LOCUS       DAOSOR010000001        39470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_1063_length_39470_cov_4.589750, whole genome shotgun sequence.
ACCESSION   DAOSOR010000001 DAOSOR010000000
VERSION     DAOSOR010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 39470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39470
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1063_length_39470_cov_4.589750"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="PK710_00005"
     CDS             <1..1272
                     /locus_tag="PK710_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HOT08112.1"
                     /translation="LAAVVLPGAKGFVDVPGKGKLKVEEVLQSGGAQPCAEYSGAMQI
                     PLPLGTKARLELPGAAPGSGLVFQVATVNAGRKAPAGIFSSSRDWSTALYVGLSLAVH
                     IGLLSAMAFFMPPLGLTDEGAMSKDQLYLIQQYLNASAEREMEQKETDQVAEDNPDNK
                     EGGTGTRAKGEEGSMGDPNSRNTNRRYGVAGPKDNPDPHIARSAALREAAEFGMIGLL
                     NTGAGGDPNAPTAPWGRDDSLGTDPISARGNMWGDSIGDAFGAGGLGLSGIGEEGGGR
                     GEGIGLGSIGTIGHGAGTGTGQGFGSGHGRLGGSHRTRPPQVRMGATTVSGRLPPEVI
                     QRIVRQNYGRFRLCYENGLRNNPNLQGRVSVRFVIGRDGSVSNVGNAGSDLPDPAVVS
                     CVTRAYYGLSFPQPEGGIVTVVYPIMFSPGQ"
     gene            1516..2424
                     /locus_tag="PK710_00010"
     CDS             1516..2424
                     /locus_tag="PK710_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT08113.1"
                     /translation="MKLSTLCVMMFFGFVVALSGCGREKIEAVNLANEGTLLKKQGAY
                     DAAIDKYEAATQLDPSNHEIIYMLATTYRKKEEWEKVASTLARATQAAPQYANYHYER
                     GYALVQVASKSKQRSAWEEAKEPLVKCIEADTRFAHCYFELALVELFLDNEQGALENY
                     TKAIQHAPNEGSFYAPLADLYIRLDYFDQAKGVLEQGVKFAADESKAKFNMYVLLASV
                     AQFQNDMPSMVKALETANELGGKDNPEILFNLGSTYAVLNPPKKSQAIQMLKKFQAQA
                     CKGGSAQKYREQCEQAIALLVKLEGP"
     gene            2461..13899
                     /locus_tag="PK710_00015"
     CDS             2461..13899
                     /locus_tag="PK710_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT08114.1"
                     /translation="MANRRNYLPEPTEGYMDLQQRVKQLEVVHDWQGVLEELEKAIAT
                     ESQPSNKAHLHLQIGRLLDGKFLQSVKALKHFQDAFKLQPALVEALADARRIYWRLGK
                     LNMVQKLLDLELKVESEPARVAELLLEMGDVLSDEGDYERAMSTYARALGASKGNNAE
                     ASACLEDVQIDTGGWQDRVAFLLRTAHEAAEPSEKARLFLRAGRIVKRFAPDEMEGML
                     AQAFRADPTNIQVLTLLEALLVEADRTDEIAALQRSVLDATEGADRRAELAFLFGLRW
                     ATRHQNLDLGAKLAQEALSYDPDNEAAFLFLREVYGTRDGAWEQVVELATKAFEQARQ
                     PGSRTFLLSQLGLINWQKLGNMLEAQRAFEQLASFADGHPSLVAFEAQIGQSLKPVPV
                     SAPAEISPVPPLVSADAEADVQVEVGESVEQPSDILAAQAEAEAEVTVEVAEQEEVQD
                     EPLDSAQEAEAEEQSAPLSAGDAEKIAELRQAAVTQEGSKRFNELVRTLMALADLVPN
                     VEEKIDLYQRAADLFVTKFSNQAEAIKAYEKVIEYDPGNAQAVQYLLQMYEKRRDWES
                     LIRLRLTEANGLPMDRRVAELIDIADVATKRLRKPDMCIDLWNTVLEVEPDNRVALDA
                     LAPLLENARRFEELVVVLEKQVESTYDEKAKIQLLTKLGTIYGDRLQNEEGAVEAWRL
                     LLVLQPNDRRAQEQLRRKYITLRRWDDLESFYSDSGKWDEFIRVLESEEGKETDSAGK
                     ISLLMKVAQLWNDKKDKPDRAARALEKVLALDENHLAAAEALVPIYQSADNAKGLARV
                     LEVKLSHEEDDFTKLELLREVAQLYEGKVRDPERAFERYLSAFEISPGDETCRDDVER
                     LAKAIGKWDSVIASYRRAIEVAQGDGEQELAISLHLKLGRVLVDEVNNVDDALTEFRS
                     VYEADSENPEAIAALESLYRQTSRYDELLGIYEKKRELTADFDERRQVLYSIAGLYET
                     EMGQPERAIETYVAVLEEDPTDMRALRALDGLYRGQGDFASYADVLQRLIELDVTEEE
                     LIDLKFRLGSTLEAHLSDPMGALNNYREILFVDQHHDGARGALEAMLSNEQLAAEAAQ
                     ILDPIYEVREDWEKLLGVLEVLASTQDDVATRVDILRRISRTASGQLGDLARAFSAQA
                     RALKEDPSNTEARDELEALAQESEQWTKLEEIISEIATSLTDVELARSYWLRLARIEE
                     RLDKVDAAAAGYGHVLSLDPADQEALEALEALYGRTERWEDLIGVYRKRIELMEDIAD
                     REKMYAQMAQVYETRLGRPSDAIAAYQEVLALDETSQVALEALDALYSRQEMWFELAD
                     NLESQLRLAVTDEDELALMLRLGALRESKMGQIEQAVDIYHQVLTRDMGNESALQALE
                     RLGSNADFELMIAEILEPLYRQTGDYAKLIGVHEVQVRRTDDPVRRVELLHQIASLYE
                     DAAGDLSAAFDTLARALAEDPAHEATQEALDRLARATSRFSDLAHVFERLAQAQQDGF
                     LASQLFSMAAKVYEQDLGDVDNAVKHYRKVLSIDPTSLSAAEALEGLFRGVERYADLS
                     AVLQTKAEIVDDPMEKKTALFQAAAIEEEMLNRPEEAIKVYLLALEADPDDVQAVDAL
                     IGQYLGLKRWEDLLGAYTKKVDLVFDPEEKKLIYYQMGAVYEREIGDVSRAIDTYQRV
                     LELDPDDLQALGRLDVLYQASGEWAELLTVLQHESELTMDAAEAISYQYRIAELYEKH
                     LQDVPRAIELYRDILMQQSDHDPTLQALENLKQGDGEPLAAASVLEPFYDGAGQWAKL
                     ISVLEVQVRFQDDAYLQVELLHRIAALYEDNLQDHPSAFDTYARALSLDNTNHDTLGA
                     LERLAALTDRWGQAAALYDRELDRLEDNPDWFVDLGLRTAAIYETQLEDLTNAVVRYR
                     KVLGVAPENEQALSSLDRLFELTEQWGDLAQVLVREAEVTSDPDRVLDYRYRLGQVYQ
                     LRLNDIDQAVASYRDVIVSMPEHEAALEALETLFANGVKQAEIAEVLEPLYTDNAQWE
                     KLAGVHEAQLAHLTDSDERLAMYYRIAEDYEHNLLDPVRAMEAYIRAIREFPLDERIG
                     EEIERLAADTDGGWDRLGNAYADVLDIQQDPVVRKAIGKRHARVFEEELQQIDNAIET
                     YRYVLGVDPTDSDALVNLDRIFEAMEQWAELAQVLEQRLRPSDEPVDWVELHGRLGAV
                     YEEKLQRYDDAIRVYRRIFDELDPSNEEAIAALERLYEYKQAWTDLNVVYERQLDNAM
                     GDMEEADIRAKMARLASERLGDVEASIATWKRVLDLRGEDVEALGALANLYESRGQWA
                     ELCDVLERQHDIADTDEERVRIRLRRAYVFLVRLNRDEEAIDDYQRVLEIDYANVDAL
                     RAIANIWRTRQEAYELVQALHNLVDRASDYLEAPELKSIFRELGITYGTVLEQPYDAA
                     EAWTRLLEVDPGDFEALDALEAIYRQDERYTDVIDVKMRRAEALPEPAEQIRELLEVT
                     RIWEEQLGDRDSGTLAFERILGIDPTHDDAFLALEQLHTAAERWEPLIELYLSRLETR
                     QEASERTELLRKIAGVFEEHVDDKNQAFEALLQAFEDDYGDDETSRYLERMAQATNRW
                     SELIQTANTWLQAEQEKQDQGKVIQLCLRLAKWYGEDLGHAQYAQPYFQKVMQLDPTN
                     VAVLRQMGNLYRKNQQWQELGRTLTEALKHATSEKDRKEIQTELGELLEKQLGETEKG
                     MVFYKNALEVDPHHLPALEALERIYSERDQTRDLVEILAAKVVAKQDEYEEVASLQVR
                     MGGLYETALSDLDKATMAYEAALDAEPSNLLAMRGLERIHAAHGNWPEMIKVLEQQLD
                     VVPTERERIEVLMKVAAIFEEQFLKADEAASRYEQVLDVDPNQEPALVGLERCYAKLR
                     QWLDLVNTYERHISVTLDRGLKIELYGYVAQVFSDEVQDVDRAIDAYQSIVELDDTNI
                     GALEALAKLYERKESAADAIDYMTRVAELTADGAQRVEMYYRIGRALDTQLGDRMAAR
                     ERYEMALDLDPAHLLTLAALKEIAIDAAEYDQAARYLDQEQMHTQSSRARSKLLVELG
                     NLREQQLEEHDLAVQAYELALQADPENEDAARPLLEEYVRVGRWAEADPLADMLLKKG
                     AGKLERYELHQLYAMQGKVAAELGNNDKALKAYKEAHHLDLTDQETIRGLAEVSFKMG
                     DWAAALSNYQKVLTSLEESDREDRAYVYYKLGCIKREQGQLKQAVNNYEKALAIDDAH
                     RMTLEAMVDIYAGLSDWKQVCAYKRQILDNIYEAEERFAMLNEVGDIWAEKENNLPKA
                     IETLEEALELKPDNHVLLHKLLGLYQKTRNYDRMIDTIQTISDLDSNPERKARYLYTM
                     AQLHRDQNDLGRAVELFNEALDLNPDFLEAFERINKILTSQKDWKQLERSFRKMLHRI
                     AGKGKVDLEYNLWHNLGIVYRDRLNEVKEAIEAFRMASRLKPEDITERQILAELYETA
                     NQLDLAVEEQQEILRRDPTQKAPYRALYQLYYQKQAFDEAWCMCSALSFLHLADEDQQ
                     KFYEDYRPKGIPPVRSRLDNESWRKYVFHRTEDTLIGSIFEMLTGAALMAKMQQLKAA
                     NQLPVLDKRYKQEAHTTTVTFAKTFFHVAKVLGITAPELYVHTNIQGGLTAAPIMPFA
                     SVAGQSVLSGFTPHELSFIVAKHLSNYRGEHYIKNIFATQSELTQLFFAGLRMVLPDA
                     PVPPEMAQNVDVTAKTLAAMMQPQHREGLRHVVRTFMETKGAINLKRWLQATELTACR
                     AGLVVCGDLEIARKILSIEPQVPGDLTPEDKITEMIVFSVSNEYFAVRKALGLTIG"
     gene            complement(13984..14613)
                     /locus_tag="PK710_00020"
     CDS             complement(13984..14613)
                     /locus_tag="PK710_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08115.1"
                     /translation="MARAEQEQHEDPVVLRPAPDSLTNHIAISPKIAFIVPTGSAEKR
                     FPQRAYVQAGPSYGLDLAYGLSRYVALQARFEYGMLSANDQCPNGGTCEATTLAFGAG
                     VDYHLVHGAAFDPWLRAGMGYRWTRFDLRWEGINRDRAYAGLDWLHLAIGGDWYPHPM
                     IGLGPFFALDIGSYQARPDSPPPGTSGPAQATVHTFVSLGLRAVFDPMR"
     gene            complement(14749..15435)
                     /locus_tag="PK710_00025"
     CDS             complement(14749..15435)
                     /locus_tag="PK710_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08116.1"
                     /translation="MPRFAPIRVPCAAISVVFATSSLLAAAPSQNNTASQPSSHEQDQ
                     TPFPSPAHPFRLEATAGAGFGSTTLSSGETINAYGPGLGLHAGYTFEIGVAIALQYQH
                     FFGTTSSYPIPLVALIEHRTGASFASVDLGFEFTPSHAIFRPHVGLGIAGLRKSIECS
                     PVDGSFSALARDVCHDYHASHGSWSKAIAPGLLMGLGFDRYYGFVDLQYLIPDDAEAF
                     IVTGGFGLTL"
     gene            15564..16748
                     /locus_tag="PK710_00030"
     CDS             15564..16748
                     /locus_tag="PK710_00030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT08117.1"
                     /translation="MQLALVTSQFPSLTGSGIPGRAAQFALALSREGAVHLFSALSEQ
                     DLESEAKRGGATIEPYARAITSVMESRRQPERRGPAAVQRFPERLSRALARADDAKPF
                     DVVVVIHSLSGCVLGPLTKAVVVVDEPELRSSRENRALVDLGTRTLSRWLGARQGEGH
                     ERRVWRRADAISVANDRDLLQVQQVRSDLGVVVPNGIDTHTGCYTPPSLRSGNRILFV
                     GDLCASPIVRGVKELVGEVLPRVRVQVPDASLTLAGQKVCREVRALESAHVRVRGMDE
                     DFSRLMREHATYAIPMTSGPDGWKGVLEPMACGLPIVAPPTLLREYGMQQGVGYAVCS
                     SIAQMADSLIAVLKGRSGFDPMALSARRTAERYDWQIVGDQFVQLVMAAISRKKRLGR
                     QK"
     gene            complement(16739..18526)
                     /locus_tag="PK710_00035"
     CDS             complement(16739..18526)
                     /locus_tag="PK710_00035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOT08118.1"
                     /translation="MSTDWNAEFNITTFREEATPLLTVIRHANHVETARTRLFDRVTR
                     LQYDTFSHSEYKHLETVYVRDCVRTFRSILEARSDSLSGFSVTQALFDIARGSDRPDL
                     SPAFFAELTHLFRGVQRKLPARLANDLKMNHAIEGREAAIARSDELDRLFQRASWLMD
                     RYTSGLDPQSRQRRVERRDRVLDALGATLDDWNDYRWQISHVLRDPDDIGRCVTLTDE
                     EKLSISRLRRQRLPFGITPFYLSLMDDEPFVRDRAIRAQVLPPLSYVDSMRKDHRASC
                     GLDFMLERDTSPIDLVTRRYPGIVILKPFNTCPQICVYCQRNWEIDDAMMDNALAPSH
                     KIDKAIAWIADHPAIHEVLVTGGDPLALDDDNLLNILERVAAIPTIERIRIGTRTPVT
                     VPMRLTDRVLDRIAAFQEPGRRELCFVTHVEHPYELNADLIAAIERIRRRGMNVYNQN
                     VFTFFVSRRFEAALLRRQLRLVGIDPYYTFNTKGKEETEAYRVPVARLLQEQHEEARL
                     MPGLSRTDEAVFNVPRLGKNHLRARQHRDLISILPDGARVYEWHPWEKNIANQTTYVG
                     RDVPVLEYLDRLANIGEDISLYSTIWYYF"
     gene            complement(18700..19887)
                     /locus_tag="PK710_00040"
     CDS             complement(18700..19887)
                     /locus_tag="PK710_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOT08119.1"
                     /translation="MSTSVVIDFSPLDTPTQRRGIGRYLRSLALGLSRLDPNASKDIR
                     PIGLLGLSGIGVPIISEDFHCIATHAWKRAPTQFDRYRWAYTRRLALWKAMQKLNARL
                     VHLGDPNATPLAMSFAQCKRVATCHDLIPLQFPKQYLGVRDGFGVIGKRLIKRRFHSA
                     DHVVAISNATRQQLIKLLDIPHDRISLVYNGVDLSTFSPHDPGDDQRRLARFALTPRK
                     YLLYAGDLDWRKNTEGMIKSLAFAHRLGSDLILAFVGDLDASKIEELKRLASYHGVDN
                     RIRRLGFVSDTDLRALYRQALAHLLLSRFEGFGYTVIEAMASGCPVITTRGGSLEEIA
                     GNAAIVQSPDDTEAIGQAIHSLHVDPARRESLISAGLDWAPRFSCEIHASHMLDVFRS
                     VLA"
     gene            complement(19904..21091)
                     /locus_tag="PK710_00045"
     CDS             complement(19904..21091)
                     /locus_tag="PK710_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOT08120.1"
                     /translation="MKTVLVDLSALDTSLRLRGIGRHVQLLASGLSRIVQSDDCGLRI
                     IGLVHLSPFANDNALHDLQHIEFDPMPPRASLLDDQKTQWFRRLILQRAAKNARADLI
                     HLPDPNAPPLRFTRSPLQLVTCHDLATVRSNRPWLRFTTSVHKRLMAHRYLSADHIIS
                     ISSYTAEDLRTLFGLPSSRISTVYNGIDSNRWSSEKNEEDARICQKYGISNTPFALYV
                     GHASWRKNQSGMLRALRYARELKPDLGLQLVWAGRLSVDEFSAIDREARNLGIRHALR
                     LLNFVSDEELACLYRCAAAHLLVSRHEGFGLTVVEAMACGCPVITTRRTALAEVAGDA
                     ALHVEPDDHEHIGKALLRLIEDHDLRRDIVARGIERAIQFSVERQARETLAVYQRVLG
                     IRP"
     gene            21255..22496
                     /locus_tag="PK710_00050"
     CDS             21255..22496
                     /locus_tag="PK710_00050"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT08121.1"
                     /translation="MYWIDVGDGNVHRSYAVGEGVRLVVGSAKASDIVLMDRMVSARH
                     CELRLEDGVLWVRDLGSKNGIYAGGARVDCARLGEGSCFSVGRVACCVRSESESMESC
                     LPLPGVVGTSAAMMRVAAQVRRVAKLSIPVLIRGETGTGKEVIAQTIHALSTRKEGIF
                     FGVNVGALPPSLAAAELFGHDRGAFTGAGQVRKGAFQAAEGGTLFLDEIAEAPLDVQV
                     LLLRALERKEVLPLGSDRHVAVDVRIVAATLAGLEEAVAKRRFREDLLHRLAVGTVQL
                     PSLRERKSDIGILAEHFLWQRRDEVGVKRLTPGAMGRLMVHDWPGNVRELSNIVFRAA
                     AQTDGEWVRARDVDVCLAARRTTVGPLSPTAAEALVIACRGNISIAARKCNVARSTFR
                     GWLNAGKSDRKKGKWPSERQE"
     gene            complement(22561..23679)
                     /locus_tag="PK710_00055"
     CDS             complement(22561..23679)
                     /locus_tag="PK710_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08122.1"
                     /translation="MRIRASAFLLVALSMPVHGSIPNAMAQTADTAFTDSARQRFQEG
                     VRLFDQGKFEEARAAFLQAYALKQHPAVLLNLAQSELHSDHPVEAARHFSEFLRDNPN
                     VEAEERAAAEQGLAAARTKTARLDIKANLDGVDVFVDGTLIGRTPLFDDVDVAPGSRT
                     VELRAGGKPPQSKTVDAPIGQVRTVMFSFDLSATPPVGAPETESSEPPESTKQEGRMP
                     FFDWLISDQVAWASGGATVLGLGTGILFAIFSHSASNDADSIASQIKLVASRDDELGD
                     RQKNPCADPVPITSRTNYGPACRQLKDNLDIRDRDKTVATIGFVVAGVGAISTGVLYY
                     LRTEPEDSAASTSSAVRSTTILPLISSDYQGLSIGGTF"
     gene            complement(23713..24633)
                     /locus_tag="PK710_00060"
     CDS             complement(23713..24633)
                     /locus_tag="PK710_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08123.1"
                     /translation="MSEATSGSIPKSIPDEQDVPAPRDSVPPLSAEEAERAASQFKPA
                     WETSIDDASPPADITHEPIDEQVVSADEDKTQPPESAVTSPHPEPPADERIDDDQGPV
                     DIIAAIPKRTEIVIKDEPSIVVPDDDPFDADASPKTAESEAQPDLQPSQRRTPVAVIA
                     VVAVAAVAIVVGGIKLWSSDPVEPTQSTTSSRPAESATSVDTLPIAQEPVGSTSIQEP
                     TPTVEPPIAPPPEPVVSTDTRQPPTKQPSAPTTVDAPPKPKDKDPDREPKPVTTATVA
                     PEPKPPKPPKTSAPSPGTKAPPVIVRETPF"
     gene            24757..26520
                     /locus_tag="PK710_00065"
     CDS             24757..26520
                     /locus_tag="PK710_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOT08124.1"
                     /translation="MNSKNVAQDRRRWLGSHDGMRSVAQARGWWAAALMMVLNGLGVG
                     CSSEKTEEPSALSIVDASRMEADIQGFPEYEHANNIMASDHPFTSRAQAVTIADQLSL
                     ALEGKKPPQTLLGAELVGDLRRRAYRTFHQPADAREAILQYDMVTRGQDAQRGCISRL
                     ASLALRGEIEDNLDSIYLSIYEETKKQLPVPCEGLARRALEDLRAYRPDASRLAEIDR
                     RFHQAQVDGGAGKEAVVKPGPETRAGPSKIESIEVFSSRDAARVVIQLTAPALYEVVS
                     LGAEGAQGPRVYVDLQKTKRGQAPRERRAEGLVTAVRLGTHQDKTRVVLDLSQQAFKR
                     VFYMPEPFRIVLDISTTAPISERAKGETGPRQVRRVVLDPGHGGSDPGAIGPGGLKEK
                     DVTLDIAHRVAPILSREMGIVTMLTRDDDRYVALEERAARANAFHADLFVSIHCNAAE
                     DPSHRGVQTFVLAGAKDEASRRLAARENAASMAAGQQMGAVLDELHLESVSHQSWQFA
                     TLLQRATLSSLSESYGAVPDGGVRSAAFFVLVGAQMPAALFEVSFVSNPQEETRLATV
                     DYRQKLADGVVNAIRAYRDGR"
     gene            complement(26532..27110)
                     /locus_tag="PK710_00070"
     CDS             complement(26532..27110)
                     /locus_tag="PK710_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT08125.1"
                     /translation="MDAKDHNNPELFTASDVARFCQVDLKTIHNWADKGEIRHFRTPG
                     RHLRFRRLDVLDFLRKYGYPIPEVLRLGKPKVVAVDEDANVLAALRRVLTRRFELTTF
                     QDSFDALVAVGNLQPDAIILDVKTSGLDGLRLLERLKSIEATAHIRSIVYSGHEDMRK
                     PALEAGAYDFILKGEVSELRDSLERLMGLDRT"
     gene            complement(27252..28118)
                     /locus_tag="PK710_00075"
     CDS             complement(27252..28118)
                     /locus_tag="PK710_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOT08126.1"
                     /translation="MIKKTKQSGDITPMGEPKPPLAATVLVVRERKGSLQVLCVRRHP
                     NQRVWGGVVAFPGGKVDPEDATAPADAVHPRIFEFSDDPTTERACAVAAARELFEETA
                     LLPTDEVEMPSAPGSRSWNQKLRLRYEPGRLWTLLASSAIELRLSQLVPVSRWVTPPM
                     LPVRFDTRFFAMRMPLGQEILRVGTENMDCFWAFPDEILDQFERHELEILPPTQWMLF
                     YISKFNSLDKFFEAISKLPLHIVIPEIFQNNDRTLLVLPGHPGHSQRPRVVVEPTYFE
                     YCLGQFIINRKN"
     gene            28247..28609
                     /locus_tag="PK710_00080"
     CDS             28247..28609
                     /locus_tag="PK710_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOT08127.1"
                     /translation="MNKAQLKKFRALLEQKREELVRKAKQTLDEDMTLDVSDLPDEMD
                     LASSEYLQSFTFRLRGREKSFLDKINKALAKIDDGSFGVCEECGDEISVKRLEARPET
                     TLCIRCKEDQERLEKDFG"
     gene            complement(28688..31588)
                     /gene="uvrA"
                     /locus_tag="PK710_00085"
     CDS             complement(28688..31588)
                     /gene="uvrA"
                     /locus_tag="PK710_00085"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOT08128.1"
                     /translation="MHPLVVRGARQHNLKDVSCDIPRNQLVVITGPSGSGKSSLAFDT
                     IYAEGQRRYVESLSAYARQFLEQLAKPAVDAIEGLSPAIAIEQRALGKNPRSTVGTVT
                     EIADYLRLLFARAGTPHCPTCGKRIEAHTVQQIVDGLLALSEGSRIILLAPLCRGLRS
                     DLHEEINRLRREGFVRARIDGTIIDIGEELRIDPAQPHDLDVVVDRIAVRDGIKGRVT
                     DSVELALELGEGRLLIDDMSGADPIWKSERFACIDCDVSFPAIEPRMFSFNGPHGACP
                     VCGGLGSRTRIDPDRIVPDDSLSLREGAVVAWGPRGSLALATEVAKAVRALKVNPNLA
                     FRELEESTKHAILFGIPKTTRRKSDYEGIVPRLERRLAGVDEEPSEDDSDLDDDTAEV
                     DPSHFAATSTCDACHGRRLRREALAVRVGNRNIAEYGELSLGNLRTVLQELLAKDSGL
                     SHRERAIAEPLLRAVIARLGFLIDVGLDYLCLDRATQSLSGGEGQRIRLATQIGAALV
                     GVLYVLDEPSIGLHARDNAKLLDALRYLVRLGNSVIVVEHDRDTIAAADHVIDMGPAA
                     GVHGGKIVAEGSPSQIQQCPDSVTGPYLSGQKRIPLPAKRRKPSQSVLRIVNAHAHNL
                     KHITVEFPIGLMTAVTGVSGSGKSTLVIDTLLQAVRSNLYRACSTVGSCDRIEGLSHI
                     DKVIAIDQAPIGRSPRSNPATYTGVFTLLRELYAALPEARARGYKAGRFSFNVKGGRC
                     EACQGDGVLRVEMHFLPDIFVRCETCGGRRYNRETLEVQYRGLSIADALDLTVERAVE
                     VFEAVPRIRERLLALSRVGLGYLQLGQSSTTLSGGEAQRVKLARELARRATGRTLYVL
                     DEPTTGLHFSDITVLVEALLALRDAGNTVVIIEHNLDIVACSDWVIDLGPEGGDRGGH
                     LVATGTPHRIAQCETSQTAPYLARVLEEQAAPSRKHKTEAVASKGRQHRPGT"
     gene            31847..31920
                     /locus_tag="PK710_00090"
     tRNA            31847..31920
                     /locus_tag="PK710_00090"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31881..31883,aa:Ile,seq:cat)
     gene            31965..32702
                     /locus_tag="PK710_00095"
     CDS             31965..32702
                     /locus_tag="PK710_00095"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOT08129.1"
                     /translation="MSIQEHIVYLEELSALDEEIRAVDDTLSTERSALAGKKEELARL
                     EEKIASDEKSIAEMAKTRTDLVLEVRQVTALVEKSREKLSRARNERETNAATRELEEL
                     RNIQKGHEEQIGKLQLLETEASKSKEEAEVARRALEDQLGTTEGVVSKRLAEAEQQKA
                     QMLERRQGVVTKLPRPILSRYEMIRKRRGIAIAATHNGICEACHLAAPPQLFQKILRQ
                     ETFEVCPHCQRILYFKPSDAESNKEER"
     gene            32711..34015
                     /locus_tag="PK710_00100"
     CDS             32711..34015
                     /locus_tag="PK710_00100"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT08130.1"
                     /translation="MKSCPVCRRLFPDDAGFCPVDGEALLSAAEVQPAVDPDDARVGQ
                     LLANRYQVRRIVADGGMGRVYEALDLQAERRVALKILHPAVVRDRVAVERFKREYEIS
                     KLLPHEHIVEVFDYFALDDGTYALAMDFLEGEELRSLLKREKTVRPARLIRMLSQIAI
                     GLDEAHQRQIIHRDIKPDNVFVCGTREGDIIKLLDFGSVKDKSEGAKKLTVMGTTIGS
                     PYYMSPEQAQGLDTLDARADVWALAAISYEVLTQQVPFPGKNGTAILLAILTQEPIPA
                     SRAARDFKEAYPIPPTVDAVLKEAFAKDPIMRVASVGLLADKLGHAFGLEGMHRDWAY
                     ADELDLHARIESVLPELMRAAPEPEPLPTTPVATVEADPFALTTSDAKPSPAGEKAGS
                     STEELRAVVVPKRLPIALIVGLFVGGLMVLLVGAVLVILWLR"
     gene            34044..34499
                     /locus_tag="PK710_00105"
     CDS             34044..34499
                     /locus_tag="PK710_00105"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOT08131.1"
                     /translation="MKAKSFSLLDQWGNRRTLEQFEGTYVVVYFYPKDFTSGCTKEAE
                     AFRDAAERFEALGAKIVGISKDSVESHAKFANKLGLSFPLLSDPDLGVTERYGAIGPK
                     NMYGKIVQGVLRSTFLIGPDGTLLRSWNRVRVPGHIEAVLEALARYKTS"
     gene            complement(34510..35682)
                     /locus_tag="PK710_00110"
     CDS             complement(34510..35682)
                     /locus_tag="PK710_00110"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT08132.1"
                     /translation="MSQPFDIPIGSTLIVCLALGCSPRSCSNSSSTDHPDALVVALPE
                     PERPPVPRKRMVWIPPGAFVAGTPTDVLPRIADEEMPGVQVVLHGFYIDQYAFPNEPG
                     AIQTTNITRDEAKARCEAIDKRLCTELEWEAACKGPNSTTYEYGSAYNPSICAMSKTG
                     TMAPTGSSAGCRSGFDVADLHGGAFEWTSSPWNRGESSTLAVVRGGSGEPAEVVGRCA
                     NARARRPDKSYADVGFRCCAGEPNDAEVSLEVSRPSDPLKAVARTPELVSLLEQGLPE
                     KITQQLSETKPFRIDRIWKWYPIGNEEIVLGSGCAHPTAHALCGVVLMRIQNHQPQTL
                     AFAPSGWWLPNVQLDDDRRVLWVYGGDGLGKYRRRVAFLWGRIGVGEPELGGVKVR"
     gene            complement(35679..37112)
                     /locus_tag="PK710_00115"
     CDS             complement(35679..37112)
                     /locus_tag="PK710_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOT08133.1"
                     /translation="MMLPSKDQVLVVDDEANIRRVLSAQLAREGYEVHTAEDGERALA
                     ILREHHIDLVITDLRMPKMDGMALLRHAIEIDPDLPVVMLTAHGTVDNAVEALKTGAF
                     DYITKPFDQTELRAIVAKGLRTRNLANAELGLDARAHVEGAKFGIIGDSKPLQEMYSV
                     IERVADTPTTVLITGESGTGKELVARALHENSSRRDKPFIKVNCAAIPKDLMESELFG
                     YERGAFTGAVNAKPGRFELASTGTLFLDEIGEIPIEMQVKLLRVLQESEFERVGGIKT
                     IQVDVRLVASTNRDLKKEIAAGAFREDLFYRLNVVPIELPSLRHRREDIPLLTQHFIQ
                     KFNERLNKSVAGVEKESLELLRQYSWPGNIRELENVIERAVLFCDGTNITPADLSPEV
                     RETQTPSCPPVVSVSIEDAVLSSDGLKEQVRAARTRVERELIVRALEQTAYNVTHAAR
                     ILKISRKGLQLKMKELGLRDREEKPDA"
     gene            complement(37127..38578)
                     /locus_tag="PK710_00120"
     CDS             complement(37127..38578)
                     /locus_tag="PK710_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HOT08134.1"
                     /translation="MFRQALPLLLITTCTSCSWWSRFDDLKDHPPVVEMENPGGIVGF
                     GTTMATVTTEVGTHLLVLGEPNVSPKGATYRITNDTPATTPNASGFCPTGRCLFGSTP
                     AALQTMLIDGQPHEACFLLGVGDRDDTLGLLGHCSDGLDFVLPVPGRDEAHPTVKSPR
                     DVIAEDLALQSLDKLLIGDFKIAASKDDVPLIAAATRHLGAAWFYAPATTVPILLSPV
                     AAPGLPLEDPFLTFGDTFGSEVAVVKLSEGGYLLAIASPHGIGNDTQGRVWLYRIVEG
                     KVVHDGPHPDVPAPMSCLPGFRHNFGIHMRSGKLFEGSSEQLFISDGVTISIYDADDL
                     AKYPPSVDCSGAPLGSTGFITGVNCVETASVSGCSRITNEFGRALAIADVNGDGSKEL
                     VIGVPTLDVDGTNTAGAALVYHVDPEGKQTRLLETRIISSRDSGDGLGSSVAAMRIGS
                     RDVVVAGAPGLGSFFIFFCSTTGGAGHDSSRCE"
     gene            complement(38578..>39470)
                     /locus_tag="PK710_00125"
     CDS             complement(38578..>39470)
                     /locus_tag="PK710_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08135.1"
                     /translation="ECSQSYLAEYSGTARGDAVMDRPARGLLGCAYDLEHGEAVFAAE
                     VISVASASRAFSVKHGEEREELVELIRGSKGTLHISGNASPIHVSVDGIPVGTFHPNS
                     AITIELPAGKHEITATADDRKDLVGQVDVPRGQVVPIDLVMVPTTPRGAAWTQAIVSA
                     VFVGGGIALKVQSDIVYDDIHTAKTNGSLTESDERYTKGKIYAYGSAACFGVGGTLAL
                     LSTYNFIKDPTPSSAIRVGVPHDYPDSDTAPPRLSAPRSPAPAGKTAPRTTSPVAPPA
                     LAIVPSYPWQGVGLMLQGRF"
BASE COUNT         8514 a         9409 c        12880 g         8667 t
ORIGIN      
        1 ttggcggcgg tcgtacttcc tggggcgaag gggttcgtgg atgtaccggg caaaggcaag
       61 ctcaaggtgg aggaggtgct tcagagtgga ggcgctcagc catgcgcgga gtattcgggg
      121 gcgatgcaga ttccgttgcc tcttggcacg aaggcgcggc tcgagcttcc tggggcggca
      181 ccgggcagtg ggttggtgtt tcaggtagcg acggtcaacg ccggtcgcaa agcaccggcc
      241 gggatattct cgtcgtcccg tgattggtcg accgccttat atgtcggatt gtcgcttgcg
      301 gttcatattg ggttgctgtc ggcgatggcg tttttcatgc cgccgttggg tcttacggat
      361 gaaggagcga tgagcaagga tcagctctac ttgatccagc aatatctcaa tgcttctgcc
      421 gagcgtgaga tggagcagaa ggagacggat caggtagcgg aggacaaccc ggacaacaag
      481 gaaggtggga cgggtacgag ggcgaagggt gaggaagggt cgatgggtga tccgaactcg
      541 cgcaacacga atcgtcgtta tggtgtggcg gggccgaagg acaacccgga tccgcatatt
      601 gcgcgttcgg cggcacttcg ggaggcggct gaattcggta tgattgggct cttgaacacg
      661 ggagcgggag gtgatccgaa tgcgcctacg gctccgtggg ggcgcgacga ctcgttgggt
      721 acggatccca tcagtgcgcg tgggaatatg tggggagact ccatcggtga tgcgtttggt
      781 gccggtggtt tggggctatc gggtattggc gaagagggtg gtggtcgtgg cgaaggaatc
      841 ggtcttggtt cgattggtac gataggtcat ggtgcgggaa ccgggacggg gcagggattt
      901 ggctctgggc acgggcgtct tggtggttcg cacaggacga ggccgcctca agttcggatg
      961 ggagcgacga cggtgagtgg gcgtttgcct ccggaggtca ttcagcgaat cgtgcgtcag
     1021 aactatgggc gtttccggtt gtgctatgaa aatggtcttc gcaacaaccc gaacttgcag
     1081 ggtcgtgtat cggtacggtt cgtgattggc cgtgacgggt cggtgtcgaa cgtgggcaac
     1141 gcggggtcgg atcttccgga tccggcagtg gtttcgtgtg tgacgcgagc ctactatggc
     1201 ttgtcgtttc cgcagccgga gggtggcatc gtgacggttg tctacccgat catgttttct
     1261 cctggtcaat gagcttggcg cggggccagg ttccttctca ctcccgatct ccctagactt
     1321 ttcatgccgc gtccgcgtag acttcccgcg ccctgtgcgt gatacggggt gcgcggttgc
     1381 gtgtggcttt cttattgctg tttcgaatag aactggttgg taggctgtag ccgcgccgcg
     1441 tactttcgct tctcgaggcg ttggttcggt ctggattgga gttgaactct gctcgttgga
     1501 aaggtggcag aacagatgaa gctcagcacg ttgtgcgtga tgatgttctt tggtttcgtt
     1561 gtggctcttt cggggtgcgg tcgcgagaag atcgaagccg tcaacctggc caacgagggc
     1621 acgctgttga aaaaacaggg cgcctatgac gctgcgatcg acaaatacga ggcggcgacg
     1681 cagttggacc cgagcaacca tgagatcatt tacatgctag cgacgacgta tcggaagaag
     1741 gaggagtggg agaaggttgc ctctacgctt gcccgagcga cgcaagctgc accgcagtat
     1801 gccaactatc actatgagcg aggttacgcg ctggtgcagg tggcttcgaa gtccaagcag
     1861 cgcagtgcgt gggaagaggc gaaagagccc ttggtcaagt gcatcgaggc ggatactcga
     1921 tttgcgcatt gttacttcga gttagcgttg gtggagctgt ttttggacaa cgagcagggg
     1981 gctttggaaa actataccaa agcgattcag catgcgccca acgagggatc gttttacgcg
     2041 ccgctagcgg acctatacat tcgtttggac tacttcgatc aagcgaaggg agttcttgag
     2101 cagggggtta agtttgcggc ggatgaaagc aaggcgaagt tcaacatgta tgtcttgctt
     2161 gcgagcgtgg cgcagtttca gaatgacatg ccaagcatgg taaaagcgtt ggaaacggcc
     2221 aatgagttgg gcggcaagga caatcctgag attctgttca accttggcag cacttatgcg
     2281 gtgctgaacc cgccgaagaa gtcgcaagcg attcagatgc tcaagaaatt ccaagcgcag
     2341 gcgtgtaaag gtggatcagc gcaaaaatac agggagcaat gcgagcaagc gatcgctttg
     2401 cttgtcaagc tcgagggacc gtaaagggag aattcgagct gaatagctcg ccccgtaggt
     2461 gtggctaacc gccgtaatta tcttcccgaa ccgaccgagg gatacatgga tttgcagcag
     2521 cgtgtgaagc agctcgaagt ggtgcatgat tggcaggggg tgctcgagga gcttgaaaaa
     2581 gcgatcgcaa cggagtctca gccatccaac aaggctcacc ttcatctgca gatcggccgg
     2641 cttcttgatg gcaagttctt gcagagtgtc aaggcactga agcatttcca agacgcgttc
     2701 aagcttcagc cagcgttggt ggaagcccta gcagatgctc gccgcatcta ctggcgtctt
     2761 ggtaagttga acatggtgca gaagctcctc gatttggagc tgaaggtaga atccgaaccg
     2821 gcacgggttg ccgaattgct tctcgaaatg ggcgacgtcc tttcggacga gggcgactat
     2881 gagagggcga tgtcgaccta cgcgcgggcg ttgggggcgt cgaagggcaa caatgcggaa
     2941 gcgagtgctt gtttggagga cgttcagatc gacacgggtg ggtggcagga tcgggtggct
     3001 ttcttgttgc gcacggcgca cgaagcggcg gagccatcgg aaaaagcgcg tctattcctt
     3061 cgcgccggtc ggatcgtgaa acgatttgct cccgatgaga tggaagggat gttggcgcag
     3121 gccttccgtg cggatcctac caatattcaa gtattgacgt tgttggaggc gttgctggtc
     3181 gaagcggatc ggacggacga gatcgcggcg ttgcagcgtt cggtattgga cgccacggag
     3241 ggagcggatc ggcgggctga gttggccttt ctgtttgggc ttcgatgggc gacgcgacat
     3301 cagaacttgg atcttggggc gaagcttgcg caggaagcgc tttcgtacga tcccgacaac
     3361 gaggcagcgt ttctattttt acgtgaggtt tatggaacgc gtgatggagc gtgggaacag
     3421 gtggtcgagc ttgcgacgaa agcgtttgag caagcccgac aaccgggaag ccgcacgttt
     3481 ctgttgtctc agcttgggct catcaactgg cagaagcttg gaaatatgtt ggaagcgcag
     3541 cgtgctttcg agcagctcgc gtcgtttgcg gatgggcatc cgagcctggt ggcgttcgag
     3601 gcgcagattg gtcagtcctt gaagccggtg ccggtatcgg cacctgcgga gatttcgccg
     3661 gtacctccgc tggtgagtgc ggacgcggag gcggacgtcc aggtggaagt aggcgagtcg
     3721 gtagagcagc catcggatat cctagcggcg caggccgagg ccgaagcgga ggtgacggtt
     3781 gaggttgctg agcaagaaga agttcaagac gagccattgg acagcgcgca agaggcggaa
     3841 gccgaggagc aatcggcgcc gctgtcggct ggggatgctg aaaaaatagc ggaactgcgt
     3901 caagcggccg tcacgcaaga ggggagcaag cgtttcaacg agttggtgcg aacgctcatg
     3961 gccttggcgg acttggttcc gaatgtcgaa gagaagattg atttgtatca acgagcggcg
     4021 gatctgttcg tcaccaagtt ttcgaatcaa gcagaggcca tcaaggcgta tgaaaaagtg
     4081 atcgagtacg atccgggcaa tgcgcaagcg gttcaatact tgcttcagat gtacgagaag
     4141 cgtcgggatt gggaaagttt gatccggctt cgtttgacgg aagcgaatgg gcttccgatg
     4201 gatcgtcgtg tagcggaact gattgacatt gcggatgtag cgacgaagcg ccttcgtaaa
     4261 ccggacatgt gcattgactt gtggaacacg gttctcgagg tagagccgga caatcgggtg
     4321 gcgttggatg cgttggcgcc attgttggag aacgcgaggc gtttcgagga attggttgtc
     4381 gttctcgaaa agcaagtgga gagcacgtac gacgagaagg ccaagattca gttgttgacc
     4441 aagttgggga cgatctacgg ggatcggcta caaaacgagg agggggcggt cgaagcgtgg
     4501 cggctgctgc tcgtgctaca gccgaatgat cgtcgagcgc aggagcagct tcgacggaag
     4561 tacattacgc ttcgtcgatg ggacgatttg gagtcgttct attcggactc tggcaagtgg
     4621 gatgaattca ttcgtgtgtt ggagtcggag gagggcaagg agacggattc tgctgggaag
     4681 atttcgttgt tgatgaaggt ggcccagctt tggaatgaca agaaggacaa gccggatcgt
     4741 gcggcacgag cgttggagaa ggtgcttgcg ctcgatgaga atcacttggc ggcggccgag
     4801 gccttggttc cgatttatca atcggcggac aacgccaagg gtcttgcgag ggtattggag
     4861 gtaaagcttt cgcatgaaga ggacgacttc acgaagctcg agcttcttcg tgaggtagct
     4921 cagctttacg aagggaaggt acgggacccg gaacgtgcct tcgagcgcta tctttcggcg
     4981 ttcgagatat cgcctgggga tgagacgtgt cgtgatgatg tagaacgttt ggcgaaggcg
     5041 ataggaaagt gggacagcgt catcgcatcg tatcgtcgtg ccattgaagt ggcgcagggc
     5101 gatggggagc aggaactcgc gatctcgctt catttgaagc ttggtcgagt tcttgtcgac
     5161 gaagtgaaca atgtcgacga cgcgttgacg gagtttcgat cggtgtacga ggcggacagc
     5221 gagaatcctg aggcgatcgc ggcgctcgag agtttgtatc gtcagacatc gaggtacgac
     5281 gagcttctgg ggatctacga gaagaagcgt gagttgacgg ccgatttcga cgagaggcgt
     5341 caagtattgt actcgatagc tgggttgtac gagacggaga tggggcaacc ggagcgtgcg
     5401 atagagacgt acgtagcggt gttggaagag gatccgacgg acatgcgggc cttgcgtgcg
     5461 ttggatgggt tgtatcgtgg gcagggggat ttcgcgagtt acgcggacgt gttgcaaagg
     5521 ctcattgaat tggacgtcac ggaagaggag ttgatcgatc tgaagttcag gcttggatcg
     5581 acgctcgagg cgcatctatc ggacccgatg ggggcgttga acaactaccg tgagattttg
     5641 tttgtcgacc aacatcacga cggtgcgcgg ggagcgttgg aggcgatgct gtcgaacgag
     5701 cagttggcag ccgaggcggc acagattctc gatccgattt atgaagtgcg tgaggattgg
     5761 gagaagctgc taggggtgtt ggaagtgctg gcgtcgacgc aggacgacgt ggcgacgaga
     5821 gttgatattt tgcgtcgtat ttcgcggacg gcttcagggc agcttgggga tttggcgcga
     5881 gcattttctg cgcaagcaag agcgttgaag gaggatccgt cgaacaccga ggcgcgtgat
     5941 gaattggagg cattggcgca ggaatcggag cagtggacga agttggagga gatcatttcg
     6001 gagatcgcga cgagtttgac ggacgtggag ttggcgcggt cgtattggct tcgtctggcg
     6061 aggatagagg agcgtctgga caaggtagac gctgcggcgg cgggttatgg gcatgtgctt
     6121 tccttggatc ctgcggatca ggaagcgttg gaggcgttgg aggcgttgta tgggcgcacg
     6181 gagcgctggg aggatttgat tggtgtgtac cgcaagcgca tcgagttgat ggaggacatt
     6241 gcggatcggg agaagatgta cgcgcagatg gcgcaggtct acgaaactcg tttgggtcgt
     6301 ccttcggacg cgattgcggc ctatcaagag gtgcttgcgc tggatgagac gagtcaagtg
     6361 gcgttggagg cattggacgc gttgtattcg cgtcaggaga tgtggtttga gttagccgac
     6421 aacctcgagt cccagttacg gcttgcggtc accgacgaag acgaattggc gttgatgctt
     6481 cgtcttggag cgttgcgcga gtcgaagatg gggcagatcg agcaggcggt ggacatttac
     6541 catcaggtat tgacccgcga catgggcaac gaatctgctc tgcaagcgct cgagcgattg
     6601 ggctccaacg cggacttcga gttgatgatt gcggagattt tggagccgct gtatcggcag
     6661 acgggcgatt atgcgaagtt aatcggggtg catgaggttc aggtgcgtcg caccgatgat
     6721 ccggttcggc gagtcgagtt attgcatcag atagcttcct tgtacgagga tgcggcgggg
     6781 gatttgtcgg ccgctttcga tacattggca cgggcattgg ccgaggatcc agcacacgaa
     6841 gcgacgcagg aggcgttgga tcgtcttgct cgggcgacct cgaggttttc ggatcttgcg
     6901 catgtgtttg agcggctagc ccaggcgcag caggatggtt ttttggcgag ccagctattt
     6961 tcgatggccg ccaaggtata tgagcaggat ttgggggatg tggataatgc ggtgaagcat
     7021 tatcggaagg tgttgagtat cgatccgacg agtctatcgg cggcggaagc gctcgaaggg
     7081 ttgttccgtg gggttgagcg atacgcggat ttgtctgctg ttcttcagac gaaggctgag
     7141 atcgtcgacg atccgatgga gaagaaaacg gcgttgttcc aggcagcggc gatcgaggag
     7201 gagatgctca atcgtccgga ggaagccatc aaggtatatt tgttggcgct ggaagcggat
     7261 ccggatgatg tgcaggccgt ggatgcattg atcggtcagt acttggggtt gaagcgttgg
     7321 gaggatcttc ttggcgcgta caccaagaag gtggatttgg tgtttgatcc cgaggaaaag
     7381 aagctgattt actatcagat gggcgctgtg tacgagcgcg agattgggga tgtatcgcga
     7441 gcgatcgaca cgtaccaacg ggtgttggag ttggatccgg acgacttaca agctcttgga
     7501 cggttggacg tattgtatca ggcgtcgggg gaatgggcgg agctattgac ggtattgcag
     7561 catgagtcgg agcttacgat ggatgctgcg gaggcgattt cgtatcagta tcgaatcgcg
     7621 gagttgtacg agaagcactt gcaggacgtg cctcgtgcga tcgagttgta ccgtgacatc
     7681 ttgatgcagc aatcggatca tgatccgacg ttacaggctt tggaaaacct caagcaaggc
     7741 gatggggagc cgttggcggc ggccagtgta ttggagccgt tttatgatgg ggcggggcag
     7801 tgggcgaagc tgattagtgt gttggaagtg caggtacgat tccaggacga tgcctacttg
     7861 caggttgagt tgttgcaccg gatagcggcg ttgtatgaag acaaccttca ggaccatccg
     7921 tcggctttcg acacgtatgc gcgagcgttg tcgttggaca atacgaatca cgacacactt
     7981 ggagcgttgg agcgtttggc ggcccttacg gatcgttggg gtcaagccgc ggcgttgtac
     8041 gatcgggagt tggatcgtct ggaagacaat ccggattggt ttgtggactt ggggttacgc
     8101 acggctgcga tctacgagac acaactcgag gatttgacga acgcggtggt tcggtaccgc
     8161 aaggtgttgg gtgtagctcc ggagaacgag caagcgctca gttcgttgga tcggttgttc
     8221 gagctgacgg agcagtgggg ggatttggcg caggtattgg ttcgggaggc ggaggttacg
     8281 tctgaccccg atcgtgttct cgattatcgg taccggttag gacaggttta tcagcttcgt
     8341 ttgaacgaca tcgatcaggc ggtggcgtcg taccgggatg tgattgtgtc gatgccggag
     8401 cacgaagccg cgctcgaggc gttggaaacg ctatttgcga atggggtcaa gcaggcggag
     8461 atcgccgagg ttctcgagcc gttgtacacg gacaacgcgc aatgggagaa gctggcgggt
     8521 gtgcatgagg cgcagctcgc acatctgacg gattcggatg agcggttggc gatgtactac
     8581 cggatagctg aggattatga gcacaacctt ttggatccgg ttcgcgcgat ggaggcgtat
     8641 attcgtgcga tccgtgagtt tcctttggat gaaagaattg gcgaagagat agagcgcttg
     8701 gcggctgata cggacggcgg gtgggatcgt cttggcaatg cgtatgcgga cgtgctggat
     8761 attcagcagg acccggtggt tcgcaaagcc attggaaagc gtcatgcgag agtgtttgaa
     8821 gaagagcttc agcagataga caacgcgatt gagacgtatc gttatgtgct gggtgtcgat
     8881 ccgacggatt ccgatgcgtt ggtgaatttg gatcggattt ttgaggccat ggagcaatgg
     8941 gcggagttgg ctcaggtatt ggagcagcgt cttcgtccct ctgatgagcc ggtggattgg
     9001 gtggagcttc acggacgctt aggtgctgtc tacgaagaga agcttcaacg atacgatgac
     9061 gccattcggg tatatcgtcg gatattcgac gaactagatc catcgaacga ggaagcgatc
     9121 gcggccctcg agcgtttgta cgagtacaag caagcgtgga cggacttgaa tgtggtgtat
     9181 gagcgtcagc tagacaatgc gatgggggac atggaggagg ctgacattcg tgcgaagatg
     9241 gcgcgactgg cgtcggagcg tcttggagac gtggaagcgt cgatagcgac atggaagcgg
     9301 gtattggatc tccgagggga ggatgtggaa gcgcttgggg cgcttgcgaa tctgtatgag
     9361 agtcgaggtc agtgggccga gttgtgtgac gttttggagc gtcaacacga cattgcggat
     9421 acggacgagg agcgtgttcg aattcgactt cgtcgtgcgt atgtattttt ggttcgtctc
     9481 aatcgcgatg aggaggcgat agacgattac cagcgggttt tggagatcga ttacgcaaac
     9541 gtcgatgcgt tgcgagcgat agcgaatatt tggcgaacgc gtcaggaagc ctatgagctt
     9601 gtgcaggcgt tgcacaactt ggtggatcgg gcgtcggatt atctggaggc accggagctg
     9661 aagtccatat tccgtgagtt gggcatcacg tacgggacgg tattggagca accgtacgat
     9721 gcggcggagg catggacgcg attgctcgag gtagatccgg gtgattttga ggcgctcgac
     9781 gctttggagg ccatctatcg gcaggacgag cggtacacgg acgtcatcga cgtgaagatg
     9841 cggcgggcgg aggcattgcc ggagcctgcg gagcagatcc gtgagctatt ggaagtcacg
     9901 cggatttggg aagagcagct tggcgatcgg gattcgggta cgcttgcgtt cgagcggatc
     9961 ttgggaatcg atccgacgca cgatgatgcg tttctggcat tggagcaact gcatacggcg
    10021 gcggagcgtt gggagccgtt gattgagctg tacttgtctc gtttggagac gcggcaagag
    10081 gcctcggagc gcacggagct tcttcggaag attgccggtg tattcgagga gcatgtcgat
    10141 gataagaacc aggctttcga agcattgttg caagcatttg aggacgatta cggggacgat
    10201 gagacgtctc ggtatctcga gcgcatggcg caggcgacga atcgctggag tgagttgatc
    10261 cagacggcga acacgtggct tcaggcggag caagagaagc aggatcaggg aaaagtgatt
    10321 cagctatgtc tccgtttggc gaagtggtac ggcgaggatt tggggcatgc gcaatatgcg
    10381 cagccttatt tccagaaggt gatgcagctt gatccgacca acgtggcggt gctgcggcag
    10441 atgggtaatt tgtaccgcaa aaatcagcaa tggcaggagt tgggtcggac gctgacggaa
    10501 gcgttgaagc atgcgacgtc ggagaaggat aggaaggaga tacagactga gcttggcgag
    10561 ttgttggaaa agcagcttgg tgagacagag aagggcatgg tgttttacaa aaacgccctt
    10621 gaagtggatc ctcatcattt gccggcgttg gaggcattgg agcggatata ctcggagcgg
    10681 gatcagactc gtgacttggt ggagatccta gcggccaagg tagtggcgaa gcaggatgag
    10741 tatgaagaag tagcgagtct acaggtccga atgggggggt tgtacgagac ggcgttgagt
    10801 gatctcgaca aggcgacgat ggcctacgag gcggcgctcg atgcggaacc ttccaatctt
    10861 cttgcgatgc gtggattgga gaggattcac gcggcgcatg gcaattggcc ggagatgatc
    10921 aaggttctcg agcagcagct cgatgtcgtt ccaacggaac gtgagcgaat cgaggtgctg
    10981 atgaaggtag cagcgatctt cgaggagcag ttcctgaagg ctgacgaggc ggcgtcgcgt
    11041 tatgagcagg ttttggatgt cgatccgaac caggagcccg cgcttgttgg tttggagcgg
    11101 tgttacgcga agttgcgtca atggctggac ttggtgaaca cctacgagcg acatatttct
    11161 gtgacgttgg atcggggttt gaagatcgag ctttacggtt acgtggctca ggtgttttcg
    11221 gacgaagtgc aggacgtgga tcgtgcgatc gatgcgtacc aaagcatcgt ggagttggac
    11281 gatacgaaca ttggagcgtt ggaagcgctt gcgaagctgt atgagagaaa ggaaagcgct
    11341 gcggatgcga ttgattacat gacacgcgtt gcggagttga cggcggatgg tgcgcagcgg
    11401 gtggagatgt actaccggat tggtcgtgcg ctggacacgc agcttggcga tcgaatggcg
    11461 gcgcgagaac gttatgagat ggcgttggat ttggatccgg cccatcttct gacgttggct
    11521 gctttgaagg agattgcgat cgacgcggcg gaatatgatc aggcggcgcg ctatctggac
    11581 caagagcaga tgcatacgca gtcatcgcgt gcgcggtcga agctgttggt ggaactgggt
    11641 aaccttcgtg agcaacagct cgaagagcat gatttagcgg tgcaggccta cgagcttgca
    11701 cttcaagcgg atccggagaa tgaagacgct gcacgtccgt tgctcgagga atacgttcgg
    11761 gtgggccgat gggctgaagc ggatccgcta gcggatatgt tgttgaagaa gggcgcggga
    11821 aaactcgagc gatatgaact gcatcaattg tatgcgatgc aaggaaaggt tgccgcggaa
    11881 ctgggcaaca atgacaaagc gctgaaagcg tacaaagagg cgcaccactt ggacctgacg
    11941 gatcaagaga cgattcgtgg gttggcggag gtgagcttca agatgggcga ttgggcggcc
    12001 gcgttgtcca actaccagaa ggtgctgacg tcgttggagg aaagcgaccg cgaagatcga
    12061 gcgtatgtgt actacaagct cggctgtatc aagcgcgagc aagggcagtt gaaacaagcg
    12121 gtcaacaact atgaaaaggc gcttgcgatc gacgatgcgc accgaatgac gctcgaggcg
    12181 atggtggaca tttatgccgg gctctcggat tggaaacagg tttgcgccta caagcgtcag
    12241 atcctcgata acatatacga ggcagaagaa cgcttcgcga tgctcaatga ggtcggtgac
    12301 atttgggcgg agaaggaaaa caaccttccg aaagccatcg agacattgga ggaagcgctc
    12361 gaactcaagc cggacaacca tgtgttgttg cacaagcttc tcgggctgta tcagaagacg
    12421 cgaaactacg accgaatgat cgatacgatt caaacgattt cggacttgga ctccaatccg
    12481 gagcgaaaag cgcgttactt gtacacaatg gcgcaactac atcgcgatca aaacgatctt
    12541 ggtcgtgcgg tggaactgtt caacgaagcg ttggacttga atccggattt cctcgaggcg
    12601 ttcgagcgca tcaacaaaat ccttacgtcg cagaaggatt ggaaacagct cgagcggtcg
    12661 ttccgcaaaa tgcttcaccg cattgcgggc aagggcaagg tagaccttga gtacaacctt
    12721 tggcacaacc tggggatcgt ttatcgggat cgtctcaacg aggtcaagga agcgatcgaa
    12781 gcgttccgca tggcttcaag gctcaagccc gaggacatca cggaaaggca gattctcgcg
    12841 gagctatacg agacagccaa tcagctagat cttgcggtcg aggagcaaca agaaatcctg
    12901 cgtcgggatc cgacgcaaaa agcgccctac cgtgcgctat accagcttta ttaccagaag
    12961 caggcgttcg acgaagcgtg gtgcatgtgc agcgcccttt cgtttttgca tctggccgat
    13021 gaggatcagc agaagtttta cgaagattat cgtcccaagg gcataccgcc ggtgcgcagt
    13081 cggttggaca acgaatcatg gcgaaaatac gtgtttcacc gcacggaaga caccttgatt
    13141 gggtccattt tcgagatgct caccggcgcg gcgctgatgg cgaagatgca acagctcaag
    13201 gccgcgaatc agctaccggt gcttgacaag cgatacaagc aggaggcgca taccacgaca
    13261 gtcacctttg cgaagacgtt tttccatgtg gcgaaggtgt taggaataac ggcgccggaa
    13321 ctgtatgttc atacgaatat tcagggagga ttgaccgcgg cgcccatcat gccgtttgcg
    13381 tcggtggcgg gtcaaagcgt gttgtctggg ttcacgccgc atgagttgtc tttcatcgtg
    13441 gcgaaacatc tgtcgaatta ccgaggcgag cattacatca agaatatttt cgccacgcag
    13501 agtgagttga cgcagctatt ctttgcggga ctgcggatgg ttcttccgga tgctccggtg
    13561 ccaccggaaa tggcgcaaaa cgtggacgtg acggccaaga cgcttgccgc gatgatgcag
    13621 ccgcagcatc gggaagggct tcgacatgtc gttcgcacct tcatggaaac gaagggagcg
    13681 atcaatctga aaaggtggct tcaggcgacc gaacttacgg cgtgtcgagc gggtctggtc
    13741 gtatgtggcg accttgaaat cgccagaaaa atcctatcca tcgagccaca ggttcctgga
    13801 gatctcacgc cggaagacaa aattaccgag atgatcgtgt tctcggtttc gaatgagtac
    13861 ttcgcggtgc gcaaagcact tggcttgacg atcggttgac gcagaaatcc gagggaatgg
    13921 accgatgaaa agcggtctgt tggcgtggga ggttgagccg acagaccgct ttctatttcg
    13981 ttttcagcgc atcggatcga aaacggcccg aagtccgagg gaaacgaagg tatggacggt
    14041 tgcctgcgct ggtcctgagg ttccaggggg gggtgagtcg ggacgcgcct gataggagcc
    14101 gatatcgagc gcgaaaaagg ggcctaaacc gatcatggga tggggatacc aatcacctcc
    14161 gatggcgagg tggagccaat caagcccagc gtaagctcga tcgcggttga ttccctccca
    14221 gcgcagatcg aatcgtgtcc atcggtaacc cattcctgcg cgcagccacg ggtcgaacgc
    14281 ggcaccatga accaggtggt aatcgacgcc agcgccgaag gcgagggtcg ttgcctcgca
    14341 ggttccgccg ttgggacatt gatcattggc cgacagcatg ccgtattcga atcgtgcctg
    14401 taacgcgaca tagcggctta gcccataagc gagatccagt ccgtacgatg gtccagcttg
    14461 gacgtaagct ctttgtggaa agcgtttttc ggcacttccc gtgggcacga taaaggcaat
    14521 tttgggtgaa atggcgatgt ggttggtgag ggaatccggg gcaggtctga gcacgacggg
    14581 atcttcgtgc tgctcttgtt cggccctagc catgcgtggt tccgtcaata ggccgacgaa
    14641 acatggcatc gacagaagga atgcaaagga tcgtgatcga aagacgcgca tgaggatatt
    14701 catgtaggcc tacttaggca gtggcagcaa tgtcgtggac gggatggttc atagggtgag
    14761 gccgaatcca cccgtgacaa tgaaggcctc ggcatcatcg ggaatcaagt attgaaggtc
    14821 gacgaagcca taatagcgat cgaagccgag gcccatgagc aggcctgggg cgatggcttt
    14881 gctccaagaa ccatgggaag catggtagtc gtggcagaca tctcttgcca acgcactgaa
    14941 ggagccgtcg acgggtgaac attcgatgga ttttctaagc ccggcgattc caagtccaac
    15001 gtgagggcga aaaatggcat ggcttggggt gaactcgaat cccagatcga cggaggcgaa
    15061 gcttgcaccg gttcgatgtt cgatgagtgc cacgagagga atcggatacg aagaggttgt
    15121 gccgaaaaaa tgctggtact gaagggcaat cgcgacaccg atttcgaagg tataaccggc
    15181 gtgcagtcca agtcctggac cgtaggcgtt gatggtttcg ccggaggata aggttgtgct
    15241 cccgaaaccg gctcctgccg tagcttcgag tcgaaaaggg tgggcggggg aaggaaaagg
    15301 cgtttggtcc tgttcgtgcg acgagggttg cgaagcggta ttgttttggg agggggctgc
    15361 cgctaggagt gacgacgtcg caaaaacaac gctaattgca gcgcagggaa ctcggatcgg
    15421 agcgaaccgc ggcattgtga gacaagagcg tagctggggt ttgtagcatc gcaaagtcag
    15481 aaagctgctc gtgtttggga tatgttggtt gagcctccaa gcctggacag gtagcattca
    15541 ttggggaggt gctcgaaaag agcatgcaat tagctctggt gacgtcgcag tttccaagtc
    15601 tgaccggttc cggaataccc ggcagggccg cccaatttgc cttggcctta tcgcgagagg
    15661 gtgcggtgca tttgttttct gctctttcgg agcaggatct cgaatcggaa gccaagcgag
    15721 ggggagcgac gatcgaacct tatgcaagag ccattaccag tgtgatggaa tcgagaaggc
    15781 agccggaacg tcgtgggccc gcggccgtac agaggtttcc ggagcgtctt tcgagggctt
    15841 tggcccgtgc ggacgatgcg aagccgtttg atgtggtcgt tgtcatccac agtctatctg
    15901 gctgtgttct tggtcctttg accaaggcgg tggtggtggt ggatgagccg gaactacggt
    15961 catctcggga aaatcgcgcc ctggttgacc ttggaacacg cactttgtcg cgttggctcg
    16021 gcgcgcggca aggggagggg catgagcgtc gggtatggcg tagggcggac gcgatatcgg
    16081 tggcgaacga ccgggactta ttgcaagttc agcaggtacg ctccgacctt ggcgttgttg
    16141 tgccgaacgg gatcgacacc catacaggtt gttacacccc tccttctctt cggtcgggca
    16201 atcgaatcct gtttgtgggg gatctgtgtg cttcccccat cgttcgtggg gtcaaggaat
    16261 tggttggtga ggtattgcca cgggtgcgtg tccaggttcc ggatgcgagc ctgacgttgg
    16321 caggtcagaa ggtgtgccgg gaggttcgtg cgttggaaag tgcgcatgtg cgagtgcggg
    16381 gcatggatga ggatttttcg aggctgatgc gcgaacatgc cacgtatgcg attccgatga
    16441 cttctggccc ggatggttgg aaaggcgttc tggagccgat ggcgtgtggc ctgcccatcg
    16501 tggctccgcc gacgttgctt cgtgagtatg gcatgcaaca gggcgtggga tatgcggtat
    16561 gcagttcgat tgcgcagatg gcagacagtc tgatcgcggt gttgaaaggg cgctcaggat
    16621 ttgatccgat ggcattgtcc gcgagaagaa ccgcggagcg atacgattgg cagatcgtgg
    16681 gcgatcaatt cgttcaactg gtcatggcag cgatatcgcg aaaaaagcgg ctagggcgtc
    16741 agaagtagta ccaaatggtc gagtacaagg agatgtcctc gccgatattc gcaagtcggt
    16801 cgaggtattc gagtacaggt acatctcgac ccacgtaggt cgtttggttg gcgatatttt
    16861 tctcccaggg gtgccattca taaacccgag cgccatcagg aaggatcgaa atgaggtctc
    16921 tgtgctgacg agcccggaga tggtttttcc caaggcgagg aacattgaac acggcttcat
    16981 ccgttcgaga caagcccggc atcaaacggg cttcttcgtg ctgttcttgg agcaatctag
    17041 cgacgggcac acgatacgct tcggtctctt ctttcccctt ggtattgaat gtgtagtacg
    17101 ggtcgatgcc gaccagacga agctgtcgcc gaagcagtgc ggcttcgaat cgacgggaca
    17161 caaaaaacgt gaagacgttt tggttataca cattcattcc gcgtcgtcga atccgctcga
    17221 tggccgcgat gagatccgca ttgagttcgt atgggtgttc gacatgcgtg acgaaacaca
    17281 attctcgtcg accaggctcc tgaaaggcag cgattcggtc gagaacacgg tccgtcaatc
    17341 gcatgggaac ggtgacgggt gttcgagtcc cgattcgaat acgctcgatg gtggggattg
    17401 ccgcaacgcg ttcgaggata ttcagtaagt tgtcatcgtc caaagccaaa ggatcgcctc
    17461 ccgtcacgag aacttcatga atggcagggt gatcggctat ccatgcaatg gctttgtcga
    17521 tcttatggga tggtgcgagg gcgttatcca tcatcgcatc atcgatttcc cagttacgtt
    17581 gacagtacac acagatttga gggcatgtat tgaatggttt gagaatgaca atgcctggat
    17641 atcgtcgagt aacgaggtcg atgggcgacg tgtctcgttc gagcatgaag tccaatccgc
    17701 acgatgcgcg gtggtctttt cgcatgctgt cgacatagga gaggggaggt aaaacctgag
    17761 cgcggatagc cctgtctcga acgaaaggtt catcgtccat gagcgacaga taaaaagggg
    17821 tgataccaaa aggcagccgt tgtcgcctaa gacgcgatat cgacaatttt tcctcgtccg
    17881 tcagcgtgac acatcgacca atatcgtctg gatctcgcag tacgtgcgag atctgccaac
    17941 gataatcatt ccagtcgtcg agcgttgccc cgagagcgtc gagcacgcgg tcgcgacgct
    18001 ccacgcgtcg ctgccgtgat tggggatcga gtccggaggt ataacgatcc atcagccacg
    18061 aggcgcgttg gaacagacga tcgagttcat cggagcgggc gatggctgct tcccgtcctt
    18121 cgatggcatg attcatcttc aagtcgttgg ctaagcgtgc cggaagcttt ctttgaactc
    18181 cgcgaaagag gtgagtcaat tcggcgaaaa acgcaggtga tagatcgggt cggtcagagc
    18241 ctcgggcgat gtcaaaaagg gcttgtgtga cgctgaagcc actgagcgag tccgagcgag
    18301 cttccaagat ggatcgaaac gtcctgacgc agtctcgtac gtacacggtt tcgagatgtt
    18361 tgtattcgct atgcgaaaac gtgtcatatt gcagacgtgt gacacgatcg aacagtcgag
    18421 tgcgtgccgt ttcgacatga ttggcatgcc tgatgacagt gagaagggga gtggcttctt
    18481 cgcgaaacgt ggtgatgttg aattctgcgt tccagtcggt gctcatgatt tcctgtctgc
    18541 caatacacgg tgcgaccgat cgatcctttc gtcatgagga gcggaccgaa cacccgcttc
    18601 gggaaagatg atgtcgaacg acctattgtc cttgtccctt gtcatggtgg aaacgaaggg
    18661 tgaaggaccc atggttcgat agacgatgcg atctgcatat catgcgagga cggatcgaaa
    18721 aacatcgagc atgtgtgatg cgtggatttc acaggaaaag cggggagccc aatccagacc
    18781 agcggatatg aggctttctc ttcgcgcagg atcgacgtgg agggaatgaa tggcttgtcc
    18841 gatggcttct gtatcgtcgg ggctttgcac aatcgcagcg ttgcccgcga tttcttcgag
    18901 cgatccgcct cgggtcgtga tgacggggca gccggaagcc atggcttcaa tcacggtgta
    18961 accgaatcct tcgaatcgag aaagcagcag gtgagctagg gcttgtctat acagcgctcg
    19021 cagatccgta tccgagacaa atccgagccg tcgaatacgg ttgtcaactc catggtacga
    19081 agccagtcgt ttgagttctt cgattttgga agcgtccaga tcgcccacga aagcgagaat
    19141 caggtcgctg cccaggcgat gggcgaacgc cagacttttg atcatcccct cggtgttctt
    19201 tcgccagtcg aggtccccgg cgtacaatag gtacttacgg ggcgtgagag caaagcgagc
    19261 aagcctgcgt tgatcatctc cgggatcgtg gggagaaaac gtcgataggt cgacgccatt
    19321 atagacgagc gaaattcgat cgtgagggat atcgagcagc ttgatgagtt gctgtcgagt
    19381 ggcgttgcta atggcgacaa catggtctgc ggaatggaaa cgtcttttga tgagtcgttt
    19441 tccaatgact ccgaacccat ctcgaacgcc taggtattgc ttagggaatt gcagtgggat
    19501 gaggtcgtga caagtcgcca cgcgtttaca ttgagcgaaa gacatcgcaa gcggtgttgc
    19561 gttggggtca ccgaggtgca cgagcctagc gttgagcttt tgcatggctt tccacaaagc
    19621 caagcgcctc gtgtatgccc atcgataacg atcgaactgg gttggagcgc gtttccaggc
    19681 atgagtggca atgcagtgga agtcttccga gatgatgggg actccgatgc cgctgagtcc
    19741 aagcaaacca atcggtcgaa tgtctttcga agcgttgggg tcgagacgcg agaggccaag
    19801 ggcaagggac ctgagataac gcccaattcc gcgtcgttgg gtaggggtat caagagggga
    19861 gaagtcgatg acgacagagg tggacatgag ccaacagcgt agatcaaggt cgaattccaa
    19921 gcacgcgctg atagaccgcg agtgtttcgc gagcttggcg ctcgacagag aattgaatgg
    19981 ctcgttcgat gccccgagcc acgatatccc gtcgaaggtc gtggtcctcg atgaggcgaa
    20041 gcagtgcttt tcctatgtgc tcgtggtcgt cgggttctac gtgtaacgcc gcatcaccgg
    20101 cgacttccgc caacgccgtg cgtcgcgtcg tgatgacagg acagccacag gccatggctt
    20161 ccaccacggt aaggccgaag ccttcgtgcc gagacacgag caagtgcgcg gcggcacaac
    20221 ggtacaggca ggcaagttct tcatctgaga cgaagttgag gaggcgaagg gcatgccgaa
    20281 tgccaaggtt gcgagcttcc cgatcgatcg cggagaactc gtctacactc agtcgtccgg
    20341 cccatacgag ttgcagacca agatcgggct tcaattcccg cgcgtaccgt aaggcccgca
    20401 gcatgccgga ttggtttttt cgccacgaag catgaccgac atagagggca aaaggcgtgt
    20461 tcgagatgcc gtacttttga caaatgcgtg cgtcctcttc gtttttctca ctgctccaac
    20521 ggttggaatc aatgccgtta tacacggtcg aaatccgcga tgaaggtagt ccaaaaaggg
    20581 ttcgaaggtc ctcggcggtg tacgacgaaa tggagatgat gtggtccgcg gaaaggtagc
    20641 ggtgagccat caggcgtttg tgaaccgagg ttgtgaatcg aagccatgga cggttggatc
    20701 ggacggtagc gaggtcatgg caggtaacga gttgcaatgg gcttctggtg aaccgaagtg
    20761 gtggagcgtt ggggtcaggg agatggatga ggtccgcgcg cgcgtttttt gcggcccgct
    20821 gaagtataag cctgcgaaac cattgagttt tctgatcgtc cagcaaggag gctcgtgggg
    20881 gcatggggtc gaattcgata tgctgaagat catgcaaagc gttgtcgttt gcaaagggcg
    20941 acagatgcac gagtcctatg attcgcaaac cgcaatcatc gctttggacg atgcgtgata
    21001 ggccggaggc gagcaattgg acatgtcgtc cgataccgcg aaggcgaagg ctcgtatcga
    21061 gggcagacag gtcaacaagg acggtcttca tgcttcgagg gcatggtagc gcaagcgttg
    21121 ccggaactgg tgcagataca acgcaacacg gggaccgatg tgtccgaagt aaggggaagg
    21181 aggaacgagg aatgtaccat gcggaaggta gtacgacggc aacgatggag attccgcgcg
    21241 ataggcgaag cagcatgtat tggatcgacg ttggcgatgg gaacgtacat cgcagttatg
    21301 cggttgggga gggggtgagg ctggttgtgg gtagtgcgaa agctagtgat atcgtgttga
    21361 tggatcggat ggtgagcgct cggcattgcg agctacggct ggaggacgga gtgctatggg
    21421 ttcgggatct tggctcgaaa aacgggattt acgctggggg ggctcgggtg gattgtgcgc
    21481 gtttgggtga gggatcgtgt ttttcggtgg gtcgggtggc gtgctgtgtg cggtccgaaa
    21541 gcgaatcgat ggagtcatgt ttgccgcttc cgggagtcgt cggtacttcc gcggcgatga
    21601 tgcgagtggc cgcgcaggtg cgacgagtcg cgaagctgtc gattccggtg ttgattcgtg
    21661 gggaaacagg cacgggaaag gaggtgattg ctcagacgat tcacgcattg agtactcgaa
    21721 aagaggggat atttttcggg gtcaacgtgg gggctttgcc gccttcgttg gcggccgctg
    21781 agttatttgg ccatgatcga ggtgcattta ccggcgcggg ccaagttcgc aaaggagcct
    21841 ttcaagcggc ggaaggcggc accttattcc tcgatgagat cgctgaagcc ccgttggatg
    21901 tgcaggtgct tttgttgcgc gcgttggagc gaaaggaagt gcttcccttg ggctcggacc
    21961 ggcatgttgc cgtggatgtg cggatcgtgg ccgctacgtt ggctgggttg gaagaagccg
    22021 tggccaagcg ccgttttcgt gaggatttgt tgcatcgctt ggcggtcgga accgttcagc
    22081 ttccgagcct tcgagagcgc aaatccgata tcgggatatt ggccgaacac tttctgtggc
    22141 aacgcagaga tgaagtggga gtcaaacgac tcactccagg cgccatgggg agactcatgg
    22201 tccacgactg gcccggcaat gtacgagaat tgtcgaatat cgtctttcgt gccgcggcgc
    22261 agacagacgg cgagtgggtg cgagcgcgcg atgtagatgt ctgccttgcc gctcgtcgca
    22321 ccacggtggg acccctgtct cccacagccg cggaggcgtt ggtgatagcc tgcagaggca
    22381 atatttcgat tgctgcaagg aagtgcaacg tggcgcgatc gacattcaga ggctggttga
    22441 atgcagggaa aagtgatcga aagaagggaa agtggccttc ggaaagacag gagtgatgcg
    22501 gcaggagagc gggtgacaga cacttcgggg gggatggtcg atctggatcg atgggtatcg
    22561 tcagaaggtt cccccgatcg acagcccctg ataatcggag ctgatcaaag ggaggatggt
    22621 tgtggagcga accgcggaag acgtagatgc cgcggagtcc tcgggttccg tgcgtagata
    22681 atacaaaacg cctgtgctga tggctcctac gccggcgacg acgaagccga tggttgcgac
    22741 ggttttgtcc ctatctctga tgtccaggtt gtccttgagc tggcgacagg cagggccata
    22801 gttggtgcga gaagtaatcg gaacgggatc ggcgcatggg tttttttggc gatccccgag
    22861 ttcgtcgtct cggcttgcaa cgagcttgat ttggcttgcg atggagtcgg cgtcgttcga
    22921 ggcgctatgc gaaaagatag caaacaggat gccggtacca agacccagga ccgtggctcc
    22981 tcccgaggcc cacgcgactt ggtcggaaat caaccagtcg aaaaaaggca tacgaccctc
    23041 ctgcttcgtg gattccggcg gctcggatga ttccgtttcc ggagcgccga ccggtggtgt
    23101 agctgagagg tcgaaagaga acataaccgt ccttacttgg ccgatcggcg catcgacggt
    23161 cttggattgt ggaggtttgc ctcctgctcg aagttctacg gttcgtgacc cgggggcaac
    23221 gtccacatca tcaaaaagag gggtacgccc aatcaaagtt ccatcgacga atacatccac
    23281 gccatccaaa tttgctttta tatcgagtct ggcggttttc gtacgcgcgg ccgcgagccc
    23341 ttgctctgcc gcggcgcgtt cctcggcttc cacattggga ttgtctcgaa ggaattcgga
    23401 gaagtggcga gcggcttcga caggatgatc ggaatgcagt tcgctttgcg cgagattgag
    23461 caacacggcc ggatgctgtt tgagggcgta agcctgaagg aaagcagccc gcgcttcttc
    23521 gaatttgcct tgatcgaata gccgaactcc ctcctggaat cgctggcgcg ccgagtcggt
    23581 gaatgcagtg tccgccgtct gagccatggc gttcggaatg gagccatgga cgggcatcga
    23641 cagcgcaacc aacaagaaag cacttgcacg aattctcatg ggtggccttt cgaaaatagc
    23701 atgagaaggt tgtcagaagg gtgtctcgcg aacaatcacc ggtggcgctt tggtaccggg
    23761 tgaaggtgcc gaagtttttg gcggttttgg cggtttcggt tccggtgcaa ccgtcgccgt
    23821 cgtaacgggt ttgggctctc ggtctgggtc cttgtctttc ggttttggtg gggcgtccac
    23881 tgtcgttggc gctgacggtt gtttggtggg aggttggcgc gtatcggtgg agactaccgg
    23941 ctctgggggc ggtgcgatcg gtggttccac cgtaggcgtg ggctcttgga ttgacgtgga
    24001 gcccacgggt tcctgtgcga tcggcaatgt gtccaccgaa gtggcggact ccgcaggtcg
    24061 cgacgaagtc gtggactggg tgggttcgac agggtcggag gaccatagct tgatgccgcc
    24121 cacaacgatg gctacggcag cgacggcaac caccgcaatg acggcaacag gggtgcggcg
    24181 ttgcgatggt tgtagatccg gttgcgcctc gctttctgca gtcttgggag aagcatcggc
    24241 atcgaaagga tcgtcgtcag ggacaacgat tgacggttca tctttgatga cgatttcggt
    24301 tcgttttgga atggcggcga tgatgtcgac ggggccttgg tcatcatcga ttcgttcgtc
    24361 tgcgggtggt tcgggatgag gcgaagtcac agcagattcg ggtggctgtg ttttgtcctc
    24421 gtcagccgaa accacttgct catcgattgg ctcgtgggtg atgtcggcag gcggcgatgc
    24481 atcgtcgatc gacgtctccc acgcgggttt gaattgagaa gcagcacgct cggcttcctc
    24541 ggcggatagc ggtgggacgc tgtcgcgagg ggccggaacg tcttgttcat ccggaattga
    24601 tttgggaatt gacccactgg tagcttcgga cattcttcgc ctattcgcgc ccgtcagtta
    24661 gattgacagg aaacgtagca tcccaacaat cgtgctatca acggtagtgt acggtatggg
    24721 agcaaacgaa ccgtgagcat tcaagagtaa gatgtcgtga acagcaaaaa tgtggcgcag
    24781 gacagacgtc gatggttggg ctcccacgat ggcatgcgct cggtggcgca agcgagaggt
    24841 tggtgggcgg cggctctgat gatggtattg aacggccttg gtgttggctg tagcagtgaa
    24901 aaaacagagg aaccctccgc gctttctatc gtcgacgcat cgaggatgga ggcagatatt
    24961 cagggatttc ccgaatatga gcacgccaac aacatcatgg ctagcgacca tccgttcaca
    25021 tcgagagcgc aagccgtaac gatagcggat cagctttcgc tggcgctgga agggaaaaaa
    25081 ccgcctcaaa ccctattagg ggccgaattg gtgggggatt tgaggcgtcg ggcctatcga
    25141 acgtttcacc aacccgcgga tgctcgtgaa gcgatccttc aatacgatat ggtaactcgc
    25201 gggcaagacg cgcagcgcgg gtgcatttca cgccttgcat cgcttgcgtt gcgcggggaa
    25261 attgaggata atctagatag tatttattta tcaatctatg aagaaaccaa gaagcaacta
    25321 cccgtgccct gtgagggtct tgcgcgacga gctttggagg atctacgagc gtatcgtcca
    25381 gatgcatcgc gactcgcgga aatcgatcgt cggtttcacc aggcccaggt ggatggggga
    25441 gcaggtaagg aagctgtagt caaaccaggc ccagagacgc gagctggtcc gtcgaagata
    25501 gaatccattg aggtattcag ctctcgggat gcggcgcggg tcgtgattca gcttacggcg
    25561 ccggctttgt atgaggttgt gtcgttaggc gcggagggag cgcaggggcc gagggtctat
    25621 gtggatttgc agaagaccaa gcgagggcag gcgcctcggg agcggcgagc cgaagggttg
    25681 gtgacggcgg tgcggcttgg gactcaccag gacaagacgc gagtggtgct ggatttgagt
    25741 cagcaggcgt tcaaaagggt attttacatg ccggagccgt ttcggatcgt gttggatatc
    25801 agcacgacgg caccgatatc ggagagggcg aaaggggaga cggggcctcg tcaagtgcga
    25861 agggtggtat tggatcctgg ccacgggggc agtgatccag gggcgattgg gcctgggggg
    25921 ttgaaggaaa aggatgtgac gttggatata gcgcatcggg tagcgccgat attgtcgcgg
    25981 gagatgggga tcgtgacgat gttgacgcgc gatgatgatc ggtatgtggc gttggaggag
    26041 cgggcggctc gcgccaacgc gttccatgcg gatttatttg tgtcgattca ttgcaatgcg
    26101 gcggaagatc cgagccatcg aggggttcag accttcgtct tggctggtgc caaggatgag
    26161 gcatcgcgac ggttggcggc tcgcgaaaat gcggcatcga tggcggcggg gcagcagatg
    26221 ggagcggtgt tggacgaact gcatctggag tcggtttctc atcagtcctg gcaattcgcg
    26281 actttgttgc agcgcgctac gttatcttcg ctttcggaga gttatggggc ggtgccggat
    26341 gggggggttc gttccgcggc gtttttcgta ttggtgggag cgcagatgcc ggcagctctc
    26401 ttcgaggtgt cttttgtgtc caacccgcaa gaagagacgc gattggcgac ggtggattat
    26461 cggcaaaagc ttgcggatgg ggttgtgaac gcgatccgtg cctatcgaga tggccgttga
    26521 tcttggactc ctcaagttcg gtcgagaccc atcagacgct cgagggagtc tcgtaattcg
    26581 gagacttctc ctttgagaat gaaatcgtag gcgcctgctt ccaacgctgg tttgcgcata
    26641 tcttcatggc ccgagtacac gatcgaacga atatgcgcgg tggcttcaat ggacttgagc
    26701 cgttcgagca accgtagtcc gtcgagaccc gatgttttga catccaaaat gatggcgtcg
    26761 ggctgaaggt ttccgaccgc gacgagagca tcgaaagagt cctggaaggt ggtcaactcg
    26821 aagcgtcggg tgagcacccg acgaagagct gcaaggacat tggcgtcttc atcgactgcg
    26881 acgacttttg gttttccaag acgaaggact tccgggattg gatatccata ttttcgaaga
    26941 aaatccagga cgtcgagcct tcggaagcgc aagtgccgcc cgggggtacg gaagtgccgg
    27001 atctcccctt tgtcggccca gttgtggatg gttttcaggt ccacctggca gaagcgggcc
    27061 acgtcgctcg cggtgaagag ttcaggatta ttgtggtctt tggcgtccat gggtgaattc
    27121 tctttcctgg gtctatcttc ggatcgtgtt cgattaacca tgagaaaaac gaggaatcgc
    27181 ggcttcctga tggttatgtc caccctagca gaaacgccta aagaatgctt caaaattgaa
    27241 agggaaagaa actaattttt ccggtttatt ataaattgtc caaggcaata ctcgaagtaa
    27301 gtgggttcaa cgacaaccct agggcgctgg ctatgtcccg gatgccctgg tagtacaagt
    27361 aaggttctat cgttattttg gaaaatttcc gggataacga tgtgcagtgg cagtttggat
    27421 atcgcctcga agaatttgtc taaactattg aatttgctaa tataaaatag catccattgc
    27481 gtgggcggca ggatttccag ttcgtgacgt tcaaactgat ccaggatctc gtcggggaac
    27541 gcccaaaaac agtccatatt ctccgttccc actcgaagta tctcttgtcc caagggcatc
    27601 cgcatcgcaa aaaaccgagt gtcgaaacgt accggcagca tcggcggcgt cacccatcgc
    27661 gacaccggca cgagctgtga taatcgtagc tcaatggctg aagatgcgag cagcgtccac
    27721 aaccgccccg gctcatagcg caaacgtagc ttttggttcc agctccgaga cccgggagcc
    27781 gatggcatct cgacctcatc ggtgggcagc aacgccgttt cttcgaatag ctcgcgagcc
    27841 gcggcgacag cgcaagcgcg ttcggtcgtg ggatcgtcag aaaactcaaa aattcgtgga
    27901 tgaacggcat cggcgggcgc ggtagcatcc tccggatcga ccttaccgcc cggaaaagcg
    27961 accacgccgc cccagacgcg ctggttaggg tgacgacgca cgcacaacac ttgtaggctg
    28021 ccctttcgtt cacgaacgac gagcacggta gcggccaaag gcggcttggg ctcccccatg
    28081 ggggtgatgt ctccagattg tttggttttt tttatcatgg gaagagcggc tggtcggccg
    28141 ttgtgatgga caatgattga cgttacgttg gttgttatct acaatacccc gcccttcgcg
    28201 accttgatag catgtcgtga cggcggagat tcaggggtag ccgaggatga acaaggccca
    28261 actcaaaaaa ttccgagccc tgctcgagca aaagcgtgaa gagctggttc gcaaagccaa
    28321 gcaaaccctc gatgaagaca tgacgctcga tgtgagcgat ctgcccgacg agatggatct
    28381 tgcttcgagc gaataccttc aatcctttac gtttcgtctg cgaggccggg aaaagtcgtt
    28441 cctcgacaag atcaacaagg ccctcgccaa gatcgacgat ggatcgttcg gtgtttgcga
    28501 ggagtgtggg gatgaaatct cggtgaaacg gctagaagcg cggcccgaga ccacgttgtg
    28561 cattcgttgc aaagaggatc aggagcgtct cgaaaaagac tttggttgac gcttcacctc
    28621 ggttcaatcc cccttctccg ttcaccaacc ccacggggac gcagcctgtt gattccttgc
    28681 cgtttcgtca tgtccccggt cgatgctgcc gccctttcga agcaaccgct tccgttttgt
    28741 gttttcgact aggcgctgcc tgttcttcga gcacacgggc aaggtagggt gccgtttgcg
    28801 acgtttcgca ttgtgctatc cgatgaggcg ttcccgtggc caccagatgg ccaccgcgat
    28861 ctccgccttc tggccctagg tcgatcaccc aatctgaaca cgccacgatg tcgaggttgt
    28921 gttcaatgat gacgacggta ttgcccgcat cacgcaaagc caacaacgct tccaccagca
    28981 ccgtgatatc cgaaaagtgt aaacccgtcg ttggctcatc caatacatac aacgtccgcc
    29041 cggtagcgcg ccttgccagt tctcgtgcga gtttgacacg ctgagcttcg ccccccgaca
    29101 gcgtcgtaga cgattgcccg agttgcaggt agcccagccc cacccgcgat agagccagca
    29161 aacgctctcg aatccgaggt acggcttcga atacctccac ggcgcgctcc accgtcagat
    29221 ccagcgcatc cgcaatggaa agaccacggt actgcacctc gagggtttcc cggttgtaac
    29281 gtcgtccccc gcaggtctcg cagcgaacga aaatatctgg caaaaagtgc atttcaacgc
    29341 gaagcacacc atcgccctgg catgcctcgc atcgtccgcc cttgacattg aacgaaaaac
    29401 gtccggcctt gtagcctcga gcgcgcgctt ctggcaatgc cgcgtatagc tcccgcagca
    29461 gcgtgaacac gccggtatac gtcgcgggat tggagcgggg actgcgtcca atgggcgcct
    29521 ggtcgatggc aatgaccttg tcgatatgcg aaagcccttc gattcggtcg caagaaccga
    29581 cagtgctgca agcacggtac aagttggagc gtaccgcttg aagcaatgta tcgatgacga
    29641 gcgtggactt accggaacca ctcacgcctg tgactgctgt catcaatccg atggggaatt
    29701 cgaccgtgat atgtttcagg ttatgcgcat gcgcgttgac gatacgcagc acggattggc
    29761 ttggttttct gcgtttggca ggcagaggaa tgcgtttttg gcctgacagg taagggccag
    29821 tcaccgagtc agggcattgt tgaatttgtg agggactgcc ttccgccacg attttgccac
    29881 catgcacacc cgcggccggt cccatgtcga tgacgtgatc ggcggcggcg atggtatcgc
    29941 ggtcatgttc cacgacgatg acggagttgc ccaagcgtac caaatagcgc agagcatcga
    30001 ggagtttggc gttatctcgg gcgtgcagtc caatggacgg ttcatccagc acgtacagta
    30061 cgcctacgag tgcggcgccg atttgtgtag ccagacgaat tcgttgacct tcaccccctg
    30121 acaggctttg cgtggcacgg tcgaggcata aataatccag cccgacatcg atgaggaagc
    30181 cgaggcgagc gatgacggcg cggagcaaag gttcggcgat agctcgttct cgatgagaaa
    30241 gaccagagtc cttcgccaat aattcttgca atacggttcg tagattgccc aacgacagct
    30301 caccatactc cgcgatattt cgattcccta cgcgaaccgc aagcgcttcc cgtcggagtc
    30361 ttcgtccatg acaagcatca caggtgctgg tggccgcgaa gtgggaagga tcgacttccg
    30421 ccgtatcgtc atccagatcg gaatcatctt ccgatggctc ttcatccaca ccggcgaggc
    30481 gtcgttcgag gcgcggaacg atcccttcat aatcgctttt tcgcctggtt gttttgggta
    30541 tcccgaaaag gatagcgtgt ttggtggact cctccaactc gcgaaacgcc aggttgggat
    30601 tgactttgag agcgcgcacc gccttggcta cttcggtggc caaggcgagg cttccccgtg
    30661 gcccccacgc gaccacggca ccttcccgca agctcaagga atcgtcggga acgattcgat
    30721 ccgggtcgat acgggtgcgc gaaccaagtc ctccacatac gggacaggct ccgtggggac
    30781 cgttgaacga gaacattcgc ggttcgatcg cgggaaaaga aacatcgcaa tcgatgcaag
    30841 caaaccgctc gcttttccag atggggtccg cgccagacat atcatcgatg agaagacgcc
    30901 cttcgcctag ttcgagcgcc aattcgaccg aatccgtcac gcgtcctttg attccgtctc
    30961 gcacggcgat acggtccacc acgacgtcca ggtcgtgcgg ttgtgccggg tcgatgcgaa
    31021 gttcctcacc gatgtcgatg atggttccgt cgatgcgtgc gcgaacgaag ccctctcgac
    31081 gaagacggtt gatttcttcg tgcaggtcgg agcgcagacc tcggcacaag ggagcgagca
    31141 gaatgatgcg tgatccttcg gaaagggcta ggagtccatc gacgatttgt tgaacggtgt
    31201 gggcttcgat gcgtttgccg caggtggggc aatggggggt accggcacgc gcgaaaagca
    31261 ggcgcaaata atcggcgatc tccgtgacgg taccgaccgt cgagcgtgga tttttaccga
    31321 gggctcgttg ttcgatcgcg atggctggag acagtccttc gatggcatcg acggctggct
    31381 tggcgagttg ctctaggaat tgtcgagcgt aggcggaaag cgattcgacg tatcgtcgct
    31441 gtccctcggc gtaaatcgta tcgaaggcga gggaggactt accggagccg ctggggccgg
    31501 taatgacgac gagctgattt cgagggatat cacagcttac gtctttcagg ttatgctgtc
    31561 gcgcgccgcg tacgacgagc ggatgcatgg gcagcctcac gggtttgggg gaaaggaatt
    31621 gactagcaca ttcaaggggg tggaaagaag cggggagtgg gattacctgt tcgggcgcgc
    31681 aggtgtccga tgtgttttct tacattttgc ggcgctcatg gcttcccctt gaagcagggt
    31741 tcgactagct tgcctcgcgt ggttgaaacc acgaacccgc cccaagtttt ctaccttgcg
    31801 catccgccct acacctgcga tttggctgca gggacgaggg tgcgaagggc ccatagctca
    31861 attggtgaga gcccccggct cataaccggg ttgttcctgg ttcgatccca ggtgggccca
    31921 cgattgccaa ccatgtcgtt ctcgtacagg agctatcgtc gaacgtgagc attcaagaac
    31981 atatcgttta ccttgaggaa ttgtcggcac tggatgagga aattcgggcc gtcgacgata
    32041 cattgagcac cgagcgcagc gcgttggcgg ggaagaaaga agaacttgcc cgcctcgagg
    32101 agaaaatcgc ttccgatgag aaaagtatcg ctgaaatggc gaaaactcgt acggatctgg
    32161 tgttggaagt cagacaagtg acagccctgg tggaaaaaag tcgcgaaaaa ctatctcgag
    32221 cgcgcaatga gcgcgaaacg aatgcggcga cccgagaact cgaggaactc cggaacattc
    32281 aaaaagggca cgaagagcag attggcaagc ttcagctcct cgaaacggaa gcatcgaaaa
    32341 gcaaggaaga ggctgaagta gcgcgacgcg ccttggagga ccagcttggc acgaccgaag
    32401 gggtggtatc gaaacgtcta gcagaagcgg aacaacagaa agcgcagatg ctcgaacggc
    32461 gtcaaggcgt ggtcacgaag cttccccggc cgattctgag ccgttatgag atgattcgta
    32521 aacgacgggg aatcgccatt gccgctacgc ataacggcat ttgtgaggct tgccacctgg
    32581 ctgctcctcc gcagttattt caaaagatcc ttcgacagga aaccttcgaa gtgtgtcctc
    32641 attgccaacg aattctgtac ttcaaaccgt ccgacgctga gtcgaacaag gaagagcggt
    32701 aggcccggtt gtgaagagtt gcccagtatg ccgacgcctt tttccagatg atgcagggtt
    32761 ttgccccgtc gatggtgagg ctttgttgtc ggcggctgag gtgcagcccg cggtggatcc
    32821 ggacgacgcg cgtgtcggcc aacttctcgc taaccgctat caagtccggc ggattgttgc
    32881 cgatggcggc atgggacgtg tttacgaggc gctggacttg caagccgagc gccgggtagc
    32941 cctcaaaatt ctgcaccccg ccgtcgtgcg ggaccgagtg gctgtcgagc gtttcaagcg
    33001 ggaatacgag atctcgaaac tgttgccgca cgagcatatc gtcgaggtgt tcgactattt
    33061 cgctttggat gacggtactt acgctctggc catggatttt ctcgaaggcg aagagcttcg
    33121 ctcgctcttg aagcgggaga agacggtgcg ccccgcgaga ctgattcgaa tgctcagcca
    33181 aatcgccatt ggtttggacg aagctcatca acgccaaatc attcatcgag acatcaaacc
    33241 ggataacgta ttcgtctgtg gaacgcgtga aggggacatc atcaagctac tggatttcgg
    33301 ctcggtcaaa gacaagtccg aaggggcgaa gaagttgacc gtgatgggca ccacgattgg
    33361 ctccccctat tatatgtcgc cagagcaggc gcagggattg gacacactcg atgcgcgagc
    33421 ggacgtatgg gctctggcgg cgatctcgta tgaagtgctc acgcagcagg tcccgttccc
    33481 tggaaaaaat ggcaccgcca ttttgctagc catcctgacc caagaaccga tacccgcttc
    33541 ccgtgccgcc cgtgatttca aagaggcata ccccatccct cctaccgtcg atgccgtgct
    33601 caaagaggct ttcgccaaag accccatcat gcgggtggct tccgtcggac tgctggccga
    33661 caaacttgga catgcattcg ggctcgaagg gatgcatcga gattgggcct atgcggacga
    33721 actggatctt cacgctcgga tcgagtcggt cttgcccgag ttgatgcgcg ctgcaccgga
    33781 accggagcca cttcccacga cgcccgtggc caccgtagaa gccgacccat ttgcgttgac
    33841 gacctcggat gcgaagccat ctccagcagg agaaaaagcc gggtcgagca ccgaggagct
    33901 tcgcgccgtc gtagtcccaa agcgcttgcc cattgccttg atcgttgggc ttttcgtggg
    33961 gggattgatg gtgctgctcg tcggtgcggt gcttgtcatc ttgtggttga gatgagcctt
    34021 tcgtggagga gcaggggtta tccatgaaag caaagagttt ttcgttgttg gaccagtggg
    34081 gcaatcgtcg aactctcgag cagttcgagg ggacgtacgt ggttgtctac ttctatccga
    34141 aggatttcac ttcgggctgt acgaaggaag cggaagcctt tcgagatgcg gctgaacgat
    34201 tcgaggcttt gggagcgaag atcgtgggta tcagcaaaga ttctgtcgaa tcccacgcaa
    34261 aattcgcgaa caaattgggg ctgtcgttcc cgttgctctc cgacccggac cttggggtca
    34321 ccgaacgcta tggggccatc ggccccaaaa atatgtacgg caaaatcgtg caaggcgtgc
    34381 tgcgttccac gtttctcatc ggtcccgatg gcacgttgct tcgttcgtgg aatcgagtgc
    34441 gggtgccagg gcacatcgaa gccgtcctag aggcgttggc tcggtataaa acatcataaa
    34501 atcaatatgt tatcgaactt ttacaccgcc gagttcaggc tctcccacgc cgattcgtcc
    34561 ccataaaaaa gcgacacggc gacgatactt gcccaaacca tcgcctccgt acacccagag
    34621 gactcgtcgg tcgtcgtcga gttgaacgtt gggtagccac catccgcttg gggcgaaagc
    34681 aagggtttgg ggctggtggt tttggattcg catcaggacg acgccgcata gagcatgggc
    34741 cgttggatgg gcgcagccgc tgccgagcac gatttcttcg ttgccgatgg gataccactt
    34801 ccagatgcga tcgatgcgaa agggtttcgt ttcggagagt tgttgggtga ttttttccgg
    34861 gaggccttgt tccaacaggg aaaccagttc gggtgtgcga gccacggctt tgagggggtc
    34921 ggaaggacga ctaacttcca aagaaacctc ggcatcgttt ggttctcctg cgcagcaacg
    34981 aaaaccaacg tctgcgtaag acttatcggg acgtcgtgct cgtgcgttgg cgcatcgacc
    35041 gacgacttct gctggttcgc cggagcctcc gcgaacgacg gctagggtgg atgattcgcc
    35101 acggttccaa gggctcgagg tccattcgaa cgcgccaccg tgcaagtccg cgacatcgaa
    35161 tccgcttcgg caaccggcgc tgctgccggt gggagccatg gtgcccgttt tgctcatggc
    35221 gcagatcgac ggattgtagg cggagccgta ttcgtatgtg gtggagtttg gtcctttgca
    35281 agcggcttcc cattcgagtt cggtgcagag gcgtttgtcg atggcttcgc aacgggcttt
    35341 ggcttcgtct cgcgtgatat tcgtggtttg gatggcgccc ggttcattgg ggaaagcgta
    35401 ttgatcgata tagaaaccgt gcaacacgac ctgcacaccg ggcatttctt cgtcggcgat
    35461 acgaggcaga acatcggtgg gtgtgccagc tacgaatgct cctggaggaa tccacaccat
    35521 tcgtttgcgg ggcacgggtg gtcgttcggg ttctggcaaa gcgacgacga gggcgtctgg
    35581 atgatcggtg gaggagctat tggaacagga tcgaggggag catcccaaag ccaggcatac
    35641 tatcaaggtc gagcctatcg gtatgtcgaa cggctggctc acgcgtctgg tttctcctct
    35701 cgatcccgga gaccgagttc tttcattttg agttggagcc cttttcgaga gattttgaga
    35761 attcgggctg catgcgtgac attgtaggcg gtttgttcga gggctcgaac gatgagttcc
    35821 cgttccacgc gtgttcgtgc ggcccgcact tgttctttga gtccatcgct cgacaagacg
    35881 gcgtcttcga tggagactga cacgacgggt gggcaggatg gggtttgtgt ttctcgcact
    35941 tcgggggata gatccgcggg agtgatgttg gtgccatcac agaacaagac ggctcgttcg
    36001 atgacgtttt cgagttcccg gatgttgcct ggccaactgt attgtcgaag caattcgagg
    36061 gattcttttt cgactcccgc cacggatttg ttgagccgtt cgttgaattt ctggatgaag
    36121 tgttgggtca acaggggaat gtcctcacga cgatgtcgca aggagggaag ttcgatgggc
    36181 acgacgttga ggcgataaaa aaggtcttcg cggaatgcac cggcggcgat ttcctttttc
    36241 aagtcccggt tggtggaagc gacgaggcga acgtcgactt gaatggtttt gattccgccg
    36301 acgcgttcga actcgctttc ttgtagaacg cgcaggagtt tcacttgcat ttcgatggga
    36361 atttctccga tttcgtcgag aaatagggtt cccgtgctcg cgagttcgaa tcgtccaggc
    36421 tttgcgttga ccgcgccggt gaatgcgccg cgttcatacc cgaaaagctc cgattccatc
    36481 aggtctttcg gaatggcggc gcaattgact ttgatgaacg gtttgtcacg gcgagaggaa
    36541 ttttcatgca gcgcgcgagc cacgagttct tttcctgttc cactttcgcc ggtgatgagc
    36601 acggtggtcg gcgtatcggc cacccgttcg atgaccgagt acatttcttg aagcggcttg
    36661 ctgtcaccga tgatcccgaa tttggcacct tccacatgcg ccctggcatc cagcccaagc
    36721 tcggcattgg cgaggtttcg tgtacgcaat cctttggcga cgatggcgcg cagttcggtt
    36781 tggtcgaaag gtttggtgat gtagtcgaag gctccggttt tcaaagcttc gaccgcattg
    36841 tcgacggttc cgtgcgcggt gagcatcacc actggcaggt ccggatcgat ttcgatggca
    36901 tgacgcaaaa gggccattcc atccattttg ggcatacgca gatcggtgat caccaggtcg
    36961 atatggtgtt cgcgaaggat ggcaagcgcc cgctcaccgt cttccgccgt atgcacttca
    37021 tagccttcgc gcgcgagttg ggcagacagg acgcgacgga tgttggcttc gtcatcgacg
    37081 accaggactt ggtctttgga tggcagcatc atatcacctg gcagggttat tcgcagcgtg
    37141 aggagtcatg accagcgccg cccgtggtgg agcagaaaaa gatgaaaaac gagcctagac
    37201 cgggagcgcc tgccacgacg acatcgcggg agccgatgcg catcgcggct acggagctac
    37261 ccaaaccatc gccagagtct ctcgaggata tgattcgagt ttcgagcaaa cgggtttgtt
    37321 tgccttccgg atcgacatgg tacacgagcg cagctcccgc ggtgttggtc ccatccacgt
    37381 caagggtagg aaccccgatc accaattcct tggagccatc accattgaca tccgcaatcg
    37441 ccaaggcacg tccgaactcg ttggtgattc tcgaacatcc ggagacagaa gcggtttcga
    37501 cgcaattgac accggtgatg aagccggtgg agccgagagg tgccccactg caatccaccg
    37561 atggcgggta cttggccagg tcatcagcgt cataaatgct gatggtgacg ccgtcggaaa
    37621 tgaaaagctg ttcggacgaa ccctcgaaaa gcttcccgga acgcatgtgg ataccgaagt
    37681 tgtgacgaaa tccgggcagg cagctcatcg gggcgggaac atcgggatgg ggaccatcgt
    37741 gaacgacttt tccctccacg atgcggtaca gccagacgcg tccttgggta tcattgccaa
    37801 taccgtgagg tgaagcgatg gcaagaaggt atccaccttc ggatagtttg acgaccgcca
    37861 cttcgctacc aaacgtgtca ccgaaagtaa ggaatgggtc ttcgagggga agacctggtg
    37921 cggccacggg cgacagcaag atgggaacgg tcgtggctgg agcatagaac catgctgctc
    37981 ccagatgtct cgtggcagcg gcgatgagtg ggacgtcatc cttcgaggcg gcgattttga
    38041 agtcaccgat cagtagctta tcgagagact gcaacgccaa gtcttccgca atgacatctc
    38101 gaggagattt cacggtcggg tgagcttcat cacgaccggg aaccggcaat acgaaatcaa
    38161 gaccatcgct gcaatgaccg agcaatccga gagtatcgtc acggtcgccg acgcccaaaa
    38221 gaaaacaagc ctcatgcggt tgaccgtcga tgagcatcgt ttgcaacgcg gcgggcgtgc
    38281 tgccgaatag acatcgaccg gttggacaga aaccggaagc attgggtgtc gtggcgggcg
    38341 tgtcgttggt gattcggtat gttgcgcctt ttggcgacac attgggttcg cccagcacca
    38401 aaagatgcgt tcccacctcg gtggttacgg tagccatcgt agttccgaat cccacgattc
    38461 ctccagggtt ttccatttca acgaccggag gatgatcttt gagatcgtcg aagcgcgacc
    38521 accacgagca actcgtacag gtggtgatga ggagcagggg cagcgcttgt cggaacatca
    38581 gaacctccct tggagcatga gtccgacacc ctgccatgga tacgaaggca cgatggcgag
    38641 ggcagggggg gccacagggc ttgtcgttcg tggtgccgtt tttcccgctg gagcagggga
    38701 acgcggggcc gaaaggcgag gtggcgcagt atccgaatcc ggatagtcgt ggggcacgcc
    38761 cactcgaatg gccgaggagg gagttgggtc tttgatgaag ttgtacgtgg ataacagcgc
    38821 aagggttccg ccaaccccaa aacaggcagc agacccatac gcgtagattt ttcctttcgt
    38881 gtaccgttcg tcgctttccg tcagcgaacc gttcgtcttg gcggtatgga tgtcgtcgta
    38941 gacgatgtcg gattggactt tgagagcgat gccgcctccg acgaagaccg cggagacgat
    39001 ggcttgtgtc caagcggcgc cgcgaggcgt ggtggggacc atgacgagat cgatgggtac
    39061 gacttgtcct cgggggacat ctacctgccc gacgagatct ttacggtcgt cggcggtggc
    39121 tgtgatttcg tgtttccccg ctggaagttc gatggtgatt gccgagttgg ggtggaaagt
    39181 cccgacgggg ataccatcga ccgacacatg gatgggggat gcattgccgc tgatgtgaag
    39241 tgtgcctttg gagcctcgaa tcaactcgac gagttcttcg cgttcttcac cgtgcttgac
    39301 ggagaaggcg cgggaggcgg aagcgacgct tataacctcg gcagcgaaaa cggcttctcc
    39361 gtgctcgaga tcatacgcgc agccgagcag gccacgggca gggcgatcca ttaccgcgtc
    39421 gccgcgcgcc gtcccggagt attcagcaag gtaggattgg gaacattcgt
//
LOCUS       DAOSOR010000002        37559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_1138_length_37559_cov_4.912169, whole genome shotgun sequence.
ACCESSION   DAOSOR010000002 DAOSOR010000000
VERSION     DAOSOR010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 37559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37559
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1138_length_37559_cov_4.912169"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="PK710_00130"
     CDS             complement(<1..829)
                     /locus_tag="PK710_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08136.1"
                     /translation="MTVEVRETRLGGNLRPFLNVVDTIYRDDPCYVRPLDMDVRDRLS
                     LRNPFFNHGEGVVLTAFRRGKCVGRCTAQIDREHLARYNDDCGFFGFFDTVDDEQVAK
                     ALLDHAGRWLRARQMKKMRGPMSLCVNEELGCLVEGFETPPFFMMPHHRPYQGKLIEA
                     AGLSKVKDLFAWHYTIGQMPERALKAHAAIAQMPEITSRMADMKHVERDIRIVMDVYN
                     DAWSSNWGFVRLTEPELAKMASDMKLILIPELTRIVELDGEPVGVALALPNLNPMIGD
                     "
     gene            complement(922..2298)
                     /gene="mgtE"
                     /locus_tag="PK710_00135"
     CDS             complement(922..2298)
                     /gene="mgtE"
                     /locus_tag="PK710_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOT08137.1"
                     /translation="MRLAHLIGPEIHDLLRDNPEDVRELLDEIHPEDLADVVLELDDD
                     EAGALLQRLAPDAAADIFERLPEGRQEALVNKIGLLKAARIATEMSADDRADLFSALP
                     DNVGEKLLETLEKYDPEAAEEVEELTRWPETSAGHLMTTDYIETHRDATAEDAIKEVR
                     RQGEDVELIHYVYVVDPQDRLIGIASLRDILLADPTERIDEIMVENVISVTPETDQEQ
                     VAKLMAKYDMQAVPVVDGRGEFHGIITIDDIVDVMTEEQTEDVHKLGGVQPIENPYFD
                     TTTWTFIKKRGVWLMVLFLGEMFTGTALRHFDHVIESVAQLSYYVPLLISTGGNSGGQ
                     SSTLIIRGLAVGEIQMGDWYRVLVREMVQGVVLGLILALVGAVRVLLWGDGWNFAVLI
                     GAALVAISIMGCTIGGMLPLMIKRVGLDPATSSTPFIASLVDVFGILIYFGLAQVILA
                     RVIAQAGH"
     gene            complement(2506..3609)
                     /locus_tag="PK710_00140"
     CDS             complement(2506..3609)
                     /locus_tag="PK710_00140"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT08138.1"
                     /translation="MGNDPKLRMVAEKRVGQMLCGKWHLDKLLDIGGMAAVYEATHRN
                     GNRVAVKVLHPIFPKASSVRERFLREGYLANRVNHPGAVTVLDDDTAEDGAVFLVMEL
                     LEGQSMEALLQRSGNVLSVQHVLAIADQTLDVLATAHGAGVIHRDIKPGNIFLTKQGQ
                     VKVLDFGLARLKEADPKTQLTGVGVVMGTSSYMAPEQARSQWDRVDGRTDLFAVGAVM
                     FRSLAGRVVHEAETPTDRLLAAMTKPAISVGEVAPHLPKVVVGVIDRALKFSMDDRWN
                     DAKAMQLAVRQSFALMTAHGQLSTPSSAPPPSESTSDSIVVDMTSEVHELGVEDMMSI
                     APQLMDRLPEVSAYLVEEMLTEADRLDPSDNGK"
     gene            complement(3672..4547)
                     /locus_tag="PK710_00145"
     CDS             complement(3672..4547)
                     /locus_tag="PK710_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08139.1"
                     /translation="MQYQTRTTTREDGFDTAQGYVRDGLASLRNLENLLKSPKIGPRA
                     LEKAVAQIHAGCSPFRDALVSIVDMVGSRKAELPVGSLSDFARDRVDQLCHAVSNAAK
                     KDMGAKSRLRLERQITEVLPDLDVVRELIDLLDSATYSAPTELDLNALLWETLTHRVS
                     PHPNQPEVVPVALSRAAEGATVWTDARVLMPMMSIAFGSVAHQGAKQMHVSAELDEQG
                     GASVLCRRDDDHPAANLPCVWPRVVMPSRDVLSVASALTGVTLSMEPTTVTLRILPQR
                     SADDLGMAVSNRFLS"
     gene            complement(4598..5017)
                     /gene="ndk"
                     /locus_tag="PK710_00150"
     CDS             complement(4598..5017)
                     /gene="ndk"
                     /locus_tag="PK710_00150"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOT08140.1"
                     /translation="MALERTLSIIKPNAVEEGHVGAILHRFEQEGFRFVAMKMIQLSR
                     QQAEGFYAVHKQRPFFQDLVAFMTRSKVVVIALEREDAIRKYREVIGATDPAKAAEGT
                     VRKMYGKNLEENAVHGSDAKETAAFEIGWFFAENELA"
     gene            complement(5245..6117)
                     /gene="sucD"
                     /locus_tag="PK710_00155"
     CDS             complement(5245..6117)
                     /gene="sucD"
                     /locus_tag="PK710_00155"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOT08141.1"
                     /translation="MAILVNKDTKVVVQGITGGAGSQHAKQCLEYGTKIVAGVTPHRG
                     GEMWEGKVPVFNTVKEAIQKTGADTSLIFVPPPGAADAIMEAAEAGARLIVCITEGIP
                     TMDMIQVRRYLEAKPVRLIGPNCPGIITPGQCKIGIMPGHVHLPGHVGVVSRSGTLTY
                     EAVGQLTALGIGQSTCIGIGGDPVGGIDFIDVLSEFNADPDTHGVIMIGEIGGNAEER
                     AAAWIKANMKKPVAVFIAGSTAPPGKRMGHAGAIIAGGKGTAEDKIAAMKAAGVAVAP
                     TPGDMGVTMQAMLR"
     gene            complement(6127..7314)
                     /gene="sucC"
                     /locus_tag="PK710_00160"
     CDS             complement(6127..7314)
                     /gene="sucC"
                     /locus_tag="PK710_00160"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOT08142.1"
                     /translation="MKIHEYQAKQIFARHGVPIPKGYPALSVDEAEAAARRLVEETNS
                     QVLVVKAQIHAGGRGKGGGVKLAKNGVDDARKLAEQILGMQLVTHQTGPEGQKVRRLY
                     IEQGLDIERELYLGMVVDREKRRVAMMASTEGGVEIEKVAAETPEKILTVHLDPVVGL
                     TAFQARQLAFGLDLAKHGKETVSHFVKLAMKLASLFTQEDCSLCEINPLVVTKQGTVV
                     ALDAKINFDDNADFRHKTWNELRDVDEEDAVELDAKSAGLSYVSLDGNIGCLVNGAGL
                     AMSTMDIIKHFGGEPANFLDVGGSATEEAVTKAFRIILSSPKVKAIFVNIFGGIMKCD
                     LIAAGVIAASKQVGLKVPLVVRLEGTNVELGRQMLADSGLAIQSATSMKDGAQKVVAA
                     ANK"
     gene            complement(7429..7890)
                     /locus_tag="PK710_00165"
     CDS             complement(7429..7890)
                     /locus_tag="PK710_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOT08143.1"
                     /translation="MPALYSLVALTFCLDGQRRVLVVQESRPDCWGRYYIPAGRGATG
                     EDPLRLACRVTLEKTGIHVEPLGVVGFEHNPPIGQYPGQLRVLVVAQAIGGTLKRVED
                     SHSLGAAWIAHDDVRGLKLRSDDFLPWLDDLMLGALPVLPATSWRALGGRP"
     gene            8043..10007
                     /locus_tag="PK710_00170"
     CDS             8043..10007
                     /locus_tag="PK710_00170"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003218.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HOT08144.1"
                     /translation="MNRPRNPDPSHGQRSLLLDLFEQPLSETSTKSLDDETEQTHSPD
                     DANDAPDRSSRTKNEPTRRATAEMMASRQREISVSEFFTKNRHLLGFDNPAKSLLTAI
                     KEAVDNSLDACEEAGILPDLVVEVDEVGEGRYRVAVQDNGPGIVRSQVPKIFAKLLYG
                     SKFHRLKQSRGQQGIGISAAGLYAQLTTGKPVVILSKTGNGKMARRIELRIDTRHNKP
                     EVLSDTEHDWQAVEHGTRVELELAGTYRGGRTSIDAYLEQTVIANPHMSLSYKLPKMA
                     EPLVFERAAHELPREPLEIKPHPHGVELGLLIRMMLEARGQTVKKTLVESFSRVSASI
                     AQQICDQAGVPASAKASQLDAEQIEAIHHALGTVRVLAPPASCVVPIGEELIIAGLRR
                     RFDAEFYTAVTRPPSVYRGNPFVVECGIAYGGRLERENPAEIMRFANRVPLQYQPKAC
                     AMTEAVYQTNWKAYQLAQPKGSLPIGPVAVVLHLASVWVPFTSEAKEAVAHYPELLKD
                     MRLAIQEAGRRLATHLRARHRTENALKRKSLFERYIPELGASLAALTGRQQEAIVQSL
                     LETLTSFVNIEVPESEGEPDPTPQGPSMPPPADALDGEAPISGRLLPIEDDRKGRQVF
                     MNHDEPTRNRPPHGDQRSPHVDDTLASRSE"
     gene            10016..11128
                     /locus_tag="PK710_00175"
     CDS             10016..11128
                     /locus_tag="PK710_00175"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003333.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOT08145.1"
                     /translation="MATTHTREQRAQQTIRKIEKLAAETLAQIQASDNPTLQIPIRAL
                     SNVSWNEDRRLIELGDRRQSREFFNLNMAKKFMQTFLVAAACKELLKSGKTTSIRDLF
                     YMTKHTIKGSRENTFDDQTESDPIIEDLEVTVDALREELHLFAAKRGSMVGPMTIVDA
                     GDTINLARMGSGGWSIPSITENHVIQFKQCEAEYILLVEKEAVWHRLNEDKFWKKHKC
                     ILVTGNGQATRGVRRLLQRMHNELKIPLYVLVDNDPWGLYIFSVVKQGSISLAYESMR
                     MAVPTARFLGLRTRDFKTFGLSDDVRIALEKEDENRARQMLSYPWFAAKHWQTEIKDM
                     LKAGFKMEIEALSNKDISFVTDVYIPQKITKRDWLV"
     gene            11514..12809
                     /gene="purB"
                     /locus_tag="PK710_00180"
     CDS             11514..12809
                     /gene="purB"
                     /locus_tag="PK710_00180"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOT08146.1"
                     /translation="MIPRYTPSEFAILWSDETRFGIWLDIELAACEAMESEGLVPIGT
                     AKTIRNLNLVLDPQRIHEIEKTTRHDVIAFLTHVEQLAGAPARYLHRGMTSSDVLDTG
                     FAILLTRATDALLLRLESLLEVLKRRSKEHSHTVLMGRSHGIHAEPVTFGVVLAGHYC
                     ELKRGLARLMVSREEIRTGKLAGAVGTYAHLSPAIERKALESLGLKAETVPTQVVARD
                     RHAALFGAMALIAAGIERLATNVRHWQRTEVSEAEELFSTGQKGSSAMPHKRNPILSE
                     NLCGLSRIVRAAVTPALENIVLWHERDISHSSVERMIAPDVTTTLAFMLDRATQLIEG
                     LVVYPEQMNRNVQRSAGLCFSESVLLALVDRGIARQQAYVMVQRNAMKAIEGHGSLLE
                     NLLSDEQVVERIGREPLIRCFDLEHSLRHVDEILARAFE"
     gene            complement(12739..13521)
                     /locus_tag="PK710_00185"
     CDS             complement(12739..13521)
                     /locus_tag="PK710_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT08147.1"
                     /translation="MLDGACIAVVVPAHDEEHFIHRVLDTIPAYVDFIFVVDDASSDH
                     TFRVAQQHGDARVHILRHAFNRGVGASIATGYRLALQANAHVVAVMAGDGQMDPDDLL
                     AVLRPVVDGKADYVKGTRLSHEEAYRMPALRRLGTRVFGWATSQVVGYPLTDSQCGYT
                     AISSNAIRKLDLHGLWPSFGYPNDLLFQLRMYDLRVAEVSVRPVYDKERSELKPRHAF
                     VIACLIARGAWRIRLRSPSIIRRHEPISHRHGEASAPNRNSE"
     gene            complement(13538..13987)
                     /locus_tag="PK710_00190"
     CDS             complement(13538..13987)
                     /locus_tag="PK710_00190"
                     /inference="COORDINATES: protein motif:HMM:NF021119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HOT08148.1"
                     /translation="MSGKLDQVVDHLLDGMHQISKIETVVGEPLQAGDAMLVPIHRLR
                     VGFGVAAIGAGGHGEATEGNYGGRGVGGGVQVEPIAVVAIAKDGKPRLMAIDGEPEQM
                     VGQLFEQIPDLLGRVLALVTRKREEHHSARFESGDTPKAELPEKEEE"
     gene            complement(14003..14668)
                     /locus_tag="PK710_00195"
     CDS             complement(14003..14668)
                     /locus_tag="PK710_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08149.1"
                     /translation="MLWVVLAVAVVCFITLAVVIVAPVRLEVSIKGQGEFGKFWAVAM
                     GGQLLFFTVSFAMAYGIDSVFQLHLFGRRLFRSSPVKRKGPTSEPASPSLEVMLQKAR
                     RLRREVERWFDLDDLLVFAVDLRHRVRMERLNGQLSYATPDLAMTGILLGSLYSFAGL
                     FAPFGSFHIEPLWVDVAKASGNLELAFRFYPGRMVLDAIVFTFKKIKLRQRENTRSVP
                     APP"
     gene            complement(14696..15067)
                     /locus_tag="PK710_00200"
     CDS             complement(14696..15067)
                     /locus_tag="PK710_00200"
                     /inference="COORDINATES: protein motif:HMM:NF021119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HOT08150.1"
                     /translation="MELDDLVSGLMKQLYRVSNSESVVGKPMSFADSAVVPLCRISVG
                     FGTGSGDVSGEMRKKGGAVDVGGAGGGLSVEPRAFVVVGKGGHAQMLSVRRGARAILQ
                     RAIDVLPETVNQVLPAKDDDK"
     gene            complement(15191..16117)
                     /locus_tag="PK710_00205"
     CDS             complement(15191..16117)
                     /locus_tag="PK710_00205"
                     /inference="COORDINATES: protein motif:HMM:NF018597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HOT08151.1"
                     /translation="MQTRQTKTWPWVLVSASLVFVVAPWLAQASDPFLVVPAAQVVER
                     SPAYRYANASEQEALDELDRRKILYQRVEPHGAVRTPIRLTGRLHGVHIHSVLAEHER
                     PTTPFEICDARLALALDDFSALLARRGIVELVHYTMYRPSVASPAASSQAWFGEASKV
                     GPPESWYVQAKKSPHQSSKKKKPPRPAASAQAGSRTKKPPHLASSTPRLNVSPPRHPL
                     GLAIDAGIFIKQDGTMLSVASHFRGQIGAQTCGPGVAPPQEPKARELWDIVCEAYDTK
                     IFTYVLTPNFDQAHQDHFHMDLTPDVRWFLYH"
     gene            16352..16804
                     /locus_tag="PK710_00210"
     CDS             16352..16804
                     /locus_tag="PK710_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOT08152.1"
                     /translation="MTSDRFKEMVEFAILREQEAVDFYTAMADNVSETHIKNVLRDFA
                     SQERGHKAKLQSILDGKNPTLVHKDVADLKIADYVVDVEPTQDLSFQGALIIAMKKEK
                     AAYRLYSDMATACDDPVVRDLFLWLANEEASHKLKFEILYDDLIFREN"
     gene            complement(16809..17252)
                     /locus_tag="PK710_00215"
     CDS             complement(16809..17252)
                     /locus_tag="PK710_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08153.1"
                     /translation="MNVSTKELQEQLVVDLKNWQKAEGAAANQMEKLSKATNHELIKL
                     VADVIHADSLRHAKVQQMVVDSIEKGAHALSPDDLAKVWDIIEEHIKTEQHMVANVRK
                     ALDSLQGRKMLVQQYLLEYLLFDEQKHDHLLEKLEGIKKGMYPDG"
     gene            complement(17452..18447)
                     /locus_tag="PK710_00220"
     CDS             complement(17452..18447)
                     /locus_tag="PK710_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08154.1"
                     /translation="MTHSRSFLVSAAWRWGLAVAVIISIGCAKTPIAQSPDPSVQTDP
                     AAPPPAVPAPPTPKDATEIARSLIPTAVTASLEMERLLHHPLGKRLIRLPDVVSILES
                     TDLDPQNDISSLFLASTGLSRQDEAILVIRHSMTPERLNEAIEKAIERSDPPGQWLNK
                     STVRVARIHIRSEPVGLVLADPDLLIVLQSERIATAKVFDGPLMYPPSDSQDDLVVSM
                     QNPSTALRPYVRGIPATLSEAQVRVRLVDDGGAVLVANARSTTAEQAVTDAEELTRLV
                     ERLTTVKVSVVRIHLFRPVVFRAEQEMVEARVSLRPDEVRKLISMMDMLISKGNP"
     gene            complement(18444..19622)
                     /locus_tag="PK710_00225"
     CDS             complement(18444..19622)
                     /locus_tag="PK710_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOT08155.1"
                     /translation="MKLEVCLPARVVFARGAIDRLGELAAPWGTRAALVRGGSHLERF
                     GTLERVRSQLNAVGIDCVEFTFVGEPDTIAVDTATQLARKHACDMVVGLGGGAVLDAA
                     KAVSGLLSLGGEALDYLEVVGRGKPIAAACAPMIAIPTTAGTGTEVTRNAVLTHKSSR
                     FKASMRSVHLIARVALVDPQLTDSVPPRVTASTGLDALTQLIEPFVSTGANPWTDGIS
                     LQGVKLAARALPKAWENGSDQAARDDMALASLMGGVCLAHAGLGVVHGFAAPLGAMFP
                     IPHGAACAALLVPGIEANVRALRQLDRAHPTLARFAQLGEILTEKRFATPQEAVDATV
                     DFVRDLVHKLQVPTLSSFGVSASDTPAVVAAAKKASSMRYNPVVLSDQALEQCFREAL
                     "
     gene            complement(19626..19931)
                     /locus_tag="PK710_00230"
     CDS             complement(19626..19931)
                     /locus_tag="PK710_00230"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOT08156.1"
                     /translation="MYVVCVTIWVKPGFEEAFIEATRHNHVGTRKEPGNLRFDVLRKA
                     DEPTRFFLYEAYRTEEDFVAHQKTQHYTTWKQTVTEWMSQPRQGVRHHSLFPDDTEW"
     gene            20214..21125
                     /locus_tag="PK710_00235"
     CDS             20214..21125
                     /locus_tag="PK710_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08157.1"
                     /translation="MPNSPFPGRCPQCQGPLGQYADSCPFCGTNLTGLRSEAPQGPAL
                     QLDAPLHPVAAARPRPIRPRPATPKPGIVARMGVSVVAGAALLVLALLAASAFFFLRA
                     TSKPIPLEAPPVVSSTPSPPPLNIHGIVLADGPRFDPTDVLPMIRERIVDPGDRSEVH
                     LLGIVVRGASEGAVNLDSQGASVTYQYLVVHRDPRAEKALERKGERVEMTLQRDPPPI
                     TRVEENPSTKTVTEPLCVWHAAWQAAVASGLPRTEIMDAVYALDAQGKHGVWSFSLRN
                     QPESNRWIDGQTCAIKASQPAQVNPPP"
     gene            21193..22041
                     /locus_tag="PK710_00240"
     CDS             21193..22041
                     /locus_tag="PK710_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT08158.1"
                     /translation="MTRPRDFGFRIECFPTRTRTLPPATHTNAYALGGSEVLIVEPAP
                     EDKTEQKAMIDWVQAMRRVGRRPLAIVITHHHPDHIGAAAVMQKELGLPLWAHAATQA
                     LMPHLSIDKSLVDGDRIVLDDPLQTWEVLHTPGHASGHLCLFERDLGVLLLGDMVATK
                     STILIEPSDGDMREYLKQLERLAALEARVGLPAHGEPIEQPSRFLRATHAHRMMRENK
                     IIDVLRSTAAFVALDEMLALVYDDTSVVLWPLAKKSLLAHLEKLQAEGKVHGNNAGYY
                     GWVGET"
     gene            22099..22356
                     /locus_tag="PK710_00245"
     CDS             22099..22356
                     /locus_tag="PK710_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08159.1"
                     /translation="MSGHDMGLMSQCNEDHEAAGVAAATLYLLENSIDFLETHKQDPA
                     NLKPIEDLIRMLRDTDRDRRARALAAYLGKLSVQLESWSLR"
     gene            22353..23039
                     /locus_tag="PK710_00250"
     CDS             22353..23039
                     /locus_tag="PK710_00250"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HOT08160.1"
                     /translation="MTPGRGRIVRDGPSMTSLTLSSLPTLPACGMVAQGARGRIVRRE
                     RLEISEERSGILAVARQQAQELMQQAQRDAEEVRREAEQRGVADGAAKLAAAWLNLER
                     REATLDVESTERTIAIARLLAERLLGKSLELDPELIADMAKEAMVHLWRSHRVVIHAH
                     PHDVPMLTKHVTTFGMPPERIEIRADDSRQRGSLRFMSDLGELDGELAPQLDRLVEVI
                     RQELGNRIYP"
     gene            23036..24049
                     /locus_tag="PK710_00255"
     CDS             23036..24049
                     /locus_tag="PK710_00255"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOT08161.1"
                     /translation="MSTPHFPPPAPDVQRSVFEGASPEPVSGVPPEPPRRNRYAIHLG
                     LFVATVISVLFAGNQELLLGDEPLTLANLWKGWTFAVPLMVILLAHEFGHYIAARWHR
                     VHASLPYFIPMPISPFGTMGAIISMKGRIGSRKALLDIGASGPLAGMVFAVPILVWGL
                     LHSEVKEITGSGLLEGQCLLYSLLKRLTVGPIPEGSDVFLHPTAFAGWVGLLITMINL
                     VPVGQLDGGHVAYALIGPRQNAIARMVHYGLLAVFAVNFGYHFLRHFQTEPLGQTVGR
                     AAEMSMFWLVWFGFLFLLRALSGGNHPPTEPGELSRGRKAIAIGTLLLFVLLFMPTPL
                     SQY"
     gene            complement(24238..24732)
                     /locus_tag="PK710_00260"
     CDS             complement(24238..24732)
                     /locus_tag="PK710_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08162.1"
                     /translation="MNESRSGAHVERSGSVGPFSQRDSPEAQTGSTHAPLEASQTCKP
                     AQGSSAHLFPSAAHTARAAVPGLPSQRAEPGSHPVSTLSQDPVVTLHVVPVGHICENQ
                     PRPSARHISTLPSCCEEHRWLAGVQASPGIAGGTSIGFGSEDVHPKKGMHKLSPRQAD
                     KQAR"
     gene            24791..26398
                     /locus_tag="PK710_00265"
     CDS             24791..26398
                     /locus_tag="PK710_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08163.1"
                     /translation="MARLCSPGDRQCSNDYLQVLVCDTSGTSLEIIETCADDEACSKG
                     ICEKSTMPLGQTVRVTSSDGPFSVSPGRYAVVVVNTDTSNDEFVDYPLHLDGSISDPV
                     TTTFRHLEPQKVSDPAAVSRGYPMSKGRTATVFPRPGPEPNDAETRTFFVPRYETGGE
                     ETIPRTATLRASGQHVRVWEDQSTSSAGTLVPDTVLDELMDRLDHAVLPRAKAMVGEP
                     TDVDGNGKIDILFTDVLPDAVKAFCHPSATLFAPGTFSVSYDYGEVVYAHGIDESAPV
                     WETSTLMSHEVAQLIVLSQRLEPFLSDPESIPPWVEADIYAVEGLASLVMGWSGQSHA
                     WPVTTALEHPQEASLARLTASSYLADATANLASYGFGTLVQEYLFDQAGAMVVSDGGA
                     TIQDGGGKAYLQAFTAGQEGWDRIHPLDGRPVSTWYVDFATALLLVHLDGKLAPSTAK
                     DPRTAFAPTVLDTKYGGFLGPTLRWEHVRDSSKKGPIHRLMPWSQHPASMRRGGLSFL
                     AVSVGDSGATMQVSEDATTAVWVRHRP"
     gene            complement(26409..27503)
                     /gene="asd"
                     /locus_tag="PK710_00270"
     CDS             complement(26409..27503)
                     /gene="asd"
                     /locus_tag="PK710_00270"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOT08164.1"
                     /translation="MIELPKKLRVAVLGATGMVGQRFLALLEHHPWFDVTLVAASGRS
                     AGLSYAEAVHDRWALPSPLPPSFASLPVADASSVSQIADEVDFVFCAVDMPKDAIARL
                     EEAYAKAETPVISNTSAHRTTPDVPMIIPEVNPEHANVIPDQRKRLGTTRGFIAVKPN
                     CSVQSYVPAIHPLMDFRPRHVVVSTYQAISGAGKTFESWPEMQDNVIPFIAGEEEKSE
                     QEPRKIWGRLAHGVIVPSAQPVISAQCIRIPVTDGHLATVFVAFEKRPCREEILARWR
                     NGTSLPQRLGLPTAPCPWLTYFEQDARPQTRLDRDLGKGMGISVGRLRTDSIFDIRFV
                     ALSHNTVRGAAGGAVLTAEWLTHEGYITAK"
     gene            complement(27573..28127)
                     /locus_tag="PK710_00275"
     CDS             complement(27573..28127)
                     /locus_tag="PK710_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08165.1"
                     /translation="MTRTSRLLQVATISMVMMGAGSAHADVSSWLFVGGGAHWLSEQK
                     SGYEIAPAMQLDLGLGTPPSGPVVLGVLTRSLTHFGRGTDLALALRATTREFSSGGFG
                     LALDAGAYKRWWGMESAGPMASLQLGAPYGLQLSLNGALGSDDHKTFGITFGIDLLRL
                     TVHRLAGEQWWKNPRPAWRPDTAP"
     gene            complement(28188..29177)
                     /locus_tag="PK710_00280"
     CDS             complement(28188..29177)
                     /locus_tag="PK710_00280"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT08166.1"
                     /translation="MDTPRLEGKALITGASGFIGGRLRDTLIDQGVDVIAVRRKGSPP
                     AKRGRSIELSYDDRDGIARMIRHEKPDWVFHVAGVTKGVTYDDFRNGNVMPTRNLLET
                     VRDEHPAVQRFVHVSSLTSYGPSAPDQPHVESSPRRPIEYYGESKLEAERMVEAEHGV
                     PWTILRPGGVYGPGDVDYFELFKQIERGFDVYFGNKNRVFSGIYVDDFVSALLFSAQH
                     PDTVGKGYFVCDGVPVTWQRFQQQIVKASGKRVRTLDLPEIIVDIAALGGEWMTRLDH
                     KPRLFNRQKAKMGAQPAWTCKSDALRALGWTSNTTIEQGIPATLAWYREHHWL"
     gene            29207..30676
                     /locus_tag="PK710_00285"
     CDS             29207..30676
                     /locus_tag="PK710_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOT08167.1"
                     /translation="MNTSKNGDSTVAGVSSAVDARRVEPQGADIFGTGETAVSYHDNI
                     LRAIGRTPLVRLSRMVGPRDATVLVKPEYMNPGGSIKDRMALYILEKAEREGKLRPGG
                     VIVENTSGNTGVGLAMAAALKGYRCVFTIPDKMSAEKVNTLKAFGAEVHICPTNVPAD
                     HPDSYYETAKRLVGELGAFFPNQYHNPVNIEAHYHTTGPEILDQTEGNIDYFVAGLGT
                     GGTMSGAGKFLKDKVPTLKNVGVDPEGSVYHSLFHTGKLSTPHVYKVEGIGEDMACAA
                     LDLSVLDDVRQVNDRQCFIAARRLAREEGIFGGGSAGAAVHVALQIAKEAGPGKVVVA
                     ILPDGGRAYISKFYSDEWMRDMGFLEPDPSVARVEHLLQDKPPVVVATVGESTRQVVL
                     RMQKHGFSQLPIVNGQGRAVGMVHESDVLGSLLSGDRNLDQPIEPAALQGVVSLRTPI
                     HEVEKVLADDNVAVVIEDGRVIGIVTKIDLITHLANPPR"
     gene            30688..31827
                     /locus_tag="PK710_00290"
     CDS             30688..31827
                     /locus_tag="PK710_00290"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF005871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HOT08168.1"
                     /translation="MRFETNAIHAGQSPDPTTGAIMTPVFQTSTFVQEAPGKHKGYEY
                     SRTQNPTRAALEACVAALEGGRHGLAFSSGCATTDAVMHLLSSGDHVVSMDDVYGGTR
                     RLFEKVWARHGIDFTFADLSRPGELEAALTDRTRLVWVESPTNPLLKLVDLEATARSC
                     KQRGIPLVVDNTFMTPYFQKPLALGADIVVHSATKYLNGHSDVVGGLLVTNDEALRDR
                     LAFIQNSAGAILGPMDSFLVLRGLKTLHVRMDRHQSNALELATWLETHPRVKIVIYPG
                     LTSHPQHELARKQMSGFGGMITLRLDANLEQAARVISSCRVFSLAESLGGVESLIEHP
                     AIMTHASVAPEERAKLGIDDGLIRLSVGIEAVEDLREDLERAFRD"
     gene            31941..32996
                     /locus_tag="PK710_00295"
     CDS             31941..32996
                     /locus_tag="PK710_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08169.1"
                     /translation="MSFSLLVSQHHPKSPDRVEAWETAFLELQVVLAYIDSVLAPAER
                     MTLSLLNLAKWDEKDNRRQVDELTRSIEKHGIDQAVRRVIRKLEELFEQEDPTIRPHL
                     AQEILQSSVAIVVADDVISDNEESFLRDLLAPALGMDEAHVMEVVKAASGRLSRFRAY
                     AERGLECYLMLAELQEGPPQLNSLAGDKLPGFLGAVDQWVVQHRLGSSRVMGYFLGAM
                     GGIFWIDSYEQHLSEMTQLVAAARTLSQRAGPEARLRAIHHELTQLRNRGVDANGHRA
                     IARHVTNALSRMASLNEAQRNRFRDAIAPALEIDHDALVESASQRASLIDLHDNLVGA
                     HSPEQDKTESQWWRFWK"
     gene            33056..33958
                     /locus_tag="PK710_00300"
     CDS             33056..33958
                     /locus_tag="PK710_00300"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT08170.1"
                     /translation="MSLGFLSEGELFSLGCALIWAVGVILFRVSGQRIEPIALNLFKN
                     LVGLVLFFITLPLVGVSFYPEGTTPTDLLTLAISGIVGIGIADSLFFASLNRLGAGNT
                     AIVDSLYSPFVILCAFVYLQESISSAVLVAMLIMTAAILVGTWDPSLPSEPQSRKQIT
                     VGVALGVLGMFLMAVGLVIAKPVLEHAQAWWVTTMRLVAGTAFIAIQGLLPRHRASVL
                     AILRPSRHWRVAVPAGIIGCYLAMITWIAGLKLTLASIASILNQTSTLFVVLLAAIFL
                     KEKVTLRKGAAVAMAFVGAVLVTW"
     gene            complement(34043..36220)
                     /locus_tag="PK710_00305"
     CDS             complement(34043..36220)
                     /locus_tag="PK710_00305"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOT08171.1"
                     /translation="MKRIFGITCLACGCLGLGCHGGVHGNAQTSSAIRSTTASMVASS
                     TPPDESTPPLSFSSDEGMWLLNHFPSEVLERRHGFAPTQAWLDEVMRSSVRLARGCSG
                     SFVSSRGLVMTNHHCAVGCIQQLSTTRKDYIKSGFTAKQATDEPRCPEMEINQLIEIT
                     DVTDRVNEATKGLDETKANDARKQQMSAIEKSCATSDALRCDVVTLFQGGRYHLYKYR
                     RYQDVRLVFAPEVSIAFFGGDPDNFNFPRYDLDVTFLRVYENGKPASTPSHFRWSPHG
                     AEQGQLTFVSGHPGRTSRMSTVSQLAYMRDHGVPAVLMRWLEIRGAVREFQKRGVEQA
                     RVSGTTLFGAENAIKALRGRQQALLDPMFFSQLQQKENQLRDRVKADADLEAKYGDAW
                     DRIAAVQAAKQRLANEYALVEAGQGFWSTLMDHARKIVRASDELPKPNEQRLREYADS
                     NLPALQQELFSTAPIHQEFEEMKLALSLTRLRELLGTDHPVVKAALGNESPEQVAKKT
                     IAGTKLTHVDERKRLYESGKTAVQASSDAAIVLARTIDPWARQIRERFEQQVEAVETR
                     NAERIAAARFAVFGTSVYPDATFSLRLAFGVVQGWTENGQQVGPFTTMAGAFERHTGA
                     PPFDLPASWLTAKSKINGSTPMNLVSTHDIIGGNSGSPMFDTQRQIVGLIFDGNIHSL
                     GGDYAYDPRSNRAVSVHSSAILEALDKVYDARSLREELLENNQ"
     gene            36427..37215
                     /locus_tag="PK710_00310"
     CDS             36427..37215
                     /locus_tag="PK710_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08172.1"
                     /translation="MKHSLFSLVIALCPLAAGCAMGVQDEGFEAVDSTEYAATACGAN
                     YGEAFEYYKRAVDDSKDRLRYGVCQSENGFMWAIADNASRAVMTCGAFRDVIRTSPWA
                     APLRQALAPSLTLRSLTGELLVIKDSPWQNWSHVEEFFEQGVSFWARSQGAYGYSVKV
                     DFGANGSAVWGEQTYDESTGEISWRVVSATYAITKTSSQASGRRLVTVTHDGIVEKFS
                     LGVIDAEAWKDAPLFVLDPLGTGPEPGQGATVPQLFSLVTECDA"
     gene            complement(37359..>37559)
                     /locus_tag="PK710_00315"
     CDS             complement(37359..>37559)
                     /locus_tag="PK710_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL17 family ribosomal protein"
                     /protein_id="HOT08173.1"
                     /translation="QELAPQFATRAGGYTRIIKLGPRRGDNAPMSLIEFVTEGETTAT
                     SEPAAEPAVAETGPNSTPEATT"
BASE COUNT         8200 a        10813 c        10593 g         7953 t
ORIGIN      
        1 gatccccaat catgggattg aggttgggca acgccaacgc cacccccacc ggctcgccgt
       61 caagttctac aattcgcgtc agttccggga tcaagatcaa cttcatatcc gaagccatct
      121 tcgcgagttc tggttccgtg agccgcacaa atccccaatt tgaactccac gcatcgttgt
      181 acacatccat cacgatgcgg atgtctcttt cgacatgctt catgtccgcc atacgcgaag
      241 tgatttcggg catttgcgca atggctgcat gggctttcaa agcccgttca ggcatctgcc
      301 cgatggtata atgccaagca aatagatctt tgaccttcga taatcccgcc gcttcgatga
      361 gctttccttg gtaaggacga tgatgcggca tcatgaaaaa cggcggcgtt tcgaagccct
      421 ccaccagaca tccaagctcc tcgttgacac ataaggacat cgggccacgc atcttcttca
      481 tctgacgagc gcgaagccaa cgacccgcgt gatcgagcag agccttggct acctgctcat
      541 catccaccgt atcgaaaaat ccgaagaacc cacaatcatc gttgtaacgt gccagatgct
      601 ccctatcgat ctgggccgta cacctgccca cacacttgcc cctgcgaaac gcggtcaaga
      661 caaccccttc tccatgattg aaaaaagggt tccgcaagct caaccgatcc cgcacgtcca
      721 tgtccaaagg tcgaacataa caaggatcgt cacgatatat ggtgtccacc acattgagga
      781 agggtcgcaa attgccaccc aagcgtgtct cacgaacctc gactgtcatg actcgatgcg
      841 actacctcgc gtcgcaccca gacgcaatgg ggatgatgaa gatcgacgac gagcctgttc
      901 tcccactggg ccaagctcag cctaatgccc cgcctgcgca atcacccgag ccaaaatgac
      961 ctgcgccagc ccgaaataaa tcagaattcc aaaaacgtcc acgaggctcg caatgaaggg
     1021 cgtggaactc gtcgccggat caagccccac tcgcttgatc atcaacggaa gcattccccc
     1081 aatcgtgcag cccatgatcg aaatggcaac gagagccgct ccgatgagca ctgcaaaatt
     1141 ccacccatcc ccccacaaca aaacacgaac agctcccacc agggcaagaa tcaaccccaa
     1201 cacaaccccc tgcaccatct cccgaaccaa cacccgatac caatccccca tctgtatctc
     1261 cccaaccgcc aacccccgaa tgatcaacgt cgaggattgc cctcccgaat tgccccccgt
     1321 cgaaatcaat agcggaacat aataactaag ctgagccacg gactcgatca catgatcgaa
     1381 atgccgaagg gccgtgcccg taaacatctc accaaggaac aagaccataa gccaaacacc
     1441 acgcttcttg atgaacgtcc acgtcgtggt atcaaaatac ggattttcga tcggctgaac
     1501 accaccaagc ttgtgaacgt cttccgtttg ctcctcggtc atgacgtcaa cgatgtcgtc
     1561 gatggtaatg atcccatgga actccccacg tccatccacg acaggaaccg cctgcatatc
     1621 gtacttcgcc atgagcttgg ccacttgctc ttggtcggtt tccggcgtca cggaaatcac
     1681 gttttcaacc atgatttcat cgatgcgttc cgtcggatcc gcaagaagga tatcccgcaa
     1741 cgatgcgatt cctatgagtc gatcttgcgg gtcgacgaca tacacgtagt ggatgagttc
     1801 aacgtcttca ccctggcgtc gtacctcctt gatcgcatct tccgcggtcg catcacgatg
     1861 cgtctcgata taatccgtgg tcatgagatg acccgcgctg gtttccggcc agcgcgtcag
     1921 ctcttccacc tcttcggccg cttcagggtc gtacttctcg agagtctcga ggagtttttc
     1981 ccccacgttg tcagggagcg cggaaaacaa gtccgcccga tcgtcggcgg acatctccgt
     2041 ggcaatgcgc gccgccttca acaacccgat tttgttgacg agagcttcct gacgtccttc
     2101 aggcaaacgc tcgaaaatat ccgcggccgc atctggagcg agacgctgaa gcaaagcacc
     2161 cgcttcgtca tcgtcaagct cgaggacaac gtctgccaag tcctcgggat ggatctcatc
     2221 gagcaactca cgcacgtcct ccggattgtc acggagaagg tcgtgaatct caggaccgat
     2281 gaggtgagcc aatcgcatgt cccaatgccc ttgcgcacaa aacgccggga aagaaaaacg
     2341 aaaacgcttt caaggaggcg attccgaaac ccattcccat ccacggccgc gccgttttcg
     2401 catcggcacg cacgacagac cggaaagact cggttcggac tatccgacga actcgtaacc
     2461 tattgatatt acattataat tatacgattc cccaccaaga cgcgtctatt ttccgttgtc
     2521 gctcggatcg agtcgatcag cctcggtcaa catctcctca acgagatagg cggaaacctc
     2581 cggaagtcga tccatgagtt gcggggcgat ggacatcata tcttccacac ccagttcatg
     2641 cacctccgag gtcatgtcca ccacaatgct atccgaagtc gactccgacg gcgggggcgc
     2701 ggacgagggt gtcgaaagtt gcccatgggc cgtcatgagc gcaaaggact gacgaacagc
     2761 aagctgcatg gctttggcgt cgttccaacg atcatccatg gagaacttca acgctcgatc
     2821 gatgacaccc accacgacct tgggaaggtg cggtgccact tcacccacgg aaatcgcggg
     2881 tttagtcatg gccgccaaca atcgatccgt cggtgtttcc gcttcatgca ccacccggcc
     2941 cgccaaggac cgaaacatca ccgctcccac cgcaaacaag tcggtccgac catcgacacg
     3001 atcccattgg ctacgagctt gttccggagc catgtaggaa ctcgttccca tcacgactcc
     3061 cacacccgtg agctgcgttt tcggatcggc ctctttgaga cgggccagac cgaagtccaa
     3121 cactttgacc tgtccttgtt tcgtcagaaa aatattgcct ggcttgatgt cccgatggat
     3181 gacaccagcg ccatgcgccg tagccagaac gtccaaggtt tggtcggcaa tggcaagcac
     3241 gtgttgcact gacaagacgt tgccactgcg ttgcaacagc gcttccatcg attggccttc
     3301 caatagctcc atcaccaaaa atacggctcc atcctcggcc gtatcatcat ccaatacggt
     3361 aaccgccccg ggatggttga cgcgattcgc caagtatccc tcgcgcaaaa accgttcgcg
     3421 aacactcgat gcctttggaa agatgggatg taatactttg accgccacgc gattgccgtt
     3481 tcgatgcgtg gcctcgtaca ccgccgccat tcccccaatg tcgagaagct tatccagatg
     3541 ccactttcca caaagcatct gccccacacg cttttctgca accatacgaa gctttggatc
     3601 gttacccatc catatcgacc ttgtcgccta acaagccctg ccgtcaaggg ttacgtcgaa
     3661 ttcgtgggag cctacgaaag aaaacggttc gacacggcca tacccaggtc atccgccgag
     3721 cgctgcggta aaattcgaag agtcactgtc gttggctcca tcgataacgt cacccccgtg
     3781 agcgcagaag ccacggacaa gacgtcacga ctcggcatca ccacacgagg ccaaacgcac
     3841 ggcaggttcg ccgcgggatg atcgtcatcc ctgcggcaca agacagacgc tcccccttgc
     3901 tcatccaact ccgccgacac gtgcatttgc tttgcccctt gatgcgctac cgagccaaac
     3961 gcaatactca tcatcggcat caacacgcgt gcatcggtcc acaccgtggc tccttccgcg
     4021 gcacgagata gcgccaccgg cacaacctca ggttggtttg gatgcggcga gacacgatgg
     4081 gtcaacgtct cccatagcaa ggcattcaag tccaactccg tgggcgcgct atatgtcgca
     4141 ctgtccagca aatcgatgag ttctcgaacc acatccaagt ccggaagaac ctccgtaatc
     4201 tgcctttcga gccgaagcct cgatttggct cccatgtctt ttttggcggc attcgatacc
     4261 gcgtggcaaa gctgatcgac acgatcacgc gcaaaatcgc tcaagctacc caccggcagc
     4321 tccgcttttc ggcttcccac catatcgacg atcgacacca aagcatctct gaatggcgag
     4381 catccggcgt ggatctgggc cacggctttt tcgagcgctc gcggcccaat ctttggagat
     4441 ttgagcaagt tctcaaggtt gcgcaaactc gcaagaccat cacgcacata gccttgcgcg
     4501 gtatcgaaac catcttcacg agtcgttgtt cgtgtttggt attgcatccc ccaccgacct
     4561 gtcggctcca aggtgccatg acccccgcgg gtaatgccta tgctaactcg ttttccgcaa
     4621 agaaccaccc aatctcaaac gctgccgttt ccttcgcatc agaaccatgt acggcattct
     4681 cctccaagtt ctttccgtac atcttgcgca ccgtcccttc cgccgctttg gccggatcgg
     4741 tagccccaat tacctcccga tacttgcgga tcgcatcctc acgctcgagc gcaatcacca
     4801 cgaccttgga ccgcgtcatg aaagcaacca ggtcctgaaa gaacggacgc tgtttgtgga
     4861 cggcataaaa cccttccgct tgttgacgac tgagttggat cattttcatc gcgacgaagc
     4921 gaaagccttc ttgttcgaat cgatggagga tggcccctac atggccttcc tcgacggcgt
     4981 tgggtttgat gatggatagg gtgcgttcga gcgccacggt cacctcccat gaaagaattg
     5041 gaatctcgct agcttacaag caagatccta gggttgcgac cgctgtgtag tcgcttggac
     5101 gggcagccgc tacaggaatt ttatcggggg gggatggctc gaggcatacg cacgagccag
     5161 cggcacgggc tgttccgacc accggagata ttctttagga ttttcacaga tttgctgagg
     5221 tgaggaggcc ccatcgacga cggtttatcg cagcatggcc tgcatggtta cgcccatgtc
     5281 tccgggtgta ggggcgacag caacgcctgc cgctttcatg gcagcgattt tatcctccgc
     5341 ggtccctttt ccaccggcaa tgatcgcgcc cgcatgcccc attcgcttgc ctggcggcgc
     5401 ggtggaacca gcgatgaaca cggccaccgg ctttttcatg ttggctttga tccaagcagc
     5461 ggcacgctcc tccgcattgc ccccgatctc accaatcatg atcacgccgt gggtatccgg
     5521 gtcagcgttg aactccgata aaacatcaat aaaatcaata cctcccacag gatcgcctcc
     5581 gattccgatg caggtggatt gaccgatgcc caaggccgtg agttgaccca ccgcctcata
     5641 ggtcagcgtt ccacttcgag acacgacacc gacgtgtcca ggcaaatgaa catgaccggg
     5701 cataatccca attttgcact gcccaggggt aatgatgccc ggacagttgg ggccgatgag
     5761 acgcacgggc tttgcctcga gataacggcg cacctgaatc atatccatgg tgggaatgcc
     5821 ttccgtgatg catacgatga gacgtgcgcc agcttcggcc gcttccatga tcgcatcggc
     5881 agcgccggga ggaggaacga agatcaacga tgtgtccgcg ccggtttttt gaatcgcctc
     5941 tttgacggtg ttgaacaccg gcactttgcc ttcccacatc tcgccgccac gatggggcgt
     6001 cactcccgca acgattttcg tgccgtattc taggcactgc ttggcatgct gggagccagc
     6061 tccgccggtg atgccttgga cgacgacctt ggtgtctttg ttgactaaaa ttgccatctt
     6121 ggacctctac ttattcgccg ccgcaacgac tttctgcgcg ccgtctttca tggatgtcgc
     6181 actttgaatc gccaacccgc tatccgccaa catttgacga cccaattcca cgttggtgcc
     6241 ttcgagacgg acgaccaaag gaacttttag acccacctgt ttggaggcgg caatcacgcc
     6301 cgcggcgatg agatcgcact tcatgattcc gccgaagatg ttgacgaaaa tcgctttcac
     6361 tttggggctg ctcaagatga tgcgaaacgc tttcgtgaca gcttcttccg ttgcgctccc
     6421 ccccacgtcg aggaagttcg cgggctcacc accaaaatgc ttgatgatgt ccatcgtcga
     6481 catcgcaaga ccagcgccgt tgaccaggca acctatgttg ccatcgagcg atacatacga
     6541 aagccccgcg ctcttggcat caagctcgac cgcatcctct tcgtcgacat cacgcaactc
     6601 gttccacgtc ttatgacgaa agtccgcgtt gtcatcgaag ttgatcttgg catcgagagc
     6661 gaccaccgtc ccttgtttgg tgacgaccag cggattgatc tcgcaaagcg aacaatcctc
     6721 ttgcgtgaaa agagatgcca acttcatggc aagtttgaca aagtgcgaaa ccgtttcttt
     6781 tccgtgcttg gcaagatcga gcccaaacgc gagctgacgc gcctgaaatg cggtgagacc
     6841 gaccaccgga tcgaggtgga ccgtaaggat tttttctggc gtttccgccg ccactttctc
     6901 gatttccacg ccaccttcgg tggacgccat catcgcaact cgacgcttct ctcgatccac
     6961 aaccatgcca aggtatagtt cgcgttcgat atcgagccct tgctcgatgt aaaggcgtcg
     7021 aaccttttgc ccttccggtc ccgtttgatg cgtgacaagt tgcataccga gaatttgttc
     7081 ggcgagcttt cgcgcatcat ccactccgtt tttcgcgagc ttgacacctc cgccttttcc
     7141 tcggcccccg gcatgaattt gagccttgac cacgagtact tggctgttgg tttcctcaac
     7201 gagacggcgt gccgcggctt ccgcctcatc gacagaaagc gcggggtaac ctttggggat
     7261 tggcactccg tgacgagcga agatctgttt ggcttgatat tcgtgtatct tcatggcgaa
     7321 ttcccttgca gagagccggg aacctaccat gtaacgcgcg tattcgggaa ccagggctac
     7381 gagaaaacaa cgtatccatc acgaaagagg agcaatggga ccaggctcct acggtcgtcc
     7441 ccccagcgct ctccacgagg tcgccggcaa gaccggcaaa gcacccaaca tcaggtcatc
     7501 gagccaaggc aaaaagtcat ccgaacgcaa tttgagaccc ctcacatcgt cgtgggctat
     7561 ccatgcagca cccagagaat ggctgtcttc gacgcgtttc agggttccac cgatggcctg
     7621 agcgaccaca agtacgcgca attgccctgg atattgccca atgggtggat tgtgttcgaa
     7681 gcccacgact cccagaggct ccacgtgaat accggttttt tcgagggtca cccgacacgc
     7741 gagccgcaaa ggatcctccc ccgtcgcccc acgacccgcg ggaatgtagt accgtcccca
     7801 gcaatccggc ctggattcct gcacgacaag tacacgccgt tgcccatcga gacaaaacgt
     7861 caacgcgacc aatgaatata aagctggcac aaactctatc catactcgac tcgaagcaag
     7921 attccagtct tgcgccgcac gctacccgtg gcatggtgtt ggtttcccat gtccggtgaa
     7981 ccaggcccac ccgaccgttt gcttgacatt tgtacaggct accacctacg tcaaacgctg
     8041 tgatgaatcg accccggaac cccgatccga gccacggtca gcgctctttg ttgctggacc
     8101 tattcgaaca accgctttcg gaaaccagca caaaatcctt ggatgacgag actgaacaga
     8161 ctcactcccc cgacgacgcc aacgatgcgc cggataggag cagccgcacg aaaaatgagc
     8221 ccacccgtcg cgccaccgcg gaaatgatgg cctctcgaca acgggaaatc agcgtcagcg
     8281 aatttttcac taaaaacaga cacttactgg gattcgacaa tcccgccaag tccctgctga
     8341 ccgctatcaa ggaagccgtc gacaactccc tcgacgcctg tgaagaggca ggcatcctgc
     8401 cggacttggt cgtggaagtc gacgaggtcg gggaaggccg ataccgcgtg gcagtacaag
     8461 acaacggacc cggaatcgtc cgaagccaag tgccgaaaat tttcgccaaa ctgctgtacg
     8521 gttcgaagtt ccatcggttg aagcaaagcc gtggacagca gggcatcggt atcagcgccg
     8581 cgggcctata cgcgcaactc accaccggaa aacccgtcgt cattctttcg aaaacgggaa
     8641 atggaaagat ggctcgacgt atcgagcttc gaatcgatac ccgtcacaac aagccggagg
     8701 ttttgtcgga cacggaacac gattggcaag cggtagagca cggaacccgg gttgaattgg
     8761 aactggcggg aacctatcgg ggcggacgaa cgagcatcga cgcctacctc gagcagacgg
     8821 tgattgcgaa tccgcacatg tctctgtcgt acaagcttcc gaagatggcg gagccattgg
     8881 tattcgagcg agcagcacac gaactgcctc gggaaccgtt ggagatcaaa ccgcatcctc
     8941 acggtgtgga gcttggattg ctgattcgca tgatgctcga agcgcggggg cagacggtca
     9001 agaaaacgtt ggtggaaagc ttttcgcgtg tgtcggcgtc gattgcacag cagatctgcg
     9061 atcaggcggg ggttccggct tcagccaaag ccagccaatt ggacgccgaa caaatcgagg
     9121 ccatccacca cgctctcggg acggttcgcg tgcttgcccc tcccgcatct tgcgtcgtgc
     9181 ccatcggcga ggagttgatc atcgccgggc tccgtcgccg cttcgacgct gaattttata
     9241 cagcggtcac tcgtcctccc tcggtatacc ggggaaaccc gttcgtggtg gagtgtggca
     9301 tcgcctacgg aggtcgattg gagcgggaaa atccggcgga aatcatgcgt tttgccaacc
     9361 gggtgccgct gcaataccag ccgaaagctt gtgcgatgac cgaagcggtt taccagacga
     9421 attggaaagc gtatcagctt gcacagccga aagggtcgct gccgatcggt cctgtggcgg
     9481 tggttcttca tctcgccagc gtgtgggtgc cgttcacgag tgaagccaag gaagcggtgg
     9541 ctcactatcc cgaactgctc aaggatatgc gccttgcaat tcaagaagca gggcgccgac
     9601 tcgccaccca ccttcgtgca cggcatcgca cggaaaacgc gctcaaaaga aaaagcctat
     9661 ttgaacggta tattcctgag ctgggggcaa gcttggcagc gcttaccggc cgacaacagg
     9721 aagcgatcgt ccagtcattg ttggagaccc tcacgtcttt cgtcaacatc gaggtaccgg
     9781 aatcagaagg ggaacccgac cccacgcccc aaggcccaag catgccacct cccgccgatg
     9841 ccttggatgg tgaagctccg atcagcggaa ggcttttgcc tatcgaagac gatcgaaagg
     9901 ggcggcaagt cttcatgaat cacgacgaac cgacgcggaa tcgtccgccc cacggcgacc
     9961 aacgctcgcc ccacgttgat gacacgctcg caagccgttc ggaatgacaa gcaccatggc
    10021 cacgacacac actcgcgaac aaagagccca acagaccatc cgcaaaatcg aaaaactcgc
    10081 ggcagagacc ctcgcccaga ttcaagcgag cgataatccg acccttcaaa ttcccatccg
    10141 cgcgctgtcc aacgtgtcct ggaacgaaga ccgacgcttg atcgaactgg gggaccgtcg
    10201 ccaaagtcgc gaatttttca acctgaacat ggccaaaaag ttcatgcaga cttttttggt
    10261 ggccgcggcc tgcaaagaac tactgaaatc gggcaagacc acgagcattc gtgatctctt
    10321 ttatatgacc aaacacacga tcaaaggctc tcgggaaaac accttcgatg atcaaaccga
    10381 aagcgatccc atcatcgaag acctcgaagt cacggtcgat gccttgcgcg aagaattgca
    10441 cctctttgcc gccaaacgcg gctcgatggt cggccccatg accatcgtcg atgcgggaga
    10501 taccatcaat ctcgctcgca tgggttcagg gggatggagc ataccgagca tcacggaaaa
    10561 ccatgtcatc caattcaagc aatgcgaagc agagtatatt ttgctcgtag aaaaagaggc
    10621 cgtctggcac cgcctcaatg aagataagtt ctggaaaaaa cataaatgca tcttggtcac
    10681 cggaaacgga caggcgaccc gaggcgttcg tcgactgctc cagcgcatgc ataacgaatt
    10741 gaaaatcccg ctgtatgtgc tcgtcgataa cgatccctgg ggcctttata tcttcagcgt
    10801 ggtcaaacaa gggtcgatct ccctcgctta cgaatccatg cgcatggcgg ttcccaccgc
    10861 tcgatttctc gggcttcgca cccgggattt caagacgttc ggcctgagcg atgatgtgcg
    10921 tatcgccctg gaaaaagagg atgagaatcg ggcgcggcag atgctttcct acccgtggtt
    10981 cgcggccaag cactggcaga ctgaaatcaa ggacatgctc aaagccggat tcaaaatgga
    11041 aatcgaagcg ctgtccaaca aggacatcag cttcgtcacc gacgtttaca tcccgcagaa
    11101 aatcacgaaa cgcgactggc tggtgtaggg ctcgcggcgg gggtgacaaa aagagcaacg
    11161 cccggacggg ggggggcacg tgaccgggcg ttgagaagtt gaggcaacgt cagggacgat
    11221 aaggggggtg gtctcgaccg acgctgcgcc agtttgttat gcaatcccaa taccatctat
    11281 ttgatcaata ttattcggta gttacaacat tgaaggtttg aacatctttc acgaatgcaa
    11341 tatgcaggat tcaatgccga aatgtagcgc catgtgcgat ataattgggt cataatccta
    11401 cattatgaac ggattaggca caggccgaat cttggaggta gcaacgacct gccaaaacga
    11461 cataccccct catacgcctt gacctccgca ccaagcccag gcatccggct gccatgatcc
    11521 ccaggtatac cccgtcggag ttcgcaatac tgtggtcgga tgaaaccagg tttggcatct
    11581 ggttggacat cgaattggca gcttgcgaag ccatggagtc cgaaggcctc gttcccatcg
    11641 gtacggccaa gaccattcgg aacctgaatc tcgtgctcga tccccaacgc atccacgaaa
    11701 tcgagaaaac cacacgccac gatgtgatcg ctttccttac gcacgtcgaa cagctcgccg
    11761 gcgctcccgc tcgctacctt caccgcggga tgacttccag cgatgtgctc gataccggtt
    11821 ttgcgattct tctcacacgg gccaccgatg cgttgctcct tcgcctcgaa tcactgctcg
    11881 aagtcctgaa gcgtcgctcc aaagagcata gccacacggt actcatggga cgttcccacg
    11941 gtattcacgc ggaacccgtc acctttgggg tggtgcttgc tgggcattat tgcgaattga
    12001 aaagggggtt ggcgcggctt atggtcagcc gtgaggagat tcgaacgggc aaattggctg
    12061 gcgcggtggg tacctacgcg catctttctc ctgcgatcga acgcaaagcc ctcgagtctc
    12121 tcgggctcaa agccgaaacc gtgccgaccc aagtggtggc gcgggatcgt catgccgccc
    12181 tattcggagc catggcgttg attgccgctg gtatcgagcg tttggctacc aacgtgcggc
    12241 attggcagcg cacggaagtc tccgaagcgg aagaattgtt ttccacggga caaaagggat
    12301 cgagcgcgat gccccataaa cgcaatccga tcttgagcga gaatttatgc ggcctatcgc
    12361 gaatcgttcg cgcggccgtc actcccgcgc tcgaaaacat cgtgctttgg cacgagcgcg
    12421 atatctccca ttcatcggta gaacgaatga tcgcgccaga tgtaaccacc accctggcgt
    12481 tcatgctcga tcgggccacg caactcatcg aagggctggt cgtctacccc gagcaaatga
    12541 atcgcaacgt gcagcgatcg gccggattgt gcttcagtga aagcgtgctg ctcgcgctcg
    12601 tcgaccgggg gatcgcacgc caacaagcct atgtcatggt gcagcgcaac gccatgaaag
    12661 ccatcgaagg acacggctcc ttgctagaaa accttctgtc cgacgagcag gttgtcgaac
    12721 gcatcggaag agagcctctc attcgctgtt tcgatttgga gcactcgctt cgccatgtcg
    12781 atgagatatt ggctcgtgcc ttcgaatgat cgatggggat cgtagtcgga ttcgccaggc
    12841 gccgcgcgca atgagacagg cgatcacaaa ggcatgtctt ggcttgagct cacttcgctc
    12901 cttgtcgtag accggacgaa cagacacttc ggccacgcgc aagtcataca tacgaagctg
    12961 aaacagcagg tcattgggat aaccgaacga aggccacaga ccgtgcagat cgagctttcg
    13021 tatggcgttg gaagaaatcg ccgtatatcc gcattgacta tccgttaacg gatagccaac
    13081 cacctggctc gtggcccaac caaagacgcg cgttccgagt cgtcgcaagg caggcatgcg
    13141 gtacgcctcc tcatgggata agcgggttcc tttgacataa tcggctttcc catcgaccac
    13201 ggggcgtagc acggccagca gatcatccgg atccatttgg ccatcgcctg ccatcaccgc
    13261 gacgacgtga gcattggcct gaagggcgag acgatacccc gtggcaatgc tggctcctac
    13321 gccacggttg aaagcatggc gcaggatgtg aacacgagcg tcgccatgct gctgagccac
    13381 acggaaggta tgatccgagc tggcatcatc gacgacgaag atgaagtcga cgtaggcggg
    13441 gatggtatcg aggacgcgat ggatgaagtg ttcttcatca tgagcgggca ctacgaccgc
    13501 aatgcaggcg ccatcgagca tgggcggggg gggagggtta ttcctcttct ttttcgggta
    13561 gttcagcttt cggtgtgtcg ccggattcga aacgtgcgga atgatgttct tcgcgttttc
    13621 gggtcacgag ggcgaggaca cgcccgagca ggtcggggat ttgttcgaag agttgtccga
    13681 ccatttgttc gggttcacca tcgatggcca tgagacgagg tttgccgtct ttggcgatgg
    13741 ccacgacagc gatgggttcg acttggacgc caccgcccac accgcgtcct ccgtaattgc
    13801 cttcggtggc ttccccatgt ccaccggcgc cgatggcggc cacgccgaaa ccgacgcgca
    13861 agcggtggat ggggaccagc atggcgtctc ccgcttgcaa aggttccccc accacggttt
    13921 caatctttga aatctgatgc attccatcga gaagatggtc aacgacttga tcgagcttac
    13981 cggacatgat gcgctcccat cgtcatggcg gcgcgggcac ggatcgagta ttttcgcgct
    14041 gccgcagctt gatttttttg aaggtgaata cgatggcgtc cagcaccatc ctgccgggat
    14101 agaaccgaaa cgccaactcg aggttgcccg aagcttttgc gacatccacc cacaacggtt
    14161 cgatgtgaaa gcttccgaaa ggggcaaata atcccgcgaa agaatagagg gaacccaaaa
    14221 ggattcctgt catcgccaaa tcgggcgtgg cataggacaa ctgaccgttg agccgttcca
    14281 ttcggacgcg atgacgcaag tccactgcga acaccaacag gtcgtccaga tcgaaccacc
    14341 gttcgacttc gcgacgcaaa cgtcgtgctt tttgcaacat cacctcgagc gatggtgaag
    14401 ccggctcgct cgttggacct ttgcgtttga cgggcgagga tcggaacaat cgccggccga
    14461 acaggtgaag ttgaaagacc gagtcgatac cgtaggccat ggcaaacgaa accgtaaaga
    14521 acagcagttg gccgcccatg gcaacggccc agaattttcc gaactcgccc tgacctttta
    14581 tggatacttc gagtcgcacg ggtgccacga tcaccacggc caaggtgatg aagcaaacga
    14641 cggcgacggc gagcactacc cacagcacga gttcctacgg ttcctgcgtt cgtgactatt
    14701 tgtcatcatc ctttgcggga aggacttggt tgacggtttc gggcagcacg tcgatggcgc
    14761 gctgaaggat cgcgcgggcg cctcttcgca cggacagcat ttgcgcgtgg ccaccttttc
    14821 ccacgacgac gaaggcacgg ggttcgacgg acaagccgcc tcccgcgcca ccgacatcga
    14881 cggctccacc ctttttcctc atttcgccag aaacgtcgcc cgaaccggtt ccgaatccca
    14941 cggaaatccg gcataggggc acgacagccg aatcagcaaa agacatgggc ttacccacca
    15001 ctgattcgga gttggagacc cggtacaatt gtttcatcaa accgctaaca agatcgtcga
    15061 gttccatcaa tatcctccga taggcctcgt gcgttgttga aagacacgtt gcgaagcaaa
    15121 aaaacaccca cttgttggtt cgttgtgttt tcgaaccctc aagagcaggc agtatggagc
    15181 aattcgctcg ctagtggtag aggaaccaac gaacatcagg ggtcaggtcc atgtggaagt
    15241 ggtcctgatg ggcttgatcg aagttgggtg tcagtacgta cgtgaagatt ttggtgtcgt
    15301 aggcttcaca tacaatatcc cacagttcac gtgctttcgg ctcttgcgga ggcgcaacac
    15361 ccggtccaca ggtttgagcg ccgatttgac cacgaaagtg cgaagccaca ctgagcatgg
    15421 tgccgtcttg cttgatgaaa atacccgcat cgatcgctag tcccaaggga tgacgcgggg
    15481 ggctgacgtt caaccgtggc gtggaagagg cgaggtgggg tggctttttc gtgcgggaac
    15541 cggcttgagc actggccgcg ggccgaggtg gttttttttt cttggaggat tgatgggggc
    15601 tttttttggc ctggacatac cacgactccg gaggcccaac cttggaagcc tccccaaacc
    15661 acgcctggga agaggccgcc ggagaagcca ccgaaggtcg atacatcgtg taatgaacca
    15721 attccacgat accccgtcga gcaagcaacg cggaaaaatc gtcgagggcg agagccaacc
    15781 gcgcatcaca gatttcaaag ggagtggtcg gtcgttcatg ctccgctagc accgaatgaa
    15841 tatgaacgcc gtgcaatctg cccgtcaggc gaatcggcgt tcgtaccgca ccatgaggct
    15901 ccacacgctg gtaaaggatc ttgcggcgat ccagttcgtc gagtgcctct tgttccgaag
    15961 cgttggcgta tcggtacgcg ggagatcgct ccaccacttg cgcggccgga acgaccaaaa
    16021 acggatcact ggcttgcgcc aaccaaggcg ccacgacgaa aaccagactc gcggacacca
    16081 atacccaagg ccaggtcttc gtttgcctcg tttgcatggc atgccaacct agcgcacgaa
    16141 ctgccgttgg ccaggattcc gcaaccatga cctgacgtat ccgaaaagga tgcgaaggac
    16201 actgcccgct actcactccg cctggacacg ccccccaata ccgcgaatca tcatgccgaa
    16261 tgccggatgc cgaaaagcaa aaggctgatg atggtcctat gcgccagagt caggagctgc
    16321 aagactcatt gcccgaaagg tggaggagac aatgaccagc gatcgattca aagagatggt
    16381 cgaattcgcc attttgcgtg agcaagaagc cgtggatttc tatacggcca tggcagacaa
    16441 cgtatcggaa acacacatta aaaacgtgct tcgggacttt gccagccaag agcgcggcca
    16501 caaagcaaaa ctacaatcga tcctggatgg gaaaaacccc accctggtcc acaaggacgt
    16561 ggccgatctc aaaatcgctg attacgtcgt cgatgtcgag ccaacccaag acttgtcttt
    16621 tcaaggcgcc ctcatcatcg ccatgaaaaa agaaaaggcg gcttatcgtt tgtattcgga
    16681 tatggcgacg gcttgtgacg atccggtcgt tcgcgacttg ttcctgtggc tcgccaacga
    16741 agaagcatcg cacaagctca agttcgaaat cttgtacgat gatttgatct tccgagagaa
    16801 ctaatgggtc atccgtcggg atacattccc tttttgatac cctcgagttt ctcgaggaga
    16861 tgatcgtgct tttgctcatc gaacagcagg tattcgagca agtattgctg cacgagcatt
    16921 ttccggcctt gtagcgaatc caacgctttg cgaacgttcg ccaccatgtg ttgttccgtc
    16981 ttgatgtgct cctcgatgat gtcccacacc ttggcgagat catcgggaga cagggcgtga
    17041 gcgccttttt cgatcgagtc caccaccatc tgctgaactt tcgcgtgtcg caacgaatcg
    17101 gcgtgaatca cgtccgccac cagcttgatg agttcgtggt tcgtggcttt ggagagtttc
    17161 tccatctgat tggccgccgc cccctccgct ttctgccagt tcttcaggtc caccaccaat
    17221 tgctcttgca gttctttcgt cgagacgttc atgcattcct ccgtccttcg ccaaggcatt
    17281 gccaccccgg cgcgtttcgt ttgccacgcc agggcggttg cttcccggcg ccatccattc
    17341 accactcgct tcgatgcagc catgctgcga tccgtttccc cattcatgac aacgattacg
    17401 gaaacgcgag aattcgcccc tcgatcgctc gaccccctcg gccagaattc cctatggatt
    17461 tcccttactt ataagcatgt ccatcatcga aatgagtttg cggacttcgt cgggacgcaa
    17521 agacacccgc gcctcgacca tctcttgttc ggcacgaaac accacgggac ggaacaaatg
    17581 aatacgaacg accgatacct ttaccgtcgt caaacgttcc accaaccgcg tcagctcttc
    17641 cgcgtccgtc acggcttgtt ccgctgtcgt actgcgagca ttggcgacaa ggacagcgcc
    17701 accatcgtcg acgagccgca cccgaacctg ggcttcgctt agcgttgcgg gaatcccgcg
    17761 cacatagggc cgaagggctg tcgatgggtt ttgcatgctc accacaagat catcctgact
    17821 atccgaaggc ggatacatca gcggtccgtc gaaaaccttt gcagtggcga tgcgttcgct
    17881 ttgcagcacg atgagcaagt ccggatccgc aagcacgagc cccaccggtt cggatcgaat
    17941 atggatacgc gcgacgcgaa ccgtggattt gttcaaccac tgcccaggag gatcgcttcg
    18001 ttcgatggct ttttcgatcg cctcgttcaa tcgctcgggc gtcatggaat gacgaatgac
    18061 aaggatcgcc tcatcttgcc gggataatcc ggtcgaagcc aagaacagcg aggagatgtc
    18121 attttgcgga tcgaggtcgg tgctttcgag aatcgacacc acgtcgggca ggcgaattaa
    18181 ccgttttcca agaggatggt gtagcaagcg ttccatttcg agggaggccg tgactgccgt
    18241 tgggatcaac gaacgcgcaa tttcggtggc atctttgggt gtgggcggtg cgggtacggc
    18301 aggtggggga gctgcaggat cggtttggac ggatgggtcg ggcgattgag cgatgggggt
    18361 ttttgcacag ccgatggaga tgatgacagc gacggcgagc ccccatcgcc atgccgcgga
    18421 aaccaaaaag gaacgcgaat gggtcataaa gcctctcgaa aacactgttc gagcgcctga
    18481 tcggacaaga ccaccgggtt gtagcgcatg gagcttgctt ttttggcggc ggccacgaca
    18541 gcgggcgtgt cagaggcgga tacgccgaag gacgagaggg ttggaacctg aagtttatgg
    18601 acgagatccc gcacgaaatc gacggtggca tcgacggctt cctggggcgt ggcaaatcgt
    18661 ttttctgtaa gaatttcgcc aagttgcgca aaacgggcaa gggtaggatg ggccctatcg
    18721 agttgtctca acgctcgcac gttggcctcg atacctggaa cgagcaaggc cgcgcaggcc
    18781 gcaccgtggg gtatgggaaa catcgcgcca aggggtgcgg caaaaccatg cacgactccc
    18841 aatcccgcat gggcaagaca gacacccccc atgagggaag ccaaggccat atcatcccgc
    18901 gccgcctggt cggagccatt ttcccaagct ttgggcaacg cgcgggcggc cagcttcacc
    18961 ccttggagcg aaatcccgtc cgtccaagga ttggcacccg tggatacgaa aggctcgatg
    19021 agttgagtaa gcgcgtccaa tcccgtggat gcggtcacgc ggggaggcac ggaatccgtc
    19081 aactgcgggt caaccagcgc cacgcgagct atcagatgaa cgctgcgcat cgaggctttg
    19141 aagcgcgagg atttgtgcgt aagtaccgca ttgcgcgtca cttcggtccc agttcccgcc
    19201 gtggtgggaa tggcgatcat cggcgcacaa gccgctgcaa tgggtttgcc acgtccgacg
    19261 acttccaggt aatcgagcgc ttccccaccc aaagacagca gcccggatac cgctttcgca
    19321 gcatcgagaa cagcgcctcc tccaagaccg accaccatgt cacaggcgtg ttttcgagcc
    19381 agttgcgtgg ccgtatccac cgcgatggtg tctggctctc ccacgaaggt gaattcgaca
    19441 caatcaatac ccacggcgtt gagttgagac cgtactcttt cgagggttcc aaaacgctcg
    19501 agatgactcc ctccacgaac cagcgccgct ctggttcccc acggagccgc aagttcccca
    19561 agacgatcga tggcgcctct tgcgaatacg actcgtgccg gaagacacac ctcgagtttc
    19621 atcgcttacc actccgtatc atcgggaaac aggctgtgat gacgcacgcc ctggcgtggt
    19681 tgactcatcc actccgtcac ggtttgtttc cacgtggtgt aatgttgcgt tttctgatga
    19741 gcgacgaagt cctcctccgt ccgatacgct tcataaagaa aaaaacgcgt gggctcatcg
    19801 gcctttcgaa gcacgtcgaa gcgcaaattc cccggctctt ttcgagtacc cacgtgattg
    19861 tgccgagtgg cctcgatgaa tgcttcttcg aatcccggtt tgacccagat cgttacgcaa
    19921 accacgtaca tgggcgacct ccttacccca aggcatagcc aagcaacacg ctggcgacca
    19981 gcgtcatgtc ggacaaaccc catcgaaccg ctcgaccgca ccgtcaccat gaatcacggc
    20041 gccgtggctg atgaggagag cagataaggg gtgtcatgac cgcatcacat gtcttataac
    20101 ctactgatat tattgtatta tttgtattcg tagcagcatc gctgcccacc gaggagtctt
    20161 cgacgcacgc ataagctccc gctcatggtg aatcggttta ttctgggtga gtcatgccca
    20221 attccccgtt cccaggacga tgccctcaat gccaaggccc tttgggtcaa tacgccgact
    20281 catgtccatt ttgtggcacg aatctgacag ggcttcgaag cgaggcgccc cagggacctg
    20341 cgttgcaact cgacgctccc ttgcatcccg tggccgcggc gcgcccacga cccattcgtc
    20401 ctcgcccagc gacacccaaa ccaggcatcg tggctcgcat gggagtcagc gtcgtggcgg
    20461 gagcggctct gttggtgctt gccctccttg cggcgagcgc attttttttc cttcgagcga
    20521 cctccaagcc cataccgctc gaggctccgc ctgtcgtatc gtccacacca tcgccgccgc
    20581 cgctcaacat ccacggcatc gtgttggcgg atggtcctcg gttcgatccg acggacgtgt
    20641 tgcccatgat tcgcgagcgg attgtggatc cgggcgatcg gtccgaggtt catttgcttg
    20701 ggatcgttgt tcgaggggcc tccgaaggag ctgtgaatct cgacagccag ggagcatcgg
    20761 tcacatatca atatttggtg gtgcatcgcg atcctcgtgc ggaaaaagca ttggagcgca
    20821 agggcgagcg tgtcgaaatg acgcttcagc gggacccacc gccgatcaca agggtggaag
    20881 aaaacccttc caccaagacc gttaccgaac cgttgtgtgt atggcacgcg gcatggcaag
    20941 cggctgtggc atccggactg ccacgaacgg aaatcatgga tgccgtttac gcgttggatg
    21001 cacagggcaa acacggggta tggtcttttt cccttcgcaa tcaacctgag tcgaaccgtt
    21061 ggatcgacgg tcagacgtgt gcgatcaagg cttcgcagcc agcgcaggtc aatccgccac
    21121 cgtagcggca tccgttcatt ttcacaaagc gatggccttg cttcgtcatc gctggaacgc
    21181 caggatggcg aggtgacacg tccacgggat ttcggctttc gcatcgagtg ttttccgact
    21241 cggaccagga ctttgcctcc cgcgacccat acgaatgcct atgctctcgg cggaagcgaa
    21301 gtcttgatcg tggaacccgc gccagaggat aagaccgagc agaaagcgat gatcgactgg
    21361 gtgcaagcca tgcgaagggt tggtcgtcgc cccctcgcca tcgtgatcac gcatcatcat
    21421 cccgaccata tcggcgcggc cgcggtgatg caaaaagagc ttggtttgcc gctctgggcc
    21481 catgcagcca cgcaagcgct gatgccccat ctatcgattg acaagagcct cgtcgacggc
    21541 gaccgaatcg tgctcgatga tccgttgcaa acatgggagg tattgcatac gccgggtcat
    21601 gcgtcgggtc atctttgcct attcgagcgt gacctgggtg tgttgttgct tggggatatg
    21661 gttgccacga aaagtacgat cctgatagag ccgagcgatg gggatatgcg tgagtatctg
    21721 aagcaattag agcgtttggc agctttggag gctcgcgtgg gtcttcctgc gcatggggaa
    21781 cccatcgagc aaccttcgcg ctttcttcgc gctacccatg cccatcgaat gatgcgagaa
    21841 aacaagatca tcgatgtgtt gcggtcgaca gccgctttcg ttgcgctcga tgaaatgctt
    21901 gctttggttt acgacgatac atccgtggta ctttggccct tggcgaaaaa gtcgttgctg
    21961 gctcatttgg aaaaactgca agccgagggc aaggttcatg gcaacaacgc gggatattac
    22021 ggctgggtag gcgagacata gaaaaagcga acgacgcttc acctcatgct agggatttgg
    22081 gatggagcgt gagccacgat gagcggtcat gacatgggat tgatgagtca gtgcaacgag
    22141 gatcacgagg cggcgggagt tgccgcggcg acactctatc tattggaaaa ttcaatagat
    22201 tttttggaaa cgcataagca ggatccggca aatctcaaac cgatcgaaga tctgattcgc
    22261 atgctgcgtg acacggaccg ggatcgtcga gcccgagcgc tagccgccta cttgggaaag
    22321 ctgtcggtac aactcgaaag ctggagcctt cgatgacacc aggacgggga cgcatcgtgc
    22381 gtgacggacc atcgatgact tcgctgactc tttcatcgct tccgacgttg cccgcatgcg
    22441 gtatggttgc ccaaggggcg cgcggaagaa tcgttcgacg agagcggttg gagatttcgg
    22501 aagaacggtc gggaatactt gccgtcgcgc ggcaacaagc acaggagttg atgcaacagg
    22561 cgcaacgaga tgccgaggaa gttcgtcgag aagccgaaca gcgaggagtc gcggatgggg
    22621 ctgccaagct ggccgctgcc tggttgaacc tcgagcggcg tgaagcaact ctcgacgtcg
    22681 agtccacgga aagaacgatc gccattgctc gattgcttgc agaaaggctg ctgggaaaat
    22741 cgttggagct tgatccggag ttgatcgccg acatggcaaa agaggcgatg gttcatctct
    22801 ggaggtccca tcgggtggtc atccatgccc atccacacga tgtgcccatg ttgacgaagc
    22861 acgtcacaac gtttggaatg cctccggaac gaatcgagat cagggccgat gacagcagac
    22921 aacgaggaag cttgcggttc atgagtgatt tgggggaatt ggatggcgag ttggcgccgc
    22981 aactcgatcg gctcgtcgag gttatccgac aggagttggg aaatcgaatc tatccatgag
    23041 tacaccgcac tttcctccgc cagcgcccga cgtccagcgt tcggtattcg aaggcgcatc
    23101 cccggagccc gtttcgggcg tacctcccga gcctcctcgt cggaatcgtt atgcgataca
    23161 tctcggcctg tttgtcgcca ccgtgatcag cgtgcttttc gccggcaatc aagagctatt
    23221 gctcggcgat gaacccctga cactggcgaa tctatggaag ggatggacgt tcgccgtacc
    23281 gctgatggtg atcttgctcg cgcatgaatt tggacactac atcgcggcac gttggcatcg
    23341 cgttcatgcc tctttgccat atttcattcc catgccgatc agtcctttcg gaacgatggg
    23401 agccatcatt tccatgaaag gacgcattgg ttctcgaaaa gccttgctcg atatcggagc
    23461 ctccggaccg ttggcgggca tggtcttcgc cgtgcccatc ctcgtttggg gtttgctgca
    23521 ttccgaggtg aaggagatca cgggttcggg gctgctcgaa gggcaatgcc tgctgtattc
    23581 cttgttgaaa cgattgaccg tgggacccat tccggaaggg agcgacgtat tccttcatcc
    23641 cacggcgttc gcgggatggg ttggactgtt gatcaccatg atcaatctgg tgcccgtggg
    23701 tcaactcgat ggaggtcatg tggcgtacgc gctcattggt cctcgtcaaa acgcgatcgc
    23761 gcgtatggtg cattatggct tactagctgt atttgcagtg aattttggtt atcacttcct
    23821 acgccatttc cagacggaac cccttgggca aaccgtgggt cgcgccgcgg agatgtcgat
    23881 gttttggttg gtgtggtttg ggtttctctt tctgcttcga gccttgtccg gagggaatca
    23941 ccctcccacg gagccaggag aattgagccg gggacgaaaa gccatcgcca ttggcacttt
    24001 gctactgttc gtgttgctct tcatgcccac gcctctttcg cagtattgaa tggcaagata
    24061 acgacagcac gacaagcgag acttgccacc tggctgtcgt ggtccccaac tcgtttcgcg
    24121 catggattcc cacggctgga tgcgccaacg ggcggccatg ctacgctcca ctcatcttat
    24181 tcttatcgcc acgccaaggg gcatggggtc gaggttcgtt atgaaagtgt attcttgtca
    24241 tcgcgcttgc ttgtctgctt gtctggggct cagcttgtgc atcccctttt tcggatgcac
    24301 gtcttcggat ccaaacccga tggatgtgcc cccggcgatt cccggcgagg cttgtacacc
    24361 ggctagccaa cgatgctctt cgcaacatga cgggagtgtg gagatatgcc gggccgacgg
    24421 tcgcggctgg ttttcgcaaa tgtgtccgac ggggacaacg tgcaacgtta ccaccgggtc
    24481 ttgcgaaagc gtggatacgg gatgcgaacc aggctccgcc cgttgcgaag gaagtccagg
    24541 aaccgccgct cgtgcggtat gcgcggccga tggaaaaaga tgggcgctcg aaccttgtgc
    24601 gggcttgcaa gtttgcgacg cctcgagcgg ggcatgcgtg gatccggttt gcgcctccgg
    24661 agaatctcgt tgtgaaaaag gtccgacgga tcccgatcgc tcgacgtgcg cacccgatcg
    24721 actctcgttc atcaacacac catgcccagg caatcacgtc tgtgttccca caaccactgg
    24781 ttccacatgc gtggcacgcc tgtgctcacc gggcgacagg caatgcagca acgattacct
    24841 tcaagtcctc gtttgcgata ccagcgggac gtcgttagag atcatcgaga cctgcgccga
    24901 cgacgaagct tgttccaaag gtatttgtga aaaatccacc atgccccttg gacaaacggt
    24961 ccgcgtgacg agcagcgatg gcccgttttc cgtgagtcca ggcagatacg ccgtggtcgt
    25021 ggtcaatacg gacacgtcca acgatgaatt cgttgactat ccgctgcacc tcgatggttc
    25081 catctcggat cccgtcacta ccacctttcg tcatctcgaa ccgcaaaagg tctccgatcc
    25141 cgcggccgtg tcgcgaggtt atcccatgag caaaggcaga acggccacgg tcttcccacg
    25201 gcctgggccg gaacctaacg atgcggaaac acgtactttt tttgttcctc gctatgaaac
    25261 cggaggggaa gaaaccatcc cgcgcacggc aacactgcgt gcctccggtc aacacgtgcg
    25321 ggtgtgggaa gaccagagca cctcttccgc cgggacattg gtccccgaca ccgtgttgga
    25381 cgagttgatg gatcgtctcg accatgccgt gcttccccgc gcgaaggcca tggtgggcga
    25441 gccgacggat gtggatggca atggaaagat tgatatttta ttcacggacg tgctccccga
    25501 tgctgtcaaa gcgttctgtc atccttccgc cacgctcttt gcgccgggga cattttccgt
    25561 gtcctatgat tatggagagg tcgtttatgc ccacggtatc gatgagtctg cgcccgtgtg
    25621 ggaaacatcc accttgatgt cgcatgaggt ggcacagctc atcgtgttga gtcagcggct
    25681 ggagccgttt ttgtcggatc cggaatcgat tcccccctgg gtcgaagcgg atatatacgc
    25741 ggtggaggga ttggctagcc tggtgatggg ctggagtggg caatcgcatg catggccggt
    25801 gacaaccgcc ctcgaacacc cgcaggaagc gtcgttggcg cgcttgacgg cctcgtcgta
    25861 tctcgcggac gccaccgcca acctggcatc gtacggattc ggaaccctcg tgcaggagta
    25921 tctattcgac caggcagggg cgatggtggt gagcgatggg ggcgcgacca tccaagatgg
    25981 tggaggaaag gcttatctac aagcattcac cgcgggacaa gaaggatggg atcggatcca
    26041 ccctttggat gggcgaccgg tttctacctg gtacgtggac tttgccacgg cgctacttct
    26101 ggttcacctg gatggaaagc ttgcgccatc gacagccaag gatccacgaa cggcatttgc
    26161 ccccacggtg ctggatacga aatatggagg cttccttggt cccaccctgc gatgggagca
    26221 tgtgcgggac tcgtcgaaaa aaggaccgat tcatcgcctc atgccatggt cacaacaccc
    26281 cgcctcgatg cgccggggag gcttgagttt ccttgcggta agcgtgggag acagcggtgc
    26341 gacgatgcag gtatcggagg acgcgacgac cgcggtatgg gtgcggcacc gaccttgagg
    26401 gcgagaggtt atttggcggt gatatagcct tcgtgggtga gccattccgc ggtcagcacg
    26461 gcgccgccgg cggctcctcg gacggtgtta tgggataggg caacgaaacg aatgtcgaag
    26521 atggaatcgg tccgaaggcg tcccacggag atgcccatgc ctttgccgag gtcgcggtcg
    26581 agtctggtct gcggccgtgc atcttgttcg aagtaggtga gccaggggca gggagccgtg
    26641 ggcaatccca gccgttgggg caaactggtg ccgttccgcc atcgagccag gatttcctcg
    26701 cgacaagggc gtttttcgaa agccacgaac acagtggcga ggtgtccatc ggtcacggga
    26761 attcgtatgc attgggcgct gatcacgggt tgcgcgctgg ggacgatgac cccgtgggcg
    26821 aggcgtcccc agatttttct gggttcttgc tcggactttt cttcttcgcc agcgatgaaa
    26881 gggatgacgt tgtcttgcat ctccggccag gattcgaacg ttttaccggc gccactgatg
    26941 gcctgatagg tgctcacgac cacatgccga gggcgaaagt ccatcagcgg atgaatcgcg
    27001 ggcacatagc tttgcaccga acaattgggt ttcaccgcga tgaacccgcg cgtcgtgccc
    27061 aatcgctttc gctgatcggg aatgacgttg gcgtgttccg gattgacttc agggatgatc
    27121 atgggcacat cgggtgtggt gcgatgagcc gaagtatttg aaattacagg agtttctgct
    27181 tttgcgtacg cttcttcgag acgcgcgatc gcgtctttgg gcatgtccac cgcgcaaaac
    27241 acgaagtcca cctcatcggc gatctggctg acgctggagg cgtccgcgac aggcaaggaa
    27301 gcgaaagacg ggggaagggg tgatggcaac gcccaacgat cgtggacggc ctcggcatac
    27361 gataagccag cggaacgccc gctggcggca accagcgtca cgtcgaacca cggatgatgt
    27421 tcgagcaagg ccaggaatcg ttggccgacc atacccgtgg caccgaggac agcgacgcgc
    27481 agctttttgg gaagctcgat catggtgcgg cggaagctat caccgtgcga aagaacgggc
    27541 caatccgttt gagaaaagaa ggggataggc gattacggag cggtatccgg acgccatgcc
    27601 gggcgcgggt ttttccacca ttgttctccc gcgagacgat gaacggtgag gcgaagcaaa
    27661 tcgataccga acgtgattcc aaaagtttta tggtcgtcgg accccaaggc gccgttgagc
    27721 gagagttgca acccgtaggg ggcgccgagt tgtaaggagg ccatggggcc ggctgattcc
    27781 atgccccacc aacgtttgta agcaccggcg tcgagggcga gaccaaaacc gccgctcgaa
    27841 aactcccgcg tggtggcccg caacgcaagg gcaaggtcgg ttccacgacc aaaatgcgtg
    27901 agcgaacggg tcaatacgcc aaggacaacc ggtcccgacg gtggcgttcc gagcccgaga
    27961 tcgagctgca tcgccggagc gatctcatag ccgctcttct gttcgctcag ccagtgcgcc
    28021 cccccaccca cgaaaagcca ggaggaaacg tccgcatggg cagacccagc ccccatcatc
    28081 accatggaga tcgtggcaac ctgtaatagc cgagacgtgc gggtcatgcg ggacacgtcg
    28141 gagctagcac gtctgtgcga cggatgcgac atgagttcgt cctgctctca aagccaatgg
    28201 tgttcgcggt accaggcaag ggttgcggga atcccttgtt cgatggtggt atttgaggtc
    28261 cagcccaagg cccgcagcgc atcgcttttg caggtccacg cgggctgcgc gcccatcttg
    28321 gccttttgac ggttgaacag gcgcggtttg tggtcgagcc tggtcatcca ttcccctccc
    28381 aaggccgcga tatccacaat gatttccgga agatcgagcg tgcgcacgcg ctttccggaa
    28441 gccttgacga tctgctgttg aaaacgctgc cacgtcacgg gaacgccatc gcaaacgaaa
    28501 taccccttgc ctaccgtgtc cggatgctgc gcggagaaaa ggagggcaga gacgaaatca
    28561 tcaacgtaaa tacctgaaaa cacacggttt ttatttccga agtacacatc gaaaccgcgt
    28621 tcgatctgtt tgaataattc gaagtaatcc acatcgccgg ggccgtacac accgccaggc
    28681 cgcaagatgg tccagggcac accgtgttcc gcttcgacca tgcgctcggc ttcgagtttg
    28741 ctctccccgt agtattcgat ggggcgtcgc gggctcgatt cgacatgagg ctggtccggg
    28801 gcggaggggc cgtagctggt caatgacgag acatgaacga atcgttggac cgcgggatgt
    28861 tcgtcacgca ccgtttcgag cagatttctc gtaggcatga cgtttccgtt gcggaaatcg
    28921 tcgtaggtaa cgcctttggt gacgccggcg acgtgaaaga cccaatctgg tttttcgtgg
    28981 cggatcatgc gcgcgatgcc gtcgcggtca tcgtacgata attcgataga gcggcctcgt
    29041 ttcgctggag ggctgccttt acgtcgtacg gcgatgacat cgacgccttg gtcgatcagg
    29101 gtgtcgcgca atcgtccccc aatgaatccg ctagcgccgg tgatgagggc ttttccttcg
    29161 agacgtggag tatccatcga ggcgcgtcat agcatcaccg ctgtcggtga acacctccaa
    29221 gaacggggat tcgaccgtgg caggtgtatc gtcggcggtt gacgctcgaa gggtcgagcc
    29281 ccagggagcg gacatcttcg ggacaggaga aacggcagtg tcttatcacg acaatatttt
    29341 acgcgcgatt ggccgcacgc cgctggttcg gcttagtcga atggtagggc cgcgcgacgc
    29401 cacggttttg gtcaagccgg aatacatgaa tccggggggt tcgatcaaag accgtatggc
    29461 gttgtatatt ttggagaagg ccgagcggga aggaaagcta cggcccggcg gcgtcatcgt
    29521 cgaaaatacg tcgggcaaca cgggcgttgg gctcgccatg gctgccgcgc tcaaaggata
    29581 tcggtgtgta ttcaccattc ccgacaaaat gagcgcggaa aaggtcaaca cgctcaaagc
    29641 gtttggagcg gaagttcata tctgccccac gaacgttccc gcggatcatc cagacagtta
    29701 ttacgagacg gccaaacgat tggtgggcga gcttggcgcg tttttcccga atcaatacca
    29761 caatcccgtc aacatcgagg cgcactacca caccacgggt cccgagattc tcgatcaaac
    29821 cgagggaaat attgattatt tcgtcgcggg tctgggcacc ggcggcacga tgagtggcgc
    29881 gggtaagttt ctcaaagaca aagtgcccac cctcaaaaac gtaggcgtgg atccggaagg
    29941 cagcgtgtat cacagcctat ttcacaccgg aaagctttcc acaccgcatg tctacaaggt
    30001 ggaaggcatc ggcgaggata tggcgtgcgc tgctctcgat ctgtcggttc tcgacgatgt
    30061 tcggcaagtc aacgatcggc aatgtttcat cgcggcgcgg cgtcttgcgc gcgaggaagg
    30121 tattttcggc ggaggatcgg cgggagccgc cgtgcatgtg gctctgcaaa tcgcaaaaga
    30181 agcaggtccg gggaaggttg tggtggcgat ccttcccgat gggggacgcg catatatttc
    30241 gaagttttat tcggacgaat ggatgcggga catgggcttt ctcgaaccgg atccctcggt
    30301 ggcccgcgtg gagcatctgc tgcaggacaa accgccggtt gtcgtggcga ccgtgggaga
    30361 gtccacgcgg caggttgtcc tgcgaatgca aaaacacggc ttttcccagc tacctatcgt
    30421 caatgggcaa gggcgcgccg tgggcatggt ccatgagtcc gacgtgcttg gttccttgct
    30481 ttcgggcgat cgaaatctcg atcagccgat cgaacctgcc gcgctgcaag gcgtcgtatc
    30541 gctacgcaca ccgattcatg aagtcgaaaa agtattggcc gacgataacg tcgcggtcgt
    30601 gatcgaggac gggcgcgtca tcggcatcgt caccaagatc gatttgatca cccatcttgc
    30661 caacccaccg cgatgaaaga ggtcatcatg cgattcgaga ccaacgccat ccatgcggga
    30721 caatcgcccg atccgaccac gggagccatc atgacgcccg ttttccagac ctcgaccttc
    30781 gtgcaggaag caccgggaaa acataaggga tacgagtatt ctcgaacgca gaatccaacg
    30841 cgcgccgcgc tcgaagcctg cgtggccgct ctcgaaggcg gtcgccacgg ccttgctttc
    30901 agctccggtt gcgccaccac cgatgcggtc atgcatctgt tgtcgtcggg cgatcacgtg
    30961 gtgagcatgg acgacgtcta cggtggaacc cgacgattat tcgaaaaagt gtgggcacgc
    31021 cacggcatcg attttacgtt tgcggatctg tccagaccgg gcgaactcga ggcagcgctg
    31081 actgatcgaa cgcgattggt gtgggtcgag tcccctacca atcccttgct caaactcgtc
    31141 gatctcgagg ccaccgcgcg gtcctgcaag cagcgcggca tccctctggt cgtcgacaac
    31201 accttcatga ctccgtattt tcaaaagccg ctcgcccttg gcgcggatat cgtggttcat
    31261 agcgcgacga agtacctgaa cggtcattcc gacgtggtgg gaggtctgct ggttacgaac
    31321 gatgaggcgc ttcgtgatcg gcttgcgttc atccagaact cggcgggcgc cattttggga
    31381 ccgatggatt catttttggt gctgcggggg cttaagactc ttcatgtgcg catggatcgc
    31441 catcaatcca acgcgctgga attggcgacg tggctggaga ctcatccccg cgtaaaaatc
    31501 gttatctatc cagggcttac atcgcatccg caacatgaac tcgctcgcaa acagatgagc
    31561 ggatttggtg gcatgatcac gttgcgcctc gacgccaatc tcgagcaagc cgcccgcgtg
    31621 atttcatcgt gtcgggtgtt ttccctcgcc gaatcgctgg ggggagtcga aagtttgatc
    31681 gagcatccag cgatcatgac gcatgcttcg gtggcgccgg aagagcgtgc gaagctcggg
    31741 atcgatgatg ggctcattcg gctatcggtc ggaatcgaag cggtcgaaga tcttcgtgag
    31801 gacctggagc gggcgtttcg ggattgatgg gagcaagggt tgctgcttgt tttgccgggt
    31861 ggtccacggg tcattgtttg ctcttccacg cccgattatg atgggcagga cagcgatggt
    31921 ccgtgtcatg ggaggctcat atgtcgttct cgctgctggt ttcccaacat caccccaaga
    31981 gccccgaccg cgtcgaggcg tgggaaacag ccttcctcga gctgcaagtc gtcttggcct
    32041 atatcgacag tgttcttgcg ccagccgaac gcatgacgct ttccttgctc aaccttgcga
    32101 agtgggacga aaaggacaat cggcgacaag tcgacgagtt gactcgttcc attgaaaaac
    32161 atgggattga tcaagcggtc cgcagggtca tccgcaagct ggaggagctg tttgagcaag
    32221 aagatcccac gattcgtccg catcttgccc aggaaatatt gcagtctagt gtggcgatcg
    32281 tggtcgcgga cgatgtgatt tcggataatg aggagtcttt tttgcgcgat cttttagcgc
    32341 cggcgcttgg gatggacgag gcgcacgtca tggaggtggt gaaggcggcg tcgggacgac
    32401 tgagccgttt tcgcgcctac gcggagcgtg ggttggaatg ctacttgatg ctcgcagaac
    32461 tccaggaagg tccaccacag ctcaattccc tcgcgggaga caagctgccg ggttttctgg
    32521 gcgccgtgga ccaatgggtg gtgcaacacc ggctcgggtc gagtcgggtg atgggttatt
    32581 ttctgggtgc catgggcggc atcttctgga tcgacagcta cgaacagcac ctttccgaga
    32641 tgacacaact ggtggctgcg gcgcgcacgc tcagccaacg agcaggaccg gaagcacggt
    32701 tacgcgccat ccatcacgaa ctcacgcaac tcagaaatcg cggcgtcgat gccaacggac
    32761 atcgcgctat cgcgcgccat gtcaccaatg ctctttctcg aatggctagc ctcaatgagg
    32821 cgcagagaaa tcgatttcgg gacgcgatcg cgccagcgct cgagatcgat cacgatgcgt
    32881 tggtcgaatc tgcctcccag cgagcctccc tcatcgactt gcacgacaat ctcgtcggcg
    32941 cccattcccc cgagcaagac aagacggaat cgcagtggtg gcgattctgg aagtaagcga
    33001 accccccatg gattcacttg ttttcatcaa gtgaaaccgg tacgccccaa cctcgatgtc
    33061 ccttggattt ttatccgaag gcgagttgtt ctcgctcggt tgtgcgctca tctgggctgt
    33121 tggtgtcatc ctgtttcggg tgagcgggca acgaatcgaa cccattgcct tgaatttatt
    33181 caagaatttg gtggggctcg tgctgttctt catcacgctg ccgctggttg gcgtgtcctt
    33241 ctatcccgaa ggaacgacac ccaccgatct gctgacactc gccatcagcg gcatcgtcgg
    33301 cattggcatc gccgattcgc tgtttttcgc aagcctcaat cgcctcgggg caggcaatac
    33361 cgcaatcgtc gatagcctct acagtccgtt cgtcatcctt tgcgccttcg tctatttgca
    33421 ggaatcgatt tcgagcgcgg tgctcgtagc gatgctcatc atgaccgcgg ctatcttggt
    33481 aggaacgtgg gatccttcgc tgccctccga accacaatcc cgaaaacaaa tcaccgtggg
    33541 cgtggctctt ggcgtactcg gcatgtttct catggccgtc ggactcgtga tcgccaaacc
    33601 cgtactcgag cacgcgcaag catggtgggt gaccaccatg cgcctcgtcg ccggaaccgc
    33661 ttttatcgcc atccaaggac ttctgccgcg gcatcgcgca agtgtgctcg cgattcttcg
    33721 ccccagtcgc cattggcgcg tcgctgtacc cgcaggcatc atcggctgtt atcttgcgat
    33781 gatcacatgg attgctgggt tgaaactcac gttggccagc atcgcaagca tactcaatca
    33841 aaccagcacg ctatttgtcg tcttgctcgc cgccatcttt ttaaaagaaa aagtcacctt
    33901 acgaaaaggc gcggccgtcg ccatggcatt cgtaggagcg gtactcgtca cgtggtagcc
    33961 gaggtaatga ttcggttcgg tgttcgtgcg ggcctagtcc acgctggcgc cgtcttggca
    34021 accgagcgac aacatattgt atttattggt tattttccaa tagctcctca cgcaacgaac
    34081 gggcatcgta taccttgtcg agggcttcca ggatggccga gctatgcacg gaaacagcgc
    34141 ggttcgagcg cgggtcatag gcataatccc cgccaaggga atggatgttg ccatcgaaga
    34201 tcaatccgac gatctgccgc tgcgtatcga acatgggtga accggaattg ccaccgatga
    34261 tgtcgtgggt ggagacgagg ttcatggggg tggagccatt gattttgctt ttagcggtca
    34321 accatgatgc gggaaggtcg aagggaggcg cgccggtatg tcgttcgaaa gcgccggcca
    34381 tggtcgtgaa gggaccgact tgctgtccgt tttcggtcca tccctgcacc acaccaaaag
    34441 ccaggcgcaa agaaaaggtg gcatcggggt acacgctcgt gccgaacacg gcaaatcgcg
    34501 ccgcggcaat tcgttcggcg tttcgtgttt cgacggcttc cacttgttgt tcgaagcgtt
    34561 ctcggatctg gcgagcccac gggtcgatgg ttcgagccag cacgatcgcc gcatcggaag
    34621 aggcttgcac agcggtcttt ccgctttcgt atagccgctt gcgttcgtcg acatgggtga
    34681 gtttggtgcc ggcgattgtt ttttttgcca cttgctcagg ggattcattt cccagggctg
    34741 ctttgacgac aggatggtcg gtaccgagca attcccgtaa ccgagtgagc gacaacgcaa
    34801 gtttcatttc ctcgaattct tggtggatgg gtgcggtgga aaacagctcc tgctggaggg
    34861 cggggagatt cgaatccgca tattcacgaa ggcgttgctc attgggtttg ggcaactcat
    34921 cggacgcgcg cacaatcttt cgcgcatgat ccatcaacgt ggaccaaaac ccctgccctg
    34981 cttccaccaa cgcgtattca ttcgccaatc gttgcttcgc cgcttgcacc gcggcaattc
    35041 gatcccaggc atccccatac ttggcctcga gatccgcatc ggctttgact cgatcccgca
    35101 attggttttc tttctgttgc aactgggaga agaacatcgg atcgagcagc gcttgctgcc
    35161 gcccacgaag cgctttgatc gcgttttccg cgccaaacaa agtcgtgcca gaaacccgcg
    35221 cttgctccac accacgcttc tgaaactcac gcaccgcccc gcgaatctcg agccatcgca
    35281 tcagcaccgc gggcacgcca tgatctcgca tataagcaag ttgagatacg gtcgacatgc
    35341 gtgacgtccg accgggatga ccggatacga aggtcaactg cccctgctcg gcgccatgag
    35401 gagaccagcg aaaatgcgag ggcgtggaag ctggcttgcc gttctcatac acccgaagaa
    35461 acgtcacatc gaggtcgtaa cgggggaagt tgaagttgtc gggatcgcca ccgaaaaacg
    35521 ctatcgacac ttcgggagca aacaccaagc gcacgtcctg ataccgacga tacttgtaca
    35581 aatgatagcg cccaccttga aatagcgtga ccacatcaca tcgaagggca tcgctcgtag
    35641 cacaggattt ctcgatggcc gacatttgct gtttgcgcgc atcattggcc tttgtctcgt
    35701 ccaacccttt cgtcgcttca ttcacacgat cggtgacatc cgtgatctcg atgagttgat
    35761 tgatttccat ctccgggcaa cgcggttcat cggttgcctg tttcgcggtg aaaccgcttt
    35821 ttatgtagtc tttgcgggta gtcgacaact gctggatgca acccacggcg cagtgatgat
    35881 tggtcatgac caagccgcgc gaggagacaa aactaccgga acagcctcgg gccaaacgca
    35941 cggaagagcg catcacttca tcgagccagg cctgggtggg tgcgaagcca tgtcggcgct
    36001 ctaggacttc ggaaggaaag tggttcaaca accacatccc ctcatcggag gaaaacgata
    36061 gcggtggagt tgattcgtcg ggcggagtcg acgatgcgac catcgatgcc gttgtggaac
    36121 ggatggcaga agaggtttgc gcgttgccat ggacgccacc atgacagcca agcccaaggc
    36181 agccacaggc caagcacgtt atcccaaata ttcgcttcac ggcgtcacct cgctccggtg
    36241 gttgaacggc ttcgcaccac cctagtcgtt tcggtgacat cgcaaaaccc cagctaattg
    36301 gtggatttac aagggaaatt ccatagtgcg tcccggctgt cgaaagaggg taggaaggga
    36361 ttgctatgcg acgaggaacg tgctaacccc ctcgcgcgta cactttacga catgtgggat
    36421 attttcatga aacacagtct attttctctg gttatcgccc tgtgtccgct agcggcggga
    36481 tgcgccatgg gcgtgcagga tgaagggttc gaagccgtcg actccaccga gtatgcggcc
    36541 acggcgtgtg gcgccaacta cggagaggcc tttgaatact acaagcgagc ggtcgacgat
    36601 tcgaaggatc gattgcgtta cggggtatgc caaagcgaga acggattcat gtgggcgatt
    36661 gcggataatg cgtcgcgcgc ggtcatgacg tgcggtgcgt ttcgggatgt gattcgcacc
    36721 agcccgtggg cggcgcctct tcgtcaggca cttgctccct ctttgacgct tcggagtctc
    36781 acgggcgagc tgctcgtgat caaggattcc ccgtggcaga attggagtca tgtagaggag
    36841 tttttcgagc agggagtctc attttgggcg cgttctcaag gggcgtacgg ttacagcgtg
    36901 aaggtcgatt ttggcgccaa tggctccgcg gtgtggggtg agcagaccta tgatgagagc
    36961 acgggagaaa tctcctggcg tgtggtttct gccacgtacg cgatcacgaa aaccagttct
    37021 caagcttcgg gtcgtcgtct tgtgaccgtg acgcacgacg gtattgttga gaagttttcg
    37081 cttggggtca tcgatgccga ggcatggaaa gacgcgcctc ttttcgtttt ggatcctctt
    37141 ggcaccggac cggagccggg gcaaggagcc accgtgccgc agctatttag tctcgtgacc
    37201 gagtgcgacg cctgatccca gcgctggttt tcttgcgcgg cggatctaat ttccctaata
    37261 aatatcgata gttaccccat cacggaccga atgtttggcc gtcggacggg ggggagtctt
    37321 tgtggataga tggggagact cgaaggcggg cagcgcgatc aggtcgtggc ttcaggcgtg
    37381 ctatttgggc cggtttcagc cacggcgggt tcggcggcgg gctcgctagt cgcggtcgtt
    37441 tcgccttcgg ttacgaattc gatgagagac atgggggcat tatcgccgcg gcgggggccg
    37501 agtttgatga tgcgggtgta ccccccggcg cgggtcgcga actggggggc cagttcctg
//
LOCUS       DAOSOR010000003        35288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_1243_length_35288_cov_5.018874, whole genome shotgun sequence.
ACCESSION   DAOSOR010000003 DAOSOR010000000
VERSION     DAOSOR010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 35288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35288
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1243_length_35288_cov_5.018874"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1494)
                     /gene="hxsB"
                     /locus_tag="PK710_00320"
     CDS             complement(<1..1494)
                     /gene="hxsB"
                     /locus_tag="PK710_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His-Xaa-Ser system radical SAM maturase HxsB"
                     /protein_id="HOT08174.1"
                     /translation="MSELVPLRTGEATAAGLVPFRVRKIGPDVLLTNPWGDWVFVTPS
                     ELDILASRSLQVGSALYEKLAERGFLRDRLDPAVVAERWNRRKRFLDAGPNLHIIIAT
                     LRCNQTCVYCHASRAQWSDTSTDMSLETAAKVVDFIFQSTSPRLTIEFQGGEPLAHFD
                     LVKYVIERAKEKNRQEDKNLEFTLVSNLSLLDDEKLDYLVNNKVQICTSIDGPAELHD
                     RQRVLASGSSFTEAVRWIERINQRYVQMGLDPVLYHVEALITTTRAALAMPKRIVDTY
                     VDLGCRAIFLRPVDPFGFAAQTGKTIDYDRAAFLDFYRQAVEHILDLNRQGVQVLERY
                     AAIFLTKILGDEDPNFLDIRSPCGAGIGQLAYNYDGKIFTCDEGRMLHQMGDSFFQLG
                     SIDDARYRKIMLTDTVRAMTMASNLDAQPDCVVCAYNPYCGVCPVHNYATQGSIFGRM
                     RESAWCAVHKGIQDYLFDLLRQNDPTTMEILRRWTTLRERDHFIQPTC"
     gene            complement(1494..1727)
                     /locus_tag="PK710_00325"
     CDS             complement(1494..1727)
                     /locus_tag="PK710_00325"
                     /inference="COORDINATES: protein motif:HMM:NF038315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HxsD-like protein"
                     /protein_id="HOT08175.1"
                     /translation="MTELRFRMSLYSSSALDRALEAFASLIDYERESRDEMEVIRLTA
                     RAGEDESWIAGEFANFVLGATVDGVDATRVEEA"
     gene            complement(1724..2281)
                     /gene="hxsD"
                     /locus_tag="PK710_00330"
     CDS             complement(1724..2281)
                     /gene="hxsD"
                     /locus_tag="PK710_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="His-Xaa-Ser system protein HxsD"
                     /protein_id="HOT08176.1"
                     /translation="MSDNHQVHAESTEEGVVLRFEESIYPKDAVFGAAYTLLDRCFVH
                     IDREDSKLLVKLRAKPGITLSADLLAGEFEAEALTQAWRRDIVRENQAWLESVTARAL
                     SGAAGPPGLDDLLEDDLGDLGDAFDDPLGIAVSWEEKYGKGEQADQPMKSSAAPSESS
                     ESSVPQESKPSSDEPEASDEQGEHS"
     gene            complement(2343..3272)
                     /locus_tag="PK710_00335"
     CDS             complement(2343..3272)
                     /locus_tag="PK710_00335"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT08177.1"
                     /translation="MANLGYLQLVRHCNQYCRICSNPETPWVLDLDAAIRALDDFAQR
                     GYAGVILTGGEPSLSEIVEPVVMHATQRGLSVRMITNGSRLADKSLAQRWIEAGLRHF
                     HVSLYSSRREVHDHITCVKGSFDLARHAIRNLGEGGAVLNLNTVINRFNCDHLHELAQ
                     LVVDEMPWVSHVVINNLDPSMGRAETNRQALHRLVDMEVSLHKALGILTAGARTFRVE
                     RVPLCFMVDYAHCSTETRKIIKAEERIIHFLDEKGTVRQTSFRHHKAAVCAHCSLDPI
                     CAGLFEMGHGFDETELAPVFVDPAAIIRRVLRQ"
     gene            complement(3272..4087)
                     /locus_tag="PK710_00340"
     CDS             complement(3272..4087)
                     /locus_tag="PK710_00340"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT08178.1"
                     /translation="MRILQLGPRCNNACIFCANALDRTAPAVATREWMERLAALVPTE
                     ELGVMGGEPTLREDLFDIVRHAKRLGVGRIVLRTNARRFTYVSYAQQLASAGVSGLDV
                     GLYGSSPAMHDYHTDVPGSFLQTLRGIKHATAVGMNVITTCVVTRSNYRHLVDIARLA
                     GSLGAQGVRFRAPEVIGRAAATRSRIVAPPELAMVHLRRVRAQAQRSGLDVSIDRALT
                     VAVDGSSGVQAVDMDRTAKAWPARQEQRSRERRSGDELRDILPSLFDSSEDES"
     gene            complement(4178..6889)
                     /locus_tag="PK710_00345"
     CDS             complement(4178..6889)
                     /locus_tag="PK710_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT08179.1"
                     /translation="MRPGRDEGRGVVHPLAFPRTKVIDFARKKGEPMGDKATTFQLEG
                     YTPEAQSLVAAAQGLADERGHAQVEPLHLLARALDTVPGIDELFRKVGADPAELMNTV
                     ENALKRLPKGTGGVAYLSARLIDLLGRAKRDAEREKAAAVDVEHLLHALSQEIRGAAG
                     DVLASFQLGPGVFRPHISILQSAREAAASRGLKQNDSVQTSLESFTRDLVAIAKLPTA
                     DPVIGLDAQVRRLLQVLERRSKNHPLIVGEPGVGKTALIRALASRIAHGDVPPNLVGL
                     RLLELDTGALVAGARLRGELEERVKSLVSTLTSDQQRHEALLVVENFDAILGQGAVGS
                     GVGELLKPVLARGQLRFLGTTTPEGIRKIQDRDAALLRRLTFITLDPPTVEQAVEILR
                     GITTRYEQHHHVRIGESAIVAAVNLAKRYLQDRELPDSAIDLLDETASRKRVETEGIP
                     PELDDLIRRLESVRAQEAALADDEDEASVATRARLERERAELEPKVRDAKAAVESRRG
                     AFAFVQALRKELEQANTNLEQARGQRDDKRLGELNLVTIPDLKRRLAAAEEAMKATGG
                     EASNRVTDQDVAITLGAWTGIPVAKMLEAETEKLLRMEDRLKMRVVGQDEALLAVSRA
                     VRRGRVGLRDPKRPIGSFLFLGPSGVGKTELAKALAEFLFDDEQALTRLDMSEFMERH
                     MAQRLLGAPPGYVDSEQGGFLTEAVRRRPYSVLLFDEVEKAHGDVFNLLLQLLDDGRL
                     TDGRGRTADFSNTVVIMTSNIGAKRILEAGPTSFETEEGREALRDVLLDELRGFFRPE
                     FLNRIDDVVVFRHLTREHLRGIVDIQLRQLDRLLADRGLRVQLTEAAKMRLVDLGYDP
                     AFGARPVRRALLREVQNPLAEHLLSGTYEAGHVVMVDVRDDVFVFEG"
     gene            6929..7210
                     /locus_tag="PK710_00350"
     CDS             6929..7210
                     /locus_tag="PK710_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HOT08180.1"
                     /translation="MPVKIEIYFATYCGHCHRAMRLLDQKQVPYERIDVTHDPERRAW
                     LRDVTGRRTVPQIFIDGRSIGGSDDLHALDASGQLDEMIGETKLSEGNH"
     gene            complement(7207..7974)
                     /locus_tag="PK710_00355"
     CDS             complement(7207..7974)
                     /locus_tag="PK710_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08181.1"
                     /translation="MAHLSQISILFPLVFALGVAACDEEKKAVDVVKPAASVSAAPTT
                     TVEAPKRMPEVTLHSSAVTVGIDEMALTTPSFDQAFSSMLKKFPVSEPDVLIFNVDRK
                     VKTPVVTKVFYALVDAGAKSIEVRTKPRGSFPDRLVLLSEKVVGDDLPGCTYVGMVLD
                     NLGATFWKKQGGTAKRYTKGMAGPDFSAMHEVMSKEAKSCTSKVFLFSSSAEIDWGHC
                     FDIAGSVKAADPPYEHIDKLVLLREAPVPGKPVKLNP"
     gene            complement(8034..8834)
                     /locus_tag="PK710_00360"
     CDS             complement(8034..8834)
                     /locus_tag="PK710_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08182.1"
                     /translation="MTNRLLASAGILLGTMGWMGCGSTDNPIDKTPKYKYPDVSSFCE
                     AKAKLECTNTVLTRCQTSEDACVIKRRGLCSQSAPTGAKYRPTEAEKCLAAIKGAYAD
                     DQYTAQEKAQEVDACALLWGGFGGEGASCGSNHECDLDKGLRCVKRTGAATGQCYEPE
                     EKQSGQSCEGPDAVCMDGDFCTVSEPNVCANKRAVGSTCSESLPCKDDLRCVGDEAPT
                     CQLKLDVGSPCGSDAECASDMCSPVASTDQCVSVIILAPNEPICANFR"
     gene            9074..12235
                     /locus_tag="PK710_00365"
     CDS             9074..12235
                     /locus_tag="PK710_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT08183.1"
                     /translation="MPNSAGNSSHPQKGVRLGGVRADFVANLGKRLAEMREVWEALSH
                     NPSSTASLDELRRHVHALASRSRVLHFDAVARRLERTQKHLERAAVAGGIDAEDVEEF
                     THLFRDLPELAWNEAGSSQQEPTWEPVDHESERARTDSQPPLSSHSTRVTRASSLPLA
                     PGPASKEEPLAVLVIGRPALADSLRMDSDSSMGSLEVEQTDDLSQALELTRALAPDVL
                     VLDADLLGAVALSESLSLDPLVDSTRVIVVGAFSLPDQTAPFLAHGVMRTLVKPVSPA
                     KLQHAVRSAAKTSTTDDSTDPFGQLTVEELAERLARQLKSELLHTVRPESRRSSVDLG
                     EGMEVQAAVWSAVARIREIVTLRSEGAIRFAQTNPLGAVPFAPWLDALSDGQARRQGS
                     RDNDPEIRFALENRRVLVVDDDPGVTWFLSGLFRAHGSEVREAMDGQQALDTAFVFDP
                     ELVFTDILMPKRDGFGLCRALKRDPLLRDVPVVVLSWKEDLLQRVRDLGVEADGYLRK
                     EATSSSILRTVQECLRPRVRIEQRLKQVGEVRGRLEGIGARTLIKLTAKSLVNASITI
                     RDASYLFEIHLQDGNVVEVTRTAANNESSQGEEALVALLRVFSGRFQIKPRQPHGIPK
                     SSIAFLPIVERVARETRAAQALLEGANLLRVRMVALREDRLDLPATPQSARAALQAIV
                     DGASPRQVIENGIASASTLEQILAHAAMQGAVVSIVDESGTDQFESILRQQAHQQEAE
                     ITVATPTASTMSEQITAPAPVDPKINEPPHPPPTNDSVAPSSLAEAVIRTTGSVAPHE
                     APAPTEPTPMLDAGELKPRASQPFLSPIPSLPPDAVVPGVREQEAQSWDDKDSESDDD
                     SESDDESESDDDSESDDESESDDDSESDDDSDVGEDSRADSAGKRHGASSDTPPHHKR
                     SHLVPIVVGVGIVGGVIGGVLYANSPSMEATDGQSDSATTNSAAAAPDPAVTGTVLEQ
                     LEIDDPSQLSPGQGLLEVVTYEPQAIVYVGEKQGRGEVIAFPLAAGTYPIIVEMGGQR
                     LRREVQVTASKRTRWIVEAEWQR"
     gene            12241..14523
                     /locus_tag="PK710_00370"
     CDS             12241..14523
                     /locus_tag="PK710_00370"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT08184.1"
                     /translation="MALGWWMLAVTGCCLPLSSSESTDSPAHSSSSSPPAPEASGMIV
                     SEDLLASVARCDVYHRGAVIDMGSSLSHGRFDYSFDQPASVQDVQRDGATWGRIKGGV
                     VSLRFEQLEDEPVFVETRIRGLDARAATVRIDGKAVGTLRFAREETQVVSTATSSEPL
                     SAGQHLVSFQFHRSRKDKPVVEMDWIRIGLPDQDPTTYAAPTLRDLATDTMVGERPYR
                     ALALRAPGRVRCAIAATDTMRLQAMLGYAGAGEGEARIEIVEPGKPSVLLHAAKVGGE
                     GHRTEGLDIPLEGLAGRIVALDLVAASTAPGGKVLFGEPMLRIRDPRIADRPDTKVVV
                     LVVFTGANRTHLPGYATVETMPALSSVVEESVVFHQHRAPSTVAAGSMASLLTGYSPA
                     VHKVTDSATRMPDRVSMLSAMGRDRRVATAMFTANPSTFEAFGFNQGWSHYRDFSPVS
                     GASRATPLREAMAWLDNLLKKDKDRQIFLVVHARGGHPPWVGSDDDFKDLPPKEYVGP
                     LTGRRGGQVLAKERNRRYGRAPMTAEDRVRVDAFVRLALRAEDAQLGQLVELLRQHRV
                     WSSTLFMVTSDIAMGGDGRVPFGDGEPMGEDVLEIPLIVRFPGHRFGGSAVHVPTTAM
                     DVARTTLQSLGLEPPEAMEGRDLFEIAAHPGRFAQAPQFSMTGAVYSTRWGNWLLTGV
                     PPRAPKFCEVQPGLECTEDMAARESFFASWMWRLTFDHRQASERVFTPSAIREPATLD
                     PDTVAALTVWGSLEPEPTKR"
     gene            complement(14534..16087)
                     /locus_tag="PK710_00375"
     CDS             complement(14534..16087)
                     /locus_tag="PK710_00375"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOT08185.1"
                     /translation="MSQSSRPELVTLDETNEKALKGDPKRIEKQHEAGKRTARERIDL
                     LLDPDSFVELDRFVTHRCTDFGMQDTKILGDGVVTGWGTVDGRKVFVFAQDFTVFGGS
                     LSGAYAQKICKVMDHAMKVGAPIIGLNDSGGARIQEGVESLGGYADIFLRNTLASGVV
                     PQISAILGPCAGGAVYSPAITDFILMVQKTSYMFITGPDVIRAVTHEEVTKEALGGAA
                     THASKSGVCHLTAPDDAACIALMRELLSYIPSNNMEDPPRLPCSDSVEREIPELDTMV
                     PVESHKPYDIKEIIKAVVDDGRLLEIHEDYAKNIIVGFARVGGRTIGVVANNPMFLAG
                     CLDIHASIKAARFVRFCDCFNIPLVTLVDVPGFLPGTDQEFGGIIVHGAKLLFAFAEA
                     TVPKITIITRKAYGGAYDVMASKHIRADYNIAFPTAEIAVMGPDGAVNIIHRNYLHQA
                     QDPEQERARLIAEYKTAFASPYKAAELGFIDEVIYPRLLRRRIHHALELFKDKRQSNP
                     SRKHGNIPL"
     gene            complement(16160..16867)
                     /locus_tag="PK710_00380"
     CDS             complement(16160..16867)
                     /locus_tag="PK710_00380"
                     /inference="COORDINATES: protein motif:HMM:NF021613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOT08186.1"
                     /translation="MPASFLVIDIETIVDPTLPMPAKAEGSSLPPAPFHQIVAIGALW
                     MSDEYRPIKLGVVGPRKTEPEVLEDFVRFVEERRPDLVTYNGRGFDLPVIVARCLRHG
                     IAFRHYFQPSGMRYRYSAQGHLDLMDYLTDHGASRPVPLDTLTKLIGLPGKVGVDGKD
                     VGPLIHAGMLEQVHAYCLCDVVQTAGLFLRVQLLRGLLDRDQYRAAMEVLLQWMRDDE
                     RLRAVYDGVNQDLLLLR"
     gene            17001..18590
                     /locus_tag="PK710_00385"
     CDS             17001..18590
                     /locus_tag="PK710_00385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOT08187.1"
                     /translation="MPNNLLVVNVEYSETRVALIEEGILTELQTERTRGKTMVGNVYL
                     GKVTRVLPGMQAAFVDIGQDKAAFLHVEDVIRPDDFEQYLAGGDSYATADGSESESDD
                     EAGVDTTVGGSPEKSRSKPARVSRETPIREVLREGQEVIVQVSKDPIGTKGARITSHI
                     SLPGRFVVYTPTIDHIGISRRIGSAVERQRLRDVIETMRPPKGGLIVRTVAEGLLKKQ
                     LKADIGYLVRLWGEIVRKRDTHPKPPCCLYTELDIVLRSARDRLTDDIEQIVIDDPQR
                     CEQLKRFVETFMPERVACIQLYEGNEPIFDAYGIEDEITRALSRKVPLPSGGYLVIDQ
                     AEALTAIDVNTGRFVGKGSKDLEETILKTNLEAVEEIAYQLRFRNLGGLIVLDVIDMD
                     RAANREFVLKRLAELLQKDKAQTTLNPVSELGLIEMTRKRTRESLGRMLHEPCFYCDG
                     TGQLQSRTTIALEILRQIRREHPTLTGYQVTVNAHPAVVDILKNEEREALAATENRLM
                     RRIELVPRREYHIEQFDLQGK"
     gene            18593..18916
                     /locus_tag="PK710_00390"
     CDS             18593..18916
                     /locus_tag="PK710_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOT08188.1"
                     /translation="MADQTTDQDLRRAERITINKEFESFDAFIQEYVSNISRSGVFIR
                     SKTPLPIGTKVNLRFTVIMDDIETIEGTGEVVRIDETGMGVVFRDLTGYSKNLIERLL
                     VQRVD"
     gene            complement(18985..19521)
                     /locus_tag="PK710_00395"
     CDS             complement(18985..19521)
                     /locus_tag="PK710_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08189.1"
                     /translation="MALVDELGECVDYAGVLEPFEIRLTSAHLQIELRHATERLSGAL
                     GGLRELAVYARKRTFFAVHLVDGYDLILVFTAGSPWPLSSRAIAQAEYDIRVEGGWDP
                     PADLERWIHLVVDARPHDRWRPHRVRLAGEWHEVEVMGTVVGLAEGERGFRVRTRTGA
                     EMTLVRERFGHWYADVRY"
     gene            complement(19621..20604)
                     /locus_tag="PK710_00400"
     CDS             complement(19621..20604)
                     /locus_tag="PK710_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08190.1"
                     /translation="MTAVDRGYAPMLGVLLGLLAGCTASESAAHRERVSTLDRGVAAQ
                     LDDQIITMDEVRELSKARNLPVAQARAHLVFDALLATAAHERGLDRTPALLVQRRAVL
                     ARALIDQMKQESKAHAITDEEVAKHTQMHWLDLDRPVARRSTHAVVMPKDPDNEADWK
                     AVDRVAARIADAVRGEKDAEAFKKKAEAVDKEGLEVVIQDLPPVTEDGRVADLTQRLA
                     PGQPPATFDSTFVKILFRLDAVGDQSTPSRSPFGSHVILWVATQEASQLPWERRKEML
                     TEEILATRMREQLEALLSSLRAQSPVSVERNAETVMLLLPDDFVGTDPRGQ"
     gene            complement(20601..20822)
                     /locus_tag="PK710_00405"
     CDS             complement(20601..20822)
                     /locus_tag="PK710_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08191.1"
                     /translation="MGRWISDLAWLDERMGRVLDIVALVLLVMAVGVLVFGIYVLGHR
                     DDLSAIYCFACGAVLLRSSVDLLRPRSAG"
     gene            complement(20880..21398)
                     /locus_tag="PK710_00410"
     CDS             complement(20880..21398)
                     /locus_tag="PK710_00410"
                     /inference="COORDINATES: protein motif:HMM:NF014808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HOT08192.1"
                     /translation="MDFRFVSPDLRQLDSVGSEVLACCLFEDERPVRGLAGLLDWRMA
                     ARLSRWMASEALTGKSGEVVLISGKPRIPFEKLLLFGLGPVSRFDETVYLEIMHRMID
                     TLTGLRVRMAVIERPGRHQDALDALRALDLILEVCEEHGGQDLWTLVEELPDQKRMTQ
                     HLAEERRRKRLR"
     gene            complement(21403..21828)
                     /gene="nusB"
                     /locus_tag="PK710_00415"
     CDS             complement(21403..21828)
                     /gene="nusB"
                     /locus_tag="PK710_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOT08193.1"
                     /translation="MGARTSGRQAALQMLFGLDAVTDADIEAEIRSFWREFEADPEGR
                     TYADSLVRGVMNCRERVDELIRTSSTNWRLERMARVDRNLLRMAAYELLEQTSVPRAV
                     ILDEAIELAKLFGSEDSGAFVNGVLDRIAEEVGRVDLDR"
     gene            complement(21872..22924)
                     /locus_tag="PK710_00420"
     CDS             complement(21872..22924)
                     /locus_tag="PK710_00420"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOT08194.1"
                     /translation="MHPDNHRSEPVLVTGGSGYFGCLLVSHLRRHGHRVRVFDRVDVA
                     DRPADVEFMQGDIRDEHAVRRACQGMAVVHHNVAQVPLAKDRDLFETVNVGGTRVLLE
                     ACRRENVRKVVHVSSSAVFGVPRTNPVDEKQPPHPQEAYGKAKHRAEQVVESYVDRGL
                     DVTIIRPRTILGHGRLGIFQILFELVRRGKPIYVLGRGNNRYQFVHADDLADACILAG
                     QRQGPDLFHIGAERFGTMRALLTALVNHAATGTRLRSLPMRLTTAAMSLTSALGLSPL
                     GPYHALMYGREMFFDGTKAKERLGFHPRHSNEDMICESYDDYLQHREQVLSRAGASHH
                     RSAVRAGVLSLLYYLP"
     gene            complement(22937..23953)
                     /locus_tag="PK710_00425"
     CDS             complement(22937..23953)
                     /locus_tag="PK710_00425"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOT08195.1"
                     /translation="MSLAVVSGAPGWLGTSLTEALVQGARFRSISTEPRRVRCIVQPQ
                     ADPAPLLALGDKVEIVRADLRDRRSLVGLCKDADTVFHCAGIIHPHRIQDLYDINVRG
                     TQNLLDQAVEAGVRRFLFVSSNSPAGLNATPSILMVEQDPPRPYLNYGLSKLQSEWLI
                     NDAHRSGKIETVILRPCWFYGPNQPERQSRFFQMIKGGHPIIVGKGDNLRSMSYIDNI
                     VQALLLAEKEQKAGGKTYWIADRRPYSFIEIIQTVARVLDVAVRPRYLPRVTSDMARF
                     VDSLMQMSGLYNQEIHVAGELAATIAVSIEAAQRDLGYDPEIELEEGMIRSIAWCRAH
                     GQPL"
     gene            24128..26077
                     /locus_tag="PK710_00430"
     CDS             24128..26077
                     /locus_tag="PK710_00430"
                     /inference="COORDINATES: protein motif:HMM:NF014466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOT08196.1"
                     /translation="MGSFRHWIVGSALFTLGSLPILAKAQTPSLDVRHFQPPTSPDGS
                     LYVETTNSPGASQWNVGSWLSWSFRPVVLRDGNDDVVANLISQQLSLDLLGSIGVTRH
                     AAIGLAVPAVLAQTGDSNEYTNTVLHGSTLSSQALGDIALTGKVTLMAIEPMGGWGFA
                     ALARVTAPTGSRTAYVGEGALTSELRFLAEYDLLAASVLATTGFKLRTQERTFAGVTY
                     GNEIPWGIGVKVLPQAIGLDDSGRWTWSLEAHGALPAGPANPFSSVALSPALLGASAR
                     YRIGTDLTLAGGVEAPLDGAVGVPLTRVLVGISYAPRNHDMDGDGIPDDIDQCPELAE
                     DFDGFEDDDGCPDYDNDADGVPDAMDQCPNEPEDLDDFEDDDGCPDPDNDRDGILDEN
                     DACPDVWGLPSQDPSKHGCPVLDRDSDGIPDDLDQCPDEPEDKDGFQDEDGCPDLDND
                     GDGIPDAEDQCPLIPGPASPATKWHGCPVPDQDGDTFDDEHDKCPQEPETWNGVEDDD
                     GCPDQGGLWLAREQTTREGPSLVIRTPIRFVGPTHAPSIHADSVPTVRAIATILNRHP
                     DWVLAVGTKPNPIDGPLDSTHSLSRAFAIVLALQSYTFRDGVAETVSWEAVRTQPGAI
                     AQGVGFLVLYGAAETTASDQEEAAP"
     gene            26074..28113
                     /locus_tag="PK710_00435"
     CDS             26074..28113
                     /locus_tag="PK710_00435"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT08197.1"
                     /translation="MKRNPISAWCVGAALLMAAAPTTARAQASDDTQESVASRSWTTR
                     FGPNVARRWLRSGRFEDRLRAVRRIGSQNDYTSVQILVQALTDDPALASSPQIRLESV
                     RALAPFVSRDPVRILLTGWMTPPNNQLANPLINLAADQASMALAASGNTRAIEQLVMA
                     LAENDQPRAVQALRAHPPANLEPLLQARFRESTQVIDLIGHLGDLRAIPALRKLLHNA
                     DLNVQVAAAIALARLGDASGCGKAKDWIRQEGSTHALRVGATQTLVLCRDPWAPRAMA
                     ILLADPATRQQGLDLVEIAPTAQCSPTLAGLLTIAEDRERVRVIAALARCGGPLAART
                     LESLLRDGNTDAAFALAQCRGPEASQALSRALADPKTKRLAAWAGVLRYTEMAIIAEG
                     LGDALTSLASSHAPADRFIGVFGLVLLGDVQRIEAAQDPVAVVAACNAASTLSADKRE
                     PCIEKLKGTRDPAMRDGLAGCLHDIDSIDGLSTATLFEWAESERISAPVFGRLLGVRD
                     SESSRKRIERLLASSDPDMRAQVALGLAHSPQPSALSLLGEALTWETEPMVRRAIVTA
                     LQRRQSTLGQKWLNQAAHLDPDPIVRSLARPHPFARPITQQQQWFWLRLGESQATQTA
                     SHAVRVMLPHGSASVIITAEDGMALVPGVTYAETRLRVDGVPWQFTSSRTQEGQR"
     gene            28110..28358
                     /gene="xseB"
                     /locus_tag="PK710_00440"
     CDS             28110..28358
                     /gene="xseB"
                     /locus_tag="PK710_00440"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOT08198.1"
                     /translation="MSPKKQPTDENATFEQSLQQLAEIVDKLEGGDLPLDESVALFEK
                     GMKLAKHSQALLQRAEKKVEELLAVEPDGSPITRPYEE"
     gene            28486..29349
                     /locus_tag="PK710_00445"
     CDS             28486..29349
                     /locus_tag="PK710_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOT08199.1"
                     /translation="MTNQDIASKGEAETVQTEETAASQDRIPSNIPSKEIADAPSDNE
                     PSPSESEASGEKAGPHEATAETVEESDEEAIAIEEEVIEDPLQVARDENKALREQMLR
                     LAADFDNFRKRSRRDIEDAARGAKMDVLRDLLPVFDNLERAETHAKQATDVQAVATGV
                     AMVIRQFEDIIGKFGVERVKSVGQPFDPVIHEAIQMLETKEQSPGTVMAEVLGGYRWG
                     DRLLRAAMVVVSKAPAQERASGEHETASDTEAAGEQAEQAESEQDSEKSESTGESAVF
                     EQNDEDPTAES"
     gene            29412..31235
                     /gene="dnaK"
                     /locus_tag="PK710_00450"
     CDS             29412..31235
                     /gene="dnaK"
                     /locus_tag="PK710_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOT08200.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEDGAADAKPVVIPNSEGARTTPSVV
                     AFTTSGERLVGHVAKRQGITNPEHTVFAVKRLMGRKFSDREVRRQIELAPYRVIEASN
                     GDAWIRGRDREYSPPEISSIVLDKMREVAETYLGEKLTEVVVTVPAYFDDAQRQATKD
                     AGRIAGLEVKRIINEPTAAALAYGLSLKERETIAVYDLGGGTFDISVLQIEGGVFGVR
                     ATGGDTHLGGEDFDQRIIDTLAEEFEMENQVDLRKDRMALQRLKEAAEKAKHELSSSL
                     ETEINIPFIATGPTGPLHLQRTMKRSDLEILTSDLIDRTLDACRAALQDAKLSARDID
                     NVVLVGGMTRMPAVQKSVKEFFGKEPCRSVNPDEVVAVGAAIQAAALAGEVDEVLLLD
                     VAPLSIGVETGGGVFFRMIERNRTIPTEASGVFTTSVDNQSFVPVHVLQGEREMAADN
                     RSLAHFELTGIPPAPRGVPKIQVTFRVDANGILHVEARDLGTNRSQAIKVTPTSGLSQ
                     DEIDRLVQEGDKFRETDSLRKELAEMRNQAETLLYTTEQALDGYADLLDEQVVAEVRG
                     QAETLRGLLKSSGSFEDIRSAYQELESAAFRIAESMYGSES"
     gene            31398..31934
                     /locus_tag="PK710_00455"
     CDS             31398..31934
                     /locus_tag="PK710_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HOT08201.1"
                     /translation="MSFRLIAFESAGVLAEAWMVNPQMVMYEEEFNQIQTVVDRLVRD
                     ANAKVVFIVDKNGQLIAASGDVENLDTTSLASLTAGNIAATGGMAKLLKENEFATQFH
                     EGEKANIHIQLVGNRVILVVIFDARSSLGLVRLRVKKASDELNRIFEALLRKVQEPGG
                     ESPFAEITDEDIDNLFND"
     gene            31944..32531
                     /locus_tag="PK710_00460"
     CDS             31944..32531
                     /locus_tag="PK710_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HOT08202.1"
                     /translation="MSFINYMAREINCKIVYYGPGLCGKTTNLQYIYDRTNPDAKGKM
                     ISLATETERTLFFDFLPLSLGEIRGFKTRFHLYTVPGQVFYDASRKLILKGVDGVVFV
                     ADSQIMRMEANMESVDNLRVNLAEQGYALEKLPYVVQYNKRDLPAVADVDELREMLNP
                     DGVPDFEAVARTGVGVFETLKAVAKLVLTELKRGG"
     gene            32824..33579
                     /locus_tag="PK710_00465"
     CDS             32824..33579
                     /locus_tag="PK710_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08203.1"
                     /translation="MNGAWKRGWVLSVMMGGIITSIASCSENESSIFIRQVLKPSGAD
                     CSFSAEPGGSALSTGVMDLAFTREYQAGLLLGNQLVERGSADQLRTETARFLAEGAEV
                     ELETTDGKGINSFTVPVSGFVDPSTGTEPGWGVVTATLIDSGTGQGLAAGFPEGQRST
                     VEGLVVAVVKVFGHTLGGKELDSGKYRFPISICYGCLVDFPPESVDPDLPLPNCANVS
                     KSTGTVPCMIGQDEPVDCRLCQAQGAPASLCNP"
     gene            33674..34240
                     /locus_tag="PK710_00470"
     CDS             33674..34240
                     /locus_tag="PK710_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08204.1"
                     /translation="MRPCKLLALLIIATLFFGSFASFASYAHAQPQSNLAVTLGAALT
                     QNPSATRFHTALHGDILFGRTSPWDFGIGPYADLATAWNDFSVGTGASLLLPIHDALP
                     IVLSAGPFVRAFDSRESIGLSTDLFWGSRSFNYHGSYAMAFGLILQGRTAFGAEPDRA
                     LILGFRIDGQAFALPLLLAYGAITGGEP"
     gene            34237..34635
                     /gene="zapA"
                     /locus_tag="PK710_00475"
     CDS             34237..34635
                     /gene="zapA"
                     /locus_tag="PK710_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOT08205.1"
                     /translation="MSDRPKRLTSIRLAGRHLRVRTNASDHHLARIVGMIEARANAIA
                     AGKPLTPDTFLLVAMSFAEEAAEARARADRILSMAQQTAQTLLNRVESALDLPSEHDE
                     SADLQAESMVDDDLESCEHQLITPDPNDAT"
     gene            complement(34709..>35288)
                     /gene="ccmA"
                     /locus_tag="PK710_00480"
     CDS             complement(34709..>35288)
                     /gene="ccmA"
                     /locus_tag="PK710_00480"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HOT08206.1"
                     /translation="GGTAVLRGLSGRMQKGVLHLLVGPNGSGKSTLLRIVAGLLSPSF
                     GQVEYEGVARSRIREAIGLVSHELLTYGELTVEENLRLGSGLYAAGEQDVEQAMNRFG
                     LGAVRDRMVSKLSRGQRQRVSLARALLHRPSLVLLDEPTTGLDEQGVAAIVRVVRQEV
                     ASERVVIVVTHDPSYFDIRERQIWRLEKGRWA"
BASE COUNT         7745 a        10068 c        10114 g         7361 t
ORIGIN      
        1 acaggttggt tgaataaagt gatcgcgttc acgaagggta gtccagcgac gaaggatttc
       61 catggtagtc ggatcgtttt gtcgcaacag gtcaaatagg taatcctgta tccctttatg
      121 gacagcgcac caagcgcttt ctcgcattcg tccaaaaatg ctcccttggg ttgcatagtt
      181 gtgaacgggg cacactccgc aataaggatt gtaagcgcac accacacaat cgggttgggc
      241 atcgaggttg gatgccatcg tcatggctcg caccgtatcg gtgagcatga tcttgcgata
      301 ccgggcgtca tcgatggatc cgagttggaa gaacgaatcg cccatctgat gaagcatgcg
      361 cccctcgtcg caggtgaaga tcttcccatc atagttgtag gcgagttgtc cgatccctgc
      421 gccgcaaggc gatcggatat cgaggaagtt gggatcttca tcacccaata tcttggtgag
      481 aaagatggcc gcgtaccgct cgagaacctg gacaccctga cggttgaggt cgaggatgtg
      541 ctccaccgct tgtcgataaa aatcgagaaa cgccgcgcgg tcatagtcaa tggttttccc
      601 tgtctgtgcg gcaaaaccaa aaggatcgac cggacgtaaa aaaatcgctc ggcatccgag
      661 gtccacgtac gtatccacga tccgtttggg catcgccaag gcggcccgtg tggtcgtaat
      721 caatgcttcc acatgataaa gaacgggatc gagccccatc tggacatagc gttggttgat
      781 tcgctcgatc catcggaccg cttccgtgaa ggaacttccc gacgcaagca cccgctgacg
      841 gtcatgtagt tccgcgggtc catcaatgct cgtgcatatc tgaaccttgt tattcaccag
      901 gtagtcgagt ttttcgtcat cgagcaggga gaggttcgaa accaaggtaa attctaggtt
      961 tttatcttcc tggcgattct tttccttggc ccgttcgatg acatacttca caaggtcgaa
     1021 gtgggcgagg ggctcgcctc cctgaaactc gatggtcaaa cgaggcgagg tgctctgaaa
     1081 gatgaaatcg accaccttgg ccgccgtttc caacgacata tccgtcgatg tatccgacca
     1141 ttgtgcgcga cttgcgtggc aatacacaca ggtctgattg catcgcaacg tcgcgatgat
     1201 gatgtgcaag ttggggccag cgtcgagaaa tcgttttcga cgattccatc gttcggcgac
     1261 caccgccgga tccagtcgat cccgcaggaa cccccgctcc gcaagttttt cgtataacgc
     1321 ggaaccgacc tgcaacgatc gagaagcgag aatgtccaat tccgacggtg tcacgaaaac
     1381 ccaatccccc caaggattgg tgagcaggac gtcaggaccg attttgcgaa ctcgaaacgg
     1441 caccaaacca gccgccgtcg cttcccctgt ccgtaacgga acgagttccg acatcacgct
     1501 tcctccacac gtgtcgcgtc cacgccatcc acggtcgccc caagcacgaa attggcaaac
     1561 tcgccggcga tccacgactc gtcctcacca gctcgggcgg tcaaccgaat gacttccatc
     1621 tcatcgcggc tctcgcgctc gtagtcgata agagaggcga aggcctcgag ggcccgatcg
     1681 agcgcggagg atgaatacaa actcattctg aagcgaagtt ctgtcatgag tgttcccctt
     1741 gctcatcgga agcttcgggt tcgtcagagg aaggcttcga ctcttggggt accgacgatt
     1801 ccgagctttc ggacggagcc gcggaggatt tcatgggctg gtcggcttgc tcgcctttgc
     1861 cgtacttctc ttcccagctc acagcgattc ccaagggatc atcaaaagca tctcccaaat
     1921 cgccgaggtc gtcctcgagc aagtcatcga gacctggagg cccggccgcg cccgaaaggg
     1981 cacgcgccgt cacgctctcg agccacgcct ggttctcccg aacgatatct cttcgccagg
     2041 cttgggtgag agcctcagcc tcgaattcgc ccgccagcaa gtccgcggac aacgtgatcc
     2101 ccggtttcgc tcgaagcttc acgagcagct tcgagtcttc ccgatcgatg tgaacgaaac
     2161 atcgatcgag aagcgtgtag gccgcaccga atacggcatc cttcggatag atcgactctt
     2221 cgaaacgcag taccacaccc tcttcggtcg attctgcatg cacctgatgg ttgtcgctca
     2281 tgatgcatcg gtttagtgaa gtgccgacga gcggacaagc ctctcggtgc tggatggtaa
     2341 attcactgtc gtagaacgcg acgaatgatg gcggccggat cgacgaacac cggggccaac
     2401 tccgtttcat cgaagccatg ccccatttcg aataatccgg cgcaaatggg atccaacgag
     2461 caatgggcac agaccgccgc cttatgatgg cggaaactcg tctgacgaac cgttcctttt
     2521 tcatccaaaa agtggatgat tcgctcttcc gctttgatga tctttcgcgt ttcggtggag
     2581 caatgagcgt aatcgaccat gaaacacagg ggcacacgtt ctacccggaa ggttctggct
     2641 cccgcggtca gaatcccgag cgccttgtgt agagatactt ccatgtccac gagacgatga
     2701 agagcctgcc gattggtttc cgcccttccc atcgacgggt ccagattgtt gatcacgaca
     2761 tggctcaccc aaggcatttc gtcgaccacc aattgggcaa gctcgtgcaa gtgatcacag
     2821 ttgaatcgat tgatgacggt attgaggttc aacaccgcac ccccctcacc aaggttgcga
     2881 atggcgtggc gagcgaggtc aaaagaaccc ttcacacacg tgatatgatc gtgaacctca
     2941 cgacgactcg aatacaacga aacgtgaaaa tgccgtaatc cggcctcgat ccaacgctga
     3001 gccaaggatt tgtccgccaa tcgacttcca ttggtgatca tgcggacaga caatcccctt
     3061 tgcgtggcat gcatgaccac gggctcgacg atctccgaaa gcgaaggctc ccccccggtg
     3121 agaatgacac ccgcgtatcc gcgttgggcg aagtcgtcga gcgcacggat cgccgcatcc
     3181 aagtccaaga cccacggcgt ttccggattg gaacaaatgc ggcaatactg attgcagtga
     3241 cgaacgagct gcaaataacc gaggtttgcc actaggactc atcctcgctg gagtcaaaga
     3301 gggagggaag gatatctcgc aactcgtcac cggaacggcg ctcacgactt cgttgctctt
     3361 gacgagccgg ccaggctttt gcggtcctat ccatgtccac ggcttgcaca cccgacgaac
     3421 catcgacggc aacggtcaag gctcgatcga tcgatacgtc gagccccgaa cgttgtgctt
     3481 gcgctcgcac acgccgcaaa tggaccatcg cgagttcagg gggggcaacg atacggctgc
     3541 gcgtggcggc ggcacgtcca atcacctcgg gtgctcgaaa tcggacccct tgcgcaccca
     3601 gcgaaccggc taaccgcgcg atatcgacca gatgtcgata attcgaacgg gtgaccacac
     3661 acgtggtgat cacgttcatt cccacggcgg tggcgtgttt gattcctcga agggtctgca
     3721 aaaatgaacc gggcacatcg gtgtgatagt cgtgcatcgc gggcgaagaa ccgtaaaggc
     3781 ccacgtcgag ccccgaaact ccagcggaag caagctgctg cgcatacgag acatacgtaa
     3841 accgtcttgc gttggtccga agcacaatgc gacccacacc gagtcgtttg gcgtgtcgaa
     3901 cgatatcgaa caagtcctca cgaagggttg gctctccacc catcacgcca agttcttcgg
     3961 tgggcacaag agccgcgagt cgctccatcc actcgcgggt cgctaccgcg ggtgcggtac
     4021 gatccaatgc attcgcgcag aatatgcacg cgttattgca ccgcgggccg agttggagaa
     4081 tccgcatgga ccgtacgtat attcatactc gcaatgggaa agaacaccag ggagcgcgag
     4141 cgaattgctc gctcaccatc gacgcaacgg gcacggttca tccttcaaac acaaatacgt
     4201 catcacgcac gtcgaccatc accacatgcc cggcttcata ggtgccagag agcaagtgtt
     4261 cagccagcgg attctgaact tcccgcaaca acgctcgtcg cacaggacga gcgccaaagg
     4321 caggatcgta tcccaagtcg accagacgca ttttcgccgc ctcggtcaac tgcacgcgta
     4381 gcccccgatc ggcgagcaac cgatcgagtt gtcgaagctg tatgtctacg atccctcgaa
     4441 gatgctcccg cgtcagatgc cgaaacacca cgacatcatc gatgcggttg agaaattcgg
     4501 gacggaaaaa cccgcgcaac tcatcgagca gcacatcgcg caaggcctct cgcccctctt
     4561 cggtttcaaa cgatgtcggc cctgcctcca agatccgctt tgcaccgata ttggatgtca
     4621 tgatcacgac cgtattcgaa aagtccgcgg tgcgaccccg tccatcggtc aaccgaccat
     4681 cgtcgaggag ctgcagcaat aggttgaaca catcaccatg agccttttcg acttcatcga
     4741 aaagaagcac ggaataagga cgacgtcgca cggcctcggt gagaaaacct ccttgctcac
     4801 tgtcgacata tcccggagga gcgccaagca gacgttgggc catgtgacgt tccatgaact
     4861 cgctcatgtc gagacgggtc agcgcttgct cgtcatcgaa cagaaattcg gccaaagcct
     4921 tggcgagttc ggtttttccg acgccactgg gtccaagaaa aagaaaggaa ccgatcgggc
     4981 gttttggatc gcgcagtcca acccgtcctc gtcgcacggc gcgactcacg gccaacaacg
     5041 cttcatcctg ccccacgaca cgcatcttca gacgatcttc catccgaagg agtttttcgg
     5101 tttccgcttc gagcattttt gccacgggaa tgccggtcca agcgccgagg gtgatggcca
     5161 catcttgatc ggtgacacgg ttggatgctt caccaccggt ggctttcata gcctcttcgg
     5221 ccgcggccaa tcggcgcttc aaatcgggaa tggtcacgag attcaattcg ccaagccgtt
     5281 tatcgtctct ttggccacgc gcttgctcga ggttggtatt ggcttgctcc aattcttttc
     5341 gtaacgcctg cacgaaggcg aaggcccctc gcctggattc gacggccgcc ttggcatcac
     5401 gcactttcgg ttccaattcc gcacgctcgc gctcgagacg tgcgcgagtc gcaacgctcg
     5461 cctcatcctc atcgtcggca agcgccgcct cttgcgcacg aaccgactcc aaacgtcgaa
     5521 tcaaatcatc caattcagga ggaatgccct ccgtctcgac acgtttgcgc gatgccgtct
     5581 catcgagaag gtcaatggcg ctatcgggca actcacggtc ttgcaagtaa cgcttcgcaa
     5641 ggttcacggc agcgacaatc gcggattctc caatccgtac gtgatggtgt tgctcgtagc
     5701 gtgtggtgat tccccgtaaa atctctacgg cttgttcgac cgtgggaggg tcgagggtga
     5761 tgaaggtcaa gcgacgcaac aaggctgcgt cgcgatcctg aatctttcgg atgccttctg
     5821 gcgtagtcgt accaagaaag cgaagctgac cgcgagcgag cacgggtttg agaagctcgc
     5881 ctacccccga gcccacggcg ccctggccga gaatggcgtc gaagttctcc acaacgagga
     5941 gggcttcgtg gcgttgttga tccgaggtca aggtcgaaac gagagatttg acgcgttctt
     6001 ccaactcacc acgaaggcga gcccctgcta ccaaggcacc cgtatccaat tcgagcaacc
     6061 gcagtcccac caggttcgga ggaacgtccc catgggcgat tctagacgca agtgcgcgaa
     6121 ttaatgctgt ttttccgact cccggttctc ccacgatgag cggatggttt ttgcttcttc
     6181 gctcgaggac ttgcagcaat cgtcgcacct gagcatcgag gccaatcacc ggatcggccg
     6241 tgggtagttt ggcgatggcg acgaggtcac gggtgaagga ttcaagggag gtctgaaccg
     6301 aatcattttg tttcaagccg cgggaagcag cggcttcgcg tgcggattgg agaatggaaa
     6361 tatgcgggcg aaatacgccg ggaccgagtt ggaaagaggc gagcacgtcg cccgcggcac
     6421 cgcgaatttc ctgggaaagc gcgtgcagca ggtgctccac gtcgaccgcg gcggccttct
     6481 cccgttccgc atcgcgcttg gcgcgtccca acaaatcgat gagacgcgcc gataggtagg
     6541 ccacaccccc tgttcctttc ggcaatcgtt tgagagcgtt ttccacggtg ttcatcagtt
     6601 ccgcagggtc ggcgcccacc ttgcgaaaca gctcatcgat gcccggaacg gtatccaagg
     6661 cgcgagccaa caagtgcaag ggctctacct gcgcatgccc acgttcatcc gccaaccctt
     6721 gcgccgccgc cacgaggctt tgcgcttcgg gcgtataccc ctcgagctgg aacgtcgttg
     6781 ccttgtctcc catcggttca cctttctttc gcgcaaaatc tatcaccttc gtccgcggaa
     6841 aggccagcgg atgcaccaca ccccgccctt catcccgacc tggacgcatg tcatcatggc
     6901 gccatcgtcc ctttcggaag gttaggccat gcccgtcaag atcgaaatct acttcgcgac
     6961 ctactgcggc cactgtcatc gggccatgcg cttgctggac caaaaacaag ttccctacga
     7021 acgcatcgat gtcacccacg atcccgaacg ccgggcgtgg ttaagggatg tcacaggacg
     7081 tcgaacggtc cctcagattt ttatcgatgg tcgaagtatt ggaggatccg acgacctaca
     7141 cgcgctcgac gcatcgggcc aattggacga gatgataggg gaaacgaagt tatccgaggg
     7201 aaaccattag gggttgagtt tgacgggctt gccgggaacg ggagcttccc gaagcaacac
     7261 gagtttgtcg atgtgttcgt aaggcggatc ggcagctttc accgagcccg cgatatcgaa
     7321 gcaatgaccc caatcgattt cggcagagga cgagaaaaga aatactttgg aggtacatga
     7381 tttggcttct ttgctcatga cctcgtgcat ggcgctgaaa tcgggacccg ccattccttt
     7441 ggtgtaccgc ttcgcagtcc caccctgttt tttccaaaac gtggcgccta gattatcgag
     7501 caccatcccc acgtaggtac agccaggcag atcgtcgcca accacctttt cggataacaa
     7561 cacgagacgg tccgggaatg atccccgagg cttggtcctc acctcgatgc ttttcgcacc
     7621 ggcatcgacc aacgcataaa acaccttcgt aaccaccggt gtcttgacct tccgatcgac
     7681 attgaaaatc aatacgtccg gttcgcttac cggaaacttc ttgagcatgc tcgaaaacgc
     7741 ctgatcgaac gaaggcgtgg tcagagccat ttcgtcgatt cccaccgtga ccgcggaaga
     7801 atgcagggtc acctcgggca tacgcttggg ggcttccacg gtcgtcgtag gtgccgctga
     7861 cacggaagcg gcgggcttga ccacgtccac ggcctttttc tcttcgtcac aagccgcaac
     7921 gccaagagca aagacaaggg gaaagaggat cgatatttgg gaaaggtgcg ccatgggcgg
     7981 gcagtgtact ccagcgggaa cacaccgtca tccgaacccg tcaatcgcga taattaccgg
     8041 aaattcgcgc agatcggctc gttgggcgcg agaatgatca cgctcacaca ctgatccgtc
     8101 gatgccacgg gagaacacat atcgctcgcg cattcggcat cgctcccaca aggggaacca
     8161 acatcgagct tgagctggca cgtcggtgct tcgtctccta cgcaccgaag atcgtctttg
     8221 caaggcaacg attcgctaca ggtactgccc actgctcgct tgttggcaca tacattgggc
     8281 tcactgacgg tgcaaaaatc cccgtccatg cataccgcat ccggtccctc acacgactgg
     8341 cccgattgct tttcctccgg ttcataacac tgcccggtcg ctgctcccgt tcgcttcaca
     8401 caacgcaacc ctttgtccaa atcgcattcg tgattcgaac cacacgacgc cccttcacca
     8461 ccgaatcctc cccaaagcaa agcacaagca tcgacctcct gagccttctc ttgcgccgtg
     8521 tattgatcgt cggcatacgc gcctttgatg gcggcgaggc acttctccgc ttcggtcggc
     8581 ctatacttgg cccccgtggg agcgctttga ctgcacagcc cgcgacgttt gatcacgcag
     8641 gcgtcctcgg aggtctgaca tcgcgtcaac accgtgttgg tacactccaa cttcgccttg
     8701 gcttcgcaaa acgacgacac gtctggatat ttgtattttg gcgttttgtc gatcgggttg
     8761 tccgtacttc cacaccccat ccatcccatg gttcccaaca ggattccggc tgatgccaac
     8821 aagcggttcg tcattgtcta agcctccacg acacggccaa gcctcaaccg gcaattggcg
     8881 caatcgaatc attgtccagc tcgttgtatt acgccagaac tgccccgcgg atccctcatg
     8941 gaaggatatc ttgttccgtc tcagttataa catattgatt ttattgatga ataatattga
     9001 ccggccaagg gattgcggag aaggaccgac gggctagttg ggagggaagg ctcgatatgg
     9061 tacggggcca aggatgccca acagcgcagg caatagctcc cacccccaaa aaggcgttcg
     9121 tcttggaggt gtgcgcgcgg atttcgtcgc caatctagga aagcgactgg ccgaaatgcg
     9181 cgaggtatgg gaggcccttt ctcacaatcc cagttcaacc gcctcgttgg acgagcttcg
     9241 gcgtcacgtc catgcgctcg caagccgttc gagggtattg cacttcgatg cggtagcccg
     9301 acgcttggag cgcacgcaaa agcatttaga gcgggcggcg gtcgcgggag gcatcgacgc
     9361 ggaggacgtc gaggagttca cccatttgtt tcgggacctt cccgaactcg cctggaatga
     9421 agcaggaagt tctcagcaag agcccacgtg ggaacctgtc gatcatgaat ccgagcgggc
     9481 tcgaaccgac agccagccac cgctttcctc gcattccacg cgtgtcacac gcgcgtcttc
     9541 tttgccgctt gctcccggcc ccgcatccaa agaagaaccg cttgccgtac tggtcattgg
     9601 gagacctgcc cttgccgact cgcttcgcat ggacagcgat tcatccatgg gctcgttgga
     9661 agtggaacag acggatgacc tgtcccaagc gctcgagttg acacgagccc tcgcgccaga
     9721 tgtgctcgtg ctcgatgccg atctgcttgg cgccgtcgct ttgagcgaat cactttcgct
     9781 cgacccgctt gtcgattcga cccgcgtcat cgtcgtgggc gccttttctt tgcctgatca
     9841 aaccgcaccg tttctcgccc atggcgtgat gcgaaccttg gtcaaaccag tgtcacccgc
     9901 caagcttcaa cacgcggtgc ggtcggcggc aaaaacgtcg accacggatg attcgaccga
     9961 tccctttgga cagcttaccg tggaagaact ggccgaacgt cttgcacgac agctcaaatc
    10021 cgaattgctg catacggttc gacctgagtc tcgacgatcg tcggtggact tgggcgaagg
    10081 aatggaagtg caagcggccg tatggagtgc ggtggcgcga atccgtgaga ttgtcacgct
    10141 ccggagcgaa ggagcgatcc gtttcgcgca aacgaatccc ctgggggccg tccctttcgc
    10201 gccttggctc gatgcgttgt ctgatggcca ggcccgacgg caaggttcgc gagacaacga
    10261 tcccgaaatc cgattcgccc tcgagaatcg tcgcgttctc gtggtggacg acgacccggg
    10321 ggtgacttgg ttcctcagtg ggttgttccg tgctcatgga agcgaagtgc gagaagccat
    10381 ggacggccaa caggctctcg ataccgcatt cgtcttcgat cccgaactgg tgttcaccga
    10441 cattctcatg cctaaacggg atggtttcgg actgtgtcgg gcactcaaac gagacccgct
    10501 gctgcgagat gtacccgtcg tcgttttgtc atggaaagag gacctgctcc aacgtgtacg
    10561 ggaccttggg gtcgaagcag atggatactt gagaaaagag gcaacttctt cgtcgatcct
    10621 tcggaccgtt caagaatgcc tgcggccccg tgttcggatc gagcagcgtc tcaaacaagt
    10681 aggggaagtg agagggcgtc tcgaaggaat tggcgcgcgc actctgatca aattgactgc
    10741 aaaatccctt gttaatgcgt cgattaccat tcgtgatgcc agttatcttt tcgagattca
    10801 tcttcaggac ggcaatgtgg tcgaggtcac acgaacggca gcgaacaacg agtccagcca
    10861 gggggaagaa gcgttggtcg ccctgcttcg tgttttctct ggaagattcc agatcaagcc
    10921 gagacaaccc cacggcatac caaaatcatc cattgcgttt ctcccgatcg tcgaacgcgt
    10981 agcgagggaa acgcgcgcgg cgcaagcttt gctggaaggg gcgaacttgc ttcgcgttcg
    11041 gatggttgcc cttcgcgagg accgacttga tttgcccgca acaccccaat ccgctcgagc
    11101 agcattgcag gcgatcgtcg atggtgcatc cccgcgacaa gtcatcgaaa acgggatcgc
    11161 ttcggcttct acgctcgaac aaatcctcgc ccacgcggcc atgcaaggcg ctgtcgtctc
    11221 catcgtggat gaatccggca ccgatcagtt cgagtctatc ctacggcagc aggcccatca
    11281 gcaagaggcc gagatcacgg tcgctacgcc gactgcctcg acaatgtcag agcagatcac
    11341 ggcacccgca cctgtcgatc ccaagatcaa cgagcctccg catcccccac ctaccaacga
    11401 ttcggtggcc ccttcatctc ttgccgaagc tgtgattcga accacgggga gtgtagcgcc
    11461 ccatgaggcg cccgcgccca ctgagccaac accgatgctc gatgctggtg aactcaaacc
    11521 tcgcgcgagc caacctttcc tttcaccaat tccttctttg ccacccgacg cggtcgttcc
    11581 tggagttcga gaacaagaag cccaatcgtg ggatgacaag gattcggaat ccgatgatga
    11641 ttcggagtcc gatgacgagt cggaatccga tgacgattcg gagtccgatg acgagtcgga
    11701 atccgatgac gattcggagt ccgatgacga ttccgacgtt ggggaagact ctcgcgccga
    11761 ttcggccgga aaacggcacg gagcctcatc ggacactccg ccacaccata agcgatctca
    11821 cctcgtgcct atcgtcgtgg gagtcggaat cgtggggggt gtgatagggg gtgtgctgta
    11881 cgcgaattcg ccatcgatgg aagcgacgga tggacagagc gattcagcaa caacgaattc
    11941 agccgcggca gcacctgatc cggctgtgac aggtacggtt ctggagcaac tcgaaatcga
    12001 cgatccatca cagctttcgc cggggcaggg gctattggaa gtcgttacgt atgagccgca
    12061 agcgatcgtg tatgttggcg aaaaacaagg gcgtggggag gtcattgcgt ttccattggc
    12121 cgcgggaacc tatcccatca tcgtagaaat ggggggacaa cgtcttcgaa gagaagttca
    12181 agtaacggct tcgaagcgaa cgcgatggat cgtggaggcg gagtggcagc gttgaggagc
    12241 atggcattgg gctggtggat gctcgcggtg acaggatgct gcctgccctt atcgtcgtcg
    12301 gaaagcacgg actctcctgc ccactcctcg tcgtcctcac caccggcgcc agaagcgtcc
    12361 ggcatgattg tgtcggaaga cctcttggca tcggtggcgc ggtgcgacgt ataccatcga
    12421 ggagcggtca tcgacatggg atcgtcgttg agccatggcc ggttcgatta ttccttcgat
    12481 cagccggcga gtgtgcaaga cgttcaacgc gatggtgcaa catggggccg catcaagggt
    12541 ggtgttgtgt cgttgcgatt cgagcaactc gaggacgagc cggtgtttgt ggagacgcgg
    12601 attcgtggtc tggatgctcg ggcagccacg gtgcgtatcg atggaaaagc tgttggaact
    12661 ttgcgatttg cgcgggaaga gacgcaggtg gtatcgacgg cgaccagctc ggaaccgctg
    12721 tccgcggggc agcatctggt gtcttttcaa ttccatcgtt cgaggaaaga caaacctgtc
    12781 gtggagatgg attggattcg catagggctt cccgatcaag atccgacgac gtatgccgct
    12841 ccgacgttac gtgatttggc gacggatacg atggtagggg agcgtcctta tcgagcgctg
    12901 gcgctacgag caccggggcg agtgagatgt gccattgcgg ccaccgatac gatgcgtttg
    12961 caggcgatgc ttggttacgc tggagcggga gaaggagaag cgcgaatcga aatcgtggag
    13021 ccgggcaagc catcggtgct tctgcatgcg gccaaggttg ggggagaggg acacaggacg
    13081 gaagggctcg atatcccctt ggaaggactt gcgggaagaa tcgtagcgtt ggatttggta
    13141 gccgcatcga cagcgccggg gggaaaggta ttgtttggcg agccgatgct tcgtatccga
    13201 gaccctcgta ttgccgatcg tccggacacg aaggttgtcg ttctcgtggt gttcactggt
    13261 gcgaatcgga cgcatttgcc gggatacgct acggtggaga ccatgccagc gttatcgtcg
    13321 gtggtggaag aatcggttgt ttttcatcaa catcgagcgc cttccaccgt ggcggcaggg
    13381 tccatggcat cgttgctcac ggggtattca cccgctgtgc ataaggtaac cgatagcgca
    13441 acccggatgc cggatcgcgt atccatgctg agcgccatgg gtcgcgatcg acgggtggct
    13501 accgccatgt tcacggcgaa tccatcaacg ttcgaagcgt ttggattcaa tcaaggatgg
    13561 agtcactatc gcgatttctc ccctgtatcc ggggcgtcgc gagccacacc gttgcgagaa
    13621 gccatggcct ggctcgataa cctcttgaaa aaggacaaag atcgtcagat ttttcttgtg
    13681 gtgcatgcac gagggggaca tccgccgtgg gtaggttcgg acgatgattt caaggatcta
    13741 cctccgaaag aatacgtggg acctttgaca ggacgaaggg gggggcaagt gctggcgaag
    13801 gagcgcaatc gacgatatgg gcgggcgccc atgacagcgg aggaccgggt tcgcgtcgat
    13861 gctttcgtgc ggttggcgct tcgagcggaa gatgcgcaac tcggacaact cgtcgaactg
    13921 cttcgtcaac atcgggtgtg gtcttcgacg ttgttcatgg tcacctcgga cattgcgatg
    13981 ggaggggatg ggcgggttcc ctttggtgat ggcgaaccga tgggagagga tgtcttggaa
    14041 atccctctga ttgtgcgttt tccggggcat cgtttcggcg gaagtgcggt ccacgtgccc
    14101 acgaccgcga tggatgttgc gcgaacgacg ctgcagagtc tggggctcga accgccggag
    14161 gccatggaag gaagggattt attcgagatt gcggcacatc cggggcgctt tgcccaagct
    14221 cctcaatttt ccatgacagg ggcggtgtat tcgacccggt gggggaactg gcttctcacg
    14281 ggtgtacctc cacgcgctcc caagttctgc gaggtacaac cgggtctgga gtgtacggag
    14341 gacatggcgg ctcgagagtc tttttttgct tcttggatgt ggcgactgac gttcgatcat
    14401 cggcaagcaa gcgagcgcgt tttcacgcca tcggctatcc gcgagccagc cacattggat
    14461 cccgataccg tggcggcgct cacggtatgg ggcagcctgg agcccgagcc gacaaagagg
    14521 taactactgg atattacaga ggaatattac catgcttgcg agagggattg gactgtcttt
    14581 tgtccttgaa cagttccagc gcatggtgga ttcgtcgacg caacagtctg ggatagatga
    14641 cttcatcgat gaagcctagt tcggcggctt tgtaggggct tgcgaaagcg gttttgtatt
    14701 cggcaatgag cctggcgcgt tcttgctcgg ggtcttgggc ctggtggagg tagttgcgat
    14761 ggatgatgtt gacggcacca tccggtccca tcacagcgat ttcggcggta ggaaaggcga
    14821 tattataatc ggcccgaatg tgtttggagg ccatgacgtc gtaagcgccg ccataggctt
    14881 tgcgggtgat gatcgtgatt ttgggtacgg tagcttccgc gaaggcgaac agaagctttg
    14941 cgccatgaac gatgatgccg ccgaattcct gatcggttcc gggcaggaag ccgggtacgt
    15001 ccacgagggt gaccaagggg atgttgaaac aatcgcaaaa ccggacgaag cgagcggctt
    15061 tgatggaggc gtggatatcg agacatcccg cgaggaacat ggggttattg gccaccacgc
    15121 cgatggttct tccgccgacc cgagcgaatc ccacgatgat atttttcgcg taatcctcgt
    15181 gaatttcgag caggcgccca tcgtcgacca ccgctttgat gatttctttg atgtcgtagg
    15241 gtttgtggga ctcgacggga accatcgtat cgagttcggg gatttctcgt tcgacggaat
    15301 cggagcaagg caagcgaggc gggtcttcca tgttgttgga agggatgtat gacaatagtt
    15361 cgcgcatcaa ggcgatacag gcggcatcgt cgggagcggt gagatggcag acgccgcttt
    15421 tggaggcgtg tgtggcggcg ccgccgaggg cttctttggt tacttcttcg tgggtaacgg
    15481 cgcggatgac atcgggtccg gtgatgaaca tatagcttgt tttttggacc atgaggatga
    15541 agtcggtgat ggctggggag tagaccgcgc ctcccgcgca aggaccgagg atggcgctga
    15601 tttggggaac gacgccggaa gccaaggtat tgcgtagaaa tatatccgcg tatccaccga
    15661 gcgattcgac gccttcctgg attcgggcac ctcctgagtc attcaagccg atgatggggg
    15721 cgccgacttt catcgcatgg tccatcactt tgcagatttt ttgggcgtag gcgccggaca
    15781 gggaccctcc gaagacggtg aagtcttgtg cgaacacgaa gacttttcgg ccatccacgg
    15841 tcccccaccc cgtgaccacg ccatcgccga gtattttggt gtcctgcatg ccaaagtcgg
    15901 tgcagcggtg cgtgacgaac cgatccaatt ccacgaatga atctggatcg agaagcagat
    15961 cgatgcgttc tcgggcggtg cgttttcccg cctcgtgttg tttttcgatt cgctttggat
    16021 cccctttcaa cgctttttcg ttggtttcat ccaacgtgac caactcagga cggctcgatt
    16081 ggctcatatc ccgcgcgtac cacgggatcg taccggacga aaagccatgt cgcgatttcg
    16141 atcgattctc gacaaaaacc tagcgcagca acagcagatc ctgattcaca ccgtcgtaca
    16201 cggcgcgaag cctttcatcg tcacgcatcc attgaagcaa tacttccatg gcggcacgat
    16261 actggtctcg atccagcaat cctcgcaaca attggacccg aagaaaaagc ccggcagttt
    16321 gcacgacatc gcacaggcaa taggcatgta cttgttcgag catgcccgcg tgaatcaacg
    16381 gtcccacgtc tttgccgtcc acccctactt ttccgggcaa tccaatcaat ttggtgaggg
    16441 tatcgagggg cacggggcgc gaagctccgt gatcggtcaa gtaatccatc aggtcgaggt
    16501 gcccttgcgc cgagtaccga taacgcattc cggaaggttg aaagtagtgt cgaaaagcaa
    16561 ttccatggcg caaacaccgg gcgacgatga cgggcaggtc gaagcccctg ccattgtagg
    16621 tcaccagatc ggggcgtcgt tcttcgacga atcgaacgaa atcctcgagg acttccggtt
    16681 ctgtttttcg agggcccacc acgccaagtt tgatgggacg gtattcgtcg ctcatccaca
    16741 acgctccgat agcgacaatt tggtgaaacg gcgcgggagg caaggacgac ccctccgctt
    16801 tggctggcat cggcaacgta ggatccacga tggtttcgat atcgatgacc agaaatgagg
    16861 cgggcatgct ggatagcata catgcaaccg tggtttgggg agagggtccg acggattcct
    16921 ccggcgaaag gttggaggcc gttgcctctc gaccctagag aagataggtc taatcgtccg
    16981 gtcaggactt gaaggttggc atgccgaaca acttgcttgt ggtcaatgtc gagtacagcg
    17041 aaacgcgtgt agcgctgatc gaggagggga tcctcacgga gctacagacg gagcgaacgc
    17101 gtggaaaaac catggtaggc aacgtctacc tgggaaaggt gacccgtgtg cttccgggaa
    17161 tgcaggccgc tttcgtcgat atcggccagg acaaggccgc gtttcttcat gtggaagacg
    17221 tgattcgtcc cgatgacttt gagcaatatt tggcgggtgg ggattcgtac gccacggctg
    17281 atgggtccga atcggagtcg gacgacgagg cgggggtcga tacgaccgta gggggttcgc
    17341 cggagaaaag ccgttccaaa cccgcccggg tttcgcggga aacgccaatt cgagaggttc
    17401 ttcgggaggg gcaagaggtc atcgtgcagg tttccaaaga ccccatcggt acgaaagggg
    17461 ctcggataac cagccatatt tctcttcctg gtcgtttcgt ggtgtacacg cccaccatcg
    17521 atcatatcgg gatatcacga cgaattggtt cggcggtcga acgccagcgc cttcgcgacg
    17581 tcatcgaaac catgcgcccg cccaaagggg ggctcatcgt tcgcacggtt gcggaagggt
    17641 tgctgaaaaa acagctcaag gccgatatcg gatacctcgt tcggttgtgg ggggaaatcg
    17701 ttcgcaaacg agatacgcat ccgaaaccgc cgtgctgcct gtatacggaa ctcgacatcg
    17761 tgctgcgatc cgcacgcgat cggctcaccg acgacatcga acagatcgtc atcgatgacc
    17821 cacagcgatg tgaacagttg aaacgtttcg tagagacgtt catgcccgag cgcgtcgctt
    17881 gtatccagct atacgaaggc aatgagccca tcttcgacgc ttacggtatc gaggatgaaa
    17941 tcacgcgcgc gttgagtcgc aaggtacctt tgccttcggg gggttacttg gtcattgatc
    18001 aggcggaagc cctcaccgcg atcgacgtca acacgggtcg ttttgtcgga aagggctcga
    18061 aggatctcga ggaaacgatt ctgaaaacca atttggaagc tgtggaggaa attgcgtatc
    18121 agcttcgatt tcgcaacttg ggaggcctta tcgtgctcga tgtgatcgac atggatcgtg
    18181 cagccaatcg agagttcgtg cttaaacgtc tcgctgagtt gcttcaaaaa gacaaagcac
    18241 aaaccaccct caatcccgta tcggaattgg gtctcatcga gatgacgaga aaacgcaccc
    18301 gggaaagcct gggccggatg cttcacgaac catgtttcta ctgcgatggc accgggcagt
    18361 tgcaatcccg taccaccatt gcccttgaaa tccttcgaca aatccgaaga gagcacccca
    18421 cgctcaccgg ctatcaagtg accgtcaacg cccatcctgc cgttgtcgac atactcaaaa
    18481 acgaagaacg ggaggctttg gccgcaacag aaaaccgtct catgcgtcgt atcgagttgg
    18541 ttccacgacg cgagtatcac atcgagcaat tcgatctaca gggaaagtaa atatggcgga
    18601 tcagaccacg gatcaggatt tgcggcgcgc agaacggatt accatcaaca aggagttcga
    18661 atcgttcgac gctttcattc aagaatacgt atccaatatc tcgcgctcag gcgtgttcat
    18721 tcgatccaaa acacctttgc cgatcggcac caaagtcaac ttgcggttca cggtcatcat
    18781 ggatgatatc gagaccattg aaggcacagg agaggtagtc cgaatcgatg agacgggaat
    18841 gggtgtcgtc tttcgggatc tcacgggata ctcaaagaat ttgatcgagc gactgcttgt
    18901 ccaacgggtc gattgaccga cggactacgc aacgccaggc agataccctt tggatcagct
    18961 ccctacggtc atccgagggt gggttcagta gcggacatcg gcataccaat gtccgaaacg
    19021 ctcacgaacg agcgtcatct ccgcgccggt tcgagtccga acgcgaaaac ctcgctcccc
    19081 ttccgcgagg cccaccacgg ttcccatgac ctcaacctcg tgccattcgc cggcgagtcg
    19141 aacccggtgg ggccgccaac gatcgtgcgg gcgggcatcc accaccaaat gaatccaacg
    19201 ttccaaatcc gcgggaggat cccaacctcc ttcgacgcga atgtcatact ccgcttgcgc
    19261 gatggctctc gacgacaagg gccatgggct acccgcggtg aacaccagga tcaagtcgta
    19321 gccatccacg aggtgaaccg caaaaaacgt tctttttcgc gcatatacag ccagttcgcg
    19381 caatcccccg agagccccgc tcaatcgttc cgtcgcatgg cgaagctcga tttgtaaatg
    19441 tgccgaagtc aatcgaatct cgaagggttc caacacccca gcatagtcga cacactcccc
    19501 gagttcgtcg accaacgcca caccagccaa tcccgaagag cttccaccaa gttctgcaga
    19561 atccgggtaa atcccgtcgt gtcctggtcc cgcgctggca ccccaggtcg cagcggcatc
    19621 tcattgtcct cgcggatccg tccctacgaa atcatcaggc aacaacagca tcacggtttc
    19681 cgcattacgc tccacactca cgggggactg cgctcgcaac gatgacaaca gcgcttcgag
    19741 ttgctctcgc attcgcgtgg caaggatctc ctcggtcaac atctctttcc ttcgctccca
    19801 aggaagttgc gacgcctcct gcgtggccac ccataaaatg acatgggaac cgaaaggaga
    19861 acgcgacggt gtgctttgat cacccacggc atcgagacga aacagtattt ttacgaaagt
    19921 ggaatcaaag gtggcagggg gttgtcctgg ggcgaggcgt tgcgtcaggt ccgcgacacg
    19981 accgtcctca gtgacaggag gcaggtcttg aatcactacc tccaaccctt ctttgtcgac
    20041 cgcttccgct ttctttttga aagcttccgc gtctttttcc cctcgcacgg catcggcgat
    20101 tcgcgcggcc acgcgatcca ccgctttcca atcggcttcg ttatccggat ccttgggcat
    20161 gaccaccgcg tgggtcgatc gacgcgccac gggacgatcc agatctagcc aatgcatttg
    20221 cgtatgtttg gccacttcct cgtcggtgat cgcatgggct ttcgattctt gtttcatctg
    20281 atcgatcaac gctcgggcca gcaccgctcg acgttgtacc aaaagcgcgg gagtcctatc
    20341 gagacctcgt tcatgggcgg ctgtagccaa aagagcatcg aatacgagat gggctcgcgc
    20401 ttgggcaaca ggaaggttcc tggctttcga caactcacga acctcgtcca tcgtgatgat
    20461 ctgatcgtcc agctgcgccg cgacacctcg atccaacgtg gaaacacgct cacgatgggc
    20521 tgcggattca gacgccgtac agccggcaag caagcccaac agcacgccca acatcggcgc
    20581 atacccacga tcaacggctg tcatcccgca ctcctaggtc gaagcagatc aaccgaggag
    20641 cgaagcaaca ccgctccaca agcaaaacag tatatcgcgc tgagatcgtc tcggtgaccc
    20701 aggacataaa tgccaaaaac caaaacgcct acggccatga caagcaatac cagcgccacg
    20761 atgtcgagca ctcttcccat gcgttcgtcc aaccatgcga ggtccgaaat ccatcttccc
    20821 atacctattc gcctagacca agcccgttag gccaagcagg aaatctcgga cgactacagt
    20881 catcgcaaac gcttgcggcg acgttcttcc gccaggtgtt gcgtcattct tttctgatct
    20941 ggaagctctt ccaccaacgt ccataggtct tgtccgccgt gttcctcgca aacctccagg
    21001 atcaaatcca gggctcgaag cgcgtcgagc gcatcttgat gacgtccggg gcgttcgatc
    21061 acggccattc gcacacgcaa accggtaagc gtgtcgatca tgcgatgcat gatctcgagg
    21121 tacacggttt cgtcgaatcg gctcacgggt cccaatccga aaagcaggag tttttcaaaa
    21181 ggaattcgtg gctttccgga gatgaggacc acttctcccg acttgcctgt cagcgcctcc
    21241 gaggccatcc atcgcgacaa tcgtgcggcc atccgccaat ccagcaaacc cgccaatccc
    21301 cgcacggggc gttcatcctc gaacaggcag catgcgagca cctcgctgcc caccgagtcc
    21361 aactgccgca ggtcaggact gacgaatcga aaatccatgc cattaccggt ccaagtcgac
    21421 ccggcctact tcttcggcaa tccggtcgag cactccgttg acgaaggcgc cggaatcctc
    21481 ggaaccgaat agtttggcaa gttcgatcgc ttcatcgagg atgacggcac gcggcacgga
    21541 tgtctgctcc agcagctcgt aggcagccat tctcaacagg ttccgatcca ctcgagccat
    21601 tcgctccagg cgccaattcg tgctggacgt tcggatgagt tcatcgacac gttctcggca
    21661 gttcatgacg ccgcgcacca aggaatccgc ataggttctt ccttccggat ccgcttcgaa
    21721 ttctcgccaa aaactacgga tttcggcttc gatgtccgcg tccgtcacgg catcgagccc
    21781 aaatagcatc tgcaaagcag cctgtcttcc actggtgcgt gcacccatgg cgcgtcccta
    21841 tagcactcca acaccatccc gtctcaagca tttaaggcag ataatagagc aaggaaagga
    21901 ctccggctcg caccgcggaa cgatggtgcg aagctcccgc gcgagacaat acctgttcgc
    21961 gatgctgcag ataatcatca taggattcgc agatcatatc ttcattggaa tgacgcggat
    22021 gaaatcccaa tcgctccttg gctttcgttc cgtcaaaaaa catttctctt ccgtacatca
    22081 gcgcatgata agggccgagg ggagacaatc ccaaagcgct cgtcagactc atggccgcgg
    22141 tcgtcagacg catgggtagc gatcgaagac gagtgccggt ggccgcgtga ttcacgagcg
    22201 ccgtcagcag ggcacgcatg gttccgaatc gttcggcgcc gatgtgaaac aaatccgggc
    22261 cctgtcgttg cccagccaga atacatgcat cggccaggtc atccgcatgc acgaattggt
    22321 atcggttgtt gcctcttccc aacacgtaga taggttttcc ccttctcacc aattcaaata
    22381 gtatttgaaa aatacccaag cgaccatgtc caaggatcgt ccttgggcgg atgatggtta
    22441 catccaagcc gcggtcgaca taggattcca cgacttgttc ggcgcgatgt tttgccttgc
    22501 cataagcttc ttgaggatgc ggtggttgct tttcatcgac gggattggtt cggggtacac
    22561 caaacacggc gctcgaggac acgtgaacca ctttgcgcac gttttcgcgt cgacaggcct
    22621 cgagcagcac ccgggttccc cccacattca ccgtttcgaa caaatcacga tcttttgcga
    22681 ggggtacttg cgccacattg tggtgaacga ccgccatccc ttgacaagct cgtcgcaccg
    22741 catgttcgtc tcgaatatcg ccttgcatga attccacatc ggcgggccga tcggcaacat
    22801 ccacccgatc gaagacgcga acccgatgcc catggcggcg aagatgactc accagcaaac
    22861 agccgaaata gcctgacccg ccagtaacca aaacgggttc ggagcggtgg ttatccggat
    22921 gcaacggcaa cctcctttac aatggctgcc cgtgggcgcg acaccaggcg atcgaacgaa
    22981 tcatcccttc ttccaactct atttccgggt cgtaacccag atcccgctgt gcggcctcaa
    23041 tcgacaccgc aatcgtggcc gcaagctcgc cggctacgtg aatctcttgg ttatacaagc
    23101 cggacatctg catcaacgag tccacgaatc gagccatgtc cgaggtcacg cggggcaagt
    23161 atcggggccg aacggcaacg tccagaacac gagccacggt ttggatgatt tcaatgaacg
    23221 aatagggacg acgatccgcg atccaatagg tttttccccc cgccttctgc tctttttccg
    23281 ccagcagcaa cgcctgcacg atgttgtcga tatagctcat gctccgcagg ttatcgccct
    23341 tgccgacgat gatcggatga cctcctttga tcatctgaaa aaatcgggac tgccgttcag
    23401 gctgattggg accgtaaaac cagcacggac gaaggatgac cgtctcgatc tttcccgaac
    23461 gatgagcatc gttgatcaac cattcggact gcagtttcga caatccatag ttcaagtaag
    23521 gccgcggagg atcttgctcc accatcaaaa tcgatggcgt agcgttcaaa cccgccggtg
    23581 agttggacga aacgaataga aatcgacgta cgccagcctc aacggcctga tccaaaagat
    23641 tctgggtccc gcgaacgttg atatcgtaca gatcctggat tcgatggggg tgaatgattc
    23701 ctgcacagtg aaagaccgtg tcggcgtctt tgcacaatcc cacgagagaa cgccgatcgc
    23761 gcaggtctgc ccggacaatc tcgaccttgt cgcccaaggc caacaggggc gcgggatcgg
    23821 cctggggctg cacgatgcat cgtacccttc gcggttccgt ggaaatgctt cgaaaacgtg
    23881 cgccttgcac aagcgcctcg gtcaatgatg tgccgagcca tccaggggca ccgctcacga
    23941 cggcgagact catttgcgtt cctctcgaac cggtgggtct tcgcgattgg cgtaccggtc
    24001 tgtttctccg ccctagagcg aacgggacgt caggtcaacg aaggacagcg accccacgga
    24061 acggttcccc ttcgtccacg cattttgcac agcgttttgc caaggttctg gattaccctt
    24121 tgggcccatg ggctcttttc gccactggat cgtaggttct gccttattca ccctgggttc
    24181 tttaccaatc cttgcgaaag cgcagacacc atcgctcgat gtacggcatt ttcaacctcc
    24241 cacctcaccg gatgggtcgc tgtacgtgga aacgaccaac agtcccggag ctagtcagtg
    24301 gaatgtgggc tcatggttgt cttggagttt tcggcctgtc gtgctgcggg atggcaacga
    24361 tgacgtcgtt gccaatctca tatcccagca gctatccctc gaccttttgg gaagtatcgg
    24421 agtgacacgc catgcggcca tcggtctagc cgtaccagcg gtccttgctc agacgggcga
    24481 ttcgaacgag tacacgaaca ctgtcctgca cggatccacg ctttcgagcc aagcattggg
    24541 ggatattgcg ctcaccggaa aggtgacgct gatggctatc gagccgatgg gggggtgggg
    24601 gttcgccgca ctggcccgtg tcactgcgcc aacgggcagc cgaaccgctt atgttggcga
    24661 gggagcactc acgagcgaac tacgtttcct ggctgaatac gatctgctcg ccgcttctgt
    24721 cctagcaacg acaggtttca aattgcgcac gcaggagcgc acgttcgctg gagtgacgta
    24781 cggcaatgaa atcccttggg gaataggggt caaggtgttg cctcaagcca tcggactcga
    24841 tgattctggt cgttggacat ggagcctcga ggcccatgga gcgctgcccg ccggtcccgc
    24901 taacccgttt tcatcggtgg cgctcagccc cgcactgctt ggagcctcgg ctcgttatcg
    24961 gatcggaacg gaccttacgc ttgccggtgg cgtggaggct cccctcgacg gggcggtggg
    25021 ggtccctcta actcgtgtcc tcgtgggcat ctcttacgcg ccacgcaatc atgacatgga
    25081 cggcgatgga atccccgatg acatcgatca atgccccgaa ttggcggaag atttcgacgg
    25141 cttcgaagac gacgatggat gccctgacta tgacaatgat gccgatggcg ttccggatgc
    25201 gatggatcag tgccccaacg agccggaaga cctggacgac ttcgaagacg atgacggatg
    25261 tcccgatccg gataacgatc gcgatggtat cctcgatgaa aacgatgcct gtcccgatgt
    25321 gtggggcttg ccgtcgcaag accccagcaa acatggatgt cccgttcttg accgcgacag
    25381 tgatggaatc cccgacgacc tggaccaatg ccccgacgaa cccgaggaca aagacggatt
    25441 ccaggatgag gatgggtgtc cggatctgga taatgatggg gatggaatcc ctgatgccga
    25501 ggaccagtgt cccctcatcc ccggaccagc ttcgccagcc acgaaatggc atggctgccc
    25561 agtgccggac caggacgggg atacgtttga cgacgaacac gacaagtgcc cccaggagcc
    25621 agagacctgg aatggtgtcg aagacgatga cggatgtccg gatcaaggag ggctttggct
    25681 agctcgcgag caaacgacgc gggagggccc ctcgctggtc attcggactc ccattcgatt
    25741 cgtaggccca acccatgctc cgtccattca cgccgatagt gttcccaccg tgcgtgcgat
    25801 cgcgactatt ctcaatcgcc atccggactg ggtgttggcc gtcggtacca aacccaaccc
    25861 gatcgacggt cctcttgatt ccacccactc gctatcgcgc gcattcgcga tcgtgctggc
    25921 cttgcaatcg tatacgtttc gtgatggggt cgccgaaacc gtgagttggg aagccgttcg
    25981 aacccaaccg ggggcaatcg cgcaaggtgt tggatttctt gtactttatg gtgctgcgga
    26041 gacaacggca tccgaccaag aagaggctgc gccgtgaaac gcaaccctat ttcggcatgg
    26101 tgtgtggggg cggccttact catggcggca gcaccgacca ccgcccgtgc ccaagcttct
    26161 gacgataccc aagagagcgt ggcgtcgcgg tcgtggacga cgagatttgg tccgaacgtt
    26221 gcccgtcgat ggcttcgatc agggcgtttc gaagatcgac tccgtgccgt gcggcgaatc
    26281 ggcagccaaa acgattacac ctcggtgcag attcttgttc aggcattgac cgatgaccca
    26341 gcgttggcat catccccgca aattcgcctt gaaagcgtac gcgcgttggc tccgtttgtg
    26401 tccagagatc ccgtgcggat actgctcacc ggatggatga cgccgccgaa caatcagctt
    26461 gcgaatcccc tgatcaacct tgccgccgat caggcgtcga tggccttggc tgcgtcgggc
    26521 aatacacgag ccatcgaaca gttggtcatg gcccttgcgg aaaatgatca gcccagggcc
    26581 gtgcaagctt tgcgagcgca tccaccagcg aacctcgaac cattgctcca agcgcgtttt
    26641 cgcgagtcga cgcaggtgat cgacctgatc ggacatctcg gggatttgcg agcgattccg
    26701 gcccttcgga agcttctcca caacgcggat ctcaacgtac aggttgccgc agccatcgct
    26761 cttgcccgtc taggggacgc gtccggctgc gggaaggcga aagactggat tcgacaagag
    26821 ggttcgacac acgcgttgag agtcggtgca acccaaacgc tggtgttgtg cagagacccc
    26881 tgggcgcctc gagcgatggc gattttgctg gcagatcccg cgacgcgaca acaagggctg
    26941 gatctcgtgg agatcgctcc aacggcccag tgttcaccaa ccctcgcggg attgctcacc
    27001 attgcggaag atagggagcg cgtccgggtc atcgctgctc tcgctcgttg tggtggcccc
    27061 cttgccgccc ggacacttga atcgttgctg cgcgatggca ataccgatgc cgctttcgcc
    27121 ctcgcgcaat gtcgcgggcc ggaagcctcg caagcgttga gtcgagcgtt ggcggaccca
    27181 aaaacgaaaa gactggcggc ttgggcggga gtgcttcgct atacggagat ggcaatcatt
    27241 gccgagggcc tcggggatgc gctcacatcg ctggcgtcgt cccacgcgcc tgccgatcga
    27301 ttcattgggg tgtttggact tgttttgctt ggggatgtac aacgaatcga ggcggcgcag
    27361 gatcccgttg cggttgtcgc cgcgtgcaat gccgcatcca ctttgtcggc tgacaaaaga
    27421 gaaccatgta tcgaaaaact gaagggcacg agggatccgg cgatgcgtga tgggctcgcc
    27481 ggctgtttgc acgatatcga ctccatcgat gggctttcca cggccaccct tttcgagtgg
    27541 gcggaaagcg aaaggatctc agctcccgtt ttcggccggt tgctcggcgt cagagatagc
    27601 gaatcgtcgc gcaaacgcat cgaacgcctc ctcgcctcca gcgacccgga catgcgggcc
    27661 caagtcgcgt tgggccttgc ccatagtcca caaccttctg ctctttcgct gctcggcgag
    27721 gccctgacat gggaaaccga acccatggta cgtcgtgcca tcgtgaccgc ccttcagagg
    27781 aggcaaagca ccctcggaca aaagtggctg aaccaggccg cgcacctcga tcccgacccg
    27841 atcgttcgct cgctcgcgcg cccacacccc ttcgctcgcc caatcacgca acaacaacaa
    27901 tggttttggc tgcggctcgg cgagtcgcaa gcgacgcaaa cggcgtctca tgcagtgcgc
    27961 gtgatgctgc cccatgggag cgcgtcggtc atcattaccg cggaggatgg aatggccctt
    28021 gtccctggcg taacctatgc ggaaacgcgg ctacgggtcg acggcgtgcc ttggcagttc
    28081 acgtcatcac gaacgcagga ggggcaacga tgagtcccaa aaagcaacct accgacgaga
    28141 acgctacttt cgaacaatca ctccaacaac tcgccgagat cgtcgataaa ctcgagggag
    28201 gggaccttcc gctcgacgaa tccgtggcgc tcttcgaaaa agggatgaaa ctagcgaaac
    28261 actcccaagc gttgcttcag cgagcggaaa agaaggtgga agagcttctt gccgtggaac
    28321 ccgacggctc tcccatcact cgtccgtatg aagaatgagg gctgagcgtg ggacttcgtg
    28381 ctcgagcgca tagacctatg gcggccggtg ggaaagcaag ggatacggaa acggaggagc
    28441 aattgcttcg ggaaccgatc ctgactatag gggagcggca gtgccatgac gaatcaagat
    28501 atcgcctcaa aaggtgaagc tgagaccgtg caaaccgagg aaacggcggc atcccaagac
    28561 cgaattccct cgaatattcc gtcaaaagag atagcggacg caccgagcga taacgagcca
    28621 tcgccatcgg aatccgaagc gtcgggcgaa aaagctgggc cacacgaagc cacagcggaa
    28681 accgtcgagg aatccgatga ggaagccatc gccatcgaag aggaagtgat cgaagatccc
    28741 ttgcaggtgg cgcgggatga aaacaaggcg ttgcgggagc aaatgcttcg attggctgcg
    28801 gatttcgaca attttcgcaa acgatcgcga cgcgacatcg aggatgcggc acggggtgcc
    28861 aaaatggacg tgctgcgaga cctgcttcct gtatttgaca atttggaaag agctgaaacg
    28921 catgccaagc aggcgacgga cgtgcaggcg gtggccacgg gggtggcgat ggtcattcgt
    28981 cagttcgagg atattatcgg gaagtttggt gtcgaacgcg tgaaatccgt cggccaacct
    29041 ttcgatcctg tcatccacga agccatccaa atgcttgaaa cgaaagaaca atctcccggg
    29101 accgtcatgg cggaggtgct tggggggtat cgttggggcg accgtttgct tcgcgcggcg
    29161 atggtggtgg tttcgaaagc tcccgcgcag gagcgtgctt cgggcgaaca cgagacagcg
    29221 tcggacacgg aggcagcggg ggagcaagcg gagcaggcag agtcggaaca agattccgaa
    29281 aagagcgagt cgacgggcga atccgcggtg tttgaacaaa acgacgagga ccccaccgcg
    29341 gagagctagt caccattcat ccaacatcgg ccgcgacagg ctccaaccga tggagtatgc
    29401 ttggcacgcc aatggggaag ataatcggta ttgatcttgg gacgaccaat tcgtgcgtgg
    29461 cggtcttgga agacggggcc gcggatgcga agccagtggt tattcctaac agcgaaggcg
    29521 ctcgaacgac accatcggtc gttgccttca ccaccagcgg ggagcgactg gtgggtcatg
    29581 tggccaagcg gcaaggaatt accaacccgg agcacaccgt ctttgcggtc aaacgcttga
    29641 tgggaagaaa attctcggac cgggaggtgc gtcgtcagat cgagttggct ccttaccggg
    29701 tgatcgaagc gagcaatggc gacgcttgga ttcgaggcag ggatcgggag tattcgccgc
    29761 cggagatttc ctcgattgtt ctcgataaaa tgcgggaggt tgccgaaacc tatctgggcg
    29821 agaagctgac ggaggtggtg gtcacggtgc cggcttattt cgatgatgcg cagaggcaag
    29881 ccacgaagga tgccgggcgc atcgcgggat tggaggtcaa gcgaatcatc aatgagccga
    29941 cggcggcagc gcttgcgtat gggcttagct tgaaggagcg cgaaaccatt gcggtttatg
    30001 acttgggggg cgggacgttc gatatttctg tgctgcagat cgagggggga gttttcggag
    30061 tgcgagccac cgggggcgat acgcatttgg ggggtgagga cttcgaccag agaatcatcg
    30121 acacgctggc ggaagagttc gagatggaaa accaggtcga tcttcgcaag gatcggatgg
    30181 cgttacaaag attgaaggag gcagcggaaa aggccaaaca cgaactgagc agttcgttgg
    30241 agaccgaaat caacatccct ttcatcgcca cggggccgac ggggccgttg catttgcagc
    30301 gcacgatgaa gcgttcggac ttggaaattc tgacctctga cctgatcgac cgtacgctcg
    30361 atgcctgtcg tgcagcgctt caggatgcga agttgagcgc tcgagacatc gacaatgtgg
    30421 tgttggtcgg gggcatgacg cgaatgccag cggttcaaaa atcagtgaaa gagttttttg
    30481 gaaaagaacc ttgtcggagt gtcaatcccg acgaggtggt ggcggtgggg gcggcgattc
    30541 aagcagcggc gttggcagga gaggtcgacg aggttttgtt gttggatgtc gcgccactgt
    30601 ccatcggtgt cgagacggga gggggcgtat ttttccggat gatcgaaaga aatcgcacca
    30661 ttcccaccga ggcctcgggt gtgttcacca ccagcgtgga caaccaatcg ttcgtccccg
    30721 tacacgtgct gcaaggcgaa agggagatgg cggcggacaa taggagtttg gctcacttcg
    30781 aactcaccgg aatcccaccg gctccgcggg gtgtacccaa aattcaagtg acgtttcgag
    30841 tcgacgcgaa cggcattttg catgtggaag cgcgggatct tggaaccaac cgatcgcagg
    30901 cgatcaaggt gacgccaacc agtggactgt cgcaagatga aatcgatcgg ctagtgcagg
    30961 agggagataa gttccgcgaa acggattcgt tgcggaagga actggcggaa atgcgtaatc
    31021 aggcggaaac gttgttgtac accaccgagc aagcgttgga cgggtacgcg gacctgctcg
    31081 atgagcaggt ggtggccgag gtgagaggtc aagcggaaac cttgcgtgga ctattgaaat
    31141 cgagtggaag ttttgaggat attcgaagcg cttatcaaga attggaatcc gcggccttta
    31201 gaattgccga gtccatgtac ggatcggaat cctgacaaat ctgtaaccat gtgcgttcgt
    31261 tgagaatttt cgtttttttg aaaaagtggc cccggaaaag gcttgtgcgg cttgccgttg
    31321 ccaaggggca tcggatacaa ggaaggacct gattgggttt tcccttgtca gggagtcagc
    31381 ggttcgttgg tcgaaccgtg tcgtttcgtc taatcgcgtt cgagtcggct ggtgtgctgg
    31441 cggaggcatg gatggtcaat ccccagatgg tgatgtacga ggaggagttc aaccagattc
    31501 agacagtcgt agaccgtctt gtcagggacg cgaacgctaa agtcgtgttc atcgtcgaca
    31561 agaacggtca gctcattgcg gctagtggtg acgtcgagaa tctggatacg acatctctgg
    31621 cttcgttgac ggctggaaat attgcggcga ccggaggtat ggcgaaactg ctcaaggaaa
    31681 acgagttcgc gacgcagttt cacgaaggcg aaaaagccaa tatccatatc caactcgtag
    31741 gtaaccgcgt gattttggtg gttatcttcg acgcgcgttc gagcttgggg ctcgtacggt
    31801 tgcgggtgaa gaaggcgagc gatgaactca accgcatttt cgaggcgttg ttacgaaaag
    31861 tccaagagcc aggcggtgaa tctccttttg ccgaaatcac agacgaggat atcgataacc
    31921 ttttcaacga ctaagggcgc gcgatgagct tcatcaacta catggcgcgc gagatcaact
    31981 gcaagatcgt ctattacggt ccaggcttat gcggaaagac gacgaacctg cagtatattt
    32041 atgatcggac caatcccgac gcgaaaggga agatgatctc gctggccaca gagactgagc
    32101 ggacgttgtt ctttgacttt ttacctttgt cgctgggcga aattcgtggg ttcaagacgc
    32161 gttttcattt gtacaccgtg ccgggacagg ttttttacga cgccagtcgc aagcttattc
    32221 tcaaaggggt ggacggtgtc gtatttgtcg ctgattcgca aattatgcgc atggaagcga
    32281 atatggaaag cgtggacaat ctacgagtca acttggctga gcagggctac gcgcttgaaa
    32341 aactcccata cgtagtacag tacaacaagc gtgacttgcc ggcggtagcg gacgtcgacg
    32401 aactccggga aatgctcaat ccggatgggg taccggattt cgaggcggtg gcgcgcacgg
    32461 gcgtgggggt tttcgaaacg ctcaaggcgg tggcaaagtt ggtattgacg gaactcaagc
    32521 ggggaggctg acactcatgt ggcctccctt tccatgaaaa actccctttc cattaatgga
    32581 tagcctgctg ccgtgggact tcggtcaccc ttggctcgaa ggagctttgg ggaagggtct
    32641 tttcggtggc tggggcagcg agggctgcgg ggataccgac gaacctttgg agcaggtctt
    32701 cgggcagcac caagaaaatg gtgggggatt catggttgca aagttggcgc tattggttcg
    32761 ggggaggtag gataattggt gctggcaagg cgcttaccaa tgaagagggt tcggagatca
    32821 acaatgaacg gcgcgtggaa gcgtggatgg gtgttgtcgg tgatgatggg aggtatcatc
    32881 acctcgatcg cctcgtgctc ggaaaacgaa tcgagcattt tcatccggca ggttctcaaa
    32941 ccctcgggcg cggactgttc gttttccgcc gagccagggg gatcagcctt atcgacgggc
    33001 gtgatggatc tggcgtttac cagggaatac caagcggggt tattgctcgg caaccaattg
    33061 gtggagcgag gatcagcgga tcagctacgt acggaaacag ccagattcct cgccgagggc
    33121 gcagaggtgg agctcgaaac gaccgacgga aaggggatca actcttttac cgtcccagtg
    33181 agcggcttcg tcgatccaag cacagggacg gaaccgggct ggggggtggt gacagctacc
    33241 ctgatcgatt ctggaaccgg acagggcttg gcggcaggat tcccggaagg tcaaagaagt
    33301 accgtcgaag gattggtcgt ggcggtcgtc aaggtgtttg ggcataccct cggtggaaag
    33361 gaattggaca gcggaaagta ccggtttcca atcagtattt gttatgggtg tctcgtcgac
    33421 tttccaccag agtcggtgga cccggatctt ccgcttccga attgtgcgaa tgtgtcgaaa
    33481 tcgacgggta cggttccttg catgattggc caggatgaac ctgtcgattg tcgactttgc
    33541 caggcccagg gagcacctgc ttccctctgt aatccttgac caacaacacc cggaatcgtt
    33601 tcgaaaccga cgtttcacgt gaaacgggca agggggcgtg acagcgcttc gcgaaaccga
    33661 tagtctgcgt gccatgcgac cgtgcaagct cctggctctg cttattatcg ccacattatt
    33721 ctttggctct ttcgcctctt tcgcctctta cgcccacgca cagccgcaat ccaatctggc
    33781 cgtcacgctc ggcgcggccc ttacgcaaaa cccgtccgct acgcgtttcc acaccgcatt
    33841 gcacggcgat attcttttcg gacgcaccag cccttgggat tttggcatcg ggccttacgc
    33901 cgatcttgcc accgcctgga acgatttttc cgtgggtacc ggagcttccc tgctcctacc
    33961 catccacgat gcccttccga ttgtcctttc ggctggaccc tttgttcgcg cctttgactc
    34021 ccgcgagtct atcggtcttt ccaccgacct attctggggc tcccggagct tcaactacca
    34081 cggctcctac gcaatggctt ttggtctgat cttgcaaggg cgtacggcat tcggggccga
    34141 gcccgatcga gccctcatcc ttggatttcg catcgacggc caagcttttg cgctcccgct
    34201 cttgctcgca tacggcgcca tcaccggagg cgagccgtga gcgatcgtcc gaaaaggcta
    34261 acctccattc gcctcgccgg acggcaccta agggtccgca ccaacgcctc ggaccatcac
    34321 ctggcccgaa tcgtcggcat gatcgaagcg cgggctaacg ccatcgctgc tggaaagccc
    34381 ttgacgcctg acactttttt actcgtcgcc atgtcttttg ccgaagaggc tgccgaggcg
    34441 cgcgctcgcg cggaccgtat cctttccatg gcgcagcaaa ccgcccaaac gctccttaat
    34501 cgcgtcgaat ctgccctcga cttgccatct gagcacgacg aaagcgccga ccttcaagca
    34561 gaatccatgg ttgacgacga tctcgagtcc tgcgaacacc aattaatcac gccagatccg
    34621 aacgacgcaa cgtagtgtgt gccctattct acataaaacg acgccccaat cccccggtat
    34681 gtttcacgtg aaacaatcct cacgctccct acgcccaccg ccccttctcc aatcgccaaa
    34741 tctgacgctc ccttatgtca aaatacgatg gatcatgcgt caccacaatc accacccgtt
    34801 cagacgccac ctcctgccgc actactcgca cgatcgccgc gaccccttgc tcgtccaatc
    34861 ccgtcgttgg ctcatctagc agcacgagcg acggtctatg caacaacgcc cgcgcgagcg
    34921 ataccctctg cctttgtccc cgtgatagct tcgacaccat acgatccctg accgccccaa
    34981 gaccaaatct attcatcgcc tgctctacgt cttgctcccc cgctgcgtat agccccgacc
    35041 caagccgcaa attctcctcc actgtcaact ccccgtacgt cagcaactcg tgcgacacca
    35101 acccaatcgc ctccctaatc cgactcctcg ccacgccctc atattcgact tgcccaaacg
    35161 acggactcaa tagccccgcc acaatccgca gcaacgtcga cttgcccgat ccattcggcc
    35221 ccaccaaaag atgcaatacc cccttttgca tacgaccgct caacccccga agcacggccg
    35281 ttccccca
//
LOCUS       DAOSOR010000004        31347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_1465_length_31347_cov_5.129618, whole genome shotgun sequence.
ACCESSION   DAOSOR010000004 DAOSOR010000000
VERSION     DAOSOR010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 31347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31347
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1465_length_31347_cov_5.129618"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..585
                     /locus_tag="PK710_00485"
     CDS             259..585
                     /locus_tag="PK710_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HOT08207.1"
                     /translation="MTKADIAHIIYTRIGGFSKKESTDLVDLVFETMKETLGRGDKIK
                     ISGFGNFVLRDKRKRQGRNPQTGEPITITERRVLSFKPSQILKQTLNDAPTASSTEGS
                     SPNRHP"
     gene            604..984
                     /locus_tag="PK710_00490"
     CDS             604..984
                     /locus_tag="PK710_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOT08208.1"
                     /translation="MSSDGTQIRKLYYRIGEVARLLGVETSAIRHWESEFPSLRPRRT
                     KTGQRMYSQHELERLKEIKRLRFDLGYTIRGARMALRNKGLEAREPQDPVVIDNEQLR
                     DTLLDLHQQITSFIDALERERNQA"
     gene            981..1925
                     /locus_tag="PK710_00495"
     CDS             981..1925
                     /locus_tag="PK710_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08209.1"
                     /translation="MSARTALVHGTMASLLVASATVLPALAFAQQDASADAFDAEVAM
                     DTASDALESADAEQEPTAATLGPETSLVISEGVNLRFSPPEGWMSVESKPVDIPDLDK
                     IPGAQALFKHTWTAPHRRKAMAPRMSVVCATAPSDSWTRGLESRVFEHLTSVAHNELS
                     EWMSVDSLQSGPIDSTSPLFFQSFEAKGRAGGQRKTGALRVLEEDAIDNKRAPFVGVG
                     RHTLGFLANPDRVFVCSMACIEPAWRETAICPTSVASLHIDGALAPEPSSSTLGRLAI
                     GIQRRPSALVGMAAGLLLAFVGTLAVLRGSLLRPASKS"
     gene            complement(1920..2636)
                     /locus_tag="PK710_00500"
     CDS             complement(1920..2636)
                     /locus_tag="PK710_00500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT08210.1"
                     /translation="MTTTPTVSIVIPVFNEEAILRAAIVDLREKLKEAPWTFEIIIAE
                     NGSTDATVAILAPLAAKYEEIRWFSLGEPNYGLALRRGILEAKGTFVVCDEIDLCDTV
                     FHESSVALLQHGGVDLVIGSKLLSGSSDERPWARNMASRVYNGLLRTTLGFRGTDTHG
                     LKAFHRERLLPVVHACVVDKDVFASEFVIRAYRKGLSIREIPVRVVEKRRPSINLVRR
                     VPNVVKQVLRLAWAIRIHDS"
     gene            complement(2633..3937)
                     /locus_tag="PK710_00505"
     CDS             complement(2633..3937)
                     /locus_tag="PK710_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT08211.1"
                     /translation="MSSKQPSVVVLGAGLTGMSASYHLQSMGIGHRLLERAPQPGGLA
                     ITVEQSGFRFDRTGHLLHLRDPAMRSLATAWLGNEWLSIERRSMIWSHGAYTRYPFQA
                     NTLGLPAKVAYECIVGFVHALRQPHAQAPTDFEQYCLQHFGDGFSKHFMIPYNSRLWG
                     VHPREITAEWCSRFVPLPTLEEVIAGAVGLAGPEMGYNASFLYPRKGIGALTSALAKA
                     VGPVECGKELVALDPQRKRLTLQDGEQIDYDVLISTIPLDHLLARTEGIAEPIRQAAS
                     KLRCSSLHYLDVALDVPSAHPFHWVYVPEQRYPFYRVGVYSNFSKEMAPEGKACMYIE
                     LADRQPPILESLVPDVAAGLVEMGFIDQPNQVLFAEHRTIEHAYVIFDHAYYASVDSV
                     HGFLKDQDILSTGRYGGWNYSSMEDALLFGQAAAHWARKSLP"
     gene            4238..4948
                     /locus_tag="PK710_00510"
     CDS             4238..4948
                     /locus_tag="PK710_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08212.1"
                     /translation="MNMRLALVPLCLLSAVSVMGCTAPEEDSCNIKTGGIYVEYEVFE
                     EGNQARATATFWVGDKPGGTYLSLGSCGDNITVNGQGMSKKTDNVNHDYYEAKLGKAD
                     SYEFVFTREDEDPYSSSVSPRKPVNITAPTSGTSISRADAFEITWENNDSGKIELLIE
                     GDCIEDFPSVNGDDVADTGSYSVNADGIKPFMKDDADKTCSASLSLTRKNSGSLSPSL
                     KGTIKGYSMGHTSFTSTP"
     gene            complement(4909..5673)
                     /locus_tag="PK710_00515"
     CDS             complement(4909..5673)
                     /locus_tag="PK710_00515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOT08213.1"
                     /translation="MSIVRVPLAGIEPGARRLDQEAAHYIRHVLRLQPGDRLQVFDPE
                     QATEAEATVVSIHRDRVVCELSDVKTASCVPSTPAWLLLGLCKTESFAWALREATALG
                     VTDIVPLMLSRSASSTHRPDAKRMVRWRKILVQGARQCGRGNIPILHDVCTLEQSLSR
                     VPDSLVRLCLWEKATEPVGKWIRTARNGVALIIGPEGGIENEEARQAEASGFILGSLG
                     GLILRTETAVLAALGAWHLSREESQGVDVKLVCPME"
     gene            complement(5670..6539)
                     /locus_tag="PK710_00520"
     CDS             complement(5670..6539)
                     /locus_tag="PK710_00520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOT08214.1"
                     /translation="MNASSPPRFPRVALDVPAPLADEISYGLFDLHATGVEIRDHTTW
                     EQARVDGTVTLLASFSSDDEATQAIAHLAAEWNPRRDDVLGDAWRDSWKEHFQPFLLT
                     PHIAIRPPWAPAEPRPGVHWLEMDPGRAFGTGLHPTTRLVAQSMDNLAPRLTDCTILD
                     VGCGSGVLSLCALVLGAKHARAVDNDPDAVAVTLENALRNDLAHRITADTSDVATIES
                     SYPFIVANIEARVLIPMAEPIVRRLAPAGIVVLSGILRTQVDEVIAAYGALRLRQTMA
                     ESDWVAIELESVR"
     gene            complement(6545..7474)
                     /locus_tag="PK710_00525"
     CDS             complement(6545..7474)
                     /locus_tag="PK710_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08215.1"
                     /translation="MSTKPLSHRISIVGLILFLSSTLLSISPCPAFGQSSRPASQTDL
                     IRSGVQLFEDQRYEESIQTLSAALLRPTTSQSDRIDVYRYLAFNYLVLSQMDEAEAAV
                     RGLYVLDPQFSLADTESPRFRSFFKEVRERWEAEGRPGLERGSAALAAPVIKHASPAE
                     WERGKPVPVTGQIADPNHRVASIVVHVRSGSKGKYRHLEARLAQGVFRTSIPGPMLTP
                     PLVEYYIEALDEAHLPIASRGDAAAPLRIAIPEPEKGGSLFASPWFWVATAAVVAGGV
                     TGAILLSDTKTDSQKPPPQQPAPTSRVVIVIGN"
     gene            complement(7564..8277)
                     /locus_tag="PK710_00530"
     CDS             complement(7564..8277)
                     /locus_tag="PK710_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT08216.1"
                     /translation="MSRTVIVGDVHGCCAELQELLARVGFSDDDHLYFVGDLVGRGPD
                     TLGVLALAQQLGAVAVRGNHEQKLLSFVQSQREGGPLVRLGPGHLQLVHSLSEQDWAW
                     LEAMPLWHDLPEHDLRIVHAGVKPGVPIEATDTGILLTIRAITQRGQASITRGGEPWA
                     RWYHGPPHIVFGHNALMHPQLHPWATGIDLGCVYGGFLAALVLGPGQTIPSIEERQEL
                     LVSVSARRAYYPVRKNYGR"
     gene            complement(8338..9609)
                     /gene="glgC"
                     /locus_tag="PK710_00535"
     CDS             complement(8338..9609)
                     /gene="glgC"
                     /locus_tag="PK710_00535"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOT08217.1"
                     /translation="MTPSRHRYPLDVPHVLGMILAGGEGRRLGPLTADRAKAAISFGG
                     RYRLIDIVLSNFVNSGIVRIKILTQYKSASLEEHVARAWRMSAILDNFIETIPAQQRT
                     GKSWFKGSVDAVYQTQHVISDEAPDIVCVFGSDHVYKMDLRQMLAEHLDVGADCTVAA
                     IPIAREKARAFGCVTFDDHGSVTGFVEKPENPPPIPSQPHLSLVSMGNYVFNATALLE
                     ELELDSRNPFSTHDFGRDLIPAMHIAGRKMHVYDFTHNRIPGESEHERGYWLDVGTVD
                     SYWAAHMDLVSIHPHLNLYNPRWPIRTAGSHDPPPKFVFRDEASARVGIATDSLVSGG
                     SIISGGRIHKCVLGRRVRINSFSEVEESILFDEVSVGRYARIRRAIVEKGVQIPPGVE
                     IGLDLESDKRKFHVSAGGVVVVPKNAVFSKG"
     gene            9745..10431
                     /locus_tag="PK710_00540"
     CDS             9745..10431
                     /locus_tag="PK710_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08218.1"
                     /translation="MTFTRSLVAMAAVTQLVILTGCGDSAKPYSPKPAWSGTPVALPD
                     VPTIPARPVKVGDAYTVWGASHHLRSIVHVDDVKNKDISIIGWVVKTNFADAPACAVH
                     PTGKADPDGCNPPVPAFWIADEKGDTKNAMKVLGFASNFAQLYDQIKKDEKAKDGDTP
                     NQDEFLGTPLPMPTPNVDAKVKISGTYGYAYTKATSGIETDPRMGVMTYAKLEYIEKP
                     PEPAKLPGMK"
     gene            10571..11779
                     /locus_tag="PK710_00545"
     CDS             10571..11779
                     /locus_tag="PK710_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOT08219.1"
                     /translation="MPDFLWEARSRTGELRKGVMSADNAATVEQRLRQQQLTPTQVKK
                     KPREFTITIGSGVDSKELVTFTRQFATMIDAGLPLVQCLDILANQQENKIFQAALRDI
                     KSSVEQGATFSDSLRKHPRIFDELYVNLVHAGEVGGILDSILNRLAVYIEKNVKLMRQ
                     IRGALAYPIAVILILIVIVIVMLTFVIPAFERMFADFGNKEALPKLTQMVIWVSRNFV
                     TALPVVIPVTILLITGGIFFYRNPKGKRFVHHALLVIPIMGPVMRKIAVARFTRTLGT
                     LLQSGVPILDSLEVCAKSAGNVVVSSGILFARDRISEGKNMAEPLAESKVFPTMVVQM
                     IAVGEQTGALDQMLNKIADFYEDEVDVAVAALTSLLEPLIMVVVGTVVGTVLIAMYLP
                     IFSLAGAMKE"
     gene            11813..13114
                     /locus_tag="PK710_00550"
     CDS             11813..13114
                     /locus_tag="PK710_00550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT08220.1"
                     /translation="MKIPLGLPYEPSALGPRLATLTLFRLIFLSALLVVLAQWHLRDV
                     HLRAFSVQIALIALAIAFALAAGYALVLRSGKRYRELSWAQIFFDQATWTALVYVTGG
                     AASGASSLYGLTVVTGAVVSGLTGAAIAAVVGYVFYISLSLMMIARWIPVPSDQLDVP
                     YALTLEQAASPLALNLLVITVVTLLSGYLAERLRLTGGKLEEVTERAMQAERLAYLGK
                     VAAGLAHEIRNPLGSITASIELLHDTPGLSQEDRKLCEIIKNESSRLNDLVGDMLDLA
                     RPRKPDFQPTNIIYLLHEVVTLAHRSGRGSQDVQVALVVPDEPSAIVDADPAHFRQLV
                     WNLVRNAVQATSPGSTVTLRLETVGSTAFLLEVEDAGAGIPEEIRAHLFEAFVSTRVH
                     GSGIGLAVVKRITEEHGWTIEAVGNRHGGATFRLSIPRNQP"
     gene            13331..13930
                     /locus_tag="PK710_00555"
     CDS             13331..13930
                     /locus_tag="PK710_00555"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT08221.1"
                     /translation="MNSLRPSFFGALALTFALSFGQLAHAGPAQDFVRAKHTSLTTEL
                     GKAPSPARQKNVEHLMDGFFDFDKLTKDALARHYDDLTESEHNELKALVQALVTKNYR
                     KNIEKTLNYEVAFLGDEAIDGGKGQKVRTRASKRNSGDAPVEITYYLHKKGDAWMVYD
                     VETDGSSMLRNYRNQFGKVIRRDGFGALLAKLQDRLDKT"
     gene            14036..16351
                     /locus_tag="PK710_00560"
     CDS             14036..16351
                     /locus_tag="PK710_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08222.1"
                     /translation="MSKHPAKVSRKSAPSSEPKVLMQLSACRGGLALELAEPFTVGPL
                     TVTRLVSSLQGLSFPIDLTGGVGRFRHRRGVLDHLVVESQLAQLAMALQPRARAVFDS
                     PKLRLSLLPTALGLSVGVCDEDRALSFSAHFTSDGPTLQFVVSDARAVGFGFHSHALA
                     VRLARALTGCTQSQGSLISLERGIAHLVREVLLDAGARLPSCQQLQYAWDETLDGIRL
                     TASSAAPEACPAAEVLRAIELATIARQGDEALVAGNFDDARAAYVTALESAPRHPDLA
                     LRIAEIDLLAGYAPESALASLVETMPALDGGLIGAELLAALGDRQASVLALRRAADRE
                     PFAPMAARMLGRAAAITEERSDRLSLLDEALARCVGCESIRWQRAITRLEAGCFSDAI
                     ADLGHLEAAAQGAEKRYQVCLRAGQHLLQARLALDARTFFEKALRYAPRSAQASSGLA
                     RAFYALGDGRRAVRLLTRATELSGSSSPPPTTLLELATAIVEFTRDYPAAIYHVRSIP
                     FGLPQTVAARALEGRWRNQLGDIVGASHAFSQAREAAAQLPRDTIAEQSQWLIEAAQF
                     ELDIRNDPRVAKRHVELALQADPNNPHVQQVFRRAVREEQQAVATRMDPVASPESHDG
                     AAASDSPLVIADEPITKSAVSSTNLFDPASTSGEIYDEGPLDANEEAQIEVLEDRVLA
                     NPNDQETIRQLSELLDRGKQYPRLLALVSARLDETSEPSYRAELMAMRIHALQQLMAA
                     CRAQGNEGEAELYEAALADATESSSDDSLEG"
     gene            complement(16314..17222)
                     /locus_tag="PK710_00565"
     CDS             complement(16314..17222)
                     /locus_tag="PK710_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOT08223.1"
                     /translation="MTESEIFAVMIDGQVVAQDQARISVFDRGFLYGDSIYEVMRTYD
                     GKIFALREHIDRLFRSAEQVFIDIPVSKDRLSQEILHAASLLELPNLLIRVHITRGIG
                     PYGLDPTGARQPTRVMIVVPLLVPSEDQYRDGIAAALFQSQRTTDNTSAAGAKVANYL
                     VSLLALRQAHAVGAKEAIIVDGRGRVLEGASSNVFIVRSGVIRTPPVNEGILSGITRS
                     YLLKAAAHEGLELRETSLTQTELLDADEVFVSSTTREVLPVTRIDDRTIGTGKVGPIT
                     RAIHRAFRAIGGLPAILPGNRPNSTQ"
     gene            complement(17219..17881)
                     /locus_tag="PK710_00570"
     CDS             complement(17219..17881)
                     /locus_tag="PK710_00570"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HOT08224.1"
                     /translation="MLSMLVGLALGLVARAWLLTVRLRVFQHPLLNDSSQPRVLALWH
                     GQLLLLLAHRRCRRTIALVSHSPDGERLTWAMRWFGVFSMRGSSSKGGRQGLQAIVQR
                     VQEGWDAALAVDGPRGPRQVARPGALAAAYRAHAWVVPYAAACSHGFCLASWDRFIIP
                     RPFSRMVIVLGKPITNPSAINERDLACAIDHTTQRAHDLLLSWSATKSAPCTLSHFHA
                     NP"
     gene            complement(17886..29096)
                     /locus_tag="PK710_00575"
     CDS             complement(17886..29096)
                     /locus_tag="PK710_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT08225.1"
                     /translation="MSSQKIRNALGLLQDDEDNEAAWLELQDALTSPDVGLSDEELID
                     LLGAARREHETRREWSAVANLLEYEIAQCQGSSKEVARLAELARVLEDELLDDKRAVD
                     VYKRWLDLRPDDPTATEAMARVEDQRAVWEELAERKLEEAKGVEDSSIRSSMLSWVAE
                     AKFRFGREQLDLDELMALLEQAIELDPRNRRACMLLERVYRQVERWNDACRILEILAT
                     ESPVREERFAAWLRLARVVVRKLGAEARGVAAYERALDLVPGYPEAMNFLSDYFSREE
                     QWDHLVALYEDHLRSGAFKSGQDLGILLQIAMVHWRMRGEPDLAEPYFDKVRRTEPAH
                     LGMLSFYRTWCEQRKDTSRLITILTDAQRSLPDGDQKAQLAAEIAQLAENQEDVHKAI
                     EQYKALLRQDPGNREARGALKRLYRQTSAWTSLVDVLRQDLDAIAADDAQGRLEVLRE
                     IAAVYRDHIKSDSALVTVLGQILALDDSDVAAVRELCRVYESLQRWRDLLVHQQKLAE
                     LSDDPQEKLSLLRSAGKRWMEQFQNVQNATDVFERVFALDPTDEEARQRLRDLYQRRR
                     AWPQLFGLLGKEVEYADEETKVRLLTEMAKLAAERLDRAADAIALYKQILEIDPHAPG
                     VMDALEKQGERDKDYETVAQVLERRVQTAEDENARIAVLQKLGGVYAERLNDPRRAAS
                     AWRRVLEIRPDHSRAMRVLRDAYLAAEDFDAITELYGQNDDWEGLAEVLSSAADRTTV
                     TESKVDLSFRVAEVFESKLQAPERAFRAYERVLSVRPDDLRAANALVPIYEKDERWSR
                     LPALYEILLARSEDPERQLELLRKLADVTGNRLSDKEAALTYARRAFDLAPDAEGALE
                     LLEGYARAAALWEPFVEALEKRIANDPDMPADEIRALQLRLANVYASDLGRMEDAIKT
                     YRQIVAEDPSDIDILKTLDRILRAAARQDDLRWLFELRIERADDSSKIALLTEWATLE
                     EDAFSSPDGAIALYRRTLQLDPSNTQALTSLPRLLLAEGNAAAAAQIVETHRDQKQGA
                     ERGELELVLAELYVQRLQRPLDALDAVQRALEHDADSARAIAVLDALVGTEETRARAA
                     SLLVGVHADLGDPTREADALALLVDATTDSQERLQLLLRLIDVYELKMRDASTAFDVV
                     LKAVTEFPEQVSLWDRARDLSIVVERPGELAQVYRSVLDTQLSLPDDIEVELCQRAAK
                     LCREHIGDQDAAIPYLERVLARQPANEEAFKDLKEILMSRERWQDLEALYARAITGTT
                     DGARRIEFLAEVAMICEDIIEDPSKSIDYYERILDIDPLHGTATMALEKLYASEKRYD
                     KLVSLLEGRLNRAGESDLIAIKLRLGKLHLDNLGNPVNAMPHVEDVLHLRPSEQEARD
                     LAERLLTVPALRARAARVLEEVYENRDEIRDLVRVLDIRLEVTEDESERRELLRRMAA
                     LRDERLADDQGAFEALTKLVPLDPIDGHARSRLIDIGRRIGEHETAAKVLTQAAVLSL
                     TPDVQGAILMDVASIYRDHLSDVERAEAVYRRVLAIDPADPNLVLPAGRALEEIYAGL
                     ENHVALVEVLRTEVKLEDDAQRRAEIYSRLGEICENILEDWPGAIDAWRSRLEDDPVD
                     PAALVALERLFEKVQAWRDLVSVLRTRHDGAEDADERRRLMVRIAETLTNQLQDVSEA
                     TLAWRAIVEDYGPDPSTLEALETLYEKAERWTDLSDCIDTHLNLADEPADRLALLVRL
                     GDVRREHLQDLDGALDAFRQALTVDPSHKPSREALERLLTEEGAKREAAQVLHPLYEA
                     DGDNEKLLRVVEIEADTTLNPDERLSLLQQAVRVAEGPLDDASRAFGFAVRGTKEAAG
                     SEEITIWLEIVERLATTTRRFSDLVTLLREVVTDILDGDVQLDVTLRIAELARTELAD
                     RELAREHYIRALDIRGDDRRALVALESLYEEIGDATALLDVIRRRVDVAENDEERKQL
                     LFRQAKLCSDVLEDSEGAIAVFETVLDIDLDPAAVEALETLYSQAQRPSDLISLYERQ
                     LEQSTEGKAELRVKIAQVAEGKLGDTMRAFDELGEALAEDSQNEAAIAELERLLDSAQ
                     DPENRARAAEMLEPFYLGRADWKKVKRTIAARLDACPDTDKRRELLRRLAVMQEEQEE
                     DFSAALETTAKLLHEDITDRDTWKQLESQARVAGAERRLAEIFAAELEHVVSDDEATA
                     SLARRTGELFASLGDTEKSLLHYRRALAFEPDSTVLFDAIDKLLIDAQRAKDRVELYQ
                     GALDHRFEPEDRIRILHTIAELERTALQEPDKAIETYCAALDVDENDSVSLDALTELY
                     RQRERFADLAELYARRAENDSDVDRACEYRLALARLYRDEMNDADSAVDQLEEIVRQV
                     PSNNLAIAELEALLAREDQKARVVDILLPLYEGADDWRKQIYLNAQRFDLSTDELERV
                     AILRETARLWELRGKDNQRAFDAFRAAFEIDAEDMDIRQELERLAGTLGAWDELAEAY
                     EEGIEAAEHSGKRDLLSALATIHDEKRDDPRRALDAYSRLHQLDETDAEPLEKMDVLS
                     MLLADWQVLVSVLMKKAELIGSDEDRALAWRRVGECKRDMLEDAHGAIEAFEHALELE
                     PASASTIDALIDLLEARNAGDKLVELYQRRVELAEGEAEEDLKYDMLLKAANRYQKDL
                     EDNQAAIDVLRQALEVRGADKNVLSKLETLFRVEKMWPELLETLRQEATIAEDKKERA
                     SLRREIGKLLASEMAEPTEALEAFRMVLEEAPEDRDTIEAVREIGEGFEDLRLDAADI
                     LEPVLRNTSQFDMLVTVMEMRLLAQHDVAERALTLRSIATVLHDSLHNTERALEALLR
                     ALAETPEDTGLHDDIERMAEAASNGFQQYADVLEERAGKTFDAALATDLWKRLGRICE
                     QKLENDKRAVEALSRALDQAGDEPELLASLDRLHSKLGNTRELNEILDRRVHVETTPE
                     GQADLYHRIAVLQLESFDDKMTALENLRQALDHVPTHEKACDTLEKLTEDKNLFEDTA
                     AALEGVYRARGDFSRLASLYEKRVSFAEDPSERLRLRLDLAGVLEEQAEDPKRAQRVI
                     EAALEDDVCDSDVLAKLEQLAGANQQWTEACKAFHGALSKADGLDPMTLRDLYVRLAD
                     WYRIRLADGKSAESAMQQALHVDPDNLELLQAIEELQREPGRERDLIATLRRRAILDL
                     DTEKRRELFREAKTLAHAVLADDELTEQVLRQLIELDDGYLWPYEELTQLREKKEDFA
                     EVVELLLKRAELTAHGPDSLQLRHQAAEVLSSQLQEPERALKIYEEVFEADPMDSKAA
                     SALRKLYADQKRFKDLSELLGRLIDVASDASERTKLRLELVDLLVSRFDQVDEAVFVL
                     LSVLDEEPGQTDAVVVLSQLYERTERIRDLADLLDTQIKLAKENGQAEAELTFTVRLG
                     EVYETRLGDKQKAIDTFGAVLERDPSHKQALEALGRLYEATGDKQKAVETLERLLALL
                     EGDEAVTLALRLAGIFDLMEDESGARRVLERGLAANPASAEVREMLRKLYERTGAWAE
                     VAAMLAQDAENASDPADKVKLLQRAADIHMTKRNDAATAAKLLDQASELAPDDRELLL
                     SLCDAYSASGRGKDAISALEKIVESYGGKRVKELAAIHHRLAKAHVSEGNKEQGLAEL
                     DQAFRITPGNLAILVDLGRLALDLGDLDRAQKTFRALLLQRLDDKAPITKAEVFYYLG
                     EISHRQGETQRAIQMLERALENDASFEKAKELYEKLKA"
     gene            29597..30280
                     /locus_tag="PK710_00580"
     CDS             29597..30280
                     /locus_tag="PK710_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOT08226.1"
                     /translation="MHSLQKVAIILCALIATLVLIFTIGTTARAAEPPTADQVASRVQ
                     AFYNQTRTFQARFQQDYTVKAFNKRKRSTGQVCFERPGKMSWKYDNPNGNRVVSNGAT
                     LKVYEKDNKQMFEQPINDSQYPAALAFLMGKGDLKKTFTLRLLDAKRMNFEGGYVLEG
                     TPKSPTPAYQKVLMYVDASTAQVRRMLILDAQGNRNMFTFDNPVVNTSIPSGEFNFDP
                     PKGTTIVHP"
     gene            complement(30297..30794)
                     /gene="ruvC"
                     /locus_tag="PK710_00585"
     CDS             complement(30297..30794)
                     /gene="ruvC"
                     /locus_tag="PK710_00585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOT08227.1"
                     /translation="MRVIGIDPGTRHLGWGIVRVQGTRLSAEDYGIVDLDGRLSIAER
                     LLQIDRALGEILRVHEPEVAAVESLFFAKDAQAASKLGHARGVILLLLAKAAIPIHEV
                     EPARVKRAVTGRGRADKRQVALMVKAVLGLRELPGVDATDALAVAITHAQQVFFERAV
                     HRGGR"
     gene            complement(30795..>31347)
                     /locus_tag="PK710_00590"
     CDS             complement(30795..>31347)
                     /locus_tag="PK710_00590"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOT08228.1"
                     /translation="LIEVDGEGPACERDMERTGEACVAVGALDVLVAQAGTQSARLWA
                     ARRELSPAVRKLAKYKLAEDVVVPVSQIPALLEKTEALAERYKVRILTYGHAGDGNMH
                     VNFLWDDPADLPRVLGAIENLFRDVIALRGTISGEHGIGVLKAPYLGLEQSHELVSLQ
                     ERVKKVFDPKGLLNPGKIFPLRV"
BASE COUNT         6622 a         9032 c         8856 g         6837 t
ORIGIN      
        1 gccggcgttt ttgtcgccat ccagaccgac cattggatcg ccatgccgtt tgcctcgctt
       61 ttcgcctttg gatatggcta tgtcgccgta ttggtgatgc gagaacagag tgccagacgt
      121 cgtgccgcgg cgcgtttgga aatgcagcgg gcgacggtcg cgggttaatc agatatcaag
      181 gggctggact cttagcccgt gcgaggtagg atctcaggct gtaccggcgg cgctcattca
      241 cgcaccctgg gagggttcat gacgaaggct gatattgctc atatcattta cacgagaatt
      301 ggtggcttca gcaaaaagga gtcgacagac ctcgttgatt tggttttcga aaccatgaag
      361 gaaaccctcg gtcgtggtga taagatcaag atttccggat tcggaaactt cgtgcttcgc
      421 gacaaacgaa agcgtcaggg ccgcaaccca caaacaggcg agcccatcac catcaccgaa
      481 cgtcgcgtgc tgagtttcaa acccagccaa atcctgaagc aaacgctcaa tgacgcccca
      541 accgcctcca gcaccgaagg ctcatcaccg aatcgtcatc cttgaggcat ctaggttccg
      601 accatgtcct cggacggaac gcagattcgc aagttgtatt atcgcatcgg ggaagtagcc
      661 agactgctgg gcgtcgaaac ctccgctatc cgccactggg agtcggaatt cccaagcctt
      721 cgacctcggc gcaccaaaac cggacagcgc atgtattccc agcatgaact cgagcgattg
      781 aaagagatca aacggcttcg tttcgacctc ggctatacca tccgcggcgc tcgcatggcg
      841 ctgcgaaaca aagggctcga agcaagagaa ccccaagacc ctgtcgtcat cgacaacgaa
      901 cagctccgag acacgttgct cgacttgcat cagcaaatca cttcgttcat cgatgccttg
      961 gaacgggagc gaaatcaggc atgagcgctc ggactgcact cgtccacggc acaatggcct
     1021 cattgctcgt tgcttccgcc acggtccttc ccgccctggc tttcgcacaa caagatgcca
     1081 gcgcggacgc tttcgacgca gaggttgcca tggacacggc atccgatgcg ttggaatccg
     1141 cggatgcaga acaagagccc accgctgcca cgctcggtcc cgaaacgtcc ttggtcattt
     1201 ccgaaggcgt gaatctgcgc ttttcccccc ctgaaggctg gatgagcgtc gaatcaaaac
     1261 ccgtcgatat tccagacctc gataaaatcc cgggagcaca agcacttttc aaacatacct
     1321 ggacggctcc gcatcgtcgc aaagccatgg cgcctcggat gtcggtcgta tgcgcaaccg
     1381 ctcccagcga tagctggacc cgcggcctag agtctcgcgt tttcgaacac ctcacgagcg
     1441 tagcccacaa cgaactcagc gaatggatga gtgtcgattc cttgcaatca ggccccatcg
     1501 actcaacttc gccacttttc tttcagtctt tcgaagccaa aggccgcgcc ggaggccaac
     1561 gaaaaaccgg tgcattgcga gtcctcgaag aagacgccat cgacaataaa cgtgccccgt
     1621 tcgtcggcgt gggacgccat acccttggtt ttctcgctaa tccggaccgc gtcttcgtct
     1681 gctcgatggc atgcatagaa cccgcctggc gagagacggc catttgcccc acctccgtcg
     1741 cctcgctgca tatcgatggc gctctcgccc ccgaaccttc ttcctccacc ctcgggcgtt
     1801 tggccatcgg tatccaacga cgtccctccg ccctggtcgg catggctgcg ggcttgctgc
     1861 tcgcgtttgt gggaactctg gccgtattgc gcggctcgtt gctccgacca gcatccaagt
     1921 catgaatcgt ggattcggat ggcccacgcc aatcgcaaga cttgtttcac gacattggga
     1981 acacgacgca ccaagttgat ggaaggtcta cgtttttcca cgacgcgaac agggatttcg
     2041 cgaatggaaa gcccttttcg atacgcacga atcacgaact cgctcgcaaa aacgtccttg
     2101 tccacgacac aagcatggac cacaggcagt agacgttcgc gatgaaacgc tttcaaacca
     2161 tgcgtatcgg ttccacgaaa ccccagcgtg gtgcgcaaca gcccgttgta cacacggctc
     2221 gccatgttac gagcccacgg ccgttcatcg ctcgaacccg acaagagttt cgaaccaatc
     2281 accaggtcca caccaccatg ctgcaacaac gccaccgatg attcgtggaa gaccgtatcg
     2341 caaagatcga tctcatcaca tacgacgaac gtgcctttgg cctcgagaat ccctcgtcgc
     2401 aaagccagtc catagtttgg ctcacccagt gaaaaccatc gtatttcctc atacttagca
     2461 gcaagcggag ccagtatggc cacggtggcg tcggtcgatc cattttccgc gatgatgatt
     2521 tcgaacgtcc acggggcttc tttgagtttt tcacgaaggt cgacgatggc cgcgcgaagg
     2581 atagcttctt cgttgaacac cggaatgacg atcgaaacgg taggggtcgt ggtcatggca
     2641 acgatttcct ggcccaatga gccgcggctt ggccgaacag cagagcatct tccatggacg
     2701 aataattcca tcccccatag cgcccggtag agagaatgtc ttggtctttc agaaagccat
     2761 gcacgctatc gaccgaggca taataggcgt gatcgaagat gacgtaggcg tgttcgatgg
     2821 ttcggtgttc cgcgaacagc acttggttgg gctggtcgat gaatcccatt tccaccaagc
     2881 cggcagccac atccggcacg agagactcga ggatgggagg ctgtcgatcc gccagttcga
     2941 tgtacataca agctttgccc tcgggggcca tttctttcga gaagttggaa tatacgccca
     3001 cccgataaaa cggatatcgc tgttcaggaa catagaccca gtggaaagga tgggccgagg
     3061 gcacatcgag agcgacatcg aggtaatgaa gagagctaca ccgaagtttg gaggccgctt
     3121 gccgaatggg ctcagcaatt ccctcggtgc gcgcgagcag atggtccagg ggaatcgttg
     3181 aaatcagcac gtcgtaatcg atctgctccc catcttgcag cgtgagccgt tttcgttgtg
     3241 ggtcgagggc cacgagttct ttgccgcatt ctacagggcc gacagctttc gccagcgcgc
     3301 tcgtcagggc tccaattccc tttcggggat acagaaaaga ggcattgtat cccatctccg
     3361 gtcccgccag tccaaccgcc cccgcaatca cttcctccaa cgtgggcaga ggaacgaaac
     3421 ggctgcacca ttccgccgtg atctcgcggg gatgcacgcc ccagagccga ctattgtatg
     3481 gaatcatgaa gtgtttgctg aagccatcac cgaaatgctg aagacaatac tgttcgaaat
     3541 ccgtgggagc ctgcgcatgc ggctggcgca gcgcatgaac gaaaccgacg atgcattcgt
     3601 acgccacctt ggcaggcagg cccaacgtat tggcctggaa cggatagcga gtatatgccc
     3661 cgtgggacca aatcatggac cgtcgctcga tcgagagcca ttcgttgccg agccaggcgg
     3721 tggcaagcga gcgcatggcg ggatcgcgaa gatgcagaag atggccggtg cgatcgaagc
     3781 gaaaaccaga ttgctctacg gtgatggcga gtccaccggg ctggggggcg cgttcgagca
     3841 gccggtgacc gatgcccatg gactgcaggt ggtaggaggc ggacatcccc gtcaaaccgg
     3901 ctcccaacac gaccaccgat ggttgtttgc tcgacatggg agacggcact ttaatcggtc
     3961 ttgggcaatg tgcaatgccg acaggatcat gtgcctgaca cgaaagtcgc gcccttgcct
     4021 tcgtcaacct ccgcaattcc cgatctttcc cccttggcac atagtttgaa caatgtccga
     4081 tgttctggca ttcctatcgt cggattcgtc atgccatgct cctgggctat ccacgatttc
     4141 ggtacgattc ttcaccttgg agggaagagt tgaatagtct ttgcagtaga tcgtcataat
     4201 aggactgctg gtacaagacc tcatggaggg aacgagaatg aacatgcgat tggctttggt
     4261 tccgttgtgc ctactttctg cggtgagcgt catgggttgc accgcaccgg aagaagactc
     4321 ctgcaacatc aaaactggtg gaatttatgt cgagtacgag gtgtttgaag aaggcaacca
     4381 ggctcgcgcc accgctacgt tctgggttgg cgacaaaccc ggtgggacgt acctttccct
     4441 tggaagttgt ggggataaca tcaccgtcaa cggccagggc atgtcgaaaa aaaccgacaa
     4501 cgtcaatcac gactattacg aggccaagct tggcaaggct gactcgtacg aattcgtgtt
     4561 tacccgggaa gacgaagacc cgtattcgag cagcgtgagc cccagaaagc cggtcaacat
     4621 cacggcaccg accagtggca cgtcaatctc ccgcgcggac gctttcgaaa tcacctggga
     4681 gaataacgac agcgggaaaa tcgagctgct catcgaaggc gattgcatcg aagacttccc
     4741 gagcgtcaac ggagatgacg tggcggatac gggcagctat tcggtcaacg ccgacggcat
     4801 caagccgttc atgaaggacg atgcggacaa gacatgctcc gcttccctca gcctaacccg
     4861 caaaaacagc ggaagtttga gcccctcgct caaaggaacc atcaaaggct actccatggg
     4921 acacacaagc ttcacgtcga ctccctgaga ttcttcccgg ctaaggtgcc acgcgcccag
     4981 ggcggcaagg acagcggttt ccgttcgaag aatcaggccg cccaacgagc caagtataaa
     5041 tccagatgct tccgcctgac gggcttcttc gttttcgatt cccccttcgg gaccgatgat
     5101 gagggcaacg ccgtttcggg ccgttcgaat ccactttccc acgggctccg tggctttttc
     5161 ccacaaacac aggcgaacga gcgagtcggg aacccgcgat aacgattgct ccaacgtgca
     5221 aacatcgtga aggatgggaa tatttccccg accgcattga cgtgctccct gcaccaggat
     5281 ttttcgccat cgaaccatgc gcttggcgtc gggccggtgg gtcgaagagg ccgaccgaga
     5341 gagcatcaac ggcacgatgt cggtgactcc cagagcggta gcctcgcgaa gggcccatgc
     5401 aaaggattcc gttttgcata agccgagcag caaccaagcg ggcgtggaag gaacacaaga
     5461 ggcggttttc acgtcgctca attcgcaaac gacgcggtcc cgatgtatgg aaaccaccgt
     5521 tgcctcggct tcggtagcct gttccgggtc aaatacttgc aacctatccc cgggctgaag
     5581 acgaagcaca tggcggatat aatgagcagc ctcctgatcg agccgtcgcg ctccgggttc
     5641 gatccccgcc aagggaactc gaacgatgct caacgaacgc tctccaactc gatggcgacc
     5701 caatcggatt cagccatcgt ttggcgcaaa cgaagcgcgc catacgccgc aatcacctca
     5761 tccacctgcg ttcgaaggat accgctcaac acgacaattc ctgccggggc aagacgtcga
     5821 acgataggtt ccgccatggg gatgagcacc ctcgcctcga tattcgctac gatgaaagga
     5881 taagaggatt cgatcgtggc gacatccgag gtgtcggcgg ttatccgatg ggccaaatca
     5941 ttgcggaggg cgttttcgag ggtgacggcg acggcatcag ggtcattgtc gacggcgcgc
     6001 gcgtgtttgg ctcctagcac gagggcgcat aaggaaagca cgccactgcc acaacccacg
     6061 tcgagaatgg tgcagtcggt gagtcggggg gcgagattat ccatcgattg ggcgacgagg
     6121 cgggtggtcg ggtgcaatcc cgtgccaaaa gctcttcctg gatccatctc cagccaatgc
     6181 acgccaggcc gcggctccgc gggagcccag ggcggacgaa tggcaatatg gggggtgagc
     6241 aaaaagggtt ggaaatgttc tttccagcta tcacgccagg catctcccag cacatcgtcg
     6301 cgccgtgggt tccattcggc ggcgagatga gcgatggctt gcgtggcttc gtcgtcggag
     6361 gaaaagctcg ccaataaggt gacggtaccg tccacccgcg cttgttccca cgtggtatgg
     6421 tctcggattt cgacgccggt ggcgtgcaag tcgaaaaggc cgtagctgat ttcatcggcc
     6481 agcggagcgg gaacatccag ggccacgcgg gggaatcgcg gaggagaaga agcattcatg
     6541 cggtttagtt tccaatgaca atcacgacac gactggtcgg ggcgggttgc tggggaggcg
     6601 gtttttggga atcggttttg gtgtcgctaa gcaggatggc gcctgtgacg ccgcccgcga
     6661 cgacagcagc agtggctacc cagaaccatg ggcttgcgaa cagggagccg cccttttcgg
     6721 gctccgggat ggcgattctg agcggtgccg ccgcatctcc ccgggatgca atcggtaggt
     6781 gggcttcgtc gagggcctcg atatagtatt ccaccagcgg tggggtgagc atcgggccag
     6841 ggatggaagt gcggaacacg ccctgggcaa ggcgggcttc gaggtgtcgg tacttgcctt
     6901 tcgagccgga gcgaacgtga acgacgatgg aagcgacgcg atggtttggg tctgcgattt
     6961 ggccggtgac ggggacgggt tttcctcgtt cccattcggc gggggaggca tgtttgataa
     7021 cgggggccgc gagcgcggca ctgccacgct ccagcccggg gcgtccttct gcttcccaac
     7081 gctccctcac ctctttgaaa aaggaacgga atctcggcga ttcggtgtct gcgagggaaa
     7141 attgtggatc caacacatac aatccacgca ccgcggcctc ggcttcgtcc atctgactga
     7201 gcaccagata gttgaaagcg aggtatcggt acacgtcgat acggtcggat tgggaagtcg
     7261 ttgggcgaag caacgcggcc gacagggttt ggatggattc ttcgtagcgc tggtcttcga
     7321 acaactgcac cccggaacga atcaaatcag tttgggaagc aggtcgggag gattgtccga
     7381 aggcaggaca aggagatatc gacagcaggg tcgaggacag gaaaaggatg agtccgacga
     7441 tcgagatgcg atgcgacagc ggtttcgtcg acataaggga accgtaccac gtccgggggc
     7501 ggtctttcat cttggagttc gtcgaagaga gcatcggcga gagcattgaa tccgtcgaag
     7561 acgtcagcgg ccataatttt ttcgtacggg gtagtaagca cgccgtgcgg aaacggagac
     7621 cagcaactcc tgtctttcct cgatacttgg gatcgtttga ccgggaccga ggaccaaagc
     7681 ggccaaaaag ccgccgtaga cgcatcccag gtcgatgccg gtcgcccagg ggtggagttg
     7741 gggatgcatc agggcgttat ggccgaaaac gatatgcggc ggcccgtggt accaacgtgc
     7801 ccagggttct cccccccggg tgatcgaagc ctgtcccctc tgcgtaattg cgcgaattgt
     7861 aagtagaatt ccagtatccg tcgcctcgat ggggactcct ggtttcacac ccgcgtgaac
     7921 gatacgcagg tcgtgttctg gcaggtcatg ccatagcggc atggcttcga gccaggccca
     7981 atcttgttcg gatagggagt gaacgagctg caggtgtccg gggcccaggc gcacgagcgg
     8041 gcctccttct cgttgcgact gaacgaaact cagtagtttt tgctcgtgat ttcctcggac
     8101 ggcgacagct ccgagttgtt gagccaaggc gaggacaccc agggtatcag gcccccttcc
     8161 taccaggtcc cctacgaaat acaggtggtc atcgtcggaa aaccccacgc gagcgagcaa
     8221 ttcttgaagc tcggcgcaac aaccgtggac gtctccgacg atgacggtac gggacatagt
     8281 gccaacacct agtcggccct tatgaaccac gcaacccaaa ccgggaccgc tgctcggcta
     8341 tccttttgaa aagactgcat tcttggggac aacgacgaca ccgccggcgc ttacgtgaaa
     8401 ttttcgctta tcactctcga gatcgagacc aatttccacg ccggggggga tttggacgcc
     8461 tttttcgacg atcgctcttc ggatgcgggc gtagcgtccg acggaaactt catcgaaaag
     8521 gatggattct tcaacttcgc tgaaggagtt gatgcgaacg cggcgaccga gcacgcattt
     8581 gtggatgcga cctccggaga tgatgcttcc gccggacacg aggctatcgg tagcgatgcc
     8641 cacgcgagcg ctggcttcgt cgcgaaaaac gaatttgggg ggagggtcgt gggaacctgc
     8701 ggtacggatg ggccagcggg gattgtagag gttgaggtga gggtggatgg aaacgaggtc
     8761 catatgcgcg gcccagtacg agtcgaccgt accgacatcg agccaatatc cgcgttcgtg
     8821 ttcggattcg ccggggatgc gattatgggt gaagtcgtag acatgcattt ttcgtccggc
     8881 gatatgcatg gcggggatga ggtcacggcc gaagtcatgg gtggaaaacg gatttcgcga
     8941 atcgagttcg agttcttcga gcagggcggt ggcattgaag acgtagtttc ccatggaaac
     9001 caacgataga tggggttggg aagggatggg gggagggttt tctggttttt cgacgaagcc
     9061 tgtcaccgag ccgtggtcat cgaaggtgac gcaaccgaaa gcgcgagctt tttcccgtgc
     9121 gatggggatg gcggcgacgg tgcaatcggc accgacatcg aggtgttcgg cgagcatttg
     9181 tcgcaggtcc attttgtaga cgtggtcgct tccgaacacg caaacgatat ccggggcttc
     9241 gtcggagatg acgtgttgcg tttggtacac ggcgtcgacg gagcctttga accagctttt
     9301 tccggtgcgt tgttgagcgg ggatggtttc gatgaagtta tcgaggatgg cggacattcg
     9361 ccaggcgcgg gcgacgtgtt cttcgaggct tgcgcttttg tattgggtga ggattttgat
     9421 acgaacgatg cccgagttca cgaagttgga cagcacgata tcgatgaggc gatatcggcc
     9481 accgaaggag atggcggctt tggcgcggtc ggccgtcagc gggccgaggc gtcgtccttc
     9541 tccaccggcc aggatcatac cgagtacgtg agggacatcg agcgggtagc gatggcggga
     9601 cggcgtcatg gttggtcgat gatatccttg cttgtctccg aacaatagcg ggcatgcgtt
     9661 gctagccctt gcccaacggc ggcagtccgt gatacgcatg cagcgctcgt tgtgtgaaca
     9721 tcaatcaagg agtctggagg gcgcatgacc ttcactcgtt cgcttgtcgc gatggcggct
     9781 gtcacgcagc tcgtgattct caccggttgc ggcgatagcg ctaagcccta ttccccgaag
     9841 ccagcttgga gcggtactcc ggtggctctt cccgacgtcc ccacgatccc ggctcgtccg
     9901 gtcaaagtgg gggacgccta tacggtttgg ggcgcatcgc accaccttcg cagcatcgtc
     9961 cacgtagatg atgtaaaaaa taaagacatt tctattatcg gatgggttgt caagacaaat
    10021 tttgcggatg cacctgcttg tgcggtacat ccgacgggca aggcggatcc cgatggttgc
    10081 aaccctcccg ttccggcatt ttggattgcg gacgagaagg gggataccaa aaacgcgatg
    10141 aaagtgcttg gttttgcctc taatttcgcc cagctttacg atcagatcaa aaaagatgaa
    10201 aaggcgaaag atggcgatac gccgaaccaa gacgagtttt tgggtacgcc cttgccgatg
    10261 cctacgccga acgtagatgc aaaagtcaag atatccggga cgtatggcta cgcttatacg
    10321 aaggcgacct ctggcatcga aacggatccg aggatgggcg tgatgacata cgcgaaactg
    10381 gagtatattg aaaagccacc agagccggca aaactcccgg gaatgaagta acgtcgggtc
    10441 gacgcgacgg tttcgcaact gccttattct tcttgtcagt gtcgcattat atgctcggtc
    10501 tttggccgaa tacgtcaaga accgttaagc tgcggtaact caaccaaagg gtcccaagag
    10561 gtttccgagc atgcccgatt tcctatggga agcacgatcc cgcacggggg aactccgcaa
    10621 gggagtgatg agtgcggata atgctgccac ggtcgaacaa cgactacggc agcaacaact
    10681 cacgccaacg caagtcaaga aaaagccaag ggagttcacc atcaccattg gctcgggggt
    10741 ggactccaag gagcttgtca ccttcacccg gcagtttgcc acgatgatcg acgctggctt
    10801 gccgctggtt caatgcctcg acattctcgc caaccaacaa gaaaacaaga tctttcaagc
    10861 ggccctgcga gatatcaagt cctcggtgga acaaggagcc acgttctctg actcgcttcg
    10921 caagcaccca agaatcttcg atgagcttta cgtcaacctg gtgcatgcgg gcgaggtcgg
    10981 cggcatcctc gatagcattc tcaatcgtct ggcggtgtac atcgagaaaa acgtcaagct
    11041 catgcgtcaa atccgtggcg ctctggcgta tcccatcgcg gtcatcctca tcctcatcgt
    11101 catcgtcatc gtcatgctca cgttcgtcat cccggcgttc gagagaatgt tcgctgattt
    11161 tggcaacaag gaagcgttgc ccaaattgac ccaaatggtc atttgggtgt cacgcaactt
    11221 cgtcaccgcc ctgcccgtcg tcattcccgt caccatcctg ctcatcacgg ggggtatctt
    11281 cttttaccgc aatccaaagg ggaaacgctt cgtgcatcac gcattgctgg tcatacccat
    11341 catgggacct gtcatgcgca agatcgcggt cgcgcgattt acccgaacgc tcggcacgtt
    11401 gcttcagtcc ggtgttccca ttctcgattc gctcgaagtc tgcgccaaaa gcgccggcaa
    11461 tgtcgttgtg tcctccggca ttcttttcgc gcgagatcga atctcggaag gcaagaacat
    11521 ggccgagcct ttggccgaat ccaaagtgtt tcccaccatg gtcgtccaga tgatcgccgt
    11581 cggcgaacag accggcgcgc tagatcaaat gctcaataaa atcgctgact tctatgaaga
    11641 cgaggtcgat gtcgcggtcg ctgccctcac ctctttgctc gaacctttga tcatggtggt
    11701 cgtcggcacc gtggtcggta cggtgctcat cgccatgtat cttccgatct tctcgctcgc
    11761 cggtgcgatg aaagagtaag gtccgggtga ttctccccct gttcgatcgc ccatgaaaat
    11821 tcctctcggc ctaccgtatg agccgtcggc tcttggtccc cggctcgcga ccttgaccct
    11881 atttcggctg atttttcttt ctgctctgtt agtagtcctt gcgcaatggc atttgcgaga
    11941 cgttcatctc cgggcgttct ccgtgcagat tgctctgatc gcacttgcca tcgcttttgc
    12001 gctggctgcc ggatacgcgc tcgtattgcg atcgggaaag cgttatcgcg agctatcttg
    12061 ggcgcagatt tttttcgatc aggcgacatg gacagcgctg gtctacgtca cgggtggtgc
    12121 cgcgagcgga gcttcctcat tatacgggct gaccgtagtc accggcgcgg tggtcagtgg
    12181 attgacagga gctgccattg ccgcggtcgt tggctacgtt ttctatatct ccctcagttt
    12241 gatgatgatt gcgcgctgga ttcccgtccc atccgaccaa ctggatgttc cctacgcact
    12301 cacgctcgag caagccgctt cacctctcgc gctcaacctt ctcgtgatca cggtcgtgac
    12361 attgctgtca ggatacctgg cggaaaggct tcgtttgacg ggagggaaac tggaggaagt
    12421 cacagaaaga gcgatgcaag ccgagcgtct tgcgtacttg ggcaaggttg ccgccggtct
    12481 tgctcacgaa attcgcaatc cccttggctc gatcaccgca tcgatcgagt tattgcacga
    12541 caccccagga ttgtcgcaag aggatcgaaa gctgtgcgag atcatcaaaa acgaatcctc
    12601 gcggctcaac gatctcgtgg gggatatgct cgatctggcg cgtccgcgta aaccagattt
    12661 tcagcccacg aatatcatct atctgctcca cgaggtggtg actcttgcgc atcgctccgg
    12721 gcgaggcagc caggatgtgc aggtcgcgct cgtcgttccc gacgagccat cggcaatcgt
    12781 ggatgcggat ccagcgcatt tccggcaact tgtctggaat ctcgttcgta atgccgttca
    12841 ggcaacctct ccgggcagca cagtcacgct tcgtctcgag acggtgggct ccaccgcttt
    12901 tctgctcgaa gtggaggatg cgggagcagg gattccggaa gagattcggg ctcacctttt
    12961 tgaagcgttc gtctccaccc gggtccatgg ttcgggaatt ggccttgcgg tcgtcaaacg
    13021 tatcaccgag gaacacggct ggacgataga ggccgtgggc aatcgacatg ggggagcgac
    13081 atttcgtcta tcgattcccc ggaatcagcc gtgatcttag cggtctgttg ttcttgctag
    13141 gacggtcccg cggcggcatg ctgggcgacg gagacgttca tcagcaaacc atcgagccca
    13201 ttgaatggaa ccgtgttgga attcgtcttt catggtgtat tttcagtcat cgaatcaggt
    13261 ttggcattct gattgcttgc gttctgactg aacccgctct tcgccacgca gtggaaagga
    13321 tatcgcagca atgaattcac ttcgaccctc tttctttggt gccctcgctc tgacttttgc
    13381 gctgtctttc ggtcagcttg cgcatgcggg acctgctcag gacttcgttc gtgccaagca
    13441 caccagtctg acgaccgagc ttggaaaagc tccttcgccc gcacgccaga aaaacgtcga
    13501 gcatctcatg gacggctttt ttgacttcga caagttaacc aaggatgccc ttgccaggca
    13561 ttacgacgat cttacggagt ctgagcacaa cgaactcaaa gcgctcgttc aggccctggt
    13621 tacgaagaac taccgaaaaa acattgaaaa aacgcttaat tacgaggtag cttttcttgg
    13681 cgacgaggcg atcgacggag gtaagggcca gaaggttcga acccgcgcgt cgaagcgaaa
    13741 ctcgggggat gccccggtcg aaataacgta ttatctccac aaaaaaggtg atgcgtggat
    13801 ggtgtacgat gtcgagacgg acgggtcctc gatgttacgt aactatcgga accagtttgg
    13861 gaaagtgatt cgccgggacg gatttggtgc cttgcttgcc aagcttcaag accgtctcga
    13921 caagacatga ttgtgtacgc cagggtgttg catattcgcc acacccgctt gtaacgtatc
    13981 cttcaaattg gtctccgttc gcaagaaccg atacgatcaa cgggcgcccg gagacatgag
    14041 caagcatccc gcgaaggtat cccgtaagag cgctcccagt tcggagccga aggttcttat
    14101 gcaactaagc gcctgtcggg gtgggcttgc cctcgagctg gccgagcctt ttaccgtggg
    14161 tccgttgacg gtaacgcgtc tggtgtcgtc cctgcagggc ctgtcatttc cgatagattt
    14221 gacaggggga gtagggcgtt ttcgtcatcg tcgcggcgtt ctcgatcatt tggttgtgga
    14281 aagccaattg gcgcaactag ccatggcgct acaaccgcgt gcgcgtgcgg ttttcgattc
    14341 tcccaaactt cggctttcgc tacttcccac ggccctgggg ctttcggtgg gtgtatgcga
    14401 tgaggacagg gccctcagtt tttcggcgca cttcacttcg gatgggccga cgcttcaatt
    14461 cgtggttagc gatgcgcgcg cggtggggtt tggatttcac agtcatgccc tcgcggtgcg
    14521 tttggcgcga gcgctcacgg gttgcacgca atcgcaggga agcctcatca gcttggagcg
    14581 tgggatcgcg cacctggtgc gagaggtatt gttggacgcc ggggctcgtc ttccatcgtg
    14641 tcaacagtta cagtacgcat gggacgagac gttggatgga attcggttga cggctagttc
    14701 agcggccccc gaggcttgcc cggcggcgga agtgctgcgt gcgatcgagt tggcgaccat
    14761 cgcgcggcag ggggatgagg cgcttgtagc gggaaatttt gacgatgcgc gagcggcgta
    14821 tgtgacggcg ttggaatccg cgccgcgtca tcccgatctt gcgcttcgga ttgccgagat
    14881 cgacttgttg gcgggatacg caccggaatc cgcgcttgcg tcgctggtgg agacgatgcc
    14941 ggcgcttgac ggtggactca ttggcgctga acttctcgcg gcgctcggcg atcgacaagc
    15001 atcggtcctc gccttgcgac gggcggcgga tcgtgaacct tttgcgccga tggcagctcg
    15061 aatgctcggt cgggcagcag ccatcaccga agagcgttcc gaccgcttat cgttgctaga
    15121 tgaggctctt gcgcgctgcg tcgggtgtga gtcgattcgt tggcagcgag cgatcacaag
    15181 gttggaagcc ggctgttttt cggacgcgat cgcggattta ggtcatctcg aagcagcagc
    15241 ccagggagcc gaaaaacgct accaggtttg tcttcgcgcc ggacaacacc ttcttcaggc
    15301 acggcttgcc ctggacgccc gcactttttt tgaaaaagcc ttgcgctatg cacctcgaag
    15361 tgcgcaagca agctcggggc tcgcacgcgc attttatgcc ctcggcgatg ggcgtcgcgc
    15421 ggtccgtttg ctcacccgag ccacagaact ttccggttcc tcctctccac ccccaacgac
    15481 tttgctggag ctagccacgg cgatcgtgga attcacgcgg gattatcctg cagcgattta
    15541 tcatgtgcgg tcgattccct tcgggttacc acaaacggtt gctgcacgtg ctctcgaagg
    15601 cagatggcgc aatcagctcg gtgatatcgt gggagcgagc catgcgtttt cacaagccag
    15661 agaagccgcg gcacagcttc cccgagacac gatcgcagag caatcccaat ggctcatcga
    15721 agccgcgcaa ttcgaattgg atatccgcaa cgatcctcgc gtagccaaac gccacgtaga
    15781 gcttgcgctt caggccgatc cgaacaatcc acacgttcag caggtgtttc gtcgagcggt
    15841 tcgggaggaa caacaagccg tcgcgacacg gatggatccg gtggcttccc ctgagagcca
    15901 cgacggtgcc gccgcttctg attcgcctct cgtcattgcg gacgaaccca taacgaagtc
    15961 cgcggtgtcg agtaccaact tattcgaccc ggcatcgact tccggagaga tatatgacga
    16021 agggccactc gatgcgaacg aagaggccca gatcgaagtg ttggaagacc gggtactcgc
    16081 gaatcccaat gaccaagaaa ccataagaca attgagcgaa ttgctcgatc gaggaaagca
    16141 atacccaagg ctgctggccc tggtttcggc gcgattggac gagacttctg aaccatctta
    16201 tcgggccgaa ttgatggcaa tgcggataca cgcgttacag cagctcatgg ctgcctgtcg
    16261 cgcccagggc aacgaagggg aagcggagtt atatgaagcg gctttggccg acgctactga
    16321 gtcgagttcg gacgattccc tggaaggata gcgggaagac ctccgatggc acggaaagcc
    16381 cgatgaatgg ctcgggtgat ggggcccact tttcccgttc caatcgttcg atcatcgatt
    16441 cgcgtaaccg gcaacacctc tcgggtcgtc gacgacacga atacttcatc tgcatcgagg
    16501 agttccgttt gggtcaagga cgtttcgcgc agctcaagtc cctcgtgcgc tgcggctttc
    16561 aaaagatacg atcgagtgat tcctgataga attccttcgt ttacgggtgg cgtcctaatc
    16621 acgccggagc gaacgatgaa gacgttggaa gaagctcctt cgagcactct acctctgcca
    16681 tcgacgatga tggcttcttt cgctcccacg gcgtgcgcct gtcgtaatgc cagcaagctc
    16741 accagatagt tggcaacttt ggcacctgcg gcggacgtat tatctgtcgt tcgctggctt
    16801 tgaaagagag cggcggcgat accatctcgg tattgatcct ccgagggaac gagcaaaggc
    16861 accacgatca tcacgcgcgt cggttggcgt gcccccgtgg gatccagtcc ataaggtcca
    16921 attcctctgg tgatgtggac acggatcaac agattgggaa gctccagcaa cgaggccgca
    16981 tgcaagattt cttgtgagag tcggtctttg gacacgggga tatcgatgaa aacctgctcg
    17041 gcggatcgga acagacgatc gatatgttcg cgaagcgcaa atatttttcc atcgtaggtt
    17101 ctcatcacct cgtagatcga atcgccgtac agaaagcctc gatcgaatac ggagatccgt
    17161 gcttgatctt gggcgacgac ctgaccgtcg atcataaccg cgaagatttc tgattctgtc
    17221 atgggtttgc gtgaaagtgg gagagggtgc agggcgcgct cttcgtagcc gaccagctta
    17281 gcaggaggtc atgggcgcgt tgcgtggtgt gatcgatggc gcatgccaga tcccgttcat
    17341 tgattgcgct tggatttgtg atgggtttgc caagcacgat gaccatgcga ctgaacggtc
    17401 tcggaatgat gaaacgatcc caggaagcga ggcagaagcc atgggagcat gcggcggcgt
    17461 agggaacgac ccaagcgtgt gcgcgatagg cggcggcaag ggcaccaggg cgtgcgactt
    17521 gtcgcggtcc acgagggcca tcgaccgcca atgcagcatc ccatccttct tgcactcgtt
    17581 ggacaatcgc ttgaagccct tgccttcctc ctttcgaact cgagccccgc atcgaaaaca
    17641 ccccaaacca acgcatggcc caggtcaaac gctcaccatc tggggaatga ctgaccaggg
    17701 caatggttcg gcggcaccta cgatgggcaa ggagaaggag caattgcccg tgccaaaggg
    17761 cgagaacacg tggctggctg gaatcgttga ggagaggatg ctgaaagacg cgaaggcgaa
    17821 ccgtgagcag ccaagctcgc gcgaccaaac ccaatgcgag gccgacgagc atcgagagca
    17881 tgcgcttatg ccttgagctt ctcgtagagt tcttttgctt tttcgaaaga agcgtcgttt
    17941 tcgagggctc gctcgagcat ctggatggct ctttgcgtct ccccttgtcg gtggctgatt
    18001 tcaccgaggt agtagaacac ctcagctttg gtgatcggcg ccttatcatc caaacgttgg
    18061 agtagtagag cccggaaagt tttctgcgct cgatcgagat cgcccaagtc gagagccagg
    18121 cgtcccagat cgacgagaat ggcgaggtta cccggtgtga tgcggaacgc ctgatcgagt
    18181 tcggcgaggc cttgttcctt gttgccttcg gaaacgtggg ccttggccaa acggtgatga
    18241 atggccgcca attctttgac tcgttttccg ccgtaagact ccacgatttt ttcgagcgcg
    18301 gagatggcat ccttgccacg tccggaggcg ctataggcgt cgcacagcga aagcagcaac
    18361 tccctatcgt cgggggccag ctcgctagcc tggtcgagga gtttggcagc cgtggcggca
    18421 tcgttacgtt tggtcatgtg aatatcggca gcccgctgaa gcaacttgac cttgtcggca
    18481 ggatctgacg cgttttccgc atcttgcgcg agcatggcgg caacttccgc ccaagcgccc
    18541 gtccgctcgt acagctttct caacatctcg cgcacttccg cggacgccgg attggcagcc
    18601 aagcctcgtt cgagcacgcg gcgcgctccg ctttcgtctt ccatcagatc gaagattccg
    18661 gcaagacgaa gagccaaggt gaccgcctca tcgccttcga gcaaagcaag cagccgttcg
    18721 agtgtttcga ccgctttctg tttgtcaccc gtggcttcgt acagacggcc cagcgcctcg
    18781 agggcttgtt tatgcgatgg gtccctttcg agtacggccc cgaaggtgtc gattgctttc
    18841 tgtttatctc caagtcgtgt ttcgtagacc tcacccaacc ggaccgtgaa ggtaagttcc
    18901 gcttctgcct gtccattttc ttttgcgagt ttgatttgcg tgtcgagcaa gtccgcgagg
    18961 tcccttattc tttccgtgcg ttcatagagt tgtgaaagaa ccacgacggc atcggtctgt
    19021 ccgggttcct catccaacac gctgagcagc acgaagacgg cctcatcgac ttggtcaaaa
    19081 cgagatacga gcaaatccac caattccaag cgaagtttcg tgcgttccga tgcatcggaa
    19141 gccacatcga tgagacggcc cagcaattcg gacaagtcct tgaaacgctt ttggtccgca
    19201 tagagttttc taagagccga agcagctttc gaatccatgg ggtcggcttc gaacacttct
    19261 tcatagattt tcaacgcgcg ttccggctcc tggagctggc tgctcaacac ttccgcggct
    19321 tgatggcgaa gctgcaacga atccggacca tgggccgtca gctcggcacg cttgagcaga
    19381 agctccacca cttccgcaaa atcctctttc ttttcgcgaa gttgagttag ctcctcgtag
    19441 ggccataggt aaccatcatc gagttcgatg agttgtcgaa gcacttgttc ggtaagttca
    19501 tcatccgcca acacggcgtg agcgagcgtt ttggcctcac ggaacaactc acgacgtttt
    19561 tccgtatcga ggtcaaggat ggcgcgacgg cgcaaggtag cgatgagatc tcgttcgcga
    19621 cctggttccc gctgaagttc ttcaatggct tgaagcaact cgaggttgtc agggtcgaca
    19681 tggagcgcct gctgcatggc ggactccgcg gattttccgt ccgccaggcg tattcggtac
    19741 cagtcagcga ggcgcacgta cagatctcgc agggtcatgg gatccaaacc gtcggccttg
    19801 gaaagggccc cgtggaaagc tttgcaagct tctgtccact gctggttggc gccagcgagt
    19861 tgctcgagtt ttgcaaggac gtcggaatcg cagacgtcat cttcaagggc ggcttcgatc
    19921 acgcgttgag cgcgcttcgg atcttcggct tgctcttcca gaacgcccgc gagatcaaga
    19981 cgtaagcgca gtctttcgga ggggtcttcc gcgaacgaaa cacgtttttc gtacagcgat
    20041 gccaggcgcg agaagtcgcc tcttgcgcga tacacgcctt cgagagcagc cgcagtgtcc
    20101 tcgaacaagt tcttgtcctc ggtaagtttc tctaacgtat cgcaagcttt ttcgtgagta
    20161 ggaacatggt ccaaggcctg gcggaggttc tccaacgccg tcattttgtc gtcgaacgac
    20221 tcgagctgaa gcacggcgat gcggtgatat agatcggctt gtccttccgg cgtcgtttcc
    20281 acatggactc gtcggtcgag aatttcgttc aactctcggg tgttgcccaa cttggagtgc
    20341 aatcgatcca gcgaagctag caattcaggt tcatcaccag cttgatcgag ggcacgagac
    20401 agagcctcga cggcacgttt atcgttttcg agtttttgct cacagattcg gccaaggcgc
    20461 ttccacaaat cggtggcgag agcggcgtcg aaagtcttgc cggcacgttc ttccaacacg
    20521 tcggcgtatt gctggaagcc gttgctggcg gcttcggcca ttcgttcgat atcgtcgtgc
    20581 aggccggtat cctcgggggt ttccgccagg gcacgcagca atgcctcgag ggcgcgctct
    20641 gtgttgtgaa gcgagtcatg caagacggtg gcaatcgacc gcaaggtcaa cgctcgttcg
    20701 gcaacatcat gttgtgcgag caagcgcatt tccatgacag taaccaacat atcgaattgg
    20761 ctggtattgc gcaggaccgg ctccaagata tccgccgcgt cgaggcgcag atcttcgaat
    20821 ccttctccaa tttcgcgtac ggcttcgatc gtgtctcggt cctctggcgc ttcttcgagc
    20881 accattcgaa acgcttcgag cgcttcggtt ggctcggcca tctcggaggc aagtagtttt
    20941 ccgatttccc gtcgaagcga ggctcgctcc tttttatctt ccgcgatggt ggcttcttgc
    21001 cgtaaggttt caagcagttc gggccacatt ttttctacac ggaacagcgt ttcgagtttc
    21061 gacagcacgt ttttgtccgc gccccgcact tccaacgcct gtcgcaacac gtcgatagcc
    21121 gcctggttat cctcgaggtc tttctgatac cggttcgccg ctttgaggag catgtcgtat
    21181 ttgagatctt cctcggcctc accttccgca agttcgacgc gccgctgata gagttcgacg
    21241 agtttatctc ccgcattgcg cgcctctaga agatcgatca acgcatcgat ggtcgatgca
    21301 cttgcgggtt ccaattcgag agcgtgctcg aacgcttcga tggcgccgtg cgcatcttcg
    21361 agcatgtctc gtttgcattc tccgactctt ctccatgcca aagcgcgatc ttcatcgctt
    21421 ccgatcagct cggctttttt catgagtacg gagacaagga cttgccaatc cgcgagcagc
    21481 atcgatagca cgtccatttt ttcgagcggc tcggcatccg tttcgtccaa ttggtgcaat
    21541 cggctgtagg catcgagggc acgacgaggg tcatctcgtt tttcatcgtg gatggtggca
    21601 agagccgaaa gcaggtcacg cttgccgctg tgttccgcag cttcaatacc ttcttcgtat
    21661 gcctcggcaa gctcatccca cgcgccaagc gtccctgcca gccgttccaa ctcctgccga
    21721 atgtccatgt cttcggcatc gatttcgaag gcggcgcgga acgcatcgaa ggcacgttga
    21781 ttgtcttttc ctctcaactc ccacaaacgg gcagtttcac ggagaatagc aacgcgttcc
    21841 agctcgtccg tggacaggtc gaagcgctgt gcattgaggt agatttgctt gcgccaatca
    21901 tcggcgccct cgtaaagcgg cagcaagata tccacgacgc gcgctttttg atcttctctc
    21961 gccagtagcg cctcgagttc ggcaatggcc aggttattcg agggaacctg ccttacgatc
    22021 tcctcgagct gatcgaccgc cgaatcggcg tcgttcattt catcacgata caggcgcgcc
    22081 agagcgagac gatactcaca agcccgatcc acatccgaat cgttttctgc ccttcgggca
    22141 tacagctcgg cgagatcggc gaagcgttcc cgttgtcggt acagctcggt aagagcgtcg
    22201 agggaaacac tatcgttttc atcgacatcg agagcggcgc agtaggtttc aatggccttg
    22261 tcgggctctt ggagggccgt acgttcgagt tcggcgatcg tgtgaagtat ccggatgcgg
    22321 tcttccggtt cgaatcgatg atccagggct ccttgataaa gctccacccg gtctttggct
    22381 cgctgggcat cgatgagcaa cttgtcgatg gcatcgaaca agacagtgga atcgggttcg
    22441 aaagccagtg cgcggcgata atgcagcagc gatttttccg tgtctccgag agaggcaaag
    22501 agttcgcccg tgcgacgcgc caggcttgcg gtggcttcat catccgacac cacgtgttcg
    22561 agttccgcgg cgaaaatctc ggccaagcgc cgctcggcac ccgccacgcg ggcttggctt
    22621 tcgagctgct tccacgtatc acggtccgta atatcttcat gaagcagctt tgccgtcgtc
    22681 tccaaagccg ccgagaagtc ctcttcctgc tcttcttgca tgacagccaa acgacggagc
    22741 aattcccttc gtttatccgt atcgggacag gcatccaatc gcgcggcgat ggttcgtttt
    22801 acctttttcc aatcggccct gccgaggtag aacggttcga gcatttccgc ggcacgagcg
    22861 cgattttccg ggtcttgagc actgtccagc agtcgctcga gttccgcaat cgcagcctcg
    22921 ttttggctgt cctccgcaag cgcttccccc aactcatcga aagcacgcat cgtatctccg
    22981 agctttcctt ccgcgacctg ggcgatcttg acgcgaagct cggccttgcc ctccgtgctt
    23041 tgctcgagtt gccgttcgta cagggagatg agatcggacg ggcgctgtgc ctgcgaatat
    23101 agcgtttcga gcgcctccac ggctgccgga tcgaggtcga tgtccaaaac ggtttcgaaa
    23161 acagcaatgg cgccttccga gtcttcgagc acgtcgctac acagcttcgc ttggcggaac
    23221 aacaactgtt ttcgttcctc atcgttctcg gccacgtcca cgcggcgacg gatgacgtcg
    23281 agcaatgcgg tagcatcacc gatttcctcg tacaacgatt cgagggcaac caaagccctg
    23341 cggtcgtcac cacgaatatc caacgctcgg atatagtgtt cgcgcgcgag ttcgcggtcc
    23401 gcgagttccg tgcgcgccag ttcagcgatt cgtaacgtga catccaattg tacatcgccg
    23461 tcgagaatgt ccgtcacgac ttctcgaagg agagtgacaa ggtcggagaa gcgacgtgtc
    23521 gtcgtagcca atcgttcgac gatttcgagc cagattgtga tttcttcgct tcccgccgct
    23581 tctttggtac cccgcacggc gaatccgaag gcgcgggagg catcgtcgag cggcccctcc
    23641 gcaacacgaa ccgcctgttg aagcagcgaa agacgctcgt cgggattcaa agtggtgtcg
    23701 gcttcaatct caacgacacg cagcagtttt tcattgtcac catcggcttc gtacaacggg
    23761 tgcaatacct gagccgcctc tcgcttcgct ccttcttccg tcagcaagcg ttccagagcc
    23821 tcccgactcg gtttatgact cggatccacc gtcaatgctt gccggaaagc atccagggca
    23881 ccgtcgaggt cttgcaaatg ttcgcggcgc acatcgccca acctcaccaa taaggcgagc
    23941 cgatcggctg gctcatcggc caggttcaag tgcgtgtcga tacaatcgga caagtccgtc
    24001 caccgctctg ctttttcata aagtgtttcg agcgcttcca gggtcgaggg gtcgggaccg
    24061 taatcttcga cgatggcgcg ccacgcaagc gtggcttcgg atacatcctg gagctgattg
    24121 gttagggttt cggcgatacg aaccatcaag cgacgacgtt catcggcgtc ttcggcgccg
    24181 tcatgacgcg ttcgcaacac gctcaccaag tcccgccatg cttggacttt ctcgaacaac
    24241 cgttcgagcg ccacaagggc ggccggatcg acggggtcat cctcgagtct cgaccgccag
    24301 gcatcgattg ccccaggcca atcctccaag atgttttcgc agatctcgcc cagtcggctg
    24361 tagatttcgg ctcgacgctg ggcatcatct tccagtttta cctcggtgcg aagcacttcg
    24421 accaaggcaa cgtggttttc cagacccgca taaatttcct ccagcgcccg tccggcaggc
    24481 agcacgaggt tcgggtcagc ggggtcaatc gccaacacac gacgatacac ggcttcggcc
    24541 cgttcgacat cggagaggtg atctcggtaa atggaagcga catccatgag gatggcgcct
    24601 tgaacatcgg gggtaaggga aagcacggcc gcttgtgtca gcaccttggc tgccgtctcg
    24661 tgctctccaa ttctcctacc gatgtcgatg agtcgggacc gtgcgtggcc atcaatcgga
    24721 tcgagtggca caagcttggt caaggcttca aaagcacctt gatcatcggc aagcctttca
    24781 tcacgcaagg cggccatgcg ccggagcaat tctcttcgct ccgattcatc ctcggtcact
    24841 tcgaggcgaa tatcgagaac acgaaccaga tccctgattt cgtcgcgatt ttcgtaaacc
    24901 tcttccaaca ctcgcgcggc acgagcgcgc aacgccggca ccgtaagcaa ccgttccgcc
    24961 aagtcgcgcg cttcctgctc gctcgggcga agatgcaaga cgtcttccac gtggggcatg
    25021 gcgttgacag gattgccaag gttatcgagg tggagctttc caaggcgcaa cttgatggcg
    25081 ataaggtcgg attcccctgc ccgattcagc cgaccttcca acaaggagac cagtttgtca
    25141 taccgttttt ccgaagcgta tagcttctcg agagccatcg tcgccgtacc atgcagcgga
    25201 tcgatgtcca aaatccgttc gtaatagtcg atcgacttgc tcggatcctc gatgatgtcc
    25261 tcgcaaatca tggcaacttc agcaagaaac tcgatgcgac gcgcgccgtc ggtggtaccg
    25321 gtgatggcgc gagcgtataa ggcttccagg tcttgccaac gttcacgcga catgaggatc
    25381 tccttgagat ccttgaaggc ttcctcgttg gccggctgac gagccaatac gcgctcgagg
    25441 tacggaatgg cggcatcttg atcgccaata tgctcacgac acagcttcgc cgcgcgctga
    25501 cacaattcca cctcgatatc atcgggcaac gacaattgcg tatcgagaac cgatcgatac
    25561 acctgggcca gttccccagg cctttcgacc acgatcgaca aatctcgagc acgatcccaa
    25621 agtgaaacct gctcagggaa ctccgtcacc gctttgagta cgacatcgaa agccgtactc
    25681 gcatcgcgca ttttcaattc atacacgtcg atcagccgaa ggagcagttg caaacgctct
    25741 tgggaatcgg tcgtggcatc gacgagcagc gcaagcgcat ctgcctcgcg cgtgggatct
    25801 cccaaatccg catgcacacc caccaacagc gacgcagcac gcgcgcgtgt ttcctcggtt
    25861 cctacaagcg catcgaggac agcgatcgct cgagccgagt ctgcatcatg ctccagcgcc
    25921 cgttgtaccg catcgagggc atccaagggt cgttgcaaac gctgcacata caattccgca
    25981 agcaccaatt ccaattcccc ccgctccgct ccctgctttt gatcccgatg ggtttccacg
    26041 atctgcgccg ctgccgcagc atttccttcc gccaaaagca gccgtggcaa cgatgtgagc
    26101 gcttgcgtat tggaagggtc caactgcaac gttcgacgat acaaggcgat ggcaccgtcg
    26161 ggcgacgaga acgcatcttc ttcgagagtt gcccattccg taagcagagc gattttggaa
    26221 gagtcgtcgg ctcgttcgat ccggagttcg aacagccagc gcaagtcatc ctgccgtgcg
    26281 gcagcgcgca agattcggtc cagggttttc aggatatcga tgtcggaagg gtcttctgcc
    26341 acgatctgac gataggtctt gatggcatct tccattcgac cgaggtcgga ggcgtacaca
    26401 ttggcaagcc gaagctgaag cgcgcggatt tcgtccgctg gcatatctgg gtcgttggca
    26461 atccgttttt ccagcgcttc tacgaaaggc tcccacaaag ccgcagccct cgcataccct
    26521 tcgagcagtt cgagagcgcc ttcagcatcc ggtgccaagt cgaaagcgcg gcgggcatac
    26581 gtcaaggcgg cttccttgtc ggacagacgg tttcccgtca cgtccgcgag tttgcgaagc
    26641 aactcgagtt gtcgctcggg gtcttcggaa cgcgcgagca ggatttcata caatgcaggc
    26701 agacgactcc aacgctcgtc tttttcatag atgggcacca aggcattggc cgcgcgcaag
    26761 tcatcgggcc gtacgctcaa cacgcgctca tacgcgcgaa aagctcgttc gggggcctga
    26821 agcttcgact caaaaacctc ggcaactcgg aaggatagat cgactttcga ttcggtcacg
    26881 gtcgttcgat ccgcggccga tgacaacacc tccgcaagcc cctcccaatc gtcattttgt
    26941 ccgtataact cagtgatcgc gtcaaaatcc tccgcggcca agtaggcgtc gcgaagcaca
    27001 cgcatggcgc gagaatggtc cggtcggatt tcgagcacac gacgccaggc cgaagcagcc
    27061 cggcgagggt cgttcagcct ttccgcgtat acgccaccaa gcttctgcag cacggcaatt
    27121 cgggcgtttt cgtcttcggc tgtctgcacc cgtcgctcca gcacttgcgc taccgtttcg
    27181 taatccttgt cacgttcacc ctgtttttcg agggcatcca tgactcccgg cgcgtgcgga
    27241 tcgatctcca aaatctgttt gtaaagggcg atggcatccg cggcacggtc aagtctctcc
    27301 gctgccaatt tggccatctc cgtgagcagc cgaaccttcg tctcttcatc ggcatactcg
    27361 acttctttgc cgagaagacc gaagagctgc ggccaagcgc ggcgacgttg gtataggtca
    27421 cgcaaccgct gcctggcttc ttcatctgtt ggatccaagg cgaaaacgcg ctcgaacaca
    27481 tccgtagcgt tttgcacgtt ctggaattgc tccatccacc gctttcccgc cgatcgaagt
    27541 aacgaaagtt tctcttgcgg atcgtcggaa agctccgcga gtttctgctg atgaaccaac
    27601 aggtcccgcc atcgctgcaa ggactcgtac acacgacaaa gctctcgcac ggccgccaca
    27661 tcgctgtcgt cgagggccaa gatttggccg agtacggtca ccaaagcgct gtcgctcttg
    27721 atgtggtcac gatataccgc cgcaatctca cgaagaactt cgagacgccc ctgcgcatcg
    27781 tccgcggcaa tggcatcgag gtcctgccga agaacgtcga caagggaagt ccaggcactc
    27841 gtttgacggt acagacgctt gagcgcacca cgggcttcgc ggtttccggg gtcctgacgc
    27901 aacagagcct tgtattgttc gatggctttg tggacgtctt cctggttctc ggcgagttga
    27961 gcgatctccg cggcaagctg tgctttctga tcgccatcgg gcaaagagcg ctgagcatcg
    28021 gtcaaaatgg tgatgagtcg ggaggtatcc ttgcgctgct cacaccacgt tcgatagaaa
    28081 ctgagcatgc caaggtgagc gggttcggtg cgtcgcacct tgtcgaagta cggttctgca
    28141 aggtcgggtt ctccccgcat gcgccaatgg accatggcga tttgaagcag gatcccgagg
    28201 tcctgcccgg acttgaaagc accgcttcga agatgatctt cgtacaaggc aacgagatga
    28261 tcccattgct cttcccgact gaagtagtca gacaggaagt tcatggcctc cggataccca
    28321 gggacgaggt cgagcgctct ttcgtaggca gcaacaccac gcgcttcggc cccgagcttt
    28381 cgtacgacga ctctggccaa gcgcaaccac gcggcgaacc tttcctctcg gaccggtgac
    28441 tccgttgcca gaatttcgag gattcgacac gcgtcgttcc atcgctccac ctgtcggtag
    28501 acacgctcca acaacatgca ggcacgacga ttgcggggat cgagttcgat ggcctgttcg
    28561 agcaaagcca tgagttcatc aaggtcgagt tgctcccgcc cgaaccggaa cttggcttcg
    28621 gccacccaag acagcatcga gcttcgaatg gacgaatcct cgactccttt ggcctcttcg
    28681 agctttcgtt cggccaattc ctcccaaacg gcgcgctggt cctcgacacg cgccatcgct
    28741 tccgtggccg tcggatcgtc cgggcgaagg tccagccacc gcttataaac atcgaccgct
    28801 cgcttgtcgt cgagtaactc gtcctccaac acacgagcga gttccgccaa ccgtgcgact
    28861 tcttttgagg aaccttgaca ttgagcgatc tcatactcga gcagattggc gacagcggac
    28921 cactctcgcc gggtttcgtg ttcccttcgg gccgctccga gcaggtcgat cagttcctca
    28981 tcggataatc ccacatcagg cgaggtgagg gcatcctgca attcgagcca ggcagcctcg
    29041 ttgtcttcgt cgtcttgcaa caacccaaga gcatttcgaa tcttctgcga gctcatcgtc
    29101 actccatcca ggaggtcgtc gttcaacagg aacgagattc ggttcgaaag cgggtaccca
    29161 aatggcacgc caacggctcg tgcccttttc cgcgcttcac cgagtcttcc ggaaaccaat
    29221 ggtcccagga aagggcaccc gctgagggtc aggatgctac gccgcagggg tcgtggatac
    29281 aagacacgcc agtcgttaag gcggatagag aaggaaatcg tcaaccggag cttgactttg
    29341 ggaacgggat cggctttcgg gcgagggctg acgcgctatt attcatattt ttctaagtag
    29401 ttatacaata tatatattcc ttatttatga tttcgaattg gaagcgtcat ttcgctgatg
    29461 acagggaaat aagtttcacg gtttcccctc catcgcaagc ttgcgtttgc ccgcgctcga
    29521 tgtgctatga tgcggcaagc tattcgtagc gttgccgatg ccgatggcgc gttacgctca
    29581 tcacgcgaga cttccgatgc acagcctcca gaaggtcgca attattcttt gcgctcttat
    29641 tgctacactc gttctcattt tcaccattgg aacgactgcc agagccgccg agccaccgac
    29701 agcggatcaa gtcgcttccc gtgttcaggc attttacaat cagacccgaa cctttcaggc
    29761 tcggttccag caagactata ccgtcaaggc gttcaacaag cgcaagcgtt ccaccggaca
    29821 agtgtgtttc gaacgtccgg gcaaaatgag ttggaaatat gacaatccca acggaaaccg
    29881 ggtggtatcc aacggcgcga cgttgaaagt ctatgaaaaa gacaacaaac aaatgttcga
    29941 gcagcccatc aacgattccc agtatccagc cgcgctcgcg tttttgatgg gcaaggggga
    30001 tttgaagaaa accttcacct tacggcttct cgatgcaaaa cggatgaact tcgaaggggg
    30061 gtatgtgttg gaaggcaccc ccaaatctcc cacccctgcc taccaaaaag tgttgatgta
    30121 cgtcgacgcc tccacagcgc aagtccgtcg catgctcatt ctcgacgccc aggggaaccg
    30181 gaatatgttc accttcgaca accccgtggt caataccagc attccatcgg gggaattcaa
    30241 cttcgatccc cccaagggaa ccacgatcgt tcatccgtga tggcattggg gcgcggtcaa
    30301 cgccctcccc ggtgaaccgc acgttcgaaa aagacttgtt gcgcatgggt gatcgccacc
    30361 gccaaagcat ccgtggcgtc aacccctggc aattctcgaa gacccaacac ggccttgacc
    30421 atcaacgcaa cctgcctttt atccgccctg ccacgacccg ttaccgctcg cttgacgcgt
    30481 gcgggctcga cctcgtggat gggaatcgcg gctttggcaa gcagaagcaa gatcaccccc
    30541 cgggcatgcc cgagtttgct ggccgcttga gcatctttcg caaaaaatag cgattccacc
    30601 gccgccactt ccggctcatg gacccgcaaa atctccccaa gcgcccggtc gatctgcagc
    30661 aaacgttcgg cgatcgacaa tcgcccgtcc aggtccacga taccgtaatc ttccgcgctg
    30721 agccgtgtcc cctgcactcg cacgatcccc catcccaaat gtctcgttcc agggtcaatt
    30781 cctatgacgc gcatctacac tctcagcgga aatatcttac cagggttgag caaccctttc
    30841 ggatcaaata cttttttcac acgttcctgc aacgaaacca gctcatggct ctgctccaat
    30901 cccaaatacg gggccttaag aacgccgatt ccgtgctcac cggaaatggt gccccgcaaa
    30961 gcgatcacat ctcgaaacaa gttttcaatc gctccaagca cgcgaggtag gtccgccggg
    31021 tcatcccaaa gaaagttaac gtgcatattc ccatcgccag cgtgaccata ggtcaagatc
    31081 ctcaccttat accgttctgc aagagcctcg gttttttcta gtaaagctgg aatctgagat
    31141 acgggaacca ccacatcttc ggcgagtttg tacttcgcca gctttctcac cgcgggcgat
    31201 agctcccgcc gcgctgccca aagccgggcc gattgcgtcc ctgcttgcgc caccaacacg
    31261 tcgagcgccc cgacggctac gcaggcttcc cccgttcttt ccatatctcg ttcacaagcg
    31321 ggcccctcgc catccacctc gatcaaa
//
LOCUS       DAOSOR010000005        27087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_1779_length_27087_cov_4.758175, whole genome shotgun sequence.
ACCESSION   DAOSOR010000005 DAOSOR010000000
VERSION     DAOSOR010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 27087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27087
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1779_length_27087_cov_4.758175"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1450)
                     /locus_tag="PK710_00595"
     CDS             complement(113..1450)
                     /locus_tag="PK710_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08229.1"
                     /translation="MMTDPGRWEERIEQAFTEYRLGLVSRRLGGWRSGDIGPLVAYRS
                     LTTRERFEQMAGYPKEVPNADSMLRWQAYLTIERVTWEERAAVEVLRRSFDHRVDGLG
                     EVAWSLRSLVLEMLASRDLGRRANVARGLEQASAEASESALKWAIRRHGAAYHLGTEG
                     LEWLEVPLRDMGVRQVAERVMAGTDDAMAAGIKAESWQEVVRVAAAMDARSGWPAALT
                     PRWFRWMFGGWKSLQGIHLELGPLCDPICGASFARALARFGGAIHRGWAMRSQGPFSM
                     VHRPFDAAAASYGALLASLLTSVPFLKRRMGLGRDAARIQARSLGLSLLISVRMNAAQ
                     AIVATMTSPQAMVEAHVTEVSKAMHASIPETLAGVVPRYEPRAASRLCGALHAMTRLR
                     ELVERFDDDWFDNPQAHEFLSEIDISERLVLDEHDVRQGVENMSGWLSEWLLG"
     gene            1554..2075
                     /locus_tag="PK710_00600"
     CDS             1554..2075
                     /locus_tag="PK710_00600"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HOT08230.1"
                     /translation="MNIDDELEKAIQQKEQGSARRPRRDAPTPKRNLGLLIGLIVIAV
                     VVLALLPFTFKGSAVWATTVDQLVSEPDLKGRRVRVEGILVKGSLMKRDSPCEYRFQL
                     RTNEATVQVRYPQCVIPDSLQDRPEADITVTAEGTLGQDNVFVASQVLAKCPSKYEQK
                     DGKTISSHQSSSR"
     gene            complement(2256..3080)
                     /locus_tag="PK710_00605"
     CDS             complement(2256..3080)
                     /locus_tag="PK710_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOT08231.1"
                     /translation="MNTADIWNFLVTQGGDFGLKLLTAIIAWVVGRWLITFSVTLLGK
                     LLRRGGKIDETLANYLKSITIVLLNIVLILAILDIFGVKTTSFAALLAGAGLAIGAAW
                     SGMLGNFAAGVFMQVLRPYKVGDFVSAGGVTGTVTELGLFATTLVTPDNVFTVVGNGK
                     IFGDNIHNYSTLPFRRVDCVAKVANSVNPLDAIERLKPVIAAIPNVSKDPAPDIEILE
                     FTPEGPKLCVRPYTHTDHYWQVYFDTHKAIVETFGAAGYPVPETPLAYRNSPVQAS"
     gene            3311..4921
                     /locus_tag="PK710_00610"
     CDS             3311..4921
                     /locus_tag="PK710_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08232.1"
                     /translation="MRFSGWKPGFRRKTTNALLLGGAMGVAIGMVASEARAKRYTKPA
                     PPMSSYIQNYGFRSPSNPSANLNAHNPQAHSQLEAYLLEGAWAAELTGDPVMVAWFAA
                     ADAYLAPWRMSNCGDYSWDIDFDCPVLPWGSNIWQVGLAVQVSDSYSRGQKAFSATHP
                     NETEAQVGNEVLKIAAQEKTFPTGLSIATMVSSPGGITAGMHNSYWFSVLLRDMAMSA
                     YLESPAATAWECYKSNSYPGWCEYYRKSANWQHYSDVLSMVIVSWNQIQEDWKKALVD
                     DVIIDDLDPGFKAPPGAKFLGEKGWRGHFTWREASSSSPVSASWTANLPVAGTWTVSA
                     FIPYSDKATVTKGKVTVEAQDGSHEATLDESQIGGRFVELGTWPFESKAVVRMTNQGT
                     STECVGWDAVRFHLVSKASPDAGTPDGSAGSGGSGGSSGEDAHADVSGDETTEASADS
                     SHGETGIVSDANADHSDTDARDSGMSDAHPEAGNHPASWPSDQEADGCACLVARNQSR
                     RYAHLAVLALAAIGFGLRRHRRGHSLRS"
     gene            complement(4931..6883)
                     /locus_tag="PK710_00615"
     CDS             complement(4931..6883)
                     /locus_tag="PK710_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08233.1"
                     /translation="MDKKQQKMIVVGAAGGVALLSLVITWRVSASRAEQRAGELLSAA
                     ADMARCMAGDDVELQRTQVHQGFERRMVLDLPDINTAKPCLLALDVVKEKWDAYESTW
                     FNASGAKGQDGVAFKQRFDKAYEAFRALPLDKSSDQVFLRKEKYGSVLQAGELSFEML
                     DAARGMYKSEGASDAEIQKAGETRLRKAPNVPDRVAKGRAIATISGKVNPAHWRLIPS
                     GKGLMLHAVNEQGEMAVAWTDDGTTWKSARGPVGFAGKKDMEFRAFDAPGGERWFSIA
                     SGQKEQAQVQVGKIVEGQLPAPTSFPPPPEDWKRAPGGEREAVVLSQGTVAFPVWRIV
                     EKTNEEKKAEKKEREEWEKNFADKDVQALITLAEQRRVARAALGIDDDHKREDGIAYA
                     VANQPVTVLSLSGYGLAGLVPGAEPMALVGEGSLPAQKMASFSIPPPGEPIGMMASSV
                     VVKPVEPALRGSPWFRCVGSDGVYWGTTTTGSFLIGMQPNFLQLVQMTALADEGSHIG
                     CGPGVATVALPFLKDRIFANLLTIRGGELEGGKIHTTAGTDIALYNQTAATSAATGAV
                     VLGWVARGYALYTINNKTDNEFVAPRFLAEVGTDGSTISGLAFVGVGKRMVGMVARES
                     CPDASHCSTSFEILVSDDDARTWNPG"
     gene            7328..7921
                     /locus_tag="PK710_00620"
     CDS             7328..7921
                     /locus_tag="PK710_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08234.1"
                     /translation="MMRERKKKSWREIDQMRDGSAHRQRERDQERESIARANAATKQY
                     RNALEALFSPKAAEENQPVLPKTAARIVLPPVPEADPNHAERRRLLGKFLAATGPASI
                     SKAADMFFAAGFSLPEDQETHVQMLEHLDEKRVRDSIAKLVTIFAGEVPRRRPIVEQR
                     LRRIEEQADEHDTRVAAASLRRMLHGRGTGSPSSTSI"
     gene            7921..8343
                     /locus_tag="PK710_00625"
     CDS             7921..8343
                     /locus_tag="PK710_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08235.1"
                     /translation="MNKSEFESRFRDLVRTYASLRDNQGCIDCIGCERCSSCTFCRNS
                     KGLVRCHFCVACEDCSDCSHGRQCVGCLACHHCVGCERCVQSAYLIRCVGLTSCNYCF
                     GCVGLSHKDYHILNEPYDRKTYYEITQRISRELALTKA"
     gene            8348..8797
                     /gene="dtd"
                     /locus_tag="PK710_00630"
     CDS             8348..8797
                     /gene="dtd"
                     /locus_tag="PK710_00630"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOT08236.1"
                     /translation="MRAVVQRVASARVEVDGCVVGQIGAGLVAFVGAGRDDTDNDADL
                     LASKISGLRVFEDDLGKMSLGVGQVNGAILAISQFTVFGDVRRGRRPSFDDAMEPVQA
                     QRLFDRFVTRVRDTGIAVETGRFRAMMRVLVDNDGPVTILIDSRKVF"
     gene            8799..9131
                     /locus_tag="PK710_00635"
     CDS             8799..9131
                     /locus_tag="PK710_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08237.1"
                     /translation="MNDENNDPLDVLWNHVLTQWDNPKAHESLMQLGWQREQLGQVAA
                     WYRQQLDNPERQPTAQAMLQSLTVLATQQLENCRSPQKTTPRWLLWLAAGICIGALGL
                     LGWAILRG"
     gene            complement(9133..9765)
                     /locus_tag="PK710_00640"
     CDS             complement(9133..9765)
                     /locus_tag="PK710_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08238.1"
                     /translation="MHRWIRRAAIALTAFALIGSPTDASAETQDEIVQRLRTSRDSER
                     YEVAVDGQSIKAGGARILVQAPLAVVRTIVQDFAHYQDFMPRFKRSRVVGQSPKASQV
                     YLQVPILHGAANVWSVINFTAPVKKPDGSETIRGRMIPGQGNVRDFRATWYLVPIDAN
                     TTLLRSEILIVPTLPVPGSVVTGELGYAADMAVTSTRNRAEAQVREAAKK"
     gene            complement(10013..11089)
                     /gene="mnmA"
                     /locus_tag="PK710_00645"
     CDS             complement(10013..11089)
                     /gene="mnmA"
                     /locus_tag="PK710_00645"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOT08239.1"
                     /translation="MNDSSKSRIVIAMSGGLDSSVAAARLVDEGYEVIGVTLHLWENA
                     DPALQSRCCAPEDRRDARRVADQLGFPHYTLDRREQFGREIVEPFVDAYLTGRTPSPC
                     GPCNERIKIPALLRFAQLIDAPFVATGHYARIVGKESPRLARGVDRRKDQSYFLSTLD
                     ACTLRRLRLPLGQDTKEQVRAEAIRRGLVGSSKGESQDLCFVPDGSYARFVAERAGER
                     VRPGWVVDENGRRLARHEGIHHFTLGQRKGLGIAVGRAVFVTRIDAEQGLVVVQDAGA
                     GRVTAVTIARPTLALGVELPVHAVLQVRYRDGGAAAWLDMTDDGCVRARFDEPIRCAS
                     PGQLAVAYVDDEVVLGGTITACEH"
     gene            11394..14897
                     /locus_tag="PK710_00650"
     CDS             11394..14897
                     /locus_tag="PK710_00650"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT08240.1"
                     /translation="MVSPLWPAGALPAVQQEHQPSGLLAVDGFVCGSESRGVVSVWVP
                     TVEVASAVERHILRRSEAHQRKAISVYAQADSCWRDACMQLGVLEVPADPCEAARVLV
                     KACGRGVIVVVGQVPPGGWDHTVRVELERMASCLVVMIQWGQEQERLGSPKMIQVDGS
                     REDFDIWWEAMIQEAASRTTCESIAELDAWWSWADQQKPPVFTGDFPSLGEQTRGLLA
                     RLRLTGRSWPSRWMTILGSPRSWEELREAAAIREHGGHISLTLQAHAIEHEPTAEDYR
                     QVAEALRSKLSHEPWALARAGVLYARAGCKEQSESCLSEAMIALDDGLARRDIWEGWS
                     NALRRMPLEQRRSQALFAAELALDRDDIEASLELAHIAGGGDVSAPFEAHFALGRALL
                     ARGDVVGARVALERAYSRCATDEERGGVLAQLAEVAYTVGDLDRASRHAREAGICSTS
                     PAVRLEASNTLGKILLARSCWSEAEAHFSENENAAAAAGLYLGVVRARVNRAIALLSR
                     GQGETARAILEQVLEDATARRDHRATAFALSNLAVLAIDRHDYPQAIDLLEAAIDARR
                     KLGDKVGLARLITNLAELRLRLGLLKEAEHTLAFGQQALRPALPPTRVAHFALVAARI
                     RLAHGDTFEASRQIGVALANGSGSSDGAMMGECHRVEARIALEDGDVQRAARAIELAQ
                     PHASEPYARAEIAWLQAKLSQAAGQDPRQLASEALDKARQANDEDLLRDAHLLIAELH
                     RIDGRAQDARRSLEEAKRIRDHIVGALPSALQARYLARRDMHRLMAVEDAIRKGESET
                     SSDTDSVRPAADKDATRLEPCRFIGRHPTVLRLLAAVEKVAKSNAPVLVLGESGTGKE
                     LIAEALHHRSDRRSGPLVKVNCAALVDTLLLSELFGHEKGAFTGATARRRGRFEVAEG
                     GTLFLDEIGDISPKTQVALLRVLQDGTYERVGGGATLHANVRVVCATHRDLAAMVAQG
                     NFREDLFYRLSGVTLRVSPLRERLSDLNQIAQHLLEQIATERGEKVKHLSADALALLH
                     RHRWPGNVRELDNVLRAASLFSETQEISVHSVLEHIPTSIEPVSTPTSPLTVSAPSSV
                     PTVDSHHQTLRLAYDQIRTQGTSLSDLKRNIERECIRQALADSEGNITRAAALLGMKR
                     PRLSQLVKQYSLLEGLLEDPS"
     gene            14894..15214
                     /locus_tag="PK710_00655"
     CDS             14894..15214
                     /locus_tag="PK710_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08241.1"
                     /translation="MMVRKLIMGLMASAFGLTSCLVAPLDEEAQEDIASTSEAIRGVQ
                     PDSFRGNDGVDTSDPSASDPSTSRSMIGPSVSPQSAPSDPAKPQPDPWDGTQQKSSPT
                     TVNR"
     gene            complement(15242..15541)
                     /locus_tag="PK710_00660"
     CDS             complement(15242..15541)
                     /locus_tag="PK710_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08242.1"
                     /translation="MIDLEGLVEQIDLISSRYVDEITRDWDRQLQRYFVDLRKDPRPL
                     SAPYRPANTNGSILLDKYAYFATSEAVHSLGWQQVMTLIALGVVTEGDPLFRSSQ"
     gene            15628..18540
                     /locus_tag="PK710_00665"
     CDS             15628..18540
                     /locus_tag="PK710_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08243.1"
                     /translation="MRRWFLGLVALTLCEPACGPWKAPPKTSPPSPSFPSIPQYIEAP
                     RWIPDLESQRPYDIEPDGSRLFLTLQMRLRERPDGSIQKAPDLLPAGRSELTVLSLPS
                     RLGGGFLFASATTGQTQLWRAEDWVSPLEPILRIMGRYDTAVAGFDRLYLSGRRSNIA
                     TVAVDLASGKLRPLGPLPMPVGKGSMIFADAWRGVVVADLQGPLITFDAGSSWIPIAL
                     EDTVSSLALDADRILIHTQKAGNVVAIEADGRLTFETPPPPPTRETPSSAVRRHPLRL
                     AVERGIPLSSSHVLVAEGGKLLEISMQDGSIVRTIADAYPDSYTECQGVRLGTGIGFV
                     CGQRQGATTIYAVDPSYALQAVFQDPAPRAVYSSGNGVLVVRGPCPATAVPTGMQPYC
                     VRGRSGAFRQLLFRGDIGAERVVGMADGKIVVVIAPRPGAEARLVVIEGERTTTKTLY
                     LDTLTPKQRALAQNGLWMQGAIELEPGSVGVWVEGGGPVVGLRIGLDGKVEAGDIQTS
                     GRMLLSGPLGLVWRRDGKGLETTDGGRTWREVVLPFAPLTDEKGRQGCSAVGCAVEGW
                     LRLGWGKSAKTDELQEARTSAQTLYPSSHRYLPLRMVCSPTGRASPAAIPRPASATTL
                     SFPHISASRGWFAFGPMPTPPLSADQVGVAGGRDYGDFRFKSFAWGAKDADWGRVGRW
                     VVAFDDPFDPVGMPVTTMETVSPWSDITLASMYLNAVRTAVLDPAGRSAVLGWCPGRN
                     QCQIFGLTEGRTPLAFGSGAQWLPMVQSAVRSEDQWYLLSGQGGGFVDVHEVDATGNI
                     EKIGTYPRIIHQQFDRLQLVRRVYGPGIALWGTAMDGDGVSRYFALPIDTKTGETMPI
                     QDGGPTDLHGRFPPRCAPGQNGWLVDTQMPVSPRLRLRSGGAFGSSVRLRLRVSGDRI
                     CVEAMTGRATEREVGTERLPKGAGSEGVTMAVWDSQSDRKHELVCFADDEPAERDP"
     gene            18515..18808
                     /locus_tag="PK710_00670"
     CDS             18515..18808
                     /locus_tag="PK710_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08244.1"
                     /translation="MNLRNVILERHRGLPFGPENQICLYFQILICKKVISRPPNPTAS
                     TLSPMWRNERSVLIPAGQQPLWFWRKGNNPREITGSMILHSSGGTGKGMGART"
     gene            19003..20523
                     /locus_tag="PK710_00675"
     CDS             19003..20523
                     /locus_tag="PK710_00675"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOT08245.1"
                     /translation="MDSKNWDAVVVVTPTLNLTDLTAVYGPIRAAAEVDERVGKEVTM
                     VHTKQVAGGRLVIAPTGPIQRDWDDVRRYGDAAKAGVRRARDAGARRPLLLVPRVPVD
                     GPYDHALEVSLLGALEGLWEPLEARQVRGEQELEPMTHLGFAASTGAQGARIIETVSA
                     IETGRRLARDLAGTEPERMTPMAFAEHCRDAFAHTAVKVQLIDNNDEIQNSYPLLAAV
                     ARASSAVPRHRPCVLRLCYEGAGDIRETLLLAGKGVTYDTGGMDLKVGGNMAGMSRDK
                     GGAATVAGFIRTLAALAPAGRRVVAELGLVRNSIGANAYVSDEILTSHAGVRVRVGNT
                     DAEGRLVLADLLSHLRHRALEARAPILLSVATLTGHAARAVGPYTVAIENGPARKLRL
                     AAGLASLGDQWADPVELSRLRREDFDFIRPRTKADDVLQCNNAPSSATERGHQFPAAF
                     LLRASGLEQHGGDSRTPLPFVHLDLGGSACEGGDWQHGHPTAAMVTNLSARWAMDR"
     gene            complement(20579..21493)
                     /locus_tag="PK710_00680"
     CDS             complement(20579..21493)
                     /locus_tag="PK710_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT08246.1"
                     /translation="MDQPVAPRPSYVTALHHGILQSRVNTALKRLARCRLCPKHCEAN
                     RLADEKGECHTGRLAMVSSTHLHFGEESPLVGTGGSGTIFFTHCNLSCVYCQNYDISH
                     LGHGYAVTAPQLASLMLRLQHAGAHNINLVSPSHVVAQILEALLLAAQAGLRIPLVFN
                     SGGYDALPTLRLVEGIVDIYMPDAKYGDPEVGQRLSRVDDYPTVNRQAILEMHRQVGP
                     LTLDASGIATRGLLVRHLVLPASLADSRTILRFLAQHVSTTTYVHIMDEYRPCFLASD
                     HEQLARPLRRSEYEFVMQVAREEGLEPR"
     gene            21635..23383
                     /locus_tag="PK710_00685"
     CDS             21635..23383
                     /locus_tag="PK710_00685"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT08247.1"
                     /translation="MFSLLFQASGLGDLAPLSQRFLRCHHCGLPHNLTEEVCPITGKP
                     IRKERIQKAISDDSSELKPIPLVKPKGADPRFIGKIIGDKYRITELIGEGGMGTVYGA
                     EHLEIGRRVAIKVLNRAHLGRKEAVARFHQEARAAGTIGHPNICEIYDVGRLENGAPY
                     LVMERLDGRTLADRIQIEGALSFTDVIGTLKQVLSALIAAHAKGIIHRDIKPENIFLA
                     ERVGCVPIAKILDFGISKSADESDLGLTRTGMVMGTPYYMAPEQARGEHIDHRVDIYA
                     CGVMLYEILTGRRPFIAPSYNALVIQILSTTPRDPRELRPAIPDAFVSVVFKALAKHR
                     AERYDDAKAFIAALDEVAMQLEHPLMNAPVDRLSQKAHEQSMAQYPSEPPASDSIDIP
                     VHFSETGTGQHPVTLETGDVEVELDTGPRVVDDDPTMVAGRIGFEPRMAEDEVHDTIV
                     DPTGALRGELFSHLGKTPESGGPKSRAVVPDRLVESVDKPQGEIDRDVGGRYSMISSS
                     SEPSGPVWGYDRAAESKNTPRQSATRPSTAKRTRMPSDPDDVDDSAATTLFVNSEDGP
                     KDVQNTGSSSLPRRSS"
     gene            complement(23434..25401)
                     /locus_tag="PK710_00690"
     CDS             complement(23434..25401)
                     /locus_tag="PK710_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT08248.1"
                     /translation="MTFVFTAALAIALLVIAPFAAHLLRVRRAKEQPFPPAHLVPALT
                     SSARQRKKLEDRALFSIRLLAILALAVLGATPLLTCSKLSISRDGGASLAVAIVLDDS
                     MSMRARDDGTTTRFALAKQQALDLFASARQGDAIAIVLAGAPARVVLAPTMDLSLAEH
                     TLQRLEPSDRATDLEGAMALGASLLSELPQPQHRMVVLTDRCDGMPDAPPLGEGLSVP
                     TWIVSRQLSENANDCAVLYAEQRSGRVYARVACTGNQRDPHRNLVVREAGQTIAREPI
                     FPHLPQDTGTAELDVPIASSAFPDEVALEGSSDFIAENDVAPVARRSTSFTVGVVSDR
                     SLSNVETGGPPPVEQALVALHTGATIHPMTVMPDTVEELSKLTILVIDDPAGFTPETR
                     ESLHGWVERGGVALVGLGPRSARAPLGATLEPLVSGIPHWAEDAPDSVNERDCLHFGP
                     SGPSLLRLEAKGRAHFPFDGPTEPTAILRWSDNLPLIVERTLGRGTITVVGLPFSPQW
                     SDLPFRPAFLALLKQLVDDVGLRAGSKRIDVGQSWVIDSESVEVRTKSGPYPVAHSQG
                     KTRITPHEAGRYDLRIAQEVDSRIASIPAHEVDLRPRRITETNQENRLGTTSGTIDIS
                     RWIALFLLFLLAVELGLRARNARLLARDSAR"
     gene            complement(25398..26315)
                     /locus_tag="PK710_00695"
     CDS             complement(25398..26315)
                     /locus_tag="PK710_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOT08249.1"
                     /translation="MHPSERHNQAIDWGRLAPLRLRAKSVADGVFAGVHRSRRHGPGV
                     EFGGHRAYVPGDDLRWIDRHALMRHDQLLVRLFETETDRGVHMLLDATASMSFRGAHA
                     DSAKVAFAALIVAALTRVVLSAGDPVSLGWISGVGSRPVAPSASGDAFDRIVQALEGL
                     QATGDAMHDLRAFDRVIQSIARRARRGACVILLSDLLDLPDGAAERFAALGSQGRRLV
                     VVQVLDHEELTLPYTGTVRLASLEGNTVLETNPDVVRERYLEALDQWTERFVQPLANR
                     AGRWLRVDTQQDPVDVVIRVLHAIAEVPP"
     gene            complement(26302..>27087)
                     /locus_tag="PK710_00700"
     CDS             complement(26302..>27087)
                     /locus_tag="PK710_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT08250.1"
                     /translation="VAAERERRLAALKSEFVANVSHELKTPLSLVRMFSELLSTGRVS
                     DEAKRQKYLSIIVRESERLSALIENVLDFARLERGKVAYQFEYADVVEVVSRAVEAFR
                     HRAEREGMVLDMQVESNMPRTVLDEQALELALVNLLDNAMKYAKDGKLIRVEVRLRSA
                     KTIEIRVTDQGPGVPIDEQRRIFDRFVRGKGARDKQVRGSGIGLALVQHIASNHGGQV
                     WVESTVGKGSSFLLTLPVRSQVSPSRAAFAQAPPNEAHRDASE"
BASE COUNT         5702 a         7919 c         7886 g         5580 t
ORIGIN      
        1 agcctttcga acaacccccc gtgcaagctc gagcttccat cgaggtttcc gccgcttgct
       61 gccaccccca cacccagcac ccctcgttcg cggacatacg gaacaagaag cctcacccca
      121 atagccattc cgacaaccac ccgctcatgt tctcgactcc ctgccgcacg tcgtgctcat
      181 ccaataccag ccgctccgaa atgtcaattt ctgaaagaaa ttcatgagct tgcggattgt
      241 cgaaccaatc gtcgtcgaat cgctccacca actcacgcag ccgtgtcatc gcatgcaagg
      301 cgccgcatag cctcgacgcc gctcgaggct cgtaacgagg aaccaccccc gccaacgtct
      361 ccggaatact cgcatgcatg gctttcgaca cctctgtgac atgcgcttcc accatggctt
      421 gcggtgatgt catcgtggcg acaatcgcct gcgccgcatt catccgaacc gatatcaaaa
      481 gcgatagccc aagcgaccgc gcttgtattc gcgcagcatc acgccccaat cccatgcgac
      541 gcttcaagaa aggcacgctc gtcaacaagg aagccaataa cgcaccgtac gaagcggccg
      601 ccgcatcgaa gggccgatgg accatactga atggcccttg ggaacgcatg gcccatcctc
      661 gatggatggc accgccaaat cgcgccaggg cacgggcgaa agatgcgcca caaatcggat
      721 cgcacaacgg tccaagctcg agatgaattc cttgcaggct cttccacccg ccaaacatcc
      781 agcgaaacca acgaggcgtt agcgccgcag gccagcccga ccgcgcatcc atcgccgccg
      841 caaccctcac tacctcttgc cagctctccg cttttatccc cgcagccatc gcgtcatccg
      901 tgcccgccat gactcgttcg gctacctgcc tcacacccat gtctcgcaag ggcacttcga
      961 gccactccaa cccctccgtt cccaaatgat acgcagcccc atggcgacga atcgcccact
     1021 tcaacgcgct ttccgatgcc tccgcgctcg cttgctccag tccacgcgcc acgttcgctc
     1081 gtcgccccaa atctcggctc gccaacatct cgagcaccaa cgaccgaaga ctccacgcta
     1141 cctcccccag cccatccacc cgatgatcaa aagagcgtcg caacacttcc accgccgctc
     1201 gctcttccca cgtcacccgt tcgatcgtca aatacgcttg ccaccgaagc atagaatccg
     1261 cgttcggtac ctctttcggg tatcccgcca tctgctcgaa acgttctcgg gtcgtcaggg
     1321 agcgatacgc aaccaaaggc cctatatccc ccgatctcca gccgcccaac ctgcgagaaa
     1381 ccaaccccaa tcgatactcc gtaaacgctt gctcgatccg ttcttcccat cgtcccgggt
     1441 ctgtcatcat gacctgtcct ctgacacgcc ttgctcaatc gggaaagccc cagaatgctc
     1501 ctttcaccac ccctcgacgg cacgccgaac caacgctaga ttgcgctttg cccatgaaca
     1561 tcgacgacga gctggaaaag gccatccagc agaaagaaca aggctccgcg cgccgtcccc
     1621 gacgagacgc tcccacaccc aaacgaaatc tcggattgct tattgggttg atcgtcatcg
     1681 ccgtggtcgt tcttgccttg ctacccttca ccttcaaagg gtccgcggtc tgggctacca
     1741 ccgtggacca actcgtttcc gaacccgact tgaaaggaag acgtgtccgc gtcgaaggca
     1801 tcctcgtcaa aggctccctc atgaagcgcg attctccctg cgaataccgc tttcagctcc
     1861 gaaccaacga ggccactgtc caggttcgct acccgcaatg cgtcattccc gactcgttgc
     1921 aagaccgccc cgaagccgac atcaccgtca ccgccgaagg aacattggga caagacaacg
     1981 tattcgttgc ctcccaggtc cttgccaaat gcccttccaa gtacgaacaa aaggacggaa
     2041 aaacaatatc ttcccaccaa tcctcgagtc gttgacccca ccgtacccac ggccttttag
     2101 catccttgct ttgccaacga tcccgcgcgc gcgcgacgaa acggtcgaac cgatgggggt
     2161 atccgcaagg acttcgctag ggccaaatga ggctcgaagg cgatcgcgac aagaaatcgg
     2221 ggatggggat aaaaccgcgc ggggcttcga acgcttcagg acgcctgcac cggagagttg
     2281 cggtaggcaa ggggtgtctc gggaacgggg tatcccgccg caccgaaggt ttccacaatc
     2341 gccttgtgag tatcgaaata cacctgccaa taatgatccg tatgcgtata agggcgcaca
     2401 caaagcttgg gaccttccgg cgtgaactcg agaatctcga tatccggagc gggatctttg
     2461 gagacgttcg gaatggcggc gatcactggc ttcaaccgtt caatcgcgtc caatggattg
     2521 acactgttgg ccaccttggc cacgcaatcc acccgtcgaa acggtaacgt gctgtaatta
     2581 tggatgttat ctccgaaaat cttaccgttc cccaccaccg taaacacgtt gtccggcgtc
     2641 accaacgtgg tggcgaataa tcccaattcc gtcactgtcc ccgtcactcc ccccgcggaa
     2701 acgaaatccc ccaccttata aggtcgcaag acttgcatga acacgccagc ggcgaagttg
     2761 ccgagcattc cactccaggc cgcgccaatg gccagccctg cccccgcgag caacgccgcg
     2821 aaagacgtcg tcttgactcc gaagatgtcg agaatcgcaa ggatcagaac gatgttgagt
     2881 agaacaatcg taatggattt caagtagttg gcgagtgtct catcgatttt tccaccacgt
     2941 ctcaatagct tgccgagcaa ggttacggaa aacgtgatca gccaacgccc caccacccat
     3001 gcgatgatcg ctgtcagcag tttgagcccg aaatccccgc cttgtgtcac taagaaattc
     3061 cagatatccg ctgtattcat gatgcacctc gctgccaatg gcctatgcgc cttcccaagc
     3121 cggtaagcgt ttgattggcg tgggagccgt cgagtcgatc aatgattcag caccaacgga
     3181 cgccgtgagc atgacccttg agagcgctgg tggtagaatc accggaagtg caaagatggt
     3241 tgatggctat gcacctcgga ggcattggat gttcgacctg acggattggt ggcgcttttc
     3301 cacaaaaacc atgagatttt ccggctggaa gcctggattt cgtcggaaaa cgaccaacgc
     3361 acttctcctc gggggagcca tgggtgtggc gatcggtatg gtcgcctccg aagcccgagc
     3421 caaacgttac accaaaccag ctccgcccat gagcagctat attcaaaact atgggtttcg
     3481 ctccccatcg aacccaagcg ccaacctcaa cgcgcacaac ccacaagccc attctcagct
     3541 cgaagcctac ttgctcgaag gggcgtgggc ggcagagctg accggtgatc ctgtcatggt
     3601 agcgtggttt gccgcggccg atgcctactt ggccccatgg cgcatgagca actgcggaga
     3661 ctattcttgg gacatcgatt tcgactgccc cgtcctgcca tggggctcaa atatatggca
     3721 agtcggcctc gccgtgcagg tcagcgatag ctattcacga ggacaaaaag cgttttccgc
     3781 cacgcacccc aacgaaacgg aagcccaggt gggaaacgag gtcctgaaaa tcgccgctca
     3841 agaaaaaacg tttcccacgg gcctgtccat cgccaccatg gtctccagcc ccggcggcat
     3901 caccgcggga atgcataata gctactggtt ttccgtgctc cttcgcgaca tggccatgag
     3961 cgcctatctc gaatcccccg ccgcaacagc atgggaatgc tacaaaagca actcttatcc
     4021 tggctggtgc gaatattacc gcaaatccgc caactggcaa cactacagcg atgtcctttc
     4081 catggtaatc gtgtcctgga accagattca ggaggattgg aaaaaagcgc tggtcgacga
     4141 tgtcatcatc gatgacctgg accccggttt caaagcaccc cccggagcca aattcctcgg
     4201 ggaaaaggga tggagaggcc atttcacatg gcgcgaagct tcgagttctt cccccgtttc
     4261 cgcttcctgg acagccaacc tcccggtagc gggaacctgg accgtcagcg ccttcatccc
     4321 ctattcggac aaggcaaccg tcacaaaagg gaaggtgacc gtcgaagccc aggacggctc
     4381 ccacgaagct actttggatg agtctcaaat cggcggacga ttcgtcgaac tcggcacctg
     4441 gcccttcgaa tcgaaagctg tcgtacgcat gacgaatcag ggcacgagta cggagtgtgt
     4501 cggatgggac gccgttcgct tccacctcgt cagcaaagca agtcccgatg caggaactcc
     4561 cgatgggtcc gcgggaagtg gcggcagtgg cggaagcagc ggtgaagacg ctcatgccga
     4621 cgtttccggc gacgagacca ccgaggcttc cgcagacagc tcccacggcg aaaccgggat
     4681 cgtctccgat gctaacgccg accactccga taccgatgcg cgcgatagtg gcatgtccga
     4741 cgcccacccc gaagcaggga atcatcccgc ttcgtggcct tctgatcagg aagccgatgg
     4801 ctgcgcttgc cttgtcgcgc gaaatcaatc acgcagatac gctcatcttg ccgtgcttgc
     4861 cctggccgct atcggtttcg gacttcgacg ccatcgacgc gggcattcgc tccgttcgta
     4921 aacgcgttca tcagccggga ttccaggtcc tggcatcgtc gtcgctcacc aaaatctcga
     4981 acgatgtcga gcaatgagaa gcgtccgggc agctttcccg cgcaaccatc cccaccatac
     5041 gtttgcccac gcccacgaag gcaaggccgg aaatcgtaga accatccgtt cccacttcgg
     5101 caagaaaacg aggtgccacg aactcattat ccgttttgtt gttgatggta taaagcgcgt
     5161 atccccgcgc aacccaccca aggacaaccg caccggtggc agcgctcgtg gccgcggttt
     5221 ggttatacaa cgcaatatcg gtccccgcgg tcgtgtggat cttgccccct tcgagttctc
     5281 ctcctcgaat cgttagtaaa ttcgcgaaga tacgatcctt caggaaggga agcgccaccg
     5341 tcgccacgcc aggaccacac ccaatatggg agccttcgtc agccagggct gtcatctgca
     5401 cgagttggag aaaattgggt tgcatgccga tcaagaaact gcccgtggtg gtcgttcccc
     5461 aatagactcc atcgctacct acacaacgga accacggcga gccacgcaac gctggttcca
     5521 ccggcttgac caccaccgac gacgccatca taccgatggg ctctcctggc ggtggaatcg
     5581 aaaacgaagc cattttttgc gctggtaggc ttccttcccc cacgagcgcc atgggttcgg
     5641 ccccaggaac cagcccagcc aacccatatc ctgatagcga cagcacggtc accggctgat
     5701 tcgccacggc gtaagcgatg ccgtcctccc gcttatgatc gtcatcgata ccaagagccg
     5761 cgcgcgcgac tcgacgttgc tcagccaacg tgatcagcgc ttgcacatcc ttgtccgcaa
     5821 agtttttttc ccactcctcg cgctcttttt tctctgcttt tttttcttcg ttggttttct
     5881 cgacgatgcg ccatacggga aacgccacgg taccttgtga aagcacgacg gcttcccgct
     5941 caccgcctgg ggcgcgtttc caatcctcgg gcgggggtgg aaaggaggtt ggcgctggca
     6001 gttgtccttc gacgatcttg cctacctgga cttgtgcttg ttctttttga ccggaagcga
     6061 tcgagaacca gcgttcgccc ccgggggcat cgaaagcgcg aaattccatg tcttttttgc
     6121 ccgcgaagcc aacaggtccc cgagctgatt tccacgtggt accatcatcg gtccaggcca
     6181 ctgccatctc gccctgttca ttgacggcgt gcagcatcag accttttccc gaagggatga
     6241 gccgccagtg tgccggattg accttgcctg aaatcgtggc tatggcgcgg cctttggcga
     6301 ctcgatcggg aacgttcgga gctttgcgca agcgggtctc gcctgccttt tgaatttccg
     6361 catcgcttgc cccttcgctt ttgtacatgc ctcgggcggc atcaagcatc tcgaacgaca
     6421 gttcgcccgc ttgaagaacg ctgccgtatt tttccttgcg caaaaacacc tggtccgacg
     6481 acttatccaa gggaagcgcg cgaaacgctt catacgcttt gtcgaatcgc tgcttgaacg
     6541 cgactccatc ctgccccttg gctcccgaag cgttgaacca cgtcgactcg taggcgtccc
     6601 acttctcctt gacgacatcg agcgccagaa ggcacggttt ggccgtgttg atatccggca
     6661 gatcgagcac catcctgcgc tcaaaacctt gatggacctg cgttcgttgc agttccacat
     6721 catcgccggc catgcaacgc gccatatccg ccgcagcgct cagcaattcg ccagcccgtt
     6781 gctccgcgcg actcgcggac acacgccagg taatcaccaa cgaaaggagc gccaccccac
     6841 ccgccgcgcc caccacgatc atcttttgct gtttcttatc catcggttct cacaaggtcg
     6901 gccatgctca tagcggccga ggtcattcgt caagacaact ttgcatccat cctcccggag
     6961 atacaataaa acgtaataat atccaactgt tatgatgtta ttgcggagcc tttccccaag
     7021 gcggacaacc aggatccgtt catgatatcg actctttcat ccttccaggg cacccaagcc
     7081 gcgcggatgg cttgcactct gcctgcgtcc gtgcttggat tttccaccgt ccttccccaa
     7141 caaggggtgg aacaatctaa gactcttccc atcccgtccc cgcatcggca cgcggcggca
     7201 cggaatgact gcgatcgtgc aatcgaacac aacatggtct gactcgagat cgaacgtcgg
     7261 tcgcgcgtag cgcgtttgca cgcggagact aagctccagc gaatcatcac accgcaacga
     7321 ggacgaagtg atgcgagaaa gaaagaaaaa atcatggcgt gagatcgatc agatgcgcga
     7381 tgggtcggcg catcgccagc gcgaacgaga ccaggaacgc gaatccatcg cgcgagccaa
     7441 tgccgccacc aaacagtatc gaaacgcatt ggaagccctg ttttctccaa aagccgcgga
     7501 agaaaatcaa cccgtcctac ccaaaaccgc cgctcgtatc gtcctgcctc cggtccccga
     7561 agccgacccc aaccatgcgg aacgacgtcg tttgcttggg aagtttctcg cggccaccgg
     7621 tcctgcatcc atctcgaaag cagcagatat gtttttcgcc gcgggattct ccttaccgga
     7681 agaccaggaa actcacgtgc aaatgctcga gcatctggat gaaaagcggg tccgggactc
     7741 catcgccaag ctagtgacca tcttcgcggg ggaagttcct cgacgacgcc ccattgtcga
     7801 gcagcggcta cgccgcatcg aagaacaagc cgatgagcac gatacccgtg tggctgctgc
     7861 atcgctgcgc agaatgcttc atggtcgtgg tactggctcc ccttcctcta cgagcatctg
     7921 atgaacaaat ccgaattcga atcccgtttc cgagatctcg ttcgaaccta cgcttcgctg
     7981 cgcgacaacc agggatgcat tgactgcatc ggctgcgaac gttgctcttc ctgcacgttc
     8041 tgccgcaatt ccaaaggtct cgttcgctgc cacttctgtg tcgcctgcga agactgctcc
     8101 gattgctctc atggccgtca gtgcgtgggc tgtctcgctt gccaccactg tgtcggatgt
     8161 gagcgctgcg tgcaaagcgc ctatctcatc cgatgcgtcg gcctgacttc ctgcaactac
     8221 tgcttcggtt gtgtcggcct ttcccacaag gattatcaca tcctcaacga accttatgac
     8281 cgcaaaacct attatgaaat cacccaacgc atctcccgcg agcttgcgct gacgaaagcc
     8341 tgaacccatg cgtgcggtgg tgcagcgggt tgcttccgcc agagtcgaag tcgacggctg
     8401 cgtggtagga caaatcggtg caggcctcgt cgcattcgtg ggcgcgggcc gcgatgatac
     8461 ggacaacgat gccgatctgc ttgcttcaaa aatttccggc ttgcgggttt ttgaagatga
     8521 tttgggaaaa atgtccctcg gcgtcggaca agtaaacggc gccattcttg ccatcagcca
     8581 gtttacggta ttcggggatg tgcgaagggg acgacggcct agtttcgacg atgcgatgga
     8641 accggtgcag gcccaacgtc ttttcgaccg tttcgtcacc cgcgttcgcg acacgggaat
     8701 tgccgtggaa accggacgtt ttcgagccat gatgcgggtg ctggtcgaca atgacggccc
     8761 ggtgaccatt ctcatcgatt cacgaaaagt attctgacat gaacgacgag aacaacgacc
     8821 cgctcgacgt tctttggaac catgtcttga cgcaatggga caatcccaaa gcccacgaaa
     8881 gcctgatgca gttgggatgg caacgagaac aattgggcca ggtggccgcg tggtatcgac
     8941 aacagctcga taacccagaa cgtcaaccca ccgcccaggc catgctccag tcgctgaccg
     9001 tgctcgccac ccagcaattg gaaaactgcc gttcgccgca aaaaaccaca ccacgttggt
     9061 tgctatggct tgcggcagga atttgtatcg gtgcgttggg gttgctcggc tgggccatcc
     9121 tgcggggatg acctactttt tcgcagcttc gcgcacttgc gcttcggctc ggttgcgcgt
     9181 ggatgtcaca gccatatcgg cggcgtatcc tagttctccc gtcaccacgg aacccggcac
     9241 cggaagcgtg ggaacaatga gaatctcgga ccgcaacaac gtggtattcg catcgatggg
     9301 cacgagatac catgtcgcgc ggaagtcccg aacgttaccc tgaccgggaa tcatcctgcc
     9361 tcggatggtt tccgaaccat ccggtttttt caccggcgcg gtgaagttga tgacactcca
     9421 tacgttcgcg gcgccgtgca aaatcggtac ttgcaagtac acctgcgagg ctttcgggct
     9481 ttgtcccacg acgcgactgc gcttgaagcg tggcatgaag tcttgataat gcgcgaagtc
     9541 ctgaacgatg gttcgcacca ccgcaagcgg tgcctgaacc aaaatacgcg ccccaccagc
     9601 cttgatgctc tgaccgtcga ccgcaacctc atatcgctcg gaatcacgcg acgttcgaag
     9661 acgttgaacg atttcgtctt gtgtctctgc ggaagcatcg gtgggcgaac cgatgagggc
     9721 aaaagccgtg agggcaatgg cagctcttcg aatccaacgg tgcatggtgc tcatcctctg
     9781 cgatagatta tgatctgaaa cgggtatcgc caagctttcc aaaagctttg atgcatgaga
     9841 tgtgcctgcc tggttctctc cgagttttcc tacgatgcac ggacggtctt tcggggtatg
     9901 gacgacagaa aggccaatta tgttcagata tttcgaacca ccgtcggccc gaaccgatcg
     9961 ccgacgagcg ggcggccccg atcaaccagt gtcgcgaaaa aaccacaggc cctcagtgtt
    10021 cacaagccgt gatcgtccca ccgagcacca cttcatcatc cacgtacgcc accgcaagct
    10081 gccccggact cgcacaacga atgggctcgt cgaaccgggc acgcacgcaa ccgtcatcgg
    10141 tcatgtcgag ccatgccgct gccccgccat cccggtacct cacctgcaac accgcgtgaa
    10201 cgggaagctc cactcccagc gccagggtag gtcgagcgat ggtcaccgcc gtcacacggc
    10261 ccgcgcccgc atcttgcacc accaccaacc cctgctccgc atcgattcgt gtgacgaaca
    10321 cggcacgccc cacggcaatc ccaagccctt tgcgttgacc caacgtaaag tggtgaattc
    10381 cctcgtgacg cgccaatcgt cgaccgttct catccacgac ccaccccgga cgaacccgct
    10441 caccagctcg ctccgccacg aatcgcgcat aagaaccgtc cggcacgaaa cataaatcct
    10501 ggctttctcc tttgctcgaa cccaccaatc cccgacgaat cgcctcggcc cgtacttgct
    10561 ctttggtgtc ttgcccaaga ggaagtcgta gtcgacgcaa cgtgcaagca tcgagggtac
    10621 tcaaaaaata gctctggtct ttgcgccgat cgactccccg ggccaaacgc ggtgactctt
    10681 tgcccacaat cctagcgtaa tgtcccgtcg ccacgaacgg cgcgtcgatg agttgggcga
    10741 accggagcag tgccgggatt ttgattcgct cgttgcacgg accacaaggg ctgggcgttc
    10801 ggcctgtcag gtaggcgtcg acaaacggct cgacgatttc cctgccgaat tgctctctgc
    10861 ggtcgagggt atagtgtgga aagccaagtt gatcagccac acgacgcgcg tcgcgacggt
    10921 cctcgggcgc gcagcagcgg ctttgcagcg ccggatccgc gttttcccat aaatgaaggg
    10981 ttaccccgat gacttcgtaa ccctcgtcaa ccaaacgtgc cgcggcgaca gacgaatcca
    11041 acccgccgct catggcgatg acgatacggg acttggagga atcgttcacg gctggtacgg
    11101 caatctcgga gattgggtcg ggttcatagg cgtcgggagg ggattcgaca aggggatgtc
    11161 ccctgatggg tggacagggt acggcttcga aaacctgagt cctgctgttg taactaattg
    11221 attttattat atttgcactt attccggcag agtggggctc tgacccacat cccgagcccc
    11281 gcacttcgag ttccgaagct tgcgccccgt gggttattgg gcgttgggtt tctgggtcgt
    11341 cgagcgaatt cctcggttgc acgactccgg cggggagcgt aggctacgtt atcgtggtat
    11401 cgcccctttg gcccgcgggt gctctgcctg ccgtgcagca ggagcatcaa ccgtctggtc
    11461 tgttggccgt cgacggtttc gtgtgcggtt cggaaagccg gggtgtggta tccgtgtggg
    11521 ttcccacggt agaggtagcc tcggcggtcg agcgtcatat tcttcgtagg agtgaggcgc
    11581 accagcgaaa ggcaatcagt gtatacgcgc aggcggactc gtgttggcgg gatgcgtgta
    11641 tgcagcttgg agtgctcgag gtgcccgcgg atccctgtga agcggcgcga gttttggtga
    11701 aagcatgcgg ccgtggcgtg attgtggtgg tggggcaagt gccgcccgga ggttgggatc
    11761 atacggttcg ggtcgagttg gagcgcatgg cttcttgtct cgtggtgatg atccaatggg
    11821 gacaagagca ggaacgattg ggatccccga agatgatcca agtcgatggg tctcgggaag
    11881 atttcgatat ttggtgggaa gcgatgatcc aggaggcagc atcgcggacc acgtgtgagt
    11941 ccatcgccga actcgacgct tggtggtcct gggcggatca gcagaaaccc cctgttttta
    12001 caggagattt tccatcgttg ggcgagcaga cccgagggct tctcgcgcgg cttcggttga
    12061 cgggacgatc gtggccttcg cgatggatga ctattcttgg ttcaccacga tcgtgggagg
    12121 aattgcgcga agccgcggcg atacgggagc atggtggtca tatcagtctc acgctccagg
    12181 cgcatgcgat cgagcacgag cctacagcag aggattatcg acaggtggcc gaggcccttc
    12241 ggtccaagct ttcgcacgag ccctgggcat tggcgagggc gggcgtgtta tacgcgcgcg
    12301 cggggtgcaa agagcaaagc gaaagctgtt tatccgaagc gatgatcgcg ctcgatgatg
    12361 ggttggcgag gcgtgatatt tgggaaggct ggagcaatgc gttgcgtcgc atgcccctcg
    12421 agcagcgtcg ttcgcaagcc ttgttcgcgg cggagttggc gctcgatcga gatgatatcg
    12481 aggcttcgtt ggagcttgcg catattgctg gcggtggtga tgtatcggcg ccattcgaag
    12541 ctcatttcgc gctagggcga gcgttgctgg cgcgggggga tgtggtaggg gcgcgtgtgg
    12601 cattggagcg ggcgtattct cgatgtgcga cggatgaaga gcggggcggg gttctcgcac
    12661 aactggcgga agtggcttat acggtgggtg atctcgatcg agcgagccgt catgcacggg
    12721 aagcgggtat ttgctcgaca tcgcctgcgg ttcgcttgga agcgagcaat acgttaggaa
    12781 agatattgtt ggctcgatcg tgttggtctg aggcggaggc gcatttttcg gaaaatgaaa
    12841 atgcggcagc ggcggccggt ttgtatctcg gcgtcgtccg ggcgcgggtg aaccgggcca
    12901 tcgctttgct ttcccgaggc caaggagaaa cggctcgagc catcctcgaa caagtgctcg
    12961 aagatgccac cgctcgccga gaccatcgag ccaccgcttt cgccttgtcg aacctcgcgg
    13021 tgctggcgat cgaccgccac gattatccgc aagccatcga tcttctcgaa gcggccatcg
    13081 acgcacgccg taagcttggg gataaagttg ggctagcacg gctcatcacc aatctggcgg
    13141 aattgcgtct tcggctcggg ctgctcaagg aagcagaaca tacgctcgct ttcggacagc
    13201 aagccttgcg tcccgccctt ccccccacgc gagtggctca ttttgccctc gtggcggctc
    13261 gaatccggct cgcccatggc gatacgttcg aagccagtcg gcaaatcggg gtcgcccttg
    13321 cgaatggttc ggggtccagc gatggggcca tgatgggcga gtgccaccgc gtagaagcaa
    13381 gaattgcttt ggaagacggt gatgtgcagc gagctgcgcg tgcgatcgag cttgcgcaac
    13441 cgcatgccag cgagccgtat gcgcgcgcgg aaatcgcgtg gctacaggca aagctttcgc
    13501 aagcggcagg acaggatcca aggcaacttg caagcgaggc cctcgataag gccaggcaag
    13561 ccaacgacga ggacttgttg cgtgatgctc atcttttgat tgcggagcta catcgcatcg
    13621 atggtcgagc gcaggatgcg cgacggagcc ttgaggaagc caaacggatt cgtgatcata
    13681 tcgttggtgc cctgccttcg gctttgcaag cgcgctacct ggcgcgacgc gacatgcatc
    13741 ggctcatggc ggttgaagat gcgatccgaa aaggggaatc ggaaacgtca tcggatacgg
    13801 actccgttcg accggctgcc gacaaagacg ctacgcgatt ggagccctgt cgtttcatcg
    13861 gaaggcatcc caccgtgctg cgcttgcttg cagccgtgga aaaagtagcc aaatccaatg
    13921 cccccgtgtt ggtattgggc gagagcggca ccggcaaaga gctgattgca gaggctttgc
    13981 accatcgaag cgatcgtcgt tccggcccct tggtcaaagt caattgtgcc gccctcgtcg
    14041 atacgcttct gctatcggaa ctattcggcc atgaaaaagg ggcttttacg ggtgcaacgg
    14101 cacgacgtcg tgggcgcttc gaagtggcgg aaggcggaac gctgtttttg gacgaaatcg
    14161 gggatatctc gccgaaaacc caggtggctc ttctgcgggt gttgcaagat ggcacctacg
    14221 aacgcgtggg aggaggagcc acgttacacg ccaacgtccg tgtggtttgc gctacccatc
    14281 gcgacttggc tgccatggtg gcgcaaggca atttccggga agatttgttc tatcggctca
    14341 gtggcgtgac actgcgcgtg tcgccccttc gggagcgctt gtccgacttg aaccagatcg
    14401 cccagcatct gcttgaacaa atcgccaccg aacgaggcga aaaggttaaa cacctttcgg
    14461 ccgatgcctt ggctctgctg catcgtcacc gatggcccgg caacgtgcgt gagttggaca
    14521 acgtattgcg cgcggcaagc ttgttctcgg aaacacagga aatctcagtc cattccgtgc
    14581 tcgaacacat ccccacgtcc atcgagcccg tatcgactcc cacttcaccg ttgacggtat
    14641 ccgcgccatc ctcggttccc accgtcgata gccatcacca aacattgcgt cttgcttatg
    14701 accagatccg cacgcaaggc acgagcctgt cggatctcaa acggaatatc gaacgcgagt
    14761 gcatccgaca agcgctggct gattcggaag gaaacatcac gcgcgccgct gccctgctgg
    14821 gaatgaagcg gcctcggttg tctcaactcg tcaaacaata ctccctgctg gaagggttgt
    14881 tggaggatcc atcatgatgg ttcggaaact catcatgggg ctgatggcat ccgcattcgg
    14941 cctgacaagt tgcctggtgg cgcctctcga cgaagaagcg caagaggata tcgcttcgac
    15001 ctccgaagcc atccgtggtg tccaaccaga ttccttccga ggaaacgatg gtgtcgatac
    15061 ttccgaccct tcggcttcgg atccttcgac ttcccgttca atgataggcc cgagcgtttc
    15121 accacaatcc gcgccctcgg acccggcaaa acctcaaccg gatccatggg acggtaccca
    15181 gcaaaagtcc tccccgacca cagtcaaccg ctgaatttta ttgcactttt caaattcccc
    15241 atcactgaga agaacggaat agcggatctc cttccgtcac cacacccagc gcaatcaaag
    15301 tcatgacctg ctgccacccc agcgagtgga ccgcttcgga agttgcgaag taggcatact
    15361 tgtcgagcaa aatggagccg ttggtattgg ccggacgata aggagcggaa agggggcggg
    15421 gatctttgcg cagatccaca aaatatcgct ggagctgccg atcccaatcc cgtgtgattt
    15481 catccacata tcgtgatgaa atcaaatcga tttgctcaac caacccttcc agatcaatca
    15541 cgagccatcc ttacgccatt gcgataacat gcgattcgag gtccggcaat gagtacacca
    15601 agcttgggaa cgcgctacca tgcgatcatg cgtcgttggt tccttggcct ggttgcgttg
    15661 acgttatgcg aaccggcctg tggtccatgg aaagcgccgc ccaaaaccag cccgcccagc
    15721 ccttctttcc catcgattcc ccaatatatc gaagcgcctc gatggattcc ggatctcgaa
    15781 tcccagcgcc cctatgacat cgagcccgat ggttcccgtc tttttttgac gttgcagatg
    15841 cgtctgcggg aacgtcctga tggttcgatt caaaaagctc ccgacctgct gccggcaggg
    15901 cgctccgaat tgacggtttt gtcgttaccg tcacgtctcg gaggtgggtt cttatttgcc
    15961 agcgccacga ccgggcagac gcagctttgg cgcgccgaag actgggtctc tccgctcgaa
    16021 cccatcttac ggatcatggg caggtacgat acggccgtag cgggcttcga ccgtctgtat
    16081 ctcagcggtc gtagaagcaa cattgcgacg gtagcggtcg atttggcatc cggtaagctt
    16141 cgtccattgg ggccgttgcc gatgccggtg ggcaaaggat cgatgatttt tgccgacgcc
    16201 tggagggggg ttgtcgtggc ggacctgcaa ggcccactga tcacgttcga cgccggttcc
    16261 agttggatcc ccattgctct cgaagacacg gtaagttccc tggcgctgga tgccgatcgt
    16321 atcctgattc acacacaaaa agccggaaat gtcgtagcca tcgaggccga tggccggttg
    16381 acgttcgaaa cgccgcctcc accgcccacg cgggaaaccc cttcgtccgc ggtgcgtcga
    16441 caccctcttc ggctcgcggt cgagcgcgga atccccctgt catcgtcgca tgtcctcgtg
    16501 gccgaagggg gaaagctgct cgaaatatcc atgcaggatg gttccatcgt tcgcaccatc
    16561 gccgacgcct atccggactc ttataccgaa tgccagggcg tgcgtctcgg aacggggata
    16621 gggtttgtat gcggccaacg gcaaggagca acgactatct acgcggtgga tccgtcgtac
    16681 gcgctgcagg cggtattcca ggatcccgcg ccgagggctg tctattcgag tggcaatggg
    16741 gtgttggtgg tcaggggtcc gtgtcccgct accgcggtgc cgacggggat gcagccctat
    16801 tgtgtgcggg gacggtcggg agcatttcga cagctactat ttcgagggga tattggagcg
    16861 gagcgcgtgg tgggaatggc cgatgggaaa atcgtcgttg tcattgcgcc acgtccgggg
    16921 gcggaagctc gtcttgtcgt catcgaaggc gagcggacca cgacaaaaac cttatatctc
    16981 gacacgttga cgcccaaaca gcgcgcgctg gcgcaaaacg ggctttggat gcaaggggcc
    17041 atcgaactcg aaccgggctc ggtgggcgtg tgggtggaag gaggcggacc ggttgtcgga
    17101 ttgcggatcg ggttggatgg aaaggtagaa gcaggggaca tacaaacatc gggcagaatg
    17161 cttctatcgg gtcctctggg gttggtctgg cggcgggatg gcaaggggtt ggaaacaacg
    17221 gatggtggac ggacgtggcg agaggttgtt ttgcctttcg ccccattgac ggacgaaaag
    17281 gggcggcaag ggtgttctgc cgtgggctgc gcggtggagg gatggttgcg cctcggttgg
    17341 ggtaagtccg cgaaaacaga tgaattacag gaagcccgga cgtccgctca gaccctttac
    17401 ccctcctccc accggtatct tcccttgcgg atggtgtgca gcccaacagg acgcgcttcg
    17461 cccgcggcga taccacggcc agcttctgcc acaaccctat cttttcccca tatatctgct
    17521 tcccgaggct ggttcgcttt tggacccatg cccaccccgc ctctgagcgc ggatcaggta
    17581 ggcgtggccg gggggcgtga ttatggcgat ttccggttca agtcctttgc ttggggggca
    17641 aaagacgccg attgggggcg tgtgggacga tgggtggttg cttttgacga tccgttcgat
    17701 ccggtgggaa tgccggtgac cacgatggaa acggtatcgc catggtccga tatcacgttg
    17761 gcttcgatgt atctgaacgc cgttcgaacc gcggtgttgg atcccgcggg gcgttcggca
    17821 gtgcttggct ggtgtccggg gcgcaaccaa tgccaaattt ttggccttac ggaagggcga
    17881 actcctttgg cgtttggttc cggcgcgcaa tggcttccca tggttcaatc ggccgtgcgt
    17941 tcggaagacc aatggtactt attgagtggt caagggggtg gcttcgtcga cgtacacgaa
    18001 gtggatgcca cgggaaatat tgagaaaatt ggcacctatc cccgcatcat tcatcaacaa
    18061 ttcgacagat tacaacttgt ccgacgagtc tatgggccag ggattgcgtt atggggcacc
    18121 gccatggatg gcgatggggt gtcgaggtat tttgcgcttc cgatcgatac gaaaacgggc
    18181 gaaacgatgc cgattcagga tggtgggccc acagacttgc acggacgttt tccgccacgt
    18241 tgtgctcccg ggcaaaacgg ttggctggtg gacacgcaaa tgccggtatc gccgcgtctt
    18301 cgattgcgtt cggggggggc gtttggaagt agtgtgcggc tgcgtcttcg agttagtggc
    18361 gatcgaattt gtgtggaagc catgacagga cgggcgaccg agcgcgaagt cgggaccgag
    18421 cggcttccga aaggggcggg ctcggagggg gtcaccatgg cggtatggga ttcgcaaagc
    18481 gatcgcaagc atgagctggt ctgttttgcg gacgatgaac ctgcggaacg tgatccttga
    18541 gaggcaccgc ggccttccct ttggcccgga aaaccaaatt tgtctttatt ttcaaatact
    18601 tatttgtaaa aaagtcatct cacggccccc caatcccacg gcatcgacgc tctccccgat
    18661 gtggcgcaac gagcgatccg tcttgatccc ggcgggacaa cagcccctgt ggttctggag
    18721 gaagggcaac aatccgcgtg aaatcactgg ttcgatgatt ctgcattcca gcggcggcac
    18781 cggcaagggc atgggtgcaa ggacctgatc ggcttgtggc gcttggtgtt gcccggggtg
    18841 gcgctgagcg gttccatcga ggcgtcggcc gtttatcgtc gtgacgaggt caccgggcgg
    18901 taccaactta cgagagcgtg tactacggtg tgaattgctg gaggtagtga acaagtggcc
    18961 attcctacgt tgatcgaagt ttctgatgga tcgctaagct ctgtggactc caagaactgg
    19021 gatgcggtgg ttgtcgtaac cccgaccttg aacttgacgg acttgacggc tgtgtatggg
    19081 ccgattcgcg cggcggcgga ggtggacgaa cgcgtgggca aagaagtcac gatggttcat
    19141 acgaaacagg tggcaggagg gcgattggtg attgcgccga cagggccaat tcaacgggat
    19201 tgggacgatg tgcggcgtta tggcgatgcc gcgaaggcgg gagtgcgaag ggcgcgagat
    19261 gcgggggcca ggcgtccatt gttgttggtg ccgcgggtgc ccgtggatgg cccttatgac
    19321 cacgcgctcg aagtatcgtt gctcggcgcg ctcgagggat tgtgggagcc gttggaggca
    19381 cggcaagtgc gaggcgaaca ggaattggaa cctatgactc atcttggatt cgccgcttcg
    19441 acgggggcac agggagcacg gatcatcgaa acggtatcgg ctatcgaaac cgggcgtcgt
    19501 ttggcgcggg acttggcggg tacggagcct gagcggatga cgcccatggc ttttgcggag
    19561 cattgccgag acgcgttcgc tcacacggcg gtcaaggtgc aactaattga taacaatgat
    19621 gaaattcaaa attcctaccc actattggca gcggtggctc gggcctcttc ggcggtgcca
    19681 cggcatcggc cttgtgtgct tcgtctttgt tacgaggggg cgggggatat tcgggagacg
    19741 cttcttcttg cgggcaaagg ggttacgtac gacacggggg gcatggacct gaaagttggg
    19801 ggcaatatgg cggggatgag tcgggacaag gggggagcgg ccacggtcgc gggattcatt
    19861 cgcacgttgg ccgcgttggc ccccgcgggt cggcgcgtcg tcgctgaact cggcctggtc
    19921 cgaaatagca ttggcgcgaa cgcgtatgta tccgatgaaa tccttacgtc gcatgcggga
    19981 gtacgggttc gggtgggcaa tacggatgcg gaaggacgtc tggtattggc ggatttattg
    20041 tctcatttgc ggcacagggc cctcgaggcg cgggctccga tcctattgtc ggtagcaacg
    20101 cttacgggtc atgcggcccg agccgtgggc ccttatacgg tcgccatcga aaacggtccc
    20161 gcgcgaaagc ttcgtcttgc cgcgggtctt gccagcctgg gcgatcaatg ggcggatcct
    20221 gtcgagctat ccagattgcg acgggaggac ttcgatttca ttcgtccgag gacaaaggca
    20281 gacgatgtgc tccaatgcaa caacgcgcca tcgtcggcca cggagcgagg acatcaattt
    20341 cccgccgcgt ttttgctgcg ggcgtcgggg ctcgaacagc atgggggtga ttcgcgaacg
    20401 ccgttgcctt tcgtgcattt ggaccttggg ggcagcgcct gtgaaggagg cgattggcag
    20461 catgggcatc cgaccgcggc gatggtgacg aatctatcgg ctcgctgggc gatggatcgc
    20521 taacctaccg caagcataat ttattcaata atttccaata gatacaacaa tagcgatctc
    20581 aacggggttc gagaccttct tctctcgcga cctgcatgac gaattcgtat tccgagcgtc
    20641 gcaagggtct ggcgagttgt tcatggtctg aagcgagaaa acaagggcga tattcgtcca
    20701 tgatatggac gtaggtggtg gtcgatacgt gttgagcgag gaagcgaagg atggtgcggg
    20761 agtcggcgag actggctggc aagaccaggt gacggacgag caatcctcgg gtagcgatcc
    20821 cggaggcgtc gagtgttagg ggtcccactt ggcgatgcat ttccaggatg gcttgacgat
    20881 tgacggttgg gtaatcgtca acgcgactga gtcgttggcc gacttccggg tcgccgtatt
    20941 tggcgtcggg catgtaaata tcgacgattc cttcgaccag gcggagggta ggcagggcgt
    21001 catagccgcc ggagttgaag accaggggga tgcgcaatcc tgcttgagcg gcgagcagaa
    21061 gggcttcgag gatttgcgca acgacgtggc ttgggctgac gaggttgatg ttgtgtgcgc
    21121 cggcgtgttg caatcgaagc atgagcgagg cgagttgtgg ggcggtgacg gcgtagccgt
    21181 gaccgaggtg actgatgtcg tagttttggc aatagacgca cgaaagattg cagtgggtga
    21241 agaagatggt tccggagccg ccggttccga cgaggggaga ttcttcgccg aagtgcagat
    21301 gtgtactgct caccatggcg agtcgtcctg tatgacattc acctttttcg tcggccaaac
    21361 ggttggcttc gcagtgtttg gggcagagtc ggcagcgggc gagacgtttc aaagcggtat
    21421 tcacacggga ttggagaatg ccgtgatgaa gggctgtgac ataggaaggc cgtggggcga
    21481 ctggctgatc cattccatga ttgtgggtga caaacggggg tgatggcaga aagaaaaagg
    21541 gatggacggg caaatcgaac catttccgca aaggtgcaag acgcgactta tcgttgagcg
    21601 atggccgatc ctccgctagg cttgagaagg cgaattgttt tcgctgttgt ttcaggcttc
    21661 tggtcttgga gacctcgcac cgttgagtca gcggttttta cgttgtcatc actgcggatt
    21721 gccgcacaat ctgacggagg aagtttgtcc aatcacgggc aaacccattc gtaaggagcg
    21781 gattcaaaaa gcgatctccg acgatagttc cgaactcaag cccattccgt tggtcaaacc
    21841 caaaggcgcc gatcctcggt tcatcggaaa aattatcggc gacaaatacc ggatcacgga
    21901 actcatcggt gaagggggaa tgggaacggt atacggtgcc gaacacttgg agatcggtcg
    21961 tcgcgttgcc atcaaagtgc tcaaccgggc tcatcttggg cgcaaggagg cggtggcacg
    22021 atttcatcaa gaagcgcggg ccgctggcac gatcggccat cccaacatct gcgaaatcta
    22081 tgatgtggga aggctcgaaa acggtgctcc gtacctggtg atggagcgtc tcgatggcag
    22141 aaccctggcc gatcgcattc aaatcgaagg agccttgtcg ttcacggacg tgattggcac
    22201 gctcaagcaa gtgttatcag ccctcattgc ggcccatgcc aaagggatca ttcatcgaga
    22261 catcaaaccc gagaatatct tcctagccga gcgtgtgggc tgtgttccga tcgctaagat
    22321 cctggacttt ggcatttcca agtccgcgga tgaatcggac cttggtttga cgcgcacggg
    22381 gatggtcatg ggcaccccct attacatggc tcccgagcaa gctcgtggag agcatatcga
    22441 ccatcgtgtc gatatctacg cctgtggcgt catgctctac gaaatcctca cgggacgtcg
    22501 tcctttcatt gcgcccagct acaacgcgct ggtgattcag atcctgtcga cgacccctcg
    22561 tgatcctcgc gaattacggc ccgcgatccc ggatgctttc gtttccgtcg tgttcaaggc
    22621 gctcgccaag catcgtgccg agcgttacga cgatgccaaa gcgttcatcg ccgcgctcga
    22681 tgaagtggcg atgcagttgg agcatccttt gatgaacgca ccggtcgatc ggttgtcaca
    22741 aaaagcccac gaacaatcga tggcccagta tcccagcgag cctcctgcgt cagacagcat
    22801 cgacattccc gttcacttct ccgaaaccgg caccggacag catcccgtca cgcttgaaac
    22861 cggcgacgtg gaggttgaat tggatacggg acctcgtgtc gtggatgacg atccaacgat
    22921 ggtagcggga cggattggtt tcgagcctcg catggcagag gatgaagtcc acgacacgat
    22981 tgtcgatccg accggtgcgt tgcgaggaga gctgttttct catctcggaa aaacccccga
    23041 aagtggaggg ccgaagtctc gggcggtggt accggatcgg ttggtcgaaa gcgtcgacaa
    23101 accccaaggg gaaatcgatc gcgatgttgg tgggcgttat tcaatgattt caagtagttc
    23161 tgagccatcc gggccagtct ggggctatga tcgcgcggcg gaatcgaaaa acacgcctcg
    23221 acaatcggcg acgcgtccat cgacggcgaa acggacgcgg atgccgagtg atccggacga
    23281 tgtggacgat agcgcggcca ccacgctttt cgtcaattcc gaggacggtc cgaaagacgt
    23341 tcaaaacacc gggtcttcgt cacttccccg acgttcctcg tgagcatggg catggggagc
    23401 gaccggcgtg gctgttgctt ggggaaacag gcgtcatcga gcggaatcgc gcgccagcaa
    23461 gcgggcattt cgagcgcgca atcccaactc cacggccagc aagaaaagca aaaatagggc
    23521 tatccaacgg gaaatatcga tggttccgga ggtggtaccg agacgattct cctggttggt
    23581 ttcggtgatt ctgcgagggc gaaggtccac ttcatgggcg ggaatcgagg cgattcggga
    23641 atcgacttcc tgggcgattc gcaaatcgta gcgccccgct tcgtggggag tgatacgcgt
    23701 tttgccttgg gaatgagcaa ctggataagg gccggatttc gttcggacct ccacggattc
    23761 gctgtcgatt acccaagatt gtccgacatc gattcgcttg gagcccgccc gcaatccaac
    23821 gtcatccacc aattgcttga gcagagcaag aaacgcgggt cgaaagggaa gatcgctcca
    23881 ctgggggctg aaaggaagcc ccacgaccgt tatggtgcca cgtccaagcg ttcgttccac
    23941 gatcagaggc aggttgtcgg accagcgtag gatcgcggtg ggttcggtgg gcccgtcgaa
    24001 tgggaaatgg gctctgcctt tggcttcgag ccgaagcaga cttggaccgc tgggcccgaa
    24061 atgcaggcaa tctcgttcgt tcacgctgtc gggggcatct tcggcccaat gcggtattcc
    24121 ggaaaccaaa ggctcgagcg tggcacccaa aggcgcccgc gccgaccgag gacctagccc
    24181 cacgagggca acaccccctc gctccaccca gccatgcaac gattcccttg tctccggcgt
    24241 gaacccggca ggatcatcga tgaccagaat cgtcagcttg ctcaattcct ccacggtgtc
    24301 aggcatgacc gtcatcggat gaatcgtggc tcccgtatgc aatgcgacaa gcgcttgctc
    24361 gacgggaggt ggacctccag tctcgacgtt ggacagcgaa cgatccgaca ccacgccaac
    24421 ggtgaacgac gtggatcgtc gtgctacggg ggccacgtca ttttccgcaa tgaaatccga
    24481 gcttccctcg agcgcaacct cgtcggggaa agctgatgaa gcgatgggca cgtccaattc
    24541 cgcggttccc gtgtcttgcg gtagatgtgg gaaaataggt tcacgagcga tggtttgtcc
    24601 tgcctcacgc acgaccaaat ttcggtgggg atcacgctgg ttgcccgtac acgccacgcg
    24661 ggcatagacg cgtccacttc gctgctccgc gtataacaca gcgcaatcat tagcattttc
    24721 agaaagctgg cgacttacga tccaggtggg taccgaaagc ccttctccca aaggcggagc
    24781 atcgggcatc ccatcgcaac gatccgtaag gacaaccata cgatgctgtg gttgcggcag
    24841 ttccgaaagc agcgacgcgc ctagtgccat ggcgccttcc agatccgtag ctcggtccga
    24901 aggttcgagt cgttgaagcg tgtgttcggc aagggaaagg tccatggttg gcgcgagaac
    24961 gacacgggcg ggggcgcccg ccaacacgat ggctatggcg tcgccctgtc gcgcgcttgc
    25021 gaataggtcc agggcttgtt gttttgcgag agcgaagcga gtcgtggtgc catcatcgcg
    25081 cgcgcgcatg ctcatcgaat catcgagcac gatggcgaca gccagggaag ctcccccatc
    25141 tcgcgaaatc gacaacttcg agcaagtgag caggggtgta gcgcccaaca cggcaagagc
    25201 gagaattgcc agcagtcgga tactgaataa ggctcgatcc tcgagttttt ttcgttgccg
    25261 agcggaggaa gtcaatgccg gcaccagatg tgcgggcggg aatggctgct ccttcgcacg
    25321 tcgtacgcgc agcaaatgag cggcgaaagg cgcaatgaca agcaatgcga tagccaaagc
    25381 cgcggtgaac acgaaagtca cgggggaacc tcggcaatgg cgtgaagcac tcgaatcacg
    25441 acatccactg gatcttgctg ggtatcgaca cgtaaccaac gtccggctcg attcgcgagc
    25501 ggttgcacga atcgctccgt ccattggtcg agggcttcga gataccgttc gcgaacgaca
    25561 tccggattgg tttcgagcac tgtattgcct tccagagacg cgagacgaac cgtccccgta
    25621 tacggaagtg tcagctcctc atgatcgagg acttggacca cgaccaatcg tcgtccctgg
    25681 ctgccgaggg cggcgaatcg ttcggccgca ccgtcaggca agtcgagaag gtcggatagc
    25741 agaatgacgc aggctccgcg gcgcgctcgt cgggcgatgg actgaatgac gcggtcgaaa
    25801 gcgcgcagat catgcatggc atctcccgtg gcttgcaatc cttctaatgc ttgcacgatg
    25861 cgatcgaagg catcaccgct tgcgctaggc gcgacgggtc gggaaccgac accactgatc
    25921 caaccaagag atacgggatc tcccgcggat aagacgaccc gcgtcagagc cgcgacgatg
    25981 agggcggcga aagccacctt ggctgaatcg gcatgcgcgc cacgaaaact catcgatgcc
    26041 gttgcgtcca gcagcatgtg aacgccgcgg tccgtttccg tttcgaacag gcgaaccaag
    26101 agttgatcgt gacgcatgag ggcatgtcgg tcgatccatc gcaaatcatc cccgggcaca
    26161 tacgcgcgat gaccaccaaa ctccacaccc ggcccatgac gccgactgcg atggacaccc
    26221 gcgaatacgc catcggcaac gcttttggcg cgtagtctca agggcgcaag ccgtccccaa
    26281 tcgatggcct ggttgtggcg ttcactcgga tgcatcacga tgtgcctcgt tgggcggagc
    26341 ttgggcaaaa gccgctcggc taggcgatac ctggcttctg accggaagcg tcaacaagaa
    26401 ggaagagcct tttccgacgg tactctccac ccacacctga ccaccgtggt tggaggcaat
    26461 atgttgcacc aacgccaaac cgatcccact gccacgaacc tgcttgtctc gcgccccttt
    26521 tcctcgaacg aacctatcga agatgcgacg ttgctcatcg atgggcacac ccggcccctg
    26581 atccgtcacg cggatctcaa tggttttcgc ggaacgaaga cgaacctcca cccggatgag
    26641 cttgccgtct ttggcatatt tcatcgcgtt atcgagaaga ttcaccagcg cgagttccaa
    26701 cgcttgctcg tcgagtaccg tgcggggcat gttcgactcg acttgcatgt ccaacaccat
    26761 tccctcccgt tccgctcgat gccggaaggc ttcgaccgcc cgtgaaacca cctccaccac
    26821 gtcggcatat tcgaattgat aggccacttt ccctcgctct aaccgtgcga aatccaacac
    26881 attttctatg agagcggaca atcgttcgct ctcccgcaca atgatggata ggtacttttg
    26941 tcgcttcgct tcgtcgctta ctcgtccggt ggacaacaat tcgctgaaca tcctcaccag
    27001 tgacaatggc gttttcagtt cgtgactcac attggccaca aactcacttt ttagcgccgc
    27061 caaccgtcgc tctcgctccg cagccac
//
LOCUS       DAOSOR010000006        26391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_1845_length_26391_cov_4.443044, whole genome shotgun sequence.
ACCESSION   DAOSOR010000006 DAOSOR010000000
VERSION     DAOSOR010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 26391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26391
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1845_length_26391_cov_4.443044"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..4988
                     /locus_tag="PK710_00705"
     CDS             12..4988
                     /locus_tag="PK710_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08251.1"
                     /translation="MRPEAPGLLDHQDDLVVELARRAQVLENSGTPVALSRALVELSM
                     ALDAYSGDRGEALAQARKACEVAPDVEAAIFVARWFTPARPLGPALELVQTQARLARD
                     DPQRLTRALQTAELYRNASRLEDAQAFYRQALSIQPAHPGALRGCESVLQAQFRVQTG
                     VAPALGGVLEDMASAFTAEPTLSAWLHVERAALLNAAGKHDAARAALELAMAQAFGNA
                     SVRYAYARHLWLQRSTEALVNAWSRQAATEPDASRAARLEYAAGRLASEKLEDANLAI
                     TLHARAAERTQAPVDTRQSALQELARLHAELGRPRGATEAREKLLELVQNPARRCHEH
                     RRLSETYELLGDHDSVVRHCHQLLAVDADDRASRDRLDRALQALNRHHERVEYWTSES
                     ARLAHEKERADALVRAARIAETKLRDVKRAESLYRAAWACDSSNTDAFDGLASLLAHP
                     QSSDNDAHTHRARERIDLYEQAAILATDKERRIACLEKVAIIWEDELRHPTRAMDAYR
                     RIVELDDRRRSALLGLQRCANLAGQVDVLVQTLVKEADIVQDPSLQRTLLLRAAHLTA
                     DEIGDADAAMQWIGRVLARSPGDPLALRAAWYVNRQTGRIEQAVEQLRLLLKHTRKGP
                     AAFSLCMEMAALLEEQLHRPLDAVQAYRDAARHDPEHPAPPIEIPRILLSLGEHRKAA
                     EMLMDLASQMSHPHARSRLLVQAAEIFDDRLDDLDAALMALTQALALVPSDAASFERL
                     VWVQERRGKPTELTPVLEKRIAASSNAEKLMYSMQLAELQQRQRDHAKAAALLQTVVE
                     QDPRQMPALRMLEHSLRKLERWEELAHLLHHEANVTTDPQVKLGALWEALYYETLAAE
                     GAAEKTETALATIEELLVLAPHDLAVHEALLRWTGLPVQGPWGRKLASSLAQLAVFHA
                     ADSFLSGILHLAAAWKLEALGEQEDAAATRQALGHYRACLKQWPHSLTAARGLLRIAQ
                     TVGENEIEVEAHLALAKIEAEAKIRAAHRAAAAEALALQNQPGRSFDLFVDALRDDPD
                     NPNASQGLIALLDRGVDAGHLADTLRKTLDSAHDIDQVVLVGGAMGRIARDVLNDPNG
                     AVEAFRKVRDRAPGHVPSLLELAEACVALQLWFEAAEVAQSVLGLSNQPPHQLQAMLV
                     LAEAHAHTQPKWAEARQKAAEAESRAERLQPAERKIVVARLALVHEALGDNLERDRLI
                     NLSIVLSGSDLSAFHALSTRHDTSTVEGCIAYMQQLNNIVSLSEAMGLPQQPAWLVEM
                     GRLEALRLSRPREGLGKLREAMALEPSRVETVLSLSGTLATLGAHEEAATELRSWLFS
                     VEPATITTEKMAHSMTALHRELVALGRRPQALVADEILAFLGIGPAERTRAYRSRTLA
                     EPIPQPMAFDRAMLENHLIPAAGQGALLQIAAVLDEAAPKLLRLDPSGLASTSAARLS
                     ARSNHPRRALVDRLARAFGAIPCDVYVDVPSLTTPRIIPATPTALLLPPGYDQLPIMD
                     QAVGLTRLLFQAALGVAWLDELNNEDLTGWLFGALAVGRPGWDGGGLHPSKQGPASTW
                     RPTLQKTLSRKARGKLDDIAEEARLDMDPIAWRFAIHLASWRCAYVVTADWTASIHHA
                     WRSNRDLAGVAAERMASSLLAHPVLRDITLWGLSAETTPLLRAVGHGG"
     gene            complement(5312..6559)
                     /locus_tag="PK710_00710"
     CDS             complement(5312..6559)
                     /locus_tag="PK710_00710"
                     /inference="COORDINATES: protein motif:HMM:NF024622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HOT08252.1"
                     /translation="MGYLLTSNLRAGHPDFLDLPWDYPLEQWQRHTERWVDLPAGPSR
                     HPVVFVHYDLNVYALKQLTGSAAEKEYLALRQMQDLRLPVVMPVGHSSTLHDNTEYSV
                     LITRYLDHALPYHSLFMRSSLTRYRDHLLDAMALLLVQLHLAGVFWGDCSLNNTLFRR
                     DAGKLTAYLVDAETAEIHATVSEGMRRHDLDIMEENVQGGLLDLHALGALPQGIDIRA
                     VGPHIRKTYDELWQQVTSEQLIPAGERFRIQERIRRLNDLGFSVHEIELEPSDGGANL
                     RLRAQVTDRYFHRNMLHSLTGLDPEERQARTMLNEIQELKAAMASRQKHSATLSAVAH
                     HWLRETYLPSVERLRPLIDQGSEPAELYCQLLEHKWYLSERASSDVGHATAADDLLCR
                     WRNLPHDVAGVATETDSGSETAG"
     gene            complement(7133..7939)
                     /locus_tag="PK710_00715"
     CDS             complement(7133..7939)
                     /locus_tag="PK710_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibro-slime domain-containing protein"
                     /protein_id="HOT08253.1"
                     /translation="MGRMGTSAYCLSAAALLVVTQSVGCGSNEDNSNANSGNTSSTGG
                     SAGELMEGGPIVPDAMVETTNQLAGVIRDFRDSHPDFESTTGTDKGLVEFELGPDRKP
                     VYAGGDGTLTTHGKKNFDQWFRDVPGVNEGKDFVITLEPSQGNVFTYDNQEFFPIDDQ
                     LFGNEGRKHNYHFTYEINTSFVYMGGEVFRFTGDDDLFVFINGRLAIDLGGVHVAQSK
                     QVDLDDKAEELQIIPGNTYTLDFFFAERHTTESTFRIDTTIGTFLPPPVK"
     gene            8696..9067
                     /locus_tag="PK710_00720"
     CDS             8696..9067
                     /locus_tag="PK710_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08254.1"
                     /translation="MGEKTPPVRPSVPTPFVDHDDTIVMVAPSAMTRVACLDAAIEIG
                     ARPVQVTPENTLKVVATVRPLVIVVEESTQLSSSELTDLAVSVGAQLLVVAPDEVGLT
                     LAERVKLAGRASRILRSRSGE"
     gene            9168..10559
                     /locus_tag="PK710_00725"
     CDS             9168..10559
                     /locus_tag="PK710_00725"
                     /inference="COORDINATES: protein motif:HMM:NF014335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HOT08255.1"
                     /translation="MPPCRSIRILALACVLTVPASPAMAQESEGFHFGSYGRVRAATD
                     LRGGGPQYGNLVAHGSRLDSEPYLELEFREVQYPDDKNKDLRVRVVSTLAFAGDPFHY
                     TGRWESHMALRNMFVDVQGVGDKGLTLWAGARMYRGDDIYLLDFWPLDNLNTVGAGAV
                     LDVGSGTRVQAHVGSNRLLDSDFQYQARLVPSRDDFGTDLAVTLDRPRVLASTKITQY
                     FNGFQAVRGFKSSLVAEGHYLPEGVRETSDNTVRPSHRLPTDTGFVLGAQATGYGFGE
                     RDGHVHLFLRYARGVAAFGDLATPSLLDAKSKAKASEWLFATAGNYEIDRFGIMMGAY
                     ARLFDASLGAEYSANKYWEGIFVTRPQVYLTEHTAVGTEISYQFKNRKVLDDSGRHRV
                     PSAWRLSFLPMITPAGWGGYKRPILYGVYTATFRNDAAKDSYPTEDPRSHRSMEHYLG
                     LHAEWWFNSSSYP"
     gene            10606..12540
                     /locus_tag="PK710_00730"
     CDS             10606..12540
                     /locus_tag="PK710_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOT08256.1"
                     /translation="MKRHNPHLKLSHATPFALALLAVAAGACFDLDDVEGSIQHKTYV
                     QDWRDEVIYQVLVDRFANGDPSNDYGVVPYDPAKYHGGDWKGIEDKLDYIQALGVTTL
                     WISPIVKNVESDAGIDGYHGYWAQDLTKLNPHFGDLAALRSMVDAAHARGLKVILDIV
                     TNHVGQVFFYDINGNGQPDEAVWGGGELIGGRPTTPIQHVTEYDPEYEEPVVMARTSL
                     GEAGPADIIFFRDAATNRMPPVSGYPPNAGYDILQLPQAYNRRGRVWNWGSDHCCYFW
                     LNGKQVPDYCQNDVGTIQCHQVLYGDFPGGLKDVNTEWQEVRDAMFFAYGRWLTLVDF
                     DGFRIDTLKHVEHGFWQDFCPRIRQHAASFGKQQFFMFGESFGGDDRLNGSYTFDNEV
                     DSVFYFSQKYVLDSVFKHGGKTSALKELFDQREANHGTKPHDLGIGLPPTKVLVNFLD
                     NHDVARFLYDKKIGDKGIEALHTALVFLYTEDGIPCLYYGTEQQFEGGNDPNNREDLW
                     NSRYDTSNPTFRFIAELNRIRKRYEPLRRGDLTFVQWNDDSAGILAYERFTSDQRVLI
                     VINTHDSESKETIQTVIDEQTQQPKEQPLPTGFAPGTVLVNVLPDDDPTDELTVAGDS
                     SVRFTVPSRGAKIFVPQNQL"
     gene            12868..15159
                     /locus_tag="PK710_00735"
     CDS             12868..15159
                     /locus_tag="PK710_00735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT08257.1"
                     /translation="MRSWFDIDLDELPDAAFVIGSDGRIARWNLMAEQLFEWSRQDVL
                     GHPLEQFLQFEDGSWRTEAVTQARAGRIFRGTQKVCVRDRTLFLDWSMNGIAPESRDT
                     ATLEIVRDITQRVKREQALNQLFVESPHVVSVIDMTTSQFIQVSRQFCKSQRRDEKEI
                     LGKTAEQIGIVPADNALELKTKLLTDGYLEAEEVQTTKADGSISFGLTSARQLDIDGR
                     KCAVTAWQDITKLKRAEAALRAAHKRFDEAMAVIPVVIWWVTFDQGKATDAYISLEVD
                     RILRLPEGTLRNDWDGLMARIHPEDIAHVQHEIALSFQHPGTNHQVEYRLQRADGTSA
                     WIRTSGVGRLGPNGTIHGFGISEDITAQRLAAAEQERLAQQLQQSQKLESLGRLAGGI
                     AHDFNNILTAILGNLDLVTMQLEPGDPRLARLREAHSAGRKAADLTRHLLALSRQKII
                     EPRVVDLNELVDNASRLLERLLGENIRLRKVLHANLWSVKVDPNQIEQVLINLAVNAR
                     DAMPEGGTLTLETSHVQLDKTYCENHPEATVGPHVMLAVSDTGCGMTPEVKASVFEPF
                     FTTKPPGQGTGLGLAMVHGLIRQHGGSIAFYSEPGQGTTFKVYLPALHVKPTSLGLST
                     HLPTVKGGTETIFLVEDDEGVRRLAGDVLSQLGYRIFSFESVSSALEAASCHNGEIDL
                     LLTDVVLPEHNGRWLADQLLSLFPTMKVLFSSGYTADVIVHHGVLEENIHFLEKPYST
                     DALAIAVRRILDGEELVRLATAL"
     gene            complement(15252..17591)
                     /locus_tag="PK710_00740"
     CDS             complement(15252..17591)
                     /locus_tag="PK710_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOT08258.1"
                     /translation="MNDDTCASISESVDFIRAMIAADNASGKYGGRVVTRFPPEPNGY
                     LHIGHAKSICLNFGIAAENQGVCHLRFDDTNPTTEDTEYVESIQNDVRWLGFDWGPHL
                     YHASDYFQQLYDFAVELIRRGKAYVDSSTEEQIRQRRGTINEPGTPGPYRDRSIEENI
                     NLFDRMRRGEFDDGTHVLRAKIDLAATNMKMRDPLLYRIRKGAHHYRTGDTWCIYPFY
                     DFAHCLSDAIENITHSICTLEFENNRELYDWVLDNVGLMPPRPEQTEFARLNLTYTVM
                     SKRKLLRLVQEKHVKGWDDPRMPTLSGLRRRGVRPSAIRAFCQEIGVARTHNVIDLAR
                     FEHIIRDDLNLDVPRVMCVLRPIEVIIDNYPEDLVETLEAPYYPRDVPRQGTRAVPFS
                     RRLLIERDDFEESPPKGWFRLAPGTEVRLRYAYIIRCERVVKDDAGRVIRLHCSYDPN
                     SRGGQASSDKKVKGTIHWVSENHAVRVQVRLYDRLFVSESPDAEDGDFVRALNPSSLE
                     IVEDACLEPAAAAAIGTEFSHLQFERQGYFSLDPDSQPHALVFNRVVTLRDSWGKRQE
                     KETVPQTPPTNAPSPRSASSSKPTTAETDVEQELSDDQKALRDRIVAKGVPSREARLI
                     ACHPGLAEFFEDACDEASDARAVAKWIVNELLRELKSRVLSSLAFDGKDLGRLVALVT
                     QGTITHTAGKEVFAAMLAGQGKPEAIVGQRGLEQSTSASDLAGIIDRVLHEHAGEVAA
                     YRAGKVALMGFFVGLVMRESQGKANPSSVRSLLQAKLDA"
     gene            17879..19882
                     /locus_tag="PK710_00745"
     CDS             17879..19882
                     /locus_tag="PK710_00745"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOT08259.1"
                     /translation="MTSRILRACSALSLGVAIACVGMTARAEMGFSSDGNLLAMSADD
                     ERGVRVLDLRTGMLSTLTDSPSSGHAFSWSPDGTRLGFKILHPHGDGFLQEPAVYDAT
                     TGVVQSLAAPSSLAGVPSFSAQGHVAFTLGHEAVVIDDNGFVTRHTLPQYVNLVALSP
                     DGKQLVFNASDDSIWLLDRQSDQRRQIAQGPSYGPVWSSDGRRILLSGVDGNVSCVDL
                     ERQTVHRLGRGTSPSWLADGQHIIFTELPVPKGDGQPPQTTVQTVRFDGTERRMLPGV
                     QGYDTRLSPNDTTIVFRSAPQTEPFIARSWNGSTSFSAARPLSEKVKSHRDLRALLRP
                     ARPNVVPPPVTGWRQKPVKIDDVPYLHQVYDTPNHFNGHWACNATSAIMTLAYYGVID
                     FWDTTVNVPSSHVSHYGNYVSEIYTINGKTFDIGSPDPNGNLGYGGYGYIVRNDWKDT
                     KGYMADYIGFHGVDSGVDWSPNWDKVIKEVDNKHPFVVLTSITSAGHYIVDIGYHSDQ
                     HTAIFNDPYGNKNSGYMNYSGANALYDWPGYNNGYSNLVTVHCFIWSRSTVQQPEPEE
                     PDAGTEDVEQPEPDAAEPEASKPDVAIEDSSKPEIGSSSDAGLEASETDARKPTGGSS
                     AFGYSEEPDACACSWVGAHKTQEHAYWSLLACLSLWVLRRRGS"
     gene            complement(19958..21973)
                     /locus_tag="PK710_00750"
     CDS             complement(19958..21973)
                     /locus_tag="PK710_00750"
                     /inference="COORDINATES: protein motif:HMM:NF024930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08260.1"
                     /translation="MPDDTLCVVSRAWVGPTACVLGLLLSSTFSCVGGEGRPDRGSGG
                     HLSGTGGSAAQAGSAGSGAEAGNGTGADAGSGGNSGSGGSGTSGSGGWSGSGGSGTSG
                     SGGTSGSGGSGTSGTGGWAGSGGSSGSGGDAGSGGTGTGGYSGSGGSSGFPGLPPLSP
                     HVTTGGRHACALHDDATVECWGANEFEQAKPPLTQDFHHVSAGDNHTCGIHNDGLLAC
                     WGKSTVFANAPLTRFVQVTSGGTFSCVRAGNGAVSCFGEPPWDLYHPAGTFTHIAAGK
                     DTLCGVKTDGTIECVTLSGENRGSPPAGSYTKVSSGSTAFCALKTSGSAACWSHLPLQ
                     PQPSGPFADITTSAAVACGLNNTGIASCWTHAPSIPPHREDMAPAYALRSIDTSDGFT
                     CGMRPSEEVVCWGTNLAGRTAVNSGPLNHIAAGNTFTCARKISGRPVCWGSNRYGRAL
                     PPGGTFTKIAAGYSHACGLASDGSVSCWGDNQGPVTSVPPGSYSDIASGNGFSCAIRS
                     GQLVCWGNLDKTFSLDAGSYYAVTAGKKHVCALGPGGLVSCFDQYGSVSSPGTLFTSI
                     ASSDGFTCGVTLTNAIHCWGTAAPTPPSITAKQVTCGSAHACAIKSDGHVACWGDNSH
                     GQSSPPNAVFKDISAFSYRTCGIREDGHALCWGYGDASTGENIPPLW"
     gene            complement(22055..23014)
                     /locus_tag="PK710_00755"
     CDS             complement(22055..23014)
                     /locus_tag="PK710_00755"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOT08261.1"
                     /translation="MHRSLTTLALLAFTACAQDAPNQDQPADSSPSLPTRPNEPLGPA
                     GSDHALDILTWNLERFPKRGSETQQHLTTVLSAIQPDIVAVQEIVDASALFEVADAMP
                     GWKADVASFFDPSGPYNPPVGMLWNSTTIHVRQRYLLFDNEDHLAFPRAPLVLEFTWK
                     NIDFVVINVHLKALGDNVIQHDDPNDEEVRRLRACERLEHHIRLHLPHARVIVVGDFN
                     DRIEEPPQTNVFSPFLERPAEYLFADMSIATDPDAVVSYPRYTSHIDHVLITNELFAA
                     TWHPDSFTRTIPVDRVFMTSWGHYASVLSDHRPVLYHLDGSSD"
     gene            23404..25824
                     /locus_tag="PK710_00760"
     CDS             23404..25824
                     /locus_tag="PK710_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08262.1"
                     /translation="MNSGASGKKSGRIWKQGAVGLLALTVLGVAAPACLDRPVAPQQP
                     VTKSLSTQLYQNQKVDKIDLLFMIDNSASMADKQKILAAAVPDLVERLTNPVCVDRES
                     REFKAAVAPGESCPSGTEREFDPILDIHIGVITSSLGGHGADSCSNVPSQQWNPTMED
                     MAHLITRGKDDGGNAVTVDTYEGRGFLNWDPTGEKSNPPGEKNVANLKKNFTYMVRGA
                     DQIGCGYEASLEAWRRFLVDSAPYEKMVPVNCPGGDTANRCRGPEGVDQTVIQQRKDF
                     LRQDSLLAIIMLTDENDCSVIDGGQYYLALQTLDGSAAFHLARGTEACSSDPWSPNCK
                     SCWEVNAADHPECAAGWANPEKDDTLNLRCWEQKRRFGIDFLHPISRYVDALTESRLG
                     DKNKDEKDRELNPVFCTENNLSEDRKTCKASLRDRSLIFLAGIVGVPWQDLANDPDNL
                     KKGYRPVEQLSWTPSNFEANQMPVPNGLPQGKTLWNMILGEVDENGSINPNVDPLDPL
                     MKESLVPRTGTHPITGEDLVQPGSANPVAHTVNGSEWNISKMDDLQYACVFDLGQFGP
                     IDCNLPQNKFGCDCNDSPENPLCWNGSAYGQMQYRAKAYPGRRQLAVLKGMRSQAIVA
                     SVCPANMQNVNEADFGYRPAIGAIVDRLKAALQGTCWDQKLDIETEGDEVGTVPCIVL
                     EATKGDVGADKKAICPPCTGVRSEASKDQIEALKSDPIFKENGLNCVCTINQAKPGPE
                     LEACTRNEVVVGIEGWCYIDPEQDETHNRDIVASCPPANKRLIRFVGDQVPLPGSFTY
                     LQCLAAAL"
     gene            25925..>26391
                     /locus_tag="PK710_00765"
     CDS             25925..>26391
                     /locus_tag="PK710_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT08263.1"
                     /translation="MSRARYPQPTVTKKTVLVVEDEPTLVMGLQDVLEFEGYDVVVAP
                     TGREGVSLANLRKPDVVLLDLMLPDINGYQVCEEIRRRDPFMPIIMLTARSQEADKVR
                     GLDAGADDYVTKPFSIAELTARIRAIVRRASRLPDATPEFIEIGNAKVDVTAQT"
BASE COUNT         5566 a         7566 c         7777 g         5482 t
ORIGIN      
        1 cgccagccag catgcgtccc gaggcgcctg ggttgttgga ccaccaagac gatctggtcg
       61 tggagttggc gcgacgggcg caggttctcg aaaacagcgg aacccccgtg gcgttgtccc
      121 gagcgttggt cgagctttcg atggcgctgg atgcttattc gggggatcga ggcgaggcgt
      181 tggcgcaggc acgcaaagcc tgtgaggtgg cgcccgatgt ggaagcggcg attttcgtgg
      241 cccgatggtt cactcccgca cgtccgttgg gccccgccct cgaattggtg caaacgcaag
      301 cgcggctggc gagggatgac ccccagcggt tgacccgggc cctacaaacc gcggaattat
      361 atcgaaatgc ctcgagattg gaagacgccc aggcgtttta tcgccaggcg ctctcgatcc
      421 aacccgctca tcccggcgcg ttacgagggt gcgaaagcgt gcttcaggcc cagtttcgag
      481 tacaaaccgg tgtagctcca gccctcgggg gagtgttgga ggatatggct tccgccttta
      541 cggccgagcc aaccctttcc gcctggctcc atgtcgaacg ggcagcgctg ctcaatgcgg
      601 caggcaagca tgacgccgcc cgcgcggccc tcgaactcgc catggcccag gcttttggta
      661 acgcatcggt gcgttacgcc tatgcgcgac atctatggct tcaacgttcc acggaagcgt
      721 tggtcaacgc ctggtcccgg caagccgcca ccgagccgga cgccagccga gccgcacgtc
      781 tcgaatacgc cgccggacgc ctggcctcgg aaaaactcga ggacgccaac ctcgccatca
      841 cgctgcatgc ccgtgccgcg gaacgcaccc aagcccccgt ggacacccga caaagcgcgc
      901 tgcaggaatt ggcgagactc cacgcggaac tcggacgccc ccggggcgcc accgaagccc
      961 gcgaaaaact cctcgaattg gtgcaaaacc cggcgcgacg atgccatgaa catcgtcgcc
     1021 tctccgaaac ctacgagctt ttgggcgacc atgacagcgt ggtccgtcat tgccaccaac
     1081 tccttgccgt ggacgccgac gatcgcgcct cgcgcgaccg tctcgaccgc gcgttgcaag
     1141 cgctgaaccg tcaccacgag cgggtggagt attggacatc ggaaagcgcg cggctcgccc
     1201 acgaaaaaga gcgtgccgac gccttggtcc gcgccgcccg tatcgccgaa accaaacttc
     1261 gcgatgtcaa aagggccgaa tccttgtacc gggcggcctg ggcttgtgat tcctcgaata
     1321 ccgacgcttt cgatggcttg gctagccttc tcgcccatcc gcaatcatcg gacaacgatg
     1381 cccacactca tcgcgctcgc gaacgaatcg acctttacga gcaagcagcc atcctcgcca
     1441 ccgacaaaga gcgtcgaatc gcctgtctcg aaaaagtcgc catcatctgg gaagatgaat
     1501 tgcgccatcc cacccgcgcc atggacgcct atcgtcgtat cgtggagttg gacgaccgtc
     1561 gtcgttcggc tctgcttgga ttgcagcgat gcgccaacct cgctgggcag gtcgatgtgc
     1621 tggtgcagac cttggtgaag gaggcggata tcgtacaaga cccctctttg cagcgaacgt
     1681 tgctgcttcg tgcggcccac ttgaccgccg acgaaatcgg cgatgcggat gcggccatgc
     1741 aatggattgg ccgtgtgttg gcgcgcagtc ctggtgatcc cctggcgttg cgtgcagcat
     1801 ggtatgtcaa tcggcagacc gggcgcatcg aacaggccgt cgaacaactc cgtttgctgc
     1861 tcaagcatac acgaaaggga cccgccgcct tctccctttg catggaaatg gccgcattgt
     1921 tggaagaaca gctccatcgt cccctggatg ccgtgcaggc gtatcgtgat gccgcgcgcc
     1981 atgatccgga gcatccggcg ccccctatcg agattccacg gattttgctc agcctcgggg
     2041 agcatcggaa ggcggcggaa atgctgatgg atcttgcctc ccagatgtcc catcctcacg
     2101 ccagaagccg ccttttggtt caagcagcgg agattttcga cgatcggctc gatgatctgg
     2161 atgcggccct gatggccttg acgcaggcgt tggcgctggt tccctcggat gcggctagtt
     2221 tcgaacgact tgtttgggtg caggagcgaa ggggcaagcc caccgaattg actcccgtgc
     2281 tggaaaagag gatcgcagcg tcgtcgaatg ccgaaaaact catgtattcc atgcaattag
     2341 cggagcttca gcagcgacaa cgggaccacg ccaaggccgc ggccctgttg cagaccgtgg
     2401 tcgagcaaga tccgcgtcag atgccagcat tgcgaatgct cgagcattct ttgcggaaac
     2461 tcgagcggtg ggaagaactg gcccatcttc ttcatcatga ggcgaatgtc accaccgacc
     2521 cgcaggtgaa gctgggggcc ttatgggaag cgttgtatta cgaaacgctg gccgcggagg
     2581 gagcggctga aaagacggag acggcgttgg ctacgatcga ggaactcctg gtgctcgcgc
     2641 cccacgatct ggcggttcac gaagcgcttc ttcggtggac cgggctacct gtgcaagggc
     2701 cttgggggcg taaactagcg tcttccctgg ctcaattggc ggtttttcat gccgcggact
     2761 cctttttgtc gggaattttg catctggccg cggcctggaa gctcgaagcc cttggtgaac
     2821 aggaagacgc tgccgccaca cgacaagctc tcggtcatta tcgggcttgt ttgaaacaat
     2881 ggcctcattc gctcaccgcc gcccggggct tgctccgcat cgcgcaaacc gtgggtgaaa
     2941 acgagatcga ggtcgaggcg caccttgcct tggcgaaaat tgaagcagag gcaaaaatcc
     3001 gcgccgccca tcgtgccgcg gcagccgaag ccctcgccct tcaaaaccaa cccggccgta
     3061 gcttcgactt atttgtcgat gccttgcgcg atgacccgga taacccgaac gcgagccagg
     3121 ggctcatcgc cttgttggac cgtggcgtgg atgcgggcca tctggccgat acgctacgaa
     3181 aaaccctcga tagcgctcat gatatcgatc aggtggtgct cgtgggaggc gccatgggaa
     3241 gaatcgcccg cgacgtgctg aacgatccca acggagccgt ggaggcgttt cgaaaagtac
     3301 gggatcgggc gccagggcac gtaccttcgc ttctggaact cgccgaggct tgtgtcgccc
     3361 tgcagctctg gttcgaagcc gcggaagtcg cgcaaagcgt cttgggcttg agcaatcagc
     3421 ctccacacca gctccaggcc atgctcgtgc tggcggaagc ccatgcccat acgcaaccga
     3481 aatgggcaga ggcacggcaa aaagcggcgg aagcggaaag ccgggccgaa cgtctccagc
     3541 ccgcggaacg aaaaatcgtg gtcgctcgcc tggcgctggt ccacgaagcc ctgggagaca
     3601 acctcgagcg ggaccgcctg atcaacctgt ccatcgtcct atccggatcg gatctctccg
     3661 ctttccacgc gctttccacc cgacacgata cctccaccgt cgaagggtgt atcgcctaca
     3721 tgcagcaact caacaacatc gtttccctga gcgaggccat gggtttgccc caacaaccgg
     3781 cctggcttgt ggaaatgggc cgtctcgagg ccctgcgcct gtcgcggcca cgggaaggcc
     3841 ttggaaaact gcgagaagcc atggctctcg aaccatcgcg ggtcgagacc gtactttccc
     3901 tttcgggcac cctcgccacc ctgggcgcgc atgaggaagc cgccacggaa ttgcgttcct
     3961 ggcttttttc ggtcgagcct gccacgataa ccacggaaaa gatggcccat tcgatgacgg
     4021 ccttgcatcg ggagttggta gccctcggtc gtcgccctca ggcgctggtg gctgacgaaa
     4081 tcctggcttt tctgggcatt ggtcccgcgg aacgaacgcg cgcctatcgt tcgcgcacgt
     4141 tggcggaacc tatcccccaa ccgatggcct tcgaccgtgc catgctggaa aaccacctga
     4201 tccctgccgc tggacaaggg gcgttgctgc agatcgcggc cgtgctcgac gaggccgcgc
     4261 ccaaactgct gcgtctcgac ccttccggcc ttgcttcgac gtcggctgca cggttgtcgg
     4321 ctcgctcgaa ccatccccgg cgcgcgctgg tggaccgtct tgcccgggcg tttggagcca
     4381 ttccttgcga cgtatatgtc gatgtaccct cgttgactac accaagaatc atccccgcga
     4441 cgcccacggc tttgctcctt ccccctggat acgaccaact tcctatcatg gatcaagctg
     4501 tgggattgac caggctattg tttcaagcgg ccctgggtgt ggcgtggctg gatgaactca
     4561 acaacgaaga cttgaccgga tggctattcg gagctttggc ggtgggacgg cctggatggg
     4621 atggcggcgg attgcatcct tccaaacaag gtcccgcatc gacctggcgg cccacgttgc
     4681 aaaaaacctt gagtcgaaag gctcggggca aactcgatga tatcgcggaa gaggcccgcc
     4741 tggacatgga tcccatcgcc tggcgtttcg ccattcacct cgcttcttgg cgatgtgcgt
     4801 atgtcgtgac ggcggattgg accgccagca ttcaccatgc atggcgctcg aatcgggatt
     4861 tggcgggggt ggccgcggag cggatggcct cttcgttgtt ggcgcatccg gtgctgcggg
     4921 atatcaccct atgggggctt tccgctgaga caacgccttt gctgcgggcc gtcgggcatg
     4981 gtggataggc ccgaagcgcc tgagccatcc cctcattttc cccaagcatt ttgcttagcc
     5041 cgacgactgc ggccctatgc cacggtccta agccacggga aagcacacaa aacgtccacg
     5101 tccacgtcca cagctacggc cacggccacg gtcacggtca cggctcacgg cccacggccc
     5161 acgcaaaccg gtcacggtca cggtcacggt cacggctcac agctcacgcc acacaaaacg
     5221 gtccaccgtc gcgcgtaaga gcctaccggc tattatataa ttatcaataa attcaaataa
     5281 ttaccacata atcgccagac gattccccaa cctacccggc cgtttcggaa ccggaatccg
     5341 tttcggtcgc aacgcccgcc acatcgtggg gcaggttccg ccatcgacaa agcaaatcgt
     5401 cggcagcggt tgcatgtccc acgtcgctgc tggctcgctc ggacaaatac cacttatgtt
     5461 cgaggagttg gcagtatagc tcggcgggtt cggagccctg atcgatcagg ggacgaaggc
     5521 gttccacgga agggaggtag gtttctcgaa gccaatggtg ggccacggcc gagagcgtcg
     5581 cgctgtgttt ttgcctggag gccatggcgg ctttaagctc ttggatttca ttgagcatgg
     5641 tgcgtgcctg tcgctcttcg gggtcgagtc ccgtgagcga atgcagcata tttcggtgaa
     5701 aataccggtc ggtgacctga gcgcgaagcc gcaggttggc gcccccgtcc gagggttcca
     5761 attcgatttc atggacggaa aagcccaggt cattgagacg gcgtattcgt tcctggatgc
     5821 gaaaacgctc gccggcggga atgagctgtt ccgaggtcac ctgctgccat aattcatcat
     5881 aggtttttcg gatatggggt cctaccgcgc gaatatcgat gccttggggt agcgctccca
     5941 gggcgtgaag gtcgagcagg cccccttgca cgttttcctc catgatatcc aggtcatgcc
     6001 ttcgcatccc ctcggagacg gtggcatgga tctcggccgt ctccgcatct accagatagg
     6061 ccgtgagttt gccggcatcg cgacggaaca aggtattgtt cagggagcaa tctccccaga
     6121 acacgcctgc caggtggagt tgaacgagca gcagcgccat ggcgtcgagc aggtggtcgc
     6181 ggtaacgggt gagactgcta cgcatgaaca gggaatggta gggcagggcg tggtcgagat
     6241 agcgtgtgat gaggacgcta tattcggtat tgtcgtgcag ggtcgaggag tgtccgacgg
     6301 gcatgacgac cggcaagcgc aggtcctgca tctggcgcag ggcgaggtat tctttttccg
     6361 cggcgctgcc cgtgagttgt ttgagggcat agacgttcag gtcgtagtgg acgaaaacga
     6421 cggggtgtcg agaggggccg gcgggcagat ccacccaccg ctccgtatgc cgttgccatt
     6481 gttcgagcgg ataatcccac ggtaaatcaa ggaaatccgg atgtccggca cgcaggtttg
     6541 aggtgagcaa gtaacccatg ggttacacca tagcgttcgc gcggggagcc gacaacgggc
     6601 atccttggat gcgttggttt cccccaatcc gtccatcgct tggtggtcag gagggaatgt
     6661 cattcaggag attgatgggc ttgatcgtag gtcgaaccgt cgaagctcgt ggctcctcca
     6721 acgcgggccg tcccggccgg agcgtaggat gcaacactgg cgcgaagaag ccaatggtgg
     6781 cgtgtggggg tgcctccatg atagtcaggg aagggtaaac tcagcgggtt agtgcaacga
     6841 cacctgatgg gagccgtgat gatttcgatc caatcgttct aggacaaaca gaggcgcaat
     6901 tggctttggc gcatgcatcg tcgtcgtgga acttcgcggc cgcagaagga attggcatag
     6961 acctcgaagg taactatcca acctccgatg aaattgggtt tgctcgcttc ttcgtctggg
     7021 gtttccggaa actcctcgag tgactgggaa aaggcttcgg gctggggtcg gtcctaccaa
     7081 catcaaaagg cttgatgggt gttttcaccc gtctcttgat ggaaagaacg acttacttga
     7141 cgggaggggg caggaacgta ccgatcgtcg tatcgattcg aaacgtggat tcggtggtat
     7201 ggcgctcggc gaagaaaaag tctaacgtat acgtatttcc aggtataatt tgcaattcct
     7261 cggccttgtc gtccaggtcg acttgcttgg attgggcgac atgaacccca ccgagatcga
     7321 tcgccagccg accattgatg aacacgaaaa gatcgtcgtc gccggtaaac cgaaacacct
     7381 ctcctcccat atacacgaag gacgtattga tctcgtaggt aaaatgataa ttatgcttgc
     7441 gcccttcgtt tccgaataac tgatcgtcaa tcgggaaaaa ctcctgatta tcgtaggtga
     7501 agacgttgcc ctgggaaggt tcgagagtga tcacgaagtc tttaccttcg ttcacgccgg
     7561 gaacatcgcg aaaccattga tcaaaattct tttttccgtg ggtggtcaac gttccgtcac
     7621 cgcctgcata taccggtttt cgatcgggcc ctaattcgaa ctcgaccaat cctttgtcgg
     7681 taccggtagt cgattcgaag tcagggtgac tatccctgaa atcgcggatg acaccggcaa
     7741 gctggttggt ggtctcgacc atggcatctg ggacgatcgg gccgccttcc atcagttctc
     7801 cggcggagcc gcctgtagac gacgtattcc cggagttggc gttggaattg tcctcgttgg
     7861 agccacaccc taccgactgc gtcacaacca gaagggctgc cgccgacaga caatacgcgg
     7921 atgtccccat tcggcccatg cgagggtatg tatcacaccc ggagtcctct tgcaagtgtg
     7981 ggccttccgc tcatttctcg ccgcgccttt ccttcaccct acgacgaaca aaaacaagta
     8041 ccgccatcgc taggccatag tcgcggtcac cgctacggct atggcatacg gctatggcat
     8101 acggcacaca aaaatccgca actgcgggcg ggtttgaaac ccgcccctac ggttccggat
     8161 gttgccctga cttcacggcc acggtcacag cccacggcaa acaaaaaacg gtcacggcac
     8221 acacaaaagg gcacaaaacg gccacggcca ccgcgtcttt accatggcag gcccacccga
     8281 gcctttgcgg actaccctct caaccaaaca ccaacgcatc ctggttggac aggaaatcaa
     8341 gctcttcgcg cgcaacgaat acctcgtcga gcgcccggag aaggtcatgg tgccccaagg
     8401 attcgacaaa attctatcaa gtagttgtat ttacagatat ttttataggg gcgggtcaac
     8461 agggggttgc cgtcaacctc aaggtcatgg gaaacaccct gatcgcgtcc tttccaatac
     8521 gagaaatcat gagggctctt tcagaggtac agacaacgaa cccgaaacac ccaacgaacc
     8581 caaccttccc tcggaagccc caaggacttt accctccaag agtctcccca gcgcgtgtcg
     8641 ctctcgcctt gcaaacaggc atatatgccc catgaactat gatacgatgt caacgatggg
     8701 tgaaaaaact ccccccgtac gcccgagcgt accaacgcct ttcgtcgacc atgatgacac
     8761 catcgtgatg gttgccccct cggccatgac gagagttgcc tgtctcgacg ccgccatcga
     8821 aataggcgct cgtccggttc aagtaacccc ggaaaacacg ctcaaggtcg tcgccaccgt
     8881 gcgcccgctg gtcatcgtgg tcgaagagtc cacccaatta tcctccagcg aactcacgga
     8941 cctggccgta agcgttggcg cccagcttct cgtcgtggca cccgacgaag tcggcctcac
     9001 gctggccgaa cgagtgaaac tcgctggccg agcatcgcgg atccttcgca gccgctccgg
     9061 cgaatgagcc gagcctcaca gcgaaatttc aaaaatcgta agccaaatga caaaacggaa
     9121 cgcatctatg cgtgacgttt tcctgggctc tggtaccatg cttacccatg cctccctgcc
     9181 gttcgatacg gatcctcgcc ctcgcttgtg ttctcacggt gccggcctcc cccgcgatgg
     9241 cacaagagtc cgagggattt catttcggct cttatggacg cgtgcgcgcc gccacggacc
     9301 tgcgaggagg tggaccgcaa tacggcaatc tcgtggccca tggcagtcgt ctcgattccg
     9361 aaccctacct cgaactggag tttcgcgaag tccagtaccc cgatgacaag aacaaggatt
     9421 tgcgggtgcg ggtggtaagc accctggctt tcgccgggga ccccttccat tatacgggtc
     9481 gctgggaatc ccatatggcc ttgcgcaata tgttcgtgga tgtgcagggc gtgggcgaca
     9541 agggcctgac cttgtgggct ggcgcgcgca tgtatcgcgg cgatgacatc tatcttctcg
     9601 acttctggcc gctggacaat ctcaatacgg tcggtgctgg ggccgtactc gatgtcggtt
     9661 cgggaacgcg ggtgcaggcc catgttggtt ccaatcgatt gttggattcg gattttcaat
     9721 accaggcccg gttggttcct tcgcgtgacg acttcggaac ggatttggcg gtcaccctcg
     9781 atcgtccgcg cgttctcgct tccacgaaaa tcactcaata tttcaatgga ttccaagctg
     9841 tccgggggtt caaatcctcg ctggtcgccg aagggcatta cttgcccgaa ggcgtacgcg
     9901 agacctcgga caatacggtg aggccttccc atcgattgcc cacggacact ggattcgtgc
     9961 tgggagcgca ggccaccggg tatggttttg gcgaacggga tgggcacgtt catttattct
    10021 tgcgttatgc gcgcggcgtg gcggccttcg gcgaccttgc gaccccttcg cttctcgatg
    10081 ccaaaagcaa agcgaaagca tccgaatggt tgttcgccac cgccggcaat tatgaaatcg
    10141 accgtttcgg gatcatgatg ggcgcgtacg cacgcctttt cgatgcctcc ctgggcgcgg
    10201 agtattccgc caacaagtac tgggaaggca tcttcgtcac acggccccag gtctatctca
    10261 ccgagcatac ggctgtggga acggagatca gctatcaatt caagaatcga aaagtacttg
    10321 acgattccgg gcggcaccgt gttccttccg cgtggaggct gtcttttcta cccatgatca
    10381 cacccgcggg gtggggaggt tataagcgcc ccatattata cggagtgtat accgccacct
    10441 tccgcaacga tgccgcaaag gacagttatc ccacggaaga tcctcgctcc catcgaagca
    10501 tggaacacta cctcggcttg catgccgaat ggtggttcaa ctccagttct tacccgtgac
    10561 ccaacccgga tcgtcgatgc catggctcat gcccgaggaa aaaccatgaa gcgccacaac
    10621 ccgcacttga agctatccca cgccactccc ttcgccctcg cgctgcttgc cgtcgccgct
    10681 ggagcctgtt tcgatctcga tgatgtggaa ggctcgattc agcacaaaac ctacgtgcaa
    10741 gactggcgcg acgaagtcat ctaccaggtg ttggtggacc gattcgccaa tggcgatccc
    10801 tccaatgatt atggtgtcgt gccctacgat cctgccaagt atcacggagg cgattggaaa
    10861 gggatcgaag acaaactcga ttatattcaa gcccttgggg tcacgacgtt atggatctct
    10921 cccatcgtca agaacgtgga aagcgacgcg ggtatcgacg ggtatcatgg gtattgggcg
    10981 caggatttga cgaagttgaa tcctcatttt ggggatctgg cggctcttcg ctcgatggtg
    11041 gatgcggccc atgcccgggg gctgaaggtc attctcgaca tcgtcaccaa ccatgtgggt
    11101 caagtatttt tctacgatat caacggcaac gggcagcctg acgaagccgt atggggagga
    11161 ggagaactca tcggaggccg accgaccaca cccatccaac acgtgacgga atacgatccc
    11221 gaatacgaag aaccggtggt catggctcga acctcccttg gagaagccgg acctgcggat
    11281 atcatttttt tccgcgatgc ggccaccaac cgcatgccgc ccgtctccgg ttatccgccc
    11341 aacgcgggct atgacatcct gcaattgcca caagcttaca accgtcgcgg ccgcgtttgg
    11401 aattggggaa gcgaccattg ctgctacttc tggctcaacg gcaaacaggt gcctgactat
    11461 tgccaaaacg acgtgggaac catccaatgc catcaggtcc tgtacggcga ctttcccgga
    11521 ggtctgaagg acgtcaacac cgaatggcag gaagttcgag acgcgatgtt tttcgcctat
    11581 ggtcgctggc tcacactcgt ggacttcgac ggatttcgca tcgacaccct caaacatgtc
    11641 gagcacggat tctggcaaga cttctgccca cgcattcgcc agcacgccgc aagcttcgga
    11701 aaacagcagt ttttcatgtt tggggaaagc tttggcggcg acgatcgtct caacggcagc
    11761 tataccttcg acaacgaagt cgatagcgtc ttttatttct ctcagaagta cgtgctcgac
    11821 tccgtattca aacacggcgg caaaacgagt gccctcaaag aactttttga tcagcgcgaa
    11881 gccaaccacg gaaccaagcc tcatgatttg gggatcggac tgccgcccac caaagtgctc
    11941 gtcaacttcc tggacaacca cgatgtagcc cgcttccttt acgacaaaaa aatcggcgac
    12001 aaaggtatcg aagccctgca taccgccctc gtttttttgt acacggaaga cggcattcct
    12061 tgcctatatt acggcacgga acagcaattc gagggaggca acgatcccaa caaccgcgag
    12121 gatctctgga actcccgata cgatacctcg aacccaacgt tccgattcat cgcggaactc
    12181 aaccgcattc gcaaacgcta cgagcccctg cgtcgcggcg atctcacctt cgtgcaatgg
    12241 aacgacgact ccgcgggcat ccttgcctac gaacgcttca cctccgacca gcgcgtgttg
    12301 atcgtcatca acacccatga ctcagaaagc aaagagacca tccagactgt gatcgatgag
    12361 caaacgcaac agcccaaaga gcaaccactt cccacgggct ttgcgccagg cacggtcctt
    12421 gtcaacgtct tgcccgacga cgatcccacc gatgagctga cggtcgccgg tgatagcagc
    12481 gtgcgcttca ccgtaccttc gcgtggggcc aagattttcg tgccccaaaa ccagctatga
    12541 gttggagcgg aagccgtcga ttccctcccc tcccctacgg ctgcccccaa tcgccacgac
    12601 tgccataagc aattgaaatt acatagtttt tatttctaag aggcgatgtc gcacaattgc
    12661 ctttcccttg cgtgggcatg cccctgggga accgatcggg cgagcgctcg atcgacggca
    12721 tgagcaacac gacatgcatt cttgtatcgg gcaacgtggt gagcaacgtt tcgggcaggg
    12781 cgaaatgaat aatcgttcgg tgatacccct agccgcttca tcctccttgc tttatgatcc
    12841 ggttctctca ctcgctcggg ggagacaatg cgatcatggt tcgacatcga tctcgatgaa
    12901 ctgcctgacg cggctttcgt cattggttcc gatgggcgca tcgcccggtg gaatctcatg
    12961 gccgagcagc ttttcgagtg gtctcgacag gatgtcctcg gccatcctct cgagcaattt
    13021 ctgcagttcg aagatggctc gtggcgaacc gaagcggtga cgcaagcgcg agcgggtcgc
    13081 atctttcgcg gtacgcagaa ggtctgcgtg cgggaccgaa cgcttttcct cgactggtcg
    13141 atgaatggca tcgccccgga gtcacgagat acggccacgc tagaaatcgt gcgtgacatc
    13201 acccagcgag tgaaaagaga gcaagcgctc aatcagcttt tcgtggaaag cccgcatgtc
    13261 gtttcggtca tcgacatgac gaccagccaa ttcatccagg tgagtcgtca attctgcaag
    13321 tcccaaaggc gtgacgaaaa agagattctc ggcaaaaccg cggaacaaat cggaatcgtt
    13381 cccgcggata acgctttgga attgaagacc aagttgctga cggatgggta tctcgaagcg
    13441 gaagaagttc agacgacgaa agcggatggt tcgatttcgt ttgggctgac gtcggcgcgc
    13501 caactcgata tcgacgggcg aaaatgtgcg gtcacggcct ggcaggacat cacgaaattg
    13561 aagcgagcgg aggctgcctt acgggcggcc cataagcgtt tcgatgaggc catggcggtg
    13621 attccggtgg tgatttggtg ggttaccttc gaccagggca aggccacgga cgcctatatt
    13681 tcgctggaag tcgaccggat tttacgtctg cccgagggaa cgttgcgcaa cgattgggac
    13741 ggattgatgg cgcgaatcca tccggaagat atcgcccatg tgcaacatga aatcgcgttg
    13801 agttttcaac acccaggcac caatcatcaa gtcgagtatc gtctgcagcg cgccgacgga
    13861 acctcggcct ggatccgaac ctcaggggtg ggaagactcg gcccgaacgg aaccatccat
    13921 ggattcggaa tctccgaaga catcacggcc caacgtcttg ccgcggctga acaagagcgg
    13981 ctggcccagc agcttcaaca atcccaaaag ctcgaatcgc tgggaaggct cgctggagga
    14041 atcgctcatg atttcaataa catcctgacc gcaatcctcg gcaacctcga ccttgtgacg
    14101 atgcagttgg aacctggaga cccgcgtctt gcaagattgc gcgaagccca ttccgctggg
    14161 cgcaaagccg cggacttgac acgtcacctg ctcgccctat cccgtcaaaa aatcatcgaa
    14221 ccgcgtgtcg tggacctcaa cgaattggtc gacaatgctt cccgtctgct cgaacgtctt
    14281 ctcggcgaaa acattcggct tcggaaggtg ttgcatgcga acctttggtc ggtgaaggtc
    14341 gatccgaacc agatcgaaca agttctcatc aatctcgcgg tcaacgcgcg cgacgccatg
    14401 ccggagggcg gaaccctcac gctcgagacg tcccacgtgc agctcgacaa gacctattgt
    14461 gagaatcatc ctgaagccac cgtgggcccc cacgtgatgt tggcggtcag tgacacgggt
    14521 tgcggcatga cccctgaggt gaaagcatcg gttttcgagc cgtttttcac gaccaaaccg
    14581 ccaggccaag gcacggggct cgggctcgcc atggtgcatg gtttgattcg ccagcatggg
    14641 ggctcgatcg ctttttattc cgaacccgga cagggaacga ccttcaaagt ctacttgcct
    14701 gcccttcacg tcaagccgac ttccttgggg ctctccaccc accttcccac cgtgaaaggc
    14761 ggaacagaaa ccatctttct ggtggaagat gatgagggcg tacgacgact tgcgggcgat
    14821 gtgctttcgc agctcggcta ccgaattttt tctttcgaat ccgtgtcatc ggctctcgaa
    14881 gcggcttcgt gccacaacgg tgagatcgac ctattgctca cggacgtggt gttgcccgaa
    14941 cacaacgggc gatggctcgc ggaccaacta ttatcccttt tccccacgat gaaagtgctg
    15001 ttttcctcgg ggtataccgc tgatgtcatc gtgcatcacg gcgtgctcga agagaacatc
    15061 cactttttgg aaaaacccta ttcgaccgac gcgctcgcca tcgccgttcg cagaatcctc
    15121 gacggtgagg agttggttcg tctcgcgacc gctttataga aactctaata aatataaata
    15181 gttatcgaat gaacgggcag gctcggtttc gaagtcgaaa cggaaggctt gccctttgcc
    15241 atcgtcgatg ctcacgcatc gagtttcgct tgcaggaggc tacgaacgga tgacgggttg
    15301 gctttgccct ggctttctcg catgaccagg ccgacgaaaa agcccatcag cgccactttt
    15361 ccggctcggt aagcggcgac ttccccagca tgttcgtgca acacccgatc gatgataccg
    15421 gcaaggtcgc ttgccgatgt gctttgttcg agcccccgct ggccgacgat cgcttctggt
    15481 ttgccttgtc ccgcgagcat cgccgcaaac acctcttttc ctgccgtatg cgtgatcgtg
    15541 ccttgggtca cgagtgcaac gaggcgtcca aggtctttgc catcgaaagc aaggcttgaa
    15601 agcacgcggc ttttgagttc gcgaagaagc tcattgacga tccacttggc aaccgcccgt
    15661 gcgtcgcttg cttcgtcgca agcatcctcg aaaaactctg ccaaccccgg gtgacaagcg
    15721 atgagtcgag cctctcggga gggaacgcct ttggccacga ttcgatcgcg aagggctttt
    15781 tgatcgtcgg aaagctcctg ctcgacatcc gtttctgccg tcgttggttt cgacgaggag
    15841 gcggagcgag gggagggggc attcgtcggg ggagtctgcg gaaccgtttc tttttcctgt
    15901 cgttttcccc agctatcgcg aagggttacg acacggttga agacgagggc gtgaggttgg
    15961 ctatccgggt cgagcgagaa gtaaccttgt cgttcgaatt ggagatgaga aaactcggtg
    16021 ccaatcgccg cggcagcggc gggttcgaga cacgcatcct cgacgatttc cagcgatgaa
    16081 gggttcaagg cgcgcacgaa atcaccatct tcggcgtccg gtgattcaga tacgaaaagg
    16141 cgatcgtaca gtcgtacttg cacccgaacc gcgtggtttt ccgaaaccca atggatggtg
    16201 cctttgactt ttttgtcgct cgacgcctgc cctccccggc tgttgggatc gtaggagcaa
    16261 tgaagtcgaa tgactcgccc cgcatcgtct ttgacgactc gttcacaccg aatgatgtag
    16321 gcgtaacgaa ggcgtacttc ggtgccggga gcgagacgga accacccttt gggcggactt
    16381 tcctcgaaat catctcgctc gatcagcaag cgacgggaga acgggacggc gcgcgttccc
    16441 tgtcgcggca catcacgcgg ataatagggc gcttccaacg tctcgacgag atcttcgggg
    16501 tagttgtcga tgatcacctc gatgggacga agcacgcaca tcacgcgggg gacatcgagg
    16561 ttgaggtcat cgcggatgat atgttcgaat cgggccaaat cgatgacgtt atgggttcgc
    16621 gccacaccga tctcttggca aaaggcgcga atggccgagg gacgaacacc acgccgtcgc
    16681 agccccgaaa gcgtgggcat acgcgggtcg tcccaccctt tgacgtgctt ttcttgcacg
    16741 agtcgcagca gcttgcgttt gctcatgacg gtatacgtca agttcaggcg tgcgaattcc
    16801 gtctgctcgg ggcgcggtgg catcaatccc acgttgtcca acacccaatc gtataattct
    16861 ctgttgttct cgaattcgag tgtgcaaatc gaatgcgtga tgttttcgat ggcatccgaa
    16921 aggcaatggg cgaaatcata aaacgggtag atgcaccaag tatcccccgt gcgataatga
    16981 tgtgcccctt ttcggatacg gtacaacagc gggtcacgca tcttcatatt cgtggcggcc
    17041 aggtcgattt tggcgcgaag gacatgcgtg ccgtcatcga actcaccacg tcgcatccta
    17101 tcgaataaat tgatattttc ctcgatcgac cgatctcgat acggtccggg ggtgcccggt
    17161 tcattgatgg ttccccgtcg ttgtcggatc tgctcttcgg tcgaactatc cacataggct
    17221 ttgccgcgtc gaatgagttc gaccgcgaag tcgtagagtt gttggaagta gtcggaggca
    17281 tgatatagat ggggccccca gtcgaagccg agccagcgaa cgtcgttttg aatggactcg
    17341 acgtattccg tatcttccgt ggtgggattg gtgtcgtcga agcgcaggtg gcagacgccc
    17401 tgattttcgg cggcgatacc gaagttcagg cagatggact tggcatgtcc gatatgcaag
    17461 tatccgttgg gttccggagg aaagcgcgtc accacgcgtc cgccgtactt tcctgacgcg
    17521 ttgtccgcgg cgatcatggc gcggatgaag tcaacggact cgctaatcga agcgcaggtg
    17581 tcatcgttca cggcagaact cctttgctgc cttggcagca ttcatgcacg cgcggaatac
    17641 cccgagcgtc gagcttcggc aactgtcgag accatgggcc attgcctgaa ggcgggccat
    17701 ggctccccta cgccttgctt tcgccccgaa gatcgttgcg gatctgccgg atctcactgg
    17761 gatattgcat ctcccgggcg gtggattttg ccttatcatt tcggctcatg cgtggtctcc
    17821 tcctgtcttg gatgaatggc acgacagttg cttgtacgtg tccaaaggtg gtatgccgat
    17881 gacttctcga atccttcgtg cttgctctgc actatccctg ggtgttgcca tcgcttgcgt
    17941 gggtatgacc gcgcgcgcgg agatgggatt ctcctccgac ggcaacttgc tcgccatgtc
    18001 cgctgacgac gagcgtgggg tgagagtgct cgatcttcgt acgggtatgc tgtcgacgct
    18061 gaccgattca ccctcgagcg gccatgcttt ttcctggtcg cccgatggaa cccgattggg
    18121 tttcaagata ttgcatcctc acggggatgg tttcctgcaa gaaccagccg tatacgatgc
    18181 aaccacgggc gtcgttcaat cgctggctgc tccttcatcc ctcgcgggcg ttccctcttt
    18241 ttccgcccaa ggacacgttg ctttcacgct cggccacgag gcggtggtga tcgacgataa
    18301 cggattcgtc acgcgtcata cgcttccgca atacgtcaac ctggtagcct tatctcccga
    18361 tgggaaacag cttgttttca acgcttccga cgattcgatc tggttgttgg atcggcaaag
    18421 cgaccaacga cggcagatcg cacaaggtcc atcgtatggt cctgtgtggt cttcggatgg
    18481 ccggcgtatt ttgctcagcg gcgtggatgg gaacgtatct tgtgtggacc tcgagcggca
    18541 aacggttcat cggcttgggc gcggcacgtc accatcgtgg cttgccgatg ggcaacacat
    18601 catttttacg gagttgccag tgcccaaagg cgatggtcaa cctccccaga ccacggtcca
    18661 gaccgtacgt ttcgatggaa cggagcgtcg gatgttgccc ggcgtgcaag gttatgacac
    18721 tcgcctttcc cccaacgaca ccacgattgt atttcgttcg gcaccgcaaa ccgagccgtt
    18781 catcgcccgg tcatggaatg gatcgacttc gttttccgcg gctcgacccc tgagcgagaa
    18841 ggtaaaatcg catcgagatc ttcgagcgtt gcttcgccct gctcgtccga acgtcgtgcc
    18901 gccgcccgtc accggatggc gtcaaaaacc cgtaaaaatc gacgatgtcc cctatttgca
    18961 ccaggtgtat gacacgccca accacttcaa cggccattgg gcttgcaatg ccacttccgc
    19021 catcatgacc ctcgcctatt acggtgtgat cgatttctgg gacacgaccg tcaatgtacc
    19081 ttcgtctcac gtgagccatt acggcaatta cgtttccgag atttatacca tcaacgggaa
    19141 aactttcgac atcggctcac cggaccccaa cggtaatctt ggctacggtg gatatgggta
    19201 tatcgttaga aacgattgga aagacaccaa gggctacatg gcggactaca tcgggtttca
    19261 cggtgtcgat tccggcgtgg attggtctcc caactgggac aaggtcatca aggaggtgga
    19321 taacaagcat ccgttcgtcg tgctgacctc gatcacgtcc gcgggccatt atatcgtgga
    19381 tatcggctac catagcgatc agcatacggc gatcttcaac gatccgtacg gcaacaaaaa
    19441 cagcggctac atgaattata gcggcgccaa tgccctgtac gattggcctg gctacaacaa
    19501 cggttactcc aatctcgtca ctgttcattg cttcatctgg agccgctcga ccgttcaaca
    19561 acccgagccc gaagagccgg acgcgggcac cgaggacgtc gaacagcctg agccggatgc
    19621 ggctgaaccg gaagcctcga aacccgatgt ggctatcgag gattcgtcga aaccagaaat
    19681 cggatcgtcg tcggatgcgg ggctggaagc gagcgaaacc gacgcccgca aaccaacggg
    19741 aggttcgtcg gcgttcgggt atagcgaaga gccggatgct tgcgcttgtt cttgggttgg
    19801 tgcccataaa acccaagagc atgcttattg gagtctgctc gcttgtttgt cgttgtgggt
    19861 ccttcgacga cggggctcat gaacgacggt tgaatgcatt gttggatgca ttgtggatgt
    19921 attttcggcg tattctcggt caattgttgg ggaaggctta ccagagcggc gggatatttt
    19981 ctcccgtgga ggcatcgccg tagccccagc acagcgcgtg gccgtcttct cggatcccgc
    20041 aagtgcggta agaaaaggcg gaaatatcct tgaaaaccgc gttgggggga gagctttgcc
    20101 catggctatt gtcgccccag caggccacgt ggccgtcgga tttgatggcg caggcatggg
    20161 cgcttccgca ggtgacttgt ttggcggtga tggagggggg cgtgggtgct gctgtccccc
    20221 agcagtgaat ggcgttggtg agggtcacac cgcaggtgaa gccatcgctg gaggcgatgc
    20281 tcgtaaagag cgtgccaggc gatgataccg aaccatattg gtcgaagcag gagacgagac
    20341 ctcccgggcc gagggcgcaa acatgctttt tccctgcggt tacggcgtaa taggagcctg
    20401 catcgagcga gaaggttttg tcgagattgc cccagcacac caattgtccc gatcgaatgg
    20461 cacaagaaaa gccatttccg gaggcgatat cgctatagga gccgggcggc acggaggtca
    20521 cgggcccctg attatctccc cagcacgaca cggagccatc gctcgccaat ccgcaagcat
    20581 gcgaatagcc ggccgcgatt ttggtgaagg ttccgccggg gggcaaggcc cgaccatagc
    20641 ggttgctgcc ccaacaaacg ggtcgtccac ttattttgcg ggcgcaagtg aaggtattgc
    20701 ctgcggcgat atgattcagg gggccggaat tgaccgcggt ccggccggcc aggttcgttc
    20761 cccaacacac cacttcctcg ctggggcgca tgccacaggt gaagccgtcg gacgtatcga
    20821 tgctacggag agcataagcg ggggccatgt cttctcggtg cgggggaata gaaggggcat
    20881 gggtccagca ggaggcgatg ccggtgttgt tgaggccgca agccacggca gcggaagtgg
    20941 tgatatcggc aaagggtccc gagggttgag gttgcagggg aagatgcgac cagcatgcgg
    21001 ccgatccact ggttttcaag gcgcagaaag cggtggagcc cgaggacact ttggtgtaag
    21061 agcctgccgg gggggagcct cggttttcgc cggaaagcgt aacgcattcg atagttccgt
    21121 ctgtcttgac tccgcagagg gtatcctttc cagcagcgat atgggtgaaa gttccggcag
    21181 gatgatacag gtcccaggga ggttcgccga agcaggaaac cgcgccgttg ccggcacgaa
    21241 cgcaactgaa agtacctccc gaggtgacct ggacgaagcg ggtcaacgga gcgttggcga
    21301 agaccgtcga tttgccccag caagccaata ggccgtcgtt gtgaatgccg caggtatggt
    21361 tatcgcccgc ggaaacatgg tggaagtctt gggtgagggg gggcttggct tgttcgaatt
    21421 cattggcgcc ccagcattcg acagtggcgt cgtcgtgcaa ggcgcaggcg tggcgtccgc
    21481 cggtggtgac gtggggagaa aggggcggga ggccagggaa accggatgag ccaccggagc
    21541 cgctatagcc gccggtgcct gttccgccgg agcctgcatc accgcccgat ccgctcgaac
    21601 caccggagcc tgcccatccg ccggtgccac tggtgccgga gcctcctgag ccgctggtgc
    21661 cgcccgagcc gctggtgccc gaaccaccgg agccgctcca tccgcccgag ccgctggtac
    21721 cggagccccc ggagccgctg ttgccgccgg agccagcatc ggcgcccgta ccgttgccag
    21781 cttcggcacc tgatcctgcg cttccagcct gggcggccga gcccccggtg ccggaaaggt
    21841 ggcctccgct acctctatcg ggccgccctt cgccccctac gcaactgaat gtactcgaaa
    21901 gcagcagtcc gaggacgcag gccgtggggc ccacccaagc ccgggaaacg acacaaaggg
    21961 tatcatccgg catctattgg gcttaacgcg gggccgccca ccggtcaagg atgcccgtgc
    22021 agggggcaag cgcttcttat cgatacgacc tccgtcaatc ggaggagccg tccaggtggt
    22081 aaagcacggg tcgatggtcc gacaggacgg aggcataatg cccccagctc gtcatgaata
    22141 ctcggtccac gggaatggtt cgggtgaagg agtcagggtg ccaggtggca gcgaatagct
    22201 cgttggtgat gaggacatgg tcgatatggc tggtgtaacg gggataggag acgacggcgt
    22261 ccgggtcggt agcgatggac atatcggcga aaaggtattc ggcgggtcgt tccaggaacg
    22321 gggaaaagac attggtttgc gggggttctt cgattctgtc gttgaaatct ccgaccacga
    22381 tgacgcgggc gtgggggagg tggaggcgga tatgatgttc gagacgttcg caagcgcgga
    22441 gacgtcggac ttcttcatcg tttgggtcat catgctggat gacgttgtct cccagggctt
    22501 tgaggtggac gttgatcacc acgaagtcta tgtttttcca ggtgaattcc aataccaggg
    22561 gagcgcgtgg gaaggcgaga tggtcttcgt tatcgaagag cagatagcgc tgtcggacgt
    22621 ggatggtggt tgaattccag agcataccga cgggtgggtt ataggggccg gaggggtcga
    22681 agaaggaggc gacgtcggct ttccaaccgg gcatggcgtc ggccacctcg aaaagggcgg
    22741 aggcgtcgac gatttcctgg acggcgacga tatcgggttg gatggcggac aggacggtag
    22801 tgaggtgttg ttgggtttcg gagcctcgtt ttgggaagcg ttcgaggttc caggtcaaga
    22861 tatcgagggc atggtcggag cctgcggggc cgaggggttc gttagggcgg gtgggcaagg
    22921 agggggagga gtctgcgggc tgatcttggt tgggggcgtc ttgggcgcag gcggtgaatg
    22981 cgagcaaggc gagggtagtg agggaacgat gcacgactgg ctccgtgcga gaagcgtagg
    23041 gcatatgggg ctgggagcca agcgaagtgg ttgcccatcg caatgggttg tggggccaac
    23101 aagcaactca gttggcagtt gcaagaaaaa ttgcctattc agtaggcaga atgaaggaag
    23161 ggtaaggagg ggggcaattg ggtgggatca ttggggaaaa gggcatcaac gggccaaggg
    23221 gcaacttggc ttggatcgtg ctgtgcctca gggatggcgc ttggattatc ggtaggctac
    23281 cctaacagct tggattgaca tggatgtggt cagccacgta aggtgccagc ccatttggta
    23341 tcgaaggtcg cccgtgcatg gttcatgcta gagcgggatt gacgatggaa ggagaagttg
    23401 cggatgaaca gtggagccag cggcaagaaa agcggacgca tctggaagca gggtgcggtg
    23461 ggtctattgg ccttgaccgt actcggggta gctgccccag cgtgccttga caggccggtg
    23521 gcgccacagc agcccgtgac caagagtttg tcgacgcagc tttaccagaa ccagaaggtc
    23581 gacaagatcg acctattgtt catgatcgac aactccgcat cgatggcgga caagcaaaaa
    23641 attcttgcgg ccgcggtccc ggaccttgtc gaacggctta ccaatcccgt ttgtgtcgac
    23701 cgggaatcgc gagaattcaa agcggcggtt gcgccagggg agagttgtcc gagcggaacc
    23761 gagcgtgaat tcgaccccat cctcgatatc catattggcg tcattacttc ttctctcggc
    23821 ggtcacggtg cggattcctg cagcaacgta ccttcgcagc aatggaaccc caccatggag
    23881 gatatggccc atctgatcac gcgtggaaaa gacgatggcg gcaatgccgt taccgtcgac
    23941 acctatgagg ggcgtggttt tttgaattgg gatccgactg gggaaaagag caatccaccg
    24001 ggtgagaaga acgttgccaa tctgaaaaag aactttacgt acatggttcg aggtgcggac
    24061 cagattggtt gtggttacga agccagcctc gaggcgtggc gacgattcct ggtggactcc
    24121 gctccttatg agaagatggt gcctgtcaac tgcccggggg gagatactgc caaccgctgt
    24181 cgcggccccg agggtgtcga ccaaacggtg attcaacaac gcaaagattt ccttcgccag
    24241 gattcattgc tcgcgatcat catgctcacg gatgaaaacg actgttcggt catcgatggt
    24301 gggcagtatt atttggcgtt gcagacgctc gacggcagtg cggctttcca tctggcccga
    24361 ggcacggaag cttgttcgtc ggacccatgg agtccgaatt gcaagtcttg ttgggaagtc
    24421 aatgccgcgg accatccgga atgtgcggcg ggctgggcca atcccgaaaa agacgacacg
    24481 ctcaaccttc gctgctggga gcagaaacgt cgcttcggca tcgatttcct tcatccaatc
    24541 agtcggtatg tcgatgcgtt gacggagtcg aggctcggcg acaaaaacaa agacgaaaaa
    24601 gacagagaac ttaacccggt gttttgcacg gaaaacaatc tgagcgaaga ccgaaagacc
    24661 tgcaaagcat ccctgcgtga ccgttccctc atctttttgg ctggtatcgt cggggttccg
    24721 tggcaggacc tcgcaaacga tccagataac ttaaaaaagg gatatcgtcc tgtcgagcag
    24781 ctttcctgga caccgtccaa cttcgaagcc aaccagatgc ccgtacccaa tggcttgccc
    24841 caaggaaaaa ccttgtggaa catgattctt ggggaggttg acgaaaacgg atccatcaat
    24901 ccgaacgtcg acccgttgga tcctttgatg aaggaatctt tggtgcctag aacgggcacg
    24961 catccgatca ccggagagga cttggttcaa cccggaagcg caaaccccgt ggctcatacc
    25021 gtcaacggtt ccgagtggaa catctcgaag atggacgact tgcaatacgc ctgcgttttc
    25081 gacctcggac agtttgggcc catcgattgc aatctccccc aaaacaagtt cggatgcgac
    25141 tgcaacgact cgcctgaaaa cccgctttgc tggaatggaa gcgcttacgg gcagatgcaa
    25201 taccgcgcca aagcctatcc gggtcgtcgt cagctcgcgg tgctcaaagg catgcgttct
    25261 caagccattg tcgcttcggt ttgccctgcc aacatgcaaa atgtcaacga agcggacttc
    25321 ggttatcgcc ctgcgattgg cgcgatcgtc gatcgtttga aggcggctct gcaaggcacc
    25381 tgctgggatc aaaaactcga catcgaaacc gaaggggatg aagtcggtac cgtgccttgc
    25441 atcgtgctcg aagctaccaa aggcgatgtg ggggcagaca aaaaagccat ctgtcctcct
    25501 tgcaccggag ttcgctctga agcgagcaaa gaccagatcg aggctctcaa gagtgacccc
    25561 atcttcaagg agaacggttt gaactgcgtc tgcaccatca accaggccaa accaggaccg
    25621 gaactcgaag cttgcacacg aaacgaggtg gtggttggta tcgagggctg gtgttatatc
    25681 gacccggagc aggacgagac ccacaaccgt gatatcgtgg cttcttgccc accggcgaac
    25741 aaacgcctca tccgtttcgt aggcgaccaa gttccgctgc ctggctcctt cacctacttg
    25801 caatgcctgg ccgcagccct ctaggtttcg tcggcacttc cgacgacagc agccagggcg
    25861 attgaatcct tccgctgaaa gttttccctc ttccgccaca agattctgac taaggcttgt
    25921 cgccttgtct cgagcgcggt atccacaacc caccgtgacc aagaagaccg tcctcgtcgt
    25981 cgaagatgag cccacccttg tcatgggctt gcaagacgtc ctcgaattcg aaggctatga
    26041 tgtcgtcgtt gcgcccactg gacgcgaggg agtgagtctt gccaaccttc gaaagcccga
    26101 tgtcgtctta ctcgacctga tgcttccgga catcaacggc taccaggtat gcgaagaaat
    26161 ccgcagacgc gatccgttca tgcccatcat catgctcact gcgcgcagcc aagaagcgga
    26221 taaagttcgg ggactcgacg ccggagccga cgactacgtc accaaacctt tcagcatcgc
    26281 ggaactcacc gcaagaatcc gcgccatcgt tcgtcgcgcc tccaggcttc ccgacgccac
    26341 ccctgaattc atcgaaattg gcaacgccaa agtcgatgtg accgcgcaaa c
//
LOCUS       DAOSOR010000007        26294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_1857_length_26294_cov_4.843706, whole genome shotgun sequence.
ACCESSION   DAOSOR010000007 DAOSOR010000000
VERSION     DAOSOR010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 26294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26294
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1857_length_26294_cov_4.843706"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PK710_00770"
     CDS             <1..186
                     /locus_tag="PK710_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HOT08264.1"
                     /translation="VSVRFVIGRDGAVSQVGNAGSDLPSPEVVQCVTSAFYGLSFPQP
                     DGGIVTVIYPILFTPGS"
     gene            277..1623
                     /locus_tag="PK710_00775"
     CDS             277..1623
                     /locus_tag="PK710_00775"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HOT08265.1"
                     /translation="MHRLARTILSVWLVAIALLVARDGHAHPGDRYTISLITMSPGDP
                     IFFRFGHNAILVRDSLRRTHQVYNWGTFSFQEEGLITKFLQGRLTYWLSVQPLGPTLA
                     HYDSEHRWLVEQELNLTPEQKLQLVRKIEENARKENREYRYHYYRDNCSTRVRDVLDS
                     VLDGKLKAISHQPATLTYRGQTSRLVADLEWGRFLLNLAMGNYIDQPITEWEELFVPE
                     KVQQMVRKVETVDATGQTVPLVRVERTLVEAPHRKPPPDMPPNRTWSWLIVGLLGGLL
                     FAALGYRLVSYPAEESAKPSVGRRLALAIPLGAWSTVTGFLGLIFVFFWTMTDHEVAY
                     HNENLLQANPLTAAFPVISVGLMLGKPWALRAFKTLAYVVASLSLFGLMAKALPWFTQ
                     VNGDSIALFLPVWFGLAAGPIVHQIRNKRIARPKDASAPDSSGGPQDVGTQHSPKA"
     gene            1641..2462
                     /locus_tag="PK710_00780"
     CDS             1641..2462
                     /locus_tag="PK710_00780"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT08266.1"
                     /translation="MHWAHRISWIIVVVVIVLPANVFAQVPENVRTVIAQLSSQEQQK
                     VRQGYLALAALDTSAAEACLPTANRVLARGLPVPLAITAIRALGDVGHVTSSRSIRAY
                     LMHRHVEVRREAVNALGKTGGPDARAGLRRALSDADASVRGFAASGLGQVGAKEHVGA
                     LFSALDQGVQEAAASIGMLCSPAECEGLVARADRMGLEVVSPGLREILLRPTTEVTEN
                     IQLSIIERISQGNPRKAQPLLKDVQSRRGRTGSRRVNQAIDLAVRATRPAPEENR"
     gene            2459..4213
                     /locus_tag="PK710_00785"
     CDS             2459..4213
                     /locus_tag="PK710_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08267.1"
                     /translation="MKQAFRLVAFTLLPGLVGCAGGTHSIVFGDIENSSEANPLSVVQ
                     QHLSRHGIANGANVVVGVMDRGIVGLGLDSHHKWFYPHPVNGGVWITGQIVVAGDGKR
                     FTAMEAATGNVVWEQKGLGQIRGASDDGQSTLVWLTSGPKTPGILQTIDRRGHVHRTI
                     DVPEDVGIPAMVGNVGFVPWRNRFVSLVDFEGPKEIARFDQGYPTALAWVMDQAVYVG
                     RDVFTRYDDRITTTGIGRATTKEIPKQQLVDDPTWFPVAGIAGPITREPIENTKIFAK
                     PTPQGATAGYDGQRYYASNRRIVLGLRDDTGELVWVRRLEANILAGAAFEGGVVLCDR
                     AGNVQSFEGKTGALAEQVSLGTALRACVIQVDRFSKPAAPRPKPLVEQLSEAIDGADA
                     SGVKMQGMLVRELGKQPSALATERLIDLAFDSKTLPELMVEVRDGISGRRNGQEAMLA
                     ALSRRYDYLAGVLMTPPVGPLADALAAMKEGRAAPLLAEHLNDPANRTDDVKRAALAL
                     EVLATEAEREPLETFFALYRATAYDDDMTQAVVSVARTLIRIAGDEGKELVRAAAYDP
                     MTVPPLQQPLRRLVSEGS"
     gene            complement(4252..5160)
                     /locus_tag="PK710_00790"
     CDS             complement(4252..5160)
                     /locus_tag="PK710_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT08268.1"
                     /translation="MSLHQARKVFVTGPGGNRLACRILGAHHHRTVVLTSGIGCGPVF
                     MTAIAEELARDHRVVYWDYRSHGESDLAPPTGGYSIAEHADDLDKIVRAFVPSDQKPI
                     MIGFSMGVQVTIEWSRICPDRAGAYVFMLGVPRNPMHRTVLLRSRAAWLAERIGKYGR
                     PVLRRVQPLVNGALRTPLTYEAAKRFGFIARDCPRDEFLDFVHYATSVPLDAYLRCAG
                     GILDHDATDTFLRISQPVFMLAAEDDVLIRAEDARRFADKLPQARFEQLSFASHAGSI
                     EYGTYFAGATRRFLRRLALDQKLRAA"
     gene            complement(5605..6633)
                     /locus_tag="PK710_00795"
     CDS             complement(5605..6633)
                     /locus_tag="PK710_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT08269.1"
                     /translation="MSRYSIVCVLLCLAAISTLWLPSPSSSLPSPASQERTNVRIGLV
                     FDVGGRGDKSFNDSAFAGLERAERELGVTVEMIEPSGAEDREAAMRLFAARGFDLVVG
                     VGFIFSSDILVVANAFASTRFACVDFAPSADQPLPSNFAGLVFREEEGAFLVGAVAGL
                     MSKTKAVGFVGGMNVPLIRKFETGYSSGVRHVCPACRVLSAYAGTTPEAFKDPAKGEA
                     IATSQISAGADVVFHAAGSTGLGVFEAARDMNAWAIGVDSDQYDEMPGTVITSMVKRV
                     DVAVYEVAKEIAEGRFHSGIHTLGLRESGIGYVNDGPHASLLPQDVKTRVDSIRDQVI
                     AGTLRIDP"
     gene            complement(6641..7348)
                     /locus_tag="PK710_00800"
     CDS             complement(6641..7348)
                     /locus_tag="PK710_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08270.1"
                     /translation="MQRRKILLGMTWLCGISPLTVALPSAFAQTPPRILVDRVVARFE
                     AAELGGPAHPRFIFERTLAFEARLEAFAEQQRGVPRPARGYSDRDRRAAIERHVTEEI
                     LVHLPVEPPLRPDEVRRRVIAAYVSLEQLVGGRQSLLDAAYAEGMEMEDLNALVERRA
                     RASLYLDRMVTPMLNPSDAELREVLRTESTPFRHRRFDDIATPLRQWYVADRLRTALA
                     AFFRSARARIRIIMLHP"
     gene            complement(7357..8163)
                     /locus_tag="PK710_00805"
     CDS             complement(7357..8163)
                     /locus_tag="PK710_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HOT08271.1"
                     /translation="MEESPNDAPSTSIRKVPVDWEALENAFENNAPEVHSYLQVMTGD
                     VLRVVDGVADPQMHARIASDPKYLRVEAVSSREQYRWMERYIPTVEDPKLRARLEVAI
                     DGKGAFRRFKDVLMAFGPERERWFTYRSERLKTFMEAWLQAHDIQSVPRAPWTSPEEA
                     TEETATEDARRGHTETVDFRRMRSVEGLRKRMRELSDILGPRDLDTIVSFAEFLKARR
                     SARSFAHHHSFFQSRREEVDEPAEAVRADQENSTAQSFPTDDEVIDSSSP"
     gene            complement(8334..9821)
                     /gene="glpK"
                     /locus_tag="PK710_00810"
     CDS             complement(8334..9821)
                     /gene="glpK"
                     /locus_tag="PK710_00810"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOT08272.1"
                     /translation="MPHFILAIDQGTTGSTALVMDTDGKTLGRANVEFPQHYPQPGWV
                     EHEPDDIWNSVVSAIGKALDQASILGHQIEAIGITNQRETTVVWNRSNGMPIHRAIVW
                     QCRRTAQRCATLKQQGNQDRVRQQTGLVIDPYFSGTKITWLLDEVAGARKQADQGQLA
                     FGTIDSYLTYRLTGGRVHVTDVSNASRTLLMNLHDLRWDESMCELFDVPPSVLPTIVG
                     SAERVGETHGVPGLPDGIPITGMAGDQHAALFGQQCLDVGDVKCTYGTGAFVLVNTGN
                     RPVPSRFGLLTTVGWKIGADIVYALEGSAFIAGAAVQWLRDGLGLIRSASEIESLARK
                     VPSSDGVVLVPALAGLGAPYWNADARGLITGITRGTTAAHLAYATLEGIAHEVSDLLE
                     AMRDDMGKPMGRIRVDGAAAANDLLMQMQADLAGIIVERPKELETTARGAAMLAAIGL
                     GVIDSPKKASGMFMVNRVFQVEQTKEQRDKRRNAWSQAVRRAQTT"
     gene            9992..11827
                     /locus_tag="PK710_00815"
     CDS             9992..11827
                     /locus_tag="PK710_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOT08273.1"
                     /translation="MACLILMTAEGRRTFELRLTNSIGRHPNNTIQLLDRIVSKEHCI
                     VEQRTDGFWLRDLGSLNGTYINAERVNGEQALKHRDEIVMGSTRLWFDDGRGISSNEL
                     APPLASVREESLSPLPSGAMAAVPYHGFAPQPAPALRPSSPVVAAPASGVGLGVSPRA
                     IGTMAIPAFGAEGLQLESLGRIDVMDEKRAIGTQIAATDKGFRAFELVAADPMQLRAD
                     YERLRLSHELSREIALEHDTTKLLGKILQSIFKFVRADRGVIFLKSDDGKLEPGASLR
                     RDGSAAPIVVSSTILNHVEKEKAAVLTHDAAMDFAASKGKSMILNRISSAIVVPLLHN
                     DNFLGVLWLDSESLAQFQPKDLEIVTGVAAQAAMFIENNALAKKIEQELVTRERFSRL
                     LSPNVAERVISGELEVKKGGELVQECTVFNSDIRGFTRMSAKEKPEVIVEMLNEYFEL
                     MVETLFKYEGTLDKFMGDGLMALWGAPIAHPDDPVRAVECALAQMQILGQFNREREQR
                     GLPRLGIGIGIHTGPLVAGYIGSSRALSYTVIGDTVNTSARLCSHAKSGQVVVSQATH
                     DRLGGRFEFEPLEPAKVKGKEAPLAIFNVLRRAPSVPISRETSGS"
     gene            complement(11856..13265)
                     /locus_tag="PK710_00820"
     CDS             complement(11856..13265)
                     /locus_tag="PK710_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08274.1"
                     /translation="MTARITFLHVSLLVLARLAMTAVVGLFGFDAVSDDDFARVVISQ
                     GFASTPKLDPSATSWLPFPFWLTGTAMILTKPTVVVAKILSVVLACGSGLLVYGAARL
                     CKITPESALLGTLGWTVVPTGVLLGAATVPELPTAALCASALLLLRRSDIASAFMASA
                     LVFPATLSRYEAWPVAFCVAAATLRNAWKSDATRGALAASLGVACLACLGPLSWMGWN
                     HVAHGDALHFHARVSSYWFAWGGASNIVDFWLTFAKPLAVDAIVLAVFVAAVAYNARH
                     SPKIITWWVPAISSVVVVAALIAAQCAGAAPTHHPDRSLLFLWAVGWMAVMDRGLTAK
                     PLRYTLFAIVLLVSVARTMILASNYGVHREDERRVGGWLRDHTQGRVLLVPKDYGYFA
                     TLAEMGPSDRLDLTCSLDPRDRSQPCRTETDNELRDRMRTRQARWLVVSGEARAAART
                     LGEARFEVGDWMVVEYNDP"
     assembly_gap    13460..13559
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13659..15746
                     /locus_tag="PK710_00825"
     CDS             13659..15746
                     /locus_tag="PK710_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOT08275.1"
                     /translation="MANGFEPQKIRNVVVVGHKGVGKTTLIEGMLYVGGSTPAMGSIA
                     TKTSVLDDEPEEITREGTLSTSVGHVVWNDTKINIIDTPGEGSFFGDTRLAMAAADAV
                     ILVVSGKDGVQPMTDRLNHWAIEMGLPRFLVFSKCDLEHIHLEPVIESVKKRLKLSLA
                     HMQVTVGEGSDLKGLVDLLGRKVYVGTPDGPKTKASDAIPEEVKDVVEEARMRLVDEI
                     ASTDDELTEKYLDQGDLSDKEMEAGMHKAISSGSLVPLYYFAGGSPYGVIPLLDAIVD
                     YFPPATTRTEVRSKGQEPEIREAKPEAALTAFCFKTTIDQHAGRNAIVRILSGTLKSD
                     AYILNGDSKERVGGIFNVVGKPAGSALGEAYAGDIVSIAKLKTVKTGDTITEDKKPFV
                     FDAPEVPPALFARVVILSDRGSGEKIAQGLSKLSEEDNSLKVYHDEQTHDLICAGLSQ
                     LHLDVTGERLRRRFGTDITLGPPKIPYLETITRKVTNIEGKHKKQTGGHGQFGVCLID
                     MEPTSRGEGFVFDDAIVGGAIPRNFIPSVEKGIVKSAARGFLAGFQVVDFKVRLFDGK
                     YHDVDSSDAAFQLAGSKAFKAAMAKAGPILLEPMVKMEITVPTAALGDIMGDLNSRRG
                     RILGSETQDQFSVVNAQAPLVEVLEYEPKLRALTQGTGTYSMTMDRYEPVPAQLQEKI
                     IKDSGFKAIDEEE"
     gene            complement(15972..17366)
                     /locus_tag="PK710_00830"
     CDS             complement(15972..17366)
                     /locus_tag="PK710_00830"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT08276.1"
                     /translation="MTQPRTSSTTPEPFHRSFWTANATELLERGAYYAMASFVVIYLG
                     RLGMGNYWPSTLNSLLWTLVYFLPILSGTIADHIGFRKALLIAFVLLTAGYLLMGAPV
                     WIGGAQLAEKATDEVTAPIWIGLCVAGAILLIGLGGSVVKPCISGTVQKTAGQRATLA
                     FAIFYMVINIGSLFGRGAAYVVRTHSTLVSIFAVAAACSTAAFLTVLFVFRDPEKDLG
                     ASASDKPKRSVGRILLDMLLVLKNVRFSLFLLANSGFTFLYAQVYNVTPLYLEKVLEH
                     KPPVDIYTMANPFTIVFFQLAITKMFGKIKPIVSIVIGTLLIGASMIINLIPIFVQGG
                     PRITVANLLPLGSLFIVLTVALVAFGELFAQARTYEYIGALAPKGQEGLFLGYANLPM
                     AIGALVGGPVGAFLFNEIMCKNAVKQEDGLLHLDTTYASLGWGILMGVGLFSAAMMWL
                     YNRWVTHSPQPSHN"
     gene            17604..18620
                     /locus_tag="PK710_00835"
     CDS             17604..18620
                     /locus_tag="PK710_00835"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOT08277.1"
                     /translation="MRYRALLVTVVACGVLFTMGPVHAQKKKAAPTTEDPVTLAAREA
                     FIAGTSAVKAGQWSEALVHFEKADQLKPAPVVTFNIGYCQRALGRYILARSSFRKVLS
                     DPSGLPEAQLEEARALLESIETQVLARVTITLLPSSARIAIDGRPLLPYEGGEPGVLV
                     AGVAPAGEGTPPPSATFEVVIDPGVHLIQASRPGHEDVLVNKTFAPNARERLKLELDE
                     LPATIHVESDQPRAVVMVDGHDVGLAPVDLTRPAGTYRVQVVKKGFMTYESALEVAPG
                     QKANLRAKLVVETEPMTKKWWFWGGAAAIIAGGVTATYFLTRPEVQPPAYQGGTLDWV
                     ALPR"
     gene            complement(18636..20456)
                     /locus_tag="PK710_00840"
     CDS             complement(18636..20456)
                     /locus_tag="PK710_00840"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOT08278.1"
                     /translation="MRTAPVLAFAALVLSFSGSAAAQPTDDVPQASPSSQEAPDNERA
                     EPSPHRRVRRLRLPPFTRSVRGPVFTPAPLAPTHGSPPASEPEPNTQPPPVSADPSFP
                     MTTDDALSHGGKPPTQAFEFGDPSVGVDGLPLAGYHDGRFYLRNRTDYFRLFLRSRLH
                     LDAHSAFGPGVEHTDLKPTLLLRRARIELAGEIMGHWQWELQFEAGPTAFNNASGTTE
                     ASAAPAGVQPTAETAAYAPVQTAGYSTRPLNAYVNYRASDHFNIKIGQFNIPFTAENR
                     TSTNNISFLERAMPTRAWGAPETQDIGAMVWGHLDQRTLFWSWAVLQGDGPNRPNADS
                     RAMTAARVYTRPLATEKGPLQLLQIGASFKYAMHDKNFVAYDYPSMGTQSGYRFWTPS
                     YTDSVGMGRKIHVIPSGAQIGVAGELRVPFDRWDLRSEVVYLNNNTREGVDGFQKQYT
                     ERFGTMKGVAYHVTASYWLLGKPFFPGIVGDRQPSRLQLDKPDPGVPPHGVELALKWE
                     QLIANYQSAARSGQVDENNIDGDIRVNAVSAAASYWASKHLRVSLNYVLNHFPEASAT
                     GSSEQRAKAPGNRIAPGLDNEARQSATTLHEVAARIAVAF"
     gene            20624..21655
                     /locus_tag="PK710_00845"
     CDS             20624..21655
                     /locus_tag="PK710_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOT08279.1"
                     /translation="MTRSSLSNVHSHSATHVRAEVEVEDTSVLLALAGASEATFKAIA
                     AELDVEIGLRGTVIHVSGQGDRVALVERFLLEAVDTLSSGIRLTTADMIRGLRFMRRD
                     GSLSLREMFTDLLVVTPNKRTISPKTPNQKRYLQSIRTHDLTFGIGPAGTGKTYLAMA
                     MAVQSLAEHKVKRIILARPAVEAGEKLGFLPGDLAEKVNPYLRPLYDALHDMMDFEKA
                     QALIQRGQIEVAPLAFMRGRTLNDCFVILDEAQNATSDQMRMFLTRLGHASKAVVTGD
                     ITQVDLPSGHCSGLVEARSLLGHIEGISFCTFTEVDVVRHPLVQKIILAYESRDKAQK
                     REAEPNHEP"
     gene            complement(21709..23859)
                     /locus_tag="PK710_00850"
     CDS             complement(21709..23859)
                     /locus_tag="PK710_00850"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOT08280.1"
                     /translation="MDSLVRTILLARSALRHGLGVILALALSVFLVTDLTWNFDPRFR
                     YPAALLFAVAFGASARARYRQSDLVSSRGWQETELGMFSVLGLLGLAAYVDGNLDGST
                     YPVMFIFLVLLSVLAVPWATAVTVGFAISVEAAVRFIAFGEDALRSLLLHGVLASAFS
                     LVGTIVIRAEVARIRTHSRNRLDAEIERMHSAARSYRLLGATARVDDLSDENTPPSSD
                     LDANRLARSSVEEIHQAVLFALQLLHRSLGLHTAMLLWLSDASPHLRISELSSDADNL
                     LEGPFPMNDGIFGAVVTEKNTLTLSNLKASYSLPYYTGACPVRALAAVPVMENDQLRG
                     LLVTDRKDNVAFSSSEQDLLLAATRYMLRAIQNERVFVQLERAKVEQGKLYRAARALG
                     SAMSERDVVEAGVRSAREIASFDFAAVTLFDEAKKMHVIRAVSGDSVQGLVGVKFRHN
                     AGLVSMVVQNKHPLPYRGHYDPNRQVVFAPRVAPAPMSSLLVLPLLVHERPLGTLVLG
                     ARRPGALGEAVRPTLEVLASHVAVSLSGARMMKRLEELATRDGLTGLFNKRALLEMSQ
                     DKLAAAKRFSRKLSLLVVDIDHFKKVNDTYGHDQGDVVLKGLGVILRKAKRTTDAVAR
                     FGGEEFIILCEETDREGAMLLAERIREELQQTTFQTPKGELSVTCSIGIATFPDAGTD
                     WDTLFKAADEALYVSKRSGRNRCTAWSSRRGKAA"
     gene            24096..24908
                     /locus_tag="PK710_00855"
     CDS             24096..24908
                     /locus_tag="PK710_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08281.1"
                     /translation="MVSKKTLESRLAILRHIAESEAIQQVKGRIRDGASWVADQEMVP
                     RVIAGSAGILAALLSDTDPRETQREGIDDVSAVSFDADTPIPSIHPSVEGYWDEQGAE
                     GVEMDDGEGLAAVRDLEGQADLQTPPDTQVSEEEAEEHRLPFEGHEEEDWVAESASAP
                     SAASTARKDKPSKDSRAKDKPKKAAKKSPAKTTKSANAARGAEKKAANRRKQKDVAKP
                     APAEGSEPSSQTAVSPPAKRKPTARQSVVKRPTERGGQKTEPPPKMPTKKST"
     gene            24979..25051
                     /locus_tag="PK710_00860"
     tRNA            24979..25051
                     /locus_tag="PK710_00860"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25012..25014,aa:Ala,seq:ggc)
     gene            complement(25113..25412)
                     /locus_tag="PK710_00865"
     CDS             complement(25113..25412)
                     /locus_tag="PK710_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08282.1"
                     /translation="MSDLEPDRDQEPERESMHLDDDEVRSLLRKAMRVEPRTTASVLP
                     EVQRKIRQRSRGKFFADGWSTASSPSSTYIVTSIVMLVVLAIMYWALVPGGWGTP"
     gene            complement(25409..26014)
                     /locus_tag="PK710_00870"
     CDS             complement(25409..26014)
                     /locus_tag="PK710_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT08283.1"
                     /translation="MSWSEPDEKTFVARLQARDERAFNDLVHHFERRVFALVFRMLGN
                     RVEAEDLTQEVFVQVFKAIDQFRGDSKLSTWIFRIAVNLCKNRNKYLKRRHSNRQDDI
                     DSLGDRSAMNQAKGTTSGSIDRPDDMLVGRQLERIVQLAIQQLEEDFREALVLRDVED
                     MSYEEISEITGLPIGTVKSRIHRARERLREAIESMLGEKVS"
BASE COUNT         5653 a         6865 c         8186 g         5490 t
ORIGIN      
        1 gtatcggttc gcttcgtgat tggaagggat ggtgccgtat cccaggtcgg aaatgccggc
       61 tccgacttgc cgagccccga ggtcgtgcaa tgcgtaacct cagcatttta cgggctttcg
      121 tttccccaac ccgatggcgg tatcgttaca gtcatttatc ccatcctgtt cacgcccggc
      181 agctaacctt tcgtatcgtc acatccacct gtccccccag ttgcgcgatt tcgaaggttg
      241 gcgacccgcc gctcgtcggg gtaagtgttt ctcacgatgc atcgtctcgc acgcaccatc
      301 ttgtcggtct ggcttgtcgc catcgcgctg ctcgtggctc gtgatggcca tgcacacccg
      361 ggggatcgct acaccatctc cctgattacg atgagccctg gagacccgat tttttttcgg
      421 ttcgggcaca acgccattct cgttcgcgac tccttgcgcc gaacccacca agtctacaac
      481 tggggcacct tttcttttca agaagaagga ctcatcacca agttccttca aggccgtctg
      541 acctattggt tgtccgtgca gcccttgggt ccgacgttgg cacattacga ttcggaacat
      601 cgttggctgg tggagcaaga actcaacctc acgccggagc aaaagctgca acttgttaga
      661 aagattgagg aaaacgcgcg gaaggaaaac cgtgagtatc gttatcacta ctatcgggac
      721 aactgttcca cgcgcgtgcg cgatgtgctc gattcggtgc ttgatgggaa gctcaaggca
      781 atatcccacc agcccgccac cttgacctac cggggccaaa cctcgcggct cgtggccgat
      841 ctggaatggg gacgcttttt gttgaacttg gcgatgggca actacatcga tcagcccatt
      901 accgaatggg aggagttgtt cgtcccggaa aaagttcaac agatggtgcg taaggtggag
      961 acggtcgatg caacgggtca gacggttcct ttggtgcggg tcgagcgaac cctggtggaa
     1021 gcaccccacc gaaagccgcc tccggacatg ccgccgaatc gtacatggtc atggttgatc
     1081 gtcgggctgt tgggcgggct gcttttcgcc gccttgggct atcgattggt atcgtatccc
     1141 gcagaggaat cagcaaaacc gtctgtgggt cgaaggctag cgctggcgat ccccctgggg
     1201 gcatggtcca ccgtaacggg gtttttgggg ctgatattcg tgtttttttg gacgatgacc
     1261 gatcatgaag tggcctatca caatgagaat ctgctgcagg ccaatccgct aaccgccgcg
     1321 tttccagtca tttccgtggg tttgatgctc ggcaagccat gggccctgcg tgcttttaaa
     1381 accttggcgt acgtggtggc atcgctatcc ctatttggat tgatggcgaa ggctctgcct
     1441 tggttcacgc aggtcaatgg ggatagtatc gctctattct tgccggtctg gtttgggttg
     1501 gcggcaggac cgatcgtgca tcagattcgc aacaaacgca tcgctcgacc caaggacgca
     1561 tcggcgccgg actcttctgg cggaccccag gacgtgggca cacaacactc cccgaaggcc
     1621 tagagacagg aaacatgtcg atgcattggg ctcaccgaat ctcttggatc atcgttgttg
     1681 tcgtgattgt ccttcccgcc aacgtctttg cgcaagtccc tgaaaacgtc cgcaccgtta
     1741 tcgcccaact ttcgagccaa gaacaacaaa aggtgcggca ggggtatctg gcgctagccg
     1801 cgttggatac gtcggcggcg gaggcgtgcc tacccacggc gaatcgagtt cttgcacggg
     1861 ggcttcctgt gccgttggcg atcacggcca ttcgagcctt gggtgacgtg gggcacgtga
     1921 cgtcttctcg gtcgatccgt gcttacctca tgcatcggca tgtggaggta cgacgggagg
     1981 ccgtgaatgc tttgggaaag acagggggcc ccgatgcgag ggcgggttta cgacgcgcgt
     2041 tgtcggatgc ggatgcttct gtccggggat tcgctgcctc cggccttggg caagtgggag
     2101 ccaaagagca tgtgggtgcg cttttttcgg ctctcgatca aggcgtacaa gaggcagcag
     2161 cgtcgattgg aatgctatgt tcacccgccg aatgtgaggg tcttgtcgcg cgcgcagacc
     2221 gcatggggct cgaggtcgtt tcgccggggc ttcgggaaat cctgcttcgc cccaccacgg
     2281 aagtgactga aaatattcag ctttccatca tcgaacgaat ctcccaaggg aacccgcgga
     2341 aggcccaacc cctgttgaaa gacgtgcaaa gtcgtcgggg tcgtacagga tcgagacgcg
     2401 taaaccaggc cattgatctg gccgtgcgag ccacgcgacc ggcccctgag gaaaatcgat
     2461 gaaacaagcg tttcgcttgg tggcgttcac actgcttccg gggctggtcg gatgcgccgg
     2521 tggaacccac tccatcgttt tcggggacat agaaaatagc agcgaagcca atccactctc
     2581 tgtcgtccaa caacatcttt cgcggcacgg catcgccaac ggagccaacg tcgtcgtggg
     2641 ggtgatggac cgcggaatcg ttggccttgg cctcgactcc catcacaagt ggttctatcc
     2701 gcatccggtg aatggggggg tgtggattac cgggcagatc gttgtggctg gcgacggcaa
     2761 acggttcacc gccatggagg cggcaaccgg aaacgtagta tgggaacaga aagggcttgg
     2821 gcagatccgt ggcgcaagcg atgatggtca gtcgaccttg gtgtggctga cctctgggcc
     2881 caagacaccg ggaatattgc agacgatcga tcgacgaggg cacgtgcatc ggacgatcga
     2941 tgtgccggaa gacgtgggaa ttccggcgat ggtgggcaac gtgggatttg ttccttggcg
     3001 gaatcgattc gttagtttgg tggactttga agggccaaag gaaatcgcgc ggttcgatca
     3061 agggtatccc acggctcttg catgggtcat ggaccaagcg gtttacgtgg ggcgcgacgt
     3121 attcacacgt tacgatgatc gaatcacgac gaccggcatc gggcgagcga ccacgaagga
     3181 gataccgaaa cagcaactcg tggacgatcc aacgtggttt cccgtggcgg gcatcgcggg
     3241 cccaatcaca cgcgaaccta ttgaaaatac taagattttt gcaaaaccca ccccccaagg
     3301 cgccaccgct ggctacgacg gtcagcgata ttacgcttcg aatcgacgta tcgtattggg
     3361 ccttcgggat gacacggggg agttggtttg ggtgcgtcga ctcgaagcca atattttggc
     3421 tggtgcggcc tttgaagggg gcgtggtgtt atgcgatcgc gcggggaatg tgcaatcgtt
     3481 cgaggggaaa acgggggctt tggcagagca ggtttcgttg ggaaccgcgt tgcgggcttg
     3541 tgttatccaa gtggatcggt tttcgaaacc ggctgcacct cgtccaaaac cgctcgtgga
     3601 gcagctttcg gaagcgatcg atggagcgga cgcatcgggg gtgaagatgc aagggatgct
     3661 ggtgcgcgaa ctcggcaaac agccgagcgc tcttgcgacg gagcgattga tcgatcttgc
     3721 ttttgattcg aagacgttgc cggaattgat ggtcgaagta cgtgacggga tatccggtcg
     3781 tcgaaacggg caagaagcga tgctcgcggc gttgtcgcgt cgctatgact atcttgcggg
     3841 agtgctgatg acgcctccgg tggggccctt ggcggatgcg ttggcggcga tgaaagaggg
     3901 ccgtgcggcg ccgctattgg cggagcattt gaatgatccg gccaatcgaa cggacgatgt
     3961 gaaacgggca gcgctggccc tcgaggtact tgcgacggaa gcggagcgag agcctctcga
     4021 gacatttttt gccctgtacc gcgccacggc ctacgacgac gacatgacgc aggcggtggt
     4081 ttcggtggct cgaaccctca ttcgcatcgc gggagacgaa ggcaaggagc ttgttcgtgc
     4141 cgccgcgtat gatccgatga cggttcctcc gttacagcaa cccttacgtc gtctcgtatc
     4201 ggagggatcg taagggcgac tgggcctttc cctgtcgttg gatggtgagg tttacgcggc
     4261 ccggagtttt tggtcgaggg cgagacgacg aagaaagcgt cgtgtggctc ctgcgaagta
     4321 ggttccatac tcgatggatc ccgcgtggct tgcgaaggac agttgttcga atctggcctg
     4381 aggcaacttg tcagcgaatc gccgggcatc ttcggcacgg atgagcacgt cgtcttctgc
     4441 cgcgagcatg aatacgggtt gagagatacg cagaaacgtg tcggttgcat catgatcgag
     4501 gataccgccg gcgcacctta gatacgcatc gaggggaacg gaggttgcgt aatgaacgaa
     4561 gtcgaggaat tcgtcgcggg ggcaatcgcg ggcgatgaag ccaaagcgtt tggcagcttc
     4621 gtaggttaac ggagtgcgaa gggcgccgtt gaccaagggt tgaacgcggc gaagcacggg
     4681 tcttccgtac ttgccgattc tttcggcgag ccaagccgca cgggatcgga gcaacacggt
     4741 acgatgcatg gggttacggg gtactccgag catgaacaca taagctcccg cgcggtctgg
     4801 gcaaatcctc gaccattcga tggtcacctg cacgcccatg gaaaaaccaa tcatgatggg
     4861 cttttggtcg gagggaacga aggctcgcac gattttgtcg aggtcatcgg catgctcggc
     4921 gatggaatat ccgccggtgg ggggggcaag atcgctttcg ccgtggcttc ggtaatccca
     4981 atacacgacg cgatgatctc gagccaattc ctcggcgatg gcggtcatga acacgggacc
     5041 acaaccgatt ccggacgtca gcaccacggt gcggtggtga tgagcaccaa ggatgcggca
     5101 cgcgaggcgg ttgccaccgg ggcctgtaac gaagactttt ctggcttgat gaagggacac
     5161 gccaatacct cgggagggtg aacaagcacg actcgttcca tctgtgtccg tccgcgagtc
     5221 gtgaccttac aaagaacccg attgcgcata attaggcaag aaaccgtcaa taggtgcgaa
     5281 cagtcggtag cacttgctga gaaggacagg taacaacgcg gtcttttcgc gtgcggaggc
     5341 gacgccccaa aaccgtgagc gagcgttggc attcacgagt cgaagctcct accgaagtca
     5401 tgggaaagct aatggagtga tggctcggtc acgggatggt ccgatgctgc tgccccacgg
     5461 catgactggc tccatcgcga acctaggtaa tacaaaatac caattaattc aattagttaa
     5521 gtgcatcatt ccgcggcttg tcgtctacga actatccata aacggatagc tattggcggt
     5581 tggaatcatc ggtcatggca tccttcaagg atcgatacga agggtgccgg cgatcacctg
     5641 atctcggata gaatcgaccc gagtttttac gtcttggggg agtagcgaag cgtgagggcc
     5701 atcgttgacg tatccgattc cgctctctcg aaggcccaag gtgtgaattc cgctatggaa
     5761 tctgccttct gcgatttctt tggccacctc gtagacggcg acatcgactc gtttgaccat
     5821 gctggtgatc acggttccgg gcatttcgtc gtactggtcg gaatcgactc cgatggccca
     5881 tgcattcatg tctctggcag cttcgaaaac accaaggccg gtggatccgg cggcatgaaa
     5941 taccacatcg gcaccggcgc tgatttggct ggtggcgatg gcctctccct tggcggggtc
     6001 tttgaatgct tcgggggtcg tgccagcata cgcggacaga acacgacatg cggggcagac
     6061 gtgtcggact ccggaggaat atccggtctc gaactttcgg atcagcggga cgttcatacc
     6121 gcctacgaat ccgacggctt tggttttgga catcaggcct gctacggcgc cgacgaggaa
     6181 agcgccttct tcttcgcgga acaccaagcc tgcgaagttg gagggcaaag gttggtccgc
     6241 cgagggtgcg aagtccacgc aagcgaaacg ggtagaggca aaggcattgg cgaccacgag
     6301 gatatcggaa gagaagatga atccgacacc aacgaccaaa tcgaaacctc gggccgcgaa
     6361 cagtcgcatg gcggcttccc ggtcctcggc gccactgggt tcgatcattt ccacggtgac
     6421 gcccaactct cgctcggctc gttccaagcc agcgaaggcg gagtcattga agctcttgtc
     6481 gccccggcct cccacgtcga acaccaatcc gatgcgcaca ttggtgcgct cctgcgacgc
     6541 cggggagggg agcgaagatg agggagacgg cagccagagg gtgcttatgg cagccaagca
     6601 gagcaacacg caaacgatcg aatagcgaga catacctggt tcaagggtgg agcatgatga
     6661 tccggatacg cgcacgggca cttcggaaaa aagcggcaag ggccgtccga agcctatcgg
     6721 cgacatacca ttgtcgaagc ggcgttgcga tgtcatcgaa gcgtcgatgg cgaaatggag
     6781 tggattcggt acgcaagact tcgcgcaatt ccgcgtccga cggattgagc atgggtgtca
     6841 ccattcggtc gaggtagaga ctggcgcggg cgcgtcgttc caccaaagcg ttgaggtctt
     6901 ccatttccat accttccgca tacgcggcat cgagcaagct ttggcgtccg ccgacgagtt
     6961 gttcgaggga cacatacgcg gcaatgacac gtcgtcgcac ttcatcgggt cgcaaggggg
     7021 gttcgacggg cagatggact aggatttctt cggtaacgtg tctttcgatg gcggctctac
     7081 gatcgcggtc gctgtagcca cgagcgggtc gcggcacacc tctttgttgt tcggcgaaag
     7141 cttcgagtct ggcttcgaag gcgagggttc gttcgaagat gaagcgtgga tgggcgggtc
     7201 caccaagttc agcggcttcg aagcgtgcga cgacgcgatc gacgaggatt cgtggcgggg
     7261 tttgtgcgaa tgccgaaggg agagccacgg tgagcggtga gatgccgcaa agccaggtca
     7321 ttcccaggag aatctttcga cgttgcacgg gcgggctcac ggtgaggagc tatcgatcac
     7381 ctcatcgtcg gtgggaaagc tttgagccgt ggaattttcc tggtcagcgc gtacggcttc
     7441 cgcgggttca tcgacttctt cgcgacgaga ttggaagaag gagtgatggt gcgcgaagga
     7501 tcgggcactg cgtcgagctt tcaaaaattc ggcgaaagac acgatggtgt cgaggtctcg
     7561 ggggccgagg atatcagaca gttcgcgcat tcgttttcgc aatccttcca cgctacgcat
     7621 gcgacggaag tcgacggttt cggtgtgtcc acgtcgtgcg tcttcggtgg ccgtttcttc
     7681 cgtagcctct tccggtgagg tccaaggggc tcttggtacc gattggatgt cgtgggcttg
     7741 caaccacgct tccatgaacg tcttcaggcg ttcgcttcga taggtgaacc agcgctcccg
     7801 ttcgggacca aaagccatca acacatcctt gaatcttcga aatgcgccct ttccatcgat
     7861 ggccacctcg agacgtgcgc gtagttttgg atcttcgacg gtggggatat accgttccat
     7921 ccagcggtat tgctctctgg aagaaacagc ttcgacacga aggtatttgg ggtcggaagc
     7981 gatccttgca tgcatttgag gatcggcgac accatcgaca acgcgaagta cgtcaccggt
     8041 catcacctgc aagtaggaat gcacttccgg ggcattgttt tcaaaagcgt tttcgagcgc
     8101 ttcccaatca accgggactt tccggatgga tgtgctggga gcgtcgttcg gggactcttc
     8161 catcgctacc tcactaagaa tcgtatggtg cggactgtac tggaaaggga ggccggggaa
     8221 caggcgggat tggaggttgg gtcatatcgt caggccgcat cgggtgatcg tggttttcaa
     8281 tgaaatccac aatcaacgga ttatgcatcc gtggtctgtc cacgaagctg tcatcaagtc
     8341 gtttgggctc gtcggacggc ctggctccaa gcattccgcc ttttgtcccg ttgttctttg
     8401 gtttgttcga cctgaaacac gcgattgacc ataaacattc ccgaggcttt tttgggggaa
     8461 tcgatgacgc cgagcccaat ggccgcgagc atggcagcgc cgcgggcggt ggtttccagt
     8521 tctttgggac gttccacgat gatcccagcc aaatcagcct gcatttgcat gagaaggtca
     8581 ttggccgcgg ccgccccgtc cacccggatg cgccccatgg gtttgcccat atcgtcacgc
     8641 atggcttcga gaaggtcgga gacctcgtga gcgattcctt ccaaggtggc gtaagccaga
     8701 tgagcggccg tggtaccgcg ggtaatgccg gtaataagcc cgcgcgcatc cgcgttccaa
     8761 tagggggcgc ccaatccggc cagggctgga accaagacta caccatcgga actcggtact
     8821 tttcgggcga gcgattcgat ttcggacgcg gaacggatca atccgagccc atcacgcaac
     8881 cattgcacgg cagcgccagc gataaaagcg ctaccttcca gggcgtaaac gatatccgca
     8941 ccaattttcc agccgacggt agtcaaaagc ccgaagcggc tcggtacggg ccgatttcct
     9001 gtattgacaa ggacgaacgc gccggtgcca taggtacatt tgacatcccc cacgtcgagg
     9061 cattgctgtc caaacagggc agcatgctga tccccagcca tcccggtaat cggaattcca
     9121 tcgggcaaac cgggcacgcc atgagtttct cccacccgct cggcggatcc gacgatcgtg
     9181 ggcaacacgg aggggggcac gtcgaacaac tcgcacattg actcatccca ccgaagatcg
     9241 tgcaagttca tgagcaaggt tcgtgaggca ttgctcacat cggtcacgtg gactcgtccg
     9301 ccagtgagtc gatacgtcaa gtagctatcg atggttccga acgccagttg tccttggtcg
     9361 gcttgttttc gagcaccggc gacctcatcg agaagccagg tgattttcgt gcccgaaaag
     9421 tatggatcga tcaccaaacc ggtttgttgt cggacacgat cttgatttcc ctgctgcttg
     9481 agtgtggcgc aacgctgggc tgtccgacgg cactgccaga cgatggcgcg atgaatgggc
     9541 atgccgttcg atcggttcca gacgacggtg gtttcgcgtt ggttggtaat accgatggcc
     9601 tcgatttggt ggccgaggat ggaagcctgg tcgagggctt tgccgatggc ggatacgacg
     9661 ctattccaga tgtcgtcggg ttcgtgttcg acccatccag gttgtggata atgctgagga
     9721 aattcgacat tggctcgtcc gagggttttc ccatcggtgt ccatgacgag ggcggtggat
     9781 ccggtcgtcc cttgatcgat agcgaggatg aaatgcggca tgatgggtcc tttctgcctt
     9841 gtcctaccac gggagaattg ggggagggta gccgcagtca tgggcagggc agatgaaaaa
     9901 ctgtgtattc ttgggtgagt gtcgcgggga tgcgtgccac gtgttgatgg tcgttgtttg
     9961 agaaggaagc agggggtagg ctgccgcgtt gatggcgtgt ctcatcctca tgactgcgga
    10021 gggcaggcgg acgttcgaac ttcgcctcac caactccatc gggcgccacc cgaacaacac
    10081 catccagctt ctggatcgga tcgtttccaa agaacattgc atcgttgaac aacgaaccga
    10141 tgggttttgg cttcgtgatc tcggaagctt gaacggaacc tacatcaatg cggagcgtgt
    10201 caacggcgag caggcgctca agcatcgtga tgagattgtc atgggttcga cacggttatg
    10261 gtttgacgat ggtaggggga tttcttctaa cgagcttgcg ccgcctctgg catcggttcg
    10321 agaggaaagc ttgtctccgc ttccgagcgg agcgatggcg gcggtgcctt atcacgggtt
    10381 tgccccacag cctgcacctg ctttgcgtcc ttcttcgccg gttgtcgcgg ctcccgcgtc
    10441 aggggtcggc ttgggagtat caccccgagc gattgggacg atggcgattc cggcatttgg
    10501 agccgaagga cttcaactcg aatcgttggg ccgcattgac gtcatggacg aaaagcgtgc
    10561 cattggcacc caaattgcgg cgacggacaa ggggtttcga gctttcgagt tggtggcggc
    10621 ggatccgatg cagcttcgag ccgattacga acggcttcga ttgagccacg agttgtctcg
    10681 cgagatcgct ttggaacacg atacgaccaa gcttcttgga aaaatccttc agtcgatctt
    10741 caagttcgtg cgggcggatc ggggtgttat ttttttgaaa tccgacgatg gcaagctcga
    10801 gccgggagcc agtttacgac gtgatggttc ggcggcaccc atcgtggtgt cctcgaccat
    10861 tctcaatcac gtggagaaag agaaggcggc cgtgctcacc cacgatgcgg cgatggactt
    10921 cgccgcgtcg aaaggcaaga gcatgatttt gaatcggatc agctcggcca tcgtggttcc
    10981 tttgctccac aacgacaact ttcttggtgt gctttggctc gactccgagt ccctcgctca
    11041 attccagccc aaagatctcg aaatcgtgac gggggttgcg gctcaagccg ccatgttcat
    11101 cgagaacaac gctctagcga agaagatcga gcaggaactc gttacacgag aacgattcag
    11161 ccgtctgtta tcccccaatg tagccgaacg tgtcatcagc ggtgaactcg aggtaaaaaa
    11221 gggaggcgaa ctcgttcaag aatgtacggt tttcaattcc gatatccgtg ggttcactcg
    11281 tatgagcgcc aaggaaaaac ccgaagtcat tgtcgagatg ctcaacgaat acttcgaact
    11341 catggtcgag actttattca agtacgaggg aacgctcgac aagttcatgg gagacgggct
    11401 gatggccctg tggggcgcac cgatcgctca tcccgatgac cccgtgcgcg ccgtggaatg
    11461 cgctctcgcg cagatgcaaa tcctcgggca gttcaatcgg gaaagagaac agcggggtct
    11521 tcctcggctc ggcattggca tcggcatcca caccggacca ctcgtcgccg gatatatcgg
    11581 aagttcccgc gcactgtcct acaccgtcat cggcgatacg gtaaacacgt cggcgcggct
    11641 atgctcccat gccaaatccg gacaggtggt tgtcagccag gccacccacg atcggcttgg
    11701 cggacgattc gaattcgagc ccttggagcc tgcgaaagtc aaaggcaaag aagctccgct
    11761 cgccatcttc aacgtgttgc gcagggctcc ttcagtgcct atttcccgag agacgtctgg
    11821 ctcttgatat cggtgggggc gtgttggaga gggcgctaag ggtcgttgta ttcgaccacc
    11881 atccaatcac cgacttcaaa ccgagcttcg ccaagggttc gtgccgcggc tctcgcttcg
    11941 ccgctgacga ccagccatcg cgcttgccgc gttcgcattc ggtcgcgcag ttcattgtcg
    12001 gtttcggtac ggcaaggttg tgagcgatct cgcgggtcga gcgagcaagt cagatcgagc
    12061 cggtccgaag ggcccatctc tgcaagagtc gcaaaataac catagtcttt gggaaccaac
    12121 aaaacgcgtc cttgggtgtg atcacgcaac caaccgccaa cacgtcgttc atcctcacga
    12181 tgcactccgt agttcgaggc aagaatcatg gtgcgcgcaa cggataccag gagcacaatc
    12241 gcaaacaggg tgtagcgcag aggtttcgcc gttagccccc tatccatcac ggccatccaa
    12301 cccacggccc aaaggaacaa caacgaacga tcgggatggt gcgtgggagc ggctccagcg
    12361 cattgagccg cgatcaaagc ggctacgacg actacgctcg atatcgccgg tacccaccag
    12421 gtaatgattt ttggactatg gcgcgcgttg tacgccactg cggctacgaa cacggcaagg
    12481 acgatcgcat cgacggccag tggtttggcg aaggtgagcc aaaaatctac gatgttggat
    12541 gcgccacccc aggcaaacca atacgaggag acacgcgcgt gaaagtggag agcatcaccg
    12601 tgggctacgt gattccaccc catccagctc agggggccga ggcaagcaag gcaagcaacg
    12661 ccgagagagg ctgcgagcgc accacgggtg gcatccgatt tccatgcgtt gcgaagggtg
    12721 gccgcggcca cgcaaaacgc gaccggccag gcttcgtagc gagacagcgt cgcggggaaa
    12781 accaaagcgc tggccatgaa agccgaggcg atgtcggaac ggcgaagaag cagtagcgcc
    12841 gaagcgcaca aggcagccgt gggcaattcg gggactgtcg cggcacccaa aagcacgccc
    12901 gtcggcacaa ccgtccaccc caatgttcct aacagtgcgg attctggggt gattttgcaa
    12961 agccgggcgg caccatagac aagcaacccc gaaccacatg ccaagacaac cgagagtatt
    13021 ttggccacga ccacggtcgg tttggtcagg atcatggcgg tccctgtgag ccaaaacgga
    13081 aagggcagcc aactggttgc gctggggtcg agtttgggtg tcgatgcaaa tccttgtgaa
    13141 atcaccaccc gagcgaaatc atcatcggac acggcatcga agccgaacag ccctacgacc
    13201 gccgtcatcg caagccttgc caggacaagc agcgatacgt gtagaaacgt tattcgggcg
    13261 gtcatggcag tggcactaca ccggcgaagg gtcagggggg aatgcgcaag atattacgca
    13321 ttgcgtcaaa tagcggtggt agcaggtatg aaaacggctt gggggaggca tggggcaaaa
    13381 attcgtacgg tgtcgtggac tggggtgcca tggtccgcta gctttcgcgc ctcgcttgat
    13441 taggataccg ccttccgtcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
    13561 tgccatggtc cgctagcttt cgcgcctcgc ttgattagga taccgccttc cgtcctccgg
    13621 ggtcccggag aaggattcat tgtaaggaga gcaggaacat ggccaacggg ttcgaacccc
    13681 agaagatccg aaacgttgtc gtggtaggtc acaagggcgt tgggaagacg acgctcatcg
    13741 agggcatgct gtacgtgggg ggctcgactc ctgcgatggg gagcatcgcg accaagacga
    13801 gcgttttgga tgatgagccc gaggagatta cgcgcgaggg gacgctttcg accagcgtag
    13861 gccacgtggt atggaacgac acgaagatca acatcatcga cacgccagga gagggatcgt
    13921 tttttggtga tacgcgtctt gccatggcgg cggcggatgc ggtgattctc gtagtttcgg
    13981 gcaaagacgg tgtgcagccg atgacagatc gtctgaatca ttgggcgatc gaaatggggt
    14041 tgcctcggtt ccttgttttt tcgaagtgcg atctcgagca tatccatctc gagccggtca
    14101 tcgaatccgt gaagaagcgt ttgaagcttt cgcttgcgca catgcaagtg acggtgggtg
    14161 aaggttcgga tctcaagggg ttggtcgatt tgctgggccg caaggtttat gtgggcacac
    14221 cggatgggcc gaaaaccaaa gcaagcgatg cgatcccgga agaggtcaag gatgtggtgg
    14281 aggaagctcg gatgcgtttg gtggatgaga ttgcgtccac ggatgatgag ctgacggaga
    14341 agtacttgga tcaaggcgat ttatccgaca aggaaatgga agcgggcatg cacaaggcca
    14401 tttcttccgg gtcgctcgtt cctctttact actttgcggg aggttcccct tacggtgtca
    14461 ttccgttgct ggatgcgatt gtcgattact ttccgcctgc gacgacgcgt accgaggtgc
    14521 ggagcaaggg gcaagagccg gagattcgag aggccaagcc ggaggctgct ctgacggcgt
    14581 tttgcttcaa aaccacgatt gatcagcacg cgggtagaaa tgccatcgtg cgtattcttt
    14641 cgggtacgct caagagcgac gcctacattc tcaatggtga ctccaaggag cgggtgggtg
    14701 gaattttcaa tgtggtgggc aagcctgcgg gaagtgcgtt gggtgaagcg tatgcgggag
    14761 acatcgtatc catcgccaag ctcaagacgg tgaaaacggg cgatacgatt acggaagaca
    14821 agaagccgtt cgttttcgac gcgcccgagg ttccgcccgc cctatttgcc cgtgtcgtca
    14881 ttctcagcga tcgtggtagt ggagagaaga tagcccaggg gctttccaaa ctctccgaag
    14941 aagacaactc gctgaaagtc tatcatgatg agcaaaccca cgacctcatc tgtgctggcc
    15001 tcagccagct tcatctcgac gtgacgggcg agcgtctgcg tcgtcgtttt ggaacggaca
    15061 tcacccttgg tccgcccaag atcccctacc tcgaaaccat cacacgcaag gtgacgaaca
    15121 tcgaagggaa gcataagaag cagaccggtg gtcacggaca gtttggtgta tgcctcattg
    15181 atatggagcc cacgagtcgc ggcgaaggat tcgttttcga cgatgccatc gtcggtggag
    15241 cgattcctcg caacttcatc ccgagtgtcg agaaaggaat cgtcaagagc gcagcgcgag
    15301 gcttcctcgc gggattccag gtggtcgact tcaaggtccg tttattcgac ggcaagtatc
    15361 acgatgtgga ttcctcggat gcagctttcc agcttgctgg ttccaaggcc ttcaaggcgg
    15421 ccatggccaa agcgggtccg atcttgctcg agcctatggt caagatggaa atcactgttc
    15481 cgaccgcggc gctgggcgac atcatgggcg atttgaacag tcgtcgcggc cgaatccttg
    15541 gcagcgaaac acaagatcag ttcagtgtcg tcaatgcgca ggcaccgttg gtcgaggtgc
    15601 tcgaatacga gcccaaattg cgtgcgctta cgcaaggcac ggggacgtat tcgatgacga
    15661 tggaccgtta cgagccggta cctgcccagc tacaggaaaa gatcatcaag gattccgggt
    15721 tcaaggctat cgacgaagaa gagtaacgta tcgtcgctca tgccgttttt ggcgacagtg
    15781 gctttgagca tgacggcttg tccattcttg ggcgagttcg tggttctcct cccttgatcc
    15841 agacaaccat tgcttcgggc ttgacggtgc ctgggggagg ggtcccgttt tgggcttggc
    15901 ttttctgaca aggccatgga gggctgaagg ccagcgaggc tcaagtatcc gaaaaaaacc
    15961 tataattacg cttagttatg tgatggctgg ggactgtggg tcacccatcg attgtaaagc
    16021 cacatcatcg cggcggagaa gagacctacg cccatgagga ttccccaccc gaggctcgcg
    16081 taggtcgtat cgaggtggag caagccatcc tcttgtttta ccgcgttttt gcacatgatt
    16141 tcgttgaaca ggaaggctcc gaccggccca ccgaccaagg cgccaatggc catgggcagg
    16201 ttggcatagc ccaggaaaag gccttcttgg cctttggggg cgagagcgcc gatgtactcg
    16261 taggtacgag cttgggcaaa tagttctccg aaggcaacga gagcgacggt caacacgatg
    16321 aacagggagc cgaggggcag gaggttggcg acggtgatgc gggggcctcc ttggacgaag
    16381 atggggatga ggttgatgat catggaggcg ccgatgagca aggtaccgat gacgatcgac
    16441 acgatcggct ttatttttcc gaacattttt gtaatggcga gttggaagaa cacgatggtg
    16501 aacggatttg ccatcgtata gatgtcgacg ggtggtttat gctccaggac tttttcgagg
    16561 tagaggggcg tcacgttata gacttgagcg tacaggaagg tgaacccgga gttcgcgagc
    16621 aaaaacaacg agaaccgcac gtttttcagc accaaaagca tatcgaggag gattcgccct
    16681 acggatcgtt ttggtttgtc cgaggccgag gcacctaggt ctttttcggg gtcacgaaaa
    16741 acgaaaagca cggtgagaaa ggcagcggtc gagcaagcgg cggcgacggc gaaaatggat
    16801 acgagggtgg agtgggttcg caccacataa gcggcgccgc gtccaaaaag cgaaccgatg
    16861 ttgatgacca tgtagaagat ggcgaaggca agggtggcgc gctgtcccgc ggttttttgc
    16921 acggtccctg aaatacaggg ttttacgacc gatcccccca atccgatgag caggatcgcg
    16981 cccgcgacac ataggccgat ccagatggga gcggtcacct catcggttgc tttttcggca
    17041 agttgcgcac ctcctatcca gacgggggca cccatgagca agtagcccgc ggtaagcaat
    17101 acgaaagcga tgagcagagc ttttcgaaag ccgatgtggt ccgcgatcgt tcccgacaag
    17161 atgggcaaga agtacacgag ggtccaaagc aagctattga gggtgctcgg ccaataattc
    17221 cccatgccga ggcgacccaa gtagatcacc acgaacgagg ccatcgcgta atacgcgcct
    17281 cgctccagca gttcggtggc attcgccgtc caaaaactgc gatggaaagg ctccggggtc
    17341 gtggaagacg tgcggggttg ggtcatggcc gtcccatacc attaccgtcg tgtcatgggc
    17401 acgggattac ggaagcgaag cagtgctgcc actggcctca tccaaagggt gtatttcgtt
    17461 ggacggctgg gggactcgtg ggagcaggcc ccaggcaagc ggcacgaatc gatggcggga
    17521 caaggggcgg ccttccaagg ctttcgttgc gaaatgggct tgtgggttgt gaaccgggcc
    17581 agaacagggc attcttatct ttgatgcgtt atcgcgcact tcttgtcacg gtcgtcgctt
    17641 gtggtgtgtt gttcaccatg gggcccgttc atgcccagaa gaaaaaggcg gctcccacca
    17701 cggaagatcc tgtgacgctc gcggctcggg aggcattcat tgcgggaacg agcgcggtca
    17761 aagccggtca atggtcggaa gccctcgttc atttcgaaaa agccgaccaa ctcaagcctg
    17821 ctcccgtcgt gaccttcaac atcgggtatt gtcagcgtgc gctcggacgc tacatcctgg
    17881 cccgatcgtc gtttcgcaaa gtgctatcgg atccatcggg gttacccgag gcacaactcg
    17941 aagaagcccg ggcgttgctc gagagcatag aaacccaggt gcttgctcga gtcacgataa
    18001 cgttgctgcc ttcttcagcg cgcattgcca tcgacgggcg tcctttgctt ccctatgagg
    18061 ggggggaacc gggagtgctc gtcgcgggtg tggcaccggc gggagaaggg acaccaccgc
    18121 cgtcggcgac gttcgaggtg gtgatcgatc cgggggtaca tctcattcag gcatcccgtc
    18181 ctggtcatga ggatgtgttg gtgaacaaga cgtttgcgcc caacgcgagg gagagattga
    18241 agttggagct tgatgagctt cccgcgacga ttcacgtgga gagtgatcag cctcgtgcgg
    18301 tggtgatggt ggatggtcat gacgtgggat tggcgcccgt cgatctgacg cgtcctgcgg
    18361 ggacttaccg tgtgcaggtg gtaaaaaagg ggttcatgac gtacgagtcg gctttggagg
    18421 tggcgcctgg tcaaaaggcc aatcttcgcg cgaagcttgt cgtggaaacg gagccgatga
    18481 cgaaaaaatg gtggttttgg ggtggggcgg cggcgatcat cgctggcggt gtcacggcca
    18541 cgtactttct caccaggccg gaagtgcaac ctccagcgta ccaaggggga acgctcgatt
    18601 gggttgctct tccgcgctga tgggtcaatt gtcgttcaga atgccactgc gatccgtgcg
    18661 gcgacttcgt gcaaggtcgt agcggattgt ctcgcctcat tatcgagtcc cggtgcgatt
    18721 cgatttcctg gagccttggc acgttgttcc gaggaacccg tcgcacttgc ctctggaaag
    18781 tggttcagga cgtagttgag tgatacgcgc aggtgtttgg aagcccaata actcgcggct
    18841 gcggacacgg cattgacgcg gatatcgcca tcgatgttgt tctcatcgac ctgtccgctt
    18901 cgcgccgcgc tttggtaatt ggcgatgagc tgttcccatt tgagtgccaa ttcgacaccg
    18961 tggggcggga ctcccgggtc gggtttgtcg agttgtaagc gtgagggctg tcgatcaccc
    19021 acgatgcccg gaaagaaagg ttttcccaag agccagtagg atgcggtgac gtgataggca
    19081 acgcccttca tcgttccgaa gcgttcggta tattgttttt ggaagccgtc gactccttct
    19141 cgggtgttgt tgttcaagta cacgacttcg gatcgcaggt cccatcgatc gaaggggacc
    19201 cgaagttcgc cggcgacacc gatttgcgct cccgaaggga tgacgtggat tttacgtccc
    19261 ataccaaccg aatccgtata gctgggagtc cagaatcgat atccgctttg cgtgcccatg
    19321 gaggggtagt cgtaggcgac gaagttcttg tcatgcatgg catatttgaa gcttgcgcca
    19381 atttggagca gttgcagagg gcccttttcc gttgcgaggg ggcgcgtata tacccgcgcg
    19441 gcggtcatcg cgcgactatc cgcattggga cgattggggc catcgccttg caagacggcc
    19501 cagctccaaa acagggttcg ttggtcgaga tgaccccaca ccatggctcc gatatcctgg
    19561 gtttctgggg ctccccacgc gcgggtgggc atggcgcgct ccaagaagga aatgttattg
    19621 gtggaggtac ggttctccgc ggtgaagggg atgttgaact gtccgatttt gatattgaaa
    19681 tggtcggagg cgcgatagtt gacgtacgcg ttcaaagggc gagtgctgta gccggcggtc
    19741 tgaacggggg cataggcagc ggtttcggcg gtgggttgga cgccggcggg tgccgcgctg
    19801 gcttcggtgg tgcccgacgc attgttgaag gcggtgggac ctgcttcgaa ttggagttcc
    19861 cattgccaat gccccatgat ttcgccggct agctctatcc gagcacgccg caagagcaag
    19921 gtcggtttca ggtccgtatg ctccacaccc ggtccgaacg cgctgtgggc gtcgagatga
    19981 aggcgagatc gtaaaaacaa acgaaaataa tccgtgcgat ttcgaagata gaagcggcca
    20041 tcgtgatagc ctgccagggg aagtccatcc acgccgacgc ttggatcgcc gaactcgaag
    20101 gcctgtgttg gtggttttcc gccatgggaa agggcatcgt ccgtggtcat cgggaaagag
    20161 ggatcggcgg aaacgggcgg aggttgggtg ttgggctcgg gttccgaagc tggaggcgag
    20221 ccatgcgtcg gagcgagagg cgcaggggta aaaaccggac ctcgtaccga tcgggtaaaa
    20281 ggagggagcc gcaatcgtcg cactctccga tgcggtgatg gctctgctcg ttcgttgtcc
    20341 ggagcctctt gggaagacgg tgaagcctgg gggacgtcgt cggtgggctg ggccgcggcg
    20401 gaaccactga aggacaagac aagcgcagcg aaggcaagca cgggagcagt gcgcatcagg
    20461 gcaacctcct gggaatacga attgtttgcg catcgtgctt ggttgccagc agccgcgtca
    20521 agtatgtcta gcttccgggc ggtcgcttgg ggaaatgggc gcaaaggctt gccaaccaga
    20581 cggagcgtgc gtcggcgcat ttctagcgat aagctcgttt tcaatgacgc gcagctccct
    20641 gtcgaacgta cattctcatt cagccacgca tgtgcgtgcg gaagtcgaag tggaagacac
    20701 ctcggtgttg ctggctctgg cgggggcttc cgaggccact ttcaaagcca ttgctgccga
    20761 gttggacgta gaaatcggcc ttcggggaac ggtcattcat gtttccgggc aaggcgatcg
    20821 agtcgccctc gtggagcgtt ttcttttgga agccgtcgat accctgtctt ctgggattcg
    20881 tttgacgact gcggacatga ttcgggggct tcgtttcatg cgcagggatg ggagtttatc
    20941 gctgcgagag atgtttacgg atcttttggt cgtgacgccc aacaagcgca ccatctcgcc
    21001 caaaactcct aatcaaaagc gatacttaca aagcatacgc acgcatgacc tgacgttcgg
    21061 aattggtcca gcgggcactg ggaaaaccta tctagccatg gccatggcgg tccaatcgtt
    21121 ggctgaacac aaggtcaagc ggatcatcct ggcgcgtccc gccgtggaag cgggagaaaa
    21181 acttgggttt ttaccgggag atctcgcgga gaaagtaaat ccctacttga ggcccttata
    21241 cgatgcgttg cacgacatga tggattttga gaaggcgcaa gcgctgattc aacgaggtca
    21301 gatcgaagtg gcaccgctcg ccttcatgcg tggcagaacg ctcaacgatt gtttcgtcat
    21361 tcttgatgaa gcacagaacg cgacgagcga tcagatgcga atgtttttga cacgtcttgg
    21421 gcatgcttcg aaagcggtgg tgacgggaga catcacgcag gtggatttac cttcgggcca
    21481 ttgcagcggg ctggtggaag cccgttcttt gcttggtcat atcgaaggga ttagcttttg
    21541 cacattcacc gaggtggatg tggtccggca ccctctcgta cagaagatca tccttgccta
    21601 tgagtcacgg gacaaggcgc aaaaacgcga ggcggagcca aaccacgagc cataatcgcg
    21661 gcgcaacgac ctggggtcgg cggttttcga ggctttggag aagagatatc aagcggcttt
    21721 gccgcgtcgg gaagaccagg cggtacaacg atttcggccg gagcgtttgg aaacgtacaa
    21781 ggcttcatcg gctgctttga aaagcgtatc ccaatccgta cccgcgtccg ggaaggtggc
    21841 gatgccgatg gagcaagtca cggacaattc gccttttggc gtttggaagg tcgtttgctg
    21901 aagttcttca cgaattctct cggcaaggag catggctccc tcgcgatccg tttcttcaca
    21961 aaggatgatg aattcctcgc cgccaaaacg cgcgacggcg tcggtcgttc gctttgcttt
    22021 tcgcaaaatg acgcccaatc ccttgagcac gacgtcgccc tgatcgtgac cgtaggtgtc
    22081 gttgactttt ttgaagtggt cgatatccac gacgagcaag cttagttttc gcgagaatct
    22141 ctttgccgcg gccagtttgt cctgggacat ttcgagcaag gcccgtttgt tgaacaatcc
    22201 cgtaaggcca tcacgggtgg cgagttcttc gaggcgtttc atcatacgag cgccggacaa
    22261 ggacacggcg acatgactgg ccagcacttc cagggtggga cggacggcct cgccaagagc
    22321 gccgggtctt cgtgcgccga gcacgagggt gcctaaggga cgttcatgaa cgagcagggg
    22381 aagcacgagc aaggaagaca tgggggcagg ggccacgcgg ggagcgaaaa caacttgtct
    22441 gtttggatcg tagtgacccc gatagggcag cggatgtttg ttttggacga ccatggatac
    22501 gaggcccgcg ttatgtcgaa acttcacccc cacgaggcct tgtacggaat cgccacttac
    22561 cgcgcgaatc acgtgcatct tcttcgcttc atcgaaaagg gtcacggcag cgaagtcgaa
    22621 agaagcgatt tcgcgagcgc tccgcacacc agcttcgaca acgtctcgct cgctcatggc
    22681 ggagccaagg gctcgcgcag cacggtacag ttttccttgt tccactttcg cccgttcgag
    22741 ttgcacgaaa acgcgttcgt tttggatggc acggagcatg tacctcgtag ccgcaagcag
    22801 caggtcttgt tctgaggaag aaaacgccac gttgtctttt cggtcggtca ccaagagccc
    22861 gcgcagttgg tcgttttcca tgacaggaac ggccgcaagg gcccggaccg gacatgctcc
    22921 cgtgtagtag ggaagcgagt aggaagcttt caggttggag agggtaaggg tatttttttc
    22981 cgtgacgacg gcaccaaaga tgccatcgtt catcggaaat ggcccttcga gcaggttgtc
    23041 cgcatcggac gatagttcgc tgatgcgaag gtggggactt gcatccgaaa gccataacag
    23101 catggcggtg tgcaatccca gggatcgatg gagcaactga agagcaaaga gcacggcttg
    23161 atggatttcc tccacgctcg agcgggcgag ccgattcgca tccaagtccg atgatggagg
    23221 ggtattttcg tcactcagat catcgacacg agcggtcgcg ccgagaagac gataggaccg
    23281 cgcggcagaa tgcatgcgtt cgatttccgc atcgagacga ttgcgggaat gggttcggat
    23341 gcgagccact tccgcacgaa tcacgatggt tcccacgaga gaaaacgcag aggctagcac
    23401 cccgtggagc aacaaggatc gaagggcatc ttcaccgaag gcaatgaatc gaacggcggc
    23461 ttccaccgaa atcgcaaagc caacggtgac ggctgtcgcc caaggaaccg caagcaccga
    23521 cagcagaacc aaaaagatga acatgaccgg gtaggtagac ccatcgagat tgccgtcgac
    23581 gtatgccgcg agacctagca aaccaagcac ggagaacatg ccgagttcgg tttcttgcca
    23641 tcctcgcgag gagacgagat cggactgacg ataccgcgcg cgagcggaag ccccgaaagc
    23701 aaccgcaaac agcaaggccg cggggtaccg aaaccgaggg tcgaagttcc acgtcaggtc
    23761 cgtgacaagg aatacggaaa gggccaaagc gaggataacg ccaagaccgt gacgcagggc
    23821 gcttcgtgcg aggagtatcg tgcggacgag cgagtccata cagcttgatg gctcttatca
    23881 gccggggagt cggtcggccc actttgtggc cgactcattt gtaaccaatt gaatttattc
    23941 aatcattatt gatgacggaa agacgaggtc atgagggggg agcgaggcag ggtcaagggg
    24001 gtaggaatag gcggttgacg ccatggccca tcacggtgag acaatagagg cttgtaaagc
    24061 cgtgcgcggc catacccagg tgaaagaagg cgcccgtggt atcgaaaaag acgctggaaa
    24121 gcagattggc aattctccga cacatcgctg aaagtgaggc gatccaacaa gtaaagggtc
    24181 gtattcgcga tggcgcttct tgggtggcgg accaggagat ggtgccacgg gtcatcgcgg
    24241 gttcggcagg tattttggcg gcgttgttga gcgacactga tccacgggag acgcaacgag
    24301 agggcatcga tgatgtttct gccgtcagct tcgatgcgga cacgccgatt ccgtcgattc
    24361 atccgagcgt ggaggggtat tgggacgagc agggagcaga gggggtggag atggacgacg
    24421 gagaaggttt ggcggcggta cgtgatttgg agggtcaagc ggacctacag actccgcctg
    24481 acacccaggt cagcgaagaa gaggcagagg agcatcgtct tccgttcgaa gggcatgagg
    24541 aagaggattg ggtagccgaa agtgcgtcgg ctccatcggc agcatcgaca gcgcggaagg
    24601 acaagccgtc gaaggattcg cgagccaagg acaagccgaa aaaggcggcg aagaaatcac
    24661 cggccaagac caccaagtcc gcgaatgccg cccgcggagc ggaaaagaag gcagccaacc
    24721 gacgaaaaca aaaagacgtg gcaaagccgg ctccggccga ggggtcggag ccatcgtcgc
    24781 aaacggctgt ttctccaccg gcgaaacgca aaccgacggc caggcagagc gtggtgaagc
    24841 ggccgacgga aagagggggg cagaaaacgg agccgccgcc caagatgccc accaaaaaaa
    24901 gtacgtgaca tacgctgggt taacggctag attacgcatc cctgttggcg gttctgtcga
    24961 tggggaagct tccctcatgg ggctgtagct catctgggag agcgtctgac tggcagtcag
    25021 aaggtagaga gttcaagtct cttcagctcc acaacgagcg cgaatggcaa acgattcgcg
    25081 ctcgtatttt tttgtcctcg cgttttttgt gctcaggggg ttccccatcc gcctgggacg
    25141 agcgcccaat acatgatggc aagcacgaca agcatcacga tgctcgtgac gatgtaggtt
    25201 gaggacggcg agctagccgt actccagccg tcggcgaaaa attttccgcg ggagcgttgt
    25261 cgaatcttgc gttggacttc aggcaacaca cttgcggtcg ttcgcggctc tacccgcatg
    25321 gcctttcgca acagcgagcg aacctcgtcg tcatcgagat gcatcgactc acgctcaggc
    25381 tcttggtccc gatccggttc gaggtcgctc atgatacctt ctccccaagc atgctctcga
    25441 tcgcttctcg aagccgttct cgggctcgat gaattcggct tttgaccgta ccgatgggaa
    25501 gccctgtgat ttcgctgatt tcctcgtagc tcatgtcttc gacatcgcgc aggacgagcg
    25561 cctcacggaa gtcttcttcg agctgttgaa tcgctagctg aacgatccgc tcgagttgac
    25621 gacctacgag catgtcatcg ggacgatcga tggagccgga ggtggttcct ttcgcctgat
    25681 tcatggcgga gcgatcgccc aacgaatcga tatcgtcttg tcgattggaa tggcgacgct
    25741 tcaggtattt gtttcggttt ttgcataagt tgacagcgat tcgaaagatc caggtagata
    25801 gtttcgaatc tcctcggaac tgatcgatcg ccttgaagac ttgaacgaag acctcttggg
    25861 tcaagtcctc tgcttcgacg cggttgccaa gcattcggaa aacgagcgcg aagacgcgac
    25921 gttcgaagtg gtgaaccaag tcgttgaagg cacgttcatc tcgcgcctga aggcgcgcta
    25981 cgaaagtctt ttcatcgggt tcgctccaag acaccacgga ctcatcctac gaaagggcgc
    26041 atggtcgact gtcgctggca ccaagaccca aggtggttcc aacagaatgg gagaaagaag
    26101 tccatgcggt cgaggatatc tcctgaaagg acgtctgtcg ccaagccaga ttgggtgagg
    26161 gctcaggagg gcgggatgct tttggagttt tcccgccatc gacgaggcaa accgattacg
    26221 gattcgatca aacccatcgt gatgtagacg gtggccatcc aaaccaacac gaaggcccca
    26281 cccaattgaa cgcc
//
LOCUS       DAOSOR010000008        26015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_1884_length_26015_cov_4.853197, whole genome shotgun sequence.
ACCESSION   DAOSOR010000008 DAOSOR010000000
VERSION     DAOSOR010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 26015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26015
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1884_length_26015_cov_4.853197"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PK710_00875"
     CDS             complement(<1..816)
                     /locus_tag="PK710_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT08284.1"
                     /translation="MRRESITQDIVKVGKDPKSHLRIDDDLASRMHAVIEVASTDDIT
                     LIDLGNEPGTVVNGAQVNKCKIRPGDQIQVGGTLIVLESAQDAVAEPAFVPPPAPVVA
                     APPPPADAFAASPGPVDPFAAPAPAPAPAPARPVAPPVSNPFADGAGANPFGGASADP
                     FAFASPNPFAAAADPFGGGDVGAEGEGGVRYVLTKSGPDVNPDEVEVNVPAVEVMVMW
                     GDVVLHVSHLSPPRSFYVGEEQGKNLVNDYFIPAEKLGATRVPVVVVSGGSVAA"
     gene            complement(950..1219)
                     /locus_tag="PK710_00880"
     CDS             complement(950..1219)
                     /locus_tag="PK710_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08285.1"
                     /translation="MRRTKLVAAIVGLGVFLIASAAWAQRSVEGVKEGKESGYGYEFD
                     DDPLAAGGFGPNDATIRVRPGPVRTTLIRPRISFVDNMLKSVENL"
     gene            complement(1409..2869)
                     /locus_tag="PK710_00885"
     CDS             complement(1409..2869)
                     /locus_tag="PK710_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08286.1"
                     /translation="MKTHRLIGLAAVIAFAAACGGKSTTPAQTPSGPQGMHGPVVQVA
                     AKKFDEALNEMVRRDEKADWSDASCTSVAQQFLDAVKEQKDATGKGLPEASYNAGLAY
                     QRCAKHDEAKAQFQAALSANSAFHRAKVQLALYEYREKGDSVLDPTISKLTDAVVEAK
                     FQNVDALVNLAMLQMKRNGPTGASGCKDDMDCAKLNVQRALAIDDSYMPAFNQLALYY
                     MNQARARAQRETGSKRRRLMAADAEKAELSGQMLDLAALVCSQAIRKNSKYGPIYNTL
                     GLIQVEQRNINNAVQAFNTARTLDPNFFEAQMNYAAVNLSFRGFDKAEEAYRAALKMQ
                     PNNYEGRLGLSLAIRGQINDSNLDAKMAEADAELQRAKQIDASRPEAYFNEAILIQEF
                     KAKYAPDEEKKISLYENAARVFDSFIAKAGSAPEYAAAVKNANERKEDMKKIIEFIRE
                     GQKAEAEAKKAEAEAKKNPPPSPPPAAPPPPPAGAK"
     gene            complement(2899..6654)
                     /locus_tag="PK710_00890"
     CDS             complement(2899..6654)
                     /locus_tag="PK710_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08287.1"
                     /translation="MKPLRNIRALCMSAIALTWAAGVYAADAPKSGGQACWSNEAEKN
                     LASCPGSGPTTFNVGTHGKQPRVRFKSAPEQVDLKKRDAKSKPGGPTAEMDTFAIRDQ
                     RQEALKPRVRALLITEISGLEQLFQTTPRRAPDRVRIARRLAETYVELENAARRDKTQ
                     AEVKKNSGEAAKANQVMLLARKKAIHFYNLIKTQYPSDKNLDEVLYYLAYEYEQAEDL
                     VNARKVYYELIQKTPNSKYIPSAYLAFGELFFGEAQSDASKFELAKQAYTEVIKYPPP
                     DNKMFGYAHYKLAYVNWNQEDFPRSLTHFKKTIEFAEQYPNLPNAGPLAVAARKDLVP
                     VYALSGRPEAAYGFFKGLSGDKPNEESKTFDMMDQLGQNYLDTGYYAEAIALYQDLMK
                     RDRGPRYCQYHARIAEATLAMKSGNKDQIKKVLDGQVGVYKEFKSKGRTGDTQLKCAN
                     ATAGLLSETAMAWHLEAVGSGGVRGTNDPKTMALADYLYGQVVENFTAQDFAKFEFPR
                     IAKEDWPTIYKIKYHRADLLYAQKKWDQCGPAFDAVVAENPTSAEAPEAAFAAVLCYQ
                     NIYDEEHKGGKDRKGSGLMPGAKKEEKASDADKYKKKEFSERQKGMITAFNRYVCYIK
                     PADSDKEGKNQYVEVKYARARTYFEAQHWEEAALAFRDVAMNHADHEAGIFAAQLYLE
                     SINVLGDKVDPPRPDCFDKMGEDVPKLIGLYCTGARAAANEDQCTVLTRIQCDIERLK
                     AENMVKLADKGGAGSLRQYEAAANAYLGLWRKYGEEAVKSNKEMQCERLDEVVYNACR
                     AFQAGRLIAKAIQCRQILLNPDNKMTESPLARKAVYEIGGNYQAIAVYDKAADWYEKF
                     AREKPKPTAKESLDFWNEKADAALSDSVVLRLGLGHDTKALENAKLFQQYFGARKPVQ
                     NAQIAFAIANYYVEQKKWEDARGQLTKNIALIDKHAAYDVRVQAHAMLGNIYVQMKRE
                     ASAAPEYKKVMDLWANPKQAADSVLNDAKDEPEAGRTRRLGKALTAVGEAYFFFAEEK
                     RRKTVDPIKFPEYSSGAYKPSTKKPELMDSWEFEEEMAKRKKESENVKKHIDTKVKDW
                     VDKKKAAIEAADQEYQKILTLQPTPPPQWVIAAGAAVGTMWAEFVADFRRAPIPAWMK
                     SDDELRGVYYQSLDAASEPIKQRAKGAFTTCLGLSVKHQYFDRYSRSCEEWLAENYKA
                     EFHKVDEFRGSPDLVGSGLNDKPYPLQLGGEPYVVMPAATKEEEAKK"
     gene            complement(6660..8354)
                     /locus_tag="PK710_00895"
     CDS             complement(6660..8354)
                     /locus_tag="PK710_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT08288.1"
                     /translation="MLKRFACICLVVSTMGFATGSAVAQGAKGGGKAPAESAVDIELD
                     DPGSEPTGPVVAGQMTEEAAQAKRLFDSEKWAQAALALDRVFKNETNDDEGNRQIAQY
                     NLAIALYQLKLHQASYNIFSLIADNPNHLKFNETLLWLSKLATQLPEPADIIERVGKY
                     KADAIARFDNPNQHELFWQLNYLLGRYKYRNRQYEEAIRLFQNVSKRSKYFVQAQFFM
                     GISYVQLRRSVPAVKSFNRIVRAIDDGVEGVEDEQRMRDLAFLSMARTYYTASIRLDD
                     NNTPTVDATKLSAAVKYWNKIDVASEYWLDGLFEQSWAYFMAGDYPRALGNIHTIQSP
                     FFPNSFYPESDILKAVIYFANCQYKEATTVVARFKLKYEPIKAEMEKILARYKGENQE
                     AKMFDFLRNVREGKADLDAKVRPIVENSLSDRQLLRNLEYVRVLEEEERVFKKAPAAF
                     KNSPLGGDVQDALDFAKDLAIRNAGDLARARFQRTVDELNEHLRDSAKILIDVTAAER
                     NKLEQAVQSGQVSREESKIYGVVKPDEEHVLWPFNGEFWRDELGFYRQVVISNCGR"
     gene            8777..9376
                     /locus_tag="PK710_00900"
     CDS             8777..9376
                     /locus_tag="PK710_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08289.1"
                     /translation="MMRQTLVSASMVLLFGAVALACKAGGVGDPCIPNDEFSASYSGA
                     SEKGAQIEDRSFQCETRVCLVKHFRGRVSCPFGNEEGRSTPLLAEAYSGPDAKDCMVP
                     GTDKKVEVPVNSQCKARKGHVYCSCRCDGEDSAAKYCECPEGYECKEVTRSLDPRLIA
                     PGDKYCVKKEDKETDGYTCDLGCHEAPGGCGFTKNAYEF"
     gene            9388..9768
                     /locus_tag="PK710_00905"
     CDS             9388..9768
                     /locus_tag="PK710_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOT08290.1"
                     /translation="MGSIPTRRISSIGRRAEQAVEQYLLQRGYRILDRNLRLGHLELD
                     IVAQKGSLIAIVEVRHRGPTSFQSAFESIDPPKQQRLFRAAEQLWEQRFATDPSIERI
                     RLDIASVSFGPHLETVDYIEGGLD"
     gene            complement(9775..10560)
                     /locus_tag="PK710_00910"
     CDS             complement(9775..10560)
                     /locus_tag="PK710_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOT08291.1"
                     /translation="MTGTPNPSVSPPPYKRRARNYLLDPRFQLKYTGLLVLVALFLSG
                     VLGTQLWFTHKAVISQSQHAVEQGRETVRRGQKTVEESKKVSEVVAMNIAREYADNPE
                     LAKLFNADTDKHNKELAAEQMRLQEDAKALESQAAYLAVQQQKTLWVLVGGLAILVVA
                     IALAGIVFTHKIAGPVFKMSGLLRKVGQGQLRIDSGLRKGDELVEFFETFRKMVDRLR
                     ERHIDTMERLEQAIQSLEQSDVAADKLAPLKALHDEMASRLDS"
     gene            complement(10667..11500)
                     /locus_tag="PK710_00915"
     CDS             complement(10667..11500)
                     /locus_tag="PK710_00915"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT08292.1"
                     /translation="MARIRFPSKRLVAILPFLVMVTTAPAAFAGPTVAELAERLRNAT
                     DFRVRVQAALALGASKADDAVAPLCAGLDDGNATVRSAAAAGLGRLRKTGGVACLERR
                     AQSESNASVKSQIARSITQIKGGATELPSRPPDASTKWYVAVAETKNKGTRSSSEIDK
                     IVQSIVRKALLANPAAAVAPSGETAAQAQAVIQKHKVQGYLLQPTVEAPEYADSSLTI
                     RVRLTMFTYPNQALQGEFAPKLTQSGTPTEDRESENELIKMACERAVARFITVAETTK
                     P"
     gene            11726..13351
                     /locus_tag="PK710_00920"
     CDS             11726..13351
                     /locus_tag="PK710_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08293.1"
                     /translation="MVAAPRVLVRGASRLEAVASGPQDRIQIHGTLRDEVGEPIADAL
                     LLASAMAEPGVTIAWRTVGACTDESTSRSDSTDVPVTTDASGAFCFVGSLARRQAMVR
                     VVFAGSAHFNETQTIAAWNASQQSLSIAFTPRPEKLDLDADRILVFARVTPATTTSVR
                     GLPIALKTEDGSTLATEKTDDSGLVHFDVASAAMAPPGIGALTIAFAGSDHFTPAQAT
                     TSVTRSVRVALNTDTQIIRGDPSRGLALQVHALSSRGPVPSGTIEANLNGQVIGTGTV
                     MNGAADVSLTFSPSHGETRYSVDLHYRPDSPFYEPARATPVQVMAEAPSPWIRFIPVA
                     AAIIVTAWLARGWRRPPRREMRASRTPIWKGIASVDVVSASSVRNQWTGRVVDAHDGL
                     PLFGARVRVIAPTFVDEDVVMEVYADDSGHFEFRLPSVEPALRLYVETATHARVDQPL
                     PPASEMVIALASRRRLLIDRLVHWARDRGKPWYREPEPTPGQIAKVARAHHGNEVAVW
                     AEQVERRAFGSDLVDQRIEKEVHDLEPRGHALR"
     gene            13420..14859
                     /gene="ftsY"
                     /locus_tag="PK710_00925"
     CDS             13420..14859
                     /gene="ftsY"
                     /locus_tag="PK710_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOT08294.1"
                     /translation="MEIVGVIIAIVVIVAIYWFVIRKPGSVEQLPEPQEPKRIAAPPD
                     RPKAGKQTGQEPAKAKAQTASQKAESESSPSVPKKTESKAPEAKKPETQPTDSSVRSS
                     PQTAQAAQESPAAETEPAEPKTTGTESSEDAEPVQQPPSAKEPAEDSPAPLVAQAVDV
                     QVTTRARDVQGLRKGLGKSRTSDGFFGRLAKLITGRKEIDPSIVDEIEEVLITSDVGV
                     KTTEAILERLREGLRSKEFNDSDKVWDALRREAVRILSLGAGGLTLRSKPTVVLMVGV
                     NGSGKTTTIGKLATQLKADGSKVLLAAGDTFRAAAVQQLQVWGDRVGVDVVKGKDGAD
                     AGAVIFDAIRRGHEQQVDVVLADTAGRLHTKTNLMEELKKVVRTMGKALEGAPHEILL
                     VLDATNGQNAVAQSRQFAEALPLSGIILTKLDGTAKGGVILGICDELKVPVRYVGLGE
                     KADDLRPFDAEEFVEALLGGAESEDNEAA"
     gene            14941..15990
                     /locus_tag="PK710_00930"
     CDS             14941..15990
                     /locus_tag="PK710_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HOT08295.1"
                     /translation="MAVFYNGSNNLGLSTARRNDAEGLTMKQARTGLLLGMALLLVSA
                     IGVAQVKKATTAEEVADGDDVEFDADAPEEGEDDVETDEGGGAVGFEEGLGDICKVDP
                     DACPKISMDEASKQDLNPQIYAVQQIYALRRERFELQPYWSFTMNDQFVSHPAPGVAL
                     NYYITNVMAIGANFNWYQGLNTDSEFNAATRRAVRVGVPLNEYQWSAALNFTYVPMYG
                     KFAGFGDFIFHWDGYVVGGVGAISTRPIVVFDPRYRNFSFEPKIAFNAGIGARIFFNR
                     WFAAIIELRDYAYLEELENTANYDAMFSSDDARTAWRQDEANWTDEKKLTNNVQAQVG
                     ISIFLPFSWEYRLPK"
     gene            16045..16941
                     /locus_tag="PK710_00935"
     CDS             16045..16941
                     /locus_tag="PK710_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08296.1"
                     /translation="MRRIFATGLLAVVGVLAMESTASAQEIQLTGPLAGAPAVRELRW
                     HRNKRFEIAPAISFTLLDEYQRTIFAGARLQYHLTDWLGLGAWGAYGVVHLNTALTDE
                     IDKVNKDRWDSSRDNDALTAVDRNASLLSVGYDFPQQLGKIQWVVAPQVTAVPFRGKL
                     ALFEKAFVDTDLYFFAGPAFIGVVEREDYSVADFGNPNKTVLENGKPVKSYPTASRVA
                     IAPTFGAGLSFFPGSWSGIGLEYRALPFSWNNSGFDSQGGPPDENGPDSIVDEKDRQF
                     RFNQMITISFSVFLPTDIKVSP"
     gene            complement(17189..17932)
                     /locus_tag="PK710_00940"
     CDS             complement(17189..17932)
                     /locus_tag="PK710_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT08297.1"
                     /translation="MGLFDIFKRSKAEDSDDSKHDKNVARLGRMAANKNAQAYDRLEA
                     IQSLCTIATADSAAALLKRFSFYIEPSITDQEEKEIAFRGIIAAGPAAVEPIREFCMK
                     AESFTWPMKMLEELLDEEAFVDELLVLLERFDTDYSKNQEPKLQIIAELEGKKRDDVR
                     VAVERFLDDFDERVRFHTVATLFSQEDRTSVTALMRALISEESVRIKNRIAEGMLARE
                     WVCPAELSNDIMNALPTGYALREGIVRKN"
     gene            complement(18028..19488)
                     /locus_tag="PK710_00945"
     CDS             complement(18028..19488)
                     /locus_tag="PK710_00945"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HOT08298.1"
                     /translation="MRWKIITVNSVVIVLLGVFLSALLWTQLKSLVANEAAIVSDASQ
                     AVSAANAQLQLDALQVERWLANQVNEPALREPFELAVREARAEAATKQANGLREAAIQ
                     TPALAGLHPAVVAFVDINGLSLGRNGNNLMRGEDIGKAHPGMKEAIVHGQTGSETWFA
                     PNLSQQWLVSFAPVRNASGQVLGSVIYGTPLNDERMTRTIEKTSGGAIVVAVEGPKGI
                     EKAAKSKTVDATAANAIAEGPLAESVKGAMRFDAAEKLVGGPKDWIYVGAPLGGYGGT
                     KAVLIAVSPASVMDVGGVFYAIFGMVVLGLILVGIAGWLLGNYISKPVEELEEGLLQI
                     LNGRTDVRFEIEHAVLGGVVFRINTLLNQLMGVQEDDTDEDGRPSLAPSTNAFQGALD
                     VDAPAAAASAEKVDPAVASALAAEPAEQYYARLFQEYVNAKRSIGDAVDHITKDSFVE
                     RIRQREAEQSAKSGRPVRYQVQLRGREVNLLAVQLP"
     gene            complement(19684..19758)
                     /locus_tag="PK710_00950"
     tRNA            complement(19684..19758)
                     /locus_tag="PK710_00950"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19724..19726),aa:Gly,seq:gcc)
     gene            19946..21148
                     /locus_tag="PK710_00955"
     CDS             19946..21148
                     /locus_tag="PK710_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT08299.1"
                     /translation="MNYQQGKDVMLSAAVTSETPTNWGLLRVKGDGSLSPLPLFDGAI
                     VDVTCDESHHDGDTSHVRHHAQFIVRSEGVYVSDLDSGQGTWVDGIAVRKLGLAHGSV
                     LRCGRCIALFVERDLAQSASPPKRKGNVVIGVRLQACLDTALGFIHGKRSFLVEGNAG
                     VGKSTMVDAALRKAGETTPVCHLDGACFESEALVRAFERTSPCVWVVRHFEQFPRAAQ
                     ADLVRTVRMMDGSFLIGTVVGSVEHAHAEGRVCSSCMTLMGSHVIRVPSLIERREDIP
                     MLTMEYLRRTEVPMTFDVMGLFECVLRAGWTGGIRELQDHLSAAFSSDSVSEDPIKTL
                     ARSIPRPCSKEPLSLSSSDPDLARVRLQKALTMASGTVAEAARNLRISRQTFYRELRR
                     LSVEATRS"
     gene            complement(21149..23347)
                     /locus_tag="PK710_00960"
     CDS             complement(21149..23347)
                     /locus_tag="PK710_00960"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOT08300.1"
                     /translation="MALNLPSSRTGTLPLEVLIERVLAYQPTADTELLRKAYDFANSA
                     HEGQVRKSGDPYFAHPAAVAGVITELRLDAACVCAGLLHDVVEDTAQSVDDLEKVFGK
                     EVAFLVDGVTKLSKLEFSSRQERDAENLRKMLVAMSRDIRVLLLKLCDRVDNMRTLEF
                     MKPDSQERIARETREVFAPLAARLGIHRFKTELEDLSFQFLETEAHQNLSRLMGETKA
                     VRERFSREICEELRKQLHDHGFAGEVYRRTKHLYSIHRQMVEYNLDFEQLRDLVAFHV
                     IMESVADCYGVMGAIHSRYTPIPGRIKDYIALPKPNMYQSLHTTVIGTRRERVEIQIR
                     TKEMDRVAQEGIAAHWIYEDRLSGGADPKLMQRLGWLHDLLDVQREVKDAAEFLDSIR
                     IDLFQDEVYAFTPKGEARVFPKGATAIDFAYAIHTEIGHHSSSARVNGTVSSLRHKLR
                     NGDMIEMITSPDQRPKKEWLEFAATSRARNRIRAYLRNEERERSSKLGKELLEKELHD
                     NGFSLQRFTKDDAELGRVLGELEIPTLQDLFIAVGYGKVAPASVTQTLSRSVDDGAKL
                     KPKASVREGRIEHFVRRLMGKDVQGLKVSEIDDVLVRFARCCNPLPGDEIIGFITRGR
                     GITVHRRDCTKAFETDPDRRIAVVWDSRVKANRSVQLKVLTVNRPGLLASMGQAFSDQ
                     DINLTAVTSRAVEDGRAMNIFTFPCADLSQLKIIMRALRKVQGVFSVERV"
     gene            23555..24112
                     /gene="frr"
                     /locus_tag="PK710_00965"
     CDS             23555..24112
                     /gene="frr"
                     /locus_tag="PK710_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOT08301.1"
                     /translation="MIDEIIGDLNNSIGKSQEAFKRDLSKLRTGRAHAGMLDSIRVDY
                     YGTATPLNQLATISVPEPRLLTVRPWDKGAVKAVDKALRESDLGINPLLDGDVIRIPI
                     PQLTEERRKELVKLVKRMGEDCKVAVRKHRHDAMDLLSSLQDEGEASEDEVGRARKRV
                     EEVVAEAVRKIDEIQQAKEKDVFEN"
     gene            24193..25032
                     /locus_tag="PK710_00970"
     CDS             24193..25032
                     /locus_tag="PK710_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOT08302.1"
                     /translation="MGKPNPPHRMRIVASGLTDVGLQREHNEDSFLVLEEHDLFIVAD
                     GMGGHRAGDVASRVATETMAEFFRSTANDDVTWPYHFDTSLSEEENRLLTGIRLANRQ
                     IFERSIRSREYHGMGTTMVSALFARHSGKLYIGHVGDSRCYRLREGEVRLMTRDHSLV
                     NDYLLAMPELSEDQKGELPKNVITRALGMQDHVVVDLQCDEPIEGDIYVLCSDGLSGM
                     VPDESILETLSDSTDPEQICRRLIETANHNGGEDNITAVVMRLVADDRPDDEPESEPL
                     DDL"
     gene            complement(25061..25615)
                     /locus_tag="PK710_00975"
     CDS             complement(25061..25615)
                     /locus_tag="PK710_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08303.1"
                     /translation="MSVSRYLGSGFALISVLGLGGCSDPSSPVPQGAFTVTFGNPVGS
                     SVTCPAMQPSLLQVGAVGPAQHATISDGDDDATIECSVVKKGQGFVASGSISKGLTRF
                     RLVPTAVGEGNSNQGRIFVAGANTAGKSYGASAESPCNFQLIEGEEGKIWLSFECPLI
                     ETGSSENEKCSLVNGYLFFENCES"
     gene            complement(25728..25804)
                     /locus_tag="PK710_00980"
     tRNA            complement(25728..25804)
                     /locus_tag="PK710_00980"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25768..25770),aa:His,seq:gtg)
BASE COUNT         5533 a         7402 c         7137 g         5943 t
ORIGIN      
        1 cgccgccaca ctgcccccac tcaccacaac caccggaact cgcgtcgctc ccagtttttc
       61 ggcaggaatg aagtagtcat tgacaagatt cttgccctgc tcttcgccca cataaaagct
      121 tcgaggcggg gataagtgag agacgtgcaa cacaacgtcc ccccacatca ccatcacctc
      181 caccgccgga acgttcacct ctacctcgtc cgggttcacg tccggcccgc tcttcgtcaa
      241 cacatagcga actccccctt ccccttccgc accaacatcc cctcccccaa acggatccgc
      301 cgccgcggca aatggattcg gcgaagcaaa cgcaaacgga tccgcactcg ctcctccgaa
      361 aggattcgca cccgctccgt cggcaaaagg attcgatacc ggcggcgcca ccggtcgcgc
      421 gggagccggt gcaggagccg gcgcaggggc agcaaaagga tccaccggtc caggcgaagc
      481 ggcaaacgca tcggccggag gcggaggcgc tgccaccacc ggcgcgggcg gcggcacaaa
      541 cgcgggctcc gctaccgcat cttgcgccga ctccaacacg atcaacgtgc cacccacctg
      601 aatctgatcg cccggtcgta tcttgcactt gttgacctgc gcaccattca ccactgtccc
      661 aggctcgttt cccaagtcaa tgagcgtaat gtcatccgtc gacgctacct caatcactgc
      721 atgcatccgc gaagcaagat cgtcgtcgat ccgcaaatga cttttcggat ccttgccgac
      781 cttgacaatg tcctgcgtga tcgattcacg acgcactagt tggtcgcctt ggtacaatgc
      841 gaacgttaga gtaaccttca tccctgcctg acctttcacc ggacgacaac ctcagcgcca
      901 gccgcccgta agctgacacc cgaaactcct cgtttgcgcc tgtcgcccgc tacaagtttt
      961 cgactgactt caacatattg tcgacgaagg agatgcgcgg acgaatcagt gtggttcgga
     1021 ccggcccagg gcgaactcgg atggttgcgt cgttcggccc aaatcccccc gctgcgagag
     1081 gatcatcgtc gaactcataa ccgtatccgg attctttacc ttctttcact ccttccaccg
     1141 aacgctgtgc ccacgcggca gaagcgatga gaaaaacccc caggcctaca atggctgcca
     1201 ccaacttcgt tcgcctcatc gttgactcca taggatttga tcgttttttg ttccaataaa
     1261 agtgtaatca ggttcgagcc ttacggtcaa tgatcaatct tgaggtgcgc tcgaatcggt
     1321 ggaccatgag accaccgaca acccgcccga acacaagttc ccccacgaga taaaaatgtt
     1381 cggtcggttc gaccaaatag tctttggttt acttggcacc ggcgggcggg ggcggaggag
     1441 cagcaggagg aggcgacggc ggagggtttt tcttcgcttc tgcttcggcc tttttggcct
     1501 ccgcctcggc cttttgacct tctcgaatga actcaatgat ttttttcatg tcttcttttc
     1561 gttcattcgc gttttttacg gccgctgcat attccggagc cgagccggct ttcgcaatga
     1621 acgaatcgaa cacccgcgcg gcgttctcat aaaggctaat ctttttttcc tcgtcaggag
     1681 catatttcgc cttgaattcc tgaatcagga tcgcctcgtt gaagtacgcc tccggccgcg
     1741 acgcatcaat ctgcttggct cgctgcaact ccgcatccgc ttccgccatc ttggcgtcca
     1801 agttgctgtc gttgatttga ccacgaatcg ccagcgagag ccccaaccgc ccttcatagt
     1861 tgttcggctg catcttcaac gccgcacgat acgcttcttc cgccttgtca aacccacgga
     1921 acgacaagtt gaccgccgca tagttcatct gcgcttcaaa aaagttcggg tccaacgtcc
     1981 gcgccgtgtt gaatgcctgc accgcgttgt tgatattgcg ctgctcgact tgaatcagac
     2041 ctaatgtatt gtaaataggc ccgtattttg agtttttccg aatggcctga gaacagacga
     2101 gcgcagccaa atcaagcatc tggccgctca gttccgcctt ctccgcatcc gccgccatca
     2161 atcgtcgacg cttgctgcca gtttcccgct gagcacgagc acgcgcctgg ttcatgtaat
     2221 acaaggcgag ctgattgaac gccggcatgt agctgtcatc gatcgccagc gctcgctgca
     2281 cgttgagttt cgcgcagtcc atatcgtctt tgcagcccga tgcccccgtc ggtccgtttc
     2341 gcttcatttg aagcatcgca aggttcacca aagcatcgac gttttgaaac ttggcttcca
     2401 ccacggcatc cgtgagtttc gaaatggtcg gatccaaaac cgaatcgccc ttttcccgat
     2461 actcgtatag ggcgagctgc actttggccc ggtggaacgc ggaattcgcg gataatgcgg
     2521 cttgaaactg cgccttcgct tcgtcatgtt tcgcgcagcg ttggtacgct aggcccgcat
     2581 tgtacgatgc ctccggcaac cctttgcccg tagcatcttt ctgctccttg accgcgtcca
     2641 aaaactgctg cgccacactc gtacacgagg cgtcgctcca atccgctttt tcatctcgac
     2701 gaaccatctc attgagcgct tcgtcgaatt tttttgccgc tacctgcaca acagggccgt
     2761 gcatcccttg cggccccgaa ggcgtttggg cgggagtcgt gcttttgcca ccacaagccg
     2821 ctgcgaacgc gatgaccgca gcaagcccga tcagtctatg cgttttcatc ggtgctctcc
     2881 tccgttgggc gttcgcgatc acttctttgc ctcttcttcc ttcgttgccg ctggcatgac
     2941 cacatacggc tcaccgccaa gctgcaacgg ataaggctta tcattcaatc cggaacccac
     3001 gaggtccgga ctcccgcgaa actcgtccac cttgtggaat tccgccttgt agttctccgc
     3061 caaccactcc tcacaggacc gagaataacg gtcgaagtat tggtgcttga ccgatagccc
     3121 taggcacgtc gtaaacgccc ccttcgcacg ctgtttgata ggctcgctcg ccgcatcgag
     3181 actctgatag tacacgcccc gcaactcatc gtcgctcttc atccacgcgg gaatcggcgc
     3241 acgacggaag tccgcgacga actcggccca catcgttccg actgcagcac ccgcggcgat
     3301 gacccactgg ggaggtggcg taggctgaag agtaaggatt ttttgatact cttggtccgc
     3361 agcctcgatg gccgccttct tcttgtcgac ccagtccttg accttagtat cgatatgctt
     3421 cttgacgttt tcagattcct tcttgcgctt cgccatctcc tcttcgaact cccacgagtc
     3481 catgagttca ggcttcttgg tagagggctt gtaggcaccg ctcgagtact cagggaactt
     3541 gatcgggtcc accgtcttgc gacgtttctc ctccgcaaag aagaagtacg cttcaccgac
     3601 ggctgtcagg gctttgccaa ggcgacgagt tcgtcccgcc tcgggttcgt ctttcgcatc
     3661 gttgaggacc gaatcggccg cctgtttggg gtttgcccat aggtccatga cttttttgta
     3721 ttccggagcg gcactggcct cacgtttcat ctgcacatag atgttgccaa gcatcgcgtg
     3781 cgcttgcaca cgcacgtcgt aggcggcgtg cttgtcgatg agggcgatgt ttttggtgag
     3841 ttgcccccgg gcatcctccc actttttctg ttccacgtag taattggcga tcgcgaacgc
     3901 tatctgtgcg ttttgaaccg gttttcttgc gccgaagtac tgctggaaca gcttcgcgtt
     3961 ctcgagggct ttggtgtcat gtccaagtcc caagcgaagc acgaccgagt cggacaaggc
     4021 ggcgtcggcc ttttcattcc aaaaatcgag tgattctttc gcggtcggct tcggcttttc
     4081 tcgcgcgaac ttctcgtacc aatccgcagc tttgtcatag accgcgatgg cctggtagtt
     4141 gcctccgatt tcatacacgg ccttccgagc caacgggctt tccgtcatct tgttgtcagg
     4201 attgagcagg atctgacggc actgaatcgc tttggcaatc aatcgacctg cttggaacgc
     4261 cctgcaagcg ttgtacacca cctcatcgag ccgctcgcat tgcatttcct tgtttgattt
     4321 gaccgcttct tcgccgtact tgcgccacag gccaagataa gcgttggcag ccgcttcgta
     4381 ttgacgaagc gaaccggcgc cacccttgtc cgcgagcttg accatgttct cagccttgag
     4441 acgttcgatg tcgcattgaa tgcgggtgag tactgtgcat tgatcctcat tggcggccgc
     4501 tcgcgcaccc gtgcaataca gcccaatgag tttgggaaca tcctcaccca tcttgtcgaa
     4561 gcaatcagga cgcggcgggt cgactttgtc gcccaacacg ttgatggatt cgagataaag
     4621 ctgggccgcg aaaatgccag cctcgtggtc cgcgtggttc atcgccacgt ctcggaacgc
     4681 aagagccgcc tcctcccaat gctgcgcttc gaagtaagtc cgcgcgcgcg cgtatttgac
     4741 ctcgacgtat tgattcttgc cttccttgtc ggaatccgcg ggcttgatgt agcaaacgta
     4801 gcggttgaaa gccgtaatca tgcccttctg ccgctcggag aactccttct tcttgtactt
     4861 atccgcatca gaggctttct cttccttctt ggcaccaggc atcagaccgc tgcccttgcg
     4921 atccttgccc cccttatgct cttcgtcgta gatgttttgg tagcaaagca ccgcggcgaa
     4981 cgctgcttcc ggagcttccg cgctcgttgg attttccgct acgacagcgt cgaaagcggg
     5041 gccacattga tcccatttct tctgggcgta gagcaagtcc gcccggtgat atttgatttt
     5101 gtaaatcgtc ggccaatcct cttttgcgat gcgcgggaat tcgaacttcg caaagtcttg
     5161 cgccgtgaag ttttcaacga cttgcccgta gagataatcg gcgagagcca tggtcttggg
     5221 atcgttggtt cctcgaaccc ctcccgagcc gaccgcctcg agatgccacg ccatcgccgt
     5281 ctcactcaga agcccagccg tggcattggc acacttgagt tgcgtgtccc ccgtgcgccc
     5341 cttggacttg aactctttgt atacgcccac ctgaccgtcg agcacctttt tgatctggtc
     5401 cttgtttcca gacttcatcg ccagcgtggc ctcagcgatg cgcgcgtgat actggcaata
     5461 tcgcggcccc cgatcacgct tcatcaaatc ctgatacaag gcgatcgcct cggcataata
     5521 gccagtgtcc agatagttct gcccaagctg gtccatcata tcgaaggtct tgctttcctc
     5581 attcggtttg tcgcccgaca accccttgaa gaagccatac gccgcctccg gccgaccgga
     5641 taacgcgtac acgggaacca agtcttttcg ggctgccacc gccaacggac cagcgttcgg
     5701 caggttgggg tactgctcgg caaactcgat cgtctttttg aagtgcgtca acgaacgcgg
     5761 aaaatcttcc tggttccagt tgacataggc gagcttgtaa tgggcgtagc cgaacatttt
     5821 gttgtccggc ggcggatact tgatgacctc ggtatacgcc tgtttggcta gctcgaattt
     5881 ggaagcgtcg gactgagcct ccccaaagaa caattcgccg aacgccaggt acgcgctcgg
     5941 gatgtacttt gaattcggag ttttttggat caactcgtaa tagaccttgc gagcgttgac
     6001 caagtcctcg gcctgctcat attcgtacgc caaatagtac aacacctcgt caaggttctt
     6061 atccgatggg tactgcgtct tgatgaggtt gtagaagtgg atggccttct ttctggcaag
     6121 caacatgact tgattggcct tcgcggcttc cccggagttt ttcttgacct ccgcttgggt
     6181 tttgtcgcga cgtgccgcgt tctcgagttc cacgtacgtt tccgcgagtc gtcttgcaat
     6241 acggacgcga tcgggggcgc gacgcggggt ggtttggaac aattgctcga gcccgctgat
     6301 ttccgtgatg agcagcgccc ggacacgagg cttcagcgct tcttgtcgtt gatcgcgaat
     6361 ggcaaacgta tccatttctg ctgtcggccc gcccggtttg cttttggcat cgcgtttttt
     6421 caggtcgacc tgttcaggag cgctcttgaa ccgaacacgc ggctgcttgc cgtgggtccc
     6481 cacgttgaac gtggtcggcc cagaccccgg acacgaggct aagttctttt cagcctcgtt
     6541 cgaccagcag gcctgtcctc ccgacttggg ggcgtcggcg gcgtacacgc ctgcagccca
     6601 tgtgagcgcg atggcgctca tgcacaacgc gcggatgttc cgcaatggct tcatgatgcc
     6661 tacctcccgc agttcgaaat cacgacctgc cgataaaatc ccagttcgtc gcgccagaac
     6721 tcaccgttga acggccaaag cacgtgctct tcatccggct tgaccacacc atagatcttt
     6781 gattcttcgc gtgaaacctg tccgctctgg acggcttgct cgagtttgtt tcgttcggcc
     6841 gcggtgacgt cgatcagaat tttggccgaa tcacgaagat gctcgttgag ttcgtcgaca
     6901 gtacgctgga accgggccct ggccaggtca ccggcattgc gaatcgcaag gtctttcgcg
     6961 aagtcgagcg catcctgcac atcgccccct agaggcgagt ttttgaaagc cgcgggcgct
     7021 tttttgaata cccgctcttc ttcttcgagc acccgaacat actccaagtt gcgcaacaac
     7081 tgcctgtcgg acaacgagtt ttccacgatg ggccggacct tggcatcgag gtccgccttg
     7141 ccctcacgta cgtttcgcag aaaatcgaac atcttcgcct cttggttctc acccttgtag
     7201 cgagcgagta ttttctccat ttcggctttg atcggctcgt acttcaactt gaaccgagcg
     7261 accacggtgg tggcctcctt gtattgacag ttcgcgaaat aaatgaccgc tttcaaaata
     7321 tccgattccg gatagaagga gttcgggaaa aacggtgact gaatcgtgtg gatgttgcca
     7381 agagcacgcg gatagtcgcc cgccatgaag tacgcccacg actgctcgaa caaaccgtcg
     7441 agccagtact cgctcgccac gtcgatcttg ttccaatatt tgaccgctgc actcaatttc
     7501 gtggcatcca cggtgggcgt gttgttgtca tccaaccgga tcgaagccgt gtaataggtg
     7561 cgggccatcg acaagaacgc gaggtcacgc attcgctgct cgtcttccac tccctccaca
     7621 ccatcatcaa tcgccctcac gatgcgattg aaggatttga cggctggaac cgagcgacgg
     7681 agctggacgt aagaaattcc cataaagaac tgggcttgca cgaagtactt gcttcgcttg
     7741 cttacgtttt ggaaaagccg gatggcttcc tcgtattgcc ggtttcgata cttatatcgt
     7801 ccgagcaagt aattgagttg ccagaacagt tcgtgctggt tggggttatc gaaacgagcg
     7861 atggcatccg ccttgtactt gcccacccgt tcgatgatgt cggcgggctc gggcaactgc
     7921 gtggcgagtt tggaaagcca caacaaagtc tcgttgaact tcaggtggtt ggggttgtcc
     7981 gcgatcaagc tgaaaatgtt gtaactcgcc tgatgcaatt tgagctggta cagcgcgatc
     8041 gcgaggttgt attgcgcgat ctgtcggttg ccctcgtcat cattcgtctc gtttttgaac
     8101 actcgatcga gagccaaagc cgcctgggcc catttctcgc tatcaaataa gcgcttggct
     8161 tgagcagcct cctccgtcat ctgcccggct acgaccgggc ctgtaggctc acttcccgga
     8221 tcgtcgagtt caatatcgac cgccgattcc gcgggggctt ttcccccgcc ctttgcgcct
     8281 tgggctacgg cgcttccggt tgcaaagccc atggtactga cgaccaggca gatgcaggca
     8341 aaccgcttta gcatcgcttt tcctcgcttt cggcgacgtt tctctctgag tccgtctggg
     8401 tgttctttcg gaccccgaaa aaggtaaaat aatcaatgat gacttggaga tggctcccgc
     8461 aaggggcgtc atgttaacga ttcgtcggaa aggttgtcaa gcgcctcctc cccccccttg
     8521 tcggctcgat gtcgcttcgt cgtcgtcgcg ccgtccgttc cgccattcct ttgcgtagag
     8581 aaactcgctt atcgccagcc gcatccaaaa ccgatattca ccacagcgcc agttccccgt
     8641 tgactccgcg ggcggctatg atctagtttg cgttgtcact gtttaggcgt acggtggaca
     8701 attacggttg cctatcgtcg aacgtataac tatttcgagc tgttgcccga aatccgtcct
     8761 cagggagccc accacgatga tgcggcagac actcgtgtcc gcttcgatgg ttttactgtt
     8821 cggagccgtc gccctcgctt gtaaagctgg cggtgtcgga gacccctgta ttccgaacga
     8881 tgaattcagc gcatcttact ccggtgcttc ggagaaaggc gcgcagatcg aagaccgttc
     8941 ctttcaatgc gaaacgcgcg tctgtctcgt gaagcatttc cgaggtcgcg tgtcctgtcc
     9001 tttcggaaac gaagaggggc gcagtacgcc tctcttagca gaagcttact cgggaccaga
     9061 tgccaaagac tgcatggtgc cgggaaccga taagaaggtc gaggtccccg tcaactccca
     9121 gtgcaaagct agaaaaggcc acgtgtactg ctcgtgccgc tgcgatgggg aggactcggc
     9181 agccaaatac tgcgagtgcc ccgaaggcta cgaatgcaaa gaggtgactc gttccctcga
     9241 cccacgactc atcgcccccg gtgataagta ttgcgttaaa aaagaagaca aagagaccga
     9301 tggatatacc tgtgacctcg gttgccacga agcacccggc ggctgcgggt tcaccaaaaa
     9361 cgcctacgag ttctaatccc tccgcccatg ggttccatac ccactcgtcg aatctcttcg
     9421 ataggtcgac gcgccgaaca agctgtcgag cagtatctcc tacagcgcgg ctaccgcatt
     9481 ctcgaccgaa atcttcgact cggacacctc gaactcgata ttgtcgccca aaaaggctcc
     9541 ctcatcgcca tcgtcgaagt gcggcatcgc gggcccacct cttttcaatc cgccttcgaa
     9601 agcatcgacc ctccgaaaca acagcgtctc tttcgtgccg ccgaacagtt gtgggaacag
     9661 cgattcgcca cagacccatc catcgaacgc atccgcttgg acatcgcaag tgtatcgttc
     9721 ggcccccatt tggaaaccgt ggattatatc gaaggcgggc tggattgatt ccgctcagct
     9781 atcgagacgc gaagccatct cgtcgtgaag cgctttgagg ggggcaagtt tgtccgccgc
     9841 cacatcgctc tgttccaagg attgaatagc ctgctcgagc cgctccatgg tatcgatgtg
     9901 ccgctctcgc aaacggtcga ccatctttcg gaaggtctcg aaaaattcga cgagttcatc
     9961 gccctttcgg agaccggaat cgattctcag ttgcccctgg ccgactttgc gcaacaaacc
    10021 gctcatcttg aatacgggcc ccgcgatctt gtgcgtgaaa acaattccag caagcgcaat
    10081 ggccaccacc aaaatggcaa gcccccccac caacacccac agcgtttttt gttgctgaac
    10141 cgcaagatac gccgcctggg attcgagcgc cttggcgtct tcctgcaatc gcatctgttc
    10201 agcggcaagt tccttgttgt gcttgtccgt atccgcgttg aacagcttcg cgagttccgg
    10261 attgtcggcg tactctctcg cgatgttcat cgcgacgacc tcagatactt ttttactttc
    10321 ttcaacggtt ttctgtcctc gtcgaaccgt ttctcttcct tgctcgacag cgtgctggct
    10381 ttgagagatg acggctttgt gggtgaacca cagttgggtt cccagcacgc cggatagaaa
    10441 cagggccacc agcaccagca gacccgtgta cttgagctga aatctcgggt cgagcaggta
    10501 attgcgcgct cgacgtttgt agggaggagg tgagacagaa gggtttgggg ttccagtcat
    10561 gatgagcctc ttgagcagtc agacgcccga aggatcgttt tgagacacga acggtagggt
    10621 agctggtcca atgccatacg gacaacaaat cccttgttca cccattttat ggtttggttg
    10681 tctccgccac cgtgatgaac cgggcgaccg cgcgttcgca ggccatcttg atcaattcat
    10741 tttccgattc gcggtcctcg gtcggggtcc ccgattgcgt gagtttcggc gcgaattccc
    10801 cttgaagcgc ttggttagga tacgtgaaca tggtcagacg cacccgaatc gtaaggctcg
    10861 agtccgcata ttcgggagct tccacggtgg gttgaagcaa atacccttga accttatgct
    10921 tctgtatcac agcttgcgcc tgggcggccg tctctcccga aggcgccacc gcggcggccg
    10981 gattcgccaa taacgccttg cgcacgattg attgaactat tttgtcaatc tccgatgaac
    11041 tgcgcgtccc cttatttttg gtttccgcta ccgccacgta ccacttggta gaagcatccg
    11101 gtggacgcga cggcagctcc gtagctcccc ctttgatttg ggtgatggat cgcgcgattt
    11161 gagacttgac cgaagcgttc gattcggatt gggcgcgtcg ctccaaacac gccacacccc
    11221 cagtttttcg aagccgccca aggccagccg cagcagcact gcgaaccgtg gcattgccat
    11281 cgtcgagacc agcacatagc ggagccaccg cgtcatcggc tttcgacgcc cctagcgcta
    11341 gagccgcttg cacacggaca cgaaagtccg tcgcgttgcg aagccgttcc gccaattccg
    11401 cgacggtagg tcctgcaaac gccgcaggcg cggtcgtcac catgacaaga aatgggagga
    11461 tcgcaaccag tcgtttcgat ggaaagcgaa ttcgagccat acaaccaccc agattaccac
    11521 ggggcgaacg aggaaggatc ttgtatccta ttggttccgg atttcggaat cgacttcata
    11581 gatcgacgaa ccaatgaccg agcttgttca gacaaaatgt cgaatttttc tccgtcgacg
    11641 taatctcagc ccgttttcag ccctgttggc ccgttgcttc gggtctttat tgctgtcggg
    11701 ccttctcgtc ctacatccaa ttccgttggt agcggccccc cgcgtcctcg ttcgaggcgc
    11761 ctccaggctc gaggccgtcg cgtcaggtcc ccaagatcgc attcagattc acggcacgct
    11821 tcgtgacgag gttggcgaac cgattgccga cgcccttttg ctcgccagtg ccatggcgga
    11881 accgggggta accattgcct ggcgcaccgt cggcgcctgc accgatgagt ccacctcccg
    11941 ttcggactct accgatgttc ccgttacgac ggatgcatcc ggcgcatttt gtttcgtcgg
    12001 ctcgcttgcg cgaaggcaag ccatggttcg agtggtattt gccggctccg cacatttcaa
    12061 cgaaacgcaa accatcgcgg catggaatgc ctcgcaacaa tccctatcga tcgccttcac
    12121 accccgtcca gaaaaactcg atctcgatgc ggaccgcatt cttgttttcg cccgagtcac
    12181 cccagcaacg acaacctccg ttcgaggctt acccatcgcc ttgaagacgg aagatggttc
    12241 gacactggcg acagagaaga cggatgacag cggtctcgtt cactttgacg tcgcgagtgc
    12301 ggcaatggca ccacctggaa ttggtgctct gacgatcgcg tttgcgggct ccgatcattt
    12361 cacgcccgcc caagctacca cgagcgtgac acgctcggtg cgggtggcgc tcaacaccga
    12421 cacccaaatc attcgaggtg acccgtctcg agggcttgcc cttcaggtgc atgcgttatc
    12481 ttcccgcgga cctgttcctt cgggaacgat cgaggcgaac ctcaatggac aagtcattgg
    12541 caccggcacc gtgatgaacg gcgctgccga tgtttccctt accttttccc caagccatgg
    12601 tgagacacgc tattcggtgg atttacacta tcgtccggat tccccgtttt acgaacccgc
    12661 gagagcaaca cctgtccaag tcatggcgga agctcctagt ccgtggattc gattcatccc
    12721 ggtcgccgcc gccatcatcg tcacagcgtg gttagcccgt gggtggcgtc gtccaccacg
    12781 tcgtgaaatg cgcgcctcgc gtacccccat atggaaaggt atcgcctccg tcgacgtcgt
    12841 atccgcatcg tcggtgcgca accagtggac cggtcgtgtc gtggatgcgc acgatggttt
    12901 gccgcttttt ggcgcgcggg tacgagtgat cgcgcccacg ttcgtcgacg aagacgtcgt
    12961 gatggaggtg tatgccgacg acagcggtca tttcgaattt cgactgccat cggtggagcc
    13021 ggctcttcgg ctttatgtgg agacagcgac ccacgcgcgg gtcgaccaac cccttccccc
    13081 agcaagcgag atggtcatag ccctggcaag tcgtcgaagg ttattgattg accggttggt
    13141 ccactgggct cgtgaccggg gaaagccatg gtatcgtgag cctgaaccga cacctgggca
    13201 gattgccaag gtcgctcgcg cacatcacgg gaacgaagtt gcagtatggg cggagcaagt
    13261 cgagcgaaga gccttcgggt cggacctcgt ggatcagcgt atagaaaagg aagttcatga
    13321 cctcgaaccg cgaggacacg ctctacgttg acgcgctggg atccctcgtt ttatggtgcg
    13381 ctaccatcgc acgctacgtg cagcgggaga aattgactca tggagatcgt tggcgtcatc
    13441 atcgcgatcg ttgttatcgt cgcgatttat tggtttgtca ttcgcaagcc tggctctgtc
    13501 gagcaacttc ccgaacccca ggagcccaag cgaatcgctg ctccaccgga tcgtccgaag
    13561 gcaggcaagc agaccggtca agaaccggcg aaagcaaagg cacagacggc ttcccagaaa
    13621 gcggaaagcg aatcctcccc atcggttcca aagaaaactg aatcgaaggc tcccgaagca
    13681 aagaaaccgg aaacgcaacc aacggattct tccgttcgat cttccccgca aacggcgcaa
    13741 gctgcacaag agtcgccagc ggcagagact gaacccgcgg aaccaaagac gacgggaact
    13801 gaatcgtcgg aagatgccga gcccgtgcag cagccaccgt cggcgaaaga gcccgcggag
    13861 gactcaccag cgccacttgt tgcgcaggca gtggacgttc aggtcacgac acgtgcgcgc
    13921 gacgtgcaag gacttcgaaa ggggttgggc aagtcaagaa cgtcagatgg attttttggt
    13981 cgtcttgcga agttgatcac gggtcgaaaa gaaatcgatc cgagtatcgt ggacgaaatc
    14041 gaagaggtat tgatcacgtc agacgttgga gtgaagacga cggaagcgat tttggaacgt
    14101 cttcgggaag ggcttcgcag caaggagttc aacgactctg acaaggtatg ggatgcactg
    14161 cgacgggaag cggtacggat tctttcgttg ggtgcaggcg gcttgacatt gcgttcgaag
    14221 ccgacggtag ttctgatggt tggcgtgaat ggatcgggta aaacgacaac gattggtaag
    14281 cttgccacgc aactcaaagc ggatggcagc aaggtattgc ttgcggcggg cgatacgttt
    14341 cgagctgcag ccgtgcagca gttgcaggta tggggggata gggttggagt tgacgtggtc
    14401 aagggcaaag acggagccga cgcgggggcg gtgatattcg atgcgatacg aaggggacac
    14461 gagcaacaag tcgatgtcgt gttggcggat acggcgggtc gtcttcacac gaagacgaac
    14521 ctgatggagg aactcaaaaa agttgtgaga acgatgggca aagcgttaga gggggcgcct
    14581 cacgaaattt tgttggttct ggatgccacc aatggccaaa atgccgtggc gcaatcccga
    14641 caatttgcag aagcgttacc tttgagcgga attatattga cgaaactcga cggtacggca
    14701 aagggtggag tgattcttgg aatctgtgat gaactcaagg taccggttcg atacgttggg
    14761 cttggtgaga aggccgatga tcttcggccg ttcgatgcgg aagagttcgt agaggcactg
    14821 cttggcggtg ctgaatctga ggacaatgaa gcagcgtaga aacaagaagg ttggcgagaa
    14881 gatggattgg gagttgattg ttgaaaactc ctcatgatat agtcacgccc gctttggcca
    14941 atggccgttt tctacaatgg ttctaacaac ttaggcctgt caactgcgcg aagaaacgat
    15001 gcggaagggc tcacaatgaa gcaggctcgc acaggactcc tcctgggcat ggcgctcttg
    15061 ctggtgtcgg caattggagt agcccaggtc aagaaggcga ccacagccga ggaagtggca
    15121 gatggtgacg atgttgaatt cgatgcggat gcgcctgagg agggcgagga tgatgtcgag
    15181 acggacgagg gcggtggtgc ggtagggttc gaggaaggtc ttggcgacat ttgcaaggtc
    15241 gaccccgatg cttgcccgaa gatcagtatg gatgaagcct ccaagcagga tctgaatccg
    15301 cagatctatg cggtgcagca gatatacgcg cttcgccgcg aacgctttga gctacaaccc
    15361 tattggtcgt tcacgatgaa tgatcagttc gtgtcccacc ctgcccctgg cgttgcgctc
    15421 aactattaca tcacgaacgt gatggcgata ggagccaact tcaattggta ccaagggttg
    15481 aatacggact cggaattcaa cgctgccacg cgccgggcgg tgcgagtggg tgtgccgctc
    15541 aatgagtacc aatggtccgc ggctctgaac tttacctacg ttccgatgta cggaaagttc
    15601 gccgggtttg gagacttcat cttccactgg gatggatacg tcgtaggggg agtcggagcc
    15661 atttcgactc ggccgatcgt cgtattcgat ccgcggtacc ggaacttctc cttcgaaccc
    15721 aaaattgcgt tcaatgcggg cattggtgct cgcatcttct tcaatcgctg gttcgcagcg
    15781 atcatagagc ttcgtgatta cgcctatctc gaggaactcg agaatacagc caactacgat
    15841 gcgatgttta gtagcgacga tgcacggacg gcttggcgtc aagacgaagc gaactggacg
    15901 gacgagaaga agcttaccaa caacgtgcaa gcccaggttg gcatctcgat cttcttgccg
    15961 ttctcgtggg agtaccggct tccgaaataa cgaacggtgg tgggaatagg tcggatgatg
    16021 gcaaagggaa ggacgagggc gacaatgcga cgaatcttcg ccaccgggct gctggcagtc
    16081 gttggcgtgc tcgcgatgga atcgactgcg agtgctcaag aaattcagct cacggggccg
    16141 ttggctggtg ctccagccgt gcgtgaactc cgttggcacc gtaacaagcg gttcgaaatc
    16201 gctccagcga tctcgtttac tctgctggat gaataccagc ggaccatttt tgctggtgct
    16261 cggttacaat atcacttgac agattggttg ggcctaggcg cgtggggtgc ttatggagtc
    16321 gtgcatctca acaccgcgtt gaccgatgaa atcgataagg tcaacaaaga tcgctgggat
    16381 agctcgaggg acaacgatgc actgacggcg gtcgaccgaa acgcatcatt gttgagtgtt
    16441 ggctacgact ttccacagca gctcggaaag atccaatggg tcgtagcccc tcaagtcacc
    16501 gcggtaccgt tccgtggcaa gcttgccttg ttcgaaaaag ccttcgtcga cacggatcta
    16561 tactttttcg ctggacccgc gttcattggc gtcgtcgaac gcgaggatta ctccgtggcg
    16621 gactttggca atccgaataa aaccgtatta gaaaacggga aacccgtaaa atcctatcct
    16681 accgcgtctc gagtagccat cgcgcctacc ttcggtgctg gtctgtcctt cttccccgga
    16741 agttggtccg gtattggtct cgagtatcga gcgcttcctt tttcgtggaa caactcggga
    16801 ttcgactccc aaggaggccc gcccgacgaa aacggaccgg atagcattgt cgatgaaaaa
    16861 gaccgccagt ttcgcttcaa ccagatgatc acgatcagct tcagtgtctt cctgcctacg
    16921 gacatcaaag tcagcccctg aacctaacgc gcggcaagcc agcttccacc aatcgacaca
    16981 ccccttgcaa agatgcgtgg cgaattgccg cttgctgtgc ctttgtcatt caccccacca
    17041 ttgctccacc acgacttccg gttccactcc gtgtttaatc gcaaactgcg ctcatgatcc
    17101 gacgcgtcgg attcgatatc ctaccgagtc gaccgcgccc ccgattgagt ctgcgctgga
    17161 atatggcagg tcttgagcgg cctgggcatc agttttttct gacgattccc tctcgaagag
    17221 cataaccggt gggaagggca ttcatgatgt cgttggaaag ctcagcgggg cagacccatt
    17281 cgcgagccag catcccttcc gcaattcgat tcttgatgcg aacggactct tcggaaatca
    17341 gtgcgcgcat cagggccgtg accgaagttc gatcttcttg agaaaacagc gtcgccacgg
    17401 tgtgaaaacg cactcgttca tcgaagtcat caagaaacct ttcgacggca acccgcacat
    17461 cgtctctttt ctttccctcg agttcggcga tgatctgcag cttgggttct tggtttttcg
    17521 agtaatccgt atcgaaacgt tccagcagca cgagcagttc atcgacaaat gcctcttcgt
    17581 ctagcagttc ttcgagcatt ttcatcggcc aggtgaagct ttccgccttc atacaaaact
    17641 cgcgaatcgg ctctacagca gccggtcccg cggcaatgat tccgcgaaag gcaatttctt
    17701 tttcttcttg atcggtgatc gacggctcga tgtagaaaga aaaacgtttc agcaacgcgg
    17761 ctgcggaatc agcggtcgca atggtgcaaa ggctttggat ggcctcgagg cggtcatagg
    17821 cctgtgcgtt tttgttggcg gccatgcgtc caagccgagc cacgttttta tcgtgtttcg
    17881 aatcgtcgct gtcttccgct ttgcttcttt tgaaaatatc gaacaggccc atgtcatgac
    17941 ctcgaggggt actgaggatt tggaatcgaa agcgccatag cacacgaaga cgtctccgcg
    18001 cgaggcgcat acgaggttag ctatgcttca tggaagttgg acagcgagca gattgacttc
    18061 tcgcccgcga agctgcactt ggtaacgaac cgggcgcccc gatttggcgc tttgctcggc
    18121 ttctcgctga cgaattcgtt ccacgaaact atctttcgta atgtgatcga cagcgtcgcc
    18181 gatgctgcgt ttggcattca catattcctg gaacagtctc gcgtagtact gttccgctgg
    18241 ctcagcagcg agggcgcttg ccaccgcggg atcgactttt tcggcggagg ccgccgccgc
    18301 gggggcgtca acatccaagg cgccttggaa agcattggtc gacggtgcaa gcgaaggcct
    18361 gccatcctcg tccgtatcat cttcttgcac gcccatgagt tggttgagca aggtattgat
    18421 tcgaaacacg acgcctccca gcacggcgtg ttcgatttca aagcggacat ccgtacgccc
    18481 attgagaatt tgcagcagtc cttcttcgag ttcttcaacg ggtttggaga tatagtttcc
    18541 gagtagccat cctgcgatac cgacgaggat gagacccaag acgaccatcc cgaaaatggc
    18601 gtagaacacg cctccaacgt ccatgacgga agccggtgaa acagcgatga ggacagcttt
    18661 ggtgccgcca tagcctccga gaggagcgcc tacataaatc cagtctttgg gaccgccaac
    18721 gagcttttcc gcggcatcga agcgcatggc acctttgacg ctttccgcga gggggccttc
    18781 cgctatggcg ttggcggcgg tcgcgtcgac cgttttcgac ttcgccgctt tttcgattcc
    18841 tttcggccct tcgacggcga caacgatggc ccccccactg gttttctcta tcgtccgagt
    18901 cattcgctca tcgttcaggg gcgtaccgta gatgacgctg ccgagcacct ggccggaggc
    18961 gtttcgtacg ggagcgaaag atacgagcca ctgttgagag aggttgggag cgaaccaagt
    19021 ctcggagccg gtctgcccat gaacgatggc ttctttcatt cctggatgcg ctttgccaat
    19081 atcctcacct cgcatgaggt tattgccatt gcgtccgagc gataagccat tgatgtcgac
    19141 gaaagcgacg acagccggat gaagccctgc cagtgctggt gtttgaatag cggcttcccg
    19201 caggccattg gcttgcttgg tagcagcctc ggcccgagcc tctcggacgg cgagttcgaa
    19261 tggttcgcgc aaggcaggtt cgttgacctg attcgccaac caacgctcca cctgaagggc
    19321 atcgagttgc agttgcgcgt tggccgccga tacggcttgg gaggcatcgg atacgatggc
    19381 ggcttcattc gcaaccaagc ttttcaactg agtccaaagc aaggcgctga ggaacacacc
    19441 aaggaggacg atgacgacac tgtttacggt gatgattttc cagcgcatcg ggctctttct
    19501 ccccaatcgg gcgacccccg gtaccaagcg caacgcaaac cactgtgtac atcaacaatg
    19561 gcgtacttgt gggaagaaag gtaccactgg cagaggccgg ggttcaagct tcgggtggaa
    19621 ggccaagcct agccgggaaa aaagaaagcc ggtctgcaac gcaaccggct cgaatatatg
    19681 gtctggagcg ggagacggga cttgaacccg cgacgttcac cttggcaagg tgacactcta
    19741 ccactgagtt actcccgcag gggagccggg tgagtacacg catggcgccg ccttgtcaac
    19801 ccaattttga acctcccggg cggtactaca tccatgcctg agcagaattg cacagccgaa
    19861 cgcagtcgcg gtatgatgtc ggacgactgg ggctagtccg tcgtggattg ggcacttcgc
    19921 gaaacccgtg aagggggagg caaacatgaa ttaccagcaa ggcaaggacg taatgttgtc
    19981 ggcggcagtg acaagcgaaa cgccaaccaa ctggggcttg ctgagggtca agggtgacgg
    20041 ctcgctatct ccgctgccat tgttcgatgg ggccattgtc gacgtgacgt gcgacgagtc
    20101 tcaccacgat ggcgatacga gtcatgtccg ccatcacgcg cagttcatcg ttcgttcgga
    20161 aggagtgtat gtatcggatt tggatagtgg ccaggggacc tgggtggatg gaattgccgt
    20221 acgtaagctc ggattggccc atggttcggt cttgaggtgc gggcgctgta tcgccttatt
    20281 cgtggagcga gacctggccc aatcagctag ccctccaaaa cgaaaaggaa atgtcgttat
    20341 tggagtccgg ctgcaagcat gcctggatac ggcacttggg ttcattcatg ggaaacgtag
    20401 ttttttggtc gaaggcaacg cgggtgttgg caaatcgacc atggtggacg cggcgctacg
    20461 gaaagcaggg gaaacaacgc ctgtttgtca tctagacggc gcttgtttcg agtcggaggc
    20521 gctcgtacga gcctttgaac gcacgtctcc ttgcgtgtgg gtcgtgcgtc actttgagca
    20581 gtttccacga gccgcgcagg cagaccttgt ccgcacggtt cgaatgatgg atggatcgtt
    20641 tttgatcggg acagtggtag gatccgtcga gcatgcacat gccgaaggac gcgtttgctc
    20701 atcgtgcatg acattgatgg gttctcacgt gatccgtgtt ccatccctca tcgagcgcag
    20761 agaagacatc cctatgttga caatggagta tcttcgaaga accgaggtcc cgatgacttt
    20821 tgatgtcatg ggattgttcg aatgcgtctt gcgagccggg tggacgggcg gtatccggga
    20881 actgcaggat catctatctg ccgcgttttc gtccgattcg gtatcggagg atccgatcaa
    20941 gacacttgcc cgaagcattc cccgtccatg ctcgaaagaa cctttgagcc tttcgtcatc
    21001 ggatcccgat ttggcgcgcg ttcgacttca aaaagcgctc acgatggcgt ccggaaccgt
    21061 cgccgaagcc gccagaaacc ttcgcatatc gaggcagacg ttctatcgag agcttcgtcg
    21121 attgagtgtc gaagcgactc gttcctgatc agacacgttc gacactgaaa acaccttgca
    21181 cttttcgcaa cgcccgcatg atgattttga gttgcgacag gtcagcgcag ggaaacgtga
    21241 agatgttcat ggcgcgtccg tcttcgacag ctcggctcgt gacggcagtc aggttgatat
    21301 cttggtccga aaacgcttgt cccatcgaag ccaatagtcc cgggcgattg acggtcaaca
    21361 ccttgagctg caccgagcga ttggctttga cacgagaatc ccaaaccacg gcgatgcggc
    21421 gatcggggtc ggtttcgaag gctttggtac aatcccggcg atgaaccgta atgccacgac
    21481 cgcgggtgat gaatccaatg atctcatcgc ccggcaaagg attgcaacac cgagcaaagc
    21541 gcaccaaaac atcatcgatc tcgctgactt tcagcccctg gacgtctttg cccatcaaac
    21601 ggcgaacgaa gtgttcgatc cgtccttcac gaacactcgc ctttggtttg agcttggcac
    21661 catcgtcgac agaccgagac aaggtttggg taacgctcgc tggagccact tttccatacc
    21721 ccacggcaat aaacaggtct tgcagcgtgg gtatctcgag ttcaccaaga acccgaccaa
    21781 gctcagcgtc gtctttggta aaccgttgaa gcgagaaccc gttgtcatgc aattcttttt
    21841 cgaggagttc ttttccgagt ttcgacgacc gctctcgttc ttcatttcgt aggtaggcgc
    21901 ggatccgatt acgagccctg ctggtcgcgg cgaattccaa ccactccttt ttcggccgtt
    21961 ggtccggact ggtgatcatt tcgatcatgt caccatttcg caacttatgt cgaagcgacg
    22021 agacggttcc gttgacgcga gcactggagg aatgatgtcc gatttcggta tgaatggcgt
    22081 aggcgaagtc aatggcggtg gcacctttcg gaaatacgcg agcctcccct ttgggagtaa
    22141 atgcatagac ttcgtcttgg aaaagatcga ttcggatgct gtcgaggaat tccgccgcat
    22201 ctttgacttc ccgttgaacg tcgagcaaat catggagcca ccccaaccgt tgcatgagtt
    22261 tggggtcggc ccctccggac agacgatctt cataaatcca atgggcggcg ataccttcct
    22321 gggccactcg gtccatttct ttggtgcgga tttggatctc gacacgttcc cgacgcgtac
    22381 cgatgaccgt ggtgtgtagc gattggtaca tattcggctt gggtagtgcg atatagtctt
    22441 tgatccgccc cggaatcggc gtgtaacgcg agtggatcgc ccccatgacg ccataacaat
    22501 ccgctaccga ttccatgatg acatgaaaag ccacgaggtc acgaagctgt tcgaagtcga
    22561 ggttgtattc gaccatctgt cgatgaatcg aatacaggtg cttcgttcgt ctatacacct
    22621 ccccggcgaa accgtgatca tgaagctgct tacgtaactc ctcgcagatt tcacgagaaa
    22681 accgctcacg caccgctttg gtttccccca tcagacgaga tagattttga tgagcctccg
    22741 tctcgaggaa ctgaaaagac aggtcctcga gttcggtttt gaaccgatga ataccaagac
    22801 gtgccgccaa gggagcaaac acctcacgcg tttcacgggc gatacgttct tgggaatcgg
    22861 gcttcatgaa ctccagggtt cgcatgttgt cgacgcgatc acagagttta aggagtaaaa
    22921 cccggatatc ccgcgacatg gcgacgagca tttttctcag attctccgca tctcgttctt
    22981 gacgagagga gaactcgagt ttggacaatt tggtcacgcc atcgaccagg aaggcgactt
    23041 ctttaccaaa tactttctcg agatcgtcga cgctttgggc tgtgtcttcg accacatcat
    23101 gaagcagacc tgcgcataca caggcggcat cgaggcgcag ttccgtaatg acgccggcga
    23161 cagcggccgg gtgagcgaag taaggatcac cgctttttcg cacctgccct tcgtgggcgg
    23221 aattggcgaa gtcgtaggct tttcgcagca gttccgtatc cgccgttggt tggtaggcga
    23281 gcacacgctc gatcagtact tcaagcggga gggtgccagt gcgtgagctg ggaaggttca
    23341 aagccaagtc tctgagggca accagttata aggtatcatc cacggacaaa acgggcaacg
    23401 ttacggctat cgttcctctt tggaacgtgg cgggaggacc gaagtgaagg gcgaagcgca
    23461 agaacgaggt tgcatggcac cgatcaatgc taagctctcg tgcccgatgc tgctggtccc
    23521 gctcgggaca gcgtgaagtg tggagacttc ggatatgatc gacgaaataa tcggcgacct
    23581 aaataattcc attggcaagt cccaagaagc gttcaagcgc gacttgagca agcttcggac
    23641 gggacgcgcg catgctggca tgctcgacag cattcgcgtg gattactacg gcacggcaac
    23701 gccattgaat caacttgcca ccatcagtgt acccgaacct cggctattga ctgtcaggcc
    23761 gtgggacaag ggtgcggtca aggccgtgga taaggcattg cgagaaagcg atctgggcat
    23821 caaccccctg ctcgatggtg atgtcattcg gatcccaatt ccgcaattga ccgaagaacg
    23881 acgcaaagag ctggtcaagc tcgtcaaacg catgggcgaa gactgcaaag tcgcggtccg
    23941 caagcaccga cacgatgcca tggacttact cagcagcctg caagacgaag gggaggcgag
    24001 cgaagacgag gtaggacgcg cacgaaagcg agtcgaagaa gtcgtcgccg aggccgttcg
    24061 caagatcgac gaaatccagc aagccaaaga aaaagatgtc ttcgagaact aatcctggct
    24121 tgctcagccc ttgacattcg gggctcgggc atacgaaagt gaactttcat cgatcaattt
    24181 cggaatatct gcatgggtaa gccgaacccc cctcatcgaa tgcgaatcgt tgcttctgga
    24241 ttgacagacg taggtctgca acgggaacac aacgaggaca gctttctcgt tctcgaagaa
    24301 cacgatctat tcatcgtggc ggatggaatg ggaggccatc gcgcgggcga cgtagcgagc
    24361 cgcgtagcca ccgaaacgat ggctgagttt tttcgctcta ctgcgaatga cgatgtcacg
    24421 tggccgtatc atttcgacac gtccctttct gaagaagaaa atcggctatt gacggggata
    24481 cgactggcca atcgacagat atttgaacga agcatccgct ctcgggaata ccacggcatg
    24541 ggcacgacga tggtaagtgc tctgttcgct cggcatagcg gcaagctgta cattggtcat
    24601 gtcggtgata gtcggtgtta tcgcttgcgc gagggcgagg tgcgcctgat gacccgcgat
    24661 cactcgctgg tcaatgacta cttgctggcc atgccagagc tttcggaaga tcaaaaaggc
    24721 gaacttccca aaaacgtgat cactcgagcc ttgggaatgc aagaccatgt ggtggtcgac
    24781 ttgcagtgcg acgaacccat cgaaggcgat atttacgttc tatgctccga tggactgtca
    24841 gggatggtgc ccgatgagtc tatcctcgaa accctgagcg acagcaccga tcccgaacag
    24901 atatgtcgtc gcttgatcga aaccgccaac cacaacggcg gagaagacaa tatcacagcc
    24961 gtcgtgatgc gcctggtcgc cgacgatcga ccggatgatg aacctgaatc agagccgctg
    25021 gacgacctct aacgctggtt cgaaaaaggg cgttgcgcgg ttaggactcg caattctcga
    25081 agaagagata tccgttcacc aacgaacact tctcgttctc ggaagagcca gtttcgatca
    25141 gagggcactc gaaactaagc caaattttcc cttcctcacc ttcgatcagt tgaaagttac
    25201 aaggagattc ggcgcttgct ccgtatgact ttcccgccgt attcgcccca gccacgaaaa
    25261 tacgtccttg gttcgagttg ccctcaccca cggctgttgg aacgagacgg aaccgggtca
    25321 agcctttgga aatgcttccg ctcgctacaa atccttgacc ttttttaacg accgaacact
    25381 caatcgtcgc gtcatcgtcg ccgtccgaaa tggtggcatg ctgggccggt cccacggcac
    25441 ccacctgaag caaactcggt tgcatggccg gacaggtaac gctcgagcca accggattgc
    25501 caaaggtcac cgtgaacgct ccttgaggca cgggagaaga agggtcgcta catcccccaa
    25561 gcccaagaac cgaaatgagt gcaaaaccac ttccaaggta ccgcgacacg ctcatggtct
    25621 tttccttctt ccatctcagc atacaatagc tgccggacaa gactagctca cggctggggc
    25681 aggtcgcaaa tacctgtcga tgaggatttc agcaggaagg tgggtaatgg ggtgaccgac
    25741 gggatttgaa cccgcgacga ctggagccac aatccagtgc tctaccgggc tgagctacgg
    25801 ccaccgtgtc gatgggcggg acatattact cttgtaattc cgaaacgcaa cgtcagaacg
    25861 ctgctcgaac caaagcttca tgataggttg caggcgtagg cagggtgaat ctgggatatt
    25921 ttggatagta ggcgtaattg aatgagccaa cgcgtcgctt tcatgattgg ggtcatggtg
    25981 ctgatggtcg ccctgggggg gtgccaaaaa cggct
//
LOCUS       DAOSOR010000009        25898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_1897_length_25898_cov_5.393530, whole genome shotgun sequence.
ACCESSION   DAOSOR010000009 DAOSOR010000000
VERSION     DAOSOR010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 25898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25898
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1897_length_25898_cov_5.393530"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PK710_00985"
     CDS             complement(<1..195)
                     /locus_tag="PK710_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOT08304.1"
                     /translation="MAYSNKVIDHYENPRNVGSMDKNDDNVGTGLVGAPACGDVMRLQ
                     IRVDDDGKISDAKFKTFGCGS"
     gene            complement(286..1509)
                     /locus_tag="PK710_00990"
     CDS             complement(286..1509)
                     /locus_tag="PK710_00990"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HOT08305.1"
                     /translation="MAVNLPIYMDYSATTPVDPRVLEEMMPYFSEKYGNAASRSHAYG
                     WTAEAAVEAAREKISAFLGGSSGKEVVFTSGATEADNLALKGVADYYASKGNHIITTT
                     IEHKAILDTAKRLRKQGFEITYIPVDRQGLVDPDEIRKAITDKTILVSVMLANNEVGT
                     IQPLEQIGAITREKGVLLHTDAVQGLGKIPFDVTRTHADLVSISAHKMYGPKGIGALW
                     VRRSKPRVRLVAQMDGGGHEFGMRSGTLNVPGIVGFAKAVEICEQEMAQESERTKTLR
                     DKLRHGIQDHLEEVNLNGSIEHRLPGNLNLSFAFVEGEAMMMAIKDVAVSSGSACTSA
                     SLEPSYVLKAMGVGDELAHSSIRFGIGRFTTEEEVDYVAKLVVEKVRKLREMSPLYEM
                     YKEGIDLKSVQWAAH"
     gene            complement(1513..2124)
                     /locus_tag="PK710_00995"
     CDS             complement(1513..2124)
                     /locus_tag="PK710_00995"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOT08306.1"
                     /translation="MNQKSGQHDTEDKKAQNRRLSVIDEPARAQSSRDRETGEPTLLR
                     VGDLAQETGKTVRAIHLYEEMGLLRPRSRSKGRYRLFGPDAVIRTRWISKLQELGMSL
                     SDIRAVIQEWENLSAPGAMIRMREVYRKQLADTRSQLERLRVLERELASSLAYLESCD
                     ACEPDRLITECQQCALHDDDIEPPDLVAGLSAHVSPCPGAERE"
     gene            complement(2622..3623)
                     /locus_tag="PK710_01000"
     CDS             complement(2622..3623)
                     /locus_tag="PK710_01000"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOT08307.1"
                     /translation="MLRNVMIGLSFALALSACETTEENPTPQEAGASDSHSDFDAGSD
                     AVGEDSADAATDTVSADVENEAGLVVPEGYVLVSPAVFIMGSPEQEPGRADTETAHEV
                     TLTRSFVMKKTEVTQGEWQSLMGYQPSYFEGCESCPVENVNWWEALAYCNALSEKEGL
                     APCYALEGCSEPGPDFLCDKVTTTGGGLLQCEGYRLPTEAEWEFAARAGTTTPLPQGS
                     IEKDPSSCEVDDVLSDYGWYCGNSSDEPHPVGEKSPNAWNLSDMAGNVMEWVWDCLVP
                     YGPEKVNDPIVNEGSGMRVLRGGSWSQAIGQCRSAARAGLDPAERDGDTGLRPVRSF"
     gene            3790..6018
                     /locus_tag="PK710_01005"
     CDS             3790..6018
                     /locus_tag="PK710_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HOT08308.1"
                     /translation="MTSDVTLFASHLGDPEATQAVPKEELTLLRRVLHHLEERRAAIP
                     ALQTYDAELISLRDQVSEARLEDVPALLAQMERLAGISAQRNELRVNLVDPGNPYFAH
                     LAMREQDRSGTRDREVLIGRTTYIDAASNIRIVDWRHAPVSQIYYRYAEGDVYEERFG
                     DREVEGELLIRRTVTIHKGSLVRIATPQGTYVRQQGDDFRLIPPRAADLSGGQGKATR
                     PAEAKSRGKLGVGPDGQQRIDRHLPEISALLDPRQFDLISRTDSGIVVIQGGAGSGKT
                     TIGLHRLAFLNYQGPKKFAGERMMVITTSKGLVSYTSEVLPALGVRDVQVHTFPTWAQ
                     RMRRIHFPWMSDVEVTEDTPLSVSKLKKHPGILRAIERRSQLAQEDPKLRSDPHAPVT
                     LWAELLTDLDAVRQAMLLSDAPALEDAEIQQAVRWCSDRCPLVAELRPGEPRAEPAAP
                     PDEEDDLRMDVGIDGLPTTEDHLTLDLEDDALLLRAYQILRGPLRKGKEPIQVEHLFV
                     DEAQDFAAAELAVLIDVTTERRSITLAGDTSQRLVMDNHFRGWEGMFRDLGIEAIAIE
                     PLKIAYRSTREILELAKDVLGPMADALPSVAPRTGAPVEMFITPGPGVAVAQLGEALR
                     ALALREPRATVAVLARYSDQADVYYDGLRRAEVPNLHRVKEQDFRFRPGVDVTEIREV
                     KGLEYDYVILVDVNASTFPDDIESRHLLHVAATRAAHQLWLVATGPESTVLPHWLTDT
                     AL"
     gene            6080..6250
                     /locus_tag="PK710_01010"
     CDS             6080..6250
                     /locus_tag="PK710_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08309.1"
                     /translation="MATKRHYKDWYSYETRQTSWLEAFWMIVFTVVLVIGFPLMFAAM
                     FRYAVPLLFGLL"
     gene            complement(6337..6540)
                     /locus_tag="PK710_01015"
     CDS             complement(6337..6540)
                     /locus_tag="PK710_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08310.1"
                     /translation="MLRKAAAAGLKLLILALAVYAFFFLPLGRRTPYQHLNAIFSSQP
                     AREAAEDLTVAGQQIKNKVREMK"
     gene            complement(6594..7565)
                     /locus_tag="PK710_01020"
     CDS             complement(6594..7565)
                     /locus_tag="PK710_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HOT08311.1"
                     /translation="MQNPVSKGPPPPPGSSQNRPLALRFISGKYQGGEFPLVPGKEIH
                     IGRSNDLDMVLAEDMVSRKHARIAFQGDDIFIEDLASTNGTFVNGEKIKRSKLREGDR
                     VLIGTSILKVVISESVRRTSEEQARRDMEIVAAHRRTSQVRSMSGSIEEIPLPDLLQL
                     LGTSKKSGVLVIRTEEDVIGRIYMRKGLVYFASINDMDEIHPFKSVFRMLSWTNGLFD
                     LEPPDERTFANPLDMPVQGVLMEGMRQMDELNQIKERLPRLTSRLHMAMPLQPALRDL
                     KPQELDILQLVHNFGYFEQVLNQSPSSDLDTAQAILKLIDNGYLRVD"
     gene            complement(7803..9917)
                     /locus_tag="PK710_01025"
     CDS             complement(7803..9917)
                     /locus_tag="PK710_01025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT08312.1"
                     /translation="MDLRTRTIMLCAAFATAITVSIMLRSRKNRTHYLLSAFAGTVAL
                     WYLAQALFGYFQADIWYRFTAVLAVLLPQFALHLFEAIVPRPANEHSKLLRVGMVLGI
                     PMLLLAISDYQTHAAVRTTIFLYVFGLVASGLAGLAMQGRKAPSRAVRERVRLLVLVG
                     TFALFFSIGDFVWFIGAAIPPVGAVLSVVFLFALAESMQRERLIDLYEMVGRLVVSTA
                     LAFCLAGIFYVFVTYVGFDTMYLNAVLAAIAILVLFEPLRNKVEEKIHVVFFRQRHEL
                     ERVLRDAEMRLIHILELEEMASVVIHALEESRRATSAALYLLDPDGRELSLLHRFGLG
                     AEERIEVATARPLLERLRQADSVVIDDYALSSWSLGFHGDNLTGRDALLTAARLVGPI
                     ANSGVILGIRPGSGDVIGLLVVRDERVRDAFSRDEIVLLERLASMIGVVVENTRAYAR
                     MKERDRLAVLGQMAAGLAHEIKNPLGSIKGAAQLLIDSVEDQEGPVHSQDFLDIIIEE
                     VDRLDRVVGSVLDYARPRKGNPAPVDVNAVVKRTVQILSPSSHERTRLEVDVAPDLLP
                     VRIDAEQLRQVIINLVQNAMQAMGERGGVATISTRMREGEPGSLGWVEIVIKDTGPGI
                     SSEMLNSLFVPFFSTKAHGTGLGLAISQRIAQEAGGVIEAASQQGVGSAFKVVLPACS
                     QESVPCASLRKEPSEAAGGITG"
     gene            complement(10012..12378)
                     /gene="cysM"
                     /locus_tag="PK710_01030"
     CDS             complement(10012..12378)
                     /gene="cysM"
                     /locus_tag="PK710_01030"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HOT08313.1"
                     /translation="MTDDTTQPRMIPPREGVLARIGNTPLLRLRRINPNPNVEIWVKL
                     EGNNPGGSIKDRTALWMIEQAEKTGELHPGKTIIEATSGNTGIGLAMVAAVKGYRLVL
                     AMSEGASVERRKILAAYGAELLLTPAEKSTDGAIEVAYELAAQEPDRYFLADQFNNPA
                     NPLAHYESTAPEIWEQTGGRITHFVAAVGTTGTLMGCSRRFRQLNPKIRIIGVEPYLG
                     HKIQGLKNLKESYVPGIYNAGALDEKINVDDDAAYEMARRLAREEGLLVGMSAGADVH
                     VAVELAKQMESGVIVTILCDGGERYLSTTLFQVAQPDVVPARLSFLNTLSGRYEPFVP
                     LSSSDDVTMYSCGPTVHRRPHLGLLRRMLVDDLVRRTLEHAGYRVQHVVSVTDVDDNT
                     VQESERTGEPMAELCRRHEQGFFEDLDALKISRADQYVRASESVDAMIALTRELMDAG
                     VAYEKLRSVYFNIGRVPSYGELSGKDLGKIRMGATVDLERYDKNDPRDFTLFRRSTLG
                     ELRKGVSYKTEWGNVRPSWHVQCSAMARATLGDRFDIHMASVELVFPHNENEIAQIRA
                     LTGKPQAQFWLHSELVLVNGKKMTHEEGCVTLQDLIDRGYSAREIRFFLLRHHYRQPV
                     WLTDERLEEARASLRRIDEFVSNLQEVRTEGEHVEEVAGWVVGMKETFRASLYDDVNL
                     PAAIAGIFRLIRQVNHVMGQGKLCEACAKLVLEGLTEVDRVLSILPALEPQQAIPQEI
                     ASLLKERDEARSRKDFARADEIRALLQERGWVVEDRATGLRVRRKEDR"
     gene            complement(12375..14135)
                     /locus_tag="PK710_01035"
     CDS             complement(12375..14135)
                     /locus_tag="PK710_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOT08314.1"
                     /translation="MKQLRFRNLLRRHVVGRHTEVWHHRSFRLPLSAVGGLTGFEPRR
                     ADLVLWFLADAWAIEEGMLERPNRVRYRDLARVHTQEYLESLSQPRTLARIFAADETE
                     LQVDEVLKTIRFACGATVQAARSRLVHGGIALNLFGGFHHAAPDSAGGFCAVNDIAVA
                     TAVLRNEGFRGRIVVIDVDAHPPDGLAACFRGDSSVWVGSISGESWGPLEGVDETVLP
                     RGSEDRTYLCALDRMLSRMPRADFAFVIAGGDVVAGDRLGKLALTLEGARRRDRMVLE
                     ALGRVPSVWTAGGGYGRESWKVLAGTGLMLTCRSSTAIPPSYDPMRARYGWVSTKLSR
                     ERLEGAEELSAKDLEEALGLRPPEQPRLLGLYTAEGIEYALSHFGIIPHIRRLGFEQV
                     HAAIDVVDMGDRVRVMGNALGKQHVLVECMLERRRVVERDVLYVHWLALCNPRARFSE
                     KRPQLPGQEVPGLGLAREIGWALARIARRLGLEGVAYRPAWYHTAYSGRYHFVFADSR
                     RQGRFEAMQRDLRDIPLIEVTRAAAEGRLRNHGERCTWEAEEMVFWLFDSPLDEKIVA
                     EERANARFTIVASECDKHVP"
     gene            14365..15306
                     /locus_tag="PK710_01040"
     CDS             14365..15306
                     /locus_tag="PK710_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08315.1"
                     /translation="MNKYLSIFMYLSLGSCLVGCALDTVADELSSEQVDATEADLSTN
                     AVTFVTVRKDNRKCAAPSCGGYWVKDVNRSSGEQYVSGFDFGSSGFDEQLIGKVTDAP
                     ADELILRGRLGKKEAKHGTRKLLVYQAFRGLPGVKPLPGDVYFQAETRDVPIQCFTAP
                     CPNEIAHRLNTNQQYAFSGYQVALAARPHVDQDWLVDRIQHHKAVVAALFVKGDKYPG
                     GYEYLLDASQVYLDIADAVGPCPAYKLAACSEPDADWAYTRDVNLCLLPEVCMSTLGC
                     PSLQPPRCEDGYSVSAWRTNSPSCIRFACDPSFVLPN"
     gene            complement(15503..16657)
                     /locus_tag="PK710_01045"
     CDS             complement(15503..16657)
                     /locus_tag="PK710_01045"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOT08316.1"
                     /translation="MSLKVLVLNSGSSSIKFELFEMPSEKRQARGLLEKIGEEQSKLT
                     YTIGDSKYTIDQKVADHGQGLRLIIDALTDQSKGGLKDISEIGAVGHRVVHGGEAFSE
                     TVIIDDKVQKAVEEHIQLAPLHNPPNLLGIKVARELLPNVPQVAVFDTAYHQTMPAYA
                     FTYPIPYELYEQLRVRRYGFHGTSHRYVAARAAKILGRKLEETNLITAHLGNGASMTA
                     IQNGKSIDTTMGLTPLEGLIMGTRCGDIDPAIIAFLARVKDMPVAQIDNLLNKKSGML
                     GVSGLSNDMRSVEQAANEGNTRAALALEMYCYRIKKYVGAYTAAIGNVRACLHRRRWR
                     KQRCRARTRVFQPPKHRLQNRRGKKSRCSRKRTRHRHGRFPHSYPHHPHE"
     gene            complement(16664..18796)
                     /gene="pta"
                     /locus_tag="PK710_01050"
     CDS             complement(16664..18796)
                     /gene="pta"
                     /locus_tag="PK710_01050"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOT08317.1"
                     /translation="MASSILVTATQRGSGKSTLALGLVHLLESTLSRVGYFKPIGQRG
                     MDGTDPDVRLMKDALGLEFSLEEMVPVTMDQVQEALSNGTYDHLLDTILEAYEKIASK
                     CDFVVCEGTDYFGAMSAFEFDINADISKNLGSPVLLVANGDNCVGLTDDCECGPCDQM
                     IQNITMVKESFDAKGCEMFGAVINRADPKYVKTIHDAAEPSLRKMGLKLLGTVPRTDM
                     LSKLRIEEVAAALNAEVIAGQDRLNVIVQEVAVAAMSVENVLERIPRGTLVVVPGDRD
                     DVLLGLAGAFASPAIPAPAGIIMTGGLEPGKNVKHVVLDLTGGHMPLLKVQTGTYETA
                     ILVNQVKPSLQAHQKMRVELVKTLVESNIDVEPLIARASVQPRSRRVTPKQFIHRMMD
                     IARRDKKHIVLPEGHEPRILRAAQVLLERGIVNLTILGNEDEIQREISRQGLKFNGDL
                     QIINPATSELRERFAKRYIELRSPKKTPTWELSYDLMNDTTYFSTMMVQEGLADGMVS
                     GSITTTAATLRPALEFVKTKEGYSIASSVFFMCLPEQVLVYGDCAVNPNPTAQQLADI
                     AIASAETAQSFDIEPYVAMLSYSTGSSGSGSDVDKVREATAIVKERNPDLLVEGPIQY
                     DAAIDPTVAKAKLPGSRVAGAATVFIFPDLNSGNNTYKAVQRAAKAIAIGPVMQGLRK
                     PVNDLSRGCSIPDIINTVAITAIQAQRS"
     gene            19309..20904
                     /locus_tag="PK710_01055"
     CDS             19309..20904
                     /locus_tag="PK710_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOT08318.1"
                     /translation="MAGERGHEIAAPASPPYLTVFLLDGARHDVVFRELRSGRLPHIQ
                     QLIEQGALVENGVTSFPSMTGYAYYPLVTGHDSSRSGVLGLRWFDRSRDFGNLRSYVG
                     TTNDQMSADFAPRPRLLYERFPHQHSYSFNTYANRGVKKNVKAGFAYARAKYHRRFAV
                     LSALGRLPMLGKFLMPDWEEAEAQGLETALRDLKKYPKIQWVTIASMDAHHHVAGTDD
                     RYAELIRYADVLIGRYRDTSRALGQETDRIYLITTDHGAADASDNLDVREVLASCCGL
                     KAQRDRSTHLMGAALDQPLSDWFGNDAVVVINGDMVNYLYFSDPERSGAESWRHPMKW
                     DRLRSFKGKDVVVSLSQQPGISLVLGWSDEQQAAKVFSSRGEATIRTQPEGLSYSWQG
                     QDPLEYEAVARVRPLLDGKPHDDRAWLDATYGTRFPDAVFRIVRLLTAPGAPDLVLTA
                     AQGWDLGKDYEVLVGNYRGGHGGLRAEEMLTWFVLAGPGIRKGVRLATARTEDVGATL
                     FPLLGILPDPEATGKILEEVLER"
     gene            20901..23354
                     /locus_tag="PK710_01060"
     CDS             20901..23354
                     /locus_tag="PK710_01060"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOT08319.1"
                     /translation="MTDPTTTPRTISARLIAKLESGLAFLSLLCFRRPWIALGILALL
                     TVGAAQAARQRLSLNADLAELLPSSFESVKDLETLKQRYGGIGYVGVVASHAEPDVLR
                     RFVDDVAPRVDALASVRSVDYRKPTQFLRDHALYYLDLDDLKIVHNRLRTRFDFEVAK
                     RNPMMLDFDDEEPPSVDLSDIEKKYEARSDQAWMRTQLDDEYYIDVEKRRIALLAKPS
                     KMSVDLAFSRQIVGEIQQVIQQSEPTHYDPNLQVQYGGNFTKKVDQQDMIEKDLNTGT
                     IVALVLMVAYLLLHFRRVGAVALIMIPLVMGLSWVFGFAAIVYAQLNILTGFIGAILL
                     GLGVDHGIYLLGRFREQWQGRDAAEDAIRIAFGNTGRAVLIAAFTTAVGFAGLGMSEF
                     RAFREFGVIAAFGLLLVVVAYLTCMPALLAVAVRMGWQPRSAKAVQQSLYARLLAKRP
                     GLLLTVFTAFLVFSMPWVSKLEFNTNFRALTAANLPSFQLDSVIDGLLGHSQTPVVVL
                     TENREQSRFVATTLRERKAASDSSTIHLVATGDDILPDQQMEKQTIIQDMRKMIDRLA
                     SEKMGPDDRRKVDRMKRLVAAEPFDRQQLPDALGRMFFPDRKDVGDFVLVYSSVDLSD
                     GANVSRFAKEVRGIELPQGGHASAAGEPMILADIFDLVMQEGPPVLIGTVLLIFLTMW
                     VLMGKFWRACMALFPAMVSLVATFGLLGLLRIELNYLNIVLIPVLVGTGVDGGVHMLT
                     RGASLDLVNAADRASVPIFGALATTALGFGTLLMAHHLGLNSLGSLAVVGLAANLFAS
                     LVGLPSLLLVVQRWRATRA"
     gene            23436..23630
                     /locus_tag="PK710_01065"
     CDS             23436..23630
                     /locus_tag="PK710_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08320.1"
                     /translation="MSLEISWWLLALIVGGSAPLWIRGLTRRWEQRSQQRTERILERL
                     KQLQPSSNTSPTAEIDRNES"
     gene            23847..24674
                     /locus_tag="PK710_01070"
     CDS             23847..24674
                     /locus_tag="PK710_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HOT08321.1"
                     /translation="MERLFGFIGNRSDLGARVLDVDAPALATSAEGRRLGWGVGFYQA
                     GEVLLRRRPMDDRQVIAAASLTRDLRTDALLGSVRAPSFGEPRTQNTQPFRYRDWLFV
                     HHGTVNAFVRLRERLMESIPDFLRGNIRGETDSELVFYLFLSFLHDAGKLRGPTDPAV
                     MTEALRGTCTLLDRLAMEEGADDAGVMGILVCDGEHIAGLNRNAHLAFRLVHGENDID
                     ALLPEDALRNRRLVDMARVRFTIIASGLTEPRVGWSLLPTPCTITTSRLDEPMVESF"
     gene            24674..25333
                     /locus_tag="PK710_01075"
     CDS             24674..25333
                     /locus_tag="PK710_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08322.1"
                     /translation="MGSSAFRSKHTIPLMAAAVMVMPACGAVAVSSFETAAALPKSPR
                     RPDAVAIDRQSPSPPIQSTADTRDGLVTLQEPVDITNAMQTVRIFFRAVLHEDLEAMS
                     SVLTSDAQQYSSASGNGMPMNRHWERRFQKFDYTAAGQLPFRESMTQVYRYQDLMVPL
                     PGRPSRLENMTSQDILIRVPITRTRFGLDRVFGSEMYFLLQRQGREFLIRTLIEDFVV
                     P"
     gene            complement(25343..>25898)
                     /locus_tag="PK710_01080"
     CDS             complement(25343..>25898)
                     /locus_tag="PK710_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08323.1"
                     /translation="GGGGGGGGGGGGGGGGGATGGSAGEAGAAGNAGSAGSGGATALC
                     EPFAVDLHVADTWYFTLSDTESHVQEPSAWDFKLAKSGKGPWITLGAGVEAINLGNGD
                     SFLDVIEAPDAGFDGDPDLIGDTWRSGGAGETGYTMSENIYVLKLDDGTYAKLTVTMA
                     KAGKVAFDAFHQSDGSRNLVCTMP"
BASE COUNT         5495 a         7570 c         6954 g         5879 t
ORIGIN      
        1 agagccacag ccgaaggtct tgaacttcgc atccgatatc ttgccatcgt cgtctacgcg
       61 tatttgtagg cgcatgacgt caccacaggc tggcgctccc acgaggcccg tgccgacgtt
      121 gtcatcgttt ttgtccatcg acccaacatt gcgaggattc tcgtaatgat cgatgacctt
      181 gttgctatac gccatgaggg atcctctttt tcttttgatg cttcattggc ggattggatc
      241 cgcatcagca acgattcttc gtgatgggca acacggctcg gttaatcaat gagctgccca
      301 ttgaaccgac ttgaggtcga ttccttcctt gtacatttcg taaagcgggg acatctcccg
      361 gagttttcga actttttcca ccacgagctt ggccacataa tcgacttctt cttccgtggt
      421 gaagcggccg attccaaatc ggatggatga atgtgcgagt tcgtccccca cacccatggc
      481 tttcaggaca tagctgggtt ccagactggc gctcgtacaa gccgagccac tggaaacggc
      541 cacatctttg atggccatca tcatggcttc gccttccacg aaagcgaagc tgaggttgag
      601 attgccgggc aaccgatgct cgatcgagcc gttgagattg acctcttcga gatggtcttg
      661 gatcccatgc cgaagcttgt ctcgtaacgt ttttgtgcgc tcggactcct gcgccatctc
      721 ctgttcacag atttccaccg ctttggcaaa gcccacgata ccgggaacgt tgagcgttcc
      781 cgatcgcatg ccgaactcat gcccgccacc gtccatctgc gccaccaggc gcacccgtgg
      841 tttggagcga cgcacccaca gggcgccgat accttttggc ccgtacattt tgtgtgcgga
      901 aatcgaaacc aagtcggcat gcgttcgagt gacatcgaag gggattttgc ccagcccttg
      961 caccgcatcg gtgtgcagca gcacgccttt ttcacgtgta atggcgccga tctgttcgag
     1021 cggttgaatg gtgcctactt cgttgttcgc gagcatcacg gacaccaaaa tggttttgtc
     1081 cgtgatggct tttcgaattt catccgggtc cacgagccct tgtcgatcga ccggtatgta
     1141 ggtgatttcg aatccttgtt ttcgcaaacg ctttgccgtg tcgaggatcg ctttgtgttc
     1201 gatggtggtc gtaatgatgt gattgccttt ggaagcgtaa taatcggcca ctcccttgag
     1261 cgcgaggttg tccgcttccg tcgcacccga ggtgaacacc acttctttgc cgctcgatcc
     1321 tccaagaaat gccgagattt tttcccgagc cgcctctacc gccgcttccg cggtccaccc
     1381 ataggcatgg ctacggcttg ccgcgtttcc gtacttctca ctgaaatacg gcatcatctc
     1441 ttcgagtacg cgcggatcca ctggtgtggt cgcgctatag tccatataaa tcggtaggtt
     1501 gaccgccatg gatcattccc tctccgcgcc ggggcacggc gatacgtgag cagataaacc
     1561 cgccaccaag tccggtggct cgatgtcatc gtcatgcaaa gcgcactgtt gacactccgt
     1621 gatcaatcga tccggttcac acgcatcgca cgactccaaa taagcaagac tcgaggcaag
     1681 ttccctttcg agaacacgca gccgctcgag ctgggatcgc gtatccgcaa gctgtttgcg
     1741 ataaacctcc cgcatccgga tcatcgctcc gggcgctgat aggttttccc attcttgaat
     1801 caccgcgcga atgtcactga gcgacatccc aagctcttgc aacttcgaaa tccatcgcgt
     1861 ccgaatcacc gcatcgggac caaacaatcg ataccgaccc ttggagcgac ttcggggacg
     1921 aagaagtccc atttcctcat atagatgaat ggctcgaacc gttttccctg tctcttgggc
     1981 caagtctccc acacgcaaca aagtcggttc ccctgtctca cgatcgcgac tagattgcgc
     2041 ccgcgcaggt tcgtcgatca cggaaagacg ccgattttgg gcttttttgt cttccgtgtc
     2101 gtgttgtccc gatttctggt tcatcgaacc cttacgtatg cgtgagcgtt gttgggcata
     2161 tatggcccga atgccccttc gtcaagaccc gagtgactaa cagacctgcc agggtcaacg
     2221 aaaaagatat tcgtgataaa tacagtaaat tgaagaaata gcgaactgcg tgtggtttct
     2281 catctcccta tctcccccgt gcgtcgctgt ttcgtgccac ggccgagccg aagggctatt
     2341 ccaaggccgg gtcgaatcgc tcggggcggt tttgcacaca catggctttt cttcatcgaa
     2401 agctgtttag cagcgcatca gctcatgctc ggatgttcgt ttgctagacg aatggctcgt
     2461 tcgagcggcc acggccgact cgtgggaagc ggtgctcgac aagtgaatta tcgatgaaaa
     2521 atcagtaggt tacgcgtgtc acggaccagc aggtttccct ctgggtgcaa gtatttttgg
     2581 gggaggcttt ttgcaggctt tgcagcgggc acaggtgggt tctagaagga acgaacgggg
     2641 cgtagtcctg tatcaccatc ccgttcggcg ggatcgagcc ccgctctggc cgccgaacgg
     2701 cattgtccaa tggcttgtga ccatgagcca ccccgaagaa ctcgcatgcc agagccctcg
     2761 ttgacgatcg gatcgttcac cttttccggt ccgtacggaa cgaggcaatc ccaaacccat
     2821 tccatgacat ttccggccat atcggagagg ttccaggcat tcggcgattt ctctcccaca
     2881 gggtgaggtt cgtctgagga gttaccgcaa taccaaccgt aatcgctcaa gacgtcatcg
     2941 acttcgcaac tgctcgggtc tttctcgatt gaaccctgtg gcaaaggcgt tgtcgttccc
     3001 gctcgtgcag caaactccca ttctgcttcc gtaggcagac gatacccctc gcactgtagc
     3061 aaaccccctc ctgtcgtcgt gaccttgtca cacaagaaat cgggacccgg ctccgagcat
     3121 ccttccaacg cgtaacacgg tgccaatcct tctttttccg acaacgcgtt gcaatacgcc
     3181 agcgcttccc accagttcac attctccacc gggcatgatt cacagccttc aaaatacgat
     3241 ggttggtaac ccatgagcga ttgccattcg ccttgcgtta cttctgtttt cttcatcacg
     3301 aacgaacgtg tgagggtgac ctcatgggca gtctccgtat ccgctcgacc tggttcttgt
     3361 tcggggcttc ccatgatgaa tacggctggt gacaccaaca catagccctc tggtacgaca
     3421 agcccggcct cgttttcgac atcggcagac accgtatccg tcgcggcgtc ggcagaatcc
     3481 tcccctacgg cgtctgaacc tgcatcgaag tcagaatgag aatcggaagc accagcctct
     3541 tgcggcgtgg ggttttcttc cgttgtttca caagcagaca aagcaagagc aaacgacaaa
     3601 ccgatcatta cgtttctgag catggctgca cttccttgtt gacagatgca agcatgccag
     3661 aggtcgtagc ccgacggaag gagtcatggc atttgcacct gtcaatccgc tatgaggaac
     3721 caattgcatg aaagtcgcgg ataggtatag tcatcttctt ttccgatttc tgaatgttga
     3781 tgagaccgca tgacttctga cgtgaccctt tttgcctccc atcttgggga tcctgaagct
     3841 acgcaggctg ttccgaaaga agaattgacc ctgctacggc gtgttctcca ccatctcgag
     3901 gagcgtcgag ccgcaatccc ggctcttcaa acctacgacg ctgaactcat atcgcttcgt
     3961 gatcaggtat ccgaggcaag gctcgaagac gtgcctgcgt tactcgccca gatggagcgg
     4021 cttgcgggca tcagcgcgca acgtaatgaa ttgcgcgtca atctcgtcga cccgggcaac
     4081 ccctacttcg cgcaccttgc catgcgagag caggacagaa gtggtacgcg cgatcgcgaa
     4141 gtgctcatcg gacgaaccac ctacatcgat gctgcttcca atattcggat cgtcgattgg
     4201 cgtcacgcgc ccgtgagcca aatttattat cgttatgcgg aaggtgatgt gtatgaggaa
     4261 cgattcggcg atcgagaagt ggaaggtgag cttctgattc gcaggactgt aacgatacat
     4321 aagggctctt tggttcgaat cgcgacaccg caaggaacgt atgtgcgcca acaaggcgac
     4381 gattttcggt tgataccccc gcgagccgcg gacttgtcgg ggggacaggg aaaggcgaca
     4441 cgtccggcag aagccaagtc acgcggaaag ctcggtgtcg ggcccgatgg acaacagcgt
     4501 atcgatcggc acttgcccga gatctcagcc ctgctcgacc ctcgccaatt cgacctcatc
     4561 tctcgtacgg attccggcat cgttgtgatt caagggggcg ctggaagtgg gaaaaccacc
     4621 atcggattgc accgtctcgc ctttctgaat tatcaaggtc caaaaaagtt cgcaggcgag
     4681 cgcatgatgg taattaccac cagcaaaggg ctcgtctcct acacgtccga ggtcctgccc
     4741 gccctcggcg ttcgcgatgt gcaagtccat acttttccca cgtgggcgca acgcatgcgc
     4801 cgcatccact tcccttggat gtcggatgta gaagtgacgg aagacacgcc cttgtccgtc
     4861 tcgaaactaa aaaaacaccc cggaatcctt cgcgcgatcg agcgtcgatc acaacttgct
     4921 caagaagacc ccaaactgcg ttcggacccc catgcaccag tcactttgtg ggccgaattg
     4981 ctcacggatc tcgatgcggt acggcaagcc atgttgctgt ccgatgctcc ggctctcgag
     5041 gatgccgaaa ttcagcaagc ggttcgttgg tgttccgatc gctgcccgct cgtggctgag
     5101 ctgcggcctg gagagcctcg cgcagagcct gctgcccctc ctgatgaaga ggatgacctt
     5161 cgaatggatg tgggcatcga tgggcttccc accacggagg accatctaac cctcgacctc
     5221 gaagacgatg ctttgctgct tcgtgcctat caaatcttgc gaggccccct gcgaaaagga
     5281 aaagaaccaa ttcaggtcga gcatttgttc gtcgatgaag cccaagactt tgccgccgcg
     5341 gaattggcgg tgctcatcga tgtgaccacg gaacgtcgaa gtatcacgct cgccggcgat
     5401 acatcccaac gtctcgtcat ggataaccat ttccgagggt gggaagggat gtttcgcgat
     5461 ctgggcatcg aggcgattgc gatcgagccg ctcaagattg cctatcgttc tacccgggag
     5521 atactcgagc ttgcgaaaga cgtactcggt ccgatggcgg atgctcttcc ttccgtggca
     5581 ccgcgcacgg gtgctccggt cgagatgttc atcacgccgg gacctggtgt agcggttgcg
     5641 caacttggag aagccttacg cgcgctcgcg cttcgggagc ctcgggcgac ggtggctgtc
     5701 ttggcccggt attcggacca ggccgatgtg tattatgatg ggttgaggcg tgcggaagtg
     5761 cccaacctgc atcgggtcaa agagcaggat tttcgttttc gccctggggt ggatgtcacc
     5821 gaaattcggg aagtcaaagg gctggaatat gactacgtca ttttggtgga cgtgaatgcc
     5881 tcgacatttc ccgatgatat cgagtcacgg cacttgttgc acgtggctgc cacccgcgcg
     5941 gctcatcagc tttggttggt ggcaactggt cctgagtcca ccgtacttcc ccattggttg
     6001 acggatacag ccctttgatt ctcgtacgga tcgaacggac ttgatcgctg gcgccacccg
     6061 gcgcataagg gacctcgcca tggccaccaa gcgccactac aaggattggt attcctacga
     6121 gactcgccaa acctcttggc tcgaggcttt ctggatgatc gtgttcacgg tcgtgctggt
     6181 catcggcttt ccgctgatgt ttgcggccat gttccgatac gccgtgcctt tgctttttgg
     6241 gttgctataa gcctgcgaaa taaaaagaaa tttggcaggc tggcgttgga attcccctgt
     6301 tgtccgacat ggccgatgca tccctcgaag ccatcatcat ttcatctctc gtactttgtt
     6361 tttgatttgc tggcccgcga cggtgaggtc ctcggcggct tctcgcgcgg gttgcgatga
     6421 aaaaatggcg ttaaggtgct ggtagggtgt tcgccttcca agcggcagaa aaaagaatgc
     6481 atagacggca agcgccagta tcaacagctt cagacccgca gcggcagcct ttcgcaacat
     6541 cgtgatcgga aacggtatca gagtggttgg cgaacggaga agtttcgggc ggctcagtcg
     6601 actcgaagat agccgttgtc gatgagtttg agtatggcct gggcagtatc cagatccgac
     6661 gaagggcttt gattcaggac ctgctcgaag taaccgaagt tatgaacgag ctgcagaata
     6721 tcgagttctt gcggcttgag atcgcgtaat gccggctgca gtggcatcgc catgtgcaaa
     6781 cggctcgtga gacgcggaag acgttccttg atctggttga gttcatccat ctgccgcatc
     6841 ccctccatca acacgccttg caccggcatg tcgaggggat tcgcgaaggt tcgctcatca
     6901 gggggctcca aatcgaataa gccattcgtc caagagagca tgcgaaacac actcttgaaa
     6961 ggatggattt catccatgtc attgatgctg gcgaaataca ccagaccctt gcgcatgtag
     7021 atgcgaccaa tgacatcctc ttcggttcga atgacgagca ccccgctttt tttggatgta
     7081 ccaagaagct gaagaagatc aggaagaggg atctcttcga tggaaccgga catgcttcgc
     7141 acctggctcg tgcgacgatg ggctgccacg atttccatgt ctcgccgcgc ctgctcttcg
     7201 gacgtacgac ggacggattc ggagatgacg accttgagga tggatgtacc aatgaggact
     7261 cggtctcctt cgcgaagctt cgagcgcttg attttctcac cgttgacgaa tgtaccgttc
     7321 gtggaagcaa gatcctcgat gaagatatcg tccccttgaa acgcaatacg cgcgtgcttg
     7381 cgggaaacca tgtcctcggc gagcaccata tcgaggtcat tggatctccc gatatgtatc
     7441 tccttgccgg gaaccagcgg aaactcgcca ccctggtact tgcccgaaat gaaccgcagc
     7501 gccagaggac ggttctgaga ggatccggga ggcgggggtg gccctttgga gactggattc
     7561 tgcatgatcg tgctgaccga agtcaggggt ctcgtgttga gggaaacttt agttcccgcg
     7621 ttggataacg gtcaagcctg cataggccac accatacacc gggttgttca aagatcgcga
     7681 attcctttcc cttgaagctc gatcgagccc ctgcttcccg cacaatccgg ctgaaaactc
     7741 gtcctacgaa actgccctcc cccaatccct gcccgggaac agcgacactc acggtagagt
     7801 cactagccgg tgataccccc cgcggcttcg gagggctcct ttcgaagaga agcgcaaggg
     7861 acactttcct gcgaacaggc aggcaacacc accttgaagg cacttcccac gccttgttgc
     7921 gatgcggctt cgatgacccc ccctgcctcc tgcgcaatcc tttgcgaaat ggcgagaccc
     7981 aacccagtgc catgcgcttt ggtagagaaa aagggaacga acaggctgtt caacatctcc
     8041 gacgaaatcc ctggccccgt atccttgatc acgatctcca cccaacccag gcttccaggt
     8101 tccccttccc gcattcgtgt cgatatggtg gcaacgccgc ctcgttcgcc catcgcctgc
     8161 atggcattct gaaccaaatt gatgatcacc tggcgtagct gctctgcatc gatccgaacc
     8221 ggcagcaaat ccggagcaac atcaacctcg agacgggttc gctcatggct cgaagggctc
     8281 aagatctgca ccgtccgttt gacaaccgcg ttcacatcca ccggtgccgg attgcctttg
     8341 cgaggtcggg catagtcgag aaccgagccg acgacccggt ccaatcgatc cacttcttcg
     8401 atgatgatat cgaggaagtc ttgcgaatgg acaggaccct cttggtcctc gaccgagtcg
     8461 atgagaagtt gtgccgcccc tttgatggaa ccgagtggat tttttatctc atgcgcaagc
     8521 cccgccgcca tttgtcccaa caccgccagt cgatcgcgtt ccttcatgcg tgcatacgct
     8581 cgcgtgttct ccaccacgac tccgatcatc gacgccaacc tttcgagcaa tacgatctcg
     8641 tcgcggctaa aagcatcgcg aacacgttcg tctcgaacga ccaatagacc gatcacatcg
     8701 ccggatcctg gccgaatccc aaggatcaca ccgctgttgg cgatgggtcc caccagtcgc
     8761 gcggccgtca gaagagcatc gcgccctgtc aaattgtccc cgtgaaaacc aaggctccac
     8821 gatgatagcg cgtagtcatc gataaccacg gagtccgctt gtcttagcct ttcgagcaac
     8881 ggacgcgcgg tagctacctc gatacgttct tccgcgccaa gcccaaaacg atgcagcaag
     8941 gacaattccc gcccatccgg atcgagcaag tacaaggccg ccgaagtagc tcgacggctt
     9001 tcctcgagtg cgtggatgac gacgcttgcc atttcttcga gttcgaggat gtgaatcagg
     9061 cgcatctccg catcgcgcag cacccgttcc aattcgtgtc gctgacgaaa aaacacgacg
     9121 tggatctttt cttccacttt gtttcgaagt ggttcgaaca aaacgaggat ggcgatggct
     9181 gcgagaaccg cgttcaagta catcgtgtcg aagccgacgt acgtcacaaa cacatagaag
     9241 attccggcga gacagaacgc cagggctgtg gataccacga ggcgtccaac catttcatat
     9301 aggtcgatga gacgttcgcg ttgcatcgac tccgccaagg caaaaagaaa tacgacggaa
     9361 agcacggcgc ctacgggtgg aatggccgcg ccgatgaacc acacgaagtc tccaatgcta
     9421 aaaaacaacg cgaacgttcc caccagaacg agaagccgta cacgctcgcg taccgctcgg
     9481 gatggcgctt tcctgccctg catcgcaagc cccgccagcc ctgaagccac caatccgaat
     9541 acgtatagga atatcgtggt tcgaactgcg gcgtgggttt gatagtcact gatcgccagc
     9601 agcaacatcg gaatgccaag caccatccca acgcgcaata acttggagtg ttcgttggct
     9661 ggacgcggta cgatcgcttc gaatagatga agagcaaatt gcggtaacaa caccgcgagc
     9721 accgctgtga aacggtacca aatatcggct tgaaaatatc cgaacaacgc ttgggccaag
     9781 taccacagcg ctactgtccc agcgaaagcc gacagcaagt aatgcgtgcg gttctttcgc
     9841 gaccgaagca tgatcgatac ggtgatcgcg gtcgcaaaag ccgcacacaa catgatggtg
     9901 cgtgtgcgta aatccatcga acctcagcct aaaacctgtc caccctcgag tcgactcgct
     9961 tgccccatga cgccctcact cacgcaacga tgagtcctgg cggccaaggt cttagcggtc
    10021 ttctttacga cgaacccgca accctgtggc acgatcctcc accacccagc cgcgctcctg
    10081 caacaacgcg cgaatctcgt ctgctcttgc aaaatccttc ctgcttcgag cctcatcgcg
    10141 ctctttgagc aacgatgcaa tctcctgcgg aatcgcttgt tgaggctcca aggcaggcag
    10201 aatcgacagt acgcgatcta cttccgtgag gccctccagc accaacttcg cacatgcttc
    10261 gcaaagcttg ccctgaccca tcacgtgatt gacctgacga atcaaacgga aaatccccgc
    10321 aatcgctgcg ggcagattga catcatcgta cagcgacgcc cgaaatgtct ccttcatccc
    10381 aacgacccaa ccagccacct cttccacatg ctctccttcg gtgcgtacct cctgtaagtt
    10441 gctcacgaac tcatcgatac gacgcaaact cgcgcgcgcc tcctccaaac gttcatcggt
    10501 cagccatacc ggttgccgat aatggtgacg aagcaagaag aaccggattt ctcgcgccga
    10561 gtaccctcga tcgatcaggt cttgaagcgt cacacaaccc tcctcgtgtg tcatcttctt
    10621 accgttgacg agcaccaact cgctatgaag ccaaaattgc gcttgaggct tgcccgtgag
    10681 cgctcggatc tgcgcgatct cgttttcatt atgaggaaaa acgagttcga ccgacgccat
    10741 gtggatatcg aatcgatctc cgagtgtcgc gcgcgccatt gctgaacatt ggacatgcca
    10801 gctcggacgt acgttgcccc actcggtctt gtagctcact cccttgcgaa gctctcccag
    10861 ggtcgaacga cgaaacagcg taaaatcccg tggatcgttc ttgtcgtacc gctccaaatc
    10921 caccgtcgcc cccatccgaa tcttgcccaa atctttgccc gacaactccc catacgacgg
    10981 cacacgacca atgttgaaat acaccgagcg aagcttctca tacgccaccc ccgcgtccat
    11041 caactcgcgc gtcaacgcta tcatcgcatc gaccgactcc gatgcccgca cgtattgatc
    11101 cgccctcgat atcttgagcg catccaaatc ctcaaaaaaa ccctgctcat ggcgacggca
    11161 aagctctgcc atcggctctc ccgttcgctc cgactcttgc acggtattgt catccacatc
    11221 cgtcacactc acgacatgct gcacgcgata cccagcatgt tcgagcgtac gacgcacgag
    11281 atcatcgacc agcatgcgac gaagcaaccc gagatgaggt cgtcggtgaa cggtgggacc
    11341 acaggaatac atcgtcacat cgtcggagga agacagagga acgaaaggct cgtaacgccc
    11401 actgagcgtg ttgagaaacg acaggcgagc aggaacgaca tccggttggg caacttggaa
    11461 aagcgtggtg ctgaggtatc gctctccccc atcacataaa atggtaacga tgacgcccga
    11521 ctccatctgt ttggcaagtt cgacggcaac gtgaacgtcc gcaccagcgc tcatgcctac
    11581 gagcaaacct tcctcacggg cgagacgacg agccatttcg tatgcggcat catcgtcgac
    11641 attgattttt tcatcgaggg ccccggcgtt gtaaatgccc ggaacataag actcttttag
    11701 gtttttcagg ccctggatct tgtggccgag atagggctcg acgccaatga tgcggatttt
    11761 cggattgagc tgtcggaatc gacgtgagca acccatcagg gttccggtcg ttccgaccgc
    11821 cgcgacaaaa tgggtgatcc tcccgcctgt ttgctcccag atttccggtg ccgtactttc
    11881 ataatgagcg agggggttgg ctggattgtt gaattgatcc gcgagaaagt atcgatccgg
    11941 ctcttgggct gccaactcat aggccacttc gatcgcacca tccgtgcttt tttccgcggg
    12001 cgtgagcagc aactccgcgc cgtaagcggc caggattttt cttcgttcga ccgaggcgcc
    12061 ctcgctcatg gcaagcacca gacgatagcc cttcaccgcg gcaaccatcg ccagtccgat
    12121 accggtgttg ccggaggtag cctcgataat cgttttgccc ggatgcaatt caccagtctt
    12181 ttccgcctgt tcgatcatcc acagcgctgt gcggtctttg atggaacccc ccgggttgtt
    12241 gccttcgagc ttcacccaga tctcgacgtt cgggttggga ttgatcctgc gaagacgaag
    12301 caagggggta ttgccaatgc gcgcaagaac gccttcccga ggcggaatca tccgtggttg
    12361 cgtggtgtcg tcagtcatgg gacgtgttta tcacattcgg acgccacgat cgtgaaccgt
    12421 gcgttggctc gttcttcggc cacgatcttt tcatccaaag gactatcgaa tagccaaaac
    12481 accatctcct ccgcttccca cgtgcaacgt tcaccatggt ttcgcaatcg cccttcggcc
    12541 gcggcgcgag tcacctcgat caacggaata tcccgaagat cgcgctgcat cgcttcgaac
    12601 cttccctgcc gcctcgaatc cgcgaaaacg aagtggtatc tcccactata ggccgtgtga
    12661 taccacgcgg gccgatacgc taccccttcc aggcccaaac ggcgtgcaat gcgcgccaag
    12721 gcccatccaa tctcgcgagc aagacccagt ccaggaacct cttgaccagg aagttgcgga
    12781 cgcttctcac tgaaccgcgc ccgaggattg cacagcgcta gccaatgcac atacagcaca
    12841 tcccgctcga caacacgtcg acgctccaac atgcactcga ccaatacgtg ctgctttccc
    12901 aaggcattgc ccatcacacg aacccggtct cccatatcca ccacgtcgat tgccgcatgc
    12961 acctgttcga aaccgaggcg tcgtatatga ggaatgatgc cgaagtgaga cagggcgtat
    13021 tcaatccctt ccgcggtata caaaccgagc aaacgaggtt gttcgggagg gcgaagcccc
    13081 aaggcttcct ccaggtcttt ggccgacaac tcctctgcgc cttcgagtcg ttcacgactc
    13141 aacttggtgg agacccatcc ataacgagcg cgcatcgggt catagctggg tggaatggcg
    13201 gtcgatgacc gacaagtcag catcaacccc gtgcccgcaa gcactttcca cgattcccga
    13261 ccatatcccc ctccggccgt ccataccgat ggaacccggc caagcgcttc gagcaccatt
    13321 cgatcccttc gacgcgctcc ttcgagagtt agtgcgagct tacccaatcg gtctcccgcg
    13381 accacgtctc cccccgcgat cacgaatgcg aagtcagcac ggggcatgcg gctgagcata
    13441 cgatccaacg cacataaata cgttcgatcc tccgagccgc gaggcaatac cgtttcgtcc
    13501 accccctcga gcggacccca actttctccc gaaatggacc ccacccaaac ggaagaatct
    13561 ccacgaaaac acgctgccag accatccggt ggatgcgcgt ctacatcgat gaccacgatc
    13621 cgaccacgga atccttcgtt tcgaagaact gcagtggcca ccgcaatatc gttgaccgca
    13681 caaaaaccac ccgcagaatc cggcgccgca tggtgaaatc ccccaaataa attcagggca
    13741 attccaccat ggacaagtcg acttcgcgcc gcttgtaccg tcgcaccaca cgcgaatcga
    13801 atcgttttca gaacctcatc aacctgcaac tccgtttcat ccgcggcaaa tattcgtgcc
    13861 aacgttcggg gctgactcaa cgattccaag tactcctgcg tatgaacacg cgccaaatcc
    13921 cgataacgaa cccgattggg tcgctcgagc attccttcct cgatcgccca cgcgtcggcc
    13981 aaaaaccaaa gcaccagatc cgctcgacgc ggctcgaatc ccgtcaaccc tcccacggca
    14041 ctcaagggaa gacgaaatga acgatgatgc catacctccg tgtggcgtcc caccacgtga
    14101 cgccggagta agttccgaaa acgaagctgt ttcataaggt attgtcgata aagggagaag
    14161 cgacgatatc acccaacggc ttttccaacc cccatggtcg cccaagtccc ccgtcaaaaa
    14221 aagccattca actccccgaa tcgccacggg tgccgatctt ttgccacgaa aacacgaaat
    14281 agttacttgg atgaaatcgg gtagacatcg gtgcagaacg atactacatg cacacaccag
    14341 gtgttttgat ccggagaaga aagcgtgaat aaatatctaa gtattttcat gtacttatcc
    14401 cttggttctt gccttgtcgg ctgcgcgctg gacaccgttg cggacgagtt atcctccgag
    14461 caggtcgatg ctacggaagc ggatctgtcc accaacgccg tgacattcgt taccgtgcgt
    14521 aaggacaacc gaaaatgtgc ggcgccctca tgcggcggat attgggtcaa agacgtcaac
    14581 cgatcgtctg gtgagcaata tgtctccgga ttcgatttcg gttcatcggg attcgacgaa
    14641 caactcatcg gcaaggtgac cgacgcgccc gcggatgaac tcatccttcg cggcagattg
    14701 ggaaaaaaag aagccaagca cggaacgcgg aaattgttgg tgtaccaggc gtttcgaggt
    14761 cttcccggcg tcaaaccgct gcccggggac gtgtatttcc aagccgaaac acgcgatgtg
    14821 ccgattcaat gcttcaccgc accgtgtcct aacgagatcg cgcatcggct caacaccaat
    14881 caacaatacg catttagcgg ttatcaggta gccttggccg cgcgtcccca cgtggatcaa
    14941 gattggctgg tcgaccgcat ccaacaccat aaggcggtcg ttgcggcgtt attcgtaaaa
    15001 ggcgacaaat acccaggcgg ctatgaatac cttctcgatg ccagccaagt ctatctcgac
    15061 atcgccgatg cggtgggtcc ctgtccggcc tataagcttg ccgcttgttc ggaacccgac
    15121 gccgattggg cctacacgcg cgacgtaaac ctttgcttgt tgccggaagt ttgcatgagc
    15181 accctcggtt gcccgtcgct tcagcctccc cgctgtgaag atggctacag cgtttccgca
    15241 tggcgcacga actcaccgag ctgcattcgt ttcgcctgcg atccctcctt cgttcttccg
    15301 aattgagcgt ccttatctac ctgaatccga atggaatccg agaaggacgt tggtgtctcg
    15361 tcggagggcc atggccgaat gggaatggtt cgacggcgtg ccatcaccca tcgatagggt
    15421 ggaacttggg ggatggtatt cggaaaacgg ggattatttt ttcgcggtaa gggcgtacgt
    15481 atcctgagcg atcatcaact cttcattcgt ggggatgatg aggatacgaa tgggggaatc
    15541 ggccgtggcg atgtcgagtt cttttccgcg aacaccgcga tttttttcca cgtcgatttt
    15601 gtaaccgatg ttttggaggt tggaacacac gcgttcgcgc acgacatcgc tgttttcgcc
    15661 aacgccggcg gtgaagacaa gcgcgtacat tgccgatggc agccgtgtag gcgccaacat
    15721 attttttgat gcggtagcag tacatttcga gggcgagggc cgctcgcgta ttgccttcgt
    15781 tggctgcttg ttcgaccgag cgcatgtcgt tggacagacc cgagacaccg agcatgccgc
    15841 tctttttgtt gagcaggttg tcgatttgtg cgacgggcat gtctttgaca cgggcgagaa
    15901 aagcgatgat ggcggggtcg atatcgccgc atcgggtgcc catgatgagc ccttccaggg
    15961 gggtaagccc catggtggta tcgatggatt tgccgttttg gatggccgtc atgctcgcgc
    16021 cgtttccgag atgcgcggtg atgagattcg tttcctctag tttacgtccg agaattttcg
    16081 cggcccgggc agccacatag cgatgcgacg ttccgtgaaa gccgtatcgt cgaacgcgaa
    16141 gttgttcgta cagctcataa gggatggggt aggtgaaagc gtaggccggc atggtctggt
    16201 gataggccgt atcgaataca gcgacctgcg gcacgttcgg caacagttcc cgggcgactt
    16261 tgataccgag caggttggga ggattgtgca atggggcgag ctgaatatgc tcctccacgg
    16321 ccttttgcac cttgtcatcg atgattacgg tttcggaaaa agcttctccc ccatgcacca
    16381 cgcgatggcc cacggcgccg atttcggaga tatccttgag gccgcccttg gactgatcgg
    16441 taagagcatc gatgatgagc cgcaaacctt gaccgtggtc cgccactttt tggtcgatgg
    16501 tgtatttgct atcgccgatg gtgtaggtga gcttgctctg ttcttcaccg attttttcga
    16561 gcaacccccg agcctgtcgt ttttcggagg gcatttcaaa aagctcgaat ttaatcgatg
    16621 agcttccaga gttgagaacg agaaccttga gcgacatggt gggttagctc ctctgagcct
    16681 gaatggccgt gatggcaacc gtgttgatga tgtcggggat ggagcagccg cggctcaggt
    16741 cgttgacggg tttgcggagc ccttgcatga ccgggcctat ggcaatggct ttcgctgccc
    16801 gctgtacggc tttgtacgta ttgtttccgg agttcagatc cgggaaaatg aacacggtgg
    16861 cagctcccgc gacgcggctc cccggaagct tcgcctttgc gaccgtcgga tcaatcgcgg
    16921 cgtcgtattg gatgggtcct tccacgagca agtccggatt gcgctctttg acgatcgccg
    16981 tggcttcacg cactttgtcc acatccgaac cgctgcccga cgaacccgtg gaatacgaaa
    17041 gcatggccac atacggttcg atatcgaaag attgtgctgt ctcggcgctg gcgatggcga
    17101 tgtccgcaag ctgctgcgcg gtcggattcg gattcacggc gcaatcgcca tacacgagca
    17161 cttgttcggg taaacacatg aaaaacacgc tcgaagcgat ggagtagcct tctttggttt
    17221 tgacgaactc gagtgccgga cgaagggtgg ccgcggtcgt cgtgatggaa cccgatacca
    17281 tgccatccgc gagcccttcc tgaaccatca tcgtactgaa gtacgtcgta tcgttcatga
    17341 ggtcgtaaga aagctcccac gttggtgttt tctttggcga gcgcaattcg atgtaccgct
    17401 tggcgaatcg ttcacgcagt tcagatgtgg ctggattgat gatttgcaga tcgccgttga
    17461 acttgagccc ctggcgagaa atctcacgtt gaatctcatc ttcgttgcca aggatcgtga
    17521 gattgacgat gcctcgctcg agcagaactt gcgctgcacg taagatacga ggttcgtgtc
    17581 cttccggcag gacgatgtgt tttttgtctc gacgagcaat atccatcatg cggtggatga
    17641 attgcttggg tgtcacccga cgggatcgag gttgcacgga ggctcgtgcg atcaaaggct
    17701 ccacatcgat attggattcg acgagggttt tgacgagttc gacgcgcatt ttttggtgag
    17761 cttgcaagct cggcttgacc tgattgacca agatggcggt ttcgtaggtg ccggtctgca
    17821 ctttgagcag tggcatgtgc ccacccgtca ggtcgagcac cacgtgtttg acgtttttgc
    17881 ccggttcgag cccgccggtc atgatgatcc ccgcgggtgc cggaatcgcg ggagaggcaa
    17941 acgcccccgc cagacccaga agcacatcat cacgatcgcc cggcaccacc accagggttc
    18001 cccgcggaat ccgctcgaga acgttctcta cactcatggc ggcaaccgcg acctcttgca
    18061 cgattacgtt gagccggtct tgaccagcta tgacttcggc attgagggca gccgcaacct
    18121 cctcgattcg cagcttgctc agcatgtccg tgcgaggaac cgtgcccaat agcttgagcc
    18181 ccatcttcct gagcgacggc tccgcggcat catgaatggt cttgacgtac ttcggatccg
    18241 cgcgattgat gaccgctccg aacatctcac atcccttggc gtcgaaactc tccttcacca
    18301 tcgtgatgtt ttggatcatc tgatcgcacg gaccgcactc acaatcatcg gtaagaccca
    18361 cgcaattgtc accgttggcc acgagaagaa caggggatcc aaggtttttc gagatatccg
    18421 cgttgatgtc gaattcgaac gcggacatcg cgccaaagta gtccgtcccc tcgcaaacca
    18481 cgaaatcgca cttcgaagcg atcttctcgt acgcctccaa aatcgtatcc aacaaatgat
    18541 cgtaggtgcc gttgctcaac gcttcctgca cctgatccat cgtcacaggc accatctcct
    18601 cgagcgaaaa ctcgagcccc aacgcatctt tcatcaaacg tacgtcagga tccgtcccat
    18661 ccattccccg ctgcccaatc ggtttgaagt agccaacgcg agaaagtgtc gactcgagca
    18721 gatgaaccaa tcccagtgca agagtgctct tgccactgcc gcgctgggtt gccgtaacga
    18781 gaatgctgct tgccatgtgt ctctcctaag cgatggtcag aggatgcgca cgaaccggtt
    18841 gagcctctca ccctcagatt gaattcgcga tgaatccgtc acaggaaaga ggttctcatc
    18901 aagaacgtga cgtggtcccg cgcttgcacg aaaacaaggc tttttgtgga ttcggaacca
    18961 acacggagcc catcgctcca cccgctccca tagccgcaca cccaagacca gtcaaccgaa
    19021 cgacgacacc ggcacccaag aaccgcttcc gtggcttctc acttgccttg tcgtactttg
    19081 tctcgcgaat tgcttggtgg ttaggcacga tatgtgtccg tgttctctgc ctcgtgttac
    19141 gcaccatctg ccgtttcacc gttttccctt ggcttgacaa ttgtcacgac gcgacggcat
    19201 gcgtgatgtc gagcttgcct gatgccacaa ccggagaaaa attctgcgtc aattcgttgt
    19261 aatctattgt tttttcttgt gattattctg gtcctctggg tgtcgaccat ggcgggagag
    19321 cgcggacatg aaatcgccgc gcccgcctct cctccctatc tgactgtctt cttactcgat
    19381 ggtgcccgcc acgacgtcgt gtttcgcgag cttcgctccg gaaggctccc ccatatccaa
    19441 cagctcatcg aacaaggtgc cctcgtggaa aacggtgtca cctcttttcc aagcatgacg
    19501 ggctacgcct attatcccct cgtgaccgga cacgattcgt cgcgcagtgg cgttcttggc
    19561 ctgcgctggt tcgatcgttc cagggatttc ggaaaccttc gaagctacgt cggcacgacc
    19621 aacgatcaaa tgtccgcgga cttcgcgccc cgtcctcgct tgctttacga acgtttcccc
    19681 catcagcatt cctactcgtt caacacgtac gccaaccgtg gcgtcaaaaa aaacgtcaaa
    19741 gcgggctttg cctacgcacg cgccaagtac catcgccgtt ttgccgttct gtccgccctt
    19801 ggccgtttgc ccatgcttgg aaaattcttg atgcccgatt gggaagaggc tgaagctcag
    19861 gggctcgaaa ccgctctacg ggatctaaaa aaatatccaa aaattcaatg ggttacgatt
    19921 gcgtcgatgg atgcccacca ccatgtggcg ggcaccgatg accgctatgc ggaactcatt
    19981 cgttatgcgg acgttctgat tggacggtat cgggatacga gtcgggcttt ggggcaagag
    20041 acggaccgca tctatctgat cacgaccgac cacggcgcgg cggacgcttc tgacaacttg
    20101 gatgtgcgag aggtgctggc aagttgttgt gggttgaaag cacagcgaga ccgttcgacc
    20161 catctgatgg gggccgcgct tgatcagcct ctgtcggatt ggtttggcaa tgacgcggtg
    20221 gtggtcatca acggagatat ggtcaactat ctatatttca gcgatcctga gcgctccggc
    20281 gcggaatctt ggcgtcatcc gatgaaatgg gaccgccttc gctcgttcaa aggcaaggat
    20341 gtggtggtgt cgttgagcca gcagccaggg atttctttgg tgcttggatg gagcgacgaa
    20401 cagcaggccg cgaaagtatt ttcctctcgc ggagaagcta cgattcgcac gcaacccgaa
    20461 gggttgtcct attcctggca ggggcaggat ccgttggaat acgaggcagt agctcgcgtt
    20521 cgtccgttgc tcgatggcaa acctcacgat gaccgagcct ggctcgatgc cacttatgga
    20581 actcgttttc cggacgcggt atttcgaatc gttcggctgc ttacggcacc tggcgcgccg
    20641 gatttggtgt tgacggcggc gcaggggtgg gacttgggaa aagattatga agtgctggtg
    20701 ggcaactatc gtgggggcca tgggggcctg cgagccgagg aaatgctgac atggttcgtt
    20761 cttgccgggc cgggaatccg taagggcgta cgattggcga cagctcgcac ggaagacgta
    20821 ggagccactc tttttccgtt gcttggaatc ctgccggacc cggaagccac aggaaaaatc
    20881 ttggaagagg tgctcgagcg atgactgatc ccactacaac tccgcgcacg atcagcgcgc
    20941 ggctcatcgc caaactcgaa tccgggctgg cttttctgtc attgctatgt tttcgccgtc
    21001 cctggattgc gctgggaatc ctcgctctgc tgacggtcgg ggcggcccag gccgcacgac
    21061 agcggctctc gctcaatgcg gatctggccg agctgcttcc ttcctctttc gaaagcgtca
    21121 aggatctcga aacgctcaag caacgatatg gcggcatcgg atacgtcggc gtcgtagcga
    21181 gccatgccga acccgatgtg cttcggagat ttgtcgacga cgtagcgcca cgagtcgacg
    21241 ccctcgcgtc cgtgagatcc gtagactatc ggaaacccac gcaatttcta cgtgatcacg
    21301 ccctttatta cctggatctc gacgacttga aaatcgtcca caatcgattg cgcacacgct
    21361 tcgacttcga ggtcgccaaa cgaaacccca tgatgctcga cttcgatgac gaagagcccc
    21421 cctccgtcga cctatccgat atcgaaaaaa aatacgaagc acgcagtgat caggcttgga
    21481 tgcgaacgca gctcgacgat gagtactaca tcgacgtgga gaagcgtcga attgccctgt
    21541 tggccaagcc gtcgaaaatg agcgtggatc tggcgttttc ccggcagatc gtgggcgaga
    21601 tccagcaggt gatccaacag tcggaaccca ctcattacga tccaaaccta caggtgcagt
    21661 acgggggcaa ttttacgaag aaggtcgatc aacaggatat gatcgaaaag gatctgaata
    21721 cgggaaccat cgtggcgttg gtgctgatgg tggcctattt gctgctgcat tttcgtcggg
    21781 tgggtgcggt ggcgctcatc atgattccct tggtgatggg attgtcctgg gtatttggct
    21841 ttgctgcgat cgtgtatgcg cagctcaaca ttctgacggg attcatcggc gcgattcttt
    21901 tgggtcttgg ggtggatcac ggaatttatt tgctcggtcg atttcgtgag caatggcagg
    21961 gacgggacgc tgcggaggat gcgatacgca ttgcatttgg caatacgggt cgtgccgtgc
    22021 tcattgctgc gttcacgacg gccgttggtt tcgcggggct cggcatgtcg gaattccgag
    22081 cctttcgtga gttcggggtc atcgccgctt tcggcttgtt gcttgtggtg gtggcttatt
    22141 tgacgtgcat gcccgcgctg ttggcagtgg cggtgcgaat ggggtggcaa ccgcgcagtg
    22201 ccaaagcggt ccaacaatcc ttgtacgcgc gtctgcttgc gaagcgaccg gggttattgc
    22261 tcacggtctt cacggcgttt ttggttttca gtatgccgtg ggtcagcaag ctcgagttca
    22321 acacgaattt tcgagccctt acagccgcga atttgccttc ttttcagctc gattccgtca
    22381 tcgatggatt gctcggacac tctcaaaccc ccgtggtcgt attgaccgaa aaccgcgaac
    22441 aaagccgttt tgttgccaca acgttacgcg aacgaaaggc cgcttccgat tcttccacca
    22501 ttcatctggt ggcaaccggc gatgacatcc ttcccgatca gcagatggag aaacaaacga
    22561 tcattcagga tatgcgcaag atgatcgacc gattggcttc ggagaagatg gggcccgacg
    22621 atcgaagaaa agtggatcgg atgaagcgac tggtggccgc ggagcctttt gatcgtcagc
    22681 aacttcccga tgctcttggg cgcatgtttt tcccggatcg gaaggacgtg ggggattttg
    22741 tattggtcta ttcttcggtg gacttgagtg acggcgccaa cgtgagtcgt ttcgcgaaag
    22801 aagttcgggg gatcgaattg ccgcagggag ggcatgcttc cgctgcggga gaaccaatga
    22861 ttctggcgga tattttcgac ttggtcatgc aagagggacc accggtgttg atcggaacgg
    22921 tgctgctcat ctttttgacg atgtgggtat tgatggggaa gttttggcgt gcgtgcatgg
    22981 ctttgtttcc tgccatggtg agcttggtgg ccaccttcgg attgcttggc ttgctgcgaa
    23041 ttgaactcaa ctacttgaat atcgtgttga ttccggtgtt ggtgggaacc ggggtcgatg
    23101 ggggggttca tatgctcacc cgcggggcga gcctcgatct cgtcaatgcc gcggatcgcg
    23161 cttctgttcc tatcttcggc gcgttggcca ccacggccct tggttttgga accttgttga
    23221 tggcacatca tcttggtctc aactccctcg gttccctcgc tgtcgtggga ttggctgcca
    23281 atcttttcgc gagtctcgtg ggtttgccca gcctgctgct cgtggtgcaa cgctggcgcg
    23341 caactcgcgc atgaatctca ctcgattcgg tgacggttgc atcttatagg cccaagggtt
    23401 agtcaacgga acgtgacgga atccaaacgt taatcatgtc gttggagatt tcctggtggt
    23461 tactcgctct catcgtggga ggttcggctc cgctttggat tcgtgggctc acccgtcggt
    23521 gggagcaacg atcccagcag cgcacggaac ggattctcga acgactcaaa caactccagc
    23581 cgtcatcgaa tacgtcgcca acagcagaaa tagaccgaaa cgaatcatga ggcgaagctt
    23641 cgcttgcggt caacgcccgc gcgatgcatt cctagggtca gatcctgtca tgcatcaccg
    23701 tacacacccg atatcagcca acgaactgac cgatgctggg ctcgccgctc gatctcgggt
    23761 tccttcgtac gccaagcgga tgcctgactc gcacaccgcg acttcgagaa acaacgacaa
    23821 cgatcagcga atcgccgaag gatagcatgg aacgtctgtt cggattcatt ggtaaccgct
    23881 cggatcttgg ggctcgagtc ctggatgtag atgccccggc gctcgccacg tccgccgaag
    23941 gacgccgact ggggtggggc gttggattct accaagcagg ggaggttctc ctgcgtcgac
    24001 gccccatgga tgaccgccaa gtcattgctg ccgcttccct cacgcgtgac ttgagaacgg
    24061 atgctcttct tggaagtgtt cgagcgccgt ctttcggtga gccacgcaca caaaatacgc
    24121 agccctttcg atatcgcgat tggttgttcg tccatcatgg caccgtcaat gccttcgtgc
    24181 ggctgcgcga gcgattgatg gagtccattc ccgactttct tcgtggcaac attcggggtg
    24241 aaaccgatag cgaattggtg ttttatctat tcttgtcttt tctgcatgac gcgggaaaac
    24301 tgcgcggtcc cacggacccg gcggtcatga ccgaggcgct tcgtggcacc tgcacgttgc
    24361 tcgatcgcct ggcgatggaa gaaggcgccg acgacgcggg tgtcatgggc attctcgtgt
    24421 gcgacggcga acatatcgcg ggattgaatc gcaacgctca tctggcgttt cgtttggttc
    24481 acggcgaaaa cgatatcgat gccttgcttc ccgaagatgc gttgcggaat cgtcgattgg
    24541 tcgacatggc gcgagttcgt ttcaccatca tcgccagtgg attgacagaa ccccgcgtgg
    24601 gttggtcact gcttccgact ccttgtacca tcacgacgag tcgtctcgac gagcccatgg
    24661 tggaatcttt ctgatgggtt cttcggcttt tcgatcgaag catacgatac cgctgatggc
    24721 agcagcggtg atggtcatgc ccgcttgtgg cgccgtggca gtcagttcct tcgaaaccgc
    24781 cgccgcatta ccaaagagcc ctcgtcgtcc ggatgccgtg gccatcgatc gacaaagccc
    24841 ttcgcccccg attcaatcca cggccgacac ccgcgatggg ctcgttaccc tgcaagaacc
    24901 ggtcgatatc acgaacgcga tgcaaaccgt tcgcatcttc tttcgcgcgg ttcttcatga
    24961 ggacctggaa gcgatgtctt cggttctcac aagcgatgcc cagcagtatt catcggcctc
    25021 gggaaatgga atgcccatga atcggcattg ggaacgccgc tttcaaaaat tcgactatac
    25081 cgcggccggg cagcttccgt tccgcgagag catgacgcaa gtctatcggt atcaggactt
    25141 gatggtgccc ttgccgggaa gaccttcacg ccttgaaaac atgacttcgc aagacatcct
    25201 gattcgtgtg cccatcacgc gcactcgatt cggactcgac cgcgtcttcg gttccgaaat
    25261 gtattttcta ttgcagcggc agggacgaga gttcctcatt cgcaccctca tcgaagactt
    25321 cgtcgtaccc tgatgctctc gctcaaggca tcgtacacac gagatttctc gagccatcgc
    25381 tctgatggaa tgcgtcgaag gcaactttgc ctgcctttgc catggtgact gtcagcttcg
    25441 catacgttcc atcgtccaac ttcaacacgt aaatattctc ggacatcgta taacccgttt
    25501 cgccagcgcc tccgcttcgc cacgtgtctc cgatgagatc cggatccccg tcgaagcccg
    25561 cgtcgggtgc ttcgataaca tccaaaaagc tatcgccgtt tccaagattg atggcctcga
    25621 cgcccgcgcc caacgtgatc cacggcccct taccgctttt ggccaacttg aaatcccatg
    25681 cgctgggctc ttgtacgtgg gattccgtgt ccgacaatgt gaagtaccac gtgtccgcca
    25741 catgcaaatc gaccgcaaac ggttcacaaa gggccgtagc accgccagag cccgcggaac
    25801 cagcgtttcc tgccgcgccc gcttcacccg ctgagccccc cgtcgccccc cccccccccc
    25861 cccccccccc cccccccccc cccccccccc cccccccc
//
LOCUS       DAOSOR010000010        25608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_1922_length_25608_cov_4.511721, whole genome shotgun sequence.
ACCESSION   DAOSOR010000010 DAOSOR010000000
VERSION     DAOSOR010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 25608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25608
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1922_length_25608_cov_4.511721"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2069)
                     /locus_tag="PK710_01085"
     CDS             complement(<1..2069)
                     /locus_tag="PK710_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08324.1"
                     /translation="MRCRALRILFAHSIALWILLSSSVLWAGDPYLEWWTLHTPHARI
                     HYPKGLEPIAERVANWYEGLHEEMGEGLRWSPSHLVQIVITDDTDSANGSATAFPYNT
                     VRLFVTAPDDMSPLGDYDDWYLELLSHEYTHILQMDNITGVPALINAVVGRTFAPNQA
                     QPRWILEGFAIIHESRHSTGGRIRSTMFDMFLRADVLDERLMGLDQMSHSPRRWPQGN
                     VWYLYGSRFLSWIQDIYGYDVFAAVAADTGSQLIPYGLNRNIRRVTGRTYEELFVAWQ
                     QYLREHYRKQLDPVYRRGLREGTRLTTRGEWISNPRFVPPAARTQAEHTEVIFHGGNS
                     HDRSGFYRVLMDGSRRAQPLEEPLIVRADGQCSASFLPDGTMIYAALEPWRREYFFND
                     LHRIGPHQRAPSGTEPSIQRLTFGHRARDPDVSPDGRQIVYVVNTRGTSYLKIADISS
                     EGAIRDSRTLVPSARFEQAYTPRFSPDGKHVAYSVWTRGGYRDIRIVEVATGQFQQLQ
                     HDRAMDMQPSYSRDGKYLLFTSDRTGIANIYALELATGALHQVTNVRTGAYQPELSPD
                     GTTLVYVGYTSFGYDLFAMPFEASRFLEPLPYVPERPQAPVEPSRTNWERRPYNPLST
                     LWPRAITLSYGPGTYGQALTVQTTGSDVAGHHSIALSANFETERYDVSVEVYENKKEE
                     QRRSDDE"
     gene            complement(2117..3166)
                     /locus_tag="PK710_01090"
     CDS             complement(2117..3166)
                     /locus_tag="PK710_01090"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOT08325.1"
                     /translation="MSDIVSNARSARESLAKGLNLLQSNPTAPPEYLQAAEPIAKAMG
                     ALHRIETMGGPVLKTSATEALEAVRSALSILQSKAAMDPQMMQTMEAVASSLGTIFGL
                     TKSAEAEAAPSPQAPVHQAPPQPVVAQPPMHQAAPYQPPPQPPAFQPPPQAPYQPPVP
                     QAPPYQPPPQPAFFQPPPQVPQFQAPSAPMAAVGPANASPKVAAAPMPAAASSWSEAD
                     PFAAKPAVPTAEPYRGAANLPSIEAELGTHSATNFYRGLSGNDVVDHGGLFIATYNVP
                     AVGQTLKVQVSLPGGYEFEAIGIVRWTRESRESMTSDATSPGCGIQFTHISTEARQLV
                     YRYVRNREPLFHDDL"
     gene            complement(3193..4074)
                     /locus_tag="PK710_01095"
     CDS             complement(3193..4074)
                     /locus_tag="PK710_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOT08326.1"
                     /translation="MRIAFLGLPVAALLLLEDGHELVLAGLSGRWRTGERRLRARLGA
                     ERVVLDPHHHWAGYLAQLERLQPDLLVSWYFTRRIPMQVVHACKGGGIGVHPSLLPRH
                     RGPDPFFAAIDAGDDVTGVTVHRIEEDYDTGAILAQRRLPIDPSWNAWDLAKKLDRPS
                     LSLLRQTVAALDRGEPIDSVAQEESKATLAPFPTEEDRIVKWKWTAHTIVRRVRALAP
                     NPGAIVSVGEMPLVLLKADTRPCFAVLRPGEAAVIGGVAVVRALDEGVGLLQGEFEGH
                     RWQASDIAHQIASLIAK"
     gene            complement(4075..4347)
                     /locus_tag="PK710_01100"
     CDS             complement(4075..4347)
                     /locus_tag="PK710_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOT08327.1"
                     /translation="MQILVERNPSDSRLQALQVTSWPQWTKEPSTFPWTYEEQEICYF
                     LEGQVVVTPQGGTPVTMGQGDLVTFPAGMQCVWEVRRAVRKHYRFG"
     gene            4463..5224
                     /locus_tag="PK710_01105"
     CDS             4463..5224
                     /locus_tag="PK710_01105"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOT08328.1"
                     /translation="MTKDANKQTSPAPELTSALEDYLETIYELVRDQKLARVKDIAKA
                     RGVRAGSVSPAMRRLAAMGLIDYAHREYIDLTPAGERLARRIYARHELLTQFFEKILQ
                     MPSDEARANACSMEHSLSPQGMEHLARFFEFLQICPDGPELLAKFHACSVVHEGSPDC
                     LGSCQKVRNQQKNSQERLFSLWDVPHERQARVAQVEASAQVRQTLLDRGFLPGTRVVV
                     IRRSADGQSVWVQIGGFELQLEKNYAQNVLMILED"
     gene            5360..6778
                     /locus_tag="PK710_01110"
     CDS             5360..6778
                     /locus_tag="PK710_01110"
                     /inference="COORDINATES: protein motif:HMM:NF039689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6209 family protein"
                     /protein_id="HOT08329.1"
                     /translation="MNRIIAFLPLVAIALSSCSAPVDDADSPASVSAAQVPAPLVQKA
                     DSQDVADHGCLVVLRQVQRSPGPTGYEIQCTSERCWFVWSGTIDVARSLVEQGATVGL
                     MYQVGMGEWWEVPAEATESDDGPMQSFAFRFFDHAVDTSLSLTALAQARVQVIPVVHL
                     PGGGRLFDHNRVSSDLDNYVLNYGNDWSVNDDDSVCTVGAPTASTLEFLAGWKHVQHG
                     TIVEGGTLTIQYDLARLPDCRGTHNGYPAWDTTAFARFMPMGELHQGSVRAFESNQGI
                     PTTKAHGIPITFDVPKGATSVQLWFRNASGAGMSCETWDSNLEANYVFAVQRFAPSPV
                     KWAGDWGNGFSRECEHRDGIEEPTVIDSWIMQRACMFVDADVYVPGLTDVDADPAGIV
                     AQVNYGFDDQDPVAGWLTYQGRVGNNYRYRWELPRDVLQQSSWDKITYRFRFSTDGLN
                     WYTIGQAQGPEGGDARTLLRSF"
     gene            complement(6812..7375)
                     /locus_tag="PK710_01115"
     CDS             complement(6812..7375)
                     /locus_tag="PK710_01115"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOT08330.1"
                     /translation="MTSSAKALPTQKGIDRRSSHLRAWLWAAVILAGFALLPRLASFP
                     SAMIGQPAPDFVLPVVLNGQVGEKVQLSQLSGKAVVLSFWASWCGPCRAEAPSVDRLS
                     RRLEDKGVKVLGINTSDDPDKAIAFARQKGLSFPIVSDEDGKVSQAFGVSSLPTIVVV
                     DKNGKVMAVRSGVTDESSLESLALSAR"
     gene            complement(7478..9094)
                     /locus_tag="PK710_01120"
     CDS             complement(7478..9094)
                     /locus_tag="PK710_01120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT08331.1"
                     /translation="MALLSRTTRKLTLAVLLAAVIPLAAAIIIARSLVSNVSAQLYNP
                     RVGEELERSLDLYKELAASLKSTMRYQAEAIAAQEGLRAAAMLNHRPSLEQELAEIFP
                     THTNLVQLAVVDGDGEIVVEKKRFTAVNVNTELALEVRKPLSNKADGPQLLAVFVTPK
                     ARFDERDQMEETVRTYRQIEASRSQVERAHLYAFTVLLVITMLAAVTLGTLLSRGVTR
                     RIGLLAHVAQVVGSGDLSVRVPIQGKDEITELAKAFNRMLREVQRSRARIEFLQRMGT
                     WQEMARRLAHEIKNPLTPIQLAVEEIHARYQGDDASFRKLLETTHGVVTEEVASLRRL
                     VTEFSAFARLPRADLSNHDICVFLREQQKHMVLFTDDESGILRTDGDVLERAVDVSWE
                     IATRPIAVYMDQQMMHRVLVNLVRNAAQAVSVKQSARGRVRVRLREVDKDWITIEVDD
                     DGPGIPIEMRETIFDPYVTTKADGTGLGLSIVKKIVMEHGGSIEATESEWHGARLVVT
                     LPRAGSSASFAARAQVGQSLQMTGMTMGER"
     gene            complement(9200..12349)
                     /locus_tag="PK710_01125"
     CDS             complement(9200..12349)
                     /locus_tag="PK710_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5808 family serine peptidase"
                     /protein_id="HOT08332.1"
                     /translation="MRGIERLTKLLILFSAFALSTYFALQFRSGGLWHGIEPAHAVAA
                     RSAVDAKTPYDLTKLEAVTATIQTIRDKYVDPTRVKPKQMLLSALDFVQRDVAQVIVL
                     RESNSDEVTVRVGSQQMVFDVSNVQGPWDVSAKLRDIFAFLQQHLKTDTDVDLREVEY
                     AACNGMLNTLDPHSVFLSPEAYREMTISTQGAFGGVGIVISIRDQLLTVIKPMPGTPA
                     GRAGLKQYDRIVRINNESTLNMPLDDAVRRLRGDPGTEVSVWVTREGPGGWSGSKPFK
                     LRREVINVESVESRMLDQGVGYIRLKQFQAGTAGELDNALVALHRAEPKMRGLVLDMR
                     SNPGGLLDQAVKVADRFLRSGVVVTTVSASEPRDEKVAKSPGTEPDYPLVVLVNSSSA
                     SASEIVAGALKNHNRAIIVGQKTFGKGSVQLVFPDVTREKAALKLTIAQYLTPGDISI
                     QSVGITPDIELDPMTVDTLEMDVMRPGKRMSERDLSKHLSSARAAAGQKPLEIVRYNM
                     PSAEREAMRERGGDPEDDFTMDFTTRFARDLVLNVPTGLSRVEAVRKAAEFIDKVRQD
                     EMAKVALDLKHLGFDWSATPAESPGPSKDQYEVKVETNRPNDRVRAGESIELKVTVKN
                     TGTEPVYQLRALTSAESSYYDRKELIFGKIEPGKSKTAIAPLGWCDVEGRRIGSTKKP
                     KDGKRVCTIPKDATTRCDGVKVEFFSANGEPPKSVEIRPTIEELPAPSFAYAYQIIDN
                     RQGANADGRLQRGEGVSMYLTVKNVGRGRSFETQANLRNLSGDGLALQAGRFDISNMQ
                     PGDMRSVVFTWEVQQVAEPEITIELSVGDRDLGKHVNEKIKIPVEAGLSLAQDVGVSK
                     VNGTEAPLVESLRPQARLFGSIPGGTLVRRLGRHGDMIKIDLGANRFAFVPASALSDA
                     PGAKAPDTISFDTVFSHSPPAIELTPAALVVRGPTVKITGSIVDQTRLLDAYMFVGSN
                     KVFYRTNQDAADPKSMKIDMDVSLRPGMNAITLVGRQNADTMSRQTIMIRRDGASGEL
                     LPSPKDDGAALLDFVEDGP"
     gene            complement(12787..13917)
                     /locus_tag="PK710_01130"
     CDS             complement(12787..13917)
                     /locus_tag="PK710_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOT08333.1"
                     /translation="MALSREIKVGVFVLLGLILSGGVVFLIGDQRNMFARKIDFVSTF
                     TDVQGLAAGAPIRMNGINVGSVSSVGHSEDLGDPRIHVKLWIVRSEALRLRVDARARV
                     VNKGLLGDKMLEIDPGKASQPLGLGGTIDSEDPTDFSNLVGQVGSIAERTNSVLGNLD
                     KASASFADDKVQQDIRGSIQSVNIILKHVAQGDGYANRLLTDPQEAERISRAVANLEK
                     TSNELSVAAAEVRQLVQRVNHGPGFAHSLVYGQEGADTVKSFGGAADEVTATLRGIRE
                     GNGLARAMLYGGTGQEAEIISNLNTASADLRDIMSGLKKGKGTLGALLVDPSVYEDMK
                     LVLGNVERNEVLRALVRYSIKQDEQRPAVEIKDPGAPASSAR"
     gene            complement(14005..14787)
                     /locus_tag="PK710_01135"
     CDS             complement(14005..14787)
                     /locus_tag="PK710_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT08334.1"
                     /translation="MNTEPLIQFRGVHKAFGNKVVYAGLDLDVYPGETLTIIGGSGVG
                     KSVMIKMLIGLLKADKGSIRFRGQEITTMSERELQTVRQRIAMLFQGAALFDSIDVGE
                     NVAYGLREHFRKKMSEQDIRERVAWALGLVGLSGIETMAPSDLSGGMKKRVGLARAVA
                     LRPDVVLYDEPTTGLDPINTTRINHMITGLRRVLGITSVVVTHDMASAYSISDRIAMV
                     HRGRIIATGTPEQIRASDNPHLADFIRGNAPENEDLDTLLTT"
     gene            complement(14815..15633)
                     /gene="phnD"
                     /locus_tag="PK710_01140"
     CDS             complement(14815..15633)
                     /gene="phnD"
                     /locus_tag="PK710_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOT08335.1"
                     /translation="MRFPILRFGVVPAVDDPTFRANLKDMVKALGYHLGTTVECRVTP
                     TYSALVALMTQGQLDAAWVPPVLAMDFDAQRIAHPRIAMIRQNETVYHSVLYALRDAQ
                     IRSVSDLQGAKAAWVSPDSASGYLVPLASMRARGLSLTKAFSDQVFLGTHEAVARAVA
                     HGQAHVGASFAHFEPSDFGTPVWASWIEFGLEVNFRVLLVAGPIPTDVISMHRSLDDQ
                     HQQAIIHAFSLLTSDKQTSEAARAIFRCEGFAGCTEEHLRGLRTLLRLLDRRRG"
     gene            complement(15725..16426)
                     /locus_tag="PK710_01145"
     CDS             complement(15725..16426)
                     /locus_tag="PK710_01145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HOT08336.1"
                     /translation="MNNTLQDGSGQMFDRICERYDFLNRVMSLGIDGRWRKRAVQALG
                     SDVGYVLDLATGTADVAIEVARRIPNSRVLGLDPSDGMLRVGQRKVEKAGLANRVTFK
                     KGDGQRIAASDDAFDAAIMAFGIRNVARRERCLRELGRVVRPGGPVVVLELSEPRGGH
                     PLAFGAGLWKRYVVPRLGSALSSTPEYAYLERSIRAFPRPDEFLELMATCGLRGGKAE
                     PLTFGAATLFTARAP"
     gene            complement(16500..17147)
                     /locus_tag="PK710_01150"
     CDS             complement(16500..17147)
                     /locus_tag="PK710_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOT08337.1"
                     /translation="MNRFNNKIGAITLVGLAALYMILSLSMGRSAASEAPPNLAPAEA
                     AVDVWQATALLAQQPQPVVVDVRAKDQYEQYHLTGSTSMPGASAHDILKVSQGKPAIL
                     LVADTDENASKLAGEVAALDKTTHVHFLKEGVRGWYLTYEVPTPLFNEKPPPYGWAEA
                     MATVQAFVHEGKGNASEVVAAVGKLSTSGYMPTQLQGKKKPAAAGAKKKVSGGCG"
     gene            complement(17144..17764)
                     /locus_tag="PK710_01155"
     CDS             complement(17144..17764)
                     /locus_tag="PK710_01155"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOT08338.1"
                     /translation="MQAPLASTLQWTHGMQMEMAVLVGIGFGFVLERAGFGRADNLAS
                     IFYGRDFRVMRVMFTAIVTAMIGVYFLDLIGFMPIGDIGLLPTYWLPQLIGGLMLGAG
                     FIIGGYCPGTSLVAVASGKIDAIFFVGGIFIGSSAFTLAYPSLEKFHESTSMGRVLMH
                     EWMGIPSGVMVLLVALFAVGSLTAVTKIEAMVRKSLESKQLPEVSS"
     gene            complement(17784..18299)
                     /locus_tag="PK710_01160"
     CDS             complement(17784..18299)
                     /locus_tag="PK710_01160"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOT08339.1"
                     /translation="MSKQREFWNPYAAGVALGLVLLASFVLTGQGLGASGAVTRVQAS
                     LWHALDPTWATENANIGKFFLSDTGALNTWIVFLVVGVALGGLLGALSAKRVKFETIH
                     GPRIGRDMRWVFALIGGIISGIAAQVARGCTSGQALTGGAQLALGSWIFMFAVFGGAY
                     GLAYFVRKQWI"
     gene            complement(18296..19756)
                     /locus_tag="PK710_01165"
     CDS             complement(18296..19756)
                     /locus_tag="PK710_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOT08340.1"
                     /translation="MRYGMMIELDKCVGCLACVSACKERWDSGPGVARDWVATFEHGS
                     REKNNLGVTFYPGLCMQCEDHPCTLDCPTGATYADENGVVIVDPEICIGCGNCVTMCP
                     YGARTPDPSKGIVEKCNLCAPYVANGGQPACVTTCLAECRHFGDLDDPNGSLMQLIQK
                     RGAKTLTTPQVDVGPKVYYAPESHRQQILAQGVIREPTPTWLTKVWQGVTRPVAQLGV
                     PAFAVIAGAGGLLMNLRHRGENKQPQSQVESEATGTLPRHRFGMRFLHWFNALSWVLL
                     LLTGTALMAAPSFALFGVGFPKAISTVFGGTSNLILFHVLWGLLWTAVIIPPFLFFKR
                     GGIEALREVMLTRDDIRWFMLKPLVMLGIGNHQLPPQDKYNAGQKVFAVSALVGTATI
                     IGTGLVMTFHLGSSHVVAAAILVHKLAVGLALVGVAVHITMAAIMKEERPALMSMIKG
                     DIDRRHAEHHSSKWVEELAHESAAESSVEPVGEKKS"
     gene            complement(19741..21990)
                     /locus_tag="PK710_01170"
     CDS             complement(19741..21990)
                     /locus_tag="PK710_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOT08341.1"
                     /translation="MSRPKEGAVSRRTAMGVVGAGAAVGVLTTARDAHAFRVLRSPEV
                     TPVERTIPTFCELCFWNCGVLADASNNRVLALRGHPDSPTAKGRLCGRGNAGAGFLRD
                     PDRLKYPMVRVGERGEGKFKRVGWKTAYQMVARAFDKIKKEHGAKALALFYHGSGGPM
                     LRNMLVAYGSPNYAAPSYAQCRGPRDVGYKLTIGRGPGSPEPLDFDKTQCIVLFGSHL
                     GENAHNSQVQEFVQARARGASLIVLDPRMSTVASKADVWLPLKTGSDTAILLSWMHLL
                     IANDTYHHTFVENQCTGFERLAEHVKPFTPSWAAQQADVPAEDIVKAYELMVKAMPAV
                     VVHPGRHVTWYGEADTQRARAQAILSALLGAIWAPGGLYRPAPPPALKDFPSPDFPEL
                     PANVDKAASRFPFATEVSTTGIRDATRTGVPYPIKGWFVHGSNLIQSLPNQHETIEAI
                     RSLDMLVVTDIQPTEITDWADVVFPEDNYLERYDDLKTGGNLRPYIAIRQPVVRSPYD
                     TRPAWRIAKELGTELGVGDFFAFDTFEEYLSARLDGSGVTLAELKEKGIYFPQSKVEP
                     YLDPQKPFAWQTPSGKVELFSKSLAEAGFDPMPVHKPPPDPPKGMLRLTYGRSPLHTF
                     GRTQNNDILHDLNPGNCVWLHPMCAASLGVQHGKPVFVQNAYGDVTGPMPARVTERIR
                     PQTVYMIHGFGHSAKGLSNACCMGGSDAELIRGYQVDGISGSTGMRTEFVKITPADGG
                     KEKYPCAMG"
     gene            complement(22108..23091)
                     /locus_tag="PK710_01175"
     CDS             complement(22108..23091)
                     /locus_tag="PK710_01175"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT08342.1"
                     /translation="MIVHRRFLLAGIISLILWISASSSQAAVIERVVAVVGDHPILMS
                     ELRQRARPFLLEIHSKIPTEAQRAAAESETLRQMLQRMIDDQLEAQAAAKMRIRVEAE
                     EIDKAIARLARMQDTTVQELLSEVLRSGMTAQEYRNEIRRQLLEGKLLELRVKGQVRI
                     TEEDMRALYHRLAREERRTLGYAPQWIVLRIPPDASAEVKAQRRALADEIAREARTGK
                     DFSQLARRYSDDSTSRQQGGHLGMRKPGELEVAIERVAIALEVGEVSAPFRFADALIV
                     MRVVEREQSRLGTYEQARNQLAQRVYAEKLETAKRRWLDNLKRSTHIDVRL"
     gene            complement(23148..24107)
                     /locus_tag="PK710_01180"
     CDS             complement(23148..24107)
                     /locus_tag="PK710_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOT08343.1"
                     /translation="MSNDLYKELGVTRTASANDIKQAYRKLASKLHPDKNPGDASAEA
                     RFKRVNQAYQVLSDKKRRALYDEFGDAALSDNFDAEQARAYRQWTQGRGGGGGRVSGV
                     QGVDLEDLFGANLGGQNIGDLFGNLFGGAGRKRASRTASMRGSDLESPLTIDFVSAVR
                     GSTVSLRIEGQAEPIQVRIPPGAKQGSKVRVKGKGSPSPFGGPPGDLLLELHVEPHPY
                     FHMQGDDLHVDLPITVTEAYRGARVTVPTPEGTVTMKVPAGSQSGQSARLRGKGMARR
                     GQPPGDLYVHFQVQVPTSQDKAVHQAMETLSQYEQDVRGELRF"
     gene            complement(24171..25115)
                     /locus_tag="PK710_01185"
     CDS             complement(24171..25115)
                     /locus_tag="PK710_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HOT08344.1"
                     /translation="MNVSKAIEGRRFLFLTGKGGVGKTTTTAALALVLASQGKRVLCA
                     TCQAKDRLSTLLEGPPVGEEIVQLRPNIMAVNIHPEAALREYGLMVLRLEVLYKAVFE
                     NRLVASFFRATPGLFEWAMLGKAWYHTTERRQDGQPRFDVVLFDAPSTGHALDMLRGP
                     KVIVDMAPPGLLHREAMKAWSMFTDPTQAGVVLVTLPEEMPVTETFELYEALDKELGL
                     PVAQLIVNGVIPPMFSASEREALLAPRDLSGTCLGDEALAAGVRRARRELVQHEALVR
                     LREGMGASDKSVPLAYLPHRFEGVNTFESVSELAMRLT"
     gene            complement(25141..>25608)
                     /locus_tag="PK710_01190"
     CDS             complement(25141..>25608)
                     /locus_tag="PK710_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08345.1"
                     /translation="AAKLPAPATLPTAATRLPAENRIALGIRFEPKDVFGVQGGGAGA
                     FGYYQHEGKRYRVVSITRDDADQAKDLLTALSKREGVTKLKDLGEGGVRMMEGEPGDV
                     RIEWVMARSGPQVFGIGDESSSLRAEMSATDHDAVCLTKEQKVERLKALLSVK"
BASE COUNT         5570 a         7879 c         6619 g         5540 t
ORIGIN      
        1 aattcatcat cagagcgccg ttgctcctcc tttttgttct cataaacctc caccgacaca
       61 tcgtaccgtt cagtctcaaa gtttgcggaa agcgcgatgg agtgatgacc agccacatcc
      121 gatccggtgg tttgcaccgt gagcgcctgt ccataagtgc ctggtccata actcaacgta
      181 atcgcccgag gccataacgt cgaaagcgga ttgtaggggc gtcgttccca atttgttcgc
      241 gaaggttcca ccggcgcttg gggccgctcg ggaacataag gcaacggttc caaaaaacgg
      301 ctcgcctcga aaggcatggc gaacaggtcg tagccaaacg acgtataacc aacgtatacg
      361 agcgttgttc cgtcaggaga aagctctggt tggtatgcac ccgttctgac gttggtcact
      421 tggtgcaatg cgcctgtcgc aagttcgagc gcataaatat tggcgatgcc cgtgcggtcc
      481 gacgtgaaca gcaaatactt gccatcgcgc gaataggacg gctgcatatc catcgcgcga
      541 tcgtgctgaa gttgttggaa ttgtcctgtg gccacttcga cgatgcggat gtcccggtag
      601 cctcctcgtg tccaaacgct gtaggcaacg tgtttcccat cgggagaaaa ccgcggtgta
      661 tacgcctgct cgaagcgcgc actcggcacg agtgtgcggg aatctcggat cgccccctcg
      721 gaggaaatat cagcgatctt gagataggac gtccctcgtg tattcacaac gtatacgatc
      781 tgccgaccgt cggggctcac gtccggatct cgcgcgcgat ggccgaaggt caaacgttgg
      841 atcgaaggtt ccgtgcccga cggcgcgcgc tggtgtgggc caatgcgatg aagatcgttg
      901 aaaaagtact ctcgacgcca cggttcgagg gccgcgtaga tcatcgttcc atccggtaaa
      961 aaagaggcgg aacactgacc atcggcgcga acgatcaacg gttcctccag cggttgcgcg
     1021 cgtcgtgacc cgtccatgag gacacgataa aaccccgatc gatcatggct gttgccccca
     1081 tgaaatatga cctccgtatg ctcggcttgc gttcgagcag ccggaggaac gaaacgcgga
     1141 ttcgatatcc actcccctcg cgtcgtcaga cgagttcctt cgcgaagccc ccgacgatac
     1201 accggatcca gttgcttgcg ataatgctcg cggagatact gctgccaagc cacgaatagc
     1261 tcttcgtagg ttcgtccggt gactcgacga atattgcggt tcaacccgta aggaatgagt
     1321 tgtgaaccgg tgtccgctgc cacggcagca aagacatcat agccataaat gtcctgaatc
     1381 cagcttagga agcgagagcc gtacaggtac cagacattgc cttgcggcca acggcggggt
     1441 gaatgcgaca tctggtcgag ccccatcaac cgttcgtcga gtacgtcagc gcgaaggaac
     1501 atatcgaaca tggtcgagcg gattcgcccg ccggtcgaat ggcgagactc gtggatgatg
     1561 gcgaagccct cgaggatcca tcgtggttgt gcttgattgg gcgcaaaggt gcggcctact
     1621 accgcgttga tgagcgctgg tacccccgtg atgttgtcca tctgcaagat atgcgtgtac
     1681 tcgtgggaca acaattccaa ataccaatca tcataatcgc caaggggcga catatcgtcg
     1741 ggggcggtta cgaatagacg caccgtgttg tagggaaatg ccgtggcgga accattggcg
     1801 ctgtccgtat catcggtgat cacgatctgc accaaatgcg atggtgacca acgaaggcct
     1861 tctcccatct cctcatgcag accttcgtac caattggcta cacgctcggc gattggctcc
     1921 aatcctttgg gataatgaat gcgtgcgtga ggcgtgtgaa gggtccacca ctcgagatag
     1981 ggatcaccgg cccaaagaac ggaagacgac aataggatcc acaacgctat cgaatgagcg
     2041 aacaggatac ggagcgcacg gcaccgcatg atcgcgatca aagcatcccc ccaagccgaa
     2101 gtggtcgggt gtggcactat aaatcgtcgt ggaataaagg ttcccgattg cgaacgtacc
     2161 ggtacacgag ctgacgagct tcggtggaga tatgggtgaa ttgaatgcca catcccggcg
     2221 aggtggcatc cgatgtcatc gattcacgcg attcgcgggt ccatcgaacg atgccgatgg
     2281 cttcgaactc atagccaccc ggcaacgaaa cctgcacttt gagggtttgg cccaccgcag
     2341 gcacattgta ggtagcaatg aaaaggccgc cgtgatcgac cacatcgttg ccggaaagcc
     2401 ctcgatagaa gttggtggcg gagtgcgttc caagttcagc ttcgatactc ggtaggtttg
     2461 ctgctcctcg atacggttca gcagtgggca ctgccggttt ggcagcgaac ggatcggctt
     2521 cgctccaact gctcgctgcg gcaggcatag gcgccgccgc aacctttggc gacgcattgg
     2581 ccggcccaac agccgccatc ggagccgatg gcgcttggaa ttgaggaact tggggtggcg
     2641 gctgaaaaaa agcgggctgc ggcggcggtt gatacggcgg cgcttgcggc accgggggtt
     2701 gatagggagc ttggggtggc ggttgaaaag cggggggctg tggcggcggt tgatacggcg
     2761 ccgcttggtg cattgggggt tgtgccacca cgggctgcgg tggcgcttga tgaaccggag
     2821 cttggggaga tggcgccgct tccgcctcgg cggatttcgt caacccaaaa atggttccaa
     2881 gcgacgatgc cacggcttcc atggtttgca tcatctgagg atccatggct gctttggact
     2941 gcagaatgga aagagcggaa cgcaccgcct cgagcgcttc cgtggcagac gttttgagta
     3001 cgggcccgcc catggtctca atgcgatgca gtgctcccat ggctttggca atgggctctg
     3061 ctgcttgcaa atactccggc ggcgcggtcg gattcgattg tagtagattc aaccccttgg
     3121 caagggattc cctggctgag cgtgcgttcg atacgatgtc ggacatttcc agctcccacg
     3181 gctacgttat catcattttg cgataaggga tgcaatctga tgagcaatgt cgcttgcttg
     3241 ccaccgatgc ccttcaaact cgccttgaag aagcccaact ccctcatcca aggcacgaac
     3301 cacagctact ccgccgatca ccgccgcttc tcctggtcgc aataccgcga agcatggacg
     3361 agtatcagct ttcaatagca ccagcggcat ttctcccacc gaaacgatag cccctggatt
     3421 gggggccaac gcccgaacac gtcgtacaat cgtgtgtgcc gtccatttcc acttgacgat
     3481 gcgatcttct tcggtaggga agggggccaa ggtggctttc gactcctctt gagccacaga
     3541 atcgatcggc tcgcctcgat caagagccgc tacggtctgt cgcaacaacg atagagaggg
     3601 gcgatcgagt tttttggcaa gatcccatgc gttccacgaa ggatcgatgg gtagacgccg
     3661 ttgcgccaag atcgcccccg tatcatagtc ctcctcgatt cgatggacgg ttacacccgt
     3721 cacatcgtct cccgcatcga tcgctgcaaa aaacgggtcc ggccctcgat gacgcggaag
     3781 tagcgatgga tgcaccccga ttcctccccc cttgcaggca tgcacaacct gcatcggaat
     3841 ccgacgcgtg aagtaccaac tcaccaacaa atccggttgc aaccgctcga gttgggcaag
     3901 atatccagcc caatgatggt gaggatcgag caccacccgc tcggcaccaa gcctggctcg
     3961 aagccgtcgc tctcctgtac gccaccgtcc gctcaaaccc gcaaggacaa gctcatgccc
     4021 atcctcgagc aaaagcaaag ccgcaaccgg aagaccgaga aatgcgatcc gcattcaacc
     4081 aaaccgataa tgcttgcgca cggcgcgtcg aacctcccat acgcactgca ttcccgcggg
     4141 aaacgtgacc aaatcacctt gtcccatggt gaccggtgtc cccccttgcg gcgtcaccac
     4201 gacctgcccc tcgagaaaat aacagatctc ttgctcctca tacgtccagg gaaacgtgga
     4261 tggctccttg gtccattgcg gccacgatgt cacctgcaac gcttgaagtc gggaatcgga
     4321 aggatttcgt tcaaccaata tttgcatgaa gttgcggtac ccagtgagtc gatgagcgtc
     4381 aagatgccac gccttatgct aggctctcca ctcgccgtac gttttttcac cagtcgattg
     4441 ccatggcaat ggggcccgag gattgaccaa agacgcaaac aagcaaactt cacctgctcc
     4501 tgagttgacg agcgcgctcg aggattatct agagacgatc tacgaactcg tgcgcgatca
     4561 aaaactggct cgcgtcaagg atatcgccaa agcccgagga gtccgggccg ggtccgtgtc
     4621 ccccgccatg cgacgtctgg ccgccatggg attgatcgac tacgcccatc gagaatacat
     4681 cgaccttacc cccgcgggag aacggttggc gcgacgcatc tatgcgcgtc acgaactgct
     4741 cacgcagttt ttcgagaaaa tactccagat gccaagcgat gaggcgagag ccaacgcgtg
     4801 ctcgatggaa cacagcctct caccacaagg catggaacac ctcgctcgat ttttcgagtt
     4861 tcttcaaatc tgccccgatg gtcccgaact gctcgcaaaa ttccacgcat gttccgtcgt
     4921 ccatgaaggc tcccccgact gtctcggctc gtgtcaaaaa gtacgaaatc agcaaaaaaa
     4981 ctcccaagaa cgattgttct ctttatggga cgtgcctcat gagcgccaag cccgcgtggc
     5041 gcaagtcgaa gcctcggcac aagtacgaca aaccctgctc gaccgaggat ttttgcctgg
     5101 aacacgagtg gtcgtcatcc ggcgatccgc cgatggtcag tcggtgtggg tgcaaatagg
     5161 cggattcgaa ctgcaattgg aaaaaaacta cgcgcaaaac gtgctgatga tcctcgaaga
     5221 ctgaaaccac agacgccgat cctgcttctg gcgcttcttt ggtagcgagt gggcttttgt
     5281 aggtgtcggt gcgattgtgc ttgcgtgtgg attcttctat gctattcgtt actcagatct
     5341 gagttaccat gaggtcatca tgaatcgaat catcgctttt ctgcctctcg tcgccattgc
     5401 cttgtcttcc tgttcggccc ctgtcgatga cgctgactca ccggcttccg tttccgccgc
     5461 gcaagttcca gcccccctcg ttcaaaaagc cgatagccaa gatgtcgcgg atcatggatg
     5521 cctcgtcgtg ctgcgccagg tgcaacgcag ccccggaccg accggctacg aaatacaatg
     5581 tacgtcagag cgttgttggt tcgtctggtc cggcacgatc gatgtggccc gttcgcttgt
     5641 cgaacaggga gcgacagtcg ggttgatgta tcaagtcggc atgggtgagt ggtgggaagt
     5701 ccccgccgag gcgacggagt cggatgatgg cccaatgcag tccttcgcct ttcgtttctt
     5761 cgatcatgcg gtggatacct cgctcagctt gacggctttg gcgcaagctc gcgttcaagt
     5821 cattccggtg gtgcatttgc ccggcggggg gcgcttgttc gatcacaatc gtgtgtccag
     5881 cgatttggac aattacgttt tgaactatgg caacgattgg tcggtcaacg atgacgattc
     5941 ggtgtgtacg gtcggcgccc ccacagcctc gacgctggag tttttggctg gatggaaaca
     6001 cgtccagcat ggaacgatcg tcgaaggggg aaccttgacg attcaatacg atcttgcgcg
     6061 tttgcccgat tgccgcggaa cgcataacgg ctatccggct tgggatacaa cggcttttgc
     6121 tcgattcatg cccatgggcg agcttcacca gggaagtgtt cgtgccttcg aatcgaatca
     6181 aggaatccct accaccaagg ctcatggcat cccgattacc ttcgatgttc cgaaaggagc
     6241 gacgagcgtg cagttgtggt tccgcaacgc gagcggagcc ggaatgtcat gtgaaacctg
     6301 ggacagcaac ctcgaagcca attacgtttt tgccgtgcaa cgtttcgcgc cttcgccggt
     6361 caagtgggca ggcgattggg gcaatggctt ctcgagagaa tgcgagcatc gagatggcat
     6421 cgaagagccc accgtgatcg attcctggat catgcagcgt gcttgcatgt tcgtagatgc
     6481 cgatgtgtat gtccccggat tgacggatgt agatgcggat cccgcgggga ttgttgccca
     6541 ggtgaactac ggcttcgatg atcaggatcc ggttgccgga tggcttacgt accaaggccg
     6601 tgttggaaat aattaccgtt atcggtggga attgcctcgc gacgttcttc agcagtcgag
     6661 ctgggacaag attacgtatc gctttcgctt ttcgaccgat ggtctgaatt ggtacaccat
     6721 cggtcaagcg caaggccctg aggggggaga cgcgcgaacc ctgttgcgaa gcttctgatg
     6781 agcttttccg tttcgtttgc ggttcatcat ctcagcgtgc agaaagtgct agtgattcga
     6841 gcgagctttc gtccgtaacc cctgaacgca ccgccatcac tttcccattt ttatccacca
     6901 cgacaatcgt cggcagactc gaaacgccga atgcctggct aaccttgccg tcctcgtccg
     6961 atacgatagg aaaggaaagc cccttttgcc ttgcaaaagc aatggctttg tcagggtcat
     7021 cgctcgtgtt gatcccaagc accttcacac ctttgtcttc gagccttctg gacaatcggt
     7081 ccaccgaagg agcttccgcg cgacaaggac cacaccatga tgcccaaaac gacaagacca
     7141 ctgccttccc agacaattgg gatagctgca ccttttcgcc tacttgtccg ttgagcacga
     7201 cgggaagcac gaagtccggc gcgggttgac cgatcatcgc ggaaggaaac gaagccaatc
     7261 gcggcaacag ggcaaaaccg gcaaggatca ccgcagccca tagccatgct cggagatgcg
     7321 acgaacgccg atcgattccc ttttgcgtgg ggagtgcctt tgctgaagat gtcatgagat
     7381 atccagacgg cccgggccga ccttggttgg agatgggtcg cgcctaaacg attcctggcg
     7441 ttgaataacg gtatcgtcaa caacggatag cgcgccctta ccgttctccc atcgtcattc
     7501 cagtcatctg cagggattgc ccaacctgtg cgcgcgccgc gaaagaagcg ctgcttcccg
     7561 cgcgaggcaa cgtaaccacc aaccgtgcgc cgtgccattc actttcggtg gcctcgatgg
     7621 acccaccatg ttccatcacg atctttttca cgatcgaaag gcctaacccc gtaccatcgg
     7681 ctttggtggt gacatacgga tcaaagatgg tttcccgcat ttcaatcggg atccccggac
     7741 cgtcatcgtc cacctcgatc gttatccaat ccttgtccac ctcccgaagt cgaacgcgca
     7801 cccttcccct tgcggactgt ttgacgctga cggcctgcgc cgcgtttcgg acgagattga
     7861 cgagaacgcg gtgcatcatc tgttggtcca tatagaccgc gatcggcctc gtggcgatct
     7921 cccaggacac atccacggcc ctttccagca cgtccccatc cgttcgtaaa atcccgctct
     7981 catcgtcggt gaacaacacc atgtgctttt gttgttcgcg caaaaaaacg caaatatcgt
     8041 gatttgacaa gtcggcgcgg ggaagacgtg cgaatgcact aaactcggtg acgagacgac
     8101 gcagactcgc tacctcttcg gtcactaccc catgggtcgt ttcgagaagc tttcgaaacg
     8161 atgcatcatc accttgatag cgtgcgtgaa tctcctcgac agcgagttgg atcggggtca
     8221 gcggattttt gatctcatgc gccaatcgcc gcgccatctc ctgccatgtt cccatccgct
     8281 gaagaaactc gatacgcgcc ctggagcgtt gcacctcccg gagcattcga ttgaaagctt
     8341 ttgccaactc cgtgatctca tctttgccct gaatgggcac tcgcacggaa agatctcccg
     8401 aacccaccac ttgcgccacg tgcgccaaca gcccgattcg tcgcgtgacg ccgcgagaaa
     8461 gaagtgttcc caaggttacg gcggctagca tcgtgatgac cagcaagacc gtaaaagcgt
     8521 agagatgcgc gcgttccact tgggagcgcg aagcctcgat ttgacgatag gtgcgaacgg
     8581 tttcctccat ttgatctcgt tcgtcgaatc gcgctttggg cgtgacgaat acggcgagca
     8641 attgagggcc atctgctttg ttcgaaagag gttttctcac ttcgagcgcg agttcggtgt
     8701 tgacgttgac ggccgtgaaa cgtttttttt cgaccacgat ttcgccatct ccatcgacga
     8761 cggccagttg gacgagattg gtatgagttg gaaaaatctc ggcaagttct tgctcgaggc
     8821 ttgggcgatg attgagcatc gccgcggcac gcaggccctc ctgtgctgca attgcctccg
     8881 cttggtaacg catggtcgat ttcaaactcg cggccaattc cttgtataga tcgagagaac
     8941 gctccaactc ctcgcccacg cgcggattgt atagctgcgc ggaaacattg ctgaccaagc
     9001 tacgcgcgat gatgatcgcc gccgccaacg ggatgacagc agcaagcaac accgcaagcg
     9061 tcagctttcg cgtggttcga gataacaacg ccatcgaaaa agtagtgtgg cagatgtcga
     9121 catgggcact caacccgtgt cgtcatccac tcgccagatc atgtcgaaac agcggccagt
     9181 ccggtcgcct gcccagccat caaggaccgt cttccacgaa gtccaacaac gccgctccat
     9241 catctttcgg cgatggcaat agctctcccg acgctccatc tcgtcgaatc atgatcgttt
     9301 gacgagacat ggtgtcggca ttttgccttc ccacgagcgt gatggcattc atcccgggcc
     9361 gaagagacac gtccatgtcg atcttcatgc tcttgggatc cgcggcatcc tggttggttc
     9421 gataaaacac cttgttggaa cccacgaaca tataggcgtc caacaggcgc gtttgatcaa
     9481 cgatgctccc cgtgattttt acggtaggtc ctcgtacgac caaagccgcg ggagtcaatt
     9541 caatggcggg aggtgaatgt gagaacaccg tatcgaaact gatcgtgtcg ggggctttgg
     9601 cacccggagc atcggataat gcgctggcgg gaacgaaagc aaaacgattg gctccaagat
     9661 cgattttgat catatcccca tgtcgtccaa gccttcgaac gagtgttcct cccggaatgg
     9721 aaccaaataa gcgtgcttga ggtcgaagcg actccacgag cggagcttcc gtaccgttga
     9781 ccttggaaac gccaacgtct tgtgcgagtg acaatcctgc ttccaccggg atcttgatct
     9841 tctcgttgac gtgtttgccg aggtctcggt ctcccacgct caattcaatg gtaatctccg
     9901 gctccgcaac ctgttgcact tcccaggtga acaccaccga acgcatgtct cccggttgca
     9961 tattcgagat atcgaagcgg cccgcttgga gcgccaagcc gtcgccggac aagttccgaa
    10021 ggttcgcttg ggtctcgaag gaacggcctc gacccacgtt ttttaccgtc aggtacatcg
    10081 ataccccttc cccacgctgc aaacgcccat cggcattcgc tccctgtcga ttgtcgatga
    10141 tctgataggc atacgcaaaa gatggcgctg gtaactcctc gattgtcggt cttatttcga
    10201 ctgacttggg aggctcaccg ttggcggaga aaaactcaac cttgaccccg tcgcaacgcg
    10261 tcgtcgcatc tttcggtatc gtgcataccc gtttgccatc cttcggtttt ttggtggaac
    10321 cgatgcgacg tccttccaca tcacaccaac ccagcggcgc gatggctgtt ttggattttc
    10381 ctggttcgat ttttccaaag atgagttctt ttcgatcgta ataagaactc tcggccgagg
    10441 tcagtgcgcg aagctgatac acgggttctg tccccgtgtt ttttacggtt actttcaact
    10501 cgatggattc ccctgctctc acgcgatcgt tggggcggtt ggtttccacc ttgacttcgt
    10561 attggtcttt cgatggtcct ggggattccg ctggggtggc gctccaatcg aatccaagat
    10621 gtttcaagtc caaagctacc ttcgccatct cgtcttgacg aaccttgtcg atgaactctg
    10681 ctgcctttcg caccgcctcc acacgcgaca ggcccgtggg aacattcaaa accagatcac
    10741 gcgcaaagcg cgtggtgaaa tccatggtga agtcgtcttc gggatctcct ccccgttccc
    10801 gcatcgcttc tcgttcggcg gaaggcatgt tgtatcgcac gatttcaagc ggtttttgcc
    10861 ctgccgcggc acgcgcgctc gacagatgtt tggatagatc ccgctcgctc attcgcttgc
    10921 ccgggcgcat cacatccatc tccaacgtgt ccaccgtcat cggatcaagc tcgatgtccg
    10981 gtgtgatccc caccgactga atggagatgt ctcccggtgt cagatattgg gcgatcgtca
    11041 gcttcaacgc agccttttcc ctggtaacgt cgggaaatac gagttgcaca ctcccttttc
    11101 cgaaagtttt ctgacctaca atgatggcgc gattgtggtt tttcagcgca cccgccacaa
    11161 tctcgctggc actcgcactc gaggagttca ccaataccac caacggataa tccggctccg
    11221 tccccggcga cttggccacc ttctcatcac gcggctcaga agccgatact gtcgttacca
    11281 ccactccgct gcgcaaaaac cgatccgcta ccttgacggc ttgatcgagc aagccccccg
    11341 ggttggaacg catgtcgagg accaatccac gcattttcgg ttccgcgcga tgcaaggcca
    11401 cgagtgcgtt atccaactcc cccgccgtcc ccgcttggaa ttgcttcaac cggatatatc
    11461 ccaccccctg gtcgagcatc cgactctcca ccgattcgac attgatgacc tctcgtcgaa
    11521 gcttgaatgg cttactgccc gaccaaccac caggtccctc ccgagtcacc cacacggaga
    11581 cttccgtccc agggtcaccc cgcaaacgac gtaccgcatc gtcgagcggc atgttgagcg
    11641 tagactcgtt gttgattcga acaattcgat catattgctt caacccggct cgccccgcag
    11701 gcgtcccggg catcggcttg atcaccgtta aaagctggtc tcgaatcgag atcacgatcc
    11761 ccacgccccc aaatgctccc tgcgtggaaa tcgtcatctc ccgataggct tccggactca
    11821 aaaacaccga atgcggatcg agcgtgttga gcattccatt gcacgccgca tactcgactt
    11881 cccgtaaatc cacgtccgta tccgtcttga gatgctgctg caaaaaagca aaaatatccc
    11941 ggagctttgc cgatacatcc caaggcccct gcacgttgct gacatcgaaa accatctgtt
    12001 gcgacccgac acgcaccgtc acttcgtcgg aattcgattc ccgcaataca atcacttgcg
    12061 ccacatcccg ctgcacgaaa tcaagcgctg aaagaagcat ttgcttgggt ttcacacggg
    12121 tcggatccac atacttgtct cgtatcgtct ggatcgtcgc ggtcaccgcc tccagtttcg
    12181 taaggtcgta gggagttttt gcatccacgg cgcttcgcgc tgccaccgca tgggctggct
    12241 cgatgccatg ccaaagacct ccactgcgaa attgaagcgc aaaatacgta gatagggcga
    12301 aagcactgaa taggatcaga agcttggtga gacgttcgat gccgcgcatg atgttcaaca
    12361 atagatgacg gatgcaaaag gaccaaaggc aaagcccagg gaattgactt gccgcgaacc
    12421 agggccggac gcaagtcacg acggcaagag cgtttatggc gaccttccgg aaacgaccat
    12481 cgatttcgtt gccccttgcg ggtagggcgc gtcaagagtg ccaacccatt cgtgggggag
    12541 agtcatcacc caggccgata cctcctcttt ggtgcgccgc gtggtctgta acatattgaa
    12601 ttatgtgtat atttctacaa tgtggcgcgt tggtgtcgtc ggaggcacaa ccccccccgc
    12661 cttcgaccct tggtatgggt ctttcggccc caacgtttcg tttgagtcta cccccatggt
    12721 tcgaagacat gggtacccaa cccgcacgcc ctgcgtgaac gttcgtcgga tggcgagcgc
    12781 atcggttcag cgcgcactag acgcgggcgc gccagggtcc ttgatttcca ccgcaggacg
    12841 ctgctcgtct tgtttgatcg agtaacgaac gagcgctcgg agcacctcgt tgcgttcgac
    12901 attgccaagc acaagcttca tgtcctcata cacggatgga tccaccagca aagcccccaa
    12961 cgttcccttc cccttcttca accccgacat gatgtcacgt aggtctgcgc tcgcggtatt
    13021 gaggttggaa atgatctcgg cttcttgtcc cgtacccccg tagagcatgg cacgagccaa
    13081 tccattaccc tcccgtatgc cgcgaagtgt ggcggttacc tcgtcggccg cccccccaaa
    13141 actcttcacg gtatccgcgc cctcttggcc gtagaccaaa ctatgcgcga atccgggacc
    13201 atgattcacg cgctgaacca attgcctcac ttccgcagcg gctaccgata attcattgct
    13261 cgttttctcc aagttggcga ccgcacgcga tatccgttcc gcttcctgtg gatccgtcaa
    13321 cagccggttc gcatagccgt cgccctgcgc cacatgcttc aaaatgatgt tcaccgactg
    13381 aatcgaacct cttatgtctt gctgaacttt gtcgtcggca aaagatgccg acgctttatc
    13441 caagtttccc aacaccgaat tggtccgttc ggcgatcgac cctacctgtc cgaccagatt
    13501 cgaaaagtcc gttggatcct cgctatcgat ggttccgcca agaccaaggg gttggcttgc
    13561 tttgccggga tcgatctcca acatcttgtc tcccaacaaa cctttgttga ctacccgcgc
    13621 acgcgcatcc acacgcaacc gcagcgcttc cgatcgaaca atccataatt tgacgtgaat
    13681 gcgcgggtct ccaaggtctt cggagtgacc tacgctcgac accgaaccca cgttgattcc
    13741 attcatccga atcggggctc cggccgcgag cccctgcaca tcggtgaacg tcgacacgaa
    13801 gtcgattttg cgcgcgaaca tattgcgctg atccccaatc agaaacacta ccccccccga
    13861 gaggatcaac cccagcaaaa cgaatactcc cactttgatc tctcgggata aagccattga
    13921 ttactcccag gggtacacgg cttagcacga tgccgctcgc agcgcgaaac atgcgcgggc
    13981 gagccccaaa gatgacgcga aaggtcaagt cgtcaagagc gtatccaagt cctcgttttc
    14041 aggagcattt ccacgaatga aatccgccag gtggggattg tccgaggcac gaatttgctc
    14101 cggtgtcccc gtggcaatga tgcgtccacg atggaccatc gcgattcgat cacttatcga
    14161 atacgcactg gccatgtcat gggtgaccac cacgctcgtg atgccaagca cacgacgaag
    14221 ccccgtgatc atatgattga tccgagtcgt gttgatgggg tcgaggccag tcgttggttc
    14281 atcgtacagc accacatcgg gccgaagggc cacggctcgc gccaggccaa ctcgtttttt
    14341 catccctccc gacaggtccg agggtgccat cgtttcaatt cctgaaagac ccaccaatcc
    14401 caacgcccac gccacccttt cccgtatgtc ttgctccgac atcttctttc gaaagtgttc
    14461 tcgcaatcca taagccacgt tttctcccac gtcgatcgag tcgaacaacg ccgccccttg
    14521 aaacaacatc gcaatgcgct gtcgcaccgt ctgcaactcc cgctccgaca tcgtcgtgat
    14581 ctcctgaccg cgaaaccgga tcgaaccctt atccgctttc aaaaggccaa tcagcatctt
    14641 gatcatcacg ctcttgccca ccccagaacc cccgatgatc gtcagcgtct cgcccggata
    14701 cacatccaaa tccaacccgg catacacgac cttgttgcca aatgccttgt gaacgcctcg
    14761 gaattggatg agcggctcgg tgttcattga tcccatcaat cccgctggat tccctcaacc
    14821 tctgcgacgg tccaaaagcc gaagaagcgt gcgcaatccc cgaagatgtt cctcggtaca
    14881 acccgcaaac ccctcacatc ggaagatcgc tcgggccgct tccgaagttt gcttgtccga
    14941 agtcaatagg gaaaacgcat ggatgatggc ttgctgatgc tgatcgtcca gggagcggtg
    15001 catcgagatg acgtcggtag ggatcggtcc agccaccagc aatacgcgaa agttcacctc
    15061 caatccaaat tcgatccaac tcgcccacac gggagtcccg aagtccgatg gctcgaagtg
    15121 agcgaaactt gcgccgacat gagcttgtcc gtgcgctacc gcacgcgcta ccgcctcatg
    15181 cgtccccaaa aaaacctgat ccgaaaacgc tttcgtcaac gacaggcccc gcgctcgcat
    15241 cgatgccaga ggcaccaaat atcccgatgc actatcagga ctcacccagg ccgctttggc
    15301 tccctgcaag tccgatacac tacgtatctg cgcgtctcgt aacgcataca acaccgaatg
    15361 gtataccgtc tcgttctgac gaatcatcgc aatgcgcgga tgggcgattc gctgtgcgtc
    15421 gaagtccatc gccagcaccg ggggaaccca tgccgcatcc agttgcccct gtgtcatcaa
    15481 ggcgaccaat gcggaatagg tgggtgtcac acggcattcc accgtcgttc ccaagtgata
    15541 ccccaaagct tttaccatat ccttgaggtt cgctcgaaac gtcggatcat ccacagcagg
    15601 cactacgcca aatcgcaata tcgggaaacg cacccgtggc accttgctcg tcagcgtcgc
    15661 tcaggtcaag ccatgcaacc gttcaaaccc gccagaaacc atttggacgc gatgcccatg
    15721 cagatcatgg ggctcgcgcc gtaaacagcg tcgccgcacc aaaggtcagc ggttctgctt
    15781 ttccccctcg taatccacac gttgccatca attcgagaaa ttcgtcggga cgtggaaatg
    15841 ctcgaatcga acgttccaga tacgcatact cgggtgtcga cgatagggca gaacccagcc
    15901 gagggacgac gtaccgtttc cacaacccag ccccaaacgc gagaggatgc cctccccgcg
    15961 gttccgaaag ctcgagcacg accaccgggc ccccagggcg aaccaccctg ccaagttcac
    16021 gtaaacatct ctcgcgccgc gcaacgttgc gaatcccaaa agccatgatc gcggcgtcaa
    16081 aggcatcatc gctcgcagcg attcgctgcc catcaccctt cttgaacgta acgcgattcg
    16141 ccaatcccgc tttttctacc tttcgctgtc ccacacgcaa cattccgtcg gacggatcca
    16201 gccccagcac acggctgttc ggaatcctac gtgctacttc aatcgcaaca tcagccgtgc
    16261 ccgtcgcgag atccaacacg tatccaacat cagatccaag ggcttgcacc gcacgttttc
    16321 gccaccggcc gtcgattccc aacgacatca cgcggttcaa aaagtcatag cgctcgcaga
    16381 tccgatcgaa catctgaccg gacccatcct gcaatgtgtt gttcatgtac gggaggctat
    16441 tagtcgattt gtaggcaagg acctagcttt tgtcccacgg cgtaggctcg tgcgatcgct
    16501 catccacatc ctccgctgac tttcttcttt gcccctgctg cagcaggctt ctttttgcct
    16561 tgaagctgag tcggcatata tcccgatgtg gatagttttc ccacggcagc cacgacctcg
    16621 gaggcattgc cttttccctc gtgaacgaaa gcttgcaccg tggccatcgc ttctgcccat
    16681 ccatacggag gaggtttctc gttgaacaaa ggcgtgggca cctcgtaggt gagataccat
    16741 cctcgcactc cctctttcaa gaaatgcaca tgcgtcgttt tgtccaacgc cgcgacctct
    16801 ccggccagct tcgatgcgtt ttcatcggta tccgccacga ggagtatcgc tggttttccc
    16861 tgggagacct tgagaatgtc gtgcgcggat gcgcctggca tcgaagtaga acccgtcagg
    16921 tgatactgct cgtactgatc cttggcacgc acatccacca cgactggttg cggttgctga
    16981 gcaagcagcg cggtagcttg ccaaacgtcc acggccgctt cggcgggcgc aagattcggt
    17041 ggcgcctccg acgccgcgga gcgtcccatc gataacgaaa ggatcatgta tagcgcggca
    17101 agccccacaa gggtgatggc cccaatcttg ttgttgaatc ggttcatgaa gacacctccg
    17161 gaagctgctt gctttcgagt gacttgcgaa ccatcgcttc gatttttgtg accgcggtca
    17221 atgaccccac cgcaaatagc gcgaccaaca gcaccatgac ccccgaggga attcccatcc
    17281 actcgtgcat cagaacgcga cccatggacg tactttcgtg gaacttctcg agcgaaggat
    17341 aggcaagcgt aaacgcggaa gagccaatga aaataccgcc gacgaaaaat atcgcatcga
    17401 tttttccact ggccacggcg accagcgacg tgccgggaca ataacctccg atgatgaaac
    17461 ccgccccgag catcaaaccg ccgatcagtt gcggcagcca atacgtaggt aatagtccga
    17521 tgtccccaat gggcatgaaa ccgatgagat cgaggaagta cacgccgatc atggcagtga
    17581 cgatggccgt gaacatcacg cgcattacgc gaaaatctcg accatagaaa atggaagcga
    17641 gattatccgc gcgaccaaac ccggcgcgct cgagcacgaa gccaaaacca atcccaacca
    17701 acacggccat ttccatctgc atgccgtgtg tccactgtaa cgttgaagcg agaggtgctt
    17761 gcatgatcct atgcctccct ttgtcagatc cactgcttgc gcacgaagta agcgagaccg
    17821 taggcgccgc caaacaccgc gaacatgaat atccaactgc ccaacgcgag ttgggctccc
    17881 cccgtcaatg cttgcccact ggtgcatcca cgtgccactt gtgccgcaat tccgctgatg
    17941 atgccgccaa tcaacgcgaa cacccagcgc atgtcgcgcc ctatgcgcgg gccatggatc
    18001 gtctcgaact tgacccgttt tgcggaaagc gccccaagca aacctccaag ggcgactccc
    18061 actacgagaa acacgatcca cgtattcagg gcacccgtat ccgacaagaa aaacttaccg
    18121 atattggcgt tctccgtcgc ccaagtcgga tcgagcgcat gccaaagcga agcttgtacg
    18181 cgcgtgaccg ctccgctcgc cccaagcccc tgtcccgtca gcacgaagct agccaacaat
    18241 accaagccca gagccacgcc agccgcatag ggattccaaa actctctttg cttgctcatg
    18301 attttttctc ccccacgggc tcgacagatg attccgccgc gctctcgtga gccaactcct
    18361 cgacccactt gctgctatga tgctccgcat gccgacgatc aatatctcct ttgatcatcg
    18421 acatcagcgc aggacgctct tctttcatga tggctgccat cgtgatgtgt accgctacac
    18481 ccacgagagc cagtcctacc gccaacttat ggacaagaat cgcggcagca acgacatgcg
    18541 atgatccaag atggaaggtc atcaccagac cggtcccgat gatcgttgcc gtgcccacca
    18601 gcgcgcttac cgcaaacact ttctgccccg cgttgtactt gtcttggggc ggaagctgat
    18661 ggttgccgat tcccaacatc acgagcggtt tcagcatgaa ccaccgaatg tcgtcacgtg
    18721 tcagcatcac ctcacgcaat gcctcgatcc caccacgctt gaagaacaaa aacggcggaa
    18781 tgatcacagc ggtccacaga agcccccaca agacgtggaa caagatcaga ttcgaagtac
    18841 ctccaaatac cgtcgagatc gctttgggaa atcccacgcc aaacaaggca aacgacggcg
    18901 cggccatcaa cgccgtgccc gtcagcaaga gcaacaccca cgatagcgca ttgaaccaat
    18961 gcaaaaagcg cattccgaaa cgatgccgtg gcaaggtgcc tgtcgcttcg gattcgacct
    19021 ggctttgtgg ctgcttgttc tcaccccgat ggcgaagatt catcaacaga ccgcccgctc
    19081 ccgcgatgac tgcaaacgcg ggcacgccca attgcgcgac cggtcgcgtg accccttgcc
    19141 ataccttcgt caaccatgtc ggcgtcggtt ctcggatgac accctgagcg agaatctgct
    19201 gacgatgcga ctccggcgcg taatacacct tcggtcccac atccacttgc ggtgtcgtca
    19261 gtgtctttgc cccgcgcttc tgaatgagct gcatcaacga accgttggga tcatccagat
    19321 cgccaaagtg ccgacactcc gccaaacaag tcgtcacaca ggcaggctgc ccaccgttgg
    19381 caacataagg cgcacataag ttgcacttct ctacgatgcc tttcgatgga tcgggtgtgc
    19441 gagcaccata cggacacatc gtcacgcagt ttccacaacc aatgcatatc tcggggtcca
    19501 cgatgaccac cccgttttca tccgcatacg tagctcccgt cggacagtcc aacgtgcaag
    19561 gatggtcctc acattgcatg caaagaccgg gataaaacgt cactcccaga ttgttttttt
    19621 ctcgggaccc atgctcgaac gttgctaccc aatcccgcgc aacacctgga cccgaatccc
    19681 agcgctcctt gcacgccgac acgcacgcca gacatccgac gcacttgtcc agctcgatca
    19741 tcatcccata gcgcatgggt acttctcctt accgccgtcc gcaggcgtta ttttcacgaa
    19801 ctccgtgcgc attcccgtcg atccgctgat gccgtcgacc tgatacccac ggatgagttc
    19861 cgcgtcgctt ccccccatac aacacgcatt ggacaatcct ttggccgaat gaccgaaccc
    19921 atgaatcatg tacacggttt gtggacgaat ccgctcggtg acccgggcgg gcatcggtcc
    19981 ggtgacatca ccgtacgcgt tttgaacgaa tacgggtttg ccatgctgca ccccgaggga
    20041 ggccgcgcac atcgggtgta accacacgca attgcctggg ttcaagtcgt gcaaaatatc
    20101 gttgttctgt gttcgaccga acgtgtgcag cggactgcga ccatacgtca agcggagcat
    20161 tcccttggga ggatcgggtg gcggcttgtg tacgggcatg ggatcgaacc ccgcctccgc
    20221 caatgatttg ctgaacaact ccaccttgcc ggacggcgtc tgccaagcaa atggcttctg
    20281 aggatccagg tacggttcca ctttgctttg cggaaaatag ataccttttt ccttgagttc
    20341 agccagcgtc accccagagc cgtcgagccg cgccgacaag tactcctcga acgtatcgaa
    20401 cgcgaagaaa tctcccacgc caagctccgt tcctagttcc ttcgcaatcc tccacgccgg
    20461 tcgcgtatcg taagggcttc tcaccactgg ctgacgaatc gcaatatatg gacgaagatt
    20521 gcccccggtc ttcaagtcgt cgtatcgctc gagataattg tcctcgggaa aaacaacatc
    20581 cgcccaatcc gtgatttccg tgggctgaat atccgtcacc accagcatgt cgagactgcg
    20641 aatggcctcg atcgtttcat gctgattggg tagtgattga atgagattcg agccgtgaac
    20701 aaaccagcct ttgatggggt acggaacgcc cgttcgtgta gcatcccgga tccctgtcgt
    20761 agaaacctcg gtggcaaacg gaaagcgcga cgccgccttg tccacgttgg caggcagttc
    20821 gggaaagtcc ggtgaaggaa agtccttgag cgctggtggt ggcgcgggtc gataaagccc
    20881 ccccggtgcc caaatggctc ccaacagcgc actgagtatc gcttgcgctc gcgctcgttg
    20941 tgtgtctgct tcgccatacc acgtaacatg ccgacccgga tgcaccacta ccgctggcat
    21001 cgctttcacc atcaattcgt aggcttttac gatatcctcg gcggggacat ccgcctgctg
    21061 cgccgcccac gatggcgtga acggcttgac atgctcggcc aatcgctcga atcccgtaca
    21121 ctgattttcg acaaacgtgt gatggtaggt gtcgttggcg atcaacaaat gcatccacga
    21181 caacagaatc gccgtgtcgg aacccgtttt gaggggaagc caaacatccg ctttcgatgc
    21241 caccgtcgac atgcgagggt cgagcacgat taggcttgcc cctcgcgccc gcgcctgcac
    21301 gaactcctgc acctgactgt tatgcgcgtt ttcccccaaa tgcgaaccaa acagcacgat
    21361 gcattgagtc ttgtcgaagt ccaacggttc gggactccca ggtcctcgac cgatcgtcaa
    21421 tttataacct acgtctctcg gccccctgca ttgcgcatac gaaggtgccg catagtttgg
    21481 gcttccataa gcaaccaaca tattgcgcaa catcggcccg cccgaaccat gataaaaaag
    21541 cgccaatgcc tttgcgccat gctccttttt gatcttgtcg aaagctcgag ccaccatctg
    21601 gtaggccgtc ttccaaccta cccgcttgaa ctttccttcc ccacgctccc ccacccgtac
    21661 catcggatac ttcaatctat ctggatcccg caaaaaaccc gcgcccgcgt tgccacggcc
    21721 gcataaccgc cccttcgctg tcggtgaatc aggatgcccc cgcaacgcca agacgcggtt
    21781 attgctcgcg tccgcaagca ccccgcaatt ccaaaaacat aactcgcaaa acgtcggtat
    21841 cgtccgctcc accggagtca cctccgggga acgaagaaca cgaaacgcat gcgcatcacg
    21901 agctgtcgtg agcactccta ccgccgcgcc ggctccgacc acacccatcg cggtgcgtcg
    21961 tgacacagcg ccttccttgg gtcgcgacat ggaagacact ccattcgagg gggcgggact
    22021 cgagcccaca acccacaacc gtgagagaca caaccttaag aaaagatttc gagaaattac
    22081 tatttgttgt tcacgtccac gctggaccta caaccgaacg tcgatgtgtg tgcttcgctt
    22141 cagattgtcg agccagcgac gtttggcggt ttctagcttt tccgcataaa cacgctgcgc
    22201 cagttgattg cgtgcctgtt cgtacgtccc caaacgactt tgctcacgct caaccactcg
    22261 catcacaatc aatgcgtccg caaatcgaaa cggtgccgat acctctccaa cctcgagagc
    22321 aattgccaca cgctcaattg ctacctcgag ttcccctggc ttcctcatcc caagatgacc
    22381 cccttgctgt cgagaagtcg aatcatccga gtaacgacgt gccaactgcg aaaaatcctt
    22441 acctgttcgc gcttcccgcg cgatctcatc ggcaagcgca cgacgctgcg ctttgacttc
    22501 cgcgcttgcg tccgggggaa tgcgaagcac gatccattgc ggagcatatc ctaaggtgcg
    22561 tcgttcttcc ctcgcgaggc gatgatacaa cgcgcgcatg tcttcttcgg tgatacgcac
    22621 ctgacctttg acccgcagct ccaaaagttt tccttcgaga agctgtcggc ggatttcatt
    22681 tcggtattcc tgagcggtca ttccgcttct caggacctcg gatagcagtt cctgcaccgt
    22741 ggtatcctgc atccgtgcaa ggcgagcaat agccttgtcg atttcctcgg cttccacccg
    22801 aattctcatt ttggccgctg cttgcgcttc aagctgatcg tcgatcatcc gttgaagcat
    22861 ctgccgcaac gtttcggatt cggcggcggc tctctgcgct tccgtgggta tcttcgaatg
    22921 aatctcgagg aggaatggtc gcgcacgctg ccgtaactcg ctcatgagaa tagggtgatc
    22981 cccgaccacg gcaacgaccc gttcgatcac ggccgcctgg gaagacgaag ccgagatcca
    23041 caaaataagc gagatgattc ccgccagtag gaaacgtcga tgcacaatca cagcggcctg
    23101 tgtctagcac acccggcctt tgcgcaagac aaaattggcc gtttcgctca aaagcgcagt
    23161 tccccgcgca cgtcttgctc atactgcgaa agcgtttcca tcgcttgatg cacggctttg
    23221 tcttgggagg tcggaacctg gacctgaaaa tgaacgtata gatctcccgg tggctgcccg
    23281 cgtcgagcca tcccctttcc ccgaagcctt gccgattggc cactttgcga acccgccggc
    23341 accttcatcg tcacggtgcc ctccggcgtt ggcacggtca ctcgcgcccc tcgatatgcc
    23401 tcggtgaccg tgatcggaag atcgacgtgc agatcatcgc cttgcatatg gaaatacgga
    23461 tgaggttcga catggagttc gaggagcaga tcaccgggag gcccaccaaa cggcgacgga
    23521 cttccttttc ctttcacgcg aactttgctt ccttgcttgg ccccaggagg aattctcacc
    23581 tggatcggtt cggcttggcc ttcaattcgg agcgatacgg tggaacctcg gacggcggat
    23641 acgaaatcga tcgtcagggg actttccagg tccgatcccc gcatggatgc cgtgcgggaa
    23701 gctcgcttcc gtcccgctcc cccaaaaagg ttgccgaata agtccccgat gttctgtccg
    23761 ccaagattcg caccgaataa gtcctccaag tcgacgcctt gaacgccgct tacccgaccg
    23821 cctccgccac cgcgcccttg cgtccactgt cggtacgctc gcgcttgttc cgcatcgaag
    23881 ttgtccgaca aggccgcatc tccaaattcg tcgtacaaag cccgacgctt tttgtcgctg
    23941 agcacttgat aggcctgatt gactcgcttg aatcgtgctt ccgcggaggc atcaccgggg
    24001 tttttgtccg ggtgtagctt cgatgccagt tttcggtagg cttgtttgat gtcgtttgca
    24061 ctggccgtgc gggttacacc gagttcttta taaaggtcgt tgctcatcgt atctcgacca
    24121 aggtatgtcg tggatcgccc cgaggcaaga tccggtcccc gcacgacacc tcaggtcaaa
    24181 cgcatcgcca actccgacac actctcgaac gtgttgaccc cctcgaaacg atgaggaagg
    24241 tacgcgagcg ggaccgactt gtcgcttgct cccatccctt cacgcaatcg caccaaagct
    24301 tcatgttgca ccagttctcg tcgcgcgcga cgaacccccg cggccaatgc ctcatccccc
    24361 aaacaggtcc cgctcagatc tcgtggtgcc agcaatgcct ctcgttcgga tgccgaaaac
    24421 atcggaggga tcacaccatt gacgatcaat tgcgccaccg gcagcccaag ctctttgtcg
    24481 agcgcttcgt acaattcgaa cgtttccgtc acgggcattt cctcgggcag tgtgaccaac
    24541 accacacctg cctgggtggg gtccgtgaac atcgaccacg ctttcatggc ttcacgatgc
    24601 agcagtccgg gaggtgccat atcgacgatc accttgggtc ctcgcaacat atccagggcg
    24661 tgtcctgtcg acggcgcatc gaaaagcacc acatcgaatc ggggctgtcc gtcttgtcgg
    24721 cgctcggtgg tgtgatacca ggccttgccc agcatcgccc attcgaaaag gcctggagtt
    24781 gctcgaaaaa acgaagcaac cagccggttt tcgaacaccg ctttgtacag aacctcgagc
    24841 cgcaacacca ttaggccata ctctcgaaga gcggcctccg gatgaatgtt cacagccatg
    24901 atgttggggc ggagttgcac gatctcctcc cccacggggg gcccctcgag caacgtggat
    24961 agccgatctt tggcttgaca cgtcgcgcac aaaacgcgct tgccttgcga agccaatacg
    25021 agcgcaagcg cggcggtggt cgtggttttg cctactccgc cttttccggt caaaaacagg
    25081 aaccgccgtc cttcaatggc cttggacacg ttcatgcgaa caatcgtttg cgtgcgaggt
    25141 ctacttgacc gacaacaatg ctttgagccg ttccaccttt tgctctttcg tcaagcacac
    25201 cgcatcgtga tccgttgcgg acatttccgc tctgagcgac gagctttcgt ctccaatacc
    25261 aaatacctga gggccgcttc gcgccatcac ccattcgata cgcacatcac ccggctcccc
    25321 ttccatcatt cgcacgccgc cttcgccaag atctttcaat tttgtcaccc cttccctttt
    25381 cgaaagggcc gtcaacagat ctttcgcttg gtccgcgtcg tcgcgggtga tggataccac
    25441 gcgataccgc tttccttcgt gttggtaata accaaaagca ccggcacctc caccctgcac
    25501 gccgaacacg tccttgggct cgaatcgaat tccgagagca atacggtttt ctgctggaag
    25561 gcgagtggcc gcggtcggaa gggtcgccgg agccggaagc tttgccgc
//
LOCUS       DAOSOR010000011        25430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_1954_length_25430_cov_5.049261, whole genome shotgun sequence.
ACCESSION   DAOSOR010000011 DAOSOR010000000
VERSION     DAOSOR010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 25430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25430
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1954_length_25430_cov_5.049261"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PK710_01195"
     CDS             <1..759
                     /locus_tag="PK710_01195"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HOT08346.1"
                     /translation="LTTVIEAVLEGLGTAREQFGIESNIIVCGIRNISPESSLEMAEL
                     AVAYKNRGVVGFDLAGAEYDHPAKHHKDAFQLVRDNNINTTIHAGEAYGPESIAQAIH
                     VCGAHRIGHAVGLRENGDLLHYVNDHRIPIECCPSSNVQTGAVRDLSAHPIKLYYNLG
                     LRVTVNTDNRLVTDTSVSKELWLCHTKMGFTVADIKSMIVAGFKSAFLPFHIKQQSLR
                     RLNKELKRFPDDGRILEEAFDIDRPRSSSDVVAS"
     gene            813..1202
                     /locus_tag="PK710_01200"
     CDS             813..1202
                     /locus_tag="PK710_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08347.1"
                     /translation="MVLLGGASECFDSLRNDGYSVRLDSERRVRLIATVVTEPLDIMA
                     ARSMRLCLATRGSVYVACPVSLLVSTWTVLRTAGLEPKKTVVFHPSEEACATDVVVVA
                     RPGKRGGLIVEVATSFAKRTDARSLPQ"
     gene            1422..5795
                     /locus_tag="PK710_01205"
     CDS             1422..5795
                     /locus_tag="PK710_01205"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOT08348.1"
                     /translation="MVKRRRQRVQVGRWIARVLCFLFAIIGIFPVLAGVLVRSEAVTN
                     WADQRVRALLIQDTGLDGLFRTTIHVWPPTIVISDIEVQASDDLGPALTIDRVSLRPQ
                     LFSLLQGKLNVGEIEIERPMVRLVIQKGKVVNLYWHMPTKDNEPSSSEDAFFSSLVIN
                     DARVDITVDGRRLRGKEIDLDITAAQGPVFDIALRTGRIQGTSIEPLSFTGYGAPEPV
                     QAHHEDVVCELDARLRVEPNGLQVRRFRAVGAADLDPNPDTRPSCQLSTDDPHRVELE
                     MRLVHAVLDDQGLKSVRGGGKARAPLRLVNRWIEFLPLQGWIGAEVQGEWRRGQSLPS
                     ISGAQLQGEGIALGIYRIASSLSLKARIDSDIVYVPEAHIGFADGLVAIHHAQVQPLA
                     KGIPLRAERLDIGGMKFPGLMRDLGVTEHTHVRMDFQEGTLSAVEGTIVPLRIDSNLM
                     THVRDFEVFDAAYDNPSRKHVLGISQGSVSAKFAVRPHAVEFQNSRVEFGKSSLNVFT
                     SLGFANEFRLVVSKGSRIDLEDINPLLDIPWKGKADVTTDITGVFDEPLIQGDVAIEG
                     FEFADMALGDLQRAKVRFQPMVMDITDVHGVKGYSAYHVPTMRVDFTGPAPVVADANL
                     RSSAFDIRDFFAIFHFDKDPRFKDIFGVAAANAQLHYEQGGPRDRCGGGWLGVQVDGS
                     IKQLELFEEKFDGGSFELDYEWFDREAQDLGVSADIRSIVLRKGKGTVLGQGTIRPGG
                     IIRGRAAVSHVPLSTIDTLGSLGAWLDARVSAVADVRGTLDRLEADIDVQVSPLQLGT
                     ATLPASHVFVKLTPIDPPVRVIGRTRCGLPIGAPFDPVAYAKDAPLGVFDVQGELFGG
                     QVIVQDVQLTRQTRKVATGTIVARALDLGKIAQVLPTMAASTDVHKGVLSGSLEMRYL
                     PLDDWRHADLSLVLAALEVQSNAGSVRLRQGTPPITLQQDDVIIPGILLDFHSPKGLS
                     GTFLAHGQVHKVSGTPQLDLHAQLTPTDLSALANIVPRIERARGVVQASIDVFGDWQA
                     PRYRGQASLQEGSLAITDIPMAIDGIDVLVRIDERQIQLVRANARVGGGRITAVGTLP
                     LEGFDFGTASATLTARDVHLAFLDGVKATIDADLTASWNARLAREMGNIPRVVGDVRL
                     LSFEYSRPFRMEADLPSLAEKARKTTFELYDPSQDVVDFEVRVHAPRPLWIRNNLADL
                     KLTLDSPVLTLSGSNQRVGLRGDLRVQPGSRVRLRANEFEVRDGLLRFDDATRLAPNL
                     DVTATTEFRRYSSGLEMSGAASGTEVSRAGGQWRIQLHAHGDTDNLRLDLTSEPALSQ
                     EDIVLLLTLGVTRAELDQMQASSLGETAALEALSTLTGADTMVRQSLPVIDDFRFGSA
                     YSSRSGRTEPTVTVGKRMTERVRANVTSGLSDTREVRSNLEWQLTGASSLLGSWDNVN
                     NVSNSALGNLGADVRFRISFE"
     gene            complement(5803..7314)
                     /locus_tag="PK710_01210"
     CDS             complement(5803..7314)
                     /locus_tag="PK710_01210"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT08349.1"
                     /translation="MNRIPSRICGALRGVALGMAISCTASMALGQESTPLRRLDEQRA
                     VRVAMANNPSFRTAVLDLQRANESVRFEDGRYPYTLQLEAGVTRSATPRVNADSVSIS
                     KSDSVDVGAQVSRTFATGTRASLRVEGYRDMTRAPVQSGPGYGTSARATVAQPLMRGA
                     GVEVGEQQLRIARINRTASELSKDRVASEVAREVLLAYWELWYAEAAVDIEGNARDLA
                     KRQRDEAQQRVNDGALAPVEVLPFETRLASLEESVISAQTDQRRRSLALARQMGTMEG
                     GETDLAAEIRQQPQVSLTPMRTAAVQQALSSSPEIKELEAQLRLAQEQIRTSGDSYRP
                     RLDVEGYVQVAGVGNQEVSPAFEQVATARATSVHVGLVYELPLDDARKSSERARDLLA
                     VQIAEQRLLAARQRIQTEVETLLEQERAARIRLEMAEKTAEIAEKQVAAATERFEIGV
                     GISVEVQQAEDELRKAKLRAVRARIDLVEAEVQRHHSTGQLLSRIADLLASSR"
     gene            complement(7307..8512)
                     /locus_tag="PK710_01215"
     CDS             complement(7307..8512)
                     /locus_tag="PK710_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT08350.1"
                     /translation="MITATIIMALREIRRNLMRSSLTMLGVVIGVGAVVALVSIGRGA
                     TESVTASIASMGHNMLTISPGAARRGGARTPSTPFKMEDVTAIQNDIRGLAAIAPTGS
                     RSLMLVYGNKNWRASVTGTNEDYVRVRNYAVAYGREMSAADVRSGRAVCMLGATTKKE
                     LFGSQDAIGESIRVDKISCEVIAVLKPKGEGGMGQDQDDLVIMPLLTFQRRIAGNRDV
                     STIYVSVDEGRPSSLVKDQITSLMREKRRTARGAEDDFNVRDMAELMATMESTTTALT
                     ALLGAIAAVSLIVGGIGIMNIMLVSVTERTREIGTRLAIGATGKEVLMQFLVEAVMLS
                     TLGGCIGVVLGVGGAGIASKYLNIPFVVVPEIIMVAFLFSATIGVVFGFLPARKAANL
                     NPIEALRHE"
     gene            complement(8509..9216)
                     /locus_tag="PK710_01220"
     CDS             complement(8509..9216)
                     /locus_tag="PK710_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT08351.1"
                     /translation="MTDQAKRALIHMLGVTKVYGKGEAEVRALAGIDLRVDEGEMIAI
                     MGQSGSGKSTCMNIVGCLDAPTSGTYLFRNVNVGELDNNQRALLRRHYIGFVFQGFNL
                     LARTSAQENVELPLVYRRVPAAQRREKAKAALQLVGLAGRESHKPNELSGGQQQRVAI
                     ARALASDPSLLLADEPTGNLDSARSAEVMELLVQLNRERGITVMVITHEPEMAEFCQR
                     TVEFRDGRILSDTRKPS"
     gene            complement(9213..10520)
                     /locus_tag="PK710_01225"
     CDS             complement(9213..10520)
                     /locus_tag="PK710_01225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT08352.1"
                     /translation="MEEPASQRPSLTTAQNLGLGKGQRWWRWVRWLIVLAAIAGIVVG
                     VLLWRAKASEAAKPHFETAAAEKGNILVTVSVTGKIQSVDAVEIGAEVSGKVDEVLVD
                     FNDAVKKGQELARINTEQLDARVKEVRAQSAVAAASLLSAKATAAEAKANVARMKSLA
                     DRGLVSQKDLEAAVATAARAEASVAQANAQQVVNAASLESALSARDKAIIRSPIDGLV
                     LSRSVEPGQTVAASLQAPVLFIVAKDLTTMVLKVDVDEADVGKVQKGQRATFTVDAYP
                     GKNFEASVKSLKNVPTSGQDVVTYEAELSVDNQERLLRPGMTATATIITSEKKDVLVV
                     PNTALRFTPPDVIASSTAATPRPGGALPGFGPPRFARPSGSAGGNRQKGAGQMGKVWV
                     LENGEPKAIRLRTGATDGTRTEVVGGELTEGMQVLIDIVTGAK"
     gene            11049..13259
                     /gene="buk"
                     /locus_tag="PK710_01230"
     CDS             11049..13259
                     /gene="buk"
                     /locus_tag="PK710_01230"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002834.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOT08353.1"
                     /translation="MFDIGASLDREILNVMKCRPTVLFIEPDDPRVLEAALHLPRVAK
                     PVFLAPRERVEETIKRQLPHLASAHIAFALGESAFCNLEQSTSLREELAHAAWQLPPE
                     QRVVNNLEEAYAWVSTPAGFGIMAAVSGHCDVVVGGARHEPRQYFRPLLKTLQAAPVL
                     CEVGVIILPDDHPQGLYPHNILILGDVGVNGTLTPDKLARIAVATCTVARDIIPEAEL
                     PEISGVMVSYSNHGGDEGPSPELVRKANRLVPEIIEEQYRNNERYRSISIQGEVKINV
                     ALSRRSERFYHGGVDQTDRYRGTNVLITPNLDTGNLLFHLFATRYPEAKTFSIVHGIR
                     FRGVDLPMDVESEDAVLAVKANLLRLHRYGQWVRTPRDTFFARPRILTINPGSTSTKV
                     AVFQGEEEIFSEELQHSASELEPFEGKRIVTQFEMRKQVVERTLQQHDVTIESIEAVS
                     ARGGLLRPIPHGTYSVNDAMRKDLLEGSLTDHASNLGALIAYALVEGTNKPAFIVDPV
                     VVDEMEERCRVTGIKSLRRKPISHALNQIATAHRYARDHETFYEYLNLIVCHMGGGIS
                     IGAHRKGRLVDVNNALDGEGPFTPQRSGSLPVGDLIRLCFSENVTKEQMLKLNKGRGG
                     LIDLLGTSDLKAIEQRYVQQDPEVVPVFEALGYQIGKSICSLVAAFDGQPVDRVLLTG
                     GMARSKPLVLLIDKACAALGCGMDVYPGESEMMALAQGALRVLEGREPAKEYEA"
     gene            13333..13965
                     /locus_tag="PK710_01235"
     CDS             13333..13965
                     /locus_tag="PK710_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08354.1"
                     /translation="MRLPWLISTQILVMSCCTGCALGEGSGEVVSQRLRLESCWDGPY
                     DMLPDFFAGVPYRDSFQIRIQRGGDQAEMSDGLAIMVDDVNQIRAQLGLPIAVGLSPE
                     VTPPGVPLTPDPNPPQVHMALYLHNTCREHVSTLHAIRGVITFEHLFNGDPNETNAGE
                     RLSSAAFDVTVADPRKQPAGGGPIPEQYLSRVTGWFQFYFERGQPGQPFP"
     gene            complement(14039..>14335)
                     /locus_tag="PK710_01240"
     CDS             complement(14039..>14335)
                     /locus_tag="PK710_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08355.1"
                     /translation="RKKVEEQKRLKEEAERKKAEEQKRLKEEAERKKAEEPKRSEKQA
                     KSKKTEEPKRLKEDETKPSPVPPVSENNKSMAFGVAVVAIVIVLVALMAWYFRN"
     assembly_gap    14336..14435
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14436..15665)
                     /locus_tag="PK710_01245"
     CDS             complement(<14436..15665)
                     /locus_tag="PK710_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08356.1"
                     /translation="MVERREAREPFSSRSDGIVAIPGIVCVTETTPTGGRVVHGGDAA
                     GWLKPAGILSWSHEVSVVARKDISDALCRDLRLLLPENRVLAIQFPRVHKAAKVAPLL
                     AVSSDDAKSLMERGIRVFTVGFDRISGGFFFFAQGGEKGTTSLQADLYQAIVPSLEAG
                     DSRTDIDACLDGIVDRILVEKSDGILSEMAVELAAALPRLAGAGQPVRALDDLEASMK
                     EIEQASVPFEKAAKEMISLLERSERNDETRVSIPLFGEAIREEQPVLVLQMMEGEGGK
                     TTFVQLSARFRWLVKGPIGEPAVAPTPAEEGQKEEAERKKAEEQKRLKEEAERKKAEE
                     QKRLKEEAERKKAEEQKRLKEEAERKKAEEQKRLKEEAERKKAEEQKRLKEEAERKKV
                     EEQKRLKEEAERKKVEEQ"
     gene            16011..17216
                     /locus_tag="PK710_01250"
     CDS             16011..17216
                     /locus_tag="PK710_01250"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HOT08357.1"
                     /translation="MRIDRARADGPFEHVANKAASHGRFLDLALTPETTYRYRLSYCL
                     TDTACHAPLMLEPLTTPKTRLPAFTIDIPPEGTQDDIVVFGIETLDPDALDLARMAGV
                     DRQGIIIWEYAREKPFLGPVTEVHWLEDRSLATGHSSFFVKLDLDGTELYRYEGNTAH
                     HDIDPLSNGRFIFLTFDAFADDLGGPILGDGIEIVQEGQSFPSWTWRARDHIPRTDRN
                     PVDWENFLFGIGHDWTHANALTMDEEANKIYVNIRNLNRIYCLDYPSGEVLWVMGDGG
                     DFGQGLFAHSHDPVFLSSHRVLVFDNGALRPEATHDYSRIIEIEFDAAARHAEIVWEY
                     RETPDFFSFAQGAIGIQPNGNLFVTDGINRRIFEVTRDKNKIWQMRLPPGTWTYKSIT
                     VPRSEFEDW"
     gene            17221..19140
                     /locus_tag="PK710_01255"
     CDS             17221..19140
                     /locus_tag="PK710_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08358.1"
                     /translation="MATSSPRMTHTTARRHSIPMALAFAVVLTIHLVLFVQVERQAPI
                     FNGEPLSFIDFDTHAVQMLRVTEALDRWGKHWAYDVQTLAGYPEGTVFNADNKGWELW
                     TYALWKLGLSRPTAFNLFVLFVHLLVPATVYLSSRLFRLEPWEALIATSIALGIWFFD
                     GLGRWSWYSGGISFAFVSYLFLLPLSLFYAHFRTGKRWLLWPLAALMSLGHLVHPSLF
                     VLLVVPMTGLYARHFRALRWTQHAAIVGVAVAVIGVNSWWLATAFRFAHYVTDHEPFF
                     VGRLFDIVLDAAGLTDNLTRTGLVGNRTGFRFLSLIAGFMGLVLWRKQRDDRFLPFAL
                     GIGFLFVMAYAGGYVWIFRQIQPYRNIVPTAFLASIPAAAFVGQLARNNIFKGFPRLM
                     WAPFGLLSFLSVLYLAKDVMYFLPFCLPAVPPLPTGEIVNITTYGFPPHRDFRHYPPE
                     KHLDDIVAWVRQHDDGQSRFLVEWWVLGEHLLSRTQAQVLGGFRERNMEHRAANLFHR
                     FPKGEIADDALARYLEQYAVRWVIVSLELPEFEKKPVLEQVATIGPHRIYRTRVPVSL
                     TQEEPAEVSASLNRIDVYGTNPNRDVTLRFHWFETLVCEPQCRIERTPLADDPVGFIR
                     VPAPHPTNFAIVNGY"
     gene            19130..20068
                     /locus_tag="PK710_01260"
     CDS             19130..20068
                     /locus_tag="PK710_01260"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOT08359.1"
                     /translation="MAIDARRTTDSRALLVLCARRRRWYGLFALLAALRILRDVVHYR
                     IRKREMANLASSLTVMVVMRLGWQDIVMRFGFAMALNVLVYLLNDIFDVRADHASGTR
                     DRDKIAFLQNNMKFGWAAASIPLGVMIGLGAMWNHELLWVLCVAGGSCLAYSARLKRL
                     AFLDLATIFVCATAGSMLAFPLDRALGWCLAGLLGCFAMCFQVVQMVRDHDEDASFGT
                     RTTAVVLGPQTMMRLQRVLLVLTAVYASLLVHRWVGVVLLLTVLLPFRAKEADRYWNH
                     LRLTLGVAWLAIVVFIGWTGMSYGWLTRLGYESLLW"
     gene            complement(20070..20843)
                     /locus_tag="PK710_01265"
     CDS             complement(20070..20843)
                     /locus_tag="PK710_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08360.1"
                     /translation="MSAKAVRPLWVGVFLSACAMGVGEERADPPNRPPRLDGSIGGEG
                     GYEWPEGGTAGYGAGGFGAGGFGAGGFGAGGFGAEGGEAGNGGQAAMAGAGGSTAACI
                     VGESKVIGNCGWCGTLQQDCDPQGVWGEEYCTGEGVCEPNSEETAKCGNCGTKKRTCL
                     GTCQWSDYGTCAGEGCKPGTTKDCLCSTNAAGTITSCCGQHVCDNECKWGSCGLKPTS
                     KCDWNAGHSYRCCGTRKWEYCLSNCDWSGNCASCDSCCK"
     gene            complement(20967..22154)
                     /gene="kbl"
                     /locus_tag="PK710_01270"
     CDS             complement(20967..22154)
                     /gene="kbl"
                     /locus_tag="PK710_01270"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOT08361.1"
                     /translation="MFEQAKPIYEKTLQEIQDAGLFKKERIITTPQGAQIAAGSGMVL
                     NFCANNYLGLSSHPEVVAAARDSLAGHGYGMSSVRFICGTQDIHKQLEATISQFFHTD
                     DTILYSSCFDANGGLFETLLSEDDAIISDALNHASIIDGIRLCKADRYRYANNDLEEL
                     EAHLRATQNKRLRLIATDGVFSMDGYLARLDEICDLAEKYKAMVMVDDSHATGFVGPT
                     GRGTPEHFGVLHRVDVMTGTLGKALGGASGGYTTGRQAIIDLLRQRSRPYLFSNTVAP
                     PIVAASLKVFEMLDKTSELRERLMHNTQVFRLGMKKAGFTIKEGIHPIVPVMLGDAKL
                     AVNMAAALLQEGIYVIGFSYPVVPKGEARIRVQLSAAHTSEHVQQALTAFEKVGKTMG
                     VIS"
     gene            complement(22264..23157)
                     /locus_tag="PK710_01275"
     CDS             complement(22264..23157)
                     /locus_tag="PK710_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08362.1"
                     /translation="MRIETKIGLVSTCIILIAAACGGRSGPEIWAIGEDAAPTGGWGG
                     GIVDGSPLGGQGGATGGSGGASGSSGAGGTGEGGTAGSTGGTSGSGGVTPDADVPDVS
                     IDAPQDASSDAKDFFDSIPGWNCVTCLEQRCKDKINACFNDPTCVSGVQCTVTQCLAG
                     GGNGGAGGGSAGGGGSGGGVDMACVLNCFDNDYGSAMTAVTMFMCITQSCGDECMGQG
                     GMGGAGGGSGSSLNGLGLPSGLFGYRDYPAPYRRHYIGTVRVPRPEEVAGSHPWLAEL
                     LSGRMPDPAPPCIVNQREKAR"
     gene            23283..24167
                     /gene="panB"
                     /locus_tag="PK710_01280"
     CDS             23283..24167
                     /gene="panB"
                     /locus_tag="PK710_01280"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOT08363.1"
                     /translation="MPQQDRKKITVPGLRARKTKGPKIAAVTAYDATIGCLLDEAGVD
                     VILVGDSLGMVFQGLPNTLPVTLDEICYHGRAVARAVTWAHLVGDMPFMSFQVSVEQA
                     VTNAGLMLKQGAFEAVKLEGGADYANHVRAIVRAGIPVMGHIGLTPQSVHAMGGFRVQ
                     GKGEDAAQRMIADAQALERAGAYAIVIEGVPADLASRITLAVDIPTIGIGASADCDGQ
                     ILVCYDYLGMVRNLKPKFVKQFAQVGDTIVQATRAYVDEVRAGTFPTATHCFRSSENN
                     APNEPAAHPKAYGPTSEH"
     gene            complement(24178..>25430)
                     /locus_tag="PK710_01285"
     CDS             complement(24178..>25430)
                     /locus_tag="PK710_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08364.1"
                     /translation="GGAKEGGGGWGWDGGPDGFVGPDGSSEFDPDADTCAWDSKKPER
                     AGVDIIFVIDNSHSMGDEIEKTLANINAFASKIANSGLDYQVIMLSAKGTNLTDVSED
                     VPNLKGSGLTGDMPLEVCVPPPLGVGPGGSDPCGDNAPRFHHLDNFPFGIASHNGMWL
                     AIGMYNKHYTWATDTGPDGGGWAKWARFNATKYFIMITDDDAYVPGTNEGDPSIIGNA
                     TEPYEVFDRLILKDPRYGPAGMFGTETNRKYVYNAVCGWTYPGGESMSDQEGGGCRTA
                     AGDPNPNFAMAPGIQHQKLAQLTGGIVESICRSDWSAVLGKLADNLVTAIGCEFDLPK
                     PSEGVLEPNKVLVQHTPEGGSPEPLTRVTDESKCDQYPNGWYYDDNVAPTRIVLCPGA
                     CSTIGAADTGQLDVLMGCQGPAPK"
BASE COUNT         5328 a         7677 c         6536 g         5789 t
ORIGIN      
        1 cttaccactg tcatcgaagc ggtgctcgaa gggttgggca cagcacggga acaattcgga
       61 atcgagagca atatcatcgt ttgcggaatc cgaaacatct ctcctgagtc ctccctcgag
      121 atggcagaac tcgccgtagc atacaaaaac cgtggtgtcg tcggattcga ccttgcggga
      181 gccgaatacg atcatcccgc caaacaccac aaagatgctt ttcaactcgt gcgcgacaac
      241 aacatcaata ccaccattca cgcgggcgag gcctacggac ccgaatccat tgcacaggcc
      301 atccatgtct gcggggccca tcgcatcgga catgccgttg gtcttcgcga aaacggcgac
      361 ctccttcact acgtcaacga tcaccgcatc cccatcgaat gctgcccctc gtccaacgta
      421 caaaccggtg ccgtgcgcga cctgtccgcc catcccatca agctctatta caatcttggc
      481 ctgcgcgtga ccgtcaacac cgacaatcgc ctcgtcaccg atacatccgt ttccaaagag
      541 ctttggcttt gccataccaa aatgggattc accgtcgccg acatcaaatc catgatcgtt
      601 gcaggtttca aatccgcttt cttacccttc cacatcaaac agcaaagcct tcgacgactg
      661 aacaaagaac tgaaaaggtt tccagacgac ggccgaattc tcgaggaagc tttcgacatc
      721 gaccgaccaa gatccagttc cgacgtggtg gcaagctgac ggcgcatcga cttcttctcg
      781 aattgcttcg cgatcaccct cgatcccagc cgatggtgtt gctcgggggt gccagcgaat
      841 gcttcgattc tttgcgcaac gatggttatt cggttcgttt ggattccgaa cggcgtgtgc
      901 gcctcatcgc caccgtagtc actgaacctc tcgacatcat ggcagcacgg tccatgcgtc
      961 tttgcctggc gacacgagga tcggtctacg tggcctgtcc ggtgtctctg ctggtatcca
     1021 cgtggaccgt tcttcgaacc gcgggtttgg agcccaagaa aaccgtggtt tttcatcctt
     1081 ccgaggaggc ttgtgcgacg gatgtggtcg tggtggcacg ccccggcaag cgaggcggat
     1141 tgattgtcga agtcgctacc tctttcgcga agcgaacgga cgctcgttct ttgccgcaat
     1201 gagcaggcct gtgcatggcg gacgaacacc agctcgatga taccagcccg acgatccacc
     1261 aaacgagggt tcgaggctcg agagccagtc attgctcatc gggcgagctt gcttgcctac
     1321 ccacgaaacc cgagtagtct ttctcattag gttcgcatgg ctgcacgtcg ttgctccact
     1381 ccttcgtccc atcagaaacg ctttgcgtgt tggtgaggct gatggtgaaa cgcaggcgcc
     1441 agcgtgttca agtgggtcga tggatcgccc gcgtgttgtg ttttttattc gcaattatcg
     1501 gtatttttcc agtacttgcg ggggtgctgg tccgctcgga agcggtcacg aactgggccg
     1561 atcaacgagt gagagccctg ttgatccagg acacgggtct cgatgggctt tttagaacca
     1621 ccattcacgt gtggccgccc accattgtca tttcagacat cgaggttcaa gcatcggatg
     1681 acttgggacc ggcgctgacg atcgaccgag tatcccttcg tccacaattg ttttctttgc
     1741 ttcaaggcaa gctgaacgtg ggcgaaatcg aaatcgagcg ccctatggtt cgtctggtca
     1801 tccaaaaggg gaaggtagtc aatctctatt ggcatatgcc tacgaaagac aacgagccat
     1861 catcgtccga agatgctttt ttctcttctt tggtgatcaa cgatgcgcgt gtggacatca
     1921 cggtcgatgg gcgcaggctg cggggaaaag agattgatct ggacattacc gcggcgcaag
     1981 ggcctgtgtt cgacatcgcg ttgcgtacgg gtcgaatcca aggcaccagc atagagcctc
     2041 tttcttttac gggatacggt gcgccggaac cggtgcaggc tcatcatgaa gacgtggtgt
     2101 gtgagttgga cgctcggttg cgtgtggagc ccaacggtct gcaagtgaga aggtttcgcg
     2161 cggtaggcgc ggcggatcta gatccgaatc ccgatacacg accttcatgc caactgtcca
     2221 ccgatgatcc gcatcgggtc gaactcgaga tgcgtttggt gcatgcggtt ctggatgatc
     2281 aagggctaaa gtcggtccgt ggtggaggca aagcacgggc gcctttacga ctggtcaacc
     2341 gttggattga gtttcttccc ttgcagggat ggattggcgc ggaagtacaa ggcgaatggc
     2401 gccgaggtca gtcgttgccc agtatttctg gcgctcaatt gcagggagaa ggaatcgccc
     2461 tggggatcta tcgcattgct agttctctat ctctcaaagc tagaatcgat tcggatatcg
     2521 tctatgtacc ggaagcccac attggtttcg cggatggtct ggtcgcaatt caccacgcac
     2581 aggtacagcc gttggcaaaa ggaatacctt tgcgagccga gcgcctggac atcggaggga
     2641 tgaagtttcc cgggctcatg cgcgaccttg gcgtcaccga acacacccac gtgcgcatgg
     2701 attttcaaga aggcactctt tcggcggtcg agggaaccat cgttccgcta cgcatcgaca
     2761 gcaatctcat gacccacgta cgggatttcg aagtcttcga tgcagcctat gacaatccat
     2821 cccgtaaaca cgtccttggc atatcccaag gtagtgtgag tgcaaagttt gccgttcgcc
     2881 ctcacgccgt ggaattccag aacagccgcg tggagttcgg aaaatcgagc ctcaacgttt
     2941 tcacgtcgct cggctttgcc aacgagtttc ggttggtggt gtcgaaaggc tcgcgcatcg
     3001 atctcgaaga tatcaacccg ttgctcgaca ttccgtggaa gggcaaagca gacgtaacga
     3061 cagacatcac aggtgtgttt gatgagcctt tgatacaggg cgatgtcgcc atcgaggggt
     3121 ttgagttcgc ggatatggcg ttaggggatc tgcagcgagc aaaggttcgt tttcaaccca
     3181 tggtcatgga catcaccgat gttcacggcg tgaaagggta cagtgcttat catgttccca
     3241 cgatgcgcgt ggacttcacg gggccggcgc ccgttgtcgc cgacgcgaac cttcgatctt
     3301 cggcttttga tattcgtgat tttttcgcca tcttccattt cgacaaagac ccacggttca
     3361 aggatatttt tggtgtcgcc gcggccaatg cgcagcttca ttacgaacaa ggaggaccgc
     3421 gcgatcggtg tggtggcggg tggctcggcg tgcaggtcga tggttccatc aaacagctcg
     3481 agttgttcga agagaaattc gatggtggct cgttcgagct cgattacgaa tggttcgacc
     3541 gtgaagcgca agacctgggg gtaagcgccg atatccgatc gatcgtattg agaaaaggca
     3601 aaggaactgt cctgggccag ggcaccattc gtcccggggg tatcattcgt ggtcgagcgg
     3661 cggtttccca tgtacccctg tccaccatcg ataccctggg cagcctcggc gcttggctcg
     3721 acgcgcgggt atcagccgtg gcagatgtgc gaggcacgct cgaccgattg gaagcggaca
     3781 tcgacgtgca agtgagtccg ttgcaactcg gaaccgctac gcttcccgcc tctcatgtat
     3841 ttgtcaagct gacaccaatc gatccacctg tgcgcgtcat cggacgcacg cgatgtgggc
     3901 ttcccattgg agcgcctttc gatccagtgg catatgcgaa agatgctccc cttggggtat
     3961 tcgatgtgca aggggagttg tttggcggcc aggtcatcgt gcaagacgtg caactgacgc
     4021 ggcaaacccg caaagtggcc acaggaacga tcgtggcccg tgctctcgat cttgggaaga
     4081 tcgcacaggt gttgcccaca atggcagcct ccacggacgt acacaagggc gtgttatccg
     4141 gttcgctcga gatgaggtac ttgcccttgg atgactggcg ccatgcggat ctgtcgctgg
     4201 tgctcgctgc tctcgaagta caatccaacg ctgggtccgt acggctccgt cagggcactc
     4261 ctcccatcac cttacagcaa gatgatgtga taattcccgg aattttgttg gatttccaca
     4321 gcccgaaagg attgtcggga accttcctcg ctcatgggca agtgcataaa gtcagcggca
     4381 cgccacagct cgacttgcac gcgcaactga cacccacgga cttatcagct ctcgccaaca
     4441 tcgtgccacg aatcgaacga gcgcgaggcg tcgttcaggc atccatcgat gttttcggag
     4501 actggcaggc cccccgctat cgaggacaag catcgttgca agaagggtcg ctcgcaatca
     4561 cggacatacc gatggccatc gacggtatcg atgtactggt tcgtatcgat gagcgacaga
     4621 ttcagttggt ccgtgccaat gcccgcgtgg gcggtggacg gatcaccgcg gtgggaaccc
     4681 ttcccctcga ggggttcgac ttcgggaccg catcggccac cctaaccgca cgggatgttc
     4741 atcttgcctt cctagatgga gtcaaggcaa ccatcgatgc ggacttgacc gcttcatgga
     4801 acgcaaggct tgcgcgagag atgggcaata tccctcgtgt ggtcggcgac gtgcgactgc
     4861 tatcctttga gtattcccgt ccatttcgca tggaagccga tcttccttct ttggccgaga
     4921 aagcacgaaa gacgactttc gaactctatg acccatcgca agacgtggtg gatttcgaag
     4981 tacgtgttca cgctccccga ccgttgtgga ttcggaacaa tctggcggac ctgaagctca
     5041 ccttggattc accggtgctc accttatcgg gctccaatca acgtgtgggg ttgcggggtg
     5101 acttgcgcgt tcagcctgga agccgcgtac gacttcgagc caacgagttc gaagtacgag
     5161 atggtctatt gcggtttgat gacgctacgc gtctggcacc gaatctggac gtgacggcga
     5221 caactgaatt tcgacgttat tccagtgggt tagagatgtc cggagcagcc tctgggacag
     5281 aggtatcgcg ggcggggggg caatggcgta tccaattaca tgcccatggc gatacggata
     5341 accttcgcct cgatctcacg agtgagcccg ccttatccca agaggatatc gttctgctgc
     5401 tgactttggg cgtcacacgc gctgaactcg accagatgca agcgtcgagt ctgggcgaaa
     5461 cagcagccct cgaagcgctg tctacactga cgggtgcgga taccatggtg cggcagtcac
     5521 tgccggtcat cgatgatttt cgttttggaa gcgcttattc gtccaggtct ggacggaccg
     5581 aaccgacggt tacggttgga aaacgaatga ccgaaagagt gcgcgccaac gtgactagcg
     5641 gtttgagtga tactcgagaa gtacgttcca acctcgaatg gcagctcacc ggagcttctt
     5701 cgttgttggg aagttgggat aacgtcaaca acgtatcgaa ctcggcgctt ggcaacttgg
     5761 gggccgatgt gcggtttcga ataagcttcg agtgacgctt gattagcgcg aagaggctag
     5821 aaggtcagca atacgcgaaa gcagttggcc ggtggaatga tgccgttgca cttccgcttc
     5881 gaccagatcg atccgagcgc gaacagcgcg gagcttggct tttcggagtt cgtcttccgc
     5941 ctgctggact tccacggaaa tcccgacgcc aatctcgaaa cgttccgtgg cggcggcgac
     6001 ctgtttctct gcgatttctg cggttttttc cgccatttcg agtcgaattc gagcggctcg
     6061 ctcttgctcc aacaacgtct cgacttccgt ttgaattcgt tgacgcgccg caagcagcct
     6121 ttgctccgct atctgcaccg cgagcaagtc ccgcgcccgc tcggaggact ttcttgcatc
     6181 atcgagcggc agctcataca ccaacccaac gtggacgctc gtggctcgtg ccgtggctac
     6241 ctgctcgaac gccggggata cctcttggtt tcccacaccc gctacttgca catatccctc
     6301 cacatcgagc cgagggcgat acgaatcacc cgacgttcga atctgctctt gggcgagacg
     6361 aagctgcgct tccagttcct tgatctccgg tgaactcgaa agagcttgct gcaccgcagc
     6421 cgttcgcatg ggcgtaagcg acacctgcgg ttgctgccgg atttcagccg ccagatccgt
     6481 ttcaccccct tccatcgttc ccatttggcg tgccaaggca agactacggc gtcgctgatc
     6541 cgtttgcgcg ctgatcacac tctcttcgag gctcgccaat ctcgtttcga aaggaagaac
     6601 ctccaccggc gccaacgcac cgtcattgac acgttgctgc gcctcgtcgc gctgacgttt
     6661 ggccaggtcc cgtgcgttgc cttcgatatc gacagccgct tccgcatacc agagttccca
     6721 ataggcaagc agcacttcgc gagccacttc actggcaacg cgatcttttg acagttccga
     6781 agccgttcgg ttgatcctgg caatccgaag ttgttgctcg ccgacttcaa cccccgcgcc
     6841 acgcatcaaa ggctgtgcca ctgtcgcccg cgcgctcgtg ccatacccag gtccgctttg
     6901 caccggcgct cgggtcatgt ctcgatatcc ctccacacgc agggatgctc gagtacccgt
     6961 ggcaaacgtc ctcgatactt gcgctcccac gtccacggaa tcgcttttcg aaatcgatac
     7021 ggaatccgcg ttcacacgcg gcgtggcaga acgtgtcact cccgcttcca attgcagggt
     7081 gtagggatat cttccgtcct cgaatcgaac gctctcgttg gcgcgttgca aatcgagaac
     7141 cgctgttcga aacgaagggt tgttcgccat ggcaacgcga acggcacgtt gctcatcgag
     7201 ccgacgcaac ggtgtggact cctgacccaa tgccatcgaa gcggtgcaag aaatagccat
     7261 gccaagtgcg acaccgcgta acgcaccgca tattcgagaa ggaatcctat tcatgacgaa
     7321 gcgcctcgat gggattgagg ttggcagctt tgcgtgctgg taaaaatcca aacaccacac
     7381 cgatagtggc cgaaaacagg aacgcaacca tgatgatctc cggcacaacc acaaatggaa
     7441 tattcaaata cttggaagca atgcccgctc cgcccacacc caacaccacg ccaatgcatc
     7501 cacccaacgt cgatagcatc accgcttcga ccaaaaactg catcaatacc tctttgccgg
     7561 ttgccccaat ggccaaacgc gtaccgattt cccgcgtcct ttccgtaaca gaaaccaaca
     7621 tgatattcat gatcccaatg ccccccacga tcaaactcac cgccgcaatc gctccaagca
     7681 acgccgtcag cgcggtggtg gtcgactcca tcgtcgccat caattccgcc atgtctcgaa
     7741 cgttgaagtc atcttccgct ccccgggccg tccgccgctt ctcgcgcatc aaggaggtga
     7801 tttgatcttt caccaacgaa ctcggacgcc cctcgtccac cgaaacgtag atcgtggata
     7861 catctctatt gcccgcgatt cgtcgttgaa acgtcagaag gggcatgatg accaaatcat
     7921 cctgatcctg ccccattccc ccctcgcctt tgggtttcaa aacggcaatg acttcgcaag
     7981 aaatcttgtc cacccgaatg gattcaccaa tggcgtcttg tgaaccaaac aattcttttt
     8041 tcgtggtcgc gccaagcatg caaaccgcgc ggcccgaacg aacatccgcg gcggacatct
     8101 cccgtccata cgcgaccgca taattccgaa cgcgaacata atcctcgttg gtccccgtca
     8161 cgctcgcacg ccagttcttg tttccataaa cgagcatgag gctgcgagat cccgtcggcg
     8221 cgatcgccgc tagtccacga atatcatttt gaatcgcggt cacatcttcc attttgaagg
     8281 gtgtggaggg agttcgcgcc cctccccttc gcgcggctcc aggcgagatg gtcagcatgt
     8341 tgtgccccat cgaggcaatg ctcgcggtca ccgattcggt ggcgcctcga ccaatactca
     8401 ccaacgccac caccgcacca acaccaatga ccacgccaag catcgtgagc gaggaacgca
     8461 tcaagttccg acgaatctct cgcaaagcca tgatgatcgt ggcggtaatc atgagggctt
     8521 cctcgtgtcc gataagattc gtccgtcgcg aaactcaacc gttctctgac aaaactcagc
     8581 catctccggc tcatgggtga tgaccatgac cgtgattcct cgctcgcggt tgagctgaac
     8641 gagtagttcc atcacctctg cacttcgcgc ggaatccaag ttccccgtgg gttcgtcggc
     8701 caacaataac gacgggtcgg aagcaagagc gcgagcgatg gctacccgtt gttgctgccc
     8761 tcccgacagt tcattgggct tgtgggattc ccttcccgca agcccaacca attgtaatgc
     8821 ggccttcgct ttttcacgac gttgagcggc gggaacacga cgatatacca aaggcaattc
     8881 gacgttttct tgcgcggacg tccttgccaa taggttgaat ccttgaaaca cgaaaccgat
     8941 gtaatgacgt cgtaacagcg ccctttggtt gttatccaat tcgcccacgt tgacattgcg
     9001 aaacagatag gtgcccgacg taggagcgtc gaggcatcct acgatgttca tgcaggtgga
     9061 ttttcccgaa ccgctctgcc ccatgatggc gatcatttcc ccttcatcga ctcgcaggtc
     9121 gatgccggca agagcgcgca cttccgcttc gcctttgcca tacaccttgg tcacgccaag
     9181 catgtggata agcgcgcgtt tcgcctgatc agtcactttg cacccgtaac gatgtcgatc
     9241 aatacctgca taccctctgt caactctccg cccaccacct ccgtacgggt gccatccgtt
     9301 gctcccgtcc gcaatcgaat ggctttgggc tcaccgtttt cgagcaccca gactttgccc
     9361 atttgacctg cgcctttttg ccgattgcct ccggccgatc cggacggacg tgcaaacctg
     9421 ggcggaccaa agcctggcaa cgcacctccc ggacgcggtg ttgccgccgt ggaagacgca
     9481 atcacatccg gtggcgtgaa ccgcaacgcc gtgttgggaa cgaccagcac gtcctttttc
     9541 tccgacgtta tgatcgtggc cgtggcggtc attccagggc gcaacaatcg ttcctggttg
     9601 tcgacgctca attccgcttc gtacgtcacc acgtcttgac cagaggtggg cacatttttc
     9661 agggatttga ccgatgcttc aaagtttttt ccaggatacg catcaaccgt gaacgtggcc
     9721 cgctgaccct tttgcacctt acccacatcc gcttcatcta cgtcgacctt aagcaccatc
     9781 gtggtcaaat ccttcgccac gatgaacaac acgggtgcct gcaacgaggc cgcaaccgtc
     9841 tggcccggct cgaccgaacg cgatagcacg agcccatcga tgggagagcg aatgatcgcc
     9901 ttgtctcgcg ccgacaacgc ggactcgagg gacgccgcgt tcaccacctg ctgcgcgttg
     9961 gcctgcgcta cggatgcctc cgccctcgca gccgttgcga ccgcagcctc cagatctttc
    10021 tgagacacaa gcccccgatc cgccaacgac ttcatgcgag ccacattcgc cttggcctcc
    10081 gccgccgtcg ctttcgcact cagcaacgat gccgccgcaa ccgcggattg cgctcgcact
    10141 tccttgaccc gagcgtcgag ctgctcggtg ttgatccggg ccaactcctg accctttttc
    10201 acggcatcgt tgaagtccac caggacttca tccacttttc ccgagacctc cgcaccgatc
    10261 tctaccgcgt cgacactctg aattttcccc gtgaccgaaa ccgtcaccaa aatattcccc
    10321 ttttccgcgg ccgccgtttc aaaatgcggt ttcgccgcct cactggcctt ggctctccac
    10381 aacaaaaccc ccaccacaat ccccgcaatc gcggcaagca caatcaacca gcgcacccag
    10441 cgccaccagc gttgtccttt tccaagtcca agattttgag cagtcgtcag tgaaggccgt
    10501 tggctggcag gctcttccat tcgtcgttct ccttagacgc caagcttccg caacgtggtg
    10561 ctttcaacgg tccgcgccaa cggacccgtc ggttagacca taacaaaatt cattctttct
    10621 ccaggttaga agcggccaca gcccccagga tcgcaattgg cacggatttt ctcggaaaat
    10681 ttccatctct ccgcaagcct tcggcacacg aaccgctccg gcccgtggag cagaaaacag
    10741 tcgaatcttc acacctcacc caaaacaccg ccgtaatcgc tttcccttcg ataacaccat
    10801 cacccatcac ggattcccga aactaaccgc atgccttttc agcatatcca ggaaagagac
    10861 aacagcctct tgcagctcgt tattaatcaa taaattccat tggttattag aatttcacgt
    10921 ctgcctactt cccaggcttc ggcgcagcgc gcaaaagcga ggatcgaaag gacgtgatat
    10981 cagacccacg tttgagaccg gccgtggtgg aaccggccat cgaaacgtct ctcgaaagga
    11041 cctcagccat gttcgacatt ggcgcatcgc tcgatcgtga aatcttgaac gtcatgaagt
    11101 gccggcccac ggtattgttc atcgaacccg acgatccgag ggtgttggaa gctgccctgc
    11161 acctgccaag ggttgcgaaa cccgtttttc tcgccccacg cgagcgcgtc gaagaaacca
    11221 tcaagcggca attgccacat ctagcctccg ctcatattgc gttcgccctc ggggaatccg
    11281 cgttttgcaa cctggagcaa tccacgagcc tgcgagaaga gttggcgcat gcggcatggc
    11341 aactcccgcc cgagcaacgc gtcgtcaaca acctcgagga agcctacgct tgggtgtcga
    11401 ctcccgcggg tttcggcatc atggctgccg tttccggcca ttgtgacgtg gtggtgggag
    11461 gcgctcgtca tgaaccacgg caatacttcc gacctttgct caaaaccctg caagcggccc
    11521 ctgtgctttg tgaagttggc gtcatcattt tgcccgacga tcatcctcaa ggcttgtatc
    11581 cgcacaacat cctcatttta ggcgatgttg gggtcaacgg aaccctgacg ccggacaagc
    11641 ttgcacggat tgcggtcgcc acttgcaccg tggcgcggga tatcattccc gaggccgagc
    11701 ttcccgaaat cagtggggtg atggttagct attcgaacca tggaggggat gaaggacctt
    11761 ccccagaact cgtgcgaaaa gccaaccgtc tggtgcccga aatcatcgag gagcaatatc
    11821 gaaacaacga gcgatatcgt tcgatttcga ttcaggggga ggtgaagatc aacgtggctc
    11881 tcagccgacg ttccgagcga ttctatcatg ggggtgtcga tcaaacggat cgctaccggg
    11941 gcaccaacgt gctgatcacg ccgaacctgg acacgggaaa cttgctgttt cacctgtttg
    12001 ccacacggta tcccgaagca aaaaccttca gcatcgtgca tggaattcga ttccgcggcg
    12061 tcgacctgcc gatggatgtc gaatcagaag acgccgtgct agcggtcaaa gcgaacttgc
    12121 ttcggctaca ccgttacgga caatgggtgc gaacacctcg agacacgttt tttgcccgac
    12181 cacgcatcct cacgatcaat cccggttcca cttccaccaa agtcgcggtc ttccaaggcg
    12241 aagaggagat tttttccgag gaactccaac attcggccag tgagttggag ccgttcgagg
    12301 gaaagcgcat cgtgacgcaa ttcgaaatgc gaaaacaagt cgtcgaacga accttgcaac
    12361 aacatgacgt aaccatcgaa tccatcgaag cggtcagtgc gcgcggtggc cttctgcgac
    12421 caatccctca cggcacctat tcggtcaacg acgccatgcg caaagatctg ctcgaaggct
    12481 ctctgaccga tcacgcctcg aaccttggcg cacttatcgc ctacgcgctc gtcgaaggga
    12541 caaacaaacc cgcctttatc gtcgaccccg tcgtcgtcga tgaaatggaa gaacgatgcc
    12601 gcgtgacggg tatcaagtca cttcgacgca aaccgatcag tcatgcgctc aaccaaatcg
    12661 caaccgctca tcgttatgcg cgcgatcacg aaacgtttta cgaatacctc aatctgatcg
    12721 tttgccacat gggcggaggg atatcgattg gggcacaccg caaaggacgt ctggtcgacg
    12781 tcaacaatgc gctcgacggg gaaggaccgt ttactccaca acgttcgggc tccttgccgg
    12841 taggcgatct catccgactt tgtttcagtg aaaatgtgac gaaagagcaa atgctgaagc
    12901 taaacaaggg aagaggaggg ctcatcgatc tgttgggcac gtcggatctg aaagcgatcg
    12961 aacaacgata tgttcagcag gatccggaag tcgttcccgt attcgaagcc ctcgggtatc
    13021 aaatcggcaa atcgatttgt tcgttggtcg cggccttcga tgggcaaccg gtggatcgcg
    13081 tgttgcttac gggagggatg gcacgttcga aacctctcgt tttactcata gataaggcat
    13141 gcgccgcact tggatgtggg atggacgtgt atcccgggga gagcgagatg atggccttgg
    13201 cgcaaggggc gttacgggtt ctggaaggac gggagccagc aaaggaatat gaagcataat
    13261 ctcggtcgtt ccttgggctt ttcgtgacgg gaacggatgc cttccggttg caaaccatgg
    13321 caagataata ccatgcgact gccttggctg atttcgaccc aaatccttgt gatgagctgc
    13381 tgcactggat gcgccttggg cgaaggttca ggagaggtgg ttagccaacg gctgcgtctc
    13441 gaatcctgct gggatggtcc ctacgatatg cttcctgatt tttttgcggg ggtcccttac
    13501 cgcgattcgt ttcagattcg catccaacgg ggcggggatc aggcggaaat gtccgatggt
    13561 ttggcgatca tggtcgatga tgtgaatcag attcgcgccc agttgggctt gccaatcgct
    13621 gtgggtctat cacctgaagt aactccgccc ggagtgcctc tcacgcccga tcccaaccct
    13681 ccccaagtgc atatggcgct gtacctgcac aatacatgcc gtgagcatgt ctccaccttg
    13741 catgcgatcc gaggggtcat cacattcgaa cacctgttca atggcgaccc gaacgaaacg
    13801 aatgccggcg aacgcctttc ttccgcggcc ttcgatgtca cggtggcaga tccacgcaaa
    13861 caacccgcgg ggggaggccc aattcctgaa caatacctgt cacgggtaac gggttggttc
    13921 caattctatt tcgaacgagg tcaaccggga caaccgttcc cataacctcc acacgccgat
    13981 accccccgac cgaagcatac gcatccgata agcatgatcg tcgtgcatca ttttccgatt
    14041 agtttcggaa ataccatgcc atcagcgcca ccaacacaat cacgatggca accacggcca
    14101 caccaaaagc catactcttg ttgttctcag aaacaggcgg aacaggggat ggcttggttt
    14161 cgtcttcttt caaccgcttt ggctcttcgg tcttcttgct cttcgcctgt ttttcagagc
    14221 gctttggctc ttcggccttc ttgcgttccg cctcttcttt cagccgtttt tgctcttcgg
    14281 ccttcttgcg ttccgcctct tctttcagcc gtttttgctc ttcgaccttc ttgcgnnnnn
    14341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttgct cttcgacctt cttgcgttcc
    14461 gcctcttctt tcagccgttt ttgctcttcg accttcttgc gttccgcctc ttctttcagc
    14521 cgtttttgct cttcggcctt cttgcgttcc gcctcttctt tcagccgttt ttgctcttcg
    14581 gccttcttgc gttccgcctc ttctttcagc cgtttttgct cttcggcctt cttgcgttcc
    14641 gcctcttctt tcagccgttt ttgctcttcg gccttcttgc gttccgcctc ttctttcagc
    14701 cgtttttgct cttcggcctt cttgcgttcc gcctcttctt tttgaccttc ctccgcagga
    14761 gtcggcgcta ccgctggctc tccgataggc cctttcacaa gccaccgaaa gcgcgccgat
    14821 aactgaacaa aggtcgtttt tcccccctcc ccctccatca tctgaagcac aaggacaggt
    14881 tgctcctcgc gtatggcttc cccaaatagg ggaatggaaa cacgagtttc gtcattgcgc
    14941 tcgcttcgct ccaaaaggga aatcatttcc ttggcagcct tttcgaacgg cacggaagcc
    15001 tgctcgatct ctttcatcga ggcttcgagg tcatccaagg cgcggacggg ttgccctgct
    15061 ccagccaatc gtggcaaggc cgcggcaagc tcgacagcca tttccgacag gatcccatcg
    15121 gatttttcga ccaaaattcg gtccacgatc ccgtccaaac acgcatcgat atccgtacgg
    15181 gaatcgcccg cctcgaggga gggaacgatg gcctggtaca agtctgcctg gaggctggtg
    15241 gtgccttttt cccctccctg cgcaaaaaag aagaaccccc cggagattct atcgaatccc
    15301 acggtaaata cgcggatgcc ccgttccatc aagcttttcg catcgtcaga agacaccgcc
    15361 aacaagggag cgactttggc agccttatga acccgaggaa attggatcgc caatacccgg
    15421 ttttccggca gcaacaaccg aaggtctcga cacaaagcgt cggaaatgtc tttccgcgca
    15481 acaaccgata cctcgtgcga ccaagacagg atccccgcgg gtttcagcca tcccgcggca
    15541 tcacctccgt gaaccacccg ccctcccgtg ggagtggttt ccgtcacgca tacgatgcca
    15601 gggattgcaa caatgccgtc gcttcttgaa gaaaacggtt cgcgagcttc tcgtcgttcg
    15661 accacgaatc cccttttagc acgccttttg ttgaaagcgc ggaaaacaca tgccctgttc
    15721 caacaggctg cgcgatcgtg ccataattcg gggctgctaa cccttgtctt cccttggatc
    15781 gggctggtgt ccgtttgtct tcccccgtat gatcccgcgg gctttttgtc atggttcatc
    15841 gctatttttc gctcgtttca gccatagtcc tttcgttcgg atccggatgc gggccggaag
    15901 gcacggcaag cacgcaagct ccgcacaccc cgagcctcgt gctacgccaa gcatcagccc
    15961 tatccgctcg caacatcaaa atctcttggg aaccaatacc gtcaggacgt gtgcgtatcg
    16021 atcgtgcacg agcagatggc cctttcgagc atgtcgcaaa caaagccgct tcccatggcc
    16081 gcttcctcga tctcgcccta acccctgaga caacctaccg ctatcgtctt tcctattgcc
    16141 tcaccgacac cgcttgccac gcgccgctta tgctcgaacc cctgacaacc cccaaaacga
    16201 gacttcccgc cttcaccatc gatatccctc ccgagggaac ccaagacgat atcgtcgtat
    16261 tcggaatcga aacgctggat ccggatgccc tagacctggc tcgcatggct ggcgtcgacc
    16321 gtcagggaat catcatctgg gaatacgccc gtgaaaaacc ttttctgggc cccgtgacag
    16381 aagtgcattg gctcgaggat cgcagccttg caactggaca cagctccttt ttcgtgaaac
    16441 tcgatctgga tggcacggag ctgtatcgtt acgaaggcaa cacggcgcat catgacatcg
    16501 atccgttatc gaacggacga ttcatttttt tgacattcga cgcttttgcc gacgatctgg
    16561 gcggtccgat attgggggat ggaatcgaga tcgtgcagga aggacagtcc tttccttcct
    16621 ggacttggcg cgcgcgcgac catatccccc gcacggatcg aaatcccgtc gactgggaaa
    16681 atttcctatt tggcatcggg catgattgga cacatgccaa cgctctgacc atggatgagg
    16741 aagcgaataa gatttacgta aatattcgaa atctaaacag aatatactgt ttggactacc
    16801 cgagtggcga ggttctttgg gtcatgggcg atgggggaga tttcggacaa ggattgtttg
    16861 cccatagcca cgatcccgtt tttctttctt cccatcgcgt gctcgttttc gacaacggcg
    16921 cgctccgccc cgaagcaacc catgactact cacgcatcat cgagatcgag ttcgacgccg
    16981 cggcgcgaca cgccgaaatc gtctgggaat accgtgagac ccccgacttt ttttcctttg
    17041 cccaaggggc gatcggcatc caaccgaacg gaaatctttt tgtgacggat ggtatcaatc
    17101 gacggatttt cgaggtgacg cgagacaaga acaaaatctg gcaaatgcgt ttaccccccg
    17161 gaacatggac ctacaaatcc atcaccgtcc cgcgcagcga gttcgaggat tggtaatccc
    17221 atggccacct cgagtccacg gatgacacac accacggcgc gtcgtcactc gattccgatg
    17281 gctcttgcgt ttgcggtcgt tttaacgatt cacctcgttt tgttcgtgca agtggagcga
    17341 caagctccga tattcaatgg tgaaccgctt tctttcatcg atttcgatac ccacgcggtg
    17401 cagatgttgc gtgtcaccga agcgctcgat cgatggggaa aacactgggc atacgatgtg
    17461 caaaccctgg cggggtatcc tgaaggaact gttttcaatg cggataacaa agggtgggaa
    17521 ctatggacct atgccttgtg gaaactcggc ctttctcgac ccaccgcttt caacttattc
    17581 gtcctattcg ttcatcttct cgtcccggca accgtgtacc tatcgtcgag actgttccgc
    17641 ctcgaacctt gggaggcctt gattgccacg tcgattgcct tgggcatttg gtttttcgat
    17701 ggcctgggcc ggtggtcgtg gtactcggga ggcatttcct ttgccttcgt cagttatcta
    17761 ttccttcttc ctttatcctt attttatgct cattttcgta cgggaaagcg ttggttgctg
    17821 tggccgttgg ccgcgctgat gagcctcggg catctcgtgc atccttccct tttcgtgctg
    17881 ctcgtagttc ccatgaccgg gctttatgct cgccattttc gagcgttgcg ttggacacaa
    17941 cacgccgcca tcgtcggcgt tgcggtggcg gtcattggtg tcaatagctg gtggctcgcc
    18001 acagcgtttc gtttcgcgca ttacgtcacg gaccacgaac cttttttcgt gggacggtta
    18061 ttcgatatcg tattggatgc ggcaggactc accgacaatc tcacacgcac gggcctggtg
    18121 ggaaatcgca cgggctttcg atttctgtct ttgatcgcgg gattcatggg cctcgtgctg
    18181 tggcgaaagc aacgcgatga tcgtttcctt ccctttgcgt tgggaatcgg atttttgttc
    18241 gtgatggctt atgcgggcgg gtatgtatgg atctttcgtc aaattcagcc ttaccgcaac
    18301 atcgtcccca ctgcgttttt agccagtatt cccgctgccg cattcgtggg tcagctagca
    18361 cgaaacaaca ttttcaaagg atttccacgt ttgatgtggg cgccctttgg cttgctgtcg
    18421 tttctatccg tgctgtacct agccaaggat gtgatgtact ttttaccttt ctgcttaccc
    18481 gctgtgcccc cccttcccac gggcgaaatc gtaaacatca caacatacgg tttcccgcct
    18541 catcgagatt ttcgtcatta tcctccagaa aaacacctgg acgatatcgt cgcctgggtc
    18601 cgtcaacacg atgatggcca gagccgattc ctcgtggaat ggtgggtgct gggagaacat
    18661 cttctgtccc ggacccaagc tcaagtcttg ggaggttttc gcgaacgaaa catggagcac
    18721 cgggcagcca atctttttca tcgctttccc aaaggcgaga tcgccgacga tgccttggcg
    18781 cggtatctgg aacaatatgc cgttcgttgg gtcatcgtga gtctcgagct accggagttc
    18841 gaaaaaaaac cggtgctcga acaagtggca accattggtc ctcatcgcat ttatcgcacg
    18901 cgggtgcccg tctctttgac ccaggaagag ccggcagagg ttagcgcgtc gctcaatcgg
    18961 atcgatgtgt acggcacaaa tcccaaccgg gatgtgacgc ttcgattcca ttggttcgaa
    19021 actctggttt gtgaacccca atgccgaatc gaacgaacac ctctggcaga cgacccagtc
    19081 ggattcatcc gtgtgccagc tcctcatccc accaattttg ccatcgtcaa tggctattga
    19141 tgcgcgaagg accacagact ctcgtgcttt gctcgtactt tgcgcgcgtc gtcgacgatg
    19201 gtacggtctg ttcgctttgc tagctgctct tcgcatcctt cgcgacgtcg tccactatcg
    19261 cattcgtaaa cgcgagatgg cgaatttggc atcatcactc accgtgatgg ttgtgatgcg
    19321 attgggctgg caagacattg tgatgcgctt tgggttcgcg atggcgctga acgtattggt
    19381 atatctcctc aacgatattt tcgacgttcg ggcggaccat gcctcgggga cccgagatcg
    19441 cgataagata gcgtttttac agaataatat gaagttcggc tgggctgcgg cttcgattcc
    19501 cttgggcgtg atgatcgggc ttggcgcgat gtggaatcat gagcttttgt gggtgctatg
    19561 cgtggccggg ggctcatgcc tcgcttattc agctcgtctg aaacgactag cttttcttga
    19621 tcttgccacg atcttcgtat gcgcaaccgc cggctccatg cttgccttcc ctctcgatcg
    19681 agcccttgga tggtgtcttg ccggactgct gggatgcttt gcgatgtgtt ttcaagtcgt
    19741 gcagatggtt cgtgaccacg acgaagacgc ttcttttgga acgcgcacca ccgcggtcgt
    19801 gttgggtccc cagaccatga tgcgactgca acgagttctt ctcgttctca ccgcagtgta
    19861 tgcgtcgctc ctggttcatc gctgggttgg agttgtacta ttgctgaccg tgctgctccc
    19921 gtttcgggcg aaagaagcgg atcgctactg gaaccatctg cgactcaccc tgggagtggc
    19981 atggctggcc atcgttgttt ttattggctg gacggggatg tcgtatggct ggctgactcg
    20041 tttgggttac gaatccttgc tctggtaagt catttacagc agctatcaca agaggcgcaa
    20101 ttgccagacc aatcgcaatt cgacaggcaa tactcccact tcctggtgcc acagcatcga
    20161 taggaatgtc ctgcattcca atcgcatttg ctggtaggtt tcaatccgca ggatccccat
    20221 ttgcattcgt tgtcgcaaac atgctgaccg caacaggaag tgatcgttcc ggccgcattc
    20281 gtgctgcaca gacagtcctt cgtggtccca ggcttgcaac cttcaccggc acaagtaccg
    20341 taatccgacc attggcatgt ccccaaacag gttcgctttt tggttccaca attgccgcat
    20401 ttcgctgttt cttcggagtt cggttcgcac actccctccc ccgtgcaata ctcttcgccc
    20461 caaacacctt gggggtcaca gtcctgctgt aaggttccac accaaccgca gttgccaatc
    20521 accttcgact caccaacgat gcaagctgcc gtgctaccgc cagcgcccgc catcgctgcc
    20581 tgcccgccat tgccggcttc tccaccctca gccccaaagc caccagcccc aaagccacca
    20641 gcaccaaagc caccagcacc aaatcctcca gccccataac ccgcggtgcc cccctcgggc
    20701 cattcgtatc caccttcacc gcctatactc ccatccaatc gaggcggtct attcggagga
    20761 tccgcgcgtt cttctcccac gcccatcgca caagcagaca gaaaaactcc aacccataat
    20821 ggtctgactg ctttcgccga cacgttacac ctctgccgta gtttatcacc ccacggcgag
    20881 gattccatct gttcgaaagg atccaaccca aatggcgata cggaagggtt cgggaattgc
    20941 actccaaacg gacgcggcaa caagggtcag gagatgacac ccattgtttt tcctactttt
    21001 tcaaacgccg taagcgcctg ctggacgtgt tcgctcgtat gagccgcgga tagttgcacg
    21061 cgaattcgag cttccccttt cggtacgacc ggataagaaa atccgatgac gtaaatccct
    21121 tcttgcagca acgccgccgc catgttcacg gcaagcttcg catcccccaa catgaccggc
    21181 acgatcggat ggatgccttc tttgatcgta aatcccgcct tcttcatccc caagcgaaat
    21241 acctgcgtat tgtgcatcaa acgctcgcga agctcactcg ttttgtcgag catctcgaat
    21301 actttgagcg atgccgcgac aatgggcgga gccaccgtgt tggaaaatag ataaggacgc
    21361 gaccgctgcc gcagcagatc gatgatcgct tggcgcccgg tcgtataccc tccactggct
    21421 cctcccaacg ctttgccaag cgttcccgtc atgacatcga cccggtgcaa cacaccaaaa
    21481 tgctcgggag ttccgcgacc cgtcggaccc acaaacccgg tcgcatgcga atcatccacc
    21541 atcaccatcg ccttgtactt ttccgcgaga tcgcaaatct catcaaggcg agcgagatat
    21601 ccatccatcg agaacacgcc atcggtcgcg atcaaacgca agcgcttgtt ctgcgtggct
    21661 cgaagatgcg cttcgagttc ctccaagtca ttgttggcgt aacgatatcg atcggctttg
    21721 cacaaacgaa tgccatcgat gatcgacgca tggttgagcg catcgctgat gatggcgtcg
    21781 tcttcggaga gcaacgtttc gaaaagaccg ccattggcat cgaaacaaga cgaatacagg
    21841 atggtgtcat ccgtatggaa aaactgcgag atggtggctt cgagttgttt gtgaatgtct
    21901 tgggttccgc agatgaagcg cacgctgctc atcccataac catggcccgc gagagaatca
    21961 cgggccgcgg caaccacctc gggatgagac gacaagccga ggtagttgtt ggcgcaaaag
    22021 ttgagcacca tgccagaacc ggccgcgatt tgcgcgccct gcggcgtggt aatgatgcgc
    22081 tcttttttga aaagccccgc atcttgaatc tcttgaagcg ttttttcgta aatgggtttc
    22141 gcttgctcga acatggcgca ggcatacccc tgcgcgagaa cgaagggaac ccccgaagta
    22201 tcctcgattc cgcccgtcta gccgagcctt aggctcgcgg ccgatggctc ctcggcaccg
    22261 gctttacctg gctttttcac gttggttcac gatgcatggc ggggcaggat ccggcatacg
    22321 gccagaaaga agttccgcca gccaaggatg cgaaccggct acctcttccg ggcgaggaac
    22381 ccgcaccgtc ccaatataat ggcggcgata gggtgcggga taatcccgat aaccgaagag
    22441 gccagaaggc aacccaagac cattgagcga cgaaccggaa ccgcctccgg cgcctcccat
    22501 gccgccttgc cccatgcatt cgtctccgca agactgggtg atgcacatga acatagttac
    22561 cgcggtcatg gcagagccgt aatcattgtc gaaacaattc aatacgcacg ccatatcgac
    22621 accgccccca ctacctccac ccccggcgct tccccctccc gcaccaccgt tgcctccacc
    22681 cgcaagacat tgcgtcaccg tacactgcac accggaaaca cacgtgggat cgttgaaaca
    22741 cgcgttgatt ttgtctttgc acctctgttc gagacacgtc acgcaattcc atccgggaat
    22801 cgagtcgaaa aagtccttcg catccgaaga agcgtcttga ggcgcatcga tcgatacgtc
    22861 aggcacgtcc gcgtccggcg tgactccacc cgaaccggaa gtaccgccgg tagaacccgc
    22921 ggtccctcct tcacccgtgc cccctgcccc gctagaacca ctcgctccac ccgatcctcc
    22981 cgtggctcct ccttgtcctc ccaagggaga accatcgacg attccaccac cccatcctcc
    23041 cgtcggtgcc gcatcctcgc caatggccca aatctctggc ccactgcgcc caccacaggc
    23101 tgctgcaatg agaatgatgc atgtgctgac cagaccaatt ttggtttcga tgcgcatgaa
    23161 cggtgtcctt cgtggttatc gtagtctatc agaacacacg ataaaccgct cgtgggtgga
    23221 tttcgttctc gtactatgtc agatgcgggg tcgggccgcc cggcccaggc aggagaaatc
    23281 acgtgcccca acaagatcgg aaaaaaatca ccgtccccgg actgcgcgcg cgaaaaacga
    23341 agggccccaa aattgccgct gtcaccgcct acgacgccac catcggctgc ctcctcgatg
    23401 aagcaggcgt cgacgtcatc ctcgtaggag attccctggg catggtcttt caagggctcc
    23461 ccaataccct gcccgtcacc ctcgacgaaa tctgctacca cggacgcgcc gtcgcacgag
    23521 ctgtaacttg ggcccacctg gtgggcgaca tgccattcat gtcctttcag gtttccgtgg
    23581 aacaagccgt aaccaacgct gggctgatgc tcaaacaagg agccttcgaa gccgttaagc
    23641 tcgaaggagg agccgattat gccaaccatg tgcgcgcgat cgtccgcgct ggcatccccg
    23701 tcatgggaca tattggcctc acaccacagt ccgtccacgc catgggaggc tttcgtgtcc
    23761 agggaaaagg cgaagatgcc gcgcaacgta tgatcgccga cgcccaagct ctcgaacgag
    23821 ccggcgccta tgccatcgtc atcgaaggcg tccccgccga cttggccagc cgcatcaccc
    23881 tcgcggtcga tatacccacc atcggaatcg gtgccagcgc tgattgcgat ggccaaatcc
    23941 tcgtatgtta cgattactta ggcatggttc ggaacctcaa gcccaagttc gtcaaacagt
    24001 ttgctcaagt cggcgacacc atcgtgcagg ctacccgagc ctacgtcgac gaagtgcgcg
    24061 cgggcacctt ccccacagct acccattgct tccgatcatc tgaaaataac gcgcccaatg
    24121 agcccgcggc acaccccaaa gcctacgggc ccacctcaga acattgattg acttgattta
    24181 cttgggcgcc ggtccctgac aacccatcaa tacgtcaagt tgccccgtat ccgcggctcc
    24241 aatcgtagaa cacgcacctg gacataaaac gattctcgtg ggagccacgt tgtcgtcgta
    24301 ataccagccg tttgggtatt gatcgcattt gctctcatcg gtcacccgcg tgagaggctc
    24361 gggagaacca ccttcgggcg tgtgttgcac gagaaccttg ttgggctcga gaacaccctc
    24421 gctcggtttg ggtaagtcga attcacaccc gatggccgtc accagattat ccgccagttt
    24481 tccaagcacc gcggaccaat cggaacgaca gatggattcc acgatgccac cggtaagctg
    24541 agcaagcttc tgatgctgaa tcccaggagc catcgcgaag ttgggattcg gatcgcccgc
    24601 ggccgtccga caacctcccc cctcctgatc gctcatcgat tcgccacctg gatacgtcca
    24661 tccacaaacc gcgttgtaca cgtacttgcg attcgtttcc gttccgaaca tccccgccgg
    24721 accataacga gggtctttca aaatcaaacg atcgaacacc tcataaggtt ccgtggcatt
    24781 gccaatgatc gacggatcgc cttcattcgt gccgggaaca taagcatcgt catcggtgat
    24841 catgatgaaa tacttggttg cattgaaacg cgcccacttg gcccatcccc cgccatcggg
    24901 acccgtatcg gtggcccacg tataatgctt gttatacatg ccaatggcga gccacatccc
    24961 gttatgactc gcaatcccaa aggggaaatt gtccagatgg tgaaaacgtg gcgcattgtc
    25021 cccgcaagga tcgcttcccc ctggccctac gccgagcggc ggcggaacgc atacctccaa
    25081 cggcatgtcc cccgtcaacc ccgaaccctt gagattggga acgtcctcac tcacatccgt
    25141 cagattcgtt cccttcgcac tgagcatgat gacttgataa tccaggccgg agttcgcgat
    25201 cttgctggca aaagcgttga tattggcaag cgtcttctca atctcgtcgc ccatcgaatg
    25261 cgaattgtcg atcacgaaaa tgatgtcgac acccgcccgc tcaggctttt tcgaatccca
    25321 cgcgcacgta tccgcatcag ggtcgaattc actgctgcca tcagggccca caaacccatc
    25381 cggaccgccg tcccaacccc accccccccc cccctctttg gctccccccc
//
LOCUS       DAOSOR010000012        24913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_2010_length_24913_cov_4.523212, whole genome shotgun sequence.
ACCESSION   DAOSOR010000012 DAOSOR010000000
VERSION     DAOSOR010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 24913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24913
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2010_length_24913_cov_4.523212"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="PK710_01290"
     CDS             complement(<1..1111)
                     /locus_tag="PK710_01290"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08365.1"
                     /translation="MTGAAGKTIVNPVPYELARRLLASHGMTEQQAHAALAMHVKHSV
                     SLVRAFRDSGVSLEAIDRECRRSNAPTLHHIMPAMDVFYQLPEGLCEGLLAVPVRRDP
                     MTGTIDVATPDPFGDHIRQEFAHHLQAPIRIVYSPLLVVENALEQIKALRKNRERPSA
                     SDIPIPLVRRSRAPKMGNQPRTKDIYNPPEPIVLSPGSLPATRLQTSDWQDNRIDPPT
                     VESFHIAIPPSAALPTIEGVSAPFQLEESVAQIEAVLEELQQAATRDQVVRAALRAME
                     AVAHRVGIFAVRNDVFSGWACTPSFASVDALKTIRVDRHRDTVLSHAALHGWYFGTVP
                     TDAAHQWLADIAPPAGSEIAIVAVRILGRAAMLIVA"
     gene            complement(1108..1407)
                     /locus_tag="PK710_01295"
     CDS             complement(1108..1407)
                     /locus_tag="PK710_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOT08366.1"
                     /translation="MVNLSQFLERGLPILILSVAAVGAPALMLSQEGLPRLRALQAEK
                     ARVEAENAELSEKIRQLRVEVEMLKRDPRAVERIARDGLDLVRNTEEVFLFPSRP"
     gene            1592..6913
                     /locus_tag="PK710_01300"
     CDS             1592..6913
                     /locus_tag="PK710_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT08367.1"
                     /translation="MDKDTLRSELERLFDLEELMDVSRDVLGISPDEVGHTASKALFA
                     KALVDRCVVTERVEALLDSLITLRPTVDRKALQKVSSSLVAEHELPAGSSFGPFVVRR
                     KVGSGPSGSVYLVKRDDRELTLKVLHPLMGRDRGSFARFAAATRLFSRVSHEGLPTAM
                     TLGNDEGLDFVAYDYIDGQSLASRTARSGPMHINEARSLLQKLLDVLVALHDQRLVHG
                     NIKLSNILVARGSEPGKQKLVLLDPAFDRLRLLRSSSSSDDVCVVLSHARSVSPEQLR
                     GVGADERSDVYSFGAVMYEILTGKPLFSAGSALEYAIATWTQDPEPPIQAAPRGWVAK
                     EVSDFIMSLLAREPSLRPKDARAVLERFEMLGRVATQDDSTNKISDEELSERIDALVI
                     SPDDEAAIAALDEAAERGGDVAKIAQALVMAADQIETTDSDGVELRKSLLFRSARLYE
                     SKLKDLPEAEKLYVWISELDPADEIADAALEEVRRQLGKTEEVVEMLLARIERAADSE
                     EKATAMAEIGRLYASELEDASQAVVAYAQAFVESPKHVEFADAIERIAGSDVNVLGEA
                     MAILAESTQTDIPIENKNLLFLRLGHWYGKRLGRYDAALPCYQAVIATDPVNDGALEG
                     MAALFKANQQYMELGQVLMRRAEAASTPARSREFRLDAAELLETRLGEPAKAKELYEQ
                     VLAEDPSHDRATEALLGIYEKEDNAASLVKILTNKAERLSGSQAADIWVRAGDVYETK
                     LKDMTNAIRCFESAVQQDETNQRALDALDRLHTQSRHFKELLATIERKINIATTPRQK
                     IVHLERISKLHAEEFLDHVAAAQSLEKILEIDPAHSATLEALAHHYRALDRWSDVANV
                     YERDLNLQTDKAKRLDVLLMLARVVAERLSDPARAMKVYEQALEIEPGHAGALEALAA
                     LRQQSGDSQAALQAIEALAEKASSPNEKAEQYIRAAKLLQERDDVEGAMERYRLALDA
                     NPRAFAASAALRALYLDRGEVTSAIELIAKEREYTEAPNIKAKLCAEAAKLARNRLKD
                     ATRAELSAKEALEYDENQVDASTILGDLAFENDRPVEALHYYGAVIPKLEQLPKQWAI
                     STLRRFITSLARTQQSDKALAACEQLLEFAPDDLEASIIAGRILFEHGDAKRAFQVHQ
                     DILDRFGDKLVGNDKSGVLYRYGESARKAGDAIIAIKSLTESADMDPTAAEPLAALAK
                     VHESKGEWEDVIRIKSRRLDVAEGDERVQLLVDIGEICSSKLDDKTRAAKSFMAALEE
                     RPDDRKLLTRLMQLYSENKDWTKLVEVVLKLADFVEDPKQKAKYVHTAAMVTGQQIGD
                     VDKALELLDTVRQLDPDLESSITEAIKLREKKGDFEGIEKLLNLRLERATEKDDRDTV
                     LATFDALGALYKDKLGWMTEAIDAFEAAQMLDPDNTERNSALAEMYASNPTDYLDKAV
                     SSQHALLSNNPNNPAPYKLLRRLYTEAKRADGAWCLCQALAVLNLAEPDEDRFYRRMR
                     SETAAPAQDRVSPESWAKHLVHPDADPVLTSLLAVIEPAVLATRADTLENLGYDPRYA
                     IDLSLHPYPMSQTIYYAAGVLGMDPPPTFQNINDEGGISFLHAQIPSIVLGRAAFEVE
                     VPNQAAAFIVGRHLSYYRPGMYIRHLIPTGTGLKAWVFAAIKMNSPQFPISADLEGPI
                     AENLNALNVHLTGASRERLASLVSKLLQSSGAMNLKKWVAAVDLTADRAGLLVAHDLE
                     IAAEMIKASDESISPVTHKDRLRELVLYSISEPYLALRQQLGIAIDS"
     gene            6986..7891
                     /locus_tag="PK710_01305"
     CDS             6986..7891
                     /locus_tag="PK710_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HOT08368.1"
                     /translation="MANQGWAPEDLDDFGDEPPQWLRIGVAMSSSSESIGGSPTGPTP
                     SQLDALCRALAPRIGIAVTAQAFSDYRALLSAINRGDADLAWLPPLVALRSASAGRTL
                     PIVLPIRGGAAWYASALFAQPNITVSSIRDLRSVRVAWVDPQSTAGFLVIRAWMRAQQ
                     IDFNLAFSQQTFFGSHAAVVQAVLRGHADVGATYAHLDHSLKNIRSAAWGSAPVQVIA
                     MAGPIPNDVLSASIRLPVSTIRSIRDALIHQDDALRAATSALLNAEGFIAAETEHMSP
                     LMKVLKHMDDTGRRLTSIIPPPQKP"
     gene            8008..9759
                     /locus_tag="PK710_01310"
     CDS             8008..9759
                     /locus_tag="PK710_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08369.1"
                     /translation="MPDDRFSDFEYPVTPPSSLSFARSHLATGNQGLVPVRIHEAKVE
                     VPYGTPVGAVLKDLPMDRGLTVLAAIVHHRCVDLTHPICAPTDIRPITYAHREGVLVY
                     RRSVSLLLLEAARQVFPGVRVAIGQALGNGYFYRLHLNETFTKEHARLLENRMREMVA
                     RDIPLATETVSIAEAREQFETLGLKDKLRLLKTHWEPFVTLVRCGNFCDIHRYPVVPS
                     ARHLTSFQVEHYPPGLVLRFPPRGHLSAMEPFHDTPKLFQVYQETREWNRIVGVEDVG
                     QLNDLIIRGEAADMIRVSEGLHEKKIAEIADRISSADHPVRLVLIAGPSASGKTTFAK
                     RLSLQLRVNGIRPVALSMDNFYVNREDTPRGEDGEYDFEAIEAIDLALFNEVLSKLLA
                     GERVLTPKFDFPSGLRVARERWSPMQLEPGQVLMVEGIHGLNDRLTEAIPHASKFKVY
                     VSALTQLCIDDHNRIFTSDSRFLRRIVRDRMFRGYPASMTIESWPRVRRGELRNIFPF
                     QEQANVMFNSALVYEQAVLRLFAERFLMEVPQDHPSFSAAYRLLKFVQNFAPIFETSV
                     PQISLLREFIGGSGFKY"
     gene            complement(9811..10920)
                     /locus_tag="PK710_01315"
     CDS             complement(9811..10920)
                     /locus_tag="PK710_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT08370.1"
                     /translation="MIIAHLSDFHISRHGSRLTQLQDAAWRANGESGWETIRRVGDWR
                     IQTRPPKRLHIHHRLRLVDDIGVVHRVMKVGRGYRSKTESIDMLLTFLEARERTTPQA
                     LAQKFPSTRQLERLLSLDPDNINLRFCAVAHAIRRAKPDHLVVTGDVTDDAEGFELVL
                     EGFEPFFSTGRVSIVPGNHDVYPSPPLWVAKKYRKTEAAKRMLWATFSSSLGLPPSGS
                     HVRVLERGVLLVCLDSCHRARVLGSASGLVPMGDLHEMARQLDASGKGIRIACLHHHI
                     LNFPAKGLGIAPLQPGMRLRNAKEVYAQLKELGFRLVMNGHRHVGYRFHPAMAPLFLS
                     SPSSTIGCRSGARPYYWQIEVDRHGLHQVKECPIP"
     gene            complement(11002..13155)
                     /locus_tag="PK710_01320"
     CDS             complement(11002..13155)
                     /locus_tag="PK710_01320"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOT08371.1"
                     /translation="MRSSRWLVTLGIVMGFAVGCSSNTDEEQSPVPEEDTQIVPPGKE
                     DNFLSATAQEYMVEGISTVTIESNLANASEERKLARVYELIPLKQIVIGWFLNAYIVD
                     KSNDDDNAQYGGFKGLTKNGAYEELGIEALDATTYQFKFRQEFGGKLDLLSKLPTTMG
                     ADGRRYFDLTIGKISNADMARLETNAEWYRRSPWASFDPSKVDPSRLDTVTMAVWPEP
                     RSIDAWFDTERLMEDGKVTMAIHFGWDYHNDYHLVHSREVYNWLVSSQKYKSPVASYD
                     DYRRDSGPLTKTIRANGKDVQVEISLYWGKVGTDTDPDTNAGGRQLEADMKASFRDKD
                     VIVFSGHSGPFYGFALANWKKTDEGDLDDSEIPGLDMPADKYQVVIAEGCDTYSLGQS
                     FRMNPNKPDAKNIDIVTTTSFSNASTAAAVKDSIKALVGTNSAGEHEPATVLTYLRDM
                     DDNSYWFSTMYGIHGIDDNPHRHPFAAVGNLCSPCSSNSDCGGVGNSCSRLNTNEKFC
                     TFDCTDDDGCPEGYQCMPAASGSVMSSKRCAPTNLTCTAPPPEPSGPAAILNEVFASP
                     KADGGDANGDGISHPVEDEFVEIVNVTNKAVDLTGWRVADSFMTRFAFPPGTIVEPGK
                     AVVVFGGGIESAFSSFDGASVFVSSNQLGLNNSGDRVTLTDKSGEVADEMKYGSEGGG
                     GVSLVRSTDGDVSAPWILHPSRKHSAGLRTDGSPF"
     gene            13330..15309
                     /locus_tag="PK710_01325"
     CDS             13330..15309
                     /locus_tag="PK710_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08372.1"
                     /translation="MNRERTARWFVLLIALLLVVPSRALAQGACEKHHQPWLQRCQTQ
                     TGVPMSVLRCPPGRVIVMVGEGEYATAVDIAPASSQSFRKAGELGASPIGQYADWSAE
                     PEARRHAFDALTSCLEQAPTLDRVPALSTQKKARWPWLLLAAGAVFSFRLLLGRKRGP
                     GRMRLRRQPWRACVRDACVGRGPSAWLAFGLLTFVTWSLRTLWMPPRFFHQNGQGPLW
                     IEMALTGGESAYGPGFAEVFGWLTHLSPTAPDKMVFLAMNAVCAFIPLLVWVVARCSG
                     GDVRSCWVLAACTSLHPLLARLARGESYLAVQQALLFAAAAVLAWAGRKRRSPAAFLL
                     AVMSSGLLLSQAVRIHPTSWVPTMVVPFVLLIQPLPIIKRIKRTAVAFTAIAAVLLCT
                     SFSTLWDVYSGPLGQQWPAQTMLTTPRLALGNGALVAALVVIILVGRLRIHSLLVAGT
                     AGVSILLLASADLLVRDVPWIADAYRLQILPVFAAVGVASCRILISPTMRASRILNPL
                     LLLLIAGAFTAGRWKSYRTIPTDALEQSWAMQWRQTLPAGATVAYLKRAENRVFMLPL
                     FPDGPVNIEGYDSRQMALASPLQPGMFYYRSSLCSTAEGAPACAALERDVLLETIEEN
                     EFPAIASQPWAPFHQSSLRASLHRVRKAGRSPTGS"
     gene            complement(15362..17437)
                     /locus_tag="PK710_01330"
     CDS             complement(15362..17437)
                     /locus_tag="PK710_01330"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT08373.1"
                     /translation="MDPVALASRLDDEFRTSCSLLDNDNPWFGYVTLRSGDRLREYRI
                     GRFRTVEHHIVPVTHPIARALMECEPGDDFEVDREGLNQIKGVVEIQGRLETQQRALH
                     VLHYSDVEREAVVVRGHEGFELRVDTARKIDAQTGLPDILALLSREQYRVIAQHSDSP
                     IILQGRAGSGKTSVALYRVAWMANPVMEGKVAVDPSRVLIVMFNKALSLFVRDILPSL
                     GLQQAVLDTFHGWALHRVSDAYKGDIKIDSSPYPGKEQAVRMKKRLGILHAVEVFVRE
                     QRLRIAEWLRTKLAPYKGEAWAERFDVSNEPVVRALVEIRRAARQARDVASGFERQRL
                     EQVALIFDGAVERVRKYKEELMAILTDMRLLQKCLPEESSQDLQALATYQKTLQHRDA
                     SPRRPGPYIGFEDLAILLRLIQIKNGGLPSAHDLDTVERYDHLVIDEVQDFGAVELKV
                     LLDAVRSRMGVTIAGDLNQKIVPEADFIGWDALIAELGIAGARVSRLEVAHRSTGSIM
                     RVADSILGETSNHGRFGTLPTLTFTEGEERQRECAEERIRAVLRDHPLAHVCVVARHR
                     ASVQSLSQDLGARLSKDGVDVRIGHNDSFAFRPGVTVTNMRQVKGLEFDAVIVLDATD
                     ESYPDTEQGKRNIYTVVSRAKDRLDIVCPGTLSRWLEEAKERGLVDVAEMLAVVPVSF
                     SEEELTDVF"
     gene            complement(17518..18609)
                     /locus_tag="PK710_01335"
     CDS             complement(17518..18609)
                     /locus_tag="PK710_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08374.1"
                     /translation="MYSGWVLTSCPSCHGKVSEKVAVCPHCGAPLLKPVPCRSCGEPV
                     FPGATTCSACHAPWGPPPSTAPSSMPHPAPTPPPQKTDSTASAQNKPHILKRYGFVLA
                     IGSALPVLIVVAYFLVAKSDAQCARETACEQAGLCSASFHTCVAAKDADCAKSQNCIE
                     YGECSSRQGKCIVAGDADCASLCKSTDRCVARDGVCVCDPSKAACCLQVGKCGVDEKG
                     WCSTYTEQGCQQSVACRRDGLCSLSEKKDRCIASRDEHCTQSEVCKTEGRCQVRDGGC
                     IGLNCADSEDCKVKGRCVQRDGRCVAASEADCRSSVWCIKLGRCKLEAGQCRASEQGC
                     KASENCKSDSQCYLSTDGEACVTKPGSDR"
     gene            complement(18655..19692)
                     /locus_tag="PK710_01340"
     CDS             complement(18655..19692)
                     /locus_tag="PK710_01340"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOT08375.1"
                     /translation="MTLDGNDRVQEVEEWIDQSYELLEKGDVRGAQEMYSRAASRAGS
                     DAQVLTLAGCIASERGEYAEAIERFEDAMGSNPTHYVALINAAEVYLDGLGDAQGCVE
                     VCERALPLADVPEAQFELILFKAQALTALGREDEARQSLLATKGIEVGDPALLERVGG
                     MAIALEDWDTAEGALSAALRIDPDYADAHYGMGWVCLEREHLDKMREHWLRTLELDQK
                     SPLPDWHLTPGQFDEYAEKALMEIPEKARVLLQDVPIIVEDMPSALDVEDGLDPRVLG
                     VFCGASRDEQSHLDAGPEEPNVIKLYQRNLESMCGSRKELIEEIRVTLLHETAHFFGL
                     EEEEIERLGLA"
     gene            19829..20887
                     /locus_tag="PK710_01345"
     CDS             19829..20887
                     /locus_tag="PK710_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOT08376.1"
                     /translation="MQRQEISIVFHWLWSSYPSLRANHDGLINQTFIVHESDTPVAVL
                     QRLNTAIFIPQVNEDIEAITTAIHRAGLPTPRLLRTLQGALWHQDEQGSVWRCMTYEG
                     NRTVHRLSRPLDAWQAGGLVARFHAAVHGVAWEFRSVRPAVHDTQAHMNRLEQAIARH
                     RTHRLFDDVAPLAEEIRKQWETWHGTDDLPRRIIHGDLKVSNVRFVDDSAHCLIDLDT
                     MGWGTFDMEMGDAMRSWCNPTSEDSMESRFDIAMFESAMSGYADACRKLHTDQTPTRA
                     EWESVVAGVERVTLELASRFARDALEEAYFGFNPRYGGRGEHNLLRARGQFHLAQRVR
                     EQAAEAERILHNLQTRLT"
     gene            complement(20893..21858)
                     /locus_tag="PK710_01350"
     CDS             complement(20893..21858)
                     /locus_tag="PK710_01350"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOT08377.1"
                     /translation="MTDDSRKKSSEVRFHGAAVTDVGRKRKHNEDTVLSRPDLGLFLV
                     ADGMGGHSAGDVASALAANSVEHFFEATRDSPDSELTLPEYPDLVPDAVRLVLAIRHA
                     NEQVVRAAQENPDRKGMGTTIVAVVLSSDGMIHVAHVGDSRCYRIGVGDIEQITQDHS
                     FLNDVRWSQPGIAEDVMAGIPKNIITRALGTKENVEPEIRSEMTLPGDAYLLCSDGLS
                     GMISSSTMLEVVDEIDDPEIACLELIERANQAGGKDNISVVIVRIDEREDTLPNAAVG
                     VVGPDRAPLRFDAEKDKWVCSHCGHEHIEGTSFCVECGLPFLPGG"
     gene            22034..23401
                     /locus_tag="PK710_01355"
     CDS             22034..23401
                     /locus_tag="PK710_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT08378.1"
                     /translation="MRKVQPSTVTITRPRSDSMHVETATFRLTVVSGPNQGQGFELDG
                     LAPSRVLAGTSPACEIRLSDPLISRRHCAIEQTGRRLRIADLGSTNGTFVNGVEIVEA
                     WLDGGENLLVGGTTFHVQKDPTAKTALLPARTMFGSVVGASEEMRKLYPLCERLAASS
                     VPVIIEGETGTGKEVLAESLHAEGPRANGPYVVFDCTATPPDLMESAIFGHEKGAFTG
                     ATDTRAGVFEQADGGTLLLDEIGDLHINLQPKLLRVIQSMEVQRVGGARRRKVDVRVL
                     AATRRDLDREVQAGRFRDDLYYRLAVARIELPPLRRRRGDISLLAKYFWSENENALPI
                     PTELLQRLEDYEWPGNVRELRNVVLRHQALGDLARFERPLIADSPDALMAFSDPVGEI
                     IHRLLPLAEARQEVIQLFEQRYLEHILAVNDGNVTRAAAAAGVARRYFHLLRAKYAAK
                     NTPHE"
     gene            complement(23406..24500)
                     /locus_tag="PK710_01360"
     CDS             complement(23406..24500)
                     /locus_tag="PK710_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08379.1"
                     /translation="MQANRTRLIAVLSLLALAIAAWWLWPRESPPKTTSTRRPAEPVE
                     RTVDRPSNPEPVLSVAPPTMVREDASAPSAGVIASALWGGGKDQIGRDVPEEANPEGP
                     MSLAIGPNGTLTVLDQVNGRLARYDKNGRRTFDTRLDALYPQDVTVASDGTTLVLDRL
                     KDKNVTVLDPNGNVVGQIPLEGTSIDEPGGITGVFVDGDDVYVEREHGALVKVGTKDG
                     APAQEPEELPGRPSRDGSLLLSAGIIDPEAGRIFVSAIQRATRDHLFTRELRLEEAVM
                     YLVLLDSDKHGTIYVAAVIGNPELSPGDVRVQLVCLEPTQGAPVGGAWMPANTLPDET
                     FRDAVVLDEGGVVYAERTEDGIIYRVYHCS"
     gene            24665..>24913
                     /locus_tag="PK710_01365"
     CDS             24665..>24913
                     /locus_tag="PK710_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08380.1"
                     /translation="MWYKVAGSKSFWVTLAAMSAALLIIVGACSPAAPTDSSNLESVD
                     ETVASSEEGLSGSLPTGSILKTTANLNFRTGPSTGNKVI"
BASE COUNT         5513 a         7400 c         6609 g         5391 t
ORIGIN      
        1 tcgcaacgat gagcatggcg gcgcgtccca agatccgaac cgcaacgatc gcaatctccg
       61 atcctgctgg aggagcgata tcggcaagcc attgatgagc cgcatcggtc gggaccgttc
      121 cgaaatacca tccatgcagg gcggcatgag acaacaccgt atctcgatgg cgatcgactc
      181 gaatggtttt taaagcatcg accgaagcaa acgatggtgt gcaagcccaa cctgaaaaaa
      241 cgtcatttcg caccgcgaaa attcccacgc gatgcgccac ggcctccatc gcacgaagcg
      301 cggctcgaac cacctgatct ctcgtggctg cctgttgtaa ttcctcaagg acggcctcga
      361 tttgcgccac cgactcttcg agctggaaag gcgctgatac accctcgatc gtcggcagcg
      421 ccgccgacgg aggaatggcg atatgaaatg attcgacggt ggggggatcg atcctattgt
      481 cttgccaatc ggaggtttgg agacgagttg cgggaagcga gcccgggctc aatacgatgg
      541 gctctggcgg attgtatatg tctttcgtcc gcggctggtt gcccattttc ggagcacggc
      601 ttcttcgaac cagaggaatt gggatatccg aagcggaagg acgttcccga ttttttcgta
      661 acgccttgat ttgctccaag gcgttttcca ccaccaacaa gggcgaatac acgatgcgaa
      721 tgggcgcttg cagatggtgt gcaaactctt gccggatgtg atcaccaaag ggatccgggg
      781 tagcgacatc gatggttccc gtcatgggat ctcgtcgaac gggaaccgcg agcaagcctt
      841 cgcacaatcc ctcagggagt tgataaaaca catccatggc tggcatgatg tgatgcaagg
      901 ttggggcgtt ggaacgacgg cattcgcggt cgatggcctc gaggctgacg ccactgtcgc
      961 gaaacgcgcg aacgagcgaa acggaatgct tgacatgcat cgcgagcgcg gcatgagcct
     1021 gttgttcggt catgccgtgg gaagcgagca gtcggcgtgc aagctcatag ggcacggggt
     1081 tgacgatagt cttgcctgcg gcgcctgtca cggtcgagac gggaacagaa aaacctcttc
     1141 ggtatttcgt accaagtcga gaccatcgcg ggcgattcgt tctacggccc tgggatctcg
     1201 tttcagcatc tcgacttcca cgcgtaattg acggattttt tccgataact ccgcgttctc
     1261 cgcttcaact cgcgcttttt cagcttgcag agcacgtaat cgcgggagcc cttcttgaga
     1321 gagcatgaga gcgggagcac cgacggcagc gacgcttagg ataaggatgg gcaggccacg
     1381 ttcgagaaac tgcgacaggt taaccaccgg ctaaccatgc catgaaacgg cggacagagc
     1441 caaggaagcg gttcaagctc caaaacgtct gatcttgctt tttttcgcgc aagaggcgtt
     1501 caaaccgccc cgatttccga taggattcgc caccctggtt gcgcttccct acccgacgcg
     1561 cgtggcggat aaccatgcga agagcaggac aatggataag gatactctgc gcagtgaact
     1621 cgagcgtttg tttgacctcg aagagttgat ggatgtatcg cgagacgttc tcggcatatc
     1681 tcccgacgag gttggtcata ctgcctccaa agctttgttc gcaaaagcct tggtagaccg
     1741 ttgtgtcgtg accgagcgag tggaagcgct tttggattcg ttgatcacgc tccggcctac
     1801 cgttgacagg aaagccttac agaaagtctc ttccagtctc gtggccgagc acgaacttcc
     1861 ggccggttct tcgttcggtc ctttcgtggt tcgtcgaaag gtcggttcag gtccctcggg
     1921 gtcggtatat ctcgtgaaac gtgacgaccg ggaactgact ctcaaagtgt tgcatccctt
     1981 gatggggcga gatcgcggtt cttttgcgag gttcgcggcg gccacccgtt tgttctctcg
     2041 cgtttcccat gagggtttgc cgacggcgat gacgttgggc aatgacgagg ggctcgattt
     2101 cgttgcttac gactatatcg acggccaaag tcttgcgtct cggacggcga gaagcgggcc
     2161 gatgcacatc aacgaagcgc gttctttgct tcagaagttg ctcgacgtgc ttgtggcgct
     2221 tcacgaccaa cggttggtgc atgggaacat caagctttcc aacattctgg tagcgcgagg
     2281 ctccgaaccg ggtaagcaga aactcgtatt gctcgatccc gcgttcgatc gtttacggct
     2341 gcttcgctcc tcgtcgtcga gtgatgatgt gtgtgtcgta ttgtctcatg cccgatcggt
     2401 atcgcccgag cagctacggg gagttggcgc ggacgaaagg tccgacgttt attctttcgg
     2461 cgcggtcatg tacgaaatcc tgacgggtaa gcccttattc tctgctggtt ccgccctcga
     2521 atatgcgatt gccacctgga cgcaggatcc agaaccgccg atccaggcag ccccgcgcgg
     2581 ttgggtagcc aaggaggttt ctgatttcat tatgagcctg ttggctcgtg aaccaagtct
     2641 tcgccccaaa gacgcgcggg cggtactcga gcgtttcgag atgttgggca gagtcgcgac
     2701 gcaggatgat tctaccaaca agatatcgga cgaagagctt agcgaacgca ttgatgccct
     2761 ggtcatttct cctgatgacg aagcggcgat tgcagccctt gacgaggcag cggaacgtgg
     2821 cggggatgtc gccaaaatcg cacaagcctt ggtcatggca gcggatcaaa tcgagacaac
     2881 cgatagtgac ggagttgagc ttcgaaagag tctgctgttc cgctcggcgc gcttgtacga
     2941 atccaagctc aaggatttgc cggaggcgga aaagctctat gtatggatca gcgaattgga
     3001 tcccgccgac gaaatcgccg acgcagcgct cgaggaggtt cgtcgccaac ttggcaagac
     3061 cgaggaagtg gtggagatgc tattagctcg gatcgagcgt gcggccgact ccgaggagaa
     3121 ggcgacggcc atggcggaga tcggccgatt gtacgcttct gaactcgaag acgcttcgca
     3181 ggcggtcgtg gcctatgccc aagctttcgt cgaatcgccc aaacacgttg aattcgcgga
     3241 tgcgatcgaa agaatcgcgg gctcggatgt caacgtactc ggcgaggcga tggcgatcct
     3301 agcggagagc acccaaaccg atatccctat agaaaacaag aacttactat tccttcgtct
     3361 cggccattgg tatggaaagc ggctcggtcg gtatgacgcg gcccttcctt gttaccaggc
     3421 cgtcatcgcc accgatccag tcaacgatgg cgcgctcgag ggaatggcgg ccttgttcaa
     3481 ggccaatcag caatacatgg aactgggcca agtgttgatg cgtcgtgcgg aagccgcaag
     3541 cacccctgcc cgctcgcgcg agtttcgcct cgatgcggcg gagttgctcg aaacccgtct
     3601 tggagaacca gcgaaagcca aggaattgta tgagcaagtc cttgccgaag atccttctca
     3661 cgacagagcc accgaagctt tgcttggcat ttacgaaaag gaagacaacg ccgccagctt
     3721 ggtaaaaatc ctcacgaaca aagcggagcg tctcagcgga tcacaagctg ccgacatttg
     3781 ggttcgtgct ggtgatgtct acgaaaccaa actcaaggac atgaccaatg ccattcgttg
     3841 tttcgaaagt gccgtacaac aggacgaaac gaaccaacgg gcgctcgacg cgctggaccg
     3901 actccacacc caaagccgtc atttcaagga attgcttgcc accatcgagc gaaaaatcaa
     3961 catcgcaacc acgccgcgac agaaaatcgt tcatctcgag cgaatcagca aactgcatgc
     4021 agaagagttc ctcgaccacg tggccgcggc acaatccttg gagaagattc tagaaatcga
     4081 tcccgcccac agtgccactc tcgaagcctt ggcgcatcac tatcgcgcgc ttgaccgctg
     4141 gagcgatgta gcgaacgtgt atgagaggga cttgaatcta cagacggaca aagccaagcg
     4201 actcgatgtg ttgctgatgc ttgcgcgtgt ggtggccgaa cgtctgagcg atcctgcgcg
     4261 agcgatgaag gtatacgaac aagcgttgga gatcgagcct ggtcatgcgg gggcgctcga
     4321 agcccttgcg gcgcttcgtc aacagtctgg cgattcccaa gcagcgttgc aagcgatcga
     4381 agcgcttgcg gaaaaggctt cgagtcccaa tgaaaaagca gagcaataca ttcgcgcggc
     4441 caagcttttg caggagcgcg acgacgtcga aggggcgatg gagcggtatc ggcttgcctt
     4501 ggacgccaac cctcgagcgt tcgccgcgtc tgcggcactg cgtgcgttgt atctcgatcg
     4561 tggcgaggtc acatccgcca tcgaactcat tgccaaagag cgtgagtata cggaagctcc
     4621 caacataaaa gccaagcttt gtgcggaagc agccaagctg gcgcgcaatc gtctcaagga
     4681 cgcgacccgc gccgagcttt cggccaaaga ggcccttgaa tacgatgaaa accaagtgga
     4741 tgcatcgacc atcctcggag acctcgcctt cgaaaacgac cgtccggtcg aggcgcttca
     4801 ttactatggc gcagtgattc cgaaactcga gcagcttccc aaacaatggg cgatctcgac
     4861 actgcgtcga ttcatcacca gtcttgctcg aacgcaacaa tcggacaaag ctctcgcagc
     4921 ctgtgagcaa ttgttggaat tcgcgcccga cgatttggaa gcctccatca ttgcggggcg
     4981 aattctgttc gagcacggtg atgccaaacg tgccttccaa gtccaccagg acatccttga
     5041 ccgattcgga gacaaactcg ttggcaatga taagtccggg gtgctgtatc gttacggcga
     5101 gtccgctcga aaagctggcg atgcgatcat tgccatcaaa tccctaacgg aatcggccga
     5161 catggatccg acggcagcgg agccgttggc cgctcttgcc aaagtccacg aatccaaagg
     5221 ggaatgggaa gatgtcatcc gaatcaagag ccgtcgtctc gacgtggcgg aaggagatga
     5281 acgagttcaa ctgctcgtgg acatcggtga gatttgttcg agcaagctcg atgataaaac
     5341 gcgagctgcg aaaagcttca tggccgcgct ggaggagcgt cccgatgacc gcaaactgct
     5401 cacgcggttg atgcagctat attccgaaaa caaagactgg accaagcttg tcgaggtcgt
     5461 gctcaagctc gccgacttcg tggaagatcc aaagcaaaaa gcaaagtacg tccatacggc
     5521 cgccatggta acgggccagc agatcggaga tgtggacaag gcgctcgaac tgctggacac
     5581 cgtccggcaa ttggacccgg accttgaatc ctccatcacc gaagctataa aactacgcga
     5641 aaagaaaggg gatttcgagg gaatcgaaaa acttctcaac cttcggctcg agcgtgcgac
     5701 ggaaaaggat gatcgggata cggtattggc gaccttcgac gctcttggag cgttgtacaa
     5761 ggacaagctt ggctggatga ccgaggccat cgatgcgttc gaagccgctc agatgctcga
     5821 tcctgacaat accgagagaa actcggcgct cgctgagatg tatgcctcca atcccaccga
     5881 ttaccttgac aaggctgtgt catcacaaca tgccctgctt tccaacaatc ccaacaaccc
     5941 cgcgccctat aagcttcttc gtcgtttgta tacggaggcc aaacgagccg acggcgcgtg
     6001 gtgtttgtgc caggcccttg cggttttgaa tcttgccgaa cccgatgagg ataggtttta
     6061 tcgccgcatg cggtccgaaa cggccgcgcc cgctcaagat cgcgtaagtc ccgaaagctg
     6121 ggccaagcat ctcgtacacc ctgatgcgga ccctgttttg acgtcgctgc tagcggtcat
     6181 cgaacccgcg gtgctggcta cccgcgcgga tacgctggag aacttgggct atgacccgcg
     6241 ctacgcgatt gatctatcgc ttcatcctta cccgatgtcg cagacgatat attacgcggc
     6301 cggtgttctg ggcatggacc cgccgccaac tttccaaaac atcaatgatg agggaggcat
     6361 ctcgttctta catgcccaga tcccatcgat tgtgttggga agagctgctt tcgaggtcga
     6421 agtacccaat caggctgccg cattcatcgt cggtcgccac ttatcctatt accggcccgg
     6481 gatgtacatt cgtcatctca ttccgacggg aacgggattg aaggcctggg tgtttgccgc
     6541 catcaagatg aattcgcccc aattccccat ttcggcggat ttggaagggc cgattgcgga
     6601 aaacctcaac gcgttgaacg tccatcttac aggcgccagt cgtgaacggt tggcgagtct
     6661 cgtatccaag cttcttcaat ccagtggagc catgaacctc aaaaaatggg tcgctgcggt
     6721 agatctcacg gcggaccgcg ctggactgct ggtggcccac gacctggaaa tcgccgcgga
     6781 gatgatcaaa gcctctgacg agtccatttc ccccgtgacc cataaagacc gtctgcgcga
     6841 acttgttcta tactccatta gcgagccgta tttggcgctt cggcaacaat tgggcatcgc
     6901 aatcgatagc taacggcccc tgtacggcaa ccattgtttt gaccacagtt gaccgcaaag
     6961 cgcgtccacg caataggtaa gcttcatggc taaccagggt tgggcacctg aagatctgga
     7021 cgatttcggc gatgagcctc cgcaatggct tcgcattggt gtggcgatgt cgtcctcgtc
     7081 cgaatcgatt ggcggttcac ccacggggcc gacaccatcg cagctcgacg cgttatgtcg
     7141 cgcgttggca ccgcgcattg gcattgcggt tacggctcag gcattttccg actatcgagc
     7201 gctgttatcc gccatcaatc gaggggatgc ggacttggct tggctgccac cgttggtggc
     7261 tttgcgctcg gccagcgcgg gtcgtacctt gccgattgtc ctccccattc ggggaggagc
     7321 cgcctggtat gcctcggctc tatttgcgca acccaatatc accgtatcgt cgattcgcga
     7381 tcttcgttcc gttcgcgtcg cttgggtcga tccccaaagc acggctggat ttcttgtgat
     7441 tcgagcttgg atgcgcgcac aacaaatcga cttcaatctc gccttttcgc agcaaacctt
     7501 ctttggctcc catgcggctg tcgtacaagc tgtccttcgt ggtcacgccg acgttggggc
     7561 cacgtacgcc catctcgatc acagtctgaa aaacattcgt tccgcagcct gggggtcagc
     7621 accggttcag gtcattgcga tggcgggacc gatcccaaac gatgttcttt cggcaagtat
     7681 tcgacttcct gtttccacga ttcgttcgat acgagatgcg ctcatccatc aggacgacgc
     7741 tctgcgagcg gcaacttccg ctttgctcaa cgcggaaggc tttatcgccg cggaaacgga
     7801 gcatatgtca ccgctgatga aagtcctcaa gcacatggat gacactgggc gccgcttgac
     7861 ctccatcatc cctcctccgc aaaaaccttg atccaaaatc cgcttgctgc gggatcgtcg
     7921 tcacgaacgt tgcggcactt caacgcatcg agtaataagg agttttctca cttatgtgtc
     7981 cattctctaa ctcgtggtga ggtttccgtg cccgacgatc gcttttccga cttcgagtac
     8041 cccgtgactc ctccttcctc cctatcgttt gcgcgaagcc atctcgcgac aggaaaccag
     8101 ggccttgtgc ccgttcgaat ccacgaagcc aaggtggagg ttccgtatgg gacacccgtt
     8161 ggagccgttc tcaaagacct tcccatggat cgagggctca ccgtgctcgc tgccattgtg
     8221 catcatcgat gtgtggatct tacacatccg atttgcgcac cgacggacat acgccccatc
     8281 acgtatgcac atcgcgaagg cgttctggtt tatcgtcgat cggtgtcctt gctattgctg
     8341 gaagcggctc gacaggtttt tcctggagtt cgcgtagcca tcggccaagc gctgggaaac
     8401 ggctacttct atcgtctcca tctcaacgaa acgtttacca aagaacatgc acgccttctt
     8461 gaaaacagaa tgcgcgaaat ggttgctcgg gacatccccc tcgcgaccga aaccgtaagc
     8521 atcgcggaag cgcgcgaaca attcgagacc ctcggcttga aggacaagct tcgcctgttg
     8581 aaaacgcatt gggaaccatt tgtcacactc gtacgatgtg gaaatttttg tgatattcac
     8641 cggtatcctg tggtcccgag cgccaggcac ctcacctcat tccaagtcga gcattatccg
     8701 cctggacttg tccttcgctt tccgcctcga ggccatctat cggcgatgga accattccac
     8761 gacaccccca aactctttca agtctaccaa gaaacaaggg aatggaaccg cattgttggt
     8821 gtcgaagatg tggggcaact gaatgacttg atcattcggg gggaagccgc cgatatgatt
     8881 cgtgtttccg aggggttaca cgagaaaaaa atcgccgaga tcgcggatcg aatcagcagc
     8941 gcggaccacc cagtccgact cgttctcatc gcgggcccgt ctgcttcagg aaagaccacc
     9001 ttcgccaaac ggttgagtct tcaactgcgg gtcaatggca tccgtcccgt cgccctctcg
     9061 atggacaact tctatgtgaa ccgcgaggac acaccccgag gagaggatgg agaatatgac
     9121 ttcgaagcca tcgaagccat cgatcttgcg ttgttcaacg aagtcctctc caagttgctc
     9181 gcgggcgaac gggtgctgac accgaaattc gactttccga gcggcctgcg cgtggcccgt
     9241 gaacgatggt ctcccatgca actcgaaccc ggccaggttc tcatggtcga gggaattcat
     9301 gggttgaacg atcgactcac ggaagcgatt ccccacgcca gcaaattcaa ggtctacgtg
     9361 agtgcgttga cgcagttgtg tatcgatgat cacaaccgga tattcacctc ggacagtcgt
     9421 tttttgcgca gaatcgtacg cgatcgcatg ttccgcggat atccagcgtc gatgacgatc
     9481 gagagctggc cccgggtgcg acgtggggag cttcggaaca tctttccttt ccaggagcaa
     9541 gcgaatgtga tgttcaactc ggctcttgtc tatgagcaag cggtgcttcg attgtttgcg
     9601 gaacgattcc tcatggaagt cccacaagat catccgagct tcagcgctgc ctaccgactt
     9661 ctcaaattcg ttcagaactt cgcccctatc ttcgaaactt ccgttccgca aatctcccta
     9721 ttgcgagaat tcattggagg aagcgggttc aagtactgaa tcctgccttt ggctttcgaa
     9781 gccctcaatc ccgacatcta cttatcgaag ctacggaatt ggacattcct tcacctggtg
     9841 aagcccgtgg cgatccactt caatctgcca ataatacggt cgcgcgccag agcgacagcc
     9901 gatcgtcgag gaaggggaac tgagaaacag aggagccatg gcaggatgaa agcggtatcc
     9961 gacgtgccga tggccgttca tcaccaaccg aaagccaagt tccttgagtt gcgcgtatac
    10021 ctcttttgca tttcgaaggc gcatacccgg ttggagcggg gcgataccaa gccctttagc
    10081 aggaaaattg agtatgtggt ggtgaagaca agcgatccga attccttttc ccgatgcatc
    10141 gagttgccgc gccatctcat gaagatcgcc catgggcacg agtcccgaag cgctaccgag
    10201 gactcgagcg cgatggcaac tatccaaaca gacgagcagc acgccgcgtt cgagcacgcg
    10261 aacatggctc cccgaaggcg gaagtccaag gctcgaggaa aacgtagccc aaagcatccg
    10321 cttggccgct tccgtttttc gatatttttt cgccacccac aggggcggcg acggatagac
    10381 atcgtgattg cccggtacga tggaaacgcg gccagttgag aaaaacggtt cgaacccctc
    10441 gagaaccaac tcaaagccct ccgcgtcatc cgtaacgtct cccgtcacca ccaaatggtc
    10501 gggcttagct cttcgaatcg catgagcaac cgcacaaaac cgcaagttga tattgtccgg
    10561 gtcgagggaa agaagcctct cgagctgtcg cgtcgaagga aatttttggg ctaacgcctg
    10621 gggcgtcgta cgctcccgcg cttccaaaaa cgtgagaagc atatcgatgc tttccgtttt
    10681 cgaccggtat ccccggccca ctttcatcac gcgatgaacc acaccaatat cgtcaaccaa
    10741 ccgcaggcga tgatggatat gaagacgctt ggggggacgc gtttgaatac gccaatcacc
    10801 caccctgcgt atcgtctccc agcccgattc gccattcgcc cgccaagccg cgtcttgcag
    10861 ttgcgtcaat cgagaaccgt ggcgactgat gtgaaaatcg gaaagatgag caatgatcat
    10921 gatggatcga gatgcacaga atcgcagctc gacatgacaa ccgcaaggat gggccgaaaa
    10981 gctcgggaag ccaactgctt atcagaacgg agaaccgtcg gtgcgtagac cagcggagtg
    11041 tttgcgcgag ggatgaagaa tccaaggggc actcacgtcg ccgtcggtcg accggacgag
    11101 agaaacgcca ccaccgccct cgcttccgta tttcatctca tcggcaactt cgcccgattt
    11161 atccgtcaaa gtcacgcggt cgccggagtt attgaggccg agctgattgg aggagacgaa
    11221 cacggatgca ccatcgaagc tagaaaacgc gctttcgatc cctcctccga atacgacgac
    11281 ggcttttccc ggttcgacga tggttcctgg tgggaaagca aatcgggtca taaacgaatc
    11341 ggccaccctc cagcctgtca aatcgactgc tttgttcgtt acgttgacga tctcgacgaa
    11401 ttcatcttcc acaggatgcg agataccgtc accgttggca tctccgccat cggccttggg
    11461 agaagcgaaa acctcgttga gaatggcagc aggccccgaa ggctctgggg gaggtgccgt
    11521 gcaagtcaag ttggtcggcg cgcagcgctt cgacgacatc accgacccag aagctgctgg
    11581 catacactga tatccttccg gacacccatc atcgtcggtg caatcgaagg tgcagaattt
    11641 ctcgttggtg ttcaaacgag agcacgaatt gcccacacca ccgcaatcgg aattggagga
    11701 acacgggctg cacaggttcc cgacagcggc aaacggatga cgatgcgggt tgtcgtcgat
    11761 tccgtgaatc ccgtacatcg tcgaaaacca ataggaattg tcatccatat cgcgaaggta
    11821 cgtcaacacg gtagcgggtt catgttctcc ggccgaattg gttcccacca acgctttgat
    11881 gctgtccttc accgccgcgg cggtcgaagc attggaaaaa ctcgtcgtcg tcacgatatc
    11941 gatattcttg gcgtcaggct tgttcggatt cattcggaat gattgcccaa gggaataggt
    12001 gtcgcatcct tccgcgatca cgacttgata cttgtccgca ggcatatcca agccggggat
    12061 ctccgagtca tccaaatcgc cttcgtcggt ctttttccaa ttcgccagag caaaaccata
    12121 aaacggaccg gagtgaccgg aaaacacgat gacgtctttg tcccggaacg aggctttcat
    12181 atctgcctcg agttgccttc cgcccgcatt cgtatccgga tccgtatcgg tacccacttt
    12241 gccccagtac agggagatct ccacctgcac gtctttacca ttcgcccgaa tcgttttggt
    12301 caaaggccca gaatcccgac gataatcatc gtaggaagcg accggtgact tgtacttctg
    12361 cgaactgaca agccagttgt acacctcacg ggaatgcacc aggtggtagt cgttgtggta
    12421 atcccacccg aaatggatag ccatggtgac cttgccgtct tccatcagtc gttcggtatc
    12481 gaaccatgca tcgatggagc gcggctccgg ccaaacagcc atcgtcaccg tatcgagccg
    12541 actcggatcc accttggaag ggtcaaagct tgcccagggg gaccttcgat accactcggc
    12601 attcgtttcg agcctggcca tgtcggcatt ggaaatcttg ccaatggtca aatcgaaata
    12661 tctccgacca tccgccccca tcgtggtggg caacttgctg agcaagtcga gctttcctcc
    12721 aaactcctga cgaaacttga attggtacgt cgtagcatcc aacgcctcaa ttccaagctc
    12781 ctcatacgca ccatttttgg tcagtccctt gaacccaccg tattgcgcgt tgtcatcgtc
    12841 attgctcttg tccacaatgt acgcgttgag gaaccatcca atcacaatct gcttgagcgg
    12901 aatgagttcg tagacgcgcg caagtttcct ttcctccgat gcattcgcca aattgctttc
    12961 gatcgtgacc gtgctgatcc cctcgaccat gtactcttga gcggtcgccg ataagaaatt
    13021 gtcctccttg ccaggcggca cgatctgtgt gtcttcctcg ggcacagggc tctgctcttc
    13081 atcggtgttg gaggagcatc caacggcaaa tcccatcacg atccctagag ttacaagcca
    13141 gcggctggaa cgcatcacta cctccactta agttgtccca catccgacta ccacagatcg
    13201 gaagcgtgtc aatgcaaagg aaacggattt ttttgatgcc acaatttctc gggatatgca
    13261 tcaccgaaaa gcccaaaata cctacgaacg attcccctgc acacccccca agcgtcgtaa
    13321 tcttcatgaa tgaaccgcga acgaacggcc cgttggttcg tcctccttat cgctttgctc
    13381 ctcgttgttc ccagccgagc ccttgcccaa ggcgcctgtg aaaaacacca tcaaccctgg
    13441 ctccaacggt gccaaacgca aaccggggtg cccatgtccg tgcttcgatg ccccccaggt
    13501 cgcgtcatcg tgatggtggg cgagggcgaa tacgcgacgg ccgtggatat tgccccagcc
    13561 tcgagccaat cgtttcgaaa agcaggcgag cttggcgcct cccccatcgg gcagtatgcc
    13621 gattggtctg cggaaccgga agcgcgaaga catgctttcg atgcgctgac atcctgtttg
    13681 gaacaagcac ccaccctcga tcgggtccct gccctctcca cgcagaaaaa agcgcgatgg
    13741 ccgtggctgc tgctggccgc aggggcggtt ttttcctttc gtttgctgtt ggggcgaaaa
    13801 cgcggacctg ggcggatgcg gctccgccgt caaccatggc gcgcttgcgt tcgcgatgcc
    13861 tgtgtcggcc gcgggccgag tgcatggctt gcctttggtc ttctgacatt cgtcacctgg
    13921 agcctgcgca cgttgtggat gcctccacgt tttttccacc aaaatggtca aggtccgctt
    13981 tggatcgaaa tggccctgac tggaggcgaa tctgcctatg gccctggatt tgctgaagta
    14041 tttggctggc tgacccacct gtccccaacc gctcccgaca aaatggtgtt cctcgccatg
    14101 aatgccgtct gtgccttcat ccccctgttg gtgtgggttg tcgcccgttg ttccggcggg
    14161 gacgtgcgct catgctgggt gctggccgcc tgcacgagcc tgcatccgct gcttgctcgt
    14221 ctcgctcggg gagagtcgta ccttgccgtg cagcaagccc tgttgttcgc ggccgcggcg
    14281 gtcttggcct gggccggccg caaacgacgt tcccccgctg cattcctgtt ggcggtgatg
    14341 agcagcggcc ttcttttatc tcaagctgtc cgtattcatc ccacgagttg ggtgcccaca
    14401 atggtcgttc ctttcgttct gctcattcaa cctttgccca tcattaaacg aatcaaacga
    14461 acggccgtgg ccttcaccgc catcgctgcc gtgctccttt gcacatcgtt ttccacgttg
    14521 tgggacgtat actcgggccc ccttggacaa caatggccgg cgcaaaccat gctaacgaca
    14581 ccacggcttg ccctcgggaa tggagcgctc gttgcagcgt tggtcgtcat catccttgtc
    14641 ggacgcttgc ggattcattc cttgttggtc gcgggcaccg ctggcgtgtc gatcctgctg
    14701 ctagcctccg cggatctact tgtgcgtgac gtcccctgga ttgcggatgc ttatcgactt
    14761 cagatactcc cggtattcgc cgctgttggc gtggcctcct gtcgtatcct catttctcct
    14821 accatgcgcg ccagccgtat tctcaaccca ctattgttgc tcctgatcgc cggggctttc
    14881 accgcgggtc gatggaaatc atacaggaca atccctacgg acgcgctgga gcagtcctgg
    14941 gcgatgcaat ggcggcaaac actgcccgcg ggagcgacgg tggcatatct caaacgcgct
    15001 gagaaccgag tcttcatgct tccgctattt cccgatggac cggtgaatat cgagggatac
    15061 gactcgaggc aaatggcctt ggcttcgcct cttcagcccg gaatgtttta ctatcggagt
    15121 tccttatgct ccaccgcgga aggggccccc gcttgcgccg cgttagagcg cgatgtactt
    15181 ctcgaaacga tcgaggaaaa cgaatttcct gccatcgcaa gccagccttg ggctcccttc
    15241 caccaaagct cccttcgagc cagccttcat cgtgtccgaa aggcaggtcg gtcccccacg
    15301 ggttcataac taacgaataa tattacattt gttccggtat cgagttccgt ggcctgggat
    15361 ttcaaaacac atccgtcagt tcttcctccg aaaaagacac cggcaccacc gcaagcatct
    15421 ccgccacatc gacaaggcca cgctccttgg cctcttcgag ccatcggctc aacgttcccg
    15481 gacacacgat atcgagcctg tccttcgctc gcgatacgac cgtatagata tttcgtttcc
    15541 cttgctccgt atccggatag ctctcatccg tagcatcgag cacaatcacc gcatcaaact
    15601 ccaatccttt gacttgacgc atgttggtga ccgtcacgcc cggtcgaaac gcaaacgagt
    15661 cgttgtgccc gatgcgcaca tccactccat ccttggacag ccgggcgccg aggtcttgcg
    15721 ataggctttg cacgctggct cggtgtcgcg cgacgacaca aacgtgtgcc aacggatgat
    15781 ctcggagcac tgcgcggatt ctctcttccg cacattcgcg ttgcctctcc tcgccttcgg
    15841 tgaacgtaag ggttggcaag gtgccaaaac gtccgtgatt gctggtttct ccaaggatac
    15901 tgtcagccac gcgcatgata ctgcccgtcg aacgatgggc cacttcgagc ctggacaccc
    15961 ttgctcccgc gatgccaagc tcggcaatca acgcgtccca cccgatgaag tctgcttccg
    16021 gcacgatctt ttgattgagg tcacccgcaa tcgtcacccc cattctcgac cgcacggcgt
    16081 ccaatagcac cttgagttcg acggctccga agtcctgaac ctcatcgatg acgagatggt
    16141 cgtatcgttc cacggtatcc aagtcatgag cgctcggaag cccaccgttt ttgatttgaa
    16201 tgaggcgaag caggatcgcg agatcttcga aaccaatata gggcccgggt cgtcgaggcg
    16261 aagcgtcgcg atgttgaagt gttttttgat aggtagccag cgcttgaagg tcttgcgatg
    16321 actcctcggg caagcatttt tgcaacagtc gcatatccgt gaggatggcc atcaactcct
    16381 ctttgtattt gcgcacccgt tccacggcgc catcaaagat gagtgccact tgttcgagac
    16441 gttgccgttc aaaccccgac gccacatcgc gcgcctgcct tgccgcgcgc cgaatttcca
    16501 cgagcgcacg aacgaccggc tcgttggaca cgtcgaaacg ttccgcccag gcttctcctt
    16561 tgtacggagc aagcttggta cgaagccatt cagcgattcg aaggcgctgc tcacgaacga
    16621 atacctcgac cgcatgcaga ataccgagac gtttcttcat ccgcaccgct tgctctttgc
    16681 ctggataggg cgaggaatcg attttgatat cccctttgta ggcgtcagaa acgcgatgaa
    16741 gcgcccaccc gtgaaacgtg tcgagcactg cctgctgaag ccccaacgac ggcaagatat
    16801 cgcgtacgaa caagctcaag gccttgttga acatcacaat gagaacccgc gaaggatcca
    16861 ccgcaacttt tccctccatc accggattgg ccatccacgc tactcgatac aacgccacgg
    16921 aggtttttcc ggaacccgcg cgcccctgaa ggatgatcgg tgaatcggaa tgctgagcga
    16981 tcacacgata ctgctctcgc gacaacaacg cgagaatgtc cggtaatccc gtttgcgcat
    17041 cgatctttcg agctgtatcg acgcgaagct caaacccttc atggccacga accacaaccg
    17101 cttcgcgctc cacatccgag taatgcaaca catgaagcgc tcgttgttgc gtctccaaac
    17161 gcccttgaat ctcgaccacc cctttgattt ggttcagccc ttcccgatcc acctcgaaat
    17221 catcccctgg ctcgcattcc atcaaggccc gagcaatagg atgcgtcacc ggaacgatat
    17281 gatgctcgac cgtccgaaac cttccaatcc gatactcccg tagtcgatcg ccacttcgaa
    17341 gcgtcacata accaaaccac ggattgtcgt tgtccagcag ggaacaagac gttcgaaact
    17401 cgtcatcgag ccgactcgcg agcgcaacgg gatccattgt cgacgactcc taggtgtgaa
    17461 gttgttttcg attccaccca gaatacacca attgcgtgcc gttcggcctc tcgagactca
    17521 tcgatccgaa ccaggcttgg tgacacaagc ctcaccatcg gtggaaagat aacattgact
    17581 gtcggacttg cagttctccg acgctttgca cccctgttcc gacgctcgac actggccggc
    17641 ctcgagcttg caacgaccaa gcttgataca ccacacggag cttcgacaat ccgcttccga
    17701 agccgctaca caccgtccat cgcgctgtac gcaacgcccc ttgaccttgc aatcctcaga
    17761 atccgcgcaa ttcagcccaa tacaaccccc atcgcgaacc tgacaacggc cctcggtctt
    17821 gcatacttcg gactgcgtgc aatgctcgtc gcgacttgca atgcaccgat ctttcttttc
    17881 cgacaagctg caaagtccat cgcgacgaca cgccaccgat tgctgacagc cttgctcggt
    17941 atacgtcgaa caccagcctt tctcatcaac accgcatttg ccaacttgca aacagcaagc
    18001 ggctttcgac ggatcgcaca cacatactcc gtcccgtgcc acacaccggt ccgtgctttt
    18061 gcacaacgat gcgcaatccg catcgccagc aacgatgcac ttgccctggc gcgatgagca
    18121 ttcaccgtat tcgatgcagt tctgcgattt ggcgcaatcc gcatcttttg ccgccacgca
    18181 tgtatgaaac gatgcggagc acagccccgc ctgctcgcaa gctgtttcgc gcgcacattg
    18241 cgcatcggat tttgcgacga gaaaataggc gacgacgatc aaaaccggga gggcagatcc
    18301 aatggccaga acaaaaccat agcgtttcag gatatgcggt ttgttttgcg ccgaagccgt
    18361 tgagtcggtc ttttggggag gtggagtagg cgcaggatgt ggcatcgaac ttggagccgt
    18421 ggaaggcggg ggtccccaag gggcatgaca cgccgagcag gtggtagcgc cgggaaatac
    18481 cggctcaccg caggatcgac acggaacggg tttgagcaaa ggtgccccgc aatgagggca
    18541 aaccgctact ttttcagata cttttccgtg acaggaagga caggaagtaa gcacccatcc
    18601 actgtacacg atccatggtc atgggttcga accttccgcg atgggtggca agcttcaagc
    18661 cagacccaat ctttcgattt cctcctcctc gagaccaaaa aagtgcgctg tttcgtgaag
    18721 caacgtgact cgaatctcct cgatgagttc ttttcgagaa ccacacatcg actccaaatt
    18781 tctctggtac agcttgatca cattcggttc ctcgggcccc gcgtcgagat ggctttgttc
    18841 atcccgtgaa gcgccgcaaa atacgccaag aacacgtgga tcgagaccgt cttccacgtc
    18901 caaagccgac ggcatatctt ccacgatgat gggaacgtcc tgaagcaaca cccgtgcttt
    18961 ttcaggaatc tccatcaagg ctttttccgc gtattcatcg aattgcccgg gtgtcaaatg
    19021 ccaatccgga agtggacttt tctggtcgag ttccagggtg cgcaaccaat gctcgcgcat
    19081 cttatccaga tgctcgcgtt ccaaacacac ccaccccatc ccataatgcg catccgcata
    19141 atccggatcg atgcgaagag ccgcggataa cgctccttcc gcggtatccc agtcttcgag
    19201 cgctatggcc attccgccaa cccgctccaa caacgctgga tcaccaactt cgatcccctt
    19261 ggtcgccagc aacgactgac gcgcttcgtc ttcacgacca agcgccgtca aagcttgtgc
    19321 tttgaaaagg attaactcga actgcgcttc cggcacgtcc gccaacggca aggcccgttc
    19381 acacacctct acacagcctt gcgcatcgcc aagcccatcg agatacacct ccgccgcgtt
    19441 tatcaacgcc acataatgcg tcggattgga acccatcgca tcctcgaacc gttcaatcgc
    19501 ttccgcatac tcccctcgct cgctcgcaat acagcctgcc agcgtaagca cctgcgcgtc
    19561 actgcccgcc cgactcgctg cacgagaata catctcctgg gctccccgca catccccttt
    19621 ctccagcaat tcataggatt gatcgatcca ttcctcgacc tcctgcaccc tatcgttacc
    19681 atccaacgtc atgcttacct cgcctttctc cgacactgct acgcgctggt cggaacaagg
    19741 gcaaggcaaa tacgaacgaa ccggatgagc tgcgaggcca cccgtaatag tgctggcgaa
    19801 tgtcgaaaac aacgtgtagg gacgtcccat gcagcgccaa gaaatctcca ttgttttcca
    19861 ttggttatgg tcttcttatc cttcccttcg tgccaatcac gacggtctca tcaaccagac
    19921 attcatcgtt catgagagcg atacccccgt ggccgtgctt caacggctca ataccgccat
    19981 cttcattccc caggtgaatg aagatattga ggccatcacg acggctatcc accgtgcggg
    20041 tctgcccacg ccccgtctgc ttcgaaccct tcaaggtgcg ctgtggcatc aagacgagca
    20101 agggtcggta tggcgctgca tgacctatga aggaaatcgt acggttcacc ggctgagtcg
    20161 gccgctggat gcatggcaag cgggaggact ggtggctcga tttcatgcgg ccgtccacgg
    20221 cgtggcgtgg gagtttcgaa gcgtacgccc cgccgtgcat gacacccaag cccatatgaa
    20281 tcgactcgaa caggccatcg cacgccaccg aacgcaccga ttgttcgatg atgtggctcc
    20341 tttggcggaa gagattcgaa agcaatggga gacttggcat ggaacggatg atcttcctcg
    20401 acgcatcatc cacggtgatt tgaaagtctc caatgttcgg ttcgtggatg atagcgctca
    20461 ctgcctgatt gatttggaca ccatgggctg gggaaccttc gacatggaaa tgggcgatgc
    20521 catgcgttct tggtgcaacc ccacgtccga ggactccatg gaaagccgat tcgatattgc
    20581 gatgttcgaa tccgccatga gcggctacgc ggacgcttgc agaaagctac ataccgacca
    20641 gacgccgacc agggcggaat gggaatccgt ggttgctggg gtggaacgtg taaccctcga
    20701 gctggcatcg cggtttgcac gggatgcgtt ggaagaagcc tattttggct tcaacccacg
    20761 atatggcgga agaggtgagc acaacctgtt gcgcgcgcgg gggcaatttc atctggcgca
    20821 gagggttcgc gagcaagcag ccgaggcgga gcgcatcctg cataacctcc aaacacgcct
    20881 gacctgacgc tgtcatcctc ccggcaaaaa cggcagccca cactccacac aaaacgaagt
    20941 cccctcgatg tgctcgtgtc cacaatgcga acacacccat ttgtcctttt ccgcatcgaa
    21001 tcgcagcggc gcgcgatcgg ggcccaccac ccccacagcc gcattgggca gggtatcttc
    21061 tcgttcgtcg attcgcacga tcacgacgga aatattgtct tttcccccag cttgattggc
    21121 tcgttcaatc agttccaaac acgctatctc agggtcgtcg atctcatcga cgacttcgag
    21181 catcgtcgaa gacgaaatca tgccgctcag gccgtcggaa cagagaagat aagcatctcc
    21241 tggcaacgtc atctcagaac gtatttccgg ctcgacgttc tctttggtcc ccagcgcacg
    21301 agtaatgatg tttttgggaa tgcccgccat cacatcttcc gcgattcctg gttgagacca
    21361 tcgaacgtca ttgagaaagg aatgatcttg cgtgatttgc tcgatgtccc ccaccccgat
    21421 ccgataacac cgactatcgc ccacgtgggc cacgtgaatc atcccgtccg atgataacac
    21481 cacagccacg atggttgttc ccatgccctt tcgatctggg ttttcctgcg ccgcccgaac
    21541 gacctgttca ttggcatgcc gtatggccaa caccaagcgc accgcatccg gcaccaagtc
    21601 gggatactcg ggaagagtaa gctcactgtc tgggctatca cgagtggctt caaaaaaatg
    21661 ttccacggaa ttcgccgcca acgcactcgc cacatctccc gcggaatggc cccccatccc
    21721 gtcggcaacc aaaaatagtc ctagatccgg cctggatagt actgtgtctt cgttgtgctt
    21781 gcgcttacgc ccaacgtccg taacggcagc accatggaac ctcacctccg aggatttctt
    21841 tcgagaatca tctgtcatga gcgcgtcgat atcaaccgca agtcaataac gcctcgaaag
    21901 gcggtttgtc cagatgtagg tggcaatata gcaagggccc cccgtcgagg ataggtcttg
    21961 aaaccacgcg atcgacgccc ataatcacaa ggctagccaa acgactgaat cgatccgtga
    22021 tacggttgtc ccgatgcgaa aagttcagcc ttccaccgtg actatcaccc gaccgcgttc
    22081 cgattccatg cacgtggaaa ccgcaacgtt tcgcctcacc gtggtgagcg ggccaaacca
    22141 aggacaagga ttcgaattgg atggcttggc cccatcccgc gttctagccg gcacgagccc
    22201 agcctgtgag attcgattgt ccgacccttt gatttcccga cgacattgcg cgatcgagca
    22261 aaccggacga cggctccgaa tcgctgatct tggttccacc aatggcacgt tcgtcaacgg
    22321 cgtggaaatc gtcgaggcat ggctcgatgg tggagaaaac ctgctcgtgg gcggcacgac
    22381 gtttcatgtt cagaaagacc ccaccgccaa aacagccttg ttgcccgcac gaaccatgtt
    22441 cggcagcgtg gtgggagcca gcgaagagat gcgaaaactc tatccgctgt gcgagcgact
    22501 tgcggcatcg agcgtccccg tgatcatcga aggagaaacg ggaacaggca aagaagtgct
    22561 ggcggagtca ttgcatgccg aaggaccgcg agccaacggt ccctacgtgg ttttcgattg
    22621 caccgccacc ccaccggatc tcatggaatc cgctatattt ggtcatgaaa agggggcgtt
    22681 taccggtgcc acggacacaa gagccggcgt attcgagcag gcggatgggg gaacgctgct
    22741 gttggacgaa attggcgatc ttcacatcaa cctgcaaccc aagttgcttc gcgtcattca
    22801 aagcatggag gtgcagcgcg tagggggagc ccgacgacga aaagtcgatg ttcgcgtctt
    22861 ggccgcaaca cgccgtgact tggatagaga ggttcaagcc ggccgatttc gcgatgattt
    22921 atactatcga ctggccgtgg ctcgcatcga gcttcctccc ctgcgacgac gtcgaggtga
    22981 tatttccctt ctcgccaagt acttttggtc cgaaaacgaa aacgctcttc ccatccccac
    23041 ggaattgctg caacggctcg aagactacga atggccggga aacgttcgag aactcagaaa
    23101 tgtcgtcttg agacaccaag ccctcggtga cctggctcga ttcgaacgcc ctctgatcgc
    23161 cgacagtccc gatgcactca tggctttttc tgatcccgtg ggtgagatca tccatcggct
    23221 tcttcctctt gcagaagcaa gacaagaggt catccaactg tttgaacagc gctatctcga
    23281 gcatatactc gcggtaaacg atggaaatgt cacgcgcgcg gcggccgctg ccggtgtggc
    23341 tcgacgttat tttcacttac ttcgcgccaa atatgccgcg aaaaacacgc cccacgagtg
    23401 atgcctcatg agcaatgata aacacggtag atgatgccat cctccgtacg ctcagcataa
    23461 accacaccgc cctcatccaa gaccaccgcg tcccgaaatg tctcgtctgg cagtgtattc
    23521 gctggcatcc atgcgccgcc caccggcgcg ccttgcgtgg gctccagaca gaccagttga
    23581 acacgaacat ctccaggaga aagctccgga ttgccaatga ctgccgctac gtaaatcgtc
    23641 ccatgcttgt cggaatcaag gagcaccaag tacatcaccg cttcttcaag acgcagttcc
    23701 cgcgtgaata gatggtctcg cgtggcccgc tgaatcgccg atacgaaaat cctccccgcc
    23761 tccgggtcta tgattcccgc cgataatagc aacgaaccat cacgcgaagg cctcccaggt
    23821 agctcctccg gctcctgcgc gggcgcccca tcttttgtcc ccaccttcac aagagcaccg
    23881 tgctcgcgct ccacatatac gtcgtcccca tccacgaaaa cacccgtgat gcctccaggc
    23941 tcgtcaatcg acgttccctc gagcggaatc tgccccacca cgtttccgtt gggatcgagc
    24001 accgtcacgt tcttatcctt gagccggtcg agaactagcg tcgttccatc actcgccacc
    24061 gtgacgtcct gggggtacag agcatcgagc cgagtatcaa acgtccgtct tccgtttttg
    24121 tcgtagcgag caaggcgacc attgacttgg tcgagcacgg tgagggtccc gttgggccca
    24181 atagcaaggc tcatcggtcc ttctggattg gcttcctcgg gaacatctcg accgatttga
    24241 tctttccccc ctccccacaa agccgatgca atcaccccgg ccgagggcgc agaggcatcc
    24301 tcacgcacca tcgtcggcgg agccacagaa agcactggtt cagggttcga cggcctatcg
    24361 accgtacgct ccaccggctc ggcgggccgt cttgtcgacg tcgttttcgg tggtgactcc
    24421 cgaggccata accaccatgc cgcaatggca agagcaagga gcgacaatac cgcgatgagc
    24481 ctagttctat tggcttgcat gatctttcgg tgacctacgc cggaagaata tcgccgacga
    24541 cgattggagg acagaagcaa aggactgaag caagaccttg accagtgcgg ttcatggagg
    24601 ttggcgattt cgtggcacat aaatcgcatg acaattcctt gaaatccaat cggaggagct
    24661 gctcatgtgg tacaaagtcg ctggaagtaa gtcgttctgg gttacgctcg ctgcaatgag
    24721 cgcggcgctc ttgatcatcg tgggagcttg cagtccagcc gctcccactg actcctccaa
    24781 cctcgaaagc gtcgacgaaa ccgtcgcatc gagcgaagaa gggctgtccg gttccttgcc
    24841 caccggcagc attctcaaga ccaccgcgaa cctgaacttc cgcacgggac catcgaccgg
    24901 aaacaaggtc att
//
LOCUS       DAOSOR010000013        23900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_2132_length_23900_cov_4.770686, whole genome shotgun sequence.
ACCESSION   DAOSOR010000013 DAOSOR010000000
VERSION     DAOSOR010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 23900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23900
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2132_length_23900_cov_4.770686"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..6964)
                     /locus_tag="PK710_01370"
     CDS             complement(<1..6964)
                     /locus_tag="PK710_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOT08381.1"
                     /translation="MTRSSFWISAVPRLLLGLLLLVLPLGGMACGDGDDEPSEPGVTL
                     KSSAISPATANIAVGATQQFAVNGVYSDGKTKPVKTGVTWTSSSTTVATINTAGLATA
                     VGDGTTTITAAVGKLKATASLTVLTKALTGVQITPASASIGVGTTQQYAATANYNDGT
                     TSDVTATATWASSDAAVATISATGLASGVAAGNTTISATFEGTTGTALLSVTAKTMTK
                     IVVTPATAEIAVGVNQPFVAMAHFNDNTTQVVTTEVTWESSNTKVATIASNGTATGVG
                     AGETTITATHSAGPSGSAKLTVKAVGPTELSVSCTDVKLAAGVTTQCKAVATMDDGST
                     ADVTSQATWKSDKDTVATVSNAGLVTAKGEGKANISAEYQGLSGSVEIEVTAAVLAAI
                     QVTPANAVVALGTTQQYTATGVYSDATTHDITDDVNWISSDLTVATIDGDGLATTLKA
                     GQTTISATLAGVTGTATLTVTGATPTSVVVSPATATTATGGTVQFTAMVHFSDGTQQN
                     LTSAASWSSSNDTIATVVGGLATGVAKGTATITATYSGQSGTASLEVTDATVTSVVVS
                     PASASIHKGGTQQFEAVAHLSDGTNTGVLTTGVTWVSDNTSVVTINADGLATGLAVGS
                     AHIRATYNSVQSALVPITVGNAVPTSLTITPPSAEIAKGTKATFTARATLSDGTVQDV
                     TSAANWSSSNTAVATVDKGVATGVEANANPVTILAEYQTLTATASLTVKAADLTAITI
                     SVSNAKVPVGSQITFKAIGSYTDGTSQAISDLVNWNQNPAGRLSFANGGTADSKLATG
                     ATVGTTSVTATLNGVTSNALSVEVVPVLLQSISITPAITQNKYVGETVQFNALGHFDD
                     GSSHDITADATLTWVSSHASVTISNGTNKGRATAAAVGSANITARVGAIVSPATQVNV
                     LAQPTIVAIQLEPTSWETSIGGSETFWATQINQDGTSGYLTDGVWTIEDANGAIVTNL
                     SIDLSVPHAAIIVAVSPTTNLPGGVAYVAVAKGLIKTRAPVKVKATTITSIAVNCPAP
                     NTCLPTGVGYLANCTAQATYSDGTTGDVTKTATWSVTTSPGTGVALALPQGEIQIGST
                     AGTANVFATMGSVTNATPAVLTSRARTISSLSAGGNFSLAMGDYSDRKATATYQALTG
                     CSDNTFDVTKFADWSSSNTAVATVDNSANKGRVQAVASGQASIQASFSGIQNIAIATV
                     SAACIKEIEIQVVEEAPANVTVPIVAMAELSDGSSAPLPVAAGSWGTAPVTNGYLDVG
                     TSDIDITYTYTGSSLCAGVPNTATAKLTIDKDAVLQSVAVEPSTSTITIGGHQDFVAS
                     AHFDSGTFNVSRFAVWTHAPAFPLSQAASPSAVPARRFTHTGTVGGSTNVLANYKAMA
                     SNPAVLTVSGAAPTAIEVRRADFVPAPVGTNYAYPPNFTVTTVARVKYSDGTEIDNPT
                     CVSWQSSHPGYLNFTNPNAPTATTYSNTTAQTVTITATCGTLSATATVQVIPAVLDEF
                     RFNPDSPVTRPRNTSFPLTVEGRYGTSWWDVSSMVSISNPPSGIASATVVSGKVMVQT
                     GANAGTATISFTKDTITRGYVVNVDTSNCLDSIEFTAPTADPVSLGVNQTVDFQARAH
                     YTNGTTGVLTTGTWTPSLTGDGVLIRTTDSGNTRTFRGVAVGESKVTFTYSGSNVCAG
                     GNTAINTITIERDVNVIQKVASAITIRPEPVSGQSRRRIPRGQDIQLSAWLSFTDPAI
                     PAEDVTESVTWSVTPSSIAGITAGGLLKGNAVGTGEAKAVLGSLTATLGIDVVACGNP
                     ALSITSPSAVALPIGQTRDYTASASYTCSGLSASELGPFPVTTLVTWNSSVPASAAFE
                     TSAFPNRITAKAAGSTNITAQYNGATSNTIGLSVVAVTLQSLTITSSGPMYVGGERDI
                     NVSAVWRAGTTDYNLDPPPALTWVIGDPTAISITATADPKKYILRGLAAVADITYRAR
                     HDTINSNTLTVSVANTCINEVRYAVGDRTMPKGISGGLKLECQNSAAPGVWDECPATG
                     TASFVSSDSKVVSVTGATFKVVGAVNEYADITGTYTGTGACTGVTLTDEARITVGSAK
                     VASIVVNGPDAVAKNHTAQYAASATFTGGTGAGTYNIAADAIWTSTNEAVATVSAGVL
                     TSKNVNGTTYLSATFEGISSPLKLVTINDKVPTKITVWADWNLVSENGGPAVPATGAD
                     AEYPTGNWKLQLHGTVDYSDGTSDDDPAGVTWVTRGTLLKDVSVTGGLFNTGDTANDA
                     LQTVRATYTYNGVTVDDDFTVRMRRSTATSTIVIRNTTNTGAGVASVPLGLTQPYGAR
                     LTYGGRYYWFTRNAT"
     gene            complement(7485..8837)
                     /locus_tag="PK710_01375"
     CDS             complement(7485..8837)
                     /locus_tag="PK710_01375"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT08382.1"
                     /translation="MRLVIIGGDAAGMSAASEARRRAPEAEIVVLEASRDVSYGACGL
                     PYKLVPGADVEDLNIISAERFRTERALDVRLGHQVTSVDSAGKMVRGQADTGPFALSY
                     DKLLIATGASAVRPAIDGLESLWGKGAYVLKTLQDGRELKAALKDKPRHAVVVGGGYI
                     GLEATENLRKAGLEVTVVEALPSLVPFVPEAMRQRVMQEAASQGVSIRLDTKVVEVRR
                     GASGRMVVGTTGGAIETDLVLVAVGVRPNVAFAKQAGISLGAGGAIAVDDMLRTSVED
                     IYAAGDCADAKHVVTGKSVWIPLALRANRAGKVAGQNMTGSRTTIPGIVGTAVFKFFD
                     LEIARTGLSEQEAKEAGWDVVVATITSSTRAHYYPGGGKLFVSLVAERKTGKLLGGSM
                     VGPEAAAHRVDSVAVALHAGLGAKELYAMDLAYAPPFGPSWSPLLIASSALMKAMKVD
                     "
     gene            complement(9501..10964)
                     /gene="guaB"
                     /locus_tag="PK710_01380"
     CDS             complement(9501..10964)
                     /gene="guaB"
                     /locus_tag="PK710_01380"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOT08383.1"
                     /translation="MIDYPIREALTFDDVLLVPAFSEVLPTQVDVRTRLTRRISLNLP
                     IVSAAMDSVTESRCAITMAREGGLGVIHKNLSIEQQAREVEKVKRAESGMIMDPITAR
                     PDQLLRDVLELMREHDISGVPVVQDGKLVGILTARDIRFERELDQPVHALMTRDLVTV
                     PAGVAPEQARDLLHKHRIEKLLVVEDGKLLGLITIKDLMHAERNPNAVKDELGRLRVG
                     AAIGPGADWRSRAEALVAAGVDVLVVDTAHGHSKGVIDTVGECKKRYPQVDLIAGNVA
                     TAQATQALIDAGADAVKVGIGPGSICTTRVVAGIGVPQITAIDDCARVGDKHQVPIVA
                     DGGIKYSGDVTKAIAAGASSVMIGSLFAGTEESPGQLVLFQGRTYKSYRGMGSLGAMR
                     QGARDRYGQSESADDKLVPEGIEGRVPFRGALHSVLYQLVGGLRSGMGYTGCRTIEEL
                     RRQATFIRITGQGLRESHVHDVIITEEAPNYSGRPDR"
     gene            complement(11004..11894)
                     /locus_tag="PK710_01385"
     CDS             complement(11004..11894)
                     /locus_tag="PK710_01385"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT08384.1"
                     /translation="MVLSEGDRLDARYRVTSLIGRGGMGEVWAGQGDQGPVAIKVLLE
                     RSARRKDIQRRFEREAEAVSRIQSPFVCALLDKGLTSDGSLFLVFELLQGESLADRLR
                     KELFLPFAELGPLMADAWQGVADAHRANVLHRDLKPGNIFLQPGKQRENAVLLDFGVS
                     KVLQDVRASQEPSITAYDGTVGSFAYMAPEQIRGAARVDERADVYGLASVAFRALTGR
                     LPFEGLSARMVASLKLDNPPPTLEQATGFRWPVVLEEFFGIGLSRNPDDRFASADEAK
                     RRWTEACECFDHARDAKNLG"
     gene            12009..12404
                     /locus_tag="PK710_01390"
     CDS             12009..12404
                     /locus_tag="PK710_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CesT family type III secretion system chaperone"
                     /protein_id="HOT08385.1"
                     /translation="MAKSQRDVEAYLQALGKSWDKLEDGTYIIHTSNEIPIALRITTS
                     LLVARVLIGAIPNTNAQHQVQLFRRLLELNASELMYASYGLEDGNMVLSAALELENLD
                     MNELEAVLGDIDLALARHVSELWELCKEA"
     gene            12442..13278
                     /locus_tag="PK710_01395"
     CDS             12442..13278
                     /locus_tag="PK710_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HOT08386.1"
                     /translation="MGIFARLAQLIKSNINDLISRSEDPEKMLNQIVIDMNNQLAEAT
                     KQVAVAIADEKRLSKHVEQEAANAAEWKRRAEMAIRAGNEELAREALARRKEHEQLRV
                     GFEQQWQKQKAAVDQLKRALRMLNSKIEEAKRKKNLLIAQKQRAEAQKAIHQAMGGVR
                     DNSAFETFDRMADRINRMEAEAEASAELAEEYSGDVLKSKFDELEQTAGADMELLELK
                     REMGLIPPEPQPAQVRVQPVVEAPPATASLTSEQQEQDELAAALAELEAEELQAQRKM
                     QR"
     gene            13337..15235
                     /locus_tag="PK710_01400"
     CDS             13337..15235
                     /locus_tag="PK710_01400"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOT08387.1"
                     /translation="MSLPRWLCPTLLSLAVMISASARADVPLFLAKPGEIKVDGVLRD
                     WGGTPFFRLDSRVQGSSPSNVRAALAYDATFLYVAAQVVDPSFVRTNRFLDTEDHAEL
                     LLSFPNAQGQYNRVFEVKLFAGKPGETEGQVKAVGLGPVRGAEIVEAPSQGGYTFEAK
                     IPWSVFPPAAQTRVKLRGALRYLDGDGRGTSAIIASAPSNRAADLPLLLSAPEQALVR
                     GLINDRKFGSPTADIIANVAGDSMTERVLLFHNTLVVYGPTFRKGTEYFWSDITDPNA
                     GQLPLFDVRDLTGDGKSEIIVVKRVSRGTGWRQMLQVLSFRGDSLQPIFEHETGLGSS
                     QGTIENKVSLVPSGQGHSIVISLGSDSGYDASTYHEPQETEREPLLLPWGTVQSRTYA
                     WDGKQFVKRSEQTKAADSAPARPAAPPPPRPPSADELLDQVYRLYKKDHNIPEVAKPT
                     FDFVTNVAASQEVERIICHGRDLVVFGKGFREGRGYVSLSMPQFVSDKDIHDVSAFDV
                     TGDGLADIIVRGSQRTKAPEELGQGELVREVLFVYRVDESSISRVFAAETAVTLGSRR
                     IYSLLAFVPSANGLDIEIRPGRAIGWTRATWPYRQDTEAVSGLEPIVLPWTEQPFRFR
                     FAGQTFSR"
     gene            15312..16136
                     /locus_tag="PK710_01405"
     CDS             15312..16136
                     /locus_tag="PK710_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08388.1"
                     /translation="MARLSALRSSDPREDALDSIEVPAAVVCARCGKPDCPGCDSLDE
                     TTLPSGVISIVPWERPGTSAWSRLWLTARAATRSAASFFQSLPPGSHTSAFSFSLIAE
                     FYAVVSWAVFISLLVALPLHDIAWLMLKNPAGRAMLARLWVVGTVGFTVVLVVGHAIY
                     GVALDKGARRTGARSRFSQALRFGLYASGWDLLTSPLGLLVSLIAEGPRATIHLLTAS
                     MGLPRRAATAFLRGIYHLEGPPLAQARNYGTTVAIILSITGAIVVLVSLVFAALGF"
     gene            complement(16179..16325)
                     /locus_tag="PK710_01410"
     CDS             complement(16179..16325)
                     /locus_tag="PK710_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08389.1"
                     /translation="MLSQDAMGALVFFDVLSVFLVLVDRFAFAARRDRDRDRGRERGR
                     ERVP"
     gene            complement(16642..17937)
                     /locus_tag="PK710_01415"
     CDS             complement(16642..17937)
                     /locus_tag="PK710_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08390.1"
                     /translation="MRRLWLRGRIFTPVLVLAALVGVASSCSSEEEADESTPHAGAGG
                     SSAAGGSGGSQSGGSGPTAGSAGSTGGSGNAAGNAGSAASAGSGASAGSTDGGAKDVS
                     FDYQPPDVTEPDACAAVTAEAVKPPVDIIWIIDQSGSMGNEISQVKANVNGNFANIIQ
                     NAGLDYRVILFANGTSGISATYRLCVDPPLGAAACGEDNPPHFFQVNRTIASTNSFSL
                     FMNDTYWTPIKANLRPSAFKAFIEVTDDQSSVSATNFDNFLLTGNGAGYFGTSAERNY
                     VFHSIIGVHAPLAPTEPTTNSKCSTAVNTGPQYQEISILTGGLRHPVCDTNYSAVFNN
                     IATSIVKAVACELLTPPATSDAGVIDWSRVTVQYTPSNGGAVEVFPLVDNEAQCVGDG
                     FYYDNPANPTKVRLCPASCAKVQADEGAKVELLLGCLGS"
     gene            complement(18189..18704)
                     /locus_tag="PK710_01420"
     CDS             complement(18189..18704)
                     /locus_tag="PK710_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08391.1"
                     /translation="MMVVSANQTWQVVFPSLPISLSMLQALPEASLKEPYYAAALVIP
                     ALCLWPTNREEAIQMINFLKGPQPLSKFDLQFIADRLRGNEYVPLSYFEGAKPENGYQ
                     PQSPHTVTVSTVPTSFATEGHAQLYLRSGGADSPRPVKLRRKPSTGQWFLTEQMLLAQ
                     IRKPTALDPWA"
     gene            complement(18900..19847)
                     /locus_tag="PK710_01425"
     CDS             complement(18900..19847)
                     /locus_tag="PK710_01425"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT08392.1"
                     /translation="MRLVSGRFANLDGSVPQTFSSVFKWAVLDRILGRRRRSASRASV
                     PVMAVDLERITTPPSVGARLTWLGHASWLVQLDGLSLLIDPVFSRSIGPGIARNVPAP
                     LAAHELPGIAAQLVTHNHRDHLDLPSLRAVGSPIITGPGHRALFGTAVETTELDWWEA
                     TDLGPVRITFVPAQHWSRRGLWDANRGLWGGFVVRGSSATIYHAGDSAWFDGFSGIGK
                     RFSDIDAALLPIGAYDPPWFMQKQHVSPEQAVRAFEELGARYFVAMHWGTFKLSDEPL
                     DEPPLRLEKEWERRGFAREFLCIPAQGQTFEIGRGRSLG"
     gene            complement(19877..21205)
                     /locus_tag="PK710_01430"
     CDS             complement(19877..21205)
                     /locus_tag="PK710_01430"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT08393.1"
                     /translation="MNDKATSSSKESGFRSFPRVFWVVVLMEFFERGSYYGLMSVLSV
                     YMVDQVADGGLGFSKGAAGAINSTIRPLLYFLPIVAGAVADRFGYRRSLLVAFCLLST
                     GYFLAGQMTSYVAVFLSLIVMATGAGIFKPIISGTIARSTTESNSGLGFGIFYWTINL
                     GAFLFPLVLVNYLRTHAYPWSAIFTASALGTGIMLIPTVFLYREPPKPESTKTLAQVL
                     GGAVLVLKDWRFVTMIAIYSGFWVLYFQMFDTVLWYLKAHVDMSPFDATINGVLQALG
                     FDGKFKFDSEHVTVINAGTIIVLQLIISSIVKNWRALPTMIGGIAIGTAGFILLAIST
                     SPWIFMAGIIVFSIGEMTAHPKFISYVGLIAPEDKKATYMGYSFLYGVIGSSVGGYFG
                     AKLYEHFVDGCHQPRTLWLIFVGLGIATILGLVGYDRFIALRPASPARSP"
     gene            complement(21202..21756)
                     /locus_tag="PK710_01435"
     CDS             complement(21202..21756)
                     /locus_tag="PK710_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08394.1"
                     /translation="MKRVSYFSWIVSASLAVACGPSASTTSPATPSTPTATEEQPPTV
                     VYSKFTDPLFLALGEEKNVFGRRTLARLWVVASNFDPPGPAQQARLQEAQRAVDEAEH
                     AVGAQTDVCEAGVSVAVVRAIETKKQVEQRYLSTVEQGGEACPQARSLDAQVHFLRDE
                     IEALQELAQPCPPQMFDDPPEESR"
     gene            complement(21768..23516)
                     /locus_tag="PK710_01440"
     CDS             complement(21768..23516)
                     /locus_tag="PK710_01440"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT08395.1"
                     /translation="MSTDEQDNKPDQDSEETKPNPFQEIVQPFIDLVHAPRALWGINA
                     AYFLEGMVYFGMLMYLAMYFNEYVGLSDSMAGIMVGVLTSGITFSMFLFGGLADRWGV
                     RIALIAAFFLMIGGRIALSGAPSVGMGSTGLGSPVHIIAMVGILLIVLGYGMYQPAAY
                     AGVRQFTTPKTAAMGFAMLYALMNLGGWVPTFFSPIRKAFGISGAFWVFTAASVVALL
                     LTIVLLTKKVVDRTTAEAKAEKEAQKSDEKKEQEAEKKEKENAALAEARARGFFAMAW
                     HWLRHHPLADPKFTFFIFALIPVQTLFAHNWLTLPLYVERAFAPPVGVEACDSLASSS
                     EALPALEGARTVEALKDVVLRFEGSKQACEAKKQIRSMAAVKLVEGDKKAAEQAEEAK
                     RTIRTLDFQAAAKESSAKGNWSETARLWLGENFEVAVNFNPLLIFILVPIVTALTQRR
                     KVYNMMIVGTFVMAGPTFLLVLGPTFWTLLGYLVIMTIGEAMWQPRFLQYAAEIAPEG
                     RTAAYMGVAQFPWFLTKMIVPLYSGYFLSKYVPDSGPVQSETMWLIYGIIAVSSTVFL
                     VLAKGWIGKDFKTQAE"
BASE COUNT         5258 a         7404 c         6432 g         4806 t
ORIGIN      
        1 aagtggcgtt acgggtaaac cagtagtaac ggccgccata ggtgaggcga gcaccgtagg
       61 gttgcgtcaa cccgaggggt accgacgcga cacctgcacc ggtgttggtg gtgttgcgaa
      121 tgacgatggt cgaagttgct gtcgatcttc gcatccgaac ggtgaagtcg tcatcgacgg
      181 tcacgccatt gtacgtgtag gtcgcacgaa cggtttgcag cgcatcattg gccgtatcgc
      241 ccgtgttgaa caacccaccg gtgacggaaa cgtccttgag gagcgtgcct cgggttaccc
      301 aagtcactcc agccggatcg tcgtcactgg tgccatccga atagtcaacc gtgccatgaa
      361 gctgtagctt ccagttgccg gtcgggtatt ctgcatcggc acccgtggct ggaacggcag
      421 gtccgccgtt ttcacttacg aggttccaat ccgcccacac ggtgatcttg gttggcacct
      481 tgtcattgat ggtcacgagt ttgagcggcg aggaaatgcc ttcgaaagta gcgctgagat
      541 aggtggtgcc attgacattc ttgctggtca aaacacctgc gctgacggtc gccacggcct
      601 cattggtcga ggtccagata gcatccgccg cgatgttgta ggtgccagct cccgtaccac
      661 cggtgaacgt agccgaagca gcgtattgcg ccgtatggtt cttggcgacc gcatccggtc
      721 cgtttaccac aatcgacgcc actttcgcgg aacccacggt gattcgagcc tcatccgtca
      781 gagttacgcc cgtacaagcg ccggtgccgg tataggtacc cgtgatatcc gcgtattcat
      841 tgaccgcgcc cacgaccttg aacgttgcgc ctgtaacgga cactaccttc gagtcgctcg
      901 agacgaacga ggcagttccg gtcgcggggc actcatccca cactcccggg gccgccgagt
      961 tttggcactc gagtttcaac cctccggata ttcccttagg catcgttcgg tcacctacag
     1021 cataacggac ctcgttgatg caggtattcg ctaccgatac ggtcaaggta ttggagttga
     1081 tcgtatcgtg tcgagcgcgg taagtgatat ccgccacagc cgccaatccc ctgagaatgt
     1141 acttcttggg atcagccgta gccgtaatgc tgatggcggt cggatcccca atgacccaag
     1201 taagagcagg aggtggatcc aagttatagt ctgtcgtacc cgctctccat accgccgata
     1261 cgttgatgtc tcgctctcct ccgacataca ttggcccgga agaggtgatg gtcagcgact
     1321 gaagcgtgac cgccacgaca ctgagaccaa tcgtgttgga agtggcgccg ttgtattgag
     1381 cggtaatatt ggtgcttccc gcggctttgg ccgtaatgcg gttcgggaag gccgaggttt
     1441 cgaaagctgc gctggccggt acggagctat tccatgtcac caatgtcgtc acagggaaag
     1501 gcccgagttc agacgcggaa agtccagagc atgtgtagga tgccgacgcc gtgtaatccc
     1561 gtgtttgacc aatgggcaga gccacggcag aggggctggt aatggaaagg gctggattcc
     1621 cacaggcaac cacatcgatt ccaagagttg ccgtcaagga ccccaatacg gcctttgctt
     1681 caccggttcc gactgcattc cctttaagca gtccgccagc ggtaataccg gcgatcgacg
     1741 aaggagtcac actccaggtc accgactccg ttacgtcttc cgcgggaatc gcaggatccg
     1801 tgaaggagag ccatgctgag agctggatat cttggccgcg cggaatgcgc cgacgcgact
     1861 gtccagacac cggctccggc cgaatggtga tcgccgaagc aaccttctgg attacgttca
     1921 catcccgttc aatcgtaatc gtgttgattg cggtgttgcc gccagcacac acattcgacc
     1981 cggaataggt gaaagtaacc ttggattcac caacagcaac gccccggaag gttcgcgtgt
     2041 tgcccgagtc cgtcgttcga atcaggacac cgtcacccgt caagctgggg gtccaggtac
     2101 cagtggtgag cacacccgtc gtcccattcg tgtaatgagc acgggcctgg aaatccacgg
     2161 tttgattgac gcccaacgaa acggggtctg ccgtgggggc cgtaaactcg atactatcga
     2221 gacaattgga agtatcgacg ttaacgacat acccacgggt gattgtgtcc ttggtgaaac
     2281 tgatggtagc cgttccagca ttggcaccgg tttgaaccat cacctttccg gaaaccacag
     2341 tcgccgaagc aattccagac ggcgggttgc tgatggacac catcgaagag acatcccacc
     2401 aggaagtgcc gtaccttcct tccactgtca gggggaaaga agtatttcgc ggacgcgtaa
     2461 caggcgaatc tgggttgaat cggaattcat ccaatacagc cggaatcacc tgcaccgtgg
     2521 ccgtcgcgga gagggtcccg cacgtcgccg taatcgttac cgtttgtgcc gtcgtattcg
     2581 agtacgtcgt tgccgtgggt gcattcggat tcgtgaagtt caagtaaccc ggatggctcg
     2641 actgccaaga gacgcacgtc ggattgtcaa tttccgttcc atccgaatat ttgacccgcg
     2701 ccacggtggt cacggtgaaa ttcggaggat aggcgtaatt ggttccgacc ggagcgggaa
     2761 cgaagtcagc ccgtctgacc tcgatggccg taggcgcagc accagaaacg gtcagtacgg
     2821 ccgggttcga tgccattgcc ttgtagttgg ccaatacatt cgtggaacct cccaccgtac
     2881 ccgtgtgggt aaaacgtcgg gcaggaaccg cgctgggaga tgcggcttga gacaatggga
     2941 atgccggagc atgcgtccaa accgcgaatc ggctcacgtt gaaagtgccg ctatcgaagt
     3001 gggcactagc gacgaagtcc tgatgtccac caatcgtgat agtggaagtc gacggttcga
     3061 cggcaacact ctgtaggaca gcatccttat cgatggtcag cttcgccgtc gcggtattgg
     3121 gcacgcccgc gcaaagcgag cttccggtgt atgtataggt aatatcgatg tcggatgtcc
     3181 ccacatccaa gtagccgttc gtgactggag ccgtgcccca agaccccgcc gcaaccggaa
     3241 ggggagcgct cgatccgtca ctgagttcag ccatcgccac aatcggtacg gtaacattcg
     3301 cgggggcttc ctctaccact tggatctcga tttccttgat acaggcagcc gaaacggtag
     3361 caatcgctat attctggatt cctgaaaacg atgcctgaat actcgcttgc cccgaagcga
     3421 ccgcctgaac acgacccttg ttcgcactgt tgtccaccgt ggcaaccgcg gtgttgctcg
     3481 acgaccaatc cgcaaacttc gttacatcga acgtgttgtc cgaacatcct gtcagcgctt
     3541 gataggtggc cgtggctttg cgatcgctgt agtcgcccat cgccaacgaa aagttcccgc
     3601 ctgccgaaag gctgctgatc gttcgagccc tggatgtcag gaccgcggga gtcgcgttgg
     3661 taactgagcc catcgtagcg aaaacattgg cagtaccagc ggtagaacca atctggatct
     3721 ccccttgcgg tagcgccaag gcaactcccg ttccgggtga tgtagtcaca gaccaagtag
     3781 ctgttttggt aacatcaccc gtcgttccat ccgaataggt ggcttgggcc gtgcaattcg
     3841 cgaggtaccc aacgcctgtc ggaagacagg tattcggtgc aggacagttc accgcaatgg
     3901 acgtgatggt cgtagccttg accttcaccg gcgcacgcgt cttaatcaag cccttcgcca
     3961 ccgcaacata cgcaacgccc cccggaaggt tcgtcgtagg cgaaaccgcc acaatgatgg
     4021 ccgcgtgcgg aaccgacaga tcgatagaaa gattcgttac gatcgcacca ttggcgtctt
     4081 cgatggtcca gaccccatcc gtcagatacc ccgaggttcc atcctggttg atctgcgtcg
     4141 cccagaaagt ttcgctccca ccaatgctgg tttcccaaga ggtgggctcg agttggatgg
     4201 ccacgatggt gggctgcgca agaacattga cctgcgtcgc cggcgatacg atcgcgccaa
     4261 cacgggctgt gatattggcg gaacccacgg cagccgccgt ggctcgtccc ttgttggtcc
     4321 cattggatat cgttacggat gcatggctcg atacccaggt caacgtcgcg tcagccgtaa
     4381 tgtcatggga cgatccgtca tcgaaatgcc caagagcatt gaactgaacc gtctcaccga
     4441 cgtacttgtt ctgggtgatg gccggagtaa tggagatgct ctgcaacagg acgggcacga
     4501 cttcgacgct caaggcgttg ctcgtgactc cgttgagagt agcggtcacc gatgtcgtgc
     4561 cgacggttgc acccgtggcg agcttgctat cggccgtgcc tccgttcgca aacgacaagc
     4621 ggccggcggg attctggttc caattcacaa ggtcgctgat ggcttgagaa gttccatcgg
     4681 tgtaactgcc gatggctttg aaggtgattt gtgaaccaac aggaaccttg gcgttcgaaa
     4741 cacttatggt gatggcagtc agatccgccg ctttgaccgt caaggaagct gtggcggtca
     4801 gcgtttggta ttcggccaag atggtcacgg ggttcgcgtt ggcctcgacg ccagtggcaa
     4861 ctcccttgtc caccgtggca accgcggtgt tgctcgacga ccaattcgcc gcggaggtca
     4921 catcctgcac ggtgccatcg ctcaacgttg cccttgccgt gaaggtagcc ttcgttccct
     4981 ttgcgatttc ggccgaaggc ggagtaatcg tcagggaggt aggtacagca tttcccaccg
     5041 taatgggcac caacgcggat tgcacggagt tataggtcgc ccgaatatgg gcggagccca
     5101 cagccagtcc ggtagcaagc ccatcggcat taattgtcac cacggaagtg ttgtccgaaa
     5161 cccaggtgac gcccgtagtc aacacgccgg tattggtgcc atcgctgaga tgcgcgacgg
     5221 cctcgaattg ctgggttcct cctttgtgga tgcttgccga tgcaggggaa accaccaccg
     5281 aggtcaccgt cgcatcggtt acctccaacg acgccgtacc ggattgcccg gaatacgtgg
     5341 cggtgatggt ggccgtgcct tttgcaacgc ctgtcgccaa accgccaacg acggtggcaa
     5401 tcgtatcatt gctcgaggac caagaagccg cagaggttag attctgctgc gtgccgtccg
     5461 agaaatgaac catcgccgtg aactgaaccg ttccacccgt ggcggtggtc gccgtagccg
     5521 gcgagacgac cacggaagta ggcgtggcac cggttaccgt cagcgttgcc gtcccggtga
     5581 cacctgcaag ggtggcggaa atggtcgttt gtcccgcctt gagggtggtg gcaagaccgt
     5641 cgccgtcgat ggtcgcaacc gtgaggtcgc tggaaatcca gttcacgtcg tccgtgatgt
     5701 cgtgggtggt cgcatcacta tacacaccgg ttgcggtgta ctgttgcgtg gtaccgagag
     5761 caaccacggc gttggccggg gttacctgga tcgccgccaa taccgcggcg gtgacttcga
     5821 tttcgacgct tcccgacaat ccctggtact cggcggagat attcgctttg ccttcgccct
     5881 tggccgtgac gaggcccgca ttggatacgg tagccacggt atccttgtcg gatttccaag
     5941 tcgcctgcga ggtaacgtcg gccgtggatc catcatccat cgtggccact gctttgcact
     6001 gcgtcgtcac gccagcggca agtttgacgt ccgtgcagct cacactcagc tcggtgggac
     6061 caaccgcttt gaccgtcagc ttggcggaac ccgacgggcc cgcgctatgc gtagcggtga
     6121 tggtcgtttc tccagcaccg acgcccgttg ccgttccatt cgaggcaatc gtggcaacct
     6181 tggtattcga agattcccac gttacttccg tcgtcacgac ctgcgtcgtg ttgtcgttga
     6241 agtgagccat cgccacgaac ggttggttca cgcctaccgc gatctcggcc gtggcagggg
     6301 tgaccacaat cttcgtcatc gtctttgccg tcaccgaaag cagcgccgta ccggtcgtgc
     6361 cttcgaacgt cgcggagatg gtcgtgttgc ctgcggcaac gccggaagca agaccggtgg
     6421 cgctgatggt agccacggca gcatccgaag aagcccacgt cgcggttgcc gtcacatcac
     6481 tggtcgtgcc gtcgttgtag ttggccgtgg ccgcatattg ctgcgtcgtg ccgaccccga
     6541 tcgaagcgga agccggcgtg atttgcacgc cggtgagcgc tttggtcaag acggtcaacg
     6601 aagcggtagc cttgagtttg ccgaccgcgg cggtgatggt cgtggttccg tcgcccaccg
     6661 cggtggcaag accggcagtg ttgatggtcg ctaccgtggt agaactggaa gtccaggtca
     6721 cgccggtttt tacaggcttg gtcttgccat cgctgtacac accgttgacc gcaaattgtt
     6781 gtgttgcgcc gaccgcgatg ttggcggtgg caggactgat agcacttgat ttgagggtga
     6841 ccccaggctc ggacggttca tcgtcgccgt cgccgcaggc catccctccg agaggaagaa
     6901 cgagtaataa aaggcctaat agtaatcgtg ggaccgctga tatccaaaaa cttgaccgtg
     6961 tcattccttc acctccaaga caatttcaag tctcctaacg aagcgaggca accaatccat
     7021 ttccggcgtt cgaacaagcg gcgaagctcg ttggttaccg ccatagccat agctgataga
     7081 ccccatcatg ttatgcgcct ccttgttgcc tcccagagcc atgttggaaa ccccggcacc
     7141 ccacgggagg tgccttccaa gcgaaagtat gaaacacgag tcgaaacggt gagtcaagcg
     7201 ctgatacgac agggttcttt caataaaata tgtctaggac gcaatatacg aaacctgctt
     7261 gaacaataag gtctgagaga agcgaacggt ccgatccatg aatattttta ttgtatttcc
     7321 attgttttca acttattgag gccttggttt cgtccgggtc ccgacctccg caccgatacc
     7381 tctagcgcgc caccaaacgt aaatgaaaat gcggcgtgcc cgccctgccc acgccgcgtt
     7441 gtcaacccca acaatcggta ccggaaatac ttttgcctcg aagctcaatc cactttcatg
     7501 gccttcatca aagcactgga ggcgatcaac agcggggacc acgaggggcc aaacggaggc
     7561 gcatacgcca gatccatcgc atacaactcc tttgcgccca agcccgcgtg cagcgccacc
     7621 gccacgctat caactcgatg cgccgccgcc tccggaccca ccatactgcc cccaagcaac
     7681 ttgcccgtct ttcgctccgc caccaacgat acgaagagct tcccgcctcc cggataataa
     7741 tgcgctcgcg tcgacgacgt gatggtggca accaccacat cccaccctgc ttctttggct
     7801 tcttgctccg ataatcccgt tcttgcgatc tccagatcaa aaaacttgaa taccgcggtg
     7861 cccactatcc cagggatggt cgtccgggaa cccgtcatat tctgccccgc aaccttgccc
     7921 gcccgattcg ctcgaagcgc caacggaatc cacacggact tgcccgtcac cacatgcttg
     7981 gcatccgcac aatcgcccgc cgcataaatg tcttccaccg aagtacgaag catgtcatcg
     8041 acggcaatcg ctcctcccgc tcccaatgaa atccccgctt gcttggcaaa cgctacgttc
     8101 ggacgtacac ccaccgcaac gaggaccaaa tccgtctcga tcgcaccacc cgtcgtcccc
     8161 accaccatcc ttccactcgc tccacgacga acctccacaa ccttcgtgtc cagccgaatg
     8221 gataccccct gcgacgccgc ctcctgcatc acccgctgcc gcatcgcctc cgggacaaac
     8281 ggcaccaacg acggcaacgc ctccaccacc gtcacctcca acccagcctt gcgcaagttc
     8341 tccgtcgcct cgagcccaat atatcccccc cccaccacca ccgcatgacg aggcttgtct
     8401 ttcaacgccg ccttcaactc ccgaccatcc tgcaacgtct tcagaacata agcgcctttt
     8461 ccccaaagcg attccaaccc atcgatggca ggacgaaccg cgctcgctcc cgtcgcaatc
     8521 aaaagcttgt catacgacaa cgcaaagggg cccgtatccg cctgccctcg aaccatcttg
     8581 cccgctgaat ccacgctcgt gacttgatgg ccaaggcgaa cgtcgagggc acgctccgtg
     8641 cgaaaacgct ccgcggagat gatgttcaaa tcctccacat ccgcacccgg caccaattta
     8701 taaggaagac cacaagcgcc gtaggaaacg tcccgcgatg cctccaacac cacgatctcc
     8761 gcctcgggcg cacgacgtcg cgcctcgctc gccgcactca ttcccgccgc atcgccaccg
     8821 atgatcacca gacgcatgga cattctcctt tcaaccccga ttgatccttc gggctttgtc
     8881 tgtaaccacg acatcaccga aacgccaccc cacgatcgca acctgctttc cacccacctt
     8941 tccctccttc gcaataaaca ggaaatgtcc cgagacacag gcaggcaacg aggagccgta
     9001 atcctagtaa gatattgaat tacttaggtt tttatattct ccgtaagatg acgaattcac
     9061 ctcacgtccc ggcggccccc ccctcgcaag cacccatcgc tcgccctctc atttaaccca
     9121 tgctcgcgta tcgcatttcc aggccgaccc catccatccc cactccgttg ccaacccaaa
     9181 ccccgagcgc aaccacggaa cgatatccag atagacccat gaacggcagc gccaacccat
     9241 ggtttcaaaa catatatttc gcagtttccc caaattatct ccgcagtcat gatgattccc
     9301 gttaattatc cgtcttcgga tatatcggac gcttcctctg ccatcgctcg ccacccctca
     9361 cagcgcaacg cgcaacgata ctcatgacgt cgacccctgc cgtctctgct ctgcgcatcc
     9421 gcgccatgga tcgcccgtca taaaaatctt caccaactcc gcgctcggct cacgtcccac
     9481 gcatacgcgg aaaccgagac tcaacggtct ggacgccccg aatagttggg cgcctcttcg
     9541 gtgatgatca cgtcgtggac atgagactct cgcagcccct ggccggtgat acggatgaat
     9601 gtcgcctgcc ggcgaagctc ttcgatcgtc cggcaccccg tatatcccat gccggagcgc
     9661 agtcccccca cgagctggta gagcacggaa tgtaacgcac cgcgaaaagg aacccgtcct
     9721 tcgatcccct ccggaaccaa cttatcatcc gcggattcgc tctgaccgta gcgatccctc
     9781 gcgccttgac gcatcgctcc caacgatccc attccccgat aggacttata ggtccgccct
     9841 tgaaacagca cgagctgtcc cggtgactcc tcggttccgg caaacagcga accgatcatc
     9901 acgctgctgg ctcccgcggc aatggccttc gtcacatctc ccgaatactt gattccgcca
     9961 tcggccacga tcggcacctg atgcttgtcg cccacccgcg cgcaatcgtc gatcgccgta
    10021 atctgtggca ccccaatacc cgccacgact cgtgtcgtgc aaatcgaccc tggtccaatt
    10081 cccactttga cagcatcggc acccgcgtcg atcaacgctt gtgtcgcctg cgccgttgcc
    10141 acgttgcccg caatcaggtc cacctgggga taacgttttt tgcattcccc caccgtatcg
    10201 atcacccctt tcgaatgacc atgggccgtg tccacgacaa ggacatcgac tcccgcggcg
    10261 accagcgctt cggctcttga tctccaatca gcgcccggtc cgatggctgc ccccacgcgc
    10321 aaacgcccga gttcgtcttt gaccgcattg ggattacgct ccgcgtgcat caagtccttg
    10381 atcgtgatga gcccgagcag cttgccgtcc tccaccacga gcaatttttc gatacgatgc
    10441 ttgtgcagaa gatcccgcgc ttgctccggg gctacccctg ccgggacagt aacgaggtcg
    10501 cgcgtcatca acgcatgaac aggttgatcg agttcgcgtt cgaaacggat gtctcgagcg
    10561 gtaaggatgc ccacaagctt accgtcctgc accacgggca caccgctgat atcgtgctcc
    10621 cgcatcaact cgagcacatc gcgcagcagt tggtcgggtc gcgcggtgat cggatccatg
    10681 atcatgccgc tttccgcacg cttgactttc tcgacttcac gagcttgctg ttcgatcgac
    10741 agatttttat gaatgacgcc gagtcctcct tcccgcgcca tcgtgatggc acatcgggac
    10801 tcggtgaccg aatccatcgc ggcgctgaca atgggcagat tcagggagat gcgccgcgtc
    10861 aatcgtgtac gaacatccac ttgcgtgggt agcacttcac tgaacgcggg aaccagaaga
    10921 acatcgtcga acgtgagcgc ctcgcgaatg ggataatcga tcatgatgca cctgccctcg
    10981 ctcataacac ggtccgcgga gccctacccc aagttcttcg cgtcgcgtgc gtgatcgaag
    11041 cactcgcatg cttccgtcca tcgccttttc gcctcatccg cggaagcgaa gcgatcatcg
    11101 ggattgcgcg aaagcccgat gccgaaaaac tcctccaaca cgacaggcca tcgaaatccc
    11161 gtggcttgtt cgagggtcgg cgggggattg tccagcttca aactcgccac catccgcgcg
    11221 ctcaaccctt cgaagggcag ccgaccggtc aacgcacgaa acgccacgga agccaaccca
    11281 tagacatccg cacgctcgtc gacccgcgcg gcaccccgaa tttgctcggg agccatatac
    11341 gcaaaggagc ccaccgtccc atcgtaagcg gtaatggatg gttcctgact ggcgcgcacg
    11401 tcttgcagga ccttggacac gccaaagtcc aacaaaaccg cattttcccg ctgttttcca
    11461 ggctgcaaaa aaatatttcc cggcttcaaa tcccgatgca gcacgttcgc tcgatgcgcg
    11521 tcggccaccc cttgccaggc atcggccatc agcggtccaa gttctgcaaa aggcaaaaac
    11581 agctcctttc gcaggcgatc cgccaaggat tccccttgca acaattcgaa aaccagaaaa
    11641 agcgagccat ccgacgtcaa ccccttgtct aacagggcac acacaaaagg gctttgaatt
    11701 cgtgataccg cttccgcctc tctttcgaaa cgtcgctgaa tatccttgcg acgcgccgat
    11761 cgctcgagca gcaccttgat ggccaccggc ccttgatccc cctgccccgc ccatacctcc
    11821 cccataccgc cacggccaat caacgaggtc acccgatagc gcgcatccaa ccgatctcct
    11881 tccgaaagca ccacgtcgac cgaaacgatg gcacaagacc gccttttgtg gccagttctc
    11941 gatgagaagc ggatcggcag aatccacccg ataccatctt acccacaggt gtattacact
    12001 gcgccccgat ggcaaaatcg caacgagacg tcgaagccta cctccaggcc ctcggtaagt
    12061 cgtgggacaa gctcgaggat gggacctata tcattcatac gagcaacgaa atcccgatcg
    12121 ctctgcggat tacgacatcg ctgctcgtgg caagggtcct cataggagcc attccaaaca
    12181 ccaacgccca gcaccaagtg caattgtttc gacgcttgct cgaactcaat gcttcggagt
    12241 tgatgtatgc ttcctatggg ctcgaagacg gaaacatggt gttgtctgcc gccttggaac
    12301 tcgagaacct cgacatgaac gaactcgagg cggtccttgg agacatcgat ctcgcgcttg
    12361 cccgccacgt aagcgaactc tgggaactct gcaaggaagc ctgatcaacg ggcggtcatc
    12421 gaaccacgag gaaatcgaag catgggaatc ttcgcccgcc tagcacaact catcaagtcc
    12481 aacatcaacg atctgatcag ccgatccgaa gatccggaaa agatgctcaa tcagatcgtc
    12541 atcgatatga acaaccagtt ggcggaagcg accaaacaag tagcggttgc catcgccgac
    12601 gaaaaacgac tctccaaaca cgtcgagcaa gaagccgcca acgccgcgga atggaaacgt
    12661 cgtgcggaga tggctatccg cgctggaaac gaggaactcg ctcgggaagc gctggctcga
    12721 cgcaaagagc atgagcagct tcgcgtgggg ttcgaacagc agtggcaaaa acaaaaggcc
    12781 gcggtcgatc agctcaaaag agcgcttcgc atgctcaatt cgaagatcga ggaagccaag
    12841 cgaaagaaaa atcttctcat cgcgcaaaag cagcgcgctg aagcgcaaaa agccatccat
    12901 caagccatgg gcggcgtgcg ggacaacagc gctttcgaaa ccttcgaccg catggcggat
    12961 agaatcaatc gaatggaggc cgaagccgaa gcttccgccg aattggcgga agaatattcg
    13021 ggcgacgtgc tcaagtccaa gttcgacgag ctggaacaga cggcgggggc ggatatggaa
    13081 ctgctcgaac tcaaacgaga aatgggattg ataccgccgg aaccacagcc cgcccaagtg
    13141 cgcgtgcaac ccgtggtaga agccccacca gccacggcaa gcctcacgag cgaacagcaa
    13201 gaacaagatg aattggccgc cgccctcgcc gaactcgaag ccgaggagct acaagcccaa
    13261 cgcaaaatgc aacgctgacg ttccttcaac caagtaccat aaccttcctt cgagcatact
    13321 cgttcaaagg ttccccatgt cgctaccgcg atggctttgc ccaaccctgc tatccctggc
    13381 cgtcatgatc tccgcgtcgg cccgtgcgga tgtcccccta tttctcgcca aaccaggcga
    13441 aatcaaagtc gatggcgtcc tgcgggattg gggcggcacg ccgtttttcc gcctcgattc
    13501 acgagtccaa ggctcctccc ccagcaacgt acgcgccgca ctggcctacg acgccacctt
    13561 cctgtacgtc gcggcccagg tcgtcgaccc ttctttcgtg cgcaccaatc gcttcctcga
    13621 caccgaagac catgccgaat tgttgctctc ctttcccaac gcacaaggcc aatacaaccg
    13681 cgttttcgaa gtgaaactgt tcgcgggcaa gccaggagaa acagaaggac aagtcaaagc
    13741 cgtcggcctc ggccccgttc gcggagccga aatcgtcgaa gccccctccc aagggggcta
    13801 taccttcgaa gccaaaattc catggtccgt ctttcccccc gccgcccaga ctcgcgtcaa
    13861 actccgaggc gccttgcgct acctcgatgg cgacggacgc ggcacctcgg ccatcatcgc
    13921 ttccgctccc tcgaatcgtg cagccgatct gccgctgctg ctcagcgctc ccgaacaagc
    13981 tctcgtccgc ggcctcatca acgataggaa attcggctcc cccaccgccg atatcatcgc
    14041 caacgtcgcc ggtgactcca tgacggaacg cgtacttctg ttccacaata ccttggtcgt
    14101 ctacggcccc acgttccgaa aaggaaccga gtatttctgg tccgacatca ccgaccccaa
    14161 cgccggacaa ctacccctat tcgacgttcg cgacctgact ggcgacggca aatccgaaat
    14221 catcgtcgtc aaacgtgttt ctcgcggcac cggttggcga caaatgctcc aggtcctgtc
    14281 gtttcgcggc gactcgctcc aacccatctt cgaacatgaa accggcctgg gttcctccca
    14341 aggcaccatc gaaaacaaag tctccctcgt accctccggt cagggacatt ccatcgtcat
    14401 ctccctcggc tccgatagcg gttacgatgc ctccacgtat cacgagccgc aagaaaccga
    14461 acgagaaccg ctgctcttgc cctggggtac cgtacaatcc cgaacgtacg cctgggatgg
    14521 aaagcaattc gtcaaacgct cggagcaaac caaagccgcg gattcagctc ccgcgcgccc
    14581 cgcggctccg ccccccccac gtcctccctc cgccgacgaa ttgctcgatc aggtctaccg
    14641 gctttacaaa aaagatcata atattccaga ggttgcaaaa cctaccttcg atttcgtcac
    14701 caatgtggcg gcttcccaag aagtcgaacg catcatctgc cacggccgcg atctggtggt
    14761 ttttggcaaa ggatttcgcg aaggacgagg ctacgtatcg ctgagcatgc cgcaattcgt
    14821 ctcagacaag gatattcacg atgtttccgc cttcgacgtg accggcgacg ggctcgcgga
    14881 tatcatcgtt cgtggttccc aaagaaccaa agcccccgag gagttgggac agggcgaatt
    14941 ggttcgagaa gttctgttcg tctaccgcgt ggatgaatcg tccatctccc gagtatttgc
    15001 cgccgagact gccgtcacgc tcggttctcg acgcatctac agtttgctgg cgttcgttcc
    15061 ttcggcgaat gggttggata tcgagattcg gccgggacga gccatcggat ggacgcgtgc
    15121 gacatggcca tatcgccaag acaccgaggc tgtcagcggg ctcgagccga ttgttttgcc
    15181 atggacagaa caaccatttc gttttcgttt tgccgggcag acgttcagcc gataatccgc
    15241 atgagattta atcgcaggca tcattcttcc tcgccaggag cgaaaagacg tgccatgtca
    15301 atggatgcgc catggctcga ttatccgcgc tgcgttcgtc tgaccccagg gaagacgccc
    15361 ttgattcgat cgaggtgccg gcggcggtcg tttgtgcgcg ctgcggaaaa cccgactgcc
    15421 ccggctgcga ctcgttggac gaaaccacgc tgccgtcggg agtcatttcg atcgtgccgt
    15481 gggaacgccc tggcacatct gcctggtcga gattgtggtt gaccgcaagg gcggcaaccc
    15541 gaagcgccgc ttcgtttttc caaagtctac cgccgggttc ccacacttcg gctttctcgt
    15601 tttccctgat cgccgagttc tatgccgtcg tatcatgggc cgttttcatc agcctgctcg
    15661 ttgcattgcc tttgcacgat atcgcatggc tcatgctgaa aaacccggcg ggacgcgcca
    15721 tgctcgcgcg tctgtgggtc gtgggcaccg tgggattcac ggtcgttctc gtggtcggtc
    15781 atgccatcta cggtgtagcc ctcgacaaag gagcgcgtcg gacgggcgcc cgctcgcgct
    15841 tttcccaagc gctgcgcttt ggattgtatg cgtcaggctg ggatttgttg acgtcacccc
    15901 taggactgct cgtatcgctc atcgcggaag gtccacgggc cactatccac ttgcttacgg
    15961 catcgatggg acttcccaga cgcgctgcta ccgcgttcct tcgcggtatt tatcatctgg
    16021 aggggcctcc gctggcgcaa gcacgtaact acggaactac agtcgccatc atcctgagca
    16081 tcaccggcgc catcgtcgtt ctggtatcgc tcgtctttgc cgccttggga ttttagtcca
    16141 ttacgcccaa tccttgctcc cagtcccgga cctaacggtc acggcactcg ctcacggcca
    16201 cgctcacggc cacggtcacg gtcacggtca cggcgcgcgg caaacgcaaa acggtccaca
    16261 aggaccaaaa agacgctcaa cacatcgaaa aataccaacg ctcccatggc atcctggctg
    16321 agcaaccatg acgcgcctcg cccagaatga atccttcgcc gagcttctgg aaaaggtttt
    16381 gcagcccgcg aatagcacca aacgcgccca aacaactgta tttattgatt ttttagcacc
    16441 gatcaccatg gggacaacgg ccagatcaaa gttcagggga ttgcctgcgc gtatcccttt
    16501 cccaatcagg aaaataggag ggggctgtca gccctgtggc atgccccatc cacgacgccc
    16561 aacggcgatc acggtggaaa catccgcaaa gaaatatcgg ttcgtcgtga tcgggcgtga
    16621 cgccggcgtg ctcgtttccg ctcagcttcc gagacagcca agcagcaact ccaccttggc
    16681 tccttcatcg gcttgtactt tggcgcacga ggcgggacac aggcggactt tggtgggatt
    16741 ggccggattg tcgtaataaa atccatcccc cacgcattga gcctcgttgt cgaccagagg
    16801 aaatacctct accgctccac cattgctggg cgtgtattgc acggtcacac gggaccaatc
    16861 gatcaccccc gcatcggagg tggcgggcgg tgtgagcagt tcacacgcta cggctttcac
    16921 gatgctcgtg gcgatattgt tgaaaaccgc cgagtaattc gtgtcgcaaa caggatgacg
    16981 caacccaccc gtcaagatgg aaatctcctg atattgcggg cccgtgttca cagccgtaga
    17041 gcatttggaa ttcgttgtgg gctcggtggg agccaggggc gcatgcacac caatgattga
    17101 atgaaacacg tagtttcgct ccgcgctcgt cccgaagtaa ccagccccat tgccggtcaa
    17161 caaaaaatta tcgaaattcg tggcggacac agaggattga tcgtcggtga cttcaatgaa
    17221 ggctttgaag gcggacggtc gcaagttggc cttgatgggc gtccagtagg tgtcattcat
    17281 gaatagcgaa aaggaattcg tgctcgcaat cgtccggttg acctgaaaga aatgcggagg
    17341 attgtcttca ccgcaggcag ccgcaccaag aggcgggtcc acacatagcc gataggtcgc
    17401 ggaaatgccg ctggttccat tggcgaataa gatgactcga taatccaatc ccgcgttttg
    17461 aatgatattg gcgaagttgc cgttgacgtt ggcttttacc tggctgattt cattacccat
    17521 cgagccggat tgatcgatga tccaaatgat atccacgggc ggtttgaccg cttcggccgt
    17581 caccgcggca caagcatcgg gctccgtgac atccgggggt tgatagtcga aagaaacgtc
    17641 tttggcgcca ccatcggtgg aacctgctga agctccgctt cctgcgcttg ctgcgcttcc
    17701 cgcgtttccc gccgcattgc cgctgccccc ggtacttccc gcgcttccag cggtcggtcc
    17761 actgccaccg ctttggctac ctcccgaacc accggcggcc gacgatcccc cagcaccagc
    17821 gtggggcgtg ctttcatccg cttcttcttc gctgctgcaa gaagaggcaa ccccaaccaa
    17881 agccgccagg accaacactg gcgtaaaaat tctaccgcga agccataatc gacgcatctt
    17941 gccatgatga cacgttatcg cggtttagaa agggctttta tgaggagtgc ggtgggtcct
    18001 cgcaccatgg tcctcatgcc accaaccacg atgacaccac gaagaatcga gcggcgcggg
    18061 aaagaaaatt tccttttaat tacaaatagt tactagctgt tcaaacaggt cgcatcctgc
    18121 gttcgggcca ccacccccac gccgtcgacc ctcgatgact cgcctccaac gcaagcccgc
    18181 ccacgccatc acgcccaggg atccaaagcc gtcggctttc gaatctgcgc cagaagcatt
    18241 tgctccgtca aaaaccactg ccccgtggac ggtttgcgcc ggagtttgac gggccggggg
    18301 ctatcggcgc cacccgagcg caggtaaagc tgggcatggc cctcggtcgc aaaactcgtg
    18361 ggtaccgtgg aaacggtcac ggtgtggggg ctttggggct ggtagccgtt ttcgggtttc
    18421 gccccctcga agtaagacag agggacatat tcgttgccac gcagacggtc cgcgatgaat
    18481 tgcaggtcga acttggaaag aggttgagga cctttcaaaa agttgatcat ctgaatcgcc
    18541 tcttcccgat tcgtgggcca gaggcaaagg gcggggatga ccagagcggc agcgtaatac
    18601 ggctctttca aggaagcctc cggcaacgcc tgaagcatcg aaagggagat gggcaagctt
    18661 ggaaaaacga cttgccacgt ttgattggct gaaaccacca tcacgcacct gctcgaaaga
    18721 acgaagacct tgctctcggc cgccatgcac aaggccctag agcgcttgcg acaactggct
    18781 ccgcgctttc ggttcttagt acaagacagg acaaatgggt atccattgcg cccaagttcg
    18841 agccttgttc atgcctcccc aaaccgtgat tcaaccattt cccatcaccg ccccccacgt
    18901 cacccaagcg aacggcccct acctatctcg aacgtctgcc cttgcgcggg aatacacaaa
    18961 aattcgcgtg cgaacccacg gcgttcccac tctttttcca gccgcaaagg cggttcatcc
    19021 agaggttcat cactgagctt gaaagtcccc caatgcatcg ccacgaaata ccttgccccc
    19081 agttcttcga acgcgcgaac tgcttgctcg ggactgacgt gctgcttttg catgaaccag
    19141 ggcgggtcgt aagctccaat gggcaataag gcggcatcga tgtcggaaaa tcgttttcca
    19201 atccccgaaa acccatcgaa ccaagcgcta tcccccgcgt gatagatcgt cgcagacgaa
    19261 cctcgcacga cgaaccctcc ccatagccca cggttggcat cccaaagccc acgacgactc
    19321 caatgctgcg ccggcacgaa agtgattcgc accggcccaa ggtcggtcgc ctcccaccaa
    19381 tccaattccg tcgtttcgac tgcagtaccg aatagcgcgc gatgtcccgg accggtgatg
    19441 atcggcgaac cgaccgcgcg aagcgatggc aagtcgaggt gatcgcgatg gttgtgcgtc
    19501 acgagctgtg cggcaatccc gggaagttca tgcgcggcca gaggcgctgg cacgttgcgc
    19561 gcaatcccag gtccaatcga tcgcgaaaat accggatcga tgagcagcga tagaccatcg
    19621 agctgcacca accagctcgc atgaccaagc caggtcagcc tcgctcccac gctgggcggt
    19681 gtggtaattc tttcgagatc caccgccatc acaggaaccg aagcgcgaga agcggaacgt
    19741 cgtcgcctgc ccaggatgcg atcgagcacc gcccacttga aaacggaaga aaaggtctgg
    19801 ggcacgctcc catcgagatt ggcaaatcgt ccggaaacaa ggcgcatcga ggattccgtc
    19861 gaggcaaaga aaatggctat ggcgaccgcg ccggcgacgc tgggcgcaag gcgatgaagc
    19921 gatcataacc cacgagtcca aggatcgtgg cgatgcccag tcccacgaag atcagccata
    19981 gggtgcgagg ttgatggcat ccatccacga agtgttcata aagcttcgcg ccgaaataac
    20041 cacccacgct cgatccgatg acgccgtaca agaaggagta gcccatatag gtcgcttttt
    20101 tgtcttcggg ggcgatgagc cccacgtagg agatgaactt gggatgcgcg gtcatctcgc
    20161 caatcgaaaa cacgatgatg ccggccatga agatccacgg gctggtggaa atcgccagca
    20221 gaatgaagcc cgccgtgccg atggcaatac cgccgatcat cgtggggaga gcccgccagt
    20281 ttttgacgat gctcgagatg atgagttgca agacaatgat ggtacccgcg ttgatgaccg
    20341 tcacatgctc ggaatcgaac ttgaatttcc catcgaaacc gagagcctga agaacgccat
    20401 tgatggtggc gtcgaacggg gacatatcga catgggcctt gagataccaa aggacggtat
    20461 cgaacatctg gaagtaaagg acccagaagc ccgaatagat cgcgatcatg gtcacgaagc
    20521 gccagtcttt caggaccagg acagcgccgc cgagcacttg cgcgagcgtt ttggtacttt
    20581 cgggtttggg cggttcccga taaagaaaaa ccgtgggaat cagcatgata cccgtgccca
    20641 gagccgaggc ggtgaagatg gcggaccacg gataggcatg ggtgcgcagg tagttgacga
    20701 gcacgagggg aaaaagaaag gcgccgaggt tgatggtcca ataaaaaatg ccgaaaccaa
    20761 ggccggaatt gctctccgtc gtggaccggg cgatcgtgcc ggaaatgatg ggtttgaaaa
    20821 tccccgcgcc cgtggccatg acgatcaacg aaaggaagac cgccacgtaa ctcgtcatct
    20881 gaccggccag gaagtatccc gtggacaaca agcagaaagc cacgagcaag ctgcgacgat
    20941 aaccgaagcg atcggcgacg gcgccggcca cgatcggcag aaagtacaac aagggacgga
    21001 tggtgctgtt gatcgcgccc gcggcacctt tggaaaaccc gagaccaccg tcggccacct
    21061 ggtccaccat atacacgctg agcacggaca tcaggccgta gtaggagcct cgttcgaaaa
    21121 actccatcaa caccacgacc caaaacacac ggggaaagga gcggaatccc gactccttcg
    21181 aggaactcgt tgccttgtcg ttcatctgga ttcttcaggc ggatcgtcga acatctgcgg
    21241 cgggcagggt tgcgcaagtt cttgcaatgc ctcgatttcg tcgcgcaaaa aatggacttg
    21301 cgcatcgagg ctacgggcct ggggacaagc ttccccccct tgctccaccg tcgataaata
    21361 ccgctgctct acctgttttt tggtttcgat cgcacgaacc accgctacgc tcacgcccgc
    21421 ttcacataca tccgtctggg caccgaccgc atgttcggcc tcatccaccg cgcgctgggc
    21481 ctcttgcaga cgcgcttgtt gcgcgggccc aggaggatcg aaattcgacg ccaccaccca
    21541 aagacgcgcg agggttcttc ttccaaacac gtttttctct tcgcccaagg cgagaaaaag
    21601 cgggtccgtg aacttgctgt acaccaccgt cggcggttgc tcctcggtgg cggtgggagt
    21661 cgaaggcgtg gctggcgatg tcgtcgacgc ggaaggacca caggccacgg ccaacgatgc
    21721 actcacgatc cacgaaaagt acgagacacg tttcatgcgt ggcgacatca ttctgcctga
    21781 gttttgaagt cttttccgat ccatcccttg gccaatacga gaaatacggt ggagctgacg
    21841 gcgatgattc cgtagatgag ccacatcgtc tcgctttgca ccggaccact atcgggaacg
    21901 tacttggaca agaaataccc tgagtacagg ggcacgatca ttttggtgag gaaccatgga
    21961 aattgagcca cgcccatata cgcagcggtg cggccctccg gcgcaatctc cgcggcgtat
    22021 tgcaaaaatc gaggctgcca catcgcctca ccgatcgtca tgatcaccag atagccgagc
    22081 aacgtccaga aagtgggacc gagcacgagc aaaaacgtgg gacccgccat cacgaacgtt
    22141 cccacgatca tcatgttgta tacctttcgc cgttgggtca gcgcggtgac gatgggtacg
    22201 agaatgaaga tgagcagcgg attgaagttg accgccactt cgaaattttc cccgagccac
    22261 aagcgcgctg tttccgacca atttcccttg gccgaggatt ctttggcagc cgcttgaaag
    22321 tccagagttc gaatggtgcg cttggcttct tccgcttgtt ccgctgcttt tttgtctcct
    22381 tccacaagct tgacggcagc catcgaacga atttgctttt tggcttcaca ggcctgcttg
    22441 gagccttcaa atcgcaagac aacatctttg agcgcttcta cggtgcgcgc gccttcaagc
    22501 gcaggcaaag cctcggacga cgaagcaagc gagtcacaag cttcgactcc cacgggcgga
    22561 gcaaacgctc gctcgacgta caacggcaag gtgagccagt tgtgggcgaa gagggtttgc
    22621 acaggaatga gcgcgaagat gaagaaggtg aacttcgggt ctgccaaggg atgatgacgg
    22681 agccaatgcc aggccatggc gaagaagcca cgggcccggg cttcagctaa agcagcgttt
    22741 tctttctctt ttttctccgc ctcttgctcc tttttttcgt cggacttctg cgcttctttc
    22801 tcggctttcg cctccgcggt cgttcggtcc actacctttt tggtaagcaa cacgatggta
    22861 agcagcagcg ccacgacgga agcggcggtg aatacccaga aggcgccact gatgccaaat
    22921 gcttttcgga taggcgaaaa gaaggtgggc acccaaccgc cgaggttcat gagggcatag
    22981 agcatggcaa agcccatggc ggcagttttg ggcgtggtga attggcgcac gccggcataa
    23041 gccgcgggct gatacatgcc atagcccagc acgatgagca ggatgcccac catggcgatg
    23101 atgtgtacgg gggagcccaa tccggtgctt cccatgccca cggacggcgc cccggaaagg
    23161 gcgatacgtc caccaatcat gaggaaaaac gcggcgatga gagcgatgcg aaccccccat
    23221 cgatccgcaa gcccgccgaa caaaaacatc gaaaacgtaa tgccactggt gagcaccccc
    23281 accatgatgc ccgccatcga gtcgctcaat cccacgtact cgttgaagta catggccagg
    23341 tacatgagca tcccgaagta caccatcccc tcgagaaaat aggccgcatt gatgccccac
    23401 agcgcgcgag gcgcgtgtac gagatcgatg aaaggctgta cgatctcttg aaaaggattg
    23461 ggtttggtct cttcgctgtc ctgatccggt ttgttgtcct gctcgtcggt gctcaaggca
    23521 gcctcctatg cgggatatgg gtgggtccca cggcttgaaa atccgcttcc cacggtccgt
    23581 gcaagccacc gcggccgaag cggcgcgcat catgccagaa gagctgtcgt aacccaacgc
    23641 cagacaaggg ggaacgacca tcgaaaataa agtctccacg agcctctatc ggtgacccgg
    23701 gccccccaaa gccgtcgaac aacgctttct tttccttggt ctttgagcaa ttctcacgcc
    23761 aaggccgttg acctgcgctg cccatcgccg ccatggtccg gctgttcatc ctcgaagtcc
    23821 aaggagcgct cgacatggcc gattccttcc gagaacaaat cgccgccggt atccccaaca
    23881 cattgcccga attccccccc
//
LOCUS       DAOSOR010000014        23881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_2135_length_23881_cov_5.029799, whole genome shotgun sequence.
ACCESSION   DAOSOR010000014 DAOSOR010000000
VERSION     DAOSOR010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 23881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23881
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2135_length_23881_cov_5.029799"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="PK710_01445"
     CDS             <1..1242
                     /locus_tag="PK710_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOT08396.1"
                     /translation="CWAAEPVAATVSSSVFWLPWLTRLGPRFSVVGVAANGLAVPVGE
                     LISLPACLGHLLLAPFPDAERGVALLGSGSLLFVRWIARNAASLDVLAFDTPRPTDWH
                     LVMVGLLGACLLLYPRRRFFWAWLGGLLWVVFESWAVFCHQPKGRLRITVLDVGQGDS
                     VMVDFPDGRGMLVDGGGVVGSPMDPGLRVVAPVLRARRRNVLSFAVLSHPHPDHVIGL
                     ASALPRVSVGEFWDSGQGERQGAGPVYQGLLDGLRARGVMVRRPPELCGKAFYFGQAR
                     LRVLGPCPDIVPGRSANNSSIVLHVQYGRRAALLVGDAEHEQEEDLMKLGPSVLHADL
                     LKVGHHGSKTSTSAAFVDAVSPRDAIITSGVRNRYGHPAPNTLKLLKEKGVRVFRSDV
                     HGAVRWETDGESLWVKTAISP"
     gene            1454..1768
                     /locus_tag="PK710_01450"
     CDS             1454..1768
                     /locus_tag="PK710_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08397.1"
                     /translation="MRTIRDEVERPNEWLRRLSEDPLQYRQLLEDAGSVGRAAYRLAR
                     ARCRTRPIAMNIPTRLELHAAAQELQSRVEGMPSLPSIEELVWDCESAGLVVIVPLGR
                     AA"
     gene            complement(1787..3802)
                     /locus_tag="PK710_01455"
     CDS             complement(1787..3802)
                     /locus_tag="PK710_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOT08398.1"
                     /translation="MTPLDPELRRLLIEELQKHLDALSRHDLDSKTQRLVLHSVKGAA
                     GLVGETDLSDAFSRLERLDNPAHSTRLARTILQQALANLNANRQAYRDPWPNPPDDLW
                     IHGNWKCKDKLYYSDMMDRLREWDLSLSSDPHDGLKDALRHLHTLKGAAAAVGDEVMA
                     WFCHGLESHLSPCQNPEQAQSLVDQMERWRPVLAGLLTDPTATLDLLRANSPGSVPHT
                     PLPSTVAFDPSAHPWLQVPTQTLDLVLERLRRIHRSAREMEGQTDFARNTARQLRDAK
                     QRLSESQRLIGPPRPWGAPLAALHHIENASEILTLAACQLDSLASTTRRGTRSILQST
                     TSSHNELRAVRQATVAVVFDRVAEAVQTIAERSFKKVQVEQVGTNTPIDRHVANSLVD
                     PLLQLARNAVVHGIESPDVRAAVGKPPFGRIRLSAEIRGAHLILRVADDGAGIDLQAV
                     RAKATSAGRLLADASPPPSDEALLNLLFLPGMSTHPNVDLLAGRGIGLDIALHTIRRL
                     GGTVRLRHRKGHGLTAIVDIPVVERGHALVLWVRSAHDRFALTARHLLQTHLANPHHP
                     APSLASLMDPQHLGLPGKFVIEVGKSIDDAIRLTVDEIEGFEYAPVRPVPPLVALAGP
                     YVGVVVHTDGHPSLLVDALLVAERTWKTPYQRYSTFPPVERKERKIK"
     gene            complement(3799..4569)
                     /locus_tag="PK710_01460"
     CDS             complement(3799..4569)
                     /locus_tag="PK710_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT08399.1"
                     /translation="MTKGVRRGWFRRARQTSKPTDQTEVARLWTAHLRAHQSANHAVE
                     LTTQLLASTKRQQVLLDGALDLIRQAANRSPEIQDPIDQVAHNLDRLRLIALNVGLEG
                     ARLGDPAGHTLMNMADEVRSLTERANEAVQELRTALDDTLSLWNQAVHRTEETRHAHE
                     ALETQFGSLQTAADNTLQDIQPIGTHTRSLLDMDPHTAVALARVTEHAKELIQALGSL
                     GDHARQDIIRSALGPTLHPLLRALLQIANPSKIKPIRE"
     gene            complement(4566..4964)
                     /locus_tag="PK710_01465"
     CDS             complement(4566..4964)
                     /locus_tag="PK710_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08400.1"
                     /translation="MPPCAGMVLDAGESWWFVSAQEAQWIAPAEPVLPVPGMVPPAIG
                     LVLTEEQVATALQVGSAPASDMIMCMTQGGPVALFGARVVATGVFELTEGQVKFQNKS
                     ARLLDVRQLTFDIEAALWRAARLGEQERSE"
     gene            5335..6054
                     /locus_tag="PK710_01470"
     CDS             5335..6054
                     /locus_tag="PK710_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08401.1"
                     /translation="MADKRVSTKSKKSTGAKSKKAVRSERRFMPERSNASIATMAGTI
                     VGGLGVGAGLYGQWMTNPTMAVAPWVLAAGSAVLAAVLLWGDLEGTVIRVGDAGIVWE
                     RDGKVNRRVAWCDMREVEIRDAMVRVQLEGEVMTFSVASQPLAAAWVAKEANARIPKR
                     WKASKDVIQSLPKTSSDDGEGLVVEPLQVTGRSCRASQRTITFERDARYCVRCGEVYH
                     KDDLPDRCLTCDADLRDAQEA"
     gene            6486..6686
                     /locus_tag="PK710_01475"
     CDS             6486..6686
                     /locus_tag="PK710_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08402.1"
                     /translation="MLTVILAADGTSTFFDCGKKGCVVLWAQGFVGAGACHGRGVDEA
                     QGSVCGKHWGMFMGCGVGLRGG"
     gene            6943..8700
                     /locus_tag="PK710_01480"
     CDS             6943..8700
                     /locus_tag="PK710_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08403.1"
                     /translation="MLRNVRKITPLIRSLVLGVAAVSTVSVSSAVMMGCEDDKQPEYH
                     VKRLNDPALRPQAVKRLVQFFEDAMTRADKDRTKPNVKELLDKIVPPLTQTYLEGDTI
                     DDRTKGEIIKLLADSQDERAKPAWIKALKDYKPNVTEPDVRACAKVIAETGVKDPEAM
                     DALINVFVKFEAGSPKGALIYVDFKKALVDISSPSWKSQLLERLNRPMEKLEQSDRTN
                     EDKITAYRNEQFWQVTSAEILGEIKATDAIKPLFKTVIDPNKADVAATAIMAMVKMGK
                     DSMNTLIDALLGKDAELTEFAKAKIKAEKEALVTPVRSAALVIGTIGRADGVQPMLMA
                     LDKADKSDAITQAILARELSKCPVTPESLKAFIKVFNDMPVSAFIPPGLTAVTVLAES
                     IAQFYDPSVIDTLIARVDNFKGDKQDKDLLRDSVLPTMIKLMRKDQIDLVEKAIGRWA
                     PGKDDAKLEKEALGKAKAVLSACDDKLECYLAKLEEAAVQEKNEQFQGIKASYMLGIL
                     GNDKTRMEIVRRFPSIKNAAIKYTAGQALDFLAPKGDQAAADEIDKQLQTNIAKGDQN
                     LIAGDAPLRQIRIRLLARL"
     gene            8704..10632
                     /locus_tag="PK710_01485"
     CDS             8704..10632
                     /locus_tag="PK710_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT08404.1"
                     /translation="MIQVTVHSGLQAGQWLTFDEDVVTVGRASDNHVILADSHVSGHH
                     LRIVRGPHRTLIEDLHSTRGTTVIMGDERKRLDEMPNRCVEVDTGCRIEVGTGAELTL
                     IEVRLLESDEARVVSMRRIEEIGVSGENLERDLQILPQLYAAQKRLDAASDLDEVLEA
                     TCDGVLQLLPRATHVTVTLRDEEDSSQEPVYVPVVTRERGTDGSGAASRGAIPIARSV
                     FRKVLSERAAVLAADAPREVAQAESIVGASIRSTAAVPLWRGETILGVIQADNRQAPA
                     MLSALDLEILALFASNASLAVSNARLIRRLQIAQEQLRKENDFLRTREETRTRAQPIL
                     GESEAMKAVLAQLDKVVDTRVTVLVEGETGTGKELLASAVHYRSKRRHKLFVAQNCAA
                     LPENLLESELFGHKRGAFTGAVDDKKGLFDIADGGTLFLDEVAEISLSLQSKLLRVLQ
                     EGEVRPVGASRTHWVDVRIVAATNRNLEQEVREGRFREDLYYRLKVFPLRSPALRERR
                     EDIPLLAGAFMKRYASELSKTVGGFSQQAMELLVAYDWPGNVRELQNEIQRLVIQLDE
                     GAFVVPELLSERIRSVEGVVGKAGVGKGTLREMMDRVEKYLLIESLREHGNNKTNAAK
                     TLGITREGLHKKLRAHGL"
     gene            complement(10639..15111)
                     /locus_tag="PK710_01490"
     CDS             complement(10639..15111)
                     /locus_tag="PK710_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT08405.1"
                     /translation="MSLQFPARYEQVAVLGQGGGGQVWAVRDRLTRETVALKALAENA
                     SQKEVQALVREAVTLSGLEGFGVPKVLRFGRLPGTLRPYLVRELVPGHSLLSMMEGPK
                     PDPHTCLNALLHAANQLTGLHRASLLHGDVKPANIIVSPQGATLVDLGLAAPWREEGA
                     RPEGLTPRYAAPELFAGMPITVRSEIFALGATLRDVLEACGHQLDPSILSNLHDIALR
                     ATHTDPSQRHPSTDEIASAIRLAAHLPDVSANHAVDLVWPIVGVDAAANLLLSRIATM
                     VTGSVIALVGKIGSGRSTLLRRLAWTLGIEGRDVAWIQGEQSVPWTDELEMELGAYPT
                     LEGVVVLADGAESLTETDSQRLAQAREKGAKIVLVAHPHVATSIGGPVETFVMPALDH
                     EAAAELVRRLIPSLSDSLVQHTIRRAEGRPGKLRAIVRRIGSRPVLSISEMDEMWQET
                     ESTVSHPLLSSDIAEVDRLLNQGRLEEAAEKLQQVSNTDPLEYALCKARLELGQGEAA
                     QALATLQSIDESSIPSQGLARRRWMLNVARCKLRLGCHEESMSYARQAIDLEATDAER
                     IAVTLEARACIGLLHSYGGDHAGAEQELMDAAAAARQLGDKRVESIVVGSLAVALQRG
                     DKLAEARRTFEEALECAEHAGDAGAVANTRLNLAVLANIEGDLADAIEHLEAAIDMGR
                     RASRGATTRQALLNLAHLDIYLGRYARATSSLASLAEQREKLAPNHRAQLAGMQAELA
                     ARTSELDRAVEFYASCASQYEALGRSVDAAEAILEGVLVSVRSDSRDLMSLSRDIEQA
                     MSLLTDNTAHRALLHLARGAVAAAQNDEDRARNSFNAALLAAREAGQKEWIWRTLEAR
                     SRLLVEMGQDLAARKDAEEALAVLEEIATRLPRDLREVFWDDPRRRAVRTNLLGMQLP
                     RSNAIVANSLWPLGSRASGTGISSTMIALPVEDRLTKLLEINRELAREHKLEPLLERV
                     TDHAISLVHAERGFVILAQNGVLVPHISRNRQGDASHAKFSSNIAEKVIATGEPVITL
                     SAREDERMADYLSVHQLNVQSVACVPIRAPDNRTAGALYVETRHRPGALFHTELPTLM
                     AFADQAAVAIANAHLIAENQRRAEELAKTNAELEKAHARLQEVLGHRTAQLESTRRDL
                     DATRAVLRGHFGYQGLVGTSEKMRRVYALIDRVKDMDVPVLITGESGTGKEMVARAIH
                     RGGPRGKKPFTGINCGAIPEHLLESELFGHTKGAFTGADRDRKGLFRETEGGTILLDE
                     IGEMPHKMQAGLLRVLQEHLVRPVGGAREERVSVRVIAATNRNLERMVAEGTFREDLY
                     YRLRVVDVQLPSLRERAEDIPMLIDHFLNIFAARYRRERKTISRDALRLLCACPWPGN
                     VRQLENSLLNAWVMSDRAQLEIDDFEMAGVQRTSDSRSSELPPSFISEVHPVDTGARK
                     HLGTSETLSAHEASEKERILAALASANWNRVKAAELCQIPRRTFYRRLKKFGIQ"
     gene            complement(15119..15988)
                     /locus_tag="PK710_01495"
     CDS             complement(15119..15988)
                     /locus_tag="PK710_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08406.1"
                     /translation="MKNCVVAPLIAFSFATLATAVDAQTDPASPSQSPSESLAATDGT
                     AAFPQEKHNPKQSPAAADSSRNEGDAEDQRFIGLRGRIAYIPSWMFDLFGADGGKGVV
                     SPSVGPEYVTRKHGFETDIWLVFISYGMGDVPFKAKSDPDTSYEIVRSHIKTLTIGAD
                     FLWSKPLGDPRFTLTYGAGAGIGVVFGDLIRNQAYPPDGQPGDPTTYVKCPGPGRAHG
                     DYCDASNDHYNDYQEPNWLHGGAKPILFPWITLPQVGVRWNASRDFVLRLDTGLSFPG
                     PWFFGLSGQVGLR"
     gene            complement(16205..16501)
                     /locus_tag="PK710_01500"
     CDS             complement(16205..16501)
                     /locus_tag="PK710_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08407.1"
                     /translation="MSLKKKALHQGMKLMSDPRVMKLMQDERLMKAMMAAMSVPGRLT
                     TAAHEHADRVAKIMSLATEQEVRDLRRTVRSLEDQLADLKRELEHARSRRDDSN"
     gene            17088..17648
                     /locus_tag="PK710_01505"
     CDS             17088..17648
                     /locus_tag="PK710_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08408.1"
                     /translation="MNPSLSIFALVLFVMTVELCQPMNQRDYQAQVIDAADRLTDELL
                     GYFGELLQSVVRDVTVGIANTAPSTASKNRAVAEKKEQKSAPKPSRPSVSKKKQVAKK
                     PSTHRLASSGRRSAQELDKDAARVVRYLKEKKAEMRIEQINTALGTTTPQLMRPIRRL
                     LASGEIQKRGTRRSTTYFVPASSLHD"
     gene            18037..18510
                     /locus_tag="PK710_01510"
     CDS             18037..18510
                     /locus_tag="PK710_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08409.1"
                     /translation="MTRDELREKIRAAALHFTEEVSAIFGEAFASVAAEFRSGPMAAS
                     TTPKKPTPAPKPIKAAKPQNTKTTVVKPAAKTPTTAKKKPVPSKRIRRSANELGRVGE
                     EVVKLLSTAPEPMRIEEINKRLGTTTRQLMRPIQKLISDGQIKKEGERRATIYFL"
     gene            complement(18526..18993)
                     /locus_tag="PK710_01515"
     CDS             complement(18526..18993)
                     /locus_tag="PK710_01515"
                     /inference="COORDINATES: protein motif:HMM:NF024189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HOT08410.1"
                     /translation="MIPLPSCSRVLVAACLSLCLALIASCTSVTPIPPQGPHDATEPF
                     LSVPKPPDIVHVQMLPPPPDDQSVWVDGQWQWSGRHWKWQEGQWVQPQKKAHYAQPDF
                     VLTPEADYQRVISQEGAESWVLRGYRAKLQFRPGRWYSTSDRAPAGSASPLER"
     gene            complement(18990..19955)
                     /locus_tag="PK710_01520"
     CDS             complement(18990..19955)
                     /locus_tag="PK710_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOT08411.1"
                     /translation="MPTLVELPPWFLLGFALAFGLIWGSFLNVVIYRVPRGQSILRPA
                     SRCPHCETPIRPWNNIPVVSWLLLRGRAACCGAAIPIRYPLVEAAGGLLALALVQTVL
                     RPMAPDTPLWLGALTFCTYFALGLVSIAVAFIDLDHLYIPDSMSLGAIVVGLASFSLR
                     PPLTFTESLAASIAGFVVVWLVFGVLYRAIRGRTGMGLGDAKLLAVAGAWFGWKGMFF
                     ALLGGSVQGTLAAVVVLLVRGRLEEPEAVQREREELLAAVAAIEDPVERQQAEQELQK
                     DPIFEPSAGGAGARIPFGPFLALAIIEYMLVGEALLSQYMAWLAP"
     gene            complement(19965..20321)
                     /locus_tag="PK710_01525"
     CDS             complement(19965..20321)
                     /locus_tag="PK710_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08412.1"
                     /translation="MRLRSPILVCLCFAVFGCSKKDDPIDFSSQTQGEAQGAGAVPSE
                     AVSPFSSPAVSPAATSVPSVPSVNPPKPDAGPLPDGGLAMPPFPTLLPTQLPSSLPPL
                     PTLLPSAFPIPVPART"
     gene            20669..22093
                     /locus_tag="PK710_01530"
     CDS             20669..22093
                     /locus_tag="PK710_01530"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HOT08413.1"
                     /translation="MKGEKDPNEFARPLEPSPRSQAPSSRWERLARLSLLAPKVLPVA
                     LEGAKRAVGVSRSEQQERLARQRTYENAKKAGTALLKTLGEMKGLPMKLGQMFSYIDG
                     LAPPGYEDKVQRLLQQLRDKAPPLSPEAAEKMIWQEFGVGPERLFRHWEPVPFAAASI
                     GQVHRAMTRRGDEVAVKVQYPGIDKAVENDLKSLSMLEVVLAPLSRQYQSKETLREIT
                     EVFLAELDYGLEAENMDRFRQLCADLPAVVIPHVYHSLSTRRVLTAQRLGGMGYEQFR
                     ATASQAQRDAAGVTLWTFMMRNLLVHGVLYADPHPGNYRFLEDGRIGFLDFGCVKVLP
                     TAMAQGLKDYMAAVMDANWPEFDRLCHEFMGMAPGDKASWDLYRSYAIELMMPIATHS
                     TYEYTPESAKEAVQYLVRGHRKLIKEAEGGPKLPTPMHMPFDLTFVNRLQWGLASILG
                     GLRTKARFRQITEPWVRAKLESPL"
     gene            22060..>23881
                     /locus_tag="PK710_01535"
     CDS             22060..>23881
                     /locus_tag="PK710_01535"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT08414.1"
                     /translation="MGSRQAGVAFVNWIKGHWWFLLSVALGCTRAEPRSNTLSLNGSV
                     STMSVEPAVSSANSSAAEITPPQESWVEAVRLEDWALAASLLDKHVDCSAPRMRYVRA
                     KVAMGQEQWSVALTLLDGLESALPQVATDIALQRAEAHWHGGQADLAAQYFAQLKTVR
                     ALTKASQAYFRASQVKLARSAIDRAIQMAGTSSREDGIEARLHRAMLAQTMGDKATAI
                     ADLRFVYQHARDPQVSAEAWKTLRQVDPNHRLTSSDRMQRAWSHANRGYSEPTRQEIE
                     QAKAAPGGPPPRVQQLLAQAKAYYESRKDYRQAAQIYEQAAKLRGPHGAQALYLAAKA
                     WSRAEDNNRALALYEQVIHQHTASSWAERASYDAPRLYRFQRRWPLAERGYRRYLQRY
                     PRGSLSKEARYELALCLLLSGQPGQARPLFESLASGEKQAFEAASFRYLGAVAAFLEG
                     NTSLAASVWRDTISSQPLSWFSLMAQARLASMGQPTPPIALPAPSVHELPPMVVNLPP
                     TVQWLRDLGLVRDAEEAMRTQEEAFQRGYGDRGGEALCRAYGQLQTGGRLYRMGQRHA
                     PQRLVQLAPTASNRWAWECLFPYPYGPITHRWETEHGIPSG"
BASE COUNT         5122 a         5881 c         7818 g         5060 t
ORIGIN      
        1 tgctgggcgg cggagcccgt tgcagcgacg gtatcgtctt cggtgttttg gcttccttgg
       61 ttgacgcggc ttgggccgcg cttttcggtg gttggggtgg cggccaatgg tttggcggtg
      121 cctgtagggg agttgatatc tctgccggct tgtttgggtc atctgttgct ggctccattt
      181 cccgatgcgg agcgaggggt ggcgttgctc ggatccggca gcctgctttt cgttcgatgg
      241 attgcaagaa atgccgcgtc gttggacgtg ttggctttcg atacgccccg tcccaccgac
      301 tggcatctcg tcatggttgg gcttctggga gcttgtctgc tcctatatcc tcgacgacgt
      361 tttttttggg catggttggg tggattgttg tgggtggtat tcgaatcctg ggcggtattt
      421 tgtcatcaac ccaaaggaag gcttcgaatc acggtgctcg atgtgggtca gggagacagc
      481 gtgatggtgg actttccgga tggtcgcggc atgttggtgg acggaggggg ggttgtgggc
      541 agtccgatgg accctgggtt aagggtcgtg gctccggtgc ttcgggcacg acgacgcaac
      601 gttttgagtt ttgcggttct cagccaccct catccggacc acgtgatcgg gctggcctcg
      661 gcgctgcctc gggtgtcggt aggggagttt tgggattcgg gacagggaga gcggcaaggg
      721 gcggggcccg tatatcaagg gcttctcgac gggttgcggg cgcggggtgt gatggtgcga
      781 cgcccccccg aactatgcgg aaaggctttt tattttgggc aagcgaggct gcgggtgctg
      841 gggccttgtc cggatatcgt gcctgggaga agcgccaaca atagttctat cgtgcttcat
      901 gtgcagtatg gccgacgagc cgcgctgctt gtgggtgatg cggagcatga gcaagaagag
      961 gatctgatga agctggggcc gtcggtgctg cacgcggatt tgctgaaagt gggccaccat
     1021 ggcagcaaaa catcgacatc ggccgcgttt gtcgatgcgg tatctccgcg cgacgcgatc
     1081 atcacgagcg gcgttcgcaa tcgttacggc caccccgcac ctaacacatt gaaattattg
     1141 aaagaaaaag gagttcgggt atttcgttcg gatgttcatg gggcggtgcg ctgggagacg
     1201 gatggggagt cgttatgggt gaagaccgcg atatctccgt aattgtcgaa ggcaacgtat
     1261 tgggtgtcga cgggaaggaa gggtttggcg gcatgacagg gggatcaggg aaaaacgagg
     1321 gaatcttggt gagaaaaggg tagattgacg gatatttggg ggggaaaggg agcaatttcg
     1381 cgctttattt gcgaacgttc tcgaataggc atatagcttg ctgggataga gagaagcgat
     1441 aggagagcga gccatgagaa ccatccgtga cgaagtcgag cgcccgaatg aatggctacg
     1501 ccggctttct gaagatcctt tgcaatatcg ccaacttctc gaggatgcgg ggagtgttgg
     1561 gcgagctgcg tatcgtttgg cgcgagcgcg atgtcggacg cgtccgatag cgatgaacat
     1621 accgactcgc ctggagcttc acgcggcagc gcaggagctg cagagccgtg tggaaggaat
     1681 gccatccctg ccgtcgatcg aagaactcgt atgggattgc gagtctgcgg gactggttgt
     1741 gatcgtgccg ttggggcgcg cggcgtaacc ggccatccat cttccgttat tttatttttc
     1801 gttctttacg ttcgaccgga gggaacgtgg aataccgctg atacggtgtt ttccacgtgc
     1861 gttcggcgac aagcaaggca tcgactagca aggatgggtg accgtcggta tgaacgacga
     1921 cgccgacgta gggtccggcg agtgcgacga gggggggaac aggtcggaca ggggcatatt
     1981 cgaatccttc gatctcgtcg acggtcaggc ggatggcgtc atcgatagat tttccgactt
     2041 cgatcacgaa tttgccggga aggccgaggt gttgggggtc catcaacgac gcgagggagg
     2101 gcgcggggtg gtgtggattg gcgaggtgtg tttggagcag gtggcgtgcg gtcaaggcga
     2161 agcggtcgtg ggcggaacgc acccacaaaa cgagggcatg tccgcgttcc accacgggaa
     2221 tatcgacgat ggcggtgagg ccgtggcctt ttcgatggcg caatcggacg gtaccgccga
     2281 gtcgtcggat ggtgtgcagg gcaatatcga ggccgatgcc gcgtcccgcg agcaggtcaa
     2341 cgttgggatg ggtggacatg ccgggcagaa atagcaggtt gagcaaagcc tcatcggaag
     2401 gggggggtga agcatcggcg agcaagcgtc cggcgctggt ggctttggcg cgaacggcct
     2461 gcaagtcgat tcctgcccca tcatcggcca cgcgtaggat caggtgggcg ccgcggattt
     2521 ccgcggagag gcggattcga ccaaaggggg gttttcccac agccgcacgc acgtcgggcg
     2581 attcgatacc gtggacgacg gcgttgcgag cgagttgcag aaggggatcc accagggagt
     2641 tggcgacgtg tcggtcgatg ggtgtgttgg ttccgacttg ttcgacctgc acttttttga
     2701 aagagcgttc cgcgatggtt tggaccgctt cggccacacg gtcgaaaacg acggccacgg
     2761 tggcttggcg gaccgcacgc agttcattgt gggatgatgt cgtgctttgc agaatgcttc
     2821 gcgttccgcg ccgtgtcgta gaagcgaggg aatcgagctg gcaagcggcc aaggtcaaga
     2881 tttcagaagc attttcgata tgatggaggg ctgcgagggg ggcaccccaa ggccgtggcg
     2941 ggccgattaa tcgttgggat tcgctcagtc gttgtttggc atcccgcagt tgccgtgccg
     3001 tattccttgc aaaatcggtt tgcccctcca tttcccgggc agaacggtga atacgccgaa
     3061 gccgttcgag cacgagatcg agggtttggg tgggaacctg gagccacgga tgagcgctgg
     3121 ggtcaaaggc gacggtggat ggtagggggg tgtggggaac cgagccgggc gaattggcgc
     3181 gaagaaggtc aagggttgcc gtgggatcgg tcaacagacc ggcgaggacc ggccgccatc
     3241 gttccatctg gtcgaccagg ctctgggctt gttctgggtt ttggcagggt gacaaatgac
     3301 tttcgaggcc atggcaaaac caggccatga cttcgtctcc cacggcggcg gcggcccctt
     3361 tcagggtatg caagtgccgc aacgcatctt tgaggccgtc gtgcggatcg gaggagagag
     3421 aaaggtccca ctccctcaat ctatccatca tatcagaata atataattta tccttacatt
     3481 tccaattacc atgaatccat aaatcatcgg gggggttggg ccaaggatcg cgataggctt
     3541 ggcgattggc gttgaggttc gccagggctt gctggaggat cgtgcgagcg aggcgggtgg
     3601 aatgggctgg attatccaag cgttcgagcc gggaaaacgc atccgaaagg tcggtttctc
     3661 ccaccaatcc cgcggcaccc ttgacgctat ggagcacaag acgttgcgtt ttgctgtcga
     3721 ggtcgtggcg agacagagca tcgaggtgtt tttgcagttc ctcgatcaat agtcggcgga
     3781 gttccggatc cagcggggtc attcgcggat tggctttatt ttcgatgggt tagcgatttg
     3841 cagcagcgct cggagcaggg gatggagggt ggggcccagc gctgatcgaa tgatatcttg
     3901 cctggcgtgg tcgccgaggg agccgagggc ttggatgagt tctttggcat gttcggtgac
     3961 tcgggcaaga gcgacggcgg tatggggatc catatcgagc agggagcgtg tgtgtgttcc
     4021 gatgggttga atatcttgta aggtattgtc ggcggcggtt tgcaggctgc cgaattgggt
     4081 ttccaacgct tcgtgggcgt ggcgagtttc ctcggttcga tgaacggctt gattccacag
     4141 cgatagggta tcatccaacg cggtgcgtag ttcttgtacg gcttcgttgg cccgctcggt
     4201 caaggaacgc acttcatcgg ccatgttcat gagggtgtgg cccgcgggat cgccgagacg
     4261 tgctccttcg agaccgacat tcagggcgat gagccgtaag cggtcgaggt tgtgggcaac
     4321 ttgatcgatg ggatcttgga tttcggggct gcgattcgcg gcttggcgaa tgagatccaa
     4381 agcaccgtcg aggagaactt gctggcgttt ggtggaagcg agcaattgtg tggtgagttc
     4441 gacggcatgg tttgcggatt ggtgggcgcg caggtgtgct gtccacagtc tcgccacttc
     4501 cgtctggtcg gtaggtttcg aggtttgtct ggctcgtcga aaccaacctc gtcgcacgcc
     4561 ttttgtcatt cggacctctc ttgttctccg aggcgagcgg ctcgccacag ggcggcctcg
     4621 atatcgaagg tgagttgccg cacatccagt aagcgtgcgg atttattttg gaattttact
     4681 tgcccctcgg ttagctcgaa cactccggta gccaccacgc gagcgccgaa caaggcgacg
     4741 ggtccccctt gggtcatgca catgatcatg tcggaggcgg gtgccgaacc cacctgaagt
     4801 gccgtagcga cctgctcttc ggtcagcacg agtccgatgg ccggggggac catgcctgga
     4861 acgggcaaaa caggttcggc gggggcaatc cattgcgctt cttgggcgga gacgaaccac
     4921 caagattctc ctgcgtcgag caccattcct gcgcacggtg gcatggttgg gtaccttatg
     4981 gtgggttggt agcgatagga gctagcgtcg ttgcttcgtt caagcatcga agccggtggg
     5041 gggagcctat tcgatctcgt ctatttcgca acggcgctca tcgttctcga gcgtggtggt
     5101 cgttggcaag gccatcgaaa tgggggggcc gaggctcacg gatgcaggcc gcggtggccg
     5161 aggggttgta ccgaacaaag ggcggttgaa gtccggacca atgctgagtt ttgcgcgagc
     5221 ctgagattac ggggggagcg gcttttctgt gttgggttcg agagagacga acagcataag
     5281 ggcaaagcgt gctacagggg ggatggtcga catcgcgcat gaggaagacg aagcatggcg
     5341 gacaagagag tgtcaacgaa gagcaagaag tccacggggg ccaaatcgaa gaaagcggtt
     5401 cgttcggaaa ggcgtttcat gccggagcgc tcgaacgcat cgatcgccac gatggccggg
     5461 acgattgtgg gtggattggg tgtgggggct ggtttgtatg ggcaatggat gacgaatccg
     5521 acgatggccg tcgcgccatg ggtattggcg gctggatcgg cggtgcttgc cgcggtgcta
     5581 ttgtggggag atctggaagg aaccgtcatt cgcgtcggag acgcgggcat agtctgggag
     5641 cgggacggca aggtgaatcg tcgtgttgca tggtgtgata tgcgggaggt ggagattcgc
     5701 gacgcaatgg ttcgggtgca gttggaaggc gaagtgatga cgttcagcgt ggcaagccag
     5761 cccttggcgg ccgcgtgggt ggccaaagaa gccaacgcac ggattcccaa acgatggaag
     5821 gcgagcaagg atgtgattca atccttgccg aagacatcgt cggacgacgg agaggggctt
     5881 gtggtcgagc cgcttcaggt cacggggcgg tcctgtcgag cgagtcagcg aacgatcacg
     5941 ttcgagcgag atgctcggta ttgtgttcga tgtggtgaag tttatcacaa ggacgatttg
     6001 ccggatcgtt gtctgacgtg tgatgcggat ttgcgagatg cgcaagaagc atgatgtcta
     6061 gggggcaagg gtcgtatcgg cccgatggtc atgaaaaacg tattggttgc ggtgatagga
     6121 gcgttgggga agggacagca tgcgtgagcg ccaacgggtt gggtggatga gggcgagaga
     6181 catcgaaacg caacacaagg gcgatgggaa tgaaagagtg ttgcgtgacc atccatccca
     6241 ttttccatgg gaagcagagg cctaatcaag cgtattggcc gtggattcag agacgttcgc
     6301 accgatgagt cgtggcttca gggtgcggta gtccgtggtg cgaagctact tcgaagccag
     6361 gagcgcgctg atcaacgaca aggggggtca agagcgagaa tcgcatggtt ggatgcgcag
     6421 gaaggatccc gcaacattag tgtaattatt tgaaactaca cataaatttg tcagcccctc
     6481 acgccatgct cacggttatt ttggctgctg atggaacgtc gacctttttc gattgtggaa
     6541 agaaagggtg cgtggtgctg tgggcgcagg ggttcgtagg tgctggggcg tgtcatggtc
     6601 gtggggttga cgaggcgcag ggatcggtct gcggaaagca ttggggaatg ttcatgggat
     6661 gtggcgttgg tttgcgaggt gggtgaattg gcaagggcat tgatgggggg tgagtgaggt
     6721 tgggggaggt atcgaggtag aaaggggatg tgacttcggc gacggggagg cgagtaggag
     6781 gcgtggaaag gcggaggaaa gctggttcct gtcaactcgc gttgacaccc cgacgcgcta
     6841 cccttagcct gcccgcgtgc ggtcgtttcc atgggacacg gcttcatcgt agaggatcaa
     6901 gcgaaacatc cggtcatcga ggatgaccat cggagggcaa tcatgctccg caacgtgcga
     6961 aagatcaccc cgctgattcg ttctttggtg ctaggcgtcg ctgccgtatc gacggtgtcg
     7021 gtctcatctg ccgtgatgat ggggtgcgag gacgacaaac agcctgagta tcatgtcaag
     7081 cggttgaatg acccagcgct acgtcctcag gcggtcaagc gtctcgtgca attttttgaa
     7141 gatgcgatga cgcgggcaga caaggatcgg acgaagccga atgtgaagga gttgttggac
     7201 aagatcgttc caccgttgac gcagacgtat ctcgagggcg acacgattga cgatcgaacg
     7261 aaaggcgaga tcatcaagtt gctggcggat tcgcaagacg agcgagcgaa gcccgcgtgg
     7321 atcaaagcgc tcaaggatta caagcccaac gtgacggagc ctgacgttcg ggcgtgtgcg
     7381 aaggtcattg cggagacggg ggtcaaggat ccggaagcga tggatgcgct catcaatgtc
     7441 ttcgtgaagt tcgaagcggg ttcccccaaa ggggcgctca tttatgtcga tttcaagaag
     7501 gcgctcgtcg atatttcctc tccttcgtgg aagtcccagc ttctcgagcg gttgaatcga
     7561 ccgatggaga agctcgagca gagtgatcgt accaacgagg acaagatcac ggcgtatcgc
     7621 aacgagcagt tctggcaagt gacgtccgcg gaaattcttg gagaaatcaa ggctacggat
     7681 gcgatcaagc cgttgttcaa gacggtcatc gatccgaaca aggcggatgt ggcggccacg
     7741 gcgatcatgg cgatggtgaa gatgggcaag gattcgatga acacgctcat cgacgcgtta
     7801 ttgggcaagg atgcggagct gacggagttt gctaaagcga aaatcaaggc agagaaagag
     7861 gcattggtga ctccggttcg gtctgccgcg ttggtgattg ggacgattgg tcgagcggat
     7921 ggtgtgcaac cgatgctcat ggctctggac aaggcggaca agtcggatgc gatcacgcaa
     7981 gcgattcttg ctcgtgagct atcgaagtgt cctgtcacgc ccgagtccct caaagcattc
     8041 atcaaagtat tcaatgacat gccggtttcc gcgttcattc ctccggggct tacggcggtt
     8101 acggtattgg cggaatccat tgcgcaattt tacgatccga gtgtcatcga cacgttgatt
     8161 gcgcgggtgg acaacttcaa gggagacaag caagacaagg acttgcttcg cgattccgtg
     8221 cttccgacga tgatcaagtt gatgcgaaaa gatcagatcg atcttgtgga aaaagcgatc
     8281 ggccggtggg cgcctggaaa ggacgacgcc aagctcgaga aagaggcgct tggaaaagcg
     8341 aaggcggttc tgagcgcatg cgatgacaag ctggagtgtt atctcgccaa gctcgaggaa
     8401 gccgcggtcc aggaaaagaa cgagcaattc caagggatca aggcgtcgta catgcttgga
     8461 atcctgggca acgacaaaac gcgcatggag atcgttcgtc gctttcctag catcaaaaac
     8521 gctgccatca agtatacggc gggacaagcg ctggactttc ttgccccgaa gggggatcag
     8581 gccgcggcgg acgaaatcga caagcagctt cagaccaaca ttgccaaagg cgatcagaat
     8641 ctcatcgctg gggacgcgcc gctgcgccag atccgcatcc gccttctcgc ccgtctttaa
     8701 ctcatgattc aagttaccgt ccactcgggc cttcaggctg gtcagtggct gacgttcgac
     8761 gaggatgtcg tgacggtagg acgtgcgtcc gacaatcacg tcatccttgc cgattcccat
     8821 gtatcgggcc accacctgcg tattgtccgt ggtcctcatc gcacgctcat cgaggacttg
     8881 cattcgacgc gtggcacgac ggtgatcatg ggagatgagc ggaagcgtct cgatgagatg
     8941 ccgaatcgtt gtgtggaggt ggacacgggg tgtcgtatcg aagtgggaac gggagcggag
     9001 ttgacgttga ttgaagttcg gttgttggag tcggacgaag cgcgggtcgt atcgatgagg
     9061 cgcatcgagg agatcggcgt ttcaggggag aatctcgaac gcgatcttca gatacttccg
     9121 cagttgtacg cggcgcaaaa gcgtttggac gcggcatcgg atctcgatga agtattggaa
     9181 gcgacatgtg atggcgtgtt gcagctattg ccgcgtgcga cgcatgtgac ggtgacgtta
     9241 cgcgatgagg aggatagttc tcaggagccg gtctatgtgc cggttgtcac gcgggagcgg
     9301 ggaacggatg gttcgggggc agcgtctcgg ggagcgattc cgattgcgcg aagcgtattt
     9361 cgcaaagtgc tttcggagcg agcggcggtt cttgcggccg acgcaccgcg ggaagtagcc
     9421 caggcggaat ccattgttgg agcttcgatt cgaagcacgg cggcggttcc tttgtggcgt
     9481 ggtgaaacca ttcttggggt catacaagcc gacaatcgtc aggctcctgc catgctcagc
     9541 gcgttggact tggaaattct cgcgctcttc gcatccaacg catcactggc ggtatccaac
     9601 gctcgattga tccgaaggct tcagatagcg caagaacagc ttcgcaaaga aaacgatttt
     9661 ttgcgaaccc gcgaagagac gcgcacgcgg gcgcagccga ttttggggga gagcgaagcc
     9721 atgaaagccg ttcttgccca gctagacaag gtggttgaca cccgtgtcac ggtattggtg
     9781 gaaggagaaa cgggtacggg aaaggagctt ctcgcgtcgg cggttcatta tcgttcgaag
     9841 cggcgacaca agttgttcgt ggcgcaaaac tgcgcggcgc ttccggaaaa tctgttggaa
     9901 agtgagctat ttgggcataa acggggtgcg tttacgggag ctgtggacga caagaaggga
     9961 ttgtttgaca tcgccgatgg ggggaccttg tttttggacg aggtggcgga gatctcgttg
    10021 tccctgcaat ccaagcttct tcgtgttttg caggaaggcg aggtgcgacc ggtgggagca
    10081 tcgcgcacgc attgggtgga cgttcgaatc gtggcggcga ccaaccgtaa tttggaacaa
    10141 gaagtgcgcg agggacggtt tcgggaagat ctatactacc ggctcaaagt gtttcctctt
    10201 cggtcgccgg ccttgcgcga gcgaagggag gatattccat tgctcgccgg agcgttcatg
    10261 aagcgatacg catcggaact cagcaagacg gtgggcggtt tcagccaaca agccatggag
    10321 ctgttggtgg cttacgactg gcctggcaat gttcgcgagt tacagaacga aattcagcgt
    10381 ttggtgattc agctcgacga aggagcgttt gtggttccgg agttgctttc ggagcgtatt
    10441 cgttcggtcg agggcgtcgt gggaaaggct ggtgtgggaa agggaacgtt gcgagagatg
    10501 atggatcgag tcgagaagta cttgctgatc gaatcgttgc gtgagcatgg caacaacaag
    10561 accaacgcgg cgaaaaccct tggaatcacc cgggaagggc tccacaaaaa actccgggct
    10621 cacggccttt gaagccgctc attgaatgcc gaattttttc aatcgtcgat agaacgtgcg
    10681 ccgcggaatc tggcaaagtt ccgcggcctt gacgcggttc cagttcgcac tggccagggc
    10741 ggcaagaatt cgttcctttt cggaggcttc atgggcggac agcgtttcgc ttgtgccgag
    10801 gtgtttcctg gcaccggtgt cgactggatg cacttcggag atgaacgatg gaggcagttc
    10861 cgaggagcga gagtcgctcg ttcgttgcac ccctgccatt tcgaaatcat cgatttcgag
    10921 ttgggctcga tcgctcatga cccaagcgtt gagcagggag ttttcgagtt gtcgaacgtt
    10981 tcccggccat ggacaagcgc atagcagtcg aagcgcatcg cgggagatcg tttttcgttc
    11041 ccttcgatat cgcgcggcga agatattgag gaagtggtcg atgagcatgg gaatgtcttc
    11101 ggcgcgttct cgcaaagagg ggagttgcac atcgacgacg cgcaagcgat agtacaagtc
    11161 ttctcggaac gtaccttcgg cgaccattcg ttccaggtta cggttggtgg cggcgatgac
    11221 acgaacggaa acgcgttctt cgcgagcgcc acccacggga cgaacgaggt gctcttgcag
    11281 cacgcgaagc agacctgctt gcattttatg tggcatttcg ccgatttcat cgagaaggat
    11341 ggttcctcct tccgtctctc gaaacaggcc ttttcggtcc cgatcggcgc ctgtgaacgc
    11401 gcctttcgta tgaccgaaca attcactttc gagcaagtgt tctgggatgg cgccgcagtt
    11461 gatgcccgta aacggctttt tgcctctggg tccaccgcga tggatggctc gcgcgaccat
    11521 ttcctttccg gttccgcttt cccctgtgat caacacgggc acatccatgt ctttcacgcg
    11581 atcgatcagc gcatagacac gtcgcatttt ttcgctggtt cccacgagtc cctggtaacc
    11641 aaagtgaccg cgaagcaccg cgcgtgttgc gtcgagatcg cggcgagtgg attcgagttg
    11701 cgcggttcta tgtccgagga cttcttgtag gcgtgcgtgt gctttttcga gttcggcatt
    11761 ggttttcgcg agttcttcgg ccctacgttg gttttccgcg atgagatgag cgttggcgat
    11821 ggcgacggcg gcttggtccg cgaaggccat caacgtggga agttcggtat gaaaaagagc
    11881 accggggcga tggcgtgttt ccacgtacaa tgcgcccgcg gttcggttgt ccggcgctcg
    11941 gatgggaacg caagccacgg attgcacatt gagttgatga acggaaagat aatcggccat
    12001 tcgttcatct tcccgggcac tgagagtgat gaccggttcg ccggtggcga tgactttctc
    12061 tgcgatgttg gaagagaatt ttgcgtgaga ggcgtcgcct tgccgatttc gcgagatatg
    12121 cggaacgagc acgccgtttt gggcgagaat gacgaagcct cgttcggcat gcaccagaga
    12181 gatggcgtgg tcggtcactc gttcgagcag gggttccaac ttgtgctctc tggctaattc
    12241 cctattgatt tcaaggagtt tcgttaatct atcttcgacc ggcaacgcga tcatggtgga
    12301 cgatatgccg gtaccggagg cgcgggagcc cagtggccac aaggaattgg caacgatggc
    12361 gttggagcga gggagctgca tcccaagcag gttggttcga acagcgcgtc gccgtgggtc
    12421 atcccaaaag acttctctca agtcgcgagg caggcgggta gcaatttcct ccagcacggc
    12481 cagggcttct tcggcgtctt ttcgtgcggc caagtcctga cccatttcga caaggaggcg
    12541 agagcgggct tcgagggttc tccaaatcca ctctttttgt cctgcttctc ttgcggcgag
    12601 caaggcggca ttgaaggagt tacgggcgcg atcttcatcg ttttgggcgg cggcgacggc
    12661 acctcgggcc aagtgaagca aagcgcggtg cgcggtatta tcggtgagca gagacatggc
    12721 ttgttcgatg tctctggaca gggacatgag gtcacgggaa tcggagcgga cagagacgag
    12781 cacgccttcg agtatggcct cggcggcatc cacgcttcgt ccgagcgctt cgtattggga
    12841 agcgcatgag gcataaaact cgacagctcg gtccagttca ctggttcgag cggcgagttc
    12901 ggcctgcata cccgcgagtt gtgcacgatg attgggcgct aatttttcac gttgttccgc
    12961 gagggaagcg aggctcgagg tagcacgggc gtatcgtccc aaatagatat ccaaatgagc
    13021 caaattcagc agtgcttgtc gtgtggtggc gccacggctc gcgcgacgtc ccatgtcgat
    13081 ggcggcttcc agatgttcaa tggcatcggc gagatcgccc tcgatattgg cgagcacggc
    13141 gaggttgagg cgtgtgttgg cgacggcgcc cgcgtcgcct gcatgctcgg cgcattcgag
    13201 ggcttcttcg aacgtacgac gtgcttctgc cagtttatcg ccccgttgca gcgcaacagc
    13261 caaagagccc acgacgatgg actcgacgcg tttgtccccg agttggcgtg cggcggcggc
    13321 ggcatccata agttcttgtt ccgctcctgc gtggtcaccg ccatagctat gcaacaatcc
    13381 gatgcatgca cgagcttcga gcgtgacggc gattcgttca gcgtcagtgg cctcgagatc
    13441 gatcgcctgt cgtgcgtaac tcatggattc ttcatgacac cccaagcgta gcttgcagcg
    13501 agccacgttg agcatccatc gccgtcgagc caaaccttgg gaaggaatcg aggattcatc
    13561 gatggattgg agcgtggcca aggcttgtgc ggcttcgcct tggccgagtt cgagccgtgc
    13621 tttgcaaagg gcgtattcca acggatcggt attcgacact tgttgaagtt tttccgcggc
    13681 ttcttcgagt cttccttggt tcagaagccg atccacttcc gcgatatcgg aagacaaaag
    13741 cggatgcgac acggtcgatt cggtctcttg ccacatctca tccatctcgc tgatactcag
    13801 tacgggccga ctgccaattc gtcgcacaat agctcgtaat tttccgggtc tgccttccgc
    13861 gcgccgaatc gtatgttgaa ccagggaatc ggagagcgaa gggatgaggc gtcgaacaag
    13921 ctcggccgcg gcttcgtgat cgagagcagg catcacgaag gtttcgacgg gaccaccgat
    13981 cgaagtggcg acatgagggt gcgcgaccaa tacgattttg gctccttttt cgcgggcttg
    14041 ggcaagacgt tgggaatcgg tttccgtgag agattcagcg ccatcggcga gcacgacaac
    14101 gccttccagc gtgggatacg cgcccaattc catttccagt tcatcggtcc acggcacgga
    14161 ttgctcacct tgaatccaag ccacgtctct tccctctatg cccaaggtcc aagcgagacg
    14221 tcgcaacagg gtagatcggc cggagcctat ttttccgacg agagcgatga cagaaccggt
    14281 gaccatggtt gcgattcgag acagcaacaa gttggcggcg gcatcgacac cgacgatcgg
    14341 ccaaacgagg tctacggcgt gattggcgga aacgtcgggc aagtgagccg cgaggcggat
    14401 ggcactggcg atttcgtcgg tgcttgggtg gcgttgggag gggtccgtat gagtggctcg
    14461 cagggcgatg tcgtggaggt tggaaaggat agaggggtcg agttggtgac cgcaggcttc
    14521 gaggacgtct cgaagggtag caccaagggc aaaaatctcg gatcgcacgg taatcggcat
    14581 tccggcaaag agttccggag cggcgtagcg aggagtcaag ccctcggggc gggcaccttc
    14641 ttcgcgccag ggggcggcta gtcccaagtc cacgagagta gcgccttgtg gggagacgat
    14701 gatattggcc ggttttacat cgccgtggag caaggaagcg cggtgcaatc cggtgagttg
    14761 attggcggca tgaagcaagg cgttcaagca ggtatggggg tccggtttgg gtccttccat
    14821 catggaaagc aggctatgac cgggcacgag ttcacggacg aggtaggggc gaagggtacc
    14881 gggtagacga ccgaagcgca ggactttggg aacgccgaat ccctcgaggc cagacagggt
    14941 cacggcttcg cggacgagtg cttggacttc tttttgggag gcgttttcag caagggcttt
    15001 gagagcgacg gtttctcggg tgagtcgatc tcgtacggcc cagacttgcc ctccaccgcc
    15061 ctggccgaga acggccactt gttcataacg agcaggaaat tgtaaggaca tgggtacctc
    15121 agcgcagtcc aacctgtcct gacaggccaa agaaccacgg accggggaaa gacagacccg
    15181 tatcgagtcg aaggacgaag tcacggctag cgttccagcg aacaccgacc tggggcagtg
    15241 taatccatgg aaacaggatg ggtttcgcgc caccgtggag ccaattgggc tcttgataat
    15301 cgttatagtg gtcgttggac gcgtcacaat aatcgccatg ggcgcgtcca gggccggggc
    15361 atttgacata cgttgtggga tcgcccggct gaccgtcggg gggataggct tggttacgaa
    15421 tcagatcgcc aaacaccacg ccaatgcctg cgccggcacc gtacgtcaac gtaaagcgcg
    15481 ggtctcccag cggtttcgac cacaaaaaat cggctcctat ggtcaaggtt ttaatgtggg
    15541 aacgaacgat ttcgtacgat gtgtcgggat cggacttggc tttgaagggc acgtcgccca
    15601 tgccatagga gatgaaaaca agccaaatat ccgtttcgaa cccatgtttt ctcgtcacat
    15661 attcggggcc aacggaagga gagaccacac cttttccgcc gtcggcgccg aatagatcga
    15721 acatccagct tgggatataa gcaattcgtc cgcgcagccc gatgaagcgt tggtcttcgg
    15781 catcgccttc gtttcgcgag gaatcggcgg ccgcggggga ctgcttgggg ttatgttttt
    15841 cctgggggaa agcagccgtg ccgtcggtgg ccgcaagcga ttccgagggg gattgcgagg
    15901 gagaagccgg gtcagtttgg gcgtcgacag ccgtcgcaag ggtagcgaag gagaaagcga
    15961 taagcggggc aacaacgcaa tttttcatgg acagcccaag agggttcgga gttgtgacaa
    16021 ttcgtgccac aaggctgagg gcgagtatac atgatttggt gagccatggc atcctgggca
    16081 cattctgacg gtagttgtgc catgacccat tccaaggatt ggcaagggcg aaagaggatg
    16141 cgcggggtgt tacgggtggt atctcacgat aagggagatt gaagggtgtt ggacgaagga
    16201 tcgatcaatt ggaatcgtct cttcgagagc gagcgtgttc gagttctcgt ttgaggtcgg
    16261 cgagttggtc ttccagggaa cgaacggtac gacgcaggtc acggacttct tgttcggtgg
    16321 ccagggacat gattttggcg acacggtcgg cgtgttcatg ggctgcggtg gtaagacgac
    16381 cgggaacact catcgctgcc atcatggctt tcatgagtcg ctcgtcttgc atgagcttca
    16441 tgacgcgagg gtcgctcatg agtttcatac cttggtgtaa cgctttcttt ttgaggctca
    16501 tgcggtctcc catcgggtgt gagagcaacg catacgccgg acgtcgcgtt gtggcaaccc
    16561 caaggtgaga gagcggtggg ctggtcggat gtcctcggag tattcgttca ggggtgggag
    16621 ggcgaaagac ggttatccat aagggattgt gatgactagg gaggaatggg tcgagggcga
    16681 atcgcgaaat ggctgtggaa gatacgacac gaggatcgac gacacactat ccgaatacgt
    16741 atacttagaa gggtgacttc gatagagccg aaggggagat tgggacgcaa acggcgggcg
    16801 tacgtaggtt ttggaaatcg tgggagaact ggcggaaaca tgggagatga tgaggagaat
    16861 ctccccttct tcgaaagagg aataccgcga aggccgaagg cggtgaaaca aggaggcgaa
    16921 gcaggaaaga aaaagcgata ggtcttggga gattaaaccg gatacggata tgacgggaag
    16981 aggaaaagga cgaaggcttg gaattggata tctcgtgctc cttccttcgc tagccacagc
    17041 gcttggttac aagcgtcaag caacgcgttt ttcgtgagaa aaagcgaatg aacccatcgt
    17101 tgtccatctt tgctcttgtc ttgttcgtga tgactgtgga actctgtcaa cccatgaacc
    17161 aacgagatta tcaagcacaa gtgatcgatg cggctgaccg tttgaccgat gaactcctag
    17221 ggtattttgg ggagttgttg cagagtgtcg ttagagacgt cacggttggg attgccaata
    17281 cggcaccatc cacggcatcc aagaaccggg cagtcgccga aaaaaaagaa cagaaaagtg
    17341 cccccaagcc ctctcgaccg tcggtttcga aaaaaaaaca ggtagcaaaa aaaccgtcga
    17401 ctcaccgcct ggcatcctcg ggacgtcgtt ccgcccaaga actcgacaag gacgcggcgc
    17461 gcgtcgttcg gtatttgaaa gaaaagaaag cggagatgcg cattgagcaa atcaacacgg
    17521 cgcttggaac gacgacgcca caactgatgc gacccatccg gcgattgctt gcgtcgggcg
    17581 agattcagaa gcggggaact cgcagatcga caacgtattt cgttcccgca tcctcattgc
    17641 acgactgact gaaagagagt cagagcccgc ttttgtccta catttggttg ggaagcagaa
    17701 cgaatcctac cgtggcatcg ttgtgcggtt tgcatgccgt gacgccgctg agcaaaggtt
    17761 ttgcgcatcg ttactctgta gtgctttcgt gagggaggca tgcgtgggcg gaagggagaa
    17821 ccgcaggcct tagggggcaa acggtggtgg aagcaaagcg gtccgaagga aggtggcctg
    17881 ttgggaatcg atggctcatc cctaggcgac aattagcgta actactggaa attacacgag
    17941 atgtatccct tctaggggga agggtgttgg atctccgtgg cagatttcga cgattcgtct
    18001 tgtgcgttca acacgattat gggaaagtcg agtgccatga cgcgagatga actaagagaa
    18061 aagattcgtg ctgcggccct acactttacc gaagaggtaa gtgctatttt tggggaagcg
    18121 tttgcttccg tggcagcgga gttccggtcc ggacccatgg cggcgagtac aacgccaaaa
    18181 aagccaacgc cggcacctaa gccaatcaaa gctgccaagc cgcaaaatac caaaacaacc
    18241 gtggtcaaac cagcggccaa aacgcctacg acggccaaga aaaagcccgt accgagtaag
    18301 cgtattcgac gctccgccaa tgagctggga agggtgggcg aagaggtggt gaagctgttg
    18361 tcgaccgctc cagagcccat gcggatcgag gagatcaaca aaagactcgg tacgaccacc
    18421 cgccaactca tgcggccgat tcaaaagctg atttccgacg gccagatcaa aaaagaggga
    18481 gagcgtcggg caacgattta cttcctctaa tcacgcccca ggggtttatc gctccaaagg
    18541 cgacgccgaa cctgccggtg ctctatcgct agtggagtac caccgtcccg ggcgaaactg
    18601 tagtttagcc cggtaccccc gcaataccca gctctccgct ccttcctgcg agataacacg
    18661 ttgataatcg gcttctgggg tcaatacgaa gtccggctgt gcgtaatggg ctttcttttg
    18721 cggttgtacc cattgcccct cttgccactt ccaatggcgt cctgaccatt gccattgacc
    18781 atccacccag acggattggt catcgggggg cggtggtagc atctgcacat gaacgatatc
    18841 tggaggtttg ggtaccgaca gaaacggctc tgtagcgtca tggggccctt gtgggggaat
    18901 tggtgtgaca gacgtacagg aggcgatgag agccagacac agagacaggc aagcggcaac
    18961 caggacgcga gagcaagacg gaagcggtat catggcgcga gccatgccat atactgcgac
    19021 aaaagagctt caccgaccag catatattcg atgatggcga gagccagaaa agggccaaaa
    19081 gggatcctgg ctcccgctcc gccagcggaa ggttcgaaaa tcgggtcttt ttgtagttct
    19141 tgctcggctt gttggcgttc gaccggatct tcgatggcgg ccaccgcggc aagcagttct
    19201 tctcgctccc gttgcacggc ttccggttcc tcgagtcttc cccgaaccaa tagaacaacg
    19261 acggcggcga gggttccttg cacggaaccg cctagcagag cgaaaaacat ccctttccat
    19321 ccgaaccacg cgcctgcaac cgccaggagc ttggcatccc ctaaccccat ccccgttcgt
    19381 ccacgaatcg cgcgatataa cacaccgaac acgagccaaa cgacgacaaa tcccgcgata
    19441 gaagcggcca acgattcggt gaaggtcaac gggggccgaa gggaaaagga agccaagcct
    19501 acaacgatgg caccgaggga catggaatcg gggatataca gatggtcgag atcgatgaag
    19561 gctaccgcaa tggaaaccaa gccaagcgca aagtaggtgc agaaagtcag agcgcctagc
    19621 catagggggg tatcgggagc catggggcga aggacggttt gcaccaaggc cagggctagc
    19681 aatcctccgg ctgcttccac gagggggtaa cgaatgggga tggcggcacc acagcaagca
    19741 gctcgccctc gtaataacaa ccaactgacc acggggatat tgttccaagg gcgaatgggc
    19801 gtttcgcagt gagggcagcg agaagcgggg cgaaggatgc tctgtcctcg aggaaccctg
    19861 taaatgacga cattcaaaaa actgccccaa atcaagccga aagcgagggc aaatcccaga
    19921 aggaaccaag ggggaagctc aacgagcgtg ggcacgggca tacctcacgt tcgtgcgggc
    19981 acgggaatgg gaaatgcact gggcaataag gtaggaaggg gcggaaggga ggaaggtaat
    20041 tgcgtaggaa gcaaggtagg aaacggaggc atcgcgagcc caccgtcggg caaaggacca
    20101 gcgtctggtt tgggcgggtt gaccgacgga accgaaggga ctgaagtagc cgctggggat
    20161 accgcggggg aggaaaaggg cgaaaccgct tcggagggca ccgcaccagc cccttgcgcc
    20221 tctccctgtg tttgactgga aaaatcgatg ggatcatctt tttttgagca gccgaaaaca
    20281 gcgaaacaca ggcaaaccag aatcggagag cgaagcctca tctgggggat cgtagcatgt
    20341 tcccttcgaa ggcgagcaac tccctgtacg atacgtttcg agccattgcg ccaaggatgc
    20401 atggaagggc tcgttttcca acaacaagca tgaagtgcca ccgggctgcc acgaagccta
    20461 ccgaacgatc cgtaagcatt cgtcgcgtcc ttgctattcg tggtaccgta ccgcacaatg
    20521 gacgaggcaa gggtgcgggg ccgaaagact cgaccatcac gcccgaacac atcataacca
    20581 atggaaatca atagactatt ttaaaaatcg tcgacgatgg gttgcggtcg aattggtaat
    20641 caaccccacg ttgcgcgcgg atcatccaat gaaaggagag aaagacccca acgaattcgc
    20701 tcgaccgctc gagccttccc ctcggtccca agctccatcg tcacggtggg aacgtctggc
    20761 tcggctcagc cttcttgcgc cgaaggtatt gccggttgcc ttggaaggag ccaaacgcgc
    20821 ggtgggcgta tcgcgttcgg agcagcagga gcggttggcg cggcagcgaa cgtacgagaa
    20881 tgcgaaaaaa gcggggacgg cgcttttgaa aacgcttggg gagatgaagg ggcttccgat
    20941 gaagctcggg cagatgttca gctatatcga tgggctggcg cctccgggtt acgaagacaa
    21001 ggttcagcgt ttgcttcagc aattgcgtga taaggccccc cctctttccc cggaagcggc
    21061 tgaaaaaatg atctggcagg agtttggggt gggaccggaa cggcttttcc gtcattggga
    21121 gcccgttcct ttcgcggccg cctccatagg ccaagttcat cgggccatga cacgaagagg
    21181 cgatgaagtg gctgtgaaag tacaataccc cggcatcgac aaggcggtgg aaaacgatct
    21241 caaaagcttg tccatgctcg aagtcgtcct ggctcccttg tctcggcagt accagtcgaa
    21301 ggaaactctt cgtgaaatta cggaagtttt ccttgctgaa ctagattatg gcctagaagc
    21361 ggaaaatatg gatcgatttc ggcagttatg cgccgatctt cccgcggtgg tgatacccca
    21421 cgtataccat tcgttatcga cgcgtcgggt gctgacggcg cagaggctag ggggaatggg
    21481 gtatgagcag tttcgggcga cggccagcca agcgcagcgc gatgcggcgg gggtgaccct
    21541 gtggacgttc atgatgcgaa atttgctggt tcatggtgtg ttgtacgccg atccgcaccc
    21601 tggcaattat cgatttttgg aggatgggcg tattgggttt ttggattttg gatgtgtgaa
    21661 ggtgttacct accgccatgg cccaagggct caaggattat atggccgcgg tcatggatgc
    21721 aaattggccg gaattcgatc gtctttgcca tgagttcatg ggcatggccc ctggtgacaa
    21781 ggcttcgtgg gatttgtatc gcagctacgc gatcgaattg atgatgccga tcgcgactca
    21841 ttcgacgtac gagtacacgc ccgagagcgc gaaagaagcg gtgcaatatt tggtaagagg
    21901 acaccgcaag ctcatcaaag aagcggaggg aggacccaag cttcccacgc cgatgcacat
    21961 gcccttcgat cttacgttcg tgaatcggct gcagtgggga ttggcttcga tactgggcgg
    22021 attgaggacg aaagcccgat ttcgacagat tacggagccg tgggttcgcg ccaagctgga
    22081 gtcgcctttg tgaactggat caaagggcat tggtggtttt tgctaagcgt tgccttgggt
    22141 tgcacccgtg cggaacctcg ctccaacacg ttgtcactca atggttcggt ctcgacgatg
    22201 agcgtcgaac cagccgtttc ctccgcgaat tcttccgcgg cggagattac cccaccgcaa
    22261 gagtcctggg tcgaggcggt tcgtctcgaa gattgggctc ttgcagcgtc tttgctcgat
    22321 aagcatgtgg attgtagcgc accgaggatg cgatatgttc gcgccaaggt tgccatgggg
    22381 caagagcagt ggtcggtagc gttgacgttg ctggacggtt tggagtcggc gcttccgcag
    22441 gtggcgacag atatcgcgct acagcgagca gaagcgcatt ggcatggcgg tcaagcggat
    22501 ctagcagcgc agtactttgc ccagctcaag accgtacggg cgttgacgaa agcttcccag
    22561 gcgtattttc gagccagcca agtcaagtta gcacgcagcg ccatcgatcg agccattcaa
    22621 atggccggca cgtcgagccg tgaggatggg atcgaagctc gccttcatcg cgcgatgctc
    22681 gcacaaacga tgggcgacaa agccacggcg atagcggatt tgcgtttcgt ataccagcat
    22741 gcacgcgatc cccaagtcag tgccgaagcg tggaagacgc ttcgtcaggt ggacccaaac
    22801 catcgtctga cttcctcgga ccgtatgcaa cgcgcgtgga gtcatgcgaa tcgtggttat
    22861 tcggagccaa cgcggcaaga gatcgaacaa gccaaagcag ctcccggagg gccacctccc
    22921 cgggtccagc agcttcttgc ccaagccaag gcgtattacg aaagccgcaa ggattatcgc
    22981 caagcggcgc aaatctacga gcaagcggcg aagcttcgcg gccctcatgg ggcacaagcc
    23041 ttgtatttgg cggccaaagc atggagccgg gcggaggata acaatcgagc gctcgccttg
    23101 tacgaacagg tcatccatca gcacaccgcg tcgtcttggg ctgaacgggc tagctacgac
    23161 gcccctcgac tctaccgttt tcaacgtcga tggcctttgg cggaaagagg ataccgacgc
    23221 tacttgcagc gttatccacg agggtcgttg tccaaggagg cgcgatatga gcttgcgctg
    23281 tgtttgttgc tttcagggca gcctgggcaa gcgcgacctt tgtttgagtc tctcgccagc
    23341 ggggagaaac aggcattcga agcggcctcc tttcgttatt tgggagcggt tgctgctttt
    23401 ttggaaggga atacctctct cgccgcttcg gtatggcgag acacgatttc ttcccagccc
    23461 ttgtcatggt tcagcctgat ggctcaggcg cggctagcgt cgatgggaca acccacgccc
    23521 ccgattgccc ttccggctcc ttccgttcat gagctgccac cgatggtggt gaaccttccg
    23581 ccgacggttc aatggttgcg cgacctgggg ctcgttcgcg atgcggaaga agcgatgcgt
    23641 actcaggaag aagcttttca gcgcggttat ggtgatcgcg gcggagaagc cttgtgccgc
    23701 gcttacgggc agcttcagac ggggggcagg ttataccgca tgggtcagcg gcacgctccc
    23761 caacgccttg ttcaactggc accgacagca tcgaatcgct gggcgtggga atgtctattt
    23821 ccctatcctt acggaccgat cacgcatcga tgggaaacgg agcacggcat tccttcaggt
    23881 t
//
LOCUS       DAOSOR010000015        23863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_2138_length_23863_cov_4.564474, whole genome shotgun sequence.
ACCESSION   DAOSOR010000015 DAOSOR010000000
VERSION     DAOSOR010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 23863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23863
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2138_length_23863_cov_4.564474"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PK710_01540"
     CDS             complement(<1..375)
                     /locus_tag="PK710_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOT08415.1"
                     /translation="MADTTQDIKLPLEFFEKLPKTDLHVHLDGSMRVDTILDLAREQG
                     VTLPADDADGLRRLLRFGTNYGSLVEYLKTFDVTLSVLQTKESLFRAAYELAEDAARE
                     NVRYMEVRYSPMLHTRRGLRLTT"
     gene            549..2387
                     /locus_tag="PK710_01545"
     CDS             549..2387
                     /locus_tag="PK710_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOT08416.1"
                     /translation="MATAPQNLYKADLRDVMFVLFEQFKLGELLGKEPYEAWGSEEVR
                     MVINECYRFCCEVLGPVNALGDTQGCTIENGQAKVPPCFKTAHAQLYEAGWKAVGVSP
                     HFDGQGAPKAVYMAVEEFITGANTSLSMYPGLAHGAAELIETFGTEEQKARYCHKIFH
                     GTWGGTMCLTEPEAGSDVGAARSKAFKNPDGTYRIEGTKLFISSGDSDMVENVIHLVL
                     ARIDGAAAGTKGLSLFIVPKLRIKEDGSCGEPNDVVLAGLEHKMGIKGSTTALLNFGE
                     NGQCIGELVGGVEHEGMKQMFMMMNGARIFVGIQGLANASSAYLNALTFCRDRKQGSS
                     IDNFKDPNAPRVPIIQHPDVRRMLLDMKSRVEGIRALAIKLSMHQDLARVASDEKAKD
                     HHQGQVDLLTPIVKAYGTDEAYRVCVTAMQAMGGAGYISDYGVEQYCRDSRIFTIYEG
                     TNHIQAMDLVGRKLSQRKGANFQELVGDIAKFVAKHKEHPRLGAPVQLLAKAQETIAA
                     TAMLLFKWSQSGKLKYVDLHANRFLEMMGATTVAWLLLEAALIAEEALQNVQNDHPDR
                     FFYEGKIQAARFFAHNNLPLVPALGAMMQTDDDSAMAIPEEAFSTI"
     gene            2444..3211
                     /locus_tag="PK710_01550"
     CDS             2444..3211
                     /locus_tag="PK710_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08417.1"
                     /translation="MHTIRLSTLSLAACCFSLPASAQSSPPAHDDFQSGGLAPPTAPP
                     PIQTQHPGFQSETERTLDESEKEDAGRNLDWVYFDVEGGYEVVGLETLKSKGLTYDDA
                     QTSDGGLMLGTGLGLRLIYMTVGARARVGMLSLGNLGTINGEFGVNLPLGDLEPSFHV
                     GAGYAFLASPDADRWGGDPTIHGWNTRLGAGFDYYVTPAFSVGARLSGDVLFLSRKAM
                     TLEPTSNLGANARQAASSKGNGIGAAFSASLGLALHL"
     gene            3231..4400
                     /locus_tag="PK710_01555"
     CDS             3231..4400
                     /locus_tag="PK710_01555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOT08418.1"
                     /translation="MTAWIAPASVWLILCAIPCVAWLYFLWRLPPHRTSAFAAWGTAV
                     LGAFMGVASSWIQRFVVELTGLEQAVSTFGAPSGLLYFLVFAAPLAEGAKVLAAWPAL
                     RSAHVDERYDGFLFASASSCGFAASQSAVALLSARIDIDVMLRVMLLLIAHPLMSSFW
                     GHALGKMRRFRIPTGSFILSWTLATLVHGLLLHLTRSTSWFALVAAFPVLGGLAFATG
                     WAARDLLARFAVSARPSRQSMLSLLPSPSLHAVRHALRRTDRPIMVHWIVVGALTTTG
                     VMLTMVVLAVWFGHQVGLDFSAIEREDTTARAIVPLVLITVSILAAFPVSGYLITKAS
                     GSDNVLEPALSATLAIAAVLIMLGIAAPVALVFALAFAPIAFGLACSGAWFGLDK"
     gene            complement(4404..5336)
                     /locus_tag="PK710_01560"
     CDS             complement(4404..5336)
                     /locus_tag="PK710_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08419.1"
                     /translation="MRAQAIFPILGVGLTLLMLSRAAWADGLFMPSTRLSRNDREAIQ
                     QQVARYKTLDPVSFQRLETLQGHRPQVYRQFRSQQPSVLAELQSLGPGAGWALLEAVS
                     FRAPERGEATQREWDAVREGIIIALGEREMPEASPVFRAIFTHENSDWIALRGAAIGL
                     GRLGGESEKTLLINALQRKGQRRDAALWGLRYVRDLRAVDAVVPWLMDSSEPTAKLAA
                     RALGYMGSSWAWRTGRAGKKEYEMPIRGKCAEALLDAFSRRGEALREPIARSLSMVEH
                     PDTLSRVEQVIDSLTNEQERRAWLRLKQRLQASR"
     gene            complement(5333..7246)
                     /locus_tag="PK710_01565"
     CDS             complement(5333..7246)
                     /locus_tag="PK710_01565"
                     /inference="COORDINATES: protein motif:HMM:NF015055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-arginine deiminase family protein"
                     /protein_id="HOT08420.1"
                     /translation="MLERLTMLRVLSMFEPKSPLRFHGSIALGLGLILAQGCSSSEET
                     MDLMKPSAPVSDAGGHDAEDKDEGTQDVQGIDGQDGQGDSDEPVTRRVLGDLVVDANR
                     NGVIEPDSFEEQTFEDQWNESIGAILLANVDDDDGDGVGDHLDEVVNGPEDAKDLARI
                     HVVGFEDVPANAQGIISVDPEAAPWVRLFRVQADEFVLEDPTHVVVTADDLAHGVEFA
                     IEAKFFPTSTAAGAWSGFVDIEHIVTNGHEELSRDQVRMRVAPLVFMHNLMKTEKIWV
                     GGFDGEFIKGLKAAATAAAVAVDVLNYDAPGYANHDFDQWTQDHFEMGYASMPSKEGM
                     HTMLVAFRTPRVKRTSADVVFVEFLGKDFGAIHVHDQPYDQATRSLDSTGNWDTVPPH
                     RAHGVSYPQGRFILGSTAERHPDPVAEEFVQAQRVQPLIRVDTSWLAVGHVDEILSFV
                     PANSERGWRMLFARPKMAVDMLMSLQKAGHGSAKMHEGKWWWGGPASRTVDQVLGNAN
                     LMATNQEDQAILDGILLQLKDELDLQDDEIIDMPFLEQSIPEGSVAYQPGTVNLLHFD
                     GHVLIADPFGPNVGGVDVFKQDLDTRLSATGLVTHYVDDWNTYHRNNGEVHCATNVLR
                     VVPHQHPWWESGR"
     gene            7267..9168
                     /locus_tag="PK710_01570"
     CDS             7267..9168
                     /locus_tag="PK710_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOT08421.1"
                     /translation="MRSSGEQCLLSMDTVTRAVVFSLNTHSVLGNGPMDARGRYDARI
                     RTIEQQTNTLQRRYDLLAWSRLSVFILAMASAIGTLWKDLGSSGWSVTLGASVLFAVL
                     VSYHARIAQQRDRLQAARRHNERGIDRLDGKWVDFEDDGARYLRDEHAYALDLDIFGK
                     ASLFQLLNDTATRMGARRLATWLAGPAQDDTLRIRQQAVQELATLHSWRESLAVAGAH
                     ADDTLPDPEPLLVWMERSPTFKTPTIPRICAWIGTCLVLGAAVLCSVLSLSAWVFLLP
                     YLVNILVSIALSRRLQPIIDAVSERASDLERHGEMFELMERQAFRSEGLVALQQKLKA
                     SGRLASAETRQLARIVSFLDARRNGAFRALIGSALLWDLHCVFALERWQRRVGPSARG
                     WMRVLADLEALSCFATFSFEHPDFAFPSMDPGELHFEAQGLGHPLISDDERVVNDVSL
                     PGPGSLLLVTGSNMSGKSTLLRSIGVAAVLGLAGAPVCARCLRMSACTVMSSMRVSDS
                     LKDGISRFYAELMKIKRVTETAEKTPVLFLLDEILHGTNSRERHVGARSILRMLIDNG
                     AMGAVSTHDLALASLADELPDRVRLVHLQEQVVDDKMIFDYLLRDGVVKSGNALRWMR
                     FVGLRVDEP"
     gene            complement(9210..9830)
                     /locus_tag="PK710_01575"
     CDS             complement(9210..9830)
                     /locus_tag="PK710_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08422.1"
                     /translation="MKKRGVGIVCCVATSLAISTTTWSASAEEGQEEAKASSVQEDFK
                     PVALTLNPLTLILGRFGLNVEYLPAPHHGIMLNPYFSSASAEGSDIKTTYTSFGAELG
                     YHFYLGDCGADGFFVGPQFVFLTSKAKDECKSNGCVLDDAEASFSAYGVAIDLGYQYV
                     ANSGFTIGVGGGAMYLKTSSSVSNTSSTVKFSGTIPRLLFTIGYSF"
     gene            complement(10103..11914)
                     /locus_tag="PK710_01580"
     CDS             complement(10103..11914)
                     /locus_tag="PK710_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT08423.1"
                     /translation="MNPLIRSSAFSKRRAIAWLVAVGLVSAPVSAQPGASTRIFEPTS
                     PGDRFRLVPDAMVRGDGEPWVGWSWDYAYRPRSVFDEREEKGLWEGAEIFTLLGASYA
                     LWNRLAFDIAQPLRLASTAAMSKETGASLVDTRVGTRASIGPLTETDLLGGELSIWIP
                     TGNEDKGTSDGSSRVHIRGIVSGRRDAVDYTASLGWLHRKKADVGNLTIGPAITFGAG
                     ASFGLVRDLVWLGPEIAGHFVLPGDGVDTGWSRSVPVEGSATTRVRVGRWLLGGLLGT
                     GLTPTPGTARFRVELSVATVASITVGPSDWDGDGIEDGRDACPRTPGLANSDPRKHGC
                     PFPDIIPSDRDGDGIADDRDACIETAGVSSDDPWKHGCPPDRDGDGIVDELDACPDTV
                     GEPSEEPSKHGCPRDSDGDGIIDALDACVDVPGVASEDPGRHGCPADRDGDGIVDELD
                     ACPDTPGQAHEDKDKNGCPPVAIVADEIRITETIHFATRSARILPESDALLGQIAELL
                     QDHPEILLVSIEGHTDSRDNAYFNQQLSTWRAASVQKWLVQRGKVAPARLTSVGYGKD
                     RPIASNDTPEGMARNRRVEFHIKKRATVDEEPSPTIR"
     gene            12001..13194
                     /locus_tag="PK710_01585"
     CDS             12001..13194
                     /locus_tag="PK710_01585"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOT08424.1"
                     /translation="MHNSLFRWIPWLLWLVSACGSDEQADGPFHGGGSGGADSGADTG
                     DAVADAREDAKDTGGSAGTGGSAGAGGSAGAGGNAGAGGNAGVGGNAGVAGVGGEAGS
                     SGSAGAAAGGAGGSSGSSGSAGAGANAGAAGSSPTEPVTLRIVAANLSSGNNQSYDPG
                     HGKRILQGLKPDIVLIQEFNYGSNSSADLQNFVDSTFGPGFSYAREAGQIPNGVISRY
                     PIIASGEWDDPEAPNRDFCWARIDLPGPIDLWAISVHLLTSSGSNRVKEANALMAKVQ
                     THVPQGQYVVVGGDFNTGSRNEGCIDKLSARFVTDGPYPVDQSGNGNTNAHRNSPYDW
                     VLASPELDSLAIPTRIASQSFSNGLVFDSRVYTPLSDVPPVLVGDSGASQMQHMAVVR
                     DFEIP"
     gene            13204..14103
                     /locus_tag="PK710_01590"
     CDS             13204..14103
                     /locus_tag="PK710_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOT08425.1"
                     /translation="MPASDAIATIVSVGRLHIVAIAALGTLTFGWLFTGHHPWILALI
                     CATDWFVVNLLNRIVDIKEDSANRIRGTAFVARYRHVVFAFGLLLLFGTMAWTHGWEP
                     HLTPLRLAYHVLGFAYNWPLIPWVGRIKTLYFWKNTASAIGFLITVFGYPLATVSWGL
                     DAALVPSGITPITVVFAAIFFFLFEISYEIIYDLRDAQGDALAGVRTYPVVHGEAVSL
                     RIIDALLITSCVVLCGGYAMGHVPWRMVVMVLAPCLQGVLYKRAYRRGITSGDCIRLT
                     WVGAGLLLGYHLWIFVGLPGVDS"
     gene            14100..14927
                     /locus_tag="PK710_01595"
     CDS             14100..14927
                     /locus_tag="PK710_01595"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HOT08426.1"
                     /translation="MMVLEWVAYAIVATFVVVRSRFGPRPDAFLRRLALLVVASFLVE
                     DTAIHAFGFYQYSPDWSVFVDQVPLAVLLIWPIVIHSSWELARALLGSDHRAVPIAGG
                     ALVCADASLIEPICSRAGLWSWNEPGLFDVPAIGILGWAFYASLCMVAFQWAEIRRAP
                     RWEWACWLLAPAGTHLLLLVAWWALFRWVNTPWPSWPIVGAAWCVSLGLMLHAWKTHA
                     RQRIHPRDLLIRIPAALFFFVLLALRGRDDPALVAYALAFAPPYLALMQNPRAWRWA"
     gene            15164..18586
                     /locus_tag="PK710_01600"
     CDS             15164..18586
                     /locus_tag="PK710_01600"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOT08427.1"
                     /translation="MKRRRHTNPHEPELTIETLSMEGNLLFPEWLSKIAQRQADKQTD
                     ADYRIPQGQRLHHEIDRCWHIAQTLWTTFSADPKKNTITKAELFVSALLRSAFGFVSL
                     APVKPASLKNGLFSISYAAVDGRVPIVVAPAPSGLDTLLDAFGDANRKPCAYGLLQEY
                     LHARNDALWGIASDGARLRIVRHDEGLTRPAWIEADLRRIFVEKREADFAALWLLCHE
                     TRFGPPNRPVAEGILEKWCEAGKQQRLQAREQLRRGAQQALIALGQGFLSHPRNEDLR
                     AALQNGTLSEKSYFNQLLRLVYRFIFLFTAEERGLLHPEKTPEPIKTLYENSYGMGRL
                     RELARKRGAVDHVSDLWATTLAVCRGLAQGEPRLGLPALAGIFATNQCPDLDRASLDN
                     HALLAAVFHLAWRKDDDRLYRIDWHLIDSEQLGSVYESLLELVAQTLPQPPYFSFVTD
                     DDTQGNTRKTTGSYYTPDTLVRVLLDHALEPAIARTIADHPSQPTEALLRLTICDPAC
                     GSGHFLLAAAHRLASHAARRKANGTASPAQYQSALRHVIRQCLFGVDLNPMAVELCKI
                     GLWLETAEPGLPLTFLDSHIQHGNAILGATAERMANGIPDAAWDPVEGDDKRTSRLLK
                     KRNKFAHKEYVSTISPKRASAGSGPQSIRSAATNPGMVDDLWLEHPGKKQEGREATLD
                     SSLIRRQKLVADAWCAAFVWPKQPGQLAEAAPTNDVWLRLRDGLEAASALTEKTVHEL
                     AEQYCIFHWHLQFPQIFKKGGFDVVLGNPPWIAHAGRAAQPLPPGVRRFFETNCEAFA
                     DYPTTHGLFASVIPEMVRIGGHVGLVVPSSMSELQGYAPTRSAHDRLCEFFNEMTDFG
                     EGRFEGVTQPCMALVSRRSEKGRQDAAPGEPWPMARPELSAIDQRLLERLLRLPPMAP
                     ELFGERGVQSDKTLTQHFVRSAQPIGRFTTPIREGTDVREFNLLPPRFHVDSEALGPR
                     IRSAQEFATVRVVLRQTARYPIAALSDGLPFRNSLLAAFESSTWPATAIVALLNSALI
                     RWWHYQRFRDARQPVMPQVKIGHLRAIPMPPSVLASDVSGLADVGRRMSASRGDEGRE
                     QLDSLVADLYGLTHEERATVEQWHQNMGPKGPGSSKLANFKGKPPRRHAKNS"
     gene            complement(18868..19638)
                     /locus_tag="PK710_01605"
     CDS             complement(18868..19638)
                     /locus_tag="PK710_01605"
                     /inference="COORDINATES: protein motif:HMM:NF013214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HOT08428.1"
                     /translation="MQPMTSRPTPYGGSIVADEVQVRLAFLKKVYSLLTVTVLTAALG
                     AFASLYLGAGTSQLAVATASGGEVIVPPLVAFFANHWIIGGILFLGSVFGASFVRHRP
                     GINLAALLGMGFMSGLIAGPAVFVAQLAATSGTALTLNPVRDAFLLAVAGFTGLTFTA
                     LTSKRDFSFLRGFLSIGLWVVLGALILGFFVQSSVFGLAVASGGVLLFGGFVLYDTSR
                     LMRTPADQMDPVGATISLFLNFLNLFLFLLRIFTGSRD"
     gene            complement(19750..>21543)
                     /locus_tag="PK710_01610"
     CDS             complement(19750..>21543)
                     /locus_tag="PK710_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOT08429.1"
                     /translation="QWVGSVLSAVTHHRGLGATTFAVVGGGVVAVCALVAWRLAAGVR
                     SISQRPVVAWTAFVLACVAVGAAVYVATRRESGQPEVAHAVADAGIFLGAAIATSRIR
                     LPSVHKRVWVPITVVFLVLLIAGGISLLHRLAVAPGVRDQCLIVSPALDLIAEPDTRP
                     KRRSKPKNTNEPAMASSTETPTANASSSASTSAPVVAPQTSEKAEKQASAAATLPEKP
                     DIIVVTLDAVRADHLGCYGYERKTSPGLDALAAKAVVFERAYAAGPETRTAMAPLVTC
                     KHLVESVRDSRPWPTLLRANETVAERLRAGGYATAAVSSFQWLSKARGFDQGFDVFDE
                     APFKRVRPEKDMTGAHAVAQAMAAYDQMAAGDKPLFLWVHLFDAHESYLNHESFDFGK
                     RDIDRYDSEIAYLDNELSRLLTHVSQAPRSAKTVWIVHGSHGEAFGEHGFRGHPPKLF
                     EEVLRVPLLIQAPGIKGRRVTDAVVSVLDVPATVLSLATGEAKDCSGVSLVDLVQEKV
                     SEVKERPMLLVAYDGLHGQAPAYGWLDSRVKYVLYAWREGERTRLFDLRADPREQTDL
                     RQERPDQAISLRNQLDTHLQKSLKRLDAVEP"
     assembly_gap    21544..21553
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21554..21863)
                     /locus_tag="PK710_01615"
     CDS             complement(<21554..21863)
                     /locus_tag="PK710_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08430.1"
                     /translation="MADSTWPLPTRDLFAHISGEIALASILTGSGAAVAMSSRVAQQA
                     GFGVAWLSLAGVWAALAVVIIFFLRTATGGLHKAFPDPSSRATLLFAGAIWIILSVAL
                     L"
     gene            complement(22085..23704)
                     /locus_tag="PK710_01620"
     CDS             complement(22085..23704)
                     /locus_tag="PK710_01620"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT08431.1"
                     /translation="MTLRPYQRETVTAVIEARRQGCKRMLVCLPTGSGKTVIFSHLAS
                     LARRPVLVLAHREELLTQARDKIQNALGDHRVVAIERGMDRASADAHVLVCSIRSLHE
                     QRLSRVMQDRHFGLVIYDECHHAAAQDNRRVLSQLGVFEPDWQGTLLGFTATTTRGDG
                     QGLHEIFERIVYTRTLPEMIRDGYLCPLRGFRISTASDLRELSNSGQDFREDELEAVI
                     NVEERNALVARSIQELARDRRTIAFCVTVAHARALCKALNHLGVPSGMVHGQMPSERR
                     ASELASFRQGKTTVLTNVAVLTEGFDDPGVSCVAMARPTRSEGMYAQCVGRGTRLFPG
                     KRDCMILDFVDVSKLRLCTLPSLFGMPYELNLLGEEALGASQRYERIVFDYPGFEWEA
                     GEITLREIQQRAKQFDPLGLQLPGEVRAISNHGWVSLGSRGLALHFEKRAGQITEVLV
                     LARAGHGKRWEVSMDGKVVERFSTVEQAVEAVDYEVRKLGPRASASALAQASWRPQQA
                     PPNAPAWRGRIPKTKEEALRLEAFRKVTRKA"
BASE COUNT         5023 a         7409 c         6342 g         5079 t
ORIGIN      
        1 agtggtaaga cgcagtccac gacgcgtatg cagcatcgga gagtatcgca cttccatgta
       61 gcgaacgttt tcgcgcgccg catcttccgc tagctcgtat gccgctcgga aaagagattc
      121 cttggtctgc agtacactca acgtcacatc gaatgtcttg aggtactcca ccaaagaacc
      181 gtaattggtg ccaaatcgta ataacctgcg cagtccatcc gcatcatccg cgggaagcgt
      241 tacgccctgt tcccgcgcta aatccaaaat ggtgtcgacg cgcatcgaac cgtcgaggtg
      301 aacatggaga tcggtcttgg ggagcttttc gaagaactcg agagggagct tgatatcttg
      361 ggttgtgtcg gccatacggg accctatcac aacttcggac gtcgtcagcc gttgactcat
      421 cgcgtcaatc gcttcatgct gcgcccagtt gaaaaactgg tgaattggcc cccatggctc
      481 cctattcgcg cgggcctagg gggcgaggaa tgtcttttac ctgcacggaa gcacaaggag
      541 acggacccat ggcgactgcc ccgcagaatt tgtacaaggc ggacctgcga gacgtgatgt
      601 tcgttctgtt cgagcaattc aagctcggag agttgctggg caaagaaccc tatgaggcat
      661 ggggaagcga agaagttcgc atggtgatca acgagtgcta tcgcttttgt tgcgaagtgc
      721 tcggacccgt caatgctctt ggcgacacgc aaggatgcac catcgaaaac ggccaggcca
      781 aagtgccccc ctgcttcaag accgcccatg cgcaactcta tgaagcaggc tggaaagccg
      841 tcggagtgag ccctcacttc gatggccagg gggcccccaa ggccgtctat atggcggtgg
      901 aggaattcat cacaggcgcc aatacctcgt tgagcatgta tccggggctc gctcacggcg
      961 cggcggaact catcgaaacc tttggtacgg aagagcaaaa agcgcgctac tgccacaaga
     1021 tttttcacgg cacctggggg gggaccatgt gtctgaccga gccagaagcc ggctcagacg
     1081 tgggtgcggc ccgctccaaa gccttcaaaa atcccgatgg aacctaccga atcgagggaa
     1141 cgaaactgtt catctccagc ggtgactcgg acatggtgga aaatgtcatc catctggtcc
     1201 tcgctcgtat cgacggtgcc gccgctggca ccaaaggact aagcctattt atcgttccga
     1261 aactccgtat caaggaagat ggttcttgcg gtgaacccaa cgatgtcgtg ctcgcgggcc
     1321 tcgaacacaa aatgggcatc aaaggatcaa ccaccgcgct gctgaacttc ggtgagaacg
     1381 gccagtgcat cggtgaactc gtgggcggtg tcgagcacga aggcatgaag cagatgttca
     1441 tgatgatgaa tggcgcgcgc atcttcgtcg gtatccaagg gctcgccaac gcctccagcg
     1501 cctatctcaa tgccctcact ttctgccgcg accgaaaaca aggatcttct atcgataatt
     1561 tcaaggaccc caatgcccct cgtgtaccga ttatccagca tccagatgtc cgacgcatgt
     1621 tgctcgacat gaaatcacgg gtggaaggca ttcgcgcgct cgccatcaag ttgtccatgc
     1681 atcaagattt ggctcgtgtc gcttccgacg aaaaagccaa agaccaccat caggggcaag
     1741 tcgacctgtt gacaccgatc gtgaaagcgt atggcaccga tgaagcctac cgcgtgtgcg
     1801 tgacagcgat gcaggccatg ggaggcgccg gctacatcag cgattatggt gtcgagcaat
     1861 attgccgaga ctcgcgcatc ttcaccatct acgagggaac caaccacatc caagccatgg
     1921 acttggtcgg acggaaactg agtcagcgaa aaggtgccaa ttttcaagag cttgtgggag
     1981 atatcgccaa gttcgtggcg aaacataaag agcatccccg gctcggcgca ccggtccaac
     2041 tcctcgccaa agcacaagaa accatcgctg ctacagccat gctcttgttc aaatggtcac
     2101 agtccggaaa actgaaatat gttgatctgc acgctaaccg attccttgaa atgatgggcg
     2161 ccaccaccgt ggcatggctg ctgttggaag ccgccctcat cgcggaagaa gccttgcaaa
     2221 acgtgcaaaa cgatcatccc gatcgcttct tttacgaagg aaaaattcag gccgcccggt
     2281 tttttgccca caataacctg cccctcgttc ctgccctcgg cgccatgatg caaacggacg
     2341 acgattcggc gatggccatt ccggaagaag ctttctccac gatctgaatc tcaccccctg
     2401 cgcatgagca tcgagactcc ccgctccgtg ctagcatccg agcatgcaca ccattcgact
     2461 ctccacgctt tcccttgccg cttgctgctt ttcgcttcca gcatccgctc aatcatcacc
     2521 gcctgcccat gatgattttc aatcgggtgg gctggctccc cctaccgctc cccctcccat
     2581 ccaaacgcaa catcctggat ttcaaagtga aaccgagaga accctcgatg aatccgaaaa
     2641 agaagatgca ggaagaaacc tcgattgggt ctacttcgat gtggaaggcg gctacgaagt
     2701 cgtggggttg gaaacattga aaagcaaagg tctgacctac gatgatgcgc aaacttcgga
     2761 cggcgggttg atgctgggaa cgggtcttgg actgcgtttg atctacatga ccgtgggtgc
     2821 tcgcgcccgc gtcggaatgc tttcgctcgg taacctcgga accatcaacg gagaatttgg
     2881 ggtcaatttg cccctcggcg acctcgaacc gtccttccac gtcggtgccg gttatgcctt
     2941 cttggcttct cccgatgccg atcgttgggg cggcgacccc acgatccatg gttggaacac
     3001 ccggctcggc gcaggcttcg attattatgt gacacccgcg ttttccgtag gcgcgcggtt
     3061 atccggcgat gtccttttcc tgtctcgaaa agccatgacc ctagagccaa cctcgaatct
     3121 cggtgcaaac gcacgacaag ccgcttcctc caaaggcaac gggatcggcg ccgctttctc
     3181 cgcttccctc ggcttggctc tgcacttatg acgtctcgcg cgaagtggat atgacagctt
     3241 ggatcgcccc tgcatccgtg tggctcatcc tatgcgctat cccttgcgta gcatggctgt
     3301 acttcctctg gcgcctgccc cctcatcgca cgtctgcatt cgccgcatgg ggcaccgcgg
     3361 tattgggcgc cttcatgggc gtcgcttcct cctggattca acgattcgtc gtcgaactta
     3421 ccgggttgga acaagccgtc tccacgttcg gcgctcccag cgggttgctg tattttctcg
     3481 tcttcgcggc tcccttggcc gaaggcgcca aagtcttggc agcgtggccg gccttgcgct
     3541 ccgctcatgt cgatgagcga tacgacggtt ttctcttcgc ttcggcctcg tcctgcggct
     3601 tcgcggcaag ccaaagcgcg gtcgcgctgc tcagtgcccg catcgatatc gacgtcatgt
     3661 tgcgggtcat gctgctactc atcgctcatc cactgatgtc ttcgttctgg ggacacgccc
     3721 ttggaaaaat gcggcgcttc cgtattccta ccggcagctt catcctttct tggacactcg
     3781 ccacgctcgt acacggtctg ttgttgcact tgactcgatc cacctcatgg ttcgctttgg
     3841 tagccgcttt tcccgtgctt ggaggccttg cctttgccac cgggtgggca gcgcgagatc
     3901 tgctcgctcg atttgcggtt tccgcacgcc cctccaggca aagcatgctt tccctgttgc
     3961 cgtctccctc gttgcatgcc gttcgacacg cccttcgaag aaccgatcga cccatcatgg
     4021 tgcattggat cgtggttggc gcgctgacca caaccggagt catgttaacc atggtggtct
     4081 tggcggtgtg gttcggtcat caagtggggt tggatttttc cgccatcgaa cgggaagaca
     4141 ccaccgctcg cgccatcgtt cccttggtgt tgatcaccgt ttccatcctc gccgcattcc
     4201 ccgtgtcagg ctacttaatc acgaaagcaa gtggttcgga taacgttctc gaacccgctc
     4261 tctccgcgac cttggccatt gccgctgtgc tcatcatgct cggcatcgcg gctcctgttg
     4321 cactcgtatt tgctctcgcc ttcgcgccta tcgccttcgg actcgcctgc tcgggagctt
     4381 ggttcgggct cgataaatga cgttcaccga ctcgcttgca gcctttgctt caaacgtagc
     4441 caggcacgac gctcctgttc attggtcaat gaatcgatga cttgctccac ccggctgagc
     4501 gtgtccggat gctctaccat cgacaacgaa cgtgcgattg gctcgcgtaa tgcctcccca
     4561 cgtcgcgaaa acgcatcgag aagagcctct gcacacttgc cgcggattgg catctcgtac
     4621 tcttttttac ctgctcgacc ggttcgccac gcccaggaag aacccatgta ccccaaagca
     4681 cgcgccgcca gcttcgccgt tggttccgat gagtccatga gccagggaac cacagcgtcg
     4741 accgctctga gatcacgtac gtaacgcagc ccccataacg ccgcgtctct tctctgtcct
     4801 ttgcgctgca atgcattgat taacaacgtt ttttcactct cgccccccaa ccttcccaaa
     4861 ccgattgccg cccctcgtaa cgcaatccaa tccgaattct cgtgcgtaaa tatcgcgcga
     4921 aataccggac tcgcttccgg catttcccgt tctccgaggg cgatgatgat cccttcccgt
     4981 acggcatccc actctcgttg ggtggcctct ccccgctccg gtgctcgaaa ggataccgct
     5041 tccagcaagg cccatcccgc cccaggtccc aacgattgaa gctcagcgag aacgctcggc
     5101 tgctgagatc gaaactgtcg atacacctgc ggacgatgac cttgcagtgt ctccaagcgc
     5161 tgaaatgata ctggatccag cgttttgtaa cgggctacct gctgttgtat cgcctctcga
     5221 tcgttgcgag acaaacgagt cgacggcatg aacaagccat cggcccaggc cgctcgcgat
     5281 agcatcagca aggtcagacc gactccaagg attgggaata tggcttgcgc tctcatcgtc
     5341 cgctctccca ccacggatgt tgatggggca ccacccgaag cacattcgtg gcgcaatgca
     5401 cctcgccgtt atttcgatga tatgtgttcc aatcatccac gtagtgggtc acaagccccg
     5461 tcgcgctcaa tcgggtgtcc agatcctgtt tgaatacatc cactcccccc acattcggac
     5521 caaaaggatc ggcgatgagc acatgaccat cgaagtgcaa taaattcacg gttcccggct
     5581 gataggccac cgatccttcc ggaatcgatt gctccagaaa tggcatatcg atgatctcgt
     5641 cgtcttgtag atcgagttcg tctttgagct gcagcaaaat gccatccagg atcgcttgat
     5701 cctcctggtt ggtcgccatc aagttcgcat tgccgagcac ttgatccacc gtccgggacg
     5761 ccggccctcc ccaccaccac ttgccttcat gcattttggc cgatccatgt cccgcttttt
     5821 gcaaggacat caacatatcc accgccatct ttgggcgcgc gaacagcatt cgccagccgc
     5881 gctcgctatt cgcaggaacg aaagacagaa tctcgtccac gtgacccacg gcaagccagg
     5941 aagtatccac ccgaatcaac ggctgcaccc gctgtgcttg cacaaactcc tccgccacgg
     6001 gatcgggatg acgctccgcc gtggaaccta gaatgaatct tccctgggga tacgacaccc
     6061 catgcgctcg atggggggga accgtatccc aattccctgt ggaatccaga gaacgcgtcg
     6121 cttggtcata cggctgatcg tgaacgtgaa tcgcgccgaa atccttgcca agaaactcga
     6181 caaacaccac gtccgctgac gtgcgcttca cccgcggcgt gcgaaacgcc accaacatcg
     6241 tatgcatccc ttccttcgac ggcatcgatg catatcccat ctcgaaatga tcttgcgtcc
     6301 attgatcgaa atcatggttc gcatatcccg gcgcatcgta gttcagcaca tcgaccgcca
     6361 cggcggctgc cgtcgcggcc gctttcaaac ccttgatgaa ttccccatca aaaccaccta
     6421 cccaaatctt ctctgttttc atcaggttat gcatgaatac caggggtgcg acgcgcatgc
     6481 gaacctggtc tctgctcaac tcttcgtgcc cgttggtgac gatatgttcg atgtccacga
     6541 atccagacca cgcccccgcc gctgtcgaag tcgggaaaaa tttcgcctca atggcaaact
     6601 caactccgtg agcaagatcg tccgccgtca caaccacatg cgtgggatcc tcgagaacaa
     6661 actcatctgc ttgaacacga aagagacgaa cccaaggggc cgcttccgga tcgaccgata
     6721 tgatcccctg cgcattggca ggtacgtcct caaatcccac aacgtggatg cgcgccaggt
     6781 ccttcgcatc ctcaggaccg ttgaccacct catcaaggtg atcgcccacc ccgtctccat
     6841 cgtcatcatc cacattcgcc aacaaaatcg cgccgattga ctcgttccat tgatcctcga
     6901 acgtctgctc ctcaaacgag tcgggctcaa tcaccccatt gcggttggca tcgaccacga
     6961 gatcgccaag aaccctacga gtcacgggtt catcgctgtc gccttgccca tcctgcccat
     7021 cgatcccttg aacgtcttgg gtcccctcat ccttgtcctc agcgtcatgg cctccagcat
     7081 cgcttacagg ggccgaaggc ttcatcaagt ccatggtctc ttcggaactt gaacagccct
     7141 gggccagtat caaccccaat cccaaggcta tcgacccatg gaaccgcaat gggcttttcg
     7201 gttcgaacat agacagcaca cgaagcatcg taagacgttc tagcaccaag ctgacgtcat
     7261 gtcctgatgc gatcttcggg ggaacaatgc cttttgtcga tggacacggt cactcgagcg
     7321 gtggtgtttt ctctgaacac gcattccgtg ctaggcaacg gacccatgga cgcgcgaggt
     7381 cgatacgacg cccgaattcg aacgatcgaa cagcagacga atacgctgca gcgacgatat
     7441 gacctattgg cctggtcccg gctttccgtg ttcatcctcg ccatggcttc cgcgatcggc
     7501 acgctttgga aagatctcgg ttcttccggt tggtccgtga cgctcggtgc ttccgtgctc
     7561 tttgccgtac tcgtttcgta tcacgctcgc atcgcccagc aacgcgatcg gttacaggcg
     7621 gcgcgacgtc acaacgaacg aggaatcgat cgtctcgatg ggaaatgggt ggatttcgaa
     7681 gatgatgggg ctcgttatct ccgcgatgag catgcgtatg ctttggacct tgatattttc
     7741 ggcaaggctt ctttgtttca actcttgaac gatacggcca cacggatggg cgcgcgacgg
     7801 cttgccacgt ggctggcggg tccggcgcaa gacgacacac tacggattcg acaacaggca
     7861 gtgcaggaat tggcgacgtt gcactcgtgg cgtgaaagcc ttgccgtggc gggcgcgcat
     7921 gccgacgata ccttgccgga tcccgaaccg ttgttggttt ggatggagcg ttcccccacg
     7981 ttcaaaactc caacgattcc gcgtatttgt gcgtggattg ggacgtgttt ggtcttgggg
     8041 gcggcggtgt tgtgctccgt gctttctctt tccgcatggg tgtttctcct gccgtacttg
     8101 gtgaacatcc tagtttcgat agcgttgtca cgaagactgc agcccatcat cgacgctgta
     8161 tccgaacgcg ctagcgatct tgaacgtcac ggcgagatgt tcgagttgat ggaacgacaa
     8221 gcctttcgtt cggaaggtct tgttgccctt cagcagaaac tcaaagcctc cggccgcttg
     8281 gcttccgcgg agacacgaca gcttgcgcgg atcgtgtcgt tcttggacgc gaggagaaat
     8341 ggcgcttttc gagcgctgat tggatcggcg ctgctctggg atcttcattg cgttttcgcg
     8401 ttggaacgtt ggcagaggag ggtgggacct tccgctcggg gatggatgcg tgtgcttgcg
     8461 gatctggaag cgctgtcttg ctttgcgaca ttcagcttcg agcatcccga tttcgcgttt
     8521 ccgtcgatgg atccggggga actgcatttc gaggcgcagg ggctaggaca cccgctgatt
     8581 agcgatgacg agcgggtcgt caacgatgta tcgctgcctg ggcccggctc cctgttgctg
     8641 gttacgggtt ccaatatgtc cggaaaaagc acgttgttgc gctcgattgg cgtagccgcg
     8701 gtgcttggct tggctggcgc gccggtttgt gctcgctgtc tgcgaatgtc cgcatgtacc
     8761 gtcatgtcaa gcatgcgcgt gagtgattcg ctcaaagacg gtatctcgcg gttttacgcg
     8821 gagttgatga agatcaaacg ggtgaccgaa acggcagaga aaactccggt gctgtttttg
     8881 ctcgacgaaa tcttacatgg aacgaattcc cgcgaaagac acgtcggcgc ccgatccatt
     8941 ctccgaatgc tcatcgacaa cggtgccatg ggcgcggttt cgacccatga tttggcactc
     9001 gcatcgcttg ccgatgaact ccccgaccgc gtgcggctcg tgcatctgca agaacaagtg
     9061 gtggatgata aaatgatttt tgactatctc ttgcgagatg gagtcgtgaa aagcgggaac
     9121 gcgctacgct ggatgcggtt cgtcggactg agagtggatg aaccctgaag gcagaagaaa
     9181 aagtccgtcc tccggtcaac ccagagacgc tagaacgagt agccgatcgt gaatagcaac
     9241 cgaggaatcg taccgctgaa cttcacggtg gaactcgtgt tactcacgct cgatgatgtc
     9301 ttcaagtaca tggcaccacc acccacgccg atggtgaatc cgctgtttgc cacatattga
     9361 tacccaaggt cgatcgccac accgtaagcc gaaaacgaag cttctgcatc gtcaaggaca
     9421 caaccattgg atttgcattc gtccttcgct ttcgaagtca gaaacacgaa ctgggggccc
     9481 acaaagaaac catctgcacc gcaatcccca agataaaaat gataccccaa ctccgcaccg
     9541 aaagacgtat acgtcgtctt gatgtccgag ccttcggcac tggccgacga aaaataggga
     9601 ttcaacatga tgccgtgatg gggcgcggga agatactcaa cgttgagtcc aaatcggcca
     9661 agaattaggg tgagcgggtt gagcgtcagg gcgacaggtt tgaagtcttc ttgaaccgat
     9721 gaagccttgg cttcctcttg cccttcttca gcacttgcac tccaggttgt cgtcgaaatg
     9781 gcaagtgacg tcgccacaca acaaacgatt ccaactccac gttttttcat gtgatacttc
     9841 tctttcgcta ggttgatgcc cgatcgaata agatgaacga aaacgtctac gctctcttgg
     9901 ccaaggaaag cgatcagccc gcgccgagct tactacggag cctttcgact gagtcctccg
     9961 tctgcccaac gctttcacac ctatgggtcg tcctacgtcg accccccaca actcattccc
    10021 tgacacgaga cgaatttttg tattgttttc accacgatcg cgcgtttggt gcaaatcccg
    10081 tcagaacatc atccatcgcc catcaacgga tggtgggtga aggctcctca tccaccgttg
    10141 cacgcttttt gatatgaaac tcgaccctac gattacgagc cattccctcc ggtgtgtcat
    10201 tgcttgcaat aggtcggtct tttccatatc ccacgctcgt caaccgtgcg ggcgccactt
    10261 tgcctcgctg caccaaccac ttctgcaccg acgcggctcg ccacgtggat agttgctggt
    10321 tgaagtaagc gttgtctcga ctatccgtat gcccctcgat cgatacgagc aatatctctg
    10381 gatggtcctg taacaattct gcgatctgcc cgagcaacgc atcggactcg ggaaggatgc
    10441 gcgcgctgcg cgtcgcaaag tgaatcgttt ccgtaatccg aatctcgtcc gccacgatcg
    10501 ccaccggagg gcatccgttc ttatccttgt cttcatgcgc ttgcccaggc gtgtccggac
    10561 acgcatccag ctcatccacg ataccatccc catcccgatc cgcgggacaa ccatgtcgac
    10621 caggatcctc gctagccacc ccgggcacgt ctacgcacgc gtccaaagca tcgataatgc
    10681 catccccatc cgaatcgcga gggcagccat gcttcgacgg ctcttcgctt ggttcgccaa
    10741 ccgtatccgg acacgcatcc agctcatcca cgataccatc cccatcccga tccggtggac
    10801 atccgtgttt ccaagggtca tcgctggaaa ctcccgcagt ttcgatacac gcatcccgat
    10861 catcggcaat cccatcgcca tctcgatccg aggggataat atccggaaat ggacacccat
    10921 gttttctggg atccgaattg gccaatccag gggttcgggg acatgcgtct cggccatcct
    10981 cgatcccatc cccatcccaa tccgaaggcc ccacggtgat ggaagccacc gtggccacgc
    11041 tcagttcgac gcggaatctt gcggtcccag gcgttggtgt caaacccgta ccgagcagcc
    11101 cgcctagcaa ccaccgtccg acacgcactc gcgtggtggc cgacccctcc acgggcactg
    11161 acctcgacca tcccgtgtcg accccgtcgc ccggcagcac gaaatgaccc gctatttccg
    11221 gacccagcca caccaaatca cgcacgagac caaaggaggc tccagcacca aaggtgatgg
    11281 cgggaccgat cgtcaaattc cctacgtcgg ctttttttcg atgcagccac ccaagcgagg
    11341 ccgtgtaatc caccgcatcc cgtcggccac tgacaattcc gcgaatatga acgcgactgc
    11401 tgccatcgct ggtcccctta tcttcatttc ctgttggaat ccaaatactt agctctcctc
    11461 cgagaagatc cgtttctgtc agcggtccaa tcgaagccct ggttcccacc cgcgtgtcga
    11521 cgaggctagc gccggtttct ttcgacatcg ccgcggtaga cgcgagtcga aggggttggg
    11581 cgatatcgaa agcgagccga ttccacaacg cgtacgacgc cccaaggagt gtgaatattt
    11641 ccgctccctc ccacagacct ttttcttctc gctcatcgaa gacgcttcgc ggccgatagg
    11701 cgtagtccca ggaccagcca acccagggtt caccatctcc ccgaaccatg gcatctggaa
    11761 cgagacgaaa tcggtcgccg gggctcgtgg gctcgaaaat ccgtgtcgaa gcacccggtt
    11821 gggcggacac gggtgcagac accaacccca cggcaaccaa ccatgcaata gcccgccgtt
    11881 tggaaaacgc cgaggaccga atcaagggat tcatgacaat ggtgaaacct accatgacct
    11941 tacgccgcat cggtaagaac gccttgacga tccctgctcc tgcggcaacg gttgaaggac
    12001 atgcacaata gtctttttcg ttggattccg tggttgttgt ggttggtcag cgcgtgtgga
    12061 agtgatgaac aggccgatgg gccctttcac ggtggtggaa gcggtggcgc ggattcgggc
    12121 gccgataccg gggatgcggt agccgatgcg cgggaggatg ccaaggacac gggcggttct
    12181 gcaggcaccg gcggaagcgc aggcgctggg ggaagcgcag gtgctggcgg aaacgcgggt
    12241 gctggcggaa acgctggcgt tggcggcaat gcaggcgtgg ccggtgtcgg aggcgaagct
    12301 ggttctagcg gctccgcagg ggccgcggcg ggtggagcag gcggttcgag tggctcgtca
    12361 ggctccgccg gtgccggcgc caacgctggt gccgctggca gttcccccac cgaacccgtc
    12421 accctgagaa tcgtcgcggc aaacctatcg agtggaaaca accaatccta tgaccctgga
    12481 cacggaaaac gcatcctcca aggcctcaaa cccgatatcg tcttgattca ggagttcaac
    12541 tacggctcca attcctcggc cgatctgcaa aacttcgtcg attctacctt tggacccggg
    12601 ttttcctacg ctcgggaagc aggacaaatc cccaacggcg tcatcagccg ctaccccatc
    12661 atcgcttccg gcgaatggga tgaccccgaa gcacccaatc gagacttctg ctgggctcgt
    12721 atcgacctgc ccggtccgat cgacttatgg gccatcagtg tccacctgct caccagttcg
    12781 ggttcaaatc gtgtgaaaga agccaacgcc ctcatggcaa aggtgcaaac ccatgttcca
    12841 cagggacaat acgtcgtcgt aggcggcgac ttcaacacgg gaagccgtaa tgaaggttgc
    12901 atcgataaat tatcggcgcg attcgttacc gatggccctt accccgtgga tcaaagcggc
    12961 aacggcaaca ccaatgccca ccgaaacagt ccatacgatt gggtgttggc cagtccggaa
    13021 ctggattccc tagctatccc aacacggata gcctcacaaa gcttttcgaa tggtctggtt
    13081 ttcgatagcc gcgtgtacac gcccctgagc gatgttcccc cggttctcgt aggtgattcg
    13141 ggcgcttccc aaatgcagca catggccgtg gtgcgggact tcgagatccc ctaagctctc
    13201 gccatgcctg cctcggacgc gatcgccacc atcgtgagcg tcggacgcct acacatcgtt
    13261 gccatcgcgg cgttgggtac gctcaccttc ggatggttgt ttaccggtca tcatccgtgg
    13321 attctcgcgc tgatctgcgc cacggattgg ttcgtcgtca acctgctcaa ccgtatcgtc
    13381 gatatcaaag aagacagtgc caatcgtatc cgaggcacgg cgtttgttgc tcgctaccgg
    13441 catgtcgtgt ttgcctttgg tttgcttctt cttttcggaa cgatggcgtg gacccacggg
    13501 tgggaacccc atctgacacc cttgcgcctt gcctatcatg tcctgggatt tgcctataac
    13561 tggcccctca tcccttgggt tgggcgtatc aagaccttgt atttttggaa aaatacagcc
    13621 tccgcgatcg ggtttctgat aaccgtattc ggatacccac tggcgaccgt ttcatgggga
    13681 ctcgatgccg ccctggttcc ttccggaatc actcccatca cagtggtatt cgccgcgatt
    13741 ttctttttcc tgttcgaaat cagttatgaa atcatctacg accttcgtga cgcgcaaggc
    13801 gatgcccttg ccggagtacg aacctacccc gtcgttcacg gagaagcagt ctctttgcgg
    13861 atcatcgatg ccttgttgat cacgtcctgc gtcgtgctgt gcggaggcta cgccatgggt
    13921 catgtcccct ggcgcatggt ggtcatggtg ttggcacctt gtcttcaagg agtgctgtac
    13981 aagcgcgcct atcgacgcgg catcacgtcc ggcgattgca ttcggctaac ctgggtcggc
    14041 gctgggttgc tgctcggtta tcatctgtgg attttcgtcg gacttcccgg agtcgattca
    14101 tgatggtgct cgaatgggta gcctacgcta tcgtcgcaac gttcgtggtg gtccgaagcc
    14161 gcttcggtcc gcgcccggat gcctttctcc gacgtttggc tttgctcgtg gtcgcaagct
    14221 ttttggtcga agacaccgcg attcacgcct tcggattcta tcaatactca cccgattgga
    14281 gcgtgttcgt cgaccaggtt cctctggcgg ttctgttgat ctggcccatt gtgattcatt
    14341 cgtcgtggga actggcgcgc gcgctgcttg gttccgatca tcgtgccgtt cccatcgcag
    14401 gcggagcgct cgtttgcgcc gatgcatcgc tgatcgaacc catctgttcc cgcgcgggct
    14461 tgtggtcctg gaacgaaccc ggtctgttcg atgtgcccgc cattggcatc ttgggatggg
    14521 cattttacgc ctccttatgc atggtcgcgt ttcaatgggc ggagattcgc cgtgccccac
    14581 gttgggaatg ggcttgctgg ttgttggctc ccgcgggaac ccatctgttg ctgctggtgg
    14641 cgtggtgggc actgtttcgc tgggtgaaca ccccgtggcc ttcttggccc atcgtgggag
    14701 ccgcatggtg cgtttcgctg ggattgatgc ttcatgcgtg gaaaacgcac gcaagacagc
    14761 gcattcatcc acgcgatttg ctgatacgta tcccagccgc actgtttttt ttcgtgcttc
    14821 tcgccctgcg gggacgtgac gatcccgctc tcgttgccta cgccctggct tttgcccccc
    14881 cttacctggc tttgatgcaa aatccccgcg catggcgatg ggcttgattc gtttcagccc
    14941 cgatcttgcg ccctttttcg gcatcgtgtt tccaccacat ccggcaagcc caaaagagcg
    15001 cttgtgttat cgatggcccg cttgaccaca cgaacgtaac gaactcggcc tctttgtgat
    15061 aaccatccaa gacccaaagg aaaaaggcca tggtgccatc ccatgaccca gccgacataa
    15121 tcgccgaaat cgccaatccc gtcccatctg tccggagccc agtgtgaagc gccgccgcca
    15181 caccaacccc cacgagcccg aactcaccat cgaaaccctc tcgatggaag gcaacttgct
    15241 ttttccagaa tggctttcga aaattgccca acgtcaggcc gataagcaga ccgatgcgga
    15301 ttatcgcatc ccccaagggc aacgtcttca ccacgaaatc gaccgctgct ggcacatcgc
    15361 ccaaacgctt tggacaacct tttccgcaga ccccaagaaa aacaccatca ccaaggctga
    15421 actcttcgta agcgccttgc tccgcagcgc ctttgggttc gtatccctcg cgcccgtcaa
    15481 acccgcctca ttaaaaaacg gtttattttc aatatcttat gccgccgtcg acggacgcgt
    15541 tcccatcgtc gtcgcccccg ctcccagcgg attagacacg ttgctcgacg cctttggcga
    15601 tgccaaccga aaaccttgcg cttacggtct cctacaagaa taccttcacg cgcggaacga
    15661 cgccctatgg ggcatcgcca gcgatggtgc aaggttgcgc atcgtgcgtc atgatgaggg
    15721 tctaacgcgt cccgcgtgga tcgaagcaga ccttcgacgc atattcgtcg aaaagcgcga
    15781 ggccgatttc gcggctttat ggctgctttg ccatgaaaca cgctttggtc cacccaatcg
    15841 gcccgtcgcc gagggtatcc ttgaaaaatg gtgcgaggca gggaaacaac aacgcttgca
    15901 agctcgcgag cagttgcgcc gcggcgctca gcaggccctg attgccctcg gccagggctt
    15961 cctttcccat ccacggaacg aggatttgcg cgcggcactg caaaacggca ccctttccga
    16021 aaaaagctat ttcaaccaac tcctgcgcct tgtgtaccga ttcatcttcc ttttcaccgc
    16081 cgaagaacga gggctcttgc acccggaaaa aacgcctgag cccataaaaa ccctgtacga
    16141 aaatagctac ggcatgggtc gcctgcgtga actcgcgcga aaacgcggtg ccgtggacca
    16201 cgtttccgac ctttgggcca caacccttgc cgtttgtcgc ggactcgcgc agggagagcc
    16261 gcgcctcggt cttcccgccc tcgccgggat cttcgccacc aatcaatgcc ccgacctcga
    16321 ccgcgccagc cttgacaacc atgccctgct tgccgccgtg ttccacctcg cctggcgaaa
    16381 agacgacgat cgcctctacc gcatcgactg gcacctcatc gactcggaac aactgggttc
    16441 agtctacgaa agcctgctcg aactcgttgc gcaaaccctg ccacaaccac cctatttctc
    16501 tttcgtcacc gacgacgata cccaaggcaa tacgcgcaaa accacgggaa gctactatac
    16561 gcccgacacc ctcgtgcggg tgctgctcga tcatgccctc gaacccgcca tcgcccgcac
    16621 catcgccgac catcccagcc aacccaccga ggcattgctg cgtctgacta tctgcgatcc
    16681 cgcgtgcgga tcaggccatt tcctgcttgc cgccgcccat cgactggcat cccatgccgc
    16741 tcgtcgaaag gcaaacggca ctgccagccc cgcgcaatac caatccgccc tgcggcacgt
    16801 catccgccaa tgccttttcg gcgtcgacct caacccaatg gccgtggaac tatgcaaaat
    16861 cggcctctgg ctggaaaccg ccgaacctgg cttgccgctt actttcctcg attcccatat
    16921 ccaacacggc aacgcaatcc tgggtgccac cgccgaacga atggccaacg gcattcccga
    16981 cgccgcgtgg gatccggtgg agggcgacga caaaagaacg tctcgtttgc taaaaaaacg
    17041 taataaattc gcacataaag aatatgtttc taccatctcc cccaagcgcg cttccgcggg
    17101 ttctggcccg caatcgatcc gaagcgccgc gaccaacccc ggcatggtgg acgatctatg
    17161 gctcgaacac cccgggaaaa aacaagaagg ccgggaagct accctcgatt cgtcactgat
    17221 tcgccgccaa aaactcgtcg ccgatgcctg gtgcgccgct ttcgtctggc ccaaacagcc
    17281 ggggcaactc gccgaggccg cacctaccaa cgacgtttgg ctacgacttc gcgacggact
    17341 ggaagcggcg tccgccctta ccgaaaaaac cgtacacgaa ctggcggagc aatattgtat
    17401 ttttcattgg caccttcaat ttccgcaaat tttcaaaaaa ggcggcttcg atgtggtcct
    17461 cggcaatcct ccgtggattg ctcatgcggg gcgcgccgcc cagccattgc cccccggcgt
    17521 caggcgcttt tttgaaacca attgcgaagc cttcgccgat taccccacca cccacgggct
    17581 ttttgccagc gtcatcccgg agatggtacg aataggcggc catgtcggcc tggtggtgcc
    17641 gtcgtcgatg tccgaactgc aaggatatgc tccaacccgc tcggcgcacg accggctatg
    17701 cgaattcttc aatgaaatga cggattttgg ggaagggcgc ttcgaaggag tcacgcagcc
    17761 ttgtatggcg ctcgtgtcgc gccgcagcga gaagggacgg caagacgccg cgccggggga
    17821 gccctggccg atggctcggc ctgaattgag cgcgatcgac caacgacttc tcgagcgact
    17881 gctgcggctg ccgcccatgg ctccagagct ttttggcgaa cggggcgtcc agtcggataa
    17941 aacgctgacc cagcatttcg tccgatccgc gcaacccata ggtcggttca cgacgccgat
    18001 tcgcgaaggc accgatgtcc gcgagttcaa cctattgccg ccgcgatttc atgtcgactc
    18061 cgaagcgtta ggccctcgga ttcgctcagc ccaggaattt gccaccgttc gcgtggtcct
    18121 tcgccaaacc gctcgatatc cgattgcggc gctttccgat ggcctgccct ttcgtaattc
    18181 cttgctagct gctttcgaat cctccacgtg gcccgctacc gccatcgtgg cgctcttgaa
    18241 ttccgcgttg attcgatggt ggcactacca acgattccgc gatgcccggc aaccggtcat
    18301 gccccaagta aaaataggac atcttcgcgc gatcccgatg cctccctccg tcctggcatc
    18361 ggatgtatcg ggcttggccg atgtcggaag gcgaatgagc gcatcgaggg gcgatgaggg
    18421 aagagagcag ctcgactcgc tcgtagccga cctgtatggt ctgactcacg aagagcgagc
    18481 caccgtggag caatggcacc agaacatggg tcccaaaggc cctggcagct ccaagctagc
    18541 gaatttcaaa ggaaaacctc ctcgacgcca cgccaaaaat agctgacgat acgcgagcgt
    18601 agcccgctcc aatctctctt tttcgccgtc attgagcatg ccatcagcgt atgccgggag
    18661 caccggatcg atacgttttt cttgaaagcg gtgacgtcga gccagcgccc ggcaccgaac
    18721 caccgattgc tttgccgctg tctgtcgaag acccggtctt ttcacgcgat aaggattcag
    18781 gcaggcgtcc tttcgcgctc aacccacggt tcgcatgttc ggactgtcac aacggatttg
    18841 tggggaaggc ggggaatgga atgtggttca gtcccttgaa cccgtgaaga tccgcaacaa
    18901 gaacaaaaac aggttcaaga agttcaaaaa cagcgagatc gtggcgccta cgggatccat
    18961 ctgatcggca ggggtacgca tcaatcgcga ggtatcgtaa agaacgaagc cgccgaaaag
    19021 cagcacgccc ccgctcgcca ccgctaggcc aaagacgctc gattgcacaa agaatcccag
    19081 gatcaaagcg cccaaaacga cccacagacc aatcgacaga aacccgcgaa gaaatgaaaa
    19141 atcccgtttg ctggtcaacg cggtgaacgt cagccccgta aacccggcca ccgctaacag
    19201 gaaagcgtca cgaaccgggt tcaacgtcag tgccgtgcca cttgtcgccg caagctgcgc
    19261 cacgaaaacc gcggggcccg cgatcaagcc gctcatgaaa cccattccca atagcgccgc
    19321 caggttgatg cctgggcgat ggcgcacgaa cgatgctcca aacaccgagc ccaaaaacaa
    19381 aatacctcct ataatccaat ggttggcaaa aaatgccacc agaggaggca cgatgacctc
    19441 tcctcccgaa gccgtggcca ccgcgagctg agacgttcca gccccaaggt ataacgatgc
    19501 aaaagcaccc agcgccgccg tcaacaccgt gacggtcagc aaactgtaaa ccttcttcaa
    19561 aaacgcaagt cgaacttgca cttcgtcggc gacgatggaa ccgccataag gcgtgggacg
    19621 agatgtcatc ggttgcatga gattttcctc attgggtcca aggatagctg agccggttac
    19681 ccgcgttgga gcttatagct cataaccgct gttttgcctc agtcaacagg cggtttgcca
    19741 aggttgcgct cagggttcca ccgcatcgag acgtttcaga ctcttctgca gatgagtatc
    19801 cagttgattg cgaagcgaaa tcgcttgatc cggacgctct tgccgcaaat ccgtctgctc
    19861 ccgtgggtcc gcccgcaaat cgaataaccg cgttctttcc ccttcccgcc atgcatacag
    19921 cacgtacttc actcgactgt cgagccaacc gtaggccggc gcttggccgt gaagaccgtc
    19981 gtaagccaca aggagcatcg gacgctcttt tacctccgac accttttcct ggaccaggtc
    20041 caccaacgac acgccagaac agtccttggc ctcgcccgtt gccaacgaaa gcaccgtcgc
    20101 gggaacatcc agcacgctga cgaccgcgtc cgtaacgcgt cgacccttga tccctggcgc
    20161 ttgaataagc aacggcacgc gaaggacctc ttcgaatagc ttggggggat ggccccgaaa
    20221 tccgtgctct ccaaacgctt cgccgtgaga accatgcacg atccacacgg ttttggcgga
    20281 acggggggcc tggctcacat gcgtgagcaa acgcgacagc tcgttgtcta aataggcgat
    20341 ttcactgtca taacggtcga tgtcccgctt tccgaaatca aacgactcat ggttgaggta
    20401 cgattcatgg gcgtcgaata agtgaaccca taaaaataag ggtttgtctc cggcggccat
    20461 ctggtcatag gccgccatcg cttgcgctac cgcatgcgct ccggtcatat ccttttcggg
    20521 acgaacgcgt ttgaaagggg cctcgtcgaa aacgtcgaaa ccttggtcga agccccgggc
    20581 tttcgaaagc cattggaacg aagatacggc ggctgtcgca tatccccccg cacgcaatcg
    20641 ctcggccacg gtttcattcg cccgcaacaa ggtgggccag gggcgactgt cgcgcacgct
    20701 ttcgacaagg tgcttgcacg tcacgagcgg agccatggcc gtgcgtgtct ccggccccgc
    20761 ggcataagcg cgttcgaaaa ccacggcctt ggccgctagc gcatcgagcc ccggactggt
    20821 ctttcgctca tagccgtaac aacccagatg gtctgcccgt accgcgtcga gggtcaccac
    20881 aatgatgtcg ggtttttcgg ggagtgttgc cgcggcagaa gcttgttttt ctgccttttc
    20941 cgatgtttgg ggggcaacaa ccggagcgga tgtcgaagcc gacgacgatg cgttggcggt
    21001 gggcgtttcg gtcgaagacg ccatggcggg ttcgttcgtg tttttgggct tcgagcgccg
    21061 tttgggtcgg gtatcgggtt cggcgatgag gtccagcgct ggggatacga tcaaacattg
    21121 gtctcgaacc cctggagcca cggccaatcg gtgcaacagc gaaatgcctc ccgcgatgag
    21181 cagcaccaga aaaacgaccg tgatagggac ccatacgcgc ttgtggaccg atggcaatcg
    21241 gatgcgagag gtggcaatgg ccgcgcccaa gaaaataccg gcatcggcga ccgcgtgggc
    21301 aacttccggc tgaccggatt ctcgccgcgt tgcgacataa accgctgctc ccaccgctac
    21361 acaagccaac acaaaggccg tccacgctac cacgggccgc tggctgatgc tcctcacccc
    21421 cgcagccaat cgccatgcca ccaaggcgca aacggccacc acgccaccgc ccacgaccgc
    21481 aaacgtggtg gcgcccagac cgcgatgatg ggtgacggcg ctcaacaccg aaccaaccca
    21541 ctgnnnnnnn nnntgagcaa tgcgaccgac aggataatcc aaatggcgcc ggcaaaaagc
    21601 aaggtcgcac ggctggaggg atctggaaat gccttgtgta atccaccagt tgcggttctc
    21661 aggaaaaaaa tgatcacgac ggccagagcg gcccagaccc ccgccaacga aagccaggca
    21721 accccgaacc ccgcttgttg cgccacgcgc gatgacatgg caacggcagc gccactgccg
    21781 gtcaaaatcg atgccagcgc gatctctccg ctaatatgcg cgaagaggtc ccgcgtagga
    21841 aggggccaag tcgaatctgc cacgccccat tgcatagcaa agcaaagccc aaggaccaaa
    21901 tccccggcgc aaaaagcccc tcccaccccc tgcatcccgt tgtccgctca cgcaatcttt
    21961 cccgtgagtt atccccgtcg aacccatcga agcttcaccg tcgcgaccgt ggtccagtgc
    22021 caataggcaa gccaataggc atacgcaccc atcgaaagac gacaaagagg atgccctcga
    22081 tgattcaggc tttgcgcgtg acttttcgaa acgcctcgag acgaagggct tcttctttcg
    22141 ttttgggaat ccttccccgc caggcgggcg cgttgggcgg agcttgttgt ggacgccacg
    22201 atgcctgggc caaagccgat gccgatgctc gtgggccaag tttcctcact tcgtagtcga
    22261 cggcttccac cgcctgttcg acggtcgaga atcgttcgac caccttaccg tccatgctca
    22321 cttcccaacg cttgccgtga cccgcgcgag cgagcacgag cacttccgtg atttgtcccg
    22381 cgcgcttttc gaaatgcagc gccaaccctc gactgccaag agaaacccag ccgtgattgg
    22441 agatggctcg tacttcgccg gggagttgca agcctagcgg atcgaattgc ttcgctcgct
    22501 gctggatttc cctcagcgtg atctctccgg cctcccattc gaatcccgga taatcaaaaa
    22561 cgatgcgttc ataacgctgg ctcgctccta gcgcctcttc ccccaataga ttcaattcgt
    22621 agggcatgcc gaatagcgac ggcagcgtac acaaacgcag cttcgacaca tcgacgaaat
    22681 cgaggatcat acagtcgcgt ttgcctggaa atagccttgt gccacgaccc acacactggg
    22741 cgtacatccc ttccgagcgt gtgggacgag ccatcgccac acacgacacc ccagggtcgt
    22801 cgaaaccttc cgtgagcacc gcgacattgg tcaatacggt cgttttgcct tgtcgaaaac
    22861 tcgccagttc gctggctcga cgctctgatg gcatctgtcc gtggaccatc cccgatggaa
    22921 caccgagatg attgagtgct ttgcatagcg cgcgagcgtg agcgacggtg acgcaaaagg
    22981 cgatggtacg acgatctcgc gcgagttcct ggatcgaccg ggcgaccaga gcgttgcgct
    23041 cctcgacatt gatcacggcc tccaattcgt cttctcgaaa gtcctgaccg gaattggata
    23101 gttctcgaag atccgaggcc gtcgagattc gaaagccccg caatgggcaa agatagccgt
    23161 cgcgaatcat ctccggaagc gttcgcgtat agacgattcg ctcgaagatc tcgtggagcc
    23221 cctggccatc gccccgtgtg gtcgtggccg tgaaaccgag caaggtccct tgccaatccg
    23281 gttcgaatac accgagttgg gacagcacgc gacgattgtc ttgtgcggcg gcatgatggc
    23341 attcgtcata gatcacaagg ccaaaatgac gatcttgcat cacgcgcgaa agccgctgtt
    23401 catgaagcga gcgaatcgag caaacgagca cgtgagcatc ggccgaagcc cgatccattc
    23461 cgcgttcgat ggcaaccacc cgatggtcgc caagggcgtt ttgaatcttg tccctggctt
    23521 gcgtcagcaa ctcctcgcga tgagccagca ccagtaccgg acgtcgcgcc agacttgcaa
    23581 gatgtgaaaa tataacagtt tttcctgatc ccgtgggcag gcataccagc atgcgcttgc
    23641 atccctgtcg tcgcgcttca atcaccgcgg tcaccgtttc gcgctggtaa ggccgaagtg
    23701 tcatcgggaa ggcggaccgc gggaccatca cgacctcaga ctgtctcgaa gctgccgcac
    23761 tgtcatcagg aaatcagcaa gagccgttgc tttcggggat ggacgattcc catggggcag
    23821 gcttacgaag cgagcacaac gagatggcac cgagacggcc acg
//
LOCUS       DAOSOR010000016        23850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_2140_length_23850_cov_4.172515, whole genome shotgun sequence.
ACCESSION   DAOSOR010000016 DAOSOR010000000
VERSION     DAOSOR010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 23850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23850
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2140_length_23850_cov_4.172515"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..189)
                     /locus_tag="PK710_01625"
     CDS             complement(67..189)
                     /locus_tag="PK710_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08432.1"
                     /translation="MPSIVRAARPALEDPDCAAQPPSPTPEQAPPRNPNPSPPY"
     gene            complement(304..1200)
                     /locus_tag="PK710_01630"
     CDS             complement(304..1200)
                     /locus_tag="PK710_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08433.1"
                     /translation="MANKPVALVLHGLLSLSSCRLGEPLDGFLGGDDSSIDAAYASGG
                     SDSGSDGSDGGFGGSDGGFDGNVGAAAGSGGDDGGGTSGGGAAGSGGKETAGSGGLGG
                     LAGTGGGSNMVYGMIYQADFHTDFILDFNRLLDDQYVQDHENGVQQGPAFLGVYTTAY
                     APIPPAGQFTRAFATHALSEGLDVIQQTITDTQKYASPAVAVLFSSPVVTSGPIVLGP
                     TRQAPAQLFVMDVSDNFFVFCVAAIGLGLIEVTHAINTEAADGGELEFNGSNITLYHP
                     SNTPFGDIRAEIKKQFSVCSFQ"
     gene            complement(1552..2532)
                     /locus_tag="PK710_01635"
     CDS             complement(1552..2532)
                     /locus_tag="PK710_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT08434.1"
                     /translation="MSAAVIQVQHLSKTFRHGFFRKRVDALRGVSFEVGQGSIFGFLG
                     PNGAGKTTGIKILTGLITATDGQAWVLGKRVPAPEARRRLGFLPENPYVYPYLTPLEF
                     VTMCGRLSGMGAQALRKRTGQVLERVGIAYAADRPVQRLSKGMLQRTCLAAALVHEPD
                     LLILDEPMSGLDPVGRKEVRDIILEERAQGRTVFFSSHILSDVESMCDQVTILRKGQV
                     VVEGKIDALLHREGLFTDILLAGEPEALQQLHGRLEGKHGVLARPPEAVLRVEAKGDE
                     AVASVLSAALEGKVRVVEVAPRRETLEELFVKRAFSEESETTQAETAASS"
     gene            2827..5133
                     /locus_tag="PK710_01640"
     CDS             2827..5133
                     /locus_tag="PK710_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOT08435.1"
                     /translation="MKTATSWLPVLLAVGWSVQAQGQTPPTYYSPGSPSSEPAPAEPT
                     AAQPAAPAPQAQPAPATAPSAGASASGSWSFASGFDGDASGSGSAGAKAPMSDEQAYQ
                     WRLQSLKLHNNIYGSTGLLRISDAGSGAEGTFRIALLADWFTTSSFLCTEDRPCGKAT
                     EDDATHFGTSLSLSVTPLPYLEAFASFRSFANSNSEGRPELLQVLGDSIIGLKAFTPA
                     EPNKLFRFGGEAQLLLLNGTGGVGLDGKGTGLRLRALSTVDFAAMEDAIPMRIYGNLG
                     YRLDNSAQVVADTEEARGNMPITRIERFGLGINRTDFVEIGLGVEGTFEYIRPFLAYT
                     VDLPLNRQDYVCHKGKATTRAFHDACLGENQTFAYMPSRLTIGARGYPVLKGFAPMAA
                     LDIGVTGTSDFLEEVAPQAPWTLYFGLGYAFDTAEPEPIVKTQQVEKVVQLAPPPQFV
                     IRGKVFEKGTTTPVANAIITLPGSMAGGFATGSEGVFETRSVQPGAYTLSITAEGYHP
                     GQCTAQVETRQSAQDPFGVPPSPSADASATASSAPIITEITCELEALPRMGNIVGRVM
                     DAATSSGVANASVVVTDAQGKEHRVTTDGSGNFRLDGVVPGAVKVQTEAEKYLLSTES
                     ANVEPRKDAQVNINLNARPKQSNVVVTQTQIIIRKQINFETDSAVIKGDSTSLMQEIA
                     DVIARNPSIKLVEVQGHTDNTGTREHNQELSERRANAVKDWLVANGIEASRLEATGYG
                     QTRPIAPNVTAANRAKNRRVQFVIKERQ"
     gene            5146..5835
                     /locus_tag="PK710_01645"
     CDS             5146..5835
                     /locus_tag="PK710_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08436.1"
                     /translation="MHLRVIFLLFVSLLAASCEGKEPPGATMTPSTGLARASASAQEN
                     PIFPVTSSSTGALASSAGPPSSAVSSPAVSAASSAAVIDGKNETKPPHTSEFLESQGR
                     ALLDAIVFDDVSKANDFFFPREPFTPLKDVKEPDRYWKHLYRAYEQDIHKLHRRHRDW
                     SGVAFESIEPGTPPAWVPPGDEANKIGYHRSWGTKLRYRLEGKLYTVKIHTVISWQGR
                     WYITHLLPWKK"
     gene            5996..7456
                     /locus_tag="PK710_01650"
     CDS             5996..7456
                     /locus_tag="PK710_01650"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOT08437.1"
                     /translation="MNPVGLAVTLRVRMSAADANQSNGSVSPARILALFSDAATELMI
                     RLDGDEGSLRALQQVELLAPVYVGDYIEATGVLTKIGNTTRQLAFEARKVATYARGED
                     LALTAADALASPILVCRAIGTAVVPKAQQRRPRLVLAALGTRKDSSRLPAGSVLVTPP
                     PSIIVTPARQTPPEVMLAASILGGGVTKDHTPHIPATPEQAATEAKRCVDSGASIICL
                     GVDDTSADAGTVASRMTDYVAAIRAVTQAVIVVSAIRPGTMDASSFLALADCGADILT
                     ASLGSFNFGDATVQTSRTVVREVTTGLRERGIPVMAEAYELGHFEEAMLLGRDRVLPA
                     PLRLQFVFGVPGAIGAHETMLQFVSERIPKSALWFAAGVGRHQRRVTETAMRLGGHVR
                     LGLADNIYHRRGVLAETSALFIERSASFALNIGRQPVSPDRARVLLSLVPPPTEADTF
                     VAQDDSSTADAEPNVDAAENDQAPISSAEGNEADES"
     gene            7509..9914
                     /locus_tag="PK710_01655"
     CDS             7509..9914
                     /locus_tag="PK710_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08438.1"
                     /translation="MKLCWRIPWQARVASLVALAALGSRIRLPAPPDPTLRGVADVLG
                     ESVQGKVLAHEFLWESSRGAFSDAIFGRNVLFLAKTQPDGPRDLYRAAVRVTYEGRPI
                     GVSRVVNLTDTPFGDEQGLMAAGQYVAYATVAFGAVQQVTVLDLQGQEVQAESRLSRW
                     LALVSDWQSTGSWHGLGRTEVSANLQASSATMSFGRDVLNVSTDSIPNAFSVSLATGE
                     VVAKANESLPVSSMRLPQLAKSPILWAVDTVRGVTGPEFIAWLEDHVFDARDRVKRAG
                     HHAFGATEEGEEQAGPVAPPPPQPKKVEGNGQYAVAPWPPENLRSIWKTPEPDEGLWR
                     EPNAAFLQKNEVQGGEQPPAYFFQTYVRPDPKRPWSKVWLVAMDARQIQVGIEGGIED
                     PKPLTGARGEGRIPREPEILHRTVGAFNGGFKTTHGEYGMMVSRRVLLPAQPGAATVA
                     VTEDGRAAFGTWPLSTNIPEQFTSFRQNLDPLVDDGKLNPSNRKQWGWHLHTTGMLTH
                     RSGFCLTSAGQMVYAWGPEVTGETLGYAMIQAGCTYAIHLDMNPRHTAFAYIDARQRQ
                     QAKLLHPGMEVLPERFIVWSPKDFFYLTLRNFNLFAWEGVNMLPDKGRQPPPVWVPAV
                     FEGRSGKGSKTVQITAFRLNRLQARWVAEGEALETSEVEQALAAIVPSASSKGPGAML
                     ILSESGEISLVEEGKTMAGRAVRLPLLDEKEQGAEHVAMCITRQTLWVGRTMGTAAHL
                     AQTLRKSGCELVVSLGQGAAVHRTGTSEPTLDRYPTAALYLLGRSLSPGAFRWQPGQA
                     AAQ"
     gene            complement(10034..11119)
                     /locus_tag="PK710_01660"
     CDS             complement(10034..11119)
                     /locus_tag="PK710_01660"
                     /inference="COORDINATES: protein motif:HMM:NF017736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOT08439.1"
                     /translation="MNGKWIVRSAMALLLAVSSPAFAQQTHVVGQGHTLGKIAKRYNT
                     TVEALCDANQINRRTPLKVGQKLVIPDPPGTKKTSTPTTPPSASKAENTKPTPEKTEE
                     YTTHIVASGHTLGAISRRYRTTVEAIQQANNLQPRQKLRVGACLVVPLTRHALPRHRT
                     QKLPCQPEDSKDNSKESSSQTAPTPPAISPKPPSQSGVIHLVRGGSTFRGRVLDRKGH
                     PIPSAVEKIDSLLFDRRAGSTHSTDLGLIAKIAQVSDHFGGRRLIVVSGYRQESSNPY
                     TTRSNHALGRAIDFRIEGVSNEQLRDYCHTLTGVGVGYYPNSTFIHLDVRSITTHWTD
                     VSGPGEAPQYTTVTTPTPPRTKSRKRP"
     gene            11313..12398
                     /gene="pilM"
                     /locus_tag="PK710_01665"
     CDS             11313..12398
                     /gene="pilM"
                     /locus_tag="PK710_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOT08440.1"
                     /translation="MAEGKNLVGVDIGSSSIKVCELREGRKGHTLHKVAYAPLPQQTI
                     VDGHVMNTSIVIETLMRLFAEHKIKQRDIAVGVSGQSVIIRKITVPMMTAAELDEQIQ
                     WEAEQHIPFDIKDVQIDYQVLRRHPDRGLMDLLLVAAKRDEINDHAQFIREAKLRPVC
                     VDIDAFCVQNLFEVAVGIPPHQTIALLNMGASLTSLNIVSGGTSAFTRDITSGGNTIS
                     EEIQRETGMSFEQAEAYKCGDTSAGDGGAGAEQGGMVPQHVQQVIDSACDTIAAEIQR
                     SLDFFMATSGEAEINQIYVTGGTANLSSLRAAIQRRARTPVEFFNPVANLTIDHKHVR
                     QDIVQTRSAQLAVAIGLALRKDKEKRS"
     gene            12395..13030
                     /locus_tag="PK710_01670"
     CDS             12395..13030
                     /locus_tag="PK710_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOT08441.1"
                     /translation="MIRINLLPQKREARRDSGQGWLIATMLVVAAEVVVLGLFHQFKL
                     QELDKQKQINSELNNQIAQINTTVQNHAEVKKKLEVLRAREDAINKLQSARSGPTAVL
                     LELSRILTAGRGPTISAEELMQKKKDNPQDVHNASWDARRLWVLSFKENQRVVNLEGF
                     AHDGEDVSELARRLSLSKYFQDVVLLPASQQVDGESKLNVVRFQLQTKARY"
     gene            13034..13645
                     /gene="pilO"
                     /locus_tag="PK710_01675"
     CDS             13034..13645
                     /gene="pilO"
                     /locus_tag="PK710_01675"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOT08442.1"
                     /translation="MAAAKSSALSRIPTVAKFGIGLGFGVLTAIAYFIIFYSDVSSAI
                     RSAKSQEGTLRNQLADARKIEVAYQQDLGELNDRQQRQREFNKVLPESASYPAFLSSI
                     QGVANVSGVNLMAWAPMEESSQEFYAKVPMKLKLTGRFHQIAKFFYGVGQLDRIINIE
                     DIELLNPKVTDEDITIEVNCLATAFRSLPMRAPAAPEANPGGQ"
     gene            13647..14264
                     /locus_tag="PK710_01680"
     CDS             13647..14264
                     /locus_tag="PK710_01680"
                     /inference="COORDINATES: protein motif:HMM:NF016254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HOT08443.1"
                     /translation="MIARKPALLVALGLLLMAAAIGCDDDNKANLFSESEPAAPPPRR
                     GLPPAASASPDGGDDAESKLAFREEDFVETDLSRDPFRSFSKVFKQETASTARTQREV
                     LLERYAVDELRLVGIVTGGAEHRAMFVDPTGTGWIVHRGQFMGKAELVRGSGPGSASY
                     ELNWRVDRIRDGDVVLVREDPAHSDIPPATMVIPLRPEDSASASR"
     gene            14457..16754
                     /gene="pilQ"
                     /locus_tag="PK710_01685"
     CDS             14457..16754
                     /gene="pilQ"
                     /locus_tag="PK710_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HOT08444.1"
                     /translation="MRFSRGASWTVLRAWVIALAVSSTAFAKEPDNQVQQVTVQQDGP
                     KTIITVEASAPPLYHAQLVAGGKRLIIDISHADVVGPPPTITRPSGVVGGVLVQPFAS
                     SSGKTARMYIQLLAKATYQVRVRGNHLIVELQSSTESQAAQPADASARRTTASVTLQR
                     VQYQNDDRSDSVILHLSKQPAYSQSSPRTGVSRLLLRDTTVEQAAERILDASAFNGLV
                     RSISTYRSKQSRSDTVVEVERADNAVGIVHLNGNQLVWTFALPKDLPSSLTGVGADGG
                     AARKTRTVAREAALVDVPTVHSGDQLDPVEESEAEQAEAGAFLPQPTLGQAANTRAYR
                     GRRIDLDLKDADIHNVLRLLADVGRVNIITADNVSGSVTIRMRDVPWDRALNVVLQAK
                     SLGMTQEGNVIRVAPLAELEKEREMAIARRKQEYELTPIETRLIPVSYASAGEVQARA
                     KDLLSPRGSIAVDDRTNMLIARDVPGNLNQVEELIRSLDTQTPQVLVEARIVEATSRY
                     MRDIGVQWGGDATFSQATGNPTGLTFPSSVGIAGGASDGETPTAGLSPFNSRVQNPNF
                     AVNLPAAVGTGSGGAVGLTFGHINNTVNLALRLSAAETSGMVRIISSPRILTLDNHEA
                     SISQGTMIPFSQVSAMGVQTSFREAKLQLLVRPHVTADGSVSMRVKVNRDEPDFTQTS
                     ARGDPTILKREAETELLVMDGHTAVIGGIFTRNTGRNLDQVPFFGDIPLLGLLFQHRR
                     ASDSRGELVIFITPRIVNRAEALGR"
     gene            complement(16841..17326)
                     /locus_tag="PK710_01690"
     CDS             complement(16841..17326)
                     /locus_tag="PK710_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HOT08445.1"
                     /translation="MTEKSFEDGPIDDVQVRTLRPIEDERGWLCELYRRDEVDESVLP
                     AMSYASMTRPNVARGPHEHRDQTDWICFVGPSDFLLLLWDNRPWSATYRRRMRLVVGE
                     SNPAMVMIPPGVVHGYRNVGSRDGMVMNFPNRLYRGQGRTQAVDEIRHELDENTAYII
                     R"
     gene            complement(17326..18414)
                     /locus_tag="PK710_01695"
     CDS             complement(17326..18414)
                     /locus_tag="PK710_01695"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR01208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HOT08446.1"
                     /translation="MKGLILAGGVGTRLRPFTYSGAKQLVPVANRPVLYYVVDNLVDA
                     QITDLAVVISPETGREVKQSLGDGSAFGARLTYVLQDKPLGIAHAVKVARPFLQEDDF
                     VMFLGDNLIGTRIAPTVQRFARSPELSAFVLLKEVSDPSAFGVVKVDAQGNVMYLQEK
                     PKHPPSNLALVGVYLFRRVIHDAIDELSPSWRGELEITDAITRLMQRGGKVSVETLSS
                     WWLDTGKKDDLLLANDTVLDDWLKAGQEGQVDEDSKITGRVRVEKGAKVIRSTIRGPA
                     IIGRDAELIDARIGPFTSIGDRVKVHRSTVDHSVLMEECLVQDIPRLEDSLIGRRVIV
                     RPGASRHGALSLMVGDDCLVELSRERGG"
     gene            complement(18411..19469)
                     /gene="rfbB"
                     /locus_tag="PK710_01700"
     CDS             complement(18411..19469)
                     /gene="rfbB"
                     /locus_tag="PK710_01700"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOT08447.1"
                     /translation="MIRILVTGGAGFIGTNLVYRLLARPDVDLVVLDLLTYAGNLDNL
                     AALRHQPHFHFVHGDLADRVLLDELLREHRPDVIFNLASESHVDRSIDSPRPFVHTNL
                     VGTFELLDATRLYWSMLDREHRDRFRLLHVSTDEVFGSLGQEGAFSETTAYAPRSPYA
                     ATKAGADHLVRAFFHTYGMPTITTNCSNNFGPYQFPEKLVPLALLNALEGKTIPIYGD
                     GKHVRDWLHVHDHCDALLMVSERGKPGQTYNIGARNERSTLQMVETICALLDEWEPAV
                     RPYRDLIRFVMDRPGHDRRYAIDPSKMERELGWKPRISFEQGMRETVKWYLDNRVWCD
                     RITQRVYGRERLGLGASA"
     gene            complement(19574..20119)
                     /locus_tag="PK710_01705"
     CDS             complement(19574..20119)
                     /locus_tag="PK710_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOT08448.1"
                     /translation="MTTTEIKFGGLGGQGVILAGIITGKAAALFDNKEATLTQAFGPE
                     ARGSACSAQVVVSDTPILYPYVTRPRVLVSMSQDAYTKFSPQIAQGGMLLIEQDMVKP
                     DPIPNEAKVFGVPATRIAEELGRRMILNIVMVGFFTSITKLVSVDAMRKAVEDSVPRG
                     TTDLNLKAFDRGFQWGEKLIH"
     gene            complement(20116..21003)
                     /locus_tag="PK710_01710"
     CDS             complement(20116..21003)
                     /locus_tag="PK710_01710"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOT08449.1"
                     /translation="MTEVAINPVNPYLRMDRMPSIWCPGCGIGTSVNCFARALTQSKI
                     DLKKTAVVSGIGCTGRVSGYMSVDSFHTTHGRALAFATGLKLGNPELNVVVYSGEGDL
                     IAIGGNHFIHAARRNLDVLCVCVNNFTYGMTGGQVSPTSPATAKFTTAPLGQFEEPFN
                     LPYLADSCGAVYVARWTTFHVRELTKAMRDALKKRGFRFIEVISPCPTLYQRRNRLGD
                     GLDAMKYYQEKSVKKNGANTSELRIDFQSEITVGTFVDRERPTFADAMNARYKEVLGP
                     RYVPCPDNYVPPQAKGEGT"
     gene            complement(21019..22185)
                     /locus_tag="PK710_01715"
     CDS             complement(21019..22185)
                     /locus_tag="PK710_01715"
                     /inference="COORDINATES: protein motif:HMM:NF006412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOT08450.1"
                     /translation="MAADPRGVLTGQHYLDGNHAACEGALAAGCRFAAGYPITPSTEV
                     VERIAQRFPTMGGVFIQMEDEIAASITLQGGVWAGKKALTVTSGPGFSLMMEHVGLAA
                     ITECPCVFVNVQRGGPSTGLPTLPGQADMMQARWGSHGDYEIIALCPQSPQECFDFAI
                     KAFNLAETWRVPVMLMMDECVGHMTERVVIPNAEDIEVVERNFTKKKPGEYKPFDAGD
                     GWVPEMAKAGDGYNLFVTGLTHDERGYPVMNHHAQEKLVHRLVDKIRKNADKICEWEE
                     DGIEGAEVVVMSYGITSRVAQRGVEIAREQGIKVGTMRLIVAWPFPEKRVKQIAPTIK
                     SFVMPELNYGQMVFELERCVAGMTRTVLVPHGGGEVHDPQVIANAIIEAARGKN"
     gene            complement(22229..22486)
                     /locus_tag="PK710_01720"
     CDS             complement(22229..22486)
                     /locus_tag="PK710_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT08451.1"
                     /translation="MSDAGGAAKPKKVRGTVFVEVERCKGCGFCVEFCPPRALDLDTG
                     FNKKGYHPPVLTREEDCTGCDLCGLYCPDFAIFGVIIKKSA"
     gene            22901..23599
                     /locus_tag="PK710_01725"
     CDS             22901..23599
                     /locus_tag="PK710_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT08452.1"
                     /translation="MANVLIVEDEPAIAESLSFALRRDGFAVDIADCLAQVNGKIDLA
                     DLVLLDLMLPDGSGFDLIVQIRRRALRPAIIVLSSRDEEADRVAALESGADDYVTKPF
                     SPREVVARVRAVLRRAQPFEAPELHLSCSSIQVDVTTRRAVVQGTVLELTRVEFDLLA
                     CLIEEPGRVYTRSQLIDKVWGDGFAITDRTVDSHVKGLRKKMVEAGGDPNLVETVRGV
                     GYRIALVSSVQSEP"
     gene            23596..>23850
                     /locus_tag="PK710_01730"
     CDS             23596..>23850
                     /locus_tag="PK710_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HOT08453.1"
                     /translation="MIRFLFVFTGAIGLILLMAFVAAKLSRVRTEGISVRLQIFIALA
                     FIVGAFSFGLGLLVLDRIEARATRVAKEAASDEAAAIATFI"
BASE COUNT         5099 a         7163 c         6507 g         5081 t
ORIGIN      
        1 cgttcggccc cagaagtatg ctccgggcgt tgccccatcg tatggcatcg gtctttggcc
       61 gggttgtcag tagggggggc ttgggttggg gtttcggggg ggcgcctgtt ctggcgtggg
      121 gctgggcggt tgtgcggcgc aatccgggtc ttccaaggct ggacgagccg ctcggacgat
      181 ggacggcatc gctatccgta tccgtataca attcacgggc agggggtcca tgcatgccat
      241 gcatggtgat aaacgagcgg cggcagcacc tcgacgcggg tgatctgggg gtgttctggc
      301 cggtcattgg aaagaacaga ccgagaattg tttcttgatc tcggctcgga tgtcgccgaa
      361 gggagtgttg gacggatggt acaaagtgat gttggagccg ttgaattcga gttcgcctcc
      421 gtccgcggcc tctgtgttga tcgcatgggt cacctcgatc agcccgaggc cgatggcagc
      481 cacgcagaaa acgaaaaaat tatcagaaac atccatgacg aatagttgcg ctggggcctg
      541 tcgcgtgggg cccaggacga tggggccgct tgtcaccacg ggtgatgaaa acagcacggc
      601 aacagcggga gaggcatact tctgcgtgtc ggtgatggtt tgctgtatga catccaagcc
      661 ttcggagagt gcgtgggtag cgaacgcccg ggtgaactgt cccgcgggag gaatcggtgc
      721 gtaggcggtg gtatagacgc ccaagaaagc ggggccctgt tggacaccgt tttcatggtc
      781 ttgcacgtat tggtcgtcca gcagtctatt gaaatcgagg atgaaatccg tgtggaaatc
      841 ggcttgatag atcatgccat agaccatgtt ggaaccgccg ccggtgcctg ccaagcctcc
      901 aaggccgccg gagccggccg tttcttttcc gccggaaccg gctgctccgc cacccgacgt
      961 tcctccgcca tcgtcgccac ccgagcctgc ggcagccccc acgttaccgt cgaaaccacc
     1021 gtccgaaccc ccgaaaccac cgtccgaacc gtcggaacca ctgtccgagc ctccgctggc
     1081 ataggctgca tcgatggatg aatcgtcgcc ccccaaaaaa ccatccaatg gttctccaag
     1141 cctacaggat gataggctca gcagaccatg tagcaccagc gcaacaggct tattggccac
     1201 ggacgaaatg ttccttgcga gaacgctgct gtcaacaact gcctttccat gaccgtcctt
     1261 gcccttggaa cggggtgatg ttttcaggca aaaggtgaag aagccattgg cgagaggtgg
     1321 gcttgccagg aaggcatatt tcggggtgga tcgagtcgca ggggttggct gccgtgcatg
     1381 gagccaagct gctgtgaact cgatgtcctt cggtggtggt gggtgaagat gtcgaaatcg
     1441 cggcgcgccc gggcccctgc tggttggcgc gaacgctttt tgcctggtgg gcaaggtggg
     1501 aaacgaacct gaggagggtt gatttctgtc tatccattta tggataatcg atcacgatga
     1561 tgccgcggtc tccgcctggg tcgtctctga ttcctcggaa aacgctcgct tcacaaacag
     1621 ctcttccagg gtctctcttc gcggcgccac ctccaccacc cggaccttcc cttccaaagc
     1681 tgctgaaagc acggaggcaa ccgcctcgtc gcctttggct tccacgcgca gcaccgcttc
     1741 cggaggccgc gccaagactc catgtttccc ctcgagccgt ccatgaagtt gctgcaacgc
     1801 ttccggctcc cccgccaaca agatgtctgt gaacaggcct tctcgatgca gcagcgcatc
     1861 gatcttgcct tcgacgacga cctgaccttt tcgaaggatc gttacctgat cgcacatgga
     1921 ctcgacatcg ctgagaatat ggctcgagaa aaacaccgtt cgtccctggg cccgttcttc
     1981 gaggatgatg tcacgcacct ctttgcgccc caccggatcg agcccgctca tcggttcatc
     2041 gagaatgaga aggtccggtt catggaccag cgcggcggcc aggcacgttc gttgaagcat
     2101 gcctttggac agccgctgca cgggacgatc cgccgcgtag gcgattccta cccgttcgag
     2161 cacctgcccc gtgcgtttgc gaagggcctg ggctcccatt cccgaaagac gaccgcacat
     2221 cgtcacgaac tcgagtggtg taagatatgg ataaacataa ggattttctg gaaggaaccc
     2281 cagtcgacga cgtgcttcgg gcgctggcac gcgcttgccc agcacccacg cttgcccatc
     2341 ggtagccgtg atcaatccgg ttagaatctt gatgccggtc gttttgccgg cgccattggg
     2401 gccgagaaag ccgaatatgc ttccttgacc gacttcgaac gacacgcccc gaagagcgtc
     2461 gacccgtttt cgaaaaaaac catgccgaaa tgtcttggaa agatgttgaa cctgaatgac
     2521 cgcggcgctc acgaattagc ctctgcccca tcttactaca cggccccgaa agatgcgctc
     2581 cgacctgaac cgtcggctcg acaaccctca tgatccgcca caaaccatgc agattcggac
     2641 aagcccatcg aaaatggtcc gtaggacccc ttctgagccc agaaacgtca tcgagccctg
     2701 accggaagaa acgattcctt tgagcaactg tcccaaacag tggtacaacc gcgacccatc
     2761 gacccgtacc gtccctcttt gggggcgcaa accgaattgg gcatgtgagg agcgatgcat
     2821 acgaccatga aaactgccac ctcttggctt cccgtccttc tcgccgtggg ttggagcgtg
     2881 caagcgcaag ggcaaacccc gccaacctat tactcacccg gcagcccttc gtcggagcca
     2941 gcccccgcgg agcccactgc cgcgcaacct gctgctcccg caccccaagc acaacctgcg
     3001 cctgcaaccg ctccttccgc gggcgcatcg gcttccggaa gctggtcctt cgcttccggc
     3061 ttcgatggcg atgcttccgg ctccggctcc gctggcgcca aggctcccat gtccgacgaa
     3121 caggcttacc aatggcgtct tcagtctctc aaattgcata acaacattta tggttccacc
     3181 ggcttgttgc gcatttccga tgctggaagc ggtgcggaag gcaccttccg tatcgccctt
     3241 ctcgcggatt ggtttacgac atcttccttc ctttgcacgg aagataggcc ctgtggaaag
     3301 gccaccgaag acgacgccac gcacttcggt acgagtctat ccctatccgt tacaccactt
     3361 ccctatctcg aagcgtttgc atccttccgc tcgtttgcca attccaacag cgaggggaga
     3421 cccgaactgc ttcaggtgct cggagactcc atcatcggtc tgaaagcgtt cacacccgcc
     3481 gagcccaaca aactcttccg attcggtgga gaagcgcaat tgctccttct caacggaacc
     3541 ggtggtgtcg gactcgatgg gaaaggaact ggacttcgcc tccgcgccct atccactgtc
     3601 gacttcgccg cgatggaaga cgctatcccg atgcggatat acggcaacct cggctaccgc
     3661 ctcgacaata gcgcgcaagt cgtcgccgat accgaagagg cccgcggtaa catgcccatc
     3721 acacgcatcg agcgatttgg tttgggaatc aaccgcaccg acttcgtgga aatcggcctg
     3781 ggagtcgaag gtaccttcga gtatattcgt cccttcctcg cctataccgt ggatcttccc
     3841 ctcaaccgac aggactacgt ttgccacaag ggcaaggcca ccacacgagc gttccacgac
     3901 gcatgccttg gggaaaacca gaccttcgcc tacatgccat cgcggttgac catcggagca
     3961 cgggggtatc ccgtgctcaa aggttttgcg ccgatggctg ctctcgatat cggcgtgact
     4021 ggcacgagtg actttctcga agaagtcgcg ccgcaggcgc cgtggactct gtatttcggg
     4081 cttggatatg ctttcgatac ggccgagccc gaaccgatcg tcaaaaccca gcaggtcgaa
     4141 aaagtcgttc aactcgctcc acctccccaa ttcgtcatcc gtggcaaagt cttcgaaaaa
     4201 ggaaccacga cacccgtcgc caacgcgatc atcacattgc ctggaagcat ggccggcggt
     4261 tttgccaccg gttccgaagg cgtgttcgaa acgagaagtg tgcagcccgg tgcctatacc
     4321 ctgtccatca ccgcggaagg gtatcacccc ggacaatgca ccgcgcaggt ggaaactcgt
     4381 caatctgctc aagacccgtt cggcgttccc ccatcgccca gcgcggatgc ctcggcaacc
     4441 gcttcttccg cacccatcat caccgagatc acatgcgaac tcgaagccct gccgcgaatg
     4501 ggtaatatcg tggggcgagt catggacgcc gcaaccagct ccggtgtcgc caatgcctcc
     4561 gtcgtcgtga ccgatgcaca aggaaaagaa catcgtgtaa cgaccgatgg cagcggcaac
     4621 ttccgactcg acggggttgt ccccggtgcc gtcaaggtcc aaaccgaggc cgaaaagtac
     4681 ttgctgagca ccgagtccgc gaatgtcgag ccgagaaaag acgctcaggt gaacatcaat
     4741 ctcaacgctc ggcccaaaca atccaacgtc gtcgtcaccc aaacccaaat catcatccgc
     4801 aaacaaatca acttcgaaac cgattcggcc gtgatcaaag gcgactctac cagcctgatg
     4861 caagaaatcg ccgacgtcat cgctcgcaat ccgagtatca aactcgtcga agtccaaggg
     4921 catacggata acaccggcac gcgcgagcat aaccaggaat tgagcgagcg acgcgccaat
     4981 gcggtcaagg attggctcgt ggcaaacggc atcgaggcaa gtcgtctcga agcaaccggg
     5041 tatggtcaaa cacgacccat cgctcccaac gttaccgcag ccaaccgcgc caaaaatcga
     5101 cgcgtccaat tcgtcatcaa agaacgccaa tagccccatt ttatcgtgca tctccgcgtc
     5161 atcttccttc ttttcgtgag cctgctcgcg gcttcctgtg agggaaaaga accgcctggc
     5221 gccacgatga ccccttcgac cgggctcgcc agggcttctg ccagtgccca ggaaaaccca
     5281 atctttccgg ttacttcctc atccaccggc gcgcttgcct cctcggccgg gccaccgtcc
     5341 tccgcggttt cgagcccagc cgtttcggct gcctccagcg cggccgtcat cgatgggaaa
     5401 aacgaaacca agccccccca cacctcagaa tttctcgaat cacaaggtcg cgctttgctc
     5461 gatgccatcg ttttcgacga tgtttccaaa gcgaatgatt tcttttttcc ccgcgagccg
     5521 ttcaccccac tcaaagacgt caaagaaccc gaccgatatt ggaaacacct gtaccgcgcc
     5581 tacgaacagg atattcataa actccatcgg cggcaccgcg actggtccgg cgtcgccttc
     5641 gaatcgatcg agcctggaac cccacccgct tgggttccac cgggagacga agccaacaaa
     5701 atcggatacc atcgctcttg gggcacgaaa ctgcgctatc gtctggaggg caagctatat
     5761 acggtgaaaa tccataccgt catttcttgg cagggaagat ggtacatcac ccacttgctg
     5821 ccgtggaaaa agtaagctcg aagcgtttct gaatggcatt ttccccctga taacgttctt
     5881 cgcttgcgct gctgcttgac agttcccggc ccgcggcacc atttcgccat caccgatgca
     5941 gcgaatccca ccgggggctt ccaatcagcg cctagccctt gctatggtgg tcgccatgaa
     6001 ccccgtaggt cttgctgtta cccttcgcgt ccgcatgagt gcagcggacg ccaaccaatc
     6061 caatggctct gtcagccccg ccaggattct ggccctgttt tcggacgccg ccacggagct
     6121 gatgattcgt ctcgatggcg acgaaggctc gttgcgggct ttgcaacagg tggagctgct
     6181 cgcgccggtc tatgtgggcg actatatcga agccacggga gtgctgacga aaatcggcaa
     6241 taccacgcgt caactcgcct tcgaagcacg aaaagtggcc acgtatgctc gcggagagga
     6301 cctggctctt accgcggccg atgcgctggc ttccccgatt ctcgtttgcc gcgcgatagg
     6361 gaccgctgtc gttcccaaag cccagcagcg tcgaccgcgc ctcgttctcg ctgccctggg
     6421 aacccgaaaa gactcttccc gcctgcccgc ggggagcgtg cttgtcaccc cgccgcccag
     6481 tatcatcgtc actcccgctc gccaaacccc tccggaagtc atgttggctg cctccatctt
     6541 gggaggcggc gtgaccaaag accacacgcc gcatatcccc gccacgccgg agcaagcagc
     6601 tacggaggcc aagcgctgtg tcgatagcgg cgccagcatc atctgcttgg gtgtcgacga
     6661 taccagcgcc gatgcaggca ccgtcgcttc gcgtatgacc gattacgttg ccgcgattcg
     6721 agccgttacc caagccgtga tcgtcgtttc cgccattcgt ccgggcacca tggacgcctc
     6781 gtcttttctg gccttagcgg actgcggcgc ggatatcctc accgcttctt tgggatcctt
     6841 caacttcggg gatgctaccg tgcagacttc tcgaaccgtg gtgcgtgagg ttacgacggg
     6901 gctacgagag cgcggtatcc cggtcatggc agaagcttat gaactcggcc atttcgaaga
     6961 ggccatgctg ctcggacgcg acagagtgtt gcccgctcct ttgcggttgc aattcgtttt
     7021 tggtgttccg ggggccatcg gtgcccatga aaccatgctc caattcgttt cggagcggat
     7081 tcccaaaagc gctctgtggt tcgcggcggg cgttggtcgt catcaacgac gtgttaccga
     7141 gactgccatg cgtcttggag gccacgttcg cctggggttg gcagacaata tctatcatcg
     7201 ccgaggagtt ttggcagaaa catccgcttt gttcatcgag cgaagtgcct cctttgccct
     7261 gaacataggc cgacaacccg tcagtccgga ccgtgcgcga gtcttgcttt cgttggtgcc
     7321 gccccccacc gaggccgaca cgttcgtggc acaggacgat tcgtcgacgg ccgatgcgga
     7381 accgaacgtt gacgccgccg aaaacgacca agcccctatt tcctccgccg agggaaacga
     7441 agcggacgaa tcctaaatct gcaacgaatt tgacttaata gggtctcctt cgttagccaa
     7501 cggggtacgt gaagctctgt tggcgaattc catggcaagc aagggttgcc tccctcgtgg
     7561 cgctggctgc gctaggtagc cgtattcgtc tgccggcccc gcccgaccca actctccgcg
     7621 gcgttgccga cgtgctgggc gagtcggttc agggcaaggt cctggcccac gaattcttat
     7681 gggaatccag tcgtggtgcc ttctccgacg ctatcttcgg gcgaaacgtg ttgtttcttg
     7741 ccaaaactca gcccgatggc ccgcgggact tgtatcgtgc cgctgttcga gtcacctacg
     7801 aaggccgccc tatcggcgtg tctcgcgtcg tcaacttgac cgatacgcct ttcggtgacg
     7861 agcagggact catggccgcg gggcaatacg tcgcttatgc gaccgttgct ttcggtgctg
     7921 ttcagcaggt caccgtgctg gacttgcagg ggcaagaggt ccaggccgag agtcgattga
     7981 gccgttggct ggccttggtt tcggactggc aatcgacggg ttcttggcat gggcttgggc
     8041 gcacggaggt gtccgcgaac cttcaagcct cttctgccac catgagtttt ggacgggacg
     8101 ttctgaacgt ttcgacggat tcgattccga atgccttttc ggtttccctg gcgaccggcg
     8161 aagtggtggc caaagccaat gaatcactgc cggtatcgtc gatgcgcctg ccgcaactcg
     8221 cgaaatcgcc gattttgtgg gcggtggaca cggtccgggg ggttacgggg cctgaattca
     8281 tagcctggct cgaagatcat gtttttgatg cccgcgatcg ggtgaagcga gcggggcatc
     8341 atgcctttgg tgctacggaa gaaggggaag agcaggcggg tcctgtggcc ccaccgccgc
     8401 cccaacccaa aaaagtggag ggaaacggcc aatacgcggt ggctccttgg ccaccggaga
     8461 acttgcgttc gatttggaaa acgccggaac cagacgaagg cctgtggcgt gaaccaaacg
     8521 ctgctttctt acaaaaaaac gaagtccagg gaggcgaaca gcctcctgcc tattttttcc
     8581 aaacgtacgt tcgacccgac ccaaaacggc cttggtccaa ggtgtggttg gtcgcgatgg
     8641 atgctcgtca aattcaagtc ggtatcgaag gtggcatcga agaccccaaa ccccttacgg
     8701 gagcccgtgg agaaggacgc attccccgag aaccggaaat attgcatcgt accgtgggag
     8761 cgttcaacgg cgggttcaaa accacccatg gcgaatatgg gatgatggta tctcgacgcg
     8821 tattgttgcc ggcgcagcct ggggcggcca cggtggcggt gacggaagat ggccgggcgg
     8881 cgtttggaac ctggcccttg tccacgaata ttccggaaca atttacgtcg tttcggcaaa
     8941 atctcgatcc gttggtcgac gatgggaaac tcaatccttc caaccgaaag caatgggggt
     9001 ggcaccttca tacgacgggc atgttgaccc atcggtcggg tttttgcctc acatcggcag
     9061 ggcagatggt gtacgcgtgg gggccggagg tgacgggcga gacgttggga tatgcgatga
     9121 ttcaagcagg ttgtacctac gcgatccatc tggacatgaa tccgaggcat acggcgtttg
     9181 cgtatatcga cgctcggcag cgccagcagg cgaagttgtt gcaccccggc atggaagtac
     9241 ttccggagag atttatcgtc tggtccccca aggacttctt ttatctaacc ctacggaatt
     9301 tcaacctttt cgcgtgggaa ggggtgaata tgcttccgga caagggccgt cagccgccgc
     9361 cggtttgggt gccagccgta ttcgagggac gaagcggcaa ggggagcaag acggtccaaa
     9421 tcaccgcatt ccgtctcaat cggttgcaag cccgctgggt agcggagggc gaggcgcttg
     9481 aaacctccga agtcgaacaa gctcttgcag cgattgtccc ttcggcttcc tccaaggggc
     9541 ccggagccat gcttatcctt agtgaatccg gggagatttc gctggtggaa gaaggaaaaa
     9601 ccatggcagg acgcgccgtg cgattgccct tgttggatga aaaagagcaa ggggcggaac
     9661 atgtggccat gtgtattaca cggcagacgt tatgggtggg gcgtaccatg ggtacggcgg
     9721 cgcatttggc gcaaaccttg agaaagtcgg gatgtgaact cgtggtttcc ctagggcaag
     9781 gggctgccgt acatcgcacg ggaacatcgg agcccactct ggaccgttac cctacggctg
     9841 cgctctatct gctcggacgt tccctatcgc cgggcgcttt tcgatggcag ccaggtcaag
     9901 ctgccgccca atagatgggt caattttgac ctggacaatg gggatacacc gaactcaatc
     9961 cccgaatcaa cgaattacca caataatttc aatagattgg gcgctggaaa atgcggtcga
    10021 aaatgacccg ggatcaaggt cgttttcgtg atttggttcg cgggggagtg ggggttgtca
    10081 ccgtggtata ttgaggagct tcacctggtc cggatacatc tgtccagtgg gtggtgatcg
    10141 aacgaacatc caaatgaatg aatgtgctgt tgggatagta cccaacccca actccggtga
    10201 gcgtatgaca atagtctcga agctgctcgt tgctcacccc ctcgattcga aaatcgatcg
    10261 cccgtcccag cgcatggttg gagcgagtcg tataaggatt cgaagattct tgacgatatc
    10321 cgctcaccac gatgagcctt cgtcctccga aatggtcgct cacttgcgct atcttggcaa
    10381 tcaatccaag gtcggtggaa tgggtgctgc ctgcccggcg atcgaataat agactgtcga
    10441 ttttttctac tgccgacgga atcgggtgcc cttttctgtc aagaacccgg ccgcgaaatg
    10501 tacttccccc gcgaaccaag tgaatcacgc ccgattgcga tgggggctta ggagagatgg
    10561 ccgggggcgt tggtgccgtt tggctgcttg attctttcga attgtctttg gaatcttcgg
    10621 gctggcaggg cagcttttgt gtgcgatgcc tgggcagtgc gtgacgggtc agcggaacca
    10681 ccaaacaggc tccaacacgc aatttctggc gtggctgtaa gttgttcgct tgctgaatcg
    10741 cctcgacagt ggttcggtaa cgacgggaaa tagctccgag ggtatgaccg gaagctacga
    10801 tgtgggtggt gtattcttcc gttttttctg gtgttggctt ggtgttttcc gccttggagg
    10861 cgctgggggg agttgtcgga gtcgaggttt ttttcgtgcc gggcggatcc ggtatgacga
    10921 gtttttgtcc gactttcagg ggggtgcgac gattgatttg gttcgcgtcg catagggcct
    10981 cgaccgtggt gttgtagcgt ttcgcgattt ttccgagggt atggccctgc ccaaccacgt
    11041 gggtttgttg tgcgaaggct ggagacgaca cggccagtag caaagccatg gccgagcgga
    11101 cgatccactt cccgttcatg cgccgatgaa gaaacaacat gggaaagagc tgcacttgcg
    11161 tgctgccaag taccagactt agcgcccctg gccacctgat gcaaagcgct tttttgacca
    11221 actcacccat tgaatgccac acttgctttt gaacgattct gtttgggcat ggcaatcgcc
    11281 caaaacaaaa cccggcagca ggatggtgac cgatggcgga aggtaagaac ttggtcggcg
    11341 tagacattgg atcaagctcg atcaaggtct gcgaactcag ggaaggccgc aaaggacaca
    11401 ccctgcataa ggtggcctac gccccgctcc cccagcagac gatcgtcgat ggccacgtca
    11461 tgaatacgtc gatcgtcatc gaaacgctga tgcgattatt tgcggaacac aaaatcaaac
    11521 agcgagatat cgccgtgggg gtgtccgggc aatcggtgat catccgaaaa attacggtcc
    11581 ccatgatgac cgccgccgag ttggacgagc agatccaatg ggaagcggaa cagcacattc
    11641 ctttcgatat caaggatgta caaatcgatt atcaggtgct tcgacggcat ccggaccggg
    11701 ggctgatgga cttgcttttg gtggccgcaa agcgtgacga aatcaatgac cacgcccaat
    11761 tcatccgaga agcaaaactt cgacccgtat gtgtcgatat cgatgctttc tgtgtccaaa
    11821 acctattcga agtcgccgtg ggtatccctc cccatcagac cattgccctg ctcaacatgg
    11881 gagccagtct gacctccttg aatatcgtat ccggtggaac cagtgcgttc acacgcgaca
    11941 tcaccagcgg gggcaatacg atcagcgagg agattcaaag agaaacaggc atgagcttcg
    12001 aacaagcgga agcctataaa tgcggagaca ccagcgcggg cgatggggga gccggcgccg
    12061 aacagggtgg catggtgcca caacacgtcc agcaagtgat cgattccgct tgcgacacca
    12121 tcgccgctga aatccaacgc tccctcgact ttttcatggc caccagcgga gaagccgaaa
    12181 tcaaccaaat ctacgttacg ggaggcaccg cgaacctgtc gagcctgcgt gccgccatcc
    12241 aacgtcgcgc tcgtaccccc gtcgaattct tcaatccggt ggctaacctt accatcgacc
    12301 ataaacatgt ccgacaggac atcgtgcaaa cacgaagcgc gcagctagcc gtcgccatcg
    12361 ggctagccct gcgtaaagac aaggagaaac gctcgtgatc cggatcaacc tgctaccgca
    12421 aaaacgcgag gcgaggcgcg actccggaca ggggtggctc atcgccacca tgctggtggt
    12481 cgccgccgaa gtcgtcgttc tcggactgtt ccaccaattc aaattgcaag aactggataa
    12541 acaaaaacaa atcaactccg aactcaataa ccaaatcgca caaatcaata ccaccgtgca
    12601 gaaccatgcc gaggtcaaga aaaaactcga ggtgctgcgc gcgcgagaag atgccatcaa
    12661 caaactgcaa tccgctcgct cagggcctac cgccgtcttg ttggagctat cccgtatcct
    12721 taccgctgga cgtggcccca ccatctccgc ggaagaactc atgcaaaaaa agaaagacaa
    12781 cccgcaagac gttcataacg caagctggga tgctcggcgc ctttgggtcc tatccttcaa
    12841 agaaaaccaa cgcgtcgtca atctcgaagg cttcgcccac gatggggaag acgtctccga
    12901 attggctcga agactgtcct tgtccaaata cttccaagac gtcgtgttgt tgccggcttc
    12961 gcaacaagtc gacggcgaat caaaactcaa cgtggttcgg tttcagctcc agaccaaggc
    13021 aaggtattga gccatggctg ccgcaaagtc ctccgctttg tctcgcatcc ccaccgtcgc
    13081 caagttcggt atcggccttg gtttcggggt gctgactgcc atcgcctatt tcatcatttt
    13141 ctacagcgac gtttcgagtg ccattcgcag cgccaaatcc caagaaggaa cgttgcgaaa
    13201 tcaactcgcg gacgctcgaa aaatcgaggt cgcttatcag caagaccttg gggaactcaa
    13261 tgaccgtcag caaagacagc gggagttcaa caaagtcctg cccgaatccg cttcctatcc
    13321 cgccttcctg tcgtccatcc aaggtgtggc gaacgtgtcg ggcgtcaatc tgatggcctg
    13381 ggcccccatg gaagaatcct cccaagagtt ttatgcaaaa gtgccgatga agctgaaact
    13441 aaccgggcgc tttcatcaaa ttgcgaagtt cttttatggt gtcgggcaac ttgatcgcat
    13501 catcaacatc gaggatatcg agttattgaa ccccaaagtg accgacgagg acatcaccat
    13561 cgaggtcaat tgccttgcta ccgcattccg cagccttcct atgcgggctc cggctgcgcc
    13621 cgaggcgaat cccggagggc agtgatatga tcgcacgcaa gcccgccttg ctggtcgcac
    13681 taggtttgct gctgatggcc gcagccattg gttgcgacga cgacaataag gcgaacctat
    13741 tttccgaaag tgaaccagcc gcgccaccgc cacggcgagg cctgcctccc gccgcttccg
    13801 cgagccctga tggcggtgac gatgccgaaa gcaaactcgc gtttcgtgaa gaagattttg
    13861 tcgaaacgga tctgtctcgg gacccatttc gttctttctc caaggtattc aaacaagaaa
    13921 cagcttcgac agcacggacc caacgcgagg ttctgctcga acgatacgcc gtcgatgaac
    13981 tccgactggt cgggatcgtg accggagggg cagaacatcg cgccatgttc gtcgacccta
    14041 ccgggacagg ctggatcgtg catcgcggac agttcatggg caaagccgaa cttgtccgag
    14101 gctccggccc cggcagcgcc agctacgaac tcaactggcg cgtggaccga atccgcgacg
    14161 gtgatgtcgt gctcgtccgt gaagaccccg ctcatagcga tatcccaccg gcaacgatgg
    14221 tcatccccct tcgacccgaa gactccgcct ccgcgagccg ctgaatccgt tgccctatcc
    14281 cttgccacga aagccggtca tttcctctcg aatcacgtca tttcccgcaa tcgtcacgcc
    14341 cgggccaatt cattgtggcc acgaatttgg tccgttggtt cgagggagga tacgattgca
    14401 aagtagctgt ggcttggatt ccgcaatccg cggattccgt cgcacgggag ccaaagatga
    14461 ggttttctcg aggagcgtcc tggactgttt tgcgggcatg ggtcatcgcg ttggcggttt
    14521 catccacagc cttcgccaaa gaacccgaca accaggtcca acaggtcact gtccaacaag
    14581 atggtcccaa aaccatcatt accgtcgagg ctagcgcacc gcctctgtac cacgcacaac
    14641 tcgtcgcagg aggcaaacga ctcatcatcg acatctccca tgccgatgtg gtcggtcccc
    14701 cgcccacgat cactcgtcca agtggcgtgg taggcggcgt gctcgttcag ccttttgcct
    14761 cctcctcagg aaaaaccgcg cggatgtaca ttcaactcct cgccaaagcc acctaccaag
    14821 tgcgtgtccg aggcaatcac ctgatcgtcg aactccagtc ttcgactgaa tcgcaggcgg
    14881 cccagcctgc cgatgcttcc gcgcgtcgaa cgacggccag tgtcacgctc caacgcgttc
    14941 aataccaaaa cgacgatcgc tccgattccg tgatcctgca tctttccaaa caaccagcct
    15001 attcgcaaag ctcacctcgg accggcgtgt cccgcctttt gttgcgcgat acaaccgtcg
    15061 aacaagccgc ggaacgcatc ctcgacgctt ctgctttcaa tggactcgtt cgatccatat
    15121 ccacctatcg ctccaaacaa tctcgctccg atacggtcgt cgaagtggaa cgtgccgata
    15181 acgccgtggg tatcgtccac ctcaatggta atcaactcgt ttggaccttc gctctaccca
    15241 aagacctgcc gtcctccttg accggtgtgg gcgccgatgg gggagctgcc cgcaaaaccc
    15301 gcaccgtcgc ccgagaagcg gctctcgtag acgttcccac cgtgcactcc ggtgaccaac
    15361 tcgatcccgt cgaggaatcc gaggcagaac aagccgaagc gggcgcgttt cttccccagc
    15421 ccaccctcgg ccaagctgcc aatacccgcg cttaccgcgg ccgacgcatc gacctggacc
    15481 tcaaagatgc cgatatccat aacgttttgc gtctgctggc cgatgtcgga agggtcaata
    15541 tcatcaccgc cgataacgtc agcggtagtg tcaccatccg gatgcgagac gtaccgtggg
    15601 atcgagcctt gaacgtggtt ttgcaagcca aatccctggg catgacccag gaaggcaatg
    15661 tcattcgtgt cgcacccctc gcagaactcg aaaaagaacg agagatggcg attgcgagac
    15721 gtaaacaaga atacgaactc acccccatcg aaacacggct catccccgta tcctacgctt
    15781 ccgcgggaga agtccaggct cgggccaaag acctgctttc cccacgcggc tccatcgccg
    15841 tcgatgaccg aacgaatatg ctcatcgctc gcgacgtgcc gggaaatctg aaccaagtcg
    15901 aggagttgat ccgatcgttg gatacccaga ctccgcaagt gctcgtggaa gcgcggattg
    15961 tggaggccac gagccgctac atgcgtgaca ttggcgtaca atggggcgga gacgccacct
    16021 tttctcaagc gacgggcaat cccaccggac ttacctttcc ttccagcgtg ggcatcgccg
    16081 gtggtgcctc cgacggtgaa acccccactg cggggctatc acccttcaat agccgcgtgc
    16141 aaaaccccaa ttttgccgtc aacttgcccg ctgccgtggg caccggctcc ggtggcgccg
    16201 tcggtttgac cttcggtcat atcaacaaca ccgtcaatct cgcccttcgt ctctcggccg
    16261 cggaaacgag cggtatggtc cgcatcatct ccagcccacg catcctgact ttggataacc
    16321 atgaagcctc catcagccaa ggcaccatga tccccttctc ccaggtcagc gccatgggtg
    16381 tccaaaccag cttccgagaa gccaaactcc aactgctcgt tcgcccccac gttaccgccg
    16441 atggaagcgt gtccatgcgc gtcaaagtca accgagatga gcccgacttc acccaaacgt
    16501 ccgcacgagg cgaccccacc atcctcaaac gagaagcgga aaccgaactg ctcgtcatgg
    16561 acggacatac cgctgtcatt ggtggtatct tcacgcgaaa taccggacga aatctcgatc
    16621 aggtgccgtt tttcggagat atcccactgc tcggactgct ctttcaacac cgccgtgcaa
    16681 gcgattcccg cggagaactc gtcattttca tcaccccccg catcgtgaac cgggccgaag
    16741 ccctcggtcg ctagtcgctc gcctcacaat ccgtacccat ccaatacgtc tgcgccaatc
    16801 ccttaggatc cctatctatc cgtattcgga tagtttggat ctatcgaatg atatatgccg
    16861 tgttttcgtc caattcgtga cggatctcat ccaccgcttg cgtcctaccc tgcccgcgat
    16921 agagccgatt cggaaaattc atgaccatcc catcgcgcga tcccacgttg cgataaccat
    16981 gcactacccc cggcgggatc atcaccatcg ccgggttgct ttctcccacc accagccgca
    17041 tccgcctccg atacgtcgcc gaccatggtc gattgtccca aagcaaaagc aaaaaatccg
    17101 atggccccac aaaacatatc caatccgtct gatcccgatg ctcatggggc ccccttgcca
    17161 cattcggtcg cgtcatcgaa gcatacgaca tcgccggcaa cacgctttca tcgacttcat
    17221 ctcgtcgata cagttcgcat agccatcccc tttcgtcttc tatcggacgc aacgttcgaa
    17281 cctgcacgtc atcaatcggt ccatcctcga acgatttttc ggtcatcatc cgcctctttc
    17341 ccgcgacaac tcaacgagac aatcgtcgcc caccatcaaa gacaaagccc cgtgacgaga
    17401 tgcgccggga cgcacgatca cgcgacgtcc tatcaaagaa tcctccaaac gggggatgtc
    17461 ctgcacaagg cattcttcca tcaataccga atgatccacc gtcgaacgat gtactttgac
    17521 gcgatcccca atcgaggtaa acgggccaat ccgtgcatcg atcagctccg catctcgtcc
    17581 gatgatcgct ggacctcgga tcgtcgaacg aatgaccttc gccccttttt ccacacgaac
    17641 ccttcccgtt atcttgctgt cctcatcgac ttgtccttct tgacccgcct tcaaccaatc
    17701 gtcgagaacc gtatcgttgg ccagcagtag atcatctttt ttacctgtat cgagccacca
    17761 agacgaaagg gtttctacac tcactttccc tccccgttgc atgagccggg tgatcgcatc
    17821 ggtgatttcg agttctcctc gccacgaagg cgaaagctcg tcgatggcgt cgtgaatcac
    17881 acgacgaaac aaatacaccc ccacgagcgc caggttggag ggagggtgtt tcggcttctc
    17941 ttgcaaatac atcacattgc cttgggcgtc gaccttcacc accccgaaag cagagggatc
    18001 ggatacctct ttgagcaata cgaaagcgga tagttcggga gatcgagcaa accgctgaac
    18061 ggtcggcgcg atacgagtgc caatcaggtt atcccccagg aacatgacga aatcatcctc
    18121 ttggaggaaa gggcgcgcca ccttgaccgc atgggcaatc cccaacggtt tgtcttgcag
    18181 cacgtacgtc agtctggcgc caaatgccga tccgtctccg agcgattgct tcacctctcg
    18241 acccgtctcc ggcgagatga caaccgcaag gtcggtgatt tgcgcatcga caaggttatc
    18301 caccacgtaa tagagcaccg gacgattcgc taccggcacg agttgcttgg cacccgaata
    18361 ggtaaatggt cgaaggcgtg ttcccacgcc acccgcaagg atcaaccctt tcacgcggaa
    18421 gctcccaaac cgagtcgttc tcggccataa acgcgttgtg tgatgcgatc gcaccaaact
    18481 cgattatcca agtaccattt caccgtttca cgcatgcctt gctcaaagga aattcgcggt
    18541 ttccatccaa gttcccgctc cattttcgac ggatcgatcg catatcgacg atcatgcccg
    18601 ggtcgatcca tcacgaaacg aatgaggtcc cggtacgggc gaaccgccgg ttcccattcg
    18661 tcaagcaaag cacaaatcgt ctcgaccatc tgcaacgtcg aacgctcgtt tcgcgcacca
    18721 atgttatacg tttgccctgg cttgcctcgc tccgaaacca tgagcaacgc atcgcaatgg
    18781 tcatgcacat gaagccaatc gcgcacgtgc ttgccatctc cataaatcgg tatcgttttt
    18841 ccttcgagcg cgttgagcaa ggcaagcggc actagctttt ccggaaactg gtaaggcccg
    18901 aaattatttg agcaattcgt cgtgatcgta ggcatcccat aggtgtggaa aaacgcgcga
    18961 acgagatgat ccgctcccgc tttcgtcgcc gcatacggtg aacgtggcgc atacgccgtc
    19021 gtttcagaaa atgctccctc ttgtccaaga gagccgaata cttcatccgt ggaaacgtgt
    19081 aataaccgaa agcgatcacg atgctcccgg tcgagcatcg accaatacaa gcgggtcgcg
    19141 tcgagtaatt cgaatgttcc taccagattg gtgtgaacga acggacgcgg gctgtcgatg
    19201 gagcgatcga cgtgagattc cgacgccaga ttgaaaatca cgtcgggtcg gtgctctcgc
    19261 aacaactcgt cgagcaagac gcgatccgcc aagtctccat gcacgaagtg gaagtggggc
    19321 tgatggcgca aggcggcaag gttatcgaga ttgccggcgt aggtgagcag gtcgagcacg
    19381 accagatcga catccgggcg agccaacaga cgatagacga gattggttcc gataaatcct
    19441 gctccgccgg taaccaagat tcgaatcatg gttgtgctta gcctgaagga ccaaggggat
    19501 cgatgcaaac cggaaccata aacaatgtcc ctggttcaga tgagaatcat ctacggaaag
    19561 aaatgagaaa gattcagtgg atgagctttt cgccccattg gaaaccgcgg tcgaaggctt
    19621 tgagattgag gtccgtcgtt ccgcgcggaa ccgagtcttc caccgccttt cgcatggcat
    19681 ccacactgac aagcttggtg atcgatgtga aaaaaccgac catgacgatg ttgaggatca
    19741 tgcgtcgccc aagttcttcc gcaattcgcg tggcgggcac accaaagact ttggcttcat
    19801 tgggaatggg gtcaggcttg accatgtcct gctcgatgag cagcattccc ccctgggcga
    19861 tttggggtga aaacttggtg taggcatctt gggacatact caccaacacg cgcggacgcg
    19921 tcacataagg gtacaggatg ggggtatcgg agacgaccac ctgggccgaa cacgcgctac
    19981 cacgagcctc cggaccaaac gcttgggtga gcgtggcttc cttgttgtcg aaaagagcgg
    20041 ctgcctttcc ggtgatgatg ccagccagga tgacaccttg gccgcccaga ccgccgaact
    20101 tgatctccgt ggtcgtcatg ttccttctcc ctttgcctgc ggcggcacgt agttgtccgg
    20161 gcacggcacg tatcgcggcc ccaacacttc cttgtagcga gcgttcatgg catcggcaaa
    20221 ggtggggcgt tcgcgatcga cgaacgtgcc gaccgtgatt tcggattgga agtcgatccg
    20281 caactcactg gtattggcac cgttcttttt gacggatttt tcttgataat atttcatcgc
    20341 gtcgaggcca tcgccgagtc gattgcgacg ctggtagagc gtggggcagg gggaaatgac
    20401 ctcgatgaag cggaacccac gttttttgag ggcatcacgc atggccttgg tgagttctcg
    20461 cacgtggaag gtggtccatc gagcgacata gaccgcaccg caggagtcgg cgaggtaggg
    20521 cagattgaaa ggctcctcga attgtccgag gggcgcggtg gtaaattttg cagtggctgg
    20581 cgaggtgggg ctcacctgac ccccggtcat gccgtaggtg aagttgttga cacaaacaca
    20641 cagcacgtcg aggtttcgcc ttgcggcatg gatgaagtgg tttccaccaa tggcgatgag
    20701 gtctccctcg ccggaatata cgacgacatt gagttcggga ttgccgagtt tcaagccggt
    20761 ggcaaaggcc aacgcgcgtc cgtgggtggt gtgaaacgaa tccacggaca tatagccgga
    20821 tacccggccc gtgcagccga tgccggagac gaccgcggtt tttttcaggt cgattttgga
    20881 ttgggtcaac gctcgcgcaa agcagttgac gctcgttccg atgccgcatc ccggacacca
    20941 aatgctcggc atgcgatcca tgcgaaggta cgggttgact ggattgattg caacttctgt
    21001 catggccgat cctttgcgtc agttcttgcc gcgagcggct tcgatgatgg cattcgcaat
    21061 gacttgcgga tcatgcacct cgccgcctcc atgaggcacc aacaccgtac gtgtcatgcc
    21121 cgccacacaa cgttcgagtt cgaacaccat ttggccgtag ttgagttcgg gcatcacgaa
    21181 tgacttgatc gtgggcgcga tctgtttgac ccgcttttca gggaagggcc acgcgacgat
    21241 caggcgcatc gttcccactt tgattccttg ttctcgggcg atttctacac cccgctgggc
    21301 cacgcgcgac gtgattccat acgacatcac gacgacttcc gcgccctcga tgccgtcttc
    21361 ttcccactca cagatcttgt ctgcgttttt gcgaatcttg tcgacaaggc gatgaacgag
    21421 tttttcctgg gcgtgatgat tcatgaccgg ataaccgcgc tcgtcgtgcg tcagtcctgt
    21481 cacgaatagg ttgtagccat cgcccgcctt ggccatctca ggcacccaac cgtcgcccgc
    21541 atcgaaaggc ttgtattcgc caggcttctt tttggtgaag ttgcgctcca ccacctcgat
    21601 gtcctctgcg ttgggaatca ccacacgctc ggtcatgtgc cccacgcatt catccatcat
    21661 caacatgacc ggtacacgcc aggtttccgc aagattgaac gctttgatgg cgaagtcgaa
    21721 acactcttga ggcgattgcg gacacaaggc gatgatctcg taatcgccgt gggaccccca
    21781 tcgggcctgc atcatatccg cttgccccgg taaggtggga agcccggtcg acgggccgcc
    21841 gcgctgcacg ttgacgaata cgcaaggaca ttcggtgatg gccgcaagtc ctacgtgctc
    21901 catcatcaac gaaaagccag ggccggatgt gacggtgaga gcttttttgc ctgcccatac
    21961 gccgccttgc agcgtaatgg atgccgcgat ctcatcttcc atttgaatga atacgccccc
    22021 catggtgggg aaacgctgcg cgatacgctc cacgacttcc gtcgagggcg tgatgggata
    22081 ccccgcggcg aagcgacagc ctgctgctaa tgctccttcg catgccgcat ggtttccgtc
    22141 caagtaatgc tgacccgtca atactccacg tgggtctgct gccatggcga ccacctccgt
    22201 atgggaacga accccgacaa cgactgtctc acgccgactt cttgatgatc accccaaaaa
    22261 tggcgaagtc cggacagtaa aggccacata gatcacaacc ggtacaatcc tcctcgcgag
    22321 tcaataccgg cgggtgatac ccctttttgt tgaatcccgt atccagatcc aaggcgcggg
    22381 gaggacagaa ttcgacacaa aatccgcagc ctttgcatcg ctccacctcg acgaataccg
    22441 tccctcgcac cttcttgggc ttggctgccc ctccggcgtc agacatcttt catctctccc
    22501 tgtctcccgc accacgcggg ccgatcacgc tcagccgtcg aatccgcgga ctcgatccga
    22561 cgttctcctc cacggacgct gcttgttttt ttcgaacggg tccgtccaca ggcaggcctt
    22621 ctacctcgcg tcagttctat tcgcgaaata gaacgtgacc acgattccgt caacacaccg
    22681 tgacccgtca cagattgtct aatcgtgaat tgattatgga tgcttcctcg tagcaagttc
    22741 gactagatat ttaatattat aaattcatct tgttactgat ttgtggcgca ttgaaatcgt
    22801 gacgtgtgcg agcggttcgg acgctctccc ttgctttgaa cctccggtgc ttcgctcagg
    22861 ctccgcaccc ccccacaatt gtgctttatt gcctttcacc atggccaacg ttctcattgt
    22921 cgaagatgag cctgccatcg ccgaatctct ttctttcgcg ttgcgtcgcg acggttttgc
    22981 cgttgacatc gccgactgcc ttgcccaggt caatggcaaa atcgaccttg ccgacctcgt
    23041 tttgctcgac ttgatgctgc ccgatggcag tggtttcgat ctcatcgtgc agattcgtcg
    23101 acgtgcgtta cgtcctgcca tcatcgtgtt gtcaagccgc gatgaggagg ccgatcgggt
    23161 ggcagctctc gagtcgggag cggatgatta cgtgaccaaa cctttttctc cgcgggaggt
    23221 ggtggcgcgc gtgcgggcgg tgcttcgacg cgcccagcct ttcgaagctc ctgagctaca
    23281 cctgtcttgt tcgagcattc aagtcgatgt gacgacacgc cgcgctgtgg tgcaagggac
    23341 ggtgctcgag ttgactcggg tggagttcga cttgctcgcg tgtttgatcg aagagccggg
    23401 aagggtgtac acgcgctcgc aactcatcga caaggtgtgg ggcgacggct ttgccattac
    23461 tgatcgaact gtggactcgc atgtcaaagg attgcgcaag aagatggtgg aggcaggagg
    23521 agatccgaat ctagtcgaga ccgttcgtgg tgtggggtat cgcatcgccc tcgtttcttc
    23581 cgtgcagagc gaaccgtgat tcgattcctt ttcgttttca ctggcgccat cggtttgatt
    23641 cttctgatgg ccttcgtagc cgcgaaactg tcccgggttc gcaccgaagg catctccgtt
    23701 cgtttgcaaa tcttcattgc gctggcgttc attgtcggag ccttttcttt tggactgggt
    23761 ttgctcgttc tcgaccgtat cgaagcgcgc gccactcgcg ttgccaaaga agccgctagc
    23821 gacgaggcgg ctgccattgc cacgttcatt
//
LOCUS       DAOSOR010000017        23315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_2212_length_23315_cov_4.980138, whole genome shotgun sequence.
ACCESSION   DAOSOR010000017 DAOSOR010000000
VERSION     DAOSOR010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 23315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23315
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2212_length_23315_cov_4.980138"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1750
                     /locus_tag="PK710_01735"
     CDS             <1..1750
                     /locus_tag="PK710_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08454.1"
                     /translation="YPGWTATDVVHIGMSKALGTNVLHYVALDLASTLAHEVGDSAAR
                     DRYRGFADDLRDAIRNRLWLEEEGLFSTYVTTWLDPAPVRRFDLLGSALAVLTGIATP
                     SQATRILARYPHYGPGAPVVWPQQQLTPIYHNRGEWPFVTAYWLRAAKVARNDTVADR
                     MIHALLRGAAVNLSNMENFEAATGAAWQEDGSYSGPVVNSERQLWSVAGFLSMVHHTL
                     FGLEAADHGLRVHPFIPATIRSGLLGGTDELVLNDYPYRDKQVTVVMHLPTSGGSGAL
                     QVQSLQLNGQDVTGDLLPDTALAAQNRVDVFLSPGSEPNADCTMISNTDWRNVFGPRT
                     PTIKGIDLVSNGLRLSVDTSEPQDVSLRIYRDGVVVADALPGSTTSWTDTPVPSGTPC
                     YVVEATFVASKNHSQHSAPVCWWGSTGERITDIGASSFVNVGGVASTNHGRFHYETWG
                     DEGHTLTVPSFVPSHSGSHLIQLAYGNGAGPINTGITCAVKQVVVEEAATGSVVGSGI
                     VLMPHLESWDHWAGSSFVPVQLQSAKTYRIIIRGGERAINMSSFAHFEAYTGGLGGES
                     GEFNRVNISHVRILEK"
     gene            complement(1846..3108)
                     /locus_tag="PK710_01740"
     CDS             complement(1846..3108)
                     /locus_tag="PK710_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08455.1"
                     /translation="MGGLRHPGADTFVFWLSTVWVRAANRDLKWKWQRLQYQGCSLAL
                     GGVRMVDRCLVSLAVLIVLIGDLGCQRTGESSNGFIPPDGAMAEAPPSRGGSGGGLQE
                     TGGDAELGGDGGANIEGGFEDVGSDGAVEGGGGHAGGAIEPIDLRQYFPSTTTVVRTR
                     HHDGVLYGTYTYFPASADFVFLYDHFFSLGISGDLFVWGKGYGTEGCTATYAHLFLGD
                     DGSFTEVGDYLATDQCHPSVAFGYGAAQTSANDGLAWAAAGGLPLPGGNGQPAVKEGL
                     RIRAQNQPGAPYLDTPARAWNRTVVVERLPTFRPAHGRSPQGDWGKNLGKTYDDVLRV
                     VFYHGTWQPHQEDIVCPTDDAWAYSGLYEHHAGYHSYASELYLAPGRWIIQETFLFTE
                     SGSYWGMPNCVGLSLDKNPLWTVYIDDP"
     gene            complement(3304..4785)
                     /locus_tag="PK710_01745"
     CDS             complement(3304..4785)
                     /locus_tag="PK710_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT08456.1"
                     /translation="MRPGIRLQLLLALAGMMLLAFVPLYFAVVGLTHSTLQSSWDLAA
                     RSMGRAVAAHVLDARTTRLAHELGPLLQAEIGYGGVTAIAIYDAQGNQLQHAGDTPAA
                     ASLPRTVPGNTERTRTIETAEGRALEVLVPSSDGSVLAIVRTDDETVRAQPLVRLVGY
                     YTAFFALAFLVFTYMALTYVIVRPLDRLVVAASRVSAGARKLEVPRAGAREIAALGAS
                     FSEMTQQLRSEEETLRQKVHELEKTTDDLRSAQRTLVRSERLASVGKLSAGLAHEIGN
                     PIAAILGLLDLMLSEDLSEQEQKDFLLRMRKETERIHETLRQLLDFARPATASHDRTG
                     EEPCFPHQAIEDACALIEPQRAYRNIELVREIHATELVSLARGHLTQVLINLLLNAAD
                     AVAGKGRVWVRLKKELAGACLEVEDDGPGISDEIRDALFEPFVTTKEVGAGTGLGLAV
                     CRGLIESAGGTIHAEDGEYGGARFVVKLPWANPDTDSIGESSR"
     gene            complement(5012..6310)
                     /locus_tag="PK710_01750"
     CDS             complement(5012..6310)
                     /locus_tag="PK710_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT08457.1"
                     /translation="MNKSTRTTPVVLVADDEPSMLALVARHVRTLGYSVLEASDGEAA
                     WREAESSLPDLVVLDVMMPGMSGWEVCRRIRESISLHHTGVIMLTGIGQSLNELTSPL
                     YGADAYIDKPFEFRDLDRKISQVLTERGHEPPVVEQPSLFGSHTSEQEDHSNSDDFED
                     DTDGEESDRAPIEEHGASETRAYDPSMNESAHSWGGQHHASAQKFDHGEPVAAETHFD
                     KTLYASHQPLSVDVIDDNEEDEFSMAVTLRPPPPASSSNHGAWGVDPPAAIETLEEDE
                     EEEPITQRPGPRPSSSEPSTATEMQKASREEKPNNAIPKKSANKAAKIGKTKKVAESK
                     PATKKASDMAPGKDATKKSSKRAACKRATTKEAPAKKATTKKATTKKAPAKKAPAKKA
                     PAKKVANPKTTTKAASKTVAKKTAPKKSTKKASTKKSSSR"
     gene            6768..8687
                     /locus_tag="PK710_01755"
     CDS             6768..8687
                     /locus_tag="PK710_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08458.1"
                     /translation="MNQNQPHALRTIRSKARWTGMRLRFSETLRWVTLTIPVPLLVAI
                     GCLALIKIQRLDWIASRPYLIAIAASWVISVGAAMYPWLRRRPAYEGAIALDRYHGLH
                     DRLTNALYFSSSAFSEPTALMQLAVRDGCAHAKDLRPAKAAPIVWPPGVWLAAALAAV
                     VVVVAALEVRTTRTVAEAKTIDAVALNADDIDYFRETLKELENRNQEPEVQQAIDKFN
                     QLVEDIANQRLERTEAFRRMEALDRELRAGTAADKKALEEGLQRIAEELRRSDLTKQA
                     AEALANQKLEEAEKHLKDLAKRLRENPKSVSKPQLEQLRKALEEASKRNEERLRDLQN
                     RRDELQREMDRLLKNKDKKDAGTDEQEERLLRNKRRELEQLNRQIEQQERASRQLNRL
                     DRELAQAAADLARDLGLSAQDLENGAEDLNRMAREQMTDQEKEELRRRIQQMRELLRQ
                     QGQGGQPRMVRMTRFSQRARGGGQRGQRQSGQGQSGQGQQPGQGQSSGQGQGEEVWVI
                     GPDGQKVLIMKSAGGASQSGSGQSGEQPGGQGEQPGGKSWGTGHDPNLTGKATNPQMG
                     TQDVTAEGLDTGQGPSRAEVIQGAAERGFVGRGYKKVFAEYETVAEKAIEKENVPPGY
                     RFYVQRYFQLIRPRE"
     gene            8747..9757
                     /locus_tag="PK710_01760"
     CDS             8747..9757
                     /locus_tag="PK710_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOT08459.1"
                     /translation="MTDSNDRDQTEQRVEDFRTKIGRLREEVGKVIVGHRDVIDGVMT
                     CMLAGSHALLEGVPGLGKTILVRTLAESLSLHFSRIQFTPDLMPADIIGTTVIDESSS
                     GAKVFEFRRGPVFANIVLADEINRATPKTQSALLEAMQEQRVTVAKTTHKLDLPFFVL
                     ATQNPLEMEGTYPLPEAQLDRFFLKLQVSFPDREQLHAIVDRTTGSHMPTVEPVLDGQ
                     AILQLQQLVREVPVARHVQDYAIRLLQACHPDGDEAPDMVRRFVRFGPSPRGAQATLL
                     AAKIRALFEGRFAASTDDVKAVALPALRHRVLLNFEGEAEGVRPDQVLEHIIKKLPES
                     KA"
     gene            9774..10709
                     /locus_tag="PK710_01765"
     CDS             9774..10709
                     /locus_tag="PK710_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOT08460.1"
                     /translation="MGFRAFIRRVSLPPPSIRGEDDLFDDEFQRKIEYLAIVSRRVFA
                     GRMRAERRTRKTGSGIEFADHRDYVPGDDFRYLDWNVYQRFGRLLLRLFEEEEDLSIY
                     LILDASSSMGFGRNKKIVYGKRLIAALAYIGLGNLDRVALVTTTDRVLGRMPSTRGKQ
                     RIFRVFQFLRETHADGTTDLAQALKVFVSQHKRRGLAVLVSDLYDPAGFENGINTLRF
                     NKFEPFVLHLVDPSDASPPLRGDVRIYDCETGEEREVTVTPRVLQKYRQAYDDYLNEV
                     QAFCTKKQVPYVQANIHVPFEEMVLRFLRRGGIVG"
     gene            10713..12602
                     /locus_tag="PK710_01770"
     CDS             10713..12602
                     /locus_tag="PK710_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT08461.1"
                     /translation="MEFLGLPLTTLLSIGAGAGALVVVAYILRLKRRPVPVHFVKLWE
                     RILREKEATSLFSQLKRILSLLLQLLLLTLLIFALGDPRSAAAIIEGRNLVVLIDASA
                     SMQATDGTPTRFESARQQARDLVRGMGGSDRALIVQMDATVTPLSTMTDEIPVLESAI
                     SSTRVTESRADFPRALRFALDSLVGLSNPEVVVISDGRLGVSQDAISLPLGEVKLSYV
                     KVGQRDINAAITQFSVRRYPLDKSRYEVMIEVSNTSKEPLELELSLYGDDTLVDLTKF
                     RVGPGENLPRFYPNLSGASRSLEARLTLANGERDTLPVDDHAYALLPERRRARIQCVT
                     KGNTYLEAALLLDEYLDVTLVDPASYPVLGQQYDVTIFDNVTPDVAPGSGALFYVNPD
                     GDRSPVKVGKGSLEGVGFDTWDKKSPLLRWTTIQNVNIATARKLEPKQEDKVIGASFD
                     GPLLVMGRRAGKPFVAMGFDVRDSDLPLRISWPLLLLNTINEFVNEDTSYISSYRTGT
                     LWRIPVAVDVSEANLETPWGAKQTIPVLQGHAVYLGQSAGVYRMTAGPPDASVQVAFA
                     ANLSDVQESTIAPLDSLEVAGKVAGPVEGFRVGVRREFWIYLLLGVVALTTIEWITYH
                     RRITV"
     gene            12599..15385
                     /locus_tag="PK710_01775"
     CDS             12599..15385
                     /locus_tag="PK710_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT08462.1"
                     /translation="MRGRLRRILWGLLIVTIGGGLFSAYRQVVLLAPEPTLRWIREGI
                     TYELLAPRMLGFVLLAPLFLFALGRSLADLPWQQRLLSVVLRLAFVALLAVGLSRPAR
                     TASSNKICTVYLVDVSDSVPDEALEDAQKVVQAGIDGRRGDDIVRVVTFARRPVVVSY
                     EPGEVPAITRHDPPGSPARLGAGSDLQSALQHAYGLYPAGYLKRAVLLSDGVQTDGNL
                     LAESNRAKEFGVKLYTIAYRRPVPGEVAVRELTLPDRVKVGEPFEVRASLYATRKTKA
                     KAKLYQGEALNGLDGVKNLELEAGPNDISFKSVVRMAGSVTYALKLEDIEHDRFEENN
                     RYATTVDVPGRPTVLYVEGEPSRANYLSSALSAQQFDVDVRGPSAFPGSLKELERYDF
                     VVLSDTPAEKVSLASQDLIEQYVTVLGGGFLFAGGSAGFGLGGWNHTIIERILPVRMD
                     TEHRRETPSLAMALVIDRSGSMMGLPMEMAKAAAKATVDILASNDLVSVIAFDTQPSR
                     YVKMQAARNRTRIANDIARIQAGGGTEIFSALDAAFQDMTVTRARKKHVILLTDGQAP
                     TTGIRDLVQAMIAESITVTTVGLGEEADEQLLRMIADVGGGRFHKVTDPNALPRIFTR
                     ETEMVARQAAVEEWFPVTQVAPADFLRGLDISTAPLLHGYVATRMKPAPAQLILQSDN
                     AEPILARWRLGTGWSLAWTSDVKNLWAVEWLRWQGYGQFWGQLVREHMRTKHRRELDM
                     QASVVDGQVHAVVDAFSADDRFENGLESTLTLVGPEPDGQKRSVAMTQTAPGRYEAWV
                     ELSKYGSYLLRADHSRLNDKGTPTQVAVSYGHISNPYPREYASFEPDRLTLERAASST
                     GGGSDPAPSLVFDPGDEKVEFHEDLWSRFVLSSVAVFLVDLFLRRVRVFDRKFLPRRK
                     DKSQRTSLLPGSVR"
     gene            complement(15511..18027)
                     /locus_tag="PK710_01780"
     CDS             complement(15511..18027)
                     /locus_tag="PK710_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOT08463.1"
                     /translation="MRTYLLACGATLLLGLGCGSEASHTPSSATCQDGLARLQECIDN
                     YCVDSTSKTCTSLRNAGSGSLRGASTDPCASMNARAVDHLMQASCEDLLKEAGLVLDG
                     KADGQCPPYFPWCEQIDRNATGYSVQVESFEPGQIILDVSIHDLGYTSTLFQGQAFHE
                     LTLDASGRTPDIGKPAIPSIGLMIGVPGGTDTAWVESYEIKETAFLQDLHLAPLQQVV
                     LEDAPPPEFAFDAASYALDAPYPGYQHVVGEIATWRNFRVVRVDTFPLQYNPVHRELE
                     VATRFRITVRFADQHPEPIDTVDYGRESFAAAYDETLVNYYEATDDGQTPPPAPTDER
                     VRYLFVVDDPLLGAIQPLIDQKEIDGVKTDVLLTSSVAEGEGTLAERIKTAIQARYEQ
                     DAIEYVLLVGEPEAIPLYNWNGNQSDSWYGCLAGDDLLPEVAIGRMMGKTPEELTIHV
                     HKTLAHYGQMEQPSEAWRNHVLLVAHEQDYPKKYTECLESIRSAEYRTSKVEFSKLYG
                     AEQATNEQVIELINQGVSIVNYRGHGSETAWHEWNHQDFRVDEAPFTNENKLPVVFSI
                     ACLNSSYQNQQTSMAEHWVLHPSGGAVAVLGATQPSYTIVNHDFNRYLFQALLNEGVS
                     EIGALLHRANAKLFNQYGDDRCAAANMRMYTWLGDPSLRIGEPFEVPPLPPPPGAVII
                     NEVMAHPPSDTNGDGIFDFKSDEFIELVNTGQGDTDISGWTISDKIGVRFTFPQGTIL
                     PGGKALVVFGGGDIEQFADMGGSAVFVASSGLKLNDDGDAVTLSNASSERVDSMEYRA
                     ALARGSSMTRAIDADGATAFEHHPGEPPFSPGKRRDGTPF"
     gene            complement(18089..20287)
                     /locus_tag="PK710_01785"
     CDS             complement(18089..20287)
                     /locus_tag="PK710_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08464.1"
                     /translation="MRSSSFYSGALFSSVAAAIVVAVACGNPEIILPPEQQPSQDAGT
                     DSVPEADSSLLDIPNQEADTKDEAFVIVPADKVIDPVQPGETVQFQAISVKTQEPIEV
                     SFWTVDKMVAGTIDAKGLFTASGVAGGKLEIGAQTAKHQATTSLTLRVRIRDNPGGLD
                     AATQAALEAGQAGAGATNTPDTTFRWLYPYDQTVFPRGLPSPTLQFAGVGADGYYLHV
                     QSEWYEYQGFYSGSSPERLAVPSSVWEGLTWSAGAPDPVSVQMTKIHQGHVTGPVTET
                     WDIAQGSLKGIVYYNTYDDPLATQNGQLDEERRGAVMQIRPGQHAEVLLGGDGTCTVC
                     HSVSANGERMVLSAGHVCNKSFVLSSTGDATEAYSDSCANEGRHDDTFSFGGLTPDGS
                     LMLTSASMKREQPYDFFPNIASVDDDTGPRDSKLVRVDSGQIVAAPGFDGVVRKALMP
                     SFSPNGRKVAFVHYEAGLGNTLSVMDFDPATHTFTNLRVVYDAKKYGFDDLYAGWPAF
                     TPDGKSIVFQVGDRRDYSTWRGGEGQLFIVDVASGNEQPLAWLNGMNSSGSYVGTPQE
                     ARMSFEPTLLPVAVGGYYWVVFTSRRPYGNMIEESGEGPDAPTDQRPSPKKLWVAAIS
                     IGSALGPDPSHPAFYLEGQDLVSGNMRGFWALEPCKQIDQSCESGSECCEGFCRAVEQ
                     DGGTAYVCVPPPTNECVEEYEKCVTTEDCCDADDGYECINGYCARPTPVK"
     gene            20611..21492
                     /locus_tag="PK710_01790"
     CDS             20611..21492
                     /locus_tag="PK710_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOT08465.1"
                     /translation="MHTLLDCLPCFLAQARMVAKTAGISSETEERMVRRTLSHLANAD
                     YRVPSPHIAERLYPLFYEELGDSDPYRLRKQQDNELACALAKTLEPKWKTSSQPLRAL
                     VNLALAANVIDYGVGNAFDLERTIDHVMSQQALVDHIDAFEARLADCRKILYLADNAG
                     EIAFDRELIVHGLGARRVTLVVRGGPIINDVTRKDADQVGLSALVPVIDTGVAVPGVA
                     WERSSREFRNAFEQADLIVSKGQGNFETLEHLHDPRIFFVFMIKCRAVSCLSGYPVHT
                     GVAAFGDRFFLTPSHRS"
     gene            complement(21487..22374)
                     /locus_tag="PK710_01795"
     CDS             complement(21487..22374)
                     /locus_tag="PK710_01795"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HOT08466.1"
                     /translation="MMSKSVSLPFGTALMMAIASPAFGDELPKGLMETKAASDGKTDV
                     ATSGFEAATARDQETKDTTELKISAGGLFSTGNSRAMSLTGAGRFKLRRGDNQFTAAA
                     AGNYAKSSPTADEGMETTLENVQGRARYDRFFTPVLAGFLAASGTRDRFQGLDLRLNI
                     DPGFAFYFIDRKSHQLWTEIGYDLQHDIRRDDAIDAAALEGKLVDKSETRHNARAFVG
                     YDNSINEKVTFNSGLEYIQGLQETTNWRLTWDAGLTSSIGNSFAIATTFTLKYDHNPL
                     PGVEKTDAITAVSVVYTLL"
     gene            22631..>23315
                     /locus_tag="PK710_01800"
     CDS             22631..>23315
                     /locus_tag="PK710_01800"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT08467.1"
                     /translation="MTDFALRFGQQTIPIVGTMLMGRSPTCSLVFTDDSKVSRQHARV
                     FLEGGQLVIEDLGSKNGVFLNGVRIAHRASMRVNDVLNLGNQCLEIVEMKPAPYRNTA
                     TKPEGVVFDEDDEPATETLTGAEPADWHAIEAAWQRADIDLASMLMDQAFEVTALRSM
                     ARGEIDKEDQASMCDLALRMAIETKTGRWLNRLVQVHILVSKPLPRNVTASLPSLVHK
                     VHGLDKSLFE"
BASE COUNT         5021 a         6409 c         6666 g         5219 t
ORIGIN      
        1 ctatccggga tggaccgcaa ccgatgtggt tcatatcggg atgagcaagg cgcttggtac
       61 gaatgttctt cattacgttg cgctcgatct tgcgtccacg ctcgcgcacg aagttgggga
      121 ttcggcggct cgcgatcggt atcggggctt tgcggatgat ctgcgcgacg cgattcgcaa
      181 tcgattgtgg ctcgaggagg aagggctttt ttcgacgtat gtgacgacgt ggctcgaccc
      241 cgctcccgtt cgtcgtttcg atttgcttgg ttctgctctc gccgtactta cgggtattgc
      301 cacgccatct caggcgacgc gaattctcgc tcggtatccg cattatggcc ctggcgcccc
      361 ggtcgtttgg cctcaacaac agcttacgcc catctaccac aaccgtggcg agtggccttt
      421 cgtgacggcc tattggcttc gcgcggccaa ggtagcgcgc aacgacacgg tggccgatcg
      481 gatgatccac gcgctcctgc gaggggctgc cgtcaatcta tccaacatgg aaaacttcga
      541 agccgcgacg ggcgcggcct ggcaagaaga tggaagctat agtgggcctg tcgtcaactc
      601 cgagcgacaa ctctggtctg tcgctggatt cctgtcgatg gtccatcaca ccctattcgg
      661 gctggaagcg gctgaccatg gcctgcgtgt tcatcccttt atccccgcta ccatccgctc
      721 cggattgctg gggggaaccg atgaactggt tctcaatgac tatccgtatc gggataaaca
      781 ggttacggtc gtcatgcact tgccgacgag cgggggtagt ggtgcgctgc aggtgcaatc
      841 ccttcagctc aatggtcaag atgtaacggg tgatttgttg cccgatacag cccttgccgc
      901 gcaaaaccgc gtggatgtat ttctttcccc cggttcagag cccaacgccg attgcacgat
      961 gatatccaac accgattggc gaaacgtatt tggtcctcgc acccccacga tcaagggtat
     1021 cgatctcgtc agtaacgggc ttcgtttgtc agtggatacg agcgagccac aagacgtcag
     1081 cctcaggatt tatcgggatg gagtcgttgt ggccgatgca cttcccggaa gcacgacgag
     1141 ctggacggac acgcctgtcc cgtcgggaac gccttgctat gtcgtggagg cgacgtttgt
     1201 ggctagcaaa aatcattcac aacactccgc tcccgtgtgt tggtggggat cgaccggtga
     1261 acgaataacc gatatcggag cgtcttcctt cgtgaatgtg ggcggagtcg cttcgaccaa
     1321 ccacggccga tttcattatg agacatgggg cgacgaggga catacgctca cggtgccgtc
     1381 gttcgttccc tcccattcgg gctcccatct gattcagctt gcttatggca atggggctgg
     1441 cccgatcaac acgggtatta cctgcgccgt caaacaggtt gtggtggaag aagcggccac
     1501 gggaagtgtc gtgggctcgg gaatcgtgct gatgcctcac ttggagtcat gggatcattg
     1561 ggcaggatcg agctttgttc cggtacaact ccaatcggca aagacctatc gaatcatcat
     1621 ccgtggcggc gagcgggcca tcaatatgtc ttcgtttgct cacttcgaag cgtataccgg
     1681 cggcctggga ggagaatccg gtgagttcaa ccgggtgaat atctcgcatg tgcgaattct
     1741 cgaaaaatga acgcacaccc catccttacg gaaaaccgcg gatagacgat ttcaggtagc
     1801 tatccgtatc cggtctgtta tcgcataggt cggtttctcg aaagatcagg ggtcgtcgat
     1861 gtaaacggtc catagcgggt tcttgtccaa gctcaacccc acacaattcg gcattcccca
     1921 gtagcttccg ctttccgtaa aaagaaacgt ctcttgaatg atccagcgcc ccggcgccag
     1981 atacagttcc gatgcatatg agtggtaacc ggcgtggtgc tcatacaaac ccgaatacgc
     2041 ccaagcgtcg tccgtgggac agacaatatc ctcttggtgg ggttgccacg tgccgtgata
     2101 gaaaacgact cgcagaacat cgtcataggt ttttccaagg ttcttccccc agtccccctg
     2161 gggtgatcgt ccatgagcag ggcggaatgt cggcagtcgc tcgacaacga ccgtgcggtt
     2221 ccaggctcgt gccggagtgt cgagatatgg ggcgcctggt tggttctgcg cgcggatacg
     2281 caatccttct ttgacggccg gttgcccatt acccccgggt aagggcaatc ccccggctgc
     2341 tgcccaggct agaccgtcgt tggccgacgt ctgtgcggca ccgtacccaa atgccaccga
     2401 aggatgacat tggtcggtcg ctagataatc tccgacctcc gtgaagctcc catcatcccc
     2461 caaaaacaaa tgggcatagg ttgccgtaca accttcggtg ccatagccct tgccccacac
     2521 gaataagtca ccagaaatcc ccaaagaaaa gaaatggtca tacagaaaga cgaagtccgc
     2581 ggaagccggg aagtaagtat acgtaccgta caacacgccg tcgtgatgcc gagtccgcac
     2641 aactgtcgtg gtggagggga agtactgccg caaatcgatc ggctcgatcg cgccacccgc
     2701 gtgtccgccc ccaccctcga ccgcgccatc ggagccgacg tcttcgaagc caccctcgat
     2761 attcgcaccg ccgtcgcctc ccaactcagc gtcgcccccc gtttcttgaa ggccgccacc
     2821 ggaccctccc cgcgaaggtg gcgcctcggc catggctcca tccggtggta tgaaaccatt
     2881 cgaggattct cccgtgcgct ggcaccccag atcaccaata agcacgatga ggacagccag
     2941 cgacaccaga caacggtcaa ccattcgtac tcccccaaga gccaacgagc acccctgata
     3001 ttgtaggcgt tgccatttcc acttcaagtc acggttggcc gctcgaaccc atacggtcga
     3061 aagccaaaac acaaaggtgt cggcgccggg gtgtcgcaag ccacccacgg tgtcgagaat
     3121 tcgtataacc tactgatatt atatgtatta tttgcacgcc tcgaacgtac gacctcatcc
     3181 ggcgttagcc gtcgttttca ccaacgttcg atcgagacgc gacccaagac cgaccttgaa
     3241 aattctttgc agccacgggt ccttgcgacg gcgttccaca aacccttcga tgtctccacc
     3301 gcatcaccgg gacgattccc cgatgctatc cgtatcggga ttagcccagg gtaatttgac
     3361 gacgaatcga gcgcctccat attcgccatc ttccgcgtgt atcgtccccc ccgcactttc
     3421 tatcaaccct ctgcatacgg ccagaccgag tcctgtaccc gctcctactt ccttggtggt
     3481 tacgaacggc tcgaacaacg catcccggat ctcatccgaa atgccagggc catcatcctc
     3541 cacctcgaga caagcgcctg ccaattcctt tttcaatcga acccagacgc gccctttgcc
     3601 cgccaccgca tcggccgcat tgagcaacaa gttgataagc acttgcgtga ggtgtcctcg
     3661 cgccagagaa acgagttccg tggcgtgtat ttctcgcacg agttcgatgt ttcggtaggc
     3721 acgctgcggt tcgatgagcg cacacgcgtc ttctatcgcc tgatggggga aacatggttc
     3781 ctcacccgtc cgatcgtggg acgccgtcgc cggtcgtgca aagtccagca gttgcctcaa
     3841 ggtttcatga attctttccg tttcctttcg catgcggagc agaaaatctt tctgttcctg
     3901 ttcggaaagg tcctcggaca acatcaggtc cagaagtccg aggatggctg cgatcgggtt
     3961 gcctatttca tgcgccagtc ccgcggacag cttgcccacg gaagccaatc gctccgaacg
     4021 aacgagcgta cgttgcgcgc ttcgaagatc gtcggtggtt ttttccagct catgaacctt
     4081 ctgccggagg gtctcttctt ccgagcggag ttgctgcgtc atctcagaaa aactcgcgcc
     4141 aagggcagcg atttcacggg cgcccgcgcg aggaacctcg agtttgcgtg cgcccgccga
     4201 cactcgactc gccgccacca ccaaccggtc gagaggacgt acgatcacat acgtcaaggc
     4261 catgtacgtg aacacgagaa acgccagcgc aaaaaaagcg gtgtagtagc caaccaatcg
     4321 gacgaggggc tgtgcacgaa cggtctcgtc atccgtacgg acgatcgcca gcactgatcc
     4381 atcgctcgag ggaacgagca cctccaacgc gcgtccttcc gccgtttcga tggtgcgcgt
     4441 acgttcggtg ttcccgggca cggttcgtgg tagcgacgcg gcggccggag tatcgccagc
     4501 atgctgaagc tgattgcctt gcgcatcgta aatggcgatg gccgtcacgc caccgtaccc
     4561 aatctcggcc tgcaagagcg ggccgagttc atgggccaat cgcgtggtcc gtgcgtccaa
     4621 tacatgcgcg gcaacggcgc ggcccatcga tcgtgccgcc aagtcccagg aagactgaag
     4681 cgtagagtga gtaagcccaa ccacggcaaa ataaagcgga acgaatgcga gcaacatcat
     4741 gcccgccaga gcaagcagaa gctgcaaccg aattcccggt ctcaaggtcg agcctttcgc
     4801 aactcgcatc gctcaacata atatcctgca tttttcgttg gttgagacaa cacgactcca
     4861 tgagggaaac catcttcgcc attggcttcg tcgaggatat ccaagacgat gcgaagcctt
     4921 ttcatcacct gtagaaagtg agtgtcgaac gaagggcaga taggcagggt cgtttcgaca
     4981 agaagcggta gggactgcga gccacaacca atcatcgact ggatgatttt ttggtcgagg
     5041 ctttcttcgt cgatttcttg ggagcggtct tctttgccac agtttttgat gcggccttgg
     5101 ttgtcgtctt tgggttcgcc accttcttgg ctggtgcctt cttggctggc gctttcttgg
     5161 ctggcgcttt cttggtcgtc gctttcttgg tcgtcgcttt cttggctggc gcttctttgg
     5221 tcgtcgccct tttgcatgcg gctctcttcg acgatttttt tgtcgcgtct ttccccggag
     5281 ccatatcgct agctttttta gtcgctggtt tactttcggc tactttcttc gttttgccaa
     5341 ttttcgcagc cttatttgcg gattttttcg gtatggcgtt gttgggtttt tcctcgcgcg
     5401 acgccttttg catctcggtt gccgtcgatg gctccgatga ggaaggacgc ggtcccggtc
     5461 gctgtgtgat cggttcttct tcctcatctt cttccaacgt ttcgatggca gcaggtggat
     5521 ccacccccca cgccccgtgg tttgacgatg atgccggtgg tggaggacgt agcgtgaccg
     5581 ccatggaaaa ctcgtcttcc tcgttgtcgt cgatgacatc tacggaaagc ggttggtgtg
     5641 aagcataaag cgttttatcg aagtgcgttt ccgcggccac cggctcacca tggtcgaact
     5701 tctgagcaga tgcgtgatgc tgtccgcccc aactgtgggc cgactcattc atcgacggat
     5761 cgtacgcgcg tgtttccgat gcgccatgct cctcgattgg cgcccgatcg gattcctcgc
     5821 catctgtgtc gtcctcgaag tcatccgagt tggagtggtc ctcttgctcc gaggtgtggg
     5881 aaccgaaaag cgacggttgt tctacgacgg gcggttcatg acctctttcc gtcagaacct
     5941 gagaaatttt gcggtcgaga tctcgaaact cgaaaggttt gtcgatgtag gcatcagcgc
     6001 catagagcgg agatgtaagc tcgttgagac tttgtccgat gcccgtgagc atgatgacac
     6061 cggtatgatg caagctaatg ctctcacgaa tgcgtcggca gacttcccaa ccgctcatgc
     6121 cgggcatcat gacatcgagc acaaccaagt cgggaaggct gctttccgct tctctccaag
     6181 ccgcttctcc atcggacgcc tcgaggactg aatagccgag ggtacgcaca tgtcgagcca
     6241 ccaatgccag catgctgggt tcatcatcgg cgaccaagac aacgggagtc gtccgcgtgc
     6301 tcttgttcac ttcgccatct ccttgcttgt cgacacccac ggtaaccaat gcgggcactg
     6361 ctctcgcctg ggcgtgcgtg tgcaaaacgt catggggtga attggagtac ggcaactcgg
     6421 gttaggtcaa gcatgcgctt ccgtgagagg cgaaataacg tacggatttc ggagcgccca
     6481 cctcgaaacc tcaaaaatcc acccaaatac ggatgaattg ggccataacg cccaagaaac
     6541 gggaacaatc caagcgtttg gggcgtcatg ataaaacagc ccacgccgga tctgcctcgg
     6601 gataaaggac ttaactattg aaatataaac gatattttat ttttcgccgt ttttctgaga
     6661 cccgggcgaa ggccgaaaac gtcgctcccc ttctgcccca aggctcgaag ttctggcgcg
     6721 tctgaccaat caatgatgct gtattcgaac ccgatgtgag ttctgtcgtg aatcagaatc
     6781 agccccacgc ccttcgcacg attcgatcca aagctcgctg gacggggatg cgtttgcgtt
     6841 tctccgagac tctgcgatgg gtcaccctca ctatccccgt tcccttgttg gtcgcgatag
     6901 gctgcctggc tctgatcaag atccaacgac tggattggat cgcttctcgt ccttatctca
     6961 tcgcgatagc ggcctcttgg gtgattagcg ttggggcggc catgtatcca tggttgcggc
     7021 gtcgtccggc gtacgaagga gcgattgctc tggaccgcta tcacgggctc catgatcgct
     7081 tgacgaatgc gctgtacttt tcttcgtcgg cgttttcgga acccacggcc ttgatgcaac
     7141 ttgccgttcg agacggctgt gcgcacgcga aagatcttcg tccggcgaag gcggcgccga
     7201 ttgtctggcc gcccggagta tggcttgccg cggcgctagc tgccgtggtc gttgtggtag
     7261 cggcgctcga ggttcgaacg acacggaccg ttgctgaggc gaaaacgatc gatgcggtcg
     7321 cgttgaatgc cgacgatatc gattatttcc gcgagacgct caaggagttg gaaaaccgca
     7381 accaagaacc ggaagtgcaa caagccatcg ataaattcaa tcaactcgtc gaggatatcg
     7441 cgaaccaacg gcttgagaga acggaagcat ttcgacggat ggaggcgctg gaccgggagc
     7501 ttcgagctgg gacggcagcg gataaaaaag ctttggaaga aggtctccaa cggatagctg
     7561 aggaactacg tcgaagcgat ttgaccaagc aagccgctga agctcttgcc aatcaaaagc
     7621 tcgaggaagc ggaaaaacac ctgaaggatc tcgccaaacg cttacgagaa aacccgaaat
     7681 ccgtgtccaa accgcagctc gaacaacttc gaaaagctct cgaggaagcg tcgaaacgaa
     7741 acgaggagcg tctgcgggat cttcaaaacc ggcgtgacga actgcaacgc gaaatggatc
     7801 gtttgttgaa aaacaaagac aaaaaggacg caggaacgga cgaacaagag gagcgtttgc
     7861 tgcgcaacaa gcgaagggaa ctcgagcaac tcaatcgaca gatcgaacag caagagcggg
     7921 caagtcgtca gttgaatcga ttggatcggg aacttgcgca agcggcagcg gatctggctc
     7981 gtgatttggg gttgagtgcg caggacttgg aaaacggcgc cgaagacttg aatcgaatgg
     8041 ctcgcgaaca gatgacggat caagaaaaag aggagcttcg tcggcgtatc caacaaatgc
     8101 gagaactgtt gcgtcagcaa gggcaagggg ggcagccgcg gatggttcga atgacacggt
     8161 tttcacagcg agcacggggg ggaggccagc gagggcaacg acaatccgga cagggacaat
     8221 ccggccaggg gcaacaaccg ggtcaagggc aatcatccgg tcaagggcaa ggcgaggaag
     8281 tatgggtaat tggccctgat ggacagaaag ttctgattat gaaatccgcg gggggggcca
     8341 gccaatccgg aagtggacag tccggggaac aacctggtgg tcaaggtgaa cagcccggcg
     8401 gcaagtcatg gggcacgggg cacgatccga atcttacggg caaagccacg aatccccaga
     8461 tgggtacgca agacgtaacc gcggaaggac tcgacaccgg tcaaggaccc tcccgtgcgg
     8521 aggtgattca gggagctgca gaacgaggct ttgtggggcg agggtacaag aaagtatttg
     8581 cggaatacga aaccgtagcg gaaaaagcga tcgaaaaaga aaacgttccc cccggctacc
     8641 gtttctacgt ccagcggtac tttcagctca ttcgaccacg tgaatagggt gtggcacgac
     8701 gagtccggct tgcccatctt gctttcgact caaccgaggt aaccgtatga cggattcgaa
     8761 cgatagggat caaacagaac aacgtgtcga ggatttccga accaaaatcg gccgtcttcg
     8821 cgaagaggtt ggcaaagtca tcgtgggtca tcgtgatgtg atcgacgggg tgatgacgtg
     8881 catgctcgcg gggtcccacg cgttgttgga aggagttccg ggcctgggaa aaacgatttt
     8941 ggtgcggacg cttgcagaat cgctttcgct gcatttttcc cgtattcaat tcacgccgga
     9001 tctgatgccc gcggacatca ttggcaccac cgtcatcgat gagtcctctt ccggtgccaa
     9061 ggtctttgag tttcgacggg gacctgtttt cgccaatatc gtgttggccg acgagatcaa
     9121 tcgggccacg cccaaaacac aaagcgcact gctcgaagcc atgcaggagc agcgtgtgac
     9181 ggtggcgaaa acgacccaca agctcgatct gccgtttttc gttcttgcca cacaaaaccc
     9241 gctcgagatg gagggaacgt acccgttgcc cgaagcgcag ctcgaccggt tttttctcaa
     9301 gctgcaggtc tcctttccag accgcgaaca actgcatgcc atcgtggacc gcaccaccgg
     9361 cagccacatg cccaccgtcg aacccgtgtt ggatggacag gccatcttgc aattgcaaca
     9421 gctcgtgcgg gaggtgcctg tcgcacggca tgtgcaggac tacgccattc gattgcttca
     9481 agcctgtcac cccgatggcg atgaagcgcc cgatatggta cgtcggttcg ttcgcttcgg
     9541 accatcgccc cgtggcgcgc aagccacctt gctggccgca aaaatacgcg cgctgttcga
     9601 agggcgtttc gccgcctcca ccgacgacgt gaaggcggtt gctttgccgg cgttgcggca
     9661 tcgtgtcctg ctcaacttcg aaggggaagc agaaggagtg agacccgacc aggtcctcga
     9721 acacatcatc aaaaaacttc cggaaagcaa ggcctgagcc tggagacacc gccgtgggct
     9781 ttcgcgcttt cattcgtcgc gtctctcttc ctccaccgtc cattcgcggt gaggacgacc
     9841 tatttgacga cgagtttcaa cgaaagatcg aataccttgc gatcgtaagt cgtcgtgtgt
     9901 tcgcgggacg gatgcgcgcc gaacgaagaa cgcgcaagac tggttctgga atcgagttcg
     9961 cggatcatcg agactacgtg ccgggggatg atttccggta tctcgattgg aacgtgtatc
    10021 aacgtttcgg aaggctattg cttcgtttgt tcgaagaaga agaggacttg agtatttatt
    10081 tgatcctcga cgcttcgagt tcgatggggt ttggacgaaa taaaaaaatt gtgtacggca
    10141 agcgactgat tgcggcattg gcgtatatcg gtctgggcaa tctggaccgc gtcgctctcg
    10201 tcaccaccac ggatcgagtg ctgggaagaa tgccttccac tcgtggaaaa caacgtattt
    10261 tcagggtgtt tcagttctta cgggagacgc atgcggacgg aacgacggat ctcgcgcaag
    10321 cgttgaaggt atttgtcagc cagcacaagc gtcgtgggct cgcggtattg gtcagcgact
    10381 tgtatgaccc cgctggattc gaaaatggga tcaacacgct caggttcaac aagttcgaac
    10441 cctttgtatt gcatttggtg gatccttcgg atgcaagtcc accgttgcgg ggtgacgtgc
    10501 ggatttatga ctgtgaaaca ggagaagaac gagaagtgac ggtaacaccg cgtgtgttac
    10561 agaagtaccg ccaagcctac gatgattact tgaacgaggt gcaagcgttc tgcacgaaaa
    10621 aacaggtgcc ttacgtacag gcgaacattc atgtgccgtt cgaggaaatg gtgctgcgct
    10681 ttcttcgtcg cggggggatt gtggggtaag gcatggagtt tcttggcctt ccgctcacta
    10741 cgcttttgtc gatcggcgcc ggcgcgggtg ctttggtggt ggtggcctat attttacggc
    10801 tgaaacgccg ccccgtaccg gttcatttcg tcaaactatg ggaacggatt ctgcgagaaa
    10861 aagaggccac gagcctgttt tctcagctca agcggattct gtcgctgctt cttcagcttc
    10921 tgctactcac cctgctgatt ttcgcgctcg gcgacccacg cagcgccgct gccatcatcg
    10981 aaggtcggaa cctggtggtg ctcatcgatg caagcgcatc gatgcaagcg acagatggca
    11041 cgccgactcg cttcgaatcc gcacggcaac aagcgcgcga tctggttcgc ggcatgggcg
    11101 gatccgaccg tgccttgatc gttcaaatgg atgccacggt tacgccgctg tcgaccatga
    11161 ccgacgaaat ccctgtactg gaaagcgcca tctcttctac ccgcgttacg gagtcccggg
    11221 cggactttcc acgcgctctt cgattcgctc tggactctct cgtgggattg tcaaacccag
    11281 aagtcgtcgt catttccgac gggcgactgg gtgtatctca agacgccatc agccttccgc
    11341 tcggtgaggt caagctgtct tatgtcaaag ttgggcaacg ggatatcaat gccgcgatta
    11401 cccaattttc agtgcgacgt tacccgctcg ataagtcccg ttatgaggtc atgatcgagg
    11461 tgtcgaatac ctcgaaagaa cctttggaac tcgagctatc cctctatggt gatgacacgc
    11521 tcgtcgatct gaccaagttc cgcgtgggtc caggagaaaa tctacctcga ttctatccga
    11581 atttgtccgg tgcaagccgt tcgctcgaag cgcgtcttac ccttgccaac ggcgaacggg
    11641 acacgttgcc cgtggatgat catgcgtacg cgctgttgcc ggagaggcgt cgggcacgga
    11701 tacagtgtgt caccaaagga aatacgtatc tggaagcggc cctgttgctg gatgagtatc
    11761 tggatgtgac gttggtggat ccagcctcgt atcccgtttt gggtcagcaa tatgacgtga
    11821 ccattttcga taatgtgaca cccgatgttg cgcctggatc gggggccctg ttttatgtca
    11881 atccggatgg ggatagaagc cccgtgaaag tgggaaaggg ctcgctcgaa ggcgtggggt
    11941 tcgatacgtg ggacaagaag agtccgttgc ttcgatggac gacgattcaa aatgtcaata
    12001 tcgctaccgc ccgcaaactc gaacccaagc aggaagacaa ggtcatcgga gcgtctttcg
    12061 atggaccgtt gctcgtgatg ggacgtcgcg cgggaaaacc gttcgtagcg atgggattcg
    12121 acgtcaggga tagcgatctg ccccttcgca tttcatggcc tttgttgttg ctcaacacca
    12181 tcaacgaatt cgtcaacgag gatacttcgt atatctcgtc gtatcgaacc ggtacgcttt
    12241 ggcgtattcc ggtggccgta gatgtcagcg aagccaatct ggaaacgccg tggggtgcga
    12301 aacaaaccat acccgtccta caaggacatg cggtttacct cggtcaatcc gcgggcgtat
    12361 atcggatgac cgcgggcccg ccggatgctt cggtgcaagt ggccttcgca gcgaaccttt
    12421 cggatgtgca agagtccacc atcgctcctt tggatagttt ggaagtggca gggaaggtag
    12481 cggggcccgt cgaaggtttt cgagtcggag tacgaagaga attctggatt tatttgctgc
    12541 ttggggtggt ggctcttacg acgattgagt ggattaccta tcaccggagg atcacggtat
    12601 gagaggtcga ctcaggcgga tcctgtgggg attgttgatc gtgacgatcg gaggggggct
    12661 gttttccgcg tatcgccagg ttgtgttgtt ggctccggaa cccacgttgc gttggattcg
    12721 ggagggaatc acgtacgagc ttctcgcgcc ccgcatgctt gggttcgtgc tgttggcgcc
    12781 gctatttttg ttcgctttgg gacgtagtct tgccgatctt ccctggcaac aacgtctcct
    12841 gtcggttgtg cttcgtctcg cgttcgtagc gttgctcgcg gtgggtctgt ctcgtcccgc
    12901 gcgaaccgcc agcagcaaca agatctgtac ggtatacctt gtcgacgttt cggattcggt
    12961 gccggacgag gctctcgagg acgcgcaaaa agtggttcaa gcggggatcg atggccggcg
    13021 aggcgacgac atcgttcggg tggttacgtt tgcccgtcgg ccggtggttg tttcgtacga
    13081 acccggagag gtgccggcga tcacgcggca tgatccccct ggttcgcctg cccgtttggg
    13141 ggctggctcc gaccttcagt cggctcttca acatgcctat ggattgtatc ccgcggggta
    13201 tttgaaacgt gcggtcctgt tgtccgatgg cgttcaaacc gatggcaatt tgctggcgga
    13261 atccaatcgc gccaaagaat tcggggtcaa gctttatacc atcgcctatc gaagaccggt
    13321 tcctggtgaa gtcgccgtgc gtgagttgac gcttcccgat cgagtcaaag tcggcgaacc
    13381 cttcgaagtc cgcgcttcgc tgtacgccac gagaaaaaca aaagccaagg cgaaactgta
    13441 tcagggagaa gccctcaacg gactcgacgg cgtaaaaaat ctcgaactcg aagccggtcc
    13501 caacgatatc tccttcaagt cggtcgtgcg aatggcggga agcgtcacct atgcgctcaa
    13561 actcgaagac atcgagcatg accgtttcga agaaaacaat cgctacgcaa ctaccgtgga
    13621 cgtgcctggt cgccccacgg tcttgtacgt ggaaggggaa ccgtcgcgcg ccaactacct
    13681 gtcgagcgcg ttgagcgcac agcaattcga tgtggatgtg cgtgggccgt cggccttccc
    13741 cggctccttg aaagaattgg agcgatacga tttcgtggtg ctgtcggaca ccccggcgga
    13801 aaaagtctcc cttgcctcgc aggatctcat cgaacagtac gtaacggttc ttggcggtgg
    13861 attcttgttc gctggaggaa gcgcgggctt cggattgggt ggatggaatc acacgatcat
    13921 cgaacgcatt cttcccgttc gcatggacac cgaacaccgt cgagaaacac catcgctcgc
    13981 catggcattg gtcatcgatc gatctggctc catgatgggg ttgcccatgg agatggccaa
    14041 ggcggcggca aaggcaacag tcgatatcct tgcgagcaac gatctggtgt cggtcattgc
    14101 ctttgatacg cagccttcgc gatatgtgaa aatgcaagcg gcgcgcaatc gaacgcgtat
    14161 agccaacgac attgcccgca tacaagcagg aggtgggacg gagattttct cggcattgga
    14221 tgcggctttt caagacatga ccgtgacgcg agcacggaaa aagcatgtga ttttgttgac
    14281 cgatggtcag gctccgacca ccggtatccg cgatctggtg caagcgatga ttgccgagtc
    14341 catcacggtc accaccgtgg ggcttgggga agaagcggac gagcaattgt tgcgaatgat
    14401 cgcggatgta gggggaggtc gattccacaa ggtgacggat ccgaacgcgc taccccgcat
    14461 ctttacgcgg gaaacggaga tggtggctcg ccaggccgcc gtcgaagaat ggtttccggt
    14521 cacgcaagtc gcccccgcgg attttctgcg cggtctggat atcagcacgg cgcctttgct
    14581 gcacggatac gtggcaacac gcatgaaacc cgcgcccgca caactcatcc tgcaaagtga
    14641 caacgccgag ccgatcttgg cgcgatggcg tctcggcacc ggctggtccc ttgcgtggac
    14701 cagcgacgtc aaaaaccttt gggccgtcga atggctacga tggcaaggct atggtcaatt
    14761 ctggggacaa ctcgtgcggg aacacatgag aaccaaacat cgccgcgagc tagacatgca
    14821 agcttccgtc gtcgatggcc aggttcacgc cgtggtcgat gcgttcagcg ccgatgatcg
    14881 attcgaaaat gggttggaat cgacgttgac cctcgttgga ccggaaccgg atggacaaaa
    14941 acgttcggtt gccatgaccc agacagcgcc agggagatac gaagcctggg tggaactgtc
    15001 caagtatggc tcctatttgc tgcgagcgga ccattcccgt ttgaacgata aaggaacgcc
    15061 tacccaagtt gccgtttcct acggccatat ttccaatccg tatccgaggg aatacgcaag
    15121 tttcgaaccc gatcgattga ccttggagcg ggcggcaagc tcgacgggag gaggaagcga
    15181 tccggctccc tctttggttt tcgatcctgg agacgaaaaa gtcgagtttc atgaagacct
    15241 gtggtcgcgt ttcgttcttt cctccgtggc cgtgttcttg gtcgatctat tcttacggcg
    15301 agtacgggtt ttcgatcgga aattcttgcc gagacgaaaa gacaagtcgc aacgtacttc
    15361 gttgcttccc gggtcggtcc gatgagcttg tgcttggttg attatccata aacggatagc
    15421 aacgcatcga tggcgaaggg atgcttcagg cgtgatggga atacgggttg gatttcgggg
    15481 gcgccgcgat gggttgctgc ccgaacgatc tcaaaacggg gtgccgtctc gtcgcttgcc
    15541 gggagaaaaa ggtggttcgc cgggatgatg ctcgaacgcc gtggcgccgt cggcatcgat
    15601 cgccctggtc atcgatgaac cccgggccaa ggccgctcga tattccatgg aatcgacgcg
    15661 ctccgaggag gcgttggaca acgtgaccgc atcgccgtca tcgttgagtt tgaggcccga
    15721 tgaggcaacg aatacggcgc tgccgcccat gtcggcgaat tgttcgatat caccgccgcc
    15781 aaatacgacg agtgccttgc ctccgggaag gatcgttcct tgcgggaagg tgaaacgcac
    15841 gccgatttta tcggagatgg tccaaccgga aatatccgtg tctccctgcc ccgtgttgac
    15901 gagttcgatg aactcgtcag acttgaaatc aaagatacca tcgccgttgg tatcactggg
    15961 aggatgcgcc atgacctcgt tgatgatgac ggcacctggg ggcggcggaa ggggtggaac
    16021 ctcgaaaggt tctccgattc tcaaggaagg atctcccagc cacgtataca ttcgcatatt
    16081 ggcggccgcg catcgatcat ctccatattg attgaagagc ttggcgttgg cacggtgcaa
    16141 cagcgcgccg atctcactca ccccttcatt gagcagcgcc tggaacagat agcgattgaa
    16201 gtcgtgattg acgatcgtat aggacggttg ggtggcgccg agtacagcga ccgctcctcc
    16261 cgaggggtgc agcacccaat gctcggccat cgaagtctgc tgattctgat acgaggagtt
    16321 caagcaggcg atgctgaata cgaccggcag cttgttttcg ttggtgaagg gagcctcatc
    16381 gacgcggaag tcctggtgat tccattcatg ccaagccgtt tcggagccgt gaccgcgata
    16441 attgacgatg ctgacgccct ggttgattag ctcgatgact tgctcattgg tggcttgctc
    16501 cgcgccatag agtttcgaaa actccacctt gctcgtgcga tactccgccg aacgaatgga
    16561 ctcgaggcat tcggtatact ttttcggata gtcttgttca tgggctacga gcaaaacgtg
    16621 attacgccat gcttcggacg gctgttccat ctgcccataa tgggccaacg ttttgtggac
    16681 gtgaatggtc agctcttccg gggtcttgcc catcattcgg ccaatggcaa cctcaggcaa
    16741 taagtcgtcg cccgccaaac aaccgtacca agaatcactc tggttgccgt tccagttgta
    16801 caagggaatt gcttccggct cgcccaccaa aagcacatac tcgatagcat cttgttcata
    16861 tcgcgcctga atagcagtct tgatgcgctc cgcaagcgtt ccctcgcctt ccgccacgct
    16921 gctcgtcaac agcacgtccg tcttcacccc atcaatttct ttctggtcaa tgagcggctg
    16981 aatggcgcca agcaaaggat catctacgac aaacaggtat cgtacgcgct catccgtggg
    17041 agcaggcgga ggcgtctgcc catcatccgt cgcttcgtaa tagttgacga gcgtttcgtc
    17101 atacgctgcg gcaaatgact cgcgaccata atcgacggta tcgattggct ccggatgttg
    17161 gtccgcgaac cgaacggtga tacgaaagcg ggtcgcgact tcgagttcgc gatgcacggg
    17221 gttgtattga agcggaaagg tgtccacgcg cacgacacgg aagttgcgcc acgtggcgat
    17281 ttctcccacc acgtgctggt agccaggata aggggcatcg agcgcgtaag aagcagcatc
    17341 gaaagcaaac tccggcggcg gagcatcttc caaaaccacc tgctgcaacg gcgccaaatg
    17401 aagatcctgc aagaaggcag tctcctttat ctcgtaactt tcaacccacg cggtatccgt
    17461 tcctccgggc acgcctatca tgaggccaat cgatgggatg gccggcttgc caatgtccgg
    17521 tgtgcgtccc gatgcatcca gggtcaattc atgaaaagct tgtccctgaa acaatgtgct
    17581 cgtgtatccc agatcgtgga tcgatacgtc gaggatgatc tgcccgggct caaaagactc
    17641 gacctgaacg ctgtaaccag tcgcatttcg atcgatttgt tcacaccacg gaaaatacgg
    17701 agggcattgc ccatccgctt tcccatcgag aacaagaccc gcttctttga gcaagtcctc
    17761 acaactcgcc tgcatcaaat gatcgacagc cctcgcattc atgcttgcac agggatccgt
    17821 cgaggcaccg cgcaacgaac cagacccggc gttgcgaagc gatgtgcagg ttttcgaagt
    17881 gctgtcgaca caataattgt cgatgcactc ctgaagacga gccaatccat cctgacacgt
    17941 tgcggaagac ggcgtgtgcg aagcctcact accgcaaccc aagcccaaca ataacgttgc
    18001 cccacaggct agaaggtagg tgcgcattgc ggcagcatga atcagaaaac tgacgtttcc
    18061 aagccgtgat ttgtaaattt tttcgatatt acttcacggg agtcggacga gcgcagtacc
    18121 cattgataca ttcatagccg tcgtcggcat cgcagcaatc ctccgtagtt acgcattttt
    18181 cgtattcttc cacgcattcg ttggtgggag gaggaacgca gacatacgcc gtgccgccgt
    18241 cttgctcgac cgcacgacag aagccttcac agcattcgct acctgattcg cagctttggt
    18301 cgatttgttt gcaaggttcc agggcccaaa acccccgcat attgcccgac acgaggtcct
    18361 gcccttccag gtaaaacgct ggatggctgg ggtccggtcc gagggccgaa ccgatactga
    18421 tggccgcaac ccagagtttt ttcggcgaag gtctttgatc cgtgggagca tcggggccct
    18481 cgccagactc ctcgatcatg ttgccgtagg ggcgacgact cgtgaagacc acccaatagt
    18541 atccgccgac cgcgaccggc aacaacgtgg gctcgaagct catccgagcc tcttgtggag
    18601 tgcccacgta cgagccggaa ctattcattc cgttgagcca tgcgaggggc tgctcgttgc
    18661 ccgaagcgac atctacgatg aatagctgcc cctcgccgcc acgccaggtg gaatagtccc
    18721 gtcgatcgcc cacttgaaat acgatggatt tgccgtccgg agtgaacgcg ggccaccccg
    18781 cgtatagatc gtcgaacccg tattttttcg cgtcataaac gacacgaagg ttggtgaagg
    18841 tatgtgtggc tgggtcgaag tccatcacgc tcaacgtatt gccgaggcct gcttcataat
    18901 ggacgaaagc gaccttccgg ccattggggg agaaagaggg catgagggct tttcgtacaa
    18961 cgccatcgaa tcccggcgca gccacgatct gtccggaatc aacgcgaacc agtttcgaat
    19021 cacgaggccc cgtatcatcg tcgacactcg cgatattggg gaaaaagtca taaggctgtt
    19081 cgcgtttcat cgatgcggag gtgagcatca acgaaccatc tggagtcagt ccgccgaaag
    19141 aaaaggtgtc atcgtgacgt ccttcgtttg cgcagctatc ggaatacgct tcggtggcgt
    19201 ctcccgtcga agacaagacg aaggatttgt tgcagacgtg tccggcagac agcaccatgc
    19261 gttcaccgtt tgcgctgaca gagtggcaga cggtgcaggt tccgtcgccg ccgagcagca
    19321 cttcggcgtg ctggccgggt cgaatttgca tgaccgcgcc gcgacgttct tcatcgagtt
    19381 gaccgttttg ggtcgcgagc gggtcgtcat acgtgttgta gtagacgatt cctttgaggc
    19441 ttccctgcgc aatatcccag gtttccgtaa ccggtccggt gacatggcct tgatggattt
    19501 tcgtcatctg cacgctcacc ggatcgggag ccccagcgct ccaggtcaat ccttcccaca
    19561 ccgaagaggg aaccgcgagc cgctctggcg aagaaccgga ataaaaccct tggtattcat
    19621 accattcgga ttgcacgtgc aggtaatagc cgtcggcccc tactccagcg aactgcaagg
    19681 tgggggaagg caagccccgc ggaaaaacgg tttggtcata gggatagagc caacggaacg
    19741 tcgtgtccgg ggtattcgta gctcctgcgc ccgcttgacc ggcctcgagg gcagcttggg
    19801 tcgccgcatc caatcccccc ggattgtccc gaatacgaac tcgtaacgtc agggaagtcg
    19861 tcgcctgatg cttggccgtc tgtgccccaa tctcgagctt ccccccagcc actccactgg
    19921 ctgtgaacaa ccctttcgcg tcgatcgttc ccgcgaccat cttgtcgacc gtccagaaag
    19981 aaacctcgat cggctcctgg gtcttcacag aaatagcctg aaattgaact gtttccccgg
    20041 gttggacggg atcgatcacc ttgtccgcgg ggacgatcac gaacgcttca tccttggtat
    20101 ctgcctcttg gttcgggata tccaacaagg atgaatcagc ctccggcaca gagtccgttc
    20161 ctgcatcctg ggatggttgc tgctcgggcg gaagaatgat ctcgggattc ccgcatgcca
    20221 ctgccacgac aatcgcggcc gccaccgagg aaaaaagagc gccagagtag aaagaagaag
    20281 aacgcatggc gaattcctat tcgaaagact cggattcgaa aagatacata tcaggtccaa
    20341 aggctcgagg ctcatgggat cgtcaataca aagcttcgaa aatagagcgt gtgctgacaa
    20401 tttcgtttcg aaaaaaaagc ggaatagcaa acggcgttgg cggctcatcc ccatacgcct
    20461 agccatgggt cgcagccgga tgacctccaa cggttctcgc ctcgtggcca tgcagttgta
    20521 ttttcgccgc gcaggcgtcg tgtgcgacgc tcgcgtcgac tgccgcggcc acgattggca
    20581 gaaccttcag attaaggata acaaggaccg atgcatacgc tgttggattg tctcccttgc
    20641 tttctcgctc aggcccgcat ggttgcgaaa acagccggta tttctagcga aacagaagaa
    20701 cgaatggttc gtcgcacgtt gagccatctt gcgaacgcgg attatcgtgt gccctctccc
    20761 catattgccg aacggttgta tccgctcttt tatgaagagc ttggagattc ggatccctat
    20821 cggctgcgaa agcagcaaga caacgaatta gcttgtgcgc tagccaaaac actcgaaccg
    20881 aagtggaaga cctccagcca accgttgcgt gccttggtga acttggcgct cgctgccaac
    20941 gtcattgact acggagttgg taacgctttc gacttggagc ggaccatcga ccatgtaatg
    21001 agccaacaag cactggtcga ccatatcgat gcgttcgaag ctcggttggc ggattgtcga
    21061 aaaatattgt atctagctga taatgcagga gaaatcgctt tcgatcgcga gttgatcgtg
    21121 catggtcttg gtgcgcgacg cgttacgttg gtcgtacggg gtgggcccat catcaacgat
    21181 gtaacccgaa aggatgcgga ccaagtgggg ttgtcggcgc tggtgcccgt catcgatacg
    21241 ggcgttgccg taccgggcgt ggcatgggaa cggtcgagtc gggagttccg aaacgcattt
    21301 gaacaagcgg atctgattgt ctccaagggt cagggcaact tcgagaccct ggagcacttg
    21361 catgatccac gaatattttt cgtgttcatg atcaagtgtc gtgctgtgag ttgtctgagc
    21421 ggataccccg tgcatactgg cgtggcagct tttggtgatc gctttttcct aacaccgtcc
    21481 catcgctcat agcagcgtgt acaccacgct aactgcggtg atggcatcgg ttttttcgac
    21541 acccggcagc ggattatggt cgtacttgag ggtgaacgtg gttgcgatcg caaacgaatt
    21601 gccgatgctc gaggtaagcc cggcatccca cgtgaggcgc caattggtgg tttcctgtaa
    21661 gccttggatg tattcgagcc cggaattgaa cgttaccttc tcgttgatgg aattatcgta
    21721 tcccacaaaa gcacgggcgt tgtggcgtgt ctcggatttg tcgaccagct ttccttccaa
    21781 tgccgccgca tcgatggcgt cgtcgcgacg gatatcgtgc tgcaggtcgt agccgatttc
    21841 tgtccatagc tgatggcttt ttcggtcgat gaaataaaag gcgaaaccgg ggtcgatatt
    21901 gagacgcaag tccaagcctt ggaatcggtc gcgtgttcct gatgccgcca ggaatcccgc
    21961 gaggaccggc gtgaagaagc ggtcgtagcg tgcgcgccct tgcacgtttt caagggttgt
    22021 ttccatgcct tcgtcagcgg tgggcgatga cttggcatag ttgcccgctg ccgctgccgt
    22081 aaattggttg tctccccgac gaagcttgaa acgtcccgca ccggtgagcg acatggcacg
    22141 cgagtttccg gtggagaaca agccgcccgc ggaaatcttc aattcggtcg tgtctttggt
    22201 ctcttgatcc cgagccgttg ccgcttcgaa tccgctcgtg gccacatccg tttttccgtc
    22261 cgatgcagcc ttggtttcca tcaatccctt cggcaattcg tcgccgaacg ctggtgacgc
    22321 gatagccatc atcaaagcag tgccgaaagg tagtgaaacg cttttgctca tcatcctaag
    22381 aagtcctttc tttcgcgtta tgcgtaagag tcgttgggtt aagagttgcg gttccaacaa
    22441 tggattcccg ataaatccaa gaatatgagt ggttacaccg tttatgttga tggtgaccaa
    22501 gtgaacgatt ggtaagacag tgggcaatgt ccagcccttg actccaaaac tgtcataaac
    22561 caagcggtat cgacgcaaga tttcatgaag tggttcatag tgtgctatcc gtttggacgg
    22621 aatcattccc atgactgact ttgctttgcg cttcggacag cagaccattc ccatcgttgg
    22681 cacgatgctt atgggacgga gccctacttg ttcgcttgtt ttcaccgatg acagcaaagt
    22741 ttcgcgacag cacgcccggg tattcctcga gggcgggcag ttggtcattg aggatttggg
    22801 gagtaaaaac ggcgtatttc tcaacggggt tcggattgcg caccgtgcga gtatgcgcgt
    22861 gaacgatgtc ctcaatcttg gcaatcaatg cctcgagatt gtggagatga agcctgctcc
    22921 ctatcggaat acggctacga aacccgaagg ggttgttttc gatgaggacg acgaaccagc
    22981 gacggagacg ttgacaggag cggagcctgc cgattggcat gcgatcgagg cggcatggca
    23041 gcgagccgat atcgacctag cctcgatgct tatggatcag gcgtttgaag tcactgcgtt
    23101 gcgctcgatg gctcgtggtg agatcgacaa ggaagatcag gcttctatgt gtgacttggc
    23161 ccttcgaatg gccatcgaaa cgaaaactgg acgttggctc aatcgtctcg tgcaagttca
    23221 tattctcgta tccaaaccgt tgccgcgaaa tgtgacggct tcgttgccat cgctcgttca
    23281 taaggttcat ggtttggata agtcgctgtt tgagt
//
LOCUS       DAOSOR010000018        23195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_2226_length_23195_cov_4.735480, whole genome shotgun sequence.
ACCESSION   DAOSOR010000018 DAOSOR010000000
VERSION     DAOSOR010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 23195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23195
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2226_length_23195_cov_4.735480"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PK710_01805"
     CDS             <1..254
                     /locus_tag="PK710_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08468.1"
                     /translation="GLTGTLTYIVRLTNNATLAYPTYFVHLSLVAFLLSTIAYTKFAH
                     VFYRTTAMTYALHIGRTVEEICPALPAPPPQPALPEQAS"
     gene            369..881
                     /locus_tag="PK710_01810"
     CDS             369..881
                     /locus_tag="PK710_01810"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOT08469.1"
                     /translation="MDETTRNLMVGLREAMQAERYGQAFYQNAAMTTADPQGKEVFEQ
                     LAREEREHFEFLAAHYKSLLTHQTPAAGVRLVGHAKVDSASPIFSSQLHARIKEAHFE
                     MSALAIAVQLELNAITHYRAMAEKTSIPEVKRFFEELASWEKSHYDAFLHQQQALQDE
                     YWTASGFSPF"
     gene            983..1561
                     /locus_tag="PK710_01815"
     CDS             983..1561
                     /locus_tag="PK710_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08470.1"
                     /translation="MPQKKSERSPLGARTTHYGVAAIALGLSSYIWWVTGDPATPPVH
                     PKPTLYQDAVIDAPITLVTADKTDLSCAYEGDAEGYRCAFKKPNEAWPDVDPNNVEHR
                     KKMLVPYMTVDNQLFLIPGMFEEPAVDERYRDEVPRKLPRDKLERFTAQCKLLLRKEI
                     EGVMVRWAPDKDWQGPNKVWIGEVSNCQVSEP"
     gene            complement(1567..2115)
                     /locus_tag="PK710_01820"
     CDS             complement(1567..2115)
                     /locus_tag="PK710_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08471.1"
                     /translation="MLKARRGKEIDDSSSPQTVTHACLEAELRVADQVGRLMEFWGFK
                     RVHGRVWTILFLSAEPLSTSEIRKRLGISMGAASMALAELRRWGAVREAVTRHRAVHY
                     EPETNIWRLISRVMQDRERRILDESLEVFEQALSVLRTSAGPSAHRVERLVLLTKLVQ
                     GMLGMLLSRGELAAEDTKKMRV"
     gene            2295..4667
                     /locus_tag="PK710_01825"
     CDS             2295..4667
                     /locus_tag="PK710_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOT08472.1"
                     /translation="MPPSNAEGHSSAPGRPARRSGLLERIFPFIGWFRGYSSLALRMD
                     FIAGLTVALVLIPQSMAYAQLAGLPAYYGLYAAFLPPAIAALFGSSHQLATGPVAVVS
                     LMTAAALAPLATAGSTAFIAYAVLLSIMVGLFQLLLGVLRLGLVVNFLSHPVVNGFTN
                     AAALIIATSQLNKLFGVSVDSAEHHYETVWRVLVAATHYTHWPALALAILAIAIMVGM
                     RKINPKIPGVLVAVVLTTVLSWAIGYERNTKVPVERIQSNDIRETIAAFNHAIDAKED
                     LTKHKVEMLIPHLRETEQKDGKQSETYAAREHDIALVDLKIANLNLTIQELRARLRDT
                     QLSGVASTDDTHFYVKGAIPSGMQTSGPTWRLRVGNSKLNEAGILLIGGGAVVGEIPK
                     GLKFGLPTRDRDGNPFLPWAAIPGLLSSAVVISLLGFMEAISIAKAMAAKTGQRLDPN
                     QELIGQGLANIVGAFSQSYPVSGSFSRSAVNLQAGAPTGLSSVFSSLVVLATILLFTP
                     LLYHLPQSVLAAIIMMAVIGLINVKGFVDAWKAQRYDGVISVVSFLCTLAFAPHLDKG
                     IMVGVLLSLGLYLFRNMKPDIALLAKHPDGSFRSATRHNLSLCKHIAVLRFNATLFFV
                     NVNYLEEKVLELIATMPWLRHVLIVGDGINELDASGEEMLSMLVARLRESGFEVSLSG
                     LNDSVLDVLKRTRLYERIGEDHIFPNVSSAVEALHGPSHRGSDEKTCPLVYAHVDGLP
                     IAPTIKLNAALLHGPPESKAPHDFDRNDEAATNESTAPDDHATASRDGEA"
     gene            4670..5917
                     /locus_tag="PK710_01830"
     CDS             4670..5917
                     /locus_tag="PK710_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT08473.1"
                     /translation="MTFIGIFSGSYCHGDEVASRVARELGYDDVSRSLPEAAASRHKI
                     PLDRVLRSMEGPASLLDAFRRDRNRNVAYLREAIAQRVAGNSAIYHGFATHLLPNTVS
                     HVFKICLVAERSYRIEQAMRLDHVSEKKARKLVHAHDLQRLAWTQYLFDLGPWDERLY
                     DEIVPVSRMSVDEAVALIVSHARKPALARNAVSTGAAQDFHLASQVSIAVAERGHEVD
                     VTSADGIVTMVLNKYVVRVEHLEAELKRIASSVPGVKEARTRLGPKFRPVGIHGELAL
                     VGPNRLLLVDDEKEFVHTLSERLEARDLAAAIAYDGEDALSFVRGQTPDVIVLDLKMP
                     GIDGLGVLRRVKREHAGVEVIILTGHGSEVEQKTAESLGAFAYLQKPVDIELLASTVR
                     AAYRKVAEGRAELQATEPTDSEA"
     gene            5962..7683
                     /locus_tag="PK710_01835"
     CDS             5962..7683
                     /locus_tag="PK710_01835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT08474.1"
                     /translation="MELKLFVENNGGAKPDEKDDHLTGQDSYRRIWRTTVLITLMVAI
                     APLVIMTAINYFQYQKALTAEMSHPTSLILSRSKRSLEFFLAERISALRLVVKDGSLG
                     NLCDQDKLALGLRNINASFGGFIDLGSIDQDGFQCAYVGPYPLIGKSYKGQDWFQEVT
                     LRGEYVSDVFMGYRDAPHFVIAVRHDNPDGGFHVIRATVGAELLSHHATSLDLKPSSD
                     AFIINRDGILQTPSRFNGTFLELSQIPVPPYRAESQVIEQLDSRNKPYILGYAYVERS
                     PFILMMIKRPDDMMASWLTLRGDLIGFLIASVVLIVIVVLWSATSMINRIRHADLRRA
                     EFIHNIEYTSKMASIGRLAAGVAHEINNPLAIINEKAGLMKDLVSLSEDFPKKERFLK
                     NIQSITKSVERCSTITHRLLGFAKRMDVKLETIDLRELLQEVLSFLCKEAEYRNIHIE
                     FHVDEEIPSIESDRGQLQQVFLNIINNAVAAVLQGGHIDIWIEKTRDDRVRIRIQDDG
                     PGISKENLKKIFEPFFTTKKDYGTGLGLSITYGIVQKLGGTIDVQSEEGKGTSFIVTL
                     PLTVSNL"
     gene            7696..8082
                     /locus_tag="PK710_01840"
     CDS             7696..8082
                     /locus_tag="PK710_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT08475.1"
                     /translation="MDGMRVLLVDDEEELVETIAERLELRGIVAETVTNGEEALQRVL
                     EKPFDVVLLDVKMPGLGGMEVLKLIRMQRPNTQVILITGHASVENGDRRLLEGAFDFI
                     VKPVDIEVLVDKMKEATQRRKESDKP"
     gene            8079..8750
                     /locus_tag="PK710_01845"
     CDS             8079..8750
                     /locus_tag="PK710_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08476.1"
                     /translation="MNQPPSRSLESEGLAFFGIVTASISHELKNVAAIINESAGLLHD
                     LSVGAQAGRKPLDPARVMKLSADIVRNVERSVSILNRMNRFAHSVDEPKQTVDLFALC
                     RDTMALAARAAVSRGIELIDELPNQTAPIPSFPFGLYRLLYQAIQIAVLDVQGKPSVH
                     LSATLAPTLVRVQLRRDGSLMCTEEHRTRREAIEKLMQALGGCVRIDENMPDAMGVQL
                     ELPTS"
     gene            8795..9232
                     /locus_tag="PK710_01850"
     CDS             8795..9232
                     /locus_tag="PK710_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT08477.1"
                     /translation="MERDEKVLLVDDEVEFTSVLAERMQARGLTVDTAASGVEALEKM
                     EGGSRYDVVVLDLAVPGMNGMQTLERLMALNPDMQVILLTGHATLQDGIRAVRMGARE
                     VMEKPADLSELLQKISEAKLDKIMLIEKRMEEQIQEILNAKGW"
     gene            complement(9314..11218)
                     /locus_tag="PK710_01855"
     CDS             complement(9314..11218)
                     /locus_tag="PK710_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08478.1"
                     /translation="MRRWPNWGTWLLYLAGGFGVACATNEGDTRAYAPPGSKKTDSGG
                     AGESGAGGSGGHGGVGDAQWPDGAGASAGAGASAGAGASAGAGAMGGAAGSATGGTGA
                     VGGAAGSAGAGGIGPCGAEACSIAGTCVEAGTPNPSDPCQACLPVLHGSAWSKDDANV
                     TCHDSPYWTGVKRELPRTPYQHENAVSCHNCYAGTLSGTLNKIHAAQAAGADLIELDI
                     KDQDGTVYVEHNDTGGTSAPTLVEVLADAALKSGSQLLFIETKETTPTESYLRAVFDH
                     LMAHGYGVAGRPVVLRTFDALRNNLLIARKLLATPAYASLRKHVRLHVLFSRNEGSNI
                     AALQGKVRKCKNDGFHGVEFEYQTPNLFGALTYAKSLRLGTSVWTIPTSMGEAFISAL
                     RDEVDAVTVDYSVSVARAVIQDNNALIYLNVWDQPATASSMRWYGVSDASLHQAPVNV
                     AGAPTTESLSMGEDRFGTSLIFKAADSQSVTFFDADQTANAGYFVAVVVNFDRLTLPD
                     GETQEVIGKANSGGFALELFNPPGAEDTVLRFGVFVEGEYRYATYPASKLDGSQSYFI
                     IGAYDGDGAVRMWINNDATGTTASGSFKAGVVQNDVPVLLGADPQVGPSRFFFDGKVQ
                     MALVQNWKNH"
     gene            11435..12049
                     /locus_tag="PK710_01860"
     CDS             11435..12049
                     /locus_tag="PK710_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HOT08479.1"
                     /translation="MMKLYYMPGACSLASRIVLEWTGASWVNEKVGHEQLHQEAYLRI
                     NPMGAVPALQVDDTIITQNSGILTYLAEAFPKARLGGGETPLGKAEVHRWLGFINSDI
                     HPTFKPFFGSTSFLGDDTMIEKTRTEARRLLRNMFEIIDRQLKGRDWIAGERSIADAY
                     LFVVLGWTKGVGVDLSGFDQITRFVERMNQDPGVQRALQFEKQA"
     gene            complement(12062..13447)
                     /locus_tag="PK710_01865"
     CDS             complement(12062..13447)
                     /locus_tag="PK710_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT08480.1"
                     /translation="MGRRPTEFADTGTHLIARPKLASSVSFLLLVAEGPDKGRKFVLD
                     SSSPSRSLTGKSASCQICLTDPSVSRRHAAFDVVDRRLRLTDLGSSNGTFVNGISIVE
                     VYLDGGEFVRMGQTVFHVQQEEKGKEVALSQRRTFGRVVGGSEEMRRLYPLCERLAGS
                     DVPVLIEGETGCGKEVLAESLHEMGPRANGPFIVFDCMSAPPTLLESALFGHEKGAFT
                     GATSSRAGVFEQAHRGTLLIDEIGDLDISLQPKLLRAVQRMEVQRVGGSKPIKVDVRV
                     LSATRRNLDEEVAAGRFRDDLFFRLNVARIELPPLRQRRGDVALLARHFWDMLGGKER
                     PIPQSLMERLEAYSWPGNVRELYNTIARHLALGELAGEMIGDGARLTDGPASVGDSNE
                     LRGHEDYIDEVVAQRLPLPEARQQVMDEFERRYIDAALMAHGGNVTKAAAASGVGRRY
                     FHLLRARVTKT"
     gene            complement(13532..14791)
                     /locus_tag="PK710_01870"
     CDS             complement(13532..14791)
                     /locus_tag="PK710_01870"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HOT08481.1"
                     /translation="MLAQELFVSLIQVGGWIHSRALVHGHRGDAGVDERCVDPAALAV
                     SNGRMKASGIVLGVAALGMVGCASPAQKCANDRDKNLESCEIACENGDLPSCVSAARA
                     YDQRYMKSGSVSDAERSVSMYEKSCLAGRKEACFGAVRGILLGPSESNPELSPPARVD
                     RAAERRRVLLAKGCELDDPQFCKEASDAFLGEDAQRSEQLGRKYCVLELTDKGKRDAC
                     VMQREQRARQAQQGANRCASGQPGGCLALGNSVVTVDRVRAREAYARECKGRRLDVQG
                     NADACVKVYESQGLATKLPAVEPSISDCRDARVILRSLRLIPESPSRPNPEQVREVVD
                     RGTEALERCYAAGLRKNPKLAGSLTIRATIDGLGEIWNVQEDDIALIDLPTVECVKKE
                     ATTWRFPEPSQGTIQLEAKLAFRVERP"
     gene            complement(14761..16341)
                     /locus_tag="PK710_01875"
     CDS             complement(14761..16341)
                     /locus_tag="PK710_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08482.1"
                     /translation="MLDRRFALLIPVGLVLACGSTVQEEGQGSEAGQATVVPPGKADN
                     YLSPTSMEYRLWGEGQLDLDESWLDKTDDQKRAQAEQLLAYKFKAYTHFINVYVTDKS
                     SHDANHGYGGFSGLVRKSSLDSMVEPIDDLGLSYTFLWELEMGGPRALLSNLPIETNS
                     AGEKTFRVKLPVLTESQLKYSSYPKDFNASTYSGELEEIQVAIEPEKESFDAFPEYNR
                     LFEDGKLDILVLVGGDYNEERYDLRAADEIFQWLKRAGYAHPASRYTDLALDSPPFTS
                     VLDVQGRKIDVEITLLHPSIVSVGDLDKLRAKIIQAYQTMDVLIYDGHAGEDPSYSGL
                     VYHYNPRHAISADELSKISLPEKYQVYVFNGCKTYGVYPESIMKNPAKTTANVDIIST
                     VNFSWLTMQPYTTSGFINQLLAKSAGTHDPQTYLQILRQINQSSNWNVYYGVHGLDDN
                     PHVNPYADISSLCRSCATDADCPGVGNRCIAFSSGKACGVECTAPDGCPSGYGCFDIA
                     EGNQIVGQQCLPKSYSCR"
     gene            complement(16469..17095)
                     /locus_tag="PK710_01880"
     CDS             complement(16469..17095)
                     /locus_tag="PK710_01880"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT08483.1"
                     /translation="MQLKEAMEARTSVRTYLPEPVSVDDLKEMVRLAGRAPSLNNAQP
                     WKYYAVVEASRCQRIASLVEQKLHTILPEPKTDAQQKATSKLFDYVGLFGRAPALLIV
                     ATRIYPGFLDDALMTTGLAREAADAVRGHPYAMSLGASIENLLLAATDMGYGACWLTV
                     PQVASASIERDVGIEAPWRLGAVISVGRPAQEAIQSPKKPIEEIFSVL"
     gene            17250..17615
                     /locus_tag="PK710_01885"
     CDS             17250..17615
                     /locus_tag="PK710_01885"
                     /inference="COORDINATES: protein motif:HMM:NF020400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HOT08484.1"
                     /translation="MNIAIMGCKEIKDETCIGCQRCLTAFHRREGEFSRYKDQPDARI
                     VAIFHCGGCPATSPVLRMKQLQTWMSPMGERVDVLHLGTCVKNHCAYKDTLVSAVRNK
                     AGVEVIEGSHPYLPKDIFA"
     gene            17915..18958
                     /locus_tag="PK710_01890"
     CDS             17915..18958
                     /locus_tag="PK710_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08485.1"
                     /translation="MKRTTRSLVFALSVAALLVGGFLLFHRSQRQRVAHQPLAAVPAD
                     AQFILTANMARLRSSPYRNLLRDASMSPLTAGAQESCGKNLEERIETLVVWKPAEPGS
                     TMGIAAYAPVSAQSVWECAKGTMDSRGAQATFSHVEGFQIITDETLGPGSAQIAVRDP
                     GLLVVARPGTRARMLDAFAGRCPSAFDQGTHARLRKELPKGDVVLTLTVNPAFRQRVA
                     DWVDEPSALDHVGEIGLSAILQPTTELQVVVVCESDHVCQSLEQRLQAIRTRARRSML
                     LRSMGVAGWLDATTLRQGNQRLHLHASLSAEQVLDLWQRLSTDPQEPPPSFVPALRSS
                     SDETLRPPSSSPP"
     gene            19048..19332
                     /locus_tag="PK710_01895"
     CDS             19048..19332
                     /locus_tag="PK710_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08486.1"
                     /translation="MTGVLQHVGDDAPPRCVHCGKQAAGPCASCHRMICADCCTLTES
                     SVKKWAICLECDRKKGRSLGHAWGSLILWLLLPLGILAVATALVGLLAHL"
     gene            19891..21492
                     /locus_tag="PK710_01900"
     CDS             19891..21492
                     /locus_tag="PK710_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08487.1"
                     /translation="MRFFPFSYYRFMVAGTLLFALSDCSGDEFTQEAADASVDSGGPQ
                     EASTGGSGGTSTGGAGGATGGSGGAPTGGAGGATGGTGASAGHAGTGGDAGPEPTCDP
                     NESPPTDAIYVSAKEGDDNASQGSPEQPLQSLSNAIKLAVHNQIGKIILDQGTYKEAV
                     VLSLQDPLVIMGGWIKVGTAWTRDCSQTARNKTLLHSPTSTGLQITGNSSVSLQTMVI
                     QSTGTNNAAPDNQSGESCYGVFVSGAELSMNDVTIRACGGQDGGKANKYEEVQPPDCS
                     QLQGTGCSMGEHGGSGSGGQDAKEAGTFTLNGYTPANGQHGNYGSNGNNGTPGNPGNT
                     AQCYEKGCTGQQVSGSCPSNHYCGNETPQKDTSSQPGSCGCGGRGGSGGAGGRGGGGS
                     FGIYATGNASVLVKNSRVEAASGGNGSPGQTGQIGLNGLDGKQGDSVMCYTQCKPMQV
                     GEKCNCEQPGSYQLAGGEKGGKGGNGGNGGPGGAGSGGPSIAALIHGSATISHLNVDF
                     LHSTAGTPGSPSAAQGDAKDILEVQ"
     gene            21648..21956
                     /locus_tag="PK710_01905"
     CDS             21648..21956
                     /locus_tag="PK710_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08488.1"
                     /translation="MLSTSHRMRWLVDLHGRRHVVDASWPGFSNWIAVDGHTVERWRW
                     PSNNLWTWRTFPLAGSSARIIRRRSGLFSFAYELQVDAPGAHVQQVDPWSSLWWNGRV
                     "
     gene            complement(21993..>23195)
                     /locus_tag="PK710_01910"
     CDS             complement(21993..>23195)
                     /locus_tag="PK710_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08489.1"
                     /translation="VEQAIPAVGREQVDALADRVGYHLLEAGEKHRAARLFALSAGYR
                     KRSGQLEGALSDYLRAFDLVSLADCPVDTLLQWLAELRDVVRRVRAGASLLDVLQRVV
                     ARVDKEATLSQRVMARVDGASMLTYAHRFDAAMVLLDEALELAGSNEPIRSLTLLTQA
                     QLFGIRGEFGAAVTIYEQMGDRLFEGKDAVRYDMGLAQAYGATGQKKRALEYLERATA
                     ISEPGDVGAAMDREKLRALIFHFSGDFKEAAMASARGADLAQSIGLSYETALGLHNLG
                     DSWLWVEDFARSHTAFQRSLAIAEQYGYDRLARHDRMYLWYLEGRQGAEHAIESLRLL
                     VRKAEDEGLTWDALDGRFLLGALAYRLGQSDLAREELERTRILARECRSRLIEKTAVE
                     LLERIGSQ"
BASE COUNT         5257 a         6921 c         6175 g         4842 t
ORIGIN      
        1 cgggactcac cggtacgttg acgtacatcg ttcgtctcac gaataacgca acgctcgcct
       61 accccaccta cttcgttcat ctatcgctcg tggcttttct gctcagcacg atcgcctaca
      121 cgaagttcgc gcacgtattt taccgcacga cggccatgac ttacgcgctt cacattggcc
      181 gtacggtcga agagatatgt cctgccttac cggcaccgcc cccacaaccg gcgttgcccg
      241 aacaagcctc ctgattggcg actcctccgc ctgacgagca tccgattgtc tgcgacgctt
      301 tcgtcaggtc gtagtaaacc agccatggct tgcaccgcgt gcggcattgg aatcgaaagg
      361 ataagctcat ggacgaaacg acacgaaacc tgatggtagg gctgcgtgag gcgatgcaag
      421 ccgagcgtta tggacaagcg ttttatcaga atgcggccat gaccaccgca gaccctcagg
      481 gcaaagaagt gtttgagcag cttgcgagag aggaacgcga gcatttcgag tttctcgcgg
      541 cacattataa gtccttgctg acccatcaga cgcccgcggc gggcgtacgc cttgtgggcc
      601 atgcgaaggt ggactcggcg agcccgatct tttcgagcca acttcacgca aggatcaagg
      661 aagcgcactt cgagatgagt gcgttggcaa ttgcggtgca gctcgaactc aatgcgatca
      721 cccattatcg agcgatggcc gagaaaactt cgatccctga agtcaaacgc ttcttcgaag
      781 aactcgcgag ttgggaaaaa tcccattacg acgccttcct ccatcagcag caagctttgc
      841 aagatgaata ctggacggcg tctggatttt ccccgttctg agaccatcga ttcgccgaca
      901 acggccacga tttcgggttc ttgttcggaa ccgtattctc cccactgcgc aattggcttc
      961 ttgctgctag gctacgcgtc ccatgccgca gaaaaagtcg gaacgttcgc ccttgggagc
     1021 caggacaacc cattatgggg ttgcggcgat tgcccttggt ttgagttcgt atatttggtg
     1081 ggtgacggga gatccagcga cgcctccggt acatccgaaa cctacgttgt accaagacgc
     1141 ggtcattgat gcgccgatca cgttggtgac cgcggacaag acggacttgt cctgcgcgta
     1201 tgagggagat gcggaggggt atcgttgtgc tttcaaaaag cccaacgaag cctggcccga
     1261 cgtggatccc aacaacgtgg agcatcgaaa aaagatgctc gtgccctata tgacggtgga
     1321 caatcagcta tttttgattc cgggtatgtt cgaggaaccg gcggtggatg agcgttatcg
     1381 cgacgaagtt cctcgcaagc ttccccggga caagctggaa aggttcacag cgcagtgcaa
     1441 gctattgctt cgtaaggaga ttgagggggt gatggtgcgt tgggccccgg acaaggactg
     1501 gcaggggccg aacaaggtat ggattggcga ggtgagcaac tgccaagtca gcgagccttg
     1561 atggggtcac acccgcattt tcttcgtgtc ttccgcggcc aattctccac gactcaaaag
     1621 cattccgagc attccctgca cgagtttcgt caacagcacg agtcgttcca cgcgatgggc
     1681 ggaggggcct gcgctcgttc gaaggacgga caacgcttgt tcgaatactt cgagggattc
     1741 gtcgagaata cgtcgctcgc gatcttgcat cacccgggag atgaggcgcc aaatattcgt
     1801 ttccggttca taatgaacgg cgcgatggcg cgtcaccgcc tcacgcaccg caccccagcg
     1861 tcgaagttca gccagcgcca tactcgccgc acccatggaa atcccgagac gtttgcggat
     1921 ctccgaggtg gacaagggct ccgccgacaa aaacagaatt gtccaaacgc gaccatgcac
     1981 acgtttgaaa ccccagaatt ccatgagacg gcccacttga tccgccacgc gaagttccgc
     2041 ttccaaacac gcgtgggtga cggtttgcgg cgatgagctg tcgtcaatct ccttgcctcg
     2101 acgcgctttg agcattggtc accacaatag aagcgttgag cacaaattgc acccaccgga
     2161 tgcgaagcaa aatgttgggc aggagcatca tgcccatgaa aagagccgcg tcagaaacgc
     2221 cttcgtgccg tgaaaaattc gtcttcttca cgcattgtct tggttgattc ggccgggtgc
     2281 ggtaaacgtg aatcatgcca ccgtcgaacg ccgaggggca ctcgtcggcg cctggacgcc
     2341 ccgctcggag atcgggtctt ctcgagcgca tctttccgtt cattggctgg ttccgagggt
     2401 actcgagcct cgcccttcgc atggacttca tcgcgggact caccgtcgcg ttggtgttga
     2461 tcccacagtc catggcctac gcgcagctcg cggggcttcc tgcctactac ggcctatacg
     2521 ccgccttttt gccaccggcc atagccgcat tatttggatc gagtcaccag ctcgccacag
     2581 gtcccgtggc ggtggtatcg ctgatgaccg cagccgccct cgcgcccctc gccaccgcgg
     2641 gaagcactgc gttcattgcg tacgcggtgc ttctttcgat catggtcggc ttgtttcaac
     2701 tgcttttagg cgtgttgcga ctcggacttg ttgtcaactt cctatcgcat cctgtcgtca
     2761 acggattcac caacgctgcc gcgctcatca tcgccacctc ccaactcaac aaattatttg
     2821 gtgtttccgt cgatagcgcc gaacaccatt acgaaaccgt ctggcgcgtc ttggtcgccg
     2881 caacgcatta cacccattgg cctgcgctcg cccttgccat cctggccatt gccatcatgg
     2941 tcggaatgcg aaaaatcaac ccaaagatcc ccggcgtgct cgtcgctgtc gtcctaacca
     3001 cggttctttc ctgggcgatc ggatacgaac gcaatacgaa agtgcccgtc gaacggattc
     3061 aatccaacga tattcgtgaa acgattgctg ctttcaacca cgccatcgac gcgaaagagg
     3121 atctcaccaa gcacaaggtg gagatgctca tcccgcatct tcgagaaacc gaacagaagg
     3181 atggaaagca atccgagacg tatgcagcac gggagcacga cattgcgctc gtagacctca
     3241 agatcgcaaa tctcaacttg accatccaag agcttcgagc acgtcttcga gacactcaac
     3301 tatccggcgt cgcatccacc gacgataccc atttctacgt caagggcgcg atcccttccg
     3361 gcatgcaaac cagcggccct acgtggcgtc ttcgcgtcgg aaacagcaaa ctcaacgaag
     3421 ctggcattct attgataggc ggaggcgcgg tcgtcggcga gattcccaaa ggattgaaat
     3481 tcgggcttcc cacccgtgac cgggacggca acccatttct tccttgggcg gccattcccg
     3541 gactgctgtc gtccgcggtg gtgatctccc ttctcggatt catggaagcg atctcgattg
     3601 ccaaagcgat ggcggcaaag acgggacaac gtctcgatcc gaaccaagaa ctcattggtc
     3661 aaggcctggc gaatatcgtg ggagcttttt cccaaagcta ccccgtctct ggttcgtttt
     3721 ctcgatctgc agtcaacctt caagcgggag cccctacggg tctatccagc gtattcagca
     3781 gtttggtggt attggccacg atcttattgt tcacaccctt gctttaccat ctgccccaat
     3841 ccgttcttgc ggcgatcatc atgatggcgg tgatcggcct catcaacgtg aagggctttg
     3901 tcgatgcctg gaaagcgcag cgatacgacg gcgtcatttc ggtggttagc tttttgtgta
     3961 cgcttgcgtt tgcgcctcac ctggacaagg gcatcatggt gggggtgttg ttatccctcg
     4021 ggctttatct atttcgcaat atgaagcctg atattgctct tctcgccaaa catcctgacg
     4081 ggtcttttcg cagtgccaca cgacacaatc tttcattgtg caaacacatc gcggtcttac
     4141 gtttcaacgc cacgttgttt ttcgtgaacg tcaactatct cgaggaaaag gtgctcgagc
     4201 ttatcgcgac catgccatgg ttgcggcacg tccttatcgt aggcgacggc atcaatgagt
     4261 tggatgcctc tggggaggag atgttatcga tgctggtggc gcgccttcgg gagtccggtt
     4321 tcgaagtatc gttgagtggc ttgaacgact ctgttctcga tgtgttgaaa cgaacgcgtc
     4381 tttacgaacg aatcggggag gaccacattt tcccgaacgt atcttccgcg gtggaagcac
     4441 tccatggacc gagccatcgt gggtccgatg aaaagacttg tcctctggtg tacgcgcatg
     4501 tcgatggtct tccgattgca ccgaccatca aattgaacgc ggccctcttg catggcccgc
     4561 cggaatccaa ggcgcctcat gatttcgatc gtaacgacga ggccgccacg aacgagtcga
     4621 cggcacccga tgatcatgca acggccagcc gcgatgggga ggcgtgaaca tgacattcat
     4681 tgggatattc agcggctctt actgtcatgg cgacgaggtg gcatcgcgtg tggcgcgcga
     4741 gcttggttac gatgacgtga gccgttccct tccggaagca gcggcgtcgc gtcataaaat
     4801 tcccctcgat cgcgtcttgc gatccatgga gggtcccgca tcgttactcg atgcatttcg
     4861 gcgcgatcga aaccgcaacg ttgcgtatct tcgcgaagcc atcgcgcagc gggttgccgg
     4921 aaatagcgct atctatcacg gcttcgccac acatcttctg cccaacaccg tctcgcacgt
     4981 cttcaagata tgtcttgtgg cagaacgttc gtatcgtatc gagcaagcga tgcgcttgga
     5041 tcatgtttct gagaaaaagg ctcgcaagct cgtccatgcg cacgatctgc agcgtcttgc
     5101 gtggacacaa taccttttcg atcttggacc gtgggatgag cgtttgtatg acgagatcgt
     5161 tcctgtcagc cgcatgtccg tcgacgaggc ggtcgcgctg atcgtatcgc atgcgcgaaa
     5221 gccggccttg gcgcgcaacg ccgtttccac tggggcggca caggattttc accttgcctc
     5281 gcaggtaagc atcgcggtgg cggagcgcgg ccatgaagtg gacgttacat cggcggacgg
     5341 tatcgtcacc atggtcctca acaaatacgt ggtgcgcgtg gaacacctcg aggcggaact
     5401 caagcgcatt gcatcatcgg tcccaggcgt aaaagaagcc agaacccgcc tgggacccaa
     5461 gttcagaccc gtgggaatcc acggcgagct ggcactggtg gggcccaatc gactcttgct
     5521 ggtcgacgac gaaaaagaat tcgttcatac gttgtccgag cgtcttgaag ccagagacct
     5581 tgcggcagcg attgcgtatg acggggaaga tgcgttgtct ttcgttcggg gccagacgcc
     5641 cgacgtgatc gtgttggatt tgaagatgcc ggggatcgat gggctggggg tattgcgacg
     5701 tgtcaagcgc gagcatgcgg gggtcgaggt gatcattttg acgggacacg gctcggaggt
     5761 tgagcagaaa accgccgaga gtctcggggc cttcgcctat ctgcaaaaac cggtcgatat
     5821 cgaattgctg gcgagcacgg tgcgcgcggc ctatcgtaag gtggcggagg gtagggcaga
     5881 gcttcaagcc acggagccca cggatagcga agcctagcca gtttgcttgg ggacggtcga
     5941 aggggaggcc gaaacgcaag gatggaactc aaattattcg tggagaacaa cggtggagcc
     6001 aagcccgacg agaaagacga tcaccttacg ggccaggatt cctatcgtcg catctggcgc
     6061 acgaccgttt tgatcacgtt gatggtggca attgccccgc tcgtgatcat gacggccatc
     6121 aattactttc aataccagaa agcgctgacg gcagagatga gccaccccac gtcgctcatc
     6181 ttgtcgcgaa gcaagcgttc tttggaattt tttctcgcgg agcgcatttc cgccttgagg
     6241 ttggtggtca aagacggctc gcttggcaac ttatgtgatc aagacaagct ggcccttggc
     6301 cttcgaaaca taaacgcgtc gtttggcggc ttcatagacc tgggttccat cgaccaggat
     6361 ggttttcaat gcgcctacgt cggaccttat ccgctcatcg gaaagagcta caaaggccaa
     6421 gactggtttc aagaggtgac gttgcgcggt gaatacgtca gcgacgtgtt catggggtac
     6481 cgtgatgcgc ctcacttcgt catcgctgtt cgtcatgaca atcccgatgg cgggtttcat
     6541 gtcattcgcg ccacggtggg agcggaattg ttgagtcatc acgcaacgtc tttggatctc
     6601 aaaccttcga gtgatgcgtt catcatcaat cgggatggca ttttgcaaac accgtcgaga
     6661 ttcaatggaa cattcctgga gctatcgcaa atcccagtac cgccataccg cgcggaatcc
     6721 caagtcatcg agcaactcga ttcgcgcaac aagccctata tccttggcta cgcctatgtg
     6781 gagcgatcgc cgttcatcct catgatgatc aagcgtcccg atgacatgat ggcaagttgg
     6841 cttactcttc gaggagactt gatcgggttt ttgattgcga gtgtggtgct catcgtcatc
     6901 gtggtgttgt ggagcgcaac gtcgatgatc aatcggattc gtcacgcaga ccttcgacgc
     6961 gcggagttca ttcacaatat cgagtacacg agcaagatgg catcgattgg ccgtctcgca
     7021 gcaggagtgg ctcacgagat caacaatccg ctggcgatca tcaatgaaaa agctgggctg
     7081 atgaaggatc tcgtttcttt gagcgaggac tttccgaaaa aggaacggtt cctcaaaaac
     7141 attcaatcca tcaccaagtc cgtagagcgc tgcagcacca tcacgcatcg gctgctggga
     7201 tttgccaaac gaatggatgt gaagctcgaa accatcgatt tgcgggagct attgcaggag
     7261 gtattgagct ttctgtgcaa ggaagccgag tatcgaaaca tccacatcga atttcacgtg
     7321 gatgaagaga tcccgtccat cgaaagcgac cgcggacaac ttcaacaagt gttcttgaac
     7381 atcatcaaca atgcggtggc cgcggtgctg caaggaggcc acatcgacat atggatcgag
     7441 aaaacccggg atgatcgggt tcgaattcgt attcaggacg atgggcctgg aatctcgaaa
     7501 gagaacctga agaagatctt cgagccgttc ttcaccacga aaaaggacta tggcacgggc
     7561 ctgggtctct cgatcaccta tggtatcgtg cagaaactcg gcggaaccat cgatgtgcaa
     7621 agcgaggaag gaaagggaac atctttcatc gttaccttgc cgttgacggt gagcaatctg
     7681 tagggagggc aagatatgga cggaatgagg gtattgctgg tcgacgacga agaggaattg
     7741 gtggaaacga ttgccgagcg gctcgagctt cgtggcatcg tggcggaaac ggttaccaat
     7801 ggcgaagagg ctcttcagcg cgtgctcgaa aagccgttcg atgtggttct gctcgacgtg
     7861 aagatgccag gtcttggtgg tatggaggtc ctcaagctga ttcgaatgca gcgtccgaac
     7921 acgcaggtga tcctcatcac cggacatgct tcggtggaaa acggggaccg gagactgctt
     7981 gagggtgcat tcgatttcat cgtcaaaccc gtcgatatcg aggtgctcgt cgacaagatg
     8041 aaggaagcca cccaacgacg aaaagagtcc gacaaaccat gaaccaacct ccaagccgat
     8101 cgctcgaatc agaaggactt gcgttcttcg gaatcgtcac cgcgagcatt tctcacgaac
     8161 tgaaaaacgt ggcggccatc atcaatgagt ccgccggatt gctccacgac ctatccgtcg
     8221 gggctcaagc tggcagaaaa cccctagacc ccgcgcgagt gatgaaacta tccgcagata
     8281 tcgtccgcaa tgtggaacga tccgtcagca tcctcaaccg catgaaccgc ttcgcccatt
     8341 ccgtcgatga accgaagcaa accgtcgatc tttttgccct gtgccgagac actatggccc
     8401 tcgcggcacg cgcggccgtg tctcgaggca tagaattgat cgacgaactt cccaatcaaa
     8461 ccgcacccat tccgtctttc cccttcggac tgtatcgtct cctgtaccag gccatccaaa
     8521 tcgccgttct cgatgtgcaa gggaaaccgt cggtccatct ttcggccact ctggcgccaa
     8581 cgcttgtccg ggtgcagctt cgtcgcgacg gcagcttgat gtgtaccgag gagcatcgaa
     8641 ccaggcgtga agctatcgag aagctcatgc aagcgcttgg cggatgtgtc aggatcgatg
     8701 agaatatgcc cgacgcgatg ggcgtgcagc tcgagttgcc cacgtcctga tcgagccgga
     8761 tttatcgcga gtgtggatca aggaaggggt cgagatggaa cgcgacgaga aagtattgtt
     8821 ggtggacgat gaagtggagt tcacgagcgt gctggcggaa aggatgcagg cgcggggact
     8881 tacggtggac accgccgcga gcggtgtaga ggcgctcgaa aaaatggaag gagggtcgcg
     8941 ctatgatgtc gtggtgctcg atctggcggt gcccggcatg aatggaatgc aaactctcga
     9001 aagactcatg gcgttgaatc ccgacatgca agtgattctt ttgacaggac acgccacgct
     9061 tcaagacggg attcgcgccg tgcgcatggg agcccgagaa gtgatggaaa aaccagcgga
     9121 tttatccgaa ttgctccaaa aaatctccga agccaaactc gacaagatca tgctcatcga
     9181 aaagcgcatg gaagagcaga ttcaggaaat cctcaacgcc aaaggttggt agatagcctc
     9241 ccaaccacta accatctcga gccaatcgtc atccaagccg ttgcgcgagt tcgaggccat
     9301 cgtgcaggtt cgatcagtgg tttttccaat tctgcacgag cgccatctgc accttgccat
     9361 caaagaaaaa ccgggaagga cccacctgcg gatcggcgcc caacaacacg ggaacatcgt
     9421 tttgtaccac gccggccttg aaggaaccgg atgcggtcgt ccccgtggca tcgttgttga
     9481 tccacatccg aacggctcca tccccatcat aagctccaat gatgaagtac gattggctgc
     9541 catcgagctt cgacgcggga taggtggcgt accgatactc gccttcgaca aacacgccga
     9601 agcgaagaac ggtatcctcc gcgccggggg gattgaatag ctcgagggca aaccccccag
     9661 agtttgcttt gccaatcacc tcttgcgttt caccatcggg cagtgtgagt cgatcgaagt
     9721 tcacgaccac ggccacaaaa tacccagcgt ttgccgtctg atccgcatcg aaaaaagtaa
     9781 ccgactgact gtccgctgct ttgaagatga ggctggtgcc aaaacggtcc tctcccatgg
     9841 acaacgactc ggtcgtaggg gcacccgcga cgttgacggg cgcctgatga agcgatgcat
     9901 ccgaaacgcc ataccaacgc atggaactgg cggtagcggg ttgatcccaa acgttcaggt
     9961 agatcagagc gttgttgtct tgaatcaccg cccgcgccac cgatacggaa taatctacgg
    10021 taaccgcatc gacctcatcg cgcaacgcgg agatgaaagc ctcacccatc gatgtcggga
    10081 tggtccatac cgaggttccc aaacgaaggc ttttggcata ggtcagcgcg ccaaatagat
    10141 tgggcgtttg atactcgaat tcgacaccgt gaaagccgtc gtttttgcat tttctgactt
    10201 tcccttgcaa cgcggcgata ttcgacccct cgtttcgaga aaaaagcaca tgaaggcgca
    10261 cgtgcttgcg caaagacgca tacgctggcg tggcaagcag ttttcttgcg atgagaaggt
    10321 tgttgcgaag cgcatcaaac gtacgaagaa cgacaggtcg ccccgccaca ccatagccat
    10381 gggccatgag gtgatcgaat acggctcgca gatagctttc cgtaggcgtc gtttctttgg
    10441 tttcgatgaa caatagctgg ctacccgact tgagcgccgc gtccgcgagc acttcgacaa
    10501 gggtgggcgc cgaggtcccg cccgtgtcgt tatgctctac atacacggtt ccgtcttggt
    10561 ctttgatatc gagttcgatc agatccgccc ccgcggcttg cgcggcatga attttgttga
    10621 gcgtgccgga aagggtcccg gcatagcagt tgtgacaaga tacggcgttt tcatgctgat
    10681 acggcgtacg aggcaactcg cgcttcactc ccgtccaata aggagaatca tggcatgtca
    10741 cattggcatc gtccttcgac caggcggaac catgaaggac tggcaaacag gcttgacaag
    10801 gatccgaggg attgggcgtt cccgcctcga cacaggttcc cgcaatggaa caggcttccg
    10861 caccgcaagg accaatacca ccggcacccg ctgaacccgc cgcaccaccc actgcccctg
    10921 tgccacccgt tgccgaaccc gccgctccac ccatcgcacc ggctccagca ctcgcaccgg
    10981 ctccagcact cgcacccgct ccagcactcg caccggcccc gtccggccac tgcgcatcgc
    11041 ccacaccgcc atggccccca ctgccgcccg ccccggattc gcccgctcca cccgaatccg
    11101 ttttcttcga accgggcggg gcataagcac gcgtgtctcc ctcattcgtg gcacacgcca
    11161 ctccaaaccc accggccaag tataacaacc aggttcccca atttggccat cgtctcatgg
    11221 catcaagagt accacttcga gcatcgactt gcagaaacac aactatccgc gccgcgcacc
    11281 cccctgcccc ttgcctcatc tccccatcgg ctccagagca gccaagtcgc gttcaacatc
    11341 gtcaatccaa tcgcagtcga acgcctcgga ttcatcacaa ccagttgaaa taactactat
    11401 ttttaccatt tcggcaccaa ggagatccaa gacaatgatg aaactctact acatgcctgg
    11461 agcctgctcg ctcgccagtc gaatcgttct ggaatggaca ggggcgagtt gggtaaacga
    11521 aaaggtgggg cacgaacaac tgcaccaaga ggcttatctt cgaatcaatc cgatgggcgc
    11581 ggtgccggcg ttacaggtcg acgacacgat tatcacgcaa aattcgggca tcttgaccta
    11641 cttggcggaa gcgtttccga aagctcggct cggaggcggc gagacaccgc tgggaaaggc
    11701 cgaagtgcat cggtggttgg gattcatcaa ttccgacatc caccccacct tcaaaccatt
    11761 ttttggttcc acatcgtttc ttggcgacga caccatgatc gaaaagactc gaacggaagc
    11821 caggaggcta ttgcgaaata tgttcgagat catcgatcgg caattgaagg ggcgcgattg
    11881 gatcgcaggg gaacgctcga tcgccgatgc ttatctattc gtggtgcttg gctggacgaa
    11941 gggagtagga gtcgatctct cgggctttga ccaaatcacg cgattcgtgg aacgaatgaa
    12001 tcaagatccg ggagtgcagc gggcgcttca attcgaaaaa caggcctgaa cacgacgtgc
    12061 atcaggtttt cgttaccctt gctcgcagca gatggaagta tcgacgcccc acgccggagg
    12121 cagcggcggc tttggtgaca tttcccccat gagccatcaa agccgcatcg atatatcgtc
    12181 gctcgaactc atccatgact tgttgacgcg cttctggcag aggcaaacgt tgtgccacga
    12241 cttcatcaat gtagtcttca tggcctcgca gctcgttcga atcgccaaca ctcgcgggac
    12301 catcggtgag ccgtgcccca tcgccaatca tctcccccgc caactcgcca agggccagat
    12361 gtcgggcgat cgtgttgtat agctctcgca catttccagg ccaggaatag gcttccagcc
    12421 gctccatcaa cgattgcgga atcggtcgct ctttgcctcc caacatatcc cagaaatgac
    12481 gagccagcag agccacatcc cctcttcgtt gacgtaaggg aggaagttcg atccgtgcga
    12541 cgttcaaacg aaaaaacaag tcatcgcgaa atcttccggc cgcgacttct tcatccaggt
    12601 ttcgacgggt ggcggacagt acacgcacgt cgactttgat cggtttggac ccaccaactc
    12661 gttgcacctc cattcgctgc acggcccgaa gaagcttggg ctgcaagctg atatcgaggt
    12721 ctccgatttc gtcgatgagc aacgttccac gatgcgcttg ctcgaatacc cccgcgcgac
    12781 tgctcgtggc ccccgtaaat gcccccttct catgaccaaa tagcgcggac tcgaggagcg
    12841 taggaggcgc agacatacaa tcgaatacga tgaaaggccc gttggctcgc ggtcccatct
    12901 cgtgcagcga ctcggccaaa acctctttac cacaaccagt ttcgccttcg atcaacacgg
    12961 gaacatccga tcccgcaagc cgctcgcata agggatacaa gcgacgcatc tcctcgctgc
    13021 ctcccacgac gcgaccaaaa gtccttcgct gagacagggc cacctccttg cccttttctt
    13081 cttgctgcac atgaaacacg gtctgtccca tgcgcacgaa ctcgccgcca tcgaggtaca
    13141 cttccacgat cgatattccg ttgacgaatg tgccattgct cgaacccaaa tcggtcaacc
    13201 gcaaacgccg atcgaccacg tcgaaagcag catgacgtcg agaaacggaa gggtcggtca
    13261 aacaaatctg gcagcttgcg ctttttccag tcagcgagcg tgaagggctg gatgaatcga
    13321 gaacgaactt tcttcccttg tccgggcctt cggccacgag caacaaaaag ctgaccgacg
    13381 aagcaagctt cgggcgtgca atcaagtggg ttcccgtatc tgcgaattcg gtcggacgac
    13441 gacccatggt ttcgaaccta gcatggtcag cagtggtttc gcacctgctg tgatgtatct
    13501 tatggcacca gtccctcaaa tagaccgaag ctcacggccg ttctacccga aaagcaagct
    13561 tggcctccag ttgtatcgtc ccctgggaag gttccggaaa tcgccaggtc gttgcctctt
    13621 ttttgacgca ctcgaccgtg ggcagatcga tcaaggcaat atcgtcttct tggacattcc
    13681 aaatctcacc aagaccgtcg atggtggcgc gtatggtcaa gcttcccgca agcttcggat
    13741 tttttcgcaa ccctgccgca taacagcgct ccaacgcctc ggttccccta tcgaccacct
    13801 ctcgaacctg ctccggattc ggtcgtgagg gactctccgg aatcaagcgc agcgagcgta
    13861 aaatgacccg cgcatcccgg caatcggaga tggaaggctc caccgcgggc agctttgtcg
    13921 ctaacccctg gctctcatac accttcacac acgcatcggc attgccttgc acatcgagtc
    13981 gacgaccttt gcactctcgt gcatacgctt cgcgagcgcg aacccgatcg acggtaacca
    14041 ccgaatttcc cagcgccaaa catcctcccg gttgtccgga agcacagcga ttcgctccct
    14101 gctgcgcctg tcgcgccctt tgctcgcgct gcatcacaca agcgtctcgc ttgcccttgt
    14161 ccgtcaattc gagaacacaa tactttcttc cgagttgctc ggagcgctgc gcgtcctcac
    14221 ccaaaaaggc atccgaagcc tctttgcaaa actgcgggtc gtccaactcg cagcctttcg
    14281 ccaacagcac ccgacgtcgt tccgcggcac gatcgacacg cgccggtggc gaaagctccg
    14341 gattgctttc cgatggaccc aataaaattc cacgaaccgc tccgaaacag gcttcctttc
    14401 ggcctgccag acaagatttt tcgtacatcg aaaccgatcg ctccgcgtcg gacacggaac
    14461 cgctcttcat gtaacgctga tcgtacgcac gcgcagccga tacacacgat ggtagatcgc
    14521 cattttcgca agcaatctcg cacgactcca ggttcttgtc ccggtcgttt gcacactttt
    14581 gcgcgggcga ggcgcagccc accatgccga gagcagccac gccaaggacg attcccgaag
    14641 ccttcattcg cccattcgac accgctagcg ccgccgggtc aacgcatcgc tcatcaacac
    14701 ccgcgtcacc acgatgtccg tgaaccaacg ctcgtgaatg aatccaccca ccaacctgaa
    14761 tcagcgacac gaatagctct tgggcaagca ttgttgcccc acgatctgat tgccctcggc
    14821 aatgtcaaaa caaccatagc cgctaggaca tccatccgga gccgtacact ccacaccaca
    14881 ggccttgccc gaagaaaacg caatgcaacg attgcccacc ccaggacagt ccgcatccgt
    14941 ggcgcacgat cgacaaagcg acgaaatatc cgcataggga ttgacatgcg ggttgtcatc
    15001 caacccatgc accccgtaat acacgttcca attcgaactc tggttaatct gccggaggat
    15061 ctgaagatac gtctgcgggt catgggtgcc cgcggacttc gccaaaagct gattgatgaa
    15121 gcccgacgtc gtatacggct gcatcgtgag ccacgaaaaa ttcacggtgg aaatgatgtc
    15181 gacgttggcg gtcgtcttcg ccggattctt catgatcgac tcgggataca ccccatacgt
    15241 tttgcatccg ttgaagacat acacctgata tttttccgga agactgatct tgctgagttc
    15301 atccgcgctg atcgcgtgac gtggattgta gtggtatacg agacccgaat aactggggtc
    15361 ttcccccgcg tgaccgtcgt aaatcaatac atccatggtt tgatacgctt ggatgatctt
    15421 cgcacgcagc ttatcgagat ccccaaccga tacgatactc gggtgaagca gcgtgatctc
    15481 cacatcgatt ttgcgacctt gcacatcgag taccgacgtg aacggcggtg aatcgagggc
    15541 aagatccgtg taacgggaag ccggatgcgc gtagcccgct cgcttcaacc actgaaaaat
    15601 ctcgtccgcc gctcgcaaat cataacgctc ttcgttgtaa tccccaccca ccaacaccaa
    15661 aatgtcgagc ttgccgtcct cgaacagacg gttgtattca gggaacgcat cgaatgactc
    15721 cttctcgggc tcgatagcca cctgaatctc ttcgagttca cccgaatagg tgctggcatt
    15781 gaagtccttc ggataactgc tgtacttgag ctggctttcc gtcaaaaccg gcagcttcac
    15841 acgaaacgtt ttttcccccg ccgaattggt ttcaatcggc agattcgaaa gcaacgctcg
    15901 cggaccgccc atctccagtt cccacaaaaa cgtgtacgac aaaccaagat cgtcgatggg
    15961 ctcgaccatc gaatccagcg acgacttgcg cacaagcccc gaaaatcccc catacccatg
    16021 gttggcgtcg tgactcgact tatccgtcac gtacacattg atgaaatgcg tatacgcctt
    16081 gaacttatac gctaacagtt gctcggcttg cgcccgcttc tgatcgtcgg tcttgtccag
    16141 ccagctttcg tccaagtcga gctgaccctc tccccaaagc cgatactcca tcgacgtcgg
    16201 cgaaagataa ttatccgcct tgcccggagg caccaccgtc gcttgccccg cttccgaccc
    16261 ctgcccttcc tcttgcacgg tcgaaccaca ggccaatacc aaccccacag gaataagcag
    16321 cgcaaaacga cggtccagca tggaacctcc catgaatcaa tgacagccac aaaagcacag
    16381 gaatgataga cgcgggaacg ccagggtcaa cggaaatggc caatgaaatt gtaataaaac
    16441 cagcgaattc gcttcgacag cggcaggact acagcaccga gaaaatttct tctatcggct
    16501 ttttcggaga ttgaatcgcc tcctgagccg gccttcccac cgaaatcacc gctcccaacc
    16561 tccagggagc ctcgatcccg acatcccgct cgatgctcgc cgaagcgact tgaggaaccg
    16621 tcagccagca cgccccataa cccatatccg tcgccgcaag cagcaagttc tcaatgctag
    16681 ctcccagact catcgcgtaa ggatgcccac gcaccgcatc agccgcttcg cgcgccagcc
    16741 ccgtcgtcat caacgcatca tccaaaaaac caggataaat tcgtgtagcc acgatgagca
    16801 acgccggcgc cctgccgaac aaccccacat aatcgaaaag tttcgatgtg gctttctgct
    16861 gcgcatcggt tttcggctca ggcaaaatcg tatgcagctt ctgctcgacc agcgaagcaa
    16921 tgcgctgaca tcgcgacgct tcgaccaccg catagtactt ccacggttga gcgttgttca
    16981 gactcggcgc tcgccccgca aggcgcacca tctctttcaa gtcatcgaca gacacaggtt
    17041 ccggcagata ggtgcgaaca ctggtccgcg cctccatcgc ttctttgagt tgcatcgaaa
    17101 aactcccgaa ctcccaccgc ggtgagagac tcattgcgtg atcagcttct gtgcataacc
    17161 cgcccagacg tcaatgcctc aacgcatctc accccttggg ccaatacctg agccgtgcta
    17221 gccgtggatg tcgacttgga ggaaacgtca tgaacatcgc catcatgggt tgtaaggaaa
    17281 tcaaagatga aacttgcatc ggttgccagc gctgcctgac cgctttccac cgacgggaag
    17341 gcgagttttc acgctacaaa gaccagcctg atgcgcgtat cgtcgccatt ttccattgcg
    17401 gaggctgccc cgccacttcc cccgtgctga gaatgaaaca attgcaaacc tggatgagcc
    17461 ccatgggcga gcgcgtcgat gtgctccacc tcggaacctg cgtgaaaaac cactgcgctt
    17521 acaaggatac cctggtttcg gccgtccgaa acaaagccgg tgtcgaggtc atcgaaggct
    17581 cccatcccta tctaccgaaa gatattttcg cctgaccacc gccccttttg cacgagaact
    17641 tggccaacga caggagaatt cgaaagcctc gtgcccatgg ccccttccga ccaacccgcg
    17701 tcatcaccct cccaatccac cgctaccccc tcagctccag gtggggattc tatatccgaa
    17761 aaacgcggaa aatgcccaga tcgccgacag ctccccgacc gacgcttctt tgctcggcca
    17821 gaaggacgcc gcaacaatca aggccggcga cgaagcgatt cttgcgattg ataggtaagt
    17881 atttgttatt tatcattaat ttttggctcc gccgatgaaa cggacgaccc gttctctcgt
    17941 atttgcccta agcgtcgccg ctttgctcgt gggagggttt ctccttttcc accgttcgca
    18001 gcggcagagg gttgcccacc aaccgctggc cgccgttcca gcagatgcac aattcatctt
    18061 gactgcgaac atggctcgcc tccgttcttc accctatcgg aacctgctcc gagacgcttc
    18121 catgtcgccc ttgacggctg gagcacagga atcttgcggc aaaaacctcg aggaacggat
    18181 cgaaaccttg gtcgtgtgga aacccgcgga accaggttcc accatgggca ttgcggccta
    18241 cgctcccgtc agcgcacaat ccgtgtggga atgcgcaaaa ggcaccatgg attcccgagg
    18301 agcccaagcc actttttccc atgtcgaagg atttcaaatc atcaccgacg aaaccctcgg
    18361 accaggctcc gcccagatcg ctgtccgaga cccaggattg cttgtggtcg cacgcccggg
    18421 aacccgtgcg cgcatgctcg atgccttcgc gggccgttgc ccctcggcat tcgaccaggg
    18481 aacgcacgct cgacttcgaa aagaactgcc caaaggagac gttgtcctca ccttgaccgt
    18541 caaccccgct tttcgccaac gggtcgccga ttgggtcgat gaaccatccg ctctcgacca
    18601 cgtaggggaa atcgggctat ccgcgattct tcaacccacc acggagctgc aagtcgtcgt
    18661 cgtctgtgaa tccgaccacg tttgccaatc cctcgaacaa cggcttcaag cgattcgaac
    18721 ccgtgcgcga aggtccatgc tgttgcggtc catgggggtt gccggctggc tcgatgctac
    18781 aaccctgcgg cagggaaacc aaagattgca ccttcacgcc tccctttccg ccgaacaagt
    18841 cctggatcta tggcaacgcc tctccaccga cccgcaagaa cccccgcctt ctttcgtccc
    18901 cgctctccga tcgtccagcg atgaaacgct ccgcccgccc agttcctccc caccataatc
    18961 aaccacgctc acccaccgcg cctcgatcga accttccatt gcgacaggca gaaggaagcg
    19021 ctacgggttc caaaaaggga aaagaccatg acgggcgtgt tgcagcatgt gggtgatgac
    19081 gctcccccgc ggtgtgttca ttgcggaaaa caagccgcgg gcccttgcgc ttcctgccat
    19141 cggatgattt gcgccgattg ctgcacgctc acggaatcaa gcgtaaaaaa atgggcaatc
    19201 tgcctggaat gcgaccggaa aaaaggacgc tccctcggcc acgcatgggg gtcgctgatt
    19261 ttatggctct tgctaccgct aggcatcctc gcggtagcca ccgccctcgt aggcttgctc
    19321 gcccacttat gagcgcggcc cacaccatcc gggtccgcga cggccagaac gctcgttcct
    19381 tcggccacga tctactgaat atccatgttt tcgtcttttc gaccgctatc ttcccggtcg
    19441 caaccgttgc ccatagtttc ggaggaaaaa gctttccgca cctatccacc gataacgatc
    19501 cgagcataag tccaccttgc tttcccaaag tctgaacaac agtccccttt ccagttgttc
    19561 agaggatcct aacgatagcg gggacgacgt cccgggtacg aagagcacgt tggtcctcgg
    19621 gttgtcgatg gtgggcgtgt cgtgcatgcc ttcggttctc ggagccgaga cgcttgcgcg
    19681 aaagcatgat aactaattgt ttttattata aaatacaagc tatcgccgtt tcgataccca
    19741 agggttgggc cagcgtcgga gtagaattga gtcatggctg ttgggtggtc agcccatcga
    19801 tgagcggaat gtttcatggt gatgaggaga tgatgctggc agaaaacaat tctgctattc
    19861 ttctccaatt cccgttcgag gaggcgttct atgcgatttt tccccttttc ttattatcgg
    19921 tttatggtag cggggacctt gctgttcgcg ctctccgatt gctctggcga cgagtttacc
    19981 caagaggcgg cggatgcatc ggtggactct ggcggtccac aagaggcttc gaccggtggc
    20041 agtgggggaa cctccacagg cggagcgggt ggagcgacgg gcggcagcgg gggagcgccc
    20101 acgggtggtg cgggcggagc gacgggcggc actggagcaa gcgcggggca tgccggaact
    20161 ggtggcgacg ctggccccga gcctacttgt gacccgaacg aatcaccacc gaccgacgcc
    20221 atttacgtga gcgcgaaaga aggcgacgac aatgcaagcc aggggagtcc ggaacagccg
    20281 ctgcaatcct tgagcaatgc catcaaattg gcagtccata accagattgg caaaatcatc
    20341 ctggaccaag ggacctacaa ggaggcggtg gtcctttccc tacaagatcc gctggtcatc
    20401 atgggaggtt ggataaaggt aggaaccgcg tggacacggg attgcagcca gacagcgcgg
    20461 aacaaaacgc tgttgcactc acccacctcg acagggctcc agatcacggg caattcctcc
    20521 gtttcgctac aaaccatggt gatccaatcc accgggacca ataacgcggc gcccgataac
    20581 caaagtggag agtcgtgtta cggggtgttc gtgtcggggg ccgagctatc gatgaacgat
    20641 gtcaccattc gcgcttgcgg gggccaagac ggaggaaagg ctaataaata cgaagaggta
    20701 cagccgcccg attgcagtca actgcaggga accggatgct cgatgggaga acacggaggt
    20761 tctgggagcg gcggacagga cgctaaggag gccggaacat ttacattaaa tgggtatacg
    20821 cctgcaaacg gacagcatgg aaactatggc agtaatggaa acaacggtac accaggcaac
    20881 cctggcaata ccgcgcaatg ttatgaaaaa ggatgcactg ggcagcaagt gtccggctct
    20941 tgccctagta atcattattg cggtaacgaa acgccacaga aagatacctc ctcacaaccg
    21001 ggttcctgcg gttgcggagg tcgtggagga agcggaggcg cgggcggaag gggcggcggc
    21061 ggatccttcg gaatttatgc cacgggaaat gcttccgtcc ttgtaaaaaa ttcgagggta
    21121 gaagcggcat caggtggaaa cggcagtcca ggacaaaccg ggcaaattgg cttaaatgga
    21181 ttagatggca agcaaggcga tagcgtaatg tgctatacgc agtgcaaacc catgcaggtt
    21241 ggcgagaaat gtaattgcga acaacccggc tcctatcaac tggctggcgg tgaaaaagga
    21301 gggaaaggcg gcaacggagg caatggcggc cctggaggcg caggctccgg tggcccttcg
    21361 attgccgcgc tcatccatgg tagcgccacc ataagccatt taaacgtgga ttttctgcat
    21421 tcgacggccg gcaccccggg ttctccctct gcagcccaag gagatgccaa ggacatcctt
    21481 gaagtgcagt aagactccag cgaatacccc aaagacccca acacgtcgcc ttttcgatcc
    21541 cccaccccct cgaaatcacg ttgccgtgat gctcgtagcc actggacggc atctcgaaac
    21601 gatacgtccg acttgtcgca cgagccgtaa gcatccacgc tcccgccatg ctctccactt
    21661 cccaccgaat gcgatggctc gtggaccttc acggccgtcg ccacgtggtc gatgcctctt
    21721 ggcccggctt ttccaactgg attgcggtcg atggtcatac ggtcgagcga tggcgctggc
    21781 ccagcaataa tttatggaca tggcgcacct tcccgctcgc tggatcgtcg gcaaggatca
    21841 tacgacgacg ttcgggctta tttagtttcg cgtacgaatt gcaggtcgat gccccagggg
    21901 cgcacgtgca acaagtggat ccgtggtcat cgctttggtg gaacgggcgg gtttgaaacc
    21961 cgcccctacg gtatcgccga tcgattccta cctcattgcg aaccgatcct ttccagcagt
    22021 tcgaccgccg ttttttcaat caatcgacta cgacactccc gggcaaggat tcgcgtgcgc
    22081 tccagttctt cccgcgccag gtcggattgc cccagccgat aagctagcgc cccaagcaga
    22141 aaacgaccgt cgagcgcatc ccaagtcagc ccttcgtctt ccgcttttcg cacgagaaga
    22201 cgcaaggatt cgatcgcgtg ttctgctcct tgacgacctt cgagatacca caagtacatg
    22261 cggtcgtgac gggccagccg gtcgtagcca tattgttccg caatcgcgag ggaacgttga
    22321 aacgccgtat gagacctggc gaaatcttcc acccacaacc acgaatcccc aagattatgc
    22381 aatcccaacg cggtttcgta cgacaggcca atcgattgcg ccaggtccgc tccccgcgcc
    22441 gaagccatcg ccgcttcctt gaaatcccct gagaaatgga agatcagcgc ccgcaacttc
    22501 tcccgatcca tcgcggcacc cacatcccca ggctccgaaa tcgccgtcgc tcgctccaga
    22561 tactccaacg cccgcttttt ctgaccggta gccccatacg cttgtgccaa ccccatgtca
    22621 taccgaacgg cgtccttgcc ttcgaaaagc cggtcgccca tctgctcata gatcgtgacc
    22681 gctgcaccaa attcaccacg gattccgaat agttgcgctt gtgttagcaa ggtcagggag
    22741 cggatgggct cgttcgaacc cgccagttcc aacgcctcat ccaacagcac catggctgca
    22801 tcgaagcgat gcgcgtacgt aagcatgctc gcgccatcga cccgcgccat caccctttgc
    22861 gacaacgtcg cctccttgtc cacacgcgcc acgactcgtt ggagtacatc gagcaacgaa
    22921 gctcctgcgc gcacgcgacg cacgacatcc cgcaattccg ccaaccactg cagcaaggta
    22981 tccacgggac aatccgcgag agatacgaga tcgaaggcgc ggaggtaatc cgaaagcgca
    23041 ccttcgagtt gccccgagcg tttgcgatac ccagccgaca acgcgaacaa tcgcgcagct
    23101 cgatgcttct ctcccgcttc caaaagatgg tagcccaccc gatccgccag cgcatcgacc
    23161 tgttctcgcc ccacggcggg gattgcctgc tcaac
//
LOCUS       DAOSOR010000019        22820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_2278_length_22820_cov_4.031408, whole genome shotgun sequence.
ACCESSION   DAOSOR010000019 DAOSOR010000000
VERSION     DAOSOR010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 22820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22820
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2278_length_22820_cov_4.031408"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2111
                     /locus_tag="PK710_01915"
     CDS             <1..2111
                     /locus_tag="PK710_01915"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT08490.1"
                     /translation="SELMRESGPHPLLVLLVTRPEERVLPFIENLVRIELRGLRFDEM
                     AQLVESRFGVQQGVASACADLIPRVAGNPFFLLEMVDALLEQETLEIKDHDGRQIMVR
                     AKQSANRAQALPSTLEQLIANRLNELPPEERAVVSWLSAAGGPLSESDIVNLGRLTSP
                     EPIGRLCARGMCDRRGDSIDFRHPIARDVAYQAIDAKERVLLHRRLGEHLAETPIASG
                     LSAVIVARHLARGKLFSRAADYYLEAAQAARAGYQTQLARPYFQRALLLLDTSDLRRL
                     RAHEALEGIYRLLGRHKQRRRHLHALRQMARGSNSAEWVALALLRTAVFYYDENFYSS
                     CETVARQACEAARVASAPYIEVEALSTQGEALRELGDVQGALAACDRALKLAKNKGVS
                     PRIRADVLRSRGLLLRRVGRVREAVDCHVEAIAVFRRVGARRPEARTMNALAFAMFVL
                     ERFEDSIALALSSISIDLAIGGRFQIAKTLSNIGQAYARLGDLARGIAYLKRAREAHE
                     HYGDQDSRADTLIVSAEVMLEAGDLDAARSFCLDAAALTSVTGSAYDEVHEKIVHAML
                     DRMSNDSARAVTLAASARQAAESQALVSFSCLATAVEAAARVDIGEVHTGILLATTAL
                     GSIAAIQGSEFGLEICVICWDTLRRAGSPQAIEAKRVTLARIAQIVSHIRDPRLKSLF
                     FNRSVVRLVLDEAKPQTSVS"
     gene            2178..2927
                     /gene="thpR"
                     /locus_tag="PK710_01920"
     CDS             2178..2927
                     /gene="thpR"
                     /locus_tag="PK710_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOT08491.1"
                     /translation="MLDTVRAFVALNLEMASTRRIIALQRGLRASPELPSAQVAWVAA
                     PNLHLGLRALGSMDAALTPALADGMREALRTLPPIRIPLVGPAAYPSESEARLVVVEP
                     AQIPDALRNLGERIEALVQSIGFAPETRPFRPHIVLARTSTAVAVSRWFASLGRVDLP
                     EAQATECVVFEQKAVPAAEYPALIRVGLSNPTSHRSQRPRPSQRSRARSKPPAAGAQD
                     SSTTIPSPPKVPSDLDISPGSDVDAVKSDDA"
     gene            2920..4011
                     /locus_tag="PK710_01925"
     CDS             2920..4011
                     /locus_tag="PK710_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOT08492.1"
                     /translation="MHDMGSDGSAMGQQGGGFDEQLVASAELLQRIRGLSRRFSGVGL
                     RGTALIRMPQCDRLVSSEARVWFALENLQITGSFKVRGALAALDSWSGCTDRTVVTAS
                     AGNHGAGVAFAAEVLGWQAVVVVPEATPRRKVQAIQRAGVKLERVQGGYDRAEEQAYS
                     LAKTHGWRFLSPYDDVVVAAGNGGSLGFEIVQQLGCVPERVVVPVGGGGLASGLGWAL
                     AQQAGESIGQNRRVWGVQSEMSPAMARSLEEKRAIESLEPLGPTLAEGLEGGVRARAF
                     DRAAMVLGGVWVVTEQEISWAIQWIWQELGLRVEGSAAAALVPLLSGRLRGWIQGNMV
                     IVLTGRNVDDDVFRQVTGDACCRSVGWSL"
     gene            4061..6007
                     /locus_tag="PK710_01930"
     CDS             4061..6007
                     /locus_tag="PK710_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HOT08493.1"
                     /translation="MRNRTKGDVRLLDQMLMDQRISTEQYEASLTHYQANGGRIEESM
                     LEVNAISEVDLLKYLAAFHKTRFVSTEKLSKADIHRTTLEKVPKKLAEGLGVFPVLWD
                     PQAGVLSVVTSDPDNLEVLKEVQIFAAAKEVRAFVARPAAVRAAIAKAYGGDIHAFAI
                     LDRSAHEQFQTMLDVYERNLVSEETMAVALADARERERVLSGDALEQSASVAVGGLGT
                     VTSEPYIETLNVMVSLIENPRPDLRGHSSHVARLTKRIAERIGLAPVEVASMVVAAHL
                     HDLGKMSAYHLTALNVSQYEGHRVAAEKSYMTPRRLLEGVNLNPSTIKALESMYERFD
                     GTGLPGVLGGKDIPLGARILAITDTYADLTQNPRNPFRKTLRPMEASEVLERFKTTVF
                     DPNLVDLFKHAVTGDDMRARLLANRHKALVIDPDPEETTLLELRMIEQGFEVVICRAS
                     DQAAKALHSGDFEIVVSEVDLKPEDGFALFGAMKEQPYGQDLSWVFLTRRAARGDVQR
                     GFDLGAADYVIKPAQADVLVTKLKQIIERNATKTRHKGVSGSLTEMALPDIVQILWHG
                     RKTGALRIRSGSDHGEIHFQHGAIVNAMHGRLRGEDAFYTMLRLKSGEFALDPNFIPQ
                     AKVIHDSPEALLLEGMRRLDEGQG"
     gene            6094..6855
                     /locus_tag="PK710_01935"
     CDS             6094..6855
                     /locus_tag="PK710_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08494.1"
                     /translation="MSKAQSPLLGFNNNVKHRGRMFHIQTEDSGIKYPHIITHLFADG
                     GRILKSTKTSYAEYIGSENLRETVRNLMQEQHKSMFIALRDGQFDHLIDETMDEKRGS
                     GEQGKGAAEPEPSVAVPESAQGPISSAVLDVEVLERAAASVQSRSPLFGGEDMPPPPP
                     TVLPTQKPSGSYRAVGVGPSSQATDKNKGHYSAPRPASIFGTASVSESSKTVFGEDVI
                     SSKSLDEVIMDFLSEEFDTSSNHEGDEDSDKRGRS"
     gene            6824..8539
                     /locus_tag="PK710_01940"
     CDS             6824..8539
                     /locus_tag="PK710_01940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT08495.1"
                     /translation="MKTRTNVDARNREKDFLARAGALAWALPTDSWLYEDDCLVVVSK
                     PAGMSVSGSEHDLLSRVRIILDFQAKSTEGLGAPIHLDRDVSGVVALAASKQANASMS
                     RQVQQHALSWTFVVAVSCSFDLAPRGQRDVGVIRDRNGLMRASRGKSDKRVHLDYQVQ
                     SRQGDRYLIEVRCADGPRAIRAVLASMGIAVAGDVVFKGPEASRLLLHAKQLTLLHPR
                     HEGVVTYQAPEPWAFHAWMHRQQRAEQLDTSSLAQALKEAACKRFSLCARGLEAFRHV
                     HGEAEGLRGLDIEWYGNHAVVWVEEQTCDRAVDGLLAVLGEWEPAGIYLKKRPKQASR
                     ASDGQGAPLVFSHAVRGQNTPEPFSILEDGLEYLVDLGQGLSTGLFLDMRRNRAWVRQ
                     NSHGAEVLNLFSYTCSFTVAAAAGGAKRTVSVDISKRSLEVGDRNLLKNGLKSPNHEF
                     VCDDVRRWVERANRHPHRFDMIIFDPPSFGTSRWGRFSVMRDYESLVSLTMRLLRDGG
                     WLLACTNHRAVRMGKLQGWLQSAASAAGCRWTVLERASADLDFPVGLEGEPHLKAFRC
                     RVAWK"
     gene            8685..9110
                     /locus_tag="PK710_01945"
     CDS             8685..9110
                     /locus_tag="PK710_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08496.1"
                     /translation="MIRTALLALIVGTLATGCVVTTGGAPSNSSPTHSYKVRGKSRSH
                     SNHGGSRAAATPGTTQPGGQSGGGEAVATPPVVTPTTEATPTTGTTPTPGMTTTQGLP
                     SVHQQSNPAGTTTTGEEQNGNQPRRRLPKANLPVLTPTK"
     gene            9202..10950
                     /locus_tag="PK710_01950"
     CDS             9202..10950
                     /locus_tag="PK710_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT08497.1"
                     /translation="MSFHARQLTAAAIVLGISIVLGFLIPTAATYLMLVGALGAVAVF
                     ATSLGVNPTGLRELPTAIAQLERGERPQLPDNASPYVVQAFEKASTLADRARDDGLGS
                     TLEALGSALREASEGNKPSWPEDLPVNTRPLWKQLAQIASSIETSQQSIASRATALQR
                     SSSKVDEVVAILTNGLADRTKLVTETSRTVVDVSGHARDLGRHVEALAATADSSASSI
                     VQMTITNNEVATSVGDLAMSVRDTASSIEQMAYSIKEVAKNVDALSLTTEETSSSMNE
                     MDVSIDQVQSNANETARLSEEVAQDAERGAEAILKTIGEIYRIKESTGEAVSVISNLG
                     SKIEAIGQILNVIDDVAEQTNLLALNAAIIAAQAGEHGKGFAVVADEIKDLAERAGAS
                     TKEIAELTKTIQNESKNAVIAVERGAKNVDRGVEVSNEAEHALKKILESSQKSTNMVR
                     AIARATVEQAKGSRQVTDAIGRIAETVQQIAVATAEQARGSELIMRSVDRMRAITQQV
                     EQSSQDQAKGGRDVTASMESITSLLEQLSHAHRALSRGADSLVASATQWEQAIEQEES
                     ALHQLSGETLRLRDLR"
     gene            complement(11129..12580)
                     /gene="dnaA"
                     /locus_tag="PK710_01955"
     CDS             complement(11129..12580)
                     /gene="dnaA"
                     /locus_tag="PK710_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOT08498.1"
                     /translation="MDKSGDINLITVTSRENVIERIHSQVWEDAIAKVREHSPASFDT
                     WFAGVQFDGLVDGKLHLRARDEFVRDWIRDHFQPALLHIIEQTTGSSLDVVWTIDSDL
                     DSPVSSKRIPDPVIPRAIQIVEPEPQPVIRESKRPASTAALLGVNPKNTFDNFVVGPS
                     NQLAFAAASTASGGAGRRYNPLFLCGSTGLGKTHLLHAIALRIRSERPSARIAYGSAD
                     SFTSEYINSISDRRMDEFRARYHQCDALLIDDVQSLAGRTQTQEEFFNTFNFLHACDK
                     QIVLAADKYPQQLDRMEQRLVSRFTWGLVADVQTPEFETRVAIVRKKAKIENVDLPEE
                     VAVLLAQTVQSNVRELEGMLIRLIAKSSLLGCPLDLSFAEHELSSAQPTRKPTPTVEY
                     IQQVVCRHFHVETSDMLSKARHRSVSRARHIAMFLCRQRLKCSFPEIGRAFANRDHTT
                     VMSAVRKVEAMRDHDPELRAHLEEIERRLSSGE"
     gene            13276..14244
                     /locus_tag="PK710_01960"
     CDS             13276..14244
                     /locus_tag="PK710_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08499.1"
                     /translation="MPRIRVLALTATLFIFAGTIPNAHADRSRLPPEIGYNYGEVEDA
                     RWAATSGALRAVGNGITGIWGNPASLAQAQVYHIGAIAGVWPEAKRQSYGVGAMDSVT
                     SRLAAGLGFVWSDQDPDGLKRESRDLRLALAYPFSPAVSFGLTGRYLHLKQDGLGPLG
                     QSYASGGLADEAMLEGFSFDAGLRIAPSENFSFGLLGSNLSNPGHGLQPTTAGGGLGV
                     GTRDFVIEADVLADFTTWQKTTTRAMAGAEFLAGNNFPLRLGYRYDAGASSHAISGGL
                     GYIDPAFSVELGARRSVAGDGHTVLVFTVQYFVESSGMTRAPSAAF"
     gene            14641..15384
                     /locus_tag="PK710_01965"
     CDS             14641..15384
                     /locus_tag="PK710_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08500.1"
                     /translation="MERLGLSAATRAVILVGDYGSGKTEIAVNLALQLAATQAYPIAI
                     ADLDLVNPYFRCREAVEPLERVGIRVVIPQGGHRFADLPILLPQVKGLLQNDDQLAVL
                     DVGGDEVGSRVLAGLVKAYRQGVHELWFVVNANRPFNDTVQGCAATISRIEGASGLRV
                     TGLVPNTHLMQETTAEMVGEGIVLADQVAERLSVPVRMVAVMESMADAVRAHHPVLRM
                     RRLMVPPWLRRDTVQLGRTGPEAFRLRST"
     gene            15397..15648
                     /locus_tag="PK710_01970"
     CDS             15397..15648
                     /locus_tag="PK710_01970"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT08501.1"
                     /translation="MPSIQVDRDRCKGCELCNDACPQGILGMSKQINIKGYFYAEVID
                     PGRCIGCMLCAVMCPDLAIAVHGTAVQYRLYDYFQPEQR"
     gene            15658..16719
                     /gene="vorB"
                     /locus_tag="PK710_01975"
     CDS             15658..16719
                     /gene="vorB"
                     /locus_tag="PK710_01975"
                     /inference="COORDINATES: protein motif:HMM:NF005507.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOT08502.1"
                     /translation="MAKKLMKGNEAIGEAAIQAGCRNYFAYPITPQSEVAEYLSRRMP
                     EVQGHFVQAESELGAANMVFGAAATGELVFTTSSSPGVSLMSETISYIAGAQLPAVFV
                     NIVRGGPGLGGILPAQSDYFQATKGGGHGDYRLLVLAPATVQEAVDLTMLAFQLAFKY
                     RNPVMILGDGMIGQMMEPVEFKPPPPAADLPSVETWATTGAKGRKCNIAHSLFLDPEA
                     LEKNNHVLAAKYKVWQKDEIRFATYNLDNNPRLLIVAYGTMARICSTAIDELKQKGVE
                     VGLIRPITLYPFPAAAILQHAKKIGKVISVEMSMGQMVEDVQAAVGHDIHVGFFGRAG
                     GIIPSPDEVVQALEAELRA"
     gene            16734..17528
                     /locus_tag="PK710_01980"
     CDS             16734..17528
                     /locus_tag="PK710_01980"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOT08503.1"
                     /translation="MTTLFAPPVSMNERATHYCPGCTHGVIHRLVGETIDELGIAERT
                     VGIAPVGCSVLIYNYYNIDFQEAPHGRAPALATGAKRARPDLMVFTYQGDGDLASIGT
                     NEIVHAANRGEKITTVFVNNAIYGMTGGQMAPTSMPGQVTTTSPRGRDPALAGFPMRM
                     CELLSTLRTPAYIVRASVHTPQLAVQTKQAIRRAFQLQMENRCFSFVEVLSTCPTNWG
                     IPPVKALRWLQDNMLPYYPLGVFKDPDQQERADRQVPTPWRDNDAK"
     gene            17518..18072
                     /locus_tag="PK710_01985"
     CDS             17518..18072
                     /locus_tag="PK710_01985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOT08504.1"
                     /translation="MQNEVMFAGFGGQGIMLIGQMLAYAGMGEGKNVVWIPSYGPEMR
                     GGTAYCTVVVSDKDIGSPIIDRPESIAVLNRPSLDKFHTRVRGNGLLIINTSLIDATT
                     DRSDIDVLNVPANQIAMEAGTGKAANMCVLGAYVGRTKAVTLESLRGLVRYQFKSKPQ
                     FIDVNLRVLEKGYELGEQLATKNN"
     gene            18091..18579
                     /locus_tag="PK710_01990"
     CDS             18091..18579
                     /locus_tag="PK710_01990"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOT08505.1"
                     /translation="MTQPVDMQTTEDVLARYPSDPRHLISVLQDIQLAYRYLPKEAIA
                     RVSAALGVPLAKVMAVATFYKAFSLTPRGKKILRVCTGTACHIRGAPLLLTEIEKRLS
                     IKPGDTTQDLEFSLEAVNCVGACALAPVVVVNDTAHGNLSVTKVRRLLKPAEKSTEKE
                     AN"
     gene            18579..20468
                     /locus_tag="PK710_01995"
     CDS             18579..20468
                     /locus_tag="PK710_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOT08506.1"
                     /translation="MDTNLRYQSIEAFRSHVAALHDSKLANQRRVLVCCGTGCLAGGA
                     AQVAEAMAQQLAAIEPEKSAQLQLVMKKTGCHGFCEKGPLVVLLPQGTFYKGVKAKDV
                     PEIIKESILEDRVVERLLYVESSTGERVEKYGDIEFYAKQTRVAMRNIGLIDPASLDD
                     YLLRGGYVALAKALSSMTPEQVMTEVSDAKLRGRGGGGFDAGRKWRSCKNSPADERFV
                     ICNGDEGDPGAFMDRSIMEGDPHGVLEGMILGGYALECKQGYIYVRQEYPLAVENLQL
                     AIDEARARGLLGDNILGTGFSFDIELSRGGGAFVCGESSALMRSVEGKVGEPRAKYVH
                     ATDKGLWDKPTVLNNVETWVCVPRIIEQGAQWFASIGTERSKGTKAFALTGKVKNTGL
                     VEVEMGTTLRQIIFGAGGGMLNDGPRGKFKAVQTGGPSGGCLPEDMLDLAVDFDTLTK
                     AGSMMGSGGMIVMDHSTCMVDVARYFLSFLVDESCGKCVPCREGLFQMMHIVSRICEG
                     QGTMEDLNKIEDLATAVELGSLCALGKSGPNPVRSTLRYFRQEYEAHIRDKKCPAGVC
                     KALISYTIDHDKCDGCGACLRACSSLAIAGTIKKGDTFQIDQLQCDRCGACETVCKRG
                     AISAA"
     gene            20492..21286
                     /locus_tag="PK710_02000"
     CDS             20492..21286
                     /locus_tag="PK710_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOT08507.1"
                     /translation="MAFFMTINGKRVEANDGEMVLGAARRAGIAIPTLCHHEAVEPFG
                     SCRLCMVEVTKPEWKGWKGLMTACLYPCAPDLIVETDSERVHQVRRNVLDLLLARCPD
                     AELIQKLAAEYGVHQTSFMQREKPDKCILCGLCVRICETAATSAISTVQRGHDREIGT
                     PWGEPPPDCIGCLACAHVCPTGHIQYEETGMTRMIWGRRFELQRCEKTGKPLPITKEQ
                     AAFLAKRQNMNPNYLVTSADANRKQTAETFARIARWNKLGMQQEVK"
     gene            21286..21705
                     /locus_tag="PK710_02005"
     CDS             21286..21705
                     /locus_tag="PK710_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOT08508.1"
                     /translation="MPQRLVVQPQYCIGCRACEMACAFTHGQQGQPGKSRCMTLTMAV
                     KDEYVPMLCLQCDNAACVQVCPVFAISRNEQTGVVHVDMDKCMLCMACTVACPFGNMH
                     FDAPNRAVVKCDLCAGYGDNPRCAMFCPTKCLSVEQL"
     gene            complement(21721..22542)
                     /locus_tag="PK710_02010"
     CDS             complement(21721..22542)
                     /locus_tag="PK710_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08509.1"
                     /translation="MISRRHWMKLAGLSLLAAGCEAIGLKATVVTSRTVNGQTTTTVR
                     EARNWEEFEQAMGEVATDFSDFAKEVGSTTAELAKKLVDVPPQGQVQLGQLSPSLAPY
                     QGDVRYDYLKVARMNPNAKYDFKYVQIGMPEYDNFFRASAEMYAAAYQLTETGRHVYL
                     ASYNAQGQEAPSDVQSGRKPIRKDEIEALTSQLGESESAKRLTTLWEAMAELGGQLAA
                     KAGETAQTGMALVASAPSQILNPKLVLHIDLIVKGLEQSVSLVKDTGSLLGKMVG"
     gene            complement(22595..>22820)
                     /locus_tag="PK710_02015"
     CDS             complement(22595..>22820)
                     /locus_tag="PK710_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HOT08510.1"
                     /translation="GAVGYVYNGSGIEGRPDWSIGFVYLPALAGIVLVSVLTAPLGVR
                     LAHALPVDKLKKVFAVLLYVVAIRMLFSLF"
BASE COUNT         4708 a         5855 c         7258 g         4999 t
ORIGIN      
        1 tctcggagtt gatgcgggaa tcggggccgc atccgctctt ggtgttgctc gttacgcgcc
       61 cggaagagcg agtacttcct ttcatcgaga acttggttcg catcgaactt cgtggactgc
      121 gcttcgatga aatggcgcag ttggtggaaa gccgttttgg cgtacaacaa ggggttgcat
      181 cggcttgtgc ggatctcatt ccgcgtgtgg cgggaaatcc attttttcta ttggaaatgg
      241 tcgatgcgct gctcgagcaa gaaacgcttg aaatcaagga tcatgatggc cgacagatca
      301 tggtgcgggc gaagcaatcc gcgaaccggg cccaagcgtt accctccacg ctcgagcaac
      361 tcattgccaa ccggctcaac gaacttccgc cggaagagcg agcggtcgtt tcctggcttt
      421 ccgcagccgg aggccccctt tcggaatccg acatcgtcaa tctcggtcgt ctgaccagtc
      481 cagagccgat aggacggtta tgcgcgcgag gcatgtgcga ccggcgtggc gattcgatcg
      541 actttcgtca tcccatcgcc agagacgtgg cgtatcaagc catcgacgcg aaagagcggg
      601 ttcttttgca tcgtcgtttg ggtgagcatt tggcggaaac gccgattgct tcgggattgt
      661 ccgcggtgat cgtggcgagg catctggcgc gaggcaagct tttctctcgt gcggcggact
      721 attacttgga agcggcacaa gcggcacgag ccggatacca gacccaactc gcaagaccgt
      781 attttcagcg cgctctgcta ttgctcgaca cctcggattt gcgtcgtttg cgggcccatg
      841 aagcgttgga gggcatttat cgattgctgg gacgacacaa gcagcgtcga aggcatttgc
      901 atgctcttcg gcaaatggcg cgagggagca acagtgcgga gtgggtggct ttggcgctgc
      961 ttcggacggc ggtattttat tatgacgaaa acttttattc gagttgtgag acggtagctc
     1021 gccaagcgtg tgaggcggcg cgggtagcgt cggcgcccta tatcgaggtg gaggcgttat
     1081 cgacgcaagg ggaggcgttg cgggaacttg gcgacgtgca aggggccttg gctgcctgcg
     1141 accgagcgtt gaaactagcg aaaaataaag gggtttctcc gcgaattcga gctgatgtcc
     1201 ttcgctcccg tggcttgttg ttgcgtcgcg tgggccgtgt tcgcgaggcg gtggactgcc
     1261 atgtggaagc gattgcggtg tttcggcggg ttggggctcg aaggccggaa gcgcggacga
     1321 tgaacgcgct tgcttttgcg atgttcgttc tcgagcgttt tgaggattcg attgcgcttg
     1381 cgctgtcttc gatcagcatc gaccttgcga tcggaggtcg atttcagata gccaaaacgt
     1441 tgtcgaacat cggccaagcc tatgcgcgac taggagattt ggcgcggggc atcgcttatc
     1501 tcaaacgggc tcgtgaagcg catgagcatt atggggacca ggactctcgg gcggatacgc
     1561 tcattgtatc ggcggaagtg atgctggaag cgggggactt ggatgcggcg cggagtttct
     1621 gcctcgatgc ggcggcgctg acttccgtaa cgggcagtgc gtacgacgag gttcatgaaa
     1681 aaatcgtgca tgcgatgctc gatcgaatga gcaacgattc ggcaagagcg gtaacgcttg
     1741 ccgcttctgc acgacaagcg gcggaaagcc aggctttggt gagtttttct tgtcttgcca
     1801 ccgccgtgga agccgccgcg agggtcgata ttggggaggt tcatacgggg attttgcttg
     1861 cgacgacggc gctcggttcc attgcggcca ttcagggttc cgagtttggt ttggaaatct
     1921 gcgtgatatg ttgggataca ctacgtcgtg cgggctctcc ccaagccatc gaggcaaagc
     1981 gggtaacgtt ggccaggatc gcgcaaatcg tatcgcatat tcgagatccg aggctcaaaa
     2041 gcctgttttt caaccggtcc gtggttcggt tggttcttga tgaagccaaa ccgcagacct
     2101 ccgtgtcgtg agcgtctttg gctcaatcct accatgccct gatggaatta cgcttcgagg
     2161 cgtctatact cgccgccatg cttgatacgg tgcgagcctt cgtggctctc aatctcgaaa
     2221 tggcctccac gcggcgcatc atcgcgttgc aacgagggtt gcgcgcgagc ccggagcttc
     2281 cttccgccca ggtagcgtgg gtggccgcgc ccaacctgca tctaggtctt cgagcgttgg
     2341 gttccatgga tgcagcgttg acgcctgccc ttgcggatgg gatgcgtgag gcgcttcgga
     2401 ccctgccgcc gatccgtatt ccgctggtgg gtccggccgc gtatccttcg gaatcggaag
     2461 cgcgtctggt ggtggtggaa cccgcgcaga ttcccgatgc gcttcgaaac ctcggggaac
     2521 gaatcgaggc tcttgtgcag tctatcggat ttgctcctga aacaagaccc tttcgtccgc
     2581 atatcgtatt ggcgaggacg tccacggccg tggctgtgtc gcgatggttt gcttcgttgg
     2641 ggcgcgtgga ccttccggag gctcaagcca cggaatgcgt cgttttcgag caaaaggcgg
     2701 ttccagccgc ggaatacccc gcattgataa gagtggggtt gtcgaatccc acatcccatc
     2761 gttcccaacg acctcgcccc tctcaacgtt cacgggctcg ctccaaacca ccggcggcag
     2821 gggcccagga ctcgtccacc acgattccat ctcctccgaa agtgccgagc gatctcgaca
     2881 tctccccggg gtctgacgtg gatgcggtga aaagtgacga tgcatgacat gggcagtgac
     2941 ggcagtgcca tgggccagca agggggaggt ttcgacgagc agcttgtggc ttcggcggag
     3001 ctactgcagc gaatacgcgg gttgagtcga cgtttctctg gggtggggct taggggcacg
     3061 gcgttgattc ggatgccgca gtgcgaccgg ttggtatcgt cggaggcgag agtctggttt
     3121 gcgttggaga atctacaaat cacgggttct ttcaaagtgc ggggggcttt ggcggcgttg
     3181 gactcatggt ctgggtgtac ggatcgaacc gtggtgacgg catcggctgg aaatcatggt
     3241 gccggggtag cgtttgcggc ggaggtgttg ggttggcaag cggtggtggt ggtaccggag
     3301 gcaacaccac gtcggaaagt gcaggcgata cagcgggcgg gggtgaagct ggagcgggtg
     3361 caagggggat acgatcgagc ggaggaacag gcatattctc tagcaaaaac gcatggttgg
     3421 cgttttctat ccccctacga cgacgttgtc gttgccgcgg gcaatggggg gtcattggga
     3481 ttcgagatcg tgcaacagct tggttgtgtg ccggagcggg tcgtggttcc cgtgggtggc
     3541 ggcggattgg catcggggct tggttgggcg ttggcgcaac aagcgggcga gtcgattgga
     3601 cagaaccgtc gagtgtgggg agtgcagagt gagatgtctc ccgccatggc gaggtcttta
     3661 gaggaaaagc gagcgattga atcgctcgaa ccgttggggc cgacgcttgc ggaggggttg
     3721 gaaggtgggg ttcgtgcaag ggcttttgac cgggcggcga tggttttggg gggggtatgg
     3781 gtggtgacgg aacaggaaat atcgtgggca atacaatgga tttggcagga attggggctt
     3841 cgggtcgagg ggtcggccgc cgcggcgttg gttccccttc tttcgggtag actacgaggg
     3901 tggatacagg gaaatatggt catcgtcttg actggaagaa acgtcgacga cgatgtattt
     3961 cgtcaagtga cgggcgacgc ttgttgtcgt tccgttggat ggtcgctttg aattatgaaa
     4021 cgatacaggg ggtcactcga ttggggtgac aggtaggttg atgcgaaaca ggaccaaggg
     4081 tgacgtaagg ctgctcgatc agatgctgat ggatcaacgc atcagcaccg agcagtatga
     4141 agcgtcgcta acgcattacc aggccaatgg tggacggatc gaggagtcga tgctcgaggt
     4201 caacgccatt agcgaggtgg atttgctcaa gtaccttgcg gcgtttcaca agacgcgatt
     4261 cgtatcgacg gaaaagctat cgaaggcgga tattcatcgt acgacgttgg agaaggttcc
     4321 gaagaaactg gcggaggggc ttggggtatt tccggtgtta tgggatccgc aggcgggggt
     4381 attgtcggtg gttaccagtg atcccgacaa tctcgaagtg ctcaaggaag tgcagatatt
     4441 cgcggcggca aaggaggttc gggcttttgt ggcgaggccc gcggccgtgc gggcggcgat
     4501 cgcgaaagct tatggtgggg acattcatgc ctttgccatt ctcgatcgtt ctgcgcacga
     4561 gcagtttcag acgatgttgg atgtgtatga gcgcaacctc gtgagtgaag agacgatggc
     4621 ggttgcgttg gcggatgcca gggagcgaga gcgtgtgctt tcgggcgatg cgttggagca
     4681 atcggcttcg gtggcggtag gcgggctcgg gacggtgacg tcggagccgt atatcgagac
     4741 gctcaacgtt atggtgagtc tgattgagaa cccgcgaccg gatcttcggg gtcattcctc
     4801 gcacgtggcg cgtttgacga aacgcattgc ggagcgcatt gggttggctc cggtggaggt
     4861 tgcttcgatg gtcgtggcgg cgcatttgca cgacctgggc aagatgagcg cctaccatct
     4921 tacggcgctg aacgtgtcgc aatacgaggg gcatcgggta gcggcggaga agtcgtacat
     4981 gacgccgcgg cgcttgctcg agggagtgaa cttgaatccg tcgacgatca aagcgctgga
     5041 gagtatgtat gagcgtttcg atggaactgg gcttccgggg gtgctggggg gtaaggatat
     5101 tccgttgggc gctcgtatcc tcgcgatcac cgatacgtac gcggatttga cacagaatcc
     5161 tcgcaatcct tttcgaaaga cgctgcgtcc catggaagcg agcgaggtgc tcgagcgatt
     5221 caagacaacg gtattcgatc ccaatctggt cgatttgttc aagcatgcgg tgacgggcga
     5281 cgacatgcgg gctcgtttgc ttgccaatcg tcataaggca ttggtgatcg atccggatcc
     5341 ggaggagacg acgctgctcg agcttcgcat gatcgagcaa ggattcgagg tggtgatttg
     5401 tcgcgcttcc gatcaggcgg ccaaagcgtt gcacagtggg gattttgaga ttgtcgtttc
     5461 ggaagtggac ctcaaaccgg aagatgggtt tgctcttttt ggagccatga aagaacaacc
     5521 ctacggccag gatttatcgt gggtatttct tacgcgacgt gcggcgcggg gcgatgtgca
     5581 gaggggattc gacttgggag ccgctgatta tgtcatcaag cctgcgcaag cggacgtgtt
     5641 ggtgaccaag ctcaagcaga tcatcgagcg aaacgcaacc aagacgcgcc acaagggggt
     5701 atcgggttct cttacggaaa tggctcttcc ggacatcgtg caaatcctgt ggcacggcag
     5761 aaaaactggg gctttgcgca ttcgttcggg ttccgaccat ggtgagatcc actttcaaca
     5821 cggagccatc gtcaacgcga tgcatggtcg attgcgcggc gaagatgcct tttatacgat
     5881 gcttcggttg aaatcggggg aattcgcgtt ggaccccaat ttcattcctc aggcaaaggt
     5941 gattcatgat agtcccgagg cgttgttgct cgaagggatg cgtcgtttgg atgagggaca
     6001 aggttgaaat ggcggttcga cggtgtgcgc ttccgagccc aacgtgtggt aacctgatgg
     6061 ggtctcgctt ttttgggtga gtggagtcat ctcgtgagca aggcgcagtc gccacttctc
     6121 ggattcaata acaacgtcaa acaccgtggg cgtatgttcc atattcagac ggaggattcg
     6181 ggaatcaagt atccccacat cattacgcat ttgtttgccg acggcggtcg catcctcaag
     6241 agcaccaaaa cctcttacgc cgagtatatt ggcagcgaga atcttcgcga gacggttcgc
     6301 aacctgatgc aggaacagca taagtccatg ttcattgcgt tgcgtgacgg ccaatttgat
     6361 cacctgatcg acgaaacgat ggacgaaaaa cggggctcgg gcgaacaggg gaaaggtgcc
     6421 gcggagccag agcctagcgt ggcggtaccg gagtcagcgc aaggtcctat ttccagcgcg
     6481 gtgctcgatg tggaagtcct cgagcgagcg gcggcttcgg tgcaaagccg gtctcctctt
     6541 tttggtggcg aggacatgcc tcctcccccc cctaccgtgt tgccaacgca aaagccgagt
     6601 ggttcgtacc gtgcggtggg agtggggccc tccagccaag ccaccgacaa aaacaaaggg
     6661 cattattcgg ctccgcgtcc tgcctccatc ttcggaacag cttcggtgtc cgagtcctcg
     6721 aagacggtgt ttggggaaga tgtcattagc agcaagtcac tcgacgaagt gatcatggac
     6781 tttctttccg aggagttcga cacctcatcg aaccacgaag gggatgaaga ctcggacaaa
     6841 cgtggacgct cgtaaccgag agaaagactt cctcgctcgt gccggggctt tggcgtgggc
     6901 gttgcccacc gatagctggt tgtatgagga cgattgtctt gtcgttgtga gcaaacccgc
     6961 gggcatgtcg gtgtcgggat cggaacacga tctattgtcg cgtgttcgga tcatccttga
     7021 tttccaggcc aagagcaccg aaggtcttgg cgctcccatt catctggaca gagatgtgtc
     7081 tggagtcgtg gcgttggcag cgagcaagca agccaatgca tcgatgagtc ggcaggtaca
     7141 acagcatgct ttgtcgtgga cgttcgtggt tgcggtgtct tgttctttcg acttagcccc
     7201 gagggggcaa agggatgtgg gagtcattcg agatcgaaac ggattgatgc gtgcgagtcg
     7261 gggcaaatcc gataagcgag tccatctcga ctatcaggtg caatcgcgac aaggggaccg
     7321 gtatttgatc gaggtacgat gtgctgatgg accgagggcg atacgagctg tgcttgcatc
     7381 gatgggtatt gctgtcgcgg gggacgtcgt gtttaaaggt cccgaagctt cgcgtttgtt
     7441 gctccatgcc aagcaattga cgttgttgca tccgcggcat gaaggcgtcg tgacctatca
     7501 ggcgcctgag ccatgggcat ttcacgcgtg gatgcatcga cagcaacgag ccgagcaact
     7561 cgacacgtcg agcttggcgc aagcattgaa agaggctgcg tgcaaacgct tctcgttgtg
     7621 tgcgcggggg ctagaggcat ttcggcatgt gcatggggaa gcggaagggc tgcgaggatt
     7681 ggatatcgag tggtacggga accatgcggt ggtgtgggta gaggagcaga cctgcgaccg
     7741 ggccgtggac gggctgctcg cggttcttgg ggagtgggag cccgcgggga tttacttgaa
     7801 aaaacgtccc aagcaggcct cgcgagcgag cgatgggcag ggggcccctt tggtgttttc
     7861 gcatgcggta cgaggacaaa acacaccgga gccgttttcc atcctggagg atggattgga
     7921 gtatctcgtg gacttggggc aagggctcag cacgggattg ttcttggata tgcgtcgaaa
     7981 tcgggcgtgg gttcgacaaa acagccatgg ggcggaggtg ctcaaccttt tttcctatac
     8041 ctgttcgttt acggtagcgg cagccgcggg aggtgccaaa cggacggtga gcgtggatat
     8101 ttcgaagcgt tcgctggagg tgggtgatcg taacctgttg aaaaacggtc ttaaatcacc
     8161 gaatcatgag tttgtctgcg atgatgtccg tcgttgggtc gagcgggcaa atcgtcatcc
     8221 ccatcgtttc gatatgatca tttttgatcc gccttccttt gggacgtcgc gatggggccg
     8281 tttctccgtg atgcgtgatt atgaaagcct cgtgtcgttg acgatgcggt tgttgaggga
     8341 tgggggctgg ttgctggcat gcaccaatca ccgggcggta cggatgggga agctacaggg
     8401 ctggcttcag tcggcggcga gtgcggcggg ttgtcgatgg acggtgttgg agcgagcgtc
     8461 ggcggatctg gattttcccg tgggactgga gggcgagcct catttaaagg catttcggtg
     8521 ccgtgtggca tggaaatgac gacccaacgg tcttggaggg gagatccatg gaacggaagc
     8581 gtaatagaat acggctggtc actgcccgat ctgcccgcaa gcggaacggg tgaggatttg
     8641 atacacttcg gacagttgac gttgttgtgg ttgaggagaa agccatgatt cggacagcac
     8701 ttctggcact tatcgtaggg actcttgcca cgggatgcgt ggtgacgacg ggtggagcgc
     8761 catcgaattc gtctccgacc catagttata aagtgagagg aaaaagtcgg agccacagta
     8821 accacggcgg ttcgcgagcc gcggcaacac cagggacgac gcagcctggg gggcaatctg
     8881 ggggtggtga agcggtggcg acgcctccgg tggtaacgcc tacgacagaa gcgacgccga
     8941 cgacgggcac cacaccgact ccgggcatga cgacaacaca agggctgccg tcggtccatc
     9001 agcaatccaa tcctgcggga acgacgacca cgggagaaga acaaaacggc aatcaaccca
     9061 gaaggcggct tcccaaagcg aacctgccgg tgctgacacc aaccaagtag ccgctgggtg
     9121 ttcgtctatc gaacggaatc cgtgcgaact ggcgtccctc tggagtaggc tcggcatgct
     9181 tcttccaggt tgggaacgat catgtctttt cacgcacgac aactgaccgc cgctgccatc
     9241 gtccttggca tttccattgt tcttgggttc ttgattccca cggctgccac ctatctgatg
     9301 cttgtggggg ccttgggggc tgttgcggtt ttcgcgacct cgctgggtgt caatccgacg
     9361 ggattgcggg agctaccgac agcgatcgcg caattggaac ggggagagcg tccccaactg
     9421 cctgacaacg catcccctta cgtcgtgcag gctttcgaaa aggcttccac gcttgcggat
     9481 agggcgcgtg acgatgggtt gggttcgacc ctcgaggcgt tggggtccgc gcttcgagaa
     9541 gctagcgaag gcaacaaacc ctcgtggccc gaagaccttc ccgtgaacac ccgaccgctg
     9601 tggaagcaac tggcgcaaat cgcatcgtcg atcgagacta gtcagcaatc catcgcttca
     9661 cgcgcgacgg cattgcagcg gtcttcgagc aaggtcgacg aggtggttgc gattctgacg
     9721 aacggattgg ccgatcgtac gaaactcgtg acggaaacat cccgtacagt ggtggatgta
     9781 tcgggccatg cacgggatct tggtcgtcat gtggaagcct tggcggcgac ggcggactca
     9841 tcggcatcga gcattgtgca gatgacgatc acgaacaacg aagttgccac gagcgtgggg
     9901 gatcttgcga tgagtgttcg agacacggcg tcttcgatag agcagatggc gtactccatc
     9961 aaagaggtgg caaaaaatgt agatgcgctt tcgttgacga ccgaggagac gagttcctcg
    10021 atgaatgaga tggatgtttc gatcgatcaa gtgcaatcca atgccaacga gaccgcgcgg
    10081 ttgagtgaag aagtggcgca agatgcggag cgtggggcgg aagcgattct caaaaccata
    10141 ggagagatct atcgcatcaa ggaatccaca ggggaagcgg tgtcggtgat ctcgaatctc
    10201 ggttcgaaaa tcgaagcgat aggacagata ctcaacgtga tcgatgacgt tgcggagcaa
    10261 acgaatctat tggcgttgaa tgcggccatc atcgccgcgc aggcgggtga gcatggtaaa
    10321 ggttttgcgg ttgtggccga tgagatcaag gatcttgcgg agcgcgccgg agcgagtacc
    10381 aaggaaattg ccgagcttac gaagacgatc cagaacgagt ccaaaaatgc tgtcatcgcc
    10441 gtggagcgcg gtgccaagaa cgtggatcgt ggagtcgagg tgtcgaatga agcagagcat
    10501 gcgctcaaaa aaattcttga gagttcccag aagtcgacga acatggttcg agcgattgct
    10561 cgcgcgacgg tagagcaggc gaaggggtct cggcaggtga ccgatgccat tgggcggata
    10621 gcggagacag ttcagcagat tgcggtggcc acggcggagc aagcgcgcgg ttccgaactc
    10681 atcatgcgaa gcgtggaccg gatgcgcgcg attacgcaac aagtcgagca gtcgagccaa
    10741 gatcaagcga aagggggacg ggacgtgaca gcgtcgatgg agagcattac atcgttgctc
    10801 gaacagcttt ctcatgcgca tcgagcgttg tcacgaggtg cggactcgtt ggtggcgtcc
    10861 gccacgcaat gggaacaagc catcgagcaa gaagagtcgg cgttgcatca actcagcggc
    10921 gagacgttac gtttgcgtga tctgcggtga acaaagaggt tttggaccca agggggggga
    10981 cggtcttcgt tgtctgacga accttggcgc ggcgaagggt tcgctgtccg gatgaatggg
    11041 agctaggtcg cgcgtgaggg tagctgatcg caggaagcgt attggcacgc ggtgttctcg
    11101 agattggaag acggatgaaa tcggggggtc attcaccgga ggataggcgt cgttcgattt
    11161 cttcgaggtg tgcgcgcaac tcgggatcgt gatctcgcat ggcttcgact tttcggacgg
    11221 cgctcatgac ggtggtgtga tcgcgattgg cgaaggcgcg tccgatttct ggaaatgagc
    11281 acttgagccg ttggcggcac aggaacatgg cgatgtgccg agcgcgagac acgctgcgat
    11341 gtcgggcctt ggacagcatg tccgaggttt ccacgtgaaa gtggcggcaa acgacttgtt
    11401 ggatgtattc gacggtgggg gtgggttttc tcgtgggttg tgcagaggaa agctcgtgtt
    11461 cggcgaacga caggtcgaga ggacagccga gaagggatga tttggcgatg agccgaatga
    11521 gcataccttc gagttcccgc acgttggact gaacggtttg agcaagcaaa acagctactt
    11581 cttccggcag gtccacattc tcgatctttg ctttttttcg tacgatggcg acacgagttt
    11641 cgaattccgg tgtttgcacg tcagccacca acccccacgt gaaacgcgac accaaccgtt
    11701 gttccatgcg atcgagttgt tgtggatact tgtcggccgc gagcacgatt tgtttgtcgc
    11761 atgcgtgcag gaagttgaac gtgttgaaaa attcctcctg cgtttgcgtt cgtccggcaa
    11821 gggattggac gtcatcgatc aaaagcgcat cgcattggtg gtatcgggct cgaaactcgt
    11881 ccatgcgacg gtccgagatg gagttgatgt actcgctggt gaaagagtcc gccgatccgt
    11941 aggcgatacg agcggaggga cgttcggagc gtatgcgcag ggcaatggcg tgcagcaggt
    12001 gggttttgcc caatccggtg cttccgcaca agaaaagggg attataacgt cgtcctgccc
    12061 ccccggaggc tgtcgaagcg gcggcgaaag caagttggtt gctggggccg acgacgaagt
    12121 tgtcgaaagt gtttttgggg ttgacgccaa gcagggcggc ggtggaggcg ggtcgtttgg
    12181 actcgcggat cacgggctgt ggttcaggct cgacgatctg aatggccctg gggatgacgg
    12241 gatcaggaat gcgtttggag ctgacggggc tatccagatc tgaatcgatg gtccagacga
    12301 catcgagcga ggagcctgtt gtctgttcga tgatgtgaag caaggcaggc tgaaagtgat
    12361 ccctgatcca gtcccttacg aattcgtcgc gtgctcgaag atggagtttt ccgtctacga
    12421 ggccatcgaa ttgcacgccc gcaaaccaag tgtcaaagga cgcgggggag tgctcgcgca
    12481 cttttgcgat ggcatcttcc cacacctggc tgtggattct ttctatcacg ttttccctac
    12541 tagtaacggt aatgagattg atgtctcccg acttgtccac aggttttcca caccctcgaa
    12601 ccaacgaccg aatcgagtta tccacatgct gtggataacc agtggacagt cagaccaaat
    12661 ttcccctcga acaccgcaat gagattcccc gtgtacttgc ggatactatt atcccgtaca
    12721 tgctcgaagt gtcggctcgt caaaaaatgt cagcgacact gattccaatt ttgcttgagt
    12781 cgcttgtcga acccacgcgg tgggaacgtc aacgtgagcg aacccagtgt ttagaacgtc
    12841 acgacgaaga cgtccacgat gatctttcgt ggactgggca acacaatgga atggcgtcga
    12901 atttttttgc cggggcgtcg cgcgagacag atttcgcttg tgatggagag gttcgacgct
    12961 gcgaggcaag tttgtaactc ttggaaggaa caatcgattt gggattgatg cggcctcggt
    13021 gtctctcgtc gctgcaccaa tattgctgat ccatgaagtc aagggaggaa tgatgtcgac
    13081 gtgctcatgg ggggagatta tcgtaattcg tgacaaaata tcggaatacg tgaacttgtt
    13141 gtggtggcgt ttgggtgcag gagtcttgga tgccggggcc ttcggacggt ggttcatctg
    13201 atattttggc caaaatcttg ccgaaagggg gcaaggggat gtagcaccct cgtacaaccc
    13261 caacgtcggt tcatcatgcc ccgaatccgc gtattggcct taacggcaac actttttatt
    13321 tttgcaggta cgatccccaa cgcccatgcg gatcgaagcc gtcttcctcc ggagattggt
    13381 tataactacg gggaggtgga agacgctcga tgggcggcga cgtcgggtgc gttgcgggcg
    13441 gttggcaacg ggatcacggg tatctggggc aatccggcga gtcttgctca ggcgcaagtg
    13501 taccatattg gtgcgattgc gggggtttgg cccgaagcca agcgtcaatc gtacggggtg
    13561 ggggcgatgg attcggtgac ctcccggttg gcggctggac ttggatttgt ttggagcgat
    13621 caagatccgg acggattgaa gcgagagtct cgggaccttc gtcttgcttt ggcctatccg
    13681 ttttcgcctg cggtttcttt tggtctcacg ggtcggtacc tgcacttgaa gcaggacggg
    13741 cttggtcctt tggggcagag ttatgcatcg ggggggctgg cggacgaggc gatgctcgag
    13801 ggtttttcgt ttgatgcggg tcttcggatc gcgccttccg aaaacttttc ctttggcctg
    13861 cttggttcga atctatccaa tccgggacat gggcttcagc cgacgacggc cggagggggc
    13921 ttgggtgtgg ggacgcgtga cttcgtcatc gaggcggatg tgcttgcgga ctttacgacg
    13981 tggcaaaaga cgacgacgcg agcgatggcg ggggccgaat ttttggcggg aaataacttc
    14041 ccccttcgtc ttggctatcg atacgacgcg ggagcgtctt cccatgccat ctccggcggg
    14101 cttggctaca tcgacccggc gttttcggtc gagcttgggg cgcgacgatc ggtagcgggc
    14161 gatgggcata cggtgcttgt tttcacggtt cagtattttg tcgagtcgag tgggatgacg
    14221 cgtgcgccta gcgcggcgtt ttgagggaag gatagcgagc gacgaaccgc ctcgatcgag
    14281 cggttgttgc gtgtcttcga tccttcaatc gtttcttccg cgcgtctggg gacacaacgc
    14341 gacaatggga ttcgaaagac gttctcattc atggcgaacc gtggtagacc gtgtgcgggt
    14401 gtgggtagat tcgaaagaat agcacctgca cctcgggttt ctgcgtatgc ggagtccggc
    14461 ttggagcagg aagccttctc gggctcgcct tatcgaggcc gaggtttggc agcatgctcc
    14521 gttttatggt gaggatgagt ttgcttcgtg cgctcatcca cgtggttagc agcaaagcgg
    14581 caacacggcg ttcggttggc aagcccatgg gcgtttgtgg ttgatgagta aggtttcgcg
    14641 atggaacgac taggcctctc cgcggcaact cgtgcggtca tcctcgttgg ggattacggc
    14701 agtggtaaga cggagattgc ggtgaacctc gcgctgcagc tagcggcgac gcaggcgtat
    14761 cccatcgcga ttgcggattt ggacttggtg aatccgtatt tccgttgtcg cgaggcggtc
    14821 gagcctctcg aacgcgtggg gattcgtgtc gtgattcccc aaggagggca tcgctttgcc
    14881 gaccttccca tcttgctgcc tcaggtcaag ggcttgttac agaacgacga tcagcttgcg
    14941 gtattggatg tcggaggcga tgaggtgggc tcgagggtcc ttgcgggact tgtcaaagcc
    15001 tatcgtcagg gggtccatga attgtggttc gtggtcaatg ccaatcgtcc tttcaacgat
    15061 acggtgcagg gatgtgcggc caccatttcc cggatcgagg gagccagtgg tcttcgggtg
    15121 acggggctgg ttcccaacac gcacttgatg caggagacca cggcggagat ggtaggtgag
    15181 gggattgtgt tggcggatca ggtagccgaa aggttgtcgg ttcccgtccg aatggtagcg
    15241 gtgatggagt cgatggcaga tgccgtgcga gcccatcatc ccgtattgcg tatgcgtagg
    15301 ctcatggttc cgccttggtt gaggcgggac acggtgcagt taggtcgaac gggaccggaa
    15361 gcgttccggc ttcgttcgac gtagggagac gatcgaatgc caagcatcca agtggatcga
    15421 gaccggtgca agggctgtga gctttgcaac gacgcttgcc ctcagggcat ccttgggatg
    15481 tccaagcaga tcaacatcaa gggatatttc tatgcagagg tgattgatcc ggggcgatgt
    15541 atcggttgca tgttatgtgc cgtgatgtgt cccgatctgg ctatcgccgt tcatggcact
    15601 gcggtgcagt atcgattgta cgactacttc cagcctgagc agcggtgagg agaagccatg
    15661 gcgaagaagt tgatgaaggg aaacgaggcg attggcgaag cggcgattca agcgggttgc
    15721 cgcaactact tcgcctaccc gatcacgccg caaagtgagg tggcggaata tctttcgcgt
    15781 cggatgccgg aagtgcaggg ccactttgtg caagcggaga gcgagcttgg ggcggccaac
    15841 atggtgtttg gcgcggcagc cacgggcgaa ctggtcttca cgacctcttc gagcccgggc
    15901 gtcagcttga tgagcgagac catctcgtac attgccggag cgcagctacc cgctgtcttc
    15961 gtcaatatcg tacgcggagg tcctgggctc gggggcattc tcccagcaca atccgactac
    16021 ttccaagcca ccaaaggcgg cggccacggc gattatcgtc tgctcgtcct tgcgcctgcc
    16081 acggtgcaag aagcggttga ccttacgatg ctcgccttcc agcttgcctt caagtaccgc
    16141 aaccccgtca tgattctagg cgatggaatg atcgggcaga tgatggaacc ggtcgaattc
    16201 aaaccaccgc cacctgctgc cgacctgcct tctgtcgaaa catgggccac caccggtgcc
    16261 aaaggacgca agtgcaacat cgcccattcg ttgtttctcg atccagaagc actcgaaaaa
    16321 aataaccacg ttttggctgc caaatacaag gtttggcaaa aagacgaaat ccgcttcgcc
    16381 acgtataatc tcgacaacaa tccacgattg ctcatcgttg cctacggcac gatggcgcgc
    16441 atttgctcta cggccatcga tgaactcaag caaaagggcg tcgaggtggg cttgattcgc
    16501 cccatcaccc tttatccttt ccccgcggcg gccatcctgc agcacgcaaa aaaaatcgga
    16561 aaggttatct cggtggagat gagcatgggg cagatggtgg aagacgttca agcagccgtc
    16621 gggcatgaca ttcacgtagg tttcttcggc cgtgccggag ggatcattcc ctctccggat
    16681 gaggtcgtcc aagccctcga ggccgagctt cgagcatgag acaaggagca cccatgacga
    16741 ccctctttgc ccctcccgta tccatgaacg aacgggccac gcattattgc ccgggctgca
    16801 cccacggcgt gatccatcgt ctcgtaggcg aaacgatcga tgagcttggc attgcggaac
    16861 gcacggttgg catcgctccc gtcggctgct ccgtgttgat ctacaactat tacaatatcg
    16921 actttcaaga agccccccat ggaagagccc ccgcccttgc cacgggagcc aagcgcgcgc
    16981 gtccggacct catggtgttc acctaccaag gcgatggtga cttggcatcg attggtacca
    17041 acgagatcgt tcacgcggcg aaccgaggag aaaaaatcac cacggtgttc gtcaacaacg
    17101 cgatctacgg catgaccggc gggcagatgg ctcccacgag tatgccagga caggtgacga
    17161 caacctctcc tcgaggtcgt gacccagcgc ttgcaggctt tcccatgcga atgtgcgagt
    17221 tgctgtccac cttgcgaacg cctgcctata tcgtgcgcgc ttcggtgcat accccgcagc
    17281 ttgccgtcca aaccaagcaa gccatccgtc gggctttcca gctccaaatg gaaaaccgat
    17341 gcttctcttt cgtcgaagtc ctgtccacat gccccaccaa ctggggcatt cctcccgtca
    17401 aagcccttcg atggctgcaa gacaatatgt tgccgtacta cccgcttggt gtgttcaagg
    17461 acccggacca gcaagaacgc gcggaccgtc aggtccccac gccgtggagg gataacgatg
    17521 caaaatgaag tcatgttcgc cggcttcggc ggtcaaggca tcatgctcat cggtcagatg
    17581 ctggcctatg caggcatggg ggaaggcaaa aacgtcgtgt ggattccttc ctacggcccc
    17641 gaaatgcgtg gaggcacggc ctattgtacc gtcgtggttt ccgataagga tatcggttcg
    17701 cccatcatcg atcggccgga aagcatcgcc gttctgaatc gaccatcact cgataagttc
    17761 cacacccggg tgcgtggcaa cggtttgctc atcatcaaca catcgctcat cgacgccacc
    17821 acggatcgtt cggacatcga tgtgctcaat gttcctgcca atcagattgc gatggaagcg
    17881 ggcacgggca aggcagccaa tatgtgtgtg ttgggcgcgt acgtgggccg caccaaggcc
    17941 gtgacgctcg agtcgttgcg tggcctggtc cgttatcaat tcaaatccaa accacagttc
    18001 atcgacgtga acctgcgtgt tctcgaaaag ggctacgagc tgggggagca gctcgccacc
    18061 aagaataatt agtcacgtct ggagccgctc atgacccaac ccgtcgatat gcaaacgact
    18121 gaagacgtac tggcccgtta tccgagcgat ccgcgccatc tgatttcggt gttgcaggat
    18181 atccaactcg cgtaccgcta ccttcccaaa gaagcgattg cacgcgtctc cgccgccctt
    18241 ggcgttccgt tggcgaaggt gatggccgtg gccacgtttt acaaggcctt ctctttgacc
    18301 cctcggggga aaaagatcct gcgcgtttgc acgggcaccg cctgtcatat ccgaggcgcg
    18361 ccattgcttc tcacggagat cgaaaaacgt ctttccatca agcccggaga caccacccaa
    18421 gaccttgaat tcagcctcga ggccgtcaac tgtgtcggag cttgcgcgct cgcgcccgtc
    18481 gtggtcgtca acgataccgc acacggaaat ctatcggtca ccaaggtgcg ccgtctgctc
    18541 aagcccgcgg aaaagtccac cgagaaggaa gcgaattgat ggacaccaac ctgcgctatc
    18601 aaagtatcga agcgttccgc tctcacgttg cggctctgca tgactcgaag ctagccaacc
    18661 aacgtcgcgt gttggtttgc tgtggaaccg gctgtcttgc gggcggagcg gcacaagtcg
    18721 ccgaggcgat ggcacaacag ctagcggcca tcgaacccga aaaatcagcc caattgcagc
    18781 tcgtcatgaa aaaaaccggc tgccacggct tctgcgaaaa aggccccctc gtcgtccttc
    18841 ttcctcaagg tacgttctac aaaggtgtca aagccaaaga cgttcccgaa atcatcaaag
    18901 aatccatcct ggaagaccgc gtcgtcgaac gtcttttgta cgtcgagtcc agcaccggag
    18961 agcgtgtcga aaaatacggt gacatcgagt tttatgccaa gcagacccgg gtcgcgatgc
    19021 gcaacatcgg tctcatcgac ccggccagcc tcgacgatta tcttctgcgg ggcggatatg
    19081 tcgccttggc gaaagccctg tcttccatga cccccgaaca ggtcatgacg gaagtctccg
    19141 atgcaaaact tcggggtcga ggcggaggag gcttcgacgc gggaagaaag tggcgaagct
    19201 gcaaaaattc ccccgccgac gagcgtttcg tcatttgtaa tggcgatgaa ggtgacccgg
    19261 gggccttcat ggatcgctcc atcatggaag gagatcccca cggcgtgctc gaaggcatga
    19321 tccttggcgg ctatgccttg gagtgcaagc aggggtatat ctacgtgcgc caagagtatc
    19381 cgctagccgt cgaaaacttg cagcttgcga tcgacgaggc acgcgctcgc ggattgctcg
    19441 gagacaatat cctcggaaca ggattctctt tcgacatcga gctttcgcgg ggaggcggtg
    19501 cgttcgtctg tggcgaatcc tccgcgctca tgcgatcggt ggaaggcaag gtcggcgagc
    19561 cccgggccaa gtatgttcac gccacggaca aagggctttg ggacaaacct accgttctga
    19621 acaacgtcga aacatgggtg tgtgtccctc gcatcatcga gcaaggcgcg cagtggtttg
    19681 cctccattgg caccgagcgt tccaagggaa ccaaagcctt tgcgttgacg ggcaaggtga
    19741 agaacacggg gctggtcgaa gtcgagatgg gcacgacgct ccgacagatc attttcggcg
    19801 cgggcggcgg gatgctcaac gatggtccgc gcggcaagtt caaagccgtg cagaccggag
    19861 gcccttccgg tggctgtttg ccagaagaca tgctggacct tgcggtggac ttcgacacgc
    19921 ttacgaaagc cggatcgatg atgggttccg gtggcatgat cgtcatggat cattccacct
    19981 gcatggtcga cgtggctcgt tacttcctat cgtttttggt cgacgagtcc tgtggcaaat
    20041 gcgtgccctg tcgcgaggga ttgtttcaga tgatgcatat cgtgtctcgc atctgtgaag
    20101 ggcaaggcac gatggaagat ctcaataaaa tcgaagattt agccactgct gtcgagcttg
    20161 gttccttatg tgcgttgggc aagagcggac cgaatccggt gcgttcgaca ctgcggtact
    20221 tccgtcagga gtacgaagct cacattcggg acaagaagtg tccggccggg gtatgcaagg
    20281 cgctcattag ctacaccatc gatcatgaca aatgcgatgg ctgcggagct tgtttgcgcg
    20341 cttgttcgtc cttggcgatt gctgggacca ttaagaaggg cgataccttc cagatcgatc
    20401 agctccaatg tgatcgatgc ggggcatgcg agaccgtgtg caaacgcggc gcgatttccg
    20461 cggcttgatc ggcaaggagc aaggagacgt catggcattt ttcatgacca tcaacggtaa
    20521 gcgggtcgaa gcaaacgacg gagagatggt tcttggcgcc gcccgccgtg ctggtattgc
    20581 gattcccacc ctatgtcatc atgaggcagt cgagccgttt ggctcctgtc gtctatgcat
    20641 ggtcgaggtc accaaacccg aatggaaggg atggaagggg ttgatgaccg cctgtctgta
    20701 cccatgcgcg cctgacctca tcgtggagac cgatagcgag cgcgtgcatc aagtgcgtcg
    20761 caacgtgctc gacttgttgc tcgcccgatg cccggatgcg gagttgattc agaagctcgc
    20821 ggccgaatat ggcgtacatc aaacgagctt catgcagcgg gaaaagcccg acaaatgcat
    20881 cctttgcggg ttgtgcgtgc gcatctgtga gacggcggct acttccgcca tctccaccgt
    20941 tcagcgcgga cacgatcgtg agattggaac gccgtggggt gagcctccac ccgattgcat
    21001 cggctgtctg gcttgcgccc acgtgtgccc gacaggtcat atccaatatg aagaaacggg
    21061 gatgacgcgc atgatctggg gacgtcgctt cgagcttcaa cgatgtgaaa aaacgggtaa
    21121 gcccctgccc atcaccaaag agcaagcggc gttcctcgcc aaacggcaaa acatgaaccc
    21181 caactatctc gtgacctccg ccgatgccaa tcgtaagcag actgctgaga cgtttgctcg
    21241 aatcgctcga tggaacaaac tcggtatgca gcaggaggtg aagtaatgcc ccagcgactc
    21301 gtcgtccaac cacaatactg catcggatgc cgagcttgcg agatggcctg cgcattcacc
    21361 catggccaac aaggtcaacc cggcaagagc cgttgtatga cgctcacgat ggccgtcaag
    21421 gatgaatacg ttcccatgct ctgcttgcaa tgcgacaatg ccgcttgcgt gcaggtctgc
    21481 ccggtgtttg ccatcagccg caacgagcaa accggtgtcg ttcacgtcga tatggacaaa
    21541 tgcatgctat gcatggcctg caccgtggct tgtcctttcg gcaacatgca cttcgatgct
    21601 cccaatcgcg ctgtggtcaa atgcgatctt tgcgctggct acggcgacaa cccgcgttgc
    21661 gccatgttct gcccgaccaa gtgcctgtcg gtggaacagc tctgagcttg cctgttgcgc
    21721 tcatcccacc atcttgccca gcagtgagcc cgtgtctttc accaggctca cgctttgttc
    21781 caatcctttg acgatcaagt cgatgtgaag cacgagcttg ggattgagga tctggcttgg
    21841 cgcactcgcc accaaagcca tgcccgtttg cgcggtctca cctgctttgg cggcaagctg
    21901 accgcctagt tccgccatcg cctcccataa cgtggtgagc cttttcgcgc tttcggattc
    21961 accgagctgt gatgtcagcg cctcgatctc gtcctttcgg atcggcttgc gaccgctttg
    22021 cacatccgag ggagcttctt gcccttgggc gttgtacgaa gccaggtata cgtgccttcc
    22081 ggtttccgtg agctggtacg cggccgcgta catttcggcg gaagcacgga aaaaattgtc
    22141 gtactcgggc atgccgatct gcacgtactt gaagtcgtac ttcgcgtttg gattcatgcg
    22201 agccaccttg aggtagtcgt atcgaacgtc gccttgatac ggagccaggg aaggtgaaag
    22261 ctggccgagc tgcacctggc cctggggcgg aacgtccacg agctttttcg caagctcggc
    22321 ggtggtcgac cccacctcct tggcaaaatc cgaaaagtcc gtggccactt ctcccatggc
    22381 ctgctcgaac tcttcccaat tccgagcctc acgaacggtt gtcgtggtct ggccgttgac
    22441 ggtgcgactc gtcacgacgg tggctttcaa cccgatggct tcgcaacccg cggccaacaa
    22501 agaaagacct gccaatttca tccaatgtcg tcgagagatc attggacgca gactatcaca
    22561 cacccacggg cgaacgttgg cggaacgacg ggcttcaaaa caaactgaaa agcatgcgaa
    22621 tggcgaccac atagagaaga accgcaaaga ctttttttag tttgtcgacg ggtagggcgt
    22681 gggccagacg tactccgagt ggcgcggtga gcacactgac gagcacgatg ccggccaagg
    22741 caggaaggta aacgaagccg atggaccaat ccggacggcc ttcgatcccg cttccgttgt
    22801 agacgtagcc cacagcacca
//
LOCUS       DAOSOR010000020        22579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_2298_length_22579_cov_4.522554, whole genome shotgun sequence.
ACCESSION   DAOSOR010000020 DAOSOR010000000
VERSION     DAOSOR010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 22579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22579
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2298_length_22579_cov_4.522554"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /gene="nadA"
                     /locus_tag="PK710_02020"
     CDS             complement(<1..785)
                     /gene="nadA"
                     /locus_tag="PK710_02020"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOT08511.1"
                     /translation="MPTEPELFRARCGFEPLAIGYGGLYKTGMNYTRHSEGIDQDIDP
                     SLDLEQEIARLKKEKNAIILAHFYQVPEIQDIADFLGDSLQLAQQAKKTTADVICFAG
                     VHFMAETAKILNPERIVVVPDLEAGCSLADGCPVDAFRAWRAKYPDAVAVSYINCSAA
                     VKAESDWICTSANAVRVVQAIPPEKQVLFAPDRNLGRHVMKHAGREMQLWPSTCIVHE
                     TFSERKIIDLKERHKDALFIAHPECEPAVLRLADYVGSTTALLR"
     gene            947..1837
                     /locus_tag="PK710_02025"
     CDS             947..1837
                     /locus_tag="PK710_02025"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT08512.1"
                     /translation="MKTEPPRRNTRLGPSTKTTSWLVAVAGALLLLVLFVGHRHAAPP
                     SRCPDGLVAHGGRCCAPGQLAKSGKCIGKPKGCPAGFSLREQGCVARPSLVPIPSATI
                     RIGPGDWEAQGQVVARDVVVASPFRIDALEVTFDRYAACVAAHACEPTAHDGEPGQPV
                     AGVTLSQAIAFCHWAGGTLPSEDQWIVASAGPRARRYPWGDSGAVCRRANWGTLAGPC
                     AHGARGPDWAGAIELDRTPEGVLGLSGGVSEWVRATEPGVVRGGSYRSQLASQLRTWW
                     RETRDPERGYEDVGFRCVYP"
     gene            1929..3161
                     /locus_tag="PK710_02030"
     CDS             1929..3161
                     /locus_tag="PK710_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HOT08513.1"
                     /translation="MTAAILSIGTEITRGEIVNTNATWLADRLTQVGMEVAEVVSVDD
                     DCPRIIETLRRLAATHDVIVSTGGLGPTTDDLTALCAARAAQVSLVRDPASVEEIRDR
                     FRIFRRRMGPSNIKQADIPSGAVVMTNAVGTAPGFWMCLGRAKVFFFPGVPREMVHLW
                     EKHAQAVIGSYAKEPTAQIRLHTFGEGESAVAERLADVETLFSGVIVGYRASFPVIEV
                     KLLARGAGAQALVEAAADFVRGRLGSLIYGQGEQSFVSVVAQAVRNSGLRLALAESCT
                     GGLMAQLLTSEPASDYLAGGLVTYANDAKVKLLGVPESLLEEHGAVSEPVAKAMAIGA
                     ARAMGAALAVATTGIAGPTGGTEDKPVGLVYYAVAYPGGVQVRHQVFFGDRSRVQRIA
                     AFSALQLLRTCIASPTGC"
     gene            complement(3172..4521)
                     /locus_tag="PK710_02035"
     CDS             complement(3172..4521)
                     /locus_tag="PK710_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOT08514.1"
                     /translation="MQPGPPELPGGALAESPIDRALALSLAGERDAALRWAAAVVQHD
                     PGMPSGLLLCGRLLAETNRLEIAREALEICLYSSIDAGNLPLAVAACSDLRNLGTDPD
                     PLFDAIALAFGLGSSRLQAAAAPPILPQTASVQPLPSVLTGMALVNRTADILRTARKA
                     RKDLEHTRHAAPPISPVALFSDLDPPSLRALIEQFEVMSVPKDALIIQEGDEGAEAYI
                     VARGELEAIRTVDDQTTVLARLTAGSLFGEMALLSRAPRAASVIACRPSILLVARKDA
                     LDQVAVGFPSVGTVLASHCQRRMVQNLIRTSAILRAVGPDERPALIERFVTRTFEPGE
                     ILIEQGETAAGLHLIASGEVSVSREENEETLVLASLGPGDIVGEVSLVLRKPSTARVT
                     AKHATLCLHLPTEDFLELIREHPAILAELYDLAVRRDEETSTIVAQEVFDVDDALLI"
     gene            4715..5296
                     /locus_tag="PK710_02040"
     CDS             4715..5296
                     /locus_tag="PK710_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08515.1"
                     /translation="MTRRQLLGMLGAVGAVVIGIPVGWSALAAVRFPSDRTPEGAYLR
                     IVLALNKGNVRDAFPYLETEAQHALCTIHAYRRKALEIIRKSFREPERSQWENAYREQ
                     GDAKDPPELWARLAEKNGWVARLRKDLSGIASIERIGKRATIETTGATRYPFREADNG
                     IWGLTLFTAELVAHKERVSRDLQIIEKTASDYG"
     gene            complement(5287..5415)
                     /locus_tag="PK710_02045"
     CDS             complement(5287..5415)
                     /locus_tag="PK710_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08516.1"
                     /translation="MTQHLGSRFLFRVDGMETVGLRWEDRTLALGSDSHPPWFGYP"
     gene            5414..6373
                     /locus_tag="PK710_02050"
     CDS             5414..6373
                     /locus_tag="PK710_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08517.1"
                     /translation="MLIHLVFKIGRSVLWVSLIALLLGAAGALVRVLPWIVADDVPWS
                     VSFTFFRTLVLASTEVALFIALPLGCALEVVRWTHDGVALTLRSLGVGPYQQAMQAMP
                     VALIVGLFTATVSYPSALIASYPGVVSNSLLDTAASQACRHERAVRVPALPVAWLCSQ
                     HQKRLVGWYPAHHPPMGVLTASHARFTPAMDRLELEDVVWVSAPSSTLRARNVVITGV
                     VPGVVAARIPPALRAFAFGSLACLAALGTSFALLRWPLASRPRALVVGTSATLGMLLG
                     NQALTQWSWLGWLVVALLTLCGPIAFAWATRLTWLPITSPGGT"
     gene            6603..6800
                     /gene="tatA"
                     /locus_tag="PK710_02055"
     CDS             6603..6800
                     /gene="tatA"
                     /locus_tag="PK710_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOT08518.1"
                     /translation="MGNIGPGQLLLIALIVLLIFGAGRIAEIGKGLGAGIRNFKKGLH
                     EDDEDEKKQLTEGSKDNDKNA"
     gene            complement(6928..8334)
                     /locus_tag="PK710_02060"
     CDS             complement(6928..8334)
                     /locus_tag="PK710_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08519.1"
                     /translation="MIKIFSWAIAFAPFVFVACGPGGESSVTNAQAGSGGGTSQDAAA
                     EAGACQPATCLSAHAECGVIADGCGKTIACGGCEHGQVCGGGGTNRCGIHACTPKTCI
                     QLGAACGIISDGCGNVLDCGSCPMGMTCGNTQIQFQCVAVDGGTAGTGGTAGSGGSGA
                     NPTCTPGEACDDGDACTVGDLCNHAGQCEGGTPVSCTSPPNGCFVSSGICDSLSGLCS
                     YPYKVSGTPCDDEGNACTIDQCDGAGQCVHIAKSAGTPCPGGTCVNGVCNASCPGGVN
                     VTWSQSQSAWNGNPKTAGNYTCSATLPSGSHPHGATVPVVPNQPKKRMGNAVATCESG
                     AWKVSGSCDGNVIDTAMKTECAPSSPPSRKLIASWYVADLVRCADSAGLDYWVSAYDD
                     PASQTCTAASNYQGMGSKDACWRATFRDSANAAGCYDQTQATGHICDSAASNLCEPGS
                     YYPWTSILSAGTTCKVGP"
     gene            8537..9085
                     /locus_tag="PK710_02065"
     CDS             8537..9085
                     /locus_tag="PK710_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08520.1"
                     /translation="MRIQLLLAAALGLSVLSLPHDVCADESAEAPAPSLEGTYAPQNP
                     KAAQAVIDNAIESVVQEMNFIKRPFARSRLRDTNPLLTQVRFQRQDDLLIYTYNAGNV
                     IRLVPNGPAIRWKSPADGNTYETKATLVGRRFTLFFKGEEGSKKEVFELGADGTTLTV
                     HSVITSPQLPKALEIRYTLKRK"
     gene            complement(9262..10203)
                     /locus_tag="PK710_02070"
     CDS             complement(9262..10203)
                     /locus_tag="PK710_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOT08521.1"
                     /translation="MASLRLANVVYAGEPLIAIEHLGELFSVRKLEECFALEWSPTHF
                     TDRANEYRQRVFSLGMAGLIELAESLGDIPGPRQAVLDQTLCLYRPPTVDSPALAEFS
                     VLAEDEVPRFRWGNSRCLRGHEAPLPISDDEPSPQLSVQVGAILIDELDHATEEQSDR
                     AIGGFTLLSLWSYPSRNRVSPGWGSFRLGQLGPVLVANQAQDPSRWTVSIRVNGQTVV
                     QAPSKPWRTSFPQMVALASEAAALAPGDIIASGPLARTSSDGRRSLRAGDRIEAEVAG
                     LGVLSGVIVPSHHKSRFLSRTMNDGDKAKTLPPRGAS"
     gene            10514..12175
                     /locus_tag="PK710_02075"
     CDS             10514..12175
                     /locus_tag="PK710_02075"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOT08522.1"
                     /translation="MSNRTKFIFLTGGVVSAIGKGLTGASIGALMEARGLRVTNMKLD
                     PYLNVDPGTMSPIQHGEVFVTDDGTETDLDLGHYERFTQTVASRRSTCSSGSIYEAVL
                     ARERQGGYLGATVQVIPHVTDEIKNRIRNAARGYDIAICEIGGTIGDIESLPFLEAIR
                     QMRVELGKENTISIHLTLVPYIAAAGELKTKPTQHSVKEMREIGIQPDILVCRTDRPL
                     DKSIKGKIALFSNVDADCVITAMDVAFIYELPLLLHHEGIDVRITEQLNIWARSPDLE
                     AWKRVCERYKNPTQGTVRIGVVGKYVDLRDSYKSLSEALVHAGLVNDVRVDLEYIASE
                     TIDSQNVQQILAGLDGVLVPGGFGERGVRGKIEAIRHAREMRIPFFGICLGMQLAVIE
                     FARNQLKLEDADSTEFNKHTRHAVIDLMPDQRNVTDKGATMRLGAYPCVLKKGTMAAQ
                     IYGATEISERHRHRYEFANAYREPMEAAGMVLSGFSPDSRLVEVIELPDHPFFIACQF
                     HPEFRSKVMKAHPLFVNFLQAAMKARTQNDSTQSAAPSNPTNEQA"
     gene            12219..12767
                     /locus_tag="PK710_02080"
     CDS             12219..12767
                     /locus_tag="PK710_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HOT08523.1"
                     /translation="MTELEHECLDRFPHWLDCLHEDVVALASLLTAESIPEGAQRHIA
                     GALNYLTKSLDLIPDGVEDLGYLDDAFVIRVAASLAVAETPSVREVDIRGLLVRLAQE
                     AKLIEQWMGNDFPRLVKVVRELGKGASRGRSVDDIITDPAIRAEAIREVQSWSKTYQK
                     PSFHKDPQTLIKLRSFLSSRLP"
     gene            complement(12777..14450)
                     /locus_tag="PK710_02085"
     CDS             complement(12777..14450)
                     /locus_tag="PK710_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT08524.1"
                     /translation="MTAKSSDKNLGKVIAGRYRLETRIGEGGMGVVYRARHVLIDRVV
                     ALKLIRPDLRGETHLRAWMLREARAANRVDHAHIVDIHDIGETDEGELYLVMEYLVGI
                     SLSSEIARGPFPVVRAVDILEQMCAALARAHDLGVVHRDLKSDNIMLTVKGGRKDSVK
                     ILDFGLAGLARDPRLAPKGAVFGTPEYMSPEQARGEEAGPKSDLYALGVLFFEMSIGQ
                     LPFKSPDRDTLLEMQRNAPAPRPSKLRPDLPPAAESIMLRLLEKDPRKRFLDAHHLQE
                     QLKALQRSLPSRNWDVGGQGETPAPPPPPPPQSEGVTEWANRAALFSRMVARAYHQGN
                     APRELEEGVAKAWDLAARASRLEGEVATHTRKLEALEKRGRGLRAEIGRKVEELAHEE
                     SRALREVAKHQEELERMATEMRTAEREAAAAKHAADAAEKTRTFNRAIFERAGAAIAR
                     ATLLHEQVEKLQQLRASREVVARDLRRQIEELRNQLVRYAEALEEDLAAGREKVAVRT
                     REGIAFERSFSEVCGLLIAHLKNKPEVRDLMNELMPPSADRPTRSQDVR"
     gene            complement(14550..15209)
                     /locus_tag="PK710_02090"
     CDS             complement(14550..15209)
                     /locus_tag="PK710_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08525.1"
                     /translation="MHNPTLRTTVAFAALAIATVSRGAMAEEAGIDATGKGITGGALL
                     GGELVMAVEAAVGVQNTWAYVGGGVAGAAVGGVGGYFVEQGSDPQPAYYMLAGGLALL
                     IPTTVAILQATSYKAPDHYLADEPGADTGPEEIPTGPQTPGTEVTVTAPEAKASPKKS
                     TPVASRPYVPTSLVDIHDGAFQVGVPAVQVLPVYSALETKKYGVEPKTELRLPVFHAT
                     F"
     gene            15522..16754
                     /locus_tag="PK710_02095"
     CDS             15522..16754
                     /locus_tag="PK710_02095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT08526.1"
                     /translation="MFDVLLTEQERAVRDEARAFVRDQVDPQLLRDMDADKVEYPTEF
                     VKNLAKANLLGLRFDPAYGGRALSWAAEFAVLEEIGVLGTALGCAYSMPSIVGEALSR
                     FGTEEQKQKYLGPILRAELISAEGLTEPRGGSDFFGASTIARRDGDDYLLTGQKRFVV
                     GAVGADIFLIYARTGDANAKPHKAISAFLVERSMGVEVKHVYGLMGTRGGGTGRIVLR
                     DVRVPARNLIGPLHAGYDVFNRMMIPERLTSAGGAIGMGRSVLEVATRYAHKRKAFGR
                     FIRDFQGVSFRIAESVTRLDTARALCFAAGKTVESGVDARRIVSEAKKVATEAAWATV
                     NDAMQIMGGIGYTHVFPVERMLRDVRLALIWTGTNEIMNLLVQHEYAKELLGRSFKVR
                     DVQQDAVAPEHVDEIVYE"
     gene            complement(16795..17334)
                     /locus_tag="PK710_02100"
     CDS             complement(16795..17334)
                     /locus_tag="PK710_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08527.1"
                     /translation="MATFSCAENTPAPVTPKTAEAPPPLPEPSALPKGHVWRYQVMEV
                     LSPGLGAFLQRLDVKEKMVDGRFYGFQIVALTGDPDFWQGTDLRAGDVITSVNASPIG
                     HYDEAFRVWQSLATAPQIVVAYERGTEQREIRIIIHEDDDSASKAPPADTGKSSAQPT
                     PSASVAPAVSSAPSPSKQP"
     gene            complement(17453..18112)
                     /gene="tmk"
                     /locus_tag="PK710_02105"
     CDS             complement(17453..18112)
                     /gene="tmk"
                     /locus_tag="PK710_02105"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOT08528.1"
                     /translation="MIEGQFIVLEGIDGSGRSTQAALLGQWYRNQGLPVLVTREPSEG
                     PIGAMIRQVLTHRLVVSGIAGPRAPTWATMALLFAADRLDHLDSLIVRNLMDGVTVIS
                     DRFDLSSIAYQSITSAAEVDPSVVEWVQTINGRARRPDLTIVLDVRPEIAARRREQRS
                     YSTELYEDSVLQEALAKAYETAEAWVPRDLVVHIDGNPDEQTVHERIVAEVRRFRGDK
                     R"
     gene            complement(18109..19002)
                     /gene="panC"
                     /locus_tag="PK710_02110"
     CDS             complement(18109..19002)
                     /gene="panC"
                     /locus_tag="PK710_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOT08529.1"
                     /translation="MISGSLPIYTDLSTYRQACDRARAQEKRVGLVPTMGALHDGHFA
                     LIDEARRHADFVVVSIFVNPTQFGPSEDLDKYPRTWESDVEGCISHGASCVFHPSVPT
                     MYPAGEQTRVRVGAIAEPLCGAFRAGHFEGVATIVTKLFAATGPCVAVFGRKDYQQIK
                     VIERLTTDLLLPVTIVGHPTVREADGLAMSSRNRYLNEEQRQRAAAIPRGLNRAVRAY
                     ERGERKVASLRHEVVSCVAPVADSIDYVEIADADTLEPMDEQRHIAHRALIALAIRVG
                     PARLIDNIVLGEDPPIKGQAE"
     gene            complement(19054..19488)
                     /locus_tag="PK710_02115"
     CDS             complement(19054..19488)
                     /locus_tag="PK710_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08530.1"
                     /translation="MRKFFVFLLSLTFMACAQAPEEETVAGEALASLDATQSPEATIL
                     QIANEFSRDDLLAHDVEPAVADAIVLARQHGPFTSPAQVEQAMTMSLYSTNPLCSYYQ
                     GMAQYYTIRALQCAYSICAPMSPWYAFMAQYYQGLWEANCSS"
     gene            complement(19860..21509)
                     /locus_tag="PK710_02120"
     CDS             complement(19860..21509)
                     /locus_tag="PK710_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT08531.1"
                     /translation="MGYPLQIATRYLGAKSKGAFVSVGTAFAILGVTLGVAALLTVMS
                     VTGGFQSEFRKKVLGVNAHVLVLKYSSDFREYREIMAMMREVPGIIGVAPFTINPMMV
                     SHKEKTATGVLVKGVDPELMPTVLDLPSHIKEGGSLHGLRKPGATPPERKTRGGITPA
                     PKPLPLGSKTAEPKDGGTERDDAGRNITLLRAIEDSIREDERNAKPKPPEGPPDGIDE
                     ILEVEHQPPPLDPSSDTLDAGVAADVMVGSVVPEGGYKSQLPDPEDDLPEDVAPDPCN
                     SPEAVAQLPGIVVGNTLAQNLSVQVGDCLQVTSPTIGFSYSGGVIRPPVAKQFKVIAI
                     FEAGFDQYDSKLVYVDLYEAQRFQDYGDSVTGVEAKVRDIDEAGKIELDVASRLSSNL
                     YSTMDWEDLNHGLFTALRIQKIGMSAVLALIIFVAAFTVIATLIMLVLEKKKEIAVLK
                     AMGASDVAILRIFVYQGGIIGVMGTALGLGLGLAVCKGLLVYGFPLDPKVYFISTLPV
                     EVRPTEFIITGAIALTLCLIATVIPSLYAARLRPADGVRPE"
     gene            21665..22264
                     /gene="wrbA"
                     /locus_tag="PK710_02125"
     CDS             21665..22264
                     /gene="wrbA"
                     /locus_tag="PK710_02125"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF002999.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HOT08532.1"
                     /translation="MTKVAIIYYSSTGTNEAMARAVAEGAKEAGAEVRVRLVAETAPD
                     AAISRNPAWRSFVDAHQNDPRASLDDLEWADAIIFGTPTRFGNIASQLKAFIDTTAGL
                     WLQGKLSNKVYAGFTSAQNAHGGQESTLLALFNTVYHYGGFIVPPGYTDPLVFASGGN
                     PYGPSVTVGSGGPVGQADLAHGRYLGKRVAEVASKLVAC"
     gene            22388..>22579
                     /locus_tag="PK710_02130"
     CDS             22388..>22579
                     /locus_tag="PK710_02130"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOT08533.1"
                     /translation="MSLKPGVVTGDEYKKLVDAAKAGGYALPAVNVTSTNTLNAVLEA
                     AAKAKSDVIIQLSNSGAQFY"
BASE COUNT         4670 a         6656 c         6535 g         4718 t
ORIGIN      
        1 cgaagcaacg ccgtggtgga acctacataa tccgcaaggc gaagtaccgc gggctcacac
       61 tcgggatgcg caatgaatag cgcatctttg tggcgctcct tgaggtcaat gatttttcgc
      121 tcgctgaagg tttcgtggac gatgcaggtg cttggccata gctgcatctc acggccagcg
      181 tgtttcatga catggcggcc caggttgcgg tcaggcgcaa aaagcacctg tttttccggt
      241 ggaattgctt gaacgacccg tacggcgttg gccgatgtgc agatccaatc gctctcggcc
      301 ttcaccgccg ccgagcaatt gatataactc acggcgaccg catcgggata cttcgcacgc
      361 cacgcccgaa acgcatcgac gggacaccca tctgccaggg aacatccggc ctcgaggtcc
      421 ggcacgacca cgatccgttc cggattcaag atcttcgccg tctccgccat gaaatgaaca
      481 cccgcgaaac aaatgacatc cgcggtcgtt tttttggcct gctgcgcgag ttgcaaggaa
      541 tcgcctagga agtccgcgat gtcttgaatc tcgggaacct gatagaaatg agccaaaata
      601 atcgcgtttt tctctttctt cagccgtgca atctcctgtt cgagatccaa actcgggtcg
      661 atatcctggt cgatgccctc ggaatgccgt gtgtaattca tgcccgtctt gtaaagcccc
      721 ccgtagccta tggccagcgg ttcgaacccg caacgggctc gaaaaagctc cggttccgtc
      781 ggcatttcct tcctatttca gggctttatg ccccgctcgg ccgttcgatt ccttcccgtg
      841 gccttggatg tcgaacaagc gatgctggcc ttttgggtcg agcctcgcca caattcaacc
      901 ccatgcccta tcgccgctcg gctttcgatt cccccaccca cgccgcatga agaccgagcc
      961 ccctcgccga aacacccggc tcggcccctc gaccaaaacc acttcatggt tggttgccgt
     1021 cgcaggagcc ttgctgctgc tcgtgctttt cgtgggtcat cgtcatgccg cgcctcccag
     1081 ccgatgtccc gatggtctcg tcgcgcacgg cggtcggtgt tgtgcccccg ggcaattggc
     1141 caagtccggg aaatgtattg gaaaaccaaa gggttgcccc gcgggtttct cccttcggga
     1201 gcaaggctgc gtcgcccgtc cctccttggt gcccatccca agcgcaacga ttcgcatcgg
     1261 tcccggcgat tgggaagcac aaggccaggt cgtcgccaga gacgtggtcg tcgcttcccc
     1321 gtttcgaatc gatgccctcg aggtaacctt cgaccgctat gctgcttgcg tggccgccca
     1381 tgcttgcgaa cccacggcac acgatggcga acctgggcaa cccgtcgcgg gcgtcacgct
     1441 gtcacaggcc atcgcttttt gccattgggc aggtggcacg ttgccttccg aggatcaatg
     1501 gattgtggca agcgcaggcc cacgcgctcg tcgctatccc tggggggata gtggagccgt
     1561 atgtcgtcgc gccaactggg gaacgttggc agggccctgc gctcatgggg ctcgcggtcc
     1621 cgattgggcc ggcgccatcg aattggatcg cacccccgag ggcgttttgg gattgagcgg
     1681 tggcgtttcc gaatgggtgc gagccaccga accgggcgtg gtgcggggag ggtcgtatcg
     1741 aagccaactc gcctcccaac tcagaacttg gtggcgcgaa acaagggatc ccgaacgtgg
     1801 atacgaagac gttggattcc gatgcgttta tccgtgagcc ttgcactctt cgccaactac
     1861 ccaaccatca caggcatggc cgatagaccc gttggccatg gccttggttc gaactacagt
     1921 caccaaccat gacagccgcc atcctcagta tcggaaccga aatcacacgg ggcgagatcg
     1981 tcaataccaa tgccacctgg cttgccgacc ggctcacgca agtgggaatg gaagtcgcgg
     2041 aggtggtgag cgttgacgat gattgcccac ggatcatcga aaccttgcgt cgcctcgccg
     2101 cgacccatga cgtgatcgta tccaccggag gactggggcc aacgaccgat gatctgacgg
     2161 ctctttgtgc ggcgcgagca gcgcaggttt cgctggtgcg agacccggcc tcggtagagg
     2221 aaatacgtga tagatttcgc atatttagac gaaggatggg gccttcgaat atcaagcaag
     2281 cggatatccc ttccggggcg gtggtgatga cgaatgcggt gggaacggcg cccggatttt
     2341 ggatgtgcct cgggagggca aaagtgtttt tctttcccgg cgttccgcgg gagatggtcc
     2401 acttatggga aaaacatgcg caagcggtga ttggctctta tgccaaagaa ccgaccgcgc
     2461 aaattcgtct tcatacgttt ggagaagggg aatccgcggt ggcggagcgt cttgcggatg
     2521 tcgagacgct cttttcgggt gtcattgtgg gatatcgggc ctcgtttccg gtgattgaag
     2581 tcaagctgtt ggccagggga gcgggagcgc aagcgttggt ggaggcggca gccgacttcg
     2641 ttcgtgggcg tcttgggtct ttgatctacg ggcaaggcga acagagtttt gtcagtgtgg
     2701 tagcgcaggc tgttcgaaac agcgggcttc gattggctct tgcggagtct tgtacgggtg
     2761 ggttgatggc ccagcttctg acgagtgagc cggcgagcga ttatttggcc ggaggtttgg
     2821 tgacgtatgc caacgatgcc aaagtgaagc tgttgggagt acccgaatcg ttgctcgagg
     2881 agcatggggc ggtgagtgag cctgtggcca aagcgatggc gataggagcc gcccgcgcga
     2941 tgggtgcagc tttggctgtt gctaccacgg gtattgcggg acctacggga gggaccgaag
     3001 acaaacctgt tgggctcgtg tactacgccg tggcgtatcc aggcggcgtg caggtacgac
     3061 atcaggtatt ttttggtgac cgttcccgcg tgcaacgaat cgcggccttt tccgccctgc
     3121 agctactgcg gacctgcatc gcgtccccca cgggatgttg aaaccgcggc attagatcaa
     3181 gagcgcatcg tccacatcga agacttcttg cgccacgatg gtggatgtct cttcgtcgcg
     3241 tcgaaccgca aggtcatata gttccgccag gatcgcggga tgttcgcgga tcaattcgag
     3301 aaagtcttcg gtgggcaaat gcaggcaaag ggtggcgtgt ttggcagtga cacgggcggt
     3361 ggagggtttg cgaagcacga gggaaacctc tcccacgata tctccgggtc cgagggaggc
     3421 gagcacgagc gtttcctcgt tttcttccct cgagacggaa acctctcccg aagcgatgag
     3481 atgcaaacct gctgctgttt ctccttgctc gatgagaatc tctccaggtt cgaacgtacg
     3541 ggtgacgaac cgctcgatga gagcgggccg ctcatcggga cccaccgcgc gcaggatcgc
     3601 gctggttcga atgagatttt gaaccatgcg acgttggcag tgggaggcga ggaccgttcc
     3661 cacggaaggg aagccgacag cgacttggtc gagggcgtct tttcgggcga ccagaagaat
     3721 gcttggacgg caggcgatca cgcttgcggc acggggagcg cgagatagca atgccatttc
     3781 tccaaacagg gagcctgcgg tgagacgggc aaggacggtg gtttgatcgt cgacggtccg
     3841 aatggcttcg agttctcccc gtgcgacgat ataggcttcg gcaccttcgt cgccttcttg
     3901 gatgatcaag gcatctttag ggacggacat gacctcgaac tgttcgatga gggcacgcaa
     3961 cgagggcgga tcgaggtcgg agaaaagagc gacgggtgag atggggggtg cggcgtgtcg
     4021 tgtatgttcc aagtcttttc gcgctttgcg ggcggtacgt aggatatcgg cggtgcgatt
     4081 gaccagggcc atgcctgtca atacggaggg caggggttga acggaggccg tttgcggtag
     4141 gataggtgga gcggcagcgg cttggaggcg cgacgatccc aagccgaagg caagggcgat
     4201 ggcatcgaag agggggtctg ggtcggtccc caggtttcgt aagtcgctgc aggcggcgac
     4261 agcgaggggt aagttgccgg cgtcgatgga ggaataaaga cagatttcca gggcttctct
     4321 ggcgatttcg agtcggttgg tttccgcgag caaacgtccg cacagcagca gtccggaagg
     4381 cattccagga tcgtgttgga cgacagcggc cgcccagcgc aaagctgcgt cgcgttcgcc
     4441 agcgagggac agagccaacg cgcgatcgat gggcgattcg gcgagggctc ccccgggcag
     4501 ttctggagga ccaggttgca tgggtaggct tgtatagcat gccatcgggc cggtacaggg
     4561 ggggaaagaa gtcgaattat caccaggccg tgggtcggct gtttcggcgc tgctcccaca
     4621 accataaagg cttgcaagtt cgtttttgcc caccattccc ttagaaagac ggaaacccta
     4681 ttcagtgaca agtccaaggg ctaggctcgc caccatgacg agaaggcaac ttcttggaat
     4741 gctgggtgcc gtcggagcgg tggtgatcgg cattcccgtc ggctggtccg cgctcgccgc
     4801 cgtgcgtttt cccagcgatc gaactccaga aggagcgtat ttgcgcatcg ttctggccct
     4861 gaacaaggga aacgtccgcg atgcgttccc ctatctcgaa accgaagccc aacacgccct
     4921 ttgcaccatc cacgcgtatc gtaggaaagc ccttgaaatt attaggaaat cctttcgtga
     4981 accggaacgg agccagtggg aaaatgctta tcgcgaacag ggagatgcca aggacccacc
     5041 cgagctatgg gcgcggctcg ccgaaaaaaa tggatgggtg gcccgtcttc gcaaagacct
     5101 ttccggaatt gcatccatcg aacgaatcgg gaagcgagcc accatcgaaa ccacgggcgc
     5161 aacacgttat ccatttcggg aggccgacaa tggcatctgg ggcctaacac tattcaccgc
     5221 cgagcttgtg gcccacaaag agcgagtctc tcgagatcta caaatcattg aaaaaacagc
     5281 atccgattac ggatagccga accacggcgg atgcgaatca ctccccaagg ccaacgttcg
     5341 gtcctcccat cgaaggccaa ccgtctccat gccgtcgaca cgaaacaaaa accgcgatcc
     5401 aaggtgctga gtcatgctga ttcaccttgt tttcaagatc ggacgaagtg tgctgtgggt
     5461 cagcctgatc gccttgttgc tgggagccgc gggagccctg gtgcgcgtgt tgccctggat
     5521 tgtggccgat gatgtaccat ggtccgtgtc gttcacattt tttcgaaccc ttgtgctcgc
     5581 atcgacggaa gtcgccctgt tcatcgcatt gccccttgga tgcgcgctcg aagtcgttcg
     5641 ctggacgcac gatggggttg cgctgaccct gcgctccctc ggggttggcc cttatcaaca
     5701 agccatgcaa gccatgcccg tggcgctgat cgtcgggctt ttcaccgcca ccgtatcgta
     5761 tccttctgcc cttatcgcct cgtacccagg cgtcgtttcc aattccttgc tcgatacggc
     5821 agcttcccaa gcctgccgcc atgaacgcgc tgtgcgtgtt cccgcccttc cggtggcctg
     5881 gctttgttct cagcaccaaa agcgcctcgt cggctggtat cctgcccatc acccacccat
     5941 gggggtattg accgcaagtc acgctcggtt cacaccggcc atggatcggc tcgaactcga
     6001 ggatgtcgtg tgggtttccg ctccttcctc gacgcttcgt gcccgaaacg tggtgattac
     6061 gggggttgtc cccggggtcg tagctgctcg gataccaccc gcgttgcgag cgttcgcgtt
     6121 tggaagcctt gcttgcctgg ccgcgttggg cacctcgttt gccctgctgc gttggcccct
     6181 tgcttcccgc ccccgggcct tggtggttgg caccagcgcg accctcggca tgcttcttgg
     6241 caaccaagcg ctcacccaat ggtcttggct gggatggctc gtcgttgccc tgctgaccct
     6301 ctgcggaccc attgcattcg catgggctac ccgtttgacc tggttgccca taaccagtcc
     6361 gggtggtacg tagcttcagc caactcgtcg gccattgaat acgcgcagat gtcagccctc
     6421 ggatatgctc gaaacccaac cttcgctcgg cttgccaccg cggttgtcga tgcataccgt
     6481 ctattggacc ccagacagat accgggccgt aaacggcatg agatcggagg cggattcgag
     6541 cgcagctttc gcgacgggtt ttccgaggac tgccctacat tggcagcatt ggagtcaaat
     6601 ccatggggaa tataggtccg ggacaactat tgctcatcgc tctcatcgtc ctgcttattt
     6661 tcggagctgg gaggattgcc gagatcggca aggggcttgg ggcgggcatc agaaacttca
     6721 aaaaaggtct tcacgaagac gatgaagacg agaaaaagca gcttacggaa ggaagcaagg
     6781 ataacgataa aaacgcttga aattcatgat gttactcgat tcatggaccg gtatggggcc
     6841 gaggatgtcg gcggttggcc ggtcgaaaaa acaacggaga ctcctggttg gcggtcatcg
     6901 tgaccgctag gtgagcccac ccatgattca ggggccgact ttgcaggtcg tccccgcgct
     6961 caggatcgac gtccacggat aatacgaccc cggctcacac aggttgctcg cggcactgtc
     7021 gcatatatgg ccggtggctt gcgtctggtc ataacacccg gcggcgttgg cggaatcacg
     7081 aaacgtcgcc ctccaacaag catctttgct gcccatgccc tggtaattcg aagcggcggt
     7141 gcatgtttgc gacgcgggat catcgtatgc ggagacccaa tagtcgagtc cagcggagtc
     7201 tgcgcaccga accaagtccg ccacatacca actcgcgatg agttttcgag aaggcgggct
     7261 cgacggtgcg cattcggtct tcattgcggt atcgatgacg ttgccgtcgc acgaccccga
     7321 gaccttccaa gctccggatt cgcaggtggc cacggcgttt cccattcgtt ttttgggttg
     7381 gttgggaacg acggggaccg tggcaccatg cggatgagag ccggagggaa gggtggcgga
     7441 acaggtataa tttccagcgg ttttggggtt accgttccat gcggattggg actgactcca
     7501 cgtgacattc acgcctccag gacaagaggc gttacaaacg ccgttgacgc aagttccacc
     7561 ggggcaaggg gtcccggcgg atttggcgat gtgtacgcat tgtccggcac cgtcgcattg
     7621 gtcgatcgta caagcgttgc cctcatcgtc gcacggcgtc ccactaacct tatacggata
     7681 gctgcacaat ccgctcagcg agtcgcagat gccggaggaa acgaagcatc cgttgggagg
     7741 tgatgtgcaa gagacgggag tccctccctc acattgtcct gcatggttgc acaggtcgcc
     7801 cacggtgcaa gcatcaccgt cgtcgcacgc ttcgcctggt gtgcaggtag gatttgctcc
     7861 ggagcctccg ctgcctgccg ttcctccggt ccctgcggtg ccgccgtcca cggcgacgca
     7921 ttgaaactga atttgcgtgt tgccacaggt cattcccatg ggacaggagc cgcagtcgag
     7981 cacattgccg caaccatcgc tgatgattcc gcaggcggca cccaattgga tacacgtttt
     8041 aggcgtgcag gcatggatgc cacaacggtt tgtgccgcct ccgccgcaaa cctgtccatg
     8101 ttcgcatcca ccgcatgcga tggttttccc acaaccgtcg gcgatgacac cgcattcggc
     8161 atgcgcggat aggcaggtgg ccggttgaca tgctccggct tcggcggcgg catcctgtga
     8221 agtacccccg cccgaacctg cttgcgcgtt ggtcaccgag ctttcgcctc ccggaccaca
     8281 agcaacaaac acgaatggcg cgaaggcgat cgcccacgaa aaaatcttta tcatggtgtc
     8341 atatccgttg ccgtttcaaa gcgtccatca tgattccaag catgaggtcc aatcatagca
     8401 tttgtggttc gttgtcgagc catgcgttcc gaggtggcgc ggcgcccctg gtgaaggtat
     8461 cttcgtccat accccattgt ctccgcccgc gaccggacga ggacaacgtg tcatgatcgc
     8521 gaaaggaggg ttcgtcgtgc gtattcaact tctattggcc gctgcactgg gtctttccgt
     8581 tctttccctt ccccacgacg tctgcgcgga cgaatccgcg gaggcgcctg cgccttccct
     8641 cgagggcacg tatgctccgc agaacccgaa agcggctcag gccgtcattg acaatgccat
     8701 cgaatcggtg gtgcaggaga tgaatttcat caaacggccg ttcgctcgct ccagacttcg
     8761 cgataccaat ccgctgctca cccaggtccg ctttcaacgt caagatgatc tgctcatcta
     8821 tacgtacaac gcgggtaatg tcatccgact cgttcccaat ggtcctgcca tccgatggaa
     8881 gtcccccgcg gatggaaata cctacgaaac caaagcaacg ctcgtggggc gtcgattcac
     8941 cctctttttc aaaggcgagg aagggagcaa aaaagaagtc ttcgagcttg gggccgatgg
     9001 aaccacgctt actgttcata gcgtgatcac gagcccccaa ctgcccaaag ccctggagat
     9061 tcgctacacg ctcaagcgca agtaatcgaa agcaacgtgt gtgcgcatcg acgcgttcga
     9121 agtcctgcat caggcaggtg aatcggtgtt gcgacggccc agtcccacga tcgccgttta
     9181 cgggtagcca ctggcatcgt tttcggtctt cgtggcagag aatgatttct gcctaaccat
     9241 atacggatag tcggtggatg gctacgaagc ccccctcggg gggagggttt tggctttgtc
     9301 gccgtcgttc atggttcggg acaagaatcg gcttttgtgg tgggaaggga caatcacacc
     9361 ggaaagcacg ccgagaccgg caacttccgc ttcgatgcgg tcccccgccc gtaacgagcg
     9421 acgcccatca ctcgatgttc tcgccaaggg gccggaagcg atgatgtccc cgggagcaag
     9481 cgcagccgcc tcgctcgcca aagctaccat ttgcggaaat gatgttcgcc aaggcttgct
     9541 cggcgcttgt acgaccgtct gcccgttgac ccggatgctt acggtccacc gggaagggtc
     9601 ttgcgcttgg ttggcgacga gcaccgggcc aagctgaccg agccgaaaac tgccccaccc
     9661 gggcgatacc cggttgcgcg acggatagga ccagagactc aacagggtaa atccgccaat
     9721 ggcgcgatcg gattgttcct cggtggcatg atcgagttcg tcgatgagaa tggctcccac
     9781 ctgaaccgaa agttgaggag aaggttcgtc gtcagaaatg ggcagcggcg cctcatgccc
     9841 gcgcaaacac cggctatttc cccagcgaaa ccgtggcacc tcatcttccg ccaacaccga
     9901 aaactccgcg agggcagggg agtcgaccgt cggtggacga taaagacaca aggtttggtc
     9961 caacactgcc tgccgaggtc ccggaatatc acccaaggat tcggcgagtt ctatcagtcc
    10021 cgccataccc aacgaaaaca ctcgctgccg atactcattg gcgcgatcgg tgaaatgcgt
    10081 aggagaccat tcgagcgcaa aacactcttc gagtttacga acactgaaga gttctcccag
    10141 gtgctcgatg gcaatgagag gttcaccggc ataaacgaca ttggcgaggc gcaagctggc
    10201 catcgtcaca cctatctaat tgaatttaca ctattttatc agttttcttc atggctcgag
    10261 ggcggccgcc cacgaaatcg tttaccggga accgagccac aaatccgctt ccctcgaggc
    10321 gaaagcctgc cgcatggatg ctagcagacg atcggtgcgt tgtcgccccg ttgacgcgac
    10381 gcgagatggg cttagaatgc cgcgtcgtcc acggccggac cacgatggat tttcggtcta
    10441 gcacaggacg ataacgtcct tggcatgcgc ggcgtcgcgt ccccttgggc cccaggaatc
    10501 gaaggagcac gccgtgagta atcggaccaa gttcatcttc ctgaccggcg gcgtcgtgtc
    10561 cgccattggg aagggactga cgggagcctc cattggggcg ctgatggaag cacgcgggct
    10621 gcgtgtgacc aatatgaaac tcgaccccta tctgaacgtc gaccccggaa ccatgtcgcc
    10681 gattcagcac ggtgaagtat tcgttaccga tgatggcacc gaaacggatc tcgacctcgg
    10741 acactacgaa cgcttcaccc aaaccgtcgc cagccgaagg agcacctgct cgtccggaag
    10801 catctacgaa gcggttttgg cgcgtgaacg tcaaggcggc tatctgggcg ccaccgtgca
    10861 ggtcattccg cacgtcaccg acgagatcaa aaatcggatt cgcaatgccg caagaggtta
    10921 cgatatcgct atttgcgaaa tcggtggcac catcggcgac atcgagtcct tgccctttct
    10981 cgaagccatc cgacaaatga gggtagaact cggcaaggaa aacaccatca gcattcacct
    11041 cacgctcgtt ccctatattg ccgcggctgg cgaactcaaa accaaaccca cgcaacactc
    11101 cgtcaaggaa atgcgcgaaa tcggtatcca acccgatatt ctcgtctgcc gaaccgaccg
    11161 ccccctcgat aagtccatca aaggaaaaat cgcgctgttt tccaatgtcg atgccgattg
    11221 cgtcatcacc gccatggacg tggcattcat ctacgaattg cctctgttgt tgcatcacga
    11281 gggaatcgac gtccggatca cggagcagtt gaatatttgg gctcgttcgc cggacctgga
    11341 agcttggaaa cgcgtgtgcg aacgatacaa aaatccaacc caaggaacgg ttcgcatcgg
    11401 tgtcgtcggc aagtacgtcg acctgcgaga ctcttataaa tcattgagcg aagcgctcgt
    11461 acacgccggt ctcgtcaacg acgtgcgtgt ggacctggaa tacatcgcca gcgaaaccat
    11521 cgacagccaa aatgtgcagc agattcttgc cgggctcgat ggggttctgg ttcccggcgg
    11581 cttcggagaa cgcggcgttc gtggcaaaat cgaagcgatt cgtcatgccc gcgaaatgcg
    11641 tattcccttt ttcggaatct gtttgggaat gcaactggcg gtcatcgaat tcgctcgcaa
    11701 ccaattgaag ttggaggatg cggactccac cgaattcaat aaacatacca ggcacgccgt
    11761 catcgacctc atgcccgatc agcggaatgt cacggataaa ggggcgacca tgcggctggg
    11821 agcctatccc tgcgtcctga aaaaaggcac gatggccgcg cagatctacg gcgctaccga
    11881 aatcagtgaa cgccatcgac atcgttatga attcgccaat gcctaccgcg agcccatgga
    11941 agctgcgggc atggtcttgt cgggcttttc acccgatagc aggcttgtcg aagtcatcga
    12001 actgcccgat catccctttt tcatcgcctg ccaattccat cccgaattcc gaagcaaagt
    12061 gatgaaagcc catccccttt tcgtcaattt cctccaagcg gccatgaagg cccggacaca
    12121 aaacgactcc acccaatctg ctgcaccatc caatccgaca aacgaacagg cttgaacgtc
    12181 agaacgcatc ttgcacaaag ccacggcgga gcacgaccat gacagaacta gaacacgaat
    12241 gcctcgaccg ttttcctcat tggctcgatt gcctccacga agacgtggtg gcgctcgcgt
    12301 cgttgctgac cgcggaatcc attcccgaag gtgcgcaacg acatatcgcc ggcgccctca
    12361 actacctcac caaatccctc gacctgattc ccgatggcgt ggaagacttg ggttacctcg
    12421 atgacgcatt cgtgattcgg gtggccgcaa gcttggccgt ggcggaaacc ccgtccgtgc
    12481 gtgaagtcga tattcgcggg ctgttggttc ggctcgcgca ggaagccaaa ctcatagaac
    12541 aatggatggg aaatgacttc ccccgcctcg tcaaagtcgt gcgcgagctt ggcaaaggcg
    12601 cttcgcgcgg ccggagcgtc gatgatatca tcaccgaccc ggccattcgc gccgaagcca
    12661 tccgcgaagt gcaatcctgg tcgaaaacct atcaaaagcc gtcgttccac aaggacccac
    12721 agacgctcat caaacttcga tcgtttctgt cgtcgagatt accctgaccg gagtcgtcat
    12781 cgaacgtctt gcgagcgcgt ggggcgatcg gccgatggcg gcatgagttc gttcatgagg
    12841 tctctgacct ccggtttatt tttcaagtgg gcgatgagca agccgcagac ctcggaaaat
    12901 gagcgctcga acgcgattcc ttcccgagtg cgaaccgcaa ctttttctcg tcccgcggcc
    12961 aggtcttctt ccaaggcttc cgcataacgt accaattgat tgcgaagttc ttcgatttgg
    13021 cggcgaaggt cccgtgcgac gacctcccgg gaggcacgca actgctgcaa cttttctacc
    13081 tgttcatgca gcagcgtggc gcgagcgatg gcggcaccgg cgcgttcgaa gatggctcgg
    13141 ttgaaagtgc gtgttttttc agcagcatcg gcggcatgct tggcggcagc ggcttctcgt
    13201 tctgccgtgc gcatttccgt agccatccgt tcgagttctt cttgatgttt ggcaacctct
    13261 cgcaacgccc gcgattcttc gtgcgcaagt tcttcgactt ttcgtccgat ttcggcacgt
    13321 aacccgcgac ctcttttttc aagcgcctcg agcttgcgcg tatgcgtggc tacctcgcct
    13381 tccaaacggc tagcccttgc cgccaggtcc caagcttttg cgactccttc ttccagttcg
    13441 cgaggcgcgt tgccttgatg ataagccctc gccaccattc gcgaaaaaag cgccgcgcga
    13501 ttggcccact ccgtaacccc ttccgattga ggaggaggcg gcggaggagg cgccggtgtc
    13561 tctccttgcc cccccacgtc ccaattgcgc gatggcagcg aacgctgtag cgctttgagt
    13621 tgctcctgca ggtgatgcgc atccaaaaat cgcttacgag gatctttttc gagcaaacgc
    13681 agcatgatcg attcggcggc agggggcagg tccggacgca atttgctcgg acgaggcgcg
    13741 ggggcgtttc gctgcatctc caaaagcgtg tcccgatccg gcgacttgaa tggaagctgg
    13801 ccgatggaca tctcgaaaaa aaggacccca agcgcatata gatccgactt gggtcccgct
    13861 tcctcgcctc gcgcttgctc cggcgacata tattccgggg tgccgaatac cgcgcccttg
    13921 ggagcaagcc tcggatcgcg cgccaatccc gccaaaccaa aatccaatat cttgaccgag
    13981 tctttcctcc cccccttgac cgtgagcatg atgttgtcgc tcttcaagtc acgatgcacg
    14041 acgcccaaat catgcgcacg cgccaacgcc gcacacatct gctcgagtat gtcgaccgcg
    14101 cgaaccaccg gaaaaggacc cctggctatc tcactggata gcgaaatccc aaccagatac
    14161 tccatcacca aataaagctc cccctcgtcc gtctccccaa tgtcgtggat gtcaacgata
    14221 tgcgcatgat ccactcgatt cgccgctcgc gcctctcgca gcatccacgc ccgtagatgc
    14281 gtctcgcccc gcagatccgg gcgaattagc ttcaacgcca caacgcgatc gatgagcaca
    14341 tgacgggcgc gatacacgac gcccatgccc ccttcgccaa tgcgtgtctc cagacggtat
    14401 cggcctgcga tgaccttacc caggttttta tcgctacttt ttgctgtcat ggatgtcgtt
    14461 ggttgcattc ctgtagcacg tgaattcttg ggctatcaga cgttctcgag gctcatttcc
    14521 gctgtcgtgg gcactctgtt ccaaaacgat caaaacgtgg cgtgaaacac cggaaggcga
    14581 agctccgttt tgggttcgac cccgtatttt ttcgtctcta acgccgaata cacgggaaga
    14641 acctgcaccg ccggtacccc cacctgaaag gcgccgtcat ggatgtccac caaagacgtg
    14701 ggaacatacg gtctcgatgc tacaggcgta gactttttcg gggaagcttt ggcttccgga
    14761 gccgtcaccg tcacctccgt tcccggcgtc tgcggtcccg tcggaatctc ctccggcccc
    14821 gtatccgctc caggttcgtc cgccaaataa tgatcgggcg ctttgtagga cgtcgcctga
    14881 agtatcgcca ccgttgtcgg aatgagcagc gccaaccctc ctgcaagcat gtagtaggca
    14941 ggttgcggat cgcttccctg ttcgacgaaa tagcctccca caccgccaac agccgcccct
    15001 gccacgcctc cgcccacgta ggcccacgtg ttttgaaccc ccaccgccgc ttccacggcc
    15061 atgaccaact ctccgcctaa cagcgcccct cccgtaattc ctttgcctgt cgcatcaatt
    15121 cccgcttctt cggccatagc ccctcgactc acggtggcga tcgccaaggc cgcaaatgca
    15181 acggttgtgc gtaacgttgg gttatgcatg gtctattccc ctctgcaaat tcatggatgg
    15241 tcttccttag gcattaaaag cgacgacggc aagattagca cgcaaccaag ccctctccta
    15301 ccacggagaa gccgtcgaag gatatcgatt cgatgtggtc tcaactacgt gtattgccta
    15361 cctgccatgg cagtttccgg gtcccattgg cttactccgg ctccaactgc cgcgaacggc
    15421 acgaagactc ctgggtgtca tgggtggttt ccgacgaacc cattggatcc tggttcaccg
    15481 aacggtagca tcccacggac gtttccctcc ggaggtgtac catgttcgat gttttgctca
    15541 ccgagcaaga aagagcggtc cgggatgaag cacgggcgtt cgtccgggac caagtggacc
    15601 cgcaactgct gcgggatatg gacgcggata aagtcgagta ccccaccgaa tttgtgaaaa
    15661 acctcgccaa agccaatctt ctcggtttgc gcttcgatcc cgcgtacggc gggcgtgcct
    15721 tgagttgggc cgcggaattc gccgttctcg aagaaatcgg cgtgctcggc actgccttgg
    15781 gctgcgccta ttccatgccc agcatcgtcg gtgaagccct cagccgtttc ggcaccgaag
    15841 aacaaaaaca aaaatacctc ggccccatcc ttcgcgcgga actcatctcc gccgaaggac
    15901 tcaccgaacc ccgcggtggc agcgactttt tcggcgcctc caccatcgcc agacgcgatg
    15961 gtgacgacta cctgctcacc ggacaaaaac gcttcgtcgt cggggctgtc ggtgccgata
    16021 tcttcctcat ctacgcgcgc accggcgatg ccaatgccaa accccataaa gccatctccg
    16081 cgtttctcgt cgaacgctcc atgggggtgg aggtcaaaca cgtgtacgga ctcatgggca
    16141 cccgaggcgg aggcacgggc cgcatcgttc ttcgtgatgt gagagtgccc gctcgcaatc
    16201 tcattggtcc gctgcacgcc ggatacgacg tgttcaaccg aatgatgatt cctgaacgac
    16261 tcacctccgc cggtggcgcc atcggcatgg gacgaagcgt gctcgaagtc gccacgcgct
    16321 acgcacacaa acgaaaagcc ttcggtcggt tcattcgtga ctttcaaggc gtaagcttcc
    16381 gtatcgccga atccgtgacc cgtctcgata ccgcccgggc gctctgcttt gccgcaggaa
    16441 aaaccgtcga atccggtgtc gatgcccgtc gtatcgtctc cgaagccaaa aaagtcgcca
    16501 ccgaagccgc ctgggccacc gtcaacgatg ccatgcagat catgggagga atcggatata
    16561 cacacgtttt cccggtggaa cgcatgcttc gggacgttcg cctcgcgctc atctggaccg
    16621 gcaccaacga aatcatgaac ctgctcgtgc aacacgaata cgccaaagaa ttgctcggcc
    16681 gttcgttcaa agtccgagat gtgcagcagg atgccgtggc tccggaacac gtcgacgaaa
    16741 tcgtctatga atgactctag cggggatcat ttaaactgct tataattcaa taggttacgg
    16801 ctgcttcgaa gggctggggg ccgaggaaac cgcgggagcc acggaagccg aaggcgtcgg
    16861 ctgcgcggag gatttgcccg tatccgcagg gggagctttc gaggcggaat catcgtcttc
    16921 gtgaatgatg atccggattt cgcgctgctc tgtgccgcgt tcataggcca ccacgatttg
    16981 gggtgcggtg gccaacgatt gccaaacgcg aaatgcctcg tcgtaatggc cgatgggaga
    17041 tgcattgacg ctcgtgatca catcgcccgc gcgcaggtcc gtgccttgcc aaaagtcggg
    17101 gtcgccggtc agggccacga tctggaagcc gtaaaatcgg ccatcgacca ttttttcttt
    17161 gacatccagg cgttgaagaa acgccccgag cccaggggat aacacctcca tgacttgata
    17221 ccgccacaca tgccctttgg gcagtgcgct cggctccgga agcggaggcg gcgcctcggc
    17281 ggttttcgga gtcaccggcg cgggcgtgtt ttcggcacac gaaaacgtcg ccattccaag
    17341 caccatccac aatccccaaa aaaatgacct ttgaactcgc accctccctg cttatcgcta
    17401 gacgcgatcg gtggccaagt cttcccgcct ccgaggagac cttgtcatca cactatcgct
    17461 tgtctccccg aaaacgacgt acctcggcaa cgattcgttc atgcacggtc tgctcatcgg
    17521 gattgccatc gatatggacg acaagatccc ggggaaccca tgcctccgcc gtctcataag
    17581 ctttcgccaa cgcttcttgc aatacgctgt cttcatataa ttccgtcgag tacgatcgct
    17641 gctcgcgccg acgtgccgcg atctcagggc gaacgtcgag aacgatggtc aagtccggac
    17701 gcctcgctcg gccgttgatc gtttgtaccc actcgacgac ggaagggtcg acctctgccg
    17761 cgctcgtgat ggattgatac gcaatgctgg acagatcgaa gcgatcgctg atgacggtca
    17821 caccatccat caggttgcgg acaatcaagg aatcgagatg atcgagtcgg tccgcggcaa
    17881 agagcaaggc catggttgcc caggttggcg cgcgcggtcc tgcgatgcca cttaccacca
    17941 ggcgatgcgt gagcacttgg cggatcatcg ctccgatagg cccttccgag ggttcgcgcg
    18001 tgaccagaac aggtaagccc tggttgcgat accattgccc aagaagagcg gcttgcgtcg
    18061 atctccctga accgtcgatt ccttccagaa cgatgaattg accttcgatc attccgcttg
    18121 tcctttgata ggaggatcct cgccaagcac gatattgtca atgagtcgtg caggacccac
    18181 gcgaatggca agcgcaatca aggctcggtg agcgatatgc cgctgctcat ccatcggctc
    18241 gagcgtatcc gcatccgcaa tctcaacgta atcgatgcta tccgcgaccg gtgcgacaca
    18301 cgatacaacc tcgtggcgca acgaggccac ctttcgttca cctcgttcgt aggcgcgaac
    18361 ggcgcggtta agtccacggg gaatcgctgc cgcacgctgt ctttgctctt cattcaagta
    18421 acggttgcgg ctgctcatcg ccaaaccatc cgcctctcgt accgtcggat ggcccacaat
    18481 cgtgaccgga agcaataagt ccgtcgtcag cctctcgatg accttgatct gttggtaatc
    18541 cttgcggcca aacaccgcca cacacggtcc ggtggccgca aaaagtttcg tcacgatggt
    18601 ggcgacgcct tcgaaatgcc ccgcgcgaaa agctccacaa agcggctcgg cgatcgcgcc
    18661 aacccgtact cgcgtctgct cccccgccgg atacatggtg ggcacggatg ggtgaaatac
    18721 gcaggaagcg ccgtgggaaa tgcatccctc cacatcgctt tcccacgtgc gggggtactt
    18781 gtccaagtct tcggaaggac caaattgtgt cgggttgacg aaaatactga caaccacgaa
    18841 atcggcatga cgacgggcct cgtcgatgag cgcgaaatgg ccgtcatgca aagcccccat
    18901 cgtgggaacc agcccgacgc gcttttcttg cgcgcgcgct cgatcacaag cttgtcgata
    18961 cgtggatagg tcggtgtaaa tgggcagcga tcccgagatc atgcgtggat ccaacgcgga
    19021 ggcgagacga attgcaagag gcaggcgcga tgatcagctc gaacaattcg cctcccacaa
    19081 cccctggtag tactgagcca tgaacgcata ccaggggctc atgggcgcac atatcgagta
    19141 ggcgcattgc aaggctcgga tcgtataata ctgcgccatc ccctgataat aggagcaaag
    19201 cggattcgtg gaatacaagg acatggtcat cgcttgctcg acctgcgccg gtgatgtgaa
    19261 cggcccatgc tgccgcgcca acacaatagc gtccgcaacc gcgggctcga cgtcatgggc
    19321 gagaagatcg tcgcgcgaaa actcgttggc aatttgaagg atggtggcct ctgggctttg
    19381 tgtcgcatca agggaagcca acgcttcgcc ggccaccgtt tcctcttccg gtgcctgcgc
    19441 gcaagccatg aaggtcagac tgagcaaaaa aacgaagaac tttcgcatgt cgaactcctg
    19501 aaacgcggcg gttccaagcc gcgtcgagcc cgaatgcatc gacgatgcca atgacgacga
    19561 tgcccacgag gagctaacgt tgttgctgct tttcgtaggc catgggagca aggcggcacc
    19621 tttgcggata cgacaaggca aagccaagtg acggtatgtc gtgacctggc caaaaaccga
    19681 tagtcatccg gcgtttcggc gttttgcgat cctggctccg gttcgtcggc aggatcatag
    19741 aaaaaacaat gtaaattcaa ttagttacaa atatagggga gatggcgtag ggattggatt
    19801 gcgggacagg aaagacaaaa ggcgaaaggg ctcgagaagg gtttcgaacg ccgaggattt
    19861 cattctgggc gcacgccatc ggctggacgg agccgggcgg catagagcga ggggatgacg
    19921 gtggcaatga ggcagagggt cagggcaatg gcgccggtga tgatgaattc ggtggggcgg
    19981 acttcgaccg gcaaggtgga gatgaagtag actttggggt cgagggggaa gccatagacg
    20041 agcagacctt tgcagacggc caggcccaag ccgaggccca gcgcggtacc catgaccccg
    20101 atgataccgc cttgataaac gaaaatccgc aaaatcgcga catcgcttgc ccccatggct
    20161 ttgagtacgg cgatttcctt tttcttttcc aatacgagca tgatgagtgt ggcaatgacc
    20221 gtgaaggccg ccacgaagat aatgagtgcg agcacggcgc tcatgccgat tttttggatt
    20281 ctcaaggcgg tgaaaaggcc gtggttcagg tcttcccaat ccatggtgct gtacaagttg
    20341 gaggacagac ggctggcaac gtccagctcg atttttcctg cttcatcgat gtcccgcacc
    20401 ttggcttcga cgcccgtgac gctgtcgccg tagtcttgaa agcgttgggc ttcatacagg
    20461 tccacgtaca cgagcttgga gtcgtattga tcgaatccgg cctcgaagat ggcgatgact
    20521 ttgaattgtt tggcgacggg agggcgaatc acgcctccgg aatacgagaa accgatggtc
    20581 ggggatgtga cttgcaagca gtcaccgacc tgaacggata ggttttgtgc gagggtattg
    20641 ccgacgacga tgccgggcag ttgtgcgacg gcttccgggc tattgcacgg gtcgggtgcc
    20701 acgtcttcgg gaagatcgtc ttcagggtcg gggagttggc ttttgtagcc gccttcggga
    20761 acgaccgagc ccaccatgac atcggcggcg accccggcgt cgagagtgtc ggaagaagga
    20821 tctagcgggg gcggttggtg ttccacttcg aggatttcgt cgattccgtc tgggggaccc
    20881 tctggtggtt tgggcttggc gttgcgctcg tcttctcgga tcgaatcctc gatggctcgc
    20941 agcaacgtga tgtttcttcc ggcgtcgtcg cgttcggttc cgccatcctt cggttccgcg
    21001 gttttggatc ccaagggcaa gggtttgggg gcgggggtga tgcctccgcg cgtttttcgt
    21061 tctggtggcg tcgcgcccgg ctttcgaagc ccgtgcaacg agcccccttc tttgatgtga
    21121 ctcggcaaat cgagcacggt cggcatcaat tcgggatcga cccccttgac gagcacgccg
    21181 gtcgccgttt tttccttgtg ggagaccatc atggggttga tggtgaacgg agcaactcca
    21241 atgattccgg gtacttcccg catcatggcc atgatttctc ggtactcacg gaaatccgag
    21301 gagtacttga gcacgagcac gtgagcgttg accccaagca cttttttccg aaactcggat
    21361 tggaaaccgc ccgtgacact catgaccgtg agcagggcgg ctacccccaa ggtcacgccg
    21421 agaatggcga aagcagtgcc gacagagacg aaagcacctt tggacttcgc gccgaggtag
    21481 cgggtggcga tttgcaacgg gtaacccatg ctggcgattc tgttagcagg ggaagccaac
    21541 caaagcgagt gtcgtgtgtg tgaacgtgtc ggtccgtgcc tggggcagca ctccatgggc
    21601 ttgccaatcg gtcgcaccac cctatcttct gagcctcaat cgcccccttc gaacggagaa
    21661 cctgatgacc aaagttgcga tcatttatta ttcttccacg ggaacgaacg aagccatggc
    21721 tcgggcagtc gccgaggggg ccaaggaagc gggagctgaa gtaagagtcc gtctggtggc
    21781 cgaaaccgcg cccgatgcgg ccatcagccg caatcccgca tggcggtcct tcgtcgatgc
    21841 ccatcaaaac gatccccggg ccagcctcga cgatctcgaa tgggctgacg ccatcatttt
    21901 cggcaccccc acccgtttcg gcaacatcgc ctcccaactc aaagccttca tcgacaccac
    21961 cgctggactg tggctgcaag gcaagttgtc caacaaggtc tacgcggggt tcacctcggc
    22021 ccaaaatgcc catggtggtc aggaatccac cctgcttgcc ctgttcaata ccgtctatca
    22081 ttacggcggt ttcatcgtgc ctccggggta tacggacccc cttgtttttg cctctggagg
    22141 caatccctac ggtccttccg tcaccgttgg ctccggcggc cccgtcggac aagcagacct
    22201 ggctcacggc cgctacctcg gaaaacgagt ggcggaagtg gcgagcaaac tggtggcctg
    22261 ctgatggtac ttttgggtgt tttgttgggc cgggttgccg cgcccgtcga ctcctactag
    22321 gaaaggtggg tccgcgtgga ccaactcccg gaacgcgttt tcgggatcgt atcgaggaga
    22381 tcatatcatg agcttgaaac ctggtgtcgt caccggagac gagtacaaga agctagtgga
    22441 tgcagccaag gctggtggat acgcgttgcc cgcggtcaac gtcacgagca ccaacacgct
    22501 caatgccgtg ctcgaagccg ctgccaaggc caaaagcgat gtcatcattc aactgtccaa
    22561 cagcggtgcc caattctac
//
LOCUS       DAOSOR010000021        22368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_2325_length_22368_cov_4.296464, whole genome shotgun sequence.
ACCESSION   DAOSOR010000021 DAOSOR010000000
VERSION     DAOSOR010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 22368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22368
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2325_length_22368_cov_4.296464"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            462..1163
                     /locus_tag="PK710_02135"
     CDS             462..1163
                     /locus_tag="PK710_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08534.1"
                     /translation="MVRPHRSAHAGPAESKDDGGGEMGLGREDYGHGSIPNEPNGLEP
                     SDGVGRGGERRKPIELGLRRDLTHLWTQPEHGPPKASGSGSTTGGLQEALDAHDQKIG
                     LGFGGPILSAFHAVASSEIAPRSGAATFEAIIDASGRVAEVLVLSGDNDLRAWTALAR
                     AVHASLRSRVLRAPRKKGVAVVVDVRIEHRLPSGALPDKRMDVNSGAVAFDLADVGAR
                     AVRQVSVRILSEYRL"
     gene            1223..2497
                     /locus_tag="PK710_02140"
     CDS             1223..2497
                     /locus_tag="PK710_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08535.1"
                     /translation="MRSCILHAVCGLGICLGFLGCTLDREGTGGEPDAGMGAGQSTGG
                     ASTMDASAGGTGGFDGSAGTQQGGQGGAAGIATGGTGGIQAGGSAGEPGGSGGGLSDE
                     VCDDGIDNDGNGLVDCEDGACKAVGYRCVPAVPEEWEGYFWIHVRPYQGEDEETSCPG
                     GEKANRYYSRNAEKAICDPCACSPLEGTLCDAPGIACDYKSKDCANAVPQPDLENFDC
                     HNLSGSSISCQLTGNNPIAKLGSCAPIDEVPDFPNKFPFQDAVDLCAAPPNANTHGCD
                     SQICMPPGEGGFAGAICIMQESEQSECPPQWDAENPLRLHLLENAIDTRSCSPCSCTV
                     DGDSIDCEGGSYKVYDDDGCKSCNGIFCDDEMIVSTTECINLSKLADWDTISIRANAR
                     PTPKNGKCRVSGGVAQGSVQTMKGVTLCCVSP"
     gene            complement(2752..5388)
                     /gene="mutS"
                     /locus_tag="PK710_02145"
     CDS             complement(2752..5388)
                     /gene="mutS"
                     /locus_tag="PK710_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOT08536.1"
                     /translation="MSGDKGKDTEKLTPVMRQYHAAKQAHPDCILFFRMGDFYEMFYD
                     DAVVAARELDLTLTSRNKGAPDEVPMAGVPHHAAHGYIARLVSTGFKVAICEQMADPS
                     KTKGIVPREVVRVITPGLVTHEDQLEARSNNYLCALDASDSRFGLALLDVSTGEMLAT
                     TLEDTAAIMAELARVEPREVLLEQGHDDLRTALTQILPTTSFREDPPLEQNNVPSILE
                     QALAGEAAAEASDSLSCAALRACARALRMALGCNPGQSLPVQRIVAFDSSGVVQIDEV
                     AQTHLELVRSIDGSKRATLLSVVDETVTAPGARLLRKWILSPLLDMRALRRRQDAVEA
                     FVQDPVARTELRTIFKNIGDIERLAVRATLREITPRDLGVLRDSLIAVPQAFHIVSQM
                     TDPSVHENLLKDGMDPTLLELRDLLLSALVERPPPTVRDGDIFREGFDEELDETGKLQ
                     REGADMIIALEADLRASTAIPTLKVRYTRVFGWYIEVSKTHASKVPHGWRRKQTVATG
                     ERYTNDALDDLADRLLHAEERHSQRESLLFSELVQHIAERASALRAVGHQIATWDAFA
                     SFAEIAHRFDYCRPIVEESDTIDIRDGRHPVVERLAAAGRFVPNDVCLQGDKQRLWLI
                     TGPNMAGKSTLMRQVALIVLLAQTGSFVPASQARIGMVDRILSRVGANDNVSRGESTF
                     MVEMRETASILRQATCRSLVILDEIGRGTSTYDGLAIAWAVAEHLHDVIRCRAMFATH
                     YHELTELAETSPHAVNVSVSARERGDDVFFLYKLVPGAASRSYGIAVARLAGVGESVL
                     ARAKAILSSLESGGALPGGRYATLRGRSASGSVQLDLFSPQPPQPEPSNPAVETLKQV
                     DPNRLSPLDALQLVIKLKEMIG"
     gene            complement(5402..7879)
                     /locus_tag="PK710_02150"
     CDS             complement(5402..7879)
                     /locus_tag="PK710_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08537.1"
                     /translation="MTRGEVAVIDLNEETVVDLNPSKPGFNFIPVGAVPVDIAATPGG
                     TAAFVGSAEFGREGIWIVPTRTVVQGNPHLTSFAACALPSAPGEMIVLTQPTPDIISP
                     SKCNAEPYDDPDHPQGDLALEHGVSGTLKLVTTLPDLGAVVVMDAQQLIDQPPGSFRA
                     CPIERWVSLQVDLPPALPQQRVPEGGFPPGESADGSVCAMTQRPDIPTASNPLPRPSG
                     LAFDPSSSRLFIADEDAPVIHVLDASSPCELSESSPLLPMSVVRPERKVVTRSLAVSP
                     TTSAGKKYLYATDLLDGSVMIFDVSLQSTDRTPLLRPYPWRNPFQPLDRLSFSVPVRD
                     VEFVLRDRPVTDPSAGATAVGLACDPTHDDSLGARYRTSSDFSSGARPYNLRGVFATI
                     ALTNGQIMIVDVDDFDAACRRPKTACDGDEQASFEGASGELSCLMVQPHQPRSSAFLH
                     LSNDANGRVPGLQSYPVLSLGTSILPTDQTADGVRYPKLLAPSAPGIPLMVGGRVADE
                     PQTDPLIAEKNSVVFDLREPRVHVEQNWSVAFEGAIPGFEGHVGRLGPASEDGRTTFF
                     DGGAFFCDRGVHDFDAALTIGKNEGISDPATMQTWARDHQDVLQITEDFLLEDDPYWD
                     SVADHCSWLQCRETFGTVRDPKSTRDLPITEAYQGSLVVEGVWDFVRCCFPTLVSYTV
                     RPRNQWVVTGTAAGFLHRVVPDPFTGRCVDSCDPNRSLLNARVLERERSEPVPALDDP
                     GVFRNPALQFVLWRGTAPSERKMAFTFVQKDGFYPLFINLAAATSFVQPQSLTLAPTG
                     ELAIADGSAQGLILVDLGSIRVSRSFF"
     gene            complement(8134..9618)
                     /locus_tag="PK710_02155"
     CDS             complement(8134..9618)
                     /locus_tag="PK710_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08538.1"
                     /translation="MVRRRLAYSLLSLAVVTSCSCVSPGEGPFPASDAMYFPVGLAVS
                     PGGHTLYVANSDFDLQYNSGTVIALDLDRVRSNVPRVWEANAPCGSLGQNTMKLLYPG
                     PCGPISLHHPPDGQGSLVAASVRIGAFATDMIAVKRPDAPASRLFLPVRGDPSITWID
                     VEDDTDLEPPFHRVLHCGGERCDARHRAGENPNTNLRRTVLPPEPYGIAVSESRDVVV
                     VTHQTTGSVSLVSNAWDGIPTLEFVSGGFPIGGVGVAALPVPRYVTVSQSDYQPGFLA
                     TFRASPEIDLIRYFDDRAAAPARPFLVRAGVAPIRVNASGYDSRGIAIDPTDRRACEA
                     ECQDDLACLQACASVPVQVYVANRTPPSLLVGETRSNVSPTASDDLVTVFDTIPLTYG
                     ASRVLLGSVVNTAGQSERRVFVSCFDARFIFIYDPVTRRIDGQIRTGRGPHAMVLDPH
                     EPLLYVAHFTDSYIGVVDLDQRHATTYPSIIATLGIPLPPRESK"
     gene            complement(9818..9991)
                     /locus_tag="PK710_02160"
     CDS             complement(9818..9991)
                     /locus_tag="PK710_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08539.1"
                     /translation="MLPAFIIDQIRRREREQERRGEQQPVLEVPTPSAPGRSEDADDE
                     EDKERGVLIIDLG"
     gene            10163..11449
                     /locus_tag="PK710_02165"
     CDS             10163..11449
                     /locus_tag="PK710_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HOT08540.1"
                     /translation="MVALLLAPAARAVPYDFDGDSWEGCSVFLDVLRDKVGRNNVVAT
                     SELRWENLNPRDALVVIYPLRELNADELSSFLRAGGRVAILDDFGASEANLKRFQIER
                     IPAPSRPARTLRNNPALAIAEPVADMVAGVPAGVHPAVADVVSIVTNHPTALTHPNLT
                     PTLRIAAMDEPDGIVAVAGQVGRGRLFVVGDSSVVINQMMRYPGNRAFVQGLGAYLSS
                     DDTWGERKGTIYVVARSFQETGTFENRSSVGKSLGAAGRAFRRWLAETRAAGLPSSVI
                     WALGMIASVISLLWVGKMAARTYPRRSPRFAWPTPLVAQGGVAGRAAVLTAPSTHKAL
                     ALLEIKSAMEESVAARFDLPLPLSTPDLFETVERSGALELSELKQFKHLLLTLSTVET
                     SVAAGTPLRVRERDVQAAIQKLHHLLARIDEKGSSR"
     gene            11451..12437
                     /locus_tag="PK710_02170"
     CDS             11451..12437
                     /locus_tag="PK710_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOT08541.1"
                     /translation="MTESPDGRTADMNEIAAAKERLEDMRRQVLRVFIGSSRVTDLLL
                     TALLAKGHVLLEGVPGVAKTTLAKAFSAALGCTMRRIQFTPDLLPADITGTYVLSPKE
                     GTFSLRPGPVFANLVLADEINRAPAKTQSALLEAMQEQQVTIEGDRFELPKPFVVVAT
                     QNPIDLEGTYPLPEAQIDRFMLRVAVGYPTHKDEVLMLQTHNAASPRAHAVLSADDVL
                     ALQNIAARVHVEEDLFDYVVNLSGFTRMHARVALGASPRASLALVQAAKGAAVINGRN
                     YVTPDDIRSLAGPVLAHRLILVPEVEGDDSVREQVVEEALSKVSYRRAVRAV"
     gene            12444..14003
                     /locus_tag="PK710_02175"
     CDS             12444..14003
                     /locus_tag="PK710_02175"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOT08542.1"
                     /translation="MHIYPTRPAVHVAFAGAVVLLAGVIGQRPPIIAWGAAILIALVF
                     ARAAALVSVSRIRNAGFEMVWSEEKKVVATVRGAEVELAAEVRNRDNLAARYVHLRPV
                     ASSHLLVSLEPTMGEVPAGGRLHVTVRVQTPRVGRHAIHGLSLEVQGAPGLFEVPLTF
                     ANPYGIEVLPRPFSPLLLSARGGRSRREAELGRPGPMRGDGVDLRELRELLPGDSFKR
                     IAWRASARRGKLIVREFEHDERDVVWLVLDASVEHWAGVVGKAPLDYGIDEVAAAARK
                     HLGRGDSVGLAVFGGRLRGLLEPDRGPRHAHHIALALTLSTATYDADRSELEEREVAA
                     RVLEHIRPLEPKFGRIKKYDLDRLAFHAEAAMSRAPFRATTPEAPTQREQSLRKYLAA
                     FGIESPARSDSEKIRTEEAMNRFFGRLAKRKPRPSLVHVWAPGPDYAKPEFVQALSQL
                     RRVGATIRWTTPRVELPADPRDSVMAQAVTQAVLLRSHTARKRAERVLARMGVRMQRI
                     RRYLPAESESP"
     gene            14000..14887
                     /gene="rsmA"
                     /locus_tag="PK710_02180"
     CDS             14000..14887
                     /gene="rsmA"
                     /locus_tag="PK710_02180"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOT08543.1"
                     /translation="MKPFDPPRKNSLSARQGFRDRGFHAKRSFGQCFLADQNIARRIA
                     QHSVPQPGGTVLEIGAGTGALTGPLLQRQAIVVAIERDRDLIPLLEESFFQPIADGQL
                     FVCEHDAATSDWLALLSEHPTPHAVVGNIPYQITGRLLQRATQVACHVQRVVFMMQKE
                     VADRIQAPCGSRQYGALSVFVRRSFRVVERISVPPTCFRPQPSVHSAVLVLEPHPTLP
                     PADPIFDELVSAAFSQRRKTLRNAWRGSMGLSVQDWEIIAPDAGINLDARAETLCVQD
                     FERLASRLRQVREKHAEGL"
     gene            complement(14934..16202)
                     /locus_tag="PK710_02185"
     CDS             complement(14934..16202)
                     /locus_tag="PK710_02185"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HOT08544.1"
                     /translation="MIPSNMRGFIVFPCLVIVGCATSRPEYHANPEEPDGDASVDAPF
                     DSSSHDGSGDSQPDGPAIEAGQWPGICVDGDVEECYTGPATTKDVGACKHGTHVCSDG
                     KYPPTCDGQILPTDEQCNGIDDNCDGTVDEGCACSNGDTVPCYGGPPNTEGVGPCKGG
                     TQTCVNGLWGACQGAVLPIPETCNGIDDDCNGSIDDKTGLRLFSMPFDGQAWSSQFLS
                     DAWKGANAPPACANVMAAVQLTDIGRLLVFTDDGMLYWRKNGVWMAPIFATGRFPSLP
                     ATIDSVYQVPYSVFGPNLGATLTFSAVPYAYLYDYDINDSVTKADDFPVIMTDDTSKG
                     GPNQKDVRARWSVERFDPSQYQQSADALLLYREYDDGKVYQFNAAFEWKSWSVAQSPL
                     WQGKANAPPTAKCVAGFADHDPDKAYLICP"
     gene            complement(16305..17468)
                     /locus_tag="PK710_02190"
     CDS             complement(16305..17468)
                     /locus_tag="PK710_02190"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOT08545.1"
                     /translation="MTKRNLIRKIGVVLGVSTLVLVGGCTQKKKDSATEGASSAEVSG
                     QGPRIELVDPVHDFGTVTEGETVKHTFVIKNTGTSPLVIERVRSSCGCTAAVTKESEV
                     GPGKTTEVEVSFNTRGRMGPNSKTITLQTNDKNTPSAKLEIKAMIEQLLAFQPSIVRL
                     AVGHNEEQTMDAWLTGKLAKDAKLTLGEVTGDTGVKIALTERKEGEESHVGVRFTFKG
                     TKVGRGNGTVSVKTGVESMPELTLRFDTTVMGNLQLRPRALYFDTQSPQGKERVLNIT
                     SKRDDLKVTAARVVEGPYEAKVTKPETGPGFEVHVTLKAAGDKESPDANLNGKLEIVS
                     NDPLEPKMEVPLHVRRMPNLIRPRPSLSMQREFPRRLNIMGNPPEKPEAPPAQ"
     gene            complement(17529..17975)
                     /locus_tag="PK710_02195"
     CDS             complement(17529..17975)
                     /locus_tag="PK710_02195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOT08546.1"
                     /translation="MNLVSHHDKVLVHYTGRRLDGSIVGSSSQTGAVELQLGNGSVPP
                     GLERALVGMRVGEKKSTLLRAKEAFGLRQKDLILRLDARNFPKETCPKVGQVLKMQNH
                     DGAMLHVQVTEVTENEIVLDGNHPLAGLDVTFEIELIRIVDGNPPS"
     gene            complement(18033..19328)
                     /locus_tag="PK710_02200"
     CDS             complement(18033..19328)
                     /locus_tag="PK710_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08547.1"
                     /translation="MKHLPRNVKTRSSRESLCGVDEADEPFGWDESYSVTPPPVAVSP
                     TPSEPIAQTVPCSSGKSAMCGAVLLGIVIALLVTWVKVPRQEPAMTLGSWVHDLPQLA
                     SSTMVQVDSAQLNSLFRQRRADAALEHAGYASIPGGVLYTPESFRSSDGSYDLLIHFH
                     GNVKVVVESAAVSKLNAAIAVVNLGVGSGPYQDAYATPGTYENLLAAIQSAVQRRGLE
                     NARLRRVALSAWSAGYGAISTILQQRRGEDPLDALLILDGIHTGYEQGRPGTLVERRL
                     ASFDEAAEAAASGELLFSVTYSEIDPPGYAGSRATAEHLLSVAEKRGEVKDSALAVPA
                     YRKLESMRGAVAKEDVRKLEPYADQQVGSFRIRGYRGNTRGHHMAHLFQMGATVLPEL
                     AVRWDAYAGYAGRRDAVRSSAVRQRARGKQLVQHRQSPP"
     gene            19505..19768
                     /locus_tag="PK710_02205"
     CDS             19505..19768
                     /locus_tag="PK710_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08548.1"
                     /translation="MWRFSPIAIALPFMLTVGCAGSQQQPAAPEGFKVCSYCCTTANT
                     VCRCEVIEAVECPAPDQTATLPACAECRSCTKRLESGESCQPQ"
     gene            19794..20240
                     /locus_tag="PK710_02210"
     CDS             19794..20240
                     /locus_tag="PK710_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08549.1"
                     /translation="MKPKTLAILIAALVAAVIAALVVWDIVVVTDQERLEAFADLVTS
                     EVSPENIEAAMAYVDPAVQPIVVEVRHQTLRFDKDNRDDFSLMARGRLRPYHGIKQHV
                     MRKSVDVRDSQASINTETFSRQGRVSVDWELRKRGEDWWVSRVALR"
     gene            complement(20243..21001)
                     /locus_tag="PK710_02215"
     CDS             complement(20243..21001)
                     /locus_tag="PK710_02215"
                     /inference="COORDINATES: protein motif:HMM:NF021539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HOT08550.1"
                     /translation="MPTSFDPNSAPADCHQETAVFHTAIAHLYRAEMNRMTVWRQRLD
                     TTSNWAILLTFGMSTFVLGTTSVPHYVMLLGLAIVSICLVIEARRYQRLHHSKWRLAL
                     FDHNYYANMLWPSARLAEPGWRRQLAFDLRRPHFTMSWYMATRLRLRRNYLLLVYFIT
                     GVWLTKVFIHPHSPVSVWDYYQRLRVEGLFPCWFVAVTATFFLVMTSVMGLLTPSEEA
                     LDRWTWERQAVRPEPPSEEALAAEDARKTPAPAP"
     gene            complement(21430..>22368)
                     /locus_tag="PK710_02220"
     CDS             complement(21430..>22368)
                     /locus_tag="PK710_02220"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOT08551.1"
                     /translation="LVDPHPASDIKPVPDFFLDGKASPNATHGFVRRKLPHGSMLAFT
                     RAFEYQGRRYLLADDLSAVPADRVLIYKPTSFRGVTITRELLPHFGWTCASPTARFAL
                     QDGKMVATGQSFAARHLVRLTPQTERIRGREYRKLRDDSHWVLAHQVCEVNLVDNRPE
                     GIADDEPWIYVSTLERTLVAYEGLRPVFATLHASGRGGVHRGKGDVRNYTTPLGSFPI
                     NWKERYATFSPDPGAPTTFWISQVMFVQYFEQPYALHGAYWHERFGIPTSAGCPNLSP
                     YDAERLFTFTQPTLPDGWQAIAPHRNERGTLVVVGR"
BASE COUNT         4809 a         6306 c         6524 g         4729 t
ORIGIN      
        1 ccacggcaac ggtcaaaggc aatctaggct cccagaaaga gcccacggat ggttcccctc
       61 gcgagccgac gacagctccc gaaattcttc tcgacctcgg ttccaatggt gccatctggg
      121 attgcgtcac caatagcgct ccgggtggct gagtccgccc attctttgaa ccgtatgcct
      181 cctcgaggca tcccatgttc gccttgtcca acccctttgc gcgcgcgctg gccacggcca
      241 ttgtgcttca tatcgcggtt ttcgccgcac tggcgcattg ggctagccgt cgtcccacgc
      301 ccccggccat tatttcggtc cgttctccca cttggatcac gacgctagac ccttgggttg
      361 aagttgatgg ttcgccaact tcttcgctgc cctcgacttc cgagacacgg tactctccat
      421 ccaatcccaa taacccattg gattcgtcga cgcctcgggc gatggtacga cctcatcgta
      481 gtgcgcacgc tgggccagcc gagagcaaag acgatggggg cggcgaaatg ggactagggc
      541 gggaagacta cggtcatggt tcgataccta acgagcctaa tgggctcgaa ccatcggatg
      601 gagtcgggag gggaggggag cgtcgaaagc ccatcgaact tggtcttcgg cgcgatctta
      661 cccatttgtg gacgcaaccg gagcatgggc caccgaaggc ttccggctcc ggatctacga
      721 ccggaggctt gcaagaagcg ctcgatgcac acgaccaaaa aatcggtctc ggttttggag
      781 gacccatttt aagcgctttt catgcggtgg cctcgtcgga gatcgcaccg cgctcgggag
      841 cagcgacctt cgaagccatc atcgatgcgt ccggacgtgt ggctgaggtt ctcgttttat
      901 ctggagataa cgacttacgc gcatggactg ccctggcccg agccgttcat gcgtcgttgc
      961 gctcccgtgt cctgcgcgct ccgcgaaaga aaggcgtcgc ggtcgtggtc gacgttcgga
     1021 tcgagcatcg gctaccaagt ggcgcgttgc ccgataagag gatggacgtc aactcaggtg
     1081 cggtggcttt tgatctagcc gatgtggggg ctcgtgccgt gcgtcaggtt tccgttcgta
     1141 ttctgtcgga atatcgactc tgatggttgt aaaaacgcct catcggcttg ctatacaacg
     1201 aggtttcgat aatcattcat gaatgcgctc ttgcattttg catgcggttt gcgggctggg
     1261 tatctgtctg ggttttctag gctgcacgct ggatcgtgaa gggacagggg gagagcctga
     1321 cgctgggatg ggggctggac aaagcacggg tggcgcttcc accatggatg cttccgcggg
     1381 agggacgggc ggcttcgatg gaagtgccgg aacgcaacaa ggcgggcagg gtggagctgc
     1441 gggaatcgcc accggtggta cgggagggat acaagcaggc ggttcagcgg gagaaccggg
     1501 tggatccggg ggaggcctaa gtgacgaggt ctgcgatgat ggtatcgaca acgatggaaa
     1561 tgggcttgtg gattgtgagg atggggcatg caaggcggtc ggataccgct gcgtgcccgc
     1621 cgtgccggaa gaatgggaag ggtatttttg gattcatgtg agaccatacc aaggtgaaga
     1681 cgaagagact agctgcccgg gaggtgagaa agcaaaccgg tattattcaa gaaatgctga
     1741 aaaggcgatt tgtgatcctt gcgcatgcag cccgctcgag ggcacgttat gtgacgctcc
     1801 gggcatcgct tgtgattata aaagcaagga ttgcgcgaat gccgttcctc aaccggattt
     1861 agaaaacttc gattgtcaca atctatctgg gagttccatc tcctgccaat tgacaggaaa
     1921 caatccgata gccaagctgg gatcgtgtgc gccgattgac gaagtccctg attttcctaa
     1981 taaattccca tttcaggatg ctgtcgatct ttgcgcggcc ccacccaacg caaacaccca
     2041 cggctgcgat tcgcagatct gtatgccgcc gggagaggga ggttttgccg gagcgatatg
     2101 tatcatgcag gaaagtgaac agtcggagtg tcctccgcaa tgggatgccg agaatccttt
     2161 gcgtttgcac ctgctggaaa acgccatcga cacccgttct tgcagcccct gttcgtgtac
     2221 cgttgatggt gattccatcg attgcgaggg gggaagctat aaggtgtacg atgatgatgg
     2281 ctgcaaaagt tgcaacggta ttttttgcga tgacgagatg atcgtgtcga cgacagagtg
     2341 cattaatctt tcgaaattgg cggattggga tacaatttcg attcgggcca acgctcgacc
     2401 aacccccaaa aacggtaaat gccgcgtcag cggtggagtg gcgcagggaa gcgtgcaaac
     2461 catgaaaggc gtcaccctct gctgcgtttc tccttgatag tgccgtctcc gccaagaaac
     2521 aaacctcgaa tcgtcggttt caaggctacc gaaccctact tcatgccagg gctgtcgctt
     2581 cctatgtcat gggcgtcggc catcgttgga caataagtca aaccagaaat agatggccat
     2641 ctataagtag gttgcccaaa gaactcgccc aagggcggca attcattgaa attactggct
     2701 atttctggcc gtaggatact cgaatcgcgt cgatggcgca ttgctttccc ctcatccaat
     2761 catttctttg agcttgatga ccaattggag agcgtcgagc ggggagagcc gattcggatc
     2821 gacttgcttc aaggtttcga cagcagggtt ggagggttcg ggctgtggcg gttggggaga
     2881 aaagaggtcg agttgcacgg aaccggaagc gctgcggccc cgtaaagtgg cgtagcgtcc
     2941 gccgggcagg gcgcccccgg actcgaggga cgataaaatg gctttggccc gagccaacac
     3001 ggattcaccc acgcctgcca ggcgtgcgac ggcgatgccg tagcttcggc tggccgctcc
     3061 gggaacgagt ttgtacaaga aaaaaacgtc gtcgcctcgt tccctggcgg agacggatac
     3121 attgacggca tggggcgacg tttccgccag ttcggtcaat tcatggtagt gggttgcgaa
     3181 catggcgcga catcggatga catcgtgcag atgttccgca acggcccaag caatggccaa
     3241 gccatcgtag gtactcgtgc cacgcccgat ttcatccagg atgaccaacg accgacaagt
     3301 agcctgccga aggatggaag cggtttcacg catctcgacc atgaaggtgc tttcgccgcg
     3361 agagacatta tcgttggcgc cgacacggga cagaatgcgg tcgaccattc cgattcgagc
     3421 ttggcttgcg gggacgaacg atcctgtttg cgcaagcagg acgatgagcg ccacttgccg
     3481 catcaacgtg gattttccgg ccatattggg tccggtaatc agccacaaac gttgtttgtc
     3541 tccttgcaag caaacgtcat tgggaacgaa acgccccgcg gcagccaggc gttccaccac
     3601 gggatgccta ccatcgcgaa tatcgatcgt atcgctttct tccacgatcg gacgacaata
     3661 gtcaaagcga tgagctatct ccgcaaacga ggcaaatgca tcccatgtag cgatctgatg
     3721 ccccaccgct cgcaaagccg atgcgcgctc ggcaatatgc tgcaccaatt cggaaaacaa
     3781 caatgactcc cgttgggaat gacgttcttc cgcgtgcagg agccgatctg ccaagtcatc
     3841 caacgcgtcg ttcgtatacc gttctcccgt cgcgacggtt tgtttgcgac gccaaccatg
     3901 gggaactttg gatgcgtggg ttttcgatac ctcgatatac catccgaata cgcgggtgta
     3961 gcgcaccttc aaggtgggaa tggccgtgga ggcacgaaga tccgcttcca aggcgatgat
     4021 catatccgcg ccttctcgct gcagttttcc cgtttcatcc agctcctcat cgaacccttc
     4081 acgaaaaata tccccatccc gaacggtggg gggaggacgt tcgacgagag ccgagagcag
     4141 cagatcccgc agctcgagga gcgtgggatc catgccatct ttcagaaggt tttcgtggac
     4201 ggatggatcc gtcatctgcg acacgatatg gaacgcttgc ggtacggcaa tgagcgaatc
     4261 gcgcaatacg ccaaggtctc gtggtgtgat ttcgcgaagg gtagctcgaa ccgcaagccg
     4321 ctcgatatct cctatgttct tgaaaatagt gcgtaattct gttcttgcca cggggtcttg
     4381 cacgaacgct tccacggcat cttgtcgacg acgtagggcg cgcatatcga gcagcggaga
     4441 cagaatccat ttgcgaagga gtcgagcccc cggcgcggtg accgtctcat ccaccaccga
     4501 tagaagtgtg gctcgtttcg atccatcgat cgagcgaacc agttcgaggt gggtttgtgc
     4561 aacttcgtcg atttgcacga cgcctgatga atcgaaggcc acgatacgtt gaaccggaag
     4621 cgattgacct ggattgcatc ccaaggccat gcgcaacgct cgggcgcaag cgcgcaacgc
     4681 tgcgcaagac aggctgtccg atgcttccgc cgctgcctcg cccgcaagag cttgctcgag
     4741 gatcgagggc acattgtttt gttcgagcgg gggatcttcg cggaaagatg tggtgggaag
     4801 aatctgtgtc aatgccgttc gaaggtcatc atgaccttgt tcgagaagga cttcccgtgg
     4861 ttcgactcga gccaactcgg ccatgatcgc ggcggtgtct tcgagcgtgg tcgcgagcat
     4921 ctcgccggtg gagacatcga gcaaagcgag accgaaacgc gagtccgaag catccaacgc
     4981 gcataaatag ttgttggagc gcgcctcgag ttggtcttcg tgggtgacca gtccaggggt
     5041 gatgacgcga acgacctcgc ggggcacgat gcccttggtc ttgctcggat cggccatttg
     5101 ttcgcagatc gcgaccttga atcctgtaga aaccagtcta gcaatgtatc cgtgggcggc
     5161 atggtgcggc acgccagcca ttggtacttc atcgggcgcg cccttgttgc ggctcgtcag
     5221 cgtaaggtcc aactctcgcg ccgccaccac tgcatcgtcg taaaacatct cataaaaatc
     5281 acccatccga aaaaaaagga tgcagtccgg atgagcttgt ttggcagcgt gatactgccg
     5341 catcacgggc gtgagctttt cggtatcctt ccctttgtcg cccgacatcc gcgcggacct
     5401 ctcagaaaaa ggatcgggaa accctgatcg accccagatc cacgaggatc aatccttgag
     5461 cggaaccatc cgcaatggct agctcgcccg tgggagccag tgtcaacgac tggggctgaa
     5521 cgaaactcgt ggcagccgca aggttgatga acagcggata aaatccgtct ttttgcacga
     5581 atgtgaacgc catcttccgt tccgacggcg ctgttcctcg ccacagcacg aactgcaacg
     5641 cgggatttcg gaacacccca ggatcgtcga gagccggaac cggttcactt cgctcccgct
     5701 ccaacactct cgcgttgagc aaggaacggt tgggatcgca tgagtccacg caacgacccg
     5761 tgaacgggtc gggtacgaca cgatgcagaa atccagccgc tgttcccgtc actacccatt
     5821 ggttgcgagg acgcacggta tacgatacca gcgtggggaa acagcatcgc acgaagtccc
     5881 aaacgccttc caccacaagg ctaccttgat aggcttccgt gataggaaga tcgcgcgtcg
     5941 acttggggtc acgcaccgtg ccaaacgttt cccgacactg gagccatgag cagtgatctg
     6001 ccacagaatc ccaataggga tcgtcttcca acagaaagtc ctcggtgatt tgcagcacgt
     6061 cttggtggtc gcgcgcccag gtttgcatgg tcgcgggatc ggaaattcct tcgttttttc
     6121 caatagttag agcggcatcg aagtcgtgga ccccccgatc gcagaaaaac gctcccccat
     6181 cgaaaaacgt cgttcgccca tcttcggaag ccggaccaag acgacccaca tggccttcga
     6241 aaccaggaat ggcaccttca aacgctaccg accaattctg ttcgacatgc acccggggct
     6301 cccgaagatc gaataccacg gagttttttt cagcgatgag cgggtcggtt tgcggctcgt
     6361 cagccactcg tccgcctacc atcaaaggaa tccccggcgc cgatggcgcg agcaatttcg
     6421 gatatcgaac cccatccgcc gtctgatccg taggcaaaat actcgtgccg agcgatagca
     6481 cgggatacga ctggagaccg ggcactcttc cgttggcatc gttggatagg tgaaggaatg
     6541 cactactgcg tggctgatgc ggttgcacca tcaggcatga caactcgccc gacgcgccct
     6601 cgaagctagc ctgttcgtcc ccatcgcaag ccgttttggg cctacgacac gccgcatcga
     6661 aatcgtcgac gtccacgatc atgatttgcc cattggtcaa agcgatcgtg gcaaatacgc
     6721 cccgcaggtt gtacggcctg gcaccgctgg aaaagtctga tgatgttcga tatcgagccc
     6781 ccaacgaatc gtcgtgggtg ggatcgcagg cgagaccgac agccgtggct ccagcgcttg
     6841 gatcggtaac gggtcgatca cgaagtacga attcgacatc acgcacggga accgaaaaag
     6901 aaaggcggtc caggggctgg aatgggtttc gccacggata cggacgaagc aatggcgttc
     6961 gatcggtgga ctgcaggctc acgtcgaaaa tcatcacgct tccgtcgagc aagtcggtgg
     7021 catataagta tttttttcca gccgatgtgg tggggcttac ggcaaggctt cgcgtgacga
     7081 ctttgcgctc tggcctcacg accgacatgg gcagaagtgg tgaagactcc gaaagctcac
     7141 acggagaaga agcgtcgagc acgtggatga ccggcgcgtc ttcgtcggca atgaaaagcc
     7201 ttgacgaaga aggatcgaaa gcgagtcctg atgggcgagg caatggattc gatgcggtgg
     7261 ggatatcagg acgctgcgtc atggcgcaaa cgctgccatc tgcgctttcc ccaggaggga
     7321 aaccgccttc aggaacgcgt tgctggggta atgcgggggg aaggtccact tgcaaagata
     7381 cccagcgttc gatgggacac gctcgaaaac taccaggtgg ctgatcgatg agttgttggg
     7441 cgtccatcac cacgaccgct cccaaatcag ggagggtggt tacgagtttc agcgtgccgg
     7501 agacaccatg ctccagggcg agatcgccct gaggatgatc ggggtcgtca tacggctcgg
     7561 cgttgcattt cgatggcgag ataatgtctg gcgtgggttg cgtaagcacg atcatttcgc
     7621 ccggagccga gggtaaggcg catgccgcaa acgaggtcag atgcggattg ccttggacca
     7681 cggttcgggt gggcacgatc cagatacctt cgcgcccgaa ttccgcggag ccgacgaaag
     7741 ctgcggttcc ccctggcgtg gcggcgatat cgacagggac ggcaccgaca gggatgaagt
     7801 tgaatccggg tttcgatggg ttcagatcca cgactgtttc ttcattgaga tcgatgaccg
     7861 caacctcgcc gcgtgtcatc tgggtgacga aagcgtggag cgtccccgga tcctcacttg
     7921 aagaattcct aatctttccg caatgttgca taggtcgacc cgggtgttcc cggtcgttac
     7981 gggacaggca aagaaacgct acattgccgg attgttcgag cgagcgcacc gcgacagaca
     8041 ccggttgctc agagcaagac agggctagcc cgccaagcat cgagcctagc aagactcgtc
     8101 gtctgaacga ttggcgagaa tccacacgag cgatcacttc gattccctcg ggggtaacgg
     8161 aatacccagc gtcgcaatga tactgggata tgtggtggca tgacgctggt ccagatccac
     8221 cacaccgatg tacgagtccg tgaagtgcgc cacgtacaac aagggttcat gcgggtcgag
     8281 gaccatggcg tgtggaccac gaccagtccg aatctgacca tcgatacgtc gtgtcaccgg
     8341 atcataaata aagatgaatc gcgcatcgaa acaggaaacg aaaacacgac gttccgattg
     8401 tcccgcggtg ttgaccaccg agccgagcaa cacccgcgaa gctccgtagg tcaaaggaat
     8461 cgtgtcgaaa accgtcacca aatcatcgct ggccgtaggg cttacgttgg accgggtttc
     8521 gcctacgagc agggaagggg gcgttcgatt ggctacatat acttgtacgg gcacgcttgc
     8581 acaggcttga aggcaggcga ggtcatcctg acattcggct tcacacgcgc gtcgatcggt
     8641 ggggtcgatg gcgatacccc gggaatcgta accgcttgca ttgacacgaa tgggagcaac
     8701 acccgcgcgt acgaggaaag gacgcgccgg tgccgccgcc cgatcatcga aatatcgaat
     8761 caaatcgatt tcgggggatg cgcggaaagt agccaaaaaa ccaggctggt aatcgctttg
     8821 ggataccgtg acataccggg ggacgggcaa ggccgccacg ccgactccac ctatggggaa
     8881 ccctcctgat acgaactcta acgtgggaat gccgtcccat gcattgctca cgagactgac
     8941 agaacccgtc gtttgatgcg tgaccacgac gacatcacgg gattccgaaa cggctatgcc
     9001 gtaaggctcg ggcggaagaa ccgttcttcg caaattcgtg tttggattct ctcccgctcg
     9061 atgccgtgca tcgcaacgct ctcccccgca atgcagcacg cgatggaaag gcggctcgag
     9121 atccgtgtca tcctccacat cgatccacgt gattgatggg tcacctcgaa ccggaagaaa
     9181 taagcgcgaa gcgggggcgt cgggacgctt gaccgcaatc atgtcggtgg cgaacgcgcc
     9241 gatacgaacg gaggccgcga cgagtgaccc ttgaccatca ggaggatgat gaagcgatat
     9301 cggtccgcat ggccccggat acagaagctt catcgtgttt tgcccgagcg agccacacgg
     9361 tgcattcgct tcccatacac gcgggacgtt gctgcgcact cgatctaggt ccaacgcgat
     9421 caccgtgccg ctgttgtact gcaaatcgaa atccgaattc gcaacatata gcgtatggcc
     9481 tcccggtgat accgcgagcc caaccggaaa gtacatggcg tcggaagcgg gaaaaggacc
     9541 ttcccctggg gagacgcacg aacacgacgt cacaacagcg agcgaaagaa gcgaataagc
     9601 gagtcgacgt cgaaccattg cgcggcaccc tagtgagacg cgggagccgt gtccagacgc
     9661 cattttccga ggcaaggtcg aagctgggtt caaagcttcc tgaccgggga aacggacgat
     9721 agaatcacaa cgtcgtctcg ggattgacgg cgtaggcttt cgcggggcat cttgccatga
     9781 tgactcatcc ctcgaggacc ttatctgctt tgattgctca accgaggtcg atgatcaaca
     9841 cgccacgctc tttgtcttcc tcatcgtccg catcttccga ccgaccagga gcagacggcg
     9901 tgggcacttc gagcacaggc tgctgctcgc cgcgtcgttc ctgttctcgc tcgcgacgtc
     9961 gaatctggtc aatgatgaac gctggaagca cggtggtctc tccggccttc tcgtatggtt
    10021 cacaagccac tgggggctct aaaaagtcac tctagcaatc gccaatctac ggtcaagctc
    10081 agtgcaaacc tacaaccatc gacatgattt tcctgcttgt tgcaaacgat ggcagccttg
    10141 gctgacgagc accgttttgg ctatggtagc actgctcctc gccccggccg caagggcggt
    10201 cccttacgat ttcgatggtg attcgtggga aggttgctcc gtttttctcg atgtgctccg
    10261 agacaaagtc ggccggaata acgtcgtcgc gacctcggaa ctgcgttggg agaacttgaa
    10321 tcccagagac gcgttggtgg tgatttatcc acttcgtgaa ctcaatgccg atgagttgtc
    10381 ttcttttctc cgagctgggg gacgcgtggc catcctcgac gacttcggcg cctccgaagc
    10441 caacttgaag cggtttcaaa tcgaaaggat tccagcccct tccaggcctg cccgcacctt
    10501 gagaaacaat cccgccttag ctatcgccga acccgtcgcc gacatggtcg cgggtgtgcc
    10561 cgccggtgtg catccagccg tggctgacgt tgtatcgatc gtcaccaacc atccaacggc
    10621 tttgacccat ccgaacctca ccccaacctt gcgcattgct gctatggatg agccggatgg
    10681 tatcgttgcc gtggccgggc aagtcgggcg aggaaggctt tttgttgtcg gtgatagctc
    10741 ggtcgtgatc aaccagatga tgcgatatcc cggaaaccgt gcttttgttc aaggattggg
    10801 agcttaccta tcgtccgacg atacctgggg ggaaaggaaa ggcacgattt atgtcgtggc
    10861 acggagtttt caggagacgg gaacgtttga gaatcgttcg agcgtgggca agtctctggg
    10921 ggcagcagga cgggcttttc ggcgctggct ggccgaaacc cgcgccgctg ggttgccctc
    10981 ttccgtgatt tgggctcttg gcatgattgc gagcgtgata tcgctgctgt gggtggggaa
    11041 aatggctgca aggacgtacc cgcgccgcag tcctcggttt gcgtggccca cacccctcgt
    11101 ggcccaaggc ggtgtggccg gacgagccgc cgtccttacg gccccctcca cacacaaagc
    11161 ccttgcattg ctcgaaatca aaagtgcaat ggaagaatcc gtggccgccc gcttcgactt
    11221 gcctctgccc ctgtctacac ccgacctctt cgaaacggtc gagcgatcag gtgctctgga
    11281 actctcagaa ttgaaacaat tcaagcattt gctgttgacc ctgagcacgg tggagacgag
    11341 cgtcgctgcc ggtactcccc tgcgagttcg agaacgcgac gttcaggccg cgattcaaaa
    11401 actgcatcat cttcttgcgc gtatcgacga gaaaggctcg agccgctgaa atgacggaat
    11461 ctcccgatgg tcgcacggcg gatatgaacg aaatcgccgc agcaaaagaa cgactggaag
    11521 acatgcgtcg ccaagtgctg cgtgtattca ttggctcatc gcgcgtgacg gacctgttgc
    11581 tcaccgcact gctcgccaaa ggccacgtgc tgctcgaagg tgtccccggt gtcgccaaaa
    11641 cgacactcgc caaagccttc tctgccgccc tcggatgcac catgcgtcgt atccaattta
    11701 cgccggacct cttgcccgcc gatatcaccg gaacctacgt cctttccccc aaagaaggca
    11761 cattctctct gcgcccagga cccgtgttcg ccaatctcgt gctcgccgac gaaatcaacc
    11821 gagcgcccgc gaaaacccaa tccgccctgc tggaagccat gcaagaacag caggtcacca
    11881 tcgaagggga ccgcttcgaa ttgccaaaac ctttcgtcgt cgtggccacc caaaacccca
    11941 tcgatctcga aggaacatac cccctgccag aagcgcaaat cgaccgattc atgctccgcg
    12001 tcgccgtcgg ctatccaacc cataaagacg aggtcctgat gctgcaaacc cacaacgcag
    12061 catctcctcg tgcccatgcg gtcctgtccg cggacgatgt gttggcgctt caaaatattg
    12121 ctgctcgcgt gcatgtcgaa gaggacctct tcgattatgt cgtcaatctc tcaggcttca
    12181 cacgaatgca cgctcgagtg gccttgggcg cctccccacg cgcttccctc gccttggtgc
    12241 aagccgccaa aggagcggcg gttatcaacg gacgcaatta cgtcactccc gacgatattc
    12301 gctcgctcgc cggaccggtg cttgcccatc gtctcatctt ggtgcccgaa gtagaaggag
    12361 atgactccgt tcgtgaacag gtggtggaag aagccttatc caaagtgagc tatcgtcgtg
    12421 ccgtccgggc tgtgtgagga aacgtgcata tttatcccac tcgacccgct gtccatgtag
    12481 ccttcgcagg cgcggtcgtg ctgctcgctg gcgtcattgg ccagcgcccg cccatcatcg
    12541 cttggggagc agcgatcctc atcgccctgg tatttgcccg cgccgccgca cttgttagcg
    12601 tctcgcggat tcgtaacgcc ggattcgaaa tggtctggtc ggaagaaaaa aaggtggttg
    12661 ccaccgttcg aggggccgaa gtcgagttgg ccgcggaagt gcgtaaccgc gataatcttg
    12721 ctgcacgcta cgtgcatctg cggccagtgg cttcgagcca tttgctcgta tcgctcgaac
    12781 caaccatggg tgaagtgcct gccggcggac gattgcatgt gaccgtgcgg gttcagacgc
    12841 ctcgggtcgg acgacatgcc attcatggtt tgtcgctcga ggtacaaggt gcgccagggc
    12901 ttttcgaagt gcccttgacg tttgcaaacc cctacggaat cgaagtcctg ccccgtccgt
    12961 tttcccctct tttgctgtcc gcgcgcggag ggcgtagccg acgagaagcg gaattgggtc
    13021 gtcctggacc tatgcgcggc gatggcgttg accttcgaga acttcgagag ctgcttccgg
    13081 gagattcttt caagcgcatt gcttggcgtg cttccgcacg acggggcaag ctgatcgtgc
    13141 gggaattcga gcatgatgaa cgagatgtgg tgtggctcgt gctcgatgct tcggtcgagc
    13201 attgggcggg tgtcgtggga aaagctccct tggattatgg aatcgatgag gtagcggccg
    13261 cggcgcgaaa acaccttggc cggggagata gcgtgggatt ggccgtgttc ggaggccggt
    13321 tacgaggcct tctcgagccg gaccgaggcc cccggcacgc gcatcatatc gctctcgcct
    13381 tgaccctttc taccgcaacg tacgatgcgg atcgcagtga actcgaggag cgagaagtgg
    13441 ccgcccgggt tctcgaacac attcgtccgc tggagccgaa gttcggtcgt atcaaaaaat
    13501 atgatctcga tcgcctcgca tttcacgcgg aagcggccat gtctcgcgct ccttttcgcg
    13561 cgaccacacc ggaggctccc acccagcggg agcaatcatt gcgcaagtat ctggccgcgt
    13621 tcggaatcga gagccccgcc agaagcgatt cagaaaaaat ccgaaccgaa gaggcgatga
    13681 atcgcttttt cggccgcttg gcaaagcgca aacccaggcc cagcctggtg catgtctggg
    13741 ccccgggtcc cgattacgcc aagcccgaat tcgtccaagc cctgtctcaa cttcgacgcg
    13801 tcggagccac catccggtgg accacgccaa gggtcgaatt gccggccgat ccccgcgact
    13861 ccgtgatggc ccaagccgtt acgcaagcgg tcctgcttcg ttcccacacc gcccgaaaac
    13921 gcgccgagcg cgtgctggct cgcatgggcg tgcgcatgca aaggattcgt cggtacctac
    13981 ctgctgaaag cgaaagcccg tgaagccgtt cgacccccca agaaaaaact cactgtctgc
    14041 aagacaaggt tttcgcgaca gaggattcca cgccaagcgt tccttcggtc agtgctttct
    14101 cgcagaccag aacatcgccc gacgcattgc ccaacatagt gtgcctcaac caggaggaac
    14161 cgttctcgaa atcggggcag gaaccggtgc attgaccggt ccgttgctcc aacgccaagc
    14221 catcgtcgtc gccatcgaac gagatcgaga cctcattccc ctgctcgaag aatcgttttt
    14281 ccaacccatc gcggacggtc agctattcgt atgcgaacac gatgccgcta cgagcgactg
    14341 gctcgccttg ctttccgagc accccactcc tcacgcggtc gtaggaaata ttccctatca
    14401 aatcaccggt cgcctgttgc aacgagccac gcaagtggct tgccatgtgc aacgcgtcgt
    14461 cttcatgatg cagaaagaag tcgcggatcg aatccaagcg ccttgcggat cacggcaata
    14521 cggtgccctt tcggttttcg tgcgacgctc gtttcgtgtc gtcgagcgca tttccgtccc
    14581 tcccacttgt tttcgtccgc aaccttcggt gcattccgcg gtgttggtgc tcgaacctca
    14641 tcccacgctt ccaccggccg accccatctt cgatgaattg gtgtcggccg cgttttctca
    14701 acgaaggaaa acgctgagaa atgcatggcg ggggagcatg ggattgtccg tacaagactg
    14761 ggaaatcatc gcccccgatg ctggaatcaa cctcgatgcc cgggcggaaa ccttgtgcgt
    14821 gcaggacttc gaacgattgg cctcgaggct gcgtcaagtt cgagaaaagc acgcggaagg
    14881 gctttaaatt tattcaataa aaacaataga ttgcaagaat gtcgccgcga cgtctaagga
    14941 cagatcaaat acgccttgtc cggatcgtga tcggcaaacc cagccacaca ctttgctgtc
    15001 ggcggagcat tcgccttacc ctgccatagg gggctttgcg cgacagacca gcttttccac
    15061 tcaaacgccg cgttgaactg ataaaccttc ccatcgtcgt attcccgata caacagcagt
    15121 gcgtcggcgg attgctggta ctgggaagga tcgaaacgct cgacagacca gcgagcccga
    15181 acatcttttt gattggggcc gcccttcgag gtgtcgtctg tcatgatgac tggaaagtcg
    15241 tctgcttttg ttaccgagtc gttgatgtcg tagtcgtaca aatacgcata ggggactgcg
    15301 gaaaacgtga gcgttgcccc caggtttgga ccgaataccg agtagggaac ttggtatacg
    15361 gagtcgatcg tcgcgggaag ggagggaaaa cggccggtgg caaagatagg ggccatccat
    15421 accccatttt ttcgccaata cagcatccca tcgtcggtaa agacgagcaa tctgccgata
    15481 tccgtgagtt gcacggcggc catgacattt gcgcaagccg gaggcgcatt cgcccctttc
    15541 catgcatccg acaggaattg tgaactccag gcttgtccat cgaagggcat ggagaaaagg
    15601 cgaagtcccg tcttgtcatc gatggaacca ttgcagtcgt catcgatgcc gttgcaagtt
    15661 tccggaatcg gcaatacggc tccttggcaa gcgccccaga gcccattgac acaagtttgt
    15721 gtgccccctt tgcaagggcc tacgccttcg gtgttcggcg gtccgccgta acaaggcact
    15781 gtatccccat tggagcaggc acacccttca tccacggtcc cgtcgcagtt gtcgtcgatg
    15841 ccgttgcact gctcgtcggt gggaagaatt tgtccgtcac aggtcggtgg gtactttccg
    15901 tcgctgcata cgtgcgtgcc gtgcttgcaa gcgccaacat ccttggtggt ggctgggccc
    15961 gtgtagcatt cttccacatc cccatcgacg caaattccgg gccattgccc tgcttctatg
    16021 gcgggaccat caggctgaga atcgccgcta ccatcgtggg aagaagaatc gaagggggca
    16081 tccacggaag catcgccatc gggttcttct ggattggcat ggtattctgg acgcgaagta
    16141 gcacaaccaa cgatgacgag acagggaaag acgataaacc cgcgcatgtt tgacggaatc
    16201 atagcacaag ggagatatcg tcgctcgaat gcggctccat gaggacgaaa aaatttacgg
    16261 cagggatagc gacaagagat cagggacgag gggtgaggcc gtcgtcattg ggccggagga
    16321 gcttccggtt tttctggcgg atttcccatg atatttaggc gtcgagggaa ctcccgttgc
    16381 atcgatagac ttgggcgcgg tcgaatgagg ttgggcatgc gacgaacgtg aagcggtacc
    16441 tccattttag gttccagcgg gtcattgctt acgatttcga gcttgccgtt gagattggcg
    16501 tctggggatt ccttgtctcc cgctgctttg agcgtgacat ggacttcgaa gcccggacct
    16561 gtttctggtt tcgtcacctt ggcttcgtag gggccttcca caacgcgagc agcggtgact
    16621 ttgagatcgt cacgcttgct cgtgatgttg agaacgcgtt ctttcccttg gggagattgg
    16681 gtatcgaagt acaacgcacg agggcggagc tgtaagttgc ccatgacggt cgtatcgaaa
    16741 cgcagcgtca gctcgggcat ggattccacg ccggttttga cggaaacggt tccatttccg
    16801 cggcccactt tggttccttt gaacgtgaac cgaaccccca cgtggctttc ctcgccttct
    16861 tttctctctg tcagcgcgat ttttacgccg gtgtctccag tcacttcacc caacgtgagc
    16921 ttggcgtctt tcgccagctt gcccgtcaac caggcgtcca tggtttgctc ttcgttgtgg
    16981 ccgacggcca atcgaacgat cgaaggttga aaagccaaga gttgctcgat catggctttg
    17041 atttcgagct ttgcactagg cgtgtttttg tcgttggttt gcagcgtgat cgtcttgctg
    17101 ttgggaccca tccgaccacg ggtgttgaac gatacttcca cctcggtcgt ttttccgggc
    17161 ccaacttctg attccttggt taccgcggcc gtgcatccac acgatgaacg gactcgctcg
    17221 atgaccaaag gtgaggtgcc ggtgtttttg atcacgaagg tatgcttgac ggtctctcct
    17281 tccgtaacgg tcccgaagtc atgaactggg tcgaccaatt cgatgcgcgg gccttgtccg
    17341 ctcacttcgg cgctgcttgc gccctctgtg gcagaatcct tttttttctg agtgcaccct
    17401 cccacgagga caagagtcga aacacccaac acaacgccta tttttcgaat caagtttcgt
    17461 tttgtcatgg caacgacggc attctgatcc tccgcgatgc cacttgcaag ggggatgtca
    17521 acgatgcttc agcttggcgg gttcccgtcc acaatccgaa tgagttcgat ctcaaaagta
    17581 acgtccaaac cggccaatgg atggttgcca tccaaaacga tttcattttc agtcacttcc
    17641 gtgacctgga cgtgaagcat cgccccgtcg tggttttgca ttttcagcac ttgccccacc
    17701 ttcggacagg tttctttcgg aaaattccga gcatccagac gaagtatcag gtctttctga
    17761 cgtaaaccaa aggcttcttt ggcgcgcagc agcgtggatt ttttctctcc tactcgcatg
    17821 cccacgaggg ctcgttccag cccaggaggg acggagccat tgccgagttg caactccacg
    17881 gctccagttt gcgaagaaga accgacgatg cttccgtcga gacgtcttcc cgtgtagtga
    17941 acgagaacct tgtcgtgatg ggaaacgaga ttcatgggtc gctggaaatg aaaagatgaa
    18001 ccgtccgaag cgtggggccg aacaaccaac agtcaagggg ggctctgccg atgctggacg
    18061 agttgtttgc ctcgggctcg ctgacgcact gccgatgagc gcacggcgtc gcgacgaccg
    18121 gcataaccgg cataagcgtc ccaacgcacc gcgagttcgg gaagaacggt ggcacccatc
    18181 tggaaaaggt gtgccatgtg atgtccacgg gtattgcctc gatagccgcg gatgcgaaaa
    18241 ctgcccacct gctgatccgc ataaggttcc agcttccgca cgtcttcttt ggccacggca
    18301 ccgcgcatgg attcgagttt tcgatacgcc ggaaccgcca gggcagaatc cttcacttcc
    18361 ccccgctttt ccgcaaccga caaaagatgc tctgccgtgg cgcgactgcc cgcatatcca
    18421 ggcggatcaa tctccgaata cgtcaccgaa aacaacaact ctccagaagc tgccgcttcc
    18481 gccgcttcat cgaacgaggc caaccgtcgc tccaccagcg ttcccggacg gccttgctca
    18541 tatcccgtgt ggattccgtc aaggatcaag agcgcatcga gcggatcctc acctcgtcgt
    18601 tgctgtaaaa tggtcgaaat agctccatac cccgcgctcc acgcggataa ggcaacacgt
    18661 cgaagacgag cgttttccag accgcgacgc tgtactgcgg attggatggc cgccagcaag
    18721 ttttcatacg taccgggcgt ggcataagca tcctgatacg ggccggagcc gacaccgagg
    18781 ttgaccaccg cgatcgcagc gttgagtttg gaaacagccg cgctttccac caccactttt
    18841 acgtttccgt gaaagtgaat caaaaggtcg taggaaccat cgctcgaacg aaaagactcg
    18901 ggcgtataca gcacaccacc gggaatggaa gcgtatccgg cgtgctcgag agctgcatcg
    18961 gcgcgacgct gacggaacag ggaattgagt tgtgctgagt cgacctgtac catggtactc
    19021 gacgcgagct gcggcagatc gtgaacccag ctccccaagg tcatggctgg ttcctgccgt
    19081 ggcactttca cccaagtcac caaaagagca atgacgatac cgagcagtac cgccccgcac
    19141 atcgcgcttt tcccactcga acagggaacg gtttgggcga tcggttcgct cggagtagga
    19201 gagacggcaa caggcggtgg cgtgacgcta taggattcat cccaaccaaa gggctcgtcc
    19261 gcttcgtcta caccgcaaag agattcccga gacgaccggg ttttcacgtt gcggggaagg
    19321 tgcttcatga aacgatgcat ccaacacttg gtcgcttcgg gccaagttct tatgtcccca
    19381 atgatcggta tggccaattc gtcttgtcac gacggcaccg ctcgatggta tctgcctcgg
    19441 tcctccgagt attccacgga atgcttcgct ttacgctgcg gggcaatcag gctagagcca
    19501 ggccatgtgg cgcttttccc ccatcgcgat tgctttgccc ttcatgctca ccgtggggtg
    19561 cgcgggctct cagcagcaac ccgcggctcc cgagggattc aaagtttgct cgtattgctg
    19621 cacgaccgcg aataccgtct gccgctgcga ggtgatcgaa gccgtcgaat gccctgctcc
    19681 ggaccagacc gctacgctcc cggcgtgtgc cgaatgtcga agctgcacga aacgattgga
    19741 gtccggtgaa agctgccaac cgcaatgacg gtgatagggc gcatcgatcc accatgaaac
    19801 ccaaaaccct tgccattctc atcgctgctt tggttgcggc agtcatcgcg gcccttgtgg
    19861 tatgggatat cgtcgtcgtc accgatcaag aacggctcga agcatttgcg gatttggtca
    19921 cttcggaagt gagtccggaa aatatcgagg cggccatggc ttacgtagac ccggccgtgc
    19981 agcccatcgt tgttgaagtg cgacaccaaa cacttcggtt cgacaaggac aatcgcgatg
    20041 acttctcgct catggcccgc ggccgcttgc gcccgtacca tggcatcaaa cagcacgtga
    20101 tgcgaaaaag cgtcgacgtt cgggattctc aggcttccat caataccgag accttttcgc
    20161 ggcaaggaag ggtttcggtc gattgggaac tgcgcaaacg cggtgaggat tggtgggtca
    20221 gccgcgtggc ccttcgatag gatcagggag cgggggcagg cgtttttctc gcatcttccg
    20281 ccgccaaggc ctcttccgaa gggggttccg gacgaaccgc ctggcgttcc caggtccatc
    20341 ggtcgagcgc ttcttcactg ggggtgagca agcccatgac cgaggtcatg acaagaaaaa
    20401 aagtagccgt aacagccacg aaccagcatg gaaatagacc ttctacccgc agccgctggt
    20461 aatagtccca aaccgaaacc gggctatgag gatggatgaa cactttggtg agccataccc
    20521 cggtgatgaa gtacacaagc agcaagtagt tgcgccgcag cctcaagcgc gtggccatat
    20581 accaactcat ggtaaagtga gggcgtcgaa ggtcgaaagc gagttgcctt ctccaccctg
    20641 gttccgccag tcgagccgaa ggccaaagca tgttggcgta gtagttgtgg tcgaacaggg
    20701 cgagtcgcca cttgctatga tgcaaacgct ggtagcgtcg tgcttcgatg accagacaga
    20761 tcgaaacgat ggccaaaccc aggagcatta cgtaatgggg gacgctggtg gtcccaagca
    20821 cgaaggtaga catgccgaag gtcagaagga tggcccagtt gctcgtggta tcgagtcgtt
    20881 gtcgccatac ggtcatacgg ttcatttccg cgcgataaag atgggcgatg gccgtatgaa
    20941 agacagcggt ttcttgatgg caatcggcgg gggccgagtt ggggtcgaag ctggtgggca
    21001 tggaagtcgt ttgcctgctc taatccgatc gttttgaaac gcaagaaggg caaagaacgg
    21061 cctatcgcgt ggatagcccc gcgaatggtc tggctcgaag accgccattt tcgcaaggat
    21121 gggaagcgac tcagggggcg gcgtgggcgc taacctatcg aatctcatcg gtttgccagt
    21181 cgcgcggggt atcggagcga acgaacccgg gttgcctatc gtgcaggctt atggaggacg
    21241 gtagactcga agcgacactc gcgcatggaa cgatgagcct tcgaatagcg ccatggattt
    21301 ggtggttcat ggtagcgcaa ggcgtgcatc ggacaccgct ttgctcccgt gagtgttgcg
    21361 ggggaaagga agcagagatg tatatttatt aggtatttca aatggttacg ttttagagat
    21421 ggctgtgagc taccgtccca ctaccacgag ggtgcctcgt tcatttcggt ggggagcgat
    21481 ggcttgccag ccatcaggta gcgtgggttg ggtgaacgtg aaaagtcgtt cggcatcgta
    21541 gggggaaagg ttggggcatc ctgcgctggt gggaataccg aaacgttcgt gccaataggc
    21601 gccgtgcaat gcgtagggtt gctcgaagta ttgaacgaac atgacctggc tgatccaaaa
    21661 cgtggtggga gcgcctgggt ctggagaaaa tgtcgcatac cgctctttcc aattgatggg
    21721 aaacgaacca aggggtgtcg tgtagttgcg gacatctcct ttgcctcggt gaacgccgcc
    21781 tcgtccggac gcatgcaagg tggcgaaaac cggacgcaat ccctcgtaag cgaccagcgt
    21841 tcgctccagc gtggatacgt aaatccaagg ctcgtcgtca gcgattcctt caggtcggtt
    21901 gtcgaccaga ttcacttcac agacctggtg agcgagtacc caatgagaat catcacggag
    21961 ttttcggtac tcccgacctc ggattcgctc cgtctgtggt gtcaaccgca cgaggtggcg
    22021 tgcggcgaaa gactgccccg tcgcaaccat ttttccgtct tgcagggcaa agcgggcggt
    22081 tggggaagca caggtccatc cgaagtgcgg cagcagttcc cttgtgatcg taacgccgcg
    22141 aaaagatgtg ggtttataga tgagcacgcg gtcggcgggc acggcggaaa ggtcatcggc
    22201 caacaagtac cggcgtcctt gatattcgaa tgctcttgtg aaggcgagca tggagccatg
    22261 aggaagtttt cgacgcacga atccgtgggt tgcgtttggg cttgcctttc cgtcgagaaa
    22321 aaagtcaggg acgggtttga tgtccgacgc gggatgaggg tctacgag
//
LOCUS       DAOSOR010000022        22271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_2335_length_22271_cov_4.828052, whole genome shotgun sequence.
ACCESSION   DAOSOR010000022 DAOSOR010000000
VERSION     DAOSOR010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 22271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22271
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2335_length_22271_cov_4.828052"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..1170
                     /locus_tag="PK710_02225"
     CDS             43..1170
                     /locus_tag="PK710_02225"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOT08552.1"
                     /translation="MSPPEIAQTLSQQLLSSLVQQGFDPSLFDTLAASIGSDPDARNR
                     LTTGVTPPAADDILDLPPAGSDEYHQLSRLGLEALACGQLAFVVLAGGMATRMGGVVK
                     GLVHALNHQSFLDLRLIENLVWSKRAGRDIPLWLMTSHATDSALREALGSHLEGDRLA
                     TFRQNLSLRLNPDGSLFHASDGSPSVYAPGHGDLPDALRRAGLLERFVQRGGKVVWVA
                     NIDNLGAGIDPVILGWHLRHGAPVSVEVVDKIGTDRGGIPVRLDDRPVILEEFRLPRD
                     FDPSQVRVFNTNTFLIDARLLATSRFDFTWVQVTKQVDGAKAIQFERLLGEITTALDT
                     RFLRVPRQGTTSRFLPVKDNDELAFRRDEIQTVLKARGVLP"
     gene            1223..3757
                     /gene="sppA"
                     /locus_tag="PK710_02230"
     CDS             1223..3757
                     /gene="sppA"
                     /locus_tag="PK710_02230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOT08553.1"
                     /translation="MRVRPSLSSLITSAAALTILISSSIHAQPRALSVPSPGRSAASS
                     DDGHALVLNPANISHIPGAELRWTWVQPGQDPLALPRGHAFSLAGAFPFGLGTGLRLD
                     FLRPHEQHARASTFLDEPYTWLTWGLGFGTETSALGMSIRHLYASEPGVGGPTTISLG
                     ASIRTSPVLGLGAVIHDINAPTAADGRGFADRSYTLAAAFRPFGKRLLELGLEGRYYE
                     GFRFPARNTTDASFPIDQGWVPRATLGFDVPYVGRLLADVTVPRESAWMATTSLDINL
                     EHSTVTGGAIFGNAIGGKDGAGFITGLALTSWREPGIPDPSYALKIRIEQTPSNRGHV
                     DFLRQLWRISKNPEIAAVVLHLKTEPASTLAHAYEIDDAVRLIRARGKKVVCHLEDAG
                     GRSLLACSSADRIVVNPAGGLRFAGLRNERLFLAGLLQKIGVRAQFVRIGDHKSAPEQ
                     FTNTEPSPIAKADSIEHLATLTREMTQVIAHGRHSDPSTIQRAIDAGPHTAREALAHH
                     LVDGYAYDDELRTVVSEVVGRGVDLRDDLPNYAPERFGRRPSVAIVYVEGNIVDGRSM
                     DIPLLGMQIAGSYTIAESLKKARENPDIRAIVLRIVSPGGSSMAADVMWREVALTAKI
                     KPVIVSMGGVAASGGYYIAAPGSKIFATPFTVTGSIGIFYGKADVAGLLEKLGVNVDT
                     IKTSPRADAESIFRPFTDEEVEELGLKVKQFYDVFIDRVAKGRKLDPERVDRVARGRV
                     WLGRKAVDHKLVDDIGGIRQALNAALAVSNLPDDTPIIELPPPQFSLLNLAASMVSTD
                     SLEPPEAKWLRHHVPGEIGKILQAVAPFVVYDPFQPLALTEMTEIP"
     gene            3754..4698
                     /locus_tag="PK710_02235"
     CDS             3754..4698
                     /locus_tag="PK710_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08554.1"
                     /translation="MTSTPLLSLHHARIDRDGSLLCENIDLEIDGPRVVVAGPGADAV
                     LEAICMTANVSKGHVRISGYDVSTRSHVGRVGIAPSDLPLPQRMSVEQYLILYYRTLG
                     FSKREANHATMTSLSDLGLTHLASRQSNSLTLSERKAVTIAAAMLPNAPCLVAHSPLR
                     GIEKPESHGILSVLGQVANRRQVLVSTSRWDAEGVDAELMAGATHIAFLSRHATLWSG
                     PTSHLFSEGKQAALLVSGTDDAFVRALAQQGFDPTGKLPRIVVRIDDPNGTSRLLSIA
                     NEYDVAIIQMIPLWQPPRAPSSSEPEVGPNDQEPPSHD"
     gene            4691..5356
                     /locus_tag="PK710_02240"
     CDS             4691..5356
                     /locus_tag="PK710_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08555.1"
                     /translation="MTDQDLHSSAKALSRSPTFPPGRLTLLTALGAASGSLPLPVLPG
                     RLVTNIRGAIAFDVARQHGLSLTAEARRALARASSVDPTRAQLIGIVSWTARRLLRRV
                     SPLWVLAPALSAIETFALGHLMNRYLEEARTHRGIRIETDEARHIRRIVEQSLLKTLS
                     PEIVVSQEPRADYPHGDERDDLTRLIDWTLLTTASFPSYLLRRLDASFEAVIRESKNA
                     PSE"
     gene            5353..5781
                     /locus_tag="PK710_02245"
     CDS             5353..5781
                     /locus_tag="PK710_02245"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HOT08556.1"
                     /translation="MNNLLNTQLVVASVIIQEARVLVTQRAQGSHLAGKWEFPGGKVE
                     QDEDPRDALQRELREELGIDINVGDVLEVVFHRYSSKNILLLFFFASIRPHSPPPQPL
                     EVAAFAWRTEHELAEQDFPPADTAVLSRVRSLLRQATRED"
     gene            complement(5760..7208)
                     /locus_tag="PK710_02250"
     CDS             complement(5760..7208)
                     /locus_tag="PK710_02250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT08557.1"
                     /translation="MLFVFLVLRVSLGLAGFALAYFMARARSFRPEHRLLAALGVLVG
                     LESVWAIAESFGNHGPRFLNGEPWMTVVLVAVAATALGLFARRKRTFSFSTGSIDRDG
                     TNGSEPEAEKGLSSVPRQTRHLDADWRDVVDCLPVGIVWIAGPSLQVQMWNRYEAQRT
                     GIEARQVLGKRYLDQVAERHLDGRLVEAIRAKPQEIVTFPSVSWSVQGESGIETTIVP
                     FRSEHEQEGYLIVTFDTTERDAYFREREEVRKQAALREFASAIAHDIRTPLSSIRMSV
                     QILRSKAVLSPEDMEYFDLTLEQISRLGTDIDELLVFTKPALLEVKPVLLRELVEDAR
                     EQVLSAMQRDVAIVIDVPCELKVPVDEEQVHRMLLDLLRNAVSASPSGSRVWIAAHQE
                     RDEVTILVRDEGHGIESCDLERIWEPHFKKQADGTGLGLALVRKIVTAHGGRVTVHSE
                     CGRGSTFTVVLPASRATEVVVPIEECQSSRVA"
     gene            complement(7220..8665)
                     /locus_tag="PK710_02255"
     CDS             complement(7220..8665)
                     /locus_tag="PK710_02255"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOT08558.1"
                     /translation="MSETDKVQEGMNTILIVDDDRAIRRTLEKYLGDAGYVVVTASDG
                     ESAWERLCEGTADVCLLDLGMPGVDGMEVLRRMHQAWPLDTTPPGVIIISARDDMHTT
                     VQAVKLGAYDYLVKPLDANRIKLTVQRALEQRRTSLTLAHMVAEEREDRGVDSIVGKS
                     EAIREVYKRIGAVATSRATVLIRGESGTGKELVARAIHHASGASNAPFIAVNCTAFPP
                     DLLESELFGHVRGAFTGAVADKTGRFQLAGQGTLFLDEIGELRPDLQAKLLRVLQERV
                     FERVGDSRPIKLAARVVAATHRNLEEMMGAGSFREDLYYRLRVFEILLPSLRDRREDI
                     PMLVDYLLARIARETHKQVRYVSSEAMDALLQYRWPGNVRELENALTRGVVLCKGDVL
                     TADVLPIVPDDDPDDGMDVEDRKAFADETLEPLLTLREVEKRHIERVLTHTGWHKRRA
                     CAILQITRPTLDRKIRDFHLERPPADRVRAS"
     gene            complement(8727..9497)
                     /locus_tag="PK710_02260"
     CDS             complement(8727..9497)
                     /locus_tag="PK710_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HOT08559.1"
                     /translation="MPKIKFLVEHREVDIASGRTIREVALEAGIYLNREFFSGFNCGG
                     RGLCGTCKVWVRESSRNATSPPNFRERFHGMGDGRRLACQTRVFEDIEVTSMPGGQDR
                     LEPSRIIDPAPQRPADPEPPAKAETSPAEKKAKDTKPKDDKPKGDKPKDDKPKDDKPK
                     DDKPKGDEPKDDKPKDDKPKGDEPKDDKPKGDEPKGGEPKDDKPKGDEPKDDKPKGDE
                     PKDDKSKDDKPKDDKPKDDKPKDDKPKDDKPKDDNSEE"
     gene            9704..10981
                     /gene="glyA"
                     /locus_tag="PK710_02265"
     CDS             9704..10981
                     /gene="glyA"
                     /locus_tag="PK710_02265"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOT08560.1"
                     /translation="MNTAKQITWHEPPLAETDPEIFALIEREEAYQRDTVRLIPSENY
                     ASRGVLEAAGSVLSNKYSEGYPFKRYYEGQAQIDAIEELACKRVAELFGAEHVNVQPY
                     SGSPANFAVYAAFASPGDTVMGMMLPAGGHLTHGWGVSVTGSFFKAVQYGVRENDHLI
                     DYDQARNLAKEHRPKILFCGATAYPRIIDFQAFRSIADEVGAILVADIAHISGLVAAG
                     VHPSPVGLADVVTSTTHKTFRGPRGGMIMCKKAHRKAINKAVFPGLQGGPHNQTTAAI
                     AVAAREAATPEFKAYAQKIVENAKALAEELNARGFRLVTGGTDNHLMVVDLTNKGVPG
                     KTAAKALDRAGIVVNSNTVPFDPRTPFDPSGIRIGTPAITSRGMGMGEAKRIGAWIDD
                     VVTHHEDEAVLTRIAAEVKEMCSQFPAPGLPIG"
     gene            complement(11092..11760)
                     /locus_tag="PK710_02270"
     CDS             complement(11092..11760)
                     /locus_tag="PK710_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08561.1"
                     /translation="MPLNQPVDLPSLSDARALARKEVEDPELQALPGPPKRQRTLAAV
                     LMLLTMVASCAMGWSLRSEVQYAVSSSFPIAIGELASLEPSSLTPNQYVVARGLLGTA
                     GAVRYARPFEGDSFRLQPVAGTARVWVEIRVPEGMEGPRFVPPSEFTGRLVPLSKAGL
                     RLSGVTRSVVQQTGQTIAPDAWVLVDGASPRASRWAIALVVLFAFFAVWNGVSIIRIL
                     RPIR"
     gene            11901..12434
                     /locus_tag="PK710_02275"
     CDS             11901..12434
                     /locus_tag="PK710_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger Ran-binding domain-containing
                     protein"
                     /protein_id="HOT08562.1"
                     /translation="MAKSKTVLCPSCGFKNSSTQKRCVSCGAKIEQLGPTARSREEEL
                     ERRYQQEGFSLIWMLVALVVQGVLTAAIVIGLPMVVQAFDFEGIYGMYMCVPVWFLGG
                     LLVGLISPGKTFIEPVVAAFFIAVPTVFYLVQSQTVRTMPTFMYVLIGCVGVMFTLIG
                     SYIGERIQMGPPPKLSD"
     gene            12641..14254
                     /locus_tag="PK710_02280"
     CDS             12641..14254
                     /locus_tag="PK710_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08563.1"
                     /translation="MHRLSAPPPPKITSLSPRRDAYIDLLEDSRGLRREQQQARANWL
                     STLSPERQELVFELEVLLKGAACFSNPRNHPGKPRRVPTVAQDFREPLALLHKGFKRI
                     THLARLCLGDRDKAFVFHRYLETLLPEDTARTRLFREDSSQSTPQGSLFALRRGFSNL
                     VEVGDGLLRLPRVPFRLFYATANLAQREVSQNAFFNPLSALEFRPEFDRIQSTEVVEM
                     IGRVPGEHAQRLTSLTFLSLFRMLRYLRLLEHIVPDLSDPLGTAAGSLYLVLSVLRSD
                     ARALATYLRRKSGEQLASSFERDLLMTPSSQLRDRYEELLTTGHRLIDARGAFEGVSA
                     NLRLELQRVFEHTIPSPEEGSGSTRLRDDVLTGIAELRPTLQNVIMLLARTLGATLEE
                     DVVFDDQLAKRQISERLRRHIWMFAQIIRAFMVKARHAQGAGEAWDTVSSFQFVREFV
                     AYFRAMGYPLLRASDYPRFASFLHAMNALQDADLLDPDKLDRAIIECEAFHAFLSELL
                     ERVGQREELQGIAFDKRAAAMTLRLYLRE"
     gene            complement(14497..14958)
                     /locus_tag="PK710_02285"
     CDS             complement(14497..14958)
                     /locus_tag="PK710_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08564.1"
                     /translation="MAAVKKTTKAKTKTAKKAPKKVAKKAAKKAPKKAAKKVAKKAVK
                     KAAKKAAKKVAKKVVKKTAKKVAKKAPKKVAKKVAKKTVKKAAKKAPKKVAKKVGKKA
                     AKKVAKKAPKKAVKKASPRSVKREPKKTIRGRKASSKELIAPVASYNDGEE"
     gene            15308..16129
                     /locus_tag="PK710_02290"
     CDS             15308..16129
                     /locus_tag="PK710_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT08565.1"
                     /translation="MAWYRSVGLSFLTTLLLAFVLGGCGHSEEEWQAKLKEVDGLKAQ
                     LQAERQAHQKTQDDYNAAKAEIDELKDRLKQAGVDVQQLNASLAEQRKALEEFKRRAE
                     QLEAIRKRFELLRSKLDSLVKLGLSVSVRNNRMVISMPGDVLFDSGKVDLKKDGEAIL
                     RQVAEVIRGDAQLNARTFQVAGHTDNRPLGGGPYKDNWGLSLMRARQVLIFLVEPLDK
                     EGGGLNPLRWSAAGYGDTDPIAPNTTPDDMRKNRRVELIVLPNVEEMLDLQSLTR"
     gene            16185..17111
                     /locus_tag="PK710_02295"
     CDS             16185..17111
                     /locus_tag="PK710_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HOT08566.1"
                     /translation="MKVATIDIGTNSVLLLIAEIHNHQLVALQEESTITRLGQDVDHS
                     RRLHPQAIERTISCLHRYAEAIRGHGVSQMDAVSTSATRDAQGVDDFLDSAERVLGVR
                     PRIISGDTEARLSFQGALSGLDVQGPVMVYDVGGGSTEIVLGHVDQGRRWHIEASHSL
                     DIGCVRLSERHIRSDPPAEVELTNLRTEAALLFGPMSLDAPARTLIGIGGTVTTLAAV
                     HLGLASYDPTKIHGALLTTTQISRLASRLAQLPLAERQILPGMERKRADVIIAGAIIV
                     EQILLSTKSPETRVSNRGLRWGLALDLIAPHV"
     gene            17355..17861
                     /locus_tag="PK710_02300"
     CDS             17355..17861
                     /locus_tag="PK710_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOT08567.1"
                     /translation="MQARSEVKFKVGDKAVYPAQGVAEVISIEEKDIAGSRQRFYVLR
                     IMDTDRKIMVPVNNASAVGLRQVISEQEIREIFDILRERTIAFDNQTWNRRYRGFMDK
                     IKTGSIYDVAEVLRDLYRLKTDKQLSFGERRMLDTARTLIVKEIAISRGQTEEQVRTE
                     IEAIFLAN"
     gene            complement(17927..18436)
                     /locus_tag="PK710_02305"
     CDS             complement(17927..18436)
                     /locus_tag="PK710_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08568.1"
                     /translation="MKGEAGQKLGIRIRKEVRLSRRQALAWFAGMAVGCRRRDVDETP
                     EGVVQEWLERMARVHGDPRDAQQAYELLSSETKSNLQERARRASAATGRKMEPESMLA
                     PSRFSLRFTPRRMRSRVAGDRALVEVVGSDPELERAEVPCVLEQGKWRVDLVLPPLPA
                     VEKRPDAGG"
     gene            18568..19563
                     /locus_tag="PK710_02310"
     CDS             18568..19563
                     /locus_tag="PK710_02310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOT08569.1"
                     /translation="MKPRGPRQAPQNTNPLPVPHASDLRLFHGDNLDVLAALSPQFQK
                     KFTLVYLDPPFFTMRQHERVIRKTNPHGPPIRLFTPAFDDRWQDFPDYLGSLRARLEA
                     LAPLLAPHGSLVLHVDPRTSHYLKIIGDEIFGPECFASEIIWRYRRWPTRTANFQRMH
                     DVLLRWVGDPRQPPRFVQQYEPLAASTLKTWGTGKQKAVFDDGGRRRRSSTESQQSPG
                     VPLGDVWDIPILAPVAKQRTGYPTQKPEALLERLIRSCSFPGDWILDPYVGSGTTLAV
                     AAALGRHAVGIDASAEAYQVSQQRLRQAGHPISTFSTTSNSNHTPIGSRSSAQVS"
     gene            19597..20619
                     /locus_tag="PK710_02315"
     CDS             19597..20619
                     /locus_tag="PK710_02315"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOT08570.1"
                     /translation="MKRAGILVPCIGLGLSLIALMLLASPNHSRASKSPDGSTRVVAT
                     VGQATITAAELERRLSAVPAFQLRVYGSTPEEVKRNYLSRVLVPELLFAQGAMDRGKH
                     EAAEIRSRQRDILKTALLDQLRQESVQQEAVTPEQIAAYYQANRERYQTPARVSVWRI
                     LVADHELAKNLIEQAQKNPTPKAWTELSQKYSLDKSTNLRGGNLGFLAADGTSADGKT
                     RVSEPIAKAAFAVRDGEIVKEPVAEGAGFAVIWRRGSMPAVHRTVADEAKAIEKILLR
                     DRFRAKQDALIERLRRDHLRLLMPDGPELIAVSGGGQIEQERKPGRIVRRPGRTEPFA
                     TPRGLR"
     gene            complement(20521..21135)
                     /locus_tag="PK710_02320"
     CDS             complement(20521..21135)
                     /locus_tag="PK710_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08571.1"
                     /translation="MNRRLPVLLPLALSTVALMLLFSFPCWAGPYLNTSTMLLVESDH
                     AGRWVRTNLGDKELAKNAQKMAQARLDVASTMVVPPEVREAHPHLLLALAAMERAMQA
                     AIDGRISDFIRQIQTSSGEAKTFQAVLQAAGFTLPNYHRAAAPSAAQPRHAEATEKLN
                     PPAALQAPRRFRSPKTARRGERLRAPRTANDTTGFSLLLNLPAS"
     gene            complement(21132..21824)
                     /gene="rnc"
                     /locus_tag="PK710_02325"
     CDS             complement(21132..21824)
                     /gene="rnc"
                     /locus_tag="PK710_02325"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOT08572.1"
                     /translation="MMDRTALLQLLDIEGPVRRLDEAMTHPSFVNEHPGQSDYQRLEF
                     LGDAVLGLCVTELLLSHAPTAKEGQLTRMRASLVNTQALADFAHSIHLAQWVNLGKGA
                     SLSGDALQPKVLADVVEALVGAVYLEHGLDGARKLTRRIVDEPMTRRGKIAQRDPKSE
                     LQERVQRGGGATPVYHVLSVSGSDHDLRFDVEVRVEDIVLGRGQGRSKKSAEQAAARM
                     ALINHRSNEKTS"
     gene            22055..>22271
                     /locus_tag="PK710_02330"
     CDS             22055..>22271
                     /locus_tag="PK710_02330"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOT08573.1"
                     /translation="MQKPPIVARTNEHFRLSRGFTLLELMTVVILVGIIAAIAIPTAS
                     RLMRENRSSTAAQRIALMYQVARARAMG"
BASE COUNT         4746 a         7001 c         5748 g         4776 t
ORIGIN      
        1 cacgctccac caagacccgt tcccaaaacc aacgaggcct ggatgtcgcc ccctgagatc
       61 gcccaaacgc tgtcacaaca actcctttct tccctcgtgc agcaaggttt cgacccctcc
      121 ttattcgata ccctcgcggc atccatcggc tccgatcccg acgctcgtaa tcggctcacg
      181 acgggcgtca caccgcctgc tgccgacgac atccttgatt tgcctccggc gggaagcgat
      241 gaataccatc agctctcacg acttggcttg gaggccctcg cctgcggtca actcgcgttc
      301 gtcgtgctgg ctgggggaat ggccaccaga atgggcggtg tcgtcaaggg actcgtccac
      361 gctctcaacc atcaaagctt tttggacctt cgcctgatcg aaaacctcgt ctggtccaag
      421 cgcgcgggca gggatatccc cctttggctg atgaccagtc acgccactga cagcgccttg
      481 cgcgaagccc ttggctcgca cctcgagggc gaccgccttg caaccttccg ccagaatctt
      541 tcgttgcggc tcaatccgga tggctccctg tttcacgcct ccgacggttc tccgagtgtc
      601 tatgcccccg gccacggtga cttgcccgat gcgcttcgtc gagccggtct tctcgaacga
      661 ttcgtacaac ggggcggtaa ggtcgtttgg gtcgcaaaca tcgataattt gggcgcgggc
      721 atcgatccgg tcatcctcgg atggcatctt cgtcatggcg ctcccgtctc cgtggaagtc
      781 gttgataaaa tcggaaccga cagaggcggt atccccgtgc gccttgacga tcgtccggtc
      841 atcctcgaag aatttcggct tccccgtgac ttcgacccat cccaggttcg agtcttcaac
      901 accaacacgt tcctcatcga tgctcgcctg ctggcaacgt ctcgattcga cttcacctgg
      961 gtgcaagtca ccaaacaggt agatggcgcc aaagcaatcc aattcgaacg gctccttggc
     1021 gaaatcacaa ccgccctcga cacgagattt ctgcgcgtgc cgcgacaagg caccacctct
     1081 cgattccttc ccgtcaaaga caacgatgag ttggctttcc gacgcgacga aatccaaacg
     1141 gtcttaaaag ctcgcggtgt actgccatga tgctcgatcc aacccgcggc ataactgccg
     1201 ctatttgacg tgaggaaaaa ccatgcgcgt tcgtccgagc ctttcgagcc taatcacttc
     1261 cgccgcggcg ctcacgatcc ttatctcctc gtcgatccac gcacaaccac gcgctctttc
     1321 cgttccatct cctggtcgtt ccgcggcctc cagtgacgat ggccatgctc tcgtcctcaa
     1381 tcccgcgaat atcagtcata tcccaggagc cgaattgcga tggacctggg tgcaacctgg
     1441 acaggaccct ctcgctttgc ctcgcggcca tgcctttagc ctcgcaggag cattcccttt
     1501 cggtttgggc acaggtcttc gcctcgactt ccttcgaccc cacgaacagc acgcccgtgc
     1561 atcaaccttc ctcgacgaac cgtacacatg gctaacgtgg ggacttggat ttggaaccga
     1621 aacttccgcc ctcggtatgt cgatccgaca tctttatgca tcggaaccag gggtcggtgg
     1681 gcctacgacc atctctctgg gcgcttccat tcgcacctct cccgtccttg gcttgggagc
     1741 ggtcattcat gacatcaacg cgcccaccgc cgctgacgga cgcgggttcg ctgaccgaag
     1801 ctacaccctc gccgccgctt ttcgaccttt cggcaaacgc ttgctcgaac tagggctcga
     1861 aggacgctac tatgaaggct ttcgctttcc cgcgcgaaat actaccgatg cgtcgttccc
     1921 catcgaccag ggttgggtac ctcgcgctac gttgggattc gatgtcccct atgtcggaag
     1981 gcttcttgcc gatgtcaccg tccccaggga atcggcgtgg atggcaacga caagcctcga
     2041 catcaatctg gaacactcca cggtcacggg aggtgccata ttcggtaacg cgatcggtgg
     2101 aaaagatggc gcgggattca tcacaggtct cgccctgacg agttggcgcg aacctgggat
     2161 tcccgaccca tcctacgccc tcaaaattcg gatcgagcaa acaccctcga atcgaggcca
     2221 tgtggacttt cttcgacagc tctggcgcat ctcgaaaaac cccgagatcg ctgccgtcgt
     2281 gctgcatctg aaaaccgagc ccgcatcgac cctcgctcac gcttatgaaa tcgatgatgc
     2341 cgtccgcctg attcgcgctc gtggcaaaaa ggttgtctgt catcttgaag atgcaggagg
     2401 gcgctcgttg ctcgcttgct cgagcgcgga tcgtatcgtg gtcaaccccg ctggcggcct
     2461 gcgtttcgcc ggcctccgaa atgaacggct cttcctcgct ggactactgc aaaaaattgg
     2521 cgttcgcgcg caattcgtcc gcatcggcga ccataaatcg gccccagaac agttcaccaa
     2581 caccgaacct tcccccattg ccaaagccga cagcatcgaa cacctcgcga cactcacgcg
     2641 tgagatgacc caagtgatcg cacacggacg acactccgat ccttcgacca tccaacgcgc
     2701 catcgatgcg ggacctcata cggcacgaga agcgttggct catcatctcg tcgatggata
     2761 tgcctacgat gacgaactga gaaccgtggt gagcgaagtc gtgggccgtg gtgtggatct
     2821 gcgagatgac ctgcccaact acgcgccaga gcggtttgga cgacgaccat cggtcgcgat
     2881 cgtctatgtc gaaggcaata tcgtcgatgg ccgaagcatg gacatcccct tgctcggcat
     2941 gcaaatcgcc ggctcctaca ccatcgcgga atcccttaaa aaagcccgag aaaaccctga
     3001 tattcgcgcc attgtcttgc gtatcgtgag ccccggaggc tcgtccatgg ccgccgacgt
     3061 gatgtggcgc gaagtagccc tcaccgccaa aatcaaacct gtcatcgtgt ccatgggagg
     3121 agttgctgcc agtggcggct actacattgc cgcccccggt tccaaaatct tcgcgacacc
     3181 tttcaccgta accggttcca tcggcatctt ctacggcaaa gcggatgtgg caggactgct
     3241 cgaaaaactc ggcgtcaacg tcgataccat caaaacctca ccccgcgccg acgccgaatc
     3301 catctttcga cccttcacgg atgaagaagt cgaagaactc ggcctcaagg tcaaacagtt
     3361 ttatgacgtc ttcattgacc gcgtcgccaa aggaagaaaa ctcgaccccg aacgcgtcga
     3421 tcgcgtcgcc cgcggacgcg tgtggctcgg gcgaaaagcc gtggaccata agctcgtcga
     3481 cgatattggt ggaattcgac aagccttgaa cgccgctctc gccgtgtcga atttgcccga
     3541 tgacacgccc atcatcgaat taccaccccc ccaattctca ttgctcaatc ttgccgcaag
     3601 catggtttcc acagactcgc tcgaacctcc tgaagcgaaa tggctccgcc atcacgtccc
     3661 cggtgaaatc ggcaaaatcc tgcaagccgt cgcgcctttc gtcgtctatg acccgttcca
     3721 acccctcgcg ctcaccgaaa tgacggaaat tccgtgactt cgacccctct gctttcactc
     3781 caccacgctc gcatcgatcg tgacggctct ttgttatgtg aaaacatcga cctcgaaatc
     3841 gacggacccc gcgtcgttgt cgccggaccc ggagcagacg ccgtcctaga ggcgatttgc
     3901 atgaccgcca acgtctcgaa aggccacgta cgaatttccg gatacgacgt ttctactcgt
     3961 tcccatgtcg gacgcgtggg aatcgccccc tcggatctac cgcttcccca acgcatgtcc
     4021 gtcgaacaat acctcatcct gtactatcga actctcggct tttcgaaacg ggaagcaaat
     4081 cacgcgacca tgacgagctt atcggatctc ggcctcacgc acctcgcaag ccgacaatcc
     4141 aattccttga cgttatcgga acggaaagcg gtcaccatcg cggctgccat gctccccaat
     4201 gccccttgtc tcgtggctca ctcgccgttg cgtggcatcg aaaaaccaga aagccatggg
     4261 atactatccg ttttgggaca ggtggcaaac cgacgccaag tgctcgtgag cacaagtcgt
     4321 tgggacgccg aaggcgtgga tgccgaactt atggccggcg ccacgcatat cgctttctta
     4381 tctcggcacg ctaccctttg gtcaggtccg accagccatc tattctccga aggaaaacaa
     4441 gccgccttgc tcgtaagcgg caccgacgat gccttcgtac gggcattggc tcaacaaggc
     4501 ttcgatccaa caggaaagct tccccgaatc gtcgtgcgaa tcgacgatcc gaacggcaca
     4561 tctcgcttgt tgagcatcgc caacgaatac gatgttgcca tcatccaaat gatcccactt
     4621 tggcaacccc caagggcacc atcatcatcg gaacccgagg tgggacccaa tgaccaagag
     4681 ccaccctccc atgactgatc aggatttgca ttcatccgcc aaagcgttga gccgctctcc
     4741 cacgtttcca cctggacgcc tgactttgtt gacggccctg ggcgctgctt ccggctcctt
     4801 gcccctgccc gtgctgccag gacgactcgt taccaacatt cggggagcta tcgcgttcga
     4861 tgtagctcgc caacatgggc tcagcttgac tgcggaagct cgccgcgctc tggccagagc
     4921 aagctccgtc gacccaactc gcgctcagct catcggtatt gtttcatgga ccgcccggcg
     4981 attgcttcgg cgcgtcagcc cattgtgggt tctggccccc gctctatccg ccatcgaaac
     5041 tttcgccctc ggtcacctga tgaatcgata cctcgaagaa gctcgaaccc atcgcggtat
     5101 ccgaatcgaa accgatgaag cacgccatat ccgacgcatc gtcgaacagt ctttgctcaa
     5161 aacgctttcg cccgaaatcg tggtctccca agaaccaagg gcggactatc cacacggcga
     5221 cgaacgagat gatctcacgc ggctcatcga ttggacgctg ctcaccaccg cttcgttccc
     5281 ttcgtatctc cttcgacgac tcgacgcatc tttcgaggct gtgatccgtg agtcgaaaaa
     5341 cgctcccagc gaatgaacaa tctcttgaat acacaactgg tcgttgcttc ggtcatcatt
     5401 caggaagctc gcgtcctcgt gacacaacga gcacagggtt cccatcttgc gggtaagtgg
     5461 gaattcccgg gtggaaaagt agagcaagac gaagaccctc gtgacgctct gcaacgcgaa
     5521 ttgcgcgaag aactcggcat agacatcaac gtcggtgacg tcttggaagt ggtcttccac
     5581 cgatactcgt caaaaaatat cttgcttctg tttttcttcg cttcgatacg cccacactcg
     5641 cccccgccac aacctctcga ggtcgctgca ttcgcttggc gtaccgagca tgaactcgcc
     5701 gaacaggact tccctcccgc ggataccgct gtcctttccc gcgtgcgctc gctacttcgt
     5761 caggcaacgc gcgaggattg acactcctcg atcggaacca cgacctccgt ggcccgactt
     5821 gccggcaaca ccaccgtaaa tgtcgaccct cttccacact cgctatgaac cgtgactcgt
     5881 ccaccgtgcg cggtgacgat cttgcgcacg agcgccagcc ccaaacccgt gccatccgct
     5941 tgtttcttga aatgcggctc ccaaatacgc tccaaatcgc atgactcgat gccatgcccc
     6001 tcatcccgaa ccaaaatggt cacttcatct cgttcttgat gcgccgcaat ccacacgcgg
     6061 ctgccagaag gacttgcaga aactgcattt cgcaataaat ccaaaagcat acgatgcacc
     6121 tgctcctcat cgacaggcac cttcaactca caggggacat cgatgacgat cgctacatcc
     6181 cgctgcatcg ccgatagcac ctgttctcgt gcatcctcca ccaactctcg gagcagtacc
     6241 ggttttactt cgagcaacgc cggcttcgtg aacaccaaca attcgtcgat atccgtgccc
     6301 aaacgcgaga tctgttccaa ggtcaaatcg aagtactcca tgtcttcggg cgataaaacg
     6361 gctttcgagc gaagaatctg tacgctcatc cgaatcgaag acaacggcgt tcgaatgtcg
     6421 tgcgcaatcg cggatgcaaa ctcccgtaac gcagcttgct ttcgcacttc ttccctctcc
     6481 cgaaaatagg catcgcgctc cgtcgtgtcg aaggtcacga tcaaataccc ctcttgttcg
     6541 tgttcggacc gaaagggaac aatcgtcgtc tctattcccg actccccttg caccgaccaa
     6601 gaaacgcttg gaaacgtcac gatctcctgg ggcttagcgc gaatggcttc caccaacctg
     6661 ccgtcgagat gccgctcggc aacttgatcc aagtaccgct tgcccaagac ctgtcgcgcc
     6721 tcgatacccg ttcgctgagc ttcgtaacga ttccacatct gtacctgcaa cgaagggcca
     6781 gcaatccaca cgattcccac cggaagacag tccacgacat ctctccaatc cgcatcgaga
     6841 tgtcgcgttt gtcgtggtac cgacgataat cccttctcgg cttccggctc cgaaccgttc
     6901 gttccatcac gatcgattga gccagtcgaa aacgaaaagg tacgcttgcg tcgtgcgaaa
     6961 agccccaaag ccgtcgccgc aacagcaaca agcacaacgg tcatccacgg ttcgccgttg
     7021 aggaaccgtg gcccatggtt tccaaacgat tcggctatcg cccagactga ttcgagacca
     7081 acaagcacac ccaaggctgc gagcaaacgg tgttcgggac ggaacgatcg cgcgcgcgcc
     7141 atgaaatagg caagcgcaaa acctgccaag cccaacgaca cccggagcac gagaaacaca
     7201 aatagcattc gttcaatgtc tacgatgctc gaacccggtc cgcgggtggc cgctcgagat
     7261 gaaaatctcg gatcttgcga tcgagcgtcg gcctggtgat ctgcaaaatc gcgcacgctc
     7321 ttcgcttatg ccatcccgta tgggtcaaca cacgctcgat atggcgcttt tccacttcgc
     7381 gcaaggtcag cagcggctcc aaggtttcat cggcaaacgc cttccgatcc tccacgtcca
     7441 tcccatcatc ggggtcgtcg tctggaacaa tgggaagaac atcagccgtc aaaacgtccc
     7501 ctttgcagag aaccaccccc cgcgtcagcg cattctccag ctcgcgaaca ttgcccggcc
     7561 aacgatattg cagcagcgca tccatcgctt cggaagaaac gtatcgaacc tgtttgtgag
     7621 tctcccgagc gatccttgcc agcaaataat cgaccaacat cggaatgtct tcccttcgat
     7681 cacgaagcga cggaagaagt atctcgaaga ctcgaagccg ataatacaaa tcctcccgaa
     7741 aactgcccgc tcccatcatc tcttcgagat tacgatgcgt cgcggccacg actcgcgctg
     7801 cgagcttgat gggacgagaa tcccccacgc gctcgaatac tcgctcctga aggacccgaa
     7861 gaagcttcgc ttgcaaatca ggccgcaact ccccaatctc atcgagaaat aaggttccct
     7921 gcccggccag ttgaaacctg cccgtcttat ccgccacggc acccgtgaag gcaccacgca
     7981 cgtgaccaaa cagctcgctt tccagcaaat caggaggaaa tgccgtgcaa ttgacagcaa
     8041 tgaatggtgc gttcgacgca cctgacgcgt gatggatcgc acgagctacc agttctttgc
     8101 ccgttccact ctcccctcga atcaataccg tcgcccgact cgtcgccacg gcaccaatac
     8161 gcttatatac ctcgcgaatg gcctcggatt tgcccacaat actgtctaca ccacgatcct
     8221 ctcgctcctc agctaccata tgcgccaagg tcaaggatgt tcttcgctgc tcgagcgccc
     8281 gttgcaccgt cagtttgatt cgattcgcat ccaacggctt gaccaagtag tcatacgctc
     8341 ccagcttgac cgcttgcacc gtcgtatgca tgtcatcacg cgcgctgatg atgatcactc
     8401 ccggcggggt cgtatccaaa ggccacgcct gatgcatccg gcgcaacact tccatgccgt
     8461 ccaccccagg cattcccaaa tcgagcaaac atacgtccgc cgtgccttca caaagccgct
     8521 cccacgcgct ttccccatca ctcgctgtca caacgacata acccgcgtcc ccaagatact
     8581 tctcgagcgt acgacgaatg gcacgatcat catccacgat gagaatcgtg ttcatgcctt
     8641 cttgaacttt gtcggtttcg gacatcgtgt gcatcaggag ggggacgata gcgccgcacc
     8701 atctgaaaag gaaacgcttg tgccgactac tcctcggagt tgtcgtcctt cggtttgtcg
     8761 tccttcggtt tgtcgtcctt cggtttgtcg tccttcggct tatcgtcctt cggcttatcg
     8821 tccttcgact tgtcgtcctt cggctcgtcg cctttcggct tgtcgtcctt cggctcgtcg
     8881 cctttcggct tatcgtcctt cggctcgccg cctttcggct cgtcgccttt cggcttatcg
     8941 tccttcggct cgtcgccctt cggcttatcg tccttcggct tatcgtcctt cggctcgtcg
     9001 cccttcggct tatcgtcctt cggcttatcg tctttcggct tatcgtcctt cggcttgtcg
     9061 cccttcggct tgtcgtcctt cggtttcgtg tctttggctt tcttttccgc gggcgacgtt
     9121 tcggcttttg ccggtggctc gggatcggct ggccgttgcg gcgctgggtc gatgattcgc
     9181 gaaggctcga gacggtcttg cccgcccggc atggatgtca cttcgatatc ttcgaacaca
     9241 cgggtttgac aggccaacct acgtccgtcc cccatgccgt gaaaccgttc ccggaaattg
     9301 ggtggagatg tagcgttacg gctgctttcg cgaacccata ctttgcaggt cccgcataga
     9361 ccgcgtcccc cgcaattgaa tccgctaaaa aactcacgat tgagatagat ccccgcttcg
     9421 agcgcgactt cgcgaatggt ccgcccactg gcgatatcga cttcacgatg ttccacgaga
     9481 aacttgattt tcggcatggg tggaccatcc gtaccgctgg ggtccggttg gcgtcaaggc
     9541 gaggcttcgt gcaagctcca aacatccttt cacgcgtccg acagtcccct tcgccgtcct
     9601 gccccttcca gacaagacga ggaagcggac tcgaaccgtg gcacggttag gagccacccg
     9661 tcgcaatagc gtgaaatgca tgagtgtact agggtaccca cccatgaaca ctgcgaaaca
     9721 aatcacgtgg catgagccgc ccttggcgga gacggatccg gaaatctttg ctctcatcga
     9781 gcgcgaagag gcgtatcaac gcgatacggt tcgcttgatc ccaagcgaga actatgcgtc
     9841 gcggggcgtg ctcgaggccg cggggtcggt gttgagtaac aagtactcgg aaggctaccc
     9901 gttcaaacga tattatgagg gacaagcgca gatcgacgcc atcgaggaac ttgcctgcaa
     9961 acgcgttgcg gaacttttcg gcgccgagca cgtgaacgtg caaccgtact ccggcagccc
    10021 tgccaatttc gccgtatacg ctgcattcgc ctcaccgggc gatacggtaa tgggcatgat
    10081 gctgccggcg ggtggccatc tcacgcatgg gtggggcgta tccgtgaccg gctccttttt
    10141 caaagccgtg caatacggag tgcgagaaaa cgatcatctc atcgattacg atcaagctcg
    10201 aaatctagcc aaagaacacc gtccgaaaat cctgttctgc ggagccaccg cctacccacg
    10261 tatcattgat ttccaggcct ttcgttcgat cgctgatgaa gtcggcgcca ttctcgtcgc
    10321 ggacatagcc cacatcagtg ggctcgtggc ggcgggcgtc catcctagcc ccgtcggtct
    10381 tgccgatgtc gtcacgtcca cgacccataa gacatttcgt ggtccccgag gcggcatgat
    10441 catgtgcaag aaagcgcatc gcaaagccat caacaaggca gttttccccg gattgcaagg
    10501 gggaccccac aaccaaacta ccgcggccat cgctgtcgcg gcacgagaag ccgcaacacc
    10561 ggaattcaaa gcctatgccc aaaagatcgt cgaaaatgca aaagctctcg cagaggaatt
    10621 gaatgcaaga ggcttccgac tcgtgaccgg aggtaccgac aaccatttga tggtcgtcga
    10681 tctaaccaac aaaggggttc ccggcaaaac cgcggcgaaa gccttggatc gagcggggat
    10741 cgtcgtcaat tccaacacgg ttcccttcga ccctcgcact cctttcgatc cctcgggtat
    10801 ccgtatcggt actcctgcca ttacgtcgcg cggcatgggt atgggagaag ccaaacgcat
    10861 tggcgcttgg atcgatgacg tcgtgacaca ccatgaggat gaggccgttt tgacgcgcat
    10921 cgctgctgaa gtaaaggaga tgtgctcgca gttcccggct cccggtcttc ccatcggcta
    10981 gaccaatctc cgaaaaaccc accgtcgtca ggagctgcct gcccctcccg ctgactatac
    11041 gtatacgtat attacgtgat cgacttgcgt cgtcgtgcat cgaaagtgtc ttcaacgaat
    11101 ggggcgcaga attcgaatga tactcacccc gttccacacg gcgaagaaag caaagagaac
    11161 caccagcgca atcgcccatc gggaggcccg aggcgatgcc ccatcgacga gaacccaagc
    11221 atccggtgca atggtttgtc ccgtttgctg aacgacagag cgagtcacgc cagaaaggcg
    11281 caatccagcc ttcgacaagg gaaccaaccg tccggtaaac tcgctcgggg gcacgaaacg
    11341 aggtccttcc atcccttccg gtacgcgaat ttccacccaa acccgcgccg ttcccgcaac
    11401 gggttgaaga cgaaatgaat cgccttcgaa cggccgtgca taacgaacgg ctcccgccgt
    11461 accgagcagc ccccgggcca cgacatattg attgggggtc agcgaggatg gttcgagcga
    11521 agcaagttcg ccaatggcaa tgggaaacga cgaagatacc gcgtattgaa cctcgctgcg
    11581 caacgaccag cccatggcgc aggaagccac catcgtaagc agcatcagca cggccgcgag
    11641 cgtccgctgt cgcttgggcg gcccgggtaa ggcttgcaat tcgggatctt cgacctcttt
    11701 gcgtgccaag gcgcgtgcat cggacaacga aggaagatcg acaggttgat ttagcggcat
    11761 aacgtaggac cggatgtcag gttggcgcgt tcctcggcct cagacaacga taaaacgccc
    11821 cggttcccat ccttcatgac acccctggtg cagaccccac ttcttgcggt aggctgagag
    11881 gctcggaacg agggaacccg atggcgaaaa gcaagaccgt cctctgcccg tcctgtggat
    11941 ttaaaaattc gtctacccaa aaacgctgtg tgtcgtgtgg agctaagatc gaacaactcg
    12001 gtcccaccgc acggagccgg gaagaagaac tcgagcgacg ctaccaacaa gaaggtttca
    12061 gcctcatctg gatgctggtc gctcttgtcg tgcaaggcgt actcaccgcc gcaatcgtga
    12121 ttggcttgcc catggtcgtg caagccttcg acttcgaagg gatctacggc atgtacatgt
    12181 gtgtgccggt ttggttcctc gggggtcttt tggtgggtct catctcgccc ggaaaaacgt
    12241 tcattgaacc ggtggttgcc gcttttttca tcgcggtccc caccgtgttc taccttgtgc
    12301 aaagccaaac cgttcgcacg atgcccacgt tcatgtacgt tctcatcggt tgcgtgggcg
    12361 tgatgttcac gctcattggt tcgtacatcg gtgaacgcat ccaaatgggg ccacctccca
    12421 aattatcaga ctgatcggtt cgtttgacgt acgaatcatt tcgccggctt cccttcgtcc
    12481 gaaacgatgc ccggcatcga gggtccatct tttgccaaag gacgacaacg cactaccgat
    12541 cggccatccc tgcgcgccac gctcggcttc gtttcccctg ctcatggtgt cgaacgcagg
    12601 aatgtatccg gactgacggt ctcatgctac tgcatcgatc gtgcatcgac tcagtgcccc
    12661 gcctccgccg aaaatcacat cgctcagccc gcggcgcgat gcgtatatcg atctgcttga
    12721 agacagccgt ggtctccgac gagaacaaca acaagcccgt gcaaactggc tttcgacttt
    12781 gtccccagag cgccaggagt tggttttcga actcgaggta ttgctcaaag gagcggcgtg
    12841 tttctccaac ccacgtaatc atcctggcaa accgcgacgc gtaccgacgg tggcccaaga
    12901 ttttcgtgag cctctagcgt tgctccacaa aggattcaaa agaatcacac accttgctcg
    12961 gctatgcctt ggagaccgcg acaaagcatt cgtgtttcac cgctatctcg aaactctcct
    13021 tcctgaagac accgctcgaa cccgcctttt tcgcgaggac agctcgcaaa gcacaccgca
    13081 agggagcctg ttcgcattgc gacgcggatt ctccaacctc gtggaggttg gcgatgggtt
    13141 gttacgtctt ccgcgtgtgc cgtttcggtt gttctatgcc accgcgaatc tcgcgcagcg
    13201 ggaggtgtcg caaaacgcat ttttcaatcc cctttcggct ttggagtttc gaccggagtt
    13261 cgatcgaatc caatccaccg aagtcgtgga gatgatcggt cgcgttcctg gagaacatgc
    13321 ccaacggttg acgtcgttga cgttcctttc gctgtttcga atgcttcgtt acctgcgatt
    13381 gctcgaacac atcgtgccag acttatccga ccctctcggc acggcagccg gcagcctgta
    13441 tctggtactc agcgtgctgc gctccgacgc tcgcgccctt gcgacctatt tgcgtcgaaa
    13501 atccggcgaa caactcgcgt cctctttcga acgcgatctg ctgatgacgc cttcttcgca
    13561 gcttcgcgat cggtatgagg aactactcac cacgggacat cgattgatcg acgcacgagg
    13621 cgctttcgaa ggcgtgagcg ccaatcttcg actcgaactg caacgcgttt tcgaacatac
    13681 gattccttca cccgaagagg gcagtggttc gactcgactg cgcgacgacg tactcacggg
    13741 catcgccgaa ttgcgtccga cattgcagaa cgtgatcatg ctgctagcgc gaacgctcgg
    13801 cgccacgctc gaggaagacg tggtgttcga tgatcagttg gcgaaacgcc agatatcgga
    13861 acggctgcga cggcatatct ggatgtttgc ccaaatcatt cgagcgttca tggtcaaggc
    13921 acgacacgca caaggcgccg gagaagcgtg ggatacggtg tcgagttttc aattcgtgcg
    13981 tgagtttgtc gcgtactttc gcgccatggg atacccgctg ctgagggcca gcgactaccc
    14041 tcgattcgca tcgttcctgc atgcgatgaa cgcattacaa gatgccgacc tgcttgatcc
    14101 ggacaagctc gatcgcgcga taattgaatg cgaagcgttt catgctttct tgtccgaatt
    14161 gctcgaacgt gtggggcagc gggaagaatt gcaagggatc gcattcgaca aacgtgcggc
    14221 tgccatgacg ctgcgcttgt acctgaggga gtagcgatga cccgtctatc cgaatatgga
    14281 tagaccggga tgcgttggcc acgggcagtc tcgcgtccgc gacaccacca cgaaggggag
    14341 aagccgtgga agcggtgaca attatttcca attttaggaa ttaagggaca gaccaccaat
    14401 tgcggcgagg ttgaacaggt gaaaccgccc cggacaattg ggtgggcgaa tcgagtctta
    14461 agcaataccc acagcgcttg tgggtcctac agcggtctat tcttcaccgt cgttgtagga
    14521 cgctacgggc gctatcagct ctttggaaga ggcttttcga ccgcggatcg tttttttggg
    14581 ctcccgcttg accgatcgtg ggcttgcttt tttgaccgcc tttttgggcg cttttttagc
    14641 gacttttttg gcagcctttt tgccgacctt tttcgcgacc ttcttcggcg cctttttcgc
    14701 tgccttcttg acagtcttct tggcaacctt cttggcaact ttcttcggcg ccttcttcgc
    14761 taccttcttg gcggtctttt tgacaacctt tttggcaacc ttcttcgccg ccttcttcgc
    14821 cgccttcttg acagccttct tggctacttt ctttgcagcc ttcttcggcg ccttctttgc
    14881 ggctttttta gcgacctttt tcggagcctt tttcgcggtt ttggtcttcg ctttcgtcgt
    14941 tttcttcaca gcagccatgc tagaccctcc tgtatggtac ctcgctcgcg gggtaacgca
    15001 tattgcacga cttatatggc catgcattgt gcgtgtcaat gaaaaatgtt cacgggtcgt
    15061 gcaaagggac ggtcttttct gcttcgacac cacgcaacgc ctgcatacag ccaaattgat
    15121 ctaccatttc aacgagatcc ccgcgaccgg gacgtgcctt ccagacgttg acttgcagac
    15181 ccccaacgct gtacataacc acggtcggct ggcacgtgac agttccgatt tgcccgccgg
    15241 tttcgctcac gattgcatgg cacaacggaa acctcgcgtt tcgcagtccc gctggaaagg
    15301 ttcaacaatg gcctggtatc gctcggtcgg attgtctttt ctcacgacgc tcttgctagc
    15361 tttcgttctc ggaggctgcg gccattccga ggaagaatgg caagcgaaac tcaaggaagt
    15421 cgatggactc aaagcgcagt tacaagccga acggcaggcc catcaaaaaa cgcaagacga
    15481 ctataacgcg gcaaaagcgg agatcgatga actcaaagac cgtctgaagc aggctggggt
    15541 cgacgttcag caactcaacg cctctctcgc cgaacaacgc aaagccctcg aagagttcaa
    15601 acgcagggcc gaacagctcg aagccatccg gaaacgcttc gaattgcttc gaagcaagct
    15661 cgattccctc gtcaaactcg gactatccgt atccgttcgg aataaccgaa tggtcatttc
    15721 catgcccggc gacgtgcttt tcgactccgg aaaagtcgac ctgaaaaagg atggcgaagc
    15781 gattttacga caggtggccg aggttatccg cggcgatgcg caactcaacg caagaacctt
    15841 ccaagtagcc ggccatacgg ataatcggcc cctcggaggg ggtccctata aggataattg
    15901 gggcctttcg ctgatgcgcg cgcgccaggt cctcattttc ctcgtcgaac ccctcgataa
    15961 agaaggcggt ggcctcaatc cgctgcgctg gagcgccgcg ggctatggcg atacagaccc
    16021 catcgccccc aacacgacac cggatgacat gcggaaaaac cgtcgggtcg aactcatcgt
    16081 cttgccgaat gtcgaagaga tgctcgactt gcaaagcctg actcgctgac ttctgcgccg
    16141 ggctcacgat ttccatcacc cttccccacc atcgaggttt ccgcttgaaa gtcgctacca
    16201 tcgacatcgg caccaattct gtcctgctgc tcatcgcgga aattcacaat catcagctcg
    16261 tcgccctgca ggaagaatca accatcaccc gactcggaca agacgtcgac cacagccgac
    16321 gccttcatcc gcaagccatc gaacgcacta tttcttgcct acatcgctac gccgaagcca
    16381 tccgagggca cggcgtaagc caaatggacg ccgtcagcac cagcgccacg cgcgatgccc
    16441 aaggagtcga tgactttctt gatagtgccg agcgcgtgct gggtgtccgt ccccgcatca
    16501 tctccggaga taccgaagcg cgactgtcgt ttcaaggcgc attgagcggt ttagacgtgc
    16561 aaggacccgt gatggtgtac gatgtcggcg gcggcagcac ggaaatcgtc ctcggccatg
    16621 tcgaccaggg ccgtcgatgg cacattgagg cctcccatag cctcgacatc ggatgtgtgc
    16681 ggctttccga acgccatatc cgctccgacc ctcctgcgga agtcgaactc accaaccttc
    16741 gaaccgaagc agcgctgctt tttggcccaa tgagcttgga tgcccctgct cgaaccctca
    16801 tcggtatcgg gggaaccgtg accacactcg cggccgttca ccttggcctc gcttcgtacg
    16861 accccaccaa aatccacggc gctcttttga ccaccacgca aatctcccga ttggctagcc
    16921 gtctcgccca actgcccctc gcagaacgac aaattctgcc aggaatggaa cgaaaacgag
    16981 ccgacgtcat catcgccgga gccatcatcg tcgaacaaat cctgctatcg acaaaatcac
    17041 ccgagacacg ggtctcaaac cgcggcctgc gatggggtct cgcgctcgac ttgatagctc
    17101 cacacgtata aaatgcctca tcccatcatt tccgcttgac aaactcggca aagcgcgact
    17161 aacatctgtc gcttgccgca atttacccta atttctgacc attcactcca tgagagtaaa
    17221 ataccgggtc tttcatgcaa cgtaaatttt tgcgggaata gtccgttgtc ttgatggttc
    17281 ctgcctcaac gttgtgcgtg ttccgctacc tgcgaaacgc gggggtcttg cggccgtcaa
    17341 ccaaggagac gccaatgcag gctcgctcgg aagtcaaatt caaagttgga gataaagccg
    17401 tctaccccgc ccagggcgta gcagaagtca ttagcatcga agaaaaggat atcgcaggct
    17461 cccgtcaacg cttctacgtc ctgcgcatca tggacacaga ccgaaaaatt atggtccctg
    17521 tcaataacgc cagcgccgtc gggctaagac aagtcatctc agaacaagaa attcgagaaa
    17581 tttttgatat tctgcgggag cgaaccatcg ccttcgacaa ccaaacctgg aaccggcgat
    17641 accgcggctt catggacaaa atcaaaaccg gctccattta cgacgtcgcg gaagtcttgc
    17701 gggaccttta tcgcctgaaa accgataaac agctcagttt cggcgaaaga cgaatgctcg
    17761 atacggcccg aaccctcatc gtcaaagaaa tcgccatctc gagaggacaa accgaagaac
    17821 aagtccgcac cgaaatcgaa gccatcttcc tcgccaacta acctgaaatc cgcctgcccc
    17881 cggccctaac ccctcaagct tacccctcgc gcctatgcat agcctttcac cctcccgcgt
    17941 ccggacgctt ttccaccgcg ggcaaaggcg gtagcaccaa atccacgcgc cacttcccct
    18001 gctccagcac acacggcacc tccgcacgct ccagctccgg gtccgaaccc accacttcca
    18061 ccaacgcccg atcccctgcc acccgcgatc gcattcgcct cggtgtaaaa cgcaacgaaa
    18121 accgcgatgg cgcaagcata ctctccggct ccatcttgcg accggtcgcc gccgacgccc
    18181 ggcgcgcccg ctcctgtaag ttgctcttcg tctccgacga cagcaattca tacgcctgct
    18241 gcgcatcacg cggatcccca tgcacccgcg ccatccgctc caaccactcc tgtacgaccc
    18301 cctccggcgt ctcgtccaca tcgcgccgtc gacaacccac ggccatgccc gcaaaccatg
    18361 ccaatgcctg ccgacgtgac agccgaacct cctttcggat ccgaatcccc agcttctgcc
    18421 cagcttcccc cttcatcttg cgttccttcg atccatcgat cgatgccata gaccagttat
    18481 ccccctggtg tcatccccgt cctcgaccac gcctattcga cagccaagaa ttaggacttg
    18541 tgtaggttcg tagaatacaa tgtaggcatg aagccgcgcg ggcctcgcca agcaccccaa
    18601 aacacaaacc ctctacccgt ccctcacgcg tcggacctca ggctctttca cggtgataat
    18661 ctcgacgtcc ttgcggctct ctccccgcaa tttcaaaaaa aattcacctt ggtctatctc
    18721 gacccaccgt tttttaccat gcgccagcat gaacgggtca tccgaaaaac aaatccccat
    18781 ggccctccaa tccgtctttt taccccggcc ttcgacgatc gatggcagga ttttccggat
    18841 tatctgggct ccctacgcgc tcgtctcgaa gcccttgctc ctttgcttgc accccatggc
    18901 agtctggtgc tgcatgtcga ccctagaacc agtcactacc taaaaattat cggcgacgaa
    18961 atttttggtc ccgaatgctt cgccagcgaa atcatttggc gctatcgccg ctggccgacg
    19021 cggaccgcga atttccagcg aatgcatgac gtacttctgc ggtgggtggg ggacccgcgg
    19081 caacctccga gattcgttca gcaatacgag ccgttggcgg cgtcgacctt gaaaacttgg
    19141 ggaaccggca aacagaaagc cgtattcgat gatggaggtc gtcggcgacg ctcttccacc
    19201 gaatcgcaac agtctcctgg tgtccctctg ggggacgttt gggatattcc cattcttgcc
    19261 ccggtcgcca aacaacgaac cggttatccc acgcaaaagc ctgaggcttt gctcgaacgg
    19321 ctcatccgat cctgctcgtt tcccggggat tggattctcg atccgtatgt gggaagcgga
    19381 actaccctcg cggtggcagc ggcgttgggc cgtcatgccg tgggaatcga cgccagtgcg
    19441 gaagcttatc aggtgtctca acagcggctc cggcaagcag ggcatccgat ttcaacgttt
    19501 tcaacgacat caaattccaa tcacacgcca ataggctcga gatcttctgc tcaggtgtct
    19561 tgacccccgc gccgttcttc gccacacctt cacaccatga aacgcgcagg catcctcgtc
    19621 ccatgcatag ggcttggtct ttccctcatc gctttgatgc tgctggcgag ccccaaccat
    19681 agccgggcga gcaaaagccc ggatggctcg acccgtgtcg ttgcgaccgt agggcaggca
    19741 accatcaccg ccgcggagtt ggagcgtcgt ctcagcgcgg ttccggcgtt ccaacttcgg
    19801 gtatacggct cgacgccgga ggaggtgaag cgaaactact tgtctcgggt gctcgttccg
    19861 gagcttcttt ttgcgcaggg ggcgatggat cggggaaagc acgaagctgc cgaaattcgc
    19921 tcccggcagc gtgacatttt gaaaaccgcg ctgctcgacc aattgcggca ggagtccgtg
    19981 caacaggaag cggtaacccc agagcaaatc gcggcgtatt atcaggccaa tcgcgagcgt
    20041 tatcagacgc ccgcgcgggt cagtgtgtgg cgaatcctcg tggcggacca cgaactggcg
    20101 aaaaacctga ttgagcaggc gcaaaagaac ccaaccccca aagcgtggac ggagctttcg
    20161 cagaagtatt ccctcgacaa atcaacgaat ttgcgcgggg gcaaccttgg ttttttggcg
    20221 gccgacggta cgagcgcgga cggcaaaacc cgggtttccg agcccattgc caaggctgct
    20281 ttcgcggtgc gagatggcga gattgtcaag gagccggtcg ccgaaggtgc gggatttgct
    20341 gtgatttggc gcagaggaag catgcccgcg gttcatcgaa ccgtggcgga cgaagcgaaa
    20401 gccatcgaaa aaatcttgct tcgcgaccgc ttccgcgcca agcaagacgc gctgatcgaa
    20461 cgtttgcggc gagaccactt gcgattgctg atgccggatg gaccggaatt gatcgcggta
    20521 tcaggaggcg ggcagattga gcaggagaga aaacccggtc gtatcgttcg ccgtccgggg
    20581 cgcacggagc ctttcgccac gccgcgcggt cttcggtgaa cggaaacgtc ggggagcttg
    20641 cagcgccgca ggggggttca gcttttcggt ggcttcggca tgccgtggtt gggcagcgct
    20701 cggggccgcc gctcgatgat agttcggcag ggtaaacccc gccgcttgta gcaccgcttg
    20761 aaacgttttt gcctcccccg aggatgtctg aatctgccgg atgaaatcag atatgcgccc
    20821 atcgatcgcg gcctgcatgg ctcgctccat cgcggccaga gccagcaaca gatggggatg
    20881 agcttctcgc acttccgggg gaacgaccat ggtggatgcc acgtccagac gggcctgcgc
    20941 cattttttgt gcgtttttgg caagttcctt gtctccaagg ttggtgcgaa cccatcgtcc
    21001 cgcatggtcg ctttccacca gcagcatcgt cgaggtattc aaataggggc ccgcccagca
    21061 gggaaaagaa aaaagcagca tcagcgcaac ggtgctgagc gcgagcggca ataaaaccgg
    21121 cagtctacga ttcatgatgt tttctcattc gatcggtggt tgatcagcgc catccgtgcc
    21181 gcggcctgct ccgcgctttt tttggaacgt ccttgcccac ggcctagcac gatatcctcg
    21241 acacgcactt cgacatcgaa gcgcaggtca tgatcggagc cagaaacgga aaggacgtga
    21301 tagacggggg tggcaccgcc gcctcgttgc acccgttctt gcagttcgct tttgggatcc
    21361 cgttgtgcga ttttaccgcg tcgcgtcatc ggctcatcca cgattcgacg ggtaagtttt
    21421 cttgccccat cgagtccgtg ctcgaggtaa acggcgccga cgagagcttc gacgacatcg
    21481 gcaaggactt tcggctggag tgcatcgccg gacaaggagg ctcctttgcc gagattgacc
    21541 cactgggcga ggtgaatgga atgggcgaag tcggcgaggg cctgggtatt gacgagcgaa
    21601 gcgcgcatgc gcgtaagctg tccttccttg gctgtcggag cgtgagatag gagcaattcc
    21661 gtaacacaca aaccgagaac ggcatctcca agaaattcca ggcgttgata gtctgattgg
    21721 ccgggatgtt cattgacgaa gcttgggtgt gtcatggctt cgtcgaggcg cctcacgggg
    21781 ccttcgatat ccagcaattg cagaagtgca gttcgatcca tcatggtggc ctatatggta
    21841 attagcatac gttatagcgg gagtccgtcg acgcggatac aataaccaag catggcgaaa
    21901 atcggctcat cggggcgcac gcatttcttg catacccatg caatcgttgc agaaacgcga
    21961 cgatagcagg gggaggatcc tcggggattg gtggcgtggg tcacctcccc tacgcggcat
    22021 gatgcttgct ttgcaccagc atggatccat gcgaatgcaa aagcccccca tcgtagctcg
    22081 aacgaacgag catttccgct tatcgcgcgg ttttacgttg ttggagttga tgacggtggt
    22141 gattttggtc ggaattatcg cggccattgc gataccgacc gcgagtcggc tgatgcgtga
    22201 aaaccggtca tcgacggcag cgcagcggat agcgttgatg taccaggtag ccagggcgag
    22261 ggcgatggga c
//
LOCUS       DAOSOR010000023        21757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_2393_length_21757_cov_4.287439, whole genome shotgun sequence.
ACCESSION   DAOSOR010000023 DAOSOR010000000
VERSION     DAOSOR010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 21757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21757
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2393_length_21757_cov_4.287439"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /locus_tag="PK710_02335"
     CDS             complement(<1..1459)
                     /locus_tag="PK710_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOT08574.1"
                     /translation="MWSPESRKQNAEELRRTADKLLRYRQLADRFHGYFKDDSDNARL
                     LVNLRAKLEDLRGRALDRQKRLAKGVVKIGVVGLEKQGKSAFLSAWLDSEKLLPSEAE
                     RCTWSTTVVEPGAQGEYRAVVEFYQDDEFKKRIESYFDALEPGSAERWAGLSNTEIMR
                     LKTAFRDREGFDIDDPDRAGKREQLALAELMEITNDLKEIKAKLNQPPIKIEASSMDE
                     LADRIRPFIALKDTMHGNRPYPGVRAVKVVTVTIPVRGAMPGVVLMDLPGIDAPSDKA
                     RRDTEEALAEEVDVTIFVKDITRPSLVRHELDLLRTAQTADRSISLKDRMFVVLTKTD
                     LFDQPDENGNWHWALAVRNFKEQGIDRVYPYSKIWVHKGPDTQHPVARHLMDFYGTTQ
                     PVHGLDRLQNAISRYLSTDVEAIDRKVTDSIYTEFKELENQLRGALVSVKDALSDREF
                     ERRAEQVFDEHFEHIRAGEDPLGLLPEIRKRLSRFM"
     gene            complement(1632..4418)
                     /locus_tag="PK710_02340"
     CDS             complement(1632..4418)
                     /locus_tag="PK710_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08575.1"
                     /translation="MASSSTDPTAHVQRSSTGQYSPRVRIDDLAILIRRISSDGIRYE
                     LKPSRHMTVAENVVLPIFDGWMQGGELVVRASGLCDGPFEVSLDVDGNRQTSQVAAHV
                     GQSSSAYLHFSFEVVVPLRALAPFIGASIRLGVAISPGERVLRLLRTTLFPMRAAGPH
                     ISNLDLAEATEALLFDAPERHLFDLQNPEEGLWVGTGAWRLRLMAEWNRDDADAYQLE
                     TRPSRLLHRWQRTNDISDVLWEDTTRRAGGRHTSTEFIFDSSHDDIGLIPPEGRDVPL
                     DVIVEHELSFADHRCVMTWHAPVPLRLRDPVPHLKRFKRLSAVGIDFGTTSTVAAFHH
                     KGFRSLLRLGGKDNDESWENPTYLLVEDHQRLWDEMTRASGGKRFPNLMRVVLASHAA
                     YEKMAESPNAVVGELKSLPERVVILDQSPQLRDRQQQADFLLDEPRVRTLIRTYAYLL
                     GRAINRPGQDVFLHYWLTHPAKFDKRTRALLEEEIRAGILLSIPEGISADEVTVSMRA
                     SEPEAFAAEVCPELAADPALEELISKYGELRFAVFDFGGGTLDIACGRFRPASDTETA
                     ESGASTVIETLQVGGDDHLGGDFLTHEMVWLTHQFDKHLPEMESKEVPMMRPQTVPPN
                     TLSSKPHLYKRSLAGRQNMVRFQREMELEAVKFKRSHAPKRVEELSAARLDGSEVSLT
                     SLKTDIAGLHAGLTGHLKDRIRDGARLLSSMLRNTSWGTGEDWRDQGVVLLLAGNSSR
                     SEYVEAALAEELGMPGLKVWRPGGNTPFQQVVLYETPTRIERGVRTVGVTPKTAVALG
                     ALRIANREVHLVRRAQGFSYFLGDLRGFPPKFVALVPMGSLPADPSNFGPHFVDFGTW
                     DGQKPFRVCKDYDPGTMTSKDPRLSIVPTNLPIEASGRLYVCVVAPDEVLLHLDRREE
                     EPLRTTLNLAKYMD"
     gene            4602..5411
                     /locus_tag="PK710_02345"
     CDS             4602..5411
                     /locus_tag="PK710_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08576.1"
                     /translation="MAELVAVIVSFPAVVPTVLLGVVLLYWVMVVVGVLDVDFMSGEG
                     AADGVLEGVQGVDALVQGAPDAAVEGIVDGAAHGITEGVADGVVEGSVDGVKGLVEIT
                     HASATPMVTQGVETVNVLSFAHFRSVPVTVSASVVTVFSWLFCVLSVRILSPFLGQPS
                     WVPGLGMLLASVVLALPPSALLLRPLANVFRVQHAKSFQDLVGHVCVVTTGTVNDKFG
                     QARLEVTDLDLLVQVRTEQVRLQRGDRGLIVAWDARREVFIIEPYDKLLEG"
     gene            5507..7711
                     /locus_tag="PK710_02350"
     CDS             5507..7711
                     /locus_tag="PK710_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08577.1"
                     /translation="MSDLIPIIIAIVALLLLALGAAAIIARFYRKVLQGQALIVNTMK
                     SEPLVTFTGAVVFPIIHRAEVMDISVKTIEIDRRGKEGLICQDNIRADINVTFFVRVN
                     KTRDDVLKVAQSIGCVRASDHETLRELFSAKFSEALKTVGKHFEFVDLYTKREEFKDQ
                     IIRVIGTDLNGYVLDDCAIDYLEQTPVEALDKDNIMDAEGIRKITAQTTEKNIQTNEL
                     RQKERMEMGSQNLTADEAIFRFEQRRAEAEAKKAKEIAIAEAREQNEAARVASDEGKK
                     TAVVRYKNEEEALVAAEAKARAVAVAEKNREREVAIETERVEKARQLEIISREREVEL
                     TRIAKDKDVEVQKKEIADVVRSRVAVDKTVAQEEEYIKDLRVVAQAKRDKEAITIAAE
                     AEAQEALVKQVKAAEAAEQCARFKAKEKLAIADADLEASDKQARAKIRLAEGVTAERS
                     AEGLASVRVKEADAVATEKLGLVDAKITLEKMQATAVGEEKQGLARVRIQEAEAEAIQ
                     KQGLAKANVVKETKLAEAVGEEQKGLAEARVREAEAVAIAKRGEAEATAVRERLAAEA
                     SGLSEKANAMKALDEAGRGHEEFRLRLEKEKIVEMQSIEMRRQIAEAQASVLAEAFKQ
                     AKINIVGGDGAFFDRFVKAATLSHTLDGAVDGSDTIKTVFREYLSGEKSLPEDIRQIL
                     TRPALSSGDVQRLSLSGLLMRLASGADDKARANIHKLLDEAKKLGIDDELIR"
     gene            7728..13319
                     /locus_tag="PK710_02355"
     CDS             7728..13319
                     /locus_tag="PK710_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair ATPase"
                     /protein_id="HOT08578.1"
                     /translation="MDESGAKNTDGAPTLEGSSYDVIRRRLLEQASELRSRTDSLNDK
                     RMRVFGSSELKLLSNERVRTENNSTPRDMVSISGHLLFGFQVFLGLKSETNVADILTL
                     YKVSPTSEGFDLTPTGLDAVGGFLASEDFQKEFRDAFRYSKEAELLQIRRTDQRLLLI
                     VQVGSTVSDVKVFRFSIDAAGRVRYLDARGEEDAVLPRPHDFAWKVTTREDQVPGAHP
                     HVSIEDAVFVETVGGDLTVKIEDNTRDGWGIYREPVDDPNQSLDDASIAYARLGALIV
                     LRVKPFREEAYRYLVFNTRTKGVARIDAIEQACLQLPEEHGIIFPGGYCLRTGETKRF
                     IEDPRGLQYLSSIRSPNGEDVLYVFQRPTDGFYHLMPYNLVRKEVANPIACNGFSLFD
                     DGKMIVFRAAAEPSRVHVVQVWQTPFTSAEFAATAPTDGSFLGKVGNADLVRGISDAY
                     TLVTLARYDTPSRASYEELIAACARFEDAYFWGNHAEAGNLKEAVSAMRGTTELIIDE
                     FEKVAAIRRRASEALGRAADEQRDLVNEIMASDLSSLDAYMGALTNLRKQRGKLISLR
                     EMREMDLVTVEALENEVVEQFDRLSQRCAEFLLGGEAFAPLTTRIEELQQQIEAVSKA
                     VELNALGADLLGVQEGLTLLSEIAGGLVVEDATARTRILEAISEVFGQVNRVRATFQA
                     RQRDLSGAEARTEFGAQFALFSQSVAGALAVCDSPERCDEQLSRMLVFLEDLEGRFGE
                     FDEFLSDLTMKREEVTDAFGARRQTLVDERQRKAQSLFSAAERILTGVIRRTGKMDSA
                     DELNAYFASDPMVHKLGDLAAQLDALGDTVKAEELRGRLMAARQDAVRAQRDRSDLFE
                     AGTEIIRLGNHRFSVNTQSLEATLLPRDGTMTLHLTGTGFYDPVRDDALLEAKDLWEQ
                     SLISESPEVYRGEYLAGSLLLAAEEGRDGLSMRILQDASLQEGGLSKWVRELAAKRYD
                     EGYERGIHDEDAARILEKLLAMRATAGLLRFSPKPRALACLVWAYLPRDDRTLLHRRA
                     RSYGILRDKLDDEGAQSSLGEQLEATVRGTAESFRIECSVSDIRAASRYLVLELGQDH
                     VRFSTSSEAIAVKDALLSHLDDLGARRAFEEDLRALDAFAAQRLELAQNYLKAFAKRQ
                     SERTIDDGLALEAAVLLISERLLERQSVAAVTSVVVTELLGQHPRIRERSMSLRLDEF
                     LDRLGVFVYERVPRFRKLREVRSAVVAREQQRLRLDEYLPRVLSSFVRNRLVDEVYLP
                     LIGTNLAKQLGAVGAAKRTDSMGLLLLISPPGYGKTTLMEYVASKLGLVFVKVNGPSI
                     GHAVTSLDPAEAPNLTAKQEVEKISFALEMGNNVMLYLDDIQHTNPELLQKFISLCDA
                     QRRMEGVWRGRTCTYDLRGKRFCVVMAGNPYTEAGTKFQIPDMLANRADTYNLGEVLE
                     GKADLFASSYLENALTSNPVLAPLSGRAPADVHLLVRLARGEEVQTTHLSYGYSEAEI
                     QEMVSVFRLLIRVQDVLLKVNQQYIVSAAQEDAFRTEPRFQLQGSYRNMAKIAEKIVP
                     AMNLQELDRLISDHYMAEAQTLTAGAEANLLKLGELRGTLSDTERERWNAIKLEYQRV
                     KRMGGREDDPVSRVTGTISGLDERLASIRETILEAAREAKKASKSNAFDASGGAILPL
                     LSSLEKGFRELSQPKVSLQVQQQPPPGFGDLLAQQISLIQNTLVPLVQTAMNRGGRSE
                     LLEARVAELTAVVRELASQRAPDLSPPPRFEVEVTLDGPSNFYVDNPQAGVLKQGGLF
                     VATVVKPPAVGQRVVVNVVFPWKQEHDIAGRVDWIQSQTPAADDALRQGFGVKLENID
                     ANTRVSILSFVKQRPPWVLTR"
     gene            complement(13494..14996)
                     /locus_tag="PK710_02360"
     CDS             complement(13494..14996)
                     /locus_tag="PK710_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOT08579.1"
                     /translation="MRKSTLLVSLPTIALSLYSFTARAETNACRVVQGTSSSFLWCEN
                     ELTQMSSAQRAARLMPAPSFPTSSTPPPQLRDLPNAVPTDPKFNWHNVGGDDWMSPVR
                     DQKSCGSCFVFGTLGALESQLKVIAANPWLDVDLSEQSVISCITLGSCANGGTAEEVG
                     NRLKTHGVPDEACYPYLAEEGQCSNVCSDWKERRVRIDDWHMSIIPWSVPEIKKQLVK
                     TPVISQMQIYSDFYGYKSGVYSRSSSAKPDGWHVVTIVGWDDSDNSWIVRNSWGSKWG
                     DGGYFKITRDTDCSLTLQGICFALHVNYLDVDPAETPGMPCLKDNSLHLQAKVGEKAS
                     GSVQITNCGKSWTVDVGIDSVPSWMTATLKNTNLGVGQTSPLTIEANAESLNVGKHEA
                     KIRVYGGPASSTLHVTFDVQPQPKPDPEPEPSEEPGPEPTPEAGPEPSLEAGSVPDTN
                     QPETAPPTGAAFDSDGGDDGCGCRTVPSQHRTMAWWALLITGLWFVQRRK"
     gene            complement(15116..17158)
                     /locus_tag="PK710_02365"
     CDS             complement(15116..17158)
                     /locus_tag="PK710_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04551 family protein"
                     /protein_id="HOT08580.1"
                     /translation="MPHIRRAAVFVVPMLLLSFPLSAQPKPEGELETSDDATSASQDE
                     SSETPEPASTETRRSESPPNAHPSLTPAPISQANAASSSSLISQHDAPAPVVENHEQV
                     FAEDWWHIARPILELHGYFRMRAELFQNFSLGRSDPYDKALWPRPPDHSYTSWFDGQI
                     QQHAAHACGEDAANPQYCTDKTQAGANIRFRLNPELHVSDNLRIMSQIDLLDNLVLGS
                     TPTGYANEPSSSGGFARVAPSGYTPLGVYNETMMPPVAGYNAYGNSITVKRVWGEYLT
                     PIGQLRFGRMPSHWGLGMVQNAGDGYDSDTQSTVDRLMFTTGIKALDLYVSAAWDFPG
                     EGAISQSAQQQGQAYDLAQLDDVNQYVLTLMRRKQPALQRLTLSRGGLVLNGGVQFTY
                     RHQFLANDAFNTTNAANLGASADTIQDGYVRRNAKIIIPDLWLQILYKKLRFEAEAVM
                     VRGSLDNTEVAGNNYENVREDENGHKIRQYGVATQTEFRTVEDRLSLQFGFGWASGDP
                     DVTGPQVGGLTPGSGGLQPQRTDDRTDSTFRFHPDYRVDLILHRHILNRVQGTYYFRP
                     SVDCDFSRKPNGQRFGGGGAVIWSRASEFVQAPGHRRDLGIEIDLQLYFQSKDGSLND
                     DPEKMGGFFTMLQYGVLFPLGGLGYLPGEEEQAANQNIRLETSAAHTLRWFGGIFF"
     gene            complement(17307..18458)
                     /locus_tag="PK710_02370"
     CDS             complement(17307..18458)
                     /locus_tag="PK710_02370"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT08581.1"
                     /translation="MTVCAGCKTVFSNDGRFCPNCGAPAPARIQAPTTGPGARLDLGW
                     SQVIVGNLIGDGGMGVVYQGWMYYNPHGPQANTPSHPVAIKILHPLMTTRDFVRKLFV
                     GEATALQRLNHPNIVHFFSLVEAPGQLSLVIEFVDGEALDNVIARHAPTARAYRQPCL
                     PFQRAWHYFEQLLGALAATHELGIVHRDVKPANLLIRRDGLAKLTDYGIARLPADEAR
                     RSGGVQPGTGAYMSPEQVLGRELDGRSDLYSAAIVLFEMLTGKTPFDEGDKSELMVRA
                     SQVEDTPPPITQLLPQAPPVMDLLFARALAKNPDHRYPSAIELGNAFCQALGIPETPG
                     WRAQREFAKRATGMVPAAMVPPRKKGGTQPIAQVDANAMRNHLANVYAK"
     gene            18639..20555
                     /locus_tag="PK710_02375"
     CDS             18639..20555
                     /locus_tag="PK710_02375"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT08582.1"
                     /translation="MRLRPLMMSCVLGTGMMLAAGVASAQQEGEFSVQRFEPAPGPNN
                     FVTVEGARVTGDMAWSVGLFGNYAHRPFVVRSCISETNCDEANKLLNDDIAVVRDLIT
                     ADVLASLAPISRLQIGLRIPLSYVNGDGVDTVRGQPATGGLSGFGLGDPTLEGKYRFF
                     GEPDTPLVLGAGAFVSGPVGHAMAEGKYIGYSSPVVGLRGIADFQAGALSLGGNLAAL
                     YRSSATLGSTELGPEFRYGGAVGYNVSPVFKLVGEGYGGTKFSTKNGTNSLEALGAVQ
                     IMPLRSGFSFTLGGGAGVIQGVGVPMFRGFAGILYSHTATDTDGDGIPDDVDQCPTEP
                     EDLDGFEDEDGCPDPDNDGDTIPDDRDKCPNQPETFNDYQDDDGCPDEIPDRDKDGIA
                     DSEDKCPDAGGEVIRVKGPHYGCPDRDKDGIPDHLDKCPDEPEDTDGYMDEDGCPDPD
                     NDGDGIPDVEDQCVDVPGPKDNHGCPYEDKDKDGIPDHLDKCPDEPENYNGYQDEDGC
                     PDNRPTLVTQTADAIEIQGSVEFATNSSKIVGAKSFQILDGVASLMVHNLRIQQVEVQ
                     GHTDNRGGEAANKKLSQDRAEAVVKYLTEKGVQASRLTAVGYGQEKPIADNNTNAGRQ
                     KNRRVEFKITRQAK"
     gene            20681..21727
                     /locus_tag="PK710_02380"
     CDS             20681..21727
                     /locus_tag="PK710_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08583.1"
                     /translation="MMGLVACCLALTSESFAQQTDYQGPSRAPHVRLLLDAHTWHGPW
                     TITFVNEEDVPVRIAADARQLSLLVRLPNDKNYTKCSLPSSMRGTGRDRQLVLDPAQS
                     YVETIDPRLYCWGENQTKLAPGASVTAFVGWAPDVRLEKRSKPQVAPFVVEPVLAPPA
                     FPFTKQMASLTHWLAASDDSLIAQPEQPPARYVGTPDLRLSTPKWEDADLRSARISAT
                     LTNVGDRAALIHIRPDDLELRVQRPDGSMAACGPGSSYRAAVRDFFLTIQPGKSVSVS
                     SLLSERCPSGTFERPGLYQLHTTLRARDNGSAFHLQAITGDFAAPTPTLLRLRTARLP
                     FCRRPRSLPRDSST"
BASE COUNT         4628 a         5733 c         6649 g         4747 t
ORIGIN      
        1 ccatgaatcg actcaacctt tttcggatct ccggcaacaa tccaagggga tcttcccccg
       61 ctcggatgtg ttcgaagtgc tcgtcgaata cctgctcggc acgacgttcg aactctcgat
      121 ccgacagcgc atctttgacg gatacgaggg cgccacgaag ctgattttct agctctttga
      181 attccgtgta gatgctatcc gtcactttgc gatcgatggc ttcgacatcg gtcgatagat
      241 accggctgat ggcgttttgc agacgatcga gcccgtgaac gggctgggtc gtgccataga
      301 agtccatcag atggcgggcc acgggatgct gggtgtcagg ccccttatga acccatatct
      361 tggaataggg atagacccgg tcgatgcctt gctctttgaa gtttcgaacg gcaagagccc
      421 aatgccagtt gccgttctca tcgggctgat cgaatagatc ggttttggtt agcaccacga
      481 acatccggtc tttcaaagaa atgcttcggt ctgctgtttg cgccgtgcgc agcaagtcga
      541 gttcatggcg aacgagcgaa ggcctggtga tgtctttgac gaaaatggtg acatcgactt
      601 cttccgccag cgcttcttcc gtgtcgcgcc gcgccttatc cgaaggcgca tcgataccgg
      661 gcaagtccat gagcacgacg cctggcatgg cgccgcgcac gggtatcgtc acggtcacga
      721 ctttgaccgc gcgaacgccc ggatagggac gattgccgtg catggtgtct ttcaaagcga
      781 tgaaaggccg gatccgatca gcaagttcgt ccatgctact cgcttcgatc ttgatgggag
      841 gttggttgag cttggctttg atttctttca ggtcattggt gatttccatc aactccgcca
      901 aggcgagttg ctcacgcttt cccgcacggt cagggtcgtc gatatcgaac ccttcacggt
      961 cgcggaaagc cgttttcaat cgcatgatct ccgtattgct caatccggcc catcgttcgg
     1021 cgctgccagg ctcgagcgcg tcgaaatagc tttcgatacg ttttttgaat tcatcgtcct
     1081 gatagaattc caccacagcg cggtattccc cttgcgcgcc cggctctacg accgtggtgc
     1141 tccatgtgca tcgttcagcc tcgctaggca gcaatttttc gctgtcgagc caggcggaaa
     1201 ggaacgcgct ttttccctgt ttttctaacc ccacaacgcc aattttgacg acgcctttgg
     1261 ccaggcgttt ttgacgatcg agcgcgcgac ctcgcaggtc ctcgagcttg gcgcgtagat
     1321 tcacgagcaa ccgagcgttg tcactgtcgt ctttgaagta gccatggaag cgatcggcta
     1381 actgtcggta acgcaatagc ttgtcggcgg tccggcgtag ctcctccgcg ttttgtttcc
     1441 ggctctcagg actccacata cgacatgctc cttccgcggt gttcaagggc gaattccgtc
     1501 atcccgcggc cactttctcg ttgaacaaca agcgaattgc acgcgaatca aacgaacaaa
     1561 catgcgctcg taaggcatga tcccggcgtc gaaagccgcg tgcgaagttc ggttccaaag
     1621 ctgcatcgcc atcagtccat gtacttcgcc aggttgagcg tggtgcgaag cggttcttct
     1681 tctcgacgat ctagatgcag caacacctcg tcgggagcga ccacacaaac atacaaccgt
     1741 cccgacgctt cgatgggcag attggtgggg acgatggata gacgagggtc tttgctggtc
     1801 atggttcccg ggtcatagtc cttgcagacg cggaacggtt tttggccgtc ccaggttcca
     1861 aaatccacga agtgcggacc gaagttcgag ggatcggcgg gaagggaacc catcgggacg
     1921 agcgcgacga attttggagg gaagccccgc agatctccga gaaaatagct gaatccctgc
     1981 gctcttcgaa ccagatgcac ttctcgattc gcgatgcgaa gagcgccaag cgccaccgcc
     2041 gtttttggcg tcacgcccac cgttctgacc ccacgctcga ttcgtgtggg cgtttcataa
     2101 agaactacct gctggaacgg ggtgttgcct cccggacgcc atactttcag tccaggcatt
     2161 ccaagctctt cggccaaggc tgcctccacg tactcgctgc gcgatgagtt tcctgccagc
     2221 agcagcacga ctccctgatc cctccagtcc tctcccgttc cccaactcgt gttgcgaagc
     2281 atactcgaca gtaaccgcgc tccatcgcga attctgtctt tgagatggcc cgtaagacca
     2341 gcatgcaatc cggcgatatc ggttttcagc gaggtcagtg agacttcgga gccgtcgagc
     2401 ctcgcggcgc tgagttcttc gactcgtttc ggtgcgtgac ttcgcttgaa cttgacggct
     2461 tcgagttcca tctcccgctg aaatcgaacc atgttttgcc tgcccgccaa gctacgtttg
     2521 tataggtgcg gcttggaaga caacgtgttg ggaggcacgg tttgcggacg catcatgggc
     2581 acctccttgc tttccatttc gggaagatgc ttgtcgaatt gatgcgtgag ccaaaccatc
     2641 tcgtgcgtga gaaaatctcc gcccaggtgg tcatcacctc ctacttgcaa cgtttcgatg
     2701 accgtggagg ctcccgattc cgccgtttcg gtgtcggagg caggacggaa acggccgcaa
     2761 gcgatgtcga gagtaccgcc gccgaaatcg aagacggcaa atcgcaactc tccgtattta
     2821 ctaattaatt cttcgagtgc agggtcggct gccagttcgg gacatacctc ggccgcgaag
     2881 gcttccggct cactggctcg catcgatacg gtgacctcgt ccgcgctgat cccttccggg
     2941 atgctgagca gaatacccgc tcggatctcc tcttcgagaa gcgcgcgcgt gcgtttgtcg
     3001 aacttcgccg gatgggtgag ccaatagtgc agaaacacat cctgtcccgg gcgattgatg
     3061 gcgcggccca acaggtacgc ataggtgcgg atcaacgtgc gcacgcgagg ctcgtccaaa
     3121 aggaagtccg cctgctgttg cctgtcacga agctgcggag attgatcgag aatcaccacg
     3181 cgctcaggta agcttttcaa ttcaccgacg accgcgttgg ggctttcggc catcttctcg
     3241 taagccgcgt gactggctaa caccacgcgc atcaagttgg gaaatctttt tccacccgac
     3301 gctcgggtca tctcatccca gaggcgttga tggtcttcca ccaacagata cgtcggattc
     3361 tcccaggatt cgtcgttgtc ttttccgccc aagcggagca aagagcggaa tcctttgtgg
     3421 tgaaacgccg ccacggtcga ggtcgtaccg aagtcgattc cgacggcact caggcgtttg
     3481 aaacgtttga gatggggaac aggatcgcga agtcgaagag gtacgggagc atgccatgtc
     3541 atcacgcatc ggtgatccgc gaaagaaagt tcatgttcca cgatcacatc tagcggcaca
     3601 tcgcgccctt cgggtggaat gagtccgata tcgtcatggg agctgtcgaa aatgaattcc
     3661 gtgctggtgt gtcgtccgcc cgcgcgtcgc gtggtgtctt cccacaacac atcggagatg
     3721 tcgttggttc gctgccaacg atgaagcaaa cgggaagggc gcgtttcgag ttggtaagcg
     3781 tcggcatcgt cgcgattcca ttcggccatc aaacgcaacc gccacgcacc agttcccacc
     3841 cacaatccct cctcgggatt ctgcaagtcg aaaagatgtc gttccggtgc atcgaaaagc
     3901 agcgcttcgg tggcttcggc aagatcgaga ttggagatgt ggggacccgc ggcgcgcatg
     3961 ggaaatagcg tggtgcgaag caaacgcagc acacgctctc cgggggagat ggccaccccc
     4021 aatcgaatgc tcgcaccgat gaaaggcgcc aacgcgcgca gcggcacgac gacttcgaaa
     4081 gagaagtgca agtaggcgga ggaagactgc cccacgtgcg ccgcaacctg cgaagtctga
     4141 cggttgccat ccacatcgag gctcacctcg aaaggaccgt cgcaaagccc cgaagcccgc
     4201 acgaccaact caccgccctg catccaaccg tcgaaaatcg gaagaaccac gttttccgcg
     4261 acggtcatat ggcgtgatgg tttcaattca tagcggatgc cgtcggaaga gatgcgacgt
     4321 atcaggatgg cgagatcatc gatgcggacc cttggagaat attgacccgt agacgatcgc
     4381 tgaacatggg cggtcggatc ggtcgagctg cttgccatcg attgtgctcc tgcctcctgt
     4441 ggaccccagc ggaggcgctg cgaccgtagc acaacctggc tgatgggact gaggcgtttt
     4501 cgtgcaatcc gtcgggatgg cattctatcg ccaagccctt gtacgatttc ggagcaccgt
     4561 cgaatacctg ggtggtgtta tgcttccagg tctcgcgaaa catggctgaa ctcgtcgctg
     4621 tcattgtctc ttttcccgcg gttgttccta ccgtgctgct cggcgtggtc ctgttgtatt
     4681 gggtcatggt cgttgttggc gttctggatg tggatttcat gagtggggaa ggagcggcgg
     4741 acggtgtttt ggaaggcgtg cagggggtag acgcgttggt gcaaggggcg ccggatgcag
     4801 cggtggaggg tatcgtcgat ggagccgccc acggcatcac ggagggagtt gccgatgggg
     4861 ttgtggaagg gagcgtcgac ggtgtcaaag ggctcgtgga aatcacgcat gcttccgcca
     4921 caccgatggt cacgcaaggc gtggaaaccg taaacgtgct gtcttttgcc cacttccgtt
     4981 cggtgcccgt gacggtatcc gcaagcgtcg taaccgtatt ttcatggctt ttttgtgtgc
     5041 tgagcgtacg gattctctcc ccgtttttgg ggcaaccctc ctgggtgccc ggcctgggga
     5101 tgctgctagc ttccgtcgtc ctcgcgctgc caccgtcggc gctgcttctt cgcccgttag
     5161 ccaacgtgtt tcgggttcag catgccaagt cgtttcaaga tttggtgggg cacgtatgcg
     5221 tggtgaccac gggaaccgtg aacgacaaat tcggtcaagc ccgtctggaa gtgaccgatc
     5281 tcgatctgtt ggtgcaggta aggacggaac aagtgcgtct tcaaagaggg gatcgaggtc
     5341 tcatcgtggc ttgggatgca cgtcgtgagg tatttatcat agagccttac gacaaactgc
     5401 tcgaaggctg atacgcacag cggtgtggcg ttcgggcacg attgttgttg gttctagggg
     5461 gtcctctatg agatcctctc aacctcacca acctggggca attgccatga gtgacctcat
     5521 tccgatcatc attgccattg ttgcgctgtt gcttttggcg ctgggagccg ccgccatcat
     5581 tgcgcgcttt taccgcaagg tattgcaggg gcaggcgctc atcgtcaaca cgatgaaaag
     5641 cgaaccgctc gtgacgttta cgggcgcggt ggtgtttccc atcatacatc gcgccgaggt
     5701 gatggatatt tccgtcaaga ccattgagat cgaccggcgt ggcaaagagg ggctcatctg
     5761 ccaagacaac atccgcgccg acatcaacgt tacgtttttc gttcgcgtca acaagacgcg
     5821 cgatgacgtt ctcaaggtgg cgcaatcgat aggatgtgtt cgtgcttccg atcatgaaac
     5881 gttgcgcgag ttattcagcg cgaagttttc cgaggcgttg aagaccgtgg gaaagcactt
     5941 cgagttcgtc gatttgtata ccaagcgcga agaattcaag gatcagatca ttcgtgtcat
     6001 tggcacggat ctcaatgggt atgtgttgga cgattgtgcc atcgattatc tcgagcaaac
     6061 gcctgtggaa gcgctggaca aagacaacat catggatgcg gaaggcattc ggaaaatcac
     6121 ggcgcaaact acagaaaaga acattcaaac caatgagctt cgtcaaaaag aacgtatgga
     6181 aatgggcagt cagaatttga ccgcggacga ggcgatcttc cgattcgaac agcgtcgagc
     6241 ggaagcggag gccaagaaag ccaaggaaat agcgatcgcc gaggcgcgag aacagaacga
     6301 ggccgctcgt gtggcgagtg acgagggcaa gaaaacggct gttgtccgct acaagaatga
     6361 ggaagaggcg ttggtggcgg ccgaggcgaa agctcgagcg gtggccgtag cagagaagaa
     6421 tcgagaacgt gaagtagcca ttgaaaccga gcgggtggag aaggctcgac aacttgaaat
     6481 catatcgcgt gagcgcgagg tggagcttac gcgaatcgcc aaggacaaag atgtcgaggt
     6541 tcagaaaaaa gagattgccg atgtggtacg aagccgcgta gccgtcgaca aaaccgttgc
     6601 tcaggaagag gagtacatca aagacctccg tgtcgtggct caggccaagc gggacaagga
     6661 agccattacg atcgcggcgg aggccgaggc ccaggaggct ctcgtcaagc aagtgaaggc
     6721 ggccgaggca gcggagcaat gcgcgcgttt caaagccaaa gaaaaactcg cgattgccga
     6781 tgccgacctc gaagcttcgg acaagcaggc gagagcgaag attcgccttg cggaaggggt
     6841 taccgcggag cgttcggcgg aaggtctagc atccgttcgc gtcaaggaag ccgatgcggt
     6901 cgccaccgag aaactcggtt tggtcgatgc aaaaatcaca ttggaaaaga tgcaggctac
     6961 ggccgtgggc gaagagaagc aggggcttgc tcgcgtgcgc atccaggagg cggaggcgga
     7021 agcgattcaa aaacaagggc ttgccaaggc caacgtggtc aaagaaacca agcttgccga
     7081 agcggttggg gaagagcaaa aagggctggc ggaggctcga gtcagggaag cggaagccgt
     7141 ggccattgca aaacgtggcg aagccgaagc caccgccgta cgagaacgac tcgcggcgga
     7201 agcctcgggt ctgtccgaaa aagccaatgc catgaaagcg ctcgacgaag cgggtcgagg
     7261 ccacgaagag ttccgtctgc gcctcgaaaa agaaaaaatc gtcgaaatgc aaagcatcga
     7321 aatgcgtcgc cagatcgccg aagcccaagc gagcgttctg gccgaagcct tcaaacaagc
     7381 caaaatcaat atcgtgggag gcgatggtgc cttcttcgat cgtttcgtca aagccgccac
     7441 cttgtcgcat accctcgatg gcgccgtcga tggttccgat accatcaaga ccgtctttcg
     7501 agagtatctt tccggcgaaa agagccttcc ggaggacatt cggcaaattc tcacacgccc
     7561 agccctgtcc tcgggagatg tgcagcgtct atccttatcc ggattgctca tgcgtctcgc
     7621 gtccggggcc gatgacaagg cccgcgcaaa catccataag ctgttggacg aggccaaaaa
     7681 gctaggcatc gacgacgaat tgattcgttg agccgcggga ggccgtgatg gacgagtccg
     7741 gtgcaaaaaa caccgatggt gcgcctacgc tcgaaggcag tagttacgat gtcattcgcc
     7801 gtcggcttct cgaacaggcc tcggaactac gttcgcgaac ggattcgctg aatgacaaac
     7861 gcatgcgtgt gttcggtagt tccgagctga agctgctgtc caatgagcgc gttcgaaccg
     7921 aaaacaacag cacgccacgc gacatggtca gcatctccgg ccatctgttg tttggctttc
     7981 aggtctttct cggcctaaaa agcgaaacga atgtcgccga catcctcact ctgtacaagg
     8041 tgtctcccac gagcgaaggc ttcgatctga ctcccacggg cctggacgcc gtcggcggtt
     8101 ttcttgcctc ggaggacttt caaaaagagt tccgagatgc ctttcggtat tccaaagagg
     8161 cggaacttct tcagatccga cgcaccgacc aacggctgct gctcatcgtt caggtgggca
     8221 gcacggtctc ggatgttaaa gtattccgtt ttagcatcga tgccgcaggg cgagttcgct
     8281 acctcgacgc tcgtggcgag gaagatgccg tattgcctcg cccccatgat ttcgcctgga
     8341 aagtgaccac acgggaggat caagtcccgg gagcccatcc tcatgtctcg atcgaggacg
     8401 ctgttttcgt cgaaaccgta ggaggggatc ttacggtaaa aattgaggat aacacgcggg
     8461 atgggtgggg tatttaccgc gagccggtcg acgatccgaa tcagtcgctg gacgacgctt
     8521 cgattgcgta tgcgcgattg ggggcgttga ttgttcttcg cgtcaaaccg tttcgtgagg
     8581 aagcgtatcg ttacctcgtg ttcaatacgc gaacgaaagg tgtggcgcgt atcgatgcca
     8641 tcgaacaagc gtgcttacaa cttccggaag agcacggcat catctttccc ggtgggtatt
     8701 gtcttcgaac gggtgaaacg aagcggttca tcgaagaccc tcgtggcttg cagtatttat
     8761 catcgattcg gtcgccgaac ggtgaggacg tgctttacgt ttttcaaagg cccaccgatg
     8821 gcttctatca cctgatgccg tataacctcg tgcgcaagga ggtagccaat ccgatcgcgt
     8881 gcaacggatt cagtctattc gacgatggca agatgatcgt gtttcgcgcc gctgcggagc
     8941 cttcgcgggt ccatgtggtg caagtatggc aaacgccgtt tacgagcgcg gaattcgccg
     9001 ctaccgcacc caccgatggg tcgtttctcg gcaaagtggg caatgcggac ctggtgagag
     9061 gaatcagcga cgcttatacc ttggtcacat tggcgcgcta cgatacgcct tcccgcgcat
     9121 cgtatgagga gttgatcgcg gcctgcgctc ggttcgagga tgcgtacttt tgggggaacc
     9181 acgcggaagc gggtaacttg aaagaagccg tttcggcgat gcggggcacg acggaactca
     9241 tcatcgatga gtttgagaaa gtcgccgcga ttcgacgtcg tgcctccgag gctttgggcc
     9301 gtgccgccga cgaacagcga gacctcgtca atgaaatcat ggcttcggac ttgtcgagcc
     9361 tcgatgcgta tatgggggcg ctgacgaatc tgcgaaagca gcgtggcaaa ctcatctcgt
     9421 tgcgggagat gcgcgagatg gatcttgtca cggtcgaagc cttggagaac gaggtggtgg
     9481 aacaattcga ccgcctgagc caacgctgtg cggagtttct ccttgggggg gaggcgtttg
     9541 cccccttgac gactcggatt gaggagcttc aacaacaaat cgaggcggtc tcgaaagcgg
     9601 tcgaactcaa cgccctcggt gccgacctgc ttggagtaca agaagggctc acgcttctat
     9661 cggagatcgc tggaggattg gtggtcgagg atgccacggc gcgaacgcgt attctcgagg
     9721 cgatatccga agtattcggc caggtaaacc gtgtaagagc cacgtttcag gctcgacaac
     9781 gggacttgtc cggggcggag gctagaacgg aatttggggc gcagtttgct ctgttctcgc
     9841 agagtgtggc aggagcattg gccgtctgtg attcgcccga acgatgcgat gagcagcttt
     9901 cgcgcatgct cgtatttctc gaggatctcg aagggcgttt cggcgagttc gacgagtttc
     9961 tctccgatct cacgatgaag cgtgaggagg tcacggatgc gtttggggcg cgacgtcaga
    10021 cgttggtgga cgagcgacag cgcaaggcgc agtcattgtt ttccgcggcg gagagaatcc
    10081 tgaccggtgt gatacggcgc acgggaaaaa tggatagcgc ggatgaactc aacgcatatt
    10141 ttgcttccga tccgatggtg cataagttgg gtgatttggc tgcccagctc gatgcgcttg
    10201 gagatacggt caaggctgag gagctgcgtg gacgtttgat ggcggcgcgt caggacgccg
    10261 tgcgagcgca acgtgatcgg tcggatttat tcgaggctgg aacggaaatc attcgcctgg
    10321 gcaatcatcg attcagtgtc aacacgcaaa gtttggaagc gacgttgttg cctcgtgatg
    10381 ggacgatgac gctgcacctg accggcacgg gattttatga tccggtgcga gacgatgcgt
    10441 tgctggaggc gaaggatctt tgggaacagt cgttgatttc ggaaagcccc gaggtgtatc
    10501 ggggagagta tctggcgggg tcgttgttgc ttgcggccga ggaggggcgg gatggcctgt
    10561 cgatgcgaat ccttcaagat gcctcgttgc aagaaggcgg gctatccaaa tgggtgcggg
    10621 agctagcggc caagcgctat gacgagggat atgagcgggg catccatgat gaggatgccg
    10681 cgcggatttt ggaaaaattg ctcgcgatgc gggcaaccgc gggactattg cgcttttcgc
    10741 caaaaccgag agccctggcg tgtttggtgt gggcctattt gccacgcgat gaccgaacct
    10801 tgttgcatcg ccgcgcgcgt agctacggaa ttttgcgaga caagctggac gatgagggag
    10861 cgcaatcgtc tcttggcgaa cagctcgaag cgaccgtacg tggcaccgct gagtcatttc
    10921 gtatcgaatg cagcgtttcg gatattcgtg cggcaagtcg ttatctcgtc ctggaactgg
    10981 ggcaggatca cgtacggttt tccacgagtt ctgaagcgat cgccgtgaaa gacgcgttgc
    11041 tttcgcacct cgatgatctt ggagcgcgcc gcgctttcga agaagacctt cgtgctctcg
    11101 atgccttcgc ggcgcagcgc ttggaacttg cacaaaacta tctgaaagct ttcgccaaaa
    11161 gacagagcga gcgaaccatc gacgatgggc ttgccttgga agccgcggtg ttgctcattt
    11221 ccgaacgtct gctcgagcgt caatccgtag ccgccgtgac ctctgtggtt gttaccgaac
    11281 ttctggggca acatccgaga attcgcgaac gctcgatgtc ccttcgattg gatgaatttc
    11341 tcgatcgttt gggtgtgttt gtgtacgaac gagtacctcg gtttcgcaaa ctcagggagg
    11401 ttcgctctgc ggtcgtcgcg agagagcaac aacgccttcg cctcgatgag tacttgccac
    11461 gggttctgag ttccttcgtt cgcaatcgtc tggtcgatga ggtgtactta ccgctgattg
    11521 ggacgaattt ggccaaacaa ctcggtgctg tgggggccgc gaagcgaacc gattcgatgg
    11581 ggttgttgct tctcatctcc ccacctggat acggcaaaac cacgttgatg gaatacgtgg
    11641 caagcaaact cgggctggtg ttcgtcaagg tcaatggccc gtcgattggt catgcagtga
    11701 catcgttgga tccggcggaa gcacccaatc ttacggccaa gcaagaggtc gaaaaaatca
    11761 gttttgccct cgagatgggg aacaacgtga tgctctatct cgatgatatc cagcatacga
    11821 atccggagtt gctgcagaag ttcatctcct tatgcgatgc gcagcgccgt atggaggggg
    11881 tgtggcgcgg acgaacgtgt acgtatgatt tgcgaggaaa aagattttgc gtggtgatgg
    11941 cgggcaatcc ttatacggaa gcgggaacga agtttcagat tccggacatg ctcgcgaacc
    12001 gagcggatac ctataacttg ggtgaggtat tggaagggaa ggctgaccta tttgcgtcga
    12061 gctatctgga aaacgccttg acttccaacc ctgtgcttgc gccgctttct gggcgtgcgc
    12121 ccgcggatgt ccacctgctc gttcgtcttg cgcgcggaga ggaggtgcag acgacccacc
    12181 tgtcgtatgg gtattcggaa gcagagattc aggagatggt gtcggtattt cgtttgctca
    12241 tacgtgttca ggatgtgttg ctgaaggtga accagcagta catcgtatcc gcggcgcaag
    12301 aggatgcgtt tcgtacggag ccgcgttttc aactgcaagg cagttatcga aacatggcca
    12361 aaatcgccga aaaaatagtc ccggcgatga atctccagga gttggatcgt ctgatttcgg
    12421 atcactacat ggccgaggcg caaaccttga ccgcgggagc cgaggcgaat ctgctcaagc
    12481 ttggggagct gcgcggaacc ctgtccgata ccgagcggga acgatggaat gcgatcaagc
    12541 tcgagtatca gcgggtcaag cggatgggag ggcgagaaga cgatccggta tcccgggtga
    12601 caggtacgat cagcggtttg gatgaacggc ttgcgagcat acgggagacc atcctggagg
    12661 cggcccggga ggcaaaaaaa gctagtaaat ccaatgcctt tgatgcctcc gggggggcga
    12721 ttctcccgct cttgtccagc ctggaaaaag gatttcgaga actgtcccaa cccaaggtat
    12781 ccctgcaagt gcagcagcag cctcctccag ggtttgggga tttgttggcg caacagatct
    12841 cactcattca aaacactctc gtgccgctcg tgcaaacggc aatgaatcgg ggggggcgtt
    12901 ccgaattgct cgaagcacgc gtcgccgaat tgacggcggt cgtcagggag ttggcgtcgc
    12961 aaagggcgcc ggatctgtct ccaccaccac gtttcgaggt cgaggtgacc ttggacggcc
    13021 ctagcaattt ctatgtggac aatccgcagg caggggtgtt gaaacagggc ggcctatttg
    13081 tcgccacggt ggtcaagcct cccgcggtcg gacaacgggt ggtcgtcaat gtcgtgtttc
    13141 cttggaaaca agagcatgac atcgcgggtc gagtggactg gatccaatcg caaacccctg
    13201 ctgccgacga tgccttaagg cagggatttg gcgtgaaatt ggaaaacatc gatgccaaca
    13261 cgcgcgtatc cattctctcg ttcgtgaagc agcgtccgcc atgggtactc acgcgctaat
    13321 cacgcgatga gttcgtcgcg gaagaactga gagaacggct ttttggctgg cttgggggct
    13381 cgtgagcgac ggtaaggcgt tgcggccgac agaggtgggc atgaacgcac aatccgtatt
    13441 cggatagttt ctgcggctca ccggcgtgtg gttgaaatac gagtattgga taattacttg
    13501 cgtcgttgga caaaccacag cccggtgatg agaagtgccc accaagccat cgttcggtgt
    13561 tgcgagggca ctgtcctaca gccacaacca tcatccccgc catcggaatc aaaagcggca
    13621 ccggtaggcg gtgcggtctc tggctggttg gtgtcgggta ctgacccagc ttccaaactt
    13681 ggctcggggc cagcttcggg tgtgggctct gggccgggtt cctcactggg ttcgggttcg
    13741 ggatcgggct tgggttgcgg ttgcacatcg aacgtcacgt gcaatgtgct cgaggcgggg
    13801 ccgccgtaga cgcgaatctt ggcttcatgc ttgcccacgt tcaacgattc ggcgtttgct
    13861 tcgatcgtca acggcgatgt ttggccgact ccgaggtttg tgttcttgag cgtggccgtc
    13921 atccaggaag gaacggaatc gatgccgaca tcgaccgtcc aggacttacc gcagttggtg
    13981 atttgaacac ttccggaagc cttttcaccg acttttgcct gcaagtgcag gctgttgtct
    14041 ttcaggcaag gcatgccggg tgtttctgcg gggtcgacat cgaggtagtt cacgtgcaga
    14101 gcaaaacaaa tcccttgcaa ggtgagggag cagtctgtgt ctcgcgtgat tttgaaatag
    14161 ccgccgtctc cccatttcga cccccagcta ttgcgaacga tccaactatt gtcgctgtca
    14221 tcccacccga cgatcgtaac tacgtgccaa ccatcgggtt tggcgctgct cgaccgcgaa
    14281 tacacacccg acttatatcc ataaaaatcg gagtagattt gcatttggga gatcacagga
    14341 gttttgacga gctgcttttt gatctccgga accgaccaag ggatgatgct catatgccag
    14401 tcgtcgatgc gtacgcgacg ctctttccaa tcgctgcaga cgttggagca ttgtccttcc
    14461 tcggcaagat agggataaca ggcttcgtcg ggaaccccat gggttttcaa gcgattgccg
    14521 acttcttccg cggttcctcc gttggcgcag cttccgaggg tgatgcagga gatgacgctt
    14581 tgctcagata gatcgacatc gagccatgga ttcgcggcga tgactttgag ctggctttcg
    14641 agcgctccga gcgtaccaaa tacaaagcag cttccgcagc ttttttgatc ccgaacgggt
    14701 gacatccaat cgtcgccacc gacattatgc caattgaatt tggggtcggt agggacagcg
    14761 ttgggtaagt cgcggagctg gggcggcggt gtggaggagg tgggaaagga gggggcgggc
    14821 atgagccttg cggcacgttg tgccgatgac atttgcgtca actcattctc gcaccataag
    14881 aaagaggaag aggttccttg aacgacccgg catgcgttcg tttcggcgcg tgcggtgaag
    14941 gaatagagcg ataaagcgat ggtggggagg ctaacgagca gagtcgattt gcgcatggtg
    15001 gctttccggt gacaataacg ccaatactag gggaagtaac cgcatatcga tgccaaggca
    15061 agccacacga agggggcggt cgtgagcgga aggaaggggt tgtgttggtt acgaattaga
    15121 agaagatacc gccaaaccag cgcaaggtat gggcggcgga ggtttcgagg cgaatgttct
    15181 ggttggcggc ttgttcttct tcgccgggaa gatagccgag ccctcccaga ggaaagagca
    15241 caccgtattg tagcatcgtg aaaaaaccac ccatcttttc cggatcgtcg ttgagcgaac
    15301 cgtctttcga ctggaagtaa agctgcaggt cgatttcgat gcccagatcg cgtcggtgtc
    15361 ccggggcttg gacgaattcg ctcgcgcggc tccagatgac agcgccccca ccgccgaatc
    15421 gttgtccatt gggtttgcgc gagaagtcac aatcgacgga aggtcggaag taataggtgc
    15481 cttgtacgcg attcaaaata tgtcgatgca agatgagatc gacgcggtag tcggggtgaa
    15541 agcggaacgt tgaatccgtt cgatcatcgg tgcgttgtgg ttgtagaccg ccggagcctg
    15601 gcgtcaggcc gccgacctgc ggtccggtga cgtcggggtc tcccgaagcc catccgaagc
    15661 cgaattgcag gctcaatcgg tcttcgaccg ttcggaattc ggtttgggtg gcgacgccat
    15721 actggcgaat tttatgtccg ttttcgtctt ctcgaacgtt ttcgtagttg tttccagcta
    15781 cctcggtgtt gtcgagggag ccgcgaacca tcacggcttc cgcttcgaat cgcagttttt
    15841 tgtataagat ctgcagccac aaatcgggga tgatgatttt ggcattgcga cgtacatagc
    15901 catcctggat ggtgtcggct gatgccccca agttggcggc gttggtggtg ttgaaagcat
    15961 cgttggccaa gaattgatgc cgatacgtaa actgcacgcc accgttgagg accaggcctc
    16021 cccgtgacaa cgtaaggcgt tgcaacgcgg gctgtttacg acgcatcagc gtcagaacgt
    16081 actgattgac gtcatcgagc tgcgcaaggt cataggcttg gccttgctgc tgagcggatt
    16141 ggctgatggc accttcgcca ggaaagtccc acgctgcgct gacgtacaga tcgagcgctt
    16201 tgatgcctgt cgtgaacatc aaccggtcga cggtggattg cgtgtcggag tcgtagccat
    16261 cgccggcgtt ttgcaccatt cccaagcccc aatgagaagg catacgaccg aatcgcagtt
    16321 gtccgatggg ggtcaaatac tcgccccaca cgcgtttgac ggtgatggaa ttaccgtagg
    16381 cgttataacc tgcgacaggg ggcatcatgg tttcgttgta gacgccgaga ggggtgtagc
    16441 cggagggcgc gacgcgggcg aagccgccgg acgatgaggg ctcgttggca tagccggtgg
    16501 gagtcgatcc aagcacgagg ttatcgagca agtcgatctg cgacatgatc cgaaggttgt
    16561 cgcttacgtg cagttctgga ttgagtcgga aacgaatgtt ggcgccagct tgcgttttat
    16621 ccgtgcaata ctggggattg gcagcatctt cgccacaagc gtgggccgcg tgttgctgaa
    16681 tctgaccgtc aaaccaactc gtatagctgt ggtcgggggg gcgaggccaa agggctttgt
    16741 cgtacggatc cgaacggccg aggctgaagt tttgaaatag ttccgcgcgc attcggaaat
    16801 agccatgaag ctcgagaatg ggacgcgcga tatgccacca atcctcggcg aaaacttgtt
    16861 catgattttc gacgacgggt gcgggagcat cgtgttgcga tatcagtgac gacgaagagg
    16921 cagcgtttgc ttgcgatatt ggggcagggg tgagggaggg gtgcgcgttg ggcggggact
    16981 ccgaacgacg cgtttcggtg gacgcgggtt ccggtgtctc cgaggattcg tcttgggagg
    17041 ccgaagtcgc gtcgtcggag gtttccaatt caccttcggg ctttggttgt gccgaaaggg
    17101 gaaaggaaag caagagcatg ggaacgacaa agacggcggc acgtcgaatg tgcggcatga
    17161 aagagattct ccttcaggac tgtgagcggg gggaccttgc ccttcgttgg cgtcgcaagc
    17221 aagccccata tcacgagacc cggcacgaag gggtcgagaa gcctgaaaca agcggtaggc
    17281 gcgaacccga ggaaggacca aaggggtcac ttggcgtaga cattggcgag atgattgcgc
    17341 atggcgttcg catccacctg ggcaatcggt tgtgttccac ctttttttcg gggaggaacc
    17401 atagcagcgg gaaccatgcc ggtggcgcgt ttggcaaatt cacgttgggc tcgccagcca
    17461 ggggtttccg gaatccccag ggcttggcaa aaagcgttgc ccaactcaat agcgcttggg
    17521 tatcggtggt ccggattttt ggcgagtgcc cgcgcgaaca atagatccat gaccggaggc
    17581 gcttgcggaa gcaattgggt gatcggcggc ggcgtgtctt cgacttgcga tgctcggacc
    17641 atcaattcgc tcttgtcacc ctcgtcgaag ggcgtcttgc ccgtaagcat ttcgaataag
    17701 acgatggcgg cactgtacaa gtcactacgc ccatcgagtt ctcgccccaa cacttgttcg
    17761 ggagacatat aagctccagt accgggttga acgcccccgc tgcgacgcgc ctcatcggcg
    17821 ggaagccggg cgataccgta atcggtaagc ttggctaacc cgtccctacg tatcaacaag
    17881 ttggcgggtt tcacatcgcg gtgaacgatg ccaagttcgt gggttgccgc aagagcgccc
    17941 agcaattgtt cgaagtaatg ccaagctcgt tgaaaaggca ggcagggctg acgataggcg
    18001 cgagcggttg gtgcgtggcg agcgatgaca ttgtccaagg cttcgccatc gacgaattcg
    18061 atgacgagag aaagctgccc cggagcttct acgagcgaga aaaaatgcac gatattcggg
    18121 tggttgagac gttgcagggc ggtggcttcc cccacgaaca gctttcgaac gaagtcgcgt
    18181 gtggtcatga gggggtggag gatttttata gcgacggggt gcgatggggt attggcttgt
    18241 gggccatggg ggttgtagta catccacccc tgataaacga cacccattcc gccatccccg
    18301 atgaggtttc ctacgatgac ttggctccaa ccgaggtcta ggcgggcacc agggccggtg
    18361 gtgggagcct ggatgcgagc gggggctggg gctccgcaat tcgggcagaa tcggccgtcg
    18421 ttcgaaaaca ctgtcttgca accagcgcag accgtcacgg cccagagcat atcaggttgt
    18481 tggctgtcgc gccgaccttg atgcgaattc gccctatgac gtccagatca gttgatcgac
    18541 tgggcccctt cgtgcgagcc tgtggttcgc cttggtcggg attgcgttcg gcctgggcag
    18601 ggaagtgacg gctgttgaat ccaaggagaa atcagtaaat gagactgcgc cctctcatga
    18661 tgtcatgcgt gctggggacg ggcatgatgc tggctgctgg agttgcttcg gctcagcaag
    18721 aaggtgagtt ttccgttcaa cgcttcgagc ctgcccctgg accgaataac ttcgtaactg
    18781 tcgaaggtgc tcgtgtcacg ggggatatgg cttggtccgt tgggctgttc ggaaactacg
    18841 ctcatcgacc gttcgtggtt cgtagttgta tttcggaaac gaattgtgat gaagccaaca
    18901 agttgttgaa cgacgacatt gcggttgtgc gtgacctcat tacggcagac gtgctggcgt
    18961 cattggctcc gatatcgaga ttgcaaatcg gattgcgtat cccgctgtcg tacgtcaatg
    19021 gcgatggtgt cgatacggtt cgaggccagc cggcgacggg tgggctatct ggctttggtc
    19081 ttggcgaccc cacgttggag ggcaagtatc gattctttgg tgagccggac acgccgttgg
    19141 ttctcggtgc gggtgcgttt gtttccggcc ccgtgggtca cgccatggcg gaaggtaaat
    19201 acatcgggta ttcctcgccg gtggtaggac tgcgtggaat cgcggatttc caggcaggag
    19261 ccttgtctct cggcggaaat ctcgccgccc tttatcgatc cagtgcgacg ttgggttcga
    19321 cggaactcgg acctgaattc cgttatggtg gtgcggtggg ctacaacgtc agcccggtgt
    19381 tcaaactggt gggggaggga tacgggggaa cgaaattcag cacaaaaaat ggaaccaata
    19441 gcttggaggc gttgggggcg gtgcagatca tgcccttgcg ctccggcttt tcgttcacct
    19501 tgggcggcgg cgccggcgtc attcaaggcg tcggggttcc gatgtttcgt ggattcgcag
    19561 gtattttgta ttctcatacc gccacggata ccgatggcga tggaatcccg gatgatgtcg
    19621 atcagtgccc gactgagccc gaggatttgg acggtttcga ggatgaagac ggatgtccgg
    19681 atccggataa cgatggagac accatccccg acgatcggga caagtgcccc aatcagcctg
    19741 aaaccttcaa tgactaccaa gacgatgacg gatgtccgga tgagattcct gaccgggaca
    19801 aagacggcat agcggatagc gaagacaagt gtccagacgc gggtggcgaa gtcattcgtg
    19861 tcaagggacc tcattatggt tgtcccgacc gtgacaagga tggcatcccg gaccacttgg
    19921 ataagtgccc cgatgagccg gaggatacgg acggctacat ggacgaggac ggatgtccgg
    19981 atccggataa tgatggcgac ggaatcccgg atgtggagga ccaatgcgtc gacgtgccgg
    20041 ggcccaaaga caaccacggt tgtccttacg aagacaaaga caaggacggg atccccgatc
    20101 acctggataa gtgtccagac gaaccggaga actacaatgg ataccaggat gaggacggtt
    20161 gtccggacaa taggcccacc ttggtgacgc aaacggcgga cgccatcgag attcagggct
    20221 cggtggagtt cgccaccaac agctccaaaa tcgtgggagc gaaaagcttc cagatccttg
    20281 atggagttgc ttcgctcatg gttcacaacc tgcgcatcca acaggtggaa gtacaaggtc
    20341 atacggacaa tcgaggtggt gaagccgcca acaagaaact gtcccaagac cgagcggaag
    20401 ccgtcgtcaa gtacctcaca gaaaaaggcg tccaggcttc ccgactcacc gctgtcggat
    20461 atggtcaaga aaaaccgatc gcggacaaca acaccaacgc tggccgtcaa aagaaccgac
    20521 gggtcgaatt caagatcacc cgtcaagcca agtaaccctg cctcttctct tttcctcacc
    20581 gcgtgtgcta cccattgccc caatgatcgg ggttcatctc tctcgtcacc tccagcctac
    20641 cgtccttgcc gcagggctgc gtctagcgca taagccactc gtgatgggcc tggttgcctg
    20701 ttgcctggct ctcacttcgg aatctttcgc ccaacagacg gattatcaag gtccatctcg
    20761 cgcgcctcac gtccggctgc tcctcgatgc acacacgtgg catggtccgt ggacgatcac
    20821 gttcgtcaac gaagaagacg ttcccgtccg tattgccgcc gatgctcgtc agctctccct
    20881 tctcgttcgc cttcctaacg acaaaaacta caccaagtgt agccttccct catcgatgcg
    20941 cgggaccggg cgtgatcgac aactcgtgct cgaccccgcg caatcctatg tcgaaacgat
    21001 cgacccaagg ctgtactgct ggggagaaaa tcaaacaaaa ctagctccag gggcttccgt
    21061 gactgcgttc gtgggctggg ctcctgatgt gcgtttggaa aaacggtcga aaccgcaagt
    21121 ggctcccttc gtggtggagc ctgttctggc tccaccagcc tttccgttca ccaaacagat
    21181 ggcttctctt acccattggc tcgcagcgtc agacgattcg ttgatcgcgc agcccgagca
    21241 acctcctgcg cggtatgtgg gaacaccgga ccttcggctg agcacaccga agtgggaaga
    21301 tgcggacctt cgctccgcgc gtatatcggc gacactcacc aatgttgggg accgtgcggc
    21361 gctcattcat attcgtcccg atgacctgga attgcgcgta cagcgcccgg atggatcgat
    21421 ggccgcgtgt ggaccggggt ccagctatcg cgcggccgtg cgtgatttct ttctcaccat
    21481 tcaacctggt aaatccgtgt cggtcagctc tttgctttct gagcgttgtc cctccggaac
    21541 gttcgagcga cccgggttgt atcaattgca caccacactg cgcgcccgtg acaatggttc
    21601 ggcctttcac ttgcaagcca tcacgggtga cttcgcggct cccacaccaa cgctattgcg
    21661 gcttcgaacg gcgcgattgc cgttttgccg ccgtccccga agcctccccc gcgactcctc
    21721 cacgtaacgg accgtgatag tgtcaaagag atgatct
//
LOCUS       DAOSOR010000024        21640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_2417_length_21640_cov_4.001899, whole genome shotgun sequence.
ACCESSION   DAOSOR010000024 DAOSOR010000000
VERSION     DAOSOR010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 21640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21640
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2417_length_21640_cov_4.001899"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PK710_02385"
     CDS             complement(<1..245)
                     /locus_tag="PK710_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08584.1"
                     /translation="MLSPRTLTVACFLLGLPLLPVACGSSEDNSASSGSGGKPSDAGS
                     EDGTGGTGGAVGGSGGTGGGVLIDASNGCKSAADCDGG"
     gene            complement(390..1346)
                     /gene="trxB"
                     /locus_tag="PK710_02390"
     CDS             complement(390..1346)
                     /gene="trxB"
                     /locus_tag="PK710_02390"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOT08585.1"
                     /translation="MSSQIHNTIIVGSGPAGLTAAIYAARANLQPLCIEGFSAGGLIP
                     GGQLMFTTDVENFPGFPEKIQGSELMQRFRDQAEKQGTTFITADVTKLDLSERPFKVW
                     TDEEQPYLAKTLILATGASANYLGLDSEQKLKNRGVSACAVCDGALYRNKDVAVVGGG
                     DTALEEATYLAGLCKSVTLIHRRDEFRASQAMQKRLLDNPKVRVLYSHVVVDVLDPAK
                     GEVTGLKVKNLKTGEVSTLDVSAMFAAIGHTPNTGLVQGQLELHENKYVKTKPGTTQT
                     SVPGVFAAGDVQDFVFRQAVTAAGTGCMAALEAERFLAAHES"
     gene            1528..1995
                     /gene="ssb"
                     /locus_tag="PK710_02395"
     CDS             1528..1995
                     /gene="ssb"
                     /locus_tag="PK710_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOT08586.1"
                     /translation="MAEGLNRVILLGNLGADPELKVTQGGASVLKLRLATTEKYVDRD
                     GAKQERTEWHAITIWGKRGEALNRFLTKGSRILVEGSLRTTSYEDRDGNKRFRTEIVA
                     SNVVFAGGGGAPRSRSSSSEGDFDPPPAPRAASSHDSPPEAPDYSDIDNEIPF"
     gene            2035..3732
                     /locus_tag="PK710_02400"
     CDS             2035..3732
                     /locus_tag="PK710_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08587.1"
                     /translation="MLCLTSLVTFGCDGCKKADNKPQVIHELPEVPAPAHHLADLFVP
                     KPEETYSAFRTHIGGPLALLPSTFPSMVVTSLGLTTQLLEQIDGKSPMVGVMTDDGSR
                     LVVVVGIHVRDGNRTIQLMTEGADAKYSAKPAERGVIVLEPKPTLTSRVATLGVAGHY
                     LMTAEKAQDLVECGPYVSGTLSKRTGIEGQIVVETHQDALKGPLAKRMREAWASFKAA
                     REKEDDKLRKERGRSPDFGEPAAALADVGDKVETIVSVLSDLKRAQLRIDSDAVGIHA
                     LLTMVPASIEGVAAKEFSSLVVGDLAPLLAMPQDTVVGLLVRDDEASRELGAKKHAEG
                     IEKLLGERIKEPENNKVKEAMLQWSKGRGDYLAASLRWTRDEQEAIARGAVRDRAEFE
                     RGIRSVLGLLAIPAIREPVEHHLGKLTLSNVQKVGPGFAMHVDRNKKVPEGQPAQSTS
                     FDVAWRVDDSTFEARVKPDGKAWLTKDEEKRPSLADHPTTSKIFAGLGADASFVLFFD
                     PQLFLSTLVPPSMKAKETNAPFVIAYGGANQQGWFKLALSHASARELIKMLGRQELK"
     gene            3762..4007
                     /locus_tag="PK710_02405"
     CDS             3762..4007
                     /locus_tag="PK710_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08588.1"
                     /translation="MSTEEELKAELDRLRAENEALKNRKAPVVSIKVSAKGGVSVYGL
                     GRFPVTLYKEQWLRLLDMGEEIRAFIQDNDANLKTKS"
     gene            4266..5834
                     /locus_tag="PK710_02410"
     CDS             4266..5834
                     /locus_tag="PK710_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08589.1"
                     /translation="MRTPIHIALMLAVVSGSLLNACGARSPMQEQWADAGVEPDATLD
                     APSDVVYDHVNDINGCNPSTCSGCCDLQGNCRTGTQPSACGQGGYECVDCSSLGQTCI
                     TSTRTCGAVQPCNPSTCPQGCCHNGLCVEGASHEACGSHGMDCVNCASLGQTCNPYSL
                     TCEQSSMCDPTNCAGCCDAWNQCNWGGDPWACGFGGGGCEDCASQGLTCDPFWGGCVT
                     VEPCGPWNCLGCCDMVGNCQWGADDWACGGNGELCQDCSAFNSSCDSQTHTCTGTQLC
                     GPENCKGCCDPYSGQCRMGWDPWACGSAGQICQDCTGSNMICGDSPFGGGQCLSISQT
                     CGPNCKGCCACNTTGCQCLGGTDIKACGHDGQICQDCTIGQGQCIHEPMGNTCISTPP
                     PPTCGPWNCPGCCVDGTPPTCVEGLDAWQCGWGGQFCSVCYPGEACVWQPWGGGQCMA
                     MTDGGPPDGGPADAGMADGFAGCNASNCAGCCGANGQCRAGKSVHQCGRNGELCENCA
                     VKNQVCSDGDCKNP"
     gene            5864..6973
                     /locus_tag="PK710_02415"
     CDS             5864..6973
                     /locus_tag="PK710_02415"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT08590.1"
                     /translation="MVSSLASAVLLTGYPSFLARRMAEQILAHEPNTQLYCVVRSKFL
                     PWAQQTRLTLPASQRNRMVLLEGDAASMDMGLSGAEYREVIEKVDKILHFAQYRDVRA
                     DPQWAHHTNVQGALEALEMAYAAPRLTHLVCMSSIDVLACSGLARESDPPKSPPFRTF
                     IQESLAHAERIYRRAAHRIPVTILRPSTMIGDSNTGEIDRLDGPYPLFMLLVSSGRDT
                     IPLPLRADHLLHFVPVDFVIRSAHIIANSPWSPGKTFHLVDPAPLSARRVFELVARVA
                     ERRSSRGDVPSFVARALVRSPGVESFARSPRAFLDDFSPRARFDATNTEAILQGTGVS
                     CPPFESYVEAIVRYVQGRLHEQREGGVHPMHDPLL"
     gene            6979..8400
                     /locus_tag="PK710_02420"
     CDS             6979..8400
                     /locus_tag="PK710_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOT08591.1"
                     /translation="MLKHGLFRSKAHRQVAVSAFAVVSSAAVVVTCSAVGPRPPKVQE
                     VQEKEQKEPTTPSLVQAAVSSAASQALAEPSPPQVGRWFAPSGLGMTPEGNAVPLLPR
                     FYDALARLETRLRHDHVRIVWLGDSHTQADGWTQAVRRALQQRFGNGGPGFIHLGWTT
                     YGYRRENVILRASGAWSLLPTTLVAVTRVDDGVLGLGGLRLEPRGLDATAQVGVNAAD
                     LPGEGLWDLAVRLMEKDSSLVIRLEGQEHRVEAQSFTLGRIRHVTLRSKGPGGTLGVE
                     QGWNRPQLLGLVVEATDRHGVVLDTLGLNGARYRSALAWDEAAWVGELARREPDLVVL
                     AYGTNESSDIKLKAELHAARVKELVGRVRKAAPHADCLIFGPIDRGGEQYEATVARIN
                     EAQAAAARELGCAFWNGQQAMGGAGSMQRWATMNPPLGGGDRVHLYPRGYQKLGEMLA
                     RDLLTGYDAGVVRGQSPSDDPER"
     gene            8632..9351
                     /locus_tag="PK710_02425"
     CDS             8632..9351
                     /locus_tag="PK710_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08592.1"
                     /translation="MRQFKGFLQASFLGLAGLLVSTGAMAQGPAAPAPTEPDHSSVVG
                     SLGVGYLGASQVAIGVGGTTMTAPVVGVRYWLDPMLGLDLGLGLGMTSGKTKTSPANG
                     PSETEDDPSFTAFIIHAGVPLSLREGKHYSFQIIPEANFGMGTGSAERGEADIDYSGM
                     RFDIGARAGAELHFGFIGVPELSLQGSVGLQFSTASAKTETKQADQKSKVEASATRLG
                     TTVHDNPWNIFTTNVAALYYF"
     gene            9392..10048
                     /locus_tag="PK710_02430"
     CDS             9392..10048
                     /locus_tag="PK710_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08593.1"
                     /translation="MTQPIRNIVAPWAFLMLVACGDGGSGQSGLSLNDGNGEPTSSWT
                     ENPSSNTETPSRSGDSLPSNHESSGSSGETSGGSNGSSEGSATALCKAVCNKLSDLAC
                     TPVSESECLSACAEEYPELISQMGGERCLSEITAVFRDCTFYCDIEGEFALDPDSCPN
                     QLTALYECAGGDFDFDDDVGNDTTEPSCKPPSCMGCEDVCSTCLCSTGDSEICAEYCE
                     "
     gene            complement(10144..11541)
                     /locus_tag="PK710_02435"
     CDS             complement(10144..11541)
                     /locus_tag="PK710_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08594.1"
                     /translation="MSLANVSDPRWQQIGEVRFLGASSRVRLRSAAESSGWLMVNVPP
                     LGPGARGQLTTLIEEAVETALQRRGAPPSGLNVHAESTQIIEDQLYRAQRVGAHGIAV
                     ALESLSGLIGAQGALGDDDSACLRFYSSAASRYPFALWLSSTDVSLRAYGPPLALQDL
                     LGAKAELDTRTILPSTAPALTSTVTSPKDWRHYAKLIDDAHGPKPLSAVEKLFEQAYT
                     PLAEAVRQGENDRRARQSLLQFAEAFEKSYKEAFIAAKVTHKRPTMVLDIPGIASRIG
                     RLHGTRGITLVLVDGMRYDVGLRLEHVLQEELRGTAVLAERVLLWSALPAVTAVQLRL
                     LERGVQALSEPLDSQELREAELPIGRGRTASILRRIRVGGRELHKLDVIQADLTTSGL
                     PEAQRLDALARAVAFPLLQLTSQLPAQTLLFIFGDHGFVLPSHEKGTGPAMAAGARPE
                     EVLVAGQAWVVGSVH"
     gene            11732..14617
                     /locus_tag="PK710_02440"
     CDS             11732..14617
                     /locus_tag="PK710_02440"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOT08595.1"
                     /translation="MSIRHRAPWAIASLIALVGCWDSDGYVRPRPSPTSAPPVEKGPL
                     GNELNVNGTIDLAGLSAPVDIVRDTHGVVHIYAQTHADAFRAQGYAMARDRAAQIELL
                     RRTADGRTAQILGAVSPSTIDQDIVMRTLGLRSVAQLEWAAMNPVVRPWVEAFADGVS
                     QWFAEIREGRAALPPAGPAWQASHFEPFSAIDALLIDKLMQFAMSWSIGQELAATQFT
                     ALVRATQHSPNPKVRQRSGMLPDLLRFAPSRPEITVESYSLDHGSSLSPRDGLPANIP
                     MIPHLSALTPSLEAWKRAGLAFGAKGNVGTNAWAVAPLRSVSGGSVLAVDAHFRLTSP
                     PPLWLVHLQIANAHGVDGPYVTGASLPGVPGVAYGFHRDLAWSPVASSFDVADVYVER
                     LDATAQAVQVGNDSIPLEVRQETIAVAEGQDIVIDVRAVPHHGPIIPILVDHQVAPLQ
                     PDLGALSVRWSGLSPTRDLEFSLSLLAAEGVEHVLSQAGFAPSSVRSHVVADVDEVLY
                     VAPTLVVRRDDRALAWDARKYEGRLPCLALPGDGTAEWVGTYALSSIPWVQRPSSGYV
                     VTANADPVGTTLDNDPSNDRLADGSSAFFSCSFDIGFRQGRLRDKLQAAVEPITLADA
                     ISIQSDVGSDLAARLVPHWVDAVKRVQREQQKPGTYPLLTELAHSSRFATFDADHWLG
                     LMESWEKDAQFEMRTGVTTADMSLTLDSRDALASRASLMFHAWLTRLFHNVFADEMGA
                     MGLGMQGWLAISESLPVRTLLHLLETRAESLASYDMSLRDSLLWDDLTTDPLESRDER
                     LVTSWLDAIDWLSEHLGDDRNRWRWGLVHTVRLAAPDPSMGLLAVPSESDRIFPNGFP
                     RTGGLYTVDTADYSLARLGPGSPPLFAVHEGPALRLAVRFTSNGPEAAVALAGGQIAD
                     PARSYFRDGMELWRHNLTHALIYRRNEVLNQARTREVAVSRKDGSVD"
     gene            14935..15972
                     /locus_tag="PK710_02445"
     CDS             14935..15972
                     /locus_tag="PK710_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT08596.1"
                     /translation="MIFISLNPPNAQSWSYLIADGQEAIVVDPVQEYLERYESELRQR
                     RLRLVAVVDTHTHADYVSGAAELSRRSGAPLVMHHQAPRACVQKRVRDGDELMVGALR
                     VKVLSTPGHTYDSISLWLPDRILTGDALDQGAMGHSGESCSNAEAWQETVQRFERLEP
                     TLSVYGTHVDPSRPRVLMLALAQALQRHGDGGGSKSTSRVGNDFRQPTEGRSFDALRS
                     NLACAAVESRPAGDVGQEAVRRLSPSELVEALRSERAPQVIDVRSEDEYHDATLGRVP
                     GALLLPLEHLEAQGESLRGRPGGVVVSCRTTARAVLAASVLQRQGLTDVSVLEGGVLA
                     WKAEGHPVELG"
     gene            16215..17717
                     /locus_tag="PK710_02450"
     CDS             16215..17717
                     /locus_tag="PK710_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08597.1"
                     /translation="MHEFSLNMGIQAMSLLAVALLAGCSGDSFDDKAVDTSSGKAVDA
                     AADTSVDGGGIDGATEGSADVVQEADEAEAEACDPSEQPPEAVFVSADNGDDGTGDGS
                     ASSPLGSVDTALALAAKKKAAFVVLDEGTYKEAITLDSAHGGVTITGGWKMDGTSWKR
                     NCSAQARELAVIASPTRVGLEGKHGAEGITLRTLTVKSTESGGANPNEPGKSCYGILV
                     HDAHLTLEDVVVEACAGQSAGDVTLYFGNDPPDCSSLETNCSKGNDGDPGDNGNSADA
                     GSYTPDGYLPANGDNGTPGANGENGKKGGLAIDDDCLECPTACLNSTCNNAAVVTVES
                     QGKCGCGGEGGYAGSGGPGGGGSFGVYVEGSKAVVKILGSLVRAREGGDGAAGLPGIP
                     GTSGSAGDPGPEKKCVNKCLPIISWGNCYCTPSNSQTTMVKGFEGGKGGDGGNGGNGG
                     GGAGGPSIAILQLGAGKVTRYGTELHVSAGGKGGDSDAAVGMAQEEYKVP"
     gene            17880..19409
                     /locus_tag="PK710_02455"
     CDS             17880..19409
                     /locus_tag="PK710_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08598.1"
                     /translation="MMQGQSSKTSIGASAMALLASLYLVSCAGDPFTSDVGDASAEKP
                     VDAAVDRPEDTLGDGEVVDGAVEGSADVIQEASESGAGDCDPLEQPPQAVYVSADKGS
                     DTTGDGSASSPLGSVGKALAVALQKNLMLVVLDAGVYSESISLQNSHGGLTIIGGWKL
                     DGNTWKRMCSPNARNDTVLASPLPVGLEAKYGSDAFTLRTLTVKATLTENAQPNAPGK
                     SCYGIMVHGATLILEDVAVEACAGQSGGQATVTIDATSPNCTSLETNCSDGQSGNDRD
                     PGVSAQQGKYLPHGYEPGNGQNGLPGVTGHNGTAGETKISDPVCIDQCRHPCANNSSC
                     DTSASSKSSITVRGTCGCGGKGGSGGGGGRGGGGSFGIYAEGSNSIVKLIGSSVRALD
                     GGDGTVGAPGTSGLPGTTGNPGEASCVLNCRYETTTYGCQCVQNKTETVQANAGGPGG
                     DGKRGGNGGGGAGGPSITLLQLGGAKITWVATALSKSNGGKAGDSKAAAGMAQEQYIV
                     P"
     gene            complement(19747..20439)
                     /locus_tag="PK710_02460"
     CDS             complement(19747..20439)
                     /locus_tag="PK710_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08599.1"
                     /translation="MNRFALAMGGRLALQRLRGRSTLLIAATSGAFCTLAAWAERHAD
                     PASAASHALRGASFGLIIPLATLAVVTLVFQRHRPDDAVGCAAVLGANRRLIVVGALL
                     ATSIISAFLGLVIAALTVLVAFARIDALVVTDLLTSAWIGALSGLGYTWYFGAASTLG
                     RRGGGRWLAWVFDWLFGSTLGVGATLFPKSHLLNLLGAEPVMGLTQKQSFAALLGIAF
                     VSAAVLVARIRK"
     gene            complement(20436..21410)
                     /locus_tag="PK710_02465"
     CDS             complement(20436..21410)
                     /locus_tag="PK710_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT08600.1"
                     /translation="MNQPVLRTHALTARDEQGGIYGLELALPAGPVGALLGTPSDGSS
                     ALAKTLAGLLRPTMGSVLIAGKNPHQSPQLRRSLGVLMPDANLADVGYVRELIAFARG
                     LRGTEAPRHSWYEPLGIDSLAPRQVASLNPREARTVALALALAVASPTALILFDPLAA
                     DVTAHTLRPLLKEKAEQGACLLVITPSVQDAIALCDDMATLERGRIGRALGQPDVDVL
                     VPGMPAELRVWCDHPRRLLSALSLAAEVKSLSWTEMHEGGMVLVRCSGAESCAKTIAK
                     AALQTGVEVRAIQQTIPGTPEVYAASAGIMLAKHHQAAYAATVAEERS"
     gene            complement(21407..>21640)
                     /locus_tag="PK710_02470"
     CDS             complement(21407..>21640)
                     /locus_tag="PK710_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08601.1"
                     /translation="LSLLPLAGFATAVGLVTGPLAGTCGWLAPRHGRSCLAVVVFIPW
                     FLDGLGTTSHAGSNSILGLLGTLADLVTRVGTS"
BASE COUNT         4417 a         5588 c         6940 g         4695 t
ORIGIN      
        1 ccgccgtcgc aatccgcggc ggatttgcat ccattcgagg catcgatgag gactcccccg
       61 cctgttcctc ccgagccgcc gaccgcgcca cccgtcccgc ccgttccgtc ttccgaaccc
      121 gcatccgatg gttttcctcc ggaaccggat gatgcggaat tgtcttccga tgatccgcat
      181 gccacgggca acaagggcag accaaggaga aagcaagcga cggtgagggt tcgtggtgaa
      241 agcacggcaa ggctcctggt tggcgggcaa tacagaaagc taccgcattt ggggtgaggg
      301 ttccactgct cggctcacga cgaatccgca aacgatagga cgatatgatt ttttgggcgg
      361 gcgcggagcg gatggtcggg gctggggagt caggattcgt gagcggcgag gaatcgttct
      421 gcttcgaggg cggccataca gccagttccg gcggcggtta cggcttgtcg gaaaacgaaa
      481 tcctgaacat cgccagcggc aaatacgccg ggcacgctgg tttgggttgt tccgggcttc
      541 gttttcacat acttattttc gtgcagttca agttggcctt ggaccagtcc cgtattgggc
      601 gtgtgtccga ttgcggcgaa catggcgctc acgtccaggg tcgagacttc accggtcttg
      661 agatttttga ccttcaggcc tgtgacttcc cctttggctg ggtccaggac atcgacgacg
      721 acatggctgt aaagcacgcg tactttgggg ttatcgagca agcgtttttg catggcctgg
      781 cttgcgcgaa actcgtctct tcggtggatg agtgtgacgg atttgcataa tccggccagg
      841 taggttgctt cttcgagcgc ggtatctccc ccgccgacga cggcgacgtc tttgtttcgg
      901 tacagggcgc catcgcagac ggcgcaagcg ctaacccctc gatttttcag cttttgttcg
      961 ctatccaggc cgagataatt ggcgctggca ccggtggcga ggatgagggt tttggcgaga
     1021 tacggttgtt cttcgtcggt ccagactttg aaaggtcgtt cgctcaagtc gagtttggtg
     1081 acgtcggcgg tgatgaaggt ggttccttgt ttttcggctt gatcgcggaa gcgttgcatc
     1141 agctccgagc cttgtatttt ctctgggaag cctgggaagt tctcgacatc ggtcgtgaac
     1201 atgagttggc ctcccggaat caacccaccg gcggaaaatc cctcgataca caggggttga
     1261 aggttagccc gggcggcata gatggcggcg gtgagcccgg cagggccgga tcctacgatg
     1321 atcgtattat gaatttgcga agacatggga acgctttacc acgtttttgg cgagtgggtg
     1381 gatagcaccg ttgggttcgt tgggtttgga gacacgacgg acaaaaggga ttcgcatatc
     1441 tcgggaatct tcgaacggag agcggaaacg ggcccggcca tcatttaacg cttgcagcgc
     1501 accatcacga agcctacgga tgcagatatg gctgaaggac taaatcgagt cattttgttg
     1561 gggaacctcg gggcggaccc ggagttgaag gtcacgcaag gcggcgcttc cgtgctgaaa
     1621 ctgcgtctgg ctaccaccga aaagtatgtg gaccgcgatg gggccaagca agagcgcacc
     1681 gaatggcatg cgatcacgat ctggggcaag cggggcgagg cactgaaccg ctttcttacg
     1741 aaaggctcgc gcattttggt ggaaggatct ttgcgcacga ccagctatga agaccgcgac
     1801 ggcaacaagc gatttcgcac cgaaatcgtc gcatccaatg tggtgttcgc gggaggggga
     1861 ggagctccac gatcccgctc gtcgtcttcc gaaggggact tcgacccgcc gccagctccg
     1921 cgggcagcaa gctctcatga cagccctccg gaggctcctg actattccga tatcgacaac
     1981 gaaattccgt tttagagcat catgttccgc tttcggtcat ggtttcccat cctcgtgctt
     2041 tgtctgacgt cgcttgtgac gtttggctgc gacggatgta aaaaagccga caacaagcct
     2101 caggtgattc atgaattgcc cgaggtgcct gctccggctc atcatctggc ggatctattc
     2161 gttcccaagc cggaggaaac ctactccgct tttcgcacgc atattggcgg tccgctggcg
     2221 ttgttgccct cgaccttccc atcgatggtc gtgacttcgt tggggcttac gacacagctt
     2281 ctcgagcaga tcgatggcaa gtcgcccatg gttggcgtca tgaccgacga tggctctcgg
     2341 ctcgtcgttg tcgttgggat ccacgttcga gatggcaatc gcacgataca gctcatgacg
     2401 gaaggggcag atgcgaagta ttccgcgaag ccagccgagc gcggggtgat tgtgctcgag
     2461 ccgaagccaa cgttgacgag ccgtgtagcg acgttgggtg ttgcgggaca ttatctgatg
     2521 acggcggaga aagcgcagga tttggtcgaa tgcgggccgt acgtgagtgg cacgttgtcc
     2581 aagcgcacgg gcatcgaagg gcaaatcgtt gtggaaacgc atcaagatgc cctgaaaggc
     2641 cccttggcga agcgcatgcg tgaagcttgg gcgtcgttca aagcagcacg ggagaaggaa
     2701 gacgacaagt tgcgcaaaga gcgggggcga agtccggatt ttggtgagcc tgcggcggcg
     2761 cttgcggacg taggagacaa agtcgaaacc atcgtttcgg tattgtccga tctgaaacga
     2821 gcccagcttc ggatagactc ggatgcggtg ggaattcatg ctttgctgac catggtcccg
     2881 gcatcgatcg aaggcgtggc agccaaagag ttttcttcgt tggtggtggg ggatctcgct
     2941 ccacttctgg cgatgcctca agacacggtg gttgggttat tggttcgaga cgatgaggcg
     3001 agtcgggagc tgggagcgaa aaaacatgcg gagggaattg aaaagcttct tggagaacga
     3061 atcaaggagc cagaaaacaa caaagtaaaa gaagccatgc tgcagtggtc caaggggcga
     3121 ggcgactact tggcggcgtc gttgcgttgg actcgcgacg aacaagaggc catcgcgcgt
     3181 ggagctgtcc gcgaccgagc ggagtttgag cggggaatcc gtagtgttct tgggttattg
     3241 gccatccctg ccatccggga gccggtcgaa catcatttgg gcaagcttac gttgtcgaac
     3301 gtgcagaaag tgggccccgg atttgcgatg catgtcgacc gcaacaaaaa agtcccagaa
     3361 ggacagcccg cccagagcac atcgttcgat gtcgcctggc gggtcgacga ctcgacattc
     3421 gaagcacgag tgaaacctga tgggaaagct tggttgacga aggatgaaga gaaaaggcca
     3481 tcacttgccg atcatccgac cacgtcgaag atatttgcgg gcctgggagc tgatgcctct
     3541 tttgtattat ttttcgaccc tcaattgttt ttatcgacct tggttccacc gagtatgaaa
     3601 gcgaaggaga cgaatgcgcc atttgtgatt gcctacggtg gagccaatca acagggatgg
     3661 ttcaagctcg cgctttctca tgcatcggct cgcgagctta tcaaaatgct gggacgacaa
     3721 gagttgaagt aacatccgtt tcgagccacg ggggtgcccc aatgtcgaca gaagaagaac
     3781 tgaaagcgga actagaccga ttgcgcgcgg agaacgaagc actaaaaaac cgaaaagcac
     3841 cagtcgtttc catcaaagtc agcgccaaag gcggtgtatc cgtctacggt ttgggacgat
     3901 ttccggtgac tttatacaag gagcaatggc tgagattgct cgacatggga gaggaaattc
     3961 gcgctttcat tcaagacaac gacgcgaatc tcaaaacgaa aagctgacgg gctctgtggg
     4021 cacatttcca tcgaccgcac ggcaagtgtc cgtgaggggg tgagttgtgc gtcgtttttc
     4081 ggagcacggt ggttgctccg tcgaccgtcg ttgaaagaat cgcagttttg ctcttggcgc
     4141 ggtcgtgcaa accgagggtg aatggcaagc gaacgagccc cttgcgaatc gatgggggtt
     4201 tttgtgcgct ctggcgcgag ccgcgctagg atagggttgt ccttacacgt acaggggtga
     4261 tggtcatgcg tactccgatt cacattgcgc tcatgttggc cgtggtgtcg ggctcgttgc
     4321 tcaatgcttg cggcgctcgt tcgcctatgc aggaacaatg ggcagacgct ggagtggaac
     4381 cggatgcaac cctcgatgcg ccttccgatg tcgtgtacga tcacgtcaat gatatcaatg
     4441 gttgcaaccc atcgacctgt agcggttgtt gtgatttgca aggcaattgc cgtacgggta
     4501 cccaaccgtc cgcttgtggt cagggcggat atgagtgtgt ggattgttct tccctaggcc
     4561 aaacctgcat tacatcaacc cgcacttgcg gagccgtaca accctgcaat ccctcgactt
     4621 gtccgcaggg gtgttgccac aacggtcttt gcgtggaggg ggcatctcac gaagcttgtg
     4681 gaagccacgg aatggattgc gtgaattgcg cctccctggg tcagacctgc aacccgtatt
     4741 cgttgacctg cgaacagagc agcatgtgtg atcccaccaa ctgtgcaggc tgttgcgacg
     4801 cctggaacca atgcaactgg gggggagatc cgtgggcttg cggttttgga ggaggggggt
     4861 gcgaggactg tgcgtcccaa ggattgacgt gcgacccgtt ctggggtggt tgtgtcacgg
     4921 tggagccttg cgggccttgg aattgccttg gctgctgtga catggtgggc aattgtcaat
     4981 ggggggccga cgactgggcc tgtgggggca acggagagct ttgccaggat tgttcagcat
     5041 tcaatagtag ttgtgactct caaacccata cttgcacggg aacgcaattg tgtggaccgg
     5101 agaattgcaa aggctgctgc gacccctatt cgggccagtg ccgcatgggc tgggacccat
     5161 gggcatgcgg ctcggcgggc caaatctgtc aagactgcac gggttccaac atgatttgtg
     5221 gagactcgcc ttttgggggc ggccaatgcc tgagcatcag tcaaacttgc ggccccaatt
     5281 gcaagggctg ttgtgcttgt aacaccacgg gctgccaatg tctcggcgga acagacatca
     5341 aggcctgtgg gcacgatggt caaatctgtc aggactgcac gattggacag gggcaatgta
     5401 ttcatgagcc aatgggcaat acgtgtatca gcacgccacc accacctact tgtggtccgt
     5461 ggaattgtcc gggttgttgt gtagacggca cccctccgac atgcgtcgaa gggcttgacg
     5521 cctggcaatg cggatggggc gggcagtttt gcagcgtatg ttatccgggg gaggcttgtg
     5581 tttggcaacc gtggggggga gggcaatgca tggccatgac ggacgggggg ccgccggatg
     5641 gggggccggc ggacgctggt atggcggacg gctttgcggg gtgcaatgct tccaattgtg
     5701 cgggatgttg cggagccaat gggcagtgtc gagcaggaaa atccgtccat caatgcggcc
     5761 gaaacgggga gctttgtgaa aactgtgcgg tgaaaaatca ggtatgctcg gacggcgact
     5821 gtaaaaaccc ttgaccccat tgcctcacga atctttgggt acgatggttt cctccttggc
     5881 aagtgcggta ttgctcactg gttatccgag ttttttggcg cgacggatgg ccgagcagat
     5941 tcttgcccac gagccgaaca cgcaattgta ttgcgttgtg aggagcaaat tcttgccctg
     6001 ggcgcagcaa acgcgtctga cgttgccggc ttctcagcgc aaccgaatgg tgttgctcga
     6061 gggggatgcg gcctcgatgg atatgggatt gtctggggca gaatatcgag aagttattga
     6121 aaaggttgat aaaattcttc atttcgccca gtaccgggat gtgcgagcgg accctcaatg
     6181 ggctcatcat acgaatgtac agggagcgct ggaagctctg gagatggcct atgcggcccc
     6241 tcgtttgacc catctcgtat gcatgtcatc catcgacgta ttggcttgtt ccgggctggc
     6301 tcgtgaaagc gatcctccaa aatcaccgcc attccggact ttcatccagg aatccttggc
     6361 ccatgcggag cggatttatc gccgggcggc gcatcgaatc cccgtgacca tccttcgccc
     6421 ttccaccatg attggcgaca gcaataccgg agaaatcgat cgtctcgatg gcccctatcc
     6481 gctgttcatg ttgctggtat cctcgggcag ggacacgatt cccctacccc ttcgtgctga
     6541 tcatctgctg cacttcgtgc cggtggattt cgtgattcga agtgcgcata tcattgccaa
     6601 ttcgccatgg tcgccgggca agacctttca cttggtcgac cccgccccat tatcggctcg
     6661 gcgtgtattc gaattggtgg cgcgggtggc tgagcgtcgc tcttcccgtg gcgatgtgcc
     6721 gagtttcgtg gcccgtgcgt tggtgcgaag tcctggggtg gaaagttttg ctcgaagtcc
     6781 ccgtgcattt cttgatgact tttctccacg ggctcgtttc gatgctacca acacggaagc
     6841 tatcttgcag ggaacggggg tgagttgtcc tcctttcgag tcttacgtgg aagccattgt
     6901 gcgctatgtg caagggaggc ttcatgagca acgggagggc ggcgttcatc ccatgcacga
     6961 tccacttctt tgaaaccaat gctcaagcac ggtctatttc gttccaaagc gcatcggcag
     7021 gttgcggtat cggcgtttgc ggtagtgagt tccgccgcgg tggtagtgac atgcagtgcg
     7081 gtggggccca gacccccaaa agtacaagaa gtacaagaaa aggaacagaa ggagccgaca
     7141 acgccaagct tggtccaagc cgcagtatcg agcgctgcga gccaggcgct ggctgaacca
     7201 tcccccccgc aggttggacg atggtttgcc ccgtcgggtc tcgggatgac ccccgaaggc
     7261 aatgccgtgc ctcttttacc caggttttac gatgccttgg cgcgactcga gacgcgtctt
     7321 cggcacgacc atgtgcgcat cgtatggctt ggggattcgc atacacaagc ggatggttgg
     7381 acccaggcgg tgcgtcgcgc gttgcagcag cgttttggta acggaggtcc ggggttcatc
     7441 catctggggt ggaccacgta tggttatcgt cgagaaaacg tgatccttcg agcatcgggg
     7501 gcgtggtcgc tgcttcccac cacgttggta gcggtcacgc gggttgacga tggggtattg
     7561 gggcttgggg gattgcggtt ggagcctcgg ggtttggatg ccacggcgca agtgggcgtg
     7621 aatgcggccg atcttcctgg agaagggcta tgggatttgg ccgttcgatt gatggaaaaa
     7681 gactcgagtt tggtgattcg tctcgagggt caggagcatc gtgtcgaagc gcaatctttc
     7741 acgcttgggc gcattcggca tgtgacactg cgttcgaaag ggccgggagg aacgctgggg
     7801 gtcgagcagg gttggaatcg accgcagctt ctgggcttgg tggtggaagc cacggacagg
     7861 catggcgtgg tactcgatac gcttgggttg aatggtgcac gctatcgttc ggcgctagcg
     7921 tgggatgaag cggcctgggt gggagagttg gcgcgtcgtg agccagattt ggtggtgctt
     7981 gcctatggga cgaatgaatc gagtgatatc aagctgaaag cggagttgca tgcggcccgg
     8041 gtcaaggaat tggtggggcg cgttcgcaaa gcagcgcctc acgcggattg cttgattttt
     8101 ggtcccatcg accggggagg ggagcaatac gaggcgacgg tcgcgcggat caacgaagcg
     8161 caagcggcgg cggcgcgtga gttgggctgt gctttttgga atgggcaaca ggccatgggc
     8221 ggagcgggaa gcatgcagcg atgggcgacg atgaatccac cgcttggagg gggcgatcgc
     8281 gttcatctct atccccgtgg ttatcagaag ttgggtgaga tgctcgcgcg agacttgctc
     8341 actggttacg atgccggggt ggtgcggggg caaagcccat ccgacgatcc cgaacgatga
     8401 cctctttcgc gccatcctcg agggaaaagc aatgattcga tagctgggtt gcttgtcgcg
     8461 ctatcatcag gctgagccaa tcgtggggtc gtatggtcat ttctggcttt ggtgagtctt
     8521 tgttctcctc gcttttcgtg ctagcgctat ccgtcaaggg tcctctcgtc attgaataaa
     8581 tactccgtgt tgtattcgag ggatgccctt ttccaagaat caggaggatg catgcgtcaa
     8641 ttcaaaggtt ttcttcaagc ttcgttcctt ggtttggcag gcttgttggt atcgacggga
     8701 gccatggccc aaggcccagc agcccccgct cccacggagc ctgatcatag ctcggtggtg
     8761 ggttcgttgg gtgttggcta tctcggcgcc tctcaggtgg cgattggcgt gggcggaacg
     8821 acgatgactg ctcccgtggt gggcgtacgg tattggctcg acccgatgct cggcttggat
     8881 ttgggactcg gcttgggcat gacgagcggg aaaacgaaaa catcaccagc aaatggtcct
     8941 tcggaaacgg aggacgatcc cagttttacg gcgttcatta tccatgcggg tgtgcctttg
     9001 agccttcgcg aaggaaaaca ctatagtttt caaatcattc cggaggcgaa cttcggaatg
     9061 ggtacgggaa gcgcggaaag gggcgaagct gacatcgact acagcggcat gaggttcgat
     9121 attggcgcac gagcaggtgc ggagcttcat ttcggtttca ttggagttcc cgagttgtcc
     9181 ttacaaggca gcgtcggcct tcaattcagc accgcgtcgg ccaaaacgga aaccaagcag
     9241 gcggaccaaa aaagcaaagt agaagcctca gctacacggc tgggtacgac cgttcacgac
     9301 aacccgtgga atattttcac aacgaacgtg gcggcacttt attatttctg atctaaaata
     9361 gtgaacgtac cgcgtagtca ggaggtttgc catgacccag ccgattcgca atatcgttgc
     9421 cccatgggcg ttcctcatgc tggtcgcttg tggcgacggt ggttcgggcc aatctggact
     9481 gagcctgaac gacgggaacg gcgagccaac gagttcttgg acagaaaacc cgagtagtaa
     9541 tacagaaacc ccttctcgtt ccggcgattc attaccttcc aaccatgagt ccagcggatc
     9601 gtcgggggaa acgtcaggtg gctcgaatgg ctcgtcggag gggtccgcaa ccgctttatg
     9661 caaagccgtt tgcaacaaat tgtccgactt ggcttgtaca ccagtgtccg aaagcgagtg
     9721 cctttccgct tgtgcagagg agtacccaga gcttatctcc cagatgggag gggagcgctg
     9781 cttgtcagag atcacggccg tgttcaggga ttgtacgttt tactgtgata tcgaaggaga
     9841 gttcgcgctc gatccggact cctgtccaaa ccagttgacg gcactgtatg aatgcgccgg
     9901 aggggatttt gatttcgatg atgacgtcgg taacgacacc accgaaccct cgtgcaagcc
     9961 gccatcttgt atgggctgtg aggatgtgtg ctcgacttgc ttatgcagca caggggactc
    10021 ggagatctgc gcggaatact gcgagtagcg cggcccttcg cttctataat ttcattatac
    10081 atttcaattg gttacgattt acgcgcgcca gaggactcat cgtcttgcgc atcgtggatc
    10141 ggttcaatgt acgctgccga ccacccaggc ttgccctgcc acgagcactt cttcgggtcg
    10201 tgcgcccgcg gccatggccg gaccggttcc tttttcatgg gagggcagca cgaaaccgtg
    10261 atcgccgaag atgaacagca gggtttgggc aggtagttgg gaagtaagct ggagcaaagg
    10321 gaatgcgacc gcgcgggcga gggcatcgag tcgctgggct tctggcaggc cggaggtggt
    10381 gaggtcggct tggatgacat cgagtttatg cagttctcgc cctcccactc ggatacgacg
    10441 aaggatcgag gcggtacgtc cgcgtccgat aggcaattcg gcttctcgca gttcttgtga
    10501 gtcaaggggc tcggataagg cttgaacgcc tcgctccaac aatcgaagtt gcacggcggt
    10561 caccgcgggc aatgcggacc aaagcaatac ccgttcggcc agcaccgccg tgccgcgcaa
    10621 ctcttcctga agcacgtgtt ccaatcgcaa tcccacgtcg tagcgcatgc cgtcgacgag
    10681 aaccaacgtg atcccgcggg tgccgtggag tcttccgatc cgcgaggcga tgccggggat
    10741 atcgagcacc atcgtcggcc gtttgtgggt gacttttgcg gcgatgaaag cctctttata
    10801 gcttttctcg aacgcttccg cgaactgcag caggctctgt cgcgcgcgtc ggtcgttttc
    10861 tccttgtcgg acggcttcgg cgagaggggt ataggcttgc tcgaagagtt tttccaccgc
    10921 ggacaagggt ttgggaccgt gggcatcgtc gatgagtttg gcgtagtgtc gccaatcttt
    10981 gggggatgtt acggtcgatg tcagcgcggg ggcagtggag ggcaggatgg ttcttgtgtc
    11041 taattccgcc tttgcgccga gcaaatcttg cagcgccaga gggggcccat aagctcgaag
    11101 gctcacatcg gtggaagaca gccatagggc gaaggggtag cggcttgccg cgctggaata
    11161 aaaacgcaag cacgcgctat cgtcgtcgcc gagggcgcct tgggcgccaa tgagaccgga
    11221 tagggattcg agggcgacgg caatgccatg ggcgccgact cgttgggctc gatacaattg
    11281 gtcttcgatg atttgcgtgc tttcggcgtg aacgttcaag ccggagggag gagcgcctcg
    11341 tcgttgtagc gctgtttcca cggcttcttc gataagtgtg gtgagttgtc ctcgggctcc
    11401 tggccccaag ggaggcacat tcaccatcaa ccatccgctt gattctgccg cacttcgcaa
    11461 ccgcaccctg gaggaagccc ccagaaacct cacctcgcca atttgctgcc agcggggatc
    11521 ggatacgttt gccaagctca tggtctcaag cttgccgaaa acctttcgga tggcccagtt
    11581 ttgcgtctgc cgtccttggt atcgtgccca atatcacgaa aaacccatat tttacctata
    11641 tttactatta ccctactccc tcgaaacccc gcgggcctct ttgttggttt cacgggttcc
    11701 acggttttca ttttcccgct accgtctgca catgtccatt cggcatcgag caccgtgggc
    11761 aatcgcgtct ctcatcgctt tggtcggctg ctgggattca gacggatacg tgcggccccg
    11821 gccttcgcct acttccgcgc cgccggtcga aaaagggccc ctaggcaacg agttgaacgt
    11881 caatggcacc atcgaccttg cgggtctttc tgcgccggtc gacatcgtgc gcgatacgca
    11941 cggcgtggtt catatctatg cccagaccca tgcggacgcc tttcgagcgc agggatacgc
    12001 gatggcgagg gatcgggcgg cgcaaatcga gttgttgcga aggacggcgg atgggcgaac
    12061 ggcgcaaatc cttggggccg tatcgccttc gaccatcgat caggacatcg tgatgagaac
    12121 gcttgggctt cgttccgtcg cgcagctcga atgggcggcc atgaaccccg tggttcgtcc
    12181 gtgggtcgag gcttttgccg atggtgtttc tcaatggttt gcggagatcc gcgaaggtcg
    12241 agctgctctt cctcctgctg ggcccgcatg gcaagcttcg cactttgagc ctttttccgc
    12301 catcgacgcg cttctcatcg acaagttgat gcaatttgcc atgtcctggt ccatcgggca
    12361 ggagttggcg gcaacgcaat tcacagcgct ggtacgcgca acacaacaca gccccaaccc
    12421 aaaggtacgt cagcgttctg gcatgcttcc ggacctgcta cgttttgcgc catctcgccc
    12481 tgagatcacg gtggaatcct attctctcga ccatgggtcg agcttgtctc ctcgcgatgg
    12541 gctgccagcc aatatcccaa tgattccgca tctatccgcg ttgactccga gcctcgaggc
    12601 gtggaagcgc gctggccttg cgtttggtgc caagggcaat gtgggaacca acgcatgggc
    12661 ggtggctccc cttcgttctg tttccggcgg ctcggtgctc gcggtcgatg cgcattttcg
    12721 tttgacctca cctccgcctt tgtggctcgt tcacttgcaa attgccaacg ctcatggcgt
    12781 cgacgggccc tatgtcacgg gagcttctct gccgggtgtg cctggtgtcg cttacggttt
    12841 tcaccgagat ttagcctgga gtccggtggc gagttccttc gatgtggcgg atgtatatgt
    12901 cgagcgtctc gatgcgacgg cgcaagcggt gcaggtggga aacgattcga tcccccttga
    12961 agtgcggcaa gagacgattg ccgtggccga agggcaagac attgtcatcg acgttcgcgc
    13021 tgtgccgcat catggcccta tcattccgat tcttgtcgac caccaagtgg ctccgcttca
    13081 gcctgatttg ggggctttat cggttcgatg gtctgggctg agcccaacgc gtgacctgga
    13141 gttctcccta tcgctcctgg cggccgaagg ggtggagcat gtgctatcgc aggcgggttt
    13201 tgctccttct tccgtaagaa gccatgtggt ggcggatgtg gatgaggtgc tttacgtggc
    13261 gcccacgctc gtggtccgtc gcgacgatcg tgccctcgcg tgggatgcgc gaaagtacga
    13321 gggtcgtctt ccctgtttgg ctcttcctgg tgatggcact gcggagtggg tgggcacgta
    13381 tgcgctttca tccatcccat gggtccagcg tccgagttct ggttatgtag tgacggcgaa
    13441 cgccgaccca gtgggtacga ctttggacaa cgatccttcc aacgacaggc tggcggatgg
    13501 gtcgtcggcc tttttctcct gcagtttcga catcggtttc cggcaagggc gtcttcggga
    13561 caagctacag gccgcggtcg agcccatcac gttggccgat gcgatatcga ttcaatcgga
    13621 cgtgggttcc gatttggcgg ctcgattggt gccgcattgg gtcgatgccg tgaagcgtgt
    13681 gcagcgggaa caacagaagc cgggcaccta cccgttgttg acggagctgg cccattcgag
    13741 ccgtttcgcg acttttgacg ccgaccattg gctagggctt atggaatcat gggaaaaaga
    13801 tgcacaattt gagatgcgta cgggtgtgac gacagccgat atgtccttga cgctagactc
    13861 gagggatgcg ttggcgtcgc gtgcttcgct gatgtttcac gcctggctca ctcgattgtt
    13921 ccacaatgta tttgccgatg agatgggggc gatgggccta gggatgcaag gttggctggc
    13981 gatatcggaa agcttgccgg tccgaacttt gctccatttg ttggaaaccc gagcagaatc
    14041 gcttgcaagc tatgatatgt ccttgcgtga cagtctgttg tgggatgacc tgacgacgga
    14101 tcccctcgaa tcgcgcgatg agcgtctcgt cacatcgtgg ctcgacgcca tcgattggct
    14161 cagcgagcat cttggcgacg ataggaatcg gtggcggtgg gggttggtcc ataccgttcg
    14221 tttggcggcg ccggatccat cgatgggtct tttggcggtc ccgtcggaat ccgatcgtat
    14281 ctttcccaat ggttttccga gaacgggagg attgtatacg gtagacacgg cggactattc
    14341 gttggcgcgg ctgggtcccg ggtcgccgcc tcttttcgct gtccacgaag ggccggcgtt
    14401 acgtcttgcg gttcggttta caagcaacgg cccggaggcg gcggtagcgc ttgcgggagg
    14461 ccaaattgcg gatccggccc gtagctattt ccgtgatggc atggaattat ggcgtcacaa
    14521 ccttacccac gcgctgattt accgtcgaaa tgaagttctg aaccaggcgc gtacgcggga
    14581 ggtggcggtc tccaggaaag atggttctgt ggattgacca acgcgcgggc tgggtttacg
    14641 tttgacaggc tccctctgtg gggcgcgcca ggtggaagca agaagaatcc cgattgcgat
    14701 tgagtacggg ataccgaatg agtcgtggag agccaggccc ggaacctcct caccgggagg
    14761 ccagaagcga caataggtat cccacctagc gatagctagg ggttcagcat atctgccagc
    14821 ggccttcacg gtacggagcc tattttgtcc gcggcccaac tatcccactt cggatagttc
    14881 cccttggcgg aaccttcctg tcgtgtgggt gctcctacga cgacaggtgc cacaatgatt
    14941 ttcatttctc tcaatcctcc gaatgcacaa tcctggtcgt accttatcgc cgatggtcaa
    15001 gaggccatcg ttgtcgatcc cgtgcaggag tatctcgagc gttacgaatc ggagttgcga
    15061 cagcgacgcc tgcggctggt ggcggtcgtg gatacgcata ctcatgccga ctacgtttct
    15121 ggcgccgcgg agttgtctcg tcgcagcggc gcgcctttgg tgatgcatca tcaagcgccg
    15181 agggcatgcg tacaaaaaag ggtgcgggat ggtgatgaac tcatggtggg cgcgcttcgt
    15241 gtgaaagtgc tttcgacccc cggtcacacc tacgacagca tcagtttatg gttacccgat
    15301 cgaattttga cgggagatgc gctcgatcaa ggcgcgatgg gccattcggg cgaaagttgt
    15361 tccaatgcgg aagcgtggca ggagaccgtg caacggttcg aacgattgga gccgactcta
    15421 tccgtatatg ggacccatgt cgatccgtcg aggcctcggg tgttgatgct tgctcttgcg
    15481 caggctttac agcgtcacgg cgatggcggg gggtcgaaat ctacgtctcg tgtaggaaat
    15541 gattttcgtc aacccacgga aggtcgttct ttcgatgcgc ttcgaagcaa cctggcctgt
    15601 gcggcggtgg aatcgaggcc agcgggcgac gtgggacaag aggcggttcg tcgtctgtcg
    15661 ccttcggagt tggtggaggc attgcggtcg gaaagggcgc ctcaggtcat cgacgtacgg
    15721 tccgaggatg agtatcatga cgcgacgctc gggcgcgttc ctggggcgtt gttgttgccg
    15781 ttggagcatc ttgaagcgca aggggagtcg ttacgagggc ggccgggggg agtcgtggta
    15841 agttgtcgga cgacggcgcg ggcggtactg gcggcttcgg tgttacagcg gcaggggttg
    15901 acggacgttt cggtgctcga agggggcgtt ttggcgtgga aagcggaagg gcatccggtg
    15961 gagcttggct gagcggggcg aatggcgcag cgacgattcg ccggacgaaa aagcaaggat
    16021 catcgaagca gcatcccctg cggaactcat aatttattca aataaatcaa tgtcttatcg
    16081 ttcaacggcc aacgtgaatc catggcgctg agcccatgtt tcccctagcc gggattggaa
    16141 aggagaacct caaaaacgtg gtagcgctta tttttatgct atcgttttgg atatcaacgt
    16201 tggagataat catcatgcac gagttttctt taaatatggg aatacaagcc atgtcgttgc
    16261 tggccgtggc gttgctggct ggttgttccg gggattcttt cgacgacaag gcggtcgata
    16321 cctcctccgg aaaagccgtc gacgccgcag cggatacatc ggtggacgga gggggtatcg
    16381 atggtgcgac cgaaggttcg gccgatgtcg tccaagaggc cgacgaggca gaggccgaag
    16441 cctgtgatcc gtcggaacaa ccgcctgagg cagtatttgt cagcgcggac aacggcgatg
    16501 atgggactgg cgatgggtcg gcctcttcac cgcttggcag tgtggacacg gcgttagcgc
    16561 tcgctgcgaa aaagaaagcg gcgttcgtgg ttctcgatga gggaacctac aaggaagcca
    16621 tcaccttgga cagcgcgcat ggtggggtaa cgatcaccgg cggatggaaa atggatggga
    16681 cctcgtggaa aaggaattgc tcagcgcaag cccgggagct tgccgtgata gcctcgccca
    16741 cgcgcgtggg attggaaggc aagcatgggg ccgagggcat cacacttcgc acgttgacgg
    16801 tgaaatccac ggaaagcggg ggggcgaacc ccaacgagcc ggggaaatct tgctatggaa
    16861 tacttgtgca tgacgcgcat ctgaccctcg aggatgtggt ggttgaggcg tgtgcggggc
    16921 aatcggcggg agacgtcacg ctctattttg gtaatgaccc tccagactgc tcttcgttgg
    16981 agaccaattg cagcaaggga aacgacggag atcctggaga taatgggaat agcgccgacg
    17041 ccggaagcta tacaccggat ggttacctac cagcgaatgg ggacaacggc acaccgggag
    17101 ccaatgggga aaatgggaaa aaaggcgggc ttgccatcga tgacgactgc cttgaatgcc
    17161 ccaccgcctg tttaaatagc acatgcaaca acgccgcggt agtcaccgtc gagtcgcaag
    17221 gcaagtgtgg ttgtgggggc gaagggggat acgctggctc gggaggtcct gggggaggcg
    17281 gttctttcgg ggtttatgtg gaaggaagca aagccgtcgt caagattttg ggttcgctcg
    17341 tccgtgcgcg cgaaggcggg gatggtgcag ccggccttcc agggattcct ggcaccagtg
    17401 gttccgctgg agatcctggg ccagaaaaaa agtgcgtcaa taagtgcctt ccaatcatca
    17461 gctggggcaa ctgctactgc acgccaagca actctcaaac aacgatggtg aaaggatttg
    17521 aaggcgggaa aggcggtgac ggcggaaatg gtggaaatgg cggcggaggc gcgggaggac
    17581 cgtcgattgc gatactccaa ctgggtgccg gcaaagtaac gcggtatggc accgaattgc
    17641 acgtttccgc gggaggaaaa ggcggtgaca gcgatgcggc agtggggatg gcccaagagg
    17701 aatacaaggt cccataaatc cgcagagcgt cgacattcgc tcacgacata gcaattcgct
    17761 tttcaatttt ctcatttgtc tttatatttc cagaattaat tcaaactgca atggactgtc
    17821 aaacagtgat agtcgtcata gttatgctat tacttcttac atcattcgcg gagaaactca
    17881 tgatgcaggg gcagtcgtcc aaaacctcga ttggcgcttc ggccatggcc ttgttagcgt
    17941 cattgtatct ggtaagttgc gcgggagacc cgttcacgag tgatgtaggc gacgcctccg
    18001 ccgaaaagcc ggtagatgcg gctgtggata ggccggaaga cacgttgggg gatggggagg
    18061 ttgtcgatgg ggcggtcgag ggttctgcgg atgtgatcca ggaagccagt gaaagcgggg
    18121 ctggggactg tgatcccttg gaacaaccgc cacaggccgt ttacgtcagt gcggataaag
    18181 ggagcgacac gacgggagac gggtccgctt catcgccgct tggcagtgta ggcaaggcat
    18241 tggcggttgc gctgcaaaaa aatctcatgc tcgtggtgct cgacgcaggg gtttattcgg
    18301 aatccatctc gttgcaaaac tcacatggtg ggctgacgat cataggcggg tggaagctgg
    18361 atgggaatac ctggaaacgg atgtgttccc cgaacgcccg gaacgatact gtgttggcat
    18421 cgcctttgcc ggttggattg gaagcgaaat atgggtcaga cgcgttcacg cttcgcacat
    18481 tgacggtgaa ggccaccctg acagagaacg cgcagcccaa cgcgcccggt aagtcgtgtt
    18541 atggaattat ggtgcatggg gcgactctga ttctcgaaga tgtggcggtg gaggcgtgcg
    18601 cggggcaatc gggggggcag gcgacggtca cgatcgatgc aacgtcaccc aattgcacat
    18661 cgctggaaac gaactgcagt gatgggcaaa gcggaaatga ccgcgatccg ggtgttagtg
    18721 cccaacaagg caagtatttg ccccatggtt acgagccagg aaatggacag aacggcctcc
    18781 ccggagtcac tggacataac ggtactgccg gtgaaactaa gatatcggat ccggtgtgta
    18841 tcgaccaatg tcggcatcct tgtgcaaaca atagcagctg tgatacctcg gcaagctcga
    18901 agtcctcgat caccgtgcgg gggacgtgcg gttgtggcgg aaaagggggt agtggtggcg
    18961 ggggtggacg tggaggaggc ggttctttcg ggatctacgc ggaaggaagt aacagtatcg
    19021 tgaaattgat cggttcgagc gtccgcgcgc ttgacggagg agatggcaca gtcggagcac
    19081 caggtacatc cggattaccg ggaactacag gaaaccctgg ggaagcctcc tgcgtcctga
    19141 actgcaggta cgagacgact acgtatggct gccagtgcgt acaaaacaag acggagaccg
    19201 tgcaagccaa cgcgggaggt cccggtggtg acggcaaaag agggggaaat ggtggcggcg
    19261 gcgcaggtgg accgtcgatt accctgctcc aactcggcgg cgctaaaata acgtgggtgg
    19321 caaccgcctt gtcaaagtcc aacgggggaa aagccggaga cagcaaggcc gcagcgggga
    19381 tggctcaaga acaatacatc gtcccgtagg tgcccgaccg ataatttttc ccttatattt
    19441 cacatgatta ccattaatga tttgaatttg aacgatcgcc tgtcccaaag gttacggcat
    19501 cgtccacaga gataggggtg atattcaact tattgagtga tacccccggc aatcatgctg
    19561 ttttcggttc gacgctcacg gaaaaaccat gatgcgcggc ccttcccggt ggtgccatga
    19621 tctcaaagcc acatacggca ttgaacgtct ccaaaggcga aaacggcagc gatggcagcg
    19681 cggcgcaggg gattgcgcag acggtgtgga cggcatcgtg attcgttggg tgaagggccg
    19741 gatatatcat tttcggatac gcgccaccaa cacggcggcc gacacgaatg cgattcccaa
    19801 aagcgcggcg aagctttgct tttgtgtcag acccatcacg ggttccgccc ccaacaagtt
    19861 gagcagatga gacttgggaa acaaggtcgc gccaactcct agcgtcgaac caaagagcca
    19921 atcgaagacc caggcaagcc atcgtccgcc tccacgacgt ccgagggtcg atgcagcgcc
    19981 gaaataccat gtatatccca atccggacag cgctccgatc cacgcggaag tcaaaagatc
    20041 ggtaaccacg agtgcgtcga ttcgagcgaa tgcaacaagg acggtaagcg cagcgataac
    20101 gaggccgagg aaagccgata tgatgctcgt ggcgagcagc gcccccacca cgatgaggcg
    20161 tcggttggcg cccaacacgg cagcgcatcc tacggcatcg tcgggtctat gacgctggaa
    20221 aaccagggtc acgacagcga gggtggccag gggaatgatc aaaccgaacg acgctccccg
    20281 caaggcatgg cttgcggcgc tggcggggtc cgcatgtcgt tctgcccaag ccgccaaggt
    20341 acaaaacgca ccggaagttg ccgcgataag caaggtggag cggccgcgga gtcgttggag
    20401 agccagtcgc ccacccatcg ccaaggcgaa tcggttcacg agcgctcctc cgcgacggta
    20461 gcggcataag cagcttgatg gtgtttggct agcatgatgc cagcggaggc ggcgtatact
    20521 tccggcgttc ccgggatggt ctgttgaatg gcgcgcactt cgacccccgt ctgaagggcg
    20581 gccttggcga tggtttttgc acaagactcc gcgccagagc agcgaacgag caccatcccc
    20641 ccttcgtgca tttcggtcca ggacagggat ttcacttcgg cagcgagcga aagggccgag
    20701 agcaatcgtc ggggatggtc gcaccacacg cgcaattcgg cgggcattcc tggcaccagc
    20761 acgtctacat ctggttggcc caaagcgcgt ccaatccggc ctcgttcgag ggtagccata
    20821 tcgtcgcaaa gggcgatcgc atcttgtacg gacggcgtga tcacgagcag gcaagcgcct
    20881 tgttccgctt tctctttcaa caagggccgt agggtatggg cggtcacgtc cgccgcgagc
    20941 gggtcgaata ggatgagggc ggtgggggaa gcgacggcga gggccaaggc aagggcgacg
    21001 gtacgtgctt ctcgtgggtt gagggaagcc acctgtcgcg gggccaaaga atcgattccg
    21061 aggggttcat accaagagtg tcttggggcc tccgtgccgc gcagaccgcg agcaaacgca
    21121 attaattcac gtacatagcc aacatccgcc aagttggcat cgggcatgag cacgccgagg
    21181 gagcgtctca attgcggaga ctggtgggga ttttttcccg cgatgagcac ggagcccatg
    21241 gtggggcgaa gcagtccagc gagagtcttg gcgagggcgc tcgagccatc ggagggagtg
    21301 ccgagcaagg cgcccacggg tcccgcgggc aaagcgagtt cgaggccgta gatgcctcct
    21361 tgttcatcgc gggcggtgag cgcatgggtt cgaaggacgg gttgattcat gaggttccca
    21421 ccctggttac caagtccgcc aaggttccga gcaacccgag gatcgagttc gagcccgcgt
    21481 gcgatgtcgt tcccaagcca tcgagaaacc aagggataaa caccaccacc gccaggcagg
    21541 agcgaccatg gcgtggcgcg agccaaccgc aggttcccgc cagggggccg gtgacgagtc
    21601 cgaccgcggt ggcgaatccg gcgaggggaa gcagtgacaa
//
LOCUS       DAOSOR010000025        21525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_2444_length_21525_cov_4.011272, whole genome shotgun sequence.
ACCESSION   DAOSOR010000025 DAOSOR010000000
VERSION     DAOSOR010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 21525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21525
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2444_length_21525_cov_4.011272"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="PK710_02475"
     CDS             <1..813
                     /locus_tag="PK710_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08602.1"
                     /translation="DDKMVLVRLAANGLAQFENIIVGGNSHQAEGDRWIDNWPHEGRL
                     AWSGSEYAAYFGHTGNWGSQGNHQGDRLEIIDATGNGGGGGWGWGCSHSLDVRIAHNI
                     QRFGPVCLSDCYPGKGIYFDHHTLVSDEPSGNCIGGSNARLGGLAAVDGGFWLTYSSS
                     EGRASSDIALVHIDDAGAVSPPQWLTNTDSEQEKFAYLARYGSGLLAAFESGGQRQFV
                     VLDASATVTEGPVPLDVPLPTGADMVTFPNGDVGWGSTDGYGLTVVRVKRCQ"
     gene            complement(878..2431)
                     /gene="murJ"
                     /locus_tag="PK710_02480"
     CDS             complement(878..2431)
                     /gene="murJ"
                     /locus_tag="PK710_02480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOT08603.1"
                     /translation="MPPSVTRRIGIASLIWGASIFLSRIIGLVREAVLGRILGGGKEA
                     DVFLASFIVPDFLNYLLAGGALSIVFIPIFSGYLAKGEEKRGWETFSIIANFLLALLG
                     IATVALWIAVPWLVSLVAPGFDEYQRSELVNLTRIILPAQVFHLVGGLLSAALQARDK
                     HSLPALAPLLYTSAIVVGGLVGGTEAGAYGFAWGVLVGSILGPFALPLIGCLHVGLSW
                     RPIFQIKHPDLRTYLFRSLPIMLGWSIVVVDDWLLGRFGSIVGASTIATLHYSKTLLK
                     VPMGVFGLAAGVAAFPTLSRLVAENSHAEAYTTLSTAVRRMLVLALAAQVVLTTAGQE
                     IATVIYGRKIPHDQYVTMGWVLGIMSVGLWAWAAQTVLSRGYYALGKTWEPTLLGTSI
                     VVLAYPMYWAMAGYGAIGLAASSAIAISVYTVVLAIRLRAYFPNVADHYGRFLLRFCP
                     AVFAGIACGLAARTLLTDTHLLIRGSIAATAGVAVFGIVAYALRAEELRDVVAWGMQK
                     ARRRSVKQI"
     gene            complement(2494..3123)
                     /locus_tag="PK710_02485"
     CDS             complement(2494..3123)
                     /locus_tag="PK710_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08604.1"
                     /translation="MRRSAWKFYLFTFFSAMPAWAQTYAAPPYPVREPEPRYSQVADP
                     PDDPYEMQSWRKSELSTFRFHIGGAGRIREDEVTPGLWTALDVGRGPAGFRFSGSWLR
                     VGSNHGVAHYTGELTLDLGGRHSWRPVLGAGAGLSRVYEKQSEETGVVAQSLGIGLVR
                     AGIEYRIPVESTDTRAGISAIGMLPAIRSDNAPDLKPWLVLAATVGVGF"
     gene            3230..3997
                     /locus_tag="PK710_02490"
     CDS             3230..3997
                     /locus_tag="PK710_02490"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT08605.1"
                     /translation="MATSLATSLVLAQPSKLSRTAIGQTSQASAEQHFGKAREYYANG
                     RYREAIEQLEIALRIDPKGAELLYNLGVIHEKLQELDPAIDYYKRYVLMVGDPEEQQR
                     VKTIIARLEKARDELGNDKEQPEFAEEKPIPVKEPERTKGRFDGWVIGTGILAAVGVA
                     GGTYFGLEALKSRNEDPPTTGKGTSYADLEDNADRAKQMALFADIGFGVGVVAGTAAM
                     LLYFMRDGEPEQAAVGAWEPGVAVVPGGMAASMKLGF"
     gene            4074..5144
                     /locus_tag="PK710_02495"
     CDS             4074..5144
                     /locus_tag="PK710_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08606.1"
                     /translation="MNRHWLLGLGVMIGVGACQLVVGETGLGTVRCQDEGAMGPPACA
                     KGQTCVKGTCVVAVNIGHDGGETGASGGTGGAAGSGGAIIDSGADVSGSGGVQTGGSG
                     GAGGETGGVGGTGGTGTTGGTGGAAGTAGSGGTEVDSGNPLKSLGEPCGHHQECKADL
                     FCAARFGMLYSVCTRHCCSQEDCDLFSATCQPTNFGTTACFKAEPAEITDPCCNHMSN
                     CGTQTCAWSKNHWQCMDRSGKKNGEGCDNDEQCLSGICSSSLTGLAKRCMAPCCNDDN
                     CDGFDFCANVESQGQVRRQCVPFEVLVRLGGFDDRTPACCSDGDCSPGRCVPVSQQVL
                     YRQSTPFGPKVSKKTAVAMGCE"
     gene            5271..6833
                     /locus_tag="PK710_02500"
     CDS             5271..6833
                     /locus_tag="PK710_02500"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT08607.1"
                     /translation="MNAVMHSGTWARSAAGEGELRVCPACGTEYPPAGPQEGPVTFCP
                     NEGYAIVRKADVAAAHGDIMLGRTVAQRYTIVARLGTGSMGAVYRAWQDRMARWVALK
                     IMRYDRTVDAESRARFEREARSMSQLQSPHTVTVYDFGEAEDGSVYLAMELLEGESLG
                     QRLAREKRLPIRDAVHFVRGALTSLAEAHTKGIIHRDLKPDNLFLTEVPDVGGAPSRE
                     VCKILDFGIAKLIRDERAIDALETQAGTVFGTPRYMSPEQAQGLRLDARSDIYSLGVI
                     LYQMVTGIPPFDDEEAVVVMARHIKTKPVPPRDIAPEAEIPPSLERIILRALAKDPID
                     RPATAEAFLLALDAAERGFPASLPTVAPALRQRLASSASHPLPTLAPSVLRRRRRWLT
                     AILLFISAVLIVGVIVLWRDRMASKPDAASTFAGEEDIVFEEPTEPSPVVAQSANEPP
                     LSLDSLPIAEEHEKSKIDTSKRTSSKPKNSTEIPANSREPTAATPPTEETSPPPPPAP
                     TPTSSKRYGLFD"
     gene            6905..11842
                     /locus_tag="PK710_02505"
     CDS             6905..11842
                     /locus_tag="PK710_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08608.1"
                     /translation="MDPREVDAIVQRLVANPHDQDALTYAHQAGASDPRSYATLLERV
                     GRETPDPAYASHWLAEAANVWSTTLGDVHHAAEVLLLAVVKDPTQDIAADRLAQLYRD
                     KGDVKGLVALLEKRAKLLAPIAAQDDTFRTKLAAMHEELGTLWAEPPLSQPVRALDNF
                     KRAYETDPQSVFAIYSARELLKAQQQYAEAIPLFAMEQALVADHERKMALYRDQADVC
                     RLAQDYGALTSTLRQLRAYAPDDPGLMQEQATSVLERIRLGQPVGDDEILEGGQLFVA
                     LAESYDGEHGYSYSLAALEIDPSNDRAVQLTTYYGELLGRTAELPEWWKRYLSANPTG
                     VMAQEIGAKLQEVGQSIPAPGSMVREIMQPSTPSSAQPEPEPPAASGQPISSAESPAS
                     EAESIDAGASAAPIAPIETLSAAQLSALLDEASALASKHKTREALEKYMEVLRHDPIS
                     PEALSWVDEHLRAKRKFAELRDVYQAAARVPGLASDDRRKFLSKVANICEQQLRDVDG
                     AIQALKQAAQIDASARDNLRRLLEKGQRWDDLAMLLDQEVMDAPDSEAQIGLLKKLAA
                     MHESKRKDPVSAGEVWRRLAAIMVGDESPILDAVKLFEKGERFDLAAEVIADAVSAID
                     SEETRQSLLMKLGELRDKIDDKSGAGDAYAEAAAIIPNDTAWLAAQRSFEAAERWEDA
                     AHAVGQRAELNDDPKTKATLLAKEAEYLFHVGQGANAILRLEQATDLDPDNASYAAEL
                     EQRYLDADRADDLIRFLLARAEKLQSKDDRLAIRKRAAQMQREQFSDLEGTRESLLLV
                     LQDAEDLEALTWLADDARERGEYQNEASFLQRMVAVIDEPAEKVRLILRQASVLAEDC
                     NDIDGAVAAYQTVLERFDANNLGALEALAGLEERRDNPKGAVDALEKILACMQEPEQK
                     AEIGRRLADLYQGPLDDPRGAIRSLETVYAADPDDLDAVARLVELCESVEAWERTAEL
                     LAVLVEIEGDPEEASSLARRLAEILVDRLDNGQEALAVLEPLADEGDRDCRDAYVQLG
                     DKLGFKGIVAMKLRDWYATAPVADQHEAFRGAFERFIEMGRDQDAAQIAVELIRSRGA
                     TEEIIESLEEIAVRLKDLDSLATAHDYRVRDLSGEQRAEEFVRQAEVLLSAGVSTTEA
                     AQHGEQGLTSVAPADVEPLLERLSALVGEPNLVIDLYERQVGRCKNPADKLQALTRAA
                     QVAAAQGALDRAQAMFELALGGGVRDDVLEALEDAARSGDEATGTTQLRTALATSLAA
                     GAVGARDGGRTRSALLRRAALIAQRELGDTDRAFAWIGESIVTHVESDSLDALEALGE
                     EVGDLRRVEQTLGKALEQVFDGPLVRQLLTRRAALRRHHLNDAQGAADDLKRLHDLSP
                     HDKDVSDELHAILVELRDWRGVVQLLEDQILRGKDPALRAELARQAARLWEERLADPR
                     EAADAWRRVLRMKQGDKEAQEGLERAKTNMLKKPMSQLPPDPGSMDDLPPIPPAAPLA
                     GLDDDSLAPDTTPPEADASTALDYQEPLPPTQEIALPPESYPNDDQAYESQPAFEEMV
                     AEENMAADDSPLAQDTAHFDGQAYAAEPLQEPSEATPYLTDEYPASDMPYDPDANQEY
                     VPEELIESLDDAEDVDDEELIMEPARLAEDDEFER"
     gene            complement(11858..12748)
                     /locus_tag="PK710_02510"
     CDS             complement(11858..12748)
                     /locus_tag="PK710_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOT08609.1"
                     /translation="MLIPAALGPGDLIDVIAPSSPFDRTLAWKGLGWLGQRYRIRFDR
                     GIFQTQGYLAGSDDRRARELDRALRASDSKAVVAVRGGYGLHRIAPKMDWEAFRSQPK
                     WLVGFSDITVLHVEAMRAGVASMHAPMVAALGQSHEPTRRRWIEALEAPTQPRQWAGL
                     GCLRPGRVAGTSVGGNLTVLHACAAAGRLSLPDDAIIFLEDVGERPYRIDRMLTTLIT
                     AGVFEKARGMVLGEFTNCYPGTDRMEVMDVFCDLMSAFSFPTIIGFPMGHGELNDPLP
                     LGVPVELDAKEAGSMVSVFG"
     gene            12852..13487
                     /locus_tag="PK710_02515"
     CDS             12852..13487
                     /locus_tag="PK710_02515"
                     /inference="COORDINATES: protein motif:HMM:NF027592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HOT08610.1"
                     /translation="MPSSLYPGAMMMQRFLPLGIVLAFCLGCEAPPTESVESKPETIA
                     NAPRHSPQHEKAAGCADDHGMAATDDSLRKAKDPASGTEMTIVGSALSGLEKTAIPEL
                     LGKPETYAGKTIRLEGDVNAMCHHRRGWFAMQEPGDRTGAFVRVITNPAFLVPPGSIG
                     KKVRVEGKVDLIEESASAARHYAEGHQIGDPSAIQGPVKRVVLRASGAEFL"
     gene            13500..14132
                     /locus_tag="PK710_02520"
     CDS             13500..14132
                     /locus_tag="PK710_02520"
                     /inference="COORDINATES: protein motif:HMM:NF027675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HOT08611.1"
                     /translation="MRWRPLLLSLHRDIGFLCVGLTIVYAVSGIAVNHAHHWNYDRSE
                     THQSLWLGHPADLLQNASAERKQLVRSDPSQMTPQEEQTLVRAIGEKIGRPLPPRNVF
                     WRGPYEILAFWGQGDDDMTVYHVSSGMITHTLRKDRWIIRSFNALHLNERHGPWTFIA
                     DAFALLLCFLAVSGMLIVRGPRGIKGRGGIFLAIGVAIPILALLLGRFSP"
     gene            14383..17634
                     /locus_tag="PK710_02525"
     CDS             14383..17634
                     /locus_tag="PK710_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08612.1"
                     /translation="MLRLMSTLTWALALLLLLSAPASAADAAAPSPADGGTDGFSSLD
                     ADTEDKDAATAVPLAQQAADLRRILVSLRLVRDGKPPSDGYVEDLFPFNLSDEKAVDA
                     ARKDLRDDIAQRKTRAQQFEEQRQSSTPQKAKPHETIQDAGVSDALVDADGDDASSEQ
                     PEEIIDEFTVLMLELQVAEARLAFLDLPLDQRKILLEQDAQRKRADLEKERANAEREK
                     ALAEQKRAEAARQKALADAERARNEIDRIVANEHARMEGVRGAQAFYRRELAERRELW
                     AARAASLHERLNDLVERSEQVAQASGEANVLYDDIVHELATLRGIVREGLSAYRRVPT
                     TSRYETETAYDAIRGIVSEKDRSAIENNQQQLADSAADLERQARTLAWETLEASVAAE
                     RTLNAKRVELLDIVTPEKRSTVLGFGPEGIAQLRRELDRMALEGVWYRTHGEVRLRAL
                     KDLSTNPFWIGRVTWTAFLLLIVLGSVVYVRRKGAAVFESLRVVVVRSIRHPGIARPI
                     ATLIDAVVAVLSPLAMLLGVLAAQKALGDTATIIELAVPLRLLLWYSTYRLIIVGSHR
                     LLFRAISHENEDASESTNPRSELIARSVRMVARYVFGVAIILIVAAEVVDKGYLYHLV
                     LRFAWIGVFPIAFVLIRRWRKDIADAYLAHRSTGRLAELVRSTRHRWFGFFVAVAAFG
                     VVLGQASARTVRRFVLGFEQTRKALAFLFRRRLEKQAAEVAIATTDEPLPEELEACFS
                     EDPVQDEPFILSDVAGMDTFEKLLTSFSDGNKSTATLVVGETGFGKTSWLNIAKAKAK
                     MPVHRIVLERRILQEQELLAWLVQQLDLETAEPLSVSSIVESIEKRGKQMIQVDDGHL
                     LTLRGVGTSGAWNAFSQIVERSRAHAFWIVAINGFAFQHLAWARKGVDPFREIIHLKP
                     WSEARVAMLLQTRSKASGYEMVYDDLVVDKLEGVEGEAQLVSTQEGYARLIWDYADGC
                     PRVALHCWKDSLVADGPNRVRVRLFRRPHADELESLDDNERFVLASVIWHGSLTTEQA
                     VKSLNLPASVCGDAFGKLQDQGVLGQSHERYFVQTRWLCPVLRFLRRKHLIEE"
     gene            17666..18517
                     /locus_tag="PK710_02530"
     CDS             17666..18517
                     /locus_tag="PK710_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOT08613.1"
                     /translation="MDGMPTDAGAANVLDTIRVGGVLTGFIILVGTWFAVRIVTGTLE
                     RLGERFAAKRLVLAQIATLSRFVIYLLGLTIAIGASIQLSKEVVLALTGTAAVTVGFA
                     LKDLAASVLAGITIIIDRPFQVGDRVTFAGHYGEITQIGLRSVRLVTPDDNVITIPNN
                     KFLTEVVSSGNLGALDMLVEQDFYIGVDQDPALAKRLVAEASTSTRYAYLKKGWSVGI
                     NQVMIDNYYALRVRSNVYVLDVMYESALKTDVTERVMAAFREHGIHPPAVLHRHVDGG
                     YSPANAS"
     gene            complement(18654..18992)
                     /locus_tag="PK710_02535"
     CDS             complement(18654..18992)
                     /locus_tag="PK710_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HOT08614.1"
                     /translation="MAIDINKMNHRELTELIKQAEERKASIRKDGLEALKKQILSMIE
                     AEGYTFEEIFGTDAVTRKDSRQKRRKAAAKYRNPDDHSVTWSGRGKHPRWFNAALERG
                     ISQEDMLISR"
     gene            complement(19207..20352)
                     /locus_tag="PK710_02540"
     CDS             complement(19207..20352)
                     /locus_tag="PK710_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08615.1"
                     /translation="MTIRDILWGVKTRTLEQRLEALEKSGVRIIDPRQTYIGEDVPLE
                     RVAEGVVLHPGVRLHGSKTRLGARAEVGTEGPATLMDTVMDEEARVASGYVHGAVLLK
                     KASLGANAHVRAGTLLEEEASTAHAVGLKQTLLLSFVTLGSLINFCDCLMTGGTSRSD
                     HAEVGSGYIHFNFTPWGKHGDKATPSLIGSVPRGVFLRERRIFLGGTGGLVGPAEVGF
                     GAVAGAGQVVRHGIAEGHLVLQQTRPIDRAVEFGRLDAVMPRAEKNIRYIGHLHALRV
                     WYDKVRLFQCPPHRKAVFEAALELLDGCIEERCKQLGRFLEERGRVLPVISAVDPGPC
                     PLPLAADAVEDHIAWVRSLTEEQVMAGSQWLEGVVEGVSRPAISALA"
     gene            complement(20485..>21525)
                     /locus_tag="PK710_02545"
     CDS             complement(20485..>21525)
                     /locus_tag="PK710_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOT08616.1"
                     /translation="LLAVGGWVAGMLLERPIVWLIRRTARRAKIAHEAMASDLTKGLV
                     HWLVMLLVVLGTFPTLRLSATASIQISRVLITGIILVLMVSALRIIRISVQTIQLRHA
                     RDEDAARVRSIMTRLDALRRIAQVLVVLVGVALALMQFPTVRTVGLSLLGSAGIAGAI
                     LGFAAQKTFSNLFAGILISFSQPVRIGDTVIVEKEYGQIESIGSTHVVVRLWDNRRLV
                     VPVTYFLDNPFENWTKVSPDLRGTVFLHADFGVDVDAVRAELDRLLENEPLFDGQAKA
                     VLVTDVVEHAAVIRITVSARNAGDLFDLRCKIREKLLAFLYKDPSRLPRRRTEAVNMA
                     GPGMQEPPGLIS"
BASE COUNT         4802 a         6463 c         5877 g         4383 t
ORIGIN      
        1 gacgataaaa tggtgctcgt ccgattggcg gccaacggcc tagcccaatt cgaaaacatc
       61 atcgtaggcg gcaattccca tcaggccgaa ggagaccgat ggatcgacaa ttggccccat
      121 gaagggcgct tggcctggtc aggttccgaa tacgccgcct acttcggaca taccggcaac
      181 tggggctccc aaggaaatca tcaaggggac cgtctcgaaa tcatcgacgc caccggaaat
      241 ggaggagggg gaggatgggg ttggggatgc agccacagcc tcgatgtccg aattgcccac
      301 aatatccagc gtttcggccc cgtatgcctg tccgattgtt acccaggaaa gggcatctac
      361 ttcgaccacc acaccctcgt aagcgatgaa ccctccggca attgcatcgg tggcagcaac
      421 gctcgattgg ggggactggc cgccgtggat ggtggattct ggttgaccta ttcgtcttcc
      481 gaaggacgcg cttccagtga tatcgccctc gtgcatatcg acgatgcagg ggcggtgtct
      541 ccccctcaat ggcttactaa cacggattcg gagcaagaaa aattcgccta cctcgcacgt
      601 tacggttcgg gcttgctcgc ggctttcgag tccgggggac aacgccaatt cgtcgttctc
      661 gacgcttcgg caaccgtaac ggaaggacct gtgccgttgg atgtaccgct tcccacgggc
      721 gcggatatgg tgacgtttcc caatggcgat gtgggctggg gatccacgga cggttacggt
      781 ttgacggtcg ttcgtgtgaa aagatgccaa tgatgcgctg gggctcgaac gttttttcgg
      841 aagaccgtgg ggaggcctgc ctcgctagtg tcgatgctca tatctgtttg acagaccgac
      901 gccttgcttt ttgcatcccc cacgccacca catcacgcaa ctcttcggca cgtaatgcgt
      961 aagcaacgat gccgaacacc gctacccccg cggttgcggc gatgctgcca cggatgagca
     1021 ggtgagtatc ggtcaacagc gtgcgagccg ctaatccgca agcgataccc gcgaataccg
     1081 caggacagaa gcgaagcaaa aaacgtccgt aatgatcggc cacgttggga aagtaggcgc
     1141 gcaaacgaat agcaagcacc accgtatata cgctgatcgc gatggcgctc gaagccgcaa
     1201 gaccgatggc gccatagccc gccatggccc agtacatcgg gtatgccaac acgacgatcg
     1261 acgtgccaag aagcgtcggt tcccacgttt ttccaagcgc ataataccct cgggacaata
     1321 cggtttgtgc cgcccaggcc cacaatccca cggacatgat gcccagcacc caccccatcg
     1381 tcacgtattg gtcatggggg atttttcttc cataaatgac ggttgcgatt tcttgtcctg
     1441 ccgtggtcaa tacgacttgt gctgccaggg caaggacgag catgcggcgc acggcagtcg
     1501 acaacgttgt gtaggcttcg gcgtgagagt tttcggctac caatcggcta agggtgggga
     1561 aagcggctac cccagccgcc aggccaaata cgcccatcgg cactttcagc agcgtcttgc
     1621 tgtagtgcaa ggtggcgatg gtgctggctc ccacgatcga accgaagcgt cccaggagcc
     1681 aatcatccac gacgacgatg gaccaaccga gcatgatggg tagggagcgg aatagatagg
     1741 tgcgaagatc cgggtgtttg atttggaaaa tgggtcgcca gctcaagcca acgtgcaagc
     1801 agccgatgag gggaagggcg aacgggccga ggatgcttcc cactagaacg ccccatgcaa
     1861 atccatacgc tcccgcttcg gttccgccga cgaggcctcc tacgacgata gcgcttgtat
     1921 aaagcagggg agcgagtgct ggaagactgt gtttgtcgcg ggcttggagt gcggcgctca
     1981 acagtccgcc caccagatgg aatacttgag cgggtaaaat gatgcgggtg aggttgacca
     2041 attcgcttcg ttggtactca tcgaatcctg gagccacgag cgagacgagc cacggtacgg
     2101 caatccacag ggctacggtg gcaattccaa gcaaggcgag aaggaagttg gcgatgatgc
     2161 tgaaggtttc ccaaccacgc ttttcctcgc ctttggcaag gtaaccgctg aagatcggga
     2221 tgaacacgat cgacaaggcg ccgcctgcga gcaggtagtt caagaaatcc gggacgatga
     2281 acgaggcgag aaagacatcg gcttcttttc cgcctccgag aatgcgaccg aggacggctt
     2341 cgcgaaccaa tccgatgatg cgagaaagga aaatggaagc accccagatc agggaagcga
     2401 taccgattcg acgggttacg gaaggtggca tgatgggacg actggttctg tcgtgccaga
     2461 aatggatgga gggagccaag ttgcgagcaa ctctcagaag ccgacaccca ccgtggcggc
     2521 aaggaccagc catggtttga ggtcgggagc attgtcgctt cggatggccg gaagcatgcc
     2581 gatggcgctg attccggcgc gagtatcggt ggattccacg ggaatgcggt attcgatgcc
     2641 ggcgcgtacc aagccaatgc ccagggattg ggcgacaacg cctgtttcct cggattgttt
     2701 ttcatacact cgcgaaagac ccgccccggc ccccagcacc ggtcgccaac tgtggcgacc
     2761 acccagatcg agcgtcaatt cgccagtata atgggccacg ccgtggttgg agcccacgcg
     2821 aagccaagag cctgaaaaac ggaatcccgc cggtccccgg cccacgtcca acgccgtcca
     2881 caatccgggg gtaacctcat cctctcggat tctgcccgcc ccaccaatgt ggaagcgaaa
     2941 ggtggaaagc tcgctttttc gccagctttg catctcgtac ggatcgtccg gaggatcggc
     3001 aacttgggaa tacctcggtt ccggctctcg gacgggatag ggtggggccg cataggtctg
     3061 tgcccacgct ggcatggcgg aaaaaaaggt gaataagtag aatttccaag ctgagcgtcg
     3121 catggtgact ccttcttttg cacggaacgc cacagccccg cctcacctta cgctatgatg
     3181 tcctgtgatg ttcaaaacgg tgtcttgggt tggttcgtgg atgctggtga tggcaacttc
     3241 gctggcgaca tcgctcgtct tggcgcagcc ctcgaaacta tcgaggacgg cgattgggca
     3301 aacctcgcag gcgagcgcgg agcagcactt tggcaaggct cgggagtatt acgccaatgg
     3361 ccgctatcgg gaggcgatcg agcaactcga gatcgcattg cgaatcgatc caaaaggggc
     3421 ggagttgctc tacaacctcg gggtcattca cgagaagctg caggaactcg atccagccat
     3481 cgattattac aagcgatatg tgttgatggt gggagatccc gaagagcagc agcgggtcaa
     3541 gacaattatt gcgaggctcg agaaagccag agacgagctt ggcaacgaca aggaacaacc
     3601 ggagttcgcc gaggaaaaac ctattcctgt gaaagaaccg gagcgaacca aaggccggtt
     3661 cgacggttgg gtcatcggca ccgggatcct ggcggcagtt ggtgtggcag gaggaaccta
     3721 tttcgggctc gaggcgttga agagtcgaaa cgaggacccg cccacgaccg gaaaagggac
     3781 ttcctatgcg gatttggagg ataacgcgga ccgtgccaaa cagatggcct tgtttgcgga
     3841 tattgggttc ggagtgggag tcgtagcggg tactgccgcg atgttattgt actttatgcg
     3901 agatggggaa ccagagcagg ccgccgtggg cgcgtgggaa ccaggtgttg ccgtcgtgcc
     3961 gggaggaatg gcggcatcga tgaaattggg gttctaatgg aacggcgagc gagctgcact
     4021 tgcgctacga tgagcaaggc atcaaggctt tttcgttggg atcgggaatg gcaatgaacc
     4081 ggcattggct acttgggctc ggagtgatga taggggtagg ggcctgccaa ttggtggtgg
     4141 gtgaaacggg gctggggacg gttcgttgtc aggatgaggg agcgatgggg ccgccggcct
     4201 gcgcgaaagg gcagacgtgt gtgaagggga cgtgtgtggt ggcggtgaat atcggccatg
     4261 atgggggaga gacaggcgca tcggggggaa cgggtggtgc ggcgggcagt ggaggagcca
     4321 ttatcgatag tggagcagac gttagtggtt cgggtggtgt gcaaaccggt ggctcgggag
     4381 gtgcgggtgg agaaacggga ggggttgggg gaacgggtgg gactggaaca accgggggta
     4441 ctgggggtgc ggcgggaact gcgggctcgg gagggacaga agtcgattcg ggcaacccat
     4501 tgaaatcatt gggagaacct tgcggccatc atcaagagtg taaggcggac ttgttctgtg
     4561 ccgcgcgttt tggaatgctc tattctgtat gcacgcgaca ttgttgctct caagaggatt
     4621 gcgacttgtt cagtgctaca tgccagccta ccaatttcgg cactactgca tgtttcaaag
     4681 cagagcctgc ggagataacg gacccgtgct gtaaccacat gtccaactgc gggactcaaa
     4741 catgtgcatg gtccaagaat cactggcagt gtatggatcg ttcgggaaag aaaaacggtg
     4801 aggggtgcga taacgacgag cagtgtttga gcggtatttg ttctagttcg ctaacaggtc
     4861 tagcaaaacg ctgcatggcc ccctgctgta acgatgacaa ttgtgatggg tttgattttt
     4921 gcgccaatgt tgaatcgcaa ggccaagtgc ggcgtcaatg cgttccgttt gaggtattag
     4981 tacgattggg tggatttgat gaccgtacgc ccgcttgttg ctcggacggc gactgttcgc
     5041 cgggccgttg tgtgcccgta agtcaacaag ttttgtaccg ccagtccaca ccatttggcc
     5101 cgaaagtgtc aaaaaaaacc gctgtcgcga tggggtgtga gtagccgcgg ggttgaggta
     5161 taaggtatct tgcacaacga ccctttcggg gtcaggatgc aacgcgtggt ctatttggga
     5221 ctgcgcggtc gccgaggccg ggaccatcgc ctttggttgg gtttgccgtc gtgaacgctg
     5281 tcatgcactc cggaacctgg gcccgctccg ccgcgggaga aggggaactt cgtgtgtgcc
     5341 cggcgtgcgg aacggaatac cccccggctg ggccgcaaga aggaccggtt acgttctgtc
     5401 ccaacgaggg ctacgcgatt gtgcgcaaag ccgatgtggc tgctgcgcat ggcgatatca
     5461 tgctcggccg gaccgtggcg cagcgctata ccatcgtggc gcgtttggga accggatcga
     5521 tgggggctgt gtatcgcgca tggcaagatc ggatggcccg gtgggtagcg ctgaaaatca
     5581 tgcgttacga ccgcacggtc gacgccgaat ctcgcgcgcg tttcgaacgt gaggcgcgat
     5641 ccatgtccca gcttcaatcc ccgcatacgg tcaccgtcta tgacttcggg gaagcggaag
     5701 atgggtccgt atatctcgcg atggaacttc tcgaaggcga gtcgttgggg cagcggttag
     5761 cgagggaaaa gaggctgccg attcgcgatg ccgttcattt cgtgcgtggc gcgctgacat
     5821 cgctcgccga agcccatacc aagggaatca tccatcgcga cctcaaaccg gacaatcttt
     5881 ttctgacgga agtgccggat gtggggggag cgcctagccg cgaagtctgc aaaatcctcg
     5941 actttggaat tgccaaactc attcgcgacg aacgggccat cgatgctctc gaaacccagg
     6001 ccgggaccgt gtttggaacg ccgcgctaca tgtcccccga gcaagcccaa ggtttgcgtc
     6061 tcgatgcccg aagcgacatc tattcgctcg gcgtgattct ctatcaaatg gtcacgggaa
     6121 ttccgccttt cgatgacgaa gaggcggtcg tggtgatggc gcgtcatatc aaaaccaaac
     6181 ccgtgcctcc tcgtgatatc gcgcccgaag cggagattcc gccaagcctc gaacgcatca
     6241 ttttacgtgc gttggcgaaa gatcccatcg accgaccggc gacagccgag gcctttttgc
     6301 tcgccctcga tgcggcggaa cgcggttttc ccgcttcgct gcccaccgtg gcccccgccc
     6361 tgcgtcaacg gctggcgtca agcgcaagcc atccgctgcc aacgttggct ccaagcgtgt
     6421 tgcgtcgtcg acgacgctgg ttgaccgcga ttttactttt catctcggcg gttttgatcg
     6481 tgggggtcat cgtgctgtgg cgggaccgaa tggcttccaa gcccgatgcg gcatcgacgt
     6541 ttgcggggga agaggatatc gtcttcgagg aaccgaccga accgtcaccg gtggtggcgc
     6601 aatccgcgaa tgaacccccg ctttcactcg attcacttcc aattgcggaa gaacatgaaa
     6661 aatcaaaaat agatacctcc aaacgaacct cctcgaaacc gaaaaattcc actgaaatac
     6721 cggcaaattc gagagagccg acagccgcaa cgcctcctac cgaagagacc tccccacctc
     6781 cgccccccgc tccaaccccc acctcttcga agaggtacgg actcttcgac tgagcctctc
     6841 cttcgaattg ccgaaacggt tcgttgccgc atgctttccg cgcgtggtag cgtcccgtcc
     6901 cgacatggat cctcgcgaag tcgacgcaat cgttcaacgg ctcgttgcca atcctcatga
     6961 tcaagatgct ctgacctatg cgcatcaagc aggtgcaagc gatccacgat cctatgcaac
     7021 cttgctcgag cgtgtggggc gggagacgcc ggaccctgcc tatgctagcc attggcttgc
     7081 cgaggcggcg aacgtttggt ccacgacgtt aggggatgtg catcatgcgg ccgaggtgtt
     7141 gttgctcgcc gtggtcaagg atcccacgca agacatcgcg gccgatcgat tggcgcaact
     7201 ttatcgggac aagggcgacg tcaaagggtt ggtcgctttg ttggagaagc gcgccaagtt
     7261 gctcgctccc attgccgccc aagacgatac gtttcgcacc aagcttgcgg cgatgcatga
     7321 agagcttgga accctctggg ccgaaccacc tttgtcacag cccgtgcgcg cgctcgacaa
     7381 cttcaaacgc gcctatgaaa ccgatcccca atcggtgttt gcgatctact ccgcgcgaga
     7441 attgctcaaa gcgcaacagc agtatgcaga agccatcccc ttgtttgcca tggagcaagc
     7501 attggttgcg gaccacgaaa ggaaaatggc tctgtaccga gatcaagcgg atgtctgcag
     7561 actcgcgcag gattatggcg cgctgacgtc aaccttgcgt cagcttcgcg cctacgcgcc
     7621 cgacgacccg gggctcatgc aagaacaagc tacctcggtg ctcgaaagga ttcgtcttgg
     7681 tcagcccgtg ggagacgacg agattcttga aggcgggcaa ttgttcgttg ctttggcgga
     7741 aagctatgat ggtgagcatg gctactccta ttcgctcgcc gcgttggaga tcgatccgtc
     7801 caacgatcgc gccgtgcaat tgaccaccta ttatggggag ctattgggac gaacggcgga
     7861 attgcccgaa tggtggaaac ggtatctttc cgccaatccg accggcgtca tggcgcagga
     7921 aatcggtgcg aagttgcaag aggtgggaca atcgattccg gctccaggat cgatggttcg
     7981 cgaaatcatg caaccgtcta cgccatcatc ggcgcagcct gaacccgagc ctcccgcagc
     8041 ttccggacaa cccatatcgt cggcggaatc accggcatcc gaagcggaat ctatcgatgc
     8101 gggagcatct gccgctccca tcgcccccat cgaaacgctc agtgcagccc aactctccgc
     8161 gttgctcgat gaggcgagtg ctttggcttc caagcacaaa acgagagagg ctctcgaaaa
     8221 gtacatggaa gtgcttcgcc acgatcccat atctcccgag gcgttgagtt gggtcgacga
     8281 acacttgcgt gcaaagcgta agttcgcgga actacgtgat gtttaccagg cggccgctag
     8341 ggttcccgga ttggcttccg acgatcgtcg aaaattcttg tccaaggtcg ccaacatctg
     8401 tgaacaacag ctccgcgatg tggatggggc catccaagcc ctcaagcagg ccgcgcaaat
     8461 cgacgccagc gctcgcgata atcttcgtcg gcttctcgaa aaaggacagc gctgggacga
     8521 cttggccatg ttgctcgacc aggaagtcat ggacgcgcct gattccgaag cacaaattgg
     8581 cctgctgaaa aaactcgccg ccatgcatga aagcaaacgc aaggaccccg tttccgcggg
     8641 cgaagtgtgg agacggcttg ccgcgatcat ggtcggcgat gagtcaccga tcctcgatgc
     8701 cgtcaagctt ttcgaaaaag gagaacgttt cgatctcgcc gctgaagtca ttgcggatgc
     8761 ggtgagcgct atcgattcgg aggagacgcg acaatccttg ttgatgaaac tgggggagtt
     8821 gcgcgacaaa atcgatgata aaagcggtgc gggcgatgcg tatgccgaag ccgccgccat
     8881 cattcccaac gacacggctt ggctagcagc gcaacgttct ttcgaagctg ccgagcgatg
     8941 ggaagatgcc gcccacgccg tgggacagcg cgcggaactc aacgatgacc ccaaaacaaa
     9001 agctaccttg ctcgcaaagg aagctgaata cctgttccac gtaggacaag gagccaacgc
     9061 gatccttcgt ctcgaacaag ccaccgatct cgatccggat aatgcatcat acgcggcaga
     9121 gctggagcag cgttatctcg acgccgatcg tgccgacgat ctcatccgat ttcttcttgc
     9181 acgagcagaa aaactgcaga gcaaagacga ccgccttgcg atacgaaaac gagccgcgca
     9241 aatgcaacgc gagcagtttt ctgacttgga ggggacgaga gaatcgctgt tgcttgtctt
     9301 gcaagatgcg gaagatctcg aggctctcac ctggcttgcg gacgatgctc gtgaacgcgg
     9361 tgagtaccaa aacgaagctt ctttcttgca gcgcatggtg gccgtcatcg atgagcccgc
     9421 ggaaaaagta cgcctcatcc ttcgtcaagc ttccgtgctc gccgaagatt gcaacgacat
     9481 cgatggcgct gttgccgcgt atcaaaccgt tctcgagcgc ttcgacgcca acaacctcgg
     9541 tgccctcgaa gccctcgctg gtctggaaga gcgccgcgat aacccgaaag gggccgtgga
     9601 tgcgctcgaa aaaattctcg catgcatgca agaaccagaa caaaaagcgg aaatcggacg
     9661 tcgcttggcc gacctatacc aaggtcccct cgatgatccc cgtggcgcca ttcgttccct
     9721 agaaaccgta tatgctgcgg atcccgatga cctggatgcc gtcgcccgtt tggtagaact
     9781 ctgcgaaagc gtcgaagcct gggagcgaac cgccgaactg ctcgcggttc tcgtagaaat
     9841 cgaaggagac cccgaggaag cttctagctt ggctcgccgt ctcgccgaaa tcctcgtgga
     9901 ccgattggac aacggccaag aggccctcgc ggtcctcgaa cctcttgccg atgagggaga
     9961 tcgagattgc cgagatgcct acgtacagct cggtgacaag cttggcttca aaggcatcgt
    10021 cgccatgaaa ctaagggatt ggtacgccac cgctcccgtc gctgaccagc atgaggcttt
    10081 ccggggcgcg ttcgaacgat tcatcgaaat gggacgtgac caagatgctg cccaaatcgc
    10141 cgtcgaactc atccgctctc ggggagcgac cgaagagatc atcgaatcgc tcgaggagat
    10201 cgccgttcgc ctgaaagacc tggattcctt ggctaccgct catgattatc gtgtccggga
    10261 tttatccggt gaacaacgtg ccgaagaatt cgttcgtcaa gccgaggttc tccttagtgc
    10321 gggcgtttcc accacggaag ccgcccagca cggtgaacaa ggattgacca gtgtggctcc
    10381 cgccgatgtc gagccgctgc tcgaacgctt gtcggcgctc gtgggcgaac cgaatctcgt
    10441 catcgacttg tacgaacgac aagtcggacg atgcaaaaac cccgcggaca aactgcaagc
    10501 cttgacgcga gccgcgcaag ttgcggcggc acagggggcc ctcgaccgcg cacaggctat
    10561 gttcgaattg gctcttggcg gcggagtccg cgacgatgtg ctcgaagcgc tcgaagacgc
    10621 agctcggtct ggcgatgaag ccacgggaac cacgcagctt cgtacagcct tggctacctc
    10681 actggcggca ggagcggtgg gcgcacgcga tggcggaaga acccgaagcg ctctgctgcg
    10741 tcgcgctgcc ttgatcgctc agcgtgaatt gggagatacg gaccgcgcct tcgcatggat
    10801 cggtgagtcc atcgttaccc atgtggaatc cgattcgctc gatgccctcg aggccttggg
    10861 tgaagaggtc ggagacctgc gccgtgtcga acaaaccctt ggaaaagcct tggaacaggt
    10921 cttcgatggt cccctggttc gccaactgct cacacgtcgt gccgcgctgc ggcgacatca
    10981 cttgaatgat gcgcaagggg ccgccgatga tctgaaaagg cttcacgatc tttctcccca
    11041 tgacaaagac gtttccgacg aactgcacgc aattctcgtc gaactccgag attggcgtgg
    11101 cgttgtccaa ttgctcgaag accaaatcct acgaggaaaa gaccccgccc tgcgggcaga
    11161 actggcaaga caagctgctc gtctttggga agaacgcttg gccgacccgc gggaagctgc
    11221 cgatgcctgg cgacgcgtgt tgcgcatgaa acaaggagac aaagaagcac aagaaggatt
    11281 ggagcgcgca aaaaccaata tgctcaaaaa acccatgtcc cagctccctc cggacccggg
    11341 aagcatggat gacctgcctc ctatcccccc tgccgctcct ctcgccgggc tcgatgacga
    11401 tagcctggct cccgatacta cgcctccgga agctgacgcc tccaccgccc tggactacca
    11461 agaaccactg ccgccaaccc aagaaatcgc tctgccgccg gaatcgtacc ccaacgacga
    11521 ccaagcttat gagtcgcaac ccgctttcga agaaatggtt gccgaagaga atatggccgc
    11581 cgatgattct ccgcttgcgc aggacactgc tcacttcgat ggacaggcat acgccgcgga
    11641 acctcttcaa gaaccttctg aggcaacccc ttacttgacc gatgaatacc ccgcctcgga
    11701 catgccttac gatccagacg caaatcagga gtacgttcct gaagaactca tcgaatcgct
    11761 cgacgatgcc gaggatgtcg acgacgaaga gttgatcatg gaaccagcac gattggcgga
    11821 agacgacgaa ttcgaacgct gatatccgcc ttattcatta tccaaatacg gataccatag
    11881 aacccgcttc tttggcgtcg agttccacgg gaactccaag gggaagcgga tcgttcaatt
    11941 caccatgacc cattggaaat ccaatgatcg tgggaaatga aaaggcagac atcaaatcgc
    12001 aaaaaacgtc catcacttcc attctatccg ttccgggata acagttcgtg aactcgccca
    12061 gaaccattcc cctcgctttt tcaaacactc ccgccgtgat gagcgtggtg agcatgcgat
    12121 cgatgcgata gggacgctct cccacgtctt ctagaaaaat aatggcatca tccggcagag
    12181 aaagtcgccc ggcggccgca caggcgtgga gcaccgtgag attgccccca acactcgttc
    12241 cggctacgcg ccccggccgc aaacagccca atccggccca ttgccttggc tgcgtcggcg
    12301 cttcgagtgc ctcgatccat cggcgacgcg tcggttcatg actctgaccc aaggcggcta
    12361 ccatcggcgc atgcatgctc gcgactcctg cccgcatggc ttctacatga agcaccgtga
    12421 tatccgaaaa acccaccagc cacttgggtt gactgcgaaa cgcttcccaa tccatcttgg
    12481 gcgcgattcg atgcaatcca tatcccccac gcaccgccac caccgctttt gaatcgctcg
    12541 cccgtagtgc acgatccaat tctctcgccc ttcgatcatc cgagcccgcc aaataccctt
    12601 gcgtttgaaa tatcccccga tcgaatcgaa tgcgataccg ctgccctaac caccccaacc
    12661 ccttccatgc caacgtcctg tcgaaggggc tggagggcgc aatgacatca atgagatcgc
    12721 cgggtccaag agctgccgga atcaacatga cacgcactct gccatagctc gcccactcct
    12781 acgaccaacc agatcgccag ccgcgctttc atgaaggctg cggcgttgca ttgacgctca
    12841 ctcacgctag gatgccatcg tccttgtacc cgggagccat gatgatgcag cgtttcttgc
    12901 ctttggggat tgtgctcgcc ttctgccttg gatgcgaagc tccccccacg gagtcggtag
    12961 aatccaaacc agaaaccatc gccaacgctc cacgccattc tccgcaacac gaaaaggcag
    13021 caggttgcgc cgatgaccat ggcatggccg ccactgacga ctctttgcga aaagccaaag
    13081 accccgcctc gggcaccgag atgaccatcg tcggaagcgc cttgtccggc ttggaaaaaa
    13141 ccgcgatccc tgagctgctc ggcaaaccgg aaacctacgc cggaaaaacc atacgcctcg
    13201 aaggcgacgt caatgccatg tgtcatcatc gtcgcggctg gttcgccatg caagaaccag
    13261 gggatagaac aggagccttc gtgcgcgtca tcaccaaccc ggcctttctc gttcctcctg
    13321 gttccatcgg caaaaaagtg agggtcgaag gaaaagtgga tctcatcgaa gagtccgcgt
    13381 ccgccgcacg ccattatgcc gaaggacacc aaatcggtga cccctcggca atccaagggc
    13441 ccgtaaaacg cgtggtccta cgagcctcgg gcgctgaatt cctctgatcc cccatcacca
    13501 tgcgctggcg tcccctgttg ctttcccttc accgcgacat cggctttctg tgtgtcggcc
    13561 tcaccatcgt ctatgccgtc tcgggaatcg ccgtcaatca tgcccaccat tggaactatg
    13621 accgttccga aacccaccaa tccctgtggc tcggacatcc cgcggacctg ctacaaaacg
    13681 cctccgccga acgaaaacaa ctcgttcgct ccgacccatc gcaaatgaca ccacaggaag
    13741 aacagaccct cgttcgcgcc atcggcgaaa aaatcggccg accactgccc cctcgtaacg
    13801 tgttctggcg cggaccgtac gaaatcctgg ccttctgggg acaaggggac gacgatatga
    13861 ccgtctacca cgtctcgtcg gggatgatca cccatacgct gcgcaaagac cgctggatca
    13921 ttcgctcctt caacgctttg catctcaatg aacggcatgg tccctggacg ttcatcgccg
    13981 atgcttttgc cctgctcctc tgctttcttg cagtctccgg catgctcatc gtgcgaggcc
    14041 cccgcggcat caagggcagg ggagggatct tcttggccat cggagtggca attcccattc
    14101 tcgcactcct cctggggcgg ttctccccgt gaaccatgcc caggaactgc aataatagca
    14161 cgtaaaatca atcggttaca agctgatggc ccattggctc cggctccgac ccaccctgct
    14221 ttcccttttc cacgtctttc aacgtcattg atttcccgtt ttgccatcgg cttgctccga
    14281 gttcgcccgt ttatgctcta agtattgata attattattg ttattcggat ctccgccacc
    14341 ccccacgttg ccaacccctt cccacaatga tacgtcgcgc ccatgcttcg cctcatgtcc
    14401 accctgacct gggccctcgc tttgctcctg cttctgtcag cacccgcatc agccgccgat
    14461 gcggcagcgc cctcgcctgc ggatggcggc accgatggct tctcttccct cgacgccgac
    14521 acggaggata aagacgcggc gaccgcggtg ccccttgccc aacaagccgc cgatttacgc
    14581 cggattctcg tttccttacg actcgtccgg gatggcaaac ccccttccga tgggtatgtc
    14641 gaagaccttt tccccttcaa tctcagcgac gaaaaagcgg tcgacgccgc gcgcaaagac
    14701 ctgcgcgatg atatcgctca acgaaaaact cgcgcgcagc aattcgagga gcagcggcaa
    14761 tcctccacac cgcaaaaagc gaaaccccac gagaccatcc aagacgctgg cgtgtccgat
    14821 gccctcgtcg atgccgatgg cgacgacgcc tcttccgaac aaccggaaga aatcatcgac
    14881 gaatttaccg ttctgatgct cgaactgcag gtagcggaag ctcgtctcgc ttttctcgat
    14941 cttcctttgg atcaacgaaa aatcctcctg gaacaggacg cacaacgcaa acgggcggat
    15001 cttgaaaaag aaagagccaa tgccgaacgc gagaaagccc tcgccgaaca aaaacgagcc
    15061 gaggccgctc gtcaaaaagc cctcgccgat gccgaacgcg ctcgcaatga aatcgaccgt
    15121 atcgtcgcca acgaacacgc acgcatggaa ggcgtgagag gcgcacaagc cttttatcgt
    15181 cgtgaactcg ccgagcgacg agaactctgg gctgctcgtg cggcttcgtt gcacgaacgt
    15241 ctcaatgact tggtcgagcg ctcagaacag gtggcccagg cgtctgggga ggcaaatgtc
    15301 ctgtacgacg atatcgtcca tgaactcgct accttacggg ggatcgtccg ggaaggattg
    15361 tcagcttatc gacgtgtccc caccacatcc cgctacgaaa cggagactgc ttacgacgcc
    15421 attcgtggaa tagtttccga aaaagaccgc tcggccatcg aaaacaacca gcaacaactt
    15481 gccgattccg ccgccgatct cgaacgtcaa gcacgaacgc tcgcttggga aaccctcgag
    15541 gcatccgtgg cggccgaacg aaccctcaat gccaagcgcg tggagttgct cgatatcgtc
    15601 acgccggaaa aaagaagtac cgtactaggc tttggccccg aaggcatcgc acaactccgt
    15661 cgtgaactcg accgaatggc cctcgaaggc gtgtggtatc gaacgcatgg cgaagttcga
    15721 cttcgagcgc tcaaagattt gtccaccaac cctttttgga tcggacgcgt gacgtggacc
    15781 gcgttcttat tgctcatcgt acttggttcc gtggtctatg tacgacgaaa gggtgctgcg
    15841 gttttcgaaa gtttgcgcgt cgtcgttgtg cgatccattc gccatcctgg catcgctcga
    15901 ccgattgcca cactcatcga tgccgtcgtg gccgtcctat cgccccttgc catgttgctt
    15961 ggcgtactcg ctgcccaaaa agcgcttggt gataccgcta ccatcattga actcgccgtc
    16021 ccgttgcgat tgctgctctg gtattccacc taccgactca tcatcgtagg ttcccaccgt
    16081 ctcctgttca gagccatcag ccacgaaaac gaggatgcct ccgaatcgac caatccgcgc
    16141 agcgaactca tcgctcggtc ggtgcgcatg gtcgctcgct atgtcttcgg ggtcgccatc
    16201 atcctcatcg tagccgccga agtcgtggac aaaggctatt tgtatcatct cgttcttcgt
    16261 ttcgcctgga tcggcgtatt tcccatcgcc ttcgtgctca ttcgtcgttg gcgcaaagac
    16321 attgccgatg cctatcttgc ccatcgttcc accgggcgtt tggctgaact cgtgcgttcc
    16381 acacgccatc ggtggttcgg atttttcgtg gcggtagcgg ctttcggtgt ggtgttggga
    16441 caagcttccg cgcggaccgt acgacgtttc gttcttggat ttgaacagac tcgaaaagca
    16501 ctcgcgtttc tatttcgtcg acgactcgaa aagcaagcag ccgaggtcgc aatcgctacc
    16561 accgacgaac cgttgcccga agaactcgag gcttgttttt cggaagatcc cgttcaagac
    16621 gagccgttta tcctgtccga tgtcgcggga atggatacgt tcgaaaagct gctcacgagc
    16681 ttttccgacg gcaacaaatc gaccgcgacc ctggtggtgg gagaaacggg atttggaaaa
    16741 acatcgtggc tcaatatcgc caaagccaaa gcgaagatgc ccgtgcatcg catcgtgctg
    16801 gagcgcagaa ttctgcaaga acaagaactc ctcgcgtggc tcgtgcaaca gctcgatctc
    16861 gagactgctg agcctttgag cgtctcttcg attgtggagt ccatcgaaaa acgcggaaag
    16921 caaatgattc aagtggacga tggacacctt ctcacgctgc gcggtgtggg aacctcaggc
    16981 gcttggaacg ccttctccca gatcgtcgaa cgttcgcgcg cccacgcgtt ttggattgtc
    17041 gccatcaacg gattcgcctt ccaacacctt gcctgggctc gaaaaggcgt cgaccccttc
    17101 cgggaaatca tccacctgaa accttggtcc gaagcccgcg tcgccatgct gctgcaaacc
    17161 agatccaagg cgagcggcta cgaaatggtt tacgacgatc tggtggtcga taaactcgaa
    17221 ggcgtcgaag gagaagccca actcgtcagc acccaggaag ggtatgcccg cttgatctgg
    17281 gattatgccg acggctgtcc gcgcgtcgct cttcattgct ggaaagactc cctcgtggcc
    17341 gatggcccca accgcgtgcg cgtccgcttg ttccgtcgcc cccacgccga tgaactcgag
    17401 agcctcgatg acaacgagcg attcgttctc gccagcgtca tctggcatgg gtcgctaacc
    17461 acggaacaag ccgtgaagtc cttgaatttg cctgcttccg tttgtggcga cgcgttcggc
    17521 aagctgcaag atcaaggcgt tctcggccag agtcacgaac ggtatttcgt gcagacacga
    17581 tggctatgcc ccgtgctgcg cttccttcga agaaaacacc tcatcgagga atgatgtacc
    17641 caccggatac tagagaggtc tgaacatgga cggaatgccc acggacgcgg gagcggccaa
    17701 tgtattggat acgatccgag ttggcggcgt acttaccgga ttcatcatcc ttgtcggaac
    17761 ctggtttgcc gttcgaatcg tgaccggaac cctggaaaga ctaggggaac gcttcgctgc
    17821 caaacgtttg gtgctcgcgc aaatcgctac cctcagccgc ttcgtcatct acttattggg
    17881 tctgaccatc gccattggcg catcgattca gctttccaaa gaagtcgtcc tcgcattgac
    17941 cggaaccgcc gccgttaccg tcggcttcgc cctcaaagac ctggctgcct ctgtcctcgc
    18001 gggaattacc atcattatcg accggccctt tcaagtagga gaccgcgtca catttgccgg
    18061 ccattatggc gaaattaccc aaatcggttt gcgctccgtt cgcctcgtca cgcccgatga
    18121 taacgtcatc accattccca ataataaatt cctcaccgaa gtcgtatcct ccggtaacct
    18181 cggcgccctc gatatgctcg tcgaacaaga cttttatatc ggcgtggatc aagacccggc
    18241 tctcgccaaa cgcctcgtgg cggaagccag caccagcacc cgttacgcat atctaaaaaa
    18301 aggttggagc gtgggaatca accaggtcat gatcgacaat tattacgccc tgcgcgtgcg
    18361 atccaacgtc tatgtgctcg atgtcatgta cgagagcgcc ttgaaaaccg atgttaccga
    18421 aagagtcatg gccgcgtttc gagaacatgg tattcaccca cccgccgtgc tccaccgtca
    18481 tgtcgacggt ggctattccc ctgccaacgc ttcatgaacg ctctcggctc cgagagaaat
    18541 acgcgatttg aaaataggag gatggaatgc ttcgaggaaa cgaggtcgat atttcttagc
    18601 tcgtatcctt gctcggattt tttataagta tttgaatttg ttgaaaattt atttcatctg
    18661 gagatcagca tatcctcttg ggaaattcct ctctccaacg cggcattgaa ccaacgcgga
    18721 tgtttgccac gccccgacca ggtgacagaa tgatcgtccg gattgcgata cttggcggct
    18781 gctttgcgac gcttctgccg tgagtctttt cgagtgactg catctgtccc aaaaatttct
    18841 tcgaacgtat atccttcggc ctcgatcatc gaaagaatct gtttcttgag cgcttcgaga
    18901 ccgtctttac gaatggaggc cttccgctcc tcggcttgct tgatgagttc ggtcagttct
    18961 cgatgattca tcttattgat atcgatcgcc atggcttatc cctcttggaa gctgttggta
    19021 ataatggata ttggctccga ccttccaaga ttatcaatgc cgtcgtggat tatcaagtcg
    19081 ccgattgttt caatgatgaa aacggtttca gtttttgtct gtcgctacgt taggccccca
    19141 tcatccaacc gaacctatat cgcctgaaac catttctcgt agattgggca caatccccct
    19201 catcacctac gccaacgcgg aaatcgcagg ccgactcacc ccctccacca ccccttcaag
    19261 ccactggctc cccgccatga cctgctcctc cgtcaaactt cgaacccacg caatatgatc
    19321 ctccaccgca tccgccgcca aaggcagggg acaaggcccc ggatccaccg cggatatcac
    19381 cggcaataca cgccctcgtt cctccaaaaa acgcccaagc tgcttgcatc gctcctcgat
    19441 acagccatcg agaagctcga gcgccgcctc gaaaaccgcc ttgcgatgcg gcggacattg
    19501 gaacagacgt accttgtcat accatacccg aagcgcatgt aagtggccga tatagcggat
    19561 atttttctcc gcccggggca tgaccgcgtc caatcgccca aactccaccg ctcggtcgat
    19621 cggtcgggtt tgctgcaaca ccagatgccc ttcggcgatt ccatggcgaa ctacctgtcc
    19681 cgctcctgcc acagccccaa atccaacctc cgccggtccc accaaaccgc ccgtgccccc
    19741 aagaaaaatg cgtcgctccc gcaaaaatac tccccgaggc acgctgccta tcaacgaagg
    19801 cgtcgcctta tcaccatgct tcccccaagg cgtgaagttg aaatgaatgt acccacttcc
    19861 cacttccgca tgatcgctgc gtgacgtgcc ccccgtcatc aaacaatcac aaaaattgat
    19921 caaactcccc agcgtcacga aactcaataa caacgtctgc ttgagcccaa ccgcatgcgc
    19981 cgtcgatgcc tcctcctcca atagcgttcc cgctcgtaca tgcgcattcg ctccaaggga
    20041 ggcctttttc agcaacaccg cgccgtgaac atagccgctc gctacccgcg cctcctcgtc
    20101 catgaccgtg tccatcaacg tcgccggccc ctccgtcccc acctccgctc tcgctccaag
    20161 cctcgttttc gaaccgtgga gccgtacccc aggatgcaac accaccccct ccgcaaccct
    20221 ctccaagggc acatcctcgc caatatatgt ctgccgcgga tcgatgatcc gcacgcccga
    20281 cttctccagc gcttccaacc gctgttcgag tgttcttgtt ttcacgcccc ataatatatc
    20341 acgaatcgtc atcgccaaaa gggcttccga tggctcggtg atatttgtgt aaactgctga
    20401 attgacagga ttattttatc catcggcaga gggaagagcc cccttttccc cccccgtggc
    20461 cacctgcctt tcgccgaaat cacatcacga tataagaccc ggaggctctt gcatacccgg
    20521 gccagccatg ttcacggctt ccgttctccg acgcggcaac cggctgggat ccttatataa
    20581 aaacgcgagc agcttctccc ggatcttgca ccgcaagtcg aataaatccc cggcattcct
    20641 cgcgcttacc gtgatcctga tcaccgccgc atgctccacc acatccgtaa cgagaaccgc
    20701 tttcgcctgc ccatcgaata acggttcatt ctccaaaagc ctgtccaact ccgcccgaac
    20761 cgcatccaca tccaccccaa aatccgcatg caaaaaaacc gtcccccgaa gatccggcga
    20821 taccttcgtc caattctcga acggattgtc gagaaaatag gtgaccggca ccaccaagcg
    20881 acgattatcc cataatcgta ccaccacgtg ggtgctgcca atactctcga tctgaccgta
    20941 ttctttctcc acgatcaccg tgtcgccaat gcgaacgggt tgagaaaagg aaatcaatat
    21001 ccccgcgaat aaattcgaaa atgttttttg cgccgcaaat ccaaggatcg ctcccgcgat
    21061 tcccgccgac ccaagaagac tcaaccccac cgtccgtacc gtcggaaatt gcatcaacgc
    21121 caacgccacc ccaaccaata ccaccaacac ctgcgctatc cgtcgtagcg cgtccagacg
    21181 cgtcatgatg gaacgcaccc gcgccgcatc ctcgtcccgc gcatgacgaa gctgtatcgt
    21241 ctgcaccgat atccgaatga tccgcagcgc actgaccatc agcaccaaaa tgatccccgt
    21301 gatcaacacg cggctgatct gtatcgacgc cgtcgccgat aaccgaaggg tagggaaagt
    21361 ccccaacacc accaacaaca tcaccagcca atgcaccaaa cccttggtca aatccgacgc
    21421 catcgcttca tgcgcgattt tggcccgccg tgccgtgcga cgaatcaacc acacgatcgg
    21481 acgctccaac agcattcccg cgacccagcc ccccactgcc aatag
//
LOCUS       DAOSOR010000026        21451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_2457_length_21451_cov_4.254440, whole genome shotgun sequence.
ACCESSION   DAOSOR010000026 DAOSOR010000000
VERSION     DAOSOR010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 21451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21451
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2457_length_21451_cov_4.254440"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PK710_02550"
     CDS             complement(<1..301)
                     /locus_tag="PK710_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08617.1"
                     /translation="MALLFLRRSHDPDSVVGPLTPDQVWEASPSEDDCLSLRESGPFV
                     PVGVGLNYDDRLREVMLEAFPNPRAYRYARKRLALYLCLVPILLWTLMDGQMLFAG"
     gene            367..555
                     /locus_tag="PK710_02555"
     CDS             367..555
                     /locus_tag="PK710_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08618.1"
                     /translation="MNASWTEWLVVIGLWALVIVVPRWMRPQAKPSRVDSGIRVTFLP
                     DEDRPTPRANGSDLEKTD"
     gene            complement(782..1303)
                     /locus_tag="PK710_02560"
     CDS             complement(782..1303)
                     /locus_tag="PK710_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT08619.1"
                     /translation="MSITIRPATPDDVEFILQLIRDLATFEKAPDAVKATVEDLLRDG
                     FGPHPRFESLIAQLDDRPAGFALYFYNYSTWEGTTGIHLEDIFVLPWARGHGVGRALI
                     GAVAMRAKERRCPRLDLSVLHWNPAREVYRKLGFVPLSEWVSYRLEGEALSQLAARTA
                     EGACVSERVEPDE"
     gene            complement(1360..3153)
                     /locus_tag="PK710_02565"
     CDS             complement(1360..3153)
                     /locus_tag="PK710_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08620.1"
                     /translation="MTWSFRLSCGCAFILAACSSQPSAEPSDSPSLDASTEDGAPLVS
                     YDPVCSADGWCWASPLPQGNGLRSVWGASNSDLWAVGDAGLVFRLQQGRWTPIRTDSK
                     GDLRALWGSGPNNIWAGGEELIHWDGQSWTKTSGPSEILVLEGSAANDIWLTTAGANV
                     YRFDGTAWNETFPPVAGVYRDVWVGAANDVWLVGDAGACVHWDGSTWQSRPEVGTRNF
                     TGVWGTQGKGVWAISHGAGSNDRTRIHHWDGGWKAVFDAEPHEHPQAIGGTSEQDIWV
                     VGANGLALHWDGATWRRHGTGTTRKLEALWAMGDECWAVGAVGAIIHWHASSWNKVTE
                     GGETWMNDLWVAGANDVWTVGQEGAMAHFDGAGFVTTDTGDRTRLASVWASGPEDIWA
                     VGASGVIVRGIDGKWSRVSAGTSRDLLAVTGCGAEDVWVGGVQGTLLHWDGKAWRSHE
                     GAAQGNVQSLWCGGKDDVWAGGQAGLLIHWDGNDWNSATLSISADIINLWASGSKNVW
                     AVTASGLLHWDGQVWEPVEIGEGGLSSLWGSSDRDIWAAGPAGRLLHFDGESWLRSDP
                     GTRSDILSVGGSSDGDVWILTSFGGILRKHR"
     gene            3364..5301
                     /locus_tag="PK710_02570"
     CDS             3364..5301
                     /locus_tag="PK710_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08621.1"
                     /translation="MPRSQPNPRKTTLPEQAVRALFLLEHHVRNAFSMRQLPMFEDAD
                     KIWLSKTRLGLVTVVLRSAIVLHAEAQGLLPLGDSAYDRGYAWTPLAQQRTAKGAWRR
                     FLRLSNALSGKTHAILKPWQSEVFSGAWLGPLGPPAEPHSPEALSDAAIHAVFRALLP
                     TSREENFEIDHVVALYERLMEFELVRCRGRTIRWGTTHALVDLDELSKVPPTARGEYL
                     SLRGIKPSQALLQRLMGAADVDDMLAVIPARTRNTLLPAGSVALQANLVARDTPAPHT
                     PRELIHKTVRATLEPLVEAAQGASDRLLALRICDPSMGTGQFLTEACRQLAYAVVAAD
                     STRQNDATALAQARWQVASRCLYGVDGDPIALELARLSLRLIGMKPDGTMPEINRHLR
                     HGDALVGIGPWHGSPARRRPDPRLYSLHWASTAMARPTTHGAPRKTGRNRSPSRVESL
                     RLLADQLIREILMNGHHKARKRLTIDEPMMLREASITENRAAYFHWPLEFPEIFESPE
                     PGFDAFVGKPPWVSCSGVAAHPLANDLKRYFTSLSSAFQRNRPLHGLFVHRSASLLKP
                     GGRLGLILPPSVASLGGLAPLRQVHDALCRVDMPLPHYGGRTSQGIVQPTMALLSTRR
                     QDGESIGEPQVWPLHRDKLDE"
     gene            complement(5419..5844)
                     /locus_tag="PK710_02575"
     CDS             complement(5419..5844)
                     /locus_tag="PK710_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT08622.1"
                     /translation="MKDKPPVVYLVDDDVDYCEVNRTVLESAGYRAVCFPGPKEALLE
                     AERNPPDLIITDMMMGSLDDGLRLSRSLRDHASLCHIPVLLVTGLVRELGLDIVPRDS
                     QDLEALNASAFLEKPTSPSRLLQEVRRLLAEASDPAAPR"
     gene            complement(5841..7679)
                     /locus_tag="PK710_02580"
     CDS             complement(5841..7679)
                     /locus_tag="PK710_02580"
                     /inference="COORDINATES: protein motif:HMM:NF040763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOT08623.1"
                     /translation="MPTLTINNITVQAPEGATVLEAARAAGIHIPTLCHLPGVHAPGA
                     CRVCLVEVEGARTLMASCVTPANEGMRVHTNSLRARNGRRDVVELLLSEHEGDCTTCS
                     RSEDCELRAIAREAGVKDIQYRGERRTTRLDYSTKGLVRDTGKCILCRRCVAVCQDVE
                     SVGAIAPQERGFGTYIGPAFHAPLSEVTCVQCGQCSAVCPVGAIGERDQIDEVWRALD
                     NPSLHVVVQTAPAIRAALGECFDLPPGTLVTGKMVSALRRLGFEAVFDTDFSADLTIM
                     EEGHELLARLQGALAGGANKSPLPMFTSCCPAWIQFAEHFAPDMLDNLSTCKSPQQML
                     GALAKSYYAEKIGKKPEEIFVVSIMPCTAKKFECDREEMRDSGAKDVDVVLTTRELGR
                     MIREAGIDFGSLADEEMDAPLGISTGAADIFANSGGVMEAALRTAYEVVTGRPLPPVQ
                     LEPVGKMAGGGVLQASLQVSDALPAWSFLEGQTLNVAVVHGLAHARALIEDIRAGRQS
                     PHFVEVMTCPGGCIGGGGQPRMTTDEVRRARIQAICQEDASKPLRKSHTNPGVTQLYE
                     EYLGTPLGERSHHLLHTHYHPRCFVPKAPAKEKVAPRPHSEVRPEP"
     gene            complement(7693..10743)
                     /locus_tag="PK710_02585"
     CDS             complement(7693..10743)
                     /locus_tag="PK710_02585"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOT08624.1"
                     /translation="MANSIRTHILVCTGGGCIACGALQVSAALRQSLRAHELQDEVKV
                     VETGCLGPCSVGPVSVVYPDGVFYENIKPEDAEEIVTEHLLKGRPVERLSRSNLLAEQ
                     EKPEQKAGFFDKQVKIVLRNCGIIDPRKIEEYIARDGYEALARVLTEMTPDQVIDEMK
                     KSGIRGRGGAGFPSGLKWAIAKKSTGSPKYVLCNADEGDPGAFMDRSVLEGDPHSVIE
                     GMAIAARAIGANRGIVYVRAEYPLAVERLRLAIEQAHDLGLLGNDILGTGFEFDLEIR
                     MGAGAFVCGEETALITSIEGNRGEPRPRPPYPADRGLWGKPSLLNNVETYSNVPMIML
                     KGADWFASLGSDKSKGTKVFALAGAVHNSGLVEVPIGTKLGEIIYDIGGGIPAGKAFK
                     AAQVGGPSGGCIPRQHLEVPVDYESLQELGAIMGSGGLVVMDEDSCMVDVARFFLEFV
                     QEESCGKCTPCRVGTKRMLEIVTRICEGKGEESDIERLEKLGRTIQQTALCGLGQSAP
                     NPVLSTLRHFRDEYVAHIRDKRCPAGVCADLVRSPCQNACPAGVNVPGYVSLIGEGRT
                     AEALHLHRENNPFAAVCARVCFHPCESKCRRATLDEAVSIRGVKRFMADAEQGLQLPS
                     TRIDPACAKRKVAIIGAGPAGLSCGYFLARLGYRPDIFEAAPRPGGLLLQGIPAYRLP
                     REVIDAEVAMIERLGVTIHTGQRLGRDITLSGLREAGYESVFLGIGAPLGTRLALPGE
                     DAKGVVDAMQFLRDFNLTGTAPVGKNVVIIGGGNSAIDAARTAIRLGAAQVTVVYRRT
                     RDQMPAFPHEVQEAEREGVRIRFLLSPTEVLVKDGAVAGLRCVPMELQGFDRAGRRRA
                     VPSQQPTVDVPADMLITAIGQQLDLSTFGSDVMPKTSRDGYVSVDPRTGQTDILWLFA
                     GGDAASGPSSVVEAVRDGERAAVAIDRMLSGKSRAFWREHRSVDTFFDPDADPSTAPR
                     THQRLLAIEKRLGRFDEVELCWTETDARREAARCLRCDYRETCG"
     gene            complement(10762..11133)
                     /locus_tag="PK710_02590"
     CDS             complement(10762..11133)
                     /locus_tag="PK710_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOT08625.1"
                     /translation="MDKRIDSREALSALRARVRQQLKPREGAEALRITVHMGTCGIAA
                     GARDIIHHFAEQLDRENLHEVALTRAGCAGLCDQEPMITLRDASGTEFRYGRLDQRKI
                     EEIVQGHVLQGVPVRKYLITA"
     gene            complement(11108..11686)
                     /locus_tag="PK710_02595"
     CDS             complement(11108..11686)
                     /locus_tag="PK710_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOT08626.1"
                     /translation="MDGSAALEDDEMDMVQVSCNCGIEVSEEELLERLDATIAEYRGK
                     PGALIPVLQIAQGIFGHLPEVAMRRIAEGLDKPYSEVSGVVTFYSYFSTVPRGRHVIR
                     VCLGTACYVRGGKAVLEAIRKTAHVEVGGTTEDRRFTLEVARCFGACGLAPAIMIDDE
                     VYHRVKPSRVQGVLESHASKFGGQHGQANRQP"
     gene            complement(11701..12246)
                     /locus_tag="PK710_02600"
     CDS             complement(11701..12246)
                     /locus_tag="PK710_02600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT08627.1"
                     /translation="MREIALHVLDLMENSVAAGAQRVLVRVVQNRATDQLEIAVEDDG
                     CGLPDTVQDAAHPFFTTKPGKRTGLGLSLFREAAERAGGDLHLCASPWGGTAVRVRMR
                     LLHIDRMPLGDLAATVGAMACTAPEVDFSCEVEVDGKKETVSTQQLRAATGQGPGQNL
                     LVMRQMTNQIRKGLEGLGALP"
     gene            complement(12239..12898)
                     /locus_tag="PK710_02605"
     CDS             complement(12239..12898)
                     /locus_tag="PK710_02605"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HOT08628.1"
                     /translation="MKSLALDLHLHSTLSPCADPSMDPRAVVRRAKDCGLSLIAFCDH
                     DRIGAAQEPFSVDGLAVLQGVEITTLEKTHLLGIFPHWEAAVALARELGLPHGKNTER
                     AHRKGNYPTLPEAVRVIHNVGGLAIAAHVDRPSSGLLGKVGCIPNVGLDAIELSASGL
                     YRIDTLHLAQYGLPVLVGSDSHDLSEIGSVYCTVSLRSACFEELAQALREASDWRCHS
                     A"
     gene            complement(12895..13257)
                     /locus_tag="PK710_02610"
     CDS             complement(12895..13257)
                     /locus_tag="PK710_02610"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOT08629.1"
                     /translation="MNSSLTLRTIATQLGLEDLTAENQAIDPPVTVGYASDLLSDVLA
                     HAPAGAVLVTTQAHLNVLAVASHVGLAAVIFTGGVQPSPDVLLRAVEEGIPAFRTLCT
                     SFDVAGRLYALGLRGGTR"
     gene            complement(13254..14813)
                     /locus_tag="PK710_02615"
     CDS             complement(13254..14813)
                     /locus_tag="PK710_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT08630.1"
                     /translation="MTVFHHTIVGGDFERAGHATDALKDLLKTVGVDPESTRRAAIAA
                     YEAELNVIIHARRGILRATIGDDRLDIEIEDEGPGVADVALALQPGFSTASQQARSLG
                     FGAGLGLPNIKRNSDTFSLESTVGVGCRLRFGLSFSCVEPLPPAPNALRFVSERCRLC
                     LRCLPVCPTQALRVRGTGPSLLAHRCVDCNACIAHCIHGAIQIDNDSPCPDAGSLDVL
                     VLPLAFLTDFGPNVSYRLIREIILQLGFSRLALLEQWEAAQQNVIDELLAQNNHPLPI
                     IDVACPVVTRLIETRFPSLLPHLAPLLSPLEAAAAQLQGQKAAFVVACPAQQTSLRQA
                     GVPSQRILTPRALRQAIDQRLRAKRPTDQDSDCPPIRAAVERNELMASGLASTLRVLE
                     TIEGERLEFTQRVMLRACVDGCFGSPLFRTPSALALHRSRRMTINDDATYCVARRQSP
                     YQPRPALRLDSDMQASVKKLHRMAEVLRSLPQADCGRCGAPSCRSHAEDYALGRIRSL
                     QCKHPSARSQS"
     gene            15257..16753
                     /locus_tag="PK710_02620"
     CDS             15257..16753
                     /locus_tag="PK710_02620"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT08631.1"
                     /translation="MATVRTWKCEVCGYVHEGPEPPETCPVCGVGKEMFSPLEFAPEP
                     VAPSPPPKAWRCTVCGHIHEGDHPPETCPVCGVDAGLFEPIAPEKPVTSAPQGPRIVI
                     VGAGIAGVIAAARARETSSDVQITLVAKEQGLPYYRLNLTRFLANEVTENDLPLYPET
                     WFEKNHIELIHGDCTAIDRQSRQVLLRDDRRLAYDRLILAIGSHPFVPPIAGVTRQGV
                     HSFRTLSDAKALAALAGPARRCVCIGGGLLGLEAAGALRKRGCEVTVLEGFGWLLPRQ
                     LAQPAGQMLQDHLQKLGVHVITNARIKQIDGDEAVRAVQLEDGTELPADLVVLSTGVR
                     PNSFLARQCGLEVKHGVVVNDRMETSDATIFAVGDLAEHRGQVPGLWPFSYAQGAVAG
                     VNAAGGSAEFHGMPRSNQLKVLDVSMVSIGEFQPADASFRVFELQEPGKYLRLVCRDG
                     ALVGANLYGDAALSSLVREAVESGRPLAEQLELLKHIPALRTSCERGA"
     gene            16799..17374
                     /locus_tag="PK710_02625"
     CDS             16799..17374
                     /locus_tag="PK710_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOT08632.1"
                     /translation="MPSLKGTRTEKNLLAAFAGESQARNRYTYAAGIARKEGYRQIEA
                     LFLETAENEREHAKVFFKHLEGGMVEITATYPAGVLGTTLENLEAAAAGENEEWTALY
                     PAFAEIAKEEGFPAIAASFTMIARVEKEHEERFRKLAANVKDGKVFKKEKPVRWKCRN
                     CGYVHEGTEPPGKCPACAHPQEHFEIKEDNY"
     gene            complement(17371..18630)
                     /locus_tag="PK710_02630"
     CDS             complement(17371..18630)
                     /locus_tag="PK710_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT08633.1"
                     /translation="MIKALETEGAMNVRRLRKPTIFGSYFRWALVATLIVGLPGCSGS
                     SMGESKAVSSRVESAPEPEPERRPAAILAKWFENLPCPLPPPLPFVEGSSTLAVLPDT
                     QVYTDRYPETFERQVRWIVEQKQTYDIRFVLHLGDMTERSSAREWQTAEKTMGLLDGV
                     IPYAIATGNHDYEDKGWANTRRSPLSTHFPVAKMRKVGALGGTYNAGVLDNSYHLFSA
                     GNRDWIALMLEWGPRRGVVDWANKVLQTYADRTAIVVTHAYLFHDNSRYDRIRDDQPW
                     NPHHYPTAKLPGGVSDGEELWNDLISHHGNIAMVLCGHVLGDGAGYLASKGDVGNTVH
                     QMLSNFQMMPGGGRGYMRLLELLPDGKTVQVKTYSPMLDDFMTDDQHQFVIELDVRLG
                     RPCKNNQPCDPYADRKKTSGDGANEGG"
     gene            18723..19268
                     /locus_tag="PK710_02635"
     CDS             18723..19268
                     /locus_tag="PK710_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08634.1"
                     /translation="MQKPVSMTVLGVLNLVFALIGLLSTIASTLVMLSQATTNPVYVI
                     MAANPTYRFYVYASLLVGVALISLLAAGGIGLLLSKAWGRICTILYAIGTILTGLISV
                     VVNYVFLIAPLVERSAHTKTPEEIGALGGAIGGTLGGCFGLLYPVILLIFMYRKNVVQ
                     FFNPSETTPSSDAPAWEYDRR"
     gene            complement(19207..19938)
                     /locus_tag="PK710_02640"
     CDS             complement(19207..19938)
                     /locus_tag="PK710_02640"
                     /inference="COORDINATES: protein motif:HMM:NF036823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOT08635.1"
                     /translation="MIVSIPIWIAILEGEVQSQSGLHPRERQSLSPRAIPKRKDDFLR
                     GRTVAKALVHRRTGLDPRDVAIVADARGVPWIEHDRHGTMPFSLSISHTKGVASAALA
                     PLPWLVGVDVEHPIEQPAAIATDYFEPKEQAYCKGADGLERRWRAAEIWTLKEAGLKA
                     LGTGLTIPASAIVVQDIEDSASFQGWRAATIAFGSSAGHQDHVAHAWVQRCSSVMLGL
                     VVLVDPTDDRIPTPERPMRVSFPRD"
     gene            complement(19959..>21451)
                     /locus_tag="PK710_02645"
     CDS             complement(19959..>21451)
                     /locus_tag="PK710_02645"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT08636.1"
                     /translation="DPQQVEAIVKDILERHGRLDLIVHAAGLERSRGLDTKEPAEFNL
                     VFDVKVMGMFNLLAATRNVDVGAIVSFSSVAGRFGNAGQTDYSSANDFLCKVTSAMTW
                     LRPKTRGIVIDWTAWGSIGMATRGSIPEIMKRAGIELLDPDLAVPVVRQQLQGGQQGE
                     VVISGALGILTEPRDADGGAKSSEPMPTSEKGPFAFKVLRSDPYEGVVMTTMLEPEQP
                     YLDHHRIDGVAVMPGVMGLELFAQAVAVLMPGISVRSLSDVRFAAPLKCYRNEPREAI
                     VTVRLLRGADGDRAVCSLHSIQSIKGSEKAAEPKLHFSAILELASESAEGATVEALSP
                     KAEGGNVERQDLYRAYFHGPAFQVLEGTRVGADGATLLGKMMHPLPDLTREPRSMLSA
                     PRLLELCFQTAGVIEIGSTRKLGLPSKIEQVRVFAGAEQSGAWTARVVMGSRSDGLRF
                     DAVVTSDSGKILLEVVGYRTSALPEQMDPQVWAPLGGGLLGFEPAP"
BASE COUNT         4504 a         6713 c         5954 g         4280 t
ORIGIN      
        1 ttcccgcaaa gagcatctga ccgtccatca atgtccacag caggatcgga accagacaca
       61 agtacagcgc caatcgcttg cgggcataac gataggcccg cgggttggga aatgcctcca
      121 gcatcacctc ccgcaaccga tcatcgtagt tcagacccac ccccaccgga acgaaagggc
      181 cggattcacg caacgacaag cagtcgtctt ccgatggact tgcctcccat acctggtcgg
      241 gagtcaaagg gcccacgaca gagtccggat cgtgtgaacg tcgcagaaat aataaggcca
      301 tgaacctcgc ccattgattt ggacttaact acgccaacct catgccgagg tggtaaacaa
      361 ccgtgcgtga acgcttcgtg gaccgaatgg ctggtcgtga ttggcttatg ggcccttgtg
      421 attgtggtac cgcgatggat gcgcccacaa gcgaaacctt ctcgcgtgga ctcggggatt
      481 cgtgtgacgt tcttgcctga tgaagatcgc ccgacaccac gagccaacgg cagcgatctc
      541 gaaaaaacag attgaaccac gacgtcgcca gccgatcacc tgacgcctcg tttctaccca
      601 cgatcgttca gcccttgtca aacacgtctc tggggttagt cgacgatcga ttaggacgaa
      661 cgaccgggta gcggaccggc tccacagcaa ggcatttttg cctcaccctc atcgtgccat
      721 gctggcaaac cgaactggtt tggatggcct cattcggcaa gagtcatcga aagatgcatg
      781 ctcattcgtc tggttcgaca cgctcggaca cacaagcgcc ttcggcggtt cgtgccgcga
      841 gttgcgataa tgcctcgcct tccagacgat acgaaaccca ttcgctcaac ggcacgaagc
      901 caagcttgcg ataaacctcc cgggcgggat tccaatgcaa tacggaaagg tcgagccttg
      961 gacatcgacg ttctttcgca cgcatcgcca cggccccgat cagcgcacgt cccacaccat
     1021 gacctcgagc ccagggcaaa acaaaaatgt cctccagatg gatgccggtc gttccttccc
     1081 atgtggaata gttgtagaaa tacagtgcaa atccggcagg ccgatcgtcg agttgcgcaa
     1141 tcaacgactc gaaccgtggg tgcggaccaa aaccatcgcg aagcaaatct tcgacagtcg
     1201 ccttcaccgc atccggagct ttttcaaagg ttgccagatc gcgaatgagt tgaagaatga
     1261 actccacatc gtctggagtc gctggccgaa tcgtgatgct catcaggcaa cggatcgtag
     1321 gcccacggtt cgaggatggc tatgaaaaca cggtgcccct cagcggtgct tgcgcaaaat
     1381 ccccccaaaa gatgtcagta tccacacatc accatcgctg ctgcccccca ccgaaaggat
     1441 gtcgcttcgc gtaccggggt ctgaccgcaa ccaggactcc ccgtcgaagt gcaataatcg
     1501 acccgcgggt ccagccgccc atatatctcg atccgagctt ccccaaaggc tcgacaaccc
     1561 gccctcacca atctcgactg gctcccaaac ctgtccatcc caatgcaata accccgacgc
     1621 ggtcacggcc catacgtttt tcgaaccgct tgcccaaaga ttgatgatat ccgccgaaat
     1681 cgataacgtg gcggaattcc aatcgtttcc atcccaatgg atcaacagtc cggcctgccc
     1741 ccctgcccat acgtcatctt ttccgccaca ccataaagat tgtacattgc cttgcgccgc
     1801 cccttcgtgg cttcgccatg cctttccatc ccaatgcagg agcgttcctt gtactcctcc
     1861 cacccataca tcttcggctc cacaccccgt cacggccaac aaatcgcgag aggttcccgc
     1921 ggaaaccctg gaccatttcc catcgatccc gcgaacaatc accccagagg ccccaacagc
     1981 ccaaatatcc tcgggccccg acgcccatac gctcgccaaa cgagttcggt ctcccgtgtc
     2041 ggtagtcaca aaaccggccc catcgaagtg cgccatcgct ccttcttgcc ccacggtcca
     2101 aacatcgttg gcacccgcga cccaaagatc gttcatccac gtttcgcccc cctcggtcac
     2161 tttattccat gaacttgcgt gccaatggat gatggctccc accgcgccaa ccgcccagca
     2221 ttcgtctccc atggcccaaa gcgcctcgag ctttcgtgtc gtgcccgtac catgccgacg
     2281 ccatgtggcc ccatcccagt ggagcgccag cccgttggct cccacaaccc atatatcttg
     2341 ctcactcgtt cccccgatgg cctggggatg ctcatgcggt tccgcgtcga aaacagcttt
     2401 ccagccgccg tcccaatgat ggattcgggt ccggtcgttc gagcccgcac catgactgat
     2461 ggcccacacc ccttttcctt gcgtacccca cacgccagta aagttccgag ttcctacctc
     2521 cggcctgctt tgccacgtcg acccatccca atgcacacaa gctcccgcat ctcccacgag
     2581 ccagacatcg ttcgcagccc caacccacac gtcccggtac acgcccgcca ccggcggaaa
     2641 cgtttcgttc caagcagttc cgtcgaaccg atacacgttc gcccccgcgg tggtgagcca
     2701 tatatcgttg gccgccgatc cctcgagcac aagaatctcc gaaggcccag acgttttcgt
     2761 ccaagattga ccgtcccaat gaatcaattc ctcgccacct gcccagatat tgttgggtcc
     2821 agaaccccaa agggcgcgca gatccccttt cgaatcggtt cgtattggcg tccaccgacc
     2881 ttgctgtaac cgaaacacaa gccccgcatc ccccacagcc caaaggtccg aattcgacgc
     2941 tccccacacg gatcgcaaac cattgccttg cggcagaggc gaggcccaac accaaccatc
     3001 cgcactacac accggatcgt aggaaaccaa cggcgcaccg tcctccgtgc tcgcatcgag
     3061 cgacggagag tccgatggct ccgccgaggg ttgagaacta caggcagcta aaatgaaggc
     3121 acaaccacaa gaaagccgaa aagaccaagt catcaactcc gaagcctagc aaaagcaatc
     3181 gaaaaatcgt cgcattttag tcgctaccca tacccatcca ctcccgtcgc acaccaaacc
     3241 cgaatccaag cttggcgacc cgcggggaaa tacggcccac tgctgcattt ttcacgttct
     3301 tctccctggc tcttcgcccc aaaaccaggc tatgtgtgcg cgcaccccat cgcctttgcc
     3361 gtcatgcctc gctctcaacc caatcctcgg aagacgactc tccccgaaca agctgtccgt
     3421 gcccttttct tgctggaaca ccatgtgcgc aacgcttttt cgatgcgcca actgcccatg
     3481 ttcgaggatg ccgacaagat atggctctcg aaaacaaggc tgggcctcgt taccgtcgtg
     3541 cttcgatccg ccatcgtgct tcatgcggaa gcgcagggtc tgcttccctt gggcgatagc
     3601 gcgtatgatc gcggctatgc ctggacaccc ctggcccaac agcgtacggc caaaggggca
     3661 tggcgaaggt ttttgcgcct ttccaacgcc ttgagcggga aaacacacgc aatattgaag
     3721 ccttggcaat cggaggtctt ctccggggca tggctcggac ctctgggacc gcccgcggaa
     3781 cctcattccc ccgaagcctt gtccgatgcg gcaatccacg ctgtttttcg tgcgctccta
     3841 cccaccagcc gagaagaaaa cttcgaaatc gaccacgtcg ttgcattgta cgaacggctg
     3901 atggaattcg aactcgttcg ctgccgaggc cgaacgattc ggtgggggac gactcatgcg
     3961 ttggtcgatc tcgacgaact atccaaagtc ccacccactg cgagaggcga gtacctcagc
     4021 ctgcgtggaa tcaaaccgtc ccaggctctg ttgcagcgat tgatgggcgc cgccgatgtc
     4081 gacgacatgc tcgcggtcat tccagcccgc acacgaaata ccttgcttcc tgccggcagt
     4141 gtggctcttc aagccaatct cgtcgcacgc gacacgccag cgccgcacac ccccagagaa
     4201 ttgattcata agacagttcg ggccacgctc gagccattgg tcgaagcggc ccagggagcc
     4261 tccgatcgct tgctcgcact gcgaatctgc gatccgtcca tgggtacggg ccaatttttg
     4321 acagaagcat gccggcaact cgcctatgcc gtcgtggccg cggattcgac ccgacaaaac
     4381 gatgccacgg cgcttgcgca ggctcggtgg caggtcgcat cccgttgcct ttatggtgtg
     4441 gatggggatc ccattgcgct cgaactggcg cgtttgagcc ttcgactcat tgggatgaaa
     4501 ccggacggta caatgcccga aatcaatcgc catcttcgtc atggtgatgc cctcgtggga
     4561 attgggccct ggcatgggtc ccccgcgcgc agaagacccg atccgcgatt gtattcattg
     4621 cattgggctt ccacggccat ggcgcgtccg accacgcatg gggcaccgag aaagaccgga
     4681 cgaaatcgct caccgtctcg tgtcgagtcc cttcggctgc tggcggacca gctcatccgg
     4741 gagatcctga tgaacggtca ccacaaagcg agaaaaaggt tgaccatcga tgaaccgatg
     4801 atgctgcggg aagcgagcat caccgagaac cgcgcagcct acttccactg gccgttggaa
     4861 tttcccgaaa tattcgagtc gccagaaccc ggtttcgacg ctttcgtggg taaacctcct
     4921 tgggtaagtt gctcaggcgt cgcggctcac ccccttgcca acgatctcaa gcggtacttc
     4981 acctctctga gttcggcgtt tcaacgaaat cgcccgttgc acggtctgtt cgttcatcgg
     5041 agcgcatcgc tcctgaaacc cggcggacga ctcggcctga tccttcctcc gtccgtggca
     5101 agtctcggcg gccttgcgcc cctgcgtcag gtgcatgatg ccctttgtcg tgtagacatg
     5161 cctcttcccc actatggtgg ccgcacttcg cagggcatcg ttcaacccac gatggccctt
     5221 ctttccacga gacggcaaga cggcgaaagc atcggggagc cgcaagtatg gccgttgcat
     5281 cgcgataaac tcgacgagta gcgagtatcg ttcgaggcat ttcgagaatc gtgaaagcca
     5341 caatttcggt attggccgaa acaacgacgt tcacgagagg cggacgtttt atctacataa
     5401 aacgttgact cttctgagct atcggggggc agccgggtcg gaagcctcgg ccagcaagcg
     5461 tcgcacttct tgcagcagtc gggaaggaga ggtgggtttt tcgagaaagg cgctggcatt
     5521 caatgcctcg agatcctggg aatcccgagg cacgatatcc aagccaagct cccggaccaa
     5581 tcccgtgacc agcagcacgg ggatatgaca taaactcgcg tgatctcgaa gacttcggga
     5641 aagtcggagc ccatcgtcga gcgaccccat catcatatcg gtaatgatga ggtccggggg
     5701 attgcgctcc gcttccaaca acgcttcttt gggacctggg aagcaaacgg cacggtaacc
     5761 ggcggactcg agcacggtgc ggttgacttc gcagtagtcc acatcatcat cgacgagata
     5821 taccacggga ggcttgtcct tcacggctct ggtcgtacct ccgaatgcgg tctgggtgcc
     5881 accttttcct tggccggggc tttgggcacg aagcatcgcg ggtggtaatg ggtatgtagc
     5941 aagtgatggg agcgttcacc caaaggagtt ccgaggtact cttcataaag ttgggtgaca
     6001 cccgggttgg tatgtgattt tcgcaagggt ttgcttgcat cttcctggca aatcgcctgg
     6061 atgcgggccc tgcgcacttc gtcggtggtc attcgcggct gtcctccgcc tccgatgcag
     6121 ccaccggggc acgtcatcac ctccacgaaa tgggggcttt gccgtccggc acggatgtct
     6181 tcgatcaaag cacgagcgtg tgccaagcca tgcaccacgg ccacgttgag tgtttgcccc
     6241 tcgagaaaag accatgccgg cagggcatcg ctgacttgca acgacgcttg aagcacgccg
     6301 cctccggcca tttttcccac gggttcgagt tgaacgggtg gcaggggtct tccggtcacg
     6361 acttcataag ccgtgcgcaa agccgcttcc atgacgccgc cggagttggc aaaaatgtcc
     6421 gcggctcccg tagaaatccc caacggcgcg tccatttctt cgtcggcgag agacccgaaa
     6481 tcgattcccg cttcccggat cattctgccc aattcgcgcg tggtcagcac cacatcgacg
     6541 tctttggcac cgctgtcacg catctcttcc cgatcgcatt cgaatttttt cgcggtgcag
     6601 ggcatgatgg ataccacgaa gatttcttcg ggttttttgc caattttctc cgcgtagtag
     6661 gatttcgcca gggcgccaag catctgctgc ggggacttgc aggtagacaa attgtccagc
     6721 atgtccggcg caaagtgctc cgcaaactga atccacgcgg gacagcaact cgtaaacatg
     6781 ggaagcggac ttttgttggc tccccctgcc agggcccctt gcaaccgagc cagcagttca
     6841 tgcccttctt ccatgatggt aagatccgcg gaaaagtccg tatcgaagac agcctcgaaa
     6901 ccaagcctgc gcagggccga caccatcttt cccgtcacca acgtgcccgg aggcaagtcg
     6961 aaacattcgc caagcgccgc acggatcgcg ggtgccgtct gcaccaccac gtgcagcgag
     7021 ggattgtcca gcgcgcgcca gacttcatcg atctggtccc gctcaccaat ggctcccacc
     7081 ggacagaccg ccgagcattg tccacattgc acacacgtga cctcggacaa gggagcgtgg
     7141 aacgccggac caatatacgt cccaaatccg cgctcttgcg gcgcaatcgc ccctacgctt
     7201 tccacgtctt ggcaaaccgc cacgcacctt cggcatagaa tgcacttgcc cgtatcgcgc
     7261 accaaacctt tggtcgaata gtccagacga gtcgtacgac gctctccgcg gtattgaatg
     7321 tcttttacac ccgcttcccg agcgatggcc cgcaattcac aatcctcgct ccgagaacag
     7381 gtggtgcaat ctccctcatg ctcactgagc aaaagctcga cgacgtctcg tcgcccattg
     7441 cgagcccgta aagagttggt gtgaacgcgc attccctcgt tggcgggggt aacacacgac
     7501 gccatcagcg ttcgtgctcc ttccacctcc acgaggcaga cgcgacaagc gcccggggca
     7561 tgcaccccag gcagatggca aagcgtgggg atatgaattc ccgccgcccg tgccgcttca
     7621 agaacagtgg ccccttcggg agcctgaacc gtgatattat tgatcgtcag cgtcggcatg
     7681 acgtgattct cctcatccac aagtctctcg gtagtcgcat cgcagacatc gagcggcttc
     7741 tcgccgagca tctgtctcgg tccagcacaa ctcgacctcg tcgaagcggc ctaggcgttt
     7801 ttcaatcgcc agcaaacgct ggtgcgtccg cggcgccgtc gaaggatccg cgtccggatc
     7861 gaagaacgta tccacggagc gatgctcccg ccagaaagca cggcttttgc cgctcagcat
     7921 ccggtcgatg gccacggccg ctcgctcccc atcccgaacg gcttccacca cggacgacgg
     7981 tccagaagcg gcgtcacctc ccgcgaatag ccacaaaata tcggtttgac cggtacgcgg
     8041 atccacgctt acatagccat cccgactcgt tttcggcatc acgtcggagc caaacgtaga
     8101 caggtcgagc tgctggccaa tcgcggtaat cagcatgtcg gccggcacat ccaccgtcgg
     8161 ttgttggctg ggaaccgccc ggcgacggcc cgcgcggtcg aagccttgca gttccatggg
     8221 tacgcagcgc aaccccgcca ccgcgccatc ttttacgaga acctccgtgg ggcttagcaa
     8281 gaaacgaatg cgaacgcctt cgcgctccgc ctcctggact tcgtggggga aagctggcat
     8341 ctggtcgcgg gtgcggcgat acactacggt aacctgggca gcgccgagac gaatggcggt
     8401 gcgagcggcg tcgatggcgg agttaccacc gccgatgatg acgacgttct ttcccacggg
     8461 agcggttccg gtcagattga aatcacggag gaattgcatg gcgtcgacca cccctttggc
     8521 atcctctccg ggcaaggcca ggcgggttcc caacggggcg ccgataccga gaaatacgct
     8581 ttcatatcca gcttcccgca agccagacaa ggtaatatcg cgccccaaac gttgccctgt
     8641 gtggatggtg acccccagcc tttcgatcat cgccacctcg gcatcgatca cctcccgggg
     8701 cagacggtac gccgggattc cttggagcaa gagtcctccg ggccgcggcg ccgcttcgaa
     8761 gatgtccggt cgatatccga gacgagcgag gaagtagccg caggacaaac cggcaggtcc
     8821 cgctccaatg atggccacct tacgcttggc gcaggccggg tcgatgcggg tcgatggcaa
     8881 ttgcagccct tgctcggcat cggccatgaa acgtttgact ccacgaatcg ataccgcctc
     8941 gtccaaggta gcgcgacggc atttcgattc gcacggatgg aagcagactc ttgcgcatac
     9001 agcggcgaag gggttgtttt cccggtgaag atgaagcgcc tcggcggtgc gtccctcacc
     9061 gatgagtgac acataaccag gaacgttgac gcctgccggg caagcgtttt ggcaagggct
     9121 tcgaaccaaa tccgcgcaaa cgcccgcggg gcaacgttta tcgcgaatat gcgccacata
     9181 ttcgtcacgg aagtgccgca gcgtggaaag cacgggattg ggagcgctct gccccaggcc
     9241 gcaaagcgct gtttgctgga tcgttcttcc cagcttctcg agcctttcga tgtcggactc
     9301 ctcgcccttt ccctcacaga tgcgcgtcac gatctcgagc atccgtttgg tgcccacacg
     9361 gcagggggtg cacttcccac aggattcttc ttgcacgaat tcaaggaaaa accgagcgac
     9421 atcgaccatg caggagtctt cgtccatgac gacgaggccg ccggaaccca tgatggcgcc
     9481 gagttcttgc aaagactcgt aatccacggg cacttcgaga tgctggcggg ggatgcagcc
     9541 gccggaggga ccaccgacct gagcggcttt gaaggccttg cctgccggga tcccaccgcc
     9601 aatatcataa atgatttcgc caagtttcgt gccgatgggc acttccacca aaccgctgtt
     9661 atgcacggct ccagcgagag caaatacttt ggtgcctttg cttttgtcgc tgccaaggga
     9721 ggcgaaccaa tcggcccctt ttagcatgat catgggaacg ttggagtacg tttccacgtt
     9781 gttgagcagg cttggctttc cccatagtcc gcggtcggcg gggtaaggag gacgaggacg
     9841 gggttctccg cgattgcctt cgatcgaggt gatgagcgcg gtttcttcgc cacagacgaa
     9901 agcgccggct cccatgcgga tctccaggtc gaattcgaag ccggtgccga ggatatcgtt
     9961 gccgagcagc cccaagtcgt gcgcttgttc gatggcaaga cgcaaccttt cgaccgcaag
    10021 aggatattcc gctcgaacgt agacgattcc acgattggcg ccaatcgccc tggcggcgat
    10081 ggccatacct tcgatcacgc tatgggggtc tccctcgagg acgctgcggt ccatgaaagc
    10141 gccgggatcc ccttcatcag cgttacacaa tacgtatttg ggagagccgg tggacttttt
    10201 ggcaatggcc cacttgaggc ccgaggggaa acccgcgccc cctcgaccgc gaatccctga
    10261 ctttttcatc tcatcgatga cctggtcggg ggtcatttcc gtcagaaccc gagccagggc
    10321 ctcatagcca tcgcgcgcga tatattcttc gatttttcga ggatcgatga ttccgcagtt
    10381 gcgcaagacg atcttgactt gtttgtcgaa aaatccagcc ttttgctctg gtttttcttg
    10441 ttccgccagg agattcgagc gactgagccg ctcgacgggg cgtcctttga gcaggtgctc
    10501 ggtgacgatc tcttcggcat cttccggttt gatgttctcg taaaagacgc catcgggata
    10561 gacgacggat acggggccga ccgagcaggg gcccaggcaa ccggtttcca cgactttgac
    10621 ttcgtcttga agttcatgag cacgcagcga ttgccgtaac gcggcactga cctgcagggc
    10681 accacaggca atgcatccgc ctccggtaca gacgaggata tgggtacgaa tagaattggc
    10741 catgacgttc gtctcctgct ttcaggccgt gatgaggtac ttgcgaacgg gcacgccttg
    10801 caggacgtgc ccttgcacga tctcttcgat ttttcgctga tcgagtctgc cgtagcggaa
    10861 ttcggtgccg ctggcgtcgc gcagcgtgat catcggttct tggtcacaga ggcccgcgca
    10921 accagctcgg gtgagggcca cttcgtggag gttttcccgg tcgagttgtt cggcgaaatg
    10981 atggatgata tcccttgcac cggcggcaat gccgcaggtt cccatgtgaa cggtgatccg
    11041 aagcgcctcg gctccctccc ggggcttgag ctgctgacgg acgcgcgctc gcagcgcgct
    11101 cagggcttca cggctgtcga ttcgcttgtc catgctgacc tccgaacttg ctagcatgtg
    11161 attccaacac accttgaact cggctgggct tgacgcgatg atagacctcg tcgtcgatca
    11221 tgatggccgg ggcgagccca caggctccga agcaacgcgc aacttctaat gtaaatcgac
    11281 gatcttcggt ggtaccgccc acctcgacgt gggcggtttt tcgaatggct tcgagtacgg
    11341 ctttgccacc acgcacgtag caagccgtgc ccaggcacac gcggataacg tggcgtcctc
    11401 gtgggacggt tgagaagtag gaatagaagg tcaccacgcc gctgacttcg ctatacggtt
    11461 tatcgagtcc ctcggcgatc cgtcgcatcg ccacttcggg taaatgaccg aagattccct
    11521 gggcaatttg caacacggga atcaatgccc ccggttttcc tcggtattct gctatagtgg
    11581 cgtccaggcg ctcgaggagt tcttcctcgg atacctcgat gccgcaattg cacgagactt
    11641 gtaccatgtc catttcgtcg tcctccaggg ccgcggaacc gtccacggcc tttccacacc
    11701 tcaaggcaaa gcccctaacc cctcgagtcc ttttctgatt tggttcgtca tttgccgcat
    11761 caccaaaagg ttttgaccag gcccctgccc ggtcgcggcg cgaagctgct gagtcgatac
    11821 cgtctctttc tttccgtcga cttccacctc acaggaaaaa tccacttccg gcgccgtaca
    11881 agccattgcc cccacggtgg cggccagatc ccccaggggc attctgtcga tatgaagcaa
    11941 gcgcatacgg acgcgaaccg ccgttcctcc ccacggcgaa gcgcaaaggt gcaggtcgcc
    12001 accggcccgt tcggccgctt ctcgaaacag gctcaaaccg agccccgtac gcttgcctgg
    12061 tttggtggta aaaaacggat gggccgcatc ttgtaccgta tcgggcagcc cgcacccatc
    12121 gtcctccacc gcaatttcga gctggtccgt cgcgcgattc tgcacgaccc gcacgagcac
    12181 ccgctgggcg ccggcagcga cggagttttc catcaaatcc aggacgtgca aggcaatctc
    12241 acgcactgtg gcacctccaa tccgaagctt cccgaagggc ctgggcgagt tcttcgaaac
    12301 acgcggaccg cagagaaacg gtgcaataca cgcttccaat ctcgctcaaa tcgtgggagt
    12361 ccgaccccac caacacgggc aatccgtatt gcgccaagtg caaggtatcg attcggtaca
    12421 agccggaagc cgacagttcg atggcgtcga gcccgacatt ggggatacag cccacttttc
    12481 cgagcaagcc ggaggaagga cgatccacat gagccgcgat ggcgagtcca cccacgttgt
    12541 ggatgacccg gacggcttcc ggcaaggtag gataatttcc tttgcgatgg gcgcgctcgg
    12601 tgttttttcc atgcggaagg ccaagttccc gcgccaaggc cacagcggct tcccaatgcg
    12661 gaaatatgcc gagaagatgg gttttttcca gggtggtgat ctccacccct tgcaaaaccg
    12721 cgagcccgtc gacggaaaaa ggctcttgcg cggctccgat ccggtcgtga tcacaaaaag
    12781 cgatgagcga aagaccacaa tctttggccc gacggaccac ggcccgcgga tccatgctcg
    12841 ggtccgcgca cggcgacagg gtgctgtgca agtgcaggtc gagggccagc gacttcaccg
    12901 cgtacctccg cgcaatccga gcgcgtaaag acgccctgcc acgtcaaacg aggtgcaaag
    12961 ggtgcgaaaa gcgggaattc cctcttcgac ggcccgaagc aatacgtctg gggacggttg
    13021 gactccgcct gtgaagataa ccgcggccag tccgacatgg gacgccacgg ccagcacgtt
    13081 gaggtgggcc tgggtggtga ccagcacggc ccccgcgggc gcatgagcca ggacatcgct
    13141 cagaaggtcc gaggcgtatc ccacggtaac ggggggatcg atggcttggt tttcggcggt
    13201 caggtcctcg aggccgagct gggtagcgat ggtccgaaga gtgagggagg aattcacgat
    13261 tgactcctcg cgctgggatg tttgcattgg agactacgga tccggccgag ggcataatcc
    13321 tccgcatggg agcgacagga aggcgcgcca caacgtccgc aatcggcctg gggaaggctt
    13381 cgcagaactt ccgccatgcg atgcagcttc ttgacggaag cttgcatatc cgaatcgagc
    13441 cgcaaggcag gtcttggctg ataaggggat tgtctgcggg caacacaata ggttgcgtca
    13501 tcattgattg tcatgcgacg ggatcggtgc agggcaagcg cggaaggcgt tcggaacaag
    13561 ggagaaccga agcagccatc gacgcaggcg cgaagcatga cacgttgcgt aaactcgagc
    13621 cgttcgcctt cgatggtttc cagcacacgc aaggtcgagg cgagacccga ggccatcaat
    13681 tcatttcgct ccacggcagc gcggattggg gggcagtctg agtcctggtc ggttgggcgt
    13741 ttcgctcgga gccgttggtc gatggcctgc cgcaaggcgc ggggggtaag gatccgctgc
    13801 gagggcaccc cggcctggcg caagctggtt tgttgggcgg ggcaggcgac gacgaaggcg
    13861 gccttttgcc cttgcagttg ggcggccgcg gcttcgaggg gcgacagcag cggagccaga
    13921 tggggcaaca gcgaggggaa gcgggtttcg atcaagcgag tcacgacggg acaggcgaca
    13981 tcgatgatgg gaagggggtg gttattttgg gcgagcagtt catcgatgac gttttgctgg
    14041 gctgcttccc actgctccaa gagggccagg cgcgaaaacc ccaattggag aattatttca
    14101 cgaattagac gataagagac gttgggaccg aagtccgtga ggaaagccaa gggcaacacg
    14161 agaacatcga gggagccggc atcggggcag ggcgaatcgt tatcgatttg aatggctcca
    14221 tggatgcagt gggcgatgca ggcattgcaa tccacacagc ggtgcgcaag caaggaaggg
    14281 cctgttccgc gcacccgcaa cgcctgggtg gggcatacgg gaaggcatcg gaggcataga
    14341 cggcagcgtt cggacacgaa gcgcagcgca ttgggggcag ggggcaacgg ttcgacgcaa
    14401 gaaaacgaaa ggccgaaacg taggcggcag cccacgccga cggtggattc gagcgagaag
    14461 gtgtcgctat ttcgttttat attgggcaag ccgagcccgg cgccgaagcc gagggagcga
    14521 gcctgctgtg aggcggtgga gaaaccgggt tgcaacgcaa gggccacgtc cgccacgcca
    14581 ggcccctcat cctcgatttc aatgtcgagc cgatcatcac caatcgtagc ccgaaggatg
    14641 ccacgtcgtg catggatgat gacgttgagt tctgcctcat aggcggcaat ggctgccctt
    14701 cgggtgcttt ctggatcgac accgacggtt ttgagcaagt ccttgagggc atcggtggca
    14761 tggccggccc gttcgaagtc accccccaca atggtatggt ggaatacggt catgggcgca
    14821 cctcccgcct ggctgtagga aacagcgcgg aagcgataac ccttctgagg gtaatgcagg
    14881 tgcgcatgcg actctcttgc caatccggag gcggcgaaac ccgcgtttcg gacccttcgt
    14941 cttggattag tgatggtttg cacgacttga ctagcacgcg cccttgggcg agtcaatccg
    15001 agcgaaccac gaggaacagt gatattcttg tagttgtttt tatggcatgc gggaggaccg
    15061 taagaagcgt tcaccgttgc gttccgatat cgtgaattaa tactcgatta tccatccctc
    15121 ctgaccctgc cctttcttgc ccccttcccg ctggccaggg ccccgcttgg tacgataccc
    15181 catcgactca agcctgctcg attgaaccat ccagccttct gtgagacagt cccccgcctc
    15241 agcgaaggag tcgaccatgg ccaccgtacg aacgtggaaa tgcgaagtct gcggatacgt
    15301 tcacgaagga cccgaaccgc ccgaaacctg ccccgtctgc ggtgtcggaa aagaaatgtt
    15361 cagccccctg gagttcgccc ccgaacccgt cgccccatcg ccccccccca aagcctggcg
    15421 atgcacggtt tgcggccata tccatgaagg cgaccatcca ccggaaacct gccccgtttg
    15481 cggcgtggat gccggactgt tcgaacccat cgctccagaa aaacccgtaa catcagcccc
    15541 ccaaggcccc cgaatcgtca tcgtcggagc cggcatcgca ggcgtcatcg ccgccgcccg
    15601 cgcaagggaa acgtcttccg atgtgcaaat caccctggtg gcaaaagaac agggactgcc
    15661 ctattaccgc ctgaacctca cgcgttttct cgccaacgaa gtcaccgaaa acgaccttcc
    15721 cctttatccc gaaacgtggt tcgaaaaaaa ccatatcgaa ctcatccacg gcgattgcac
    15781 ggccatcgat cgtcaaagtc ggcaagtcct ccttcgcgat gaccggcgtc ttgcctatga
    15841 tcgcctcatt ctcgcgattg gttctcatcc ttttgtgccg cccatagctg gcgtaacgcg
    15901 acaaggggtg cattcgttcc gcacgctttc ggatgccaaa gccctggcgg ctctggcggg
    15961 gccggctcgt cgatgtgttt gcatcggcgg tggattgctt ggcttggagg ccgcgggagc
    16021 gcttcggaaa cggggctgtg aggtgacggt gctcgaaggc tttggctggc tcttgcctcg
    16081 acagcttgcg caacccgccg gccagatgct ccaagaccat ctgcaaaagc tcggcgttca
    16141 cgtcatcacc aacgcgcgca tcaaacagat cgatggcgat gaagccgtcc gcgccgtgca
    16201 gctcgaagac ggtaccgaac tgcccgccga cctggtcgtt ttgtcgacgg gcgtgcgtcc
    16261 caactcgttt cttgctcgtc agtgcgggct tgaagtcaag catggcgtgg tcgtcaacga
    16321 ccggatggaa acctccgatg ccaccatttt cgcggtgggc gatctggcgg agcaccgcgg
    16381 tcaagtgccc ggattatggc cattttctta tgcgcaggga gcggtagcgg gagtcaatgc
    16441 cgcgggaggc agcgccgaat tccacggcat gccccgcagc aatcagttga aagtcctcga
    16501 cgtctcgatg gtcagcatcg gtgagttcca gcccgccgac gcaagcttcc gcgtgttcga
    16561 gctgcaagag ccgggaaagt acctgcggct ggtgtgtcgc gatggcgccc tcgtgggtgc
    16621 caacctttac ggcgatgcag ccctatcgtc gttggtgcga gaagccgtgg agtccggtcg
    16681 tcctctcgcc gaacagctcg aattgttgaa acatattccc gcgctgcgaa cgtcgtgcga
    16741 acgtggcgcc taaccagaat cgccagcggt cccaaccgag aacatccaag gaggagtcat
    16801 gccgagtttg aaaggaacga gaacggagaa gaatttgttg gcagctttcg cgggagaatc
    16861 ccaggcgagg aatcgataca cctacgcggc ggggattgcg cgcaaggagg gataccgcca
    16921 gatcgaagct ctatttcttg agacagccga aaacgaacgc gaacacgcca aggtgttttt
    16981 caagcatctc gagggcggaa tggtagaaat caccgcgacc tatccggcgg gagtgctcgg
    17041 cacgaccctc gaaaacctcg aagccgccgc cgctggtgag aatgaagaat ggacggcgct
    17101 ataccccgcg ttcgccgaga tcgccaaaga ggaaggattc cccgccatcg ccgcctcttt
    17161 caccatgatc gcacgcgtcg aaaaagaaca cgaagagcgg ttccgaaagc ttgcggccaa
    17221 tgtgaaggac ggaaaggtat tcaaaaaaga aaagcccgtt cgctggaagt gccgcaattg
    17281 cggctacgtt catgaaggaa cggaaccgcc tggcaagtgc cctgcctgcg cgcacccgca
    17341 agaacacttc gagatcaaag aggacaacta ctaacccccc tcgttcgccc catctcccga
    17401 cgtttttttc ctatccgcat acggatcaca gggctggttg tttttgcaag gccgccccag
    17461 ccgcacatcc aattcgatca cgaattgatg ctgatcgtcc gtcataaaat catcgagcat
    17521 gggcgagtat gtcttgacct gcaccgtttt cccgtcggga agcagttcca acaggcgcat
    17581 atatcctctt ccgcccccag gcatcatctg aaaattggat aacatctgat gcaccgtatt
    17641 gcccacatcc cctttgcttg ccaggtatcc cgcaccatct ccgagaacat gcccgcacaa
    17701 caccatggcg atatttccat gatgactgat gagatcgttc cacagttctt ccccatcact
    17761 gactcccccc ggtagcttgg ctgtaggata atggtgggga ttccaaggct gatcgtcgcg
    17821 gatccgatcg tagcggctgt tgtcatgaaa taggtaggca tgggtaacca cgatggccgt
    17881 tcgatccgcg taggtttgca atactttgtt ggcccaatcg accacgcctc gccgcggccc
    17941 ccactcgagc atcagcgcga tccaatcccg gttccccgcg gagaacaagt gatagctatt
    18001 gtccagaacg cccgcgttat aggtgccccc aagcgcgccc accttgcgca ttttggccac
    18061 gggaaaatgc gtgctcaagg ggcttcgtcg cgtgttcgcc cagcccttgt cctcatagtc
    18121 atggttgccg gttgctatcg catacgggat cacaccatcg agaagcccca tcgttttttc
    18181 ggccgtttgc cattcgcgcg cgctcgaacg ctccgtcata tcccccagat gcaacacgaa
    18241 tcgaatgtca taggtctgct tctgttcgac gatccaccgc acctgccgct cgaacgtctc
    18301 gggatatcga tcggtgtaca cttgcgtatc cggcaacacg gcaagggtcg aggagccttc
    18361 cacgaacggc agaggcggag gaagcgggca cggcagattc tcgaaccatt tcgccaggat
    18421 ggcggcgggt cgacgttcgg gctcgggctc gggcgctgac tcgactcgac tagaaaccgc
    18481 tttggactcc cccatgctcg agccagagca cccgggcaag ccaacgatga gagtcgcgac
    18541 cagtgcccac cggaagtagc ttccaaagat cgtgggcttg cgtaaccttc gaacgttcat
    18601 cgctccttct gtttccaagg ctttaatcat tgtcccacgg tatctctggc gcaaggctcg
    18661 aaagccggat ggctagcgct catgatagcc gtttgttcac ggcaaatggt atggaatccc
    18721 cgatgcaaaa acccgtttcc atgaccgtcc tgggcgtgtt gaacctcgtg tttgccttga
    18781 taggactgtt gagcacgatc gcatccacgc tcgtgatgct gagccaagcc accactaacc
    18841 ccgtgtatgt catcatggcg gccaatccaa cctaccgatt ctacgtgtac gcaagcctct
    18901 tggtcggtgt ggcccttatc agtttgctcg cggcgggagg catcggtctt ctgctgtcga
    18961 aggcctgggg gcgcatctgc acgatactct acgccattgg caccatcttg accggtctca
    19021 tctccgtcgt tgtcaactac gttttcttga tcgctccgct ggtggaacga agcgctcata
    19081 caaagacgcc agaggaaatt ggcgccttgg ggggagccat cggcggcacc ctcggtggat
    19141 gcttcggtct cctttatccc gtaattttgc tcatattcat gtatcgcaag aacgtggtgc
    19201 agttcttcaa tccctcggaa acgacaccct catcggacgc tccggcgtgg gaatacgatc
    19261 gtcggtagga tccaccaaca cgaccaatcc cagcatcacc gaagagcatc gctgcaccca
    19321 cgcatgagcc acgtggtcct gatgacccgc cgacgatcca aaggcgatgg ttgcggctcg
    19381 ccatccctga aacgaagcag aatcctcgat gtcctgcacc acaatcgccg aagcgggaat
    19441 cgtgagtcca gtaccgaggg ccttgagtcc cgcctctttc aacgtccata tctctgccgc
    19501 gcgccagcgc ctctccagcc catcggcgcc cttgcaatac gcctgctctt tgggctcgaa
    19561 gtaatcggtt gcgatcgctg cgggctgctc gatcggatgc tccacatcca cgcccaccag
    19621 ccagggaagc ggcgccagtg cggcgctcgc tacccctttc gtatggctga tcgacagcga
    19681 aaacggcatt gttccatggc gatcatgttc gatccacggc acgcctcgtg catcggcaac
    19741 aatcgccaca tccctaggat caagaccagt cctacggtga acgagagcct tcgccaccgt
    19801 tcggccgcga agaaaatcat cttttctttt gggtatggcg cgaggactaa gcgattgcct
    19861 ttcgcggggg tgtagaccgc tttgggattg aacctcacct tcgagaatgg ctatccatat
    19921 cgggatagat actatcatga cgggcaggcc aaagaccttc agggcgcggg ctcgaacccc
    19981 aggagtccac cgccgagggg cgcccacacc tgcggatcca tttgctccgg caacgccgag
    20041 gttcggtaac ccaccacctc gagcaaaatc ttaccggaat cgctggtgac cacggcatcg
    20101 aagcgcaacc catcggaacg cgaacccatg accacccgtg cggtccaagc cccagattgc
    20161 tccgctcccg caaagacccg aacctgctcg attttcgacg gaagtccgag tttgcgagtc
    20221 gatccaatct cgatgacacc cgccgtctgg aaacacaatt ccagcaaacg cggcgccgac
    20281 agcatcgaac gcggttcgcg cgtcaaatca ggcaacggat gcatcatctt tcccaacagc
    20341 gtagcgccat cggcacctac ccgggtgcct tccagcactt gaaaggccgg cccgtggaag
    20401 taggcccgat acaaatcttg ccgctccaca tttccaccct cggctttggg cgacaacgcc
    20461 tccaccgtcg ctccctcggc actttccgaa gcgagttcaa gaatcgccga gaaatgcagc
    20521 ttcggctccg cggccttctc agaacctttg atgctctgta tcgagtgcaa cgagcacacg
    20581 gctctatcac catccgcacc gcgaagaaga cgaaccgtga cgatggcctc ccgcggctcg
    20641 ttgcgataac acttcagcgg cgcggcaaat cgaacgtcac ttaggcttcg aaccgagatg
    20701 ccaggcatca gcacggcaac cgcctgggcg aacaattcca gtcccatgac gccgggcatg
    20761 accgccaccc catcgatcct gtgatgatcg agataaggtt gctcaggctc cagcatcgtc
    20821 gtcatcacga cgccttcgta cggatcactt cgcaacacct tgaaagcaaa cggtcctttt
    20881 tccgacgtgg gcattggttc cgaggatttc gctccgccgt cggcgtcccg aggctcggtc
    20941 aggatgccca gcgccccact tatgacgact tctccctgtt gtccgccttg taattgctga
    21001 cgaaccacgg gcaccgcgag gtcaggatcc aacaattcga ttccagcccg cttcatgatc
    21061 tcgggaatgc tgccgcgcgt ggccattccg atcgatcccc atgccgtcca atcgatgacg
    21121 atgccgcgcg ttttcggacg gagccaggtc atcgccgagg tgactttgca taggaaatcg
    21181 tttgcggaac tgtagtcggt ctggccggca tttccgaatc gtccagccac cgaagaaaac
    21241 gatacgatgg cgccaacgtc gacatttcgt gtggccgcca acagattgaa catccccatg
    21301 accttgacgt cgaagaccag attgaattca gccggttctt tcgtgtcgag gccgcgactt
    21361 cgctcgagtc ctgccgcatg aacgatgagg tccagacgac cgtgtcgctc gaggatgtct
    21421 ttcacaatcg cttcgacttg ctgcggatcg g
//
LOCUS       DAOSOR010000027        21150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_2501_length_21150_cov_5.251007, whole genome shotgun sequence.
ACCESSION   DAOSOR010000027 DAOSOR010000000
VERSION     DAOSOR010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 21150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21150
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2501_length_21150_cov_5.251007"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PK710_02650"
     CDS             complement(<1..426)
                     /locus_tag="PK710_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08637.1"
                     /translation="MVQPIALCIENLDAHTPKDRYVCCVATPGYDIALGLDEKGGVIW
                     REEERLAHELWVSADQQLMLVRRTGRPAIVVTRAGRSLSLPYDKPVVLLDCDRIDVQG
                     VHLRIHIHGETDQVVAPSFWEPERSAGTVAAIATAVAISA"
     gene            complement(504..1070)
                     /locus_tag="PK710_02655"
     CDS             complement(504..1070)
                     /locus_tag="PK710_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08638.1"
                     /translation="MPWWSQFFPQRSLKGITTATRVSLQATVGGRHLVTSFLTGVRAV
                     YFQYTFFREVVAKDAADKVMYIDIGTQSHGTNMQLECDDGIVEIDPNRMIVAFVGAQG
                     HGVLLNQPLPASMAAINEKIGVSGQVFVREMNLTVGEEVTFRGIVEPLRGDSPQGPFR
                     QAPHDLRPRFRVCGKRGPAHLTQKLRLG"
     gene            complement(1180..3747)
                     /locus_tag="PK710_02660"
     CDS             complement(1180..3747)
                     /locus_tag="PK710_02660"
                     /inference="COORDINATES: protein motif:HMM:NF023454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HOT08639.1"
                     /translation="MSDTSFPLVRILSRLSQRGSNVDRDELLSLFLDYVSELGFDLYP
                     AQEEAILELLQWHHVILNTPTGSGKSLVAMALHFQALAENRVCYYTSPIKALANEKFF
                     DLCETLGPENVGLMTGDGSVNRDAPIICCTAEILANRALREPDFPVDYVVMDEFHYYG
                     DRERGMAWQIPLITLRDTVFLLMSATLGDVSEIAKRLDAYTDRQVSVITGAQRPVPLT
                     FSYREAMTHETVQELFDSNEAPIYLVNFTQRACAEQAQALTSLSFLSKQERQQIAAEL
                     QSVRFDTPNGKELSRFLRAGIGIHHAGLLPKYRLTVEKLSQAGFLKVVSGTDSLGVGV
                     NIPIRSVVFRQLCKFDGDRTAILTAREFHQIAGRAGRRGFDDHGLVVVQSPEHIAENR
                     RLEAKRIKNPHLKNKLVNKKPPIKGYVPWDEKTFDRLREAPPEPLVPQFSVNHGMLIQ
                     VLQAYAHVPGGGYRRIVEIIQRAHLRDAEKRIQLRLAAKLFMSLRQVGVVQVEWEDNE
                     PLRFVRVREDLQKDFSLHQALSLFLVEAVELIDPTTETHALDVLTLVESILENPTVVL
                     LKQVDKLKGELVGKLKAEGVEYEERMEALERVEYPKPNAEFIYETFNLFAKGHPWVGA
                     ENIKPKSVARDMVERAVTFNEYVRDLGLARSEGVLLRYLTQVYKTCVQNVPETLRTEP
                     FDDVISFLHTMIIRTDSSLIEEWERMMRGEIVRPKDRFQPPEPTSPTHLASDFKAFVR
                     RIRNEMFMLLKSLSDRNWEDAVTCIRDDGDEPWTEKRFEQVMAPYFDAHAFIDVSPSA
                     RGTRNTEVQQQGEQCWSVLQRIIDPEGDEDWFIEGWIDLDLIRDQSEPLVSLRRIGS"
     gene            3965..4411
                     /locus_tag="PK710_02665"
     CDS             3965..4411
                     /locus_tag="PK710_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOT08640.1"
                     /translation="MRVKMTLEDVLQGKTKGGVVTADESTLMREAVKMMATNNFGALV
                     ITSEGRPAGIVTERDVLRQAAQGEGFLNKPVSEVMTRDIVVGRFSDDVEVAKRVMTDK
                     RFRHMPIMQKGKLVGIVSIGDVVRSQLMTIEAEATYLRDYIRGEYG"
     gene            complement(4425..5429)
                     /locus_tag="PK710_02670"
     CDS             complement(4425..5429)
                     /locus_tag="PK710_02670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT08641.1"
                     /translation="MLPLVSVLFPYRNAAETVEQALDSILCQTYEHLEVCAVDDGSLD
                     EGASIVAARARRDSRVRCLSTDGRGLVAALQHAHRFSHGEYIARMDSDDISLPTRIER
                     QVQWLRANPKVGVVGVQVEGFPTDDLGEGMRRYIDWQNALVTPQQHAQEIFVEAPLCH
                     PTALLRREALEQVGGWIDTKGPEDYDLWLRLDQAGWQLAKVTEVLFRWRHHAGRATFS
                     ESRYALARFRETKAKYLAHRIRSLAMPMVIWGAGPTGKRLCRELESHGLRPRYFVDID
                     PRKMGREARGIPIRSVQELRSGRETIVVAVGARGARNLIRDFLIPRGFVEGKHFLFAA
                     "
     gene            5712..6767
                     /locus_tag="PK710_02675"
     CDS             5712..6767
                     /locus_tag="PK710_02675"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOT08642.1"
                     /translation="MNKPRYYFINERKHASLDFATMGFDYLACPWRFEAVFQHGIWRV
                     RGIVEDTTMHLPEGSQCLHYGQELFEGMKVQTGADGKLYAFRPLDNARRLNAGADYLC
                     GPPVPEELFLRGVEEVTQANAPYVPPYGTGASLYVRPFYLGIGDNVGVKPASSYVFRI
                     FVSPVGPYFKGGFGPEKGQKFRVSQYDRAAPHGTGHIKAGGNYACSFFPGRDAKSQGY
                     AEALYLDPQEHKYIDEIGAANFLALKKNTLLTPKSTSVLPSITRRSLMRIASDLFQWP
                     TEERKIALDELDEIDAAACCGTAAVITWIQEIHDSDRKWQFPFDERWKKLYDKLVGIQ
                     TATEEDPFSWRYEIKLG"
     gene            complement(6798..8315)
                     /locus_tag="PK710_02680"
     CDS             complement(6798..8315)
                     /locus_tag="PK710_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT08643.1"
                     /translation="MQSISNLPCQAPADHRSTNHEACVSTAFDAWFQVLGDRPLLSRE
                     AEVALAQQMENGERVMFHALLRTRLVGQELARLLERLEGGRVRLSRVLRDGDDNDEAA
                     RKHLMSVLKAFSALHKAREQGLKAHRTVGSSGMPSTWDDQMVAIFEDIRWDRQQIDRL
                     DHKVTTVQRQIEDARRGIRACEKMASMPYQEVIATVRAWKAQADEGCILGRRGMRMDE
                     LSSMSDTIRHALEVIEAGKRSLGVSEEVLEQIMAEVARGRAIAQRAKVTLVESSLRLV
                     VHTAKKYAGRGVDLLDRVQEGNLGLLRAVDKFDYRRGYRFSTYASWWIQQAVARSIAD
                     QGRTIRVPVHMFEALTQLRRLQKQITHEHGYVATADELSQRSGIPLDQVHKALSVVED
                     PVSLDAPVRSEDDRLLVEFVQDARSESPKDALVRDRLGVVTRQMLATLTPREQQVLRL
                     RFGIDEQQDWTLEEIGKRFDVSRERIRQIEARALAKLRHPKCAAQLEAYLTGSVL"
     gene            complement(8520..12593)
                     /locus_tag="PK710_02685"
     CDS             complement(8520..12593)
                     /locus_tag="PK710_02685"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT08644.1"
                     /translation="MSILKRTKTIIGHVFLVVMAYGSTAHAFLPSDFNPEGRRTPRPG
                     PPTQRPASSTPRASSDAGVGTDALIKRYFAIVMARPDEVFPLRRLADLYRQRDGNLAQ
                     LITELEQRVAAGGTEAWSARVALAGVYRLDNKKAEAIRLYREAVQQRPNALGAVRALA
                     EAVLDDGDLAEARGLFDQVLANSTERVDKEAALRTLIRISLDQKDHTAAKRYHEQLVS
                     LAQGSIFVRAELGREYLQRDEFALAEAEYRQLVKAAAGDNRALAPALRDLGQVLVRQR
                     KTAEAIEVLRRALQVAGQGAGVRREIHALITEAYRAEGKLPELIVLLESSRTHDFDEV
                     VTLGLLLEETGQVDRALDVYRRALHLNPRHIDTHLKIIHLLQAQGELDQAIRQFEALI
                     RAVPGNPDFSLELCEILIQRGDRAKALALLTRLEQQIRDEDQLGRLADFYERIEEKDR
                     ATRLLQRLAQGGGRDPRYIIDLGDRYFQQGDKKKAMETWARLRTVITNRAEALATLGE
                     VYLDHDMTNEALEALREAVSLEPNQLRFLKTLAIALERTGSTMTRRRVLTGAHAEALQ
                     IWGKILDMAYRAQDEAQAREARVHIVSLWALSDQLGAQVSPLSAKFSANPPDIQAGRM
                     LVEVQIRLRQLAEAEQTLSRLTQLRPGEADLFLTLERVYVLQRKIDEAIDVLAKLTSI
                     DPKRARQYYQRMAQYAAELYRDDDAVKYAAQAVALAPDDAEGHRKLGEMYRKRQDTEQ
                     AVAEFRAAITKNDRLFLVYFDLAELLLSKGQTDEADQLYRRVVRLCPDEEMVARAARL
                     SMQINLGKNSLEVLERELLPTALGNPQKRVYRRLLVDLYGVMTFPLVQDVRYGSKASS
                     LAAREQLSKIGARAVKPLLDALADENEAQQRIAIEVLAFVQNRSAAPALFAFATGSAE
                     PELRSRAMLACGALRDPGLLPRYASILSPTDDSSVLLGGSIALAATWGVARSGDPGAR
                     PVLRQLAKQGSPEIRALALLGLGFDKGSQSAALLAETTRSVEAGNVAIAAAALGLSEA
                     GATDRASALVAVYRSSDPLPQQAALVALARLAPERARPLLTGFFDPNVATRKATLAAA
                     LVVATGQFKRSIDPWEVPAGQLDVHALLDHLMPSGYDRSDYARVLVELEEQVGASALQ
                     AVRSSAEGARLVADALLSYDGDYALAPFTDGVDKLDPDFRKRVRAAAQRVTARVEPAF
                     LSLVRHPSVDLRTRTIRVLARQNTAQSRAAVIDALEDDNELVQRAALTALGEQGADEG
                     IQALCKTLLEGSSWSLRLRAARALGLLNPENTSSQAVQALSRAARQDSFALVREASLT
                     ALVRIAHSQAQPIVREAAASDQEPAIRKLASQLLQSY"
     gene            12762..13655
                     /locus_tag="PK710_02690"
     CDS             12762..13655
                     /locus_tag="PK710_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT08645.1"
                     /translation="MKRNLVAFSSVVALGLAMAACGYSEDEWQAQLAKYNAEVAKNKA
                     ANQRVADLEQQLEASKNRVAGLEMQLEAMGLDINKLNTALADRGQALADLNADMDKMR
                     KALTEYQARAATLERIKERMLTLRKKLESLTRLGLNVNIRKNRMIISLPGDILFDSGK
                     TELKEEGKNVLREVAKVVRNDQTLLARDFQVAGHTDNKPLKGGPYLDNWGLSLMRART
                     VLVFLVTPTDIEPSGYGANRIEPGGGLPTSRWSAAGYGETDPVAANDSPDNMQKNRRV
                     ELIVMPNVEEMLDLKSLTAVP"
     gene            13731..14174
                     /locus_tag="PK710_02695"
     CDS             13731..14174
                     /locus_tag="PK710_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08646.1"
                     /translation="MFDRTDRQFFAGQDVQGVVTPLQRVLHSQGIPLQQTGATSWSGR
                     GTVPSWGLVPKIHINAHPSQQGFFLDIRVFADVEGNGIIAFILAWFFFFPVAIVLGFV
                     AHNNFVQQRDYLIHSLWSPVGHLIATPNYPPVPFGAPGGAPFPGP"
     gene            14192..15178
                     /locus_tag="PK710_02700"
     CDS             14192..15178
                     /locus_tag="PK710_02700"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOT08647.1"
                     /translation="MSPTVVVLIVLFALGTALAIFVFTRKRSTAADTRHDEPPKSLQP
                     QAVLSVIPHEQLEQEAEEIAPTDYELRIFGVGRTDVGLRRKLNEDAFVCLPEHELYAL
                     ADGMGAHAAGEVASKLTLEAISDAFEHNRYLSAESSEQPSHRKMRLVRILERANQIVW
                     TMSNEVQAYRGMGTTVVMAHFSVHKRYVFIANVGDSRCYRIRDGQLTQLTTDHTLGSV
                     GVLGKTSSLLSRAVGIEPNVEVDIMTYRTKPDDILLLCSDGLSRMVADDQICATVMDH
                     PDLNVAADALIAKAKDAGGKDNITAVLIRMEKLPPSNHEGDSGLRDRTTLPV"
     gene            15252..16736
                     /locus_tag="PK710_02705"
     CDS             15252..16736
                     /locus_tag="PK710_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08648.1"
                     /translation="MSHPSAHQEPPNISGVLGSLALSAIRPGSSTLALRALRWFRDLE
                     RLGLRLPFFVIHDMGLLFAAGSEHYAIGPRCAPQMLLGILSERYDLSNTYVSLIREMA
                     TCEAARRAASLHLSDDMVSVLLSKLLFSLSANPSFKPKYPTDLVPDASMFERIEDGLV
                     ELYRRFDRSFELQTLHRLTNSRLLLLTIADALDLDTLQLLGVLGAEAGTEAGLQVDLL
                     SAMATPAAHDVVNFSLDILPSVLESKTRPGASERAGFGCGGLGTRGSIDNLVLTELAW
                     DADDFTRRVIDNEVLYYTKETEREPAGRRHILLIDASASMRGERQVFARGMALATAKK
                     LVLEGEDVSLRFFDARLYEPQSARAGRLPTPFILSFKGERGRNPQRVFSDLITALSIE
                     KIRDQRDVVLHVFTHAALYIPRRIVENLVRIAQLGIVFILPSGGSLDLDYLDLLHAHF
                     VVDHDTLASRGARAEKARDILGQIGSQSPQSPMVAVSHSMGETS"
     gene            16733..18589
                     /locus_tag="PK710_02710"
     CDS             16733..18589
                     /locus_tag="PK710_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT08649.1"
                     /translation="MIGGDPDVARTIAQAQHDIRVGNLSAALEILLQSLNQTYVTDQD
                     YILALRHLVDVFGRLGDARRAITCAWYLDDDRLAESLMPRVTAEDRARTYIAQALRWP
                     SGNSRRAELFAHAAREFESVGCLAQAAVNLERAGLFRDAHPLWLRLCHTIALHDEDDI
                     YAAGLAYFNLARTAKRDGNQKEAQAATVTSVHLLEEAADRFEQLGQRERAFDCYQVLV
                     AIGRETETIEHVLEGYVNLTRILREDHLRTYALKEYQAAIQFLRERSEIAAAATLCRE
                     MADYARRQGLLPLANRAIIDQAGMWRELATTVVQRGQGPEIAENALLAAVLSYAELGQ
                     FKLAGNVYVELAHLPLEPSRRNHYARAAQRYQNAQDTSIDLGQLPNAVRGDSEFPDVW
                     HVDVIEWEQRGVVSEVCADVMLDRATWSEVIRRRAMVARMTALPCEMAPHAPIGAWLQ
                     VTENLAQTELYATIAPLEKMYRRPEPEIRAAVIKALGRFLFKRSFITLRRALLDPELT
                     VRTQAWNAIRQLRFPHAFDPLARIYRESTDLSAQQAALAALAMVDTEEAAEFMLSVLQ
                     QVGSSERSLIDSWIRSCGPRLIQAARDALPSASGDFADLLRFLLNSQTSRLR"
     gene            complement(18782..19021)
                     /locus_tag="PK710_02715"
     CDS             complement(18782..19021)
                     /locus_tag="PK710_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08650.1"
                     /translation="MIKGVVTIDPLYASRRRQKQIAEVLEAHGFRCELRERFDRLHIV
                     AFEIEALPGDPNEEIYRIVHESNGWHEKDCKIRIL"
     gene            complement(19072..19767)
                     /locus_tag="PK710_02720"
     CDS             complement(19072..19767)
                     /locus_tag="PK710_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08651.1"
                     /translation="MSPNDSEYRFSRDLPQSRRGNPRGQSPDSERRSPVSFRTSALAF
                     LDGALERWGIHHLVTWMDQCLIRPGRLLTRHPFPRVTVWERGLGELSYGNLSGHADPA
                     LYLPHGKASTLIDATRARVFDTLSGLHDGTPDDRFVQAAIYDGRVVRRRQPSGQRSWE
                     VMLTERHSLSDQILALLSADFLDHRQDYEHDLVLCGVCKRIEFWPERLSRRACHQHPE
                     ELFSVAHRKTGSP"
     gene            complement(19803..20555)
                     /locus_tag="PK710_02725"
     CDS             complement(19803..20555)
                     /locus_tag="PK710_02725"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HOT08652.1"
                     /translation="MRCRKTNHHVSWSTSPFHPSLVSDEELVACLLALRPGGGSRQRA
                     ATLLKQVGGLAGLARIGPCYAPMTGVTANQVYRLAAALELGLRCAEPSLTPTSVVTCA
                     EDIVAILGPRFRLLQHEQVWLLGMNARNVVTSRWQVAKGGQHGCALLARDVLRVAVQA
                     GAQCFVLAHNHPGGDPRPSVEDVTLTEKLAVAALCIGIPLLDHVIVAQGGHVSMLQMG
                     LIPEVEQEATRGAVPRHASFRMDHDESVHGAD"
     gene            20899..>21150
                     /locus_tag="PK710_02730"
     CDS             20899..>21150
                     /locus_tag="PK710_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08653.1"
                     /translation="MATSFDPTTAIEHLSSISRRNPGAKHATPRLDDVTRLVHGELAS
                     VELWLKDAMARESSCSIAASDLIRAGGKRMRPSIVLLATR"
BASE COUNT         4816 a         6527 c         5351 g         4456 t
ORIGIN      
        1 cgcgctgatc gctaccgccg tcgcgatcgc tgccaccgtc cccgccgacc gctccggctc
       61 ccaaaaagaa ggggcgacca cctgatcggt ttcgccatgg atgtgaatcc gaagatgcac
      121 gccttgcacg tctattcgat cgcaatcgag caatacgaca ggcttgtcat acggaagtga
      181 caaactgcgg ccggcacgcg tgaccacgat cgcaggccga cccgtacgcc gaacgagcat
      241 gagttgctgg tccgccgaaa cccataactc atgcgccaag cgctcctctt cccgccaaat
      301 cacgccccct ttttcatcca aacccagggc aatgtcataa ccgggcgtcg caacacaaca
      361 tacataacgg tctttgggcg tgtgcgcgtc caggttttca atgcacaatg ctatcggttg
      421 aaccatcccc ccatgatacc tccaatacga agccaaaccg agccggtttg ccgtacggca
      481 cggccatcaa gaaatgcaca tcctcatccc aaccgcagct tctgcgtgag atgagccgga
      541 ccgcgcttcc cacacacccg aaatcgtgga cgtagatcat ggggagcttg ccgaaatgga
      601 ccctggggtg aatcgcccct caagggttca acgattcccc ggaaagttac ctcctcaccc
      661 accgtaaggt tcatttcccg cacgaaaacc tgccccgata cgccaatctt ttcgttgatc
      721 gcggccatcg acgccggtag cggttggttc agcaataccc catgaccctg cgctcccacg
      781 aacgcgacga tcattcgatt cggatcgatc tccacgatcc catcgtcaca ctcgagctgc
      841 atgttcgttc catgcgattg tgtgccgatg tcgatataca tgactttatc cgccgcgtct
      901 ttggcgacca cttcgcgaaa gaaggtgtac tgaaaatata cggcacgaac tccggtcaaa
      961 aaactggtca cgagatgacg accgcccacc gttgcttgca aagataccct ggtcgccgtc
     1021 gttatcccct tcaatgatcg ttgtggaaaa aactgactcc accagggcat cggagcctcc
     1081 aaaaccgcgg aaaaatccca gggcggttga gtcgtatcta tgtgaaatta ttagactatc
     1141 ctgattgtct ccacgattct cttctgctgc ccctcgtgtt cacgagccaa tgcgacgcaa
     1201 cgataccaac ggttcgcttt gatcacggat gagatccaga tcgatccatc cttcgatgaa
     1261 ccagtcttcg tcgccttccg gatcgatgat tcgctgaagc acgctccagc attgctcgcc
     1321 ttgttgttgc acttcggtat ttcgagttcc ccgtgccgaa ggggatacat cgatgaacgc
     1381 atgagcatcg aagtacggcg ccatcacttg ttcgaatcgt ttttctgtcc acggttcgtc
     1441 cccatcgtcg cgaatgcacg tcaccgcatc ctcccagttg cggtccgaca gggatttgag
     1501 aagcatgaac atctcattac gaatgcgacg gacgaacgcc ttgaagtctg aagcaagatg
     1561 agtgggggaa gtgggttccg gaggctggaa ccgatcctta ggacgcacga tctcgccgcg
     1621 catcattcgc tcccactctt cgatgaggct cgaatccgta cgaatgatca tcgtatgaag
     1681 aaaagagatg acatcgtcga acggctccgt tcgcaacgtc tccggaacgt tttgaacgca
     1741 cgttttgtat acttgcgtga ggtaccggag cagcacgcct tccgagcgag cgagaccaag
     1801 atctcgcaca tactcgttga acgtcacggc tcgttcgacc atgtcacgag cgaccgactt
     1861 gggcttgatg ttttcggctc ccacccaagg atgccctttg gcgaataggt tgaacgtttc
     1921 gtaaatgaac tcggcgttgg gtttgggata ctccacacgc tcgagggctt ccatgcgctc
     1981 ttcgtattcg acgccttcgg ctttgagctt gcccaccaat tctcccttga gcttgtccac
     2041 ctgtttgagc aacaccaccg tgggattctc gaggatcgac tcgaccagcg tcagcacgtc
     2101 gagcgcgtgg gtctccgtcg tgggatcgat caactccacc gcctccacca aaaacaacga
     2161 cagcgcttga tgcagcgaga aatccttctg caagtcctca cggacgcgaa cgaatcgcaa
     2221 cggttcgttg tcctcccact cgacttgcac cacacccacc tgacgcaaag acataaatag
     2281 cttggcggca agacgaagct ggatccgttt ttccgcatcc cgaagatggg ctcgttggat
     2341 gatttccacg atccttcggt aaccgcctcc aggaacatgg gcgtaggcct ggagcacttg
     2401 aatgagcatg ccgtggttga cactgaactg cgggacgagc ggttcgggag gagcttcgcg
     2461 cagtcggtcg aaggtcttct cgtcccaagg aacgtaccct ttgatgggag gctttttgtt
     2521 gaccaatttg tttttcagat gcgggttttt gatccttttc gcttcgagcc gacgattctc
     2581 cgcgatgtgc tcggggcttt gcacgacgac caggccgtga tcatcgaatc cgcgacgacc
     2641 tgcgcgtcca gctatctgat gaaactctcg agcagtcagg atcgccgtcc tatccccatc
     2701 gaacttgcaa agctggcgaa acaccacgga tcgaatggga atattgaccc ccacccccag
     2761 actgtcggtt ccgcttacca ctttcaaaaa cccggcttgc gatagctttt cgaccgtaag
     2821 gcgatacttg ggaagcaacc ccgcgtgatg aattccaatc cccgcacgca aaaaacggga
     2881 tagttccttg ccgttcggtg tgtcgaaacg aacactttgc agctccgcgg cgatctgttg
     2941 acgctcctgc ttggagagaa aagatagact ggtgagggct tgggcttgct ccgcacaagc
     3001 acgctgcgtg aagttcacaa gataaatcgg cgcctcgttg ctgtcgaata gctcctgaac
     3061 cgtctcgtgc gtcatcgcct cccgatacga aaaggtcaaa ggcaccggac gctgcgcgcc
     3121 cgtaatgacc gagacctgcc gatccgtata cgcatccaat cgcttcgcaa tctcactcac
     3181 atcccccaac gtggcgctca tgagcaaaaa aaccgtgtcc cgtagtgtga tgagcggtat
     3241 ctgccacgcc atcccccgct cccgatcccc gtagtaatga aattcatcca tcaccacgta
     3301 atccacggga aagtcaggct ctcgaagcgc tcggttggct aggatctccg ctgtacagca
     3361 aataatcggc gcatcccggt tgaccgaacc atcgcccgtc atcaacccca cgttttctgg
     3421 acccagcgtc tcacacaaat caaaaaactt ttcgttggcg agcgctttga tcggcgatgt
     3481 gtaataacac acccgatttt ccgccagcgc ctggaaatgc aacgccatcg ccaccagcga
     3541 cttgcccgac cccgtgggcg tgttcaaaat cacatgatgc cactgcaata actcaagaat
     3601 cgcctcctct tgcgcagggt ataaatcaaa accaagctcg ctcacgtagt cgagaaacaa
     3661 gctcaatagc tcgtcccgat ccacgttgct tccacgctgg ctcaagcggg acagtattcg
     3721 taccaaagga aatgacgtat ccgacatgct gggtccttgg cttgcgccac catcatgcca
     3781 gacccaccgc cttcacgtga ccccaacgtt tgcctcgccc acaactcctt accctccgtt
     3841 cgcttggaac ctccccagga ccatctccgc ttccttcttt ccccctatca cgccaaaccc
     3901 ttgacacttt caatcgtgcg aaccaacgat atgttcacca attgctcgtc aggagggaac
     3961 ggccatgcgt gtgaaaatga cgctagaaga cgtgctacaa gggaaaacca aaggcggtgt
     4021 ggttacggcc gacgagtcca ccttgatgcg ggaagccgtc aaaatgatgg cgaccaacaa
     4081 ttttggggct ctcgtgatca cctcggaagg cagacccgcg ggaatcgtca ccgaacgcga
     4141 tgtgttgcgc caagccgcgc aaggggaggg gtttttgaac aaacccgtat ccgaggtcat
     4201 gacccgcgac attgtcgtgg gaaggttttc cgatgatgtc gaggtcgcca aacgcgtgat
     4261 gaccgataag cgatttcgac acatgcccat catgcaaaaa ggaaaattgg tgggaatcgt
     4321 atcgattggc gatgtagtgc gctcccaatt gatgaccatc gaagcggaag ccacctactt
     4381 gcgagactat attcggggag agtacggctg agggaaagat cggttcaggc cgcgaataga
     4441 aaatgttttc cctcgacaaa ccctcgaggg atcaaaaaat cgcggatcaa gttccgtgcg
     4501 ccccgggctc ccaccgcgac tacgatagtt tcacgcccgc ttctgagttc ctgcaccgag
     4561 cggatgggaa ttccacgagc ctcccgaccc atttttcgcg gatcgatatc cacgaaataa
     4621 cgcggacgca atccatgcga ttcgagttct cggcaaagcc gtttacccgt ggggcccgcg
     4681 ccccatatca ccattggcat ggcaaggctg cgaatgcgat gggcaagata cttggctttg
     4741 gtttcccgaa atcgcgccaa ggcgtatcga ctctccgaaa atgtcgctcg accagcatgg
     4801 tgtcgccatc gaaagagcac ttctgttacc ttggcgagct gccaccccgc ttgatcaaga
     4861 cgtagccaca aatcatagtc ttccggccct ttcgtgtcga tccatccacc gacttgctcg
     4921 agagcctcac gacgaagcaa tgccgtggga tgacagaggg gagcttcgac gaaaatctct
     4981 tgggcgtgct gttgcggggt aaccaacgcg ttctgccaat cgatgtacct tcgcatcccc
     5041 tccccaagat catcggtagg aaacccttcg acttgaaccc ccacgacgcc caccttcggg
     5101 ttggcgcgta gccattgcac ttgacgctca atacgcgtgg gaagcgaaat atcgtcgcta
     5161 tccatgcgag cgatatattc accgtgagaa aaccgatgcg catgttgcaa cgccgcgacg
     5221 agcccacggc cgtccgtcga caaacacctc acgcgtgagt cacgcctcgc ccgcgccgcc
     5281 acgatcgatg ccccctcatc caacgaacca tcgtcaaccg cacacacctc caaatgctcg
     5341 taggtttggc aaagaatgct gtcgagggct tgttcgaccg tttccgcggc gttgcgatag
     5401 ggaaacaaaa cgctcacgag cggaagcatg aaccaagctt aggtacgccc tggccccacg
     5461 gacaagcaca accactgcct cttgcttcga gaatcacgaa cgtgcgatga gggcgaccgt
     5521 cgcacggttg tcgtcgaatc ccggggttgg caaccagcac aaggcaggac tatcgtccct
     5581 ttggctcacg tgaagtctcc aaaccatacg ttggcgacag actggccgtg tcgagcgtga
     5641 gcagccttgg tgtcatggga aacttcgttt tccgtgtttg aaatgcatcc tcgagttcac
     5701 aggacgtcag catgaacaag ccgagatact atttcatcaa cgaacgcaaa catgcgtcgc
     5761 tcgacttcgc caccatgggt ttcgattatt tggcttgccc gtggcggttc gaagccgtgt
     5821 tccagcatgg aatatggcgc gtgcgtggca tcgtggaaga tacgaccatg cacttgcccg
     5881 aaggcagtca gtgtttgcac tacggccagg aacttttcga gggaatgaaa gtgcaaaccg
     5941 gtgccgatgg aaaactatat gccttccgtc ccctcgataa cgcacgacgt ttgaatgcgg
     6001 gcgcggacta cctctgtggt ccgccagtcc ccgaagaact gtttctccgg ggagtggaag
     6061 aagtcacaca agccaacgct ccttatgtgc ccccctacgg gacaggagca tccctgtatg
     6121 ttcgtccgtt ttacctgggc ataggagaca acgtgggggt caaaccggcc tcatcctacg
     6181 tcttccgtat ctttgtaagc cccgtgggac cgtacttcaa aggtgggttt ggcccagaaa
     6241 aaggccaaaa attccgcgtg agccagtatg accgggcggc gccgcatggg accggtcaca
     6301 tcaaggctgg tggcaactac gcgtgctcat ttttcccagg tcgagatgcc aaatcccagg
     6361 gctacgcaga agcgctgtat ctcgatccac aagagcataa gtacatcgac gaaatcgggg
     6421 ccgcgaactt cctggccctc aaaaaaaata cgttgctcac gcccaaatca accagcgtgc
     6481 ttccctccat cacccggcga tcgttgatgc ggattgcctc cgatctattc caatggccga
     6541 cagaagaacg aaaaatcgcc ctcgacgaac tcgatgaaat cgatgcggca gcctgctgcg
     6601 gaaccgccgc ggtgatcaca tggatccaag aaatccatga ctcggatcgc aaatggcaat
     6661 tcccctttga tgaacggtgg aaaaaacttt acgacaaact cgtcggcatc caaaccgcca
     6721 cggaagaaga tccattctcg tggcgatacg aaatcaaact cggatgactt cacgcacgac
     6781 ggctcgttca gcacacgtca cagtaccgac cccgtaagat aagcctcaag ttgtgcggca
     6841 cacttgggat gacgaagctt ggccaacgcc ctcgcctcga tctgacggat tcgttcgcgg
     6901 ctcacatcga accgcttgcc aatctcttcc agcgtccaat cctgttgctc atcgattccg
     6961 aatcgtaatc gcaacacctg ctgctctctc ggcgtcaacg tcgcaagcat ctgtcgcgtg
     7021 acaactccga gtcggtcccg caccaacgca tctttcggag attcgctccg cgcgtcttgt
     7081 acgaattcaa ccaaaagccg atcgtcctcc gatctaaccg gcgcatcgag gctgaccgga
     7141 tcttcgacga cgctcagagc cttgtgaact tgatcgaggg ggatgccaga gcgttgtgac
     7201 aattcatcag cggttgccac gtaaccatgc tcatgagtta tctgtttttg aagacgtcgg
     7261 agttgagtca aggcttcgaa catgtgaacg ggaacccgga tcgtgcgacc ttgatccgcg
     7321 atggaacgag ccacggcttg ctgaatccac caggatgcat acgtggaaaa acgatagcct
     7381 cgacgataat cgaacttgtc gacagcccgt agcaaaccaa ggtttccctc ttgcacacga
     7441 tcaagaagat ccacgcctct tccagcatat ttcttcgcgg tatgcacaac aagccgcaag
     7501 ctggactcca ccaaagtcac cttggcacgc tgggcgatgg ctcgccctct tgctacctcc
     7561 gccatgatct gctcgaggac ttcttcactc acccccaggg atcgcttccc agcttcaatg
     7621 acctccaacg catggcgaat ggtgtcgctc atcgatgaca actcatccat tctcatgccc
     7681 cgacgtccga gaatgcatcc ctcatcggct tgcgctttcc aggcgcgaac ggtcgctatt
     7741 acctcttggt aaggcatgga agccatcttt tcacacgcac gaataccacg ccttgcatcc
     7801 tcaatctgac gttgcaccgt ggtgaccttg tgatccagac gatcaatttg ttgccgatcc
     7861 catcggatgt cttcaaatat ggccaccatc tggtcgtccc aggttgacgg catcccgctg
     7921 gatcccacgg tgcgatgggc tttcaagcct tgttcacgtg ctttgtggag tgcggaaaac
     7981 gccttgagaa cgctcatcaa gtgctttcga gccgcctcgt cattgtcatc gccatcacgc
     8041 aacacacgac tgagtcgaac ccttccccct tccagtcgtt caagaaggcg agccaactcc
     8101 tgccccacca gcctcgtgcg aagcaaagca tgaaacatga cccgctcgcc gttttccatc
     8161 tgctgagcca acgccacctc tgcctcacgg ctcaacaacg gacgatcccc aagaacttga
     8221 aaccacgcat cgaacgcggt cgatacgcaa gcttcatggt tcgtggatcg atggtcggca
     8281 ggtgcttgac atggaagatt cgatatggac tgcataagaa acccacgtga ttaaggcacc
     8341 cattccagga tgcattcggc gtggatgtca ctgcatgggt catgccatgc agtgaactgt
     8401 cccacacccc cgcgcgcccg agaattcgtg ggcaggcttc gtccttatcc cctatttacg
     8461 aggaaaaatg cctaggtcgg ggagtcccca gttcccagac ggatccgtca gatccttggc
     8521 taatacgact gcaataactg agaagccaac ttccgaatcg ctggctcttg gtcggaagcc
     8581 gccgcttcac ggacaatggg ctgggcttgg gaatgggcga tacgaacaag ggctgtgaga
     8641 gaagcttccc gaaccaaggc aaaagaatcc tgcctggctg cgcgagatag cgcctgaaca
     8701 gcctgagaag aagtattttc aggattgagc aagccgagag cacgggccgc gcggagccgc
     8761 aaagaccatg aagaaccctc caacaacgtc ttgcacaatg cctgtatccc ttcatccgca
     8821 ccctgctctc ccaacgcggt caaggcagcg cgctgaacga gttcgttgtc atcttcaagc
     8881 gcatcgatga ccgccgcgcg cgactgggca gtgttctgac gcgccaacac gcgaatggtt
     8941 cgcgtgcgca agtccaccga aggatgccga acaagagata gaaatgccgg ctccacgcgt
     9001 gcggtaaccc gttgcgccgc cgctcgtacc ctctttcgaa aatccggatc gagcttgtcc
     9061 acaccatcgg taaacggcgc caaagcgtaa tccccatcgt acgacaacaa cgcatctgcc
     9121 acgagacgag caccttccgc cgatgatcgt actgcttgca aggcagaggc gcccacttgc
     9181 tcttcgagtt ccaccaacac ccgagcatag tccgaacggt catagccaga gggcatgaga
     9241 tgatcgagca gcgcatgcac gtcgagctgt ccggccggta cttcccaagg gtcgatggag
     9301 cgcttgaact gaccggtcgc cacgacgaga gctgctgcca aagtcgcttt gcgtgttgcc
     9361 acgttgggat cgaaaaaacc cgtgagcaga ggtcgagcgc gctcgggcgc aagacgcgcc
     9421 aacgccacca aggcggcttg ttgaggcagc gggtcggagc ttcgatagac agccacgagc
     9481 gccgaagctc gatccgtggc tccagcttcg gaaaggccga gcgcagcagc agcgattgcc
     9541 acgtttcccg cttccacgga acgagtcgtc tccgcaagca atgccgcgga ttgcgagccc
     9601 ttgtcgaaac ccagaccgag caaagccaaa gcacggattt ccggagaacc ttgcttggcc
     9661 aattgccgaa gaacaggacg tgcgccagga tcaccgcttc tcgcaacgcc ccacgtggcc
     9721 gccaacgcaa tcgatcctcc cagcaacacg gaagaatcgt ccgtgggcga cagaatggaa
     9781 gcatatcgtg gcagaagtcc gggatctcgc aacgcgccgc aggcaagcat ggctcgggag
     9841 cgcaattccg gttccgctga ccccgtggcg aatgcaaata acgctggagc agcactacgg
     9901 ttctgcacga aagccaacac ctcgatggct atgcgttgct gcgcttcgtt ttcatccgca
     9961 agcgcatcga gcaatggttt gacggcccgc gcgccgatct tactcaattg ctcacgcgct
    10021 gccaaagacg atgccttgga cccatatcgc acgtcttgca ccaaaggaaa cgtcatcact
    10081 ccatacaaat ccaccagcaa acgccgataa acacgcttct gcgggtttcc caaggcggta
    10141 ggcaacaact cacgttccaa cacttcgaga ctgtttttcc caagattgat ctgcatcgac
    10201 agacgagccg ctcttgccac catttcttcg tcgggacata accgcaccac acgacgataa
    10261 agctggtccg cttcgtcggt ttgtcccttg gacaagagca attccgcaag gtcgaagtag
    10321 acgaggaaaa gccgatcgtt tttggtgatg gccgcacgaa actcagcgac cgcttgttcc
    10381 gtgtcttgtc gcttgcgata catctcccca agctttcgat ggccttccgc atcatcgggc
    10441 gccaacgcta ccgcttgggc agcatacttg accgcatcgt cgtcgcggta caattccgcg
    10501 gcgtattggg ccatccgctg ataatactgg cgcgctcgtt tgggatcgat gctggtgagc
    10561 ttggcaagca catcgatcgc ttcatcgatc ttacgctgca acacatatac ccgctcgagg
    10621 gtgagaaata gatcggcttc cccaggacga agctgcgtga gacgcgacaa ggtctgctcg
    10681 gcttcggcaa gctgacgcaa acggatttgg acctccacca acatacgtcc cgcttgaata
    10741 tccggtggat ttgccgaaaa tttcgcggat aacggagaga cttgcgcgcc cagttgatcc
    10801 gacaacgccc aaagagaaac gatatgcacg cgcgcttcac gagcttgcgc ctcgtcctga
    10861 gcgcgatacg ccatatcgag gatcttcccc cagatttgaa gggcttccgc atgcgctccc
    10921 gtcaacacac gacgtcgcgt catcgtcgag ccagtccgtt cgagcgcgat cgcaagggtt
    10981 ttcaaaaagc gaagctgatt cggctccaaa gagaccgctt cccgtagcgc ctccaacgcc
    11041 tcgttcgtca tgtcgtggtc gagataaacc tcacccaagg tggctagcgc ttccgcgcgg
    11101 ttggtgatga ccgtgcgaag acgcgcccat gtttccatgg cctttttctt gtcgccttgc
    11161 tgaaaatacc gatcgcccag atcgatgata tatcgagggt ccctgcctcc gccttgtgcg
    11221 agccgctgca gcaagcgtgt cgcacggtcc ttctcctcga tacgttcata aaagtcagcg
    11281 agacgaccta gctggtcctc gtcgcgaatc tgttgctcca aacgagtcag caaggcgagt
    11341 gctttggccc gatcgcccct ttgaataagg atttcacata gttccagaga aaagtccggg
    11401 ttgccaggca ccgcacgaat gagagcttcg aactgacgaa tggcttgatc gagttcccct
    11461 tgggcctgca acaaatggat gatcttgagg tgcgtgtcga tgtgtcgcgg gttcaaatgc
    11521 aaggcgcgtc gatatacgtc tagcgcacga tcgacttgcc ccgtctcttc cagaagcaaa
    11581 ccgagagtaa cgacttcatc gaagtcgtgg gtccgtgacg attcgagcag gacaatcaac
    11641 tcagggagtt ttccttccgc acgatacgct tcggtaatga gcgcatggat ctcacgacga
    11701 acgcctgcgc cctggcccgc gacttggagc gctcttcgga gaacctcgat cgcttccgct
    11761 gtttttcgtt gtcgaacgag aacttggccc aaatcccgta acgccggtgc cagcgctcga
    11821 ttgtcaccgg cagccgcttt gacgagctgg cgatattcag cttcagccaa ggcaaattca
    11881 tcccgttgca aatactcccg cccgagttcc gcacgaacga aaatggagcc ttgggcaaga
    11941 ctgacaagct gttcgtggta gcgcttggcc gccgtatggt ctttctgatc gagactgatg
    12001 cgaatcaacg tccgaagcgc ggcttcctta tccactcgct cggtgctatt cgccaatacc
    12061 tgatcgaaca aacctcgcgc ttcggccaaa tccccatcgt cgagtaccgc ttccgccaag
    12121 gcacgaaccg ctcccaaagc attgggccgc tgctgaaccg cttcacgata cagccggatc
    12181 gcttccgcct tcttgttgtc aagccggtat accccagcta acgcaacccg tgccgaccaa
    12241 gcttccgtac cgcccgccgc tacccgttgc tccaactccg tgatgagttg cgcaagattc
    12301 ccgtcccgtt gacgatacaa atccgccaaa cgacgcaacg gaaaaacctc gtccggacga
    12361 gccatcacga tggcgaagta ccgcttgatc aaggcatcgg ttcccacccc cgcgtccgac
    12421 gaagcccgag gcgtcgaaga cgccggacgt tgggtaggcg ggccggggcg aggagttcgt
    12481 cgtccctcgg gattgaagtc tgacggtaaa aaagcgtgag ccgtcgagcc atacgccatg
    12541 accacgagga atacatgccc gatgatcgtt ttcgttcgtt tcagaatgct cacggtttgg
    12601 caagtctact ctgacaagct gcctcgggcc atcctccgcc aaagcgaacg ccatttttgt
    12661 tcttgcgcaa aggctatcga atgctcaacc atccggtgcg tgcatggaat gcgtccgaaa
    12721 tgactcgcct cgtcgagtcg tcgataagga gttttgagga aatgaagcgc aatttagtag
    12781 ctttctcatc cgtggtagcg cttggtttgg cgatggccgc ctgtggctat tcagaagatg
    12841 aatggcaggc gcaacttgcg aaatacaatg ccgaagtggc gaaaaataag gccgccaacc
    12901 aacgggtagc cgatctggag caacaactcg aagcctcgaa aaaccgcgtc gccggactcg
    12961 aaatgcaact cgaggccatg gggttggaca tcaacaagtt gaataccgct ttggcggatc
    13021 gaggccaggc tctcgcggac ctcaacgccg acatggacaa aatgcgcaaa gcgctgaccg
    13081 aataccaagc cagggcggca accctggaac gaatcaaaga gcgtatgctc accttgcgaa
    13141 aaaaactcga gtcactcact cgcctcggcc tcaacgtcaa cattcgaaaa aatcgaatga
    13201 tcatctccct tcccggcgac atcctgtttg attcgggcaa aaccgaactc aaagaagaag
    13261 gaaaaaacgt gctgcgtgaa gtcgccaaag tcgtccgaaa tgaccaaaca ttgctcgctc
    13321 gcgacttcca ggtcgcagga catacagaca acaaacccct caaaggagga ccctatctcg
    13381 ataactgggg gctgagcttg atgcgagcgc gaaccgtgct cgtctttctc gttaccccaa
    13441 ccgatatcga accctccggc tacggcgcca accgaatcga acccggcggc ggtttgccca
    13501 catcccgctg gagcgccgca ggatacggcg aaaccgaccc cgtcgccgcc aacgactccc
    13561 cagacaacat gcaaaaaaat cgtcgcgtcg aactcatcgt catgcccaat gtcgaggaaa
    13621 tgctcgatct aaagtcactc acggccgtgc cttgacggtt ctgtccaacc ctctttcctg
    13681 gctccccaca aacggatgca tttcccccga gatggtccta caatcaccac gtgttcgata
    13741 ggacggaccg tcagttcttc gctgggcaag atgttcaagg ggttgtcacg cccctgcagc
    13801 gtgtcctcca ttcccaaggt atccccctgc aacaaaccgg cgccacctcc tggtccgggc
    13861 gtggcaccgt gccgtcctgg ggactcgtgc caaaaatcca tatcaacgca catccctcgc
    13921 aacaaggatt tttccttgat attcgcgtat ttgcggatgt cgagggcaac gggatcattg
    13981 ccttcatcct cgcctggttc ttctttttcc ccgtcgctat cgtccttggc ttcgtggctc
    14041 acaacaactt cgtgcaacag cgtgactacc tcattcattc gctgtggtct cctgtcggac
    14101 acctcatcgc tacacccaac tatccacccg tcccgtttgg cgcccctggc ggagcaccgt
    14161 ttccgggacc ttgaccgcaa ctgctcttgc cgtgtctccc actgtcgtcg tcctgattgt
    14221 gttattcgct ctcggaacgg cgttggcgat cttcgttttc acccgaaaac gcagcaccgc
    14281 tgccgatacg cgccatgacg aacctcccaa gtcgctccag ccacaagctg tattgtctgt
    14341 cattccccat gaacaactcg aacaagaagc agaagaaata gcgcccacgg actacgagct
    14401 gcgaatcttc ggcgtcggac gcacggatgt gggattacgg cgaaaactca atgaagatgc
    14461 gtttgtctgc cttccagaac atgagttgta cgcgcttgcc gatggcatgg gagcccatgc
    14521 cgcaggggaa gtagcgagca agctgactct cgaagccatc agtgatgcct tcgagcacaa
    14581 tcggtacctt tccgcggaaa gctcagaaca accttcccac aggaaaatgc gattggttcg
    14641 aatcctcgaa cgcgccaatc aaatcgtctg gaccatgtcc aacgaagtac aagcgtaccg
    14701 tggcatggga accacggtcg tgatggctca tttctccgtc cacaaacgct atgtgttcat
    14761 cgccaacgtc ggagatagcc gctgctatcg cattcgggac gggcaactca cgcaacttac
    14821 cacggaccat accttgggca gcgtgggcgt cttgggaaaa acaagctccc tgctcagccg
    14881 cgccgtcgga atcgagccga atgtcgaggt cgatatcatg acctatcgaa cgaaacccga
    14941 cgatattttg ctcctatgtt ccgatggcct gagccgaatg gtcgccgatg accagatatg
    15001 cgctaccgtg atggatcatc ctgacttgaa cgtcgcggcc gatgccttga tcgccaaggc
    15061 caaggatgca gggggcaagg acaatatcac cgccgtgttg atccgcatgg aaaaactacc
    15121 cccttcgaac cacgaaggag actcgggatt gcgtgatcga acgacgctcc ctgtttgacc
    15181 gtacgacttg ttggagaatc aaacgcttga taccaccgtc gtgaagcgtt tttcggtaca
    15241 cttctccagc catgtcccat ccttccgccc atcaggagcc tcccaacatt tcaggcgtgt
    15301 tgggatcgtt ggccttgtct gccatacgcc caggttcgtc gacactggcg cttcgtgcat
    15361 tgcgatggtt ccgtgacttg gaacgccttg ggctccgttt gccgttcttt gtcattcatg
    15421 acatgggatt gttgttcgcc gcgggaagcg aacattatgc catcggccca cgatgtgccc
    15481 cccaaatgct gctgggcata ttgtccgaac gctatgattt gtccaatacg tacgtttcct
    15541 tgatccgcga aatggcgact tgcgaggcag ctcgacgtgc cgcttctctg catctttcgg
    15601 atgatatggt tagcgtcctg ctgtcgaaac tactcttttc gttgtcggca aacccgtcgt
    15661 tcaaaccaaa atacccaacg gatctcgtgc ccgacgccag tatgttcgaa aggatcgagg
    15721 atggtcttgt ggagttgtat cgacgattcg atcggtcttt cgagttgcag acgttgcatc
    15781 gattgacgaa ttcgcgattg ctgttgctga ccattgccga cgcgctggat ttggatacgt
    15841 tgcaattgct tggcgtgttg ggagcagagg caggaaccga agcaggactt caagtcgact
    15901 tgttgtccgc catggctaca cccgcagctc acgatgtcgt caacttctcc cttgatatcc
    15961 tgccaagtgt cttggagtcc aaaacccgtc caggcgcctc agaacgcgct ggattcggct
    16021 gcggtggcct tggcacccgc ggtagcatcg acaacctcgt tctcaccgag cttgcctggg
    16081 acgccgatga cttcacacga cgtgtcatcg ataacgaagt cctttattac acgaaagaaa
    16141 cagaacgaga acccgcgggc cgtcgtcata tccttctcat cgatgcttcc gcctccatgc
    16201 gcggagagcg ccaagtgttc gcacgaggca tggcacttgc cacagccaaa aaactggtgc
    16261 tcgaaggaga agacgtctcc cttcggtttt tcgacgcacg gttgtacgag ccgcaatcgg
    16321 cgcgagcagg acgtctgcct acaccgttca tcctctcgtt caaaggcgaa cgaggacgca
    16381 atccccaacg cgtgttttcc gacctcatca cggctctttc catcgaaaaa atccgtgacc
    16441 aacgggatgt cgtgctgcac gtttttaccc acgcggcctt gtatatccca cgacgaatcg
    16501 tagaaaacct cgttcgtatc gcacaactcg gcattgtgtt cattctgcct tccggaggct
    16561 cgctcgactt ggattacctc gacttgctcc atgcccactt cgtggtggac catgacaccc
    16621 ttgcctctcg aggagcacgc gccgaaaaag cccgagatat ccttggacaa atcggtagcc
    16681 agtcgcccca atcaccaatg gttgccgtct ctcactcgat gggagaaacg tcatgatagg
    16741 tggagatccc gatgttgccc gtacgatcgc gcaggcccag cacgatatca gagtcggaaa
    16801 tttaagcgca gcccttgaaa tactccttca atcgctcaat caaacgtacg tcacggatca
    16861 ggactacatc ctcgcgctaa gacacctcgt cgatgttttc ggccgcctgg gtgatgcccg
    16921 cagggctatc acatgcgcgt ggtacctgga cgacgatcga ctcgcagagt cattgatgcc
    16981 cagagtaacc gctgaagatc gggctcgaac gtacatcgcg caagcgttgc gatggccatc
    17041 gggaaacagc cgcagagccg aactcttcgc ccacgccgcg cgggagttcg agtccgtagg
    17101 ttgcctcgca caagcagccg tcaacctgga acgagctggt ttgttccgtg acgctcatcc
    17161 cttatggctt cgactctgcc acaccattgc cttgcatgat gaggatgata tctacgccgc
    17221 gggactcgcg tacttcaacc tcgcaagaac tgccaaacgc gatggtaacc agaaagaagc
    17281 gcaggcggcg acggtaacct cggttcattt gttggaagaa gccgcggacc gatttgaaca
    17341 gctaggacaa cgggaacgcg cttttgattg ttaccaagtc ctggtcgcca ttggacgaga
    17401 gacggaaacc atcgagcatg tgctcgaagg atatgtgaac cttacgcgta tccttcgaga
    17461 ggaccatctc cgaacctatg ccttgaaaga ataccaagcc gcgatccagt tcttacgaga
    17521 acgctcggag atcgccgctg ccgccaccct ctgccgggaa atggcagact acgcccgccg
    17581 tcaagggctc cttcccctcg ccaaccgggc aatcatcgac caagcaggca tgtggcgtga
    17641 gcttgcaacg accgtcgttc agcgcggaca ggggcctgaa attgccgaaa acgcactgct
    17701 tgccgcggtc ttgtcctacg ccgaactcgg ccagttcaaa ctcgcgggca atgtttatgt
    17761 ggaactggct caccttcccc tcgagccatc acgacggaac cattatgccc gagcggccca
    17821 acgctatcaa aatgcacagg atacctcgat cgacttgggt caattaccca atgccgtccg
    17881 tggggattcg gaattccccg acgtttggca tgtcgacgtg atcgaatggg agcaaagggg
    17941 agtcgtttcg gaagtgtgtg ccgatgtcat gctcgatcga gccacatggt ccgaagtcat
    18001 ccgacgtcgc gccatggttg cacgaatgac cgcgttgcca tgcgaaatgg ctcctcatgc
    18061 acccatcggg gcgtggcttc aggttaccga aaacctcgct caaaccgagc tgtatgcaac
    18121 catcgcacct ctcgaaaaaa tgtatcgtcg acccgaaccc gaaatccgcg ccgccgttat
    18181 caaagcgctt ggtcgtttcc tattcaaacg atccttcatc acgctgcgac gtgcgttatt
    18241 ggaccctgag cttactgttc gaacccaagc ctggaacgcc attcgccagc ttcgttttcc
    18301 ccatgcattt gaccccctgg cgcggattta tcgcgagtcc accgacctgt ctgcccaaca
    18361 ggccgccctg gctgccctcg ccatggtgga taccgaagag gctgcagaat tcatgctttc
    18421 cgtgcttcaa caagtcggtt cctccgaacg ctccctcatt gactcatgga tcagatcctg
    18481 cgggccaagg ttgatccaag ccgctcgtga tgctctgccc tccgcttcgg gagacttcgc
    18541 cgacctgttg cgcttcctgc tcaactccca aacgagtcgc ttgcgctaac ggatcgtccg
    18601 aggccagatg cctgccgaca atggcctttt tcgaatacgc ctatcgaacc gcatcatccc
    18661 cctttgcact tgccaaacgg cttcgcactc atggcaagac attccttacc ttcaccccga
    18721 acgcatggct tcccaggctg ctcgctagac gtctcttcag gtttccgaat cgtaaaatag
    18781 cttataaaat ccgaatctta cagtctttct catgccatcc gttcgactca tgaacgatgc
    18841 gatagatctc ttcgttcgga tctcctggca acgcctcgat ctcaaaagcc acgatatgta
    18901 gcctgtcgaa acgctcccgc aactcacacc gaaaaccgtg agcttccaat acctcggcga
    18961 tctgcttctg cctccttcgt gaagcataga gcggatcgat cgtgaccaca cctttgatca
    19021 tctcgtctgt cactccttga actgttttat tctcagtctc tcacgacctc gttacgggga
    19081 gccagtcttt cgatgagcaa cgctgaacag ttcttccgga tgctgatgac aggctcgccg
    19141 tgacagacgc tcgggccaga actcgattcg cttgcaaacg ccgcacaaaa ctaagtcatg
    19201 ctcatagtcc tgccggtgat cgagaaaatc cgcggacagc agggcaagaa tttgatcgct
    19261 cagcgaatga cgttccgtca gcatcacttc ccaactccgc tgcccgctgg gttgacgacg
    19321 ccgaaccacc cgaccgtcgt agatggccgc ttgcacgaac ctgtcgtctg gtgtgccatc
    19381 atgcaatccc gacagcgtgt cgaagactcg ggcccgcgtc gcatcgatga gcgtcgatgc
    19441 cttgccatgg ggcaaataca acgctggatc cgcgtggcca ctgagattac cataactcaa
    19501 ttccccaagt ccgcgctccc acaccgtcac ccgaggaaaa gggtggcgcg tcagcaatcg
    19561 gcccggacga atcaaacact gatccatcca cgtcaccaag tggtggattc cccatcgctc
    19621 gagcgcccca tccaaaaacg ccaacgccga ggtccgaaac gataccggtg atcgtcgctc
    19681 actatccggg ctctgcccac gaggattgcc acgcctgctc tgcggcaagt cccgtgaaaa
    19741 gcgatactct gaatcgttcg gactcatcct tccatagcgt atggaaatct gtatccgcga
    19801 cgtcaatcgg ccccatgcac ggattcgtca tgatccatcc gaaaagatgc atggcgcgga
    19861 acggcaccgc gggttgcctc ctgctccact tccggaatca atcccatctg caacatcgac
    19921 acatgacccc cttgtgccac gatcacatga tccagaagcg ggatcccaat acataacgcc
    19981 gctaccgcca gcttttccgt caacgttacg tcctccacac tcggtcttgg atcccctcca
    20041 ggatgattgt gcgcaagaac gaaacattgc gcccccgcct gcacggccac acgcaatacg
    20101 tcgcgagcga gaagcgcgca gccatgctgc ccccctttcg ctacttgcca tcgggacgtc
    20161 accacgtttc gcgcattcat gcccaacaac cacacctgct cgtgctgcaa aagccggaaa
    20221 cgaggaccca gtatcgccac gatatcttcc gcgcaagtaa ccaccgacgt gggcgtgaga
    20281 ctcggctccg cacagcgtaa tccaagctcg agagccgccg ccaaacgata aacctgattc
    20341 gcggtcactc ccgtcattgg ggcataacaa ggcccgatcc gcgccaaacc cgccaatccc
    20401 cccacctgct tcaacaaagt agccgcccgt tggcgcgaac ccccccccgg gcgcaaagca
    20461 agcaagcagg ccaccaattc ctcgtccgat accagactcg gatgaaatgg cgacgttgac
    20521 caagaaacgt gatgattggt ttttctacag cgcattccct ggtgcaatgc atcccccggg
    20581 ccaacgtaat ccgttgattt ttcaagagat tggctgctga cacccctgac gctccgccac
    20641 cccatggacc gccttttctg cgctgggcta taaaaattag tttatatttc aatggattat
    20701 ctatgactgg cggccgccac cccgaaaagc ggcggtcgcc acccataccc ccagccgtct
    20761 cgctgcttct tccccgcagc cataccatcc cgccgcatag actcacagca cccgcctttc
    20821 gacggccacg acgaagaaaa cgaaagtaag cgcttctttc atcttacaag tttcaataat
    20881 ttatgaaagt tttggcgcat ggcgacctcc ttcgatccca ccacggccat cgaacacctc
    20941 tcctcgatct ccagacgcaa tcccggagcg aagcacgcga cacctcgcct cgacgatgtg
    21001 acacgactcg ttcatggcga actggcatcg gtcgagttgt ggttgaaaga cgccatggcg
    21061 cgggagtcga gttgctccat cgccgcatcg gacttaattc gcgccggcgg aaaaagaatg
    21121 cgcccctcga tcgttctgtt ggctacccga
//
LOCUS       DAOSOR010000028        20411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_2615_length_20411_cov_4.150079, whole genome shotgun sequence.
ACCESSION   DAOSOR010000028 DAOSOR010000000
VERSION     DAOSOR010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 20411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20411
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2615_length_20411_cov_4.150079"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PK710_02735"
     CDS             <1..280
                     /locus_tag="PK710_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08654.1"
                     /translation="VDLWAVGVMLYECIVGKRPFLGRNFPELASRIAYDAHRPMWERR
                     PATPELLQQVVDRALAKAPENRYQKASTFLRDLQVVRMALLNENRSSP"
     gene            382..1242
                     /locus_tag="PK710_02740"
     CDS             382..1242
                     /locus_tag="PK710_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT08655.1"
                     /translation="MVSRGHPHSFRPALRTGKKYDASASDRPILVFEDVYKAYRADQP
                     VLRGTHLVIERGEFVFITGPSGSGKSTLLRLLYRAETVDGGRILFLGRDIARLTHDSV
                     PMLRRNIGVVFQDFKLVPTWSVFDNVAVALEVLGLPARIIHTRVGEALDRVGLAGRGP
                     DRAGVLSGGEQQRVAIARAIVGEPALILADEPTGNLDPLLATDILGLFEDIHATGTTV
                     LFATHDRTLLDVRPRRVVVLDDGRAVDVPNGLGVEPQADDFGTAAQEDTASEGYELPV
                     VTPTDRLAVA"
     gene            1271..2149
                     /locus_tag="PK710_02745"
     CDS             1271..2149
                     /locus_tag="PK710_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOT08656.1"
                     /translation="MGSMSTASRAWRGGRADWKLHVLSGFSLAVAFICLASALLVVFN
                     LDSVRQRWSRAGRASIYLRDGATPPMVADLRTALDKTPGITKVRFISQQEAKQEVVGS
                     SKDASLAAIPPEAFPASLEIDVASSVSAVDLAGITTSLKQIPAVESVETYEQYTTKLQ
                     GLVQAGVIASLILSLVVLAAVVSVVGSTVRLALQRRRVEVDVLRLVGATESYVCRPFV
                     LEGSVLGAAGAGVAVALLGLLYLILRAHLAEVVSIMVGVEPSFLPWYAALGLVGFGAI
                     LGAVSSHISLRRMVVL"
     gene            2176..3162
                     /locus_tag="PK710_02750"
     CDS             2176..3162
                     /locus_tag="PK710_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOT08657.1"
                     /translation="MRSQSLTVFPALLLLSGLALAQADYAATMPAAGPAALTQDVDAE
                     LAQVNHELQQLERHIDALRVQSDVVRQRTMARARTYYHLTHAGLLPIGTGFEGLLDHT
                     SRVERLRRAIRKDVDSAQALNQQQAKLLSMRKNLLDRQQPLELKRRAMTEAHAALMEA
                     SDRKRAFERAFGSPAEGEYTAVYGAPADPSFGAGDALRGSMGGFRSLEGRLPFPIAGR
                     TEVRLVRRPGAGGPGVEMMAPVGTAVLAVHSGRVAFADEVDPYGRVVILDHGEQYFTL
                     SGDLGTIDVRVGTDVGAGTRIGTVGRSKGQGILYFELRQGADTLDPGPWFGL"
     gene            3320..4048
                     /locus_tag="PK710_02755"
     CDS             3320..4048
                     /locus_tag="PK710_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOT08658.1"
                     /translation="MSIYRDDLAAARERIEALETELRHLQRKNEVPWRSDDSLVQPRS
                     SFGRTALALGGVLLLGLLALRITAIGPLAAGSLLLLLLILSLRGRTLVVRPHEVAMVT
                     VKQGQRYSWVAPGRTAQLRSGSVAESFSLRAIPIFFEMRHLPAVDTALRVEVFAVVGG
                     RLDEEGIERAVQIAATTPMEPVVRAALQEVVREVVKDASYSDLAARTGSLRERLRREC
                     RESLHRFGVEVLVVGWLDVREEST"
     gene            4045..4608
                     /locus_tag="PK710_02760"
     CDS             4045..4608
                     /locus_tag="PK710_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08659.1"
                     /translation="MILVLEGALFLAIVVAMVAMLFRLPCPKDTALILTLRSNGLPLR
                     SGEPMFRVLVGKAGFRSPAVAAVDSLDLRAFPVEALLMIDEHEWLVTMRAHVGGQESM
                     VRRAAVRLLGMSQRDREAIGEGVLALAVRDALVGRGGGEVVEEQREAIEKDVRSRVAA
                     VLEPMGLEVLELSLTIRRREKSDTGPR"
     gene            complement(4680..5897)
                     /gene="sat"
                     /locus_tag="PK710_02765"
     CDS             complement(4680..5897)
                     /gene="sat"
                     /locus_tag="PK710_02765"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HOT08660.1"
                     /translation="MKTLKESRMIKPHGSDKLNPLFVMDAKERDALAKEAESLPSIVV
                     CSAAAANAVMLGSGYFNPLTGFMNKADTLSVAEGMKTASGVFFPVPVVNMIQDASAIK
                     GAKRIALRDPNVEGNPVLAVMDVKAIEELTDDELKMITEKVYRTLDPEHPGVAAFTTV
                     GKVLVSGPIKVLNFSYFDVEFPETFRTAYQIRDEMQKLGWNKVVAFQTRNPMHRAHEE
                     LCRMAHADVKADGILIHMLLGKLKKGDIPADVRDAAIRKMVELYFPPNTVLITGYGYD
                     MLYAGPREAVLHAVFRQNCGCTHLIVGRDHAGVGSYYGPFDAQTIFDDIPKGSLEIEI
                     YRADHTAFSKKLGKVIMMKDAPPGHTKDDFVLLSGTKVRTMLAAGEALPPEFARPEVA
                     EILMKYYQSEEGK"
     gene            complement(6198..7613)
                     /locus_tag="PK710_02770"
     CDS             complement(6198..7613)
                     /locus_tag="PK710_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOT08661.1"
                     /translation="MVHDSPNFPPSPLSETEVPPGATTILVVDDEASHLESIEKILQR
                     EGMRALTAPNGRTALELARRHRIHVVLTDLMMPGTSGIELLRAVKEISPDTEVVVMTA
                     YGTVEAAVQAMREGAYDFIEKPLKRVTLVKSLKKAAERWLLLTENRTLKRELKRLRHR
                     EIVGNSHAMRRVLDIATQAAPSMATVLVLGESGTGKELIARFIHDQSARPTGPFVAVN
                     CAAIPESILEAELFGHEKGAFTGAIARREGRFAKAAGGTLFLDEIAELSASVQVKLLR
                     VLQEGEFEPIGGDTQRADVRIVAATNRDLRAEVAAGRFREDLFYRLNVIAITAPPLRT
                     RREDIPLLVDHFLGVYCAKNGRPRLETSRVLLQALMDYDWPGNVRELENAIERAVVLC
                     KTTVLTEEDLPDVIAQGSNLTPNVLSIPVGTSLDEVERKLIRETLRHTRGDKSLAAQL
                     LGISTRTIYRKLDEVGWEETD"
     gene            7772..8773
                     /locus_tag="PK710_02775"
     CDS             7772..8773
                     /locus_tag="PK710_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT08662.1"
                     /translation="MDQFSAHLDRGWDLVQKGDTRGAEASARQAIELDPNSPEAYNLL
                     GFVAALEGDADEAIEYYSQAIALDETYLEAMLNAAEVYIHPLAQYDDAISICEQALNL
                     TESDEEEIDTLLLMFDAMLGKGDVVSARTVVLRIPTGPYENPNHTFLVGRALFEIGEI
                     TRAAPLIEEAANKDPMQSEAWYYLGLIRDERGDPRGATEAFLRAREVDLREPPLPWSS
                     PRDHFMLAVTKALQALAPMLRAFVDQAEVFLSEVPGIELVADGVDPRALLLLDALATP
                     ELPGPPCGRIFVYQRNVERVAGSLDRLEAILVEAFEREIQAVFLESTSEPPPAAVLN"
     gene            8877..9554
                     /locus_tag="PK710_02780"
     CDS             8877..9554
                     /locus_tag="PK710_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT08663.1"
                     /translation="MRWGSENERSLPQTSKVTAVTPSDAADLLDEPRLVQRAKAGDRR
                     ALGTLLSHHGPRLYRAVLLPRLGNEARARDALSITYEKAIQHMPQYEWQPCGIYPWLR
                     VIALRTALDMLRARRREVLFDPQDVSRELESAQASVSSSMPGLDAFHERDELDAVRAM
                     LHDALARIHPRYATAIRLRILEDLPREQVAEQMGVTPATFDVLLHRSIASLKKVMDVR
                     GTEGSEP"
     gene            9551..10033
                     /locus_tag="PK710_02785"
     CDS             9551..10033
                     /locus_tag="PK710_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08664.1"
                     /translation="MTVYDDKGWEELTPPTPQEVEQAQALREALEAGEDHPDADLLRS
                     LRFLENPPPLEPRVNEWLVHRALHKMAPSHRGRWVASGVAAALALAAGVVVVLSTGPD
                     PTTAPVVRASLVSCRSTQSLFHEPFDRHGGTSSRMDRIARDRARDYRTNLFLRGGVQP
                     "
     gene            10030..>10345
                     /locus_tag="PK710_02790"
     CDS             10030..>10345
                     /locus_tag="PK710_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08665.1"
                     /translation="MTTKLIRLVFLLGLLWGMGCSQRSDTPCVCEATPVVDTALMAFL
                     SKAKAAHHQADLLEQSGQVDAAIEVLDKVTSFTQMSDRAEVREVLADTLARLSDLRGQ
                     RGQ"
     assembly_gap    10346..10355
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10356..10606
                     /locus_tag="PK710_02795"
     CDS             <10356..10606
                     /locus_tag="PK710_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08666.1"
                     /translation="WEESEADIRKGLELAPSGSYFEGHLYEMRGVNEERRAKAQAEQG
                     DAEGAQTARRRAIEAFERAIQIQDSVIRRELSDEGADR"
     gene            10603..11124
                     /locus_tag="PK710_02800"
     CDS             10603..11124
                     /locus_tag="PK710_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08667.1"
                     /translation="MKTLSILRAGCISLLLMGCARAPVYPASPSAPESSGQAVQEVSR
                     PESTAEFEPPAEQSRAPSEPSSLPVAPSTKRNYALPPDSYDGGHEGVRSLWEELSAAE
                     QVALSVRSGCERACRALLSMHRATERLCELASDEPEKKACQQARQRLREAKETVRRSC
                     QRCEQGPSLTSDD"
     gene            11191..12123
                     /locus_tag="PK710_02805"
     CDS             11191..12123
                     /locus_tag="PK710_02805"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT08668.1"
                     /translation="MGDLRLGQSWTWAQRWKNEFLFHAATLGLWWGRRLSEPARQALG
                     RRLGRVGARLWTAGMRRAGHRLRQAFGEAMPVTPIEVFETLGADYADTLSLMHRSFRA
                     AERMPLASGAKRALDEAVERGKGVIFLTAHFGPMDMMAASVAERGYRVATLARESYDP
                     RFTAFFEALREPRGVRAIYRGRAGAEMAILRALRDGYLIGFPIDFAGRGMRTREVSFL
                     GQTVPMAVGPAALALRFGIPVVVGAPISVAGGLQVFVEPFMELHHGDDGATVGAMARA
                     LEQRIRDLPAHWPWMHDTTGVPRSRALPDVKRCQ"
     gene            12228..13784
                     /locus_tag="PK710_02810"
     CDS             12228..13784
                     /locus_tag="PK710_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOT08669.1"
                     /translation="MTLPLTPAEAFLLSRIDGHQTARDLAMMTGIDEAMLMESLDRLA
                     QMGAVTWDGAEPAERKEREKKSDAERVVRSIPSPVVEERVVDPRPEPLPLYDPSELDE
                     PCDLPEDKRRTILDAFYRLEDMDYYELLNVPSTATKKEIKDSYYRLVAEYHPDQYFRK
                     NLGSFKQKMETVFSRLTQAHDTLTRKQSRAEYDSYLSTVQSTRRLEESLRSSRATVDD
                     SSTGEARTRDERVEPSSVLGAGRREMETAPVRRGETEEVKREETVLQEPLVSGQPMRS
                     SARPAPTRESMRARREAFAARMSGGRVSRIPPPPTPSSEPPARPSVPQGVAAGEALLR
                     HVTERKEAVRQAQIDKYLQAAKTALEQGDFAAAANSYRLALHMEPDSAEIQEAFETTQ
                     ARATVELAGSYLQQARYEERAERWLEAARSYSRAADGMLSDAQVQSKAAEAMWKANLD
                     LRKAAEYAKRALALDSNNPEYHVILGKIYLAAGLVLNARRELELANELSPNDATIAKL
                     LEAVRKGGKA"
     gene            13801..15627
                     /gene="dnaK"
                     /locus_tag="PK710_02815"
     CDS             13801..15627
                     /gene="dnaK"
                     /locus_tag="PK710_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOT08670.1"
                     /translation="MDRVIGIDLGTTNSCVAIVDDGTPTVIPNRGGYKTTPSMVAVTE
                     AGKRLVGHIAKRQAITNAENTVSAAKRLIGRKWNSSQVKNSVLTSSYQIVEGPHADVR
                     IKLRDNSYSVPEISAMVLQEMRVIAEDFLSESVSKAVITVPAYFNDNQRQAVRDAGQI
                     AGLDVIRIINEPTAAALAYGFGKNYEKLIAVYDLGGGTFDISILEIGAHGVFKVIATA
                     GDTFLGGEDFDALIIDWLVQSFKEQHDIDLRQDRMALQRLKDAAEKAKCELSSVKETE
                     INLPFIISSGRNEALHLQQTISRQTLETLCEDLVQRTVEICRQTMLDARLDKDEIEDV
                     ILVGGMTRMPRVQEAVAGFFQREPCKGVHPDEVVALGAAIQGAALVDDAHEMILLDVT
                     PHSLGIMTFGSVFEELIAQNTTVPTAQTKIFTTSRDNQTAVKILVMQKEGQQADDFEL
                     LGEFILTNLRRAPKGQVEIEVTFEINADGIVNVSAKDMETGQGQSIQVTASSGLTKDE
                     ISNMIESAKEYMVERRSSEEFEAARQEAETLIAEIERLFPQVEVVVAASDFGREAVNK
                     ARRILKDAKSAIESRDLNQVKSQTEALARTHRMFKGVVTKTQ"
     gene            15633..16796
                     /locus_tag="PK710_02820"
     CDS             15633..16796
                     /locus_tag="PK710_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08671.1"
                     /translation="MAKKPDTTSIPPDTGEIDTDWSEASLQQSAEGSGIPTAVLPEPY
                     ERYAKQVTSQPMGEETRKALSAPRLPAIDESVLDEFLEQHSRDSGEMRAMGLVGRQLS
                     GPYAIPLELNDEGEPVRASERAMESPSELLSDSGAVRTTPVPSSKDWTAESPTPHKPV
                     PEVLEAFRRASALEPAGSDNDLVGREEYFELDEPGEIGMEDSLEINLDEVVPLPFDLS
                     QALQVDRKPTPRPEPIDPIEFPLENDGGALDLVSARATPFVPQPTDPIIDLRDRFAVG
                     DFTGAHAVAEAILAEQPNNTEALRFRESCRDVLTQMYTARLGPLSGIPKLAIPVVELQ
                     WLSLDHRTGFLLSCVDGQSTIEEILDVSGMPTLDALRILFTLLQQQVIEIVNR"
     gene            16815..17102
                     /locus_tag="PK710_02825"
     CDS             16815..17102
                     /locus_tag="PK710_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08672.1"
                     /translation="MPAICTESIRRIARSASLHLENDEVENMARDLSKDLEALQGLRE
                     IDFEGPSLDDEKNVVLRRDTPEQSLSMEQVKSQSERATPEGFRVPPFLPHA"
     gene            17135..18607
                     /locus_tag="PK710_02830"
     CDS             17135..18607
                     /locus_tag="PK710_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOT08673.1"
                     /translation="MKAVQDILNSPVHRLVEQVREGSLTAVDIAKAFVDNARTGDPRI
                     RAFVHFDDASIERQATIIDEKRKRGEPLGALAGIPVAIKDSICTIDAPTTCASRSLMH
                     GSLAWRSPHESTAVARLRKADAIIFGKTNMDELSMGCSTTRSAFFPTRNPICEELVPG
                     GSSGGSAAAVAARMVPLALGSDAGGGIRQPASFTHTVGIKPTYGRVSRFGLVGVASSM
                     EQIGPLARSVQDAVRLLDVVAGRDERDATSMQASTSPLEPYCHQSMDGLRIGVAHEYE
                     QDLPDDILVAVKQSLEALESAGCRLLPIRLPHTHLALSCYHACVAAEASSNLARFDGI
                     RFGATDPGGVDVEQWRGCVRGRGLGWEVKRRVMLGTYLLSAQHREGLFEQARKARCLV
                     AKDFDEAFSHVDAIATATSPMFPWPIEIGDESMHRADAHTVAAGLAGLPALSVPCGVV
                     SQVLPIGLQLIGPAWREDILIRIASRWEQLREPASFEREG"
     gene            18607..19857
                     /locus_tag="PK710_02835"
     CDS             18607..19857
                     /locus_tag="PK710_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08674.1"
                     /translation="MISWRYTFLGVMAVTVLGAMGCSSCRKQQRHDFDSSAPMASFEV
                     APGRLFSPWFWEPTDARDVALPAGCEFVGPPLRHRRSTTNLFFFASPGELHTLAMAQG
                     DPNPDSVTESRGLVTLSDKGAGAVRDVPWLHLTDPPLMTSSSQGWLALLVESDGLGLA
                     TRLWLWREAGAMELVAEGDRLQPIDAQCNQNGCALLTTRVGQVATRGATAWLGQPMAP
                     MATWLRLEIPPDAVADASVPLGLVSWDASSHSALIAFEDSDRISFLRVTDTKVERQGS
                     IERDYGIVDYLPMRDGPMVVTTWGEPDDIGCVPGGAFVQFTTLGKQAHRLRVAMLPSS
                     GYARALEHGALITWSAPMQCNLPNRKVVYAVLLHADGAPASSIMAVGNADGHAVSTRG
                     NEVHLWLSDLVGVTWLRAKCRPPM"
     gene            complement(19869..20228)
                     /locus_tag="PK710_02840"
     CDS             complement(19869..20228)
                     /locus_tag="PK710_02840"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOT08675.1"
                     /translation="MNTDTLPASEITALLGRGTHFEGKLLFDGRLRIDGSFRGQIRSN
                     DTLIIGDGAEVDAEVDVDNVIIRGGVVRGAIRARGSIELYVPAHVTASLHSPSVFIDK
                     GVHFEGTCKMAEVETDS"
BASE COUNT         4005 a         5313 c         6568 g         4515 t
ORIGIN      
        1 ggtggattta tgggccgtcg gcgtgatgct gtacgaatgt atcgttggga aacgcccgtt
       61 tttaggacgt aatttccctg agcttgcgag ccgtatcgcc tacgatgccc atcgacccat
      121 gtgggagcgt cgtccggcta cccctgaatt acttcagcag gtggtggacc gggcgttggc
      181 aaaagcgccg gaaaatcgtt atcaaaaagc ctcgacattt ctgcgggatc tgcaggtggt
      241 gcgcatggca ttgcttaacg aaaaccgatc ctcgccatga accggcagcc ggtgattggc
      301 tgatattccg ctgctttgcc tgggagacgg aagcgttgtc gaaaacttgg tcgtttcctt
      361 cccggctgat aggcatgggg catggtgtcc cgcggtcacc cccattcttt tcgtccagcc
      421 ttacgaacgg gcaaaaagta cgacgccagc gcgtcggacc gtcccatcct ggtcttcgag
      481 gacgtgtaca aagcctatcg tgccgaccaa ccggttcttc ggggaacgca tctggtgatc
      541 gaacggggcg aattcgtgtt catcacggga ccgtccgggt caggaaaaag caccttgctg
      601 cgcttgcttt atcgagcgga aaccgtcgat ggagggcgaa ttctcttttt aggacgcgat
      661 attgcgcggc tcacccacga ctccgttccg atgctgcgac gcaatatcgg tgtggtattc
      721 caagacttca agctcgtgcc tacctggagc gtatttgata atgtggcggt ggcgctcgag
      781 gtgttgggct tgcctgcacg aatcatccat acgcgggtgg gagaagcctt ggacagggta
      841 gggttggcag ggcgcgggcc ggatcgggcg ggggtattgt ctggtggcga gcagcagcgt
      901 gtagccattg ctcgggccat cgtgggcgag ccggcgttga tcctggccga cgagcccacg
      961 ggcaacctcg atccgctgtt ggctaccgat atccttggat tatttgagga tattcatgca
     1021 acgggcacca ccgtgctctt tgccacccat gacaggacgt tattggatgt tcggccacga
     1081 cgggtggtgg ttttggacga cggtcgtgcg gtcgatgtgc cgaacggtct gggggtggag
     1141 ccgcaggccg acgatttcgg cacggcggcg caagaagata cggcgtcgga gggatacgag
     1201 cttccggtgg tgacacccac cgatcggctg gcggtggcgt agtacgcgct tgggcgcgag
     1261 aaggatgacg atgggttcca tgtcgacggc gagcagagca tggcgaggcg gacgggctga
     1321 ttggaaactg cacgtattgt ccggtttctc gttggcggtg gcgttcattt gcctggcttc
     1381 cgcgcttctg gtcgtattca atctggattc cgtgcggcag cgatggtccc gcgctggacg
     1441 agcttctatt taccttcggg atggggccac gcctccgatg gtggcggacc tgcgcacagc
     1501 cctcgacaaa acgccgggaa tcaccaaagt ccgtttcatt tcccaacaag aagccaaaca
     1561 ggaagtggtc ggttccagca aagacgcttc tctagcagcc attcctcccg aagctttccc
     1621 tgcttctttg gaaatcgatg tggctagctc cgtttccgcg gtggacctgg cgggaattac
     1681 caccagtctc aaacaaatcc ccgccgtgga atccgtcgaa acctatgaac aatacaccac
     1741 caaactccaa ggactcgtac aagcaggagt cattgcctcg ctcatcttgt ccttggtcgt
     1801 gctggccgcc gtcgtttccg tagtcggttc caccgtccgt ctcgccctgc aacgccgtcg
     1861 tgtcgaagtc gatgtcttac ggctcgtagg agccaccgaa tcgtatgtat gccgaccgtt
     1921 cgtgctcgaa ggaagtgtat tgggagctgc gggcgctggg gtcgccgtgg cgctgctggg
     1981 gcttttgtac ttgatccttc gcgcccatct ggcggaagtc gtaagcatca tggtgggcgt
     2041 ggagccgtcg tttcttcctt ggtatgcggc gctgggattg gtgggcttcg gagctatcct
     2101 cggagcggtt tcctcccaca tcagcctccg gcgtatggtg gttttgtagc atggttcaat
     2161 agcgaggcgt cattcatgcg gagccaatcc ctcacggtat tccctgctct tttgctgctt
     2221 tccggcctcg ccttggcgca agccgattac gccgccacga tgcctgctgc tggccccgcg
     2281 gcattgacac aagatgtcga tgccgaattg gcccaagtca atcacgaatt gcagcaactc
     2341 gaacgccaca tcgatgccct gcgggtgcaa agcgatgtgg tgcgtcaacg aacgatggct
     2401 cgggcaagaa cgtactacca cctgacccac gcgggacttc tgccgatcgg taccggattc
     2461 gaaggcttgc tcgaccatac ctccagagtc gagcggcttc gacgagccat tcgcaaagac
     2521 gtggactcgg cccaggcatt gaaccaacag caagccaagc tgctgtcgat gcgaaaaaat
     2581 ctgctcgacc ggcaacagcc cctcgaactg aaacgtcgtg cgatgaccga agcccacgcc
     2641 gcgctgatgg aggcttccga ccgtaaacgt gcgttcgaac gagcgtttgg ttcacccgca
     2701 gaaggtgaat acactgccgt gtacggggcg cccgcggacc cctcgttcgg tgcgggcgat
     2761 gccctgcgag gcagcatggg cggttttcgc tctttggagg gacggttgcc gttccccatc
     2821 gctggacgaa ccgaagtgcg gctggtccga cgacctggcg ccggtggacc cggggtcgaa
     2881 atgatggctc ccgtgggcac cgccgtgcta gccgtgcatt cgggccgagt cgcctttgcc
     2941 gatgaggtcg acccctacgg aagagtagtc attctcgacc atggcgagca gtatttcacc
     3001 ttgtcgggag acctgggaac catcgatgtg cgggttggca ccgacgtggg cgcgggcacc
     3061 cgaatcggca ccgtcggcag gagcaaaggg cagggaatcc tctatttcga gcttagacaa
     3121 ggcgcggata cgctcgatcc agggccctgg ttcggtctct gaactgattt atcttgttga
     3181 ttttgttgat gtttattgaa tctcgcctcc ccattctcca gccgacgtga ggcttgagcc
     3241 tcagcccatg gcttgcagtc ggcgctggtt gccataggcg tttgcctttc gcctcgctat
     3301 catcaccctt gttgctacca tgagcattta tcgcgacgat ctggctgctg ctcgggaacg
     3361 gatcgaagcg ctcgaaacgg agcttcgcca tcttcaacga aaaaacgaag tgccgtggcg
     3421 ttccgatgac tctctcgttc agcctcgatc atcgtttggc cgtacggcgt tggctctggg
     3481 gggggtgctc ctgctagggt tgctggcctt gcggataacg gccatcgggc ccctcgccgc
     3541 ggggtcgctt ttgttgttgc tgttgattct atccttgcgc ggtcgcaccc tggtcgtgcg
     3601 tccccatgag gtagcgatgg tgacggtgaa acaagggcag cgctattcgt gggtagcgcc
     3661 ggggcgaacg gcgcaattgc gttctggttc ggtggcagag tcgttttcgc ttcgtgcgat
     3721 tccgattttt tttgagatgc gccatctgcc agccgtcgat accgcccttc gggtggaggt
     3781 tttcgcggtg gtgggggggc ggctggacga agaagggatc gagcgtgcgg tgcagatagc
     3841 ggcaacgaca ccgatggagc cggtggtgcg agcggctttg caagaggtgg tgcgggaagt
     3901 ggtgaaagac gcgtcgtatt cggatcttgc cgctcgaacc ggaagcctcc gtgaacgatt
     3961 gcgacgcgaa tgccgtgagt cgttgcaccg ctttggggtc gaggtgctcg tggtgggttg
     4021 gctcgacgtt cgtgaggaat caacatgatt ttggttctgg aaggagcgtt gttcctcgcg
     4081 atcgtcgtgg cgatggtggc catgcttttt cgactgccct gtcccaaaga cacggctctc
     4141 attttgacgc ttcggtcgaa tggactgccc ttgcggtcgg gagagcccat gtttcgcgtg
     4201 ctcgtgggca aagccggctt tcgttccccg gccgttgcgg cggtggattc gctcgacctg
     4261 cgtgcctttc ccgtcgaagc gttgctcatg atcgacgagc atgagtggct ggtgacaatg
     4321 cgcgcgcatg tgggagggca agagtcgatg gtgcgacgag ccgcggttcg gctgttgggg
     4381 atgtcacaac gcgataggga agcgatcggc gagggggtat tggcgctcgc ggttcgtgat
     4441 gcgctggtcg gtcgtggcgg gggcgaggtg gtggaagagc aacgggaagc gatcgaaaaa
     4501 gacgttcgat cgcgtgtggc cgcggtgttg gagccgatgg gcttggaggt tttggagttg
     4561 tcgttgacga tacggcgacg ggaaaagagc gacacggggc cacggtaggt ggtgtggcat
     4621 gagcgaaagg ggggtggtgg gcgaggggta ggaatggcgg gaggaggagc gaaggggggc
     4681 tacttgcctt cctccgactg ataatatttc ataaggattt cggcaacttc cggacgagcg
     4741 aactcaggtg ggagggcttc gccggcggcg agcatggtgc ggaccttggt gccggaaagc
     4801 aggacgaagt catccttggt gtgtccggga ggggcatctt tcatcatgat gactttgcca
     4861 agctttttgg agaaagcggt gtggtccgcg cggtagatct cgatttcgag cgagcctttg
     4921 gggatatcgt cgaaaatcgt ctgcgcgtcg aagggaccgt agtaggaacc gacgcccgcg
     4981 tggtcacggc cgacgatgag gtgcgtgcag ccgcagttct ggcggaatac ggcgtggagg
     5041 acggcttccc gggggccggc gtagagcatg tcgtagccgt agccggtgat gagaacggtg
     5101 ttgggcggga agtaaagttc gaccattttg cggatggcag cgtcgcggac atcggcgggg
     5161 atgtcgcctt tcttgagttt gccaagcagc atatggatga ggatgccgtc ggccttcacg
     5221 tcggcgtggg ccatgcggca aagctcctca tgggcgcgat gcatcgggtt gcgggtttgg
     5281 aaagccacga ctttgttcca gccgagcttt tgcatctcgt cgcggatttg gtaggcagtg
     5341 cggaaggttt cggggaactc gacgtcgaaa tacgagaagt tcaggacctt gatggggccc
     5401 gacacgagca ctttgcccac ggtcgtgaac gcggcgactc cgggatgctc cgggtcgagg
     5461 gtgcggtaca ccttttcggt gatcatcttg agttcgtcat cggtaagttc ttcgatggct
     5521 ttgacatcca tcacggcgag cacggggttg ccttcgacgt tggggtcgcg caaggcgatg
     5581 cgtttggcgc ctttgatggc cgaagcgtct tggatcatat tgacgacggg gacggggaag
     5641 aacacaccgc tggcggtttt cataccctcg gcgacactga gggtatcggc tttgttcatg
     5701 aagccggtca gggggttgaa gtagccgctt ccgagcatga cggcgttggc ggcggcggcg
     5761 gagcaaacga cgatggaggg caaggattcg gcctctttgg ccaacgcgtc gcgctccttt
     5821 gcatccatga cgaacaacgg attcagcttg tcggacccat gcggtttaat catcctgctc
     5881 tccttcagcg ttttcacgtg acgaacacgc cacgaatccc tctttcccca tcttgcttgt
     5941 gtctggtgga taccggactg caagcgaggc atgtggagag cgctccacta ccacttttat
     6001 tcctaggtcg tcaaggcaag gctgaaggca atcacctatg attttcagca attttttgta
     6061 ctagactgat gaatcatagg gcgatgagca aatatagctt tagctaacaa taattggctc
     6121 agataaaaat aaaaatagta tggattatta tatacttata ggtccatcgg acggaggcac
     6181 tgccagcatg gaggggttca atccgtttct tcccagccga cttcatcgag tttgcggtaa
     6241 atcgtgcggg tcgagattcc gagcaattgc gcggccaggg acttgtctcc ccgcgtatga
     6301 cgaagggttt ctcgaatgag tttccgctct acttcgtcga gggaggttcc gacggggata
     6361 gacagcacat tgggggtgag gttggagccc tgggcgatga cgtcgggcag gtcttcttcg
     6421 gtgaggaccg tggttttgca taggacgacg gcgcgttcga tggcgttttc cagttcgcgc
     6481 acgttgccgg gccagtcgta atccatgagg gcttggagca gaacgcggga agtctcgagg
     6541 cgggggcgac cgtttttcgc gcaatacacg ccgagaaaat ggtcgacgag aagaggaatg
     6601 tcctctcttc tggtgcgcag ggggggggcg gtgatggcga tgacgttgag tcgatagaac
     6661 aggtcttcgc ggaaacgtcc cgcggcgact tccgcgcgaa gatcccggtt ggtggcggcc
     6721 acgattcgaa catcggcgcg ttgggtatcg cctccgatgg gttcgaattc tccctcttgc
     6781 aaaacgcgaa ggagtttgac ttgcaccgac gccgacagtt cggcgatttc gtcgagaaat
     6841 aaggtgcctc ccgcggcttt ggcgaaacgt ccttctcgac gggcgatggc tccggtgaac
     6901 gctccttttt cgtgtccaaa caattctgct tccagaatgg actcggggat ggcggcgcaa
     6961 ttgacggcca cgaacggacc ggtgggtctt gcgctttgat catggatgaa ccgtgcaatc
     7021 agttcttttc cggtcccgct ttctccaagc accagcaccg tggccatcga gggagctgct
     7081 tgcgtggcga tatccagcac ccgtcgcatc gcgtggctat tgcccacgat ctcacgatgt
     7141 cgcaatcgct tcaattctcg cttgagagtc cggttttccg tgagcaaaag ccagcgttca
     7201 gcagcttttt tcaggctttt caccagcgtg acccgtttga gaggcttttc gatgaaatcg
     7261 taagcccctt cgcgcatggc ctgtaccgcg gcttccaccg tgccatacgc ggtcatcacg
     7321 accacctcgg tatccggcga tatctctttg acggctcgaa gcaattcgat gccgctcgta
     7381 cccggcatca tcaaatccgt gagcaccacg tgaattcggt gccgtcgagc caattccagc
     7441 gccgtacgac cgttgggagc cgtcaacgcc cgcatgcctt cccgctgaag gatcttttct
     7501 atcgattcga ggtgggaagc ctcatcatcg acaaccagga tggtggtagc gcctgggggg
     7561 acttcggtct ccgacaaagg cgacggcgga aaattgggcg agtcatgcac catgtcatct
     7621 tgtcaacacc gtgacaatct gtccaattcc tttcgaaccg cgaaaaagta ccgggggcct
     7681 gaagactggc ttggaaggca ggcttgacag aatcgatggg tcgcgtcatt gaacgctgcc
     7741 ggtcgcctta tggttgcgac gtgaacatgc catggaccag ttttcagctc atctcgatcg
     7801 cggctgggac ctcgtacaaa agggggatac gcgaggggcg gaggcttccg ctcgacaggc
     7861 tatcgaactc gaccccaatt caccggaagc ctacaacctt cttggttttg tggctgccct
     7921 cgaaggtgat gcggatgagg ccatcgagta ttacagccag gccattgcgt tggacgagac
     7981 gtatctcgaa gccatgctca atgcggcgga ggtttatatt caccctctcg cccaatacga
     8041 cgatgccatt tccatctgtg agcaagcgtt gaacctcacc gaatccgatg aggaggagat
     8101 tgacacgtta ttgctgatgt tcgatgcaat gttgggcaag ggcgacgtgg tatcggcgag
     8161 gacggtggtc cttcgtattc cgacgggtcc ttatgagaat ccgaaccata cgtttttggt
     8221 gggtcgggcg ctgttcgaga ttggtgagat tacgagggcg gcgccgttga tcgaagaggc
     8281 ggccaacaag gatccgatgc aaagcgaggc ttggtactat cttggattga tacgcgatga
     8341 gcgtggggac ccacgcgggg ctacggaggc ttttcttcgt gctcgggagg tcgacttgcg
     8401 cgaacctccg ctgccttggt cgtcgccgcg agaccatttc atgcttgccg tcaccaaggc
     8461 cctgcaagcg ttggcaccga tgctccgggc gtttgtcgac caggctgagg tttttctatc
     8521 cgaggtgccg ggcatcgagt tggttgcgga tggtgtcgac ccgcgggcgt tgctactgct
     8581 cgacgcgttg gcgactccgg agctgccagg accgccctgc ggacgcattt tcgtctacca
     8641 gcgcaatgtc gagcgtgtag ccggctccct ggaccgtttg gaggcaatcc ttgtcgaagc
     8701 cttcgaacgg gaaatccaag cagttttctt ggagtccacg agcgaaccgc cacccgcggc
     8761 cgtgttgaat tgatggttct gggtgagatc caccgtaagg tcatgacggg tcgcggcgtt
     8821 gtcttttcgt gacttcgttg gccttgccgt ggatctcgat cgatgggttc cccaccatgc
     8881 gttgggggtc agaaaacgag cgctctttgc cgcaaacgag taaggtcacg gctgtgaccc
     8941 catcggatgc ggcagacctc ttggatgagc cgcggctcgt gcagcgcgcc aaagcgggag
     9001 accgtcgcgc cttgggtacg ctcttgtcgc atcatggtcc gcgcttgtat cgcgccgtgt
     9061 tgctccctcg gcttggcaac gaggctcgtg cgcgcgatgc cctgagcatc acgtatgaga
     9121 aggccattca gcacatgcct cagtacgaat ggcaaccgtg tggcatctac ccatggcttc
     9181 gggtcattgc gcttcgaacc gccctcgaca tgctccgggc gcgtcgacgg gaggtattgt
     9241 tcgatccgca agacgtgagt cgcgaactcg agtccgcaca agcctccgta tcgtcttcga
     9301 tgccgggatt ggatgctttt catgagcgag acgaactcga tgccgtccgc gcgatgctgc
     9361 acgatgcctt ggcacgaatt catccgcggt acgccacggc aattcggctt cggattttgg
     9421 aagacctccc gcgcgagcag gtcgccgagc agatgggtgt tactccagcg accttcgacg
     9481 tgttgctgca tcgctcgatc gcgtcgttga aaaaagtgat ggacgttcga ggcacggaag
     9541 ggtcggagcc atgacggtgt acgacgacaa gggttgggaa gagctgacgc caccgacgcc
     9601 acaggaagtg gagcaagcac aagccttgcg agaggcgttg gaggcaggcg aagaccatcc
     9661 cgatgcggac ctattgcgat cgttgcgttt tttggaaaac ccgccacccc tcgaaccgcg
     9721 ggtgaatgaa tggctcgttc acagagcttt gcacaagatg gcaccttccc atcgggggcg
     9781 ttgggtagcg agtggtgttg ctgccgcact agccttggca gcgggagttg tcgtcgtgtt
     9841 gtccacgggg cccgatccca cgacggcgcc agtggttcga gcgagtctgg tgtcttgtcg
     9901 atcgacgcaa tctttgtttc atgagccttt cgaccgacat gggggcacat cgagccgcat
     9961 ggatcgaatt gcccgggata gagcgcgaga ttaccgtacc aacctgtttt tacgcggggg
    10021 ggtgcaacca tgacaaccaa gttgattcgc ctcgtgtttt tgctggggct cctttggggg
    10081 atgggatgtt cgcagcgttc ggacacgccc tgcgtatgtg aggcaacgcc ggtggtggac
    10141 acggcgttga tggcctttct ttcgaaggcc aaggcggcgc atcatcaggc ggatcttctc
    10201 gagcaatcgg ggcaggtgga cgcggcgatc gaggtgctcg acaaggtgac ttcgttcacg
    10261 cagatgagcg accgagcgga agtgcgggaa gtgctggcgg acactttggc gcgcttgtcg
    10321 gacttgcgag gacagcgtgg acagtnnnnn nnnnnagtgg gaagagtccg aagcggatat
    10381 tcgcaaaggg ctcgaattgg ctccttcggg cagctacttc gaagggcatt tatacgaaat
    10441 gcggggtgtc aatgaggaaa gacgcgccaa ggcgcaagcc gagcaaggcg acgctgaggg
    10501 tgcgcaaacg gctcgtcgac gggcgattga agctttcgaa cgtgccattc aaatccagga
    10561 ttcggtcatt cgcagggaat tgagcgatga gggggccgac cgatgaaaac gctatccatc
    10621 cttcgggcgg gttgtatttc ccttcttttg atgggttgtg cgcgtgcccc ggtctatcct
    10681 gcatcgccct cggcccctga atcgtcgggg caagcggtcc aggaagtatc ccgcccagag
    10741 tcgaccgcgg agttcgagcc gccggcagag cagtcgcgcg cgccttcgga accatcttcg
    10801 ctacccgtgg cgccgtcgac gaagcgcaac tatgcgttgc ccccggattc ttacgacggg
    10861 ggtcacgagg gggtccgcag tttgtgggag gagctatcgg cggccgaaca ggttgcctta
    10921 tcggtgcggt ctggatgcga acgagcgtgc cgagcgttgc tgtcgatgca tcgtgccacc
    10981 gaaaggctgt gtgaactggc gtccgacgag ccagaaaaaa aagcgtgtca gcaagcgcgg
    11041 cagcggcttc gcgaggccaa ggagacggtt cgaaggagtt gtcagcggtg cgaacagggc
    11101 ccgtcgttga cgtcggacga ctaggcttgt ctgcctgggc tggcgttgta tcggctgttt
    11161 tctttgggga tatggtgtaa gggcaagggg gtgggtgacc ttcggctcgg acagtcttgg
    11221 acgtgggcac agcgctggaa aaacgaattt ttattccatg cggccacgct cggattgtgg
    11281 tggggtcgtc gtctttcgga gcctgcgcga caggcgttgg gaagacgatt ggggagggtt
    11341 ggggcgcggc tgtggacggc ggggatgcga cgggcggggc atcgcttgcg tcaggcgttt
    11401 ggggaggcca tgccggtgac gcctatcgag gtttttgaga cattgggcgc ggattatgcc
    11461 gataccttgt cgctgatgca tcggtctttc agggctgcgg agcggatgcc gttggcgtct
    11521 ggggcgaagc gagcattgga cgaggcggtg gagcgtggaa agggggtcat atttttgacg
    11581 gcgcattttg ggccgatgga tatgatggcg gcatcggtgg cggagcgggg gtatcgtgtg
    11641 gccaccttgg cgcgtgagag ctatgatcct cgtttcacgg cgttttttga ggcattgcga
    11701 gagcctcggg gtgtgcgggc gatttaccgg ggacgagcgg gagcggagat ggcgatcctt
    11761 cgtgcgctgc gagatggcta tcttattgga tttccaattg attttgcagg tcggggcatg
    11821 cgcacccgag aggtttcttt cttggggcag acggttccca tggcggtggg acccgcggcg
    11881 ttggcgttac gttttggcat tccggtggtg gtgggggcgc cgatatcggt ggcagggggg
    11941 cttcaggttt ttgtggagcc ttttatggag ttgcaccatg gggatgatgg tgcgacggtg
    12001 ggagctatgg cgagggcttt ggagcagcga attcgggatc ttcctgctca ttggccgtgg
    12061 atgcacgata cgacgggcgt cccacgcagc agagcattgc ctgatgtcaa gcgttgtcag
    12121 taaactgaac catctttttc gtgaattcgt tgatgactgt tgagctgatc gtgacaaatc
    12181 aacccttgaa tccacgtgtt ccacgcctca ttcccgagtg caatcttatg acgttgcctc
    12241 tgacgcctgc ggaggcgttt ttgctatcgc gcatcgacgg ccatcaaacg gcacgggacc
    12301 ttgcgatgat gacggggatc gatgaggcca tgctgatgga atcgctcgat cgtcttgcgc
    12361 aaatgggggc tgttacgtgg gacggggcgg agccggcgga gcgcaaggag cgtgaaaaaa
    12421 aatcggacgc ggaacgggtg gtgcggtcca ttccctctcc ggtggtggaa gagcgtgtgg
    12481 tcgatccgcg cccagaacca ttgccccttt acgacccgtc cgaactcgat gagccctgtg
    12541 atttgcccga agataagcgt cgtaccattc tcgacgcctt ctatcgattg gaggacatgg
    12601 attattacga gttgttgaat gtaccgtcca cggccacgaa aaaggagatc aaggattcct
    12661 attatcgttt ggtggcggaa tatcatcctg accagtattt tcgaaagaat ctcgggtcat
    12721 tcaagcaaaa gatggagact gtattttcgc gtctcaccca agcgcacgac acgttgacgc
    12781 gcaagcagag ccgagcggaa tatgattcct atttgtcgac ggtgcagagc acgcggcgtc
    12841 tcgaggagtc gttacggtct tcgagggcaa cggtggatga ttcctccacg ggtgaggcca
    12901 ggacgcggga tgagcgggtg gagccgtcgt cggtgctcgg ggctgggcgt cgtgagatgg
    12961 aaacggcgcc ggttaggcga ggggagacgg aggaggttaa gcgagaggaa acggttttgc
    13021 aggagccttt ggtatcgggg cagcccatgc ggagcagtgc gcgaccggca ccgacgcgtg
    13081 agagcatgcg tgcgcgcagg gaggcgtttg cggcgcgaat gtctgggggg cgagtgagtc
    13141 ggataccgcc gccgccgacg ccatcttccg agcctcccgc gcgtccttcc gttccgcagg
    13201 gtgtggcggc gggggaggcg ctgcttcgtc acgtgacgga gcgcaaggag gcggtgcgtc
    13261 aggcgcaaat cgacaagtat ttgcaagcgg ccaaaacggc attggagcag ggggattttg
    13321 ctgcggcggc caatagttat cggcttgccc tccacatgga gccggacagt gcggagatcc
    13381 aggaggcttt tgaaacgacg caagccagag ccacggtgga gttagcgggc agctatctac
    13441 agcaagcgcg gtatgaggag cgagcggagc gttggttgga ggcggcgcgt tcgtattcac
    13501 gggcggccga tggcatgctg agtgacgcgc aggtgcagtc gaaagcggcg gaagccatgt
    13561 ggaaagccaa tttggacctg cgaaaagcgg cggaatatgc gaaacgggcc ttggctctcg
    13621 attcgaataa tcctgaatat cacgtaattc ttgggaaaat ataccttgcg gcaggattgg
    13681 tgctcaacgc caggagagag ttggagttgg ctaacgaact ttcgccaaac gatgctacca
    13741 tcgccaaact gctcgaggcc gttcgcaagg gcggaaaagc ataatagaaa gggtccctga
    13801 atggatcgtg tcatcggtat cgacttagga acgaccaact cttgcgtggc gatcgtggac
    13861 gatgggacgc cgacggtcat tccgaaccgt ggaggataca agacgacacc ttcgatggta
    13921 gcggtgacgg aagcgggcaa gcggctggtg ggtcatattg ccaagcggca agcgatcacc
    13981 aatgcggaga acacggtttc ggctgccaag cggctcattg ggcgtaagtg gaactcttcg
    14041 caggtcaaaa actccgtgct tacttcgagt tatcagatcg tggaggggcc ccatgcggac
    14101 gtgcggatca agcttcggga caatagttat tcggtccctg agatcagcgc catggtgctg
    14161 caggagatgc gtgtcattgc ggaagatttt ctcagtgaat ccgtgagcaa agcggttatc
    14221 acggtccctg cttacttcaa tgacaaccag cgtcaggccg ttcgggatgc ggggcaaatt
    14281 gccgggctcg atgtcattcg tatcatcaac gagcccacgg ctgcggcctt ggcctacggg
    14341 ttcggcaaaa attacgaaaa gctcatcgcg gtctacgatt tgggaggcgg cacgtttgat
    14401 atttccattt tggaaattgg tgcccacggc gtgttcaagg tgattgcgac ggcgggagat
    14461 acctttcttg ggggggagga tttcgatgcg ctgatcatcg attggctcgt ccaatcattc
    14521 aaggagcagc atgacatcga tcttcgacaa gatcgtatgg ccttgcagag gctaaaagac
    14581 gccgcggaaa aagccaaatg cgaactttcc tcggtcaaag aaaccgaaat caacctgcct
    14641 ttcatcatct ccagtgggcg aaacgaagcc ttgcatcttc aacaaaccat atctcggcaa
    14701 acgctcgaaa cgctttgcga ggatctggtc cagcgaacgg tggagatatg tcgtcaaacc
    14761 atgttggatg ccaggctcga caaggatgaa atcgaggatg tcattctcgt cgggggaatg
    14821 acccgcatgc ctcgtgtgca agaagctgtc gccggatttt tccaaaggga gccttgcaag
    14881 ggagtgcatc ccgatgaagt cgtcgccttg ggggcggcga ttcagggagc ggctttggtg
    14941 gatgatgccc atgaaatgat ccttctcgat gtcacgcctc attcgctggg catcatgacg
    15001 tttggtagtg tctttgaaga actcatcgcc caaaacacca cggtgcccac cgcgcaaacg
    15061 aaaatcttca ccacgagccg ggacaaccaa accgcggtca agattctcgt catgcagaaa
    15121 gaaggtcagc aggcagacga cttcgagctt ctaggagaat tcattctcac caaccttcgt
    15181 cgcgcaccca agggccaagt ggaaatcgag gtcacgttcg aaatcaacgc cgatgggatt
    15241 gtcaacgtct cggccaagga tatggaaaca ggacagggcc agtcgattca ggtcacagcc
    15301 agcagtggac tgaccaagga cgaaatctcc aacatgatcg agtccgccaa ggaatacatg
    15361 gtcgaacgac gatccagcga agagttcgag gcggccaggc aagaagccga gactctcatc
    15421 gccgaaatcg aacgtctctt tccacaagtc gaagtggtcg tggccgcaag tgacttcgga
    15481 agagaggcgg tgaacaaagc gcgtaggatt ctcaaagacg caaaaagcgc catcgagagc
    15541 cgtgacttga atcaagtcaa atcacaaacg gaagcactgg ctcgcacgca tcggatgttc
    15601 aagggagtcg tcaccaagac gcaataacga gtatggcgaa aaagccagat acaacgtcga
    15661 taccaccgga taccggcgag atcgatactg attggagcga ggcttcgttg cagcagagcg
    15721 cggagggatc gggaattcct accgcggtgt tgcctgaacc ttacgagcgt tacgcaaagc
    15781 aagtcaccag ccaaccgatg ggggaggaaa cgcgaaaagc cttgtccgcg ccccggcttc
    15841 cggcgatcga cgagtccgtg ctcgacgaat tcctcgaaca gcatagtcgt gactcaggcg
    15901 aaatgagagc gatggggctg gtggggcgtc agctttctgg cccttatgcg attcctctcg
    15961 aattgaatga tgaaggagag ccggttcggg caagcgagcg agcgatggaa tcaccaagcg
    16021 agttgctcag cgattcgggg gccgtacgca ccacgcccgt gccttcttcc aaggattgga
    16081 ccgccgagtc tccaactccg cacaagccgg tcccggaggt gctcgaggct tttcgacgcg
    16141 cttcggcgct ggagcctgct ggttccgaca acgatctcgt cggtcgtgag gagtacttcg
    16201 agttggatga accgggagag attggcatgg aggattccct cgaaatcaat ctcgacgagg
    16261 tagttccttt gcctttcgat ctatcgcagg cgttgcaagt cgaccgaaag ccaacccctc
    16321 gacccgaacc cattgacccc atcgaatttc ctctcgaaaa cgatggtggg gccctcgatc
    16381 tggtgtctgc ccgagcgact ccgttcgtgc cgcagcctac cgatcccatc attgacttgc
    16441 gtgaccgttt cgccgtgggg gacttcacgg gagcgcatgc cgtggcggaa gccatccttg
    16501 ccgagcaacc caacaatacg gaagccctcc gttttcgaga aagctgtcgg gatgttctca
    16561 cccaaatgta tacggcacgc cttggacccc tttctgggat tccaaaactt gccatcccgg
    16621 tcgtggagct acaatggctc agcttggacc atcgaacggg ctttcttctg tcttgtgtcg
    16681 atggccaatc gaccatcgag gagatcctcg atgtctctgg catgccgact ctcgatgctc
    16741 tcagaatcct ttttacgctg cttcagcaac aagtcatcga aatcgtgaat cgctgacgtt
    16801 tccccttggt tcccatgcct gctatctgca ccgaatcgat tcgacgtatc gctcgtagcg
    16861 cgagccttca tctcgaaaac gacgaggtgg aaaacatggc gcgcgacttg tcgaaggacc
    16921 tcgaagccct gcaagggttg cgcgaaatcg acttcgaagg cccgtccttg gacgatgaaa
    16981 aaaacgttgt attacgaagg gatacgccgg agcaaagcct gtcgatggag caagtgaaaa
    17041 gccaaagtga acgggctact ccggaggggt ttcgcgtgcc acctttcctt cctcacgctt
    17101 gacctattcg cccatcgact cgtaggttgg ctccgtgaag gctgtgcaag acattctgaa
    17161 ttccccagtt caccgtcttg tggaacaggt tcgagaaggc agtctgaccg ctgtcgatat
    17221 tgccaaagcc tttgtggaca acgcgcgcac gggggaccct cgcattcgcg ctttcgtgca
    17281 tttcgacgac gcatccatcg agcggcaagc gaccatcatc gatgaaaaac gaaaacgagg
    17341 tgagcccctt ggcgcgttgg caggaatacc ggtggccatc aaggattcga tttgcacgat
    17401 cgatgctccg accacgtgcg catcgcgttc cttgatgcat gggtctttgg catggcgaag
    17461 tcctcatgaa tccacggccg tggctcgctt gcgtaaggcc gatgcgatta ttttcggaaa
    17521 aacgaatatg gatgagttgt cgatgggttg ttcgacgact cgaagcgctt tttttcctac
    17581 gcgcaatccc atctgcgaag aacttgtgcc gggggggtct tcggggggca gtgcggcagc
    17641 ggtggcggct cgtatggttc cgcttgcctt gggaagcgat gcgggagggg gcattcgaca
    17701 gcccgcatcc ttcacccata cggtaggaat caaaccaacc tacgggcgtg tgtctcgatt
    17761 cggactcgta ggggtggctt ccagtatgga gcagattggt cctttggcga gaagcgtgca
    17821 agacgccgtc cgactcttgg atgtcgtggc tggcagggat gagcgcgatg ccacatcgat
    17881 gcaagcgtcg acatcgccgt tggagccgta ttgccatcag agtatggacg gccttcgtat
    17941 cggcgttgcc cacgaatacg agcaagatct tcctgatgat atcctagtgg ccgtgaagca
    18001 atcgttggaa gccttggaat cggcgggatg tcgtctcctg ccgatacgct tgcctcacac
    18061 gcatcttgcc ttgtcttgct atcatgcgtg cgttgctgcg gaggcttcga gcaatctggc
    18121 gagattcgat ggcattcggt ttggtgccac ggacccgggg ggagtggatg tggagcagtg
    18181 gcgcggttgt gttcgtggac ggggattggg ttgggaagtg aaacgccgag tcatgctggg
    18241 aacgtatttg ttgtccgcgc agcatcgcga agggcttttc gagcaggctc gcaaggcgcg
    18301 ttgtctcgtg gcgaaggact tcgacgaggc gttttctcat gtcgacgcca ttgccacggc
    18361 cacatcgccg atgtttccgt ggccgatcga aatcggagat gaatcgatgc atcgggcgga
    18421 tgctcatacg gtggccgcgg gtcttgcggg gttgcctgcc ttgtcggtgc cttgtggcgt
    18481 tgtttcgcaa gtgctgccaa tcggcttgca actcatcggg cccgcgtggc gggaagatat
    18541 ccttattcgg atagcgagca gatgggagca acttcgggaa ccggcctcct tcgagagaga
    18601 aggctaatga tttcgtggcg ttacacgttt ttaggagtga tggctgtcac ggtgttgggg
    18661 gccatggggt gctcgtcgtg tcgaaaacag cagcggcatg atttcgattc ctctgcgcca
    18721 atggcatcgt tcgaagtggc gccaggacga cttttttctc catggttttg ggaacctacg
    18781 gatgctcggg acgtggcgct tcccgcgggt tgcgagttcg tgggtccacc ccttcgacat
    18841 cgtcgtagca ccacgaacct gttctttttc gcttctccgg gagaacttca tactcttgcg
    18901 atggctcaag gggatcccaa tccggatagt gttacggaat ccagaggcct tgtgactctt
    18961 tccgataagg gggcaggtgc cgttcgcgac gtcccttggt tgcatctcac cgacccgcct
    19021 ctgatgacct catcgtcgca aggttggctg gcgctgctgg ttgagagcga tggtttggga
    19081 ttggccacac gattgtggtt atggcgggaa gcgggggcca tggaattggt tgcagaaggg
    19141 gatcgacttc agcccatcga tgcgcaatgc aatcagaacg gatgtgcctt gttgacgaca
    19201 cgcgtggggc aggtggccac gcgaggagcg acggcatggt tgggtcaacc catggcgccc
    19261 atggccacgt ggctacggtt ggaaatcccg ccggatgccg ttgccgacgc atcggttccg
    19321 cttggtctcg tgagctggga cgcatcgtcg cattccgcgt tgatcgcctt cgaggattcg
    19381 gatcgcattt cctttttgcg tgtgactgac acgaaggttg aacgacaggg atcgatcgaa
    19441 cgggactatg ggattgtcga ctatcttccc atgcgggatg gccccatggt ggtcacgacg
    19501 tggggtgagc ccgacgatat tggatgtgtg cccggtggtg ctttcgtgca attcactact
    19561 ttgggaaagc aagctcatcg acttcgcgta gcgatgttgc catcgtcggg gtatgcgcga
    19621 gcgttggaac atggcgcgtt gataacctgg agtgcgccga tgcaatgcaa ccttccgaac
    19681 cgaaaggtgg tgtatgccgt cttgttgcat gcggatggcg cgccggcctc cagcatcatg
    19741 gcggtgggca acgccgatgg gcatgcggtc agtacgcgcg gaaacgaggt tcacttgtgg
    19801 ttgagtgatc tcgtaggagt gacctggctg cgtgccaagt gcaggccacc aatgtaacac
    19861 cctgcttttc aggagtcggt ttcgacctct gccatcttac aagttccttc gaaatgcacg
    19921 cctttgtcga tgaatacgga aggagaatgc agcgaagccg tgacatgggc gggaacatat
    19981 aattcgatcg agccacgagc gcgaatggcg ccacggacca cgccgccccg aatgatgacg
    20041 ttatcgacat ccacttcggc atcgacctcc gccccatcgc caatgatgag cgtatcattc
    20101 gaacgtatct gtccacgaaa actgccgtcg atgcgcagac gaccgtcgaa cagcagtttt
    20161 ccttcgaaat gcgttcctcg tccgagcaag gcggtgattt cggaagcggg aagggtatcg
    20221 gtattcacgg aagcttcctt gccgtcgagc ctttcgatga aacgtcgatg atacgatgaa
    20281 acgaagtctg gggcccgggg ctcgatcccg ttgtagcgag attatgccgt ggttcgcaat
    20341 gatcatggtg aggggtctgt cgagagggaa ggcttcgttt ggggtgggcg tatgactttc
    20401 gtacgcattt t
//
LOCUS       DAOSOR010000029        20305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_2633_length_20305_cov_4.095160, whole genome shotgun sequence.
ACCESSION   DAOSOR010000029 DAOSOR010000000
VERSION     DAOSOR010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 20305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20305
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2633_length_20305_cov_4.095160"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..1306
                     /locus_tag="PK710_02845"
     CDS             92..1306
                     /locus_tag="PK710_02845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOT08676.1"
                     /translation="MKKGWVPRRKKRRYTRSEMPERTIFHVDMDAFYAAIEQRDHPEL
                     LGKPVIVGGVASRRGVVSTASYEARVYGVHSAMPMAQALRKCPHGIVMPVRMSHYIEV
                     SRQIMAVLHSFSPLVEPLSLDEAFLDMTGTQALHGSAETTAKSIKQRVLETTSLTCSV
                     GVADNKFLAKLASDLHKPNGITLVPPGEGASFIAPLPIRRLWGVGPRTAQRLEKIGCK
                     TIGDVASIQSGWLVRVLGEASAEHLQALARGHDDRPVIPDREAKSIGSEETLDRDITG
                     QGAVLLLVRRHCERVAFRLRQAGLQARGIRVKLRYQKGFRLTTRETTWAQPCDDSRTM
                     YRAALGLVSKLDVSEPIRLVGVGAVHLEPSGVTKQGDLFEHGSSKKDSRLEHTLDAIR
                     MRFGDVIRRAQD"
     gene            1454..3709
                     /locus_tag="PK710_02850"
     CDS             1454..3709
                     /locus_tag="PK710_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08677.1"
                     /translation="MKQKLSWVAVAAVCGGAVLGACSGGNSDGSASNANTHPDGGASE
                     ASADGALPVDGAEGSVFDVTSSDAHDITIYPKDPVITVSTGQPIPSLTFEARVNDKSV
                     TALWLVDRGEIGLIDVHSGVFEPGGAIAGVAKVVATVGTEDVSTTVTVKISVVQNGGT
                     GGSGSGGVGGEGPGVPVNAAIQGILEGATTADPNFRWLYPYDGTVWPLGLLSPLLQWT
                     QGAAPAEAVYIHLSSEHYDYKGFFGLPPALPPGAPLVRHPIPQEVWKAATTSNAGGTL
                     TASLVVAAGGTAYGPISQTWKLASGPLKGTVYYQSYGTALAKNYGGGIGGDGQFGGAT
                     LAIRGDSSEPTLVAGSNGGLKECRVCHSVSADGSRMIVQRGDDYAASTSFALTAGYSE
                     TIYPVADREKFGWVGLTPDGALGLGNAAPLYAGAPTSSQLYNMNTGEVIASTGFASFV
                     TKAGFPAFSPSGGHVAFSLFEGPGNAATGPGDGSQLIAMAFDRATATFSDPKLLFQAT
                     AGTAPGWPSFLPTLDAVVFSVQSRLNDAGEFMMTRYGGRGQLWWADLATGTAHPLDRA
                     NGSDGTNSYLPTGPNGHDDDTSLHYEPTVNPVVSGGYAWVVFTSRRMYGNVATINPWA
                     SDPREHDLVANVTPKKLWVAAIDLNAAPGTDPSHPAFYLPAQELMAGNTRGFWVVDPC
                     KSDGEPCESGDECCGGFCQMDGDAGGLICMNHSNECSNEYEKCAQDSDCCDFLTGTKC
                     INERCANVVPK"
     gene            complement(3744..4793)
                     /locus_tag="PK710_02855"
     CDS             complement(3744..4793)
                     /locus_tag="PK710_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08678.1"
                     /translation="MKLLQLRVVDLGPFADVVVSFRGPEEQARNVTIVVGGSGVGKTT
                     LLAAIASTRPAHAVAAMARSQAATPGYVIADWDLSDDDPERPHPLQVCSPHTPVKDTE
                     EDRLRRREQTLFDRRAVEQGGFMVMSMSSTRWFSRASVSLSSPERTVLRYDVRAAQSF
                     DDASHADIARDVKLALVYAAIASRIPEPDSGRLSLSLAVVDEAMRDLLARLLALVSVR
                     YLGVHPLTLEPVFATDRGEIMSFDRLPTSIRHLVAFAVLPVRALAAAYPGRDPRMTEG
                     VVLIDEVALHQDPYVQRGIVAALRGALPRVQWILTTSSPEVAWACEPYEVIALRRMPS
                     SRRVELFEGPLATLH"
     gene            4971..5831
                     /locus_tag="PK710_02860"
     CDS             4971..5831
                     /locus_tag="PK710_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOT08679.1"
                     /translation="MISYPPPPRERNVEVLREIARKIRVSIVRMIHKARSGHTGSSLS
                     CADILTCLYCAEMRHYPLRPDWKGRDRFVLSKGHAAPALYAVLHHCGYIAQSDLDRLR
                     SLSSILQGHPDSRRCPGVEASTGSLGQGLSIAHGMALALRGEQENPRVYVLLGDGELQ
                     EGQVWEAAMSAAHYRTSNLCAFIDANGLQIDGPLEGIMGVEPMVDKFAAFGWNTMEID
                     GHDVGSILSALDSARKQTHAPSVIIARTTKGKGVSRFENKVQWHGKAPGDDDLALALM
                     ELGELDKEAS"
     gene            5828..6793
                     /locus_tag="PK710_02865"
     CDS             5828..6793
                     /locus_tag="PK710_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HOT08680.1"
                     /translation="MNGRGVSPRILATREEYGRTLVRLGERSRDIVVLDADLSKSTCT
                     HEFAKRFPDRFFNMGISEQDLIGTAAGMALAGKIPFVSSFAIFLTGRCWEQIRQSVAL
                     PGVNVNLVGTHGGVTVGKDGASHQALEDIAVMRAIPSMTVIVPADAVETRKAVEAAAW
                     YDGPVYIRLTREKLPVVYPDEKPFRIGAGAIVREGSDVTLCAIGLMLHVALEAAERLA
                     MESISARVVDLATVKPIDEFLVTKCAQETGCIVTAEEHSTIGGLGDAVGEVLLRRRPV
                     PMERVGVRDAFGRSGSAHELLEYFGLTPQAVYEAAKRAIERKAAH"
     gene            6820..7752
                     /locus_tag="PK710_02870"
     CDS             6820..7752
                     /locus_tag="PK710_02870"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOT08681.1"
                     /translation="MVSLKLFTGSSNPELAHKIAAELEIDVGDADVTRFSDGEVDVRI
                     NESVRACDVFIIQSTSYPVNDHLMELMLLADVCRRASAATITAVLPYFGYGRQDRQTQ
                     KRVPISAKVVANMLTIGGVDRIITVDLHAEQIQGFFDVPVDVLRSARLLVDNVHQRGL
                     RDLVVVAPDAGGVEIAGRFAMGLGATMALIDRHRDAPAKVKAMRLIGDVEGKDCLLVD
                     DMIDTGSTLTEAADLLITSGAKSVRAAAVHPVLSGSGVERLHRSRIDELITTDTIPLP
                     VEKQIEKVTVVSMASLLAKAIRHVHVGKSLSNVE"
     gene            complement(7818..9173)
                     /locus_tag="PK710_02875"
     CDS             complement(7818..9173)
                     /locus_tag="PK710_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08682.1"
                     /translation="MKYSRICWVLGVAMLWAIGCSDSEEEGNTKPGGPAGAGGSVGGS
                     GGSTGGTGGTAGHTGGASGSTGGSSTEGGSGGNTSGAGGSVSCEQGCDDGLACTSDAC
                     VNDVCEHTIEQGWCVIQHACVQQGATNPSNPCVACDPAKTTSQYTALPDGTPCGGSQT
                     CLKGQCGGAPPAGRVIGHENTRLDLIPSSAISAAKSKLHIAYQHTSHGSQLITGMNAL
                     EAFPSFGNTYAWDDAGQAPNALDLDDNGIPGCADLSQGDSVDSHGDTPWVIATRDLLN
                     KSSNSHINVVMWSWCSINGHDAQRYVDNMEKLVKEYPAVDFIFMTGHAEGQGEDMTSN
                     HVHYNNELIRNHCKANNRWLFDFADIEAHDPDGTYYWNQGMEDNLDYDGGNWAVQWCS
                     ANEGSELEQLTTGKGVNGYSGCQGCAHSDSPQQANLNCVLKGRAAWWLFARLAGWDGT
                     P"
     gene            complement(9286..11280)
                     /locus_tag="PK710_02880"
     CDS             complement(9286..11280)
                     /locus_tag="PK710_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08683.1"
                     /translation="MRRLVFALWIALAACSSETEDKESTGTTTPGESEDLGLVSVQPS
                     LLVPGTRVVIRGKAFVPPNIGPSRLVLRGSLDEAPMELVLAAAYVSDTELEASWRGAL
                     ESGLSVKEGVFQGTAVVATDQDDGKTHISPTLSVRLQIAPSSKPDVFDVAQGVIFVND
                     RIEVRGEGFLLGGEEGNSVAMLDGCFRQEGQGSCKPIPTAHVIASPTQPNDRTRIAFP
                     FAPNVAGIHPGSFEGSVVISNEHGLAADSITYKTDSYPVRFDLTSTTIFNLTPKTASL
                     GQYVDIEGGGFVGVPPDGSDPGLALTTLMLSGEFKTGAANPIPVDVTLVPEFVNGNLV
                     RYVLSEKDDELGQAIDLRKTTGDFSGTIQPVIQYEQDSVVGAAHHTSFRIESVKQVVW
                     VNFVASYVGSLRHFGLRAVDPMIRERVLEVAARDYAGTNMEFRQEKPADYALFAQVDL
                     SGPDPNGYGLLGYDNTPGKDVGNDRLYDKIGGVNATTQEDGYAGYGGVFVESFFGFSE
                     HPGILAKRLEGANPFFDSIFDPFRPDVGGRPVEAADLSELIIPKLSSGDECPAVDKTD
                     RGMQIACAIWALGSMIGSTMTHEVGHSLGLADPYGSEFHNLGDGHNRLMDSGSFRPFL
                     ERAEILGYGPAVFCEEDYQYLRFVLPHPDPDPAPSRPPCW"
     gene            complement(11301..13415)
                     /locus_tag="PK710_02885"
     CDS             complement(11301..13415)
                     /locus_tag="PK710_02885"
                     /inference="COORDINATES: protein motif:HMM:NF016071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HOT08684.1"
                     /translation="MVSRHLATLFLIPLLVFPAAVGCGSDDDGGSPPQISPLSDKSAF
                     VDKEFTLEIKATDKDGDPLKFGFRSTIPDIGSRADLRQAGNLAVFRWTPIASDIGVHS
                     FDFTVSDGKHTAKETIAIEVTIQAGSSNAPIFRKPLGTGTTLDLTVKKCIDVDIVVED
                     SDSPGVTLAQDDPVIAGATLDQRDGLSGVWNWCPSKEQVTEGDRFALKLSADDGDNPP
                     TVKNYLIVLRKTQKTDCPGQAPVIEHTPEGNISSVVDLTIDARVTDDQGIKYEPLLYY
                     SYDKPSDPPDLAQMTQVTMLAIEGDMKDGVWAADIPNPVVGKPSGTTADVYYVLVAQD
                     NDDKNGDCDHTTQSPSTGTHHIRVTNPGGSGGLGLCKACSADAQCGSDGDLCATLGAA
                     GKTYCFRKCSSDGDCPGDYLCSSPITSVNGATARQCIPKSGQCGSSTSCVDDSWEPND
                     TMAQAYVLPGLSTGETKGLVSCPGSFTANEDWYPIDLSNSGTMALLLDGGDSSDLDMS
                     LVSSSGTVLARSDELGSFEYLESCQPPGTYYVRVYSWQNQKNTYSLVYDFYPESCASC
                     VDDGNEPDDNATQARIVDLQTPPFKSTTNAICPWNEDWYSVYLFAGETLYASLAFEQK
                     GSKDDLDLILYRGNINLTPCDETTVSGCNATNGQSGTSNENLVWSITTAGYYYVVVRG
                     FNGSENLYDICIGLSQSQCPKL"
     gene            complement(13714..15459)
                     /locus_tag="PK710_02890"
     CDS             complement(13714..15459)
                     /locus_tag="PK710_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08685.1"
                     /translation="MMRNTSSFRLFMQPARLVSVALLTTVFGAGVGACSSTDEGASGD
                     DWEDYGIEGAFGEDGPPLGKADSANVQGPWVNTNTTATQVWTARKAWEDKDPAAGLAW
                     PANSGLNWDQKYSAWVDSMERTRSDSYFDTFMLTTPWGKQLPAPKLECAELAMFLRIT
                     FASWYELPFFLTATDGSQRIYFGHFGARTTSEKYKNTPNYAQVYKDYSNLSAAQYQSN
                     WPKDTSLRAKGLYGGGDEMDFVFSGAKAGAYFDEIFLNKRVGHFMLLTLSYFGSMHLA
                     STRNTYNLKPESVKPGDVLLERWQRKGIGHTLVVKEVTSLEMGQLEVNLASGSMPRRQ
                     PKWEDAVASKSYFTNNYCGGTGTNSDGDSYASLGGGLKRWRVVKNINGYWTNTWMKSD
                     EASWISDTDTTAISSRPEQFETLLGESDPAAMRDSLLAMIEDNRNHLRNYPASCAART
                     RREEGFDKLYTLMQNKFGWSKSQVDQKYRILEDYVFAELEYDKSKTCCWNSTTSVMHQ
                     IAVEYNESLMTNQCVEPVVFKWNNGYDVFKQYAEQTGRGFQWKPWSADETCPQQNVQV
                     DTEANHSWVPYCSLD"
     gene            15701..16489
                     /locus_tag="PK710_02895"
     CDS             15701..16489
                     /locus_tag="PK710_02895"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HOT08686.1"
                     /translation="MNGSSPPRTLFFSLPLSALVALAGCGATTVVPPASGESKPTAIQ
                     SESAKPKNDQPSFGAEYGGMNEGAVARAFLSTFDALNECFDDGCRSMPYLSGEVSFYL
                     EVDSSGSVVHGHVEESDLGDRDVSGCMLNVLRKKIWPSPVGGKRGKIRTSMAFSPLPD
                     VTPPTTWDSQQVAAALEQLSEPIARCKDGITDAFTVTAYVGTLRNPDADTGREPAEVG
                     KMMALEVIPPTKEGVAAADCLANVLRSGTYPSPGQRPAKVTFTL"
     gene            complement(16535..17113)
                     /locus_tag="PK710_02900"
     CDS             complement(16535..17113)
                     /locus_tag="PK710_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOT08687.1"
                     /translation="MTANPPNAPCPKKKFPLWILVLLVVVGVMVVFIGILSALAIHGF
                     TKYMKAAKTSEAKATVGMIAKSASAAFERELVANDGNAYHAMCPSASNPVPQDIARVR
                     GVKYQSNLTEWDVDRAMNRGFACLGFSMANPQYYQYDYQASGSPALDRFQAIARGDLN
                     GDGVTSSFVLEGRLANGVLLLSPTIVESSPEE"
     gene            complement(17186..18331)
                     /locus_tag="PK710_02905"
     CDS             complement(17186..18331)
                     /locus_tag="PK710_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HOT08688.1"
                     /translation="MNFETLIDSRRVILCVGSGGVGKTTTAAALGLLAAQRGRRALCL
                     TIDPARRLAQSLGLEEFRADAQQVSAERFRQAGIEAPGSLTVMMLNTKRTFDEIVERY
                     ASSPEVRDRIMHNQMYHYVSTALAGTQEYMAMEKLYAVKNDPNFDLIILDTPPTSNAL
                     DFLDAPDRLIDALDSSATRWLIRAFESTGRLSFDLLARSAAMALRGVGKFTGTGFLEQ
                     VAEFLTELNDLFGGFTQRARQVQSALRAADVAYVLVTSPSPTTIREVLFFSERLTQLG
                     MSSDAFVINRIHHPPPNAPSQQAIRQAVEQSGLALDEDVVTRLQAIMKDEATQAAMDA
                     HHLLVLHEALHLVDPAPLRAMVPAFPYDVHDLESLHDVAKALARVTT"
     gene            18417..18806
                     /locus_tag="PK710_02910"
     CDS             18417..18806
                     /locus_tag="PK710_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08689.1"
                     /translation="MPQTVERRKRRSADPIVALHLQLAAVRTAAELEAVVLVDDMGCV
                     VAGAGAWPICEELAAFTPFLIANTTPCSRSVASQAAKMAPDTHLLPMSIDGMDVVLCA
                     RAPQGKNIEYQMDRAAAGCQRILSVAC"
     gene            18856..19584
                     /locus_tag="PK710_02915"
     CDS             18856..19584
                     /locus_tag="PK710_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HOT08690.1"
                     /translation="MPAYIDLHCHWIAGVDDGVRTIGEGIALLKALSQAGFSHIIATP
                     HMRPGMFDNERRDLEHAYAQMLPHVMQAGLDVQLGLSCEHFFDDVVYGRIMSGQGLAY
                     PGERAVLVEFAGDHLSSALLEGLFDIRRKGMRPVIAHPERYRACFGDLTLMEDWVDRG
                     LVLLMDGGAMVGKYGRAPQKAAERMLEAGLYYAVCSDAHRPEDVAQVVAGLERIRALV
                     GDEEFDFLLKEGPHRVLTGTVDLG"
     gene            19761..>20305
                     /locus_tag="PK710_02920"
     CDS             19761..>20305
                     /locus_tag="PK710_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT08691.1"
                     /translation="MRPCPSCCSSSDEAQRSSETTMGTGDPRQEDSYWKKLFEGALDA
                     VSVLDAHGTVLYESAACERIHGLSLQDRVGSKALSLVREEELGPVRDKLAHLIAQPGV
                     TMTHDFHICLMDGSERLIRASACNLLHDETVSGVVVHWLDITELERATTELLVQKSRY
                     EKIVEMSQEGIVVLDSDGVIE"
BASE COUNT         4479 a         5528 c         5954 g         4344 t
ORIGIN      
        1 ccgcttgcgg ccgtccggag attgttggtc ggatggtgct tgacaagcgc acttacgggt
       61 ggaaccaatg ggcgttgtct agaggaacga ggtgaaaaag ggatgggtac caaggcgaaa
      121 gaaaaggcgg tacactcgtt cggagatgcc cgaacgcacc atttttcacg tggatatgga
      181 tgccttctac gccgcgatcg aacagcggga ccatcctgaa ctgctcggga aaccagtgat
      241 tgtagggggc gtcgcgagtc gtcggggagt cgtatccacc gcgagctatg aagcacgcgt
      301 ctacggagta catagcgcca tgcccatggc gcaagccctt cgaaaatgcc ctcacggtat
      361 cgtcatgccg gtgcgaatgt ctcattatat cgaggtcagc cgtcagatca tggctgttct
      421 tcactcgttc agccctctgg tcgagccgtt gagcctggac gaggcgtttc tcgacatgac
      481 aggaacccaa gccctgcacg gctccgcgga aacgaccgcg aaatcgatca aacagcgcgt
      541 gctcgagaca acatcgttga cgtgttcggt gggcgtcgca gacaacaaat tccttgcgaa
      601 actcgccagc gacttacata agcccaatgg aattaccctg gtgccacctg gcgagggtgc
      661 ctctttcatc gcgccacttc cgattcgacg gttgtgggga gtagggccgc gcacggcgca
      721 gcgattggaa aaaatagggt gtaagaccat cggcgatgtg gcgtcgatcc aatcgggctg
      781 gctggtacgc gtgctgggcg aagcgagcgc agagcatctg caagcgttgg cgagagggca
      841 tgacgacagg cctgtcattc ccgaccgaga agccaaaagc attggttcgg aagaaacgct
      901 cgaccgggat attacggggc agggggctgt gttgctgttg gtgcgacgac attgtgagcg
      961 ggtggctttt cgtttgcgtc aagcggggct gcaagctcgc ggaattcgtg taaaattacg
     1021 atatcaaaag ggttttcgtc taaccacccg ggaaaccacc tgggcgcagc cctgcgatga
     1081 ttccagaacc atgtaccgtg cagcgttggg gttggtgtcc aaactcgacg tcagcgaacc
     1141 gattcgcctc gtgggtgtgg gagccgttca cctcgagcca tcaggcgtca caaagcaagg
     1201 ggatttgttc gagcacggat cgtcgaaaaa agattcgcgc ttggagcata ccctcgatgc
     1261 cattcgtatg cgattcggtg atgtcattcg tcgagcccag gattgacttc gcgctgaggt
     1321 ccgcgtctga gaaacaatcc tgctcaccgg gctctgtcga tgctcacggt cgggcctaga
     1381 cgatatgggc agagccatga tttatgctat cctgagaggt acattttttg ctcggtaggc
     1441 gaagaccatg acgatgaagc agaaactttc ctgggtcgct gttgcggcag tctgtggggg
     1501 agcggtcctt ggtgcatgct cgggcggcaa cagcgatggt agcgcatcga atgcgaatac
     1561 ccatccggat ggtggggcat cggaggcttc ggccgatggc gcgctgccgg tcgatggagc
     1621 cgaagggtcg gtgttcgacg tgacctcctc ggatgcgcac gacatcacga tttatccgaa
     1681 agaccccgtg atcacggtgt cgacgggcca gcctatccca agccttacgt ttgaagcgcg
     1741 ggtcaatgac aagtcggtga cggccctttg gctcgtggat cgcggggaaa tcgggctcat
     1801 tgacgttcac tccggggtat tcgagcccgg gggtgctatt gcgggggttg cgaaggtggt
     1861 tgccacggta gggacggagg acgtctcgac gacggtaacg gtgaagatat cggtggttca
     1921 aaatggaggt acgggtggtt cggggtctgg tggcgtgggt ggggaagggc caggggtgcc
     1981 cgtgaacgca gcgatacaag gaattctcga gggggcaacg acggcagatc cgaattttag
     2041 gtggttgtac ccttacgatg gaacggtgtg gcctcttggg ctattgtcgc ctcttctgca
     2101 gtggacgcaa ggggcggcgc cggcggaggc ggtgtatatt catctttcga gcgagcatta
     2161 cgactataag gggttttttg gacttccacc cgctttgcca cccggtgctc cgctggttcg
     2221 tcatccgatt ccgcaagagg tgtggaaagc agccacgaca tcgaacgcgg gaggcacgtt
     2281 gacggcatcg ttggtcgtcg cggcgggagg cacggcctac ggacctatat cccagacctg
     2341 gaaacttgct tcagggccgc tgaaggggac ggtctattat caatcttacg gcacggctct
     2401 cgccaaaaac tacgggggtg gtatcggcgg ggatgggcaa tttggcggcg caacattggc
     2461 gattcggggg gactcttccg aaccaaccct cgtggcgggc tcgaatggcg ggctcaaaga
     2521 atgccgcgta tgtcactccg tatccgccga cggaagccgg atgatcgtgc aacgagggga
     2581 tgattatgcc gcgagtacct cgttcgcctt gacggcggga tattccgaaa ccatctatcc
     2641 tgtcgcggat cgggaaaagt tcggatgggt gggactcacg ccggatggcg cgttgggtct
     2701 gggtaatgcc gcgcctctat atgctggagc ccctaccagc agccaactat acaatatgaa
     2761 tacgggtgag gtgatcgctt ccacgggatt cgcttctttc gtcacgaaag ctggatttcc
     2821 cgccttttcc ccgtctggag gccacgtggc tttcagccta ttcgaaggcc caggcaacgc
     2881 cgcgaccgga cccggagatg gttcccaact catcgcgatg gccttcgaca gggccaccgc
     2941 caccttttcc gaccccaaac tgctattcca agctaccgcc ggaacagccc cggggtggcc
     3001 gtcgttcttg ccaacgctcg atgccgtcgt gttttccgtg cagtctcgcc tcaacgatgc
     3061 gggcgaattc atgatgaccc gctacggggg aagaggacaa ctgtggtggg ccgacctcgc
     3121 tacaggtacg gcccatcccc tcgaccgggc caacggcagc gatggaacca actcctatct
     3181 gcccaccggg cccaacggcc acgacgacga tacatcgttg cattacgaac ccacggtcaa
     3241 tccggtcgtt tcgggaggat atgcctgggt cgttttcacg agtcgtcgca tgtatggcaa
     3301 tgtagcaacg attaatcctt gggcgagtga cccacgggag catgatctgg tcgcgaacgt
     3361 cacgcccaaa aaactttggg tagcggcgat cgacttgaac gcggcaccgg gcaccgatcc
     3421 cagtcatccg gcgttttact tgccagcgca agaactcatg gctggcaata cgcgaggatt
     3481 ctgggtggtg gacccctgca aaagcgacgg ggaaccttgt gaatcgggag acgaatgttg
     3541 tggtggcttc tgtcaaatgg atggggatgc gggaggactc atctgcatga accattcgaa
     3601 tgaatgctcc aacgaatacg aaaaatgcgc gcaagattcg gattgctgcg actttttgac
     3661 cggaacgaag tgcatcaacg agcgctgtgc gaacgtggtg ccgaagtaaa aaacttgctt
     3721 cgttacgagg gctactctct cgatcaatgc aacgttgcca aagggccttc aaataattca
     3781 acacgcctcg acgatggcat gcgacgcaac gcgatcactt cgtagggctc gcacgcccaa
     3841 gccacttcgg gcgacgatgt cgtgagaatc cattggacgc gaggaagagc gccgcgcaac
     3901 gcggcaacga tgccccgctg gacataggga tcttggtgca gcgcaacctc gtcgatcaac
     3961 acgactcctt cggtcatgcg cgggtcgcgt cctggatagg cagcggcaag ggcgcgcacg
     4021 ggcaagacgg caaaggctac cagatgacga atgctcgtgg gcaaccgatc gaatgacatg
     4081 atctcgcctc ggtcagtggc aaacacgggt tcgagggtga gcgggtggac gccaagatat
     4141 cgcaccgata ccagggctag cagtctcgcg agcaaatcac gcatggcttc gtcgacgacg
     4201 gcgagggaaa gggagagtct tcccgaatcg ggctccggga tacggctggc gatcgctgcg
     4261 tatacgagcg cgagtttgac atctcgggcg atatccgcat ggcttgcgtc atcgaaagac
     4321 tgtgcggctc gcacgtcata gcgaagcacc gttcgctcag gagaggagag agagacggat
     4381 gcacgagaga accatcgtgt ggaagacatg ctcatgacca tgaatccgcc ttgctcgacc
     4441 gctcgtcgat caaagagcgt ttgctcgcgt cttcgtaggc ggtcttcctc cgtatccttc
     4501 accggcgtat gcggtgagca tacttgcaag ggatgagggc gctccggatc gtcatcgctc
     4561 aagtcccaat cagcgatgac atagcctggc gttgctgctt gggaacgagc catggcggca
     4621 acggcgtggg ccggacgtgt cgaggcaatg gcagcgagca acgtggtttt tccaacaccg
     4681 cttcccccga ccacgatggt gacatttcgg gcttgctctt cggggcctcg aaacgaaacc
     4741 acgacatcgg caaaaggacc aagatcgacc acacgcaatt gcaaaagctt catcgggtgg
     4801 ctcggacttt agctggtcta gaaatctttt gcgacagcgg tcgaaccatg tcgaatgtga
     4861 atacgggcag catcgagtat cggcaagtca tagcgagtct gtggcgatga tgctagggag
     4921 gcgttggtca ttgggaccat gacaagtcag tcacggagca tggttgcgtc atgatcagct
     4981 atccgcctcc cccccgcgag cgcaatgtgg aagtgctgcg ggagatagcg agaaaaattc
     5041 gcgtttccat cgttcggatg attcacaaag cgcgttctgg tcatacgggc agttcgctat
     5101 cttgtgcgga tattctcacc tgcttgtatt gcgcggagat gcggcactat ccacttcgac
     5161 cggattggaa agggcgcgat cgattcgtgt tgagcaaagg acacgccgct ccagcgcttt
     5221 acgccgtgct tcatcattgc ggctacatcg ctcagtccga cctcgatcga cttcgatcgc
     5281 tgtcgagcat tcttcaaggg cacccggatt cccgtcgttg tcctggagtc gaggcctcga
     5341 ccggttctct tggacaaggg ttgtccatcg cgcatggtat ggccctcgcg cttcgtggag
     5401 agcaggaaaa cccgcgggtt tacgtgctgc tgggagatgg ggagttgcaa gaaggacaag
     5461 tatgggaagc ggccatgagc gctgcccatt atcgaacctc gaacctttgc gcattcatcg
     5521 acgccaacgg attgcaaatc gatggtcccc tcgaagggat catgggtgtg gaaccgatgg
     5581 tggataagtt cgcggctttt ggttggaata cgatggaaat cgatggtcac gacgtggggt
     5641 cgatcctgtc ggctctcgat agcgcacgaa agcagaccca cgcgccatcg gtcatcatcg
     5701 cacgcaccac gaaaggaaaa ggggtgagtc gtttcgaaaa caaggtgcag tggcatggga
     5761 aggcaccggg agatgacgat ctagccttgg ctctgatgga actgggtgaa ctcgacaagg
     5821 aggcgtcatg aacgggcgag gggtatctcc ccgcatcctg gctacgcgag aggagtatgg
     5881 gcgaacgctc gtgcggcttg gagagcggag ccgcgatatc gtggtgttgg atgcggactt
     5941 atcgaagtcc acgtgtaccc acgagtttgc caaacgattt cctgaccgct ttttcaacat
     6001 ggggatcagt gagcaagacc tgatcgggac ggccgcgggc atggcgctcg ccggcaaaat
     6061 ccctttcgtg agcagcttcg ccatttttct gacgggaaga tgctgggagc aaatccgtca
     6121 aagcgtggcg cttccaggtg tcaacgtgaa ccttgtggga acgcatggcg gtgtcaccgt
     6181 cggaaaggac ggcgcttctc accaggccct cgaagatatc gccgtgatgc gcgcgatccc
     6241 ctcgatgacg gtcattgttc ccgcggatgc cgtggaaacg cgcaaagcgg tggaggcggc
     6301 ggcgtggtac gacggccccg tgtacattcg actcacgcgg gaaaagctcc cagtcgtcta
     6361 tcctgatgaa aaaccttttc gcattggcgc gggagcgatc gtgcgcgagg gcagcgacgt
     6421 gaccttatgc gcgatcggcc tcatgctcca cgtggcgctc gaagccgccg aacggcttgc
     6481 catggaaagc atttccgctc gcgtggtcga cttggccacc gtaaaaccga tcgatgagtt
     6541 tttggtgacg aagtgcgcac aagagacggg gtgcatcgtg accgcggaag aacacagcac
     6601 catcggaggt ctgggggatg cggtaggcga agtgctgttg cgacgtcgtc cggttcccat
     6661 ggaacgtgtt ggcgttcgcg atgcattcgg ccggtcgggc agtgctcatg aactcctcga
     6721 gtatttcgga ttgaccccgc aggccgtata tgaagcggcc aaacgggcca tcgagcgcaa
     6781 ggcagctcac taagctattt tcttcgcgaa aggtgcgaca tggtttctct caaactattt
     6841 accggcagtt ccaaccccga actggcccat aaaatcgccg cggaattgga gatcgacgtg
     6901 ggcgatgcgg atgtcacgcg cttctccgat ggcgaagtcg atgtcagaat caacgagagc
     6961 gtgcgcgctt gtgacgtttt catcattcaa tccacatcct atccagtcaa cgatcacttg
     7021 atggagttga tgcttctggc ggacgtgtgt cgcagggcgt cggctgccac catcaccgcc
     7081 gtattacctt atttcggtta tggaaggcag gatcgacaaa cgcaaaaacg ggttccgatc
     7141 tccgcgaaag tcgtggccaa tatgcttacg atcggagggg tagaccgtat catcacggtg
     7201 gatcttcacg ccgaacagat tcaaggattt ttcgatgtgc cggtcgatgt gctgcgttcg
     7261 gcacggttgc tcgtcgataa cgttcatcaa cgcggactgc gagatctcgt cgtcgtcgct
     7321 cccgatgcgg gaggcgtcga aattgccggg cgttttgcaa tgggattggg ggcaacgatg
     7381 gcgctgatcg atcggcatcg cgatgcaccc gccaaagtca aagccatgcg actcatcggt
     7441 gatgtcgaag ggaaagattg cttgctcgtg gatgacatga ttgatacggg aagcactctc
     7501 accgaggcag ccgatcttct catcacgagt ggagccaagt ccgttcgagc cgcggccgtc
     7561 caccccgtgt tgtcaggttc cggggtggaa agactgcatc gatcccgaat cgacgaactc
     7621 atcacgaccg ataccatccc attgccagtc gaaaagcaga ttgagaaggt cacggtcgta
     7681 tcgatggctt ctctgcttgc caaagccatt cggcacgttc acgtgggaaa gtcactgtcg
     7741 aacgtggagt agtcgaacga tgcatgggaa ttgatgcgcg ggattttcgg tgggtgtagt
     7801 cgagatcaca ggcgaattca gggtgttccg tcccagcccg cgagtcgggc aaaaagccac
     7861 caagccgcac gacctttcag aacacaattc aagttcgctt gttgtgggct atccgaatgg
     7921 gcgcagcctt ggcaaccgct atatccgttc actcccttgc ccgtggtcaa ttgctcgagt
     7981 tcacttccct cgttggccga acaccattga accgcccaat tccctccgtc gtaatcgagg
     8041 ttgtcttcca ttccctggtt ccaatagtag gtaccatccg gatcgtgcgc ttcgatgtcg
     8101 gcgaagtcga agagccatcg gttgttggcc ttgcaatgat tgcgaatgag ttcattgttg
     8161 taatgcacat ggttcgaagt catgtcctcg ccctggcctt cggcatgacc ggtcatgaaa
     8221 atgaaatcga cggcgggata ctccttgacc agtttttcca tattgtcgac atatcgctgc
     8281 gcatcgtgac cattgatgct gcaccaagac cacatcacca cattgatatg gctgttggat
     8341 gatttattga gcaaatcgcg tgtggcgatg acccaagggg tatccccatg actgtccacc
     8401 gagtcccctt ggctgagatc cgcacaacct gggataccat tgtcatccag atcgagagca
     8461 ttgggagctt ggccggcatc gtcccaagca taggtgttac caaaagacgg aaaagcctcc
     8521 aaggcattca ttcccgtgat cagttggcta ccatgggacg tgtgttggta ggcgatatga
     8581 agctttgatt tggcggcgga aatggcgctg gagggaatca gatcgaggcg tgtgttttca
     8641 tggccgatga cccgtcccgc aggaggcgct ccaccgcatt gacctttcag acacgtttgg
     8701 gatccgccgc acggagttcc gtcgggaagg gctgtgtatt gcgacgtcgt ctttgctgga
     8761 tcacaggcta cgcagggatt cgatgggttg gttgcgccct gctgcacgca agcgtgctga
     8821 atgacacacc atccttgctc gatcgtatgc tcgcaaacgt cgttcacaca ggcatcgctg
     8881 gtacacgcca ggccatcgtc gcagccctgt tcgcaagaca cacttccccc ggctcctgac
     8941 gtattccctc ccgagccacc ttccgtgctg gagccgccgg tgcttccgct tgcaccgccc
     9001 gtgtggcccg cggttccacc ggtacctccg gtgcttcccc cggagcctcc cacgcttcct
     9061 ccggcgcccg ctggaccacc aggtttcgtg ttcccttctt cctcggaatc actgcacccg
     9121 atcgcccaca gcattgcaac ccccagaacc cagcaaatac gtgagtattt catcagcggc
     9181 tccttgactc atgaatggat attccaagtt cgaatacacg ccagcgatag tagagtagca
     9241 gaaaaacgag aggtgcttgc caaaaatgga gcctggaaag ggaattcacc agcaaggtgg
     9301 gcgagagggg gcaggatccg ggtcgggatg gggcaacacg aatctcaggt attgataatc
     9361 ctcttcacaa aatacggcag ggccatagcc gagaatctcg gcgcgctcga ggaaaggacg
     9421 aaagcttccg ctatccatga gacgattatg cccgtcgcca aggttgtgaa actcgcttcc
     9481 atacgggtcg gcgagcccca acgagtgtcc gacctcgtgg gtcatcgtag agccaatcat
     9541 ggaacccaac gcccatatcg cacaggcgat ttgcattcca cggtccgttt tgtccacggc
     9601 agggcattca tcgccggaag aaagcttggg aatgatgagt tccgaaaggt ccgcggcttc
     9661 cacgggtcgc ccccccacat caggacgaaa aggatcaaaa atggagtcga agaagggatt
     9721 ggcgccctcc aaacgtttgg ccaagatacc gggatgctcg ctgaagccaa aaaaagactc
     9781 gacaaagact ccaccataac ccgcatagcc atcttcttgt gtcgttgcgt tgacaccacc
     9841 gattttgtcg taaagacgat cgttgcccac atctttgcct ggcgtgttgt catagccgag
     9901 aaggccatag ccgttggggt cggggccact gaggtcgact tgtgcaaata gagcataatc
     9961 agcgggtttt tcttgtcgga attccatgtt ggtcccggcg tagtcccgcg cggctacctc
    10021 caacacgcgc tcacgaatca tggggtcgac cgctcgcagc ccgaagtgac gaaggcttcc
    10081 tacgtaagaa gcaacgaaat tgacccagac gacctgcttg accgactcga tgcgaaagct
    10141 ggtgtgatgg gcggccccaa cgacactgtc ttgctcgtat tgaatcaccg gttgaatcgt
    10201 tcccgaaaag tctcctgtcg tttttcgcaa atcaatcgct tggccgagtt catcatcctt
    10261 ctcgctgagg acatagcgca cgagattgcc attcacgaac tcaggcacga gggtgacgtc
    10321 gacagggatt gggttcgccg cgcccgtctt gaattctccg gaaagcatga gcgtggtaag
    10381 agcgagcccc ggatcgctgc cgtccggggg aactcccacg aatccaccgc cttcaatatc
    10441 gacatattgg cctaaactcg cggtcttggg ggtgagattg aaaatcgtgg tgctggtaag
    10501 atcgaaacgt acgggatagg aatccgtttt ataggtgatg gaatccgccg cgagcccgtg
    10561 ctcattgctg atgacgacag acccttcgaa ggagccggga tggattccag cgacattggg
    10621 ggcaaaagga aaagcaatgc gcgtgcggtc gttgggttgg gtgggcgaag ctatcacgtg
    10681 ggctgtcgga atcggtttgc atgacccctg accctcttgc cgaaaacaac cgtcgagcat
    10741 ggccaccgaa ttgccttctt cgcctcctaa caaaaaacct tcgccacgaa cttcgatccg
    10801 atcattgacg aaaatcacgc cttgagcgac atcgaagacg tccggtttgg acgagggcgc
    10861 gatctgcaaa cgcaccgata acgtcgggga tatatgcgtt ttgccatcat cctggtctgt
    10921 cgccacgacc gccgttcctt gaaaaacccc ttctttgacc gataagccag attcgagcgc
    10981 cccacgccac gatgcctcga gttcggtgtc cgagacatac gcggcagcga gcaccaactc
    11041 catgggggct tcgtcaaggc taccccgaag aaccaaccga gaagggccga tattgggggg
    11101 aacgaaagcc tttccccgaa tgacgacacg tgtgccaggc actagcaacg aaggctgaac
    11161 cgagactagc ccaaggtctt ccgattctcc gggggtcgtc gttccggtgg attccttgtc
    11221 ttccgtctcc gaagagcaag ccgccaacgc gatccacaat gcaaagacca gccgacgcat
    11281 gattcctccg cgactcccgt ttacagtttg gggcattggg attggctgag accgatgcag
    11341 atatcatata gattctcgga cccattgaat ccgcgtacga ccacataata gtaccccgcc
    11401 gtcgtgatgg accatacgag gttctcgttc gacgtgccgc tttgaccatt ggttgcattg
    11461 catcccgaca cggtcgtttc gtcacaaggc gtgaggttga tatttccgcg atacagaatg
    11521 agatcgaggt cgtctttcga acccttttgc tcgaatgcca gagaggcata aagggtttca
    11581 ccagcgaata ggtagacgga ataccagtct tcattccaag ggcagatggc attggtcgtc
    11641 gacttgaagg gaggcgtctg caggtccacg atccgcgctt gcgttgcatt atcgtcaggc
    11701 tcgttgccat catcgacgca agaagcacag gattccggat aaaagtcgta cacgagcgaa
    11761 taggtatttt tctggttttg ccagctatag actcgtacat agtacgttcc gggaggctgg
    11821 caggattcca gatactcgaa agaaccgagt tcatccgaac gggccagcac cgtgccgcta
    11881 gaactcacaa ggctcatatc caagtcggaa ctgtctcccc catcgagcaa taacgccatc
    11941 gttccagagt tcgaaagatc gatcggatac caatcctcat tcgccgtgaa ggagccggga
    12001 cagctcacta atcccttggt ttccccggta gaaaggccgg gaagcacata cgcctgggcc
    12061 atcgtatcgt tgggttccca agaatcgtct acgcaactcg tgctgctacc acattgacca
    12121 gatttgggaa tgcattggcg agccgtggcc ccgttgacag aagtaatcgg cgatgagcaa
    12181 aggtagtccc ccggacagtc cccgtccgaa gagcatttgc ggaaacaata ggtttttcca
    12241 gcagctccaa gcgtcgcgca caaatcaccg tcgctaccac attgcgcgtc cgcggaacat
    12301 gccttgcaaa gtcccaatcc tcccgaacca ccgggattcg tcacccgaat atgatgggtg
    12361 cccgtcgaag gactctgcgt cgtatgatca cagtccccat ttttgtcgtc gttgtcttga
    12421 gcaaccaaaa cataatacac atccgcggtt gtccccgaag gtttgcccac cacgggattg
    12481 ggaatatccg cggcccaaac gccatctttc atgtcccctt cgatggccag catcgttact
    12541 tgcgtcatct gcgccaaatc aggaggatcc gatggcttgt cgtacgaata gtacagcaat
    12601 ggttcgtact tgatcccctg gtcgtcagta acgcgtgcat cgatcgtcag atcgaccacc
    12661 gaagagatat tgccttccgg cgtatgctcg atgaccggtg cctgtcccgg acaatcggtt
    12721 ttttgtgttt ttcgaaggac gatcaggtag tttttcaccg tgggagggtt gtccccatca
    12781 tcggccgaca gcttgagggc aaaacgatct ccttcggtga cctgctcttt cgatggacac
    12841 caattccata cgccactgag cccgtcacgc tgatcgaggg tggctcccgc aatcaccgga
    12901 tcgtcttggg ccagcgtgac tcccggagaa tcagagtcct ccaccacgat atcgacgtca
    12961 atacactttt ttaccgtaag gtcgagcgtg gttcccgttc ccaaaggttt tcgaaatatg
    13021 ggagcattgc tcgaacccgc ctgaatcgtc acctcgatgg caatcgtttc tttcgccgta
    13081 tgtttgccat ccgagaccgt gaagtcgaaa gaatgcacgc cgatgtccga agcgatgggc
    13141 gtccaacgaa acaccgcgag gttgcctgct tgacgcagat ccgcccggga gccgatatcc
    13201 gggatcgtgg atcgaaaacc gaacttcaaa gggtccccat ctttgtccgt agctttgatt
    13261 tcgagtgtga attccttgtc cacgaaagca gatttatcgc tcaacggaga aatttggggt
    13321 ggacttcccc catcgtcatc cgaaccgcag ccaaccgctg cagggaagac aagcagggga
    13381 atcaaaaaaa gcgtggcaag gtgacgcgaa accatcgaag aacctttcga tagaaaaaga
    13441 acgaatggtt gaatgatacc gcggacagac ccattcgtca tgcaaagcga gccttttgca
    13501 gcaagttgct agcgtgcaac tttgccgtga ccctgccgcg ctggttcgag accgtggctt
    13561 tgcgacgcca cgaatgaaac gctctgccat gggagaccaa cggtttcgaa agatcatcgc
    13621 ttaccgaaag gtcggccagt ggagcatgcg aattgagtag gtgcgggtag gggaagcgaa
    13681 cggatggcgg cgagcggacc agattccccg aggtcaatca aggctgcaat aaggaaccca
    13741 gctatgattg gcctccgtgt ctacttgcac attctgttgt gggcaggttt catccgcgga
    13801 ccacggtttc cattggaaac ctcggcctgt ctgctcagcg tattgcttga acacatcgta
    13861 accgttgttc cacttgaaaa cgacgggctc cacgcattgg ttggtcatga gcgactcgtt
    13921 gtactcgaca gcaatttggt gcatcacgct ggtcgtggaa ttccaacagc aggttttgga
    13981 cttgtcgtat tcgagttccg cgaagacata gtcttccaaa atccgatatt tttggtcgac
    14041 ttgtgatttg ctccatccaa atttgttttg catgagcgtg tacagtttat cgaagccttc
    14101 ttccctacga gtgcgcgcgg cgcaagacgc tgggtaattg cgaaggtggt ttcggttgtc
    14161 ttcgatcatc gcgagcaagg aatcacgcat ggccgcagga tcggactctc ctagcaacgt
    14221 ttcgaattgt tctgggcgag acgagatggc cgtcgtatcc gtatccgaaa tccaagaggc
    14281 ctcatcagac ttcatccacg tgttggtcca ataaccgttg atgttcttga ccacacgcca
    14341 ccgtttgagc ccgccgccga gagaggcata tgagtcgcca tcggaattgg tgccggtccc
    14401 accgcagtaa ttgttggtga agtagctttt cgaagcgacc gcatcttccc acttcggttg
    14461 acgacgaggc atcgagcccg aggccagatt gacttcgagt tgtcccatct cgaggctcgt
    14521 tacctctttg acgaccaacg tatggccaat accttttcgt tgccaacgct cgagcaatac
    14581 atcacccggt ttgaccgatt ctggcttgag gttgtaggtg ttgcgcgtac tcgccaagtg
    14641 catcgagccg aagtacgaaa gcgtcaacaa catgaagtgg ccgacacgct tgttgagaaa
    14701 gatctcgtcg aagtacgccc ccgctttcgc tcccgagaac acgaagtcca tctcatcgcc
    14761 gcctccgtac aagcccttgg cccgcaacga tgtgtccttc ggccaattgg attggtattg
    14821 agcagccgag aggttcgaat agtccttgta aacctgcgcg tagttcggcg tgttcttgta
    14881 cttttccgac gtggtccgcg ccccgaaatg cccaaagtaa atacgctgag agccgtccgt
    14941 ggccgtcaaa aagaaaggca gctcatacca ggaagcaaag gtaatgcgca aaaacatcgc
    15001 aagctccgca cactcgagct tgggcgcggg aagctgcttg ccccaaggcg tcgtcagcat
    15061 gaaagtatcg aaatacgaat cactgcgcgt acgctccatc gaatcgaccc acgcggaata
    15121 cttctgatcc cagttcaatc cactgttggc cggccaagca agccctgccg cgggatcctt
    15181 gtcctcccac gccttgcgcg ccgtccacac ctgcgtcgcc gtggtgttgg tgttgaccca
    15241 cggtccctgc acgttcgcac tatccgcttt gccaagaggt ggaccatcct ccccaaacgc
    15301 cccctcaata ccgtaatcct cccaatcgtc gcccgatgcc ccttcgtcgg tactggaaca
    15361 agcccccacg ccagccccaa acacggtagt aagcaaagcc acggacacca atcgcgccgg
    15421 ttgcatgaac agtcgaaacg atgaagtatt gcgcatcatt acaaaacctc ccagatcaac
    15481 acgtcatacc agcgcgcgga accgcagaag tcaaggcaag aaccaagaaa ataaaaagat
    15541 acacggataa cggaatccaa atccgttctg atcggtgtaa cacattgaaa ttacatcatt
    15601 tgacgaaaca agagggaaag actcgtcggc actgtctttg cgaagcatcc cagggctcga
    15661 gttgcttcaa agaaacggaa aagagagtat acaccttgcc atgaatggtt cgtcgccccc
    15721 tcggacgttg tttttttcgc tacccctgtc cgcgctcgtt gcgctcgcgg gatgcggcgc
    15781 cacaacggtg gttcccccgg cgtccggaga gtccaagcct acggccatcc aaagcgaaag
    15841 tgcgaagccg aaaaacgacc aaccatcctt cggagccgag tacggcggca tgaacgaagg
    15901 cgccgtggcc agagcgtttc tttcgacgtt cgatgccctg aatgaatgct tcgatgacgg
    15961 gtgtcggtcc atgccctacc tttcgggtga agtgtctttc tatctcgaag tggatagctc
    16021 tggctcagtg gttcacggcc atgtcgaaga atcagacctc ggagatcgag acgtttctgg
    16081 ttgtatgctg aacgtgcttc gaaaaaaaat atggccttcg ccggtcggag gcaaacgcgg
    16141 caagattcgg acgagcatgg ccttttctcc gttgccggat gtcacaccac cgacgacatg
    16201 ggattcccaa caggtggcgg cagccttgga acagctttct gaacccatcg ctcggtgcaa
    16261 agacgggatc accgatgctt tcacggttac cgcctatgtc ggaacgcttc ggaatcccga
    16321 tgcagacacc ggccgagagc ctgccgaagt cggaaaaatg atggccctcg aagtgattcc
    16381 ccccaccaag gaaggcgtcg ccgccgcaga ctgccttgcc aacgtgttgc ggtcgggaac
    16441 gtatccctct ccaggacaac gacccgcgaa ggtaaccttc acgttgtagg tttcgtgcgg
    16501 gatcgccttc gacacccgcc gaatgatttt cgactcattc ctcgggtgag gattccacga
    16561 tggtgggaga gagaagtagc actccgttgg ccaatcgtcc ttcgagaacg aaactggaag
    16621 tcacaccatc accattgagg tcaccccgcg caatcgcttg aaaacgatcg agggccgggc
    16681 tgcccgaagc ctgataatcg tattgatagt actgaggatt ggccatcgaa aatcccagac
    16741 aggcaaatcc acgattcata gcacgatcca cgtcccattc ggtcaagttc gattggtact
    16801 tgaccccgcg tactcgtgcg atgtcctgcg gtacaggatt cgatgccgaa ggacacatcg
    16861 cgtggtaggc gtttccgtcg ttggccacaa gctcacgctc gaaagccgcg gaggcgcttt
    16921 tggcaatcat tcccactgtt gccttggcct cggacgtttt cgcagctttc atgtacttcg
    16981 tgaaaccatg gatggcgagc gccgaaagaa tgccgatgaa gaccaccatc accccaacaa
    17041 caaccagaag aacgagtatc caaagtggaa actttttttt cgggcaagga gcgttgggag
    17101 gattggccgt catggtttgg gtatattcga gagcgtcaca cagtgtcgac cacaatcgca
    17161 gtaagcatgg gatgatccga tcgaatcacg tcgtaacgcg cgcaagcgcc ttggcgacgt
    17221 catgcaacga ttcgagatca tggacatcgt aagggaacgc tgggaccatc gcacgcaaag
    17281 gcgcgggatc gacgaggtgc aatgcctcat gaaggacaag caggtggtga gcatccatcg
    17341 ccgcttgcgt cgcttcgtcc ttcatgatgg cctgcaaccg cgtgaccacg tcttcgtcga
    17401 gggcgagtcc ggattgttcg acggcttggc gaatggcttg ttgcgatggt gcgtttgggg
    17461 gtgggtgatg aattcgattg atcacgaacg cgtcgctcga catcccgagt tgggtaagcc
    17521 gttccgaaaa aaagagaacc tcccggatgg tcgtgggcga tggggaggta acgagcacat
    17581 acgccacgtc agccgcgcga agggcacttt gtacttgccg ggctcgctgg gtgaagccac
    17641 cgaagagatc gttgagttcc gtaagaaatt cagctacttg ctcaaggaat cccgtgccgg
    17701 tgaacttgcc gacaccgcga agagccatgg ccgccgaacg cgcgagcaga tcgaacgaaa
    17761 ggcgccccgt cgattcaaag gcgcgtatga gccatcgggt cgcggaactg tcgagggcat
    17821 cgatgagccg atcgggagca tcgagaaagt cgagagcgtt ggaggtgggt ggagtatcga
    17881 ggatgatgag atcgaagttg ggatcgtttt ttaccgcgta caatttttcc atggccatgt
    17941 actcttgcgt gccggcaagt gccgtggaca cgtagtggta catctggttg tgcatgatgc
    18001 ggtcgcgtac ttcagggctg gatgcgtagc gttcgacgat ctcatcgaac gtccgtttgg
    18061 tgttgagcat catgacggtc aacgagccgg gggcttcgat accggcttgc ctgaaacgct
    18121 cggcagagac ttgttgcgcg tcggcacgaa actcctccag accgaggctt tgggcgagcc
    18181 tgcgtgccgg gtcgatcgtc aggcataggg cgcgacggcc tctttgagcg gccaaaagcc
    18241 ccaaggcggc ggcggtggtc gtcttgccaa caccgccgga gccgacacag aggatgacac
    18301 ggcgagaatc gatgagtgtt tcgaagttca caatgggaaa cccgtcaaca tgtcacgaac
    18361 ttgaccaagg agggcctgac acgcgatcct ttcgttcgca accaggagaa acctcgatgc
    18421 ctcagactgt ggaacgacgc aagcgtcgga gtgcagaccc gatcgtcgcc cttcatcttc
    18481 aattggcggc cgtgcgaacg gctgcagagt tggaagcggt tgtcctcgtg gacgacatgg
    18541 gatgtgtcgt cgccggcgcc ggggcgtggc cgatttgcga agaactcgca gcttttaccc
    18601 cgtttttgat tgccaacacg actccctgca gccgctcggt ggcttcgcaa gccgccaaaa
    18661 tggcgccgga tacccatctg ctgccgatgt ccatcgacgg gatggatgtg gtgttatgcg
    18721 cccgcgcgcc acaaggaaaa aacattgaat atcaaatgga tagggcagcc gcgggatgcc
    18781 aacggatcct gtcggtggct tgctgaaaaa aaacacccct ttcggttgtc gtcgtcagag
    18841 acataaatac gcgcgatgcc tgcgtatatc gatctgcatt gtcattggat agccggagtg
    18901 gatgatggtg tgcgaacgat cggtgaggga attgccctgt tgaaggctct gagccaagcc
    18961 ggtttttctc atatcatcgc cacgccccat atgcgccccg gcatgttcga taacgaacgt
    19021 cgggacttgg aacatgccta cgcccaaatg ctgccgcatg tgatgcaggc gggcctcgat
    19081 gtgcagttgg gattgtcgtg cgagcatttt ttcgacgatg tggtgtacgg gcgaatcatg
    19141 tcggggcaag ggcttgccta ccccggtgag cgtgcggtgc tggtggagtt tgcgggtgac
    19201 catttgtcga gcgcgctatt ggaagggctt ttcgacatac gtcgcaaagg aatgcggccg
    19261 gtcattgcgc atccagaacg ctatcgggcg tgcttcgggg acttgacctt gatggaagat
    19321 tgggtggatc gggggctcgt gctgctcatg gacggtggtg ccatggtggg caagtatggt
    19381 cgagcgcccc aaaaagccgc ggaaagaatg ctggaggccg ggttgtatta tgctgtgtgt
    19441 tcggatgcgc accgtcccga ggacgtggcg caagtcgtag cgggcctgga gcggattcgt
    19501 gccttggtgg gggacgagga attcgatttt ctcctgaaag aagggccgca ccgggtgctg
    19561 acgggaaccg tggacctcgg gtgaaaagct cgaaggagaa gggagtgcga accctcgtca
    19621 tgacgttcga gcaccacggc tggcagaccg aacgtatgaa cttcgtgcgg gtacccccta
    19681 ggcatgctgg accgccgaca ctagactgtc tttgactcgc gcttctattt gtcgtctggc
    19741 gtgatcgctc atttcgaacc atgcgtccgt gcccatcctg ttgttcatcg tccgacgaag
    19801 cgcaacgatc gtctgaaacg acgatgggga ccggagatcc caggcaagaa gattcgtatt
    19861 ggaagaagtt gttcgagggg gcgttggatg cggtttccgt gctcgatgcg catgggacgg
    19921 tattgtatga aagcgcggca tgtgagcgta tccacgggct gagccttcaa gatcgagtcg
    19981 gttcaaaagc tttgtctctc gtgcgtgaag aagaacttgg ccctgtcaga gacaagttgg
    20041 cgcacctgat cgcgcagcca ggtgtgacca tgacccatga tttccatatt tgcctcatgg
    20101 atggcagcga acggctgatt cgagcttcgg cctgcaatct gttgcacgat gaaacggtat
    20161 ccggcgtggt cgtccattgg ctcgatatca ccgaactcga acgagctacc acggaacttc
    20221 tcgtccagaa atcccgctat gaaaagatcg tcgagatgtc ccaagagggc attgtcgtgc
    20281 tggattccga tggtgtgatc gagtt
//
LOCUS       DAOSOR010000030        19736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_2744_length_19736_cov_5.280982, whole genome shotgun sequence.
ACCESSION   DAOSOR010000030 DAOSOR010000000
VERSION     DAOSOR010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 19736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19736
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2744_length_19736_cov_5.280982"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..567
                     /locus_tag="PK710_02925"
     CDS             73..567
                     /locus_tag="PK710_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOT08692.1"
                     /translation="MPDGLAPRAASLWRIRGRDTDTRVEYLGFCLGEDRYALAVDRTG
                     EILRIPPMTRVPRSSDVVMGIVGVRGRVLTVLDVRARLGLPTPAPTRHARIVVVPWSE
                     NESVGLYVDRVVQVYRMTAAEIESAAAGLGRNAGDHVMGIARVDGHLIVVLRLEPFLA
                     LMGT"
     gene            564..1082
                     /locus_tag="PK710_02930"
     CDS             564..1082
                     /locus_tag="PK710_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOT08693.1"
                     /translation="MKHRTHHIRAGGQRSLVGFMVGDVNYAIDVQHVVQVVNPMGITP
                     LPDTPPSIVGVTEHRGLVAPIVDLRLMFGMHPEATRKTKWILVEVGNRVTGVVVDAVT
                     GVFTVSSERIQPAPELGPGDVRRPFSGMTSTEGRLVFVLDLAKLHEGFANIVDMQTST
                     RSEPISVKREGT"
     gene            1079..1978
                     /locus_tag="PK710_02935"
     CDS             1079..1978
                     /locus_tag="PK710_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOT08694.1"
                     /translation="MISHGSGRSLPTELPELRPEEYRLLRDLLGEKIGHAFNEDHARE
                     FERRLRPRVSACGLSTFAEYHRHLRGSPKREEELQTVYDILTNNETYFFREDYQLRSF
                     REEVLPRLERDARHRKQLTCWSMGCSTGEEAYSIAMVLLESGRFDGWSMRVLGTDLSR
                     KRITFARRGIYGESSFRSTSKERRGAFFVSTRAGTRVCAAVRSLCQFGQLNVMEMKNR
                     CTIDQVDAVFCRNVLIYFTPQARAHVMASIFERLRPGGFLMLGHSESLLHDPGPFQPV
                     HLKRDVVYRRPTERELRSKREGI"
     gene            1975..3036
                     /gene="cheB"
                     /locus_tag="PK710_02940"
     CDS             1975..3036
                     /gene="cheB"
                     /locus_tag="PK710_02940"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HOT08695.1"
                     /translation="MMDAHDDRVRVLIVDDSTLNRRGIRRVLESSGCVQVVGEASDGD
                     EALRMVTNREPEAITLDLEMPRLDGFSFLRILMARKPLPVIVVSSHSQKENVFRALEL
                     GAIDFVEKPEHGFFDEQAAQQLVDKVLLARTLRPVQAHTPPIFASEPDTARGLGTVAT
                     TPLPPRFVVAIAASTGGPTALLDVFSRLPSRISAAILVAQHMPPRFTGAFAARLDRKS
                     ALTVREASDGEALLARHGYVCPGGKCMEVEANQGEMRLRVQPPNRNDGYVPSADRLFK
                     SVAQIAKSRAIAVVLTGMGTDGVQGAKAIRDGRGLVIVESAETAVVDGMPSAVIRAGV
                     ASRVLPLPAIGELLATLTG"
     gene            complement(3176..3736)
                     /locus_tag="PK710_02945"
     CDS             complement(3176..3736)
                     /locus_tag="PK710_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08696.1"
                     /translation="MNGFLVSSRAIASSLLWLALSIMACSATPPAHWAQGGMPIALGQ
                     AYWERDSDLIEILPDGTITENGKERLLIDQVGRVYYKNGDPYALLLPNGHLVGEDNIG
                     MGEVGPVSASFPGSAFAWMAIDPAGQLIRYDQDGNEYHDGKWSGCQGPMSRTCMLVSH
                     VIALGEWQRRPRVGVGVGVGVMMPLN"
     gene            3829..4200
                     /gene="queD"
                     /locus_tag="PK710_02950"
     CDS             3829..4200
                     /gene="queD"
                     /locus_tag="PK710_02950"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOT08697.1"
                     /translation="MNVRLVKEYRFEAAHRLTRVSDEHPCGRLHGHSYRVELSLQGPV
                     DPRVGWLIDFGRIDEAWKPVKSRLDHAILNELPGLENPTSENLAAWIWDALIDALPML
                     CRVTLWETADARCEFEGHGAR"
     gene            4399..4998
                     /locus_tag="PK710_02955"
     CDS             4399..4998
                     /locus_tag="PK710_02955"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HOT08698.1"
                     /translation="MAVEFKNFPLEGKLKGISDRQLQEHRDTLYKGYVNQYNRISELI
                     TKGDSSTANPHFSEWGELKRRETWAHNGALLHQMYFENLTEANSTKPGTKTTKLIERD
                     YGSLEAFKADLLNAGKVAPVGWAVWAYSVLDKKTHVFLIEQHMNHTPIGVIPLLILDV
                     FEHAYMIDYGTARPPYLEAFWENLNWNTVEERVAMVPGL"
     gene            complement(5006..5326)
                     /locus_tag="PK710_02960"
     CDS             complement(5006..5326)
                     /locus_tag="PK710_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08699.1"
                     /translation="MPARRNQKLNCWEFIKCGREPGGIHVAQLGVCPAAITVEADCIH
                     GGKNAGRMCWAVAGTMCDGQIAGTFASKMGNCLSCDFYGAVLREEGEQFVSIARILSQ
                     IASE"
     gene            complement(5421..6212)
                     /locus_tag="PK710_02965"
     CDS             complement(5421..6212)
                     /locus_tag="PK710_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08700.1"
                     /translation="MRKRLLVGTLALLGLMVGCSNDDDENGGSPSSSHGGSGGKDAGA
                     QGGSGGSEQGGSGGSEQGGSGGGAQGGSGGSAQGGSGGSAQGGSGGSTQGGSGGSAQG
                     GSGGSAQGGSGGDPSDPFAMEREACFNKVNALRATKGLPPYARWTAAESCSDDEASKD
                     AASGQAHGAFGQCGEMGQNECPGWGASSVEACLESMWAEKDQSGCQGCDACADQYSPN
                     CPGCDFYGSSTGQVCGHYVNMSAKYLSKVACGFSSQGGWIVINFQ"
     gene            complement(6421..7350)
                     /locus_tag="PK710_02970"
     CDS             complement(6421..7350)
                     /locus_tag="PK710_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT08701.1"
                     /translation="MMRRLVWVVWLALLSTGCIATQFLSRNLAAFGQLRTVAHKIENP
                     VRPEARLAVLWVGHATVLIQIDDKILLTDPVWTRTVGQISPRLVEPGIEVRHLPPLDA
                     VLISHMHFDHLSLGSLEMIEDKVRWLGMPEGGLVYLTDFSFPAHEVPWWQTRVLPGDL
                     KVTAVAVRHNGWRYGLDHAWREGIGYAAWVIEYHGITVYFGGDSALAEDAYRETARRF
                     PTIDLALLPIAPIHPRGFMKRVHTDPWEALTAFEKLGATWMVPIHFDTFINSLDEPGE
                     PTRVLEQAVKQNRIAENRVHRLAIGEQKVLISR"
     gene            complement(7350..7985)
                     /locus_tag="PK710_02975"
     CDS             complement(7350..7985)
                     /locus_tag="PK710_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08702.1"
                     /translation="MPYRVIMMDGSFDSDDGVCRTPRCCRGKVPISVVLGLVFFLFCH
                     DALAQSDGGAHPRPSWPWFFSQLIPSPQLVVQRDQAAFGARWQLTPILFSQGIHRSQN
                     PWRTFVVEPIVRHSGSLEWFVSPEYLALGDDMPRHFGVRTGLRSYWPLIERGDYLSLS
                     FGTSALRFQQITSVAYETGVHVLFGLVGFMTSCSPTPRAQRCIMTLQVRVF"
     gene            complement(8046..10964)
                     /locus_tag="PK710_02980"
     CDS             complement(8046..10964)
                     /locus_tag="PK710_02980"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT08703.1"
                     /translation="MNDTALRELLTAVREQCSTVTWSEGVELARSHAVVWESEDDGEV
                     CLRVYTGDELRPPKVTLWLDDGDWFCDCGETACAHAAAAVITLRQAQKTGKSLQETVE
                     PAGKIGYRFRRSPQGLLFDRVVVFADREQPLGSSLSAVALGRASGPRFVVQEADMRVE
                     RVLNARLSGWLSRQELASLLEALRDGADVRLDNQPVRLAPAESPVHVLLEDCAAGFRL
                     TAIQDPGISEIFSNGAVLCGQTLRPLGEPGLTVRELEELKKGMIVSFAEAPRLLTDIL
                     PDLESRVTVHRKSQQLPSVDAHALPRIAIETAQDGSALIVFATIVYGDPPIARVDGDH
                     LTHLQGPVPVRRVDMERRLIRTLESDYGLLPGVRSRLLAERAIDMAERLRRPNRELVV
                     HGDGHASFFRAGELMARFSLRGDEFEIDFRTERAGVADPSHVLRAWNSGESMVPLNEG
                     GWASVPSDFLERYGTLLSDLLAARAAAGSKKTSIGYLFDLAKLCEALQVEAPPSYHRL
                     RPLIDHFDGLPQAALPDDLRATLRPYQQVGVNWLSFLRSVGLGALLADDMGLGKTLQA
                     LCVLQGRALVVAPTSVIHNWVAEILRFRPALTYHLYHGPKRTLDTDVHVTITSYAVLR
                     LDSEKLSKIQWDTIVLDEAQTIKNPDSQVARAAYGLQADFRLAMTGTPVENRLEELWS
                     QFHTIQRGLLGSREDFQDRVVRPIQHGNASALVRLRERIRPFFLRRRKAEVASDLPSR
                     TDVVLRCELGEQERALYESIHAATVPSVVEKLRAGGNVMAALEALLRLRQAACHPALL
                     PGQHAAQSAKLDLLLDRLCHARDDGHKALVFSQWTSLLDLIEPLLERESLPFLRLDGS
                     TRDRQSVVQAFQCEEGPPVLLISLKAGGTGLNLTAADHVFLLDPWWNPAVEDQAADRT
                     HRIGQLRPVMVYKLIAKDTVEERIIELQEHKRDLATSALEGGQMAASLTRDDLLSLLT
                     "
     gene            11152..12147
                     /locus_tag="PK710_02985"
     CDS             11152..12147
                     /locus_tag="PK710_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08704.1"
                     /translation="MTQIISSENQRVLLELHALLRDIDPSRWRDGIEAAFRDRLSKIQ
                     AGVAQMRAVARTDGKMDAVRERLDELARAVRDFSPSQSIPCKHTLQEEWLTFRKRVAP
                     YYEACAHALQRKAVRVPSLRPTNYARSLFHVFAGLGSIGLLEFVLPLWSLPWVTGVVA
                     LTCWVLETTRRIWPTWNQTLFKFVFFKVIAHPREVHHVNSATWYATSLFVLSLLQSHL
                     VGMVALAIMAFADPAAAMIGRRWGRTTLLHGRTLEGSLTFVVVGTAAALLGMHILHPE
                     VCSWPMTLAVGACAAVCGSIAELFSRGLDDNLTVPVSAAAGTVLAAWLTGMPMWG"
     gene            complement(12157..12840)
                     /locus_tag="PK710_02990"
     CDS             complement(12157..12840)
                     /locus_tag="PK710_02990"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOT08705.1"
                     /translation="MRIITQEQLDNDTSLYDDAVEIIRNDGLVCFPGHRQYSIAASLM
                     STDAVVALVQSKRRSGKAPSLILIPNESQLSSVAQDVAEVASALIQAFWPGPLTLVVH
                     PNASLPSKVLKLIGEKKPARIGVRVPGHGFQQELVMRFGGPLLISSANLSQKVGAGSA
                     SNVRKNFNHTVDLMFDVGDIPEKAPSTIVDLTREPPTLIREGKIPASQVMDVLQSATH
                     GGETKSEDD"
     gene            complement(12959..13798)
                     /locus_tag="PK710_02995"
     CDS             complement(12959..13798)
                     /locus_tag="PK710_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08706.1"
                     /translation="MHRITVYRWMAAPCTLLAAATAHAQQDVADESFASAHQGQIQLG
                     TGRASLGFGLFTTRTLTGKQEVGGRFTVEIPFGRLWARSASLPHVSQEQSPSPKETTT
                     GHRNPPRMAVRPALARACVRAAWQAHGIGDLEAIDAMRTRAKSSALLPDARFRVVRDW
                     DQSYRLSPTNDDPYRLHESTGGGRTVEGVLTWRLSRLLFVDEELSMERLRVQQVQLRS
                     RLAGEVLRALFDWQRARLALADPSLDEPEHLEAAVREVEAVATLDILTGGWFSSWLGR
                     GDG"
     gene            complement(13861..14706)
                     /locus_tag="PK710_03000"
     CDS             complement(13861..14706)
                     /locus_tag="PK710_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08707.1"
                     /translation="MGTRREELESLSRADLIVQAMELGISAARVLTRDELIDEIVSAV
                     VVDEQERSVARGFLGRARDLITRVMEKGLHLPDAAQRILSPSPPRPEEAPKPIATVAL
                     AAVYAKQGHRSQALCILEEVLAADPDHAAARALQNELGLSTHAEPMAGTCDSLPSDPR
                     TTGKEPEHQSADASSMPDETGIPHRDELSISLGVGQLILCWKVRPITLARWRGKMPSG
                     RLSLRVVHTMVRKGAPKVVVEDHFVDRLVGSMTVSVPDSALSSHAAVGWRDGDLFMVV
                     STTQS"
     gene            complement(14733..15728)
                     /gene="ribF"
                     /locus_tag="PK710_03005"
     CDS             complement(14733..15728)
                     /gene="ribF"
                     /locus_tag="PK710_03005"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HOT08708.1"
                     /translation="MNYSHPLELGTMGARPEDFSGQPTLVAIGNFDGVHLGHQAVLVD
                     TAAEAKSNGWIPVALTFEPHPTVAVGRPPPACLTPVQRKIELIQRIDPSLRVVVQTFD
                     EPFATQSPEAFVRTMLVDRLCARGVRVGENFRFGARRSGDLDSLADFGKQFGFEARAV
                     PLLGDHQGRFSSTRARHAVAIGDLDEVWAILSRPHALSGKVVQGQRRARSLGFPTANL
                     RWVQEALPPHGVYAVVVDRLDEHGAAQRLGAGVCNIGIRPTVDAGFAVEVHLFDFDGD
                     LYGLRLRAHLIHSLRSEKKFNNLDDLKLQIELDVQEARKRLASFLPSVWEGQAWF"
     gene            complement(15874..17373)
                     /locus_tag="PK710_03010"
     CDS             complement(15874..17373)
                     /locus_tag="PK710_03010"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT08709.1"
                     /translation="MADGSAQPSASSRGGPDPLIGRMINDRFKVVALIARGGMGKVYR
                     AEQAPLGRTCALKVLNPKYASEHDPEFHKRFFLEASIVSKLTHPNTVTIFDYGRTDDD
                     VYYMAMEYLEGRTLHRAIREEGAFGEERTAHVARQICRSLREAHSIGVIHRDLKPANI
                     FLVDHADEHDFVKVLDFGLVKNVENPGEDLTQAGLFMGSPKYMAPEQIKGEKVDSRTD
                     IYSLGVIMYEMLTSKVPFDRPNSVHIMMAHVNEPPPPMRSINPDIQASSSTEELILKC
                     LSKSPDQRFPSMEYLLNALKNVGGAAMTATLSGVLSSGDFLAVSSSSGSIATGSSPNQ
                     PMFVTQSGSGESGPLVPSQPTPPENLSARRQSTMILGLIMGGIVAVGLGGAAYYAMRE
                     EPTGPSQLAASASNVVVQPVVASTTANAPAAVNVAALKLDSNPSGAAVMTKEGKMLCS
                     STPCEVKFEGEDAKEDKEHVFVFSKGGFKDTVETVKVSDKTFTAKLVSP"
     gene            17629..18696
                     /gene="holA"
                     /locus_tag="PK710_03015"
     CDS             17629..18696
                     /gene="holA"
                     /locus_tag="PK710_03015"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOT08710.1"
                     /translation="MIPNQAIRDIQRGQLLPVYLVLGEERVLVHRVVAAIRTAALQGG
                     IADFNQEALTAGECDVDRVLSAVRTVPMMAPRRFVLVRSVERWDSSTPKAKGKEKNKE
                     SVVQPLDKLAVYAASPVASACLVLVASKLDGRRKLVALAKKSQFLVECEPLERKALPP
                     FIQQEAKDRGHTIDRDVADLLAEIAGPELSTLLDAIERLSLFVGEGGIITEDTVAACV
                     TRIRPSSVWELVGAVGRRDAATALSVLMDVYEPQDRGLRLVGVLAWSTRQLLRFAHAI
                     RSGASPDQAAVTAGAPPYKARELAKQVKNLDVAEIERWLRVLAETDLALKSSRRPPRS
                     IVESAILTMTGGLSRLEIRPQ"
     gene            18772..19611
                     /locus_tag="PK710_03020"
     CDS             18772..19611
                     /locus_tag="PK710_03020"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT08711.1"
                     /translation="MSDRLMSTGDVAEVLGVSEATVKRWADAGTLSCIRTPGGHRKFR
                     LRDIAMHLASRRSSADHSEAATEGDQDIEELVSALLVGDAERVMGVIAAVRLQRVSIA
                     TIVDTMLRPAFERVKQACGSGRCEAFHLHIAINSLIEVAARQRPTLRVPGSSRGRVIS
                     APLPGEPYDVLARYASLVAVESGFDAHVLGAGLPPASVSRAAEDLGASWILLVGDAHN
                     HLNFSDYAEKILSGTSRSHARIVFISATATPITGLPEDVLTVANLREAESLLAPMALR
                     MAR"
BASE COUNT         4345 a         5562 c         5746 g         4083 t
ORIGIN      
        1 tattggacat gccgatgttg ttggaagagg cgatgagtac gaccaatatt tccaagcgag
       61 tcgcgaggtt acatgcctga cggtcttgct ccccgtgcgg cgtcgctatg gcgtatccga
      121 ggtcgagaca cggacactcg tgtcgagtac ctggggtttt gtttgggcga ggataggtat
      181 gcgcttgcgg tcgatcggac gggcgagatc cttcgaattc cgcccatgac gagggttcct
      241 cggtcttccg atgtggtcat gggtattgtt ggtgtacggg gtcgtgtgct cacggttttg
      301 gatgtgagag ccagacttgg gttaccgaca ccggcgccga cacgccatgc gcggattgtc
      361 gttgtaccat ggtcggagaa tgagtcggtg gggctttatg tcgaccgtgt tgtgcaggtg
      421 tatcgaatga cagcggcaga aatcgaatcc gcggcagcgg gattgggaag aaatgcagga
      481 gaccatgtga tgggcattgc ccgcgtggat ggccacttga tcgtagtcct tcggctcgaa
      541 ccctttcttg cactcatggg cacatgaagc accgaacaca tcacatccgg gcgggtgggc
      601 aacgaagttt ggtgggattt atggtcggcg acgtgaatta cgccatcgat gtgcagcatg
      661 tcgtgcaagt ggtaaatccg atggggatca cgccgctacc ggacacgccg ccttcgatcg
      721 tgggggtgac ggagcatcga ggtcttgtgg cacccattgt cgatcttcgg ctgatgttcg
      781 gcatgcatcc ggaggcaaca cgcaaaacga agtggatttt ggtcgaggtg gggaaccggg
      841 tcacaggcgt tgtcgtggat gcggtcacgg gtgtgtttac ggtatcttcg gaacggatac
      901 agccagcccc ggagcttggt ccaggcgatg tgcgacgacc gttttccggc atgacatcga
      961 ccgaggggcg tttggtattc gtgctcgatc ttgcgaagct ccacgagggt ttcgcgaata
     1021 tcgtcgacat gcaaactagc acgaggtccg aaccgatcag cgtaaagagg gaaggaacgt
     1081 gatcagccac ggttccggac gatctttgcc cacggagttg cccgaacttc gtcccgagga
     1141 atatcgtctg ctgcgcgatc tactcggcga aaaaatcggt catgcgttca acgaagacca
     1201 tgcgcgagag ttcgagcgtc gattgcgacc gcgggtaagc gcctgtggtt tatcgacatt
     1261 tgcggaatat catcggcatc ttcgtgggag ccccaaacgg gaagaagagt tgcagacggt
     1321 ttatgacatt ctcacgaaca acgagacgta tttttttcgc gaggattatc aacttcgctc
     1381 ttttcgcgag gaagtattgc cgaggctcga gcgtgatgcg cggcatcgca aacagctcac
     1441 gtgttggagc atgggatgct cgacggggga ggaagcgtat tcgatcgcca tggtgctatt
     1501 ggaatccggg cgtttcgatg gctggagtat gcgtgtcttg ggcacggatt tgtcgcgcaa
     1561 gcggatcacg ttcgcgcgtc gaggaatcta cggagagagt tcgtttcgat cgacatcgaa
     1621 ggagcgaagg ggagcgtttt tcgtttcgac acgagcgggg acgcgggtat gcgccgcggt
     1681 tcgctcctta tgtcagttcg gccaactcaa cgtcatggag atgaaaaacc gttgcacgat
     1741 tgaccaggtg gatgcggtgt tttgccgcaa tgtattgatc tatttcacac cccaggcgcg
     1801 tgcgcatgtc atggcatcga tcttcgaacg attgcgtccc ggggggttct tgatgctggg
     1861 tcattccgag tccttgctgc acgaccccgg gccatttcaa cccgtccact tgaaacggga
     1921 cgtggtgtat cgtcgcccca cggaacggga gcttcgttcg aagcgggagg ggatatgatg
     1981 gacgcacacg atgatcgcgt gcgcgtgctc atcgtggatg attccacgtt gaatcgccgt
     2041 gggattcgtc gcgttctcga aagcagcgga tgcgtgcagg tggttgggga ggcatcggac
     2101 ggagatgaag cgcttcgcat ggtcacgaac cgcgaaccgg aagccatcac gttggatctg
     2161 gagatgccga ggttggatgg cttttctttt ttacgcattt tgatggcgcg caagccattg
     2221 ccagtcatcg tggtatctag ccacagccag aaggaaaacg tgtttcgggc gctggagctt
     2281 ggcgcgatag atttcgtgga aaagcccgag catggttttt tcgatgaaca agcggcacaa
     2341 caactcgtag acaaagtgct tctcgcgcgc acactccgac ccgtccaggc ccacacgccg
     2401 cccatattcg cttcggagcc agacaccgca cgcggccttg gaaccgtagc gacaacgccg
     2461 ttgccaccac ggtttgtcgt tgccattgcc gcgtctacgg gaggccccac cgccttgttg
     2521 gacgtgttct cgcggctgcc atcacgtatc tcggccgcga ttttggtggc gcaacacatg
     2581 cctcctcgtt tcacgggggc gtttgcggca cgactcgacc gcaagagcgc attgacggta
     2641 cgggaggcta gcgacggcga agcgctgctg gcacgacacg gttatgtttg cccgggcggc
     2701 aagtgcatgg aagtggaggc aaaccaaggg gagatgcggt tacgggtaca gccaccgaat
     2761 cgaaacgatg gctacgttcc gagcgcagat cggctgttca agagtgtggc acagatcgcg
     2821 aaatcgcgtg cgattgccgt agttttgaca ggaatgggga ccgatggggt gcagggggcc
     2881 aaagcgattc gcgatggtag agggctggtg atcgtggaaa gcgcggagac ggccgtggtc
     2941 gatgggatgc caagcgcggt gatccgggcc ggggtggcaa gtcgggtact tccgctgcct
     3001 gccattggag agctattggc aaccttgacg ggatgatgcc gggggggttg gacgcggctc
     3061 gtgggtggcg caagctgtcc gtgttgtcgt gcttatcgcg caacatattg atttaacata
     3121 ttaaattttt gcttctcctc cctgcgaaca ggattttcca tcaagatatg tctggctaat
     3181 tcaatggcat catcacacca acgccaacac ctacgccgac tcgaggtcga cgttgccact
     3241 cacccaacgc gatgacgtga gagacgagca tgcaggttct gctcatgggt ccctggcaac
     3301 cggaccattt tccatcgtga tactcattgc catcctggtc gtagcgaatc agttggcctg
     3361 ccgggtcgat ggccatccaa gcgaaagcgg aaccgggaaa agaagctgag acaggcccga
     3421 cctctcccat gccgatgttg tcttcgccga ccagatgacc gttggggagc aaaagagcgt
     3481 acggatctcc gtttttgtaa tacacgcgtc ccacttgatc gatcagcagc cgttctttgc
     3541 cattttcggt gatggtaccg tccggaagga tctcgatgag gtcgctatcg cgctcccaat
     3601 aggcttgtcc cagggcaatg ggcatgcctc cttgcgccca atgggccggc ggtgtggcag
     3661 aacaagccat gatcgatagt gccaaccaga gaagggatga agcgatggca cgagacgaaa
     3721 cgagaaagcc attcatcggg ggatagcata gcaacaactg gtttgggcgg cacgggtggc
     3781 ttacgacggg ctcttgcgct atggcccgcg ctgatcgagg agacagtcat gaatgttcgt
     3841 cttgtgaagg agtatcgatt cgaagccgcg catcgtttga cccgtgtgag cgatgagcat
     3901 ccgtgtggcc ggctacatgg tcatagctat cgtgtcgagc tttccctaca agggccggtg
     3961 gatccgcggg tcggatggtt gattgatttt ggacggatcg atgaggcttg gaagcctgtc
     4021 aagtcgcgtc ttgatcacgc gatactcaac gagctgccgg gtttggagaa tcccaccagt
     4081 gaaaacctcg cggcctggat ttgggatgcg ttgatagatg cccttcccat gctatgtcgg
     4141 gtcacgttgt gggaaaccgc cgatgcacga tgcgaattcg aaggtcatgg ggcccggtag
     4201 ggatcagtgc gacagaggct gaacgaacgg tccgtcttca aagacactcg cgatgttgca
     4261 taccctcggg ctgttgcatc cttgggggcg caacgcaaag gctcgatcgg gcatcgcatg
     4321 aagcacggat gaagtaggga aatcctttct catcacccca tctccaacca cggatgattc
     4381 gatctgacgg aggatatcat ggccgttgaa ttcaaaaact ttccgctcga aggcaagctc
     4441 aagggtatct cggatcgcca gcttcaagag catcgagaca cgctctacaa ggggtatgtc
     4501 aatcaataca accgcatctc cgagctgatt accaaaggcg atagcagtac ggcaaacccc
     4561 cacttttcgg aatggggcga actcaagcga cgagaaacct gggctcataa tggtgcgcta
     4621 ttgcatcaga tgtatttcga gaacctgacg gaagccaact cgaccaagcc gggaacgaaa
     4681 acgaccaagt tgatcgagcg tgattatggt tccctcgagg cattcaaggc ggatttgctc
     4741 aacgccggca aggtggctcc cgtgggctgg gctgtatggg cctactccgt gctcgacaag
     4801 aagacgcatg tatttttgat tgaacaacac atgaaccaca cccccatcgg cgtgattccc
     4861 cttctgattc tcgacgtatt cgagcatgcc tatatgattg attacggcac cgcgcgtccg
     4921 ccttatctcg aggctttctg ggagaatctg aactggaata cggtcgaaga gcgtgtggcg
     4981 atggtgccag ggctctgatc cacgactact cggaagcaat ctgcgacagg atgcgagcaa
     5041 tggatacgaa ttgctctccc tcctcgcgga gcaccgcacc gtagaaatcg caagatagac
     5101 aattacccat cttgctggcg aaggtcccgg caatctggcc atcgcacatc gttcccgcca
     5161 cagcccaaca cattcttccc gcgttttttc caccatgaat gcagtccgct tcgaccgtga
     5221 tggcggcggg acagacaccg agctgcgcga cgtgaatacc tcccggctca cgaccgcact
     5281 tgatgaattc ccagcagttg agtttttggt ttctgcgcgc aggcattgct gaaccaccca
     5341 ttgccttgta ggtgccgtaa ggatcgctgg agcgtgcgtg aacgatccct tcgatcaaaa
     5401 cggtcgagcc gccagctcga tcattggaaa ttgatgacga tccatccgcc ctggctgctg
     5461 aaaccacagg cgactttgct gaggtacttg gcgctcatgt tgacgtaatg gccgcagact
     5521 tgtccggtgc tgcttccgta aaagtcgcag cctgggcaat tgggcgaata ctggtccgca
     5581 cacgcatcac acccctggca accgctttgg tctttttccg cccacatgga ttcgaggcac
     5641 gcttccacac tggaagcacc ccatccaggg cattcgtttt gtcccatttc accgcattgc
     5701 ccaaaagcgc catgggcctg acccgaagcc gcatctttcg aggcttcgtc atcggaacaa
     5761 gattccgctg ctgtccaacg cgcgtagggt ggcaagcctt tggtggcgcg aagcgcattc
     5821 actttgttga aacatgcttc tcgttccatg gcaaaggggt ccgacggatc tccgcccgaa
     5881 ccaccttgcg cgctgccgcc cgaaccgcct tgcgcgctgc cgcccgaacc gccttgcgtg
     5941 ctgccgcccg aaccaccttg cgcgctgccg cccgaaccac cttgcgcgct gccgcccgaa
     6001 ccgccttgcg cgccgccgcc cgaaccgcct tgttcgctgc cgcccgaacc gccttgttcg
     6061 ctgccacccg agccgccttg cgctccagcg tctttcccac cggatccccc atgagaggag
     6121 gagggagaac ccccgttttc atcgtcatcg ttcgagcagc caaccatcag cccgagcagc
     6181 gccaaagttc ccacgagcaa tcgttttcgc atgacataac ggtaccacga ttggagacgg
     6241 aacaaggaca aggtagacgc gccgatggcg attggatcga ttcacccatg atgggagtgc
     6301 gaatccaggc ggatggtgga tgtgaaaaaa ggtgcatcat ggcggtgttc cacgacggaa
     6361 ccgagcctga tgaaagaggc gctacaatcc caaataccct ataatttcaa aaggatacga
     6421 ttaccgggag atcaatactt tctgttcgcc gatcgccagc cgatgcacac gattttccgc
     6481 gatacgattt tgtttcacgg cttgttcgag cacgcgagtt ggctctcccg gttcgtcgag
     6541 cgaattgatg aacgtatcga agtgaatcgg caccatccat gttgcgccaa gcttctcgaa
     6601 agcagtgagg gcttcccaag ggtccgtgtg gacacgtttc atgaagccgc gaggatggat
     6661 gggcgcaatg ggcaagagag caagatcgat ggtgggaaaa cgccgggcgg tctcgcggta
     6721 ggcatcttct gccaaggcgg aatcgccacc gaagtacacg gtaattccgt ggtattcgat
     6781 cacccacgcg gcgtatccga tgccctctct ccacgcatgg tccaaaccgt aacgccatcc
     6841 attgtggcgc acggcgacgg cggtcacctt caaatctcct ggcaatacac gagtttgcca
     6901 ccagggaacc tcgtgggcag gaaaagaaaa gtcggtgaga tatacgagtc ccccttcggg
     6961 catcccgagc caacgcacct tgtcttcgat catttcaagg gagccaagcg aaagatgatc
     7021 gaaatgcatg tgcgagataa gcacggcatc gaggggcggt agatggcgaa cctcgatgcc
     7081 gggttcgacc aggcgcggag aaatttgccc tacggttcgc gtccatacag gatccgtcaa
     7141 taggatctta tcgtctatct gaatcagaac ggtggcatga cccacccaca gcaccgcaag
     7201 acgcgcttcg ggtcgaaccg ggttttcgat cttgtgggcg acggtacgta gctgcccaaa
     7261 ggccgcgaga tttcgcgaaa gaaattgtgt tgcgatgcag cccgtcgaaa gcaaagccag
     7321 ccatactacc cacacgagtc gtcgcatcat caaaacactc gcacctgcaa agtcatgatg
     7381 catcgttgcg ctcgtggtgt gggggagcaa ctggtcatga agcccaccag accaaaaagg
     7441 acatgaaccc ctgtctcata agccacagag gtaatctgct gaaaacgaag ggcagaagta
     7501 ccaaaggaca aggaaagata gtcaccgcgt tcgatgaggg gccaatagga gcgaagcccc
     7561 gtacgtacgc caaagtgacg cggcatatca tctcccaacg cgagatactc tggggagacg
     7621 aaccattcga gggatccgct gtgacgcacg atgggttcga cgacgaaggt tcgccatggg
     7681 ttttggctac gatggatgcc ctgggaaaat agaatggggg tgagttgcca gcgagcgccg
     7741 aaagcggctt ggtctcgttg aacaacgagt tgagggctcg ggatgagttg tgaaaaaaac
     7801 caaggccatg agggtcgagg atgagcaccg ccatcggatt gcgcgagggc gtcgtggcag
     7861 aagagaaaaa acaccagtcc caggacaacc gagataggaa ccttgcctcg gcagcaacgc
     7921 ggcgtacgac aaacgccatc atcggaatcg aaagagccat ccatcatgat aacgcggtaa
     7981 ggcatggtcg agatcccgtg atgttcggag ggcgcggagc cacgatcgaa caacaatgaa
     8041 aagcgtcacg tgagcaagga aagcaaatcg tcgcgggtga gagaggcggc catctgccca
     8101 ccttcgagcg cggaagtagc gaggtcgcgt ttgtgctctt gcaattcgat gatgcgctct
     8161 tcgaccgtat ccttggcgat gagcttatag accatgacag gtcggagttg gccgatgcgg
     8221 tgcgttcggt cggccgcctg gtcttcgaca gcgggattcc accatggatc caagagaaaa
     8281 acgtggtccg cggccgtgag gttcaatcca gtgccaccgg ctttcagcga gatcaacaga
     8341 acgggtggac cttcttcgca ctgaaacgcc tgcacgacgg actgccggtc ccgcgtggaa
     8401 ccatcgaggc gcaaaaaggg tagcgattcg cgttcgagca gcggttcgat caagtcgagc
     8461 agcgaagtcc attgagaaaa gaccaacgct ttatgcccat cgtcgcgcgc gtggcagagc
     8521 ctatcgagca aaagatcgag tttggcggat tgcgcggcat gctgcccggg caacaaggcg
     8581 ggatggcaag cagcttgccg aagtcgtaac agcgcttcca aagccgccat gacatttcca
     8641 ccggcgcgca gtttttccac gaccgaaggc acggtggcgg catgaatact ttcatacaac
     8701 gccctttcct gctcccccaa ttcacaacgc agcacgacat ccgtgcggga aggcaggtca
     8761 gacgctacct cggccttgcg ccgtcgcaag aaaaacggcc gtattcgctc tcgtaaccgc
     8821 accaacgcgc ttgcgtttcc atgctgaatc ggtcgaacca cacggtcttg aaaatcctcg
     8881 cgactcccca aaagcccgcg ttggatggta tgaaattgac tccataactc ttcgagacgg
     8941 ttttccaccg gcgttccagt catggccaat cgaaaatctg cttgcaaacc ataagccgct
     9001 cgagccacct ggctatcggg gtttttgatc gtctgggcct cgtccaacac aatcgtatcc
     9061 cattgaattt tcgatagttt ttcagaatcc agccggagga cggcatagga agtgatcgtg
     9121 acatgtacgt cggtatcgag ggtacgtttg gggccgtggt aaagatggta ggtcaacgca
     9181 gggcggaatc gaaggatttc ggccacccaa ttgtggatga cgctagtagg agccaccacg
     9241 agcgcacgcc cctgcagaac gcacaaagct tgcagggttt tgccgagtcc catgtcatcg
     9301 gcgagcaacg caccaagccc tacggaacgc aaaaacgata accaattgac gcccacctgt
     9361 tgataggggc gaagagtggc ccgcaggtca tcgggcaaag cagcttgggg gaggccgtcg
     9421 aagtggtcga tgagaggccg gagtcgatgg tagctgggag gtgcctcgac ctggagggct
     9481 tcgcatagtt ttgcgaggtc gaatagataa ccgatcgaag tttttttcga gcccgcggcg
     9541 gcgcgcgcgg ccaacaaatc cgaaagcaag gttccatatc gttcgagaaa atccgaggga
     9601 accgaagccc atcctccttc gttcaagggc accatcgatt ccccactgtt ccaagcccgc
     9661 agcacgtggg aggggtctgc cacgccggcc cgctcggtgc gaaagtcgat ttcgaattca
     9721 tcgccacgca aagaaaaacg agccatcaac tcgccggccc ggaaaaagga agcgtgccca
     9781 tcgccatgga ccaccagttc gcgattcgga cgtcgcaagc gctcggccat atcgatcgca
     9841 cgctcggcca acaagcggga tcgaactccc ggtagcaacc catagtcgga ttcgagggtg
     9901 cggatgagac ggcgttccat gtcgacacga cgaaccggca cgggtccttg caggtgggtc
     9961 aaatggtcgc catcgacgcg ggcgatggga ggatcaccgt atacgatcgt ggcaaaaacg
    10021 atgagcgcac tgccgtcttg cgcggtttcg atggcgatgc ggggcaaggc atgggcatcc
    10081 accgaaggaa gttgctggct ttttcggtgt acggtgacgc gagattccaa gtcgggaaga
    10141 atatcggtga gcaaacgggg agcttcggca aaggaaacga tcattccttt ttttagttcc
    10201 tcgagttctc gaacagtgag ccccggttcg ccgagcggac gcagggtctg gccgcagagc
    10261 accgcgccat tggaaaatat ttcagaaatt ccggggtctt gtattgccgt aagtcgaaat
    10321 cccgcggcgc aatcttccag cagcacatgt acgggagact cggcgggggc gagacgcacg
    10381 ggctgattat cgaggcgcac gtccgcgcca tcgcgcaaag cctcgagcaa ggatgcgagt
    10441 tcttgtcgag acaaccatcc cgagagccga gcgttgagaa cgcgttccac ccgcatatcc
    10501 gcttcctgca ccacgaagcg ggggccgctg gcccggccca aggctaccgc tgacaggctg
    10561 gaacccaaag gctgctcacg gtcggcaaag acgaccacgc ggtcgaacag caacccttgt
    10621 ggcgatcgac gaaaccgata gccaattttt cctgctggtt cgacggtttc ttgcagggat
    10681 ttgcctgttt tttgggcttg tcgaagggta atcaccgcgg cggcggcgtg agcacaagcc
    10741 gtttcgccac aatcacaaaa ccaatcccca tcatccaacc acaaggtcac cttgggaggc
    10801 cgaagctcat cgcccgtata aacccgcaag catacttcac catcgtcttc tgattcccaa
    10861 acgaccgcat gggatcgagc aagctcaaca ccttcggacc aggtgaccgt cgagcattgc
    10921 tcacgaaccg cggtcaatag ttcacgcaaa gcggtatcgt tcatggtttt tcgggatagg
    10981 gtcgaaacga aggcagaatt tacagatggg tactgtaaat gaaatgtaaa acccaacgga
    11041 gagccaccaa aggtcttgtt ttatgcttcg cagcaagaat ttgacgcaaa aatcgtgcgt
    11101 cttatcgctt catcggctac accagggcaa ctcttgccct ctgtgtgaag catgacgcag
    11161 atcatctcga gtgagaatca acgagtcctt ctcgaactgc acgctctgtt gcgcgacatc
    11221 gatccttctc gctggcgcga tggcatcgaa gcggcgtttc gggatcgtct gagcaaaatc
    11281 caagcgggag tcgcgcagat gagggcggtc gcacgaacgg atggaaagat ggatgccgtt
    11341 cgggagcgtc tcgatgagct ggcgcgcgcg gtgcgggatt tttctccctc gcagagcatt
    11401 ccctgcaagc atacgttgca ggaggaatgg ttgacatttc gcaagcgggt tgcgccctac
    11461 tatgaggcat gtgcgcacgc gcttcagcga aaagcggtac gagtgccgag tttgcgcccc
    11521 accaactatg cgcgaagttt atttcatgtt ttcgcgggct tagggtcgat cggcttgctc
    11581 gaattcgtct tgccgctgtg gtccttgcca tgggttaccg gtgtggtggc tctcacctgt
    11641 tgggtcttgg aaaccacacg ccgtatttgg cccacgtgga accaaacctt gttcaagttc
    11701 gtgtttttca aggtgatcgc ccacccccgg gaagtgcatc acgtcaattc cgccacctgg
    11761 tacgcaacat ccttgttcgt gctatcgctg ctgcaatcgc atctcgtggg catggtcgca
    11821 ttagccatca tggccttcgc agatcccgcg gccgccatga tcggacgtcg ttggggacga
    11881 acgactctgc tgcatggccg caccctggaa ggatcgttga cgttcgtggt cgtgggaact
    11941 gctgcggctt tgctgggcat gcatatcctc cacccggaag tatgctcatg gccgatgact
    12001 ctcgctgtcg gcgcctgtgc cgcggtatgc ggcagcattg ccgaactgtt cagccgtggt
    12061 ctcgatgaca acctcacggt ccccgtctcc gctgccgccg gcacggtgct cgctgcatgg
    12121 ctcacgggca tgcccatgtg gggttgaacc caccgttcaa tcgtcctccg acttggtttc
    12181 ccctccatgc gttgcgctct gcagcacatc catcacttgc gacgctggga tcttgccttc
    12241 tcggatcagc gttggcggtt cgcgggtcaa gtccacgatc gtagatggcg ccttttcggg
    12301 aatgtctccc acgtcgaaca tcagatccac cgtatggttg aaattttttc gcacgttcga
    12361 agcagacccc gcccccactt tctgagacaa gttcgcgctg ctgatgagaa gtggaccgcc
    12421 aaatcgcatg accaattctt gctgaaatcc gtggcccgga acgcggaccc caattcgtgc
    12481 cggctttttt tccccaataa gtttcaatac tttagaagga agcgaggcat tgggatgcac
    12541 caccaaggtg agaggaccag gccaaaacgc ttggatcaat gccgaagcca cttccgctac
    12601 gtcctgtgcc accgaagata attgagattc gttcgggatc aggatcaacg acggtgcttt
    12661 gcccgatcgc cgtttcgatt gcaccaaggc cacgaccgca tccgtggaca tcagcgaagc
    12721 cgcgatgctg tactgtcggt ggcccggaaa acaaaccaaa ccatcgtttc ggataatttc
    12781 caccgcgtcg tcgtacaggg aagtgtcgtt gtcgagctgt tcctgggtga tgattcgcat
    12841 ggggacgagg ccgcatacca tggaacccat cgcggcgtca caagctgttg cgtgccaaaa
    12901 attccgccgc ggtcgcgcaa accgcccagc gcgttttttg ccccgcgggg aacgatttct
    12961 aaccgtcgcc tcgaccaagc caggaggaaa accaccctcc ggtcaagata tccagcgtcg
    13021 ccacggcttc cacctctcgc accgccgcct ctagatgctc cggctcatcc agcgacgggt
    13081 ccgcgagagc gagcctggcc cgctgccaat cgaaaagagc gcgcaacacc tcgccagcaa
    13141 gacgactgcg aagttgcact tgctggaccc gtagccgctc catcgacagc tcctcgtcga
    13201 cgaacagcaa acgacttagc ctccatgtca acacgccctc cactgtccgt ccccctcccg
    13261 tgctctcatg caaccgatag ggatcgtcgt tggtcggtga aagacgatag ctctggtccc
    13321 aatctcggac gacacgaaac cgagcatcgg gaagcaaggc agaggatttg gcgcgtgttc
    13381 gcatggcatc gatcgcttcg agatcaccga tgccatgggc ttgccacgcg gcacgcacgc
    13441 acgcacgagc caaggcggga cgtacagcca tccgcggtgg gttgcggtgg cccgtggtag
    13501 tctctttggg cgaaggactt tgttcttggg aaacgtgcgg caaacttgct gagcgcgccc
    13561 aaagccgtcc gaagggaatc tccacggtaa aacgtccgcc aacctcttgt tttcccgtca
    13621 acgttcgagt tgtaaacagc ccaaatccca gggaagcacg cccggttcca agctgaatct
    13681 gtccttggtg cgcagaagca aaggattcat cggctacatc ttgctgggca tgggcggtcg
    13741 ccgcggctag cagggtacaa ggcgcagcca tccatcgata cacggtgatg cggtgcatga
    13801 cacgatatcg gacgcacgga ggtttggttg cccaaaaatc gtcaaatacg gctactcgaa
    13861 tcaggactgc gttgtggaca cgaccatgaa caagtcgcca tcacgccatc ctaccgcggc
    13921 gtgggaactg agggcggagt cgggtacgga cacggtcatg gagccaacca atcgatccac
    13981 gaaatggtct tcgaccacga ctttcggcgc accttttcgg accatcgtat gtacgacacg
    14041 cagggacaga cgtcccgaag gcattttgcc gcgccatcga gccaaggtga tgggacgcac
    14101 tttccaacac aatatgagct gtcccacacc caatgaaatc gatagttcat cgcgatgcgg
    14161 aataccggtt tcgtcaggca tggaagacgc atcggcggat tggtgctcag gctctttccc
    14221 cgtggttctt ggatcgcttg gcagcgaatc acaggtgcca gccatcggtt cggcgtgggt
    14281 cgaaaggcca agttcgtttt gcaaagcacg cgcggcggca tgatctgggt ccgcggccag
    14341 cacttcttca agaatgcaca aggcctggga tcgatgacct tgcttggcgt acacggccgc
    14401 cagcgcaacc gtcgcaatcg gttttggagc ctcctcgggt cgagggggag acggtgacag
    14461 aatgcgctgt gcagcgtcgg gcaagtgcaa ccctttttcc atcacgcgtg tgatgagatc
    14521 tcgcgctcgc ccaagaaatc ctcttgccac cgaacgttct tgttcgtcga ccacgaccgc
    14581 ggaaacgatt tcatcaatca actcgtcgcg agtcaacaca cgagcagccg agatacccaa
    14641 ctccatcgct tgcacgatga ggtccgctcg cgatagcgat tccaactcct ctcggcgcgt
    14701 acccatggct ctaccgtaac tgaagatggg gtttagaacc aggcttggcc ttcccacaca
    14761 gaaggcaaaa aggaagcgag ccgctttcga gcctcctgca catcgagttc aatttggagc
    14821 ttgagatcgt cgagattgtt gaattttttt tctgaacgaa gcgagtgaat gaggtgcgca
    14881 cggagccgca acccgtataa atctccatca aaatcaaata gatgcacctc aaccgcaaat
    14941 cctgcatcca cggtggggcg aatcccgatg ttgcatactc ccgcgcccaa tcgctgtgct
    15001 gcaccatgtt catcgagacg gtccacgacg acagcataaa cgccatgagg aggaagagct
    15061 tcctgtaccc atcggaggtt ggcggtagga aatccaaggc tgcgagcgcg acgctgtccc
    15121 tgcacgactt tcccggatag ggcgtggggt ctggataaga tggcccagac ctcgtcgaga
    15181 tctccgatgg ccacggcatg acgcgctcgc gtcgaggaaa aacggccctg atgatcccca
    15241 agcaaaggca cggctcgggc ttcgaaaccg aattgctttc cgaaatccgc gagactgtcg
    15301 agatcgcccg aacgtcgagc gccaaagcgg aagttttctc ccacgcgaac gcctcgagca
    15361 cagagccgat cgacaagcat ggtacggaca aatgcctctg gagattgcgt ggcaaacggc
    15421 tcatcgaacg tttgcaccac cacgcgtaag gacgggtcga ttcgttgaat gagttcgatt
    15481 tttcgctgaa cgggtgtcaa acaggctggt gggggccttc ccacggccac cgtcggatgc
    15541 ggttcgaacg tgagggcgac gggaatccag ccgttggatt ttgcctcggc ggccgtatcc
    15601 acaagtacag cctgatggcc aagatgaaca ccgtcgaagt tgccaatggc cacgagagtg
    15661 ggttgaccgg agaagtcctc tggacgagcg cccatggtgc cgagttcaag ggggtgagag
    15721 taattcatga acaagacaat ccctcgttgg ccgtgagcga agcgattgtc aaggcgattg
    15781 gacgcacgag ggtgcgctcc acgacttgtt ggcatcgggg cagtaggcgt taccgcggca
    15841 gaaatccgat aggggccgtc gaagggcgag gcttcatggg gaaacgagct tcgcggtgaa
    15901 tgtcttatcc gacaccttga ccgtttccac ggtgtctttg aatccccctt tggaaaagac
    15961 aaacacgtgt tccttgtctt ctttggcgtc ttccccttcg aacttgacct cacagggagt
    16021 ggaagaacac aacatttttc cctctttggt catgacggcg gcaccggacg ggttggaatc
    16081 gagcttcaga gccgccacgt tgacggcagc gggggcgttt gcggtggttg atgccaccac
    16141 gggttgcacc acgacgttgc tggcgctggc ggcaagctga gaaggacccg taggttcctc
    16201 acgcatggcg tagtaggccg cgcccccaag tcccaccgcg acgataccgc ccatgatgag
    16261 gccgaggatc atggtggact gacggcgagc ggataggttt tcgggcggag tgggctgtga
    16321 gggaacgagg ggcccagact cgccactgcc agactgagtg acgaacatag gctgatttgg
    16381 actggatcct gtggcgatgg agccggacga actggataca gccagaaagt cacccgaaga
    16441 cagaacgccc gatagggtgg cggtcatggc agctccgccc acgtttttca gcgcattgag
    16501 caagtattcc atggagggaa agcgttgatc gggacttttc gaaaggcatt tgaggatgag
    16561 ttcctcggtc gatgacgacg cctggatatc gggattgatg ctgcgcatgg gaggaggcgg
    16621 ttcattgaca tgcgccatca tgatgtgaac ggagttgggc cggtcaaaag gcactttgct
    16681 ggtcagcatt tcgtacatga tgacacccag cgaatagatg tccgtacggc tatcgacctt
    16741 ctcgcccttg atttgttcgg gggccatata cttgggagag cccatgaaaa gacccgcttg
    16801 ggtgaggtct tcccccgggt tttcgacatt tttcacgagt ccgaaatcga gcactttgac
    16861 gaagtcatgc tcatcggcgt ggtccaccaa aaaaatgttg gcaggtttca agtcccgatg
    16921 aatgacacca atcgaatgag cctctcgaag gcttcggcat atttgtcttg cgacatgcgc
    16981 cgttcgctcc tcgccaaacg cgccctcttc gcgaatggcc cggtgtaacg ttcgcccttc
    17041 gagatactcc atcgccatgt aatacacatc gtcgtcggtt cgaccgtaat cgaagatcgt
    17101 caccgtgttg ggatgcgtga gtttggagac aatggaagct tccaaaaaga aacgtttgtg
    17161 gaactcggga tcgtgttcgc tcgcatattt cggattcagt accttcagcg cacaggtacg
    17221 acccagaggg gcttgctccg cgcgatagac tttacccatc cctccgcgag caatcagcgc
    17281 cacgactttg aagcggtcgt tgatcatacg accgatcagc ggatcgggtc cgccacgact
    17341 cgaagctgaa ggttgcgctg aaccatctgc catgggaaat tgctccaaag aggaaatact
    17401 ccgttagata tcccaaggaa tattcccaaa aggagtctgc ccgcgtgcat tgggcgtgtc
    17461 aatctcccag tcaaggctcg aggcaagacc acccgcacgc ccttgtgaaa cagtccacca
    17521 aaccgcgagc acgaatcgct ttcgaacggc cacaacacaa cggccatggg aaaaggcgga
    17581 ttgtacccct tgatctcaac cacctaacga ggctacgccc cccgtagcgt gatcccgaac
    17641 caagccattc gcgacattca acgcggacaa cttctccctg tctatctcgt gttgggagaa
    17701 gagcgagtcc tcgttcatcg tgtcgttgcc gcgatacgta cggccgccct gcaaggtggc
    17761 atcgcggact tcaaccaaga ggctctcacc gcaggcgaat gcgatgtcga tcgcgtgctg
    17821 tcagccgtac gcaccgtgcc catgatggca ccccgacgct tcgtgctcgt tcgatcggtc
    17881 gaacgatggg atagttcgac gccaaaggcc aaaggcaaag aaaaaaacaa agagtccgtc
    17941 gtacaacccc tcgacaaact tgccgtctac gctgcttcgc ccgttgcttc cgcatgcctc
    18001 gtgcttgtcg cgtccaaact cgatggtcga cgaaaactcg tcgccctcgc caaaaagtcc
    18061 cagttcctcg tggaatgtga accgctcgag cgtaaggcgc tgcctccgtt catccaacaa
    18121 gaagccaaag ataggggaca taccatcgac cgagatgtgg ccgatctgct cgcggaaatc
    18181 gccggtccgg aattgtcgac cctgctcgat gccatcgagc gtctttcgtt atttgtcggc
    18241 gaaggtggaa tcatcacgga agataccgta gccgcctgcg tcacccgaat acgaccgtcg
    18301 tccgtatggg aattggtggg cgccgtgggg cgtcgcgatg ccgctaccgc gttgtccgtg
    18361 ctcatggacg tgtacgagcc ccaagataga gggctgcgct tggtaggcgt gcttgcctgg
    18421 tcgacacggc aacttcttcg gtttgcccat gccattcggt ccggagccag ccccgaccaa
    18481 gcggccgtaa ccgctggagc ccctccctac aaagcgcgag aattggccaa gcaggtcaaa
    18541 aatctcgatg tcgcagaaat cgaacgttgg ctgcgcgttc ttgcagaaac cgacctggcc
    18601 ttgaaaagct cccgacgccc cccgcgtagt attgtcgaat ccgccatctt gacgatgacc
    18661 ggtgggttat ccaggttgga aattagaccc caataaacgt gaagccacac gtccggggag
    18721 agaaagaccc atgggtgagg ccaatcccac cgttttttta ggagcattgt catgagtgat
    18781 cgcctcatgt ctaccggaga tgttgctgaa gtccttggcg tcagtgaagc taccgtaaaa
    18841 cggtgggccg atgctggtac gctatcgtgt atccgtacac caggaggaca ccgcaagttt
    18901 cgtcttcgcg atatcgccat gcacctagcc tcacgacgtt cttccgcgga tcattcggaa
    18961 gcggccaccg agggtgatca agatatcgaa gaattggtca gcgccctttt ggtcggtgac
    19021 gcggaacggg tcatgggggt cattgcagcc gttcgtttgc agcgcgtatc gatcgcaacc
    19081 atcgtagata cgatgcttcg gcccgccttt gaacgcgtaa aacaagcgtg tggctcaggc
    19141 cgttgcgaag cgtttcattt gcatattgcc atcaatagtc tgattgaagt cgccgcacgc
    19201 caacgcccca ccttgcgcgt gcctggcagc agccgcggaa gggtcatttc cgctcccctt
    19261 cccggggaac cctacgatgt tctcgcaaga tacgcgtcgc tcgtagccgt ggaaagcggt
    19321 ttcgacgctc atgtgctcgg agccggactg ccccctgcaa gcgtctcacg cgcggccgaa
    19381 gacctgggcg catcctggat ccttctggtc ggcgatgctc ataaccacct gaatttctct
    19441 gactacgcgg aaaagatcct gtcgggaacg tcgcgatcgc acgcccgcat cgtgttcatt
    19501 agcgcgacag ccacgcccat caccggcctt cccgaagacg tgctaaccgt tgccaacttg
    19561 cgggaagccg aaagcctact cgcgcccatg gcattacgaa tggcccgctg acccactgtc
    19621 ctaccccctt tcattctccc tcgcttcccc ggcccgtatg aggcaccttc ttgcctacca
    19681 tgagtcaaac catgcccaag gcgcctgcac cttacaccac cgattccgcc ccccac
//
LOCUS       DAOSOR010000031        19359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_2828_length_19359_cov_5.200839, whole genome shotgun sequence.
ACCESSION   DAOSOR010000031 DAOSOR010000000
VERSION     DAOSOR010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 19359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19359
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2828_length_19359_cov_5.200839"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PK710_03025"
     CDS             <1..349
                     /locus_tag="PK710_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08712.1"
                     /translation="GESGTVHVASHAGIVYSSESTTLDREVALDTSPDVSEGDGGVIP
                     SYGLPSPPLLADRSGQVAFVRADGRVGVISTNGTVRFASRSSLATPVDLAAAGPNRFV
                     VVCRTGDIFLYGP"
     gene            complement(337..1146)
                     /locus_tag="PK710_03030"
     CDS             complement(337..1146)
                     /locus_tag="PK710_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08713.1"
                     /translation="MKRYLPLSLIAFALVACETNEQPNPAPTVDIPVDAAAETTIIDV
                     FSEDVFLPPTEAGPFRRSVLLRSPFGNLAHTDNLLLDGDMEMSVAQGQTPWRVLGLGG
                     QVSVPVETGGRCRSGLRCIALSAKANAAFGVGVAARNLPLEFWLWAKTPGACSALTVY
                     MFSAMTMNITQFHQVQADNPQPDASGWCRFSALRSASDESIGIYVEAKVTSVQQILID
                     DAVLRPADASSPTTKGLIAVRPQVHQRIVNRLAPWMRQRWIGPPPPWPQGP"
     gene            complement(1143..3188)
                     /locus_tag="PK710_03035"
     CDS             complement(1143..3188)
                     /locus_tag="PK710_03035"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOT08714.1"
                     /translation="MNPRSLWAWFAPLMGLFLPTNAFAHFDPQGNYTFDPRAVHTESF
                     ESLPSWNPAQPDPNAIKNLFTLNDPGALEGNRVGAVSPEWWAEGVRLPVTLPPLLASY
                     RIRLWIRSGWLAATFVVDDAADPLQRSVADLSATGRCTSDGWVEMASAPVTIDGLTTS
                     AAFLYLMGDALIDGFEIVEEGVVKPSAPCLGARDLACGKDEVCIAGLCRAGDTLVPHW
                     PPPAERSRVVDFLFTRLQYFFGGHQTRRDHLPDALAQVEAMRTATSAWEFWHGFATAV
                     RKLHDWHTSASGAIQFLQSPYVLNACFIEGKADLSQHAWPSDSSSFDWLVSHVGPDRN
                     VGLKPGDRLVAIDGMHPLQWARSLIDVSWDWWQADDDRVNAEFAEVIRGLIPKYAQNF
                     SIVRCHAETGTCDDTPDLIEVSSLPRRDEIDEPLVACDNRPSYHLVQGPDPTTHNVGW
                     NVFVGLLEDSQPGENLYGITWDSLWGPTTTPIFLKANETFKANARGVILDHRAGNGGT
                     IDAPEAITQLVREPLDIAIFIHDRPWADYEGASTVEEGLAIFERYKKNTHYPDLTYSV
                     GSAKAKLDLPVALLQHRDGSASDYLPFGMKGSPRVRIFGPQPTAGAFSSFFQMALGGN
                     MRLSLASGDTIAYDGQALIGHGVEPDEIVVPLQSDLIHGKDTLYLRALAWLRSELDP"
     gene            3528..4178
                     /locus_tag="PK710_03040"
     CDS             3528..4178
                     /locus_tag="PK710_03040"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT08715.1"
                     /translation="MLRLRTLGIAMALVLGASGVMLTTGCGEAVLQVGVNPPPGPPPA
                     PPPPPEPEPVAAPEPPPEPPPLVEVKGDEIKINDKINFAKNSAEIESSSNSLIENIAN
                     VMKKHADIDFVEVAGHASREGDEFYNKNLTQRRAKAVMDALVALGVEKNRMRAVGYGF
                     YCELQPDNHEANRRVEFKILRRAGKDTSVKGGGCEAADKKGLTAKPIPADAPKTEK"
     gene            complement(4260..5309)
                     /locus_tag="PK710_03045"
     CDS             complement(4260..5309)
                     /locus_tag="PK710_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08716.1"
                     /translation="MLHARSYPWVLCACVAIGSATMTTSGSVGAEPPTKQEARTLFQE
                     ALAHVAAGDYTTALGKLERVATFKRTPQVGFYIGYCHEKLGKLVMALGEYRIALADAQ
                     ASAASDVIAEAQEAIGRVEPRIPRLIIHRGDGAATATVLVDRKPLGESAIGQPMPFDP
                     GTRVVVAQAAGYLPFRQEVRLAEGEKATIEIKMIKRAPGAAAPIPPQNTPERTDSSSS
                     SSVEIDSREGSNTLAWIATGVGVVGLGASGYFMVQRSKAISDLDDACGSGRAACPSSM
                     KKTYDTGKRDTLFANVALGVGAAGIVTGALLFIFSGGSQPDNTLTQPDQPGLSFSPQP
                     PPQGGWAGVRMDGRF"
     gene            5411..7720
                     /locus_tag="PK710_03050"
     CDS             5411..7720
                     /locus_tag="PK710_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08717.1"
                     /translation="MKLAPVLPLKKKSRTRQRVERVLLFISRKKKAKSVNQEPPKASA
                     RPPRLSPSVRPTRASTAPPSLLDASAALMGDTDVPFAPSSATLPKKTRKKPKKNPFLS
                     TPVPETFRPPAPTQQAPSVLSAPLAAAPKTKENQLPAAPPSFPSVASRIEAEAQTISE
                     RKPPARPSPPTPSYAPVSRDVSVQHTPRSTFRESYSRQDSPVLPRYGSTTVPVRAVAI
                     AHEKSNAVGTIELSCVAHGLRIQFVRISSYTEGFVPYPATSSERITVPYDQVAAVELD
                     ADGLVHLTLDPSVTPYHRLVLAGLARDPAFDHITSHRRRMRVERSITIAALAAWVPIA
                     LLLRALVADLPAVMVMGMAVSASLVLYLTRRDIATKIVLFNRTHEHVRDELLGELKAR
                     LAPGRVRAITVASEIPVPEAAPAELGEPEPDGTSLRGLFATAGVMAAMAAVAILIGKN
                     LLFSSPSDPQASGTDVREPADPVPASEPLTSASPSIVAEPVAVVPPPPPCSCDRADSP
                     LWAEGVPRMSVLAHNRPGPTSYERPSMYPEIAVVNNSSEDLKDIVMVVDFILGPRDGR
                     KARVVDKQDLFWEGRLGPGRAVKWRVRGRGDDYSVTSFVSGMLGQEAKPAPADAFYKL
                     SMTANTPSVRLHGTKMLAYLGDERVAEGLEKLRQEGREEMVESIEQIVQATRPLRVCS
                     VQANLDPADPHRFVVKACVFNASSSPMTRPWITATAKLEDSTTEARWPLPEDLPSKTG
                     LITTGTVALPAQQDGADPKATTVTVNADR"
     gene            complement(7839..9335)
                     /locus_tag="PK710_03055"
     CDS             complement(7839..9335)
                     /locus_tag="PK710_03055"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HOT08718.1"
                     /translation="MHRVLSREQIRSFDRVAIDTCQVPSVVLMENAGRGAFEVVRTLL
                     EDNDHVLVVCGSGNNGGDGFVVARHLLTAGYSVSVCLMSDPEKLRGDARLNHDAFVGL
                     GGHVVLLEDEAATVVLETELLRADLVVDALFGTGLDREIGGRYAEAIDRINATAATRV
                     ALDLPSGLDANKGTVLGTVVVADATITFGALKLGLLTPMGARLAGEIHVASLGVPSSI
                     VEQVGHEAEVLVEAPLRNIGRQIREQARILADDAVGIVVSSEEDVWAMRAAFCGALRT
                     APGRIHLMGPRPVLNAVRDCQGYAKLLELDSKSNAKGYEQLLESCDSIVFPAASEDDA
                     RWIDLARKHFDGSIVVRPSHRVGKKPKPFHASGGDVLLILGLDELGALLGADRDAVDA
                     DRFASVREARKVCHATIVLVDTQPILGIADAPLGVWGKRVDALDSDAFRALACGMMGR
                     FSSWCEGSMAALLGLNALATAVQSWWEKRDGKGNPRIEEIAEQLAEVM"
     gene            complement(9392..10129)
                     /locus_tag="PK710_03060"
     CDS             complement(9392..10129)
                     /locus_tag="PK710_03060"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006762396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOT08719.1"
                     /translation="MTIRLHVNIDHIATVRNARGTRYPDPIHAAYVCEDAGADGITAH
                     LREDRRHMVDRDIERLRESITTLLNLEMAGTDEMIGIAERIRPDCITLVPERREERTT
                     EGGLDVVGQRKQMEKVAERARKAGIRVSFFIAPEEAQLVACRDLGAEQVELHTGEYCN
                     LSGNGQLEELERLRHAARVGHELGLSIAAGHGLTRFNLPAIAAIAEIEEVNIGHAIIA
                     DSLFMGLGSAVRAMREALNRGLALREK"
     gene            10277..11668
                     /gene="allB"
                     /locus_tag="PK710_03065"
     CDS             10277..11668
                     /gene="allB"
                     /locus_tag="PK710_03065"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HOT08720.1"
                     /translation="MTHPTVFRFVNARLPTQDGPREGSLTIRDGTIACWDGPQEKGCE
                     DIDLQGAWLLPGAIDGHVHFDDPGFTHREDFASGTRAAAAGGVTTIVDMPCTSLPPVT
                     DGSSYDEKYSVVAPKAWVDFALWGGVRGNDFDRDSVSRRLDELRSRGVRSIKTYLISG
                     MDTFRDVDADQLAIVLEEAGRRGMRVGVHAEDRQDVLQATEREQAQDPAGMPASYARA
                     RSAEAEAKGVQTCIELSERTAMPLHVVHLACGRGVDLIRRAKARQVPITAETCPHYLA
                     FTVEDLERLGGLLKTAPVVKSAADRQALWEGLADGTLDMVATDHAPGAYPKEKTTGSI
                     WTDYGGVPGVELLLPYLLSEGVMRGRLSLARAMRLLSSNPAQIHSLAGKGGLQPGNDA
                     DVVVFDPRPLWTVRAKDLVTRQPYTPFEGMQWQGRVTTTYVRGRLVYDRARGFADAPR
                     GKRVEPRGSSIEE"
     gene            11686..13032
                     /gene="ssnA"
                     /locus_tag="PK710_03070"
     CDS             11686..13032
                     /gene="ssnA"
                     /locus_tag="PK710_03070"
                     /inference="COORDINATES: protein motif:HMM:NF005540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HOT08721.1"
                     /translation="MSKKLLVGPATVFTNTPGNSVVPNAGVLCANDRIERVAPFSELA
                     ASNNDATRLSTDGKVIVPGLINAHHHLYSTFSRGMPIAATTPLRNFPEILEGIWWRLD
                     RALDLEAVEAGAYPPVMDCLRWGTTTIIDHHASPNAIRGSLQALARVCDEAGIRANLC
                     YEVTDRNGDQGAVDGVQENAQFVRDCQSHRAHGALLGLHASFTVSEQTLALAARQVDP
                     ATPIHIHVAEDGADVTRTREQFGKGIIERLEAHGLLRKNSILVHGIHLTDAELDRIAA
                     AQAWLVHNPESNANNAVGHLDVQRAMARGVRVALGTDGMASNMLRAAKSAYLMIRHAR
                     KDPTVGFALPRKLLFENNVLLAREIFAEPRLGSIQAGAPADLCVIDYDPPTVLDASTF
                     DGHWVFGMTESPVWATITSGKVRMHRGEFLDLDREKISQRVLAAADGVWERLRQMP"
     gene            13050..13628
                     /locus_tag="PK710_03075"
     CDS             13050..13628
                     /locus_tag="PK710_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOT08722.1"
                     /translation="MESWKRLLVGLSTVLVVVGAVASIAMHGPNPLLHPSPMLRLSPW
                     LRESLSATLGIVFGVATVAATRWSVRHTRWARRLHRDLRPVAQQLSPGLIVLLALLSS
                     AGEELLFRSFLTPWVGIVPQALVFGILHQTRGPSRWVWALWATVIGLAFGLMYAAIGS
                     LIGPCIAHALINAINLRFLRDHEVPWDPIESR"
     gene            complement(13672..14646)
                     /locus_tag="PK710_03080"
     CDS             complement(13672..14646)
                     /locus_tag="PK710_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOT08723.1"
                     /translation="MARRIAPQAPVACWHCRTDGSVLDNDRDPGIQHRTMAVSPSDLR
                     TIPLFQDISEEHLTSLLGVFERRQHDKDEILFRAGDNPTDLLLLVRGEVALFEGDTVR
                     FRLRPIALIGELGAITGLRRYTTARITEPSEIWRIPTQSLLDFFETHGDVAFPFYHGL
                     LGIVANKVRRDARRIEEMRANIVRTQKAMKRMRDLVLESEDTSLSKPIHDTLEELIER
                     NRRWHYLVEPAKSLQARVRFDDGSMAPVVEMSDGWLLLDNSASGAHEVGAHWSAVLVL
                     PSGEIPISGTVESTDDKGIMIALDLLIREYAATLEDYLTRLHMLDFVV"
     gene            14745..15329
                     /locus_tag="PK710_03085"
     CDS             14745..15329
                     /locus_tag="PK710_03085"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOT08724.1"
                     /translation="MVEKTALGAQTFLFPMPAVVVGVMVNNRPNFMTVAWTGMACAQP
                     PMLSMAVRKQRFTYEGLTIHGLFSVNVPKVEHAEWVDYFGIVSGREEDKTSRAGIDVM
                     YRDGHEAPMIAQCPVNLMCRVEQMTTLGTHDLIVGQIVQTYVDEDCLVRGVPDVSKID
                     PLVYATGRKTYHGLGATVAKAYRVGLSLRSKKPE"
     gene            15333..16337
                     /gene="hypE"
                     /locus_tag="PK710_03090"
     CDS             15333..16337
                     /gene="hypE"
                     /locus_tag="PK710_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOT08725.1"
                     /translation="MRNDEMILLDHGGGGRASRRLIEEMLLPRLRNSALARLDDAAEL
                     SVNGYRLSFSTDSYVVDPLFFPGGDIGKLAVHGTLNDVAMGGGNPIALSVGLIMEEGL
                     PTDVLVRVIDSMAEASRQAGVPIVTGDTKVVPRGKADKLFINTAGIGIIAPDVHVSGS
                     FARPGDVVLLSGPIGSHGVAVMAERSGLEVACRSDTAALHGLVQDMLQAFAGVHCLRD
                     PTRGGLAAVLDEIAKQSSVCVHLQETSVPLGPGVQAACEILGLEPMHLANEGVLVGVV
                     PEQGATPVLQAMQAHPLGRNACAIGTVQEGEAHVILHTAIGGQRILSVPSGDLLPRIC
                     "
     gene            16362..16712
                     /locus_tag="PK710_03095"
     CDS             16362..16712
                     /locus_tag="PK710_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpyruvate tautomerase MIF-related protein"
                     /protein_id="HOT08726.1"
                     /translation="MPLLMVRTNAPVDEATIPALLQECSSQIAKLLSKPESYVMTLFD
                     RVACMTMGGKTGPTCLVEVRSVGKLTPHQTRQLSETLCSLLSKRLDVPSARIFLNFMD
                     FPGALWGHDGGVFG"
     gene            16790..16927
                     /locus_tag="PK710_03100"
     CDS             16790..16927
                     /locus_tag="PK710_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08727.1"
                     /translation="MSKPQDPPRRRREKRRRFKKEQELLLRKAEQEAKTRLVPPPEKI
                     N"
     gene            17078..18310
                     /locus_tag="PK710_03105"
     CDS             17078..18310
                     /locus_tag="PK710_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08728.1"
                     /translation="MQASGKGNTQPNPNGMPPAGLPGGAPGNALGVQLAGGAAIVAPV
                     SAFPHGVPGNALIVVPVTQPTEEFKAQLEKGPVGVTQEQMWKLLGFNGPAVQVQDDQG
                     RPIAIGLEDLLAGLEKHWKQDQEDLNRARIYAQELMKYGRLDKAEKVLGTLIASGEAT
                     GDDWLALGATQLQLKAFDKAEGTLKGAQNLMPDNPYPSLHLAKVAHEKDNKDEENEMV
                     RRAISIDRRCVDAWAYYFQFLRDRHDEAKAIEMIEQEASSGADAKNAAPFIALQGFFS
                     ANEETRERAIEFAKKGVERNPDDPLALLVLSALYGQTGNLKKVIDLLQPHEPKMMRDV
                     RLANNYFEALFQDRQIDKVTKLLNALAGSPDRQVKQFAIERSKGVAQFLQQQQQQLMA
                     ASNPATPTGPRPVGGRKD"
     gene            18492..>19359
                     /gene="amrS"
                     /locus_tag="PK710_03110"
     CDS             18492..>19359
                     /gene="amrS"
                     /locus_tag="PK710_03110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOT08729.1"
                     /translation="MDDDRDDFEVLAPGVVRARWWHRLSNGKVQCDLCPRECRLKDGQ
                     RGFCFIRENRGGQLVMTSWGRASGFCVDPIEKKPLNHFYPGSSILSFGTAGCNLGCRF
                     CQNWDLSHAMKVDRLQDQASPEQIADAAVSAGCKSVAFTYNEPIIFAEFAIATAKACR
                     RQGIKTVAVTAGYISPQARPAFFSQMDAANVDLKAFSDRFYQKLCFAHLEPVLDTLAW
                     IRNHSKTWLEITTLIVPGENDDPNEIRRLCAWCARHLGSETPLHFTAYHPAYRHKGTA
                     PTPASTLALARTI"
BASE COUNT         4181 a         5690 c         5521 g         3967 t
ORIGIN      
        1 tggggaaagc gggactgtcc atgtggcctc ccacgcgggc atcgtttatt catcggagtc
       61 gacgacgttg gatcgagaag tagcgttgga cacgtccccc gacgtttccg agggagatgg
      121 gggtgtgatt ccgtcgtatg ggctaccgag cccgccgttg ttggcagatc gttcggggca
      181 agttgcgttt gtgcgagcgg atggacgagt tggcgtgata tcgacgaacg gcacagtacg
      241 attcgcgtct cgatcgtcgt tggcaacacc ggttgacttg gcggccgcgg ggccaaacag
      301 gttcgtggtg gtttgtcgta cgggggatat ctttctttat ggtccttgag gccacggcgg
      361 aggcgggccg atccatcgct gtcgcatcca cggtgctagc ctattgacaa tgcgttgatg
      421 aacctgcggg cgcacggcga tcagcccttt ggtggtaggg gaggaggcgt cggcggggcg
      481 caatacggcg tcgtcgatca agatttgttg aacagaggtg accttggctt ccacataaat
      541 cccgatggac tcatcggaag cgcttcgcag ggcagaaaag cggcaccaac ccgaggcgtc
      601 gggttgtgga ttatcagcct ggacttgatg gaactgcgtg atgttcatgg tcatggcgga
      661 gaacatatac acggtgagtg ccgagcacgc gccgggcgtt ttggcccaaa gccagaactc
      721 gaggggcaga ttgcgcgctg ctacaccaac accgaaggcg gcgttggctt tggcggacag
      781 cgcaatgcat ctcaatccgg atcgacatcg tcctccggtt tcgacaggca cggatacttg
      841 gccgccgagc cccaacacgc gccatggcgt ctgaccttgg gccacactca tttccatgtc
      901 gccatcgagc agcaagttgt ccgtgtgggc caggtttcca aacggacttc gtagcaaaac
      961 ggatcggcga aagggtccgg cctcggtggg aggcaaaaag acatcctcgg aaaatacatc
     1021 gattatcgta gtttcggcag cggcgtccac gggtatatcg acggtgggcg cgggattggg
     1081 ctgttcgttc gtttcgcaag ccacaagggc aaaagcgatc agcgaaaggg gaaggtatcg
     1141 cttcatgggt ccagctccga acgaagccag gcgagggctc ggagatagag ggtgtctttt
     1201 ccgtgaatca gatcggactg caagggaacg acgatttcat ctggttccac accatgtccg
     1261 atgagcgctt gaccgtcgta agcgatggtg tcaccggagg ccaaactcaa gcgcatattt
     1321 ccaccgagcg ccatttgaaa gaaggacgaa aaagcgcccg cggtaggttg tggtccgaaa
     1381 atgcgaacgc gcggcgatcc tttcatacca aagggaaggt agtcgctcgc ggaaccgtca
     1441 cggtgttgaa gcagcgccac gggaagatcg agttttgctt tcgcgctacc gaccgaatag
     1501 gtgagatcag ggtagtgggt gtttttttta taacgttcga agatggcgag tccttcttcg
     1561 acggtggaag ctccctcgta atcggcccaa ggtcgatcgt gaatgaagat ggcgatatcg
     1621 agcggttcgc gcacgagttg cgtaatcgct tcaggggcgt cgatcgtgcc tccattgccc
     1681 gcgcgatgat cgaggatgac accacgagcg ttggctttga acgtttcatt ggctttgaga
     1741 aagatggggg tggtggtggg tccccaaagc gaatcccagg tgattccata taggttttcc
     1801 cctggttggg agtcttcgag caggcccacg aaaacgttcc agcccacgtt atgcgtggtt
     1861 ggatcagggc cttggacaag gtgatacgag ggccggttgt cgcaggcaac gagtggttca
     1921 tcgatttcgt cgcgtcgagg caacgaagag acctcgatca gatcgggggt atcatcgcat
     1981 gttcccgtct cggcatggca tcgcacgata gagaagtttt gtgcgtactt aggaatgaga
     2041 cctcgaatga cctcggcgaa ttccgcattg actcggtcat catccgcttg ccaccaatcc
     2101 caagagacat cgatcaacga tcgagcccat tgcaatgggt gcattccatc gatggcgacg
     2161 agacgatctc ctggtttgag gcctacgttt cggtcgggtc ccacgtgaga aacgagccaa
     2221 tcgaacgagg aggagtcgga cggccaagcg tgttgagaca gatcggcttt gccttctatg
     2281 aaacacgcgt tgaggacgta aggggactgt aagaattgga tggctccgga agcggaggta
     2341 tgccagtcgt gcaatttgcg aacggccgtg gcgaagccat gccagaactc ccacgcggac
     2401 gttgccgttc gcatcgcttc gacttgggca agagcatcag gcaagtgatc ccgtcgcgtt
     2461 tgatgaccgc cgaaaaaata ctgcaaacgg gtgaatagga agtctacgac gcgagaccgt
     2521 tctgctggcg ggggccaatg tgggacgagc gtatctccag cacgacacag gcctgcgatg
     2581 catacttcat cttttccaca ggctaggtct cttgcgccaa gacatggagc gctgggcttt
     2641 accacacctt cctcaacgat ttcaaaacca tcgatgagcg catcgcccat caaatataga
     2701 aacgctgcgc tcgttgttag gccatcgatg gtcaccggcg cacttgccat ctccacccaa
     2761 ccatcgctgg tgcatcgacc ggttgccgat agatccgcca cggagcgttg cagcgggtcc
     2821 gcggcatcat ccacgacgaa ggtggctgcg agccaaccgg atcgaatcca aagtcgaatg
     2881 cgataagacg cgagaagggg agggagggtc acgggcaaac gaactccctc tgcccaccat
     2941 tcgggagata cggctcccac acgatttcct tcgagtgcgc cagggtcgtt caaggtaaat
     3001 agatttttga tggcgttggg atcgggttga gcaggattcc acgaagggag ggactcgaag
     3061 ctttccgtat gaaccgcgcg aggatcgaac gtgtagttgc cttggggatc gaaatgggcg
     3121 aacgcgttgg tgggcagaaa taggcccatc aagggtgcga accaagccca aagcgaacgt
     3181 ggattcatgg gttgccaact ccgaaaaatc tagggaaggg tcgagtcttg tcgccagcgc
     3241 ggactttggc ggcaaagcca ggggagcgtc aaggtaaagc ccggagtcca ggaagcgatc
     3301 attcgtggtc cgatacaccc ataattattt gatattattc ataaaaatat tttatcaaaa
     3361 gattttttgg cgacggcgtt tcgttcatca gcgatttatg tagcgttcga ctcgcgctcc
     3421 attgtagatg gaagcacttg agtgtgcgcg gggcctgtga gcacgcgcaa gtccagcccg
     3481 ccgtgtcggg caccggagtc tagctgcccg tagatggaga tattgcgatg ttgagactac
     3541 gtacgcttgg aattgcgatg gcactggtgt tgggcgcctc tggcgtcatg ctgaccacgg
     3601 gttgcgggga ggccgtgttg caggtgggag tcaatcctcc gcctggaccg cctccagcgc
     3661 cacctcctcc tccggagcct gagcccgttg cggctcctga gcctccgccc gagcctcctc
     3721 cgctggtgga ggtcaagggt gacgaaatca agatcaatga caagattaac ttcgcaaaga
     3781 actcggcgga gatcgaatcg tcttccaaca gcctcatcga aaacatcgcc aacgtgatga
     3841 agaagcatgc ggatatcgac ttcgtggaag tcgccggcca tgcttctcga gaaggcgacg
     3901 agttctacaa caagaacttg acgcaacgtc gagccaaggc cgtcatggac gcgttggttg
     3961 cgctgggcgt cgagaaaaac cgtatgcgcg ccgtgggtta cggcttctat tgtgagctgc
     4021 agcctgacaa ccacgaggcc aatcgtcgcg tggaattcaa aatccttcgt cgcgcgggca
     4081 aggatacttc ggtcaagggc ggcggatgcg aagctgccga caagaagggt ttgacggcaa
     4141 agccgatccc ggcggatgca ccgaaaaccg agaagtagta tttccgtttc ggattctgat
     4201 gacacccatc acgcggcatt ttgctgcgag gggggtgttg tatccaccaa tcccaatccc
     4261 tagaatcgtc catccattcg cacgcctgcc cagcctcctt gtggtggtgg ctgcgggctg
     4321 aacgacaatc ccggttgatc gggctgtgtg agcgtattgt ctggttgcga gcccccactg
     4381 aatatgaaca acaatgcccc ggtgacgatt cccgccgcgc ctacgcccag cgccacgttc
     4441 gcgaacaacg tatcccgctt tcccgtgtcg tacgtttttt tcatcgagga ggggcaagcc
     4501 gcgcgcccac ttccacaagc gtcatcgaga tcggaaatag ctttcgagcg ctgcaccatg
     4561 aaataacccg aggcgccgag tccaacgaca ccgacaccgg ttgcgatcca ggcaagggta
     4621 ttcgatcctt ctcgagaatc gatttcgacg gaggacgagg aagatgaatc tgtacgttct
     4681 ggcgtgtttt gtgggggaat cggagcagcc gctcccggcg ctcgtttgat catcttgatt
     4741 tcgatcgtgg ctttctcgcc ttccgcgagt cggacttctt ggcgaaacgg aaggtacccc
     4801 gcggcctgag ccacgaccac gcgcgttccg ggatcgaaag gcatgggttg accgatcgcg
     4861 gactcaccaa gaggttttct atccacgagc acggttgcgg tagcggcacc atcgcctcga
     4921 tgaatgatca atctgggaat tcgaggttcc acccgaccga tcgcttcttg ggcttcggca
     4981 atcacatcgc tggccgcgga agcttgcgca tcggcgagag caattcggta ctcgcccaac
     5041 gccatcacca gcttgcccaa cttctcgtgg caataaccaa tatagaatcc cacctgaggc
     5101 gtgcgtttga aggttgcgac acgctccaac ttgccgaggg cagtcgtata gtcccccgct
     5161 gccacgtgcg cgagagcctc ttgaaacaag gttcgtgctt cttgtttggt gggaggctcc
     5221 gccccaacag agccgctcgt ggtcatggta gccgagccga tagccacaca agcacatagc
     5281 acccacggat agcttcgcgc atgcaacatg gtcgcaggat agcgcgcgcc tcggattctg
     5341 gggagtccat gtgacttgca ttccgctgga tctttcacgt tctcgcacta cactactttc
     5401 cgcctgaatc atgaaactcg cccccgttct tccgttgaaa aaaaaatcgc gcacgcgaca
     5461 acgcgtcgaa cgggttcttc tcttcattag ccgcaaaaaa aaggcaaaat ccgtaaacca
     5521 ggagccaccg aaagcttcgg cgcgcccacc tcgcctctcc ccttccgtac gtccgacccg
     5581 cgcttccacc gccccaccat ccttgctcga cgcgagcgcg gccttgatgg gggataccga
     5641 cgttcctttc gcaccgtcgt cggcaactct ccccaaaaaa acccgtaaaa aacctaagaa
     5701 aaatcctttt ctttccactc ccgtaccgga aacctttcgc ccgccggcgc ccacacaaca
     5761 agccccttcg gtgctttcag cgcccttggc cgctgctccg aaaaccaaag aaaaccaact
     5821 ccctgctgcc ccaccctcgt tcccttccgt cgcctctcgg atcgaagccg aggcgcaaac
     5881 aatatccgaa cgcaaaccac cagcacggcc ttcccctccc accccctctt atgcgccggt
     5941 ttcccgtgat gtgtcggttc aacacacacc ccggtctacc ttccgggaat cgtattcccg
     6001 tcaggattca ccggtcttgc ctcggtacgg atcaaccacc gtccccgtgc gcgccgtggc
     6061 catcgcccac gaaaaaagca acgcggtagg caccatcgaa ctgtcctgcg tcgcacacgg
     6121 attacgcatc caattcgtcc gaatcagcag ctatacggaa ggattcgttc cttaccccgc
     6181 cacatcctcc gagcgtatta ccgtccctta cgatcaagtg gccgccgtcg aattggatgc
     6241 cgacggattg gttcacctta cgcttgaccc ttccgttaca ccataccatc gcctggtgct
     6301 cgcgggtctc gcgcgcgacc cagccttcga ccacatcaca agccatcgcc gtcggatgcg
     6361 ggtcgaacgc tccattacca tcgctgctct tgccgcatgg gtgcccatcg cattgctgct
     6421 gcgagcgctg gtggccgact tgcctgccgt gatggtgatg ggaatggccg taagtgccag
     6481 cctcgtgctt tacctgacac gtcgcgatat cgcgaccaag atcgtgttgt tcaaccgaac
     6541 ccacgagcac gtgcgtgatg aactccttgg tgaattgaaa gcgcgtcttg caccgggccg
     6601 cgtgcgagcc atcacggtgg catcggagat tcccgtaccg gaggcagccc ccgccgaatt
     6661 gggggagccg gaacccgatg gcaccagtct acgcggatta tttgccaccg cgggggtcat
     6721 ggcagcgatg gcggccgtgg ctattctcat cggcaaaaac ttgttgttct cctcgccgtc
     6781 ggatccgcaa gccagtggga cagacgtgcg tgaacccgcg gatccagtgc cagcttccga
     6841 gcctttgacc agcgcttcgc cttccatcgt ggccgaaccc gtggctgtcg tgcctccacc
     6901 gcctccttgc tcgtgcgacc gcgccgattc accgctgtgg gccgagggcg taccacgaat
     6961 gtccgtgctc gctcacaacc gaccaggtcc cacttcttac gaacgacctt cgatgtatcc
     7021 cgagatcgct gtcgtgaaca actcgtcgga ggacttgaag gatatcgtca tggtggtgga
     7081 cttcattctc ggtccaaggg acggtcgcaa agcgcgggtg gtcgacaaac aagacttgtt
     7141 ctgggagggg cgtcttgggc cagggcgtgc ggtcaagtgg cgcgtgcgcg gccgcgggga
     7201 cgattattcc gttacctctt tcgtcagcgg catgctcgga caagaagcca agccggctcc
     7261 cgccgacgcc ttctacaaac tgtcgatgac ggccaatacg ccgagcgtgc gtttgcatgg
     7321 tacgaaaatg ctcgcgtatt tgggcgacga gcgggtggcg gaaggactcg aaaaacttcg
     7381 gcaagaaggc cgagaggaga tggtggagtc gattgagcaa atcgtacaag ccacgcgacc
     7441 ccttcgggtc tgctccgtgc aagcgaatct cgaccccgcg gatccccatc gattcgtcgt
     7501 caaggcctgc gtattcaacg ctagttcctc acccatgact cgaccgtgga ttacggctac
     7561 cgccaagctc gaggattcca ccaccgaagc cagatggccc ttaccagaag acttgccgtc
     7621 gaaaactggc ttgatcacca ccggaacggt cgcgcttccc gcccagcaag atggggccga
     7681 tcccaaggcg acaaccgtta ctgtgaatgc cgatcggtga cggtccacag cggaacggca
     7741 acgtgtcgga agcgatctcc gttgtcccgg cgaatacaat ataactaatt gaatttgttg
     7801 atataatatt cggtttcacc gactcgggat cgcccgggtt acatcacctc cgcgagttgt
     7861 tcggcgatct cttcaatccg tggattgcct ttcccatccc gcttttccca ccaagattgc
     7921 acagccgtgg cgagcgcatt cagtccgagc aaagcggcca tgctgccttc gcaccaactc
     7981 gagaaccgtc ccatcatgcc gcaagcgagt gcgcgaaagg catccgagtc gagcgcatcg
     8041 actcgtttgc cccatacgcc caagggagca tcggcaatcc caaggattgg ctgggtatcg
     8101 acaagcacaa tggtggcatg gcaaaccttt ctcgcttccc gaacggaagc aaatcgatcg
     8161 gcatccaccg catcacggtc tgcgccaagc aacgcgccaa gctcatccaa tccaaggatc
     8221 aaaaggacat ctccacccga tgcatgaaag ggtttcggct ttttccccac gcgatgggag
     8281 gggcgaacca cgatcgagcc atcgaagtgt ttgcgagcga gatcgatcca ccgagcatcg
     8341 tcttccgaag ccgcgggaaa gactatggaa tcacaagatt cgaggagttg ttcatatcct
     8401 ttggcattgc ttttcgaatc caattccaga agtttcgcgt agccctggca atcgcgaacg
     8461 gcgttcaata ccggacgagg acccatcagg tggatgcggc caggagctgt tcgcaacgcg
     8521 ccacaaaacg ccgcgcgcat cgcccacaca tcttcttccg acgaaacgac gatccccacc
     8581 gcatcatcgg cgagaatgcg ggcttgttcc cggatttgtc gcccgatatt tcgtaaaggc
     8641 gcctcgacga gcacttccgc ttcatgcccg acttgttcga cgatcgagga tggcacgccg
     8701 agggaagcca cgtggatctc accggcgaga cgggcgccca tgggtgtcaa caagccgagt
     8761 ttgagagcgc caaacgtgat ggtggcgtcg gcgaccacga cggttccgag cacggttccc
     8821 ttgttggcat ccagtccact gggcaaatcg agcgccacgc gtgtggctgc cgtggcgttg
     8881 atgcgatcga tggcttccgc gtatcgacca ccaatttcac gatcgagacc cgtgccgaaa
     8941 agggcatcca ccaccaggtc ggcgcgtagc aattccgttt ccaacaccac ggtcgctgct
     9001 tcatcttcca agagaaccac atgtcccccc aacccgacga aagcatcatg gttgagccgc
     9061 gcgtcgcccc gcagtttttc gggatcggac atcaaacaaa ccgaaacgct gtaacccgcc
     9121 gtaagcaaat gacgagcaac cacgaaaccg tcgcctccgt tgttgccgga tccgcaaacg
     9181 acgagcacgt gatcgttgtc ctcgagcaac gtgcgaacca cttcgaaagc cccccgcccc
     9241 gcgttctcca tgagaacgac gctgggcacc tggcaggtgt cgatcgccac gcggtcaaag
     9301 gagcggattt gttcacggga aagaacgcgg tgcatgatga ccctcatggg ctgttcggga
     9361 aagcctttcg tcaaaatcag cgacaaggac cctacttttc gcgaagagcc agaccacgat
     9421 tcagggcttc gcgcatggcg cgaaccgccg agccgagccc catgaacagg gaatcggcaa
     9481 tgatggcgtg gccgatattg acttcttcga tctcggcaat ggcggcaatg gccgggagat
     9541 tgaatcgagt caacccatga cctgccgcaa tggataaccc cagttcgtgc ccaactcgag
     9601 cggcatgccg caatcgctcg agttcctcta gctgtccgtt tccggataga ttgcagtact
     9661 cccccgtatg aagctccact tgctcggcac caagatcacg gcaagccacg agttgcgcct
     9721 cttccggagc aatgaaaaaa ctcacgcgaa tccccgcctt gcgcgcccgc tccgccacct
     9781 tctccatctg cttgcgttga cccaccacat cgagtccccc ctcggtcgtg cgttcttcac
     9841 gacgctccgg aaccagcgta atgcaatcgg gacgaattcg ttccgcaatc ccgatcattt
     9901 cgtccgttcc tgccatctcc aagttcaaca acgtcgttat gctctcccgc aatcgttcga
     9961 tatcccgatc caccatgtgc cgacgatcct ctcgaaggtg ggccgtgatg ccgtcggcgc
    10021 cagcgtcttc gcatacataa gccgcgtgaa tcggatccgg atagcgtgtc ccacgcgcgt
    10081 tgcggacagt agcgatatgg tcaatgttga cgtgcagtcg aatcgtcatg gggcgagtgt
    10141 agccgcgcga agacctcgtg agtaccctcc gcgttcgaat gggtgacgac acctcggatc
    10201 aggctggttt ggcttgcgaa atccgcatat tgtccccatt tcaggacatg gagccctgct
    10261 gcgcttgcga gaaatcatga ctcatccaac tgtttttcga ttcgtcaacg ctcgtctacc
    10321 gacccaagac ggaccacgag aagggagttt gaccattcgc gatggtacga tcgcgtgttg
    10381 ggatggcccc caggaaaagg gttgcgaaga tattgacctg cagggcgcgt ggcttcttcc
    10441 gggcgctatc gatggtcacg ttcatttcga cgacccgggg tttacccacc gggaggattt
    10501 tgcctccgga acgcgcgcgg cggctgccgg tggcgtcacc accattgtcg atatgccgtg
    10561 tacgtcgttg cctccggtga cggacggaag ctcttacgat gaaaaatatt ccgtggtggc
    10621 gcccaaagca tgggtagatt ttgccttgtg gggaggcgtg cggggcaatg atttcgaccg
    10681 cgattcggtg agccgccgtc tcgacgaact tcgatcgcgc ggtgtccgat cgatcaaaac
    10741 ctatctgatt tccggaatgg atacgttccg tgatgtggat gcggatcagc ttgcgatcgt
    10801 attggaggaa gctggtcgtc gcgggatgcg cgtgggagtc catgcggaag accgtcaaga
    10861 cgtattgcaa gccaccgagc gcgaacaagc gcaagatcca gccggaatgc cagcttcgta
    10921 tgcaagagca cgcagtgccg aggcggaagc caaaggagtt caaacgtgta tcgagctatc
    10981 ggagcgaacg gcaatgccgc ttcacgtagt gcatctggct tgcggtcgag gagtcgatct
    11041 cattcgacgt gccaaggcac gacaggtccc gatcacggcg gaaacctgcc cccattatct
    11101 ggcgtttacc gtcgaggatc tcgagcgttt gggaggactg ctcaaaaccg cgccggtcgt
    11161 caaaagtgcc gccgatcgac aagctctttg ggaaggactg gcggatggca cgctcgacat
    11221 ggtagccacg gaccatgcgc ctggagccta tccgaaggaa aaaacaaccg gatccatctg
    11281 gaccgattat gggggcgtgc ctggagtcga acttcttttg ccgtatctgc taagcgaagg
    11341 ggtgatgcga ggccgcctct ctctggcgcg agcgatgagg ctattgtcct ccaatccagc
    11401 acaaattcat tcgttggccg gaaaaggggg actacagccg ggcaacgatg cggatgtcgt
    11461 ggttttcgat ccgcgtcccc tatggactgt gcgcgccaaa gacctcgtga cccgccagcc
    11521 ctatacaccg ttcgaaggta tgcaatggca aggtcgcgtg accaccacct atgtgcgcgg
    11581 ccgtctggta tacgataggg cccgaggttt tgccgatgca ccccgtggca aaagggtcga
    11641 acctcgtggc tcgtcgatcg aagaatgaaa agcgtggagt caaagatgtc caaaaagctg
    11701 cttgtaggcc cggccaccgt attcaccaat acccctggca attccgtcgt accgaatgcc
    11761 ggcgtcttgt gcgcaaacga tcggatcgag cgggtggcac ccttttccga gttggcggcg
    11821 agtaacaacg atgccacgcg attgtccacg gacggcaagg tgatcgtacc gggactcatc
    11881 aatgcccatc atcatttgta ttccacgttt tctcgcggca tgccgattgc cgccacgaca
    11941 ccgctgcgca acttcccaga aattctcgaa ggaatttggt ggcgtttgga tcgagccctc
    12001 gatctcgaag cggtagaggc cggcgcctat cccccggtga tggattgcct ccgttgggga
    12061 accacgacca tcatcgatca ccatgcttcc cccaatgcca tccgaggatc gctacaagcg
    12121 ttggcgcgag tatgcgatga agcggggatt cgcgcgaatc tctgctacga agtgacggat
    12181 cgaaacgggg atcagggagc cgtcgatggc gtgcaagaaa acgcgcaatt cgtgcgagat
    12241 tgtcaaagcc accgagccca cggggccctg ctcggcttgc atgcatcgtt caccgtttcc
    12301 gaacaaacgc ttgctcttgc ggctcgacag gtggaccccg ctacacccat tcatatccat
    12361 gtggccgagg atggggccga cgtcacccga acgcgggaac agtttggaaa aggcatcatc
    12421 gaacggctcg aagcccacgg actactccga aaaaatagca ttttggtcca cggtatccat
    12481 ttgaccgatg ccgaactcga tcgcattgcg gccgcacaag cttggctcgt tcacaatccc
    12541 gaatcgaacg cgaacaacgc cgtgggccac ttggatgtgc aacgcgccat ggcacgcggt
    12601 gttcgcgttg ccctcggtac cgatggaatg gcttcgaaca tgctccgagc cgccaagtcc
    12661 gcgtacttga tgatccgaca tgcacgcaaa gatcccacgg taggatttgc gcttcctcga
    12721 aaattgttgt ttgaaaacaa tgtgttactc gctcgagaaa tattcgcaga gccacggctc
    12781 ggttcgattc aagcaggagc acccgcggat ttatgcgtga tcgattacga tccgcccacc
    12841 gttctcgacg caagcacctt tgatggtcat tgggtgtttg gaatgacgga atcaccggtg
    12901 tgggcgacga taacgagcgg aaaggttcgc atgcatcgcg gtgagttttt ggatctggat
    12961 cgagagaaga tttcgcaacg agtccttgcc gcggccgatg gggtgtggga gcgactgcgc
    13021 cagatgccct gaggatattc atcacaacca tggagagttg gaagcggctt ctggtgggat
    13081 tgtccaccgt attggtcgtg gtgggcgcgg tggcgtcgat agcgatgcac ggacccaatc
    13141 cgctgctcca cccatctccc atgcttcgac tttcgccatg gttacgcgaa agcctgtcgg
    13201 cgaccctcgg aatcgtattc ggggtagcca ccgtggctgc cacgcgatgg tcggtccggc
    13261 acacgcgatg ggcgcgacgg cttcaccgtg acttgcggcc cgtggcccaa cagctctcac
    13321 cagggttgat tgtcttgttg gcattgttgt cgagcgcggg ggaggagctt ctgtttcgct
    13381 cgtttcttac gccctgggtc ggaatcgttc cgcaagcctt ggtatttggt atcctgcacc
    13441 aaactcgtgg acccagccga tgggtatggg ccctgtgggc aacggtgata ggtcttgctt
    13501 tcgggctcat gtacgcggcc atcggttcac tcatcggacc gtgcatcgcc catgcgttga
    13561 tcaacgcgat caatcttcgc tttcttcgcg atcacgaagt cccttgggat cccatcgaat
    13621 cgaggtgaac gcggcaggcg atcgacacac gcggtcgtgc gcgatgcaag atcagaccac
    13681 gaagtcgagc atgtgaagac gtgtcagata gtcttccaac gtggcggcat attcgcgaat
    13741 caatagatcg agtgcgatca tgatgccctt gtcatccgta ctttctacgg ttccgctaat
    13801 ggggatctcg cctgacggaa gaacgagcac ggcgctccaa tgggcaccga cctcgtgcgc
    13861 tcccgatgcg gaattgtcga gaagcagcca gccgtcggac atctccacca cgggggccat
    13921 ggagccatca tcgaaacgaa ctcgggcttg cagtgatttt gctggttcga cgaggtaatg
    13981 ccagcgacga ttgcgttcga tcagttcttc gagggtatcg tggatgggtt tgctcaacga
    14041 cgtgtcttcc gattcgagga ccaggtcgcg cattcgtttc atggcttttt gggtgcggac
    14101 gatattggcg cgcatctctt cgatacggcg cgcatctcga cgaaccttat tggcaacgat
    14161 tccgagcagg ccgtggtaga aaggaaacgc gacatctccg tgagtttcga aaaaatcgag
    14221 caacgattgg gtggggattc gccatatttc ggagggttcg gtgatgcgtg cggtcgtata
    14281 acgtcgcaga ccggtaatcg cccccaattc accgatgagg gcgatgggcc gcaaacggaa
    14341 gcggacggta tcgccctcga atagggcgac ttctcctcgt acgagcaaaa gtagatccgt
    14401 ggggttatca ccggcccgaa agaggatctc gtctttgtcg tgctggcgtc gttcgaacac
    14461 gccgagcaac gaggtgagat gctcctcgga aatgtcttga aaaagaggga tcgtgcgcag
    14521 atcagaagga gaaaccgcca tggttcggtg ttgtatccca ggatcccgat cgttgtcgag
    14581 taccgagcca tcggtgcgac aatgccagca ggccacgggt gcctggggcg cgatccgacg
    14641 agccatcggt ggttgccaca gcgcagcggg gaagtccaag cgggaaaacg cccaaaccat
    14701 ggagttcgga accccaagcg tatcaatgct gctacggtca gcccatggtt gaaaagacag
    14761 cactgggtgc ccaaaccttc ctatttccca tgcccgcggt ggtcgtgggc gtcatggtta
    14821 acaaccgacc caacttcatg accgtcgctt ggactggcat ggcatgcgcg caacctccca
    14881 tgctcagtat ggcggtgcga aaacagcgct tcacctacga agggctcacc attcacggac
    14941 ttttctccgt caatgttccc aaggtcgagc atgccgaatg ggtcgattac ttcggcatcg
    15001 tttctggtcg cgaagaagac aaaacctcac gagccggcat cgacgtcatg taccgcgatg
    15061 gccatgaagc acccatgatc gcccaatgtc cggtgaatct gatgtgtcgc gtcgaacaga
    15121 tgacaacgct cggaacccac gacttgatcg tgggacagat cgttcagacc tatgtggacg
    15181 aggattgttt ggtccggggg gtgcccgacg tatccaagat cgatcccctg gtatatgcga
    15241 cgggtcgtaa gacgtatcac ggtctggggg ctaccgtggc gaaagcgtat cgggtgggtc
    15301 tttccctgcg atccaagaaa ccggagtgat tcatgcgcaa cgacgaaatg atactgctcg
    15361 atcatggcgg aggaggaaga gcgtcgcgtc ggctgatcga agaaatgctg cttcctcgtt
    15421 tgagaaattc cgcgttggcg cgtctcgatg atgcggcaga actatccgta aacggatata
    15481 gactatcctt tagcacggac tcctatgtcg tcgatccatt gttttttcca ggtggagaca
    15541 ttggaaagct ggcggtccat gggacgctca acgatgtggc gatgggagga ggcaatccca
    15601 tagccttgag cgtgggcctg atcatggagg aagggcttcc caccgatgtt ctcgtgcgag
    15661 tcatcgattc gatggcggaa gcttcgcgac aagcaggtgt cccgatcgtg acaggcgata
    15721 cgaaagtggt gccacgggga aaggcggaca aactattcat caataccgcg ggcatcggca
    15781 tcatcgcgcc cgatgtgcat gtcagcggaa gcttcgcgcg tccgggcgat gtcgtgctgc
    15841 tcagcggtcc catcggatct cacggcgtgg ccgtcatggc agaacgttcc ggactcgagg
    15901 tggcgtgccg aagcgacacc gccgcgcttc atggcctcgt gcaggatatg ctgcaagcgt
    15961 ttgccggcgt gcattgctta cgcgatccga cgcgaggagg actcgccgcc gtgctcgacg
    16021 aaatcgcaaa acagtcgtcg gtatgcgtgc atcttcaaga aacgtccgta ccgcttgggc
    16081 ctggggtgca agccgcatgc gaaatccttg gcctcgaacc gatgcatctg gcgaatgaag
    16141 gcgtgctcgt aggcgtggtc cccgaacaag gcgcgacgcc tgtccttcaa gcgatgcaag
    16201 cccatccttt gggccgtaac gcatgcgcca tcggtaccgt gcaagaaggc gaagcccacg
    16261 tcatcctgca taccgccatc ggcggccaac gcatcctatc cgtcccctcc ggcgacttgc
    16321 tccctcgcat ttgctaacca cttcgtctat caacatcacc catgcctctg ctcatggtcc
    16381 gaaccaatgc gcccgtcgac gaagcaacca ttcccgcgct gctgcaggag tgctcgtcgc
    16441 aaatagccaa actcttgagc aagcccgaaa gctacgtcat gacgctcttc gaccgcgtag
    16501 cctgtatgac gatgggcgga aaaaccggcc ccacctgtct cgtcgaggta cgcagcgtgg
    16561 gaaagctgac gccccaccag acccgtcagc tcagcgaaac cctttgctcc cttctttcca
    16621 agcgtctgga tgtcccttcc gcccgtattt ttctgaactt catggacttt cccggcgccc
    16681 tatgggggca cgatggggga gttttcggct agatcctccc cttcgaaaaa ttccctccga
    16741 tcacaagatt gatcttccct cgcgcgggca ttgcgctaaa tgaggggcca tgtccaaacc
    16801 tcaagatccc ccgcgccgac gtcgcgaaaa gcgacgccgt ttcaagaaag agcaagagct
    16861 attgcttcga aaagccgagc aggaggcgaa aaccagactg gtgccgcccc cggaaaaaat
    16921 caattaatcc agtagcttat gacttaatcg cccaaggccc gccgggctgg ttcgcgagca
    16981 ccaagacacc caatccccgt cgatgacggt gcaagctgga tgtccgcgtg atagcctctc
    17041 gcgccccgac gcgatcgtct gacacgagga gaaatcgatg caagcttcag gcaaaggcaa
    17101 cacacaaccc aacccgaatg gaatgccacc ggccggcttg ccgggaggag cacctggaaa
    17161 cgccctgggg gtccaactcg ccggaggagc cgccatcgtc gcgcccgtct ccgctttccc
    17221 ccatggggtt cccggcaacg cgctgatcgt ggtgcccgtc acccagccca ccgaagagtt
    17281 caaagcacag ctcgaaaaag gtcccgtggg cgtgacccaa gagcaaatgt ggaagttgct
    17341 cggcttcaac ggtccggcgg ttcaggtgca ggacgatcaa ggacgtccca tcgccatcgg
    17401 cctcgaagat ctgcttgccg gactcgagaa acattggaag caagatcaag aagacctcaa
    17461 ccgcgcccgc atctatgccc aggaactcat gaaatacgga cgcctcgaca aagccgaaaa
    17521 agtcctcggc actctcatcg cctcgggaga agcgacgggc gacgattggc tcgcgttggg
    17581 tgccacccag ctccagctaa aggccttcga caaagccgag ggaaccctca agggcgcgca
    17641 gaacttgatg cccgacaatc cctatccctc gttgcacctc gccaaagtag cccacgagaa
    17701 ggacaacaaa gacgaagaaa acgaaatggt tcgtagagcc atttccatcg atcgccgctg
    17761 tgtcgatgcc tgggcctatt acttccaatt tttgcgcgat cggcacgatg aagccaaggc
    17821 catcgagatg atcgagcagg aggcctccag cggtgccgat gcgaaaaacg ccgcgccttt
    17881 catcgccctt caaggctttt tctcggcaaa cgaagaaacg agggaacgcg ccatcgaatt
    17941 tgccaaaaaa ggtgtcgagc gcaaccccga tgatcccctc gcgcttctcg tcctctccgc
    18001 gctgtacgga cagacgggca acttgaaaaa agtcatcgac ttgctgcagc cgcatgaacc
    18061 gaagatgatg cgcgacgttc gcctggcgaa taattacttc gaagccttgt tccaagatcg
    18121 ccagatcgac aaggtgacca agttgctcaa cgcgctcgcc ggttcaccgg atcgtcaagt
    18181 caaacaattc gcgatcgagc gttccaaagg cgtggcgcaa ttccttcagc aacaacaaca
    18241 gcagctcatg gccgcgagca atcccgcgac acccaccggc cctcgaccgg taggtggccg
    18301 aaaagattga ttaaatctcc ctgcgccccc cttttcttcc cccttcccct tcccacatcg
    18361 cttcgatcct ttcgccctcg tcatcgctat ccttgcataa gctcctgctt ccacccgcca
    18421 cccgatcgaa cgagggaggc gttcaccaca cccgtgattg cagaccaatc tcactcgcgt
    18481 agactaagcg cgtggacgat gatcgcgatg acttcgaggt gttggctccg ggtgtcgtgc
    18541 gggcgcgatg gtggcatcgg ctttccaacg gcaaagtgca atgcgattta tgcccccggg
    18601 aatgccggtt gaaagatggg caacgcggtt tttgtttcat tcgagagaat cgtggtggcc
    18661 agctcgtgat gacgagttgg ggtcgggcct ccgggttttg tgtcgacccc atcgaaaaaa
    18721 aacctctcaa ccatttttat cccggctcga gcatcctttc ctttggcacc gcaggatgca
    18781 atctcggctg tcgtttctgc caaaactggg acctttccca cgccatgaag gtggatcgtc
    18841 ttcaagacca ggcttctccc gaacagatcg ccgatgctgc cgtatccgct ggatgcaaaa
    18901 gcgtcgcttt cacctacaac gagccaatca tttttgccga attcgccatc gccaccgcca
    18961 aagcgtgtcg tcgacaaggg atcaaaaccg tggccgtcac cgcgggctat atctcacccc
    19021 aagcgcgtcc cgccttcttc tcccagatgg acgccgccaa tgtcgacctc aaagcgttct
    19081 ccgatcgatt ctaccaaaaa ttatgcttcg ctcatctcga gcccgtgctc gatacgctcg
    19141 cgtggattcg caatcattcc aagacgtggc tcgaaatcac gaccttgatc gttccgggcg
    19201 aaaacgatga ccccaacgaa atacgtcgct tatgcgcgtg gtgcgcacgc catctcggat
    19261 ccgaaacgcc tctgcacttc accgcgtatc atcccgcgta tcgccacaaa ggcacggccc
    19321 ccacaccggc atcgaccctc gctcttgctc gaaccatcg
//
LOCUS       DAOSOR010000032        19320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_2834_length_19320_cov_4.710615, whole genome shotgun sequence.
ACCESSION   DAOSOR010000032 DAOSOR010000000
VERSION     DAOSOR010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 19320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19320
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2834_length_19320_cov_4.710615"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PK710_03115"
     CDS             complement(<1..557)
                     /locus_tag="PK710_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08730.1"
                     /translation="MAVGLLAPGARANESWSLVHSEVNRGNAVMSLSAIDGNTAWAVG
                     ISKQGSSQTPAGWRTINGTDWGYMSLPSGTGGAMQYTIPTQVAFLDANTGWMSGMRVA
                     PMTGQENLIWRTTSAGMGWDELYQAPEEILHLQVTHDWAMFAVGGSTVVRTQDGATFV
                     ESKPTLPSGMGLVGIQMFNKDCGYAV"
     gene            complement(734..1846)
                     /gene="hypD"
                     /locus_tag="PK710_03120"
     CDS             complement(734..1846)
                     /gene="hypD"
                     /locus_tag="PK710_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOT08731.1"
                     /translation="MKNIPFISAFRDPAPVRGLVARIHEQAKPLAKATFMEVCGTHTM
                     SLYRHGIRSLLPKHVKMLSGPGCPVCVTPHAYLDRAIALSRQPGFVITTFGDMMRVPG
                     STSSLQRERAKGADIRVVYSTLDAVDMAEREPNRSFVFLGVGFETTSPTIAAAVVSAQ
                     HRGIKNFFIASAAKRVAPALDVIVQDERLQLDGLLCPGHVSAILGTSVYEPLANDHGV
                     PCVIAGFEALDVLQGISMLLEQVVDRQAKVQNAYRRAVEPQGNRVALALLDRVFEPVD
                     STWRGLGSLPASGLRLRSAFRDFDADRFEVEVEPTREPRGCACGDILRGILTPFECKL
                     FGTVCTPDSPQGACMVSSEGTCAAAFRYGQTPAPTS"
     gene            complement(1843..2073)
                     /locus_tag="PK710_03125"
     CDS             complement(1843..2073)
                     /locus_tag="PK710_03125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOT08732.1"
                     /translation="MCLAVPMQVLRVSDDRAIAMVDGVEREIGTALVPDVQPGEYVIV
                     HAGFAIQKLSAQEAQETLSMLQLLSEEPLEPR"
     gene            2170..2832
                     /locus_tag="PK710_03130"
     CDS             2170..2832
                     /locus_tag="PK710_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08733.1"
                     /translation="MKMTLRLGIACSFGWLLNACPSDQAPLGTGELGKGDLRIVCADD
                     DAVCASGATSLDAAFPIAVGASIQVVYDGPVPTSPTGGPSTMTIFSASPAMLSKDEAS
                     FVAWMPGVVALLARTQEGTVADFVHLTLASVQRLELDSSPEPLVVGGKKVWKVAPLGA
                     DGAVLAGSLSYTWEVEGTTVEIVESAGRSVQLSGKQPGTAILRVKTGGVVGEVTLTVE
                     AP"
     gene            2829..3215
                     /locus_tag="PK710_03135"
     CDS             2829..3215
                     /locus_tag="PK710_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08734.1"
                     /translation="MSRLVWLVPLWCISLLGGCSSDEGFDLERSTDAPGEVSVTASSV
                     TLPQGVAVGVRLRLHRSEDPENGEFTTAMRSTNPQIVQVWPTTERNTFVLCGASEGVA
                     SVTVLVEAQDDRTIAVVVDPPLRVTP"
     gene            3212..4132
                     /locus_tag="PK710_03140"
     CDS             3212..4132
                     /locus_tag="PK710_03140"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT08735.1"
                     /translation="MSRRQRARWLLGILLFAVGCSGEDEGSSWSWGPADGGDEGLADQ
                     VVDTAMEAADSGEMGDGQPDDGKVEAHEDVHGDVPDEQPDDAQEEASEDAFDAGPCPE
                     DMVLVEQTCIDRFEAPNRQGASPLVMFHFLEAESWCETRGKRLCFDDEWTRACGGPQG
                     HSYPYGDSHVSGECTDDKAWRAYEQQLLNGWPWSIATDAIESLEALLEAVRSKGAGAK
                     AAADHVAWLYQAEPSGSKQGCTAGEGTYDLAGNVEEWTRRRSGGTTGYHGNLKGRYWA
                     ESRTCQQSVTTHGDTFRFYEIGFRCCKDVE"
     gene            complement(4235..4852)
                     /locus_tag="PK710_03145"
     CDS             complement(4235..4852)
                     /locus_tag="PK710_03145"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001886.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HOT08736.1"
                     /translation="MSFPKPFYRWRPHPWHGLTLGPQPPITVHAFIEITPFDTVKFEV
                     DKETGYLRVDRPQRSSSQPPALYGFIPRTLCAQRVQALSPNSKRGDGDPLDICVLSER
                     PISRGEILVNARVIGALQMIDGEEADDKIIAVLEKDNVWGMAQDIRDVPQVLVERLRH
                     YFLTYKLIPGETSNVVEIERVLGAKEAHAIIDAAHQDYDEMYGSG"
     gene            complement(4931..7609)
                     /gene="valS"
                     /locus_tag="PK710_03150"
     CDS             complement(4931..7609)
                     /gene="valS"
                     /locus_tag="PK710_03150"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOT08737.1"
                     /translation="MNQNKMFRTIDAASLPKHFDAAEAEKRWDATWEKNDVHRYDPDR
                     PRHETFAVDTPPPTVSGSLHIGHVFSYTHTDVIVRYQRMRGRNIFYPMGWDDNGLPTE
                     RRVQNYFNVRCDPHVPYEPGLTLTEATGKAAKDKPRIISRPNFIELCLQLTAEDEKAF
                     TNLWRRIGLSVDWKEQYATIDDHCRTLAQLSFLDLFEKQHIYNHEAPTMWDVDFQTAV
                     AQAEVEDRPTAGAFHDIVFGVEGSDDSLMISTTRPELLAACVGVTAHPDDARYRHLFG
                     KRAITPVFHVPVPIFPSELADPQKGTGILMVCTFGDATDVLWWREQKLALRQIVGRDG
                     RLMPVQFGVQGWESLHSDKANEAYAQLSGKSVKQAKAIMVELLRMPQCSAHGPECPLR
                     GEPKPLQHAVKFFEKGDRPLEFVTTRQWFVRLMDQKEALLAKGDEVQWHPEFMRSRYR
                     NWTENLQLDWCISRQRYFGVPFPVWYPLDEQGQPDYSKPIVAPRERLPVDPMSDTPPG
                     HDPSQRDQPGGFTGESDVFDTWFTSSLTPQIGTHWQLAPARHEKLFPMDIRPQSHEII
                     RTWAFYSIAKALLHENKVPWNHVVISGWILDPDRKKMSKSKGNVVTPMHLLDEYTADG
                     VRYWAANAKLGSDTAFDEKVLKVGRRLVTKLFNAGKFVLGQSASVHPIQCELDRAFVH
                     TLRQMCSKATESFDAFEYAHALMATESFFWTHFTDAHIELVKARARGECSCDGVDDEA
                     ARGSAVASLRLGLSVLLRMFAPVVPFITEEVWSWVFAEETGHPSIHRAPWPTEAEFAS
                     IAPPEDASSFDVAVACWTAINKRKSEAGVSVARPAEILVLAAHPHTVTVLERVVQDVM
                     AAAHCKNFRIEKDESLAEGMFDVLQAVFEEKKTDPS"
     gene            7760..9850
                     /locus_tag="PK710_03155"
     CDS             7760..9850
                     /locus_tag="PK710_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOT08738.1"
                     /translation="MGLTRSTSIVFLLLALCPSAAGAREAAQPMGPLDPGDAAWAKKQ
                     PKAPARSAAPKKNRSNKPQQPPAPAVRRATAGPGAEPPPSPEPPELQALRQAERELFG
                     TDDLLVPSDGCPPELLPDASNTLVFSTGLPPSSVAPRAQNTSPQPDYLSSLRMPDVPV
                     KFTSRVVQYLDFYKNDSRGRAVAAIFYRKSGRFEPMLRSVLRHHGLPEDLMWVAVVES
                     GLNPTIYSPVGAAGLWQFMPESGRLYGLVIDRWVDERLDPVRSTHAAAMYLQDLYRRF
                     GTWELALGSYNMGYGGMLAALRKYNTNDFWELSRFEAGVPWETALYVPKILALAVVGK
                     NPEVFGLANLTRDPPAEFDEVDVVPGVSLESVAKAAEVDVSDIGQLNPQLLASRIPPR
                     APTRAAAGVWTVRVPKGHGAKVEANLVQTRVAEPELVPYTVRHGESLADIAAAHGTTT
                     QELSRHNALTSDEPIRPGLLLMVPPRRRASPTTEKIAVAVPVDLQPPAGYQRRFYQAV
                     AGDTLQGVANALSVRVDDLIRWNGLDVRARLHPGMLLLTLLPATQTLSRVRLLSDDDV
                     QVFVVGSDAFFDYHEAQKGRVRMVTVVQSGETWRSIAARTGLSLGMLERINQRSRSTP
                     LQEGEKLVVYVPASKVAAQPKPAPQAKDPELSTPEPSESVSEDMPLVPPQDTQDSDTS
                     VSAEPSEPLEVDSK"
     gene            complement(10084..10959)
                     /locus_tag="PK710_03160"
     CDS             complement(10084..10959)
                     /locus_tag="PK710_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08739.1"
                     /translation="MGTNITPHAWLERIAKPLILGGPLYPFDPIGPSHAPSLAQQIAS
                     VASPTDISSMTVARVRHARHLYPVDTLPDISVEEWLMTIAIHDVLRATDPHLQSVFSP
                     GRAVNILDGALAILAQVPAPKHTLEALARHATFASVFAMQRQDIAVSWWCGSRLYAGR
                     KPPDRLLAWPEVRRVRSEILQQNLQSMMTGSETLKAHHADAWQALLVRTPLTDLMNVT
                     RPLPPFRWTPTTVAMLSGPGRDIAMRALRWQSDPQTYSTCYASFVRLGDSAPAIVKTA
                     LEELFAWNNPANQRT"
     gene            complement(10969..12414)
                     /locus_tag="PK710_03165"
     CDS             complement(10969..12414)
                     /locus_tag="PK710_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08740.1"
                     /translation="MLDRVAMHQSRLRLPGLGTDGKGVALGSRGLVLLASLERLVAFL
                     SLYTSCQSLADVLATLRIEVVKSKMGTREVVLSFAAEGSERMDRIAEMARVALGYTFT
                     GSSRHFVQYRDIQAPFGYDVPEVMAADGDYVLYHNSFSQVYHRERDLDLRGLLLRLHL
                     VQDPAFGRDPGPCLLVAEEGMGPAIVQYLIRSRVEARVGIAQWPPLSALDDAPVQRYL
                     FEVPALPPRMLPLFQQTPGVTALRVVSPGIAIQLGYRHPITLQSCPVFPPQGLVLFFG
                     DQHEPLVLDTMPTLGSVSAFARVEFHGGTGMQALSGQQLNVPTTKVPIRLLPSIEPWS
                     QVQATFIPTADYPTFRHMLYRLDTLTLKTASIAFTPHGAFLLNPKGIDTTPIGYFLRE
                     LRPRVFLAAGYDIVPAIDSDVLFQALGAPSNHHVFLLPQVPAIGVPNQAFVPLPKAMI
                     EGQSWAPVNTLPIEPALTMPIPQVVFDNPPR"
     gene            12546..13118
                     /locus_tag="PK710_03170"
     CDS             12546..13118
                     /locus_tag="PK710_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08741.1"
                     /translation="MGNGDGNGNYLHGLQVLVSGIRVALDDLVTAGHEAELVREEVRD
                     RIDLVIELLRALQALEAGSDGRVQVVDGRSLLWLVRCTHPEVFMGKMPMAVWVRTNVQ
                     AALELLDVVVSWVRAEGTRVRLVLVAHPPSLFVVGSPSDGYSPASPLDREMEQTIMSL
                     AGAARVEASLRDYPRTAKLDFLLAEEDFVE"
     gene            13216..14868
                     /locus_tag="PK710_03175"
     CDS             13216..14868
                     /locus_tag="PK710_03175"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOT08742.1"
                     /translation="MPRVPGRLLTMTASVFAAIGLSCPPAGWQQDTAAAGPAEPIAVA
                     EAPATIEEPLVVFDTPLSAEPLSEGQEPSVEEPSPSDLEAATGEIEVEPIDETENERE
                     LAEEPIPPPQQAFELVALRKETWIFAEPKWGSRRIGYLRAGMSVRRHEHPAGFKHCQQ
                     GWYPIEPQGFVCVGKSATLEKHHPMAEAAGHPPNRGLAMPYPVVMADYPPPPRYVRLP
                     TEAEQQEVEPDLAKHRQRGKDLSAWESLLGDVPGSLLYQRSLPSPHAKPRPQGILHTG
                     RPVPRTGFGLLQVFSWTDRVFGVTTDLDLIPLDRTKAVVRSTMVGTHLVEDQGLPLAF
                     VHAHGVRQYRIDLETRAVQDAGEVRFREGFSLTGRRVRAGENVYLETKEGTFLREGPR
                     MVIVPALQEVPSWAKAGRKWIDVSILRQTLVAYEGTRPVFATMVSTGADGLGDPKETH
                     STVLGAFLIHTKHVSATMSNDEPGDVYDLQDVPFVQFFHEGYALHGAYWHDGFGTPRS
                     HGCINLSPYDAAWLFGWTDPEVPEGWHGRLQLRGGTLVYTHP"
     gene            complement(15155..16192)
                     /locus_tag="PK710_03180"
     CDS             complement(15155..16192)
                     /locus_tag="PK710_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08743.1"
                     /translation="MASCNISIRFDRNPPVYSPGDTISGTIDVVANASCHCDALVPHL
                     CWKTGGKGDTDSEKYVHESLGTFDWNPGESYQYPFSFVAPAGPVSYQGSIVHVNWFVE
                     ARADVPWAIDPRASEPLMLLPLAAERKASAAYREQPHLSAKHHKLGNGPRSQSKWENR
                     VLTLILGSIFLVLAIAQQTIHGSSVPFLIPMVFVAGVMLKFAWNDLRNLVARRMVGAL
                     SVTIDPPTATRGETLRIQAAFTPNSNGRLRYAKATLYGEEIAWRGSGKSRTRYHQDLD
                     KIDAELCGSLAFFQNQPLELSCDLQVPEHAAPSFRSVNNRVQWSVLLHLDIKGWPDMK
                     ETIPIDLHPSS"
     gene            complement(16387..17424)
                     /locus_tag="PK710_03185"
     CDS             complement(16387..17424)
                     /locus_tag="PK710_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08744.1"
                     /translation="MSSCDISIRFDRNPPACSPGETISGTIDVVANASCRCDALTAHL
                     SWKTSGKGDTDSEKVDVVPLGTFEWKRGESYQYPFSFLAPTGPVSYQGLIVHVNWFVE
                     ARADVPWAIDPRASETLLLVPLPAERKASTAYREQPHLSAKHHKLGNGPRSQSKWENR
                     VLTLILGSIFLVLAIAQQTIHGSSVPFLIPMVFVAGVMLKFAWNDLRNLVARRMVGAL
                     SVTIDPPTATRGETLRIQAAFTPNSNGRLRYAKATLYGEEIAWRGSGKSRTRYHQDLD
                     RVEAQMCGSSAFFQNQPLELSCDLRVPEYAASSFRSFNNHVQWSVLLHLDIENWPDLK
                     ETIPIDLLPSL"
     gene            complement(17608..17862)
                     /locus_tag="PK710_03190"
     CDS             complement(17608..17862)
                     /locus_tag="PK710_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08745.1"
                     /translation="MRDTDDSTRHQHDLDKIEGQMCEALPFYQNQPQELSCELPVPNH
                     AAPSFDSSNNCVQWNLEIHLLIENSPDLKETIPITLLPSA"
     gene            complement(18040..18642)
                     /locus_tag="PK710_03195"
     CDS             complement(18040..18642)
                     /locus_tag="PK710_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08746.1"
                     /translation="MSTYDMSIVLDRSPPVYSPGERISGTIVVVANESCRCDDLAVDL
                     YWKTSGRGNSDTEQETLGAFEWQEGESYRYAFSFSAPPGPVSYQGTIIHVEWFVETRV
                     DMPWRKLSANACETITLQPSTVERDTSATQRKQPYPSAFPALSGNPPRDFSKPKIPVV
                     TIITGLMLLVTNIFLVITRAHIGLFLLLNALFLSVMSTLS"
     gene            complement(18684..>19320)
                     /locus_tag="PK710_03200"
     CDS             complement(18684..>19320)
                     /locus_tag="PK710_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOT08747.1"
                     /translation="CDCSRKEISLASAPSGNEKTPIYPGSCRQLTQSRSFRRPPATRL
                     RIPPNTFIEFEDAVFGKQSSQLSQDVGDFVLRRGDGVFAYQLAVVVDDLAQGITHVVR
                     GADLLSSTARQIFLMRCLGTEPPSYAHAPLWVGPNGEKLAKRASGIPVRDHRNAGVDP
                     RKLIARLAQALGLADQNEPWLTPHELIPRFSWGKITKGPIEIDPSRDSLPL"
BASE COUNT         4034 a         5208 c         5877 g         4201 t
ORIGIN      
        1 accgcatagc cacaatcctt gttgaacatc tgaattccca caagccccat ccccgaaggc
       61 aaagtcggtt tggattccac gaatgttgcg ccgtcttgcg tgcgcacgac cgtggaaccg
      121 cccaccgcga acatcgccca atcatgcgtg acttgtagat gaaggatttc ctccggcgcc
      181 tggtacaact cgtcccaacc catcccggcc gacgtcgtac gccagatcag gttttcttgt
      241 ccggtcatcg gcgccacgcg catcccggac atccacccgg tgttcgcgtc gaggaacgcc
      301 acctgcgtgg ggatcgtgta ctgcatcgcg ccccccgtcc ccgatggcaa agacatatat
      361 ccccaatcgg ttccgttgat ggtcctccat cccgcgggag tttggctact gccttgcttg
      421 gagattccca ccgcccaggc cgtgtttccg tcgatcgctg ataggctcat cacggcgttg
      481 ccccggttga cctcgctatg cacgagggac caggattcgt ttgctcttgc tccaggagcg
      541 aggagtccca cggccatgcc gctgacgaac attattaaaa tgttgaataa tcgtgatgtt
      601 acaagcatat cgcccgctcc cggaccgagg tttgccgaag gcttgtgccc agtttcccac
      661 ggagtgggac ttgcgccaaa cctttgccgc gccggaacgt ggttgttttt cgaaacagtg
      721 caatggcagg ggtttagctt gttggagcag gggtttgccc gtatcggaaa gcagccgcgc
      781 acgtgccttc cgaagatacc atgcacgccc cttgcggcga atcgggcgta cacacggtgc
      841 cgaaaagctt gcattcgaag ggggtgagaa tacctcgcag gatgtcgcca caggcgcaac
      901 cgcgtggctc tcgcgtgggt tccacctcta cctcgaaacg atccgcgtcg aagtctcgaa
      961 atgccgagcg gaggcgaagt cccgatgctg gcaaggaccc aaggccccgc catgtcgaat
     1021 ccacgggctc gaatacgcga tcgagaagcg ccaaggccac ccgattgcct tgtggttcta
     1081 ccgctcgacg atacgcattt tgcaccttgg cttgtctatc caccacttgc tcgagcagca
     1141 tcgagatgcc ctgaagcacg tccagggctt cgaatccggc aatcacgcat ggcaccccat
     1201 gatcgtttgc caacggctca tacacgctcg tgcccaagat ggcgctcaca tgaccgggac
     1261 agagcaaacc atcgagttgc aatcgttcat cttgaacgat gacgtccagg gcgggagcta
     1321 cccgcttggc tgccgatgca atgaaaaagt ttttaattcc gcgatgttgc gctgaaacca
     1381 ccgccgcggc gatcgtgggg gaagtcgttt cgaaccctac tccgagaaag acgaacgatc
     1441 ggttcggctc gcgttctgcc atgtcgacgg catccagggt ggagtacacg acgcggatgt
     1501 cggctccttt tgctcgctcc cgttgcagcg atgaagtgga tccgggcacg cgcatcatgt
     1561 ccccaaaggt tgtgatcacg aatccgggtt gtcgtgataa cgcaatggcg cgatccaggt
     1621 acgcgtgagg tgtgacacaa accgggcatc ccggtccgga cagcattttc acatgctttg
     1681 gcagcagcga ccggattcca tgacgataca gcgacatggt gtgggttccg cacacttcca
     1741 tgaacgttgc cttggccagt ggtttggctt gttcatgaat gcgcgcgacc agacctcgca
     1801 cgggggcggg atcgcgaaaa gcggagatga aggggatatt tttcatctgg gttccagcgg
     1861 ttcttccgaa aggagctgga gcatcgatag tgtttcttga gcctcctggg cgctgagttt
     1921 ctggatggca aagcccgcgt ggacgatcac atactcacca ggctgcacgt cgggaaccag
     1981 cgccgtgccg atttctcgct ccacgccatc gaccatcgcg atggctcggt catcggagac
     2041 gcgaagaact tgcatgggaa ccgccagaca catagcttga ccataggagc cgttggttct
     2101 ggcaacaagt ctaccgtttg ctgacaatcg atgccgactc gaacacctcg attctgctac
     2161 accatctcca tgaaaatgac gttgcgtctg gggatagcgt gcagctttgg atggctgctc
     2221 aacgcttgtc cttcggatca ggctcccctt ggtacgggag aattgggcaa gggggatttg
     2281 cggatcgtat gtgccgacga cgatgcggtt tgtgcgtcgg gtgccacgag tctcgatgcc
     2341 gcgtttccca ttgccgtggg tgcgagtatt caggtcgttt atgacggccc tgtgcctaca
     2401 tcgcccaccg gcgggccttc gacaatgacc attttctccg cgtcgcccgc gatgttatcc
     2461 aaagacgagg caagctttgt cgcgtggatg ccgggggtgg tggcgttgct cgcgcgaacc
     2521 caagaaggca cggtggcgga cttcgtccat ttgaccttgg caagcgtgca acgtctcgaa
     2581 ctggattctt ccccggaacc cctcgtggtg ggtggaaaaa aagtgtggaa agtcgcaccg
     2641 ttaggcgcgg atggcgctgt gttggcgggc agcctttcgt acacctggga ggtggaagga
     2701 accacggtgg agatcgtcga aagcgcgggc cgttcggttc aattgtccgg aaaacagccc
     2761 ggtacggcca tcttgcgagt caaaacaggg ggtgtggttg gggaagtcac gctcaccgtg
     2821 gaggcgccat gagccgcctg gtttggcttg tgccattgtg gtgcatcagc ttgctgggag
     2881 gctgcagctc cgatgagggg ttcgacctcg agcgaagcac ggacgcgccg ggtgaggttt
     2941 ccgttaccgc gtcgtcggtg acgcttcccc aaggggttgc ggtgggtgtc cgacttcgcc
     3001 ttcacaggtc tgaagacccg gaaaatggcg agtttacgac cgccatgcgg tcgaccaacc
     3061 cgcagatcgt ccaggtgtgg ccgacgacgg agcgcaacac gttcgtgctt tgtggggcat
     3121 cggaaggcgt tgcttccgtt accgttttgg tcgaggccca agatgatcga accattgccg
     3181 ttgtcgtgga tccaccgctg agggtgacgc catgagccgt cggcaacgcg ctcgttggct
     3241 ccttgggatt ctcctatttg cggttggatg tagcggcgag gacgaagggt cgagctggtc
     3301 gtgggggcct gccgatggtg gcgacgaagg cctggccgat caggtcgtag acaccgcgat
     3361 ggaggccgcg gatagcggtg agatggggga cgggcaaccg gacgatggga aggtcgaagc
     3421 gcacgaagac gtgcatggcg atgtcccgga cgaacaaccg gatgatgcgc aggaagaagc
     3481 gtcggaggat gccttcgacg cggggccatg cccggaggat atggtgttgg tcgaacagac
     3541 ctgcatcgat cgcttcgaag cgccgaatcg gcaaggagca tcgccgcttg tgatgttcca
     3601 ttttctggaa gccgaatcgt ggtgtgaaac gcgcgggaaa cgtttgtgtt tcgacgatga
     3661 atggacgcga gcttgcgggg ggccccaggg tcattcctat ccgtatgggg atagtcatgt
     3721 gtctggggag tgtacggatg acaaggcgtg gcgggcgtat gagcagcagc ttctcaacgg
     3781 atggccctgg tcgatcgcga ccgatgcaat cgaaagcctg gaggcgttgt tggaagcggt
     3841 aaggagcaag ggggcagggg caaaggcggc ggcagaccac gtggcttggc tataccaggc
     3901 cgaaccgagt ggttccaaac agggctgtac ggctggagag ggcacctatg accttgcggg
     3961 caatgtggaa gagtggacac gtcgacgcag tgggggcacg acgggatatc acggcaattt
     4021 gaaagggcga tattgggcgg agtcgcgcac ctgtcagcaa tcggtcacga cgcacggcga
     4081 cacgtttcgg ttttatgaaa tcggttttcg atgctgcaag gacgtggagt gacggggttg
     4141 ccgaatattt aaaataattc gtaaaaataa atagttacag ccgatccggg aaggtgtagg
     4201 gcatgggggt gaggggtgaa gcggccgaac aggttcaacc gctgccgtac atttcgtcgt
     4261 aatcctgatg tgcggcatcg atgatcgcgt gggcttcctt ggctccgagc acccgttcga
     4321 tctcgaccac gttggaggtt tctccgggaa tgagtttgta ggtgagaaag tagtgtcgaa
     4381 gtcgttcgac cagcacttgg gggacgtcgc ggatgtcttg cgccatgccc cagacgttgt
     4441 ctttttcgag cacggcgatg attttgtcgt cggcttcctc accatcgatc atttgtaacg
     4501 cgccgatgac ccgtgcattg acgaggattt cgcctcggga gatgggcctt tcgctcaata
     4561 cgcagatatc caaggggtct ccatctccgc gtttcgagtt gggcgacagg gcttgcacgc
     4621 gttgagcgca gagcgtccgc ggaatgaatc cataaagcgc ggggggttgg gaagagctgc
     4681 gctgaggacg gtcgacacgc agatagccgg tttccttgtc gacctcgaat ttgaccgtgt
     4741 caaagggggt gatttcgata aaagcgtgga ccgtgatcgg tggttggggt cctaacgtaa
     4801 gaccgtgcca aggatgcgga cgccagcggt aaaaaggttt gggaaaggac atgggcaatg
     4861 aatgctgcgc tatcggcggt gtggatggta tcgctgtggc tgtcgggggg aaaagtacga
     4921 gtgctttcgt tcagctcgga tcggtttttt tctcttcgaa gaccgcttgc agcacgtcaa
     4981 acataccttc cgcgagggat tcgtcttttt caatccgaaa attcttgcaa tgcgcggcag
     5041 ccatgacgtc ttgcacgacg cgttcgagca cggtgacggt gtggggatgc gctgcgagca
     5101 ccaggatttc cgccgggcgg gcgaccgaaa cgcctgcttc gctctttcgt ttgttgatcg
     5161 cggtccagca agccactgcg acgtcgaacg aggaagcatc ttcgggtggt gcaatcgagg
     5221 cgaattccgc ttcggtcggc cacggtgccc gatgaatgct cgggtgcccg gtttcttccg
     5281 cgaaaaccca cgaccatacc tcttcggtaa tgaagggaac caccggggca aacatgcgaa
     5341 gcagcacgct caatccaaga cgcaaggatg cgaccgccga gcctcgggca gcctcatcgt
     5401 caactccgtc gcacgagcat tcacctcgtg cccgcgcctt gaccaactcg atatgtgcat
     5461 ccgtgaagtg cgtccagaag aacgattccg tggccatcaa cgcgtgggca tattcgaaag
     5521 catcgaatga ttccgtcgcc ttcgaacaca tctgtcgcaa cgtatgcacg aaagcgcgat
     5581 ccaactcgca ttggatcgga tgcacggagg cgctttgccc aagcacgaac ttgcccgcgt
     5641 tgaatagctt ggtgaccagt cttcgaccga ctttcagcac cttttcatcg aatgccgtat
     5701 ccgagccgag cttggcattc gctgcccaat atcgcacgcc atcggccgtg tactcatcca
     5761 gaagatgcat gggcgtcacg acgttgccct tgcttttgga catttttttt cggtccggat
     5821 cgaggatcca tccggagatg accacatggt tccagggcac tttgttttcg tgaagcaagg
     5881 cttttgcgat cgaataaaag gcccacgttc gaatgatctc atggctttgc ggtcggatat
     5941 ccatcggaaa aagcttctcg tgccgcgcgg gggcgagctg ccaatgcgtt ccgatctgtg
     6001 gcgtgagcga gctggtgaac catgtgtcga aaacgtcgga ttcccctgta aatccgccgg
     6061 gttggtcccg ttgggaggga tcatgtccag ggggtgtatc ggacatcgga tcgacgggca
     6121 aacgctcacg gggtgcgaca atgggtttgg aataatccgg ctgaccctgt tcatcgaggg
     6181 gataccagac cgggaaagga acaccgaaat agcgctgacg actgatgcac cagtcgagct
     6241 gcaagttttc cgtccaattg cgatagcggc tgcgcatgaa ctccggatgc cactggacct
     6301 cgtctccttt ggcaagcagc gcttcttttt gatccatcaa gcgaacgaac cattgtcgtg
     6361 tggtcacgaa ctccagcggc cggtcgcctt tttcgaaaaa cttgaccgca tgctgcaacg
     6421 gtttgggctc gccccgtaac gggcactccg ggccatgagc actgcattgg ggcatgcgaa
     6481 gaagctcgac catgatggct ttggcctgct tgacgctttt gcccgagagc tgcgcgtacg
     6541 cttcgttggc cttgtcggag tgaaggctct cccagccctg cacgccaaat tgcacgggca
     6601 tgaggcgtcc atcacgccct acgatttgcc gcaacgccag tttctgttcg cgccaccaaa
     6661 gcacgtcggt ggcatcaccg aaggtgcaaa ccatgagaat gccggttcct ttttgaggat
     6721 ccgcgagttc gcttgggaaa atcggcacag gcacgtgaaa aaccggtgta atcgcgcgct
     6781 taccgaaaag atgccggtag cgtgcatcgt ccggatgggc ggtaaccccc acgcaagccg
     6841 cgagcaattc cggacgggtg gtggaaatca tcagagaatc gtccgaaccc tccaccccga
     6901 acacgatgtc atggaaagcc cccgccgtgg gcctatcttc cacctcggcc tgcgcgaccg
     6961 cggtttgaaa gtcgacatcc cacatcgtgg gcgcttcatg gttgtaaatg tgctgctttt
     7021 cgaaaagatc caaaaaacta agctgcgcca aggttcgaca atgatcgtcg atggtggcgt
     7081 attgctcctt ccaatccacc gaaaggccaa tccggcgcca tagattcgtg aaagcctttt
     7141 cgtcttccgc ggtgagttgc agacaaagct cgatgaaatt cgggcgtgaa atgatacgtg
     7201 gcttgtcttt ggccgctttt cccgtggctt cggtcagtgt caagccgggt tcgtaaggca
     7261 catggggatc acaacggacg ttgaagtagt tttgaacgcg ccgctcggtg ggaagaccgt
     7321 tgtcgtccca gcccatggga taaaaaatat tgcggccacg catgcgctga taccgaacga
     7381 tgacgtccgt atgcgtataa gaaaagacat gcccgatgtg caaagagccg gaaacggtgg
     7441 gtggtggcgt atcgacggca aacgtttcgt ggcgaggccg atcggggtca taacgatgga
     7501 cgtcgttttt ctcccaggtc gcatcccagc gtttttcggc ctcggcagcg tcgaaatgct
     7561 tgggcaaaga ggcggcatcg atggttcgaa acatcttgtt ttgattcatg ggactgaacc
     7621 ttccgtcggc ccggggtggg gcaggagccc cgtagatcaa actggcaacc acgtaaagag
     7681 gggcgtcgag gggttgttga ccctgatcac gagcaatttg acggaatcgt gggtacgtga
     7741 catgaccgtt tcttgattca tgggactcac tcgatccacc agcattgttt ttctcctgct
     7801 ggcgctttgt ccttcggcgg cgggcgcgcg ggaagctgcc caaccgatgg ggccgctgga
     7861 tccgggggat gcggcgtggg cgaaaaaaca gcccaaagcc ccagcccgat ccgcggcccc
     7921 caaaaaaaac cgctccaata aaccccaaca acctccagct cccgccgtgc gtcgagctac
     7981 cgctggtccc ggagccgaac ccccgccatc ccccgaacct ccggaactcc aagccctccg
     8041 gcaagccgaa agggagcttt ttggcaccga tgacctgctc gttccctccg acggctgtcc
     8101 ccccgaattg ctccccgatg ccagcaatac gctggtgttt tctacaggat tgcccccgtc
     8161 ttccgtcgct ccacgagccc aaaacaccag cccccagccc gattacttat cctccctgcg
     8221 tatgcccgat gtgcctgtga agttcacctc ccgggtcgtg caataccttg atttttataa
     8281 gaatgattct cggggaagag ctgtcgcagc catcttttat cgaaaaagcg gacgattcga
     8341 gcccatgctc cgttcggtgc tccgacacca cggattgcct gaagatctca tgtgggtcgc
     8401 ggtcgtggaa agcggactca accccacgat ctactccccc gtcggcgccg ccggcctctg
     8461 gcaattcatg ccagaatcag gaaggcttta cggacttgtc attgaccgct gggtagacga
     8521 acggctggat cccgtacgct cgacccatgc ggccgcgatg tatttgcagg acctatatcg
     8581 acgtttcggc acgtgggaac ttgctctcgg ctcctacaac atgggttatg gcggaatgct
     8641 ggccgccctg cgcaagtata ataccaatga tttctgggag ttatctcgtt tcgaggcggg
     8701 cgtcccctgg gagactgccc tttatgttcc caaaatcctg gctcttgcgg tcgtgggcaa
     8761 aaaccccgag gtcttcggtc tcgccaacct tacccgcgat cctccagccg aattcgatga
     8821 agtggatgtc gttcccggcg tctccctcga atcggtagcc aaggccgcgg aagtcgacgt
     8881 ttccgatatt ggacagctca atccgcaatt gctcgcttcg aggattcctc cccgagcgcc
     8941 cacgcgtgcc gcggctggcg tatggaccgt gcgtgttccc aaaggccacg gagcgaaggt
     9001 cgaagccaac ctggtccaaa cccgcgtggc cgaacccgaa ctcgtgccct ataccgtccg
     9061 tcacggggaa agcctcgcgg atattgccgc cgcccacgga acgaccacgc aggaactcag
     9121 tcggcacaac gccctgacca gtgatgaacc catccgtccc ggccttttgt tgatggttcc
     9181 tccccgccga agggccagtc ccaccacgga aaaaatcgcc gtggccgttc ccgtggatct
     9241 acaacccccc gcgggatacc aacgcaggtt ttatcaagct gtggccggag atacccttca
     9301 gggtgtcgcc aatgcgttat ctgtgagagt cgacgatctc attcgttgga acggcttgga
     9361 tgtcagggcg cgactgcatc ctgggatgct gcttctgact ctgcttccgg caacgcaaac
     9421 gctgtctcga gtccggttgc tatccgacga cgacgtccag gtgttcgtgg tggggagcga
     9481 tgcttttttc gattatcatg aggcgcaaaa aggccgtgtt cgcatggtta cggtggtcca
     9541 atcgggggag acatggcgct cgatcgctgc ccgtacgggg ttgagtctgg gcatgttgga
     9601 gcgaatcaac cagcgctccc gaagcacgcc gctccaagaa ggggaaaagc ttgtggttta
     9661 tgtccccgca tccaaggtag ccgcacaacc caagccagcg ccgcaagcta aggatccgga
     9721 gctttcgacg cctgaaccat cggaatccgt ctcggaagat atgcctttgg tgcctcccca
     9781 agacacgcaa gacagcgata cctctgtttc tgccgagcct tctgagcctt tggaagtcga
     9841 ttcgaagtag cgcttctcgc atccaaaggg gtcagctctg agtgaactcc aactctaaaa
     9901 aaattataaa tattcaatag ttacacaata tcagagggtc gatggaccct cgatgcacga
     9961 gttgtttttg ttgagggtgg ccatgacgac ttgtggttgt ctggcgaggc acaagctcag
    10021 gttatcttgg tggggatgct gagcgcggta ccctattgct ggattttact gtaatcgacc
    10081 ggatcaagtg cgctgattag cgggattgtt ccaggcgaaa agttcttcga gtgccgtttt
    10141 gacgatggca ggcgcggaat cacccaaacg gacgaacgag gcatagcatg tactataggt
    10201 ttggggatcg ctttgccagc gaagcgcacg catggcaata tcccttccgg gtccggagag
    10261 catggcgaca gtggtgggag tccatcgaaa cgggggcaag ggccgggtta cgttcatcaa
    10321 gtccgtgagg ggagtgcgta cgagcagggc ttgccaggca tcggcatggt gggctttcaa
    10381 tgtttcgctt ccggtcatca tcgattgcag gttctgctgc aagatttcgc ttcgaacccg
    10441 acggacttcc ggccaggcga gcaggcgatc cggtggtttt cggcctgcat acaggcgtga
    10501 gccgcaccac caggatacgg cgatatcctg gcgttgcatc gcgaacacgc ttgcgaaggt
    10561 ggcatgtcgg gcaagggctt cgagggtgtg ttttggggct ggcacttggg caaggatcgc
    10621 cagcgcacca tcgagaatat ttaccgcgcg tccgggggaa aacaccgatt gtaggtgcgg
    10681 gtccgtagca cgaagaacat cgtggatggc gatggtcatc agccattctt ccacggagat
    10741 atcgggtaag gtgtcgacgg gataaaggtg tcgtgcgtgt ctaacgcgcg ctacggtcat
    10801 cgacgatata tccgtggggg aagcgacgga ggcaatttgc tgagcgaggg atggtgcatg
    10861 ggagggtccg attgggtcga aaggatagag gggtccgccc aggatcaggg gtttggcgat
    10921 ccgttcgagc cacgcatgag gagtgatatt cgtacccatg attggcgcct agcgtggggg
    10981 attatcgaat acgacttggg gaatcggcat agtcagggcc ggttcgatgg gaagggtatt
    11041 cacgggagcc caagactgcc cttcgatcat ggcttttggc agcggcacga aggcctggtt
    11101 gggcacaccg atggccggga cctggggaag caaaaacacg tggtggttcg aaggggcgcc
    11161 gagggcttga aagagcacgt cggaatcgat ggcgggcacg atgtcatagc ctgccgcgag
    11221 gaaaacccgt ggacgcaatt cacgaaggaa ataaccgatg ggtgtggtgt cgatcccctt
    11281 tgggttgagt agaaaagcgc cgtgcggtgt aaaagcgatc gatgctgttt ttagagtgag
    11341 tgtatcgagc ctatagagca tatgccgaaa ggtaggatag tcggccgtgg ggatgaacgt
    11401 tgcttgaacc tggctccagg gttcgatgct ggggagcagt cgaatgggga ccttggtggt
    11461 gggcacattg agttgttgtc cggagagcgc ttgcattccg gttcccccat ggaactccac
    11521 tcgcgcgaaa gcagagacgg agccgagggt gggcatggtg tcgagcacca agggctcgtg
    11581 ttggtcgccg aaaaataaca ccaagccttg agggggaaat acagggcagg attgcaaggt
    11641 gatgggatgg cgatacccga gttgaatggc gatgccgggc gaaacgacgc gcaacgccgt
    11701 tacaccggga gtctgttgaa acagcggcaa catccgtggg ggaagggctg gcacctcgaa
    11761 taggtagcgc tgaaccggtg cgtcatccaa agccgacaag ggtggccact gggcaattcc
    11821 cacgcgagct tcgacacgag aacggatgag gtattgtacg atggcgggac ccatcccttc
    11881 ttcggccacc agcaagcacg gccccgggtc gcgtccgaac gctgggtctt gcacgaggtg
    11941 caagcgtaac aacaatcccc gcaagtcaag gtcccgttct cgatgataca cttgcgaaaa
    12001 cgagttgtga tacaagacat aatcgccgtc ggcagccatg acttcgggaa cgtcatagcc
    12061 aaaaggtgct tgaatatcgc ggtattgcac aaaatgacgg ctcgacccgg tgaaggtata
    12121 gccgagggcg acccgagcca tttcggcgat gcgatccatc ctttcgctgc cctctgccgc
    12181 aaacgaaagc acaacctctc gcgtgcccat tttcgatttc acgacctcga tgcgcaacgt
    12241 ggccagcacg tccgcgaggg attggcaaga ggtgtaaagc gataggaagg cgacaagccg
    12301 ctcgagggaa gcgagcaaaa cgagtccacg ggatcccaag gcaacgccct tgccatcggt
    12361 accgagtccg gggagtcgca ggcggctttg atgcatcgcg acacgatcga gcatggccgg
    12421 tagtgtaata cagctttttg ccggtggata ccgcgggaat tgcctaagcc tccgaacccg
    12481 ttgccttgct gccaattcat cggcggctgg cattggatgc cgtgttgtgg ttgcatgaag
    12541 gtgggatggg caacggcgat ggaaacggca attatcttca cggacttcag gtgttggtgt
    12601 ccgggattcg tgtggctctc gatgaccttg tgacggccgg tcacgaggca gaattggtac
    12661 gagaggaagt tcgtgatcgg atcgatctgg tgatcgaatt gctgcgtgcg ttgcaagccc
    12721 tcgaggcagg aagcgacggt cgggtgcaag tggtcgatgg gcgttcgctg ctatggctcg
    12781 tacgctgcac gcatcccgag gtgttcatgg gaaaaatgcc gatggccgtt tgggtacgaa
    12841 cgaacgtgca agcggcgttg gaattgctcg atgtggtggt gagttgggta cgagcggaag
    12901 gcacacgtgt gcgtctggtc ctcgtggcac acccgccatc gctatttgtc gtgggttcgc
    12961 cctccgacgg ttattcgccg gcaagtccat tggatcgcga aatggagcag acgatcatga
    13021 gtcttgcggg cgcggcgcgt gtggaagcca gcctgcgcga ctatccgcgt acggcgaaac
    13081 tggatttttt gttggcggag gaagatttcg tggagtgaat ccaatccgat cgtcgttgcc
    13141 gtagaggcga acagcgccga attgctccaa gacttggccc ttggaccgtg aatcaggtag
    13201 cgccgaacga gtatcatgcc tcgtgttccc ggtcgcttac tcacgatgac agcgtcggtt
    13261 tttgccgcta ttggcctgag ttgtcccccc gctggttggc aacaagatac ggctgcggca
    13321 gggcccgcgg aacccatcgc ggttgcagaa gcaccagcca cgatagagga gccccttgtc
    13381 gtattcgaca cgccgttgtc ggcggagcct ctgtccgaag gccaagaacc atccgtcgaa
    13441 gaaccttccc cttcggatct cgaggcggcc acgggtgaaa tcgaagtcga acccatcgac
    13501 gagaccgaaa acgaacgcga attggccgaa gaaccgattc cccccccaca gcaagccttc
    13561 gaactcgtgg ccctgcgaaa ggaaacctgg atttttgcgg agcccaagtg gggatcgcgc
    13621 cgcatcggat acctccgtgc gggcatgtcc gttcgtcgtc acgagcatcc cgctggcttc
    13681 aagcattgcc aacaagggtg gtatccaatc gagccccaag gcttcgtatg tgttggaaaa
    13741 tccgccacat tagagaagca ccacccgatg gccgaggccg cgggacaccc tccgaaccgc
    13801 ggattggcaa tgccctatcc cgtggtcatg gcggattatc cacctccccc acgctacgtt
    13861 cgtttgccca cggaagccga acaacaagag gtagaaccgg atctcgccaa acaccgccaa
    13921 cgaggcaagg acctttccgc atgggaaagc ttgctcggag atgttcccgg ttccctgctt
    13981 tatcaacgat ccttgccctc tcctcacgcc aaaccgcgtc cgcaaggcat cctgcacacc
    14041 ggacgtcccg tgccgcgcac cggctttggt ttgttacaag tcttttcctg gacagaccgg
    14101 gtgtttggcg tcacgacgga cctcgatctg attcccctcg accgcaccaa agctgttgtc
    14161 cgctcgacga tggtgggaac ccatctcgtc gaggatcaag gcttgcccct cgccttcgtg
    14221 catgcgcacg gcgttcggca gtatcggatc gatctcgaga cgcgggcggt gcaagacgcg
    14281 ggggaggttc gttttcggga aggcttttct ctgacggggc ggcgtgttcg cgcgggggaa
    14341 aacgtgtatc tcgagacgaa agagggaacg tttttgcggg aagggccccg tatggtgatc
    14401 gttccggccc tccaggaagt cccctcatgg gccaaagctg gccgaaaatg gatcgatgta
    14461 tccattcttc gtcagacgct cgtggcgtat gagggaacac gtccggtgtt tgcgacgatg
    14521 gtttccacgg gagccgatgg tcttggcgat ccgaaagaaa cgcattcgac cgtgcttggc
    14581 gcgtttttga ttcacaccaa acacgtatcg gcgaccatga gcaacgatga gcctggggat
    14641 gtgtatgacc ttcaggatgt gcctttcgtg cagtttttcc atgagggata tgcgcttcac
    14701 ggggcgtatt ggcacgacgg ttttgggacg ccgcgatcgc atgggtgcat caatttatcg
    14761 ccttatgatg cggcatggtt gttcggctgg acggatccgg aggtgccaga agggtggcac
    14821 ggtcggttgc agcttcgtgg gggaacgttg gtttatacgc atccttgact cataaaagcg
    14881 cgtataatcg ataggttacg agtcttttgg gcgaggtgag ggggatggtt tctttcaagg
    14941 gggagaccaa gcggtgataa cacggccccg acagggttac tccaccacgg tgcctttgct
    15001 tctaccacgt agaggcaggc attttcacgg caaagatgcc atgttgcgct cccatccagt
    15061 gccgattgca cctttgatga ggacgattgg atacgagtct atcccttgtt catgatagcg
    15121 tggtaccagt atttaaatat tgattatcaa tagcttacga gcttgggtgc aagtcgatgg
    15181 ggatggtttc cttcatgtcg ggccaaccct tgatgtccag atgaagcaag acactccact
    15241 gcacacgatt gttgaccgat cggaacgaag gggccgcgtg ctccggcact tgcaagtcgc
    15301 aggacagttc caggggttgg ttttgaaaaa atgccaatga tccacacagc tccgcatcga
    15361 tcttgtcgag atcctggtga tagcgagtac ggctttttcc gctcccgcgc caggcgattt
    15421 cctcaccgta caaggtggct ttggcatagc gaagccttcc gttcgagttt ggagtgaacg
    15481 cggcttgtat tcgcaacgtc tctccccgag tcgcggtggg aggatcgatc gtgacagata
    15541 gcgctcccac catccgtctt gccaccagat ttctcaagtc gttccaggcg aatttgagca
    15601 tgacaccggc gacgaagacc atcgggatca ggaaagggac ggaggatccg tggatggttt
    15661 gttgcgctat tgccaatacc aagaagatcg atccgaggat gagcgtcagg acacggtttt
    15721 cccatttcga ctgggatctg ggaccattgc cgagtttatg atgctttgcg gatagatggg
    15781 gttgttcgcg ataggctgcc gatgccttgc gttcggccgc cagaggcagc agcatgaggg
    15841 gttcgctcgc gcgtggatcg atggcccagg gcacatcggc gcgagcctcc acgaaccaat
    15901 tcacgtggac gatcgatccc tgatagctga cgggtcctgc aggcgcaacg aacgaaaaag
    15961 gatattgata ggattcaccg gggttccaat cgaacgtccc cagggattca tgaacgtatt
    16021 tttctgaatc ggtatcccct tttccgcctg ttttccaaca caagtggggg accaaggcat
    16081 cgcagtggca ggatgcgttt gcgacgacgt caatcgttcc cgagatcgtg tctcctggcg
    16141 aatacacggg tgggtttcgg tcgaagcgga tgcttatgtt acaggaagcc attttgtttt
    16201 ctggagcggg tggttggact cgacaaggct ttcgacaatg gcccgttcca ggcctgttcc
    16261 aatctaccac acacaagtgc gcccgttcgt gccgatgacg ccgtggatcc atgagcgcat
    16321 gagccagtgg ccgcgttggg atcatcgaag cgaggtactg gatataattc gtttataatc
    16381 aatatgttac aggcttggca acaaatcgat ggggatggtc tctttcagat ccggccagtt
    16441 ttcgatgtcc aggtgaagca agacactcca ctgcacatga ttgttgaagg aacggaacga
    16501 agaggctgcg tactccggca ctcgcaagtc gcaggacagt tccaggggtt ggttttgaaa
    16561 aaacgccgat gatccgcaca tctgcgcctc gaccctgtcg agatcctggt gatagcgagt
    16621 acggcttttt ccgctcccgc gccaggcgat ttcctcaccg tacaaggtgg ctttggcata
    16681 gcgaagcctt ccgttcgagt ttggagtgaa cgcggcttgt attcgcaacg tctctccccg
    16741 agtcgcggtg ggaggatcga tcgtgacaga tagcgctccc accatccgtc ttgccaccag
    16801 atttctcaag tcgttccagg cgaatttgag catgacaccg gcgacgaaga ccatcgggat
    16861 caggaaaggg acggaggatc cgtggatggt ttgttgcgct attgccaata ccaagaagat
    16921 cgatccgagg atgagcgtca ggacacggtt ttcccatttc gactgggatc tgggaccatt
    16981 gccgagttta tgatgctttg cggatagatg gggttgttcg cgataggctg tcgatgcctt
    17041 gcgttcggct ggcagaggca ccagcaggag ggtttcgctc gcgcgtggat cgatggccca
    17101 gggcacatcg gcgcgagcct ctacgaacca attcacgtgg acgatcagcc cctgatagct
    17161 gacgggtccg gtaggggcaa gaaacgaaaa aggatattga taagattcac cgcgtttcca
    17221 ttcgaacgtc cccaggggca cgacatcgac tttttctgaa tcggtatccc cttttccgct
    17281 tgttttccaa gacaagtggg cggtcaaggc atcgcatcgg caggatgcgt ttgcgacgac
    17341 gtcaatcgtt cccgagatcg tttctcccgg cgaacacgcg ggtgggtttc ggtcgaagcg
    17401 gatgcttatg tcacaggaag acattttgtt ttctgaagca agcggttgga cccaacaagg
    17461 cttacagcaa tgcccttttc caatctacca catacgggcg tgccgtttta tgccgatgac
    17521 gctacggatc catgagcgca tacgccactt tccgtgttcg ctttcccgat gcgaagggtt
    17581 ggatattacg tgtttattat caacaggtta cgcgcttggc agtagggtga tcggaatggt
    17641 ttccttcaga tccggcgagt tctcgatgag taggtgaatc tcgagattcc actgcacgca
    17701 gttgttggac gaatcgaagg aaggagccgc atggtttggc acgggcaact cgcaggatag
    17761 ctcctgcggt tggttttgat aaaagggcaa ggcttcgcac atctgccctt cgattttatc
    17821 gagatcgtgt tggtgtcgag tgctgtcgtc tgtgtccctc atcgtccagg cgatttcctc
    17881 accatgcaaa atggccttcg cggagcgaag tcgtccgttc gatttgggcg tgaaatggac
    17941 acaaacccgc aacgtatctc tccgggtcgc ggtggaagga tcgatcgtga tggacagatt
    18001 ccccaacctc tgttttgcga atcgattgcg cagctctttt caactgagtg tggacatgac
    18061 ggagaggaaa agcgcgttca gcagaaggaa gagcccaatg tgcgctcggg tgatcacaag
    18121 gaatatgttc gtcacaagca gcatcaatcc ggtgatgatc gtcacgacag gtattttcgg
    18181 ttttgagaaa tccctgggag gattgccgga tagagcaggg aacgccgatg gatagggttg
    18241 tttgcgctgg gtggccgatg tgtcacgttc gaccgtcgaa ggctgtagcg tgatggtttc
    18301 acacgcgttt gcactgagtt tccgccaggg catgtccacg cgggtttcca cgaaccactc
    18361 cacgtggatg atcgttccct gatagctgac gggccccgga ggcgccgaaa aggaaaaagc
    18421 gtaccgatag gattcaccct cctgccattc gaacgccccc agtgtttcct gttcggtatc
    18481 gctatttccc cttccacttg ttttccagta caaatcgacg gccaagtcat cgcatcggca
    18541 ggattcattg gccacgacga caatcgtccc ggatatcctc tcccctggcg aatacacggg
    18601 tgggcttcga tcgaggacga tgctcatgtc ataggtggac atcttcgact ccggaaagaa
    18661 gagggtggct tccaccagac agtctacaag gggagactat cacgtgacgg atcgatttcg
    18721 attgggcctt tggtgatttt tccccaagaa aagcgcggta tcaattcatg gggggtgagc
    18781 catggctcat tctggtctgc cagacccaac gcctgcgcaa gtcgcgcgat gagctttcga
    18841 ggatcgacgc ccgcgttacg atggtcccga acaggaattc cagacgcgcg tttggcgagt
    18901 ttttctccgt ttggaccgac ccatagcggc gcatgcgcgt acgatggcgg ttcggttccc
    18961 aaacagcgca tcaagaaaat ctgacgagca gttgaggaaa gaaggtccgc gccgcgcacc
    19021 acatgcgtga ttccctgcgc gagatcatcg accacgacgg caagctgata ggcaaatacc
    19081 ccatcgcccc gacgcaacac aaaatcaccg acgtcttggc ttaattgaga actctgtttt
    19141 ccgaaaacgg catcctcgaa ttcgatgaag gtattgggtg gtatgcgcaa tcgtgtggcc
    19201 ggggggcgtc ggaagcttct ggactgcgtg agttgacggc aactgcccgg atatattggt
    19261 gttttttcgt tcccggacgg tgcgctggcc agcgagatct ccttgcgcga acagtcgcag
//
LOCUS       DAOSOR010000033        19272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_2846_length_19272_cov_4.396472, whole genome shotgun sequence.
ACCESSION   DAOSOR010000033 DAOSOR010000000
VERSION     DAOSOR010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 19272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19272
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2846_length_19272_cov_4.396472"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1032
                     /gene="tilS"
                     /locus_tag="PK710_03205"
     CDS             139..1032
                     /gene="tilS"
                     /locus_tag="PK710_03205"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOT08748.1"
                     /translation="MSPRQSHPPTLIRHVQRALREEKLVERGMTILVGVSGGPDSMAL
                     LHVLSMLRSKVGFALTAHAIDHGLRPEATQEIALARDFCHQFDVAFGVTQLQVAPGGN
                     LQARARAQRLAALRQAARANGAERIALGHHADDRAETVLIRLLRGSGPAGLAVLPPRS
                     NDLIRPLLRARRSDVMAHLQRHHIPFAQDPSNDDLRFLRARIRGQLLPMLVSLSPSIV
                     QHLCNLADDMAALGLSSTSLARAQLRELGRAASRHRAGVRVSVPGGGVAMVDMGTGRI
                     VVQTPEEMKGTRKPRQRPKDA"
     gene            1166..3103
                     /gene="ftsH"
                     /locus_tag="PK710_03210"
     CDS             1166..3103
                     /gene="ftsH"
                     /locus_tag="PK710_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOT08749.1"
                     /translation="MKQSHKTLLLWILLILMFLAIWQFVSPDTPRAAQVPFSEFVALV
                     KAAPEDTHVESVTIKDREYTFTVYDPASKQRSKKVTLGPAPTDDVLKLLEERKVKLAF
                     EKDEGSPFWSSALITVLPMIFLLVMFYLFMRQLQAGGGKAMSFGKSKARMLSETQNKV
                     TFADVAGINEAKDELEEIIAFLRDPKKFQRLGGRIPKGVLMMGPPGTGKTLLARAIAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQGKKHAPCIIFIDEIDAVGRHRGAGLG
                     GGHDEREQTLNQLLVEMDGFESNEGVIIIAATNRPDVLDPAILRPGRFDRRITVPRPD
                     VVGREGILRVHTKKVPLAEDVELETIARGTPGFVGADLENLVNEAALLAARQDKEAVS
                     MADIEMAKDKVLMGTERRSMVMSEQERRIAAWHEAGHTLVGMFIEGNDEIHKVSIIPR
                     GAALGVTQFLPTEDRHMMSRQQALARIAMALGGRSAEEIVFGEITTGASNDIQRATQI
                     ARMMVCEVGMSEKLGPVSYGEREDSIFLGREFAQHRPDYSEKTAIDIDEEIKRIINEQ
                     HTRAKNVLTQYRDALDRLAQALLERETLDQDEIRAVIDGRTLPQRERVVIPTWAERAE
                     QSKDKKRGTSIFGPPKPATSN"
     gene            3110..3715
                     /locus_tag="PK710_03215"
     CDS             3110..3715
                     /locus_tag="PK710_03215"
                     /inference="COORDINATES: protein motif:HMM:NF017736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HOT08750.1"
                     /translation="MRFSSAIHSILLGTLLGPLTPFPIAIEPPTPVLIRSASRKIFAP
                     IASDATEPAQVIPTSATLVRLHDQQIIVLSDTEPSPERFANLLRDRVTGDSVIMAPEL
                     LELLRKVTADTHEARVEFISGYRSWKLNEMLRKKGRNVASHSQHSLGKAMDFRLEGLS
                     SKQLAEKIEKIGWRGGLAHYPSDTDRFVHADVGPHRRWRGR"
     gene            3712..4992
                     /locus_tag="PK710_03220"
     CDS             3712..4992
                     /locus_tag="PK710_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08751.1"
                     /translation="MMCDSHNRTCSGVILNMHAHVTILLLALSLFGCRESPVTNPVFS
                     TTPSLDPPAASAPVSSVQPEDSSPPVPPPAPFMSDSEFGALIGALSEPTGDFPSDNYV
                     SNETSYLHVAQDLHDDKLRGTAYVGVGPEQNYTYIALMQPAMAYIVDIRRQNMLQHLL
                     FRALMEDADCRSVFLARLTCRPHLFMDPTQSKTASFDTIADALAKTEPPSSSMQQALI
                     QESLDRTHRLMERLGMTLHASDSASLRKILVAFSKEGLGLAYSMKGSGRKYPSIRELL
                     AMTSDDGGSGSYLSDEASYLRVRDMVRDNRVVPIVGDFSGARALRGVAADMRQRGLTL
                     GVLYTSNVEQYLFEAGSFGQFVSNVESFPLDSSSRFLRVWFDQGRAHPQQRKGHRTTS
                     LLMPVQPFLERHHKRPFPTYWHVTTTVMGDGASP"
     gene            complement(5105..6799)
                     /locus_tag="PK710_03225"
     CDS             complement(5105..6799)
                     /locus_tag="PK710_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOT08752.1"
                     /translation="MFRAGGTVSSIRLIHALNTVFSHELPSSLDLSWPSHAVVLLRPC
                     VRPIALPVVLTRESQSFQLPLMLSFRRREIMCCGGLWALLACGSNPSDNSHRSATDAA
                     ANDSGQADSEGKDAATNDAGLPDSALTDTPAIDTEMPDTEGGNDVEQEPDAPPSPPGP
                     VEYLGYFASAFDGHGSGDYSVDTAQAGANLTFIRAGLSTLGPKLTVAKTANQKAVVLV
                     HDHLFDWTAGLSLRTDWKERWDAIASVVEDGHTDTVAAFYPIDEPYWNAHLNGVSNDT
                     MKGWLVDIVTAIRERFPSKAVGTILAIDTLKKNLGSSYVGMFDWVGFDCYGPWDDCWG
                     QSMSWYVDTLGTWLTDQQRMISVPEAFLWGTSGTDLSAQHTIVTRLNRWHAQILSDDR
                     TIAVVPFYWPSMDLNGDGLTDTGAKDLPWIKERMAQMAASLLHPEQSRVFPTDEIASS
                     SYSTSLPFAATDRNDNSSWNAGGYAPQWIAFDLGNTNPLQRIELLTIQDPSGNTVHVL
                     EGSFDGASWFPIGTLAGVTDGNQLLSWSGTASTRWVRVMTTESPSWVGWREVRFFK"
     gene            7201..8910
                     /locus_tag="PK710_03230"
     CDS             7201..8910
                     /locus_tag="PK710_03230"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOT08753.1"
                     /translation="MLPPNEFRAREERQFSKMDAGLHGKIYTIGMRVNRGRWHGGLAL
                     VALAVAACGDDGSSVDSAVQGEGGQDQFTEAGSGGGAGSGEWAGEGGSGAGASGSNAG
                     QGGVGGGQGGQSGTAGDGGNAQGSCTTRITYGSFWIHDENHPEDFDVTSGIVTWDGAC
                     VSDGANSYATLSNGWKARFQGKGTCVIALDYSECEGVATTCQTRVTYGPAWKSDPNHP
                     ASYDDVKGTLAWNGVCHVEGSSSYARLSNGWTPYFKGKNTCDLAFRYTQCGGLYTNPV
                     LDVSCPDPGVVFDGTQYVMACTGGSKNAFPLRSSPDLVHWKSKGHIFPAASKPKWAVT
                     NFWAPEIHPVANGYVAYFTAQHTNGARSIGAATAPSALGPFEDIGKPLLNHASKIIID
                     ANQYQDPNGKLYLVWKEVVGNGTPQRRVTIYGRELKANGVEFVGTRKELITMDKDWEK
                     PHVEGPWLIDHGNWYYLFYSANEFSTKNYCVGVARAKSPLGPYKKADQPILRSAGKWA
                     GPGHGSVVTTPKGETWHIYHSWYAGNVGSDPGRIVLLDRVFWRDGWPTMFAAPSAVSL
                     PPP"
     gene            9292..10704
                     /locus_tag="PK710_03235"
     CDS             9292..10704
                     /locus_tag="PK710_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08754.1"
                     /translation="MKTLCFPSNVFLGLFALGCLVVGCSDENSNENGGDHAEEKACDP
                     VVQAQTTVHDGTSIEGAEHWTIGTHIVPRTIAIRSGATLTIDGCAIVRMGPDAGFEIA
                     PDSDGSITTLGTATKPVMVMRDEAEKPWGRVLDRGSGQLSFSHTTLVGGGGAPGPIDT
                     VDYGGATLVVWGDSAQLPELLTADGLSIIDSSGIGIMLRYARLTPSSRNLIVKGSSAY
                     PVYASIRVAGSLPVDSTLQSNGVDAVLLQTMGPAIYDADAPISEDVVLNDLGLPYRVG
                     HSLGSILVGDGSADGPSASLTLEAGVRLEFNRQGSTPNQLAVRGRQAGNGSYEPQGAL
                     IVKGLPSSPVVFTSAESVPAPGDWMGIFFKDVVDPRTHIEYAEIRYAGGDSGVVGACP
                     ALPGSSQGDADCSVIFWLQAPPPAGCISHSLIADGLGCGLYRGWHEEDMDLRDSNTFE
                     RLTGCQQSGVRQGGTCTACE"
     gene            complement(10833..12266)
                     /gene="aspA"
                     /locus_tag="PK710_03240"
     CDS             complement(10833..12266)
                     /gene="aspA"
                     /locus_tag="PK710_03240"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOT08755.1"
                     /translation="MMQESSEHGATRIEHDSLGSREIPSHAYYGIQTQRAVENFPITG
                     VTIGTLPHLIRALALVKKAAARANMDLGILPKDIGQAICRACDDLIGDKYHDQFVVDV
                     IQGGAGTSTNMNANEVIANLALEYLGYEKGDYAHCHPNDHVNLSQSTNDAYPTAIRLA
                     LYTKIDELTVAMAELKTAFESKEVEFADIIKMGRTQLQDAVPMTLGQEFGTHAVTIGE
                     DIARMHEAKKLLREMNLGATAIGTGINAPEGYADIVRQHLNELGHLSFVTAYNLVEAT
                     QDTGVFVQLSGVLKRFAVKLSKSCNDLRLLSSGPRAGLNEINLPPMQPGSSIMPGKVN
                     PVIPEVVNQICFQVIGHDVTISMAAEAGQLELNVMEPIILFSLYRTIEMLTQACRTLA
                     RRCIIGITANEDICRRHVEMSIGLVTALVPVLGYDRASLIAHKALKTGKSVTAVALEE
                     GVISKEELEDLLRPENMLRQKRIAKAP"
     gene            12394..13017
                     /locus_tag="PK710_03245"
     CDS             12394..13017
                     /locus_tag="PK710_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08756.1"
                     /translation="MDREKAHATLFKVAFGAAIIGCTASTPPPAMPSSSADKTTTTEK
                     TSGISDGAPWIGGQTTTEIAAPSFAILDFLCRPEAIWHILPRVDSVESLGQDGNDTLM
                     RISHKLGIFRGGYVVRIRPSIENDGRASITVRVDERYDRDVDKASALFVLEPNGTNST
                     RLHYHVQAMLYPGLIRWLFSGKIQWALMIVPDRIRAHIERQYSPHPP"
     gene            complement(13046..13612)
                     /locus_tag="PK710_03250"
     CDS             complement(13046..13612)
                     /locus_tag="PK710_03250"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HOT08757.1"
                     /translation="MNQTILAGIVSIVFLGTSLGCGAHSAKEPDAAASVVVGAGDKSR
                     PDEEANHISEDDEERSDGAAPPKGTMWGDSPSRGVGMDGSTIAFTPDKQAIQRVIRGK
                     KNEMRACYEGQLKSNPTLEGKLVIRFVIAADGTVASVQEENTTLSDEVMKACVMNVIR
                     GLFFPDWKSGVISIVYPFVFRVESSDAE"
     gene            complement(13695..14894)
                     /locus_tag="PK710_03255"
     CDS             complement(13695..14894)
                     /locus_tag="PK710_03255"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOT08758.1"
                     /translation="MHRITVLGGGLVGSVMARDLARDDDIHVTVVDADHQRLQRIARE
                     DGARLSTLHADLRDAEQVRNAVRDADAVVGAVPGWLGYQVLEQVIEASKPFVDISFMP
                     QDPRPLDAAARDKGVVGLVDMGVAPGMSNILATRLALDMQKPRDLRIYVGGLPQVRRW
                     PFQYAAVFSPADVIEEYTRPARLIENGQVVTRPALSEIETLDFEGVGTLEAFNSDGLR
                     SLMWTLQVPNMKEKTLRYPGHGEMMRMLRDTGYFAKQSVRVGQVDVRPLDLTLKLFET
                     AWMMPVGEGDVTVMRIEVEGEHEGKSAYFAYDLHDRFDLQTQTTSMARTTGFPCALAT
                     RLLLRRQLPLEPGIHFPEAVARHPKTLDNLIEGLRQRGISFQRKTEKPAAATPTTTPR
                     KLPTIPC"
     gene            15155..16687
                     /locus_tag="PK710_03260"
     CDS             15155..16687
                     /locus_tag="PK710_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT08759.1"
                     /translation="MTYDFLKDLGIEDVNSGVAYGPFLDKPSGGELVSESPIDGKPIA
                     TVLKASSKDYETVMNKAVAAFKSWRMVPGPKRAEVVRAIGDELRKYKEPLGKLVTLEM
                     GKILPEGLGEVQEMIDMADFAVGQGRMLYGLTMQSERVKHRMMEQWHPLGAVGVISAF
                     NFPVAVYSWNALLAAVCGDVTVWKPSSNTPLTAVATHRIFNRVAERFGFGEGIFNLVI
                     GSGREVGEMMLKDPRVPLVSATGSTDVGRKVAVRVGERLGRTILELGGNNAVIVLDDA
                     DLDLVVPAVLFGAVGTAVQRCTSTRRVLLQQGISAEFTRRLLKAYEQVRIGNPLHEGT
                     LMGPLVSKGAVSDMQAGLAKIREQGGEVIYGGEVLSGSGFESGCYVKPCIVKAKRDMP
                     IVQDEIFAPILYLIEVKDVDEAIDVHNDVPQGLSSSMFTLNLRHAERFLSPEGSDCGI
                     ANINIGTSGAEIGGAFGGEKDTGGGRESGSDSWKQYMRRQTNTVNYSIELPLAQGIKF
                     GD"
     gene            16878..17054
                     /locus_tag="PK710_03265"
     CDS             16878..17054
                     /locus_tag="PK710_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08760.1"
                     /translation="MALDSDETNKESNDATVALLREIRDIQKESLDWQRRFLWILIPI
                     FAVLVVQATLLLFR"
     gene            complement(17061..18929)
                     /locus_tag="PK710_03270"
     CDS             complement(17061..18929)
                     /locus_tag="PK710_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT08761.1"
                     /translation="MMGSSSHDQKQEPSHVRPIAIDDKGRVHLRQASELSESAAVSAD
                     KKTQAPKRRRPSSPPKPARKVILMLRWARRISQGLFLTIFLVLLFETTFRGSFSADPQ
                     ATVRLRGPVEAFFLFDPFAALLTFLSTFTVYRGLLWSLVVIALTLVFGRVFCGWICPM
                     GTINHILGWLLPSRRGKGGKRIAANRTSPVRQRVKVYLLLASMAAAVAGSAIGGIFDP
                     FSLLVRSIGLSFMPAGQYIARQTHERLEQSGVRALSTMADGVSDALANVVWQPSQFFY
                     HGGWIIGVLFIAVLFMNRFIPRFWCRVLCPLGALLGFLSRYALFGMIKRHDQCTDCNL
                     CLLHCQGADSPQGGVKWRQDECHMCLNCESACPEDVIEFRFLPGRESTISKPDTVRRT
                     ALASTAAGLVAIPTLRATGLLSIPFDAKRIRPPGSIAEKEFLDRCIRCGECMKVCPNN
                     ALHPALGEAGLEGLWTPVLIPRIGYCEESCVLCGQVCPTGAIRRFTEQERRGQGQPPI
                     KIGTAFYDFGRCLPWAMQTPCIVCEEFCPTSPKAIWLEDVEDVKRESKHGDDGSPPAM
                     TTVKLQRPRVDPMLCIGCGACEKVCPVVDEPAIRVTSVGESRSNTNVILLRENPKG"
     gene            complement(18939..>19272)
                     /locus_tag="PK710_03275"
     CDS             complement(18939..>19272)
                     /locus_tag="PK710_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOT08762.1"
                     /translation="MKNWYGLIGGRRNRLHQDINTSIADLAAFIRPTLTIIDAVRVLV
                     RNGPQGGNIDDTREMNTVIATLDEIAGDTYACELIGENPERVGYLRLGQQRGIGTMNL
                     KQVQIREV"
BASE COUNT         4235 a         5499 c         5476 g         4062 t
ORIGIN      
        1 tcgttttcgc ttcacttgcg agtgttgcgc gtggttcgat ggggagaatt gttcgcatac
       61 ctatccgaac gaagaccata agaaaatcga tctcgaccaa gtcgatcacg tcgttttttg
      121 caaggagttc gaattagcgt gagcccgcgc caaagtcatc cacccacgct gattcggcat
      181 gtgcagcggg cccttcgaga agaaaagctc gtcgagcgag ggatgaccat cctggtggga
      241 gtctccggcg gtccggattc gatggcgctt ttgcatgttt tgtcgatgct gcgttcgaag
      301 gtcggcttcg cgttgacagc gcatgccatc gaccatggtc ttcgtccgga agcgacacag
      361 gagattgcgc tcgcaaggga tttttgccat cagttcgatg ttgcctttgg cgtcacgcag
      421 cttcaggtag cgccgggggg caaccttcaa gcaagagcga gggcgcaacg acttgccgct
      481 cttcgacaag cagcacgcgc caacggtgcg gagcgtatcg ccctggggca ccatgccgac
      541 gatcgagcgg aaaccgtatt gattcgattg ttgcgtggct ctggtccggc cggtcttgcg
      601 gtattgcctc cccgctccaa cgaccttatt cggccgttgc ttcgggctcg acgttcggat
      661 gtgatggctc accttcaacg gcatcacatt ccgttcgcac aggatccttc caacgacgac
      721 cttcgttttt tgcgcgcacg gattcgcgga caactccttc cgatgcttgt ctctctttcg
      781 ccctcgatcg tgcagcatct ttgcaacttg gcggatgata tggcggcgtt gggtttgtct
      841 tcgacatcat tggcccgtgc tcaattgcgg gaacttgggc gtgccgcatc gcggcatcgg
      901 gcgggagttc gtgtttccgt gcccggtggg ggggtcgcca tggtggatat gggcactgga
      961 cgcatcgtag tacagacacc agaagagatg aaaggcactc gaaaacctcg acagcgccca
     1021 aaggacgctt aagctccgaa gtcgacgcca gaccgaagag atggcaaact tcaaaccgaa
     1081 cgaatggaac gcttctttgg ccccatcccc atgggactga acgaagcctt tattgccctg
     1141 acgaagacgt cgggaggctg atgttgtgaa gcagtcgcat aaaacgcttt tgttgtggat
     1201 cttgctgatc ctgatgtttc tcgccatttg gcaattcgtg agcccggata caccccgagc
     1261 cgcccaggtg cccttcagtg agtttgtggc acttgtcaaa gccgcaccgg aggataccca
     1321 tgtcgaatcg gtgaccatca aggatcggga gtacaccttc accgtgtatg acccggcttc
     1381 gaagcaacga agcaagaaag tcacgctagg tcccgcaccg accgacgacg tattgaagct
     1441 tctcgaagag cggaaggtga aacttgcgtt cgagaaggat gaaggcagtc cgttttggtc
     1501 gagcgcgctg atcacggtcc ttcccatgat ctttttgttg gtgatgtttt acttgttcat
     1561 gcgtcaacta caggcgggcg ggggcaaagc catgagtttt ggcaagtcca aagcccgcat
     1621 gctcagtgaa acccagaaca aggtgacatt tgccgacgtg gccggcatca acgaagcgaa
     1681 agatgagctt gaagagatca tcgcgtttct cagggatccc aagaagtttc aacggctcgg
     1741 ggggcgcatt cccaaagggg tgttgatgat ggggccgccg ggaaccggaa agacattgct
     1801 tgctcgcgcg atcgcgggcg aagcgggagt gcccttcttc tcgatttcgg gatcggattt
     1861 cgtggagatg ttcgtgggtg tcggcgctag ccgtgttcgt gacctgttcg aacaaggtaa
     1921 aaagcatgcg ccatgcatca tcttcatcga cgaaatcgac gccgtgggca ggcatcgcgg
     1981 cgctggtctt ggcggtggcc acgatgaacg ggaacaaacc ctcaaccaac tgctggtcga
     2041 aatggatggc ttcgaatcca atgaaggagt catcatcatc gccgccacca accgtccaga
     2101 cgttctcgat cccgccatcc tgcgacccgg aagattcgac cgtcgcatca ccgtcccaag
     2161 accagacgtc gtgggtcgag aagggatatt gcgcgttcac acgaaaaaag tgcctcttgc
     2221 cgaagacgtg gaactcgaga ccatcgcgcg aggcacaccg ggattcgtcg gtgcggactt
     2281 ggaaaaccta gtcaatgaag ccgcgctact tgccgctcga caagacaaag aagccgtcag
     2341 catggccgat atcgagatgg cgaaagacaa agtgctgatg ggcaccgaac gtcgctcgat
     2401 ggtgatgagc gagcaagaac gaaggatcgc ggcttggcat gaagcgggcc ataccttggt
     2461 gggcatgttt atcgaaggca acgacgagat ccacaaggta tcgatcattc cgcggggagc
     2521 agcgcttgga gtcactcaat ttcttcccac cgaggaccgg cacatgatga gtcggcagca
     2581 agcgttggca cgcattgcga tggcgctggg cggacgaagc gccgaggaaa tcgtgtttgg
     2641 agaaattacc accggagctt ccaatgacat tcagcgcgcc acgcaaatcg ctcgtatgat
     2701 ggtttgcgag gtgggcatga gcgagaaact cggccccgtt agttatggcg aacgcgagga
     2761 ttccattttc ttggggcgtg aattcgcgca gcatcgcccc gattattccg agaaaaccgc
     2821 gatcgacatc gatgaagaga tcaaacgcat catcaacgaa cagcatacgc gcgccaagaa
     2881 cgtgctcacc caataccgcg atgcgctcga ccgtctagca caggctcttc tcgagcgaga
     2941 aacactggat caggatgaaa tccgagcggt tatcgatggg cgcacattac cgcaacgcga
     3001 gcgcgtcgtc attcccacct gggcggagcg cgcggagcaa tccaaggata aaaaacgggg
     3061 gaccagcatc tttggtcccc ccaagcctgc cacttccaat tgacaggacg tgcgtttttc
     3121 ttctgcgatt cactcgattt tgctgggaac cctgctcggt cctctcacac cttttcccat
     3181 cgcgatcgaa ccgcctacgc ccgtcctcat ccgaagtgcg tccagaaaaa tatttgctcc
     3241 catcgcatcc gatgcgaccg agccagcgca ggtcatccct acctcggcaa ccctcgtgcg
     3301 cttgcatgat cagcaaatca ttgtcttatc cgatacagag cccagtcccg aacgcttcgc
     3361 aaacttgctt cgcgaccgtg tcacgggaga cagcgtcatc atggcgccgg agctactcga
     3421 attgctgcgc aaagtgaccg cggataccca cgaagctcgc gtcgaattca tttccggtta
     3481 tcgaagctgg aaactcaatg aaatgcttcg aaaaaagggc cgaaatgtcg cttctcacag
     3541 ccagcacagc ctgggcaaag ccatggactt tcgactcgaa gggctgtcct ccaaacagct
     3601 cgcggaaaaa atcgaaaaaa ttggatggcg tggcggtctg gctcattacc cttccgatac
     3661 ggatcgattc gttcatgccg atgtcggccc tcatcgtcgc tggcgaggac gatgatgtgc
     3721 gactcgcata accgtacctg ctccggcgtt atcctcaaca tgcatgctca cgtaacgatt
     3781 ctcttgctcg cgctttccct attcggatgt cgcgaaagcc cggtcaccaa tcccgtcttt
     3841 tccaccacgc cttcgctcga cccgccggct gcttcggcgc ctgtgtcaag cgttcaaccg
     3901 gaggactctt cgccaccggt tcctccgcca gccccattca tgtccgacag cgagttcggg
     3961 gctttgatcg gagcgctttc agaaccaacc ggcgacttcc ccagtgacaa ttacgtttcg
     4021 aacgaaacgt cttacttgca cgtcgcacag gacctccatg acgacaagct gcgaggtacc
     4081 gcctacgtgg gtgtgggtcc cgaacaaaac tacacttaca tcgccctgat gcagccagcg
     4141 atggcatata tcgtcgacat ccgacgccaa aacatgctgc agcatttgct gtttcgagcg
     4201 ttgatggaag acgccgattg ccggtcggta tttttagcgc gtctgacctg ccgcccacac
     4261 cttttcatgg atccgacgca aagcaaaacg gcaagcttcg acaccatcgc ggatgcgctc
     4321 gcgaaaaccg aacctccttc atcgtcgatg cagcaagcgc tgatacaaga atctctcgat
     4381 cgcacgcatc gtctcatgga acggctgggt atgacgctgc acgcaagtga cagcgcaagc
     4441 ctccgaaaga tacttgtggc gttttcgaaa gaagggctcg gccttgctta ttcgatgaag
     4501 ggaagtggaa ggaagtatcc atcgatccgt gagcttttgg cgatgacgag cgatgacggc
     4561 ggcagcggta gttatctatc ggatgaggct agttatttac gcgttcgtga catggttcgc
     4621 gacaatcgcg tggtgccgat cgtgggcgac ttttccggag cacgcgcgtt gcggggcgtg
     4681 gccgcggata tgcgccaacg aggcctgacg ttgggggtgc tttacacatc gaatgtggag
     4741 caatatttgt tcgaggcggg cagtttcggc caattcgtga gcaacgtgga gtcttttccg
     4801 ttggattcgt cgagtcggtt tcttcgcgtt tggttcgatc aaggtcgtgc gcatcctcag
     4861 caacgcaagg ggcatcgcac cacgagcctg ctcatgccgg tgcagccatt tctcgagcgc
     4921 catcataagc gcccctttcc cacctattgg catgtcacga cgaccgtcat gggcgatggc
     4981 gcatcgcctt gagcatctgc cggattgaaa aacgattcgg cgtcatgaca tcatcgtcgg
     5041 tcgcgcgaaa gaggtggaaa aggcatcgaa aagcgcgaaa taccgcgcga gcccaggagg
     5101 gggtctactt gaaaaatcgg acttcacgcc agcctaccca cgagggactc tcagtggtca
     5161 tcaccctcac ccaccgggtc gatgccgttc cgctccacga tagaagctgg ttgccgtcgg
     5221 taaccccggc gagtgtgcct atggggaacc aggaagcacc atcgaatgaa ccttcgagca
     5281 cgtgaaccgt atttccactc gggtcttgaa tcgtaagcaa ttcgatgcgt tgcagaggat
     5341 tcgtgttgcc gagatcgaaa gcaatccact gcggggcgta tccacccgca ttccacgacg
     5401 aattgtcgtt gcgatcggtg gcggcaaaag gcagggacgt ggaataggaa gacgaagcga
     5461 tctcgtcggt gggaaacacg cgtgactgtt cgggatgcag caaagacgcc gccatctggg
     5521 ccatgcgctc cttgatccac ggcagatcct tggcacccgt atcggtgagg ccatcgccgt
     5581 tcagatccat cgaaggccaa tagaacggaa cgacggcgat ggtgcgatcg tcggaaagga
     5641 tttgcgcatg ccagcgattc aagcgtgtga cgatcgtatg ctgcgcggag aggtccgtgc
     5701 cggaggttcc ccacagaaat gcctcgggca cgcttatcat gcgctgctga tccgtcagcc
     5761 atgtgccgag ggtatccaca taccaactca tgctttgtcc ccaacaatcg tcccaaggac
     5821 cgtagcaatc gaagcccacc caatcgaaca tgccgacgta cgagctgccg agattttttt
     5881 tcagagtgtc gatcgcaagg atcgttccta cggctttgct gggaaagcgt tcacggatag
     5941 cggtcacgat gtcgaccagc caacctttca tggtgtcatt ggatacgccg ttgaggtggg
     6001 cgttccagta cggctcatca atagggtaaa aagcggctac cgtatcggtg tgaccgtcct
     6061 ccacgactga ggcgatggca tcccagcgtt ctttccaatc ggttcgcagc gacaggccag
     6121 cggtccaatc gaagaggtgg tcgtggacga gaaccactgc tttctgattc gctgttttgg
     6181 cgactgtcaa tttgggaccg agggtggata gtcccgccct aatgaacgtg agattggctc
     6241 ccgcttgcgc ggtatctaca ctataatcac cgctgccatg gccgtcgaag gcactggcga
     6301 agtatccgag gtattcaacg ggacccggag ggcttggggg ggcatccggc tcttgttcga
     6361 catcgttacc gccttcggta tcgggcattt cagtatcgat ggcaggcgta tcggtcaatg
     6421 cggaatcggg cagcccggca tcgttcgtcg cggcatcctt cccctcggaa tctgcttgtc
     6481 cagaatcgtt tgcggcggca tcggtcgcgc tacgatggct gttatccgag gggttagaac
     6541 cgcaggcgag cagggcccac agcccaccgc aacacatgat ttcgcgacgc cggaaggaga
     6601 gcatgagggg cagttgaaac gattggcttt cgcgagtcaa gacgaccggt agtgcgatgg
     6661 gacgaacgca aggtcgaagc agcacgacag catgactcgg ccacgaaagg tcgaggctcg
     6721 agggcaattc gtgagaaaag acggtgttca gcgcgtggat gaggcgaatc gaggatacgg
     6781 tccctcctgc ccgaaacacg cgaaacaata tgtattctat atcaatggga catgcaagcc
     6841 ttcaaagatg ggactgccga ctgcgcaaag gaaacgccat ccttcttgga aagggcaacg
     6901 cctgtctgcg agaggggatg gcgataagca tttaaaatta atccttcatc gacttcacga
     6961 tgcaagaaca ggttgctgag agcggaattt attttttatc gtatgagatt cctccaagcc
     7021 tgtcatgggc gtggctggcg ggcgctggca cttttttgcc gcattcccgc aagggtcgag
     7081 tcgtgggcaa gtccggaggt aatctgcgat tgacagttca tggtccgtct tgccgtcagt
     7141 ccccagcggt gagcggacac gacattcagt gatatctatt tgatttaacg gatacatttc
     7201 atgctaccgc cgaatgagtt ccgagcgcgg gaagagcgcc agttttcgaa aatggatgcc
     7261 gggctgcatg gaaagattta cactattggc atgcgtgtga atcgagggag atggcatggg
     7321 gggcttgcgc ttgttgcctt ggccgtggcg gcttgtggcg acgatggctc ttccgtggac
     7381 agtgcagtcc agggcgaggg cggacaggac cagttcacgg aagccggctc aggcggcggt
     7441 gcgggttcgg gagaatgggc aggcgaaggc ggttctgggg caggtgcaag tgggtccaac
     7501 gccggacagg gcggcgtggg tggagggcaa ggagggcaat ccggtactgc aggggatggc
     7561 ggcaatgccc aagggtcttg cacgacccgc attacgtacg gttctttctg gatccatgac
     7621 gagaatcatc ccgaggattt tgatgtgact tcaggcatcg tgacctggga tggagcatgc
     7681 gtttccgacg gggccaattc gtacgccacg ctatccaacg gatggaaagc ccgtttccag
     7741 ggaaaaggaa cctgtgtcat cgccctggat tattccgaat gcgagggtgt tgccaccact
     7801 tgtcaaaccc gcgtgaccta cggtcccgcc tggaaatcag atccgaatca ccctgctagc
     7861 tacgatgatg tgaaaggaac gctggcttgg aatggagtat gtcacgtcga gggctcttcg
     7921 tcgtacgcaa gactatccaa cggctggact ccctacttca aagggaaaaa tacttgtgat
     7981 ctggcttttc gttacacgca atgtggaggg ctatacacca atcccgtact ggatgtgagt
     8041 tgtccggatc ccggggtggt attcgatggc acccaatacg tcatggcatg cacgggagga
     8101 agcaaaaatg cctttccgct tcgaagttcc ccagacctgg tgcattggaa atccaaaggg
     8161 catattttcc cggccgcctc caaacccaag tgggctgtca ccaacttttg ggcgccagag
     8221 attcacccgg tcgccaacgg gtatgtcgct tattttacgg cacagcatac caatggcgcc
     8281 cggagcatcg gtgccgcgac tgccccttct gccctcggac ccttcgaaga catcggcaaa
     8341 cctctgctga accacgcctc caagatcatc attgatgcaa accaatatca ggacccgaac
     8401 ggaaagctgt acttggtctg gaaagaagtc gtcggaaacg gaacacccca acgacgagta
     8461 acgatttacg gtcgagaact gaaagccaac ggcgtcgaat tcgtcggcac ccggaaagaa
     8521 ctgatcacca tggacaaaga ctgggaaaaa cctcatgtcg aagggccctg gctgatcgac
     8581 catggtaatt ggtattatct attttatagt gccaacgaat tcagcacgaa aaactattgt
     8641 gtgggggtgg cgcgagcgaa atctcccctt ggtccgtaca aaaaagcgga tcaaccgatt
     8701 ctccgcagcg cgggcaagtg ggctggacca gggcacggaa gtgtagtgac cactcccaaa
     8761 ggagaaacct ggcatattta ccattcatgg tatgcgggca acgtgggcag tgatccaggt
     8821 cgcatcgttc ttctcgaccg agtcttctgg cgcgatggct ggcccacgat gttcgctgcc
     8881 ccctccgccg tgtcgttgcc tccaccatga tgcccctccg ccttacacgt ctttcccccg
     8941 tgcaacgtac tggctctcag gtacaactgc cccttgccca tccgtccttt ggctccacaa
     9001 cgtcactcat cacgtaggta cgtggacgga ctggcaagca ctccgcgatg gtgtgagcgt
     9061 ctcgcggttg attccagcca cgacaagcgt tggccgatcc ctcctcgaga tgcgaccttg
     9121 gtcgatgagc aggaacgcct gcctgcaagg cgctgcgcat tctcccatga cgacgcacga
     9181 caataccgtt cgtggtgtgc tcttcgaacc gatgctgtct tgtcaaatca gcggtgcgcg
     9241 ccagataggg cagtggacac ccagctcttt gttttccagg tgaggtgctt gatgaaaacg
     9301 ttatgcttcc cttcgaatgt gtttctgggt cttttcgccc ttggctgcct cgtggtgggt
     9361 tgcagtgatg aaaacagcaa cgaaaacggt ggcgaccatg ccgaggagaa agcctgcgac
     9421 cctgtcgtcc aggcgcaaac cactgtccat gatggcacct cgatagaagg cgcagagcac
     9481 tggaccatcg gtacccacat cgtgccaagg acgatcgcca tccgatcagg ggcaacacta
     9541 accatcgacg gctgcgccat cgtgcgcatg ggacctgacg cgggttttga aatcgcgccc
     9601 gacagcgatg gaagcatcac cacgctgggt acggctacca agcctgtcat ggtgatgcgt
     9661 gatgaggccg aaaaaccgtg gggtcgagtg ctcgaccgcg gctcagggca actatctttc
     9721 agccacacca ccctcgtcgg tggcggcggt gctcccggac ccatcgatac ggtagattac
     9781 ggcggagcca cgctcgtcgt ctggggcgat agcgcccaat taccggaatt gctcacagcg
     9841 gatggactca gcatcatcga ttcgtcaggc atcggaatca tgctgcgcta tgcgcgtttg
     9901 acgccctcgt ctcgcaatct aatcgtcaaa gggtcgagcg cataccccgt gtatgccagc
     9961 atccgtgtgg cgggaagcct tcccgtcgac agtacgctac agagcaacgg agtcgatgcc
    10021 gtattgcttc agaccatggg cccagcgatc tacgacgccg atgctcccat cagcgaggat
    10081 gtggtgctca acgaccttgg tttgccctat cgcgtcggcc atagccttgg ttcgatcttg
    10141 gtgggagatg gcagcgccga tggtcccagc gcctcgttga cgctggaggc gggcgtccga
    10201 ttggagttca accggcaggg tagcacgcca aaccagttgg cggtaagggg ccggcaagca
    10261 ggaaatggaa gctacgagcc gcaaggcgcc ctgatcgtta aaggcctccc ttctagccca
    10321 gtcgttttta cctccgccga atccgttccg gcgcccggcg attggatggg aatcttcttc
    10381 aaggacgtgg tcgatccgcg gacgcatatc gaatacgcgg agattcgtta tgccggcggc
    10441 gatagcgggg tggtcggcgc atgccccgcg ctacctggtt cctcgcaggg tgatgccgat
    10501 tgctcggtca tcttctggtt gcaggcgcca ccaccggcag gatgcatcag ccattccctc
    10561 atcgctgacg gcctgggttg tggcctgtac cggggttggc atgaagagga tatggatttg
    10621 cgcgactcca ataccttcga acggctcacc ggctgtcaac aatccggagt ccgccagggc
    10681 ggcacctgca cggcgtgtga gtaaccccac gccgccgcgc atggttttca caccttcatg
    10741 ggaggttttt tcccaccagg agaacggtca caacgagtat caagagcgat gattcgatac
    10801 gttgagcagc ggatggttcg acagcgttgg agtcacggag ccttggcgat tcgtttctgc
    10861 cgcaacatat tctccggacg caacagatct tcgagttctt ctttcgaaat gacgccttcc
    10921 tcgagggcca cggctgtcac gcttttccct gttttcaacg ctttatgagc aatgagggag
    10981 gctcgatcat agccaaggac gggaacgagc gcggtcacca atccgatact catttccacg
    11041 tgccgacggc atatatcctc gttcgcggtg atgccgatga tgcatcgacg agcgagggtg
    11101 cggcatgcct gggtaagcat ctcgatggtt cgataaaggg aaaacaggat gatgggctcc
    11161 atgacattga gttcgagttg ccctgcttcg gcagccatcg agatggtgac gtcgtgacca
    11221 atgacctgga aacagatctg gttgaccact tcgggaatga ccggattgac ctttcccggc
    11281 atgatggatg atcccggttg catgggaggg agattgattt cgttgagtcc cgcacgaggt
    11341 ccggacgaca gcaacctcaa atcattgcaa gacttggata gcttgacggc gaatcgcttg
    11401 aggaccccgc tgagctgtac gaacactccc gtatcttgtg tggcttccac caagttgtag
    11461 gccgtgacga aagacagatg ccccaactcg ttgaggtgtt gtcggacgat atccgcgtat
    11521 ccttcgggag cgttgatgcc cgtaccgatg gccgttgcgc caaggttcat ctctcgcagc
    11581 agcttcttgg cctcgtgcat gcgagcgata tcctcgccga tggtgacggc atgggtgccg
    11641 aactcttgcc ccaacgtcat ggggacagcg tcttgaagct gtgttctgcc catcttgatg
    11701 atgtccgcga actccacttc tttcgattcg aacgcggtct tcaactcggc catcgccacc
    11761 gtcaactcgt cgatcttcgt gtacaacgcc aagcgaatcg ctgtcggata cgcatcgttg
    11821 gtggattggg aaaggttgac gtgatcgttg ggatggcagt gcgcatagtc gcctttttca
    11881 tagccaaggt actcgagcgc gagattcgca atcacctcgt tggcgttcat attcgtcgag
    11941 gtccccgcgc ctccttggat gacatcgacg acgaattgat cgtggtactt atcaccgatc
    12001 agatcatcgc aggcgcgaca gatggcctgt ccgatgtcct tggggagaat ccccagatcc
    12061 atattggccc gagcagcagc tttcttgacg agcgccaggg cgcgaatcag gtgaggcaac
    12121 gtgccgatgg tcacacccgt gatgggaaag ttctccacgg cacgttgggt ttgaattcca
    12181 taatacgcat gggaaggaat ctctcgtgac cctaggctat cgtgttcgat tcgcgttgcc
    12241 ccatgctcgg aagactcttg catcatatct gctctccgaa aaaaaaacgc cgatgtggca
    12301 ttcggaccag gaaagccgac actgcccaac gccccgcatt cggtcaaggg tgggtcgact
    12361 cccatcgaga ggcatgacag agtgcagtca cggatggatc gcgagaaggc tcatgcaaca
    12421 ctattcaagg tcgcttttgg cgcagcgatc atcggatgca ccgcgtccac tcctccgcca
    12481 gccatgccat cctcatccgc cgacaaaacg acaactactg aaaaaacatc aggaatttct
    12541 gatggtgcgc cttggattgg cggacagacc accaccgaaa tcgccgcccc ttctttcgcc
    12601 atcctcgatt tcctttgccg cccagaagcc atctggcaca tcctgcctcg cgtcgattcc
    12661 gtggaatccc taggccagga cggcaacgac acgctgatgc gcatttcgca taagctcgga
    12721 atctttcgag gaggttatgt ggtgcggatc cgtccctcga tcgaaaatga tgggcgcgct
    12781 tcgatcaccg ttcgggtaga cgaacgttat gaccgcgatg tcgacaaagc ttcggccttg
    12841 tttgtgttgg agccgaatgg aacgaattcc acacgcctgc attaccacgt ccaggccatg
    12901 ctctacccag gcctgattcg atggctgttt tcggggaaaa tccaatgggc cttgatgatc
    12961 gttcctgatc gaatccgcgc gcatatcgag cgacaatatt cccctcaccc gccctgacca
    13021 cagccgaaac gtcaggttgt ccccttcatt ccgcgtcact tgactcgaca cgaaacacga
    13081 atggatagac gatcgagatc acaccgcttt tccagtccgg gaaaaacaga cctcgaatca
    13141 cgttcatgac acaagccttc atgacctcat cggataaggt ggtattctcc tcctgcacgg
    13201 atgccacggt gccgtccgcg gcgattacaa accgtatcac cagcttgccc tccaaggtag
    13261 gattgctctt cagttgccct tcgtagcacg cccgcatctc attttttttg cctcgaatca
    13321 cacgctgaat cgcttgtttg tcgggcgtaa acgcgatggt cgaaccgtcc atccccaccc
    13381 cccgcgatgg ggaatcaccc cacatcgtcc ctttcggagg tgctgctccg tcgcttcgtt
    13441 cctcgtcatc ctcggaaatg tgattcgcct cctcgtcggg ccgggactta tcccccgccc
    13501 cgacaaccac gctggcggca gcgtcgggtt ctttggccga atgcgcccca catcccaagc
    13561 ttgtccccaa aaagacaatc gatacgatac ccgcaagaat cgtctgattc atgtacgagc
    13621 cgtagcacgc ccaccgcttc gcgggcacgg tgaatggtga cgctcgaaac tgcaagccca
    13681 tcgtggcaag caggctaaca gggaatcgtg ggaagctttc gcggagtcgt ggtcggcgtt
    13741 gcagccgcgg gtttttctgt ttttcgctga aacgagatcc ctcgctgtcg aaggccctcg
    13801 atcagattgt ccagcgtttt gggatgacgc gcgaccgctt cggggaagtg aattcccggt
    13861 tcgagaggaa gctggcggcg aagcagcaaa cgagtcgcaa gcgcgcacgg aaaaccggtc
    13921 gttcgtgcca tcgaggtcgt ctgcgtttgc agatcgaacc gatcgtgcaa gtcataggcg
    13981 aaataagccg actttccttc gtgttcgcct tccacctcga tgcgcatcac ggtcacatcg
    14041 ccttctccca ctggcatcat ccaggccgtc tcgaatagtt tcaaggtaag gtccagggga
    14101 cgcacatcga cctgtcctac gcgcacggat tgtttcgcga aatagcccgt gtcgcgaagc
    14161 atccgcatca tctccccatg acccgggtag cgaagcgttt tctctttcat attcggtact
    14221 tgcagtgtcc acatcagcga ccgcaaccca tcgctattga aggcttccaa ggtgcccacg
    14281 ccttcgaagt ccagagtctc gatctcggac aacgccggac gggtcaccac ctggccgttt
    14341 tcgatcagac gagcgggtcg tgtatattcc tcgatgacat ccgcgggcga aaacacggcg
    14401 gcatattgga aaggccaacg acgcacttgt ggcaaaccgc cgacatagat cctcagatcc
    14461 cggggttttt gcatatcgag agcgaggcgc gtggcgagga tattgctcat cccaggggca
    14521 acccccatat cgaccaaccc aaccacgccc ttgtctcgcg ccgcggcatc gagaggacgc
    14581 gggtcctgcg gcatgaaaga aatatccaca aacggcttcg atgcctcgat gacctgttcc
    14641 aggacttggt aaccaagcca gccggggact gctcctacca cagcgtcagc gtcgcgcacc
    14701 gcattgcgca cttgctccgc atcacgaaga tccgcgtgaa gcgtggacaa ccgcgcccca
    14761 tcttcccgag cgatccgctg aagccgctgg tgatcggcat cgacgaccgt aacgtgaata
    14821 tcatcgtcac gcgcgagatc tcgagccatg acggagccaa cgagtcctcc accgaggacg
    14881 gtaattcggt gcatgaaaac ctccaaaggc cgatacggta ctggtaacgg atcgacacga
    14941 gcaaggggga gatgggaaca accgtgcaga ttcgtgtccc ttggcgaata aagctctcgg
    15001 aaagcccctt ggctcgaaaa gggaggcgag attgccttga ctcccgcatc gtcgctgcgt
    15061 actcgggcaa agcggactca cagatcccca gcgtagcttc gatcatgggt tgccatggcg
    15121 cgcagctatc cagcattgct ccaaggagtg ttcgatgacg tacgatttcc tcaaagacct
    15181 cgggattgaa gatgtgaact ctggagtcgc ctacgggcca ttcctggaca agccaagcgg
    15241 cggagaactc gtcagtgagt caccaatcga tggcaagccc atcgccacgg tgttgaaagc
    15301 atcctccaaa gactacgaga cggtgatgaa caaggcggtc gcggcgttca agtcgtggcg
    15361 catggtacct ggccccaagc gtgcggaagt cgttcgagcc attggcgacg aacttcgaaa
    15421 gtacaaagaa ccgcttggaa aactcgtcac cctcgagatg ggaaaaattc ttcccgaagg
    15481 gctcggcgaa gtgcaggaaa tgatcgatat ggcggacttc gccgttggcc aaggacgcat
    15541 gctttacggg ctgacgatgc aaagtgagcg cgtcaaacac cgcatgatgg agcaatggca
    15601 tcctctcggc gcggttggcg tgatcagtgc cttcaacttc ccggtcgcgg tgtattcatg
    15661 gaatgccctg cttgccgcgg tctgcggcga cgtcaccgta tggaagcctt cttccaatac
    15721 gccgttgaca gcggtggcga cgcatcgaat tttcaatcgc gtggcagagc gtttcggctt
    15781 tggcgaaggg attttcaacc ttgtcatcgg aagcggtcgc gaagtggggg aaatgatgct
    15841 caaggacccg cgggttccgc tggtcagtgc taccggcagc accgatgtgg gacgcaaggt
    15901 agcggttcgg gtgggcgagc ggctcgggcg gacgattctc gaactcggtg gcaacaacgc
    15961 ggtgatcgtt cttgacgatg ctgatttgga tctcgtggtc cctgccgttc tgtttggcgc
    16021 cgtgggcacc gccgtacaac gatgcacctc gacacgccgc gtacttcttc aacaaggcat
    16081 cagcgcggag ttcacccgac gattgctcaa ggcttacgag caggtgcgaa tcggcaatcc
    16141 cttgcatgag ggaactttga tgggcccgct cgtgagcaag ggagccgttt cggacatgca
    16201 agccgggctc gccaagatcc gcgaacaagg cggagaggtc atctacggcg gcgaggttct
    16261 ttccggctcc ggcttcgaaa gcggctgcta cgtcaaaccg tgtatcgtga aggccaagcg
    16321 cgatatgccc atcgtccaag acgaaatctt cgcgcccatc ctgtatctca tcgaggtcaa
    16381 ggacgtggac gaagccatcg acgtgcataa cgacgttccg caaggcttgt ctagttcgat
    16441 gttcactctc aatctccgac atgccgaacg attcctttcc cccgaaggat cggattgcgg
    16501 catcgccaac atcaacatcg gcacatcggg cgccgagatc ggtggcgctt tcggcggcga
    16561 aaaggatacc ggcggtggac gcgaatccgg aagcgattcc tggaaacaat atatgcgacg
    16621 ccagaccaac accgtgaatt actccatcga attgccgctc gcccaaggca tcaagttcgg
    16681 cgactgatcc ctgctcgcgc ctgcttccag accctagttc ttcacgatca tcctttcgcg
    16741 ccatgaacgg ttctgcctct gaatcgcgcc aggtttccgc ttgaagccat cgcgctggtc
    16801 acggatgacg aaaagccgca cactcttccc tctcggcacc cgtatccgaa tagaatgaaa
    16861 ccaaaggagg ctgccccatg gcattggaca gcgacgaaac gaataaggaa tcgaacgacg
    16921 ctaccgtggc gttacttcgg gaaatacgcg atattcaaaa ggaatccctg gattggcagc
    16981 gacgtttcct atggatactg atcccaattt tcgcggtgtt ggtcgtgcaa gccacgctgc
    17041 tgctcttccg atgagcggca ttatcccttt gggttttcgc gcaagaggat gacgttggtg
    17101 ttcgagcgcg actctcccac actcgtcaca cgaatcgcgg gctcatcgac caccggacat
    17161 actttttcgc atgcgccaca tccaatgcac aacatcggat cgacacgagg acgctgaagt
    17221 ttcaccgtgg tcatggccgg cgggctgcca tcatcgccat gcttgctctc gcgttttacg
    17281 tcttcgacat cctcgagcca aatcgctttc ggcgaggtag ggcagaactc ctcgcatacg
    17341 atacaaggcg tctgcatggc ccacggaagg cagcgtccga agtcataaaa cgccgttccg
    17401 attttgatgg ggggttgtcc ctgtccgcgc ctttcttgct cggtaaaacg tcgaatggcg
    17461 ccggtgggac atacctgtcc gcacaacacg caggactcct cgcaatatcc gatgcgtgga
    17521 atgagcacgg gagtccacaa gccttcgaga ccagcctcgc ccagggcggg atgtaacgcg
    17581 ttgttgggac agactttcat gcattcgcca caacggatgc atcgatcgag aaattccttt
    17641 tccgcgatgg atcccggcgg gcgaatccgt ttggcatcga aagggatgct caacaacccc
    17701 gtggcacgaa gcgtgggaat cgcaacgaga cccgcggccg tggatgccaa cgccgtacgt
    17761 cgcacggtat ccggtttcga aatcgtgctc tcacgccctg gcaaaaagcg aaactcaatc
    17821 acatcttcgg gacatgcgct ctcgcaattc aaacacatgt ggcattcgtc ctgacgccac
    17881 ttcactcccc cttgcggaga gtctgcgccc tggcaatgca gcaggcataa attgcaatcg
    17941 gtgcattgat cgtgacgctt gatcatgccg aacagcgcgt agcgagatag aaatcccaac
    18001 aaggcgccga gcggacagag cacgcggcac cagaaccggg gtatgaatcg gttcatgaac
    18061 agaacagcga tgaacagaac cccgatgatc catccgccgt gatagaaaaa ctgcgaaggt
    18121 tgccacacca cgttcgccag ggcatcgctc accccatcgg ccatggtgga tagagcgcga
    18181 acaccagact gctccaggcg ctcgtgcgtt tgccgagcga tgtactgtcc cgccggcatg
    18241 aacgaaagtc cgatcgatcg aaccaggagc gaaaacggat cgaagatgcc gccgatcgcg
    18301 cttccggcca ccgccgcggc catcgatgcc agcagcagat acaccttcac gcgctgacga
    18361 accggagacg tacgattggc tgcaatgcgt ttgccccctt tgcctctgcg cgaggggagc
    18421 aaccacccaa ggatgtgatt gatcgtgccc atggggcaaa tccatccaca aaacacgcgg
    18481 ccaaatacga gcgtgagcgc tatcacgaca agcgaccaca gcaatcctcg gtacacggta
    18541 aaggtcgaaa gaaacgtcaa gagcgcggcg aatggatcga ataaaaagaa cgcttcgaca
    18601 ggaccgcgca accgtacggt cgcctggggg tccgcggaaa aacttccgcg aaaggtcgtt
    18661 tcgaacaaca gcaccaagaa aatcgtgaga aacaacccct gactgattcg ccgcgcccag
    18721 cgcaacataa ggatgacttt tcgagccggc ttcggagggg aagagggacg tcgtcgtttg
    18781 ggcgcctgcg tctttttgtc cgccgaaaca gcagcgctct ccgagagttc agaagcctgt
    18841 cgcaagtgta cccgcccctt atcgtcgatc gcgatcggtc gcacatggga aggctcttgc
    18901 ttctgatcat gggaagagga ccccatcatg gcgctccctt acacttctcg gatctgaact
    18961 tgcttgaggt tcatcgtacc gatacccctt tgctgcccca gccgaagata gccgacccgc
    19021 tcaggatttt ctccaattaa ttcacacgca tacgtatctc ccgcaatctc gtccagcgtt
    19081 gcgatgaccg tgttcatctc tcgtgtatcg tcgatgttgc cgccttgcgg cccattgcga
    19141 acgagaacac gaaccgcatc gatgatggtc aacgtcgggc ggataaaagc agccagatca
    19201 gcaatggaag tgttgatgtc ttgatggagt cgattgcgtc ggcctccgat gagtccgtac
    19261 caatttttca tg
//
LOCUS       DAOSOR010000034        18770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_2956_length_18770_cov_4.362009, whole genome shotgun sequence.
ACCESSION   DAOSOR010000034 DAOSOR010000000
VERSION     DAOSOR010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 18770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18770
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2956_length_18770_cov_4.362009"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(474..1988)
                     /locus_tag="PK710_03280"
     CDS             complement(474..1988)
                     /locus_tag="PK710_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08763.1"
                     /translation="MATRTSLRRLTSSVSLVALASFVGCTQGQPVEPEILGQSQEAIV
                     DVAHTPVERQSIGNCWLYAAATWVESMNLSYLEAQPATGGEHTCEHLFCEEGEKLTNG
                     CMPTESDHAACLAKICEEDSYCCETAWDSICVKHVTEERKCAASLCNAPPMGPAPDLE
                     PLDISQSYWTYWHWFDQIGGYMSDNEVSTGGSTWTSNAIMRDRGLMKGKDFVYADTQN
                     EMSSRQSAALSRVNAALKNGELSTSEARRNGALVREVLDKAWELTDDVRAQLDQVFGK
                     DGSQNLRSGASTAGTNIIDANSLPVRYAKRVNGKIEYKDTNLTEAIREWQNVRYPSSS
                     SGQREALIRMQRALHARQPVGITWNVDFNALENRHNERRGSFNMQTLKDMGRPGSQGG
                     HMTVLEDYEAETEEYGVLAAGVTLDPNNPQDAAKLEAALLPSTKIKLLRTKNSWGGAR
                     PDRAFAPGFPGYHDLWMDYLNGPFKWCPDEKNPTNENCRGETQGLREFLMPPGF"
     gene            complement(2015..2920)
                     /locus_tag="PK710_03285"
     CDS             complement(2015..2920)
                     /locus_tag="PK710_03285"
                     /inference="COORDINATES: protein motif:HMM:NF019057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HOT08764.1"
                     /translation="MMSRWTRNSGWSGGGWGRTVLLAPVALVLALGMFACESSSDEDK
                     SSGGQTDPTEGPGGKADNPNEAAFGGLMMQDMPEQPGRFDVSRTPAGDWFFRLLGPGG
                     DAFLTSGAYPQKTASLNAQLSVEENGVFLERYKVAPIGPDECSFELRAANNLVIAFGP
                     VFRDCAEADTRIQATRDLVAGVLQHKASVSKGAHFELWKESSDAKWRFELRAQDDRVL
                     LASQTYTSRSNAVTGIESVRANGKLPERYRIVAQQDGTVSISLKAGNGQEIAQSGPYP
                     AQDQAQAVIDESVALLVSERVGNPW"
     gene            complement(3361..4554)
                     /locus_tag="PK710_03290"
     CDS             complement(3361..4554)
                     /locus_tag="PK710_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08765.1"
                     /translation="MRKILLFLVSSGLMACGNSEDGGSGKTGSDAGGLFDTSPGVEAS
                     DTATLPDVTEVDGAAGVSAICEAYIACTAKTTPAGLGTVIATYGEKGTCWATGQAEMC
                     ESACKVGVVTVHKAFPGVEECNLCSESADCPPSIRACDSQAGRCVTCLSDSDCPSSDA
                     PACDISTHTCVACLQDHHCDGMTPVCNAAARRCVRCLTDDHCPADAHRCDTSTNQCLE
                     CIEDKDCAGSSKGAICLPMVRRCGCGLLGKGCAPSQLCEESVCCTPDCGTAVCGPSHT
                     YGCGSDNPYACGVCPNKGTCNQGACSDLGVECDPKASACYAGERCSFSVKTKDYRCLR
                     DAEGKDCNNIYACHVLAGDEGAYVCTNSRCRPHCLTASDCTSGHCEEWGVPISPSSPG
                     VCTLP"
     gene            4722..5858
                     /locus_tag="PK710_03295"
     CDS             4722..5858
                     /locus_tag="PK710_03295"
                     /inference="COORDINATES: protein motif:HMM:NF014027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HOT08766.1"
                     /translation="MHDPKESLPGLQRKLSHAEKDDGLEPVIPLDLGSIGSFDDLARA
                     MSRTAFGGRQLGEAVDVLVRMARDPECAVVLTLSGAMTVAKMGLVICEMIDKGLVQAI
                     VSTGALMAHGFVEATGLAHYKYREGMNDAELYEKGYNRVYDTLEPESNLEHVEHILGE
                     VFEQWDPSITLSSRRLNAQLGKYLVQHHPTVRGVLKSAVEKAVPVFVPAFSDSEIGLS
                     LGIFNRRQALLGKPRLSFDPFLDLEYFSSAVAVVPRMGIFTIGGGVPRNWAQQVGPYL
                     ELLERHLGQDGQGGIVRYQYGVRICPEPAHWGGLSGCTYSEGVSWGKFVPHERGGRFA
                     EVMADATIAWPLVVKAALERLDKLGGAVPKPVLSGHDIWKPAKS"
     gene            5920..7089
                     /locus_tag="PK710_03300"
     CDS             5920..7089
                     /locus_tag="PK710_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOT08767.1"
                     /translation="MNRTWPGRTAPWIRPPIAEITRQAAHYRTQGKDLIDLGQAILGL
                     APAENAKQRVRDYLEIPGPQSYSPDPGLPELRQAIAHLLIHRKAVSAACAERVMVTCG
                     ASQAFANALFTVTNPGDEIITFEPGYFDHNYAIAMASCRQVPVALRLRENSYDFDLEA
                     VVSALTPHTRAIVLVSPANPTGAVACPSFVERLCDLCRSRDIWLLSDETYDLFTFSPY
                     STTSPAAVDPTGKVMVLGSFSKTLGLAGWRIGYLYGPEPAIEEAYKVQDSLVVCASVA
                     SQHAALGALECLDSYVSFARQELTKRKDTLVSVLRAVSSLRPRMPRGATFLLATLEDQ
                     QDDIAFCHRLLAEAGVIAVPGSAFGPHGKGTIRLSFGNLPCESIQKAGERIKKMG"
     gene            7283..7951
                     /locus_tag="PK710_03305"
     CDS             7283..7951
                     /locus_tag="PK710_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08768.1"
                     /translation="MATKLSEVRQLCTKREIQLFKSVRPTSIPSYSPAALKKKIDRVR
                     TAQEKYEQLSRPQRRKARAAASSDSDPHAQRKAEIFGELLERLEARSALLEARKNKPK
                     KAASAKKTVKKAAPKKAAKKSVAAKKTTKKRTTKKAVKATASKASTIKSLEKPKKKAA
                     SPITAKKLAAPKTTKKRTITPKGQGKAVKKATKVQQRTQSKAVRGHIKAQGKRAQARR
                     DARR"
     gene            complement(8229..9287)
                     /locus_tag="PK710_03310"
     CDS             complement(8229..9287)
                     /locus_tag="PK710_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08769.1"
                     /translation="MSQLALQFRLAGIPVRVEPGFWLIALLLGMSGSAKTIVLWMAVV
                     FLSVLIHELGHALMARAFGASPEVTLYMMGGLTRSVYPSGHIHSRFRSALVTLAGPFA
                     GFVLAGLTFVLLLLVQPREGTPALTVGLMLLWINLGWGMVNLLPVLPLDGGNLLREVL
                     SGPGPEVGWVRALWVSVIVGPLVALASWKADMTWAAVLFAFFSYSAGKQLVQLSGIRK
                     DFGRGLDARLEQAQQALVEGQFEKALSLASEVAEQARTKELREHAIHLAVMAQLELGE
                     AQQALDRLERLSPDRADPFLYGLCLLSVDRPQEAVASLQRAVETKAHPKAKHVLVEAL
                     QRAGEQAAADELRKQLEI"
     gene            complement(9322..10890)
                     /locus_tag="PK710_03315"
     CDS             complement(9322..10890)
                     /locus_tag="PK710_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene dehydrogenase"
                     /protein_id="HOT08770.1"
                     /translation="MSEKHYDVIIIGRSLGAMALAALLARRDFSVMVIGQGARPADYV
                     FRDRVLRRRCFMPLAATSPAFKRILSELAQSQVFRRRLVALDPMLSVMLPDRRFELPP
                     DLEWLQREIDREFPEVRRVIDDLYHDLTRINGEADTAFEKDVCWPPETFWERRETNAA
                     ASSLPFVREDAHALLGGFPANHPYVDIVVQSAQIASNLMAGSRPLPSFAVGRLHGAWS
                     RGPFGLAAGEDDMISFFQQRVTALGGQVLLSDRVVSINPNGKDSHALVLDGGTAVLGA
                     TFVVTDGTGESLAHLAQGKGILRKAQRDWPMVSERTGRFTVSLLVRREGLPDALGAES
                     LVFPRMPGAPPDPQLPVVHLQRCDRMAEQPMPDDDGTVLLVAEVLLPMPGVLAVSETR
                     AVIVSILENHLPFLTPHLLIVDSTHDGLPVWVYDNGKRTTVDRIEARGTARVAEPMSP
                     VLSVEPLGYLGLAGEPLRGPLARSFLTGPSVLPPLGQEGELLAAWGVAKIITGSDNRR
                     VRMRRDMWSRIEFG"
     gene            complement(10887..11552)
                     /gene="purN"
                     /locus_tag="PK710_03320"
     CDS             complement(10887..11552)
                     /gene="purN"
                     /locus_tag="PK710_03320"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOT08771.1"
                     /translation="MEPLRLGVLVSGSGSNLQAILDAIRKKRLDATVCLVVSNQPKAL
                     AIERARRAGVATAIVNHKDYDSREAFDRALVTKMREAKVQWVVLAGFMRLLTPTFLEA
                     FPHRVLNIHPALLPSFPGINAQQQALDYGVRVSGCTVHLVDSGTDTGPILAQSVVDVR
                     ADDTVDTLSARILAREHVLFPKVLQWIAQGRVHIMASNGSSGRVLVQLDGVRASVGVA
                     GAR"
     gene            complement(11556..12599)
                     /gene="purM"
                     /locus_tag="PK710_03325"
     CDS             complement(11556..12599)
                     /gene="purM"
                     /locus_tag="PK710_03325"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOT08772.1"
                     /translation="MPISYKDAGVDIDAGDRLVERIKPMAQSVRVPEVLDGIGGFAGL
                     CSIPAGYKNPVLVSGTDGVGTKLEVAIRLGIHDTIGIDLVAMCVNDIATVGARPLFFL
                     DYFACGQLDVDVGEAVIRGIVEGCRQAGCALLGGETAEMPGMYAPGVYDLAGFAVGVV
                     ERDNIMDGRSMQAGDAVIGVASSGIHSNGLSLARRLVERALDGDYHRKINELDASLGS
                     VLLTPTRIYAKSILALASALGSSLRGVCHVTGGGIVGNLPRIIPEGLGAQLDAGAYER
                     PALFRWLAEVGPVNESEMYRTFNMGVGLCVVVSSDREKEALGVLRGEGETAFSFGRIV
                     PRHGAESARVCIR"
     gene            complement(12655..14130)
                     /locus_tag="PK710_03330"
     CDS             complement(12655..14130)
                     /locus_tag="PK710_03330"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT08773.1"
                     /translation="MPDITILNLNMLFVRYFDRIERERHLPLGTLYVTAALEAAGFEV
                     QHLDYQMHDAQDPFSSKEILAFVGEPAPVVGFSCMANLLPFTLLAMKEFKQAWPDRTL
                     ILAGVGPKSVEQAILERFPWVDLIVRGEAERSAPLLVAALKEKKDLSTVPGISYRHEG
                     RIVHNEKPERITDLDSLHPPAYHHVNLRKYTGFGMITSRGCPYPCTFCSVAPIWDLRS
                     SFRSPESIVEEIKRLHEEGGADLILFQDEFFVSGRERVLRFCNALARSGLDIKWKAFG
                     RVNLTDEDMMHAMEQTGCVEIRFGIESGSDRILELTRKGFTTKQAVEVVSQACTIFRR
                     VDAFYVWGYPFETMDDFYQSVFQMVSFRMLGARILPSLLCLLPQTEIYRRHVDPAKLE
                     FAPDLLPEYMVTGHEVSRHAHIEIRSEHGAVFDFIRSHPDIFPGFFHLELERNIRPKL
                     RILQEFGFYPASAEELAEMESCGAHSPKLNEGQRQLATRAT"
     gene            complement(14139..14420)
                     /locus_tag="PK710_03335"
     CDS             complement(14139..14420)
                     /locus_tag="PK710_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08774.1"
                     /translation="MGRSKMVDDETRTGGGVAPDMPSSAPAASEPQPPPSTSQPSERR
                     EQNPVESESVRPNHSQQPTSLARAIRPDLASERPDAARPGSTPKPSSTK"
     gene            complement(14562..15254)
                     /locus_tag="PK710_03340"
     CDS             complement(14562..15254)
                     /locus_tag="PK710_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08775.1"
                     /translation="MGRSRMFDDENPTGDNSSPRKTIVGGRPPESQTDLPPIPSGIQR
                     LLRYAAQDDGFRSSFLAQRAEAAAAAGIELTASERAILAAVPVTQLEAMLAQVKTQYP
                     KRREFLRQTTASAAVLLGGVAFAQGTKGCDKPAPATRESPRPTHNEMLTEGGAAPSMP
                     LEEPDPSDSQSQPQPEPTAPQPPVRPQQNDTAVGGGIRPDRPNQPLSPTRGIQPDLPP
                     ERPDAAVPRSKP"
     gene            15378..17348
                     /locus_tag="PK710_03345"
     CDS             15378..17348
                     /locus_tag="PK710_03345"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOT08776.1"
                     /translation="MATVFDRLGPHGPLAQSMPGYEHRIGQLQMAEAVEQALEQGQVL
                     ICEAGTGTGKTLAYLVPAICSGQKIVVSTATKALQDQIISQDIPALEQYLGLRPHLAV
                     AKGLSNYLCRRRFEAFRESPHALDRRHRGSLARIESWVRQTETGDVAELEWLPEGDRT
                     WLEVCSSAETRQGSKCPYFRECFVTRMRKRAEAARIVIANHHLVFADLAVRRHSDDHG
                     GALPAYEAIVFDEAHQIEDIASDFFGIRVSTTRLEGLVRDAEKAFRTAGLSDTLLGGK
                     RKKGATLPESARTAGRAFFESVVASVGARQDNAKQTMGRDIWCGETQDRYHRFDASLE
                     ALEAFASKPSSHDDVLAIGTRAHEIRRDLARIIDGQSGSVTWLETRQRSAAVGATPVD
                     VGPFLRETVFERVHACVLTSATLSTSSGFSFFRSRIGLESDFITIREQIVPSPFDFSS
                     HALLYTPTDLCDPNEPNFSQHVAERCAELIRLTDGGALVLCTSNRAMSVIYDKLSQSL
                     SHPLLMQGQAPKINLLDRFKALGNAVLVATMSFWEGVDIAGHALRLVIIDKIPFAVPS
                     DPIVMARCAELEQNGGKPFMDYQVPAAAITLKQGFGRLIRTQRDRGIVALMDRRISQR
                     GYGKSLISSLPPAKRAMAFEQVVAFWQSHREV"
     gene            17478..18020
                     /locus_tag="PK710_03350"
     CDS             17478..18020
                     /locus_tag="PK710_03350"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT08777.1"
                     /translation="MSFGESMGSKNDELSRRLRGLSHAMYAEVQDLLHHPTFLETRWE
                     IHHNVCKSDHLLRSAHYAWMIAVAVRANRRTCARAGLLHDLHSRLGTWSNHGAIAASV
                     ASRLGESKDVCEAIVPHMYPLGPAPKSREGWVLVVADKIASVVDTLHFVAHLITGKSL
                     QKRKELRATDKYMQREVAAN"
BASE COUNT         4242 a         5847 c         5046 g         3635 t
ORIGIN      
        1 gcctcccgtg cctccaccac ccgtgcctcc cgtattcgag cctcccgtgc ctcccttgct
       61 cgggccagca gcaggggacg attcgctgtc atcggaagaa caagcagcca tacccacgat
      121 caggagcggc aatagccaag cgacggtttt catgacctat catgatagca aaatccttgc
      181 gtcgcggggg tgctagcctg ctttggatca aaaaccgcct gcccaacgaa aaacgcccac
      241 catctccaaa ggcagcgctt cggcacccct cagacagtcg gtaaatatat aacaaattca
      301 attagttaac tacagatccc ccaatctggg caggaagctc tcgcgcagga gtctgggcca
      361 ctgggccagc cacggcggaa agggtatcgt cgcaaccgca gcaggcgtcg gcaggcgaac
      421 gggacaagcg cccgaggggg agtgggcaaa ggagtgggta tgggagggag gaatcagaac
      481 cccggaggca tcaagaactc acgcaatcct tgcgtttcac cccggcagtt ttcgttggta
      541 gggttctttt cgtcggggca ccacttgaac gggccgttga ggtagtccat ccacaggtcg
      601 tgatagccgg ggaatcctgg ggcaaaggcg cggtccggtc gggctccacc ccaagaattc
      661 ttcgtacgaa gaagcttgat cttggtgctc ggaagcagag cagcctcgag tttcgcggcg
      721 tcctgaggat tgttcgggtc cagcgtcact cccgcggcaa gcacgccata ctcttccgtt
      781 tcggcttcat agtcttccaa caccgtcatg tggcctccct ggcttcccgg gcgccccata
      841 tccttgagcg tttgcatgtt gaaggagcct cgacgctcat tatgacggtt ttcgagcgcg
      901 ttgaagtcga cattccaggt gattcccacg ggctgtcgcg catgcaacgc tcgctgcatt
      961 cggatgagcg cctcgcgttg gcccgagctg ctgctcggat aacgaacgtt ttgccactcg
     1021 cgaatggctt ccgtaaggtt ggtatccttg tattcgatct tgccgttgac acgcttggcg
     1081 taccggacag gcagcgaatt ggcgtcaatg atattggttc cggcagtgct tgcccccgaa
     1141 cgaaggttct gagagccatc cttgccaaag acctggtcga gctgcgcgcg aacgtcgtcg
     1201 gtcaattccc aagctttatc gagaacttcc cgtacgaggg ctccgtttcg acgcgcctcc
     1261 gaggtggaga gttcaccgtt tttcagagcg gcattgacac gactgagggc cgcggactgg
     1321 cgcgaactca tctcgttttg cgtgtccgcg tacacgaaat ccttgccctt catcaaaccg
     1381 cggtcgcgca tgatggcatt cgacgtccac gtgctgccac ccgtggatac ttcgttatcc
     1441 gacatatatc cgccgatttg atcgaaccaa tgccagtacg tccaatagga ttgggaaata
     1501 tcgagcggtt cgaggtcagg agcaggcccc atcggtggag cattgcacaa cgaagcggca
     1561 cacttgcgct cctcggtcac gtgcttcaca caaatgctat cccacgccgt ttcacagcag
     1621 taggagtctt cttcacatat tttcgccaag caagccgcat gatccgattc cgtgggcatg
     1681 caaccattgg tcagcttctc gccctcttca cagaacaaat gctcgcacgt atgctcccca
     1741 cccgtggcag gctgcgcctc gagataggaa aggttcatcg actccaccca cgtggccgcg
     1801 gcgtaaagcc aacaattgcc aatggactgc cgctccacgg gagtgtgcgc cacatccacg
     1861 atggcttcct gggattgacc aagaatttca ggttcaacag gctgcccttg cgtgcagccc
     1921 acgaagctcg ccaaagcaac gagcgagacc gaagaggtga gacggcgtag agaagttcgc
     1981 gtagccatgg ttctttcctt ttctcctgtc gacatcacca agggttccca acacgctcgc
     2041 tgacgagcaa tgctaccgat tcgtcaatca cggcctgggc ttggtcctgg gccggataag
     2101 gtccgctctg agctatctcc tgaccattgc cagccttcaa actgatgctc accgtgccgt
     2161 cttgctgcgc tacgatgcga taacgctccg gcagcttccc attcgcacga accgactcga
     2221 tgccggtgac cgcattggag cgagaggtat acgtctgaga cgcgagaagg acgcgatcgt
     2281 cttgcgcacg gagttcaaaa cgccacttgg catccgatga ctccttccac agctcaaaat
     2341 gcgctccttt ggaaaccgaa gccttgtgtt gaagaacacc agcgaccaga tctctggttg
     2401 cctgaatgcg cgtatcggct tccgcgcaat cccggaatac cggcccaaac gcaatgacca
     2461 gattgttggc cgcacgaagt tcaaaactac attcgtcggg cccaatcggc gccaccttgt
     2521 aacgctcgag aaatacaccg ttttcttcca cactgagctg cgcgttcaga ctcgccgtct
     2581 tctgtggata cgccccgctc gtgagaaatg catcgccccc aggccccaac aaacggaaaa
     2641 accagtcgcc cgcaggcgtg cgcgacacat cgaaacgccc cggctgctca ggcatgtcct
     2701 gcatcatcaa cccaccaaaa gcagcctcat tcggattatc cgccttccca cccggaccct
     2761 ccgtggggtc ggtctgtcca ccagacgact tatcctcatc cgacgaggac tcgcacgcga
     2821 acatacccaa agccagaacg agggcgacag gagccaaaag caccgtgcgt ccccatccgc
     2881 cgccagacca tccggaattc cgcgtccatc ggctcatcat tggaattacc ctttcatcac
     2941 ctatctcgca gcagcgattg ctgccggtcc ctaagcaatc ctcgttccat cacattaggc
     3001 gtacaggtag gagccaaaac cactgatatc cggcatacac ccacatccta tgtgtactgt
     3061 ccagctccac aatggcgaga aatattcgat cccagtgaga agagcaagtg ctcaatccac
     3121 ctattcgatg aaagtttccg catcgtcaat cccgccccct acacccttac gacacccaga
     3181 atccccccaa aatccgaaac gaccgccatc caccaaagca accgcccccc tttccgagac
     3241 tccccgaatc gcgaacgatg gcttcgatgc ccaacaaaac ccgtacagaa ccatcccttg
     3301 cgacccgaac ccccaccgcg gttgcccttc gccagaatca aatggtccgc ccaaaaacac
     3361 tcacggtaac gtgcaaaccc cagggctcga tggcgaaatg ggcacgcccc actcctcaca
     3421 atggccgctc gtacaatcgg aagcggtcaa gcaatgcgga cggcaacggc tgttggtaca
     3481 cacgtaggca ccttcgtcgc cggccaacac atgacatgca taaatattgt tgcagtcctt
     3541 gccctcggca tcgcgaagac aacgataatc cttcgttttc acgctgaaag agcatcgctc
     3601 ccccgcgtaa caagcgctcg ccttcggatc acattcaacc cccaaatcgc tacaagcccc
     3661 ctgattgcat gttcccttat tcggacatac gccgcaggca tacgggttgt cactgccaca
     3721 gccatacgtg tgcgaaggtc cacaaaccgc cgtaccgcaa tcaggcgtgc aacaaacgct
     3781 ttcttcacag agttgcgaag gcgcacagcc tttgcccagc agtccacatc cgcatcgtcg
     3841 aaccatcggt aaacatatcg cccccttcga agaccccgcg caatctttgt cctcaatgca
     3901 ctccaaacat tgattcgtcg acgtatcaca acgatgagca tccgccggac aatgatcgtc
     3961 ggtcagacag cgtacgcacc gacgagcagc cgcgttgcac accggtgtca tcccgtcgca
     4021 atgatgatcc tgcaagcaag ccacacaagt atgggtggaa atatcgcatg cgggcgcgtc
     4081 ggaggacggg caatcggaat cggataggca cgtcacgcaa cgcccggctt gggaatcgca
     4141 agcccgaatg cttgggggac aatccgccga ttccgaacat agattgcact cttccacacc
     4201 cggaaatgcc ttatggaccg tcacgacccc taccttgcac gccgattcgc acatctccgc
     4261 ctggcccgtc gcccagcacg ttcccttctc gccatacgtg gcgatgaccg tccccagacc
     4321 cgcaggtgtg gttttcgccg tacaagcaat ataggcctca caaatcgcgg ataccccggc
     4381 tgcgccatcg acttcggtga catcgggcaa ggtggccgta tccgacgcct ccaccccagg
     4441 ggatgtgtcg aacagcccac cagcatcgct tcccgtctta cccgacccgc cgtcctctga
     4501 attcccacac gccatgagcc cggaagacac caagaaaagg aggattttgc gcatgataag
     4561 tccatgatat tcgcgccttt tggcaaaaga ggcaaccacc aggtggaaat atgtcgtgag
     4621 gcccgatttc tgccgcgcgt gtgcgttggc caattgggtg aattgccgtt gacagcttga
     4681 aaaaccgcga tagcttcgcg cgattcaatc caggagacgc catgcacgac ccgaaggaga
     4741 gcttgcccgg cctgcagcga aagctgtctc acgccgaaaa agacgatggc ctcgaacccg
     4801 tgatcccttt ggatctcggt tcgatcggca gtttcgacga tcttgcgcga gccatgtcgc
     4861 gcaccgcttt tggcggccga caactcggcg aagcggtcga tgtgctcgtt cggatggcgc
     4921 gggaccccga atgcgccgtg gtactaacct tatccggtgc catgaccgtg gcgaaaatgg
     4981 gcctggtcat ctgcgagatg atcgacaagg gactggtgca agccatcgta tccacaggtg
     5041 ctctcatggc ccacggattc gtggaagcca ccggactcgc gcattataag taccgagagg
     5101 gcatgaacga tgcggaacta tacgaaaagg gatataaccg cgtatacgat acgctcgagc
     5161 ccgaatcgaa cctggagcat gtcgaacata tcctcggcga agtgttcgag cagtgggacc
     5221 cgtccatcac cttgtcttcc cgcaggttga acgcacaact cgggaaatac ctggtccagc
     5281 atcatcccac cgtccggggc gtgctcaaat ccgccgtcga aaaagccgtc cccgtgtttg
     5341 tccccgcgtt ttccgactcc gaaatcggtc tatctctcgg aattttcaat cgtcggcagg
     5401 ctttgctcgg caaacctcgc ctctctttcg accccttcct cgacctcgaa tacttcagct
     5461 ccgcggttgc agtcgtccct cgaatgggta ttttcaccat cggaggcggc gttccccgca
     5521 attgggcaca acaagtcggc ccatacctcg aactgctcga acgacacctg ggtcaagacg
     5581 gccaaggcgg catcgtccgc tatcaatacg gcgtccggat ctgccccgaa ccggcgcatt
     5641 ggggcggact ttccggctgc acctactccg aaggcgtgag ctggggaaag ttcgttcccc
     5701 acgaacgcgg cggccgcttt gccgaggtca tggccgacgc caccatcgca tggcccctgg
     5761 tcgtcaaagc cgccctcgaa cgcctggaca agctcggggg agccgtcccc aaaccagtcc
     5821 tatccggcca cgacatctgg aaaccagcga aatcatagga taaaattgca tccgctcgcc
     5881 ggttccgtat cccccgaaac gagtctatgc tccacagcca tgaatcgaac ctggcctgga
     5941 agaaccgccc cctggatccg ccctcccatc gccgaaatca cgcgtcaggc cgcgcattac
     6001 cgcacccagg gcaaggatct catcgacctc ggccaggcca ttctcggcct cgcacccgcc
     6061 gaaaacgcaa aacaacgcgt tcgcgactat ctcgaaatcc caggtcccca atcctatagc
     6121 cccgacccgg gcctgccgga gcttcgacag gccatcgccc acctgctcat ccatcgcaaa
     6181 gctgtctccg ccgcctgcgc cgaacgggtc atggtcacct gcggcgcaag ccaggccttc
     6241 gccaacgcgc tatttaccgt caccaatccc ggcgatgaaa tcatcacctt cgaaccggga
     6301 tacttcgacc acaattacgc catcgcgatg gcaagctgcc gacaggtccc cgtcgccctt
     6361 cgccttcgcg aaaacagcta cgatttcgac ctggaggcgg tcgtttccgc cttgaccccc
     6421 cacacccgcg ccatcgtgct cgtttccccg gccaatccaa ccggtgccgt ggcttgccca
     6481 tcgttcgtcg agaggctctg cgacctttgc cggtcccgcg acatctggct cctatccgac
     6541 gaaacctatg atttatttac gttttcccca tactccacca cgagtcccgc agcagtggac
     6601 cccacgggca aggtgatggt gctcggctcc ttttccaaaa cgctggggct ggcaggatgg
     6661 cggatcgggt atctttatgg accggaaccg gccatcgagg aagcatataa agtccaggac
     6721 tcgctggtgg tatgcgcaag cgtggcgtcg cagcatgcgg cgttaggagc cttggaatgc
     6781 ctggattcct acgtctcttt cgccagacag gaactgacga aacgcaagga tacgcttgtt
     6841 tcggtacttc gagccgtgag cagcctgcga ccgcgaatgc cccggggagc cacgtttttg
     6901 ctcgccaccc tcgaagacca gcaggatgat atcgcctttt gtcaccgatt gctggcagaa
     6961 gccggcgtga ttgccgtgcc gggctccgcc tttggccccc acggcaaagg gaccatccgt
     7021 ctgtcttttg gcaacctacc ttgtgaatcc attcaaaaag caggcgaaag aatcaaaaaa
     7081 atgggctgaa tccatccccg agccccgtgt caccccccaa aaattcaagg caactccttg
     7141 cctcttccca cgatgatttc gtcaatttag cttcttttcg cggattattg cttgttcgaa
     7201 acgcaaagat cgtgtttcat gtgcctttgc aagacccaag agcctagcga taaggaattc
     7261 gcctctcgaa aggagttccc tggtggctac caagctaagc gaagtacggc agttgtgtac
     7321 gaaacgcgaa attcaactgt tcaagtcggt tcgtcccaca tccatcccaa gctattcccc
     7381 ggctgcattg aagaaaaaga tcgaccgggt gcggacggca caagaaaaat acgagcagtt
     7441 gtcacgtccc cagcggcgaa aggctcgggc cgccgcaagt agcgattccg atccccatgc
     7501 ccaacgcaaa gccgagattt tcggcgaatt gctggaacgc ctcgaggcaa gaagcgctct
     7561 gctcgaagcg cgaaaaaata aaccaaagaa agccgcctcc gccaaaaaga cagtgaaaaa
     7621 ggccgctccc aaaaaggcgg caaaaaagtc cgttgccgcg aaaaaaacca ccaaaaaacg
     7681 aaccacgaaa aaagccgtca aggccacggc ctccaaagcc tccaccataa aatcgcttga
     7741 aaaaccgaaa aagaaagcgg cttccccaat aaccgccaag aaacttgccg ccccgaaaac
     7801 caccaaaaaa cgaaccatca cccccaaagg ccaaggcaaa gccgtcaaaa aggcaaccaa
     7861 agtccagcaa cgaacacaaa gcaaagccgt acgcggccac atcaaagcac aaggcaaacg
     7921 cgcccaggcg cgtcgcgacg ccagacgcta atccacgcgc ctccccctat ggccgttcaa
     7981 taagcgttgg gtcgcgagaa tccctctacg gtcctggcta catccggcta ccgttcgggc
     8041 gggtttgaaa cccgccccta caggccaatg aatgccccat ggcgcgctga ccaatccagg
     8101 ctccctatac gcgcctacgt ccgcgacatt cccgcacgtg aggcgttggg tcgcgagaat
     8161 ccctcttcgg tcctggcaac atccgacatt attgattatt ttataataat ttcatatagt
     8221 tagaacattc atatttccaa ttgtttacgc aactcatcgg cagccgcctg ctctcccgct
     8281 cgttgcaagg cttccaccaa aacatgcttc gctttcggat gcgccttggt ttcaaccgct
     8341 cgttgcaacg aggcaaccgc ctcttgcgga cgatccacag acagaaggca caacccgtac
     8401 aagaacggat cggcccggtc cggcgacaag cgttcgagcc tatcaagcgc ttgttgagcc
     8461 tcccccaact ccaactgcgc catgaccgcc aggtgaatcg cgtgctcccg taactctttc
     8521 gttcgcgcct gctcggccac ctcggaagcc agcgaaagcg ctttttcgaa ttgcccttcg
     8581 acgagagcct gctgcgcttg ctccaggcgc gcatccaatc cccgaccgaa atccttacga
     8641 attccggata gttgcaccaa ttgctttccg gcagaatagc taaaaaacgc aaaaagaacc
     8701 gccgcccacg tcatgtcggc tttccaactg gccagcgcca ccagcggccc cacgatgacc
     8761 gacacccaaa gggctcgaac ccatcccact tccggacctg gaccggataa tacctcacga
     8821 agcaaattgc cgccgtcgag cgggagcacc ggaagcaggt tgaccattcc ccaccccagg
     8881 ttgatccaca gcaacatcag gcccaccgta agcgccggcg tcccttctcg gggttgaacc
     8941 aggagcaaca agacgaacgt gaggccggcc aggacaaagc ccgcgaacgg ccccgcaagg
     9001 gttaccagcg cggaacgaaa acgtgagtgg atatggccgg aaggatacac actgcgtgtt
     9061 agccctccca tcatgtacaa ggtcacctcg ggcgatgcgc caaaagcccg cgccatcaag
     9121 gcatgcccca attcatgaat gagaacgctg aggaacacca cggccatcca aagcacaatg
     9181 gttttcgcgc tgccggacat gccgagcaga agggcgatca accaaaatcc aggctccacg
     9241 cgcacgggaa tgccggctag acgaaattgc agagccagtt gactcatgac agggaatgca
     9301 gggggaaaat gggtttcatg ctcatccgaa ttcgatgcgg ctccacatgt cacggcgcat
     9361 gcgcacacga cgattgtccg agcccgtaat gattttggca acaccccagg ccgccaacaa
     9421 ttccccctcc tgacccaggg ggggaagcac ggaagggccc gtcagaaagc ttcgcgcaag
     9481 cggaccacgc aggggctcgc ccgcaagccc caggtaacca aggggctcca cggaaagaac
     9541 gggtgacatg ggttctgcta cccgcgcggt cccccgcgct tcgattcgat ctacggtcgt
     9601 ccgcttgcca ttgtcataga cccaaaccgg cagaccatcg tgcgtggaat ccacgatgag
     9661 caagtggggc gtcaaaaagg gaagatggtt ttcaagaatg gatacgatga ccgcacgcgt
     9721 ttcgctcact gcaagaacgc ccggcatcgg aagcaggacc tccgccacga gcaataccgt
     9781 cccatcgtcg tcgggcatcg gctgctcggc catcctgtcg caacgctgca gatgaacgac
     9841 cggcaattga gggtccggcg gagcccccgg catccgggga aatacgagcg attcagcacc
     9901 taaggcatcg ggcaagcctt cccgtcgcac cagaagcgat acggtgaatc ggcccgtacg
     9961 ctcggaaacc atcggccaat ctcgctgagc ttttcgcagg attccctttc cttgcgccag
    10021 atgcgcaaga ctttcgcccg tgccatcggt taccacgaac gttgccccca acaccgccgt
    10081 tcccccatcg agaaccaacg cgtgactgtc cttgccattg ggattgatgc tcaccacacg
    10141 atcggaaagc agcacctgcc ctcccaacgc ggtcacccgt tgctggaaaa acgaaatcat
    10201 atcgtcttcc cccgcggcga gcccaaaagg accgcgagac caggcgccgt gcaatcttcc
    10261 gacggcaaac gagggcaagg gacggctacc cgccatgaga ttcgaagcga tctgcgcgga
    10321 ttggaccacg atatccacat agggatgatt ggccgggaat ccgccaagta gcgcatgagc
    10381 gtcttctcgc acgaacggca acgaagaagc cgcggcgttg gtttcacgcc tctcccaaaa
    10441 cgtctccgga ggccagcaaa catccttttc gaacgcggta tccgcctccc cgttgatccg
    10501 tgtcaaatca tggtacaaat catcgatgac ccgtcgcacc tccggaaact cccgatcgat
    10561 ctctcgctga agccattcga gatcgggcgg caactcgaaa cgacggtccg gcagcatcac
    10621 cgaaagcatc ggatccaaag ccacgagacg ccgccgaaac acttgcgatt gcgccaattc
    10681 ggacagtatc cgcttgaacg cgggagatgt ggcagccaac ggcatgaagc aacgtcggcg
    10741 caacacccgg tcgcgaaata cataatccgc gggtctcgcg ccttgtccaa tcaccatgac
    10801 gctgaagtcg cgtcgcgcca gcagcgccgc caacgccatc gcgcccaaag agcgcccgat
    10861 gatgatcaca tcgtaatgct tttcgctcac cgtgcccccg ctacgccaac gctcgcgcga
    10921 accccatcga gttgcaccaa aacacgtccc gacgaaccgt tcgatgccat gatatggact
    10981 cgcccttgcg caatccactg caagactttc ggaaaaagaa cgtgctcccg agccaggatt
    11041 cgggccgaaa gcgtatcgac cgtgtcgtca gcacgcacat cgacgaccga ttgcgcaagg
    11101 atgggtcccg tatccgtccc ggaatccacc aggtggacgg tgcatcccga cacccgaacc
    11161 ccataatcga gcgcctgctg ttgtgcattg atccccggaa acgacggcaa caacgcggga
    11221 tgaatgttga gcacccgatg cggaaacgct tcgagaaagg tcggcgtcaa aagtcgcatg
    11281 aatcccgcca ggaccaccca ctgcaccttc gcctcgcgca tcttcgtcac cagcgcgcga
    11341 tcaaaagctt ctcgcgaatc ataatccttg tgattcacaa tcgcggtcgc cacgcccgct
    11401 cgtcgcgctc gctcgatcgc cagcgctttc ggctggttgg acaccaccaa acagacggtg
    11461 gcatcgaggc gttttttgcg gatcgcatcg aggatcgctt gaagattcga accggaacca
    11521 gaaacgagca cgccgagacg aagaggttcc atgactcacc gaatacaaac ccgggccgac
    11581 tccgccccat gccttggaac gattcgacca aaagaaaacg ctgtctctcc ctcgccccga
    11641 agcacgccaa gcgcctcttt ttccctatcc gacgacacca ccacgcataa ccccaccccc
    11701 atgttgaacg ttcgatacat ctccgactca ttcaccggcc ccacctcggc aagccaacgg
    11761 aaaagcgcag gacgctcata cgcgcccgca tcgagttgcg ctcccaaccc ttccgggata
    11821 atccgcggaa gattgcccac aatcccccct cccgtcacat gacagacccc gcgcaacgac
    11881 gatcccaatg cgctcgccaa cgcgaggatg ctctttgcgt aaatcctggt cggcgtcagc
    11941 aacacgcttc cgagggaagc atctaactca ttgatttttc tatgataatc accatccagg
    12001 gcacgttcta cgaggcgccg ggcgagcgat aacccattgg aatgaatgcc cgatgacgcc
    12061 acaccgatca cggcatcgcc cgcctgcatg cttcgaccat ccatgatgtt gtcgcgctcc
    12121 acgactccga cggcgaatcc ggccaggtcg tagacgcccg gagcatacat cccgggcatt
    12181 tccgcggttt ctcctccgag cagcgcgcag cccgcttggc gacagccttc cacgatgccg
    12241 cggatcacgg cttcccccac atcgacatcc aactgcccgc aagcgaagta gtccagaaag
    12301 aaaaggggac gcgctcccac cgtggcgata tcgttaacgc acatcgccac gagatcgatg
    12361 ccgatggtgt cgtggatgcc aagccgaatt gcgacctcga gtttggttcc cacgccatcg
    12421 gtgccgctga ccaaaaccgg gtttttgtag ccagcgggga tggaacaaag cccggcgaaa
    12481 ccgccgatgc catccagcac ttcgggtacg cgaaccgatt gcgccatcgg cttgatgcgt
    12541 tccaccaatc gatccccggc gtcgatatcg actcccgcat ccttgtagga gataggcatg
    12601 tcccgctcct agccgcttct gccgcggatg tcagcccttc gatcaccagg cgcgtcaggt
    12661 ggcacgagtg gcgagttgtc gttgcccctc attgagtttg ggagagtgag cgccgcacga
    12721 ttccatctcc gccagctcct ccgctgatgc gggatagaag ccgaattcct gcagtatccg
    12781 cagctttgga cgaatgttgc gttccaattc caggtggaaa aatccaggga agatgtcggg
    12841 atgagaacgg atgaagtcga aaaccgcgcc atgctcggaa cgaatctcga tatgcgcatg
    12901 ccggctcacc tcatggcccg tcaccatgta ttcgggcaat agatccggcg cgaattctag
    12961 cttcgcaggg tccacgtgcc gacgatagat ttccgtttgc ggcaacaggc ataaaagcga
    13021 aggcaggatg cgggctccca gcatgcgaaa agagaccatc tggaagaccg actgataaaa
    13081 atcatccatg gtttcaaagg gatagcccca aacgtaaaaa gcatcgaccc ttcggaaaat
    13141 cgtgcaagcc tggctgacga cttccacggc ctgcttggtc gtaaagcctt tgcgcgtcag
    13201 ttctaggatg cgatcggagc cggactcgat gccgaagcgg atttccacgc agccggtttg
    13261 ctccatcgcg tgcatcatgt cttcgtcggt caggttgacg cggccgaaag ctttccactt
    13321 gatatcaagg cccgaacgag ccagggcgtt gcaaaaacgt aggacgcgtt ctcttcccga
    13381 cacgaaaaac tcgtcttgga atagaatgag atcggcgcct ccttcttcgt gaagtcgttt
    13441 gatttcctcg acgatcgact caggggatcg gaagctggag cgcaggtccc agatgggagc
    13501 gaccgagcag aaggtgcagg gataggggca tcctcgggag gtgatcatcc cgaagccggt
    13561 atatttgcgc aaattgacgt ggtgataggc gggaggatgc agcgaatcga ggtcggtgat
    13621 gcgctcgggt ttttcgttgt gaacgatcct tccttcgtgc cggtagctga tgccgggaac
    13681 ggtcgaaagg tctttttttt ctttgagtgc ggcaacaagc agcggcgcgc tgcgttccgc
    13741 ttctcctcgc acgataagat cgacccaggg gaaacgctcg aggatggctt gctcgacgga
    13801 tttgggaccg acgccggcga gaatgagggt tcggtcgggc caggcctgtt tgaattcttt
    13861 catcgcgagc agggtgaaag gcaacaggtt ggccatgcag gaaaagccaa ccaccggcgc
    13921 gggctcccct acgaaagcaa ggatttcctt gctggaaaat gggtcctgcg catcgtgcat
    13981 ctggtagtcg aggtgttgta cctcaaagcc agcagcctcg agcgcggcgg tgacgtatag
    14041 cgtaccgagg gggagatgac gctcgcgttc gatacgatcg aagtatcgga cgaaaagcat
    14101 gttgagattg aggatggtga tatcgggcac gggaaagcct atttcgtaga ggaaggtttc
    14161 ggtgtggaac caggccgtgc ggcatcgggg cgttccgagg caaggtcagg gcgaatggcg
    14221 cgagccagag acgttggttg ctgggaatga ttcggacgta cgctttccga ttcgacagga
    14281 ttttgctctc gccgctctga cggttgcgag gttgatggtg gcggctgcgg ctcggatgca
    14341 gcaggcgcgc tcgaaggcat gtcaggagcc accccacccc ccgtacgagt ctcatcatcg
    14401 accatcttcg aacgtcccat gagaacctcg ctcgacaact gcccgcaaag gctgccccgg
    14461 ccgtgtggtt gacgcaagcc aagggctgcg catttccgtc ttcggattta cacaatctat
    14521 tgtaatatca catagataca agagtcttat ccggcgactg gtcacggttt ggatcggggc
    14581 acggcggcat cggggcgctc ggggggcagg tccggttgaa tgccgcgcgt cggggacaat
    14641 ggttgattgg ggcggtccgg acgtattcca ccaccgaccg ccgtgtcatt ttgctgaggc
    14701 cgcacaggtg gttgcggggc tgtcggctct ggctgtggct gtgactgcga gtccgatgga
    14761 tccggctctt ccaaaggcat gctcggcgcc gccccgcctt cggtcaacat ctcattatgg
    14821 gttggacgcg gggattcccg agttgcaggc gcaggcttgt cgcaaccttt ggtcccctgg
    14881 gcaaacgcca cgcctccgag cagaacggct gcggacgccg tggtttgcct caaaaactcc
    14941 cgacgtttgg gatattgggt tttcacttga gccagcatgg cctcgagctg cgtaacggga
    15001 acagccgcca aaatcgccct ttccgatgcc gtcaactcga tccccgccgc ggcggcggcc
    15061 tccgcccttt gcgcgagaaa cgaagagcga aaaccatcat cttgcgcggc ataacgcaac
    15121 aaacgctgga ttccactggg tatcggcgga aggtcagtct gactctccgg aggcctgccc
    15181 cccacgatcg tcttgcgcgg tgatgaatta tcccccgtcg gattctcgtc gtcgaacatt
    15241 ctcgaacgtc ccatgcgaac ctcgctcgct agctgtctgc aaaggctgct tcactcgtgg
    15301 cagcgaagca aggcaaagag ggatgattgc ggtctttttg ttcgacaatc tcgtcgcccg
    15361 cgagcatgaa tcccaccatg gcgaccgttt tcgaccggct cggcccccac ggacccctcg
    15421 ctcagtcgat gcctggctac gagcatcgaa tcggccaact gcaaatggca gaagccgtcg
    15481 aacaagcctt ggaacaaggc caggtgctga tttgcgaggc cggcacgggc acgggcaaaa
    15541 ccctcgctta cctcgttccc gcaatatgca gcggacaaaa aatcgttgtg tccacggcca
    15601 ccaaggccct gcaagaccaa atcatttcgc aagatatccc tgccctcgaa caatacctcg
    15661 ggctccgtcc ccatctggcc gtcgccaaag gactgtccaa ctacttatgc cgacgccgtt
    15721 tcgaagcctt tcgcgaaagc ccccacgccc tggaccgtcg gcatcgtggt tcgctggctc
    15781 gtatcgaatc gtgggtgcgg caaacggaaa cgggcgatgt ggccgagctg gaatggttgc
    15841 cggagggcga caggacctgg ctggaagtat gctcttcggc ggaaacgcga cagggctcca
    15901 agtgcccgta ttttcgggag tgttttgtga cacggatgcg aaaacgggcc gaagccgctc
    15961 gcatcgtcat cgccaatcat cacctggttt ttgcagacct ggccgtgcga cggcattccg
    16021 acgatcacgg tggtgctctt ccggcgtatg aagcgatcgt attcgacgaa gcgcatcaga
    16081 ttgaggatat tgccagcgat tttttcggga ttcgcgtatc caccacacgc ctcgaaggcc
    16141 ttgtgcgaga tgcggaaaaa gcttttcgaa ccgccgggct atcggacacc ttgctaggcg
    16201 gaaaacgcaa aaaaggggct acgctgccgg agtccgcgcg caccgcggga cgagcctttt
    16261 tcgaatccgt cgtggccagt gtcggcgctc gtcaagacaa cgcgaaacaa acgatggggc
    16321 gagatatctg gtgcggtgaa acgcaagacc gctatcaccg attcgatgct tcgctggaag
    16381 ctcttgaagc tttcgccagc aaaccttctt cccatgatga cgtgctggct attggcactc
    16441 gtgcccacga gatacgtagg gatcttgcac gaatcatcga tgggcaaagc ggatcggtta
    16501 cctggctgga aacgcggcag cggtcggcgg ccgtaggcgc aacgccggtc gatgtgggcc
    16561 ccttccttcg ggaaaccgta ttcgaacgcg ttcatgcttg cgtgctgacg agcgcgacgc
    16621 tgagcacctc gtcgggtttt tcgttttttc gctcacgcat cggactcgaa agcgacttca
    16681 tcaccattcg agaacagatc gtcccctccc cgttcgactt ttcgtcccac gcgttgctct
    16741 acactcccac ggacttgtgt gatcccaacg agccaaactt ttcgcagcac gtggccgaac
    16801 gctgcgccga actcattcgt ctgacggatg ggggcgcgct agtgctgtgt acgtcgaatc
    16861 gcgccatgtc ggtcatttac gacaagctgt cgcagagtct gtctcatcct ttgttgatgc
    16921 agggacaggc acccaaaatc aacttattgg accggttcaa ggcgcttgga aacgctgtgt
    16981 tggtcgcgac gatgagcttt tgggaaggcg tggacatcgc gggccacgca cttcgtctgg
    17041 ttatcatcga caagattccg tttgcggtgc cgagcgatcc catcgtgatg gcccgctgcg
    17101 ccgaactcga gcaaaacggc gggaaaccat ttatggacta tcaggttccc gcggcagcca
    17161 tcacgctcaa acaggggttt ggacggctca tccgcacgca acgagatcgg ggcatcgtgg
    17221 cgctcatgga ccgacgtatt tcgcaacgcg ggtatggcaa gtcgttgatc agcagtcttc
    17281 cgcctgccaa aagggcgatg gccttcgagc aagtggtggc attttggcag agtcaccgcg
    17341 aggtatgaaa aggaacgagc gaggttgcca ttgcacggtt cgcgatgatc gcgttgcaaa
    17401 gttgcaagaa aggtcgttca agtcattgtt ttttcgaatt ccgcgtgacg gcataggccg
    17461 tgcagagtgg cgcgaccatg tcatttgggg aatccatggg gagcaagaac gacgaactca
    17521 gtcggcggct acgcgggttg tcccacgcga tgtacgctga agtacaagat ttgcttcatc
    17581 accctacttt tctcgagacg cgttgggaga ttcaccacaa cgtatgcaaa agtgatcatc
    17641 tccttcgatc cgcgcattat gcgtggatga ttgcggtcgc cgtacgcgcc aatcgtcgca
    17701 cgtgtgcgcg cgctggactt cttcacgacc ttcactctcg gcttggaacc tggtcgaatc
    17761 acggtgcgat cgccgcttct gtggccagtc gactcggcga gagcaaggac gtatgcgagg
    17821 ccatcgttcc gcacatgtat ccgctggggc cggcgcccaa gtcccgggag ggatgggtgc
    17881 tcgtggtggc cgacaaaatc gcgtccgtcg tggatacgct tcatttcgtg gctcacttga
    17941 tcacgggaaa aagcttgcaa aaacgtaaag aattgcgagc cacggacaaa tatatgcagc
    18001 gagaagtagc cgcaaactaa ctcgatcatc cactatcccg atagggtcat tcccgggtcg
    18061 tggccggggt gagccatccc ctcattgttc cccaggcttt tccaccgccc tacgacgact
    18121 ggaaacaagt acctgccatc gtttgggcgc agtcaccgtc atggcgcacg agaaacgttc
    18181 aggggcaccc cctattgatt tgtctgtcca ttcgcttcag aaatagacat tccgagaagg
    18241 cgcatcacca aacagcaaag caactgtccg gaaggcggga gaataggctc aacggataga
    18301 aagatggcga tgcgccggtg gcattccggt ttactgcgtc cttgccacgc aggacgcacc
    18361 cgactatctg cgcactacca tccccactga gcagcaacgg gtcctggctg acgtggaaat
    18421 gacgcgccgt gcgcaggatg aatacctcac cgagcttctg gagtaggctt gataaccttc
    18481 gagggtggac gcatttgata taactagctg tatttacaag tatttttaat aagccgtggg
    18541 ccccatggga ttcacctcac ggttgctgtc aaagaccatg tcaaggggga aagcaggaat
    18601 acgaattaga tattattaag ccaattaata tcattactaa aaccaattaa aacccatcta
    18661 acaacctctg cacccttcca cccacagtgt ggggggaagg gccagggatg gggggccaca
    18721 gcgtgggggg aagggccagg gatggggggc cacactgtgg ggggaagggc
//
LOCUS       DAOSOR010000035        18104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_3108_length_18104_cov_4.477810, whole genome shotgun sequence.
ACCESSION   DAOSOR010000035 DAOSOR010000000
VERSION     DAOSOR010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 18104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18104
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3108_length_18104_cov_4.477810"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PK710_03355"
     CDS             <1..929
                     /locus_tag="PK710_03355"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HOT08778.1"
                     /translation="GVMAGTGIGLGYAAATPAAVKWFGPEKKGLITGIVVAGFGLAPV
                     YIAPLSRSLLAAYGISRSFLIFGVVFLVVATTAAQFMFNPRQNDAGADSKSKASSEKP
                     GEDRTWSEMLRTPAFWTLYAQYACGATAGLMIIGHMAKIVSVQSEGAIQTGFLFVAVL
                     AIFNAAGRVLAGVASDFIGRNATMIIVFLAQAITLFFFSQFTTVTAFLIGSALIGFNY
                     GSCLSLFPATAADLWGTRHLGLNYGILFTAWGVGGVFGPMLAGTIADATGSYSTAYQV
                     ASGLLIVAAVLAAIHLFTKRAAARQSPAPSGS"
     gene            992..1291
                     /locus_tag="PK710_03360"
     CDS             992..1291
                     /locus_tag="PK710_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08779.1"
                     /translation="MPSHRLVAVMGVVLAMFCGCKSEKSTSGRWVACTCPYLTDYDEP
                     AKHTLRVCVPDGENAEETAFGCASKLVHGPAEACRCDPPAEVCDSPKACISNEYK"
     gene            1377..2222
                     /gene="nadC"
                     /locus_tag="PK710_03365"
     CDS             1377..2222
                     /gene="nadC"
                     /locus_tag="PK710_03365"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOT08780.1"
                     /translation="MLLPIHYQNLVEQALLEDLAGGDITTMATVAPDTRAYALAVART
                     SMVACGADVFSCAFRLVDATLHASLLVRDGEEVTAGTTLWRVEGCARSILMAERVALN
                     FAQRMTGIATRTRAFVRAIPPGLPTRITDTRKTTPGLRILERYAVRKGGGHNHRDDLA
                     SAVLIKDNHIVAAGGIPAAVVRARNHAPHTTRIEVEVDTLAQLEEALAAGADIILLDN
                     FSLENTKRAVELTQGKARLEVSGGVNLDQIAAIAQLGIDIISVGGLTHSSPAADIGLD
                     MELLP"
     gene            2219..2995
                     /locus_tag="PK710_03370"
     CDS             2219..2995
                     /locus_tag="PK710_03370"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOT08781.1"
                     /translation="MTPFDIASINKRFSEAEHSYGKPIFYLEETSSTNDEAAILARAG
                     ASHGTVVIADHQRKARGRGGKPWYSEPNTNLAFSLVLRPRVPITHLPAITLVMGLAVA
                     EAVETFVDVPAGIKWPNDVLLRGRKVSGILVESSLSQDRCEYVIAGMGINVLQRSFPS
                     AIEARATSIVLETHRTVTREEVLWQVIMRLALRLESFESGALPELMAEVAKRDEVRGR
                     KVQVGDVQGVADGIDQSGELRVILNGRVQTVRAGEVTFVD"
     gene            3111..4343
                     /locus_tag="PK710_03375"
     CDS             3111..4343
                     /locus_tag="PK710_03375"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HOT08782.1"
                     /translation="MTADISGDNSHALREAYIAFTIRLAWALHRYGAPAHRLEEVLQL
                     MSARFHLPGQFFSMPTGLLFSFGAWENQQTLFLRVEPGDVDLQKQVKLDALAELVALG
                     ELEPREGVEQIEAIVQEPARYGTVLSLACYGLASGAASRFFGGGWREIVVSCTTGLIV
                     GIMAMVMAKRTSTSRVFEPLAAVLSSLLAALGAWWLGSLSTSIAVLGGLIVLVPGLTV
                     TTALNELATRNLMSGTGRLMGAVMIFLVMGVGVALGTQLAEFVPERIGSTIALPAWTE
                     GIALAVGLIAFSVLFRVPMKETPFTFLVGAVIYGIARLATASTGADLAVGLAAFSLGA
                     AANLYARLRRRPSGIVLVPSLMLLVPGSIGFRSLVSLLEHNVLSGVEAAFRMIMVGVS
                     LVTGLLLANLVLPPRRAL"
     gene            4558..5295
                     /locus_tag="PK710_03380"
     CDS             4558..5295
                     /locus_tag="PK710_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08783.1"
                     /translation="MGPRSSRTAWFRAAVVLCAVPILTTSSFAAAQTAKNASKDPCDP
                     TYVPKKGETPPACPAVKDLVLERSDGAKPFAATPPPVEKPPPRAKTTTPPARAIVNSP
                     AFRMQRDGSSKVFVQVHGTPSVRQITTRNGVVFVLTDCRVPVYNNRHALMTHYFNTPI
                     ADARLRQFRNDVHFNIDLRANATPTARLLELVPGQVSVLEVTFPPGHYYESIPSDPPR
                     GRKRTAHGRRSRESRRDGGGVLGPPAP"
     gene            5352..7148
                     /locus_tag="PK710_03385"
     CDS             5352..7148
                     /locus_tag="PK710_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08784.1"
                     /translation="MMGTVLGLGFVAPVSLAQEQPLCLEFQADRAKIDLSTRRLELQG
                     HVSVQAGKYGLHAESLQLGKQEESLQVVGPATLTLCPCDRAPIELDFDRATLEFAGDI
                     TMTSPTLRVGDTPVFWLPWVWFRSWDQPGLLPPKLAWRGDGGLLLGPGFHLPWKDGVG
                     QPAHLDLYISGYTRGGFELESSITTTRSRTNMRFDRMHGDLVRLESEGSQPSAGPGGL
                     TWRVDTVGGSRGVTGLIDIREAYRPFDEAAAHVEVQPSSYSWLYAGVAGTGIRGRDRL
                     HWGPSLLAHAGGALGIHSGWEASGKVRVLDDANQDAAHLTMVRARWFAAPILGPSKIS
                     VDGQSIARSLSLYGHSSMDVVAWSTSEASFPFARSYASTVHLIEPFVRVVGLSSARTA
                     EPSMALLPPAIAGGSRWTATVGIRSDLGPPGASRGFRWEALAGKLGDFEGNTIDSAVV
                     ARAGLVQDWVTIQAQAAVLTRSEYQRAFLLARTSVGGMHSAKANIDIASRSNTDPVDA
                     AAFEPSTQIANPCGVLSQSGTSLSVGGHLPLGSTIRAEVMSDWDLGEKAWLSTGSGLV
                     LEHPCGCLQGRAWVSRRVGREGTDAWLALELQ"
     gene            7270..8964
                     /locus_tag="PK710_03390"
     CDS             7270..8964
                     /locus_tag="PK710_03390"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOT08785.1"
                     /translation="MKTIRPPWSESAPTPGSFRSIAKYGNPRAFKHPSDAWVRMMMQE
                     FGMTEDDFLQRRQEGLESVVVDKPSRLSEAQLNALAAIVGDVNVAVDDYSRVRYASGK
                     TAEEILELRHGVVREIADAVVHPRDKGDVQKLVEFCARERIAITPYGAGSSVNFGCRP
                     YAGGISLALGTHMNAVLEINEVNQTARVQPGILGPAYESALQNAPERFGCQRRYTCGH
                     FPQSFEYSSVGGWIVTLGSGQASTYYGDAYDLVFSQEYVTPAGSFRTIEVPATATGPR
                     INDIMKGSEGAFGVLVEATMKIFRYAPDNRARLGFMYPSWEAAVDASRQIMQGEFGFP
                     AVYRISDPEETDRGLKLYGVPGFADPLLERLGFEPMKRSLCLASVEGDRDYTRLVSRK
                     IKQIAKRHGAISLGSIAMKMWERTRYAEPYMRDDLNDFGILIDTLEAGVTWDNLHRLH
                     QGVRSFVKSRPATMCMTHASHFYPQGTNLYFIFLARMDLEEYVAFQTKIIDKIVENGG
                     TLSHHHGVGRLLAPWMNEHLGVEQMAILRAIKRHFDPDNIMNPGGQLGLDEIPPRR"
     gene            9061..9243
                     /locus_tag="PK710_03395"
     CDS             9061..9243
                     /locus_tag="PK710_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08786.1"
                     /translation="MSQAAIHEGPVYRSKERITPTVGNSIAARGLGCHPRFRGWLLSV
                     LLRALGVGFEPGALVV"
     gene            9272..10096
                     /locus_tag="PK710_03400"
     CDS             9272..10096
                     /locus_tag="PK710_03400"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOT08787.1"
                     /translation="MNFAYEKPTTAQALSRLLEQGGAGASLLAGGTDLLVQLRGRLIE
                     PKLVIDLKGVEELSRLDHHDDGSVTIGATVTANRVIDDKRLSGAMAALPHACSHIAAF
                     AIRNRATIAGNIANASPCADSVPPLCVLDAEVELRTTTATRRLSLPAFIRGVRATVRA
                     KNEYIAAIHVPAQPKGTRSFFRKHQRVRGHDLALANAALLHDPERKRLRVAIGSCSPA
                     PVVVVLDDLFESLDAQEAARRCMAAIIPISDVRASAEYRTDMTGVLVRRLFDDLKA"
     gene            10107..10586
                     /locus_tag="PK710_03405"
     CDS             10107..10586
                     /locus_tag="PK710_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOT08788.1"
                     /translation="MEYDIEVTVNDVRYKARIPASFTLLQLLRDVLHLTGTKEGCGIG
                     ECGACSVVFNGELVNACLVLGVEANGATLRTIEGEAKGEQLSDLQRSFIKHHAAQCGF
                     CTPAMILTAGDLLRRIPTPTDEQIIEGIAGNLCRCTGYEAIVDAIREVARDAKGGAK"
     gene            10583..12961
                     /locus_tag="PK710_03410"
     CDS             10583..12961
                     /locus_tag="PK710_03410"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOT08789.1"
                     /translation="MTVPFHSDKHQYVGGNEERLDAVDKVTGAASYVHEMAIAGMLHG
                     RMKVSPHAHARIKRVDTSKAEKVEGVRAIVTGATLDMTLGLYMCDRPILGRDVVRYQG
                     EQVAAVAAETEYAARRACELIEIEYEPLPAVFDPEEALADNAVRVHPQLHTYNHMRGV
                     FFPQANTNIAHHQKIRKGDIEAGFAQADRIFEHRFTNPPVAHVPIETHVTIAQALPGK
                     RAHIISSAQSPFTVRNLFAIAFGMPHNAVRVQIPYVGGGFGGKAGIHLEPLAYCLSRE
                     AGGRPVKITATREEEFNVLPSRQGLTTYVKTGVKNDGRITALQVKFLWDAGAYADYGV
                     NIGRAAATSGAGPYVVDHCWIDSLVVYTNKLYGTAFRGFGHLEVLWGIERNLDLIAHA
                     LQIDPYEIRQRNVLRAGTTTITGEKVTPNHGRPDLCLETVAKDIGFENRTKASTTGPL
                     PKGKVRGKGIALLQKSPAMPVFTSCSAIVKFNEDASVNVLISGVDYGQGTYSSLANIV
                     AQELSIPRNKVQVVYDCDTATQPYDWQTVASRFQVMGGNACIDACRDCVAQLKQVAGQ
                     VLRAPEHELVCEDERVYVRHSPDQGLSYRELVLGYTYPNGNSIGGPIIGRGKYIAQGL
                     THLDPQTGQGKAALDWTYGAHGVEIEVDTETGELEIINISSAFDVGKVIHEGMCRTQV
                     QGGVLQGLGSALFEGFRFDERGRMLNPSFVDYKIPTAKDKPRQMRQHFIETPQLDGPY
                     GARGVGEHPMISVPSAVGNAIFDATGVDIKDLPLTSERVYMALKAARKAREG"
     gene            12964..14505
                     /gene="fdrA"
                     /locus_tag="PK710_03415"
     CDS             12964..14505
                     /gene="fdrA"
                     /locus_tag="PK710_03415"
                     /inference="COORDINATES: protein motif:HMM:NF004760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HOT08790.1"
                     /translation="MDRLIIRNDAYFDSVFLMSLSAELSETPDLKVGQVVLGTPPNKQ
                     LLQSQGFDATVLDPLGPTDLIVALRADSESTLITAEKRFFELLNRRQPSASHDAIERP
                     IGLDGALRSQPESNLVVISVPGAYATMEAQRALQAGLHVMLFSDNVSVQDEIALKQQA
                     AKRGLLMMGPDCGTAIINGVMLGFANAVRSGPIGLVGASGTGTQEVTCLIHKLGSGVT
                     QAIGTGGRDLTQAVGGITTLCAIDALAADPATRVIVVVSKPPAENVAKQVLDRLAHVN
                     KPSVVHFVGLDRSDKNGSVQFAPDLASAAQAAVQLAEGKPVSMLRSPLPADLLNRARR
                     AKTSKQVALRGLFTGGTMAAEALAHLQAPLGQVLSNLGQGSAPATVVLPHQHAIIDLG
                     GDEYTQGRPHPMIDPSPRAERLMAEAENDRLAVVLLDVVLGTGSHADPAGAMIPAIRQ
                     TQQRAKDRGQHVTVVASVTGTDGDSQGLDGQIAKLRQAEVIVLSSNRDAVRFAHAVID
                     EAFDG"
     gene            14498..15952
                     /locus_tag="PK710_03420"
     CDS             14498..15952
                     /locus_tag="PK710_03420"
                     /inference="COORDINATES: protein motif:HMM:NF018275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HOT08791.1"
                     /translation="MADRSIVDLFGKNLSLINLGLPSFGKDLADQGVSVVDASWQPPA
                     AGDTKTIEALTRFMAWPEAAHRKVNEANALAVKTLREAQPVILDVRTARDVVPGMHDK
                     LVLHAGPPVTWERMCGPMRGGVIGGLLYEGLASTREEAEKLAASGEITFEPCHHHASV
                     GPMAGIMTASMPVWIIENKTFSNRAYCTLNEGLGKVLRYGAFSEEVLTRLRWMRDELA
                     PALSTALAASGPIDLRTVIAQALQMGDEGHNRNRAGTSLVFRLLAPHLVDSALPREVV
                     ARVLRFLDGNDHFFLNLTMPACKSAVEPIRDIPFSTVISTMARNGTDFGVRLAGLVDR
                     WFTAPASKVDGLYLPGFSADDAALDIGDSVITETAGIGGFAMAAAPAIVRFVGGSAAD
                     ALRVSKRMYEISLGEHPVYQIPALDFRGTPTGIDVRAVVEKNVLPAVNTGIAHKEPGI
                     GMVGAGLVVPPVACFRQAFQTFVSVYEEQAKALG"
     gene            complement(16163..17077)
                     /locus_tag="PK710_03425"
     CDS             complement(16163..17077)
                     /locus_tag="PK710_03425"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOT08792.1"
                     /translation="MALGIDIGIHSLRQQENDMKLIDLTIPLGIGTPAWPTYEPLQMK
                     YFKRLAPNGANGQLLTHSNHVGTHLDGEIHFYTPGKDIAELSLDYLVAPGVVVDLSDA
                     VGDYDIYTSKMVEDRADVREGDILIINTGYHKYGWDQPAADEVRYMVQHPGPDREFAE
                     WAKKKKLRWIGVDCGSADHPFNTAIRTWMPRFAKECDRHMQNKYGKTLEEIFEDGKYQ
                     LMHLEMFNHGIIHAECLGGDIDLLNGKRCTIGCFPWRLVDGESCIARIVAFVEDSEYE
                     QLMKVKATRKLTKHGDVVPECTHPRGML"
     gene            17225..>18104
                     /locus_tag="PK710_03430"
     CDS             17225..>18104
                     /locus_tag="PK710_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOT08793.1"
                     /translation="MNAVASPIQSLIQAYRKRSRSPEQEVKERLLHIEKRNPTLLAIR
                     ETFEETALEKARSLPRDSSSIDAMPLYGVPVVIKDSVSRAGKPLRAGRKDAAGAVASQ
                     NALLVDRLEKAGAIVIASANMDELAYGVTGTNPHTGQMRNPRDEQRHPGGSSGGSAAA
                     VADGMVPIAIGTDTAGSVRIPASLCGVVGIRPSHGLLPSQGIAPLAPSLDAPGPIART
                     ARDAALMLSVLADKPNIVSPHPTLTARTAIVLAVKGAFAVDVDPKVQALFEQACHVFQ
                     SITGRLKSETIDALALA"
BASE COUNT         3626 a         5106 c         5414 g         3958 t
ORIGIN      
        1 tcggagtcat ggcgggaacg gggataggac ttggttatgc ggcagcgaca ccggcggcgg
       61 tcaagtggtt tggaccggaa aaaaaaggac tcatcacagg gatcgtggtg gccggctttg
      121 gtcttgcgcc cgtgtatatc gcaccacttt cccgttccct tctcgccgcg tatggtatct
      181 cccgttcgtt tctgatcttc ggtgtcgtgt ttctcgtcgt ggcgacgacc gcggcccagt
      241 tcatgtttaa tccgcggcaa aacgatgcgg gtgccgattc gaaatcgaaa gcatcttccg
      301 aaaaaccggg tgaggatcga acctggtcgg aaatgcttcg aacgcccgct ttctggacac
      361 tttatgcaca atacgcgtgt ggtgcgacag cgggtctgat gatcattggg catatggcca
      421 aaatcgtctc ggtgcaatcg gaaggcgcga ttcaaacggg gtttcttttc gtcgcggtgt
      481 tggccatctt caacgcggcg gggcgtgtgt tggcgggggt agcctcggac ttcatcggtc
      541 gaaatgccac gatgatcatc gtatttttgg cgcaagccat cacgcttttt ttcttttccc
      601 aattcacgac ggtgactgct tttttgatcg gttccgcgct cattggcttc aattacggct
      661 catgtttgtc gctatttccc gcgaccgccg cggacctctg gggaacccgt catctcggtc
      721 tgaactatgg cattctcttt acggcgtggg gcgtgggagg cgtattcggc ccgatgttgg
      781 ccggaaccat cgccgacgcc acgggcagct attcgacagc ctaccaggtc gcttctggac
      841 tattgatcgt ggcggccgta ctggcagcca tccatctttt cacaaagcgg gcggccgcgc
      901 ggcaaagccc agcgccttcg ggatcgtgaa cccacacgct atccctggct atctcggttg
      961 acgaatcctc gtggtggagg atagtcgttt catgccttct catcgcttgg ttgcggtcat
     1021 gggtgttgtg cttgcgatgt tttgcggatg caagtccgag aaatccacct cgggccggtg
     1081 ggtcgcctgc acctgccctt acctcacgga ctacgatgaa ccggccaagc acacgctccg
     1141 ggtttgtgtt ccggatggtg aaaacgcgga ggaaaccgcg ttcggatgcg cttcgaagct
     1201 cgtccatggt cccgccgaag cctgccgctg tgatccgccc gcggaggtct gcgactcccc
     1261 caaagcttgt atttccaacg aatataagta gcgactccca tggcatgcgg gaacgacccg
     1321 caggcttact ccccttccct cttcctgtcc cgatcgcctc gtgtacgctg caccgtatgt
     1381 tgttgcccat tcattatcag aatcttgtcg aacaggcgtt gcttgaagac ctcgcgggag
     1441 gcgacatcac gaccatggcc accgttgcgc ctgacacgcg agcgtatgcc ctcgcggtcg
     1501 caagaacgtc gatggtcgcc tgcggggcgg atgtattctc ctgcgcgttc aggctcgtcg
     1561 atgccacgct gcatgcctcg ttgctcgtgc gcgatggcga ggaagtgacg gcgggaacga
     1621 cgctctggcg tgtggaggga tgtgcgcgca gcatattgat ggccgagcgg gtcgccctca
     1681 atttcgcgca gcgaatgaca ggcattgcca cgcgtacgcg ggctttcgtt cgcgcgattc
     1741 ctccggggtt accgacacgc atcacggata ctcgcaaaac gaccccgggc ctgcgcatcc
     1801 tagaacgcta tgccgttcga aagggaggtg gacataatca ccgcgacgat ctcgcaagcg
     1861 ccgtcctcat caaagacaat catatcgttg cggcgggagg catccctgcc gcggtggttc
     1921 gcgcgcgaaa ccacgcgccc catacgaccc gaatcgaagt cgaagtcgat acgctcgcgc
     1981 agctcgaaga ggcgctcgca gcgggagcag acatcatcct gctggacaat ttctccttag
     2041 aaaacacgaa acgcgcggtg gaactcaccc agggaaaggc gcgcttggaa gtatccggag
     2101 gcgtgaatct cgatcaaata gccgccatcg ctcaactcgg gatcgacatc atcagcgtgg
     2161 gtggtcttac ccactcatcg cccgccgccg acatcggtct cgacatggaa ctgttgccat
     2221 gactcctttc gatatcgcct caataaataa gcgattttcc gaagcagaac actcgtatgg
     2281 caagccgatc ttctacctgg aagaaacatc ttccaccaat gatgaagcgg ccatactggc
     2341 tcgcgctggc gcaagtcatg gcacagtcgt gatcgcggac catcagcgaa aagcgcgggg
     2401 acgtggggga aagccctggt attcggaacc caataccaac ctcgctttct cccttgtgct
     2461 tcgcccccgc gtgcccatca cgcacttgcc cgccatcacc cttgtcatgg gtctggcggt
     2521 ggcggaagcc gtcgagactt tcgtcgacgt ccccgcgggc atcaagtggc ccaacgacgt
     2581 tctgctgcga gggcgaaaag tctcgggaat ccttgtggaa tcttccttgt cccaggaccg
     2641 gtgtgagtac gtgatcgcag gcatggggat caacgtgttg cagcgctctt tcccttcggc
     2701 catcgaagcg agagccacat cgatcgtgct cgaaacccac cggacggtga ctcgcgaaga
     2761 ggtattgtgg caagtgatca tgaggttggc gctgcgactc gagtctttcg aaagcggtgc
     2821 attgccagaa ttgatggcgg aggtagcgaa gagagatgaa gtgaggggtc gaaaggttca
     2881 agtaggagac gtgcaaggtg tggcggacgg catcgatcag agcggtgagc tgcgtgtgat
     2941 tctgaacgga agggtgcaaa cggtgcgggc gggagaagtg acgttcgtgg actgacgttc
     3001 aataatatat tgtatttaca catctttttt gattattcgc cgcccttccg gacccttatg
     3061 cagcaaacgg cgtcggcagg agcaatccgt gctacacgcg cagcgcagtc atgacggccg
     3121 acatttcagg tgacaactcg cacgctcttc gagaggcgta catagcgttt acgattcgtc
     3181 tcgcatgggc cttgcatcgt tatggggctc ctgctcatcg gctcgaggaa gtattgcagc
     3241 tcatgtctgc tcggtttcat ttgccggggc agtttttctc gatgcctacg gggcttctat
     3301 tttcgtttgg tgcctgggaa aaccagcaga cgctattttt gcgggtggag ccgggtgatg
     3361 tggatttgca gaagcaagtg aagctcgatg ctcttgcgga gctggtggcc ttgggcgaac
     3421 tcgaaccgcg tgaaggcgtg gagcaaatcg aggccattgt tcaggagcca gcgcggtatg
     3481 gcaccgtgtt gagtctggcc tgttatggcc tcgcttccgg cgctgcatcg cgtttttttg
     3541 gagggggatg gcgtgagatc gtcgtatcgt gcacgacggg tctgattgtg ggaatcatgg
     3601 cgatggtgat ggcgaagcgc acgtcgactt cgcgcgtctt cgagcctctg gctgccgtgc
     3661 tctcctcgtt gttggccgcg cttggcgcgt ggtggttggg ctcgctatcg acttcgattg
     3721 cggtgctggg aggtttgatc gttttggtcc ccggcttgac ggtgacgact gcgctcaacg
     3781 agcttgccac gagaaacctg atgtccggaa cgggtcgtct gatgggagcg gtcatgatct
     3841 ttctcgtgat gggggtaggc gttgcgttgg gaacgcagct tgcggaattc gttccggagc
     3901 gaattggcag caccatcgca ctaccggcgt ggaccgaggg gattgcgttg gctgtcgggt
     3961 tgatcgcttt ttccgtcctg tttcgggtgc cgatgaaaga aacgccgttt acgtttctcg
     4021 tgggtgccgt gatctacggc attgcgcgac tcgccacggc gagcacggga gcggatcttg
     4081 cggtagggct cgctgcattc tcgctcggag ccgccgccaa tttatatgca aggttgcgtc
     4141 ggcgcccctc gggaatcgta ttggtccctt cgttgatgct ccttgtaccg ggcagtattg
     4201 gttttcgaag tcttgtttcg ttgctcgaac acaacgtgct atccggtgtg gaagccgcgt
     4261 ttcggatgat catggtaggc gtatcgctgg tgacggggtt gttgctcgcc aatctcgtgc
     4321 ttcctccgcg acgcgcgtta tgacgatgat ggtttcggtc tgtggcaaca cgttgagtct
     4381 gaccgtaacg gcaacggcga catgactttc gttttcgcca ccgcttatcg aagcactcgc
     4441 ccccttcgac cgaagcctta tgggccaggg caatggctcg cgccgacgga ccttatcatc
     4501 cgttcagata tcactgagtt ttggcgacaa tccgcggaac gtggtccaat aggcaaaatg
     4561 ggtcctcgtt cttctcgtac cgcttggttc cgtgccgccg tggttctttg cgcggttcca
     4621 attctcacga cttcttcgtt cgcggcagcg cagacagcca agaacgcgag caaggatccc
     4681 tgtgatccga cttatgttcc caaaaaaggg gaaacgcctc ccgcctgtcc agcggtgaag
     4741 gacttggtgc tcgagcggtc ggatggtgcc aaaccgtttg cggcaacgcc gccgccggtg
     4801 gaaaagcctc cgccccgcgc aaagaccacc acgcctccag cgcgggcgat cgtcaattca
     4861 cctgcttttc ggatgcaacg ggatgggtcc tccaaagtct tcgtgcaggt gcatggcaca
     4921 ccttccgtac gtcagatcac aacacgaaac ggagtggttt tcgtactgac cgattgtcgc
     4981 gtacccgtat acaacaaccg tcatgccttg atgacccatt acttcaacac gcccatcgcg
     5041 gatgcgcggc tgagacagtt ccgcaacgac gttcatttca acattgatct ccgagccaat
     5101 gcgacgccga cggcgagatt gttggaactg gttcctggtc aggtatccgt actcgaagtg
     5161 acattcccgc caggtcatta ttatgagtcg attccgagcg atcctccgcg aggccggaaa
     5221 cggactgcgc acggcaggcg atcgcgggaa agtcgaagag atggcggagg cgtgctggga
     5281 ccgccggcac cataaaggga taggacgcca catcgctgat gatcgcatgc cttgcgtgta
     5341 ggcgttggac catgatgggg acggtgcttg gtttgggttt cgtcgcgccc gtttctctgg
     5401 cccaagagca gcctctttgc ctggaatttc aggcagatcg tgccaaaatc gacctttcca
     5461 cccgtcgcct cgagttgcaa ggtcatgtat cggtgcaggc gggcaagtac gggctccacg
     5521 ccgaatcttt gcagctcgga aaacaagaag aaagcttgca agtggttggg cccgccacct
     5581 tgacgttatg tccgtgtgat cgcgcgccca tcgagctgga tttcgatcgt gcaaccctcg
     5641 agttcgccgg agatatcacc atgaccagcc ctaccttgcg ggtgggggat acgcctgtgt
     5701 tttggcttcc ttgggtctgg tttcgttcct gggaccaacc gggattgctc cccccaaaac
     5761 tggcctggag aggggatggg ggccttctgt tgggccctgg gttccatctg ccgtggaagg
     5821 atggtgtcgg acagccagct catctagacc tatacatatc cggatatacc cgaggaggct
     5881 tcgaactgga gtcttcgatc acgacgactc gatcccgtac caacatgcgc ttcgaccgaa
     5941 tgcatggcga tctcgtgcgt ctcgagtccg agggatccca accgagtgct gggcccgggg
     6001 gactcacctg gcgtgtggat acggtgggag gttctcgggg cgtgacggga ttgatcgaca
     6061 tccgagaggc gtatcgtcca ttcgatgaag cggctgctca tgtggaggtt cagccgtctt
     6121 cctattcatg gctgtacgcc ggggtggcag gcacgggcat tcgcggtcgc gatcgattgc
     6181 attggggacc gtcgttgctg gcgcatgcag gaggagccct tggcatccac agtgggtggg
     6241 aggcatccgg aaaggtgcgt gtgctcgatg atgcaaacca ggatgccgct catctcacca
     6301 tggtacgagc gcgctggttt gcggctccca tcctcgggcc ttcgaagata tccgtggacg
     6361 ggcaatccat cgcgcgttca ctatccttat acggacactc atcgatggat gtcgttgcct
     6421 ggtccacctc ggaagcctcc tttcctttcg cgagatccta tgcctccacg gttcacctca
     6481 tcgaaccgtt cgttcgagtc gtgggactat cgtccgcgcg caccgcggag ccgtccatgg
     6541 cgctgcttcc gccagcgatc gccgggggca gccggtggac cgcaaccgtg ggaatacggt
     6601 ccgacctggg tccgccgggg gcgagccggg gctttcgatg ggaggctttg gcgggtaagc
     6661 taggggattt cgaaggaaat accatagatt cggctgtcgt ggcgcgcgcc gggctggtgc
     6721 aggattgggt gacgattcag gcacaagccg ctgtgttgac gcggagcgaa taccagcgcg
     6781 cgttcctcct cgctcgcact tcggtgggag gcatgcattc cgcgaaagca aatatcgaca
     6841 tcgcctctcg ttccaacact gaccccgtgg atgcggcggc gttcgagcct tccacccaaa
     6901 tcgcaaaccc ctgtggcgtg ctatcccaat cgggtacatc tttgtccgtc ggagggcatc
     6961 tcccgctagg ctccacgatt cgagcggagg tcatgagcga ttgggattta ggggaaaaag
     7021 cctggttatc caccggttcc gggctggtgc tggagcatcc ctgcggctgt ttgcagggac
     7081 gtgcatgggt gagtcgtcgc gtgggaaggg aagggacgga tgcgtggctg gccctcgaac
     7141 tccaatgagc aaagaagacc ggaacttcgt ctgtcacgac gtgcgtttgc gtggccgtcg
     7201 tggcgccctt gcttctgatg aagtattttc agttctctcc attcatcttg cgagccgtcg
     7261 aggaggctta tgaaaaccat ccgaccacca tggtccgaat ccgcaccaac acccggctcg
     7321 tttcgctcca tcgcgaagta cggaaatcct cgggcattca agcatcccag cgatgcgtgg
     7381 gtgcgcatga tgatgcaaga gttcgggatg accgaggatg atttcctcca gcgcaggcag
     7441 gaagggcttg aatctgtcgt ggtggataaa ccatcgaggt tatccgaggc gcaactcaat
     7501 gcccttgccg cgatcgtggg ggacgtcaac gtagcggtag atgattatag ccgtgttcgt
     7561 tatgcctccg gcaagacggc ggaagagatc ttggaactca gacacggggt ggttcgtgaa
     7621 atcgcggatg cggtcgtgca tccccgcgac aagggtgacg tgcaaaagct cgtggagttt
     7681 tgtgccaggg agcgtattgc catcacgccc tatggcgctg gttcgtctgt caacttcgga
     7741 tgtcgtccct acgcgggcgg aatatcgctc gcgttgggaa cgcatatgaa cgcggtgctc
     7801 gaaatcaatg aagtcaatca aaccgctcgc gtgcaaccgg gaattctggg tcccgcctat
     7861 gaatctgcct tacaaaacgc acccgagcgc ttcggttgtc agcgtcgata cacatgcggg
     7921 cacttccctc aatcgttcga gtattcctcg gtgggaggtt ggatcgtcac gctcggatcg
     7981 gggcaagcat cgacctatta cggcgatgct tacgaccttg tatttagcca ggagtacgtg
     8041 acgccggcgg gtagttttcg aaccatcgag gtgcctgcaa ccgcgacggg gccaaggatc
     8101 aacgacatca tgaagggctc ggaaggagcg tttggggttt tggtcgaggc cacgatgaag
     8161 atcttccgat atgcgccgga caatcgtgcg cgattgggat tcatgtatcc atcatgggaa
     8221 gcggcggtcg atgccagtcg gcagatcatg caaggcgaat ttgggtttcc ggcggtgtat
     8281 cggatctccg atcccgaaga gaccgaccgc gggctcaagc tctatggggt gccaggattc
     8341 gcggatccgc tgctggaacg attgggtttc gaaccgatga aacgctccct gtgtctggcc
     8401 tcggtcgaag gcgatcgcga ctacacacgc cttgtgtcgc gaaagatcaa acagatcgcc
     8461 aaacgacatg gggcgatatc gctgggaagc atcgcgatga aaatgtggga gcgcacgcgc
     8521 tacgccgaac cttatatgcg cgacgatctc aatgatttcg ggattttgat cgacacgttg
     8581 gaagcgggcg tcacgtggga caatctgcat cgcctgcatc agggcgtccg atccttcgtg
     8641 aaaagtcgcc cggcaacgat gtgcatgacg cacgcatcgc atttctatcc acagggaacg
     8701 aatctatatt tcatctttct cgcccgtatg gacctcgagg agtacgtggc gtttcaaacg
     8761 aaaattatcg ataaaatcgt cgaaaacggg ggtaccctga gccatcatca tggggtggga
     8821 cggctcctgg cgccgtggat gaacgaacat cttggcgtcg agcagatggc aatccttcgc
     8881 gcgatcaaac gtcatttcga tccggacaac atcatgaatc cagggggaca gctcgggctc
     8941 gatgaaattc cgccgaggcg ttgacccatc gatgcggtct cggacaccgt tgcgcggttg
     9001 atcgcgaaaa cgtggtattt ccaggtttat ctcacacgtc gtggggcgaa gggctcgaga
     9061 atgtcgcaag ccgcaatcca cgaagggcct gtgtatcgtt cgaaggagcg aatcacccca
     9121 accgtgggaa attccatcgc tgctcgcgga ttgggctgcc atccaaggtt tcggggatgg
     9181 cttttgtccg tgctgcttcg agcgttgggt gttggtttcg agcctggcgc gctcgtggta
     9241 tgaagcgtct ttcctctctc atggtgccaa catgaatttc gcatacgaga aaccaacaac
     9301 cgcgcaagcg ttgtcgaggt tgctcgagca aggtggggcc ggcgcgagcc ttctcgctgg
     9361 cggaacggac ttgctcgtgc agcttcgagg ccgtctcatc gaacccaagc tcgtcatcga
     9421 tctcaagggt gtcgaggaat tgtcacgtct tgaccatcac gacgatggca gtgtgacgat
     9481 tggagccacg gtgacagcca accgagtcat cgatgacaag cgattgtcag gtgcgatggc
     9541 ggcgcttccc cacgcgtgct cgcatattgc ggcctttgcg atccgcaatc gcgccacgat
     9601 cgcgggaaat attgcgaacg cttctccttg tgccgattcc gtaccgcccc tttgcgtgct
     9661 cgatgcggag gtggagctac gaacgaccac cgcgacgcgt cgtctttcac tacccgcgtt
     9721 cattcgcggt gttcgggcca cggtgcgtgc aaaaaacgaa tacatcgcgg ccattcacgt
     9781 tcccgcgcaa cccaaaggaa cgcgctcgtt cttccggaaa caccagcgcg tgcgaggtca
     9841 tgatctagcc ctggccaacg ccgcgcttct tcatgatccg gagcgaaaac gattgcgtgt
     9901 agccattggt tcgtgcagtc cagccccggt ggtcgtcgtg ctcgatgacc tattcgaatc
     9961 gctcgatgcc caggaagcgg ctcgtcgatg catggccgcc attattccca tttccgacgt
    10021 tcgtgcgagc gcagagtacc gaaccgatat gactggtgtg ctcgttcgtc gtcttttcga
    10081 cgacttgaaa gcgtgaggag gcagcgatgg agtatgacat cgaagtcacc gtcaacgacg
    10141 tgcgttacaa ggcccgaatt cctgccagct tcacgctcct tcagcttctt cgggacgtgc
    10201 ttcatttgac gggcacgaag gagggctgtg ggattgggga gtgcggagca tgctcggtcg
    10261 tattcaacgg cgagttagtc aacgcttgtc tggttcttgg cgtagaggcc aatggggcca
    10321 ctcttcgcac catcgaggga gaagcgaagg gcgagcaact cagcgatctt caacgatctt
    10381 tcatcaaaca ccatgcagcg cagtgtggtt tttgcacgcc agcgatgatt ctgacggccg
    10441 gggatttgct tcgccgcatt cctacgccga ccgatgaaca aatcatcgag ggcattgcgg
    10501 gcaatttgtg ccgatgcacg ggatacgaag ccattgtgga tgccattcgt gaggtagctc
    10561 gcgatgctaa gggaggtgcc aagtgaccgt cccctttcat tctgacaaac accaatatgt
    10621 gggtggcaac gaagagcgtc tcgacgccgt cgacaaggtg acgggagcgg cctcttatgt
    10681 tcatgagatg gccatcgccg gcatgcttca tggccggatg aaagtgagtc cccacgctca
    10741 cgcgcgcatc aaaagggtgg acacatcgaa agcggaaaaa gtcgagggtg tgcgggcgat
    10801 tgtcaccggt gccaccttgg acatgacgct tggattgtat atgtgtgacc gtcccatcct
    10861 tggacgcgat gtggttcgtt accagggaga gcaggtggcg gcggtagcgg cggaaacgga
    10921 atacgcggcg cggcgtgcct gtgaactcat cgaaatcgaa tacgaaccgc tgcccgcggt
    10981 cttcgatccg gaagaagcgc ttgcggacaa tgccgtgcgc gttcatccgc aacttcacac
    11041 ctacaatcac atgcgcggtg tgtttttccc gcaagccaac accaatatcg cgcaccatca
    11101 aaagattcga aaaggcgata tcgaagcagg atttgcccag gccgatcgca tcttcgaaca
    11161 ccgcttcacc aatcctcccg tcgcccacgt ccccatcgaa acccatgtca ccattgcgca
    11221 agcgctgccc ggaaagcgcg cgcacatcat ttcctcggcg caatcgccgt ttaccgtacg
    11281 caacctattt gccatcgcgt ttggcatgcc ccacaacgcc gtacgtgtgc aaattcctta
    11341 tgtaggcggc ggatttggcg gcaaagcggg catccatctc gaaccactcg cctattgtct
    11401 gtcgcgcgaa gcaggcgggc gacccgtcaa aatcacggca acgcgcgagg aagaattcaa
    11461 cgtattgccg tctcgccaag gtctgaccac gtacgtaaaa accggtgtca aaaatgatgg
    11521 acgcatcacc gccctgcaag tcaaattctt gtgggatgcc ggagcgtacg ccgattacgg
    11581 ggtcaacatt gggcgcgctg cggcaacctc cggcgcggga ccttatgtcg tcgaccactg
    11641 ctggatcgat tcgttggtcg tgtacaccaa caagctatat ggcaccgctt tccgcggctt
    11701 tggtcatctt gaagtgcttt ggggcatcga acgaaacctg gatctcatcg cgcatgcctt
    11761 gcaaatcgat ccttatgaga ttcgtcaacg aaacgtgttg cgagccggca ccacgaccat
    11821 caccggcgaa aaagttactc ccaatcacgg aagaccagac ctgtgtctgg aaaccgtcgc
    11881 caaggatatc ggcttcgaaa accgtacgaa agcgagcacc acgggtccgt tgccgaaggg
    11941 caaagtgcgc ggcaagggaa tcgccctatt gcaaaaatcc ccggccatgc ccgtgtttac
    12001 gtcttgctcc gccatcgtca aattcaacga agacgcttct gtcaacgtgc tcattagcgg
    12061 cgtggattac gggcaaggca cctacagctc ccttgccaac atcgtggctc aggaactgtc
    12121 gattcctcgc aacaaagtac aggtcgtcta cgactgcgat acggcgaccc agccttacga
    12181 ttggcagacc gtagcgagtc gctttcaggt gatgggcggc aacgcatgca tcgacgcgtg
    12241 ccgagattgc gtggcgcagc tcaaacaagt cgccgggcag gtgttacgcg cccccgaaca
    12301 cgaactcgtt tgtgaagacg aacgagtata cgtgcgtcat tcgcccgatc agggcctttc
    12361 ctaccgcgag ttggtccttg gctatacgta tccgaatggc aatagcatcg gtggcccgat
    12421 catcggtcgc ggcaagtata tcgcccaggg cctgacccat ctcgatcccc agacgggaca
    12481 aggtaaagcg gctctcgatt ggacgtacgg cgcccatggc gtcgagatcg aagtcgacac
    12541 ggaaacgggg gaactcgaga tcatcaatat ctcatcggct ttcgatgtgg gcaaagtcat
    12601 tcacgaagga atgtgtcgca cgcaagtgca agggggcgtg ttgcaaggat tgggctcggc
    12661 cttgttcgaa ggtttccgct tcgatgaacg aggacgaatg ctcaacccat cgttcgtcga
    12721 ttataaaatt cccacagcca aagacaaacc acgtcagatg cgacagcatt tcatcgagac
    12781 gccacagctc gatggcccgt atggcgcgcg cggcgtggga gagcatccga tgatttccgt
    12841 gccgagcgct gtcggaaacg cgattttcga cgctactggc gtggatatca aggatcttcc
    12901 tctcacctcg gagcgcgttt acatggcgct caaagcagct cgaaaagcgc gggagggttg
    12961 atcgtggatc gactcatcat tcgaaacgat gcgtacttcg actctgtctt tctgatgtcg
    13021 ttgtccgcgg aattatcgga gactcccgac ttgaaagtgg ggcaagtcgt actgggcacg
    13081 ccacccaaca agcaactcct tcaatcgcaa ggattcgatg ctaccgtgct cgatcccctc
    13141 ggcccgacgg accttatcgt ggctcttcgc gcagattcgg aatcgacttt gattactgcg
    13201 gaaaaacgat ttttcgaact gctgaatcgt cgccaacctt ctgcctccca cgatgcgatc
    13261 gaacgcccga tcggactgga cggagcgctt cgttcccagc ccgaatccaa cctcgtcgtg
    13321 atttccgtgc caggagctta tgccaccatg gaggcacagc gagcgcttca ggccgggctt
    13381 catgtcatgc tgttttcgga caacgtatcg gtgcaagacg agatcgctct caaacagcaa
    13441 gccgccaagc gtgggctgtt gatgatgggg ccggattgcg gaaccgcgat catcaatggc
    13501 gtcatgctgg gattcgccaa tgcggtgcgc tccggaccca tcggactcgt gggtgcgtcg
    13561 gggaccggaa cgcaagaagt cacttgcctg attcacaaac ttggctccgg cgttacccag
    13621 gccatcggta cgggaggacg agatctaacc caggccgtgg gaggcattac cacgctctgt
    13681 gccatcgacg ccttggccgc cgatcccgcg acacgggtga tcgtggtggt atccaaacct
    13741 cccgccgaaa atgtggcgaa gcaagtgctc gatcggcttg ctcatgtgaa caaaccttct
    13801 gtcgttcatt tcgtagggct ggatcgatcg gacaaaaacg gctccgtgca attcgcgccc
    13861 gatttggcat cggctgcaca ggccgcggtt caactcgccg aaggcaagcc ggtatccatg
    13921 ctgcgctcac cgcttcccgc ggacctgctc aaccgcgccc gaagggcgaa aacctccaaa
    13981 caggtcgcat tgcgaggctt gtttaccgga ggaaccatgg cggcagaagc cttggctcat
    14041 ttacaagcgc ctttgggaca agtcctatcc aatcttggac agggaagcgc tcctgcgacg
    14101 gtggtgctgc ctcatcaaca tgccatcatc gatctggggg gcgatgagta tacgcaaggc
    14161 cgtccgcatc cgatgatcga tccttcgccg cgcgccgaac ggctcatggc agaagcggaa
    14221 aacgatcgtt tggcggtggt attgctggac gtggtgttgg gaaccggctc gcacgcggac
    14281 cccgcgggtg ccatgattcc tgccattcgg caaacacaac aacgcgcaaa ggacagagga
    14341 caacacgtca ccgtggtggc ttcggtaacc ggtaccgatg gagatagtca ggggctcgac
    14401 gggcagatcg ccaaacttcg gcaagcagag gtaatcgttc tttcgagcaa cagggacgcc
    14461 gtgcgtttcg cgcacgcggt gatcgacgag gcattcgatg gctgatcgaa gtattgtgga
    14521 tttgtttggt aagaatcttt cgttgatcaa cctcggcttg ccttcgttcg gcaaagacct
    14581 ggctgaccag ggcgtgagcg tggtggatgc gtcgtggcaa ccacctgccg cgggggatac
    14641 gaaaacgatc gaagccctca cgcgattcat ggcctggcct gaggcggccc atcgaaaagt
    14701 gaacgaagcc aatgccctgg cggtcaaaac gcttcgagag gcgcaaccgg tcatcctcga
    14761 cgtacgcacg gcacgagacg tggtcccggg tatgcacgac aagctggtgc tgcatgcggg
    14821 tccacccgtg acatgggagc gcatgtgcgg cccgatgcgc ggaggagtca tcggcggatt
    14881 gctctacgaa gggctcgcta gcacgcgcga agaggcagaa aaactcgcgg cttcaggtga
    14941 aattaccttc gaaccttgtc accatcacgc atcggtaggg ccgatggccg gcatcatgac
    15001 agcctcgatg cccgtatgga tcatcgaaaa caaaaccttt tccaatcgcg cctactgcac
    15061 ccttaacgaa gggctcggca aggtacttcg ctatggtgcc ttttccgagg aggtattgac
    15121 tcggttgcga tggatgcgcg acgagctggc gcccgctctt tcgacggcac ttgcggccag
    15181 tggaccgatc gatttgcgca cggtcattgc ccaggctttg cagatgggcg atgaggggca
    15241 taatcgaaac cgagcgggca catccctcgt atttcgtttg ctcgcgcctc atctcgtgga
    15301 ttctgcatta ccgcgagagg tggtcgcgcg ggtgctgcgc tttctcgatg gcaatgatca
    15361 ctttttcttg aacctcacga tgcccgcgtg caaaagcgcg gtggaaccca tccgcgatat
    15421 cccgttttcc acggtgattt ccaccatggc ccgcaacggt acggacttcg gcgtgcgatt
    15481 ggccggattg gtcgatcgct ggtttacggc cccggcatcg aaggtcgatg ggctgtattt
    15541 gccgggcttt tccgccgacg atgccgcttt ggatatcggg gactcggtga tcacggaaac
    15601 cgcgggaatc ggaggcttcg ccatggctgc tgcccccgcg atcgtgcgat tcgtaggcgg
    15661 ttctgccgcg gatgcgcttc gcgtgagcaa gcgcatgtat gaaatatccc tcggagaaca
    15721 ccccgtgtac cagatccccg ccctcgattt ccgcggaaca cccacgggca tcgacgttcg
    15781 ggccgtggtg gaaaaaaacg tgctgcccgc cgtaaacacc ggaattgccc acaaagaacc
    15841 gggaatcggc atggtgggcg ccggtctggt cgtacctccc gtcgcatgct tccgacaggc
    15901 tttccaaacc ttcgtgagcg tgtacgaaga gcaggccaaa gcgctcggct gacaatcgtt
    15961 ttcgcaacgc cccacgcgca ttgtccgcaa tcctccccac tgcacgattt cttccgccct
    16021 cgcgactcgt ttgttgtcat ccgatcgcga ctgaaggccg cacgcatccc aggatttcga
    16081 atcggggatg ggccgtcaac aataggttag ctatttgaaa atattataga aattgggatc
    16141 ggttcgcagg ctcgctcgcc aatcacagca tcccgcgcgg atgtgtacat tcgggaacga
    16201 cgtcgccgtg cttcgtcaac ttgcgcgtgg ctttcacttt catgagttgt tcgtactcgc
    16261 tgtcttccac gaaggccacg atacgggcga tgcatgattc gccatcgacg aggcgccagg
    16321 ggaagcagcc gatggtgcat cgtttgccgt tgagcaagtc gatatctccg ccgaggcatt
    16381 cggcgtggat gatgccgtga ttgaacattt ccagatgcat gagctgatac ttgccgtctt
    16441 cgaagatctc ttccagcgtt ttgccgtatt tgttttgcat gtggcgatcg cattctttgg
    16501 cgaaacgcgg catccacgtt cggatggcgg tattgaaggg atggtcggcg cttccgcagt
    16561 ccacaccgat ccagcgcagc ttctttttct ttgcccattc ggcaaactca cggtcgggtc
    16621 ctggatgctg caccatgtac cggacttcat ccgcggcggg ttggtcccag ccatacttgt
    16681 gatagccggt attgatgatc aaaatatcgc cttcgcgaac gtcggcgcgg tcttccacca
    16741 ttttggaagt gtagatgtcg taatcaccga cggcatccga caggtccacg acgacgcctg
    16801 gagccacgag ataatccaaa gaaagctcgg cgatatcttt tccgggggta taaaaatgaa
    16861 tctcgccgtc gagatgcgtg cccacgtgat tggagtgcgt gagcaattgg ccgttcgccc
    16921 cattcggcgc caggcgtttg aagtacttca tttgaagcgg ttcgtacgtt ggccacgcgg
    16981 gggtgccgat cccgaggggg atggtcaagt cgatgagttt catgtcgttc tcctgctgcc
    17041 gtagcgaatg gataccgatg tcaatcccga gagccatcga tcccacgatg ggacccgcat
    17101 ctcaaaggga gcacgaggca gcatccttcc tgctatgcgg catgtcaagc acgtcggtgt
    17161 tgacctacga cgacgaagct cgcaaccttg ccacctcgtt gaacgccccc atgcggaggt
    17221 acccatgaat gccgtcgcgt cgccgattca gtccctcatc caagcgtatc gcaaaaggtc
    17281 ccgctctccg gaacaggagg tgaaagagcg gcttttgcat atcgaaaaac gcaatcccac
    17341 gctgctggcg atccgcgaaa ccttcgaaga aactgctctg gaaaaggctc gctcgctacc
    17401 tcgagactct tcgtcgatcg atgcgatgcc tctttatggg gttcccgtcg tcatcaagga
    17461 tagcgtgtcg cgtgcgggaa aaccgcttcg cgcgggccga aaggatgcag cgggagcggt
    17521 ggcctcacaa aacgcgttgc tcgtcgatcg actggagaaa gcgggggcga tcgtcattgc
    17581 ctctgccaac atggatgagc ttgcttatgg tgtgaccggc accaacccgc atacgggaca
    17641 gatgcgcaac ccgcgcgatg agcagaggca tccgggaggc tcgagtggtg ggtccgcggc
    17701 cgcggttgcc gatgggatgg ttcctatcgc gatcggtacg gatacagccg gttccgtgcg
    17761 cattcccgct tcgctttgtg gggtggtggg gattcgtccc tcccacggtc ttttgccatc
    17821 gcaaggcatt gcgcccctgg ctccaagcct cgatgcgccg ggcccaatcg ctcgaacggc
    17881 gcgagatgcg gccttgatgt tatcggtgct tgccgacaag ccgaatatcg tgagccccca
    17941 tccgacattg accgcgcgga cggccatcgt tcttgcggta aaaggtgcat ttgcggtgga
    18001 tgtggatccg aaagtccagg ccttgttcga gcaagcttgc catgtcttcc agtcgataac
    18061 gggaagactc aagtccgaga cgatagacgc gctggcgctt gctc
//
LOCUS       DAOSOR010000036        18095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_3114_length_18095_cov_4.454268, whole genome shotgun sequence.
ACCESSION   DAOSOR010000036 DAOSOR010000000
VERSION     DAOSOR010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 18095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18095
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3114_length_18095_cov_4.454268"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2889)
                     /locus_tag="PK710_03435"
     CDS             complement(<1..2889)
                     /locus_tag="PK710_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOT08794.1"
                     /translation="MTDKHDDVIEEDRTQEREKEPSKAEEPDGGVSESEATEGESSVA
                     AKRGEQGSDGGAAAKSSKPSHKRPTRWLRGGVPVGLGGFLAFCIMALEGQYRWGVPVG
                     VVAIAAFSWGLLDLLGTFDDDDETVAHRFRLAQVGPTIAAMAGTLLLTLSLTGLAVAG
                     RMPIWASALSIPASFLGLVVSVYRFGEKIGAWATDETGRPRSLLHRHGFWVVVTGTVL
                     FLPMLGSYSLSDPWETHYGEVAREILARNDWISTWWAQDGWFWSKPVLNFWTEALSMS
                     LFGAGYQPDQMLASFADGRSPWPEWAIRMPVFIFTIVALYFIYKAVANVFGRRAGLLG
                     ALVLSTMPYWYLIAHQTMTDLPFVASMTTSMAFLLLGLHTDPNRTVRTYELDLGVTRF
                     RLSGYHLVLGVIVMTVAPQVFYLLSRNVELLLGGPWGFRPHADVFFSGSAGNCGLPGN
                     QDCTQTFPVNKDMQPAVQALIWAVATGLLLWLNHKERRLSRLFFLAGWFFAAVSTMGK
                     GPAGFALPALCAGAYIFATGKWKKLLDLEFISGLLIILAVAVPWYTAMFMRHGQAFTD
                     RLLFHDMWKRAIVHVHDTNAGVDVSFRYFVWQLGYGLFPWTGLVPVGLVWWLRRREDA
                     AGSGRGDVSVFLAMWFVFAFALFTAMLTKFHHYIFPAVPPAAMLTGIVLDRMMGDEKL
                     IRDKGILGYFILLGGGVLVSIYGLFRCFPGMLMGFKPEGADPRPGSIALGLPLLIVGI
                     ALLVVGIKRFGACGESTKSEPSHGPFENTILGATGIAAALVVGLVGFDLASQAGSSNP
                     GQANLMYLFTYNYKRTWPDSLEFSGMITAFAIAAAVLMLLLVYQRIRAHIVVIATMLS
                     IAWAAWGLNIYLVKTSPHWGQREIIAAYFSHRASPEEQIVAFQMNWKGENFYTSNRIP
                     AFVSSGQKFKDWIKKEKERGVSTMFFVTEHGRARSLESELGEGVASFEK"
     gene            3179..4861
                     /locus_tag="PK710_03440"
     CDS             3179..4861
                     /locus_tag="PK710_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08795.1"
                     /translation="MHPHGSASTTPSPPLVPPPSPSHNPAHLIDDPSVDPPPLAQRFE
                     ALRLDAQDVLRAYSNRAARWSPDLRSIAFLSNRHGAWSAYVSGWKDLRLPPQRIVHAH
                     EVAFVDFGLTSRQILLGASHTENWSFSLFDREQSTVLPCGFDGTPALRSDIARNQFVA
                     LSHDRLLIGEFCSSARRHIPLEPTQRFCGMDHYGQRFLLLDEPATNQATIIEVDARGQ
                     QHLRFDAAGAIRIDQAAYAPDGISLLFSGSASGPPSTVYWVGVRTTQPREIYQVRQAE
                     QRVVEIKTHARSPTFAVLTQGPMGNAMVLVRRSLVGPPLSVPMPAGVGTLDSFSRDGR
                     VLTVTWETPQGPSDIWEIDTARASARKLRDDSRPTLARLDDLLWEKALIESTTGALEA
                     VVYLPEKRPVDGVVVWLGDAEAVGSWRPDIRYWVGEGMGVIEPLAGGLFSRDTLQGWM
                     KGISRWVQERLGSHRVAMVARSSRWKEALDVALPSSWPLVVSPSPPSQEPICVPNGRD
                     ASVLVSVETTNPVSDELVRKLRNAGAHVHYATGDKASRWAREVYFLQTLMTP"
     gene            complement(4848..5657)
                     /locus_tag="PK710_03445"
     CDS             complement(4848..5657)
                     /locus_tag="PK710_03445"
                     /inference="COORDINATES: protein motif:HMM:NF004794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HOT08796.1"
                     /translation="MNFEVQSSQGVSTLWLADEQRRNAMGLSFFSELPKQVEALEADT
                     GTRVVVIAARGPHFSVGLDLKGDLLPQLQGLLGASLASEREKLYALIQSWQKGFRALA
                     SCSKPVIAAVHGACVGGGLDLISACDIRYASADAMFSLRETRLAMVADLGALQRLGGI
                     IGQGHLRDMAFTGRDVTAAEALRMGLVNDVLPTSEAVLARALEMARTIAENSPLAVRG
                     VKRMLHEMNRVAIDRGLEHVALWNTAFLPSEDLMEAFAAFLQKRPPTFKGS"
     gene            complement(5680..6672)
                     /locus_tag="PK710_03450"
     CDS             complement(5680..6672)
                     /locus_tag="PK710_03450"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT08797.1"
                     /translation="MAVTLVLRSDSSDSSITFDQPRVVIGRGEACDFRIPDPSVSHRH
                     ASIRQRGTELVVVDEGSTNGTFVGTTRLHPHSPATLRHGSMVRLGRVWLEIKLESAMP
                     TAQSVQVTKDLALAMVAEAMRREGECMTARLVVIAGPDTGREVPLDEAGQSVVIGRGR
                     DVHFPIDVDDASRRHARVLRRGDLALLRDLGSRNGTLVEGTAIAADRDTVLRPGDQFV
                     ICSDVFVYENPAVEALRQIEALDDELLDSDEWNAVPPPPSTVDIVAEPMPPSVDPPPP
                     SEPKPVIRQAFPRNERRTSTQKKSGWGRTDLIVVFLALVVMVLSTVGMLWLFRT"
     gene            6822..8627
                     /locus_tag="PK710_03455"
     CDS             6822..8627
                     /locus_tag="PK710_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08798.1"
                     /translation="MSFRIPTLLVSLLTVLGILVAHPAHAWVEQTVRRHDARLVLMRN
                     GMARVQHTISLRVNGGPLRSFDVHIADRDVILVGEPTFVLTGGAMDARMPIPVGVEQR
                     PDGPLRVNIDEGKGVRRGIYDFVVTYDVDLLKQNGVTRDGSMLVVSWVGARWDDGIDS
                     VRTTFVVPTTATSPRAVDEVRLDGPNDEVVMAPLGGVLTTLTQYPEVDELEQVRPHVA
                     SGEVVTWKVKVDPAVFGEVQDPRLKAPPPPEAVLIAPERRAAFLAVGGMIAVLYSLLL
                     TLKHRQVVRSSRVRGVHPRPLIPVGIAVRMAFSGPLLAASVGAQVFLEHPFPGAVGVF
                     GVILLSAYRVPEKRVSPRGPGRWLPLSDRDAFEKKEDWPWGWLDAGSPLGKAVFAIVS
                     VLYAVGVWLVSRHATYFAYVAALDFVVIVVLFGTGRRTELPADLVHSAAPLLARVAKE
                     LRKSKKLVAARVRGLGRIPKGTEQPDELRLGVWPKGAIQGFRGIEIGLGWAHAIGQPV
                     ALPQVLLRVLEGSPCHEAVNQRLRKARWIRGRETHERVMVLNPAVPTLDMTVKLAESI
                     SQIVSASTPSPGAQAVSKPTAASRAYVRLCTTGLQ"
     gene            8658..9293
                     /locus_tag="PK710_03460"
     CDS             8658..9293
                     /locus_tag="PK710_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08799.1"
                     /translation="MTVEQPSFDKPLADDESSQQDDSLLLDENAYGIWVSWYDAEEVE
                     YGQSADKFPPRILVFRSAVSDFLEREPLGSAVRAIDFGTAVYVEVGDGDQTVDLMGWV
                     RKLRDFLREGDWMTFAVIAHGGRWVARSAEGQMPARIGDVHVMSFGPSEPFRKVMAAE
                     AMAHDDEETGEPGWGVGLFVEVDALEAMNRKLKNAPTALCFGGACYYRVSA"
     gene            9526..11049
                     /locus_tag="PK710_03465"
     CDS             9526..11049
                     /locus_tag="PK710_03465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT08800.1"
                     /translation="MHSPLLAGAYFSLACWLCVVGIVSLVTHASGRRNVDYLLFSLIA
                     WVVAAQSVACGFCCLRGDLPTQWMLATTFAESASVMAAALHVHFAMRCTGVRRQLEGA
                     AVTYAIAFVLIFMMLHGSGMRAPLSNVGPLTEKEPHTWFATMYVVAIPVFHVIACALF
                     GRSVQRKRRDSLALFTGAVLFALAAVNDMLVGSGVIRGLSLLPLGLIVFSTVLVILFL
                     RRYNLVRQEYAQHAKSLNERGRQLREARRALRELQNELGRKEQLAAIGEMAAVIAHEV
                     RNPLAVITNAVASLKREGLSRHDHDVLLSILEEESERLNRLVSDLLNYARPINLQRQR
                     LVLHEVCQRAMLVGTIHNARLVFDENSARGQIWGDVNLLRQVFDNLVENAVQATDGSG
                     TVTLSIRAASRETAEGFIVTVSDDGEGMDTQVRSRAKDPFFTTRPTGTGLGLAIVNRI
                     VETHGGEMWFESSSGEGTTVNVFLPIGKENSVPPEPRRLIDTLPPRSEPSVYDELKD"
     gene            11104..13014
                     /gene="dnaX"
                     /locus_tag="PK710_03470"
     CDS             11104..13014
                     /gene="dnaX"
                     /locus_tag="PK710_03470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOT08801.1"
                     /translation="MSYLVLARKWRPMTFDDLVGQEHVSRTLSNAIVSGRIAHAFLFT
                     GVRGVGKTTSARILAKSLNCEAGPTPKPCLQCPACTEIAASNDIDVQEIDGASYNGVD
                     EVRRLQESLPYRPSRDRFKVVIVDEVHMLSSNAWNAFLKTLEEPPPHVKFIFATTEVH
                     KVPVTILSRCQRYDFKLISSSSIAKQLRHVLDQEGIAADDAALGLMAREAAGSMRDAM
                     SLLDQAIAWGGDKLVGEEIARVLGIAGRGKLLELAQAMVHGDAQAVLMIVAHLADQGF
                     DLVQVARDTLEVLRDLVVVKVCNSPDSLTDLTPEELATMRALVADARGEDLIRLHHGF
                     STGFDQVVHAPQQRSALEMLLLRLVFRPPLLPVDDLISRLDGIERRLVGRGAALPRSG
                     GPVALRNHRGATAFEPTERTESVSAGDRAVMAARAQPSAAVKDPAPLSRVAAHREIHP
                     VADTEPWVAETVVDSSPIDMEIPVQPTVGTAPSERSQEQPVRRATEPAPTEPAPGSEA
                     AWRAILETVQQQNAALAAVLEHAIPVEISPHAVHLAIEEGSFYADRARDDQAVDLLTR
                     VVRARFHASTEVSLSLLDTGKARGRTLFDINKEQRLEQERKARAELESHKLVQAAIES
                     LGARVHLIKLDE"
     gene            13078..13413
                     /locus_tag="PK710_03475"
     CDS             13078..13413
                     /locus_tag="PK710_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOT08802.1"
                     /translation="MPKTKRFTPPVGDFVRHAASVQKKMDEVRDQLRDREVSAQVDDG
                     NVRVTVTCAGLLRRIEVDPSLVQREGLEMMLDLIVTAANKALDDADRLVDSEVTKATG
                     GLRIPGING"
     gene            13419..14060
                     /gene="recR"
                     /locus_tag="PK710_03480"
     CDS             13419..14060
                     /gene="recR"
                     /locus_tag="PK710_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOT08803.1"
                     /translation="MLPEELFRLSQRFSRLPGVGEKTALRYAMFLLLADPAIARGLGQ
                     ELIALHDNVTPCERCGYVARRSTEGPSLCAICSDHRRDPSLLCVVARVQDLLAIERSG
                     AMRGRYHVLGKLLSPLEGVHASDLPIASIRSRIQNEGIREVLLATPPTVDGEASALFI
                     AQEIGDLEVRISRLASGVPHGGDLEFADQITLGRAIDGRHQVVHDFSEKVVSK"
     gene            14057..14443
                     /locus_tag="PK710_03485"
     CDS             14057..14443
                     /locus_tag="PK710_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOT08804.1"
                     /translation="MTRAIIRTEQAPAAIGPYSQAVRAGDFVFCSGQIPLDPVSGQVV
                     EGDIRVQTEQVMNNLRAVLEAAGGGLDKIVKSTIFLVDLGDFGAVNETYARYFQGDAP
                     ARACVEVSKLPRGVRVEIEATAYLGA"
     gene            14450..15682
                     /locus_tag="PK710_03490"
     CDS             14450..15682
                     /locus_tag="PK710_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08805.1"
                     /translation="MAKKEKKKSGIAESFEPRLLAATASNALEMLEEVGDKAPALVEA
                     WVRESNALAVHAIAWADDAPALARKTARRGLNILKSRGVPLPEKNKKPEPAPQPASFE
                     AWYLAPDQSGVSVFTIGSHSAGQNFDIVDVQIHENAGVIDVRVGEATRSGIRSAFRNI
                     ESSRGYAPVPVPVEWARWHIEQAKKRNATSGLIMPLGFDTSANLLKPVPEQEPVHPIE
                     RENISLDDTEIQSRISGSSTLHNDPEFRSWLPEMEFINELLGKIGHRIGPDPGQQQQE
                     QITAVFNEEVAAATDRFFTPDVRQRLAERLRDAAISVHARAGRERTLDVLATAEAAKR
                     AGLITSPPSEIPFLKDFFNKALALVAASQGGKLNIPIAAPPTEQQGLVAPPEVFEEAA
                     KTRAGRTDSDAESGSASE"
     gene            complement(15770..16705)
                     /locus_tag="PK710_03495"
     CDS             complement(15770..16705)
                     /locus_tag="PK710_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08806.1"
                     /translation="MPPRTHSETRNLVACALCEIIDSVVTPRVRDGILAQALEGFRAE
                     QLPTDPNRLQDFVTGPLREALARTIGPEPVEAMVSDLQRVIDMLLGSTPSLEQRQQPS
                     AFVHDAPHRRSSSRPRMPAVSSSQRSTPIPHHHSTPPRASFRTAPTPRYPSWFPPAAK
                     PISSGRFPRGAAAALSMAGTYSSKPPAESSQPIVFVASHRQDLARSLSLSLASHVQIV
                     ILRNMLELVREFGIAPEGRCVLLIDCQNPSLKPMSVAALADDLPDSLQVVLWGMSPEL
                     RTRLAAISPRCRTWIGIDRETPVDELAGRCTVLVC"
     gene            complement(16935..>18095)
                     /locus_tag="PK710_03500"
     CDS             complement(16935..>18095)
                     /locus_tag="PK710_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08807.1"
                     /translation="LLPEELSTLIHKVFKPRATERSIAFLIDLPDAVVADSEPWAQRR
                     RMVAQWVAGLHKLESFPLEVHCVLYRNAHSNNGDLPEKAWYVSPDLEVDALAPNAESL
                     ANHPAVSFEQIFRSHPLLVAPTEFSATAPLKIAARTFPFRAATMPGFSPSMIPALRLD
                     YEEINRRVLLLKSLLDEAVSATCRFLVDQSTERLLRLDLRHRTAHASGGVFPDPGVAG
                     NLPSGESYIVPYEGERKADPSQSEGLLPVELDGDIVTYRISHNRAVEVIDAEQSEQGR
                     REAKRIAEEPAYANIAELGLGVLDAFGVEPSGEILLDEKLGLHIAFGRSDHFGGIVGP
                     KQFSGPDAVVHIDRVYIPRIQPRVKVLEVQLIRENMPPLRIMKDGAYTIDFS"
BASE COUNT         3882 a         5138 c         5303 g         3772 t
ORIGIN      
        1 tttctcgaaa gaggccaccc cttcccccaa ttcgctttcg agacttcggg ccctgccatg
       61 ttcggttacg aagaacatgg tggacacgcc gcgttctttt tcttttttga tccagtcttt
      121 gaacttctgg ccgctggaga cgaaggctgg aatgcggttg ctcgtgtaaa aattctcgcc
      181 tttccaattc atttggaagg caacgatttg ctcttcgggg ctggcgcgat gcgagaagta
      241 cgcggcaatg atctcccgtt gtccccaatg gggggatgtt ttcacgaggt agatgttcag
      301 cccccaggca gcccaggcga tgctgagcat ggtggctatc accacgatat gtgctctgat
      361 tcgttgatac acgagcaaga gcatgagcac ggccgcggcg atggcgaacg cggtgatcat
      421 gcctgaaaac tcgagggaat ccggccaggt acgcttgtag ttgtacgtga acaagtacat
      481 cagattcgcc tgtcctggat tgctcgagcc agcttggcta gccaggtcga aaccgaccag
      541 gccgacaacg agagccgcgg cgatgcccgt ggctcccagg atggtgtttt cgaacgggcc
      601 gtgggacggc tccgatttcg tggattcgcc gcatgcaccg aagcgcttga tgcccacgac
      661 aagcagggcg attccgacga tcaacagagg caatccaaga gcgatcgaac caggccgagg
      721 gtctgcccct tccggtttga atcccatgag cattcccgga aaacatcgaa acagtccata
      781 tatggatact aggacccctc ctcccaacaa gatgaaatat cctagtattc ccttatctct
      841 tatcaacttt tcatcaccca tcatccggtc aaggacaatt cccgtcaaca tggccgccgg
      901 ggggaccgcc gggaaaatgt agtgatgaaa cttcgtcagc atggccgtaa acaaggcaaa
      961 agcgaacacg aaccacatgg caagaaatac cgacacgtcg cctcgtcccg aaccagccgc
     1021 atcctctcgt cgtcgcaacc accaaacgag tcccacggga acgagacctg tccaggggaa
     1081 aagcccatag ccgagctgcc acacgaaata gcgaaacgaa acgtcgacgc ccgcgttggt
     1141 atcgtggaca tgcacgatgg ctcgcttcca catatcgtgg aagagcaagc ggtcagtaaa
     1201 cgcttgccca tgccgcatga acatcgccgt gtaccagggg acagccaccg caaggatgat
     1261 gagcagtcca ctgatgaatt cgaggtcgag cagttttttc cacttgcccg tggcaaagat
     1321 atacgcgccc gcgcaaagcg ccggcagcgc aaagccggcc ggcccctttc ccatggtcga
     1381 caccgctgca aaaaaccatc ccgccaagaa aaacagccga ctgagccttc gctccttatg
     1441 attcaaccaa agcaacaatc ccgtcgcaac agcccagatc agcgcttgca ccgcgggctg
     1501 catatccttg ttgaccggaa aggtctgcgt acaatcctga ttgcccggaa gcccacaatt
     1561 gcccgcggac cccgaaaaaa atacgtccgc gtgcggccga aatccccaag gcccacccag
     1621 caacagttcg acattgcgcg acaataggta aaacacctgg ggcgccaccg tcatcacaat
     1681 gactcccaac accaggtgat acccagacaa acgaaaacgc gtcacaccca gatcgagttc
     1741 gtaggttcgt accgtgcgat tcggatcggt gtgcaaacca agcagcaaaa atgccatcga
     1801 tgtcgtcatc gacgccacga aaggcaaatc cgtcatcgtc tgatgcgcga tgagatacca
     1861 ataaggcatc gtcgaaagaa ccagagcccc gagcagtcca gcccttcgcc cgaagacgtt
     1921 ggccaccgcc ttgtagatga aatagagcgc aacgatcgta aagatgaata cgggcatgcg
     1981 aatggcccac tccggccacg gcgatcgccc atccgcaaaa gaagccaaca tctggtccgg
     2041 ctgatagccc gcgccaaata gcgacatcga tagcgcttcc gtccaaaaat tcagcaccgg
     2101 tttggaccaa aaccaaccgt cctgcgccca ccaagtcgaa atccaatcat tgcgcgccag
     2161 gatctctcgg gccacctccc cgtaatgggt ttcccacggg tcactcagcg aatagcttcc
     2221 caacatgggc aaaaacaaaa ccgttccggt cacgacgacc caaaacccat gtcgatgcag
     2281 tagagagcgc ggacggccag tctcatccgt tgcccaggcg ccaatttttt caccgaatcg
     2341 ataaaccgat accacgaggc ccaaaaacga agccggaata gataaagcac tcgcccagat
     2401 cggcatccgt cccgcaaccg caagccccgt caacgacagg gtcaacaata gcgtccccgc
     2461 catcgccgcg atcgtgggtc caacctgcgc caaccgaaaa cggtgggcca ccgtctcgtc
     2521 atcatcgtca aatgtgccca gcaggtcgag aagcccccat gaaaacgccg caatcgcaac
     2581 cactcctacc ggcacacccc accggtactg cccttcgagg gccataatgc aaaacgcaag
     2641 gaaaccgccc aatccaacag gcacgcctcc tcgaagccaa cgagtgggac gcttgtggga
     2701 cggcttcgag gacttggccg cagcaccacc atccgagccc tgctcgcctc gtttcgcagc
     2761 cacgctcgat tcgccttcgg tagcctcgga ttcgctcacg cccccatccg gttcctccgc
     2821 cttcgaaggt tccttttctc gctcttgcgt tcggtcttcc tcaatcacgt cgtcgtgttt
     2881 gtcggtcatc ttattcgctc gatggtcaag acccggcttt gccacaccag caacgccgtc
     2941 ggcacggacc gcgacgtgtt agcaacaatg gccgcgggtg accagacttg gcccgctatc
     3001 cctgcacatt cgtggctcag gccatgctga tttatgatac gccgatgccg tggagccaac
     3061 gcgctttgtt ccaacgcccc gagatcaagg taacatacga ttattattat tgtttttaca
     3121 gatcccgagc gtcctcttcg cggcacttcc ttcctgccac tttcccgcct tctgcgccgt
     3181 gcatccccac ggctctgcgt cgacaacccc ttcacccccg ctcgttccac caccatcacc
     3241 aagccacaac cccgcacatc tcatcgatga cccttctgtc gacccacctc ccctcgcaca
     3301 acgattcgag gcccttcgcc tcgatgcaca agacgttctt cgggcctact cgaatcgcgc
     3361 cgctcgatgg tccccggacc tgcgttcgat cgcattcctt tccaatcgtc atggcgcatg
     3421 gagcgcgtat gtatcgggat ggaaagacct tcgccttcct ccccaacgca ttgtccacgc
     3481 ccacgaggta gccttcgtcg acttcggtct cacctcccgg caaatccttc ttggcgcatc
     3541 ccatacggaa aactggtctt tcagcctttt cgaccgggag cagtccacgg tgctgccttg
     3601 cggattcgac ggcacccctg cacttcgctc ggatatcgct cgcaaccaat tcgttgcctt
     3661 gtcccacgat cgattgctga taggcgagtt ttgcagctcg gccaggcgcc acattcccct
     3721 cgaacccacg caacgttttt gcgggatgga ccattacggg cagcgttttt tactgctcga
     3781 tgagccggca acgaaccagg caacaatcat cgaggtggat gcccggggac agcaacacct
     3841 tcgattcgat gcagcgggag ccattcgtat cgaccaagcc gcctacgcgc ccgatggcat
     3901 atctctgcta ttttcgggga gcgcatccgg tccgccaagc acggtgtatt gggttggcgt
     3961 acgcaccacg cagcccagag agatttatca agttcgacaa gcagaacagc gagtggtgga
     4021 aatcaaaacg catgcacgaa gtcccacgtt cgccgttctc acgcaaggtc ccatgggaaa
     4081 cgccatggtt ctggttcgaa gaagtctcgt ggggcctccc ctttcggtgc ccatgcctgc
     4141 cggggtagga acgttggaca gtttttcgcg tgatggccgg gtgttaacgg tgacgtggga
     4201 aacgccacaa ggtccttctg acatttggga aatcgatacg gctcgggcgt ccgcgcgaaa
     4261 attgcgtgac gacagccgtc caacgctggc gcggctcgac gatctcctgt gggaaaaggc
     4321 gctcatcgaa agcacgacgg gagccctgga agcggtggtt tatctgccgg aaaagcgccc
     4381 cgtggacggt gtcgtggtgt ggctgggaga cgcggaagcc gtgggcagtt ggcggccgga
     4441 tatccggtat tgggtgggag aaggcatggg cgtcatcgaa ccactggcag gcggactttt
     4501 ttcgcgagat acactgcaag ggtggatgaa agggatttcg cgatgggtgc aagagcggtt
     4561 gggcagccat cgggtggcga tggtcgcgcg ttcatcccgc tggaaagaag cgctcgacgt
     4621 tgccctgccc tcttcctggc cgcttgtcgt ttcaccttcg ccaccaagtc aggaaccgat
     4681 ttgcgtgccg aacggtcgcg atgcttcggt actcgtgagc gtggagacga cgaatccggt
     4741 ctccgatgaa ctggtgagga aactgcgaaa cgcgggagcc cacgttcact acgcgacggg
     4801 cgacaaggcc agccgatggg ctcgagaagt ctattttttg cagaccctca tgaccccttg
     4861 aaggttggcg ggcgcttttg caaaaacgcc gcaaacgctt ccatcaggtc ctcgctcggt
     4921 aaaaatgcgg tattccacaa cgccacgtgt tccaaccccc gatcgatggc cacacggttc
     4981 atttcatgaa gcatccgttt cacgcctcga accgccaatg gactgttttc ggcgatcgtt
     5041 cgcgccattt ccaaggcacg agccaagacc gcttccgatg tgggcagcac gtcattgacc
     5101 aaccccatgc gcaaggcttc ggccgcggta acgtcgcgtc cggtaaaagc catgtcccga
     5161 agatgcccct gaccgatgat accgccaagc cgctgcaacg cccccaagtc cgcaaccatg
     5221 gcaagacgag tttcgcgcag actgaacatg gcatccgcgg acgcataacg aatatcgcaa
     5281 gcagagatca ggtcgagtcc accgccgaca caggcaccgt gaacggcggc gatgaccggc
     5341 ttggagcaag acgccagcgc tcgaaatccc ttctgccacg actgaatcaa ggcgtagagc
     5401 ttttcgcgct cggaagccaa gctggccccc aacaatccct gcaactgcgg aaggaggtcg
     5461 cctttcaagt ccaaaccaac ggaaaaatgc ggtccgcgag cggcaatcac cacgacacgc
     5521 gtacccgtat ccgcctccaa agcttccact tgtttgggca gttccgaaaa aaacgaaagg
     5581 cccatggcat tgcgacgctg ctcatccgca agccaaaggg tcgatacccc ctgtgagctt
     5641 tgcacttcga aattcattcc gttttccttt cccgccgact cacgttcgaa acagccaaag
     5701 cattcccacc gtgctcaata ccatcaccac cagggcaagg aacacgacga tcaaatccgt
     5761 ccttccccat cccgacttct tttgggtaga agttcgacgc tcattgcggg gaaaagcttg
     5821 tcttattacg ggcttaggct ccgacggggg gggcggatcg acactcggag gcataggctc
     5881 cgccacgata tctaccgtcg acggaggagg aggtaccgca ttccattcgt cactgtccaa
     5941 aagctcgtcg tccaaagcct cgatttggcg caacgcctct acggccgggt tctcatacac
     6001 gaatacatcg gaacaaatga cgaactgatc gccaggacgc aacaccgtat cacggtcggc
     6061 tgcaatggcg gttccctcga ccaacgtacc attgcgggac cccaaatcac gcagcagtgc
     6121 cagatcaccc cggcgaagaa cccgcgcatg acgtcgtgac gcatcatcca catcaatggg
     6181 aaaatggaca tcacgaccac gaccaatgac cacggactgc ccggcttcat ccaaaggcac
     6241 ttctcgtccc gtgtcggggc ccgcaatgac caccagacgt gcggtcatgc attccccttc
     6301 acgacgcatc gcttcggcca ccatcgccaa cgccaaatcc ttggtgactt gcacggattg
     6361 cgcggtcggc atcgcggact ccagcttgat ttccagccac acgcgcccca gacgaaccat
     6421 gctgccatgc cgcaatgtgg cgggagaatg cggatgcaac ctcgtggttc ccacgaaggt
     6481 gccgttcgtg ctcccctcat cgacgacgac gagttcggtt cctcgctgac ggatgctggc
     6541 gtgtcgatgg ctgacggatg ggtccgggat tcgaaaatcg cacgcctcac cgcgtcctat
     6601 gacgacccgc ggctgatcga acgtgatgct ggagtcggaa ctatccgatc gaagtacgag
     6661 ggtgaccgcc attcgttgcg cttctcgtcg ctgtatagtc aggactagac caagggctca
     6721 acctgggaaa tctcatttct cgcgggaatc ggggcgatcg ggaattggga tgggagatta
     6781 tttggccagt tggccaagcc aaggtaatcc catgtaggac aatgtcgttt cggattccaa
     6841 cgctcctcgt ttcgcttctc acggtgctcg ggatccttgt tgcgcatccc gctcacgcct
     6901 gggtggaaca aaccgttcgt cgtcatgatg cccgattggt gttgatgcgc aacgggatgg
     6961 cccgtgtgca gcacacgata tccttacggg tgaacggcgg ccctttgcgt tcgtttgacg
     7021 ttcatatcgc ggatcgggac gtgattttgg tgggggaacc gacctttgta ttgacgggtg
     7081 gcgcgatgga cgcgcgaatg cccattccgg tgggggtgga gcagcgtccg gatggtcctc
     7141 ttcgtgtaaa catcgatgag ggcaagggag tgcgtcgggg catctacgac ttcgtggtga
     7201 cgtacgatgt ggatttactc aaacaaaacg gggtgacgag ggacgggtcg atgctggtcg
     7261 tgtcctgggt gggggcacga tgggacgatg gcattgacag tgtgcgaacg acatttgtgg
     7321 ttcccacgac ggccacgtcg ccgagggcgg tggacgaggt acggttggat ggtccgaacg
     7381 atgaggtggt gatggcgcct ttggggggag tattgacgac gttgacgcag tatcctgagg
     7441 tggacgaatt ggagcaggtg cggccgcacg ttgcctcggg ggaggttgtt acgtggaagg
     7501 tgaaggtaga tccggcggta tttggggagg tgcaagaccc acggttgaag gctcctccac
     7561 cgccggaagc ggttctgatc gctccggagc gtcgagcggc gtttttggcc gtgggcggta
     7621 tgattgcggt tttgtattcg ttgctgttga cgctcaagca taggcaggtg gtgcgaagca
     7681 gccgtgttcg aggggtgcat cctcgtccac tgattcctgt gggtatcgcg gttcgcatgg
     7741 cgttttcggg gccgttgctg gcggcttcgg tgggagcgca ggtttttttg gagcatccgt
     7801 tccctggggc ggtgggcgtg tttggcgtga ttctgctcag cgcgtatcgc gtaccggaaa
     7861 aacgggtatc ccctcgaggt ccgggccgat ggctcccgct ttcggatcgg gatgctttcg
     7921 agaagaaaga agactggcca tggggatggt tggatgcggg atcgcctttg ggcaaagctg
     7981 tatttgcgat cgtgagcgtc ctgtacgcgg tgggagtatg gctggtttcc cggcatgcta
     8041 cctatttcgc ttatgtggca gcgctggatt tcgtagtgat tgtcgtgttg tttggaacgg
     8101 gtcgtcggac ggaactcccc gcggacctgg tgcattctgc ggctccgttg ctggctcggg
     8161 tggcgaagga gcttcgcaag tcaaaaaaac tagtcgccgc gcgtgtgcgg ggtcttgggc
     8221 ggatcccgaa gggaacagag cagccggatg agttgcgcct tggcgtgtgg ccgaaaggcg
     8281 ccatacaggg atttcgcggt attgaaattg gattgggatg ggcccacgcc atcggccagc
     8341 ccgtggctct tccccaagta ttgttgcggg tgctagaagg ttccccatgc catgaagcgg
     8401 tcaaccagcg gttgcgcaag gcgcgttgga ttcgtgggcg agagacgcac gaaagggtga
     8461 tggttctcaa tcctgcggta ccgacgttgg atatgacggt gaaattggcg gaaagcattt
     8521 cgcagattgt cagcgcgtcg acgccttcgc ctggcgccca agcggtttcg aagcccaccg
     8581 ccgcttctcg ggcttacgtg cggctatgca caacgggctt gcaatgaagc gccaatgctg
     8641 gaattcattc cgtcatcatg acggtggaac aaccgagttt cgacaagcca ttggctgacg
     8701 acgaatcgtc gcagcaggat gattccttgc tcctggacga gaatgcttat ggaatatggg
     8761 taagttggta cgatgcggag gaagtcgagt acgggcaatc ggcggataag tttcctcccc
     8821 ggatccttgt gtttcggtct gccgtgagcg attttctcga acgggaaccg ttggggtcgg
     8881 cggttcgggc catcgatttt ggtacggctg tctatgtgga ggtgggcgac ggcgatcaga
     8941 cggtggattt aatgggttgg gtgcgcaaac ttcgagattt ccttcgtgag ggcgattgga
     9001 tgacgtttgc ggtcatcgcc catggtggac gttgggtagc tcgatcggca gaggggcaaa
     9061 tgccggcgcg gataggtgat gtacacgtga tgagttttgg tccaagcgag ccatttcgca
     9121 aagtgatggc ggcggaggcc atggcgcacg acgatgaaga gacgggcgaa ccgggctggg
     9181 gagtgggctt gttcgtcgag gtcgatgcgc tcgaagcgat gaaccgcaag ttgaaaaacg
     9241 cgcccacggc cctgtgtttc gggggtgcgt gttactatcg ggtgagcgct tgatcacatc
     9301 cgacaggaca attcccacgg gctgattcat ccaacccacc ccaccgcacc ataatgcaag
     9361 cccctgctca tcctggcttg aaaaccgcac gaacgaggat tagacgctag ccatatcccg
     9421 attccatcat ggttatcgga atgaatcgtc gaagcagacg tggcggaaat ggtatcatac
     9481 ctctgacctt caccctgccc aacactcgct gacaggatct catcggtgca ttccccgttg
     9541 cttgcgggtg catacttctc cctggcgtgc tggttgtgcg tcgtggggat cgtgtctctc
     9601 gtgacccacg catcgggaag gcgaaacgtt gactatcttt tgttcagcct gatcgcctgg
     9661 gtggtcgcag ctcaatcggt ggcctgtggg ttttgctgtc tgcgaggaga tcttccgacg
     9721 cagtggatgc tagcgaccac gttcgcggaa agcgcctcgg tgatggcggc tgcgttgcac
     9781 gtccatttcg ccatgcgatg cactggcgtg cgtcgtcaac tcgaaggggc cgctgtcacg
     9841 tacgcgatcg ctttcgttct catttttatg atgttgcatg gttcggggat gcgggccccg
     9901 ttatcgaatg tgggtccttt gaccgaaaaa gagcctcata catggtttgc aaccatgtac
     9961 gtggttgcaa tacctgtctt tcatgtcatc gcttgtgcgt tgttcggtcg gagcgtccaa
    10021 cgaaagcgac gggacagttt ggcgttattc actggagccg tgctattcgc gctggcggca
    10081 gtcaacgata tgttggtggg ctcgggggtc attcgtggtc tttccttgct tccccttggg
    10141 ttgattgtat tcagcacggt gctcgtcatc ctttttcttc gtcggtacaa cttggtacga
    10201 caagagtatg cgcaacacgc gaaaagcttg aacgagcggg ggaggcagct tcgagaagcc
    10261 agaagggcgc tgcgagaatt acaaaatgag ttgggtcgga aagagcaact cgcggccatt
    10321 ggggagatgg cggccgtcat cgcgcatgaa gtgagaaacc ctctggcggt cattaccaac
    10381 gccgtggcga gtttgaagcg cgaagggcta tcacgtcacg atcacgacgt attgctttcg
    10441 attctcgaag aagaatcgga gcgtctcaat cggctggtat cggatttgct caattacgcg
    10501 cggccgatca accttcagcg gcaacggctg gtacttcacg aagtgtgtca aagagccatg
    10561 ctcgtaggga cgattcacaa tgcgcgtttg gtgttcgacg aaaacagcgc ccgtggtcag
    10621 atctggggcg atgtgaactt gcttcggcaa gtatttgata atcttgtaga aaatgcagtt
    10681 caagcgacag atggttcggg gacggttacg ctatcgattc gagcggcgtc acgggagacg
    10741 gcggagggat tcatcgtgac ggtatccgat gatggagagg gaatggatac gcaggttcga
    10801 agtcgagcca aggacccgtt tttcaccact cgtccgacgg gcacgggtct tgggttggcg
    10861 attgtgaatc gtatcgtgga gacgcatggc ggggagatgt ggttcgagag cagctctggg
    10921 gaggggacga cggtgaatgt ttttctgcct attggaaaag agaattcagt gccgccggaa
    10981 ccgcgacgtc tgatcgatac gctccccccc agaagcgaac cttcggtata cgatgagttg
    11041 aaagactagc cggtgcgcgt acgactcgcg tccggaagaa cgctgcgcta ggaccgtttg
    11101 ccaatgagtt acttggtact cgctcgaaaa tggcgaccga tgaccttcga tgatctggtg
    11161 ggacaggagc atgtctcccg caccttatcg aacgccatcg ttagcggacg cattgcccat
    11221 gcgtttttgt tcacgggcgt tcgtggagta ggaaaaacga cgagcgcgcg cattctcgcc
    11281 aaatccttga actgcgaggc tggtcccact cccaaaccgt gtttgcaatg tcccgcatgc
    11341 accgaaatcg ctgccagcaa tgatatcgac gtccaggaaa tcgacggcgc gagctacaac
    11401 ggtgtggatg aagtgcgtcg gttgcaggaa agcttgccgt accgtccttc ccgcgatcgc
    11461 ttcaaagtcg tgatcgtcga cgaagtacat atgttgtctt cgaacgcgtg gaacgcgttt
    11521 ttgaagacct tggaggagcc accgcctcac gtgaagttca tctttgcgac gacggaagtg
    11581 cataaagttc cggtcacgat tttgagccga tgtcagcgtt atgacttcaa gctcatttcg
    11641 tcgtcgtcga ttgccaagca gcttcgtcac gtgctcgatc aggaagggat tgcggcggat
    11701 gatgccgcgt tgggactgat ggcgcgggag gcggcgggaa gcatgcgtga tgcgatgagt
    11761 ctgttggatc aggccatcgc atggggggga gacaagctcg tgggagagga gattgctcgt
    11821 gttcttggca ttgccgggcg cggcaagctt ctcgagttgg cccaagccat ggttcatggg
    11881 gatgctcaag ccgtgctcat gatcgttgcg cacttggcgg atcaaggttt cgatttggtt
    11941 caagtggcgc gagacacgct cgaagtcttg cgcgatcttg tcgtggtaaa agtatgcaac
    12001 tccccggatt cattgacaga tttgactccg gaggagttgg cgacgatgcg ggcattggtc
    12061 gcggacgcgc ggggcgagga tctcattcgc cttcaccatg gtttttcgac tggattcgat
    12121 caggtggtgc atgcgcctca gcagcgttcc gcattggaaa tgctcttgct tcgccttgtc
    12181 tttcgtcctc ccttgctacc cgtcgacgat ttgatttcgc gactcgacgg catcgaacgt
    12241 cgactggtag gacgcggagc ggccttgcca cgttcgggcg gcccggttgc gttacgaaac
    12301 catcgtgggg cgacggcgtt cgagccgacc gagcggaccg aaagcgtttc tgctggggat
    12361 cgagccgtga tggcggcgcg agcgcagccc tcggcagccg tcaaggaccc ggctcccctc
    12421 tctcgcgtcg cggctcatcg ggagattcat cctgtcgcgg acacggagcc ctgggtagcc
    12481 gaaaccgttg tggattcatc gccgattgac atggagattc cggttcagcc cacggtcgga
    12541 accgcacctt cggagcgttc gcaggagcag cctgtccgac gcgctaccga gccggcgccc
    12601 accgagcctg cccctgggtc ggaggccgct tggcgcgcga ttctcgagac ggttcagcag
    12661 caaaacgcag ccctcgctgc ggtgctcgaa catgcgattc ccgtggagat ctcgccccat
    12721 gccgtccatc tcgccatcga ggaaggctca ttttatgccg accgtgctcg cgacgatcag
    12781 gccgtcgacc ttctgactcg cgtggttcgg gcacgttttc acgcctccac cgaggtttcc
    12841 ttgtcgttgc tggataccgg aaaagcccgc ggtcgaacct tgttcgacat aaacaaagaa
    12901 cagcgcctcg agcaggaacg aaaggctcgc gcggagttgg aaagccataa actcgttcag
    12961 gcggccatcg aatcactcgg tgcgcgcgtg catctcatca aactcgacga atgacttgca
    13021 tcaaagccgt cgttcctggt tgattgcttt tattgcttcg tgcatatctc gcaaaccatg
    13081 cccaagacca aacggttcac accaccggtg ggcgacttcg tgcgccatgc tgcatccgtt
    13141 caaaagaaaa tggatgaggt gcgggaccaa ttgcgtgatc gtgaagtctc cgcgcaggtc
    13201 gacgacggca atgtgcgtgt gacggtcaca tgcgccgggc tgcttcgtcg tatcgaggtc
    13261 gacccctcgt tggtacaacg ggaaggattg gagatgatgc ttgacctcat cgtgacggcg
    13321 gcgaataagg cattggacga cgcggatcga ctggtggact ctgaagtcac gaaagcgacg
    13381 gggggtctgc gtattcctgg catcaacggc tgatcatcgt gcttcccgaa gaactattcc
    13441 gattgtctca gcggttctcg cgcctgcccg gcgttggtga gaaaactgct ttgcgctatg
    13501 cgatgtttct tcttcttgcg gacccggcca ttgcgcgcgg tcttggtcaa gaactcatcg
    13561 cgctgcacga taacgttacg ccctgcgaac gatgtggata cgtggcacgc cgcagcaccg
    13621 aaggaccgtc gctatgcgcc atttgctcgg atcatcggcg agacccttcg ttgctctgtg
    13681 tggtagctcg cgtccaggac ttgcttgcca tcgaacgaag tggagccatg cgaggtcgct
    13741 accacgttct tggcaaacta ttgtctccgc tcgaaggcgt tcatgcttct gacttgccga
    13801 tcgcatccat tcgttcacga attcaaaacg aagggatacg tgaggtgctt ctcgccacgc
    13861 cgccaacggt cgatggtgaa gcttctgcat tgttcattgc ccaggagatc ggggacctcg
    13921 aggtccgtat atctcgtctc gccagtggtg ttccccacgg tggagacctc gagtttgctg
    13981 accagattac cctgggtcgt gctatcgacg gtcgacatca ggtcgtccat gacttcagcg
    14041 aaaaggtagt gtcgaaatga cccgagccat cattcgaacc gaacaagcgc ccgcagccat
    14101 tggtccgtac tcccaagccg tccgtgctgg ggactttgtg ttctgtagtg gtcaaatccc
    14161 gctcgatcca gtctccggtc aggtcgtcga aggcgatatt cgggtgcaaa ccgaacaggt
    14221 gatgaacaac cttcgggccg tgttggaagc ggccggaggg gggttggaca agatcgtcaa
    14281 gtccaccatt ttcctagttg atctcggtga cttcggcgcc gtcaacgaaa cctacgcacg
    14341 atacttccaa ggcgatgccc ctgcccgagc ttgtgtggag gtaagcaagc tgccgcgcgg
    14401 tgtgcgcgtc gagatcgaag ccactgcgta tcttggagcg tgaaccctca tggcgaagaa
    14461 agaaaaaaag aagtctggca tcgcagaatc gttcgagccc agattgttgg ccgcgaccgc
    14521 tagcaatgcc ttggaaatgc tggaagaggt gggagacaaa gcccctgccc tcgtggaagc
    14581 atgggtgcgt gagtccaatg ccttggcggt gcatgccata gcgtgggcgg acgatgcccc
    14641 agccctcgcc cgaaaaaccg ctcgaagagg cctgaacatc ctcaaaagcc gcggagttcc
    14701 tcttccggaa aaaaacaaaa agcccgaacc tgccccccag ccggcaagct tcgaagcctg
    14761 gtatctcgct cccgatcaaa gtggggtcag cgtctttacg attggctctc actccgcggg
    14821 acaaaatttc gatattgtcg acgttcaaat tcatgaaaac gccggcgtca tcgacgttcg
    14881 ggttggtgag gcaactcgca gcggcatccg ctccgctttc agaaatatcg aaagctccag
    14941 ggggtatgcc cccgtacccg ttccggtcga atgggcacga tggcatatcg aacaagcgaa
    15001 aaaacgcaac gccacctccg gcttgatcat gcccctgggc tttgatacgt cggccaatct
    15061 gctgaaaccc gttccggaac aagagcctgt tcaccccatc gaacgggaaa atatttcatt
    15121 ggatgatacc gagatacaat ctcgcatctc cggttccagc acccttcaca acgatccgga
    15181 gtttcgaagt tggcttcccg aaatggagtt catcaacgaa ttgttgggga aaatcggtca
    15241 tcgaatcgga cccgatccgg gccagcagca gcaggaacaa atcacggcgg tattcaacga
    15301 agaagtcgcg gcagccacgg ataggttttt cacacccgat gtccgacaac gtttggcgga
    15361 gcgcttgcgc gatgccgcca tctccgtaca tgcccgtgcg ggccgagaac gaaccctcga
    15421 tgttcttgcc acggcggagg cagccaaacg cgccggtttg atcacctccc ctcccagcga
    15481 aattcctttc ctcaaagatt ttttcaacaa ggctctggcg ctcgttgccg cttcccaagg
    15541 gggaaaactc aatatcccca tcgcggctcc ccccacggag caacaaggtc ttgtggcgcc
    15601 cccggaagtc ttcgaagaag cagcgaaaac gcgcgccggg cgaacagact ccgacgcgga
    15661 atcgggatca gcctccgaat agccgacagc ccaaccttcc gctgccttgc ctctgttcaa
    15721 gatgcaacaa gcatcgaaac gaggacatga cgaggcgctt gcttctgcat cagcacacca
    15781 gaacggtaca tcggcccgca agttcatcga caggcgtttc ccggtcgatc ccgatccaag
    15841 tgcggcagcg aggagaaatc gcagccaggc gagttcgaag ctccggcgac attccccaaa
    15901 gaacgacttg taacgaatca ggaagatcat cggccagcgc ggccacggac atcggtttga
    15961 gcgatgggtt ttgacaatcg atgagcaaca cacagcgccc ttccggggca attccgaatt
    16021 ctcgaacgag ttcgagcatg ttgcgaagga tgacgatttg cacgtggctc gccaaagaaa
    16081 gcgacaagct gcgagcgagg tcttggcggt ggctggctac gaatacaatg ggttgcgatg
    16141 attctgccgg gggcttgctc gaatacgttc ccgccatgga aagggccgct gccgcgcccc
    16201 gcggaaagcg acccgaggaa atgggtttgg cggctggcgg aaaccatgaa ggatatcgcg
    16261 gtgttggcgc ggttcggaaa gaggcccggg gcggggtcga atgatgatgc ggaatcggtg
    16321 tcgaacgctg cgacgacgac accgccggca tccttggacg cgacgaggag cgtcggtgcg
    16381 gagcatcatg cacgaatgct gacggctgtt ggcgttgctc gagcgatgga gtcgagccga
    16441 gcagcatgtc aatcacgcgt tgcaagtcgc tgaccatggc ttcgacgggt tcggggccga
    16501 tggttcgcgc gagcgcttct cgcagcggtc cggtcacgaa gtcttggaga cggtttggat
    16561 ccgtaggcaa ttgttccgca cgaaacccct cgagcgcctg agccaaaatc ccatctcgaa
    16621 cacggggggt gacgaccgaa tcgatgatct cgcacagggc gcaagccact agatttctgg
    16681 tctcggaatg ggttcggggc ggcatgagta ctctcagcta gcgtagcact tcaatcacat
    16741 ggcatcgaga accatggtcc gcgtgtccct cagccataaa tcgcgtgcga tggtatcagg
    16801 gtgcgggttt gcccagacgt caataggaga aaacgaattg tgaatgggat tgccggaggc
    16861 ttgaccgtct gcaccggaac atcgagggga agaagcacca tcgagacata aaaatagaac
    16921 gtaaaatcaa ttggttatga aaaatcgatg gtataggcac cgtctttcat gattcgtaac
    16981 gggggcatat tttcacgaat gagctgcacc tcgagcacct tcacgcgagg ttgaattcgg
    17041 ggaatataga cacggtcgat atgcaccacc gcatcgggac ccgagaactg cttgggaccc
    17101 acgatccccc caaagtggtc gcttcgacca aaagcgatat gcaaaccgag cttttcatcc
    17161 agcaggatct caccagaagg ctcgacacca aacgcatcca ataccccgag ccccaattcc
    17221 gcaatattgg cgtacgccgg ttcctcggca attcttttgg cttcgcgacg tccctgttcc
    17281 gattgttcgg cgtcgatcac ctcgaccgcg cggttgtggg agattcgata cgtcacgata
    17341 tcaccgtcga gttcgaccgg aagcaaacct tcggactgac ttggatctgc ctttcgttct
    17401 ccttcatacg ggacgatata ggattcgccc gacggcaggt taccggccac gcctgggtct
    17461 gggaatacgc caccggaagc gtgggcggta cgatggcgaa gatctagccg aagcagcctt
    17521 tcggtcgact gatcgacgag aaagcggcag gtggcggata cggcttcatc gagcaaggat
    17581 ttgagcagga gcacccgacg attgatttct tcgtagtcga ggcgaagggc tgggatcatg
    17641 ctgggggaga agccgggcat ggtggcggcg cgaaacggaa aagtgcgagc ggcgattttt
    17701 agcggcgcgg tggcggaaaa ctccgtggga gccaccagaa gcgggtgaga acgaaatatt
    17761 tgttcgaagg aaacggcagg atggttggcc agcgattcgg cgttgggagc aagggcatcg
    17821 acttcgaggt cagggcttac gtaccatgcc ttctctggca ggtccccgtt attcgaatgc
    17881 gcgttgcggt aaagcacgca atgaacctcc aacgggaagc tttcgagctt gtgtaatccc
    17941 gccacccact gtgccaccat gcgtcgacgc tgcgcccatg gctcggaatc cgcgaccacg
    18001 gcatccggca aatcgataag aaatgcgatg gaccgttccg tagcccgagg cttgaatact
    18061 ttgtgaatca aggtcgacaa ctcttcgggc aacaa
//
LOCUS       DAOSOR010000037        18040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_3128_length_18040_cov_4.166639, whole genome shotgun sequence.
ACCESSION   DAOSOR010000037 DAOSOR010000000
VERSION     DAOSOR010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 18040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18040
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3128_length_18040_cov_4.166639"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..514)
                     /locus_tag="PK710_03505"
     CDS             complement(107..514)
                     /locus_tag="PK710_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08808.1"
                     /translation="MKTLRIRVNDKVYDVTVEVVSDDDAPVATNGAPKTAPKATEGPR
                     PAMPSSPVARSTSASAQSLDAVTAPIAGTIQKIFVQVGGHIEAKAPALLMDAMKMDTY
                     IYAPRSGTIAKVAVNPGDAVRVGQLLFEYEEEA"
     gene            complement(511..843)
                     /locus_tag="PK710_03510"
     CDS             complement(511..843)
                     /locus_tag="PK710_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HOT08809.1"
                     /translation="MTVDDLVLFTTVIGVVMLVMVLLALFIYALARLAAGLDKPVATA
                     ADVTDDELGDELSPEIVAVLAAAASEILGGRTQIRSVRLHREADAPEWSTAGRMDIML
                     SHRVGPRR"
     gene            complement(840..2510)
                     /locus_tag="PK710_03515"
     CDS             complement(840..2510)
                     /locus_tag="PK710_03515"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOT08810.1"
                     /translation="MRTRFAIFADPLIVHERTKWLEDERGIDEMNDRNERGGSRNDAA
                     QVEQPELSGIALLEHTRARIEAGGGSAAVDKQHKRGKLTARERLDRLYDPGTFLELGM
                     FAKHRCEALAGKDIPGDGVVVGRGEVHGRNVLAFAQDLTVAGGSLGERHAQKIVELCQ
                     RALRCGAPVVGFNDSGGARIQEGVHSLSGYGRVFYFNTMLSGVVPQISVVAGPCAGGA
                     AYSPALTDFVIMIDQTAHMFITGPTVIRAVTGEEITEEALGGANVHASVSGNVHLVAH
                     SEDDAIAKVKQLLSYLPSNNLQPPPELRFDEPLVDDTVFDDLLPEDPRKPYDMHLLIE
                     RIVDYGEFFEIQPTFAPNILVGFARICGMVVGIVANQPAVMAGTLDINASDKASRFIR
                     TCNIYNVPLLTLVDVPGFLPGVDQEHGGIIRHGAKMLFAYGSASVPKVTLIVRKAYGG
                     AYLAMCSKDMGADIVLAWPSAEIAVMGPEAAVRVLNRDLLNDPEALDEAIAEYRRTHA
                     NPYRAAESLHVDDVIRPAWTRRLVGQWIAANTDKRVLRPNKKHGNIPL"
     gene            complement(2865..3329)
                     /locus_tag="PK710_03520"
     CDS             complement(2865..3329)
                     /locus_tag="PK710_03520"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOT08811.1"
                     /translation="MSRVFTKRVREMRTLLRASLSSIVATGVDGVTYQAVLVLAFGSY
                     WMAAIVGALLGGLTNFTLNRTWAFTATAKDIRLQAFEYAIACLATYLALQTCLYVLIE
                     GLAISAPIAWVPAKIAAWLSVSYPMQRLFVFSTSRRLPKHDDRVSEHPPVSL"
     gene            complement(3326..4180)
                     /locus_tag="PK710_03525"
     CDS             complement(3326..4180)
                     /locus_tag="PK710_03525"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOT08812.1"
                     /translation="MTLSSLGIAHLYRYSVGKAAIHMDGTFNQSAATSLIVVISVAAF
                     FLTAVSGYAVRSLLVGRRHDEKLQRLGGTMLLGAWFMEAVYWAIRAPGKLFARLGVKP
                     DTLTIISLLLTLAAAPIAAVGHFSTAGLLFLVGSAFDALDGMVARETGAVKKSGAMLD
                     SVFDRYSDAAPMIGLAIFYRFSVWQMLLPLVALMGSQLVSYVRAKAEAMDLSLPSTLM
                     RRHERVAYISGALILAPLLSPVLGNPWGAVHPATLTIILIVALVSNLVAVQLLIAARK
                     LLDAKERG"
     gene            4753..4974
                     /locus_tag="PK710_03530"
     CDS             4753..4974
                     /locus_tag="PK710_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08813.1"
                     /translation="MDILNTELIAGITLLHAFLAVFVVLIAGIAVQALRKDKLAGQDL
                     QREATCPHCGWKGRLSKYQRKCPGCNQQV"
     gene            complement(5048..6205)
                     /locus_tag="PK710_03535"
     CDS             complement(5048..6205)
                     /locus_tag="PK710_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08814.1"
                     /translation="MAKSDRDCTQPCSAEGRCVLHQGLCVAASEQGCKRSKACEISGL
                     CALRGDRCAPGSHEDCKKTQNCQLLGACDYVDGQCVAQVDESCASSQLCSTFGRCKAS
                     AGQCVVPDAWQGAATGVCRNLPGCAHWALCSSADASCAVSREEDCRASFWCSEYGACT
                     LGQVDGKPRCIARSSADCAASGRCQAKGLCTAKKGACVAASDTDCARSLACKEDNACI
                     ARDDRCVSSCQDSAACKVDGRCSDRLGACEAKSKSECMASNVCAQFGQCSAVNGACRV
                     VSGEDCSQSVACKEHGQCSARKGGCKATIVAQCLESLGCKRDGRCSLREDGCEVRGSQ
                     DCLRSEACKRWGRCAPKDRWSCHAASNEDCKNSETCQRQGRCRASQGECVR"
     gene            complement(6474..8816)
                     /locus_tag="PK710_03540"
     CDS             complement(6474..8816)
                     /locus_tag="PK710_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08815.1"
                     /translation="MLSVPWQVTHGLWIGWTAAAAVVVLGGVLTWLWVLWLARRRAKR
                     IIHSFGEGTGVPVPGAVVLRGRLEVPGAPVPSLDGTGAAAACSVEASSERLRFVPLPT
                     STVAHFRAPSLHIRFAQGSAALRGAVSVAAGSREFLPGSLSLLDGEDRDRLLASPDDA
                     LTFRHGSWPKDLRVGGVIRTLRHGDEVIVRGVLQKSIADAGPGASYRPMATTLELVAA
                     QDTEAIEVSAIRVMDVSFGKATLLIRGLAAGIALAIGVGIAVGAAARNVDDERVAALV
                     PPYRGWAVDMLAQQAVIGPPSQNRLATVLAYHDLVPDCRRTGRALMDHGQWAHAATML
                     ASCEEEDSLRLAMAAYRVEGQFAKACEIAERVTPAATRSQAAYLLLGGCYGQAAAVLR
                     RVATETSARDDGDVTLQCLADALDQLAGKRSDSATFVAGVSEGCQIVQADALDGPARM
                     QILSSLADAPLVELKRYRYRTLLALLMAERDVYSVEFPALSVPPIIELMTQPTHATAG
                     RAHRLEYDVLMNLAALKEPRFDQRRLRRNLALRVASFEILAGHAQEAFPLIELADKDN
                     VVARREATTNMQSDAWVESIEAEAEAVGLRVLAHVMCGQVDAASRVMEPWSDTSPARF
                     VRDIPSFVDGPSALSRIQSVVVRHVDDAAVVVAVAKDKTLAGKTIADELASALFGEMP
                     TNTSAAVGSLQHLPWMVGTNRIAPQPSDRMSWVQASSRTPVDSPMLAMWQAAVAHRIA
                     TLSGVVDGMDQSKRALEAYRSAMMNRSTTIILALLWDEGA"
     gene            complement(8906..9343)
                     /locus_tag="PK710_03545"
     CDS             complement(8906..9343)
                     /locus_tag="PK710_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08816.1"
                     /translation="MASKKVNRESDEPITKAAKRPTSPKPTQKTASKESKARKSKPRD
                     DARSSESLQAASEKQDSKLTYDDALALAEALVDMVNQGAEHPIAFFISPDDALGRELM
                     QRQASNAESWDGLVPVIVDAPFVHRYAMLTVIDELLWRKSDGS"
     gene            complement(9450..10202)
                     /locus_tag="PK710_03550"
     CDS             complement(9450..10202)
                     /locus_tag="PK710_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT08817.1"
                     /translation="MNGPLGFLVLGVGDAFSTLHYASCLLVGFERQWLLIDCPHPIRK
                     MLREATEKIGFSLDIDSIGHVVLTHLHADHCSGVEGFLFFHRYAVKQKPVLVGHESVM
                     SKLWSGHLAAGMETSTDLSTGMERTRSLEEFASVVHIPTQGEVRVGPFSIACRTTMHT
                     VPTTALRIRAGHRCLGYSSDTSFDPELIDWLAEADMFVHETNAGIHTEYEKLCNLPGN
                     LREKMKIIHYPDSFRVDGAEIEPLREGGWYIV"
     gene            10337..10600
                     /locus_tag="PK710_03555"
     CDS             10337..10600
                     /locus_tag="PK710_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08818.1"
                     /translation="MKSLWIGFALLAAIVGCGDDQATKFCLGAEGTSQDCAIGCKTAK
                     NKKACDKWAKKTRELCPKVGKEKCQELCEKDENPTACELAKSM"
     gene            complement(10610..12289)
                     /locus_tag="PK710_03560"
     CDS             complement(10610..12289)
                     /locus_tag="PK710_03560"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing serine/threonine-protein
                     kinase"
                     /protein_id="HOT08819.1"
                     /translation="MALFFLVEKGPRPGLQLALQEGRPLILGRGMTVDLPLPAEDSGI
                     ALRHAEVLVSPLDVQIHPLPNVGPVFVNGSPIENATRMVAGDSLQVGDTTIRLYSDQG
                     PDSESMIEPIPLSFRDIIESARALRLDSQRRAPTPSGLASENLHCTGCGIAGIVPPRP
                     DLWDAAWLCSACAKERRKPMAGMPARLGHYELLRPIARGGMGVVFEGLDSDTGLHVAI
                     KILRSSGPRAERAAKRFAREQQITMALRHPNIVCCYDIGVWEGSLYIVSEFVPGGDAL
                     KYSSLTSPLQQVLWLGADLFRALGYGHDLGIVHRDVKPANLLLCPVAPQGTLRGKLGD
                     YGLAKSRSGVGNPITMSNESGGSLLTIGPEQIEDFVNVGPQGDLYAGAATVFWMLTGD
                     TPLVLPCASSSATFEQKAQAILHPARKRLLDLRGDVPVPVAELIDGLVAVDPSLRQRM
                     HAREVAAAFNAIAARMVDKSTWTGSAPASFEASDHARVLKRSLEAVEFCVRMLERSVE
                     RAAKEVREAADTEDNQRMQRALANHESAQADLEQALTQWEGLLDADHGPRG"
     gene            complement(12418..13296)
                     /locus_tag="PK710_03565"
     CDS             complement(12418..13296)
                     /locus_tag="PK710_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOT08820.1"
                     /translation="MLIAIPSDMPGGLDAPISEHFGHCDVFTLIHVSDGKVGEVRLIP
                     NGGHEHGGCMAPVHLLKDNGVEVLVAGGMGMRPLAGFQSVGIDVFFKEDASTVRDAVS
                     MYTEGKCRPFGKAQTCGGGAGQCGGHHGHEAKRPPIEGPADVRDGRIVTMEFAIRDEG
                     GNLIEASEPGSAMRYLQGSGGIEGLEKAIAGKQAGDEIRVTLQPSEAFGDHDEQRIIE
                     VPRSKLPQDVTLGEIVTAQHPTGAQVHMMVIDVNDESVRLDGNHPLAGKVLQFEVKIL
                     KVEAATEDEIEHGHVH"
     gene            complement(13321..15411)
                     /gene="metG"
                     /locus_tag="PK710_03570"
     CDS             complement(13321..15411)
                     /gene="metG"
                     /locus_tag="PK710_03570"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF001100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOT08821.1"
                     /translation="MTSATFERILVTAALPYANGPLHIGHLAGAYLPADIFVRFQRLR
                     GKPVLFICGSDEHGVPIVLRARKDGVSPQAIVDRFHVQMRDAFAAFGMSFDHFGRTSG
                     AVHADTSQAFFSVLDEKSSFVQKTESQLFDPKAGMFLADRFVRGTCPHCEYDDAYGDQ
                     CEKCGRSLSPKDLKNPRSVISDAIPEVRETTHWYLPLGVLQPKLEAWLSHHEDWKVNV
                     LGQVRSWLQEGLGDRAVTRDQSWGVPVPKKSAEKAGIDATGKVLYVWFDAPIGYISFT
                     AEWAQVQGQPDDWKRWWQDPGTRLVQFIGKDNIVFHCLIFPAMLQAHGGFVLPDAVPA
                     NEFLNLEGQKLSTSRGWAVWLHEFLEDFPADYLRYSLARILPETKDADFSFTDFQAHI
                     NNELADTLGNLINRSLTFAVRYFDGKIPSLEHPSEADSAMLAEIARYPAIVAEHLESF
                     HFREALSEVMALARVGNKYFNDAAPWATRKSDPRACGNTIHVAAQLCACLSILIDPFL
                     PFTAASVRRMLGIDGVSSASGTQATIHWDDAVRPLIEAGQTLGPIEILFHKVQDEQVA
                     KQRAKLDAAMPAAPAPKAAFLPMGDTITYDDFAKLDLRVGRVISCEPVKKSKKLLRCE
                     VDLGFETRQILAGVALHFKPEHLIGRNVIVVANLAPRTMMGLESHGMMLMATDREDRL
                     FALSADAEPGSVVR"
     gene            15564..17447
                     /locus_tag="PK710_03575"
     CDS             15564..17447
                     /locus_tag="PK710_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08822.1"
                     /translation="MRRTYSFLSVLVAGTMAFAGACGDSDDEPGEGGHGAATGGSGGS
                     GETGGSGGSSETGGSGGSGETEKFVHSNKIDILFMIDNSASMGDKQKILAAAVPDLVE
                     RLINPICVNPVSREFEASVGPEEDCPTGTEREFEPVVDIHIGVITSSLGGHGADSCSN
                     VPSQYWNPTTEDMAHLITRGKNDAGNSVTVDTYQGRGFLNWDPTGENNPPGENNAATL
                     KQDFTNLVRGADQTGCGYESSLEAWRRFLVDPAPYEKMVPVNCPGGDTANRCRGPEGV
                     DQTVLQQRKDFLRPDSLVAIVMLTDENDCSVIDGGQYYLALQMSDGAAFFHLARGTEA
                     CQTDPWSPDCKSCWEVNPTDHPECAEGWASPEKDDPLNLRCWDQKRRFGIDFLYPVQR
                     YVKALTDPVLENGTVNPLFCGEPSANGKSCQGGVRDRSQVVLTGIVGVPWQDLAVDPT
                     DLTKGYRTSKDIDWDLILGDPQNNVKPTDPLMMETVEPRTGSHPLTGQPLSSEKGLPN
                     AINGAERLISSHDDLQYACIFELGAFGPIDCNLPENKFGCDCNGSPENPLCWNGSSYG
                     TTQYRAKAYPGLRHLSVLKDLGSQGVVASICPSNMTELSAADYGYRPAFSALVRRLSH
                     NLR"
BASE COUNT         4167 a         5482 c         4723 g         3668 t
ORIGIN      
        1 caggatggcc gccgcaagcg gcgtgatggc cccggcgacg gcgagcatgc acgcgcctcg
       61 ccatgtcaga tgagcgaacc ccgtgctttg gagcaactcc cacacgtcat gcctcctctt
      121 cgtactcgaa caggagctga cccacacgga cggcatcacc tggattaacc gctactttgg
      181 cgatggtccc cgatcgcggc gcatagatgt acgtatccat tttcatggcg tccatcaaaa
      241 gcgcgggggc cttggcttcg atatgtcccc ccacctgaac gaatattttt tggatcgttc
      301 cagcgatggg tgccgtcact gcatcgaggc tttgggcact cgcactcgtc gagcgagcca
      361 cagggctcga cggcatagcc ggcctcggcc cttcggtggc tttcggagcc gttttcggag
      421 caccgttggt ggcaactggc gcatcgtcgt ccgacaccac ctccaccgtc acatcatata
      481 ccttgtcatt gactcgaatt cgcagcgttt tcatcgtcga ggccctacgc ggtgagaaag
      541 catgatgtcc attcggccag cggtcgacca ttcgggagcg tccgcttcgc gatgcaagcg
      601 aacactgcgg atttgcgttc gcccgccaag tatttccgaa gctgctgccg ctagcacggc
      661 aacgatttcc ggagacaact catcgcccaa ctcgtcgtcg gttacgtccg cggccgttgc
      721 cactggtttg tccaatccag ccgccagacg agccagcgca tagatgaaca atgcgagcaa
      781 caccatgacg agcatgacga cgccaatgac ggtggtgaac aacaccaaat catcaacagt
      841 catagcggga tgtttccgtg tttcttgttg ggacgaagca cgcgtttgtc cgtgttcgcc
      901 gcgatccatt gccccacgag ccttctggtc cacgcgggtc gaatgacatc atcgacgtgc
      961 aaggattcgg cggcacgata cggatttgcg tgggttcgac gatactcagc gatcgcctcg
     1021 tcgagggctt ccggatcgtt gagcaagtct cggttcaaca cacgaacggc agcctccggc
     1081 cccatcacgg cgatctctgc cgatggccat gcgagcacga tatccgcccc catgtccttg
     1141 gaacacatag ccaaataggc tccaccgtag gccttgcgaa caatgagcgt caccttggga
     1201 acggaagcag aaccataggc gaagagcatt ttcgcgccgt gacgaatgat gcccccatgt
     1261 tcctgatcga ctcccgggag gaatccaggc acatcgacaa gggtcaacaa cggaacgtta
     1321 tagatgttgc acgtgcgaat gaagcgagaa gctttgtccg acgcgttgat atcgagtgtt
     1381 cctgccatga cagcgggctg attggccaca atacccacga ccatcccgca gattcgagca
     1441 aacccaacca agatattggg ggcaaaggtt ggttggatct cgaaaaactc tccgtaatcg
     1501 acgatgcgct cgatgagcaa atgcatgtca taaggttttc ggggatcctc gggcaacagg
     1561 tcatcaaaga cagtatcgtc taccagcggc tcatcgaacc gcaattcagg cggcggctgc
     1621 aggttgttgg aaggtaagta cgagagaagc tgcttgacct tcgcgatcgc atcgtcctcc
     1681 gagtgcgcca cgagatggac gttaccggat acggaggcgt gtacgttcgc tccgccgagt
     1741 gcctcttcgg taatctcttc cccggtgaca gcacgaataa cggtcggccc agtgataaac
     1801 atgtgggctg tctgatcaat catgatgacg aagtcggtaa gagcgggcga gtaggcagca
     1861 ccccccgcgc aagggcccgc gaccacggag atctgcggta cgacacccga gagcatggtg
     1921 ttgaagtaaa acaccctccc atagccggaa agggaatgga ccccttcttg gatccgcgcg
     1981 ccgccggaat cattgaaccc gactaccggt gctccacaac gaagggcacg ctggcataat
     2041 tccacgattt tttgggcgtg ccgttctccc aaggaacctc ccgccacggt caagtcttgc
     2101 gcaaaagcca agacatttcg cccgtggact tctccccttc ccaccaccac gccatcgccc
     2161 ggaatatcct tccccgccag cgcctcacag cgatgcttgg cgaacatccc caattccaaa
     2221 aacgtccccg gatcgtaaag ccggtcgagc cgttcccgag ccgtcagctt gccgcgctta
     2281 tgctgcttgt ccacggccgc ggaccctccc cccgcttcga tccgcgcacg agtgtgctcg
     2341 agcaacgcaa tcccactcaa ttctggttgc tcaacctgag ccgcgtcgtt tcgggaaccg
     2401 cctcgttcgt tccgatcgtt catctcgtcg atgcctcgtt cgtcttccaa ccacttcgtc
     2461 cgctcatgga cgatcagcgg atctgcgaat atcgcgaacc gagtccgcac tcaccatgct
     2521 ttcccatcgg tcgcaacgga agcgtggttt catggaagct ggtgtcgtgt tgtttgcgac
     2581 ctgacgcgcg aagacaccta tcatgaacga caacaatcgc aagtccggag gagccggccg
     2641 atggcatcgt tccgtgttgc caagcttttc acgaacgctg cccgcccaaa atccccgccg
     2701 attcgatgac caaactcgct gcccgcggcg ttgtctcccg ccatgcccca ccgcgattga
     2761 ccccacagat gcttgtttcc gacaaccaag cctcagccat gttttcccag attacttatt
     2821 aaaatgtatc acgaatcatc gttcaatccg tttatgggag gcgctcaaag agaaaccgga
     2881 gggtgctcgg aaacccgatc gtcgtgcttt ggcaggcgac gcgacgtgga gaagacaaaa
     2941 aggcgttgca tcggatagga aacgctgagc caagcggcga ttttggcagg cacccaggcg
     3001 atgggagcac tgatggcaag cccttcgatc aacacataca gacaggtttg aagagcgagg
     3061 taagtagcca gacaagcgat ggcgtattcg aacgcttgca gccgaatgtc cttggcagtt
     3121 gcggtaaacg cccaggttcg attgagagtg aagttggtca atcctccaag caaagctccg
     3181 acaatggcgg ccatccaata gcttccgaaa gccaatacca acacggcttg ataggtcacc
     3241 ccatccacgc cagtagccac gatcgacgat agggaggcgc gtaacagcgt tcgcatttca
     3301 cgaactcgtt tggtgaagac tcggctcatc cacgctcctt ggcatcgagg agctttcgtg
     3361 ccgcaatcag caactgaacc gcgaccagat tggacacgag cgcgacgatg agaatgatcg
     3421 tgagggtagc gggatgcaca gcgccccagg ggtttcccaa caccggcgac aacaggggag
     3481 ccaggatgag cgccccggag atgtaggcca ctcgctcgtg acgtctcatc aatgtgctgg
     3541 gcagagaaag atccatggct tccgctttgg cgcgaacgta gctcacgagt tgcgagccca
     3601 tcaaagccac caagggcaag agcatttgcc acacggaaaa gcggtaaaaa atcgcaaggc
     3661 caatcatggg cgcggcatcg gagtatcgat cgaaaaccga atcgagcatg gcccccgatt
     3721 tttttacagc gcccgtttcg cgtgccacca tcccgtcgag ggcatcaaac gcacttccca
     3781 cgagaaacaa taaacccgcc gtggaaaaat gtcccaccgc tgcgatgggg gccgccgcca
     3841 acgtcagaag gagcgaaatg atcgtcaagg tatcgggctt gacccccagg cgcgcaaaca
     3901 attttcctgg cgcccgaatc gcccaataca cggcctccat gaaccaagcc cccaacaaca
     3961 tcgtgccccc aagccgctgc aacttctcgt cgtgcctcct tcccaccaga agtgaacgaa
     4021 cggcatatcc cgacaccgcg gtcagaaaaa acgccgcaac cgaaatcacg acgatcaacg
     4081 aagtcgccgc tgactgattg aatgttccat ccatgtgaat agcagctttt ccgactgaat
     4141 aacgataaag atgcgcaatg ccaagcgatg acaaagtcat ccttgatgag agtcaggtca
     4201 agatttttgc atctaccatg ccatcattcg tagtatcgat ccgcggtcac tttccttccc
     4261 tggaccaacg aatatctcct ttcggtgcga aattaacttc gcttccccac ccccccgcga
     4321 tgtacggctc gtgcaatcga ctcggtgctt ccaccagtcg cacaagtcga attccagcgt
     4381 atttctcgcg caacacgaag attgcccggc ccacacaccg cggggaaaga tgctcggtca
     4441 gtagttttct ccaccctacc ctatcggacc aaaggacaga gcagcgtgtt caaaaacctg
     4501 acaggctctg gctcatttgt cagacgtttc gacaggttcg cgtcgctctt gcctcagttc
     4561 atcccatcgc gtaccttcga gcccccaggg gtgccgagcc cccatcgtac cactgctggt
     4621 ttgggcagag ctttctttcc cttcgtcgtt ttcacgctga tcccccctct accgatagcg
     4681 ggtcgttcga aaagcttcgg agttattcta ttgaccagct accgttacac cagtcgttga
     4741 cgagagttgc tcatggatat cctcaacact gaattgattg cgggcatcac gctccttcat
     4801 gcgttcctgg ctgtcttcgt tgttctgata gcgggtattg cagtgcaagc cttgagaaaa
     4861 gacaagctcg caggccaaga ccttcagcgc gaggcgacat gccctcattg cggttggaaa
     4921 ggacgtctaa gcaagtatca gcgcaagtgc cccggatgca accagcaagt gtgacgcgtt
     4981 cccatcgcgt cccttggggc catcttcccg ttcccaggaa gacttcgccc ccccatcgat
     5041 gccatcgtca acgcacacac tcaccttggg atgcccggca gcgtccttgt cgttggcacg
     5101 tttcggagtt cttgcagtcc tcgttcgacg cggcgtgaca agaccatcga tccttgggag
     5161 cacaccgccc ccagcgcttg caagcctcgg atcgtaaaca gtcttgcgag ccgcgtactt
     5221 cgcacccatc ttcacgcaac gaacaacgac cgtctctttt gcatcccaac gactccaagc
     5281 actgcgccac aatggtggcc ttgcacccac cttttctcgc actgcattgc ccgtgttcct
     5341 tacaggctac ggattgacta cagtcttcgc cagacaccac gcgacaggcg ccattgaccg
     5401 cggagcactg tccgaattgt gcacaaacgt tcgaggccat acattccgac ttggacttgg
     5461 cctcacacgc tcccagacga tccgaacatc ggccgtccac cttgcatgca gccgaatcct
     5521 gacacgaaga cacacatcga tcgtccctgg cgatgcatgc attatcttct ttgcatgcta
     5581 gcgatcgcgc gcaatccgta tcgctcgccg ctacgcacgc tccttttttt gccgtgcata
     5641 atcctttcgc ctgacaacgt ccggaagcgg cacaatccgc tgacgaccga gcgatgcacc
     5701 gcggctttcc atccacttgt ccgagggtgc aggcgccgta ttccgaacac caaaacgaag
     5761 cgcggcaatc ctcttctctc gatacggcac aagaagcatc cgccgaagaa cacaacgccc
     5821 aatgagcaca tccaggtagg ttccggcata cgcccgtcgc ggcgccttgc catgcatcgg
     5881 gtacgacgca ttggcccgcg ctagccttgc atctgccaaa cgtcgaacac agttgtgagg
     5941 aagcacagga ttcatcgact tgtgccacgc attggccatc cacgtagtca catgcgccaa
     6001 gtagctgaca attctgcgtt tttttacaat cctcgtggga tccgggagca cagcgatccc
     6061 cacgcaaagc gcacaatcca gaaatctcac atgccttcga ccgtttacaa ccttgctctg
     6121 acgccgcaac gcaaaggccc tggtgaagga cgcaacgtcc ttccgccgag cacggttgcg
     6181 tacaatctcg atcggatttt gccacacaca atccattttc gacggaacac cgcgcgtgtt
     6241 gacggcaacc ttccgaagat tcacatgccg atggcgttgc tacacagccg ggccatggga
     6301 gccaacgagt agcgcatcgt ccctcatcga cgcatgaacg ctgcgtaggg caccgaagca
     6361 atgcccacac gcccgagagg acgaatgcga ccaggagccc cgtcacaaca ggtatcgcga
     6421 ttcgtatggc tcggttcaag gtcatgatgg ttcgtagtgc cattcggtcc gcctcacgct
     6481 ccttcgtccc acaaaagcgc caggatgatc gttgtggaac gattcatcat tgcggatcga
     6541 taagcctcca aggctcgttt gctctggtcc atgccatcaa caacgcccga caacgtggct
     6601 atccgatgcg caacggcggc ctgccacatg gcgagcatgg gagagtccac cggtgttcga
     6661 gaagaagctt gcacccaaga cattcgatca gaaggttgcg gagcaatacg attggttccc
     6721 accatccacg gcagatgttg cagagagcct accgctgctg acgtgtttgt cggcatctcg
     6781 ccgaaaagcg cagaggccag ctcatcggct atggtttttc cggcaagcgt tttgtctttg
     6841 gctacagcca cgacgaccgc agcatcgtcc acatgccgaa ccaccacgga ttgaattctc
     6901 gaaagagccg agggaccatc gacaaaggac gggatatcgc gaacaaaccg ggcgggagat
     6961 gtgtcagacc aaggttccat cacacgggag gcagcgtcga cttgcccaca catcacatga
     7021 gcaagaacac gtaatccaac tgcttctgcc tcggcttcga tactttccac ccacgcgtcc
     7081 gattgcatat ttgtcgtcgc ctcccgtcga gccacgacat tgtctttgtc ggcaagttca
     7141 atcagcggga aagcttcttg ggcgtggccg gcgagaatct cgaaactggc tacccgaagg
     7201 gcaaggtttc ttcgcaagcg acgctgatcg aaacgcggtt ctttgagtgc cgcaaggttc
     7261 atcagcacat cgtattccaa cctgtgggct cgacccgccg tcgcatgcgt tggttgcgtc
     7321 atcaattcga tgatgggcgg cacggatagc gcgggaaatt ccacggaata aacgtcgcgc
     7381 tcggccatca gtagcgccag caacgttcga taacggtatc gtttgagttc gaccaaaggc
     7441 gcatccgcca gagaggacag aatctgcatc ctggcagggc catcaagcgc atcagcctgg
     7501 acaatttgac aaccttcgga aacgccggca acgaaagtag cgctatccga acgctttcct
     7561 gccaactggt ccaaagcatc cgccaggcat tgcaacgtca catcgccatc atcacgcgcg
     7621 gaagtttcgg tcgcgacccg tcgtaagacc gctgcagctt gcccataaca ccctccgagt
     7681 agcaaatagg ctgcctgcga tcgagtggct gcaggcgtca cgcgctcggc aatctcgcac
     7741 gcttttgcga actgcccttc gacacgatac gcggccatgg ctagacgcaa tgagtcttcc
     7801 tcttcgcaac ttgctagcat cgttgccgcg tgagcccatt gcccatggtc catcaaggca
     7861 cgcccagtgc gtcggcaatc cggaaccaaa tcatgatatg ccagaaccgt ggccaaacga
     7921 ttctgacttg gaggaccgat gactgcttgt tgggccagca tgtccacagc ccaacctcgg
     7981 tatggcggta cgagagcagc tactcgttca tcgtccacat ttcgtgcggc tgcccccacc
     8041 gcaatcccca ccccaatcgc caaggcaatt ccggcagcaa gcccacgaat gagcaacgtg
     8101 gcctttccaa acgatacatc catcactcgt atcgctgata cttcgatggc ctccgtgtcc
     8161 tgcgcagcaa cgagttccag cgtggtggcc atgggacggt acgaggctcc ggggcccgca
     8221 tccgcaatgc ttttttgaag cactccacga acgatgacct catcaccgtg ccgcagcgtg
     8281 cgtatcactc ctcccacacg aaggtccttg ggccatgagc catggcgaaa agtcagcgca
     8341 tcatcgggcg atgccaatag ccggtcccgg tcttccccat cgagaagcga taggctcccc
     8401 ggcaagaact ctcgtgagcc ggcggccacg gacaccgctc cacgcaacgc cgcacttcct
     8461 tgtgcaaatc ggatgtgcaa cgatggggcg cgaaaatgcg ccaccgtgct cgtgggcagg
     8521 ggaacgaaac gtaatcgttc cgaagatgcc tcgacggaac aagccgcggc cgcccccgta
     8581 ccatccaagg aaggcacggg cgctcctggc acttccaaac gacctcgcaa cacaaccgcg
     8641 ccaggtacgg gcacgccggt accctcaccg aaggaatgga taatgcgctt ggcgcgtcga
     8701 cgcgccagcc acaacaccca cagccaggtc aagacaccgc ccaaaacgac gacagcggct
     8761 gcggcagtcc aaccaatcca caggccgtgg gtcacctgcc aaggtacgga cagcatgggg
     8821 tccaagcata caccctagtg ccaaccatgc gcatcttgtt gcacaacttt acaaaacgaa
     8881 ttcgcttatc gaagcgtcag gtttttcacg aaccatccga tttgcgccac aaaagctcat
     8941 cgatcacggt caacatcgca tatcgatgaa cgaaaggagc atcgacaatg acagggacca
     9001 atccatccca gctttccgca ttcgatgctt gacgctgcat gagttcgcga cccaaggcgt
     9061 cgtcagggga gatgaaaaac gcgatcggat gttcagctcc ctgattgacc atgtcgacga
     9121 gggcttctgc aagcgccaaa gcatcatcgt aggtaagctt cgagtcttgc ttttccgatg
     9181 cggcttgaag gctttccgaa gaccgagcgt catcacgagg tttggattta cgcgccttgc
     9241 tttccttcga tgcagttttt tgtgtgggct ttggcgaagt cggtcgtttc gctgccttgg
     9301 tgattggttc atccgattcg cgattcactt tttttgaggc catgacagtc tcccaagacg
     9361 aaaacgggtt ggctcgtctc acgacatcga ctccgtcatt acgaaaaagt cgtccatgga
     9421 tgaagaatcg attttcgtga gtgcgggact cacacgatgt accaccctcc ttcacgaagg
     9481 ggttcgatct ccgcaccgtc gacacgaaat gagtcggggt aatggataat cttcattttt
     9541 tctcgcaaat tcccgggcaa attgcataat ttttcatatt ccgtatggat gcccgcattc
     9601 gtctcatgga caaacatgtc ggcttctgcg agccaatcga tcagctccgg atcaaacgac
     9661 gtatccgaac tgtaaccaag acaacgatga cccgcccgaa tccgcaacgc ggtcgttggc
     9721 acggtgtgca tcgtggttcg acaagcgatg gaaaatggcc ccacacgcac ctcgccttgc
     9781 gtgggaatat gaaccaccga agcgaattct tccaaggacc tcgtgcgctc cattcccgtc
     9841 gaaaggtccg tgctggtttc cattcccgct gccaaatggc ccgaccaaag cttggacatc
     9901 acgctttcat ggcctaccag aaccggtttt tgcttcacgg cataacggtg aaagaagaga
     9961 aacccttcca cgccggagca atggtccgca tgaaggtggg taagcacgac atggcctatg
    10021 ctgtcgatgt ccaacgaaaa gccgattttc tcggtagcct ctcgcagcat ttttcgaatg
    10081 ggatgaggac aatcgatcaa caaccactgc cgttcgaacc ccacgagcag acaagaagca
    10141 tagtgaaggg tcgaaaacgc atcgccgacg ccaagtacaa gaaaccctaa aggaccattc
    10201 atcggtaggg atgacacatg gctcttgtct cattgctcgg tggttgcgtc aatcaccgag
    10261 gctcgttggc ttgcgttgtc ggcaaatcga aacggtgcgt gttgccggcc ttgtcgaagg
    10321 gtgatagcac cgaaggatga agtcgttgtg gattggcttt gcgctcctgg ccgcgatcgt
    10381 gggttgcggc gacgatcaag ccacgaagtt ctgtttgggg gcggaaggca cgagtcaaga
    10441 ctgcgccatt gggtgcaaga ccgcgaaaaa caagaaagct tgtgataagt gggcgaaaaa
    10501 aacgcgggag ctttgcccca aggtggggaa ggaaaaatgc caagaactct gcgaaaaaga
    10561 cgagaatccg acagcttgtg agttggccaa gtcgatgtga gaattccgtt cagccacgcg
    10621 gaccatggtc cgcatcaagc agtccttccc attgggtaag cgcctgctcc aagtccgctt
    10681 gcgcactttc gtggttcgcc aacgcccgtt gcatgcgttg gttatcctcg gtgtctgccg
    10741 cctcacgcac ttcttttgcc gctcgctcca cagaccgttc gagcattcga acacaaaatt
    10801 cgaccgcctc caaggatcgc ttcaacaccc tcgcatggtc ggaagcctcg aacgatgccg
    10861 gggcagaccc cgtccaagta cttttgtcga ccatccttgc cgcaatcgca ttgaacgcgg
    10921 ccgctacttc gcgcgcatgc atccgctgac gtaggctggg gtcaacggcc acgagcccat
    10981 cgatgagttc ggccacggga accggaacat caccgcgcaa gtcaagcaat cgcttccgag
    11041 caggatgaag gatggcttgt gccttttgct caaacgttgc gctcgaagat gcacacggca
    11101 gcaccaacgg cgtgtccccg gtaagcatcc aaaacacggt cgcagccccc gcatacaagt
    11161 ccccctgcgg ccctacattg acaaaatcct cgatctgttc aggaccaatg gtcaataacg
    11221 acccaccact ttcattgctc atcgtgatcg gattaccgac cccggaacgg cttttcgcga
    11281 gtccataatc accgagcttg ccgcgcaacg tcccctgcgg agctaccgga cataacaata
    11341 aatttgcggg tttcacatca cgatgcacga ttcccaggtc gtgaccgtaa ccaagcgcac
    11401 gaaatagatc ggcgcccagc cacaacactt gctgcaatgg actcgtcagc gacgaatact
    11461 tcagagcgtc acccccaggg acaaattccg atacgatata taaggaccct tcccacaccc
    11521 caatgtcgta acaacagacg atgttgggat gacgcaacgc catggtgatc tgctgctcac
    11581 gcgcaaaacg tttggccgct cgctctgccc taggtccgga ggaacgcaaa attttgatcg
    11641 ccacgtgcag acccgtgtcg ctatcgagcc cctcgaatac gacacccata ccaccacggg
    11701 caatcggacg aagcagctcg taatggccca accgcgcggg catccccgcc atgggcttgc
    11761 gacgttcttt cgcgcaagcg gagcaaagcc acgccgcatc ccataagtcg ggtcggggcg
    11821 gcacgatacc ggcgattccg catcccgtgc aatgcaagtt ctcactcgcc agcccgctgg
    11881 gggtaggcgc acgacgttgt gaatcgagcc gcagcgcccg cgcactttca atgatgtcgc
    11941 gaaacgacag cggaatgggt tcaatcatcg attcggagtc cggtccttga tcggaataca
    12001 atcggatggt cgtatctccc acttgcaacg aatcgccggc caccattcgt gttgcgtttt
    12061 caatcgggct accgttgacg aatacggggc caacattcgg caaaggatgg atttgaacgt
    12121 ccaatggcga caccaaaacc tccgcatgac gcaacgcaat accgctgtct tccgcgggaa
    12181 gaggaagatc cacagtcatg ccgcgtccaa gaatcaaggg acgaccttct tgcagagcga
    12241 gttgcaaacc gggtcgtggt cccttctcga cgaggaagaa cagagccatg gttcatttga
    12301 tgatgtttga acatgggcgc tgtcgcccgg cgggaagcga tactatggca ggattggaaa
    12361 cgatgacaag gccaaggaga aaaaaccaat agattaggca aaggttggaa agcgaaatca
    12421 gtgaacgtga ccgtgctcga tttcgtcttc cgtggcagcc tccaccttga gtatcttgac
    12481 ttcaaactgc aaaaccttgc ctgccaacgg atgattaccg tccaaacgaa cgctctcgtc
    12541 attcacatcg atgaccatca tatgaacctg cgctccggtg ggatgctgcg ccgtcacgat
    12601 ctcgccaagc gtgacatcct gcggcaattt cgaacgagga acctcgatga tgcgctgctc
    12661 gtcatgatca ccaaaagcct cggaaggctg caacgttaca cgaatctcat cgcccgcttg
    12721 cttgccggca atggcttttt ccagtccttc gattcccccc gagccttgta agtagcgcat
    12781 cgcgctgcct ggctccgaag cttcgatgag attgccgccc tcatcgcgaa tggcaaactc
    12841 catcgtgaca atccgtccgt cacgaacgtc cgcaggccct tcgataggcg gtcgcttcgc
    12901 ctcgtgacca tgatggccgc cgcattgtcc tgccccacct ccacaggtct gcgctttgcc
    12961 gaatgggcgg cacttgcctt ccgtatacat cgaaaccgca tcgcgcaccg tcgaagcgtc
    13021 ttctttgaaa aacacgtcga taccgacgct ttgaaaaccg gccagcggac gcatccccat
    13081 gccgcccgcc acaagtacct cgactccgtt gtctttcaac aaatgaacgg gagccatgca
    13141 tccgccatgc tcgtgccccc cattgggaat gagacgaacc tcgcctactt tgccgtcaga
    13201 aacatgaatc agcgtgaaca catcgcaatg accgaagtgt tcggagatgg gggcgtcgag
    13261 cccaccaggc atatcggaag ggatggcaat gagcacggta atctcctttg tggttgaggc
    13321 tcagcggacg acggagccgg gctctgcgtc agcggataga gcgaaaagac ggtcttcacg
    13381 gtctgtagcc ataagcatca ttccgtggct ttctagtccc atcatggtac gaggggcgag
    13441 attggccacc acgatgacgt ttcgcccgat caagtgctcg ggcttgaagt gcagcgctac
    13501 ccccgcgaga atctgccggg tctcgaaccc cagatccacc tcgcaacgca ggagtttttt
    13561 cgactttttt acgggttcac aggaaatcac gcgaccaacc cgaaggtcga gtttggcaaa
    13621 gtcatcgtaa gtaatcgtat cacccatggg aaggaaagcg gctttcggtg ccggggctgc
    13681 aggcatggcc gcgtcgagct tcgcgcgctg tttggccacc tgctcgtctt gcaccttgtg
    13741 aaaaaggatt tcaatgggac ccagggtttg gcctgcctcg atgagcggac gaaccgcgtc
    13801 atcccagtgt atcgtggctt gggtgccaga tgcactgctc acgccgtcga tccccagcat
    13861 gcgacgcaca ctggccgcag taaacggaag gaatggatcg atcaagatgg acaagcaggc
    13921 gcaaagttgc gcggccacgt gaatggtgtt gccgcaggct cgcgggtcag atttgcgcgt
    13981 agcccatggc gcggcatcgt tgaagtactt gtttcccacc ctcgccaacg ccatcacctc
    14041 gctcaacgcc tcgcggaaat ggaaagactc caaatgttcc gcgacgatgg ccggatagcg
    14101 cgcgatttcc gcgagcatcg ccgaatccgc ttccgaagga tgttcaagag acgggatttt
    14161 tccgtcgaaa tatcggaccg caaacgtcaa actgcggttg atcaagttgc caagggtatc
    14221 cgcaagttca ttgttgatat gggcttgaaa gtcggtgaac gaaaaatccg cgtctttggt
    14281 ttcaggaaga atgcgagcta gcgaataccg caggtaatcc gctggaaagt cttccaaaaa
    14341 ttcgtggagc cataccgccc atccacggct cgtcgatagc ttctgccctt ccaagttgag
    14401 aaattcgttg gccggtaccg cgtcgggaag cacgaatccg ccgtgcgctt ggagcatcgc
    14461 cggaaaaatc aagcagtgaa acacgatgtt atccttgcca atgaactgca cgagccgcgt
    14521 gccagggtcc tgccaccatc gcttccagtc gtccggctgc ccttgcacct gcgcccactc
    14581 cgccgtgaac gaaatatatc caatcggcgc gtcgaaccac acataaagca ccttgcccgt
    14641 cgcatcgata cccgcttttt ccgcggattt tttcggaact ggcacgcccc aggattgatc
    14701 tcgcgtcacg gcgcgatccc caagcccctc ctgcaaccaa cttctcacct gacccaacac
    14761 attgactttc caatcttcgt gatggctcaa ccaagcctcc aacttgggct gcagcacacc
    14821 cagaggcaag taccaatgcg tggtctctcg cacttccgga atcgcatccg aaatgacact
    14881 gcgtgggttt ttcaagtctt ttggactcaa cgagcgaccg catttctcac actggtcccc
    14941 gtaagcgtcg tcgtattcac aatgcgggca cgtgccccga acgaatcgat ccgccaaaaa
    15001 catcccggcc ttcggatcga aaagctgcga ttccgttttc tgcacgaacg agcttttctc
    15061 gtctaacacc gagaaaaacg cctgactcgt gtccgcgtgg accgcccccg aagtcctgcc
    15121 gaaatgatcg aaactcattc caaaagccgc aaatgcgtcg cgcatctgca cgtggaaccg
    15181 atccacgatc gcttgcggcg ataccccgtc ttttcgcgca cgaagcacaa tcggcacccc
    15241 gtgctcatcc gaaccacaga tgaacaaaac cggtttaccg cgtaaacgct ggaagcgaac
    15301 gaaaatatct gcgggcagat aagcacccgc taggtgtccg atatgaaggg ggccgttggc
    15361 gtacggcagc gccgcggtca cgagaattcg ttcaaaagtc gctgaagtca tggttcggcg
    15421 ggccaacata atgaatcgac caggatccgc aatgaccaga tctcaatctt ttcccgttca
    15481 agagggaagg atgaagggga aatttcgtac cacgaataac agttttcagg atttttcctg
    15541 caaggaactt aggagcaacg aaaatgcgtc gaacgtattc ttttctatcc gtactcgtgg
    15601 ccggaacgat ggcttttgcc ggagcatgcg gcgattccga tgatgaaccc ggggaaggag
    15661 gacacggcgc ggcaacgggc ggtagcggag gctcaggcga gacgggaggc agtggtggtt
    15721 ccagtgagac aggcggcagc ggcggttccg gtgagactga aaaatttgta catagcaaca
    15781 aaatcgatat tttgttcatg atcgacaact cggcgtctat gggggataag cagaaaattc
    15841 tcgcggcagc ggtccctgat ctcgtggaac ggctcatcaa cccgatttgc gtaaatccag
    15901 tgtcacgaga gttcgaagcg tccgttggac cagaagaaga ctgtccaacc ggaaccgaac
    15961 gtgagttcga acccgttgtc gacattcata ttggcgtcat cacctcttct cttggcggcc
    16021 acggcgcgga ttcttgcagc aacgtacctt cccagtactg gaaccccacc acggaggata
    16081 tggcccatct gatcacgcgt ggaaaaaacg atgccggcaa ttccgttacg gttgatacct
    16141 accaaggacg cggctttttg aattgggatc cgacgggtga gaacaacccg ccgggggaga
    16201 acaacgccgc cacgctgaag caagacttca cgaatttggt tcgaggagcg gaccagaccg
    16261 gctgcggtta cgaatcgagc ctcgaggcct ggcgacgatt cctcgtcgac cccgctcctt
    16321 atgagaagat ggtgcctgtc aactgcccgg ggggagatac tgccaaccgc tgtcgcggcc
    16381 ccgagggtgt cgaccagacg gttcttcaac aacgcaaaga cttcctgcgc ccggattcgt
    16441 tggtggccat tgtcatgctc acggatgaaa acgactgctc cgtcatcgac ggcggacagt
    16501 attacctggc gttacaaatg tcggacggcg ccgcgttttt ccacctcgcc cgaggcacgg
    16561 aagcgtgtca gacggacccg tggagtccgg attgcaaatc ttgttgggaa gtcaacccca
    16621 ccgatcatcc ggaatgcgcc gagggctggg ccagtcccga aaaagacgac ccgctcaacc
    16681 tgcgttgctg ggatcagaaa cgtcgcttcg gcatcgattt tctctaccct gtccagcgct
    16741 atgtcaaagc cttgacggat ccagtgctgg aaaacggcac ggtcaatcca ttgttctgtg
    16801 gggagccaag cgccaacggg aagagctgcc aaggaggcgt gcgtgaccga agccaagtcg
    16861 tgctcacggg tatcgtgggt gttccgtggc aagacctggc tgtcgacccg accgacctga
    16921 ccaagggtta tcgtacttcg aaagatattg attgggatct tatcttgggt gatccccaaa
    16981 acaacgtgaa accgaccgat ccgttgatga tggaaactgt tgagccgcga acaggttccc
    17041 atcctttgac cggtcagccc ttatcctcgg agaaggggtt gcccaacgcc atcaacggcg
    17101 cggaacggct gatttcaagt catgatgacc ttcagtacgc ttgcattttc gaactcgggg
    17161 ccttcggtcc catcgactgc aatcttccag aaaacaaatt tggttgtgat tgcaacggct
    17221 cgcccgagaa tccactttgc tggaacggca gcagctacgg tacgacgcaa tatcgcgcca
    17281 aagcctatcc gggactccgt catctttcgg tgttgaaaga cctgggctcc caaggtgtgg
    17341 tggcatcgat atgcccttcg aatatgacag agcttagcgc tgcggattac ggctatcggc
    17401 cagcgttcag cgccctcgtt cgacgtcttt cccataacct ccgatagttt tcccctattt
    17461 tccactccct cacaacaaaa acaccgggca gcggcaccgt tttggatagc gctatccgtt
    17521 cggaaattgg attgtagaat caagcttagt gctcctatga gttcgccaca cgggttcctc
    17581 gaagagcaat ggctccccat ggtatattcc gtcataggcg cctcggccga aagcgacgcc
    17641 tgggacccaa acacagccgc acatacggca aatacccctg gatagtcgta cccaatgggt
    17701 ttcaattccg acataggtat ccaaatagag gacttcatgg cgctcctgtg gatgctcacg
    17761 ccatggaacc cgcatctggc ccgcggcata gagcatgtct ccggatactc gccaccatga
    17821 aggccatccg ccgcgttgcc gatttcgtta tccgcggaac catccagcgc taccaccgcc
    17881 gtttcctgcc ataagcgacc atcgatgcaa gcggtatccg ccccaatgcc cgcgacccac
    17941 acaccacccc ccgacgaaaa ccccttcgac tcctcaaagc cccacgatag cgcccccaga
    18001 ccacgacgta ctccaaaaac aaatttgaat atcaataggt
//
LOCUS       DAOSOR010000038        17834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_3192_length_17834_cov_4.962990, whole genome shotgun sequence.
ACCESSION   DAOSOR010000038 DAOSOR010000000
VERSION     DAOSOR010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 17834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17834
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3192_length_17834_cov_4.962990"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="PK710_03580"
     CDS             <1..962
                     /locus_tag="PK710_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08823.1"
                     /translation="PLQLEPLTDLAIGAGLQWIVVASPQDLARHKVLSQSFARFIPPT
                     RRHIFHRLTGIDLDAMQRLVVAGYERSVLFLMNGMFDPRATEEVFHNRFLQNGSRKVY
                     RPDAVWSHGIVAGNEVRAIAALSPNVIAVEGGQAFRSKIALLLALGRLHRSPRAFDLP
                     DMKRVYGELGDGVVHVLAPGPFEGDWQKALGGMLGVTSVIGARVRLLDDGQMNLAIRL
                     AGSWQGDEKRAAAILLDRYGDMTNSSIGKLLHLHEPTLEPSPFERPDLVGVDVQLDGI
                     RCLGGLYDVIAADLGDMLSLGRATDRIDDASMRDSDEDLHTHG"
     gene            937..1491
                     /locus_tag="PK710_03585"
     CDS             937..1491
                     /locus_tag="PK710_03585"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOT08824.1"
                     /translation="MKIYTRTGDDGTTSLFGGKRVSKAGDRVEAYGVVDEANSILGLA
                     RAMKLDPWSDEVLQKIQRDLFTLGAEIASGRNAQSKLRMPLIGSADVQRIEKAIDEAE
                     QQLAPLHSFILPGGSLAASTLHLARTVVRRAERMLVALSNSEPVRQEILEYINRASDL
                     MFVLARLANMHQGVEDVPWLPDKG"
     gene            1527..2219
                     /locus_tag="PK710_03590"
     CDS             1527..2219
                     /locus_tag="PK710_03590"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOT08825.1"
                     /translation="MTETLRRHSKAKRNHLFRYRVKPSGLDFSVDTNFPESRRHYELR
                     TMLYQIAKLALSSQCSIGSAQPVFWNAADPSRFVTPDLFIRFESPDSLFESWYAWERG
                     APEVAVEILSDSDATELAWQNRLRNYHELGVEEVVRFDPVAPADGLRVWNRVHGDLVE
                     RDLEDPRIAESSMLGLYWVVIKNATLGRVLRLAKDNSGGRLLPTPVEREARRRREAEL
                     RVRELEQKLKNK"
     gene            complement(2222..2761)
                     /locus_tag="PK710_03595"
     CDS             complement(2222..2761)
                     /locus_tag="PK710_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT08826.1"
                     /translation="MSCMNLTAKDMGQRLALLRPELISHGLRLTKDMSQAEDLAQDTI
                     ERALRFESRFEEGTNLRAWVHHILTNLFISGCRRQRREVKAFAVLSTDPCAWTASGTD
                     RIAMEGFTRSVQRAIDDLPSRFRETLVLSDIEELSYKQVAERLRVPVGTVMSRLHRGR
                     RLLGQALQDGGECLIDRAA"
     gene            complement(3457..3969)
                     /locus_tag="PK710_03600"
     CDS             complement(3457..3969)
                     /locus_tag="PK710_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08827.1"
                     /translation="MSEKVKLNTTPSKIDVKNEAWVLHFPRFGAEGTHFHTYGQLTPY
                     FQGLTQGKLMGTHCVNPACPISKGNGELWVPPRIDCPDCHAKMVWKEIKNPQGYIYTY
                     TLVERGGTGLEIETPYYQVDVFVQGVCTIIKGYLINRTPIRIGDKVKAGFLTGDQATH
                     TCLDIYWELI"
     gene            complement(3980..5380)
                     /locus_tag="PK710_03605"
     CDS             complement(3980..5380)
                     /locus_tag="PK710_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08828.1"
                     /translation="MIEFAPGNLRVPKWSRPVYMVAAGTSDFRKRYPEKKLEELCMDA
                     FRMLLEENDLKMAPLDVKGLINMCNYGEFADHFQDQLLCEAKVHDYLGLNLLPNWGVK
                     TGGATGGYTMLSGANTVASGYSDCTLVLGWERMDEVITWTGNYYIACAACKDFETRLA
                     RNYASYYAPMARRFGEMYKVDERIRAEIAVKNRYYACMSPFAQQPGRHTVDEVLAGAI
                     VSDPLRFLECCAMSVGSSAALLCSEELAYKLTDHPVKLSICGGSDTLRTGDRRPMKIP
                     LLPHETEEDYKQLYEDMKKTDGRWPGFESFGATRFAAYMAYRMAGIQNPIEDLDLVET
                     HDAFTISDLQTYGDVGLTLYGQEQEYIRSGDCYLHNPYTNKPGKCPSNLSGGLLGTMH
                     AVGATGIWQCAEVFWQLQNKYDKFHGDPKMWERFGKKKPDDWKSLQVHNPRRGLAISH
                     AGVGSHVTCAVLEKAW"
     gene            5558..7366
                     /locus_tag="PK710_03610"
     CDS             5558..7366
                     /locus_tag="PK710_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HOT08829.1"
                     /translation="MSRETKRPRKDRTSDPSESQRMRDLEAQLIGTRRVLTAFCDIAS
                     ALAAPADADRLLGLVLDRASALLDADRATLYLIDDATGELVSKLVVGGGEQTIRLAVG
                     QGIAGAVVQTGKPIRVADAYTDDRFNRSWDGKTGYRTRSILAVPMKNHVGQTIGVLQV
                     LNKREGKPFTDEDENLLGVLAMQAAISIDNVKLYLAVVEKNNVLAQVRHTLERKVDFL
                     NLLFELESTMSRATDQRSLVQPLLGLTVAATRTQAGAVFLLDQGGTQHNLFVFKREAP
                     NTVTEYLVDHVMGAFLQAIQQKGPVSIEQVRRRSKATAEQSLMGLHARSVLAVPMLSE
                     EGDAFGALVLYDREDEAPFTEDDQELLRLVAANASTAISLLRSRESQRVSDRLSTIGR
                     LLSNVLHDLKTPMAVISGYVQLMATTESAEQRKSYSELVLKQFDHITAMQREVLAFAR
                     GERTLLVSKVYLQKFFDELRLQMEKELEGRGIELQMELRDRGTARFDQAKVMRALHNL
                     ARNALDAMTAGGLLRIEIDRDTEGLVLRVSDTGPGIPEQIRGKLFRSFVTADKANGTG
                     LGLAIVKKIAEEHGGKIDVTSSPTGSTFTLHIPQTG"
     gene            7541..9352
                     /locus_tag="PK710_03615"
     CDS             7541..9352
                     /locus_tag="PK710_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOT08830.1"
                     /translation="MLFPTEVAMYRPFPLLLVVCSSLSSSLAFAHGGQILEQLPCPVE
                     EPMGLAACDNNLFISDMATRSIVRFDPCTAQILERIPAQGFLPTGIACSQGTLFLADR
                     RQDFMARMRLGKNDHPSPIPYYERWATGLVHDGQSLWVVDARSAKLHRIDPVDGTTIE
                     SFDAPDKHPTGIAFDGRMLWVADHGKDSLYRVDPSDGKVVTILPSPGPYPSALAMHQG
                     SLWVADYQSQQLFRVSLPHDEPYLEDMPRRVRVSYEVVYRVRGTGAVTHLRAALAVPE
                     ELPGQHLLDKVTFEPQPTRMTMDRWGQPIALFEREELKAGTTFSIRWTGDFLLYRVRF
                     PIDPDRVRGATLPAQLDAYLQDDKKYDLGAQSLARLVEEVTHGKTSYYDRARAIYEHL
                     ARVIKYDRSGGWNNAATVLARGTGSCSEYTFALVGMLRRAGIPARYAGAVSERGDEAS
                     FDDVYHRWAEAYMPGYGWVPMDANAGFGETPAGKAEYFGGRSNRHVVTTRGGGASEWL
                     DWTYNNHTMYEVQGDATLEEQSLARYRPLADTNKDPVLASAPRVNVPRLTEPIVDPPA
                     KPTQSTSFSGDPWMAAIALLLAAGFGIAVGRGFRTIP"
     gene            9349..10035
                     /locus_tag="PK710_03620"
     CDS             9349..10035
                     /locus_tag="PK710_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08831.1"
                     /translation="MTDIKPFVNRESPSPRARSVSVVFSVKGLDAQQPQSPGMLDPLG
                     STCLFDRMLSVLLRVGEAEHKAVWFDDAALVERFEKTSHPSIRLLSGPPERCLRAGSP
                     DALWSTHVLLLDGRHPFLRSSTIDEAIRLIRVRSDIEGMQSCVRMQGPIYSLTGESLT
                     NGSTHLLVACPAFAVVPSNHLATGRSPGSWFPFEISKSESFRADTLFHQEMAGALIDA
                     RERRESQTAH"
     gene            complement(10029..11546)
                     /locus_tag="PK710_03625"
     CDS             complement(10029..11546)
                     /locus_tag="PK710_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08832.1"
                     /translation="MSAPLAACGSGLFVDSSQDSEQKKRFRGLSSPHIWVSTTYFGEG
                     FPYSIVNNLAEILFKEMGASLQAIGLTALFHLPWNLKFLWGPFLDHYETKRRWLLGVE
                     VALTICLVALAFFACGRQLLGIIAASFFVLALLSATHDIAVDGFYLEGLDSAGQSKFV
                     GYRAMAYKVSSLVVRGPLIILIGWVGWTGGLLTAAGLFFFLTLLHWKILPDVERPGPS
                     VTALLREILRPRVLVFGTVVAGLVLLQRKTKFFDPCIEALRGALQHAPALSQLSVPGW
                     ITLGLLGALLVTLALLGPIKRRVATSNSHYATAFIDFLARPRVGVVLAFVVLFRAGES
                     FLQKMKWPFLRDELGLSLEQYGFANGTLGVIASFAGTFLGGWLIARHGLKKWIWPFVL
                     AQNTLNLLYAWLAWAGKQGAVGLGSITAVIMTEHLGEGLGTAVFMVYLMRSCDPAHKA
                     AHMALLTALMSVSFTIAGVASGFLASWLGFCPYFIFTFLATLPGMALIHYLPHRQ"
     gene            complement(11636..13891)
                     /gene="priA"
                     /locus_tag="PK710_03630"
     CDS             complement(11636..13891)
                     /gene="priA"
                     /locus_tag="PK710_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOT08833.1"
                     /translation="MLLAQVAIPIPLERAFTYAVPDQYAPRIAPGMRCLCKFRRRLVV
                     GVIIAVEQAQAPAGIKLIALEELIDDIPSVPEELLQFLLRLSSYYLAPIGEVLRLALP
                     VLEHQRADRLRTKLPLFAEPPRAVERSVLVAYPVASTPQTPALRGAPASVLTHLRTVG
                     PTAIVRLAEEYANARSAVKRLESLGLVTVRREAQKEAPFFHDTKARESAPVPTESQKT
                     AIEHIEKALLAGIPRSFLLHGVTGSGKTEVYLRAIEACLRAERGALVLVPEIALTPQL
                     IARFRSRFGDGVAVIHSGLSRRARYQMWKSLRENKVSVAIGARSAIFAPVPRLGLVIV
                     DEEHDGSFKQEEGVRYQGRDMALLRAHLAGAVCVLGSATPSLESEGLVQQGKLTRLRL
                     PDRATRAALPAVELVDLRRTGSLPSTHSMLSLPLYRAIETALAAGEQAIVFLNRRGFA
                     PSIQCEGCGTILQCPTCSVSTTLHRAGGARLRCHYCDYEAAMPSACAACGCPRLAMVG
                     IGTERLEEVLALAFPQARVARLDRDVAPGIRSEAVIERMRRKEADILVGTQMVTKGHD
                     LPDVTVVGVINADVALSMPDFRASERTFQLLVQVAGRAGRAERPGRVLIQTRIPEHPV
                     LAHAVTHDVASFTKTELVNRKEAGYPPYYRLVLVRLDGPDLDEVIREAERLASHARVQ
                     TQVLSHGVEVLGPSPAPIEKLRGRFRFRILLRAVQRPALRAVVAAIQQVREHRSSRVR
                     VVLDVDPMHML"
     gene            complement(14196..15410)
                     /locus_tag="PK710_03635"
     CDS             complement(14196..15410)
                     /locus_tag="PK710_03635"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT08834.1"
                     /translation="MTSAPALTQLAAPRTFGRYQLFDRIGAGGMAEIYLARATTELGG
                     ARLTVVKQIRPELSGNEKFAEMLTVEAKLAARLNHANIVQVFDLGRHENVLFIAMEYV
                     EGFDLNELLRRCARAHIPLPVDFALLIIMEALRGLSYAHRLVDDRGEAIGLVHRDVSP
                     SNVLISFEGEVKLCDFGIARANDMADLLSDDVIKGKAGYMSPEQAFGEPLDARADLFA
                     AGIVLWELLAGRKLYRAPKENEKPTLLDQARAAVIPDLPSRGLPDESVLNGIVRRALA
                     RNRDDRYASAQQMLRDLEQYVGQARLMASPIRFGEWLRENFEENVIALRRSRERAAKA
                     LQQGPAATVEPYGTPPPPPTAEPQPSAMEPAPVVEQTPNSSPQPRSNLALYIALGVFI
                     GFVIVGVLWALT"
     gene            15525..15860
                     /locus_tag="PK710_03640"
     CDS             15525..15860
                     /locus_tag="PK710_03640"
                     /inference="COORDINATES: protein motif:HMM:NF023740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3634 family protein"
                     /protein_id="HOT08835.1"
                     /translation="MTTAVILGLAVLALGIMLVMNRNATLLFSAKVQRGRIVSLRGRA
                     PKKLVRELNDVLGRRPVPSASLRVYAQGDRAALQASGDLNENEMQRLRNVLGTFPTAR
                     IRSEPFRKQ"
     gene            16205..17476
                     /locus_tag="PK710_03645"
     CDS             16205..17476
                     /locus_tag="PK710_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08836.1"
                     /translation="MKRRSTASGPLSENPSSSRSMTPPPVSASVRPTSSAPKSRSFFD
                     VPQDRDTVDERPSSVPPATESRDSKRPDAQPSSSVPPATESRDSKRPASEPPASEAPT
                     STRQQKASTEPADDTKHKPTRLEQKDPGPQPQTEGLSEAKSKDTKKKVAKSKGEEDAP
                     AKDRKRQSESKDDTVQDDRLHDDFFREGEEVEKENMALAHAGPVHFEDTGQIRLAKLR
                     TSKEVIDRRRKMQRIVGYILAPAALVAALAGVKMYKNGGNPAKNISPGTTVSMIPRAS
                     ATQPVPTADVSSVSVRQDAATDSDSARDAHESSLDAGPTDAPLEAQAVADASVVQDAA
                     PESAIAPALPDGVDARKEALKALEQGKWARAEQLAQAAIDADPEDATPYLYGATALQE
                     MGKRAEAKAVFQKCAQVAKRGPIHECRMFSK"
     gene            17492..>17834
                     /locus_tag="PK710_03650"
     CDS             17492..>17834
                     /locus_tag="PK710_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08837.1"
                     /translation="MTEPLILALILTLLAGLSTGLGGLIVFTFRRSNPSFLSLGLGFS
                     AGVMVYISFVELLTGAKASLASHYGGGVGGWMAVGGFFAGMALIATIDKLVPEPENPH
                     DAFLQQDAKGPP"
BASE COUNT         4015 a         5080 c         4998 g         3741 t
ORIGIN      
        1 aacctttgca actcgagcct ttgaccgacc tggccatcgg agctggattg caatggattg
       61 tggtggcgtc tcctcaagac cttgcaagac acaaagtatt gtcgcagtcg ttcgcgcgat
      121 tcattccacc gactcggcgc cacatctttc atcggctcac cggaatcgat ctcgatgcca
      181 tgcaacggtt ggtggtggcg ggatacgaac gatccgtatt gtttttgatg aatggaatgt
      241 tcgatccgcg agccaccgaa gaggtatttc acaaccgatt ccttcaaaat ggttcccgca
      301 aagtgtatcg tccggacgcc gtttggagtc acggaatcgt tgcaggcaac gaagttcgag
      361 cgatcgcggc cttgtcaccc aacgtcatcg cggtggaggg aggacaggct tttcgttcca
      421 agatcgcgtt gttgcttgcc ctcggcaggc tgcatcgttc tccgagagcg ttcgacttac
      481 ccgacatgaa gcgtgtgtat ggtgagcttg gcgatggggt cgtgcatgtc ctggcccccg
      541 gacctttcga aggggattgg caaaaggcat tgggtgggat gctaggggta acctcggtga
      601 ttggggcgcg ggtccgcttg ctggatgacg ggcaaatgaa tcttgcgatt cgtctggcgg
      661 gttcatggca gggcgatgaa aaacgagcag cggccatctt gctcgatcgc tatggggaca
      721 tgaccaacag ttcgatagga aaactccttc atctgcatga gccgaccctc gaaccttcgc
      781 cgttcgaacg tcctgacttg gtgggcgtgg acgttcagct cgacggtatt cgatgtctcg
      841 gaggcttgta cgacgtgatt gccgcggact tgggagatat gttgagcctg ggccgcgcga
      901 cggaccgtat cgacgatgca tccatgagag atagcgatga agatctacac acgcacgggt
      961 gatgatggga ccacgagctt atttggtggc aaaagggttt ccaaggcggg tgatcgtgta
     1021 gaggcttatg gggtggtgga tgaagccaat tcgattcttg gcctagcgcg agcgatgaag
     1081 ttggatccat ggagcgatga agttttgcaa aaaatacagc gtgacttgtt cacgttgggc
     1141 gccgagatcg caagcggccg caatgcgcag agcaagctcc gcatgccgct gattggatct
     1201 gctgacgtgc agcgtatcga aaaagcgatc gacgaagccg agcagcagtt ggcgcctttg
     1261 cactcattca tcttgcctgg cggttctctt gccgcctcca ccctgcatct ggctcgaacc
     1321 gtagttcgtc gcgccgaacg gatgcttgtc gctctttcca actctgaacc cgttcgtcaa
     1381 gagattcttg aatacatcaa tcgtgcaagc gaccttatgt ttgtcctcgc tcggctagcc
     1441 aacatgcatc agggcgtcga agacgttcct tggctacccg acaaagggtg agcgtcggca
     1501 agcaaggttc atggtaagct aacgccgtga ccgaaacgct gcgcagacat tcgaaggcca
     1561 agcggaatca tctattccgt taccgtgtga aaccaagtgg cttggacttc tctgtcgata
     1621 cgaatttccc agagtccaga cggcattacg agttgcggac gatgctatat caaatcgcga
     1681 aattagcatt gtcttcgcag tgttccatcg gctcggcgca gcccgtgttt tggaacgcgg
     1741 ccgatccgtc tcgttttgtc acgccggacc tgttcattcg tttcgaatcc cccgattcct
     1801 tattcgaatc gtggtacgcg tgggagcgag gggcacccga agtggcggtc gaaatcctga
     1861 gtgattcgga tgcaaccgaa ctcgcctggc aaaaccggct tcgtaactat catgagcttg
     1921 gcgtcgaaga agtcgtccgc ttcgacccgg ttgcccccgc cgacggtttg cgcgtgtgga
     1981 atcgcgttca tggcgacttg gtcgaacggg acctcgaaga cccaaggatc gctgaatcgt
     2041 cgatgctcgg actatattgg gtggtgatca aaaacgcgac gctcggacgt gtgttacgcc
     2101 tcgcaaaaga caatagtgga gggcggttgc tgcccacgcc ggtagaacgg gaagcccgac
     2161 gtcgtaggga agcggaactg cgggtgcgtg aactcgagca aaagctcaaa aacaaatgac
     2221 atcaagctgc tcgatcgatc aaacactcgc ccccatcctg caaggcttgc cctaacaagc
     2281 gccgaccacg atgaagacgt gacatcaccg tgccgaccgg aacacgcaac cgttccgcga
     2341 cttgcttgta cgacagctct tcaatatccg acaaaaccaa ggtttcgcga aatcgactag
     2401 gaagatcgtc gatcgcccgt tgcaccgacc gcgtaaaacc ttccatggcg attcgatccg
     2461 tgccgctggc agtccacgcg caaggatccg tcgatagaac agcgaaagct ttgacttctc
     2521 ggcgttggcg acggcatccc gaaatgaata aattcgtaag gatatggtgg acccaagcac
     2581 gaagattcgt gccctcttcg aaccgacttt cgaatcgaag ggcccgctcg atggtgtcct
     2641 gggctaggtc ctctgcctgg ctcatgtctt tcgtcaaccg taagccatga gagatgagtt
     2701 ctggtcgaag aagcgcgagt cgttgcccca tgtccttggc cgtaagattc atgcaagaca
     2761 tgacaagcaa accgaaggcc aattataata tcgttatatt tctaatagtt acatctgtgt
     2821 ggggtgtggc tggacagacg caacccgtgt ccttcatgcc tttgcccagg gcagcggtcg
     2881 gcaccgccag gatgaagggc aatgacttgg tcataccgtg gtccttttaa aaaccgtccg
     2941 aaatcatgtc taagcccgga tcggcatcag catgctttgt cagggcgtcg ataacacata
     3001 tacggatact caatacccga tcccgtcttc tggtcacgaa ttgtatacta ttcaataata
     3061 tcagcgagca gaggaggaag atattctacg atctatcctg cttcggatag ggttttcgat
     3121 tcttgaaatc gaaggcatct ccaacgatgc cgtgttgtcc gtcatggtca aaggggacag
     3181 accatcgacc gaaggcgtcg ggtcgtggct cgtacacgtt tgatgcggac acttgcaacg
     3241 tttccgtcga tgcgtattgc gggaggcgag tcgcaacatt ttcttggttg cttgcgtatg
     3301 gatgtgtcgc gaggatcgac aaggtggcga aggagcagaa gacggctcga ttcattgaaa
     3361 acgccatgag tcaggatgcg cgcggatgcg tgcaacgggg cggccacgcg caggcgcctg
     3421 gcaagcgtgt gaggaaaggc gaaatggaag gatgggttag atgagttccc agtaaatgtc
     3481 gaggcaggta tgggtggctt ggtcgccggt cagaaatcct gctttgactt tgtcaccgat
     3541 gcggatggga gtgcgattga tgagatagcc tttgatgatg gtgcatacgc cctgcacgaa
     3601 tacgtcgact tggtaatacg gggtttcgat ttcgagtccg gtacctcctc gttcgacgag
     3661 cgtataggtg tagatatagc cttgtgggtt tttgatttcc ttccacacca ttttggcgtg
     3721 gcaatcgggg caatcgatac ggggcgggac ccacaattcg ccgtttcctt tggagatggg
     3781 gcaggcagga ttgacgcaat gggtacccat caatttgccc tgggtgagtc cttggaagta
     3841 gggcgtgagt tggccgtagg tatggaagtg ggttccttcg gctccgaagc gaggaaagtg
     3901 cagcacccaa gcttcatttt tgacgtcgat cttggacgga gtggtgttca gcttgacctt
     3961 ttcgctcatg gtggacctcc taccaagcct tttcgagtac ggcgcatgtc acgtgacttc
     4021 ccacgcccgc gtgggaaatc gcgaggcccc gtcgtggatt gtggacctgc aagctcttcc
     4081 aatcatcggg ctttttcttt ccgaagcgtt cccacatttt cgggtcgcca tggaacttgt
     4141 cgtatttgtt ttgcaattgc cagaacacct cggcgcattg ccaaatgccg gtagcgccga
     4201 ccgcatgcat ggtgcctagc aatccgcccg acaggttcga ggggcacttg cctggcttgt
     4261 tggtgtaggg attgtgcagg taacaatcac cggatcgaat gtattcctgt tcctgtccgt
     4321 ataaggtcaa tccgacatcg ccgtaggttt gaagatcgga aatggtgaac gcatcgtggg
     4381 tttccaccaa gtccaagtct tcgatggggt tttggatacc ggccatgcga taggccatat
     4441 aggcggcgaa gcgtgtggcg ccgaagcttt cgaaccccgg ccatcggcca tcggtctttt
     4501 tcatgtcttc ataaagttgc ttgtaatcct cctcggtttc gtgaggaagc aagggaattt
     4561 tcatcggtcg tcgatccccc gtgcgcaggg tatccgagcc accgcatatg gatagtttga
     4621 cggggtgatc ggtcagttta taggcgagtt cttcggagca gagcagagcc gcggaggatc
     4681 cgacgctcat ggcgcagcat tcgaggaacc ggagaggatc ggagacgatg gcacccgcga
     4741 gcacttcgtc gacggtatgt cgtccgggtt gttgggcgaa gggcgacatg cacgcgtaat
     4801 agcgattttt tacggcgatt tcggcgcgaa tgcgttcatc gaccttgtac atctcgccga
     4861 atcttcgcgc catgggagcg taataactgg cgtaattgcg ggccaaacgg gtttcgaagt
     4921 ctttgcaggc ggcgcaggcg atgtagtagt tgcctgtcca ggtaatgact tcgtccatgc
     4981 gttcccaacc gagcacgagg gtgcaatcgc tatatccaga ggccacggta ttggcgccgg
     5041 acagcatggt gtagccaccg gtagcaccac cggttttgac accccagttg ggtaaaaggt
     5101 tgagcccgag gtagtcgtga accttggcct cacacaggag ttggtcctgg aagtgatcgg
     5161 cgaattcgcc gtagttgcac atattgatga gccccttgac atcgaggggg gccattttta
     5221 ggtcgttttc ctccaaaagc atccggaagg catccatgca cagctcttcg agcttttttt
     5281 ctggatagcg tttgcggaaa tccgatgtgc ccgcagcaac catgtacacc gggcgagacc
     5341 acttcggaac tctgaggttt cctggcgcaa actcaatcat tctgccctcc tgagctggaa
     5401 atgaaacgcg tcgcggaccg tagcccacgg ccaacgaacc gttacataga agaacggagc
     5461 cagcgaggct atgggaatcg tgcgaaaggc tcttcgtttc cgcgttggct catcgcccgg
     5521 tggacctagc tacctgggtc atgcttgaat cgccctcgtg agtcgcgaga caaaacggcc
     5581 gcgcaaagac cgaacgtcgg atccctccga gtcccagcga atgcgggacc tggaggcgca
     5641 gctcatcggt acgcgacggg ttttgacagc gttttgtgat atcgcaagcg ctctcgccgc
     5701 gcccgcggat gccgatcgtc tgctcggctt ggtcctcgac cgcgcttcgg ccttgctcga
     5761 tgccgaccga gccacgctct acctcatcga tgacgccacc ggcgaactcg tgagcaaact
     5821 cgtggtgggc gggggtgagc agacgatccg tctcgccgtg ggacaaggaa tcgctggtgc
     5881 cgtagtgcaa accggaaaac ccattcgggt cgcggatgcc tacacggatg atcgattcaa
     5941 ccgatcgtgg gatggaaaaa ccggttatcg aacccgctcc attcttgcag tacccatgaa
     6001 aaaccacgtg ggacagacga tcggcgtgct gcaggttctc aacaaacgcg aggggaaacc
     6061 gtttacggat gaggatgaaa acttgctcgg cgtgttggcc atgcaagccg cgatctcgat
     6121 cgacaacgtg aaactatatc tcgccgtcgt cgaaaaaaat aacgtgctgg cacaagttcg
     6181 gcacacgctc gaacgcaaag tggattttct gaatctgctt ttcgaactcg aaagcacgat
     6241 gtcacgcgcc accgatcaac gaagcctcgt gcaacctctg cttggattga ccgtggcagc
     6301 cacacgaacc caagcgggcg ccgtattcct gctcgatcag ggaggtacac aacacaatct
     6361 tttcgtgttc aagcgagagg cgcccaacac cgtaacggaa tacctcgtcg accacgtcat
     6421 gggcgcattt ctccaagcga tccaacaaaa aggtcccgtg agcatcgaac aagtgcgtcg
     6481 tcgatccaaa gccaccgccg aacaatctct gatgggactg cacgcccgat cggtgttggc
     6541 cgtgcccatg ctcagcgaag agggcgacgc cttcggagcg ttggttttgt atgaccggga
     6601 agacgaggct cccttcaccg aagatgatca ggaactgttg cgtctggtgg ctgccaatgc
     6661 gtccacggcg atcagcttgt tgcggtcgcg cgaatctcag cgtgtcagcg atcgattgtc
     6721 gacgattgga cgattgttgt cgaatgtgct gcatgacttg aagacgccga tggcggtcat
     6781 cagtggctac gtgcaactga tggccacgac ggaaagcgcc gagcagcgaa agagttattc
     6841 ggagctggtt ctcaagcaat tcgatcacat tacggccatg cagcgggaag tgctggcgtt
     6901 tgcacgcgga gaaagaacgc tgttggtcag caaggtgtat ttgcaaaaat ttttcgatga
     6961 gcttcgtttg cagatggaaa aagaactcga gggaaggggg atcgaactgc aaatggagct
     7021 tcgggatcgg ggaacggcaa ggttcgatca ggcgaaagtg atgcgagcgt tgcataacct
     7081 cgcccgcaac gccctcgatg ccatgaccgc cggaggccta ttgcgcatcg agatcgatcg
     7141 agacaccgaa gggctggtgc tgcgcgtaag cgatacgggg cctggcattc ccgagcaaat
     7201 acgcggcaaa ctatttcgct ccttcgtgac cgccgacaaa gccaatggca caggcctagg
     7261 cctggccatc gtaaaaaaaa ttgccgaaga acatggcgga aaaatcgatg tcacgtcgtc
     7321 gccgactggc tcgaccttca cgctgcatat cccccaaaca ggatgaatcc cgtggggttc
     7381 acaacgagat gaacagggtt tcccgcctgt aattttcaat atacgtatac ggatatatta
     7441 tcataattat tgaataaata caaatggtta cacgatacaa tccatattga ttatccgtat
     7501 atatagctca cgttcttgcc acccatgact cctccacggc atgctctttc ccaccgaggt
     7561 tgccatgtac cgcccctttc ccctgctcct agtggtttgc tcgtcgctca gctcttccct
     7621 cgccttcgcc cacggcggac aaatcctcga acaactcccc tgccccgtcg aagaacccat
     7681 gggcctcgcc gcctgcgaca acaacctgtt catctccgac atggcgactc gatcgatcgt
     7741 tcgattcgat ccttgcaccg cacaaattct cgaacgcatc cccgcccaag gttttttgcc
     7801 cacgggaatc gcttgctctc agggcacctt atttctagcc gatcgacgac aggacttcat
     7861 ggcccgcatg cgccttggaa aaaacgacca cccctctccc atcccgtact atgagcgatg
     7921 ggccacgggc ctggtccacg atggtcaatc cttatgggtc gtcgatgctc gtagcgccaa
     7981 gctgcatcgc atcgatccgg tggatggaac cacgatcgaa tcgttcgatg cgccggacaa
     8041 acaccccacg ggcattgcgt tcgacggtcg aatgctatgg gtggcggatc atggaaaaga
     8101 cagtttgtac cgagtcgacc cttccgacgg aaaggtcgta accattttgc catctccagg
     8161 cccttatcca tcggcgcttg cgatgcatca gggaagtctt tgggtggctg attaccaaag
     8221 ccaacagcta ttccgcgttt ctcttccgca cgatgagccg tacctcgaag acatgcctcg
     8281 tcgcgttcgg gtcagttatg aagtcgttta tcgcgtacgc ggaaccggtg ccgtcacgca
     8341 tcttcgagca gctttggcgg tgccggaaga acttccgggt cagcatttgc tcgacaaggt
     8401 tacgttcgaa ccgcagccca cacgaatgac catggatcga tggggccaac ccattgcact
     8461 cttcgaacgc gaggagttga aggcaggaac gaccttctcg attcgatgga ccggcgactt
     8521 cctattgtat cgcgttcgct ttccgatcga tccggaccgt gttcgtggcg ccaccttacc
     8581 tgcccaactc gatgcctatt tgcaggatga caaaaaatat gaccttggcg cccagtccct
     8641 cgcgcggctg gtcgaggagg taacccatgg aaaaacctcg tattatgacc gagcccgcgc
     8701 catctatgag cacctagctc gcgtcatcaa gtacgaccgt tccggaggat ggaacaatgc
     8761 cgcgaccgtg ctcgcccgag gaacgggaag ttgtagcgaa tataccttcg ctctcgtggg
     8821 tatgcttcga cgcgcgggca ttcccgctcg gtacgcgggt gctgtctccg aacgtggtga
     8881 cgaagcgagc ttcgatgatg tctatcaccg ttgggcagaa gcttatatgc ccggctatgg
     8941 gtgggtgccg atggatgcga acgcgggatt tggggagacg cccgcgggaa aagccgagta
     9001 cttcggcggc agaagcaacc gtcatgtggt gaccacccga ggaggcggcg cgagcgaatg
     9061 gttggattgg acatacaaca atcacacgat gtacgaagtg cagggcgatg ccaccctcga
     9121 ggagcaaagt ctcgcccgtt atcgcccttt ggctgatacg aacaaagatc cggttctcgc
     9181 ttcggcgccg cgcgtgaacg tcccgcgatt gaccgagccc atcgtcgacc cccccgccaa
     9241 accaacgcaa tctacatcgt tttctggcga tccgtggatg gccgccatcg ccttgctgct
     9301 tgccgcggga ttcggtatcg ctgtcggcag aggttttcga acgattccat gacagacatc
     9361 aaacccttcg tgaaccgtga atcgccatcg cctcgagcgc gttccgtatc ggttgtgttc
     9421 tcggtcaagg ggctcgacgc acaacaacca caaagccccg gtatgctcga tcccctcggt
     9481 tcgacgtgcc tattcgatcg gatgctgtcg gtgttgctcc gtgtgggcga ggccgagcac
     9541 aaggcggttt ggttcgatga tgcggcgctc gtcgaacgat tcgaaaagac atcccaccca
     9601 tccattcgtc ttctttccgg tccccccgaa cgatgcttac gtgcaggaag cccagatgcc
     9661 ctttggtcga cccatgtttt gttgctcgac ggacgccacc ctttccttcg atccagcacc
     9721 atcgatgaag cgattcggtt gattcgcgtc cgctcggaca tcgaggggat gcaatcttgt
     9781 gttcgcatgc agggaccgat ttattctctc accggcgaat cgctgacgaa cggctcgact
     9841 catctgctcg tggcgtgtcc agcgtttgcg gtcgtaccct cgaatcatct tgcgacgggt
     9901 cgttcgcctg gctcgtggtt tccgttcgag atctcgaaat ccgagtcatt tcgcgcagat
     9961 acgcttttcc accaggagat ggcgggggcg ttgatcgatg cacgggaacg gcgtgaatcc
    10021 cagaccgctc attgacgatg gggcaggtaa tgaatcaacg ccatgccggg aagggtcgcg
    10081 aggaaggtga agatgaaata gggacaaaac cctagccaac tcgccaagaa tccgcttgcc
    10141 acaccggcga tcgtaaaact tacactcatc aacgcggtca acagagccat gtgggccgct
    10201 ttatgggcgg gatcgcagct tcgcatcaga tagaccatga acaccgcggt tcccagacct
    10261 tctccaaggt gctcggtcat gatcaccgcc gtgatcgagc ccaatccgac cgccccttgc
    10321 tttcccgccc aagccagcca agcgtaaagc aaattcaacg tgttttgagc caacacgaaa
    10381 ggccatatcc actttttcaa tccatgccgc gcaatcagcc aacctccaag gaaagtaccc
    10441 gcaaacgagg cgatgacgcc aagcgtaccg ttggcgaaac cgtattgttc cagcgaaagc
    10501 cccaattcat cccgcaaaaa tggccacttc attttctgaa ggaaagattc ccccgctcga
    10561 aacaacacca cgaacgcaag caccacgccc acccgcggac gtgcaagaaa atcgatgaat
    10621 gccgtcgcat aatgcgaatt tgaagtggcc acgcggcgct tgattggacc cagcaaagcg
    10681 agcgtgacca gcaaagcccc tagcaaacca agggttatcc atcctggaac agagagttgg
    10741 gaaagcgcgg gagcatgctg caacgcgccc cgcagcgcct cgatgcatgg atcaaaaaac
    10801 ttggtcttgc gttgaagcag aacgagcccg gcaaccaccg ttccgaaaac gagcactcgc
    10861 gggcgtagga tttcgcgcaa caacgccgtg accgatgggc cgggacgttc tacatcggga
    10921 aggattttcc aatgcaaaag ggtcagaaaa aaaaacagtc ccgccgcggt gagcagtccc
    10981 cccgtccatc cgacccaacc gatgagaatg atgagcggtc ctcgaaccac caggctcgaa
    11041 accttgtagg ccatcgcccg gtatcccacg aacttcgatt gccccgctga atcgagtcct
    11101 tccaagtaaa acccatcgac agcgatgtca tgggtggcgg acaacaacgc aagcacaaaa
    11161 aagcttgccg cgatgatgcc aagcagttgt cggccacaag caaaaaacgc gagcgccacc
    11221 aaacagatgg tcagcgctac ttccacaccg agcagccatc gccgcttggt ttcataatga
    11281 tccaaaaacg gtccccaaag gaatttgagg ttccacggaa ggtgaaataa cgccgtcaat
    11341 ccaatcgctt ggaggctcgc ccccatttct ttgaacagga tttccgccag gttattgacg
    11401 atgctgtagg gaaaaccttc tccaaaatac gtcgtagata cccaaatatg ggggctggac
    11461 aaccctcgga aacgtttttt ctgttcggag tcttggctcg aatccacgaa aagaccgcta
    11521 ccacaagcag ccaaaggagc tgacatttcc agcgaatttc atggttccgc aaccgctccc
    11581 tcccttggac acgaaccacg gggaaacaca cacgaaaatt ccgttcggtg ttgcgtcaaa
    11641 gcatgtgcat cggatcgaca tcgagaacga cgcgcacccg ggaggagcga tgttctcgaa
    11701 cttgttggat agcggcgacg accgctcgca acgccggtcg ctgcacggca cgcaacagaa
    11761 tccgaaaacg gaatcttccc cgaagtttct cgatgggagc aggcgatgga ccaagcactt
    11821 cgaccccatg gctcagcact tgggtctgga cgcgagcatg gctcgccagt cgttcggcct
    11881 cccgaatcac ttcgtcaagg tctggcccat cgagtcgaac gagcaccagg cgataatacg
    11941 gtggatagcc tgcttctttg cggttcacca attccgtttt cgtgaaggaa gccacgtcgt
    12001 gggtaaccgc gtgggcaagc acgggatgct ctggaatgcg ggtttgaatg aggactcgcc
    12061 ctgggcgttc ggcccggccc gctcgacccg ccacttgcac caaaagctga aacgtccgct
    12121 ccgaagctcg aaaatcaggc atcgacaacg ctacatccgc gttgatcacc cccaccaccg
    12181 tgacgtctgg caagtcatgc cctttcgtca ccatctgggt tcccaccaaa atatccgctt
    12241 ctttgcgtcg catccgctcg atcaccgctt cactgcgaat tcccggcgcc acatcccggt
    12301 cgagccgcgc gacccgcgct tgcggaaaag ccagggcgag tacctcttcc agcctttcgg
    12361 taccaatccc caccatggca agacgcggac aaccacaggc ggcgcaggca gacggcatcg
    12421 cggcctcgta gtcacaataa tgacaacgaa gacgcgctcc ccccgcgcga tgcaaggtcg
    12481 tagataccga gcaggtggga cattgtagta tcgtgccaca accttcgcat tgaatgctcg
    12541 gcgcaaagcc ccgccgattg agaaaaacaa tggcctgctc gccagccgcc aacgccgtct
    12601 ctatggcgcg atacaacggc aaagacagca tggaatgcgt cgatggcagc gatcccgtcc
    12661 gtcgtagatc caccaactcg accgcgggta aagccgcacg agtggcccga tctggcagtc
    12721 gaagccgtgt cagcttgccc tgttgcacca aaccttccga ctcgagactc ggcgtggccg
    12781 atcccaaaac acataccgcc cccgccagat gcgcccgcaa caacgccatg tcccggcctt
    12841 ggtagcgaac cccttcttct tgcttgaaag aaccgtcgtg ctcttcgtcg acgatcacca
    12901 accccaagcg aggtacgggg gcaaaaatcg ccgagcgcgc gccgatcgcc accgacacct
    12961 tgttttccct cagcgatttc cacatttggt atcgggcccg acgactcaat ccgctatgaa
    13021 tcaccgcaac cccatcgcca aaacgggatc gaaaacgagc gatgagctgg ggagtcaacg
    13081 cgatttcggg cacgaggacc aacgcccccc gctcggcccg cagacacgcc tcgatcgctc
    13141 gtaaatacac ctccgtcttg cccgaacccg tcacgccgtg aagcaaaaat gaccgtggga
    13201 ttccagccag gagcgccttt tcgatatgtt cgatcgcggt tttctgcgac tcggtaggca
    13261 ccggcgccga ctcccgggcc ttcgtgtcat gaaaaaatgg cgcctctttt tgcgcctccc
    13321 gtcgaaccgt caccaatccc aacgactcca atcgcttcac cgccgaccgt gcattggcat
    13381 actcttccgc caatcgcacg atggccgtgg gccccaccgt tcgcaaatgg gtcaagacgg
    13441 aagcaggagc cccacgaaga gccggagtct gcggtgtgct cgccacggga tacgccacca
    13501 acaccgatcg ctccacagca cgaggcggct cagcaaataa cggaagtttc gtccgaagtc
    13561 ggtccgcccg ctgatgctcc aataccggca acgccaaccg caacacctcc ccgatcggcg
    13621 ccaggtagta cgaagaaaga cgcaacaaaa actgtagcaa ctcctccggc accgaaggga
    13681 tgtcgtcgat caattcctcc aaggcaatca gcttgattcc cgcgggagcc tgcgcctgct
    13741 ccaccgcgat gatgaccccc accacgaggc gacgacgaaa tttgcacaag cagcgcatac
    13801 cgggagcaat ccgcggggca tactggtcgg gcaccgcgta ggtaaaggcg cgttcgagag
    13861 gaatggggat ggcaacttgt gcgagcagca catcccactg ctagcacatc ctgctttcgg
    13921 caccgtgctg gatgaacaac ccacctgtcg aaggggacaa gccataccgc gaacgagccg
    13981 cgacagcaga gggggataat tccaaattat catataaatt catacagtta tatactctct
    14041 ggagagacaa acggcccggc gtcacgcggc caggctgcga taggccattg cgcttgatgc
    14101 gtggcgacaa taggcgatgg catagcctca agagtggtat ggacctgcca acgagctgat
    14161 taagcagtaa attattttgg atactgcaat gaatttcagg tcagagccca taggacgccg
    14221 acgatgacga agccgatgaa aaccccgagg gcgatgtaca gcgccaggtt ggaacgaggt
    14281 tgtggagagg agttcggggt ctgttcgacg acgggggcgg gttccatggc ggaaggttgt
    14341 ggctcggcgg tggggggggg cggaggggtt ccgtagggtt cgacggtagc ggcggggcct
    14401 tgttgtaggg cttttgcagc gcgttcgcgg gagcgtcgca gggcgatcac attttcttcg
    14461 aagttttcgc gaagccattc gccgaatcgg atggggctag ccatcaggcg ggcttgtccc
    14521 acatattgtt cgaggtcccg aagcatttgt tgcgcggatg cgtagcggtc gtcgcgattg
    14581 cgtgcgaggg cgcgtcgtac gataccgttc agaacggatt cgtcgggaag tccgcggctg
    14641 ggcaggtcgg ggatgacagc ggcacgggct tgatcgagca gtgtgggctt ttcattttct
    14701 ttgggcgcac ggtacaattt tctccccgca agcagttccc acagcacaat tcccgcggca
    14761 aaaaggtcgg ctcgcgcgtc gaggggctcg ccaaaggctt gctctgggct catatagccc
    14821 gcttttcctt tgatgacatc atccgacaac aggtccgcca tgtcattggc ccgtgcgata
    14881 ccaaagtcgc atagcttgac ctccccttcg aagctgatga gaacgttgga aggagaaaca
    14941 tcgcgatgca cgagaccgat ggcctcgccc cgatcatcga ccagccgatg cgcatagctc
    15001 aagccacgca aggcttccat gatgatgagc aaagcaaagt cgaccggcag tgggatatgc
    15061 gctcgcgcgc aacgtcgtaa caattcgttg agatcgaagc cttcgacata ttccatcgcg
    15121 atgaaaagaa cgttttcatg acgtcctagg tcgaagactt gcacaatgtt ggcgtggttg
    15181 agtcgagcgg cgagcttggc ttcgaccgtc agcatttcgg caaatttttc attgccggat
    15241 agttcgggac ggatttgctt gacaacggtc aggcgtgcgc cgccgagttc cgtggtggct
    15301 cttgccagat aaatctcggc cattcccccg gcgccaatac gatcaaagag ttgatagcga
    15361 ccgaacgtgc ggggtgcagc aagctgggtg agggcaggag cgctagtcaa ttctaacctg
    15421 ccttgtggca gcgcaaggcc atcgggtcaa gcacctgggc catctgtttg atgcctcgtc
    15481 attcgaaagc tgaacaatcc cgtccattct ggcaaaatgg gcccgtgacg acggcagtga
    15541 tcctcggttt ggcagtcttg gcgttgggaa tcatgctcgt gatgaaccgc aacgccactc
    15601 tgttgttttc tgcaaaagtg caaagaggga ggatcgtttc tctccgaggt cgcgccccta
    15661 aaaaactcgt gcgggaattg aatgatgttc tcggacgacg tcctgtgcca agcgcttcct
    15721 tgcgggtgta cgcccaaggc gatcgcgctg cactacaagc ttccggcgac ttgaatgaaa
    15781 acgagatgca gcgtcttcga aatgtgcttg ggacattccc cacggcccgg attcgctcgg
    15841 aaccattccg aaaacaataa caaccacgaa aatccgcgga tccttgtctt ataaccactt
    15901 gaatttacac atagttttct agtattctcc aagacacaac ggtgccgtcc aacggtgttc
    15961 tcacggtttt gcgacggtcg tttttcgtct tgttcagacg gtatcggccc attcgaacat
    16021 ggttcttccc cggggctggc ccatcgatac gatcttgcga agttgtgtaa ggtccagcat
    16081 aagaacgtgg tacacgtttc tcgcaaaaga acgcctgacg tgcctatcca gtgcgctgct
    16141 ggtcgggctc atcaaatcag atcccagcga ttaggagcaa gcgtttatgg ccaagaagcg
    16201 ctccatgaag cgccgatcga cagcttctgg tcctctatcc gagaacccct ccagctcccg
    16261 gtcgatgact cctccccccg tatcagcctc ggtacggcca acgagttcgg cgcctaagtc
    16321 acgctcgttt ttcgatgttc cccaagatag ggataccgtg gatgagcggc cttcgagcgt
    16381 gccgcctgcc accgaatcac gcgacagcaa acgacccgac gctcagccgt cttcgagcgt
    16441 gccgcctgcc accgaatctc gcgacagcaa acgacctgct agcgaaccgc ctgccagcga
    16501 agccccaacg tcaacgcggc agcaaaaggc gtcgaccgaa ccagccgacg atacgaagca
    16561 caagccaacc cgtttggaac aaaaggaccc agggccgcag ccacagacag agggtctttc
    16621 ggaagccaaa tcgaaggaca cgaaaaagaa ggtggcgaag tccaaaggcg aggaagacgc
    16681 cccagcgaag gatcgcaaac ggcagtcgga atccaaggac gacactgttc aagatgatcg
    16741 attgcacgac gattttttcc gcgaaggaga agaggttgaa aaagagaata tggctctcgc
    16801 ccacgctggg ccagttcact tcgaggatac cggacaaatt cgtctcgcca agctgagaac
    16861 ctccaaagaa gtcatcgatc gccgacgaaa aatgcaacga atcgtcggat acatccttgc
    16921 tcccgccgcg ctcgtggctg ccttggcagg tgtaaaaatg tataaaaatg gcggtaatcc
    16981 cgccaaaaac atcagtccgg ggaccaccgt gtccatgata cctcgcgctt cggcaaccca
    17041 gcctgtgccc acagcagatg tctcttcggt ttccgtacga caagacgccg ccaccgattc
    17101 cgattccgca cgagacgccc acgaatcgtc gctcgacgct ggaccgaccg atgcgccgct
    17161 cgaagcacaa gcagtggctg atgcatccgt cgttcaagat gccgcaccag aatcggccat
    17221 cgcaccggcg ctacccgatg gtgtcgatgc gcgcaaagag gcgctcaaag ctctggagca
    17281 aggcaagtgg gcccgtgccg agcaattggc acaagccgcg atcgacgccg atcccgagga
    17341 tgcgactccg tatttgtatg gtgcgacggc cttgcaggag atgggaaagc gcgccgaagc
    17401 aaaggctgtc tttcagaagt gtgcgcaggt cgccaaacgt ggtcccatcc acgaatgccg
    17461 tatgttttcc aaatagccgt tcgaatcccc catgacggag cctctgatcc tcgctctgat
    17521 tctgaccctc ctcgctgggt tatccaccgg gttgggcggg ctcatcgtct ttacgttccg
    17581 ccgatccaat ccgtcttttt tatctctcgg ccttggattt tcagccggag tcatggtcta
    17641 catctcgttt gtcgagttgc tgaccggtgc aaaggcttcg ttggcttcgc attatggcgg
    17701 cggcgtcggc gggtggatgg cggtaggtgg cttttttgcc ggcatggccc tgattgccac
    17761 catcgataaa ctcgtacccg agcccgaaaa cccccacgac gctttcttgc aacaagacgc
    17821 gaaaggcccc ccat
//
LOCUS       DAOSOR010000039        17614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_3240_length_17614_cov_4.536249, whole genome shotgun sequence.
ACCESSION   DAOSOR010000039 DAOSOR010000000
VERSION     DAOSOR010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 17614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17614
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3240_length_17614_cov_4.536249"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PK710_03655"
     CDS             <1..268
                     /locus_tag="PK710_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOT08838.1"
                     /translation="VSELARMGANIEVRGNAAIVQGVAALSGASVMATDLRASASLVI
                     AGLVASSHETDVLRVYHLDRGYEHIEKKLAGLGADIERISGVRA"
     gene            complement(324..683)
                     /locus_tag="PK710_03660"
     CDS             complement(324..683)
                     /locus_tag="PK710_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08839.1"
                     /translation="MTPERTLQHLEELLARLGVVVRSESFDPHVFGDASARGGLCRLH
                     DQTIVFVDARATLPDRIAVLAAAAARLDTDSVFMPPAVRQIIDGHRSRTVPWPQRPSL
                     RLIHPHKRGVTTRKKRS"
     gene            980..2731
                     /locus_tag="PK710_03665"
     CDS             980..2731
                     /locus_tag="PK710_03665"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HOT08840.1"
                     /translation="MTFTEAAAEVLRQVGRPLHYKEITAYAIEKSLLSHVGKSPEVTM
                     GARLAALFKKPTDENPMVRVRPGVFALKSWEESGLLKDMVAKAKPTARTVTAPATPEP
                     MVEPEPPPVAPAQEKKQEKPVETQPLSPPQPIRRRTKSVEAKPAAIPETATVSEPPVT
                     QETATAAPAQEAQSPVSPTVAEKSEPPPRAVIDAQPDKPPAPQRETIADDDAQAASTE
                     NEETQRADATVGTPLAVEDDDDDDQPVFSNHDADRDSSRRRRRRRRRPKGPETNGIAY
                     SAMPAGTGSPEVVAESRALPTATAGPHPVVLEVGTTSLDHVALDDFGGKDMADAIALA
                     LSAADRALGPVSLRQLAETAQRRGRLSGDLQQLQSQIAASVRADNLRRAASGQRPRFR
                     FVAGRIALTDWLLNSDLSRLEQEAIAAVERYREAARKAFVRKLQELPGHAFVELVLLV
                     FERLGFHHIRAIRRAGIPGQESHFTALHDTGVGQLPSALVIRRDGREIGRERVAEFRG
                     AFHHFGSADAGWLVTAGSVLSGAREEAAASNAATVALYDGSGIARLCEQHDIAVMRAT
                     LPICIPDLDVLEALRSS"
     gene            2728..3150
                     /locus_tag="PK710_03670"
     CDS             2728..3150
                     /locus_tag="PK710_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOT08841.1"
                     /translation="MTGPIIGVGALIVRADGHVLIARRGHPPGCGSWTLPGGKVRKNE
                     KLHDALRREILEETRLSIMVHDLLEVVEIMREGYHYVVLDYLCTPIGGENAQAGDDIT
                     AVQWIEPELETMRSLALTSDVLGIVERGLARWAQLSTR"
     gene            3206..4084
                     /locus_tag="PK710_03675"
     CDS             3206..4084
                     /locus_tag="PK710_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08842.1"
                     /translation="MASLKRWSYLLLLGSLGCSKAPPPSQFPTAQAALARMKAVYACT
                     RGVQGDGKLDHMNEAGRIRGDVMLLAVDPARVRFDVVSPFGVTLATLTSDGQRFSFFD
                     MKNSAFLEGPPEPCNIARLTQVRMPAHALVQLLRGEAPILVHKPEAATIQWNGSGYYR
                     VQIPSRHEAMQELHLEPNPSDYALPYQQQRVRVLYVRVTQRDYVHFEASLFDHADAVT
                     MQARVDELGIDPPIPPSGPACRAEIPRRIQVQVPYTHDDMRFRYEEVGLNPPLPDDIF
                     TQPIPGGVRRQYVQCR"
     gene            4126..5703
                     /locus_tag="PK710_03680"
     CDS             4126..5703
                     /locus_tag="PK710_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT08843.1"
                     /translation="MAEGSTEPSRLFCMHCGGTHPPGLRRCSSTGRSLGGDPRLIGQF
                     IARRYRIVRVLGDGPFGASYKAEHVAVGRFVSLRILHAGLVTSPDVLHRFFREARLVG
                     SVTHPRLQPVVDAGLSDDGIAYVAYQYERGRSLARAFAHNTVFSLEQAATLTSEILHG
                     LEAIHNSGFVHRALIPDSVLLQRGASGTEHAMLTNFGAATFENREENSPVSTVGLPPF
                     SRGPYLLAPHAYCLKPDRREDLFAAGVLLSAMLCPGGIPRFGGDLIALGVPAALEALI
                     ARATHPRIDARYSSASEMLAQLRSFSELVHDDVASVTETVINDLRALRNRERVLDVIP
                     ARLRLGSATSMMASALAAPLVRALKKESAQRWTEIVHRVPGIDKVLDLTADIGPIAAT
                     PIMAALEEADALCGVDDRLFCVIVGERAGKEELNEWFRTMLDAPTPECLFDGLSRLWP
                     SMVGQGIARVRQVGRGYGRFEIRDQLEPSFAICTALAGVIKAGLEHLGARSIEVSKTA
                     CEAVGDPACVFNVTWLS"
     gene            complement(5770..6810)
                     /locus_tag="PK710_03685"
     CDS             complement(5770..6810)
                     /locus_tag="PK710_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOT08844.1"
                     /translation="MKAALVGYAQTGKTTLFNALTGQAATTGAGRQDKPNLGVIKVPD
                     PRVDKLSAIYKPKKTVYAELRFVDVPGPRTKGGGLDGPTLQSLREAEALAVVLRGFEG
                     SDGSEPNPVRELDDFEAELLINDQIVVEKRLERLHKEHALGIETETLEACLAWLSEGR
                     PLRTMPLRVEQEPVIASYGFVSRRPMLAVLNVPEEKSTQQPPSGLVQAGAARSVEVMS
                     VCAAIEAEIAALPVHEQGEFLDSLGLSEPASARFIRHAYALLDYISFFTVGPDEVRAW
                     TTRRGDRAPHAAGRVHSDMERGFIRAEVMKLDEFFALGSEQRVKEQGKLRVEGKNYVV
                     EDGDILNFRFNV"
     gene            complement(6921..8243)
                     /gene="trmFO"
                     /locus_tag="PK710_03690"
     CDS             complement(6921..8243)
                     /gene="trmFO"
                     /locus_tag="PK710_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOT08845.1"
                     /translation="MNIVTVVGAGLAGSECAWQLAEQGFAVRLVEMRPARTTAAHQTG
                     LFAELVCSNSLRSGDVANAVGTLKQELLACDSLIVRLAHQHRVPAGGAVAVDRLSFAQ
                     AVTHAICSHPRIEVVQEHLDAIPKARPCVLATGPLTSDALASDLSRVLGSDNLHYYDA
                     IAPIVAADSIDWDIVFKQSRYDRSDRQSEEDAQAYVNCPLDRTQYETFVSALQQADRV
                     RPHAFEQSQFFEGCLPIEEMAQRGPMTLAFGPMKPVGLMNPRTHERPYAVVQLRLENR
                     EKTAYNIVGFQTRLTRPEQNRVFRMIPGLENAEFLRWGAIHRNTFVDAPAVLDETLQL
                     RALDGVFLAGQITGVEGYVESCACGLLCARLLVDRLNGRIPCLPPETTTMGGLLRYLS
                     QSRKPFQPSNITWAHVPPLEGRFKKHEKKAALAARALRDIEVWRDAQR"
     gene            complement(8260..9123)
                     /locus_tag="PK710_03695"
     CDS             complement(8260..9123)
                     /locus_tag="PK710_03695"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOT08846.1"
                     /translation="MAPDDPFVLTPLHPDYPIAVRAMAKAPARLFVRGTLPQARAVAI
                     VGTRRATAPALRFTTLLAQRLCEAGWSVWSGGAAGIDTAAHEGALRANGKSVVVMGTG
                     FDHLYPVCNTALFHQLLETGGAWLSLYPPSQVGTRWSFLARNELLASLVDHVVLVQAP
                     ARSGARSTVAAARRMGKTIWAVPASPWENVAEGCLLEIAAGARVLSSPTQLLGRAMKR
                     LSPCRVLSNHEQAVVDTLSSSANNLDAICELTGLSAGQVSAAVLTLTLDGLLLESADG
                     FYHLARQQQQR"
     gene            complement(9176..9784)
                     /locus_tag="PK710_03700"
     CDS             complement(9176..9784)
                     /locus_tag="PK710_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08847.1"
                     /translation="MNQRNALAIAAVLAIVTVPLACRKEPESPQPFVPTTTAPTDTQP
                     QTTAPTTTQTAPTASATSTQPSPTIFDPAVQQLLAAQLVPIAQTDAKGMKKEGEIISG
                     VLQEGQSLEQIAMLQPGKCYTVVGLGMPMVQELDIVMAPLIPLPGAPNLPMAQDLKTG
                     AQATMAPAPDCYKHVLPLPIQVKIIVTAKTGSGPVGAQLYVK"
     gene            complement(9923..10681)
                     /locus_tag="PK710_03705"
     CDS             complement(9923..10681)
                     /locus_tag="PK710_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08848.1"
                     /translation="MSKRVLLSLAAVSVIAALPLAVTSCKKDEPNTPNNFEGQYGYGQ
                     PGYGQPGYGQPGYGQPGYGQPGYTQPGQPGYTDPNQPGTQPTQPTGTTTQPAANDPFG
                     QLTGMVGAVLGGIGGGTGGDGTNAADPIAIGIRNNASQNAPGMNPDGSMVKLSLQQGQ
                     TSEGQVTLQPGKCYTLVGASSIGVLETEIKVTMPAPLTQQVLGQNAAGMNPMPVVWGG
                     GNCYRSPSPIAMPVRLEVTMKSGAGTIGIQAYSK"
     gene            10898..11881
                     /locus_tag="PK710_03710"
     CDS             10898..11881
                     /locus_tag="PK710_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08849.1"
                     /translation="MHKVNERRAKRVVVATHGHCFDGAASAAVFTRFLQSFCAEPLEF
                     SYVACGYSPQSRCDPNVLLTGDINAILDYRYTPHPNLHWYFDHHPTAFSTDEDRAHFL
                     QHQNHRRFHDTSYGSCAKLVFDIAAQPFGIDLPELAPLVSWADRIDTATFESAEQAIA
                     REHPVLQLMTVLEHAGHSTTLNQLIPRLAANPIEQVASEPAVRRSAKRFLRYHREFVK
                     AVRRNCLSFDTVVLADLSSELHEVAGKFVTYALFPRSVYSVMLTRGRHHTKISVGYNP
                     WSPFPRRHHIGMICTRYGGGGHATVGAIAHPASCVDQAKTVARQIVAELAS"
     gene            complement(11886..12287)
                     /locus_tag="PK710_03715"
     CDS             complement(11886..12287)
                     /locus_tag="PK710_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08850.1"
                     /translation="MRNETIPMVRSSSAPASAQQLALLDGLGVGDELEGYEVVWIGAV
                     RADGGMEIRLEAGGQRMRLVIALISDTPRPPVRTEKYGLYYEGLDVTHAAGEEESLRV
                     LEALAKRVRNVEAKVPTPAGLGQLRPPSTPL"
     gene            12444..13379
                     /locus_tag="PK710_03720"
     CDS             12444..13379
                     /locus_tag="PK710_03720"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT08851.1"
                     /translation="MFSNQESAEPSAQQTLENAKKRLGQIVDNKYKLISVIGIGGMGI
                     VYESEHQFLGRHVAIKILHPRYADRLEEYQRFCREARAVGALSHRTIVQVFDAGFLDG
                     TTPYFVMERLIGENLEQTLRRNKMLTARRAFWIANEVLRGLAVAHSKNILHCDMKPAN
                     IYVVRQNDRRESIKLLDFGVAKLAVEGSAASVDERTGLVFGTPQYMAPEQVTGAELDP
                     RTDIYAVGTILYESLMGEPAFQGSTREKVFRNVLQGKPKALIPPTGRLAPALQQLVSS
                     MMAKNPANRPSSAKEVSRFILAQGVIDSPNDHDYR"
     gene            13586..13942
                     /locus_tag="PK710_03725"
     CDS             13586..13942
                     /locus_tag="PK710_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOT08852.1"
                     /translation="MSLVPVAWADGNFADQEKEVLEALLEAFEANPEEAEELRTYAAT
                     PKTVDDIPVSELSFDDRRVLLQHAVLLSFADGDPSETEHKVIDAMVEKLRIPQDEASA
                     LRQTATERAKRYLNMR"
     gene            complement(13970..14347)
                     /locus_tag="PK710_03730"
     CDS             complement(13970..14347)
                     /locus_tag="PK710_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT08853.1"
                     /translation="MKILVVEDQDSIRRMIEALVTGRGHDVVGVANGAKALEVCMHDR
                     PDIVLLDLNLPGHYDGFEVCRRLRMDPSTRDVPIVIISALDDLASREKAANAGATAYY
                     TKPFSPIALLKEIETLKVQIGKN"
     gene            complement(14406..15791)
                     /locus_tag="PK710_03735"
     CDS             complement(14406..15791)
                     /locus_tag="PK710_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08854.1"
                     /translation="MSQGDRLTKQELSWLLTQEARAAAKTLRKGVARLSHSPPEITIE
                     EAPTEIESSLDALDDAMRMLDSLNASMSKRGSRGRVDLAALIVEIIPSARVRLEPGSG
                     TEVFGDEGDLRRMVQILVGQTSLLAGEVQAPEVTISREGDEVRVSVALGPETLSGDRT
                     ERAWLGRMAIRYGGRLELEGGQESLILPAEDLHDRKEMEALRKELHAAQQQGEAYARE
                     LAAVFAAGDSAEYASVAPSTVPPPQDALSPIIAFASGVASQLKTTLASLSKEIENASV
                     KGRSAESRFLEATQTCYAQLHELLSDVTRVMQVKPDEFPALVDVRPIVHNLVEHASVR
                     ADRHGVQLQSSLADKLDAVVPPSATETIIRLLLDHGIAATPRGGVVHLEACIEQRHLV
                     FVVDDGGASVPSGIRDALIWLRIDPSTVGRPRGVHLLSLYSILSSVRGNLEIADSPEG
                     GTRVLARIPAP"
     gene            16146..17552
                     /locus_tag="PK710_03740"
     CDS             16146..17552
                     /locus_tag="PK710_03740"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HOT08855.1"
                     /translation="MLNDHVFKGSGVIPSVLTGKLSDFAGMIVAPVVLCALFRIRSRP
                     GTIVAYAAVGILFSALNTIPALADAFTHWLALIGIPFRIWSDPSDLVALPALAIAYAL
                     FGSRPSTAPAWWIPKVNTAVAAIACVASSPWPSVSPATVAQNQVLMEHHAGVVFVLDA
                     PSGTMVRSFRASITDRLPPAIHDRILYQVQPQGRITGIHIDNPQEPFEVWKHPEQSAR
                     IRIRAVDAFRLYVAGPEDRLYAIDRVFGSLRWTLPIDTCRLQDLTITDDTMIVTDGPR
                     CRSVDARTGRVLWEYLAPNTVGAATIHREHVYIASSDGILWGLDVSSGRQRWRYDGGA
                     DACDSYHPSVFAQDNSVFACFDGQTRAISVQDRSVTWKQQGQPVAIAPSLLLLQSSND
                     TLHGVLTTTGKIAWTTRFDESVSARPVIWQSLAFVRDDEGRLFALDLRSGTKRWNFLW
                     PDDATSSASGTSLVVTTP"
BASE COUNT         3675 a         5053 c         5094 g         3792 t
ORIGIN      
        1 cgtgtcggag ctggctcgta tgggggccaa catcgaggtt cgcggcaatg cagcgatcgt
       61 gcagggggtt gccgccttga gtggagccag cgtcatggca acggatttgc gcgcgagtgc
      121 ttcgctcgtg atcgctggtc tcgtggcatc gtcgcatgag acggacgtgc tgcgtgtgta
      181 tcatttggat cggggctacg agcatattga aaagaagttg gcgggacttg gtgcggacat
      241 cgagcggata tcgggtgtgc gtgcatgagt cgtgtaggga agctactgcg catgaaatgg
      301 atgggcgcga ggcatgggtg gcgtcatgat cgtttctttc tggtcgtaac gccgcgtttg
      361 tgcggatgaa tgagccgcag ggaaggacgt tggggccatg gcaccgttcg gctgcgatgc
      421 ccatcgatga tttgtcgtac ggcgggtggc atgaaaaccg agtcggtatc gaggcgagcg
      481 gcggcggcgg cgagcaccgc gatgcggtcg ggcaaagtcg cacgagcatc gacgaaaacg
      541 atggtttgat catgcaatcg gcacaaccct cctcgcgcgg aggcatcgcc gaaaacgtgg
      601 gggtcgaaag attctgaacg aacgacgaca ccgagtctgg cgagcaactc ttcgaggtgt
      661 tggagtgttc gttccggagt catgccggat gctgcgaaaa agttcgaaag gtcagtacaa
      721 agcagtgtag cgcacaacgg atcggacgag ggggtggagg gcggcgatgg cgcgtcaatg
      781 aaaaaaatca aatcacggtt tgacgaatac gaaagccggt ggtatggggc ttgctttgag
      841 cggtatggtc tgagagggag tgatgaggct tcccgggcga tatcgcaagc ggtcgggggg
      901 atggcgcgag tcccgatcgt tttccctgga tggattggtg cttagattgc gcctacctag
      961 aacatctgga caggttgtca tgacgttcac tgaagccgca gcggaggtgc tgcgccaggt
     1021 tggtcggccg ttgcattaca aggaaatcac ggcctatgcg attgaaaaga gccttctgag
     1081 tcacgtggga aagagtcccg aggtgacgat gggagcgcgt ttggcggctt tattcaaaaa
     1141 gccgacagat gaaaatccca tggtgcgggt tcgtcctggc gtatttgcgc tcaaatcttg
     1201 ggaagaatca gggctgctca aagatatggt agccaaagcc aaaccgacgg cgcggacggt
     1261 tacggcgccg gctacgcccg agccgatggt ggagccagag ccgccgccag ttgccccggc
     1321 tcaagaaaaa aagcaggaaa aaccggtaga aacccagccc ctttctcccc ctcaacccat
     1381 tcgtcgccga accaaaagcg tggaagccaa accggctgcc atccctgaga cggccacggt
     1441 ttcggagccc ccggtgacgc aagaaacagc gacggcagcg ccggcccaag aagcgcaatc
     1501 gcctgtttcc cccactgttg cagaaaaatc ggagcctccg ccgcgagccg tgattgacgc
     1561 acaaccagac aaaccgccag cgccccaacg cgaaacaatc gctgacgatg atgcacaagc
     1621 ggcgagcacg gagaacgaag aaacgcaacg ggccgacgca accgtgggta ctcccctcgc
     1681 cgtagaagac gacgacgatg acgatcagcc ggttttctcc aaccacgacg ccgatcggga
     1741 cagctctcgc cgtcgacggc gccgacggcg tcgtcccaaa ggaccggaaa ccaacggcat
     1801 agcgtattcc gcgatgcccg cgggaaccgg cagcccggaa gttgtcgcgg agagccgtgc
     1861 tttgccaaca gccaccgcgg ggccccatcc ggtcgtgctg gaggtgggaa ccacgagttt
     1921 ggatcatgtg gcgttggacg attttggcgg caaggacatg gctgacgcca ttgcgttggc
     1981 tctttcggcg gccgatcgcg ccttgggtcc cgtatcgctc cgacaactcg cggaaaccgc
     2041 acagcgtcgc ggacgccttt ccggggattt gcagcaactc caatcccaaa tcgccgcttc
     2101 ggttcgagcc gacaatcttc gacgagccgc ctcgggccag cgaccgcgat tccgtttcgt
     2161 ggccggccgc attgccctga cagactggct tctcaacagc gacctgagcc gcctggaaca
     2221 agaggccatc gccgcggtcg agcgctaccg cgaggcagcc cgaaaagctt tcgtccgcaa
     2281 attgcaagaa ctgcccgggc acgctttcgt cgagttggtc ctgctggtat tcgaaaggct
     2341 cggtttccat cacatccgag cgattcgacg cgccggaatc cccgggcaag aatcgcattt
     2401 caccgccttg cacgacacag gtgtcggaca actcccctcc gccctcgtca tccgtcgcga
     2461 tggccgagaa atcggacgcg agcgtgtcgc ggaattccgc ggagccttcc accatttcgg
     2521 ctctgccgac gccggttggc tcgtgaccgc tggttccgta ctatccggtg cccgcgagga
     2581 agctgccgct tccaacgccg ccactgtcgc cctttacgat ggctccggaa tcgcacgcct
     2641 ttgcgaacaa cacgatatcg ccgtcatgcg cgccacctta cccatctgca tcccagacct
     2701 cgacgttctc gaggcccttc gttcttcgtg accggtccca tcatcggtgt cggcgcgctc
     2761 atcgttcgtg ccgatgggca tgttctcatc gcacgacggg gtcatccgcc cggttgtggc
     2821 tcatggactc ttcctggtgg aaaagttcgt aaaaacgaga agctccatga cgctcttcgc
     2881 cgtgagatcc tcgaggaaac gcggctatcg atcatggttc acgacctgct cgaggtcgtg
     2941 gagatcatga gggagggcta tcattatgtg gtgctcgact acttatgcac gccgatcggt
     3001 ggcgagaatg cccaggcggg cgacgacatc accgcggttc agtggatcga gccggaactg
     3061 gaaacgatgc ggtcgttggc gttgacgtcg gacgtattgg gtatcgtgga acgtgggttg
     3121 gcgcgttggg ctcagttgtc gacacgatga gtttcctacg cgcgggtggt ttcttctgga
     3181 aagctatact aggctacgag ttgtcatggc atcgttgaaa cgatggtcgt atttgctgtt
     3241 gctaggctcg ctggggtgtt ccaaggcacc gccgccatcg cagtttccga cagcacaagc
     3301 cgcgcttgcg cgaatgaaag cggtatatgc ttgcacgcgg ggcgtgcagg gagatggcaa
     3361 gctcgatcat atgaacgagg ccggacgcat tcgtggtgat gtgatgctct tggcggtaga
     3421 ccccgctcgt gttcgcttcg atgtcgtatc tccgtttgga gttacgcttg ccacgctcac
     3481 ctccgatggc caacgcttct cgttttttga catgaaaaac agtgcgtttc tcgaaggacc
     3541 gccggaacct tgcaatatcg cacggctgac acaggtgcgg atgccagccc atgcgttagt
     3601 gcagttattg cgtggagaag cgccgatatt ggttcacaag ccggaggccg caacgattca
     3661 atggaacggt tcggggtatt atcgggttca gatcccgagt cggcatgagg cgatgcaaga
     3721 gctgcatctg gagccaaacc cttcggacta tgctcttccc taccagcagc agcgtgttcg
     3781 cgttttgtat gtacgcgtta cccagcggga ctacgttcac ttcgaagcct cgttattcga
     3841 tcacgccgac gcggtcacga tgcaagccag ggtcgacgag ttgggcatcg atccaccgat
     3901 tcctccaagc ggtcccgcat gtcgcgcgga gatacctcgt cgcattcagg tgcaagtacc
     3961 gtatacacac gatgatatgc gttttcgtta cgaggaggtg ggtctcaacc cgcctttacc
     4021 tgacgatatc ttcacacaac caattcccgg aggcgttcga cgacagtacg tgcagtgccg
     4081 ataagctctc attcgtccac ggttcgtgac aaggagttgc gattgatggc agagggttcg
     4141 accgaaccat cccgtttgtt ttgcatgcac tgtggaggca cgcatccgcc cggattgcgc
     4201 aggtgttctt ccacgggacg aagtcttggg ggggacccac gtctcatcgg ccagttcatt
     4261 gcgcgtcgct atcgtatcgt tcgagtcctc ggagatgggc ctttcggggc atcctacaag
     4321 gccgagcacg ttgcggtagg aaggttcgta tcccttcgca ttcttcatgc gggattggtc
     4381 acgagtcccg acgtgctcca tcgtttcttt cgcgaagccc gtctcgtggg ttccgtaacg
     4441 catccgcgtt tgcagcctgt cgtcgacgcg gggctaagcg acgacgggat agcgtacgtg
     4501 gcgtatcaat acgagcgagg ccgaagtctg gcgagggcct ttgcgcacaa tacggtgttt
     4561 tcgctcgagc aagcagcaac tttgacgagc gagattcttc atggactcga ggcgattcac
     4621 aactcggggt ttgtccatcg agcgttgatt ccagattccg ttttgctaca gcggggcgca
     4681 tcgggaacgg agcatgccat gctcaccaat tttggcgcgg cgactttcga aaaccgtgaa
     4741 gaaaacagtc ccgtgtcaac cgttggcctt cctccctttt cccgcggccc ctatctgctt
     4801 gctccccacg cctattgttt gaaaccggat cgtcgggagg atcttttcgc cgcgggtgtt
     4861 ttgctttcgg ccatgctttg tccaggaggc atcccgcgtt tcggaggtga tctcatcgct
     4921 ctcggcgtac ctgcggccct cgaagcgctc atagcacggg ctacccatcc tcgcattgac
     4981 gctcgttatt cgtccgcaag cgaaatgctg gcacagcttc gttccttttc ggagttggtc
     5041 cacgacgatg tggcatcggt gacagaaacg gtgatcaatg acctgcgcgc tcttcgcaat
     5101 cgggagcggg tgttggatgt cattccggcg cgtcttcgat tgggcagcgc aacgtcgatg
     5161 atggcatcgg cgttggccgc gccactcgtg cgagcattga aaaaagagtc cgcgcagcgt
     5221 tggaccgaaa tcgttcaccg cgtaccgggg attgacaaag tgctcgatct cacggcggac
     5281 atcgggccca ttgccgcaac gcccatcatg gcggccctgg aggaagccga tgccttatgc
     5341 ggcgtagatg accgattatt ctgtgtcatc gtgggggaac gtgcgggcaa agaagagttg
     5401 aacgagtggt ttcggacgat gttggacgct cctactcccg aatgtttatt tgatgggctt
     5461 tcgcgtctat ggcctagcat ggtgggccag ggcatcgctc gcgtgcgaca ggttgggcgg
     5521 ggatatggtc ggttcgagat tcgcgatcaa ctcgaaccat ctttcgccat ctgcacggca
     5581 ctcgcgggtg tgatcaaagc cggtctcgag cacctgggcg caagatccat tgaagtatcc
     5641 aagacggctt gtgaggcggt gggcgatcct gcctgcgtgt tcaatgtcac ctggctgagt
     5701 tgagcccatc cagtacacgg acggaatgcg ccttccggct ggtcgtgagg gcgcggacct
     5761 tacggcagat cagacattga aacggaagtt gaggatgtcg ccatcttcca ccacgtagtt
     5821 tttcccttcg acgcgaagtt tcccttgttc tttcacacgc tgttcgcttc ccaacgcgaa
     5881 aaactcatcg agtttcatga cctcggcgcg aatgaatcct cgttccatat ccgaatgaac
     5941 gcgccccgcc gcatggggcg ctcgatcgcc gcgtcgggta gtccatgctc gaacctcatc
     6001 gggtccgacg gtaaagaagc tgatgtagtc gagcagcgcg taggcatgac gaatgaagcg
     6061 agcggacgcg ggttccgaaa gacctaggct atccaaaaac tcaccttgtt catggaccgg
     6121 taacgcggcg atttcagcct cgatggctgc acaaacggac atcacttcca cgctccgggc
     6181 ggctcccgct tgcaccaacc cggacggcgg ctgctgcgtg gatttttctt ccggcacatt
     6241 caataccgcc agcatgggac gacgtgacac gaatccgtat gaggcgatca cgggttcctg
     6301 ctccacacgc aatggcatgg tacgcagggg acgcccttcg gaaagccagg caagacaagc
     6361 ctccagcgtt tccgtctcga tccccaaggc atgttcctta tggagacgtt cgagccgctt
     6421 ttccaccacg atctggtcgt tgatcaatag ttctgcttcg aaatcatcca gttcccgaac
     6481 agggttcggc tcgctgccat cgctgccttc aaacccacgc aacacgacag ccaacgcttc
     6541 tgcctcgcgc aaggattgca acgttggccc atccaatcct ccccctttcg ttcgcggtcc
     6601 tggaacatcc acgaagcgaa gctccgcata cacggttttt ttcggcttgt agatcgcgga
     6661 tagcttgtcg acgcgcgggt cgggtacctt gatcacgcca agattaggct tatcttgacg
     6721 acctgccccc gtcgtggctg cctgtcccgt cagcgcgttg aacagtgtcg tttttcccgt
     6781 ctgtgcatac ccgaccaatg ctgctttcat gttccctgac atcctcagat cgaacgagtc
     6841 ctctgtctcc tacttcacac gcccagggtt cgtcgaggtt tcgatccatg gcgcgatgcg
     6901 gttctgcttc acgtcgccga tcaccgctgc gcatctcgcc acacttcgat gtcacgcaga
     6961 gcccttgcgg ccaaagcggc ttttttctcg tgttttttga atcgcccttc gagcggcgga
     7021 acatgggccc atgtgatatt cgaaggctgg aatggtttac gcgattggct tagatatcga
     7081 agcaatccac ccatggtcgt ggtttcggga ggaagacaag gaattcgccc gttcagccga
     7141 tcgacaagca ggcgcgcgca cagcagtccg cacgcgcaag actccacata accttccact
     7201 cccgtgattt gccccgcaag gaaaactccg tccagcgcgc gaagttgtag cgtctcatcg
     7261 agtacggcag gagcatcgac aaatgtattt ctatggatgg ctccccatcg gagaaactcg
     7321 gcattttcga gacccgggat cattcgaaag actcgatttt gctccgggcg tgtcagccgc
     7381 gtctgaaaac cgacgatgtt atatgcggtt ttttcacgat tctcgagccg cagttgaacg
     7441 accgcgtacg gacgctcgtg ggtgcgcggg ttcatgagcc ccacgggttt catgggcccg
     7501 aaagccaagg tcatcggtcc ccgttgagcc atttcttcaa tcggcagaca gccttcgaaa
     7561 aactgtgatt gttcgaacgc gtgggggcga acccgatcgg cctgttgcaa tgccgaaacg
     7621 aacgtttcgt attgtgttct atcgagaggg caattcacat acgcttgcgc gtcttcctca
     7681 ctttgccgat cggatcggtc atagcgggat tgcttgaata cgatgtccca atcgatggaa
     7741 tcggcagcta cgatgggagc aatggcatcg tagtaatgaa gattgtccga acccaacaca
     7801 cgggacaaat ccgaggccaa tgcatcactg gtaagaggac ccgtcgcaag aacgcagggt
     7861 cgagccttgg gaatggcatc gaggtgttcc tgcacgactt cgatccgggg gtgcgaacag
     7921 atggcatgag tgacggcttg ggcaaaagac aagcggtcga ccgcaactgc gccccccgct
     7981 ggcacacgat gctgatgagc caaccgaacg atgagtgaat cacaagcgag aagctcttgt
     8041 ttgagggttc ccaccgcgtt ggcgacatcc ccgcttcgaa gcgaattgga gcaaaccaat
     8101 tccgcaaaca atccggtttg gtgggcggcg gtggttcgtg cgggacgcat ctcgaccaaa
     8161 cgcactgcga atccttgttc ggcgagttgc cacgcgcatt ccgagccggc caaccccgct
     8221 cccacgaccg tcacgatatt catgaaactt cgacagccat catcgttgtt gttgttgcct
     8281 tgctagatga taaaaaccgt cggcgctttc tagcagcaag ccgtcgaggg tcaaggtaag
     8341 gacggcagcg gagacttgtc ccgcactcaa acccgtgagt tcacatatcg catccaggtt
     8401 gttcgcgctc gaggacaacg tatcgacgac cgcctgttca tgatttgaca gcactcggca
     8461 gggcgagagc cgtttcatcg ctcgtccaag aagttgcgtg ggactagaaa gcacgcgcgc
     8521 tccggcggca atctcgagca aacagccttc cgcgacattt tcccacgggc ttgcaggaac
     8581 ggcccaaatc gtttttccca tgcgccgcgc cgccgccacc gtcgatcgcg cgccggatcg
     8641 tgcaggagcc tggacaagta cgacatggtc gacgagcgaa gcgagcaatt catttcttgc
     8701 caaaaatgac caccgtgttc ccacttgcga tgggggatag agggaaagcc aagcgccacc
     8761 agtttcgagc agttggtgaa acaaagcggt attgcacacg ggataaagat ggtcgaagcc
     8821 ggtgcccatc acgacgacgg atttcccatt ggcgcgcaac gctccttcgt gggcggcggt
     8881 gtcgatgccg gcggctcccc cggaccagac ggaccagcca gcttcgcaga gccgttgcgc
     8941 aagaagggtt gtgaatcgaa gggctggcgc ggtggcgcga cgtgttccta cgatggcgac
     9001 cgcccgggct tgagggaggg tgccgcgcac gaacaggcga gccggtgctt tggccatggc
     9061 gcgcacggcg atgggataat cggggtgtaa gggtgtaagg acgaagggat cgtcaggagc
     9121 catagccctg cttcggccat ggcggcgaaa agttgtggca gaagtgagta cgccgctatt
     9181 tcacgtacaa ttgagcacca acgggaccgg agccggtttt ggccgtgacg atgatcttga
     9241 cctgaatggg caagggcaac acgtgtttgt agcagtcggg agccggggcc atggtggcct
     9301 gggcacctgt tttcaggtct tgcgccatcg gcagattggg tgctccaggc agcggaatga
     9361 gtggtgccat gacgatatcc agttcttgga ccatgggcat tccgaggccc accacggtgt
     9421 agcacttacc gggttgaagc atcgcgatct gttcgaggga ttgtccctcc tgcagcaccc
     9481 cgctgatgat ttccccttcc tttttcatcc ctttggcatc ggtctgggcg atgggtacaa
     9541 gctgtgcggc tagcagttgt tgaactgctg ggtcgaaaat ggtcggcgaa ggctgggtgg
     9601 aggtcgccga ggcggtcgga gcggtctgag tcgttgtagg tgccgtggtt tggggctgtg
     9661 tatcggtcgg cgccgttgtg gtgggaacga acggttgagg tgattcaggt tctttcctgc
     9721 aggcgagcgg gacggtaacg atggcaagga cggccgctat ggccaacgcg ttgcgttgat
     9781 tcatgaatga ctccgttgtg atatccgagg ggtacgaagc atggtcctac taggattcgc
     9841 gagacaattt ctcgtaaggg caagtgatgt caagtcgcag aggcgacgac acctttgccg
     9901 tttttaggcg agtcatgcga aatcacttgg aataagcctg gattccaatc gttccggcac
     9961 cgcttttcat ggtgacttcg agtcgaacgg gcatggcaat gggcgagggg cttcgatagc
    10021 agttgccgcc accccaaacg accggcatgg ggttcatacc ggcggcgttt tggccgagaa
    10081 cctgctgggt gagcggggcg ggcatggtga ctttgatctc cgtttcgagc acaccaatgc
    10141 tggaggcacc gacgagggta taacacttgc ccggctgcag ggtgacctgt ccttccgagg
    10201 tttggccttg ttgcaaagac aatttgacca tggagccatc ggggttcata cccggcgcgt
    10261 tttgactagc attgtttcgg ataccgatgg cgatggggtc ggccgcgttg gtgccatcac
    10321 ctcccgtgcc gccaccgatt ccaccaagaa ccgcgccgac catgccggtc aactggccga
    10381 aaggatcgtt ggccgctggt tgcgtggtcg ttccggttgg ctgggtgggt tgcgttccag
    10441 gctggttggg atccgtgtag ccgggctgtc cgggttgcgt gtaccccggc tgaccataac
    10501 ccggctggcc gtagccgggt tggccgtatc ccggttgccc atagcccggc tggccgtagc
    10561 catattgccc ttcgaaattg ttgggcgtat tgggctcatc ctttttgcag gacgttacag
    10621 ccaaaggaag cgccgcaatc acggaaaccg cggccagaga aagaagcact cgcttgctca
    10681 tatccatatc ctcctgagaa gcctgatggc cgctcgatgc accaaacgta tcacggaaaa
    10741 cttatcttac acctgatatc cttatggacc gcaacagcac aatggcccga tggggtggaa
    10801 gcagacttca attggacggc atcttgccat aatttatgca cgctttcgaa tcggatcctc
    10861 acaacgggcg gtcattattc tgtccaccac catacagatg cataaggtca atgaacgtag
    10921 agccaagcgt gtggtagttg ccacacacgg gcattgtttc gatggcgcgg catccgctgc
    10981 ggttttcact cgatttttgc agtccttctg cgcggaaccc ctcgaattct cctacgttgc
    11041 ctgcggctat tccccccaat cgaggtgcga cccaaacgtg ctgctcacgg gagacatcaa
    11101 cgccatcctt gattatcgct acacacccca ccccaaccta cattggtact tcgaccatca
    11161 tcccacggct ttttcgaccg atgaagaccg cgcccatttc ctgcagcatc aaaaccatcg
    11221 caggtttcat gacacctcct acggaagttg cgccaaactc gttttcgaca tcgccgccca
    11281 acccttcgga atcgacttac ctgaactggc acctctcgtt tcctgggcag accgaatcga
    11341 tacggccacg ttcgaaagcg ccgaacaagc catcgcccgc gaacaccccg tgctccaact
    11401 catgacggtc ctcgagcatg cgggccacag cacaaccctc aaccaactca tccctcgtct
    11461 tgccgcaaac cccatcgaac aagtggcgtc cgaaccggcc gtgcgacgtt ccgcgaaacg
    11521 ttttctccga taccaccgcg aattcgtcaa agcagtccgg cgcaactgcc tatccttcga
    11581 tacggtcgtg ctggccgatc tctccagcga attgcatgaa gtcgctggaa aattcgtcac
    11641 ctatgccttg tttccacgca gcgtatactc cgtgatgctc acgcggggtc gtcatcatac
    11701 gaaaatatcg gttggatata atccctggtc tccgtttcct cgacgccatc acatcggaat
    11761 gatttgcacc cgatacggcg gcggaggaca cgctacggtt ggcgcaatag ctcatcccgc
    11821 atcttgcgtt gaccaagcga aaacggtggc acgacaaatc gtcgcagaac tcgcgtcttg
    11881 aaagatcata atggggtcga cgggggtcgg agctgaccaa gcccggctgg agttggcacc
    11941 ttggcttcca cgtttcgaac acgcttggcg agcgcttcga gaacccgcaa gctttcttcc
    12001 tcccccgcgg catgggtgac atcgaggccc tcgtaataaa gaccatattt ttcggttcgg
    12061 acgggaggcc gtggggtgtc ggagatcaag gcaatcacca gtcgcattcg ctgacctcca
    12121 gcctccaaac gaatttccat tcctccatcg gcgcgcacgg ccccgatcca caccacctca
    12181 tagccttcga gttcatcgcc caccccaaga ccatcgagaa gagcgagttg ctgcgcactt
    12241 gcaggcgccg atgacgaacg aaccatggga atcgtctcgt ttcgcacggt cggctcgtta
    12301 gccccgtcgg cttgattcga ttggggtcgg cacgcgaaga gcatgaccgc gaccgcaaag
    12361 ctcgatagcc acgaatagct cctcatccta tggcatcctg tccttgtgct acgccaagcc
    12421 gaagtcaaaa attagcagaa acgatgttct ccaaccagga atcagccgag ccatcggcgc
    12481 aacaaaccct tgaaaatgcg aagaaacgct tgggtcaaat cgtcgacaac aaatacaaac
    12541 tcatctccgt cataggaatt ggagggatgg gtatcgttta cgaatcggag catcaattcc
    12601 tgggacgaca cgttgccatc aagattttgc atccgcgata tgcggatcgg ttggaggaat
    12661 accagcgatt ttgtcgggaa gcacgagccg tgggggctct atctcatcga acgatcgtgc
    12721 aggttttcga tgctggattt ctcgatggca ccacgccgta tttcgtgatg gagcgactga
    12781 ttggagaaaa cctcgagcaa acccttcgtc gcaacaagat gctgaccgca cggcgagcct
    12841 tttggatcgc caatgaagtt cttcgcggcc tagcggtagc acattcgaaa aacatcctgc
    12901 actgtgacat gaagccggca aacatctatg tggttcgcca aaacgatcgt agagaaagca
    12961 tcaaattgct cgactttgga gtggccaaac tcgccgtgga aggctccgcg gcatcggtgg
    13021 acgagcgcac gggactcgtt ttcggtactc cacaatacat ggctcccgag caagtcacgg
    13081 gagccgaact cgatccgaga acggatatct atgcggtagg aaccattctt tacgaaagct
    13141 tgatgggcga gcccgcgttc cagggttcca caagggaaaa agtatttcga aacgttcttc
    13201 agggaaaacc caaagccctc attccaccga ctggccgtct tgcacccgcg ttacaacaac
    13261 tcgtctcgag catgatggcg aaaaatccag caaatcggcc gtcaagcgcc aaggaggtgt
    13321 cccggttcat ccttgcacaa ggagtgatcg attcaccaaa cgatcacgac taccgatgaa
    13381 tcatcctggc gattcagcca gaagcgcgaa aatggagcgg gcgctttcaa gaaggcggag
    13441 gtcccctgcg cggaaggaaa agacacttca atccggcctt atcgtccgaa caaggcgatc
    13501 gtgacaaatt gcttcgaacg ggttgacgaa cggcacgcac tcatcgaaca ttcccgccat
    13561 gcacgagcag aacaaagcaa ttctcatgtc attggttccg gtagcgtggg cggatggcaa
    13621 cttcgcggac caagagaaag aagtcctgga agccttgctc gaagcgttcg aagccaaccc
    13681 cgaggaagcg gaagaactgc gaacgtacgc ggccactccg aaaacggtcg acgatatccc
    13741 cgtctccgaa ttgagtttcg acgatcgtcg cgtgttgctg caacacgctg ttttgctcag
    13801 cttcgccgac ggagatccat cggagacgga gcataaagta attgatgcga tggtcgagaa
    13861 gctacgcatt cctcaagatg aggcatcggc tcttcgacaa accgccacgg agcgtgccaa
    13921 gcgatatctg aatatgcgct aaaccgcgat tgtcgacagt gtaggcgtat tagttttttc
    13981 cgatttgcac cttgagggtc tcgatttctt tgagcagggc aatcggacta aatggcttgg
    14041 tgtaataggc ggtcgctccc gcattggccg ccttctcacg ggatgcaagg tcatccaacg
    14101 cggaaatgat cacaatcgga acatcgcgtg tcgatggatc catgcgaagc cgacgacaca
    14161 cctcgaagcc gtcataatgg ccgggaaggt tgagatcgag caatacgata tcggggcggt
    14221 catgcatgca tacttccaag gctttggcac cattggcaac gccaacgacg tcatgccctc
    14281 ggcccgtgac cagagcctcg atcattcgtc ggatggagtc ttgatcctcc acgacaagga
    14341 tcttcatatc atctgcttgt ataggaaaac gaacccgcaa gcgagctttt cggtaacaga
    14401 cggcgtcacg gggcgggaat tcgcgcaagg acacgcgtcc cgccttctgg agaatccgct
    14461 atttctaaat ttcctcgaac agaagataaa attgaatata ggctcaacag gtgcacgcca
    14521 cgcggtcttc ctacggtaga cgggtcgatt cgaagccaaa tcagcgcatc acgaatgcct
    14581 gaaggcaccg aggcccctcc atcatccaca acgaaaacca ggtgccgctg ctcgatacag
    14641 gcttcgagat ggacgactcc gccgcgcggg gtagcggcga taccgtggtc gaggagcaaa
    14701 cggatgatgg tttcggtggc cgaaggcgga acgacggcat cgagtttgtc ggcgagggag
    14761 gattggagtt ggacaccatg gcggtcggcg cgaacggaag catgctccac aaggttgtgg
    14821 acgatgggcc gcacgtccac aagcgcggga aactcgtcgg gcttgacttg catgacccgc
    14881 gttacgtcgg aaagaagttc atgcagttgt gcgtaacagg tttgggtcgc ttccaaaaag
    14941 cgagactccg ctgatcgtcc tttgaccgat gcgttttcga tttctttcga gaggctggcg
    15001 agtgtggttt tgagttggct tgccacacca gaagcgaaag cgatgatggg ggacaacgca
    15061 tcttgaggag gaggcaccgt ggaaggtgcc accgaagcgt attccgcgga atctccggcg
    15121 gcaaaaaccg cggcgagttc gcgagcatag gcttctccct gctgttgagc ggcgtgcaat
    15181 tctttgcgaa gcgcttccat ttccttgcgg tcatgaaggt cttcggcggg caaaatgaga
    15241 gattcctgcc ccccctccag ttccaaacgt ccgccatagc ggattgccat gcgtccaagc
    15301 catgctcgtt ctgttcggtc cccagagaga gtttcgggac cgagagcgac agacacgcgc
    15361 acttcatcac cttcacggct gatggtgact tcaggagctt gtacttcccc ggcgagcaag
    15421 ctggtttgtc cgacgaggat ttgaaccatg cgtcgtagat ctccctcatc gccaaacact
    15481 tccgttccac ttcccggctc gaggcgcacc ctggcggaag ggatgatttc tacgatgaga
    15541 gcggccaggt cgactcttcc tcgagaaccc cgcttgctca tactcgcatt gagggagtcg
    15601 agcatgcgca tggcatcatc caaagcatcc agcgaagatt cgatttcggt gggcgcctct
    15661 tcgatggtga tttccggcgg gctatgcgac agccgggcca cgccttttcg aagcgttttt
    15721 gccgctgctc gagcctcttg ggtgagcagc cagctcagtt cttgtttcgt caagcggtca
    15781 ccctgagaca cggctcctcc tcgttctatc ccgttgccgg gcatcaaaga ctccgctgct
    15841 tatcatgcca gacctgctcg tcagggggaa cgggcttaca gtctttctct agcaaacgcc
    15901 ccggccctct cgaaccccat atcccaccgc tccatttttt tcgcccgttc gaacacgcaa
    15961 acccaagccg tttcgccacg tcgacgacgc tcgctgctca tgctaacgac cctttcatgc
    16021 atcgtcgctg tagcgaacgg acccataacg attcttcctt accgactagc tcgatacgct
    16081 caacccgtcg aagttgcccg agcggtttga cacatccgct gtggtggagc gcacttttgc
    16141 tgctggtgct aaacgaccat gttttcaagg gctcgggagt cattccgagc gtgctgacag
    16201 gaaaactcag cgattttgcc ggaatgatcg tggctcccgt cgtgttatgc gccctgtttc
    16261 gaattcgaag ccgccccgga accatcgtcg cctacgccgc ggtaggcatc ctattttctg
    16321 ccttgaacac gatccctgcc ctggccgacg ccttcactca ttggctggcg ctcatcggca
    16381 tccccttccg gatctggtcc gatccctccg atctcgtcgc tcttcccgcg ctggccattg
    16441 cctatgcact gttcggatcg cgtccttcaa cggcaccagc ctggtggatt cccaaagtca
    16501 acaccgcggt ggcggcgatc gcttgtgtcg catcgtctcc ctggcccagc gtatctcctg
    16561 ccacggtggc ccaaaatcaa gtgctcatgg agcatcatgc cggagtcgtt ttcgttctcg
    16621 atgccccctc tggcaccatg gtgcgaagtt ttcgcgcatc catcaccgat cgattgcctc
    16681 ctgccattca cgaccgaatt ctctatcaag ttcaacccca aggacgcatc accggcatcc
    16741 atatcgacaa tccgcaggag cccttcgagg tgtggaaaca tccagaacaa agcgctcgga
    16801 ttcgcatcag ggccgtcgac gcgtttcgcc tgtacgtggc gggaccagaa gatcggctct
    16861 atgccatcga tcgtgtgttt ggctcgttgc gttggaccct gcccatcgac acgtgccgtt
    16921 tgcaggactt gaccatcacc gatgatacca tgatcgtaac cgacggaccg cgttgtcggt
    16981 ccgtggatgc tcgaacggga agagtccttt gggaatacct cgcgccaaac acggtgggag
    17041 ccgcaacgat ccaccgcgag cacgtctata tcgcgagttc agacggtatc ttgtgggggc
    17101 tcgacgtgag cagcggtcgg cagcgatggc gctatgatgg tggcgcggat gcttgcgatt
    17161 cctaccatcc atcggttttc gcccaggaca actccgtgtt tgcctgtttc gatggacaaa
    17221 cgcgcgcgat atcagtgcag gaccgttcgg taacgtggaa acagcagggt caacccgtgg
    17281 ctatcgcgcc atccttgttg ctccttcaat cttccaacga caccttgcat ggtgtgctaa
    17341 cgacgactgg caagatcgcc tggacaactc ggttcgacga atccgtttcg gctcgacctg
    17401 tgatttggca gtcgcttgca ttcgttcgtg atgatgaggg acggctattt gccctggact
    17461 tgcgttcggg aacgaagcga tggaattttc tttggccgga tgacgctacc tcatcggcct
    17521 cgggcacgtc gctggtggtg acgactccat gatcggtatt tcccattctg attgatgcat
    17581 gaactcgctt tacgcccgcc gccttccacg accg
//
LOCUS       DAOSOR010000040        17131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_3360_length_17131_cov_4.250468, whole genome shotgun sequence.
ACCESSION   DAOSOR010000040 DAOSOR010000000
VERSION     DAOSOR010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 17131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17131
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3360_length_17131_cov_4.250468"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PK710_03745"
     CDS             complement(<1..390)
                     /locus_tag="PK710_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08856.1"
                     /translation="MKHRFMLGTTLAALALGILSACGKGSLVSPLVAEESSVSDTAQA
                     HLLLVPSADPESLLGRAVHMTGDNVWTIAQSRAAGCDVTVKRMPSRFDTRRNVQLSSL
                     TALAGGLPQLIEIEGRYGRRVTVHTEIQ"
     gene            512..1093
                     /locus_tag="PK710_03750"
     CDS             512..1093
                     /locus_tag="PK710_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08857.1"
                     /translation="MRIYQLGKLIVLPLFVACSGSTVKDADEPDAQPDVQTDVGKTDG
                     QQEAQADVASEPEPCVPEGSWNLTLIRSDGVSTQEQIQIAADGGQDETTHEIVFVDRV
                     VPTNSCTYPDASISDAAPETQTARGTLEESSCTLRLGFTNRWCSSGEDQCEQFNISLS
                     FQGDRATGGADSEGGWCLDKGHWTWEAKATRIP"
     gene            complement(1400..4510)
                     /locus_tag="PK710_03755"
     CDS             complement(1400..4510)
                     /locus_tag="PK710_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT08858.1"
                     /translation="MKIFYKISAILLVAFLFPLLLLGMTGYLSVREIGDRTTETTTEV
                     LLRTERMHLQKRASAEALELDNLCSSFENGVKHLRSHYQVLHRHRERYDVDKLPRAYT
                     GVDTAGLAGFGYVHPVYGAYADFDGKIPGAPWVPRPVVRKLRHDPALRTSIEASLESV
                     MLFDMHLQLAIELQEISADLAWIVLTNGVTNVRPEYDYNRIIAKNPSILDLDESTEDY
                     VRLVDAEHNPERKLLWIEPYFDQFKNTWMTSCVAPLYDGDRFLGSVGMDVLLSVIANQ
                     FIAGQEADGYAFLVSKAGKVLAISKDGIEDIAWDSAHRKALMQTFLNGRELPWTEEMV
                     ESYGSTTLDKSPEHDLREIVGAMKNGQTDIRNIRLSGQQKLIAFAPVRATGWSLAIVV
                     PEQAVASRANEVQTAFSQGIQTTVVRYSMTSLVVLFVSIAVGLILHYYAVRPLNQLSR
                     RVEAVRWDNLRLSKDPKPRDDEIGHLYAKFHEMLDVLRSARNETVGKAEEIQRINAKL
                     EIANQDLARELDERVLVEQSLSREKELLAVTLRSIGDGVITADRLGRVVLMNRQAEQL
                     TGWTQAEAEGLALDEVFVVRDENDQPCNVLARTLRTDEQEIGSFEVFLQARDGSKRLI
                     SDSGAPIRDRDGVEVGVVIVFRDVSVRKRLEEEMIRSQKLESVKLLAAGIAHDFNNLL
                     TAILGNISLSKRDPALSDRTRERLQDAEKASDRARGLTQQLLTFSSGGAPIRGPVDMA
                     RLVEESAAFALRGSNVALETSFAPDLQPVFADRGQIAQVIQNLVINAYQAMPTGGTVR
                     ITVDNTIVKPEDALPLQPGAYVRVSVKDDGIGISPENLRGVFEPFFSTKSYGNGLGLA
                     VCFSILKRHGGHIVIDSELGVGTECRFYVPVATEALKPKQGETATPPRGTGRILVMDD
                     EPMIRQLAENMLTSLGYEVETACDGIDALQKIEQLAAKGKPFDAVVLDLTVQGGVGGQ
                     EIISKLSERYPTLPAIVSSGYSNDPVMADFASYGFRGAIPKPYNLRAMAETLAMVLSA
                     NDDKVS"
     gene            4674..5573
                     /locus_tag="PK710_03760"
     CDS             4674..5573
                     /locus_tag="PK710_03760"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOT08859.1"
                     /translation="MPRDDLFAQPLLLDGATGTELERRGLQTKLPLWTALAARDVPEL
                     LLSVHQDYVHAGADILTACTFRTSRFTLSKSGLSDLAWPITQKAVHIARQATVGTSKK
                     LLVAGSIGPLEDCYAPAKAPHDAILEREHETHANHLARAGVDLMLVESMPTAREALIA
                     TQAALRTGLPVITSLLARRHGLLFDHSPLRSVIAQLALLPIQLVSVNCCSLQACDEAI
                     PYLSACGLPWGLYPNASDPDGSFGGTIKDLDRDLFRSAAQSWFHRGASMIGGCCGTRP
                     MHLAVLDSLRHTLLTPQPSKPRA"
     gene            complement(5700..5981)
                     /gene="secG"
                     /locus_tag="PK710_03765"
     CDS             complement(5700..5981)
                     /gene="secG"
                     /locus_tag="PK710_03765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOT08860.1"
                     /translation="MFETILSIIHVFACFFLILVVLLQQGKGGGLGAAFGASTTRQVF
                     GGRGAGNLLTRLTAVTAAVFMTTSVVLAYIASSGDRSLRATEAEVEKMP"
     gene            6643..7671
                     /locus_tag="PK710_03770"
     CDS             6643..7671
                     /locus_tag="PK710_03770"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOT08861.1"
                     /translation="MTRIPPHERPEIERPDDVDDKAVLSVFRVPKELAGMRADVFLST
                     QLRNTSRTRAKIIVRAAARFLDGSRIKPNTRLAPEQYIALWRPAWDETEIPFDIPILF
                     QDEHLLVIDKPAGLPVHPSARYYRNTVIKKLGERFPSEHLVLGHRLDRDTSGVLVLAR
                     TIEADRLLKKQFRNVLGVEKSYQAITWNTPPYPQQRIELPLERNPESRLRCTMRVAAP
                     GTGLKAATRIGIIKRYRRGDRRYSLVRCDLETGRQHQIRVHLASLGCPVVGDKIYGPS
                     EELHARSSDGTLTDDDRLQLELPRHALHAWRMAFMHPMTDDRLVFEAPLPEDMQAFVR
                     GLEVDDTL"
     gene            complement(7705..8508)
                     /locus_tag="PK710_03775"
     CDS             complement(7705..8508)
                     /locus_tag="PK710_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HOT08862.1"
                     /translation="MDWSALLDRIGISSLPNDPWQRLVVLHQAIPRHVPFENIAILEG
                     KPIVLTTEAIFEKIVMQRRGGYCFEINGLLLLLLESLGYQVDRLLGRVWSGSATTPAL
                     THMALRVTVDGQQYLCDVGFGAATLRAPLRWQFFSPEQQQLDTYRLEPTDNHEVQLSV
                     LKEHGWKPLYSLLPCCVRAQDYIPANHYTSTHPMSYFTQEPVAALATEQGRVTLRRDR
                     FAIQRGSQRIEKQINEINELKQILEEHFGLMNLDWHTLRTRLQGIFPAR"
     gene            complement(8662..8979)
                     /locus_tag="PK710_03780"
     CDS             complement(8662..8979)
                     /locus_tag="PK710_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08863.1"
                     /translation="MSQEQGDYRATARSVLDRDEVQDVLSGAFYTPPPEQRGHRAKPV
                     SKPEKPAHYKVICISMYTEDLGKLDEMVDELKARGLTKANRSALIRYALSQVDLNAVP
                     KGL"
     gene            complement(8994..10364)
                     /locus_tag="PK710_03785"
     CDS             complement(8994..10364)
                     /locus_tag="PK710_03785"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOT08864.1"
                     /translation="MARMTESCSVCGNSFEVQFRYQMEESEDGFSFYCSQKCLETSQT
                     ANGQGLMTCDACRKHFRVQLVSQVLYVADRRKYACSLPCREQILDEARGVRLGDMANG
                     VLQPPGPTEPLSNETAEPKIMPTMPVASNASVASDPPTAYSQAAHQNLPENRARLSAA
                     EPVSLIPTIEARTAPPVQNAPSKRSAMVFAIFNHKGGTGKTTTSVSIAAGLAQTGRRV
                     LLVDTDAQGNVGVSLNKNADKSLYHALVMGLPLKTVIQSVRENLDLIASNETLAAAEL
                     YLAGRQNRDRVLRDRLSPAVRGYDFIVLDCSPSLSLMNQNALVFADNVLVPVACDYLS
                     LVGVRQVIKTVRNVNQLLHHPVKLWGVLPTFYDARANICREAVETLQKHFGDRCLPPI
                     RSAIKVKEAPAHGQTIFEYAGASTAADDYRTIVNQLIMDRDSSLGVTTGATMEPATMA
                     AAAVTA"
     gene            complement(10546..12099)
                     /gene="purH"
                     /locus_tag="PK710_03790"
     CDS             complement(10546..12099)
                     /gene="purH"
                     /locus_tag="PK710_03790"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOT08865.1"
                     /translation="MNTRRALISVSNKSRIVPFAKSLSNLGFELLSTGGTAKLLVDEG
                     IEAIPVERYTGSPEVMDGRLKTLHPAVHGGILARLGRDENDLAKVGATPIDMVVVNLY
                     PFEQTVSRADASDAEIIENIDIGGPAMLRSAAKNHQRVVAVCDPDDYDWVAQELQHGD
                     LDLGKRRQLAAKVFAHTAAYDAAVARYLSTDADAQGPEPFPRFLTLPFERGYHLRYGE
                     NPHQKGAFFRERHAPIGTIAMAKSLGAGGKELSYNNFIDLDAALEAVREFQSPAAVVV
                     KHTNPCGVATSDSLEKAYRLAREADSVSAFGGIVALNHNVDAATASALAETFLEAVVA
                     PGFSEEALAILRKKKNLRLLDCGVMLPPDHPSDVLRRISGGIVVQQRDTTAQGEVEAA
                     AVVTNRAPTQEEMLALSFAWKVVKHVKSNAIVLAKPNVTVGVGAGQMSRVESVMIACR
                     KAGEASRGAVLASDAFFPFPDGIELAAAQGVGAVVQPGGSVKDQDVIDAANRAGIAMI
                     FTGKRHFRH"
     gene            12285..>12757
                     /locus_tag="PK710_03795"
     CDS             12285..>12757
                     /locus_tag="PK710_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08866.1"
                     /translation="MRNTSWIVVGLAAIALAVGCTTKSEEDTGPASGGSSGGGYTPTP
                     NGVGIGEQEACEMLRDAFDKHASRLSCTYTLPECPAYIRAGQAPVCSKYDEGTVKGCA
                     DFYAGFTSCSEFQKRPCHIMNIEGSAPTGCPDDAGVSDSEEPTEAGEPDAEEQVDG"
     assembly_gap    12758..12857
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12990..13766)
                     /locus_tag="PK710_03800"
     CDS             complement(12990..13766)
                     /locus_tag="PK710_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOT08867.1"
                     /translation="METVSARADQVSVRVQDRTCETRNKYMVDLEIPRMDNNNHFDNM
                     ESLPQASSPANDGTQSALAAPMTREQRRSRRKRDVRARTISVRRMTKKELEIGRLLYP
                     ETDYWKPRTRAECVDGLRPCPFVSCRYHLFIDVSDKTGAIKLNFPDLEVEEMIESCAL
                     DVADRGGATLEQVGAIMNLTRERIRQLEVNALAKLQLLKEMETLRDYVDEGPHGKRRL
                     PMLTADDFEEPDRVEETDDDEDSEDALDHADSISFDDFGA"
     gene            complement(13908..14435)
                     /locus_tag="PK710_03805"
     CDS             complement(13908..14435)
                     /locus_tag="PK710_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOT08868.1"
                     /translation="MGVGGIDQSVLRRIEDEYALGISSADILDLLASLGIKFSEATLR
                     KWVQLGLLPRSVRVGIKGKNTGSHGMYPVTVVRQIIEIKELLSDGLTIEQVQQNVLFM
                     RGELEELRRAIAKVFVTLEAVVDKVESQSLRNSVSVDLNHARAMADQLLERLANVETN
                     LARRCEIMEVPAATG"
     gene            14984..15820
                     /locus_tag="PK710_03810"
     CDS             14984..15820
                     /locus_tag="PK710_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT08869.1"
                     /translation="MLISPIARDVTARRIRTRILQSGSGSPLVLIHGFLSNHHTFDDV
                     VPSLARSFQTVTIDLPGTGESEKPPPSRFDYSIEAFAEVVTDVIAALQIGRCHVVGHG
                     LGAAIALALASEHPEFVDHLILVSPEIYSGPPPPLTRIMRMPLAGRFLFKQVLGRSLF
                     HSLYLKHQYAPGHIVQSERIDSYYGPFIEPAARESAFAVLRAQLDMRPSIARLSRIKS
                     RTLVLWGRADRRLIAEYAPRLVRQLQLAQLEYIETGHTPHEEKPDLFVASLERFILGE
                     RS"
     gene            15817..16683
                     /locus_tag="PK710_03815"
     CDS             15817..16683
                     /locus_tag="PK710_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT08870.1"
                     /translation="MTTSPLLPRLFRSRSLILGSGLVVLLVLVAIVGPWFVAHDPNHS
                     DFATGQGTFGLPGGPNRHHWLGTDTLYRDLLARLLYGARISLFIALLATTLAVTVGTL
                     VGLSAGFFHGSRLRFVDTWLMRTVDAGLAFPYLLLVMAVGAAVDRSDTTTIIMVLGLT
                     GWLGTSRLVRAKTISVRQLDYVVAARALGQNPIIIAIRHILPNIAGPILVTASASVAS
                     MILAESVLSYLSVGIQPPTATWGRMLREGQTFFTAAPRLVAAPGVAILLAVLAFNLMG
                     EGFRDTLDPRTR"
     gene            16655..>17131
                     /locus_tag="PK710_03820"
     CDS             16655..>17131
                     /locus_tag="PK710_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT08871.1"
                     /translation="MTRSIRALANPLVLWLWLLGCQDRLNQPQGNPTEGPAQNGGTLT
                     MSTFVHIRTVDPAVAFDEASEPIVRLLFSRLFRVSRTGQIEGDLVAHHELSPNGLELT
                     MELRRDARFHDGSPVLAGDVKRSFERAFHPKTPCPVPSFFDRIVGLTEYRKGKSSEL"
BASE COUNT         3676 a         4940 c         4503 g         3912 t
ORIGIN      
        1 ttgtatctcc gtatgcaccg tgactcttcg gccgtaccgt ccttcgattt cgatgagttg
       61 gggcaatcca ccagccaagg ccgtgagaga agacaactga acgttgcgac gagtatcgaa
      121 tctcgaaggc atgcgtttga ccgtcacatc gcagccggcc gcgcgagatt gcgcaatcgt
      181 ccatacgttg tcccccgtca tatgaaccgc tcgaccgagt agtgattccg gatcggccga
      241 aggcaccaaa agcaaatgcg cctgcgcggt atccgacacc gaggattcct ccgcaacgag
      301 cggcgagacc agcgagcctt tcccgcaagc cgataggatg ccaagggcca gtgccgccaa
      361 agttgtcccg agcatgaatc gatgcttcat tacctatcct cgttcgtccc ggccaccttc
      421 agcggcatca tcgcgtcaac atacgaccat ttcttacttt tcttccttgc cacccctgcg
      481 tgggcaacct ttggggtgct acaacacgtt catgcgcatt taccaacttg gaaagctgat
      541 tgttttgccc cttttcgtgg catgcagcgg ttcgacggtc aaggacgccg acgaacccga
      601 cgcacaaccc gatgtgcaaa ccgacgtcgg aaagacggat ggacagcaag aagcgcaagc
      661 cgacgtagcc tctgagccgg agccttgcgt tcccgagggt tcgtggaact tgaccctcat
      721 tcgcagcgac ggcgtgagca cgcaggagca gatacagatt gcggcggatg gtgggcaaga
      781 cgagacgacc cacgaaattg tgtttgtgga tcgcgtggtg cccacgaatt cgtgtaccta
      841 tccggatgca agtatttccg atgcggcccc cgagacgcaa acagcgcgcg gcactctgga
      901 ggaatcgagt tgcacactgc gtttgggttt cacgaatcgt tggtgttctt ccggggaaga
      961 tcaatgcgag cagttcaaca tatcgctctc gttccaggga gatcgcgcga cgggaggcgc
     1021 tgatagtgaa ggtggatggt gcctcgacaa gggccactgg acttgggagg ccaaagccac
     1081 ccgtatcccg taagcttgcg ctttggcggc tccttcgcag acgagcccac ggggaagaag
     1141 tcatcccccc ttcagtagta acatattgaa tttacagcat attaattcgc ccgcaatcag
     1201 ggcattccca cgtaaccttg ttgcaagaca tcccccattc gttttccatc atgcaaggga
     1261 gacaaatctg gaaggggcaa tcgacgcaag caaaacagaa gggcaaggat ttctcgcata
     1321 gggtgcagtg gaagagcttg cgttttcgtc gctgctgacc cttcgtatgg ctcacgacat
     1381 cactccgtta tgcgttcagt cacgatacct tgtcatcgtt cgcggaaagg accatcgcga
     1441 gtgtctcagc catggctcga aggttatagg gtttgggaat cgcgccacga aacccgtagg
     1501 aagcgaagtc tgccatgact ggatcgttgg aataaccact cgatacgatg gcaggcagcg
     1561 tcggataccg ttccgacagc ttgctgatga tttcctgtcc ccccaccccg ccttgcaccg
     1621 tgaggtccaa aacgacggca tcaaagggtt ttcccttcgc ggctagttgc tcgatttttt
     1681 gaagagcatc gattccatcg cacgccgttt ccacctcgta tcccaatgat gtcagcatgt
     1741 tttcggccag ttggcgaatc atcggctcat cgtccatgac cagaatcctg cccgttcccc
     1801 gcggcggcgt tgccgtttca ccctgtttag gtttcagagc ttcggtagcc acgggcacat
     1861 agaaccggca ctccgttccc acgccgagct cggagtcgat gacgatatga cctccatgtc
     1921 ttttcaagat cgaaaagcag acggcaagac cgaggccatt gccataagat ttcgtggaga
     1981 agaaaggctc aaacacccca cggagattct cgggcgagat cccaatccca tcatctttga
     2041 cagagacgcg aacgtaagct cccggctgca ggggaagggc atcctctggt ttgacgatag
     2101 tattgtccac ggtaattcga acggttccgc ccgtgggcat ggcttggtac gcgttgatca
     2161 cgaggttttg aatgacttga gcgatttgac ctcggtcggc gaatacgggc tgaaggtccg
     2221 gtgcgaaaga ggtttccaga gcgacgttcg aaccacggag cgcgaaggcg gcggattctt
     2281 ccacgagtcg agccatatcc accggaccac gaatgggagc ccctccactc gaaaacgtca
     2341 gaagttgctg ggtcaaaccg cgcgcgcgat ccgacgcttt ctcagcgtcc tgtaaccgct
     2401 cccgtgttcg gtccgacaat gcgggatccc gtttcgacaa cgatatattg cccagaatcg
     2461 cggtgagcaa gttgttgaag tcatgggcta tccctgcggc aagaagtttc accgactcga
     2521 gcttttgcga tcgaatcatc tcttcttcta atctttttcg gacgctcacg tcgcgaaaca
     2581 caatgaccac gcccacttcc acgccgtctc gatctcggat cggggctcca ctatcggaaa
     2641 tcagccgttt gctaccatcg cgcgcttgca aaaagacctc gaacgacccg atttcctgct
     2701 catccgttcg gagtgttcgc gccaagacat tgcagggttg atcgttttcg tctcgaacga
     2761 cgaacacctc atcgagagcc agtccttccg cttccgcctg ggtccacccc gtgagctgct
     2821 cggcctgtcg attcatgaga acgacccgac cgagtctatc agccgtaatc acaccatctc
     2881 cgatgcttcg aagggtgaca gcgagaagtt ctttttctct ggacaaggac tgttcgacga
     2941 gcacgcgttc gtccagctcc cgtgccaggt cttggttagc gatttccaac ttagcgttaa
     3001 ttcgttggat ttcttccgcc ttgccgaccg tttcatttct tgcgctccgc agcacgtcga
     3061 gcatttcatg aaacttggca tacagatggc ctatctcgtc atcacgcggt tttgggtctt
     3121 tcgacaacct caggttatcc cagcgcaccg cctccacgcg ccgcgacaat tggttcaacg
     3181 gcctcaccgc gtaataatga agaatcagcc ctaccgcgat gctcacgaaa agcaccacca
     3241 gcgacgtcat cgaataacgc acgactgtcg tttgaatccc ttgggaaaaa gcggtttgga
     3301 cttcattggc ccggcttgcc accgcttgct cgggaaccac gatggcaagg ctccatcccg
     3361 tggcacgaac cggcgcaaaa gcgatgagtt tttgttgtcc agaaagacga atattccgga
     3421 tatcggtttg tccgtttttc atcgcgccta cgatttctcg aaggtcatgt tcgggacttt
     3481 tgtcgagcgt cgtgctcccg taagattcca ccatctcttc ggtccatggc agctcccgcc
     3541 cattgaggaa ggtttgcatc aaggcttttc gatgcgcgga atcccaggcg atatcctcaa
     3601 taccatcttt ggaaatcgcg agaacctttc cggctttgga taccagaaac gcatatccgt
     3661 cggcctcttg accagcaatg aattgattgg cgatcacgga tagcaagacg tccatgccga
     3721 cgcttccgag aaagcgatcg ccatcgtaaa gcggcgccac gcaactggtc atccatgtgt
     3781 ttttgaactg atcgaagtac ggttcgatcc acagcagttt ccgttccgga ttgtgctcgg
     3841 cgtcgacgag ccgcacgtag tcctcggtag actcatcaag gtccaaaatg ctgggattct
     3901 tcgcaatgat tcgattgtaa tcgtactctg gccgaacgtt cgtaactcca ttggtgagca
     3961 cgatccacgc cagatccgcc gagatttcct gcagttcaat cgccagttgg agatgcatat
     4021 cgaacagcat caccgattcc aacgacgctt cgatcgacgt gcgcaatgct ggatcgtgtc
     4081 tcaatttcct caccaccgga cgcggcaccc aaggtgctcc cggtattttc ccatcgaaat
     4141 ccgcataggc accatacaca gggtgtacgt agccaaaacc cgctagcccg gccgtatcga
     4201 ccccggtata ggcacgcgga agcttgtcga cgtcataacg ctcccggtgc ctatgcagca
     4261 cttgataatg agagcgaaga tgcttcactc cattctcgaa agagctgcaa agattatcta
     4321 actccagcgc ttccgcggaa gcgcgtttct gcaaatgcat tctctctgtc cgaagcaata
     4381 cctccgtcgt ggtttcggtc gttcgatccc cgatttcacg caccgacaaa tagccggtca
     4441 tccccaacag aagaagaggg aagagaaatg cgacaagaag aatcgcggaa atcttataaa
     4501 agattttcat gttcgatagc ccggaggggc gacaccttcc atgccctaaa acagtatact
     4561 tcaccatcga attgaggaag ggtgagacag caagacgagc ccattgttcg gacgcttcgc
     4621 gatttcatct tggacaaact cgctcacctt caccctccac ccgagacttc cccatgcctc
     4681 gagacgacct attcgcacag ccgttgctcc tcgatggcgc cactggtacc gaactcgaac
     4741 gtcgcggttt gcaaaccaag cttccgttgt ggaccgcttt ggccgctcgc gacgttccgg
     4801 aattgctttt atccgttcac caagactacg tacacgccgg ggccgatatc cttaccgcct
     4861 gtacctttcg aaccagtcga ttcacactat ccaaatccgg attatccgat ctcgcctggc
     4921 ccatcacgca aaaggccgta catatcgcgc gacaggcaac cgtcggcaca tcgaaaaaat
     4981 tgctcgttgc cggatccatc ggtcctttgg aggattgtta tgctcctgcc aaggctcccc
     5041 atgacgcaat tctcgaacgt gaacatgaaa cgcacgccaa tcaccttgct cgtgcaggcg
     5101 tcgacctgat gctcgtcgaa tccatgccca cggctcgtga agccctcatc gctacccaag
     5161 ccgccctccg taccgggcta cccgtcatca cctctttgct ggctcgtcgc cacggtttgc
     5221 tttttgacca ctcccccttg cgttccgtca ttgcacaact cgctttgctt cccatccaac
     5281 ttgtcagcgt caattgctgt tccctccagg cttgcgatga agccatcccc tatctatccg
     5341 catgcggtct tccctgggga ttgtacccca acgcctcaga cccagacgga tcgtttggag
     5401 ggacaatcaa ggacctggat cgcgacttat tccgcagcgc cgcgcagtcc tggtttcatc
     5461 gtggagccag catgattggt gggtgttgtg gaacacgacc catgcatctg gcagtgcttg
     5521 actcgcttcg ccatactctg ttgacgccgc agccctcgaa gcctcgagca tgaacgccaa
     5581 gcttgttttg ctaccccagg gtctgtcaaa cctgtcccga cgttgatcga atagcatccc
     5641 cttccccgcc caaacgcgta ctatccggtc gttcacgcat tcattcccat gcccgtatat
     5701 cagggcatct tttccacttc cgcttccgtc gcacgcaaac ttcgatcgcc actcgaggcg
     5761 atataggcca acacgacact cgtggtcatg aacactgctg ctgtcacggc ggtcaaacgc
     5821 gtgagtagat tgccagcacc acgaccacca aatacttggc gagtcgtcga ggcaccgaaa
     5881 gccgcaccaa gaccgccacc cttgccctgt tgcaaaagca cgacgagaat gagaaaaaaa
     5941 caggcaaaga cgtggatgat gctcaggatc gtctcaaaca tgactgcgcg gggttgtacg
     6001 acagccgaag cggttgtgcc acaacgatcg tttcgagccg ccgatcaaga gacagtattg
     6061 aacatccccg tggactgagt tccgttttcc tcgggttcga agacgagcaa aaacgactgg
     6121 agagcagcgg ctagacacat cgaacaggaa aactcttcat agaaaacatg tagttaggag
     6181 ttcgctgggc ccagctatcc ctgccgtagt gtttcgatat ggacgggacc ctattttcga
     6241 attggaaggg gcagctaacc gccactgggg acacgatgat gggtgtcttg ctgctcactg
     6301 gcatttttga atcatgatgc ccacgaacgc gagcctatgt gcggtcgagg catgaacgac
     6361 ctttggcttc gatggtggaa gccagcacct gtcatcccat gccacaacgt gttccgatga
     6421 ttcgtcatgg tcatccccat gcacggatga ccattgactc ttgcaagatc ggtcatccgg
     6481 atccggtctc agtatccgca ttcggatgat tcgaaagccc gaagtacaac agactcgact
     6541 tgcctcattt ggcgagagac aatcggtgcg tccatccatc gggcgtgaca gcaggagccc
     6601 gttctgccag tctcggatgt gctagaaacg agcaggagca ccgtgacccg cataccgcct
     6661 cacgaacggc ccgaaatcga gcgtcccgac gacgtggatg acaaggctgt cctatcggtg
     6721 tttcgggttc cgaaagagtt ggcgggtatg cgcgccgacg tttttttgtc gacgcaatta
     6781 cgaaacacga gccgcacacg cgcgaagatc atcgtgcgag ccgcggctcg atttctcgac
     6841 ggctcccgga tcaaacccaa tacccgcctc gctccggagc aatacatcgc tctgtggcgt
     6901 ccggcttggg acgagacgga aataccattt gacattccca tcttgtttca ggacgagcac
     6961 ctgcttgtca tcgacaaacc tgctggactg ccggtccatc cctccgctcg ttactaccgc
     7021 aataccgtca tcaagaagct tggcgaacgt ttcccttccg agcatctcgt actcgggcat
     7081 cgcctcgatc gagacaccag cggcgttctg gttctcgctc gcaccataga agccgatcgc
     7141 ctgctaaaaa agcagttcag gaacgtcctg ggcgtggaaa agtcctacca ggccatcacg
     7201 tggaatacgc ctccttatcc tcaacagcgc atcgaactac ctctcgaacg aaacccagaa
     7261 tcgcgtctgc gctgcacgat gcgcgtcgct gctcccggaa ccggattgaa agccgccaca
     7321 cgcatcggaa tcatcaaacg ctatcgccgt ggcgatcgtc gatactcgct cgttcgctgc
     7381 gatctcgaaa cgggacgaca acaccagatc cgcgttcatc tcgcctcgct gggatgtccc
     7441 gtggtcggag acaaaatcta tggtcccagt gaagaactcc acgctcgatc ctcggatggt
     7501 acgcttacag acgatgacag gttacaactc gaattgcccc gccatgcttt gcacgcctgg
     7561 cggatggcat tcatgcatcc catgacggac gatcggctcg ttttcgaagc gcctttgcca
     7621 gaggacatgc aggcatttgt tcgtggactc gaagttgacg acaccctttg acgacaacac
     7681 gtgctgtcgt gatcgaagca gggctcaacg cgctgggaaa atcccttgga gacgtgtgcg
     7741 caaggtatgc caatccaggt tcatcagacc gaaatgctct tccagaattt gctttaactc
     7801 attgatttca ttgatttgtt tttcgattct ttgcgatcct cgttgtatcg cgaatcggtc
     7861 acgtctcagg gtaacgcgtc cttgctcggt cgccagcgct gctaccggct cttgggtgaa
     7921 gtacgacatc ggatgggtcg atgtataatg attggctggg atgtagtctt gagcccgaac
     7981 gcaacaaggc agcagtgaat ataggggttt ccagccatgc tctttgagca ccgaaagctg
     8041 aacttcgtga ttgtcggttg gctcgagacg gtaggtatcg agctgctgct gttcgggaga
     8101 aaagaactgc caacgtagcg gcgcacgcaa ggtagctgcc ccaaagccga catcgcacag
     8161 gtactgttgt ccgtccacgg tcacgcgaag cgccatgtgg gtcaaggcgg gagtggtggc
     8221 agatccagac caaacccggc cgagcagccg gtccacctga taacccagcg actcgagcaa
     8281 caggagcaaa aggccattga tctcgaaaca atacccaccc cgtcgttgca tcacgatctt
     8341 ttcgaagatc gcctcggtgg tcaacacgat gggtttgcct tccaaaatcg cgatattttc
     8401 gaaaggcaca tgacgtggaa tggcctgatg caagacgacg agccgctgcc aagggtcgtt
     8461 gggaagagag gaaataccga tacgatcgag aagggccgac caatccacga cggcttacct
     8521 accctccacg gaagtcagtg aaaagaggag aaggagcttt ggtgggaaac gaaaaaacaa
     8581 cgctgatttc tattgtttcg cgatacgtcc gtgcctcgat acgttttccg tcaatcgaaa
     8641 gagtatccat ttttggatag actagagacc ttttggaacg gcattcaaat ccacttgaga
     8701 aagggcatat cgaatcagag cgctgcgatt ggctttggtc aaaccgcgcg ctttgagttc
     8761 atccaccatt tcgtcgagtt ttcccaggtc ctcggtgtac atcgagatac aaatcacttt
     8821 gtaatgggct ggtttttccg gcttggacac cggtttggct cgatgtccac gctgctcggg
     8881 tggcggtgtg tagaacgccc cggaaagtac atcttgtact tcgtcccggt ccaacacact
     8941 tctagccgtc gctcgataat ccccttgttc ctggctcatg ccttcgtcct ccgtcacgcc
     9001 gtgaccgcag ccgctgccat cgttgccggt tccatggtcg ccccagtcgt cacacccaat
     9061 gacgaatccc tgtccatgat gagttgattc actatcgtgc ggtaatcatc cgcagccgtc
     9121 gatgcgcccg catactcgaa gatggtctgt ccatgcgcgg gagcttcttt gactttgatg
     9181 gccgatcgaa tgggaggaag acaccggtcg ccaaaatgct tttgcagcgt ttcgaccgcc
     9241 tcccggcaga tattcgctcg ggcatcatag aaggttggaa gaacacccca cagcttcacg
     9301 gggtgatgca ggagttgatt cacgttgcgc acggttttga tgacttgccg aactcccacg
     9361 agggacaagt agtcacacgc caccggtacg agtacattat ccgcaaatac aagggcgttt
     9421 tggttcatca acgataagct tggggagcag tccaaaacaa tgaagtcata cccgcgaacg
     9481 gccggagaca atcgatcacg caacacgcga tcgcggttct gacgtcccgc gagatacagt
     9541 tccgccgcgg ccaaggtttc attcgacgcg atcaggtcga ggttttcgcg aacggattga
     9601 atgaccgttt tcagcggaag tcccatgacc agagcatggt acaaagactt gtccgcgttt
     9661 ttattcaagg acacgcccac attgccttgc gcatcggtat ccacgagcaa tacgcgccgt
     9721 cctgtctgcg caagcccggc cgcaatactc accgacgtcg tggttttacc cgttccgcct
     9781 ttgtgattga agatggcaaa caccatggct gaacgtttgc ttggggcgtt ttgtacgggg
     9841 ggcgccgtgc gtgcttcgat cgtgggaatc aagctgacag gttctgcggc agaaagcctt
     9901 gcccgattct caggaaggtt ttgatgcgct gcttgagaat atgccgtcgg aggatcgctt
     9961 gccactgacg cgttcgaggc cacaggcatc gtaggcatga tcttcggttc agccgtttcg
    10021 ttcgaaagag gctccgttgg cccgggtggt tgcagcacac cgttggccat atcgccaagc
    10081 cgcacaccac gtgcctcgtc gaggatttgc tctcggcatg gcaaagagca agcatatttc
    10141 cgacgatccg cgacataaag cacttgcgaa acgagttgta cacggaaatg ctttcgacac
    10201 gcatcacacg tcatcaagcc ctgtccgttc gccgtctgac tggtttccag gcatttttga
    10261 gagcaataaa acgaaaaacc gtcttcggat tcctccattt gataacggaa ctgtacctcg
    10321 aaggagttgc cgcacacact gcatgactcg gtcattctgg ccatctgatc gtcctccgcg
    10381 gtcggcattc gtgctcacgc cgccatgtcc cgtcgcttaa tcatgtacag attgaagtgg
    10441 ccaaattttg ccaccgacaa ccagacgtag cgaaccaata catgtatacg aaggtaggat
    10501 gagccaaagc ggctggatgc ggaatgggga gccatgcccc acgcatcaat gccggaaatg
    10561 acgttttcca gtgaagatca tggcgattcc tgcccggttg gccgcatcga tgacgtcttg
    10621 gtccttcacg ctgccgccgg gttgcaccac agcgccaacg ccttgtgccg ccgcaagttc
    10681 gatgccgtcg ggaaagggga aaaatgcatc ggacgccaga actgcgcctc gagaggcctc
    10741 gccagctttt ctacacgcaa tcatgacact ttcgacgcga gacatttgcc ctgctcccac
    10801 gcctaccgtc acgtttggtt tcgcgagtac gatcgcgttg gatttgacgt gtttgacgac
    10861 tttccacgcg aaagaaagcg cgagcatttc ttcttgggtg ggagcccggt tcgtcacgac
    10921 agccgcggct tccacctcgc cttgtgcggt ggtatcgcgt tgttgcacaa caatgccgcc
    10981 gctaatgcga cgaagcacat cggaagggtg gtctggcggg agcatcacac cgcaatcgag
    11041 caagcggagg ttcttttttt tgcggagaat ggcgagggct tcttccgaaa agcctggtgc
    11101 cacgacggct tcgaggaagg tctcggcaag ggcggaagcg gtagcagcgt caacgttgtg
    11161 gttgagtgcg acgataccgc cgaaggcgct cacggaatct gcctctcggg caagtcggta
    11221 ggctttttcg aggctatccg aagtggcgac gccgcatggg ttggtatgtt tgaccacgac
    11281 ggcagccggg gattggaact cccgaacggc ttcgagagcc gcatcgagat cgatgaagtt
    11341 gttgtaggat aattctttgc ctccagcacc caagcttttc gccatggcga tggttccgat
    11401 gggcgcgtgg cgttcgcgga aaaatgcgcc tttttgatga ggattttcgc cgtaacgcaa
    11461 atgataacca cgctcgaagg gcagggttag aaaccttgga aacggctcag gtccttgcgc
    11521 gtcggcatcg gtggaaagat agcgggcgac agcggcatcg taagcggcgg tgtgagcaaa
    11581 tactttggcc gccagttgtc ggcgtttacc aaggtcgagg tctccgtgtt gtagttcttg
    11641 ggctacccaa tcgtagtcgt cggggtcaca gacggcgacg acgcgttgat gatttttggc
    11701 ggcgctacgc aacatcgcag ggcccccgat atcgatattc tcgatgattt ccgcatcgct
    11761 ggcatcggcg cggcttacgg tctgttcgaa cggatagagg ttgacgacca ccatatcgat
    11821 aggtgtggct cctactttgg cgagatcgtt ttcgtcacga cccagtctgg cgagaattcc
    11881 gccatgtacc gcgggatgaa gcgtttttag gcgcccatcc atcacctccg gactaccggt
    11941 gtatcgttct accgggatag cctcgatgcc ttcgtcgacc agcagctttg ccgtgcctcc
    12001 tgtggaaagc aactcgaagc ccagattcga cagggatttg gcgaatggaa cgatacgact
    12061 cttgttgctg acagaaatca aagcgcgacg agtgttcatg ggggggcggg tacccgtatg
    12121 tgcggtcgac gtcaaccgcc ccggcaacaa tcgtgaatga aacgcgatgg atagcgcgct
    12181 gaggaaaacg cgcgagcggg gggaattttc ggctcacgta acgtatcatg agtacgtctg
    12241 ggaccattcg acaccggacc cctttcccga taggaagacc aaccatgcga aatacaagtt
    12301 ggatagttgt tggcttggcg gccattgcct tggccgtcgg ttgcacgacc aagtccgagg
    12361 aggacacggg ccccgccagc gggggttcct caggaggcgg ttatacgcct actcccaacg
    12421 gagtcgggat tggcgagcag gaagcgtgtg agatgctgcg cgacgcgttc gacaagcatg
    12481 cgtcacgact tagctgcacg tatacgttac cggaatgccc agcgtatatt cgggcggggc
    12541 aggctcctgt ttgctccaag tacgacgagg ggacagtgaa gggatgtgct gacttttacg
    12601 ctggattcac gtcttgcagt gagtttcaga agcggccgtg ccatatcatg aatatcgaag
    12661 gcagtgcgcc gacgggttgc cctgatgacg ctggcgtgtc ggacagcgag gagccgacgg
    12721 aggcaggcga gccggatgcc gaggagcagg tggatggnnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12841 nnnnnnnnnn nnnnnnnggg cgagccggat gccgaggagc aggtggatgg gggcgagccg
    12901 gatgccgagg agcaggtgga tgggggcgag ccggatgccg aggagtgacc ggaagcgaaa
    12961 gagcaatgga ataggtacag aagcctgaat caggcaccga agtcgtcgaa actgatggaa
    13021 tccgcgtgat cgagggcgtc ctcactgtct tcgtcgtcat cggtttcttc gactctgtcg
    13081 ggttcctcaa agtcgtcggc ggtcaacatg gggagcctgc gttttccgtg gggtccttca
    13141 tcgacatagt cgcgaagggt ttccatttcc ttgagaagtt gcaattttgc aagggcattg
    13201 acctcgagtt ggcggattcg ttcccgagtc aggttcatga tggctccgac ctgttctaac
    13261 gtggccccgc ccctatcggc cacatcgagg gcgcagcttt cgatcatttc ctcgacttcg
    13321 aggtcgggga aattgagctt gatggcccct gttttgtcag acacgtcgat aaataggtga
    13381 tatcggcaag atacgaacgg gcaaggtcga aggccatcga cgcactcggc ccttgtgcga
    13441 ggtttccaat agtccgtttc gggatacagg agccggccga tctcgagttc tttttttgtc
    13501 attcggcgga cactgatggt acgagcgcgg acatctcgct tccgacggga ccggcgttgt
    13561 tcacgggtca tgggagcggc caaagcgctt tgggttccat cgtttgcggg actcgaagcc
    13621 tgaggcaagg actccatgtt gtcgaaatga ttgttattgt ccatccgagg aatctccagg
    13681 tcgaccatgt acttgtttcg agtttcacac gtacgatcct gcacacggac ggacacctgg
    13741 tccgcacggg ccgaaaccgt ttccatggga gctggggagt cgaagaagag gtaggttaat
    13801 cgtcggacac ggtccgacag acatcccacg gcacctcacc ggtgacaatc cctgcccaat
    13861 gtagcaacac cagggttgct tatttgccaa tcatggccgc tatattctta tcctgtggcg
    13921 gccggcactt ccatgatttc acatcttcgc gcaagatttg tttcgacatt ggcgagccgt
    13981 tccaacaact gatcagccat ggctcgcgcg tgattcaagt ctacggacac cgagtttcgt
    14041 agtgattgtg attccacctt gtcgacaacg gcttccaacg tcacaaatac tttcgctatg
    14101 gctcttcgaa gctcttccaa ctcgcctcgc atgaaaagta cgttttgctg aacttgttca
    14161 atggtgaggc cgtcggaaag caattctttt atttcaatga tttggcgaac gaccgtgacc
    14221 ggatacattc catgggagcc ggtatttttc cctttgatac ccacgcgaac gcttcgcggc
    14281 agcaggccga gttggaccca tttccgcagg gtcgcctcac tgaacttgat acccaaggaa
    14341 gccaataggt cgagaatgtc cgccgaagag atacctagag cgtattcatc ttcgatacgc
    14401 ctcaatacgc tctggtcgat acccccgacg cccacgtcga aacctcccta tcctcacaag
    14461 gaatgacttg gccagatagc tatataagac tacttaacag cgagaacgaa tccccaaaga
    14521 gcgaattgcc ctcgccgctc ggcatccgaa gtcatcaact ccgaatcgac attgaaccta
    14581 tcgcttcagt ggaatgctca caagcctttg gcgaatcatt ttttgagaag cagttgggag
    14641 cgctccatca gaagccggac ggaggtcaac agggacatta tttgtcactg tcagcctccc
    14701 cgtcgatcgc gatttgtctt tggacaatgg acaaattcaa cgtccggctc gggaccatgg
    14761 acacacagct tcgcactcca tctccctgcc taccacacct caattcagta tgttcttatc
    14821 taactcattc ctgaactatg cccgctttcc ccctactatc ctacataaac tgctgcacat
    14881 attttccgca ttgacatgat tgtgttctcg gtgggtgtcg ccgcccccac actctttttc
    14941 tgtttcctgt tcaacatccc tttccttcac tatcctttgc ctcgtgctca tctctcccat
    15001 cgctcgcgac gtcacagctc gaagaattcg tacccgaatc cttcaatccg gatctggttc
    15061 tcccctcgtt ctcattcatg gctttctcag taatcaccac accttcgacg atgtcgttcc
    15121 atcactggcc cgttcatttc aaaccgtaac gatcgacttg cccggaacgg gggaaagtga
    15181 aaaaccacca cccagtcgct tcgattattc catcgaagcg ttcgcggaag tcgtcaccga
    15241 cgtcattgcc gcccttcaaa ttggacgctg tcacgtcgtg ggtcatggtt tgggtgccgc
    15301 catcgccctc gccctcgcct cggagcatcc cgagttcgtc gaccatctga ttttggtatc
    15361 ccctgaaatc tattcgggtc ccccccctcc gctcacccgt atcatgcgta tgccgcttgc
    15421 cggacgcttt ctattcaaac aagtattggg gcgttccttg ttccactctt tgtacctcaa
    15481 gcaccaatac gctcccggac atatcgtcca atccgaacgc atcgactcgt attacggtcc
    15541 cttcatcgaa ccagcggcac gagaaagcgc tttcgccgtc ttacgtgctc aactcgacat
    15601 gcgcccttcc atcgctcggt tatcgcgcat caagtcacgc accctcgttc tttggggtcg
    15661 tgccgatcga cgactcatcg ccgagtacgc acctcgcttg gttcgacaac tccaactcgc
    15721 ccaactcgaa tacatcgaaa ccggccacac gcctcacgaa gaaaaacctg accttttcgt
    15781 agcgtcgctc gagcgattca ttctgggcga acgatcatga ctacctcccc tttgcttcca
    15841 cgattatttc gcagtcgttc cttgatcttg ggttctggtc tcgttgtttt gctcgtgctt
    15901 gtcgccatcg tgggaccttg gttcgtagct catgacccca atcactccga tttcgctacc
    15961 ggccaaggta ccttcggact gccaggaggt ccaaaccggc atcattggct tggaacggat
    16021 acgctatatc gcgacctact ggctcgcctc ttgtacggtg ctcggatctc tctattcatt
    16081 gccttgcttg ctaccaccct ggcagtcacc gtaggaacgc tggtgggcct ttcagcaggt
    16141 ttttttcacg gttcacgcct acgcttcgtg gacacttggc tcatgcgcac ggtcgatgct
    16201 ggcctagcgt ttccctatct gttgctcgtc atggcggtcg gcgctgctgt cgatcgctcc
    16261 gacacgacca ccatcatcat ggttctgggg ctcacgggtt ggcttggaac ctcacggctc
    16321 gttcgtgcga aaaccatttc cgttcggcaa ctcgactatg tcgttgccgc gcgcgccctt
    16381 gggcagaacc ccatcatcat cgccattcgc cacattttac ccaatattgc cggaccgatc
    16441 ctggtcactg cctcggcttc cgtggcttcc atgatccttg cggaaagcgt gctgagctat
    16501 ctatccgtgg gcatccagcc tcccactgcc acgtggggca gaatgctcag ggaaggacag
    16561 acgtttttca ccgcggcacc tcgtctggtt gccgcacccg gtgttgctat cctgttggct
    16621 gtcctggcat tcaatctcat gggggagggc ttccgtgaca cgctcgatcc gcgcactcgc
    16681 taatccgctt gtgttatggc tgtggctgct cggctgccag gacaggttga accagcccca
    16741 gggaaatccc acggaagggc cagcacaaaa cggaggcacc ctcacaatgt cgacgttcgt
    16801 tcacattcga accgtcgacc ccgccgttgc gttcgacgaa gcctccgaac cgatcgttcg
    16861 actcctattt tcccgactgt ttcgcgtgtc acgcacggga caaatcgaag gcgatctcgt
    16921 tgcgcatcat gagctttccc ccaacggtct cgaactcacg atggaattgc gtcgcgatgc
    16981 gcgtttccac gacggttctc ctgtcttggc gggcgatgtg aagcgttctt tcgagcgcgc
    17041 atttcatccg aaaacgccct gtcccgttcc aagttttttt gatcggatcg ttggcttgac
    17101 cgagtatcga aaaggaaaga gcagcgagtt g
//
LOCUS       DAOSOR010000041        17047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_3384_length_17047_cov_3.889889, whole genome shotgun sequence.
ACCESSION   DAOSOR010000041 DAOSOR010000000
VERSION     DAOSOR010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 17047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17047
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3384_length_17047_cov_3.889889"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1441)
                     /locus_tag="PK710_03825"
     CDS             complement(<1..1441)
                     /locus_tag="PK710_03825"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOT08872.1"
                     /translation="MRWSRAFIPTIKEAPADATNASHIYLTRGGFVRRIGAGIYEFLP
                     LGLRVLRKIESIIRQEMDGAGAQELLMPALLPADYFRETGRWDVFGDNLLRLKDRKGG
                     DYHLGPTHEEIITDLVRREVRSYRDLPKNLYQIQTKYRDEPRPRGGLLRCREFVMKDA
                     YSFDVDEAGALASYEAMRGAYIRIFDRLGLTYRMVQADSGSMGGSTSAEFQVLTQSGE
                     DAIGACHLCEYAANLEVASAREPVDQPPSSMAPYQKVHTPNAGTIAEVSAFLKKCPEK
                     FLKSILYQVGGEIVMVIVRGDHEINEVKLAHVLGVGEVLLADDEQIKRATGARVGFAG
                     PVGFQGKIIIDRDAAVVHGGITGANEDDYHFVDVERGRDFQATVADIRSVVQGDPCPH
                     CEKGKIQLFRGIEGGHIFVLGTHYSATMKAHFLDEHGKDKPVVMGCYGIGVSRLVATA
                     VEQHNDEDGILWPMAIAPYQVHVINTGPDE"
     gene            1607..2110
                     /locus_tag="PK710_03830"
     CDS             1607..2110
                     /locus_tag="PK710_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HOT08873.1"
                     /translation="MPRFSQITVIGNAQASAHVLDLAERAGEALGRLGTIITGGRGGI
                     MEAVSRGAKRAGALTIGILPGSDFEDVNPYLDVVLPAGIGFARNMPNVLAGDVVVAIG
                     GASGTLNEMTYAWMFGKPIVALTGAGGWSERLAGQKIDDRRADEILRADSIEQLEKTV
                     RKLLAQP"
     gene            complement(2120..2797)
                     /locus_tag="PK710_03835"
     CDS             complement(2120..2797)
                     /locus_tag="PK710_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08874.1"
                     /translation="MKRNDQWLSFLVSGLMACGGSTPPPQEPIVQPQPPVAEPAPEPE
                     PTDKAEPDKAPEEEAKPERKERRPLATYNDPVEPVTVGFDGAVIRLDGGAEIRIPSGS
                     LASPRNILFTVNKKHKGDQGRLGEVYALQAQVPNQQVQVSNVRASQPLSSNGDPFILK
                     LPIPGQGDSANLAVESVEVDDKGRGKSTWTIVARTKLETSDSGNKAVFEISQLPDAHV
                     HLTTRNP"
     gene            complement(3112..6099)
                     /locus_tag="PK710_03840"
     CDS             complement(3112..6099)
                     /locus_tag="PK710_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT08875.1"
                     /translation="MIAGFGKQTIVLGSAADCDIVLAGPGVAPQHARIVHQGAGQLLF
                     VDGGAGPSFAGGQMVPKGGSVPFDLRTPFACGQVAIPLNHPAIALMLMERGSVQAPPG
                     QIIVGRDPARCSLVIRHASVSSRHATVMLDRMVVVDEGSTSGTYLGSGRIASETPTPV
                     DPNGVIAFGPVVVPVATLGQIVQAMAGVQGQPMVSQAVQAAQASFVSQAMQGQAPMVS
                     QQVPAPVSAGHKKNRTVIGNLDFQKPFQTIGRTPDNDIVLSHPQVSSRHAVLVKSGNQ
                     VFIEDRGSANGTYVKGQRIAPGVKVPVQHGERIYLGPMPLVIHMPEEGGGQLLIPDVS
                     KWQGRPLYEIEAWELFLEVPDRDNPSTNKVLLDKISFKALPGDMIALMGPSGAGKTTL
                     LLALNGYLPPSSGLVRINGENLYAIYDNLRGSIGYVPQDDIVHPELTVFEAVRYSARF
                     RLPPDYSDEEIDQRVDQTLKELGLEGVRNLQIGKPEKKILSGGQRKRVNIALELVTDP
                     VILFLDEPTSGLAADDTTALINLLADLAKATGKTIIMTIHQPAKDEFERFNLALILGY
                     GGVPMFYGPTSPDAYRFFGSFLETQGKPNTVDNPRDMFDILNQREGPIYQQMLAQNPN
                     TPRGAARQYAAKLWEAEYKASAVYQKMYAPPRAVGTGGGQNYVPPGAPKTKGQLRLLM
                     SRYFTVKWRDRSGTAIMFLQAPIIGVLLALVFGGQKDSIPYWCLGALQELSKRAGQLG
                     STGDLLSNMQATRDNSAAAFFVVVAAVWFGTSNAAREIVSERAIYLRERMVNLKLFNY
                     VASKYILLCVFCVLQCTMLLGIVFFSLGFHGGIEAFLWELSYLNAVAFMSAALGLLLS
                     TMVTSSEAAQALTPIALIPQVVLGGLMVPMTTNPLLKWPMLIIPARWGFQGLVAHERK
                     AIAKDPAWLIDIGKPDINSAADFVSAGKFECATAQIAGDNFNGAWGFTDYDLFWVPFA
                     VMGGMTFATLIALLIILKHRDPV"
     gene            complement(6096..7352)
                     /locus_tag="PK710_03845"
     CDS             complement(6096..7352)
                     /locus_tag="PK710_03845"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOT08876.1"
                     /translation="MNQPQRVSLAIASLSDPGLDPEKQVNEDACEARQLPIGTLLVVC
                     DGMGGHVAGKEASEAALRTIFDQMQAASPDAHPGLALKNAIAHAGKVVFDLGGPSAML
                     RPGSTVVAVLSHPAGSEIAHVGDSRAYLIRDSQIHPLTRDHSMVQQMVDAGLLTEEEA
                     KHHPDANKITRALGMSAIVDVEIRPSPLAHQQGDIFLLATDGLCDLVEPHEMLSMTMQ
                     ARQVGSLDQACQQMIALANSRGGHDNTTVLLGEILSCPACSESRSRTLVTDDPIAVTG
                     SAAVAAVPGAMLPTVVDGNDLGVSMPTWVDNGTAQDGRASEAELLQRRKLTWFVVGMA
                     LMVFGIAAVVISVWWAWQATASEEDEVGMREKASFTVVMARSMTERCRECAGTGPDAS
                     GMIAQRRMRSWISDGCPSTDGNGYFT"
     gene            complement(7377..9644)
                     /locus_tag="PK710_03850"
     CDS             complement(7377..9644)
                     /locus_tag="PK710_03850"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT08877.1"
                     /translation="MRIRHLIVAALWASLLLVLTGPVFAAPMARILRIDPIAATTDGA
                     PVLTLVVDLVQSKRVNAVTGECAGNLDCESERLEKPKALFQPFPYPEGNANFLVTIDG
                     SAFPTKYVWKGRWGENQQMPGVGTAWLILVDAGGSMSSRIGDAKAIANAFVNAMGAND
                     IINIMAFDGKQVVKDSKWLSASRKGEASAFVNGIGANYGSWGRTRPLANIIRAGVNDA
                     FNELGNVGSDIVVPMHQALVVISDGAAGTDPSSTGPGALMLTQHLSKGRFPEDNSALP
                     KTPLPVISIWFPRTQIEELAQNAQEFMMNLANPEFGGFYTNVKAGGAGRAPQIVNAVR
                     DRFNHMHIVKYRVSCIASNVQQTFQLVFTNITPMIGGDATFKDVPVGIDPSTWPLDIN
                     VQYTLDRAKKNPVHPGGKFRVYGDFCWSGDKSRAEVYFLPKNQPAPPSVKGGDIETAK
                     RAQQQLIAMNMRGTAKEASDTFVEFEAPNDEKILLGSGDKAVVRMVIYDNKARRASAA
                     DAQHILTLKGSTAPFPFLWVGGGVFGGLVVLLLLVAIVRAGGSKKQRRAGAAPPAPVV
                     AGYGAPQAQPYAGAAAPVAQPYAGAAQPAAQPYAGAAQPGAQPYAGAAQPAAPAANAG
                     FMYGAGQQQGFGLTGAQPQHQAPPPNPYAAPGMGGSKAILSGAAGNFPIPAGSEVFVG
                     RDSARCQVILQELRVSATHAAVRFDGSQVLVRDMGSNNGTSVNGTRIPPNIDTPVPVG
                     SILRFGPVEFMVRVE"
     gene            complement(9674..10123)
                     /locus_tag="PK710_03855"
     CDS             complement(9674..10123)
                     /locus_tag="PK710_03855"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT08878.1"
                     /translation="MAPPGVAGPSAGMYSPPPHPAEPSAGVQGLDPMAETNKAGVGRI
                     LRGFLYSFHTKTEGEFWPLFAGQNSIGRADAGEDLDIFIADPTTSSRHATIISEVPGR
                     FVLQDAGSTNGTFVNDQPVGFQGSVEIHDGDRIRLGGYNAAVRFITR"
     gene            10437..10892
                     /locus_tag="PK710_03860"
     CDS             10437..10892
                     /locus_tag="PK710_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08879.1"
                     /translation="MLAEAANNWAQLPAVPGADKEQVQAPPAADKEQVQAPPGADKEQ
                     VPVRLAAGTAQVPVRLAADTAQVPVRPAADTVQVPVRPAADTVQVLVRLAVPPEQVPA
                     WAHTVPEQLAEEENTRQAVPAWAAAVDQTDQAHLAPWEPHKHRRIYRLD"
     gene            complement(11125..11481)
                     /gene="rplS"
                     /locus_tag="PK710_03865"
     CDS             complement(11125..11481)
                     /gene="rplS"
                     /locus_tag="PK710_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004415194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOT08880.1"
                     /translation="MQTHPKITALEKEQLRHDLSRVMPGATVRVHYRIREGEKERIQV
                     FQGVVIGRRRAGARSSFTVRKISFGVGVERVFAAHSPFISEIEVVNLGIVRRSKLYYL
                     RKLSGKKARIKARREN"
     gene            complement(11866..12987)
                     /locus_tag="PK710_03870"
     CDS             complement(11866..12987)
                     /locus_tag="PK710_03870"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT08881.1"
                     /translation="MMVPQSVGNPTRSAGIFRWLWGIGFVVVTGACGSDAEHDSANHQ
                     GGNHQGDSGGESFCGDRACDEDEDCDTCPSDCGLCGQNSCQQGLRCGKLSCCAVAAVP
                     EGTLMRGRSLLGDDACPCDLTCSKNEEQEHEVSVEPFWLDRLEVTVGRFRQYLRAGAP
                     HPAPQSGAYPPLPETGWDPSWDIHLLAAGTLSDELACHPGFHTWTASPSDMEDRPINC
                     VTWYEAFAFCVWDGGRLPTESEWEMAASGGKNRLYPWGSEAPSPDRAVYDCMVGGVQC
                     TLQDIVPVGSLSRGAGRFSHLDLAGNVLEWAFDWFTMQGYSPALPCTSCVAHAQGTER
                     IVRGGSFAHTQTRLRAAARGGQDPSKRSSYLGFRCARDL"
     gene            complement(12992..13117)
                     /locus_tag="PK710_03875"
     CDS             complement(12992..13117)
                     /locus_tag="PK710_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08882.1"
                     /translation="MKRQRTHEDDGSKAQDSEAARLGSMNRGETPAGLAEMRELF"
     gene            13132..14025
                     /locus_tag="PK710_03880"
     CDS             13132..14025
                     /locus_tag="PK710_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08883.1"
                     /translation="MRNRYAWIVVPMLTLMQGCSAEHDRPNALGASCSSPSCGDANRP
                     DSRPDAKDATSEPTDDVVSSETSDDGSNDGEPYDGPTGSVRGSLFLSNPPAFEITRPS
                     EYSLLFPAEVAVWAFGEEYAAAYDPAEGYHLEGIPTGTWTFVVRDIEEKNGLLQSVFE
                     RQVQEGLVEWDIPVIGKNSFVVIYGSVSPTVIRDPALAQVILSFESCESQGSKRISGV
                     SVSSPLGGMVLVHDGLGWKYDEGSGTGEYGVAVVANLPAESYPGSKVSMSYTFEGATY
                     PIEPFTVFRGGVIRVVVVHPC"
     gene            14240..15046
                     /locus_tag="PK710_03885"
     CDS             14240..15046
                     /locus_tag="PK710_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08884.1"
                     /translation="MLTLGCSSDDKPSGGANPFGTAGSGGTAGSGGTAGAGGSNTGGS
                     VGDAGPDQGPAGQGGISGDAQPDSPVDAEADNAAQDVTLDIVVPVDPCDAPRSAVVGD
                     QLYAGGPTPTDFATAFNAEMNALTVPGPFMVVFDGLDSTQEADWVLKIGTPQGDPPSF
                     DGTPASAPFRLGEKHSIYAQADASFAMRFGTVDIPVVQFSFGGQFDDKCAVFSAEEFS
                     FLIPSSAANIVFHGSTLDDLLGPPTASFDGGKYNAWFVSLSGQMKAVQVP"
     gene            15079..>17047
                     /locus_tag="PK710_03890"
     CDS             15079..>17047
                     /locus_tag="PK710_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOT08885.1"
                     /translation="MTKRTAQLTSFRTPALLLLLGLLTFWGLACSSPSASEDPLNGEP
                     AEQWASSCLDKDGDGFGVGCGNGPDCDDGDPSVTTQCYACTRNATGCPCGVEGKTVAC
                     GELRTSPSGQSKCFMGTKTCSAGVWGPCQQGDSTASMKSPLRPMGLSNNPVSCNNEPC
                     DPYCQDFPDEPDDGMTNPDAGIVGSDAGLQLGLTPVDAGPSTECVTETVEVEPVELDI
                     YLMLDKSGSMDWCPNTTSKCDPYSNSRWSVTTTAIKAFTTSSTANNVGFALDYFPTGS
                     NTCEQTQYATPSVNWTTLTAGASAVATSLGDSANKPDGGTPTLPALRGAITAARARAA
                     STTNRKPIVVLATDGDPNGCGSDINTVSAAAQAGYKGYPLGATVVNEAFVNISSTGTA
                     TKVTGDEATGGPFPIGFSFPYYGGNYSQFWVSTNGFLTVSAAPGGAYYTNGTIPSTSA
                     PNDGVIAPFWDDLRVNGDIYYQTFGTAPNRYLIVQWYNTSHYSNSSASIPMQAVLWEN
                     GDISFRYGSLSGTYSDGSSATIGVENPSGSDGVQHSNNASVLSSNTAIRFHTQPIETY
                     VIGVGLVANLNTIAANGGTGTAYITDSGNSASFIAAMEDIRKRAIGCDYPLPTPADGN
                     LDLDATTVGYRLGSGASTPLDKHKNEDACAGG"
BASE COUNT         3625 a         5120 c         4755 g         3547 t
ORIGIN      
        1 tttcatccgg acccgtgttg atgacgtgga cctgataggg cgcaatcgcc atcggccata
       61 aaatcccatc ctcatcgttg tgctgctcga cagcggtcgc cacgagacga gaaacaccga
      121 ttccataaca ccccatgaca acgggtttgt ccttgccatg ctcgtccaaa aaatgggcct
      181 tcatggtcgc ggaataatgg gtgccaagga cgaaaatatg gccgccttcg atgccgcgga
      241 acagttggat ttttcccttc tcgcagtgcg gacaaggatc gccctgcacc accgaacgaa
      301 tgtcggcgac cgtggcctgg aaatctcgac cccgctcgac atcgacgaaa tggtaatcgt
      361 cttcgttcgc gccagtaata cccccatgaa cgaccgccgc atcgcgatcg atgatgatct
      421 ttccttgaaa accaacaggt cccgcaaatc cgacacgagc gcccgtggca cgcttgatct
      481 gctcgtcgtc cgccaacagg acttccccca cgccaagcac gtgagctaac ttgacttcat
      541 tgatctcatg gtcgccgcga acgatgacca tcacgatctc gccaccgacc tgatacaaaa
      601 tggatttaag gaacttttca gggcattttt tcaagaaagc ggagacctcc gcaatggttc
      661 ccgcgttggg cgtgtgaacc ttttggtacg gcgccatcga cgacggaggc tggtccacgg
      721 gttcacgcgc ggaagctact tccagattcg ccgcgtactc acagagatga caagccccga
      781 tcgcatcttc cccactttgg gtcagcactt gaaattcggc actggtcgac cctcccatcg
      841 aacccgagtc cgcttgcacc atgcgatacg taagcccaag tcgatcgaaa atgcggatgt
      901 aggcgccgcg catggcctcg taacttgcca aggctcccgc ctcgtccaca tcgaacgagt
      961 acgcatcctt catcacgaac tcgcgacagc gcaatagccc cccgcgaggc cgaggctcat
     1021 cgcgatactt ggtctgaatc tgataaagat ttttcgggag atcgcggtaa ctacgcacct
     1081 ctcgacgaac cagatccgtg atgatctctt cgtgcgtggg accaaggtga taatcgccgc
     1141 ctttgcggtc tttgagacgc aacaagttgt cgccaaatac atcccagcgg cccgtttccc
     1201 ggaaataatc cgcgggtagt agagccggca tgagcaattc ctgcgcgcca gccccatcca
     1261 tctcctgccg aatgatgctc tcgatcttac gaagtacacg cagaccaaga ggcagaaatt
     1321 cgtaaatacc cgcacctatc cgacgaacga atccaccgcg cgtcagatag atatggctgg
     1381 cattggtggc atccgcaggg gcttccttga tcgtggggat gaaagctcgt gaccatcgca
     1441 tgggatgcgc ggggtagcac accttccaac gattgtcgcc tgctgatcgg caatcgcgtc
     1501 gtggatgcgg cttgcggctc cacgaacatc aacacggttc cccgcttttt cgcacacacg
     1561 aaaaacggcg ctaccttttt cccacgctct gccataggca gtattcatgc ctcgtttttc
     1621 ccagattacc gtcatcggaa acgcccaagc ttccgcgcat gtcctcgacc ttgccgaacg
     1681 cgcaggagaa gccctcggtc gactcggcac catcatcacc ggcggccgag gcggaatcat
     1741 ggaagctgtc agccgaggcg cgaaacgtgc cggcgccttg accatcggca ttcttcccgg
     1801 aagcgatttc gaagacgtca acccttatct cgatgtggtg cttcccgcag gaattggctt
     1861 cgcacgcaat atgcccaacg tgctggccgg tgatgtggtg gtcgccatcg gcggcgcttc
     1921 cggcacgctg aacgaaatga cctacgcctg gatgttcgga aaacccatcg tggccctgac
     1981 cggcgccggc gggtggagcg aaagactcgc aggacaaaaa atcgatgatc gaagagccga
     2041 tgaaatactg cgagccgatt ccatcgagca actcgagaag accgttagaa aactattggc
     2101 acagccgtga cggcacccat caaggattgc gcgtggtcaa atgtacgtgc gcgtcaggaa
     2161 gctgcgagat ttcgaacacc gccttgttgc ccgaatcgga tgtctcgagc ttggtgcgcg
     2221 ccacgatcgt ccacgtgctc ttgcctcgac ccttgtcatc cacttccaca ctctcgaccg
     2281 ccaagttggc cgagtctcct tgccccggaa tcggtagctt gaggatgaat gggtcgccgt
     2341 tggaggacaa cggttgcgaa gcgcgcacgt tcgatacctg cacctgctgg ttcggtacct
     2401 gcgcttgcaa cgcgtaaacc tcacccaatc gcccctgatc tcccttatgt tttttattga
     2461 ccgtgaacaa gatgttgcgc ggcgatgcca gagaaccgct tggaatccta atctccgcac
     2521 cgccgtcgag acgaatgacc gcgccatcga agccaaccgt aaccggctcg acaggatcgt
     2581 tataggtggc aagaggacgg cgctccttgc gctccggttt ggcttcttct tcaggcgcct
     2641 tgtcaggttc cgctttgtcg gtgggctcag gttcgggcgc tggctccgcg acaggaggtt
     2701 gaggctgaac gatgggttct tgtggcggcg gcgtgctccc accacaagcc atcaagcccg
     2761 aaaccaaaaa gctcaaccat tgatcattgc gcttcatgac ggttctcctt gcagcgattc
     2821 gatgaaagct cgtggctcgc tcgccgttgt gctcccatct atcgggtgct taccatactc
     2881 cattgtggga gaaaaccgca cgtagcgaga agtctcgacg gaaaactacg ttttcatcaa
     2941 agtctgccgc ccctcgggcc cctatctatt tttattccct ggtcggcctc ccccaggacc
     3001 tgaggaatcg aaatatgccc agccaaccat gtgcgccaac gcatggccca tgagcaaaaa
     3061 ccaacgtttc gcgtgggagc gaggggttgg atatgttata acctgatgaa attacacagg
     3121 atctctgtgt ttcaggataa tgagtagcgc gatcagcgtg gcgaatgtca tgcctcccat
     3181 caccgcgaaa ggaacccaga acaggtcgta gtcggtgaag ccccaggcac cgttgaagtt
     3241 gtcgccagcg atttgcgcgg tcgcacattc gaacttgccc gcggaaacga agtcagccgc
     3301 gctgttgatg tccggctttc caatgtcgat gagccaagcg gggtctttcg cgatggcctt
     3361 gcgctcgtgc gccacgagcc cctgaaagcc ccagcgcgcc gggatgatga gcatcggcca
     3421 tttgagcaag ggattggtgg tcatgggaac catgagtccg ccgagaacca cctggggaat
     3481 gagcgcgatg ggcgtgaggg cctgagccgc ctcactggac gtcaccatcg tggaaagtag
     3541 caagcccaag gctgcggaca tgaaagctac ggcgttcaag tagctgagtt cccagaggaa
     3601 agcttcgatc cctccgtgaa atcccaagga gaagaaaacg atgccgagca gcatggtgca
     3661 ttgcaggacg caaaacacgc aaagcaggat gtatttggaa gccacgtagt tgaatagctt
     3721 caggttcacc atcctttccc gcaggtagat ggcgcgctcg cttacgattt cgcgtgcggc
     3781 gttggaggtt ccgaaccata cggcggccac gacgacaaaa aacgcggccg cggagttgtc
     3841 tctcgtggcc tgcatgttgc tgagcaagtc ccccgtggag ccaagctggc cggcgcgttt
     3901 ggaaagctct tgcagcgcgc ccaggcacca ataggggatg gaatccttct gcccaccgaa
     3961 caccagggcg agcagaacgc caatgatggg cgcttgcaaa aacatgatcg ccgtgccgga
     4021 gcgatctctc cacttgaccg tgaagtagcg agacatgagc agacgaagct gccctttggt
     4081 tttcggcgcc cccggtggca cataattctg cccccctccc gtgccaaccg ctcgcggagg
     4141 cgcatacatc ttttggtata ccgcgctcgc tttgtattcc gcttcccata atttggccgc
     4201 gtattgccgt gccgcccccc gcggcgtatt gggattttgc gccagcattt gctggtaaat
     4261 cggtccctcc cgctggttga gaatgtcaaa catatcccgg ggattgtcga ccgtattcgg
     4321 cttgccctgc gtctccaaaa acgatccgaa aaagcgatac gcatccggcg acgtcggacc
     4381 ataaaacatg ggcacacccc cataaccgag gatgagcgca agattgaatc gctcgaactc
     4441 gtctttcgcc ggctggtgaa tcgtcatgat gatcgtcttg cccgtcgcct tcgccaggtc
     4501 cgcaagaaga ttgatcaacg ctgtcgtatc atcggccgca agcccgctgg tgggctcgtc
     4561 gaggaacaag atgaccggat ccgtcaccaa ttccagcgca atgttgacac gctttctctg
     4621 cccccccgac aaaattttct tttccggctt gccgatttga aggttacgaa ccccctcgag
     4681 accaagctct ttgagcgttt gatcgactcg ctgatcgatc tcctcatcgg aataatccgg
     4741 cggtaaccga aagcgagccg agtaacgaac cgcttcgaat accgtcaact ccggatgcac
     4801 gatgtcgtct tgcggcacgt atccgatcga acccctcaag ttgtcgtaga tcgcatagag
     4861 attctcgccg ttgattcgaa cgagacccga cgaagggggc aagtatccgt ttagggctag
     4921 tagtaaggtg gtttttccgg cacccgaggg ccccatgagc gcgatcatgt cgccaggcaa
     4981 tgccttgaat gaaatcttgt ccagcaatac cttattcgtg gatggattat cccgatcggg
     5041 tacctcgaga aataattccc aggcttcgat ttcataaagc ggacggcctt gccacttgct
     5101 gacgtcgggg atgagaagct gccctcctcc ctcctcgggc atgtggatga ccaacggcat
     5161 cggacccagg tagatgcgct caccgtgctg caccggtact ttcacaccag gagcgattct
     5221 ctgccccttc acataggtgc cgttggcact gccacggtct tcgatgaata cctgattgcc
     5281 agatttgacc aataccgcat gacgactcga cacttgcgga tgcgacagca cgatgtcgtt
     5341 gtccggcgtt cgtccaatcg tctgaaaagg tttttgaaag tccaggttgc cgatgaccgt
     5401 acgatttttc ttatgccccg ccgagaccgg cgccggcacc tgctgcgata ccatgggcgc
     5461 ttgcccttgc atcgcctgcg aaacgaagga tgcctgcgcc gcctgaaccg cttgggaaac
     5521 catcggttgc ccttgcaccc ccgccatcgc ctgcacgatc tgtcccaacg tggccaccgg
     5581 taccaccacc ggcccaaacg ctatcacccc gttcgggtcc accggtgttg gcgtttccga
     5641 tgcaatgcga ccggagccca aataggtgcc cgacgtcgac ccctcatcca cgaccaccat
     5701 ccgatcgagc atcaccgtcg catgacgtga ggaaaccgaa gcatgtcgga tgacgagcga
     5761 acagcgcgcc ggatcccgtc caacgatgat ctgtcccggc ggcgcttgca cgctgcctcg
     5821 ctccatcaac atcaacgcaa tcgcgggatg gttgagcggt atcgccacct gaccgcacgc
     5881 aaatggagtt cgaagatcaa agggaacgct gccacctttg ggaaccatct gcccgccggc
     5941 aaaagatggc cctgccccac catcaacgaa caaaagttgc cctgctccct ggtggacgat
     6001 gcgcgcgtgc tgcggcgcca cgcccggccc cgccaacacg atgtcacaat ccgcagcact
     6061 gcccagaaca atcgtctgct ttccaaagcc tgcaatcatg tgaaataccc atttccgtca
     6121 gtcgatggac acccatcgga tatccaggac cgcatccttc gttgggcgat catcccgctt
     6181 gcgtccggcc cggtaccagc gcactcacgg caccgttcgg tcatggaccg cgccatcacg
     6241 acggtaaaac tcgctttttc gcgcataccg acttcatctt cctccgatgc cgtagcctgc
     6301 caggcccacc acaccgaaat gacgaccgcg gcaatcccaa aaaccatcaa cgccattccc
     6361 accacgaacc acgtcagttt tcgccgttga agcagctcgg cttccgaagc ccgcccatcc
     6421 tgagccgtgc cattatccac ccacgttggc atcgagacgc ccaggtcgtt gccatccacg
     6481 accgtgggaa gcatcgcgcc aggcacggcc gccaccgctg cgctgccagt caccgcaatc
     6541 ggatcatcgg taacgagggt gcgcgaacga gattccgaac acgcgggaca actcaggatt
     6601 tcaccaagca ataccgtggt gttgtcatga cctccccgcg agttcgcaag ggctatcatc
     6661 tgctgacacg cctggtccag actccccacc tgacgagctt gcatcgtcat gctcaacatc
     6721 tcgtgaggct ccaccagatc gcataagccg tcggtggcca atagaaaaat atctccttgc
     6781 tgatgagcga gaggagaagg acggatttcg acatccacga tcgcactcat tccaagcgcc
     6841 cgtgtgattt tattggcatc cgggtggtgt ttcgcttcct cctccgtgag caatccggcg
     6901 tccaccattt gctgcaccat cgaatggtcc cgggtcagag gatggatctg cgaatcccga
     6961 atcaagtaag cacggctatc tcccacatgg gcgatttccg acccagcggg atgagataac
     7021 accgcgacta ccgtcgagcc aggacgcaac atcgccgagg ggccgcccag gtcgaagaca
     7081 actttccccg cgtgagcaat ggcgttttta agcgcaagcc caggatgagc gtcaggactt
     7141 gcggcttgca tttggtcgaa aatcgttcgt agagccgctt cgctggcttc ctttccagcc
     7201 acgtgccccc ccatcccatc acaaacgacg agcagtgtcc cgatgggaag ttggcgagct
     7261 tcgcaagcgt cttcgttgac ctgcttttct ggatcgagtc caggatcgga caaagatgcg
     7321 attgctaacg aaacccgttg tggttgattc acgggcatga ccatggcccg tcgagactac
     7381 tctactcgaa ccataaactc gacgggtccg aagcgaagaa tggatcccac gggaaccggg
     7441 gtatcgatat tcgggggaat tctagtgcca ttgaccgagg tgccgttgtt gcttcccata
     7501 tcccgcacca gcacctgact accgtcgaaa cggacggccg cgtgggtggc cgaaacgcga
     7561 agttcctgca aaatgacttg gcaacgcgcg gaatcccgac ccacgaacac ttcggaaccg
     7621 gcagggattg gaaagttgcc cgccgctccc gaaaggatgg ctttggagcc acccatgccc
     7681 ggtgccgcat agggattggg aggcggagcc tggtgctggg gttgggctcc cgtaagccca
     7741 aaaccttgtt gttgcccggc accgtacatg aaaccggcat tggcagccgg ggccgccggt
     7801 tgagccgccc ccgcataagg ctgcgctcca ggttgagccg cccccgcata aggttgggcc
     7861 gccggttgag ccgcccccgc gtaaggttgg gctaccggtg cagccgctcc agcgtagggt
     7921 tgcgcttgcg gtgcgccata tcccgccacc accggagctg gcggcgccgc cccggctcgt
     7981 cgctgttttt tacttcctcc cgctcgcacg atcgccacga gcaacaacaa caccacgagg
     8041 cctccaaaca ctccgcctcc tacccatagg aaaggaaacg gcgcggtgga gcctttgagc
     8101 gtgaggatat gctgcgcatc cgcggcgctc gctcgacgcg ccttgttgtc gtaaatgacc
     8161 atgcgtacga ccgctttatc accgctgccc agcagaattt tttcatcgtt cggagcttcg
     8221 aattccacga acgtgtccga agcctctttg gccgtgccgc gcatgttcat cgcaatgagc
     8281 tgctgctgcg cgcgttttgc ggtttcgatg tcaccacctt tgacactcgg tggcgcgggt
     8341 tgatttttcg gcaagaaata gacttccgcg cgggacttgt ccccactcca acagaagtca
     8401 ccatacaccc gaaattttcc tcccggatgc acgggattct ttttcgcccg gtcgagcgtg
     8461 tactgcacgt tgatgtccaa cggccacgtg ctcggatcga tgcccaccgg aacgtccttg
     8521 aaggtggcat ctcccccaat catcggcgtg atgttggtaa atacgagctg aaaagtctgc
     8581 tgcacgttgg aggcaatgca tgacacccgg tacttgacga tatgcatgtg gttgaaacga
     8641 tcgcgcacgg cattgacgat ctgcggcgcc cgtcccgctc cccccgcctt cacgttcgta
     8701 taaaagccgc caaactccgg gttggcgaga ttcatcatga actcttgcgc gttttgcgcg
     8761 agttcttcaa tttgcgtacg cggaaaccaa atcgaaataa caggaagcgg cgttttcgga
     8821 agcgcgctgt tgtcctccgg aaacctgcct ttgctcagat gctgcgtgag catcaatgcc
     8881 ccaggccccg tcgaagatgg atcggttccc gccgcgccat cggaaatgac cacgagagcc
     8941 tgatgcatcg gcacgacgat gtcactgccc acgttgccaa gctcgttgaa ggcgtcgttc
     9001 acacccgcgc gaatgatgtt ggcaagcgga cgcgttcgtc cccaactccc gtaattggct
     9061 ccaatgccat tgacaaaagc actcgcctcg cctttgcggc tcgcggataa ccacttggaa
     9121 tccttgacga cttgcttacc atcgaaggcc atgatgttga tgatgtcgtt ggcacccatg
     9181 gcattgacga acgcattggc aatggccttc gcatcgccaa ttcgcgaaga catcgagccg
     9241 cccgcatcga caaggatgag ccacgcggtc ccaacccccg gcatttgctg attttctccc
     9301 caacgtcctt tccatacgta cttggtagga aaggccgaac catcgatggt cacgagaaag
     9361 ttggcgttgc cttccgggta gggaaatggc tgaaagagcg ctttgggttt ctcgagtctc
     9421 tcggactcgc aatccagatt gcccgcacac tcaccggtga ccgcgttgac gcgtttggat
     9481 tgcaccaagt cgaccacgag ggtgagaacg ggtgcgccat cggtcgttgc cgctatcgga
     9541 tcgatgcgaa ggatacgggc catgggggcc gcgaaaaccg gtccggtcag aaccagcaac
     9601 agagacgccc aaagggcagc aacgatgaga tgtcgtattc gcatggactg tgttctccgc
     9661 ggtcatccag aagctatcgt gtgatgaatc gaactgccgc gttgtacccg ccgagacgga
     9721 ttcgatcacc atcgtgaatc tcgacggatc cttggaaacc gacaggctgg tcgttgacga
     9781 acgttccgtt ggtcgaacct gcatcttgca gaacaaaccg accgggaacc tccgaaatga
     9841 tcgtggcatg ccgtgaggat gtggtgggat ccgcgatgaa aatatccaga tcctcacccg
     9901 cgtccgcacg tccaatggag ttctgcccag cgaaaagcgg ccaaaactca ccctcggttt
     9961 tggtgtggaa gctatacaga aatcctcgca gaatcctgcc gacacccgct ttgttggttt
    10021 ctgccatcgg atcgagcccc tgcacacccg cggacggttc cgccggatgg ggcggcggac
    10081 tatacatgcc cgcggaagga cctgcgacgc ccgggggagc catggccggg gcctcgacgg
    10141 cgaagggaaa ggactcatcg cgccggtggg aggcaaagga gccggtcccc ccggggaaaa
    10201 ggcagtggct tcgaatgctt cgcttcccgc gatgcgcata tccgccagcg ccggaggtga
    10261 accatggccc ggcccgtggt tcggtggagt gcccgtaaca ccataggggt cggcgggcag
    10321 cgcggcaccg ggggaaggat atccccctaa atccatgggc tggggaggtg aatacggagg
    10381 catgcctggc accggttggg gtgcataagg accaggctcc ggctgtgggt tgaacggtgc
    10441 tggctgaggc ggcgaataat tgggcgcagc tcccggcggt gcctggggcg gataaggagc
    10501 aggtccaggc accccctgcg gcggataagg agcaggtcca ggcaccccct ggggcggata
    10561 aggagcaggt cccggtacgg cttgcggcgg gtacggcgca ggtcccggta cggcttgcgg
    10621 cggatacggc gcaggtcccg gtacgccctg cggcggatac ggtgcaggtc ccggtacgcc
    10681 ctgcggcaga tacggtgcag gtcctggtac gccttgcggt gccgccggag caggtcccgg
    10741 catgggcgca tacggtgccg gagcaactgg ctgaggagga gaatacgcgg caggcggtcc
    10801 cggcatgggc tgcggcggtt gaccagacag accaggcgca ccttgcgcca tgggagccgc
    10861 acaaacaccg cagaatttat cgtttggatt gattggtgcg ccacagcgcc aacacacact
    10921 catcagctta tcctcttggt agaatcgaca cccgcaaagg gtggtcgctt tcgcatggta
    10981 acctgtcagg aaaatcgatg cgagtagttg gaaagggaga atcgcatccg aaggataagc
    11041 gctgagcata caccaacaca catgctccag ggcaagagca agggtgccaa ggcgtgagcc
    11101 aaggcaaggg tgagcgcgaa acgcctagtt ttcgcgtcga gccttgatac gggctttctt
    11161 tccggacagc ttgcgcaggt agtatagctt ggaacgccgg acgatcccca ggttcaccac
    11221 ctcgatttcg gagatgaaag ggctgtgggc ggcaaatacg cgttcgacac cgactccgaa
    11281 actaattttt cgcacggtaa aggagctgcg ggcacccgcg cgacgccgtc cgatgacgac
    11341 cccttggaat acctggatgc gttctttttc gccttcgcgg attcggtaat ggacgcggac
    11401 ggttgcgccg ggcatcacac gggacaagtc gtggcgaagc tgctcttttt cgagagccgt
    11461 gatcttggga tgggtctgca tgacacgtct tcctgcaaac gaaagggagc gcattatgca
    11521 cagatacgag tatccgtcaa cgggggtttt gcaggttcgt gcaagagagc gggcggctgc
    11581 ggcttccgaa tcgatggacg accccacttc cgattttcgc tgagcgcagt gccgctctcc
    11641 caggacgact tttcatgact gcatctcgag cgttggtcat gcctcgccgc ggcaccaaag
    11701 catttttcaa accatgatcc aattctgatt ctacttattt gtataggatg acaaccccaa
    11761 aaacggattt tgtaatcggt aaattgatca tataattcaa tatgttatca ttgatcagtt
    11821 gggtgtgact atacgaatat ggtttcggtg gctagcgcgt cgtgcctaca ggtcgcgagc
    11881 gcaacgaaaa cccaaatagc tgcttcgctt cgacgggtcc tggcccccgc gggccgccgc
    11941 tcgcaaacgg gtttgggtat gcgcaaagct tcccccacga acgatgcgtt cggttccctg
    12001 cgcgtgtgcc acgcaggaag tacacggcaa cgcgggagaa tacccctgca tggtgaacca
    12061 gtcgaaagcc cactcgagca cgttgccggc aagatccaga tgcgaaaaac gcccggctcc
    12121 tcgcgacaag ctgcccaccg gcacaatatc ctgcagcgtg cattgaacac cgccgaccat
    12181 gcaatcgtag acggcccggt ccggcgaagg agcctcactg ccccagggat acaagcgatt
    12241 cttccctccc gatgccgcca tttcccattc cgattccgtc ggcaatcgac cgccatccca
    12301 cacgcaaaat gcaaacgcct cgtaccacgt gacgcaattg atcggtcgat cctccatgtc
    12361 cgacggcgat gcggtccacg tatggaatcc aggatgacag gccagctcgt cggataaggt
    12421 gccggcagca agcaaatgga tatcccacga agggtcccaa cccgtttcgg gcaagggggg
    12481 ataagccccg ctttgcggcg cgggatgggg cgctcccgct cgcagatact gtcgaaaacg
    12541 tcccaccgtg acttccagcc tgtccagcca gaatggttca acactcactt cgtgttcttg
    12601 ttcctcgttc ttggaacatg tgagatcaca cgggcaagca tcatccccta acagacttcg
    12661 tccacgcatc aacgttcctt ccggaaccgc ggcgacagca cagcaactca acttgccaca
    12721 tcgcagccct tgctgacacg agttttgccc acaaagcccg caatccgacg ggcacgtatc
    12781 gcagtcttca tcctcgtcac aggcccgatc gccacagaat gattccccac cggaatcgcc
    12841 ttgatgattt cctccctggt gattcgctga atcatgttcg gcatccgaac cgcaagctcc
    12901 ggtgaccacg acgaatccaa tcccccataa ccaacggaaa atacctgctg agcgcgtagg
    12961 gtttccaacg gactgcggaa ccatcatgag cctagaatag ctcccgcatc tccgccaagc
    13021 cagcaggtgt ctcaccccga ttcatcgaac cgagcctggc cgcctcagaa tcttgtgctt
    13081 tggacccatc gtcttcgtgc gtacgttgac gtttcatgac agaatcgcgg catgcggaac
    13141 cgttacgcct ggatagtcgt gcccatgctc accctcatgc aagggtgcag tgccgagcac
    13201 gatcgtccca acgccctggg agcatcctgc tccagccctt cttgcggaga cgcaaaccgt
    13261 ccggattcgc gaccggacgc caaagacgcc acctctgagc ccaccgacga tgtcgtttct
    13321 tctgaaacga gtgacgatgg aagcaacgac ggtgagccgt atgacgggcc cacgggaagc
    13381 gttcgcggtt ctctgttcct atcgaatcca cccgccttcg aaatcacccg gccaagcgaa
    13441 tactctctgc tttttccagc cgaggtcgcc gtatgggcgt tcggtgaaga atacgccgca
    13501 gcctacgatc ctgccgaagg ctaccatctc gaagggatac ccacgggaac atggactttt
    13561 gtcgtgcgtg atatcgaaga gaaaaacggt ttgctgcaaa gcgtattcga gcgacaagtg
    13621 caagaagggc tcgtggaatg ggatatcccc gtcataggga aaaattcctt tgtcgtcatc
    13681 tacggctctg tctctccgac ggtgatccgg gatccggcgt tggcacaggt catcctgagc
    13741 ttcgaatctt gcgagagcca aggaagcaaa cggatttctg gtgtttccgt ttcctccccc
    13801 ctcggtggaa tggtactcgt gcatgatgga ttagggtgga aatacgatga gggtagtggg
    13861 accggcgaat acggtgtggc ggtcgtggcc aaccttcccg cggagtcgta tccgggttcg
    13921 aaagtgtcta tgtcgtatac ttttgagggt gccacttacc ccattgagcc tttcaccgtg
    13981 tttcgtggcg gcgtcattcg ggtggtcgtg gtccatcctt gctgaactca tatcgttgtg
    14041 tccgtatatg aaaaaagatc gatccctcgc actctttgcg gacagctagc cgctccctta
    14101 tcgacaagca gcgaggcatt ccttcagatg gatcagagat tttcggtccg aattgtgcag
    14161 gcatgaagac gtgccctgta ttttcgaaga agggaatccg atgcgagtgt atggttgcat
    14221 cctagcttgg ggtatactgg tgctgacgtt gggttgctcg agcgacgata agccttcggg
    14281 cggcgcgaat cctttcggga ccgcaggctc tggtggaacc gcaggctctg gtggaaccgc
    14341 gggagccggt ggttccaata cgggcggaag cgtcggtgat gcaggtcccg accaaggccc
    14401 cgcaggacag gggggaatat cgggtgacgc gcagccggat tcacccgtgg atgccgaagc
    14461 tgacaatgcg gcccaagatg ttacgctgga tatcgttgtt cccgtggatc cttgcgatgc
    14521 ccctcggtcc gcggtcgtgg gtgatcaatt atacgctgga ggacctaccc ccacggattt
    14581 tgctaccgcc ttcaatgccg aaatgaatgc gctgaccgtt cccgggccct tcatggttgt
    14641 tttcgatggc ctggactcga cccaagaagc ggattgggtc ctgaaaatcg gcacccccca
    14701 gggcgatcct ccctctttcg atggcacgcc cgcttctgct cccttccgcc ttggggaaaa
    14761 acactccatc tatgcccaag ccgatgcctc gtttgccatg cgtttcggca ccgtggatat
    14821 tcctgtcgtt caattctcct tcggcggtca attcgacgat aagtgtgccg tattttccgc
    14881 cgaagaattc tcgtttctga tcccctcatc ggctgccaac atcgtatttc acggttctac
    14941 cttggatgac cttctcggtc ctcctactgc ctcgtttgat ggtgggaaat acaacgcgtg
    15001 gtttgtttct ttgagtggac agatgaaagc ggtgcaggtg ccatgatgac gatacgcccg
    15061 tcgtttcgga ggtggctcgt gaccaagcga acagcccagc taacatcgtt tcggacccct
    15121 gcgctgctct tgctgctcgg gctgcttact ttctgggggc tggcgtgctc gtcgccgtcc
    15181 gcttcggaag atcccctcaa cggtgaacca gcggagcaat gggcctcgag ttgcctggac
    15241 aaggatggcg atggctttgg cgttgggtgt ggaaatgggc ccgactgcga cgacggcgac
    15301 ccgtctgtga ccacccaatg ctacgcttgc acgcgcaatg ctaccggctg cccctgcggt
    15361 gtggaaggca agacggtggc ttgtggcgaa cttcgtacct cgccatccgg gcaatcgaag
    15421 tgtttcatgg gaacgaaaac gtgttccgcg ggcgtatggg gcccgtgcca gcagggggat
    15481 tccaccgcga gcatgaaatc ccctcttcgg cccatgggct tgagcaataa cccggtgagt
    15541 tgcaacaacg aaccctgtga cccctactgc caggacttcc ccgacgagcc agatgacggc
    15601 atgacaaatc cggatgcggg tatcgtgggc agtgatgccg gactgcaact cggtctcaca
    15661 cctgtggacg cagggccgtc gacggaatgc gtgacggaaa cggtcgaagt cgagccggtc
    15721 gaactcgata tttacttgat gctcgataag tccgggtcga tggattggtg cccgaataca
    15781 acttcaaaat gtgatcccta ttccaattca cggtggtcgg tcaccaccac ggcgatcaaa
    15841 gcgtttacca ccagttcgac ggctaacaat gttggctttg cgttggacta cttccctacg
    15901 ggttcaaaca cctgtgaaca gacccaatac gccactcctt cggtgaactg gactacattg
    15961 accgcggggg cttccgcggt tgcgacttcc ctaggagact ccgccaataa acccgatgga
    16021 gggacgccca cgcttcccgc actccgggga gccattaccg cggctcgtgc tcgggcggcc
    16081 agcaccacaa atcgaaaacc gattgtcgta ttggccaccg atggtgatcc caatggatgc
    16141 ggctccgata ttaacacggt ttctgctgcg gcgcaagctg gatacaaggg ttatcctctt
    16201 ggcgctacgg tcgtcaacga ggcttttgtg aatattagtt cgacaggaac tgccacgaag
    16261 gtgacgggcg acgaagccac gggcggcccc ttccccattg gattttcctt tccctattac
    16321 ggaggaaatt attcccagtt ttgggtgagc accaacgggt ttttgacggt gagcgcggct
    16381 ccagggggcg catattatac gaacggaacc atcccaagca ccagcgcccc taacgatggt
    16441 gtgatcgccc cattttggga tgacttgagg gtcaacgggg atatttatta tcagacgttt
    16501 ggaacggctc ccaaccgtta tctcatcgtg cagtggtaca atacatccca ctatagcaac
    16561 tcgagcgcaa gtatcccaat gcaagctgtg ctttgggaga atggggatat atctttccgc
    16621 tatggatcac ttagcggtac ttactccgat ggctcttccg ccacgatcgg agtcgagaac
    16681 ccttccggat cggatggcgt tcaacattcg aacaacgcga gtgtattatc cagcaacaca
    16741 gccattcgat tccacaccca acccatcgaa acctacgtga ttggtgtggg attggtggcc
    16801 aatttgaata ccattgcggc caatggcgga acgggtaccg cctatatcac cgattcgggt
    16861 aattctgcct cgttcattgc cgccatggag gatatccgta aacgggcaat tggttgcgat
    16921 tacccgcttc ctactccggc cgacggtaac ctggatctgg acgcgactac ggtggggtac
    16981 cgtctcggtt cgggagcttc caccccgttg gacaagcata agaatgagga cgcctgtgcc
    17041 ggagggc
//
LOCUS       DAOSOR010000042        17036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_3386_length_17036_cov_4.863200, whole genome shotgun sequence.
ACCESSION   DAOSOR010000042 DAOSOR010000000
VERSION     DAOSOR010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 17036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17036
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3386_length_17036_cov_4.863200"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="PK710_03895"
     CDS             <1..1195
                     /locus_tag="PK710_03895"
                     /inference="COORDINATES: protein motif:HMM:NF017730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HOT08886.1"
                     /translation="VDVMGLDDWLERWNPSILLRISDEREIPISFSSLDDFHPDALFD
                     RLPIFAELRLLQQEARQGKGIVGQGECGSRESTQDTLARLLGKPNAGGASSSPLQDFF
                     HRLVEPHVVTSETDGMNAQLEVLHEASGELMHTLLHHSAFQRLESIWRGLDFLLRFGE
                     GFDVSVSNLSREELEAWVLVDDPLATPLGRRLLGEAAGEPAWSLVLGAYSFGRNARDV
                     MLLRRLSSILAEAGVPMIASYRASEGGEAEVPSSWRELRSSREASMIGLALPRFLLRL
                     PYGKRTDEISRFPWEEQTTPPQHDRYLWGHPGWLVACLLGRRVIDSGTKPSLSMSGLP
                     VHSFLGEGQMEQTPCAEWWLTEQEAGRWLDDGMIPLCSVRGRDEVRLIRVQSLAAPSA
                     GLAGF"
     gene            1287..2219
                     /locus_tag="PK710_03900"
     CDS             1287..2219
                     /locus_tag="PK710_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOT08887.1"
                     /translation="MQPCESGVEYAEIDTFFGQRGAGYAGATIRGIDLCRSDLSGLDF
                     GGAKFVGVDLTDANLSRCNLRGAIFSNSECGGIDVSGANLQGVRFEESRLSTRKIGGA
                     DLQGAAFVQADLTEADFRQVKVVGARFERCLLTRIQAEGVDFRGCILNDSTLSKARLK
                     GARFDGVCASQACFENADLTKAVLIRVSAPFARFAGALLEEANADDADLTHASFLRVK
                     AQRALFRRAKLTRTTWQAADVRGVRFDMSELNYADFSHALADLASFQKAVLDHANLHR
                     TSLASSILSGASMNGVRKTDPDRARAEDFTTGRR"
     gene            2222..2800
                     /locus_tag="PK710_03905"
     CDS             2222..2800
                     /locus_tag="PK710_03905"
                     /inference="COORDINATES: protein motif:HMM:NF023482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3540 domain-containing protein"
                     /protein_id="HOT08888.1"
                     /translation="MTRQAALRLYPDPKESERVHTPRGASATAEGERLVVRDARGAIV
                     VVYDAEAGSATIVAPVGDLRLAAPTGSVVIEAGEDVELSSRRTVRTRAVAVESDADVT
                     RFRSKAFEVVTGVWQTTARTVVHGVGSWSLGAERVLERANDVVRAVQGLMETRAGRVR
                     TVVQDTTQVRSGSTSISSKEDTFIDGRRVLLG"
     gene            2811..3236
                     /locus_tag="PK710_03910"
     CDS             2811..3236
                     /locus_tag="PK710_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4150 domain-containing protein"
                     /protein_id="HOT08889.1"
                     /translation="MAGVVFPAATMKGGQAMCLGPIDVCKVPAPPAPPIPMPFPNIAM
                     LNMALNTTTTVLFENMPVLVQMSETPLSSGDEPGVAGGVISGVFIGPMMCKVGSGKVK
                     AQGKGVMVMTGMTSHNGKNPNVPVGMIVQAANTKVLVSP"
     gene            3845..4891
                     /gene="tssA"
                     /locus_tag="PK710_03915"
     CDS             3845..4891
                     /gene="tssA"
                     /locus_tag="PK710_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HOT08890.1"
                     /translation="MIDVTKVLEPVTDGAPCGEDLEYDEAFVELQLVAQYKPEQRMGE
                     SLIPAEEPVWRDVESKASVLFERTKDLRVAVHLLKALLHTGGFVEAAKGMQVLRALVI
                     EHWQCVHPQLDPDDDDDPTMRFNILADLADASTMLTWVRKIPLVTVRGIGSFGLRDIA
                     LASGQVVAVEGEVAPSMALIDGAFHEASQEELEPVGAAAASCVEALRSIEKELMARAQ
                     GASPPNLAPLRELFEQAAYAVQSRLADRGHGMAGVDLGSAGEGQVMAAGGPAIKRLSG
                     EITSREDVIKAIDKICEYYAKNEPASPLPLLLGRCRRLVNKDFMGIIRDMAPDGLSQV
                     KVLRGPVEEEDDDS"
     gene            4888..5391
                     /gene="tssB"
                     /locus_tag="PK710_03920"
     CDS             4888..5391
                     /gene="tssB"
                     /locus_tag="PK710_03920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HOT08891.1"
                     /translation="MSGQKFVGRNRAPRVQIEYDVELYGAEKKVQLPFVMGVMADLSG
                     NPAEPLPKVADRKFLEVSVDNFDDRMKAMKPRVAFQVPNTLTGEGNLNIDLTFESMDD
                     FTPAAIARKVEALSKLLQARQQLSNLVTYMDGKTGAEELIARVMADPTLLQALASAPK
                     DGEGQGG"
     gene            5394..6887
                     /gene="tssC"
                     /locus_tag="PK710_03925"
     CDS             5394..6887
                     /gene="tssC"
                     /locus_tag="PK710_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HOT08892.1"
                     /translation="MADSETVKSEGQLQTLEVSDFESLLQKEFRPQTDNARQEVQNAV
                     RTLAEQALQGSVTVSDDVVQTIKQIIAEIDKKLAEQINVILHHADFQKLEGAWRGLHY
                     LVNNTETDEMLKIRVLNVSKKDLHKTLKKFKGAAWDQSPIFKAIYEEEYGKFGGEPYG
                     CLVGDYYFDHSAPDVELLGEMAKISAAAHAPFITGTASSLFQMQSWSELANPRDLSKI
                     FETPEYAAWKSLRESEDSKYLGLALPRFLARRPYGAKTNPVEGFDFEEQISGSAEDQF
                     TWSNSAYAMAVNINRSFKLYGWCSRIRGIESGGAVGGLATYTFPTDDGGVEMACPTEI
                     GISDRREAELAKNGFMPLVHKKNTDFAAFIGAQSLHKPAVYDDPDASANAALAARLPY
                     LFATCRFAHYLKCIVRDKVGSFKERDDMKRWLQDWVIQYVDGDPAHSSEEVKAQKPLA
                     AAEVEVQELEGNPGFYSAQFFLRPHYQLEGLTVSLRLVSKLPSAKGG"
     gene            6997..7482
                     /locus_tag="PK710_03930"
     CDS             6997..7482
                     /locus_tag="PK710_03930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HOT08893.1"
                     /translation="MAVDMFIKIGDIKGESMDDKHKDEIDVLAWSWGGSQSGSLHTGS
                     GGGAGKVSIQDLTLTKWLDKSSPVLFQTMCEGKHIKETVLTVRKAGGTPVEYLKITLI
                     DAIVTSVSLGGSGGEERLTENVSLNFAKFKLDYVPQKEDGSAMPAVQMAWNIAANTPK
                     V"
     gene            7634..8452
                     /locus_tag="PK710_03935"
     CDS             7634..8452
                     /locus_tag="PK710_03935"
                     /inference="COORDINATES: protein motif:HMM:NF018699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HOT08894.1"
                     /translation="MLAEDKLRAGQVDEALALLQDEVRKSPGNVRYRVFLFQLLSVLG
                     QWDRAWLQLKTSGDLDPQCLPMVQTYRELLRCEVFRSHVFAGSKTPVFLGEPQPWAAL
                     LVQALRRTAEGKHDDAQELRSQAFDSAPTTSGQVDGKRFSWIADGDVRLGPVLEMIVH
                     GRYTWLPFHQVRSLRIEPPKDLRDLVWMPVILTLVTGGELVAFIPSRYPGAESHSDAA
                     VRLARRTDWTEIPGGYLGAGQRVWTTDEGEHALFDVREVDLDVDVVDENVVTDG"
     gene            8445..8963
                     /gene="tssE"
                     /locus_tag="PK710_03940"
     CDS             8445..8963
                     /gene="tssE"
                     /locus_tag="PK710_03940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HOT08895.1"
                     /translation="MAELTYRERLQPSLLDRLTDDQRDQSQESRLDRVISPSRLRECV
                     RRDLVWLFNTTNLSSLKSEINEYPLVAQSTLNYGLPDLAGHTVMSIDVVSIERLLRNA
                     IWSFEPRLIRNSVQVRLDVALDEMTHNAMTFTIEAELWAQPLPLRLYFKTGIDLETGE
                     ATVEEVGKGQRG"
     gene            8965..10824
                     /gene="tssF"
                     /locus_tag="PK710_03945"
     CDS             8965..10824
                     /gene="tssF"
                     /locus_tag="PK710_03945"
                     /inference="COORDINATES: protein motif:HMM:NF017733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HOT08896.1"
                     /translation="MDPRFLGYYNRELQFIREMGGEFAREYPKIAGRLGMDGFDVADP
                     YVERLLEGFAFLTARLQLKIDDEFPRFTQHLLEMVYPHYLAPTPSMAVVQFHPSLKEG
                     ALERGVTIARGTPMRSLIGKDEQTACEYRTAHDVVLWPIEIVGFEYRANLGDVLGLGE
                     RIRHAQAGARLRLRTTLGMTFDKLALEELVLFLRGEWEVSTRLYEALVGHTVAVFARP
                     VGEGRWVALGRGAVQALGFDDDHALLPFGPQSFQGYRLLQEYYAFPQRFLFVQLAGLG
                     PVVRACTTQELEIVILFDSKDSLLDNAIEPNLLALHCAPAINLFPRRADRIHLSNQAR
                     EYHVVPDQTRPMDFEVYSVMEVVGHGQAVQQRFFPFYECNEATAGEQDTFFTLHRMPR
                     RLSDKQRRTGPRSTYVGSEVFISLVDGEQGPFRSSLKQLAIETLCTNRDLALHMPLGQ
                     GSTDFSLQTGAPVKAIRCVAGPTQPRPSHAHGEMAWRLISHLSLNYLSLTDGPAGKGA
                     AALRELLVLYADQEQAALRREIDSVLSVSSTPVIRHLPVDGMMAYGRGIEITLTCDET
                     GFLGAGVFLLGAVLERFFARYVSINAFTETVLRTVQRGEVMRWQPRIGTTQAL"
     gene            10788..11801
                     /gene="tssG"
                     /locus_tag="PK710_03950"
     CDS             10788..11801
                     /gene="tssG"
                     /locus_tag="PK710_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HOT08897.1"
                     /translation="MAAADRNNPGALALLSELESHPESFDLFLALRRIECVFRQNARL
                     GEGIHPSDEPVRLAQEPSLAFTSNTLSGVRVRGSIPRVRTRILGLLGPFGPLPLHLTE
                     YAYDRSRNRNDPTFAHFLDLFHHRILSLYYRAWANTEPTVAFDRPQTARFTRYIASLI
                     GMGMPSFLCRDELPDHVRFFFAGRYAAHSRNADGLAALLHSFFRIPTKVQQLVGEWVN
                     VPESCRWRLGGPGVGACLGQKTSLGRRAWVVQGKFRVVLGPLNHQQFTHFLPGAAGLW
                     RMATIIRSYTGDSLTWDVKLVLGEDAGRPWRLGSRMGLGITVWLGRRPDSIIVRAPAM
                     AET"
     gene            11881..14592
                     /gene="tssH"
                     /locus_tag="PK710_03955"
     CDS             11881..14592
                     /gene="tssH"
                     /locus_tag="PK710_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HOT08898.1"
                     /translation="MSEISRVALFGKLNATAYKAIESATVFCKIRGNPYVELVHWLHQ
                     ILQLQDSDVHRLIRAFDLNPSRLAKDMTESLDKLPRGSSSISDLSSHLDDAVERGWVY
                     ATLMFKESQVRTGHLIFGMLKTTSLRNVLFGISGEFAKIKVERLSEEFSKILDDSPEA
                     RMRATDGFQVGGGAMPGEASGAMAPAAMGKQEALARYAVDLTERARKGEMDPIVGRDD
                     EIRQIIDILMRRRQNNPILTGEAGVGKTAVVEGFAQRIAAGDVPPAMKDVSLLSLDIG
                     LLQAGASMKGEFENRLRQVIEEVQASPKPIILFIDEAHTLIGAGGAAGTGDAANLLKP
                     ALARGTLRTVAATTWAEYKKHIEKDPALTRRFQVVQVLEPTEDKAVLMMRGMASMLEK
                     HHRVQVLDEALEASVRLSHRYIPARQLPDKSVSLLDTACARVAISQHATPPEVEDSTR
                     RIQALECELDIIDREQAVGVEIGDRRQEAQAKLNVERENLAKLDERWKAEKKIVDEIL
                     ALRAQLREGFKAIDETAGTLESSVEPQPVADEQRGEKLEKLRQLQGELADLQKETPLI
                     LPSVDEQAVASVVGDWTGIPVGRMVHDEIGTVLNLAKAMGQRVLGQDHGLEMIARRIK
                     TSRAGLENPNKPIGVFLLAGPSGVGKTETALTLAEQLYGGEQNLITINMSEFQEAHTL
                     STLKGAPPGYVGYGEGGILTEAVRRRPYSVVLLDEVEKAHSDVHEIFFQVFDKGWMED
                     GEGRLIDFKNTLILLTSNVGSDLIMNMCADPELIPTSEAIANALRPDLLKVFPAALLG
                     RLVVIPYYPLTEQVIGGIARLQLDRIAKRVQQNHGIPFVYDDAAVSLIVSRCSELESG
                     GRMIDAILTNTMLPTVSQELLSRMMLGNPVQSITVSAADSEFTYTFG"
     gene            14636..16846
                     /gene="tssI"
                     /locus_tag="PK710_03960"
     CDS             14636..16846
                     /gene="tssI"
                     /locus_tag="PK710_03960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HOT08899.1"
                     /translation="MLLSTPIHVTTPLGDALQLRSMSGTEELGRLFCFDVELVSRDAT
                     IDLASLIGKDMTVSVETPAGERHFHGTVAQLQFMGEVGNFALYKATLRPWLWRLTMRS
                     GCRIFKDMSIAEIITEIFREHGLTDFKESLSGDYEQREYTVQYRESDFDFVSRWMERV
                     GIYYYFMHEEGRVSLVLADGYSAHETLSGYEEVPFFPPHEGRRERDHITTWTSSRQMT
                     CSACVFKGYDFTRPTAEVISKLVSRDELADAKREAFDYPGSLTSEMQGEGLSRVCVEA
                     FQAHQERVSGQGEVLGLSPGRLFRLTNHPREDQNQEYLIVSASYEARVVDYESVSNTE
                     DTFDFRCEFSAIPSKTPFRSQAITPWPKIFGPQTAKVVGDEGEEIWTDEYGRVKVQFH
                     WDREGRSRGSNSGWVRVAQAWAGAGWGAIHIPRVGQEVIVEHLEGDPDCPIVTGRVYN
                     ANNMPPYALPDNHSQSGFKSRTIGGAAENFNELRFDDKKDEEQVYLQAEKNLDTYVKN
                     DESHIVDHDRKKDIKNDETTTVGGNRTETVEKDETLAIKGNRTETVEKDETLTIKGSR
                     KETVEKDETLTVKGSRAETVEKDETLTIKGSRTETVEEDETLTIKGSRTREVGKDEKV
                     TIKGSRARKVSKDESIEISGARTLRVSMDEKVEVSSNGKRLVKGNDEITVKGKLVVSA
                     LQGLELKVGTGSIVIDAGGNITIKGLMVKVEGSAMVDVKASGVLSLAGAIIKGG"
BASE COUNT         3637 a         4044 c         5504 g         3851 t
ORIGIN      
        1 tgtggatgtg atggggctcg atgattggct ggagcgttgg aacccatcga ttttattgag
       61 aatatctgat gaacgggaga ttccgatctc gttttcgagt ctcgatgatt ttcacccgga
      121 cgcgctcttc gatcggctac cgatttttgc ggaattgcgt cttttgcagc aagaggcacg
      181 gcagggcaag ggcatcgttg ggcaagggga gtgtggttcg agggaatcca cgcaagacac
      241 gttggcgcgg ttattgggaa agcccaatgc gggtggggca tcgtcatcgc ccctgcaaga
      301 cttttttcat cggctcgtgg agcctcacgt cgtaacgtcg gaaacggatg ggatgaacgc
      361 tcagctcgaa gtgctgcatg aggcatccgg cgaactcatg catacgttgt tgcatcattc
      421 tgcgtttcaa cggctggaat cgatctggcg ggggctcgac tttttgcttc gtttcggaga
      481 gggattcgac gtttccgtaa gcaatctcag ccgggaggag cttgaagcat gggtgctcgt
      541 cgacgatcct ttggcaacgc ccttgggtcg aaggctgttg ggggaagcgg caggagagcc
      601 cgcgtggtcg ctcgtattgg gagcgtattc gtttggtcga aatgcccgtg atgtgatgtt
      661 gttgcgtcga ttgtcctcga tattggccga agcgggggtg cccatgattg cgtcgtatcg
      721 ggcgagcgaa ggtggtgagg ccgaggtgcc ttcgtcgtgg agggagttgc gatcgagtcg
      781 tgaggcgtcg atgattgggc tggcgctgcc gcgatttttg ttgcgattgc cgtatgggaa
      841 acgaacggat gagatcagcc ggtttccgtg ggaagagcaa accacgccac cgcagcatga
      901 ccgttatctt tgggggcatc ctgggtggtt ggtggcgtgt ttgttgggtc gaagggtgat
      961 cgattcgggt acgaagccat cgttatcgat gagcggatta ccggtgcatt ccttcctggg
     1021 ggaaggacag atggagcaaa cgccctgtgc ggagtggtgg ttgacggagc aggaggctgg
     1081 tcgatggctc gacgatggga tgataccttt gtgttctgta cgggggcggg atgaagtgcg
     1141 gttgattcgg gttcaatccc tggcggcccc gtcggctggg ttggctggat tttgagggat
     1201 tcattgcgtc acgtcggata atccaacggc aactgtcgtt tctcctcgct gtggtcgggt
     1261 ccggagtata gatttctttt ttcgacatgc agccttgtga atctggcgtt gagtatgccg
     1321 aaatcgatac gtttttcggt cagcgtgggg ctgggtatgc gggagccacc atacgaggca
     1381 ttgatttatg ccgtagcgat ctatcgggat tggactttgg gggagcgaaa ttcgtgggcg
     1441 tggatttgac cgatgcgaac ttgtcccggt gcaatctgcg gggagcgatt ttttcgaaca
     1501 gcgagtgtgg ggggatagat gtatcggggg cgaatttaca gggggtgcgg tttgaagagt
     1561 cacggttatc gacacgaaaa attgggggag cggatttgca gggggcggcg ttcgtgcaag
     1621 cggacttgac cgaagcggat tttcggcaag tcaaggtggt gggggcgcgt ttcgagcgat
     1681 gtttgttgac caggattcag gctgagggtg tggattttcg cgggtgtatt ctcaacgaca
     1741 gcacgttgtc gaaagctcgt ttgaagggag cgcgtttcga tggggtttgc gcatcgcagg
     1801 cctgcttcga aaatgcggat ttgacaaagg cggttctgat tcgtgtctcg gcgccctttg
     1861 cccgttttgc aggtgccttg ttggaagagg cgaatgcgga tgatgcggac ttgacccatg
     1921 cgagtttttt gcgggtgaaa gcgcagcggg cattgtttcg acgcgccaag ttgacacgaa
     1981 cgacatggca agctgcggac gtacgtggtg ttcgtttcga tatgtctgag ttgaattacg
     2041 cggatttttc ccacgccctt gcggacctgg catcgtttca gaaagcggtg ttggaccacg
     2101 ctaacttgca tcgtacgagc cttgcgtcat cgatcttgtc tggggcgagc atgaatggtg
     2161 tacgcaaaac cgatcccgat cgagccaggg cggaagactt tacaacgggc aggaggtagt
     2221 catgacgagg caagcagcgc tgcggttgta tccggaccct aaggaaagcg agagggtgca
     2281 tactccgcgg ggggcgagcg cgacggcaga aggcgagagg cttgtggtgc gcgacgctcg
     2341 tggggctatc gtggttgtat atgatgcgga ggcggggtct gcgacgatcg tggcgccggt
     2401 gggggattta cgtcttgctg cgccaacggg aagcgtagtg atcgaggcgg gtgaagacgt
     2461 ggagttatcc tcgcggcgga cggttcgaac acgcgcggtg gcggtggaaa gtgacgcgga
     2521 cgtaactcgg tttcgttcga aggcattcga agtggtgacg ggtgtatggc agacgacagc
     2581 ccggacggtg gtgcatgggg ttggcagttg gagccttggg gctgagcgtg tgttggagcg
     2641 agccaacgat gtggttcggg cggtgcaggg gttgatggag acacgggcgg gaagagtacg
     2701 gaccgtcgtt caggatacga cacaagttcg aagtggttcg acgagcatat cttcgaaaga
     2761 agatacgttc attgatggtc gtcgtgtgtt gttaggatga ggtgggagtc atggcgggtg
     2821 tagtatttcc ggcggcgacg atgaaagggg ggcaagcgat gtgtcttggt cccatcgatg
     2881 tatgcaaggt accggctcct ccggcgcctc cgattccgat gccatttccg aatattgcta
     2941 tgctcaacat ggcgctgaac acgacgacga cggtgttgtt cgagaacatg ccggtgttgg
     3001 tgcagatgtc ggaaacgccg ttgagtagtg gtgatgagcc gggggttgcg gggggagtga
     3061 tttccggcgt attcataggt ccgatgatgt gcaaggtggg cagtgggaag gtaaaagcac
     3121 agggaaaagg tgtgatggtg atgacgggga tgacctcgca taatgggaag aacccgaatg
     3181 tacctgtggg gatgatcgtg caggcggcga acaccaaggt gttggtatct ccttgatcca
     3241 ccccctcatt tttccccgag ccttttcttc acggtcacgg cagacgcgaa acggtcacgg
     3301 ccatcccatg ttgatcaccg cagtgcgcca gtcccatgtt tcgtagctct gggcaactgt
     3361 tggcggcggc ccaagggggg tcaaggaccg agccaaaacc aagaaccaaa attctcagct
     3421 cgtatccctt tcccattcgg gaataatgag gggatctgtt cggggtagtg tgggaaagtc
     3481 gagaccgcga tggatggaat gaaatgcagg aacggtgggg ccatggcgca aaaaaggtca
     3541 atcgcaccca aataggtgtg tattcctatg tatatgaaga atggcgaaat gatggtgggt
     3601 gaggcgatgt ggggcagtgg gggcgggttg gaggcgtgcc tgaatctgct tgaaatgggt
     3661 gcgccaaggg gtaggccctc gacgaccgac ggttgagaag aaagaaaaga gaagaaagaa
     3721 aagaacggag gagacgattc cgttgcgccc gccaagttgg gagagtacgt tcacgtgtcg
     3781 tctccaacgg gtttcccggt cgaggcgggt attgggctag cgttactcaa gccgaattag
     3841 accaatgatc gacgttacca aggtattaga gccggtgacc gacggggcac cttgtgggga
     3901 ggatttggag tatgatgaag cctttgtgga gcttcagctt gtagcgcaat acaagccgga
     3961 gcagcgtatg ggtgagtcgc tgatcccggc ggaggagcct gtatggcggg atgtggaatc
     4021 caaggcttcc gtattattcg agcgcacgaa ggatcttcgg gttgcggtgc atttattgaa
     4081 ggcgttgttg cacacgggag ggtttgtcga ggcagcgaaa ggcatgcagg tgttgcgcgc
     4141 gttggtgatc gagcattggc agtgcgtgca tccgcagctt gatccggacg atgatgacga
     4201 tccgacgatg cgattcaaca ttcttgcgga tttggcggat gcgtcgacga tgctgacctg
     4261 ggttcggaag attccgctgg ttacggttcg gggcattggc agttttgggt tgcgggacat
     4321 tgctttggcc tcggggcagg ttgtggctgt ggagggtgaa gtggcgcctt cgatggcgtt
     4381 gattgacggg gctttccacg aggcttcgca ggaggagctg gaaccggtgg gagcagcggc
     4441 ggcgagttgt gtcgaagcgc ttcgaagtat cgagaaggaa ttgatggcgc gcgcgcaggg
     4501 ggcgtcgccg ccaaatcttg cgccgcttcg ggagcttttt gagcaggcgg cgtatgcggt
     4561 gcaatctcgt ttggcggatc gggggcatgg gatggccggg gtggacctcg gctcggcggg
     4621 ggaagggcaa gtcatggcgg ctgggggtcc ggcgatcaag cgtttgtctg gggagatcac
     4681 ctcgcgggag gatgtgatca aggccatcga caagatctgc gagtattacg ccaaaaacga
     4741 gcctgccagc ccgctgcctt tgttgttggg gcgttgtcga cggttagtga acaaggattt
     4801 catgggcatc attcgcgaca tggcgccgga cggactatcg caggtcaagg ttctccgggg
     4861 tccagtggaa gaggaggacg acgattcatg agtgggcaaa aatttgttgg gcgtaaccgg
     4921 gcgccacgtg tccagatcga atacgatgtg gagttgtacg gggcagagaa gaaagtccag
     4981 cttccttttg tgatgggggt gatggcggac ttgtccggca atccggccga gccattgccg
     5041 aaggtggcgg accgaaagtt cctcgaggtg tccgtggaca acttcgatga tcggatgaag
     5101 gcgatgaaac cgcgggtggc gtttcaggtg cccaacaccc tgacggggga gggaaacttg
     5161 aacatcgatt tgacctttga aagcatggat gacttcacgc ctgcggcgat tgcccgaaag
     5221 gtcgaggcgc tgagcaagct gttgcaagca cggcaacagc tttcgaatct cgtgacatac
     5281 atggatggaa aaaccggtgc tgaagagctg attgcgagag tcatggcaga ccccacgttg
     5341 cttcaagcgt tggcgtcggc accgaaagat ggcgaagggc agggaggctg accatggcag
     5401 acagtgagac cgtcaaatcg gaagggcagc tacagactct cgaggtatct gatttcgagt
     5461 cccttttgca aaaagagttt cggcctcaga cggacaatgc gcgtcaggaa gtgcaaaacg
     5521 cggtgcgcac cttggctgag caggcgcttc aaggttcggt gaccgtttcg gacgatgtgg
     5581 tccagaccat caagcagatc attgcggaga tcgacaagaa gcttgctgaa cagatcaacg
     5641 tgattttgca tcatgccgac tttcagaagc tcgagggtgc gtggcgtggt ctgcactacc
     5701 tcgtgaacaa cacagagacc gacgagatgc tcaagatccg tgtcctgaac gtctcgaaaa
     5761 aagatctgca caagaccttg aagaagttca aaggggcggc gtgggatcaa agtccgattt
     5821 tcaaagccat atacgaagag gaatacggca agtttggggg tgagccttat gggtgtttgg
     5881 tgggggatta ttacttcgac cacagcgctc cggatgtgga gttgttggga gagatggcga
     5941 agatttccgc cgccgcgcat gcgccattca tcacgggaac cgcatcatcg ttgttccaga
     6001 tgcaatcctg gagcgaactt gccaacccac gcgacctttc caaaatcttc gaaaccccag
     6061 aatacgcagc ctggaaatcc ctccgggagt ccgaggattc caaatacctc ggcttggcgc
     6121 tgccaaggtt tttggctcgc cgtccttacg gcgccaaaac caatcctgtc gagggcttcg
     6181 atttcgaaga acagatctcg ggttccgcgg aagaccaatt cacatggtcc aactccgcct
     6241 atgccatggc ggtcaacatc aaccgttcgt tcaaactcta cggttggtgc tctcgcatcc
     6301 gtggcatcga gtccgggggt gctgtcggtg gattggccac gtacacgttt cccaccgacg
     6361 acggtggggt ggagatggcg tgtcccaccg aaatcggaat cagtgatcgt cgcgaggccg
     6421 agctggccaa aaacggtttc atgccgttgg tacacaagaa aaacactgat ttcgccgcct
     6481 tcatcggggc tcaatccctg cacaaacctg ctgtgtacga cgatccggat gcatccgcca
     6541 atgcggctct tgcggcgcgt cttccctatc tattcgcaac gtgtcgtttc gcccactatc
     6601 tcaagtgcat cgttcgcgac aaagtgggct ctttcaagga acgcgacgac atgaagcgat
     6661 ggcttcagga ttgggtcata cagtatgtcg acggtgatcc agcgcattct tccgaagagg
     6721 tgaaagcgca gaagccgttg gccgcggcag aggtggaggt gcaagaactc gaaggaaatc
     6781 cggggtttta ctccgcacag tttttcttgc gacctcatta tcaacttgag ggactcaccg
     6841 tttcgttgcg gttggtgtcc aagttgccgt ctgccaaagg cggttgattg acaacgtgaa
     6901 gcatctggcg gttcgcaaca gtcgctgagg cgtttggctg gcctttgcgg ctatgacgaa
     6961 tgaaatggga ctacgtgaat agaaaggaac agaaccatgg cagttgatat gttcatcaag
     7021 attggggaca tcaagggcga gtccatggac gacaagcaca aggacgaaat cgatgtcctt
     7081 gcctggagct ggggtggctc gcaatcgggt tcgttgcata cgggttcggg aggcggagcc
     7141 ggcaaggtca gcatccagga cctgacgttg accaaatggc tcgacaaatc atctcccgtc
     7201 ctttttcaaa ccatgtgtga aggaaaacac atcaaagaaa ccgtgttgac ggttcgtaag
     7261 gcgggcggaa cgccggtcga atacctcaag atcacgctca tcgatgcgat cgtgacttcg
     7321 gtctcgttgg gaggttccgg cggcgaggag cgcttgacgg agaacgtcag cctgaacttc
     7381 gccaaattca agctcgacta cgtgccccaa aaagaagatg gttcggccat gccagcggtg
     7441 cagatggcgt ggaatatcgc tgccaacacg ccgaaggtat agtgggtcga agggttctgg
     7501 gggcaagctc cggaatggtc accgccacct gccattagct gctgtggaca cgtttattag
     7561 gtggttgtga tcgaattcct taatgcttgc cacccggcag ccctgttgcc gatgcacaaa
     7621 atggagggac gcgatgctcg ctgaggacaa gctccgcgca ggtcaagtcg acgaagccct
     7681 tgccttgctt caagacgagg tacggaaaag ccccggtaac gttaggtatc gcgtctttct
     7741 atttcaactt ctatccgttc tcggacagtg ggatcgggcc tggttgcagc ttaagacatc
     7801 gggagacctc gacccgcaat gcctacccat ggttcaaacc tatcgcgagt tgcttcgttg
     7861 tgaggtgttt cgctcgcacg tgttcgcggg gtcgaaaaca ccggttttcc tgggtgaacc
     7921 gcagccttgg gcggcgctgc tcgtacaggc gcttcgacgt accgccgaag gcaagcatga
     7981 cgacgcacag gagcttcgtt ctcaggcgtt tgactccgcg cccaccactt cgggacaagt
     8041 cgatggcaaa cgtttttcgt ggatcgccga tggggatgta cgtcttggtc ccgtccttga
     8101 gatgatagtt catgggcgtt acacctggct tcccttccac caagtgcgtt cgttgcgcat
     8161 cgagcctccc aaagacttac gggacctggt ctggatgccg gtcattctca cgctcgtgac
     8221 tggaggcgag ttggttgctt tcattccctc ccgttatcca ggggctgaat cccattccga
     8281 cgcggccgtt cgattggcca ggcgtactga ttggaccgaa atccctggtg gatatcttgg
     8341 cgcgggacag cgcgtgtgga cgacggacga gggcgagcat gcgttgttcg atgtgcgtga
     8401 agtggatctc gacgtggatg ttgtcgacga gaatgtagtc accgatggct gaactcacct
     8461 acagagaaag gcttcaacct tcgctgctcg atcgtcttac cgacgatcaa cgagatcagt
     8521 cgcaggaatc gcggttggat cgggtgatca gtccgagtcg gctacgggag tgtgtgcgaa
     8581 gggatctggt ctggttattc aacaccacca acttgtcgag tttgaaatct gagatcaacg
     8641 agtatccctt ggtggcgcaa tccacgttga attatggcct tccagacttg gccgggcaca
     8701 cggtgatgag catcgacgtg gtgtcgatcg aacggctgct tcgaaacgcc atctggagtt
     8761 ttgagccgag attgattcgc aactcggtgc aggtgaggct cgatgtagcg ttggacgaga
     8821 tgacgcacaa tgcgatgaca tttacgatcg aggcggagtt gtgggcgcag cctttgccgt
     8881 tgcgtttgta cttcaagacg ggcattgatt tggagacggg ggaagcgacg gtggaagagg
     8941 tgggaaaggg gcagaggggc tgagatggat ccgcgcttcc ttggctatta caatcgggag
     9001 ttgcagttca ttcgagagat ggggggggag tttgcgcgcg agtatccaaa aatagccgga
     9061 aggcttggca tggacggatt cgatgtggcg gatccgtacg tggaacgctt gctggaaggt
     9121 tttgcttttt tgaccgcgag gttgcagctc aaaatcgatg atgaatttcc gcgcttcacg
     9181 cagcatttgc tcgaaatggt gtaccctcac tatttggccc cgacgccttc gatggcggtg
     9241 gtgcagtttc acccgagcct caaggaaggc gctctcgaac ggggcgtaac catcgctcgt
     9301 ggtacaccga tgaggagcct catcggcaaa gacgagcaaa cggcatgtga gtatcgaacg
     9361 gcgcatgacg ttgtgttgtg gccgatcgag atcgtgggct tcgagtatcg cgccaacttg
     9421 ggagatgtcc taggtcttgg agagcgcata aggcatgcgc aagcgggtgc gcggcttcgt
     9481 ctacgaacga ccttaggcat gacctttgac aagcttgctt tggaagagct tgtgttgttt
     9541 ttgcggggag agtgggaagt ctcgacgcgt ttgtatgaag ccctggtggg tcataccgtg
     9601 gcggtttttg caagacccgt gggagaagga cgatgggtgg ctctgggtcg cggtgcggtg
     9661 caagcgcttg gattcgacga cgatcatgcg ctgttgccgt tcggtcccca atcctttcag
     9721 gggtatcgtc tgctgcagga gtattacgct ttcccgcaac ggtttttgtt cgttcagctt
     9781 gcgggtcttg ggcctgtcgt tcgcgcgtgt acaacgcaag agcttgaaat tgttatacta
     9841 ttcgatagta aggactcgct gctggacaat gcgatcgagc cgaatttgct ggctcttcat
     9901 tgcgcgccgg cgatcaatct tttcccacga cgggcggatc gaatccatct ctccaaccag
     9961 gcgcgggagt atcatgtcgt cccggaccag acacgtccca tggacttcga agtgtattct
    10021 gtcatggagg tcgtgggcca cggtcaggcc gttcagcagc gcttctttcc gttttatgaa
    10081 tgcaacgaag ccaccgccgg cgagcaggac acgtttttca cgcttcatcg tatgccgcga
    10141 aggctctccg acaaacaacg tcgcaccggt ccgcgttcta cctatgtagg cagtgaagtg
    10201 ttcatcagtc tggtggacgg ggagcagggg cctttccgtt cttccctcaa gcagcttgcg
    10261 atcgagacgt tgtgtacgaa tcgggatctt gccttgcaca tgccccttgg gcagggaagc
    10321 acggattttt cgctgcaaac aggcgcgcct gtgaaagcga ttcgttgtgt ggcgggcccg
    10381 acacagcctc ggccttcgca tgcccatggt gagatggcct ggcgtttgat cagtcacttg
    10441 tcgttgaact atttatcgtt gaccgacggt cctgcgggca agggagccgc cgccttgcgg
    10501 gaattgctcg tgctatatgc ggatcaagag caagccgcct tgcgacgcga gatcgatagt
    10561 gttctttcgg tatcgtccac accggtgatt cgtcatcttc ccgtggatgg gatgatggcg
    10621 tacgggcgtg gcatcgagat cacgctcacg tgtgatgaaa ccggattttt gggtgcgggg
    10681 gtatttcttc ttggagccgt attggaaaga ttttttgctc gctatgtgtc catcaacgct
    10741 ttcaccgaga ccgtgttgcg aaccgtacag cgcggtgagg tcatgcgatg gcagccgcgg
    10801 atcggaacaa cccaggcgct ttagcgctgc tctccgaact cgagagccac cctgaatctt
    10861 tcgatctatt tctcgctttg cggagaatcg agtgtgtctt ccgacaaaac gctcgcctag
    10921 gtgaaggaat tcatccatcg gatgagccgg ttcgtctggc ccaagagccc tctctggcgt
    10981 tcacgtccaa cactctcagt ggggtgcgtg tccggggttc gattccgcgt gtgcgaaccc
    11041 ggatccttgg gttgttgggt cctttcggcc ctcttccttt gcatttgacc gagtacgcct
    11101 atgaccggag tcgcaatcga aacgacccta cgtttgctca cttcctcgac ctatttcatc
    11161 atcggatcct gtctttgtat tatcgagcct gggccaacac cgaacccacc gtggcgttcg
    11221 atcgtcctca gacggcgcgt tttactcgat acattgcctc tctcatcggt atgggtatgc
    11281 cgtcgttttt gtgtcgcgat gagcttcccg atcatgtgcg cttttttttc gcgggtcgtt
    11341 atgcggctca cagtcgcaac gcggatgggt tggcggcgct gcttcattct ttctttcgta
    11401 tacccacgaa ggtgcagcaa ctcgtgggcg aatgggtgaa tgtcccggag tcttgtcgct
    11461 ggcgtttggg aggccccggt gtgggcgctt gtcttggaca aaaaacgtcc ttgggacgaa
    11521 gggcatgggt ggtgcaagga aagtttcgcg ttgttctggg tcctctcaat caccaacaat
    11581 ttacgcattt tcttcccggt gccgcggggc tttggcgaat ggcaaccatc attcgcagtt
    11641 atacgggaga tagtctgacg tgggatgtga aactggtgct aggcgaagat gctggtcgtc
    11701 cgtggcgttt ggggtcacgc atgggattgg gaatcaccgt ttggttgggc cgacgtcccg
    11761 acagcatcat cgttcgcgcc cccgcgatgg cggaaacgta atcctatctt tgaaacagaa
    11821 tgtgtggaac cgattcatgt cgtgaacagg gctgactgcc cagcaaagaa agagtcgacc
    11881 atgtccgaga tcagtcgtgt tgctctattc ggaaagctca atgccaccgc ctacaaggcc
    11941 atcgagagcg ccaccgtatt ttgcaaaatc cgagggaatc cttacgtgga actcgttcat
    12001 tggctccacc agatcctgca attgcaagac tcggatgtgc atcgattgat tcgtgctttc
    12061 gacctcaacc ccagtcgtct tgccaaggac atgaccgagt ccctggacaa actccctcga
    12121 ggatcgtcgt cgatttctga tctgtcttcc catctcgacg acgcggtgga gcgtggttgg
    12181 gtttatgcca cgttgatgtt caaagaatcg caagtgcgca cgggacacct gatattcgga
    12241 atgttgaaga ccacgtccct tcgcaatgtg ttgttcggaa tatcgggcga gtttgccaaa
    12301 atcaaagtcg agcgtttgtc ggaagaattc tccaaaatcc tcgatgattc tccggaagcg
    12361 cggatgcgcg ccacggatgg atttcaagtg ggaggcggag cgatgccggg agaggccagt
    12421 ggagccatgg cgccggcggc gatgggaaaa caagaagctc ttgctcgata tgccgtggat
    12481 ctcaccgagc gcgctcgaaa aggggagatg gaccccatcg tgggtcgcga cgatgagatt
    12541 cggcagatca tcgatattct gatgcgtcga cggcagaaca atcccatttt gacgggggag
    12601 gcgggcgtag gaaaaacggc ggtcgtcgaa ggatttgcgc aacggattgc ggccggcgat
    12661 gtacctcctg cgatgaaaga cgtctctctt ttgtcgttgg acattggctt gttgcaagcg
    12721 ggtgcgagca tgaaggggga gttcgaaaac cgtttgcgtc aggtgatcga ggaagtgcag
    12781 gcttccccca agccgatcat cctgttcatc gatgaagctc atacgctcat tggggcaggg
    12841 ggtgcggcag gtacgggtga tgcggccaac ctcctcaagc cagctttggc gcgcggcacg
    12901 ttgcgcacgg ttgcggcaac cacatgggcg gaatacaaaa aacacatcga aaaagacccg
    12961 gcgctaactc gtcgtttcca ggtggtgcaa gtgctcgagc ccacggaaga caaggccgta
    13021 ttgatgatgc ggggaatggc ttcgatgctc gagaaacatc acagggtaca ggtgctcgat
    13081 gaggcgctcg aagcctccgt gcgtctgtct caccgataca ttcccgcccg tcagctcccc
    13141 gacaagtccg tgagtttgtt ggatactgct tgcgctcggg tggccatcag ccagcatgcc
    13201 acgcctcccg aggtcgagga ttcgacgcga cgcatacagg cgctcgaatg cgagttggac
    13261 atcatcgatc gagaacaagc ggtaggagtc gagatcggag atcgtcgtca ggaagcgcaa
    13321 gccaaactga atgtggagcg cgaaaacctt gccaagctcg acgagcgttg gaaagccgag
    13381 aagaaaattg tcgatgagat tttggcgtta cgcgcgcagt tgcgtgaggg attcaaggcg
    13441 atcgatgaaa cggcaggcac gttggagtcc agcgtggagc cacagcctgt cgcggacgag
    13501 cagcggggcg agaaactcga aaaactgcgg caattgcaag gtgagttggc cgatttacaa
    13561 aaggaaacac ccctgatcct gccgagtgtc gatgaacagg ccgtggcttc ggtggtgggc
    13621 gattggacgg gaattccggt gggccgaatg gttcacgatg aaataggaac ggtgctcaac
    13681 ctagccaaag ccatgggaca gcgggtgttg gggcaggacc atggtttgga aatgattgcg
    13741 cgaaggatca aaacctctcg tgcgggcctc gagaatccga acaagccgat tggcgtgttt
    13801 ttgctggcgg ggccatcagg cgtgggcaaa acggagacag cgctcacgct tgccgagcag
    13861 ctttatggtg gcgagcaaaa cctcatcacc atcaacatga gcgagtttca agaagcgcat
    13921 acgctctcca cgctcaaagg cgcgccacca ggatatgtag gctatgggga aggaggaatc
    13981 ctgaccgagg ccgttcgtcg aagaccctac tccgtcgtgt tgctcgacga agtggagaag
    14041 gcgcactccg acgtgcatga gatcttcttt caggtcttcg acaaaggttg gatggaagat
    14101 ggcgaaggtc gtctgattga cttcaaaaat acgctcattc tgctcacgtc caatgtgggc
    14161 agtgacctca tcatgaatat gtgtgcggat ccggagttga ttcccacgag tgaagcgatt
    14221 gccaacgcgc ttcgtcctga tctgttgaaa gtgtttcctg ccgcgttgtt gggccgcctg
    14281 gtggtcatcc cctattatcc gctcaccgag caggtcatcg gcggcatcgc gcgactacaa
    14341 ctcgatcgga tcgccaagcg cgtacagcaa aatcatggca tccccttcgt gtacgacgac
    14401 gccgcggtca gcctcatcgt tagtcggtgc tccgaactcg aaagcggcgg tcgtatgatc
    14461 gatgccatcc ttaccaacac gatgcttccc acggtcagcc aggagttgct gtcgcggatg
    14521 atgctcggca atcccgtcca atccatcacg gtttctgcag ccgattcgga atttacctac
    14581 accttcggtt gagcgaacgc tcgacgtcga aacttgactc aggcgaggac gtcccatgtt
    14641 gctttccact cccattcacg tgacgacccc tttgggggat gcccttcagc ttcggagcat
    14701 gtcgggaaca gaggagctgg ggagattgtt ttgtttcgat gtcgagttgg tgagccgtga
    14761 tgccacaatc gatctggcga gcttgattgg aaaggacatg acggtttctg tcgaaacacc
    14821 ggctggagag cgtcactttc acggaaccgt cgcgcaattg caattcatgg gcgaggtggg
    14881 aaactttgct ctgtataagg ctacgcttcg cccttggttg tggcgtctga cgatgcgttc
    14941 cggatgccgg atcttcaaag acatgagcat tgcggagatc atcacggaga tctttcgcga
    15001 gcatggcctc acggacttca aagagtccct ctcgggtgac tacgagcagc gtgagtacac
    15061 ggtgcaatac cgggagtcag acttcgattt cgtgagccga tggatggaaa gagttggaat
    15121 ttattactat tttatgcatg aggaggggcg agtctccctg gtgttggcgg atggttatag
    15181 cgctcatgaa accttgtctg gctatgaaga ggtgccgttc tttcctcctc atgaaggtcg
    15241 tcgtgaacga gatcatataa ctacgtggac ctcgtccagg cagatgactt gctcggcttg
    15301 tgttttcaaa ggctatgact tcacgcgtcc gacggcggag gtgatttcga aactcgtgtc
    15361 gcgcgacgaa ctggcggacg ccaaaaggga ggcattcgat tatcccggaa gtctcacttc
    15421 cgaaatgcaa ggggaggggt tgtctcgtgt gtgcgtcgaa gcctttcaag cacatcagga
    15481 gcgagtatcg ggacaggggg aggttctggg tctatcgccg ggaaggctat ttcgtctcac
    15541 caatcatccg cgggaggatc aaaaccaaga atatctgatc gtttctgcat cgtacgaggc
    15601 gcgggtggtc gactacgagt ctgtgtcgaa taccgaggat accttcgatt ttcgatgcga
    15661 gttttccgcc attccgagca agactccgtt tcgatcgcag gccatcacac cgtggccaaa
    15721 gatcttcggt ccccagacgg caaaggtcgt cggtgatgag ggtgaggaga tatggacgga
    15781 tgagtatggt cgcgtgaaag tgcagttcca ctgggaccga gagggccgga gtcgtggcag
    15841 caacagtggt tgggttcgcg tggcgcaagc gtgggcggga gcaggttggg gcgctattca
    15901 cattccgagg gtcggccagg aagtcattgt cgaacatctc gaaggcgatc ctgattgtcc
    15961 catcgttacc ggtcgtgtgt acaacgccaa caacatgccg ccctacgcac taccggacaa
    16021 tcatagccag agcggtttca agagcaggac aattggtggc gcggcggaaa acttcaatga
    16081 actgcgcttc gatgacaaaa aagacgaaga gcaggtttat ctgcaagcgg agaaaaacct
    16141 cgatacctac gtcaaaaatg acgaatccca tatcgtggac cacgatcgaa aaaaggatat
    16201 caaaaacgac gagacaacga cggtgggggg caatcgcacg gagactgtag agaaagatga
    16261 aacgctagcg atcaagggca atcgcacgga gacggtagag aaagatgaaa cgctgacgat
    16321 caaggggagt cgcaaggaga cggtagagaa agatgagacg ctgacggtca aggggagtcg
    16381 cgcggagact gtggagaaag atgaaacgct gacgatcaag gggagtcgca cggagacggt
    16441 agaggaagat gagacgctga cgatcaaggg gagtcgaacg cgagaggtgg gtaaggacga
    16501 gaaggttacc attaagggaa gtcgtgcgcg gaaggtgtcg aaggacgagt cgattgagat
    16561 cagtggtgct cgcacactcc gtgtgagcat ggacgaaaag gtggaggtga gcagcaatgg
    16621 aaaacggtta gtgaagggta atgacgagat tacggtcaaa ggcaaactgg tggtgagtgc
    16681 gcttcaggga ctggagttga aagtgggaac ggggagcatc gtgatcgatg cgggcggtaa
    16741 catcacgatc aaaggactga tggtgaaggt ggagggcagc gcgatggtgg acgtgaaggc
    16801 gtcgggcgtg ttgagcctgg cgggagcgat catcaaaggg ggatgattcc ggcagtacgc
    16861 tttccatccg ttgcggcgaa acgcgtcgta tgggtttgga gcatttcgtg tgcgagcgca
    16921 tgagccgtcc attgttagat cgtggggcaa gtacctgctt cgatggatgg caacggcaat
    16981 tgccagttgg ctgtcgacgt ccatcttgac gttggggttg cgagaatcat gaaccc
//
LOCUS       DAOSOR010000043        16889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_3423_length_16889_cov_4.723001, whole genome shotgun sequence.
ACCESSION   DAOSOR010000043 DAOSOR010000000
VERSION     DAOSOR010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 16889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16889
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3423_length_16889_cov_4.723001"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="PK710_03965"
     CDS             <1..793
                     /locus_tag="PK710_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08900.1"
                     /translation="ATADACDGPDGRDNATGIMMTKLAALFGSFSSTEWSANAKKGIW
                     SMLLRVRDYNGEANDDRVRLEWFVPSQFSADKADNDVEPVWDGSDAWPIRSESLEAVD
                     GGEYSFEKPKYFDTHAYVTEGILVGSFPESVFQIDDDYALDFTGSFITARIESTAQGW
                     MLTEGLLASRWALSSILAQIGRLHLMNKPVCTDHIAYPELKREFCSYADIYSGVGTPT
                     TPCDSVSLGMRFEARPALLGTIIIKDPSERFCDPSVDPAFDHCGN"
     gene            complement(836..1819)
                     /locus_tag="PK710_03970"
                     /pseudo
     CDS             complement(836..1819)
                     /locus_tag="PK710_03970"
                     /inference="COORDINATES: protein motif:HMM:NF004042.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
     gene            complement(2072..3235)
                     /locus_tag="PK710_03975"
     CDS             complement(2072..3235)
                     /locus_tag="PK710_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08901.1"
                     /translation="MSACSYYDSSLLAAHHTSDAGDDEGLEPGQDVVQEVVQDVVQEE
                     TTIPQEAAIDTGEDIHPSDVGQDAICVSAQPPDPPSHGDGGEIDFVVAVHQVELGDTQ
                     GDAKTIGFDLDARCTCQGDGDSCFREAWATADSCDGPGGRDNAVGTLLPALDPKLTGF
                     GSAVWSEEAVRGDWGLLLHVREYNGKEDDSQVRLDWLVPFHFSADKEGGTHPAWDGND
                     AWPIRDSCLIDSDVLKPRMRDLHAYVTKGTLVAILPESTLVVTNGFSLDLSGTLLTAK
                     IVESPQGWQLQDGVLAARWKADAFLSQLDRMIVLGLNVCKDHPAYSTMKKRICAHVDI
                     YSGVSSPTTPCNSVSMGMRFEAMPAKMGPVLPSSEQASACAPGKESAGDSCSQ"
     gene            complement(3677..9028)
                     /locus_tag="PK710_03980"
     CDS             complement(3677..9028)
                     /locus_tag="PK710_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08902.1"
                     /translation="MSTDSDNKNGQMAGDWDSALEAWDESPLNPSLPDDKTPPPPGTE
                     ASESAGDAPATLKPPAGHLVSQEEDDDDECTVVGQVPAELLADSVRGLGGASGLSSLF
                     SRGSQPTIPAEDEVAQQPASAPTSSSEPFSLRTLASRTDEANDADLSHSFDPFADLRD
                     DLISPAPADATPVPAAVDTPLPGLQPNELDEPVSAGPKLLEPEDRKYPQDEVTEVFRG
                     QGITVTSDSPATSRSEQLASADSAQESALVRSVSPPPLEPPKNPWPDERDASAHLMER
                     EMRTEWEARAAWFAEEAAARDIGEQRARIMLAISELCAMVGDDEKAVAVAKAASDMDK
                     DHPLLQRQTRYLANRERRWADVKNELETEARTAPTPEAKVHALLMDAHLNAQLHLDDD
                     VSAKQIDLAARIVPSDPRPHLTKLIHYLNDETQEKEPPRWPDVPPLASLEQGAALVAR
                     IREAEKAPAEPGETVQAYEALPRAKAALRKGDTKAAARALRALDDVRGMGGGALWLAA
                     ALASQHQDSRPQAMNWLDKLATGPHHAVALRMLAMRAVESADRNALDRALAEPGSNAF
                     SPADRVVLAALFQEKYGPVEPLVSTLLGQQNLAALGAAARSALTDVRDASRIDACSMG
                     ALPLRCLSAVGRAAALRLDSDEFWQRVAALRDSSFNGAVARLLELEMAIEKGFTDSII
                     DHIASWGGDHIRERDNALVAAIIAESLGDSERASQEYARVLSMEPSFEAAVRALRSME
                     PDLHANRVVELAARAEGAKGAAYALEAALLRGPGEDDYIDLLRLSHDKDPSLPYAASL
                     AEQYARHRGDADGVIEWLRVRRETTNDPIEASLDACREALLMVERDTSLAVALMERAC
                     RARPKDAALRALYERFSEERPEDWIAWRLDRANESEGMEKAILLLEAALESERQAHVD
                     DAAKLASQALDCGAGELALLCIERCELAGASTTHLTDLLMSQVRQEELPMEKRREVLE
                     QLAELDEVGRGDMASALLWHRTILEQPPQHLLSLRRIEHAFIGMGRDGDLEPIASELV
                     RALQGPEVDAHAVVASRIRLRESSWTDLADLARAAAQQPNPSLWALRSSFAHAREQGD
                     DAIVVQTAKTLSGLSDNEIESAALLVHAAQALTRMDEQEEASQLFQMAIDREPGFFQA
                     HLELVDVLEKTGEYARAAEELESLARKSNVDMHRAELWYRAAKMWIDKVQERERGRRA
                     FEEASDIDIGYRDVFDNLRVLYTEEKDAAQLALLLERRLEATTDPQERVEIEVLRGKV
                     LAEVGEIGGAKRALAAALEANPDHQPALEAFALTAAMEEDWGGAEQALLRLTRLVSNV
                     QQQGEIYRKLGAIYLDHLPNLERAESCLREVLKRNPEDIQAQERLIDVFRATGDASKA
                     IELGTQLVNQAQSPDAKREQTIRLALIHEQLEGNANKAQEILDRLYKQAPSAASPLRA
                     LAEFHRRQGHEKSLELLLDRASKDTARALRTGRFSPDLFATLETVASIRDDEAGAAVA
                     RAVVDVLEGTREVQLSSFGPSACSVNLDDILAPEVLTPALRALLKRAGDLFETAFPMD
                     LKALRASAVPPSSTQMQEAIVAAASSMGVHNLQVFVSPALGPTCVPIQSNPPIVVLGS
                     AMLTTDEHAVRDFLLIRALKIVQARGSVLARTAPIDLVPTVAALVKTLAPSFQPVTID
                     ARRFAEATQKLSAAKPATVDPDMTALALEVGGALDNRASTLNIAINGWGDRAALLAQG
                     GLSVALKAIAWSGGHPSGPPASGKDRMNWIGRNAEARDLIVFVASDAYGEAIKRLGS"
     gene            complement(9025..13794)
                     /locus_tag="PK710_03985"
     CDS             complement(9025..13794)
                     /locus_tag="PK710_03985"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT08903.1"
                     /translation="MTSSDANSSGSTAPAASPSVEVPEVIQRHIDWLVREQEVATTDE
                     QRALLFFESAVARERIRDEAGAARDYLASYNANPAFREPIESLASLLERRKSFKNLGK
                     LFDALVRAATTPPQIARATLLQGSCLLEHESDPVRAKDAFEQAVTDDPDLAAAWLELE
                     IVSGKTEDKDLRIRAMEERVRLAEPTAWKALLLLDLAKEEHERGNGDKALDHIHAVEE
                     LDSPHRFRAALLLERLARREQREDLIAEALKKQASIILAAMQDESVGVDAVPSHVRTS
                     AHVASLFLRAAQAAQRSGDIDAATSLLDLAVSQLPEDGVVLLARMQAAEKAGDAQTAA
                     SLAQRLLPLRSGPLAAALWLRIFEGAAARGEREAALEALTNALSSDQGCIPARALQTD
                     LLLDSDPTAFAASLEAMAEHGLSDEAKGRSYLLAAWAWAILANDVSGAKAAMSQAAMF
                     GVEHGVVSRLARAFSSILEDDSWYEDSTRRLIAAGSGAEEHASLWFELARLRLQRKDP
                     ETEKALDSLSNVQAGAWLGRMLAAYALGLSKLDAEDSKASRSPDRIEALADVEEDPAI
                     ARALRVVAQLLRARAGDSQRAMKTLSVLHEQDNSDLLVSVLLADLLRMSDDAQAAARV
                     VSTSATSLEDAELAAALHLEAAFLFWKAKDKNAALQSIRAARDLLPEAAAAALLWATV
                     ALDTETIDGRRRMLDLNEECGADKVSLALERFAVESAEGGDDDQASAALDALERDALG
                     EIGVAGWLGRLVHAPEFEDATARERAMGGLETLGLRASAVVAAERYRFARSVEKDRDA
                     ARDLAQAWAMADGGVVPAIEWLVASCAAEDEASESSARRLLARHVEKETASALEASAS
                     LIDLTRSTPSDSPPLLDSSEPPAQLMNLELAPAGCDPRKRAKALLGLNDALDNRAKID
                     ARMLAGWSLLAAGEGEQALDVFRRVCAARDDDIVAWEGVRAAASMVDDVRWTATACER
                     LGALCSNDARAAEFFETAGLLWIDRGDDQERGELALAKGFQRDGHRFVCFDRLFRRVR
                     AREDNDYLLTLIARRLEFAEDPEEIAKMFWEQARVLRQKGDFAAAMAALENVTLMEPD
                     HVGALALSGEVYIRQGAFGEAVDALARLAAHKEAPAQQRLVSGMAAVDLCENKLGDHK
                     RSFEILMTLHTTGLSTLPVRERLAKAAATNEAWGQATAVLETLMHERENAPGRIQAAR
                     LAMVIHRDKTGDPSGALPSVVQLLSESPADAEAMQFLMDHPHVGDGQLRASLLSKARL
                     TLVRRVSMAPEPSDVVLLARISKEQGDLQRRQACLGVMVAMGRGGDDALQELESLDGR
                     VARTPQVALDDAVIAAIADPRDVGALPALFQQLAPVISEALGPTLVGLSVTKKNRVDP
                     RDGLPLRNEIAHWAGALGLSDFDLYVGGREPYGVTGVPGEKPFLVVGNEISAPLAPHA
                     RQAVARELFAIRRGISVLRTRDAPTVASIVIAVCNLVGTPIAAPPYAMVAETQRLIGK
                     ALPRRLKSVLQEICQAVAASQIDPIVWVRFALSSMDRMAAIAAGDVSLVLAEVFGVPR
                     NELQAIVAEDQRARELVAFVLSDRYLELRNQLGMGVR"
     gene            complement(13946..14527)
                     /locus_tag="PK710_03990"
     CDS             complement(13946..14527)
                     /locus_tag="PK710_03990"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT08904.1"
                     /translation="MQKTTSVFLGALVLVVTGLVVWGGRPRASPAVQIPALSSSVPEQ
                     VEPMTSLDAGSVPDMVQEASFSGVRGAKPGYVMLDGSKPPDLPPEAPRSVRFGVVLVQ
                     YRGAEHAPLGARSKMEALKTAHHLLEVAKNDFAAAVAQGDAGSTAAAGWMPRRVLEPA
                     PEYVLFTTPPGEVAGPIDTPTGYWIVKNIGKNK"
     gene            14743..>16889
                     /locus_tag="PK710_03995"
     CDS             14743..>16889
                     /locus_tag="PK710_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08905.1"
                     /translation="MRCPKCGKTFVVNKESPSPAAAPPAPGKVPPPPPSLRSSAAKRT
                     MLGVGSPIILPRGADPNALPPPPDDFDIGLPVVSPPVGKSASKAAAPVHADLPSAAKP
                     LPPFPPAARPAVAQRPAPPAPAAAPSTANDDDLADFGDLPLPVISASPKEAALKDLPA
                     TRASKTAPFPHLPAKAPASPADFGDLPSPIGSPTDFGDLPSPVASPAGFGDLPSPVAS
                     PAGFGDLPSPVIDLSRPDAGLPVVSTDQNRPARQITGAALGFGGPDLPSPRTSVSNEA
                     GFGDGFGELDLPLAATVGLPTVASSQAELPSPSDLFGNLPSTADPNANFPTPLGLNQQ
                     LPSRQGTPSNASVDDLFGPTPPSPGGLFPDGMPPAPPPSQAPTGLGSFPPIGTGGLSS
                     PPDGAISGQAAGSLASQADAFGSLPPAAPAGQATIQRQAGGGVAFGEVNLTDDGQGAS
                     ATTGSLAPGAGTSSYGERSLGGGDEDMEFGAIPQEDGEDKSIDAAPTALPTKAEVGVV
                     ALAPRKSKALKIILAAILVGLTGGIAMSATPLGAFGTHFLSDMLNSGRNTAMLDETVQ
                     KSRTALSIDTADQAQACIEQTDEAVNRAPRMASLSAYAAFIGFQWELRFGSDSAVHAH
                     ATALLARAIEIDKEAQHLELARAAQASSEGEIAKARQAIQELVRLDSNNIDAAVLEGE
                     LELRAKEFNNALVAWQRAAGLEKSARTSFGQARA"
BASE COUNT         3480 a         5128 c         4733 g         3548 t
ORIGIN      
        1 ggcgacagca gacgcctgcg atggccccga cggacgagac aatgcgacgg gcatcatgat
       61 gacgaagttg gctgctttgt ttggcagttt cagcagcacc gagtggagtg cgaatgcgaa
      121 gaagggcatt tggagtatgt tgttgcgggt ccgtgattac aacggcgaag cgaacgacga
      181 tcgagtgagg ctcgagtggt tcgtaccctc tcagttttcg gcagacaagg cggacaacga
      241 tgtggagccg gtatgggacg ggagcgatgc ctggccgatt cggtcagaat ccctggaggc
      301 ggtcgacggg ggtgagtata gctttgagaa gcccaaatat ttcgacacgc atgcgtacgt
      361 gacggagggg attctcgtgg ggagttttcc ggagagtgtg tttcagatcg atgatgatta
      421 tgcgttggat ttcacgggtt ctttcatcac ggcgcgcatc gagtccacgg cacaagggtg
      481 gatgttgaca gagggtttgc tggcctcacg ctgggcgctg agcagcattc tggcccagat
      541 cggtcgtctt cacctcatga acaagccagt atgtacggat cacatcgcct atccggagct
      601 gaaaagggag ttttgcagtt acgcggacat ctattcgggg gtgggaaccc ccacgactcc
      661 ctgcgattcc gtgtccttgg gaatgcggtt cgaagctcgt ccagcgttgc ttggaacgat
      721 catcatcaaa gatccttccg aaagattctg tgatccctcg gtcgatcctg cctttgatca
      781 ttgcgggaat tgatcggtaa cgtctggttt ctatccttgg ttacggagag cggcttcaga
      841 aagggtcact tctttcgaag tggcaggagt agcctcctgg atttcgcagc agatagtcct
      901 gatgttcgct ttctgcgtcg aaaaacggca tggcttccac aatctgcgta accaccgtgc
      961 catcgaattt cccagaggcg ttcgcttttt gcacaacatc gtgcgcaatc tgtttttgtt
     1021 cccccgattg gtagaaaaca accgatcgat actgcgatcc gatatcgttg ccttgccgat
     1081 tgatcgtggt tgggtcgtga atacgaaaga agtacttgag cagcgcttcg aaggagagtc
     1141 gagtggggtc gaagaccact ttgacggatt cggcatgacc ggttccccct cgtgaaacgg
     1201 ctttataatg agcggtatcc gcttggcccc cggtgtagcc gacttgggta tcgagcacac
     1261 ccggtagttt tcgcagcagg tcctcgagtc cccagaagca gcctccggca aggatggcga
     1321 cctcgaggtg ttcatcgact ccttttcggt tccaagccac ggcttcgaga gcggacgagg
     1381 acaagctggt ttctggggga tggcgttcgg catccgcgaa aagcgaggcg tattcgccat
     1441 agccttcgcg ttcgaggcta tgtgcgggaa tgaagcgtaa ggccgcggaa ttgatgcagt
     1501 atcgaagccc acggggcgca ggaccatcat cgaatacatg ccccaaatgg gaggaggcgc
     1561 gacgcgaacg cgcttcgacc cgaatcattc cgagcgagtg gtcttggtgt cgcgtcacgt
     1621 tcgattccac caaaggcttg gaaaagctcg gccaacccga tccggaatcg aacttgtcca
     1681 acgaactgaa cagtggctca ccagatacga tgtccacata gatccccgga tctttgaggt
     1741 tccaataggc gttgcggaat ggcggctcgg tacccgactg ctgcgtgacc ctgtatccct
     1801 cttcggatag tgtttttttt ctctgttcga gttgtgcatc cgtgaggttt cgccaatccc
     1861 acgacgcttt ctgctcttgc ggaggggtgg aaggaatggt cgcgcccttg tcatcgggct
     1921 tgcacccgat gctacccaac acggccatga ctccccccac gcttctgaac caacgattca
     1981 cgacgcttct ccatgaacaa gcaaaccatg attcgttaga tgccgaagtg ggcagaagcg
     2041 tcacgcgatt cgatgccagg cgacaggctg ttcattggct gcacgaatcg ccggcggatt
     2101 ccttgccagg cgcacaggcg gaagcttgtt cggatgaggg caggacgggt cccatttttg
     2161 ccggcatcgc ttcgaatctc atgcccatgg agaccgaatt gcatggtgtc gttggtgaac
     2221 tcaccccgga atagatgtcc acatgcgcac aaatccgctt tttcatggtc gaatacgcgg
     2281 gatgatcctt gcaaacgttc aaccccagaa cgatcatccg gtctagttgg ctcagaaacg
     2341 cgtccgcctt ccatcgcgcg gccagcaccc catcctgcag ttgccacccc tgcggcgact
     2401 ccacgatctt ggccgtcaac agtgtccccg acaaatccag cgaaaagccg ttcgtaacca
     2461 ccaacgtgct ctccggcaaa atagccacca gcgttccttt cgtcacgtaa gcgtgaaggt
     2521 cgcgcatacg aggtttgaga acatccgaat caatcaaaca cgaatcccga attggccatg
     2581 catcattgcc atcccaagcc ggatgagtgc ctccttcctt gtccgcggaa aaatggaaag
     2641 gcaccaacca atcgagccgc acctgcgaat cgtcttcttt cccattgtat tcccggacat
     2701 gaagcaaaag accccaatcc cctcggaccg cttcttcgct ccataccgcg cttccgaaac
     2761 ctgtcagctt aggatccaat gcgggcagca aagtccccac ggcgttatcc cttcccccag
     2821 gaccatcgca ggaatccgct gtggcccatg cctcacgaaa gcaactatcg ccatccccct
     2881 ggcacgtaca tcgcgcatca aggtcaaagc cgatggtttt ggcatctcct tgtgtatcac
     2941 ccaactcgac ctgatgaacg gcaaccacga aatcaatctc cccaccatcg ccatgggaag
     3001 gcgggtcggg aggttgagca ctcacgcaga tggcgtcctg tccgacatcc gatggatgga
     3061 tgtcctcccc cgtatctatc gcagcctctt gtgggatcgt cgtctcttct tgcacaacgt
     3121 cctgcacaac ctcctgcaca acgtcttgcc ccggttctag cccctcatcg tctcccgcat
     3181 cggaggtgtg atgagctgcg agcagagagg agtcgtagta cgagcaagcg ctcatgagcc
     3241 acgggagcag aaggacaaaa cgacgcacaa ccaaagccta ccctgaagca agtgcttgtg
     3301 gccatacgag ccaaccggaa agcatattgg tcgcgaaaaa ggcacagcac caggggctcg
     3361 cggcaccaac ccggcgccaa tcggccccga aagccgtcgg atgattctga ttcagcagac
     3421 cccaatatgg caattcagcg cacgggcaag tttcgcagcg atttcgagta tcaggctcac
     3481 ggcctctccc gagcttgctt tccatccgag gacctggagt gcggttccgg ctgcgtccaa
     3541 agcacaatcc gcattgttct cgggcttcgg tagccaatcg cgaccattcg caggtcgggc
     3601 gttttggctt tctcgctcag agtcgagctg gcgcttgccg tagcaaaaag tggggtaaaa
     3661 ctaccgatgc agtgttctaa gagccgagac gtttgatggc ttcgccatag gcatctgaag
     3721 ctacgaagac gatgaggtct cgcgcttcgg cgttgcgtcc gatccaattc attctatctt
     3781 ttccggaggc tggaggtccc gaggggtgtc ctccggacca ggcgatggct ttgagggcta
     3841 cggaaaggcc accttgcgcg agcagcgcgg cccggtcgcc ccatccattg atggcgatat
     3901 tgagggtaga ggcgcgatta tcgagagcac cacccacttc aagagcgaga gcggtcatat
     3961 ccggatcgac ggtggccggt ttggcggcgc tgagtttttg tgtggcctcg gcgaagcgcc
     4021 gcgcgtcgat ggtcaccggt tggaaggagg gagccagtgt ttttacgagt gccgcgaccg
     4081 taggcacaag gtcgatcggt gcggttcggg ccaacacgct tcctctggct tgcacgattt
     4141 ttagggctcg gatgagcagg aagtcacgaa cggcatgctc atcggtggtg agcatggccg
     4201 atcccaacac cacgatggga ggattggatt ggatcgggac acaggtaggt ccgagagcgg
     4261 gagatacgaa tacttgtaga ttgtgaactc ccatgctgct tgcagccgct acgatcgcct
     4321 cttgcatttg tgtggaggac ggtggaacag cggaggcccg gagggctttc aagtccatgg
     4381 ggaaagcggt ttcgaaaagg tctcccgctc gtttgagcag cgctcgcaac gctggagtca
     4441 gcacctcggg ggccaagata tcatcgagat tgacggaaca agcgctaggg ccaaaggagg
     4501 aaagttggac ttcccttgtt ccttcgagca catcgacgac tgcgcgtgcg accgctgccc
     4561 ctgcctcatc gtcgcgaatg gatgcaaccg tttccagggt ggcaaatagg tcaggactga
     4621 atcttccggt tcgaagagcc cgagccgtat ctttggaagc tcgatcgagc agcaattcca
     4681 gggatttttc gtgaccttgt ctgcgatgga attcggccaa ggcgcgaagg ggagaggcag
     4741 cggaaggcgc ttgtttgtag agacgatcga ggatttcctg agctttgttc gcgtttcctt
     4801 cgagttgctc atgaatgagc gcgagccgaa tcgtttgctc tcttttggca tctgggcttt
     4861 gcgcttgatt gacaagctgc gtgcccaatt cgatggcttt ggaagcatcg cccgtggctc
     4921 gaaacacatc aatcaagcgt tcctgcgcct ggatgtcctc ggggttgcgt ttgagcacct
     4981 cccgcaaaca cgattctgct cgttccagat tggggagatg gtccagataa atggcaccga
     5041 gttttcggta gatttcccct tgttgctgaa cgttggacac gaggcgcgtg agacggagca
     5101 aagcctgttc ggcgccgccc cagtcttctt ccatcgcggc cgtaagagcg aacgcttcca
     5161 gcgcgggttg atgatccgga ttggcttcca acgccgcggc aagtgctctt tttgccccac
     5221 cgatctctcc cacttccgcc agcactttgc cacgaaggac ctctatctcc actcgctcct
     5281 gtgggtctgt cgtggcttcg agccgccgtt cgagcagcaa tgcaagctgg gcggcatctt
     5341 tttcttcggt gtaaagcacg cgaaggttat cgaatacatc ccgatacccg atatcgatgt
     5401 cggatgcttc ttcgaaagcc ctgcgccccc gttctcgctc ctggactttg tcaatccaca
     5461 tttttgccgc ccgataccac aattcggccc ggtgcatgtc cacgttgctt tttcgagcaa
     5521 ggctctccag ttcctctgcc gctcgggcgt actcccccgt tttttccagc acgtcgacca
     5581 actccagatg agcttggaaa aaacccggtt cgcgatcgat tgccatttgg aatagctggg
     5641 aagcttcttc ttgttcatcc atgcgcgtga gcgcttgtgc cgcatggacg agcagagcgg
     5701 cgctttcgat ttcgttgtca gacaatcccg acagggtttt ggcagtctgc acgacgatcg
     5761 catcgtcgcc ctgttcacgg gcatgggcaa aggagctgcg aagagcccaa agggagggat
     5821 tcggctgttg tgccgcggca cgagcgaggt ccgccaagtc ggtccacgat gattcccgca
     5881 agcggatacg agaagcgacg acagcatgcg cgtcgacctc gggaccttgc agtgctctga
     5941 ccaattcgct agcgatgggc tcgagatcgc catctcgccc cattccgatg aatgcgtgct
     6001 caatgcgccg caaactcaac aggtgttggg ggggttgctc gagaatggtt cgatgccaca
     6061 gcagcgcgct ggccatatcg ccgcgaccga cttcatcgag ttccgcgagt tgttcgagca
     6121 cctcgcgacg cttttccatg ggcagttctt cttggcgaac ctggctcatg agcaagtcgg
     6181 tgaggtgagt ggtggaagcc cccgccaact cgcagcgttc tatgcagagg agcgctagtt
     6241 cgccagcgcc acagtccaac gcttgggagg ccaatttcgc ggcatcatcg acgtgagctt
     6301 ggcgctcact ttccagtgcg gcttccaaca acaggatggc tttttccatt ccttcggact
     6361 cgttagctcg gtccaaccgc caagcgatcc aatcttctgg gcgttcttca ctgaaccgct
     6421 cgtacaaagc ccgaagcgcc gcgtctttag gccttgcccg acatgctcgt tccatcaatg
     6481 cgacagccag agaagtatcg cgttcgacca tcaacaaagc ttcgcgacaa gcatccaagc
     6541 ttgcttcaat cgggtcgttc gtcgtttccc gacgtacccg aagccactcg atcaccccat
     6601 cggcatcgcc ccgatgtcga gcgtattgtt ccgcaaggct cgccgcatac ggtagggatg
     6661 ggtctttgtc gtgggataac cgcagcaggt cgatgtaatc atcttctccg ggccctcgaa
     6721 gcaacgctgc ttcgagggcg taggcggcgc ctttcgcacc ttcggcccgc gcggcaagtt
     6781 ccacgacacg gtttgcgtgt aggtcgggtt ccatcgaacg caacgcgcga acggcagctt
     6841 cgaagctggg ctccatggag agcactcggg cgtattcctg cgacgctctt tccgaatctc
     6901 ccaaggattc cgcgatgata gctgcgacca gggcgttgtc tcgttctcga atatggtctc
     6961 caccccagga ggcgatatgg tcgatgatgc tatcggtaaa ccccttttcg attgccattt
     7021 caagttcgag caaccgcgcc acggcgccgt tgaaagacga atcacgtaga gctgccacac
     7081 gctgccaaaa ctcatcggag tccaagcgca gagcagcggc gcgtcccacc gcggacaagc
     7141 atcggagcgg caaagctccc atcgagcacg catcgatacg ggaagcatca cgcacgtcag
     7201 tgagcgccga tcgcgctgcg gccccgagcg cagccaagtt ctgttggccg agaagtgtcg
     7261 aaaccaaagg ttccaccggc ccgtacttct cttgaaaaag agcggcaagg acgacacggt
     7321 cggcaggaga aaatgcgttg gatccaggtt ccgccaaagc gcggtccaac gcatttcggt
     7381 ctgcggattc cacggctcgc atcgcaagca tgcgcaacgc aaccgcgtga tgaggtccgg
     7441 tggcgagttt gtccaaccag ttcatcgctt gaggacgaga atcctggtgc tggcttgcga
     7501 gggcggcagc aagccataac gcgccgcctc ccataccccg aacatcgtcc aaagcgcgaa
     7561 gcgcccgggc tgctgctttg gtatccccct tacgtaacgc ggctttggct ctgggcaagg
     7621 cctcgtaggc ctgcaccgtt tctccgggct ccgcgggagc tttttctgct tctcgaatgc
     7681 gggcgacaag agccgccccc tgctccaacg acgcgagagg aggaacatcc ggccatcgtg
     7741 gcggttcctt ctcttgcgtt tcgtcattga gatagtggat caatttggtt agatgcggac
     7801 gcggatctga cggtacgatg cgggctgcca agtcgatctg cttcgcactc acgtcatcgt
     7861 ccaaatgaag ctgtgcgttc aagtgcgcgt ccatcagcaa tgcatggact ttggcttcgg
     7921 gggtcggagc cgttcgcgct tccgtttcca actcattttt cacgtcggcc caacggcgct
     7981 ctcgattggc aaggtaacgc gtttggcgct gaagcagtgg atggtcttta tccatgtcgg
     8041 aagctgcttt agcgaccgcc acagcttttt cgtcatctcc caccatcgcg cacaattcag
     8101 aaatcgcgag cataatccgg gctctttgct caccgatgtc cctggcggca gcttcttcgg
     8161 caaaccaagc cgcccgcgcc tcccattccg ttcgcatttc ccgttccatg agatgagccg
     8221 aggcgtctcg ctcgtcgggc catggatttt tcggaggctc caacggaggc ggagatacgg
     8281 agcgaaccag cgccgattct tgggcactat cagcacttgc aagctgctct gaacgcgacg
     8341 tcgccggtga gtccgaggtc acggtgattc cttggcctcg aaatacttcc gtgacttcgt
     8401 cttggggata cttgcggtct tcgggttcca gcagtttggg accggcggat accggctcgt
     8461 caagctcgtt tggttgcagc cccggaaggg gagtatctac agccgctgga accggtgttg
     8521 catccgcggg cgccggagaa ataaggtcgt cgcgcaagtc ggcaaaagga tcgaacgaat
     8581 gagacaggtc cgcgtcgttt gcctcgtcgg ttcttgaagc caaggtccgt aaggaaaacg
     8641 gctccgaact ggatgtcggg gcggatgcgg gctgttgtgc cacttcatct tcggctggaa
     8701 tggtaggttg ggaaccgcgg gaaaaaaggc tagaaagtcc tgaagcaccg cccagaccgc
     8761 gaactgaatc cgcgagcaac tccgctggca cctgtcccac caccgtgcat tcgtcatcat
     8821 catcttcttc ttgtgaaacg agatgaccag cgggtggctt cagcgtagcg ggagcatctc
     8881 ctgcactttc agaggcctct gttccaggcg gaggcggtgt tttgtcatcg ggtaaggacg
     8941 gattcaaggg agactcgtcc caagcctcga gcgcactgtc ccaatctcct gccatttgcc
     9001 catttttgtt gtcgctgtcg gtactcaccg gacccccatg cccaattgat tgcgcaactc
     9061 gagatatcgg tcgctcaaca cgaaagcgac aagctcacga gcacgctggt cctctgccac
     9121 gatcgcttgc aattcgttac gcgggacacc gaatacctcc gccaaaacca aggatacgtc
     9181 gcctgccgca atggctgcca tccggtccat gctcgacagg gcgaagcgca cccatacaat
     9241 cggatcgatc tgggaagctg ccactgcttg acaaatctcc tgcaacacgc ttttcaatcg
     9301 tcgaggtaac gccttgccaa tcagccgttg ggtttcagcg accatggcat aaggcggagc
     9361 cgcaatcggc gttcccacga gattgcagac cgcaatcaca atggatgcca ccgtgggcgc
     9421 atcgcgtgtg cgaagaacgg aaatccctct tcgaatcgcg aaaagctcgc gggcgacggc
     9481 ctgacgcgcg tggggcgcaa ggggggccga tatctcattg cctacgacca ggaagggttt
     9541 ttctcctggc acaccggtga cgccataagg ttctctgcct ccgacataaa ggtcgaaatc
     9601 gctcaaaccc agcgccccag cccaatgcgc gatttcattc ctcaacggaa gaccatctcg
     9661 tggatctaca cgatttttct tggtgacgga cagtccgacc agggtaggcc cgagcgcttc
     9721 gctgatcacg ggggccaact gttggaacaa agcgggcaaa gcaccgacat cacgagggtc
     9781 cgcgatcgcg gcaatcaccg catcatcgag ggctacctgc ggcgtgcgcg ctactcgacc
     9841 gtcaagactc tcgagttcct gaagcgcatc gtcgcccccc cgccccatgg caaccatcac
     9901 ccccagacag gcctgacgac gttgcaggtc tccttgttct ttcgaaatgc gggcaagcaa
     9961 caccacatcg ctgggttctg gagccatgga gactcgccgc accaaggtta gacgcgcttt
    10021 gctcagcaac gaggctcgca actgcccatc gcccacatga ggatggtcca tgagaaattg
    10081 catggcctcg gcatcggcgg gggattcgga cagcagttga acgaccgacg gcaacgctcc
    10141 actggggtcg ccggttttgt ctcgatggat gaccatcgcc aaccgcgctg cctgaattcg
    10201 tcccggcgcg ttttcccgct cgtgcatcaa cgtctcgagc accgcggtag cctgccccca
    10261 agcctcgttc gtagccgcgg ctttcgccaa ccgctcacgc acgggcagcg tggacaagcc
    10321 ggtggtgtga agcgtcatca gtatttcgaa ggaccgtttg tggtccccca atttgttttc
    10381 gcacaagtcc acggccgcca tgccggagac aagacgctgc tgagcgggcg cttctttatg
    10441 agctgccaaa cgagccaacg catcgaccgc ctccccaaac gccccttgtc ggatatacac
    10501 ttctcccgat agggcgagcg ccccgacatg atctggctcc atcagggtta cgttttccaa
    10561 agcggccatg gccgcggcaa aatcgccttt ttgacgaaga acgcgcgctt gttcccagaa
    10621 cattttggcg atttcctcag ggtcttcggc gaattccagg cgacgcgcga tgagggtaag
    10681 caagtagtca ttgtcttcac gggctctgac tcttcgaaaa agtctgtcga aacagacgaa
    10741 ccgatgcccg tcccgctgaa agccttttgc cagcgctagt tcaccacgct cctgatcatc
    10801 gcctcggtcg atccacaaca accctgccgt ttcaaaaaac tccgctgctc gggcatcgtt
    10861 ggagcaaagc gcgcccaagc gctcgcaagc cgtcgcagtc caccgaacgt catccaccat
    10921 gcttgcggca gcacggacac cttcccatgc cacaatatcg tcgtcccgag ccgcacaaac
    10981 acgacgaaaa acatctaagg cctgttcccc ttcgcccgcg gcaagcagcg accatcctgc
    11041 aagcatacgg gcgtcgattt tagcgcgatt gtcgagagcg tcgttcagtc cgagcaatgc
    11101 tttcgcgcgt tttctcgggt cacatccagc cggcgccaac tccaagttca tgagttgtgc
    11161 gggcggttcc gagctatcga gcaagggtgg agagtccgaa ggggtcgaac gagtgagatc
    11221 gatcaacgat gcacttgctt ccaaagcact cgccgtttct ttttccacgt gacgagcaag
    11281 aagacgtcga gccgagcttt cgctagcttc gtcttccgcg gcacaagacg ccacgagcca
    11341 ctcgatggcc ggcaccacac cgccatcggc catggcccaa gcctgcgcca agtcacgagc
    11401 tgcatcacgg tctttttcga cgctacgagc gaagcggtat cgttctgccg ccacgaccgc
    11461 cgaagcacga agtcccagtg tttcgagccc acccatggct ctttcgcgcg cggtagcatc
    11521 ttcgaattca ggagcatgca ccaaacgccc cagccatcca gcgacaccaa tttcccccaa
    11581 cgcatcccgt tcgagcgcgt ccaacgcggc agaagcctgg tcgtcgtcgc ctccctcggc
    11641 cgattcgacc gcgaaacgct ccaacgccaa agacactttg tccgcgccgc attcctcgtt
    11701 caagtccagc atacgacgtc gtccatcgat ggtttccgta tcgagggcga cagtcgccca
    11761 caaaagcgcg gcagcggcgg cctcgggcag caggtcacga gcagcgcgaa tcgattgtaa
    11821 ggcagcgttt ttgtctttcg ctttccaaaa taggaaagct gcttcgaggt gcaacgcggc
    11881 cgccaactcc gcatcttcga gactggtagc cgatgtgctc acgacccgcg ctgctgcttg
    11941 tgcgtcgtcc gacattcgca acaaatccgc gagaagcacg cttacgagca agtcactgtt
    12001 atcctgctca tgcaataccg atagcgtttt catcgctcgt tgcgagtccc ccgcgcgggc
    12061 acggagcagt tgtgccacga cacgcaaggc acgagcgatg gcagggtcct cctcgacgtc
    12121 cgcgagagct tcgattcggt ccggggagcg cgaagctttc gaatcctcag catcgagttt
    12181 ggaaagaccg agcgcataag cagcgagcat tcggcctagc catgctccgg cttgtacgtt
    12241 cgacaacgaa tcgagtgctt tttctgtttc cgggtctttt cgttggagcc gcagccgcgc
    12301 gagttcaaac cacaacgaag catgctcttc cgcccccgac cccgcagcta tcaaacgtct
    12361 ggtcgagtct tcataccaac tatcatcttc gagaatggaa gagaaagccc ttgccaaacg
    12421 agatacgaca ccgtgctcga ctccgaacat ggcggcttga gacatagccg ccttggcccc
    12481 tgacacatca ttcgccaaaa tcgcccatgc ccaagccgca agaagataag agcgcccttt
    12541 ggcttcgtca gacaagccgt gttccgccat cgcctcgagc gatgcagcaa aagcagtggg
    12601 gtcgctgtcg agcagcaggt ccgtttgcaa agcccttgcc gggatacagc cttgatccga
    12661 cgacaatgca ttggttagcg cttcgagcgc cgcttctcgt tctccacgag cggcagcgcc
    12721 ctcgaatatg cgcagccaca aggccgcagc caaaggccca gagcgtagcg gcagcaatcg
    12781 ttgcgcaagg gaagccgcgg tttgagcatc acctgctttt tcggcagctt gcattcgcgc
    12841 aaggagcaca acgccatcct cggggagttg tgacaccgca aggtccaaca acgatgttgc
    12901 cgcatcgata tcacctgaac gctgagctgc ctgcgcggca cgaaggaaca aactcgccac
    12961 atgggcagat gttcgaacat gggaaggaac tgcatccaca cccaccgatt catcctgcat
    13021 ggccgccaag atgatgctgg cttgtttttt caacgcctcg gcgatcaaat cctctcgttg
    13081 ctcgcgtcgg gccaaacgct cgagcaaaag ggctgcacga aatcggtggg gagaatccaa
    13141 ttcttcgacg gcatggatgt ggtcgagggc tttgtccccg ttgccgcgtt cgtgctcttc
    13201 tttcgccaag tccagcagca gcaaagcctt ccaagccgtc ggctcggcca atcgaacgcg
    13261 ttcttccatc gcccgaatgc gcaggtcctt gtcttccgtc tttccactca cgatttcgag
    13321 ttcgagccat gcggcagcaa ggtccggatc atccgtcacc gcttgttcga aagcgtcttt
    13381 cgctcgtacg gggtcgcttt cgtgctccaa cagacaggaa ccttgcagca aggttgcgcg
    13441 tgcgatttgc gggggagtcg ttgccgcgcg caccagcgca tcgaatagtt ttcctagatt
    13501 tttgaaggat tttcgacgtt ccaacaacga agcgagggac tcgatgggtt cacgaaaagc
    13561 gggattcgca ttgtaggaag ccaagtaatc cctggctgct cccgcttcgt cgcgaatacg
    13621 ctctcgcgcc actgctgact caaaaaaaag caatgcacgc tgttcgtccg tggtcgccac
    13681 ctcctgctca cgaacgagcc aatcgatgtg ccgttggatg acctctggca cctcgaccga
    13741 aggggaagca gcaggagccg ttgatccaga tgagttcgcg tcggaagaag tcatgcgatc
    13801 cgtgttacct ccgtgcgatg caggttgagc acgcgagaca ccacacgagt gtaggctagg
    13861 atgcttgcgc tgcctagaca gtgtcgatgc tgtcgcggaa aagccgtgtt tcgcaagggg
    13921 aaaacacgtt ttccacgaga agctttcact tattctttcc gatatttttc acgatccaat
    13981 accccgtcgg agtatcgatc ggacccgcca cttcgccggg gggcgtcgta aataacacgt
    14041 actccggcgc aggttccaac acgcgtcgcg gcatccatcc cgccgcggcc gtcgagcccg
    14101 catccccttg agccaccgcg gccgcaaagt cgtttttcgc tacctccaaa agatgatggg
    14161 ctgttttcaa cgcctccatc ttcgaacggg caccaagggg ggcatgctcc gcacctcgat
    14221 actggacaag cacgacccca aaacgaacgg aacgcggagc ttccggcggc aaatccggcg
    14281 gtttgctccc atcgagcatc acataccccg gctttgcccc acgaactccc gaaaaggaag
    14341 cctcttgaac catgtccgga accgatccag cgtccaagga cgtcattggc tcgacttgtt
    14401 ccggcaccga cgacgataat gccggaattt gtaccgcggg tgaagcgcgg ggtcgccccc
    14461 cccaaacgac gagtcccgtc acgacaagca ccaaggcacc aagaaaaacg gaggtcgtct
    14521 tttgcatgaa ttccttgcct atcgcagatc ccccggatag gggcaaggac gaggtgggaa
    14581 tgacatacgc atgcttggcg taccggaccc aagttgtgcc acaatgcccc cacggtgacc
    14641 ttttacccgt tgcctagctg actcatgctc aaagtcgaat gcgaagggtg ccacgcccca
    14701 tatcgagtag atgaacgacg cgttcccccc acggggctca agatgcgttg tccgaagtgc
    14761 gggaaaacgt ttgtggtgaa caaggagtcg ccttctccgg ccgcagcacc accagcacca
    14821 ggaaaggtac cccctcctcc tccttccctt cgttccagcg ctgcaaaacg caccatgttg
    14881 ggcgttgggt ctcctatcat cctgccacga ggagcagacc caaacgcgtt gcctccacca
    14941 ccggatgact tcgatatcgg cttgccggtc gtatcgcccc ccgtcggcaa atccgcttcc
    15001 aaagccgctg cccccgtgca tgccgatctg cccagcgctg ccaaacctct cccgcccttc
    15061 cctcccgcag ctcgtccggc cgttgcacaa cgacctgcac cgcccgcccc tgccgccgcc
    15121 ccgtccaccg ccaatgacga tgatttggcg gacttcgggg atcttccttt gcctgtcatt
    15181 tccgcttccc ccaaagaagc ggcgctcaaa gatttgcccg cgacacgcgc gtcgaaaacc
    15241 gcacctttcc cccaccttcc cgcaaaagca cctgcttcac ccgccgattt tggcgactta
    15301 ccttccccga ttggttcgcc caccgacttc ggcgatttgc cttccccagt agcttcaccc
    15361 gctggttttg gcgacttgcc ctccccagtg gcttcacccg ctggttttgg cgacttgcct
    15421 tccccagtca tcgatctatc gcgaccggac gcgggactac cagtcgtcag caccgatcag
    15481 aatcgacccg cgcgtcagat tacgggcgct gcccttggat ttgggggtcc cgatcttccc
    15541 tccccgagga cttccgtatc gaatgaagcg ggatttgggg atggttttgg tgaactcgat
    15601 cttcccctcg cggctaccgt tggccttccg acagtagcct ccagtcaagc agaattaccg
    15661 tctccttccg atctatttgg aaaccttcct tcgacggccg atccgaacgc caattttccc
    15721 acacccctcg ggttgaatca gcaactgccg tcacggcaag ggacaccatc gaacgcatct
    15781 gtcgatgatt tattcggtcc gacgccacca tcgccggggg gattgttccc ggacggtatg
    15841 ccgccggccc ctcctccatc gcaagcaccc acggggcttg ggtctttccc cccgatcgga
    15901 acgggcggat tatcgtcgcc accggatggt gcgatttcgg gccaggccgc gggctctctg
    15961 gcatcccagg ccgatgcttt tgggtcgcta ccaccggcgg ctcccgcggg tcaggccacg
    16021 attcaacgtc aagcaggcgg cggagttgcc ttcggggagg tcaatctgac cgacgatggt
    16081 cagggtgctt cggcgaccac cggcagtctt gctccggggg cggggacgag ttcttatgga
    16141 gaaaggagtc ttgggggtgg ggatgaagac atggagtttg gagccattcc gcaagaggat
    16201 ggcgaggata aaagcatcga tgcggcccca acggcgcttc ccaccaaagc cgaggttggc
    16261 gtcgtcgccc ttgctccccg gaagtcgaag gcgttgaaaa tcattcttgc cgcgattctg
    16321 gtggggctta cgggcggaat cgcgatgtcg gcaacgccgc tgggcgcatt tggcacgcac
    16381 ttcctaagcg acatgctcaa ttccggtcga aatacggcca tgctcgatga aacggtgcag
    16441 aagtcacgta cggcattgtc aatcgacacg gcagaccagg cccaagcatg cattgagcaa
    16501 accgacgaag cggtcaaccg tgcgccacga atggcctcgt tgtcagcgta tgcagcgttc
    16561 attggatttc aatgggagct tcgttttggt tccgattcgg cggtccatgc gcacgccacg
    16621 gcgctgctcg cgcgagccat cgaaatcgac aaggaagctc agcacttgga acttgctcgc
    16681 gccgcgcaag cttcatcgga aggggagatt gcaaaagctc gacaagccat tcaagaattg
    16741 gttcggctcg acagcaacaa catcgatgcg gcggtgttgg aaggcgaact ggagctacgc
    16801 gcgaaggaat tcaacaacgc gctcgtagcc tggcagcgag ccgcgggcct tgaaaaatcc
    16861 gctcgcacct cgtttgggca agcgcgtgc
//
LOCUS       DAOSOR010000044        16705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_3476_length_16705_cov_4.840420, whole genome shotgun sequence.
ACCESSION   DAOSOR010000044 DAOSOR010000000
VERSION     DAOSOR010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 16705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16705
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3476_length_16705_cov_4.840420"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PK710_04000"
     CDS             complement(<1..204)
                     /locus_tag="PK710_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08906.1"
                     /translation="MRSKAVFVVLLLMLLGFVASTLVRWRMPTFIEMATPTASSATAV
                     VSSNFAPNIAPNASSAPKMPVASS"
     gene            complement(209..1450)
                     /locus_tag="PK710_04005"
     CDS             complement(209..1450)
                     /locus_tag="PK710_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOT08907.1"
                     /translation="MARTPFAPKSSKFSVAHGILKTITIIVGLMLSQACLMAVVGMLT
                     GNGWIRGLVALVINLVVPLVVVDRVLPNDDPTRAKGLASDVLALIWFGFPFLFMVAGH
                     SLTRGPLAREAIRYTDAGFGTLGRVTLWLSGSAAVASESDSPGVVASATPSPTPSSTA
                     SAALSASASASSPVPPPPASSASSKPPQPPPIIKDAGPQEKSPAQLFKELSPAVVTIS
                     VANAANLEGGGTGFLIDTQGTIATNHHVIGGAQKISIKFLNGAKYQTVELLAEDSSRD
                     LALLRVNLDTPTEGKKGEVIPLVLGNSDTVEVGERAISIGNPLGLEHTLTDGLVSARR
                     IYEGKTWIQMSVPVSPGNSGGPLFNMRGEVIGVTTAQVMGGLGGRAQNLNLAVPVNAL
                     KNMIQSGFPNRRTFGDGSSQW"
     gene            complement(1618..2847)
                     /locus_tag="PK710_04010"
     CDS             complement(1618..2847)
                     /locus_tag="PK710_04010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT08908.1"
                     /translation="MRVLAITNLYPNPYQPHRATFKRHQLRILGTRVPVEVIAPISWT
                     DELRARRMGKAPLPPNRKLNHDGLTVFHPRYYFSPRIARRLYGHFYMHSISRIFFKEI
                     EDFRPDIVFASWAYPDGWAAVQLARAANLPVVLQVHGSDVKLVDHYPSRRRGTIQALT
                     GADGVIAVSQDLANSAVHLGVKPGKVRVIYDGIDLAHFRPGDKASARTFLNLPPSEEP
                     ILLFIGNLLHVKAVDILLPAVAKVVNSGLPVQLYLIGDGPLRESLQQLAKDLGLESRA
                     HFLGAIDQFRLPRWYQAADLFVLPSRSEGVPNVLLEASACNLPWVASRVGGIPEILHR
                     GKGFLVPPEDVDELAEGISTWLKVAAHKATTGKRRLSVPPCFVPPKTREQAVGEVIEF
                     LSQFIQNSSRLTKPYPF"
     gene            complement(2847..4007)
                     /locus_tag="PK710_04015"
     CDS             complement(2847..4007)
                     /locus_tag="PK710_04015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT08909.1"
                     /translation="MNDSVSQSLHVTHAVLSLDVGGLERIVLDLARIGVQRGDRVTVI
                     CLEKQGELAPIAQRFGAEIHCLDKPAGRHLEIIPKAASFLQRARPQIIHTHQIGPAWY
                     LGQVAASFGIPLIHTEHGDPFARAKRLWNIAKLRWLYRQSSRWLRRFCCVSHPIAQTM
                     RRFHTVPNSLLTVVPNGIRTDAPPQMPSRSQIRAQWSIPQDVPVIGTVGRLDEVKRQD
                     LLIDAFARIAPDHPCHLLLVGDGPERNKLEQRSRSWGLESRIHFTGFQTQPEAFYRAM
                     DIFALTSRSEGLPVSLLEAWSAEKPAVCTAVGGIPSILTHGVHGRLVPSGNVEAMANA
                     LRWCLQKPQKAMDMGRRGRAVVLAQYSLDHMADTYREHYLHAFHPKPDRGER"
     gene            complement(4004..5314)
                     /locus_tag="PK710_04020"
     CDS             complement(4004..5314)
                     /locus_tag="PK710_04020"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOT08910.1"
                     /translation="MKISVLGLGYVGCVSAACFAKLGHEVIGADVNQVKVDIISSGKS
                     PIVEPGIEEYIAETVKAGKLRATTDAVAAVLESDVSLVCVGTPGQHNGSLDLTYIRRV
                     CEHIGEALAKKSTYHVVAMRSTMLPGTIHETVIPTLEKVSGKKAGKDFGVVINPEFLR
                     ESTSIYDFDNPPFTLIGADDNAAAAPLEQLYAHLTAPLFVVKVREAEMVKYACNAFHA
                     VKVVFANEIGNICKMLGVDSHPVMDVFCKDTKLNLSPYYLKPGFAFGGSCLPKDVRAI
                     TYKAKSVDVDVPLLNSLNPSNRKQIEIAIDRVLQTGKKNVGILGMSFKSGTDDLRESP
                     MVSLIEALIGKGIKLSIFDRDVQLARLFGANKGYIEKEIPHISSLMGTSLADVVAGAQ
                     VIVVGKKEKEYAEALQHVGSDVTIIDLVRLPGIEHLAGYDGLSW"
     gene            complement(5324..6568)
                     /locus_tag="PK710_04025"
     CDS             complement(5324..6568)
                     /locus_tag="PK710_04025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT08911.1"
                     /translation="MKVLWVKSELLHPVDKGGKIRTFEMLRHLMRSHEVTYLCLSSPT
                     DAADARERASEYCHHLQTVPWSEPKRFSTGFYVDLAKNLASPLPYVIQKYKQPQMRQF
                     LARSDARREFDVVVCDFLTPSANVPRRLHAATVLFEHNVETVLWERTFQNEKNPVKKG
                     YFFGQAVKMRAYEHLLCKRYDAVAAVSEPDAQAIRQRFGVKDVYAVPTGVDFDFFSPL
                     PQDHALKLAREPFDLVFTGSMDWMPNEDAILFFADEILPRIAAEIPQVRVTVVGRNPT
                     PTLQALAKREPKITLTGRVDDVRPYIDRSAVYIVPLRVGGGTRIKIYEAMGMQRAVVS
                     TTIGAEGLPVENGKEIVLADDPAHFAQQVIRLIHDTEAREAIEIAARKAVVERFGWDR
                     ATDALVDIMERARNKRARRHHR"
     gene            complement(6665..8218)
                     /locus_tag="PK710_04030"
     CDS             complement(6665..8218)
                     /locus_tag="PK710_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOT08912.1"
                     /translation="MLHAIPQLQDYLIHSAARLGDKIALVCDKKRYTYAELDAQSNAL
                     AHTLVQQGVTRGDRVVVFADNSVETVIAFWAVLKANAVVSVVNPLTKTDKLRYLLEDC
                     RATALIADGLLSRVLAPVVADNKHLKVLIMTGKPLSPTPENLQVMTFDQALSSGNPRV
                     PPARTGIDIDLAAIIYTSGSTGDPKGVMLTHRNMLTAGLSVSTYLGMQEDDVVVCVLP
                     LSFDYGLYQMLMSFRVGARLVLERSFTYPAQVLKTMVEEKVTGFPGVPTIFAILSENK
                     NLRDYDLSSIRFVTNTAAALPVKHIEMLLDLFPQARIFSMYGLTECKRCTYLPPEDIR
                     RKMTSVGIAIPNTEMWIVDEQDRKVGPNEVGQLVIRGATVMRGYWEKPEQTAKKLRPG
                     PLPGEYVLYTGDYCKMDEEGYLYFVGRMDDVIKSRGEKVAPKEVENALVHIPGVKEVA
                     VIGVPDEIFGQAVKAFVVPEQGAELTEKLILTEAKARLEAFMVPKYVQIVPDLPKTTT
                     GKIKKTDLR"
     gene            complement(8396..10231)
                     /gene="pheT"
                     /locus_tag="PK710_04035"
     CDS             complement(8396..10231)
                     /gene="pheT"
                     /locus_tag="PK710_04035"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOT08913.1"
                     /translation="MPIVNIDASWLNELLEKPYPPQQLIDALDQIGCDVEEVVELPRY
                     RCPQCQALVEGSLGADTVKQCSLCGFTQMQPFEIVDTITAIRLDLLAARPDLFDIGGL
                     VRALQGYLGEKKGLPSFTSTRSDIIVNVDPSVCQPNSYRPFLRCAVIELPSVDDRMLV
                     ALMKLQENLHWGVGRDRKLASIGIYDLDTIKGPITYKTLHPDDEPFVPLGVSEPMSGR
                     KILSEHSKGIAYAHLLANHDRYPVLVDADGHVLSMPPIINSERTKIKIGSHRLFVDVT
                     GISIAAVENTLHTLVSSLVELGATIRTVRIVQDEKELETPDLTPKQAIVDLSQAKQWL
                     GLPLDAHTLMESLFRMRLDVEPIDESKTRFRVRYPAFRSDIRHTVDVFEDVAIGFGFQ
                     NIQAKLVPTMTVGSSRTEEQTSDLARTILLGLGYSEIMSLPMTTESDHYERFRMPVPN
                     RYPRVGNPKLKALTVVRQHLMSGVMQALHDNRRRPMPQRLFEIDNVALLDDEAPTGCR
                     EERRVCFVEMGKDAGYATARSILDAMLREMGVEAAYSATQQPWFTAGRAARFQAGSFE
                     GILGELHPEVILAFGLDHPVALVEVTLGSLRSWPIPHPRTTERSS"
     gene            complement(10222..11757)
                     /locus_tag="PK710_04040"
     CDS             complement(10222..11757)
                     /locus_tag="PK710_04040"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF003210.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOT08914.1"
                     /translation="MIAPVVLTETAKKVWEAVRAGPKDVADVVQTTQLDQSLVMVAAL
                     EAQQQGFFDVKERVRLELDPVPDAAARLQQGLPERQALRLLIDAGGSLSVPDFTQRAQ
                     AQGLPVHEIFRWGSARGWVERTKGQPPMVRVTDAGRLAVDEPADDERALRLALEGTRF
                     LDEQALQGVDPVAVEQLLGKRTDLAKLRKRTQRILSLTTQGLEALSRAKVKREQNALA
                     PEDFQSGAWRDITLRPYDVTLPGKTVYPAKIHPLRKIMEQTRRAFLELGFEEVVSPMV
                     ESAFWNFDALFQPQDHPARDMQDTFYMLDPARIPLPPPEVVEPVRRTHEHGGDTGSTG
                     WGYKWSEREAERVVLRTHTTAATIRALAAHPNPPYKAFCVGWTYRNETISFKHLPVFH
                     QIEGIVIDEQASLATLLGTLTAFYQKMGFAKVRFKPAFYPYTEPSVDVMVYMESRGKW
                     IEMGGSGIFRPEVSEPFGCKHPVLAWGLGLERLAMLRLGFSDIRQLYQGSLDAMEEVA
                     LCR"
     gene            11902..12426
                     /locus_tag="PK710_04045"
     CDS             11902..12426
                     /locus_tag="PK710_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOT08915.1"
                     /translation="MTRIRVVGRDQPIEAPEGSSLLAALLAARSPISTSCGGQATCGL
                     CRLTVLQGSSLLSPVNAKEVLHLGNVAKVVSIRLACQALIMGEGEIEIDVPAVVDVAA
                     RKREQARRGFAERAQRRRSVPGTQASPETPLEPAPKAAVERIEWRPRMLNPGPEQTPD
                     PDLPADRKQKSQRS"
     gene            12779..13144
                     /locus_tag="PK710_04050"
     CDS             12779..13144
                     /locus_tag="PK710_04050"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOT08916.1"
                     /translation="MRKDELEAASTLGSGISIQGRIMGEGNLTVEGSVEGDVTIQGDF
                     VVAAGARVHGSVEAHGVVVEGDLEGDILATGPVHAGAGASLRGNVKGQAFTMEEGATV
                     SVTVDADFDLPPELQGGRE"
     gene            13186..13500
                     /locus_tag="PK710_04055"
     CDS             13186..13500
                     /locus_tag="PK710_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOT08917.1"
                     /translation="MASTVIGPGITIEGEVIADDDVVVQGTIRGKLTSQDAVIIEAGA
                     TVEADVSAASVVVSGSVTGDVQAADRVDLQSTGRLVGDVKAARLTIADGASFKGNVDM
                     DV"
     gene            13578..14297
                     /locus_tag="PK710_04060"
     CDS             13578..14297
                     /locus_tag="PK710_04060"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOT08918.1"
                     /translation="MADSEAIIGQGTFIRGNLRGEGDLCVVGHVEGVIEVRGQVIFEE
                     TSIIQADVTAERIIVRGAIAGELSAKESIVLEEGCRVVGGLRAPRLRIEPGALYRGRI
                     DMMEGGAVTAAASAKQQSRPAVQATQPSAAGRSASVVASAPARPAPARPAPSRTAPNR
                     PPARPARAQAVQPLPEPEVQPQPASSVALPRVGRIRSAPAVTESAPVQASDSGDVPPP
                     PVVPALKKKTKGAVRKRGDAS"
     gene            14294..14848
                     /gene="pyrE"
                     /locus_tag="PK710_04065"
     CDS             14294..14848
                     /gene="pyrE"
                     /locus_tag="PK710_04065"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOT08919.1"
                     /translation="MNAKRERLIELLRELSFERKRVILASGRESDFFIDCKQTALTAE
                     GHVLLGELMFHALRQLAPVKAVAGVELGGCPLASAVALTSFLHGAPLVALYVRKSQKD
                     HGMKKMIEGDKSMHEGMAVALVEDVITTGGSTLRAAQVLRQAGLVPVGVVAIVDRLEG
                     GSDALREAGLPLVALTTRKDFIAD"
     gene            15235..>16705
                     /locus_tag="PK710_04070"
     CDS             15235..>16705
                     /locus_tag="PK710_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08920.1"
                     /translation="MRREFGIPFVAWVGAAVLAHLLWTTGADRVARVVEDFSRIRWFV
                     ESVSAEVARAQQVEISLEDMPLQAEDDSEQSQQERESRAKDKMEQPPSPAESEPEPPV
                     ESKPKPSDPIQLFPVPSVEAPKVQVVPEIKKDQRIAVKQHVKDKDQPDNPNARFIGDE
                     ANHVQEETVARITSHDQDDQNPTPGGQHSSEDRRPGNADETKIGDSEDREGEPDKAPG
                     EHVVEEKEGKVDEPPARTASAPSVEPKAGDPARVAARDKPMTPQPPPQPTTIPEEAEP
                     LVSGQGTYSLDPRRKKKPVDTRSPGPSRLTTPNRVGLGAGPNERGIQTNLTMDDVVAS
                     VGRDELARQRAADGERRRSQHRGSWHSSSFERWRGAIENYVSSVRPGNQTALNTARVP
                     FAAYLVQIHNRIHPVFADTFLASLDALPFDHPINDYKLMARLEIVVEPNSGRVIKMGV
                     IKTSGITAFDVGALDSVQRAGPFGKAPSGIISTDGNVYLH"
BASE COUNT         3495 a         4491 c         5165 g         3554 t
ORIGIN      
        1 ggaagaagcg accggcatct ttggcgcgga tgatgcattc ggtgcaatat tcggcgcgaa
       61 attcgacgat accaccgccg tcgccgaaga ggcagtgggc gtggccattt cgatgaacgt
      121 tggcattcgc catcgaacca gcgtcgaggc tacgaacccg aggagcatca gcaaaagaac
      181 gacgaaaaca gctttggaac gcacgatttc accattgaga ggagccgtca ccgaaggttc
      241 gtcggttagg gaaacccgat tgaatcatgt tttttagagc attgacgggg accgccaggt
      301 tgagattctg ggcgcgcccc ccaagccctc ccatgacttg cgccgtggtt acgccaatga
      361 cttcaccacg catgttgaat agcggtccgc cagaattgcc cggcgatacg ggaaccgaca
      421 tctgaatcca tgtctttccc tcgtaaatcc gacgcgccga caccagaccg tcagtgagcg
      481 tatgctcgag cccgagcgga ttgccgatcg aaatggctcg ctcccccacc tccaccgtat
      541 ccgaattgcc caacacaagg ggaatgacct cgcccttttt accttccgtg ggcgtatcca
      601 gattcacccg caaaagagcc agatccctgg acgaatcctc ggcaagcaat tccacggtct
      661 ggtatttcgc cccgttgagg aatttgatcg agatcttttg cgccccgccg atcacatggt
      721 ggttggtcgc gatggttcct tgcgtgtcaa tgagaaatcc cgtgccaccc ccttccaaat
      781 tcgcagcgtt ggccacagaa atcgtgacga ccgccgggga tagctccttg aatagttgcg
      841 cgggcgattt ttcctgagga cccgcatcct tgatgatcgg tggcggctgc ggaggcttcg
      901 aggatgcact cgacgctggt ggcggaggaa ccggcgacga tgcagatgca gatgcggata
      961 aggctgcgga agccgtcgag ctgggtgtcg gactgggcgt tgcgctcgct acgacaccgg
     1021 gcgaatccga ttccgaagcc accgcggccg aacccgacag ccacaacgtc actcgcccca
     1081 gggtgccgaa gcccgcgtcc gtgtagcgga tcgcctcccg cgccaaaggg ccccgcgtga
     1141 gggaatgccc tgccaccatg aaaagaaacg gaaacccaaa ccaaatcaac gcaagcacgt
     1201 ccgaagccag ccccttggcg cgcgtcggat cgtcgttggg aagcacccga tcgaccacaa
     1261 ccaaaggcac caccaggttg atgactagag cgacaagccc gcgaatccag ccattgcccg
     1321 taagcatgcc cacgacggcc atcaagcagg cttgggaaag catgagacca acgatgatgg
     1381 tgatcgtttt gagaatgccg tgggcgacgg aaaacttgct cgattttggc gcgaaggggg
     1441 tgcgtgccat ggtggatggc gatgttcgca ttctttgggt tgtattgaga aggggattgt
     1501 cgtgggactg acaggctttg gcttcgaatc tgtcgaaata taaaaattag taataaatac
     1561 aattgattat gttaatgccc cgaggttggg tgaaccatgg gcaagggcgt tttctcatta
     1621 gaaagggtag ggcttggtca gccgcgatga gttttggatg aattgggaga gaaactcgat
     1681 cacctcgccg acggcttgtt ctcgggtttt ggggggaacg aaacagggag gaacggataa
     1741 gcgacgcttg cctgtcgtag ctttatgagc ggcgactttg agccatgtgg agatgccttc
     1801 cgccagttcg tcgacatctt ccggggggac gagaaatcct tttcctcgat gcaagatttc
     1861 tggaattccg ccaacacggg aggcgaccca gggcagattg cacgcggatg cctcgagcag
     1921 cacgtttgga acgccctcgg agcggctggg aagcacgaac aagtccgctg cttgatacca
     1981 acggggcaga cggaattggt cgatggctcc gagaaaatgc gcccgtgatt ccaggccgag
     2041 gtctttggcc aattgctgca aagactcccg caggggccca tctccgatga gataaagctg
     2101 gacgggcaag ccgctgttca cgacttttgc caccgcgggc agcaggatgt cgacagcttt
     2161 gacgtgcagc aggttaccga tgaaaaggag gatgggttct tcggaaggcg gtaaatttag
     2221 aaaggtgcgt gcggatgcct tgtcgccggg gcgaaaatgg gccaggtcga tgccgtcgta
     2281 aatcaccctt acttttccgg gttttacacc aaggtggacc gccgaattcg ccaggtcctg
     2341 actgaccgcg atgaccccgt cggcgcccgt gagcgcctgt atcgtaccgc gacggcggga
     2401 aggataatgg tccacgagtt tgacgtcaga gccatggact tgcagtacca cgggcagatt
     2461 ggcggctcgg gccagttgca cggcggccca accatcggga taggcccagg aggcaaagac
     2521 gatatcaggc cgaaagtcct cgatttcttt gaagaaaata cgcgagatcg agtgcatata
     2581 aaagtgcccg tacagccttc ttgcgatacg gggcgagaaa taatagcggg gatgaaaaac
     2641 cgtcagacca tcgtgattga gcttgcgatt gggaggtaaa ggcgcttttc ccatgcgccg
     2701 ggcccggagt tcgtccgtcc acgaaatggg cgcgatgacc tcgacaggaa ctcgcgtacc
     2761 caggattcgc agttgatgcc gtttgaacgt cgcccggtgg ggctggtacg gattggggta
     2821 caagttggtg atggcaagga cccgcattac cgctctcctc ggtccggctt gggatggaac
     2881 gcgtgaagat agtgctcacg ataggtatcc gccatgtggt cgagtgagta ctgggcgagc
     2941 acgacggctc gtccccgacg acccatatcc atcgcctttt gcggtttttg caggcaccat
     3001 cgcaaggcgt tcgccatggc ttcgacgttt ccagagggga ccaggcgtcc atgaacgccg
     3061 tgggtcaaga tgcttgggat tcctccgacg gcagtacaaa cagcgggttt ctccgcactc
     3121 caggcttcca gcaacgacac gggaagtcct tcggagcggc tcgtgagagc aaagatgtcc
     3181 atggcgcggt agaaagcttc cggttgcgtt tgaaatcccg tgaagtggat acggctttcg
     3241 agcccccacg atctcgatcg ctgctcgagt ttgtttcgtt ccggtccgtc gcccaccaac
     3301 aaaaggtggc agggatgatc gggcgcaatg cgggcaaagg catcgatgag taggtcttgc
     3361 cgttttactt catcgagacg ccccacggtg ccgatgacgg gtacatcttg aggaatggac
     3421 cattgagcgc gaatttggct tcgtgatggc atctgtgggg gggcgtcggt tcgaatgccg
     3481 ttaggaacga ccgtgagcaa ggagttgggg acggtgtgga atcgccgcat ggtttgggcg
     3541 atcgggtgcg agacacagca aaatcgtcga agccagcgcg aggattggcg atagagccag
     3601 cgaagtttgg cgatattcca gagtcgtttt gcgcgggcga atggatcacc gtgttcggta
     3661 tggatgagag ggataccaaa agaggcggcg acttgtccga gataccaagc gggaccgatt
     3721 tggtgggtat gaatgatctg agggcgcgcc ctttgcagaa aggacgctgc ttttgggatg
     3781 atttcgagat gccggccggc aggtttgtcg agacagtgaa tctcggctcc gaatcgttgt
     3841 gcgatcgggg caagctctcc ttgtttttcg agacagatca cggtcacccg atcccctcgc
     3901 tgcacgccga tgcgcgcaag atcgagcacg atgcgttcca atccccccac atcgagagac
     3961 aagaccgcat gggtgacatg caaagactgg ctcacgctat cgttcaccaa ctaagaccat
     4021 cgtatccggc gaggtgttcg atgccgggca agcgaacgag atcgatgatg gtgacgtcgg
     4081 agccaacgtg ttgcagggct tcggcgtatt ctttttcttt ttttccgacg acgatgactt
     4141 gtgcgcccgc gaccacatcg gcgagcgacg tgcccatcag cgaggagata tggggaattt
     4201 ccttttcgat atagcctttg ttggctccga acaggcgagc gagctgcaca tcgcggtcga
     4261 agatggatag tttgattcct tttccgatga gcgcttcgat gaggctgacc atggggcttt
     4321 cgcgcagatc gtcggtgccg cttttgaaac tcatccccaa gatgccgacg tttttctttc
     4381 cggtttgcag aacccggtcg atggcgattt caatttgttt tcggttcgac gggttcagag
     4441 agttcagaag gggaacgtcg acatcgacgc ttttggcttt gtaggtgatg gcccgaacgt
     4501 ctttgggcag gcacgatccg ccgaacgcga aaccgggctt gaggtaatac ggggagaggt
     4561 tgagcttggt atctttgcag aacacgtcca tgaccggatg gctatctacg ccgagcattt
     4621 tgcagatgtt gccgatctcg ttggcgaaga ccactttgac ggcgtgaaag gcattgcatg
     4681 cgtatttgac catttctgct tcgcggactt tgacgacgaa caagggagcg gtcaggtggg
     4741 cgtagagctg ttcgagggga gcggcggctg cgttgtcatc cgcgccgatc aaggtgaagg
     4801 gtgggttgtc gaagtcataa atcgaggtac tttcacgaag gaactcggga ttgatgacaa
     4861 cgccgaagtc ttttcctgct tttttgccgg acactttttc gagcgtgggg atgacggttt
     4921 catggatcgt gccgggcagc atggtgcttc gcatggcaac gacgtgatag gtggattttt
     4981 tggcgagcgc ttcgccaata tgctcgcaga ctcttcgaat gtaggtaagg tcgaggcttc
     5041 cgttgtgttg tcctggtgtt ccgacgcaaa cgagcgagac atcggattcg aggacggccg
     5101 cgacggcgtc ggtggttgcg cgcagttttc ctgctttgac cgtttcggcg atgtactcct
     5161 cgatgcctgg ctccacgatc gggctttttc cgctcgagat gatgtctact ttgacctgat
     5221 tgacatccgc tccaatgact tcatgtccaa gcttcgcgaa gcacgcggct gaaacgcatc
     5281 ccacataacc gagtccgagc accgagattt tcatcttgtc tccttagcga tgatgcctac
     5341 gcgcacgctt gttgcgcgct cgctccatga tatcgacgag ggcgtcggtc gccctgtccc
     5401 agccaaaacg ttccaccacg gccttgcgag cagcgatctc gatcgcctca cgtgcctcgg
     5461 tgtcatgtat caaacggatg acttgttgcg cgaagtgcgc tggatcgtcc gcaagcacga
     5521 tttctttccc attttcgacc ggcaatccct cggctccgat ggtggtggat accaccgctc
     5581 gctgcatccc catcgcttca taaattttga tgcgcgttcc gccgccgacg cgaaggggaa
     5641 cgatatacac cgcggaccga tcgatgtagg gccggacatc gtcgaccctt cccgtcaagg
     5701 tgattttcgg ttctcgtttc gccaaggcct gtagcgttgg cgtgggattg cgccccacca
     5761 ccgtgacgcg aacttgaggt atttcagccg caatgcgcgg caaaatctca tcggcgaaga
     5821 aaaggatggc gtcctcgttg ggcatccaat ccatcgagcc agtaaacacg agatcgaaag
     5881 gctcccgggc aagcttgagg gcatggtctt gtggcagcgg actgaaaaaa tcgaaatcga
     5941 cccccgtcgg aaccgcgtac acatccttca ccccaaagcg ttgacgaatg gcctgggcat
     6001 ccggttccga gactgcggcc accgcatcgt atcgcttgca caacagatgc tcgtacgcgc
     6061 gcattttcac cgcttgtccg aagaaatagc ctttttttac tggatttttt tcattttgga
     6121 acgtgcgctc ccacaacacc gtttccacat tgtgttcgaa caacacggtc gcggcatgca
     6181 aacgacgcgg cacgttggcg ctcggtgtga gaaagtcaca gaccacgacg tcgaattctc
     6241 tccgagcatc ggaccgcgca aggaactggc gcatctgtgg ttgcttgtac ttttggatga
     6301 cgtagggaag gggagaggcg aggttttttg caagatcgac ataaaacccc gtagaaaaac
     6361 gcttgggctc gctccacgga accgtttgca gatgatgaca atactcactg gcacgctcac
     6421 gagcgtcggc cgcatccgtg ggactgctca aacaaagata cgtgacctcg tgcgaacgca
     6481 tcaaatgccg aagcatctcg aacgtccgga tctttccccc cttgtccacc ggatgtagca
     6541 gttcactttt gacccacagt actttcactg ctccctccaa aagaattcgc acggtatcac
     6601 agggatttgc caatcctcaa tcgcaaaccc ccatggcaaa cgaactgcct tgcaccatcg
     6661 cccgtcatcg caagtccgtc ttcttgatct tgcccgtcgt tgtcttgggc agatccggca
     6721 cgatctgcac atatttggga accatgaagg cttcgagtcg cgccttggct tccgtcaaaa
     6781 tgagcttttc cgtcaactcc gccccttgtt ccggcacgac gaacgctttg accgcttgtc
     6841 cgaaaatctc gtcgggtacg ccaatgacgg ccacctcttt gactccagga atatgaacca
     6901 acgcgttctc cacttccttg ggcgccacct tctccccccg agacttgatc acgtcgtcca
     6961 tgcgacccac gaagtacaaa tacccctcct catccatctt gcagtagtcg ccggtgtaca
     7021 acacgtattc gcccggcagc ggcccaggcc gaagcttctt tgccgtctgc tcgggcttct
     7081 cccaataccc ccgcatcacc gtcgcaccac gaatcacaag ctgtcccacc tcgttcggac
     7141 ccaccttgcg gtcttgctcg tcgacaatcc acatctccgt gttgggaatc gcgatcccca
     7201 cactcgtcat ctttcgacga atgtcctcgg gtggaagata cgtgcagcgc ttgcactccg
     7261 tcaggccgta catcgagaaa atacgggctt gcggaaatag atcgagcagc atctcgatgt
     7321 gtttgaccgg caacgcggct gccgtgttcg tgacgaatcg gatcgacgat aagtcgtaat
     7381 cacggaggtt tttgttttcc gaaaggatgg cgaaaatggt cggaactcct ggaaatcccg
     7441 tcactttttc ttctaccatc gtcttgagaa cttgcgcggg atacgtgaac gatcgttcga
     7501 ggaccaaccg cgcgcccaca cgaaacgaca tgagcatctg gtacaagcca tagtcgaacg
     7561 aaagcggtag cacgcaaacc acgacatcat cctcttgcat gcccagatac gtggacacgg
     7621 aaagaccagc ggtcagcatg ttgcgatgcg tgagcatcac gcctttcgga tctccggtgc
     7681 tgcccgaggt gtagatgatc gcggcaagat cgatgtcgat accggtgcgc gcgggaggca
     7741 cgcgcgggtt tcccgaagaa agggcttgat cgaacgtcat cacctgcaaa ttctcaggcg
     7801 tcggagaaag cggttttccc gtcatgatga ggactttgag gtgcttgttg tccgccacca
     7861 cgggagccaa gacgcgcgaa agcaaaccat cggcgatgag cgccgtcgcg cggcaatcct
     7921 cgagcaagta ccgaagcttg tcggtcttgg tgagcggatt gacgaccgac accaccgcat
     7981 tggctttgag caccgcccaa aacgcaatca ccgtctcgac actattgtct gcaaatacga
     8041 cgacacgatc gccacgggtg acgccttgct gcacgagggt gtgggccagc gcgttggatt
     8101 gggcatcgag ttcagcgtag gtatatcgtt ttttgtcgca gacgagggcg atcttgtcgc
     8161 cgagacgtgc tgccgaatgg atgaggtaat cttgaagctg aggaatggcg tgaagcatga
     8221 tggtttgctc ccttatttcg gggtgcagcc tacacggatc gatgctggcg atctatccgg
     8281 gaagcggtat cacactcgtg cgtgacccgc aggcagcctg tttgaccgct catgggaacg
     8341 aaaggccggg atgaaggggg gatgaaggta cgcgaggtgg agctgggagc ggtttttacg
     8401 acgatcgttc ggtcgtgcgg gggtggggaa tgggccacga acgcaacgag ccaagggtga
     8461 cttccaccag cgcgacgggg tgatccagcc cgaacgcaag aatgacttcc ggatgcaatt
     8521 cacccaagat gccttcgaaa gacccggctt gaaaacgcgc cgcacgccca gcggtaaacc
     8581 aaggttgctg cgtcgccgaa taggctgcct ccactcccat ttcacgaagc atcgcgtcca
     8641 aaatagagcg tgccgtcgca taccccgcgt ctttgcccat ctccacgaaa cacacacgac
     8701 gctcttcgcg gcaacccgtg ggcgcttcgt catcgagcaa ggccacgttg tcgatctcga
     8761 acagccgctg gggcatcggt cttcgacgat tgtcatgcag cgcttgcatg actccgctca
     8821 tcaaatgttg tcggacgacc gtcaaggctt tgagttttgg attgccgacg cgaggatacc
     8881 gattgggtac gggcatgcgg aaccgctcat aatgatccga ctcggtcgtc atgggcagac
     8941 tcatgatttc gctgtaccca aggccgagca agatggtgcg tgcaagatcg ctggtttgct
     9001 cctcggtgcg agaggaaccc acggtcatgg taggcacgag cttggcctgt atgttttgga
     9061 atccaaaccc gatggccacg tcttcgaaaa catcgaccgt atgacggatg tcgcttcgaa
     9121 aagcgggata tcgtacgcga aaccgggtct tggattcgtc gatgggctcg acatccaagc
     9181 gcatgcggaa aaggctttcc atcaacgtgt gggcatcgag cggcaagccc aaccattgct
     9241 tggcttgaga caggtcgacg atggcctgct tgggtgtaag gtctggagtt tcaagctctt
     9301 tttcatcttg gacgatacgc acggtgcgga tggtggcgcc caattccacc aaagaactga
     9361 cgagagtatg aagagtattt tcgacagccg cgatggatat gccggtgaca tcgacgaaca
     9421 accggtgtga gccgattttg attttcgtgc gttcgctatt gatgatggga ggcatggaaa
     9481 gcacgtgacc atcggcatcg acgagcacgg ggtaacggtc atggtttgcc aaaaggtggg
     9541 cgtaggcgat tcccttggaa tgctctgaca ggatttttcg tccactcatg ggttccgaca
     9601 cgccgagcgg cacgaacggt tcatcgtcgg gatgcaaggt tttgtacgtg atgggcccct
     9661 tgatcgtatc caggtcgtag atgccgatgg aagcgagttt gcggtccctt cccacacccc
     9721 aatgcaggtt ttcttgtagc ttcatgagcg cgacgagcat gcggtcatcg acagaaggca
     9781 gttcgatgac ggcacaacgt aggaacggac ggtagctatt gggttggcag acgctggggt
     9841 cgacgttcac gatgatgtcg gagcgggtgc tggtaaacga gggcaagcct tttttttctc
     9901 cgaggtatcc ttgcagggcg cgaacgagtc ctccgatatc gaataggtcg gggcgtgcgg
     9961 cgagcaggtc taaacgaatg gcggtgatgg tgtcgacgat ctcgaaaggc tgcatctgcg
    10021 tgaatccaca caggctgcat tgtttgaccg tgtcggcgcc cagggagcct tccaccaggg
    10081 cctggcattg ggggcagcgg tagcggggca gttcgacgac ttcctctacg tcgcaaccga
    10141 tttggtcgag ggcatcgatg agttgctgcg ggggataggg tttttcgagc aattcattga
    10201 gccaacttgc atcgatattg actatcggca tagggcaacc tcctccatgg catccaggct
    10261 cccctggtac aactggcgga tatccgaaaa accgaggcga agcatggcga ggcgttcgag
    10321 acccaagccc catgcgagca cggggtgctt acatccgaag ggttcgctga cctccggtcg
    10381 aaagatgccg ctgcctccca tttcgatcca cttgccacgg ctctccatat acaccatgac
    10441 atccacacta ggctccgtat acggatagaa cgcgggcttg aaccgcacct tggcaaagcc
    10501 cattttttgg tagaaagccg tcagggtgcc cagcagggtt gctaggctcg cttgctcgtc
    10561 gatcacgatg ccttcgattt gatgaaacac tggtaagtgc ttgaaagata tggtttcatt
    10621 tcgataggtc caacccacgc agaaggcctt gtacggcgga ttcggatgcg ctgcgagggc
    10681 acggatggtg gccgccgtcg tatgggttcg aagcacgact cgctcagctt ccctttcgct
    10741 ccatttatat ccccatccgg ttgatccggt atcgcctccg tgttcgtggg tgcgacgaac
    10801 cggttcgacg acctcggggg gaggcaaagg aatacgggcc ggatcgagca tatagaacgt
    10861 gtcttgcatg tctcgggcgg gatggtcttg cggttgaaaa agggcgtcga agttccaaaa
    10921 cgcgctctcg accatcggtg agaccacctc ctcgaacccg agttctagaa aagctctgcg
    10981 ggtctgttcc atgatttttc gaaggggatg gatcttggcc ggatacacgg tttttcccgg
    11041 taaggtgaca tcgtagggac gcagggtgat gtcgcgccag gcaccggatt ggaagtcttc
    11101 gggcgcgagc gcattctgct ctcgtttgac cttcgctcgt gagagcgcct cgagaccttg
    11161 cgtcgtcagg ctcaggatcc gttgggtgcg cttgcgcagt ttggcaaggt ccgtgcgctt
    11221 gccgagaagc tgttcgacag ccaccggatc gaccccctgc aacgcttgtt cgtcgaggaa
    11281 tcgagttcct tcgagggcca agcgaagcgc gcgttcgtcg tcagcaggct catcgacagc
    11341 caaccgcccc gcatcggtaa cgcggaccat gggcggctgg cctttcgttc gttcgaccca
    11401 tccgcgggca ctgccccagc ggaagatttc gtgaacgggc aggccttgtg cttgcgcgcg
    11461 ttgggtgaaa tcgggcacgg acaacgaacc cccggcgtcg atgagcaacc gcaaggcttg
    11521 gcgttccggc agcccttgtt gcaatcgtgc agcagcgtcc ggcaccggat ccagctcgag
    11581 acgcacccgc tccttcacat cgaaaaaacc ctgttgttgc gcctccagcg cggcaaccat
    11641 caccaggctt tgatccaatt gcgtggtttg caccacatca gccacgtctt tgggacccgc
    11701 ccgaacggct tcccagactt ttttagcggt ttcggtgaga acgacagggg caatcatcgt
    11761 acgataaact ccttggaggc ccgcagccgg gccgatatgc gcctaccagc aggggggaca
    11821 tagaatcaac catcggatgg atagaacctc cattctggcc attgcttgcc ggccaaggct
    11881 gcactacgaa agggtattcc catgacacgc attcgcgtcg tgggccgaga tcaaccgatc
    11941 gaagccccag aaggttccag tctcctcgcg gccttgttgg cagcgcgttc ccccattagc
    12001 acctcgtgcg gcggacaagc cacctgcggc ctgtgccgcc ttaccgtgct gcagggaagc
    12061 tcgttgcttt cccccgtcaa cgccaaggag gtcctgcacc tcggcaacgt ggcgaaagtc
    12121 gtcagcattc gcctggcatg ccaggctctc atcatgggcg aaggggaaat cgaaatcgat
    12181 gtgcccgccg ttgtcgatgt ggccgcaaga aaacgagaac aggcccgccg aggatttgcc
    12241 gagcgggcgc aacgtcgacg ctccgtgccg ggaacccagg cttccccgga gactcccttg
    12301 gagccggcgc ccaaagctgc cgtggaacga atcgaatggc gacctcgcat gctcaacccg
    12361 gggccggagc aaaccccgga ccctgaccta ccggccgacc gaaaacaaaa atcccagagg
    12421 tcttgaacga tgggggcgac aagggccctg gacgagccgg caagtaggtg ccagcgcccc
    12481 acgaggggga tgtggggaaa cactaggtat ttcaattagt tgcatcgtgt ttgacgcgga
    12541 atcatcgatc acgagccgag taattgcttt tccgaacgag cgtctgatga gcgtgagtta
    12601 atgtgttttt ccaattggtt atcgtttggc ggctcgttgg gggcgggggg tgatgcggcg
    12661 ttgggtctcg ttgggtcggc cggcgagcag agagtcatcg aactcgatcc cgggatgaca
    12721 tgaccttccg aatggtctag cgtgcgaccc ccaacaagac tcacaaggac gttggctgat
    12781 gcgcaaggac gaactcgaag ccgcctccac gctcggcagc ggcatttcta ttcagggccg
    12841 aatcatggga gagggaaacc tgaccgtgga aggctcggtc gaaggtgatg tgacgatcca
    12901 aggcgatttc gtcgtagccg cgggcgctcg cgttcacggt tcggtcgaag cgcatggtgt
    12961 cgtggtcgag ggcgatctcg aaggtgatat tcttgccacc ggcccggtcc atgcgggtgc
    13021 gggggcgagt ctgcgtggca atgtgaaagg tcaagctttc acgatggaag agggagccac
    13081 ggtttcggtg acggtcgatg cggatttcga tctgcctccg gaacttcaag ggggacgaga
    13141 gtagcaagta cgaacactcg tttcgtgcga gtggggagaa ctcctatggc aagtacggtc
    13201 attggtccag gaatcacgat cgaaggcgaa gtcatcgcgg acgatgacgt tgtcgttcaa
    13261 ggcaccattc gtgggaagct tacgtcccag gatgcggtca ttatcgaggc gggtgcgacg
    13321 gtggaagcgg acgtatcggc ggcatccgtg gtggtaagtg gaagtgtgac gggcgacgtg
    13381 caggctgctg atcgggtgga tttgcagtcg acgggtcgat tggtgggtga tgtcaaagcg
    13441 gcgcggctaa cgattgctga tggtgcgtcg ttcaagggca acgtcgacat ggacgtttga
    13501 ctttcgcacc gtgggcaagg tgtggagaag cgatctgcgg gaatccttcc tgcttgtgat
    13561 aggacgggtg aaaaaaaatg gccgacagcg aggcgatcat cgggcaaggg acgttcattc
    13621 gcggcaatct ccgtggtgag ggggatttat gtgtcgttgg gcatgtcgaa ggagtgattg
    13681 aggttcgtgg acaagtcatt ttcgaagaaa cctcgatcat tcaggcagat gtgaccgcgg
    13741 agcggatcat cgtgcgtggt gcgattgctg gtgagctttc ggcaaaagag tcgatcgtgc
    13801 tggaggaagg ctgccgggtg gtagggggat tgcgagcccc tcgacttcgt atcgagcctg
    13861 gagcgctgta tcggggacgc atcgacatga tggaaggggg tgcggttacg gcagcggcaa
    13921 gcgcgaagca acaatcgcgt cctgcggtac aggccacaca accgtcggcc gctggtcggt
    13981 cggcatcggt agtggcttct gcgcctgcgc gtccagctcc cgcgcgaccc gcgccttctc
    14041 gaacggcgcc gaaccgtcca ccggcgcgtc ccgctcgtgc gcaagcggta cagccgctgc
    14101 cggagcccga ggttcaaccg cagcccgcat catctgttgc ccttccgcgt gtggggcgca
    14161 tacgttccgc gcccgcggtt accgaatccg cgcccgtgca agccagtgat tctggggatg
    14221 tgccgccgcc gcccgtggtt ccagcgctga aaaagaaaac caaaggggcc gtgcgaaaac
    14281 gcggagatgc ttcatgaatg ccaagcgcga acgtttgatc gaactcctgc gggagctgtc
    14341 tttcgagcga aagcgcgtca ttttggcatc gggtcgtgag agcgactttt tcatcgattg
    14401 caaacagact gcccttaccg ccgaaggaca cgtgctactc ggcgaactga tgtttcacgc
    14461 tcttcggcaa ctcgctcccg tgaaagccgt cgctggggtg gaattgggcg gttgtccctt
    14521 ggcttccgcg gtggcgctga cgagcttttt gcacggggct cctttggtgg cgttgtacgt
    14581 tcgaaaaagc caaaaagacc acggaatgaa aaaaatgatc gaaggcgaca aatccatgca
    14641 cgaaggcatg gcggtcgccc ttgtcgaaga cgtgatcacc accggtggtt ccaccttgcg
    14701 tgcggctcag gtactgcggc aggcgggctt ggttccggtc ggggtggtgg cgattgtgga
    14761 tcggctcgag ggtggttcag acgccttgcg cgaagcgggt ttgcctttgg tggcgttgac
    14821 cacacgcaaa gacttcatcg cagattgaac cttcctgtcg tttcggggtg tgcgaacgcc
    14881 tgcggacttt cgggcatcgg tagggggcac tccgtgttgg agtctaggtc gtcgatcgtt
    14941 ccgtctttgc ttaggggggc caatcatggg ccggttggta tataacatgt tgaattgatt
    15001 gaattattga taatacccgc cggtgaaagg gggcgatgga gatcggtgat gggatgacgg
    15061 ggagggatga tgaggcaggt gtgttcggag attgggtagc cggttggggt tgtggagcca
    15121 acttcgcggc tgggtcgttt cagggcagcg acgatgaatt gtagccgccg atgcttgaca
    15181 tactgggacc catctcgcat ctgatgtcat actccggtgc agagacgttt tgtgatgagg
    15241 cgcgaattcg gcattccgtt cgtggcgtgg gtcggtgcgg ccgtcctggc gcatttgcta
    15301 tggacgacgg gagcggatcg ggttgcgcgg gtggtggagg atttcagtcg gattcgctgg
    15361 tttgtcgagt cggtgtccgc ggaagtggcg cgggcgcagc aggtggagat ctcgctcgag
    15421 gacatgcctt tgcaagcgga ggatgattcg gagcagagtc agcaagagcg agagagtcgg
    15481 gcaaaggaca agatggagca gccgccttcg ccggcggagt ccgagcccga gcctccggtg
    15541 gaatccaagc cgaagccaag cgatccgata caactatttc ccgtgccgtc ggtcgaagcg
    15601 cccaaggttc aggttgtgcc ggagatcaaa aaagatcagc gaattgcggt taagcagcac
    15661 gtcaaggaca aggatcagcc ggacaatccg aatgctcgtt tcattggtga tgaggccaat
    15721 catgtgcagg aggagacggt ggcgcgcatc acctctcacg atcaggacga tcagaatccg
    15781 acgcctggag gacagcattc gtcggaggat cgacgtccgg gcaatgcgga tgagacgaag
    15841 ataggtgata gtgaggatag ggagggggag ccggacaagg cgccgggcga gcatgtggtg
    15901 gaagagaagg aaggaaaagt ggatgagccg ccggcgcgga ctgcctcggc tccgtccgtg
    15961 gagcccaagg ccggagatcc tgcccgtgtt gcggcgcggg acaagccaat gacaccgcag
    16021 ccaccgccgc aacccacgac gatccccgaa gaggcagaac cgctggtgtc gggtcagggg
    16081 acttattccc tcgatcctcg tcgaaagaaa aagccggtgg atactcggag tccggggccg
    16141 tcgcgattga ccactcccaa ccgcgtggga ttgggagctg ggccgaatga gcgtggaatt
    16201 cagacgaatt tgacgatgga tgacgtggtg gcttccgtgg ggcgtgatga actggcgcgt
    16261 cagcgggcag ccgatggcga gcgtcgtcga agccagcatc gtggctcctg gcattcatcg
    16321 agtttcgagc ggtggcgcgg ggccattgag aattacgtat cgtcggttcg cccgggaaat
    16381 cagaccgcgc tcaacacggc gcgggttcct tttgccgcgt atctcgtgca aatccacaat
    16441 cggattcatc ctgtttttgc ggatactttt cttgcttccc tcgatgcgtt gcctttcgat
    16501 catccgatca acgactacaa gctcatggcg cggttggaga ttgtggtgga gcccaattcc
    16561 ggtcgggtga tcaagatggg ggtgatcaaa acgagtggaa tcactgcttt tgatgtgggt
    16621 gctttggatt ccgtgcagcg ggccgggcct tttggcaagg cgccttcggg catcatttcc
    16681 accgatggaa acgtctattt gcatt
//
LOCUS       DAOSOR010000045        16575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_3521_length_16575_cov_4.481477, whole genome shotgun sequence.
ACCESSION   DAOSOR010000045 DAOSOR010000000
VERSION     DAOSOR010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 16575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16575
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3521_length_16575_cov_4.481477"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="PK710_04075"
     CDS             complement(<1..1257)
                     /locus_tag="PK710_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HOT08921.1"
                     /translation="MSSRTDLQPAPRLVVAGASGDAGKTTVSLGLIRAFRSRGLVVQP
                     FKKGPDYIDPAWLTRAAGRVARNLDVRLFGENVVQAHFDRYAPQDGISVIEGNRGLYD
                     GGDAQGTHSTASLARLLSAPVVLVVDATKVTRTAAAVVLGCQRLEPQTPLAGVILNRV
                     AGSRHRRVVTEAIEQATGLRVYGALPRRRGDMIASRHLGLLTPEEHGAAEDALEVVRQ
                     WVEDHVDVAALVELAQGAPSRAMSLPLGQVEPAGQGVTIGVLRDASLSFYYPENIEAL
                     EALGARVVTISPLRDERLPSMDALYVGGGFPETHAKELSSNRALLGEIRQRIAGGLSV
                     YAECGGLIYMSSKLWVQGQAYDLVGALPLELQMEPRPVGHGYVDVQVSGDTTFYEKGQ
                     KLSGHEFHYSRIIGGLDRVERVFRVMR"
     gene            complement(1284..1535)
                     /locus_tag="PK710_04080"
     CDS             complement(1284..1535)
                     /locus_tag="PK710_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08922.1"
                     /translation="MAEPASVEEVAETMYKLVAQAQGQKKLKPGDLTKSAMQIFGDRV
                     DKKVCKEAIKNLIESGKCVYTYFGGSFIEIPHEEGAANK"
     gene            complement(1548..1889)
                     /locus_tag="PK710_04085"
     CDS             complement(1548..1889)
                     /locus_tag="PK710_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08923.1"
                     /translation="MPLNDPDTDGVSIWVGGKVSNARTEPRFSKLAIPFLPNNPPRWP
                     EVTEVVKKLVELWAADARKYERMGDWIERIGWPRFFKLAGIEFQKQHIDDFKHAGLTF
                     KRSTHIRFDKA"
     gene            complement(1932..2639)
                     /locus_tag="PK710_04090"
     CDS             complement(1932..2639)
                     /locus_tag="PK710_04090"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08924.1"
                     /translation="MAEAPAKRITDIGPPHHKKFLPPVILNNYGKWKYHEIPRPGVLL
                     HVGETGDKLWSVRAASPRLIAIDTLRWYADLADKYAGGHLRFTSRNNVEFLVTEEKNV
                     DPLIAELTAAGYPVGGTGNAISNIVHTQGWVHCHSAATDASGIVKCVMDELTPYFTSM
                     KLPGKLRIALACCLNMCGAVHCSDIAILGIHRHAPAIKHDQVKKVCEIPSVVAACPTA
                     AIRPATVEGNASVEVIE"
     gene            complement(2718..3908)
                     /gene="dsrA"
                     /locus_tag="PK710_04095"
     CDS             complement(2718..3908)
                     /gene="dsrA"
                     /locus_tag="PK710_04095"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - hydrogensulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     alpha"
                     /protein_id="HOT08925.1"
                     /translation="MAKVPTPKLDELEKGPWPSFVTEIKKRADKHAACSDLLGILERS
                     YNEKIGHWKHGGIVGVFGYGGGVIGRYCDLPEDFPGVAHFHTMRVNMPAAWFYTSEAI
                     RTLCDIWEKHGSGLTNMHGSTGDIIFLGCRTEDLEPCFAELAEAGFDLGGSGSDLRTP
                     SCCCGMARCEWACYDTMGFCYEMTQHFQDELHRPAFPYKFKIKAAGCPNDCVASIARA
                     DLSVIGNWRDNIQIDAAEVTNYAKAGLDIAADVVNNCPGQCIRWDGKTLTIKDEDCKH
                     CMHCINVMPKALRPGKERGATLLLGSKAPIMEGALLSSVLVPFIEMEEPYDDIKELIE
                     AIWEVWCEDGKNRERVGEFIQRVGMGNFLEAIGLEPDPNMVAHPRENPYVFYNLNEEE
                     ESDE"
     gene            complement(4210..6342)
                     /locus_tag="PK710_04100"
     CDS             complement(4210..6342)
                     /locus_tag="PK710_04100"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT08926.1"
                     /translation="MDKCGFFLCTGCGIDDVVDAKKLLGIAKKEGKAAFTVSHPKLCD
                     PQGLQVIRDAMEKEGLDAVVLGACSQREMTSVFKFDGTFVDRVALREFVAWSCGGDDD
                     EDKQMMAEDYVRMGCVRAQKAKKSEPVIMEEVTRAILVVGGGVAGMSAALEAAAAGYD
                     VTLVEKTDALGGLMAGWHKRIPSGGDWSKAQDNDVSTLIEAVKNEPRINVCTATEVAS
                     ISGAPGQYEVTLRGKSAAQVKIGAIVVAVGGKTYDADKLGELGFGKSANVITSAQLEQ
                     MAKEGKLVRPSDGKPIESVAFIQCAGSRDEKHLAYCSVYCCPTTLKQAGYLRQANPKT
                     RTYVLYKDMRCQGLMENYYRQTQSEGTIFIKTQIQQVDASNGMVKVRADDLLSGVELD
                     LEVDVVVLATGVEPLTKEAEILNLTYRLGKELPVRDYGFPNSHFICFPYETQRTGIYA
                     AGIVRQPMDSRAAINDGAGAALKAMQCVEAAAAGVAVHPRSMDITYPDFFLQRCTQCK
                     RCTEECPFGTLDEDEKGTPKPNPTRCRRCGICMGACPERIISFANYSVDMVGSMIKSI
                     EVPDEDEEKPRVLALVCENDALPGLDMAAQLRLKVSPWVRFVPLRCLGSMNLQWVNDA
                     LIKGFDGVMLIGCKFGDDYQCHFIKGSELCSVRLEKIQETLTRLMLEPERIKLVQFEM
                     GDAANLPGIINSFVEATEKLGPNPNKGF"
     gene            complement(6345..7607)
                     /locus_tag="PK710_04105"
     CDS             complement(6345..7607)
                     /locus_tag="PK710_04105"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT08927.1"
                     /translation="MSANNDKCVLVVGGGIGGMSAAIEAAEAGQHAVIVEATPSLGGR
                     VASMHRYFPKLCPPVCGLEINYRRIKSNPKIEVLTDATLTRLEGKAGDYQATVTIQAR
                     RVNDRCTACGDCIEPCPVERDDSYNYGIGKTKAIYLPMKLAYPMRFAIDPEACPGSSC
                     GKCLPACKFDAIELDMPTKERTFDVSCVVWATGWKPYDATRIENLGFGKYKNVVTNVM
                     FERLAALDGPNKGKLLRPSDGKEAKRVVFVQCAGSRDEDHLPYCSTVCCTASLKHACY
                     VREAYPDSKVEIFYIDLRVAGTAEDFLARVQADQGVSFNKGKVGQILENPDTGDLTVV
                     AEDIAGTSKTKRVEADLVVLATGLVPELASVQLPLAMTKDEHGFVVSNRPDGLMAVGC
                     ARAPSDVVRTVQDATAAAARAMQLGARR"
     gene            complement(7665..8486)
                     /locus_tag="PK710_04110"
     CDS             complement(7665..8486)
                     /locus_tag="PK710_04110"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOT08928.1"
                     /translation="MSNDDAQDMKEKILREYPRLGVNDKFKFACHSKLACFTHCCADV
                     NIALTPYDVLRLRKRIGMGSEDFIEKYTVLPPMAPGQKLPMVYLRMSEADKRCQFVGE
                     KGCSVYEDRPWACRMYPIGHASGRTEANPQGEDFYFILRDRRCDGLDEERELTIREWM
                     ESQATGDYDAANDEYKELSLHPFLTRGYALEPQHRQMFFMACYNLDRFREFIFESRFL
                     QKFIIEPETIEAIRHDDEALLRFAYRWLRFALFHERTITINPDYENAKKKVLRAQ"
     gene            complement(8483..8797)
                     /locus_tag="PK710_04115"
     CDS             complement(8483..8797)
                     /locus_tag="PK710_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08929.1"
                     /translation="MSIPTCPHCGAALKKWRTPPMSNWESPFIWVCFSDDCGYFQRSE
                     AWMQEKFNVRASYRHRLDPLTGETGPLPVWSRDALKDQIVEEEEDDSVSDAGLGDKES
                     SQ"
     gene            complement(8893..10779)
                     /gene="aprA"
                     /locus_tag="PK710_04120"
     CDS             complement(8893..10779)
                     /gene="aprA"
                     /locus_tag="PK710_04120"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009973 - adenylyl-sulfate reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA];
                     GO_process: GO:0019424 - sulfide oxidation, using siroheme
                     sulfite reductase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit alpha"
                     /protein_id="HOT08930.1"
                     /translation="MANFETITVETDLLIIGGGMGACGAAVEAAYWAKKNNVKVTVVD
                     KAAMDRSGAVAMGLSALNQYVGVGAGDNTVEDYVRYVRQDLMGVTREDLVFSIARHVD
                     STVHLFEKWGLPIWKDENDKYVHEGRWQVMINGESYKILVAEAAKNAIGVDNIIERVF
                     ITEPLMDGDRVAGAVGFSVRDEKFYVFKAKAVMVAAGGAVSVFRPRSVGEGLGRSWYP
                     PFNSGSSAYMTIKAGAEMTCQEVRFIPVRFKDGYGPVGAWFLLFKSAATNAFGENYMV
                     THAEELKKYAPYGLVKPIPACLRNHLGMMDIFAGKGPLYMQTAEAIQRIAAEAGDEKA
                     AKKKLKTLEAEAWEDFLDMTVSQAILWAAMNIEPEKKPSEIAPCEPYFIGSHSGASGA
                     WVSGPEDLQTSASKKDYFWGYTNMTTVKGMFAVGDASGASSHKFSSGTFTEGRIAAKA
                     AVKFCVENADRPNVSDATIEEFKARTLAPLDLYEANKNYANDDNINPNFIKPRDYMFR
                     LQKLMDEYAGGVSSQFTTNKHLLERGMELLAMLKEDSSKLAAGSLNELMRCWENIHRT
                     WQAEAHVRTLLFREETRWPGYYYRADTPDMDEKNWKCFANCKYDPKTGEWTMIKREIH
                     RIVE"
     gene            complement(10810..11253)
                     /gene="aprB"
                     /locus_tag="PK710_04125"
     CDS             complement(10810..11253)
                     /gene="aprB"
                     /locus_tag="PK710_04125"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009973 - adenylyl-sulfate reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA];
                     GO_process: GO:0019424 - sulfide oxidation, using siroheme
                     sulfite reductase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit beta"
                     /protein_id="HOT08931.1"
                     /translation="MPSFVINDKCDGCKGKDKTACMYICPNDLMVLDKEKMKAFNRAP
                     DMCWECYSCVKICPQQAIDVRGYADFVPLGASCVPLRGSEDIMWTIKFRNGSVKRFKF
                     PIRTTSEGSAVPNGGFDEGNGDLNDFALFTEPASLHMDAVPTLKK"
     gene            complement(11743..11898)
                     /locus_tag="PK710_04130"
     CDS             complement(11743..11898)
                     /locus_tag="PK710_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08932.1"
                     /translation="MSEVAEEGCLAATYDVTGGFARMNVVEGDDGNTLLGDAVGSGVG
                     NSVGISC"
     gene            12007..12174
                     /locus_tag="PK710_04135"
     CDS             12007..12174
                     /locus_tag="PK710_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08933.1"
                     /translation="MKRALLFMGTTSPFAPAPRASAPLGRFPMLPHRATCAPVAVVRA
                     ASPSTGHLPEE"
     gene            12180..13454
                     /locus_tag="PK710_04140"
     CDS             12180..13454
                     /locus_tag="PK710_04140"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOT08934.1"
                     /translation="MSEKRPQPIVQGSVLVVGGGITGMSVAIEAAEAGLEAHIIESSP
                     TLGGRVSQLTKYFPKLCPPICGLEINYRRLRVQERVHVHTLSRVTKLQGSPGDFQVTV
                     EHRPRFVNDRCTACNACVDPCPVRRNNDHDFGMSSTKAIYLPHAMAYPQRFVIDEQAC
                     QGVSCSKCVEACSFGAIDLSQKTTTETLRVGAVVVATGWQPYDATRLTNLSFGKHDDI
                     ITNMMMERYANPQGPTKGKIVCPSDGREPETVVFVQCAGSRDENHLPYCSGICCMASL
                     KQATYVRAALPNAKIRIFAIDIRTPGRLEDFYTKVREDPNVSIERGKVARIDEIDGQL
                     RLQVEDTLGDKIQDVYADLVVLATGMEPSIRRDPLPFDIRQDEDHFGITDVATGLIVA
                     GCAKRPFGVSDSVQDATGAALRAIQCQAGRSE"
     gene            13454..15595
                     /locus_tag="PK710_04145"
     CDS             13454..15595
                     /locus_tag="PK710_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT08935.1"
                     /translation="MDKKIRVYFCTGCGIGDAVDVADLQKTAKKTSKNAAETRSHEML
                     CSPEGRAMIESDIANDGVNAVLVAACSPRVHQNTFHFGPSTIVDRIALREHVAYVLPA
                     GEEDTQMAADDYVRMGVTKIMKTEPMDPAVTDVQASILVVGGGVTGLSAALETARAGV
                     RVVLVEKQEKLGGWVASWGRKFPTRPPYRDLVDNDIVNTIEAVEKHPLITVYKAASVL
                     SISGQPGEFDVRIRVGNDEKKETIGAIVQATGWRPYDATKLTHLGYGKSKDVITHVEL
                     EEMLSKTGKLVRPSNGAVPRSVVFVQCAGSRDPNHLPYCSAVCCRTSLKQARVIRELY
                     PDTGVTLLYKDVRTPGQYELFYKNAQDDPGVFLVKGEVTGVAVDQKTMTVSMTDSLLG
                     DAVEVGADLVVLAVGMTPVSEEPILNLQYRRGGELPVGKYAFPDSDFICFPYETQRTG
                     IYAAGCVREPMDSDACSEDAAGASLKAIQCIACTARGAAVHPRAGDLTYPEFFLSRCT
                     QCKRCTEECPFGTLDEDEKGTPKPNPGRCRRCGVCMGACPERIISFKNYSVDMIGSMI
                     KAIEVPEEDEEKPRYVAFVCENDIIPALDLSSSLRKKISPWVRFVSLRCLGSTNLQWI
                     NDSLIKGIDGVMLIGCKSGEDYQCHFIKGSELCGVRMSKIQETLTRLALEPERIAVKE
                     FSMGDWESLPDWINEFVDKTNELGPNPNKGF"
     gene            15653..>16575
                     /gene="qmoC"
                     /locus_tag="PK710_04150"
     CDS             15653..>16575
                     /gene="qmoC"
                     /locus_tag="PK710_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-interacting membrane-bound
                     oxidoreductase complex subunit QmoC"
                     /protein_id="HOT08936.1"
                     /translation="MSKIERVDPDLKFVRKIVENGGGSVKRCYQCATCSVVCTLSPKD
                     KPFPRKEMLWAQWGLKDRLVGDPDIWLCHHCGDCTVHCPRGAKPGETLQAIRASVISE
                     LSFPKAIAEAVNKPQYFPALLLLPMLLLGVTMWEAGTLKAHPGPIIWGNLLPHSVFFE
                     GMFMLLMGWSVVAGGVGIVRQWQHMKRMCPADKDAKGLPIGAAFVQAVKELLWHSRFE
                     ACGQNRARTWPHRLLFFGFLSAMITAGGAAILEKAFHIPPPLPNPDAAAWAYAVGMFF
                     KILGILGAVGLLVGSGWLLNRRMVNQDQTGDS"
BASE COUNT         3562 a         5065 c         4370 g         3578 t
ORIGIN      
        1 acgcatcacc cgaaatactc gctccactcg atccaatcca ccaatgattc tcgaataatg
       61 aaactcatgc ccgctcagtt tttgcccttt ttcataaaac gttgtgtctc cactcacctg
      121 cacgtccaca taaccatgcc ccaccggacg cggctccatc tgcagttcca acggaagcgc
      181 tcccaccagg tcataggctt gcccctgcac ccataatttg ctcgacatat agatcaatcc
      241 cccacactcc gcatacacac tgagcccccc agcaatccgc tgccggatct cccccaataa
      301 cgctctattc gacgataatt ccttcgcatg ggtctccgga aatcccccgc ccacgtatag
      361 cgcatccatc gacggcaaac gctcgtcgcg caggggcgaa atcgtcacca cccgcgctcc
      421 caatgcctcc aaagcttcaa tattttccgg ataatagaag gataaggagg catcccgaag
      481 cactccaatc gttacccctt gccctgccgg ctccacctga cccaacggca agctcatcgc
      541 ccgcgaaggc gccccctgcg ccaactccac cagcgcggcc acatctacat gatcctcaac
      601 ccactgccgc accacctcca aggcatcctc cgccgcgcca tgctcctcag gtgtcaacaa
      661 ccccaaatgc cggctcgcga tcatatcccc acgcctgcga ggtagcgccc catatacccg
      721 cagccccgtg gcctgctcga tcgcctccgt aaccacacga cgatgcctgc tgcccgccac
      781 ccggttcaaa atgacccccg ccaaaggcgt ttgtggctcg agcctctggc acccaagaac
      841 caccgccgcc gccgtcctcg tcactttcgt cgcatccacc accaacacca ccggcgccga
      901 caaaagccgc gccaacgatg ccgtactatg cgtgccttgg gcatcccccc catcgtatag
      961 cccacggttt ccttcaatga ccgagatgcc gtcctgtggc gcatagcggt cgaaatgggc
     1021 ctgcacaacg ttttcgccaa acaaacgcac atcgagattg cgggcaaccc gcccggccgc
     1081 ccgggtcaac cacgcggggt caatataatc cggacctttt ttgaaaggct gtacgaccag
     1141 cccccgcgac cgaaaagccc ggatcaaacc aaggctaacc gtggttttcc cagcatcccc
     1201 cgacgctcca gccaccacaa ggcgcggtgc tggctggagg tcggttctgg aggacatcgg
     1261 tgaaatggat aagacagatg ggtctacttg ttggcagccc cttcttcgtg agggatttcg
     1321 atgaagctac caccgaagta cgtatacacg catttgccgg actcgatgag gtttttgata
     1381 gcctctttgc agaccttttt gtcgactcga tcgccaaaga tctgcatcgc ggacttggtc
     1441 aaatcgcccg gcttgagttt tttctggccc tgcgcctgag ccacgagttt atacatggtc
     1501 tcggctactt cttcgacact cgcgggttct gccatgatcg ctctcctcta cgccttgtcg
     1561 aagcgaatat gcgtcgaccg cttgaaggtc aagccggcgt gtttgaagtc atcaatatgc
     1621 tgtttctgga actcgattcc cgcaagcttg aagaaccggg gccagccaat tcgctcgatc
     1681 cagtcgccca tacgctcgta tttgcgcgca tcagccgccc aaagctccac cagcttcttg
     1741 accacctcgg tgacttccgg ccaccgcggc ggattgttgg gcaaaaatgg gatggcgagc
     1801 ttcgaaaacc gcggttccgt ccgcgcattc gataccttgc cgcccaccca aatggagaca
     1861 ccgtccgtgt cagggtcgtt caacggcatg gccgggcaaa ccgtgtagca gtttgcacaa
     1921 tacatgcagt tttactcgat aacctccacc gaagcgttgc cttccaccgt cgccggacga
     1981 atcgccgccg tggggcaggc cgccacaacc gatggaattt cgcaaacttt tttgacctga
     2041 tcgtgcttga tggccggggc atgccggtga atgccaagaa tcgcgatgtc cgaacaatgg
     2101 accgcaccgc acatattcag gcagcatgcc agagcaatgc gaagtttccc cggcaacttc
     2161 atgctcgtaa agtacggcgt caattcgtcc atcacgcact tgacaatgcc cgaggcgtcg
     2221 gttgccgccg agtggcagtg aacccacccc tgggtatgca cgatattcga aatggcgttg
     2281 cccgtacctc cgactggata accagcggcc gttagctcag cgatgagcgg gtctacgttt
     2341 ttctcttccg tgacgaggaa ctcgacgttg ttgcggctgg tgaagcgcag atggccgccg
     2401 gcgtatttgt cggccaagtc agcataccaa cgaagcgtgt cgatcgcgat gaggcgcgga
     2461 gacgccgcac gcacgctcca caacttgtcg cccgtctccc ccacgtgcag caacacgccg
     2521 gggcgaggga tctcgtgata cttccacttg ccgtagttgt tcagaataac cggcggcagg
     2581 aatttcttgt gatggggcgg tccgatatcg gtaattcgct tggcaggtgc ttcagccatg
     2641 gttttttctc ctcagaacgg aagccttgct tccgtgaatg atcactattc ccaaaaacct
     2701 acgagtgaaa ggacggctta ctcgtcactc tcctcttctt cgttgaggtt gtagaacacg
     2761 tacgggttct cccggggatg ggcgaccatg ttggggtcag gctcgagccc aatcgcctcg
     2821 aggaagttgc ccatgcccac gcgctggatg aactcaccca cgcgctcacg gttcttgccg
     2881 tcctcgcacc atacctccca aatcgcttcg atcaattcct tgatgtcgtc ataaggctct
     2941 tccatctcga tgaaaggaac gagcaccgag gacaaaagcg cgccttccat gatcggcgcc
     3001 ttggaaccaa ggagaagcgt tgcaccccgt tccttgcccg gacgaagcgc cttgggcatc
     3061 acgttgatgc aatgcataca gtgcttgcag tcttcatcct tgatggtcag cgtcttgccg
     3121 tcccaacgaa tgcactgtcc cggacagttg ttgacgacat cggcagcgat atcgagaccg
     3181 gccttggcgt aattggtcac ctccgccgcg tcgatctgaa tgttgtcgcg ccagttgccg
     3241 atcaccgaca agtccgcgcg ggcaatcgaa gccacacaat cattggggca tcccgccgcc
     3301 ttgatcttga acttgtaggg gaaagccgga cgatgcaatt cgtcctggaa atgctgggtc
     3361 atctcgtaac agaagcccat cgtgtcgtaa caagcccact cgcaacgggc catcccgcag
     3421 cagcacgagg gcgtacgaag atcggagcca gaaccgccga gatcgaatcc ggcttccgcc
     3481 agctccgcaa aacacggttc caaatcctcc gtacgacaac cgaggaagat gatgtcaccg
     3541 gtagacccat gcatattggt cagacccgaa ccatgctttt cccaaatatc gcagagcgta
     3601 cgaatcgcct cggacgtgta gaaccaggcc gccggcatat tcacacgcat cgtatgaaag
     3661 tgcgccacgc ccgggaagtc ctcgggcaaa tcacagtatc gcccgattac gccgccgcca
     3721 tagccaaaca cgccgacgat gccgccatgc ttccaatgcc ctattttttc attgtaggag
     3781 cgttcgagaa tgccgagcag gtccgaacaa gccgcatgct tatccgcgcg cttcttgatt
     3841 tctgtgacga agctaggcca cggacccttc tcgagttcat ccagcttcgg tgtgggtact
     3901 ttcgccatcg aattactcct ccttgactcc aggattcatg ccgcccaccg gacgcatcac
     3961 ttcactcaca cgtaccaggg acgactgcct tccggtcgcg tggcggcatg tccttatacc
     4021 gcaacatgcc gtctttcgcg aaccgatcat tcacaattta cactactatc tatccactcg
     4081 tgaaaacagc tcaaaatatc tctcttgccg gtcgttcgac ggaaaacgat aaccgtgttt
     4141 tttcgtggtg ccgtgcggtc gtaatctaat attcctatta tattacaaca ggttatcgcc
     4201 aaaccgctct cagaaccctt tgttcggatt cggtcccaac ttctccgtgg cttccacaaa
     4261 ggaattaatg atgcccggca ggttcgccgc gtcccccatc tcgaattgaa ccagcttgat
     4321 acgctccggc tccaacatca gacgcgtcaa cgtttcctgt atcttctcca gccgcacact
     4381 gcacaactcc gaccccttga taaagtgaca ttggtagtcg tcgccgaact tgcatccaat
     4441 caacatcacc ccatcaaaac ccttgatgag cgcatcgttg acccattgca agttcatcga
     4501 acccagacac cgcaaaggta cgaatcgcac ccaaggactc actttcaatc gaagctgcgc
     4561 cgccatgtcg agacccggca acgcatcgtt ttcacaaaca agcgccagca cccgcggctt
     4621 ctcttcatcc tcgtccggta cctcgatcga cttgatcatc gaaccaacca tatctaccga
     4681 ataattcgca aacgatatga tgcgttccgg acaagctccc atgcaaatgc cgcagcgacg
     4741 acatcgcgtg ggattgggtt tgggcgtccc tttctcatct tcatcgagcg taccgaaagg
     4801 acactcctcc gtacaacgct tgcattgcgt acaacgctgc aagaagaaat ccggatacgt
     4861 gatgtccatc gaacgtggat gaaccgccac ccccgctgca gccgcttcca cgcattgcat
     4921 cgctttcaac gccgctccag caccgtcgtt gatcgccgcc cgcgagtcca tcggctgacg
     4981 cacgatccct gccgcataga tccccgttcg ctgtgtttcg tacgggaaac aaatgaagtg
     5041 cgaattgggg aatccataat cccgtaccgg tagctccttg cccaatcgat acgtcagatt
     5101 gagaatctcc gcttccttcg tcaaaggctc gacacctgtc gcaagaacca ccacatcgac
     5161 ttcgagatcc aactccacgc cggacagcaa atcatccgcg cgcaccttca ccattccatt
     5221 cgacgcatcc acttgttgga tttgcgtctt gatgaagatc gttccttcgc tttgcgtctg
     5281 gcgatagtag ttctccatca acccttgaca gcgcatgtct ttgtacaaga cataggttcg
     5341 cgttttggga ttggcttgcc gcaagtagcc ggcctgcttg agggtcgtgg gacagcagta
     5401 caccgagcaa tacgcgagat gtttctcatc ccgcgagccc gcgcattgaa tgaacgccac
     5461 cgattcgatg ggcttgccgt cggaaggacg aaccagcttt ccttccttcg ccatttgctc
     5521 gagttgcgca ctcgtgatca cgttggcgct ctttccaaac ccaagctcgc caagcttgtc
     5581 cgcatcgtag gtcttgccac ccaccgcaac gacgatcgct ccaatcttga cttgtgccgc
     5641 gctctttcct cgcagcgtca cttcgtattg acccggagcg ccagagatgg aagccacctc
     5701 ggtggccgtg catacgttga tccgaggttc gtttttcacg gcctcgatca acgtcgatac
     5761 gtcattgtct tgcgccttgg accaatcacc cccgctggga attcgcttgt gccaccccgc
     5821 catcaatcca ccgagggcat ctgtcttttc cacaagcgtc acatcataac cagccgcggc
     5881 cgcttcgagc gccgcactca tacccgccac accaccgccc accaccaaaa tggcgcgcgt
     5941 cacctcctcc atgatgaccg gctcgctctt cttggccttc tgcgcgcgaa cacaccccat
     6001 ccgcacataa tcctcagcca tcatctgctt gtcttcgtca tcgtcgcctc cgcaactcca
     6061 ggccacgaat tcacgaaggg ccacccgatc gacgaaggtg ccatcgaatt tgaatacgga
     6121 ggtcatctca cgctgcgaac acgcgccaag cacgacggcg tcgagaccct ctttttccat
     6181 ggcgtcccga atgacttgaa gtccttgcgg atcgcaaagc ttcggatgag aaacggtaaa
     6241 cgccgccttc ccctcttttt tcgcaatgcc cagcagtttc ttggcgtcga ccacgtcgtc
     6301 gatgccgcaa ccggtacaca agaagaatcc gcacttgtcc atggctacct cctcgctccg
     6361 agctgcatgg cgcgagcggc cgcagccgtg gcatcttgaa ccgttctcac gacatccgat
     6421 ggcgcacgcg cgcatccaac cgccatcaag ccatccggac gattcgatac gacaaagcca
     6481 tgctcgtctt tcgtcatggc cagcggaagc tgcaccgatg ccagttccgg aacgagtcca
     6541 gtggcaagca cgacaagatc cgcttccaca cgcttggtct tgctcgttcc cgcgatgtct
     6601 tccgcaacca ccgtcagatc gccggtatca ggattttcga ggatctgccc cactttccct
     6661 ttgttgaacg acacaccttg atcggcctgc acgcgagcca aaaaatcctc tgccgtgccc
     6721 gccacccgca agtcgatata aaaaatctcg accttcgaat ccggataggc ctcccgcaca
     6781 tagcacgcat gcttcagaga agccgtgcag catacggtgg aacaataggg caagtgatcc
     6841 tcatcgcgag aacccgcgca ttgtacgaac acgacacgct tggcttcctt gccatccgaa
     6901 gggcgaagca gctttccctt gttggggcca tcaagcgcgg caagacgctc gaacatgacg
     6961 ttggtcacga catttttgta tttgccgaat ccaaggtttt cgattcgtgt cgcgtcgtag
     7021 ggcttccaac cggttgccca aaccacgcaa gaaacgtcaa aggttcgctc cttcgtgggc
     7081 atgtcgagtt cgatcgcgtc gaacttacac gcgggcaaac acttgccaca ggaactgccg
     7141 ggacacgctt cgggatcgat cgcgaagcgc atcggatagg caagcttcat cggaagatag
     7201 atggccttgg tctttccgat tccataattg tacgagtcat cacgttcgac agggcacggt
     7261 tcgatgcaat cgccgcaggc cgtgcatcgg tcgttgaccc ttcgcgcttg aatagtgacc
     7321 gtggcttgat aatccccggc tttgccttcc agacgagtga gggtggcgtc ggtgagcact
     7381 tcgattttcg gattgctctt gatgcggcgg tagttgatct ccaaaccgca aacaggcgga
     7441 cacagctttg ggaaataccg atgcatgctg gcgacacgtc cgcccagtga aggggttgcc
     7501 tccacgatga cggcgtgctg tcctgcttcc gcggcctcga tggcagcact cattccgccg
     7561 atgccgccac cgaccaccag gacgcacttg tcattgtttg cgctcatggg agtccttcct
     7621 tgtggttcgt tgaaaacgtc taaccttccg ctccagcaac cggatcactg cgctcgcaag
     7681 acctttttct tcgcgttttc gtaatccgga ttgatggtga tcgttcgttc gtgaaacaaa
     7741 gcaaagcgaa gccaacgata ggcaaagcgc agcaatgcct cgtcgtcatg gcgaatcgcc
     7801 tcgatcgtct cgggctcaat gatgaatttc tgcaagaatc tgctttcgaa aatgaactcg
     7861 cggaacctgt cgaggttgta gcaagccatg aaaaacatct ggcgatgctg cggctcgagg
     7921 gcataccccc ttgtcaaaaa cggatgcaag gaaagctcct tgtattcatc gttcgccgcg
     7981 tcatagtccc cggtggcttg actctccatc cattcgcgaa tggtcaactc acgctcctcg
     8041 tccagaccat cgcatcgccg atcgcgaaga atgaagtaaa agtcctctcc ttgcggattg
     8101 gcttccgtac ggcccgaagc atgaccgatc ggatacatcc gacaggccca cgggcgatcc
     8161 tcgtacaccg agcacccctt ctcaccgacg aactgacaac gtttgtcggc ttcgctcatg
     8221 cgcaaataca ccatgggcag tttctgacca ggagccatgg gaggaagcac ggtatatttt
     8281 tcgatgaagt cttcgctgcc cataccaatt cgcttgcgaa ggcgaagcac gtcatacggc
     8341 gtgagcgcga tattgacgtc ggcgcagcaa tgcgtgaagc acgcgagttt tgaatgacaa
     8401 gcgaatttga atttgtcgtt caccccgagg cgaggatact cgcggaggat cttttctttc
     8461 atgtcctggg cgtcatcgtt actcactggg aactctcctt atcgccgagt cctgcatcgg
     8521 aaacagagtc gtcttcttcc tcttccacaa tctgatcttt gagagcgtcg cgagaccaca
     8581 cgggaagcgg ccccgtttca cccgtcaacg gatcgagtcg atgccgatag ctggcgcgaa
     8641 cgttgaattt ttcttgcatc cacgcttcgg agcgctggaa gtatccgcaa tcatcgctga
     8701 aacaaaccca gatgaagggg gactcccaat tggacatcgg aggggtccgc cattttttca
     8761 aggcggcacc acagtgagga caggttggta tgctcatgat tcgcttgtgg gtgttggtcc
     8821 taagggcatg aaaaagaaaa atcctccagg ccaactgctc ctggaggatt tctcgttcac
     8881 atcacgcctc gactattcga cgatacgatg gatctcacgc ttgatcatcg tccattcgcc
     8941 ggtcttcggg tcgtacttgc agttcgcaaa gcacttccag tttttctcgt ccatgtcggg
     9001 cgtatcggcg cggtagtagt atcccggcca acgcgtctct tcacggaaca acagggtgcg
     9061 gacgtgcgct tcggcttgcc atgtgcggtg aatgttctcc cagcagcgca tcagctcgtt
     9121 gaggctccca gcggcgagct tggaggagtc ttccttcagc atggccagaa gttccatacc
     9181 acgctcgagc aggtgcttgt tggtggtgaa ttgcgaggaa acaccaccgg cgtactcgtc
     9241 catgagcttc tgcagacgga acatatagtc gcgcggcttg atgaagttcg ggttgatatt
     9301 gtcgtcgttc gcgtagttct tgttggcctc gtataggtcg aggggcgcga gcgtacgggc
     9361 tttgaactcc tcgatggtgg catcgctgac gttcggccgg tcggcatttt cgacgcagaa
     9421 cttgacggcg gccttggcgg cgatacgacc ctcggtgaag gtgcccgagg agaatttgtg
     9481 ggaggaagcg ccggaagcgt cgcccaccgc gaacatgccc ttgacggtgg tcatgttcgt
     9541 gtagccccag aagtaatcct tcttggaggc gctggtctgc agatcctcgg gacccgaaac
     9601 ccacgcaccg gaagcgccgg agtgcgatcc gatgaagtac ggctcgcagg gcgcgatctc
     9661 ggagggcttc ttctcgggct cgatattcat ggccgcccac agaatggcct gggatacggt
     9721 catgtcgagg aagtcttccc acgcttccgc ttccaacgtc ttcagcttct tcttggcggc
     9781 tttctcgtcg cctgcttcgg cggcgattcg ctggatggcc tcggcagtct gcatgtacag
     9841 cgggcccttg ccagcgaaga tgtccatcat gcccaagtgg ttgcgcaggc aagccgggat
     9901 gggcttgacc aagccgtagg gcgcgtactt cttgagttct tcggcatgcg ttaccatata
     9961 gttctcaccg aaggcattgg tggccgccga cttgaacaac aggaaccagg ccccgaccgg
    10021 accatagcca tccttgaagc gcacggggat gaaacgcact tcctgacagg tcatttcggc
    10081 accggccttg atggtcatat acgccgaaga accgctgttg aacggcgggt accaggagcg
    10141 tcccaagccc tcacccaccg agcgcgggcg gaacacgctc acggcaccac cggcggcaac
    10201 catcaccgcc ttggccttga agacgtagaa cttctcgtcg cggacggaga agcccacggc
    10261 gccagcaacg cgatcaccgt ccatgagagg ctcggtaatg aaaacgcgtt cgatgatatt
    10321 gtcgacgccg atggcgttct tggcagcttc tgcgacgagg attttgtacg actcgccgtt
    10381 gatcatgacc tgccagcggc cttcgtggac gtacttgtcg ttttcatctt tccagatcgg
    10441 caagccccac ttttcgaaga gatggacggt ggagtcgacg tggcgggcaa tggagaagac
    10501 gaggtcttcg cgcgtgacgc ccatgaggtc ttgtcggacg tagcggacgt aatcctcgac
    10561 cgtgttgtcg ccggcaccaa cgccgacgta ttggttgaga gcggacagac ccatcgcgac
    10621 ggcgccggag cggtccatgg cggctttgtc gacgacggtg actttgacgt tgttcttttt
    10681 tgcccagtaa gcggcttcga cggcagcgcc gcaggcgccc atgccgccac cgatgatgag
    10741 taggtcggtt tcgacagtaa tggtctcgaa gttagccatg ttgctttctc ccgatattcc
    10801 cgaagtcaat cactttttga gtgtaggaac cgcgtccata tgaagggaag ccggctcggt
    10861 gaagagggcg aagtcattga ggtcgccgtt accctcatcg aaaccgccgt tgggcacggc
    10921 agagccttcc gaagtggtgc ggatcgggaa cttgaagcgc ttgacgcttc cattgcggaa
    10981 cttgatggtc cacatgatgt cctcggaacc gcgaagaggt acgcaggagg cgccgagggg
    11041 gacgaagtcc gcatagccac ggacgtcgat ggcttgctgt gggcagattt taacgcagga
    11101 atagcattcc cagcacatat ccggtgcgcg gttgaatgcc ttcatctttt ctttgtcgag
    11161 gaccatgagg tcgttcgggc agatgtacat gcaggcagtc ttgtccttgc ccttgcagcc
    11221 atcgcactta tcgttgatga cgaagctcgg catattgcgt ctccttctct aacgttccca
    11281 ggtcagaggt tcatacccga ccggcacctg aaccgtcggg atcacttccc actatccaca
    11341 ttgggtggcg gcaaccgttt gccattcacc cgaaaaaaca agctagcccg tgctgcaaca
    11401 gagaaaaaag aaacccaaac caaaacatag gctcaatgcc ctgtacccca agcttccact
    11461 acgcttccga ggtactacac acggagacca tcatgggcct ctcgggatcc gacaggttgg
    11521 cattccttcc tgtctctgcc ctaatgcgtc gtcccaccga gacgatgcat gcgggttgtg
    11581 cgctaatcca acgggggcgt caagacaatt tcgtagccga gaacaaggat tttgcgggag
    11641 gattttatcg gccaagctct tgtcgttagc tgtagctaac gagataaacc ttacacagta
    11701 tacaaggcag taccttccgc aacgacagta tttatctacc ccttagcagc taatcccaac
    11761 gctatttcct acccctgagc caacggcatc ccccagcaag gtgttcccat catctccctc
    11821 cactacattc attcgagcga aacctccggt tacatcatac gttgcggcaa gacatccttc
    11881 ttcggcaacc tcgctcatcg acggctccgt tgtctcttta ttcctctccc gcactttacc
    11941 cacccccgac ttcgatctac atcatctcat gcctgccgtt cttgccagtc ggttcaggca
    12001 tcatgcatga aacgcgcgct cctatttatg ggaaccacgt cgccttttgc gcccgcgcct
    12061 cgagcctccg cgcctcttgg ccggttcccg atgctgccac atcgcgccac ctgcgcaccc
    12121 gtggctgtcg ttcgagccgc aagcccttcg acggggcatc tccccgagga gtaaggagta
    12181 tgagcgaaaa acgaccccaa cccatcgtcc aaggaagcgt cctcgtcgtg ggaggcggca
    12241 tcacaggcat gtcggtcgcc atcgaggctg ccgaggcggg actcgaagcc catatcatcg
    12301 agtcctcgcc aaccctcggc ggtcgcgtct cccaactcac caaatacttt cccaaactat
    12361 gccctcctat ctgcggcctc gaaatcaact accgacggtt gcgtgtccaa gagcgtgttc
    12421 acgttcatac cctcagccgc gtcaccaaac tgcaaggttc ccccggagac ttccaagtca
    12481 ccgtcgagca tcggccacgc ttcgtcaacg accgctgcac cgcatgtaac gcgtgtgtcg
    12541 acccgtgtcc ggtgcgacga aacaacgacc acgatttcgg catgtcgtcg accaaggcga
    12601 tctacctgcc gcacgccatg gcgtacccgc aacgtttcgt catcgacgaa caagcgtgcc
    12661 aaggggtgtc ttgttcgaaa tgcgtcgagg catgctcgtt cggggcgatc gacttatccc
    12721 aaaagacaac gacggaaacg ttgcgtgttg gggcggtggt cgtcgccacc ggctggcaac
    12781 cgtacgacgc cacacggttg acgaatctca gttttggcaa gcatgacgac atcatcacca
    12841 acatgatgat ggagcggtat gcgaaccctc agggccccac caagggaaag atcgtctgtc
    12901 cctcggatgg tcgcgagcca gaaactgtcg tgttcgtgca atgcgccggc tcacgcgacg
    12961 aaaaccacct tccctattgc tcgggcatct gctgcatggc ttccctcaaa caggccacct
    13021 acgtgcgcgc ggcgctgccc aacgccaaaa ttcgtatctt cgctatcgat attcgaacgc
    13081 ccggacgtct cgaggacttt tacaccaagg tgcgagagga tccgaacgta tccatcgaaa
    13141 ggggcaaagt cgcccgaatc gatgagatcg acgggcagct tcgtcttcag gtcgaagaca
    13201 ccctcggaga caaaatccaa gatgtctacg ctgacctcgt cgtgcttgcc acgggcatgg
    13261 aaccgtcgat ccgacgcgat ccgctgccgt tcgacattcg tcaagacgag gatcactttg
    13321 ggataaccga tgtggccacg ggcttgattg tggccggatg cgcgaagcgt ccgtttgggg
    13381 tgtcggatag cgttcaagac gcaacgggag ctgctcttcg ggcaatccaa tgccaggcag
    13441 ggaggagcga gtgatggaca agaagattcg cgtatatttc tgtacgggct gcggcatcgg
    13501 tgacgccgtg gacgtcgccg accttcagaa aaccgccaaa aaaacctcga aaaatgccgc
    13561 agagacgcgc tcccacgaaa tgctttgcag ccccgagggg cgcgcgatga tcgaatcgga
    13621 tatcgccaac gacggcgtca atgcggttct cgtggccgct tgttccccgc gcgttcacca
    13681 aaacacgttc cacttcgggc catcgaccat cgtcgaccgc atcgccttgc gagaacacgt
    13741 agcgtacgtg ctgcctgccg gcgaggaaga cacgcagatg gcagccgatg actacgtgcg
    13801 catgggtgtc accaagatca tgaaaaccga gccgatggat ccggcggtta ccgacgttca
    13861 agcttcgatt ttggtggtgg gcggtggtgt caccggtctg tccgcggcgc tcgaaacggc
    13921 gcgcgcgggc gtgcgggtcg tcttggtcga aaaacaagaa aagcttggtg gatgggtcgc
    13981 atcgtgggga cgcaagtttc ccacgcgtcc tccctatcgt gaccttgtcg acaacgacat
    14041 cgtcaacacc atcgaagcgg tagaaaaaca tcccctcatc acggtgtaca aagcggcttc
    14101 cgttctatcc atttctggac agccagggga attcgacgtt cggattcgcg tgggcaacga
    14161 cgagaaaaag gaaaccatcg gcgccatcgt gcaagccacc ggctggcgtc cgtatgacgc
    14221 caccaaactg acgcatctgg gctacggcaa gagcaaagac gtcatcaccc atgtcgagct
    14281 ggaagagatg ctgtccaaaa cgggcaaact ggtccgacct tccaacggtg cggtgccccg
    14341 ctcggtggtc ttcgtgcaat gcgccggatc ccgggacccg aaccacttgc cctattgctc
    14401 cgcggtatgc tgcaggacat cgttgaagca agcacgcgtg attcgagaat tgtatcccga
    14461 tacgggtgtt accttgctgt acaaagacgt ccggacaccc gggcaatacg agctgtttta
    14521 caaaaacgcc caggatgatc ccggggtctt tctcgtcaag ggagaggtaa cgggtgttgc
    14581 cgtggatcaa aaaaccatga cggtatccat gacggattca ctgttgggcg atgcggtgga
    14641 ggtgggagca gacttggtgg tcctggccgt gggcatgacg ccggtttcgg aagaacccat
    14701 tctcaacctg cagtatcgtc gtggtggcga actgccggtg ggcaaatatg ccttcccgga
    14761 ttccgacttc atctgcttcc cctatgagac ccagcggacg ggcatttatg cggcggggtg
    14821 tgttcgtgag ccgatggatt cggatgcttg cagtgaggac gcggcggggg cctcgctcaa
    14881 ggcgattcaa tgcattgcct gcacggcgcg cggcgccgct gtccatcctc gcgcgggtga
    14941 tctcacctat ccggagtttt tcctcagtcg ttgcacccaa tgcaaacgtt gcaccgagga
    15001 gtgtcctttt ggaacgcttg atgaggatga aaagggaacc cccaaaccca atccgggccg
    15061 atgtcgccga tgcggcgtat gcatgggagc atgtcccgag cgcatcatct cgttcaaaaa
    15121 ctactcggtc gacatgatcg gttcgatgat caaggccatc gaagtccccg aagaagatga
    15181 ggaaaaaccg agatatgtcg ctttcgtatg cgaaaacgac atcattccgg ctctcgacct
    15241 atcctcttcg ctgcgcaaaa agatttcgcc gtgggtgcga ttcgtttcgt tgcgctgttt
    15301 gggttccacc aacttgcagt ggatcaacga ctcgctgatc aaaggaatcg acggggtcat
    15361 gttgatcgga tgcaaatcgg gagaggatta tcaatgtcac ttcatcaaag gaagtgagct
    15421 ttgtggcgtt cggatgtcca agatccaaga aacgttgact cgtcttgccc tcgagcccga
    15481 gcgcatcgcg gtcaaggaat tttccatggg agactgggag tcactgccag actggatcaa
    15541 cgagttcgtg gacaagacca atgaactcgg accgaatccc aacaaggggt tctgatcgac
    15601 agcatgcgaa agcggctgag aacgaggtta ttcacgcatc gaggagtgcg tcatgtccaa
    15661 gatcgagcgg gttgaccccg acttgaagtt cgttcgaaag atcgtcgaaa acggtggcgg
    15721 atcggtcaag cgctgttatc aatgcgccac ttgttcggtg gtatgtacct tgtctcccaa
    15781 ggacaaaccg tttccgcgaa aggaaatgct ttgggcacaa tggggattga aggatcggct
    15841 ggttggtgat ccggacatct ggctctgtca ccattgcgga gactgtacgg tgcattgccc
    15901 acgaggagca aagcccgggg agacgctgca ggcgatccga gcttccgtca tctcggagtt
    15961 gtcgttcccg aaagccatcg cggaggcggt caacaaacca caatacttcc ccgctttgct
    16021 gctattgccg atgctattgc tcggcgtcac gatgtgggaa gccggaacgc tcaaagcaca
    16081 tcctggcccg atcatctggg gcaatctgct cccccatagc gtctttttcg aaggtatgtt
    16141 catgctgctg atgggatgga gcgtcgttgc gggcggcgtg gggatcgtcc gtcaatggca
    16201 acacatgaag cgcatgtgtc cagcggacaa agacgccaag gggctaccga ttggggcagc
    16261 gttcgtgcag gccgtaaaag aactcctgtg gcattcgcgc tttgaggctt gcgggcaaaa
    16321 ccgggcgcgg acctggcccc atcgtctcct ctttttcggt ttcctatccg cgatgatcac
    16381 ggcaggaggg gctgccattc tcgaaaaagc tttccacatt cctccaccgc tacccaatcc
    16441 agacgccgct gcctgggcgt acgccgtggg aatgttcttc aaaatcctag ggatactcgg
    16501 tgccgtcggg ttactcgtgg gttcgggatg gttgctcaac cggcgcatgg tcaaccaaga
    16561 ccaaacgggg gattc
//
LOCUS       DAOSOR010000046        16502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_3543_length_16502_cov_3.962060, whole genome shotgun sequence.
ACCESSION   DAOSOR010000046 DAOSOR010000000
VERSION     DAOSOR010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 16502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16502
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3543_length_16502_cov_3.962060"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3292)
                     /locus_tag="PK710_04155"
     CDS             complement(<1..3292)
                     /locus_tag="PK710_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOT08937.1"
                     /translation="MRRIVVLGVLLAMMLAITALQTSQSAAHDPMTLAAIGFVILAAY
                     TVSEVGSLLQLPQVTGYIVAGLALGPAAANILSSEVVVEMKMFNTLALGLIATGAGLE
                     LDVSRLRRVWKTLIATVGVKVILSGSFVIATLLAVQTALGSLELGSKAELISLSLVLG
                     VLSLTTSPAIVMAVLNETKSKGRMTDLALGAAVLKDLVVVTLLALVVAVSRTLLAPDA
                     EVDARVIINVGKEIASSIAAGGVLGVLLILYIRYVRAEMLLFVAAMILVVSELGRALH
                     LEILLVFITAGFVVRNFSHHEEELASPLSLVSLPVFVLFFTNAGASVDLRTTLTILPL
                     ALAICAARAIAFYLSARAGGAVGQEDFQVRRFAWLGYLPQAGVTLGLVGLAAQQLPDL
                     AGPISTTGMAMVAINLLIGPISLRHALRATGDIEGVPASRRSSAPSSKHPSVIPEEPL
                     EKAEADAAASIERATAAIDEEKNEKLRSMAQQTHTHLRQSVDGFYRDLLEPWAATFRS
                     ELRDSLTMIAKDRGSPQVWLESITLPDVSAQGETCKKLFESLRSPISSLPDHVTADLD
                     ASQLAVSSDDSIALFIRKRFRSLWILAFRQPAVRTIPVRLVARSSLEPSLSAISAAVL
                     ESWYAMQAGMLHQLSAFASDSESLAHVLQRTDEQMETWLGRVRASLDMAVSTAMTTMT
                     RTLSEVGSPTMPTTTLRYSTVEPQVREAIRKLHEEPSAWLSKMEAARAAPYLTACLAR
                     LDRTTRDAVDRHISQRSESAAQTMVPIILAVRERIATIRNNIENPDLVLDVKAIQQAC
                     REAYPEREELQIDRVGDLRPTASIHNVSLDLRAVVRELPEELTVLPPSVNLQDVQRAR
                     DVVTRTIHFRALAAHHTLWSFLPPLDDCVQRASLSLSRSNGRVREAIDWALDSVDGIG
                     DGPDDEMPHVEEFLSALEEVEQRLRELEADVHGTTEGLQREVMDCVTSVSRAMLADAT
                     GQKAEGPELPGASTWSAQLRQRLSILVAPAKMRMLRYRARAMEIAKRFGGSALSKDLR
                     LRLEKPVLDAVSIHKYVSRWRAPLAIPDTYARLFEDQPVRDYRRFTANEASYEALLEA
                     ERAWEQGSP"
     gene            complement(3289..4569)
                     /locus_tag="PK710_04160"
     CDS             complement(3289..4569)
                     /locus_tag="PK710_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08938.1"
                     /translation="MSVTILLVLVVIFSFAFGHLLTRYVSRYIALSGAEYLLVGLLIG
                     PQVPPRLMTHDSLALLEPFIALLLGLVGFVVALHTRDVFRNLRITMVGLVSATFVLLI
                     VAGAMLFLADRLLVPAPGSLHFEWEIFRTQRHALVLTLSTSHLWMSLALGAAACVASP
                     FVIDSAARMLKASGPVTELLHGSATLSQVLAVIVVGVAATTARSTDTATGVGLSAGMA
                     LLTAVVVGVICGLLFTLFIGQEKDAVRIFLATAGAVIFASGVGTALGLSPLFVNLVAG
                     ITVALTSRHTQRLRDELDRLQHPLFVLIMIFGGALWAPVEGWVWLLPIFYIAVRYAVR
                     RAFTRLTVRVVAPSHVLSPRIGLGLLSQGTMAVAIGVDFSQRFPHYSASVLTTVLAGT
                     LVSDLWSARSLRSVLLDAGESAHDSLPASTRRAS"
     gene            complement(4566..5456)
                     /locus_tag="PK710_04165"
     CDS             complement(4566..5456)
                     /locus_tag="PK710_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HOT08939.1"
                     /translation="MLGIGGSGADPSLSRDVQKALQQSMLALAEGSKPLAAVTRAVAA
                     LEDDGAFNAGRGSALRQDGLSILMDAMVWDSSGNFGAVTNLGLVRNPVKVALDVANSQ
                     LAMMGGPGATSFARAHGHPAFDAWSSRAQRQWEVASMADAGRERDGEGASDSQHSSVN
                     TIAYHNAAVVLRTAEGQFAGAASGGGSSLALPGEIGAAGIPGAALFVGQNGAVAISGP
                     SPLLLRIQLARAVYEQMIESQSTTQAVVYGLEATPKDTTLAIVAVDRDTFRMGSRGPV
                     AWGFLPSAPNTPTPIQASEP"
     gene            5572..6039
                     /locus_tag="PK710_04170"
     CDS             5572..6039
                     /locus_tag="PK710_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08940.1"
                     /translation="MGAEEAAGAGAEALAKAEAVEAAGVVNEADGGAGGFSAEEMAAD
                     AWEFMGAKGTAPATEPEDASGIEASVWVSARLRSRQLPAPRLATASTKTAAITRFSGR
                     KRGDCRSFDRGEPHTMGAFYRPCGLMTIDGFRRSVEGGSGTVLSAAPERVNLS"
     gene            6072..7235
                     /locus_tag="PK710_04175"
     CDS             6072..7235
                     /locus_tag="PK710_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOT08941.1"
                     /translation="MIFVDCRSVLAYPTGLLAYQRSLAKHLPDIAPHEHFVWLRHKDA
                     PGRLSYRPNAHEVFVSGDPESTLAPWLLHKNLRMEGARLFHATASFFPSNLPIPVVTT
                     VHDLVWITNPSWLIRRGFKGKISSFLGRRYVIDALRHSAHIIVPSQATQREIDEIAPF
                     ASDRVTVIHHGVEERFKPLDREDDSAVARVHTIVSRLLGGAPRYVLDVGRAAVYRNHE
                     GLIKAFATAFKSDPDIHLAFVQAMCRQSRPIMRLAARLGLDGRVHLLSGVASRDLVAL
                     YQGAICLCHPTLHEAYGTVVAEAMACGCPVVTSGRAAAGEIAEGVAQLVNPEHEDEIA
                     AAMRRIAYERGVAKRMRTRGLARAASLDSRRMAQATMAVYEKVLQRPVQAVAG"
     gene            7380..9395
                     /locus_tag="PK710_04180"
     CDS             7380..9395
                     /locus_tag="PK710_04180"
                     /inference="COORDINATES: protein motif:HMM:NF024840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4114 domain-containing protein"
                     /protein_id="HOT08942.1"
                     /translation="MSRCSRSLLIGSLGAALLWNTPASAVITQVDGTVLPVSDRLQIA
                     LDRSVALGGEGTPGLLHAVFDADIVPEVFQIPKNSNGKYRVVEFFDIEEGAGYENTIG
                     WYNVSDPSTLYPVLNCAHEPPNKVNVDFQKEFDDGRYKGGFVGFFLVSPGSNGGCGNP
                     ANLGQPGSTAFIVYTEAQLNGDGNYVHYLIYQSKQNPLAYYFGFEDLWRGGDNDFEDM
                     AVKVTGLIKACEPSPEICDGKDNNCDGLVDNEPVDVGQACVTISGNKPGFGACQAGIL
                     ECASKGPGDTTKICVGEVGPGIEVCNGLDDDCNGIVDDNPTDPSLGKPCGGSDEGECQ
                     RGTYACLAGTIACVGVVGPSPELCDGKDNDCDGVVDGTALEPRQNCKNDGDCSTSAPY
                     CLPTVLGEMVCARGPIDVVGQCTVSGSTCPGVKRCENGTVICEQSDPGTPEICNGADD
                     NCNGLVDEGDPGGGAECGPGGISLEMARTGQCEPGIEHCLGGKIQCVGGRGPTPEICD
                     GLDNDCDGLTDETAECPGESICVEGKCVEPCAGGEFPCPAGLVCVDGYCVKLGGGTGG
                     SGHDAGTQDDGAAGQNGEAGVDASAGAAGTAGSGGTAGSGGGFRPDGGASPGPHEQSR
                     ENWGLVTGGGGVSCALGRATSSLPAAIVALFALAMAGRMSPRTGRKG"
     gene            9395..11863
                     /locus_tag="PK710_04185"
     CDS             9395..11863
                     /locus_tag="PK710_04185"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HOT08943.1"
                     /translation="MKIYTYLLLPLFAGIVSGSFACTTESYCFDCEHAAAGAAGSSGS
                     PGTGGSGASGVLDASFDNVFIDSNSCNADLDNDPLNCGFCGHVCEIPNAYAMCKDKFC
                     VIDRCASGYYDINGKVEDGCEYECEPLRDTQGNPVTEETLCNGIDDDCDGLIDEVFDL
                     ESDAQHCGGCNQACQAPANAIMKCVKGKCEFDSCFVGYEDVNGDIGNDPTDGCECNFL
                     GIEECNYNDDDCDGEVDEGFDLTSDVNNCGACQYACGDLYPHAKTICENGACAFGGCL
                     PGFHDVDGLLATGCEYECTPGNPAVEACNGIDDDCNGFVDDGVLPGVGESCGSNVGAC
                     KFGKQQCVGGSLICVGDTKPSTELCDGIDNDCNGKTDEICPTAKSPVRLDVGGSAQGQ
                     HSTTQLSMATQGDATFAAYLDRRMASNGADIRLNYTLTANGAWLTSDMVVAGTAASEV
                     EPWVFTSPTKLYVAYELFIAGDVRRIQLARTGISPTTWTTIQAEKSAPASSDSFYVRG
                     IVAGQAAGADQIVLVWQTLQGTNRDIYLQASNDGGVTWRASDLRVNAVAGKAELPDLA
                     SDGKGRAFVVWRDARGTVPEVYFATYDVASATLGANKKLSSGDATKPAVIAADDQNNV
                     HVAWTQLPSSGAKTIRVATSVNRGDTFVVGVVDNTPVFADADSPHIVARAGRAAVAWE
                     DNRSGLSDVYVNVWASGAWRSQAARADGGPRGAYRSTQPRIAFGAGNRLFVTYQEYRG
                     TGSNNQADVHVNFSLDGGVTFQPKDTRVDPGAVGLADSTSPRLSVPGTSGPGIHPIVI
                     WLDNFNGTTASQNADVYAARVEFP"
     gene            12122..13465
                     /gene="rsxC"
                     /locus_tag="PK710_04190"
     CDS             12122..13465
                     /gene="rsxC"
                     /locus_tag="PK710_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOT08944.1"
                     /translation="MRRTFRGGVHPRDHKELTCEKPIRSLPSPDKVVLPLRQHVGAPA
                     RCIVKVGDHVAQGQPIAEAVGHVSAPVHASMAGVVRTIGKHLHPAGFECEAVTLEAHA
                     EDDAKRPSSPYRLPNARPDYTNTDSEELRALIQSAGIVGAGGAGFPTHVKLAPPKDKP
                     VDTLIINAAECEPYLTCDHRTMLEESEIVAHGVRIAKRILGVDRVLVGIEDNKLDAVQ
                     VMKKAFANDPAVEVAVLSVKYPQGGEKQLIEALTGRQVPPPPGLPMDAGCVVQNITTC
                     AAIARAVMQGIPVFERVITLAGSMVNDPGNVRVPIGMLLSDVIDRVGGLAEPPSKVIM
                     GGPMMGLAMAELDVPIVKATSGFVFLSKNQVKTVLPSPCIRCGRCAAACPLHLQPGEI
                     ARWVELGNLDAASALHLMDCMECGTCSYICPSYRWLVQQIRLGKAKVHERKQPSA"
     gene            13440..14492
                     /locus_tag="PK710_04195"
     CDS             13440..14492
                     /locus_tag="PK710_04195"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOT08945.1"
                     /translation="MNENNPPPEPKAMEPVQPSPLRLLVGTSPHVHAEQGVPEIMRWV
                     VLALLPAVAVSLWSFGLEALRVYVLAVGACLAIEWICLRIFKTPGSLADGSAAVTGVL
                     LAMNLPPTSPSWMVLIGAVVAIVFAKHLFGGLGSNIFNPALTARVFLLISWPVSMTTW
                     LKVGERGFISPSADLQAITQPTPLGMLKVGELQSVATEAGEWLLRDEGGSLGEVSALA
                     LVLGGVCLLWKRIITWEIPVFFIGTTVVFTAAAWLIDPSSYASPLTHALSGGLLLGAI
                     FMATDMVTSPGTFQGRVIFAIGCGLLTAIIRLWGGYPEGVSFAILLMNGLTPLIDRLV
                     RPRPHGAMAPSRKVPA"
     gene            14489..15103
                     /locus_tag="PK710_04200"
     CDS             14489..15103
                     /locus_tag="PK710_04200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOT08946.1"
                     /translation="MKTALHYVGVLALICAMAAGLLATVHEITSEPIASSRKRVTLEA
                     VRTVLPPFETLADAETVKTIVTDPSAPEMAAAFSAGRLIGAAVKVTDPDGFGGDVTFM
                     VGVTSDAKVHAIRLLAHKETPGLGTKLGDPIFTKQFQGLAIPAGGLRVHKDGGTIQAI
                     TGATISSRTATRAATRAVEHFEKVKNQLAQAASQAQPAQGGPRG"
     gene            15096..15716
                     /locus_tag="PK710_04205"
     CDS             15096..15716
                     /locus_tag="PK710_04205"
                     /inference="COORDINATES: protein motif:HMM:NF009070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOT08947.1"
                     /translation="MAERTASQEFQKGLLRENPLFVLALGMCPSLAVTTSLFNGIGMG
                     LAATFVLVCSNAVISLLRNFIPKGVRIPAYIVVIASFVTVVEMLMEAYVFDLHQAPGV
                     FIPLIVVNCIILGRAEAFASRNGLWRSVLDGLGMGLGFTLALVSMGTVREILGNGTLL
                     SGTPFELTVLGEGFKANPVLVMVLPPGAFFTLAVLMAGANRWMRRA"
     gene            15719..16315
                     /gene="rsxA"
                     /locus_tag="PK710_04210"
     CDS             15719..16315
                     /gene="rsxA"
                     /locus_tag="PK710_04210"
                     /inference="COORDINATES: protein motif:HMM:NF003481.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOT08948.1"
                     /translation="MELSFGQILQLIVWAVFVNNFILAQFLGICPFLGVSKKTESAVG
                     MSLAVIFVMTVAAAVTWLLNQYVLVPLGLVFLKTILFILVIAALVQFVEMVIKKMSPA
                     LFQALGIFLPLITTNCAVLGAALLAIQKDFSFFTTLIYAFASSLGFGLVMVLFAGIRE
                     RMDTTIETPAGMRGLPVTLVAAGILSLAFLGFAGLVKM"
BASE COUNT         3427 a         4675 c         4982 g         3418 t
ORIGIN      
        1 ccggagagcc ttgttcccac gctcgttccg cttccaacaa ggcttcgtag gatgcctcgt
       61 tggcggtaaa acgacgataa tcgcgtacgg gttggtcctc gaagagtcgc gcgtaagtat
      121 ccggaatcgc aagcggagcg cgccagcgac tcacgtactt atgaatgctg accgcatcga
      181 gcaccggttt ttccaaacgt aaacgaaggt ctttggacag cgcgctgcct ccgaatcgct
      241 tcgcgatctc catcgcccgc gcgcgatagc gcagcattcg cattttggcg ggagctacca
      301 aaatggatag ccgttgccgt agctgcgctg accatgtcga tgccccgggt agctccggtc
      361 cctccgcttt ttgccccgtg gcatcggcca acatggcccg ggataccgac gtcacgcaat
      421 ccatcacctc gcgctgcaac ccttcggtcg tgccgtgaac gtcggcttcc aactcccgca
      481 accgctgttc gacttcctcc aacgccgaca aaaactcctc cacatgcggc atttcgtcat
      541 ccggaccgtc accaatgccg tccaccgaat ccaacgccca gtcgatggcc tctcgtaccc
      601 gcccgttgga gcgagaaagc gaaagggaag ctcgttgcac gcaatcgtcg agcggtggca
      661 aaaatgacca aagcgtatga tgggccgcca acgcgcgaaa atgaatcgtc cgggtgacaa
      721 cgtccctggc gcgctgcacg tcctgaagat tcacagaagg gggaagaacc gtgagttctt
      781 ccggtaattc gcggacaacg gctctgagat cgagcgagac attgtgaatg gaagcggtgg
      841 gacgcagatc tcccacgcga tcgatctgaa gctcctctcg ttcggggtag gcttcacgac
      901 acgcctgctg gatggccttc acatccagga cgagatcagg attttctatg ttatttcgaa
      961 tagttgcaat tctttcgcgc accgccagga tgatgggcac catcgtctgc gccgcgcttt
     1021 cggaacgttg gctgatatga cgatctacgg catcgcgtgt cgttcgatcg agccgtgcca
     1081 ggcatgcggt gaggtaggga gccgcgcgag ccgcttccat tttcgagagc caggcactcg
     1141 gctcttcgtg caatttgcga atggcttcac gcacttgggg ctctacggtc gaataccgaa
     1201 gcgtggtggt gggcatcgtg ggagagccaa cctccgagag cgttcgcgtc atggtcgtca
     1261 ttgccgtaga tacggccata tccagggaag cgcgaacacg gccgagccat gtttccattt
     1321 gctcatcggt tcgttgcagg acgtgggcga gggattcgga gtcgctggcg aacgcgctca
     1381 attgatgaag catgcctgcc tgcatggcgt accaggattc caggaccgca gcgctgatgg
     1441 ccgaaagcga aggttcgagc gaggatcgag ccaccaggcg cacgggaatc gtgcgaacgg
     1501 caggttgtcg aaaagcgagt atccataacg aacgaaagcg tttgcggatg aatagggcga
     1561 tggaatcatc cgacgatacg gctagttgcg aagcgtcgag gtccgcggtg acatggtcgg
     1621 gaagcgacga aatgggcgag cgcaaggatt cgaagagttt tttgcaggtt tcgccttgtg
     1681 ccgaaacatc gggaagggtg atggattcga gccagacctg gggggagcct ctgtctttgg
     1741 cgatcatcgt tagcgaatcg cgcaattcgg agcgaaaggt ggctgcccag ggttcgagga
     1801 gatcgcgata aaaaccatcg acggactggc gaagatgcgt gtgcgtctgt tgagccatcg
     1861 aacgaagctt ttcgtttttt tcctcgtcga tggcggcggt agcccgttcg atggaagcag
     1921 cggcatccgc ttccgctttt tcgagtggtt cttcgggaat gacgctggga tgtttgctgg
     1981 atggtgcgga ggagcggcgg ctggcaggaa cgccttcgat atccccggtg gcccgaaggg
     2041 catggcggag gctgatgggg ccaatgagca agttgatggc gaccatggcc atgcccgtgg
     2101 tcgagatagg tccggccagg tcgggcaatt gttgcgcggc gagtcctacc aaaccaagag
     2161 tgacaccggc ttgtgggagg tagcccagcc aggcgaagcg acggacctgg aagtcttcct
     2221 ggccgacggc gccgcctgct cttgcgctga gataaaaagc gatggctcgc gcggcgcaaa
     2281 tcgccaaagc gaggggaaga atcgtgaggg tggttcgcaa gtcgacactg gcgcccgcat
     2341 tggtgaaaaa cagcacgaac acggggagcg acacgagaga aagcggcgaa gccaactctt
     2401 cttcatggtg cgaaaagttg cgcacgacga aaccggcggt gatgaatacg agcaggattt
     2461 cgagatgaag ggcacggccg agttcagaga ccaccaaaat catggccgcc acgaaaagca
     2521 gcatttccgc gcggacataa cgaatataaa gaatgagaag cacgcccaaa acgccgcctg
     2581 ccgcgatcga actcgcaatc tccttgccga cattgatgat gacccgcgca tcgacttccg
     2641 catccggcgc aagcaatgtg cgcgataccg caaccaccaa cgccaacaac gtcacgacca
     2701 ccaagtcctt gagtaccgcc gctccaaggg ccaggtccgt catccgtccc ttggacttcg
     2761 tttcattgag aaccgccatc acgatggcgg gtgatgtggt caacgacaaa acaccgagca
     2821 ccaacgaaag agaaatcagc tctgctttcg accccaactc gagggaaccc aacgcggttt
     2881 gcaccgcaag cagcgtggcg atcacaaaac tcccactcag gattaccttt acgcccaccg
     2941 tggcgatcaa ggttttccaa acgcgtcgca accggctcac gtcgagttcc aatcccgccc
     3001 ccgtggcaat caagccaagg gcaagggtgt tgaacatctt catctcgaca accacttccg
     3061 acgacaggat gttcgccgcc gcgggaccca acgccagacc ggctacgata tatcccgtca
     3121 cttgcggaag ctggagcaga ctgcccacct ccgaaaccgt ataagccgcg aggatcacga
     3181 agccgatggc cgccagtgtc atgggatcat gggctgcgct ttgcgacgtt tgcaacgccg
     3241 tgatggcgag catcatcgcg agcagcacac caagaacgac gattcttctc atgacgcccg
     3301 cctcgtcgaa gctggtagtg agtcatgggc cgattcacct gcatcgagca aaaccgaccg
     3361 caacgagcgc gcgctccaca aatcactgac gagggttccc gccaatacgg ttgtcaaaac
     3421 actggcggaa taatgcggaa aacgctgaga aaaatcgact cctatggcca ccgccatggt
     3481 tccttgggac agaagtccca gacctattct tggcgatagg acatggctcg gagctaccac
     3541 gcggacggtc aaacgcgtga aagctcgtcg gaccgcgtaa cgcacggcga tatagaagat
     3601 tggcaataac cagacccagc cttcgacagg agcccaaagc gctccgccga aaatcatgat
     3661 gagcacgaac aacgggtgct ggagacgatc caattcatcg cgcaaccttt gggtgtgtcg
     3721 cgaggtgagt gcgacggtga tacctgccac gaggttgacg aatagcggcg atagtccgag
     3781 ggccgttccc acgcccgagg cgaaaatgac cgctcccgcc gtggcaagaa agatgcgcac
     3841 ggcgtctttt tcttgtccaa tgaatagcgt gaataaaagc ccgcagatga ctcccacgac
     3901 gaccgcggtc agaagcgcca ttccggcgga aagcccaact cctgtcgcgg tgtctgtcga
     3961 tcgagcggtg gttgcggcca cgcccacgac gatcacggcg agcacctgac tcaacgtcgc
     4021 ggagccgtgg agcaattctg tcaccggacc gctcgctttg agcattcgag ccgcggaatc
     4081 gattacgaaa ggagaggcta cgcacgcggc ggcgcccaac gccaacgaca tccaaagatg
     4141 actcgtggac aaggtcagca cgagcgcgtg gcgctgggtt cgaaagatct cccactcgaa
     4201 gtgcaacgag cccggcgccg gcacaaggag acgatccgcc aaaaacagca tggctccggc
     4261 aacgatgagc aatacgaacg tcgcggaaac aagacccacc atcgtgattc gcaggtttcg
     4321 aaatacatcg cgggtgtgca gagcaaccac gaatcccacc aaaccaagca ataacgcgat
     4381 gaatggctcc agcaacgcca gagagtcatg ggtcatgagc cggggaggca cttgagggcc
     4441 gatcaacaga cccacgagca agtattccgc accggacaag gcgatgtaac gcgatacgta
     4501 gcgagtcagt aaatgcccga aagcaaagct gaaaatgacg accaggacga gcaggatggt
     4561 cacgctcatg gttcactcgc ctggataggg gtaggcgtat tcggggcgct gggcaaaaag
     4621 ccccaagcga caggtccgcg cgagcccatg cggaacgtgt cgcgatcgac tgcgacgatg
     4681 gctagggtcg tatctttggg cgtagcttcg agaccgtaga caacggcttg ggtcgtggat
     4741 tggctttcga tcatctgctc gtacaccgcg cgagcaagct ggatacgaag gagcaaggga
     4801 cttgggccgc tgatcgcgac cgctccgttt tgtcccacga acaacgcagc gcccgggatg
     4861 ccggccgctc cgatttctcc gggtagcgca agggatgagc ctcccccgct cgccgccccc
     4921 gcaaattgcc cttcggcggt ccgaagcact accgcggcat tgtggtaagc tattgtattt
     4981 acagatgaat gttgagaatc ggaggccccc tctccgtccc gctcccgacc tgcatccgcc
     5041 atcgaggcca cttcccattg acgctgcgcg cgcgaagacc aggcgtcgaa tgcgggatga
     5101 ccgtgggcgc gagcaaacga cgtcgcaccg gggcctccca tcatcgcaag ctgtgaatta
     5161 gcgacatcca gagcaacttt gacagggttt cgcaccaacc ccagattcgt taccgctccg
     5221 aaattgccgc tcgagtccca aaccatggcg tccatcagga tcgataaccc atcctggcga
     5281 agtgctgacc ctcgtccagc attgaaagcg ccatcgtctt ccaacgcggc aacggctctg
     5341 gttaccgcgg ccagaggctt ggagccttcg gcgagtgcaa gcatgctttg ctgcaaagct
     5401 ttttgaacgt ctcgactcag gcttgggtct gcaccggagc ctccgattcc gagcacgacc
     5461 catcctgaat gtcccggtgt tgcctttgac ggctgggaag gcgcggagga atgcgggggt
     5521 ggtgtactag agcttgcttc gggcaaaagg agagcggttg aggtatccgg tgtgggagcc
     5581 gaagaggcag cgggagctgg cgcggaagca cttgccaagg cagaagcggt agaagcagca
     5641 ggagtcgtca acgaagcgga tggaggcgcc ggcggcttta gcgcggaaga aatggcagcg
     5701 gacgcgtggg agttcatggg agccaaaggg acagccccag ccacggaacc agaggatgcg
     5761 tctggcatcg aagcctccgt ttgggtttcc gccagactgc gaagtcgaca gcttcccgct
     5821 cccaggcttg ccacggccag cacaaaaaca gccgccatca cgcgtttttc cggacgaaag
     5881 cgcggggatt gtcgctcgtt cgatcggggt gagccacaca cgatgggcgc attctatcgt
     5941 ccatgcgggc tcatgactat cgacggcttt cgaagaagcg tcgaaggggg gagcggaacg
     6001 gtattgtccg cagcgccaga gcgtgtaaat ctgtcctaaa ctcggtgaac gggctttcgg
     6061 agtgatacag gatgatcttt gtcgattgcc gaagtgtgtt ggcgtatccg acgggattgt
     6121 tggcctacca acgatctttg gcgaagcatc ttccggatat tgcgccccac gagcacttcg
     6181 tgtggttgcg ccacaaggat gcgccgggac gattgagcta tcgtcctaac gcccacgaag
     6241 tattcgtttc cggagacccg gagagcacgt tggctccgtg gcttttgcac aaaaatctcc
     6301 gtatggaagg ggcgcgccta tttcacgcca ccgccagctt tttcccatcc aatctaccaa
     6361 ttcccgtcgt gacgaccgtc catgacctcg tgtggattac gaacccttcc tggttgatcc
     6421 gtcggggatt caaagggaaa atatcttcgt ttctgggtcg tcgatacgta atcgatgcct
     6481 tgcgccattc ggcgcatatc attgtgccaa gccaggcaac ccaacgagag atcgatgaaa
     6541 ttgctccatt tgcttccgat cgcgtgacgg tcattcatca tggggtggaa gagaggttca
     6601 agcctctcga tcgcgaggat gattccgcgg tggcgcgcgt ccatacgatt gtgtcgaggt
     6661 tattgggggg agcgccgcgt tacgttctcg acgtggggcg agctgcggtt tatcgaaatc
     6721 atgaaggctt gatcaaagcg tttgccacgg cattcaaaag cgatcccgac attcatctgg
     6781 cttttgttca agccatgtgc cgccagtcca ggccgatcat gcgcctggcc gctcgtttgg
     6841 gtctcgatgg ccgtgtccac ctgctcagcg gtgtagcctc ccgagacctc gtagccctgt
     6901 atcaaggtgc gatctgcttg tgccatccca ccttgcacga ggcttatggc accgtggtcg
     6961 ctgaggccat ggcttgcggt tgtcccgtcg tgacttccgg aagagccgcc gcgggagaga
     7021 ttgcggaagg cgtggcgcaa ctcgtcaatc ctgagcacga agacgaaatc gcagcagcca
     7081 tgcgtcgcat cgcctacgaa cggggtgtgg caaaaaggat gcgaactcgc ggacttgcac
     7141 gcgccgccag cctcgattcc cgtcgtatgg cccaggccac catggcggta tacgaaaaag
     7201 tgctacagcg gcctgttcag gccgttgccg gataaaaaca cgacacccag gcgctcgctg
     7261 ttgccagcac ctctggaaat ctcaggatga tagattcacc ttgtaggtgc aggtggttcg
     7321 cggatcgatc tgccgattcg aggctgccga tgggatggat ttcacgctac actacgcgaa
     7381 tgtcgcgctg ttctcgttct ttgctcatag gtagcctcgg ggcggccttg ttgtggaaca
     7441 ctcccgcctc ggccgtgatc acccaggtgg atggaaccgt cctgccggtt agcgatcgcc
     7501 ttcaaattgc gctcgatcgc tcggtggcgc tgggggggga ggggactcca gggctccttc
     7561 atgccgtttt cgatgcggat atcgtgccgg aggtctttca aatccccaaa aatagcaatg
     7621 ggaagtatcg cgtggtggag tttttcgaca tcgaagaggg cgcgggatac gaaaatacca
     7681 tcggttggta caacgtctcc gacccctcca ccctctatcc cgtgttgaac tgcgctcatg
     7741 aaccaccgaa caaagtcaat gtcgatttcc aaaaagaatt cgatgatggc cgatacaaag
     7801 gaggattcgt gggatttttc cttgtgtcgc cgggaagcaa tggcggatgt ggcaaccccg
     7861 ccaacctcgg gcagcccggt agcacagcgt tcatcgtcta taccgaagcg cagctcaacg
     7921 gtgatggcaa ctacgtccac tatctcatct accaatccaa acaaaacccc ttggcctatt
     7981 acttcggctt cgaggatctt tggcgtggcg gcgacaacga cttcgaagac atggcggtca
     8041 aggtcacggg cctcatcaaa gcctgcgaac catcacctga gatttgtgat ggaaaagaca
     8101 ataactgtga tggcctggtc gacaacgagc ccgtggacgt gggtcaagct tgcgtgacca
     8161 tttcgggcaa caaacctggc ttcggcgctt gtcaagcagg gattctggaa tgtgcaagca
     8221 aggggcctgg ggatacgacc aaaatatgtg ttggtgaggt cggccctggt atcgaagtgt
     8281 gcaacggtct ggatgacgat tgtaacggga tcgtcgacga caacccaacc gatccgtcgc
     8341 ttggaaaacc gtgtgggggc tcggatgaag gggaatgcca gcgaggcacc tatgcgtgct
     8401 tggcgggcac cattgcgtgt gtgggcgtag tgggaccttc accagagctt tgtgatggca
     8461 aggacaacga ttgcgatggg gtggtggatg gcacggcgct ggagccgagg caaaactgca
     8521 aaaacgatgg ggattgctcg acgagcgcgc cgtattgtct cccgaccgta ctcggagaaa
     8581 tggtttgtgc gcgcggtccc atcgatgtgg tggggcaatg taccgtatcg gggtcgacct
     8641 gccctggggt caaacgatgt gagaacggta cagtcatttg tgagcaatcc gatccgggaa
     8701 ccccagaaat ctgcaatggc gcggatgata attgtaacgg gctggtggat gagggagatc
     8761 caggaggtgg cgcggagtgt ggtccaggag gaatttcgct ggaaatggcg aggaccgggc
     8821 aatgtgaacc ggggatcgaa cattgtttgg gtggaaagat tcagtgtgtc gggggaaggg
     8881 gccccacgcc cgaaatctgt gatggtctcg acaacgattg tgatggtctc accgatgaga
     8941 cggcggaatg tcctggagag agcatttgtg tggaaggaaa gtgcgtggag ccttgcgccg
     9001 gcggcgaatt cccttgtccc gccggactgg tttgcgtgga cggatattgt gtcaaattgg
     9061 gtgggggtac cggaggctct ggtcacgatg cgggaacgca ggacgatggt gcggcaggac
     9121 aaaatgggga agctggtgtg gacgcttcgg ctggcgcagc agggacagcc ggctccggag
     9181 ggacagccgg ttccggaggg ggattccgac cggatggggg agcctcacca gggccacacg
     9241 agcagagccg agaaaattgg ggattggtga cgggcggcgg tggcgtgtcc tgtgcccttg
     9301 gacgggccac ctcttcgcta ccggctgcca tcgttgccct ctttgctctc gccatggcag
     9361 gacgaatgag ccctcgtacc gggaggaaag gataatgaaa atctatactt acctactcct
     9421 cccgttgttc gcgggcattg tgtccggctc ctttgcttgc accaccgaat cgtattgttt
     9481 cgattgcgaa cacgcggcgg ccggggcggc tggttcctct ggctctcccg gaacgggcgg
     9541 cagcggtgca agcggcgtcc tcgatgcctc cttcgacaac gtcttcatcg attccaattc
     9601 gtgtaacgcc gacctcgaca acgatccgct caattgtgga ttctgcggcc acgtttgcga
     9661 aatccccaac gcgtatgcca tgtgcaagga caaattctgc gtcatcgatc gctgcgcttc
     9721 gggctattac gatatcaatg gcaaggtcga agacggatgc gaatacgaat gcgaaccttt
     9781 gcgcgatacc cagggcaatc ccgttaccga agaaacgctt tgcaatggca tcgatgacga
     9841 ttgcgatggc ttgatcgacg aggttttcga tctcgaaagc gacgcgcagc attgtggcgg
     9901 ctgcaatcaa gcttgccagg cccccgccaa tgccatcatg aagtgcgtga aaggcaagtg
     9961 cgagttcgat tcctgtttcg ttggctatga agacgtcaac ggcgacatcg gaaacgatcc
    10021 gaccgacggt tgcgagtgca actttcttgg catcgaggag tgcaattaca acgacgacga
    10081 ttgtgatggg gaggtggacg aaggtttcga tctgacctcc gacgtgaaca attgtggggc
    10141 ttgtcaatat gcttgcgggg atttgtatcc gcatgccaag acgatttgtg agaacggggc
    10201 ctgtgctttc ggcggttgtt taccgggatt tcatgatgtc gacggcttgc ttgccaccgg
    10261 atgcgagtat gaatgtacgc ctggtaatcc cgcggtggag gcgtgcaatg gcatcgacga
    10321 tgattgcaac ggttttgtgg acgatggcgt attgcccggg gttggggaat cctgtggaag
    10381 caatgtcggc gcgtgcaagt tcggcaagca acaatgcgtg ggtggttcgc tcatttgtgt
    10441 aggggatacg aaaccgtcga ccgagttatg cgatgggatc gacaacgact gtaacggaaa
    10501 gacggatgag atttgtccaa ccgccaagtc tcccgtgcgg ttggatgtag gtggttcggc
    10561 gcaaggccag cattcgacga cgcaactcag catggcgacg caaggcgatg ctacgtttgc
    10621 tgcgtacttg gatcgaagaa tggcaagcaa tggggcggat attcggctca actacacctt
    10681 gacggccaac ggggcatggt tgacctcgga catggtggtg gcgggcacgg cagccagcga
    10741 ggtggagccg tgggttttca cttcgcctac caagctctat gtggcgtatg aactcttcat
    10801 tgccggagac gtgcgtcgta tccaactcgc ccgaacgggg atttcaccta cgacctggac
    10861 caccatacaa gcggaaaaaa gcgctccggc ctcatcggat tccttttatg ttcgcggtat
    10921 cgtggcgggt caagccgcgg gagccgacca aatcgtgttg gtatggcaga cgcttcaagg
    10981 cacgaatcgt gacatttact tgcaggcgtc caacgatggt ggcgtaacat ggcgagcgag
    11041 cgaccttcgc gtcaatgccg tcgcgggaaa ggcggaactg ccggatctcg cttccgatgg
    11101 gaaaggacgg gcgttcgtgg tgtggcggga tgcgcgaggc accgtaccgg aagtgtattt
    11161 cgctacctat gatgtggcgt cggcaaccct gggagccaac aaaaagcttt cttcggggga
    11221 tgccacgaag cctgctgtga tcgctgcgga tgaccaaaac aatgtgcatg tggcgtggac
    11281 gcagcttcct tcttcgggcg cgaaaaccat acgtgtggcg acgagtgtga atcgtggcga
    11341 tacgtttgtt gttggggtgg tcgacaatac gccagttttt gccgatgcgg attctccgca
    11401 tatcgtggcg agggcggggc gcgctgccgt ggcttgggaa gacaatcgtt cgggtctatc
    11461 ggatgtttat gtcaacgtat gggcatcggg agcttggcgt tcccaggctg ctcgcgccga
    11521 tgggggacct cggggagcct atcgctccac gcaacctcgt attgccttcg gggctggcaa
    11581 ccgattgttc gtgacgtacc aggagtatcg aggcactggc agcaacaacc aggcggatgt
    11641 tcacgtgaac ttctctctcg atggtggcgt cacattccag cccaaggata cgagggtcga
    11701 cccaggggcg gttggattgg cggattccac ttccccaagg ctcagcgtac ctggaacttc
    11761 gggacccggc attcacccga ttgtcatctg gctcgacaac ttcaatggca cgaccgcttc
    11821 gcagaatgcg gatgtgtacg cagcacgcgt cgaattccct tgagtcctcc ccgatacgcg
    11881 tcgcgcgtgc cagcgtgagc gcggctccct gcaaccgttc gaattccgat tcgcctgtag
    11941 tggtgtttgg gaccgcgtgt ggcgtaaacc atggaaacga ctgactttta ttgatttttc
    12001 cccacggaag gggataagga caaggtcatg aagccgtagg ggagtgcgag tgtttttcgt
    12061 ggaaacgctc agcatatcgc ccctgcgcac gatgttcggc tgaccaactt gggatagaca
    12121 catgaggcga acgtttcgtg gtggagttca tcctcgcgac cacaaggagc tgacttgtga
    12181 aaaaccgatc cgttccttac ccagccccga caaggtcgtg ctccccttgc gtcagcatgt
    12241 cggcgcgccg gcccgatgta tcgtcaaggt aggcgatcat gtcgcgcaag gacagcccat
    12301 cgccgaagcc gtgggccatg tctctgctcc tgtgcatgcc tcgatggccg gtgtggttcg
    12361 gaccatcgga aagcacttgc atcccgccgg tttcgaatgc gaagccgtga cgttggaagc
    12421 gcatgccgaa gacgatgcaa agcgcccctc ttccccttat cgattgccga acgccagacc
    12481 tgactatacg aatacggata gtgaggagct tcgagccctc atccaatctg ccgggatcgt
    12541 gggggccgga ggcgccggct tccccaccca cgtcaagctc gcccctccca aagacaaacc
    12601 cgtcgacacg ctgatcatca acgctgccga atgcgagcct tatctgacgt gcgatcaccg
    12661 caccatgctc gaagaatccg aaatcgtcgc ccatggagtc cgcatcgcaa agcgcattct
    12721 cggtgtcgat cgcgtgcttg tcggtatcga agacaacaag ctcgatgccg tgcaagtcat
    12781 gaaaaaggct ttcgccaacg atcccgcggt cgaggtcgcc gttctttccg tcaaataccc
    12841 tcagggcggt gaaaaacaac ttatcgaagc gttgaccgga cgacaggtgc ctccgcctcc
    12901 ggggctgccc atggatgcgg gttgtgtcgt acaaaacatc acgacctgcg ccgccatcgc
    12961 acgcgccgtg atgcaaggga ttcccgtatt cgaacgggtg atcacgttgg ccggttcgat
    13021 ggttaacgac ccgggcaatg ttcgtgtccc tatcgggatg ctgctgagcg atgtcatcga
    13081 ccgggtgggc ggcctcgccg agccaccttc gaaagtgatc atgggcggtc cgatgatggg
    13141 ccttgccatg gccgaactcg atgtgcccat cgtcaaagcg acctcgggat tcgtgttttt
    13201 atcgaaaaat caggtcaaaa ccgtacttcc cagtccgtgt atccgctgcg gacgttgcgc
    13261 cgcggcttgt cctcttcatt tgcaaccggg ggaaatcgcg cgctgggtcg agctgggaaa
    13321 cctggacgcc gccagcgctc tgcacctgat ggactgcatg gaatgcggca cgtgcagcta
    13381 tatctgtccc tcctatcgct ggctggttca gcaaatccgg cttggtaagg caaaagtcca
    13441 tgaacgaaaa caaccctccg cctgagccga aggccatgga acctgtccaa ccatcgcccc
    13501 ttcggctttt ggttgggacc tctccccacg tgcatgccga gcaaggcgtc ccggagatca
    13561 tgcgctgggt cgtgctggct ctgctgcctg ccgtggccgt ctcgttatgg tccttcggcc
    13621 tcgaggcgtt gcgggtttac gttctcgccg tgggcgcttg cttggctatc gagtggattt
    13681 gcctccggat tttcaagact cccgggagcc tggccgatgg atccgccgcc gtgaccggcg
    13741 ttcttctcgc catgaatctg cctcccacct cgccgtcgtg gatggtgctc atcggcgcgg
    13801 tggtggccat cgttttcgca aagcatctgt ttggcggttt gggcagcaat attttcaatc
    13861 ccgcgctcac cgcccgtgta tttctactca tttcctggcc cgtttccatg accacctggc
    13921 tcaaagtcgg cgagcgaggc ttcatttctc caagcgcgga cctgcaagcc atcacccaac
    13981 ccacccccct tggcatgctg aaggtgggcg aacttcaatc ggtcgccacg gaagctggag
    14041 agtggctctt gcgcgatgaa ggaggctccc ttggcgaggt atccgcactg gcgttggtgc
    14101 tcggcggggt gtgcttgctc tggaaacgca tcatcacctg ggaaatccct gtctttttca
    14161 taggcaccac cgttgtattc accgccgcgg cttggctcat cgacccatcc tcatacgcct
    14221 ctccgctcac ccacgcgctg tcgggtggct tgctgctcgg cgccatcttc atggccaccg
    14281 atatggtgac ctctcccgga accttccaag gacgcgtgat tttcgccatc ggatgcgggc
    14341 tgctgaccgc catcattcgg ctctggggcg gctatcccga aggggttagt ttcgccatcc
    14401 tcctgatgaa tggcctcacc ccgctcatcg ataggctcgt tcgcccacga ccccacggcg
    14461 cgatggcgcc gagccgcaag gtgccagcat gaaaaccgcg cttcattatg tcggtgtgtt
    14521 ggcgctgatt tgcgccatgg ccgccggatt gctcgccacc gtccacgaaa tcacctccga
    14581 acccatcgct tcttcccgaa aacgggtcac cctggaagcc gtccgcacgg tgcttccccc
    14641 tttcgaaacc ctagcggacg ccgaaaccgt caaaaccatc gtgaccgacc catcggctcc
    14701 cgaaatggca gccgcctttt ccgctggtcg actcatcgga gctgccgtga aagtaaccga
    14761 cccggatggt tttggcggcg atgtaacgtt catggtgggg gtgacctccg acgccaaagt
    14821 ccatgcgatt cggctacttg cccacaagga aaccccaggc cttggcacca agctcgggga
    14881 tccaatattt accaagcaat ttcaaggact tgcgattcca gcgggaggcc tgcgcgtcca
    14941 caaagacggc ggcaccatcc aggcgatcac gggtgcgacc atttcgagtc ggacggcgac
    15001 tcgtgcggcg actcgtgcgg tcgagcactt cgaaaaagta aaaaatcagc tagcgcaggc
    15061 cgcttcccag gcccaacccg cgcaaggagg tcctcgtggc tgaaaggacc gcttcccagg
    15121 aattccaaaa aggattgctc cgcgagaacc cccttttcgt tctcgccctc ggcatgtgtc
    15181 cttccctggc cgtgaccacc agcctgttca acggcatcgg catgggattg gccgcaacct
    15241 tcgtgctcgt ctgctccaat gccgtcatct ccttgctgcg aaacttcatt cccaaaggcg
    15301 ttcgcatccc agcttatatc gtcgtcatcg catcgttcgt caccgtcgtg gaaatgctca
    15361 tggaggcgta tgtcttcgac ttgcatcaag cccccggcgt gttcatcccc cttatcgtcg
    15421 tcaactgcat catcctcggc cgtgccgagg catttgcttc ccggaatgga ctgtggcggt
    15481 cggttctcga cggattgggg atggggcttg ggtttacgtt agcgcttgta tcgatgggga
    15541 cggtgcgtga aattcttgga aacgggacgc ttctttccgg cacgccgttc gagttgaccg
    15601 tgttgggtga gggattcaag gccaatccgg tgctcgtgat ggtgcttcct ccgggcgcgt
    15661 tctttacctt ggcggtgttg atggcggggg ccaaccgctg gatgaggagg gcctgaccat
    15721 ggaactatcc ttcggccaga ttctacagct tatcgtctgg gcggttttcg tcaacaactt
    15781 catcctcgcg caattccttg ggatttgccc tttcctgggg gttagcaaaa aaaccgaatc
    15841 cgccgtgggc atgagcttgg ccgtgatctt cgtcatgacc gtggctgctg ccgtcacgtg
    15901 gctgctgaat caatacgttc tcgtgccctt gggactggtt tttctcaaaa ccatcctttt
    15961 catccttgtc atcgccgctc tcgtgcaatt cgtggaaatg gtgatcaaga agatgtcgcc
    16021 cgcgctgttt caggctttgg gtatcttttt gcctctcatc accacgaatt gtgcggtgct
    16081 gggagcggcg ttgctcgcca ttcagaaaga tttttcgttc ttcaccaccc tgatttacgc
    16141 cttcgcttct tccctcggct tcgggctcgt catggtcctt tttgcgggca tccgtgagcg
    16201 gatggacacc accatcgaaa ccccggcagg aatgcggggg ttgcccgtca ccctggtggc
    16261 ggctggcatt ctttctctgg catttcttgg gtttgcgggc ctggtcaaga tgtgaggaga
    16321 gcatcaccgt ggattggaac accatcctga taagcacggg cacgctggga ggcatcggcg
    16381 cggccgcggc ggctttgctc gcgggagcct cccgagtcct gcgtgtagaa gaagatccgc
    16441 gcatcgaaca agtcaccgaa gccttgcctg gcgccaattg cggcggctgc gggttccctg
    16501 gc
//
LOCUS       DAOSOR010000047        16330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_3590_length_16330_cov_4.642151, whole genome shotgun sequence.
ACCESSION   DAOSOR010000047 DAOSOR010000000
VERSION     DAOSOR010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 16330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16330
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3590_length_16330_cov_4.642151"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PK710_04215"
     CDS             <1..695
                     /locus_tag="PK710_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOT08949.1"
                     /translation="RSRYCDYPAEVHQLPSVGGYVDPSLEAILALQPTLVIGARGPIG
                     RSLVDKLEAREIQCFFPPTESVEQILAMISGLAEHLDTPQAAIHLTTSIRERLHHLQL
                     QVQPLSSPRTLLVFGQSPISVAGPGSFPDEMLRLAGCKNAVERGSHYPTLGFETVIGL
                     DPDLIIDATMAAERSTTPIHAQKAGWSSVRAVREGNVVRLGDDRILRPGPRLADGVAL
                     LARHAHKGVSIP"
     gene            692..1450
                     /locus_tag="PK710_04220"
     CDS             692..1450
                     /locus_tag="PK710_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOT08950.1"
                     /translation="MSQRRGPKSRLDVLLVERGLAESRTRAQALVMARLVEVDGVTID
                     KAGTTVGVDAQVVVRPSGPTYVSRGGEKLAGALVAFAPHGLVVADAVAADFGASTGGF
                     VDCLLQHGAARVHAIDVGHGLLHQRLRQDPRVVMWERTNARYVTAKTLGESADLVVID
                     ASFIGLSKLLPAARDVLREQGQLVALIKPQFEAGRRQVTQGRGVIRDENLRMSVIGRM
                     LEHIAHDGFTVLADSACVIAGPKGNREHFVFARK"
     gene            complement(1483..3687)
                     /locus_tag="PK710_04225"
     CDS             complement(1483..3687)
                     /locus_tag="PK710_04225"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT08951.1"
                     /translation="MNPAFLTMGLMSGLLLFAGCTPADRKGPDETAEKQGAPAMAAEA
                     NRRSDEPARVHELLLLENTRAAGQVMDTDLTNADPRVRRSAVRVLARAESEIVRDKLL
                     ASLSDHDPEVLAWSAYGLGRICARDRERTTSRLVARSVSLGLEPPVAAGRLDPWVALA
                     GALGKCASVQAEQSLVSWLSGPKQRAEAAAMGLGDVVSQHRRLEEETAASLLRATRGD
                     AANDPLTDALFPFTRLKRAPVRVNEQLFEACRKRLGHDGSARVFVLRSLGTLGEEAVE
                     LLGTVLRSTSGYSPAERAEAARALGKIDSPAAQKELLRAIDELAPINDPVVLTSLVGP
                     GFGSLMTALGSIRVTGKKAVRSRGLDALTNLDTPPGAPASVVRRISMLRCAAARLGAG
                     SNASFPRLLSCDSQKGSVGALARLSVIDRDELVGSRATIWKSYLDPKVAVVVRQAAIK
                     MLSGHPEAKGIVPLVVAALSDESLGVVTEGALLIAASPERFSVKTEPFQADPKVAEAL
                     LQAMGRSWPKDAIETKGAIARACGALHLEQARSWLTSLCSDSNPTLRQHAKQALQSLG
                     GAPPTCEPSPSTTVSPAPELARLAPKTTLELDTEVGKVQLHLDPTFAPVAVTRIVQLA
                     RNRFYDDMAVHRVVPGFVVQFGDKQGDGYGGAGLEALRCENTPVAFRQGVVGMALGGR
                     DTGSSQIFVTLADSPHLNGEYAVVGTATGPWDVLTEGDRINKVTIKVDAAAP"
     gene            complement(3684..4262)
                     /locus_tag="PK710_04230"
     CDS             complement(3684..4262)
                     /locus_tag="PK710_04230"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOT08952.1"
                     /translation="MIRPKPGRVNLSLTIVVPQSVPPRYGRAVTARCDAIAVLGARVL
                     PSGLPSPALQRRLERARLAFEQGVAPVVVAAGGKRWWGHSEAVSMKRWLEQLGVPSDC
                     IELEQLSENTAENAFYLTRLANNRGWKRLAIITCDWHLPRATLDFRRCHMDVWPIPAI
                     TPARLPERAYYGVREWVCSRLDGARLWRRNSQ"
     gene            4315..5151
                     /locus_tag="PK710_04235"
     CDS             4315..5151
                     /locus_tag="PK710_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT08953.1"
                     /translation="MHIKFWGVRGSIAVPGRDTLEVGGNTSCVEVRCGDTLIVLDGGT
                     GIRLLGQELLGKLPVEAYIFFSHVHWDHIQGFPFFTPAFIPGNRFHLYGGNNVTHTLE
                     ETLAGQMDYPSFPVHLTDMAAQMDFHDLREGQVVTIGEGSDLIRISNARGNHPNGVFA
                     YRIDYHHRSVVYCTDTEHYDVVDVKLAGLSKNADVLIYDTMYLPEEYSGEKGGGPKTG
                     WGHSTYEQGIKLAQEANVQQLVLFHHDPLHTDAMVADKERRARESFPASIAAREGLVI
                     NL"
     gene            5494..7149
                     /locus_tag="PK710_04240"
     CDS             5494..7149
                     /locus_tag="PK710_04240"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOT08954.1"
                     /translation="MRIAAQARTQLAQPPHPVDPIQFATYTADWLDPHGLWSAAPDSP
                     IGSALQQSASLLLSSLQNPNDPRGCPTAVALGSELARWVQNLRTVFDENLRVPSTPSD
                     PHRAATSALFENDSTPALGLAAELGQRLAALQPIGPFASHIQNAIRIRMFPHLSANDW
                     GDVILAAALRAYILVIDTHGAWAPFDEETSLYEVELEASGRRHLWGNMTRTAAGIRID
                     AQPVAPLQIDDVVVAIDGIVTSGLSVEQAEQVGILDPNDPNPIRLVSVLRENRPFPTQ
                     LRVQPPTPSFVPNDAYSSIRIHKVPYRQGHVTVIAVFDVPDDLGDELMSALAQARLEQ
                     EPEGILLDLRGNGGGSIEGAKEALGPFLSGANLFPMRRRSGLIEFEQAPVLAPTDLWK
                     GPVAVLVDADTASAAEMIAGAIDAYHRGIVIGSRTFGKGCVQEYLEDDTGMGVLRLST
                     LVYALPNGQPVQRIGIRPVVALDIDRDVDRDVEREDTLPNAMAPWKGPDIRTAQRTRP
                     VPWPTHDGKVGPCDDEVVCQGLRALGARPASVVGRNPGRIRNR"
     gene            complement(7153..8037)
                     /locus_tag="PK710_04245"
     CDS             complement(7153..8037)
                     /locus_tag="PK710_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOT08955.1"
                     /translation="MSLGVVVATLASQGQAFTHVVMQGETLAGIAERYYGRIHYERIL
                     VHANALDACGGVPVVAGMRLEIPAVSHHRVEAGESWQTLARDRLGDARRGEVLAKANG
                     AKSWVPPAEGADIIIPYNLRFVVQQRETAPSIAKRFFSDQESAWMMDRYNGIDGKALQ
                     RGDVVLVPLTDLPLTEEGKRAAKASEAVVRSEGGGAAREAQRVVTAELPQLLAELRGG
                     HYLAASVRANRLLALGELTIPQLADIHRVLTEAYVALDAVGLAAASCQKWRAADPSVT
                     LDEVSMSPKIIAACSKTD"
     gene            complement(8183..9604)
                     /locus_tag="PK710_04250"
     CDS             complement(8183..9604)
                     /locus_tag="PK710_04250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOT08956.1"
                     /translation="MNESRASWTTTLVVGVISLLPRLYAALAFSREPVWDGHYYDYGA
                     RRIAQGLGYSDDLMMGAATLWRPWCHYPVGYSGFLAFLYSIFGNGQFIAPVANALLGA
                     WAVMITHRIGIHWLSPRRAIVAALLCGTSLELILYSPVVMTEVLAMTLPMTAIWIAVV
                     WGKRLSAAVATGAVLGFGTLVQPQTILLAPLLGAVMGGWSLGIRKILWMSFLTTAVAL
                     CVVSPWTIRNCRVMDGCAWVSTNGGWNLAIGAFPRATGRFETLKASDGCAVVTGQVQQ
                     DRCWASLGMSWIKQDFSRWLRLAPLKLGHCFDHASFAVEYLGQADPQLWSEPVRQRWR
                     DWLTTAHRLLLSAAAFGFVAFRVRDAKGFVVETGLRMIVAGFVAYAWSADQPSFWPLA
                     LCIVATGLIPRKSAPALGTAGMALTISFLLFVVTHVIFFGEDRYHVRLIPMMCLLAAA
                     CFRPSRNVDEDSLSRSPTAEAKA"
     gene            complement(9601..11157)
                     /locus_tag="PK710_04255"
     CDS             complement(9601..11157)
                     /locus_tag="PK710_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT08957.1"
                     /translation="MRENHESSTRTVGTPEVRHIIAVGGGRGGVGKSLLTVNLGVYLA
                     QLGREVLIIDADLSGSSLHIQLGLDRAPMADRDAVEHLTVEPVSTSVPGLRLLPSPYD
                     PTVSSPFRPGRKARWMSRLRALPADYVLLHLGAGTSPATLDLFLASDVGLCVTVPDPA
                     AVESTYRFLRSLYLRRLRRVLMKERFKLRLVERVMQTLEPLPSPWGVIDGLSRHDKGL
                     ADLATREMHRIRARLIVNQTRLRTDLELGPTMQTMVHRYLGLTIDYLGHIEQDDQAWL
                     TARRRRPLLVDSPTAKSARNLERIVRRVAALASSLEQRLVEPLPTHEQRTTLYDTLGV
                     PRGASDEEVRRAFKRQRELFSYGSLPLVSLLDEAQVRAELGRIQEAYDTILDPVRRRA
                     YNLSAFPEMSEEEEPAPSRSPITQDQLMLQAELAREIHAETMYSGELLRKVRESQGIG
                     LEEIANSTKISMAHLLAVEEERYDDLPAHVYVRGFVQQLARYLKLDASQVAKTYLRRM
                     RETLAARGQR"
     gene            complement(11527..11907)
                     /locus_tag="PK710_04260"
     CDS             complement(11527..11907)
                     /locus_tag="PK710_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08958.1"
                     /translation="MAASYTIGGASSLAHSPCGCRSCGKRDKNTWHLHRIALGFTSSS
                     EPMALLDWVNQALNDPEVVVVRALCAVAVLVTASCRLDESLSDDSKGMVDNSGSADEC
                     GWWEARFEGFCAPKQVAMPGGVVD"
     gene            11906..13855
                     /locus_tag="PK710_04265"
     CDS             11906..13855
                     /locus_tag="PK710_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08959.1"
                     /translation="MRTLASTYALVGLALFPLACGGASSTTPQTPSSVAVGSEPATAS
                     PPPNLTTVEEPQNIVVIARWKNPKRVFDTLQQWVGIPLPAESGVEDLLRAPGVGAVID
                     MTTPVDVVVALDESSSQTPKAFVSFSMGLRSVQEAREAFSRKREVKDAADGAFRVELG
                     PEWDTLPCIVSPALGSAPARLVCGPRNKDVEILQPFMARGLPVMNLPDRDLVGEVRLA
                     PLQNKYGKMLPQLIQMGSSVASHELGTGDRNLDRAISDTVGELGKELLALSTDLDSLR
                     FETQLEPDAKTLTGEMSLAFQSRTSWLAQRFFDNAEKAGPPPALFWNAPIDSTSVAFD
                     RGSSPSHFASFRRHGGNLLDAALARENLPLADRKEVVALFEKMFEGFPTTITAQGPVL
                     SSKTATTKAGELREAATAALGWYVLGLDENSTRIDAWLQDVSRVYARPTIQRWLRTTS
                     GLDANHLPQVRYGAMATAGLPGLKALQVSIPATLMDASSKNEKPLIYYIMMLPDGNRT
                     WLATGADQASLINQLKAAKTGGASTINSRKDLEALRSARAVGGGFFTMTGIVHQASND
                     IMDVFQGYNSRNSELQQFLAAIPKLPHRGNAPMFITTRVDDGSPARVTVQFKVTRETV
                     EDLRALILTMGGLTGPVPTTPRTNP"
     gene            complement(14134..14442)
                     /locus_tag="PK710_04270"
     CDS             complement(14134..14442)
                     /locus_tag="PK710_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08960.1"
                     /translation="MSTEDQVKAMSRRHMSWPPKANDASRYRGCWVALDNCRYDGSHQ
                     PVEGDVVDSDEDLAELCGRLHEASRGKCIILYCDEDLLIEPHGVRDSDPDFDERLFSQ
                     "
     gene            complement(14547..16277)
                     /locus_tag="PK710_04275"
     CDS             complement(14547..16277)
                     /locus_tag="PK710_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT08961.1"
                     /translation="MECPSCQHPNIDNARFCAKCGTLLPAERPSEDDPMLGMVIKNTF
                     RVVRLLGEGGMGRVYEGEQQMGTTTRKVAIKTLHPHLSQDPQIVERFNRECGTVAELE
                     HPNTIQFFDFGRAPDGSLFIAMEFIDGQSICDIVEKEGPMPPARVEHIFRQVCGSLAE
                     AHKKGIVHRDLKPDNVVLTTRAGEKDFAKVLDFGIAARTEASDAKKEQKLTQQGMVLG
                     TPPYMSPEQFTGKELDGRSDVYSLGVMAYEMLTGKLPFEADTPWEWATKHLTERPYPF
                     EVTAPRATLIPDAMKRAIMRALSKNRDERQSDVMAFFEELSGGGAQQSFVTPGDASIG
                     TSGTAAMAAPPPGILQAPVAVAPVAMAPNYVHPPPTANEMVMPVPAPAYSPAPTAKSG
                     GGGVKGILLAVVGLLFVGGIIGAVLIVRSLSANNDDGPDSLNLGLPSATTMTQIAPLD
                     PTTPSSATPEPTTEQSAAVATTQPTKTAPPSTTTAPAKQLEGEPACQRAIQLASQQAI
                     EAAVAMYRSCQKGGSSVGLAKQRIQSNAKPAAEFAKRRGDCNRARSIANAAKSIGADG
                     GSSAVAASCK"
BASE COUNT         3604 a         5279 c         4196 g         3251 t
ORIGIN      
        1 gtcgctcccg ttattgtgat taccccgccg aagtccatca gctcccatcc gtggggggat
       61 atgtcgatcc ctccctcgag gcgatcctgg cgttacaacc caccttggtc atcggtgcgc
      121 gcggtcccat cggtagaagt ctcgtcgaca agctggaagc acgcgaaatt caatgctttt
      181 ttccgcctac ggagtccgtg gaacagatcc tcgccatgat ctccggcctt gcggaacacc
      241 tcgatacgcc acaagcagcg attcatctca cgacatccat tcgtgagcgg cttcatcatc
      301 tccaactcca ggtccaacca ctatcgagtc cgcggacttt gctcgtcttt gggcaatcac
      361 cgattagcgt ggccggcccc ggcagctttc ccgatgaaat gctgcgattg gcggggtgca
      421 aaaacgcggt cgaacggggg tctcattacc caactctcgg tttcgaaacg gttatcggtc
      481 tcgatccgga tctgatcatc gacgcaacga tggcggcaga acgatcaacc acacccatcc
      541 acgctcaaaa agcgggttgg tcaagcgtac gggcggttcg cgaaggaaat gtcgttcggt
      601 tgggtgacga tcggattttg cgcccgggtc cccggcttgc cgatggggtt gccttgctcg
      661 ctcgacatgc tcataaaggg gtgtcgatac catgagtcag cgacgcggac ccaagtcgcg
      721 cttggatgta cttctcgtgg aacgcggact tgccgaatcg cgcacgcgcg cacaagccct
      781 cgtgatggcg cggctcgtgg aagtcgacgg cgtgaccatt gacaaagccg gaacgacggt
      841 aggagtcgat gcccaggtcg tcgttcggcc ttccggacca acgtacgtat ctcgtggggg
      901 ggaaaaactg gcgggcgcgc tcgtggcctt tgcaccgcat ggattggtcg tcgctgatgc
      961 cgtagccgcg gatttcggcg catctaccgg tggtttcgtc gattgtctgc ttcaacatgg
     1021 agcggcgcgt gttcatgcca tcgacgtcgg acatggattg ctgcaccaac ggcttcgaca
     1081 agaccctcgg gtggtgatgt gggagagaac caatgcacgc tacgtcacgg caaaaacgct
     1141 cggagagagt gccgatttgg tcgtcatcga cgcctcgttc atcggattgt cgaaattgtt
     1201 gccagcagcc cgtgacgtat tgcgtgagca ggggcaattg gttgccttga tcaaaccgca
     1261 attcgaggcg ggacgtcgcc aagtcaccca aggacgcggt gtgatccgcg atgaaaacct
     1321 acgcatgagt gtgattgggc gcatgctcga acacatcgcc cacgacgggt tcacggtcct
     1381 tgcggattcg gcctgtgtga ttgcgggacc caaaggaaac cgcgagcatt tcgtcttcgc
     1441 gcgaaaatga tgaccggcaa acgccgatgg gtgagttgac gttcaaggcg ccgcggcatc
     1501 gactttgatc gtcaccttgt tgattcgatc tccttccgtc aacacatccc atggtccagt
     1561 cgcggttccc acgacggcgt attcaccatt gagatggggt gaatcagcca aagtcacgaa
     1621 aatctggctc gaaccggtat cccgtcctcc tagcgccatt cccacgacac cttgtcgaaa
     1681 cgcgacaggc gtgttttcgc atcgaagtgc ttcgagccca gctccgccat acccatcgcc
     1741 ctgtttgtct ccgaattgca ccacgaatcc gggaaccacg cgatgaaccg ccatatcatc
     1801 gtaaaaccga ttgcgagcga gttgtacgat tcgggtgacc gcgacaggtg caaaggtagg
     1861 gtccagatgc aactgcactt ttcccacttc cgtatcgagt tcgagggtgg ttttgggggc
     1921 caaacgcgcc aattccggag ctggtgacac cgtggtcgag ggggatggtt cacaggtggg
     1981 cggcgcgcct cccaacgact gcaaggcttg cttggcatgc tgacgcaacg tggggttgga
     2041 atcgctgcac aaggatgtga gccaagagcg tgcttgctcg agatgcaacg cgccgcaagc
     2101 gcgtgcgatc gcacccttgg tttcgatggc gtctttcggc cacgaccgcc ccatcgcttg
     2161 caacagcgct tcggcgacct tgggatccgc ttgaaaaggc tccgttttga ccgaaaatcg
     2221 ctcgggactt gctgcaatca acaaagcccc ttcggtcacc acgccaagcg attcatccga
     2281 aagcgccgca accaccaggg gtacgatacc ctttgcttct ggatgtcctg ataacatctt
     2341 gatcgcggcc tgccgaacca ccacggcaac cttggggtcg aggtaggatt tccaaatcgt
     2401 cgcgcgggag ccaaccaact catcgcgatc gatcacggac agtcgtgcga gcgcgcccac
     2461 cgatcctttt tgtgaatcac aagacaacag gcgtggaaag gaagcattcg acccggcacc
     2521 gaggcgagct gccgcacatc gaagcatcga aatgcgacgc accaccgagg ctggagcacc
     2581 agggggcgtg tcgagattcg tcaaggcatc gagaccccga gagcgaaccg ctttttttcc
     2641 cgtcacccga atcgacccca atgctgtcat caacgagccg aaaccggggc cgacaaggga
     2701 ggtcaacacg acagggtcat tgatgggagc caactcatcg atggcgcgta acaactcttt
     2761 ttgtgcggca ggtgaatcga ttttcccaag cgctctggcc gcttccgcac gctccgccgg
     2821 ggaatatcca ctggtggaac gcaacaccgt tccaagcagc tccaccgctt cctctcccag
     2881 cgttcccaga gaacgaagga cgaaaactcg ggcggaaccg tcgtggccaa gccgcttgcg
     2941 acaagcttcg aaaagctgtt cattcacgcg cactggcgca cgcttcaacc gcgtgaaggg
     3001 aaataaagca tcggtcaacg gatcgttcgc ggcatcacca cgcgtggcgc gaagcaaaga
     3061 ggctgctgtt tcctcttcca agcgacgatg ctggctcacc acgtctccaa gtcccatggc
     3121 ggctgcttcc gcgcgttgtt ttggacccga cagccacgac accagacttt gctcggcttg
     3181 caccgacgcg cacttaccga gggccccagc aagagcaacc caaggatcga ggcgtcccgc
     3241 ggctacgggc ggctcgagcc ccagactcac cgaacgggcc accaggcgtg atgtcgtgcg
     3301 ctcgcggtcc cgggcacaaa ttcttccaag accgtatgcc gaccacgcca gcacctccgg
     3361 atcatggtct gaaagagatg ccaacagctt gtcccgaact atttcactct ccgcccgagc
     3421 cagcacacgc acggccgaac gacgcaccct cggatccgcg ttcgtcagat ccgtatccat
     3481 cacttgtccc gccgctcgcg tattctccaa gagaagcagt tcatgcacgc gcgccggctc
     3541 gtcggaacga cgattcgctt cggctgccat cgcaggagca ccttgttttt cagcggtttc
     3601 atccggtccc tttcgatcgg cgggggtaca tccggcgaaa aggagcaatc cactcatcaa
     3661 tcccatggtt aggaaagcgg ggttcattgt gaattcctcc gccacagacg agcaccatcg
     3721 aggcgtgaac acacccattc tctgacaccg tagtatgcgc gctcggggag ccgagccggc
     3781 gtgatggcgg gaatcggcca aacgtccatg tggcaccggc gaaagtcgag ggtggcgcgc
     3841 ggcagatgcc agtcgcaggt aatgatggca agtcttttcc atcctcgatt gtttgccaac
     3901 cgagtgaggt aaaaggcatt ttctgctgta ttttcggata gttgctcaag ctcgatgcaa
     3961 tcgctcggga cacccaattg ctcgagccag cgtttcatgc tcaccgcttc actatggccc
     4021 caccagcgct tacctccggc agcaaccacg acgggagcca caccttgctc gaaggccaac
     4081 cgggcccgtt cgagccgacg ttgcagggcc ggactaggaa gcccagacgg caacacccga
     4141 gcacccaata ccgcgatggc atcacaacga gcagtcacgg cacgaccgta gcggggggga
     4201 accgattggg gaaccacaat ggtgagagaa aggttgaccc gccccggctt gggccttatc
     4261 aacgcgagag tctccacagg gaacagtccg gtctattcag aggagttgac cagaatgcac
     4321 atcaagtttt ggggcgttcg cggtagcatc gccgttcctg gacgggacac cctcgaggtc
     4381 gggggaaata cgagctgtgt cgaagtccgg tgtggcgaca cgctcatcgt gctcgatggt
     4441 ggcacgggca ttcgccttct cgggcaggag ttgctcggaa aactacctgt cgaagcctat
     4501 atttttttca gccacgtgca ttgggatcat atccaggggt ttcctttctt cacgcccgct
     4561 ttcatccctg gaaaccgctt tcatttatat ggcggtaaca acgttaccca caccctcgaa
     4621 gagactctcg cgggacagat ggactacccc agtttcccgg tccatctcac cgacatggcc
     4681 gcccaaatgg actttcacga tcttcgggaa ggtcaggtgg tcaccatcgg ggaaggttcg
     4741 gatctcatcc gaatcagcaa tgcccgaggc aatcacccca acggcgtttt cgcctaccga
     4801 atcgactatc accatcgatc cgtcgtctat tgtaccgaca cggaacacta cgatgtcgtg
     4861 gatgtgaagc ttgcaggcct ctcgaaaaac gccgatgtcc tcatttacga cacgatgtac
     4921 cttcctgagg agtattccgg agaaaaggga ggcggtccca aaacgggatg ggggcatagc
     4981 acctacgaac aaggcatcaa gctggcccaa gaggcaaacg tccaacaact cgtcttgttt
     5041 caccatgatc cattgcacac cgatgccatg gtcgccgaca aggaacgacg cgctcgcgag
     5101 tctttccccg caagcatcgc ggctcgcgaa ggcctggtga tcaaccttta agccgaacaa
     5161 tctgctcggt atcgtcgttt caagctgtgc cttgccccgg caatcttatc cgcaaactcc
     5221 tggttatttt ttccgtgatg ctacgatttc accagccaca tggacgcacg gctttccacc
     5281 tttccgccat ttgcaacctt gctccttgcc gctttttctc tgtccatccc ctcgaattta
     5341 cgcattttac ctcctccttc ctccagcgac cgcgttgcgt cctgcccacc agaagcgtcg
     5401 acacacgccc ccgcgccatc gtctccaccg tacctttctc gcaacctccc cccgccttca
     5461 gacctgcccg tccggctcac ctgcgagcag gccatgagaa tcgcggccca ggcgcggacc
     5521 caactcgccc agcctccaca tcccgtggac ccaatacagt tcgcaaccta taccgccgat
     5581 tggctcgatc cccacggatt gtggtccgcc gccccagatt cacccatcgg ctcagccctc
     5641 caacaaagcg caagcctgct gctttcaagc ttgcagaacc ccaatgaccc ccggggctgc
     5701 cctacggccg tcgcgctcgg aagcgaactc gctcgctggg tccaaaacct gcgaaccgtt
     5761 ttcgacgaaa accttcgtgt accctcgaca ccgtcagacc cacaccgggc cgccacctcc
     5821 gctctattcg aaaacgacag cacccccgcg cttggcctcg ctgccgaact cggacaacga
     5881 ttggctgccc tccaacccat cggcccgttt gcctcccaca tccaaaacgc tatccgaata
     5941 cggatgttcc cccatctttc cgctaacgat tggggtgacg tcatcttggc cgctgccctg
     6001 cgcgcttata tcctcgtcat cgacacccat ggcgcttggg ccccgttcga cgaagaaaca
     6061 tcactgtatg aagtcgaact cgaagcctcc ggacgccgcc acctttgggg aaatatgacc
     6121 cgtactgccg cgggaattcg aatcgatgcg caacccgtgg cccccttgca aatcgacgat
     6181 gtggtggtgg ccatcgacgg aattgtcacc tcaggtctga gtgtcgaaca ggcagaacag
     6241 gtaggaatcc tcgatccaaa cgacccgaac cctatccgcc tcgtatccgt cctgcgcgaa
     6301 aatcgcccct ttcccactca gcttcgcgtg caacccccaa ccccttcttt cgttcccaac
     6361 gacgcctaca gttcgatacg catccacaag gttccttatc gtcagggcca cgtcaccgtc
     6421 atagcggtct tcgacgttcc ggacgatctg ggggacgaat tgatgagcgc gttggcgcaa
     6481 gctcgtctcg agcaagagcc agaaggcatc ttgcttgacc ttcggggcaa tggcggtggt
     6541 tccatcgagg gagcgaaaga agcattgggt ccgtttttgt ccggcgcaaa cctgtttccc
     6601 atgcgcagac gaagtggtct catcgagttc gaacaagcgc ccgttcttgc gcccaccgat
     6661 ctttggaagg gaccggtggc tgtgctcgtc gacgcggata ctgccagtgc cgcggaaatg
     6721 attgctggag ccatcgacgc ttatcaccgc ggcatcgtca tcgggtcacg gacattcgga
     6781 aaaggatgcg tacaagaata tctcgaagac gacacgggaa tgggtgtgct gcgcctgagc
     6841 actcttgtct acgcccttcc caatgggcaa cccgtgcaac ggattggcat tcgacccgtg
     6901 gtggctctcg atatcgatcg ggatgtcgat cgggatgtcg agcgtgaaga cacgctgccc
     6961 aacgccatgg ctccttggaa aggacctgat atccgcacgg ctcaacggac tcggcccgta
     7021 ccgtggccta cccatgacgg aaaggtggga ccctgcgacg acgaggtggt ttgtcagggg
     7081 cttcgcgctt tgggcgcacg ccccgcgtcc gtggtcggcc gcaatccggg ccgcattcgt
     7141 aaccgataga attcaatctg ttttcgagca ggccgcgatg attttggggc tcatcgaaac
     7201 ctcatcgagc gtcaccgaag ggtctgccgc gcgccacttt tgacaggacg cggccgccaa
     7261 tcccaccgca tcgagcgcca catacgcttc ggtgagcaca cggtggatat cggcgagttg
     7321 aggaattgtc agttcgccca aagccagcag ccgattcgca cgaaccgacg cggcgagata
     7381 atggccgccc cgcagttctg cgagaagttg gggcagctcg gcggtaacca cacgctgtgc
     7441 ctctcgtgca gccccccctc cctccgagcg aacgaccgct tccgaggcct tggctgccct
     7501 ctttccctct tccgtcaacg gcaagtccgt caaaggaacc aataccacat cgccgcgctg
     7561 caacgccttg ccatcgatgc cgttatatcg atccatcatc caagcgcttt cctgatccga
     7621 aaaaaatcgc ttggcaatcg atggcgcggt ttctctttgt tgcaccacga aacgaagatt
     7681 gtacggaatg atgatatccg caccttccgc cggtggcacc caggacttcg cgccattcgc
     7741 ctttgccagc acttcgccac gacgcgcatc gcccaatcga tcccgggcca atgtctgcca
     7801 actctcccca gcctccaccc gatgatgaga taccgccggg atttccaaac gcattcctgc
     7861 caccaccggc actcccccac aggcatccaa tgcgttggcg tgaacgagga ttcgctcgta
     7921 atgaatcctg ccgtagtaac gctccgcaat ccccgcaagc gtctcgcctt gcatcaccac
     7981 gtgcgtgaat gcctgacctt gcgaggccaa agtcgccaca acgacaccca aggacatggc
     8041 taaccatcgt gctctcgttc ccatggcgaa aaaaagggta gtcgcgaatc gtcaccaccg
     8101 cacgcccgac ctcgatctcg caccattttc caaaggaatc gacagcaaac ccggcccgaa
     8161 accaccacga tatttgggtc tcttatgcct ttgcctcggc tgttggactt cgcgacagcg
     8221 aatcctcatc gacgtttcgt gaaggtcgga aacaagccgc cgccaacaaa cacatcatcg
     8281 gaatgagccg aacgtgatat cggtcctctc caaaaaaaat cacgtgggtc acaacaaaca
     8341 gaagaaacga gatggtgagg gccatccccg cggttcccaa tgccggcgca ctttttctcg
     8401 gaatcaatcc agtcgctacg atacataacg caaggggcca aaacgaaggt tgatccgcac
     8461 tccaggcata cgccacgaaa ccggcaacga tcattcgtag ccctgtttcc acgacaaaac
     8521 ctttcgcatc gcgaacccga aaagccacga acccaaacgc tgccgcactc aacagcagtc
     8581 gatgggccgt cgtaagccag tctcgccatc gttgccgcac cggttctgac caaagctgcg
     8641 ggtctgcctg gccaagatat tccacggcaa acgaagcatg atcgaaacag tgccccaact
     8701 tgaggggagc caatcgcaac caacgcgaga aatcctgctt gatccagctc atccccaacg
     8761 atgcccaaca ccgatcttgt tgcacctgcc cggtgaccac agcacaacca tccgaagctt
     8821 tgagcgtctc gaatcgaccc gtcgcccgag gaaatgctcc tatggccaag ttccagcccc
     8881 cattggtcga cacccacgca caaccgtcca tcacacgaca gtttcgtatc gtccagggag
     8941 aaaccacaca taacgccacg gccgtcgtga gaaaggacat ccacaaaatt tttcgaatgc
     9001 caagggacca acctcccatc accgccccaa gcaacggagc cagcaagatc gtctgcggct
     9061 ggaccaaggt tccgaacccc aacacggctc ccgttgccac cgctgccgaa agtcgttttc
     9121 cccacacgac cgctatccat atcgccgtca tcggaagcgt catcgcaagc acttcggtca
     9181 tcaccaccgg gctatataga atcaactcca gactcgttcc acagagcaac gccgccacga
     9241 ttgcccttcg tggcgatagc caatgtatcc caatacggtg tgtgatcata accgcccacg
     9301 cccccagcaa ggcattcgca acgggagcaa taaattgacc atttccaaat atggaataca
     9361 aaaatgccaa aaatccacta tacccaacgg gatagtgaca ccatggacgc catagtgtgg
     9421 cagcccccat catgagatca tccgaatacc ccaacccctg tgcgattcga cgggctccgt
     9481 aatcataata atgaccgtcc caaacgggtt cccgactaaa ggcaagcgcc gcgtataagc
     9541 gtggcagcag ggaaatcact cccacgacga gcgttgtcgt ccagcttgct cgactctcat
     9601 tcatcgctgc ccacgcgctg ccagtgtctc gcgcatccga cgaagatacg tttttgcgac
     9661 ctgagaggca tccagcttca aatagcgagc gagttgctgc acgaaccctc gcacatacac
     9721 atgcgccgga agatcgtcat accgttcttc ctcgaccgcc aacaaatggg ccatcgagat
     9781 cttcgtgctg ttcgcaatct cctccaatcc aatcccttgg gattcacgca ccttgcgcaa
     9841 caactctccc gaatacatcg tctcagcgtg aatctcccgt gccaattcag cctgcaacat
     9901 aagctgatct tgcgtgatgg gagaacgtga cggcgcgggc tcttcctcct cactcatctc
     9961 cggaaatgcc gataagttgt aggcacgtcg acgcaccgga tcgagaatcg tgtcatacgc
    10021 ttcttggatc ctccccagtt ctgctcgcac ttgcgcttcg tccaacaagg acaccaacgg
    10081 caagcttccg tacgaaaaca actcccgctg acgcttgaaa gcgcgtcgaa cctcctcatc
    10141 cgatgcgcca cggggaaccc caagcgtgtc gtagagcgtc gtacgctgct catgcgtggg
    10201 caaaggctcc accaaacgct gctcgagaga ggaggctagc gcggccacac gacgaacgat
    10261 ccgctccagg ttccgcgcgc tcttcgcagt cggactgtcc acgagcaatg ggcgtcgacg
    10321 acgcgccgtc aaccaagctt gatcgtcctg ctcgatgtga ccaaggtaat cgatcgtaag
    10381 accaagatac cgatgcacca tcgtttgcat ggtggggcca agctccaggt cggtgcgaag
    10441 gcgggtttga ttgacgatca accgcgcacg aatgcgatgc atctcgcgag ttgccagatc
    10501 cgcaagcccc ttgtcatgtc gcgaaagacc gtcgatgact ccccacggtg atggcaatgg
    10561 ctcgagcgtt tgcatcacac gctcgaccaa tcgcagcttg aagcgctcct tcatcaagac
    10621 tcgtcgcagc cggcgtaaat acaacgaacg aagaaagcga taggttgatt ccacggccgc
    10681 agggtcgggc accgtcacac acaacccgac gtcagaagcc aaaaacaaat ccaaggtggc
    10741 tggcgaagtg cccgctccca agtgcaacag cacataatcc gcaggcaacg cgcggagtcg
    10801 gctcatccaa cgagccttgc gacccggccg aaatggagag gaaaccgtcg gatcatacgg
    10861 cgaaggtagc aaccgcaaac ccggaacgga cgtggatacc ggctcgaccg tcaaatgctc
    10921 cacggcatcc cgatccgcca tcggcgcacg gtccagccca agctggatat gcaaactcga
    10981 cccggataaa tccgcatcga tgatcaatac ttcgcgcccc aattgcgcca agtacacacc
    11041 aagattcacc gtcaacaagc tttttccaac accacctcgc ccccccccaa ccgcgatgat
    11101 gtgacggact tccggtgtac cgactgtccg cgtggatgat tcatggtttt cacgcatcgt
    11161 ccgcctcggc tttgccgcct ttagcaaagg ataacggaac tgccatcacg gaagtgaaca
    11221 gcaacgaaaa ccaatgccga catttcgact ggcacgctcg ttttcataac caaaatcaca
    11281 ccgaaggtcc tcttgcccac tgccgtatac ccccctcgca accgacaccc atcggaaaag
    11341 ccatccccac gacaggagtt ctcccactcc catacgttgc cgctcagatt acatacccaa
    11401 cccgttttca tgcactccgg caacgaccga tcatcatcat ttcaatcaca aatccgcccc
    11461 tgctcagccg ttattggatt catcgcaaac caagagacgt actgcagtcg ggttacttcc
    11521 gtcgcgtcaa tcgaccaccc ccccaggcat cgccacttgt ttcggcgcac aaaacccttc
    11581 gaaacgtgct tcccaccatc cacattcgtc ggccgaccct gaattgtcta ccatcccctt
    11641 cgaatcatcc gagagcgatt catcaagacg gcacgacgcg gtcacaagca cggccactgc
    11701 gcacaacgcg cgaacaacaa ccacttcggg atcgttcaac gcctgattaa cccaatcaag
    11761 caaggccatt ggctcgcttg aactggtgaa cccaagggca attcgatgaa gatgccatgt
    11821 gtttttgtct cgcttgccgc acgatcgaca tccacagggc gaatgcgcaa ggcttgatgc
    11881 tcctccgatg gtatatgatg cggccatgcg aacactcgcc tctacctatg cattggtcgg
    11941 cttggcgctc tttcctttgg cctgtggtgg cgcatcgtcg acaactcccc aaaccccctc
    12001 ttccgtcgcg gtcggctccg aacccgcaac agcaagcccg ccgcccaacc tcacaaccgt
    12061 agaagaaccc caaaatatcg ttgtcattgc gcgctggaaa aaccccaaac gcgttttcga
    12121 tacgttgcaa caatgggtcg gcatccctct tcccgccgaa tcgggcgtgg aggacctgct
    12181 ccgtgctccg ggagtcggcg ccgtcatcga tatgaccaca cccgtggatg tggtcgtggc
    12241 attggatgag agttcttccc aaacacccaa agctttcgtg agcttttcga tgggacttcg
    12301 gtccgtgcaa gaggctcgag aagctttttc ccgcaagcga gaggtcaaag atgccgccga
    12361 tggcgccttc cgggtcgaac tcggacccga atgggatacc cttccttgta tcgtctcgcc
    12421 tgccctcggc tccgctcccg ccagattggt ttgcggtcca cgaaacaaag acgtcgaaat
    12481 cctgcaacca ttcatggctc gaggtctgcc cgtcatgaat ctgcccgacc gggatctggt
    12541 cggcgaggtg cggctcgccc ccctccaaaa taaatacgga aagatgctcc ctcagctcat
    12601 ccaaatgggt tcttccgtcg ccagccacga actgggaaca ggggatcgca acctggaccg
    12661 tgccatctcc gatacggtag gtgagctggg caaagaattg ctcgccctgt cgacggacct
    12721 cgattcactg cggttcgaaa cgcaactcga accagatgcc aaaactctca ccggggaaat
    12781 gagcctggcc ttccaatccc gcacgtcatg gctcgcccaa cgattcttcg ataacgcgga
    12841 aaaagcaggc ccgcctcccg ccttgttctg gaatgctccc atcgacagca cgagcgttgc
    12901 cttcgatcgc ggctccagcc ccagtcactt tgcaagcttt cgtcgtcacg gtggaaacct
    12961 tctcgatgcg gccttggccc gtgaaaacct tcctttggcc gatcgaaaag aagtcgtcgc
    13021 cctttttgaa aaaatgttcg aaggctttcc caccaccata acggcccaag gacccgtgct
    13081 atcgtcgaaa acagccacga caaaagccgg cgaactccga gaggccgcca ccgctgccct
    13141 cggatggtat gtgctgggcc tcgatgaaaa ctccacgcgc atcgacgcct ggttgcagga
    13201 cgtgagccgc gtgtatgccc gcccgaccat ccaacgttgg ctgcgaacta cgtcgggcct
    13261 cgacgccaat catcttcctc aagtgcgcta cggagccatg gcaaccgccg gcttgccagg
    13321 actcaaagcc ctacaggttt ccattcccgc aacccttatg gacgcgagca gcaaaaacga
    13381 aaagcccctg atttattaca tcatgatgtt gcccgatggc aatcgcactt ggctcgccac
    13441 cggcgccgat caagcctcgt tgatcaatca actcaaggca gccaaaacag gaggagccag
    13501 caccatcaac tcccgaaaag acctcgaagc cttgcgttcc gcgcgtgccg tgggaggcgg
    13561 attcttcacc atgacaggaa tcgttcacca agcctccaac gacatcatgg acgtctttca
    13621 aggctacaac tctagaaaca gcgagcttca acagttcctg gccgccatac ccaaactacc
    13681 ccatcgcggt aatgcaccca tgttcatcac gacgagagtc gatgacggct ctcccgctcg
    13741 cgtcaccgtc cagttcaaag tcacccggga aaccgtcgaa gaccttcgag ccctcatcct
    13801 gacgatgggt gggctcaccg gacctgtccc gactaccccc cgcacgaatc cctgacactt
    13861 cctcgcgtaa aaagcggcag tcccccatcc gccgagttga ctgcaactaa ttgaaattac
    13921 tagtttttct ctcgcttccc aagactcccc gggctcccaa tcgcttcatg accacttgcc
    13981 gtcgcggggt ggtgatttcc catcaattca accccagcgc gatcgtgaaa cgataggccc
    14041 atccctagac cggatgcgct tcagccctgc catcaagggc cgtctcggcg tcgaatagga
    14101 tcatccgtcg acagcgcgct cggccatcaa cgactattgt gaaaacagcc gctcatcgaa
    14161 gtccggatcg gagtcacgca cgccgtgtgg ctcgatgaga agatcctcat cgcaatacag
    14221 gatgatgcac ttgccacggc tggcctcatg aaggcgcccg cacaattccg caagatcctc
    14281 atcactatcc accacgtccc cttccacggg ctggtggcta ccatcatatc gacaattgtc
    14341 gagcgcaacc caacatcccc ggtatcggga tgcatcgttg gccttcggcg gccaactcat
    14401 gtgcctgcga ctcatggcct tgacctggtc ttccgtcgac acgtttcgaa gatagtatgg
    14461 cattgtcgtg attccgcgcc accgcctaac accattaccg gcatgccgtc aagttcgctc
    14521 gatgtgggct catggaaaca aatggactac ttgcaacttg ctgccacagc acttgagccg
    14581 ccatcggctc caatcgactt ggcagcattg gcgatgcttc gcgcccggtt gcaatcaccc
    14641 cgtcgtttcg caaactcggc agcaggcttg gcattggatt gaattcgctg cttagccaat
    14701 ccaacactcg aaccaccttt ctgacacgag cggtacatcg cgaccgcagc ctcgatcgct
    14761 tgctggctcg caagctgaat ggctcgttga cacgcgggct ccccttccaa ttgcttggcc
    14821 ggtgccgtcg tcgtcgaagg cggtgctgtc ttggtcggtt gcgtcgtcgc caccgcggcg
    14881 ctttgctctg tcgtgggctc cggtgtcgca gacgaaggtg tcgtcgggtc gagcggagcg
    14941 atctgcgtca ttgtcgtcgc cgagggtagc cccaagttga gcgaatcagg tccgtcatca
    15001 ttgttggccg acaagcttcg aacaatcaga acggcaccaa tgataccacc cacgaagagc
    15061 aatccgacta cggcaaggag gatacctttg acgccaccac caccggattt cgcggtagga
    15121 gctggcgaat atgccggagc tggcacgggc atgaccatct cgttggccgt cggtggcgga
    15181 tgtacgtaat tcggagccat ggcgacgggc gcaaccgcca caggagcttg caaaatcccc
    15241 ggcggcggtg ccgccatggc cgcggttccc gaggtaccga tcgaggcatc ccccggggtc
    15301 acgaaagact gctgcgcccc accgcccgac aattcctcga aaaacgccat cacatccgat
    15361 tgtctctcgt cgcgattttt cgatagcgca cgcatgatgg cgcgtttcat cgcatctgga
    15421 atcagagtgg ccctcggcgc ggtcacctcg aagggataag gacgctccgt cagatgcttc
    15481 gtggcccatt cccatggtgt atcggcttcg aacggcagct tgcccgtgag catctcgtaa
    15541 gccatcaccc caagcgaata cacatcgcta cgcccatcga gttccttgcc cgtgaactgc
    15601 tccggactca tataaggcgg cgtgcccaac accatccctt gttgcgtaag cttctgctct
    15661 tttttggcat ccgaagcttc cgtacgggcg gcgattccga agtcgagcac cttggcgaaa
    15721 tccttttcac ccgctcgtgt ggtgagcacc acgttgtcgg gcttaagatc cctgtgaacg
    15781 attccctttt tgtgggcttc cgccaacgaa ccgcatactt gacggaaaat atgctcgacg
    15841 cgagcgggcg gcatgggtcc ctctttctcc acgatgtcgc agatcgattg gccatcgata
    15901 aactccatcg cgatgaacaa ggaaccgtcc ggcgctcgac caaagtcgaa aaactgaatg
    15961 gtattgggat gctccagttc cgccaccgtc ccgcactcgc ggttgaaccg ctcgacaatc
    16021 tgtgggtcct gcgaaagatg cggatgcaat gtcttgatcg ctaccttccg cgtcgtggtc
    16081 cccatctgct gctcaccctc gtagactcga cccatccccc cttcccccag caaccgaacc
    16141 acacgaaacg tgtttttgat caccatccca agcatgggat cgtcttcgga aggccgctcc
    16201 gcaggcagaa gcgtcccaca ttttgcacaa aatcgagcat tgtcaatgtt cggatgctga
    16261 caagaagggc attccattcg cgtaccacct tcacgttcta caagatcgga tgagcctcgc
    16321 tacaatgctt
//
LOCUS       DAOSOR010000048        16206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_3620_length_16206_cov_4.025076, whole genome shotgun sequence.
ACCESSION   DAOSOR010000048 DAOSOR010000000
VERSION     DAOSOR010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 16206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16206
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3620_length_16206_cov_4.025076"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PK710_04280"
     CDS             complement(<1..369)
                     /locus_tag="PK710_04280"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOT08962.1"
                     /translation="MSFRRRNLTLVGTGLTLPHVDGEAQRYNLATAHLHAAVHADPAL
                     GGVEVRRLDLPFSLNAPCFDQAVVDEVLSTEPDWLGLSCYCWDLAPLLALAARVKALH
                     PPIRIVAGGPSATFEASSLLV"
     gene            complement(401..2761)
                     /locus_tag="PK710_04285"
     CDS             complement(401..2761)
                     /locus_tag="PK710_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08963.1"
                     /translation="MVFNVSSMLLRVGRRFRERPETVVLLAGMILSVWLATEVFRPAL
                     RPVIQTTTAGSDSLIHGLAPSQAVGLNRAHVLTDGVAPRRGDIWNSDLTATFRSSSSI
                     VFDLGASKDIRAAYMVGDGNDEYVFSVSEDGSKYDPLWRAMPESGSGMQPRSSSELHG
                     HGRYVRITARGGDNLYSLGEVQLFETVPNVIPSRVVYRTGLPEGERIRGHFLHFLLAL
                     GITLFATQRKNSWAWKLAATIPSLVALGVLGYEIAGGWPVDQSVVSMARAISAAIAIV
                     ALVREALGRVRWPASRATVLGALAVAGILGVGSFYSLGHLQFPDSEKNRPTFVHTFDM
                     RVYYPVAKYFHELRFDGLYLASVAAYVADAPGATRATMSNVQFRDLKTHRMLKAGEVW
                     DQVLAMETRFTPERWQAFLKDMRYFRLTMGRDYLGSMVDHGANATPVWLAQAHLLFSL
                     TEADELTLVLGGLLDPALLLLAFAAIARAYGWRASLLCMVVFGANDYVMLGTNWAGAT
                     LRHDWLAYLMLAMAALRMKKTWLGGGLLALAAAQRAFPAMALVGLAFPMVGWWIDTLA
                     QTGTRPKRRAWYEANRDILHTWGAAIVVGIVLFLFASLVVSFGAWPEWMRKVTLLDSE
                     PHVNQVSLKAFVGGNDFSQDANVLARWPLFGFVGGAIALGAAYVAWLRREHLDQAAIL
                     GCLLIPILFNPANYYIHFITFLPLLGYPLSREDDPRAIPTWTQVAVWLSMLLLCVAQY
                     WTTKTDDRTVHFETSSALLFFAFGAMLIACHQATKQARSLPQQAAG"
     gene            2875..4122
                     /locus_tag="PK710_04290"
     CDS             2875..4122
                     /locus_tag="PK710_04290"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOT08964.1"
                     /translation="MRHWKAILIALAATAGVAATVHHFVADAQAANARLEHGIPTNPD
                     VLDPPALEGIDLSRITLSKTETTAPAADDRTAHLSIDPDLQRTVQQLFISRGVTEGNV
                     VLMDLRSSRLLVYASITEGARRDTNVEAKAPAASVFKVVTGTALVEKAGLTSDHKQCY
                     LGGLHGFELEDLIEDEQRDKWCATLAGAMGRSLNVIMGRLALRHLDPDSLGSVARAYG
                     FGEPIPFDVPVQPSTFHLPADDDLKFARTAAGFWNTTLSPLQGVVIAATVAQKGLVTR
                     PVLVNAIKDKKGKTIYHAPAAPQTIRQAFSAQVADTVRDMMAETFSNGTAHGDFFDKR
                     GRAYLPNIQLAGKTGTLTSKSQRFYTWLIGFAGATEPEVAFAVLVNNGPKWRTKAPFL
                     CREVLRAYFASHGSPGVSPPALR"
     gene            4468..5901
                     /locus_tag="PK710_04295"
     CDS             4468..5901
                     /locus_tag="PK710_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08965.1"
                     /translation="MADAALPPEQELESEYGSPVATERYVWVANPVSGRVAYVDATTL
                     EVRTVPAGNSPKAIAPVPNPDRDTAIVINELSGDATLLQADDAGNVSTTTFGIANEAN
                     SWAISPSGRWAIAWANADLYPNPNPVQGFQDISVIDVQTEKVSRLSIGYRPIAFSFSK
                     GEERAHAVTQDGISVIDLLLPQGPSVSKLIPTSDDDFETSDTRDVSITPDGSLAFVRQ
                     NGSQDVTVVSLTDGHRIRVQLSGECTDLDLSADGTRAIAAIRDQHEIAILPVPQIATA
                     PEDFEVVSIPNVTVGSVVIAAGAPVAFGFSNAAVEQRMAKIRWDVTPIRVQTMALHSP
                     VLAVFLAENASSAIVVHKDKNSHARAFSALTLDPELPAKIVATQAEVFGVSLTPSGDR
                     AVVAERNDASKIFGAYLVRRDNLQIDRYPLFSPPIAVGTVLGAKRAFVAQQHPEGRLT
                     FIDLDTGLSRTLTGFELAARVIDGSQP"
     gene            5898..7490
                     /locus_tag="PK710_04300"
     CDS             5898..7490
                     /locus_tag="PK710_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08966.1"
                     /translation="MKYSHAIFVGIASITLLSGCGDRPESWDTPAVVRFDKGLENSFA
                     LIDESMHSVHLFSVDESLNLSQVRKNTGKNVVAVMASPQADRLFVLSKGEQPRRKSSD
                     ERPALWVYDGSGSGNLLSKYTLSNPLDSIAVDPEGRFVVLYSSGASGSFVTNRNELLV
                     IDTSLPPGDTNPLPRTIRSFGGTPQRLTFTSPLNLPSGQRRLLIIETDQDLSLLDLDH
                     LDRKEVTVRLSNDYKVVPAGVTYTDGVPQRNDDARLAVRTTKENSVFLLTFVPQANAS
                     AENDFGLTVNIIGLTSTPSDFAFVNTDGGLRLAMLEAGMRRATLVDPDTTTETQVDMP
                     ASFGRISLVTREISDAPAGGSDVAMLWGYEGVAFWSLGKSVGQAYRSIEVLPDIDSSV
                     GNVVSVPAPNQSLKILVPNDAGYGGRRFYVLNLANRTASPLLTSASSLSLTVSADGQR
                     AWFFQRNSREMAVVDLLTMHPQNLVLEQPITSVFDIGRAGGGRAMVALHYQGNMGATV
                     VDAFSPDEASARMHVGLLQGGF"
     gene            7492..8394
                     /locus_tag="PK710_04305"
     CDS             7492..8394
                     /locus_tag="PK710_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08967.1"
                     /translation="MRALRFVCSSVLTLLVSATAMAQEEQEPTTAPSDSATQELPALE
                     PAPMAEPTTPAPSSAASAAPSATVNASHPAGAIDSRQGYAPPPMSDAVDGPSRDMSRD
                     FWQVHLGVRVGWVTSEGYDPFSENDGFSQVSMGATRAFVLSDSISFAPGLLWEYGNAA
                     SKARNAPTELSVHRLGAVAEGRLHLDRDLYVLAKLVPQAVYTGASIEEDSATAVLVRN
                     AWRFGVDATAGAAWNFPRTLGVDDVVPQFWVVGEFGYGWSMNQDLAFSPDVDEDDPRA
                     STTLDLGMLSMRGMMMRISVAMTF"
     gene            8405..9997
                     /locus_tag="PK710_04310"
     CDS             8405..9997
                     /locus_tag="PK710_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08968.1"
                     /translation="MSKLRWYGCLGVGLFMVACGANETDSLRNTAPYPNDTGGSGGYE
                     GPAGGGQAGSGQLPPEQEVESAYESPVATGRFVWIANPLSGRVAFIDAVTLEVSTTEA
                     GNGPSYLAPVPHPSEDVAVVINEVSSDATVLRATASDTIDSISLPIAQQNNAWAISST
                     GRWAIAWTDSRKIAEANETHGFQDIAVLDLSEGAEKATRLSVGYRPVALQFSADEMHA
                     YAVTQDGISVMDLSTGNPVIVGLIPVSDDPFDDPASRDVSITADGMYALVRWENSDRI
                     VVVSLENGMRTEVNLSGTVTDLDLSSDGKKAIAVIREQGEVAVLPIPGIVEAPTAYET
                     VKITNMVVGSATMASEAQVALLYSNASQQDRIAALHYENSPATSDAIKLHAPVLAAFL
                     APTGISSIVLHPPTNSLESSYVSAFSVLHLSPKLPAKIVGMTAPPSAVAISPSGEYGV
                     VAERDDAKKVFGAYLIKMFNQQVDRFELASPPIAVGTLAEAKRAYVAQKHPEGRITFV
                     DLETGLIRTLTGFELGARVVNP"
     gene            10099..10494
                     /locus_tag="PK710_04315"
     CDS             10099..10494
                     /locus_tag="PK710_04315"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOT08969.1"
                     /translation="MLPLMSADSKPPDSIPPSTDERRKAARNVVCVPARVSPREGVDK
                     LSLIEDISLTGARLLTRCEPEMGENLRLSLYLSGTNEAPTIVMARVVRLLPFDGAETL
                     WRRRVAVVFEHPLHGCEQEIHSLAEEEAR"
     gene            10664..13465
                     /gene="topA"
                     /locus_tag="PK710_04320"
     CDS             10664..13465
                     /gene="topA"
                     /locus_tag="PK710_04320"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOT08970.1"
                     /translation="MQKSLVIVESPAKARTIAGFLGDGFIVESSIGHIRDLPRNASEI
                     PSKYKREPWSRLGVNVDNGFQALYVTSPEKRAQVTKLKTLLEKADRLLLATDEDREGE
                     SIAWHLVQVLAPKVPIKRMVFHEITPEAIERALKNPRDIDTQLVNAQEARRILDRLYG
                     YEVSPVLWKKVLPRLSAGRVQSVATRMIVERERARMRFRSASFWDLEVVLQANGGSKT
                     PPFATALVSLDGTRLAQGKDYGEDGKLNRSNVLAMDGAQAKLTAQSMKGGTATVRKVE
                     RKAYQRSPAPPFMTSTLQQEAGRKLRFSAARTMQAAQRLYESGFITYMRTDSVALSDV
                     AVRSAREHIRTLFGDAFLPPKPRKFYNKVKNAQEAHEAIRPAGEVFQRPEDVAQIVGS
                     DEAKLYELIWMRAVASQMADARGERVQMQIEAFADGKKAELAVSGNTITFPGFLRAYV
                     EGNDDPEDSLANMEKPLPELHGGEQLIIESATPRRHETLAPARFTEASLVRDLEAKAV
                     GRPSTFASILATIQDRGYVWKKGTALVPSFVAFSVVALLEQHFPVLVDYEFTARMEDD
                     LDAIAHGTQEMVPWLGKFYFGNQSAGLTASAHQDLAVGLRALVSDNLGEIDARAINSL
                     PIGEGPDGLPIMVRVGRYGPYLAYGDATASIPEDLPPDELTVEKALSLIEVAASERKL
                     GTDPDTGLDVWLRMGRFGAYVQLGETDSEEGKPKRASLFRSMSPDTVTLEQALSLLRL
                     PRIIGVSEGESLEAHHGRYGPYILKGKERRSLETEEQIFTISLSEALALLAQPNRRVR
                     TTASGPLRELGDDPVSGGKITVREGRFGPYVTDGQTNASLRKGDTIDGITLERAAELL
                     VLRREKLATNPKKPRKQGVGPTASKKATAKKAAKKKVSAKKSPTKKAAAKKATAKKTT
                     TKSASAKKTATLKSPTTKA"
     gene            complement(13641..15017)
                     /locus_tag="PK710_04325"
     CDS             complement(13641..15017)
                     /locus_tag="PK710_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT08971.1"
                     /translation="MKLSHRLVGILAAATVGVLGMPSEARAEYPDPGLYVGIYGGGTL
                     KLNDWNLGEAPRSDRDLQPKSAPVVGLRLGYHILPQLIGEVGGAYMPLESTNGEPNTG
                     FKYDVDLFYHLLSSDFSPFLGLGTGAHLTKDDGDLGGDNDIQVHVSLGARGLLTDHIA
                     LRGEVRDYWVDSYSTFGGNNIELTLGLDIYPFGSSAPPPAPADSDGDGVVDADDACPD
                     VPGLAIHKGCPDRDNDSVPDHLDQCPDEPGDPARNGCPAPDKDGDGVLDADDLCPDVP
                     GEVAFQGCLDSDRDGVYDHEDRCPQEHGLKEFKGCPDKDGDKVPDIDDKCPDQPGLPE
                     HNGCVPEEVAKFTGAIKGINFETGSARILPSSFPVLDDAVKVLTEYKSLRLKIDGHTD
                     NQGLADKNQKLSEERAASVKAYLVSKGIDESRLVTAGHGDTKPVEDNATPKGRAANRR
                     IEFSILAQ"
     gene            complement(15363..>16206)
                     /locus_tag="PK710_04330"
     CDS             complement(15363..>16206)
                     /locus_tag="PK710_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08972.1"
                     /translation="VAPVPVLVSRYLVEVFNKAIAPAHGLPPVGSVLIDRANGVVFTM
                     RLGESLLGASKKGNVRSVKARVVGISSRAIDLGLTLPIDTVRRWNEEYSETENDGVFT
                     SLLVEATDARDIGAVVAKAEEFGLMPHDTRARDVGVLVNGVTALLSLVAIVILFMAGA
                     NIAYCFRALLWERRGEIGLYRAVGAARGDIMAWQLSLAAVVGSLYAVGGLSLGWIAMI
                     LADWLAATRLPNFPFKPESFFDCPWWLMMATVVFGALFALAGAAKPAWRAATQDPREG
                     LIGP"
BASE COUNT         3623 a         4743 c         4531 g         3309 t
ORIGIN      
        1 gaccagcaac gaagaagcct caaacgtagc cgaaggaccc ccagctacga tacgaattgg
       61 cggatgcaac gctttgacgc gtgcggcaag cgcgagaagc ggcgccaaat cccagcaata
      121 acaagacaaa cctagccaat ccggttccgt cgacagcacc tcatcgacca ccgcctggtc
      181 gaaacaaggg gcattcaagg aaaacggcaa atcgagccgt cgcacttcga cgccgcccaa
      241 ggcagggtcg gcatgtactg ccgcatgcag gtgagcagta gccaagttgt aacgctgcgc
      301 ctcaccatcc acgtggggta gtgtaagccc ggttcccacc agggtaaggt ttcgacggcg
      361 aaaggacacg tggtattcgt atgggttgcg tggccggcgg tcaacccgcg gcttgctgtg
      421 gaagacttcg tgcttgcttg gtcgcttgat gacacgcgat caacattgcg ccaaaggcga
      481 aaaacagaag cgcggacgat gtttcgaagt gaaccgtccg gtcatcggtt ttggtggtcc
      541 aatactgggc gacgcacagc agcaacatcg acagccaaac ggcaacctga gtccaggtcg
      601 gaatggcgcg cgggtcatcc tcgcgggaaa gcggatagcc aagcagcgga agaaaagtaa
      661 tgaagtggat atagtagttg gcgggattga acaaaattgg aatgagcaaa cagccaagaa
      721 tcgcggcttg atccagatgc tcgcgacgca accaagccac gtaggcggct cccaaagcaa
      781 tcgctccgcc cacgaatcca aacaaaggcc aacgggccag cacgttggca tcttgggaaa
      841 agtcattccc tcccacgaac gctttgaggc tgacttgatt gacgtgcggt tccgagtcca
      901 agagcgtgac ttttctcatc cattcgggcc aggctccgaa cgacaccacc agagaagcaa
      961 agagaaaaag gacgattccg acgacaatgg ccgcgcccca ggtgtgaagg atatcgcgat
     1021 tggcttcgta ccaagcgcgt cgttttggcc gggtgccggt ctgtgctagc gtgtctatcc
     1081 accaaccaac catggggaaa gcgagcccga ccaacgccat ggcaggaaaa gcccgttgcg
     1141 ccgctgccag cgccaacaat cccccaccca accacgtttt tttcattcga agggccgcca
     1201 tcgccagcat caagtacgcg agccaatcat gacgcagggt cgcgcccgcc cagttggtcc
     1261 ccagcatcac gtaatcattg gcgccaaata caaccatgca taatagcgag gcacgccagc
     1321 cataagcccg cgctatcgcc gcgaaggcaa gcaacaaaag cgccgggtcg agtagcccac
     1381 ccaatacgag cgtcaattca tccgcttccg tcagagaaaa caatagatgc gcctgcgcca
     1441 accacaccgg cgtggcgttg gcgccatgat ccaccatgct ccccaaataa tctcgcccca
     1501 tcgtcagacg aaaataccgc atatccttca aaaacgcttg ccaccgctcc ggtgtgaaac
     1561 gcgtttccat cgcaaggacc tgatcccaca cctccccagc cttcaacatt cgatgcgtct
     1621 tcaagtcgcg aaattgtacg ttggacatcg tggcccgggt cgcaccagga gcatccgcca
     1681 cataagcagc cacactcgcc aggtacagcc catcgaaacg aagttcatga aagtacttcg
     1741 ccaccgggta gtagacgcgc atatcgaacg tgtgaacgaa ggtgggacga ttcttctcgc
     1801 tatcggggaa ttgaagatga ccgaggctgt agaacgatcc caccccaaga atacccgcca
     1861 cggcgagggc ccccagaacc gtggcgcgac tcgccggcca acgaactcgc cccagcgctt
     1921 ccctcacgag cgcgacgatg gcaattgccg ccgatatggc tcgcgccatc gacacaacgc
     1981 tttgatctac cggccatccg cccgcgatct cgtaacccaa cacacccaaa gcaaccagcg
     2041 agggaatcgt ggccgcaagt ttccacgccc aagagttttt cctttgcgtt gcaaacagcg
     2101 tgattcctag cgctagaagg aagtgcaaaa aatgccctcg tattcgctct ccctccggta
     2161 gccccgtacg atacacgacc cgagatggaa tcacgttcgg taccgtttca aaaagctgta
     2221 cctcacccaa agagtataaa ttgtctccgc cccgcgccgt gatgcgtaca tagcgcccat
     2281 gcccatgcag ctcgctcgaa gaccgaggct gcatgccgct gcccgactcg ggcatggccc
     2341 gccaaagagg atcgtacttc gaaccatcct ccgacacgga aaacacatac tcatcgttac
     2401 catcgcccac catgtacgcc gctcgaatat ccttcgaagc accgagatcg aaaacgatgg
     2461 acgaactcga tcgaaaggta gccgtaagat cggaattcca tatatcccca cgccgcggcg
     2521 caacgccatc cgtcaacaca tgcgcgcggt tgagccccac ggcttgggaa ggagcgagac
     2581 catgaatcaa actatcgctc ccagccgtcg ttgtctgaat cacgggccgt agggcaggtc
     2641 gaaagacttc ggttgccagc caaacggaaa ggatcattcc cgcgagcaga acaaccgttt
     2701 cgggccgttc gcgaaatcgc cgtccaacac gcagcaacat cgacgaaaca ttgaacacca
     2761 tggcggcacc tataccccgc ggttttcggt acgggaagtc aacatcgaaa cggagaaaac
     2821 gaataggcgt aaacttggcc tccccctcgt tcagcaggta aggacttatg cgacatgcga
     2881 cattggaagg ccattctcat cgctctcgca gccaccgcgg gcgtcgcagc caccgtccac
     2941 cacttcgtgg ccgacgccca agcagccaat gcccggttag aacacggtat ccccaccaat
     3001 cccgacgttc tcgaccctcc ggctctcgag ggtatcgacc tgtcgcgcat cacgttgagc
     3061 aaaaccgaaa ccacagcacc ggctgctgac gatcgcacgg cacatctttc catcgacccg
     3121 gacttgcaac gtacggtgca acaactcttt atcagccgag gtgtcacgga aggcaacgtg
     3181 gtgctcatgg atctgcgctc gtcgcgattg ttggtctacg cttccatcac ggaaggagcg
     3241 cgtcgtgaca ccaatgtgga agccaaagcc ccggcggcaa gcgttttcaa agtcgtcacg
     3301 ggcaccgcgc tcgtagaaaa agcaggccta accagcgatc ataaacagtg ttatctcggc
     3361 ggtctgcacg gtttcgagct ggaagacctg atcgaagacg aacaacgcga caaatggtgc
     3421 gcgacccttg ccggggccat gggacgaagc ctcaacgtga tcatgggacg tctggcgctt
     3481 cgccatctcg accctgattc ccttggcagt gtggcgcgcg cctacggttt cggagaaccg
     3541 attcccttcg atgttcccgt gcaaccaagt accttccact tgcccgcgga cgacgatctg
     3601 aagttcgctc gcactgcggc agggttctgg aataccacct tatcgccttt gcaaggggtg
     3661 gtcatcgcag ccaccgtggc acaaaaaggg ctcgtcacgc gaccggttct cgtcaatgcc
     3721 atcaaagaca agaaaggaaa gacgatctac cacgctccgg cggcccctca aacgatacgc
     3781 caggcttttt cagcgcaggt cgccgatacc gtgcgggata tgatggccga aacgttctcc
     3841 aatgggactg cccacggcga ttttttcgac aaacgtggaa gagcttacct gcccaacatc
     3901 caactcgctg ggaaaaccgg aaccttgacc agcaaatccc aacgatttta cacttggctc
     3961 atcgggttcg ccggtgccac ggaacccgag gtggccttcg ccgtactcgt caacaatggc
     4021 cccaaatgga gaaccaaagc ccccttcctg tgccgagaag tgcttcgcgc ctacttcgcg
     4081 agccacggct cccccggtgt ctcgccccct gccctgcgct aatatcgtat ttggcacatt
     4141 ggctcgcacg acagaattgt cacccccctg cccgcttcta cccccactcc cgcgattcat
     4201 gcctaccatt tccaagttgt attcgtatcg gcacaactgt tgctttgcta cttgcccaca
     4261 ccagcgggtg attggaggtc gcggatgatg acacaaccac gatggctgct cctattcagc
     4321 tccatgatag gaatcgccgg cttcgtcggc gtcttggtcg catgccaggc atccgacgcc
     4381 atggatgagg aaggaagcca aatgccaccc agctaccccg gaaatgaata cccatctctt
     4441 gacggggggg gctccggagg aggcgccatg gcggacgctg cccttccccc agaacaagaa
     4501 ttggaaagcg aatacggctc tcccgttgct acagaacggt acgtatgggt cgccaacccg
     4561 gtcagcggcc gggtcgccta cgtcgacgcc accaccctcg aagtccgcac cgtccccgcc
     4621 ggaaatagcc caaaagccat tgcccccgtt cccaatccgg accgcgacac cgcgatcgtc
     4681 atcaacgaat tatccggtga tgccaccttg ctgcaagccg atgacgccgg caacgtgtcc
     4741 acaaccacct tcggcatcgc caacgaagcc aattcctggg ccatttcccc gtctggccgc
     4801 tgggctatcg cctgggccaa cgcagacttg tacccaaatc caaaccctgt ccaaggcttc
     4861 caagacatct ctgtcataga cgtccaaacc gaaaaagttt caagattatc catcggttat
     4921 cggcccatcg ccttctcgtt ttcgaagggg gaagaacgtg ctcatgcggt aacgcaagac
     4981 ggtatttccg tcattgatct tctgcttccc caagggcctt ccgtttccaa actcatccct
     5041 acctcggacg atgacttcga aacgagtgac acacgcgacg tttctattac gcccgatggt
     5101 tccctggcct tcgttcgcca aaacggttcc caggatgtca cggtcgtttc cctcacggac
     5161 ggtcatcgaa tacgtgttca actttctgga gaatgcacgg acctggactt gagcgcggat
     5221 gggactcgcg cgatagccgc catccgcgac caacacgaaa tcgcaattct tcccgtgccg
     5281 cagatcgcca cagcgccgga ggattttgag gtggtgtcca ttccgaacgt gacggtcggt
     5341 tccgtggtga tcgcggctgg tgctccagtt gcttttgggt tttcgaacgc ggcggtggaa
     5401 cagcggatgg ccaaaattcg atgggatgtc acccccatac gtgtgcaaac catggcctta
     5461 cactcccccg tactcgctgt ttttcttgct gaaaacgcat ccagtgccat cgtcgtccac
     5521 aaagacaaaa acagccatgc gagagcgttc agcgcattga ccctcgaccc ggaactgcct
     5581 gccaaaatcg tggctaccca agccgaggtg ttcggcgtat cgctcacccc atccggagac
     5641 cgagccgtcg tggccgaacg caatgatgcc tcgaaaatat tcggcgcgta tctggttcga
     5701 cgcgacaacc tgcagataga ccgttatccc ttattcagcc cccctatcgc cgtaggcacc
     5761 gtccttggtg caaaacgcgc gttcgttgcg caacaacacc cagaaggccg cttgacattc
     5821 atcgatctgg acacgggatt gtctcgaacc ctgacgggat tcgaacttgc cgcgcgtgtg
     5881 atcgacggga gccaaccatg aaatactctc atgccatctt tgtgggaatc gcgagcataa
     5941 cactcttatc gggctgcggg gaccgtcctg aatcctggga tactcccgct gtcgttcggt
     6001 tcgacaaggg gctcgaaaac tctttcgccc tgatcgatga atcgatgcat tccgtgcatc
     6061 tattttccgt ggacgagtct ctgaatctgt cgcaagtacg caaaaataca ggtaaaaatg
     6121 tggttgcggt catggcctct ccccaggcgg accgcctctt cgttctgagc aaaggtgagc
     6181 agccacggcg aaaaagcagc gatgaacgcc ccgccctttg ggtttatgac ggctcgggtt
     6241 ctggcaacct gctctccaaa tacaccttgt ccaaccctct cgacagcatc gctgtcgatc
     6301 cggaaggccg ctttgtcgtg ctgtactctt cgggagcctc gggctctttc gtgaccaacc
     6361 gcaatgaact cctcgtcatc gatacgagcc tgcctcccgg tgataccaat ccccttcctc
     6421 gcaccatccg gtccttcgga ggtacacccc agcgcctaac gtttacgtcg ccactgaatc
     6481 ttccctcggg tcagcggcgc ctgttgatca tcgaaacgga ccaagacctg tctcttctcg
     6541 atctcgacca tctcgatcgc aaagaggtca cggtcaggtt gagtaatgat tataaagtcg
     6601 ttcccgcggg agtgacctac acggacgggg ttccacagcg caacgatgac gctcgtctcg
     6661 cggtgcgcac caccaaagag aacagcgtgt ttttgctgac attcgttcca caagccaatg
     6721 cttccgcgga aaacgacttt gggctgacgg tgaacatcat tgggttgacg agcaccccgt
     6781 cggatttcgc ttttgtcaat acggacgggg ggttacggct ggccatgctc gaggcgggaa
     6841 tgcgtcgagc gacgctcgtg gatccggaca cgacgacgga aactcaagtt gatatgcccg
     6901 cgtcttttgg gcgtatctcg ctcgtcacgc gcgagattag cgatgctccg gccggtggca
     6961 gtgatgtggc catgctttgg ggctacgaag gggtggcctt ttggtcgctt ggtaagtcgg
     7021 tggggcaggc gtaccgaagc atcgaagtgc ttccggatat tgactcgtcg gtaggtaacg
     7081 tggtgagcgt tccggccccc aatcaatcgc tgaaaatcct tgtaccgaat gatgctggtt
     7141 atggtggccg acgtttttac gtgctgaacc ttgccaatcg cacggcgtcg cccttgctca
     7201 ccagtgcttc ctcgctttcg ttgacggtat cggcggacgg gcagcgggcc tggtttttcc
     7261 agcgtaattc tcgagagatg gcggtggtcg acctgctcac catgcatcct caaaaccttg
     7321 tgctcgaaca gccaatcacc agtgtattcg atattggccg cgcgggaggc ggccgcgcga
     7381 tggtggcttt gcactaccag ggaaacatgg gtgccacggt ggtcgatgca ttttcgccag
     7441 atgaagcctc cgcccgaatg catgtcggct tgcttcaggg agggttttga aatgcgtgcg
     7501 ctcagatttg tctgttcgtc ggtgttgacc cttttggtgt ccgcgactgc catggctcaa
     7561 gaagaacaag agcccacgac ggccccctcg gattcggcga cacaggagct tccagcgctc
     7621 gagccggctc cgatggctga accgaccacc ccggctcctt cctccgcggc ctccgcggca
     7681 ccttccgcca ccgtgaatgc atcacaccct gccggagcca tcgattcacg gcagggttat
     7741 gctccccctc cgatgtcgga tgcggtggat ggtccctctc gggatatgtc ccgggatttt
     7801 tggcaggtac atcttggcgt tcgcgtgggg tgggttacca gcgaaggcta cgatcctttt
     7861 agcgaaaatg atggtttttc gcaggtttcc atgggggcca cccgtgcctt tgtcctgtcc
     7921 gacagtattt ctttcgcgcc tggcctgctg tgggaatacg gaaacgcagc tagcaaagct
     7981 cgaaacgccc ccaccgaatt gtccgtccat cgattgggcg ctgtcgcgga gggccgactt
     8041 caccttgacc gagatttgta cgtgctggcc aaactcgtac cgcaggcggt atacacgggc
     8101 gcttcgatcg aggaggattc cgcgactgcc gtcttggttc gcaatgcctg gcggtttgga
     8161 gtggatgcga cggcgggagc ggcctggaat tttccccgta ccttgggcgt tgatgacgtc
     8221 gtgccgcagt tttgggtcgt gggcgaattc ggttacggat ggtcgatgaa ccaggacctc
     8281 gctttttcac cggatgtgga tgaggacgac ccgcgagcaa gtacgacact cgaccttggc
     8341 atgttgtcga tgcggggcat gatgatgcga atcagtgttg ccatgacgtt ttgaggaggg
     8401 tgagatgtcg aagctacgat ggtacggatg cctgggcgtg ggattgttca tggtagcctg
     8461 tggagcgaac gaaacggatt cgttgcgaaa tacggctcct tatccgaacg atacgggcgg
     8521 ttcgggagga tatgaagggc cggctggggg cggtcaagct ggctccggcc agcttccccc
     8581 cgaacaagaa gttgaaagcg cctacgaatc gccggtggcc actggacgtt tcgtgtggat
     8641 cgccaatccg ttgagtggcc gcgtggcgtt catcgatgcc gtcacgctgg aagtaagtac
     8701 gacggaagcg ggtaatgggc catcgtatct tgcgcccgtg cctcatcctt cggaagacgt
     8761 ggcggtggtc atcaatgagg tttcttcgga tgccacggtt cttcgggcca ccgcttcgga
     8821 tacaattgac agtatttcgc tgccgattgc gcaacaaaac aatgcttggg cgatatcgtc
     8881 cacaggacgt tgggccattg cgtggacgga cagtcgcaag atcgcggaag ccaatgaaac
     8941 ccatggtttc caggacatcg cggtgctcga cttgagcgaa ggagcagaga aggcaacgcg
     9001 gctttccgtg gggtatcgtc cggtggctct gcagttttcc gctgatgaaa tgcatgctta
     9061 cgcggtgacg caagacggca tttcggtgat ggacctgtcc acgggcaatc cggttatcgt
     9121 tggcttgatc cccgtatccg acgatccttt cgacgaccct gcaagccgtg acgtatcgat
     9181 caccgcggat ggtatgtatg cgttggttcg gtgggaaaac agcgatcgga tcgtggtggt
     9241 atccctcgaa aacggtatgc gaacggaggt aaacctgtct ggaacggtaa cggatcttga
     9301 tttatcgtcg gatgggaaaa aggcaattgc ggtgatacgg gagcaaggcg aggtcgcggt
     9361 gcttccgatt cctgggattg tcgaagcgcc gacggcttat gaaacggtga agatcacgaa
     9421 tatggtggtg gggtcggcca cgatggcgtc ggaagcccag gttgcgttgt tgtattcgaa
     9481 tgcgagtcaa caggatcgga tagcggcgct tcattatgag aattcgccag cgacgtccga
     9541 cgcgatcaaa cttcatgctc ccgtgttggc agcctttttg gcgcccacgg gaatcagttc
     9601 gatcgtgctc caccccccca cgaattcgct ggaatcgtcg tatgtgagcg cgttcagcgt
     9661 attgcatctt tcgccgaagc ttccggcaaa aatcgtggga atgacggctc ctcccagtgc
     9721 ggtagcgatc agtccgtctg gcgagtatgg ggtcgttgcg gagcgcgacg atgcgaagaa
     9781 ggtatttggg gcgtatctca tcaagatgtt caatcaacag gtggaccgat ttgagttggc
     9841 gagtccgccg attgcggtag gcacccttgc cgaggccaaa cgggcttatg tggcccagaa
     9901 gcatccggag ggccggatta ctttcgtgga tctggaaacg ggtctgatcc gtactctgac
     9961 gggattcgaa ttgggagcga gggtagtgaa cccttgagaa gcggcgaagc gttgggtcac
    10021 ccgaagcagg acgaattggg cttggcggcg agtccccttc cccgagagtt cacgttgaaa
    10081 cccaatacac atggccctat gcttccgctc atgtcggcgg attcgaaacc acctgactcc
    10141 attcccccat cgacggatga acgacgaaaa gcagcgcgca atgtcgtatg tgtcccagcg
    10201 cgcgtaagcc cgcgggaggg ggtggataag ctatcgctca tcgaggatat ctcgctcacg
    10261 ggagcgcggc tgctgacccg ctgcgaaccc gagatgggcg aaaatctccg tttgagcctc
    10321 tatttatcag gcacgaacga agctcccacg atcgtgatgg cgcgtgtcgt tcggttgttg
    10381 ccctttgatg gtgcggaaac gctctggcgg cgtcgagtgg cggtggtgtt cgagcatcca
    10441 ttgcacggat gcgaacaaga aatacattcc cttgccgaag aagaagcgag atagcacaaa
    10501 aggccactga acggttgacg agtcccatcg aagaaacctc caaaaacccg caccagcgac
    10561 gactttcata ccattgactt gattttcttc ccgcctcacg ccacaagtcc cgagcccgcg
    10621 cgtcccgctt tggggaacct ctcacacaaa accaatatgt agcatgcaga aatcgttggt
    10681 catcgtcgaa tcccccgcca aagcccgcac cattgcaggc tttttggggg acggtttcat
    10741 cgtcgaatca tccattggac atattcgtga tctaccgagg aatgcgagtg agatcccgtc
    10801 gaagtacaaa agggagccct ggtcacgact gggcgtcaat gtcgataacg gctttcaggc
    10861 actgtacgtg acgagtcccg agaagcgggc tcaggtcacc aagctgaaaa cactgctgga
    10921 aaaggcagat cgtttgctac tggcgacgga tgaggatcgg gagggtgaat cgatcgcgtg
    10981 gcatctcgtt caggtgttgg cgcccaaggt tccgatcaag cgaatggtgt tccacgagat
    11041 cacgccggaa gcgatcgagc gggccctgaa aaatccccga gatatcgaca cgcaattggt
    11101 caacgcccag gaggcacgca gaattctcga tcggctttac gggtatgagg tttcgccggt
    11161 gttgtggaag aaagtgcttc ctcggctctc ggccggtcgt gtgcagagcg tggctacccg
    11221 aatgatcgtg gaacgggagc gtgcccgaat gcgctttcgg tctgcctcct tttgggatct
    11281 cgaagtggtc cttcaagcga atggtggatc gaaaacgcca ccgtttgcca cggctctggt
    11341 cagcctggac ggcacgcgtt tggcccaagg caaagactac ggagaagacg gaaaactcaa
    11401 tcgttccaac gtgttagcga tggatggggc gcaagccaag ctcacggcgc aatcgatgaa
    11461 gggagggaca gcgacggttc gcaaggtaga acgcaaagcg taccagcgta gccccgcgcc
    11521 gcctttcatg acctcgacgc tacagcagga agcgggaaga aagcttcggt tctccgcggc
    11581 ccgaacgatg caagccgcgc agcgattgta cgaaagcggc ttcattacgt atatgcgaac
    11641 ggatagcgtt gccttgtccg atgtggcggt gcggtcggct cgagagcata ttcgaacgct
    11701 tttcggcgac gccttccttc cccccaagcc tcgaaaattt tacaacaaag tcaaaaacgc
    11761 gcaggaagct cacgaagcga ttcgaccggc tggtgaggta tttcaacgac cggaggatgt
    11821 cgcccagatc gtcggttcag acgaagccaa actctatgaa ctcatttgga tgcgcgcggt
    11881 ggcttcgcaa atggcggatg cacggggcga acgggtccaa atgcaaatcg aagctttcgc
    11941 cgacggcaaa aaagcagaat tggctgtttc cggcaacacc atcaccttcc ccggctttct
    12001 tcgagcctac gtggagggta acgacgatcc ggaagacagt ctcgccaaca tggaaaaacc
    12061 ccttccagaa ctccacggcg gcgaacagct catcatcgaa agcgcgacac cgcgacgaca
    12121 cgaaaccctg gctcccgccc gattcaccga ggcttccctc gtccgcgatc tcgaagccaa
    12181 agccgtggga agaccctcga cgtttgcttc gatcctcgcc acgatccagg accgcgggta
    12241 tgtgtggaaa aagggcaccg cccttgtccc ttcgttcgtg gctttctccg tcgtcgcctt
    12301 attggaacag cattttcccg tgttggtgga ctacgagttc acggctcgca tggaggacga
    12361 tctcgatgcg attgcccatg gcacgcagga gatggtccct tggcttggca agttctattt
    12421 cggcaatcaa tcggcaggtc ttaccgcttc ggcgcatcaa gatctggccg tcggattgcg
    12481 tgccctcgta tcggataatc taggagaaat cgatgcccgg gcgatcaact ccctgcccat
    12541 tggggaaggt cccgatgggc ttccgatcat ggttcgggtg ggccgatacg gaccctattt
    12601 ggcgtatggt gatgcaacgg caagcatacc ggaggatttg ccgcccgacg agctaacggt
    12661 cgaaaaagcg ctttctctga tcgaggtggc tgcaagcgaa cgaaagcttg gaacggatcc
    12721 ggacaccggg ctcgacgttt ggttacggat gggtcgattt ggtgcttatg tgcaacttgg
    12781 ggagacggat tcggaagagg gcaaaccgaa acgtgcttcc ctatttcgtt ccatgagtcc
    12841 cgatacggtc accttggaac aagctttgtc gctgctccgc ttgccccgca tcattggggt
    12901 ttccgaaggg gagagcctgg aggcgcatca tggtcgatat ggcccctata ttttgaaagg
    12961 caaggagcga cggagcctcg aaacggaaga gcaaatcttt accatttcgc tttcggaggc
    13021 gttggccttg ttggctcaac cgaatcgtcg ggtgcgcacc acggcttcag ggccccttcg
    13081 ggaacttggg gatgacccag tatctggagg gaaaatcacc gtccgagaag ggcgttttgg
    13141 gccttatgtg acggatggtc agaccaatgc ctcgttgcgc aaaggcgata cgattgacgg
    13201 gattacgctc gagcgggcgg cggagttatt ggttcttcga cgagaaaaac tggctacgaa
    13261 tccgaaaaaa ccgcgtaagc agggggttgg ccccacggct tccaagaagg ccaccgcgaa
    13321 aaaagcagcc aaaaagaaag tttcggcgaa aaaatctccg accaagaaag ctgctgccaa
    13381 gaaagccacg gcaaaaaaaa cgacaaccaa atccgcctct gccaaaaaga ccgcaactct
    13441 aaaaagcccc accaccaaag cctgatcgtc gacacgatgg catgatacgg cccttgctat
    13501 cgagccattg cctcgttttc tgaggcccca gcagcacaag tgcgggggcg agctgttggc
    13561 cttggggatt cacccccatc gaacagacca ctagcgataa agacgggtgt tttcgggagg
    13621 gatgggaaac ggagggaagg ttattgggct aggatggaga actcgattcg acggttggcg
    13681 gcgcgcccct tgggagttgc gttatcctcg acgggcttgg tgtcgccgtg gcctgcagta
    13741 acgagcctgc tttcgtcgat gcctttggag acgagataag ctttgacgct ggcggcgcgt
    13801 tcttccgaca gcttttggtt cttatccgct agtccttgat tatccgtatg accatcgatt
    13861 ttcaggcgca ggcttttgta ctccgtgagc actttcacgg catcatcgag aacggggaag
    13921 gacgacggaa ggattctggc gcttcccgtt tcgaagttga tgcctttgat ggccccggtg
    13981 aacttggcaa cttcttcggg gacgcagcca ttgtgctctg gcaggccggg ttgatcgggg
    14041 catttatcat cgatgtcagg gactttgtct ccatccttat ccggacagcc cttgaactct
    14101 ttgaggccgt gctcctgggg gcatcggtct tcgtggtcgt agacgccatc ccggtcggaa
    14161 tcgagacagc cctggaaagc aacctcgccg gggacgtcgg gacaaaggtc atcggcatcc
    14221 aaaacgccat cgccgtcctt atccggggcg gggcagccat tgcgtgccgg atctccgggc
    14281 tcatccgggc attgatccaa gtggtcggga acgctgtcat tatcccgatc gggacaccct
    14341 ttgtggatgg ccaatcccgg gacatccggg caagcatcgt ccgcatccac tacgccgtcg
    14401 ccatcgctat cggcgggtgc cggaggggga gctgaagaac cgaacgggta gatatccaac
    14461 ccgagggtga gttcgatgtt gttgcctcca aaagtagagt aggagtcgac ccaatagtca
    14521 cgcacctccc cgcgcagggc gatatggtcc gtgagaagcc ctcgcgcgcc aagggagaca
    14581 tgcacttgga tgtcgttgtc tccgcccaaa tccccatcat ccttggtcaa gtgagcgccc
    14641 gttccaaggc ctaaaaaggg gctgaaatca ctcgacagca agtgataaaa caggtcaacg
    14701 tcatacttga agccggtgtt gggttcgccg ttggtggatt ccagcggcat gtaggcgcca
    14761 cccacttcac cgatcaactg ggggagaata tggtaaccaa gccgaaggcc aacgaccggc
    14821 gcggacttag gttgcaggtc tctatccgaa cgaggcgcct caccgaggtt ccagtcattg
    14881 agcttgagcg ttccgccacc ataaataccc acgtacaatc ccggatcggg gtattccgcg
    14941 cgggcttcgg acggcatacc tagcactccg actgtggccg ctgccaagat gccgaccaac
    15001 cgatgcgaca atttcatgca agcctcctgc acccttcggt gcggtagaac gcgtttcctt
    15061 ccgactttca aagtacgaat tttcgcggat tcagagcaaa ctcatattcg aacacatacc
    15121 ctacccctgt cctatgtgct cttgcaaccc aacagaacac cgcaagccat catttccaaa
    15181 aaggttttgg cagaagtaca aaaaataatt ataaaacaaa tacttatcca caaaacggca
    15241 ccattctccc ttcctctttc gcaggctcga cccttccaac cgcatcgctt tcaaaccgtc
    15301 atttcgcgag cgctgagtcg agccacgacg atattgttgg ccgctgcgat aagcacggaa
    15361 tgtcaagggc cgataagtcc ttcgcgagga tcttgggttg cggctcgcca tgcgggtttt
    15421 gccgcaccgg ccaaagcaaa tagcgcgcca aaaacgacag ttgccatcat gagccaccac
    15481 gggcaatcaa aaaaactctc aggtttgaaa gggaagttcg gcagacgggt ggctgctagc
    15541 caatccgcga gtatcatggc tatccagccg agcgaaaggc cccctacggc atacaacgaa
    15601 cccaccaccg ccgcgaggga aagctgccaa gccatgatat cgcctcgggc agcccccacc
    15661 gctcggtaaa gtccgatctc tccgcgccgt tcccataata aggctcgaaa acaatacgcg
    15721 atattcgccc ctgccatgaa aagtatcact atcgccacca gagatagcag ggccgtcaca
    15781 ccgttgacga gcacacccac gtctctggct cgcgtatcgt gaggcatgag cccaaactct
    15841 tcggctttgg caacgacagc acctatatcc ctcgcatccg tggcttccac cagcagcgac
    15901 gtgaatacgc cgtcgttttc cgtttccgag tattcttcat tccatcttcg aaccgtgtcg
    15961 atcggcaagg tgagtccaag atcgatcgcg cgtgaagata tgcccaccac gcgggctttg
    16021 acgctgcgaa cgttgccttt tttcgaagct ccgagcagcg attcccccaa tcgcatggtg
    16081 aatacgacac cgttggctct gtcgatcagc acgctcccca cggggggcaa gccatgcgct
    16141 ggggcgatcg ctttgttgaa cacttcgacc aagtagcggc tcaccaaaac gggtactggc
    16201 gctaca
//
LOCUS       DAOSOR010000049        16162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_3635_length_16162_cov_4.509530, whole genome shotgun sequence.
ACCESSION   DAOSOR010000049 DAOSOR010000000
VERSION     DAOSOR010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 16162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16162
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3635_length_16162_cov_4.509530"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2318
                     /locus_tag="PK710_04335"
     CDS             <1..2318
                     /locus_tag="PK710_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08973.1"
                     /translation="LAESFPEVEGIGRQLLSVYQELGWTAEVNALAAQRVKRFPDDLG
                     VLSDWVAVLESMGKVQEADQTVATILKLDPDSPIELDRALRRQDYDKAISELERLGKL
                     RPDRNDIATRLRSVLLRAGKKRETIEDLEKALRETPRDGSTRLALADARLAIGQHAAL
                     RMAVADAIEAGADTSELEGAIELLEGRTELEPYRMDGRKVIEEFESTGGKLDAAAVRV
                     LDYGVTWVVRDGSSRLLEHEIVRVQSQDAIAALTEQRVPRGLVLRLRVIKKDGQILEP
                     ELGIDKPTLTMPHVEIGDYIETEWITSLRGDGHGAYLGPHWYFREQDIGYWRSEFVVI
                     SPTAQPLTIETNGPVPEPTVETRGPIEIRRWRVDKSPAAVIEPGAVPIRELLPNIRIG
                     WGISLERRLRNLADSFEDQSIPDPRLRGIAQRIVKGIPKNQTHERARRVYRWILANIE
                     QGEERDGRRIVTGRSGDLGFCFLYLARLLQIPTDIAVVKNGLEQPPRGPISEAESYQN
                     FVLRIQTDKGDSWLTVRDRFTPFAYLPSALRGQPGYLLREGTPAIKTSAAGAFDGVIY
                     EGQGQLRPTGAAAISLSRRFVGKYAIGVRYSIEQVSEAELPDVVESQLLSKDLPGASL
                     VSVQVLDRDELDKPLTLQMQTEVPDFARRSQTGLTISPPFTVAVGALATLPSRQTTLL
                     LGESSRIEIRVRIKLPPGAKVTTPLAPVQLRDEDRIVVVRDRVEGEELVFDRWIDLPA
                     ARIHPDQYGAFLRFARAADEATQRDIRIEIK"
     gene            complement(2392..3516)
                     /locus_tag="PK710_04340"
     CDS             complement(2392..3516)
                     /locus_tag="PK710_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08974.1"
                     /translation="MRARCLLASASIAAALIASQSEANAEPMDPALERLVLNPGCHML
                     PSPSGAASNYGTWNPAGGAGSDGRCRPDNVAFKRLINQYGAALAPTALHNARTTGYGG
                     FELALEGSFTSIDDSSDYMQNGTRGTIDPSTNRRSIRNNGPDPLAQVYYLKARKGFPF
                     GLEITGLIGTMSHTSFTLIGSDVRLSVLEGFRQGFFGVLPDLAVGGGVRTITGTPQFQ
                     LTTAAVDAQISKPFPIVDSSILTPYVGYQYLWIFGDSGLIDTTPNTNPLGICGYQGPN
                     TPGTPGTPVPTPSGDKPYDGQPVCDGGTPLDFNNTIVFEQVRLQRQRIIVGVNYRFEM
                     LSIGLQYLTDLASPSSANNDSDLKGIPTQYTMSAHLGAIF"
     gene            3727..4185
                     /locus_tag="PK710_04345"
     CDS             3727..4185
                     /locus_tag="PK710_04345"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HOT08975.1"
                     /translation="MLVEPEIPQNTGNIARLCAATQCPLHLVGTLGFQIDERAVRRAG
                     LDYWHLVELHRHLDIAHFRNSFPSCRCILLSARAHRSYLDANFQPGDAIVFGRESVGL
                     PDEWLLDHPDDVYGIPTLGAVRSLNLANAAGIIVYEALRQIGALHHTFLG"
     gene            5258..5331
                     /locus_tag="PK710_04350"
     tRNA            5258..5331
                     /locus_tag="PK710_04350"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5292..5294,aa:Arg,seq:ccg)
     gene            complement(5564..7270)
                     /locus_tag="PK710_04355"
     CDS             complement(5564..7270)
                     /locus_tag="PK710_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08976.1"
                     /translation="MFRRFRLAALTSATVFALGAVSITAVAAPKDAAAKKIDQDTMNN
                     DFVMADFRKAEANLKKALDTCGDSGCSPAVKAQILVNLGIVQSSLGNDTEAEETFVQA
                     IRLHGAVVPDADFVTPEIQKAFDAAKARASSGDTGSVTVDVPPTSDGEVGHEPVSEQM
                     VNTPVPIWVSVPEGMPVGKIIVRYKPFGGSWKKLNLTKKRNGWGGNIDCNDVTTTGAL
                     KYYISVLDNAGDPITSLGSLNKPFETKIKNQLDGDPPRLPNEPPPARCAAKEDCPPGL
                     PGCPAVGARGDKGWGSSCEATRECKEGLICLNGMCEEGEEDTSSGGSSANYAKNWVGL
                     FVSMDLAYVTGDDVCGRDSQANKGYACFTTGGDGGNPYEDAGTHYHGEPIPSGNGNAI
                     AGGLAPATIRFMASFDRVILDNIMVGGRAGFAIRGGPQPDGGKAFLPLHLEARGSYWF
                     GQDPFSRVGFRPYAFLGGGMAQVDTKVDVSVREAQTCPATGCTVSSDPKDPNSVIQRN
                     PQTQKVEAWRKAGQGFVGLGGGVMYALSANSGVVGELKIAYMLPTPNIVIAPTIGYAM
                     GF"
     gene            complement(7325..8479)
                     /locus_tag="PK710_04360"
     CDS             complement(7325..8479)
                     /locus_tag="PK710_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT08977.1"
                     /translation="MTTRWMKMGNLGRRSAYPLVGLIVLLATVLLAPACTTSSVMRGK
                     IEGLQKIADQAERNGAIRCAPRELAMAKSHLRFALLELDQGFVSRATRHLEIAEPNTH
                     AAYSLSPPQVCAERDFVMEKPEPKPGDRDGDGYLDPEDQCPDEPETWNGYQDEDGCPD
                     DPDTDGDGIPDSRDMCVLEPEDMDGYLDEDGCPDLDNDGDGIVDIVDKCPNEPEDPDG
                     FEDADGCPDLDNDGDGVPDLEDICPNEAGPAGGDRPGCPKKPSLAIVTDKEIRILQQI
                     HFEYNKANIRPESYPVVDAVAEIMKQNPKIRIEVQGHTDNRGNKRYNQELSEKRAGAV
                     MQALIARGIERDRLRSQGYGMDRPLVPNDTDQNRALNRRVQFIRIESETH"
     gene            complement(8476..8853)
                     /locus_tag="PK710_04365"
     CDS             complement(8476..8853)
                     /locus_tag="PK710_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HOT08978.1"
                     /translation="METPNPAVDRPGLPGGETLVIMQRVRSAARAAAVACALLATSGC
                     GGVLYAAYAGGAAAKLEQAKEVGAEERAPYEYYFALEHMTKAQEEASQGDYGDAADLA
                     QVAEEYADKAIRLAREAHRGAGR"
     gene            9089..9559
                     /locus_tag="PK710_04370"
     CDS             9089..9559
                     /locus_tag="PK710_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOT08979.1"
                     /translation="MGSHIRQEQLARAEIFSGLTSEGLRLLADIAIEEEFPSETTIFS
                     HGAPGDKLYLILEGKVRISRTVPGMGEEALAVLQQGDVFGEMALLDEAPRSADAKAHE
                     PCLLLSITKERFEDLLFLHKDLAYEVLWNVIRTLIRRLRATNEKLAFLSISGRF"
     gene            9609..10244
                     /gene="lexA"
                     /locus_tag="PK710_04375"
     CDS             9609..10244
                     /gene="lexA"
                     /locus_tag="PK710_04375"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOT08980.1"
                     /translation="MQGLTHRQQQVLDFIRESISERGYPPTLREIGAHMGIRSTNGVN
                     DHLRALERKGYLTREDMKSRALRPKDRDREAIIGDDDNVIEIPILGRVAAGAPILADE
                     HVIDSVHVTPRMLRGAKDVFGLRTSGDSMIDAGILNGDYIFVRRQNTANRGEIVVALI
                     NDEATVKYFFPEKDYIRFQPANAQMAPILVRASDFRSTMILGVVVGVFRKL"
     gene            complement(10265..11161)
                     /locus_tag="PK710_04380"
     CDS             complement(10265..11161)
                     /locus_tag="PK710_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08981.1"
                     /translation="MARPVRRRKLKTVPDRGDPLAAVREFGRRATRTGLIVGFTVATI
                     THGFFGGRALAAPTEMRWWAESVQRVVHEYLWSNYDIDVVKPPPEPVVDEAKNDAAKE
                     KEEANPVTPAPRAAVEPASPPPAAAQAGKLLTAPEQPDAPLDLTGDGFVTGEADSFVG
                     GVTAAAGTGTTPTYNTAASPTGKPGATGSSRKPPPAPSQLSGPDRSRGATIAQGTNWS
                     SCPFPPEADVDQVDYAVVTLVVTVRADGTARSVQVVSDPGHGFGRAARMCALSKRYQP
                     AWDRDGNAIMATTPPIRVTFTR"
     gene            complement(11168..11611)
                     /locus_tag="PK710_04385"
     CDS             complement(11168..11611)
                     /locus_tag="PK710_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOT08982.1"
                     /translation="MISGSTDDTDGITGINIVPLVDITLVLLIIMMVTAKIIVSQSVP
                     MDLPKASSGQNVQMIFAVQLYKNGDVFVDQKKLPGDEAILGLAREARSKNEDLRAVIQ
                     AEQAVTHGRVIGVLELLKTAGISKIAFGVTPVDKVDESRKDGEGK"
     gene            complement(11608..12315)
                     /locus_tag="PK710_04390"
     CDS             complement(11608..12315)
                     /locus_tag="PK710_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOT08983.1"
                     /translation="MNIVEWLKRIMVGFGAAWVMWLLIILSIVSVAVMLERAWFFLSI
                     RDNLANLSKRLRELLRKGDIEGAISDMGKSPSAEAAVVVAGLMEADRGPKAAEEAMRG
                     AAALQRIRLEKRLAILGTLGNNAPFIGLFGTVIGVVMAFEELGKHSQAPAGEAAAVMA
                     PQTVMWAIAEALVATAVGLAVAIPAVMAFNFFQRLIKSTLANTETLSGVLLSYLHAVD
                     AGTAAPPGSEEQRGESS"
     gene            complement(12349..13467)
                     /locus_tag="PK710_04395"
     CDS             complement(12349..13467)
                     /locus_tag="PK710_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HOT08984.1"
                     /translation="MSFERSRPSATIRSTPSDFIVEELALYEPSGSGGHVFLRLRKTG
                     WSTAACARRIAQHLGIANDAIGHAGLKDRHAVTEQTLSFPWPESAALPSMSELASEGI
                     EALQLTRHPHKLRVGHLAGNRFTLVLRNIVGEQAALQQALETLKTQGVPNAFGPQRFG
                     RDGNNPEQTLQWLRGQSRGPKDKRIRRLLLSSVQSLLFDRLLEKRVQQGTWNTVVAGD
                     LVKTTAHGGLFLTEDPETDARRALVGEVSATGPIFGPRMRWPQGEPAQWEKEVLESSL
                     GGEKALEALGKAGAGSRRALRVLPSDLDYRFFEDEGNSCLWLTMVLPKGAYATTVLGS
                     VFVLEDGSEAAKVASGRGTVADSPPNEEMLDDTGPSSR"
     gene            complement(13489..14298)
                     /locus_tag="PK710_04400"
     CDS             complement(13489..14298)
                     /locus_tag="PK710_04400"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT08985.1"
                     /translation="MASLDRRLYRRYLGFFEGMMAFLFRRGRSLGFVGLAGLLVAALP
                     TPASARRPVCHLLNMVLVDGSFCIDRYEAVTQEKKGNRWEFHSPFESVANKQVRAVSR
                     PGRYPQAYISRNEAAAACERSNKRLCTETEWKTACKGKVPTLYPYGKEHKPGHCNDNG
                     LSPLNHYYGVKGGPPLSAYGWSPMNDPKLNQLRGTLAQTGAFSRCRNAWGVYDMVGNL
                     HEWVQDTSGVFLGGYYLDTKINGEGCNYKTTAHNAAYHDYSTGFRCCADPR"
     gene            complement(14348..15745)
                     /locus_tag="PK710_04405"
     CDS             complement(14348..15745)
                     /locus_tag="PK710_04405"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOT08986.1"
                     /translation="MKGGVKKRAVVVCLLWWICLMVVSNAAIAEDREAAQRHFRAGVT
                     LYHEQNFEAALVEFEASYSELASAAALQNIALCQTKLFRYVEAIDTFETLRERFGESL
                     TKKDADSVQQALRNLSQLVGTLTLQVEPRTATVVVDGKRLSSEELARPIRLSSGPHRL
                     EITAPNYMGVTQTIAVAGGESRLVPIELKQAVGLLAVDVDDDDAVVVVDGKPLGRGGW
                     EGEVQAGEHLLEIVKAGHATVATRVRVRADDVVELRLLVGPEQEGANTSLGYDPATLP
                     YRKGRTWLDQDRGWYGSVMASVLAVLRSPDGFEAEDGADVTGGHFGLRAGYRLGYLGI
                     EGAYDTGSQSVSGTWRGMPQTYDLSGHRAGGNLRLLLGGRSIRFTAALGAGVVWHRLE
                     LAGREFKGTNSYVALDMGPQINVGKVLLDFVLQGYGEGANGMRDGEERIYTKRTVLPQ
                     VGLGLRVGFSQWGTW"
BASE COUNT         3468 a         4944 c         4319 g         3431 t
ORIGIN      
        1 acttggcaga atccttcccc gaggtcgaag gcatcggtcg tcaactcctg tccgtgtatc
       61 aagaactcgg atggacagcg gaagtcaatg cgctcgcggc acaacgagtg aagcgttttc
      121 ccgatgatct gggcgtgttg agcgattggg tcgccgtgct cgagtccatg ggaaaagtcc
      181 aagaggcaga ccaaaccgtc gctaccatcc tgaaactcga ccccgattcc ccgatcgaac
      241 tcgatcgtgc cctgcgacga caagactacg acaaagccat atcggagttg gagcggttag
      301 gtaaactccg acccgaccga aacgatatcg ccacccgtct ccgctccgtt ctgctgcgtg
      361 cggggaagaa acgcgaaacg atcgaggact tggaaaaagc gctacgggaa acgccacgag
      421 atggttctac ccgcctagcg ctcgcagatg cccgtcttgc catcggtcaa catgccgcgt
      481 tgcgcatggc cgtcgccgat gccatcgagg caggcgcgga tacctcggaa ctcgaaggcg
      541 ccatcgaatt gctcgaagga cgcaccgaac tagagcccta tcgcatggat ggccggaagg
      601 tcatcgagga attcgaaagc acgggaggca aactggatgc cgcggccgtt cgcgtactcg
      661 attatggagt cacttgggtg gtgcgcgatg gctcctctcg acttctcgaa cacgaaatcg
      721 ttcgtgttca atcccaagac gccatcgcag cgttgacaga acaacgagtc ccccgaggat
      781 tggtattgcg tcttcgtgtc atcaaaaaag atggccaaat cctcgagccg gaattgggaa
      841 tcgacaagcc aacgttgacg atgccccatg tggagatcgg cgattatatc gaaaccgaat
      901 ggatcacatc gcttcgcggc gatggccacg gcgcttatct tggtccccat tggtattttc
      961 gtgagcaaga cattggctat tggcgcagtg agttcgtggt gatttctccc actgctcagc
     1021 cgttgaccat cgagaccaac ggtcccgtgc ccgagccgac ggtcgaaacg cgggggccca
     1081 tcgaaattcg acgatggcgc gtggacaaaa gcccggctgc cgtaatcgaa ccaggagccg
     1141 tgcccattcg agaattgctg cccaatattc gtatcggttg gggcatctcc ttggagcgcc
     1201 ggttacgtaa cctagcggat tcattcgagg atcaatctat ccccgaccct cgcttgcgcg
     1261 gcatcgcaca acgaatcgtc aaaggtatcc ccaaaaacca aacccacgaa cgagcgcgtc
     1321 gcgtctatcg gtggattctc gcgaatatcg aacagggtga ggagcgggat ggcagacgca
     1381 tcgttacggg acgaagtggc gatctcgggt tctgcttttt gtatctcgct cggctcttgc
     1441 agattcccac ggatatcgcc gtggtgaaaa acggtctcga acagcctccc cggggaccca
     1501 tcagtgaagc tgaatcgtac caaaacttcg ttctccgaat tcaaaccgat aaaggcgact
     1561 cgtggttgac cgtgcgcgat cggttcactc ctttcgctta ccttccctcc gcgttgcgcg
     1621 gtcaaccggg ttacttgctt cgcgaaggaa caccagcgat caaaacttcc gccgcgggcg
     1681 ctttcgatgg cgtgatctat gagggacagg gccagcttcg ccccaccggc gccgcggcca
     1741 tttccctttc tcgacgtttc gtcggcaagt acgcgatcgg cgttcgatac agcatcgaac
     1801 aggtgtcgga ggcagaactt cctgatgtcg tggaaagcca actgctgtcg aaagaccttc
     1861 caggcgctag tttggtcagt gtgcaagtgc tcgaccgcga tgaattggac aagcccctta
     1921 cgttgcaaat gcaaacggaa gtccccgatt tcgcacggcg atcgcaaaca gggctgacga
     1981 tttctcctcc gttcaccgtc gcagttggtg ctttggcaac gttgccttcc cgccaaacga
     2041 cccttttgct cggcgagtct tcgcgaatcg agatcagagt tcgcatcaag ctgcccccgg
     2101 gagcaaaggt aacgacaccc ttggcgccag tgcaactacg agatgaggat cgaattgtcg
     2161 tggtgcgcga tcgtgtggaa ggagaggaac tcgtattcga tcgatggatc gatttgcccg
     2221 cggctcgaat tcatcccgat caatatgggg cgttcttgcg gttcgcacgc gccgccgacg
     2281 aagctacgca acgcgacatc cgtatagaaa tcaaatgatc gcgttgcgcg agcacccgat
     2341 gccaccatca ttcatgacag catcgttgga catggcttca cgctagccgc gttagaatat
     2401 ggctccgaga tgagccgaca tcgtgtactg cgtgggaata cctttcaaat cggagtcatt
     2461 gttggccgat gaggggctag cgagatccgt caggtattgc agaccgatgg aaagcatttc
     2521 gaagcggtag ttgacgccca cgatgatgcg ttgccgttgt aggcgaactt gctcgaacac
     2581 gatggtgttg ttgaagtcaa gcggcgttcc accatcacat acgggctgcc catcgtaagg
     2641 tttgtcaccc gatggagtcg gtacgggcgt gcctggcgtt cccggagtgt tgggcccctg
     2701 atagccgcat ataccgagag gatttgtatt cggcgtggtg tcgatcagac cggaatcccc
     2761 gaagatccac aggtattgat agccgacgta cggcgtgagg atggaactat ccacgatggg
     2821 aaatggtttt gaaatttgtg cgtcgacagc cgcggtggtc agttgaaact ggggcgttcc
     2881 cgtaatcgtt cgcacgccgc cgcccaccgc gaggtcaggc aacacaccaa aaaaaccttg
     2941 tcgaaatcct tcgagcacgg acaaccggac atccgaacca atgagggtaa aactcgtatg
     3001 cgacatggtc ccgatcaagc cggtgatctc gagaccaaag gggaatccct tccgcgcttt
     3061 gagatagtaa acctgcgcga gaggatcggg gccgttgttg cggatgcttc gacgattggt
     3121 gctcgggtca atggtgcccc gtgttccgtt ttgcatataa tcggaactgt cgtcgatcga
     3181 cgtaaaggag ccttcgagag cgagttcgaa gcctccgtag ccggtagtcc ttgcattgtg
     3241 cagggcggtt ggggctaggg ctgcgccgta ttgattgatg agacgcttga aagcgacgtt
     3301 atcgggtcga catcgtccat cgctgccggc acctcccgcc ggattccagg tcccataatt
     3361 gctggccgct cccgaagggc tgggaagcat atgacagcct ggattgagga cgagccgttc
     3421 caaagccggg tccatgggct cggcgttggc ttccgattgg ctggcgatca acgcagcggc
     3481 gatggacgcc gacgcgagca ggcatcgtgc acgcattcga ttctccggtg cgggaggcta
     3541 ctttccgcga ggcaagcgcg tcaagcctag gatgcatctt aggctgtttc gtgggttttt
     3601 agcgatccag ggtggcgttg cgccgggcaa ttgtcctaca acgcccccgc attggaccat
     3661 acgcacccca aatcatccga ttcgctcaga ctacgggccc ttccgctcga accacccctg
     3721 cacgtggtgc tggtcgagcc agaaataccg caaaatacag gcaatattgc cagattatgt
     3781 gccgccaccc aatgccccct gcacctcgtc ggcaccctcg gcttccaaat cgatgaacgc
     3841 gccgtacgcc gagcaggact cgactactgg cacctggtcg aattgcatcg ccacctcgat
     3901 atcgcccact tccgaaactc attcccctca tgccgctgca tcctgctcag cgcccgcgct
     3961 caccgaagct acctggatgc caacttccaa cccggtgatg ccatcgtctt cggacgtgaa
     4021 agcgttggct tgcctgatga atggctcctc gaccatccag atgatgtcta tggcattccc
     4081 accctcggcg ctgtgcgctc cctgaatctc gcgaacgccg cgggaatcat agtctatgaa
     4141 gccctgcgtc agatcggcgc cttgcatcac accttcctcg gatgacccca aaacaagggg
     4201 ccacaaaata ggtgaacgag gcataaccaa tgggtttgcc tcgttcacgg gtcgttgggg
     4261 aggttgcgag atgtggcttc cgaacggtaa gatgctcctt tcgcgatggg ttgccttggg
     4321 tggggaagag tctgtgtccc gcccgtcgta ggccataacc acccaagcga agatccattc
     4381 ccgaaggttt gatttttttg ttcaatggat tgcacgggcg aacaaggcga tgggggaagc
     4441 acggcatcac ccaactctcc ttattgtcgc ccttgggcct tcggaaccgg accacgataa
     4501 cgatggcagc ggcattggcc gtaccatcgc cgccgaccat gaacctatgg ggagtgaatg
     4561 gattccacgg atgcctagat atccgtatag ggatatatgt tgttcgcgat tgacaccctc
     4621 cgaaaaccga atttttggag ggataggtta tcgtgagggt aggtatccct tttccatggc
     4681 cgtgggactg atacagtcag taaaatcaat acaaacaagt tgaaaacaaa cggtagcatc
     4741 gcgcaaaaca tcgatacggt cgatggttcg aagctggaaa accgatccca tggtcgtggc
     4801 gtaactatac ctattcgtat aagatggaat ggcaaggatc tcctgccctt gatgcaagaa
     4861 aatggtttat gcgatttcta gaagcttggg ctgctagtcc aaaccacggg gacgccaaaa
     4921 ccgtttctgt ggctcgaggg gacgacaaag cgaacggggc aaagtcaagc gacttgcccc
     4981 gttcatcggt cgttgaggag gttgcgagat gtggcttttt tcggtaaggg ctcctttcgc
     5041 aaagaggtga gtcacggacg aaaaggcttt tctatcccgt ccgtatggta tcaaacgcac
     5101 cacccccgaa ttcattccca cgaatcgaca aaaatgaatc agtgccggtt tgcagaaaac
     5161 gtgcgaaacg atcgccttgg atttgctaca gcccgcgatt cacccttgcg aaggtttcat
     5221 ccaccaggta ggttcgggcc tcgccatcca tggcactgcg ccactagctc agttggatag
     5281 agcgtcggtc tccggaaccg aaggtcgcgc gttcgaatcg cgcgtggcgc gcgatattgt
     5341 cgacgatacg ggcgtttgct cttcctgggt ggatggaaga tccacacccc cgtctaccca
     5401 cccctagccg acaatcgcga tcgcgagata ctcccagaca tttcggttac taggaatatg
     5461 gtctgccaat taccgttacg acgctgggaa agcgatgcga accagcatgc ctttcgtggg
     5521 ggagatgacg gggatgggcg cgaggggaaa ggggagaaag cctttagaat cccatggcat
     5581 agccgatggt gggcgcgatg acgatgttgg gggtgggaag catataggcg atttttagtt
     5641 ccccaacgac cccactattg gcggagaggg catacatgac cccgccgccg agacccacga
     5701 atccctgacc tgcttttcgc catgcttcga ctttttgggt ttgcgggttg cgctggatga
     5761 ccgagttcgg atctttgggg tcggaactga ccgtacaccc agtcgctggg catgtttggg
     5821 cttcccggac ggatacatcg actttggtgt ctacttgggc catgccgcca cccaagaaag
     5881 cgtaggggcg gaatccgacc cggctgaagg gatcttggcc gaaccaatag gagccgcgcg
     5941 cttccaaatg aagcggaaga aatgctttgc cgccatcagg ctgaggtcca ccacggatgg
     6001 caaatcctgc acggccaccc accatgatat tgtcaaggat gactcggtcg aagctcgcca
     6061 tgaatcgtat ggtggccggt gcaagaccgc cagcaatggc attgccattg ccggacggaa
     6121 tgggttcacc atggtaatgc gtgccagcat cttcgtaagg gttgccccca tccccacccg
     6181 tggtgaaaca ggcgtatcct ttgttggcct ggctatcacg gccgcagaca tcgtcgcccg
     6241 taacataggc gagatccatg gacacgaata gaccaaccca gtttttcgca taattggccg
     6301 atgaaccccc actgctggtg tcttcttcgc cctcttcaca cataccattg aggcaaatga
     6361 gcccttcctt gcactctctg gtggcttcac aagaagaccc ccatcctttg tcgccgcggg
     6421 cgccaaccgc cgggcatcct ggcagaccag gcggacaatc ttccttggcc gcacagcgcg
     6481 cgggaggcgg ctcgttggga agacgaggcg ggtcgccatc gagttgattt ttgattttcg
     6541 tctcgaacgg tttgttcaag cttcccaaac tggtgatggg atctccagcg ttgtccaaga
     6601 cgctgatgta atacttcagg gcacccgtcg tggtgacatc gttgcaatcg atgttgccac
     6661 cccaaccatt gcgtttcttg gtaaggttca gttttttcca agagccccca aagggtttgt
     6721 agcggacaat gatttttccg accggcatcc cttcaggcac ggaaacccag atgggtacag
     6781 gagtattgac catctgttcg ctgacgggtt catgacccac ttcgccgtcg gaagttggtg
     6841 gcacatccac tgtgacagag cccgtgtcac cggaggaagc tctggctttc gccgcatcga
     6901 atgctttttg aatctccggc gtcacgaaat cagcgtcggg taccaccgcg ccatgcagac
     6961 gaatggcctg gacgaaggtt tcttctgcct ccgtgtcatt gcccaagctg gattgaacga
     7021 taccgaggtt gacgagtatc tgtgccttga ccgccgggga acaaccgctg tcgccacaag
     7081 tgtcgagcgc tttcttcaga ttggcttccg ctttgcggaa gtcagccatg acaaaatcat
     7141 tgttcatggt atcttggtcg atcttttttg cggcagcgtc cttgggtgcc gccacggcag
     7201 tgatcgacac cgctccgagc gcgaaaacag ttgccgaggt gagggctgcg agacgaaagc
     7261 gacggaacac gttggtgtct ccttgcgatg ctcgagacga ggggttgggc gatgacatga
     7321 tcgctcagtg agtctccgat tcgattctga tgaactgcac tctgcgattg agtgcgcggt
     7381 tttggtcggt atcgttgggc accaaaggtc ggtccatccc gtaaccctgg gaacgcaatc
     7441 ggtcacgttc gatgccacgg gcgatgagcg cttgcatgac cgcacccgcg cgcttttccg
     7501 aaagctcttg attgtagcgc ttgttaccgc ggttgtcggt gtgcccttgc acttcgatcc
     7561 tgatctttgg attctgtttc atgatctccg ctaccgcgtc gaccaccgga tagctttcag
     7621 gtcgaatatt tgccttgttg tattcgaagt gaatctgctg aaggatccga atctccttgt
     7681 ccgtcacgat ggcaagggac ggctttttcg gacatcccgg acggtctccg ccagcgggac
     7741 cagcttcatt gggacaaatg tcttcgaggt ccggcactcc gtcgccatcg ttatccaggt
     7801 cgggacatcc gtccgcatcc tcgaatccat ccggatcttc cggttcattg gggcacttgt
     7861 ccacgatgtc gacaatccca tcaccgtcgt tgtccaagtc gggacaaccg tcctcatcga
     7921 gataaccgtc catatcctca ggctcgagca cacacatgtc tcgtgagtcc gggattccat
     7981 ctccatcggt gtccggatcg tcaggacaac catcctcgtc ttgatatccg ttccacgttt
     8041 ctggttcgtc tgggcattga tcctcaggat caagataccc gtcgccgtcc cgatcccccg
     8101 gttttggctc gggtttttcc atgacgaaat cacgctccgc gcaaacttgc gggggagaca
     8161 gggagtaggc ggcgtgggtg ttgggttcgg caatttcgag atgccgggta gcacgcgaga
     8221 cgaagccctg gtcgagttcg agcaacgcga accgaaggtg ggacttggcc atcgccagtt
     8281 cgcgcggcgc acaacgaatg gcaccgtttc gttccgcttg gtcagcgatt ttttgcagcc
     8341 cctctatctt gcctcgcatc acgctgctcg tggtgcatgc gggagccaac aacaccgtgg
     8401 ccagcagcac gatcaaccct acgagtgggt aggcgctgcg acgaccaagg ttgcccattt
     8461 tcatccaccg ggtcgtcatc gtcccgctcc tcgatgggct tcgcgtgcca gccggatggc
     8521 tttatccgca tattcctcgg cgacttgcgc gagatcggcg gcatcgccgt aatctccctg
     8581 cgaagcttct tcttgggcct tcgtcatgtg ctcgagcgcg aaataatatt cgtacggagc
     8641 acgttcctca gcaccaactt ccttggcttg ctcgagcttg gcggcggcac cgcccgcgta
     8701 ggcagcataa aggacacctc cgcatccgct cgtagcgagt aaagcgcacg ccaccgcggc
     8761 tgcacgcgct gcgcttcgaa cgcgttgcat gatcaccaat gtttctcctc cgggtagtcc
     8821 cggtcgatcg acagcagggt ttggtgtctc catgggtcca accgctcacc tcgcagaaga
     8881 cgagcaggcg caagctatca ggacagctag gttttcgtca accgcgtggg catgaccgat
     8941 aggacaaatt gcaagttggc tagaagaaac agcaggacag taggccagca ggcccccaaa
     9001 tgcgatattg cagcattttt ccagcgatat cggcgggcct gcaagattct ggccccacaa
     9061 cccgcaggta agatatgctg ggaccagcgt gggaagccat atccgacagg aacaattggc
     9121 acgagccgaa atattttcgg gacttacctc cgaaggtctt cgtctgttgg ccgatatcgc
     9181 tatcgaagaa gaatttcctt ccgaaacgac gatattttcg catggtgcgc cgggcgataa
     9241 gctgtatctc attctcgaag gaaaagtgcg catcagtcgt accgtgcccg gcatgggaga
     9301 agaagccctg gctgtcctgc aacaagggga tgtgtttgga gaaatggcgc tgctcgatga
     9361 agctccccgg tccgccgatg ccaaggcgca cgaaccgtgc ctgctgctgt ccatcaccaa
     9421 ggaacgcttc gaagacctgc tatttttgca taaagacctg gcgtacgaag tgctctggaa
     9481 tgtgatccgc accttgatcc gccgacttcg agctaccaac gaaaaactcg catttctctc
     9541 catttctggt cggttctgaa aaccgtcttt ttgacagcga gtcacatgtt cagtaggata
     9601 acggagccat gcaagggttg acccatcgac aacaacaggt gctcgacttc attcgcgagt
     9661 cgatctcgga acgaggctat cctcctaccc ttcgggaaat cggtgcccat atgggcatcc
     9721 gctccaccaa cggggtcaat gaccacctgc gcgcgctcga acgcaaagga tatctgaccc
     9781 gcgaagacat gaaaagccgc gctctgcgcc ccaaagatag ggaccgggag gcaatcatcg
     9841 gtgatgacga taacgtgatc gaaataccaa tcctcggtcg cgtcgccgcc ggagcaccca
     9901 ttctcgccga tgaacacgtg atcgactcgg tccacgtcac gccgcgtatg cttcgcggcg
     9961 caaaagacgt ttttggcctt cgcacgagcg gcgattcgat gatcgacgct gggatcctca
    10021 acggggatta tatcttcgtc cgacggcaaa acaccgccaa ccgcggtgag atcgtggtcg
    10081 ctctcatcaa tgatgaagct accgtcaagt acttcttccc cgagaaagac tacatacgtt
    10141 ttcaacccgc caatgcgcaa atggctccca tcctcgtacg ggcttcagac ttccgctcca
    10201 cgatgatcct cggtgtcgtc gtcggtgttt tccgaaaact atgatcacgt gtcgtgggta
    10261 acgcctaacg agtgaacgtc actcgaatgg gtggcgtcgt tgccatgatc gcgttgccgt
    10321 cacgatccca tgcgggctga tatcgcttgg ataacgcaca catacgagca gcccgaccaa
    10381 atccgtggcc gggatcagat accacctgta ccgaacgcgc cgtgccatcc gcacgaaccg
    10441 tcaccaccaa cgtcacgacc gcgtagtcca cttgatccac atccgcttcc ggaggaaacg
    10501 gacaggatga ccagttcgtt ccctgggcaa tcgtcgcccc acgagaacga tccggccccg
    10561 aaagttgact tggagccgga ggcggtttgc gcgaagatcc cgtcgctcca ggcttgcccg
    10621 tcggtgatgc cgccgtgttg tacgtcggtg tcgtccccgt gcccgccgct gctgtcaccc
    10681 ctcccacaaa cgaatccgct tcccccgtga caaatccatc ccccgtcaaa tccaagggcg
    10741 catccggttg ctccggcgcg gtcagaagct ttcccgcttg cgccgccgcc ggagggggcg
    10801 aagcaggttc caccgccgcc ctgggtgcag gggttaccgg attagcctct tctttctctt
    10861 tcgccgcatc gtttttcgct tcgtctacca ccggctcagg tggcggcttc accacgtcaa
    10921 tatcgtaatt cgaccacaaa tactcatgaa ccacacgctg caccgactcc gcccaccatc
    10981 gcatttccgt gggcgccgca agcgcacgcc ccccaaaaaa accatgggtt atcgtcgcca
    11041 ccgtaaaccc aacgatcaat cccgtacgcg tcgcacgacg cccaaactct cgcaccgccg
    11101 ccagtgggtc accccggtcc ggaaccgttt tgagtttccg ccgacgaacg ggacgcgcca
    11161 tcgcctacta ctttccttcg ccgtctttcc gagactcgtc caccttgtcc acgggagtga
    11221 caccaaaagc aattttcgaa attccagcgg tcttgagcaa ttcgagcacg ccgatgacgc
    11281 gaccatgcgt gacggcttgc tcggcttgaa tcaccgcgcg caagtcttcg tttttcgaac
    11341 gcgcctcccg ggctagcccc aaaatcgctt cgtcgcccgg aagctttttc tgatccacga
    11401 acacatcccc attcttgtaa agctgcactg cgaaaatcat ctgaacgttc tggccgctcg
    11461 acgctttggg caagtccatc gggacggatt gagacacgat gatcttcgct gtcaccatca
    11521 tgatgatcag caacacgagc gtgatgtcca ccaacggaac gatgttgatc ccggtgatgc
    11581 catcggtgtc atccgtagag ccgctgatca tgacgactct cctcgttgct cctcagaacc
    11641 gggaggtgcg gccgtgcctg cgtcgaccgc atgcaggtac gataacagca ctccggaaag
    11701 cgtttccgta ttggcgagcg tcgatttgat gaggcgctgg aaaaagttga aggccatgac
    11761 cgcggggatg gcgacggcga gtccgacggc cgtggcgacg agcgcttcag cgatggccca
    11821 catcactgtc tggggagcca tgacagcggc ggcttctccg gcaggggctt gagagtgttt
    11881 tccgagttct tcgaaggcca tgacgacacc gatgaccgtg ccgaaaaggc cgatgaacgg
    11941 ggcgttgtta cccagggttc ccaagatggc tagacgtttt tcgaggcgaa tgcgctgcaa
    12001 ggccgcggct ccgcgcatcg cttcttccgc cgctttggga ccacgatcgg cctccatcaa
    12061 acccgcgacc accaccgccg cctccgccga aggagacttg cccatgtcgc tgatcgcccc
    12121 ctcgatatct cccttgcgca gtagttctcg aaggcgtttc gacaggttcg ccaagttgtc
    12181 tcggatcgac aagaagaacc acgcgcgttc caacatgacc gcgaccgaga cgatgctcaa
    12241 aatgatgagc aaccacatca cccaggccgc gccgaagccg accatgatcc gcttgagcca
    12301 ttcaacgata ttcattctga agaaatcctt agaccgttgg cacgctgttt agcgcgaaga
    12361 aggaccggtg tcgtccaaca tctcctcgtt cggcggtgaa tctgccaccg tcccccggcc
    12421 cgatgcaacc ttcgcggctt cgcttccatc ctcaagcaca aagaccgaac cgagtaccgt
    12481 ggtggcatag gcacccttgg gaagcaccat cgtaagccaa agacacgagt ttccttcgtc
    12541 ttcgaagaaa cgatagtcca gatccgaagg cagcacacgc aaggctcgcc ggcttcccgc
    12601 tcccgctttt ccaagagctt cgagcgcttt ttcacccccc agcgacgatt cgagcacttc
    12661 tttttcccac tgggcgggct ccccttgcgg ccatcgcatg cgaggaccaa atatcggacc
    12721 cgtcgccgat acctcgccga ccaacgcccg ccgagcgtcc gtttccggat cctccgtcag
    12781 gaataatccc ccatgagcag tggtttttac caagtccccc gctaccaccg tgttccacgt
    12841 gccctgctgc acccttttct cgagcagccg atcgaaaagc aacgattgca cggaagacaa
    12901 gagcaaacga cggatccttt tgtccttcgg accccggctt tgaccccgaa gccattgcaa
    12961 cgtttgctcc ggattgttcc catcgcgtcc gaatcgctga gggccgaaag cgttcggaac
    13021 tccttgggtt ttcagagtct ccaacgcttg ctgcagggcc gcctgctccc ccacgatgtt
    13081 gcgtaatacg agcgtaaaac ggttcccggc caaatgaccg acccgcagct tgtgcggatg
    13141 cctcgtgagt tgcaatgcct cgatcccctc cgacgcaagc tccgacatcg agggtaacgc
    13201 cgccgattcc ggccaaggaa acgacaaggt ctgctccgta accgcatggc gatccttcaa
    13261 tccggcatga cctatcgcgt cgttcgcgat acccaggtgc tgtgcaatac gccgcgcaca
    13321 agccgccgta gaccatcccg tttttctcaa ccgcaaaaat acatgaccgc cacttcccga
    13381 aggctcgtac agggcaagct cctcgacgat gaagtccgag ggggtcgagc gaatcgtggc
    13441 gcttggtcgt gaccgttcaa aggacatgat acgtttcaat cactccgttc atcgagggtc
    13501 ggcacaacaa cgaaacccgg tagagtagtc gtgatacgcg gcattgtggg ccgtggtttt
    13561 gtagttacag ccttcaccat tgattttggt gtccagataa tacccgccca aaaacacccc
    13621 gctcgtgtct tgtacccatt cgtgcaagtt acccaccatg tcgtacacgc cccacgcatt
    13681 tcgacatcgg gaaaatgcac ccgtctgcgc caacgtgccc cgaagctggt tgagcttggg
    13741 gtcattcatc ggtgaccatc cgtatgcgga tagcggtggc ccccccttga ccccgtaata
    13801 atggttcaga ggggataacc cgttgtcgtt gcaatgaccg ggcttatgct cctttccata
    13861 aggatatagc gtaggaactt tccccttgca cgccgtcttc cattccgtct ccgtgcaaag
    13921 acgcttgttg gatcgctcac aagccgccgc tgcctcgttt cgacttatat acgcttgtgg
    13981 ataccgacca ggacgcgaaa ccgcacgcac ctgcttgttc gccaccgact cgaaaggaga
    14041 atggaactcc cagcgattcc cttttttttc ctgagtcacc gcctcatacc gatcgataca
    14101 aaacgaaccg tcgaccaaca ccatgttcaa caaatgacag accggccgac gcgccgatgc
    14161 gggagttggc agcgcggcca cgagcaatcc cgcaagccct acgaagccaa ggctacgccc
    14221 ccttcgaaac aaaaaggcca tcataccctc gaaaaatccg agataacgac gatacaacct
    14281 gcggtccaac gatgccatgg tgatgcctga ccatcctgcc acaagatcgg cgttcgtcga
    14341 ccccgaccta ccaggttccc cattggctga accctacccg caggccgagc ccaacctggg
    14401 gaagtaccgt gcgcttcgtg taaatgcgct cctctccgtc tcgcatccca ttggctcctt
    14461 ccccatagcc ttgcaacaca aagtccaaca agacctttcc cacattgatc tgtgggccca
    14521 tgtcgagcgc cacataacta ttcgtgccct tgaattcgcg ccccgcaagc tcgagccggt
    14581 gccacacgac tcccgctccc aacgccgccg tgaatcgaat actccttccc cccaaaagca
    14641 aacgcaggtt tccccccgct cgatgaccgc tcaaatcgta cgtttgcggc atcccccgcc
    14701 aggtccccga aacactttgc gaacccgtgt cataggcccc ttctatcccc aaatacccca
    14761 atcgataccc cgcgcgtaat ccaaaatgac cgcctgtcac atccgcccca tcctccgctt
    14821 cgaacccatc cggagaacgc aataccgcca acaccgacgc catcaccgag ccgtaccaac
    14881 cccggtcttg atccaaccac gtgcgcccct tgcgataagg caacgtcgcc gggtcatagc
    14941 ctaacgacgt attcgctcct tcctgctccg ggcccaccaa taacctcaat tccaccacat
    15001 catcggcgcg caccctcacg cgggtcgcca ccgtggcgtg ccccgctttc acaatctcca
    15061 gaagatgctc acccgcctgc acctctcctt cccatccccc ccgacccaaa ggtttgccat
    15121 ctaccaccac caccgcatcg tcatcgtcca catccaccgc caaaagtcct accgcttgct
    15181 tcagctcgat gggaaccagc cgcgattcac cccccgccac cgcaatcgtc tgagtcacgc
    15241 ccatataatt cggagccgtg atttcaagac gatgcggacc cgaggacaat cgtatcggac
    15301 gcgccaactc ctccgaactc aatcgcttgc catccaccac gaccgttgcc gtccgcggtt
    15361 ccacctgtaa cgtgagcgtg ccgacgagct gagaaagatt gcgaagagcc tgctgcacac
    15421 tatccgcatc cttcttcgtc aacgactccc caaaacgttc gcgaagagtc tcgaatgtgt
    15481 cgatggcctc cacgtatcgg aaaagcttgg tctgacaaag cgcgatgttc tgaagagccg
    15541 cggccgaggc aagctccgaa taagaagcct caaattcaac caacgcggct tcgaagttct
    15601 gctcgtgata aagcgttacc cccgcgcgaa aatgtctctg cgccgcctcg cgatcttcgg
    15661 cgatcgccgc gttcgaaacg accataaggc agatccacca cagcaggcag accaccacgg
    15721 ctcgcttttt tactccacct ttcaaggcag ttccgtcttc aacgccaaac cacccgtgag
    15781 attacccgat ccaacctccg ccgggcgaga agtggttacg tttcccggcc acgaagcgga
    15841 aagagaaatg gatgcctacg gcagtaacca atcccgggca tccgcaatct accagtgctt
    15901 cgccgccttt tgctatcctc cttcgccacc atgacatccc ctcgcgaagt gctacgctcg
    15961 ctccgcctgt ttccacgacg acgccggcgt attccccttt ggctcgattc actcatggct
    16021 ttcgatcgag gcgtgtggat gctgtaccga ggcgtggaag tactttgcga cgaactgatg
    16081 ctcgcatggg tccccccatc gctctaccag gacttgatgc agcaaggata tgcacgagca
    16141 ggccactatc ttccaggcgg ca
//
LOCUS       DAOSOR010000050        16084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_3664_length_16084_cov_4.457109, whole genome shotgun sequence.
ACCESSION   DAOSOR010000050 DAOSOR010000000
VERSION     DAOSOR010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 16084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16084
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3664_length_16084_cov_4.457109"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PK710_04410"
     CDS             complement(<1..312)
                     /locus_tag="PK710_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HOT08987.1"
                     /translation="MWKLSRMPIILALVASALGLIFAGFSTYDYIQHLDRQLHAVHCS
                     FTPGLAEATDTENACRTAMYSVYGALFRGTWWGGVPVSLFALGVYVFFAAFAVAMLVG
                     GA"
     gene            complement(339..1634)
                     /gene="serS"
                     /locus_tag="PK710_04415"
     CDS             complement(339..1634)
                     /gene="serS"
                     /locus_tag="PK710_04415"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOT08988.1"
                     /translation="MLDLRYVVDHIDEVRAALSRRNPQTAASLDAITELAAQRRETIS
                     AVERKAAERNNANKEMSKLPKQSPEFIERREKLRIVSDEIKGLEKRKDEIESEISEIL
                     LGIPNLPDASTPTGSSEQDNPVIRTWGEKPLFDFEPKAHWDIGPALGIIDFERAAKLS
                     GPRFAVMMGLGARLERALTTFMLDLAREHGYLEVLPPFLVKDTALRGTGQLPKFEQDL
                     FKTRKSEVDESYDLFLIPTAEVPLTNLHANEILESSQLPLAYAAYTPCFRSEAGSYGK
                     DVRGLIRQHQFDKVELVRFCTPEDSPSQHELLTSHAEQVLQRLGLHYRVITLCTGDLG
                     FSANKTYDIEVWLPGLQAYREISSCSNFGDFQARRASIRYRDPQTKKPKLLHTLNGSG
                     LAVGRTVVAVLEQFQCKDGSVRVPDALRPYLGVDRIASR"
     gene            complement(1654..2178)
                     /locus_tag="PK710_04420"
     CDS             complement(1654..2178)
                     /locus_tag="PK710_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08989.1"
                     /translation="MARRLSTLWIPLGFFTLVGCSKPVQVHLTDTEGRHFEASCKDSS
                     CALIGAGEANPSASQPKGAKAAFVLHTASRLMAVCEVWVQGGAHGTHPADCRALTCQH
                     DRDCPWTKGLSTGTCTHGLCIEPSSEITNEDAVLLCLAGTGAPRGTTQQIERHALGNA
                     CATPCVVPSVCRQP"
     gene            2382..3281
                     /locus_tag="PK710_04425"
     CDS             2382..3281
                     /locus_tag="PK710_04425"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HOT08990.1"
                     /translation="MLWRVVGGIKTMRPHQWVKNVFVLAPVFFSKEFFHADLLLRAFG
                     AFCVFCLLASAVYTINDLVDVQADRAHPVKRYRPIASGRFPVTAAKVLAVVLVVISLA
                     GAAWGPIFFFATAVGYFVLNLAYSFRLKKVAYIDVGCIATGFVLRVLGGGFAAGILIS
                     WYLLACTALLALFLGFGKRRHELAQSTRKGSKTRVALDSYSPRALNIALVLTASATVV
                     TYAAYTLDPVIQRQFNSDWLWLTIPFTIFGVVRFLQLMAGRPRAESPTQEMLRDTLFV
                     FNIVVWALVAFAIVYRVRPEVGF"
     gene            3305..4024
                     /locus_tag="PK710_04430"
     CDS             3305..4024
                     /locus_tag="PK710_04430"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HOT08991.1"
                     /translation="MAGGLRALKQELGKLYSAKSDPWVDLGLTLPIFLGYHLGVVFLP
                     SRNAADIVTSQLVRLANFSILAYMGLTVALAASFVGLLVVLGRGKKLVWKRFVGVILE
                     GAVYAIVMSTTARFVVGSLHLAGTAEPRSAFSGLVMSLGAGFYEEVVFRVLLFGAGLA
                     LLQRAIPRREMLLGVGWAIASALIFSAWHYVGAYGDVFRLSSFVFRWVCGLAFVAIYR
                     FRGFAPVVWCHALYDIWVLAL"
     gene            4128..4862
                     /gene="uppS"
                     /locus_tag="PK710_04435"
     CDS             4128..4862
                     /gene="uppS"
                     /locus_tag="PK710_04435"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOT08992.1"
                     /translation="MNTLSGVPRHVAIIMDGNGRWAVQRGLPRQAGHKEGAKAVREVV
                     RGCRKAGVAFLTLYAFSLANWKRPSAEVNALMRLLIFFADQEVAELREREISVDVVGR
                     PEDLPTATRNALFELIRQTAGGTKMRLSLALSYSGRNDIVSAMQGIAREVEAGTLPAA
                     AVDESLLRHRMTTKDLPDPDLVIRTGGEYRLSDFLPFESVYAELHFPQILWPDFKEQH
                     LQEAFEAYASRERRFGKTSQQVAEEK"
     gene            complement(4907..5212)
                     /locus_tag="PK710_04440"
     CDS             complement(4907..5212)
                     /locus_tag="PK710_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08993.1"
                     /translation="MDIVRYQSCARPFLASSGSTSTIDPSSNSRSFRDVFHQLAREVD
                     RGERVVHAALSMGSQEDDARGMIALQAGVYRYVEAIELCAKLVDRTASAVKTTLQSQ"
     gene            complement(5276..5974)
                     /locus_tag="PK710_04445"
     CDS             complement(5276..5974)
                     /locus_tag="PK710_04445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT08994.1"
                     /translation="MTHVDPPEVMERFHRELTLVDVVAKQLYREVSDIIDVRELASFG
                     HEGLLMAARRYEPDRGIPFRRYAYYRVRGAILDGMRSCSALPRRAHEKVRALQAANVV
                     ASGFFDDSQDAAIRGLSPEDTDQRLSDHLAVLATAMALGFSGQESVLDGERVALSVDS
                     PDSSLETQELMQLVHQELPNLTPQEELFVRRHFFLDENIDTIAASLGLSKSWGSRILA
                     RAITKLSRRMQSRL"
     gene            6116..7117
                     /locus_tag="PK710_04450"
     CDS             6116..7117
                     /locus_tag="PK710_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT08995.1"
                     /translation="MNKARVLVIDGDDWVARLLADGLREHGFDVSTCLSGGDALAVAA
                     TIEADCVITEMVLPELDGPSVARAIRSEKPPLGFVPILFLSNADDMVSRSAAFQAGAD
                     VYLTKPFRLDEVALQVRGLVAMSTRLTGSEKSRRLPPIAAAEIPESISPSVHAIEGNV
                     AQMSVATVLTLLEMERRSGVLKVSEGKHKCELDIIAGYAVGGAIDASQVTPLAVLREI
                     LQWKEGRFRFIPGSDGAIPNNRRSIGALLIEAVRLDDEANREQWPEESSTARRSWPAP
                     GRTKKPVTSPKKPSRPPPPARLSQPPMPSNMGRAAPRPTAPRRARPAPPPAPSLKKK"
     gene            complement(7166..8782)
                     /locus_tag="PK710_04455"
     CDS             complement(7166..8782)
                     /locus_tag="PK710_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT08996.1"
                     /translation="MKRLHLRILISLVIVTAAGLWIHARSYDFFADDAFITLRYGRML
                     WEHGEPVYNLGQRVEGYTSPLWMVFAAIAHGLGDPVRVMQGFGGVGAVLWLIGLARLW
                     WQLEPQASQGAALVLVGTALSCPIAAWTMGGLETPLFGALLTWSIAEAAHLASRAPSL
                     RHALVTAMLLTLTTLARPEGALVATIVITLLLVLRFDRPHLRQTAVVGGVYALLTGTH
                     VLWRYGYYGYWLPNTYYLKSSGESAALMKRGWAYVVLAGQELGWPLVFLFALGLLLPW
                     LHTYPKEHPHIHAKRLILWSSFALWLCFVPYIIRIGGDFLDLYRFFAPLLPLGFVLVA
                     AAMHTTAAKLRLPWQALLALTTVLLGLHGFNQWKLKERAQSITEPARIAHGIEALGWT
                     RLYALRWAAMGRWVASFAKPDDWMAVGAAGAMPYFAGISNLDTFGLCDEFVAHHGAIV
                     ASRPGHQRFAPFDYMMRRRPTFVFVDDYSRDYPLRHLSTLAHYRREGYQLVEAHLTPA
                     MHTAPSEFFHYLYVRQDRAKQMEGQPAFRR"
     gene            8909..11119
                     /locus_tag="PK710_04460"
     CDS             8909..11119
                     /locus_tag="PK710_04460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT08997.1"
                     /translation="MTQPDSHPLVLSVIAPCFNEEFNVPALVERVLRVFDRGDLQGEL
                     ILIDDGSSDGTRRVIEEQQSQHPDRVVGRFHARNSGIAASWKTGVAVSRGAYVCIIDS
                     DLQYQPEDILRLFRTLIETSVDIVQGWRSAVGRERGTRYHLSRGLNTILNSAFDMSLR
                     DNKSGFVVCTREVMQDLLSYEGNYHFWQTFLMVAAHAKGYSYREIEILFENRRQGTSF
                     LDKGAYRASLLSFADIGKALWEYRFRRPVPDVATHFLKRYPVIDRTPKRGMVRDLEWK
                     AYLASFNHTHWMITRDVEHYYETLNKTQWLSMSQMRELQDEKLRRLIRHAYRNVPYYR
                     VKMQEAGVHPDDIRTQDDLHKLPLLSKQNVRDHLYFDIMSENHDKSEILKISTSGSTG
                     EPFVCYADRAQLEFRWAATLRSQEWTGYRFGDPCVRLWHQTLGMSKEQAIKERLDALL
                     VRRKFVPVFEMKEEMLAKTLREISDWQPTLIDGYAEALNLLAQFIHASEGLPHKPRGI
                     MSSAQTLPEASRRIIEDAFGCKVYDKYGGREFSGIAYECEAHDGHHVVGEGYIVEILR
                     DGKPAEPGETGEVVITDLNNTCMPFIRYRIGDLAEAVDNSRTCSCGRGLPRIGNIDGR
                     VQSIIQGIDGRYLPGTFFAHYLKEFDFAFKLYQVVQEEPDAMSFKVVKGSRFSNDILE
                     QVLATFRQYLGDSMRIDVEFVDDVAMVRTGKRLASVSRLPVDFQRNAPLKMSGI"
     gene            complement(11212..13242)
                     /locus_tag="PK710_04465"
     CDS             complement(11212..13242)
                     /locus_tag="PK710_04465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HOT08998.1"
                     /translation="MSNEFKPYVPEKTTLREFTVAALLLGLVMTVILGAANAYLGLRA
                     GMTIAATYPAAIIGMAVLKLLKGSILEENIARTVGSIGESIAAGAIFTIPAFVISGAW
                     DRDFGTFEAYAKASALMIVGGLLGIFFVTLLRRVMVNDKDLPFPESVAAAEIHKAGQK
                     GAGAAKQLFIAMAAGAGVYLLGAVKVFTASRDFVIHVGEIGKSTIRLGIEKSAETIKA
                     GGVATFSAPAISPAYLGVGYIIGPELGALNFAGGLLAWGLFVPILMYLLGPSFIAEHT
                     AAGGEALDWSSQALLVWKFVVRPIAVGGMLVGAANTLFKMRKSLATGIRRSISDLKKS
                     TQGDDAETGRLDKDLNMKVVFLGLILVLLAMVAVYFWFAGAIVPALVAAVVMAITGFF
                     FGAVSGNLVGTIGSSNNPISGLTLSTLIIAALLMVIVGAEGMAGVAAVLGVATVVCVS
                     SAVAGEMLQDLKVGHILGGTPWKMQVGDILGVVVSGAVMFFPLYILHSSNITEGGTGF
                     GDPKLPAPQAGLMAVLSQGIVGGEMAWPLVIVGIIMGLALVLVRVKSPMLVAVGMYLP
                     LETTFAIFVGGIIRWITDRFVESRKLNDAQKARTENTGVLVASGLIAGEALMGLVIAG
                     YVFFTDSPFPSIAWMGANWLAIGILVFLALLLIGIPLAHAGSPDEPAPPTAM"
     gene            13354..14211
                     /locus_tag="PK710_04470"
     CDS             13354..14211
                     /locus_tag="PK710_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT08999.1"
                     /translation="MPGFKPSISLFNRKTVAVVLASLFLVACPFFLMLFETSFIYYPS
                     RQILGSPSDEGLIFEDIHRTTSDGLRIHGWLMKPTSEPVAWLLFSHGNAGNISGRPRI
                     AQPLVARRVAVLLYDYRGYGKSEGNPDEQGTYLDGQAMLEELLSRARQPRQVFLFGRS
                     LGGGVSHELAVRHPELGGLITDATFTSIPQLARILFPIPGVWRVMRTRYDNLSKVPQI
                     KIPRLVMHGTQDEMIPFSMAEQLRDATNPPAQFFPISGAGHNDTFLRGGSEYADAIVR
                     FIEQCQSSP"
     gene            14284..15660
                     /locus_tag="PK710_04475"
     CDS             14284..15660
                     /locus_tag="PK710_04475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOT09000.1"
                     /translation="MTKERTEFTIPHRAGSIREVFSLAAPLMIAHASQSMMGVVDTFL
                     MGRVGNAQQGAVGLGGVLHWAAVCFFAGTMTIISVLVAQDFGAGRPDIARHVRAGLVF
                     VAPMSLLVFALIPWIPEALSVMHLAEQARPHAETYLQIRLWGAPLLLTAFALTSYLRG
                     IGDTLTPMGVALIANIFNALASAVLVFGWIGFPAMGVAGAAWGSVAASGLEVLLYAFA
                     YYFSPTARAHGSRRIILPRRTELSAFVWLGVPIGVAWLFEMVAWTSFSVYAGSRPPEE
                     LAAHTILFQFTGFCFMPAMAIGIAASTLVGQYLGAQRPDLARASAYSASAISVAFMLV
                     VGFALVWQRESLIHAFNRDATVVALGSSLAFIAALYQPFDGLGMVAQGVLRGAKHTAI
                     PTIVMLGSGLFVFIPLVWWLGTEKELGIRGAWIAALVHVVVVAILLGIAVMRSRVVHV
                     PTSLPVEA"
     gene            15734..>16084
                     /locus_tag="PK710_04480"
     CDS             15734..>16084
                     /locus_tag="PK710_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09001.1"
                     /translation="MSWKYIAPFGADPARVLAEAHKDAFERRAYGKPYSRREPRARSI
                     TALRRACAEDGTCSIIDVLRVEEEPSPGVAGRMPRGLIESVCGTPRPGLAQVEDALPI
                     LYEQLHRGEAGFVVA"
BASE COUNT         3416 a         4521 c         4649 g         3498 t
ORIGIN      
        1 ggcgcctccc acgagcatcg cgacggcaaa agcggcgaag aaaacgtata ccccgagagc
       61 aaaaagggaa acgggaacgc ctccccacca ggttcctcgg aacaacgcgc catacacgga
      121 gtacatggcg gttcgacaag cgttttccgt gtcggtagct tccgcgaggc ccggagtaaa
      181 cgagcaatgc acggcatgaa gctgccgatc gaggtgttgg atgtagtcgt aggtggagaa
      241 cccggcgaaa ataagcccca aagcagaggc tacgagggcc aggatgatgg gcatgcgcga
      301 taacttccac atggtcgacg ctcgttgcgt tcggtgggtc aacggctagc gattcgatcc
      361 actccgagat aaggacgaag cgcatcggga acccttaccg atccgtcttt gcattgaaat
      421 tgttccaata ccgcaacgac agttcgtcct acggccaaac cgcttccatt gagggtgtga
      481 agaagcttgg gttttttggt ctgggggtca cggtaacgaa tcgacgcgcg tcgcgcctga
      541 aagtctccga agttcgagca actcgagatt tctcgatacg cttggaggcc ggggagccac
      601 acctcgatgt cataggtttt gttggcggaa aaaccgagat ctccggtgca tagggtgatg
      661 actcgatagt gcaacccgag tcgttgcagc acttgttcgg cgtgagacgt gagcagttcg
      721 tgttgggatg gcgaatcctc cggggtgcag aagcgcacca actcgacttt gtcgaattgg
      781 tgttgtcgga tgagacctcg cacgtctttt ccgtaactgc ccgcttcgct gcgaaagcaa
      841 ggtgtatagg ccgcataagc gaggggtagt tgcgatgatt caaggatctc gtttgcgtgc
      901 aggttcgtca ggggaacttc cgcggtgggg atcaaaaata ggtcgtagga ttcatcgacc
      961 tcggacttgc gcgttttgaa aaggtcctgc tcgaacttgg gtagctgtcc ggtcccccgc
     1021 agggcggtgt ctttcacgag gaaaggaggc aggacttcga ggtatccatg ttcgcgagcc
     1081 aagtcgagca tgaaggtggt cagggcgcgt tcgagacgtg ctcccaagcc catcatcacg
     1141 gcaaagcgtg gaccggaaag ctttgcggca cgctcgaagt cgatgatgcc cagcgcggga
     1201 ccgatgtccc aatgtgcttt cggttcgaaa tcgaataggg gtttttcgcc ccaggttcga
     1261 atgaccggat tgtcttgctc ggaggaaccc gtgggagtcg atgcatccgg caggttggga
     1321 attccgagca aaatctcgga gatctctgat tcgatttcgt cttttcgctt ttccaaccct
     1381 ttgatttcat cggatacgat tcgcagtttc tcccgacgtt caatgaattc aggggattgt
     1441 ttcgggagct tggacatctc cttgttggca ttgttgcgtt ccgccgcttt gcgctccacg
     1501 gcgcttatgg tttcccggcg ttgcgcggca agttccgtga tggcgtcgag cgaagccgct
     1561 gtttgagggt tgcgacgcga cagggcggcg cgcacctcat cgatgtggtc tacaacatac
     1621 cgtaggtcga gcatggcgtg cgcctagcac tgctcagggt tgtcggcaaa cggagggaac
     1681 cacacaggga gtggcgcatg catttcccag ggcatgccgt tcgatttgct gggtcgtgcc
     1741 acggggggct cctgtccccg cgagacagag taacaccgcg tcttcattgg tgatttctga
     1801 agatggctca atgcaaagac cgtgagtaca ggttcctgtc gacagcccct tcgtccacgg
     1861 gcaatccctg tcatgttgac aggtcaaggc gcgacagtcc gcggggtggg taccatgagc
     1921 gccgccttgg acccatacct cacagacagc catgagcctc gaagccgtgt gaagcacgaa
     1981 agctgctttg gctcctttcg gctggcttgc ggatggattc gcctcaccgg cgccgatcaa
     2041 cgcacacgac gagtctttgc acgaagcttc gaagtggcgt ccttcggtgt ccgtgagatg
     2101 cacttggaca ggtttcgaac aaccgacgag cgtgaagaat ccgaggggga tccataaggt
     2161 tgatagtcga cgggccattg tgatcagcct agcagagtca tgaaggatca tggtttggct
     2221 ttgggttgaa tcactcctgg ttgatttgct gatttgacga aatgggtcaa cgcgtgccat
     2281 aggccaagga ccaggcgtgg acagagaatc cgatgggaga cgcgaatcac ctttcagagc
     2341 cgccgtttat ggcgagcgat ccacctccta gcacgggagg aatgctttgg agagtggtgg
     2401 gtggcatcaa aacgatgcgt ccacaccagt gggtcaagaa cgtattcgtt cttgcgccgg
     2461 tatttttttc caaagaattc ttccatgccg atttgctgtt acgcgcattc ggcgcatttt
     2521 gcgtcttctg tttgctcgca agcgccgtct acaccatcaa cgacttggtc gatgtacagg
     2581 cggatcgagc ccatcccgtc aagcgctacc gtcccatcgc ttcggggcga tttcctgtca
     2641 cagccgcaaa agtgctagcc gtcgttctcg tggtgatttc cctggcgggc gcggcatggg
     2701 gacccatctt ttttttcgcg accgcggtcg gctacttcgt tctgaacctt gcctattcgt
     2761 ttcgcctcaa gaaggtcgca tacatcgatg tggggtgcat tgcgacaggc ttcgtgcttc
     2821 gtgttctcgg cggggggttt gccgccggga tccttatttc atggtatttg ctcgcgtgta
     2881 cggcgctgct ggccctgttt ttagggtttg gcaaacgacg ccatgagttg gcgcagtcta
     2941 cgcgcaaagg ttcgaagacg cgcgttgcgt tggactctta ttctcctcgt gcgctgaata
     3001 tcgcgttggt cttgaccgcg agcgccacgg tggtgacgta tgcggcttac acgctcgatc
     3061 cggtgattca acgccaattc aactccgatt ggctttggtt gaccataccg tttacgattt
     3121 ttggggtggt tcggtttctt caactcatgg caggacggcc gcgggcggaa tcaccaaccc
     3181 aagagatgct tcgcgacacg ctattcgttt tcaacatcgt ggtttgggct ttggtggcgt
     3241 ttgcgatcgt ttatcgggtg cgccccgagg ttggattcta acagggactc cgaaggaggc
     3301 acaggtggct gggggcttgc gcgcgctcaa acaagagctt ggaaagctgt actccgccaa
     3361 gagcgaccct tgggtcgatc ttggtctaac cctacccatc ttcctcggat accacctggg
     3421 agtcgtcttt cttccatcac gaaacgccgc ggatatcgtt acttcgcaac tcgttcgact
     3481 cgccaatttc agtatcctgg cctacatggg cctgaccgtt gcgctcgcgg catcgttcgt
     3541 cggtctgttg gtggtcctcg gacgaggaaa aaaactggtt tggaagcgtt ttgtcggggt
     3601 catcctcgaa ggagccgtct acgccatcgt gatgagcacg actgcccgct tcgtcgtggg
     3661 ctcgttgcac cttgcgggga ccgcagaacc acgatcggcc ttttcaggac tggttatgtc
     3721 gctgggagcc gggttttacg aggaagtggt atttcgtgtc ctgttgttcg gcgcgggact
     3781 tgccttgttg caacgagcca tcccacgtcg tgagatgttg ctaggggtgg gctgggcgat
     3841 cgcatcggct ctcatttttt cggcttggca ttacgtgggg gcgtatggcg atgtttttcg
     3901 cctgtcttct ttcgtattcc gttgggtatg cggccttgct ttcgtcgcga tttaccgatt
     3961 tcgagggttt gcccccgtgg tttggtgcca tgccctttac gatatttggg tgttggctct
     4021 gtagaatgag gtcaatccgc caacggttct ggggctgctg acggtcggtt caatcccacc
     4081 aaagacagcg cttgtccggc acgcacgggt ggtgtaaccg cagcatcatg aacacccttt
     4141 ctggcgtccc gcgacacgtg gcgatcatca tggatggcaa tggacgctgg gccgtacaac
     4201 gagggttacc acgacaagcc ggtcataaag agggagccaa agccgttcgc gaagtcgttc
     4261 gaggttgtcg aaaagccggc gtcgcttttt tgaccctgta tgcctttagc ctcgcgaatt
     4321 ggaaacgccc aagcgcggaa gtcaatgcgc tcatgcgctt gctcattttc ttcgccgatc
     4381 aggaagtcgc agaacttcgc gaacgggaaa tctcagtgga tgtggtgggc cgtcccgaag
     4441 acctccccac cgccacacgc aacgcccttt tcgaactcat tcgtcagacc gccgggggaa
     4501 caaaaatgcg actgtccctg gctctcagct atagcggacg caacgatatc gtctccgcca
     4561 tgcaaggaat cgcgcgtgaa gtcgaagccg gtaccctgcc cgccgccgcc gtcgatgaat
     4621 ccctgcttcg tcatcggatg acgaccaaag atcttcctga cccggacctg gtgatccgta
     4681 ccggcggaga atatcgcctg tcggactttt tgcccttcga atcggtctat gcggaattgc
     4741 attttccgca aattttatgg ccggacttca aagagcagca cttgcaagaa gccttcgaag
     4801 cctatgccag tcgagaacga cgtttcggca aaacgagtca acaagttgcc gaagagaagt
     4861 gacgtagggc tcatccgcgg ccatcgaaag actttctcga cgggaatcac tggctttgaa
     4921 gcgtggtttt taccgcggat gccgttcggt cgacgagctt agcgcacaat tcgatggctt
     4981 cgacgtagcg atacacgccc gcttgtaaag caatcattcc gcgcgcgtca tcttcttgcg
     5041 atcccatgga taaagcggcg tgaacgacac gctctcctcg atccacctcc cttgccaact
     5101 gatggaaaac atcgcgaaac gagcgggaat tggacgaagg atcgatcgtt gacgtcgagc
     5161 cggaagaggc aaggaaggga cgagcacagg attgatagcg aacgatgtcc atgggcgctc
     5221 cttgggtgct cttttccttc ggccaaaccc tggcgtgggt tgcgtcgaat gacaattaca
     5281 atctcgattg cattcttcgc gagagcttgg tgatcgcgcg tgcgagaatg cgagatcccc
     5341 acgactttga cagcccgagg gatgcggcga tcgtgtcgat attttcgtcg agaaaaaagt
     5401 gacgacgaac gaaaagctct tcctgtggcg tgaggttcgg gagttcttga tgaaccagtt
     5461 gcatcaactc ctgcgtttcg agggaggaat cgggactgtc gacggataag gcgacgcgtt
     5521 cgccatcgag taccgactcc tgaccgctga atccaagggc catcgccgtg gcgagaaccg
     5581 cgaggtggtc ggataggcgt tggtccgtgt cttcaggaga aagaccacgg atggcagcgt
     5641 cttgggaatc atcgaaaaat ccagaagcta cgacattggc ggcctgaaga gcgcgtactt
     5701 tttcatgagc gcgacgggga agggcgctac aagaacgcat tccgtcgagg atggcacctc
     5761 gcacgcgata ataggcgtat cgtcgaaaag gaattccgcg gtccggctcg tatcgacgag
     5821 ctgccatgag cagcccttca tgaccgaaac tcgcaagttc acgaacgtcg atgatatcgc
     5881 tcacttcgcg gtaaagttgt ttcgcaacaa cgtccacgag ggtcaactca cgatggaatc
     5941 tttccatcac ctccggtgga tcgacgtggg tcacgcttgc acgtttcgtt atcagggcaa
     6001 tttctgtcaa gcaaagccca ccttggcagt ccattttctg ccggaacgct cccccgaacg
     6061 ccagaaaaca tggaaatggc tctccggttg gcaagtgtga taccgttatc cccgcatgaa
     6121 taaggcccgt gtgctggtca tcgatggcga cgattgggta gctcgactcc ttgcggacgg
     6181 attgcgagaa cacggattcg atgtcagcac gtgtctttcg ggcggagatg cgttggccgt
     6241 tgccgcaacc atcgaagccg attgcgtgat caccgaaatg gtgctgccgg aactcgatgg
     6301 tccttcggtg gctcgcgcca ttcgatcgga gaagccgccg ttagggttcg tgccgatttt
     6361 gtttctgtcc aacgccgacg acatggtttc ccggagcgcg gcattccaag caggagcgga
     6421 cgtttacctc accaaacctt ttcgcttgga tgaggtcgcg ttgcaagtcc gtgggctggt
     6481 agccatgtcc acccgtttga caggatcgga gaaatcacga cgattgcccc ctatcgccgc
     6541 cgccgagatc cccgaaagca tcagcccctc cgttcacgcc atcgaaggaa atgtcgcgca
     6601 aatgtccgtc gcgaccgtac tcaccttgct cgaaatggaa cgtcgctccg gagtgttgaa
     6661 agtcagcgag ggcaaacata agtgcgaact cgatatcatc gcgggatatg ccgtgggggg
     6721 agcgatcgat gcttcccaag tcactccttt ggccgtactg cgggagatcc ttcagtggaa
     6781 agaaggacgc tttcgcttca tccccggttc ggatggagcc attccaaaca atcgtcgttc
     6841 cattggcgcc ctgttgatcg aagccgttcg cctcgatgac gaagccaatc gggagcaatg
     6901 gcctgaagaa agctccaccg cacgacgttc ttggcccgcg cccggtcgaa cgaaaaaacc
     6961 tgtgacgtcg cctaaaaaac cgtcacgccc tcccccgccg gcacgcctat cccaacctcc
     7021 aatgccatcg aatatgggac gagcggctcc aagacccacg gcaccgcgtc gcgcaagacc
     7081 cgctcctcca cccgcacctt cgctgaagaa aaagtagcca atatgataac atattgattt
     7141 tgttgaatat cttgaatagc aggacctacc ttcggaacgc gggttgccct tccatctgct
     7201 tggctcggtc ttgccgaaca tacaaatagt gaaagaattc cgagggggcg gtgtgcatgg
     7261 cgggcgtaag gtgggcttct accaattgat agccctcacg acgataatgc gcgagtgtgg
     7321 ataagtgccg gaggggatag tctctcgaat aatcatcgac aaatacgaaa gtgggtcttc
     7381 gacgcatcat atagtcgaag ggggcgaagc gttgatgacc tggtcgagag gctacgatcg
     7441 caccatggtg tgccacgaat tcgtcgcata aaccgaaggt atcgaggttg gagatgccgg
     7501 cgaaataggg catggcacca gccgctccca cggccatcca atcatcgggt ttggcaaagg
     7561 aggcgaccca acgacccatg gcggcccacc gcagggcgta aagcctggtc cagccgaggg
     7621 cctcgatgcc atgagcgata cgcgcgggtt cggtgatact ttgggcacgt tctttcaact
     7681 tccactggtt gaagccatgc agaccgagca aaacggtggt caaggcaagg agcgcttgcc
     7741 agggcaaacg tagtttcgcc gctgtcgtat gcattgccgc ggcaacaagg acgaagccca
     7801 ggggcagaag gggcgcaaaa aaacggtaaa gatcgagaaa atcgccgccg atgcggatga
     7861 tataaggcac gaagcaaagc cataaagcaa acgaactcca aaggatgagt cttttggcgt
     7921 ggatgtgggg atgctctttg ggataggtgt gcaaccaagg cagcagcagg cccaaggcga
     7981 atagaaagac caaaggccat ccaagttcct gtcccgcgag cacgacatac gcccatccgc
     8041 gtttcatgag tgcggcggat tcccccgagg atttgaggta atacgtattg gggagccagt
     8101 agccgtagta gccgtatcgc cacagcacgt gtgtgcccgt caacaaggcg tacacccctc
     8161 cgacgacagc cgtttgccga agatgggggc gatcgaatcg cagcaccagc aacaaggtga
     8221 tgacgatggt ggcgacgagg gcaccttcgg gacgagccaa cgtggtgagt gtcaacaaca
     8281 tcgccgtgac gagggcgtgt cgaaggctgg gagcacgaga ggccagatgg gcggcctcgg
     8341 cgatggacca cgtaagaagc gcgccaaaaa ggggagtttc cagcccaccc atggtccatg
     8401 cggcgatggg acaggacagc gccgttccca cgagaacgag ggctgcgccc tgggaagcct
     8461 gcggttccaa ctgccaccac agacgagcca gcccgatgag ccaaagtacg gcccccacgc
     8521 ctccgaaccc ttgcatgacc cgaacgggat cgccaagtcc gtgagcgatc gccgcaaata
     8581 ccatccacaa tgggctcgta tagccttcca cccgctggcc gagattgtat accggctcgc
     8641 cgtgctccca tagcatgcgc ccataccgaa gcgtgatgaa tgcatcatcg gcaaaaaaat
     8701 cgtaggaccg cgcgtggatc cacaaccctg ccgcagtcac gatgactagc gaaatcaaga
     8761 tgcgaagatg caaccgcttc acaaggccat cctagtgacg gcatagaata gatcgtacaa
     8821 gattcgtgct ggtcccatga ccgacatcca cgcaactcgg gccgtctgtg ctacgcctgc
     8881 ctcatcttcg cccccgacga tgaacaccat gacacaaccc gattcccatc cccttgtcct
     8941 gtccgtcatt gccccatgct tcaacgagga attcaatgtc ccagctctcg tcgaacgcgt
     9001 tttgcgcgta ttcgatcgag gagacctgca aggtgagctg atcctcatcg atgacggaag
     9061 ctccgatggt actcgtcgcg tcatcgaaga gcagcaaagc cagcatcccg atcgtgtggt
     9121 aggccgtttt catgcaagaa atagcgggat tgcagcttct tggaaaacgg gtgtggctgt
     9181 ctctcgcggc gcgtacgttt gtatcatcga ttcggacttg cagtaccaac ccgaggatat
     9241 ccttcggtta tttcgaaccc tcatcgaaac cagcgtcgat atcgtacaag gttggcgcag
     9301 cgccgtggga cgggaaaggg gcacccggta tcacctgagt cgtggtctga acacgatcct
     9361 caattccgct ttcgacatga gccttcgcga caacaaaagt gggtttgtgg tctgcacccg
     9421 ggaggtcatg caagacctgc tttcctacga aggcaattac cacttctggc aaaccttcct
     9481 catggtcgct gcccatgcga aaggctactc ctaccgcgaa atcgagatcc ttttcgaaaa
     9541 tcgtcgtcaa ggaacctcct ttctcgacaa aggcgcgtac cgggcctcct tgctgtcctt
     9601 cgcggatatc ggaaaagccc tgtgggaata ccgctttcgc cgtcctgtgc ccgatgtggc
     9661 cacccacttt ctcaaacgtt atcccgtgat cgatcgaacg cccaagcggg gaatggttcg
     9721 cgacctcgag tggaaagcct atctcgcgag cttcaaccac acgcattgga tgatcacgcg
     9781 ggatgtggag cattactacg agacactcaa caaaacccaa tggctgtcca tgagccagat
     9841 gcgcgagctt caagacgaaa agctacggcg gctcattcgc catgcgtatc gtaacgtgcc
     9901 gtattaccgt gtgaaaatgc aggaggcggg ggttcatccc gacgatatcc gcacgcagga
     9961 tgaccttcat aagctcccgt tgttgtcgaa gcaaaacgtt cgcgatcacc tgtacttcga
    10021 catcatgtcg gaaaaccacg acaagtccga gatactcaag atttccacca gcggttccac
    10081 cggtgagccc ttcgtttgct acgcggatcg cgcgcaactc gagtttcgat gggcagccac
    10141 tctccgctcc caagaatgga ccggttaccg tttcggggac ccctgtgttc gtctttggca
    10201 ccagaccctc ggtatgtcca aagagcaagc catcaaagag cgtctcgacg ccctgctcgt
    10261 tcgccgaaag ttcgtccccg tcttcgaaat gaaagaggag atgcttgcca aaacccttcg
    10321 tgagatttcc gattggcaac caaccctcat cgacggttat gccgaggccc tcaatctgct
    10381 cgcgcaattc attcacgcct cggaaggatt accccacaaa ccccgcggca tcatgtcttc
    10441 ggcacaaacc ctgcccgagg cgagtcgtcg gatcatcgaa gatgcttttg gatgcaaggt
    10501 ctacgacaaa tacgggggtc gggagttttc cggtatcgcc tatgagtgcg aagcccacga
    10561 cggtcatcat gtggtcggtg aaggctatat cgtggaaatc cttcgggatg gtaagcccgc
    10621 ggaaccaggc gaaacaggcg aggtcgtcat tacggacctg aacaatacct gcatgccgtt
    10681 cattcgctat cgcatcggag acctcgcgga agcggtcgac aatagccgta cgtgctcttg
    10741 tggacgtggc ttgccccgca ttggcaacat cgatggtcgt gtgcagtcga tcatccaagg
    10801 catcgatggg cgatatcttc caggtacgtt tttcgcccac tatctcaaag aattcgattt
    10861 cgctttcaaa ctctatcagg tggtgcagga agagcccgat gccatgtcgt tcaaggtcgt
    10921 gaagggctcc cgcttctcca acgatatcct cgaacaggtg cttgcgacct ttcgccaata
    10981 cctcggcgat tccatgcgta tcgatgtcga attcgtggac gatgtagcca tggtccgcac
    11041 aggcaaacgt ttggcgagcg tgtcgcgttt acccgtggac tttcaacgca atgcgccgct
    11101 taagatgtcg ggaatataga aataatttag tgtattcaac tggttacgaa catggcgacg
    11161 agccgggggc tggcctttcg aatggcagcc ctcgaagcag aaggcggcaa actacatggc
    11221 ggtaggggga gccggctcat cgggggaacc ggcatgagcc aggggaatcc cgatgagcag
    11281 caaggcgaga aagacgagaa tgccgatggc gagccagttc gcgcccatcc acgcgatcga
    11341 aggaaatgga ctgtcggtga aaaagacata gccggcgatc accaatccca tgagcgcttc
    11401 gccagcgatc aaccccgagg ctaccaatac accagtgttt tccgtgcgtg ccttctgcgc
    11461 atcgttgagt ttgcggcttt cgacgaagcg gtcggtaatc caccggatga tgccgccgac
    11521 aaagatggcg aacgtggttt cgagaggcag gtacatgccg acggctacga gcatggggct
    11581 tttcacgcga acgagcacga gcgcgagacc catgatgata ccgacgatga caaggggcca
    11641 tgccatttcg ccgcccacaa taccttggga taagacggcc atcaggccag cttgtggtgc
    11701 gggaagtttg gggtcaccga agcccgttcc gccctcggtg atattcgaag agtgcagaat
    11761 atagagggga aagaacatga ccgcgccgga gacgacgacg ccaaggatat cgcccacctg
    11821 cattttccaa ggggtgccac cgaggatatg accgactttg aggtcctgga gcatttcgcc
    11881 agcgaccgcg gatgaaacgc aaaccacggt ggccacgccg agaacggcgg ccacgccggc
    11941 catgccttcc gcgcccacga tgaccatcaa cagcgcggcg atgatgagcg tggagagtgt
    12001 gagcccggaa atcgggttgt tggaggagcc aatcgtaccc acgaggtttc cggatacagc
    12061 accgaagaaa aagcctgtga tggccatgac gacggcggct acgagggccg gaacgatggc
    12121 gcccgcgaac cagaagtaca cggcgaccat cgccaacagc acgagaatga gacccaagaa
    12181 cacgactttc atgttcaagt ctttgtccag acggcccgtt tccgcgtcgt ctccttgcgt
    12241 gctctttttc aaatccgaga tcgatcgtcg aatgcccgta gccaagcttt ttcgcatctt
    12301 gaacaaggtg ttggcagcac cgacgagcat gccaccgacg gcgatggggc gaaccacgaa
    12361 cttccacacg agcaaagctt gggaggacca atccaacgct tcgccgccag ccgcggtgtg
    12421 ctcggcaata aaagaaggtc cgagcaagta catgaggatc gggacaaata ggccccaggc
    12481 aagcaaacca ccagcgaagt tcaaggcacc gagttcaggg ccgatgatat atcccacacc
    12541 gagatacgcg gggctgatgg cgggagccga aaaggtggcc acaccaccgg ctttgatggt
    12601 ttctgcagac ttctcgatac caaggcgaat cgtgcttttg ccaatctcgc ccacgtgaat
    12661 gacgaagtcg cgcgaggcgg tgaagacctt gaccgcgccc aacagataca cgcccgcacc
    12721 cgctgccatc gcgatgaaaa gctgcttcgc ggcaccggcc cccttttgcc ctgccttgtg
    12781 gatttccgcc gcggccaccg attcgggaaa gggaaggtcc ttgtcgttga ccatgaccct
    12841 tcgcaacaac gtcacgaaga atatgccgag caatccaccc acgatcatga gcgcggaggc
    12901 tttggcatag gcttcgaaag ttccaaagtc ccgatcccag gctccggaaa tgacaaatgc
    12961 agggatggtg aagatcgctc ccgccgcgat cgactcccca atggagccca ccgtacgtgc
    13021 aatattctct tcgagaatcg atcctttcag aagtttgagc accgccatgc caatgatggc
    13081 ggcgggatag gtggcggcga tggtcatgcc ggcgcgtaag ccaagataag cattcgctgc
    13141 ccccaggatg accgtcatga ccaaaccaag gagaagggcg gcgacggtaa actcccgcag
    13201 cgtcgtcttc tccggcacgt agggcttgaa ttcgttggac acaagtcctc cgatcgtggt
    13261 gatgggttga ctgcgaaccg actcgctgcg tactcagctt ccggttttct gttttggccc
    13321 aaacaacggg cctcccccct ttaccattgc gtcatgcccg gattcaaacc atcgatatcc
    13381 cttttcaaca ggaaaactgt tgccgtcgtt ctcgcttcct tgtttctcgt cgcttgccct
    13441 tttttcctca tgcttttcga aacctccttc atctattacc cctcccggca aatcctcggc
    13501 tccccgagcg atgaaggact cattttcgaa gatattcatc gaaccacctc cgatggtctg
    13561 cgcattcacg gttggctgat gaaacccact tccgaacccg tggcctggct cctgttcagc
    13621 cacggaaatg ccggaaatat ctccggacgc ccccgcatcg cccagcccct cgtcgcacga
    13681 cgtgttgcgg tattgctgta tgactaccgg ggttatggaa aaagcgaagg aaacccagat
    13741 gagcaaggca cctatctgga tggacaagcc atgctcgagg agcttttgtc ccgagccagg
    13801 cagcctcgtc aggtttttct tttcggtcgc tcgctcggag gtggcgtaag tcatgaactc
    13861 gccgttcggc atccggagtt ggggggcctc atcaccgacg ccacgttcac gagcattccg
    13921 cagcttgctc gtattctatt tccgattccc ggggtgtggc gagtgatgcg cacacgctac
    13981 gacaacttgt cgaaagtacc gcaaatcaag attccccggc tggtgatgca cgggacccag
    14041 gacgaaatga taccgttttc catggccgag cagttgcgcg acgccaccaa cccccccgcg
    14101 caatttttcc ccatctctgg tgcagggcat aacgacacat ttcttcgagg cggtagcgaa
    14161 tacgccgatg ccatcgttcg ttttatcgaa caatgccagt catcgccatg acgcctgttt
    14221 ttcgtcggct tttcgacagc acggcgcgca tgcgctagcg tttttgcttc ctcggcccga
    14281 accatgacca aagaacgtac ggaattcaca atcccccacc gcgctggatc gatacgggag
    14341 gtcttctctc tggcggcacc cttgatgatt gctcatgcga gccagtcgat gatgggcgtg
    14401 gtcgatacgt ttctgatggg cagggtgggc aatgcgcaac agggagccgt gggcctgggt
    14461 ggcgtgttgc actgggccgc cgtttgcttt ttcgccggca cgatgacgat catcagcgta
    14521 cttgtcgcgc aggattttgg cgccgggcgc ccggatatcg ctcgccacgt gcgcgcgggg
    14581 ttggtcttcg tggccccgat gagtttgctc gtcttcgcct tgattccgtg gataccggaa
    14641 gcgctttccg tgatgcatct ggccgagcaa gcccgtcccc acgctgaaac atacttgcag
    14701 atccgccttt ggggcgcgcc cctgctgctt acggcctttg cgttgaccag ctacttgcga
    14761 ggcatcggcg atacgctaac accgatgggt gttgcattaa ttgctaatat tttcaacgcg
    14821 ttagcttctg ctgtcctggt gtttggatgg atcggatttc cagcgatggg cgtagcaggg
    14881 gcagcctggg gaagtgtagc agccagcgga ctcgaggtgt tgctttacgc cttcgcctac
    14941 tatttcagcc cgacggcccg agcccatggg tcgcgtcgga tcatccttcc tcgacgaacc
    15001 gagctgtcgg cctttgtttg gcttggcgtt cccatcggtg tggcctggct tttcgagatg
    15061 gtggcctgga cttcgttttc cgtttacgcg ggaagccgcc ctcccgaaga actcgccgcc
    15121 cataccatcc tttttcaatt taccggcttc tgcttcatgc ccgcgatggc cattggtatc
    15181 gctgcttcca cgctggtggg ccagtatttg ggcgctcaac gccccgatct tgcccgagcc
    15241 agcgcctaca gcgcttcagc gatcagtgtg gcgttcatgc tggtagtggg atttgctctc
    15301 gtttggcaac gagaatccct gattcatgct ttcaaccggg atgccacggt ggtggcgcta
    15361 ggttcgagtt tggcgtttat tgcggccctg taccaaccct tcgacggact cggcatggta
    15421 gcgcaaggtg tgcttcgcgg ggcaaaacac accgcaattc ccaccatcgt catgcttggc
    15481 tccggacttt tcgtgttcat ccctctggtt tggtggctcg gcaccgaaaa agaattggga
    15541 attcgtgggg cttggatcgc ggccctcgtg catgtcgtcg tcgtagcgat cctgctcggt
    15601 atcgccgtga tgcgaagtcg cgtggtccat gttccgacct ccttgcccgt ggaggcttga
    15661 agcaggcaat tcttcgggat aatctcggga aaatcagggt atagacgaaa ggtgcgagga
    15721 ctctatgggc ggcatgagct ggaagtatat cgcgcctttt ggcgctgacc ctgcacgcgt
    15781 tctggccgaa gcacataagg acgctttcga gcgacgcgcg tacggtaagc cctattcgcg
    15841 ccgtgagccg agggctcgaa gcatcaccgc gctgcgtcgc gcttgcgccg aggacggtac
    15901 ctgcagcatc atcgacgttc tgcgggtgga ggaggagccg tcgccgggtg tggccggcag
    15961 aatgcctcgg ggcttgatcg agtccgtttg cggaacgcct cgccccggtc ttgcgcaggt
    16021 cgaggatgct ttaccgatac tctatgagca gctccatcgt ggggaggccg ggttcgtcgt
    16081 cgcc
//
LOCUS       DAOSOR010000051        15934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_3704_length_15934_cov_3.809749, whole genome shotgun sequence.
ACCESSION   DAOSOR010000051 DAOSOR010000000
VERSION     DAOSOR010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 15934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15934
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3704_length_15934_cov_3.809749"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PK710_04485"
     CDS             complement(<1..294)
                     /locus_tag="PK710_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09002.1"
                     /translation="MRCWLGLGGVVAGLLLGNVAHGQEGPGLAANYPNDIGLGVHPDV
                     LYFLDFDDETASRAWFGDNIGFGWTSEPDHVRSGKGALEIQHIQGTHAPTEIHP"
     gene            complement(417..719)
                     /locus_tag="PK710_04490"
     CDS             complement(417..719)
                     /locus_tag="PK710_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09003.1"
                     /translation="MKAGESKLRRVGRAALWVTALTAWTWLWFFPLSGFHHMVRCGLL
                     GWAILDWESGNLAFRWVASVLALSIALWAAGVAPVVIFLIFRYKRHRAEKLYYIEK"
     gene            complement(963..1793)
                     /locus_tag="PK710_04495"
     CDS             complement(963..1793)
                     /locus_tag="PK710_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HOT09004.1"
                     /translation="MTDHIPKPLLPIGDRPAFHHIIEQLRSFGVGPIAMNTHHRAEAF
                     EDKIPFEINVLHETRILGTAGGVANAQGVLGRGDVLVWNGDMQLDVDLPGLLARHAQA
                     VRTLEAAATLLVARRAAGQGTVGVSRVAAVARLRGERFSEEEAGGDYLGVMVLTERLR
                     ERLPPEGCLVGDALLPWLRQQGRVGVAWHEGEWVDIGTPEQYLRANLMWLKKRGISSW
                     VAPSAVMNPRVRLRECVVGEGSEVKGEGELERCVVLAGAVMQAPAQRMIAMPLGVLAV
                     "
     gene            1831..2037
                     /locus_tag="PK710_04500"
     CDS             1831..2037
                     /locus_tag="PK710_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09005.1"
                     /translation="MADKGGMIFSTTSTAGSEASYQNSPSLSLPSLFCRAGQVPLLVR
                     HGVIGITTPPVPDWLCRHGERDTP"
     gene            complement(2071..4152)
                     /locus_tag="PK710_04505"
     CDS             complement(2071..4152)
                     /locus_tag="PK710_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09006.1"
                     /translation="MAENGKLGTDSRRWWLGFFRSCGWDGQAVYVALIAALFLAFLGC
                     GGESEPADDEDASVGEESGDELLDAPGETEVLPEGAPPVACKPKSCVQVGATCGSIPD
                     GCGDKVECGTCPAGQFCGGAGPNTCGTERCIPKTCLQLNAQCGWVSDQCGEAIDCGGC
                     AYPQTCGGGGENNECGCTPKTCSQLGVNCGTVLDGCDGTLNCGECDPGQICGGAGPNL
                     CGGADCAAKTCSQLGAACGLISDGCSDVLFCGDCKAPDVCGGGGIANECGCTAKTCSQ
                     LGASCGIVDTDCGPLDCGDCKPPNTCGGAGVANQCGCTCSLPNAVTDCFYGECILVSC
                     LPGFDDCDGNPANGCETNLDTDPGHCGTCTHACPTMAHAQSTCQSGNCGFVCQGRFAD
                     CDGNASNGCETDLNTDPSHCQQCGNVCPDRAHATTFCAGATCGFQCLSGYGNCDSNAS
                     NGCETDLKTSLSHCGTCGKTCSFSNASASCTNGVCALKSCNSGYGNCDNNSENGCEVN
                     LATNKNHCGSCGRACPSGSCVSGTCEYTSADTARKWDQSYLGSGGQYNYNQQNNYQGQ
                     YLESNGVLKSAIGAPDKMFTDLAGAQVLDIWVYCYAAHWYWNSGGVARIGVHGHSTKP
                     GTFPGINQTLEVNFGKPEGKWVRLPDSWYASFKSGAYRGITLHAGNSTNLLYYGYLTA
                     AQTKWRWHYRK"
     gene            complement(4333..6114)
                     /locus_tag="PK710_04510"
     CDS             complement(4333..6114)
                     /locus_tag="PK710_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein"
                     /protein_id="HOT09007.1"
                     /translation="MSDKNSLSLSRGVPARASGGRGELWEECPSDDPFRGSNLAVPDI
                     LHEKTQAWTDLASVAGLVRFAFEGGECTEIFDELVRATPLSPSNWHAETFAGHLFLDD
                     LIRTCFPLEIDGIPHRIHEAFLVRALSHPPSNPADTRVRQEVFSELLEQPVIRSDLER
                     VYRAVARLRSHLDSTLQEEPEPIARKLEILRAVQACVEALAEGFGQATSLLSRLREAG
                     QRMRGSQGYERLRQLLDLEGNLATVDVRLRLGADGHVRELALRSVKENEQNPLLPSVW
                     QRVWQRLMAVVRGHRYGQREIVVSLFHEVFSSLVDTVVVCLALVGPMQFYLAGLGFRD
                     RSTRRGFSVCLPEIVDAACPSENRVIERVLEGVFNPLLELQGVEPRPVDVRLGCDDAL
                     VLLTGPNSGGKTRLLQAVGLVQCLGQGGVFVPASRARLIRAPTLFVSLVDEVDVAQTE
                     GRLGTELVRIRRLFEELQPASMALIDELCSGTNPTEGEEIARVVISLLPRLRPQVFIS
                     THYLGMAQQLERERPVRGLAFLEVELDGDNQPTYRFVPGVAKTSLAHLLAERLGVTRE
                     ALERIVAEKIGGWDVWVGGSWSGKDGE"
     gene            complement(6184..7209)
                     /locus_tag="PK710_04515"
     CDS             complement(6184..7209)
                     /locus_tag="PK710_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09008.1"
                     /translation="MNRSWPCLPLVGLSLWLAACGTDEQTSSPSDVHADAGTDAPGDS
                     TPAEDALAETNSSADAPSDTPSQGEGGPAEDVGEASSEASSDAPVQNDVAEADVTLVE
                     LSETEPNDGATLEEYNELPLDADMRGSIGTVGDADIFRVQAEAGKVVRVVVWLPPGSA
                     LEPHLVVFDDGRGNKAPGDDYVKVAVGKPATLEFLAMDSGGYYVAVRDVRNLSSPAVG
                     GVDFTYSLQATEHATSAFEGQPLVFPSHFTDSLGSPSGLRLYAFTGSKGADIVVDLKA
                     KGDMDGRLMIYADAISSWIARNDDRGAQDKNPLIDAFLTEGGPMFLVVENIHEKANNL
                     SYTLDVP"
     gene            complement(7371..10796)
                     /locus_tag="PK710_04520"
     CDS             complement(7371..10796)
                     /locus_tag="PK710_04520"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOT09009.1"
                     /translation="MFAAFRRWPFRRAPIIVAALFSGGASLAYEVAWSRALVVPLGNA
                     SDAAAIVLAAFMIGIGAGAWIGGEWAERTRFPLRIYALMELLLGAYAVVAPPLLSSLS
                     QIPGESGALLRRLLALLLIALPCLAMGMSLPLLVRAWTRKGRTLRWTVGLAYAANTAG
                     AAMGAWITGYWGLALLGVARWSALAALGSFSAAFLATLAESFRPSTPPPDSLSPEILP
                     QTTPTTVQRLAGLATFIMGFVVLANEVLWTRVLTFVFGHDTYAFATMLAVVLVGMALG
                     GALHRLVASADQAAWTAGLLGGFSVASLVSFWLAAHLVIHFGRDPFRLESSPYLAGSV
                     FIEIFRELTFTPLLLFLPCLLAGAAFPAACSLYAHRSESVGRGLGRVTFVNGLGSALG
                     AITTSLLLGTVFSVQGAFFALAILSALMAAVVLFFTRPRCFRTAGWALLPIAAVGLLA
                     LWIPSAMPRAMLLATVGPRHQTLLHYEEARTGTVSVVENRINQERQLLMNGINEVTTR
                     LVHDQSFKVLGHLAPLLHPNPKKGVMICLGAGISAGAALAHPLDSLDVVDLSSAVARG
                     ARYFEKQNRGVLDDPRFHLHIDDGRQYLLNTSERFDVAMIDSTHPKAVDSWILYTVEF
                     YELVRDRLSEGGIVVQWLPLHGLSEREFKMIVRTFQQAFPSMTLWANVGFETYGQVGY
                     AKLVGIRGRPLAIDPAQIAAKLRDSTIGKDLADWGVESVEELLNLFIAGPASLEAWTR
                     GLPVQHDDRPMIPYRTAFSLGRRMVPARLLAVREPISPWLVKASPDQKQAIVRSAEGQ
                     GLVLGGLLSRARELDVSNHSVPLYEHQIATTLPYYEALAALYPDDADRQFEAATQLAA
                     LGHPSHAGRYYERALALRPEDYRFRLDYGHWLLGLGRGKEAIEVLSSLRDDVPRAALV
                     HYNLGAAVLASGEAGAAAGHFETALGYDDTLYGARIGLARARIALSEEEKAQRALARS
                     QADNPWDEDVMHLWGILRTRQGRWEEAESLLRKAHRWAPYRSDILLDLGRVQARRGNL
                     RDAEKSYVLLLRYAPQDPAGWLGLADAMAASKRWAEAAESYAKVLELDSVSVAAALGL
                     GESLEKLQRNDSARDAFCLALHLQPGQQRAQAGLKRLEADCSGNASNDDHTPK"
     gene            complement(10983..12341)
                     /locus_tag="PK710_04525"
     CDS             complement(10983..12341)
                     /locus_tag="PK710_04525"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT09010.1"
                     /translation="MKSTDVLVIGGSAAGMVVALSGKGAYPDKRFTMVKKQDVVMVPC
                     GIPYIFGTLAGSEQNIMPVDKMMSQAGVESVVGEVVSIDIPKKVATFADGSTIGFDKL
                     VLATGSTPILPKWLEGREKENVFVVPKEKEYLDQMKAKLSACKHVVVVGAGFIGVELS
                     DEFKKTGGHVTLIEKLPHILALAFDVELSDKMTTLLQQRGIEVKTGVGVAKIGGETKV
                     ESVTLDNGDVIPADAVVVSVGYEPNSKLAKDAGIDIDENNFIDVDEYMRTHTPDVFAV
                     GDCAQKRDFVTRRRVPTMLASTACAEARIAGRNLYQLHTVKTFSGTIAIYATAIGDVG
                     FGTAGLTEARAKAEQIDVVTGIFEGIDRHPGTLPGVQKQTVKLIAARNSGVIIGGEVI
                     GGPSAGELTNVIGLIIQNRMTVNSLLIAQIGTHPCLTASPAGYPLIKAAEIMEKRMRD
                     RT"
     gene            complement(12639..14087)
                     /locus_tag="PK710_04530"
     CDS             complement(12639..14087)
                     /locus_tag="PK710_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09011.1"
                     /translation="MTSLRGFDRLEVGPVSLEPRALSAPYAVFHGSQKDEITLQYRYE
                     QDVFDADDAADHNLGTMMMAQAALNYGLFCDQIVIHGPVDTVDLALLRGMLRNTNREI
                     LVHKLCQPNPFLLPGRIEINASDIPSLEGAPLLSPRLSNSRIVAPWEHDNHRVAVLSS
                     GGKESLLTFGLMRELGFEVHPIYINESGRHWFTALNAHRGFKNTVPTTQRVWTNIDRV
                     YNWMLRHLPFIRQDFAHVRADQYPIRLWTVAGLIVGSLPLLRARAIGRLLIGNEYDTS
                     LRLSFHGLSHYGGLYDQSHWFDAALSKYYDKKRWGIKQFSILRPLSELLVQKTLAQRY
                     PQLQAWQVSCHAAHLDEEKALPCGRCEKCRRVVGMLEAIGADPGRCGYTAAQIEQTMA
                     ALASQSLHQDAPTSEHLLWLLTQRGKLPQPSRRRARPHPEVMKLRFDTLHAPFDLIPL
                     DLRQPLLRLMLEHADGALIRQHRTWAPFDPRE"
     gene            complement(14084..15172)
                     /locus_tag="PK710_04535"
     CDS             complement(14084..15172)
                     /locus_tag="PK710_04535"
                     /inference="COORDINATES: protein motif:HMM:NF019375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HOT09012.1"
                     /translation="MKPRAIVYCESAFRTTHGKTAHGLVRRSERFQIVGVVDSSCAGC
                     HAHDVVDGANPDIIVFENFQSALEASRRADRAASALIIGLAPDGGRLPASALHDVAVA
                     LNEGLDVYNGLHDWLNDDPALASLAAQHHARIIDIRRPPDRDQLHFFSGLIKQVDSFR
                     VAVLGTDSAVGKRTTAWAIVDAFRQANVSAQLVGTGQTAWLQGAEYGIILDSIVNDFV
                     AGELENAVYRAWKQAHPDVIVLEGQGSLLHPAYPGGFELLAATRPHVVVVQHAPGRQT
                     YDGFDDFPLHPIALQCQAIELISQRPVVAITLNTEQIPANDIPSICEQIEKQTGRPTM
                     APLVQGVDRLITLLRTHQRSWESTCHPR"
     gene            complement(15169..>15934)
                     /locus_tag="PK710_04540"
     CDS             complement(15169..>15934)
                     /locus_tag="PK710_04540"
                     /inference="COORDINATES: protein motif:HMM:NF004996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOT09013.1"
                     /translation="TLATCGNYGAAAGLAASLAGLRCYVYIPASYRTRRIAEIERTGA
                     ILIRTNGDYENAVELSRQHARKDELYDANPGGDNTALQLMAYGSIAYEIYDELRDAPA
                     AVAVSVSNGTTLAGLYRGFLSLYRRGKTSRIPRMIAGSSHNKNPIVYAFRHNLLRCDD
                     LRPKSIRETKVNEPLVNWHAVDGEAALEALRATHGIANDASDKEMSTLSKSLRSAEGL
                     DVLPAATAGLHALIELHRESNLPSDRYVVVLTGRKG"
BASE COUNT         3538 a         4737 c         4422 g         3237 t
ORIGIN      
        1 ggggtggatc tctgtggggg cgtgagttcc ttggatatgc tgaatttcga gcgctccctt
       61 tccactgcgc acatggtcgg gttctgaggt ccacccaaat ccgatattgt ccccaaacca
      121 agcgcgcgag gcggtttcat cgtcaaaatc caaaaagtac aggacatcgg gatgaacccc
      181 caacccaata tcgttcggat aatttgcagc caggccgggc ccctcttgtc catgagccac
      241 gtttcctaac aacagtcccg cgacgacccc acccaatcca agccaacacc tcattcggcc
      301 atgataacag aaacgagagc cgcagcaacc actccgatgt tttctcgatg tgggttgcca
      361 ccgtgcggac tcgagcctag catccgcccg cggcagaagg actatgctct tgagttctat
      421 ttttctatat aatatagttt ttcagcacga tgtcgtttgt atcgaaagat tagaaaaatg
      481 acgacgggag caacaccggc ggcccagaga gcaatgctca gcgccagaac ggatgccacc
      541 catcgaaaag caaggtttcc gctttcccaa tcgaggatcg cccatccgag caatccacag
      601 cgcaccatat gatggaagcc gctcagaggg aaaaaccaaa gccatgtcca cgcggtcaaa
      661 gcagtcaccc acagcgcggc tcgcccaaca cgtcgcagct ttgattcgcc cgctttcatc
      721 caccatctcc aacgatttca ccaaaaccac accgtgttgg tgtgcgaggt ccaaatccta
      781 ccaatctttt gccggtatgc caagggaggc caacccaatg ccaagccctg aaggattggt
      841 tggaaaaacc gcattgaagt aaccgattat gccccctgta accgtgtcag gctccctgcc
      901 ttctgccctc gtccagcctc gttcaagcaa gcggcagaaa ccaaaaccgt ccgtctgcat
      961 gctcataccg ccaaaactcc caagggcatc gctatcatcc gttgggccgg tgcttgcatc
     1021 acagccccgg caagcaccac acaacgctcc aattcccctt cccccttcac ctcgctcccc
     1081 tcccccacca cgcattctcg cagcctcacc cgcgggttca tcacggccga gggggcaacc
     1141 cacgacgata tcccccgttt tttcagccac ataaggttcg cccgcaagta ttgctccggt
     1201 gttccgatat ccacccactc gccttcgtgc cacgccaccc ccacccggcc ttgttgccga
     1261 agccacggca gcagagcatc tcccaccaaa cagccttccg gcgggagccg ttcacgtaaa
     1321 cgttccgtaa gcaccatcac cccaaggtaa tctccccctg cctcttcctc cgaaaatcgc
     1381 tctccccgca gccgcgccac cgcagcgacg cggctcactc ccactgtccc ctgccccgcg
     1441 gcacgacgag ccaccaacaa agttgcagcg gcttcgaggg tccggaccgc ctgggcgtga
     1501 cgtgcgagta gccctggcaa atccacatcc aattgcatat ctccgttcca gacgaggaca
     1561 tcgccgcgcc ccagcacgcc ttgggcgttg gccacccctc cggctgttcc caggatccgt
     1621 gtctcgtgta atacattgat ttcaaaaggt attttgtctt cgaaggcttc ggcccgatgg
     1681 tgtgtattca tcgcgatggg ccccacgccg aacgatcgaa gctgctcgat gatatgatga
     1741 aatgccggac ggtccccgat aggcagcaac ggtttgggga tatggtcggt caagggacgt
     1801 aatctggtac cgaatccagc gcataaaacc atggcagaca aagggggcat gattttttct
     1861 accacatcga ccgcgggctc cgaagcttct taccaaaaca gcccgtccct atcgttgccc
     1921 tccctatttt gccgcgccgg gcaggtcccc ttgctggttc ggcacggtgt gattggcatc
     1981 acaacaccgc ccgtaccgga ttggctctgt cgccatggcg aacgagatac gccttgatcc
     2041 gcgttcaggg gcgcaggttg cgagattggc tcatttgcgg taatgccagc gccatttcgt
     2101 ttgcgccgca gtgagataac cgtaatagag taagttggtg gaattgcccg catggagcgt
     2161 gattccgcgg taggcgccgc ttttgaatga ggcgtaccag gagtccggca gtcgaaccca
     2221 cttgccttcc ggttttccga aattgacttc caatgtttgg ttgattccgg ggaacgtgcc
     2281 gggcttggtg ctatggccat gaacaccgat acgagccacc cccccggaat tccaatacca
     2341 gtgggctgca taacaataca cccaaatatc cagtacttgc gcaccagcca ggtccgtaaa
     2401 catcttgtcc ggcgcaccaa tcgcggactt cagcacccca ttcgattcca gatactggcc
     2461 ttgataatta ttctgttgat tataattata ctggccgccg gaaccgagat aggactgatc
     2521 ccacttcctt gccgtatccg ccgatgtgta ttcgcacgtt ccgctgacgc agctaccgga
     2581 agggcatgcg cgcccgcagg agccacagtg atttttgttg gtggcgaggt ttacctcgca
     2641 gccgttttcg ctgttattat cacagttgcc atatccgcta ttgcagcttt tcaacgcgca
     2701 gacaccattc gtgcaagaag cagaagcatt ggaaaatgaa catgtttttc cgcatgtacc
     2761 acaatggctc aggctggttt tcagatccgt ttcgcaaccg ttgctcgcat tgctgtcgca
     2821 attgccatag ccggaaaggc attggaaccc gcatgttgcg ccggcacaga aggtcgtcgc
     2881 gtgggcgcgg tccggacata cattgccgca ttgctgacaa tggctcggat cggtattgag
     2941 atccgtttca caaccattgc tagcgtttcc atcgcaatcg gcgaatctcc cctggcatac
     3001 gaatccgcaa ttgccgcttt gacacgtgct ttgcgcgtga gccatcgtgg ggcaggcatg
     3061 ggtacaggtt ccacaatgac cggggtcggt atcgagattc gtttcacaac cgttggctgg
     3121 atttccgtcg caatcgtcaa agccgggcaa acaggaaacg agaatacatt caccgtaaaa
     3181 acaatcggtt acggcattgg ggagggaaca agtgcatccg cattggttgg caacaccagc
     3241 gcctccgcag gtgttgggcg gtttgcagtc tccacaatcc aacggaccgc aatcggtatc
     3301 gacaatgccg caggaagccc cgagttggga gcaggttttc gctgtgcagc cacattcgtt
     3361 ggcaatcccc ccgcccccac agacgtcggg agctttgcaa tccccgcaga atagaacatc
     3421 gctgcaacca tccgatatca gaccgcaggc agctccgagt tgggaacagg ttttggcggc
     3481 gcaatcggcg ccaccgcaaa gattcgggcc agctcctcca catatttgac caggatcaca
     3541 ttccccgcag ttcagcgttc catcacaacc atcgaggacc gtgccgcaat tgacgcccag
     3601 ttgagaacac gttttgggag tacaaccaca ttcattgttt tcaccgcccc caccgcaggt
     3661 ttgaggatac gcacaaccgc cgcaatcaat cgcctcccca cattgatccg aaacccaacc
     3721 gcattgagca ttcaattgca ggcaggtttt gggaatacac cgctcggttc cgcacgtatt
     3781 gggaccagca ccgccacaaa actgccccgc cggacaagtg ccgcattcca ctttgtcgcc
     3841 acaaccatcg ggaatcgaac cacaggtggc cccgacttgc acacagcttt tcggcttaca
     3901 cgcgaccgga ggcgctcctt ccggcaatac ctccgtttcc cccggagcat cgagcagctc
     3961 atcgccgctc tcttccccta cgctcgcgtc ttcgtcgtcc gctggctcgc tttctcctcc
     4021 acatccaagg aaggccaaaa aaagcgctgc aatcaacgcg acataaacag cctggccgtc
     4081 ccacccgcag ctccggaaaa agccgagcca ccaccgccgc gaatcggtcc ccaatttccc
     4141 attttcggcc atcgtctcac ctttttcccc agatgctgtc gccatccacc gggaatcccc
     4201 aggaatcccg agtgattatg ccccattttg catttcaatg caaccgtgtt tttacactcg
     4261 tactccttgg taacaagcgg tccgaccacg tcgagatgat ccgacaccag cctccctcct
     4321 cgccttttat ggctattccc cgtcttttcc agaccaagag ccccctaccc atacatccca
     4381 cccccctatt ttttccgcta caatgcgctc caacgcctcg cgcgtcacgc ccaatcgctc
     4441 cgccaacaaa tgggccagcg acgttttcgc aacccccggc acgaaacgat aggtaggttg
     4501 attgtctcca tccaactcca cctcgagaaa agccaacccc cgcaccggcc tttccctttc
     4561 caattgctgc gccattccca aataatgcgt gctgatgaaa acctgcggac gaagcctcgg
     4621 aagcagcgat atcaccaccc gcgcaatctc ctcgccttcc gtcggattcg ttccggaaca
     4681 taattcgtcg atcaacgcca tcgatgccgg ttgcagctcc tcaaaaagcc tacgaattct
     4741 aacgagttcc gtacctaacc gtccctcggt ttgcgccaca tccacctcat cgaccagaga
     4801 gacaaaaagg gtgggcgctc ggatcaatcg cgcccgcgac gccggcacga acacgccgcc
     4861 ctgccccagg cattgcacga gacccaccgc ctgcaaaagc cttgtttttc cgccggaatt
     4921 cgggcccgtg agcaatacca gcgcatcgtc gcaccccagc cgcacatcca ccggccgcgg
     4981 ctccaccccc tgcaattcca acaatggatt gaatacccct tccaacaccc gctcgatgac
     5041 ccggttttcc gatgggcagg cagcgtccac gatctccggc aggcaaaccg aaaacccacg
     5101 cctggtcgac cgatcccgaa atccaagccc cgccaaataa aactgcatcg gccccacaag
     5161 cgccaaacaa accaccaccg tatccaccaa cgacgaaaat acctcgtgaa aaagcgatac
     5221 cacgatctcc cgctgcccgt aacgatgccc ccgcaccacc gccatcagcc gctgccagac
     5281 acgttgccaa actgatggaa gtaatggatt ttgctcgttt tcttttacac tccgcaaggc
     5341 caattcccgc acatgaccat ccgcccccaa tcgcaatcga acatccaccg tcgcaagatt
     5401 cccctccaaa tccaataatt gcctcaaacg ctcgtagcct tgcgatccac gcatgcgctg
     5461 ccccgcttcc cgcaatctcg aaagcagcga cgttgcctgc ccaaaccctt ccgcaagagc
     5521 ctccacacac gcctgaaccg cgcgcaaaat ctcgagtttc ctcgctatcg gctccggttc
     5581 ctcttgcagg gtgctgtcga gatgcgagcg caaccgggcc accgcacgat acacccgctc
     5641 gagatccgag cgaatcactg gctgttccag cagttcggaa aaaacctcct gacgcacccg
     5701 tgtatctgcc ggattcgacg ggggatgcga taacgcccgc acaagaaatg cctcgtgaat
     5761 ccgatggggg ataccgtcaa tttctaaagg aaaacaggtt cttatgagat catcgagaaa
     5821 tagatgtccc gcaaacgttt ccgcatgcca gttcgaagga ctcaacggtg tagcccgtac
     5881 gagttcatcg aatatctccg tacactcacc cccctcgaag gcgaaacgaa caagccccgc
     5941 caccgacgcc agatcagtcc aagcctgcgt tttttcatgt aaaatatctg gaaccgccag
     6001 gttcgatccg cgaaacgggt cgtcggaggg gcattcctcc caaagctccc ctcgcccacc
     6061 agacgctcgc gctggcacgc cacgactcaa cgatagcgaa ttcttatcgc tcatgatctc
     6121 atcggcaact cgcatccccg attcatcgaa aacctcgggg ttccgaggga ccgccccatc
     6181 ccctcatggg acgtcgagcg tatacgatag attgttggct ttttcatgaa tgttttccac
     6241 caccaggaac atgggccccc cttcggtcaa aaaggcgtcg ataagagggt ttttatcctg
     6301 ggccccgcga tcgtcgttgc gcgcaatcca ggagcttatc gcatcggcat aaatcatcaa
     6361 tcggccgtcc atatcccctt tcgccttcaa atccaccacg atatccgcgc ccttcgaacc
     6421 cgtaaaggca tagaggcgaa gaccggaggg cgagccgagg gaatcggtaa aatgcgatgg
     6481 gaatacgaga ggttgccctt cgaaggccga agtcgcgtgt tccgtggctt gcagcgaata
     6541 ggtgaagtcc acccctccca ccgcaggaga agaaagattg cgaacatccc gcaccgctac
     6601 ataatagccg cccgaatcca tcgccaagaa ctcgagcgtc gcaggctttc cgactgccac
     6661 tttgacgtaa tcatcacccg gcgctttgtt gcctcgtcca tcatcgaata caacgagatg
     6721 tggttccagc gcggacccgg gcggcagcca caccaccacg cgaaccacct tgccagcctc
     6781 ggcttgcacc cgaaaaatgt cggcgtcccc caccgtgcct atgcttcccc gcatatccgc
     6841 atccaacggc aattcgttgt actcttcgag cgtcgcgcca tcgttgggct ccgtttcgct
     6901 gagttccacc agcgtcacgt ccgcctcggc aacgtcgttt tgaaccggag catcggagga
     6961 agcttccgac gacgcctccc ccacatcctc cgctggcccc ccctccccct gggaaggagt
     7021 atccgacgga gcatccgcgg aagaattcgt ctccgccaag gcatcctccg cgggagtaga
     7081 gtcaccgggc gcatcggtgc cggcatcggc gtgaacatcg gaaggcgacg atgtttgctc
     7141 gtcagtgccg caggcggcga gccataagga caaccccacc aggggaagac atggccagga
     7201 tctgttcatc ctattgaaat ccttatatta ttttgttgaa cgacccctgc gggggactga
     7261 tccgaagcat ggtttcgcac gatggatcgg gctcgtcaat cgatcattgg aggagttggg
     7321 gagcatcgcg acgattttcg gcgttggggt tcgagcgttc gaaaacgcgg ctacttcggt
     7381 gtgtggtcgt cgttcgaggc gtttccggag cagtctgcct cgagcctttt caagcctgcc
     7441 tgggctcttt gctgccctgg ctgcaggtga agggcaagac agaaagcatc ccgggcggag
     7501 tcatttcgtt gtaatttttc gagggattcg cccaacccca gggccgcggc cacggaaacg
     7561 gaatccaatt ccaggacttt ggcatagctt tccgccgctt ctgcccaacg cttcgaggcc
     7621 gccatcgcat ccgccaggcc gagccatccc gcggggtcct gcggcgcata acgtagcaat
     7681 aacacatagc ttttttctgc gtcccgcaga tttcctcttc gcgcctggac gcgacccagg
     7741 tcgagcagga tatctgaacg atagggcgcc caacgatgag ctttgcgcaa cagcgattcg
     7801 gcttcctccc agcgcccttg gcgggttctc agaattcccc atagatgcat cacgtcttca
     7861 tcccaggggt tatccgcctg gcttcgcgcc agcgcccgtt gtgctttttc ctcctccgat
     7921 agggcgatcc gcgccctggc caacccaatt cgggccccgt atagcgtgtc gtcgtagccc
     7981 agggcggttt cgaagtgccc cgctgccgcc cccgcctcgc ccgaagcgag taccgccgcc
     8041 cccaagttgt aatgaacgag agcggctcgc gggacgtcgt cgcgcaagga agatagcact
     8101 tcgatcgcct cttttcctcg cccaagaccg aggagccaat ggccgtaatc gagccgaaag
     8161 cgatagtctt ccgggcgcag cgccaacgct cgttcatagt agcgccccgc gtgggagggg
     8221 tgccccaaag ccgcgagttg cgtggcggct tcgaattgtc tatccgcatc atcgggataa
     8281 agcgccgcca aggcttcata ataggggagc gtggtagcaa tctgatgctc ataaaggggg
     8341 acgctatggt tcgacacgtc gagttcgcgc gctcggctca acagaccccc gaggacgagc
     8401 ccctgccctt ccgccgaccg gacgatagct tgtttttgat cgggagaagc tttcaccaac
     8461 caaggtgaaa tgggctcccg aaccgccaac aggcgagcgg gaaccatgcg acgccccagg
     8521 gaaaaggcgg ttcgatacgg aatcataggg cgatcatcgt gctgaacagg caatccccgc
     8581 gtccacgcct ccaaggaggc agggccggcg ataaataggt ttagaagttc ttccacgctt
     8641 tcgacccccc aatccgccaa gtcctttccg atggtggaat cacgcaattt cgctgcgatt
     8701 tgcgcgggat cgatcgcgag cggtcggccg cgaatcccca ccaacttggc gtatcccacc
     8761 tggccgtagg tttcgaaacc cacattggcc cataaggtca tagaagggaa agcctgctga
     8821 aaggtacgca cgatcatctt gaattcgcgc tccgacagac catgcagcgg gagccattgc
     8881 accacgattc cgccttccga aagcctgtct cgcaccaatt cataaaactc cacggtatat
     8941 aaaatccatg aatccacggc cttgggatgg gtggagtcga tcatggccac atcgaaacgc
     9001 tccgacgtgt tgagaaggta ttgccttcca tcatcgatat gcagatgaaa acggggatca
     9061 tcgagaacgc cgcgattttg cttttcgaag tacctggctc cgcgagccac ggcggacgat
     9121 aaatccacga cgtcgaggct atcgagggga tgggcaagtg cggcgcccgc ggaaattccc
     9181 gcgcccagac aaatcatgac cccttttttg ggattcggat gcaataaagg cgctagatga
     9241 ccgagcactt tgaaactctg gtcgtgaacc agacgcgtcg ttacttcgtt gattccgttc
     9301 atcaacaact gccgttcctg attgatgcgg ttttccacga cggagacggt tcccgttctc
     9361 gcttcctcgt aatgtaatag cgtttgatgg cgaggaccga cggtagccag cagcatggcg
     9421 cggggcatcg cggaggggat ccaaagcgcc aaaaggccca cggcagcgat ggggagcagg
     9481 gcccaacccg cggttcgaaa gcagcgggga cgggtgaaaa agagcacgac ggcggccatc
     9541 agagcactca ggatggccag ggcgaaaaaa gcgccttgta cgctgaaaac cgttccgagc
     9601 aagagggaag tggtgatggc tcccagagcg gaaccgagac cattgacgaa ggtgacgcgg
     9661 cccaaaccgc gaccgacgct ttcggagcga tgggcataca agctacaagc ggcagggaaa
     9721 gcggctccag ccaacagaca aggcaggaaa agcagcagtg gtgtgaaggt aagttcgcga
     9781 aatatttcaa taaaaacgga gcccgcgaga tagggggaag attcgaggcg aaacgggtcg
     9841 cggccgaaat ggatgacgag atgagccgcc aaccaaaagg aaacgaggga ggcgacggag
     9901 aagccgccca acaagccagc ggtccaagcg gcctgatccg cggaggcgac gagcctgtgc
     9961 aacgcgcccc caagcgccat tcctacgaga accacggcaa gcatggtggc aaaagcgtag
    10021 gtgtcgtgtc cgaacacgaa ggtgagcaca cgggtccaga gcacttcgtt ggcgagcacg
    10081 acgaacccca tgatgaacgt ggcgagcccg gcgagacgtt ggaccgtggt gggggtagtc
    10141 tggggaagga tttcagggga gagggagtct ggaggtgggg tgctggggcg aaatgactcc
    10201 gcgagcgttg cgagaaaagc ggcggaaaag ctacccaagg ccgccaatgc ggaccaacgg
    10261 gcaaccccga gcagcgccag cccccaatag ccggtgatcc acgcgcccat ggctgctcct
    10321 gccgtattgg ccgcgtacgc caaccccacc gtccaccgca gggttcgtcc ttttcgtgtc
    10381 cacgcacgga cgagcaaggg caacgacatt cccatcgcaa ggcagggcag agcgatgaga
    10441 agcaaagcca gcaaacgtcg cagcagcgct cccgattcac cggggatttg cgataaagaa
    10501 gagagcagcg gcggggccac gacagcgtat gcccccagca gaagttccat cagggcgtag
    10561 atgcggaggg gaaaacgggt gcgttccgcc cattccccgc cgatccaagc gcctgcgcca
    10621 atgccgatca tgaaggcagc gagaacgatc gccgcggcat cggaggcatt gcctaaaggc
    10681 acgacgagcg ctcgggacca ggcgacttcg taagcgaggg aagcgccacc ggagaaaagc
    10741 gcggcaacga tgatcggtgc cctgcgaaaa ggccatcgac gaaaagcggc gaacacgggc
    10801 ggcaccatag caaggcccgg cgcgcaagag actaggaatc caccagaaaa ggcaagtgga
    10861 agcgggtcgg gctcaggggg gcgcgaaagc aggggctcgc cttcagccag gcagcgagca
    10921 tgggtttcca gttcttggta gaagtacgag gcagagggtg atgagaaggg ggatgagtgc
    10981 ggtcacgttc tatcgcgcat acgtttttcc ataatttcgg cggctttaat caaaggatat
    11041 cccgcggggg aggcagtcaa gcaaggatgg gtgccgattt gggcgatgag cagagagttg
    11101 acggtcattc ggttttggat gatcaagccg atgacattcg tcaattcgcc cgcgctcggc
    11161 ccaccgatga cttcaccgcc gatgatgacg ccggagtttc tggcggcaat gagtttgacg
    11221 gtttgttttt gaacgccggg gagggtgcca gggtggcggt cgataccttc gaatatgccg
    11281 gtcacgacat cgatttgttc tgctttggct cgtgcctcgg tcaatccggc ggtgccgaat
    11341 cccacgtcgc cgatggcggt ggcgtagatg gcgatggtcc cggagaaggt tttgacggta
    11401 tgcaattgat aaagattgcg gccggcgatt cgagcttcgg cacaagcggt ggaagccagc
    11461 atcgtgggca cgcggcggcg cgtgacgaaa tcccgttttt gtgcgcaatc tcccacggca
    11521 aagacatcgg gggtgtgggt acgcatatat tcatcgacat cgatgaagtt gttttcatcg
    11581 atgtcgatgc cggcgtcttt ggccagtttg gagtttggct catagcccac ggaaaccacg
    11641 actgcatcgg cggggatgac gtcgccattg tcgagggtga cggattccac ctttgtttcg
    11701 cctccgattt tggccacccc tacgccggtt ttgacttcga ttccgcgttg ttggagcagt
    11761 gtggtcattt tatcggagag ttccacatcg aaggcgaggg caaggatatg gggcagtttt
    11821 tcgatgaggg tgacgtggcc tccggttttt ttgaattcgt cggatagttc cacaccaatg
    11881 aagcccgcgc ccaccaccac cacgtgctta caggcggaga gcttggcttt catctggtcg
    11941 aggtattctt tttctttggg aacgacaaat acgttctctt tttcacggcc ttcgagccat
    12001 ttgggcagga tgggagtcga gccggtggcc aataccaatt tgtcgaaacc gatcgtggaa
    12061 ccatcggcaa aagtagcgac tttcttggga atatcgatgg agacgacttc tcccaccacg
    12121 ctttcgacac cggcctggct catcatcttg tcgacaggca tgatgttctg ctcacttccc
    12181 gccaacgtgc cgaagatata gggtattcca cagggcacca tgacgacgtc ttgctttttg
    12241 accatcgtga atcgtttatc gggataggca cctttgccgc tgagagcgac caccattccc
    12301 gccgcgcttc caccaatgac gagtacatcc gtcgatttca tgttcactgc ctttcctgga
    12361 caagcaccga aggtgcgtcg aattcgtatg tttcggcttt cgccgagacc tgaaggaatc
    12421 aggcagcccc tatctaatgc gcggcaaagg gtttggacag tcctttggag gttagaaaat
    12481 atttatgatg taggtatgtt gcacgtttga aacgagacac tgattcatcc gtgccaaaca
    12541 ccacaataag ctcaaaagga taatggcgca tggcggaaaa cggtcctcgg ccggcgcttt
    12601 gattttggta aatttatgtt gaattacgaa gggttatatc attcgcgggg atcgaacggc
    12661 gcccatgtac ggtgttgacg aatcagtgcg ccgtcggcgt gttcgagcat gaggcgcagc
    12721 aacggttggc gcaagtcgag gggaatgagg tcgaaaggag cgtgtagcgt atcgaaacga
    12781 agcttcatca cttctggatg agggcgtgcg cgtcgtctcg atggttgcgg cagtttgccg
    12841 cgttgggtca gaagccacaa cagatgttcg gaggttggcg catcctggtg cagggattgt
    12901 gatgccagcg ccgccatggt ttgttcgatt tgagccgcgg tataaccaca gcggccgggg
    12961 tctgccccga tcgcttcgag catacccacg acccggcggc acttttcgca gcggccgcaa
    13021 ggcagtgctt tttcctcatc gagatgagcc gcatggcagg agacttgcca tgcctggagt
    13081 tgcggatagc gttgcgcgag ggttttttgg acgagcaatt cggaaagggg tctgagtatg
    13141 gaaaactgtt ttatccccca ccttttttta tcataatact tgctcaaggc ggcgtcgaac
    13201 caatggcttt gatcgtagag gccaccataa tgggacaatc cgtgaaagga tagtcggagg
    13261 gatgtgtcgt attcgttgcc gatcaagagc cttcctattg ctcgtgcgcg gagcaacggc
    13321 agcgatccca cgatcaatcc cgcgacggtc cacaatcgaa tggggtattg atcggctcgc
    13381 acgtgggcga agtcctgtcg aatgaaaggc agatgacgaa gcatccagtt atagacacgg
    13441 tcgatgttcg tccagacgcg ctgcgtggtg ggcacggtat ttttgaatcc gcgatgggcg
    13501 ttcagagcgg taaaccaatg ccgtcccgac tcgttgatat agatgggatg aacctcgaag
    13561 ccgagttccc gcatgagtcc gaaggtcaac agggactctt ttcctccact ggaaagcacc
    13621 gcgacacggt gattgtcatg ctcccatggt gcgacgatcc gcgagttgga caagcgcggt
    13681 gaaagcagcg gggcaccctc gagggaaggg atatcgcttg cgttgatttc gatgcgccct
    13741 ggcagcaaaa aaggattggg ctgacagagt ttatgcacga ggatttcgcg gttcgtattc
    13801 cgaagcattc cgcgcaatag cgccagatcc acggtatcga cggggccatg gatgacgatc
    13861 tgatcgcaaa ataggccgta gttgagcgcg gcctgcgcca tcatcatggt cccgaggttg
    13921 tgatccgcgg catcatcggc gtcgaatacg tcttgttcgt accgatattg aagggtgatt
    13981 tcgtcttttt ggcttccgtg gaacaccgca taaggagccg agagggctcg gggttcgagc
    14041 gaaacggggc ccacttccag gcgatcgaag ccacgaaggc tcgtcatcgc gggtggcatg
    14101 tcgattccca cgaacgttgg tgtgttcgaa gaagcgtgat gagtcggtcc actccctgaa
    14161 cgagcggtgc catggtgggt cggcccgtct gtttttcgat ttgttcgcag atcgaaggga
    14221 tgtcattcgc agggatttgc tcggtattga gggtgatggc cacgaccggt cgttgcgaaa
    14281 tcaactcgat cgcctggcat tggagcgcga tcggatgcaa ggggaagtcg tcgaagccat
    14341 cataggtttg gcgacctgga gcatgttgta ctacgacgac atgcgggcgg gtggcggcga
    14401 gcaattcgaa tccaccggga taagcgggat gaaggagact gccctgccct tcgaggacga
    14461 tcacatcggg gtgagcctgt ttccacgcac gataaacagc attttccagt tcgcccgcca
    14521 cgaagtcatt gacgatcgaa tcgaggatga tgccgtattc ggccccttgc aaccaggcgg
    14581 tttgtcctgt tccgacgagt tgcgcggaaa cgttggcttg gcgaaaggca tcgacgatcg
    14641 cccaggcggt cgtacgtttt cctaccgcgg aatccgttcc caatacggcg acacgaaagc
    14701 tatcgacttg tttgatgagt cccgaaaaaa agtgcaattg gtctcgatcc ggcggacgtc
    14761 ggatatcgat gatgcgagcg tgatgttggg cagccaacga agcgagagcc gggtcgtcgt
    14821 tcaaccaatc atgcaatccg ttatagacat cgaggccttc gttcagagcc acggcaacgt
    14881 catgaagcgc cgaagcgggg aggcgtccac catccggagc aagaccgatg atcaaggcag
    14941 aagcggcccg atcggctcgt cgagaggcct ccagcgcgct ttggaaattt tcgaagacga
    15001 tgatatccgg attcgctccg tcgacgacat catgggcgtg gcatccggcg caggatgaat
    15061 cgacaacgcc cacgatctga aagcgttcgc tgcgtcggac gagaccgtgg gccgtcttgc
    15121 cgtgggtggt acgaaaagcg ctttcgcaat acacgatggc gcggggtttc atcctttcct
    15181 ccctgtgagc acaacgacat aacgatccga ggggaggttg gattcgcggt gcaactcgat
    15241 gagcgcatga aggccggcgg tggcggcggg gagcacatcg agtccttccg cgcttcgaag
    15301 ggatttggaa agagtgctca tttccttgtc gctggcatca ttggcgatgc cgtgggtagc
    15361 gcgaagcgct tcgagtgccg cttccccatc gacggcatgc caattgacga gcggttcatt
    15421 gactttggtt tcgcgaatgg acttgggccg tagatcgtcg caacggagca agttatgacg
    15481 aaaagcgtag acgattgggt ttttgttgtg ggaggagccc gcgatcatgc gtggaatgcg
    15541 ggaggttttt ccacgacgat acaagctgag gaagccgcgg tacaacccag ctagtgtggt
    15601 gccattggag acggagacgg cgacagcggc gggagcatca cgcagttcgt cgtagatctc
    15661 gtaggcgatg gaaccatagg ccatgagttg cagggcggtg ttgtcgcctc ctgggttggc
    15721 atcgtaaagc tcatctttcc gggcgtgttg gcgtgacaac tcgacagcgt tttcgtaatc
    15781 tccatttgtc cggataagga tagcgcccgt gcgttcgatt tccgcgatgc gcctggttcg
    15841 gtaagaggca ggtatgtaga cgtaacatcg aaggcctgcc agcgatgcgg cgagcccggc
    15901 ggcggcaccg taattgccgc aggtggcaag agtc
//
LOCUS       DAOSOR010000052        15752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_3748_length_15752_cov_3.775307, whole genome shotgun sequence.
ACCESSION   DAOSOR010000052 DAOSOR010000000
VERSION     DAOSOR010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 15752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15752
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3748_length_15752_cov_3.775307"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1710
                     /locus_tag="PK710_04545"
     CDS             <1..1710
                     /locus_tag="PK710_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09014.1"
                     /translation="RLAKLGSGFASTMRLFSMMQRLFVACRLTLFVLILGAGACDPSS
                     TLRDKPSALVAESHFHPDLDIDQRACHVRRNALLSQPDVGGTPAYDAMRAEILGRAWG
                     EPMVFMREPTRLPIQQLSASTRTLAIAFDRAPHGVRVDRLLRQARNQPALLRSILLPE
                     GYVYASDPLDALEVVRSLQLPLLFQEPVIWLQRQAEVVRLERFRERNTSLYRYAEGPY
                     QGQPASLQFGDRLAISPAEFANPLHRDLRTLARDVGFDRVRIEHRVASALVVQLRFGT
                     RWVRGVLTAQGASLQLACLDEPRATVEWVEAWMRENGHQIRARRAMEAVIDTEVRDAL
                     PFDRPIDAEDHFSDGALRPLWFDAYRRGQTMFSHQGRGYPVYRSDGIAWPPQVCVDLV
                     LETYERTSGTWYRPRGQPPERIVGRLDFDAEGITNRRGVLAFGTFAESSPSLFEYEKL
                     EERNRIPFAKRKEFFAYLLEHADDFGPGHVVAIQGKKRDGHIHQHAIFVETTDPVTGF
                     PYGLFDQMKKPRRRTWEGIMAEAPARSLYYHVRPTDKIWRAMDPQPGQIMPSPLRRLV
                     QSR"
     gene            complement(1731..2369)
                     /locus_tag="PK710_04550"
     CDS             complement(1731..2369)
                     /locus_tag="PK710_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09015.1"
                     /translation="MAIRWEAGVLVLAALGSGCSDAQGEFDDFVKRSATIKGDDGGKD
                     VAVDAGDCPKVTQQDVANGYYFTLSAVIVPKKPFVVLGTVTVDEKANTVSFHFQPLSK
                     DDKSTPVGSPIVGGPFPIADDGSFEADFGSISMDGAANPISGSELATTLVLKGEAGGW
                     CGSSEFICGTVEGIVSKPLKDLNLKGSTFTMQKLTSAGSYPTPIINCAGDTP"
     gene            2541..3920
                     /locus_tag="PK710_04555"
     CDS             2541..3920
                     /locus_tag="PK710_04555"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOT09016.1"
                     /translation="MQHSTRFSISVLTTIGCALLAGQAQASGFSTARFGGEHGHPMTT
                     NPTAVYYNPAGIAKSEGFHLFIDTSLAIRSASYTHSAARTDLPEPDDATGANTDEAEL
                     FNVLAVPMLGASAKFGDFALGAGYYVPFGGTSIWEKNEKFKNNPTYPGPYDGANRWYS
                     LNGTISSTYYTLAAAYHIPDTGLHIGLSGNLIRSVVDTIRARNADGSNDVALEGRSYA
                     DVSGWQVGFGAGVLYDVLPQMLTLGVSYQSRPNVSGGMILKGTLQTKLATSEPTIQDV
                     ELHQDLPDVVRFGVQVRPLRDWEFRGFMDLSRWSAFKQQCLGEVGKPCELNDDGSPAE
                     GSDVRQNIPRHWKDAWGAKVGASYYVNEPLELMFGLGYDGNAIPDQALDPALMDFHDL
                     SATIGGRYHLLDPLFVALNVSQIFYFSRDTNGKSTNARWQVPSRSPDAGGKYEQAITV
                     INANLEMVF"
     gene            4327..5967
                     /locus_tag="PK710_04560"
     CDS             4327..5967
                     /locus_tag="PK710_04560"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT09017.1"
                     /translation="MRKQGHGSRQAAKKKNLVLAFPPYTLPMSPPLGVCSLAGYIRRT
                     LPDWNVRVFDFNLDWHRLFFDSLGPSRRPSSLTIDDTNLRYAAELFHTGLAEDFHQRA
                     RYLHFAQVWGLAMRQPRFLRKNLEPAFLSGKMPDYIRHQADRVLEENPDIVGISVCYA
                     LQRWPALCLAREIAQRSSIPLVMGGTVFSDGVPKGWGAGHRVADYVVMGAGEQPLAAI
                     LEGKVNDGPIPGVVRIVDGKASSAAPIHESDLDSLGTPDFSDLPLDRYYSPQPVLPIT
                     TSRGCYWRRCAFCSHFQSSGQVYQLRSIEAVIDELREHVMLGVRHFYLADSVVSPSRF
                     RRFAQAIVDSGLKIHYYAMARAEKAFDRELLDLISRSGCRFILWGLESGNQRVLDLMD
                     KGISAETTETILQTSREVGLRNHVFVICGFPTETRQEFQDTIDFLVKNKENIDVVHRA
                     IFSMEENSLVHRFPEKFSINDFWPKATPRLFDFTTTKGMTRAEVKAAFLAARPTMLSF
                     SGHPSVPGDFKFREHCLLHYAKNSEEPPKHESPSMRLA"
     gene            complement(6016..7572)
                     /gene="norR"
                     /locus_tag="PK710_04565"
     CDS             complement(6016..7572)
                     /gene="norR"
                     /locus_tag="PK710_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HOT09018.1"
                     /translation="MNQLDAIISIASDLTAGLSSEERYRRLLAAIRNVVPYDAAALLR
                     LEDTSLVPIVAEGLSPDALGRRFALDEQPRLRLLCEATEPVIFPVDSELSDPYDGLLA
                     SDLGDFQRIHACLGCPLRVRGELLGVLTADAAQPHAFDHIDRSFLAAVGALAAAELRT
                     TQLIEALERSADRQGRIARDLMRDVELRQGSDMIGTSAVMQRLRQDIELVARSDLTVL
                     ITGETGVGKELVARAVHNASMRHAEPMLYVNCAALPETLADSELFGHVKGSFTGASSD
                     RMGKFEVANGGTLFLDEIGELPLSVQPKLLRAIQQGEIQRVGSDKAIKADVRILAATN
                     RDLEREVQANRFRADLYHRLNVYPLSVPALRERAEDIPLLAGYFCDLMRRRLGLGPVR
                     LEPEAVVALQRYPWPGNVRELENVLSRVTLNAASGVSRTDPVVIRGFHLGPDFAASRV
                     TTETDLEAGVTKASASMTLHQATRDFQRTLIARTLQEHHGNWSATARHLGMHRSNLHH
                     LAIRLGMKST"
     gene            7732..8394
                     /gene="ytfE"
                     /locus_tag="PK710_04570"
     CDS             7732..8394
                     /gene="ytfE"
                     /locus_tag="PK710_04570"
                     /inference="COORDINATES: protein motif:HMM:NF008221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HOT09019.1"
                     /translation="MKNTERSLGELVTTFPMAAKVFQQYQLDYCCGGKQSLADACAKE
                     SLDPETVLTAIEAASRDKTDETRWDQRPLPELVQHILDRYHAPLRTELPRLIGLATKV
                     EAAHAEKPTCPKGLTELLTRVARDVENHLAKEEQILFPLIVAGRGRTAHMPVQVMMRE
                     HDDHGEDLRAIREITNQRKVPEDACATWRELYRALGEFETELMDHIHLENNILFPRAL
                     AS"
     gene            complement(8494..9723)
                     /locus_tag="PK710_04575"
     CDS             complement(8494..9723)
                     /locus_tag="PK710_04575"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOT09020.1"
                     /translation="MRWSGVALFIITYGLISARRLQWLRFDRPAGALVGAVAFVVFGI
                     LTPRQALDSVDGATLLLLFGVMGMGAFLSVHGFFDRAEEGLVRLARTPPRLLGLIVWT
                     AGPLSALITNDAVCILGAPLVVRIIQKHRLTPLPFLLALATASNTGSVATLVGNPQNM
                     LCGLLGGLTYRHHVLLMAPVAAAGLLINHGILRWLFRKTLPKEPFAKSETRSEGSWHS
                     HVTLGVIAATTVVYTLGGDLAWTATAGFAVLMLIHRTDTQHLWKRIDWSLLLFFASLF
                     VVVEGLNRSGAPAWFFQQYPLGGRQEGMLEWFRLSAIFLVGSNIVSNVPFILVIREAM
                     GSLANPIMGWELLAMASTFAGNLTLLGSVANIIVAEASRDIGGIGFWQYLRVGFPLTL
                     LSTLVGTVWMALIGPGW"
     gene            10168..10290
                     /locus_tag="PK710_04580"
     CDS             10168..10290
                     /locus_tag="PK710_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09021.1"
                     /translation="MAVEEMAYDDFAWDLEEDERPTKRWQPFRPAAPTLRCRFA"
     gene            10431..12209
                     /locus_tag="PK710_04585"
     CDS             10431..12209
                     /locus_tag="PK710_04585"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOT09022.1"
                     /translation="MRNTIVQRTVLSRMALATTLLIALSHGACGSEETSGPSGGPVIG
                     TGGGSTGGVRNPTCAKGTSAGEVQAPEFVMNLRGQTSWFASPIVYDLDGDGRNELIAA
                     YYSVYVFDEQGKELDRVKAGDGRIYAPHVVADLDGDGITEVVFGNDHQVFAMEWRDGK
                     LVMKSGWPVDTTTAGNAPEVRGLAAGDLDGDGFIEVVATTTQTASTEKGGAQVFVFRA
                     DGTLHQPAGIEWKAWPRYNAAAGKGNDADRNGQGHHGYGCFGLNVGIGNIDDDDDLEI
                     LVTYDNHHIQAFDHDGVALDASPWFTNRGSDHAGERLTWGQFIRWADPEVEKNHYHDH
                     TGDWPHPSWAEWLQWTASPPNVVDLDGDGKNEVLGVPNVEMHEPYETQAYAVMVLQGA
                     YGDGTRSAMRKEGWDVLPRGNRPISVKGYYPPGGIPSPTTVNLQGDDRPEIVVSLNDG
                     FIHAFDSEAKEIWRYNYTFGKAVMVATEVMVVDLNQDGSPECIFATYGAPDVLDSGHL
                     VILGADGTELHTVALPNPGKNGNGNGAPAAPTVGDLDGDGQLEIFVQTFEHGMDVFTV
                     PGSSTHCMLWPTARGGPLRMGQPNGH"
     gene            12297..12719
                     /locus_tag="PK710_04590"
     CDS             12297..12719
                     /locus_tag="PK710_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09023.1"
                     /translation="MDKLKLKPLGAFLLVGGLVLLLYTFRMDGPPWQARRSVQGEVLE
                     RYTSPSRNVAEPDLILRIRVWQGDKPRVLERKVREETWNAHPQRSQIMLTLQGEEWQK
                     ANVQGDSREHWISWIKIVGGALGAILGVVVLAYDRWWK"
     gene            12819..15317
                     /locus_tag="PK710_04595"
     CDS             12819..15317
                     /locus_tag="PK710_04595"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT09024.1"
                     /translation="MRSNREVPAEQVTLRTDPETLTDELQAVGDRFELLPEHTRKALT
                     LGLSLRNPGFHVYVACDPDLTFDKDILRAAEAAAQALPAPNDVVFVHDFERPESPKPL
                     ILPAGRGRELALEVDKVIDELAYRLLHLGAMPIVREAEEALAKELSSKNKQVVSDLES
                     FAKTLGFGVKAVPGGVQTFPILHGKPVSPEQFEVLDESTKRALADAEQKLSAAVEESA
                     KRIHEMTDNVNAASDDAQQKAAEQVIGTSIEPLLQSFRGIEPVVAYLERIREELARDW
                     RDFTEPTNEADEQQESRADEVDRGNPEVAKRTGRFRVNVFVSHRPDAKAPVVDAHTPS
                     FPNLFGYLERRARFGALLTDFLRIRSGAVAHASGGTLLVRAADLLADPLIWERFKRVI
                     RDRELTIEDPIGPVSLYATTLRPQPIPLNVRVLLIGSQHLYDQLMQLDPDFSTLFRVK
                     VEVGWWVERTLDNQRKLDSYLMSLPTVKQTEASFSRGARARLLDFATRLSGDRERLAL
                     TVLPLEETACFAALRAAERGDGLVTDDDIETAWHERRERSGSIEREVRDLAIRGEFLV
                     DTSGTRVGVINGLSVLTAGDVEFGQPMRITAVVSIGRDGVIDVEREAQLGGSLHTKGV
                     AIVRGFLSLLFGQERPLSLRVQIAFEQSYGEVDGDSASSSELFAILSALADVGIDQGI
                     AVTGSVNQLGDIQAIGGVCAKIEGFFDVCKARGLTGAQGVMIPKTNLRHLVLRPDVAK
                     AIADGQFHLYAISSVSEGIEVLTGLQAGSRDASGRFPAGSVFGRVERRLIELAELLRD
                     AEAGPVDRGAIADTSDPDNVDAGVDFQKRFDGDW"
     gene            15354..>15752
                     /locus_tag="PK710_04600"
     CDS             15354..>15752
                     /locus_tag="PK710_04600"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOT09025.1"
                     /translation="MNKSTSLPPPRFQVFVGFHTDVGRQRQKNEDALVEVGVPLGYLL
                     AVADGMGGHDYGDRAAIMALNTIQESLRESTDQPQESLAKSLRKANAVVFKEAEEVGR
                     TMGATCVAGLISGGKLYVAHVGDARAYLLRA"
BASE COUNT         3394 a         4395 c         4611 g         3352 t
ORIGIN      
        1 cgtctcgcca agcttggctc cggattcgcc agcaccatgc gcttgttttc aatgatgcaa
       61 cgcctgttcg tcgcatgccg tctaacccta ttcgtactca ttctcggcgc aggcgcctgc
      121 gacccctctt cgactctgcg cgacaaaccg tcagccctcg tcgcggagtc ccatttccat
      181 ccggatctgg atatcgatca gcgcgcgtgc cacgtgcgcc gcaacgcttt gctctcccaa
      241 ccggatgtgg ggggaacccc cgcctatgat gcgatgcgag cagaaatact cggaagagcg
      301 tggggagaac ccatggtgtt catgagagaa cccacgcggc tgcccatcca gcagctttct
      361 gcttcgaccc gtactcttgc catcgccttc gatcgagcac cccacggagt tcgcgtggac
      421 cggttacttc ggcaagctcg aaaccaaccc gctctgcttc gctctatttt gttaccggaa
      481 ggatatgtct atgcatcgga cccgctcgat gccctcgaag tcgttcgctc cttgcaactg
      541 ccccttctgt ttcaagaacc ggtgatctgg cttcagcgac aagccgaggt ggttcgtctc
      601 gaacgctttc gcgagcgcaa taccagcctg tatcgatacg ccgaaggtcc ctaccagggg
      661 cagccagcct ccctgcagtt cggagatcga ttggccatat caccggcgga atttgccaat
      721 ccactgcatc gggacctgcg aaccttggcg cgagacgttg gcttcgaccg tgtccgcatc
      781 gagcatcgcg tggcttccgc tctcgtggtg caactgcgct ttggaacacg ttgggttcga
      841 ggcgtgctca ccgcgcaggg cgcctcgcta caactcgcct gcctcgacga accccgggcc
      901 accgtcgaat gggtcgaggc ttggatgcga gaaaacgggc accagattcg agcccgacga
      961 gccatggaag ccgtgatcga cacagaagtg cgtgatgcgc ttcccttcga ccggcccatc
     1021 gacgccgagg accacttttc ggatggtgcc ttacgcccct tatggttcga cgcctatcgt
     1081 cgaggccaaa cgatgttcag ccatcagggg cgaggctatc cggtgtatcg gagcgacggc
     1141 atcgcctggc ctcctcaggt ttgtgtggat cttgtgctcg agacgtatga aagaaccagt
     1201 ggcacgtggt accgacctcg cggacagcca cccgagcgca ttgtgggaag actcgatttc
     1261 gatgcggaag ggatcacgaa tcgaagggga gtgctcgctt tcggtacctt cgcggaatcc
     1321 tcccccagcc ttttcgaata cgaaaaactc gaggagcgaa accgaattcc tttcgccaaa
     1381 cgaaaagagt tctttgccta cttgctcgag cacgccgacg atttcggtcc ggggcatgtc
     1441 gttgccatcc aggggaaaaa gcgcgatggg cacattcacc aacatgcgat tttcgtcgag
     1501 acgaccgatc cggtcaccgg ctttccttac ggattattcg atcaaatgaa aaagccaagg
     1561 cgacgcacct gggaagggat catggccgag gcccccgctc gcagcttgta ttatcacgtg
     1621 cgtcctaccg ataagatttg gcgagcgatg gatccgcagc cgggccagat catgcccagc
     1681 cccttgcgaa ggcttgtgca aagtcgttga agaaacgata ggatgccccc tcagggcgtg
     1741 tctcccgcgc aattgatgat gggagtggga taggaacctg cggaagtaag tttttgcatc
     1801 gtgaaggtgg aacctttcaa attcaggtcc ttgagaggct tactcacgat cccctccacc
     1861 gttccgcaaa tgaactccga agaaccacac catccacccg cttctccctt gagaacgagc
     1921 gtcgtcgcca attcactgcc ggaaatgggg tttgccgccc catccatcga aatcgaacca
     1981 aaatcagctt caaagctgcc gtcgtccgcg atcggaaacg gcccccccac gatcggcgag
     2041 cccaccggtg tgctcttgtc atccttgctc aacggttgaa aatggaagga caccgtgttg
     2101 gctttttcgt ccaccgtcac cgtacccaat accacgaacg gttttttcgg gacgatgact
     2161 gcggataagg taaaatagta cccgttggcc acatcctgct gcgtgacttt ggggcagtcg
     2221 ccagcatcta cggccacatc tttcccgcca tcgtccccct tgatggttgc cgagcgtttt
     2281 acgaaatcgt cgaattctcc ttgcgcatcc gagcacccgg agccgagggc tgcaaggaca
     2341 agcactcctg cttcccaccg aattgccata atttttcgca taacgaataa accattcatc
     2401 gtcacgtttt ctccaattga cagcaccctt ctgttggctt tatgacctgt tacgcgtttt
     2461 acgtcaacct gttcacgatg tagcttctcg attcttcagg ttgatcagga acatttttct
     2521 ctggggctga cacgactacc atgcaacatt cgacacggtt ctccatttcg gtcctcacaa
     2581 cgatcggttg cgcgttgttg gcaggacaag cgcaagcgtc tgggttttcc accgctcgtt
     2641 tcggcggcga gcatgggcat cccatgacga caaaccccac ggccgtgtat tacaatccgg
     2701 cgggaatagc aaaaagtgaa ggatttcacc tatttataga cacatccttg gcgattcgaa
     2761 gcgcatcgta cacccattcc gccgcccgca ctgacttacc ggaacccgat gatgccacgg
     2821 gggccaacac cgacgaggcc gagcttttca acgtgctggc ggttcccatg cttggggcct
     2881 ccgccaaatt cggcgacttc gccctcggcg ccggctacta tgttcccttc ggcggaacgt
     2941 ccatttggga aaaaaatgag aaattcaaaa acaatcctac ctatccaggc ccttacgatg
     3001 gtgccaatcg ctggtattcg ttgaacggaa ccatctcgtc gacctattat accctcgccg
     3061 ccgcctacca tatccccgat accggattgc atattgggtt gtccggcaac ttgattcgtt
     3121 ccgtggtgga tacgattcgc gcgcgcaacg ccgacggttc gaacgatgtc gcgctcgaag
     3181 ggcgctccta cgcggatgtg agcggttggc aagtcggctt tggcgcgggt gtgttatatg
     3241 atgttcttcc tcagatgttg acccttggcg tatcgtatca atcgcgcccc aatgtttcag
     3301 gggggatgat tctcaaaggt acgctgcaga ccaaactcgc caccagcgag cccaccatcc
     3361 aagatgtgga actgcaccag gatctgccgg atgttgtgcg attcggcgtg caagtgcggc
     3421 cacttcgcga ttgggagttc cgcggcttca tggatctatc gcgctggagt gcgttcaaac
     3481 aacaatgtct cggcgaagtc ggcaagcctt gtgaactcaa cgacgatggc agccccgcgg
     3541 aaggcagcga tgtaagacag aatatccctc gtcattggaa agacgcctgg ggagccaaag
     3601 tcggcgccag ctactatgtc aatgaacccc tggaactcat gttcggcctt ggatacgatg
     3661 gaaatgccat cccggaccag gccctagacc ccgcattgat ggattttcac gatctttccg
     3721 cgacgatcgg cggacgctac catctgttgg atcccctgtt cgtggccctg aacgtgagcc
     3781 agatctttta tttctccaga gataccaacg gcaaaagcac caacgcccga tggcaagtcc
     3841 cttcccgaag ccccgatgca ggcggaaaat acgaacaagc catcaccgtc atcaacgcca
     3901 acctcgagat ggttttctaa tcccccctga gccatcccct cattgttcgc cggtcctttc
     3961 tttcacggcc cacggtccac gcaaaccggt cacggtcacg gtcacggtca cggtcacggc
     4021 ccacggccca cggcgcacgg cacacaaaac cggtcacggt cacggtcacg gcccacggcg
     4081 cacagaacaa gaaggcttgc tagcccgcga gcatcaccgc ccagcgctca aacaaccgta
     4141 ttcattgatg ttttacaagg ttgttcccaa tggcctcgag gatcaggtcg aagtcgaagt
     4201 ccagcggatt ccctgctcgt atccctttcc aatgcaagaa aaaatgaggg ggtctgccag
     4261 gtccggaata aatgcttccc gggatgggtt tgactttgaa tgacttttgt tgacacttgg
     4321 tgaatcatgc gaaaacaagg ccacggttct cgacaggccg cgaaaaaaaa gaatctggtc
     4381 ctggctttcc ctccctatac gcttcccatg tcccccccgt taggggtctg tagcttggcg
     4441 ggatatattc gacgaacgct cccagactgg aatgtccgcg tcttcgactt caacctcgat
     4501 tggcatcgct tgtttttcga ttccctcgga ccgagccgac gcccttcgtc gttgacgatc
     4561 gatgatacca atcttcgcta cgcggcagag ctttttcata cgggattggc cgaggatttc
     4621 catcagcgcg cgcggtattt gcactttgcc caggtctggg gcctggctat gcgtcaaccg
     4681 cgattcctgc gaaaaaacct cgaacccgcg tttctttccg gcaaaatgcc tgactatatt
     4741 cgacatcagg ccgatagggt tctcgaggaa aatccggaca tcgtagggat atccgtttgc
     4801 tacgccctgc aacgatggcc ggcgctctgt cttgcaaggg aaatcgccca acgctcctcc
     4861 attcccctcg tgatgggagg aacggttttt tccgacggcg tacccaaagg atggggcgcg
     4921 ggccatcgcg tagccgatta tgtcgtcatg ggagctggcg aacagcccct tgcagcgatt
     4981 ctcgaaggaa aagtcaacga tggccccatc ccgggcgtcg tacgcatcgt cgacgggaaa
     5041 gcctcttccg cggctcccat tcatgaatcg gacctcgatt cgctcggcac acccgatttt
     5101 tcggacttac ccctcgaccg atactatagc ccccagcccg ttttgcccat caccacatcg
     5161 agaggctgtt attggcgacg ttgcgctttc tgctcccatt ttcaatcgtc cgggcaggtc
     5221 taccagcttc ggtccatcga ggcggtcatc gacgagttgc gggagcatgt catgctgggg
     5281 gtacgccact tttacctcgc cgatagcgtg gtatctcctt ctcgttttcg acgattcgcc
     5341 caagccatcg tggattctgg cctgaaaatt cactattatg cgatggcgcg ggcggaaaaa
     5401 gctttcgacc gagagctgtt ggatctcatt tcccgttccg ggtgccggtt tattttatgg
     5461 ggattggaaa gcggcaacca gcgcgtgctc gacctgatgg ataagggaat ctcggccgaa
     5521 accacggaaa cgatcctgca aaccagtcgg gaggtgggcc ttcggaacca tgttttcgta
     5581 atttgcggct tccccacgga aacccgtcag gaattccaag atactattga ttttcttgtc
     5641 aagaataaag aaaacatcga tgttgtccac cgcgccatct tttcgatgga agaaaattcg
     5701 ctcgtacacc gctttccgga gaagttttcc atcaatgatt tttggccgaa agccactcct
     5761 cgacttttcg acttcaccac caccaaaggc atgacacgag cggaggtcaa agcggcattc
     5821 ctggcagctc gccccacgat gctttccttt tccggacacc cttccgttcc tggggatttc
     5881 aagttccgag agcattgcct gctgcattac gcgaaaaact ccgaagagcc gccaaagcat
     5941 gagtccccgt ccatgcgttt ggcgtagccc gcgatttgaa ttttcgagcg ccaataagcc
     6001 agtgcctacc acgcttcagg tcgacttcat ccccaagcga atcgccaaat ggtgaaggtt
     6061 gctgcgatgc atgccgagat ggcgcgcggt ggcggaccaa ttgccatgat gttcctgtag
     6121 ggtccgggcg atcaacgtgc gttgaaagtc ccgcgttgcc tggtgaagag tcatcgaagc
     6181 actggctttc gtaacaccag cctccagatc cgtttcggtc gtgacacggc tggcagcgaa
     6241 gtccgggccc agatgaaagc cgcggatgac caccggatcg gtgcgtgaca cgcccgaggc
     6301 ggcgttgagc gtgactctgg acaagacgtt ctcgagttcc cgaacattgc cgggccaggg
     6361 atagcgttga agcgccacca cggcttccgg ttccagacga acgggaccca aaccgaggcg
     6421 tcgccgcatc agatcgcaaa aatagcctgc caacagcggg atatcttcgg cccgctcccg
     6481 caaagcaggg accgaaaggg ggtagacgtt cagccgatga tacaaatccg cccgaaaccg
     6541 attggcctgc acttcccgtt cgagatctcg attggtcgcg gccaaaatcc gaacgtcggc
     6601 tttgatggct ttgtccgaac ctacccgctg gatttcccct tgttggatgg ctcgcaggag
     6661 tttgggctgc acggaaaggg gcaattcacc aatttcgtcg agaaataagg tccccccatt
     6721 cgccacttcg aacttgccca tccggtcgga tgatgcaccg gtaaacgatc ctttgacatg
     6781 cccaaacaac tcgctatccg ccaaggtttc gggcagggca gcgcaattga catagagcat
     6841 gggctcggcg tgacgcatcg aggcgttatg caccgcccgc gcgacgagtt cctttcccac
     6901 accggtctcg ccggtgatga gcacggtcaa gtcggaacgg gccaccaatt cgatgtcctg
     6961 ccgaagacgc tgcatcaccg ctgaagtgcc aatcatatcg ctgccttgcc gcaactccac
     7021 gtctcgcatc agatcccggg cgattcgtcc ctgccggtcg gcgcttcgct ccagcgcttc
     7081 aatgagttgt gtcgtacgaa gttccgcggc ggccagcgcc cccacggccg cgagaaaact
     7141 ccggtcgata tggtcaaaag catgaggctg ggcggcgtcg gcggtcagca cgcccagcaa
     7201 ctcgccgcgc acccgcaggg gacaacccaa acaggcatga atacgctgaa agtctccgag
     7261 atcgctggcc aaaagcccat cgtacggatc cgacaactcg ctatccactg gaaaaatcac
     7321 cggctccgtc gcctcgcaca ataggcgaag gcgcggctgt tcatccaacg caaagcgccg
     7381 tcccagggca tcgggcgaca atccttcggc cacgatggga accaaactcg tgtcttcgag
     7441 ccgcagcagc gccgcggcat cgtatggcac cacatttcta atggcggcca gcaagcggcg
     7501 ataacgctct tccgacgaca ggcctgcggt caggtcggaa gcgatggaga tgatggcatc
     7561 gagttggttc attgttgtca atcaaacaac atcgttatcg aaaacgcaac atccgtttgc
     7621 tgtgaaatcg acaacatcga tgagtatttt cgatgattct cagcaagtcg taccaggcac
     7681 atcccttgct gaacgtcgcc ccgtcatccc actgatgact ggaggaattc gatgaaaaat
     7741 accgaaagaa gccttggcga gctggtcacc accttcccca tggccgcgaa agtgttccaa
     7801 caataccaac tcgattattg ctgcggcgga aaacaatcgc tggcggatgc gtgcgcgaaa
     7861 gaaagcctgg accccgagac ggtgcttacg gccatcgagg cggcaagccg tgacaaaacc
     7921 gacgaaaccc gctgggacca gcgtcctttg cccgaattgg ttcagcatat tctcgaccgg
     7981 taccatgcgc cccttcgcac cgaattgccc aggctcattg ggctggccac caaagtcgag
     8041 gcagcccatg ccgaaaagcc cacttgcccc aagggactca ccgaattgtt gacgcgggtc
     8101 gccagggatg tcgaaaacca ccttgccaaa gaagaacaga ttctttttcc gctcatcgtc
     8161 gccggtcgcg gtcgaaccgc gcacatgccc gtgcaggtca tgatgcgaga acacgatgac
     8221 catggcgagg accttcgggc catccgtgaa atcaccaacc agcgaaaggt gccggaggat
     8281 gcttgtgcga catggcgcga gctttatcgt gcgcttgggg agttcgagac ggagttgatg
     8341 gatcatatcc atctcgaaaa caacatttta ttcccgaggg ctttggcgag ctagacgcca
     8401 aacgactccc catcgctcca cggaccattc gccgtctccc tgcccactcc gttcgtaact
     8461 aattgaataa acacgttttt ttatttcgcc ccctcaccat cccgggccta tgagggccat
     8521 ccaaacggtg ccgaccaggg tcgaaagcag ggtcagggga aagcccaccc gcaagtattg
     8581 ccagaagccg attccgccga tatcgcggct tgcttccgcg acgatgatat tggccacgga
     8641 accgagcagg gtcaggtttc cggcgaatgt cgacgccatg gctagcaatt cccagcccat
     8701 gatgggattg gccagcgagc ccatggcttc gcgaatgacc aggatgaacg gcacattgga
     8761 tacgatattg gagcccacaa ggaagatggc tgacagacga aaccattcca gcattccctc
     8821 ttgccggccg cccaacggat attgctgaaa aaaccacgcg ggcgcgccgc tgcggttcag
     8881 cccttccacc accacgaaaa gggaagcaaa aaacagcaat aaggaccaat cgatccgttt
     8941 ccacaggtgt tgggtatcgg tccgatggat gagcatgagc acggcaaagc cggccgtcgc
     9001 ggtccaggcc aggtcgcctc cgagggtata aaccacggta gtcgcggcga tgacccccag
     9061 cgtgacatgg ctatgccacg acccttccga acgcgtttcg gacttcgcaa aaggctcttt
     9121 gggcagtgtt tttcgaaata gccatcgcaa aatcccatgg ttgatcagca atccagcggc
     9181 cgccaccggt gccatgagca aaacatggtg tcgatacgtc aacccaccga gtagcccgca
     9241 aagcatattt tgtgggttcc ccacgagagt ggccacgctt ccggtgttcg aggcggtcgc
     9301 cagcgcaagc aaaaagggaa gaggcgtgag ccgatgcttt tggatgatgc gaaccaccaa
     9361 aggagcgccg agaatgcaga ccgcatcgtt ggtgatcagg gcactgaggg gaccggcggt
     9421 ccacacgata agtccgagca agcgtggggg cgtgcgggcc aacctcacca atccttcttc
     9481 tgcccgatcg aaaaagccat gcacggacaa aaatgctccc atccccatca caccgaaaag
     9541 caacagcaac gtcgcgccat cgaccgaatc aagagcttgg cgtggagtga ggatgccaaa
     9601 tacaacgaag gccacggccc ctacgagcgc tcctgcggga cgatcgaatc gtagccactg
     9661 caaacgacga gcggaaatga gtccgtaggt gatgataaac agggcaacac cagaccaacg
     9721 catggtcttc ggcctctact ccaaaaccat ggggagagtt cgacaattcg cggccatgcc
     9781 ttgcggtatt cgtggtagct cgaaaccagc aagcggacgt ttacttatcc acacattacc
     9841 gcataccaac accgtgtcat ctgtgccata cgggatgtgt cataaacctg gcgagtcact
     9901 ggcagccaat gaacagtcgc gttgtgtgtc ccaagcaata tgcgcgaaaa aggaatagtc
     9961 gtctttggca agcctattgc aatgcttccc cacgccatgc gttggttcca ccgaaaagca
    10021 tcgacggtca ggcatgataa ggacagcttg gagcagctag ccccccgttc cacgcttctt
    10081 cctcctgctc ctgccggtat gccaaatagc ccgcgaacca tgagggcccc catctgctcc
    10141 cccccccagg ccctaaggga gcttgatgtg gctgtcgaag aaatggccta cgacgatttc
    10201 gcctgggacc tcgaagaaga cgaacgtccc accaagcgct ggcaaccctt tcgtccggcg
    10261 gctcctaccc ttcgctgtcg attcgcctag tcgcgttcgg acaccgtttt acctccccta
    10321 ttccacgaaa aaacagttac tcacccgcgc atgccggaca gaaaactgct ctcgtctacc
    10381 gttcgggatg ctacgctacg cttgaatcat ccctgacaac ggagctggat atgcgaaaca
    10441 cgattgtgca gcggacggtg ttgagccgta tggcattggc tacgaccttg ctcatcgcgt
    10501 tatctcacgg tgcctgtggt tcggaggaga cgagcgggcc ctcgggcggc cctgtcatcg
    10561 gcacgggtgg aggcagcacg ggaggagttc gtaatccgac ttgtgccaaa ggaaccagcg
    10621 ccggcgaggt gcaagcccct gaattcgtga tgaatttacg gggtcagaca tcatggttcg
    10681 cctctcccat cgtctacgat ctcgacggcg atgggcgcaa cgaactcatt gccgcgtatt
    10741 attccgtcta tgttttcgac gagcagggaa aggagttgga ccgtgtgaag gctggcgacg
    10801 gacggattta tgcgccgcat gtcgtggccg atctcgacgg tgacggcatt acggaggtcg
    10861 tatttggcaa tgatcatcag gttttcgcga tggagtggcg cgatggaaag ctcgtcatga
    10921 agtccgggtg gccggtggat acgacgacgg cgggcaatgc tccggaggta cgggggctgg
    10981 cggcggggga tttggatggc gacggtttca ttgaggttgt ggccaccacc acacaaacgg
    11041 cgagcacgga aaaggggggg gcacaagtct ttgtatttcg ggcagatggc acgcttcacc
    11101 agccggcggg gatcgaatgg aaggcgtggc cgcgctacaa tgccgcagcg ggaaagggca
    11161 atgatgccga ccgcaacggc caaggccatc atggctacgg ctgtttcgga ttgaatgtgg
    11221 gaatcggcaa tatcgatgac gacgatgatt tggaaatcct tgtcacctat gacaatcatc
    11281 atatccaagc tttcgaccac gacggggttg cgcttgatgc ttccccttgg tttacgaatc
    11341 gtggttcgga ccatgcgggg gagcgtctga cgtggggtca gttcattcgt tgggcggacc
    11401 ctgaggtcga gaaaaatcac tatcacgacc atacagggga ctggcctcat ccgagttggg
    11461 cagagtggtt gcagtggacg gcgtcgccac cgaatgtggt ggatttggat ggcgacggga
    11521 aaaacgaggt gctgggagtg cccaacgtgg agatgcatga accgtacgaa acgcaagcgt
    11581 atgcggtgat ggtcttgcag ggggcgtatg gagacgggac tcgttcggcc atgcgaaaag
    11641 agggctggga cgtattgcca cgaggcaatc gtccgatttc ggtgaaaggc tactatccac
    11701 cgggagggat tccttcgccg accacggtga atcttcaagg cgacgatcga ccggagatcg
    11761 tggtatcgct caacgatggg ttcattcacg cgtttgacag cgaggcaaag gaaatatggc
    11821 ggtacaatta tacgtttggc aaagcggtga tggttgcgac ggaagtgatg gtggtggatt
    11881 tgaaccagga tggttcaccg gagtgtattt ttgcgacgta cggggcgccg gatgtgttgg
    11941 attcggggca tctggtgatt ttgggggcgg atggcaccga gcttcatacg gtggctttgc
    12001 cgaatccggg caaaaacggc aatggcaatg gggctcccgc tgcgccgacg gtaggggatc
    12061 tggatgggga tgggcagctc gagattttcg tgcagacctt tgaacatggg atggatgtgt
    12121 ttacggtgcc gggttcatcg acgcattgca tgctttggcc gacggcgcgg ggggggccgc
    12181 ttcggatggg gcaaccgaat ggtcattgag ccagggtggc gcggtgagcc cggcgatggt
    12241 tggctgcacc caatcggctg aaaacgttct agggtagatc gaggaatcgg ttgttcatgg
    12301 acaagctgaa gctcaagccg cttggggcct ttttgctggt gggcggattg gtgttgcttt
    12361 tgtacacgtt tcgaatggac ggtcccccct ggcaagcccg tcggagcgtg cagggagaag
    12421 tgctggagcg gtatacgtcg ccctcacgaa acgtcgcgga accggacctc attcttcgga
    12481 ttcgggtatg gcaaggagac aagccgcggg tattggaacg caaggtgcgg gaagaaacgt
    12541 ggaacgcgca tccgcagcgg tctcagatca tgctgacgct acagggagaa gagtggcaaa
    12601 aagccaacgt gcagggagac tctcgggaac attggatttc ttggataaag atagtaggcg
    12661 gtgctttggg agcgattttg ggggtggtgg tgctcgcgta tgaccgatgg tggaagtgat
    12721 gggatggttt gcggtgtttt cgggattccc tcggccgaat gaagtgctac caatgcagcc
    12781 cccgggttga tcggaagttg gccgggttgg agacgaccat gcgttcgaat cgagaagtgc
    12841 ctgctgagca agtgaccctt cggacagatc ccgagacact gacggacgag ttacaagcgg
    12901 tgggcgacag gttcgagttg ttgccggagc atacgcgcaa agcgttgacg ctgggtctat
    12961 ctcttcggaa ccctggcttt cacgtgtatg tggcgtgtga tccggatttg acgttcgaca
    13021 aggatattct gcgagcggcc gaggcggcgg cgcaggcgct acccgcgccc aacgatgtgg
    13081 ttttcgtcca tgacttcgag cgtccggaat ctcccaagcc cttgatattg cccgcggggc
    13141 gagggcgaga gcttgcgttg gaggtcgaca aggtcatcga cgagttggcg tatcggttgt
    13201 tgcatcttgg tgcgatgccg atcgtacgcg aagcggaaga agctttggcc aaagagctat
    13261 cgagcaagaa caagcaggtg gtgtcggacc tggagtcttt tgcgaaaacg cttggatttg
    13321 gggtcaaagc ggtgcccggg ggcgtacaga cgtttccgat tttgcatggc aagccggtga
    13381 gtcccgagca attcgaggtg ctcgacgaat cgaccaagcg tgctctggcg gatgcggagc
    13441 agaagttgtc cgcggcggtc gaagaatcgg ccaagcgtat tcatgagatg acggacaatg
    13501 tgaatgcggc aagcgatgat gcgcagcaga aagccgcgga gcaggtgatt ggcacgagca
    13561 tcgagccgct gttgcaatcg ttccgtggca tcgagccggt ggtggcgtat ttggagcgga
    13621 ttcgagagga attggcgcgc gattggcgag acttcaccga acctaccaac gaggccgatg
    13681 agcagcagga gtcgcgcgcc gacgaggtgg accggggcaa tcccgaagtg gcgaagcgca
    13741 cggggcgttt tcgggtcaac gtatttgtca gccatcgtcc cgacgcgaaa gcgccggtcg
    13801 tagatgccca tacaccaagt tttcccaatc ttttcggata cttagaacga cgcgcccgat
    13861 ttggcgcctt gctcacggac tttcttcgca tccgttcagg agcggtagcc catgcttccg
    13921 ggggaacgct gttggtgcgc gcggcggatc ttttggcgga tccgctgatt tgggagcgat
    13981 tcaaacgcgt gattcgagat cgggaattga cgatcgagga tccgatcggg ccggtcagtt
    14041 tatatgcaac cacgttgcga ccgcaaccga tacccttgaa tgttcgtgtg ctgctcattg
    14101 gttcgcagca tctttacgac cagctcatgc aactcgaccc ggacttcagc acgctatttc
    14161 gcgtcaaggt cgaggtgggc tggtgggtgg agcgaacgct ggacaatcag cgcaagctcg
    14221 actcgtattt gatgtcgtta ccgacggtga agcaaacgga agcgagtttt tcgcgcggtg
    14281 cccgtgcgcg tcttttggac tttgccacac gtctatccgg cgatagggaa cgtcttgccc
    14341 tgaccgtatt gcctctcgag gaaaccgcct gtttcgctgc cctgcgtgcg gccgagcggg
    14401 gagacggatt ggtcacggac gatgatatcg agaccgcgtg gcatgagcgt cgagagcgtt
    14461 cgggaagcat cgaacgggag gttcgggacc ttgccattcg gggcgagttt ttggtggata
    14521 cgtcgggaac gcgggtgggc gtgatcaatg ggttgagcgt attgacggcg ggggatgtgg
    14581 agtttgggca accgatgcgc atcacggcgg tggtatcgat tggacgtgat ggcgtgattg
    14641 atgtggagcg tgaggcgcag ctaggaggtt ctcttcacac caaaggggta gcgatcgttc
    14701 gtggtttttt gtcgttgctt tttggacagg agcgaccgct gagtttgcgg gtacagatag
    14761 cgttcgagca atcctatggt gaggttgatg gggatagcgc gagttcatcg gaactgtttg
    14821 cgattttgag cgctctggcc gatgtgggta tcgatcaagg catcgcggta actggctcgg
    14881 tgaatcagct tggggatatc caggccattg gtggcgtatg cgcaaaaatc gaaggatttt
    14941 ttgatgtatg caaggcgagg ggattgacgg gtgcgcaagg ggtgatgata cccaaaacca
    15001 atcttcggca tctggttttg cgaccggatg tggcaaaagc cattgcagac ggtcaattcc
    15061 atctatatgc gatctcgagt gtgagtgagg ggatcgaagt gttgacgggt ttgcaagcgg
    15121 gatcgcgtga tgccagcggg cgttttcccg ctggaagcgt gtttgggcga gtcgaacgtc
    15181 ggctgattga gcttgctgaa ttgcttcgtg atgcagaggc tggtcccgtg gatcggggag
    15241 ccattgcgga cacgagcgat ccggacaatg tcgatgcggg tgtggacttt cagaagcgtt
    15301 tcgatgggga ttggtgatcg ccccgtgaac gagcgagcgt gtggttgtca gccatgaaca
    15361 aatcaacttc attacctcct cctcgcttcc aggtcttcgt gggttttcat accgatgtag
    15421 gcaggcagcg tcagaaaaac gaggatgctt tggtcgaagt gggtgttccg ctcggctact
    15481 tgctcgcggt ggccgatggg atgggcggac acgattatgg tgatcgcgcg gcgatcatgg
    15541 cgctcaacac gatccaggaa agccttcggg aatccacgga tcaaccgcag gaaagcctcg
    15601 cgaagtcctt gaggaaagcc aatgccgttg tcttcaagga agcggaagaa gtggggcgta
    15661 cgatgggggc cacctgtgtc gccggtttga tctcgggtgg caagctttac gtggcccacg
    15721 tcggtgacgc ccgtgcctat ttgttgcgag cc
//
LOCUS       DAOSOR010000053        15496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_3828_length_15496_cov_4.097533, whole genome shotgun sequence.
ACCESSION   DAOSOR010000053 DAOSOR010000000
VERSION     DAOSOR010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 15496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15496
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3828_length_15496_cov_4.097533"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..838
                     /locus_tag="PK710_04605"
     CDS             269..838
                     /locus_tag="PK710_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09026.1"
                     /translation="MLARGTAIFSVTAMLSAVLLAGCCKNNNSSNNEEELPLIETPPP
                     TPIATDSVVQFSKVPLSVTLPAGWNQTQNTTQWLVYRPSNGGALVAMSGEKSCTLVNT
                     RFASALRELGLTNVVWGTPQNTTIHGLRAKIAAGTAIEANQLSYVKYSLTYAAGGQGC
                     LITLYNVWQSRADDFRATADQIILSVRPQ"
     gene            complement(1091..2650)
                     /locus_tag="PK710_04610"
     CDS             complement(1091..2650)
                     /locus_tag="PK710_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HOT09027.1"
                     /translation="MKKVSRYEPSDHPHESVKVFADYQGDIVDRCDVVVVGSGPGGAV
                     VAKELAEAGFDIVLLEEGLAWDAPDFVPDAGAAMVRMLREGGMRATRGNVFMPTMQAI
                     ALGGGSVVNSAICARSPAWVFDRWASLHGTELLTGASLDGHYQRVESFLGVEPTLEKV
                     LGERNLAFKRGCDALGMSSAPCPRNAPGCKGSAECFTGCRNRGKASMDVSYVPAAIRA
                     GARVYTSVRVDTVMASGRRARGVQGKAVDPRSKKAVYDVRVQARVVVLSAGCMATPVI
                     LQRSEIGNENGQAGRHLRFHPGTAVLAVFPDLIRPWEGATQGYHSLHELEDGLKYEVL
                     WSPPPIIATRFPGMGVQFKDQLARFERSAPFDAYVDGADSEGSVTGRPFGLEPDIRFT
                     FGAKDVKKLHRGLCRLVEISLAAGAVEVLPGLFGYKPVLRSQADLDELRAYRMKPTDP
                     VIAGTHAFGSTRMGNNVKTSVVDVDGRCHQTDNLYIADSGVIPSSPAVNPMLTIMALA
                     SRTAHILAARI"
     gene            complement(2647..3114)
                     /locus_tag="PK710_04615"
     CDS             complement(2647..3114)
                     /locus_tag="PK710_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09028.1"
                     /translation="MQNHPFSPLLSAALSKWKGALDRHEKQIVTACTEVLLPAGGAIA
                     VSGLDAKVVETFEQLLANVPPMTRMLLRAMLQFVERSPWAYGYSPRPFSRLDLSQRHK
                     VLERLMNSRFYLVRTAFLGLRTVLTVAYFRDENVMQALGATPNLDPFTQEKTP"
     gene            complement(3137..3859)
                     /locus_tag="PK710_04620"
     CDS             complement(3137..3859)
                     /locus_tag="PK710_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOT09029.1"
                     /translation="MAKPVGAVLRVAEVLLQRIVSEEYSPGLRLPSEVDLATEFSCAR
                     STIREALRYLASLNLVQSRQGSGAVVLDYRREGTLELMAPYFLHGRFDHPLPVLVSEM
                     LRTRTALACEAARLAARYARPESLSAIRKKIEKAHESRAHPLKHALEELNIFRAMTQA
                     SAVWPAVWFANAFMEPMREVHALVAEPLAAVQPDWLDTMNTLMDLIEARKAEEAVAHL
                     HRYFDRVDRQIEEGLTSLFSAS"
     gene            3939..4904
                     /locus_tag="PK710_04625"
     CDS             3939..4904
                     /locus_tag="PK710_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT09030.1"
                     /translation="MIEIDHHQTHNGDGWMLSLRRTVDPEKLDSERHPLLIIPGYGMN
                     SFIFGFHPRGTSMEAVLASHGFEVWSVDLRGQGRSRSVGGSLRYHLEDLVLYDVAAAV
                     RFVVGTTKTRAKDLDLIGCSLGATMMFAHVVCQKEHHGRRLVNMGGPVRWVQVHPLLR
                     VAFASPRVVEALPFRGTRHLARVALPALRRVPWLLSLYLHPSHIDMSRAGELSRTVEN
                     PNRHINADIARWMNLRDPMIGGRNIAEELKNIRLPLLTMVANADGIVPRATTLWPHET
                     IGSPQKKVVEVGTDDEPMAHADMFIHDRAPERVFNPLGDWLSTES"
     gene            complement(4945..5727)
                     /locus_tag="PK710_04630"
     CDS             complement(4945..5727)
                     /locus_tag="PK710_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09031.1"
                     /translation="MTHVTRELSACLSITALLVTACGGKVLDSASEHNDGGLAGAGGS
                     GATGGSGATGGSGATGGSGATGGSGATGGSGAYGGTETGGMGGYAASGATGGTGAYGG
                     APDGGTGAYGGTGTLPDGCPPLPPCNWCHGVVIYDTNGCAVGYRCENGVDPCVTPPCD
                     PSTPMSCGSHQHCEEDGLCWPGSECGKKNCTVTSIWGCNCAWSCPDGHDYATKCVPIG
                     SGDESCECLIDGKITFGCATSGGSAGGPGDWCGNPTCCGFPE"
     gene            5880..7388
                     /gene="dacB"
                     /locus_tag="PK710_04635"
     CDS             5880..7388
                     /gene="dacB"
                     /locus_tag="PK710_04635"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOT09032.1"
                     /translation="MRYRAAIPVFLSGLLFALGWMDLPIANSQTISTSSSSTAPSSPP
                     SSAAPQTHPRIQQAVDKLAQTIHEYGGTVGVYIVDIPTGKVLGAHEPKTALNPASNMK
                     LVTAGAALWKLTAHHTYRTGLYGQHKGQEVHQLVLRGYGDPSFEAKDLWELTQGLSRL
                     GVKRVRGDILVDQSFFDDAYVPPGFEQQPNEWAYFRAPVSAIALEANTVTMHVTATKS
                     GEPALVSFAPPGFVDMAGRVTTGAGGSEQSVTLTLTPQDGRLQARVGGSIPEDSIRMR
                     FTQRIDDPSLFAGYALRAILAERGIQVDGQVRAGGEKITRLLAMHHSSRLANLLERLG
                     KDSDNFYAEMIFKSLAGDTKRRGLTTASGAEVARAYLESIGAFDEGTLMRNGSGLFDS
                     NRLTAHTLGTVLRSSYQDPVVQSEFVRHLAVGGQDGTLRWRFRDKMTKGIVRAKTGTL
                     ASVTSLSGYVLPRNRNPIAFAIIVNGVAGKITGARDSIDRCVQTVVRESYKD"
     gene            complement(7576..8997)
                     /locus_tag="PK710_04640"
     CDS             complement(7576..8997)
                     /locus_tag="PK710_04640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT09033.1"
                     /translation="MDSKRPRIESDHPQDSVGMTGLAVAPLSSLADELEDTSVRVASR
                     FGRAEDMEVIISFSEPPPALETGARTSEKTGDSMLARYFREMATHHVMGPDDEIQAAE
                     KVETAEIAYWRAILSYVPAADYVLDGLEAAIKISGASEEVDLRAIPELRKLIRLFQKQ
                     RSKLTAEQERRYRRYCNELAAAVRLPDSDRLWVSHCCQVVHELVSPIENADEATNHTT
                     LADTPAYRRFLRRIRVAHERQLIAKSAFVKANLRLVVSIARRYNRGRMPLIDLIQEGN
                     IGLMKAVERFDHRRGYRFSTYASWWIRHAISRALADKGRAVRIPVHMLDTHNRVARAT
                     QAVIARTGREPTLEELESETGIPQDKLDKVKDFFAETPFSLDRPVGDEDGRKFIDFLQ
                     EDDAATPYDHLANRKWADEVRRLLTTLSPIESRIIRWRFGLDDENELTLKEIGDKYNL
                     SRERIRQLQEQALTKIRKQMRDF"
     gene            9354..10976
                     /locus_tag="PK710_04645"
     CDS             9354..10976
                     /locus_tag="PK710_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT09034.1"
                     /translation="MSRIGHQSFSRLRVSSICGWVAVHHPGWALLLVIAGVLHAVFHG
                     AVAWMAASLGRAIAAGSTAPMAGSAIVVASLGLGAVFAKGLFGILASLAQTRLSSAAG
                     QALRDHILSILLATGSHAPGPATITRLSSRLREAEAAIANGPFAVARAILQLLPIIAG
                     LFVVSPGLTVLAIAMLAPFSLVLSLARRRWRHAYESSMRASDAVHEEMDDLIRHLDLW
                     RCYGSGGQVREVVSELGARAASAHLRAQTLGVTISSTNEVLGATALLAVLVVAAHGFG
                     TIDGASVIAFIALFFLAYRPLRDLGDGRTAWLRGREALRTLSQLPVSTETSAVAGTIP
                     RAEPSVHVRIERVGVPPMTPCVSFDLPAGRMVAIAGPTGAGKTTLVRALLGLEPSAVG
                     EVSVNGYALAPGQVGPAYRPFAWVPQDAPVISGSLDDNLRLGGATCSHAYGHLAALGA
                     GALMQDIGEDKLGACGRTLSGGERRWVSLARALATGFPVLLLDEPTVGLDARARKAAL
                     EVLQRIKGKRSLVVASHDQDVLSMSDEVVTLE"
     gene            complement(11041..11997)
                     /locus_tag="PK710_04650"
     CDS             complement(11041..11997)
                     /locus_tag="PK710_04650"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT09035.1"
                     /translation="MRDDSHAISPGASALSVGIDSFYRSLSWIGSRWPSADPARYGIH
                     VVQDVAYGLDSPMHRLDVWVPSKFPAPRPSLLYLHGGGFRILSKETHWIMALSFARRG
                     YVVFNANYRLAPQHPFPAPLQDACLAYQWVVQNAHRYGADVQRLVLAGESAGANLATA
                     LTVAACFERKEPYARNVFDTTIVPKALLPACGILQVSNPERFLDDPAIHPWVMNRIVS
                     VSKAYLPDPKVEGADLASPLLVLESTACPVRPLPPCHAIVGADDPIRRDSLRLEKALS
                     HRASPVEVRSYPRQGHAFHAFVWRAQAKAAWKDTFHFLDQNL"
     gene            12195..13583
                     /locus_tag="PK710_04655"
     CDS             12195..13583
                     /locus_tag="PK710_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT09036.1"
                     /translation="MPQQIRQQEMVRVDGLTKGQRWQDISLLLTGPWVPASVAGMRLT
                     LIALLLFVLHTLACSSLSASRPAKEGTAEELRSALREDFRYGRLDRDAVRDLAYLVGE
                     REVRESKGEQAIQRVQQARGCARDLEDVLEDRAEGTDKAAALAAMALLAIDRGRPSAW
                     RSHRTSSDPDWRAVGVRTLTLAEEGQWRRAAMVDLDQNVRLAAAQASEKAMDPADRAA
                     LLDVARNDPEVLVRVAAIRALGWIATEQDVHFMRDLWPLAPAPARQALVSAWAFPGTM
                     ERRGLEQIRWVAETETGTPAIIAAGILYRYGESTRGVGAAMLRKAIQQGIARDRVFAI
                     AMAPYREPQWLELLKETAEQAEPIVRVAALEKLALHPETAAMAREKLGHIAVSGQAGQ
                     HEARQSMARVGDRRVLRLLLQDAKSSKPSVRLSAMHSLLTLDEWARASFFLADPEPSA
                     RMHTACALLSKP"
     gene            13680..14579
                     /locus_tag="PK710_04660"
     CDS             13680..14579
                     /locus_tag="PK710_04660"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HOT09037.1"
                     /translation="MAKVLIVGLGMLGMTGCIQQMLVDEQLASTSKAMPGINTVGDFE
                     VARTSVYASLGTLEGLHRLSPDNAEGLFLLTKGWAGAGTSFIEDEWETAEDAGNETMA
                     EYHRTRARAAYTRAVHYGVERLEQKADGFERARRNPEVMKRWLAAFEKEDVPSLFWTG
                     YAWIARVGVSKDIPQLVRELGIGVAMIERVVELDETYEHGLAHVILGTFHARMPTAEL
                     DKAQQHFERAFQINQGRLLITKVQYARTLYCWKKDKSNYEKLLQEVIDAGDPLPTERL
                     SNMISQRRAQRLLSDARMSRCGF"
     gene            complement(14857..>15496)
                     /locus_tag="PK710_04665"
     CDS             complement(14857..>15496)
                     /locus_tag="PK710_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOT09038.1"
                     /translation="VSYAEIDAPHGHDAFLLDDPREAVVWRGPMLHGALMQFLQDVHW
                     GDLDYLFLDLPPGTGDIPLTLSQKLQVTGAVIVTTPQDVALFDVYKSVSMCQKVNIPI
                     LGIVENESYFICDGCEKRHEIFGKGGGTKVAEMANAPLLAQIPMDTACRKAGDAGTPV
                     VMASPESPSALALRDAAEALAFRVAAFHAERSSTVISIDRSGGKNKHLPVVS"
BASE COUNT         3233 a         4265 c         4708 g         3290 t
ORIGIN      
        1 ggaagaccat gaacttgtca agatcaaaat cctcgaggaa tcgcctcaag accaggagca
       61 agcggcagtc atgattgccc aggcaacgca ggccaccgag gtgcaagtcc tgggtcgaac
      121 cgtgcttttg taccgacctc atcccgaaaa acccaaaatc acattgccct gaaaaaacga
      181 cttcgtgacc cattctccca attgactatc gaatacccgg cggttttggg tatacttgcg
      241 ctctattcat ccgggaggag ccattgccat gctcgcgaga gggaccgcga ttttcagcgt
      301 gaccgctatg ctcagcgcag ttttgctagc gggctgctgc aaaaacaaca actcctcgaa
      361 caacgaggaa gaactgccgt tgatcgagac gccgcctccc acgcccatcg ctaccgactc
      421 ggtggtgcaa ttcagcaaag ttccgctgtc ggtgacgctt cccgccggat ggaaccaaac
      481 ccaaaatacc acacaatggt tggtatatcg gccttcgaac ggaggggctt tggtcgccat
      541 gtccggagaa aaaagctgta cgctggtgaa cacccgcttc gcaagcgctc ttcgggaact
      601 cgggctcacc aatgtcgtat ggggaacacc tcaaaacacg accatccatg ggctccgagc
      661 caaaatcgcc gcgggcaccg ccatcgaagc caaccaattg tcctacgtca aatattccct
      721 cacgtacgcc gcgggcggcc aaggctgcct catcacgctg tacaacgtgt ggcaaagccg
      781 cgccgatgac tttcgcgcca ccgccgacca gatcatcctt tcggtccgcc ctcaatagac
      841 tgccacctac gcttgccgcc tcgtcgtacc cctccctgct cgttgcgaac gtcagcctcc
      901 cggcttgggg ccaatccaaa aaaactttat tattccaata gatacaccga tcgcggcagc
      961 ttcatccccc gtgttgagta aatatcgtcc gcgccgacac gccgcgccat gaactccttg
     1021 gctcggttca acaagcgtgt tttccgtggt tctcggaggc tagcagcgcc gaacgcccag
     1081 gaaaaagaga ttaaatccgc gccgccagga tatgggcggt tcgcgacgcc agcgccataa
     1141 tcgttagcat gggattgacc gcagggctgg agggaatcac accggagtcc gcaatatata
     1201 ggttatccgt ctgatgacat cgcccatcga catccaccac gctcgttttg acgttatttc
     1261 ccattcgcgt ggagccgaag gcgtgggttc ccgcaatcac ggggtcggtg ggtttcatgc
     1321 gataagcgcg caattcatcc aggtcggctt gggaacggag cacgggttta tagccgaaca
     1381 ggccgggaag cacctccaca gctccggcag ccagcgaaat ttccacgagc cggcataatc
     1441 cccggtggag ctttttcaca tctttggccc cgaaggtaaa acgaatatcg ggttccaagc
     1501 cgaagggccg cccggtcacc gaaccctccg aatcagcccc atcgacatag gcatcgaaag
     1561 gcgctgaccg ctcgaaacgc gccaattgat ccttgaactg cacacccatg cctggaaaac
     1621 gcgtggcaat gatgggcggc ggggaccata acacctcgta tttcaagcca tcttccagtt
     1681 catgcaagga atgatatccc tgggtcgccc cctcccaggg gcgaatcaaa tccggaaata
     1741 cggctaaaac ggcggttccc gggtgaaaac gaaggtgacg tcccgcctgg ccgttttcgt
     1801 tgccaatctc cgaacgctgc aaaatcaccg gcgttgccat gcatccggcc gacaacacga
     1861 ccacacgcgc ctgtacccga acatcgtaca ccgctttttt cgaccgggga tcgactgctt
     1921 ttccctggac accacgggct cgacgtcccg aagccatcac cgtgtccaca cgcacactcg
     1981 tatacaccct ggcccccgct cggatcgccg ccggtacata ggagacgtcc atcgaggcct
     2041 ttcctcgatt gcggcagccc gtaaaacact ctgccgaccc tttgcatccc ggagcattgc
     2101 gcggacacgg ggcactcgac atgccaagcg catcacagcc gcgtttgaac gctaaattgc
     2161 gttctcccag cactttttct agcgtcggtt ccaccccaag aaacgactcc acccgctgat
     2221 aatgcccatc gagcgaagcg cccgtgagca attccgttcc atgcaggctt gcccaacgat
     2281 cgaaaaccca cgcaggcgag cgggcgcaga tggcagaatt gacaacggaa cctcccccca
     2341 gggcgatggc ctgcatcgtg ggcatgaaga cattgcctcg ggtggcgcgc atcccgcctt
     2401 cccgcagcat gcgcaccatc gccgcgccag catccggtac gaaatcgggc gcatcccatg
     2461 caagtccctc ttccaagagc acgatatcga aacccgcttc cgccaattcc ttggctacca
     2521 ccgctccgcc gggtccggaa cccaccacca ccacatcgca acggtcgacg atatcgccct
     2581 gatagtccgc gaaaactttg accgactcgt gagggtggtc ggacggttca tagcgagaca
     2641 cctttttcat ggcgtttttt cctgggtgaa agggtccaag ttcggtgtgg ctccaagcgc
     2701 ttgcatcacg ttctcatctc gaaaataagc gacggtaagt accgtccgaa gcccaagaaa
     2761 cgccgtgcgc acaagataaa accggctgtt catcaaccgt tccaacacct tatgccgctg
     2821 ggacaagtcc agacggctga acgggcgcgg cgaatatcca tatgcccacg ggctgcgctc
     2881 cacgaattgc aacatcgcgc gcaacaacat gcgggtcatg ggcggcacgt tcgccagcaa
     2941 ctgttcgaat gtctccacga ctttcgcatc cagtccggac accgcaatcg cgccccctgc
     3001 tggaagcaaa acctccgtac aagcagtcac gatttgtttc tcgtgacgat cgagcgcccc
     3061 tttccatttc gagagggcgg ccgacaacaa gggggaaaaa gggtggtttt gcatggtcat
     3121 cctcgcgggc aaagggtcag gacgcggaaa agaggctcgt caaaccttct tcgatctgac
     3181 ggtccacgcg gtcgaagtag cgatgcaagt gggccacggc ctcttcggct ttccgcgcct
     3241 cgatgaggtc catcaacgtg ttcatggtat cgagccaatc gggttgcacc gccgccaacg
     3301 gctcggccac caacgcatgc acctcgcgca tgggctccat gaatgcattg gcgaaccaaa
     3361 ccgcgggcca tacggcggat gcttgcgtca tcgcgcgaaa gatattcagt tcctcgagcg
     3421 catgtttcaa aggatgggca cggctttcat gggccttttc gatttttttc cgaatcgccg
     3481 aaagggattc cggacgtgca tacctcgcgg ccaatcgcgc ggcttcacaa gcaagggcgg
     3541 ttcgcgtccg aagcatctca ctcaccagca ctggtaacgg atgatcgaat cgaccgtgca
     3601 aaaaatacgg agccatcagc tccagcgttc cctcccgacg ataatccaat acgacagcgc
     3661 ccgagccttg acggctttgc acgaggttga ggctggcgag atagcgaaga gcttcccgaa
     3721 tggtgcttcg cgcgcaggag aattcggtgg cgagatcgac ttccgaaggc aaacgcaatc
     3781 ctggagaata ttcctccgaa acgattcgtt gcagcaggac ttcggccacg cgcaaaacag
     3841 ccccaacagg tttggccatg accttcatag tagtctgaca acttagtctg tcaactttaa
     3901 ccgtgacctc ggaccgaatt catgctctga ctactttcat gatagagatc gaccatcacc
     3961 agacccacaa cggcgacggt tggatgctgt cgcttcgtcg cacggtagac ccggaaaagc
     4021 tcgattccga gcggcatccg ctgctcatca ttccggggta tgggatgaat tcgttcattt
     4081 ttggttttca tccacggggc acatcgatgg aagcggtgct ggcctcgcac ggtttcgagg
     4141 tttggtccgt ggatcttcgt gggcagggaa ggagccgctc ggtaggggga agccttcgct
     4201 accacctcga ggatttggtg ctttacgatg tcgcggcggc ggtgcgcttc gtggttggaa
     4261 cgacgaagac ccgcgccaag gatctggatt tgatcggctg cagtctgggg gcaacgatga
     4321 tgttcgccca tgtggtatgc caaaaggagc atcatgggag gcggttggtc aatatgggtg
     4381 gtccggtgcg ctgggttcag gtccatcctt tgctgcgggt ggccttcgca tcaccgcggg
     4441 tggtggaagc tcttcctttt cgcggcaccc ggcacctggc gcgcgtcgcc ctacccgcgc
     4501 ttcgtcgtgt tccctggctt ctttccttgt atcttcaccc gagccatatc gacatgtccc
     4561 gtgccggcga actgagtcgt accgtcgaga acccaaaccg ccatatcaac gcggacatcg
     4621 cgcgatggat gaatttgcgc gatccgatga tcgggggtcg caacattgct gaggaactca
     4681 aaaatattcg gctgccgctt ttgacgatgg tagccaacgc ggatgggatc gtacctcgag
     4741 cgaccacgtt atggccgcat gaaacgattg gttccccaca aaaaaaggtg gttgaagtgg
     4801 gaaccgacga tgaaccgatg gcccatgcgg atatgttcat ccatgatcga gcgccggagc
     4861 gggtgttcaa ccctctcggc gattggctgt cgacggagtc atgaggattc ggttttgttg
     4921 cctgtcagcg aagcattgcg gcaactactc gggaaaacca cagcacgtgg ggtttccaca
     4981 ccaatcaccg gggccgcctg ctgacccccc actcgtcgcg cagccaaacg tgatcttgcc
     5041 atcgatgaga cactcgcacg actcgtcacc agagccgatg ggaacacatt tggtggcgta
     5101 gtcatggccg tcgggacatg accaagcgca attgcatccc caaatggagg tcaccgtaca
     5161 gttttttttc ccgcactcgg accccggcca gcagagccca tcctcctcgc aatgctgatg
     5221 acttccgcaa ctcatggggg tgctcggatc acaagggggc gtcacacaag ggtctacgcc
     5281 gttttcgcac ctatatccca cagcgcagcc attggtgtcg taaatgacca cgccatggca
     5341 ccaattgcag ggtggcagcg gcgggcaacc atccggcaaa gtaccggtgc caccgtaagc
     5401 tcccgtgcct ccgtccgggg cccccccata cgctcctgtt ccgcctgtcg cccctgaagc
     5461 cgcgtatccc cccattcctc cggtttctgt acccccatac gctcccgaac ctcctgtcgc
     5521 gcctgaacct cctgtcgctc ccgagcctcc tgtcgctccc gagcctcctg tcgctcccga
     5581 gcctcctgtc gcgcctgaac ctcccgcgcc cgctagccca ccgtcgttat gctctgaagc
     5641 agaatccaat actttcccac cacaagcggt aacgagaagg gcggtaatgc tcaagcatgc
     5701 ggaaagctcc ctagtcacgt gagtcatggc cttgcccctt agattcgaca ttcgaaagac
     5761 gaagtatagc agacgagaag ggggctgcca ttttcgaggc ggctaaaaac ggcttcgtca
     5821 tcggcgatcg gcacctgtga gctagatcat ccctatccaa agatgctagg gcatcaggta
     5881 tgcgttatcg agcggcaatt cccgtgttcc tgagtggctt actcttcgcc ctcggatgga
     5941 tggacctccc catcgcgaat tcacaaacaa tttcaactag ttccagttca acggcgccat
     6001 cgtcgccccc ctcttccgcc gccccgcaga cccatccacg catccaacaa gccgtcgata
     6061 aactcgccca gaccatccat gaatacggcg gaaccgtcgg cgtgtatatc gtcgatatcc
     6121 ccaccggcaa agtccttggt gcccatgagc cgaaaacagc gctcaatccg gcgtcgaaca
     6181 tgaaattggt gacggctggg gcggcgcttt ggaagctcac ggcccaccat acgtatcgca
     6241 cgggactgta cgggcaacac aagggccaag aagtccacca gttggtattg cgcggctacg
     6301 gtgatccttc ttttgaagcc aaggatttat gggaactcac gcaggggctc agccggttgg
     6361 gcgtcaagcg ggttcgaggc gatatcttgg ttgaccagtc attttttgac gatgcgtacg
     6421 tgccgccggg atttgagcaa caaccgaatg agtgggcgta ttttcgagcg ccggtgagcg
     6481 ccatcgcgtt ggaagcgaat acggtgacga tgcatgtgac ggcgacgaag agcggcgagc
     6541 cggctttggt ttcgtttgct cccccggggt tcgtggacat ggcgggtcgg gtgacgacgg
     6601 gggcaggagg gtcggaacaa agcgtgacgc tgaccctgac gccgcaggat gggagattac
     6661 aagctcgtgt gggaggctcg atccctgagg attcgatccg gatgcgcttc acccagcgta
     6721 tcgacgatcc tagtttattc gcgggatatg ctcttcgagc catccttgcg gagcgaggca
     6781 ttcaggtcga tgggcaagtt cgggcgggag gtgaaaagat aacacgactg cttgcgatgc
     6841 atcattcctc ccggctcgcg aatctgctcg aacggttggg aaaagacagt gataattttt
     6901 atgcggagat gattttcaaa agtttggcgg gagatacgaa acgtcgcggt ctaacgacgg
     6961 cctccggcgc cgaggtggcg cgcgcctatc tcgaaagcat cggagccttc gatgaaggaa
     7021 cgctcatgcg caacggttcg gggcttttcg acagcaatcg tctgacggcc cacaccctgg
     7081 gcaccgtttt gcggtcgtcg tatcaagatc ccgtcgttca atctgagttt gtgcgtcatt
     7141 tggcggtggg ggggcaagac gggacgcttc gatggcggtt tcgggacaag atgaccaaag
     7201 gcattgtgag ggccaaaacg ggtaccttgg catcggtgac atcgctatcc ggatatgtat
     7261 tgccgaggaa ccgaaatccg atcgcgttcg ccatcatcgt caacggggtt gcgggaaaga
     7321 taacgggggc gcgggattcc atcgatcgat gcgttcaaac cgtggttcga gaaagctaca
     7381 aggattgaaa atcggttgtg ttgaaaatcg acctggcatc cgttcggaaa cggggtaaac
     7441 tccaggttag ggaaatcgtg actccgcaag gaggagaggc accatgatcg caatgtcatg
     7501 gtgagggggc atcgtgtggt catgtatcga gctgtaaacc aacgcggtac atgagcccgg
     7561 caggggggat atttctcaga aatcacgcat ttgtttgcgg atttttgtca gggcctgctc
     7621 ctgcaattgg cgaattcgct cgcgagacag attgtatttg tccccaattt ccttgagagt
     7681 cagctcgttt tcgtcgtcca aaccaaaacg ccatcggatg atgcgcgatt cgatgggcga
     7741 caacgtggtg agaagccgtc gcacttcgtc ggcccatttg cgatttgcca ggtggtcata
     7801 gggtgtggcg gcatcatctt cctgtagaaa atcgataaat tttcgtccat cttcatctcc
     7861 gacgggtcgg tcgagggaaa atggggtttc ggcaaaaaag tccttgacct tatcgagttt
     7921 gtcttgaggg atgccggttt cggactcgag ttcctccaag gtaggttcgc ggcccgttcg
     7981 cgcgatgacg gcctgtgtgg ctctggcgac tcgattgtgg gtatcgagca tatggacggg
     8041 gattcggacg gcgcgtcctt tgtcggccaa ggcacggctg atggcatgcc ggatccacca
     8101 ggaggcatag gtcgagaacc ggtatcctcg gcgatgatcg aagcgttcga cggctttcat
     8161 caagccgata ttgccttctt gaatgaggtc gatgagaggc atcctgccgc ggttgtaacg
     8221 tcgagcgatc gacaccacca atcgcaaatt cgccttaaca aacgcgcttt tagcaatcaa
     8281 ttgccgttca tgggccaccc ggatacgtcg caggaagcgg cgataagcgg gcgtatcggc
     8341 gagtgtggta tggttggtgg cttcgtcggc gttttcgatg gggctaacga gttcatgaac
     8401 gacttggcag caatggctta cccacagacg gtcactgtcg ggcaggcgga cggccgcggc
     8461 gagttcgttg cagtatcgtc ggtaacgtcg ctcctgttcc gcggtcaatt tggagcgttg
     8521 tttttgaaaa agtcgaatta gtttgcgcag ttcggggatg gctcgcaggt cgacttcttc
     8581 ggaggcgcct gatattttga tggcagcctc gaggccatcg agaacgtaat ccgcggcggg
     8641 gacatacgat aagatggcac gccagtatgc aatctctgcg gtttcgactt tttccgcggc
     8701 ttggatctca tcgtcggggc ccatgacatg gtgggtagcc atttcccgga agtaccgggc
     8761 gagcatcgaa tccccagttt tttctgatgt tcgtgcgccg gtttcgaggg cggggggagg
     8821 ttcggagaaa gagatgatga cctccatatc ctctgcccta ccgaagcggc tggccacgcg
     8881 aaccgacgta tcctccaact catctgccaa cgaggacagg ggtgctacgg ccagccctgt
     8941 catccccacg ctgtcctgcg gatggtcgga ctcaatcctc ggtcgcttag aatccatggt
     9001 ccaattccga gctgtcgaag acctcatctt ttcacctaac ctatcatccc ctcgggttat
     9061 tcccacgtgt tgtggttgac ttctcgcgtt tgctctcctc atcggctgtg ttcgcctctc
     9121 atttctgctc acagctcgtt tcatctctga accatcgtgc accagatcct tggcgcgatt
     9181 ggtgccacgt attctgggtt tgaattgtca agcaataatc gttccgcgac ggagcatcga
     9241 agaatcattt cgagccaagg ctcgttgaaa cggaaatatg agttctgcct ccggggctcg
     9301 gcatatttgt gtcacagtgc caaaagctcg cctgcggcta acccctgatc ctcatgtctc
     9361 gcattgggca tcaatccttc tcccgtcttc gcgtttcgag catttgcggc tgggttgcgg
     9421 ttcaccatcc gggctgggcc ctgctgctcg tgatcgcggg ggtgctacat gcggtatttc
     9481 atggagcggt ggcgtggatg gctgcttcgt tgggtcgtgc gatcgccgca ggttcgacag
     9541 caccgatggc ggggtcggcc attgtcgtag catccctcgg attgggtgcg gttttcgcga
     9601 aagggttgtt cggtatcctg gcgtcgctgg cacaaactcg tttgtcttcg gcagccggtc
     9661 aagcgcttcg agaccacatc ctttccattc tgctcgcgac aggctcgcac gcgcctggtc
     9721 ccgcgaccat cacacggttg agttcccgtt tacgcgaagc cgaagcagcg atagccaacg
     9781 gaccgtttgc tgtcgcgcgc gccatccttc aacttcttcc cattatcgcg ggtcttttcg
     9841 ttgtttcgcc aggattgacg gtgcttgcca tcgccatgct cgcgccgttt tcactcgttt
     9901 tgtccctggc acgacgtcgt tggcggcatg cttatgaatc tagcatgcgc gcatcggatg
     9961 cggtgcatga ggagatggac gaccttatcc gacatctcga tctatggcgt tgctatggat
    10021 cgggtgggca ggttcgtgaa gtggtttccg agcttggggc ccgtgcggcc tcggcacatc
    10081 tccgagcaca aacgctaggc gtgaccatat cctcgaccaa cgaagtgctt ggagccactg
    10141 ctttgctcgc ggttttggtc gttgccgccc atgggttcgg caccatcgat ggagcatccg
    10201 tgatcgcctt catagccctt ttctttttgg cttatcgacc gttacgggac ttgggggatg
    10261 ggagaaccgc ctggttgcgt ggacgtgaag cccttcggac cttgagccaa ctgccagtgt
    10321 cgacggaaac gagtgctgtc gccggtacta tcccgcgagc ggaaccatcc gttcacgtgc
    10381 gcatcgagcg agttggtgtg ccaccgatga cgccttgcgt aagctttgac ctgccggctg
    10441 gtcgtatggt tgcgattgcg gggccgaccg gcgctggcaa gacgactctc gttcgggcgt
    10501 tgctcgggct ggagccatcg gcggtgggag aagtgtccgt aaacggatac gctttggctc
    10561 ccgggcaagt gggcccagcc tatcgtccgt ttgcctgggt tcctcaagat gcgcccgtga
    10621 tttccggttc tctcgacgac aacttgcgtc ttgggggggc gacttgttcg catgcttacg
    10681 ggcatttggc ggcattgggg gctggggcat tgatgcagga cataggcgaa gacaagcttg
    10741 gagcctgtgg gcgcactttg tcgggtgggg aacgtcgatg ggtgagtctt gcgcgggcgc
    10801 ttgcgacagg gtttccggta ttgttgttgg atgagccgac ggtaggtctc gatgcacgtg
    10861 ctcgaaaggc ggcgttggag gtgcttcagc gaatcaaggg caaacgaagc ctggtggtgg
    10921 cgagtcacga tcaagacgtg ctgtcgatgt ccgatgaagt cgttacgttg gagtaagatt
    10981 tggatttcgc agccaagcgt gcgctcatcc aaggggtggg aacgctcaac cgaatcgaca
    11041 tcacaggttt tggtctaaaa agtgaaatgt atctttccat gccgccttgg cttgggcgcg
    11101 ccagacgaag gcgtgaaacg cgtgtccttg tcgtgggtac gagcgaactt ccacgggcga
    11161 agcgcgatgg ctcagggctt tttcgaggcg aagagagtcg cggcggatgg gatcgtcggc
    11221 ccccacgata gcgtggcaag ggggcagcgg gcggacggga caagccgtgc tttccagcac
    11281 gagcaggggg cttgccaagt ccgcaccctc gacctttggg tcgggcaggt aggctttgga
    11341 tacggaaacg attcggttca tgacccaggg atgaatggcc ggatcgtcta aaaacctttc
    11401 tggattcgat acttgcagaa ttccgcacgc gggcaggagg gctttgggaa cgatggtggt
    11461 gtcaaacaca tttcgtgcgt acggttcttt tcgttcgaaa caagcggcga ccgtaagagc
    11521 ggtggcgaga ttggctccgg ccgattctcc cgcaaggacc aatcgttgca cgtcggcgcc
    11581 atagcgatga gcgttttgga cgacccattg ataggccagg caagcatctt ggagcggggc
    11641 ggggaatggg tgttggggag cgagacggta gttggcgttg aagacgacat acccacgcct
    11701 ggcgaaggaa agggccatga tccaatgggt ttccttcgac aggatgcgaa agccgccgcc
    11761 atgcagatac agcaaagatg gtcttggggc cgggaacttg gagggcaccc atacatccag
    11821 acgatgcatg gggctatcca agccgtaggc tacatcttgt acgacatgaa taccgtaccg
    11881 cgctggatcc gcggacggcc agcggctacc gatccaagac aacgagcggt aaaagctgtc
    11941 gataccgacc gaaagggcgg atgcgccagg ggagatggca tgagaatcat cgcgcatgag
    12001 catcgaccgt gaacgagaca gaagcgaatg gcaagagttc gtcgcgggga atcactcgtg
    12061 gagccaatcc accgcgcctg ggcaagacgc agaaagtcga ttggcaccat gaccacaatc
    12121 gctaaagaag tcggccggtt cgatgaaatc accgtcgtcg tcgtgagcga gcatgttgat
    12181 gaggctctga acgcatgcca cagcagattc ggcaacagga gatggttcga gtcgatggtc
    12241 tgacgaaggg gcaacgatgg caggacatca gcttgctgct gacaggcccg tgggttccag
    12301 ctagcgttgc gggaatgcga ttgacactca tcgccctttt gttgttcgtg ttgcacacct
    12361 tggcctgctc tagcctatcg gcatcgaggc cagcgaaaga gggaacggcc gaggagctgc
    12421 gtagcgcctt gcgcgaggat tttcgctacg gtcgtctgga tagggatgcg gtacgcgacc
    12481 tggcatacct ggtaggtgag cgagaggtgc gggaatcgaa gggggagcag gccattcagc
    12541 gggtgcagca agcccgcggg tgtgcgcggg acttggagga tgtcttggag gatcgggcgg
    12601 aaggcaccga caaggctgcc gcgcttgcgg ccatggcgtt gttggcgatc gaccggggac
    12661 gtccgagtgc gtggcgttcg catcgaactt cgtcggatcc ggattggcga gcggtgggag
    12721 tgcgaacgct gacgttggcc gaggaggggc aatggcgtag ggcggcgatg gtcgatctgg
    12781 atcagaacgt tcggttagcg gctgcccaag cctctgaaaa agcgatggat ccggcggatc
    12841 gggcggcgct tttggacgta gcgcgcaacg atccggaggt actggttcgg gtggcagcga
    12901 ttcgcgccct tgggtggata gcgaccgagc aggacgttca tttcatgcga gatttatggc
    12961 cgttggcgcc agcaccggcg cgtcaggcgt tggtgagcgc ctgggcattt cctgggacga
    13021 tggagcgcag gggattggag caaatccgtt gggtagcgga gacggaaacg ggaacgccag
    13081 cgatcattgc cgcggggatt ttgtatcggt atggtgaaag cacacgggga gtcggggcag
    13141 cgatgcttcg caaagcgatt caacaaggaa tcgcgagaga tcgcgtgttt gcgatagcca
    13201 tggcgcctta tcgggagcca cagtggctcg agctattgaa agaaactgcg gagcaagcgg
    13261 agcccatcgt gcgtgtggct gcattggaga aactggcgtt gcacccggag acggccgcga
    13321 tggcgcggga aaaactcggt catattgcgg tatcgggaca agcgggacaa cacgaggcaa
    13381 ggcagagtat ggcgcgcgtg ggtgatcgtc gggtactgcg gctgcttctt caagacgcga
    13441 aaagttccaa gccatcggtg cggctatccg cgatgcattc gttgcttacg ctcgatgaat
    13501 gggctcgtgc gtcatttttc ttggcggatc cggagccttc ggctcgcatg cacaccgctt
    13561 gtgctctttt atccaagccg tgaaaggcaa gacgacgcgt gtattcgatg acgggatatg
    13621 caaaggtaac cgcacgtttc aaggacgcaa ggggttcgag gatgggtatg cataggaaga
    13681 tggcgaaggt gcttatcgtt gggctgggga tgctggggat gacgggatgc atccagcaga
    13741 tgttggtgga tgagcagctt gcatccacca gcaaagccat gcctggaatc aacacggtgg
    13801 gagatttcga ggtggcacgc acttcggtgt atgccagttt gggaacgctc gaagggttgc
    13861 atcggctttc gcccgacaat gcggaaggtc tgtttttgct cacgaaagga tgggctggcg
    13921 ctggcacgag tttcatcgaa gacgaatggg aaacggccga agacgcgggc aacgagacga
    13981 tggcggaata ccatcggaca cgagcacggg ccgcgtatac acgagcggtt cattatggcg
    14041 tcgagcgatt ggaacagaag gccgatgggt tcgaaagggc gcgacgcaac cctgaggtga
    14101 tgaaacgatg gcttgcagcg ttcgaaaaag aggatgtgcc gtcgctattt tggaccggtt
    14161 acgcttggat tgcgcgtgtg ggtgtctcca aggatattcc ccagctcgta agagaattgg
    14221 gcattggggt agcgatgatc gagcgggtgg tggaattgga tgagacgtac gagcatggtc
    14281 ttgcgcatgt gattctcggc acctttcacg cgcgcatgcc gacagcagaa ctcgacaagg
    14341 cacagcagca tttcgagcgt gcctttcaga tcaaccaggg gcggttgctc atcaccaagg
    14401 ttcagtatgc gcggacgctg tattgttgga aaaaggataa gagcaattat gaaaaattgc
    14461 tgcaggaggt gatcgacgcg ggcgatcctc ttcccaccga acggctttcg aatatgatca
    14521 gccagcgtcg agcccagcgt ttgctttccg acgctcgcat gagtcgatgt ggtttttagc
    14581 gcgggagagt cgggggcgac acgcgttttc gacctcgcga agatccacaa ggtcaagggg
    14641 actggctagc taacgtattg gaatccgagg gatatcatgc atacaaccat cgctatgcga
    14701 tccccggggt cggtcggtat ctttggttgg tcacagagca tccgggcaaa cgggatgtgt
    14761 ttgtggtcaa gggcgtgagc atacagagcg gatgtcgttg acgaagttgg agattgatcg
    14821 gtgaaggata tgtggtgagg ggggcgtgtg aggggctcag gagacgacgg gcaggtgttt
    14881 attttttccg ccggatcggt cgattgaaat cacggtactg cttcgttcgg cgtggaaagc
    14941 ggcgacccgg aaagcgaggg cttcggcggc atcgcgaagg gccagagccg aggggctttc
    15001 gggggaagcc atgacgacgg gggtgccggc atcgcctgct tttcggcatg cggtatccat
    15061 tgggatttga gcgagcaaag gagcgttggc catctcagcg accttggtac cgccgccttt
    15121 tccgaagatt tcgtggcgtt tctcgcaacc atcgcagatg aaatagcttt cgttctcgac
    15181 gatacccaag atggggatgt tcaccttttg gcacatggat acggatttat agacatcgaa
    15241 gagggcgaca tcttgcggcg tggtgacaat cacggcgccg gtgacttgaa gcttttgcga
    15301 gagggtcaag gggatatctc ccgtgcctgg gggcaggtcg agaaagaggt agtcgaggtc
    15361 gccccaatgc acgtcttgaa ggaattgcat gagggcccca tggagcatgg gaccgcgcca
    15421 gacgacggct tcacgggggt catcgagcaa gaatgcgtcg tgcccatggg gcgcgtcgat
    15481 ctcggcgtag ctcacg
//
LOCUS       DAOSOR010000054        15474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_3834_length_15474_cov_4.249043, whole genome shotgun sequence.
ACCESSION   DAOSOR010000054 DAOSOR010000000
VERSION     DAOSOR010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 15474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15474
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3834_length_15474_cov_4.249043"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(403..771)
                     /locus_tag="PK710_04670"
     CDS             complement(403..771)
                     /locus_tag="PK710_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09039.1"
                     /translation="MAEEDDSGGKRGESSASDVVLLHGRTRDGEGIRVLRARDGSVEA
                     GELRAMKQGVPLASGEIVSLTQRSEHPLLWDVKVQHRVESVEHSGPVRVTSSAYRENW
                     ENVFRSKQSRGGMEEDSTLN"
     gene            854..1339
                     /locus_tag="PK710_04675"
     CDS             854..1339
                     /locus_tag="PK710_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09040.1"
                     /translation="MSPKRRDLFRWLVASGTVLAIGNFPLYAWSKPKPKTTIHWTNVE
                     FPEDKLRTQRERILRTILRKESSTEDWGPHNQRIEASIRIIEYQIVHTPEVVRVTCTA
                     VGKLVGGPSVRTHFSIGDHPTKQAKLERNMMTFVARGIVTRLAAIARNRAAAKAKNDD
                     A"
     gene            complement(1350..2327)
                     /locus_tag="PK710_04680"
     CDS             complement(1350..2327)
                     /locus_tag="PK710_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09041.1"
                     /translation="MPKTQYNSVPDREGLRFSPAREGEDGLEANYRPSEHGNAWPNGE
                     EALAAAEKRPGNAMAATSDDWVQRFSEDTEQRLALGNGDDEGNMRGEQRSISLLPPMT
                     NASRMLLLPPRKRFDSLHSSSLFPSPTPLPRFSLGPRMKSLVGSGRAVTSSTTSSTVP
                     SSLAPIAADDRASGQFHRNPWMTGLIGLALAVAAGIALLWVPASLTSYAVDRQLDTPL
                     RALVPVATEAAATESGANAFDERLASEIVMLAARQASGCVQGVELKIRLTFWPDGGLS
                     YQVLDAVGGPPSARTCVDAAFFGLRTAAFDGMVQTRLLCLPELSLAVEP"
     gene            complement(2455..4056)
                     /locus_tag="PK710_04685"
     CDS             complement(2455..4056)
                     /locus_tag="PK710_04685"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOT09042.1"
                     /translation="MRRLHHSTEILRMIGAFVALAVGLTPACKRTSAPADEPDAAGFA
                     TTTSEPVSSVVPSATSDSEPDLAPSIPKTADESAQVSSEYAWLPARTVTAGSPHKIAA
                     KAMQAWVHQYPDDETPYLGYIRAGRIVDRSESWIAKTVRCPDGWYEVLPEGYICRGNR
                     ATLDMNDPVVVASWKQPRRGEPLPYRYVRPASLQTYLYFTLPSKKDQARTEGADMEAY
                     VAARPTERLPNIDVLGEPDPIPPFLADGQYLHTPFGATKRLRYNVHEGRANPQAAFAI
                     MSVHDFEGRRFGLTTELDLIAIDRTKIVRPSSRTGSEVEDLPAGIVRALSIPRYTLDS
                     DGNPVKDGSFSQYQILDLTGNTHRHLWQVRDGSYVAAGAVQMIEPRTSFPSFAVDERK
                     WIDISIKNQLLVAYRGKRAVYVAQVSTGLGELADASKSFATIQGSFTIKSKHVTATMT
                     GNRQADGYELADVPYVQYFHEGYALHAAFWHDNFGRTQSHGCVNLTPADSAWIFEFTD
                     PPVPEHWHGVVGHEGTPRTVVHVRP"
     gene            4055..4180
                     /locus_tag="PK710_04690"
     CDS             4055..4180
                     /locus_tag="PK710_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09043.1"
                     /translation="MAQRNARCREQGRSQEQQADGAFSKDAPGEMGNGKILRPRD"
     gene            4281..5711
                     /locus_tag="PK710_04695"
     CDS             4281..5711
                     /locus_tag="PK710_04695"
                     /inference="COORDINATES: protein motif:HMM:NF037415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09044.1"
                     /translation="MRQLKQLMEEITRRVDTNLREFDMDSSNYIRSALPMSHFMRFYA
                     FYGITSNHPIDFHFGRSSLAGSYFLSRCHVNSSILHKTDVRGDELKRRGQVFHLGGRE
                     ILMHQDECIRVQHSFLVKTLVHSYNHDPEFPEFYSIVNTFAAPYANIHGSAIRGCLLG
                     PFATLDLTTVHDCMIGPFAYVQTGQISHTAVEPGSVWIEHGSDFRFHYQFPKDALQEY
                     VRYDAKRGVQGRLVAFIRQRKRHFQEIFDVVHFNRSDNTHRSTALNRYAVVRGGTRIS
                     ENVLVAQRAYLENADLGKGANAQENCYIVDSCLQGFNVMAHGAKIIHARLGEKGFVGF
                     NAFLRGSPQAPLDIGHHSIVMPHTIIDTETALQIPPEHLVWGLIRNPQELAENSISLE
                     KLSQHNQGFRQGRLVFDGSGKTFVEQFQKRIEHILLDNGAYFDGRQKRGHAQQGQNIA
                     FNIIQPYTTGPRRGLFPTMNIHEGTG"
     gene            complement(5715..6242)
                     /locus_tag="PK710_04700"
     CDS             complement(5715..6242)
                     /locus_tag="PK710_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOT09045.1"
                     /translation="MLNQIGKKQAPRPDLVRNLLDCHHRIRYFSGLAGKLADTEGVPD
                     EEVQSTALAIARYFRESLPIHAIDEEKSLLPRLVGKNQQLDEALERMAIQHDEHEPML
                     TELLQMMGELAEQPSTLKQLRPALSSLAERLQGAFALHLEQEEHMIFPALTTALGAEQ
                     HNEIMLEMTARRQRG"
     gene            6350..8281
                     /locus_tag="PK710_04705"
     CDS             6350..8281
                     /locus_tag="PK710_04705"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOT09046.1"
                     /translation="MAQPIAHRDAPAHNQVMKLASTLLPFALSSALFVGAFVACGSDD
                     ESSGASSKGPPDAQVQQDAPSDHVGEAGSEAAEVEPGSLDSDGDGVPDSDDICPGFDD
                     AIDTDGDGIPDGCDHCPHGDDRLDADGDGIADECDICPGLDDQMKSEANGTADCRNVI
                     KLWLRADRGVFTDDGSGASGTQAKDGERVRLWEDQGEAKNHAQQAISNRQPVFMASAL
                     PNGGSAIKFEGVHTPNDGWSNDLEGPLSLTNRAGKSVFVVARASHANPTPLFELNRLA
                     TVAGSTFQLTTRIGVDVQNGFAWFSDQPLGRRFHIIAVQHPNQASTSDIRAWYDGIPI
                     DASNVQPCDIDTGSGGYRVGGGHALAEAGFSGDIAELLVYEHVLTPKQREAVGFTLER
                     KHGLKTWYVEQNSPVSVYLLAGQSNMVGQGYGGELSEPLNDPQGDVMFWWGNHSGWSP
                     LRTGSGNGPDQFGPELNYGRLMADAKPGSRLVLIKHAVNGTSLAQDWNPSSGPIYAGF
                     MKTLSEARARLEGLGIEYVIRGMLWMQGESDAMQEAMAASYLNNFLDFATAVRTATGV
                     ANLPIVMARIRATMPAPFPYALQVRMAQETAATMDPNVHVIDTDSLTFHGDDVHYDTA
                     GQLELGARFAQAMLTYAPP"
     gene            8438..10237
                     /locus_tag="PK710_04710"
     CDS             8438..10237
                     /locus_tag="PK710_04710"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOT09047.1"
                     /translation="MNSARSRLTSLRKAMREHGVDAYLIPTADAHLNEYVPTCWQRRN
                     WLSGFTGSAGDLLVLPNQAALWTDGRYFLQAEQELEGSGIELYRSGEPGVPTIEEHLC
                     SVLKKGKKVGIDPRVLSMARAKAMREKLSKAGVILAFVDTNLVDSIWDGAPCMPRAPI
                     EIVPLQRAGESTESKLNELRSAMRKKGADALVITTLDAVAWTFNIRGKDVDYNPVVIA
                     YGLITLEEATLYVDPSKVGKAMAEKLQGMTICPYEQMGAALESLAKRKACVWVDQDNT
                     NQWVAEQLGPCSLVTDASPIIERKARKNPLEIAGMRRAHLRDGVAMVKFLRWLSEQVP
                     KGNVTELSAERQLEVFRSQGRHYRGPSFRTIAGFGPHGAIVHYAATEQSNAKLRSGIF
                     LIDSGGQYLDGTTDITRTVQLGSKATAEHRRVFTLVLKGHIALARAKFPTGVRGMRLD
                     TLARLPLWMDGRDYNHGTGHGVGAYLSVHEGPQSISPVRCQGAVLEPGNILSNEPGYY
                     AAGKFGVRIENLVLVVKDPKLSTSEKTWLMFETLTLCPIDRTLLDMSLLTPEEIAWLN
                     EYHRRVESKISPLLDDDRDRRWLEKACAPISKD"
     gene            10496..14791
                     /locus_tag="PK710_04715"
     CDS             10496..14791
                     /locus_tag="PK710_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09048.1"
                     /translation="MHRYLFILLATAVGFGCASTPPVAPSSLNTRCQPTDLASCEAGL
                     KQTLLAATPDTWTQAYVEARAVALGSQDPWVTLHHTFRQRGAGTVVLMVPAQQKQALE
                     VAVRHELKALGSDNVALSVIEQPRLPKLQHISEQEMMFALARVGKVEQVLWFQDDALA
                     RVLGTDLLSPLVFQLPAIMKDRWPTQASANLARPLAEAIAWEHLTNDALQMLGHGAYE
                     KAASLVFRLDTMLTDVPTNRTFALRARRLLSHAHAFGASLPKRKNSESKEQTSTIGDV
                     DVTDQGAYSAWLRISLADKDGTAAWQKHRAVVASACTAERTALLDWFYSYDSTTGRKQ
                     CFSEGQPIPEAALYGDPLFAIQLAQRARQGTISALAWADAYEALVERTETQGLGWYVL
                     PQLLFERGELPGMSARGSKTYTRVTRLAVDHFHALGRFQQSTPEKYSPTGQLTFALLS
                     AALSDPEIVNALHPLLADNIKYELTTATTSSELLETTVVLAVMGAMLPDPFRAASLGF
                     LQRELDNKLHSGFDKSGGWGAAALYLANGFGAYALGNKPNMAFTGDGIIHALESESTE
                     GRPLALVTARGVQYATLAATGELEPEKIGGAKVGEKRAAARARLDQALRSMAEGSEPI
                     PNVAQGHLTTLIDGLIATSALMFQHSSKRDDHACATHESARSLASVQKNLKVLTASRN
                     ALMRDPALRAGASAWARRAALLSVIASDVLDWMATSAGASPKFQWPSENAKTAIAAAL
                     SEWTRRDVAVGIASLHAIARSVAATDGNWESTLDTAKADLVNVLTGTTGLMGTQSSLL
                     TRAFLQEATRIFSETDTRSLSESSSKLVDIADALFKTGQKDQASMVLLLSGALSHDAA
                     TTQRAAGIAVEQKDASAWYLLTHGAVQRTTVDSALDAALYERGLRAVTDDACTIVQLD
                     PLAQVAKAVQGYAQGEGMAAVDKLHRVLEVIENKGLYVPRVQYRLHDPFAGDRVLRLE
                     MYMTTGQGFIAENSFSLGVDVFSSASVDGLRVEVLPAVDEDAGRYYAHIASLAAIYDW
                     LEARDDAGSHNAERMLNAVVLGVRLGHHGVKPTDPKKWISDLDPSLQVAAELASRRGH
                     ILLAGSLWSMALESKDKRPADKPFSPPEAWKDDELPVGLRALARVRDLKNAVYETQSL
                     IEQASSCDVKQRPIAPKNLNSCESYRRAIALWRAGVYESPPTMREGAAEACTREAILV
                     GLLQDISKNSPFAPRIQDAIVDYISTATQRGHDYDALMLLRAMRDRGICDPRLVQLAR
                     RIGSKRTLTPHARVDWVGYSLTCPGRNADDTIWQDLRLLAESSEQIGDAQISQRIYSA
                     IVRYMVNLRAWDRLEAFSRDEGFVRAMNQTGPAGAATSLLIQHTAATLAGLKPDPSKS
                     LGTYEAACVRFGTANREIICRELSTFRNRLTREAGVDHGPEAERLLKQLTGVE"
     gene            14890..>15474
                     /locus_tag="PK710_04720"
     CDS             14890..>15474
                     /locus_tag="PK710_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT09049.1"
                     /translation="MNPRAHAHGGEESIRSYYDEFAVRYEDQRRTRRPNGYHALIDDL
                     EIDFALRYATGKDLLEVGCGTGLILERLAPHCKTAKGVDLSPGMLQPAQARGLDVQEG
                     SATSLPFEDETFDVVCSFKVLAHVSEIEKAFREMLRVTRKGGYVLAEMYNPLSLRALA
                     KRLGPAGFISDKTKESAVFTRFDSPWKTKKLIPAG"
BASE COUNT         3430 a         4479 c         4279 g         3286 t
ORIGIN      
        1 aatggtgaac gccgtgatga gaatatataa aagcaacgca aaaccttcgg tggctggtta
       61 gtgtcgggat tttgtcgctg cggtcaagtc gatcgcttca tttctggcac cgagaatacc
      121 catcgcctca atcccatctc gaaggaaaga attcagggcg acaagtcccc ctaccataac
      181 gcacgagcag ggaagaacaa gcggctgccc tcgaacttcg acagccacga gcccaggcac
      241 ccatttcctt ccgatgtcct catgaaaacg agggcagaac gcgtgccacg gaggcgattc
      301 gttccatgag ccaacgcaaa atgcctcacg attcgatacg ggacacgcag cattgctcct
      361 catcgctatc cggatccgga taacccgaag atgccttttt tcttaattca acgtcgagtc
      421 ttcttccatc ccaccccttg attgtttcga gcgaaatacg ttttcccagt tttctcgata
      481 ggcagaagaa gtcacccgta ccggacccga atgctctacc gattccaccc tatgttgcac
      541 tttcacatcc cagagcaacg gatgctcgga tcgctgggtc aaagatacga tctccccgga
      601 ggccagcggc actccctgtt tcatcgcccg caattccccc gcttctaccg aaccatcgcg
      661 cgctcgaagc acacggatcc cctccccatc ccgcgtacga ccgtgcaaca acaccacatc
      721 agaggcactc gattcaccac gtttcccacc agagtcgtct tcttcagcca tgaacacctg
      781 ttgtacacca acttccaaag ataaccatgc cggaaactag ccaccgatcc caagccgacg
      841 ctactgtcca tgcatgagcc cgaaacgacg cgatctattt cgctggttgg tcgcctccgg
      901 aaccgtcctc gccatcggaa atttccccct atacgcctgg tccaaaccca aacccaaaac
      961 caccattcat tggaccaacg tcgagtttcc cgaagataaa ctgcgaacac aacgcgaacg
     1021 catcttgcga accatcctgc gaaaagaatc atcgaccgaa gattggggcc cccataacca
     1081 acgcatcgaa gcttccattc gcatcattga gtaccaaatc gttcacaccc ccgaggtcgt
     1141 tcgcgtcacc tgcaccgccg tgggaaaact cgtcggtggc ccttccgttc gaacccactt
     1201 ttccatcgga gaccatccca ccaaacaggc caaactcgaa cgcaacatga tgacgttcgt
     1261 cgctcgcgga atcgtgacac gcctcgcggc cattgcccgc aatcgagccg cggcaaaagc
     1321 caaaaacgac gatgcctgac ggcgctacct cagggctcga ccgccaacga tagttccggc
     1381 aaacacaaca acctcgtctg gaccatccca tcaaacgccg ccgtccgtag cccaaaaaac
     1441 gcagcatcca cgcaagttcg cgccgacggc ggccccccta ccgcatccaa cacctggtac
     1501 gacagccccc catcaggcca aaacgtaagc cgtattttca attccactcc ctgaacacac
     1561 ccactggcct gcctcgccgc cagcatcaca atctcgctgg ctagacgctc atcgaacgca
     1621 ttagcgcccg attccgtcgc tgccgcctcg gtcgccacgg gaaccaaagc ccgcaaaggc
     1681 gtgtcaagct gccggtccac cgcataggat gtcaggctcg cgggcaccca caaaagagct
     1741 atccccgcgg caaccgcaag cgccaaacct atcaatcccg tcatccacgg attgcggtgg
     1801 aattgtcccg atgcgcgatc gtccgccgcg atgggagcaa gcgacgacgg taccgtcgaa
     1861 gaggtagtcg aagaggtaac ggcacgaccg gaacccacaa gggacttcat tcgcggaccc
     1921 aacgaaaaac gcggaagtgg cgtaggcgat gggaataagg aagaactgtg gagagaatcg
     1981 aagcgttttc ggggaggtaa tagcagcatt cgcgacgcgt tcgtcatcgg tggcagaagc
     2041 gaaatcgatc gctgctctcc gcgcatattc ccctcgtcat cgccattgcc caacgccaat
     2101 cgttgctcgg tatcttcgga aaaccgctgg acccaatcgt cagaagtcgc tgccatcgcg
     2161 ttgcctggtc gtttttcggc agcagctaaa gcctcctccc cgttcggcca agcgttgccg
     2221 tgctccgacg gtctgtagtt ggcttcgagg ccatcttccc cttcccttgc aggagaaaaa
     2281 cggagccctt cgcgatccgg taccgagttg tactgggttt tgggcattaa cgactccgat
     2341 gagcaagtca gggcgaccga tacaatatct tacctaacga caggtatgat actgtcttac
     2401 ctacggtcaa gctcccctct ttcggaaaac agcattcggt gagtcgaaat gcgttcacgg
     2461 acgaacgtga acgaccgtgc gcggggtgcc ttcatgcccc acgaccccat gccaatgctc
     2521 ggggacaggc gggtccgtga attcaaaaat ccacgcagaa tctgctggcg tgaggttcac
     2581 acaaccatgg ctctgggtgc gtccgaagtt gtcgtgccaa aacgccgcgt gcaacgcata
     2641 gccctcgtga aaatattgga cataagggac atcggcaagt tcataaccgt ccgcctgacg
     2701 attccccgtc atggtcgccg tgacatgctt ggacttgatg gtgaatgagc cttggatggt
     2761 ggcaaagctt ttggaagcat ccgccagttc gcccaaccct gtcgatactt gcgccacata
     2821 caccgcccgt ttcccccgat atgccaccaa aagttggttt ttgatggata tgtcgatcca
     2881 cttgcgttcg tcgaccgcaa aggatggaaa cgacgttcgc ggctcaatca tttgcacggc
     2941 tcccgcagcc acatacgaac catcgcgcac ttgccaaaga tgacggtgcg tgttgccggt
     3001 caagtccagg atctgatact gactgaagga tccgtctttg acaggattgc catcgctgtc
     3061 gagcgtgtag cgaggaatcg acagagcacg aacgattccg gccggcaaat cctccacttc
     3121 gctaccggtc cgagaggaag gacgaactat tttcgtgcgg tcgatcgcaa tgagatcgag
     3181 ttccgtcgtg agcccgaagc gacgaccttc gaagtcgtgg acggacatga tggcgaaggc
     3241 ggcttgcgga ttggcccggc cttcatggac gttgtatcga agccgcttgg tggcaccaaa
     3301 cggcgtgtgc aaatactgac cgtccgccaa aaagggcgga atgggatctg gctccccaag
     3361 aacgtcgatg ttgggcaacc gctcggtggg gcgcgcggct acatacgctt ccatatccgc
     3421 tccctcggtg cgggcctgat cctttttcga aggaagcgtg aagtacaaat aggtttggag
     3481 ggacgcgggc cgaacgtagc gataaggcaa cggctcacca cgtcgaggct gtttccaaga
     3541 cgcaaccacg acaggatcgt tcatgtcgag ggtggcccga tttccacggc aaatatagcc
     3601 ttccggcaat acctcgtacc aaccgtcggg acaacgaacc gtcttggcga tccaactttc
     3661 ggatcgatcg acgatacgac ccgcacggat atacccgagg taaggggttt cgtcgtcggg
     3721 atattgatgc acccaggcct gcatggcttt ggccgcgatt ttatgagggc taccggccgt
     3781 aaccgttcgc gcgggaagcc aggcatattc cgacgacacc tgtgcggatt catctgcggt
     3841 tttgggtatc gacggcgcca aatccggttc ggagtcagac gtcgccgaag ggaccacgga
     3901 actcactggc tcgcttgtgg tcgtcgcgaa accagcggca tccggttcgt ccgcaggcgc
     3961 tgacgtccgc ttacacgccg gtgtcaaccc caccgcaagc gccacgaaag caccaatcat
     4021 tcgcaaaatc tctgtgctgt ggtgcaatcg tcgcatggcg caacgaaacg ctcgttgtcg
     4081 agaacagggt cgaagtcaag agcaacaggc cgatggcgcg ttttcgaaag atgctcctgg
     4141 cgaaatgggc aacggtaaga ttttgcgccc gcgggattaa tttctcgttt gcggctgcca
     4201 agccgcaatg ttagcgaaaa gaaatttcgc atatccccgc aaaacatgcc gcttctgcct
     4261 acccatcaag gagagtcgta gtgagacaac tcaagcaact catggaagag atcacccgac
     4321 gggtagacac caatttgcgg gaattcgaca tggattcgtc gaattatatt cgctctgccc
     4381 tgccgatgtc ccactttatg cgattttatg cattttacgg aatcacttcc aaccatccca
     4441 tcgattttca ctttggccgt tcgagcttgg caggaagcta ttttttgagt cgatgtcatg
     4501 tcaatagttc catcctgcac aaaaccgatg ttcgcggcga tgaactcaaa cgtcgcggcc
     4561 aagtatttca tctgggtggg cgcgagatcc tgatgcacca agacgaatgc attcgtgttc
     4621 agcattcctt tttggtcaaa acactcgtcc actcttacaa tcatgatcca gaatttccgg
     4681 aattctattc gatcgtcaat acctttgccg cgccgtatgc caatattcat ggctctgcca
     4741 tacgcggttg tttgctcggg ccttttgcca cccttgacct gacgacggtt cacgattgta
     4801 tgatcgggcc atttgcctac gtgcaaacgg ggcagatctc gcatactgcg gtcgagccgg
     4861 gcagcgtatg gattgaacac gggtcagatt tccgttttca ttatcaattc ccgaaggatg
     4921 ctcttcagga atatgtccga tacgatgcca agcgcggggt tcagggacgg ttggtagcct
     4981 tcattcgcca gcgaaaacgg cattttcaag agattttcga cgtggttcac ttcaaccgtt
     5041 ctgacaatac ccatcggagc accgcgctga accggtacgc cgtggtacgg ggagggaccc
     5101 gtatcagcga aaacgttctg gtggcccagc gcgcctatct cgaaaacgcc gatctcggca
     5161 aaggcgccaa cgcccaggaa aattgctaca tcgtcgatag ttgcttgcag ggattcaacg
     5221 tcatggccca cggcgccaaa atcatccatg cgcggcttgg ggaaaagggt ttcgtgggct
     5281 tcaacgcgtt tttgcggggt tctccgcaag ctcccctgga catcggtcac cattcgatcg
     5341 tgatgcccca caccatcatc gacaccgaaa ccgcgttaca aatcccaccg gaacacctgg
     5401 tctgggggct catacgcaat ccgcaagaac tcgcggaaaa cagcatttcg ctcgaaaaac
     5461 tctcccaaca taaccaggga tttcgtcaag ggagactcgt tttcgatgga agcggcaaga
     5521 ccttcgtcga gcaattccaa aagcgcatcg agcatatcct gctggacaac ggcgcctatt
     5581 tcgatggtcg gcaaaaacgc gggcatgcac aacaagggca aaatattgcg ttcaacatca
     5641 tacagcccta caccacgggt ccgcgtcggg ggctatttcc aaccatgaac atccacgaag
     5701 gaacgggcta atcgtcatcc ccgctgtcgc cgcgccgtca tttccaacat gatttcgtta
     5761 tgttgctccg ctcccaatgc cgtggtcaac gcgggaaaaa tcatgtgttc ttcctgctct
     5821 aggtgcagcg caaaggctcc ctgaagccgc tcggccaggc tggataacgc tggccggagt
     5881 tgctttagcg tcgatggttg ctccgccagt tcacccatca tttgcagcaa ttcggtgagc
     5941 atcggttcat gctcatcgtg ttgtatggcc atgcgttcga gcgcctcatc gagttgttgg
     6001 ttttttccca caagacgagg aagcagactc ttttcctcat cgatcgcatg gatcggaagc
     6061 gactcgcgaa agtagcgggc aatcgccagg gccgtggatt gaacctcttc gtcgggcacg
     6121 ccttcggtgt ccgcaagttt acctgccaag ccagagaaat agcgaatgcg gtggtgacaa
     6181 tcgagcagat ttcgaacgag atccggacgt ggtgcctgct tttttccaat ttgattcagc
     6241 atgatgggtg acgggaccat gttcccggat catcgaatgt caagcgtcgt tgctccgaaa
     6301 gaattcacgt ttcgatgcat gggccctcgg aaacgttgca tcatgggaca tggcacaacc
     6361 cattgcgcat cgagatgctc ccgcgcacaa tcaggtcatg aagctggcgt cgacgctctt
     6421 gcctttcgcc ctttcgtccg cgctttttgt tggtgccttt gtcgcttgtg ggagtgacga
     6481 tgagtcatcg ggggcctcct cgaagggccc tccggacgcg caagtgcaac aggacgcgcc
     6541 atccgatcac gttggtgagg ctggttcgga ggcggccgag gtggagccgg ggtccctcga
     6601 ttcggatgga gatggggtac cagacagcga cgatatttgt cctggtttcg atgatgcgat
     6661 cgacacggat ggcgatggga ttcccgatgg ttgcgaccac tgcccccacg gcgatgatcg
     6721 tcttgatgcc gatggcgatg gaatcgcgga cgaatgcgat atttgccctg gtcttgacga
     6781 tcaaatgaag tcggaagcaa acgggacggc ggattgccga aacgtgataa agctatggtt
     6841 gcgagcagac cgaggggttt ttacggatga cggctctggc gcatcgggca cgcaagccaa
     6901 ggatggcgaa cgcgtacggc tctgggaaga ccaaggcgag gcgaaaaatc atgcccaaca
     6961 agccatctcg aaccgtcagc ccgtattcat ggcatcggca ctgcccaacg gcggttccgc
     7021 catcaagttc gaaggcgttc acacccccaa tgatggatgg tccaacgatc tggaagggcc
     7081 cctttctctc acgaatcgcg cgggaaaatc ggtgtttgtc gtcgcccgag cctctcacgc
     7141 caatcccaca ccccttttcg aattgaatcg tcttgccacc gtggctggct cgaccttcca
     7201 actcacgacg cgcattggtg tcgatgttca aaacggtttc gcgtggtttt cggatcagcc
     7261 cctgggcaga cgatttcata tcatcgcggt gcaacacccc aaccaggcca gcacatccga
     7321 tattcgcgcg tggtacgacg gcattccgat cgacgcctcg aatgtgcaac cttgcgatat
     7381 cgacacgggg tcggggggat atcgtgtggg aggaggtcat gcgttggcgg aagcgggatt
     7441 ttccggggat attgctgaac tcctggtcta tgagcacgtg ctcacgccca agcaacgcga
     7501 ggccgtgggc ttcacgctgg agcgcaagca cggtctcaag acgtggtatg tggagcagaa
     7561 ttccccggta tcggtgtatt tgttggcggg tcaatcgaat atggtgggcc agggttatgg
     7621 tggggagctt tcggagccat tgaacgatcc gcagggcgac gtcatgtttt ggtggggcaa
     7681 tcattcgggc tggtccccgc tgcgaacggg cagtggaaat ggaccggatc aatttggtcc
     7741 ggaactcaac tacggacgcc tcatggcgga tgcgaagccc ggttctcgcc tggttctcat
     7801 caagcacgcg gtcaacggta cgagcctggc gcaggattgg aatccaagtt cagggccgat
     7861 ctacgcgggt ttcatgaaaa cgctttcgga ggcgcgtgcc cgcttggaag gcctgggtat
     7921 cgaatatgtc attcgtggaa tgctctggat gcaaggcgag tccgacgcca tgcaagaggc
     7981 gatggcggcc tcgtatttga acaacttcct cgacttcgcg actgccgttc gtacggcaac
     8041 gggagttgcc aacctgccca ttgtgatggc tcgaattcgt gccacaatgc cagcgccttt
     8101 cccttacgcg ctgcaggtac gaatggcgca agaaaccgcg gcaacgatgg acccgaacgt
     8161 acacgtcatc gacacggatt cgttgacctt ccatggtgat gacgtccact atgatacggc
     8221 cggacagctc gagttgggcg cgcgttttgc gcaggccatg ctgacctatg ccccaccata
     8281 acgtatctca gagtcgagtt actatttacg ctagggaacg ctctggcaat atcgttcggc
     8341 aaatctcgac gctgggggtc tgtcccgtgt acgcttggcg accgtccttg gggaaccccc
     8401 ttcgggacac catccagtgc agtggagtgc gataacgatg aatagcgccc ggtccagact
     8461 gacgtccctt cgcaaagcca tgcgtgagca cggtgtagat gcctatctca ttccgacggc
     8521 ggacgcgcac ttgaacgagt atgtgccgac ctgctggcag cgtcgaaact ggttgagtgg
     8581 ctttacggga tccgcgggag acttattggt tcttcccaac caagccgcgt tatggaccga
     8641 tggtcggtat tttctccaag cggagcaaga actcgagggt agcggtatcg aactctatcg
     8701 gtctggagag ccgggtgtac ctacgatcga ggagcatctg tgcagcgtgc tgaagaaggg
     8761 caagaaggtc ggaatcgatc cacgcgtgct ttcgatggcc cgagcgaagg ccatgcgaga
     8821 gaaactgtcg aaagccggtg tcatcctcgc attcgtggac accaacctcg tggattcgat
     8881 ttgggatggc gcaccttgca tgcctcgcgc tccgatcgaa atcgtgcctc tgcagcgcgc
     8941 tggagaatcg acggaaagca aactcaacga attgcgctcg gccatgcgga aaaaaggcgc
     9001 ggatgccctt gtgatcacca cgctcgatgc ggtcgcctgg acgttcaata tccgaggaaa
     9061 ggacgtcgac tacaatcctg tcgtgattgc gtatgggttg atcacgctcg aagaagcgac
     9121 actttatgtg gatccatcca aggtgggcaa agcgatggcg gaaaagcttc agggcatgac
     9181 gatttgtcct tacgagcaaa tgggcgccgc tctggagtcg ttggcgaagc gaaaagcctg
     9241 tgtttgggtc gaccaagaca acaccaacca atgggtagcg gagcaactgg ggccgtgctc
     9301 cctcgtgacg gatgcaagcc cgatcatcga gcgcaaagca cggaaaaatc ccttagaaat
     9361 cgcagggatg cgccgtgccc atcttcgcga tggcgtggcc atggtgaagt ttttgcgctg
     9421 gctatccgaa caggttccga aaggcaacgt gacggaattg tccgcggaac ggcagctcga
     9481 agtttttcgc tcccagggac gacactaccg tggtccgagt ttccgcacga tcgccggctt
     9541 tggacctcac ggcgccatcg ttcattacgc cgccaccgaa caaagcaatg ccaaactccg
     9601 ctccggcatc tttttgatcg actctggtgg ccaatatctc gacggaacga cagatatcac
     9661 gcgaacggtt cagctcggtt ccaaagcaac ggcggaacat cgtcgcgttt tcacgctcgt
     9721 actcaaaggc catatcgccc tggctcgtgc gaagtttccg acgggagtgc gcggcatgcg
     9781 gctcgatacg ctggctcggc tgcctttgtg gatggatggt cgagactaca accacggaac
     9841 gggtcatggg gttggcgctt atttgagtgt tcacgaggga ccgcagagca tcagtcccgt
     9901 tcgttgccag ggagccgtcc tcgaacccgg caatattttg tccaacgagc ctggctatta
     9961 tgctgcggga aagtttggtg ttcgcatcga aaatctcgtg ctcgtcgtga aggatccgaa
    10021 gctttcgacc tccgaaaaaa cctggctcat gttcgaaacc ttgacccttt gccccatcga
    10081 ccggacgttg ttggacatgt ccttgctcac gcctgaagag atcgcgtggc tcaatgagta
    10141 tcatcgtcga gtcgaatcga aaatctcgcc tcttctcgac gacgatcggg atcgtcgttg
    10201 gttggaaaaa gcttgtgctc cgatttcaaa agactaacgc ctgaacgcat ctcatccatc
    10261 atcgccacgg ggatcgttca tggcattctc gcccctggaa acgccagctt taccactgat
    10321 gatcgaatga tcgcatgctg ttacgggggt gagaaaccgt agcaggccct gcttgcgagt
    10381 ctcctgaacc gacgaatcat ccttgtgccg tgggagtttc gcgccatttg acgcgtcatc
    10441 gtctcgacgc tcttctttct tgacggctgg aactcatcca tggacgcttc gcgtcatgca
    10501 tcgttatctg ttcatcttgc tcgcgaccgc cgttggcttc ggttgcgcca gcacgcctcc
    10561 tgtggcgcca tcttccctga atacccgatg ccagcccacg gatttggcat cctgtgaggc
    10621 gggtctgaag caaaccctgt tggctgcaac gccggataca tggacgcaag cttatgtcga
    10681 agcgcgggcc gtcgcgttgg gttcacaaga cccgtgggtg acgttgcatc atacgttccg
    10741 gcagcgtggt gcgggaaccg ttgtcctgat ggtgcctgcg caacagaagc aagccttgga
    10801 ggtcgccgtt cgccatgaat tgaaagcgtt gggtagcgac aacgtagcgt tatcggtgat
    10861 cgaacaacct cgtttgccaa agctccagca catatccgaa caggagatga tgttcgcgtt
    10921 ggcgcgtgtc gggaaagtcg aacaagtgct ttggtttcaa gatgatgcgc tcgcgcgggt
    10981 gttgggaacg gacctgcttt cgcccctagt atttcagctt ccggcgatca tgaaagaccg
    11041 ttggccgacc caggcttccg ccaatttggc gcgccccctg gccgaggcta tcgcatggga
    11101 acacctgacg aacgatgcgc tgcagatgct tgggcatggt gcctacgaaa aggcggcatc
    11161 cctggtgttt cgcctcgaca ccatgctcac tgatgtgccc acgaatcgaa ccttcgcgtt
    11221 gcgagcacgc cgtctgctgt ctcatgccca tgctttcggc gcttcgcttc ccaagcgtaa
    11281 aaacagcgaa tccaaagagc aaacctcgac gataggggac gttgatgtca ccgatcaagg
    11341 cgcctactcc gcctggttac ggatctcgtt ggcggataag gatggcacgg ctgcttggca
    11401 aaaacaccga gctgtcgttg cttccgcgtg tacggcagaa cggacagcgc tgctcgattg
    11461 gttttacagc tatgattcga cgacgggacg caaacaatgc ttttcggaag ggcagcccat
    11521 tcccgaagcc gccttgtatg gagatccgct gtttgcgatt caactcgccc agcgagcacg
    11581 gcagggcacg atttccgccc tggcctgggc agatgcttat gaggcactgg tggaacgaac
    11641 cgaaacccaa ggactcggtt ggtatgtatt gcctcaactt ctattcgaac gtggcgagtt
    11701 gcctggcatg tcggcccgag gctccaaaac ctacactcga gtcactcgtc ttgccgtcga
    11761 tcacttccat gcgcttggcc gatttcaaca atcaacaccc gagaaatact cacccacggg
    11821 tcaactcact tttgcgttac tgtcggccgc gctatccgat cctgaaatcg tcaatgcgtt
    11881 gcaccctctc cttgccgaca acatcaaata tgagctgacg accgccacga cttcttccga
    11941 acttctcgaa actaccgtgg tgttggctgt catgggagcg atgcttcccg atcctttccg
    12001 tgctgccagc cttggattcc tacaacgaga attggataac aaactgcatt ccggcttcga
    12061 taaatccgga ggttggggcg cggccgcgtt gtacctcgcc aatggttttg gagcctacgc
    12121 gcttggcaac aagcccaaca tggcattcac tggcgacggc atcattcacg ctctcgaatc
    12181 tgaaagcacc gaagggcgtc ctctcgcgct cgtcaccgcg aggggcgttc aatacgccac
    12241 gttggccgcc acgggagaat tggaacctga aaaaataggt ggggcgaaag tgggagaaaa
    12301 acgcgccgcg gcaagggcac ggctggacca ggccttgcga agcatggcgg aaggttccga
    12361 accgattccg aacgttgccc agggacatct cactaccttg atcgacggtc ttatcgccac
    12421 ctctgcgttg atgttccagc attcatccaa gcgcgacgat cacgcatgtg cgactcacga
    12481 atcggcgaga tctcttgcga gcgtgcaaaa gaatctgaaa gtgctcacgg catcgagaaa
    12541 tgctttgatg cgcgatccgg ccctacgagc gggagccagt gcctgggcgc gccgggcggc
    12601 gcttctgagt gtcatcgcat cggatgtcct ggattggatg gcaacctccg caggggcctc
    12661 acccaaattc caatggcctt cggaaaacgc gaaaacagcg atcgccgccg cgctatcgga
    12721 gtggactcgt cgtgatgtcg ccgtgggtat tgcttcgctt cacgccatcg ctcgctcggt
    12781 agcagccacc gatggcaatt gggaatcgac cctcgacacg gccaaagcag accttgtcaa
    12841 cgttctcacg ggcacgacgg gcctcatggg aacacagagt tccctgctca caagagcttt
    12901 cctccaggaa gccacacgaa tattttccga aacggacaca cgctccctgt ccgaatcatc
    12961 gtccaagttg gtggatatcg cggatgccct gttcaaaaca ggccaaaagg atcaggcaag
    13021 catggtcttg ctcttgtccg gtgcgctgag ccacgacgct gcaacgacgc agcgcgccgc
    13081 aggtatcgct gtcgagcaaa aagacgcctc cgcctggtat ctcctgacgc atggcgctgt
    13141 gcaacgaacg acggtggact cggcccttga cgctgctcta tacgaaagag ggctccgtgc
    13201 ggtgacggac gatgcatgca cgatcgtcca gttggatccg ttggcgcagg tggccaaagc
    13261 cgttcaaggt tatgcgcaag gagaggggat ggcagcggtg gacaagctcc accgagtgct
    13321 agaagttatt gaaaataaag ggctttatgt gcctcgggtc caataccgcc tccacgatcc
    13381 gtttgcggga gaccgcgtgc ttcggcttga aatgtatatg acaacgggcc aaggattcat
    13441 tgcggagaac agtttttcgc ttggggttga cgttttctct tccgcatccg tcgatggtct
    13501 aagggtggaa gtactacccg cggtcgacga ggatgctggt cgatactatg cgcatattgc
    13561 ctccctagcc gcgatttacg attggctcga agcccgtgac gacgcgggtt ctcacaacgc
    13621 cgaacgcatg ctgaatgccg tcgtgcttgg cgttcggctc ggccaccatg gagtcaaacc
    13681 cacggatccg aaaaaatgga tttctgatct cgatccgagt ctgcaggtag ccgcggagct
    13741 cgcatcgcga cgaggccata tccttctggc gggcagcttg tggagtatgg ctttggagtc
    13801 gaaagacaaa cgccctgccg acaagccgtt ttcgccaccc gaagcctgga aagacgatga
    13861 gctacccgtg gggcttcgcg cgttggctcg cgtaagagat ctcaaaaatg ccgtttatga
    13921 gacgcaatcc ctgatcgagc aagcctccag ttgcgatgtg aagcagaggc ccatcgctcc
    13981 caagaatctg aattcttgtg agagctatcg tcgcgccatc gccctgtggc gtgcgggagt
    14041 ctacgaaagc ccgcccacga tgcgggaagg tgccgcggaa gcctgcacgc gtgaggccat
    14101 cctcgtggga ctgctgcagg acatctccaa aaattcgccg tttgcccctc gtatccaaga
    14161 tgcgatcgtc gattatatct ccaccgcgac ccaacgggga cacgactatg atgcgttgat
    14221 gctcttgcgc gccatgcgag atcgcgggat ctgtgatccg agactcgtcc agttggcacg
    14281 aaggataggg agcaaacgga ccctcacgcc ccatgcccga gtcgattggg ttggttattc
    14341 tctcacctgt ccaggtcgca atgcagatga cacgatttgg caagacctgc ggttgcttgc
    14401 cgagtcctcg gagcagattg gggatgctca aatttcacaa cgaatttaca gcgccatcgt
    14461 tcgctacatg gtcaacctcc gggcatggga cagactggag gcattctctc gcgatgaggg
    14521 gttcgtacgc gcgatgaacc aaaccggtcc tgcgggagcc gcaacgtcgc tgttgatcca
    14581 acatacggcc gcgacgctgg cagggctcaa accggatcca tcgaagtcgt tgggcaccta
    14641 tgaagccgca tgcgttcgtt tcggcacggc caatcgcgag atcatttgcc gagaactcag
    14701 tacctttcga aatcggctca ctcgcgaggc tggtgtcgac cacggaccgg aagctgaacg
    14761 attgctcaaa caactgacgg gagtcgaata aaaatcggct gcctcttgcc ttctccttcc
    14821 accggagttt cgtcccacca acgtcgatgg acttggcgtt ggttcaggca tcgcgctata
    14881 cggctgcgca tgaatccacg cgcgcacgcc cacgggggcg aagaatcgat tcgctcctat
    14941 tacgatgaat ttgcagtccg gtacgaagac cagcgcagaa ctcgtcgccc caatggctac
    15001 cacgccctga tcgacgatct cgaaatcgac ttcgcgttac gctatgcgac cggcaaagat
    15061 ctattggaag tcggctgcgg aacgggtttg attctcgaac gtttggcgcc gcattgcaaa
    15121 acagccaagg gagtcgacct ttccccggga atgctgcagc ccgcccaagc gcgcggactc
    15181 gacgtgcaag aaggttcagc cacctcgctg ccttttgaag acgaaacgtt cgatgtggtt
    15241 tgttctttca aagtgcttgc gcacgtatcc gagatcgaaa aagcatttcg agagatgctg
    15301 cgggtaacac gcaaaggggg ctacgtcctt gcggaaatgt acaatccgct cagcctgcgt
    15361 gcgcttgcca aacggctggg cccagccggt ttcattagcg acaagacgaa agagagcgca
    15421 gttttcacgc gcttcgactc gccttggaaa acgaaaaagc tcattcccgc aggc
//
LOCUS       DAOSOR010000055        15431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_3851_length_15431_cov_4.235367, whole genome shotgun sequence.
ACCESSION   DAOSOR010000055 DAOSOR010000000
VERSION     DAOSOR010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 15431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15431
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3851_length_15431_cov_4.235367"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="PK710_04725"
     CDS             complement(<1..824)
                     /locus_tag="PK710_04725"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT09050.1"
                     /translation="MSTSRTTARSADTPLQGGTVPSDAPVSATTIPSAEFPDRSATVR
                     RQVSADSLAEVSQGARGLAPGTLVSPYRVVRLVGRGGMGDVYLARDTQLGRKVALKVV
                     RPSVVGNREEVARFLLEARITARFNHPHIVTVYGAGEFEGQPYVALEYLEGQTLRARM
                     DEQRLGVREVMRIGLAVAEALQEAHRHEVLHRDLKPENIYLPRDGRLRVMDFGLAVPL
                     RLPEVLAEARELDELPEGMEQALKKNMDHGGIEGTPLYMAPEQWRGLGCSTTTDCWA"
     gene            870..1046
                     /locus_tag="PK710_04730"
     CDS             870..1046
                     /locus_tag="PK710_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09051.1"
                     /translation="MSVAADHGSDRRGDDRLVCWQGSRITVPGYAHRAFLIGENNRAL
                     PNHHPVKRKAEANE"
     gene            1046..3958
                     /locus_tag="PK710_04735"
     CDS             1046..3958
                     /locus_tag="PK710_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT09052.1"
                     /translation="MGESRRLPLLCLGAASAALQVLAIREGMAVTAGDETVVALLMGI
                     WLIETALGAMGSRTRTARRTTVSAGFTIYGLTVAATLLAARACVGWIPLGQVPSSFSV
                     ALAAIFILAPACFLSGWLYGQLAKPLENTAKIENGGVVSAQAYWLDTLGSAIAGALLS
                     TVILDHLLPFQVASLVSATAFLGAIFLADPHRRGWFVAALLPCAALAIAPVDRLTYRW
                     HAPGQQVLQLASSPRGAVLVTQQTGQQQVMLQRQPILIPADIEGSEQIANISACLHTC
                     PKRILLVGAPPGNLISSLLAHGIDHLTILAGDRVIADVISSWAPDASDGRVRIVPSDA
                     RAWSGIDEEPAFDLLVVNAGLQTSVAEARLYSTSFYRKLAKATASDGLLVVSLPGFAA
                     YATEAERDLHSTVVSTLKEVFPHILALPGDRTLYVASSSRLIDPADVGKTVLLRMKQR
                     GIDSRYLTEAWVNDRLSTERIHQAALWSGRSVRPSTDTHPVIVSAARQATFERLETGL
                     DSLIFLVLSTILMMMAWVDPRSKPVSFAVASTGFSGLALQLTLMLVFQTAVGALYRDV
                     ALVTGLFMGASCAATVVGMRRAARFRALWLMDAWQLGVAMALAVGVDAMMQAGADLTR
                     AGVIVAALLMGAATGGQFAVASRMDGVFPTGVGASVYGLDLAGAALAAFVSVTWLIPT
                     LGISGTLWAVAGCKAVSLVALVTRSRVSAESLSFRMPLPMMLLGGVILLAIQSTHGPF
                     QRFINSDGYRLVAILVLAAAMASAFEPMPWRRWIRQAEHRSRVFAERIGVSPLRLLSL
                     LALLPVAAQPLGRCMFAIPYLFCHVCPRPCIFGVLRRFVIASALVTNLGDLRFCQRVC
                     PLGLAQASVPSTSSLRKLGKASWWLRIPSLALVIWWVFGIDPGPVHEGEGIVQRLYQH
                     ADAVNPWVLWITGGLLLLSFFVRRPFCESICPIGSTSEILVRADRLVRRRRSPQPP"
     gene            4019..4864
                     /locus_tag="PK710_04740"
     CDS             4019..4864
                     /locus_tag="PK710_04740"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT09053.1"
                     /translation="MRLIPRSSSQFNTKFAVELDDGAWVEAVLYRKHTLCISSQVGCA
                     VGCPFCASGSRGWMRNLHAEELIAQVQQAQSWAPSLRRVTVSGVGEPLHNAAAVETLL
                     HWCRQHHLAPSLTTTGGSEEKLKHFMGLPHNGLTLSVHAGTESMRRKLVPHGLSLSRL
                     FDTVEQCVPRLSRSRRRRLSLAYLLIGDGNDDAEQTGAFSERARSLGLSVYLYRFNPV
                     AHGNFLPAAEKRYEEVVEAWRAMGLEVRCSSLARNEPNGGCGTLMAQAGRVFDDGSGN
                     ENQLG"
     gene            complement(4915..5466)
                     /locus_tag="PK710_04745"
     CDS             complement(4915..5466)
                     /locus_tag="PK710_04745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOT09054.1"
                     /translation="MDDLAVAVPLMEGFEEIEAVTIIDVLRRANIKVIVAAKAPGLVT
                     GAHDIGVQATDTLRDLRPESLAAIVLPGGMPGAKNLANDERVQWLVEKMAQEGKLVAA
                     ICAAPMAIASTKAHLGRKMTAYPGFETYLEGATVLSDRVVVDANMVTSRGPGTALDFA
                     LTLVEKLRDQKTALDLRKKMLVG"
     gene            complement(5738..6097)
                     /locus_tag="PK710_04750"
     CDS             complement(5738..6097)
                     /locus_tag="PK710_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09055.1"
                     /translation="MLSRRAQLLLCTLLSSVLILGCQNDTPTGQITPGKVQAKVGDTV
                     ALSLSVPKKFDRLQREIWKVEPANLGEVYFDQASLTRREATFRAKEAGTGTIVVQGFL
                     TDPKQLHHVAQIPVTVE"
     gene            complement(6223..7215)
                     /locus_tag="PK710_04755"
     CDS             complement(6223..7215)
                     /locus_tag="PK710_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09056.1"
                     /translation="MTSPNFVDTVLLLALPASGKSEIRRYMMHVDRAKRIEQFHLADT
                     VQLDDYPYVELMREIDDALEELGEARRFFKSADDGFQYGHDWGTLLQLVNEDYRVMKN
                     PDLPSPKADAAVMFARIDAARAKVGVPAAFESMSADLRKRLGDRMQKKVEWVVHELFG
                     KRPNSLENKTIVIEFARGGPQGSTMPLQAPHGYQYSLAQLAPEILEKAAVLYVWVEPE
                     ESRRKNLARAVPNAENTILFHAAPESVMINDYGCDDMAYLMETSKVPNTITIHAGGKD
                     YFLPIGRFDNRVDKTTFVRDEPSSWDPKLVAELHAGLADGLSKMWSAHKTVRKL"
     gene            7609..8352
                     /locus_tag="PK710_04760"
     CDS             7609..8352
                     /locus_tag="PK710_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT09057.1"
                     /translation="MNLFRAGKNWWHVGGRAIVYGSISVTAGTITKRHDIGQRCMRAW
                     CQGSLDGLGIQHSIRNGSLLDSATPCVFVANHLSQLDILLIGSYLKQDYRWLAKASLF
                     HIPFIGWHLSVSGHVPVYRGEHKDKNEMLAQRIHDVVVQGASLLFFPEGTRSRDGRLH
                     SFRIGAFRTAVRENLPIVPLVVRGTHELFVPGSKDLAVQAERKCSLTVLPAIHAPSDG
                     DEKSRAAELCERTHAAFVAELTAPSSETQ"
     gene            complement(8356..8895)
                     /gene="cysC"
                     /locus_tag="PK710_04765"
     CDS             complement(8356..8895)
                     /gene="cysC"
                     /locus_tag="PK710_04765"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HOT09058.1"
                     /translation="MTGTSHGLVVWLTGLPAAGKSTLALALRDSLEKAGQAACIIDGD
                     VLRQGLCSDLGFSAKDRQENVRRAGEIAKIIAEQGIIAIVALVSPYERDRRAVQERLP
                     PHRFFEVHVDCSVEECRRRDPKGLYRRADRGQIPQFTGVSDPYEAPSKPELRVRTQDD
                     PVQVSLDQLLAAVTEAIKR"
     gene            8987..9394
                     /locus_tag="PK710_04770"
     CDS             8987..9394
                     /locus_tag="PK710_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09059.1"
                     /translation="MRDRDPRRLEQNLLRDPRRDPTVPPPTAEQDAPATPIIVEAAAE
                     SPPPPKAEPPSSRRGNLDATDPGADRPSSPQPEGASKITSPDPRIAELEPLLASGQWE
                     GIVKYFGSRGKRRKTATQPRAHLRHCTQRIRNG"
     gene            9441..9680
                     /locus_tag="PK710_04775"
     CDS             9441..9680
                     /locus_tag="PK710_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09060.1"
                     /translation="MAIRCIAGLYGISPDSPIAIVLGKRIIRANPVSWQKKKAPPAPI
                     SFLIVALGLLLGAGIGWLLSFGSVTLKLPNLLFLH"
     gene            complement(9831..11738)
                     /locus_tag="PK710_04780"
     CDS             complement(9831..11738)
                     /locus_tag="PK710_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOT09061.1"
                     /translation="MNESDHVSQHTCSFSLHGANPHLETWVRQRVHPFDPSTDDYHRP
                     PFSAQIQTTKQSAIYALHGYHLGKKPHDAIRAYVAHFTAPGDVVLDPFAGSGSTALAA
                     LQEHRHAIGIDASPAATFIARAYLFPIDINDLLARCEAMVQRAADELQWLYATRCHRC
                     GGKAMLHHLVYSQTYACPRCKEITSLFEALQQEGPSCCPSCLRQGKRVRIHSSLDRLG
                     LVPVGVSFSCLEGCHPKRSTRGIVGTREEREAFDRIDLAGIACLERLPIPHPIPDVPM
                     MNRIDDGPWGDEWRPSRNFRCIRDLFTHRNLWALAALWEAADGDLDLQAVLTSAMLAV
                     SRKAQHLEGGGGYIPGHWALPPMTKQRNVLETVRRVFGRSLVARQQLQRLGLRGDVCL
                     STQSCTDLSAIPDASIDYIFTDPPYGGMVQYGELNFLWEAWLGLPTDWHRHEIIVNRT
                     RGLSLETWTERMSRALAECHRVLKPNRWMSVCYHDGSKGSWSALQQAIENAGFDAQSV
                     GSATSIDTKSRTYNQYTVDKVNKRDLVLNLCKSPRARVFPAATVVGQDAFDERVREII
                     IAFLKKHPGSSKDRIFDEVVSTLLCTGPMQDHDFERILRQVAEPQGECERVVETGVRR
                     GAHATSWALRP"
     gene            12001..12768
                     /locus_tag="PK710_04785"
     CDS             12001..12768
                     /locus_tag="PK710_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09062.1"
                     /translation="MNRCAPALFAIEHGDLDACRSELRAWCVHVGHLPGVRVGAKAIG
                     ACASTFRSLSCAAWQARRDDPFCDAPGGSLSVDEPCISGYQCASKACYLDAQGVCGVC
                     QPTAGVGDPCRQSMECPATSACVGYHCLKTMALGEECGSGRPCAGQLLCLSGTCQQSP
                     ALHDPCIENTCNNRLATECVQGVCEPLETAATGQACGLINGKTVYCGRGTCSSQGVCV
                     APVPEGRPCQDSSGPTCLQPAVCVDGWCKILQPSMCQ"
     gene            13274..14464
                     /locus_tag="PK710_04790"
     CDS             13274..14464
                     /locus_tag="PK710_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOT09063.1"
                     /translation="MNKPKAASRYHALDGARAFAMLLGVLLHASMPYLFVLFPWAVVD
                     GSRSVSLTLLIYTIHSFRMPAFFLIAGFFARLVLQRDGVGAFVKHRLRRILGPLVVGW
                     IVLYPLVRFLWVWSGVRMVQGADLYQAVVAAFTPPFLGRGLGLIHLWFLYYLLLLYGF
                     AVGIRWVGLKVDRQGKQQQRLDALFLRLLHSRLGIFGLAIPTACALLPMRGWGVDFAD
                     RSLWPRAAHLLFYGFFFLMGWWLHRHPTLLATLRNRWGMHVAISSVLLFPITGLLYWI
                     YSLHYDAGFAFRIVYFLLYATISWGFVLGWLGAFERFFHRPNAVWRYLADASYWIYLV
                     HLPLVIALAILLRSWPIGWYIKLPLVLASSLPLLLGSYHIFVRYTGIGIVLHGPRIKT
                     NKIN"
     gene            complement(14634..15308)
                     /locus_tag="PK710_04795"
     CDS             complement(14634..15308)
                     /locus_tag="PK710_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09064.1"
                     /translation="MSHVPSLRKSRIAFGSLSVFFFSSFLAIGCDQKTAPVEPGPEKI
                     VAIETAPPPSVADTPANSAKEPEQPVASATAVRAPTTTTPPSQGQDTRPEPKSTASAA
                     EEPKKGADASAASYSAWLQSAGPYEAGKQGALTVVLVAKDPYKCNDKYPYKFKLDPPG
                     AGISYPQDTVRGMQVTPKRSTLSVPFVPAQAGPATVSGTLHFSVCTEERCLIEQAKLS
                     VTVNVP"
BASE COUNT         3035 a         4554 c         4429 g         3413 t
ORIGIN      
        1 gcccagcaat ccgtggtggt ggaacaaccg agtccacgcc attgctctgg tgccatatac
       61 agaggcgttc cctcgatccc accgtgatcc atattttttt tgagcgcctg ctccatgcct
      121 tccggcaact catccagttc cctcgcctcc gccagcactt ctggaagacg caaaggaacg
      181 gccaggccga aatccatcac ccgcaatcgc ccatcgcgcg gcaaatagat gttttcgggc
      241 ttgagatccc gatgcaacac ttcgtgtcga tgggcttctt gcaacgcttc ggccaccgca
      301 agaccgatac gcatcacctc tcgaacccca agccgctgct catccattcg ggcgcgaagc
      361 gtttgaccct ccaggtattc gagcgccaca tacggttgcc cctcgaattc accagccccg
      421 tacacggtaa cgatatgggg atgattgaat cgcgccgtaa tgcgagcctc caacagaaat
      481 ctcgccacct cctcgcgatt gcccaccacc gaaggccgaa ccaccttcag agccactttt
      541 cttccaagct gcgtgtctct cgccaaatac acatcaccca tgcccccacg acccacgagc
      601 cgtaccaccc gatacggcga cacgagcgtt cccggagcaa gcccccgcgc cccttgggat
      661 acctccgcca aggaatccgc agaaacctga cgacgtaccg tcgccgaccg atccggaaac
      721 tccgcgctgg ggatcgtcgt agccgaaacc ggcgcgtcgg acggaaccgt tcccccctgc
      781 aaaggcgtat cggccgatcg agcggtagtc cgtgacgtag acatcgaaga acccacacga
      841 tagcggatcc tgccaaggta ggccacgtca tgagcgtggc ggccgaccac ggttcggata
      901 ggcgcggcga cgatcggctg gtatgttggc aagggagtcg aatcaccgtg cctggatacg
      961 cccaccgtgc cttcctcatc ggggagaaca accgcgccct acccaaccac cacccggtca
     1021 agcgaaaagc ggaggccaac gagtgatggg cgaatcccgt cgcttacccc tgctttgtct
     1081 cggcgccgcc tccgccgccc tccaagtcct ggccattcgt gagggcatgg ccgtcactgc
     1141 cggcgatgaa accgtcgtgg cattgctcat ggggatctgg ctgatcgaaa cggccctggg
     1201 cgccatggga agccggactc gaacagctcg ccgaaccacg gtttccgctg gtttcaccat
     1261 ctatggcctc acggtcgcgg caacgctgct ggctgcccgc gcctgcgtag ggtggatacc
     1321 tctcggtcaa gtccccagct cattttccgt agcgcttgcc gctattttca ttctcgcccc
     1381 ggcgtgtttc ctttcgggct ggctctacgg ccagctcgcc aaaccactgg aaaatacagc
     1441 aaaaattgag aacggggggg tcgtctcggc ccaggcctat tggctcgata ccctggggtc
     1501 agccatcgcc ggagcgctgc tttccaccgt gatcctcgac catcttctgc cgtttcaggt
     1561 ggcgtctttg gtcagcgcca ccgcgttttt gggagccatt tttctagcgg acccccaccg
     1621 ccgcggctgg ttcgtcgccg ccctcctgcc gtgcgccgcc ctcgccatcg cccccgtaga
     1681 ccgactcacc tatcgctggc atgcccccgg acaacaggtg ttacagctcg cctcgagccc
     1741 tcggggcgcg gtgctcgtga cacaacagac gggtcagcaa caagtgatgt tgcaaagaca
     1801 accgattctg atccccgcgg acatcgaggg ctcggaacaa attgctaata tttccgcatg
     1861 cttacacacg tgtccgaaac ggattctgct ggtaggcgct ccccccggaa acctgatttc
     1921 gtcgttgctg gcccacggca tcgaccatct gacgattctt gcgggagatc gggtgatcgc
     1981 cgatgtaatt tcatcctggg cccccgatgc ttccgatgga cgcgttcgga tcgtgcccag
     2041 tgatgctcgg gcctggtcgg gcatcgatga ggagcctgcc ttcgatttgc tggtggtaaa
     2101 cgccgggctt cagacgagcg tggcggaggc tcgtctatac tcgacatctt tttatcgaaa
     2161 acttgcgaaa gctacggcct ccgacggtct tttggtggtg tcgcttcctg gttttgcggc
     2221 atacgccacg gaagcagaac gggatcttca cagcacggtg gtctcgacgt tgaaggaggt
     2281 ttttccgcat atcctagctc taccggggga tcgaacgctg tatgtggcgt cttcgtcgcg
     2341 gctcatcgat cccgccgatg ttgggaaaac ggttctgttg cgcatgaaac agcgagggat
     2401 cgactcgcgc tacctgaccg aagcgtgggt caatgatagg ctttccaccg agcgcattca
     2461 ccaggcggcg ctgtggagtg ggcggagcgt gcgtccgagc acggacacgc atccggttat
     2521 cgtttcagcg gcgcggcagg ctactttcga gcgtctcgag accgggctgg attcgctaat
     2581 tttcctagtg ttatcaacta ttttgatgat gatggcctgg gtcgatccgc gttcgaaacc
     2641 ggtgtctttc gcggtggcaa gtacgggatt ttcagggctc gcgctgcaac tcacgttgat
     2701 gttggtattt caaacggcgg taggcgcgct gtatcgcgat gtggcgctgg tgacggggct
     2761 gttcatgggg gcgagttgtg cggccacggt cgtgggaatg cgaagggccg ctcgttttcg
     2821 ggcattatgg ctgatggatg cgtggcagct cggggtggcg atggctttgg cggtcggcgt
     2881 ggatgcgatg atgcaagcgg gggcggatct gacgcgcgca ggggtcatcg tggccgcttt
     2941 gctgatgggt gccgccaccg gagggcaatt cgccgtggcc tcgcgaatgg acggggtttt
     3001 tccgaccggt gtgggggcat cggtgtacgg cctcgacttg gcgggcgcgg cgcttgccgc
     3061 cttcgtttcc gtcacgtggc tgattcccac gttaggaata tcgggaacct tatgggccgt
     3121 ggcgggatgc aaagccgtga gcctcgtggc tttggtaacc cgatcccggg tcagcgccga
     3181 gtccctctcc tttcggatgc ccctgcccat gatgctgctc ggcggggtga tcctgcttgc
     3241 gattcaatcc acccatggcc cgtttcagcg cttcatcaac tccgatgggt atcggcttgt
     3301 cgctatcctc gttttggccg ccgcgatggc gtcagctttc gagcccatgc catggcgccg
     3361 ttggattcgc caagcggaac accgcagtcg cgtcttcgcc gaacggatcg gcgtgtctcc
     3421 cttgcgcctt ctcagcttgc tggccctatt gcccgtagca gctcaacccc tgggacgctg
     3481 catgtttgcg atcccctatc tattttgtca cgtatgtccg cgcccttgta tctttggggt
     3541 tctgcgtcga ttcgtcatcg cctcggcgct cgtgaccaac ttgggagact tacggttttg
     3601 ccagcgcgta tgtccgttgg gtctcgcaca agcgtcggtt ccctcgacga gttcgttgcg
     3661 caagctcgga aaagcctcct ggtggttgcg gatcccctcg ttggccttgg tcatctggtg
     3721 ggtgtttgga atcgatcctg gtccggtcca tgaaggggaa gggatcgtcc agcggctcta
     3781 tcagcatgcc gatgcggtca atccctgggt gttatggatc acgggcggct tgctgctgct
     3841 ttcgtttttc gtccgacgac ctttttgtga gtcgatttgc cccattggat ccacgagcga
     3901 gatattggta cgagcggacc ggctcgtgcg ccgtcgacgt tccccccaac caccgtgatg
     3961 cgccaggaat tggccttctg ttccgcagga attctgatcc tcgtgctata ccgccgccgt
     4021 gcgtctgatt cctcgaagca gcagccagtt caacaccaaa ttcgcggtcg aactcgatga
     4081 cggcgcgtgg gtggaagcgg tgttgtaccg gaaacatact ttatgcatct cttcgcaggt
     4141 gggctgcgcc gtgggatgcc cattttgcgc gtcaggaagt cgcggttgga tgcgcaacct
     4201 tcacgcggaa gaactgatcg cgcaggtgca gcaagcccaa tcgtgggccc cctcccttcg
     4261 ccgcgtcacc gtctcgggcg taggtgagcc tttgcacaac gcggcggcgg tggagacgct
     4321 gcttcattgg tgtcgccaac accacctggc gccaagcttg accacaacgg ggggaagcga
     4381 ggaaaagctg aaacatttca tggggttacc acataacggg ttaaccttgt cggtccatgc
     4441 gggcaccgaa tcgatgaggc gcaagctcgt tccccatggc ttgtcgctgt ctcgattgtt
     4501 cgacacggtg gagcaatgtg taccgcggtt gagtcgttcc cggcgacgtc gtttatctct
     4561 tgcgtatttg ctcatcggcg atggcaacga tgatgcggag cagactggtg ccttttcgga
     4621 gcgcgctcga agccttggct tgtctgtcta tctttatcgg ttcaatccgg tggcccacgg
     4681 caactttctt ccggccgcgg aaaagcgata cgaagaggtt gtcgaagcct ggcgagccat
     4741 gggtctggaa gtgcgttgca gttccctggc ccgcaatgaa cccaatgggg gatgtgggac
     4801 cctgatggcg caagcgggcc gcgttttcga cgatggctcc ggaaacgaga accagcttgg
     4861 atgagaccgg aggagtatcc gtatatgtat actcggatgt ctggcggcgt tggctcagcc
     4921 caccagcatt ttcttgcgta aatcgagggc cgttttctga tctcgcagct tctcgaccaa
     4981 cgtgagtgcg aagtcgagcg cggttcctgg cccacggctc gtgaccatgt tggcatcgac
     5041 cacgacccga tcggacagca cagtcgctcc ttccaagtag gtttcgaagc ccggatacgc
     5101 ggtcatcttg cgtcccagat gggctttcgt ggaggcgatg gccatcggtg cagcgcagat
     5161 cgccgccacg agtttgccct cttgcgccat tttctccacg agccattgca cgcgctcatc
     5221 gttcgccaag tttttcgctc cgggcattcc cccgggaaga acgatggcag ccagggactc
     5281 cggtcgaagg tcccgcaacg tatcggtggc ttgcacgcca atatcatgcg caccggtcac
     5341 gagcccaggg gccttcgctg ctacgatcac cttgatattc gcccgtcgaa gcacgtcgat
     5401 aatcgttacc gcctcgatct cctcgaaccc ttccatcaac ggaacagcca cggccaggtc
     5461 gtccatcact catctccttg tcacgccaag tcgcaatcaa tccctgtcga ttcgcccatg
     5521 atctccacca tgagcccaca ccgctgtcag aacatgaagc aaggataaat ggaaccgcaa
     5581 ccgccaacca cgaaggccgt ggccacggtg gcgcctggca aacggcccca ccgactataa
     5641 ctcgctgatt taattgaatt ttatctataa gcaacgagcc tcacgacgat ggtttcgacg
     5701 cgggggattg gtggcgaatc gccctggcga gccctggtca ttcgacggtg acagggattt
     5761 gcgcgacatg gtgaagctgc ttgggatccg tgagaaagcc ttgcacaacg atcgttccgg
     5821 tgccggcctc tttggctcga aacgtagctt ctcgtcgggt caacgaagct tgatcgaagt
     5881 aaacctcccc cagattggcg ggttcgactt tccaaatctc gcgctgaaga cggtcgaatt
     5941 ttttgggtac ggacaaggaa agggcgacgg tatccccgac tttggcttgc accttgccgg
     6001 gcgtgatctg accggtggga gtatcgtttt gacatcccag aatcagaacc gacgaaagga
     6061 gtgtgcagag cagcaactgg gctcgtctcg atagcatgat gatccgagtt gtatcacagg
     6121 cgagccagcg agagggcaat agaaggaggc gacgtggtcg agggcggggc gggagcaggg
     6181 aatgggttgt tgccgatggt tcagcaaacc aagggcaagg gatcagagtt ttcggacggt
     6241 tttgtgggcg gaccacattt tgctgagacc atcggcgagc ccagcgtgca attcggcgac
     6301 gagtttcgga tcccaggagg agggttcatc gcgtacgaag gtggttttgt cgacgcggtt
     6361 gtcgaaacgt ccgatgggca ggaagtagtc tttgccgccg gcatggatgg tgatggtgtt
     6421 gggcaccttg gaggtttcca tcaagtaggc catgtcgtcg caaccgtagt cattgatcat
     6481 cacggactcg ggagccgcgt ggaataggat cgtgttttcg gcgttgggca ccgcacgagc
     6541 gaggttcttt cgacgggact cctcgggctc gacccagaca tagagcacgg cagccttttc
     6601 gaggatttcc ggagcaagct gcgcgaggct gtattgatat ccatgggggg cttgcagagg
     6661 catggtggag ccttggggtc caccacgggc aaattcgatg acgatggtct tattttccag
     6721 cgagtttgga cgttttccaa acagctcatg aaccacccat tcgacctttt tttgcatccg
     6781 atcgccgagt cgtttgcgga gatcggcgct catcgactcg aacgccgcgg ggacgcctac
     6841 ttttgccctg gccgcatcga tgcgcgcaaa catcaccgcg gcgtcggcct tgggagaagg
     6901 caggtcgggg tttttcatca cccggtaatc ctcgttgacc aactgcaaca aagtgcccca
     6961 atcgtggccg tactgaaaac catcgtcggc acttttgaag aatcgacggg cttccccgag
     7021 ttcctcgaga gcatcatcga tctcacgcat caactcgaca tacgggtaat catcgagctg
     7081 gaccgtgtcg gcgagatgaa attgttcgat gcgtttcgcg cgatcgacat gcatcatgta
     7141 gcggcgaatt tccgacttac cggaagccgg aagagcgaga agaagaacgg tatcgacgaa
     7201 gttcgggctg gtcataacaa ttccttttca ggggcttgta aggtgtcgat ccccaatcga
     7261 atatcaacaa tacgagtatt tgagcagccg catcatcaca gaagtcagga cgacagcagg
     7321 agcgattcat ccgcttcgaa gaaaatgatt tctcggaatt cgggcccacg acgggtgcct
     7381 ggctacggac cgtgagcgat cacgcttgct gatgacgccg attgtctgtt cctccgcggt
     7441 atgctcaaac cctccgttgg ggagttccac ccacttggaa aggtgtgcgc gtatcatagt
     7501 ccgcgcttcg gcagggtcaa cccagggctg cacgtagacc ttgagtttgc gcccacggtt
     7561 gattaggcag cgattcatca cgcaacacaa ctccttgcaa gacggccaat gaacctattt
     7621 cgcgcaggaa aaaattggtg gcacgtcggc ggccgagcca tcgtttacgg atccatcagt
     7681 gtcaccgccg ggaccatcac caaacgccac gacatcggac agcgctgcat gcgggcctgg
     7741 tgtcaaggat ccctggacgg attgggaatc caacactcga ttcgaaatgg atcgctactc
     7801 gatagcgcca cgccatgcgt gttcgttgcc aatcacctca gccaactcga tatccttctc
     7861 atcggctcct atctcaaaca ggattatcgt tggctcgcca aagcctcgct attccacatc
     7921 cccttcatcg gatggcatct atccgtatcc ggtcatgtcc ccgtctaccg cggagagcat
     7981 aaagacaaaa acgaaatgct cgcgcaacgc atccacgacg tcgtggtaca aggcgcaagc
     8041 ttgttgtttt tccccgaagg cacccgaagc cgcgacggtc gcctgcactc gtttcgcatc
     8101 ggagcgttcc gcaccgccgt gcgtgaaaac ttgcccatcg tccccctcgt cgttcgcgga
     8161 acccacgaac tgttcgtccc tggctccaaa gacctcgccg tacaagccga gcgaaaatgc
     8221 tccctcacgg tgcttcccgc aatacatgcc ccctccgatg gcgacgaaaa gtcacgagcc
     8281 gccgaattat gtgagcgtac ccacgccgcc ttcgtggccg aattgaccgc cccatcgagc
     8341 gaaactcaat aaaattcaac gctttatcgc ttccgtgacc gccgctagca attgatcgag
     8401 cgatacctgc accggatcgt cctgcgttcg cacacggagt tccggcttcg acggcgcctc
     8461 gtagggatcc gatacccccg taaactgcgg gatttgtccg cgatccgccc gacgatataa
     8521 ccctttggga tctctgcggc gacactcctc cacagaacaa tcgacatgga cttcgaaaaa
     8581 tcggtgtggc ggtagacgct cctgcaccgc tcgtcgatcc ctttcataag gagacacgag
     8641 agccacgatg gcgatgattc cttgttcagc gatgattttt gcgatttccc ccgcccgacg
     8701 tacgttttcc tgacggtctt tcgcggaaaa accaaggtcg gaacaaagcc cctggcgcaa
     8761 tacgtcgccg tcgatgatgc atgcagcctg acccgctttt tcaaggctat cgcgcaacgc
     8821 caacgccaag gtggacttgc ccgccgcggg aagcccagtc aaccatacga ccaacccatg
     8881 cgaagtaccg gtcataccca cgactcgtac cacgaacgcg ccaacaagaa aaagcatcta
     8941 gttgcgcgaa gttggctagt ttttttcacc atgagacaat caccccatgc gtgaccgcga
     9001 cccacgacgc ctagaacaaa acctgctccg tgacccacga cgggacccca ccgtcccacc
     9061 ccccacggca gagcaggacg ccccggccac gcctatcatt gtcgaggccg ccgcagaatc
     9121 acccccaccc ccaaaagcag aaccgccttc cagccgtcgg ggcaacctcg atgctaccga
     9181 ccccggagca gataggcctt ccagcccaca accggaaggc gcttcgaaaa tcacttcccc
     9241 cgacccccgt attgccgaac tcgaacccct tctggcatcg ggccagtggg aaggcatcgt
     9301 caagtacttc ggctcccgag gaaaacgccg gaaaactgcc acccaacctc gggctcattt
     9361 acgccattgc acgcaaagaa ttagaaacgg ataaagacaa ggaaactaag aaaataaagg
     9421 aacatgaaga gcctcatcag gtggccattc gttgtatagc cggactgtac gggatatcgc
     9481 ccgatagccc catcgcgatc gtgttaggaa aacggatcat ccgcgccaat ccggtatcct
     9541 ggcaaaagaa aaaggcacct ccagctccga tctcatttct tatcgtcgcc ctgggcttgt
     9601 tgctgggggc aggaatcggc tggctgctca gcttcggcag cgtgaccctc aaattgccta
     9661 atctactgtt tttacactag tttttttctg attcccgacg gccttcgaca ggagcgacaa
     9721 ccctcggaaa agcggtcgtg acacgccccg gcgatcacgc tccaacaacc taaaaattct
     9781 tataaataca atatgttact acagttttcc cagacgacgg cgtcgggcgt tcagggccga
     9841 agggcccagg aggttgcatg ggcgccacgg cgtacgcccg tttcgacaac cctttcgcat
     9901 tcgccctgcg gctccgccac ctgcctcaaa attctctcaa aatcatggtc ttgcatcggt
     9961 cccgtacaca aaagggtgct caccacttcg tcgaaaatcc ggtccttcga ggatcctggg
    10021 tgttttttca aaaacgcgat gatgatctcg cggactcgct cgtcgaaagc gtcttgtccc
    10081 accacagtgg cggcgggaaa cacgcgggca cgaggtgatt tgcaaagatt cagcaccaga
    10141 tctcgcttgt tgaccttgtc gaccgtgtat tgattgtacg ttctgctttt cgtatcgatg
    10201 ctcgtcgcgc ttcccaccga ttgcgcatcg aaacctgcgt tttcaatcgc ctgctgcaaa
    10261 gcgctccaac tgcctttcga cccatcgtgg tagcaaacgg acatccatcg gtttggtttc
    10321 aggacccgat gacattccgc aagcgcccgg ctcattcgct ccgtccacgt ttccaaggac
    10381 aaaccgcgtg tccgattgac gatgatttca tgacgatgcc aatccgtggg caggcccagc
    10441 cacgcttccc aaagaaaatt cagttccccg tattgcacca tgcccccata aggaggatcg
    10501 gtgaaaatat agtcgatgct cgcatcgggt atcgcgctca gatccgtgca ggattgcgtc
    10561 gacagacaaa catcgcctcg caacccaaga cgttgcaact gttggcgcgc caccagcgag
    10621 cggccgaata cccttcgcac ggtttccaaa acattgcgct gcttggtcat ggggggcaga
    10681 gcccaatgac cagggatata gccacctccc ccctcgagat gctgtgcttt tcggctgacc
    10741 gccaacatgg cgctcgtcag cacggcttga agatccaggt caccgtccgc cgcctcccac
    10801 aacgctgcca aggcccataa atttcgatgg gtaaataggt cccgaatgca cctaaagttg
    10861 cggcttggac gccattcgtc cccccacggc ccatcgtcga tcctattcat catcggcaca
    10921 tcgggaatcg gatgaggaat gggcaatcgc tccaggcaag cgatacccgc aagatcgatg
    10981 cggtcgaacg cttccctttc ctccctggtc ccgacgatac cgcgggtgct tcgcttcgga
    11041 tggcagccct ccaaacaaga aaaactcacg ccaaccggca ccaatccaag ccggtcgagc
    11101 gacgagtgga ttcgcacccg ttttccttgg cgaagacacg aggggcaaca agacggccct
    11161 tcttgttgta atgcctcgaa aagacttgtg atttctttgc atcggggaca ggcatacgtt
    11221 tgggaataca ccagatggtg cagcatcgct tttcccccac aacgatggca gcgcgtcgca
    11281 tacagccatt gcaactcatc ggccgcgcgc tggaccatgg cctcgcaacg agccagtaag
    11341 tcattgatat caatagggaa aagatatgcc cgggcaatga aagtagcagc cggggaggcg
    11401 tcgatcccga tcgcatgacg atgttcttgc aaggccgcga gcgccgtgct tcccgatccc
    11461 gcaaacggat cgagcacgac atcccctggg gcggtaaagt gagccacata cgcgcgaatg
    11521 gcatcatggg gctttttgcc aaggtgatac ccatgcaaag catagatggc agactgcttg
    11581 gtcgtctgaa tctgagccga aaatgggggc cgatgataat cgtccgtcga tgggtcgaaa
    11641 ggatgaaccc gctgccgcac ccatgtttcc aggtgcgggt tggccccgtg caacgagaag
    11701 gaacaagtgt gttgggagac gtggtcgctt tcgttcatga cagaggcgta gaaaggcagt
    11761 gcatgcgatg ctttcgaaag gagcgatcga ttctcgactt ttcggggctc ctgtcccgtt
    11821 gacaacgtct tcagactccg atgggatagg agcatgccac cgaggatcgc atgtgtctat
    11881 ttcctctttg tcctttgcct tggcgcgtgg ggatgcagtt ccgaggaccc cccgagcgac
    11941 gaggacacgg gtcccatcga gaagccctgt gaaggctatg cttccgcgcg gtgtgagacg
    12001 atgaatcgct gtgcgcctgc cctatttgcc atcgagcacg gagatctcga cgcctgtcgt
    12061 tccgagcttc gtgcatggtg tgtgcatgtg gggcatttac caggggttcg cgtcggcgcc
    12121 aaagcgatcg gcgcgtgtgc ttcgacgttt cggtccctat cgtgcgccgc ttggcaagcg
    12181 cgtcgtgacg atccattttg cgatgcgccc ggcggatctc tttccgtcga cgagccctgt
    12241 atttccggtt accaatgcgc ctccaaagca tgctacctgg acgcccaggg ggtttgtggc
    12301 gtgtgccaac ccacggcagg ggtgggagat ccttgtcgcc aatcgatgga atgccccgca
    12361 acgagcgctt gcgtaggcta tcactgcctg aaaactatgg ctctggggga agaatgcggc
    12421 tcgggtcgcc cctgcgccgg acaactgctt tgcctttcag gaacttgcca acaaagcccc
    12481 gccttgcatg acccttgtat cgaaaatacc tgcaacaacc gattggccac cgaatgcgtc
    12541 cagggggtct gcgaacccct cgaaacagca gctacagggc aggcttgtgg gctcatcaac
    12601 ggaaaaaccg tgtattgcgg cagagggacc tgttcgagcc agggggtctg cgtggcgccc
    12661 gtcccggaag gtcgcccttg ccaggactcc tccggcccca cctgtctgca gcccgccgta
    12721 tgtgtcgacg gctggtgcaa aatcctccag ccatcgatgt gtcaataaat accggattct
    12781 aaagaatata cgtattacca ttccattcgt tacgagcgga tttcctcgtg ggatggctcg
    12841 cccgagaaac gccctggtcg tatcgggacc agggctaatt atgtgtgttt ttgcttgctt
    12901 gcggtcggaa agagccgttg tcgggttttt cgaaccgtgg attggactgg ggtcaggatt
    12961 cgaaagcggc gggagttggt cgggtagtcg gagcgtcgtg acaggatagg agagcggcct
    13021 gcggcagagg ttccgttctt ggcaggaagg gtcgatcgag aacccgaccg ggtcgatcat
    13081 tccctacgtt ttgagtattt tctgggagtt ttttcccctg cggatcggcc ccacccgggg
    13141 ccagggccga tgccctggtc gaatatccct tcgcacgatt tccaccggaa tccctcgggg
    13201 acatcgtctt gtttttccac gccgctagat cacaacccgg cgaggggcta tggtggcctc
    13261 gaccagaacg tcgatgaaca aacccaaagc agcatctcgc taccatgccc tggatggcgc
    13321 ccgagccttc gccatgctgc tcggtgtgct gttgcacgcg tcgatgccct acttattcgt
    13381 cttgtttccg tgggccgtgg tggatggctc ccgctccgtg agtctgacac tgctcatcta
    13441 caccattcat tcgtttcgta tgcccgcctt ctttttgatc gccggttttt tcgcacgtct
    13501 ggtgcttcaa cgagatggcg tgggagcatt cgtcaaacac cggcttcgaa ggatattggg
    13561 acctctggtg gtgggttgga tcgtgctcta cccgctcgtg cgctttttat gggtgtggag
    13621 cggtgtgcgc atggtgcaag gggccgatct ctatcaggcc gtggtggcag cttttacccc
    13681 cccctttctg ggtcggggtc tcggcctgat ccatctatgg tttttatatt atttattgct
    13741 gctttacggg tttgcggtcg gcatccgatg ggtgggactc aaggtggaca ggcagggcaa
    13801 acagcagcaa cgcctcgacg ccctttttct tcgtctgctg cactctcgcc ttggcatctt
    13861 cggattggcg attcccacgg cttgcgcctt gctccccatg cgaggatggg gcgtggattt
    13921 tgccgatcga agtctgtggc cccgagctgc ccatctatta ttctatggct tctttttcct
    13981 catgggctgg tggctgcacc gccatcccac cttgctcgca accctacgaa atcgctgggg
    14041 catgcatgtc gccatctcct cggttttgct atttcccatc actgggttgc tgtattggat
    14101 atattcgctc cattacgacg ccggcttcgc ttttcgaatc gtctattttt tgctttacgc
    14161 cacgatatcc tggggattcg tgctgggatg gctcggcgcc ttcgaacgct tttttcaccg
    14221 tcccaacgcc gtgtggcgct atctcgccga cgcctcctat tggatttatc tcgtccactt
    14281 gccgctggtc atcgctcttg ccatcttgct tcgaagttgg ccgataggct ggtatatcaa
    14341 actgcccctt gtgcttgcct cgagcttgcc cctgctgctc ggaagctacc atattttcgt
    14401 tcgctatacc gggatcggta tcgtcttgca cggacccagg attaaaacca ataaaatcaa
    14461 ttagttataa caagtccgca gatcatcgga tgcaccttgc gtggcaaagg cacgtaaaca
    14521 gggaagcccc cggcacgtcg gcatctttgg atacctggag cgtcgtctcc cccacgattg
    14581 cgtttgccgt gacggtgacc gtgaccacgg ccaaatgagg ggacggatca ggctcagggg
    14641 acgttgacgg tcacggaaag tttggcttgc tcgatgaggc aacgttcttc ggtgcagacg
    14701 gaaaaatgga gggtgccgga gacggtagcg gggcccgcct gggcgggcac gaaggggacg
    14761 gaaagggtgg aacgtttggg cgtgacttgc atgcctcgta cggtgtcctg ggggtaggag
    14821 atgccggcgc cgggcgggtc gagtttgaat ttgtagggat atttatcgtt acatttgtag
    14881 gggtctttgg cgacgagaac gacggtgagc gcgccttgtt ttccagcttc gtagggaccg
    14941 gcggattgga gccaggcgga ataggaggcc gcggaggcgt cggcaccttt tttgggctct
    15001 tcggccgcgg aggctgtcga tttcggctcg gggcgagtat cttgcccctg ggacggcggg
    15061 gtggtcgtcg tgggcgcacg gaccgcagtg gcggaagcga cgggttgttc cggttccttg
    15121 gcggaattcg ccggagtgtc cgcaacagag ggaggtggtg cggtctcgat agccactatt
    15181 ttttctggcc ccggttcgac gggagcggtt ttttggtcac aaccgatggc gagaaaactc
    15241 gaaaaaaaga ataccgacag ggaaccaaag gcaatgcggc tctttcggag ggaagggaca
    15301 tggctcatga tgtgcaagta gcgcgaaaca gcgtgggtgt cgatgggttg tgtgacgtga
    15361 aaacaactct tgtgccgacg cggcggagtt gtcgagcgca ttacacgttt cttgccccat
    15421 gttccggaca t
//
LOCUS       DAOSOR010000056        15427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_3853_length_15427_cov_4.463115, whole genome shotgun sequence.
ACCESSION   DAOSOR010000056 DAOSOR010000000
VERSION     DAOSOR010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 15427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15427
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3853_length_15427_cov_4.463115"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PK710_04800"
     CDS             <1..392
                     /locus_tag="PK710_04800"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOT09065.1"
                     /translation="FQAHPEWFGEEVAGHLTKGAAIKAVRYLQAQKERQSIVRAIDIL
                     FTFSDLLILPTTPIVSTPAQEPGTQLPFLALTVPFSLGGYPAVSVPMGEVDGLPVGLQ
                     IVARRGDDYLALAAAIAFEAAFQAARP"
     gene            454..1776
                     /locus_tag="PK710_04805"
     CDS             454..1776
                     /locus_tag="PK710_04805"
                     /inference="COORDINATES: protein motif:HMM:NF037048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOT09066.1"
                     /translation="MARVDRLHIGIFGRINAGKSTLMNVLTQQQTSIVDPTPGTTADI
                     KAAVMEIHSLGPVKILDTAGLDEGNVLGAKKRDKTLSALEQVDIAVLVVDPVQTLQSG
                     DLTIEEQVANTSRRLGQSLVMLCNLHADHESRLQACGATLEQALDHCKSALPDRAGTP
                     FLVTDLTQRGALEAVIELLGKGRPSPQKPVQLLPFVGGSAPVLLHIPLDEESPSHRLL
                     RPQEMAIEALLRLRVPVAVTRVDLKLARTGNALMQTREKDKFLRFFNMLGGEGGVQLV
                     LTDSQAIDLMHAWLPALVPLTTFSVMMIHQSCGGDLALFAEGTRVLGTLRPGDRVLIA
                     EACNHDRMAEDIGTVQIPKKLEQRIPGIHIDHAFGREFPDPQNLRAYRLVIHCGGCMI
                     SKQQASARISRLVEATVPVSNYGLTLAWLESATALERVLRPWLPNSSK"
     gene            1894..6510
                     /locus_tag="PK710_04810"
     CDS             1894..6510
                     /locus_tag="PK710_04810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT09067.1"
                     /translation="MPQTFPDTLEAWTQDPCPPRALDVLTVAEQTIGSAAQQQEIWHH
                     FLDITRLSAFLSALPDREHRNRWAEICLQALDSTHFTLETLLDQRTRRHPDRILFQER
                     DASQSSGWSYAQVRRRARRIAAGLLALTDTPRVAIFADNGVDSACTDLACLAYDILDT
                     PLNVHFDTATLTNITERLAINIVVTDSDSHYARWLEVRQATKLPFRILRTDNASSLEP
                     QDTSLGAIYAPLGEEEVEDRLSHRKRLGWKEAATVMFTSGSTGEAKGLVFSQRNLVSK
                     RFARAAALPAVDEQEVLLCYLPLFHTFGRYLEMLGMLFWGGTYCFAGNPSAETLLTLL
                     QKVRPTGLISIPLRWTQIHDHCLSSMNKSPGVPARTTFETETGGRLRWGLSAAGRLDP
                     KVFRFFHKMGVELCSGFGMTEATGGITMTPPGEYRDGSVGIPLPLMRTRINDIGELHI
                     GGPYVAHYLGDPPEGEDPWIATGDLFMPQADGHLEIVDRIKDIYKNSRGQTIAPGRVE
                     QKFADVPGIKRVFLVGDGRDYNALLIVPDLSDPIMNNFEPSPIDDPDAPIRTYYRQIV
                     SAANKDLAPYERVVNFTLVERDFSADQGELTAKGTYRRKTIQENFAPAIRELYRKRFV
                     ELRVRQWSIRIPRWFFRDLTELETDIVADDLGLCDEPSGRRLDMQEGSEPGSVRIGDL
                     EYAFDTNIIDLGQLARQPFLWVSNAALVAFAPCKDGWDVTLDGVSPRVLLPWKAPVLT
                     EKEAGLERVPPSLRLLEVHRVSLDALYGRGQTALEAAKELSRMLESIDPRTGELVRRR
                     LEALARHPDLEVRCHAYQSLLLSLRVPTYDSMLRSFVQAGLKFLNEETIEIIAQEKPE
                     RRRLEAFRQRLHGYRSQLPWPAPDDTRSVFIDVFKLLASLARYHPEFYGAVREELVAW
                     IMHEPAPKLAAAAERELHALAARFEQSLTGSCADPASWQGRIVFQDGLHAEEAAQLQR
                     IIVGTSFLKQAIMLSTDEETCDIERIVPDGIWVSRISTLHQHASYRVSINTDTGKHYD
                     LQIVIPKDISHEHVLRTIYWIIGIRGYPFGQPVLPKFGCWRPELGALALAYVSDLTVW
                     ERIRAFASFRIPGTEYPPPEAWRKLFVRAMAAFFAGWRASGRRIVPGAINPSNVAVPG
                     PDFQEGTQILSLTDWKPYDGPKTLVAPLVRNFYVQTVSHYPWCAKHLDPDWILQACIE
                     AIGEEEGTNFLRELDIVMGSEKIPGAGIPWHDRIEPFLSRLQTHPFVPLAVSCAEQRY
                     HKWQRENPDATSTAKEQCVLAVQALYGFERFPAWVRFHLYAQTYFATFGKEVQDTFQR
                     LIQEMARNPGRDATSLIEVSDLQAWLESPEDRAVFNRAVFPKTQPTQPLEFLAVRHGE
                     QQKVILQTKVFDVDSVPYCVREPLEPSEVGKLYRHYVTAGFHKTISTQDHYLVLLNET
                     EQIVGGLSYRRMDDDVVHLDGIVVSGYLRGRGLNGAMLEDFCARMVNEGTKVIRTHFF
                     VRHFYMQRGFRIDRRYGGLVRFLTNESTPEHPPSSMVLPHLLGD"
     gene            6691..7098
                     /locus_tag="PK710_04815"
     CDS             6691..7098
                     /locus_tag="PK710_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT09068.1"
                     /translation="MAETTILLVDDDDDLLEVNRLALEAAGFGVLIAHHSAEALAIVQ
                     QHTIHGIVLDVMMQTRDEGFHLARVLRKTPQTRNVPILMLTGVNAESARQGHPLRLSD
                     DDRDEMWLPVDRFLDKPVSPERLIAMLRELLGE"
     gene            7100..7834
                     /locus_tag="PK710_04820"
     CDS             7100..7834
                     /locus_tag="PK710_04820"
                     /inference="COORDINATES: protein motif:HMM:NF003996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOT09069.1"
                     /translation="MSKKPGPHRPSAHGQRVQESSVPLPRPLLVRLAAYHQIATQHGE
                     EGNTTIRSGHMAALLNIDPTLVRKDMAALGITGRPKVGYPIGTLLPCLDRILGLTERK
                     SAILIGCGDLGSALARYGGFSRYGLQISALFDIDQNKMGKTIAGLTVQPMEKCRRYIE
                     IFSIQIAILTAPSPQAQDIADWLVARDIKAIWNFAPVHLKAPPNVVVRNENLALGLAQ
                     LLHQIKRSKTTAPAAEHDALRRRESA"
     gene            7836..9917
                     /locus_tag="PK710_04825"
     CDS             7836..9917
                     /locus_tag="PK710_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOT09070.1"
                     /translation="MESGPIIDTLQDLCKRCYSCVRRCPAKAIRVQNGQAEVIEQRCI
                     GCGRCVHACSQGAKRVASQLTHVEQLCRRGQTVLMLAPSFPAAFETWSAHQVIAAARQ
                     AGFAGVYDVAFGADLVARHHRQRYEREPGRPIITSACPAACAFIQKYAVELVPFLAPV
                     LSPMAAMGKALKTIVQPGCYTVFVGPCSAKIREIRDLDVAPWVDAVLTFPELAALFER
                     RSIDPRVLPEQDFDPPFAGLGGIFPIPGGLARAAELPSDLLENHVFQLSGIDEFVDGI
                     ERMRERATCGVLLEQETRLFDVLFCKGCVAGPLMPSRGSLLVRKEKVVAFMRSYTGRP
                     PSLDSATMRSLLEMDISRSFDPDNQRRQEPTEEQIREILAQTGKYNPQDELNCRACGY
                     RSCREKAVAVYHGVAEVDMCLPFLVEKLVATIGQLNRSHEELTEAHAQVVRVEKLASM
                     GQLAAGIAHEVNNPLGSILLYSHLLRESLEGSIPPNIEQVRNDIDMILQEATRCRGIV
                     GGLLDFARQSKVSRAPVLLSDLIDDTIRVSRTFASSQDLRLENRSPAGLPLANVDRDQ
                     LLQVLTNMVRNACEVMPDGGTIWLRADWISERNEFRIIVHDDGPGIPEQHMNKLFTPF
                     FTTKGVGKGTGLGLAISYGIVKMHRGSITAKKNLDGPGATFEITLPAVHSQANGNGNG
                     LLGADTAGQAP"
     gene            9914..11482
                     /locus_tag="PK710_04830"
     CDS             9914..11482
                     /locus_tag="PK710_04830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT09071.1"
                     /translation="MTDLLSTPAESPAAEPERPTILVVDDEIGFREGLRRILGHRGYR
                     VETASDGQEALDLASQRAFGLALVDLKMPGIDGFEVIERLQTISPQTVCIVVSAFATV
                     ESAVQSTKKGAFDFVVKPFTPDDLMVVLLRAEERWRLMTEAERLRQERDSHLLEIATE
                     KGRLRTIIDSMADGIMVVNIDTNIVLDNPEARRLLGRVSMSRLEGQVADYFEDEALVQ
                     EIRAMLTRGDALPVTRETKLSTPGPEQPDRYLRATLAPITEGPPDTPPLGVVVILTDV
                     TQDKAFERMKNLFVSMVAHELKAPIGAVESYLELIRMGALDGDPARIKAVAERCLLRT
                     RSLLDLIRDLTEMTRREGGFLERRLQRIDVSAFVAEIVEFQSAQAASRNITLHFEAPK
                     TPPILVADRGDIERIITNLLSNAIKYNLPNGRVDVRLGTSSGTIVLEVTDTGVGMSPE
                     DVARLGEEFFRAKNAHTRTVTGTGLGIALVKRVVDSYHGALEVDSALGKGSTFRVILP
                     AAPSASITTKGGSV"
     gene            11479..12939
                     /gene="hydG"
                     /locus_tag="PK710_04835"
     CDS             11479..12939
                     /gene="hydG"
                     /locus_tag="PK710_04835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOT09072.1"
                     /translation="MNLPDSKQQSNKAEPVQAQPMPTTAMAPDKVIVDEARIAHTLSS
                     CGGSDPVHIREIIAKAKELRGLDLEDTAALLTCDDPALLEEIFAAASYVKDAIYGRRV
                     VLFAPLYYSSTCVNNCLYCGFRRDAKRSRRTLSLEEVAREVGALEREGHRRLLVIGGE
                     TPGPKAIGRLMDVIRTVYETRDGGEIRRVNVETAPMTIEEFRQLAQARIGTYVVFQET
                     YHRATYKAMHPDGPKADYDWRIEVMDRAMRAGIEDVGIGVLYGLYDYRFDTLALLEHA
                     RHLETVHGTGPHTISVPRVEPTPDAPLSLAPPAALTDLDLCKVVAILRLSVPYTGIIL
                     STREPPQLRDKLLDLGVSQMSAGSCTEPGGYAGEHDSASQFPVADTRSLDDVIRSIAH
                     HGYLPSFCTGCYRKGRTGADFMELAKPGLIRMHCLPNALFTYLEYLMDYASEATRQVG
                     LDLIETQVEHEVPPARRNTVRKMIERIRAGERDLYL"
     gene            13142..14962
                     /locus_tag="PK710_04840"
     CDS             13142..14962
                     /locus_tag="PK710_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09073.1"
                     /translation="MRTGHLLMLAALSSIPIGCIGDSFSSEEPGPISSPDASSDGSDA
                     SSDGSDASSDGSDASSDAVTEREPDVPIDSMPEDGSDETDQGGNEAGKTATGAACNED
                     DECETNMCQDGVCCESVCDPCFSCGQPGHEGQCFAVPDGENHNEACGPAGSPCMGVCD
                     GSGHCTYPVGANCDDPHCDSDPWKQVLFQCNAEGLCEEQIVSCSPFLCKQAECLSKCD
                     DSDDCEDDAYCASSECVPKLVFGQTCTQNVECLSSHCADGVCCNDSCSGVCETCAFAG
                     KEGQCVAVPLDMDPDDECQGDGSTCAGTCSGQRSCVFPVHGEPCGESQCDANLNAAHE
                     LICNGEGKCVENVQNCGLYDCHKDIGSCLYSCSQDQDCIDDAFCNNDVCVAKKSNASP
                     CENSIECLSGYCEPTSHGKQCCNTACPHPLNCSTGECLCDTVVCSPGVNCVPWYRDRD
                     GDTFGNPNEMKLGCENAPPNIPGEKYVRNMDDCFDLNHLARPGQTAFFPEHRGDGSFD
                     YDCDGKETKQFDDTNDPVCKDCKTYSKCLYCGIFSYNTYGYRCKTPTSSDNTCGPLVT
                     QSYKQLRPCGQSGTLYRCNNDDKLCDTAEVPTHDVVQQCR"
     gene            15040..>15427
                     /locus_tag="PK710_04845"
     CDS             15040..>15427
                     /locus_tag="PK710_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09074.1"
                     /translation="MAHAQTASEVEQAKRLFAAAEEDQANLRWAEALEKLEQVVMIKD
                     TAGVRFHIAVCQDRLGLPARSLENFERAQQLALSSGSDDVLELVGPEIERLRQRVATL
                     TIEVSSRVVDVRIDRKRKLPATTLLYA"
BASE COUNT         3276 a         4412 c         4357 g         3382 t
ORIGIN      
        1 agttccaggc gcatcctgaa tggtttggag aggaagtggc cggtcacctc accaaagggg
       61 cggcgatcaa ggccgtgcgc tacttgcaag cgcaaaagga acgccaatcg atcgttcgcg
      121 ctatcgatat cctctttacc ttctccgacc tgctcattct tcccacgacc cccatcgtat
      181 ctaccccggc acaagagcca ggaacgcaac tgcctttctt ggcgctgacc gtgccgttca
      241 gtctcggtgg ttatccagcg gtttccgtac ccatgggcga ggtcgacggg cttcccgtgg
      301 ggctacagat cgtggctcgc cgcggcgatg actacctggc ccttgccgcg gccatcgctt
      361 tcgaagctgc cttccaagct gctcgcccat aaccaacaca cggacccagg ggaatgcgct
      421 tgcgacggtt aagcgctcgg gtatggtccg cccatggctc gcgtcgaccg gcttcatatc
      481 ggtatttttg gaagaatcaa cgctggcaag agcaccctca tgaacgtgct cacgcagcag
      541 cagacctcga ttgtggaccc aacccccggc accactgccg atatcaaagc cgcggtgatg
      601 gaaattcatt ccctcggccc ggtcaaaatc ctcgataccg ccggacttga cgaaggcaac
      661 gtgcttgggg ccaaaaaacg cgacaaaacc ttgtctgccc tcgaacaagt cgatatcgcc
      721 gtgctcgtcg tagaccccgt acaaaccttg caatcggggg acttgaccat cgaagagcaa
      781 gtcgccaaca ccagccgtcg actcggccaa agcctcgtga tgctctgcaa tctgcacgcg
      841 gaccacgaga gtcgattgca ggcatgcgga gcgaccctcg aacaagccct ggaccattgc
      901 aaaagtgcgt tgcccgatcg cgccggaacg ccctttttgg tgacggactt gacgcaacga
      961 ggcgcgctcg aagcggtgat agaacttttg ggaaaagggc gtccgagccc tcaaaagccc
     1021 gtgcagctac taccttttgt cgggggttcg gctcccgttc tgcttcatat tccgttggat
     1081 gaggagtcac cgagtcatcg gttgcttcga ccgcaagaga tggccatcga agcgctgctg
     1141 cgtctgcgtg tcccggtggc ggtgacccgt gttgatctga aactcgcacg cacgggaaac
     1201 gccttgatgc aaactcgaga aaaagacaaa tttcttagat ttttcaatat gttaggtgga
     1261 gaagggggag tccagctcgt gctgaccgac tcgcaggcca tcgacctgat gcatgcgtgg
     1321 ctaccggcgc tggtaccttt gacgacgttt tcggtcatga tgattcacca gagttgcggt
     1381 ggagatcttg cgctttttgc cgaagggaca cgggtgttgg gtacgctacg gcctggggat
     1441 cgggtgctca ttgcggaggc ctgcaatcac gaccgcatgg cagaggatat cggcacggta
     1501 cagatcccga aaaagcttga acagcgcatt ccgggaattc acatcgacca tgcattcgga
     1561 agggaattcc ccgatccgca aaacctgcgt gcctatcggc tcgtcattca ttgcggcggt
     1621 tgcatgatta gcaaacagca agcgtcagcc cggatttccc gtcttgtgga agcgacagtt
     1681 ccggtatcca attatggatt gaccctggcc tggctcgaaa gcgcgacagc cctcgagcgc
     1741 gtgcttcgtc cgtggctccc caactcctcg aaataacagg gaaaacctgg catcccggcc
     1801 aatgcaccgc ccttcgcgtg cttttcgcgc ccccactcag cgcctatccg atatcccatt
     1861 gactttcctt tcaccatggc tttcccttga ttcatgccgc aaacctttcc ggatacgctg
     1921 gaagcctgga cccaggaccc ctgcccccca cgagccctcg acgtattgac cgtcgcggaa
     1981 caaacgatag gaagtgccgc ccagcagcaa gaaatctggc accactttct cgatatcaca
     2041 cgtctttcgg catttctttc cgcgctgccg gaccgggagc atcgcaaccg atgggcagaa
     2101 atatgcttgc aagctctcga ttctacccat ttcacgttgg aaaccctgct cgaccaacgc
     2161 acgaggcgcc atccggaccg gattctcttc caagaaaggg atgccagcca atcgtccggt
     2221 tggtcttacg cgcaagtacg tcgccgagcc cgtcgaatcg cagccggact gctcgcgctc
     2281 acggacaccc cccgcgtcgc cattttcgcc gataatggtg tggattctgc ctgtacggac
     2341 cttgcctgtc tcgcgtacga tatcctcgat acgcccctga acgtccactt cgatacggca
     2401 acacttacga atatcacgga acggctcgcc atcaatatcg tggtgacgga ctcggacagc
     2461 cattatgccc gttggctcga agtccgtcag gccacgaagc tgccatttcg gattctgcgc
     2521 acggacaatg cttcgtctct cgaaccgcag gatacatccc ttggtgcgat ttatgccccc
     2581 ctgggggaag aagaggtcga ggacaggttg agccatcgca agcgtttagg gtggaaagag
     2641 gcggcgacgg tgatgtttac gtcgggcagc acgggcgagg ccaagggcct tgtgttttcc
     2701 cagcgcaact tggtttccaa acgttttgca cgggccgccg ccttgcccgc ggtcgatgaa
     2761 caggaagtcc tattatgtta tcttccgctc tttcacacgt ttggccgcta cctcgagatg
     2821 ctgggcatgc tgttttgggg cggtacgtat tgttttgcgg gaaatccttc cgcggaaact
     2881 ctgctgacac tgctgcaaaa agttcgaccc acgggattga tatcgattcc ccttcgttgg
     2941 acccaaatcc atgaccattg cctatcgagc atgaacaaat ctccgggggt tcccgcgcgt
     3001 acgaccttcg aaaccgagac gggggggcgg cttcggtggg ggctgtcggc cgcggggcgt
     3061 ctcgatccca aggtattccg tttctttcat aaaatggggg tcgagctatg ctcggggttt
     3121 ggaatgacgg aagccacggg ggggatcacg atgacgcctc ctggcgagta tcgcgatggt
     3181 tccgtaggga ttcccctgcc tctgatgcgg actcgcatca acgatatcgg tgaattgcat
     3241 attggcggtc cgtatgtggc tcattacttg ggagatccgc cggagggcga agacccatgg
     3301 attgcgacgg gggatttgtt catgccgcag gccgacgggc acttggagat cgtggatcgg
     3361 atcaaggata tttataaaaa cagccgaggg cagacgatcg cgccgggaag ggtggagcag
     3421 aaattcgcgg atgtacctgg aatcaaacga gtttttttgg ttggggacgg ccgggattac
     3481 aacgccttgc tcatcgttcc ggacctttcg gaccccatca tgaacaactt cgaaccgtcg
     3541 ccgatcgacg atcccgacgc tccgatacgc acttattatc gtcaaatcgt gagcgcggcc
     3601 aacaaggatc tggctcctta cgagcgtgtg gtcaacttca ccctggtgga acgggacttc
     3661 tccgcggacc agggagaact caccgccaaa ggaacgtatc gtcgtaaaac catccaggag
     3721 aacttcgctc ccgcgattcg cgaattatat cgtaaacgtt tcgtggaatt gcgtgtacgg
     3781 caatggtcca ttcgcattcc acgctggttt ttccgtgact tgaccgaatt ggagacggat
     3841 atcgtggcgg acgatctggg gctgtgcgac gagccttcgg gacggcgcct ggacatgcaa
     3901 gaaggctcgg aaccgggcag tgtgcgcatc ggagatctgg aatatgcctt tgatacgaat
     3961 atcatcgacc tggggcaatt ggctcgtcag ccctttttgt gggtttccaa tgctgccttg
     4021 gtcgcttttg ccccgtgcaa ggatgggtgg gacgtcactc tcgacggggt ttcgccgcgg
     4081 gtgttgttgc cctggaaagc ccctgtcctc acggaaaaag aagcaggatt ggaacgcgtg
     4141 cctccctcgt tgcgcctgct cgaggtccat cgtgtaagtc tcgatgccct ctatggtcga
     4201 ggacagacag ctcttgaagc ggccaaagaa ctcagccgta tgctcgaaag catcgaccct
     4261 cggaccggcg agctggtccg tcggcgattg gaagccctgg cgcggcatcc ggacttggaa
     4321 gtgcgctgtc atgcctatca atctctgtta ttgtcccttc gggtgcccac ctatgattcg
     4381 atgctccgat cgttcgtcca ggcaggattg aagtttttga acgaggagac catcgagatc
     4441 atcgcgcagg aaaagcccga gcgcaggcgt ctcgaagcgt tccggcagcg tttgcacggg
     4501 tatcgttccc agcttccctg gccggcccct gacgatacac gaagcgtatt tattgatgtt
     4561 ttcaagcttt tggccagtct ggctcggtat catccggaat tctatggcgc cgtgcgagaa
     4621 gaactcgttg cgtggatcat gcatgagccg gcccctaagc tcgctgcggc ggcagagcga
     4681 gaattgcatg cgctggccgc tcgtttcgag cagtccctga cggggtcctg tgcggatccg
     4741 gcgagttggc agggtcgcat tgtgtttcag gatggcctcc atgccgaaga ggcggcgcaa
     4801 ctgcagcgca tcatcgtggg aaccagtttt ctcaaacaag ccatcatgct gtccaccgat
     4861 gaagaaacat gcgatatcga gcgaatcgtt cccgatggta tttgggtatc ccgaatttcc
     4921 acgttgcacc agcatgcctc gtaccgcgta agtatcaata cggatacggg aaagcattac
     4981 gatcttcaga ttgtcattcc caaggatatc agtcatgagc atgtgcttcg gacgatctat
     5041 tggatcattg ggattcgtgg atatcccttt gggcagccgg tattgccgaa attcgggtgt
     5101 tggagaccgg agttaggggc tctggcgctt gcttacgtca gtgatttgac ggtctgggag
     5161 cggatacggg cgtttgcgag ttttcggata cccggtacgg aatatcctcc ccccgaggcg
     5221 tggagaaagc tgtttgtccg cgcgatggca gcgtttttcg cgggttggcg agcgagtggt
     5281 cgtcgcatcg ttcccggcgc catcaatcct tccaacgtgg cggttccggg accggatttc
     5341 caggagggga cgcagattct atcccttacg gattggaaac cctacgatgg gccgaagacc
     5401 ctcgtggctc ccctggtgcg caacttttac gtacaaaccg tcagtcatta tccctggtgc
     5461 gccaaacatc tcgatccgga ttggatcctg caggcctgca tcgaagccat cggagaagaa
     5521 gaaggaacca attttttgcg agaattggat atcgtgatgg gcagcgaaaa gattccaggg
     5581 gcgggcattc cctggcatga ccggatcgag ccgtttttgt cgagattgca gacccatccg
     5641 ttcgttccat tggccgtatc ctgcgccgaa cagcgttacc ataagtggca acgggagaat
     5701 ccggatgcga cgagcacggc caaagagcaa tgcgtgttgg cggtacaagc gctttatggt
     5761 ttcgagcgct ttccggcctg ggttcgtttt cacctctatg cgcaaaccta ctttgcgaca
     5821 tttggcaagg aagtccagga tacctttcag cggctcatcc aagagatggc gcgcaatccg
     5881 ggacgcgacg ccacctcgtt gattgaggtt tccgacttgc aggcctggct cgagagcccg
     5941 gaagatcgcg ctgttttcaa ccgagcggta ttccccaaaa cccagcccac acaaccactc
     6001 gaatttctcg cggtccgcca tggcgagcaa caaaaggtga tccttcaaac gaaagtattc
     6061 gatgtcgatt ccgtacccta ttgcgtccgt gaacccctcg aaccgtcaga agtcggtaaa
     6121 ctctatcgcc actatgtcac ggccggattt cataaaacca tctcgaccca ggaccattat
     6181 ctcgtcttgc tcaacgagac ggaacagatc gtaggagggc tatcgtatcg acgcatggac
     6241 gacgatgtcg tgcatctcga tgggatcgtg gtatccggct atttgcgggg acgagggctc
     6301 aatggggcca tgttggagga tttttgcgca agaatggtga atgaaggcac gaaggtaatt
     6361 cgaacccact tcttcgttcg gcacttctac atgcagcgcg gtttccgtat cgatcgtcga
     6421 tacggtggac tcgtgagatt tctcacgaat gaatccactc ccgagcatcc tccctcctcg
     6481 atggtgctcc cgcacctgct cggcgattga acccaaatct ctgtcatgtt tttttggaca
     6541 ttcgtttcac agaacccttg agccgaggcg cgctcatcca tcttgacaga ctagggtgcc
     6601 gtggtaggtt ccgagatatg gtgagcaaca tcacgaatta gaggctcgcc cctacataga
     6661 tcccgaaacc tgcccgcgcg gagtgctccc atggcagaaa caacaatcct gctggtcgat
     6721 gacgacgatg atctgctcga agtcaaccgt ctcgccctcg aagcggccgg cttcggtgtg
     6781 cttatcgccc accatagtgc cgaagctttg gctatcgtcc aacagcacac gatccacggc
     6841 atcgttctcg atgtcatgat gcaaacccgt gacgagggat ttcatctcgc gcgggtattg
     6901 cgcaaaacac cccagacgcg caatgtgcct attctgatgc tcaccggcgt gaacgcggaa
     6961 agcgcgcgcc agggccatcc gctgcgtttg tccgacgatg accgagacga gatgtggctt
     7021 cccgtggacc ggttcctgga caagcccgtc tctcccgagc gtttgatcgc catgttgcgc
     7081 gagcttttgg gagaatgaaa tgagcaaaaa gccgggaccc catcgcccca gcgctcatgg
     7141 tcagcgcgtg caggaatctt ccgtgccctt gccgcgtccg cttctcgtca ggcttgcggc
     7201 ctaccaccaa attgcgactc agcatggcga ggagggaaat acaacgatcc gctcggggca
     7261 tatggccgcg cttttgaata tcgacccgac cctcgtgcgc aaagacatgg ctgcgctcgg
     7321 gatcaccgga aggccgaaag tgggatatcc gattggcacc ttgctgccct gtctcgaccg
     7381 tatcttgggt ttgacggaac gaaaatccgc cattctcatt ggttgtggcg acctcggcag
     7441 tgctctcgcc cgctatgggg gtttttcgag atacggcctg caaatctccg ctctattcga
     7501 tatcgaccaa aataagatgg gaaaaaccat cgccggcctc accgtccaac ccatggaaaa
     7561 atgtcgtcgc tacatcgaga ttttttccat acaaatcgcg atcttgaccg cccccagccc
     7621 ccaggcgcaa gacatcgccg attggctcgt cgcccgggat atcaaagcga tttggaattt
     7681 cgcccccgtt cacctcaaag cccccccgaa tgtcgtggtg cgcaatgaaa acttagcgct
     7741 tggacttgcc cagctcctgc accaaatcaa gcgctcgaaa accaccgccc ctgcggccga
     7801 acacgacgcc ttgcgccgcc gcgaaagtgc ttgacatgga aagcggaccc atcattgaca
     7861 cgctgcaaga cctttgcaaa cgttgttatt cctgtgtccg tcgttgtccc gccaaagcca
     7921 ttcgcgtgca aaacggccaa gccgaggtca ttgaacagcg ctgcatcggt tgcggtcgat
     7981 gtgtccatgc ttgctctcaa ggagccaaac gggtcgccag ccaattgact cacgtcgaac
     8041 agttgtgccg tcgggggcag accgtgctca tgttggcccc ctcgtttcca gcggccttcg
     8101 aaacctggtc cgcgcaccaa gtgatcgcgg cggctcggca agccggattt gccggcgtgt
     8161 atgacgtagc cttcggggct gatctcgtag cgcgccacca tcggcaaaga tacgaacggg
     8221 agcccggtcg tcccatcatc acctcggctt gcccagcggc ttgcgctttt atccaaaaat
     8281 acgccgtcga actcgttccc tttctggcac ccgtcctatc tcccatggct gccatgggaa
     8341 aagcgctaaa gacaatcgtg caaccgggct gctacacggt attcgtaggg ccttgctccg
     8401 ccaaaatccg agaaatcaga gatctggacg tcgccccttg ggtcgacgct gtcctcacgt
     8461 ttccggaact tgccgcgtta ttcgaaagac gttccatcga tccccgagtt ttgcccgaac
     8521 aggacttcga ccctcccttt gccggcctcg gcggcatttt tcccattccc ggtgggctcg
     8581 cccgagcggc ggaattgccc tcggatttgc tcgaaaacca cgtttttcag ctctccggaa
     8641 tcgatgaatt cgtcgatgga atcgaaagaa tgcgcgaacg agccacctgt ggggtcctgc
     8701 tcgaacaaga aacccggctt ttcgatgtcc tgttttgcaa aggttgcgtc gccggtcccc
     8761 tcatgccttc ccgaggaagc ttgctcgtac gcaaagaaaa agtcgttgcc ttcatgcgct
     8821 cgtacaccgg ccgacctccc tcgctcgaca gtgcgaccat gcggtcgttg ctggaaatgg
     8881 atatttcaag atccttcgac ccggacaacc agcgacggca ggagccgacg gaagagcaga
     8941 ttcgagagat tctcgcacaa acgggtaaat acaatccgca agacgaactc aattgccggg
     9001 cttgtggtta ccgttcctgc cgggagaagg ccgtagcggt gtatcatggt gtggccgaag
     9061 tggatatgtg tttgcccttt ttggtcgaaa aactcgtggc gacgatcggg cagctcaacc
     9121 gttcccatga ggaattgacg gaggcccatg cccaggtggt tcgggtggaa aaactcgctt
     9181 ccatgggaca attggcagcg ggaatcgccc acgaggtcaa caatcctttg ggttccatcc
     9241 tcctttattc gcaccttttg cgtgagtccc tcgaggggag catacccccg aatatcgaac
     9301 aagtgcgtaa cgatatcgac atgatattac aagaggccac gcgctgccgg ggcatcgtgg
     9361 ggggactgct cgacttcgcc cgacaatcca aggtttctcg ggccccagtg ctgctttcgg
     9421 acctcatcga cgataccata cgggtcagtc ggacgttcgc gagcagtcag gacttgcggc
     9481 tcgaaaaccg tagccccgcg ggattgcctt tggcgaacgt cgatcgggac caacttcttc
     9541 aagtgttgac caacatggta cgcaatgctt gtgaggtgat gcccgacggg ggaacgattt
     9601 ggcttcgtgc ggattggatt tcggagcgga atgaatttcg gatcatcgtg catgacgacg
     9661 gccctgggat tcccgaacaa cacatgaata aactatttac gccctttttt acgacgaagg
     9721 gcgtgggaaa gggtacggga ctcggccttg ccatctctta tgggattgtg aagatgcatc
     9781 gaggaagcat tacggcaaag aaaaacctgg atggtccggg ggcaacgttc gagatcaccc
     9841 ttcctgccgt tcattcccaa gccaatggca acggcaatgg acttttgggc gcggatactg
     9901 cggggcaagc accatgacag accttctttc gaccccggcg gagtcacccg cggcagaacc
     9961 cgaacgtcct acgattctcg tggtggacga tgaaatcggc tttcgagagg ggcttcgacg
    10021 gatcctgggc catcgtggtt atcgggtaga aaccgcttcc gatgggcaag aagccttgga
    10081 cctggcgagc caacgcgcgt ttggattggc tctggtcgac ctgaaaatgc cgggaatcga
    10141 cgggttcgaa gtgatcgaac gcctgcaaac gatcagcccg caaaccgttt gtatcgtggt
    10201 ttcggcgttt gccacggtcg aatccgctgt ccaatccacg aaaaaaggag ctttcgattt
    10261 cgtggtcaag cctttcacgc ccgacgacct gatggtggtg ctgcttcggg cggaggaacg
    10321 ttggcgcttg atgacggaag cggagcgttt gcgacaagag agagatagcc atctacttga
    10381 aattgcaaca gaaaaaggtc gtctccgcac gatcatcgac tcgatggccg atggaatcat
    10441 ggtggtgaac atcgacacga atatcgtgtt ggacaacccg gaagcacggc ggcttctcgg
    10501 gcgtgtatcg atgtcccgtc tcgagggcca ggtggcggat tacttcgagg acgaggcgtt
    10561 ggtacaagag attcgggcga tgttgacacg gggagacgct cttcccgtca cccgcgagac
    10621 caagctttcc acaccgggtc cggagcagcc agaccgctat ttacgcgcca cgctcgcgcc
    10681 catcaccgaa ggcccccctg acacccctcc gctcggtgtg gtggtcattc tgaccgacgt
    10741 gacccaggac aaagcgtttg agcggatgaa aaacctcttc gtttccatgg tggcccatga
    10801 actcaaagcg cccatcggcg ctgtggaatc ttacttggaa ttgattcgca tgggagccct
    10861 cgatggcgac cccgctcgca tcaaagccgt cgcagaacgc tgcctattgc gaacccgctc
    10921 gctgctcgac ctgattcgag acctgaccga aatgacccgt cgggaaggag gatttctcga
    10981 gcgccgcttg cagcgcatcg atgtatcggc cttcgtcgcc gaaattgtcg aattccaatc
    11041 cgcgcaagcc gcttcccgaa acatcacctt gcatttcgag gcgccgaaaa cgcctccgat
    11101 cctcgtggca gaccgcggcg atatcgagcg aatcatcacg aacttattgt ccaacgccat
    11161 caaatacaat ttgcccaacg ggcgtgtgga cgttcgactt gggacatcca gcggaacgat
    11221 cgtcctcgaa gtcacggata ccggggtcgg aatgtccccg gaagatgtcg ctcgtttggg
    11281 ggaagagttt ttcagagcca aaaacgccca cacccgtacc gtcacgggaa cgggcctcgg
    11341 catcgcgttg gtgaaacggg tcgtcgatag ctaccacggc gctctcgagg tcgacagtgc
    11401 tctgggcaaa ggcagcacgt ttcgcgtcat tttgccggcg gccccctcgg catcgatcac
    11461 aacgaaagga ggttccgtat gaaccttccg gactcaaaac aacaatccaa caaggcagag
    11521 cccgtacaag ctcaacccat gccaacgaca gccatggcgc ctgataaagt cattgtcgac
    11581 gaagcgcgaa tcgcccatac cctatcgagt tgtggcggct ccgatcccgt gcatatccgc
    11641 gaaatcatcg ccaaagccaa agagttgcgg gggctcgacc tcgaagatac cgctgcgctt
    11701 ctcacctgcg atgacccagc cctgctcgaa gaaatctttg cggccgccag ctatgtcaaa
    11761 gacgccatct acgggcgcag ggtcgtgctc tttgcccctc tttactattc cagcacatgc
    11821 gtaaacaatt gtttatattg cggttttcgc agagacgcca agcgttcgcg tcgcaccctt
    11881 tccctcgaag aggtggcgcg ggaggtgggc gcgctcgaac gagaaggaca tcggcgattg
    11941 ttagtgattg gcggtgaaac ccccggcccc aaagcgatcg gtcgtttgat ggacgtcatt
    12001 cgcaccgtgt acgagacgcg agatgggggt gagatacgac gtgtcaatgt ggaaacggcg
    12061 ccgatgacga tcgaggagtt tcggcagctc gcccaggcgc gtatcggcac ctacgtggta
    12121 tttcaagaga cctatcatcg ggccacctac aaggccatgc atcccgatgg gcccaaagcg
    12181 gattacgatt ggcggatcga agtcatggat cgggccatgc gcgcgggaat cgaagatgtc
    12241 ggcatcggcg tgctgtacgg cctgtacgat tatcgcttcg acacgcttgc gctgctcgag
    12301 catgcgcgtc atctcgaaac ggtccacggc acggggccgc atacgatttc cgtacctcgg
    12361 gtggaaccca cgcccgatgc tcctctttcc ctcgctcccc ccgctgctct taccgacctc
    12421 gacctttgca aagtcgtggc gattctccgt ctttccgtcc cttacaccgg aatcatccta
    12481 tcgacccggg agcctccgca attgcgggac aagctgctgg accttggtgt ctcacaaatg
    12541 agtgccggtt cctgtaccga acccgggggg tatgccggcg aacatgattc cgcgagccaa
    12601 tttcctgtcg cggatacgcg ctcgttggac gacgtgattc gctccattgc ccatcatggc
    12661 tatttgccaa gcttttgcac gggctgctat cgcaagggaa gaaccggggc ggacttcatg
    12721 gagcttgcca agccgggtct catccgcatg cattgcctgc ccaatgcctt gttcacgtat
    12781 ctcgaatacc tcatggatta tgcgtccgag gcaacgcgac aagtcggtct cgacctcatc
    12841 gaaacccagg tcgagcatga agtgcctccc gctcgacgca acacggtgcg caagatgatt
    12901 gagaggattc gagcggggga aagagaccta tatctataac cgtcgccccc gtgacgattg
    12961 gccctcgcat cgataactat cattccctcc gctcgcccac tgttcttacg ccatcttcct
    13021 tgcgtgagcc tcgacaacca tgagcgtgcg tgtcccacta gcaggtgcca acccgtttga
    13081 ctcgtcgcgt ctttgacgac aaactcaggg actacctttt tctccaacga tggagcctga
    13141 cgtgcgaacc ggtcacttgc tcatgctggc ggcgttgtca tcgattccca tcggttgcat
    13201 aggggactcg ttttcatcgg aagaacctgg ccccatttct tcacctgacg cttcttcgga
    13261 tggttccgac gcttcttcgg atggttccga cgcttcttcg gatggttccg acgcttcttc
    13321 cgacgccgtt accgagcgtg aacccgacgt tcctatcgac agtatgccgg aggatgggag
    13381 cgacgaaacc gatcaaggcg ggaatgaagc ggggaaaacg gcgactggcg ccgcgtgtaa
    13441 cgaggacgac gagtgtgaaa ccaacatgtg tcaggacggc gtatgttgtg aatccgtctg
    13501 cgatccctgt ttttcctgtg gacagccagg tcacgagggg cagtgcttcg ctgtccccga
    13561 cggagaaaac cacaacgagg cgtgtggacc ggctggttca ccatgcatgg gagtttgtga
    13621 tggctccggt cattgtactt atccggtggg agccaattgc gatgaccccc attgcgacag
    13681 cgacccgtgg aaacaagttt tgtttcaatg caatgccgag ggactctgcg aggaacaaat
    13741 cgtttcttgc tccccgttcc tatgcaaaca agccgaatgc ttgagcaagt gcgacgactc
    13801 cgacgattgc gaggatgacg cgtattgtgc ctcgtcggaa tgtgttccga agcttgtctt
    13861 cggacagacg tgtactcaaa acgtcgaatg cctttcgtcc cattgcgccg atggtgtttg
    13921 ctgcaacgac tcatgcagcg gagtctgtga aacctgtgca ttcgcaggca aggagggaca
    13981 gtgtgtggct gtcccattgg acatggatcc cgacgacgag tgtcaggggg atggatcgac
    14041 atgcgcggga acgtgcagtg gtcagcgttc ttgcgttttt cctgtccacg gcgagccctg
    14101 cggggaatcc caatgcgatg cgaacctgaa cgctgcccat gaactgattt gcaatggcga
    14161 ggggaaatgt gtcgagaacg tacaaaactg cgggttgtac gattgtcata aggacatcgg
    14221 gtcttgtctt tatagctgta gtcaggatca ggactgcatc gacgatgcgt tttgtaataa
    14281 cgacgtctgc gttgccaaaa aatcgaatgc ctcgccttgc gagaatagca tcgagtgttt
    14341 gagcggatac tgcgagccca ccagccacgg gaagcagtgt tgcaacacgg cctgtccgca
    14401 tcccttgaac tgctcgactg gcgaatgttt gtgtgacact gtcgtctgca gtcccggtgt
    14461 gaattgcgtc ccatggtatc gcgatcgcga tggcgacacc ttcggcaacc ccaacgagat
    14521 gaagttgggt tgtgagaatg cgccaccgaa tatccccggg gagaaatacg tccggaacat
    14581 ggatgattgc tttgatttga accatctcgc gcgaccgggt caaaccgcct ttttcccaga
    14641 acaccgtggt gatggcagct tcgactatga ttgcgatggc aaagaaacga agcagttcga
    14701 cgacacgaat gaccccgtct gcaaggattg taaaacctac tcgaaatgcc tgtattgcgg
    14761 gatcttttcg tataatacgt acggttatcg atgcaaaacg ccaacgtcat cggacaacac
    14821 ttgtggtccg ttggttacgc aaagttacaa gcaattgcgg ccctgcggac aatcgggaac
    14881 tctgtatcgg tgcaacaacg acgacaaact gtgcgacact gctgaggtgc ccactcacga
    14941 cgtcgttcaa caatgccgat gatgcgcgag cttcgtttcc attcacggct tcgttggggt
    15001 ctgcgacggc tagtgctcgc atgggttttc gtgatccacg tggcccatgc acaaacggca
    15061 tccgaagttg aacaggccaa gcgattgttc gccgcggcgg aagaggatca agcgaacctg
    15121 cgctgggcgg aagccttgga aaaactcgag caagtcgtaa tgatcaaaga cacggcgggg
    15181 gtgcgtttcc atatcgccgt ctgtcaagat cgattgggtt tgcccgcacg ttccttggaa
    15241 aacttcgaac gagcgcaaca gcttgcgctt tcttcgggaa gcgacgacgt attggaattg
    15301 gtgggtccgg aaatcgaacg gttgcgccag cgagtagcga cgttgacgat cgaggtttca
    15361 tcgcgggttg tcgatgttcg catcgaccgg aagcggaagc tgccggcgac gaccttgctt
    15421 tacgcgc
//
LOCUS       DAOSOR010000057        15275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_3904_length_15275_cov_3.756965, whole genome shotgun sequence.
ACCESSION   DAOSOR010000057 DAOSOR010000000
VERSION     DAOSOR010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 15275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15275
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3904_length_15275_cov_3.756965"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2064)
                     /locus_tag="PK710_04850"
     CDS             complement(<1..2064)
                     /locus_tag="PK710_04850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOT09075.1"
                     /translation="MTKLTEDQPSTPNTTQASCAGESTRNAPSSWLLRAAGLLIPLLI
                     LMPSLGSGGIWDPHELNVADFARRIGINLFGAQALSLADADNTMPTLEQLGKGELPFT
                     SIAAGFAAFGLHEWAGRLPLALWALAGAGAIYWLVSRLMDERAGVFASIALSTMPLFF
                     VQARTMLGDIVTFSSITMAVAGFGLLVFDNQPTRRSQVVSTLLAILGGTAGFLSRGIL
                     IGLALPLLTVGLAWLVCRLSNGATKSNRLSTPVAIAILVLGSGAAAWGVYTLVTAQGS
                     QAVRVLGAIVTRPSQPPSFDHVLLMLGHALFPWSAFIPFAVGRLFRPAFALQDSQDAR
                     ERALRVLLIVGTSLSFGILGLMNVYVGHIPFAGIGLMAAIAAVAIRDLERGGHASRAM
                     AIGTVTFLALLYSDFKKWPDKAFSAYGIESTSFPDSFKQDAARLMLVCTIVCGVLVFA
                     SWLERDAQDRKPFVFSDYFGILKALRTAAKGNLVIVGGVVELALVVTAANVFIGMKAQ
                     WKSVVTMSATVRMIALNAWWAVPVALLVALALALAFRDINRWFFPRFRVSRASATLAA
                     GALGGAILSFSYFPALAAQLSPKEVFDSYAKMRSGQEPLALLGVSSRTASYYAGGAID
                     SFSDVEGALQWLLDGQERKWLVLRNEDLARLNSSYRARTRAGRNLPILDARSSQILLA
                     SNKLNN"
     gene            complement(2146..2658)
                     /locus_tag="PK710_04855"
     CDS             complement(2146..2658)
                     /locus_tag="PK710_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09076.1"
                     /translation="MIHPDTKAQNSSVPTISRLRFRVALVFVFLGMAVLVYHRITHIP
                     PPAPKPVIFGLQGPETPAAPDVLRLLDDLRPGDKLGDGTVLAIDAPVERIVWVDVQLG
                     SRVFGIGVGATGTGGDKPMPITTEKYEVRHGMLRGEGEFNPQAMQQAAEQLAARIRRR
                     EQTVPLPSGM"
     gene            2802..3677
                     /locus_tag="PK710_04860"
     CDS             2802..3677
                     /locus_tag="PK710_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09077.1"
                     /translation="MRKLIGTSLIAACCLAVPLLTASPAQAGLEACGNINVEASAQCK
                     VEVEGGCVAKCTPVSFYASCSAKGYASCEGKCDLPAVDCSASCEGSCQGSCEPNPNFS
                     CEANCTGSCDADCSGQCAAAGNKGECEAQCKATCKGECDASCKGSPATCEGKCQGSCE
                     GQCKAKANMSCQAECQGKLSANCQSELKGGCEAACKKPEGALFCDGQYVDHGGNMKDC
                     IAALNAMLTVKVDASASASLECANGKCEAEAEAEAGCSLSVANQPSDGSAPYALGLLG
                     ALGALVFGRYRRRQK"
     gene            3637..4293
                     /locus_tag="PK710_04865"
     CDS             3637..4293
                     /locus_tag="PK710_04865"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOT09078.1"
                     /translation="MPWCSDATAVDRSKTLNPPFLLQARRFVSPHGWGGALALVVDQL
                     SKLYAEKSHLLHPPVPTMAHHRASSETAWLWVSDSGNSYFAFHVTHVANAGIMMGALE
                     NAAPPIPWLMFMATTVIGLAMGIGLLAIAKQSEKGLLWGAVLLTAGVASNLMDRLRVD
                     YVVDWAHLQWRLGGWLVDAPAFNLADLFIIAGSALASIALIRRKLPLAHVHQSQIKQG
                     "
     gene            complement(4249..5133)
                     /locus_tag="PK710_04870"
     CDS             complement(4249..5133)
                     /locus_tag="PK710_04870"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOT09079.1"
                     /translation="MTLDSRACPKPRRFAISHVIMGLARTSRPLGWIAALALYRIGMA
                     YGAVQDSTVTLLLSIVITFPFCLYLFGLNDLADQDSDADNPRKGNWIHGASSPIPHTL
                     SRWAPWIGGAMVASIIPWLPLAAALLLSGILFLSWAYSSPPIRLKEIPIVDGLVTASI
                     MIGLLCLGHLSGPSSLPLATETFTVAPTLVGLHIFASVVDVDADKTAGHRTLAVRTSP
                     RFAGIVALALSLVSTSTIPWLDYSPPITAYLILQPIVFVVWMLFGRRFTGHRAISLVG
                     LAGLLTLLYLALVYVRQR"
     gene            complement(5161..5910)
                     /locus_tag="PK710_04875"
     CDS             complement(5161..5910)
                     /locus_tag="PK710_04875"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT09080.1"
                     /translation="MLVRKLSITAVALFVTTTLMGCEAPPPEPSSKTASPSETPKPPP
                     SSTPTAPAAQPAKPIAPALLAPEKATETAPASFKVKFDTTQGQFVVEVTRAWAPKGAD
                     RFYNLVKIGYFEDIAVFRVIDGFMAQFGIHGEPKVNSAWRNARIDDDPVKESNKRGYL
                     TFATSGANSRTVQMFINFGDNSRLDSQGFSPFGKVITGMDVVDKLYKGYGEGAPVGDG
                     PEQGRLQFQGNSYLKSSFPKLDYIKTATLTP"
     gene            6126..6947
                     /locus_tag="PK710_04880"
     CDS             6126..6947
                     /locus_tag="PK710_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09081.1"
                     /translation="MAESTDPSEPHGFSGHASLARRLCRVVPSLSTQALRMAYVRSVW
                     IESDSSEFAWAIDAVCEWSKARDKRAQEVMLAFAALLHEPSLRCRIESLRHDAEQGKL
                     GSLGSLLPSGEDSKKEQSRGEVDERHVPDYGYGRPLTLGERKSMARRPSRRQLDRLLA
                     DPHPSVILNLLNNSITTEDDVVRLAAKRPLRHEIMKEIARHPKWNVRRRVRLALVLNP
                     GCPPELGIPLVELALRSELRTVMERTEIHPDIRRAALARLGEGRSDAHGDGSKKS"
     gene            7084..7542
                     /locus_tag="PK710_04885"
     CDS             7084..7542
                     /locus_tag="PK710_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09082.1"
                     /translation="MVWSNSGWTALALGYFVLALGCASGDGVPTATIENGLDTCIVEA
                     SYLEYGFSNPIGPSGRMAPREVREGSAVAYAVVMAPEPGENCVDLAGKRGGSLWITHG
                     AFEAKAGELTSIRFSKDTASEIPTECSPQYVEGLKRFSRLKDACDPNAGK"
     gene            7575..7820
                     /locus_tag="PK710_04890"
     CDS             7575..7820
                     /locus_tag="PK710_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09083.1"
                     /translation="MSVGGGPLATMRQLVVDMVDMVTDPDYRLSWALKVGGVVALVCL
                     ALPSDLRVPGYIDLVFVLGLLWRERSKYLDFVHKMGQ"
     gene            complement(7873..8127)
                     /locus_tag="PK710_04895"
     CDS             complement(7873..8127)
                     /locus_tag="PK710_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09084.1"
                     /translation="MSSQRNAVPPSPHLIIAAVKGDSLPKEPLYCDVCGKIIEGEPAG
                     KGLLLWTRGDEVREELPVLCETCAPAIAAAGGMKWIEEEE"
     gene            8250..10091
                     /locus_tag="PK710_04900"
     CDS             8250..10091
                     /locus_tag="PK710_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT09085.1"
                     /translation="MPTLRTYLPKGQPRLHSVNKPITTIGSSPSNDIVVQADGVAEAH
                     AQIIFDGRFFNLEEVDRAADIQINGKKKRRARLSHNDRIHLGSAEIAFSLLGDPSFVL
                     ATEQETSSNTTDIAGLEQLNAFSERLMQRGSVDELLTDLLDAVVEISGAHRGAVLLVE
                     PGEPSDTERRVSVRAVRNVRRDIIADASGGISDSIVRKVITTGMPVIVSDALADQQFG
                     KSESVLALKLSSVMCVPMLAQGESLGALYVGNDHVKQLFDRSQLDLLTVFAAQASLIL
                     QNAMLLSSLRADKEKLQEELSDMRFGEIIGACDSMLEVFRKLQKVASTDISVLITGET
                     GTGKELIAREIHRRSPRVRGPFVTINCGAIPENLIESELFGHVRGAFTGAVASRPGKF
                     QVADKGTLFLDEIGELPLNLQVKLLRALQEHVVYRVGDSRPEKVDIRIVAATNRNLEE
                     EIRENRFREDLYYRLNVVNLWLPPLRARGEDVVLIARALLSKYADELNSSVRGFAPDA
                     LTAIKRYAWPGNVRQLENRIKKALVLCDHNLLTADDLDLVREEQSPILPLERAKEEFQ
                     HRYVREVLERNNGNRTQTARDLGVDPRTIFRYLEREQNPIPSGGGKG"
     gene            10415..11107
                     /locus_tag="PK710_04905"
     CDS             10415..11107
                     /locus_tag="PK710_04905"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOT09086.1"
                     /translation="MRIWTRAPILFSLTISLLCAVPLAHEARAEVAAHSAMDANAIAT
                     RVQAFYDGTRTYQARFEQVYHIEAYNRKKRSAGQVIFQRPGRMSWKYDEPNGNRVVSD
                     GQNLTIYERDKSQVVEQPLGTSQYPAVLSFLVGKGNLREMFHLRKLDEKRMNFEGGWV
                     LEGTPKQATSAYSKVIMYIDAETAQVRRMLVIDAQRNRNLFTFSNPTVNTAVPTGAFV
                     FKPPPGTQVVRP"
     gene            11492..12829
                     /locus_tag="PK710_04910"
     CDS             11492..12829
                     /locus_tag="PK710_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09087.1"
                     /translation="MFRGFIGVVLTVLVLGVSFAASASGFDLFGVQAQGIAEVSARTA
                     AARDGSASYYNPGGLALGQGYEVDLSALGVMSQLEVQDRSYAIDEPWGATVTVAAAVP
                     LQGPLANRIRLGLALQGFGNTLMKLKLREASRPFFPYYDNRTQRLVVIPALSVRLAEG
                     VGLGLGINTLAGMQGPIDVREGQSRMMEARVVQEAGTVARWIVGVQVEAMEALHLGMT
                     FRQSFGVPLSITTTADVAGIPLVVDISSAEALFDPMMVVLGAKVEPSADASMELDIAY
                     HGWSRWKGPWLGLDTTVSALSISGKLPEGLFRDTMSVRGAVAWTVERASERITSLHGG
                     LGYETSMLDRGEQQGRTNLVDGAKVMVGAGVTVLFPRVLGQGFRAEIGMQMHQVGLYR
                     QEKIACTRLPCPAGTVFGSDATAPDTDITNPGYPVLTGGGRVFVGTVGMGVDL"
     gene            12826..14103
                     /locus_tag="PK710_04915"
     CDS             12826..14103
                     /locus_tag="PK710_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09088.1"
                     /translation="MRLGGLIVVGLSMMTMARPAAATAGYLFGWGPRDISLAQSDVAD
                     AASVAAAWVNPSLAWEPGARMAVGYVYGWTALEWNGERVKLRDIAGTQVGFQLGGELG
                     SDIALGGAVVMHIPDRSLARVSFLPGTEPVFFRFDPASQRTTMDAVLALRLGWLSIGV
                     GGEVFVSAQGDVDFVLGQDANGTYADGEAQVTLAYDVTWLAGLTMDLGAAKLGLRYRG
                     AQALDVDLSTLATVDVQGNPLNGTTKVRIVGRSGYVPATVDLGASWTVGAVGHVMTSL
                     QYARWSDAPSPVAALSMDVDLGLSPGLRQGSFVMPGFRDTVSPRVGMEMEPWGEEAVA
                     LRAGYQYSPSPVRVSRGFASPMDASTHGFALGAGVDCGRVWGVDIRSDAAWQWLMLER
                     RTLDKPGDALPFSHSEVSGSVKLMSVSVQGGWL"
     gene            14100..>15275
                     /locus_tag="PK710_04920"
     CDS             14100..>15275
                     /locus_tag="PK710_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09089.1"
                     /translation="MKRWVGMLFVGLSLACGLLVPEDLTESARRDEYSGFVEGGAIQV
                     CFDGVPLISPALAKEGPLSICMDRQGDERRCGPGAGCGSGERCICGLCSTRPCRSATE
                     CEGGEVCQSNRCTKSCGDNRKCGEGERCEAGACVRPCMSHGQCAHGERCSTFDGTCVV
                     KLCGEQVGCGGSEVCVARQVVADVREPTVVSFDEGKVAYVEVRGGPAYPNACAVYRVR
                     VQEMNRWVIEPSTPVLVPREGDGGCLGGPSVLRGGGGVVMVASRGDGSGIVKATSRDG
                     VNFDSDAGVMLEPALQWERGWVGAPALVAWRGKWLMLYEAGRGAGIGIANVSESGVVR
                     HSNEPWLVPSHFEDPSVWRDVQRIGSPYGIEREGKLWVYLTVRGVEGLDAVSRQGQRY
                     "
BASE COUNT         3156 a         3579 c         5166 g         3374 t
ORIGIN      
        1 gttgttcaac ttgttggaag caagcaaaat ttggctcgaa cgggcgtcga ggatcggcaa
       61 attgcgaccc gctcgcgttc gagcacgata ggaagaattg agccgggcca agtcctcgtt
      121 gcgcaacacg agccacttac gttcttgccc gtcgagaagc cattgcagtg ccccttcgac
      181 gtcggaaaac gagtcgatgg cgcctcccgc gtagtagctt gccgtacgac tgctgacgcc
      241 aagcaaggcg agaggctcct gtccagaacg cattttggcg taagagtcga aaacttcttt
      301 cggcgacagt tgcgcggcga gagcaggaaa atatgaaaaa ctaaggatgg caccgccaag
      361 ggctccagcc gccagggtgg cggatgctcg gctcactcga aagcgtggga aaaaccatcg
      421 attgatatca cgaaacgcga gggccagagc cagagcgact agcaaggcca cgggtacggc
      481 ccaccatgcg ttgagcgcaa tcattcgcac cgtggcgctc atggtgacca cgcttttcca
      541 ttgtgccttc atgcctatga aaacgttcgc ggccgtgacg acgagagcga gttcgacaac
      601 cccccccacg atgacgagat tgcctttggc ggcggtccga agagctttca atatgccaaa
      661 atagtctgaa aaaacgaagg gtttacggtc ttgcgcatct cgctccagcc aggaggcgaa
      721 aaccagcaca ccgcatacga tcgtacaaac gagcatgagc cgagcggcgt cttgcttgaa
      781 actgtcgggg aaactcgtgg actcgatgcc gtacgcgctg aacgccttgt ctggccactt
      841 tttgaagtcc gaataaagga gggccaaaaa ggtgacggtt cctatggcca tggccctgga
      901 agcgtggccg ccacgctcga gatctcggat ggccaccgcc gcaatcgccg ccatcaggcc
      961 aattcctgca aagggaatat ggccgacgta cacgttcatc aagccgagaa ttccaaacga
     1021 caaactcgtg ccgacgataa gcaagacacg aagggcgcgc tcgcgagcgt cttggctgtc
     1081 ctgcaatgcg aaagctggtc ggaatagcct gcccactgca aaaggaatga acgcgctcca
     1141 aggaaaaagg gcatggccga gcatcaatag aacgtgatcg aaggaaggag gctgagaagg
     1201 cctcgtgacg atggcaccta gcactcgaac ggcctggctc ccttgcgcgg tgacgagcgt
     1261 gtaaacgccc cacgcggcgg caccggaacc gagcacgaga attgcgatcg cgacgggggt
     1321 ggagagacga ttcgatttcg tggcaccatt cgacaaccgg catacgagcc acgcgagtcc
     1381 gacggtaagc aggggcaaag ccaatccgat cagaattccc cgagacagaa agccggcggt
     1441 cccaccaagg atggcgagca gggttgagac aacttggctt cggcgcgtag gttggttgtc
     1501 gaaaacgagc agaccgaatc ctgccacggc catcgtgata gaggagaagg tgacgatgtc
     1561 tccgagcatg gttcgcgcct gcacgaaaaa aagaggcatg gtggatagtg caatgctggc
     1621 gaacacgcct gcccgttcat ccatcaatcg gctgacgagc caataaatcg ctcccgcccc
     1681 cgcgagagcc catagcgcga gggggagtct tcctgcccat tcatgcagtc caaaagcggc
     1741 aaatcccgct gcaatggacg taaacggcaa ctctccttta ccaagctgct ccaacgtcgg
     1801 catcgtattg tccgcgtcgg cgagactgag cgcttgcgca ccgaaaagat tgatcccgat
     1861 gcgtcgtgcg aagtcagcga cgttgagttc atgcggatcc cagattccac ccgaccccaa
     1921 agagggcatg agaatgagaa gagggatgag caagccggcg gcgcggagta gccatgaact
     1981 aggcgcgttt cgcgtgcttt caccagcaca agaggcttgg gtggtgttgg gtgtggaggg
     2041 ctggtcttcg gtaagcttgg tcatggtctt tttacggggg acgacgcccg acagggctgc
     2101 actgtagtca aagaggcgtg ccttgcaacg aatggtggtg gaagattaca ttcccgaagg
     2161 cagcgggacg gtttgttctc gtcgtcgaat tcgagccgcg agttgttcag cggcttgttg
     2221 catggcctga ggattgaatt cgccttctcc gcgcagcatg ccgtgtcgaa cctcgtactt
     2281 ttccgttgtg atgggcatag gcttgtcccc accggtgccg gtcgcaccaa ctcctatccc
     2341 gaacacccga cttcccagtt gtacatccac ccacacaatg cgctcgaccg gtgcatcgat
     2401 ggcgagcacc gtgccatcgc ctagcttgtc tcctggccgt aggtcgtcaa gaagccgaag
     2461 aacgtccgga gcggctggtg tttcaggacc ttgaaggcca aaaatgacgg gtttcggcgc
     2521 agggggggga atgtgggtta ttcggtgata gacgagtaca gccatgccca aaaaaacaaa
     2581 gacgagcgcg acacgaaacc gcaaacggga aatggtcggg accgatgagt tttgcgcctt
     2641 cgtatcagga tgaatcatgg cgttgacagg gctccgctgc tattgttttg gacatccgtt
     2701 gaagcaatgg agggaatgtt cgtctatctt cgacgtaaga tttcagcaag ccctgggata
     2761 tcacccccct gctacttggg ttaatcatta aggagaaggc aatgcgtaaa cttattggaa
     2821 ccagtctcat cgccgcctgt tgtcttgccg ttccgcttct gaccgcatca cctgcgcaag
     2881 cagggctgga agcatgcgga aacatcaatg ttgaagccag tgcccagtgc aaggtggagg
     2941 tagagggcgg atgcgtggcc aagtgcactc cggttagctt ctatgcctct tgttccgcga
     3001 aggggtatgc atcctgtgaa ggcaaatgcg acttgcccgc ggtggactgt agtgcctcgt
     3061 gtgaagggtc ttgccagggc tcatgcgagc cgaatcccaa tttttcctgc gaagcgaatt
     3121 gcactgggtc ctgcgatgcg gattgctccg ggcagtgcgc ggcggctgga aacaaggggg
     3181 aatgcgaggc gcagtgcaag gcgacctgca agggggaatg cgatgccagt tgcaaaggct
     3241 cgcctgctac ttgcgaagga aagtgccagg gaagctgcga agggcagtgc aaagccaagg
     3301 cgaatatgtc ctgccaagct gaatgtcagg gcaagctatc tgcaaactgc cagtccgagc
     3361 taaaaggtgg atgcgaagca gcgtgcaaaa aacccgaagg cgctctgttc tgcgatggtc
     3421 agtacgtgga ccatggcggc aacatgaaag actgtatcgc cgcgctcaac gccatgctga
     3481 ctgtgaaggt cgacgcttcc gcgagcgctt cgttggagtg cgccaacggc aagtgcgaag
     3541 ccgaagccga agccgaggca ggatgtagcc tgtctgtagc caatcaaccg agcgacggta
     3601 gtgcgccgta cgctttggga ttgcttggag ccctcggtgc cctggtgttc ggacgctacc
     3661 gccgtcgaca gaagtaagac tctgaacccc ccttttctcc tccaggctcg gcgcttcgtt
     3721 tcaccacacg ggtggggcgg agcgctcgcc ctcgttgtcg atcagctttc caagctgtat
     3781 gcggaaaagt cccacctcct tcatccaccc gtgccaacca tggcgcatca tcgtgcgagt
     3841 tccgagacag cctggctatg ggtttctgat tcgggaaatt cgtatttcgc ctttcatgtg
     3901 acccatgttg ccaatgcggg gatcatgatg ggggctttgg aaaacgccgc cccacccatc
     3961 ccttggctga tgttcatggc aacgacggtt attgggcttg cgatgggaat aggactgctg
     4021 gctatagcca aacagagcga aaaggggttg ctctggggtg cggtgctgtt gaccgcaggt
     4081 gtcgcgagca atctgatgga tcgtcttcgg gtggattatg tcgtggattg ggcgcacctg
     4141 cagtggcggc tgggaggatg gctcgtggat gcaccggctt tcaacttggc ggatttgttc
     4201 attattgccg gaagcgcctt ggcctcgatc gctttgatac ggcgaaaact acctttggcg
     4261 cacgtacacc aaagccagat aaagcagggt tagcaggccc gccaaaccta ccaaagagat
     4321 ggcgcggtgt cccgtgaacc gtcggccgaa aagcatccag acgacaaaga cgatgggttg
     4381 caggatgagg taggcggtga tgggaggaga gtaatcaagc caggggatgg tggatgtgga
     4441 gacaaggctg agggcaagag ccacgatgcc ggcgaatcgg ggagaagtac ggaccgccaa
     4501 ggtgcggtgt cccgccgttt tgtcggcgtc cacatcgacg acgcttgcga atatatgaag
     4561 gccgacgagg gtgggggcga cggtgaaggt ttcggtggcc aggggcaagg acgacggccc
     4621 ggatagatgg cccaggcaga gcaagccaat catgatggat gcggtgacga gaccgtcgac
     4681 gatgggaatt tctttgagcc gaatgggggg cgatgagtag gcccaagaaa gaaataggat
     4741 cccacttaat agaagagcag cagccagggg aagccaagga atgatactgg cgaccatggc
     4801 gccgccgatc caaggagccc atcgagacaa tgtgtggggg atggggctgc ttgcaccgtg
     4861 gatccaattg ccttttcgtg gattgtcggc atccgagtct tggtcggcga ggtcgttgag
     4921 accaaagagg taaaggcaga acgggaatgt gatgacgatg gacaggagca gggtgacggt
     4981 ggaatcttgg acggcaccgt aggccatacc aatgcggtac agcgcgaggg ccgcgatcca
     5041 tcccagaggg cggctggtcc gtgccagacc catgatgacg tgagaaatgg cgaagcggcg
     5101 aggtttgggg caagcgcggg aatcgagtgt catggtggac gaaccgcgct tcacgatagc
     5161 ttacggggtc aaagtggcgg ttttgatgta gtcgagcttg ggaaaactgc tcttcaagta
     5221 ggagtttccc tgaaattgca ggcgtccttg ctctggaccg tcgccgacag gggccccttc
     5281 tccgtagcct ttgtacaatt tatcgacgac atccatgcct gtgatgactt tgccgaaggg
     5341 agaaaatcct tgggagtcca ggcggctgtt gtcgccgaag ttgatgaaca tctggacggt
     5401 tctggagttg gctcctgagg tggcgaaagt taggtagccg cgcttgttcg attccttgac
     5461 gggatcatca tcgattcgag cgtttcgcca ggcggagttg accttgggtt caccgtggat
     5521 tccgaattgg gccatgaagc catcgatcac gcgaaagaca gcgatgtcct cgaagtatcc
     5581 gattttgacg agattataga accggtccgc gcctttggga gcccaggcgc gggtgacttc
     5641 gacgacgaac tgtccttggg tggtatcgaa tttgactttg aaagaagctg gtgctgtttc
     5701 ggtggccttt tcgggggcaa gcaaagcggg agcaatgggc ttcgccggtt gcgcggcggg
     5761 cgcggttggc gtggaagacg gtgggggttt tggggtttcg gagggtgagg ctgtcttgct
     5821 ggaaggctcg gggggaggcg cttcacaacc catcaatgtc gtcgtgacga aaagggcgac
     5881 cgcggtgatg ctgagctttc gcacaagcat gaatacactc ctcatatagg gataattcgc
     5941 tgtgatcacg gcatgcgtga gcagcgctgc gcatcctagc caatggctcg cggctgtcaa
     6001 tgcggcgcca tcgatcgcgt cgaggtgaac ggtggccggc ttcaaccacc tgaaggtatt
     6061 ggctggaagc tttggcccca aggacgacaa cctacaaggg cacgaattgg ggtacactgc
     6121 cgtgtgtggc ggaatccacc gatccttctg agcctcatgg tttctcgggc catgcatcgc
     6181 tcgcacggcg tttgtgtcgt gtggtgccgt cgctttccac gcaggcgctg cgtatggcgt
     6241 acgtgcgcag cgtatggatc gaatccgact cctccgagtt tgcttgggcg atcgatgcgg
     6301 tatgtgagtg gagcaaagcg cgtgacaagc gggctcagga ggtgatgctc gcgtttgcgg
     6361 cattgttgca tgaaccaagc ttgcgttgtc ggatcgaatc gcttcgccat gacgctgagc
     6421 agggcaaatt gggttcgttg gggtcgcttc tgccctcggg ggaagactcg aaaaaagagc
     6481 agagtcgtgg cgaagtggat gagcgacacg ttccagacta cggttatgga cgaccattga
     6541 cgcttggaga gcgcaaatcg atggcaaggc gcccatcacg tcggcagttg gatcgattgc
     6601 ttgccgaccc tcatccgagc gtcattctga atttgctgaa caacagcatt acgacggaag
     6661 atgatgtcgt gcgattggcg gcgaagcgcc cgctgcggca tgagatcatg aaggagattg
     6721 cccgccatcc gaagtggaac gttcgtcgtc gtgtgcggct cgcgcttgtg ctcaaccctg
     6781 gttgtccgcc ggagcttggg attcctcttg tggaattagc gttacgttcg gagcttcgga
     6841 cggtgatgga gcgcacggag attcacccgg atattcggcg tgcggcgctt gcgcgtcttg
     6901 gggagggccg ttcggacgcc cacggggatg ggagtaaaaa atcgtgaggg cagccgaagc
     6961 acaagcatgg tggatggcct atgttgggcg gtccaggcaa ggacttggga aggcaggaag
     7021 gacgctgatt gcgcccaggt actacgcctg ctatgggagg cctttatggg agagtcccgt
     7081 ttcatggtct ggtcgaacag tggatggacg gctttggcgc tgggctattt tgtcttggca
     7141 ttggggtgtg cgagcggcga tggggttccg acggcaacca tcgaaaacgg gctcgacacc
     7201 tgcatcgtgg aggccagcta tttggagtat gggttcagca accccatcgg tccttctggg
     7261 cgaatggcgc cccgtgaggt cagggaagga agtgctgttg cgtatgctgt cgtgatggca
     7321 cccgagcccg gagagaattg tgttgatttg gcgggcaaac gaggtggttc gctttggatt
     7381 acccatggag catttgaagc gaaagcgggg gagcttacga gcattcgctt ttcgaaggac
     7441 acggcgtcgg agatccctac ggaatgctct ccccagtatg ttgaagggtt gaaacggttt
     7501 tccagattga aggatgcgtg tgatccaaac gctgggaagt agccgatgcg agaaaatcca
     7561 agggtcgttg gcggatgagc gtgggagggg ggccgttggc gaccatgcga cagcttgtcg
     7621 ttgacatggt cgacatggtg acggacccgg attaccggtt gagttgggct ctcaaagtcg
     7681 gtggggtggt tgcgttggta tgtcttgcat tgccgtcgga cctgcgggtg cctggatata
     7741 tcgacctggt ttttgtgctt ggccttcttt ggcgagaacg gtctaagtat ctggattttg
     7801 ttcacaaaat gggacaatga cggtcgactt tgtgattgga tggttgcctg atgggccgac
     7861 ggtcgtacga gttcattctt cttcttcgat ccacttcatc ccgccagcgg cggcgatggc
     7921 gggggcgcag gtttcgcaaa gaacgggcaa ttcttctcgg acttcgtcgc ctcgtgtcca
     7981 gagcagcagg ccctttcccg cgggttcgcc ttcgattatt tttccgcaga cgtcgcaata
     8041 caacggttct ttgggcaggg aatcgccttt gaccgcggcg atgatcaggt gtgggcttgg
     8101 gggaacggca ttcctttgcg acgacatcga aaaggaactc tagcgcagat tgcctgagcg
     8161 ggggtacgct gctgaacgta ccgaaagccg tttctcgtag ggtcggtccg ctgcgcggat
     8221 gggccgttcg atacacaagg tcacgtttca tgcctacgct gcgaacttat ctgcccaaag
     8281 gacaacctcg acttcatagt gtcaacaaac ccatcacgac gatcggaagc tctccgagca
     8341 acgatattgt cgtgcaagcc gatggggttg cggaagcgca cgcacagatc atctttgatg
     8401 ggcgattttt caacctcgaa gaggtcgatc gtgctgccga cattcaaatc aacggaaaaa
     8461 agaagcgtcg tgccaggcta agccacaacg accgaatcca ccttggttcc gcggagatcg
     8521 cgtttagctt gctgggagat ccttcgtttg ttctcgccac agagcaggaa acctcctcta
     8581 atacaacgga catagctggt ctagagcaac tcaatgcgtt tagcgagcgt ttgatgcaac
     8641 gggggagcgt cgacgaattg ctgacggatc ttctcgatgc ggtagtggaa atcagtgggg
     8701 cccatcgtgg tgcggttttg ttggtggagc cgggagaacc ttcggatacg gagcgtcggg
     8761 tttcggttcg tgccgtacgc aatgtacgtc gcgacatcat tgcggatgcc tcgggaggta
     8821 tttctgattc gattgtgcgc aaggtcatca ccacggggat gccggtgatt gtgagcgatg
     8881 ccctggcgga ccagcagttt gggaaaagcg agagcgtcct tgctctcaag ctatcgagtg
     8941 tgatgtgtgt gccgatgctt gctcaaggcg aatctttggg agcgttgtac gtgggcaatg
     9001 atcacgtcaa acagttgttt gatcgaagtc aacttgacct tctcacggta tttgcggcgc
     9061 aagcgtcgct cattttgcag aacgccatgt tgttgtcgag cttgcgggca gacaaagaaa
     9121 agcttcagga agagctttcg gacatgcgct tcggcgagat cattggcgct tgcgattcca
     9181 tgctcgaggt gtttcgcaaa cttcaaaaag tagcgtccac ggacatcagt gtcctcatca
     9241 cgggagaaac gggaacgggc aaggaactca tcgctcggga gattcatcgt cgtagtccgc
     9301 gggtacgagg tcctttcgtg accatcaact gcggagccat ccctgaaaat ctgatcgaaa
     9361 gcgaattgtt tggtcatgtc cgcggggctt tcaccggtgc ggtggctagt cgtccgggaa
     9421 agttccaagt cgcggacaag ggaacgctat ttctcgatga aattggcgag cttcctctca
     9481 atctgcaagt caagttattg cgcgcgttgc aagagcacgt cgtgtatcgg gttggtgata
     9541 gccgccccga gaaagtcgac attcgtatcg tagctgccac caatcgtaac ttagaagaag
     9601 aaatccgcga aaatcggttc cgtgaggact tgtactatcg cttgaacgtg gtcaatcttt
     9661 ggcttccgcc gctgcgggct cggggtgagg acgtggtgtt gatagcgcgt gctcttcttt
     9721 cgaagtacgc ggatgaactc aatagctctg ttcgtggttt tgctcccgat gcgctgacgg
     9781 ccatcaaacg atatgcgtgg ccgggcaatg tgcgccaact cgaaaaccga atcaaaaagg
     9841 cgttggtgct atgcgatcac aatttgctca cggctgatga tttggatctc gttcgtgagg
     9901 agcaatcccc aattcttccg ctagagcgag cgaaagagga gtttcaacat cgttacgtac
     9961 gagaagtcct cgagcgaaac aatggcaaca ggacccagac cgcccgtgac ctgggagtcg
    10021 atccgagaac catttttcga tacctggagc gtgagcaaaa tccgattccc agtggtggag
    10081 gcaagggctg aggtgaaccg gtgttggggg acgactggtg tgggtggtgc tgctcgagat
    10141 gggagtgccg ccccgtcgtg cttcattccc atattgatag gaattgacag aggagcgtcg
    10201 cagggggagg ggattttttt agtatgatat gcgtgattat tttcgatcac gtatatcatg
    10261 aaatttatga acacaggctt attcgaggct ggatgttggt tttgggtgga gagaatcgaa
    10321 gacgtcgagg atggggttgc atcgacgggc ggtcccatcc gtcgaacgct ccaggtgaaa
    10381 ggaaagcgat tgtcaaccgt tgcttacgag agccatgaga atctggacaa gagcccccat
    10441 cctgttttct ttgacgatat cgttgttgtg tgcggttcct ttggcgcatg aggcgcgagc
    10501 ggaggtagct gcccattcgg ctatggacgc gaatgcgata gccactcgcg ttcaagcttt
    10561 ttacgacgga actcgaacgt atcaggcgcg tttcgagcag gtgtatcata tcgaggcgta
    10621 caatcgaaag aagcgttcgg cggggcaggt catctttcaa cgacccggtc ggatgagttg
    10681 gaagtacgac gagccgaatg gcaatcgcgt ggtatccgat gggcagaatc tgacgattta
    10741 cgagagggat aagtcgcagg tggtcgagca gcctcttgga acgtcgcagt atccggcggt
    10801 gctttcgttc ttggtgggaa aaggcaacct tcgcgaaatg tttcatctgc gaaagctcga
    10861 tgagaagcgg atgaatttcg aagggggatg ggttctggaa ggaacgccta aacaagccac
    10921 gtccgcgtat tcgaaagtca tcatgtacat cgacgccgag acggcgcagg tacgtcggat
    10981 gctcgtcatc gatgcccagc gcaacagaaa tttgtttacc ttttcgaatc cgacggtgaa
    11041 tacggccgtt ccgacgggtg ccttcgtgtt caagccgccg cctggaaccc aagtggttcg
    11101 gccataggtt tgtccagcat agtcgcgaac cgttccttca gtagacgttg tcacgaagga
    11161 gagcatgagc gcccgctcgg agtttcacga ccatgggtgg ggttgcgcac gatggtggtt
    11221 gtcgaaggct tggttccgtc gagccgatgg ctcgttgctt cgatgcaaga cagcccggcg
    11281 ccttgtgctg ggaaatggag aagcggatag gaggacagta gaaggtgggg cggaggggat
    11341 tgaggggccc ggggatattg cgggaggctg gttcgctggt aggtgtgttg gctgggataa
    11401 ccttggatct tactatcggc ggtggtgttg gggcggacca acgagtagcg tggtaggtgc
    11461 cgatgatcgt tggatttgga tgatctgatt gatgttccgt gggttcattg gagtagtgct
    11521 gaccgtattg gtgctgggcg tttcgttcgc ggcgagcgcc agtggatttg atttattcgg
    11581 cgttcaagcg cagggcatcg cggaggtatc ggcacgaacg gcagcggcga gggatggttc
    11641 cgcgagttat tacaatccag gggggcttgc gttggggcaa ggctacgagg ttgatttgtc
    11701 cgcgctgggg gtgatgagtc aactcgaggt tcaggatcga agttatgcca tcgacgaacc
    11761 ctggggagcg acggttacgg tggccgctgc cgttccgtta caaggccctt tggcaaatcg
    11821 aattcggctt ggtcttgcgc ttcaaggatt tggcaacacc ctgatgaagt tgaagcttcg
    11881 ggaggcatcg aggccgtttt tcccttacta cgacaatcga acgcaaaggc tcgtggtgat
    11941 tcccgcgctt tcggttcgtc tggcggaagg cgttgggctg gggctgggga tcaacacgct
    12001 agcgggaatg cagggaccga tcgacgtacg ggaaggtcaa agccggatga tggaagcacg
    12061 tgtcgtgcag gaggcgggca cggtggcgcg atggattgtt ggcgttcagg tcgaggcgat
    12121 ggaagctttg catcttggca tgacgtttcg ccagagtttt ggtgttccgc tttcgataac
    12181 gacgaccgcg gatgtggcgg ggattcctct tgtcgtcgat atatcttctg ccgaagcgct
    12241 gttcgatccg atgatggtcg tgctaggggc gaaggttgag ccttctgccg acgcatcgat
    12301 ggagttggac attgcgtatc atgggtggtc tcggtggaag gggccttggt tggggttgga
    12361 tacgactgta tctgcgctgt cgatctcggg caaactgccg gaaggccttt tccgggatac
    12421 gatgagtgta aggggcgcgg tggcgtggac ggtagaaagg gcttccgagc gaatcacttc
    12481 gctgcatggt gggcttgggt atgagacatc gatgcttgat cgcggtgagc agcaaggtcg
    12541 gaccaatctc gtggacggtg cgaaggtgat ggttggggct ggagtgacgg tgttgtttcc
    12601 gcgtgtgctg gggcagggat ttcgtgcgga aatagggatg cagatgcatc aggtcgggtt
    12661 gtatcggcag gaaaaaatcg cttgcacgag gctgccttgt ccggcgggga ccgtgttcgg
    12721 aagcgatgcc acggcaccgg atacggacat aacgaatccc ggatatccgg ttctcacggg
    12781 tggaggaagg gtgtttgtgg gaacggtggg gatgggagtc gacctatgag actgggtggg
    12841 ctcatagtcg ttggtttgtc gatgatgacg atggctcgtc ccgcggcggc gacggctggg
    12901 tatttgttcg gatgggggcc gagggatatc tcattggcgc aatccgatgt tgcggatgcg
    12961 gcgtcggtgg cggcggcttg ggtcaatcct tcgttggcgt gggagccggg ggcacgtatg
    13021 gcggtgggct atgtttacgg atggacggcg ttggagtgga atggggaacg cgtgaagttg
    13081 cgtgatatcg cgggaacgca ggttgggttt caacttgggg gcgagctggg ttcggacatt
    13141 gctttgggtg gtgcggtagt gatgcacata cccgatcgat cgttggcgcg ggtatccttt
    13201 ttgcccggaa cggagcccgt ctttttccga ttcgatcctg cttcacagcg cacgactatg
    13261 gatgcggtat tggcattgcg tttgggttgg ttgtcgatcg gtgtgggagg ggaagtgttt
    13321 gtatcagcgc aaggcgatgt ggatttcgtg ttgggacaag atgccaacgg cacgtatgcg
    13381 gatggtgagg cgcaggtaac gttggcgtac gatgtgacgt ggctggcggg actgacgatg
    13441 gacttggggg cggcgaagct cggtttgcgc tatcgaggcg cgcaagcgtt ggatgtggat
    13501 ttgtcgacgc ttgccacggt ggatgtgcag ggcaatcctc tcaacggtac gacgaaagtg
    13561 cggatagttg ggcgaagtgg ttatgtacct gcgacggtgg atttgggagc gagttggacg
    13621 gtgggggcgg tggggcatgt gatgacatcg ttgcagtacg cgcggtggag tgatgctccc
    13681 agtccagtgg cggccttgtc gatggatgtt gatttggggt tatctccggg gttgcggcaa
    13741 ggctcgttcg tgatgcctgg ctttcgggat acggtatctc cacgggtagg gatggagatg
    13801 gaaccttggg gagaagaggc ggtagctttg cgtgcgggtt atcagtactc gccctctccg
    13861 gttcgcgttt ctcgcggctt tgcatctccg atggacgcgt cgacgcatgg gtttgcgtta
    13921 ggggcagggg tggattgcgg aagggtgtgg ggggtcgata ttcgttcgga tgcggcatgg
    13981 caatggttga tgctcgagcg acggactctc gacaaacctg gagatgcgct tccgttttcg
    14041 cattccgagg tgagcgggtc ggtgaagctg atgagtgtat cggtacaagg ggggtggcta
    14101 tgaagcgttg ggtaggtatg ctcttcgtag gcttatcgct tgcttgtgga ctactcgtac
    14161 ctgaggatct gaccgaaagc gcgcgtcgtg atgaatacag cgggtttgtg gagggtgggg
    14221 ccattcaggt gtgtttcgat ggggtgccgc tgatatcacc cgcgttggcg aaagagggac
    14281 cgttatcgat ttgcatggac aggcaagggg atgagcgtcg ttgtggacct ggtgcggggt
    14341 gcggcagcgg ggagcgctgt atttgtggtt tgtgttcgac gaggccttgt cggagcgcga
    14401 cggagtgtga agggggcgag gtgtgccaaa gcaatcgttg tacgaagtcg tgtggagaca
    14461 atcgcaagtg tggtgaaggg gagagatgtg aagcgggagc ttgtgttcgc ccgtgcatgt
    14521 cgcatggtca atgtgctcat ggggaacggt gcagcacgtt cgatggtacg tgtgtggtga
    14581 agttatgtgg tgagcaagtg ggctgtgggg ggagtgaggt ttgcgtggcg cggcaagtgg
    14641 tggctgacgt gcgggagccg actgtggtgt ctttcgacga agggaaggta gcgtatgtcg
    14701 aagtgcgtgg aggaccagcg tatccgaatg cttgcgcggt ctatcgtgta cgtgtgcaag
    14761 agatgaaccg ttgggtcatc gagccatcga cgccggtatt ggtgccgcgg gaaggtgatg
    14821 ggggatgcct tgggggacct tcggtgctgc gagggggagg cggcgtcgtg atggtggcgt
    14881 cgcggggaga tggcagtggc attgtgaagg cgacatcgcg ggatggggta aatttcgata
    14941 gtgacgcagg ggtgatgttg gagccggcgt tgcagtggga gcgggggtgg gtgggggcac
    15001 cggccttggt ggcgtggcgg ggcaagtggt tgatgttgta cgaggcagga cgaggagcag
    15061 gaattgggat tgccaacgtt agtgagtccg gagtggtacg ccattcgaac gaaccttggt
    15121 tggtgccttc gcatttcgag gatccgtcgg tatggcggga tgtgcaacgt attggttcgc
    15181 cgtatgggat cgagcgagag ggcaagcttt gggtatactt gacggtgcgg ggagtcgagg
    15241 gattggatgc ggtgtcacgg caggggcagc gatac
//
LOCUS       DAOSOR010000058        15210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_3928_length_15210_cov_4.230023, whole genome shotgun sequence.
ACCESSION   DAOSOR010000058 DAOSOR010000000
VERSION     DAOSOR010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 15210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15210
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3928_length_15210_cov_4.230023"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..1515
                     /locus_tag="PK710_04925"
     CDS             220..1515
                     /locus_tag="PK710_04925"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT09090.1"
                     /translation="MSKGCRAWVIGIAAVLLPGCPDKPGVEHSAVASAGSGPSVALTS
                     SSAQAVGSTAPTVVASNAVVEAPTLPPLPIPPDKPSAMPSLADFRKAPTVEIGYATAL
                     GCEARMLRDWIMVQCKEGPATDKPVGANAIRTQGTDARVRVSKVGDGVQVTMPLRKGV
                     EAKAEILWRTPGWGKRLVRVVYETTDERPVVTFDAPPPGPEQSPLSLGQLPGEKPHVG
                     RMILVPAGARKGKEPVEAFYLDETEVTFHAWNACVQDGACPPPSGATGCPRSTQGVSP
                     LRPINCITWNEARAYCAWAGKRLPSEQEFWFAYYGSDDRIYPWGNAVEIERICDYQQH
                     EWVEDAGKRRLLNLGCKVGSHPLGKGPFSHEDLLSNVAEWTSDEHDGKKVVLGGAFGS
                     VSGMEWRDYWGWLMKDLRKTMEPDSRQTEIGFRCARDAH"
     gene            1752..2882
                     /locus_tag="PK710_04930"
     CDS             1752..2882
                     /locus_tag="PK710_04930"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOT09091.1"
                     /translation="MAKKTKIQCIGILTAGGDCPGLNAAIRGVAKAAIAAHDIKVIGV
                     LDGFRGLVENRTVMLDNRSVSGILTQGGTILGTSRDKPQRMSMGGKIMDMTTTAVENA
                     RRLGIDCLVCLGGGGTQKNALHLHQNGGMNVITLPKTIDNDVYGTDVSFGFDTAMGIA
                     TEAIDRLHTTATSHNRLIVCEVMGHKAGWLALGAGVAGGADVILIPEIPYDLNVVAEH
                     LLRRRREGKRFSIIVVAEAAQARANETEADAKQEKKRNGKEGDSVAPSPAINLVQESM
                     ASRLARQLQQLTETEARVTSLGHVQRGGIPSATDRLLCTRLGVHAAHLLGEGTYNVMV
                     AVRDGDCRAVPLEEVAGKKKTVPMDHPWISAARDVGTCFGDA"
     gene            2939..3592
                     /locus_tag="PK710_04935"
     CDS             2939..3592
                     /locus_tag="PK710_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09092.1"
                     /translation="MGNWSGKKKPKNKLKRKAKAEKRLERKIEEARLSESGDATDSRA
                     SGPSDGSAAAQTPAQKNWLERIRGVWNGFSPQARVGTLLGVAAILAVVAYQYIETRPT
                     GPTAASASSVTAPAATTLPPVSWQGLPALSASQGTIIAVPPTMASSQASAVSSGSTPP
                     PAAPAASVQPSAAPSPEPSPSTSAPAAPKAPPPKPAAPKAPPAVPPPAPPADNNNPY"
     gene            3813..5024
                     /locus_tag="PK710_04940"
     CDS             3813..5024
                     /locus_tag="PK710_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HOT09093.1"
                     /translation="MAFDPARFPVWLLFSLLGCVTPGLSRGPETSGAKSTAPVSSSSG
                     PQPPRTEAAGPWESEALEDDDDDPILDDGGGDDDEASDDSKDSVAAYRPVDHPLASIS
                     DAELERMVQTDLASLGPMSLGATNAGRLINAVQMPKGERWELIDPARAWGTQETVDGI
                     IRCIDEVHARFPDSPKMQIGHISARQGGALSPHVSHQAGRDVDLSYYYTSPTRWFTRA
                     HAGNLDRARTWAFVRALIVHTDVEMILIDGGLQRLLREHALAIGEDPAWINDIFRGSQ
                     GRPALIRHARGHANHLHVRFYSPVARETARRVQPLLAKTREMDPPVAYVMHRARKGDT
                     LGRLANHYGTTVEAIQRANGLRSTAIRAKRTYRIPTRGRVVPVVPQPISVPPRRLPPE
                     KQALRTTGDNP"
     gene            5114..6487
                     /locus_tag="PK710_04945"
     CDS             5114..6487
                     /locus_tag="PK710_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOT09094.1"
                     /translation="MKRWSWFLGAVAVGLFIGCGTSSDSSPDGSGGSAKGGSGGSGAT
                     GGKGGSSGDASVDGGSGGESGSAGVSGGWPTGGSGGGGTGGSSETGGVGGSTQPDASP
                     DVNFEYDAPVTEASLEDACVDETAAAKPVPLDIYFMLDASTSMSIPAGSGAQGDCNVA
                     PPFSPSVNSRWCNAVNAVAGYVSDSSVAGNRAAIGYFKHYTSHNCNGSGYDTPMVALG
                     TLSGGYTGHAKVIVEQAYGGLNWAYPFSNTPTGGALNGLVKFTAANKTPGRVIIGILI
                     TDGAPNECESSDSGLRNIAQTHFNNTGIHTFMVGIEGSAFQRLEAWASYTGAIKHDDT
                     NDACGNCNNCECHHYNVGKGNPAVFIEALKKIQKSVLSCTFQVPEPSQGVLDPDLVRV
                     DYFPNGQPPPIELPRVNSAGDCSGPGWYYDNPTNPTTVNLCPDSCSTIQADSHAEIKI
                     RIACQAS"
     gene            complement(6488..7909)
                     /locus_tag="PK710_04950"
     CDS             complement(6488..7909)
                     /locus_tag="PK710_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOT09095.1"
                     /translation="MTSKVDASSLVQTTSVTQTNAKVNDIVSRASRWAARGRLEEALQ
                     LLRQAAFDHPNHSTVHEWLGLVALQDARLDMAAAFLQRAIALDPKRASAYASLSDVFA
                     AKGQRSEAENALRSALRIAPSLGSAWLRLAHLRRFRKKDDPDFRRLRDLCRNHGPDTT
                     DREALYFALAKAYDDLGCPNEAFRFMKTANEMHRERHPFDIQAMQQLMDRVSTVCDKE
                     LFSKYNTLGNDSSLPVFLVGLPRSGSSLVERIIATHPQGDGVGEIGTLPRLAADLPSR
                     VGTQTPFPECLREMSGEAIQAVAGEYLRRLTRDASATTLRICDKMLSNLVLVGLIAIL
                     FPRARIVYCNRNILDAGLSMYQQSFQGGGVGYTYDLEDIGKVYRMSSQLMEHWKRVCP
                     IAIEQVQYEALVRDPETQVRRLLDSLGLPWDPVCLSHHRGGSTQTVSNWQVRQPTHTR
                     SVERWRKYSKHLTPLRKVVERSM"
     gene            complement(7906..9135)
                     /locus_tag="PK710_04955"
     CDS             complement(7906..9135)
                     /locus_tag="PK710_04955"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOT09096.1"
                     /translation="MAIIVQKYGGSSVADVGKIKRVADKIVDTFHAGNQVVVVVSAMA
                     KATDSLLELAYQAAAVDGKSSAPARRELDMLVTTGERVSMALLSLAIQARGCDAISFT
                     GSQCGILTNDRHFDARIIEVRPHRVEDELARGKIVIVAGYQGVSYKREITTLGRGGSD
                     TTAVALAAALGADRCEIYSDVDGVYSADPRAVPNAKHIPELNPELLQEMAESGAKVLN
                     AQAVEWARKNGIAIYARSTFDPCTPDGQPRRETIVRQFAPQNDNRVRAITCEKNIALI
                     RLSTNDTLSFRAATAHLRQFDIPVRELSLSDHGGSFLIPLNNVPEWEQIQPRLIRDTP
                     WKQATIDNEVAVVSVVGHGLTESAEPMARMVEALEKTNVPLLELRGSSLRLGATVAAN
                     HAITAQQALHRAFVEES"
     gene            complement(9187..10662)
                     /locus_tag="PK710_04960"
     CDS             complement(9187..10662)
                     /locus_tag="PK710_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09097.1"
                     /translation="MQAAAQSNDDLLDDLPPLDGEEEGEEQDVGLQQEFLEENDTACG
                     EEQDGFPDFSLPVGELIDTPWDIDRPSLEEGDELAGVDPVQDDIPPFDISHRREDDDD
                     EGVTNDDIALGIDDLEAVERDDGAEGTSDSLDNEVNESAFPHLDTSDDGDDGDQVDVG
                     LIDLPDEVALPAWAEARWHRTHTPLVAAPMSFVICTPRLVAAAGHGICFLDECEGTER
                     CAVLPIPDSKHSEFVSMGFAPRDRVMLASRQRFFETTRSVQTCQWFESGLSKGDAIAH
                     VVYGLCDRAFSYASTTQGELLVSADHGRTWKRVDGLGKVLGLTSDEKGNAHILTVSET
                     SFFWLDADDEGRWESKLVKAVLSTHGVAALAASSDTWVAALEDGALLASPDRGETWFH
                     TKIPVGIQSLAILPNEAKATIVGAIYLESHDRSVLFSWTPGQEPTLVADLSPDVAVVG
                     ADADPTEGMGRAHHLRWDPFRGCLWVAGAFGLIAWSPSLPT"
     gene            10676..11110
                     /locus_tag="PK710_04965"
     CDS             10676..11110
                     /locus_tag="PK710_04965"
                     /inference="COORDINATES: protein motif:HMM:NF027581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4911 domain-containing protein"
                     /protein_id="HOT09098.1"
                     /translation="MQQCRKGISQVVFHDSRDGDGGVSKPCHGTVLQKRRGSDREPVS
                     EQSERVEEAMSVPIPTVDDDALVWYRIRVVEKDVAFVRGILEASEGLACMFAEKGGDL
                     MLVSPSSQQQRLEEVIEDLRVELGAIVTERGTNGGRKRESSS"
     gene            11163..11849
                     /locus_tag="PK710_04970"
     CDS             11163..11849
                     /locus_tag="PK710_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09099.1"
                     /translation="MSSTPSREAINDADVSRSRVEGAWRVWLEALATDAEAALGASLA
                     YEALDEGGRNAVLDILEKDVATLAVPRIAVFAPLLSVERDSQRRDRIRIAIGSDMDER
                     RQISARALMGTRSDGDQIIVAETHAYLDFVRLTVCRLDPDHGIRWVRSEPLVHARDAT
                     KPGTRMYEADLEPLPVRTAVDIIARAIVAQRRRDGVLPPSLLPLLELFDVGTEDPLGG
                     KDGYVTEGKG"
     gene            11857..12420
                     /locus_tag="PK710_04975"
     CDS             11857..12420
                     /locus_tag="PK710_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HOT09100.1"
                     /translation="MRHRPFRGLLAILFALWGCGGAAIPPAHDESEEGSHAVRLLPSG
                     DTPRFRFRALDGREVSSDVALGRTSVIAFMATFDWASQAQARFISGIERHHTPRVNCF
                     GIVMEQAENAPLVESFVTTLGLRFPVAHVTSSQVGKATLGVRTVPTVLVMDAGGNVIW
                     RSRGLATEDQLREVLHAVEAQTKKKVK"
     gene            12489..12806
                     /locus_tag="PK710_04980"
     CDS             12489..12806
                     /locus_tag="PK710_04980"
                     /inference="COORDINATES: protein motif:HMM:NF021188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HOT09101.1"
                     /translation="MKHAVAHDLSIPLAKAAVNKAFESYCARFAEYKPTLRWVSDRKA
                     DAGFSVKGFSLKGGFEVTDSTIELELDVPFVLRIFKNQALSVIEKEVLLWVSKAKAGE
                     ITV"
     gene            complement(13020..13592)
                     /locus_tag="PK710_04985"
     CDS             complement(13020..13592)
                     /locus_tag="PK710_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09102.1"
                     /translation="MTQPRPNKPPTKTVPLRTTKSSDPDQIPVDGERFEILWLQFEDE
                     AGFSTGRLDDERAKLQQEIAVLEAKIAEKKSCLQMVENQVDAAKQQLKVLLSAKLSDD
                     AILTAMRVEYKIKRSAPKPKKDDVADSPADEDKQFVLDQLDAEGLSISDLRKLTGKDA
                     RTLRITLEALVAEGKISKTGDRANAKYHLV"
     gene            complement(13619..14527)
                     /locus_tag="PK710_04990"
     CDS             complement(13619..14527)
                     /locus_tag="PK710_04990"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOT09103.1"
                     /translation="MTQREDAAPNIAIFADFENVALGVRDANVEPFNISLVLERLLDK
                     GKIVVKKAYADWDRYKSHKRSMHEAAFELVEIPHISYSGKNSADIRLVVDALDLCYTK
                     THIDIFVIISGDSDFSPLVSKLRENDKKVIGLGVKNSSSDLLVGNCDEFIYYDDLIRS
                     LRTGIKPTAKRDANKGKAPAKPPAKATRSEGQPKPELVKPDSTNASSPAVDMDTKSVD
                     ERREEAIEMVLDVVESLFRDRDDNLWGSMVKQTLKRKKPNFSESFHGYRTFNELIEDA
                     QRRGLLETQKDERSGGYLILSFGPNA"
     gene            14689..>15210
                     /locus_tag="PK710_04995"
     CDS             14689..>15210
                     /locus_tag="PK710_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09104.1"
                     /translation="MVRKQPNRLTWVVGSFFVLVGACSIVAEVDRENIPSNETPSTGG
                     SGGTGGAGGAGGTGGVGATGGSGGAGGCTVPSDCPGTDTDCASRTCVGNSCGVEYAAA
                     GTLTSQQVEGDCQSLVCDGSGSTIKVNDDKDIPAQDNECAVGSCVAGAPKVDFAPEGT
                     ECSSNGGNVCDGEG"
BASE COUNT         3203 a         4057 c         4598 g         3352 t
ORIGIN      
        1 ttatcgcgct ggactgccac tcaacgaatt gaaggggcgc gaaacctacg ttgaaggcaa
       61 tgtcaatagc tgtgtcgtga cgcgttcgac caacggttcc aacgtggagt attatggctc
      121 gttggaggga atgttctgac cgaacggtca tgagtcgacc gagcctattc agcccaattg
      181 ccacgtgacg acgccgggga tacgtgcgag catcaagcca tgagcaaggg gtgtcgagcc
      241 tgggtgattg gcatcgcagc ggttctgttg ccgggttgtc cggacaaacc tggcgtcgag
      301 cattcggcgg tcgcttccgc gggatcgggg ccctctgtgg ccttgacgtc ttcttcggcg
      361 caagccgtcg gttcgaccgc tccgacggtg gtcgcatcca acgcggttgt ggaggcgccc
      421 acgctgccgc ccttgcccat tcccccggac aagccttccg cgatgccatc ccttgcggat
      481 tttcgcaaag ctccgaccgt tgaaatcgga tatgccacgg ctcttggctg cgaggcgcgc
      541 atgcttcgcg attggatcat ggttcagtgc aaggaaggac ctgccacgga caaaccggta
      601 ggagccaatg ccatccgaac gcaaggaacc gatgcgcggg tgcgggtgag caaggtcgga
      661 gatggggtac aggtgacgat gcccttgcgc aagggggtgg aagccaaggc ggagatattg
      721 tggcgaacgc cggggtgggg caagcgattg gttcgagtcg tctatgaaac caccgatgag
      781 cgtccggtgg tgacgttcga tgcgccgccg cctgggccgg agcaaagccc gctttccctc
      841 ggtcagcttc cgggcgagaa acctcatgtg gggcgaatga ttctggttcc cgcgggggct
      901 cgcaaaggaa aggagcccgt ggaggctttt tatttggatg aaacggaagt gacctttcat
      961 gcatggaatg cttgtgtgca agacggagca tgcccgcctc cgtcaggggc aacggggtgc
     1021 ccgcgctcca cgcaaggggt gtcaccgcta cgtcccatca attgtatcac ttggaatgag
     1081 gctcgcgcgt attgtgcctg ggcaggaaag cgtcttcctt cagaacaaga gttctggttt
     1141 gcctattatg gttccgacga tcggatctat ccgtggggga atgcggtcga gatcgagcgg
     1201 atctgcgact atcaacagca cgaatgggtg gaggatgcag gcaaacgtag acttctgaac
     1261 ctggggtgca aggtgggctc gcatccatta ggtaaggggc ctttttccca tgaggatctg
     1321 ctatcgaacg tggccgaatg gacttccgac gaacacgatg gaaaaaaagt cgtgctcggc
     1381 ggagcctttg gttccgtcag cggtatggaa tggcgtgact attggggttg gttgatgaag
     1441 gacttacgca aaaccatgga gccggattcg cgccagacgg agatcggctt tcgatgtgct
     1501 cgggatgccc attgaaggag gtttccctca tcgaacatcg gctcgtctcc ctccatcggc
     1561 tatccatata cggatagagc gaagagggct gtcgattcca ggtccatcgc gtggagcggg
     1621 acccaaagcg aagaggccga gatgtatgct ctcgcgggtg aatcgccatt tgtccgtgga
     1681 gtcatggatt tttcgctgga gatcatcgtt cgaggtgctt acgattcctg gcgacgaaac
     1741 ggagcaaggg cttggcgaag aagacgaaaa ttcagtgtat tggaattctt acggcgggag
     1801 gggattgtcc gggattgaat gcggccatac gaggggtcgc gaaggctgcc attgccgcgc
     1861 acgacatcaa ggtcatcggt gtgttggatg gctttcgtgg ccttgtcgag aatcgcaccg
     1921 tgatgttgga caatcgttcc gtatcgggca ttctcacgca agggggcacc attctcggca
     1981 cgagtcgtga caaaccccag cgcatgtcga tgggcggaaa aatcatggat atgaccacca
     2041 cggccgtcga aaatgctcgc cgtctgggca tcgattgtct ggtttgtctc ggagggggag
     2101 gaacgcaaaa aaatgcgttg caccttcacc aaaacggcgg aatgaacgtc atcaccttac
     2161 ccaaaaccat cgacaacgac gtgtatggta ccgacgtatc ctttggtttc gatacggcca
     2221 tggggattgc tacggaagcc atcgatcggc ttcacaccac cgccaccagc cataatcgtc
     2281 tgattgtatg tgaagtgatg gggcataaag caggatggct tgcgcttgga gctggcgtag
     2341 ccggcggagc ggacgtcatt ttgatccctg aaatccctta tgacttgaac gtggtggcgg
     2401 aacatctgct gcgacgtcgt cgggaaggca aacgtttttc catcatcgtt gtggccgagg
     2461 cagcccaggc gagggcgaac gagacggaag ccgacgccaa gcaagaaaag aaacgcaacg
     2521 gcaaagaggg tgattccgtg gctccgagcc cggccatcaa cctggttcag gagtccatgg
     2581 catcgaggct tgcgcggcag ctccaacagc ttacggaaac cgaggcgcgg gttacctccc
     2641 tggggcatgt gcagcgcgga ggaattccga gcgcgaccga tcgtttgctt tgcacccggc
     2701 ttggcgttca tgcagcccac ctgcttggtg agggaacgta taacgtgatg gtggcggtaa
     2761 gggatggcga ttgccgcgcg gtgcccttgg aagaagtggc tggtaagaaa aagactgtgc
     2821 ccatggacca cccatggatt tccgcggcga gagacgtggg cacgtgtttt ggcgatgcgt
     2881 aagcgatcgg tgcgcgtttg cccttggttt ggctaaaaaa aaccgatatg gggtcgccat
     2941 gggtaattgg tctggaaaga agaagccgaa aaacaagctc aagcgaaaag ccaaagcgga
     3001 aaagcgcctc gagcgaaaaa tcgaagaggc aagactgtcc gaatccggcg atgccaccga
     3061 ctcccgggca tccggaccgt ccgatggcag cgccgccgct caaacaccag cacaaaaaaa
     3121 ctggctggag cgcatccgtg gggtctggaa cggcttttcc ccccaggctc gcgtgggtac
     3181 cctgctggga gtagccgcta ttttggcagt ggtggcctat cagtacatcg aaacccgtcc
     3241 caccggcccc actgctgcat ccgcctcctc ggtgaccgca cctgcggcaa ccacgttgcc
     3301 ccccgtttct tggcagggtc ttccggcatt gtctgcaagc caaggcacca tcatcgctgt
     3361 cccccccacc atggcttcct cacaagcgtc ggcggtcagc tcagggtcta caccgccccc
     3421 tgccgccccg gcagcgtcgg tgcagccatc ggctgcgcct tctcccgaac cttccccatc
     3481 cacctctgcc cccgccgctc cgaaagcgcc tcccccaaag ccagcggctc ccaaagcccc
     3541 tccagccgtc cctccccctg ctccccccgc cgacaataac aacccctatt gatctttccg
     3601 ccccatcgac ggccaccata ccccccgaaa accatggttt cgtcttctct aacctattgt
     3661 aatatataaa tatttcgtga tttctcgcca gctttcgacg ttgtcgccgc cctcgaacga
     3721 acccatcctg tcgcgcgtcg ccggcttgtg cgcttaccaa aaatcctgag caagtggcgc
     3781 gcgcttcgat cgtccgctac gttgacgttt ccatggcttt cgatccggcg aggttccccg
     3841 tttggctcct attttcgctc ttgggctgtg tgacgccggg cctgtcgcgc ggacccgaaa
     3901 cgagcggagc caagtcgacc gcgcctgttt catcgtcttc tggcccgcag ccacctcgaa
     3961 cagaggccgc cggaccatgg gaatcggaag cgctcgaaga tgacgatgac gatcccattc
     4021 tcgacgatgg tggtggtgat gacgacgaag cttcggatga ttccaaggat agcgtggctg
     4081 cctatcgtcc ggtggatcat ccgctggcat ccatctccga tgccgagttg gagcggatgg
     4141 tgcagaccga cttggcatcg ttaggaccga tgtctttggg agccacgaac gcggggcgtc
     4201 tgatcaacgc agtacaaatg ccgaaagggg agcggtggga gttgatcgat ccggcacggg
     4261 cctgggggac gcaggagacg gtcgatggga tcattcggtg catcgatgag gtgcatgcgc
     4321 ggtttccgga ttcgcccaaa atgcaaatcg ggcatatcag cgcgcgtcag gggggggctt
     4381 tgtcccctca cgtgagtcat caagctgggc gtgatgtgga cttgagttat tattacacgt
     4441 cgccgactcg ttggtttacc agggctcatg cgggcaatct ggatcgagcc agaacatggg
     4501 ctttcgtgcg ggcgttgatc gttcatacgg atgtggaaat gatcctgatc gacgggggat
     4561 tgcagcgatt gttgcgagag catgccttgg cgatcggtga ggatccggct tggatcaacg
     4621 atatttttcg aggatcccag ggacgtccgg cgttgattcg tcatgcgcgc ggccatgcga
     4681 atcaccttca cgtgaggttt tatagtccgg tggctcggga aacggctcgg cgtgtccaac
     4741 ctttgttggc gaaaacacgc gaaatggatc ctcctgtcgc ttatgtgatg catcgggcgc
     4801 gcaaaggaga caccctcggt cgactggcca atcattatgg aacgacggtg gaggcgattc
     4861 agcgagccaa tggtctgaga tcgactgcca ttcgtgcgaa gcggacgtat cggattccta
     4921 cgagggggcg ggtcgtaccg gtggttcctc aacccatttc ggtgccgcca cggcgtttac
     4981 cccctgaaaa acaagctctc cgaaccacgg gcgacaaccc ctaaaaaggg atgagccaag
     5041 cttttggtgc attatcgctc ccgcgcacat ctaccgagtt gcattgcact cgttcgcatg
     5101 cgacactcga aacatgaaac gatggtcgtg gtttctaggc gcggtagccg ttggcttatt
     5161 cattggatgc ggtacgagta gcgattcgtc gccggatggg tcaggcggtt ctgcaaaggg
     5221 aggttctggc gggagtggag ccacgggcgg aaaaggtggt tccagtgggg acgcaagcgt
     5281 cgatggtggc tctggggggg agagtggatc ggcaggagta tcgggcggtt ggccgacggg
     5341 cgggtcgggc ggaggtggaa cgggtggctc cagtgaaacc ggtggagtcg gtggaagcac
     5401 gcagcccgat gcttcccccg acgtcaactt cgaatatgac gctccggtga cggaagccag
     5461 tttggaagat gcttgtgtgg atgaaaccgc ggcggcaaaa cctgtcccgc tggacatcta
     5521 cttcatgttg gatgcttcca cgagcatgtc gattcccgcg ggttccggtg ctcaaggtga
     5581 ctgcaacgtg gcgccgcctt tttccccttc ggtcaattcc cgctggtgta atgcggtgaa
     5641 tgcggtggct ggctacgtct ccgactcgtc ggtggctgga aatcgtgcgg cgattggcta
     5701 cttcaaacat tacacaagtc ataattgcaa tggttccgga tatgacacgc ccatggttgc
     5761 gctcggaacc ctgtccggcg gctacaccgg ccacgcaaag gtcattgtcg aacaagcgta
     5821 tggagggctg aattgggcct atccattcag caatacacca acgggtggag ccctgaatgg
     5881 cctggtgaag ttcaccgcgg ccaacaaaac tcccggacgt gtcatcatcg gcattttgat
     5941 taccgatggt gctcccaacg aatgtgagtc aagcgatagt ggcttgagaa acatcgctca
     6001 aactcacttc aataacacgg ggatccacac gttcatggtg ggcatcgaag gttcggcctt
     6061 ccaaaggttg gaggcttggg caagttatac gggcgccatc aagcacgatg atacgaacga
     6121 tgcttgtggc aactgcaaca attgcgaatg tcaccactat aacgtcggca agggcaatcc
     6181 tgccgtattt atcgaggcct tgaagaagat ccagaagtcc gtgttgtcgt gtactttcca
     6241 ggtgcccgag ccgagccaag gagtcctcga tccggatctc gttcgtgtcg attacttccc
     6301 caacggccaa cccccgccga ttgaattgcc tcgcgtgaat tccgcgggtg attgctccgg
     6361 acccggctgg tattacgata accccacgaa tcctaccacc gtcaatctct gtccggattc
     6421 ctgctcgacg attcaggcgg atagtcacgc ggaaatcaaa atccgtatcg cctgtcaggc
     6481 ctcctgatca catggaccgc tctaccacct ttcgcagggg cgtgaggtgc ttgctgtatt
     6541 ttcgccagcg ctctaccgac cgtgtgtgcg tgggctggcg cacttgccag ttgcttaccg
     6601 tttgtgtgct gccaccacga tgatgcgaaa gacaaaccgg atcccagggc aatcccaggc
     6661 tgtcgagtag tctgcgaacc tgcgtttccg gatcgcggac caatgcttca tactgcacct
     6721 gctcaatcgc gatggggcaa acccgtttcc aatgttccat gagttggctg gacattcgat
     6781 acaccttgcc gatgtcttcc aaatcgtagg tgtagccaac tccgccgccc tggaacgatt
     6841 gttgatacat cgacaggccg gcatccagga tgttccggtt gcagtacaca atccgtgcgc
     6901 ggggaaaaag aatcgcgata agcccgacca acacgagatt cgatagcatt ttgtcgcaga
     6961 ttcgaagagt cgtggcagaa gcgtcccgcg tgagtcgtcg aagatactcg ccggcaaccg
     7021 cctgaatggc ctcgccactc atctcgcgca agcattccgg aaaaggcgtt tgcgtcccta
     7081 cacggctggg caaatccgcg gccaggcgtg ggagcgttcc gatttctcca accccgtcac
     7141 cttggggatg cgtggcgatg atccgttcga ccagactcga accgctgcga ggcaggccca
     7201 cgagaaaaac cggaagggag gaatcattgc ctaaggtatt gtatttgcta aataattcct
     7261 tgtcgcaaac cgtgctgacg cggtccatga gttgctgcat ggcttggata tcgaaaggat
     7321 gacgctcgcg gtgcatttcg ttcgcggttt tcatgaagcg gaaagcttca ttggggcaac
     7381 ccaggtcatc gtaggctttc gcgagcgcaa aatagagggc ttcgcgatcg gtggtatctg
     7441 gcccatggtt gcggcaaaga tctcgaagcc gacgaaagtc aggatcgtct ttttttcgga
     7501 aacgtcgaag atgagcgagg cgtagccacg cggaacccaa cgaaggagcg attcgaagag
     7561 ccgagcgcaa tgcgttttcc gcttcggaac gctggccttt ggcggcgaat acatcggata
     7621 aggaggcgta cgcggaggcg cgtttcggat ccagcgcgat cgctcgctga aggaaggccg
     7681 cggccatatc cagacgtgca tcttgcaaag ccaccagccc gagccattca tggacggtcg
     7741 aatgattggg atgatcgaat gctgcttggc gaagcaactg cagtgcttct tccagacgtc
     7801 ctcgggcagc ccaccgggac gcgcgagata ctatgtcatt caccttcgcg ttcgtttgcg
     7861 taacgctcgt ggtttgcacg agagaagagg catcgacttt cgatgtcacg attcttccac
     7921 gaatgcccga tgaagcgcct gttgggcggt gatggcatgg tttgccgcga cggtggcacc
     7981 aagacgaagg gaggaaccgc gcagttcgag gagagggacg ttggtctttt ccagcgcttc
     8041 taccatgcgc gccatcggtt ccgcgctttc ggtgagcccg tgtccgacca cgctcacgac
     8101 ggcgacctca ttgtcgatgg tagcttgttt ccatggtgta tcgcgaatca ggcggggctg
     8161 gatttgttcc cactccggga cattgttgag cgggatcaga aatgaaccgc catggtctga
     8221 gaggctgagt tctcgaacag gaatatcgaa ttgtcgaagg tgggccgtag ccgcgcgaaa
     8281 cgacagcgta tcgttggtgc tcaagcgaat gagggcgatg tttttttcgc aggttatggc
     8341 acgcacgcga ttgtcatttt gcggagcgaa ttgtcgcacg atcgtctcgc gccgcggctg
     8401 accgtcgggc gtacaaggat cgaaggtgga tcgagcatag atggcgatgc cattttttcg
     8461 ggcccattct acggcttggg cattgaggac tttggcaccg ctttcggcca tttcttgaag
     8521 caattccgga ttcaactccg ggatgtgctt ggcattggga accgcccggg gatccgcgga
     8581 atacacacca tccacatcgc tatagatttc gcaccgatct gcgccgaggg cagccgccag
     8641 ggcgaccgcg gtcgtatccg atccgccacg tcccaacgtg gtgatttctc gcttgtaact
     8701 taccccctgg tagcccgcga cgatcacgat ttttcccctc gcgagttcgt cttcgacacg
     8761 gtggggacgt acctcgatga tgcgcgcatc gaagtgtcga tcgttggtca ggatgccaca
     8821 ttgggatccc gtaaaagaga tggcatcgca gccccttgcc tggatggcga gagataaaag
     8881 agccatggaa acgcgctcgc ccgtggttac gagcatgtcg agttcccgtc gcgccggcgc
     8941 cgatgacttg ccatccaccg cggccgcttg atacgcgagt tccagcagcg aatcggtggc
     9001 tttggccatg gcgctgacga cgaccaccac ctggtttccc gcgtggaagg tatctacgat
     9061 tttatcggca acacgcttga ttttaccgac atcagcgaca gacgagccgc cgtacttctg
     9121 aacgatgatc gccatggcaa tgaggtagca ctgatgctgg gggggtggga acggtgaatg
     9181 attggttcag gtgggaaggg aaggactcca ggcgattaaa ccgaaggcgc ctgccaccca
     9241 aagacagcct cggaatggat cccaacgcaa atgatgcgct cgtcccatgc cttccgtggg
     9301 atctgcatcc gccccgacaa ccgctacgtc gggcgataag tcagcgacca acgtgggctc
     9361 ttgtccgggg gtccaagaaa acagcacgct cctatcgtgg gattcgagat aaatggcacc
     9421 gacgatcgtt gccttcgctt cgttcggtaa gatggcaagg ctttgaattc caacaggaat
     9481 ttttgtgtgg aaccatgtct caccgcggtc cggcgatgcg agcaatgcgc catcttcgag
     9541 cgcggcaacc caagtgtcgg atgacgcggc caatgcggcg acgccatggg tcgaaaggac
     9601 tgctttcacg agcttcgatt cccatcgccc ttcgtcgtcc gcatccagcc agaaaaagct
     9661 tgtctccgaa accgtgagga tatgagcgtt tcctttttcg tccgaagtca gtccaagcac
     9721 cttccccaaa ccatccacgc gtttccacgt ccgtccatgg tcagcgctga cgagcagttc
     9781 gccttgggtg gtgctggcat aggaaaatgc cctatcgcaa aggccataaa cgacgtgcgc
     9841 tatggcatct cctttcgata atccgctttc gaaccattga caggtctgca ccgaacgagt
     9901 cgtttcaaaa aaccgctgac ggctcgcgag catcacccgg tctcgcggag cgaagcccat
     9961 cgacacgaat tcactgtgct tactgtctgg aatgggcaat acggcacagc gttccgttcc
    10021 ttcgcattca tcgagaaaac aaattccatg gcccgcggcg gcaacgagtc gtggagtaca
    10081 tatcacgaaa gacatgggtg cggcgacgag tggtgtatgc gtacgatgcc aacgagcctc
    10141 cgcccacgcg ggtaaagcga cttcatcggg cagatcgatt agcccaacgt ccacttggtc
    10201 cccatcatca ccgtcgtcgc tcgtgtcgag gtgcggaaaa gcggattcat tgacttcatt
    10261 gtccaacgaa tccgaagtac cctccgcgcc atcatcgcgc tcgacagcct cgagatcgtc
    10321 gattccgagt gcgatatcgt cgttcgtaac tccctcatcg tcatcatcct cacgccgatg
    10381 tgagatgtcg aatggtggga tgtcatcctg caccggatcg accccggcta gttcatcacc
    10441 ttcttcgaga gaagggcgat cgatatccca gggggtatcg atgagttcgc caacgggcag
    10501 tgagaaatcg ggaaatccgt cttgttcttc tccgcaagcc gtgtcgttct cttctaaaaa
    10561 ctcctgttgt aaaccaacat cctgttcttc cccttcctct tctccgtcaa gaggagggag
    10621 atcatccagt agatcgtcgt tggattgtgc ggccgcctgc atggttggaa gcataatgca
    10681 gcaatgtcgt aaagggataa gtcaagttgt tttccatgat tcgcgcgatg gcgatggggg
    10741 cgtttcgaag ccgtgtcatg gaaccgtctt gcaaaaacgt cgcggcagtg acagggaacc
    10801 ggtctccgaa caaagtgagc gcgttgagga agcgatgtcc gttcccattc ccacggtcga
    10861 tgacgatgcg ctggtatggt accgaattcg tgttgtcgaa aaagatgtgg cattcgtgcg
    10921 aggcattctg gaagccagcg aaggattggc gtgtatgttc gcggaaaaag gcggcgacct
    10981 gatgcttgtg agtccctcga gtcagcagca acggttggag gaagtgatcg aggacctacg
    11041 tgtggagctt ggtgcgatag tcactgaacg aggaacgaac ggtgggagga agcgcgagtc
    11101 ttcttcgtga gatctcgggg gaggaattga tgcgaagcgg tgacttcggt aggttggaac
    11161 tcatgtcgtc gaccccgtca cgcgaggcga taaatgatgc tgacgtttcg cgcagtcgcg
    11221 tcgagggagc gtggcgtgtg tggttggaag ctttggcgac cgatgccgag gccgcgttgg
    11281 gggcatctct tgcctatgaa gctttggatg aggggggaag aaatgcggtg ctcgatatct
    11341 tggaaaagga tgtggcaacg ctggccgttc caagaattgc cgtgtttgcg cctctgctat
    11401 cggtcgaacg ggacagtcaa cggagagacc ggatacggat agccatcgga tcggacatgg
    11461 atgaacggag gcaaatctcc gccagagcac tcatgggaac cagaagcgac ggggatcaaa
    11521 tcattgtggc agaaacgcat gcgtacttgg atttcgttcg actgacggtg tgtcgactgg
    11581 accctgatca tggtatccgt tgggttcgaa gtgagcctct cgttcatgca cgggatgcta
    11641 ccaaaccagg gacccggatg tacgaagcgg atctcgagcc acttccggtc cgtacggcgg
    11701 tagatatcat cgcgcgcgcc atcgttgctc aacgacgacg ggatggggtt cttccgccga
    11761 gcttgcttcc tttgctcgaa ctattcgatg tcgggacgga ggatccgctg ggggggaagg
    11821 atggctacgt caccgaaggc aaaggttgaa cgctggatgc ggcatcggcc tttccgtggg
    11881 ttgctggcaa ttctctttgc tctttgggga tgcggcgggg cggcaattcc tccggctcac
    11941 gacgaatcgg aagagggttc ccatgcggta cgcttgcttc cttcgggaga cacccctcga
    12001 tttcgatttc gcgccttgga tggacgcgaa gtatcaagtg acgtggctct tggacgaacg
    12061 agtgtgattg cttttatggc aaccttcgat tgggcttcgc aggcgcaagc caggttcatc
    12121 tctggtatcg agcgtcatca tacgccgagg gtgaattgct ttgggattgt gatggagcag
    12181 gcagaaaacg ctcctttggt ggaatcattt gtgacgacac tggggctacg ttttccggtg
    12241 gcccatgtga cttcctcgca ggtaggcaaa gcaacgttgg gagttcgaac cgttccgacg
    12301 gtgctcgtga tggatgcggg aggcaacgtg atttggcgaa gtcgtgggct tgctacggag
    12361 gaccagcttc gggaggtgct tcacgcggtc gaagcgcaaa cgaagaagaa agtgaaatag
    12421 ccctctggac atagaacgaa agccatcctt aaagtgacag ggaagcgttg aaacacgagg
    12481 aggtctccgt gaagcacgcc gtggctcatg atttatcgat tccgctggcg aaagcggccg
    12541 tgaacaaggc attcgaatcg tattgtgcga gattcgccga atacaaacca acccttagat
    12601 gggtgagtga ccgtaaggcg gacgctgggt tttccgtcaa agggttttct ttgaagggcg
    12661 gattcgaggt gacggattcg acgatcgaat tggaactcga tgttcctttc gttcttcgga
    12721 ttttcaaaaa ccaggcgtta tccgtgatcg aaaaagaggt tttgctttgg gtttccaaag
    12781 ccaaggcggg tgaaatcacc gtgtagaaca ccgagcgact ggctggctga tcaggtcatt
    12841 tcttcaaaca tggccatggg cccaacctgg cgtcgctccg aaaacgccac agcaccaaac
    12901 caacagcgtc gcgggagtcg gtgccttgtc aggcgagcac gatccgtatc gcatggcctg
    12961 agccaacagg cgtcataggc ccgctcctgt tcgcacgagg ttaggccgaa gggtgctctc
    13021 tataccaagt gatacttggc gttcgcacgg tctccggttt tcgagatttt tccttcggcg
    13081 acgagcgcct cgagggtgat gcgaagggtg cgagcatctt tgccggtgag ttttcgcaaa
    13141 tcggaaatgg aaagcccttc ggcatcgagt tgatcgagga cgaattgctt gtcttcgtca
    13201 gccgggctat ccgcgacgtc atcttttttg ggtttcgggg cgctgcgttt gattttgtat
    13261 tcgacgcgca tggccgtaag gatggcatcg tcactcagct tggcggaaag gagcactttc
    13321 aattgctgct tggccgcgtc gacctgattt tcgaccatct gcaggcaaga tttcttttcg
    13381 gcgattttgg cttctaagac tgcgatttcc tgctgcaatt tggctcgttc atcgtctaag
    13441 cgtccggtcg aaaaccctgc ttcgtcttcg aattggagcc atagaatctc gaatctttcg
    13501 ccgtcgaccg gaatctgatc tggatccgag cttttggtgg ttcgaagggg aacggttttg
    13561 gtgggcggtt tgttagggcg tggttgggtc atgttgcgac tccgcgaacg tggaaccatc
    13621 aagcattggg gccgaaagat agaatcaaat aacccccaga gcgttcgtct ttttgagttt
    13681 cgagcaggcc acggcgctgg gcgtcttcga tcaactcgtt gaacgtccta taaccgtgaa
    13741 aagattcgga aaaattgggc ttttttcgtt tgagggtctg cttcaccatc gagccccaca
    13801 ggttatcgtc tcgatcacga aacaaagact ccacgacgtc gagcaccatt tcgatcgctt
    13861 cctcccgtcg ttcatcgaca cttttcgtat ccatgtccac cgcgggcgag gaagcattgg
    13921 tggaatccgg ttttaccaac tcaggcttcg gttggccttc cgaccgagtc gctttggcgg
    13981 gcggcttcgc cggtgccttg cccttgttcg cgtcgcgctt cgccgtgggt ttgatgcccg
    14041 tgcgaagaga tcgaatcaga tcgtcgtagt agatgaactc atcgcagttg ccgacgagca
    14101 ggtcgctgct ggagtttttg actcccaaac cgatgacctt cttatcgttt tcccgaagct
    14161 tgctgaccag aggggagaag tcgctatcgc ccgatatgat cacgaaaata tcgatatgcg
    14221 ttttcgtgta acaaagatcg agcgcatcca cgaccagtcg aatgtccgcg gagttctttc
    14281 ctgagtagga aatatgcggg atttccacca gctcaaaagc cgcttcatgc atggaacgct
    14341 tgtgggactt gtatcgatcc cagtccgcgt aagctttttt aacaacgatc tttcctttgt
    14401 ccaaaagacg ttcgagcacc aagctgatgt tgaagggctc gacgttggca tcgcgaacgc
    14461 caagagccac gttctcgaag tcggcaaaga tggcaatatt cggggcagcg tcttcccgtt
    14521 gcgtcatggg ggcgcatcct agcagtgcgg tggaaaaaac gggttccaac taacccaaaa
    14581 agcggttttg gaggacgaga ggaacgggat tccgcttgta tgatagggaa aaccctcaat
    14641 attttttttt tgagaacgta acgccaagct ttccggtatt ctcttgccat ggtacgaaaa
    14701 caaccgaatc gactcacatg ggtagtgggc tcgttcttcg tcttggtcgg cgcttgttcc
    14761 attgtggctg aggtcgaccg ggaaaatatt ccctccaacg agactccgag cacgggcggc
    14821 tcgggaggaa ccggcggcgc tgggggagct ggtggcacgg gaggagtcgg agcaaccgga
    14881 ggatccggag gcgctggtgg ttgcacggtc ccctctgact gtccaggaac ggataccgac
    14941 tgtgcttcgc gcacatgcgt tggcaattcc tgcggggtcg aatacgcggc cgccggaacc
    15001 ctgacctccc agcaagtgga aggagactgt cagtcgctcg tatgcgatgg gtccggaagt
    15061 acgatcaaag tcaatgacga taaggacatc ccagctcagg acaacgagtg cgctgtcggc
    15121 tcgtgtgtgg cgggagcgcc aaaggtcgac tttgcgcccg aggggaccga atgctcttcc
    15181 aatggcggaa atgtatgtga tggcgagggg
//
LOCUS       DAOSOR010000059        15007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4004_length_15007_cov_4.734350, whole genome shotgun sequence.
ACCESSION   DAOSOR010000059 DAOSOR010000000
VERSION     DAOSOR010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 15007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15007
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4004_length_15007_cov_4.734350"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1439
                     /locus_tag="PK710_05000"
     CDS             132..1439
                     /locus_tag="PK710_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09105.1"
                     /translation="MIRRIHVLLLTCIRALVGCCNSDPPAKDPQIEQPVASAPTARPS
                     RCQPIGQDYWVGSRSPSSDDLDKILPFATELGDGVAFDGGFAVGALSQQGQGTAFEII
                     TTNRDGTAWRKISAGASHGDADAPRVFSRGKTLGMAILEPSGSSRTLRLARIDRDQIL
                     WASEILQDQDESLAYDVVIGKEVAVAVWDGVPKGRDVGAVFLATFDPTTFQKPSKPKV
                     MTLPGTDAEMPRLLERSGGFWLFWLARRPGSPSDDSQYRAEDIDYRWIEALPLDNRGA
                     MTGTAVRLGPTDGHVLGFDVVSLQDDSAMVVWRDDDTPSGSPGGQLFMSRVRLGGVEG
                     PERIDEEHTGVGAPNVMPGWFAIADALGSTRLAPLSPDGKLTDKLLRESLLGSGEPIA
                     NHGNDLFVSRPEGTAMRLFVARCLSTAIDGGSQDEEESRDAEP"
     gene            1458..1850
                     /locus_tag="PK710_05005"
     CDS             1458..1850
                     /locus_tag="PK710_05005"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HOT09106.1"
                     /translation="MEQQLDVKVYYEDTDCMGVVYHANYLRFLERARTELLNSLGPSI
                     REWTDRGVIFPIYSVQITFKAPARLGDTLRVYTRAKKTSPFRVTFDQRIERPEDGKLL
                     IEAKVEAVCTDLQGRLREYPDIGLQHGS"
     gene            1840..2670
                     /locus_tag="PK710_05010"
     CDS             1840..2670
                     /locus_tag="PK710_05010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT09107.1"
                     /translation="MAPDSPSEQIQIFSELPADHASFDISDPRVMAALSRAEELHFWH
                     RSRNEFIASRLRMLGIGAGQRFLDLGCGAGCVSAHLSILGLSVVGVDGHRHLLLRAAQ
                     RAPKAKFWLHDLTRGVDELPPDRFDAVGLFDVLEHLDHPSQTLRQAADLVRSNGFVVG
                     TVPAMMSLWSRADEQAGHVRRYETAELHRLLRSNQDCHVVEIVPFHRVLIPLWWMRKL
                     MLARRRDGASVSTTNLRVPPWPINEWMLRLLRVERALSRGPSAWSQHGTSLWFALRKA
                     "
     gene            complement(2685..2758)
                     /locus_tag="PK710_05015"
     tRNA            complement(2685..2758)
                     /locus_tag="PK710_05015"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2722..2724),aa:Met,seq:cat)
     gene            3011..5401
                     /locus_tag="PK710_05020"
     CDS             3011..5401
                     /locus_tag="PK710_05020"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOT09108.1"
                     /translation="MKKIQGEDDRGAPDFRGQRKLSIWPFLGVLTVTLAAAIAPGCAV
                     GAPEEEEPIASVEAYLTLAERMARYEKIRDAAAARGITSTAYLLAGIAHAETGLAHCW
                     SEATWACQGPYSPDCNGPVIAGAGDGPCSLQQGGLGMFQFDAGTFTDTLNKYGNDVLT
                     IAGNTSHAINYVINMVKVSAYTTNAETDAKALQWINNFDFGNGTLRDQWIKTVTRYYN
                     GCQPGWFCWSDRYNHYNNSLQTVVNETGLDFWIANTCKPEPEVCNGKDDDCDNEVDED
                     DVCEKEALLLGQSWVAPARTTDIDGDGLMDVCGRGMAGVWCHLSQATSWGENGPVLEL
                     SDKSGWDDPTNWGTMRWGDIDGDGRADLCARANANVDCWKSDGTKLATHVVGPGWSDD
                     SGWNDFKYHSTIRLLDIDGDGKDDLCARAAKGVVCYRSTGNGFDTAVDGPAWSNEAGY
                     GEPKFYGTLRTGDVNGDGREDLCIRSKDGMECWLSNGSGFPTRVQGPNWSDEWGWSAY
                     RYWSTIRLVDVNGDRKADLCARAAAGLRCHLSQGDSFGEAIEVASLSDDSGWNDPSNF
                     LTLRTGDIDGNGTQDLCVRANAKIHCYLWNGNGFDAIEGPDWSDADGWNEKKYYHSIQ
                     LADMNGDGRADLCGRHVTGWRCHPSTGNGFGDAIELDEFTDAGGWGVSKYFATVLIGG
                     TGCVPSDEVCNGKDDDCDGLVDEDGVCDETGGTGGATGSTGGAGGSQNLGAGGTMAGG
                     SAGKGNDVDTVSHGDWYSSNSDEGCTCRLAPKTQGNHAMVGWFLLALTLWYRSRRR"
     gene            5909..8245
                     /locus_tag="PK710_05025"
     CDS             5909..8245
                     /locus_tag="PK710_05025"
                     /inference="COORDINATES: protein motif:HMM:NF023885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR-associated protein Cas10/Cmr2"
                     /protein_id="HOT09109.1"
                     /translation="MSIDWNQKLAWALRDLPDPNLDILQLSAHRATLLGSEDLSADVG
                     NRADWLLCSADRVPFPSGSSAARVQVDWAALPEVTHPLAKERFVAQPFDLTAAQNVVA
                     ELLAECASRFDRDARRMLLWIWRYLPERLAVAEKGPGPIASQFPADPRLPNLSLWQRF
                     SFDTALATASPSPCFLVLNISSLEAACAESATPEDLWTVGSMAFELIWHALLPIIETY
                     GPTAILSPCLRGNPKLDAWLAEQGISAAQDVSAPALTPSTMIGLPNVVVALVEADQAQ
                     SLGEACKQRFDDHWASVVSAIRDKLTKAKWAKQSNESWKGSWSRQTPRAWELHWTAVR
                     WADDSTGAGNLLSKQEVSALEKWARVQESAAGLEDPWPGIYYGLWYSAALAASGARQR
                     LGSISVLSEPSPTCSSCGHREALHDGSGDVQAFWAPLVGPKGKTSGAVHGDERLCAVC
                     TMRRLAGQLGSTTVPEGLRALANEPSYAVMVLSLDQPFALLRGGKELKQMASLADCVH
                     SELDKGFTKRARAHVKEVLDTPALLGPARQLAVQEALDESMAALAEIIHQAGAFALQI
                     SDREIILLAPTHRVYDLARATHALLQTSFAQVPTHGGTKLALRVGSVASHSAVITIVP
                     GAEAPGPVLKDCLEKLQDLARAQLGGDALVITRRSVHEEERLFAAHWNDLAASMDILM
                     ASMPSVSSLQRLAQSLSHVAPALSNADLDKSSVMARASLVVTTLEELSLEENLDHEAI
                     ARAVCTLVDHCVRCPGGMEENHALDALHIAASLRGAGQ"
     gene            8242..9213
                     /locus_tag="PK710_05030"
     CDS             8242..9213
                     /locus_tag="PK710_05030"
                     /inference="COORDINATES: protein motif:HMM:NF021236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated Cmr3 family
                     protein"
                     /protein_id="HOT09110.1"
                     /translation="MKTDVLIVPNDTLVLALGHGPRGRYMRSFDGRLPPDLFLQMLHA
                     AYASCGLQTPRTMRITAQLVAMGVGGRLEPLYPVPDDLVVEPGAKDAVWAQPAYFEMV
                     SEDVVQHDMGNLHLLVALSRNPDTHPFDAFLTRKGVRVWAERKPQRLTEGEHYLPLSR
                     LFEIELRGSGEQTAAHLFVKPGVGFLVSFETPDDSPTLPLPMTVRASLENRSVQIEEA
                     QYPLPTIETKSRKRWRLCLLSHLHVENGGWPPWVDGATLTTRAPLPEGGKVVAVARRR
                     GENVVGAPFNTTPGPAQTIIPIGTTLFIEFEEPVQVEQSAEILAGGS"
     gene            9213..10055
                     /gene="cmr4"
                     /locus_tag="PK710_05035"
     CDS             9213..10055
                     /gene="cmr4"
                     /locus_tag="PK710_05035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr4"
                     /protein_id="HOT09111.1"
                     /translation="MMSTDSVVLSLYFETPLHAPSGSVVGTIEQPVQRERTTQWPVIY
                     ASTLESSLRTSFMQTAGEQAAKALFGDDNGGGLSISDARLLLFPVRSSAAPFLWLTCP
                     AVVSRLRRDLGRTVGIQIPPAPSVKTDEIVTSQAWSHGDSPLAADDIVLKPRAGYDAS
                     KLMALLPSDSSAYDVFAKEVEASFGLVSDEVFGFLVRTGTEVRVIDGVLGDEGNPCYD
                     EAIPADSLFYVPIIGIGTKEAKATALTRFAEGCGTHVRVGNGEPIGRGWARTRLLKAE
                     GSAQ"
     gene            10052..10417
                     /gene="cmr5"
                     /locus_tag="PK710_05040"
     CDS             10052..10417
                     /gene="cmr5"
                     /locus_tag="PK710_05040"
                     /inference="COORDINATES: protein motif:HMM:NF021237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated protein
                     Cmr5"
                     /protein_id="HOT09112.1"
                     /translation="MKTREQLRAERASSMVAALKADAIRDYSIEVRAFPSLVHQAGLA
                     PALAILKAKGGQAKSFLYDHVSAWVSETTFADPKGDALSLIIKNPAIHLLRAQEEALA
                     FCSWLRRFAEAREPKARKA"
     gene            10414..11553
                     /locus_tag="PK710_05045"
     CDS             10414..11553
                     /locus_tag="PK710_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09113.1"
                     /translation="MTRALPVDLGNLPDSEGAAAANPSLQLVRWHQWGNNWSLDDDQE
                     QSRDLDAMVVFDTDSSVRNVLQKGLEQLRARAAGRARALKARGLQVLKLRLPCSGRVL
                     RMTGDAPPGEIGFTLHRSLGVPYMTAPMVRFIMRVATSQSAELVWPEHLREESEQQQI
                     LQQLHPSSLSDPPCPSAIIVFDAFPVKLPVVERRKSDLRWTIANGLTPEEPLMTASEL
                     RRRQALCVDMDTELEFWFAAPDAQLLELAQRHLELGLVLLGLQLPPVVEEPEPEPETQ
                     ESEANDDEEEISPENMPPTEISPDVATITVAVPTVQYLPNNGRVVVMFLPPGGRDHMK
                     AEEHINGIVMPDDLRKRLKKKKVITQVRVEVEAVGNSWKIRGLQQ"
     gene            complement(11562..12317)
                     /locus_tag="PK710_05050"
     CDS             complement(11562..12317)
                     /locus_tag="PK710_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT09114.1"
                     /translation="MMYNNAFITGASSGLGLALSKRLSRQGTRVILAARRESELRSAA
                     ESIRADGGQAQVCVLDVSQPEAVFESIRWWDQQTGGFDLVIANAGVGRTKAAYRLDWK
                     DVSAVLQVNVMGAFATLFAGMECMLKRKRGTLVGISSLAGMRGLPKTGAYSASKAALG
                     TFLETLRADLASQGIRVVDVRPGFIDTAMTKGNRFKMPFLLDVERAAEMTLQGIHRGM
                     PVVSYPWPVASTMYLARSIPDLAWGVIARRMRY"
     gene            12475..13533
                     /locus_tag="PK710_05055"
     CDS             12475..13533
                     /locus_tag="PK710_05055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT09115.1"
                     /translation="MNHARTAVRIAIVATSYPSHPLDPTGHFVRAESLALVEQGHDVH
                     IIAPAGPEGLLSNDEGLTSWPITHAGAFGWPGAMSRLREKPWRLFGAAAFAALASDRL
                     RRLRPTRIIAHWMIPCAIPIASFSRITERVDVVSHGADVRFLLSLPAPVRERIVGNVL
                     SLASSIRFAAHATYAALAHSLPAELQTRLHAKAVVALPTLNVPDVSLEAKRLRQQLGS
                     SPLAVTVSRLVASKRVDLAIAAARHVRPPFRLGVLGDGPEREKLSRLPGAEHAIFQGF
                     LPRNQALAWIAAADVLIHASTTEAAPTAIREARQCRIPVVACDAGDVALWAQRDHGIV
                     VVPPSPSAIASAVNQLIA"
     gene            13544..14293
                     /locus_tag="PK710_05060"
     CDS             13544..14293
                     /locus_tag="PK710_05060"
                     /inference="COORDINATES: protein motif:HMM:NF017551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HOT09116.1"
                     /translation="MCTLLIAQQFVDAIPVVVLANRDEFLDRPSLPPALRDRVPPVFC
                     GLDAREGGTWFGINAKGLVVALTNLSVRPPDRSLRSRGLLCMDLLALEDAMSAERALR
                     HLEANSYNPFNIVAIDTTTAVRAVYDSRPDVQRMPPGIYAITNWPLDSPHDSKREGLE
                     TRVRSRLSPQATAAEVIDLLQNEARMHLEDADPRTSVCCHSPLYGTRASSIVLLSDTH
                     VRMQHADGPPCSTPYENLSASLARMMSFKIF"
     gene            14348..>15007
                     /locus_tag="PK710_05065"
     CDS             14348..>15007
                     /locus_tag="PK710_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09117.1"
                     /translation="MAAIDCPKRSLILLLTALACSDATSDDASKPSSPPEADAAGVSL
                     PCTDTADPLDLDGLFALSARLNFSFASQPGGAVTVCPTDQTSDGHFLGFVRVQHVAGQ
                     TEVPSVSAVICSIDLPVISAIVGECVPQASNVVYAGLEFPKPLIESLPVASSALTTAQ
                     LSSPNPGATLSAGSMNFSIGTNERIASAPGWLMTHSGCGMNDVAPGRSMECEQTCVTD
                     CS"
BASE COUNT         3072 a         4622 c         4124 g         3189 t
ORIGIN      
        1 gaatcatcga aggatgggtt agtccttcgc ggggccggag gcaaaggttt tggcaggggg
       61 aatgccgccg gtcaacgctt ggtttgcaga acctttgcct ccggccccgc gaaggactaa
      121 cccatccttc gatgattcgc cgcatccacg tgttgttgct cacatgcatc cgggcactcg
      181 tcggatgctg taacagcgat ccgcccgcca aagaccctca aatcgagcag ccggttgcgt
      241 cagctcccac ggctcgtccc tctcgctgcc aacccatcgg ccaagactac tgggttggtt
      301 cacgctctcc aagctcagac gatctcgaca aaatcctgcc tttcgccacg gaactcggag
      361 atggagtcgc cttcgatggc ggatttgcag tcggagccct cagccaacaa gggcagggaa
      421 cggcattcga aatcatcacc acaaaccgcg atggcacggc gtggagaaaa atatctgccg
      481 gcgcctctca cggcgatgcg gatgcgccac gagtcttttc ccgcggaaaa accctgggta
      541 tggcgatttt agaaccctcc ggatcttccc gaaccttgcg gctcgcacgc atcgaccgtg
      601 accagatcct gtgggcaagc gagatcctgc aagatcagga tgagtccctc gcttatgacg
      661 tcgtcatcgg aaaagaggtg gccgtggccg tttgggatgg cgttccgaaa gggcgtgacg
      721 tgggagccgt ttttcttgcc accttcgacc ccactacgtt ccaaaaaccc tcgaaaccca
      781 aagtgatgac actgcccgga accgatgcgg aaatgccacg gctgctcgaa cgctccggtg
      841 gattctggct tttttggctc gctagacgtc ccggttcccc ttccgatgac tcccaatacc
      901 gcgcggaaga tatcgattac cgatggatag aagccctacc cctggacaac cgcggcgcaa
      961 tgaccggaac ggcggttcgc ctcggtccca cagacggtca tgtgctcggc ttcgacgttg
     1021 tatcgttgca agacgattcc gccatggtcg tctggcgcga tgacgatacc ccgagcggct
     1081 caccaggtgg tcagctattc atgagccgag tccggttggg aggcgtggaa ggcccggaac
     1141 gaatcgatga agagcatacg ggtgtcggcg ctcctaacgt catgcccgga tggtttgcca
     1201 ttgccgatgc ccttggatcc acgcgcctgg ctcccctctc cccagatggg aaactcaccg
     1261 acaaactcct gcgcgaatcg ctcctcggat ccggtgaacc catcgcaaac cacggcaacg
     1321 accttttcgt ctctcgaccg gagggaaccg ccatgcgcct gttcgtcgcc cgatgcctta
     1381 gcaccgcgat cgatggtgga tctcaagacg aggaagagtc ccgcgacgct gagccgtgaa
     1441 aggcgtatgc tcccgccatg gagcagcagt tggacgtcaa agtatactac gaagacacgg
     1501 attgcatggg cgtggtgtat cacgccaatt atttgagatt cctcgaacga gcacgtacgg
     1561 aactgctcaa cagcctggga ccatcgattc gcgaatggac cgaccgtggg gtgattttcc
     1621 caatctatag cgtccagatc acgttcaagg ctcccgcacg gctcggcgat acgttgcgag
     1681 tatatacccg tgcaaaaaaa acatccccct ttcgcgtgac cttcgatcaa cgcatcgaac
     1741 ggccagaaga cggaaaactt ctcatcgaag cgaaagtcga agcggtatgt acggacttac
     1801 aggggcgcct tcgagaatac ccggatattg gtttgcagca tggctcctga tagcccgtct
     1861 gaacaaatcc aaatcttcag cgaattgcca gcggatcatg ccagcttcga tatctccgat
     1921 cctcgcgtca tggcagcgtt gagccgtgcg gaagaactgc atttttggca tcgatcacgc
     1981 aacgagttca tcgcttcgcg gttgcgaatg ctcggaattg gcgcaggaca aaggtttctc
     2041 gatcttgggt gcggagcagg ttgtgtgagc gcgcatcttt cgatacttgg cctttcggtg
     2101 gtgggtgtcg acggtcatcg gcacttgctg ttgcgcgctg cgcaacgagc gcccaaagcc
     2161 aagttttggc ttcacgatct gacgcgaggc gtggatgagt tgccacccga tcgtttcgat
     2221 gccgtggggt tgttcgacgt gctcgaacat ctcgatcatc cctcgcaaac cctacgacag
     2281 gctgccgatc tggtgcgatc gaatggattc gtcgtgggga cggtgcccgc catgatgtct
     2341 ctttggtcaa gggcagacga gcaagcgggt catgtcagac gctatgaaac ggctgaatta
     2401 catcggttgt tgcgctcgaa tcaggactgc cacgtggtgg aaatcgtgcc ttttcaccgt
     2461 gtgctgatac cgctgtggtg gatgcgaaag ctcatgttgg cacggcgtcg tgatggggct
     2521 tcggtgtcca ccacgaactt gcgtgtgcct ccgtggccca tcaacgaatg gatgcttcgc
     2581 ttgctccgtg tggaacgggc tttgagtcga ggcccctccg cttggagcca gcacggcact
     2641 tccctctggt tcgcattacg aaaagcgtaa gccgatttcc cgggtgccaa ggggcggagt
     2701 cgaaccgccg acctagcgcg tatgaaacgc ccgctctgac caactgagct accttggcga
     2761 aaggcgacgc attgtacggt ggaactaggc cgtgtcaagg gaacttgccg acggaatgat
     2821 cgattccact cccgtggggc cgtctttcag gagatccgag atccgaaccg agatcccgga
     2881 gatcgcactc gcttgcggtg ggggtggtgg cttgcgcgca ttttccactc gaatgacgaa
     2941 aagcttggtt ggtccgattt gtgcttacag tgtccaatgt cgtgaattca gcccgttttt
     3001 ggagcagcga atgaaaaaga tccaagggga agacgaccgt ggagcaccgg actttcgagg
     3061 ccaacgaaaa ctgtcaatat ggccttttct gggcgtgctt accgtcacct tggcggcggc
     3121 gattgcgccc ggatgcgccg tgggtgctcc ggaggaagag gagccaatcg catcggtgga
     3181 ggcgtacctg acactcgccg aacgcatggc gagatacgaa aaaatccgcg atgccgccgc
     3241 tgcgcgaggt atcacctcta cagcgtactt gcttgcagga atcgctcatg cggagaccgg
     3301 actcgcgcat tgctggtcgg aagccacatg ggcttgtcag ggaccctact cgccagattg
     3361 caacggaccg gtgatcgctg gtgcagggga cggaccttgc tccttgcagc aagggggcct
     3421 cggcatgttt caattcgatg ccggaacctt caccgatacc ctcaacaaat atggaaacga
     3481 tgttctgacc atcgctggaa acaccagcca cgcgatcaat tatgtcatca atatggtgaa
     3541 agtctctgcc tacaccacca acgcggagac cgatgccaaa gcattgcagt ggatcaacaa
     3601 cttcgacttc ggaaacggaa ctctgcgcga tcaatggatc aaaacggtca cccgctacta
     3661 caacggatgc caaccaggat ggttctgttg gtccgaccga tacaaccact acaacaacag
     3721 ccttcaaacc gtcgtcaacg aaaccggtct ggacttctgg atcgccaaca cgtgcaaacc
     3781 cgaaccggaa gtctgcaacg gaaaagacga tgactgtgac aacgaggtgg acgaagacga
     3841 tgtgtgtgag aaagaagcgt tgctgcttgg ccagtcttgg gtagcaccgg cgcgcacgac
     3901 cgatatcgat ggcgatggtc tcatggacgt ctgcggtcgc ggtatggcgg gcgtatggtg
     3961 tcacctgtcg caggcaacat cctggggcga aaatggacct gtcctcgaac tcagcgacaa
     4021 aagcggatgg gatgacccca ccaattgggg aaccatgcgc tggggagata tcgatgggga
     4081 tgggcgcgcc gacttatgcg caagagccaa tgccaacgtg gattgttgga aatccgacgg
     4141 aacgaagctg gctacacatg tcgtcggccc cggctggtcc gacgatagcg gatggaatga
     4201 cttcaaatat cactctacca ttcgtcttct cgacatcgat ggtgacggaa aagacgatct
     4261 atgcgccaga gctgcaaaag gtgtcgtctg ctaccgatcg accggaaatg ggttcgatac
     4321 ggcggtggat ggacccgcgt ggtcgaacga ggcgggctac ggtgagccga agttttacgg
     4381 tacgttgcgg acgggagatg tcaacggcga cggtcgggag gatctatgta ttcgttcgaa
     4441 agacggcatg gagtgttggc tttcgaatgg atcgggattc cctacgcgtg tgcaagggcc
     4501 gaactggagc gacgaatggg gatggtcagc gtatcgatat tggagcacca tccgattggt
     4561 tgatgtcaac ggcgaccgga aagcggattt atgcgctcgg gctgccgctg ggctccgttg
     4621 tcacctatcc caaggggatt ccttcggtga agccatcgag gtcgcatcgt tgtccgacga
     4681 cagcggttgg aatgacccat cgaacttcct gacgttgcga acaggagata tcgacggcaa
     4741 cgggacacaa gacctatgcg tgcgagccaa tgccaaaatt cactgctacc tgtggaatgg
     4801 caacggtttc gacgccatcg aggggcccga ttggtccgat gcggacgggt ggaacgaaaa
     4861 aaaatactat cactcgattc aactcgcgga catgaatggt gacggacgag cggacctttg
     4921 cggtcgtcat gtcaccggct ggcgctgcca cccatcgact ggcaacggat ttggcgatgc
     4981 catcgaactc gatgagttca cggacgcggg gggttggggt gtgagcaagt acttcgccac
     5041 cgtattgatt ggaggaaccg gatgcgttcc atccgacgaa gtgtgcaatg ggaaagatga
     5101 cgattgtgac gggctggtgg atgaagatgg cgtatgtgac gagacggggg gaacgggcgg
     5161 agcgactgga agtacgggag gggctggtgg gagtcaaaac ttgggggcag gtgggacgat
     5221 ggcgggaggt tccgcgggaa aaggcaatga cgtggacacg gtatcccacg gggattggta
     5281 ttcctccaat tctgacgaag gctgtacatg ccgattggca ccgaagacac aagggaacca
     5341 tgccatggta ggatggttct tacttgctct taccctgtgg tatcgctcac gccgccgcta
     5401 agaccagttc cccgcatctg caacccttct ttcacgacaa atttgagaaa tattcgtagc
     5461 tatcgacaac gaatcggtct atctaacacg ccccgccacg gacccattgt tctcaaggta
     5521 tggtaagtga ggaatttcgg gatattccta tcagcaagct tgtgcccttc aggctcgtcg
     5581 ttctgggacc gtggcgacaa gggccgggac tcccatgccg ctttcctacc atccacatgg
     5641 attcttccat ggacctgacg tcgcgattcg tatatgggcg cgaagtgcgt gaacccagcc
     5701 catcgtttcc gagccttgat ccttgctgtc gacgaaacag ggataagctc ggctcagcca
     5761 ggcccacgca agccagtccg aggaattctt tctgttttgc ttcaatcatg actcgctctg
     5821 gcaccttgta ccccttgacc atgaccgcct ttgagcgcgc gtttttcgcg cttgcgggct
     5881 catcgacatt ccgttgacgg aggcgcactt gtcgatcgac tggaaccaga agctcgcctg
     5941 ggcattgcgc gaccttcctg acccgaacct cgatatcctt caactctcgg cccaccgggc
     6001 gacgctgctc ggttccgaag acctttctgc agatgtcggc aatcgagccg attggctctt
     6061 gtgttctgcg gaccgtgtcc catttccttc cggctcttct gccgcgcgtg ttcaggtgga
     6121 ttgggctgca ttgcctgagg ttacccaccc gctggccaag gagcgtttcg tcgcacagcc
     6181 gttcgatctc acggccgctc aaaacgtcgt cgcggaattg ctagctgaat gtgcctctcg
     6241 cttcgatcga gatgcgcgac gaatgctcct gtggatttgg cgatacttgc ccgaaagact
     6301 cgccgtcgcc gaaaagggtc ctggccccat cgcttcccaa ttccccgcgg accctcgtct
     6361 gcccaaccta tcgttgtggc aacgattctc tttcgatacg gcgttggcga cggcttctcc
     6421 ttcgccgtgt ttccttgtgc tcaacatctc ttccctcgag gccgcttgcg ccgaatcggc
     6481 tacgccagaa gacctgtgga cggtgggctc gatggctttc gaactcatct ggcacgctct
     6541 attgcccatt atcgaaacct atggtccaac ggccatcctc tctccttgtc tccgtggcaa
     6601 cccgaaactg gatgcttggc ttgcggaaca agggatttcg gcagctcaag atgtgtcggc
     6661 accagcgctg accccctcga cgatgatcgg tttgcccaac gtcgtcgtgg cgctcgtcga
     6721 ggcggatcag gcccaaagtc tcggcgaagc ctgtaaacaa cgcttcgacg accattgggc
     6781 gagtgtggtg tctgctatcc gcgacaagtt gacgaaagcc aagtgggcaa aacaaagcaa
     6841 cgaatcgtgg aagggctcct ggtctcgaca gacgcctcgt gcgtgggaat tgcactggac
     6901 ggcggtgcgc tgggccgatg attcgacagg agccggcaat ctgctgtcca agcaagaagt
     6961 atctgctttg gagaaatggg ctcgtgtgca agaaagcgcg gcgggactcg aagacccgtg
     7021 gcctggtatt tactacgggc tttggtactc tgccgcgctc gccgcctctg gggctcggca
     7081 gcgtctcggc tcgatttcgg tgctttccga accctccccg acttgttcct cttgtgggca
     7141 tcgcgaggcg ttgcacgacg gttccggtga tgtgcaagct ttttgggctc cgctggtcgg
     7201 tcccaaaggc aagacctccg gcgccgtgca tggggacgag cgcttgtgcg ccgtatgcac
     7261 catgcggcgt ctggctgggc agcttggctc aacgaccgtc cccgaaggat tgcgggccct
     7321 ggcgaatgag ccatcgtacg ccgtgatggt gctgtcgctc gaccagcctt tcgcgctgct
     7381 tcggggaggc aaagaactca aacaaatggc ttcgttggcc gattgcgtgc atagcgagtt
     7441 ggacaaagga ttcaccaaac gcgctcgcgc tcatgtcaaa gaagttctcg atacaccggc
     7501 tttgctaggt ccggctcggc agctcgcggt gcaggaggct ctcgatgagt cgatggccgc
     7561 tctggccgaa atcatccacc aagccggcgc tttcgctctc caaatctccg accgagaaat
     7621 cattctgttg gctccgaccc atcgcgtcta tgacttggct cgggccacgc acgcgttgct
     7681 acagacgagc tttgcgcagg tcccgacgca tggcggaacg aaactcgccc tgcgcgtggg
     7741 ttccgtcgct tcgcacagtg ccgtcatcac catcgtccca ggggctgagg ctcccggtcc
     7801 ggtactcaaa gactgtttgg aaaagctgca agacctggct cgcgcacaac ttggtgggga
     7861 cgcactcgtc atcacacgac gatccgttca cgaggaagaa cgcctattcg ccgcccattg
     7921 gaacgatctg gccgcttcca tggatatctt gatggcctcg atgccatctg tttccagctt
     7981 gcagcgtttg gcccaatccc tttcgcacgt agccccggct ctctcgaatg ccgacttgga
     8041 taaatcctcg gtcatggcgc gtgcttcgct cgtcgtcacc acccttgaag agctgagtct
     8101 tgaagaaaat ttggaccacg aggcgatcgc acgcgccgtg tgtaccctcg tagaccattg
     8161 tgttcgttgt cctggaggca tggaggagaa tcacgcgctc gatgcccttc acattgccgc
     8221 gagcttgcgt ggagctggtc aatgaagacg gatgtcctga tcgttcccaa cgatactttg
     8281 gtgcttgcac tggggcacgg cccccgcggc cgatatatgc ggtccttcga cggtcgtttg
     8341 ccgcctgatc tgtttctgca gatgctgcat gcggcctatg cctcatgcgg gctgcaaacc
     8401 cctcgaacca tgcgtattac agcacagctc gtggccatgg gagtgggagg acgattggaa
     8461 ccgctctatc cggttccgga tgatctcgtg gttgaacccg gtgcgaaaga cgctgtctgg
     8521 gcacaacccg cctacttcga aatggtcagt gaagacgtgg tccagcacga tatgggcaac
     8581 ttgcacctac tcgtagcatt gagccgtaat ccggatactc atcctttcga tgctttcctg
     8641 actcgaaaag gagtgcgggt ctgggccgaa cgaaaacctc aacggctgac cgagggggaa
     8701 cactatctgc ccttgtctcg gctattcgaa atcgaattgc gaggtagcgg agagcaaacc
     8761 gctgcccact tgttcgtcaa accgggagtc ggttttcttg tctccttcga aacccccgac
     8821 gattccccaa cgcttccgct tcccatgacg gtgcgcgctt ccctcgaaaa tcgatcggta
     8881 caaatcgaag aagcacaata ccccttgccc acaatcgaaa ccaaatcgcg taaacgctgg
     8941 cgcttgtgct tgctatctca tctccatgtc gaaaatggtg ggtggccccc gtgggtcgat
     9001 ggtgcgacgc tcaccacaag ggctcctctg ccagagggcg gtaaggtggt cgccgtggct
     9061 cgacgacgtg gagaaaatgt tgttggggcg ccattcaata cgaccccggg cccagcccaa
     9121 accatcattc ccatcgggac caccttgttc attgaattcg aagagccggt gcaagttgag
     9181 cagtcggccg agatccttgc gggaggaagc taatgatgtc cacggattcc gtcgttttga
     9241 gcctctactt cgagacacct ctccacgcgc cttccggcag cgtggtcggt accatcgagc
     9301 aaccggtgca acgtgagcgt accacccagt ggcccgtcat ctacgcttcc accctcgaaa
     9361 gctcactacg aacctcgttc atgcaaaccg ccggagaaca agcagcgaaa gccctgttcg
     9421 gcgatgacaa tggcggcggt ttgagcatca gcgatgcacg attgctcctg tttccagttc
     9481 gctcttccgc cgctcctttc ctctggctca catgccccgc tgtcgtctcc agactacgaa
     9541 gagacctcgg aagaaccgtc ggtatccaaa tccctcccgc cccttccgtc aaaaccgacg
     9601 agatcgtgac gagccaggct tggtcccatg gtgattctcc tctcgccgcc gacgacatcg
     9661 ttctcaaacc tcgcgcgggc tatgatgcct ccaaactcat ggcgctgctg ccttccgata
     9721 gctccgctta tgatgtgttc gccaaagaag tcgaagcgtc tttcggcctg gtttccgatg
     9781 aagtcttcgg cttcctcgtg cgaacgggaa ccgaagtgcg tgtgattgac ggtgtgctcg
     9841 gagatgaagg caacccttgc tacgacgaag ccattcccgc agactcactg ttttacgtac
     9901 ccattatcgg catcggcacc aaagaggcga aagccaccgc gttgacgcgc ttcgcggaag
     9961 gctgcggtac ccatgtccgc gtcggaaacg gcgagcccat cggacgaggt tgggcgcgaa
    10021 cacgattgct caaagcggag ggaagcgcgc aatgaaaacg agagaacaac tccgagccga
    10081 acgcgcgagt tccatggttg cggcactcaa agccgatgcc atccgagact attccatcga
    10141 ggttcgtgcc ttcccgtcct tggttcacca agccggactc gcgcccgctc tcgccatcct
    10201 caaagcgaaa ggcggccagg caaaaagctt cctgtacgac cacgtctccg cttgggtgag
    10261 cgaaaccacc ttcgccgacc ccaaaggcga cgccctgtcg ctgatcatca aaaatcccgc
    10321 catccatctg ctgcgcgcac aagaagaagc tctcgcgttc tgctcttggt tgcgacgctt
    10381 cgcggaggcc agggagccga aagcgaggaa agcatgaccc gcgccctccc cgtcgacctc
    10441 ggcaaccttc ccgactccga gggcgccgcg gcggcaaacc caagcctgca gctcgtccga
    10501 tggcatcaat ggggcaacaa ttggtcgttg gacgacgacc aagaacaaag tcgtgacctc
    10561 gatgccatgg tcgtcttcga caccgatagc tcggttcgaa acgtgctcca aaaaggtctc
    10621 gaacaactcc gcgctcgcgc cgcaggacgt gcccgcgccc tcaaagcgcg tggcctgcaa
    10681 gtcctcaagc ttcgcctacc atgctcgggc cgtgtgttgc gaatgaccgg cgacgctccc
    10741 ccaggggaaa tcggtttcac tctgcatcga tccctcggtg tcccttatat gaccgctccc
    10801 atggtgcgct tcatcatgcg ggtcgcgact tcccaatccg cggaactcgt ctggcccgaa
    10861 cacctgaggg aagagtcgga acaacaacaa atcttacaac aactccatcc ctcctccctc
    10921 tctgaccccc cttgcccttc cgccatcatc gtgttcgatg cgtttcccgt caaactgcct
    10981 gtcgtcgaac gaagaaaatc agatctgcga tggaccatcg ccaacggcct caccccggaa
    11041 gagcccctta tgaccgcctc cgagttgcgg cgacgacaag ccctttgcgt cgacatggat
    11101 accgaactcg aattctggtt cgcggccccc gatgcacaac tattggaact cgcccaacga
    11161 cacctcgaac tcggactcgt tctgctcgga ctacaactgc cgcccgtggt cgaagagccc
    11221 gaacctgaac ccgaaaccca agaaagtgaa gctaacgacg acgaggaaga gatttccccc
    11281 gaaaatatgc ctccgaccga aatctccccc gatgtcgcta ccatcaccgt cgccgtcccc
    11341 accgtccagt acttgcccaa caacgggcgc gtggtcgtca tgttcctgcc ccccggtgga
    11401 agagaccaca tgaaagccga ggaacatatc aacggtatcg tgatgcccga cgatcttcga
    11461 aaacgcctca agaaaaagaa ggtgatcacc caggtgcgcg tcgaagtcga agctgtcgga
    11521 aactcatgga aaatccgtgg gttacagcag tagcgccccg ctcaataccg catccttctc
    11581 gcgatgaccc cccacgccaa gtctggtatc gaacgcgcca aatacatcgt gctcgccacc
    11641 ggccacggat acgacaccac cggcatgcca cgatgaatcc cctgtaaggt catctccgct
    11701 gcccgctcta catcgagcaa aaacggcatc ttgaatcgat tgcccttcgt catcgccgtg
    11761 tcgatgaacc caggacgaac atccaccacg cgaatgccct ggctcgcgag atccgctcga
    11821 agagtctcca aaaatgtacc caacgcagcc ttgctcgcac tatacgcccc cgtcttcggc
    11881 aaaccccgca tccccgccaa gctcgagatc cccaccaacg tccccctctt tctcttcaac
    11941 atgcactcca tacccgcaaa caacgtcgca aacgcaccca tcacgttgac ctgcaacact
    12001 gcggagacat ccttccaatc gagcctatac gctgccttcg tccgaccaac tcccgcgttg
    12061 gcgatcacca aatcaaaccc gccagtctgc tgatcccacc aacgaatcga ttcaaatacc
    12121 gcttccggct ggctcacgtc cagaacgcat acttgcgctt gcccaccgtc ggcgcgaatc
    12181 gattccgccg cgctccgaag ctccgattcg cgacgtgccg ccaggatcac tcgcgtgcct
    12241 tgacgcgata accgcttgga aagcgccagc ccaagaccgc tggaagcccc agtgataaac
    12301 gcattattgt acatcattct tactcatccg gcgtcggcaa ttcccatccg cgatggccgg
    12361 tcatacctca ccgcccatgg gacgcaaatc gccatcatca gccgtgactc ggctgctggt
    12421 catctaactc gactttctcc ccgtgacgct tctgctcttt gctttccctc ttcgatgaac
    12481 catgctcgca ccgccgtgcg catcgccatc gtcgccactt cctatccatc tcatcctctc
    12541 gaccccacgg gacatttcgt tcgcgccgaa tccctcgcct tggtcgaaca aggccatgac
    12601 gttcacatca tcgccccagc cggtccagag ggtttgttat cgaatgacga aggcttgact
    12661 tcttggccca tcacgcatgc gggggcgttt ggatggcctg gagccatgtc tcgattgcgc
    12721 gagaaacctt ggcgactttt cggcgccgct gccttcgctg ccttggcttc cgaccgactt
    12781 cgacgcctgc gtcccacgcg catcatcgcc cattggatga ttccctgcgc cattcccatc
    12841 gcttctttca gccgaatcac cgaacgagtc gatgtcgtct cccacggtgc cgacgttcgg
    12901 tttctgcttt cgctcccggc tcccgtgcgg gaacgcatcg tgggaaacgt gctgtcgctc
    12961 gcctcgtcca ttcgcttcgc cgcccacgcg acatacgccg cgctcgccca ctcgctgccc
    13021 gctgaactgc aaacacgcct gcacgccaaa gccgtcgttg cgttgcccac cctgaacgtc
    13081 cctgatgttt cccttgaagc aaaacgactt cgtcaacaac tcggttcctc tcctttggcc
    13141 gttaccgtct ctcgactcgt agcttccaaa cgtgtcgact tggccatcgc cgccgctcgt
    13201 cacgttcgcc ccccctttcg tctcggagtg cttggtgatg gccccgaacg cgaaaaactc
    13261 tccaggctgc caggggccga acacgcgatc ttccaaggct ttctgccaag aaatcaagcc
    13321 ctcgcttgga ttgcggccgc ggacgtgctg atacatgctt ccactaccga agccgctccc
    13381 acggccatcc gagaagcgcg tcaatgccga atcccggtcg tcgcttgcga cgcgggagac
    13441 gtggcgctgt gggcacaacg cgaccatggt atcgtcgtcg ttcccccttc ccccagcgcc
    13501 atcgcatctg ccgtaaacca attgatcgcc taagctcttg cccatgtgta cgttgctcat
    13561 cgctcagcaa ttcgtggacg ccatccctgt cgtcgtcctc gccaatcgcg acgagttcct
    13621 cgaccgacct tcgctgcctc cagcgctacg cgatcgagtt cctcccgtgt tttgcggtct
    13681 cgatgcccga gaaggaggaa catggtttgg gatcaatgcc aaaggccttg tggtggcttt
    13741 gacgaatcta tcggttcgcc ctcctgaccg tagcctccga tcccgagggc tgctgtgcat
    13801 ggatttgctt gcgctcgagg acgcgatgtc cgccgaacgc gcgcttcgtc atctcgaagc
    13861 caatagctac aacccgttca acatcgtcgc tatcgatacg accaccgccg ttcgcgcggt
    13921 ctacgacagt cgcccagatg ttcaacgcat gcctccaggc atctatgcca tcaccaactg
    13981 gcctttggat tcccctcatg attccaaacg tgaggggctt gaaactcgcg tgcgtagtcg
    14041 actctccccc caagccaccg cagccgaggt cattgatctc ctccaaaatg aagcccgcat
    14101 gcacctggaa gatgctgacc cccggacgag cgtatgctgc cactccccgc tgtatggtac
    14161 gagagcatcg tcaattgtcc tgctttctga cacacatgtg cgtatgcaac acgccgatgg
    14221 ccctccttgc tccacaccgt atgaaaacct atccgcctcc cttgctcgga tgatgagttt
    14281 caaaatattc tgatacaatt gacgctttca gggagcctgt cctaggcatg cccgcgaggt
    14341 aatgaacgtg gccgccatcg attgtcccaa gcgctcgtta attctcctac tcaccgcgct
    14401 agcctgttct gacgcgactt ccgatgatgc ctccaaacct tcgagccctc ccgaggcgga
    14461 tgctgcgggg gtgtcgttgc cttgtaccga caccgccgat cctctcgacc tcgacggctt
    14521 gtttgccctt tcggctcgcc tgaatttttc gttcgcaagt caaccggggg gagccgtgac
    14581 ggtttgtccg acggatcaaa ccagcgatgg tcatttcctt ggtttcgttc gggtgcagca
    14641 tgtggccgga caaaccgagg taccgtcggt ttcggcagtg atttgttcca tcgaccttcc
    14701 ggtcatctcg gcgattgtgg gagagtgtgt tcctcaagct tcgaatgtcg tgtacgccgg
    14761 cctcgagttc cccaaacctc tgatcgaaag ccttcccgtc gcttcttcgg cgcttaccac
    14821 ggcccagctc tcttcgccga atcctggcgc caccctttct gcgggctcga tgaacttttc
    14881 tatcggtacc aatgagcgta tcgcctcggc ccccggctgg ctcatgacgc attcgggctg
    14941 tggcatgaac gacgtcgccc ctggccgttc catggaatgt gagcagactt gcgtgaccga
    15001 ttgctcg
//
LOCUS       DAOSOR010000060        14969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4016_length_14969_cov_4.986389, whole genome shotgun sequence.
ACCESSION   DAOSOR010000060 DAOSOR010000000
VERSION     DAOSOR010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 14969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14969
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4016_length_14969_cov_4.986389"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PK710_05070"
     CDS             <1..763
                     /locus_tag="PK710_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09118.1"
                     /translation="RSRAVVDGGALAVSTCMATTGFSAAAALTCLGWSLTALGQETPS
                     TTTQLASATPYTVAEFGVGILALPTTDICLAQPNTCTKGDLTPHGWIWMLYRASSEFV
                     VGAGTSLARPFSGETTKRTEQIEREHIRQYMMIDVTGRYYGLRLPSVEGWLGMTFGGA
                     VISDQYKTTGEASDAVILGPAGVVVRTEGLSAGIAAGLGWAFAPNWTLESSFRSAWWF
                     LPSDRACGTTVDCATLSDDVAIFSLGIGVGYRISL"
     gene            complement(851..1294)
                     /locus_tag="PK710_05075"
     CDS             complement(851..1294)
                     /locus_tag="PK710_05075"
                     /inference="COORDINATES: protein motif:HMM:NF021983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2452 domain-containing protein"
                     /protein_id="HOT09119.1"
                     /translation="MAPTLAPSRSVPLNLVDIAAQIQKADQVLGCVTTQKLRIIVEQI
                     QALQQKARELLEQAQQSSDLHRVECRFQKRPGHVYHLYRRSEGSLYFSMLSPHDWRDH
                     PPDTFVGSFRLEQDMSFTPVQDIARRDAQDDMLRQLLPPSENGQR"
     gene            1483..3105
                     /locus_tag="PK710_05080"
     CDS             1483..3105
                     /locus_tag="PK710_05080"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT09120.1"
                     /translation="MQTLNELHVGRFLGRYELLMPVAQGGMASVWAARLKGTRGFQRV
                     VAIKTILPSLSDDARFEQMFLDEAALAARIRHPNVVQVLDLGEESGLLYQVMEWIDGE
                     PLSAFLRELPEGSGLPFPIAARILIGASEGLHAAHETCDDAGYSVELVHRDISPQNIL
                     VTRDGVPKVVDFGVAKASTLSSAATGTGTLKGKPAYMAPEQIQGAEIDRRADIFSLGV
                     MLYVLTTSRHPFRGKNDMATLHSIVSTEEAPAPSTFVDGYPEALEQVVMMAIAKDRER
                     RFKTASALARALEAAVPDVHKVGDAEVAAFTRTLLAERLEARAAALRSSLSDADLRAA
                     DSTSPPSHERMKNPSTLQGLGGPVPPPYEPSSSSSESVTRHEGRSPGTTSTVPPAKSR
                     STLAVTLVALGVAGLVFLAVAVAAILFWVRPEYLAKPTIPSTPSVDPSSVASPLSPSP
                     SGTGSSPAEADSNSNISVDSLPAATDLAPPSHPRPKGTSEPKVDATHPTPTDTPQPTT
                     VTTTPSVTPSTPTATPGTPTPGTVPKIRDPGF"
     gene            complement(3118..5076)
                     /locus_tag="PK710_05085"
     CDS             complement(3118..5076)
                     /locus_tag="PK710_05085"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOT09121.1"
                     /translation="MNLGLRVLAAALRAEGHQAVLFPFGSPHEFERVAQAIVMDRPDA
                     VGISINHEYSTLLSGALMKRLRELGFEKLIVAGGLVATLKAKELLTVCTELDAVVRHR
                     GEQAIVIVAEAAIGRRDLREAPGILLRQDDSFAQGAPCDVTGRSLWPWRGTEGLPKRL
                     GLPVASMMGSRGCARSCPFCSIVTLRQNAENLIPKTEALSVDSVPCHRRSAKDIASEM
                     AALVFEYGARVFEFQDDSFLPDDTQQAVTFLQDLRSHMQMHALPSCAIRISMHQDQVT
                     REVCNELANLGVIRATVDIKAASRSLAQRLHSDYSPSLARVVLGRLRRLGIATSFQSL
                     IIGPETTLEEIEDELEACSTIRGVPFELTRLHVFGGTILEQRLHDQKRLRDCFYFRKI
                     DFADSRVAQLDEWMSLLKSRYVSARSPIRRIIDLGYDIAIANRFFPHAGLFSLESLAA
                     RLVSRTNADQIHTARALIHLIRRGHTHENDIEPILRTAIRQDEALSHTIHDVAEILEE
                     EIQASGGQSPWADDFEPSELSLRLAELDADTAIMERPTMNAANAENRCAEIASRPCLG
                     PIRHSEAEEEEPLFSRTLPRIAPASMRAIIEVSNTGRARLVELADAQGIVDTTDTAHR
                     CVDEHLERFDMTCFAGERLIFEDPYPTT"
     gene            complement(5201..6442)
                     /locus_tag="PK710_05090"
     CDS             complement(5201..6442)
                     /locus_tag="PK710_05090"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOT09122.1"
                     /translation="MPDSHPSPCPQSPDDLNQIVLSPQDLAFERLVTKAILQSLQIDK
                     ILYVVLSGVTSGEGLGFNRGLFLLADDGQRSLRTGMAVGAIHREEAFRIWEEMVAKDL
                     TLEALLSVYDSVKDDPDAHQLSRRLGYLHLSLENLSEHASMAPDLSTIGRCRIETMLA
                     QSLLERRPLHSQTLELACEQISDSEPFSFVNWWIVPLLTSERIVGCLVVDHAFSDGEV
                     SVGRQHLLATIADLSAIAIEKSKIFDEMRALAQVDGLTGLANRHTYNQAIHRLLTQGR
                     QTGRPLSIIVLDIDHFKRFNDKHGHLAGDDALRFVASVLRANARKTDIVARYGGEEFV
                     VVLPDTTYEQALSVAEKLISTVRVESTTTAWGTITICAGVASSPEGGMDGTTLFENAD
                     KALYRAKRNGRDRVEGHFRIP"
     gene            complement(6576..8474)
                     /gene="aspS"
                     /locus_tag="PK710_05095"
     CDS             complement(6576..8474)
                     /gene="aspS"
                     /locus_tag="PK710_05095"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOT09123.1"
                     /translation="MAQRKFIDDWKRTHSCGDLRGADVGKQVVLFGWVQARRNLGGCV
                     FVDLRDREGLTQVVFDPSTEPRDLSVDGSETIAQAVAIAEQIRPEWVIGIRGVVVSRG
                     ENLNRNRPTGEIEVRVLEAEIFNQAETPPFEIVDDLDTREELRLQYRYLDLRRRPMQQ
                     IFRLRHQMNQTARRYLSDQGCLELETPFMVKYTPGGARNFLVPSRHAAGKFYALAESP
                     QLFKQLFMVAGFDRYFQIVKCFRDEDFRLDRQPEFTQVDIELSFVNQDDVFALVEGLM
                     FEIVQQTRGIDLREKYPEGRFPRMTFEESMQRFGNDKPDLRFGMEHTDITDLIIAHQG
                     GGVPFFAEIANRFENGTLRRDIPGEIVKVMSVSAEAKLSRKDLDEMEKMAQSMGAKGL
                     ARAKVGANGEWTQTALKTATVELRQAIHERTGAKEGDLLLFQFGKAALAHTVLANLRL
                     HLGKKMGHIPAFGHGGKLEFLWVVNPPMFEADEQTGGWVAAHHAFTRPHDDHIAIVES
                     DPAKVLCYRYDLVLNGFEVGGGSIRLHDPVVQRAVFRTLGIDEEQAEEKFGFLLKALR
                     HGAPPHGGMAIGWDRVAMLLSETESLRDVIPFPKTQRGADPMTDAPSALDDEQLAELR
                     LCVMPQKN"
     gene            8756..10510
                     /locus_tag="PK710_05100"
     CDS             8756..10510
                     /locus_tag="PK710_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09124.1"
                     /translation="MFNRTWISIAGIVSLLASCATFPPPEPHKPEADPDLLAGDDMET
                     TAEWLFVTSEADGKGQSECDRVSRWLQGEQSCTSDICIHARDLGREWLRKCKDESSAG
                     ATTVRKLVDTYAERAELPADSCVQQGTGLLRTPECGAPEACETQAQRWIAQCGTAYAT
                     PLFVLMLTKTLQRRFPDDPNKPVHEVKLDTRSCDELAKAVGQGVGCDGAACDPFVEVS
                     DAWLDRCRKDGQPVPMLLAFQLADVRVGAGRSVEPMRVAETKLAEGSLPLLLSDQRGA
                     VAWVCGVRPKNVKEYLEARRDCRPGEVIVTRVDGQQNVRTASVPHSDDAAFLRQFPFL
                     DVKGERDARALADMDAFRRDVSQAVEQAQGPHPEQAISLLVKVMQSRSEALMRQAVFQ
                     KILTDADRDLAPSFKEWGKRKAQGVVRVRGADEQGLYARRALQNPLHDMTRDGQVSAG
                     AYLAPPALTLDRWMPLSFLAYKDELSTLQRIVDRHGTLDNRVIPLRQQIASEMQACSQ
                     AEARIQSINDEIMACMLREGCTQDKIAALAFTADPDRSRAQRARDAIARALASGLFNR
                     GEMDKVEADRIASGCLDP"
     gene            complement(10534..10953)
                     /locus_tag="PK710_05105"
     CDS             complement(10534..10953)
                     /locus_tag="PK710_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09125.1"
                     /translation="MACSPHPPPASSSAPPSHPAPTTSPERQPNPPVSIPEEQTPSPT
                     PSKPVAKVLEILAGRDDPNDRRIRIEVSNPTDKPCTVKGYTVSWGSSKKSMPLADTVI
                     PPGESRQRFLIVHPRDGRVETLTVDGATVDMQYECDR"
     gene            11178..11492
                     /locus_tag="PK710_05110"
     CDS             11178..11492
                     /locus_tag="PK710_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09126.1"
                     /translation="MGARALAWGMGVIVALGCGEKVDCNRLCDREAECVAEIAVALGT
                     ATVEQTALIKDEDRKALGKKQKDRCMASCQSPTKPSSVHTKWRGCLQAESCEAFARCV
                     YR"
     gene            12139..14466
                     /locus_tag="PK710_05115"
     CDS             12139..14466
                     /locus_tag="PK710_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09127.1"
                     /translation="MIDPPLRRPPTSPPMGSTIFVVGEDDPALAEALRRGGNHVQWVE
                     SRDVLTRLEQQAPSLVIFDLPNKVEQAAVAELMGSLRAMGIGLFLIVGSESDENESTH
                     LSEDAQEQTGIESFDPASLRELSEEMVSRPFDLEQVADRISSLAQYRHGMRVAPPPPS
                     SRRPESVGVLDGMGEIASPKEPPAALLSYPLPNVDTSNPPGISSAPPGNEWSSIPAGP
                     LGLSLSFEAFAGPTSVSDELEKMLAEAERRVRSEARSTSEVPSPEAEVNAILPPEVLA
                     ALDEPLEVSDGELSEVESHTPVGQGTMGGAARVPETASKQREGMDLGDLSSPAEPVTA
                     HGEEDDWDERPRPHEAEYYDESASEDEGHEQRLQAMDVTPIPSRIGEPLPPMSTQMGT
                     SARQDRSFSQPAREVVLKPKVPSEPVPTPRVNWSVDLPSVLSPAFDGMRALGMCIAGR
                     ISATLSFEYQGALCQAVMRDGDFVTCGSSADDETLLAFLVLRGDLPKIAGTQLAGRFP
                     PFGRHAAAALIANGYVGQDQLWGVLRAHAEWLLGRMASTQGGTCALETEPQGRLRAEP
                     AVFGGATGAEVLVEVSQRVLPWEDALARIGGREARLGPGPHHSLLSECALSEKERDLM
                     ENAPGKTVREVLETARRPEFAAVIAVLKELGIIEIMPAIARSVHPEPDAFDPLDAEAV
                     RKRVAARLALIQEGDYFEVLGVPRTATAYEIRRAYLEARRAFEPSRLLTAQTIDLAED
                     AQLIVEVLDEAFELLRDEVRRERYRRAIEAVPPRR"
     gene            14633..>14969
                     /locus_tag="PK710_05120"
     CDS             14633..>14969
                     /locus_tag="PK710_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09128.1"
                     /translation="MASRYCWFVRHWFGSLLIAVSLLLVLTHGSNAHAQQRTFYLDRL
                     QIGGSPEDGFALWRPRMKQETVFFGQMALGWQHRPLRGATATYSERLANHTPNPVQNQ
                     FTTYLTAGLE"
BASE COUNT         3085 a         3877 c         4599 g         3408 t
ORIGIN      
        1 ccgttcccgc gccgtcgtcg acgggggggc gttggctgtg agcacttgca tggcgaccac
       61 gggattttcg gcggcggcag ctctgacgtg cttgggttgg tccctgactg ctctgggtca
      121 agagacgcct tcgacaacca cgcaactcgc atcggctact ccgtatacgg tggcggagtt
      181 tggggtgggt attttggcgt tgcccacgac agatatttgt ctggcccaac caaatacttg
      241 cacgaagggg gatctaaccc ctcatggttg gatatggatg ctgtatcgag cgtcgagtga
      301 attcgtggtt ggcgcgggca cgagtttggc gcgtccattt tccggagaaa cgacgaaacg
      361 gacggagcag atcgagcgag agcacattcg gcaatacatg atgattgacg tcacgggtcg
      421 gtattatggg ttgaggcttc cgagtgtgga gggatggttg gggatgacgt ttgggggggc
      481 ggtcatcagc gatcagtaca agacgacggg ggaggcatcg gatgcggtga tccttggccc
      541 tgcgggggtc gtggttcgaa cggagggatt gtcggcggga atagcggctg gattggggtg
      601 ggcatttgcg ccgaattgga cgttggagtc ttcttttcgt tctgcctggt ggtttttgcc
      661 gagcgatagg gcctgtggga cgacggtgga ttgtgcgact ttgtcggatg atgtggctat
      721 attcagccta ggcattgggg tgggctaccg catatcgttg tagacaccag ggtccgtcct
      781 tcgcttttcg tcgttgttta gcgctgtttt tgcgcctcct ggaggcaagg ggccatcccg
      841 tcgggggcag ttaccgttgc ccattttcgc tgggggggag aagttggcga agcatatcgt
      901 cttgtgcgtc gcgccttgcg atgtcctgga ccggggtaaa ggacatatct tgttcgaggc
      961 gaaaagagcc gacgaaggtg tcggggggat ggtcccgcca atcatgaggg gagagcatgg
     1021 agaagtacag ggagccttcg gagcgacggt acaggtggta gacatggccg ggtctttttt
     1081 ggaatcggca ttccacgcgg tgcaagtcac tggactgttg tgcttgttcg aggagttccc
     1141 gagccttttg ttgcagggct tggatttgct cgacgatgat gcgaagtttt tgggtcgtca
     1201 cgcagcctag gacttggtct gctttctgga tttgggctgc gatatcgacc aagttgagtg
     1261 gaacggagcg acttggtgct agggttgggg ccatgggcgg ggagggggaa gagcgatcgt
     1321 tgttcatgac ctgaatacgc gtaagcctgg atttggcatg catccttgct cacgtcaaaa
     1381 cgcatgtcca ttcagcaaga tgttggcgta tggtcgtggg gaaggataaa gacgattcga
     1441 cgtatctgaa cccgcaggga tacgcatagc caatcgacgc ggatgcaaac cttaaatgag
     1501 cttcatgttg gccggttttt gggccgttac gagctactga tgccggtggc tcaggggggt
     1561 atggcttctg tctgggctgc gaggctcaag ggtactcggg gatttcaacg agtcgtagcc
     1621 atcaagacca ttctgccttc tctttcggat gatgctcgtt tcgaacagat gtttttggac
     1681 gaagccgcgc ttgccgcgcg aatccgtcac ccgaacgtgg tgcaagtgct tgatttaggt
     1741 gaggaaagcg gtcttcttta ccaggtgatg gaatggatcg acggcgaacc gttgagcgca
     1801 ttccttcgcg aactaccgga gggaagcggg ttaccctttc ccattgccgc gcgtatcctc
     1861 attggtgcca gcgaaggatt gcacgctgcc cacgaaacgt gcgacgacgc tggttattct
     1921 gtcgaactcg ttcatcgcga catttctccc caaaatattc tggtcacgcg tgatggggta
     1981 cccaaagtcg tcgactttgg cgtggccaaa gcaagcactt tgtcgtcggc tgccacgggt
     2041 acagggacct tgaagggcaa gcccgcctat atggcgcctg aacagattca gggggcggaa
     2101 atcgatcgac gcgccgatat tttttcgctt ggcgtcatgc tttatgtact gacgacatcg
     2161 cgccatccgt ttcggggaaa aaacgacatg gcgacgttgc atagcatcgt atccacggag
     2221 gaagctccgg ctccatcgac gttcgtggac gggtatcccg aggctctcga acaagtggtg
     2281 atgatggcga tcgccaagga tcgtgagcga cgattcaaga ctgcttcggc gttggcgcgt
     2341 gctttggaag cagccgtgcc tgacgttcat aaggtaggtg atgcggaggt ggcagcgttt
     2401 acgcgtacgc tgcttgcaga aaggcttgaa gcgcgtgccg cggcgttacg ctcttcgctg
     2461 tccgacgccg atcttcgagc cgcggatagt acgtcgccgc catcgcatga acgcatgaaa
     2521 aacccttcga ccttgcaagg gttgggaggg cccgttccgc cgccgtatga gccgtcttcg
     2581 tcttcctcgg aatcggttac ccgtcacgaa ggacgttctc ccggcaccac cagtacggtg
     2641 cctcctgcca agagtcggtc cacactcgcg gttaccctgg ttgcattggg agtggccggt
     2701 ttggtctttc ttgctgtcgc cgtggcagcc atcctgtttt gggttcgacc cgaatacctt
     2761 gccaaaccaa cgataccttc cacgccctct gtcgatccct cttcggttgc ttcgccactg
     2821 tctccctccc cgagcggtac tggctcttcc ccggcagagg cggatagcaa ctcgaatatt
     2881 tccgtcgatt cccttccggc tgccaccgac cttgctcccc cttctcatcc aaggccgaaa
     2941 ggtacatcgg aacccaaagt cgatgcgaca catccaacgc ctacggatac gccgcaacca
     3001 acgacggtca ccacgacgcc atcggtgact ccgagtactc caacggcaac gccaggcaca
     3061 cccacgccgg gaaccgttcc caaaatccgt gatcccgggt tctaaaagcc catatcgtca
     3121 cgtcgtcgga tagggatctt cgaagatcag acgctcgcca gcgaagcaag tcatatcgaa
     3181 acgctcgagg tgttcgtcga cacatcgatg ggcggtatcg gttgtgtcaa cgatcccctg
     3241 ggcatcggca agttccacga gacgtgctcg tccggtgttg gatacttcga tgatggctct
     3301 catggacgcg ggcgcgatac gcggaagtgt gcgagaaaaa agcggttctt cctcctctgc
     3361 ctcggaatga cgaatcggtc ccaggcaggg tctggatgcg atttccgcgc aacggttttc
     3421 ggcattcgcg gcgttcatcg tggggcgttc catgatggcg gtatccgcat ccaattcggc
     3481 aaggcgcaaa gaaagctcgg atggttcgaa atcgtcggcc catgggcttt gtccccccga
     3541 ggcttggatt tcttcttcga gtatttcggc tacgtcgtgg atggtgtggg aaagtgcttc
     3601 atcctgtcga atcgcagttc tgaggatggg ttcgatgtcg ttttcgtggg tgtggccgcg
     3661 acgaatcaga tggatgaggg cgcgagcggt atgaatttga tccgcgttgg ttctggatac
     3721 caagcgtgcg gcaaggcttt cgagggaaaa taggccagca tggggaaaga agcggttggc
     3781 aatcgcaatg tcataaccaa gatcgatgat ccgtcgaatc ggtgaacgcg cgctcacata
     3841 ccgggacttc aaaagcgaca tccactcatc caattgcgcc acacggctgt cggcaaaatc
     3901 gatttttcga aaatagaagc aatcgcgcag gcgtttctgg tcgtgaaggc gttgttccag
     3961 aatggttccg ccgaaaacat ggagacgagt caactcgaaa ggaacgccgc gaatggtcga
     4021 gcacgcttcc aactcgtctt cgatttcctc gagcgtggtt tcgggtccga taatcaggct
     4081 ttgaaagctc gtggcgattc caagtcgtcg caatcgtccg agcaccaccc gagccaacga
     4141 tggtgaataa tcgctatgta gacgttgagc aagggaacga gaagcggctt tgatatcgac
     4201 ggtggcccga atgaccccga ggttggcgag ttcgttgcaa acctcacgag ttacttggtc
     4261 ttgatgcatg ctgatgcgaa tggcgcagct tggcaaagcg tgcatttgca tgtgagagcg
     4321 taggtcttgc aggaaggtca cggcttgctg cgtgtcgtcg ggaagaaatg aatcgtcttg
     4381 aaattcgaat acgcgcgcac cgtattcgaa gaccaaagca gccatttcgc tggcgatatc
     4441 ctttgccgag cgacgatgac agggcacgga atcgacgctg agcgcttctg ttttcggaat
     4501 caggttttcg gcattttgac gcagggtgac gatagagcag aaggggcatg agcgcgcgca
     4561 tcctcgactt cccatcatgc ttgccacggg aagcccgaga cgtttgggca atccttcggt
     4621 gccgcgccaa ggccataggg atcgtccggt cacgtcgcaa ggggctcctt gcgcaaacga
     4681 gtcgtcttgc cgcaacagaa taccgggagc ttcgcgcaga tcgcgacgac cgatggctgc
     4741 ttctgccacg atgacgatgg cttgttctcc gcgatgtcga acgacggcat cgagttcggt
     4801 gcagacggtg agcagttctt ttgctttgag ggtcgcgacc aaacctccgg ccacgatgag
     4861 tttttcgaag ccgagttctc gcaatcgttt catcagagcg ccggacagca gggttgagta
     4921 ttcgtgattg atgctgatgc ccaccgcatc gggccgatcc atcacgatcg cttgtgccac
     4981 gcgttcgaac tcgtgtgggg aaccaaaggg aaacaggacc gcttgatgtc cttcggcgcg
     5041 aagcgcggcg gcaagcacac gaagacccag gttcaccgaa ctctcggact cgggacccac
     5101 cagtgcaaca cgggctgata gcggtgcact gcgcatgaac cccatagatt aacaggttcg
     5161 acggatcagg aaagaccaga ttgcccgaag cgattttcgg ctatgggatg cgaaaatggc
     5221 cttcgactcg atctcgacca ttgcgcttgg ctcgatagag cgctttatcc gcattttcga
     5281 ataacgtggt gccatccatt cccccctccg gcgaacttgc aaccccagcg catatcgtga
     5341 tcgttcccca tgccgtggtg gtgctttcga cacgtaccgt cgatatcagt ttttcggcca
     5401 ccgatagcgc ctgttcatac gttgtgtccg gcaacacaac cacgaattct tcccccccat
     5461 accgagccac gatatcggtt ttcctcgcat tcgcgcgaag cacgctcgcc acgaaccgca
     5521 gggcatcatc tcccgccaga tgaccatgct tgtcgttgaa tcgtttgaag tgatcgatgt
     5581 cgagtacgat aatcgataag ggtcgtccgg tttggcgtcc ctgcgtgagc agacgatgaa
     5641 tggcctgatt gtaggtgtgg cgattggcaa ggcccgtcag cccatcgacc tgcgccaggg
     5701 ctcgcatctc atcgaaaatc ttactttttt cgatggcgat ggcagataaa tccgcgatgg
     5761 tggcgagaag atgttgtcta cctacggaca cctcaccatc gctgaaggca tggtcgacga
     5821 cgaggcatcc gacgattcgt tcggatgtga gaagagggac gatccaccag ttgacgaaag
     5881 agaaaggctc cgaatcggat atctgctcac aggcgagttc gagtgtttga gaatgcagtg
     5941 gtcgtcgttc gagcaagctt tgcgcaagca tcgtctcgat tcgacaccgt ccgatggtgg
     6001 aaaggtcagg ggccatggac gcatgttcgc tcagattttc gagcgataga tgcagatagc
     6061 cgagtcgtcg ggaaagctgg tgggcatcgg gatcgtcttt gacggaatca tagacggaca
     6121 gcagcgcttc gagcgtgaga tctttggcca ccatctcttc ccagattcga aacgcttctt
     6181 cacgatggat ggcaccgacg gccataccgg ttcgaaggct gcgctgcccg tcgtcggcaa
     6241 gaagaaaaag acctcggttg aaacccaatc cctcccctga ggtgactcca gacagcacga
     6301 cgtagaggat tttgtcgatt tgcaaggatt gcaggatggc tttggtgacg agccgttcga
     6361 acgccaggtc ttggggagaa aggacaatct gattgagatc gtcgggagac tgagggcacg
     6421 gagagggatg gctatcaggc attcgaacgc caattcatga gcagagcttg ggcacgtcag
     6481 gtgtgccgat caccgtatga cgagcggtta tgacgactcg tcgtatcgag ttcccattcc
     6541 aacaagtatc gatgagtgcg gttccgcgag tagtatcaat ttttttgagg catgacgcaa
     6601 aggcgcaatt ccgcgagttg ttcatcatcc aacgccgaag gtgcatcggt catggggtcc
     6661 gcgccgcgtt gggttttggg gaaaggaatc acatcgcgca gactttctgt ctcggacagc
     6721 aacatggcca cgcgatccca accgatggcc atgccaccgt gagggggagc gccgtgtcgt
     6781 agggctttga gcaaaaaccc gaacttttcc tcggcttgct cttcatcaat cccaagagtt
     6841 cggaatacgg ctcgttgcac cacgggatcg tgaagacgaa tcgagccgcc gcccacctcg
     6901 aagccgttga gcaccaagtc ataacgatag cacagcactt tggcgggatc ggactcgacg
     6961 atggcgatgt gatcgtcgtg ggggcgcgta aaggcatgat gtgcggcgac ccagccgccg
     7021 gtttgttcat ccgcttcgaa catgggagga ttgacgaccc acaagaattc gagcttaccg
     7081 ccgtggccga acgcggggat atgtcccatt tttttgccaa ggtggagccg gagattggcc
     7141 agcaccgtat gagcaagcgc cgcttttccg aattggaata gcagcaggtc accttccttg
     7201 gcccccgttc gttcgtggat ggcttggcgt aattccacgg tggcggtttt caaagcggtc
     7261 tgtgtccact ccccattcgc gccgactttg gctctcgcca atcctttggc tcccatcgat
     7321 tgcgccatct tttccatctc gtcgaggtct tttcgggaca gctttgcctc ggcagagacg
     7381 ctcatgactt tgacgatttc ccctggaata tcgcgacgaa gcgttccgtt ttcgaaacga
     7441 ttggcgatct cggcaaaaaa cggaacaccg cctccctgat gggcaatgat caagtccgtg
     7501 atatccgtat gctccatgcc gaaacgcagg tccggcttgt cattgccgaa ccgttgcatc
     7561 gactcctcga acgtcatccg aggaaagcgt ccttccggat acttctcgcg caaatcgatt
     7621 ccgcgcgtct gctgcacgat ctcgaacatc aatccttcca cgagcgcaaa cacgtcgtcc
     7681 tgattgacga aggacaattc gatatcgact tgtgtgaatt ccggttgtcg atcgagccga
     7741 aagtcttcat cgcggaaaca cttgacgatc tggaagtagc gatcgaatcc ggccaccatg
     7801 aaaagttgtt tgaatagttg cgggctttcc gccagggcgt agaacttacc ggccgcgtga
     7861 cgcgatggga ccagaaagtt gcgagcacca ccgggcgtgt acttgaccat gaacggcgtt
     7921 tccagttcga gacatccttg atcggacaga tatcggcgag ccgtttgatt catttggtga
     7981 cgcagccgaa atatctgttg catggggcga cgtcgcagat cgaggtatcg gtattggagg
     8041 cgaagttctt cgcgtgtgtc caagtcatcg acgatttcga agggtggggt ttcggcttga
     8101 ttgaatatct cggcttcgag gacgcgcact tcgatctcgc cggtgggtct atttcgattg
     8161 agattttctc ctcgggaaac gacgacgccg cggattccga tgacccattc cggtcgaatc
     8221 tgctctgcga ttgcgactgc ctgggcgatg gtttcggagc catccacact gaggtctcgc
     8281 ggttccgtcg agggatcgaa gaccacttgt gtcagacctt cccgatcgcg aagatcgacg
     8341 aatacgcatc cacccaagtt gcgccgtgct tgtacccagc caaatagcac cacctgctta
     8401 cccacgtccg caccgcgtaa gtcgccgcaa gaatgagttc gtttccaatc atcgatgaac
     8461 tttcgctgtg ccacgttcgt gctccttgaa cttgtcatgg ggggggtcag cgtagtgaag
     8521 gctcgcctga tgtccagcta cgtcgagggg gcagattcgt tgtttccgat tctttccatg
     8581 ctgccgtacc tggcttgccc ccatcgaaaa aatgcgatgg ctgaagcaat tcacacgatt
     8641 ttttcgaatc gctcccacga aggttgcgct aagacgggcg ccagttgtgg gcttggttcc
     8701 ctcgtatcgc aaatggaagt agggtcggac ccggttttgt ctaaggaacg ctgctatgtt
     8761 caatcgaact tggatttcca tcgctgggat cgtctcgctg ttggcgagtt gcgctacgtt
     8821 tcctccccct gagccgcaca aaccagaagc ggatccggat ttgctcgcgg gcgatgacat
     8881 ggaaacaacc gcggaatggc ttttcgtgac gtcggaagcc gacggtaagg ggcaaagcga
     8941 gtgcgaccgg gtaagccgtt ggttgcaggg tgagcagtcc tgcacgagcg atatctgcat
     9001 tcatgctcgg gacttgggcc gagaatggct acgaaaatgc aaagatgaat cctccgcggg
     9061 cgcgacgacc gtgcgaaaac tcgtggatac ctacgccgaa cgggctgaat tgccggcgga
     9121 ttcctgtgtt cagcaaggaa cgggattgct tcgtactcct gaatgtggag cgccagaggc
     9181 gtgtgaaaca caagctcagc gttggattgc acaatgcgga acggcttatg ccacgccgct
     9241 gttcgtgctg atgctgacga aaacgcttca acgtcgtttc cccgacgatc ccaacaagcc
     9301 cgtgcatgag gtgaagctcg atactcgatc ctgcgatgag cttgcgaaag cggtcgggca
     9361 aggagtgggg tgtgacggtg cggcatgcga tccgtttgtg gaggtcagcg atgcatggct
     9421 ggatcgatgc cgtaaggatg gacagccggt tccgatgctt cttgccttcc agttggcgga
     9481 tgtgcgcgtg ggagcgggac gtagcgtcga acccatgcgt gtagcggaaa caaagcttgc
     9541 ggaaggaagc ttgcctttgc tgttgtccga tcaacggggg gctgtcgcat gggtttgcgg
     9601 cgttcgcccc aaaaatgtga aggaatatct tgaagcacgt cgagattgcc gacctggtga
     9661 ggtcattgtc acgcgcgtgg atggccagca aaacgttcgc accgcttcgg tacctcactc
     9721 cgacgatgcc gcgttcttac ggcagtttcc ttttcttgat gtgaaaggcg agcgcgacgc
     9781 acgcgccttg gcggatatgg atgcttttcg ccgcgatgta tcccaagcgg tggagcaagc
     9841 ccaagggccg caccccgaac aagccatttc tctgctcgtc aaggtaatgc agtctcgatc
     9901 cgaggcgctg atgcgccagg cggtgttcca aaaaattctt acggatgcag atcgggacct
     9961 tgcgccttcc ttcaaagaat ggggaaagag aaaagcacaa ggtgtcgtca gggttcgcgg
    10021 ggccgacgaa caaggccttt atgcacgtcg agcgctgcag aaccctttgc atgacatgac
    10081 ccgtgacggc caagtgagcg ctggagcgta tctggctccg ccggcgctta ccctcgatcg
    10141 atggatgccc ttgtcgtttc ttgcctacaa agacgaactg tccacgcttc aacggatcgt
    10201 tgaccggcac gggaccctcg acaatcgagt gattcccctg cgtcagcaga tcgcgtcgga
    10261 gatgcaagct tgctcccaag ccgaagctag aatccagtcg atcaacgatg aaatcatggc
    10321 ttgcatgctc agggaaggat gcacgcaaga caagattgct gctttggctt tcaccgcgga
    10381 tcccgaccga tctcgtgcgc agcgcgctcg ggatgcgatt gccagagcgt tggcaagcgg
    10441 attgttcaat cgtggagaga tggacaaagt cgaggcggat cgaatcgcca gtggttgctt
    10501 ggatccctaa ccaaacaaca ttctccgaac cgatcaacga tcacattcgt actgcatatc
    10561 caccgtggct ccatcgacag tcaacgtttc cactcgtcca tctctcgggt gaacgatgag
    10621 gaatcgctga cgactctccc caggtggaat gacagtgtcg gcgagaggca tggacttttt
    10681 cgaagagccc caagacacgg tatacccttt gaccgtacac ggtttgtcgg tggggttgga
    10741 tacttcgatg cgaatgcgac gatcgttggg atcgtcgcgg ccagcgagta tttcgagtac
    10801 tttggcgacg ggtttggatg gtgtgggtga gggagtctgc tcttcgggga tagatacggg
    10861 cgggtttggt tgcctttcgg ggctggtggt tggagcagga tgggatgggg gggctgagga
    10921 ggatgcaggt ggtggatgtg ggctacaggc catgatccct gcaaaaccga ggaggaatat
    10981 ggtggtccga gtcgaacagg cgtgacgcat ggcgtgccat tatcgaatcc gaacgttcga
    11041 tcccacaacg ccgtttttgt ttcggatggg ttctttttca tcgatcggta gcttcacgac
    11101 atgatccgaa tcgtttttat gctcttcgtg ctacccttat tcccattgaa catatgacga
    11161 agcgtgggag gtcaccgatg ggtgctcgtg ctcttgcgtg ggggatgggg gtcatcgttg
    11221 cgctgggatg tggggaaaag gtggactgca accgattatg tgatcgggaa gccgagtgtg
    11281 tggcggagat tgccgtggcg ctggggacag cgaccgtgga gcagacggcg ttgatcaagg
    11341 acgaggatcg gaaagcgctt ggaaaaaagc aaaaagatcg atgcatggcg agctgccagt
    11401 cgcccaccaa accaagttcc gttcacacca agtggagggg atgtcttcaa gcggaatcgt
    11461 gtgaagcttt tgcgcggtgc gtgtaccgct gacgagcagt atggttcgaa ggccgaggtg
    11521 ggttccgcac atcatgggcg tttcgaagca atcgaccacg tggcagatgg tgaatgctgc
    11581 cacaacccac gacgatgggc ttgccgtggc gcactctcac gctccaagaa aaaatgagtt
    11641 ttcggtgacg aatgctgtgg tgtgtgtttt gcgtgaccgt gtatccatgg cagtggagca
    11701 tcgggcaaga ggcaagccga gcgtgggcag gtattcggaa aaagtgattt gggtggttgt
    11761 catggttggt ggagccgcga gcgattccag agcaatacct atgatatgat ttgtgtaaca
    11821 tgttgaatgt attggtaaat attggtgcat gtcgaaacca cgaccggttt gcctcggccg
    11881 tggtcgaggg aagatgactt tcggggcata agttgtgata gcaatgcctt gtgttagcca
    11941 tgacttggaa tgagatcgct ctggtggtct tcctgttcgt gctagtcacc tcttgggggg
    12001 tgttgcagcg tttgggcgat tggattggag tgttgcttgg gccgaaacga tcttcgaagc
    12061 cgcgtggttg acgaatgaat ttggttgaat gggcatacac caccgcccgg tcttttcgtt
    12121 ctggtaaact tggttgtcgt gattgatcca cctcttcgcc gtcctcctac gagccctccg
    12181 atgggcagca cgattttcgt ggtgggggaa gatgatcccg cgcttgcgga agcgttgcgc
    12241 aggggaggaa atcatgtgca atgggtggag tcgcgcgatg tgctcacgcg gttggaacag
    12301 caagctccta gtttggtgat cttcgatcta cccaacaagg ttgagcaagc cgcggtggct
    12361 gagctgatgg gttctcttcg ggccatgggg attggtctat ttcttatcgt tggctctgaa
    12421 agcgatgaga acgagtcgac gcatctatcc gaggatgcgc aagagcaaac aggaatcgaa
    12481 agcttcgacc ctgcgagtct gcgtgagctg tcggaggaga tggtttcgcg accattcgac
    12541 ttggaacagg ttgccgatcg gatttcgtcg ttggcacagt atcgacatgg aatgagggtt
    12601 gcccctccgc cgccatcgtc gcgacgtccc gaaagcgttg gcgttctcga tgggatggga
    12661 gagatagcga gtcccaagga gccccctgct gccttgttga gctatccctt gcccaatgtg
    12721 gatacgtcga atccgccagg gattagctca gcgccgccgg gtaacgaatg gtcgtcgata
    12781 cccgcgggac cgttgggatt gagcctttct ttcgaagcgt ttgcgggtcc cacgtccgta
    12841 tctgatgagt tggaaaagat gctggcggag gcggagagga gggtcagatc cgaagcgcgt
    12901 tcgacttcgg aagtgccgag tccagaagcg gaggtcaatg cgatcctacc tccggaggtt
    12961 cttgcggcgt tggatgagcc gctggaggta tccgacggtg agctatcgga agtggagtcc
    13021 catacgccgg tgggtcaagg tacgatgggt ggggctgcgc gagtgccgga aaccgcgtcc
    13081 aaacagcggg aggggatgga tctgggggac ttgtcctcgc ccgccgagcc tgtgactgcc
    13141 catggcgaag aggatgattg ggacgagcgg ccgaggcccc acgaagcgga gtattatgac
    13201 gagagtgctt cagaagacga agggcatgag caacggctgc aagcgatgga tgtcacacct
    13261 atcccttcga gaatcggcga gccgttgcca ccaatgtcca cgcagatggg aacgtctgcc
    13321 cgacaggacc gttcgttttc gcagcccgct cgggaagtcg tgctgaaacc gaaagtaccg
    13381 tcggagcctg ttcctacgcc tcgggtgaac tggtcggtcg atctgccttc cgtgctttcg
    13441 ccagccttcg atgggatgcg ggcgctgggg atgtgcatcg ccgggcgcat ctcggccaca
    13501 ttgagtttcg agtaccaagg ggcgttgtgc caagcggtga tgcgtgatgg ggatttcgta
    13561 acctgtggtt caagcgcgga cgacgagacg ttgttggctt ttttggtgct tcgaggggat
    13621 ttgccgaaaa tcgcgggaac ccagcttgcg ggaaggtttc cgccgtttgg gcgtcatgcg
    13681 gcggcggctc tcattgcgaa tggatacgta gggcaagatc aactttgggg ggtattgcgt
    13741 gcgcatgccg agtggttgct tgggcgaatg gcaagcacgc agggaggaac gtgcgcgctg
    13801 gaaaccgagc cgcaaggacg gcttcgagcc gagccagcgg tatttggcgg cgcgacaggc
    13861 gcggaagtgc ttgtggaggt atcgcaacgg gttcttcctt gggaagatgc cttggcgagg
    13921 atagggggac gtgaagcgcg tcttggtccg ggtccccatc attcgttgct ttcggaatgt
    13981 gctctttccg aaaaagaacg agatttgatg gaaaatgcgc cgggaaaaac ggttcgagag
    14041 gtgctcgaaa ccgcccgtcg ccccgaattt gctgcagtta tcgctgtctt aaaagaactt
    14101 ggaatcattg aaatcatgcc ggcgatcgct cgttccgttc accccgaacc ggatgctttc
    14161 gacccgctcg atgcagaggc tgtgcgcaaa cgcgtcgcag cacgtctcgc cctcattcaa
    14221 gaaggcgatt acttcgaagt gcttggtgta ccgcgcaccg ccaccgcgta tgagattcgt
    14281 cgcgcttacc tcgaagcgcg acgtgcgttc gaaccttcac ggctgttgac tgcgcaaacc
    14341 atcgatcttg ccgaagatgc acagctcatc gtcgaagtgc tcgatgaagc tttcgaactg
    14401 ttgcgggacg aagttcgaag agaacgctac cggcgagcca tcgaagccgt tcctccacgt
    14461 cgatagatat cgctcgaaag caccaaatcc atcgttcctt caccttgggg ctcgacgcgc
    14521 gccgcgctcc cctttaggct cttcgacgct cgaccaccct cgagcgaatt tcttgcatcg
    14581 gtattctcgt tcgtcccctg tcgattacag cccggtgtgg ggaggtcttc ggatggcatc
    14641 tcggtattgt tggttcgtac gacattggtt tggcagtctg ctcatcgcgg tctcgttgct
    14701 gctcgtgctc acccacggct cgaacgctca tgcgcaacaa cgaacgtttt atctcgatcg
    14761 cctgcaaatc ggaggctctc cagaagacgg cttcgcctta tggcgtccac gcatgaaaca
    14821 ggaaaccgtg ttctttggac aaatggcgct cggatggcag catcgacctt tgcgaggagc
    14881 cacggcaacg tatagcgaac gtctcgccaa tcacacgcca aaccccgtcc aaaaccaatt
    14941 cacgacctac cttacggccg gactcgaac
//
LOCUS       DAOSOR010000061        14918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4034_length_14918_cov_4.820965, whole genome shotgun sequence.
ACCESSION   DAOSOR010000061 DAOSOR010000000
VERSION     DAOSOR010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 14918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14918
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4034_length_14918_cov_4.820965"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="PK710_05125"
     CDS             <1..1219
                     /locus_tag="PK710_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOT09129.1"
                     /translation="MPDDEPIEHPWVTKSIENAQTKVETRNFDIRKNLLEYDDVMNAQ
                     RKTVYGIRQALLAGRYQPEQLDEIGKPTGKTRDIQPMQSIVDSVTPVVGQLIGLYLEQ
                     PVQPHGADGKPREITREQLAGGKVVEHGQLRHEIYHLWGIAVDVEGKKDPGDLYDEVA
                     ALIPQGLTEQRERALDLIDRIISAMVEESCPANRPPEDWDWQSIREGFEDHFGRKPDS
                     IEDLGDQEMLAGEMYRQAESAFIEKENDLGQELLLRVFRHIYLEEIDKAWVEHLTNMD
                     HLRDGIGLRGYGHRDPKQEYKREGYDMFVNMMAAVSANVVSKLFRVQVQRQEQAAQME
                     AEYAARKAREMLEAAARHGYGASEAPPPISAEPVAQKPKAKRIGPNDPCPCGSGKKFK
                     KCHGSFAEDSSDA"
     gene            1277..1681
                     /gene="ybeY"
                     /locus_tag="PK710_05130"
     CDS             1277..1681
                     /gene="ybeY"
                     /locus_tag="PK710_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOT09130.1"
                     /translation="MRTRARRMLDALGRLDSELSVVLVDDTIMQSLNATYRGKDQTTD
                     VLSFAMTEGDFGDVNPDVLGDVVISVPTARRQATQARQPLLDRVTFLLAHGLLHLIGY
                     DHETDEQHREMTRQTKRLMRKALDPKPITNKK"
     gene            complement(1665..2537)
                     /locus_tag="PK710_05135"
     CDS             complement(1665..2537)
                     /locus_tag="PK710_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOT09131.1"
                     /translation="MRISQFSYAMVVGLLTCGTLARAQSTTQECPPGAWFCEEAPDSA
                     PPQELPPPAPTPPPPAAENKQAPTTIVVPGAQASQPIVVYQQTPPPPPRVVLVRRPAA
                     PFPVPPPPPPKKRERYRPWGFNLHIAGVLMDNESNESTGMGGAGIALRLRPERFVAID
                     VGLDMLGGRDYQGYRRSELPFSLSALLYANPRDATQFYVLGGLDWSAAHVKIQERSNE
                     SVDYSYFGLHTGLGLEFRLSHPVALNLDLLGFIRTRTDSLASTNPEFTDPDTGQTTNS
                     SGGGLLRAGLTFYW"
     gene            2727..3917
                     /locus_tag="PK710_05140"
     CDS             2727..3917
                     /locus_tag="PK710_05140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOT09132.1"
                     /translation="MPSVILKPGHVQPVWSGHPWVYSQAVERVQGGATAGDEVTVLDP
                     RGNYLGSGYYSPRSAIVVRIATRNPKARLDNSWLRKRLSQAHELRHEMGLPTVRTNGF
                     RLVHAEGDNLPGLVVDRYNETVAIQLTTLGMHRRRAMVMDAIQGVFGVKTIVDRTPTS
                     FAKLEGFEPEPGIVCGPMDLDAYRFMERGFVYEVPMALTQKTGYYFDQRPLRARVEQL
                     SRGRRVLDAFCFVGSFALAAARGGATSVLAVDENVIALETGARCAKANGLSDRIAFSR
                     GSVRTLLSSSQHTAKFDLVVLDPPSMAPSQKSLQRASKAFRWLVAQGCRVLRPGGLMV
                     LSACSAAVDVNTLTRMLAMGARDAGMQAIVLERWFQGMDHPVPAAFPEGLYLKSLIAR
                     INPI"
     gene            4184..6424
                     /locus_tag="PK710_05145"
     CDS             4184..6424
                     /locus_tag="PK710_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOT09133.1"
                     /translation="MTQREQPLSRLPAGPMVAPVDCPGCARKIDPLRAGHVAIFANNF
                     HYFCDYSCRVHYLTQHPELVRPTPKTSLPPGPQALAQSLPTLDDAEPVAFDADTELLN
                     VDEQAAIGATPAVPKSDPGAILLVSATVTGVLAIALALTGTGLLALTIRLAMACLGVV
                     LLVIRSSLSPRTPSDPHPAALLSPAVAAAIIALWARMVGHEIADESAVLTGLIVVSTG
                     TSLQLIEQVRRGPMHRISTIQQVLDAPARRVTPSGYAIVASNQLRPGEELVIDAGEVV
                     PADVVISAGEATVLPWLGAQSATSRVAGQSLVAGSRVVTGRLRATVSWTGMDRAWLCT
                     TADPHRAAHVMAPVTRHARRLVERWALGAAAVSGLAAFSNGLSTPAVLLAAIAAHAAV
                     ASAATGATPSVHVLRGVLGALERGVAYRHALAWERAAQSTLAVFSARGTLLLGEPHVA
                     EIVSLGRMTTERLLSVAAGAEALASDAIALAIKRAALDRQVELDAVRSPHVVPGLGVT
                     AVTSAGEPLCVGSRALMMREHLSMASVENRLAELEAMGRTVLLVGIGGKLIGFIALQD
                     GLRPGARAAVQHLLDAGVEPVLMSGDARETCEAIARSLDIEHVRPEVLPSDRAQELKS
                     MTDSGATVAMVGRLGEDEAALSAADVAIVLEAAGSAVGDWSVTLASDDVRDAADGLVA
                     ARRARLHARAAMFLGLAPGVASALAIAFGLLPPAFAPLAVLVSAVGTHLHLRAVAPSQ
                     HDDTSG"
     gene            6405..9749
                     /locus_tag="PK710_05150"
     CDS             6405..9749
                     /locus_tag="PK710_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOT09134.1"
                     /translation="MTTRPVDANTAMARRARPPWRISHAVKGTELESAIGMVYCTRDI
                     LFRICFRAAVLILCLLSAQPSEAQETEDSLTPIHAASDLGDLIGKPIQQVQVVQTGRW
                     RSEALVTSVKIGEPMTGAAARRAAQELLNTGGFSDVQVFVERSGQGVRLRLEAVPRRL
                     IAARRLVGSTLDATELWQTMGLRDGDELTDEKLVQIQKQVQDIHRSRGYPRARVSVQT
                     RDTDARAKVVLIVQVDAGPPLMIADRIFIMPDATWSVLSDVAQRYRVSKADRLDEEAL
                     ASANVELEEDLRARRYPMARVRHSSVVKDGFGFVYVYVQAGPHLRFRFEGNERFDADQ
                     LTDVLDYDKQLDRTAAALTDRLASFYRRYGFLDVEIHAESRGTATDLVNELVFHIREG
                     EIVQVYGKEFPCLRGGPRSVDALLAEVDGVLTNELPGGSLIGSVDPKIVDATLGPKGV
                     TGARPSPLEPDPTRIYDPATYDKVVQHVRDLYRSEGFLSAMVGPVRVLRRTCHRHSPP
                     GQCVPLPLPSRPSSACQFDPRGLPVDDEAPDARLQCRPDRARAITCEPRVWLHIPVKL
                     GPRAMLYDVAFDGNRSIPEADLLEASELDVGQPASNLELEKARRRVIQAYREMGFAYA
                     EVRATLDLSPDHTRARARFIVNESQQVIVDKIIVVGAERTSEPLIIGRVSLKIGQPYR
                     ASDVRKTEERIATLGTFSSVAVSLQDAYVPARRKTVVIQVQERLPQYLDIRPGFSTGE
                     GVRIQFEYGHLNIAGEAIRFTLRLRLSYLPDVFILDEDVRNNLDTLPMSRRLERHNTA
                     SVLFPEIGLGPLVSLNFDGIDVRSNARDFGLSKNALATTLNIRPFRTLSFGLGGSLER
                     NDVAIFSDQSVIEYLQRPGVTGDLRRLLLAPDGLTLAVAQLATVSWDRRDNPLGATRG
                     TLLGGSVEHVRAFPGEDNPNTKLSDFLRVSGIVSAYLPLNDNGLTLAASMRAGRIIQM
                     MSSSDTYPDRLFFLGGVDSVRGFLRDSVVPQDVAERILQGSPQDPNRLTVNQVAIRGG
                     DVFLNPRLELRIPMTATVHTTLFMDSGNVWVEPEAFEPWKLRYASGSGLRIATPIGPL
                     AFDYGINLDRRSWEDFGAFHFSIGLF"
     gene            9931..10707
                     /locus_tag="PK710_05155"
     CDS             9931..10707
                     /locus_tag="PK710_05155"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOT09135.1"
                     /translation="MPTFEPFEHRQALAQQAKGVLRKQMRGVRATIPTAAMAQRSRQL
                     QTRIMQLPIWDRVRTVLLSESAHQKGVSTAWVAQQARSRGITVAYPRVDYDAQRVEYR
                     IATEADLEDRGLETAEAPKNAFLVPPGTIDLVIVPVLAVDPDGFFVDDGSGAYDRVLR
                     DLAQAMTLAVAFEFQLIPEAPRTSEDVPVAVIVTDRRVIEARNKRVESGSENTHHTNR
                     ALEEDRRAIQPNLMVGPIHRADGAGGGTGHRTGQCPQEGF"
     gene            10604..12307
                     /gene="rny"
                     /locus_tag="PK710_05160"
     CDS             10604..12307
                     /gene="rny"
                     /locus_tag="PK710_05160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOT09136.1"
                     /translation="MPFNQTLWLVLSIALTALVVGLAIVLGNARKKVFELQQQIRHKG
                     NEDEQIVRQAKEEAERILAQARLQGQREADTITLEAQTQLRVVEGKSQEVDARRKAAE
                     VEAREILLKARDAAEKEAQQAATAQQQALEDLEKRSQKRERVIREKEDLVASRELELT
                     RREKGLQTREQQLQSEEARIAEIIHAQEDKLQQIAGMSSEDAKNRLMERYKDDARRGA
                     ANDAKRIEDEAREEAEKKAKRIIGLAIQRFAGEYVQDRSVTSVHLPNDELKGRIIGRE
                     GRNIRALQEATGVDFIVDDTPETIVVSSFDPIRREVARIALEKLIVDGRIHPTRIEEV
                     VTKSQEEVERAMREAAEQAMLELSITRVHPEIVKLLGRLRYRYSYAQNVLRHSVECGF
                     LCGLMASELGLNVRQARRAGLFHDIGKAVSHEQEGGHAVIGGQIARKYGEDEVVANAI
                     ACHHDDEPCKHLLGHLVAAADTLSGARPGARREMLESYVQRLEDLERISTSFTGVERA
                     YAIQAGREVRVLVEPSEVSDAESALLAREIARKVEESLTYPGQIRVTVVRETRAIDYA
                     K"
     gene            complement(12320..13873)
                     /locus_tag="PK710_05165"
     CDS             complement(12320..13873)
                     /locus_tag="PK710_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOT09137.1"
                     /translation="MHLGIDFGTTRTVVAACDRGNYPIVSFASEDGDFADWYPSLVAE
                     RNGELRCGWAAHACASDPSWVVLRSFKRMLTEPTVRPDHVVKVGSLRMPLIDLLVTFL
                     RSLHNDLRSQSSMQAALHPLESIPVMIATPANAHGTQRFLTIDAFQKSGFQVLALLNE
                     PSAAGFEYAHRYRSTITSKREHVVVYDLGGGTFDASLVRMNGQNHDIVYTSGIPRLGG
                     DDFDTVLADLVLGEKGMMELSESDPRRERLVSVCREAKESLVQGSRRVLVDVESAWGE
                     HAPVREVALPVSTFYEACTPLIEQSIEAMMHVVERGEGGGDTRNLLGIAGIYVVGGAS
                     ALPAVGRTLKHKYGRRVHRSPYPSAATAIGLAIAGDKEAGYRLADRFSRVFGVFRELR
                     DGAVVSFDPVFTKDTCMPLSQERCVTSSRTYRAAHNIGHFRYVECDDIDSSGVPSGDI
                     TPFAEALFPFDPMLCEGKEDLRTIPVRRLASPGPLVEERYTVDADGIVEVSIRNLDAG
                     YEKKYRLWG"
     gene            complement(13910..14650)
                     /locus_tag="PK710_05170"
     CDS             complement(13910..14650)
                     /locus_tag="PK710_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOT09138.1"
                     /translation="MTREEIAEIQSRYGLGPNLAADLAVLTAIWWEEQVDLRLLLIQR
                     KNKPFQGQWALPGGFVDMDEDLETAARRELQEETGIGDLGSTPIEQLGAFGNPWRDPR
                     SRVVSAVFLAWVRHGDLPEPQGGDDAASAEFVSLRQGIPVDKQDKPLRLAFDHDVVLQ
                     KVWQRVRLQTAQSSIPLVLLPERFSTRQLVALYEALIGPSESQPLLTWLQHHDWIRPE
                     GSWWVRGDRVHHEARPAWTVPPLVPIVS"
BASE COUNT         3191 a         4034 c         4562 g         3131 t
ORIGIN      
        1 gatgcccgac gacgaaccca tcgaacatcc ttgggtcacc aagtccattg aaaacgcgca
       61 aacgaaagtc gagacccgaa acttcgacat tcgcaaaaat cttctcgagt acgacgatgt
      121 catgaatgcc cagcgcaaga cggtgtatgg gatacgtcag gcgcttctcg cgggaagata
      181 ccaacccgaa cagctcgatg agatcggtaa gcccactgga aaaacacgag acattcaacc
      241 gatgcaatcg atcgtcgact cggtcacgcc cgtggtgggt cagctcatcg gattatatct
      301 cgagcagccg gtgcaacccc acggggccga tggcaaacct cgtgagatca cgcgcgagca
      361 gctcgcgggg ggaaaggttg tcgaacacgg acagcttcgt catgagatct atcacttgtg
      421 gggaatcgcc gtggatgtgg aaggcaaaaa agatccgggc gatctgtatg atgaagtcgc
      481 cgcgttgatt ccccaaggac tcaccgaaca gcgagagcgc gcactcgatc tcatcgatcg
      541 catcatcagc gcgatggtcg aggaatcctg tcccgccaat cgtcctccgg aagactggga
      601 ttggcaaagc atccgcgaag gattcgaaga tcacttcggc cgcaaaccgg attcgatcga
      661 agacttaggt gatcaagaaa tgttggccgg ggagatgtat cgtcaggcag aaagcgcctt
      721 catcgaaaag gaaaatgacc taggacaaga gctgttgttg cgcgtatttc gccatatcta
      781 cttggaggaa atcgacaagg cttgggtcga gcacttgacg aacatggatc atcttcgcga
      841 cggcatcggg ttgcgcggtt atggtcatcg cgaccccaaa caagaataca aacgagaagg
      901 gtacgatatg ttcgtcaaca tgatggcggc ggtctctgcc aacgtcgtat ccaaactttt
      961 ccgggtccag gtgcagcgtc aagaacaagc cgcgcaaatg gaagccgagt acgccgcgcg
     1021 caaagcccgc gaaatgttgg aggccgccgc gcgtcatggc tacggtgcct ccgaagcacc
     1081 acctcccatt tcggccgagc ccgtggcgca aaaaccaaaa gcaaaacgaa tcggacccaa
     1141 cgatccatgc ccctgtggct caggaaagaa attcaaaaaa tgtcacggct cgttcgccga
     1201 agacagttct gacgcttgat ccatgtccat ttctatccgc tctcaacggg gtccccatgg
     1261 agttgctagg ggtcgattgc gaactcgcgc ccggcgcatg ctcgatgctc tcggacgatt
     1321 ggacagtgaa ctgagtgtgg ttcttgtcga cgacacgatc atgcagtcgc tcaatgccac
     1381 gtatcgcggc aaggatcaga cgacggatgt gttgtctttc gcgatgacgg aaggggattt
     1441 cggtgatgta aaccccgatg tattgggcga cgtcgtgatc tcggttccga cagcacgtcg
     1501 tcaggccacc caggcacgtc agccgcttct cgaccgagtt acttttttgc tagcgcacgg
     1561 tctgttgcac ttgatcggct acgatcatga aacagacgaa caacatcgtg agatgacgcg
     1621 acagacgaaa cgattgatgc gcaaagcgct ggatccgaaa cccattacca ataaaaagta
     1681 agtcctgcgc gcagcagtcc accacccgag ctgttggtgg tctggccggt atccggatcc
     1741 gtaaactcgg gattcgtgct ggcgaggcta tcggtacggg tgcggatgaa accgagcagg
     1801 tcgaggttga gcgcaacggg atgggacagg cgaaactcca atcccaatcc cgtatgcaga
     1861 ccaaaatagc tgtagtctac cgattcattc gatcgttcct ggatcttgac atgagcggcg
     1921 gaccaatcca gaccgccgag cacgtaaaat tgtgtggcat ctctgggatt ggcatacagc
     1981 aaggcggaca agctgaacgg caattcgcta cgtcgatatc cctggtagtc acgtcctccg
     2041 agcatatcga ggccgacatc gatggcgacg aaccgttcgg ggcgtaaacg aagggcgatt
     2101 ccggcccctc ccatgcccgt gctttcgttc gactcattgt ccatgagcac tcccgcaatg
     2161 tgcaaattga agccccacgg acggtatctt tcgcgttttt tgggaggcgg cggtggcggt
     2221 acgggaaagg gtgccgcggg gcgtcgcacc agcacgaccc tcggtggcgg cgggggagtc
     2281 tgttggtaaa cgacaatggg ctgagaggct tgggctccgg gaacgacgat ggtggtgggg
     2341 gcttgtttat tttcggccgc gggtggtggt ggcgtggggg caggcggggg caattcctgg
     2401 gggggagccg aatcaggcgc ttcttcacag aaccaagctc ctggagggca ctcctgcgtg
     2461 gtgctttgag cacgtgccag cgtgccgcag gtcagcagcc caaccaccat cgcataggaa
     2521 aactggctga tacgcatggg agtgactcct tgacgatgac ttcgagcgta gcaatgcgag
     2581 tgccagatgc cagcgcttcg tgagcgcgaa caaaacgcca cggatgcctt cgatagagtc
     2641 cgttgggttc cacggacgtg tgacgaatcg ttcacaaccc gcggcagaac atgccacagc
     2701 agtcgtctgt caggtatggt tcggccatgc cctcagtgat actcaaacct ggtcatgttc
     2761 agcccgtgtg gtcgggacac ccatgggttt attcccaagc cgtggagcgc gtgcaaggag
     2821 gggcgacggc aggcgatgag gtgacggtgc tcgatccgcg cggaaattac ctcggcagcg
     2881 ggtactattc ccctcgttcg gcgatcgtgg ttcggatagc cacccgtaat ccgaaagcgc
     2941 gcctcgacaa ctcgtggctt cgaaagcgct tgtctcaagc acacgaatta cgccacgaga
     3001 tggggctacc tacggtgcga acgaacgggt ttcgtctcgt ccatgcggag ggagacaatt
     3061 tgccggggtt ggtggtggat cgatacaacg agaccgtggc gatacagcta acgaccttgg
     3121 gaatgcatcg tcgccgggcg atggtgatgg acgcgattca gggagtgttt ggagtaaaaa
     3181 cgattgtgga tcggacgccg acttcatttg cgaagttgga ggggttcgaa ccggaaccag
     3241 ggatcgtatg cggcccgatg gatctcgacg cctatcgttt catggaacgg ggttttgtct
     3301 acgaggtgcc gatggctctc acgcagaaaa caggttatta tttcgatcag cggccgttgc
     3361 gcgcgcgggt ggaacaactc agtcgggggc gaagagtgtt ggacgcgttt tgtttcgtgg
     3421 gttcgtttgc tctcgcggcg gcgcgggggg gtgcaacgtc ggtgcttgcg gtggatgaaa
     3481 acgtcatcgc gctcgaaacg ggcgcgcgct gtgccaaggc caatggtcta tcggatcgga
     3541 ttgcgttttc gcgtgggtcg gttcgaacgc tgctttcttc ttcgcagcat accgccaagt
     3601 tcgatctggt cgttctggac ccacccagca tggctccttc gcagaagtcg ttgcagcgag
     3661 caagcaaggc ttttcgatgg ctcgtggcgc aaggatgccg tgttcttcgg cctggcggac
     3721 tgatggttct gagcgcttgt tccgcggcgg tggacgtgaa caccctgaca cgaatgcttg
     3781 ccatgggagc aagagacgcc ggaatgcagg cgatcgtgtt ggaacgctgg tttcagggaa
     3841 tggatcatcc cgtcccggct gcctttcccg aagggcttta tttgaaaagc ctcattgccc
     3901 gcatcaaccc gatctaaacc ccgagcatga ccaccggatc gttgcctcgt tgtatcggca
     3961 catcctcaca ccgaacacgg cgaatccatc gcggggtacc aacttgcgtg aacctgaact
     4021 aagcacaacc cacaaggccc gtgcatcgcc cttggctccg gtgtaaaccc ccattatctc
     4081 gcatgactcc catggttcat ctattccctg cgctacccag actccatcgg tcagagccga
     4141 ctcggtccac gcgccggggt cattaccgat cgaagaatcg ccgatgactc agcgagaaca
     4201 gcccctatct cgtcttcccg ctggccccat ggtggcgccc gtggattgcc ccggctgcgc
     4261 acgaaaaatc gatccgcttc gcgccggcca tgttgcaata tttgctaata acttccatta
     4321 cttctgtgat tattcctgcc gcgttcatta cctcacgcag catccggagt tggttcgtcc
     4381 caccccaaaa acaagtcttc cgccaggccc ccaggccctt gctcaatccc ttcccaccct
     4441 ggacgatgcg gagcctgtcg cgttcgatgc ggatactgaa ttgctcaacg tcgatgagca
     4501 agcggcgata ggggcaacgc cggcggtacc caagtcggac ccgggagcga tccttctcgt
     4561 aagcgcgacc gtgacggggg tattggcgat tgcgctcgcg ctcacgggaa cgggtctcct
     4621 tgcgttgacg attcgccttg ccatggcgtg tttgggtgtc gtgctgctcg tgattcggtc
     4681 tagcctatcg ccgcggactc catcggatcc ccatcctgcc gctctgctga gtcccgcggt
     4741 agcggcggcg atcattgccc tatgggctcg tatggtcggt cacgaaatag cggatgagtc
     4801 cgccgtgctt actgggttaa tcgtggtatc cacgggaacc tcccttcaac tgatcgagca
     4861 ggttcgtcgc ggcccgatgc atcggatatc gaccatccag caggtgttgg atgcaccggc
     4921 ccgtcgtgtg acgccctctg gatatgccat tgtggcatcg aaccaattgc ggcctggaga
     4981 ggagttggtg atcgacgcgg gggaggtggt gccggccgat gtagtgataa gcgcggggga
     5041 ggcgacggta cttccttggt tgggagcgca gtctgcgacg agcagggtag cggggcagag
     5101 tcttgtggcg ggttcgcggg tcgttacggg ccgtcttcgt gcgacggtga gttggacagg
     5161 aatggatcgt gcctggttat gtactacggc ggatcctcat cgtgcggcgc atgtgatggc
     5221 tccggtgacg cggcatgcgc gtcgactggt ggagcgttgg gcgcttgggg cagcggcggt
     5281 ttcggggttg gcggcatttt cgaacggttt gtcgactccc gcggtgttgt tggcggccat
     5341 cgcggcccat gcggcggtag cctcggcggc tacgggggca acgccatcgg tgcatgtgtt
     5401 gcgaggcgta ctaggggcgc tcgaacgtgg agtggcctat cgtcatgcgt tggcgtggga
     5461 gcgagccgct caatcgacgt tggcggtatt ttcggcgagg gggacgttgt tgctcggcga
     5521 gcctcacgta gcggaaatcg tcagtcttgg gcgtatgacg acggagcggt tgttatcggt
     5581 ggcggcggga gccgaggcgc tggcaagcga tgcgatagct ttggcgatca agcgagccgc
     5641 gctcgatcga caggttgaac tcgatgcggt gcggagtcct catgtggtac ctgggcttgg
     5701 ggttacggcg gtgacctcgg ctggtgagcc gctatgtgta ggaagccggg cgctgatgat
     5761 gcgcgaacat ctatcgatgg cgtcggtgga aaaccggcta gcggaattgg aggccatggg
     5821 gcggacggtc ttgcttgtgg ggattggagg aaaactgatt ggattcatag ccttacaaga
     5881 cggtttgcgt ccgggtgcgc gcgctgcggt tcagcatttg ctcgatgcag gggtggagcc
     5941 ggtgttgatg tctggcgatg cgcgtgagac ttgtgaggcg atcgcgagat ccttggatat
     6001 cgagcatgtt cgtcctgagg tacttccctc ggatcgggcc caagaactca agagcatgac
     6061 ggattcgggg gcgacggttg cgatggtggg ccgacttggg gaggacgagg cggcgttatc
     6121 cgcggcggat gtggcgattg tgctcgaggc ggcggggtcc gcggtcggag attggtccgt
     6181 cacgttggcg agtgatgatg tgcgagatgc ggcggatgga ttggtggcgg cgcggcgagc
     6241 ccgattgcat gcaagggcag ccatgtttct cggtttggcg ccgggagtgg cgagtgcgtt
     6301 ggccatcgcc tttgggcttc ttcctccggc tttcgccccg ttggcggtgc tcgtcagcgc
     6361 ggtgggaacc cacctacacc ttcgggcggt cgcgccatcg cagcatgacg acacgtccgg
     6421 ttgatgcaaa tacggcgatg gctcgtcgag cacgcccacc ttggcgaatc agccacgcgg
     6481 tgaaaggaac cgagttagag tcggccattg gcatggtgta ctgcacgcga gacatcctat
     6541 ttcggatttg ttttcgagcg gcagtactga ttttgtgttt gttgagtgct caaccatcgg
     6601 aggcgcaaga aacagaagac tcattgactc cgattcacgc tgcctcggat ctcggggatc
     6661 tgatcggcaa accgattcaa caagtgcagg tggtgcagac ggggcgttgg cgttcggaag
     6721 cgttggtgac ctcggtgaaa atcggcgagc ccatgacggg agcggcggcg cgacgagccg
     6781 cgcaggagtt gctaaatacc ggtggttttt ccgatgtgca ggtgtttgtg gagcggtcgg
     6841 gacagggggt tcggcttcgg ttggaggcgg taccgcgacg attgatagcg gcacgtcgtt
     6901 tggtgggttc cacgctggac gccacggagc tttggcaaac gatggggtta cgggacggtg
     6961 atgagcttac ggatgaaaag ctcgtccaaa tccaaaagca agtccaagac attcatcgtt
     7021 cccggggata tcctcgagcg cgggtgtcgg tacaaacgcg tgatacggat gcgcgagcaa
     7081 aggttgtgtt gatcgttcag gtcgatgcgg gtcctccgtt gatgatcgcc gatcgcatct
     7141 tcatcatgcc ggatgcgaca tggtccgttc tttcggatgt ggcgcaacgg tatcgtgtga
     7201 gcaaagcgga tcgattggat gaggaggctt tggcatccgc gaatgtggaa ttggaagaag
     7261 acttacgcgc gcgccgttat cccatggcgc gagttcggca ttcttcggtc gtgaaggacg
     7321 ggtttgggtt cgtctatgtg tatgtgcaag cgggtcctca tcttcgtttt cggttcgaag
     7381 gcaatgaacg gttcgatgcc gatcagctta cggatgtcct tgactacgac aagcaactgg
     7441 atcgaaccgc tgcggccctg accgatcgac tcgcatcgtt ttatcgtcgg tacggctttc
     7501 tcgatgtgga gatccacgcc gagtcacggg gcactgccac ggatttggtc aatgaattgg
     7561 tatttcacat ccgtgaaggt gagatcgttc aggtatatgg aaaagaattt ccgtgtcttc
     7621 gaggaggacc gcgaagcgtc gacgctttgt tggcggaggt ggatggcgta cttacgaacg
     7681 aactacctgg tggttcgttg atagggagtg tggatcccaa aatcgtggac gctaccttgg
     7741 gtccgaaggg agtgacgggg gcgcgccctt cgcctttgga gccagatccg acgcgaatct
     7801 acgacccagc cacgtacgac aaggttgttc agcacgtgcg ggacttgtac cgaagcgaag
     7861 gatttttgtc agcgatggtc ggtccggtgc gtgtgctcag aaggacatgc catcggcatt
     7921 cgccgccggg tcagtgtgta ccgttgccgt taccgtctcg tccgagcagt gcttgccaat
     7981 tcgatccgcg aggattgccg gtcgacgacg aggcgccgga tgcccgtttg caatgccgtc
     8041 cagatcgtgc gcgggccatc acgtgtgaac ctcgtgtgtg gttgcatatt ccggtcaagc
     8101 ttggacctcg agccatgttg tacgatgtgg cgttcgatgg gaatcgttcg atcccggaag
     8161 cggatctgtt ggaggcttcg gaactcgatg tgggtcaacc ggcatcgaac ctggaactcg
     8221 agaaagctcg acgtcgtgtg attcaggcgt accgggagat ggggtttgct tatgcggaag
     8281 tgcgagccac actcgatctt tcaccggatc acacccgcgc gcgagcacgc ttcatcgtca
     8341 atgaatcgca acaagtgatc gtggacaaga tcatcgtggt gggtgcggaa agaacgagtg
     8401 aaccgctgat tatcggacga gtatcgctga aaataggtca gccttatcgt gccagcgatg
     8461 tgcgcaagac cgaggagcgc attgccacgc ttggtacttt ttcgagcgtg gcggtatcgc
     8521 ttcaggatgc gtatgtgccc gcgcgacgca agactgtcgt gattcaggtg caggagcgac
     8581 tgccgcaata cttggacata cgacctgggt tttccacggg agaaggggta cgaattcaat
     8641 tcgagtacgg gcacttgaac attgcggggg aagccattcg tttcaccttg cgtcttcggc
     8701 tttcgtactt gcccgatgtc ttcatccttg atgaggatgt gcgaaataac ctcgacacgc
     8761 ttccgatgtc gcgtcgattg gagcggcaca atacggcatc cgtcctgttt ccggaaatag
     8821 gtctagggcc acttgtctcg ttgaacttcg atgggatcga cgtgcgttcg aacgcgcgag
     8881 actttggact gagcaagaat gcccttgcca caacgctgaa tattcgtccg tttcgcacct
     8941 tgtccttcgg tcttggaggt tcgttggagc gcaacgatgt ggccattttt agtgatcaga
     9001 gcgtgatcga gtacttgcaa cgcccgggag tcacggggga tcttcgacgt ttgttgttgg
     9061 ccccggatgg gttgacgttg gcggtggccc aattggccac ggtgtcttgg gatcgtcgcg
     9121 acaatccttt gggggccacg cgaggaacgc tgttgggggg ctctgtcgag catgtgcgcg
     9181 cgtttcctgg agaggataat ccgaatacga agctcagcga ttttttgcgc gtgagcggca
     9241 tcgtatcggc gtatttaccg ttgaacgaca atgggttgac gctggcggcg agcatgcgag
     9301 cgggccggat catccagatg atgtccagtt ccgacacgta tccggatcga ttgttttttt
     9361 tgggaggcgt ggatagcgtt cgcggtttct tgcgcgattc tgtcgttccc caggatgtgg
     9421 cggagcgaat tctccaaggc tcaccgcagg atccaaaccg gctgaccgtc aatcaggttg
     9481 ccatacgagg gggcgatgta tttttgaatc ctcggctcga gcttcgcatt ccgatgacgg
     9541 caaccgttca tacgacattg ttcatggact ctggcaatgt ctgggtagaa cctgaagcat
     9601 tcgaaccctg gaaactgcgc tacgcgtccg gttcgggcct tcgaattgcc acgccgatag
     9661 gtcctttggc ttttgactac gggatcaatc tggatcgtcg gtcatgggaa gactttggcg
     9721 cgtttcactt ttccatcggc ttgttctgac cggtttcgaa gctcgttttt cggggggaat
     9781 cgtaagcgtc tgaacagcat cgcgaggagt cctttccggc ggcgggcttc cgtgccatgg
     9841 tgtgagatat cgcttccggc gtcgaacgac cccgggagtc accatcgttt cgccattcat
     9901 cggtattcat tatctttttc gggagccgcc gtgcccacct tcgaaccttt cgagcatcgt
     9961 caagccttgg cccaacaagc aaagggtgtg ctgcgaaaac agatgcgggg cgtgcgggca
    10021 accataccga cggcagccat ggcgcagcga tcacgacagt tgcaaacacg aatcatgcaa
    10081 cttccgatat gggaccgtgt tcgaaccgtg ctgctgagcg aatcggccca ccaaaaaggt
    10141 gtatcgaccg catgggtagc gcaacaagcg cggtctcggg gtatcaccgt ggcgtaccct
    10201 cgggtggact acgacgccca acgtgtggag tatcgaattg ccacggaggc cgacttggaa
    10261 gatcgagggc tggaaacagc cgaggctccc aagaatgctt tcctcgttcc accgggcacg
    10321 atcgatctgg tcatcgtgcc cgttcttgcc gtcgacccag acggattctt cgtagacgac
    10381 gggtcggggg cctatgatcg cgtgctgcga gacctggcgc aggccatgac tcttgcggtt
    10441 gcgtttgaat tccagctcat cccggaggca ccgcgtacat cggaggatgt accggtcgcg
    10501 gtcatcgtga ccgaccgacg ggtcatcgag gctcgtaaca agcgggtgga aagcggatcg
    10561 gaaaacacgc accacaccaa ccgagccttg gaggaggatc gtcgtgccat tcaaccaaac
    10621 cttatggttg gtcctatcca tcgcgctgac ggcgctggtg gtgggactgg ccatcgtact
    10681 gggcaatgcc cgcaagaagg tttttgagct tcagcaacag attcgtcaca agggcaatga
    10741 agacgaacaa atagtacgtc aagccaaaga ggaggcagaa agaatcctgg cgcaagccag
    10801 actacaagga cagcgagaag ccgatacgat cacgttggaa gctcaaacgc agcttcgtgt
    10861 cgtggagggt aaaagccagg aggtggatgc gcgacgcaag gccgcggagg ttgaagctcg
    10921 cgaaatcctt ctcaaagcac gggatgcggc ggaaaaagaa gcccagcagg ccgctaccgc
    10981 gcagcaacaa gccctcgagg atctcgaaaa acgatcccaa aaacgagaac gggtcattcg
    11041 agaaaaagaa gacctcgtcg cttctcgcga actcgagctg acgcgacgtg aaaaaggact
    11101 gcaaacgcgc gaacagcagc ttcaatccga agaagcacgc atcgcggaaa tcattcacgc
    11161 gcaagaagac aagctgcaac agattgccgg aatgtcctcg gaagacgcca aaaatcgcct
    11221 catggagcga tataaggacg atgcccggcg tggggcggcc aacgatgcca agcgcatcga
    11281 ggatgaagcg cgcgaggagg cggaaaagaa agccaagcgc atcatcggcc tcgccatcca
    11341 acgattcgct ggtgagtatg tccaagaccg ctccgtcaca tccgttcact tgcccaacga
    11401 cgaactcaag gggcgtatca ttggacgaga gggacgcaac attcgggcat tgcaagaagc
    11461 caccggcgtc gacttcatcg tcgacgacac accggaaacg atcgtggtat cgagtttcga
    11521 tccgattcgg cgggaagtgg cgagaatcgc tctcgaaaaa ctcattgtgg acggtcgtat
    11581 ccatccgaca cgcatcgaag aggtggtgac caaatcgcaa gaagaggtgg agcgagcgat
    11641 gcgagaggct gccgagcagg ccatgctcga gctttcgatc acccgcgttc atcctgaaat
    11701 cgtcaaactt ctcggtcggt tacgatatcg gtacagctac gcacaaaacg tgctcaggca
    11761 tagcgtcgaa tgcggctttc tctgtggact gatggcttcc gaactgggac tcaacgtccg
    11821 tcaagcaaga cgcgccggac tatttcatga catcggtaag gccgtcagcc atgaacaaga
    11881 aggcggccac gcggtgattg gcggccagat cgctcgaaaa tacggagaag atgaagtggt
    11941 ggccaatgcc attgcctgcc accacgatga cgaaccgtgc aagcatctgt tggggcatct
    12001 ggtggctgcg gcggatacgc tttctggcgc acgccccggc gcacgacgag agatgctgga
    12061 gtcttatgtt cagcgactcg aagacctgga acggatttcc acctcgttta ccggggtcga
    12121 gcgagcttat gcgatccagg cggggcggga ggtacgtgtg ttggtcgaac catccgaggt
    12181 gtcggatgcc gaaagcgctc tgctggcacg ggagattgcc cgaaaagtgg aagagtccct
    12241 cacgtatcca gggcagatcc gggtgacggt cgtccgagaa acgcgagcca tcgactacgc
    12301 caaatagaca cctctgcctt catccccaaa ggcgatactt cttctcatat ccagcatcca
    12361 ggttgcgaat cgaaacctcc acgattccgt ctgcatcgac cgtgtatcgt tcttccacaa
    12421 gaggaccagg actggccaaa cgtcgaacgg gtatcgtgcg aagatcctct ttcccttcac
    12481 aaagcatcgg atcgaatgga aataacgcct ccgcaaaggg tgtgatatcg cccgaaggaa
    12541 cccccgacga atcgatatca tcgcattcga catatcgaaa gtgcccgata ttatgggcag
    12601 cacgataggt tcgactcgat gtcacacaac gttcttgcga caggggcatg catgtatctt
    12661 tggtgaatac cggatcgaag gagacgaccg caccgtcccg aagctcacga aatactccaa
    12721 atacccgcga aaaacggtct gccaatcgat atcccgcttc cttgtccccc gcgatcgcaa
    12781 gcccaatggc tgtcgccgca gagggataag gagaacgatg aactcgtcga ccgtacttat
    12841 gcttgagcgt ccgcccaacc gcgggcaaag cactcgctcc tcccaccacg taaataccag
    12901 caataccaag caaatttctc gtatcgccgc caccctctcc gcgttcgacc acatgcatca
    12961 tcgcctcgat actctgttcg atcagcggcg tgcaggcctc gtaaaacgtg gatacgggta
    13021 acgcgacttc ccgcacgggc gcatgctctc cccacgcgga ttccacatcc accaatacgc
    13081 gacgagaccc ttggaccaaa ctttctttgg cttcgcgaca cacgctaacc aaccgctcac
    13141 gacgtggatc gctttccgac agctccatca tacctttctc gccgagcacg agatcggcca
    13201 ataccgtatc gaaatcgtcg cccccaagac gcgggattcc agacgtatac acgatatcgt
    13261 ggttctgacc gttcatgcgt accaaagaag catcgaacgt cccaccgccc aggtcgtaca
    13321 ctacgacatg ctcacgtttc gaagtgatcg tggaacgata acgatgagcg tattcaaagc
    13381 cggcggcaga aggctcattg agcaaagcaa gaacttgaaa gccagacttc tgaaaggcgt
    13441 cgatcgtgag aaaccgttgg gtgccatgag cattcgcggg ggtggcaatc atcacgggaa
    13501 tgctttccaa cggatgcaat gcagcctgca tgctcgattg cgaccgcaga tcgttgtgaa
    13561 gcgagcgaag aaaagttacc agcagatcga tcaagggcat acgaaggctt cccaccttga
    13621 caacatggtc cggtcgaacc gtgggttccg tcagcattcg cttgaacgat cgcagcacga
    13681 cccacgaggg gtcagaggcg cacgcatgcg ctgcccaacc acagcgcaac tcaccatttc
    13741 gctcagcgac gagcgatgga taccaatccg cgaagtcacc atcctcgctg gcgaaagaga
    13801 cgatgggata attgcctcga tcgcatgcag cgacaaccgt acgcgtggtt ccgaaatcga
    13861 ttcctagatg cacggcagct tcctatggct cttactaccc atgcgtattc tacgaaacaa
    13921 tcggtacgag cgggggaaca gtccacgcag ggcgagcttc atgatgaact cgatccccac
    13981 ggacccacca tgatccttcg ggacggatcc aatcgtgatg ttgcagccac gtaagcagag
    14041 gctgggattc cgacggtcct atcagcgctt cgtataatgc gacgagctgg cgggttgaaa
    14101 accgttctgg aagcaacacg agggggatgg acgactgggc agtttgcagg cgaacgcgtt
    14161 gccagacctt ctgcaagacg acatcatggt cgaaggcaag gcgaaggggc ttgtcttgct
    14221 tgtccaccgg aatgccttga cgaagagaaa cgaattcagc cgaagccgcg tcatcccctc
    14281 cttggggttc gggcaaatcg ccatgccgca cccacgcgag aaatacggcg ctcaccacgc
    14341 gacttcgagg atctcgccat gggtttccga atgcacccaa ttgttcgata ggtgtggatc
    14401 cgagatctcc aattcccgtc tcttcctgaa gctcacgacg tgcggcggtt tccaggtcct
    14461 catccatgtc gacgaatccc ccaggcaacg cccactgccc ttgaaatggc ttgttttttc
    14521 gctgaattag caaaagccgc agatcgacct gctcttccca ccagattgcc gtgagcacgg
    14581 caagatcggc ggccagattc gggcccaacc cataacggct ttgtatctcc gcgatttcct
    14641 cacgcgtcat tccgtgatca tctctgcctg caccgaccat ccccgcacct cgagtgttgg
    14701 tgttcagata atcttgtgcg acgagccctg cgcaaacgca agggggatgt tttcgtggcg
    14761 cactcgttac cctctgggtg ggcaacgttg atctacgaat ggcgcccaag tgagtgatca
    14821 cggtatttga aaagtctgac ggcgcaaacc aaagtcgcat gcgttcgagt ttcatcccaa
    14881 cctcatgcga tcgccactag ccgagcgctc gacgtcga
//
LOCUS       DAOSOR010000062        14769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4090_length_14769_cov_4.397988, whole genome shotgun sequence.
ACCESSION   DAOSOR010000062 DAOSOR010000000
VERSION     DAOSOR010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 14769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14769
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4090_length_14769_cov_4.397988"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PK710_05175"
     CDS             <1..195
                     /locus_tag="PK710_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09139.1"
                     /translation="VSERNPSSSGKKGRQDANFASARVNLRPSDGAGIDEPKDRFGLS
                     VSGYLLYAVVCASHWTGALR"
     gene            complement(342..746)
                     /locus_tag="PK710_05180"
     CDS             complement(342..746)
                     /locus_tag="PK710_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09140.1"
                     /translation="MNRVLISVLAGFLCCACSKSENVEEATGDQSGASPVVGEEAPKA
                     DTPAAQPADSSKAEKPSVANREGASGNLNANKDEVTAGDVKVKKDGVTTGDVKVDKDG
                     VTTGGVKVDKDGVTTGGVKVGKDGKISVPGLR"
     gene            complement(828..1868)
                     /locus_tag="PK710_05185"
     CDS             complement(828..1868)
                     /locus_tag="PK710_05185"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOT09141.1"
                     /translation="MTGTLRRQLQSRFGDAGHGYMPIANPWPGYFHNDVVRRASREWK
                     VSRVVGPFAADGLYGLGGVSFQGFNRAAWARYATTQEGEFGRSVSRFGVQYLEHPGGG
                     TIELEIDKEKTITIETSGENAVLREHVVQMADGAHQIELRVAKGPVRAFGTWMERDVP
                     GVVLDSIGIQGGRLRFLDQSNDEHYAQALQMRNPQLIVFEFGLNESADGFAYPMDRYR
                     ETSLSVMRKMRKATPHAACLVVAPNDVARKEGTQIVSARIMPELVKAQREVAAEVGCA
                     FFDTHEAMGGVGSMPTWVRRGLGQPDLTHPTTMGADVIGTWIYRALMDAYEPYRAKHG
                     GTSSPPAASREP"
     gene            complement(1825..2337)
                     /locus_tag="PK710_05190"
     CDS             complement(1825..2337)
                     /locus_tag="PK710_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09142.1"
                     /translation="MAPWIAPVAREALSSKPVLASLTVLAIIGVAYLVPGAERFRLFN
                     PKMGEASPPAAVSDQASAPVGEANLAEETKDRPELALPGSPAAVQETPGLPPLPHAIR
                     EKAPPVPIVDPSGKALDPFFHALVAAERKEKGAIVRIVHYGDSLLAVEKPSSRLCDRY
                     ASTTIAEPVW"
     gene            complement(2524..3906)
                     /locus_tag="PK710_05195"
     CDS             complement(2524..3906)
                     /locus_tag="PK710_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOT09143.1"
                     /translation="MRNTYFFIPALFAALAACGGESDSPQDTDNPNNNTDKPQVARSE
                     LERAPASSVSSDTVDAIVQADNAFAFDLFAKARASAKSSNTVMSPLSVSIALGMTWAG
                     ARNQTAEEMAKTLHFDKPGLDVHAGHNALTQTLESRAADALANAKEKAKLMGSSAPSE
                     DNFRLHIVNSVWADGSYTWERPFLDTLAVNYGAGVFLADFIHEYDAERERINTWVSNE
                     TQNKINNLLPDGSLDENTRMVLVNAIHLRMPWELPFEKDFTKPGDFTTADGSMVTAEF
                     MSQDAPFGYYEDAAAQVVSLPLVGRQLSLVVALPKDSLESYEAGLTAASWKAVWEARE
                     TKPVDLALPKFSFTTDAIKLKEVFQQLGMVQAFEAPMADFYGMCSQPPNGERLYIDEI
                     MHKAMMAVDEKGVEAAAATAVIMAGETSAPPESIEMKVNKPFVVAIMDEPTGAVLFIG
                     HIQNPLSEGM"
     gene            4114..4914
                     /locus_tag="PK710_05200"
     CDS             4114..4914
                     /locus_tag="PK710_05200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HOT09144.1"
                     /translation="MQVPRVFFLFRSLLVVAAVLMGWATPAFAFGSFNLKQTAVEEAD
                     GRWKFNVDVDYGSKPHLGHVPFDFVFVQKVYYEYSITDTDKAPVQRRKPMHNQEPQRE
                     QMDIAFSDARGETWQRTKFSFAVRRDRGFSAGEYTLTVKRSSDGAVLGRPMNITLNGQ
                     NELVDRRSIVFTGDVKKKPETKPAEPDKESEEPTREASQQATDTVPEDVEPEDTESAP
                     GPAPVEKKAKPAGCGCSVPGRDRTQGALAMLGIMLLGWTVFWRRRGCC"
     gene            complement(4937..5716)
                     /locus_tag="PK710_05205"
     CDS             complement(4937..5716)
                     /locus_tag="PK710_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09145.1"
                     /translation="MEFAIDHVPVEARARYTELGRQFGCVDTLAQAKLTLLAFERYGD
                     QVARHGFIQLDADRLREVRDLLHQMTLQATDEDGRCKKTSQEYVQAIRGGKDARQRAR
                     SILYIIAQRLALCGDFIAAEAARAVVATLQQTESSRGDPFRLAAQLDQLRATLTIPRV
                     DKEAERRGGPDAAQALVHAAEKLRAIANRHSVETHVRQPDNDFDLLCGMIVELVRDAR
                     RAARSAAREQNAECIAAAFALTKLYGPAASGVDSERSPTLR"
     gene            complement(5759..7297)
                     /locus_tag="PK710_05210"
     CDS             complement(5759..7297)
                     /locus_tag="PK710_05210"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT09146.1"
                     /translation="MALPMWTLTIMDDEGKQTQLPLSRGEYAIGRAEENTVRLTDRNI
                     SRKHLMLRRNGDDGWLVEDLDSYNGCFVNGMRVSGTHPLYHGDLLQLGDYRLELRDES
                     ALVDASGEKTQEILNAPVSTLLDRPDRLVVVDGVAAGTEYPLEGERIIIGRAEEADIS
                     INHSSVSRTHAEIVNLGAGIYELFDRGSSNGVRVNGTRFERRVIEDDDEIELGDVRLR
                     FVARGRIFRPGGPPRSFNYSTATSSAPGAVRRKGSMGVMIAVGAALGVAVVIGFAALR
                     SWDSSPSGASTERVVEDQSVLEEAKRLFESGNIDGAHAKLSQLPDGSPLRDSNVVRDI
                     EGKWADDVFARAATTSDLVRRRAWLEQVSSAPLVDAERRKRAADMIAQMDTGGTDIGS
                     LPVAEAKVKDAGRPTVLAANPFDEPKTPGTAKTGKPSSTSTDVPATPPTTTSTTGTAD
                     NAREAILTDNDGESRARRMLEPKVWSGRATVDEIKMLRAICRHQRDSACVAKCTALLK
                     QKQE"
     gene            7679..9196
                     /locus_tag="PK710_05215"
     CDS             7679..9196
                     /locus_tag="PK710_05215"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOT09147.1"
                     /translation="MNQIVVFTSSSILSTARGAEAALEQAAHQFSPRLARGIRIVVET
                     NASHLLARLRSHDVDVLVIDARQETASQTPSSSQVLMSQLFTESSAGDLIGREQTWLI
                     VPPNRWGTELSFQAGVFRLAGILALEQSDQAWRTIWTNLEKTLRRREAGRVALCLAGG
                     GFEGLFFELGVLRALQHFLPEYALQDADIVCGISAGAVIGAFLANGLSASDIMSGMLR
                     GQGTLDRIERSSVFDLNYAELSSRFGRAARSLARRRTTLLQSFFHLAPTGIFAGKRLR
                     SYLQLQLSKPGLTDDFRQLPHGLYIGATDQDTAEHIVFGSPGWDHVPIHQAVRASTAL
                     TPIFAPELIDNRYFVDGAFTRTTNVRIAVENGATLVILVDPLVPTHNDQPGHVFEKGG
                     IMVGMQGLKSLIHGRFDRAIHMLRAMYPHVAFHLFQPDGATMRVMAGAPAKIFHRPEV
                     EEIAFRETLRSIRQYRFEALQRDFTRHAIAFTDPQTDLGTIKRDLLDQAAEVQVA"
     gene            9193..9951
                     /locus_tag="PK710_05220"
     CDS             9193..9951
                     /locus_tag="PK710_05220"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOT09148.1"
                     /translation="MRFRRIISLVPSDTFNLARLGLLHQLVGRTRYCVVPDGVQQIPV
                     IGGTKDVSVRDVIALAPDIVLANQEENSKSDVLALRAAGLNVRLTFPKTVEDGFQDFE
                     ALAACLGLDRPAIARPAPCPMSTRKCFVPIWRLPWMTINGQTYIGDVLRWLGLVNVFE
                     HLPSEDAQGKDSRYARLTMDQMLQAQPDVVLLGDDPYPFGSEHVHYFRSLDIPAARTG
                     AIFRVSGRDLCWPGAWAEHGLLRLRKVMSVRDEP"
     gene            complement(10031..10222)
                     /locus_tag="PK710_05225"
     CDS             complement(10031..10222)
                     /locus_tag="PK710_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09149.1"
                     /translation="MIGLELLKKERELLKNKLLEIEAESKALDAKVRSLRQKEIQTKR
                     EIEALCVLIELQEEAKTES"
     gene            complement(10238..11398)
                     /gene="glgA"
                     /locus_tag="PK710_05230"
     CDS             complement(10238..11398)
                     /gene="glgA"
                     /locus_tag="PK710_05230"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HOT09150.1"
                     /translation="MKVTIISKEYPPHVYGGAGVHVKFLTRELAKRMQVEVRCFGEQR
                     MSDGNLTVKGYQAWDRMWEGTDRRFNSTLGTFSTDLSLVRDHVDSDVVHTHTWYAAMA
                     GYMAKVLYDIPLVCTVHSLEPLRPWKEEQLGKSYHLSTWVEKVALENADRIISVSQGS
                     KKDVLDHFNVDPERVRVIHNGIDLDLYRPVHGDSTRKAFDIDGPYILFVGRTSRQKGM
                     TYLIDAMASVDPGVRLVCCTSAPDTKEVEEEIAEKVAKEPRALWINSLLREEQYVELY
                     ANASVFACPSVYEPFGIINLEAMACETPVVASRVGGILETVVDGETGYLVEPANPRAI
                     ADAINTLLRNPEMAKKFGRNGRRRVEDHFSWTHIAEKTEALYAEMVEERRRG"
     gene            complement(11414..12700)
                     /locus_tag="PK710_05235"
     CDS             complement(11414..12700)
                     /locus_tag="PK710_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOT09151.1"
                     /translation="MRKTIAIVFAGGRAEEMSVLTFWRPKSAVLFGGAYRMIDFALTN
                     LANTAGIDNVGILSQYRPSSLIDHVGIGLPWDFVGNERGVRFLPPYIGLDHSQWYRGT
                     ADALYQNIDFLELHRPDDVLIVSGDHAYSMDYTPLLRFHMERDADVTLAFTPMDVEPS
                     RFGIAELNAEGRIIGYMEKPKHPRTNLASMTVYVFRREVLVEELRRHAHATPEEQKTY
                     QIYDEILPHMMAHRRAFGWVHYGQWEYARTLDAYHAAHMDMLGPSPRIDLSSWNVRTN
                     TMMRRAAPPPPTRCFSGAEVVDSLIGGGCNIQGRVERSVLSSDVRIGRGAIVRNSVLW
                     EKVVVEDGAVVDGVICDKRCVIGRGARVGADMGNLVANEELPGSLQGGATVLGMDVHV
                     PAGGLLGKNCIIYPKTSSDLLCDPVASGRTVRGAAH"
     gene            12911..>14769
                     /locus_tag="PK710_05240"
     CDS             12911..>14769
                     /locus_tag="PK710_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 domain-containing protein"
                     /protein_id="HOT09152.1"
                     /translation="MSSLSPRLIHLASLWQRACGPWRGRLFAASLLLIIVAAAHLARH
                     GTISSRLLAAAIVVVTCVIAVVLRKMAQRQARNLIRLAKKLGSLEHPSLGERAARAVR
                     LLTTTHGSDDALSAGLAHLHLERLLAQIRPETLWKAAKKRADVAKIVALSLLALALGA
                     FAYSPLRVAEGVDVLLAYRGRAPIDIDWVESAVVDIQPPAYLGESIQHAMAFDETYQP
                     VGTKLSVHARPRFDGRTLVLTDGKRETPFVDDAKGGIVATWTVEHDATLEIAARFGDV
                     LIAQSTSLRVVALEDEAPKVIVDDTPRTVRLLDIQEVPIRYEITDDHGLTHVELVLRT
                     AGKESRRSLAKHDGTLTFDRGATVLHRQDRVLVQAHTPVEVTIEARDNNAVGGPSWGK
                     SQPIVVLPPAVGEEEALRFEAVQSLRNDLLDLLASRLRREAPSQHEDRAALNTKETKA
                     HASFEQAANQVSQQSYGGIVVSRRMRAFIQGQLERLRKATDGACPTLSEQACRSGLRR
                     LQDVTGDVALALDVATRTLSTRDSIAIARKLADVADEAAEGARQIRQSESQRGRVRLD
                     ASLEVLRGGSGSMLRLGGLGGDLGEIVAMGVRNIQRCMHRDDTQCAESAALHLA"
BASE COUNT         3075 a         4433 c         4009 g         3252 t
ORIGIN      
        1 gtgtcggagc gaaacccctc ctcttccggc aaaaagggtc ggcaggacgc gaacttcgcc
       61 tctgcgcgtg tcaacctccg gccgtccgat ggagcaggca tcgatgaacc gaaggacagg
      121 ttcgggctct ctgtttctgg gtacttgctg tacgccgtgg tatgtgcctc gcattggact
      181 ggcgcactgc ggtgatcaac atgggatgat tgtgagcgct ttgcgtctgc cgtgaccgtg
      241 acggaaaggc tcggggaaaa atgaggggat ggctcagggg ggaaagggca acggtatgct
      301 gtttttcaat tcggtctgtg gtgaggcctt ggctcgcaga cctaacgcaa gccaggaacg
      361 gaaatcttcc cgtctttgcc caccttcacc ccaccggttg tcaccccatc tttatcgacc
      421 ttcaccccac cggttgtcac tccatccttg tctaccttca catctccggt cgtcacccca
      481 tccttcttga ccttcacatc cccggccgtc acctcatcct tgttcgcgtt caagttgccg
      541 ctggcgcctt ctcggtttgc gacacttggc ttctccgcct tgctggaatc ggcgggctgt
      601 gccgcgggcg tgtcggcttt gggagcttct tcccctacca ccgggctggc gccggattga
      661 tcacccgtcg cctcttcgac attttcgctc ttggagcaag cacaacatag aaatccagcc
      721 agcacgctga tcaagactcg attcatggca ataacctccg aaggaagggt aattatagat
      781 gcccgtaacg aaaaagctgg aaaaatatgg cgaagggagg aaaaacctca cggttctcgg
      841 gaagccgccg gcggcgaaga tgtcccccca tgcttggcgc gatacggttc gtacgcatcc
      901 atcagcgctc ggtaaatcca cgtcccgata acatccgccc ccatcgtggt gggatgcgtc
      961 aaatccggtt gaccgagacc gcgacggacc catgtcggca tcgaacccac accgcccatg
     1021 gcttcgtgcg tgtcgaaaaa ggcacaacct acctcggccg ctacctctcg ctgcgccttg
     1081 accaattcag gcatgatccg cgcgctgacg atttgcgtcc cttctttgcg cgccacatca
     1141 ttgggagcca cgaccagaca ggcagcatgg ggcgttgcct ttcgcatctt gcgcatcacg
     1201 gacagcgatg tttcccgata tcgatccatc ggatacgcaa acccgtcggc gctttcgttg
     1261 agtccaaact cgaagacgat gagctgagga tttcgcatct gcagcgcttg cgcatagtgc
     1321 tcatcgttcg attgatccaa aaaccgcaac cggcctcctt gaattccaat ggaatccagc
     1381 acgacccccg gaacgtcgcg ttccatccac gttccaaaag cccgtacggg tcctttggcg
     1441 actcgcaatt ctatctgatg agccccatcg gccatctgca ccacatgctc acgcaatacc
     1501 gcgttttctc ccgacgtctc gatcgtgatc gttttttcct tgtcgatctc gagttctatc
     1561 gttcccccgc cgggatgctc caggtattgc acgccaaaac gtgataccga acgcccgaat
     1621 tccccctctt gagtcgtggc atatcgcgcc caggccgctc ggttgaatcc ctgaaacgat
     1681 acacccccaa gaccgtacaa cccatcggcc gcgaaaggtc ccacgactcg gcttaccttc
     1741 cactcacgag aagcgcgtcg aacgacatca ttatgaaaat accccggcca tggattggcg
     1801 atcggcatat atccatgacc cgcatcacca aaccggctct gcaattgtcg tcgaagcgta
     1861 ccggtcacaa agtcgactgc ttggcttctc gaccgcgagc agcgaatcgc cgtagtgaac
     1921 gatgcgaacg atggccccct tttctttgcg ttcggcggct acgagcgcgt gaaaaaaagg
     1981 atcgagtgcc ttgcccgacg gatcgacgat gggcacgggc ggagcctttt ctcggatggc
     2041 gtggggtagg ggaggcagcc caggggtttc ctgcacagcc gccggactgc caggaagcgc
     2101 caactcggga cggtctttcg tctcttccgc aagattcgct tcccctacag gagccgacgc
     2161 ttgatccgat accgcggcgg gcgggctcgc ttcccccatt ttgggattga acaacctgaa
     2221 acgctctgcg ccgggcacaa ggtatgccac accgattatt gcgagcaccg tcagcgatgc
     2281 caacaccggt ttcgatgata acgcttcacg agcaacggga gctatccatg gagccacgtt
     2341 ccgcacctgc cagccgtcga gttacaaccg cttgcctatt cgagttccaa gcgtttgcct
     2401 agcatacgat gaacagaatc gttcgcagcg gtgtcacaag cggtcgtgag gggaaaaact
     2461 gtttacatgg ctcccgcgag cgtacgacaa gcaagggttg ccgaaaaaaa cgaatcctgc
     2521 ggcctacatg ccttcagaca ggggattttg aatgtgcccg atgaacaata cggcacccgt
     2581 gggttcgtcc atgatcgcca cgacgaacgg tttgttgact ttcatttcga tactttctgg
     2641 cggagccgac gtttctcccg ccatgataac cgccgtggct gcggccgctt ccacgccctt
     2701 ttcgtcgacc gccatcatcg ccttatgcat gatctcgtcg atgtagagac gctcgccatt
     2761 cggcggctgg ctgcacatac cgtaaaaatc ggccatgggc gcctcgaaag cttgcaccat
     2821 gcccaattgc tggaagactt ccttcagctt gatcgcgtcg gtggtgaacg agaacttggg
     2881 caaggcaaga tcgacgggct tcgtttcgcg cgcttcccat accgctttcc acgaagctgc
     2941 cgtcaaaccc gcctcataag actccaatga atccttgggc aacgccacca ccaacgatag
     3001 ctggcgtcca accaacggta gcgacaccac ctgcgccgcg gcatcctcgt aataaccgaa
     3061 aggcgcatcc tgagacatga actccgccgt gaccatcgaa ccatcggcgg tcgtgaaatc
     3121 gccaggtttg gtaaagtctt tttcaaacgg caactcccac ggcatcctca gatgaatggc
     3181 gttgacgagc accatgcggg tattctcgtc gagggagccg tcagggagta agttgttgat
     3241 tttattctgt gtttcgttac tgacccaggt gttgatccgc tctcgttccg catcgtattc
     3301 atggatgaag tcggccagaa atacgccggc accgtagttg accgcgagcg tatccaaaaa
     3361 gggcctttcc cacgtatagc tgccgtctgc ccataccgag ttgacgatat gaagtcggaa
     3421 gttgtcttcg gaaggcgccg aagaacccat gagtttggct ttttctttgg cgttggcgag
     3481 cgcatcggca gcccgggatt cgagggtttg cgtcaatgca ttatgaccgg catgaacgtc
     3541 caatcccggc ttgtcgaagt gaagcgtttt tgccatttcc tcggcggttt ggttgcgagc
     3601 acccgcccac gtcatcccta aagcgatcga tacactcaac ggggacatga ccgtgttgct
     3661 ggacttggcc gatgcgcgag ctttggcaaa tagatcgaaa gcaaacgcat tgtccgcctg
     3721 tacgatggca tcgacggtat cgctggaaac cgaggaggcg ggggcgcgtt cgagttccga
     3781 acgggcaact tgtggcttgt cggtattgtt attcgggttg tctgtgtctt gtggtgagtc
     3841 tgattcgcct ccacaagcag ccaaggcggc gaacaacgcg gggatgaaaa agtaggtgtt
     3901 tcgcatggat ggagccacaa gcaaggctcg tgccgcttcg cgttttcctc gtgggactgt
     3961 tgttttttcc aggcgggaag gcaggtggcc tgttcacctc gggcagcgat gcctcatttg
     4021 gcatgggctg tcacacagtg gcacagaaca tcgggaggaa agaatcggcg atgcagtgga
     4081 aaagccttga gtcgttaggg tatcctgttc gccatgcaag tacccagggt gttttttctg
     4141 tttcgatcac ttctcgtggt agcggccgtg ctgatgggat gggctacccc cgcttttgct
     4201 tttggttcgt tcaacctgaa acaaaccgcg gttgaagaag cggatgggcg atggaaattc
     4261 aatgtcgatg tcgattatgg cagcaagccg cacctcggcc acgtcccgtt cgatttcgtc
     4321 ttcgtgcaga aggtgtatta cgaatactcg atcacggaca ccgacaaggc tccggtgcaa
     4381 cgtcgcaaac ccatgcacaa tcaggagccg cagcgtgagc agatggatat cgctttttcc
     4441 gatgcgcggg gcgaaacttg gcagcggacg aagttttcgt ttgccgtgcg acgggatcgt
     4501 ggtttttccg ctggtgaata cacgttgacc gtcaaacgat cgtccgatgg cgccgttctt
     4561 ggccgtccga tgaacatcac gctcaacgga cagaatgaat tggtcgatcg acgttcgatc
     4621 gtatttaccg gtgatgtgaa gaaaaagccg gaaaccaagc ctgcggaacc cgacaaggaa
     4681 tcggaagaac cgacgcggga ggcgtcgcaa caagcgacgg atacggtgcc ggaggatgtg
     4741 gagccggaag ataccgaatc ggcgccgggc cctgcaccgg tggaaaagaa agccaagcca
     4801 gcgggatgcg gttgttccgt gccaggccgc gatcggacgc aaggtgcgct tgcgatgttg
     4861 ggaatcatgc tattgggctg gacggttttt tggcgacgtc gcgggtgctg ctagatcgat
     4921 gggatactgc gctcgatcaa cgaagggtcg gagatcgttc ggaatcgacg ccgcttgcag
     4981 cagggccgta gagcttggtc aaggcgaaag ccgctgcgat acattcggcg ttttgctcgc
     5041 gagcggcaga gcgcgcggcc cggcgcgcat cacgaacgag ttccacgatc atgccacaca
     5101 aaaggtcgaa atcattgtcg ggctgcctga catgcgtctc gactgaatgc ctgttcgcga
     5161 tggcccgtag tttttcggcg gcatgaacaa gagcttgggc cgcatctggc ccgccacgac
     5221 gttctgcttc tttatcgaca cgagggatgg tgagcgttgc ccgaagttgg tcgagttgcg
     5281 ctgccaatcg aaatggatca ccacgggagg attcggtttg ctgaagggtt gccaccacgg
     5341 ccctggcggc ttccgccgcg atgaagtccc cacacaatgc aagacgttgt gcgatgatat
     5401 aaaggatgga acgcgcccgc tgtcgcgcgt ctttcccacc acgaatggcc tgcacatatt
     5461 cctgggaggt ttttttgcag cgaccgtcct cgtcggtggc ttgcaacgtc atttgatgca
     5521 gcagatcccg aacttctcgt agacggtccg cgtcgagttg gatgaaacca tggcgggcta
     5581 cctgatctcc atagcgctcg aaagcaagga gcgtcagttt ggcttgcgcc aaggtatcga
     5641 cgcacccaaa ctgccggcca agctccgtgt agcgtgctcg cgcttccacg ggcacatgat
     5701 cgatcgcgaa ttccatcgtg aatcctgatt ccccgtaggg tcggcagacc accggctttc
     5761 actcctgctt ttgcttgagc aatgccgtac acttggcaac gcaagccgaa tctctttggt
     5821 gacggcagat cgctcgcagc attttgatct cgtcgaccgt ggcccgtccc gaccaaactt
     5881 tcggctccag catccgacga gcgcgactct cgccatcgtt gtccgtcaaa atggcttcac
     5941 gcgcgttgtc cgccgtcccg gttgtgctcg tcgtcgtcgg aggtgttgcc ggcacgtccg
     6001 tagaagtaga cgatggtttc cccgttttgg ctgtcccagg ggttttgggc tcatcaaacg
     6061 gattggcagc aagcaccgtc ggtcgaccag catccttgac tttggcttct gctaccggaa
     6121 gtgagccgat atccgttcct cccgtatcca tctgagcgat catatccgcc gcgcgtttgc
     6181 gacgttccgc atccacgagc ggagccgatg acacttgctc caaccaggcg cgacgcctga
     6241 ccaagtccga tgtcgtagcc gctcgagcga agacatcatc ggcccacttt ccttcgatat
     6301 cacgtaccac attgctgtcg cgaagcggtg agccatcggg aagttgcgag agcttcgcat
     6361 gggcgccgtc aatattgcct gactcgaaca gtcgtttggc ttcttcgagc accgattgat
     6421 cctcgaccac tcgctcggta gatgcgcccg aaggcgagga gtcccacgag cgaagtgcgg
     6481 caaatcctat caccaccgca actcccaacg cggccccgac ggcaatcatc acgcccatgc
     6541 ttccttttcg gcgcaccgcg cctggagcgg aggaagtggc tgtcgagtag ttgaaacttc
     6601 gcggcggacc cccaggacgg aaaatacgtc cccgggcgac aaatcgaagc cgaacgtcgc
     6661 caagttcgat ctcgtcgtcg tcttcaatga ctcgacgctc gaacctggtt ccattgacac
     6721 ggacgccatt ggaactgccg cggtcgaata gttcgtatat tcccgcgcca aggttgacga
     6781 tttcggcgtg ggtgcgactc acggagctat gattgatgct gatatcggct tcttccgcac
     6841 ggccgatgat gattctttcg ccttcgaggg gatactccgt tccggctgca acaccatcga
     6901 caacgaccaa acggtccggt cgatcgagca acgtggaaac cggagcgttc aggatttcct
     6961 gagtcttttc tccagacgca tcgaccaacg cgctctcgtc acgcaactcc agacgataat
     7021 ccccgagctg cagcaggtcc ccatgataaa gcggatgagt ccccgaaacg cgcatcccgt
     7081 tgacgaagca tccgttgtag ctgtccagat cctcgacgag ccacccatcg tcaccattgc
     7141 gacgaagcat cagatgcttg cgcgagatgt ttcgatccgt caggcgaacg gtattttctt
     7201 cggctcgacc gatggcatac tcgccgcgag acaaaggaag ctgtgtctgc ttgccttcgt
     7261 cgtccatgat ggtgagtgtc cacatcggta atgccacggt ccttacagtg ggtgtcgagg
     7321 tcacctagat gatagtgtag ccagcctgct catggaacgg caacctttgc tagcgattct
     7381 ttgttctttc tttccgaatt tcgctagttt tgaacttcta acagtccgtt tggctttctg
     7441 gttgacgaca ccgaggcgtt tcgttcccca tctcgctacc cacccgaggc tttcgtgtct
     7501 ttcccctgaa ttctcgttgt cataacttaa gattaaccgt tggcaagacg ataccctgac
     7561 cctgcgctat ggttgtagaa gcatgatcga cgttcccatg atccgatggt ttacgccgat
     7621 ttgaaataga cggctggctg gcccgcgggt ttttccctgg ccgccggact accgtgtcat
     7681 gaatcaaatt gttgttttta ccagtagttc gatactctcc accgcgcgag gggccgaagc
     7741 cgccctcgaa caagccgctc atcagttctc cccccggctg gcccgcggta tccgaatcgt
     7801 cgtcgaaacc aacgcttcgc acttgctggc tcggcttcgc tcccacgatg tcgatgtgct
     7861 tgtgatcgat gctcgtcaag aaacggcctc gcagacgccg agttcgagcc aagtgctgat
     7921 gagccagctt tttaccgaaa gcagtgcggg tgatctcatc gggcgtgagc aaacgtggct
     7981 catcgttccc cccaatcgat ggggcacgga gctttcgttc caagccggag ttttccgcct
     8041 cgcgggaatc cttgcgctcg aacaatccga ccaagcctgg cgtacgatct ggacaaacct
     8101 cgaaaaaacg ttgcgacgcc gtgaagcggg ccgggtggcg ttgtgtttgg ccggcggagg
     8161 attcgaaggt ctgtttttcg agttgggcgt gcttcgggcc ttgcagcact tcttacccga
     8221 gtacgcgttg caagatgccg atatcgtttg cggcatcagc gcaggggctg tgatcggcgc
     8281 gtttctcgcc aatggcttgt ccgctagcga catcatgagc ggaatgctcc gcgggcaagg
     8341 aaccctggat cgaatcgaac gctcgagcgt tttcgatctc aattatgcag aattatcctc
     8401 ccgcttcgga cgcgccgcgc gctcgctcgc acggcgccga acgactctgc ttcaatcctt
     8461 ttttcatctc gcacccaccg ggatcttcgc aggcaaacga ttgcgctcct acctgcaatt
     8521 gcaattgtca aagcccggtt tgaccgatga cttccgacaa ctcccccatg gcctgtacat
     8581 cggagccacc gaccaagata ccgccgaaca catcgtcttc ggaagccccg gctgggacca
     8641 cgttcccatt catcaggccg tccgcgcttc taccgccctc acacccatct tcgctcccga
     8701 actcatcgac aatcgatact tcgtcgacgg ggccttcacg cgcaccacca atgttcgtat
     8761 cgctgtcgaa aacggcgcaa ccctggtcat cctcgtcgat ccgttggtcc ccacccataa
     8821 cgatcagccc ggtcatgtct tcgaaaaagg cggcatcatg gtcggcatgc aagggctcaa
     8881 atcgctgatt catggtcgct tcgaccgagc catccacatg cttcgcgcca tgtatccgca
     8941 cgttgccttc cacttattcc aacccgatgg ggctacgatg cgcgtgatgg caggcgctcc
     9001 tgccaaaatt tttcatcgac cggaagtcga agaaatcgct tttcgagaaa ccctccgatc
     9061 cattcgtcaa tatcgattcg aagcattgca acgcgatttc acacgacacg ccatcgcctt
     9121 caccgaccca caaacagacc tgggaacgat caaacgcgat ctgctcgacc aggccgcaga
     9181 ggtccaagtg gcgtgaggtt tcgacgaatc atttccctcg tgcccagcga taccttcaat
     9241 ctcgcacgcc tcggactgct ccaccaactc gtcggacgta cgcgatactg cgttgtcccc
     9301 gacggtgtgc aacaaatacc tgtcatcggc ggaaccaaag acgtttcggt gcgcgacgtc
     9361 atcgccttgg ctccggatat cgtccttgcc aaccaagagg aaaatagtaa atccgatgtg
     9421 ctggcgcttc gtgccgcggg attgaacgtt cggttgactt ttcccaaaac cgtagaggac
     9481 ggctttcagg atttcgaagc gttggcagca tgtctcggcc tcgatagacc cgccatcgcg
     9541 cgacctgcgc cttgccccat gtccacccgc aaatgcttcg ttcccatctg gcgcttgcct
     9601 tggatgacca tcaacgggca aacctatatc ggtgatgtcc tccgatggct aggattagtc
     9661 aatgttttcg agcacttacc ttcagaggac gcacagggca aggacagtcg ctacgcacgt
     9721 ctgacgatgg atcaaatgct acaagctcag ccggatgttg tcttgctggg ggacgaccca
     9781 tacccgtttg ggtcggaaca tgtgcattat tttcggtcct tggacatccc cgcggcgcga
     9841 acgggagcca tctttcgggt gagcggacgt gatttgtgtt ggcctggagc gtgggccgaa
     9901 cacgggctcc tcaggttgcg aaaggtgatg agcgtgcgcg atgagccatg agtcgtcgtc
     9961 tcccggattc ggagcgctgg gctgaaaaac agaaagaaga atgcgagcaa agccgcaggc
    10021 aaagacctgg ttacgattcc gttttcgctt cttcttgtaa ctcgatgagc acacaaagcg
    10081 cttcgatttc acgcttcgtt tggatctcct tttgccggag gctgcgaact ttggcgtcca
    10141 atgccttgct ctcggcttca atctccaaaa gcttattttt gagtagctcg cgttcctttt
    10201 tcaatagttc cagtccaatc acgtccacct ccgtccgcta accgcggcgt cgttcctcga
    10261 ccatctcagc atataaggct tccgtttttt cggcgatatg ggtccaggaa aaatgatctt
    10321 ccacccgtct tcgcccattg cgcccgaact ttttcgccat ctccggattg cggagcaacg
    10381 tattgatggc atcggcaatg gcccggggat ttgctggttc caccaaataa cccgtttctc
    10441 catcgacaac cgtctccagg attcctccta cccgactcgc caccaccggt gtttcgcacg
    10501 ccatcgcttc gaggttgatg ataccgaagg gctcgtagac cgaggggcag gcaaataccg
    10561 acgcgtttgc gtacaattcc acgtattgct cttcgcgcaa caacgagttg atccacaacg
    10621 cccgaggttc tttggcgact ttttccgcaa tctcctcttc cacctccttg gtatccggcg
    10681 cgctcgtgca gcaaaccagc cgaacccccg gatccaccga cgccatcgca tcgatgagat
    10741 acgtcatgcc tttttgcctc gaggttcggc ctacgaacaa gatgtaggga ccatcgatat
    10801 cgaaggcctt gcgcgtgctg tccccatgca ccggacgata caagtccaga tctatcccgt
    10861 tatggataac ccggacccgt tcgggatcga cgttgaagtg atcgagtacg tcttttttcg
    10921 atccttgcga aacgctgatg atccgatcgg cgttttcgag ggccaccttt tcgacccagg
    10981 tggataagtg ataggactta ccaagctgtt cctctttcca agggcgaagc ggttcaaggc
    11041 tgtgtaccgt gcaaaccaac ggaatatcgt atagaacctt ggccatataa ccggccatgg
    11101 ccgcgtacca ggtatgtgta tgcacgacat ccgaatcgac atggtcacgg accagggata
    11161 agtccgtgga gaacgtgcca agagtcgagt tgaatcgacg gtccgtccct tcccacatac
    11221 gatcccaggc ctgatatccc tttacggtta gatttccatc ggacatgcgc tgctcgccga
    11281 agcaccgaac ctcgacttgc atccgcttgg caagctcgcg cgtgagaaac ttcacatgaa
    11341 cgccagcgcc tccatagaca tggggaggat actctttcga aatgatggtg accttcatcg
    11401 tccatctcct ccatcaatgg gccgcgccgc ggaccgtacg cccggatgcc acgggatcac
    11461 agagcaagtc cgaactggtt ttgggataaa tgatgcagtt ttttccaagc aaaccgcctg
    11521 ccggaacatg cacgtccatg ccgagtacgg tcgctccccc ctgcaaggaa ccaggaagct
    11581 cctcgttcgc cacgagattg cccatgtccg ctcccacccg tgcgcctcga ccaatcacgc
    11641 aacgcttgtc gcaaatcact ccatcgacca ctgccccgtc ttccacgact accttctccc
    11701 ataacacgct gttgcgaacg atggcaccgc gcccaattcg cacgtccgaa ctcaagaccg
    11761 aacgctcgac acgcccctga atgttgcatc ctcccccgat cagcgaatcg accacctccg
    11821 cgcccgaaaa acaacgtgtg ggcggaggcg gggccgcgcg acgcatcatc gtattggtac
    11881 gaacgttcca cgatgaaagg tcaatgcgtg gcgatggacc gagcatatcc atgtgcgccg
    11941 catgataggc gtcgagcgtg cgcgcatact cccattgacc ataatggacc caaccaaacg
    12001 ctctgcggtg cgccatcatg tgtggcagaa tttcatcgta aatttgatag gtcttctgct
    12061 cctcgggcgt cgcatgcgca tgacgccgta actcctccac gaggacttca cggcgaaata
    12121 cgtagacggt catcgaagcc aggttcgtcc tcggatgctt gggcttctcc atgtagccga
    12181 tgattcgccc ctccgcgttg agttcggcta tgccgaaacg cgaaggctct acatccatcg
    12241 gcgtaaacgc aagtgtcaca tcggcatcgc gctccatgtg gaaacgaagc agcggtgtgt
    12301 aatccatgct gtaagcatga tcgccggaca caatgagcac gtcatcaggg cgatgcaact
    12361 ccagaaaatc gatgttctga tagagggcgt cggccgtgcc gcgataccat tgagaatggt
    12421 caagtccaat atacggtggc agaaagcgca cgccccgctc gttacccacg aagtcccagg
    12481 gcaaaccaat acccacgtga tcgatgagcg atgatggacg gtattgggaa aggatgccga
    12541 cattgtcgat acccgcggta ttcgccaggt tggtcagcgc aaagtcgatc attcgatacg
    12601 cgccgccaaa cagcaccgct gattttgggc gccagaaggt caacactgac atctcctcgg
    12661 cgcgacctcc cgcgaacacg atggcgatgg tttttcgcat tcgaaaccct ccgtaattgg
    12721 ttgaccggca gtgtagacgg gcttgcgccc gaagccatct gcttccttga aatacctaaa
    12781 tttccctaaa aatttcgctt tgaaaaggga tgggggcgcg ttgagcgcaa ccgcttggcg
    12841 gccaacatgc ccccatgaca cgatatcaga cagactggcg ccgaaccgca acttgctcca
    12901 agctgccacc gtgtcttccc tttccccccg cctcattcac ctggcatcgc tgtggcagcg
    12961 cgcttgcggc ccatggcggg gacgactctt cgccgcctcc cttttgctca tcatcgtcgc
    13021 tgccgctcac ctcgctcgtc acggcaccat atcctctcgc ctcctcgctg ccgccatcgt
    13081 cgttgtgacg tgcgtcatcg ctgtggtcct gcggaaaatg gcacagcgcc aagcgcgtaa
    13141 tctcattcga ctggcgaaaa aactcggctc cctcgaacac ccttccctgg gcgaacgcgc
    13201 cgctcgcgcc gtgcgtctcc tcacgactac ccatggctcc gatgacgcgc tgagtgcggg
    13261 gctcgctcat ctgcacctcg aacgactgct cgctcagatt cgccccgaaa ccctgtggaa
    13321 agccgcaaaa aaacgtgccg atgtcgcaaa aatcgtagcg ctttccctcc tcgctctcgc
    13381 cctcggtgcc ttcgcttaca gccctcttcg tgtggcggaa ggtgtcgacg ttctgctcgc
    13441 gtaccgtggt cgcgcgccca tcgatatcga ttgggtcgaa tccgcggtcg tcgatattca
    13501 acccccggcc tatctcggcg aatcgatcca acacgcgatg gcgttcgacg agacctacca
    13561 acccgtggga acgaaactct cggttcacgc acggccgcgc tttgatggac gcacgctcgt
    13621 gcttaccgac ggaaaacggg aaaccccgtt cgtggatgat gcaaaagggg gaatcgtcgc
    13681 gacctggacc gtcgagcatg atgcaacact cgagatcgcc gctcgtttcg gcgatgtcct
    13741 catcgcccaa agcaccagcc tgcgcgtggt agccctcgaa gacgaggcac caaaggtgat
    13801 cgtcgacgat actccgagga ccgttcgttt gctcgacatc caagaagttc ccatccgcta
    13861 cgaaatcact gacgatcatg gcttgacgca cgtggaattg gtgttgcgta cggcaggtaa
    13921 ggaatcacga cgatcgctgg ccaaacatga tggtacgttg acgttcgatc gtggtgccac
    13981 ggtgttgcac cgtcaggatc gcgtgctcgt gcaagctcat acacccgtgg aagtcaccat
    14041 cgaagcgcgc gacaacaatg ccgtgggagg gccttcttgg gggaaaagtc agcccattgt
    14101 cgtgcttccc cccgcggttg gtgaagaaga agcattgcgt ttcgaagccg tgcaatccct
    14161 tcgaaatgac ttgctcgacc tgttggcgtc acgcctacga cgcgaagcgc cgagccaaca
    14221 cgaggatcgg gcggcgttga ataccaaaga aacaaaggcg catgcctctt tcgaacaagc
    14281 cgcaaatcag gtttcacagc agtcctacgg agggatcgtg gtgtctcgtc gcatgcgcgc
    14341 attcatccaa ggacaactcg aacgattgcg aaaggctacg gatggcgcat gccccacgct
    14401 ctccgaacag gcttgccgta gcggtcttag gcgattgcaa gacgtaaccg gtgatgtggc
    14461 gttggccctc gatgtggcta caagaacgct gtcgactcgt gattcgatcg ccattgcacg
    14521 aaaactggcg gatgtagcgg atgaggctgc ggagggcgcc agacagattc gccaatccga
    14581 atcgcaacga ggcagggttc gcctggatgc atccttggag gttttgcgtg gaggaagcgg
    14641 ttccatgctt cgccttggag gattgggagg cgacttgggc gaaatcgtgg ccatgggtgt
    14701 acgaaacatt caacggtgta tgcatcgtga cgacacccaa tgcgctgaat ccgcggcgtt
    14761 gcacttggc
//
LOCUS       DAOSOR010000063        14668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4130_length_14668_cov_4.360569, whole genome shotgun sequence.
ACCESSION   DAOSOR010000063 DAOSOR010000000
VERSION     DAOSOR010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 14668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14668
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4130_length_14668_cov_4.360569"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PK710_05245"
     CDS             <1..366
                     /locus_tag="PK710_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09153.1"
                     /translation="PAIIPPPNSTVPTIAEWDAAPWEITVSRSTPLGCETKMVREWLR
                     VSCRDENTDGGKVLGLSIEQKGGVQAYVYERKGVASLVVQVVKGKKHVTRFRWTDRTQ
                     RLVVDWTSLGPRPTIAFTD"
     gene            745..2052
                     /locus_tag="PK710_05250"
     CDS             745..2052
                     /locus_tag="PK710_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT09154.1"
                     /translation="MSKPSAILTFPDSCESEKWEPRSTRINLFDVGDWVAGLYEVRSL
                     LGSGGMGQVFEAQDHALDRRVALKACWPDMPAGALHQEARALAAFRHEALPIVHGIGI
                     HRRTEYIVMERIYGVTLEEHLHQRQRVGESFSSEEVLWLARKVAEGLAVVHVAGIAHR
                     DVKPANIMLSPDRRVVLMDFGLMLPEVVMQQQKEIAGSPAYMAPEAIDNDLTPGAGKL
                     LDVFGLGVTIFELLTARLPFDGSTLDEIREQQSRGAPDIRTFRPDIEQGFAALITQML
                     SPVADDRPDNAETLVWRFRDLQQRKPISQGPLRVLVVDDDPALTKLVEMIVRSVVPRV
                     EVAAVNDAESAVKRLREDPPDAMFLDLHMPRMNGVEVCMYMRGAGIARRTTVFAMSAG
                     AQDDDQQLLHHLGVRDFIPKGRDLRSQITSVLARTFPDHIQRV"
     gene            complement(2049..3701)
                     /locus_tag="PK710_05255"
     CDS             complement(2049..3701)
                     /locus_tag="PK710_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09155.1"
                     /translation="MWFALWKAIRLAKEKAFSRRLASLWRWDLPLSLLILAILSYSWM
                     FSSISVPNERSRLYLTVALIDHHTLTIDEPVRRFGKINDWARYEDHYYTDKAPGSSLL
                     GAVVYGLVRCFSNPQDWKIHELINLMRTCVMLPLGLIGFLWLRRLLRRLGLDPPSTDI
                     TAVGWIVGTSAFHYSTAFYGHQIVAVCMIGALDLILMAEQRLADSLPASSALGPAWGA
                     GMMAGLAGLTEYQAGIPTACLLVYVVFGPMRRHGSLWLAVALGAFPFMVMLFGYNTLA
                     FGGPFELSYHHLIDPSLRELHGKGIGGVAAPQWQYAYGGLFSLHRGLFTTSPVFGLAV
                     PGLIIMVLRRRVRLAILVGMTWLYFLLFISSTQIWFAGWSFGPRLLVPVMGWVMIPIA
                     YCFHGMGKIPVLDGLGRGLLIVGILYHQTVHAVFPELPESATHPFKDVIVPAWRAGVV
                     SPNLCTKWIGWKGFASLLPLAVLLMLAVVIVVLWPKMGSSRKTMACRAATALGTTLLC
                     CVPLLLIKPGWSERESQRFIQWMEQLIAEEQRLSGKQLTASP"
     gene            complement(3806..4696)
                     /locus_tag="PK710_05260"
     CDS             complement(3806..4696)
                     /locus_tag="PK710_05260"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT09156.1"
                     /translation="MDDSRPTWPGVTRRSSSSSLRAKRSLSLFLEGEEFVLDDGEYLL
                     GRSPSCDIVVDDMLVSRVHARISVTEGMVVLRDAGSTNGVYVNAARIDLPTPLRDGDR
                     ILVGTKELSVFAVRVGSSSRSSRPVVTNSSIPPPSHNPTFELTEDDDDRGFSPTEKTE
                     AFTQLSILARSMMDIGETDGAIHLLSGYFDKMLHAAREGRTVSVGIVEAACRFAMEFA
                     EAKHDPKWVDYVLEIHLNLRKPMTADLIDDLCKLRRKEIRYDPDLLLFYKAALQQTEP
                     SLPLSLRVLCARIMTLDDEA"
     gene            complement(4747..6276)
                     /locus_tag="PK710_05265"
     CDS             complement(4747..6276)
                     /locus_tag="PK710_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOT09157.1"
                     /translation="MNESAACHGVVRLARRRFTSLVDCANSPGKEKEAVGASGWATVD
                     VLSASDRRRKQPWETSMKHEAKARIIKRIRIRIGDMPECLGRFAMLVGEHRGRFGQIT
                     TVQLGKKAKIRDVDIHTPDEASFDRLCDAIRKMDGIDLLATQDVVLEMHRKGKIEVTS
                     RVPVESIDDLSIVYTPGVASVCNKIHEKPELALEYTSIPNNVAIVTNGTAILGLGDIG
                     PVAGMPVMEGKALLFRVLADINGYPILIDSKDPEVIIQTVRAIAPTFGAIKLEDIRAP
                     ECFEIEERLDAALDIPVMHDDQHGTAVVVLAALLNIAASTYINLKRSAVGIVGLGAAG
                     SGIFRLLRAYGVEQVYGADINEVMTSRFESFGGIPESLEGVMAKSKIVIATTGVPGLI
                     KPEMVQPQQVVLALSNPDPEIDPDEAVQAGAVFAMDGKSVNNAMAFPGLFKGALQARA
                     TKINDAMRIAAAKKIAEFATETELIPNILDKRVHEAVAQAVEKAAFKTGVTRVLDSVE
                     F"
     gene            6413..7087
                     /locus_tag="PK710_05270"
     CDS             6413..7087
                     /locus_tag="PK710_05270"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT09158.1"
                     /translation="MEPVQGFDVQRMPLGLTARELAQDEMILRAAIEQDPTNVQNHLT
                     LGKTYYWHGQIDEAIDSFQRALQIDDRDPAAFYHLGICHFRAARFTQAEKALAKAIAL
                     HPRFVMAHYWLGITAYHQGKYEEALGCFETICRESPESMIAHYHAALACMSMGNWALV
                     RQHLEILRENGNTGTRVFLYLGRAYRRMNRPTEAIQVLRKGLSLYPRDELLKEALAEL
                     TDVQDP"
     gene            complement(7111..8535)
                     /locus_tag="PK710_05275"
     CDS             complement(7111..8535)
                     /locus_tag="PK710_05275"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT09159.1"
                     /translation="MKTPRVTLVNPPATYAQQIPQKCFPPMGLLYLAASLESAGAQVD
                     VMDANAAGLSHDACAKRVEAFAPDLVGMPIYAETAESCARCLATIRNRCPHIPIVAGG
                     PQATVSPSWVFREIPSVDFVLAGEAERSIVSLARAIVDDALRCTTIPGLWTRSSTTCL
                     PVENINKLDSLPLPARHRLEDLYARHRYYALLVADRHIDCLITSRGCPHQCKFCSNAT
                     NRIRYRGIDACLDELERLRSRGVRTVEILDDNFTASEARAFELLDRIRLQRFGLALRI
                     KSRADAVTEKLMAACRRAGVYQVSLGAESGSASLLKAMNKRITPAQIRRAVRLILDEG
                     MACHTSWIVGYPGETRQTIAQTADLIIELKPTTAGVSILIPYPGTDVYEQAKRQGTLE
                     GDWSASSSEVPWIRLPWTNGREDLVAAKRALMRRVYFRPHYVAEFGKRIVREANVTLA
                     RYAVQELKRSLTIGISSFACKAWR"
     gene            complement(8532..9695)
                     /locus_tag="PK710_05280"
     CDS             complement(8532..9695)
                     /locus_tag="PK710_05280"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOT09160.1"
                     /translation="MAVRRCSSYDDCDGVLSQLLTDTGLSDVLRNSRVLLKPNMMKAT
                     AADRADATHPSIVASLTRVLMAMGCEVWVGDSSGLLGLTAEVLEGSGMAPAVRAVGGK
                     TINLDAGPFEKISSTGSATHEIWVPRILFDVQHVVQVPKLKTHSLTGMSCSIKNLMGL
                     LPGATKCALHVRRPSALPLSHALLDLAETLVRKGVHLSGTVVDAVWAQERKGASEGPR
                     RRDLGLIIASRDLFATDLVCSQLIDADRHSLSTVLAAFQRRLGPCHLEDVEIVGDRNA
                     IHAAVPLESAVAGWKDKYWPVHTGHYWFRERIVRPWHDPRRCHGTRACIDVCPTDAMR
                     IVGKRIEITSQCIRCFACRAACPHGAIKLEVPFGMRAIFSRRAEGIDLSKVLR"
     gene            complement(9804..10757)
                     /locus_tag="PK710_05285"
     CDS             complement(9804..10757)
                     /locus_tag="PK710_05285"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOT09161.1"
                     /translation="MMRKPSCLLSILLLSSFGLGACSSDNDPQCSAAPSCVSPEASTY
                     DSQVDPTDSSYETSIEASPDTDTSEGGEAASGSDAPAESALPEASSPDGLRFMTFNIQ
                     HGANASLDAIAEAIRNENPDIVGLQEVDVDAQRSGHVDQPRVLSQRTGMAAAFQFSLT
                     FSSGGYYGLAVLSRFPIVSTSKRLLSSTGEQRILMRVDVLTEHALLPVGVTHLGLTAG
                     ERKTQVSEILEDLGSEGFAVLMGDMNAQPGDEEMKSLASAMNDAWLESSGQGFTFPVD
                     TPTKRIDYIFLGNNWPSPTQAHVPTTKTSDHRPVVVFVPWN"
     gene            10867..11904
                     /locus_tag="PK710_05290"
     CDS             10867..11904
                     /locus_tag="PK710_05290"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOT09162.1"
                     /translation="MNDKSEGAPRRDIVRSPRPWAGFATSIVLSIAILLWLWHRHALV
                     WTSLKTRWATADHALLVAVFGGSAAFHIAAGAFKLHRVLRSMGVDIRWNETLRVRLGA
                     GPVRAFLPFSTGEIIHVLYFRARKRMTVADTSGIMIFDKSMNLIGSLWWLALGIFLAS
                     EPRIQLGILRQASSVPVALIAALLAVSFIIVFIPSIHRHVADAAMIIHPRVGEIVSGM
                     LSPFRQLHTVQKIELLIYGVVFQLRPIVVCFFLLRSLAVPVRWVDTLLAASAAVCAGY
                     LPGFVVGSGPRETVLVEAFGAAYGPVEAIFLGGLLMTFAVNVFPALLGLPWTVWFLRR
                     LRRSDVTGRKG"
     gene            11901..12482
                     /locus_tag="PK710_05295"
     CDS             11901..12482
                     /locus_tag="PK710_05295"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT09163.1"
                     /translation="MNLRARAALGRAVLRYRFLGIRTPIAIAWLVTGRCTACRSYCRW
                     KSERDTNELGTTAALELIEEMAEAGVLMISFTGGEPLLRDDIGLLIHRVKSKGIVCKL
                     NTNGDLVLRRIDSLRSLDLLQISLDGPAKLHDPLRGRGSADRASNAVRRAHQEGIPVQ
                     VITCLTRYNLDRLDEVLAYAKALDVRLHVQPLA"
     gene            12531..12917
                     /locus_tag="PK710_05300"
     CDS             12531..12917
                     /locus_tag="PK710_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09164.1"
                     /translation="MDSLIRTRKQRHHLADYLATTSSELRHYRAMYSQGLRGCHCAVV
                     TATLLPDGSMIFCSSGREFTAYDTKTLGFSGAFAKLRPPICDGCTCVGKLRISKVCDL
                     DPAVIWDLVVQRGLKASQQGKKGRWD"
     gene            12908..13324
                     /locus_tag="PK710_05305"
     CDS             12908..13324
                     /locus_tag="PK710_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09165.1"
                     /translation="MGLIALDLNPTPKQVRIFGSVGIPLTLGILGLAGGTHFESSSNA
                     LFLGVLAVGAIGSSWLYPPVIRILLLGFRLSTYPIALVFAHITLAAFFFLVLTPLGLL
                     VRWLHGDPLSRRWDPSSSSYWTACTSSAPRRYLRPY"
     gene            13396..13590
                     /locus_tag="PK710_05310"
     CDS             13396..13590
                     /locus_tag="PK710_05310"
                     /inference="COORDINATES: protein motif:HMM:NF039953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HOT09166.1"
                     /translation="MSDPNNDFEALSRQSRGGFLSDVWDLLRHSKRWWLTPIVFILVL
                     FGAILALGASGAAPFLYTLF"
     gene            13704..>14668
                     /locus_tag="PK710_05315"
     CDS             13704..>14668
                     /locus_tag="PK710_05315"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT09167.1"
                     /translation="MIKTIEIPTEKGPIRPDDRFGFVLAPGDYDGTHINRIGLRGPEI
                     EEHKRAECIRILMLGGSTTYGNTVSTEEAYPAVVQHLLRQTNPHRCIEVINAGVSGAY
                     SYHHLVRYQHLYSALRPDIVTMYIGWNDYAGYLWQRGDWKADSLAVNSLIIDVGSIPM
                     ALLRRSSAARIAYTSYKRASFRQALSELSSCTDVAAALRPATEGLHHNLARLIELVQR
                     DGAKVLLIKFPYVLRDDQIKQDLELLSTMDVPGRIESMLPMVRFEPSMPTLAASVMDR
                     LAQEKRVGVVDCRTVFYDLSLEERLDRFDDAIHPNAKGYALIGKCV"
BASE COUNT         3236 a         4185 c         4006 g         3241 t
ORIGIN      
        1 cccgccatca ttccgcctcc taattccacc gtgccgacca tcgcggaatg ggatgccgcc
       61 ccttgggaaa tcaccgtctc ccgatccacg ccgctaggat gtgaaaccaa aatggttcgc
      121 gaatggcttc gtgtctcctg ccgcgacgag aacacggatg gagggaaagt gctaggtctt
      181 tcgatcgagc aaaaaggtgg cgtacaggcc tatgtctacg agcgcaaggg agtggccagt
      241 ttggttgtgc aagtggtcaa aggcaaaaaa cacgtgacgc gctttcgttg gaccgatcgc
      301 acgcaacggc tggtggtgga ttggacgagc ctcggtcccc ggcctacgat cgcttttacg
      361 gattgagcac caaacctgta acacaccgtt tttattatat ttttatcact ccaacccatc
      421 gatcggtggt tccctatcct gcatacgcaa cgggcacccg ccgcgtgatg agattgacta
      481 ctttctatcg acatgctcgt tcatcggacg gtcgaaatca tcgcttcgct ttgtcgtaag
      541 aaacgaactc cagcgcccaa cccgccgaac ctcactcatc tttcacgttc ttcgccactc
      601 gttcatgccg aacgtcctag ggcaacctca ccggatgacc cttggcaata ccgttatcag
      661 accccgtcct tgcctcatca ggacatcacc catccttgcc gatggtcttt cccacggaaa
      721 acacggtaca attcgagggc gctcatgagc aaaccttcag cgattctgac cttccctgac
      781 tcgtgcgaat cggaaaagtg ggaaccacgt tcgacgcgta tcaacctctt cgatgtggga
      841 gattgggtgg caggattgta cgaagttcga tcgctgcttg gctcgggtgg catgggacag
      901 gtattcgaag cgcaggacca tgccttggat cgtcgtgtgg ccctcaaagc ctgttggccc
      961 gacatgcctg caggagcgtt gcatcaagaa gctcgcgcgc ttgccgcctt tcgccatgaa
     1021 gcgcttccta tcgtccatgg catcgggatc caccgaagaa ccgagtatat cgtcatggaa
     1081 cggatttatg gcgtgacgct cgaggaacat cttcaccaac ggcagcgcgt aggcgagtcg
     1141 ttttcctcgg aagaggtatt gtggttggcg cgaaaagttg ccgaagggct tgctgtcgtt
     1201 catgtggcgg gcattgccca tcgagatgtc aaacccgcaa acatcatgtt atcgccggac
     1261 cgccgtgtcg tgctgatgga cttcggactg atgctgccag aagtcgtgat gcagcaacaa
     1321 aaagaaattg ccggatcgcc tgcctacatg gctcccgaag ccatcgacaa cgacttgacg
     1381 ccgggcgcgg gcaagctgct cgatgtattc ggtttgggtg tcaccatctt cgaactgcta
     1441 accgcacgat tgcctttcga tgggtccaca ctcgatgaaa tccgcgaaca gcaatcccgg
     1501 ggggctcctg atattcgaac ctttcgtccc gatatcgagc aggggtttgc cgcgctcatc
     1561 acgcaaatgc tctctcctgt tgctgacgat cgccccgaca acgcagagac cttggtttgg
     1621 cgatttcgcg acttgcaaca acgaaaaccc atcagtcagg gaccgcttcg ggtgttggtc
     1681 gtcgacgatg atcccgccct taccaaactc gtggaaatga ttgtgcgatc cgtggtcccc
     1741 cgcgtggagg tagcagcggt gaatgacgcg gaatcagcgg tcaaacggct tcgggaggat
     1801 cctcccgacg cgatgttttt ggacttgcac atgcctcgta tgaatggcgt cgaagtgtgc
     1861 atgtacatgc gcggcgcggg aattgcccga agaaccaccg tttttgccat gagcgcgggc
     1921 gcgcaagatg atgatcaaca actgttgcat catctcggcg ttcgagactt cattcccaaa
     1981 ggccgcgact tgcgttctca aatcacttcc gttcttgcgc gaacctttcc ggatcatatt
     2041 cagcgagtct aggggctcgc cgtaagttgt ttgccggaca gtcgctgctc ttcggcgatg
     2101 agttgctcca tccactgaat gaatcgttgg gattctcgct cggaccaccc cggtttgatc
     2161 aggagcagcg gaacacagca caacaacgtc gttccgagcg cagtggctgc cctgcatgcc
     2221 atcgtttttc gcgacgaccc cattttcggc caaagcacaa cgataacgac cgccagcatg
     2281 agcagtacgg ccaagggcaa caggctcgca aagcccttcc atccgatcca tttcgtgcag
     2341 agattcggag aaacgacccc cgctcgccat gccggaacaa tcacatcttt gaacggatgc
     2401 gtagcgcttt ccggaagctc aggaaatacc gcatgaacgg tttgatggta gaggattccc
     2461 acaatcaaaa gccctctccc taacccgtcg agaacaggga tcttgcccat cccatgaaag
     2521 caataggcaa tgggaatcat cacccagccc atgactggca cgagcaatcg aggaccgaaa
     2581 gaccaacccg caaaccatat ttgggtactc gatatgaaca gcaaaaaata gagccacgtc
     2641 attcccacca agatggccaa tcgaactcgt cgtcgaagaa ccatgatgat gagacctggc
     2701 acagcaagtc caaatacggg tgaagtggta aacagacctc gatgcaagct gaacaatccg
     2761 ccgtacgcgt attgccattg cggagcagcc acgccaccga ttcctttgcc gtgtagctct
     2821 cgcaacgacg ggtcgatcag atggtggtac gacaattcaa acgggccacc gaaagccaga
     2881 gtgttgtatc caaacagcat aaccatgaag ggaaacgcgc ccaaggccac ggccagccat
     2941 agcgaaccat gccgacgcat cggtccaaag acaacgtaca cgagcaggca tgcggttggg
     3001 attcccgctt gatattcggt aagaccggcc agtccggcca tcatccccgc gccccaagcc
     3061 ggccccaggg cgctggaagc gggcaacgaa tccgccaggc gttgctcggc catcaagatg
     3121 agatcgagag cgccaatcat acagacggca acgatctgat ggccataaaa cgctgtgctg
     3181 taatgaaagg cggacgtgcc aacgatccac ccgacggcag tgatatccgt ggatggtggg
     3241 tccaggccca atcgtctcaa aagcctgcgc aaccaaagaa aaccgatcag cccaaggggc
     3301 aacatgacgc atgtgcgcat caagttgatg agttcatgga tcttccaatc ttgcggattc
     3361 gagaagcatc ggacgagccc gtacaccact gccccaagca agctcgaacc aggtgctttg
     3421 tcggtgtaat agtgatcctc gtatcttgcc caatcgttga tttttccaaa gcgacggacg
     3481 ggttcatcga tggtgagggt atgatggtcg atcaacgcca cggtgaggta caggcgggat
     3541 cgttcgttgg gcacggagat ggaactgaac atccatgagt aactgagaat ggcaaggatg
     3601 agcagagaaa ggggtagatc ccatcgccat aacgaggcga ggcgcctgga aaaagccttt
     3661 tctttggcga gccgaattgc tttccaaagc gcaaaccaca tgaggggcat cctagcagca
     3721 tgtgctttgg ctgatcgagg ctccgcagga caaagcgaag aacggatttc gctacggata
     3781 gcgcccgaag gcggaaaccc tcgcctcaag cttcatcgtc caaagtcata atccgtgcac
     3841 aaagcacacg caacgacagg ggtaagctcg gttccgtctg ctgtaatgcc gctttgtaaa
     3901 acaacaacaa atccggatcg tagcgaatct cttttcgacg cagtttacac agatcatcga
     3961 tgaggtcggc agtcatgggt tttcgaaggt tgagatggat ttcgaggacg taatccaccc
     4021 atttgggatc gtgtttggcc tccgcgaatt ccatggcgaa gcgacaagcc gcctctacga
     4081 tgccgacact cacggttcgt ccttcccttg ccgcatgcaa catcttgtcg aaatacccgg
     4141 acaataaatg aatcgcccca tcggtttctc cgatgtccat catgcttcgc gcaagaatcg
     4201 aaagttgggt aaacgcttct gttttttccg tgggagaaaa accgcgatcg tcgtcgtcct
     4261 ctgtaagctc gaacgtaggg ttgtgcgaag gcggagggat agagctattc gtaacaaccg
     4321 gcctggaact tcgggacgag ctgcctacac gcacggcaaa cacggacaat tctttcgttc
     4381 ccaccaagat gcgatcaccg tctcgcaacg gcgttggcaa gtcgatccga gcggcattta
     4441 cataaacgcc attggtgctt cccgcatccc gaagcacgac catgccttcg gttacggaaa
     4501 ttctcgcatg aaccctcgac acgagcatat cgtcgacgac gatgtcgcac gaaggactac
     4561 gtccgagcag gtattcaccg tcgtcgagaa cgaattcttc gccttccaga aacagcgaga
     4621 gagatcgttt ggcccgaagc gaagagctag acgatcttcg cgtaacaccg ggccaagtcg
     4681 gtcgagaatc atccacgcca caagtgtacg tcacgagcga aaaaaacgcg aacttcgggg
     4741 cgatgattag aattccacgg aatccaagac tcgcgtcaca ccggttttga aagccgcttt
     4801 ttcgaccgcc tgggccaccg cttcatgaac acgtttgtcc aagatattcg gaatgagttc
     4861 cgtttccgtg gcaaattccg caattttttt ggccgcggcg attcgcatgg catcgttgat
     4921 tttcgtggct cgggcttgca aagccccttt gaacagtccg gggaaagcca tggcattgtt
     4981 gacggactta ccgtccattg cgaaaaccgc acccgcttgt accgcttcgt ccgggtcgat
     5041 ctcgggatcg ggattcgaca acgcaaggac cacttgttgg ggttgcacca tctctggctt
     5101 gatcaagcct ggcacgccgg tggtggcgat gacgatcttg gattttgcca tgacaccctc
     5161 gaggctctcg ggaatgcctc caaaggattc gaagcgcgat gtcatcacct cgttgatatc
     5221 ggcgccgtag acctgctcca caccgtaggc gcgtaaaagg cgaaagatgc cacttcccgc
     5281 ggcaccaagc ccaacgattc ccaccgcaga acgtttgaga ttgatatagg tgctcgccgc
     5341 aatgttgagc aacgcggcca acacgacgac ggcggtgcca tgctggtcgt catgcatgac
     5401 cgggatatcc agagccgcat cgagccgttc ctcgatctcg aagcattccg gagcgcgaat
     5461 atcctccaac ttgatggcac caaacgtcgg agcgatggct cggacggttt ggatgatgac
     5521 ttccgggtcc ttgctgtcga tcaagatagg atagccgttg atatccgcaa gcacccgaaa
     5581 cagaagggct ttgccttcca tgacgggcat tcccgccacc gggccgatgt cacccaagcc
     5641 gaggatcgcc gtgccattgg tcacaatcgc cacgttgttg gggatagacg tatattcgag
     5701 ggccaattcc ggtttttcgt gaatcttgtt gcacaccgag gccactcccg gggtgtacac
     5761 gatgctcaag tcatcgattg actccaccgg caccctcgac gtcacctcaa tttttccttt
     5821 tcgatgcatt tccagcacga cgtcttgtgt tgccaataag tcgatcccat ccatttttcg
     5881 aatggcatcg caaagacgat cgaaggaagc ctcatcgggg gtgtggatat cgacgtctcg
     5941 tatcttggct ttttttccaa gctgtaccgt cgtgatctgc ccgaaccgtc cgcgatgctc
     6001 ccccacgagc atggcaaacc ttcccaggca ctctggcata tcgccaattc gaatgcgtat
     6061 ccgcttgatg atgcgagctt tcgcttcgtg cttcatcgac gtctcccatg gctgttttcg
     6121 tcgtctgtcc gatgccgaaa gcacatccac ggttgcccaa cccgatgcgc ccacggcctc
     6181 tttttccttg cccggggagt tggcacaatc gaccagcgag gtaaagcgtc gccgcgcgag
     6241 acgcacaacg ccatggcaag cggccgattc gttcacgtca agtgtacgcc aaccttgcaa
     6301 agcatttctc taggcatgtc cgaggagccg acagcgccga ccgcttgacg aacctcggtg
     6361 ggcgcccaat ccaacactgc gaacgacagt atgtattgga aagggccata ccatggaacc
     6421 ggtacagggg tttgatgtcc agcgaatgcc tctcggattg accgcacggg agttggcgca
     6481 agacgaaatg attctgagag ccgcgatcga acaagatccc accaatgttc agaatcacct
     6541 gacgcttggg aaaacctatt attggcacgg tcaaatcgac gaggccatcg actcgtttca
     6601 gcgggcatta caaatcgacg accgcgatcc ggccgcgttt taccacctgg gaatctgcca
     6661 ttttcgcgcg gctcgattca cccaagcaga aaaagccctc gccaaagcga ttgccttgca
     6721 ccctcgtttt gtcatggcgc actattggct cggtatcacc gcgtatcacc aaggaaaata
     6781 tgaagaggcg cttggttgtt tcgagacaat ctgccgggaa agccccgagt cgatgatcgc
     6841 tcactaccac gcagccctcg cgtgcatgtc tatgggcaac tgggctctgg tccgacaaca
     6901 cctggagatc cttagggaaa acggtaatac gggcacgcga gtgtttctct atctcggtcg
     6961 tgcctatcgg cgtatgaatc gcccgacgga agccatccag gtgctgcgaa aaggattgtc
     7021 cctatatcca cgagacgaat tgctcaagga agccttggcc gagctgacgg atgtccagga
     7081 cccctagccg ttgcgcgacc gtccaaccat tcatctccat gctttgcatg cgaacgatga
     7141 tatcccgatg gttagcgacc gtttcagctc ttgcaccgca tatctcgcta gcgtgacatt
     7201 cgcttcccgc acgatccgtt tcccgaactc agcaacataa tgtggacgaa aatagactcg
     7261 tcgcatcaag gctcgcttcg ccgccaccaa atcctcacgg ccattcgtcc acggtaatct
     7321 tatccaggga acttccgaag atgacgcgga ccaatcgccc tcgagcgtgc cttgccgctt
     7381 ggcctgttcg tatacatcgg ttcccgggta aggaatcaag atggaaaccc cggcggtcgt
     7441 cggctttagt tcgataatga gatccgccgt ttgagcgatg gtttgccgtg tttccccggg
     7501 ataccccacg atccagctcg tgtgacaagc cattccttca tcgagtatca gacggacagc
     7561 gcgacggatt tgcgctggcg tgattctttt gttcatggct ttcagcaggg acgccgaacc
     7621 cgattcagcg ccaagactga cctgatacac tcctgctcga cgacaggccg ccatcaactt
     7681 ctccgtgacg gcatcggcgc gagacttgat tcgaagggcc aatccgaaac gttgcaggcg
     7741 aattcgatcg agtagctcga acgcacgagc ctccgacgcc gtgaagttgt catcgaggat
     7801 ctcgacggtt cgaacgcccc tggagcgaag ccgctccagt tcatcgagac aggcatcgat
     7861 gccgcgataa cgaatacgat tcgtcgcatt cgaacaaaac ttgcattgat ggggacaacc
     7921 gcggctcgtg atcaagcaat cgatgtgtcg atcggcaacg agcaaagcgt agtaccgatg
     7981 ccgcgcatag agatcttcga gccgatgacg agcgggcaat ggtaagctat cgagcttatt
     8041 gatattttct acgggaaggc acgtggtcga cgatcttgtc caaagcccag gaatggtcgt
     8101 gcaacgcaaa gcatcgtcga cgatcgcgcg cgcaagcgac acgatcgagc gctctgcttc
     8161 gccagcgaga acgaagtcca ccgacggtat ttctcgaaac acccatgatg gagataccgt
     8221 ggcttgcggg cctccagcga caatcgggat atgcggacaa cgatttcgga tagtggccaa
     8281 acatcgggca cagctttcgg ctgtttccgc gtagatcggc atccccacca ggtcgggggc
     8341 gaaggcttcc acacgctttg cgcaggcatc gtgcgacagc cccgccgcgt tagcatccat
     8401 gacgtcgacc tgcgcacctg ccgattcgag gctggctgcg agatacaaca gccccatggg
     8461 aggaaagcac ttctgtggga tctgctgcgc ataggtggct ggaggattca caagggtcac
     8521 acgaggcgtt ttcatcgaag aaccttggaa agatcgatac cttcagcacg acgagaaaaa
     8581 atcgctcgca ttccgaaagg cacctcgagc ttgatcgccc catgagggca agccgcgcgg
     8641 cacgcaaagc aacgaatgca ttgtgaggta atttcgatac gtttaccgac gatacgcatg
     8701 gcgtcggtag gacaaacgtc gatgcacgct cgcgtaccgt ggcatctccg tggatcgtgc
     8761 caaggcctca cgatccgttc gcgaaaccaa taatggcccg tgtggacggg ccagtacttg
     8821 tctttccacc cagccaccgc ggactccagg gggacagcag cgtggattgc attcctgtcg
     8881 cctacgattt ccacatcctc cagatgacaa ggccccaaac gtcgctgaaa agcggcgaga
     8941 acggtggaca gactgtgacg atcggcgtcg atcaattggc tacaaacgag atccgtggcg
     9001 aatagatcac gcgaggcgat gatgagacca agatcccgcc gtcgtggccc ctcgctcgcg
     9061 ccttttcgct cttgtgccca cacggcatcg accacggtgc ccgacaagtg aactcctttg
     9121 cgtaccaagg tctccgcgag gtccagcagc gcatgcgaca atggcaacgc ggaaggacga
     9181 cgaacgtgaa gggcgcattt ggtcgcgccg ggcaacaacc ccatcaagtt tttgatggag
     9241 cacgacatcc ccgtcagcga gtgtgttttg agcttgggaa cctgcacgac gtgctgcacg
     9301 tcgaacagaa tgcggggcac ccagatttca tgagttgccg atcctgttga gctgatcttc
     9361 tcgaaaggcc ccgcatccag gttgatcgtt ttgcctccca cggcacgaac cgccggggcc
     9421 atacctgacc cttcgagcac ttccgccgta agccccagca atcccgatga atcccccacc
     9481 catacttcgc accccatcgc catcaacacg cgggtcagac ttgccacgat cgacggatgg
     9541 gtagcatccg ctcgatcggc agcggttgcc ttcatcatat tcggcttcag cagcacgcga
     9601 ctgttacgca gaacatcaga aagccccgtg tccgtcagca gttgactcaa cacgccgtcg
     9661 caatcgtcgt acgacgagca tcgtcgtaca gccacgcgga aaggcgatgt tggcgaatcc
     9721 tgcgtcatcc atcttgttgc tatcatgcga tgaattgcgg tgcaattcgc cctacgaagc
     9781 cgcgtcgccc gcagcgttcg ctttcagttc cacggcacga agacgacgac cggcctatga
     9841 tcggaggttt tcgtggtcgg aacatgcgct tgcgtagggg acggccagtt gtttcccaga
     9901 aagatataat cgattcgctt ggtgggggta tccacgggaa atgtgaagcc ttggccactg
     9961 gattcgagcc atgcatcgtt cattgccgaa gccagagact tcatttcctc atcacctggt
    10021 tgcgcgttca tgtcgcccat gagcactgcg aatccctccg acccgaggtc ctcgaggatc
    10081 tcgctgacct gagtttttcg ttcgcctgcc gtaagaccaa gatgcgtcac ccccacgggc
    10141 agcaaggcat gctctgtcag cacatcgacg cgcatgagga tacgctgctc gccagtggat
    10201 gacagcaagc gtttgctggt cgacacgatg ggaaaacggc tgagaacggc aaggccatag
    10261 tagccaccgc tcgaaaacgt gagggaaaat tgaaaagcag ccgccattcc cgtacgctgg
    10321 gacagcacgc gaggctggtc gacatggccg cttcgttgcg catcgacatc gacttcttgc
    10381 agtccgacga tgtccggatt ctcatttcga atcgcttccg caatcgcatc gagactcgcg
    10441 ttagccccat gttggatatt gaacgtcatg aagcgcagcc catcgggtga tgaggcttcc
    10501 ggcaaggctg actccgcggg agcgtctgat cccgacgcgg cttcgcctcc ctccgaggta
    10561 tccgtatccg gacttgcttc tatcgacgtt tcatacgatg aatccgttgg atccacctgc
    10621 gaatcgtatg tacttgcttc gggagacacg cacgagggag ctgcggaaca ttggggatcg
    10681 ttatccgagg agcaagcgcc taagccaaac gatgacagaa gcaagatgga aagcaggcac
    10741 gaaggttttc gcatcatgtt ttcatcatgc ctcgctttga tgtgaggtca atacaaggat
    10801 acgagccata cctacgaccg aacggcgatg gtatgctggg ccatgccctc cagggatcgc
    10861 ccggatatga atgataagag cgaaggtgcg ccacggaggg atatcgtccg ttccccaagg
    10921 ccgtgggctg gatttgccac gagcatcgtt ttgtcgatcg cgattttgtt gtggctgtgg
    10981 catcgccatg cgctggtctg gacatcgctg aagacgcgct gggcaacggc ggatcatgcg
    11041 ttattggttg ccgtattcgg aggttccgca gcgtttcaca ttgcggctgg tgccttcaag
    11101 ctacatcgag ttctccgttc gatgggcgtt gacatacggt ggaatgagac attgagggta
    11161 cgcctcggcg cgggtccggt tcgcgcattc ctcccctttt ccaccggtga aatcatccac
    11221 gtgctgtatt ttcgcgcccg gaagcgaatg accgtggccg acacatcagg gattatgatc
    11281 ttcgacaaga gcatgaacct gatcggatct ttgtggtggt tggcgctagg aattttcctc
    11341 gcctccgaac ctcggattca gctcggaatc ttacggcaag cttcatcagt tcccgtagca
    11401 ttgatagcgg cgttgcttgc tgtcagcttc atcatcgttt tcattccttc catccatcgc
    11461 catgtcgcgg atgcagccat gatcattcac ccgcgggtgg gcgagatcgt atccggaatg
    11521 ctttctcctt ttcgtcagct ccacaccgtg caaaaaatcg agttattgat ttacggcgtt
    11581 gtgtttcagc tacgtcctat tgtcgtctgc ttttttttgc ttcgaagtct tgctgtgccg
    11641 gttcggtggg tggatacgtt gcttgccgcg agcgctgcgg tttgcgccgg gtatttgccg
    11701 ggatttgtcg tgggatcagg acctcgggaa actgtgctcg tcgaagcttt cggcgcggct
    11761 tacggtcccg tggaggcaat atttttgggg ggactgctga tgactttcgc ggtcaatgtc
    11821 ttccctgccc tgctcggcct tccttggaca gtctggttcc tgcggcgctt gcgacgctct
    11881 gatgtgacag ggaggaaggg gtgaatctgc gagcacgagc ggctttgggt cgtgctgtct
    11941 tacgctaccg atttcttggt attcgtaccc ccatcgccat tgcctggttg gtgacaggcc
    12001 gatgcaccgc ctgccgtagc tattgtcgtt ggaaaagcga gcgcgacacg aacgaactgg
    12061 gtacgaccgc cgccctggaa ctcatcgaag agatggccga agccggtgtg ctcatgatct
    12121 cgtttacggg cggcgagccg ttgctgcgtg acgatattgg gctgctgatt catcgggtca
    12181 aatccaaggg gatcgtttgc aagctcaata ccaacggtga tctcgtgttg cgccgtatcg
    12241 actcgttacg ctccctcgat ttgctgcaaa ttagtcttga tgggcccgct aagctgcacg
    12301 acccgttgcg gggaagagga agcgccgacc gggcttcgaa cgcggtacgg cgcgcgcatc
    12361 aagaaggcat tccggttcag gtcattacat gcctcacgcg ttacaatctc gatcggctgg
    12421 atgaggtgct tgcctacgca aaggccctgg atgttcgtct acacgtccaa ccccttgcct
    12481 gaacgcaaga aggcaaaaac tttcccacga cacgacagct agtcgatgcg ttggactctt
    12541 tgatcaggac gcgaaaacaa cgccatcatt tggcagatta tctagccacc acgtcgagtg
    12601 agcttcggca ctaccgggcg atgtactctc agggcttgcg aggttgccac tgcgccgtgg
    12661 ttaccgccac gctgctgccc gatggcagca tgatattttg tagcagcggg cgggagttca
    12721 ccgcttatga cacgaaaaca cttgggtttt ccggagcgtt tgcgaagctg aggccgccga
    12781 tttgcgatgg atgcacctgt gtgggaaagc ttcgcatatc caaggtatgc gacctcgacc
    12841 ccgccgtgat ttgggatctc gtcgtacaac gagggttgaa agcctcgcag caaggtaaaa
    12901 agggaagatg ggactgattg cgctcgatct gaatccgaca ccgaagcagg ttcgcatttt
    12961 tggaagcgtg ggaatccctt tgaccctggg cattcttggg ttggccggtg gaacacattt
    13021 cgagagttca tctaatgctc tatttcttgg ggtccttgcc gtgggggcga tcggctcgag
    13081 ctggctttat ccacccgtca tacgaattct gctccttgga tttcgcctat cgacctatcc
    13141 catcgcgctg gttttcgccc atattacgct cgccgctttt tttttcttgg tccttacgcc
    13201 cttgggtcta ctcgttcgat ggctccacgg cgatccgttg tcccgacgat gggatccttc
    13261 gagttcgagc tattggacgg cttgtacgtc atcggcaccg cgccgttatc ttcgtcctta
    13321 ttgaaccgtt tttttctgcg ccttgcgatc gacggcgcag cgagcatccg ccacaaacgc
    13381 tatgctgatc gagtcatgtc ggatcccaat aacgatttcg aggcgctttc ccggcaatcc
    13441 cgcggaggct ttttgtcgga cgtgtgggat ctgttgcgac acagcaagcg ctggtggctc
    13501 actcccatcg ttttcattct ggtccttttc ggcgccattc tggctcttgg cgcgtcgggc
    13561 gccgcgccat tcctctacac attgttttga gcatgggcct gcatcatcac cataagcgtc
    13621 acaaactgct cctagcgatg atcgcaggat tgatcaccct gctgggggtt gaagcaatca
    13681 gcaggtggtg gcttgaaggc aacgtgataa aaaccatcga aataccaacg gaaaagggac
    13741 caatccgacc agacgaccgc ttcggattcg ttcttgctcc tggcgattac gatggcacgc
    13801 acatcaatcg tatcggtctt cgaggtccgg agatcgagga acacaaacgc gcggaatgca
    13861 tccgcattct gatgcttggc ggttccacca cctatggcaa taccgtatcg accgaagaag
    13921 cctacccggc cgtcgtgcaa catctattgc ggcaaacgaa tccccaccgc tgcatcgaag
    13981 tcatcaatgc tggggtatct ggcgcttact cttatcatca cctggttcgc taccagcatc
    14041 tttacagcgc gcttcgtcca gacatcgtga cgatgtacat cggatggaac gactacgcgg
    14101 gttatctttg gcagcgagga gattggaaag ccgatagtct ggccgtcaac tcgctcatca
    14161 tcgatgtcgg ttcgataccg atggcgttgc tacggcgcag ttccgccgct cgaatcgcgt
    14221 atacgtccta caagcgggca agctttcgtc aggcgctatc cgaactatcc tcctgtacgg
    14281 atgtggcagc agctcttcgt ccggcaaccg aagggttgca tcacaacctg gcgcgtctca
    14341 tcgagctagt acaacgcgat ggcgcgaagg tgcttctgat caagtttcct tatgtgcttc
    14401 gggacgacca gatcaagcaa gacctggaac ttctttcgac gatggacgtg cctggcagga
    14461 tcgaatcgat gcttccgatg gtacgtttcg agcctagcat gccgacactg gcggcctcgg
    14521 tcatggatcg actcgcgcag gaaaaacgag tgggtgtggt ggattgtcgg accgtgtttt
    14581 acgatctatc gctagaagaa cgcctcgatc gattcgatga tgcgatacat ccgaacgcca
    14641 agggatacgc gttgatcggc aagtgtgt
//
LOCUS       DAOSOR010000064        14616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4154_length_14616_cov_4.134125, whole genome shotgun sequence.
ACCESSION   DAOSOR010000064 DAOSOR010000000
VERSION     DAOSOR010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 14616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14616
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4154_length_14616_cov_4.134125"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PK710_05320"
     CDS             complement(<1..532)
                     /locus_tag="PK710_05320"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOT09168.1"
                     /translation="MACMTIGCSSAFDDEESPEATQDEIEAWNSAYEENASGKADSAG
                     CSGVIVPDRNGFNKRIALTFDDGPSLANTQTVAEILEAHGTVGTFFVNGKNIKTQEHK
                     ALLKRLKAKGHIIANHTHTHVNMAQASAATVTSEIERTHSLLKELDLKPRFFRFPYGA
                     STCATADKVRSYGYAVT"
     gene            831..1439
                     /locus_tag="PK710_05325"
     CDS             831..1439
                     /locus_tag="PK710_05325"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOT09169.1"
                     /translation="MEYASFQAWLVDLDGTLYDPRGLKLVMAAYLAFHPGSLRLVHTF
                     RKQHERLREEGFESEDISAWEEQVARTAAILGESKERVGKLAYSWMVEIPCRWVRRFR
                     REHLMAEIAAFRAAGGKTALVSDYPATAKLRALEAEALFDVVIANGEPAGPGALKPSP
                     AGYLEASRRLGIAPAACLVVGDRDDADGEAARRAQMAFRLVR"
     gene            1767..2636
                     /locus_tag="PK710_05330"
     CDS             1767..2636
                     /locus_tag="PK710_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09170.1"
                     /translation="MGKYERKPFELLRLWGEVRKSSCFEPRSILHIRRVFAVVGGVTL
                     SLVLVPDARANPRPLPFTYPYATLSEDELEIEQYVDATPIRMLDSDGKKQWEPRYKLQ
                     TEFEYGLSDRFEFAFYLQYSQKPGGALGLDGTKQRVRFRLAEAGDWPVDVAFYLEVAE
                     LRHELEFEQKIILEKDIGQLQLITNLWFEQEIERYSERVYPILNPTFGASYALTPVFR
                     LGAEYWMHAKLGKDGFNADPHHFVGPAFAFIWGKLWWSTAAYARLDGMKRAVQPDDKY
                     GHVWIRSVVGLQL"
     gene            2633..2938
                     /locus_tag="PK710_05335"
     CDS             2633..2938
                     /locus_tag="PK710_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09171.1"
                     /translation="MNKVMTVLCMPFLLGLGLPALGCGTSTPPKTDAEKPERFAKIAA
                     IHKSKCGSCHTRIEPGSMPRDELEAELKRHRSRVKLSEAEWAEMLDYLAADSKSRER"
     gene            3020..3715
                     /locus_tag="PK710_05340"
     CDS             3020..3715
                     /locus_tag="PK710_05340"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HOT09172.1"
                     /translation="MSRALSLAFEIVFASSLMMMPACRPVSPSEPFHFEVGDILFQDL
                     DCGPLCDAIEAVTQGAHGASLSHVAIVSRVQDGDVWVIEAYAQGVVEVPVQQLLSRSH
                     DAQGQPKVLVGRLRPPLRHLISPAIQAARKRLGMPYDEAFMMNNGAYYCSELLYDIFV
                     EANAGQAVFELQPMTFRKPGSSYPHQVWQAYFEKRKEPVPEGKPGLNPGAMSRSSAIR
                     IVSALGAPAGWRR"
     gene            3826..4431
                     /locus_tag="PK710_05345"
     CDS             3826..4431
                     /locus_tag="PK710_05345"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT09173.1"
                     /translation="MSVDFWNKRYAENEPAYGTEPNDFLVSEVNRFVPAADVLCLGEG
                     EGRNAVFLAKRGMNVSALDYASAGLQRTRELARAQGVRVEPILADLTTFEPEENRYDG
                     VIAIFVHLPPKERIRMHHAALRALRPHGVFIAEYFSSDQLRFSSGGPKDPTMLVRIDD
                     LRQDFAMGRIERLEQQEVELREGRYHQGRASVVRIVVYRNL"
     gene            complement(4483..5916)
                     /locus_tag="PK710_05350"
     CDS             complement(4483..5916)
                     /locus_tag="PK710_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HOT09174.1"
                     /translation="MTTPGPDESETREALAAAAQWVATYLQESHRYPVLSTIRPGQIA
                     QSLAQHPPEHGERLEKLLRDVDTLIMPGITHWNHPGFFAYFGITGSAPGVMGEFIAAA
                     LNVNAMLWRTSPAATELEQRTLRWVAQMMGLPDTFFGEILDTASSSTFCALAAAREAA
                     GLDIRNRGMAGRSDLPPLAIYTSSEAHSSVDKAAIALGFGRDFTRRIEVDDAYRMRPD
                     RLSQAIESDLAKGIRPIAVVATTGTTSTTSVDPVRAIADICQQHDLWLHVDAAYGGSA
                     AIVPELRWVLEGCERADSLVTNPHKWMLTPIDCSLLYTRHPDALKRAFSLVADYLATD
                     DGDVTNLMDYGLALGRRFRSLKLWFVIRAYGRSGIAHIIERHVALANELATQIDDTPG
                     WERLAPTPLSTVCFRHVPPSLKDADAHNQAIVNKVNASGEVFVSSTKLRGSFAIRLAI
                     GNASTRRQHVQRAWELLQKAAAETSSP"
     gene            complement(5949..6386)
                     /locus_tag="PK710_05355"
     CDS             complement(5949..6386)
                     /locus_tag="PK710_05355"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOT09175.1"
                     /translation="MRLLIIAAGKIKERGLRDLIDDYLDRVGRHVRCQEVELRDTKDM
                     ESSFRSSIPPDAFTIALEVDGEALTSEQLSRLMVKRGSENKGIVAFLIGGADGLPPRL
                     SQQAHMRLSLSKMTLAHRVARVVLAEQLYRAVSIWKGEPYHRP"
     gene            complement(6399..6995)
                     /gene="rsfS"
                     /locus_tag="PK710_05360"
     CDS             complement(6399..6995)
                     /gene="rsfS"
                     /locus_tag="PK710_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOT09176.1"
                     /translation="MTIKDTKKHGASHDNPTEHSPRRNARPVLRVSHGTKQSDARPAP
                     PRVGVARTSTPPGPQDEPFRPPRRKRATAERHSSDTVHTALAIARAALEKKALNLEII
                     DLVGKADYADYLVLMSANSDRHVRAIAEEIVNELRKQKKRPLSVEGLNVGTWVLLDFG
                     DVVVHVFQSATRALYDIDGLWLDAARVPVSAEPTAEQS"
     gene            complement(7131..8717)
                     /locus_tag="PK710_05365"
     CDS             complement(7131..8717)
                     /locus_tag="PK710_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HOT09177.1"
                     /translation="MRYSYRMPRASRTALLLWITTTSVLAASWSFAQDGGAGAGGAGG
                     SGGGTSLPCDPNTLWCSNGAMAQIVESSDRLQGDIDTGWIPACKPATSTGHCKDEKIQ
                     FRAQLAFDKIKTGGPIYTIDMKKGTKVDARWPDTDWIDLTLPSGGATDGTFKVAHTLT
                     PEIALYIDTPVYTGEIKMDASVLINYLPNAQFNYTALNSTKFRPWAFDLVTLNVKGSD
                     LNNSKLFGITFGQITDILGVNGIDDYVDGSFSFNATTDTDFKYQTTQVNISGATGPIT
                     YADGKTQVMSPDGDYLEVQGNTQGVLRYSGTIEFLPVININSIGGVGVSLNFPISVGM
                     KYKFGSGAIPVSFPNELVHIPLPNVFVPSTPRNFGTVSTGSKSETQKVTIENSGELGA
                     LLEFSSSSSQFKIVGANAMELAPDGDSYDLEIRFQPTKSGKQTGKIIVKSNDPDTPTQ
                     EFNVTGYGEGADLPDPDPDPEEGTGGKGGGSSSSGSVYDNSSDDGGCGCRVPSQAQGS
                     AAASLALLGLVAIGLRRRRR"
     gene            complement(8733..10250)
                     /locus_tag="PK710_05370"
     CDS             complement(8733..10250)
                     /locus_tag="PK710_05370"
                     /inference="COORDINATES: protein motif:HMM:NF027116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HOT09178.1"
                     /translation="MCPSIRVGVASLLTAFVVTVGSVAHAKDGGPAWPEAGTDSGPST
                     ITPGAILAYYCGDADMQCMMAPIEFEHVHDLAPWLPDWDTGWVPKNFEQLQVRFTLKI
                     PADTEVKMGGKFRATWPSPITMATPGDRFNGFLKIDYGLIVKAEGKIDVSVLGQHITW
                     QGPLPYVPEVDFHLLGQNQFDSWAFKPDTVTASAFTKQIRLFRINVLYLAKIPKEIAE
                     GGVELDVRGELQATYFTEKMVIDPTVAPITTAGGETLHNFVGGPYVEVDVHPEGRIDY
                     DGIIHLIPAFYIEVLGKDFKIPFYDYPLNITDLLNLNLASKIVFKKVRIHVPLPDIPA
                     FEQPAIMDFGKVPVGSGEKLAIVIPNYGEAKARAVGEVEASKANIFKMLSSATMINPG
                     QSGEMVVRFTPKKIGKYEAKLTVRSNDPDLPEQTVMLKGQALDPNDPDLPEKDAGKDT
                     KIPEASGSSEDVQDDGGCGCRLAPHQTSTSWLGLLGLTVLGVARRRSRVQKMQRV"
     gene            complement(10426..12228)
                     /locus_tag="PK710_05375"
     CDS             complement(10426..12228)
                     /locus_tag="PK710_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09179.1"
                     /translation="MVRIALTTSLAALSLGIAAAPALADQPQSVVVIVDDDDDDGNFE
                     PDLLQNKAIPPPDLVELPNLSAFSGKPIPSTDAVRFVHQGKPLAVGTPLPSRDVRLQA
                     LVPGQHVVTFASHPLHVRAIRIMALDGASKPVSFTSSHASFQRTPPDRIDDVTRPHGD
                     PDALRFVLVGMKRDLPEHVRIDSRAQDGTLVDTLQRAKLVDVPCPPGTARELHCRSTW
                     PVRVVSDPMDQSHPLVVDRSVRAVLGGGLVVRVGDLAQQIRVGGPRSTRYGPIQRLKG
                     NLRITVPRTWPGGVPVLDSDSAAAMDMAREQFAGASAIWAQCGVTFGEPGPDTIRLLD
                     PPPPFLLAIGCGLGLPASGGVVRFSIEDQSLQVPVPAGFSPEQAARRIAREIEAMGFR
                     VTRSSNARIGPGALPEVDLMVFRQDGTPAHLASVQGEPLTTDPTLAVCVGRVDFAQGL
                     RHFLDLDSMTGTVEERTLLKWFDDRDPRTLDAVFIPAFGRGGGRIGESFIGTDRSTLR
                     NMVLVDRVGLSASNASHTLAHELGHVLLDVPGHPDDYGLDTPTLLMDSDAANASSFGP
                     RRLRIEDCERMWQQSGPRAPVALLRPWPFQPLSK"
     gene            complement(12216..13424)
                     /locus_tag="PK710_05380"
     CDS             complement(12216..13424)
                     /locus_tag="PK710_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09180.1"
                     /translation="MSIRATGELLNHALVDRLTADMASAVRRRPAHEVRLAGVLRALT
                     QYSTALRGAVAAGMETLVRRGSYDRSLYMAGIRALGEIDDERAGTLLSKALLSSEEAG
                     GMATLCAASFSRHPLAADPLAKVATSRHPHLAFLAEVARAARGECDGTHMTAIAPKIK
                     ESHRITMCERVFVPLSRFDRLPATVAPALAILRDAERHLGRWLVLAQIATRCSDPAPL
                     AEARDRSKAGPSSARAAWSFVAWALSGETEPPTARPTAELIARLSDRPTADRDSAFLF
                     RLAAVRAPTCRSMLENTAKSAPLEEEGAIRAACFLVKDYDRKDLRDALIVTAKTAKRE
                     DLRGLAMAALWDAGDKSTSFALAKPVSRSRSLPTAAWAGLIQAAHEHQWDHQLMDEPT
                     FRRLQWGWLE"
     gene            complement(13575..14345)
                     /gene="tatC"
                     /locus_tag="PK710_05385"
     CDS             complement(13575..14345)
                     /gene="tatC"
                     /locus_tag="PK710_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOT09181.1"
                     /translation="MTENSGEKTMTFWEHLDELRVRVRRALIAFILTSVAAWYAREYV
                     LAVLVYPFQKAWLQQNLPGSPQLHFAAPSDAFVAYVKQSMIAGAVFAAPFIFWQLWAF
                     IAPGLYAKEKKTALLFVVFSTLLFAMGGVFGWTVAFPTAFGYLLSLSGSVGADSVAIT
                     PTVMMTDYLDFVGRLLLAFGIIFEIPLVSLFLSMSGIINYKQMWKFGRWFVLIAFTLG
                     AVLTPPDVTSQLVMAIPMCLLYLVSIGLAYVFGRKPAA"
     gene            complement(14342..14539)
                     /locus_tag="PK710_05390"
     CDS             complement(14342..14539)
                     /locus_tag="PK710_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09182.1"
                     /translation="MAGVDAYGLGMDDLDPYRMLDEKRGLDEAEPAGPQSLDSSKDSS
                     HDSFSDDNIDVKRADSDQAKA"
BASE COUNT         3165 a         3958 c         4346 g         3147 t
ORIGIN      
        1 ctgtaacggc gtagccatac gaccggacct tatccgccgt ggcacacgtc gaggccccat
       61 acggaaaccg gaaaaaccga ggcttgagat ccaattcttt caataggctg tgcgtgcgct
      121 cgatctccga ggtcaccgtc gccgcagaag cctgagccat gttgacatgc gtatgcgtat
      181 gattggcgat gatatgcccc ttggccttca agcgcttgag caaggcttta tgctcttgcg
      241 ttttgatgtt cttaccattg acgaaaaacg ttcccaccgt accgtgagct tccaaaatct
      301 ccgccacagt ctgcgtgttc gccaaggaag gcccatcgtc gaacgtaaga gcgatgcgtt
      361 tgttgaaacc attgcggtca ggcacgatga ctcccgagca cccagcacta tcggctttgc
      421 ccgaagcgtt ttcctcataa gcgctgttcc acgcctcaat ttcgtcttgg gtggcctccg
      481 gggactcctc gtcgtcgaat gccgatgaac aaccgatggt catacaagcc attccaaatg
      541 ctgcacacag ggggaaccac agggtagatc gcatattgtt tttctccgag aggtgcgagc
      601 gctccctgcg aaccctgtgc caatttctga cattgtccta catgagccta tatttccttc
      661 ggtgaaggtt gtccttttcg aaatccttgc cgaaggcgcc agcgactgag aacggatcga
      721 tcccgctgga gcgggaatgg atcgtgccca gtgggtatcg ttcggcgacg ggacaagggc
      781 cgttttattt ccaggtgtcc gccaagacaa tctgctagcg aacgggtgtc atggaatatg
      841 cctctttcca ggcctggttg gtcgatctcg acggcacctt gtacgatccg cgggggctca
      901 agctcgtgat ggccgcctat ttggcatttc atcccggttc gctgcggttg gtccatacgt
      961 ttcgaaaaca gcatgagcgc cttcgtgaag aaggattcga aagcgaggat atctcggcct
     1021 gggaggaaca ggtggcgcga acggccgcca ttcttggaga atccaaggag cgtgtgggca
     1081 agcttgctta ttcgtggatg gtcgagatcc cttgtcgatg ggttcgtcgc tttcgccgag
     1141 agcatttgat ggcggaaatt gccgcttttc gagcggcagg gggaaagacg gctctggtga
     1201 gtgattatcc agccacagcc aagcttcgtg cgctcgaggc ggaggcattg ttcgatgtcg
     1261 tgattgcgaa tggcgaaccc gcagggcccg gtgcgttgaa accatctccg gcgggttatc
     1321 tcgaggcatc gcgacgactc ggtatcgccc cggcagcgtg tttagtcgta ggggataggg
     1381 atgatgcgga cggggaagcg gcgcgtcggg cccagatggc atttcgtctt gtgcggtgaa
     1441 acgctcggat ttattcggaa accaggatta ttaataaaat attacagaga cgtcagtgac
     1501 tgcggcttcg attgtgattc cttgtcgagg tcaagaagaa cgatttcgcg gactgtcacg
     1561 aagctggagt atcaaaggca attcgacgat tccgctcggg tgatttttct ccctgcctcg
     1621 agaggttgac gtcgcacctt tcggcaccag aggcgcgaca agccgttgtg aagcggtctt
     1681 gcgcaaactg ggctgttcac agctttggta ggcgttcggc gtatcgccat gcatggggat
     1741 gggtcgtcgg catggagaaa acagacatgg gaaaatacga acgaaagcct tttgaattgc
     1801 tgcggttatg gggagaagtc aggaaaagtt catgcttcga accccgctcc atcctgcata
     1861 ttcgacgagt ttttgcggtt gtggggggcg tgaccttgtc gctggtgctg gtgcctgacg
     1921 cgcgggccaa tccgagaccg ctgccgttta cgtatccgta cgcaacgcta tcggaggacg
     1981 aattggagat cgaacaatac gtggatgcga ctccgattcg catgttggac agcgatggca
     2041 aaaagcaatg ggaaccgaga tacaagcttc agacggaatt tgaatacggt ctgagcgacc
     2101 ggttcgagtt cgcgttttac cttcaatact cgcaaaaacc tgggggagcg ctcggtctgg
     2161 atggcaccaa gcagcgggtg cgcttccgtc tggcggaagc tggggattgg ccggtggacg
     2221 tcgcttttta cctcgaagtc gctgaactac ggcatgagtt ggagttcgag caaaaaatta
     2281 ttctcgaaaa ggatatcggc caactccagc tcatcacgaa tctatggttc gaacaggaga
     2341 tagagcgcta ttcagaacga gtctatccca tcctgaaccc gacgttcgga gcgagctatg
     2401 cgttgactcc cgtgtttcga ttgggagcgg agtattggat gcatgccaag ctcggcaaag
     2461 acggcttcaa cgccgatccg caccatttcg tcgggcccgc cttcgccttc atctggggaa
     2521 aattgtggtg gtccacggcg gcctacgcgc gactcgatgg gatgaagcgt gcggtgcagc
     2581 ccgatgataa gtatggtcac gtgtggatac gcagtgtcgt agggttgcag ctatgaacaa
     2641 ggtcatgacc gtcttgtgca tgccgttttt gcttgggctg ggattgccag cgcttgggtg
     2701 cggaacctcg actccaccga aaaccgatgc cgagaaaccg gaacgattcg ccaaaatcgc
     2761 cgccatccac aagtccaaat gcggatcgtg tcatacgcgc atcgagcccg gttcgatgcc
     2821 tcgcgacgag ctcgaagccg aactgaagcg ccatcgttcg cgcgtcaaac tctccgaggc
     2881 cgagtgggcc gagatgctcg actatttggc cgccgactcg aaatcgcggg agcgatgaac
     2941 cgtgccgtgg gtaacgaaga cgggcgctcg aagctgcttt cagcccgcga tcgggaatgg
     3001 gtcgtcgaaa cggcagtgga tgtctcgcgc tttatcgttg gcgtttgaga tcgtctttgc
     3061 ctcgagcctg atgatgatgc ctgcctgtcg ccctgtttcc ccttcggagc cgtttcactt
     3121 cgaagtgggt gatatcttgt ttcaagacct cgattgcggc cccctttgcg atgccatcga
     3181 agccgtcacg cagggcgctc acggagcatc gctatcccac gtggccatcg tctcgcgcgt
     3241 gcaagacggt gatgtatggg tgatcgaggc ttatgcacaa ggggtcgtgg aagtccccgt
     3301 gcagcaattg ctgtccagga gtcatgatgc gcaagggcaa cccaaggtgt tggtgggccg
     3361 ccttcgtcct ccccttcgtc atttgatttc ccccgccatc caagctgcgc gaaagcgtct
     3421 tggtatgccc tacgatgaag catttatgat gaacaatggg gcgtattatt gctccgaact
     3481 gctgtacgac atcttcgtgg aagccaacgc ggggcaagcc gtgttcgagc ttcaaccgat
     3541 gacgtttcga aaacccggtt cgagctatcc gcatcaggta tggcaggcgt atttcgaaaa
     3601 acgaaaagag ccggttccgg aaggaaaacc agggttgaac ccaggggcga tgtctcgctc
     3661 gagcgccatt cgtatcgttt ccgccctcgg agcgcccgcg ggatggcgtc gttgaacgct
     3721 cgtcgcacct ttgtctatcg atcatcgtct gtgttggaga ggccgctggt tggcaacctc
     3781 gagcatccgt tcggcatcgg agggccaaag gagcgccgac catcgatgag cgtcgatttc
     3841 tggaacaagc gatacgctga aaatgagccc gcgtatggga ccgaaccgaa tgactttctc
     3901 gtttccgagg taaaccgttt cgttcccgcg gccgacgtgc tgtgtctggg ggaaggggag
     3961 gggcgcaatg ccgtgtttct cgccaaacga ggaatgaacg tcagcgcact cgattatgcg
     4021 agcgctggtc tgcagcgcac ccgagaattg gcacgggctc aaggcgtgcg tgtcgaaccc
     4081 atcctcgcgg acctgacaac gttcgagccc gaagaaaacc gatacgatgg tgtcatcgcc
     4141 atcttcgttc acttgccccc gaaagaacga atccggatgc atcacgcggc gctgcgcgcg
     4201 ctacgtcccc acggtgtgtt catcgctgaa tacttttcaa gcgatcaact ccgcttttct
     4261 tcggggggac ccaaggaccc taccatgctg gtgcggatcg acgatttgcg acaagacttc
     4321 gcgatgggtc gcatcgaacg attggaacaa caggaagtgg agttgcgcga ggggcgctat
     4381 catcaaggtc gcgcttcggt ggttcgtatc gtggtgtatc gcaacctgtg ataacccgag
     4441 ggaatagaat aataaaaaca acaggttatg taatggtcgc ggtcatggag aggacgtttc
     4501 ggccgcggcc ttttgtagca attcccaagc tcgctgcacg tgttggcgcc gcgtggaggc
     4561 gttgccgatg gccagtcgaa tggcgaaaga acctcgcagt ttggtcgagg atacgaacac
     4621 ctcccccgag gcattgacct tgttcacgat cgcctggttg tgcgcgtccg cgtctttcag
     4681 cgatggggga acatggcgaa aacacacggt agaaagtggg gtaggggcca gtcgttccca
     4741 gccgggagta tcgtcgattt gcgttgcgag ttcattcgcc aacgccacgt gacgctcgat
     4801 gatgtgggcg attcccgatc gaccataagc ccgaatgacg aaccagagct tcagcgatcg
     4861 aaaacgacgt cccaatgcca gaccatagtc catcaggttg gtgacatctc cgtcgtcggt
     4921 ggcaagataa tcagcgacca gcgaaaaggc ccgtttcaag gcatcggggt ggcgtgtgta
     4981 caacagactg caatcgatgg gcgtgagcat ccacttatgc ggattggtca ccagcgagtc
     5041 ggcgcgctcg caaccctcga ggacccagcg caattcgggg acgatggcgg cgcttccgcc
     5101 ataagcggca tcgacgtgta gccaaaggtc gtgttgttga cagatatctg cgatggctcg
     5161 gacggggtcc acgcttgtcg tggatgtcgt gcctgtcgtg gccaccacgg caatcggacg
     5221 gatacccttg gccaggtcgg attcgatggc ctgggataga cggtccggcc gcatacgata
     5281 ggcgtcatcc acttcgatac gccgggtgaa atcccggcca aaaccgaggg caatggcggc
     5341 tttgtcgacg gagctatggg cttcggagga ggtgtagatc gccaggggag gcaggtcgga
     5401 acggccggcc atcccgcggt tacgaatatc gagtcccgcg gcctcgcgtg cggcggcaag
     5461 agcgcagaac gtggagctgg aagccgtgtc gaggatctcg ccaaaaaagg tgtcagggag
     5521 gcccatcatt tgcgcgaccc aacgaagggt gcgttgttcg agttccgtgg cagcgggtga
     5581 ggttcgccac aacatggcat tgacgttgag cgctgcggca atgaattctc ccatcacgcc
     5641 aggtgcggaa ccggtgatgc cgaagtaggc gaagaatcct ggatgattcc aatgcgtgat
     5701 gccgggcatg atgagcgtat cgacatcgcg cagcagcttt tcgagtcgct cgccatgctc
     5761 tggtggatgt tgcgcgaggc tctgagcgat ctgcccggga cgaatcgtgg aaaggacggg
     5821 atagcgatgg gattcttgaa ggtaggtggc gacccattgg gctgccgccg ccaacgcttc
     5881 tcgggtttcg gattcatcag gccctggggt tgtcatgaga ttcctcgtcg atgatgattc
     5941 gtgtggggtc atgggcggtg gtagggttcg cctttccaga tggataccgc tcgataaagc
     6001 tgttctgcaa gcacgacccg agccacgcgg tgggcaagcg tcatcttcga caggctcaaa
     6061 cgcatatggg cctgttggga caagcgaggg ggaagcccat cggctccacc gatcaagaac
     6121 gccacgattc ctttgttttc gcttccgcgc ttgaccatca gacgagacaa ctgctcactg
     6181 gtgagcgctt cgccatcgac ttccaaggcg atggtgaacg catccggagg aatcgacgat
     6241 cggaaggagg attccatgtc tttggtgtcg cgcagttcta cttcttgaca tcgaacgtgg
     6301 cgtccgactc gatcgaggta atcatcaatg aggtcgcgaa gtccgcgttc tttgattttt
     6361 ccagccgcga tgatgagcaa tcgcacgatc tatccggatc aggattgttc cgcggttggc
     6421 tccgcggaaa cagggacgcg agcggcatcg agccaaaggc cgtcgatgtc gtatagtgcg
     6481 cgggttgcgc tttgaaatac gtgaacgacc acatcaccga aatcgagaag aacccaggtc
     6541 cccacattca atccttctac ggatagcggt cgtttttttt gttttcgcag ttcgttcacg
     6601 atttcttcgg cgatggcgcg cacatgccga tcggagttgg cggacatgag aacgaggtaa
     6661 tcggcgtaat cggcttttcc aacgagatcg atgatttcga gattgagggc ttttttctcc
     6721 agcgcggcac gagcaatggc aagggctgtg tgaacggtat ccgaggaatg gcgttcggcg
     6781 gtggctcgtt tacgacgggg aggccgaaaa ggttcgtctt gtggacctgg tggtgtcgaa
     6841 gtcctggcaa cgccaactcg tggtggagcc ggacgagcat cggattgttt cgtgccatga
     6901 gagacgcgca gcaccggtcg agcgtttcgt cgggggctgt gttccgtggg gttgtcgtga
     6961 gacgcgccgt gttttttcgt atccttgata gtcaagcatc ctcccatgcc aatgctggta
     7021 ttgggttgaa agcgatcatg atgggatgaa acagtacggc agaacccgtg cccgcgaaac
     7081 cttatcaagg aatcgtggcg ttcgcatctg cggagaaagg ccgcgaaggg ttatcgtcga
     7141 cgcctccgaa ggccaatggc gacaagacca aggagcgcca aggaggccgc ggcggagcct
     7201 tgtgcttgcg acgggacgcg acatccgcat ccgccgtcgt ccgaagaatt gtcgtatacg
     7261 gagcctgagg aggaggagcc gccgcctttg cctccggtgc cttcctctgg atctggatcc
     7321 ggatcgggca aatccgcgcc ttcaccatag ccagtgacgt tgaactcttg ggttggtgta
     7381 tccggatcgt tgctttttac gatgattttt ccggtttgtt tgccggactt ggtgggttga
     7441 aaacggattt cgagatcgta gctatcccca tcgggggcca gttccatggc gttggcgccg
     7501 acgattttga attgggaact gctgctggaa aactcgagca aagctcctaa ttcgccggag
     7561 ttttcgatgg tcaccttctg ggtctcggat ttggaccccg tcgatacggt tccgaagttt
     7621 ctgggggtcg agggaacgaa aacgttcggc agggggatat gaacgagttc gttggggaag
     7681 gagacgggga tggcgccgga gccgaattta tacttcattc cgacactgat cgggaagttc
     7741 aggctcacgc caacaccacc aatcgaattg atgttgatca cgggcagaaa ttcgatggtg
     7801 cccgagtatc gcaagacgcc ttgtgtgttg ccttgcacct cgaggtagtc cccatcggga
     7861 ctcatgacct gcgtttttcc gtcggcgtag gtgatcgggc cggtagctcc gctgatgttg
     7921 acttgcgtgg tttggtattt gaagtccgtg tccgtagtgg cattgaaact gaacgaacca
     7981 tcgacgtaat catcgatacc gttgacgccg agaatatcgg tgatttgccc aaaggtaatg
     8041 ccaaagagct tcgagttatt gagatcgctt cctttgacgt tgagcgttac caagtcgaag
     8101 gcccatggcc ggaacttggt tgagttcaac gctgtgtagt tgaattgggc gtttggcaga
     8161 tagttgatga gcacggaggc atccatcttg atctcgccgg tatatacggg tgtatcgata
     8221 tacagtgcga tttcgggcgt cagggtatgc gctactttga acgtgccgtc ggttgctccc
     8281 cctgatggta acgtcaggtc gatccaatcg gtgtccggcc atcgagcatc gactttcgtc
     8341 cccttcttca tgtcgatggt gtaaatgggc ccgccagttt tgattttgtc gaaggcaagc
     8401 tgggcacgga attggatttt ttcgtctttg caatgcccgg tagaggtggc gggtttgcaa
     8461 gccggtatcc agccggtgtc gatatcccct tgaagtcggt cggaagactc gacgatttgt
     8521 gccatggcac cgttcgagca ccaaagggtg tttggatcac agggtaagct cgttccaccg
     8581 ccggagcccc ccgctccgcc ggctcccgct ccgccgtctt gtgcaaaaga ccaggaggcg
     8641 gcaagcacag acgtggtcgt aatccaaaga agcagtgcag tgcgtgaggc cctcggcatc
     8701 cgataagaat agcgcatgaa aaactttgcc tcctaaaccc tttgcatttt ctgaactcgg
     8761 cttcgccttc tggcaacacc aagcacggtc aatccaagca aacccaacca cgacgtcgac
     8821 gtttggtggg gcgcgagccg acatccgcag ccaccatcgt cttgtacatc ctcagacgaa
     8881 ccggaagcct ccggaatctt agtatcctta ccggcatctt tctcgggcaa gtcagggtca
     8941 ttcgggtcga gcgcttgccc tttgagcatc acagtctgct ccggcaggtc cggatcattc
     9001 gagcgcaccg taagcttggc ttcatatttc ccaatctttt tgggcgtgaa ccggacgacc
     9061 atctccccgc tctgcccggg gttgatcatc gtcgcggacg acagcatctt gaatatattg
     9121 gctttggatg cttccacctc gccgacggct cgtgctttgg cttctccgta attcggaatc
     9181 acgatggcaa gcttttctcc ggaacccacg gggaccttac caaagtccat gatcgcaggc
     9241 tgttcgaagg caggaatgtc gggaagcggt acatggatcc ggaccttttt gaaaacgatc
     9301 ttgctcgcca aattcagatt gagcaagtcc gtgatattca atgggtaatc gtaaaacgga
     9361 atcttgaaat ccttgcccaa tacctcgatg taaaaggctg ggatgagatg gatgatgcca
     9421 tcgtagtcga tccgtccttc gggatggaca tccacttcaa cgtaaggacc acccacgaag
     9481 ttatggagcg tctcacctcc tgccgtggtg atgggggcta ccgtgggatc aatcaccatt
     9541 ttttctgtga aatacgtggc ctgaagctct cctcgcacgt cgagttccac gccgccttcg
     9601 gcaatctctt tcggaatttt ggcgaggtac aatacgttga ttctaaagag tcgaatctgt
     9661 ttggtgaacg cgctcgcggt gaccgtgtct ggtttgaatg cccaggaatc gaattgattc
     9721 tgaccgagca agtgaaaatc gacctcaggc acatacggta aggggccttg ccacgtgatg
     9781 tgttggccca aaaccgacac gtcgatcttg ccctcggctt tcacgataag gccataatca
     9841 atcttcaaaa agccgttgaa acggtctcct ggcgtggcca tggtgatggg cgaaggccac
     9901 gtagcgcgga actttccgcc catcttgact tccgtatcgg ctgggatttt cagcgtgaaa
     9961 cgaacctgaa gctgttcgaa gttcttggga acccaccctg tatcccagtc cggaagccac
    10021 ggtgccaagt catggacatg ctcgaactcg atgggagcca tcatgcattg catgtccgca
    10081 tcgccgcaat aataggcgag aatggctcca ggagtgatgg tggatggacc ggaatccgtg
    10141 ccggcctcgg gccacgcagg cccgccatct ttcgcatgag ccacggatcc gacagtcacg
    10201 acgaatgcgg tcaacaggct cgcaacacca acacggatcg atggacacat attgcaagtg
    10261 tagcggggaa caccggccct aggcaaccaa cgattcgaat catagcaacg aagctccacc
    10321 aggctcaccc cgcagcggcg atgaacgtgt ggcctttccc gcaaaatcct tgggagattg
    10381 gcaagagcgt gtgtctggaa tcggctgcta tttgactaca ttcgcttatt tggaaagcgg
    10441 ttgaaaaggc caaggacgca acagagccac cggtgctcgg gggcccgatt gctgccacat
    10501 acgctcgcaa tcttcgattc tcaatcgccg tgggccaaaa gaagaagcat tggccgcatc
    10561 cgagtccatc aacaacgtcg gcgtgtccaa cccgtaatcg tcgggatggc cgggaacgtc
    10621 taacaaaaca tggcctagtt cgtgtgcgag cgtgtgagag gcgttcgagg cactcagtcc
    10681 gacccggtcc accaacacca tgttgcgcag cgtggaacgg tccgtgccga tgaacgattc
    10741 gccgattcga cctccccccc tgccgaaagc cgggatgaag accgcgtcga gcgttcgcgg
    10801 atcccggtca tcgaaccact tcaataaggt tcgttcctcc accgtacccg tcatggaatc
    10861 caggtcgaga aagtggcgaa gcccctgggc aaagtccacg cgccccacac aaacggccag
    10921 cgttgggtcc gtcgtcaaag gctccccttg cacactcgcg agatgggccg gcgtgccatc
    10981 ctgtcggaaa accataagat cgacttctgg caaagcaccg gggccaattc tggcattgga
    11041 agagcgagtg acgcgaaagc ccatggcttc gatttcacgg gcgatgcgcc gcgccgcttg
    11101 ttcgggggaa aatcctgctg gcacaggcac ttgcagcgat tggtcctcga tcgaaaaacg
    11161 cacgactccg cccgaagcgg gaagtccgag cccacagccg atcgcgagta gaaaaggagg
    11221 aggcgggtcg agcaatcgga tcgtgtccgg ccccggctcc ccgaaggtaa cgccacactg
    11281 cgcccagatt gcactggcac ccgcaaattg ctcccgcgcc atgtccatgg cggccgccga
    11341 gtccgaatcc aacacgggta cgcccccggg ccaggtgcgc ggaacggtga tgcgaaggtt
    11401 tcctttgagc cgttggatgg gaccataacg agtggaacgg ggccctccca cgcgaatctg
    11461 ctgcgcgaga tcccccaccc gaacgacaag acctcctcca agaaccgccc ggaccgaacg
    11521 atccacaacc aacggatgtg attggtccat gggatcagat actacccgca ccggccatgt
    11581 acttcggcaa tgcaactccc gcgctgtgcc cggtggacag ggaacatcta cgagctttgc
    11641 tcgttgaagc gtgtccacga gcgtgccgtc ttgcgctcgt gaatcaatcc tcacatgctc
    11701 aggcagatct cgtttcatcc ctaccagcac aaaacgcaag gcatcaggat ccccatgggg
    11761 acgcgttacg tcgtcgatgc gatccggagg cgtcctttga aacgaggcat gggaggaggt
    11821 gaaacttacg ggtttcgaag caccatcgag agccatgatt cgaatggctc gaacgtgtag
    11881 cggatgggag gcaaacgtga ccacgtgctg accgggaaca agagcctgca acctcacatc
    11941 acgggaaggt aaaggggtac ctaccgccaa cggctttccc tgatgaacga aacgaacggc
    12001 atcggttgat ggaattggtt tgccgctgaa ggcggatagg ttgggcaatt cgaccaggtc
    12061 tgggggagga atagccttgt tttgcaggag atctggctca aagttgccgt catcgtcatc
    12121 gtcgtcgacg atgactacga cagattgcgg ttggtcggcc aaagcgggag cagcggcgat
    12181 gccaagagat aaggcggcca acgaggttgt aagagctatt ctaaccatcc ccattgcaat
    12241 cgacgaaagg tgggttcgtc catgagttgg tgatcccact gatgctcgtg agcggcttgg
    12301 atgagtccgg cccaggccgc ggtggggaga gagcgtgaac gagagacggg tttcgcaaga
    12361 gcgaacgatg tagatttgtc cccggcatcc caaagggccg ccatggccaa cccgcgaaga
    12421 tcttcgcgtt ttgcggtttt ggcagtgacg atgagagcgt cgcgcaaatc cttgcggtca
    12481 tagtctttga caagaaagca cgcggcgcga atggcccctt cctcttctaa gggtgcggat
    12541 ttcgcagtgt tttcaagcat actgcggcag gtgggggccc gtacggccgc gaggcggaac
    12601 agaaaagcgg agtctcgatc cgcggtggga cggtcactga gtctggcaat gagttcggcc
    12661 gtggggcgcg ccgtgggagg ttcggtctct cccgacaatg cccacgccac gaaggaccaa
    12721 gcggctctcg cactggaagg accggccttg cttcggtccc gagcttccgc caatggcgcg
    12781 ggatcggaac agcgagtggc aatttgtgca agaaccaacc aacggccaag atgccgttcg
    12841 gcatcgcgta gtatcgcgag ggcaggggca accgtcgcgg gcaaacggtc gaatcgcgaa
    12901 agcggaacga aaacccgttc gcacatggtt attcgatggc tttctttgat cttcggagcg
    12961 atggccgtca tgtgcgttcc atcgcattcg cctcgggcag cgcgggccac ttctgctaga
    13021 aatgcaagat ggggatgacg gctcgtggcc actttggcaa gggggtcggc cgccagtgga
    13081 tggcgtgaaa agcttgccgc gcaaagcgtc gccatgccac ccgcctcttc tgacgataag
    13141 agtgctttgc tgagcaaagt acccgcgcgt tcgtcgtcga tctctcccaa agcgcgaata
    13201 cctgccatat aaaggcttcg atcgtaggag ccgcgacgaa cgagcgtttc cattcccgct
    13261 gcgaccgcgc cacgaagcgc tgtgctgtat tgcgttagcg ctcgaaggac gcccgcgaga
    13321 cgcacctcat gggcgggtcg tcgtcgcacg gccgaagcca tgtccgctgt caggcggtcg
    13381 accaatgcgt ggttcagcaa ctcccctgtg gcgcgaatag acatgcgtat tctcaccacg
    13441 ataccagagt cttcgccatg ctggtggcca acgacctcag aaacgtgctt tatgcaaatt
    13501 cttgcaataa cgacttgaca gacacgagcg gtaggcacgg ggtgtctgac atggcgagcc
    13561 gacggctgga cacatcaagc ggcgggcttt cgaccgaaca cataggcgag tccaatcgac
    13621 accaggtaca acaagcacat gggaatcgcc atgacgagct ggctcgtgac atcgggaggc
    13681 gtgagtacgg ctccgagcgt gaaggcaatc aatacgaacc agcgcccgaa cttccacatt
    13741 tgtttgtaat tgatgatgcc ggacatcgat aaaaacaggg atacgagtgg tatttcaaag
    13801 atgattccga acgccaatag caatcgaccc acgaagtcca agtaatcggt catcatcacg
    13861 gtgggtgtga tcgcgacgct atcggcgccc accgaaccag aaagactcag cagataaccg
    13921 aacgcggtag gaaacgccac ggtccaaccg aatacgccac ccatcgcaaa caatagcgtg
    13981 gaaaaaacca cgaatagcag cgccgttttc ttttctttcg cataaaggcc cggtgcaatg
    14041 aatgcccaca actgccaaaa aatgaagggc gccgcgaata ccgcccctgc aatcatcgat
    14101 tgcttgacat aagctacgaa tgcatcgctt ggtgctgcga aatgaagctg cggagagccc
    14161 ggtaagttct gttggagcca cgccttttga aaaggataaa ccaacaccgc cagcacgtat
    14221 tcgcgggcgt accatgccgc gaccgaagtc aaaataaagg cgatgagagc acgacggaca
    14281 cgcacacgga gttcgtccag gtgctcccaa aaggtcatcg ttttctcgcc actattttcc
    14341 gtcatgcttt cgcctgatcc gagtccgctc gtttcacgtc gatgttgtca tccgaaaacg
    14401 aatcgtgcga tgaatccttc gagctatcca aactctgtgg ccccgcgggt tccgcttcat
    14461 ccaaaccacg tttttcgtcg agcattcggt acggatccaa atcatccatt ccgaggccat
    14521 aagcgtccac ccctgccacg ggatactccc gatcccgcat gtccggtcgt cgggctcgcg
    14581 caacggcaac ctttcagaat ccgtttttga aggttt
//
LOCUS       DAOSOR010000065        14567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4164_length_14567_cov_4.663175, whole genome shotgun sequence.
ACCESSION   DAOSOR010000065 DAOSOR010000000
VERSION     DAOSOR010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 14567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14567
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4164_length_14567_cov_4.663175"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="PK710_05395"
     CDS             <1..675
                     /locus_tag="PK710_05395"
                     /inference="COORDINATES: protein motif:HMM:NF019840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol transfer protein"
                     /protein_id="HOT09183.1"
                     /translation="EDGENKEVHGVIDTGGRLFVNLGSEGISLDVGRHRIVMVVAGDL
                     TYAEQFIEVIRPQARVVVTDIDGTLTSSEYAALTDVAGLPPAEAHPGAADMLWAFAIR
                     GYKVFYLTARPEWMTPLTREWLPLRGFPPGVLHTTLSKIGAVGSSATDAKTKELAWLH
                     ATTTIVPSYAFGNKTSDVAAYGGAGIEPTGCYYYELDGDAKGGTIHADYTALVAVAEQ
                     APLACF"
     gene            complement(694..1341)
                     /locus_tag="PK710_05400"
     CDS             complement(694..1341)
                     /locus_tag="PK710_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09184.1"
                     /translation="MTRMFSALSIVAILVALPGCEERPAAQETPAVSKTPEPTATPPL
                     KASEPAPTASAPKPSHACPEGSSGEGTFKDPCEAKGKTRVMTAEWTGKMDDKGPSFRV
                     TNKTNLEILFGQIVVYFYDKSGKLIEVPGGSDSKEHKTKQKCSGNIFAGPMKPGEKAV
                     LTFSCVNKSHVPENTAHIEAELRMVGFTGSDGKKADTYWRNEELTPDDRPKGGIK"
     gene            1613..3157
                     /locus_tag="PK710_05405"
     CDS             1613..3157
                     /locus_tag="PK710_05405"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF006695.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOT09185.1"
                     /translation="MLHYPFGESYADEPVNQEIALHTLSARYRHSLTLLTDLYQLTMA
                     YGYWKNGLADRQAVFHLAFRKCPFGGGYAIAAGLHDVVGFLESMRFDESDISFLHTLH
                     GHDGKPLFEPEFLRFLGELTLECDLDAIPEGTAVFAHEPLIRVRGPLVQAQIIETALL
                     NIINFQTLIATKAARVCFAAKGKPVLEFGLRRAQGIDGALSASRAAYIGGCEATSNVL
                     AGKLFGIPVRGTHAHSWVMVFDDELEAFAAYARAMPNNCVFLVDTYDTLEGVRNAVQI
                     GHRLREQGHELSGVRLDSGDLAFLSIEARKILDEAGFPNAAIVASNDLDEQLIDSLQN
                     QGAAIGVWGVGTKLVTAFDQAALGGVYKLSAIQDACGQYQPKIKLSEQAVKISTPGIL
                     NVRRFRLEGQAIGDMVFDELDAIPDSPTIVDPADATRRKVFSPDMTREDLLEPVFRKG
                     RLVRESPPIDAIRMRVKSQLEALHAGVKRFLNPHSYPVGLEEKLFNRRRELVFEARGH
                     HIREVK"
     gene            3157..5169
                     /gene="nadE"
                     /locus_tag="PK710_05410"
     CDS             3157..5169
                     /gene="nadE"
                     /locus_tag="PK710_05410"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOT09186.1"
                     /translation="MQWIKVGAAILNQTPLAWDQNRDNILEAISCARDQGVSLLCLPE
                     MCIPSYGCQDAFQFPSVQRTCLNVLFEEIVPATKDIVTCVGLPLVFHNSLFNAVCLIA
                     DRKVVGFVCKRFLAGDGVHYEPRWFKPWKQGFRAHVEMHGQCYPVGDIYFNVGGIKIG
                     FEICEDAWVAARPGGDMAGRGVDVILNPSASHFAFDKLEVRKRFVSEGSRAFGVTYVY
                     ANLVGNDEGRIVYDGGALIASAGQLVAAGRRFGYRDVEMTTAIVDVDTTRLRQCWTNS
                     FMPRLEQNPNECASTPFSWPSLAPPAVSVPREEAWESSVHHYYEEFTRSIGLSLFDYM
                     RCSRSHGFVVSLSGGVDSTACATLITIMVHLAVADLGLEGFKQKLAYWPALQTARSVQ
                     DLVRQLLLCVYQSTRNSTLGSRQTAMAVAEALGCDFLQFDVDTLVQQVVAMVEGGIGR
                     HLTWEQDDISLQNIQARVRGPGAWMLANLRGALLVATSNRSETAVGYATMDGDTCGGI
                     SPMAGIDKGFLQGWLAWLESHGPESFGALPAIGVVNAQRPTAELRPAQYEQTDEDDLM
                     PYPILDAIQEAAIRDKRDPIEVFLLMHARFTEIDPRQLCVWVERFFVLWCRNQWKRER
                     YAPSFHVDDTNLDPKSWCRFPILSGGFSRELALLRAYVKDNFPDEA"
     gene            complement(5177..6010)
                     /locus_tag="PK710_05415"
     CDS             complement(5177..6010)
                     /locus_tag="PK710_05415"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT09187.1"
                     /translation="MESVPHVDDDRYQVEAKVGEGAIATVWKALDRVTGQLVAIKLMR
                     INRMLPETVQRLAQEVEVLRRLQHPNIVGVVGAGTAQDTGAPYIVLEWLDNVSLRFYL
                     QRRRQPHPSFILNIVSGVAHAMSAAHEIGVVHRDLKPENVLLCGPDRNHVKVVDFGMA
                     KLPMELARLVTLRSTLFGTPQYMAPERAKGQPVTPATDVYALGIITYEMFAGQRPFEA
                     DEASTLLRKHVYEPVPPLPSVSPAVETVLLEALSKNPMQRPSAQRFAIDLQKALCSSS
                     E"
     gene            6106..6435
                     /locus_tag="PK710_05420"
     CDS             6106..6435
                     /locus_tag="PK710_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09188.1"
                     /translation="MFHVRLDGRHRWMRVTPAFVVVASLLLACGGNKQEPAKTAADTK
                     QETVPGMPKSGDEGWCDPMPDLGESCKSYLKECTIVCDYMSDTCAVLVCMSGRWEYVE
                     RGEESQD"
     gene            complement(6442..7269)
                     /locus_tag="PK710_05425"
     CDS             complement(6442..7269)
                     /locus_tag="PK710_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOT09189.1"
                     /translation="MIDASRVGFVENAEEITDRPVWGAGFGARTTEELARCASSFYRL
                     AMILPGRLQSTTLGDVLGRLHRAQATGELELIGHDAPYRGCCHRIVMRDGMVCDVGTP
                     IPVPRLGEVLLEQGDLSIEQHHRFVDELQRLPKVKAGEILVREGFVTQQQVENGLGRQ
                     TRLRLDAVFSLQQADIRFHPLGRFHSKRKLEPFEFLHGRRRARGADSPALPLRQAYET
                     LGLSPNASRAQVRRTFRKLATTCHPDLFTNLPESQRRQVEARFSALSSAYHRIVTQD"
     gene            7330..9945
                     /locus_tag="PK710_05430"
     CDS             7330..9945
                     /locus_tag="PK710_05430"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT09190.1"
                     /translation="MPSAKKTSNVPIGTTLDGKYRITREIGRGGMASIYEAVNVDIGK
                     KVAVKVLNPELTNSAVVVERFLREARAAAAVKSPYICDVYDAGRLEDGRPFLVLELLE
                     GESLYERMVKVRRFEPDFVARVFSQVARGLTKAHEGNIIHRDLKPENIFITHNDDGEV
                     LAKIVDFGLAKFYAPIDGDAAQQRLTREGAVFGTPAYMSPEQVKGQGQVDLRADLWAV
                     GCMVYECLIGRTVWATEQGVAMIFAQIATAEIPVPSLIRRDLPITFDKWFAKALARDP
                     DQRFQSAKELADELVASLGQGPPSIILSQGIQDIEQAALQPPISSDHAFAPTERPPPP
                     GLEADPLDDNAPTRQMAVPDEYRAMPTDAAVNRSESAKPRTSYRTWIAIAGALLVAAI
                     GYVAWTQLLPAPAPAVVSSQPVTHPVVTASASQKTPEVLVPTRITKDLPDWAKNIASA
                     QELLSKGDIEGASETFRSAIDGNTSGIPRVMLENSRLAANSKGTCELGGLGRPRPFEL
                     TNSIRRPAIVFTSHGPLVTWADDHEVPGQWHAYTQLLDDALRALGNPVDVTPEAISVQ
                     TPRLIAMDNQVLLLYSDQHGKNPGVFVRRLTTTGRILGAPLQVASERTSVVFPSITKN
                     PWGGFWLAYVEDSDKSPSSKLFVQSLTHDVKLDGERKLMAEYALRYGLHRSRTNAPSV
                     AAGTNALLLSYRVEQGRDHRTVLQHIALEESKIPPGLSEVNPPTLDRVVGDVRDLSEG
                     KIRLYPPMMHCENSACFVVWREEPRGSNAAYIDPTTGTTIWRKRFAHRGMQVSVGLDA
                     AGNGLLVWYQDGRVHVSPISRDGIGVPSVVGRVTGEQPVPSVSRGKGHGEWLIAWIDY
                     EAGHLEAYLASVTCK"
     gene            complement(9946..10533)
                     /locus_tag="PK710_05435"
     CDS             complement(9946..10533)
                     /locus_tag="PK710_05435"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOT09191.1"
                     /translation="MISLPARSSARLAPIGSKRLLLFPIEGSDARELWCAVQESRKYL
                     EPWLPWVPFQVDESSMHRFAEACAMDWDSGRAIRFVIRERETRTLVGVVGLENCTPMH
                     ACCDLGYWLREPAQGRGYMTEAAGILIQEAFDKYHMHRIRVAAATTNYRSLSVIGRLG
                     FRFEGIARQAEYCAGRWLDHAQFGMLVTDHAARRP"
     gene            complement(11081..12634)
                     /locus_tag="PK710_05440"
     CDS             complement(11081..12634)
                     /locus_tag="PK710_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09192.1"
                     /translation="MAKRGHCARWVTLWAWGIGAGILSWGCSSSSESEGETTNPKVDA
                     AVGDAAISSDARQESGTDTGGNSDVFDEWPPDALPDAFSDIVSEVSSGCGNGVCDPGE
                     DYGNCAVDCGQCPPTCGNGVCDPGEDCGNCPQDCGSCSEADDLFKCIFDPMQWWKGAD
                     KRDITFVGFGDPHAADPSPGCSVNKNDAPNQNLLIRQAINSIGTHVWPKGASFAQEDK
                     PYSHVRGVIIAGDLTQTGSESVPAGQAVCRQYTVYREAFGRCGTENKLSFPVYDLYGN
                     HDFPRAALPGNPDHHPVIDYLDRITQAHRPGDAKDLYDDATPGTGHYAWRWDDIWFVN
                     VNVKPGWKAENLPNETNTNVRIVDPHDSRRFLKEFLLSRKNSAKRQIVITAHYGLGSL
                     DAEEKESFCKLIHHAQHGTGSFEGIGKKLSPNWPVLAYIHGHNHHRPEHKEFPCPSPY
                     QDIIIPTFSLGTPFYQGPHNVQGQLHFTIMRIGANKLEVVGVSAPANNPTGPWDYLYR
                     IRLGYPVQP"
     gene            13220..>14567
                     /locus_tag="PK710_05445"
     CDS             13220..>14567
                     /locus_tag="PK710_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT09193.1"
                     /translation="MRRFIALTLTLGFLVSGCGTSTEADSPPTTPNGTASCGDSVCDE
                     SESCETCPEDCGDCEDGPNFCGDFVCDDEEDCVSCPEDCGDCFSEITCGDDICSETES
                     CSTCPEDCGQCIESHCGDGQCSGIETCDTCPADCGPCKTSCGDGSCQEDENCLSCSAD
                     CGSCAPGHLDIWSPRPIAPQFAVAGGAIQAEVNGKDTWNPNEWYAVVRNTYRTWPCKV
                     TAKYGSIDYGRRKGWILDIQLPIYVPPELLELAIAHGEAGTGSRIRSVQMVASFDEDF
                     YIIHQTDHHIAAKNAKRPDGTVDLGGGPKPGTSQALTWGAKAINIINPRFVMLTGDNI
                     FVIFEDKNSWRGLKRSVEILNYFKEGINTYQVATLVTSGNHDVGTNIHTTPDQLKYLE
                     EYANILGPRSFSRRMGPFYIGLFEWSRSQFTEAMTKDYNASFQDPLVQFRLVGSHFN"
BASE COUNT         3192 a         4249 c         3867 g         3259 t
ORIGIN      
        1 gaagacggtg aaaacaaaga ggtccatggg gttatagaca ctggcgggcg actgttcgtg
       61 aaccttggta gcgaagggat ttctctcgat gtgggacgtc atcggatcgt gatggtcgtg
      121 gctggagatc tgacgtacgc tgaacaattc atcgaagtca tccgaccaca agcacgagta
      181 gtggtcacgg atatcgatgg aacgctgacg tcgagtgaat acgcggcgct cacggatgtc
      241 gccggattac ctcctgccga agcgcatccc ggagcggccg atatgctgtg ggccttcgcg
      301 attcggggat acaaggtgtt ttatttgacc gcacggcccg agtggatgac gcccttgacg
      361 cgagagtggt tgcccttacg cggatttcct ccgggcgtgc tgcatactac gctcagcaaa
      421 atcggagccg tggggtcctc cgcgacagat gccaaaacca aagaactcgc atggcttcac
      481 gccaccacga cgatcgtacc ttcttacgct ttcggtaaca aaaccagcga tgttgcggcg
      541 tacggggggg caggtatcga gccgacggga tgttattatt acgaactcga tggagatgcg
      601 aaaggcggaa cgatccacgc ggactacacc gcgctcgtcg ctgtggcgga gcaagctccc
      661 ctggcttgtt tctgatgttt ctgaaacggg gagctacttg atgccgccct tgggccgatc
      721 gtcaggtgtc agctcctcat tgcgccagta cgtatcggct ttcttgccgt cggatcccgt
      781 gaaaccaacc attcgcaatt cagcctcgat atgagcagtg ttttccggca cgtgcgattt
      841 attcacacag gaaaacgtga gcactgcctt ttctccgggc ttcatcggac ctgcaaaaat
      901 atttcccgaa cacttctgtt tggtcttgtg ttctttgctg tccgagcctc ccggcacttc
      961 gataagcttg ccggacttgt cgtagaaata aaccacgatt tgcccaaaca ggatttcgag
     1021 attcgtcttg ttggtgacac gaaatgaggg gcccttatca tccatttttc cggtccattc
     1081 cgccgtcatg acccgcgttt ttcccttggc ttcacagggg tccttgaaag tgccttcgcc
     1141 cgaagagcct tccgggcacg catgagacgg cttcggggcc gatgcggtag gagctggctc
     1201 actggctttg agggggggag tggcggtggg ttcgggcgtt ttgctgaccg ctggcgtttc
     1261 ctgggccgca ggacgttcct cgcatcccgg tagtgccacc aaaatagcaa cgatagacaa
     1321 ggctgaaaac attcgtgtca tggtcctcca ccgcgcccgt gctgcggctg agcgtggtgg
     1381 cttctcggta ctccaaggcg ttcgcggtga caacggtccg gttcatggcg cggttgttct
     1441 catcgatgcc cggtgatgag acgatggaag caaaaactcg gcattttcac ctacgtccgt
     1501 catggctccc agggaaatct caattcccat tgactcattt ttcggctttc gatttcgatg
     1561 attcatcctg ctcggctgcc gaaccccaca aatttatgct cgccatcact ccatgctcca
     1621 ttatcctttc ggcgaatcgt acgcggacga accggtcaac caggagatcg ccttgcacac
     1681 attgtcggct cgataccgtc actccttgac gctcctcacg gacttgtatc aactcaccat
     1741 ggcatacggc tattggaaga atgggcttgc cgaccgacag gctgtctttc atctggcgtt
     1801 tcggaagtgc ccgtttggtg gtggatacgc cattgccgca ggattgcacg atgtggtcgg
     1861 cttcctcgaa tcgatgcgtt tcgacgaaag cgatatctcg tttctacaca ccctgcacgg
     1921 tcatgacgga aagcctttgt tcgaaccgga atttcttcgc ttccttggcg agcttacgtt
     1981 ggaatgcgat cttgacgcta tccctgaagg gacagcggta ttcgcccacg agccattgat
     2041 tcgtgtgcga ggcccgctgg ttcaagcaca gatcatcgaa accgcgttgc tcaatatcat
     2101 caactttcag accttgattg caaccaaggc cgcacgtgta tgctttgccg caaaaggaaa
     2161 accagtgctg gaattcggat tgcgacgagc acaagggatc gatggggccc tgtccgcgag
     2221 tcgtgccgcg tatattggcg gttgtgaagc aacctccaat gtgctcgcgg gaaaattatt
     2281 cggcatcccc gttcgcggaa cgcatgctca tagctgggtg atggtattcg atgacgaact
     2341 cgaggcgttt gccgcgtacg cacgcgcgat gcccaacaac tgtgtctttc tcgtggatac
     2401 gtatgacacg ttggaaggcg tccgaaacgc ggtgcaaatc ggtcaccgct tgcgggaaca
     2461 aggccacgaa ctcagcggcg ttcgcctgga ttcgggagac ctggctttcc tcagcatcga
     2521 agctagaaaa atcctcgatg aagcgggctt ccccaacgcg gccatcgttg cttccaatga
     2581 cttggatgaa caactcattg atagcctgca aaaccaaggc gctgctattg gggtatgggg
     2641 tgtgggaacc aaactcgtca ccgccttcga ccaagcagct ttgggaggag tgtacaaact
     2701 atctgccatc caagacgctt gcggtcagta tcaaccaaaa atcaaactca gcgaacaagc
     2761 cgtaaaaata tccactccgg gtatcctcaa cgtgcgacgt ttccgtctcg agggtcaagc
     2821 catcggtgac atggtttttg atgaactcga tgccatcccg gattctccca cgatcgtgga
     2881 cccggcggat gccacgcgca gaaaagtgtt ttctcccgac atgacccgcg aggatttgct
     2941 cgagcccgtg tttcgaaaag gccgcctcgt tcgtgaatca ccgcccatcg acgcaatccg
     3001 aatgcgagtc aagtcccaac tcgaggcgtt acatgccggg gtcaaacgct ttctcaatcc
     3061 ccattcttat cccgtgggtc tcgaagaaaa attgttcaac cgtcggcgtg agcttgtgtt
     3121 cgaagcacgt ggtcatcaca ttcgcgaggt caagtgatgc agtggatcaa ggtcggagcg
     3181 gccatcctca accaaacgcc gctggcttgg gaccaaaatc gagataacat cctggaagcc
     3241 atatcctgcg ctcgcgacca gggcgtctcg ctgctttgcc tgcccgagat gtgcatccct
     3301 tcgtatggtt gtcaggacgc tttccaattc ccaagtgtcc aacgtacttg cctgaacgtt
     3361 ctgttcgaag aaattgtgcc agccacgaaa gacatcgtca cctgcgtggg acttcctctc
     3421 gtctttcaca atagcctttt caatgccgtt tgcctcatcg ccgaccggaa ggtcgttggt
     3481 ttcgtttgta aacgctttct cgcgggtgat ggcgtccact acgagcctcg ctggtttaaa
     3541 ccatggaaac aaggttttcg ggcgcatgtg gaaatgcacg gccagtgcta tcccgttggg
     3601 gatatctact tcaacgtcgg tgggatcaaa atcggttttg agatctgtga agatgcctgg
     3661 gttgcggctc gtccgggagg ggatatggct ggtcgtggcg tggacgtcat ccttaaccct
     3721 tcggccagcc atttcgcttt cgataaattg gaggtacgaa aacgtttcgt atccgaaggc
     3781 tccagagcct ttggtgttac ctacgtgtac gccaatctcg tgggaaatga tgagggaagg
     3841 atcgtgtacg acgggggggc tctcatcgca agcgccggtc aactggtcgc ggccggaaga
     3901 cgtttcggat atcgcgatgt cgaaatgacg accgcgatcg tcgatgtgga taccacgcgg
     3961 ctgcgacaat gttggaccaa cagcttcatg cctcgtttgg agcagaaccc caacgagtgt
     4021 gcgtcgacac ctttttcatg gccatcgctt gcgccacccg ccgtgagcgt gcctcgtgag
     4081 gaagcgtggg agtcgtccgt tcatcattat tatgaagaat ttacgcggtc cattggcctg
     4141 tctcttttcg actacatgcg ctgctcgcgt tcacacgggt tcgtggtttc cttgtccggt
     4201 ggcgtcgact ccaccgcttg cgcaactctt atcacgatca tggtccatct cgccgtagcg
     4261 gaccttggac tcgaagggtt caagcaaaag ctcgcatact ggcctgcgtt gcaaactgcg
     4321 cgatccgtgc aagacctcgt tcgtcaactg ctcctttgtg tctatcaaag tacacgcaac
     4381 agtaccctcg gttcacggca aacggcgatg gccgtcgcgg aagccttggg atgcgacttc
     4441 ctgcaattcg atgtcgacac attggtccag caggttgtgg cgatggtgga aggaggaatc
     4501 ggtcgccatc tcacgtggga acaggacgat atttccctgc aaaacatcca ggctcgtgtg
     4561 cggggaccgg gggcgtggat gctcgccaat ctgcgcgggg cattgctcgt ggcgacttcg
     4621 aatcgatccg aaacggccgt tggctatgcc acgatggatg gggatacctg cggaggtatc
     4681 agtccaatgg caggaatcga caaaggtttc ttgcaaggtt ggctcgcatg gctcgaaagc
     4741 cacggtccag aatcgttcgg cgcgcttccc gcgatcggcg tcgtcaatgc gcagcgtcct
     4801 accgctgagt tgcgccctgc gcaatatgag caaaccgatg aggatgactt gatgccgtat
     4861 cccattttgg atgcgattca agaagccgcg atccgggaca aacgcgatcc catcgaagtg
     4921 tttttgctca tgcatgcgcg gttcaccgaa atcgatccac ggcagctctg tgtttgggtg
     4981 gaacgtttct ttgtgctctg gtgtcgaaat caatggaaac gagagcgtta tgctccttcg
     5041 tttcacgtgg atgataccaa tctcgatcca aaaagctggt gccgctttcc catcttgtcc
     5101 ggaggttttt ctcgggagct tgccttgctt cgagcttatg tgaaggacaa ctttcccgac
     5161 gaagcatgag cctcgttcac tcactcgacg aacaaagagc cttctgcaaa tcgatggcga
     5221 agcgctgtgc cgaggggcgt tgcatgggat ttttcgatag ggcttcgagc aatacggttt
     5281 cgactgcggg ggaaacgctt ggcagcggtg gaacaggttc ataaacgtgt tttcgaagaa
     5341 gggtcgaggc ttcgtcagct tcgaaagggc gctgccctgc aaacatctcg tacgtgatga
     5401 tgccaagcgc atacacatcg gtggctggcg tcaccggctg tcccttggcc cgttctggtg
     5461 ccatatattg cggagtccca aacagcgtcg accgcaaggt gacgagccgt gcaagctcca
     5521 tcggaagctt ggccatgccg aaatccacga ctttgacgtg gtttctatct ggaccgcata
     5581 acaaaacatt ctcgggcttc aagtcgcgat gaacaacgcc aatctcatgc gctgccgaca
     5641 tcgcatgcgc cacgcccgac acgatattga ggatgaagga aggatggggt tgccgtcgac
     5701 gttgcaagta aaaacgcaaa gatacgttgt cgagccattc gagcacaatg taaggggcac
     5761 ctgtgtcttg ggcggttcct gctcccacca caccaacgat attcggatgt tgtagccgcc
     5821 gaagaacttc gacttcttgc gcgagacgct gcaccgtttc cggcaacata cggttgatgc
     5881 gcatgagttt gatcgccacg agttgccccg tcaccctgtc cagagctttc catacggtag
     5941 caatggctcc ctctccaact ttcgcctcga cttgatatcg atcgtcatca acgtggggga
     6001 cagattccat gcctgctcct gcctgacacg cttcgaggta ttgttcaagc aaagggtgtc
     6061 atcggatcag tggacgagaa cacgactcct cggtagcatc ggagcatgtt ccacgtccgc
     6121 cttgacgggc gccatcgttg gatgcgcgta acccctgcct tcgtggtggt agcgtcgttg
     6181 ctcttggctt gtggcggaaa taagcaggag cctgcgaaaa ctgcggcgga taccaaacaa
     6241 gaaaccgtgc ccggtatgcc aaaaagtggc gacgaaggat ggtgtgaccc catgccggac
     6301 cttggggagt catgcaaatc ctatctgaaa gaatgtacga tcgtctgtga ttacatgagc
     6361 gatacctgtg ctgtgctggt gtgcatgagt ggacgttggg aatacgtcga acggggagaa
     6421 gaatcccaag attgacgcgg attagtcttg cgtgacgatc cggtgatacg cgctggataa
     6481 agcgctgaat ctcgcttcca cctgtcgacg ttgggattct ggcaagttcg tgaaaagatc
     6541 gggatgacag gtcgtggcaa gcttccggaa tgtgcgacgt acttgcgctc ggcttgcgtt
     6601 tggcgatagt cccagggttt cataggcttg tcgtaacggc agggcaggcg aatcggctcc
     6661 tcgcgctcgc ctgcgcccat gcaaaaactc gaagggctcc aactttcttt tcgagtggaa
     6721 ccgtcccaaa ggatgaaacc gaatatccgc ctgttgcaac gaaaatacgg catccaggcg
     6781 aaggcgagtc tgacgtccca agccgttttc gacctgctgc tgggtcacaa atccttcgcg
     6841 aacgagaatt tcccccgcct tgaccttcgg taaacgctga agttcgtcga cgaatcgatg
     6901 atgctgttcg atcgacaaat ccccttgttc aagcaatact tcgccaagcc gtggaacagg
     6961 tatcggcgtg ccgacatcgc ataccatccc gtctcgcatc acaatccgat gacagcatcc
     7021 gcgataagga gcgtcgtggc cgatgagttc gagttcgcca gtggcttggg cacggtgcaa
     7081 tcgaccaagt acgtcgccaa gggtcgtgga ctgcaaacgg ccaggaagaa tcatggccaa
     7141 ccgatagaag ctcgacgcgc agcgggccaa ctcttccgtc gtccgcgctc caaaccctgc
     7201 cccccaaacc gggcgatccg ttatttcctc ggcattttcg acaaaaccaa cccggctggc
     7261 atcaatcatc cacactgaag ttgtcgtaag cgccagcttg ccctagcctg cggtcggtag
     7321 aagtcgcaca tgccttccgc gaagaagacg agcaacgttc ctattggaac gaccctcgac
     7381 gggaagtacc gtatcacgcg tgagatcgga cgcggtggga tggcttcgat ctatgaagcc
     7441 gtcaacgtgg atatcggcaa aaaagtagcc gtcaaagtcc tcaatcccga gctgaccaat
     7501 tccgccgtgg tggtcgaacg atttttgcgt gaggcaagag ccgccgccgc tgtaaaaagc
     7561 ccctatattt gcgacgtcta cgatgctggc aggctcgagg acggcagacc ctttctcgtg
     7621 ctggaactgc tcgaaggcga aagcttgtac gagcgaatgg tcaaagtacg gcgcttcgaa
     7681 cctgacttcg tggcccgtgt tttttcacaa gtggcgcgag gactcaccaa agcacacgaa
     7741 ggcaacatca tccatcggga tctcaaaccc gaaaacattt tcatcaccca taacgatgac
     7801 ggtgaggtcc tcgcgaaaat cgtcgacttc ggcctcgcaa aattctacgc ccccattgat
     7861 ggggatgcag cgcaacaacg actgactcga gaaggcgccg tgttcggtac accagcctat
     7921 atgagccccg aacaggtcaa aggccaaggc caggtggacc tgcgcgcgga cctttgggcc
     7981 gtcggctgta tggtctacga atgcctcatt ggcagaaccg tatgggcaac cgaacaaggc
     8041 gtcgccatga tctttgccca aatcgctacc gccgaaatcc ctgtcccttc actcattcgg
     8101 cgcgacttgc ccatcacctt cgataagtgg ttcgccaaag ctctcgccag agatcctgac
     8161 caacgattcc aatccgcaaa agaattggcc gacgaactcg tggcctctct cggacaaggc
     8221 cctccgtcga tcattctttc tcaaggcatc caggacatcg agcaagcggc cttgcagccg
     8281 ccgatctcct ccgatcatgc tttcgctcct acggaacgcc cgcctccccc cggattggag
     8341 gccgatcctc tggatgacaa cgctcccaca aggcaaatgg ccgttcctga cgaataccgc
     8401 gccatgccca cggatgccgc ggtcaaccgt tccgaatcgg cgaaaccacg cacttcgtat
     8461 cgcacatgga tcgctatcgc aggtgccctg ctcgtggcag ctattggcta tgtcgcgtgg
     8521 acgcaactgc ttcctgcccc tgctccagcc gttgtttcgt cccaacctgt aacccatccg
     8581 gtggttaccg cttccgcttc tcaaaaaact cccgaagtgc tcgtgccgac ccgaatcacg
     8641 aaagacttgc ctgactgggc aaaaaacatc gcttccgcgc aagagttgct gtcgaagggc
     8701 gacatcgagg gtgcttcgga aaccttccgc tctgctatcg acggaaacac cagtggtatc
     8761 cctcgggtga tgctcgaaaa cagtcgcttg gctgccaatt ccaaaggaac ctgtgaactg
     8821 ggcggcctgg gaagaccaag acctttcgaa ctcacgaatt ccatccgacg accggccatc
     8881 gtgttcacct cccatggccc gttggtgact tgggccgatg atcacgaagt tccaggacag
     8941 tggcatgctt atacgcagtt gctcgatgac gccttgcggg ctctcggaaa tcctgtcgat
     9001 gtcactcctg aagccatctc cgtgcaaact ccaaggctca tcgccatgga taaccaagtc
     9061 ctgctgttgt acagcgatca gcacggaaaa aatcccggtg tgttcgtgcg ccgcttgacc
     9121 acgaccggac gcattctcgg cgcccccctt caggttgctt ctgaacgtac ctccgttgtc
     9181 ttcccttcga tcacaaaaaa cccatgggga ggtttctggc tcgcctatgt cgaagacagc
     9241 gacaaatctc catcctccaa actcttcgtc caatctctca cgcatgatgt gaaactggat
     9301 ggcgaaagaa agctcatggc cgaatacgcc ttgcgctatg ggcttcatcg atcgcgaacg
     9361 aacgctccct ccgtggccgc gggcaccaac gccttgttgc ttagctatcg cgtggaacaa
     9421 ggacgcgacc atcgaaccgt cctgcaacat atcgctctcg aagaatccaa aattcctccc
     9481 ggcctttctg aagtcaatcc tccaaccctg gaccgggtcg tgggtgatgt gcgtgacctg
     9541 tcagaaggaa aaatccgact ctatccaccg atgatgcatt gtgaaaactc cgcctgcttc
     9601 gtcgtctggc gcgaggaacc ccgaggctcg aatgcagcgt atatcgatcc caccaccggc
     9661 accaccatct ggcgaaaacg ctttgcacac agaggtatgc aggtctccgt aggactcgat
     9721 gctgccggta acggactgct cgtctggtac caagatggac gcgtccacgt atcgcccatc
     9781 tcccgcgatg gtatcggtgt gccaagcgtc gtcggtcgcg tcaccggcga acaacctgta
     9841 ccctccgtct cgcgaggaaa aggccacggc gaatggctca tcgcgtggat cgattacgag
     9901 gctggacacc tcgaagccta tctcgcttcc gtcacatgca aatgatcatg gtcgacgagc
     9961 cgcatggtcg gtgacgagca tcccgaattg tgcgtgatcg agccaacgcc ccgcacaata
    10021 ttctgcttgt cgcgctatcc cttcgaaacg gaaaccaaga cgtccgatca ccgataagga
    10081 acggtaattg gtggtggccg ccgcgacgcg gattcggtgc atgtgatact tgtcgaaggc
    10141 ttcctgtatc aggatgcccg cagcttccgt catataaccg cgcccttgtg ccggctctcg
    10201 aagccaatag ccaaggtcgc agcacgcgtg catgggcgta caattctcca gcccaacgac
    10261 tcccacaagg gttcgcgttt cacgctcgcg gatcacgaag cgaatggctc gccccgaatc
    10321 ccaatccatc gcacacgctt ccgcaaaccg atgcatcgag gactcatcga cctgaaaggg
    10381 aacccacggc agccatggct cgagatactt gcgagattcc tggaccgcac accacaactc
    10441 tcgcgcatcg gagccttcga tcggaaaaag gagcaaacgc ttggagccaa tgggagccag
    10501 gcgtgccgaa ctgcgggctg gaagactgat catcaggggc gaaggtgtac tggacccctg
    10561 ttccgcgggg aagacctttc atgggcacgg cccgtgcgtg ccaagagact tggcggattt
    10621 ctcgatgcac accgattatt ctcgcctctc ccccaatgcc cgttgcatca gattcgaaaa
    10681 gaggatatcc cacgtcgcga atccgtttca tccctgcgca ctcgcccgaa accagcgtcc
    10741 atcctccttc ttcattccga ataattacct ttattatcaa ttgcttacat tttgatcgag
    10801 ccggccatcg atgactttcc tcccgggtaa gaaagcgtag ggtcgaaacg agtctcccct
    10861 gcgcaagtcc cgcgaaatta tcgccgtgat ccatggttca tgccacggcc ctgaactcca
    10921 gcctgtccca cgaaatcgca gggctttttg ggtaagccaa tgcctttcgt gggcccggag
    10981 ccctacgact cgcttcctcc accgctacag ggaactctca tcgccacagc gataggctac
    11041 ggcatgatcg cccataagcg agcccagggg cgaggggccg ctacggctgc acaggataac
    11101 ccagccggat ccggtacaga taatcccagg ggcctgtcgg attgttcgcc ggtgcgctca
    11161 cccccaccac ttccagcttg ttcgcgccga tgcgcatgat cgtgaaatga agctgtcctt
    11221 ggacgttatg aggcccctgg taaaacggcg tgcccaagct gaacgtggga attataatat
    11281 cttgataagg ggaggggcac gggaactctt tatgctcggg acggtggtgg ttatgaccat
    11341 gtatgtacgc aagcaccggc caattcggtg ataatttctt gccgatccct tcgaacgatc
    11401 cggttccgtg ctgggcatga tggatcaact tacaaaacga ctctttctct tccgcatcga
    11461 gggaacctaa accgtaatgc gccgtaatga cgatttgccg tttggcgctg ttttttctcg
    11521 aaagcaaaaa ctctttgaga aatctgcggg agtcatgagg atcgacgatg cggacattcg
    11581 tgttggtctc attgggtaag ttctccgctt tccatcctgg tttgacattc acattgacga
    11641 accagatatc atcccagcgc caggcataat gccctgtgcc cggtgtcgca tcatcgtaaa
    11701 gatctttggc atcccctggt cgatgcgctt gcgtgatgcg gtcgagatag tcgatcactg
    11761 gatggtggtc gggatttccc ggcaaggccg cgcggggaaa atcatgattg ccgtataagt
    11821 cgtagaccgg aaagctcagc ttgttctcgg ttccgcagcg cccgaatgcc tcccgataga
    11881 ccgtatattg ccggcagaca gcctgacccg ctggcacgga ttcgcttcct gtctgggtga
    11941 gatcgccagc gatgatgacc ccccgaacat gagaataagg cttgtcttcc tgggcaaaac
    12001 ttgccccttt cggccataca tgcgtgccga tgctgttgat cgcttgacgg atcaacaggt
    12061 tttgattcgg cgcgtcattc ttgttgacgc tacacccagg actcggatcc gccgcatggg
    12121 gatctccgaa cccaacgaag gtaatatccc gtttgtccgc ccctttccac cactgcatcg
    12181 gatcgaaaat gcacttgaat agatcatccg cctcggagca agaaccacaa tcctgaggac
    12241 aattaccgca gtcttcccca ggatcgcaga ctccattgcc gcaggtcggt gggcattgcc
    12301 cgcagtctac cgcacaattg ccgtaatcct ctcccggatc gcagactcca ttgccacacc
    12361 ccgacgagac ttccgaaaca atgtccgaaa aagcatccgg cagagcatcc ggcggccact
    12421 catcgaaaac atccgaattt ccgccagtat ccgttcccga ctcttgtctg gcatccgacg
    12481 atatcgctgc gtctcccacc gcggcgtcga ccttcgggtt cgtggtttcc ccctctgatt
    12541 ccgaactcga ggaacagccc cacgacaaaa ttcctgcccc gattccccag gcccatagcg
    12601 tgacccatcg tgcgcaatga cctcgcttcg ccatctcacc ctccattttc ccttcctatc
    12661 acgttcattc taccggaatc gcccgtttca ttccatacaa atattccgca acatgggaat
    12721 aatatccgtt ctaaaaacct gaaatacggt atttccccgg agaacctcgt tgtcaccatg
    12781 gagctatctc gattcttcgc gctcatcccg gaaagccgag gagccgggtt cgccttcgag
    12841 tacgctacgc ccccatcgat gaaacctggt cggcaacgca cggcgcagag ccgcttacgc
    12901 tttggccatc ccacgaaaaa aaactgcgaa atactctcga tcgacgcata cctccgcttt
    12961 cgcttggcac atgggcaggg cccgcacttt tcgtgatacc cgaactacga aataacgaag
    13021 atgaaaataa ctgcgtatgc ccaggaagag cccacccccc gcctgccatg cctatccccc
    13081 tatcatcgtt gtttccgttc ccgctttaaa acgaatctga accacaacgc tcgttgttcg
    13141 cgccatccaa tcgtgcaata attcatcgcg ccggcttcga ttcacgcatc gatgacgcag
    13201 accaaggact ggaggcaata tgcgtaggtt catcgccctg accctaacac tcggctttct
    13261 ggtttcaggc tgtggaacct ccaccgaagc ggactctccc ccaaccactc ccaatggcac
    13321 cgcctcctgc ggagactccg tttgcgatga gtccgaaagc tgtgaaactt gtccggaaga
    13381 ctgcggcgac tgcgaggatg gccccaactt ttgcggggat tttgtttgcg atgacgagga
    13441 agactgcgtg tcttgtcccg aggattgcgg tgactgcttc tcggaaatca cttgcgggga
    13501 cgatatctgc agcgagactg aatcctgttc gacatgcccg gaagattgcg ggcaatgcat
    13561 tgagtcgcat tgtggcgacg gacaatgctc gggtatcgag acctgcgata cctgccccgc
    13621 ggactgcggc ccctgcaaaa catcctgtgg cgatggcagt tgccaggagg atgaaaattg
    13681 cctgagctgc tctgccgatt gcggctcttg tgcccctggt cacctggata tctggagccc
    13741 tcgtcccatt gctccccagt tcgcggtggc tggcggcgcg attcaggccg aggtcaacgg
    13801 aaaagacact tggaatccga acgaatggta cgccgtcgtg cgcaatacct accgcacctg
    13861 gccttgcaaa gtcaccgcaa aatacggttc gatcgactac ggccgccgca agggatggat
    13921 cctggacatt caactgccca tatacgtccc acccgaattg ctcgaacttg ccattgccca
    13981 cggcgaggca ggcacgggct cgcgcatccg ctcggttcaa atggtcgcct ccttcgatga
    14041 ggacttctat atcattcatc aaaccgacca tcatatcgcc gccaaaaacg cgaaaagacc
    14101 tgatgggacg gtcgacctgg gaggcggacc caaaccaggg acctcccagg cgctcacctg
    14161 gggagccaaa gccatcaata tcatcaatcc gcgtttcgtc atgctcaccg gcgacaacat
    14221 attcgtcatt ttcgaagata agaattcttg gcgaggactc aaacggagcg ttgaaatcct
    14281 caattacttc aaagaaggaa tcaataccta tcaagtcgct accttggtga ccagcggcaa
    14341 ccatgacgtc ggcaccaata ttcatactac ccccgatcaa ctaaaatatc tcgaggaata
    14401 tgccaacatc ctgggcccca gatccttctc tcgtcgtatg ggcccgtttt atatcggtct
    14461 tttcgaatgg agccgatccc aattcaccga agccatgacc aaagactaca atgcctcctt
    14521 ccaagaccct ctcgtccaat ttcgtctcgt agggtcgcac ttcaact
//
LOCUS       DAOSOR010000066        14539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4173_length_14539_cov_4.492751, whole genome shotgun sequence.
ACCESSION   DAOSOR010000066 DAOSOR010000000
VERSION     DAOSOR010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 14539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14539
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4173_length_14539_cov_4.492751"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PK710_05450"
     CDS             complement(<1..345)
                     /locus_tag="PK710_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09194.1"
                     /translation="MPRRVAPIALLFAVTTTVFSACQLPVPPMDTAALPTTVTSAKPL
                     YIFTNPPSRPASSSALTIVDAPPSPASLPILSHVEMTRAAICPQKHCWRSWVVPRPTE
                     VVSPPTTPYPDFG"
     gene            462..1769
                     /locus_tag="PK710_05455"
     CDS             462..1769
                     /locus_tag="PK710_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOT09195.1"
                     /translation="MKKNYVLDTNVLLHDPQAIFRFEDNTVFIPIFVIEEVDQFKREG
                     SERGRNARTVSRLLDGLRERNGSLADGVPLDNGGFLRVVLPQTRGNLAVALDPTNADH
                     AILQTAIDVQKSFPDHRTVLVTMDTNLRIRADALGVTPENYESQRVQIDQLDTGAVEI
                     TVSDETIDTFYEKHELPLPPGMTLNPNVFVQLRGSSDAHGGLGRWDASRQRIMPLRSV
                     SDVMGIRPRNREQTFALDLLLDENIKLVTLVGKAGTGKTLLALAAALKRTIDEGAYSR
                     VLVSRPIIPLGRDIGFLPGSVDEKLNPWLQPIFDNLELLISASSNKKNLSMHKLIQEG
                     FIQVEPLTYIRGRSLPHQFLVVDEAQNLTPHEVKTIITRSGEGTKVVLTGDPYQIDNP
                     YVDSASNGLTIVTDRFKQEKVAGHVVLSRGERSELAELASNLL"
     gene            complement(1770..2282)
                     /locus_tag="PK710_05460"
     CDS             complement(1770..2282)
                     /locus_tag="PK710_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09196.1"
                     /translation="MPCCCDHRIRDLIDQARRKDVIAAWAIGLVCVVVGAMLFWRATD
                     TTGMVFGFLFFLVGAMGGLAVYRLDGGKLKRLWQRLQRQPELVVWLHESSVANRLRRG
                     EASVFVFFMDRTYVRVTLPKARASELMQWLIACCPQARQTRELSLSALLELELQWRKD
                     PAAFRSETST"
     gene            2491..4023
                     /locus_tag="PK710_05465"
     CDS             2491..4023
                     /locus_tag="PK710_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HOT09197.1"
                     /translation="MFKNHPKGLLAAALANMGERFGFYTMMAILVLFLQAKFGLSGTH
                     AGIIYSVFYFSIYILAFVGGLIADRTRRYKGTILVGLIIMALGYVLIAIPTPTPSPNP
                     TLFLVLTCVGLFTIAFGNGLFKGNLQALVGQMYDNETYAKMRDSGFSLFYMFINVGAL
                     FSPIAAVGIRNAWLTRQGFEYNSSLPELCHAHLHGTISHEASQRLTQLAAQVSTTPPT
                     DLTAFATNYLGVFAQGFHYAFGFSVVAMIISLAIYLSRKKGFPNPAEKKTKTESDTTS
                     IEMDRREVRQRLYALFAVFGVVIFFWFSFHQNGLTLTYFAKDYTDLSALHLDLGLVSI
                     HGAEIFQSINPLFIVTLTPIVMGLFGWLRARGSEPSTPKKIAIGMGIAAAAYLLMSLG
                     SWGLPSKSEVEAMGGLADSARVTPWLLVGTYFILTVAELFISPLGISFVSKVAPPQYQ
                     GIMQGGWLGATAIGNQLLFIGAMFYESVPIWLTWIVFVVACSLSMLTMLLMVRWLERV
                     AN"
     gene            complement(4066..6723)
                     /locus_tag="PK710_05470"
     CDS             complement(4066..6723)
                     /locus_tag="PK710_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT09198.1"
                     /translation="MPGESGDGPLTVVDGLAIEALRSLSFPVVLVSGQGMVLFINEAA
                     CHCRGAKAADCLNRPVWDVLFEQRDAERVRRTIVRCVAEGGEMSLEAMRSARGFVGRI
                     RWQWTCFQAGNDRAAMGVAFDREAAATIDNHLLLEETSARTLLKATFDLIFRIDRQGH
                     FLEVYAAHERLLFVSPEEIVGRTVRELIPEPVASQMMDSVYRALDTGSVDTIQYSLPV
                     APGHLYYEARQIAVGADEVLGVVRDITRRKLSEQALRDSEQQYRTLMRRASDAIVLMD
                     AVGQVVAVNHQACEMTGWREDALLSRNVAELLSVQGGASITSLLREWTEEPTRVDVRL
                     RRRDGGWLDIEMSASRMPDGRVQAILRDVTERKRAQDALERSERSFRLLIERAPNLIA
                     VHQAGRVVWVNDAFSKSFRLDKSELIGRRVVDLVHPDDWDAVLSRMRKQEETAVPSLV
                     SEERFLREDGTFFWTEAVGIPLDFQGVPSSIMIAHDVTERKRAEEERRVLEQRIQQTQ
                     TLQSLGLLAGGIAHDFNNLLMGVLGNASLALLDLPDDHPARHYLQRIEATAQRAAELT
                     KQLLAYSGKGQFLIAPIDLTKVVEEMNHLLTTVISKRARVQLELESDLPSIEADITQV
                     RQIVMNLITNASDALEGRDGTIVVRTGVMHADTAYLASTYIDERLDEGEYVFLEISDT
                     GHGMDRATIGRIFDPFFTTRFTGRGLGLAAVLGIVRAHRGAIRVHSELGHGTTFRVLF
                     PKMVRQVEKDLEIAQEHAASGSGTILLVDDESIVRTVARLVLERLGFSVVEAENGMEA
                     VEVYRTHLDRFRLVILDMTMPRMDGEEAYRALRSIEPKVRVLLSSGYDEREMVHRLEP
                     GDAPGFLQKPYTPSELTEAVFRMLGDSKE"
     gene            complement(6819..7799)
                     /locus_tag="PK710_05475"
     CDS             complement(6819..7799)
                     /locus_tag="PK710_05475"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOT09199.1"
                     /translation="MMRIEPSLTRDKLREALDKTVEATRFSALGEPYVGKVRDNYSTK
                     DGRRFIVVTDRVSAFDRVVGTLPFKGQILNRIAQWWFDRSRSVVPNHVIRPVDPNIVE
                     AVECKPLAVEMVVRAYLTGSTSTSIWTHYQKGQRNYCGHILPEGMRVHQRLPEPILTP
                     STKAAQGQHDVSASREQILAMGQVSAGDFDAAAAMAMKLFEFGRQVCESRGLILVDTK
                     YEFGKKPDGSLVVIDEIHTPDSSRFWFADTYEERMRKGEAPESFDKEYLRRWLKAEGF
                     VGQGPVPSIADEVRVEAAYRYAGIFEAITGERFEPDWQEPMQRMRKNLGL"
     gene            complement(7959..8282)
                     /locus_tag="PK710_05480"
     CDS             complement(7959..8282)
                     /locus_tag="PK710_05480"
                     /inference="COORDINATES: protein motif:HMM:NF013089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HOT09200.1"
                     /translation="MAWFILIFAGLFEIAWPVGLKISQMPGRWGMGLFIAVIGMAASG
                     LLLWLAQRSIPIGTAYAVWTGIGAAGTFVVGVLFFGDSLGLLRMISVGLIVAGIIGLK
                     LAHGV"
     gene            complement(8402..10258)
                     /locus_tag="PK710_05485"
     CDS             complement(8402..10258)
                     /locus_tag="PK710_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09201.1"
                     /translation="MRLGKVLAMVAAWGVATWGATASAQTLLVASPAGSAEPREIRVA
                     IASGPSQVTTWFSVQWEGAADTLSLIVPVVAGDRVDSSTDAWFEALEDCTYTRIRPPG
                     HFPKTYCENAVIPESSAVDRIGDAAHVPTVSPMGAPTWMTMADVVAFAEQMSVELSTP
                     ALTALADWDGKGYRFLRFTYEVEPGRATLRTLRIASTSSRASVPLLLSSAGAIPVWLR
                     VWLISDRRANPVEHSWSTLDSDEVTWSLSGKNPPTNYRNLLDKALDGTSWVVDHVSHE
                     AIYQRVGSGSAAPIPSLIETYFDRASGYGDTVSDVSACVERLSSMQSNEWMVGRVCAA
                     GALANVQGASCTEDTQPDQIAADDLRCGGIADDLALALAEKRPKDVWLTRWTGKLASW
                     SERMSDAFVGVEGPSQSSWMVSGQWDDDVCQKGGAGGSGAGSSSSPTGGYGGGSNWSP
                     SPYPDENVYETGDTDESSVLVEGSCWGSSVYRYDDGDESSFGDSSNDEDGACSKGSSD
                     SEEDQTCSGDSSDGGSDDSCSGDTSDSESVETCSGDSSDSSSSDACSGDSSADTCSGS
                     SKNSDCSMSPHKGRGGTGKRLPTSLFGVLLAASALSSRRLRKHEEIRHPLRW"
     gene            complement(10422..11777)
                     /locus_tag="PK710_05490"
     CDS             complement(10422..11777)
                     /locus_tag="PK710_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOT09202.1"
                     /translation="MATVGIYALGLLLWVAPTVTWAQQPSSAAEARRFASVFSSVAER
                     VRPSVVQLEVAVRDESANVFRWFKGVPSVTHPIRRGLGSGVVISQTGHIVTNNHVIEG
                     AIAISVRLHDGRLLPASLVGRDLATDLAVVKIDATGLRAAQLADSDAVKVGEWVMAIG
                     SPFGLGYTVTTGVLSAKGRGGLGVNSVEDYLQTDASINPGNSGGPLVNLDGHVIGINT
                     MIVGRGQGIGFAVSSNMAKRITEQLIAAGQVKRAWIGAGVQDLTPEIAAQMGVPAGSG
                     ALVNQVVPQGPAHLARIQAGDILASVDGKTVQDAQDLMREVLMHPVGQKVRIEVIRAG
                     KRYVTEVQLVERRESQAPPLPMQQRSQGGPSLGLSLRDIAYGSAPSSDKPPPMVAQVI
                     QVQAGSTADLAGLRPGDVIIQADGKSMPTMAQVADELKDGSVLLLVRREQGAFYAALK
                     R"
     gene            complement(11858..12679)
                     /gene="truA"
                     /locus_tag="PK710_05495"
     CDS             complement(11858..12679)
                     /gene="truA"
                     /locus_tag="PK710_05495"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOT09203.1"
                     /translation="MTCCVERGVLLTLAYVGGPFHGFVEQSNVRTVAGEVLGAVQQID
                     PHVKSLRGVSRTDAGVHARGQLAAFDSRLTVPPRGWLLAINRHLPDEIAVRHVVMVPC
                     GFDPRRYATLKWYRYSLLRDVRRDPFLDAVTWRIGGELDLHKARKEASDLVGTHDFAA
                     FRSSADERTSTMRTISRLTIESSIHDPRVIFIDVWGTGFLHNMVRIIVGTLVDVARGK
                     LDLGTMQRGLLTGDRSVLGITAPPQGLCLRHVELMDTAELGTEWPESGMQDTEAS"
     gene            complement(12679..13722)
                     /locus_tag="PK710_05500"
     CDS             complement(12679..13722)
                     /locus_tag="PK710_05500"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOT09204.1"
                     /translation="MSQYRRYSSSDRLKAVDFSLVESARRVIEGALSVVPGQKVLLVA
                     DEARIDLGNALLDACQQRNADATLLVLEYLQPRPHAALHPTIEQHLAQVEASVFIAGF
                     VEGEAQMRREFVGCTTSYQVRHAHMVGVSRRAMLSGLSTDPRRIATVASSVRGVLRPQ
                     SVFRVRSVTGTDLEVVCDPRHRWAEHSGIIRRGMWENLPTGELMTTPGNVHGVFVCDA
                     AMSEGFGSREGLLKDKPVTLEIRDGYVRDVQCSQGSLEREVRAWLLGGRFFDRVGIIS
                     LGINIGISSPIGEVICDQNIPGLHMSLGATYAEETRADWDADGQLLLTAHHQDVDLDG
                     RPLIRSGRYLNVR"
     gene            13886..14413
                     /locus_tag="PK710_05505"
     CDS             13886..14413
                     /locus_tag="PK710_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09205.1"
                     /translation="MERQSNTLLFVAGACVALIVGIVIAVRILSPEDARSASSLSSLR
                     LASSSAANQAPIAELEGQGPVQMLPITVEGKQGLLVRYTSDLISDPIQQKIASRVLDA
                     VKREAQKAQIEVIVVIAVERSQDGGSPLDAQTHTVAFRHLGEGVWVKVQDPHALAPDS
                     SALPPASVSPLTITH"
BASE COUNT         3418 a         4682 c         3530 g         2909 t
ORIGIN      
        1 accaaagtcc ggatagggtg tggtaggagg ggaaacgact tccgtgggac gcggaacgac
       61 ccaagatcgc caacaatgct tttgaggaca gatggccgcc cgggtcattt cgacatggga
      121 aaggatggga agggaggcgg gcgagggcgg cgcatcgacg atggtcagcg ccgaggagga
      181 agcggggcgc gacggaggat ttgtgaatat atacagtggc ttagcgcttg tcacggtcgt
      241 gggcaacgcg gctgtatcca tgggaggaac aggcagttgg caagcggaaa agaccgtcgt
      301 ggtcacggca aatagcagcg caatgggcgc aacgcgacga ggcatggggg ctagtgtagc
      361 cggatcttta atcggttcca tcgttcgtga cgataattgg ttgcttgcga atctggcgaa
      421 gccgagtacg gtcggcatgg gtagggcatc ggagataggg aatgaagaaa aattacgtac
      481 tcgacaccaa tgtgttgctc catgacccgc aagcgatttt ccgcttcgaa gacaacaccg
      541 tgttcatacc gatcttcgtg atcgaagaag tggaccagtt caagcgtgaa ggcagcgaac
      601 gaggcagaaa cgcgcgtacc gtttcgcggc tattggacgg tttgcgcgag cgcaatggct
      661 cgctggccga tggggttcct ctggacaatg gcggttttct tcgggtggtt ctgccgcaaa
      721 cacggggaaa tctggcggtg gcgctcgatc ccacgaatgc ggaccacgcc atcctgcaaa
      781 cggccatcga tgttcaaaaa agctttccgg accatcggac cgtgctggtg acgatggata
      841 cgaacttgcg gattcgagcc gatgcgcttg gagttacgcc tgaaaactat gaaagccagc
      901 gggtgcaaat cgatcagttg gataccggag cggtggaaat caccgtttcc gatgaaacca
      961 tcgatacgtt ctacgaaaaa cacgaacttc ctttgccgcc gggcatgaca ctcaacccca
     1021 atgtattcgt gcaacttcga ggctccagtg atgcccacgg aggtcttgga aggtgggacg
     1081 cgtctcgaca acgaatcatg cccttgcgtt ccgtatccga tgtgatggga atccgtccac
     1141 gcaaccgaga gcaaaccttc gccctcgatc ttctgctgga cgaaaacatc aaactcgtca
     1201 cgctggtcgg caaagccggc accggcaaaa ccctgctggc gttggccgcg gcgctgaaac
     1261 gcaccatcga tgaaggagcc tattctcgcg tgctggtaag ccgaccgatc atcccgctcg
     1321 gaagggatat cggatttcta ccgggctccg tcgatgaaaa actcaaccca tggcttcagc
     1381 ccatcttcga taacctggaa cttctcataa gcgcttcctc caacaagaag aacctttcca
     1441 tgcacaagct catccaagaa gggttcatcc aagtcgaacc gcttacgtat attcgtggtc
     1501 gaagcctgcc ccatcagttt ctcgtggtcg acgaagcgca gaatctcacg ccccacgaag
     1561 tcaaaaccat catcacccgc tctggcgaag gcaccaaagt cgtgctcacc ggcgatccct
     1621 accaaatcga caatccctat gtggatagcg cctccaatgg cctcaccatc gtcaccgatc
     1681 gattcaagca ggaaaaagtg gcgggacacg tggtgctatc gcggggcgaa cgaagcgaat
     1741 tggcggaact cgccagcaat ttgctttgat caggtcgacg tttcgctccg aaaagccgcc
     1801 ggatctttcc gccactgcaa ctcgagttcc aacaaagcgg aaagagaaag ctctctggtc
     1861 tgtcgcgcct ggggacagca cgcgatgagc cattgcatca attcggaagc ccgcgccttg
     1921 ggaagggtca ctcgaacgta ggtacggtcc ataaaaaaaa cgaataccga tgcctccccc
     1981 cgtcgtagac gattggccac cgatgattca tgcaaccaca cgaccaactc tggttgtcgt
     2041 tgtaaacgtt gccacagccg tttgagcttt cccccatcga gacgatagac cgcaagtccc
     2101 cccatggctc cgacgagaaa aaacaaaaac ccaaacacca tgccggtcgt atcggtcgcg
     2161 cgccaaaaca acatcgcgcc tacgaccacg caaacgagcc cgatcgccca cgcagcgatc
     2221 acgtctttgc gtcgcgcttg atcgatcaag tctcgaattc gatgatcaca gcaacagggc
     2281 acggggccat aggcttacga aagacagcaa ggcagtgcaa cgaataccgt gatggcatgg
     2341 caaaaccatc ttgtcgaagg ctcgtgggat tggtctttca ggaacattga catcgacccg
     2401 acttcgatgg ttctgtcagg ggcacgctgc gccgatttcc caaccatggt ggagtcgaga
     2461 ggtcgcagtg gccttgcaag gagacacacg atgttcaaga accaccccaa ggggttgctc
     2521 gccgcggccc tcgccaacat gggagagcgc ttcgggttct acaccatgat ggcgatcctg
     2581 gttctgtttc ttcaggccaa gtttgggcta agcgggaccc acgccggcat catctactcc
     2641 gttttctact tctcgatcta tatcctggcc ttcgtgggag gcctcattgc agaccgcacc
     2701 cgaagataca aaggtaccat cctggtcgga ttgatcatca tggccctcgg ctatgtgttg
     2761 atcgccattc ccacgccaac cccatcaccc aatccaaccc tatttctcgt gctcacctgc
     2821 gtcggattgt tcaccatcgc ttttggaaac ggtcttttca aaggcaacct gcaggcgttg
     2881 gtaggccaga tgtacgacaa cgaaacgtac gcgaagatgc gcgactccgg gttttcgctg
     2941 ttttatatgt tcatcaacgt gggtgcgctg ttttctccca tcgccgccgt gggaatccgt
     3001 aacgcctggc tcactcgaca aggcttcgaa tataactcca gcctaccgga actgtgtcat
     3061 gcccacctgc atggtaccat cagccatgag gcttcccaac gactgacgca gttggccgcc
     3121 caagtctcca ccaccccccc taccgacctg acagccttcg ccaccaacta tctcggtgtg
     3181 ttcgcgcaag gattccatta cgccttcgga ttttccgtgg tggcgatgat catatcgctg
     3241 gcgatctacc tttcccgcaa aaaaggattt cccaatcccg ccgagaaaaa aacaaaaacc
     3301 gaaagcgata cgaccagcat cgagatggat cgccgagaag tccgccaacg gctctatgcc
     3361 ctgtttgccg tgtttggcgt cgtgattttt ttctggtttt cgtttcatca aaacggtctg
     3421 acgctgacat atttcgctaa ggattacacg gacttatctg ctcttcacct cgatcttggc
     3481 ctggtttcca tccacggcgc cgagatcttt cagtccatca accccctgtt catcgtgacc
     3541 ttgaccccca tcgtcatggg actgttcgga tggttgcgcg cacgaggaag cgaaccgtcg
     3601 actcccaaaa aaattgccat cggcatgggc atcgccgcag ccgcatacct attgatgtcc
     3661 ctgggctcat ggggcctccc ttcgaagtca gaagttgaag ccatgggggg actcgccgat
     3721 agcgctcggg tcacgccgtg gctcctggtc ggaacctact tcatcttgac ggtcgccgaa
     3781 ttgttcatca gccccctggg catctccttc gtctcgaaag tcgctccccc tcaataccag
     3841 ggcatcatgc aaggcggctg gctgggcgcc accgccatcg gcaaccaatt gctcttcatc
     3901 ggagccatgt tttacgagtc cgtgcccatc tggttgacct ggatcgtttt cgtggtggcc
     3961 tgctcccttt ccatgctcac catgctcttg atggttcgat ggctcgaacg cgtcgccaac
     4021 tagcccctac gacaccccaa gcatgaacca agcctcggct tgccgttact ccttcgaatc
     4081 cccaagcatc cggaataccg cctccgtcaa ttccgaaggc gtataaggct tctggaggaa
     4141 gcctggagca tcgcccggct ccagccgatg aaccatctcc cgctcgtcgt atccgctcga
     4201 taaaagcaca cgaaccttcg gctcgatcga acgaagcgct cgatacgcct cttccccatc
     4261 catcctgggc atcgtcatat ccaagatcac gagacgaaaa cggtccaaat gcgtgcggta
     4321 cacctccacc gcttccatgc cgttttcggc ctccaccacg gaaaacccca aacgctccaa
     4381 caccaaacgc gcaaccgttc gcacaatcga ctcgtcatcg accaacagga tcgttcccga
     4441 accgcttgca gcatgttctt gcgctatttc taggtccttc tccacttgtc gaaccatttt
     4501 tggaaatagc acccgaaacg ttgtcccgtg acccaactca ctatggacac gaatcgcacc
     4561 acgatgagcc cgcacgatgc caagcacagc cgccaggccc aacccccgac ccgtaaatct
     4621 cgtggtgaaa aaaggatcga aaatccggcc tatcgtagcc cgatccattc catgccccgt
     4681 atccgagatc tccaagaaaa cgtactcccc ctcgtcgaga cgctcatcga tgtacgtcga
     4741 tgccagataa gccgtgtccg catgcatcac gcccgtacga accacaatgg ttccatcccg
     4801 cccctccaag gcatccgaag cgttggtgat gagattcatc acgatctgcc gcacttgggt
     4861 gatatcggct tcgatggagg gtaaatcact ttcgagttcc aactgcaccc ttgcccgttt
     4921 ggagatcacc gtcgtgagca gatgattcat ctcctccacg accttggtca gatcgatggg
     4981 agcaatgaga aactgacctt ttcccgaata cgcaaggagt tgtttcgtca attccgcggc
     5041 gcgctgcgcg gttgcctcga tcctttgcaa gtaatggcgc gcgggatgat cgtcgggaag
     5101 atccaacaag gccaagctcg cattccccag cacacccatc aacaggttgt tgaaatcatg
     5161 agcaattccc cccgcgagca gaccaaggct ttgtaacgtt tgtgtctgct ggatcctctg
     5221 ctccaacacg cgccgttctt cttccgcccg ttttcgctcc gtcacgtcgt gcgcaatcat
     5281 gatgctggaa ggaactccct gaaaatccag aggaatccct accgcctccg tccaaaaaaa
     5341 cgtcccatcc tcccgcagga aacgttcctc actgacaagg ctcggaacag ccgtttcttc
     5401 ttgtttccgc atccgcgaca gcacggcatc ccaatcgtcc ggatgaacca aatccacaac
     5461 ccgacgacca atgagttccg atttgtcgag ccgaaagctt ttagaaaacg cgtcgttgac
     5521 ccacaccacg cgtccggctt gatgcaccgc gatgaggttc ggagcccgct cgatcaacaa
     5581 ccgaaagctt cgctccgatc gctcgagggc gtcttgcgct cgttttcgct ccgtgacatc
     5641 ccgcaaaatc gcctgcacac gcccatccgg catgcgactc gcgctcattt cgatatccaa
     5701 ccacccccca tcgcgccgcc gaagacgcac gtcgacccgc gtgggctcct ctgtccattc
     5761 acgcaatagc gaagtaatgc ttgcccctcc ttgaacggac aacaactcag ccacgttgcg
     5821 agacaaaagc gcatcctccc gccagcccgt catctcgcac gcttgatggt tcaccgccac
     5881 gacttgccca acggcatcca tcaagacaat ggcatcggat gcgcgccgca tgagcgtccg
     5941 gtattgctgc tcactatctc gaagggcctg ttccgatagc ttgcgtctgg tgatgtcccg
     6001 aacgacaccc aaaacctcgt cggcaccgac cgcaatctgc ctcgcctcgt aatacaaatg
     6061 acctggcgcc accggaagag agtattggat cgtatcgacc gaacccgtgt ccagagcgcg
     6121 atacaccgaa tccatcatct gagaggctac cggttcagga atcaactcgc gcaccgtacg
     6181 gcccacgatc tcctcgggcg acacgaaaag aagacgctca tgggccgcat agacttccag
     6241 aaaatgccct tgtcgatcga ttcggaatat gaggtcgaac gtggctttca acagcgttcg
     6301 cgcactcgtt tcttccagga gaagatgatt gtcgatcgtc gcagccgctt ccctatcgaa
     6361 agccaccccc atcgccgccc ggtcattccc cgcctgaaaa caagtccact gccaacgaat
     6421 ccgtcccaca aagccgcgtg cggaccgcat ggcctcgagc gacatctcac ctccctcggc
     6481 cacacaccgc acgatagttc gccgtactcg ctcggcgtct cgctgctcga aaagcacatc
     6541 ccacacgggc ctgttcaagc aatccgcggc tttggcccca cggcaatggc atgccgcctc
     6601 attgatgaac agcaccatcc cctgccccga aaccaagacc acgggaaacg aaagacttcg
     6661 caaagcctct atcgccaagc catcgaccac ggtcagcggc ccatccccgg attcacccgg
     6721 catggcctgt ctccttcagg gattcgttca gattaaaccc agacaggatc gcaacccact
     6781 tgatcgtccg tgtcaaggtc cgtcgtggac ccaacccatc atagtcccag attctttcgc
     6841 attcgttgca tgggctcttg ccaatcgggt tcgaatcgtt cccccgtgat cgcttcgaaa
     6901 atcccagcat aacggtaagc cgcctccact ctcacttcat cagcgatgga cggaaccggc
     6961 ccctgcccca cgaaaccctc ggctttcaac cagcgcctca aatactcctt gtcgaaagac
     7021 tccggtgcct cacccttgcg catccgctct tcgtacgtgt ccgcaaacca aaagcgcgat
     7081 gaatccggcg tatggatttc atcgatcacc accaggcttc catctggctt ctttccgaat
     7141 tcatacttcg tgtccacaag gatcaagcct cgcgattcac agacttgcct accaaactca
     7201 aatagcttca tcgccatcgc cgccgcagca tcaaaatccc ccgcggatac ctgccccatc
     7261 gcaaggatct gttcccgcga cgccgatacg tcgtgttgcc cctgcgccgc cttcgtgctc
     7321 ggcgtcaata tcggctccgg caaacgttgg tgaacacgca tcccctccgg caaaatatgc
     7381 ccacaatagt ttcgctgccc cttctgataa tgagtccaaa tgctcgtcga tgtggaaccc
     7441 gtcaaatagg cccgcaccac catctccacc gcgagcggct tgcactccac cgcttcgacg
     7501 atattcggat cgactggacg aatgacatgg ttcggaacca cggaacgtga tcgatcaaac
     7561 caccactgcg ctattcgatt gagaatctgt cccttgaacg gaagcgttcc caccacacga
     7621 tcgaaggcac tcacccgatc ggtcaccaca atgaatcgac gtccatcttt cgtcgaataa
     7681 ttatcgcgaa ctttccccac atacggctcc cccaacgcgg aaaatcgcgt cgcctccacc
     7741 gtcttgtcca atgcctcacg caatttgtcc ctggtcaagg atggttcgat ccgcatcatg
     7801 agcacgcaag ctagccaagg cagatcacgg aagcaacgag cgtgcatcac catgagacat
     7861 cacggacaat cactcaaact cgaatgatcc gttttcggtg aacctggccc atcgtaccga
     7921 tgctttgaaa tcaacgccgc ggtccgctgc atcatggatc aaacaccatg agcaagcttc
     7981 aaaccaatga ttcctgccac gatcaaaccc accgaaatca tacggagcaa gccgagcgaa
     8041 tccccaaaaa aaaggacgcc aaccacaaac gttcccgccg caccaatccc cgtccatacc
     8101 gcataggcgg ttccaatcgg aatgcttcgt tgcgccaacc acaacaatag cccactggcc
     8161 gccataccaa tcacagcgat gaacaacccc atcccccatc gccctggcat ctgcgagatc
     8221 ttgagcccca ccggccaggc gatttcgaat agtcccgcga agatcaaaat gaaccacgcc
     8281 atcgaaacgg actattcgca gctcaacgcc cctttatcca gccctgtttc agattttcga
     8341 taaccgtgag cggatacgcc tccgctatcc cgttaccacc aaaggttctc gaaaaatggt
     8401 ctcaccatcg cagcggatga cgaatctctt cgtgtttgcg taaacgacga gacgacaagg
     8461 cgcttgccgc caacagaact ccaaatagcg acgtaggaag tctttttcct gtcccccccc
     8521 tgcccttgtg agggctcatc gaacaatcgg aatttttcga tgaaccacta cacgtatccg
     8581 cggatgaatc ccccgaacag gcatccgaac tcgatgaatc cgatgaatcc cccgaacagg
     8641 tttcaacact ctccgaatcg gatgtgtctc ccgaacagga atcatcgctc ccaccatccg
     8701 acgaatcccc cgaacacgtc tggtcttctt ccgaatccga cgatcccttc gagcaggcgc
     8761 catcctcatc gttcgatgaa tcgccgaagc tcgactcatc gccatcatca tagcgataga
     8821 cactgctgcc ccagcacgac ccttcgacaa gcacggacga ctcgtccgta tcgccggttt
     8881 catacacatt ttcatcagga tagggcgatg gggaccagtt cgaaccccca ccatatcccc
     8941 ccgtggggga agaagaggag cccgcaccgc ttccacccgc tccccctttc tggcaaacgt
     9001 cgtcatccca ttgcccactc accatccacg acgactggga cggcccttcc accccaacaa
     9061 acgcatcgct catcctctcc gaccacgacg caagctttcc tgtccaacgc gtaagccaca
     9121 catctttggg tcgcttttca gccaacgcca acgccaaatc gtccgcaatg cccccacatc
     9181 gcaaatcatc cgcagcgatc tgatcgggct gcgtatcttc cgtacaactc gctccctgta
     9241 cgtttgccaa tgcccccgcc gcacacaccc ttcccaccat ccactcattc gactgcatcg
     9301 aactcaagcg ctcgacgcaa gcagatacat ccgaaaccgt gtctccatag cccgaagcac
     9361 gatcaaaata cgtctcgatg agcgatggaa tcggcgcggc ggaccccgac ccgacacgct
     9421 gataaatcgc ctcgtgagaa acatggtcca cgacccatga agtcccatcg agcgccttgt
     9481 ccaaaagatt tcgatagttg gtaggtgggt tcttccccga caacgaccat gtcacctcat
     9541 cggagtcgag ggtcgaccaa gaatgctcga caggattggc tcgacgatcc gagatgagcc
     9601 aaacacgtaa ccacacggga atggctcccg cggacgataa caacaacgga acggaagctc
     9661 tgctcgacgt cgatgcgata cgcagcgtcc tgagcgtggc acgtcccggc tccacctcgt
     9721 aggtaaaccg caagaaccga tatcctttcc catcccaatc agcgagagcc gtgagagccg
     9781 gcgtcgatag ctccacggac atctgttcgg caaaagccac aacgtccgcc atcgtcatcc
     9841 aagtcggagc gcccatagga gaaacggtgg ggacatgagc cgcatcaccg atacgatcga
     9901 ccgcggaact ctcgggtatc accgcgtttt cacaatacgt tttcgggaaa tgaccaggag
     9961 gacgaattcg cgtataggtg cagtcctcga gcgcttcgaa ccacgcatcc gtactcgaat
    10021 cgacccgatc gccagccacg accggaacga tgagggacag cgtgtccgcc gctccttccc
    10081 attgcaccga aaaccaagtc gtgacctgag aagggccgga ggcgatggcc actcgtatct
    10141 cacggggctc agccgatccc gcgggagacg caaccaacaa agtctgagca gaagccgttg
    10201 caccccaagt ggcaacaccc catgccgcaa ccatcgcgag aaccttccca aggcgcatga
    10261 catctttcga gagcaattgc catgccgcgg agaaagctac cgactcaagt gtatctattt
    10321 gattttatta ttaatttatt tatcccggcg agatatgaga acggccgttc tctatggcgt
    10381 agcctcggag atgtggcatg ccaggcacag ccacaaaccc atcagcgctt gagcgccgca
    10441 taaaaggcgc cttgctccct tcgaacgagc aatagcacac tgccatcctt cagctcatcc
    10501 gccacctgcg ccatggtagg catggatttg ccatccgcct gaatgatgac atcaccaggg
    10561 cgaagcccgg caagatccgc tgtcgaaccg gcttgcactt gaatgacttg cgctaccatt
    10621 ggtggtggtt tgtcggacga tggagccgag ccataggcga tgtctcgcag ggacaagccg
    10681 agggaagggc ctccttggga acgctgttgc atgggcaatg ggggagcctg agactcgcga
    10741 cgctcgacca actggacctc ggtcacatac cgttttcccg cgcgaatgac ctcgatacgt
    10801 actttttgcc ccaccggatg catgagcact tcgcgcatca agtcctgcgc gtcttgcacc
    10861 gttttgccat cgaccgaagc aaggatgtcg ccagcttgga tacgcgcgag atgagcaggg
    10921 ccttgaggca cgacttgatt gaccagggca ccggaaccgg caggaacacc catttgagcc
    10981 gcaatttcag gggttagatc ctgaactccc gcgccaatcc acgcgcgctt cacctgcccc
    11041 gcggcaatga gttgctcggt gattcgcttg gccatattcg aagacacggc aaaaccaatg
    11101 ccttgtccac gaccgacaat catggtgttg atgccaatca cgtggccatc gagattcacc
    11161 aaaggccctc cggaattgcc ggggttgatc gatgcatcgg tctgaagata atcctcgacc
    11221 gaattgacgc cgagcccccc tcgccccttc gcgctaagca ctcccgtggt caccgtatac
    11281 cccagcccaa acggtgaacc aatggccatc acccattccc ccaccttcac cgcatcactg
    11341 tccgccagtt gagcagcccg caaacccgtg gcatcgattt tgacaaccgc caggtccgtg
    11401 gccaaatctc gccctaccaa cgaggcgggc aacaatcggc cgtcatgaag acgcacactg
    11461 atggcgatcg ccccttcaat cacgtgattg ttggtgacga tatgacccgt ttgcgaaatg
    11521 acgacgcccg atccgagtcc tcgtcgaatc ggatgggtca ccgaaggcac gcctttgaac
    11581 cagcgaaata cgttggcgct ttcatcgcgc accgcaactt cgagttgtac cacactaggg
    11641 cggactcgct cggcgaccga cgaaaagacg ctcgcaaacc ttcgcgcttc cgccgcagac
    11701 gacggctgtt gcgcccaagt caccgtggga gctacccaaa gcagcaagcc aagcgcgtag
    11761 ataccaaccg tagccataag atgatgagcg gttttcacag gacctccgtt cgatcaaaga
    11821 tgatgatccg tatccattgt ccatgttctt cacgaactta tgaagcctcc gtgtcctgca
    11881 tgccagattc cggccattcc gtgcccaatt ccgccgtatc catgagttcg acgtgacgaa
    11941 gacacaaccc ctgaggcggt gccgtgatac ccaatacact tcgatcaccc gtcagcaggc
    12001 cacgctgcat cgtgccaaga tcgagcttgc cacgagccac atccaccaac gttcccacga
    12061 tgatgcgcac catgttgtga agaaatcccg taccccacac atcgatgaaa atcacacgtg
    12121 gatcatgaat cgacgactcg atggtcagac ggctgatcgt tcgcatcgtc gaagttcgtt
    12181 cgtcggcgga agagcgaaac gcggcgaaat catgggttcc cacgagatcg ctcgcttcct
    12241 ttcgggcctt gtgtaggtcc aactcgccac cgattctcca tgtgacagca tcgagaaaag
    12301 ggtcccgacg tacgtctcgc agcagggaat accgatacca cttaagcgtg gcatagcggc
    12361 gtggatcgaa accacaagga accatcacga catgtcgaac cgcgatctca tcgggcaaat
    12421 gacgattgat cgccagcaac cagccccggg gaggaaccgt aagccgagaa tcgaaagccg
    12481 cgagttgccc acgagcgtga acacccgcat ccgttcggct cactccccgc aacgacttga
    12541 cgtgcggatc gatctgttgg accgcgccca acacttcgcc cgctacggtt cgaacgttcg
    12601 attgttcgac gaacccatgg aatggaccgc ccacatacgc aagggtgagc agcacgcctc
    12661 gttccacgca acaagtcatc agcgcacgtt gagatagcgc ccagaacgga tgagcggacg
    12721 tccatcgagg tccacatcct gatgatgagc ggttaacaat agctggccat cggcatccca
    12781 atcggccctg gtttcttccg cgtaggtggc acccaaagac atgtgcaaac caggaatgtt
    12841 ctgatcacaa atgacttcgc cgatcgggct ggagatgccg atgttgattc caagactgat
    12901 aatccccacg cggtcgaaaa aacgcccccc aagcaaccaa gcgcgaactt cccgctccaa
    12961 cgagccctgg gaacactgaa cgtcgcgcac atacccgtcc cgtatctcca acgtcaccgg
    13021 cttgtctttg agcaatccct cgcgagaacc aaatccttcg ctcatggcag catcacaaac
    13081 gaatacgcca tgcacattgc caggcgtcgt catgagttcg cccgtcggaa ggttttccca
    13141 cataccacga cgaatgattc cgctgtgttc ggcccagcga tggcgaggat cacatacgac
    13201 ctcgagatcc gttcccgtca cgcttcgtac acgaaacacc gattgtggcc gcaacacacc
    13261 ccgtaccgaa ctcgccaccg tggcgattct acgaggatcc gtcgacaatc cggatagcat
    13321 ggcgcgtcga ctcactccca ccatatgggc gtgacgcact tggtaactgg tcgtgcatcc
    13381 cacgaattca cgacgcatct gcgcttcccc ttcgacaaat ccagcaataa atacgctagc
    13441 ttccacttga gccaggtgct gttcgatcgt gggatgcaag gctgcatgcg gacgcggttg
    13501 aagatactcg agcacaagca acgtggcgtc ggcgtttcgc tgctggcaag catccagcaa
    13561 cgcattgccc aaatcgatcc gcgcctcatc cgccaccaaa agcacctttt gccccggcac
    13621 gaccgacaac gcaccttcga tcacacgtcg cgcagactcc acaagggaaa agtcgacggc
    13681 cttgagacga tccgaggaac tataacgtcg atattgactc acgatccgtg tcctatccca
    13741 tagctcagaa tcgtatgccg aggccagtgc ttgacgtatc ttcccacgtg gagaaagact
    13801 tcacaggctg tcattatccc tcgaacccat cccccaatcc atcatcgtgg gacctcgtta
    13861 cccaggtatg gcagcaagga tcctcatgga acgtcaatcc aatacgctac tattcgtggc
    13921 aggcgcctgt gtggccctca tcgtcggaat cgtgatcgcc gttcgcatct tgtcccccga
    13981 ggatgcgcgc tccgcttcct ccctatcctc gcttcgattg gccagttcct ccgctgccaa
    14041 tcaggctccc atcgccgagt tggaaggcca aggcccggta caaatgctgc caatcacggt
    14101 cgagggaaag caaggactcc tcgtccgtta tacatccgat ctgatctccg accccataca
    14161 gcaaaaaatt gcctctcgtg tcttggatgc agtcaaacgc gaagctcaaa aagcccagat
    14221 cgaggtgatc gtcgtcatcg ccgtcgagcg ctcacaagat ggcggctcac cactcgatgc
    14281 acaaacccat accgtggcat tccgtcatct tggagaaggc gtgtgggtga aagtgcaaga
    14341 cccccatgcc ttggcccctg attcatccgc gcttcccccc gcttccgtct ctcccctcac
    14401 catcacgcac tgagcgtatt acgccgcgat aatggcagaa agctcttgtc cgccatgggc
    14461 gcagacgtca tctctcgtat ccatcaaatc cccaataaaa tcaagtagat gcataaacga
    14521 gggcaggcgt acccccaat
//
LOCUS       DAOSOR010000067        14375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4239_length_14375_cov_4.671020, whole genome shotgun sequence.
ACCESSION   DAOSOR010000067 DAOSOR010000000
VERSION     DAOSOR010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 14375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14375
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4239_length_14375_cov_4.671020"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PK710_05510"
     CDS             <1..443
                     /locus_tag="PK710_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09206.1"
                     /translation="NPRWTPVWLMLLAWIVGTPRQAWADDDPWFGADKGLHFGASAVL
                     AASGYAVGTLVFEQRSSCAWLGAGVAMTAGVGKELYDAAGYGSPSLRDLVWDGVGTAV
                     GLGLAWSLDLLIRGQKRTETASPSTEAPVYPAPSISQAAWSLRF"
     gene            complement(464..658)
                     /locus_tag="PK710_05515"
     CDS             complement(464..658)
                     /locus_tag="PK710_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09207.1"
                     /translation="MPEYPTSAFSDYFGHRWHLFPISISRWEQGYPSLDLADPALGVR
                     SAGLLEKTDGIRYITPMISE"
     gene            682..1056
                     /locus_tag="PK710_05520"
     CDS             682..1056
                     /locus_tag="PK710_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09208.1"
                     /translation="MHPMTLAESSRTSQLPVPNAFSSTTYTLDTKALRRRGTALSANE
                     SDGILWAQHVAVLDARLGMSIRNVKSLLHTQNVVLVRQDCARHAVEAYRDSPTTTSYP
                     RFVLDPMLSLQSRMARSWALYG"
     gene            1217..1489
                     /locus_tag="PK710_05525"
     CDS             1217..1489
                     /locus_tag="PK710_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09209.1"
                     /translation="MSSYVGTEPLDAHDLIAIVISVFIPGVGHILLGQKAKGLLILAA
                     VVISLGVGYVLSCIIAVDALLVARTRKFRPVADFECFPEHQRFLGI"
     gene            complement(1502..2641)
                     /locus_tag="PK710_05530"
     CDS             complement(1502..2641)
                     /locus_tag="PK710_05530"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOT09210.1"
                     /translation="MYRVIFDARSVVPQSSGIGNYAATLLREMVAIDEAFSFTVLRHP
                     GSKHPLVDSDRVQEVEIVPGETKSVATLFRARAVMRLSGHDLYHSPADMVPPRVPCPF
                     VVTMHDMMWVEAPHLASNFWPSRLTMHLWYRGHYAHSIRGAIRVIAISQATADAITRL
                     YPRHASKIRVVRHGLDRQRFDPAKVPARSTLSGWVPESSPFLLAVGQGSPYKNHANIV
                     RAFLLATRDRPHHRLVLVRRFARVDFEMRRLLRQPEVRRKVLVYPFVPDDVLMALYGH
                     AQALVFASHYEGCGMPAMEAMALGTPVVASRAPAILEMTGDAALHADASDVAELASSI
                     RQVVEDEPTRNRLMAAGRDHIQQFRWQRCAQETLQVYREAIGRAG"
     gene            complement(2720..3568)
                     /gene="murI"
                     /locus_tag="PK710_05535"
     CDS             complement(2720..3568)
                     /gene="murI"
                     /locus_tag="PK710_05535"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOT09211.1"
                     /translation="MIGVYDSGYGGLSVLRQLRAVLPRHDFIYLGDSGRAPYGGRNSD
                     TILDFSEQCVERLFEEGCRLVVVACHTVSCVALRHLQRRYVPNAEAPRRILGVTIPAA
                     EAAVAQSTGHIGVIGTTRTIGSTTFSLEIAKLGPHRVTGHAAPLLAPIVEEGWADTDI
                     ARLAVQRYVDRFEDIDVLVLACTHYPLLLPAFQKAVGPGVQVLDPSPHVARRLVDWLT
                     RHPGFTEPGQGKLSLLCTGDPEQFRIHGEKFLGGELPRVQHIAEEQGRLAHRDRMQVV
                     IGQVVR"
     gene            3710..4672
                     /locus_tag="PK710_05540"
     CDS             3710..4672
                     /locus_tag="PK710_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOT09212.1"
                     /translation="MNPIVSCQSTITDVVVYGRGAIVTRTIHVPDGLADGIVDIHLDG
                     LPALADAGGIRAEIAAKDRRIVALQSSLTIPEQAPIPGTSVQRVEELAARIERWQDER
                     SVVQAERERLEGIEPKPPRRRALYEAPRQHLDDSLAAGGLLDDILARLDARLLDLEAS
                     LVQARKDLEAARLADSQAESSQREGVGHPTRRVHLRLDGNGPVEAIRLMYAVAPARWW
                     PVYTLRLTEKGSATFWMEALVAQLSGENWKNARLSLCTAGLIDDIRLPVLASMRLGRA
                     QPVEHRGYRLAPPDLDRLFFLLMSLFRRRPTGAPRCPRSLWGAA"
     gene            4825..5856
                     /locus_tag="PK710_05545"
     CDS             4825..5856
                     /locus_tag="PK710_05545"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOT09213.1"
                     /translation="MPKPAFRAMAAKAPPRPAAVAAASPGAGAYLPNQAELDGGGVDG
                     APSVPMEAALDVEQQSLEPPQPVEPADAWLDFDSLRLGSGFDREHRGRLYRQSDSALA
                     SRQQRALSRIEALSPSALVQDPYENRGAFDHRFDAQGGVEVPSDGRAHRITLSSASAP
                     ARFHYRTVPVESAEVYRELVLTNPLQAPILDGPVDVYLDGSLLTTTRVERIDEGGSFR
                     VGLGVEERIRVARNVRVEEETTGLLGGTTSIQHSVQIDLRSSMGSAVSVEVLDRIPDT
                     DDKNIEIKVGKFSQPGIKYTQMDRGTPIRGGYSWSVELSAGGSETLTYSYRILLPSKN
                     EIVGGNRRD"
     gene            5849..7435
                     /locus_tag="PK710_05550"
     CDS             5849..7435
                     /locus_tag="PK710_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HOT09214.1"
                     /translation="MTDSNFQPESWSEHPTVQAPAVQVTFFEDRAEVVRRASVHMPQG
                     RSWVTLLGPGVLLDDASVVVQVIGQKARVLGSRVLRHKRSATALPSHELEELEKAAQQ
                     AQQEHKRLLQALDRVQRKRQRSSAWLDLWSRALPRAPQPSPQNLEDWKAAIEKIEAQM
                     TEALDRTSELGRALQQAKRAEKQANLRLEAGKRVSPDYRAALEVQIESPADQTIEIEA
                     RYRTACALWRPEHLARLQTTPEGELEVEVQTVATAWQATGEVWEGVRCKFSTARPAHA
                     ASPPLLSEDLLVSRRKTEQERKYVRVEQRDEQVATVGIDRGTRQVEEMPGLDDGGEPV
                     SFEPEGPCSIASDGHPFRVELSSRKLPCKLDRIAYPELGEAIHFRMTSTWDGKMPLLA
                     GPVWIARQGAMIGRSKTSFVGQGEPFELGMGVDDGLRVRRTVEEKRDTTLVMGKQRIT
                     RTIKVFLRNLSDSERALRVVERYPVSEIEDVNVELVSYREGKPEPRDGFVHFDVTVPG
                     HGTKELVLEVRIEASSRVVM"
     gene            complement(7640..8959)
                     /locus_tag="PK710_05555"
     CDS             complement(7640..8959)
                     /locus_tag="PK710_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOT09215.1"
                     /translation="MRTKAVFTLLTLLSGLTVLACSESSDGPVESPKPAAEGGAGGQD
                     GGADVSSGGDTGVDVDAKTEGGGGKDVGAGGEGTEGGLVDAQPDTPADAPPEEPLTAK
                     RAAAEMGYGFNLGEFFESTKETPRTFEVAKKKIDAYYAMGFRNLRIPITWTEKVNGTR
                     LVHDPLVGDVDHQHPRLAEITKVIDYALSLPGLYVVINAHHEKELKEYSRDTVLQRLW
                     ADITEMYADRDHRLLFEFLNEPHRSGQNEMPGPDLVRMTGMAYEEVRARDPERIVIIG
                     GNRVFYAAEMGNVWKNLDKVGGGNDPFVMATFHHYNPWDFCGDHKYPYTYPWTKEEQE
                     KPMNTMKKWAEGDGQGMPVYIGEWGVGWKSGESKPPLRCNNIREWYSTFHRDSAAPRG
                     QPTAVWDDGGWFKVFDYQTGEFDNNLAECIVGPCAWDGTGRYDGCGK"
     gene            9256..10413
                     /locus_tag="PK710_05560"
     CDS             9256..10413
                     /locus_tag="PK710_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09216.1"
                     /translation="MKMNHAGIGLIAGLGLALLFVGCSDDEESVDGTQNPAGGGGSGG
                     TAVDASAEGAAPDAGVAGTGGKPTSDGGGPEGGEPDSGEPEAGNGGAGGAAPANFIPS
                     NRLNCDGLTDGEAAVGISWGNPGKGENADTGTYGDTSIKLPGRTSSCKLAIKKGSTGR
                     PGDGADSTEGDFGFFMGLSPDWKTLVGEGEELWVGVRIRFPSSFSFYTNTGFLKFIRL
                     EYVQPDPNHPTYGRVDWLIENGLGDAQIGWRYGDENQDFGLDKLGGELFVLDEWNWAE
                     VYFKASADPAKSVTRLWVNGFMQYEITEGKNVRNRTSAGTYETSTTKKMLPTLPAATT
                     ALESVMLFTYWNGGAPKTQSCNVQDVIIHDNGADLAAKDEFGNKMIGPDAF"
     gene            complement(10757..11920)
                     /locus_tag="PK710_05565"
     CDS             complement(10757..11920)
                     /locus_tag="PK710_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09217.1"
                     /translation="MRNNYVETGLIAGLTLAFFLAGCSNGDDESTDGPKNPAGNGGSG
                     GAGADANADGTMPEAGAAGSGGKPATDSGTTTDGETDSGNGGEAGTAPDGGGGGAAGA
                     GSGGAAGASPEVTIYFTEEFEDAEYKTRGWYESLPKTLSSDSHSGSHSLECTYNKGQP
                     DCSGQGAGRHLFPETDAVYLGYWVKYSSDFVGSGKPYHPHEFYFLTNKDDKYEGPAFT
                     HLTLYLESHWESASAGALWVGFQDGKNIDQSRRGEDLIAETENRAVAGCNGLPSGYSG
                     SCYNAGSGVYVNGVTLASPTVLTPQNKNDWNHVEYFAKLNSISAGRGQPDGILRVWVN
                     GAMVFERTNAILRTAQHADMKLNQLLFGPWIGDGSPIKQSFLIDDLVVASGRP"
     gene            12252..12416
                     /locus_tag="PK710_05570"
     CDS             12252..12416
                     /locus_tag="PK710_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09218.1"
                     /translation="MTHGHDTHSESTLFRDVFLLDAVVLGVSAAGACRLGESGTWRSS
                     PSETEQHPVQ"
     gene            12444..13667
                     /locus_tag="PK710_05575"
     CDS             12444..13667
                     /locus_tag="PK710_05575"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT09219.1"
                     /translation="MNRMGWLLVLVVATVPTIGSCRLGPSLDDYRSGSGGGPGVGGAA
                     GASGGASGSEGGAGGIGGASGGEGGVGGIGGASGGEGGVGGIGGAAGGEGGASGVGGS
                     GGAGGIAYGPSCEGLEEICGPAGQKQNCCENALVVGGSFPMGRCEDPSESCTDSFEKG
                     DPNELPEHTATVASFVLDRFEVTVGRFRRFLEAYPMSKPSSSAGAHPLIPETGWRSAW
                     DSKLPADKDKLMVALNCNAATKTWTDSPGSFEAMPINCVDWYLAFAFCAWDGGRLPTE
                     AEWEYAAAGGEENRRYPWGATAPDANSANYVASDDKPFVVVGSYPDGMGRWGHYDMAG
                     SVWEWVFDYYGTAWYGEGGLNCDNCANLTSSWSGHRIRRGGGYKSAAGELRAVSRASD
                     IGASPRYGLRCARSP"
BASE COUNT         2917 a         3933 c         4425 g         3100 t
ORIGIN      
        1 cgaacccacg ttggaccccc gtttggctca tgcttctggc atggatcgtc ggaaccccac
       61 ggcaagcctg ggcagacgat gacccatggt tcggtgccga caagggattg catttcggag
      121 cctcggcagt tctggccgca agcggttatg ccgtaggcac gctggttttc gaacagcgct
      181 cgtcttgcgc ctggcttggc gcgggggtcg ccatgaccgc tggcgtcggc aaagaactct
      241 acgacgccgc gggctacggt tccccttcct tgcgggacct cgtatgggat ggcgtgggaa
      301 ccgccgtggg cctcgggctg gcatggtccc tggatttgct cattcgcgga caaaaaagga
      361 cagaaacagc ttcgccatcc accgaagcgc ccgtgtaccc cgccccttcc atcagccagg
      421 ccgcatggag cctgcggttc tgatgcagga cacacgtcac ctgtcattcg gaaatcatgg
      481 gtgtgatata ccggatccca tccgtttttt cgagcaatcc cgcgctacga acgccaagag
      541 ccggatcggc cagatccaga ctgggatatc cctgctccca cctcgaaatc gaaatcggaa
      601 aaagatgcca ccgatgaccg aaataatcgc taaatgctga tgtaggatac tctggcaccg
      661 ttcttggact agcgagacac catgcacccc atgacgctgg cggaatcgtc gagaacaagc
      721 caactcccgg tccccaatgc tttttcatcc acgacgtata cgttggatac caaggccctt
      781 cgtcgaagag gcactgccct ttccgcgaat gagtcggatg gtatcctgtg ggcgcaacac
      841 gttgccgttc tcgatgcgag attgggtatg tccattcgaa acgtgaagtc gctgctgcac
      901 acccagaacg tcgtgctcgt tcgccaagac tgtgcccgac acgccgtcga agcctatcgt
      961 gattcgccta caacgacgtc ctacccacga ttcgtgctgg atcccatgct ttctttgcaa
     1021 tcacggatgg ctcggagctg ggccttgtac ggctagctag agaatttccg ctctcaaggg
     1081 ggtagcttgt ttgtaatcat ttgaaattat tgattaaatt tgaattcctt ccacggcttc
     1141 ctccccatcc ataggtttcg aaattaattt cttcgctcga tcgaattggc atcacctctt
     1201 cgcattggag cgagccatgt catcttatgt tggtactgaa cccctcgatg cccacgacct
     1261 catcgccatc gtcatttcgg tatttatccc cggcgtgggg catattttgc ttgggcaaaa
     1321 ggccaaaggg ctgctcattc tcgccgccgt ggtgatttct ttgggagttg gctacgtttt
     1381 gtcttgtatc attgccgtcg atgccctgtt ggtggcgcgc acgcgcaagt tccgcccagt
     1441 ggcggacttc gaatgctttc ccgagcacca acgttttttg gggatttgaa agacttggcg
     1501 gtcaaccggc tcgtccgatg gcttctcgat acacctgtag tgtttcctgg gcacatcgtt
     1561 gccagcgaaa ttgttggata tggtcgcgtc ccgcggccat gagccggttt cgcgtcggtt
     1621 cgtcctcgac cacctgccgt atgctcgatg ccagctcggc aacgtcggaa gcatccgcat
     1681 gcaacgcggc gtctccggtc atttcgagga ttgcgggggc acgcgacgcg accactgggg
     1741 ttccgagcgc catcgcttcc atcgccggca tgccgcatcc ctcataatga gaagcaaata
     1801 caagagcttg cgcatggccg tacaaagcca tgagcacgtc gtcgggaacg aaaggataga
     1861 ccagcacttt gcgacgcact tccggttgac gcagcaaacg gcgcatttcg aagtcaaccc
     1921 gagcgaaacg ccgcacgagc accagccgat ggtggggcct gtcgcgggtg gccagcaaga
     1981 aggcacgaac gatattcgcg tggtttttgt aggggcttcc ctgtccgacg gcgagcagaa
     2041 acgggctcga ctcagggacc cagccggata aggtgcttcg ggcaggaacc ttcgccggat
     2101 cgaaacgctg cctgtcgagg ccatggcgaa ccacccggat cttcgaagca tggcgtggat
     2161 acaagcgcgt gatcgcatcg gccgtggcct gggagatcgc gatgaccctt atcgctcccc
     2221 gaatcgaatg cgcataatgc ccgcgatacc atagatgcat ggtgagccgc gaaggccaaa
     2281 aattagaagc taagtgtggg gcttctaccc acatcatatc gtgcatcgtc acgacgaaag
     2341 ggcaggggac gcgcggcgga accatatccg ccggggaatg gtaaagatcg tgcccggaca
     2401 gccgcatcac cgcacgagca cgaaagagcg tggccacgga tttcgtttct cccggcacga
     2461 tctcgacttc ttgaacccga tcgctgtcca ccaacgggtg tttgctgccc ggatggcgca
     2521 gcacggtgaa agaaaacgcc tcatcgatag ccaccatttc acgcaacaag gtggcggcat
     2581 aattgccaat accactggat tggggcacca ccgaacgagc gtcgaaaatc acacgataca
     2641 ttgacaatga cccatagtag ggcaggagtt tttcatgggc tacggagcca tgggaacatt
     2701 gtggccacgg gcaagattct caacgtacga cctgaccgat cacgacttgc atgcggtctc
     2761 gatgcgccag tcgtccttgt tcctcggcga tgtgttgcac gcggggcagc tcaccgccaa
     2821 ggaacttttc accatgaatg cgaaattgtt cgggatcccc cgtacacaac aggctgagct
     2881 tcccttgtcc cggctcggtg aatcccggat gccttgtcaa ccagtcgacg agccgccgtg
     2941 ccacgtgagg gctcgggtcg agcacctgga cccctggtcc cacggctttc tgaaatgccg
     3001 gcagcaacag gggataatgc gtgcaggcca gcaccagcac atcgatatcc tcgaatcgat
     3061 cgacatagcg ctgcaccgcc aacctggcga tatccgtatc ggcccagcct tcttccacga
     3121 taggcgcgag aagaggagcg gcgtgacctg ttacccggtg tgggcctaac ttagcgattt
     3181 ctaaagaaaa tgttgtggag cctatcgttc gtgtggtgcc gatgacgccg atatggccgg
     3241 tgctttgcgc taccgcggcc tccgcggcag gaatggtgac gccgaggatg cgacgcggcg
     3301 cctccgcgtt cggaacatag cgccgttgta agtgacgaag ggccacgcaa gaaaccgtat
     3361 ggcatgccac caccaccagc ctgcagcctt cctcgaaaag ccgctcgaca cattgctccg
     3421 aaaaatccag gatcgtatcg ctatttcgtc ccccgtaggg cgcgcgtccc gaatctccca
     3481 aatatatgaa atcatgccgc ggcaataccg cccgaagctg acgcagcacc gataatccgc
     3541 cgtagccgga gtcatatacg cctatcaagg ggtacctcag atcgcgttga accccttaac
     3601 gccgaaaccg tcaagttttt ccctgacaac ggaggcagga gcaccgtccc caacccaggc
     3661 ttcccggctc cgccttgcat ccgtgccgtg attatggaaa cgtcgccccg tgaacccaat
     3721 cgtctcctgc caaagcacca tcaccgatgt ggtcgtctat ggtcgaggtg cgatcgtcac
     3781 ccgaaccatc cacgttcccg acggtcttgc cgacggcatc gtcgacattc acctcgacgg
     3841 cctccctgcc ctggccgatg ctgggggaat ccgggccgaa atcgccgcga aagaccgtcg
     3901 aatcgtggcc cttcagtcga gtttgaccat accggagcag gctccgatac ccgggacttc
     3961 ggtgcagcgg gtcgaggagc tggcagcgcg gatcgagcga tggcaggacg agcgcagtgt
     4021 cgtgcaagcc gagcgggaac ggctcgaggg gattgagcca aaacccccga ggcgtcgtgc
     4081 cctgtacgaa gctccacggc aacacctgga cgatagctta gccgcgggcg gtttgctcga
     4141 tgacattctg gcgcgtttgg atgcgcggtt gctcgatctg gaggcgtctc tcgtccaggc
     4201 tcgtaaagac ttggaggcag cgcgtcttgc ggattctcaa gcggaaagca gtcaacggga
     4261 aggggtgggg catccgacac gccgggtgca tcttcgcctc gacggcaacg gtccggtcga
     4321 ggccatacga ttgatgtacg cggttgcgcc ggcccgatgg tggcccgtgt atacgttgcg
     4381 gttgacggaa aaggggtctg cgacgttttg gatggaagcg ctggtggcgc agctatcagg
     4441 agaaaactgg aaaaacgctc gcctctcgtt gtgtacggcg ggtttgatcg acgatatacg
     4501 tcttccggtg ctggcatcga tgcggttagg cagggcgcaa ccggtcgagc atcgggggta
     4561 ccggctcgca ccgccggatc tcgatcgcct attctttctt ttgatgtctt tatttcggcg
     4621 acggccgacg ggggcgccca ggtgccctcg gtcgctttgg ggagcggcat agggtttgga
     4681 atgcccgcaa tgggtatgcc tttgccagct ccaccgccgt ctccacagcc gtctccagcg
     4741 ccggtggcgc tgatggaaga atcgaagtat atacaaggga tacctcggtc tcccgcagcg
     4801 gatgggaggg aacgagcgcc ccaaatgccg aaaccggcct ttcgggctat ggcggcaaag
     4861 gctccgccca ggccggcagc ggtggccgct gcctctccgg gcgcgggggc atacctgccc
     4921 aaccaagcgg aattggatgg gggcggcgtc gatggtgcgc ccagcgttcc gatggaggcg
     4981 gcccttgacg tggaacagca atctctcgag cccccgcagc ccgtcgagcc cgccgatgca
     5041 tggctcgact tcgatagttt gcgtcttgga tcgggttttg acagggagca tcgaggccgg
     5101 ctgtaccgtc agagcgatag cgccctcgcg tcgcgccaac agcgtgcgct gtcgcggatc
     5161 gaggcgctca gcccttcggc gttggtgcaa gatccttacg aaaatcgagg ggctttcgac
     5221 catcgtttcg acgcccaggg tggtgtggaa gtgccatcgg acggccgagc acatcggata
     5281 acgctgtcgt cggcttccgc tcccgcgagg tttcattatc gaacggtgcc ggtcgagtcg
     5341 gcggaagtgt atcgcgagct ggttttgacc aacccgctcc aggctcccat tctcgacggc
     5401 cctgtggacg tgtatctgga tgggtcgttg ctgacgacca cgcgggtgga gcgaatcgac
     5461 gagggcggtt cttttcgggt cggtcttggg gtggaggaac ggattcgtgt ggctcgtaat
     5521 gttcgtgtgg aggaagaaac cacgggtttg ctagggggaa cgacttcgat ccagcattcg
     5581 gtacagattg atttgcgctc ctctatgggt agtgcggttt cggtggaggt gctcgatcgc
     5641 ataccggata cggatgataa gaatattgaa atcaaagtag gaaagttttc gcagccagga
     5701 atcaagtata cgcaaatgga tagagggact ccgattcgtg gagggtattc gtggtctgtc
     5761 gagctttccg cggggggttc ggaaacgctg acctacagct atcgcatcct gttgccgtcg
     5821 aaaaacgaga tcgtaggagg caatcgccgt gactgattcc aacttccaac ccgaatcgtg
     5881 gagcgagcat ccgacggtgc aagcgcctgc ggtgcaggtc acgtttttcg aggaccgcgc
     5941 ggaggtcgtt cgccgtgcaa gcgttcatat gcctcagggc cgcagttggg tgaccctgtt
     6001 gggacccgga gtacttctcg acgatgccag cgtggtggtc caggtgatag ggcaaaaggc
     6061 gcgcgtgctc ggttcgaggg tgcttcgcca taaacgctcc gctaccgcgc tgccctccca
     6121 tgaactcgag gaactcgaaa aagccgcgca acaagcacag caagagcata aacggctttt
     6181 gcaagcgctc gatcgcgtcc aacggaagcg tcagcgctcg agcgcctggc tggacctgtg
     6241 gtccagggcg ttgccgcggg ccccgcaacc atcaccgcaa aacctcgagg attggaaggc
     6301 ggcgatcgag aagatcgaag cgcaaatgac ggaggcgctc gatcggacca gcgagctggg
     6361 tcgcgcgttg caacaggcta agcgggcgga aaaacaagcg aatttgcgat tggaagcggg
     6421 aaaacgcgtc tcaccggatt accgcgcagc cctcgaggtg cagatcgaaa gcccggcgga
     6481 tcagaccatc gagatcgaag cccggtatcg cacggcctgc gcgctgtggc gtccggagca
     6541 tctggcgcgg ttgcagacca ctccggaagg cgagttggag gtcgaggtgc agacggtggc
     6601 gacggcctgg caggctaccg gcgaagtatg ggaaggggtt cgatgcaagt tttcgaccgc
     6661 tcgccccgcg catgcggcgt cgcccccgtt gctttcggaa gacctgctcg tttcgcgccg
     6721 aaaaactgaa caagagcgga aatacgtgcg ggtcgagcag cgcgacgaac aggttgcgac
     6781 ggtgggaatc gatcgtggta cgcggcaggt ggaagaaatg ccgggcttgg acgatggtgg
     6841 ggagcccgtg agtttcgagc ctgaagggcc gtgtagcatc gcgagcgatg gtcatccctt
     6901 tcgggtggag ctgtcctctc gaaaattacc ttgtaaactc gataggatag cgtatccgga
     6961 gctgggggaa gccattcatt ttcggatgac ttcgacctgg gatggcaaga tgccgctgct
     7021 ggcggggccg gtgtggattg ctcggcaagg ggcgatgata ggccgctcca aaacgtcttt
     7081 cgtgggtcag ggagagccct tcgaacttgg aatgggcgtg gatgatggcc tgcgcgtgcg
     7141 tcgaaccgtc gaggagaagc gggatacgac gctggtgatg ggaaagcaga gaatcacacg
     7201 tacgatcaag gtgttccttc gcaatctcag tgattcggag cgtgctttgc gcgtggtgga
     7261 gcgctatccg gtgagcgaaa tcgaagacgt gaatgtggag ttggtttctt atcgggaagg
     7321 gaagccggag ccgcgcgacg gttttgttca ttttgatgta acggttcccg gccatggtac
     7381 gaaagagctt gtgttggagg tgcggatcga ggcttcttcg cgggtggtga tgtgatggct
     7441 gatagaaggg gttaatcctg tgggcttcct tgtttcgtgg agccgcggaa aacaggataa
     7501 gtatgtgtaa tcaaagctga atatgggcta ttggagagag gatcctggcc gctgtgcttt
     7561 ggggttgttg aggatccaga ggtgggaatt ggggaactag caactagcaa ctagcaacta
     7621 ggaattgggg ggccaaaaac tacttaccgc agccgtcgta ccgcccggtg ccgtcccacg
     7681 cgcacggtcc cacgatacac tcggcaaggt tgttgtcaaa ttcccccgtc tggtaatcaa
     7741 agactttgaa ccatccgcca tcgtcccata cggctgtagg ttgtcctcgt ggcgcggcgc
     7801 tgtcacgatg gaacgtcgaa taccattcac ggatgttgtt gcagcgaagc ggtggtttgc
     7861 tttcaccgct tttccatccc acgccccact ctccaatata aacgggcatt ccctgaccat
     7921 ccccctccgc ccattttttc atggtgttca tcggcttttc ctgctcctcc ttcgtccagg
     7981 gataggtata cggatacttg tgatccccac agaaatccca cggattatag tgatggaagg
     8041 tcgccatcac gaacggatcg tttccaccac cgaccttgtc gaggttcttc caaacgttgc
     8101 ccatctcggc cgcgtagaac accctgttgc ccccgatgat gacgatccgc tccggatcgc
     8161 gcgcgcgcac ctcctcgtac gccatgcctg tcatacgcac caaatcgggc cccggcatct
     8221 cgttctggcc gctgcggtgc ggttcgttga gaaactcgaa cagcagccga tgatcccgat
     8281 ccgcgtacat ctccgtgata tccgcccaga gccgctgaag tacggtgtcc cgagagtatt
     8341 ctttgagttc cttttcgtgg tgggcgttga tcaccacata cagtcccggc aaagatagcg
     8401 catagtcgat gaccttggtg atctccgcaa ggcgtggatg ctgatggtcg acatcgccga
     8461 cgagcgggtc atggaccaat cgtgtgccat tgaccttctc ggtccacgtg atcggaatac
     8521 gcaaattgcg aaaacccatg gcatagtagg cgtcaatttt tttcttggcg acctcgaagg
     8581 tgcgaggagt ctctttcgta ctttcaaaaa actctccgag attgaagcca taccccattt
     8641 cggcggccgc acgtttggcc gtgagcggtt cctcgggggg ggcgtctgcc ggtgtatccg
     8701 gctgcgcatc gaccaagcct ccctccgtgc cttctcctcc cgctccaacg tctttgccgc
     8761 cgcctccctc cgtcttcgcg tccacgtcaa cacctgtgtc accaccactg ctcacgtcag
     8821 ctccaccatc ttgcccgccc gctcccccct cggccgctgg tttgggcgat tcgacaggcc
     8881 cgtcggatga ttccgaacac gccaatacgg tgagtccact gagtagcgtc agaagagtga
     8941 atacagcttt ggttcgcata cgggtatggt aaaagccaac gcctttccac gtcaagacga
     9001 gcctgcgcag ccgacgacaa tgtcgagtat ctttgccgtg gcaaaaatag gttcagaaac
     9061 agcttgatcg acaatttacc cagccagcct ggctgcggcg cgttgtctaa tatattgaaa
     9121 ataaagacaa agttaatagg tcgaggcgcg ggatgctccc acgaaagcgc gattgacgaa
     9181 gcatctggcc gcgtagtatg atgatcgttt cgctaaccat gagtccatgg gctcgccttc
     9241 tgggaaaaga tgaacatgaa aatgaatcac gcggggatcg ggctgatagc tggtcttggc
     9301 ttggcgcttc tttttgttgg ctgcagtgat gacgaggaaa gcgtcgacgg aacccaaaat
     9361 cccgctggcg gcgggggatc gggcggcacc gcggtggatg cgagtgcgga aggagccgct
     9421 cccgacgctg gtgtcgccgg aaccgggggt aagccaacga gcgacggcgg tgggcccgag
     9481 ggtggtgagc cagatagcgg cgagccagag gccggcaacg gtggagcggg gggcgctgcg
     9541 ccggcgaatt tcatcccgag caaccgcctg aattgcgacg ggcttaccga cggcgaagcg
     9601 gctgtaggca ttagctgggg aaatcccgga aaaggtgaga acgcggacac gggaacctat
     9661 ggcgacacga gcatcaagct gccgggacgc acctcgagtt gcaaactcgc tatcaaaaag
     9721 ggctcgacgg gtcgtccggg cgacggcgct gatagcaccg aaggggattt cggctttttt
     9781 atgggccttt cccccgattg gaaaaccctc gtcggagaag gggaagagct ttgggtgggt
     9841 gtgcggatcc gttttccgtc gagttttagt ttttatacca ataccggctt tctgaaattc
     9901 atccgtctcg aatacgtgca acccgacccc aatcatccaa cgtatggacg agtcgattgg
     9961 ttgatcgaaa acggccttgg agacgcgcag attggttggc gttacggtga tgagaaccag
    10021 gactttggcc ttgacaagct gggaggagag cttttcgtgc tcgacgagtg gaactgggca
    10081 gaggtgtact tcaaggcttc ggccgacccc gccaagtccg tcacgcggtt atgggtcaat
    10141 ggcttcatgc aatatgaaat caccgaaggc aagaatgtcc gaaatcgaac gtcggcgggc
    10201 acgtacgaga catcaaccac gaaaaagatg ctccccacct tgcctgccgc aacgactgcg
    10261 ctggagagcg tcatgctctt cacgtattgg aacgggggtg cgccgaaaac acagtcttgc
    10321 aacgtccaag acgtgattat tcacgataac ggagcggacc ttgcggcgaa ggatgagttt
    10381 ggcaacaaga tgatcggtcc ggatgctttt taagcgagaa gcttgtataa catattgaaa
    10441 ataaagataa atatgcctgt tccccagcgc atggggaacc tgataggttt cctcattttt
    10501 cctgaattgg aaagggatac gagctgagga tttttggatc ttgattttgg cttggtcctt
    10561 tagcccccct tggggcaccc cgagcaggtg ccctgggcta ttgaacgttg gactggcgca
    10621 ctgcggtgaa caatggggga tgaccgtgac cgtttcgcgt ctgccgtgac cgtgaaggaa
    10681 aggccctgga aaaaagaggg gatggttttg aaggggaagg cgcaggggct gtgtgacgaa
    10741 tggggttaac cgcttgtcag gggcgaccgg aggccacaac caggtcatcg atcaaaaacg
    10801 actgcttgat aggcgaccca tctccgatcc acggaccgaa caggagttgg ttcagcttca
    10861 tatctgcatg ttgtgccgtt cgcaggatgg cattggtgcg ttcgaagacc attgcgccat
    10921 tgacccaaac gcgtaagatt ccgtcgggct gtccacgccc tgcgctgatg ctattgagct
    10981 tggcgaagta ttccacgtgg ttccagtcat ttttgttttg tggggtcaat acggtgggtg
    11041 atgccagagt gacaccattg acgtagacgc cgctgccggc gttataacaa gagccggagt
    11101 agcccgaagg caggccgttg cagccggcca ccgcgcggtt ttcggtctcg gcgatgaggt
    11161 cctcgccgcg tcggctttga tcgatgtttt tgccgtcctg gaatcccacc cacagggccc
    11221 ctgccgaagc cgattcccag tggctttcca ggtatagtgt gagatgggtg aacgccggtc
    11281 cttcgtattt atcgtctttg ttggtcaaga agtagaactc atgggggtgg tagggcttgc
    11341 ctgagccgac gaaatcggag ctatatttta cccaatagcc aaggtagacg gcatcggttt
    11401 cggggaatag gtgccgacca gcgccctgac cgctacaatc gggctgacct ttgttgtagg
    11461 tacattcgag ggagtgagac ccggagtggc tgtccgagga gagggttttc gggagtgatt
    11521 cgtaccagcc tcgcgttttg tactccgcgt cttcgaattc ttcggtgaag taaatcgtga
    11581 cttcggggct ggcgcctgcc gcgcctccgc tgccagcccc tgccgcgcct ccgccaccac
    11641 catccggcgc ggtgccggcc tcaccaccgt tgccgctatc tgtctcgccg tccgttgtcg
    11701 tgccgctatc cgtggctggc ttgccaccac ttccagccgc tccggcttct ggcatggttc
    11761 cgtcagcgtt agcatcagcc cccgcgccgc ccgagccccc gttgccggcg gggtttttgg
    11821 gcccgtcggt gctttcatcg tcgccgttgc tgcagccagc aaggaaaaac gccaaggtta
    11881 gtcctgcgat cagcccggtt tcgacatagt tatttctcat attttacctt tttccagaac
    11941 caatgggtgc ggctttgctg ctgccctcga gattagcgtg ggtatatgcc tctgtcgact
    12001 ccatgagcag gcgcgcaaca ccagcgaagg agaaattttc ttgcatgcgt cgacagtgtg
    12061 cgtcgagccg gtttcccccg tttccatttt ttgttgaaat acagtgtttc gcagggatac
    12121 ctcgcagccg aacccgcgaa acgcagagtg ttttccgtga ttcacccttc gaaaacagcg
    12181 cgcgtttgtt ttgacgatat gctatgcttg ctgttctttt ggcaatcgaa tccctctgac
    12241 cgtttgaagc gatgacccat ggacacgata cacatagcga gtcgaccttg tttcgcgatg
    12301 tcttcttgct tgatgctgtc gtgttgggtg tatcggcagc gggagcttgt cggcttgggg
    12361 aatctggaac ctggcgcagt tcaccgagcg aaacggagca gcatccggtc caatgaggta
    12421 aatcaggttt ggaggttcaa tccatgaatc gaatgggttg gttgttggta cttgtcgttg
    12481 ccacggtccc aaccatcggt tcgtgccgtc tcggaccgtc cttggatgac tatcgttcgg
    12541 gaagcggtgg cggtcccggg gtaggagggg ccgcgggagc tagtggcgga gccagtggca
    12601 gcgagggggg agccggtgga attggcggcg ctagcggtgg cgagggcgga gttggtggaa
    12661 ttggcggggc tagcggtggc gagggcggag tcggtggaat tggcggagct gccggtggcg
    12721 agggtggagc cagtggggtt ggggggagcg gtggagcagg agggatagct tacggtcctt
    12781 cgtgcgaagg gcttgaagag atatgtggtc ctgccgggca aaaacaaaat tgctgtgaaa
    12841 acgcgctcgt cgtcggcggt tcgtttccca tgggacgctg tgaagatcca agtgaatcct
    12901 gcacggattc ttttgaaaag ggagatccga atgaactgcc ggaacatacg gccacggtgg
    12961 cgagtttcgt gttggatcga ttcgaagtga ccgtaggccg atttcgtcgt tttctggaag
    13021 cttatcccat gagcaagccc agctcttccg cgggggcgca cccgttgata ccagaaacgg
    13081 ggtggaggag tgcttgggat tccaaactac cagcggataa agataagttg atggtggcgt
    13141 tgaactgcaa tgccgccacc aaaacatgga ccgacagtcc tggttcgttt gaagcgatgc
    13201 caatcaattg tgtcgattgg tatctggcct tcgcgttttg tgcttgggat ggcggacgct
    13261 tgcccacgga ggcggaatgg gaatatgcgg cggcaggagg cgaggagaat cgtcgctatc
    13321 cctggggagc gacggcaccg gacgcgaact ccgcaaatta cgttgccagt gatgacaagc
    13381 ccttcgtggt ggtgggtagt tatccggacg ggatgggtcg ttgggggcat tacgatatgg
    13441 ctggaagcgt gtgggagtgg gttttcgatt actatggtac cgcgtggtat ggcgaaggcg
    13501 gcctaaactg cgacaattgc gcgaatctga cgtcttcctg gtcaggccat cgaatacgtc
    13561 gtggtggcgg gtacaagagt gcagccgggg agcttcgcgc tgtaagccgg gcctccgaca
    13621 ttggagccag ccctcgttat gggttgcggt gtgccagaag tccgtgaacg gagcgacgtc
    13681 aaatcatggg tgctgcgatg ctgcgtcatc tcctggtttt tccccgtgca tttccttcgc
    13741 cctcgactgc cggaaaggtg tgccctccat cgctcggcca caggaccgcc gcagtcacgg
    13801 tcacggcgca cggcccacaa aacggtcacg gtcatcccac tttgatcccc cgcagtgcgt
    13861 tagtccaatg ttttacggca catcttgcca tcgtgccacg atgaggcgca tgaccaaagc
    13921 cttcaaggca aacccgtccg ggaggctgga gactacgctc gacgcatcgt aagatgccga
    13981 cgcaccgatg gcatcccggt ttcctgcgcc cataccacgc agcacgcgct tgactatctg
    14041 aggaacaccc tcccagccga gggcgaaatc cgtgtagcac attgatatta ttgatgaatt
    14101 atagtatccc acatcttgtc tggaccggac ggagccaggt ggtcctggga atgtcggatt
    14161 ggtttggggt tggaaacgtt aacgacgcgc cgtgttgcag caaggattag aattatgatg
    14221 ttgaaattca gtggaatttg agctaatctg aaatgaatct tcaattttct gttgggaggc
    14281 aatatgagac ttcgcgtgat ttcggttgcc ttggtggtgg gctgggcggt tcatgctttg
    14341 ggctgttctt cggaagactc ggaatacgat ggcgg
//
LOCUS       DAOSOR010000068        14364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4242_length_14364_cov_4.520791, whole genome shotgun sequence.
ACCESSION   DAOSOR010000068 DAOSOR010000000
VERSION     DAOSOR010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 14364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14364
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4242_length_14364_cov_4.520791"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="PK710_05580"
     CDS             <1..735
                     /locus_tag="PK710_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT09220.1"
                     /translation="GVIAIQGLQKAFREPVLRGIDLTVPKGCLYGLIGPGAAGKSVLL
                     KHVVGLMRPDKGKIFVDGHDLLQMSELELQGFRKRFGIAFQNNALFDHLSVGENIAFP
                     LRRLHKDMTEQEIQDRVDERLRAVALPGMQDRSPPSLSGGQKRRVGVARATVARPPIL
                     IYDEPAAGLDPVSSQKIFDLLKKEKGDATVIMVSSDLDRLLTVTDRVGMMLDGKIIFD
                     GTTEQAQNSNNEYVRQFVHGLTEGPL"
     gene            732..1532
                     /locus_tag="PK710_05585"
     CDS             732..1532
                     /locus_tag="PK710_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT09221.1"
                     /translation="MSLASPHAAQESLTFRIGKNLFDMSEVAGGMAVLLVRVLWRIAR
                     ANFDWNEFKTNMYKMGVKSTPIVVVTALFVGAIAVIQAAPMVQQYGAYGLLGWGAGFS
                     VLRELGPLLTALMISGRVGANNTAELGTMVVTEQIDALRILAIDPLGFLIAPRLLAIV
                     LTLVIANIYADALALFGAAIASKAMLGVEYGVFLNGLTSGLLNFGDVAHGLIKSAFFG
                     LMMAIASCYFGLAVKGGAPGVGRAVNAGVVASATGIFILDYFVSFIIG"
     gene            1535..2362
                     /locus_tag="PK710_05590"
     CDS             1535..2362
                     /locus_tag="PK710_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT09222.1"
                     /translation="MQVTKVSPNSPASRIETSLAELGDSSIDLLRSARDMYSVFVRTL
                     YYVVRGKREPGAIVQQMFEIGNKSVFFVTVVMGFIGLIGIYQTGVQAQRLLPDFSRMG
                     ATYLQLLIREIAPSVGAMIIATRVGAGIAAEIGSMVVTEQVDALRMCAADPIDFLIKP
                     RFIASIVMTTCLIIWITTVMFCAGAVTAKSFFDVPFRTFFDVSMVQTGDVVICLTKCV
                     AYGAAIPVVSGYCGLSTFGGSEGVGWATTRAVVNSSLAVVFLDLIISVAGFFIFFAR"
     gene            2404..3102
                     /locus_tag="PK710_05595"
     CDS             2404..3102
                     /locus_tag="PK710_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT09223.1"
                     /translation="MLQGVSFDVHDGEVFFIIGASGVGKSVLIKHLVGLLYPDQGEIW
                     LDGQEISRFDELQMMAVRRKCAMVFQHSTLFDSMTCAQNVALPLRKHKNLKGRSAEHE
                     AIKLLEKVHMEAFADRFPAELGDGMRKRVAIARALTLDPQYILFDEPTTSLDPVNARR
                     VDKLIRELSDTLGVTSIVVSHDLVSIFGIADRIVMLYKGQVRLLGTREEFRNTTDGVV
                     EQFTNGYAEGPMDL"
     gene            3113..4138
                     /locus_tag="PK710_05600"
     CDS             3113..4138
                     /locus_tag="PK710_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOT09224.1"
                     /translation="MANPRSIEVKVGLLILIAIGILTGFIVLMGGMSFQPTYRIFVDF
                     DNPGGIQSGSPVRIAGITVGKIKEIQFRGGVEPQANEQRPALVRMTLAIETRHQEAVR
                     ENAVFYVTSQGVLGEQFLAIDPGTRDRPALAENAIVRGLDPPRLDLLLAEGYELLHTT
                     ISALRDNRAQIEETFKGLHSTLTSTGEFFDKNRDRLNRIAENVETITIETNELVRAAR
                     TRYVDDPQVNRIINNIDHASSAIARDSEPLLRDARTTMANVNRLSSTVGSPEEQRKIK
                     QTIQDVSEIASRAKAAVADAQAVVAHIRTGKGTMGALVMDEQLFDDLQEMARDLKHNP
                     WKFFWRE"
     gene            4394..5152
                     /locus_tag="PK710_05605"
     CDS             4394..5152
                     /locus_tag="PK710_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09225.1"
                     /translation="MSEEQKESSVLFSLNELFSLEQERIKQEEEAKVRAVEEAKRAEE
                     DARRRAIEEEQRRLRDEEERKRTEEQRKREEAARLEALRQAEIEKARVTAEEHARLEA
                     MRQQQAHAQELAKISQDKSKKTLMYAVIAAVAFLLIGGTTGGVLWYRADQKAKEEARQ
                     AQLLANQKAEEMAQLQKKLDEQSRKVSDLMDKLSNAEDEATKAKLKAELAAAQKEQNA
                     TRGALQRGSPSDTKPSTPRPACTCVEGDPMCSCL"
     gene            5166..5879
                     /gene="pyrF"
                     /locus_tag="PK710_05610"
     CDS             5166..5879
                     /gene="pyrF"
                     /locus_tag="PK710_05610"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOT09226.1"
                     /translation="MPNHHLAFALDFPSLADARPYFTLSKHIRFAKIGLELFCREGPT
                     VVQQVADAGFHVFLDLKLHDIPATVERAVDNLATLGIHLLTVHTFGGPTMLSRAVQAA
                     QKSGTTQIVGVTVLTSLDTTDLSCLGIDRAVADQTERLAKMAWQAGVRAFVCSPKEVA
                     HLRSTLGSDALLITPGIRPAAAATQDQKRVATPASAIADGADILVVGRPIRDAQDPEA
                     ATLNILAQINQSLASKTIP"
     gene            5876..6631
                     /locus_tag="PK710_05615"
     CDS             5876..6631
                     /locus_tag="PK710_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOT09227.1"
                     /translation="MSRIVIGVQPVREAIRVHGNAIERVLLASASHKDSRPLDGIFRF
                     CQSRDIGVERVDRQTLDRITHGASHQGVLAYAPELQLLDLAELMAPSPTLITALDRIT
                     DPQNFGAIIRSAVAFDSHGILWPEHDSAPLTPATFRASAGAIEHARLCRVSSLPAALH
                     ALHNESMVVVGLAGESPVSLDSLPLHQPTVLVVGAEGSGLRKGVRDACDHIARLPTSH
                     VYDTLNASVSAAIALYEVRRQRLAHDPASQIYL"
     gene            6603..7757
                     /locus_tag="PK710_05620"
     CDS             6603..7757
                     /locus_tag="PK710_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09228.1"
                     /translation="MIPHRKFTFDFWALTLVFAALPLRFALAVRTCLSPDEAYYLAAH
                     RLGFTITDHPPLTTWLVGLADNLDFLSLELRIRLPALVVGTFIPLLLLWISKTLHDQP
                     SHQRWTALLGAFLPLPLAGGFLITPDTPLLLAVAVLLVIHHQTPSWKKNVGLIFACAW
                     GLAAKVSMLVPGLLLLFLSPHRSERLAVAMGMAIALPIALPSLGFQWHHAFVDQSSAV
                     GTLTASAAALGAQLLLWTPAVLWFGFREAWPRPFERGLMLSMSVLLLASALLRQVPPE
                     PNWFAVAALFCIPFAAGPLARGARWVQATTLLLGPAAALLFASHVLWPWLPIPTPVDP
                     SARLHGWRDDNPVAQAPGIGVYGPAAEACIYQKQCEKIAYIFNNYEQFFK"
     gene            complement(7953..9071)
                     /locus_tag="PK710_05625"
     CDS             complement(7953..9071)
                     /locus_tag="PK710_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOT09229.1"
                     /translation="MNSIYLDWNATTPPLPEVVEAVAQASRQWWGNPSSSHGRGAMAR
                     QQVELARERVGQLVGRPAFDVIFTGGGTESNNLAIRGLLDRVGPVICSRMEHPSVLHV
                     VEALQNERTVHWLDVDQRGVVDPEQVRETIVSSSKPCLVVIQWVNHETGVIQPVEEIV
                     QQAHEHGSIVHVDAVQGIGKLQGRPWSEADTISLSSHKIRGPQGIGALVGASCQRLRP
                     MMLGGPQERKLRPGTVPVALAVGFGVAASWASSSPSRYDAIRPLRDHLEREMIQMGAK
                     RNGQGKRVPHVTNVSFEGVLGDELVAACDVEGVCLSAGSACASASSGATSAVAALLGP
                     KRGLEAVRASLGELTTAQEIEKAVEVIRRVVGRASGDG"
     gene            complement(9068..10279)
                     /locus_tag="PK710_05630"
     CDS             complement(9068..10279)
                     /locus_tag="PK710_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOT09230.1"
                     /translation="MAVPRVLHIEDDPANRLLVRKILVGAGFEVVDAADGLEGVRIAR
                     EIQPDLVLVDLNIPGLDGYEVTLRLRGDPGLREVPIVAITAEGDRSTSLAVGCDGFVQ
                     KPINVRTFAQTLEKFLRGGKEVVSAAKADMHLRAQSQRIVARLEAKVAELSEANDKLR
                     EAERARTTFYRNVSHELATPMTPIVGYVRLLVDEELGALNPAQRKALQAMDGCVQRLR
                     ALIDNLLDVTGLEAGTMRFVHRDYDVRSVVERELGRFQGLAAQGSVRLVFECAELKMP
                     AWGDAERLGRGIAQLIDNALKFTQPGGVVGVRLQHTGSFYEVCISDNGPGIPVSHIDK
                     VMEPFVQADGSPTRKHQGVGVGLAIARKIARGLGGELLVESPTHERIGGMVFRGTSCK
                     LSVAQRAPQPS"
     gene            10371..10883
                     /locus_tag="PK710_05635"
     CDS             10371..10883
                     /locus_tag="PK710_05635"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT09231.1"
                     /translation="MVPSRMPFLGAYLLFAACSNLTSPAGENPDRLSPVSDSSAPTRT
                     PPVAALPSALRPTLEAQPTRMTVRRIVIAHDGTEGIKPKAPRTLAEARKLASDIAQQV
                     KAPDADFSKLAQMHSDAPDAQRGGLLAPIRADSDIDPALRTAIQQAKVGDISNVIETR
                     EGLQIIQRLR"
     gene            10892..11263
                     /locus_tag="PK710_05640"
     CDS             10892..11263
                     /locus_tag="PK710_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT09232.1"
                     /translation="MAKKRILIVEDDPSVRTLLEKALALDYEIESVADGVAAVRRISQ
                     PPAADVILCDIMLPGVDGFEVARRAKASSHWSNVPIVFLTARTTPKDVILGIQAGARH
                     YLTKPFKIQEVLDKLARITRG"
     gene            11275..12849
                     /gene="lnt"
                     /locus_tag="PK710_05645"
     CDS             11275..12849
                     /gene="lnt"
                     /locus_tag="PK710_05645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOT09233.1"
                     /translation="MTPTRVDSSLERPLPLRLTLPLCILAGIFYFASFPGIGLWPLGF
                     VALVPLLISLRNQSIVVAALLGLACGTTATSLGFSWLIDTFRHHGGFGTLTAAVLLLI
                     LSVLQAGRFALFAALVSGLHRRAWPWTACVAGAFVTSETIYPLIFPWYFGAVVHDIPF
                     LLQTADLGGPVLVGLPLVLVNAACADVFRASKPQFPSKSAWGTAALMLAGTVIYGAFL
                     IVWTDHRTQSAPLFRVGLVQGNVPMPPASSEQLAQKFRTQVELSRRLIAEGAQLVVWP
                     ESAFLHVLASSDPASLVARTEAATLEAPLLTGAILESHDGKLFNSALLLGPDGQLRGR
                     YDKQHRLAFGEYLPFGETFPILSRWSPQSGSISKGIASGPLAFESRRISVLLCYEDIL
                     PSYVRDVVQTHRPHLLVNLTNDGWFGQSRAAVIHLALAKLRAVEHRRYLLRATNTAMS
                     AVIDPVGRQQAVLEPFIENTYIGDLRWLHGWTLYGFWGEFPWYCVAIGTLGGCMLRRG
                     GRGLHSFWDKKRVNSE"
     gene            13022..13624
                     /locus_tag="PK710_05650"
     CDS             13022..13624
                     /locus_tag="PK710_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09234.1"
                     /translation="MKKLALHWTLVASALALAFAACTVTTEDDENNGGSGGDAGSGGT
                     ETGGTGGGDTGGTGGGATGGTGGGATGGTGGGVTGGTGGGVTGGTGGGVTGGTGGGET
                     GGTGGGSSTKTCEELYPEADSCSTCMRTNCCKEVEACFNEESQECRKSWDCLAEKCTD
                     PNTFEECKVQCDDGNFNVAYNEITTCLEQKCFEGCGTAEE"
     gene            complement(13729..>14364)
                     /locus_tag="PK710_05655"
     CDS             complement(13729..>14364)
                     /locus_tag="PK710_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOT09235.1"
                     /translation="CAGFPVPATRYFREFLFVEVQETHLGAPGPGTVRRWRREAPEGF
                     RFALLGPREIGQEGFREGKVVETAMKSLVAVAAELSAEVAVFVAPPDFPCNRANKTVV
                     KDFLASTRKMFKLVVFDPGPLWEADETESLLTADQVLVVRDPLNQGTSKKKRAYYRLP
                     GPAGHKSRYEDPAIERLAEIAKAAKHTEATYVFTNVDMFADAKRFRKAMKL"
BASE COUNT         3083 a         4585 c         3582 g         3114 t
ORIGIN      
        1 ggtgtgatcg caatccaagg cttgcaaaag gcgtttcgtg agcctgtcct tcgcggtatc
       61 gacctgaccg tccccaaagg ctgcctgtat ggtctcattg gccctggcgc tgccggaaaa
      121 agcgttttgc tcaaacacgt cgtgggattg atgcgacccg acaaaggaaa aatctttgtc
      181 gatggtcacg accttctcca aatgtccgaa ctagagctgc aaggcttccg aaaacgcttc
      241 ggtatcgctt tccaaaacaa tgccctgttc gaccacctgt ctgtcggcga aaatattgcc
      301 ttccccttgc gacgccttca caaggatatg acggaacaag agattcaaga ccgtgtcgac
      361 gaacgactgc gagctgtcgc cttgcctggc atgcaggacc gcagccctcc cagcctctcc
      421 ggcggacaaa aacgacgcgt cggagtggct cgtgccacgg tcgctcgacc acccatcctc
      481 atttacgacg agccagccgc gggactcgac cctgtcagct cccaaaaaat attcgacctg
      541 ctcaaaaaag aaaaaggaga cgctaccgtc atcatggttt cgtcggacct cgaccgattg
      601 ctgacggtca ccgatcgggt tgggatgatg cttgacggca agatcatctt cgacggaacc
      661 acggagcaag cgcaaaacag caacaatgag tacgttcgac aattcgtcca cggattgacg
      721 gagggccccc tttgagtctt gcatctcctc acgctgccca agagtccctt acctttcgaa
      781 ttggcaaaaa cctattcgat atgtccgagg ttgcgggtgg catggccgtg cttctcgtcc
      841 gcgtgctttg gcgcatcgcc cgtgcaaact tcgattggaa cgaattcaaa acgaatatgt
      901 ataaaatggg tgtcaaatcg actcccatcg tggtcgtcac ggcgcttttc gtgggagcca
      961 tcgcggtcat tcaagcggct cccatggtgc aacaatatgg ggcctacggt ctgctcggat
     1021 ggggagcggg gttctccgtc ttgcgcgagt tgggcccctt gctcaccgcg ctcatgatct
     1081 ccggacgagt cggcgcaaat aacaccgccg aattgggaac catggtcgtc accgaacaga
     1141 tcgacgcgct gcgcatcctt gccatcgatc cccttggatt cctcatcgcg cctcggctcc
     1201 tcgccatcgt acttaccctt gtcattgcca atatttacgc cgatgccctc gctttatttg
     1261 gcgctgccat cgctagcaaa gccatgctcg gcgtcgaata tggcgtattt ctcaacggcc
     1321 ttaccagcgg tctgctcaac ttcggcgatg tcgcccatgg cttgattaaa agcgcgttct
     1381 ttggactgat gatggcaatc gcctcgtgtt attttggcct cgcggtaaaa ggaggagccc
     1441 caggagtcgg tcgagccgtc aatgccggcg tcgttgcttc cgccaccggt atcttcatcc
     1501 tcgattactt cgtttccttc atcatcggtt gaacatgcaa gtcaccaagg tatcgcccaa
     1561 ctcccccgcg tcacgtatcg agacatcact cgctgagcta ggcgattcct ctatcgatct
     1621 gcttcggtcc gcccgcgata tgtactcggt tttcgttcgc accttgtact atgtcgttcg
     1681 cggaaaacgg gaaccgggcg ccatcgtcca gcagatgttc gagatcggaa ataaatccgt
     1741 cttcttcgtc actgtcgtca tgggcttcat cggcctcatc ggcatttatc aaaccggcgt
     1801 ccaagcacaa cgcctccttc ccgacttctc ccgcatggga gccacgtatt tgcaacttct
     1861 cattcgagaa atcgctccct ccgtcggcgc catgatcatt gcgactcgcg tgggggccgg
     1921 catcgccgcc gaaatcggtt ccatggtcgt caccgaacaa gtcgacgcgc tgcgcatgtg
     1981 cgccgccgac cccatcgact ttctcatcaa accaagattc atcgccagca tcgtcatgac
     2041 cacctgcctc atcatctgga tcaccacggt catgttctgt gccggcgctg tcaccgcaaa
     2101 atcattcttc gacgttccct tccgtacttt cttcgacgtc agcatggtgc aaaccggcga
     2161 tgtcgtcatt tgtctgacca agtgcgtcgc ttatggcgct gccattcccg tcgtttcggg
     2221 atattgtggt ctgtccacgt tcggagggtc cgaaggcgtc ggatgggcaa ccacccgcgc
     2281 ggtcgtcaac tcttcccttg cggtcgtttt tctcgacttg atcattagcg tcgcaggctt
     2341 tttcatcttt tttgcacgat gatttccttt cgaaacatcc ataagagctt cggacctaaa
     2401 aatgtgctcc agggtgtttc tttcgacgtc catgatggcg aggtcttctt catcatcggt
     2461 gcctccggcg ttggaaaaag cgtgctcatc aaacacctcg tcggcttgct ctatcccgac
     2521 cagggggaga tctggctcga tgggcaggaa atctcgcgtt tcgacgaact tcagatgatg
     2581 gccgtacgtc gaaaatgcgc gatggtcttt cagcattcga cgcttttcga ttcaatgact
     2641 tgcgcacaaa acgtggcgct tccgttacga aaacacaaaa atctgaaagg ccgtagtgcg
     2701 gaacacgaag cgatcaaact gctcgaaaag gtacatatgg aagcgttcgc ggaccgcttc
     2761 ccggcggagt tgggcgatgg catgcgaaaa cgcgtggcca tcgcacgcgc cctgactctg
     2821 gacccccagt atattttatt cgatgaacca acgaccagcc tcgatcccgt caacgcacgc
     2881 cgcgtggaca agctcattcg cgaactgagt gataccctcg gagtcaccag tatcgtcgtc
     2941 tctcacgacc tcgtctcgat ctttggtatc gccgacagga tcgtgatgct gtacaagggg
     3001 caagttcggc tccttggcac ccgggaggaa ttccgaaaca ccaccgacgg cgtcgtcgaa
     3061 cagttcacga acggctacgc tgaaggcccc atggacttat aatgagggga gcatggcgaa
     3121 cccgcgttcc atcgaagtca aagttggtct cctgatcctc atcgcaattg gaatactgac
     3181 tggcttcatc gtgctcatgg gaggaatgag cttccaacct acctaccgga tcttcgtcga
     3241 cttcgacaat ccagggggca tccaatccgg ttctcccgtg cgcatcgccg ggattaccgt
     3301 cggaaaaatc aaagaaatcc aattccgtgg aggcgtcgaa ccccaggcga acgaacagcg
     3361 tccagccctc gtgcgcatga cgttggccat cgaaacacgc catcaagaag cggttcgaga
     3421 aaacgccgtc ttttatgtca cttcgcaagg cgtgctgggt gaacaattcc tcgcgatcga
     3481 tccaggaacc agagaccgac ccgccctcgc ggaaaatgcc atcgtgcgag gacttgatcc
     3541 ccctcgtctc gatctattgc tcgcggaagg ttatgaattg cttcacacca ccatctccgc
     3601 attgcgagac aatcgtgcgc aaatcgaaga aaccttcaaa ggcctgcaca gtacattgac
     3661 cagtaccggt gagttcttcg acaaaaatcg cgaccgactc aatcgtatcg ccgaaaacgt
     3721 cgaaaccatc accatcgaaa ccaatgaact cgttcgcgct gcacgcaccc gatacgtcga
     3781 tgaccctcaa gtcaaccgca tcatcaacaa catcgaccac gcctcctccg ccatcgcacg
     3841 ggatagcgaa ccactgctgc gcgatgcgcg aaccaccatg gcgaacgtca accgcctgtc
     3901 ttccacggtc ggctcccctg aagaacagcg gaaaatcaag caaaccatcc aagacgtatc
     3961 ggaaattgcc agtcgcgcca aagccgccgt agccgacgcc caagctgtcg tcgcccatat
     4021 tcgcaccggc aaaggtacca tgggcgccct ggtcatggac gagcagctat tcgacgattt
     4081 gcaagagatg gcccgggacc tcaagcataa tccatggaaa ttcttctggc gtgaataacc
     4141 caagcccaat ggttccgcac ttcattcgca cgggcaacga cccttctctt gcccttggcc
     4201 ccactacggg ctacgatcca cttattcttt tcgtggatcg accacagacc cgtagcaggc
     4261 tccttcgagc cactgtcaaa ggaaccgtcg gtctccgatt gacgtctcct ttccagattg
     4321 ttagtgacgg taccgtgtct cgatgacacg tcacccctat ccgcaagcga gcgctgtgag
     4381 gtagcaccac accatgagcg aagagcaaaa ggagagctcg gttcttttct ccctcaacga
     4441 gctgttcagc ctcgagcaag agcgaatcaa gcaggaagag gaagccaagg tcagagccgt
     4501 cgaagaggcg aaacgggccg aagaagacgc tcgcaggcgc gccatcgagg aagagcagcg
     4561 gcgccttcgc gacgaagagg aacgcaaacg aaccgaggaa cagcgcaagc gtgaagaagc
     4621 agcacgcctc gaagccctac gacaagccga aatcgaaaag gctcgcgtca cggctgaaga
     4681 gcatgctcgc ctcgaagcca tgcggcaaca gcaagctcac gcgcaagagc ttgccaaaat
     4741 tagtcaagac aaaagcaaaa agaccttgat gtacgcggtc attgcagcag tagccttcct
     4801 gctcatcggt ggtaccaccg gtggagtcct ctggtatcgc gccgaccaaa aagccaagga
     4861 agaggcccgt caggctcaac tgctcgccaa tcaaaaagcc gaggaaatgg cccaactgca
     4921 aaagaaactc gacgaacaaa gccgcaaggt cagcgatttg atggataaac tatcgaacgc
     4981 ggaagacgag gccaccaaag ccaaactaaa ggctgagttg gctgccgcac aaaaagagca
     5041 gaatgctaca cgaggcgctt tgcaacgcgg ctcgccgtcc gacaccaaac cctccacgcc
     5101 tcgtcccgct tgcacatgcg tcgaaggcga cccgatgtgt agctgcctgt agcctctaca
     5161 tcaacgtgcc caaccaccac ctcgccttcg ccctcgactt cccatccctc gcggatgctc
     5221 gaccctattt cacactcagc aaacacattc gcttcgccaa aatcggcctc gaattgtttt
     5281 gccgtgaagg ccccaccgtc gttcagcaag tcgccgacgc cggattccac gtgttcctcg
     5341 acctcaaact ccacgatatc cccgccaccg tggaacgcgc ggtcgataac ttggctaccc
     5401 tcggcatcca cctgctcacc gtccatacct tcggcggccc caccatgctc tcccgagcgg
     5461 tccaggccgc ccaaaaatcc ggaaccactc agatcgtcgg ggtcactgtc ctcacttccc
     5521 tcgataccac cgacctgtct tgcctcggta tcgatcgggc cgtcgcggat caaacagagc
     5581 gcttggccaa aatggcctgg caagccggcg ttcgcgcctt cgtctgctcc cccaaagaag
     5641 tcgctcactt gcgatccacc ctcggtagcg atgccttgct catcaccccc ggaattcgcc
     5701 ctgcagccgc tgccacccaa gaccaaaaac gtgtcgccac ccccgcttcc gccatcgccg
     5761 atggcgccga tatcctcgtg gtgggacgac cgatccgcga tgcgcaggat cctgaagccg
     5821 caaccctgaa tattctcgcg caaatcaatc aatccctagc ctcgaagacc atcccgtgag
     5881 ccgcatcgtc atcggcgtgc aacccgtccg agaagccatc cgcgtgcatg gcaacgccat
     5941 cgaacgtgtt ctgctcgcca gcgcatccca caaagactct cgccccctgg acggcatctt
     6001 tcgtttttgc caatccaggg acatcggtgt cgaaagagtc gaccgccaaa ccctcgatcg
     6061 aattacccat ggtgccagtc accaaggagt attggcctac gcgccagaac tccaactgct
     6121 cgacctagcg gaactcatgg cccccagccc aacattgatc acggccctgg accggatcac
     6181 tgatccacaa aactttggcg ccatcattcg aagtgccgtc gcctttgatt cccacgggat
     6241 cctgtggccc gagcacgact ccgctcccct gactcccgcc acattccgcg cctccgccgg
     6301 cgccattgaa cacgctcgac tctgccgcgt ctcttcccta cccgccgcat tacacgccct
     6361 ccacaacgaa agcatggtcg tcgtgggact ggccggcgaa tcccccgttt ccctcgattc
     6421 actgcctcta caccaaccca cggtactcgt ggtgggagca gaaggctcgg gtctccgaaa
     6481 aggagtgcga gacgcttgtg atcacatagc tcgactgccc acatcccacg tctacgacac
     6541 tctcaacgcc tccgtgtccg cggccatcgc tctttatgag gtacgacgcc aacggttggc
     6601 ccatgatccc gcatcgcaaa tttacctttg atttttgggc acttacgcta gttttcgcag
     6661 cgcttcctct ccgctttgcc ctggctgttc gcacctgtct atcccccgat gaagcctatt
     6721 acctcgccgc ccacaggctc ggattcacca tcaccgacca tccgcccctg acgacttggc
     6781 tcgtcggact cgccgacaac ctcgattttc tatctcttga actgcgcatc cgtcttcctg
     6841 ccctcgtggt gggaacgttc atcccgctgc ttctgctctg gatttccaaa acccttcacg
     6901 atcagccaag ccaccaacgc tggacggccc tgctcggtgc ctttttgcct ctgccgctgg
     6961 ctggcggttt tttgatcacg cctgatactc ccctgctgct agccgtcgca gtgctgctgg
     7021 tgatccacca tcagacacct tcttggaaaa aaaacgttgg tttgattttc gcctgcgcat
     7081 ggggcctggc ggcaaaagtc tcgatgctcg ttcctggctt gcttcttctg ttcttgtccc
     7141 cacaccgcag cgaacgcctc gccgtggcaa tgggcatggc gattgccctt cccatcgccc
     7201 tgccctctct tggttttcaa tggcaccatg ctttcgtgga ccaaagctcc gccgtgggaa
     7261 ccttgacggc ttcagccgct gccctgggcg ctcaactcct gctctggact cccgcggtac
     7321 tttggttcgg ttttcgcgaa gcgtggcctc gacctttcga acgcggattg atgctatcga
     7381 tgtccgtgct gctcctggct tcggcactgc ttcgccaagt cccccccgaa cccaattggt
     7441 ttgccgtcgc cgcgctattt tgcattccgt tcgctgccgg tccccttgcc cgtggcgcaa
     7501 gatgggtgca agccacgaca ctgctgcttg gacccgcggc cgccctgctg tttgcatctc
     7561 acgttctatg gccctggctt cccattccga cccccgtcga tccttccgcc cgattgcacg
     7621 gctggcgaga tgacaacccc gtcgcccagg ccccaggaat tggggtgtat ggtcccgccg
     7681 cggaagcctg tatctaccaa aaacaatgtg aaaaaatagc ttatatattc aataattacg
     7741 aacaattttt caagtgatca ttgaagacag gaaccgggtt gcgatttcca gaacacgaac
     7801 tgaacacttg acaagtccat gggtttgtcg gatccattca atagaacgtt ccatcgcgcg
     7861 aagctgaacg taaatacccc ttccatctcg tagagttacg gctggtcttt ccatgaaagg
     7921 atcaaggttt aagaagtact tcaagaagta cttcatccat caccgctagc tcgtccaacc
     7981 acccttcgga tcacctcgac cgccttttcg atctcttgtg ctgtcgtcaa ttcacccaaa
     8041 cttgcccgca ccgcttccag cccacgcttt ggccccaaca acgcagccac cgccgacgtt
     8101 gcccccgagc ttgccgaagc gcaggcactg cctgccgaaa gacaaacgcc ttccacatcg
     8161 caagccgcta ccaactcatc tcccaacaca ccttcgaacg atacattcgt cacatgcggt
     8221 acccgttttc cctgtccatt tctcttggcc cccatctgga tcatttctcg ttcgagatga
     8281 tcccgtaagg gtcgaatcgc atcgtaacgc gacggactcg aactggccca gcttgctgca
     8341 actccaaacc ccaccgcgag tgccaccggc accgttccag gtcggagttt gcgctcctga
     8401 ggcccgccca acatcatggg ccgtaaccgc tggcagctcg cccccaccaa cgccccaatc
     8461 ccttgcggac cacgtatctt atgcgatgac aacgatatcg tatccgcctc ggaccacgga
     8521 cgcccctgaa gctttccaat cccctgcacg gcatccacat gcacaatcga accatgttca
     8581 tgcgcctgct gcacaatctc ttccaccggt tggatcaccc cggtctcgtg attgacccat
     8641 tggatcacca ccaagcatgg tttcgaactc gatacgatcg tttcgcgaac ttgctccgga
     8701 tccaccaccc ctcgctgatc cacgtcgagc caatgcacgg tgcgttcgtt ttgcaatgct
     8761 tccaccacat gcaaaacgga tgggtgctcc atccgcgagc aaatcaccgg ccccacacgg
     8821 tcgagcaaac cacgaatcgc caaattgttg gattccgtcc ctccccccgt aaaaatgaca
     8881 tcgaaagcgg gacgtcccac aagctgtccc acccgctcgc gcgccaattc gacttgctgc
     8941 cgtgccatcg ctccgcgccc atgcgacgag gaagggtttc cccaccattg acgagacgct
     9001 tgcgcaacgg cttccaccac ctcgggcagc ggtggagtcg tggcattcca atccaaatag
     9061 atggaattca cgatggttga ggcgcccgtt gtgccaccga tagtttgcat gacgttccac
     9121 ggaaaaccat gcccccgatt cgttcgtggg tgggagactc cacaagcaat tcccctccca
     9181 atcctctagc tatctttctt gctatagcca atcccacgcc aactccttga tgctttcggg
     9241 tgggtgatcc atccgcttgg acaaacggtt ccatgacctt gtcgatatgg ctcacgggta
     9301 tccccgggcc attatccgaa atgcatactt cataaaaaga acccgtatgc tgcaaacgca
     9361 cccccaccac cccccccggt tgcgtaaatt tcaatgcatt atcaattagt tgcgcgattc
     9421 cccgccccaa gcgctccgcg tcgccccacg ctggcatttt caattcggca cactcgaaca
     9481 caagacgtac ggagccttgt gccgccaatc cttgaaacct gccaagctct cgttccacca
     9541 ccgagcgcac atcgtaatct cgatgcacaa agcgcatcgt ccccgcctcc aagcccgtca
     9601 catcaagcag attgtcaatg agcgcccgaa gacgttgcac acaaccgtcc atcgcttgta
     9661 aggctttgcg ttgcgctggg ttcaacgcac cgagttcctc atcgaccaac agcctgacgt
     9721 aacccacaat cggcgtcatg ggggtggcca gttcgtgaga tacattgcga taaaacgtgg
     9781 ttcgcgcccg ttcggcttcg cgtagcttgt cattggcctc cgacaactcc gccactttcg
     9841 cttccaacct cgccacgatc cgctggcttt gcgcacgcaa atgcatatcg gctttcgcag
     9901 ccgacaccac ttcttttcct ccccgcaaaa acttttccag cgtctgtgcg aaagtccgca
     9961 cgttgatcgg cttctgcacg aagccatcgc atcccaccgc aagactcgtc gaccgatcac
    10021 cttccgcggt aatcgcaacg atcggcactt cccgcagccc tggatcccca cgaagtcgta
    10081 gcgtcacctc atacccatcc aaccccggaa tattgaggtc gacgagcacc aggtcaggtt
    10141 ggatttctcg tgcgattcga accccttcca atccgtcggc cgcatccaca acctcaaacc
    10201 cggcgcctac caggattttc cgcacgagga gccggtttgc ggggtcatct tcgatatgta
    10261 acacacgagg aacagccaca ttcgcacctt ggcgcaaata gggcgctggc tgcaagccta
    10321 gagagcccct gtcccttgcc acccatgagc cctgaaggta tgaagcgccc atggttccca
    10381 gtcgaatgcc atttttgggc gcttatttgc tctttgccgc ctgctcgaat ctcacctccc
    10441 cagcgggcga aaaccccgac cgattgagcc cggtctccga ttcttccgcc ccgacacgaa
    10501 ctccccccgt agcagcctta ccttccgctt tgcgtccaac cctcgaggct cagcccaccc
    10561 gcatgacggt gcgacgtatc gtcattgccc acgacggaac agaaggcatt aaacccaaag
    10621 ctcctcgcac gcttgccgaa gctcgcaaac ttgcttcgga tatcgcgcag caagtgaagg
    10681 ctcccgacgc cgacttttcc aaacttgcac aaatgcattc ggacgcaccc gatgcgcaac
    10741 gcggtggcct gctggctccc attcgtgctg attcggatat cgatccggcg ctgcgcacgg
    10801 ccattcagca agccaaggtg ggagacatct cgaatgtgat cgaaacacgg gagggactgc
    10861 aaatcattca acgcctgcga tgatccaagg tatggccaag aaacgaatac tcattgtgga
    10921 ggatgaccct agcgtacgaa cgctcctcga aaaagcgctc gccctcgact acgagatcga
    10981 atccgtggcc gacggcgtgg cagcagttcg acgcatctcc caacctcctg cggccgacgt
    11041 catcctctgt gacatcatgc tcccgggcgt ggatggattc gaagtagcgc gaagggccaa
    11101 agcgtcttct cattggtcca acgttccgat tgtgtttctt acggctcgta ccacgccgaa
    11161 agacgtgatc cttggaatcc aagccggagc cagacactac ctgaccaagc cgttcaagat
    11221 tcaagaagtg ctcgacaaac tcgcccgcat tacccgaggg taatcgtggg cctcgtgaca
    11281 cccacccgtg tcgattcatc cctcgaacga ccattgccat tacgcttgac gctgcccctt
    11341 tgcattctcg caggcatttt ctattttgct tcgtttccag gtatcggact ctggcccctc
    11401 gggttcgtcg cgctcgttcc cctgctcatc agcctacgaa accagagcat cgtcgtggct
    11461 gctctgctgg gcctggcgtg cggaactacc gcaacttccc taggcttttc ctggcttatc
    11521 gacacctttc gccaccacgg gggcttcggt acgctcaccg cagccgtgct gctgctcatc
    11581 ctaagcgtcc tgcaagcggg tcggtttgcg ttgtttgccg ccctggtcag tggccttcat
    11641 cgtcgcgcct ggccctggac cgcgtgcgtc gctggggcct tcgtcacctc cgaaaccatc
    11701 tatcccctta tttttccatg gtattttgga gccgtggtcc atgatatccc cttcctgttg
    11761 caaaccgcgg acctcggcgg ccccgtactc gtgggtctcc cgctggtgct cgtcaacgct
    11821 gcctgcgccg atgttttccg cgcatcgaag ccacaattcc cttccaagtc agcgtggggc
    11881 accgcggctc tgatgcttgc gggcaccgtg atctatggcg cgttcctgat cgtttggacc
    11941 gatcacagaa cgcaatcggc gcccctattt cgtgtgggat tggttcaggg caacgtaccg
    12001 atgccgccag cctcttcgga acaactggca caaaagtttc gcacccaagt cgaattgagc
    12061 cgtcggctca ttgccgaagg agcgcagttg gtggtttggc cggagtccgc attccttcac
    12121 gtacttgcct cgtcggatcc cgcttccctc gtggcaagaa cggaagcggc aacgctggaa
    12181 gcgccgttgt tgaccggtgc catccttgaa tctcacgatg ggaaactgtt caattccgcg
    12241 ctgttgttgg gacctgatgg tcagttgcgc ggccgatacg acaaacaaca tcgtcttgcc
    12301 tttggcgagt accttccttt tggggagacg tttccaattc tatcccgatg gtccccccaa
    12361 tcgggatcga tatcgaaagg gatcgcatcc ggcccccttg cctttgaaag ccgaagaatc
    12421 tcggtattgt tgtgttacga agacatcctc ccttcctatg tgagggatgt cgtccagaca
    12481 caccgccctc atctgctggt gaacctgacc aacgacgggt ggtttggaca atctcgcgcg
    12541 gcagtcatcc atttggcgtt ggcgaaactg cgagcggtgg agcatcgacg ttatctgctg
    12601 cgggcgacca acacggcgat gagcgcggtt atcgatccgg tggggcgcca gcaagctgtt
    12661 ttagaacctt ttatagaaaa tacatatatt ggagatttgc gatggctcca cggttggaca
    12721 ctctatggtt tttgggggga atttccatgg tattgcgtgg cgatagggac cttggggggg
    12781 tgtatgctgc ggcgaggcgg ccgcggattg cattcatttt gggataagaa aagggtgaat
    12841 agtgaatagg acgactggcg catcgtccat ctgcgagtca gaatcttgac ttgctaaccc
    12901 agaagagact acgtcaacaa gcgatccgtg atgggaatga atttcggttg tcaccgtatg
    12961 attgggatgg aacctaacga cggtgacctt ggaggctgaa ggcttttcaa aggagaatca
    13021 aatgaagaaa ctagctttgc actggacttt ggttgcttcc gcgctcgcgc tggcgtttgc
    13081 tgcctgcacc gtcactacgg aagacgatga aaacaacggc gggtccggcg gagatgctgg
    13141 ctcgggcggc acggaaactg gcggaacggg tggcggcgat accggcggaa cgggcggtgg
    13201 cgcaaccggc ggaacgggcg gtggcgcaac cggcggaacg ggcggtggcg taaccggtgg
    13261 aacgggcggt ggcgtaaccg gtggaacggg cggtggcgta accggtggaa caggcggtgg
    13321 cgagactggc ggaacgggcg gcggatcctc tacgaaaact tgcgaggaat tgtatccaga
    13381 agccgattct tgttccacct gcatgagaac caattgctgc aaggaagtcg aggcctgttt
    13441 caacgaagag tcccaagaat gcagaaaatc atgggattgc cttgctgaga aatgcaccga
    13501 tcccaacaca ttcgaggaat gcaaggtaca gtgcgatgac ggcaacttca atgtagccta
    13561 taatgagatc acgacctgcc tcgaacaaaa gtgcttcgaa ggctgcggca ccgcagaaga
    13621 ataagatttc attttaccaa aaccctgaaa acgccgcctt ttcccaagaa taggcggcgt
    13681 tttcttggct tcccctcttt caatgatcgc cgattcagtg aaccagattc acagcttcat
    13741 ggcctttcga aaacgcttgg catccgcgaa catatccaca ttggtaaaaa cgtacgtggc
    13801 ctcggtatgc ttggctgctt tggcgatttc cgcaagacgt tcaatcgccg ggtcttcgta
    13861 ccttgactta tgccccgccg gtccgggaag acgataatac gctctctttt tctttgaagt
    13921 cccttgattc agagggtctc gcaccaccag cacctgatcg gccgtcaaca agctttcggt
    13981 ctcatcggct tcccacaacg gaccaggatc gaaaaccacc agcttgaaca ttttacgtgt
    14041 ggacgccaag aaatccttga ctacggtctt gtttgcgcgg ttgcaaggga agtccggagg
    14101 cgccacgaat acggcaactt cagcgctcaa ttcggcagca accgccacca aactcttcat
    14161 ggccgtctcc acgacttttc cttcgcgaaa cccctcttgc ccaatttcgc gggggccaag
    14221 cagcgcaaaa cgaaatccct ccggcgcttc ccgtcgccac ctgcgcaccg tacctggccc
    14281 cggtgctcca agatgcgtct cttgcacctc gacaaagaga aactcgcgga agtacctcgt
    14341 ggctggaacg ggaaaccctg caca
//
LOCUS       DAOSOR010000069        14301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4267_length_14301_cov_3.762319, whole genome shotgun sequence.
ACCESSION   DAOSOR010000069 DAOSOR010000000
VERSION     DAOSOR010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 14301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14301
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4267_length_14301_cov_3.762319"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PK710_05660"
     CDS             <1..194
                     /locus_tag="PK710_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09236.1"
                     /translation="VRVRMLEQDVGPRRYRMTYHLVDGAAIPGGAVDRLARLVEAMVR
                     NGEHRAAAYLADRRGGRKK"
     gene            251..709
                     /locus_tag="PK710_05665"
     CDS             251..709
                     /locus_tag="PK710_05665"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT09237.1"
                     /translation="MRRLATVFKALSDETRLAMLGLLLREGELCVCDFVEVLGITQSK
                     ASRHLRYLVHAGLLQDRRELTWVYYRMADQLGAEQRLVLSGVQELVKQQLPDELLESL
                     KTWKQQKQCDGGCGPSPKKRLSSCRGRCEGTIVRPLALFENGIMKRSPRS"
     gene            706..6024
                     /gene="uvrA"
                     /locus_tag="PK710_05670"
     CDS             706..6024
                     /gene="uvrA"
                     /locus_tag="PK710_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOT09238.1"
                     /translation="MNRTVLRNARTHNLQSVCIELEPGQLIAVTGVSGSGKSSLCLDT
                     LYAEGQRRFVESFSPYARQFLERHERPPIDSLDPVPAAIAVDRHAPVKSSRSTVATMS
                     DVEPYLSALFLREGRPICPNCAVGATWMDAGVAASSLVWEWADERVIVLAPGRAEGTA
                     AYLDARDRLVAAGHRRLWLADSIRDIDTVRPSEILSEGSRFEVVIDRIKLGEPSLRRL
                     QEALEQAWTMTQGQAVIVGESKGRRTVQRGLVCPSCAKSFAPPRPGLFSYQSPVGACE
                     ECHGFGRVIGIDFQKVFPDAAKSLAQGAIRPWSGKTSAWERRTLKKFCLRHDIDMDRP
                     WGQLTKKQQQLVITGDGTWSGGKYPGIIAWFRYLESKAYKMHVRVMLSRYRAYDPCPV
                     CRGKRLNAAALSYGVDGLDLAGWHGLELGEARARLMGWKATHPQGELIRKELLSRLGF
                     LCDVGLGYLTLDRQARTLSGGEAQRVSLTAALGASLSGALIVLDEPTVGLHPSDISPL
                     VDCMRSLADHGNTVVVVEHDPSVVRAVDRVVELGPGAGAKGGRILFEGSPEQLTARTD
                     LPTGRALAAWQENQPSNRRKWHKTLRVHNARANNLATIDVDIPLGVICALSGPSGSGK
                     STLAEEIVYRAVARAHGMTDVDPPAEHDRLEGVEHLCGVTLVDQNPLGRTSRGNPATY
                     TKAWDRVRALFASEPAAQGLGFTPSHFSFNVAAGRCDACAGEGFETVEMQFLADISLL
                     CPVCRGKRFKSEVLEVVHHGKTVADLLAMTVDEVVQWANHDPALVRALRPLQSLGLGY
                     LPLGQPLSTLSGGEAQRLKLARALRGKVNGQLFVLDEPSAGLHAEDIQCLIAAMHDLV
                     ERGASVLVVEHDLDVLRAADWIIDLGPGAGRHGGRVVDQGTPEHIAQGSQRTALALQQ
                     RHPTTEPSSHPQPLAKAHGNHAITIRNAREHNLRSLSCQIPHQRLVVVTGPSGSGKSS
                     LVFDVMFAQGQRRFLETLTPYARQFLPIMPRPDVDAVAGLPPAIALEQRRSRTGGQST
                     VATVTEVAHYLRLLFAKVGVAHCPDCDVAISALTMDSLLASLREQKGKGMLLAPVVQA
                     RKGVYLDLLTSAHRAGIAWARVDGEKVSTDNPPKLRKTKEHDIDLLLWEGNLRDTPRE
                     AVEKALSWAKGVVRITTLDGKEKLLSTRRACPSCGKGIPELDPRWFSFNTQQGRCEAC
                     EGTGLQEMGSGEPCEVCAGARLAPLPRSVRLCGWRYSEVVALSVDSARKQIRRWSFSG
                     SDARLSEQALRELLRRIDFLSDVGLGYLSLDRDASTLSGGEMQRLRLSAQLGAGLTGA
                     LYVLDEPTIGLHPSDTQRLLDNLKALVATGSTVVVVEHDADTIRAADHVIDLGPGGGA
                     QGGRIMAEGKAEQVLGDKRSPTAVALGHRRLLEAPQRGVAKDYITVRGAEAHNLKRID
                     AAWPVGRMTVVAGVSGSGKSTLVQQILLPAVRAALGLTTPPPLAHDAVQGAAYVERAV
                     AVDQQPLGRTPRSIPATFLGVWDEVRRLFASLPESQVRGFGAARYSFNSHAGGRCSVC
                     GGMGVLSHEMSFLPDVITVCDACHGARFEPSTLEVRYRGLSVGDVLRMTANEAAELFE
                     AHPRIAAPLQMLEELGVGYVQLGQGSHTLSGGEAQRLKLALELTASVRHRPTLYVLDE
                     PTTGLHVSDVQKLIRVLHKLVDRGDTLVVIEHHPDVMVAADHILELGPRGGEQGGRVV
                     AAGPPREIANLQTPTAKVLKRIFNEKYEKYTPQISTLVNEYSRSTS"
     gene            complement(6161..7333)
                     /locus_tag="PK710_05675"
     CDS             complement(6161..7333)
                     /locus_tag="PK710_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09239.1"
                     /translation="MRHHLIVWAAGFMILYGCGDDDFAEDGREDSGSKSHQEGGAADV
                     VGSDGKAADSGIPSGEAGPQDSGPGPKDSGPGPSDGGSSPADSGPVGVCDDACTGAKM
                     CNDQGECVCAPGWTASASQCVATPLSHPATRTKADVCARFAEASQKPTALWKAGGGDE
                     CDPGTVPYEAQVAALNYLNFYRWMVGVGPVQVLPKIAKAEQECAKILGYSFGHSPPPT
                     TKCYTDEGAAACGASLIASGYDLMTQFDGYGMEIDQNLVHRRNILSVGRAGVWTGTSI
                     PGGSAMHYGGSYPALDSDPEYVAHPGSGPNVLSRVPKRWFVQKGTKSIPALDARVVEV
                     GSGTEKPMKRNHHYKDFSSFDLSGWSPVVDTAYRVELIDDDGVVFSTFETVFIQCP"
     gene            7651..8496
                     /gene="arsM"
                     /locus_tag="PK710_05680"
     CDS             7651..8496
                     /gene="arsM"
                     /locus_tag="PK710_05680"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOT09240.1"
                     /translation="MADNHDEIRQNVSEDYAQAVKKPTKSCCCSPTPKGVVAKLAGYS
                     HEQIAGLPTEAVVNSFGCGNPLAFSEIKEGQCVLDLGSGAGIDLLLAAKKVGPHGKVI
                     GVDMTDAMLEKARENIALAGLSNVELRKGIIEDLPVSSHSVDWVISNCVINLSPEKSK
                     VFSEIARVLKPGGKMIVSDIVAERLPPEISGHRQLTSCCLAGAISENAYLEGLRHAGL
                     TDVSVIDRLVYDRDQIEAFIGTELTIEESGCCGSTRISLAKTWAPRLEGSVASVKVSA
                     RKPCS"
     gene            complement(8516..10843)
                     /locus_tag="PK710_05685"
     CDS             complement(8516..10843)
                     /locus_tag="PK710_05685"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT09241.1"
                     /translation="MTVIFVNPPIRLPRAFAHYPMFSTLGMLTNAAWLRAQDISVQVV
                     DAFCLSAQLKLREDGGGFRHLGAEVDQVVAEVAQRAAMSDEPPIVIVALTMFSDMNRI
                     GENLVPQVVQGIRRVVPHASIGLADLYICGMNYFPFDVHAVMHAVAQADWVLVGEGEV
                     TLPELVRRLEARQSIEGLPRVAVRTQGDILYNPSAPQVFSDLDALPLPAFDLLNMEHY
                     FGVQAGALQADLVHEYHVVERQLPLMTSRSCPYRCHFCTNQVLGLPWRAHSVSYVRQA
                     VRTLRQQYGVQRFLWLDDNINVDAKRFRDLVLALEQEGVPWDAVNGYRADRLDREMVR
                     AIKAAGNTKITVSAESGDPTLLDKLIRKGLKLSSVVNLARVCEEERIPLQVHYIVGVP
                     GETKAQINQTLEFATVLFEKHGAWPLLQHAIPFPGTGLFRDCEEKGYFIAPPFEIAGS
                     VLEEESIIRTPEFEPAEVLRMKRNAQHLHAAMQKVAFASVTSRCDSDCLACHCHSSTV
                     AHASPSIEELCFDLERAQFLGAQEVILGGGEPTMRPDLPSIVTAIKNTGFHRVTLLTN
                     AHGLASAKRVQALSQAGVDAIAVDMLAHEATLHDDLTRMKGAWKLMVLGMNRARQAGW
                     SIDVHIPVLRANLSTLAETVQLAFRLGAKTVHFQIAPPPSPALKAGQIPRWNEAVGPM
                     LRAIGGSRRGSTWIQGAPLCLLPSRKGLLRPLPPWALQASRAHKMKHAVCRQCVAYLL
                     CGGFFRPEYEPAYGMMAALGLEASTQSDEDSTEPT"
     gene            complement(10840..12675)
                     /locus_tag="PK710_05690"
     CDS             complement(10840..12675)
                     /locus_tag="PK710_05690"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOT09242.1"
                     /translation="MAWMIQPAIVALGGPPNTRFVGISHPIRLDPRSLEAIETDLRTA
                     HRQGARAVWLAGGEPLMRQDLHKILRSCLDRRLVPAILTNGRMLVYAKIRQLIDRAKV
                     GALRIELHGPRDVHDRLVGVAGAFDQTVEGLRALLIDASRSLHIEVACTVLAENLPSL
                     TQWVDEIATLPRRIPLTFRFVAPLILEDSAQWPRSEQVIEHLGDALRNAQRSGADLAT
                     AWEGFAPCLLSDHASSRDDSLRQHALVLGPREATAPFPLEDADCRTFPLPCQDCIHQP
                     TCPGAPHNLLVFEGESGLRPTRSLRANSFNFELVQSIHPFHLQPGACTAQTIKARTTP
                     ARTVFLVDDSNASLYESPTSDFTDSEIAYVKDEVEQVYIDIEPGASLTDFIHGVKRTR
                     FDPVCASCPDRPRCGGAMRIDPSPPFEREERWLSKEVSRLRGRVLDVGCGDSLYRDQL
                     RTLIESGAIEYHALDPDDTALERLKAEVPGIFHRSEIENFHFEPGYFDYVLVLRSLNH
                     FKDIAKSFEVISTVLRVQGQMVLCDSPPFAMLRTPRQVSYADEHAPVGHEHYRNWTSH
                     QVVEFLKRYPFRVDVHRPVSVHTSNEWILKVMRMTNTGDCGPERS"
     gene            complement(12686..13597)
                     /locus_tag="PK710_05695"
     CDS             complement(12686..13597)
                     /locus_tag="PK710_05695"
                     /inference="COORDINATES: protein motif:HMM:NF014312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HOT09243.1"
                     /translation="MPPAYPTGAALPPRAYVVGGGSDNVTVIDTSSHQVIGQVVVGDE
                     PMLPALSPDGRLLLVTNRDSMNATVIDTASLTVVHTVDVQDGASGCAFSTDGTLAYVA
                     NEYSHTVSFIDTATWQPLRHVPVGHGPCDLRVTPNGKHVIVTNEESHNIAVLEATTGQ
                     VQHIIPTPDGPLGLTIDPTGSFFLAACFIANHLVAVETRSWQVAWKVPVGSCHVGLCL
                     AHQGRVALITNADQDHLSVFDLKRRKEVSRIRVRANPAMVQVIPGSDLAYVTDRGADS
                     VSVVDVRACKTIHTIPVGSSPLGVCIG"
     gene            complement(13657..>14301)
                     /locus_tag="PK710_05700"
     CDS             complement(13657..>14301)
                     /locus_tag="PK710_05700"
                     /inference="COORDINATES: protein motif:HMM:NF019610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09244.1"
                     /translation="PRYCNTLYFNNLQDKRPASSDQLPPVHLPVHLIPFGPVPQETLE
                     HAAQGLEQHAPVHVTLHEERPFPEYAKSSRKGAYQASRLLDALASLPHDGKLMGITEA
                     DIVTRKGSVPVWGILGMGAIDGRVCVISTYRMRRRWEGRGASEALVRERLWKTAIHEL
                     GHTLGLEHCPKVGCLMEDGHGTVKTIDRDTALCDECARKFEASLLRNAQHKSDD"
BASE COUNT         3019 a         3952 c         4358 g         2972 t
ORIGIN      
        1 gcgttcgggt ccgcatgcta gagcaagacg ttggtccacg acgatatcgg atgacgtatc
       61 acctggtcga cggcgcagcg attccggggg gggccgtcga tcggctcgct cggctggtgg
      121 aagccatggt gcgaaacgga gaacaccggg cagcagctta tttggcggat cgaaggggtg
      181 gcaggaagaa atgaggcttt cgtggagcag cggtcagtct acgttccaaa ccatcgggtt
      241 gaggtgatcc gtgcgcaggc tggcaacggt tttcaaagcg ctttctgatg aaacacgcct
      301 tgccatgctc gggcttctgc ttcgggaagg cgaattatgc gtatgtgatt tcgtagaagt
      361 cctcggaatc acacaatcca aagcctcgcg gcatttgcgc tacctggtcc acgcgggtct
      421 gcttcaagat cgtcgggaat tgacctgggt ctattaccgc atggcggacc aactcggcgc
      481 ggagcaaagg ttggtgttat cgggggtgca ggaattggta aagcaacaac tgcctgatga
      541 gttgcttgaa tctctaaaaa catggaaaca gcagaaacag tgcgatggtg gatgtggtcc
      601 atcgccgaaa aagcgactct cgagctgtcg cggaaggtgc gaggggacga tcgttaggcc
      661 cctggctctt tttgaaaacg gcattatgaa gcgatcccct cgctcatgaa tcgcaccgtt
      721 cttcgcaacg cccgaacgca taacctacag tccgtctgta tcgagctgga gcccggccaa
      781 ctcatcgccg tgacgggagt atccggttct ggcaaatcgt cgttatgcct cgatacgctt
      841 tatgcggagg gtcagcgtcg tttcgtggag agtttcagtc cgtacgcgag gcaattttta
      901 gagcgccatg agcgtcctcc gatcgacagt ctggaccctg ttcccgcggc gatcgctgtg
      961 gatcgtcatg ctcctgtgaa aagttcacgg tcgacggtag cgaccatgtc cgacgtggaa
     1021 ccctatcttt ccgcgctatt tttgcgggaa gggcggccga tctgtccgaa ctgtgcggtg
     1081 ggcgcgacgt ggatggatgc gggggtggcg gcgtcttcgt tggtgtggga gtgggcagac
     1141 gagcgggtca tcgtgttggc gccgggccgg gccgagggaa cggcggcgta tctcgatgcg
     1201 cgcgacaggc ttgtggcggc ggggcatcgt cgtttgtggc tggccgattc gattcgcgac
     1261 atcgatacgg ttcggccgag tgagattttg tcggaaggct cgcgcttcga agtcgtcatc
     1321 gaccgaatca agctgggaga accatcgtta cgaagactgc aagaagcgct ggaacaagct
     1381 tggaccatga cccaaggtca agcggtgatc gtgggggaaa gcaaagggcg tcggaccgtg
     1441 caacgggggc tggtatgtcc ctcatgtgcc aagtcctttg ctccaccacg cccggggctt
     1501 ttttcctatc aatctccggt gggggcttgc gaagaatgtc acggcttcgg acgtgtcata
     1561 ggtattgatt ttcaaaaggt ttttccggat gccgccaagt cgcttgccca aggggccatt
     1621 cgtccctggt ctgggaaaac cagcgcttgg gagcgtcgaa cgctgaagaa attctgctta
     1681 cgtcatgata tcgacatgga tcggccttgg ggacaactga cgaaaaagca gcaacaattg
     1741 gtgattacgg gggatggtac gtggagcggt ggaaaatacc cgggaattat cgcgtggttt
     1801 cggtacttgg agtccaaagc ctataaaatg catgttaggg tgatgctttc gcggtaccgc
     1861 gcctacgatc cttgcccggt ttgccgtgga aaacgcctca atgccgccgc gttatcctat
     1921 ggtgtcgacg gtctcgacct agctgggtgg cacggtttgg agcttgggga agctcgcgcg
     1981 cgattgatgg gatggaaagc cacccatccg cagggggaac tcattcgcaa ggagcttctg
     2041 tcgcggctcg gttttctttg cgatgtgggg cttggatacc tgactctcga ccgacaagcc
     2101 cggaccttgt cgggagggga agcccaacgc gtatcgctga ccgccgccct tggtgcctcg
     2161 ctttctggtg cgctcatcgt attggatgag cccaccgtgg ggctgcatcc gagcgatata
     2221 tcgccgctcg tagactgcat gcgctcgctc gccgaccatg gcaatacggt ggttgtggtg
     2281 gagcacgatc cttcggtggt tagggcggtg gatcgggtgg tagaattggg gcccggggca
     2341 ggggcgaagg ggggaagaat ccttttcgaa gggtctccag agcagctcac tgcaaggacc
     2401 gatttgccca cggggcgcgc gctggcggcc tggcaggaaa accagccgtc gaatcgtcgg
     2461 aagtggcaca aaacgttgcg tgttcataat gctcgagcca acaatttggc cactatcgat
     2521 gtggatattc ctctgggggt gatatgcgct ttgagcggcc cgagtgggtc gggaaaaagc
     2581 accttggcgg aagagatcgt gtaccgggcc gtggctcgcg cccatggcat gacggatgtg
     2641 gatccgcctg cggagcacga tcgcctggag ggggtcgagc atctttgcgg ggttacgttg
     2701 gtggatcaga acccgctggg aagaacgtcg cggggcaatc ccgcaaccta taccaaggcc
     2761 tgggatcggg tgcgtgcgct atttgcttcc gaacccgcgg ctcagggcct ggggttcacc
     2821 ccttctcact tttcattcaa tgttgccgct gggcgctgtg atgcttgcgc gggggagggc
     2881 ttcgagacgg tagagatgca gtttctcgcg gatatctcgc tgttatgccc cgtatgtcgt
     2941 ggaaaacgct tcaaatcgga agtgctggag gttgtgcatc atggcaaaac ggtggcggat
     3001 ttgcttgcca tgacggtcga tgaggtagtg caatgggcca accatgatcc ggccctggtt
     3061 cgcgcccttc gccctttgca atccctgggg cttggctacc tgcctctcgg acaacccctg
     3121 tctactttat cgggagggga ggcgcaacgt ttgaagttgg cgcgagcctt gcgcggaaaa
     3181 gtcaacggac aactttttgt gctcgatgag ccgagcgccg gccttcatgc cgaggatatc
     3241 cagtgtctga tcgccgccat gcatgatctg gtggaaaggg gcgcaagcgt gctcgtggtc
     3301 gagcacgacc tggatgtgct gcgggccgcc gattggatca tcgacctggg ccccggcgcc
     3361 ggacgtcatg gcggaagggt tgtcgaccaa ggaaccccag agcatattgc ccaaggttcc
     3421 caacgcacgg ctctcgccct gcaacaacgg catccgacga cggagccgtc gagccacccc
     3481 cagccgctcg ccaaagccca tggcaaccat gccatcacca ttcggaacgc tcgagagcat
     3541 aatttgcgca gcctatcgtg ccagataccg catcaacgcc tcgtggtggt caccgggcca
     3601 agcggatcgg gcaaaagctc gctcgttttc gatgtgatgt ttgcgcaagg gcaacgccgc
     3661 tttctggaaa cccttacccc ttatgcgcgg caatttctgc ccatcatgcc ccgtcccgat
     3721 gtcgatgcgg tggcgggtct gcctcccgcg attgccctgg agcagcgacg ctcgaggacc
     3781 gggggccagt cgaccgtggc taccgtcacc gaggtcgccc attaccttcg tttgctcttc
     3841 gccaaagtgg gtgtggctca ttgcccggat tgtgatgtcg cgatatcggc acttacgatg
     3901 gactcgctgc tggcttccct tcgagaacaa aagggcaaag gaatgctgct ggctcccgtc
     3961 gtccaagcgc gaaaaggagt ctacctggac cttttgacct ccgcccatcg cgcgggaatc
     4021 gcttgggctc gggtggatgg cgaaaaagtc agtaccgaca atcctccgaa actgcgaaaa
     4081 accaaggagc atgatatcga tctcctcctg tgggaaggta acctgcgaga tacgcctcgg
     4141 gaggcggtgg aaaaagccct ttcctgggcc aaaggggtcg tacgaatcac gacgctagat
     4201 ggtaaagaaa aattactatc gacccgtcgt gcatgcccca gttgcggaaa aggaataccc
     4261 gagctggacc cccggtggtt ttccttcaac acgcaacaag ggcgctgcga ggcttgtgaa
     4321 gggacagggt tgcaggaaat gggatcgggc gagccttgcg aggtttgcgc aggggcgcga
     4381 ttggctcctt tgccacggtc ggtgcgcctt tgcggatggc gatattcgga agtggtggca
     4441 ctatcggtgg acagcgctcg aaaacaaatc cgacgctggt cattttcggg aagcgatgcc
     4501 cgtctgtcgg agcaggctct tcgcgaattg ttgcgtcgta tcgacttttt atccgatgta
     4561 ggccttggct acttgagcct cgaccgcgat gcttcaaccc tgtcgggcgg tgaaatgcag
     4621 cgtttgcgtc tgtccgcgca gcttggggcg gggttgaccg gtgcgcttta cgtgctcgat
     4681 gagcccacca ttggtttgca cccctccgat acgcaacgtt tgctggacaa tctgaaagcg
     4741 ctcgtggcta ccggctcgac ggtcgtggtg gtggaacacg atgccgatac gattcgagcc
     4801 gcggatcacg tcatcgacct gggaccagga ggcggagccc aggggggacg tatcatggcg
     4861 gaaggaaagg ctgagcaggt gctgggcgac aagcgttcgc cgacggctgt ggcgcttgga
     4921 catcgcaggt tgctcgaagc gccccaaaga ggcgttgcga aagactatat caccgtgcgg
     4981 ggggccgagg ctcacaatct caagcggatc gatgccgcct ggcccgtggg acgaatgacc
     5041 gtggtggcgg gggtatccgg ctcgggaaag agcacgctcg ttcaacagat tctccttcct
     5101 gcggtgcgag cggcattggg gcttacgacg ccacccccgc ttgctcacga tgcggtgcag
     5161 ggagccgctt atgtcgagcg tgcggtggcg gtggatcagc agcccctcgg ccgcacccca
     5221 cgatcgattc ccgccacgtt tttaggggtt tgggacgaag taagacgcct tttcgcgtcg
     5281 ctgccggagt cgcaggttcg tgggtttggc gcggcgagat attcgttcaa tagccacgcg
     5341 gggggacgct gctcggtgtg tgggggcatg ggcgtgcttt cccacgaaat gtcgtttttg
     5401 cccgatgtca tcacggtctg tgatgcttgc catggggcgc gcttcgaacc ttcgacgctg
     5461 gaagtgcgtt atcgggggct gtccgtgggc gatgtgcttc ggatgacagc caacgaagcc
     5521 gcggagctat tcgaagcgca tcctcggatc gcggcgccac ttcagatgct ggaggaattg
     5581 ggggtaggct atgtgcaact gggacaaggt tcccacaccc tttcgggagg agaggcgcaa
     5641 cgtttgaaat tggctctgga attgacggcg agcgtacgtc atcgcccaac gttatatgtt
     5701 ctcgacgaac ccaccaccgg gctgcatgta tcggatgtgc aaaaactcat tcgagtgttg
     5761 cataaactcg tggaccgggg cgatacgctg gtggtgatcg agcatcaccc ggatgtgatg
     5821 gtcgcggcgg accatattct ggagttgggc ccacggggag gtgagcaggg agggcgtgtg
     5881 gtggccgcag gccccccccg cgagatcgcg aacctgcaaa cgcccacggc aaaggtgcta
     5941 aaacgcatat ttaacgaaaa atatgaaaaa tatacgcccc agatatcaac ccttgtgaac
     6001 gagtattctc gctcgacttc gtaggcccgg gatccccaat cccaaaagag attcccccta
     6061 ttgaagaggc cgccagcgcc tctcctgtgc gcaggggtgc gacgtcccca aagacctgta
     6121 ggtaagggct attcttttcg gtgttctggg gcgggccatc tcaagggcat tggatgaata
     6181 ccgtttcaaa ggtggaaaac acgacaccgt catcgtcgat gagttcgact cggtaggcag
     6241 tatcgacgac cggactccag cccgaaaggt cgaaagagct gaagtctttg tagtgatgat
     6301 tgcgtttcat cggtttttcc gttccggacc caacctcgac gacgcgggca tcgagcgcgg
     6361 gaatgctttt cgtgcctttt tggacgaacc agcgttttgg gacgcgcgaa aggacgttgg
     6421 ggccggagcc ggggtgcgcc acatactccg gatcgctgtc gagggccggg tagctgcccc
     6481 cataatgcat cgcggaaccg ccgggaatgc tcgtacccgt ccagacaccc gcgcgcccca
     6541 ccgacaagat attgcgtcga tgcacgaggt tctggtcgat ttccatccca taaccatcga
     6601 attgcgtcat gagatcatag ccactggcaa tcaacgaggc tccacaagcc gccgcgcctt
     6661 catcggtgta gcacttggtg gtgggcggcg gggaatggcc gaaagaatat cccaagatct
     6721 tggcgcattc ttgttcagcc ttggcaatct tgggaagcac ctggacgggc ccgacaccga
     6781 ccatccatcg gtaaaagttg agataattga gcgcggccac ctgtgcttca taaggtacgg
     6841 tgcccggatc acattcatcc ccgcctcccg ccttccatag tgccgtaggt ttttgggagg
     6901 cttccgcgaa gcgagcgcaa acgtcggctt tcgtgcgcgt ggcaggatgg gataggggcg
     6961 tggcaacgca ttgcgatgcc gatgccgtcc agcccggcgc acagacgcat tccccttgat
     7021 cgttgcacat tttcgctccg gtacacgcgt catcacagac acctaccggt cccgagtcgg
     7081 cgggggatga tccgccatcg ctcggacccg ggccggagtc tttggggcca gggccggaat
     7141 cctgcggacc tgcttccccg gaagggattc cggaatccgc tgctttgccg tctgaaccga
     7201 ccacatcggc ggcgcccccc tcctgatggc tcttcgaacc actatcctcg cggccatctt
     7261 ccgcgaaatc atcatccccg catccgtaca aaatcatgaa tccagccgcc caaacaatga
     7321 gatggtgacg catccctgaa ggctagcaca agcgctggcg tcgggaaccg ctcgaaattt
     7381 ttgttttcgc accctaatcc caggtggctc ttcacccacg gagctttcgg ttcaccggct
     7441 cgtcatgtct tgctacagcg acacaccctg cgcaacccct tcgctggggt tcgatattcg
     7501 gaagcaaaaa tattgaaaga gaggaatcaa gggaaaggtt ccgcgcgacc agggcatttc
     7561 gagcatgttg cgattggctc gtgctaaccc gcgagcacgg tagtatatat ggataaccgg
     7621 ttatacgata acaagagaag gattcccccc atggcggaca atcacgatga aatccggcaa
     7681 aacgtgtcgg aagactatgc gcaagccgtt aaaaaaccga cgaaatcttg ttgctgctcg
     7741 ccaaccccca aaggtgtcgt cgccaaactc gccggctatt cccacgagca aatcgcgggc
     7801 cttcccactg aggccgtcgt caattccttc ggctgcggta accccctggc tttttccgaa
     7861 atcaaagaag gacaatgcgt gctcgacctc ggctccggcg cgggcatcga cttgctgctc
     7921 gccgccaaaa aagtcggccc gcatggcaag gtcatcggtg tcgatatgac cgacgccatg
     7981 ctcgaaaaag cacgcgaaaa tatagccctc gccggtctgt ccaacgtcga actccgaaaa
     8041 ggcatcatcg aagaccttcc cgtgtcctcc catagcgtcg attgggtgat ctccaattgc
     8101 gtcatcaatc tatccccaga aaaatcgaag gtattttccg aaatcgccag agtcctcaaa
     8161 cccggaggga aaatgatcgt ttcggatatc gtcgccgaac gattgcctcc cgaaatctcc
     8221 ggccatcgac agctaacgtc gtgttgcctg gccggggcta tttctgaaaa tgcatatttg
     8281 gaaggccttc gccatgctgg gcttaccgac gtttccgtga tcgatcggct cgtttacgat
     8341 cgcgatcaaa tagaggcgtt cattgggacg gaactaacca tcgaggaatc ggggtgctgt
     8401 gggtcgactc gcatcagcct cgccaaaacc tgggccccgc ggttggaagg cagcgtggcg
     8461 agcgtcaaag tcagcgcccg caaaccttgc tcgtgaatca ccggtggatc gatactcacg
     8521 tcggttcggt cgaatcttca tcgctttgtg tggacgcctc caaccccagc gctgccatca
     8581 tgccgtaggc gggctcgtat tctggcctga aaaaaccgcc acaaagaaga taagccacgc
     8641 attgcctgca cacggcatgc ttcatcttat gcgcgcgaga agcctgcaag gcccaagggg
     8701 gcaaagggcg aagcaagcct ttgcgcgagg ggagcaaaca caaaggcgcg ccttgaatcc
     8761 acgtcgagcc gcgacgcgac ccgcctatcg ctcgcagcat cgggcctacc gcctcgttcc
     8821 aacgaggtat ttgcccggct ttcagcgccg gactgggggg cggggcgatc tgaaaatgga
     8881 ctgttttggc gccaagacga aacgcaagct ggaccgtttc ggcgagggta gatagattgg
     8941 cacgaagcac gggaatgtgg acgtcgatcg accagcccgc ctgtctggct cgattcatcc
     9001 caaggaccat gagcttccac gcgcctttca tgcgcgtgag atcgtcgtgc aacgttgcct
     9061 catgggccag catgtcgacc gcgatggcat cgactcctgc ttgggagagc gcctgaacgc
     9121 gtttggccga agcgagacca tgcgcattgg tcaagagcgt cacgcgatga aagcccgtgt
     9181 ttttaatcgc ggtgacgata gaaggaaggt ccgggcgcat ggtgggctcc ccaccaccca
     9241 ggatgacttc ctgggcaccc aaaaattgag cccgctcgag gtcgaaacac agctcttcga
     9301 tgctgggtga agcgtgggca acggtcgagc tgtggcaatg gcaagcgaga caatcgctgt
     9361 cgcagcgtga ggttacgctc gcgaaagcaa ccttctgcat ggccgcgtgc agatgctgtg
     9421 cattgcgctt catccgcaac acctccgccg gttcgaactc cggtgttcgg atgatgcttt
     9481 cttcctccag cacgcttccc gcgatttcga aaggaggtgc gataaaatat cctttctcct
     9541 cacaatcgcg gaaaagccct gtgcccggga agggaatcgc atgctggagc aaaggccagg
     9601 caccgtgttt ttcgaataat acggtcgcaa actcgagcgt ctggttgatc tgggccttcg
     9661 tttcgcccgg cacgccaacg atataatgca cctgcaacgg gattcgttcc tcttcgcaga
     9721 cgcgcgcaag attcaccacc gacgataatt tgaggccttt tcggatcaat ttgtcgagca
     9781 gagtgggatc gcctgactcc gcggaaaccg tgatcttggt gtttcccgcg gctttgatgg
     9841 ctcgcaccat ctcacgatcc aatcgatcgg cgcgatatcc gttgaccgca tcccatggca
     9901 cgccttcttg ttcaagggcc agaaccagat cgcgaaatcg tttggcgtcg acgttgatat
     9961 tgtcatcgag ccatagaaat cgctgtaccc catactgttg tcgcaaggtt cgaacggcct
    10021 gtcgaacgta ggatacggaa tgggcgcgcc agggcagtcc aagcacctgg ttggtgcaaa
    10081 agtggcagcg atacggacag gatcggctcg tcatcagggg aagttgccgt tccaccacgt
    10141 gatattcatg caccagatcc gcttgcaacg cccctgcttg cacaccgaaa taatgctcca
    10201 tgttcaataa gtcgaaggcc ggaagaggca gcgcgtccag atcggaaaaa acttgcggcg
    10261 cggagggatt gtaaaggatg tctccttggg ttcgcaccgc gactctgggc aatccctcga
    10321 tggattgtcg ggcctcgagc ctgcgtacca actcgggtag cgtgacctcc ccttcaccca
    10381 ccaacaccca atccgcttgt gcaaccgcat gcatcacggc atgcacatcg aacggaaagt
    10441 aattcattcc gcagatatac aagtccgcca ggccaatcga tgcatggggc accacgcgac
    10501 gaattccctg cacgacctga ggtacgagat tctctccgat tcgattcata tcggaaaaca
    10561 ttgtcaacgc gacgatcacg atcggaggct cgtccgacat cgccgcgcgc tgcgccactt
    10621 cggcaacgac ctgatcgacc tctgcgccaa gatgccgaaa cccaccccca tcttcgcgaa
    10681 gcttcaattg cgcggacagg caaaacgcgt ctaccacctg caccgaaatg tcctgcgctc
    10741 gcagccatgc ggcgttcgtc agcattccca aggtgctgaa catcggataa tgcgcaaaag
    10801 cgcgcggtaa acgaatggga ggattgacga aaatgacagt cacgagcgct ccggtccgca
    10861 atctccggtg ttcgtcatgc gcatgacttt gagtatccat tcattgctgg tgtggacact
    10921 cacgggacga tgcacatcca cccggaacgg gtaacgtttg agaaattcga cgacttggtg
    10981 gctggtccaa ttacggtaat gctcgtgacc cacgggagca tgctcgtcgg cgtaggatac
    11041 ttgccgcggc gtgcgaagca tcgcgaacgg tggagaatcg cataaaacca tctgcccctg
    11101 cacgcgaagc acggtcgaaa tcacctcgaa ggatttggct atatccttga aatgattgag
    11161 agatctcaaa accaacacgt agtcgaagta gcccggttca aagtggaagt tttcgatttc
    11221 cgaacggtga aagatcccag gaacctcggc tttcagtcgc tccaacgctg tgtcgtcggg
    11281 atcgagagca tggtattcga tcgcaccgct ttcgatgagg gtccgtaatt ggtcgcggta
    11341 caacgaatcg ccacagccga catcgaggac ccgtccccgc agacgggata cctccttgct
    11401 gagccaacgc tcttctcttt cgaaaggggg ggatggatcg atgcgcatgg cgccgccgca
    11461 tcttggccga tcggggcagg aagcgcagac gggatcgaag cgtgttcgct tcacgccgtg
    11521 gatgaaatcc gtcaaggaag cgccgggttc gatgtcgata tacacctgct ccacctcatc
    11581 cttgacgtaa gcaatctcgg aatccgtaaa gtccgaagtc ggggattcat aaagggaagc
    11641 gtttgaatca tcgacgagaa agaccgttcg tgcgggggtc gttcgcgcct tgatggtttg
    11701 ggcagtacat gctcccggct gaagatgaaa cggatggatg gattgaacca actcgaagtt
    11761 gaacgaattg gctcgaaggc ttcgggtggg tcgaagcccg ctttccccct cgaatacgag
    11821 aagattatgg ggcgcgcccg ggcaggttgg ttggtgaatg caatcctgac agggcagagg
    11881 aaaggtgcga caatccgcat cttcgagggg aaacggagcg gttgcttcgc gcgggcccaa
    11941 cacaagggca tgctggcgaa gtgaatcgtc tcgcgaagac gcgtgatcgg aaaggagaca
    12001 tggcgcaaat ccttcccacg cggtcgcaag atccgctccc gagcgctgtg cgttcctcaa
    12061 agcgtcgccc agatgctcga tgacctgttc cgaacggggc cattgtgcgg aatcctcgag
    12121 tatcaagggt gcgacgaagc gaaaggtcag agggatgcgg cgaggcaacg ttgcgatttc
    12181 atccacccac tgggtcaacg aagggagatt ttctgcgagt actgtacagg caacttcgat
    12241 gtgcagcgag cgggaggcat cgatcagcag cgcgcgcaat ccttccacgg tctggtcaaa
    12301 tgctcctgcc acgccgacga gccgatcgtg aacatctcgt ggtccatgca attcgatgcg
    12361 aagggcaccg actttggcgc gatcgatgag ttgccggatt tttgcataca cgagcatgcg
    12421 accgttggtg aggatggcgg gcaccagtcg tcgatcgaga caagagcgta gaattttgtg
    12481 cagatcttga cgcatcaaag gttctccccc cgcgagccag acggcgcgcg caccttgtcg
    12541 atgcgcggtg cgcaagtctg tttcgatcgc ttcgaggctt cgggggtcga gacgaatcgg
    12601 atgcgatatc cctacgaatc gcgtgtttgg cggtccacca agggcaacga tggcaggctg
    12661 aatcatccag gccatgactt ccttattacc cgatgcatac accgagggga gaacttccta
    12721 cgggaatggt atggatggtt ttgcaggctc gaacatccac gacggagaca gagtcggctc
    12781 ctcggtcggt tacgtaggcc aaatcgcttc cgggaatgac ttgcaccatg gctggattgg
    12841 cccggacacg aatacgagac acttccttgc gtcgcttcag gtcgaaaacc gaaagatgat
    12901 cctgatccgc attggtgatc agcgcaacac gtccttgatg ggcaaggcaa agacccacgt
    12961 ggcacgagcc gaccggtact ttccaagcaa cttgccaaga acgggtttcc acggccacca
    13021 gatggttggc gatgaaacaa gccgcgagaa aaaacgagcc cgttggatcg atggtaagcc
    13081 ccaaaggccc atcgggtgtg gggatgatat gttgcacttg ccccgtggtt gcttcgagca
    13141 ctgcaatgtt atgagattcc tcgttggtga cgatcacatg cttgccattg ggagtcaccc
    13201 gcaaatcgca gggaccatgg ccgacaggaa cgtgtcggag cggctgccag gtagcagtat
    13261 cgatgaacga aacggtatgg gagtattcgt tggccacata agcgagcgtt ccatcggtgg
    13321 aaaaagcgca accggaagcc ccgtcttgga catccacggt gtgaacgacc gtcagcgaag
    13381 cggtgtcgat cacggtagcg ttcatgctat cgcgattggt gacgagaagc agacgaccgt
    13441 cgggcgaaag ggcagggagc atgggttcat cccccacgac tacctgtcca atgacttggt
    13501 gtgacgaggt gtcgatgaca gtcacgttat cagagccgcc gccgaccacg taagctctgg
    13561 ggggtagcgc tgcaccggtt ggatacgcag gaggcatcgg accgagtatg gtcgtgaatg
    13621 acaaatcagc aaagcattgg cgcgtcgaag ggccgctcaa tcatccgatt tgtgctgcgc
    13681 gtttcgaagt aacgacgctt cgaatttccg cgcacattcg tcgcatagcg ctgtgtctcg
    13741 atcgatggtt ttcaccgtgc catgcccgtc ttccatgagg caaccgacct tcgggcagtg
    13801 ctctaggccc aaggtatgac ccaactcgtg aatggcggtt ttccacagtc tttcgcgaac
    13861 caacgcctcg gaagcgcccc gcccctccca ccggcgtcgc attcgatagg tagaaatcac
    13921 gcaaacccgt ccatcgatgg cgcccatgcc aagaattccc cacaccggca cactcccttt
    13981 gcgggtgacg atatccgctt cggtaatgcc catgagtttg ccgtcgtggg gtaaggaagc
    14041 cagggcatcg agaaggcggc tggcctggta cgcacctttt cgggaggatt tcgcgtattc
    14101 agggaaaggt cgttcttcgt gaagcgtcac gtgaaccggc gcgtgctgtt cgaggccttg
    14161 ggccgcatgt tccaaggttt cttggggaac gggtccgaag ggaatgaggt gaacgggcag
    14221 gtggaccggc gggagctggt cggatgaggc tggtcgttta tcttgtaagt tgttgaaata
    14281 aagggtatta caatatctgg g
//
LOCUS       DAOSOR010000070        14226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4305_length_14226_cov_4.870016, whole genome shotgun sequence.
ACCESSION   DAOSOR010000070 DAOSOR010000000
VERSION     DAOSOR010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 14226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14226
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4305_length_14226_cov_4.870016"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="PK710_05705"
     CDS             complement(<1..911)
                     /locus_tag="PK710_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09245.1"
                     /translation="MKEHARSLIKRVRDVVSDHRQRTVASDVLQMCIMAMEQLAELDQ
                     TLYDYFANAKLSLTDHDELQAGITEATFVEVHAKTFRAIRKLCSYLHASGMLRHSERP
                     STLDNQGFDFGDDFDLCFEEASPAGAPTLEDLEIDEIDKAFEAIADEIPDSSTMLGLF
                     REQLGTLGGVLTQQAETFDLRIAIAIERRNFELALRELDASRQALGDGIFAMASTVFE
                     IFGVNVERSDLVPSYKNTLEQSLELRKALSELSQLVTSENDWVIKDASMSAADVQEAI
                     FRVADVLEQYVDGNVCKGMRAPERLEL"
     gene            complement(1015..1479)
                     /locus_tag="PK710_05710"
     CDS             complement(1015..1479)
                     /locus_tag="PK710_05710"
                     /inference="COORDINATES: protein motif:HMM:NF019247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HOT09246.1"
                     /translation="MNWRPQPGKQHEKLHTLVGNWEGIVRLAPSAWGSAGMAVGRFSN
                     TVVADGFFLVSDYVEQRDGGETFVGHGVYGWDDIEKEYVLYWFDSMGLAPRQPARGTW
                     KENELVFVLEQPGGKVRYSHTFLEPGAYVFRLQSQRDDADWDTIMEAEYRRR"
     gene            1552..3384
                     /locus_tag="PK710_05715"
     CDS             1552..3384
                     /locus_tag="PK710_05715"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOT09247.1"
                     /translation="MAVDDPSNCRPNRCRIATATRFGSWFSCFIALGCSAAPVTSPQN
                     PQATLALPEAAKTLSLGRRQVAVAASPTPLLERLASEVQQFGTVFSSREDTPVYHLAY
                     EVNDTSKVDILASYGAILSHTHTRRRLLDVDLRVGSYELDNTHPLIDSAYYQGNAAIA
                     WDDDAHESLHNTLWWETNQAYKKACKELIEVRAQRQIDVASDDQSSDYSEEAPLVHLE
                     APVALTVDRADWEQRVRSLSALFRGHEHIHQGFVRFELTTTNRSYVSMGGHRVQTGTT
                     HARVSFGASGISDDGDELTRSDEIDAHDVSGLPNDEHLRFRVQHIIEDMEGLAKAPKA
                     EPYVGPALVDGKAAAVLFHEVFGHRVEGHRQKGQIEGQTFIDQVGQPIMNSVFDVYDD
                     PTVSSVNGIDLNGFYRVDSEGVRARRAQLIEKGVFVGFLMSRMPIRGMAHSNGHGRRE
                     PGYRAIARQANLIVDPSRVTSREALERALIDEVKRQRKPFGLRIGEVTGGYTTTQRGD
                     PQAFVVEPVMVYRVYPDGRQELVRGITLEGTPLSLLSHVLAAGNDFAVFNGFCGAESG
                     EVPASAISPSLLLRQIEVSRQPVSGQRPPLLPAPQIARSSGRKP"
     gene            3381..5117
                     /locus_tag="PK710_05720"
     CDS             3381..5117
                     /locus_tag="PK710_05720"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOT09248.1"
                     /translation="MKQRFIKALRIAAAGIGTVAIPIISHAQIPSHPISVSPWVSPMQ
                     DEIERAMSLELPGMPRPHYIIATLIEQQEALVEASLGAVLQQHQDRSRVLKVEVRVGD
                     RSFDNSNFLGGSPQAIVVWPAPLDDDPLCLRRLLWLAADRSYIGATESYEAKRAHREA
                     MTIEQDRIDDFSASAAVRFRGSSAPVVPPLDRLSALATAASSVFSNHPKVYQSRVRMA
                     VRSQARTLLDSENRVIEDSASLVEIEIVAQTQARDGMELSDRHAFASIHFDEIPTEET
                     MVRWARELMVRLETMREAAIVQPYSGPVLFEGRASSRLFRTLLAEELSATPSPEPTEG
                     QDTLGALATRKGFRILPVTFDVVDDPTVDRIAGLPAIGHYLFDDQGVRAQPVHVVKEG
                     ILDMLLSSRTPSKTVAISNGHGRAGWLGDARGRAANVWIRARDGLSRASLRNALLEAA
                     RKEGLDYGIIVAGFEESSGIEGTSFSLGRRDSQWPPSAEAYRLYRGGKLEPIRGLTIQ
                     GLRPRDLRRIVAAGADANVYSYRASANAVRAFNDSVGEIPTTIIAPSVLFPDVDVVGM
                     APPYPLVPAAAP"
     gene            5199..5735
                     /locus_tag="PK710_05725"
     CDS             5199..5735
                     /locus_tag="PK710_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09249.1"
                     /translation="MRAWVVSAVMLMGAYGLWMSSGIHREAPLVEHRGMTAGVVAMEN
                     EVALAPDDAQKLSSLCQAYLQRNAPGLALAAIHRAPSMVQQQPEIQHLWAKALLYEGQ
                     ASEALDKQRFVLAACEKQECSAWLVASAARQEAFLSALVDGGVEDVFRNPGQAFEAYR
                     LISGPMVTVMDSERQTVQ"
     gene            complement(5793..6362)
                     /locus_tag="PK710_05730"
     CDS             complement(5793..6362)
                     /locus_tag="PK710_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09250.1"
                     /translation="MGNTSSWDRSSTNTPVPIEVVDGAQTCVQYVQKNLGIALDYQTE
                     TLPVLDHYLREARAQGSPHTHALIASVAGCYLGELLRARHPLCWEPLAQDQLLWSLSA
                     PSITIFPVAMAHVALVGPKAEKQWEVFRFAPPLRQALTRRLQALPVVSDDEYVAPSTR
                     VEVVDMAFEWLEATNNKHAHDHEPAEPEE"
     gene            6501..7238
                     /locus_tag="PK710_05735"
     CDS             6501..7238
                     /locus_tag="PK710_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT09251.1"
                     /translation="MRIAIFSDVHANYEALSAVIEAYKREEIDVYYCLGDTVGYGGSP
                     NHCAELVRKLASKTILGNHDAAVAGRMDYSYYYEAARNALDTHAAMLSGESMSWLKNL
                     PYQERLIDQDLQLCHGSPIRLEEFEYIFAPEQARECLSIWDELQHLTVIGHSHLCKVF
                     ALDQDSVEELPATDLVLDPHRKYIISVGSVGQPRDYDNRASYTVYDLATGRFEFKRIE
                     YDIETAADKVLKADLERNFAHRLYIGV"
     gene            7308..7397
                     /locus_tag="PK710_05740"
     tRNA            7308..7397
                     /locus_tag="PK710_05740"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7342..7344,aa:Ser,seq:cga)
     gene            7461..7545
                     /locus_tag="PK710_05745"
     tRNA            7461..7545
                     /locus_tag="PK710_05745"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7495..7497,aa:Ser,seq:gga)
     gene            7559..8524
                     /locus_tag="PK710_05750"
     CDS             7559..8524
                     /locus_tag="PK710_05750"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOT09252.1"
                     /translation="MTQTRRFSFHTLAFEARGSPRIDPTEAVGSTAMRVRWLIPWLAI
                     FACGGPPSATTPSSSTPATERPSDLTSDELRSYDAYLERFRSPCDNNQSLASCLSSAR
                     SCSSCRAAAQFVARSIRSGYTAKQVQARYQARFDPALVQIIDVRGAPSLGPDDAPVTI
                     VEFADFQCPFCAVSAPLLDALVELYTPHLRLVFKDFPIKYHPNALSTAQAGVAAHNQG
                     KFWQLHHLMFANREQLDPPDIERYARSLNLDMQRFVSDWESAATTQRVDADYQEGIRL
                     NVRGTPSFFINGRSFDIELFDFGGEDLLAWIELEIELTTGKPYQK"
     gene            8534..9277
                     /locus_tag="PK710_05755"
     CDS             8534..9277
                     /locus_tag="PK710_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOT09253.1"
                     /translation="MRGVSDVCGCFPTGLPYPGIAQLVRTTLLGVVQELDASLPWVAH
                     PIVMIDTETTGRKASEDRIIEIAIVKGRDGEILSRDSWLINPGCPIPEDSSAVHGILD
                     EHVESKPFFADVCDEIIEKLEGAIPAAYNASFDREFLLAEIQRLQRHDLRDVPAIRDR
                     VVWIDPLVWARHLFQEEKSRKLSAMAELLGVEMERAHRATDDASAALRLMYKMAEQNR
                     VPSAYGEFIQEQVRLSRIQGEARSGWRRS"
     gene            9321..11498
                     /locus_tag="PK710_05760"
     CDS             9321..11498
                     /locus_tag="PK710_05760"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOT09254.1"
                     /translation="MNQDPSATPTLTRYTLRVEGMTCAACSSRVQRKLDKLQGIESAS
                     VNLATHLATVMAKPELGEEQIRACVQAAGYGVEVVRHEDTAAALAQRPDDRPALRRLI
                     VAGALAAPLMVLGMAHVNAPWSFALQAILAFAVTYGAGWTIHSAAVRRALHLDTTMDT
                     LVSLGSTAAYVYSLAAWLSSDFHHVYFETAGGIVAFILVGRYLEARSKARATDSLRSL
                     FAMRPTRATLLRGDGEAVVPIEVVRVGDRVLVKPGERIPVDGIVREGATSVDESLLTG
                     EAMPVDKQSGDAVHGGTINGSGSIVMEVSSAASESALARIATMVAEAQGSKAPLQRLA
                     DRVSSRFVPAILLISALTGIGWWFLSDVSADKAIMVAISVLVIACPCALGLATPTAVM
                     VGVAKAARGGVLVRDAASLERCAAVDTIVMDKTGTLTEGRPTVTSVHAIDGVDKQEVL
                     RYAAALGSNSEHPLGKAAVQAARDADLSIPSPTDVTAVPGQGVQGIVQEKSVRMVAAG
                     DPSDVLPEEVMKARSQGGTVSLVEVEGQALGIVVFHDQVRPEAAAAVRELQAMGVQVL
                     LATGDHEAAGRAVAESVGIAGQDVHARQRPEDKLALVREKKAAGRVVGMAGDGVNDAP
                     ALAQADVGMAMGGGTDVANEAASMTLARGDVRGIAHAVRTARGTVRTIKQNLGWALGY
                     NLLAVPVAAAGLLEHLGGPIIAAAAMALSSVSVVLNSLRLGRK"
     gene            complement(11509..12480)
                     /locus_tag="PK710_05765"
     CDS             complement(11509..12480)
                     /locus_tag="PK710_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09255.1"
                     /translation="MSELDGPALAFMQDLTIRFDMNLVFATPRKPPRGASLHGRVAVL
                     DLAFVSDAISSGFERITLSFIEELGPRLAVWVDHHDHEQHARFASDPRFVLCTKAEHG
                     ACPELITPALVERIGPVDTLVCHTDFDGLASAAKWIRKGIEPYEGCDADARIIDTRIG
                     NPSSYAEVMDRALRARPNDHALFGLMVRHLAQGLKDASLWIPIREAAEQLRAIEAETR
                     KLALAYVRLAPGVAFVDVSHRRARIDKTLLLLLGQEREPIAMVLDRDTINLATRFDGG
                     VNLLKLLGLSGGMPTRVSVPRKRLERTCEALGVDWSAVEPLLVAYHP"
     gene            12500..13558
                     /locus_tag="PK710_05770"
     CDS             12500..13558
                     /locus_tag="PK710_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09256.1"
                     /translation="MMAVERRSVIAAMACFAGLFHSMSAAYADQSGQEVNADGSGSSC
                     VISGDAIVVKDTPIFSEAVGGNPVAKFSGAKAPLKATRCPSDSSTGRVLINTGAGFRV
                     EGFTDPRALKAFGIRDLPVSGSLWISASAPLTIVGSAPGKVQVEHPAMAGLSRPIRAW
                     APCDAITFTQGSAPIYEVPKNARGYVARQGALDIYASAGGDVAHTLSISGEGNGLLLW
                     GTTLRAGFVHVTMRSSIFIDGWVKTSEVRALPRGELMDQLAPPTQVQLPPALALKEYL
                     SVMRAPKPLSLYYGRGEANPTMGMVEQDAEIYVLETVVGWATVLPKSLHVLPVSDRAF
                     WVKATELGIGAKPDGGGP"
     gene            14027..>14226
                     /locus_tag="PK710_05775"
     CDS             14027..>14226
                     /locus_tag="PK710_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09257.1"
                     /translation="MQNIFFGKLHGTSGQCFDVGGSRAQRIELSVADLESMFQQVSAH
                     WLEKLTLFDISNDSTEIPFGLAV"
BASE COUNT         2959 a         3965 c         4164 g         3138 t
ORIGIN      
        1 tccaactcca accgttctgg agcacgcatc cctttgcata cgttgccatc cacgtattgc
       61 tccaacacgt cggccacgcg aaaaatcgcc tcttgcacat ccgccgccga catcgaggca
      121 tccttgatga cccaatcgtt ttcgctcgtg accaactgac tcaactccga cagcgccttt
      181 cgaagctcca aggattgttc gagcgtgttc ttatacgaag gtacgagatc ggacctttcc
      241 acattcacgc cgaagatctc gaacacggtg gacgccatcg cgaagatccc gtcgcccaaa
      301 gcctgacggc tcgcatcgag ttcgcgcagc gcaagctcga agttacgacg ttcgatggcg
      361 atcgcgattc gcaaatcgaa tgtttctgct tgctgtgtga gtacgccgcc gagcgttccc
      421 agttgctcac gaaacaaccc caacatggtc gatgaatctg gaatctcgtc cgcaatggct
      481 tcgaaagcct tgtcaatctc atcgatttca agatcctcga gcgtgggcgc gcctgccggt
      541 gatgcctcct cgaaacacaa atcgaaatcg tcaccgaagt cgaagccttg gttgtccagc
      601 gtggaaggtc gctcggagtg tcgaagcatt cccgaggcat gcagatagga acatagtttg
      661 cgaatagcgc ggaaggtctt cgcatgcacc tctacgaacg tggcttccgt gatccccgcc
      721 tgcaattcat catgatcggt caacgatagc ttcgcattcg caaagtagtc gtacagcgtc
      781 tggtccagct ccgcgagctg ttccatcgcc atgatacaca tctgcaagac atccgatgcc
      841 acggttcgct gcctgtgatc ggagaccaca tcccgaacgc gcttgatcaa tgaacgggca
      901 tgttccttca tgacatcaaa gcagttttct catgcggtct tgtttgccct ttgccgcgaa
      961 caagggtcca aggcccttca tcgtctcacg aatcctcgac atggcagcgc ttcattacct
     1021 gcgccggtac tcggcctcca taatcgtatc ccaatccgca tcatcccgct ggctctgcaa
     1081 ccgaaacaca taggctccgg gttcgaggaa tgtgtgcgaa tagcgcactt tcccgccagg
     1141 ttgctcgagt acaaacacaa gctcgttttc cttccacgtc ccacgggctg gctgcctcgg
     1201 ggcaagcccc atggaatcga accaatataa tacgtactcc ttctcgatat cgtcccagcc
     1261 atacacccca tgccccacga acgtctcccc cccatccctt tgttccacat aatcggatac
     1321 caaaaaaaag ccgtccgcca ccaccgtgtt cgaaaaacgt cccacagcca tccccgccga
     1381 accccatgcc gatggcgcca gccgcacgat gccttcccaa ttgcctacca gggtatgaag
     1441 cttctcatgc tgttttcctg gttgcggtct ccagttcatc gatacaacct cgcttatcct
     1501 cgtggcttcc agttgaccgg cagcctgtcc tcccatgata gaaccaatcg catggcggta
     1561 gacgatcctt ctaactgtcg acccaatcgg tgccgtatcg caacagccac acggttcggg
     1621 tcatggtttt cgtgcttcat cgccctcggt tgctccgcgg cccccgttac ctcaccgcaa
     1681 aatccacaag ccaccttggc actgcccgag gctgcgaaaa ccctttctct gggacgacgg
     1741 caggtggctg ttgccgctag tccgactcct ctgctcgaac gcctggcttc tgaagtccag
     1801 caattcggaa cggtattttc ttctcgcgaa gatacgcccg tataccacct ggcctacgag
     1861 gtgaacgaca cctcgaaagt cgatatcctt gcgagttacg gagccattct gagccacacg
     1921 catacacgtc gacgcttgct cgatgtggac ctacgcgtgg gttcctacga actcgacaat
     1981 acgcatccgc tgattgattc cgcttattat caaggcaatg cggcgatcgc ctgggacgac
     2041 gatgctcatg aatccctgca taacaccctc tggtgggaaa ccaaccaagc ctataaaaaa
     2101 gcttgtaagg agcttatcga agtacgcgca caacgacaga tcgacgtggc aagcgacgat
     2161 caatccagcg attattccga agaagcaccg cttgtccacc tcgaagctcc cgttgcactg
     2221 actgtcgatc gcgcggattg ggaacagcgc gttcgatcgt tgagcgcctt gtttcgggga
     2281 catgaacaca ttcaccaggg cttcgttcgt ttcgaactga cgaccacgaa ccgctcctac
     2341 gtgagcatgg ggggacatcg cgtgcagacc ggaacaaccc acgctcgggt gtcctttgga
     2401 gcgtcgggca tcagcgatga cggtgacgaa ctcactcgtt cggatgagat cgatgcgcac
     2461 gacgtttcgg gattgccgaa cgatgagcat cttcgttttc gagttcaaca catcatcgag
     2521 gatatggaag gcctggcgaa agccccgaaa gcagagccct acgtgggccc ggcccttgtc
     2581 gatggcaaag ccgcggccgt gctatttcat gaggtctttg ggcatcgcgt ggaggggcat
     2641 agacagaaag ggcagatcga aggacaaacc ttcatcgatc aagtggggca acccatcatg
     2701 aattccgtgt tcgatgtgta cgacgacccc accgtaagtt ccgtcaacgg catcgatctc
     2761 aatgggtttt atcgcgtgga ttccgaaggg gttcgagcgc gacgggctca actcatcgaa
     2821 aagggtgtat tcgttggttt tctcatgtct cgaatgccca ttcgcggcat ggcgcactcc
     2881 aacggccacg gtcgtcgtga accaggctat cgagcgatcg cgcgacaggc caacttgatc
     2941 gtcgatccat cgcgggtcac ctcgagagaa gcgctggagc gtgcattgat cgatgaggtg
     3001 aaacgtcagc gcaaaccttt cgggctgcgc atcggtgaag tcacgggtgg ttatacgacg
     3061 acccagcggg gagatccgca agccttcgtg gtcgaaccgg tgatggtata tcgcgtatat
     3121 cccgatggac gacaggagtt ggtccgcggc atcacgttgg aaggcacgcc gctttccctg
     3181 ctgagtcatg tgctcgcggc gggtaacgac ttcgcggtgt tcaacgggtt ctgcggggcg
     3241 gagtccgggg aggtccctgc ttccgccatc agtccaagct tgttgctacg acaaatcgaa
     3301 gtgtccagac agcccgtcag cggtcaacgt ccaccgctgc ttcccgctcc gcagatcgct
     3361 cgttcgtcag ggaggaagcc atgaaacagc gattcatcaa ggcgctacga atcgctgccg
     3421 caggtatcgg caccgttgcg attcccatca tttcccacgc gcaaatccct tcgcatccca
     3481 tcagcgtttc gccatgggtc tcaccgatgc aggatgaaat cgagcgtgcc atgtcgctcg
     3541 aacttccagg catgcctcgt ccccactaca tcatcgcaac cctcatcgaa caacaagaag
     3601 cgctcgtcga agccagcctt ggagcggtcc tccaacagca ccaggatcgt tccagggttt
     3661 tgaaagtcga agttcgcgtg ggcgatcgct ccttcgacaa cagcaatttc cttggaggct
     3721 caccgcaagc catcgtggtt tggcccgctc cgctcgatga cgatccgctc tgtttacgac
     3781 gcttgctttg gcttgccgcg gaccgttcct acatcggggc gactgaatct tatgaagcca
     3841 aacgggcgca tcgggaggcg atgacgatcg aacaggaccg catcgatgat ttctctgcgt
     3901 ctgcagccgt gagatttcgt ggttcatccg ctcccgttgt tccgccattg gatcggctca
     3961 gcgcgttggc cacggcggca tcgtctgttt tttccaatca tcccaaggtg tatcaatcgc
     4021 gcgttcgaat ggcggtacgc tcccaagcgc gaaccctgct tgattcggaa aatcgggtca
     4081 tcgaagacag cgcgtcgctt gtcgaaatcg aaatcgttgc gcaaacgcaa gctcgagatg
     4141 ggatggaatt atccgatcgg catgcattcg cttccattca tttcgatgag atacctaccg
     4201 aagagaccat ggttcgttgg gcgcgagaac tgatggttcg gctcgaaacg atgcgcgagg
     4261 ctgccatcgt tcaaccctat tcggggccgg tattgttcga gggaagggct tcttcgcgac
     4321 tatttcgaac cctgttggcc gaggaattat ccgcaactcc atcgccggag cccaccgagg
     4381 gtcaggatac attgggcgct ttggccacac gaaaaggctt tcgtattttg ccggtaacct
     4441 tcgatgtcgt ggatgatccg acagtggatc gaatcgcggg tcttccggcc atcggccatt
     4501 acctattcga tgatcaagga gtgcgggccc aacccgttca cgtggtgaaa gaaggcattc
     4561 tcgacatgct gttgtcgtcg agaacgccat cgaagacggt tgctatttcg aatggtcatg
     4621 ggcgggctgg ttggctgggc gacgctcgcg gacgagcagc gaacgtatgg attcgagcgc
     4681 gtgatggtct gtctcgcgca tcgttgcgaa acgcattgct cgaggccgcg aggaaagaag
     4741 gtttggacta cggaatcatc gttgcgggtt ttgaagaatc gtctggaatc gaagggactt
     4801 ctttttcgct tggacgacga gacagccaat ggccgccgtc ggcggaggcc tatcgactgt
     4861 accggggcgg gaagctggaa ccgatacggg ggttgacgat ccaggggctt cgtcctcggg
     4921 atcttcgtcg tatcgtagcg gcgggggcgg atgcgaatgt gtattcgtat cgcgcctcgg
     4981 cgaacgccgt aagggcattc aacgacagcg tgggagagat acctacaacg atcatagctc
     5041 cttcggtatt atttcctgat gtcgatgtgg tgggaatggc tcctccctat cctttggttc
     5101 ctgctgcggc gccttgagga aaaaatatcg acgtgtctgg gaacaagatc gccccatcga
     5161 tgtcttggtt cgatacgcct gtttgaaagg agccgaggat gcgagcatgg gtggttagtg
     5221 cggtgatgtt gatgggggct tacggacttt ggatgtcctc ggggattcat cgagaggcgc
     5281 ctttggttga gcatcggggc atgacagcgg gggtagtggc gatggaaaat gaggtggccc
     5341 tggctccgga cgatgcgcag aagctgtcat cgttgtgtca ggcttatctt cagcgaaacg
     5401 cgccagggct tgctctggcg gcgattcatc gcgcgccttc gatggtccag caacagccgg
     5461 agattcagca tctttgggcg aaggcgttgt tatatgaagg gcaagcttct gaggcgctcg
     5521 acaagcagcg tttcgttcta gccgcgtgtg agaagcagga gtgttcggcg tggctggtag
     5581 cgtcggccgc gaggcaggaa gcgtttttgt cggcccttgt cgatggggga gtcgaggacg
     5641 tatttcgcaa tccggggcaa gcgtttgagg cgtatcgttt gatcagtggc cctatggtga
     5701 cggtgatgga tagcgagcgt cagacggtcc agtgacacga caggcggtgg cgggtggatg
     5761 gtggggagag ccgctagcga tgggttgtgg cattactctt ctggttctgc gggttcgtgg
     5821 tcgtgggcgt gtttgttgtt ggtagcttcg agccattcga aagccatatc gacgacttcg
     5881 acgcgcgtgg agggagcgac atattcatcg tcggagacaa cgggcagggc ttggagtctg
     5941 cgcgtgaggg cttgccgcaa agggggggcg aagcggaata cctcccattg tttttcagct
     6001 tttgggccta cgagggcgac atgggccatg gcgacgggga aaatcgtgat ggaaggggcc
     6061 gaaagggacc agagcaactg gtcttgggcg aggggttccc agcagagtgg gtggcgtgcg
     6121 cgcagcaatt ccccgaggta gcagccggct accgaggcga tgagggcgtg ggtatggggt
     6181 gaaccttgag cgcgggcctc gcgcagatag tgatccagga caggaagggt ttcggtctga
     6241 tagtcaagag caataccgag attcttttgc acgtattgga cacacgtttg cgcaccatcg
     6301 acaacctcga tgggtacggg cgtattcgtt gatgaacggt cccaacttga ggtatttccc
     6361 atggtggtcc accatgcttc cggatgggta aagacgcaat ggtcgagcgg gcggaccgaa
     6421 ctgacggcaa tggacattcc tatccatcac ttttccggca cggcgacaca cctgtgctag
     6481 gatggcaaac cttgtaggcg atgcgcattg caatattttc agacgtccac gcgaactacg
     6541 aagccctcag tgcggttatc gaggcctaca agcgtgagga gatcgacgtt tactactgtt
     6601 tgggagacac cgtgggctac ggtggttcac cgaatcattg tgcggagttg gtgcgcaagc
     6661 ttgcgtccaa aacgatttta ggcaaccacg atgcggcggt agctgggcga atggactatt
     6721 cgtattacta cgaagctgct cgcaatgctt tggacacgca tgcggcgatg ctcagcggtg
     6781 aaagcatgag ctggctcaag aacctgccgt atcaggaacg cttgatcgat caggacttgc
     6841 agctttgcca tggttcgccg attcgtctcg aggagttcga gtatattttc gcgccggaac
     6901 aggctcgcga gtgcttgtcc atctgggacg aattgcagca ccttacggtg attggtcact
     6961 ctcatctgtg caaggtattt gctctcgacc aggattcggt cgaagagcta cccgccaccg
     7021 acttggtgct cgacccccat cgcaaataca tcatcagcgt cggttccgtg ggacaaccga
     7081 gggattacga caaccgtgcc agttacaccg tgtacgacct ggccacgggg cgattcgagt
     7141 tcaagcgcat cgaatacgac atcgaaacgg cggcggacaa ggtattgaaa gcggatttgg
     7201 agcgcaactt cgcgcaccgg ctgtacatcg gcgtttgatc ctctcgatcg tcgtttcgtc
     7261 actcatccag cttgaccggt agaacccctt ttgccatggt gcgccgcgga gagatgaccg
     7321 agaggccgaa ggtgcctgat tcgaaatcag gtgtgcccgt gagggtaccg cgggttcgaa
     7381 tcccgctctc tccgccagtg actatcagca aaaagtgagt taggagttgg ttggctggtg
     7441 ttccagccat ggttcgcttg ggagagatga ccgagaggcc gaaggtgcac gactggaaat
     7501 cgtgtgtacg gaaacgtacc gtgggttcga atcccactct ctccgccctg cacgccgcgt
     7561 gacgcaaacg cggcgttttt catttcacac ccttgctttt gaggctcgcg gttctcctcg
     7621 catcgaccct acggaagcgg taggctcgac cgcaatgcgt gtgcgttggc tcattccatg
     7681 gcttgccatc tttgcttgcg gaggaccgcc gtcggccacg actcccagtt cgtccacgcc
     7741 cgcgacagag cgtcccagcg acctgacgag cgatgaactc cgcagttacg atgcttacct
     7801 cgagcgattt cgctcgccct gcgacaacaa ccagtcgttg gcgtcgtgct tgtcgtctgc
     7861 tcggtcgtgc tcctcttgcc gtgccgccgc gcaattcgta gctcgttcca ttcgttcggg
     7921 atatacggcc aagcaagtgc aagcgcgcta tcaggctcgc ttcgatcccg ctctcgttca
     7981 gatcatcgat gtgcggggag cccccagtct cggtcctgac gatgcccctg tcacgatcgt
     8041 cgaattcgcg gacttccaat gtcctttctg tgcggtttcc gctcctttgc tcgatgcgct
     8101 cgtcgaattg tacacgcctc atttgcgcct cgttttcaag gactttccca tcaagtacca
     8161 tcccaatgcg ctatccactg cccaagcggg agtggcagcg cataaccaag gcaagttctg
     8221 gcaattgcat catctgatgt tcgccaaccg tgaacagctc gatccaccgg acatcgagcg
     8281 gtacgcccgc agtctcaacc tcgacatgca gcgctttgtt tcggattggg agtctgcagc
     8341 gacaacccaa cgagtcgacg ccgactacca ggagggaatc aggctcaatg tccgaggaac
     8401 gccatcgttt ttcatcaacg gtcgcagctt cgacatcgaa cttttcgatt ttggtggtga
     8461 agacttgttg gcgtggatcg aactcgaaat cgagttgacg accggaaaac catatcaaaa
     8521 gtaggagtcg ttcatgcgag gtgtctccga tgtctgcggc tgctttccca cgggtcttcc
     8581 ctaccctgga attgcgcaac tcgttcgcac caccttgctg ggagtcgtcc aagaactcga
     8641 tgcatccctg ccgtgggtcg cccatcccat cgtcatgatc gataccgaaa cgacaggtcg
     8701 taaggcatcg gaagatcgga tcatcgaaat cgccattgtc aaaggccgtg atggcgagat
     8761 tctcagtcgc gattcctggc tgatcaatcc aggatgcccc atcccggagg actcatcagc
     8821 cgttcacgga attttggacg aacacgtcga aagcaagcca ttttttgcgg atgtctgcga
     8881 cgaaatcatc gaaaaactcg agggagccat tcctgccgcc tacaacgcgt cgttcgatcg
     8941 cgagtttctt ctcgcggaaa tccagcgttt gcaacgtcat gatctacgcg acgtgccagc
     9001 catacgcgat cgcgtcgttt ggatcgatcc gctcgtttgg gcccgtcacc tatttcaaga
     9061 agaaaaatct cgtaaattat cggcgatggc cgaactgctt ggcgtcgaaa tggaacgagc
     9121 gcaccgggcc acggatgatg ccagcgccgc tttgcgtctg atgtacaaga tggccgagca
     9181 aaaccgtgtt ccctcggcgt atggcgaatt cattcaagag caagttcgcc tgtcgcgcat
     9241 ccagggagaa gcgcgcagtg gatggcgcag gtcttgagcc cattcgcaac gaacaccatc
     9301 gattcgcatc caatacagtc atgaatcaag atccgtcagc gactcccacg ctgactcgct
     9361 acacgcttcg agtcgaggga atgacatgcg ccgcatgctc ctctcgcgtt caacgtaagc
     9421 tcgacaaact ccaagggatt gaatcggcat ctgtcaatct tgcgacacac ctggctaccg
     9481 tcatggccaa gccagaattg ggagaggagc agatccgcgc atgcgttcaa gcagccggat
     9541 acggtgtgga agtcgtgcgg cacgaggata ccgccgcggc tttggcgcaa cgaccggatg
     9601 accgccctgc ccttcgtcga ctcattgtcg ctggcgcgct cgccgctccc ctcatggtcc
     9661 ttggcatggc gcatgtgaat gcgccttggt ctttcgcttt gcaagccatc cttgctttcg
     9721 cggtcaccta tggagcgggt tggaccattc attccgctgc cgttcggcgt gcgctgcatc
     9781 tggataccac gatggatacc ctcgtgagtc tcggttccac cgctgcctat gtctatagct
     9841 tggccgcgtg gttatcctcc gattttcacc atgtatactt cgaaaccgcg ggaggcatcg
     9901 tcgcgttcat tctcgtcggt cgatatctcg aagcccggtc caaagctcgc gccaccgact
     9961 cgcttcgctc tttatttgcc atgcggccga cgcgggccac actgcttcgc ggcgacggtg
    10021 aggcggtcgt tcccatcgaa gtcgttcgtg tgggcgatcg agtccttgtc aaaccaggag
    10081 aacgcattcc ggtcgacggc atcgttcgcg agggcgcaac atcggtcgat gaatcgttat
    10141 tgacggggga agcgatgccc gttgacaaac agtcgggcga tgcagtgcat ggcggtacga
    10201 tcaacggttc gggttcgatc gtgatggagg tatcgtctgc agcatccgaa tcggcgctgg
    10261 ctcgcattgc gaccatggtg gcggaagcac aaggctcgaa agctcctttg caacgtctcg
    10321 ccgatcgcgt atcgagccgg ttcgttcctg cgattttgct catctcggca cttaccggaa
    10381 ttggatggtg gtttttgtct gacgtatcgg cggacaaagc catcatggtt gcgatttcgg
    10441 tgctggtcat tgcctgtcct tgtgcgcttg gactggcaac gccaacggcg gtgatggtgg
    10501 gtgtggcgaa agcagcgcgg ggaggcgttc tcgttcgcga cgccgcgagt ttggaacgat
    10561 gtgccgccgt tgataccatc gtgatggata agacggggac attgacagaa ggccgcccga
    10621 cggtgacatc ggtgcatgcg atagacgggg tcgacaagca agaggttctt cgatacgcgg
    10681 cagcgctggg ctccaactcc gagcatccgc tgggaaaagc ggctgtgcaa gcggctcgcg
    10741 acgcggatct atctattcct tcccccacgg acgtaaccgc ggtgcccgga caaggggtac
    10801 aaggaatcgt gcaggagaag tccgtgcgca tggtagccgc tggagatccg tcggatgtat
    10861 tacccgagga agtgatgaaa gcgcgctcgc aaggcggcac ggtttccttg gtcgaagtcg
    10921 aaggacaagc gcttggtatc gtcgtgtttc atgatcaggt acggccggag gccgcggcgg
    10981 cggtgcgaga actacaggcg atgggtgtgc aggtgttgtt agccaccgga gatcatgaag
    11041 cggcgggcag ggccgttgcc gagtcggtgg ggatcgcagg gcaagacgtc catgcgcgac
    11101 aacgaccgga agacaaattg gctctggttc gtgagaaaaa agccgcgggg cgggtcgtgg
    11161 gcatggctgg agacggtgtg aatgatgcgc cagcgcttgc gcaagcggat gtcggtatgg
    11221 cgatgggagg aggaacggat gtggccaacg aagccgcgtc gatgacgttg gcccgagggg
    11281 acgttcgcgg cattgctcac gcggttcgta ccgcgcgcgg cacggtgcga acgatcaaac
    11341 agaacctcgg atgggcgctt ggatacaatt tgcttgccgt tccggtggcc gctgccggat
    11401 tgctcgagca cctgggtggc cccataatcg ccgcggcggc catggccctt tccagcgttt
    11461 cggtcgtcct caattccctg cgattagggc ggaaatgagc ttgaacgctc atggatgata
    11521 ggcaacgagc agcggttcga cagcagacca atcgacaccg agcgcctcac acgttcgttc
    11581 gagtcgcttt cgtggaacgg aaacacgcgt gggcatacca ccagacaatc ccagtagttt
    11641 gagcaagttc actccgccgt cgaaacgggt agccagattg atggtatccc gatccagcac
    11701 catggcgatg ggctctcgct cttgtccaag cagcaataga agtgttttat cgatgcgtgc
    11761 gcgtcgatgg gacacatcca cgaaagcgac gccaggcgcc aggcgcacgt aagcgagggc
    11821 aagttttcgg gtttccgcct cgatggcgcg gagctgttcg gctgcttcac gaatgggaat
    11881 ccacaaggaa gcgtctttca agccttgtgc gagatgtctc accatcaagc cgaacaaggc
    11941 gtggtcattg ggtctggcgc gcagggctcg atccatcact tcggcataag aagagggatt
    12001 tcctattcgt gtatcgatga ttctcgcatc ggcatcgcat ccttcataag gttcgatgcc
    12061 tttgcgaatc catttggcag cgctcgccaa cccatcgaaa tcggtatgac aaacgagggt
    12121 gtcgacggga ccgattcgct cgaccaatgc tggtgtgatg agttccgggc aggctccatg
    12181 ttcggctttc gtacacaata cgaagcgagg gtccgacgca aatcgcgcgt gctgctcgtg
    12241 atcgtggtga tcgacccaaa cggccaatct cggacccaat tcctcgatga acgacagggt
    12301 aattcgctcg aagccggaac tgattgcatc ggaaacgaaa gcgagatcga gtacggcaac
    12361 ccttccgtgg aggctcgccc ctcgcggagg tttgcgcggt gtggcaaata caagattcat
    12421 atcgaatcga attgtgagat cttgcatgaa agcaagcgcc ggtccgtcca attcggacat
    12481 gagcgcacgg aggtctagca tgatggctgt tgaacgacga agcgttatcg cggcgatggc
    12541 atgtttcgcg ggcctgtttc attcgatgag cgcggcctac gccgaccaat ccggccaaga
    12601 agtgaatgca gatggtagcg gcagctcgtg tgtgatttct ggcgatgcga ttgtcgtcaa
    12661 agacacgccc attttcagcg aggcggtggg aggcaatccg gtagccaagt tttcaggggc
    12721 aaaggcgccg ttgaaagcta cgcgatgtcc cagcgattca tcgactgggc gcgtgctcat
    12781 caacacgggt gccgggtttc gggtggaggg gttcacggac cctcgtgcat tgaaagcctt
    12841 tggaattcgg gatcttcccg tatcggggag tctttggatc tcggcttccg cgcccctgac
    12901 gatcgtgggt tcggccccgg gaaaggtgca ggtcgaacac cctgcgatgg ccgggttgtc
    12961 tcgtcccatc cgcgcatggg cgccttgcga tgccattact ttcacgcagg gttcagctcc
    13021 gatttacgag gttccgaaga atgcgcgggg gtatgtcgcg agacaaggag cactcgacat
    13081 ctacgcttcg gcagggggag atgtggctca taccctttct atttcgggag aaggcaatgg
    13141 gctattgttg tggggcacaa cgcttcgtgc gggattcgtt catgtgacga tgcgctcgag
    13201 catatttatc gatgggtggg tgaaaacaag cgaggttcgt gcgttgcctc gcggtgaatt
    13261 gatggatcag ctcgcccctc ccacgcaggt gcaactgccg ccggcacttg ctttgaagga
    13321 atatctaagt gtgatgcgag cgcccaagcc gctttccttg tactacggca ggggagaggc
    13381 caatcccacg atgggaatgg tcgaacaaga cgcggagatt tacgtgttgg agacggtggt
    13441 ggggtgggcg acggtgttgc cgaagagttt gcatgtgttg cccgtttccg accgtgcatt
    13501 ttgggttaaa gcgacggagt tagggatagg tgccaagcct gatgggggcg gtccatgaca
    13561 gatccccaca ttttttcttg aattggaaag ggattcgagc aggggattcg ctggactttg
    13621 gctttgacct ggtccttgag tccatttgga tcacgcctta caaaaacacc cattgccctt
    13681 gtcaatcacc atccaaccaa taatctccag acatcggcag gcggcctgac gccacggcca
    13741 cagatcaatc gccgtaccca tttttataaa accggtaatg accatggctg tgcataagcg
    13801 cggcggccac cgcgctgccc aatcacccag ccaccggcac agccgcagcc atcagatcag
    13861 ctaaaaaacg ctcgcgacgt acaatatcga ctcgaaacga tgcccctatc atccatcgca
    13921 aatgggtaca tacggctctg acttcagccc aagcaatacc cccaatggca tccaaggctc
    13981 ggcacgacca gaagcaaaac gctaggacgc tgtcggattg caaagcttgc aaaacatatt
    14041 cttcggtaag ctgcacggca ccagtggcca gtgcttcgat gtcggaggct cgcgcgcgca
    14101 acgcatcgag ctatctgtcg cggacctcga gtcgatgttc cagcaggttt ctgcccattg
    14161 gctcgagaaa ctcacgctgt tcgacatttc gaatgattcc accgagatac cgttcgggct
    14221 ggcagt
//
LOCUS       DAOSOR010000071        14195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4312_length_14195_cov_4.343918, whole genome shotgun sequence.
ACCESSION   DAOSOR010000071 DAOSOR010000000
VERSION     DAOSOR010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 14195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14195
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4312_length_14195_cov_4.343918"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2912)
                     /locus_tag="PK710_05780"
     CDS             complement(<1..2912)
                     /locus_tag="PK710_05780"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT09258.1"
                     /translation="MSTRIFHVTRWLWLTLLVVFASCKKDGTIEASVADLQVVHEGVS
                     LNGSDRLQTHRVGDAGVIETKPEGRARLRLDDGTVAIVDGNTKVTLTAGTLRLERGRL
                     FLVGAIGARTTIELGEASAIMTGGHAGIERDAQDTKKASVYAASGELVVQAAGKDHTV
                     QTGETASIEGTVVKVAPARVFDDWTGGLVAPWSATGSMRRAVGTLWGSSGATVGDPGS
                     PLTLRSQNVAATVIGETASTVVHSTFFHAGSEPVWGDFRMALPAGAIVSGFAAGVGDS
                     LREGRIGLKDTNFSATTSEPTLEWAGDGWVRGTLRGISPGSVVHVVVRYAQWLPRRKT
                     NDGVVVEYRFPLASDAEPPLIGEFSVQVDTTVTPPRSIRCGHGATVENGVVMLRKSDF
                     RPSADFVVELEMAPDKSPARLYMAEGDDDDEAGSYVLVRAEAPEPERKRGARLAIVLD
                     TSSSMDAGAFDAARNFVETLVRSLGPNDQAVVLGANIAAQSVGPEAIGAMDEARQKAV
                     ITALGGLEPAGASDLGLAIEAAADRLDPKDPAGLVVYVGDGWPSVGDLQAREIRARLA
                     RRTGGSPRLGAISVGSRSNRFGLMALVRGLGPVLHVENANEAPEAAHQLLADALQPTV
                     ASVAIDLGPHVEQVYPWNAQAVVAGTTVFAVGRTREQAPREVTLRWRGENGEETKKLL
                     VKRERIADGTDVRKRWALARVEELALRSRGREAVTDVALREKLMTPWTGWTLNTGERP
                     RYPELSMVERLLDLPMEQDAIFSAEFATPQELAPTMLDLSSEPMGNVGMGQAGFEMAV
                     RMAAKRTLDAAIGGIRACRDSRAALRPDLTGVLDVSFQVDGNGGASDVHVEGSRSAYD
                     PALFSCVASVISGLSFPASGLTTKIKVTHTIQLPPAKPTGRTKCSTTSQLPLQARRGA
                     WQQRLGGTSAYDVFLEAKLQCEVRDWTAKRSLLELIMLHVSGENRVTLARQLEVVG"
     gene            complement(2909..7543)
                     /locus_tag="PK710_05785"
     CDS             complement(2909..7543)
                     /locus_tag="PK710_05785"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HOT09259.1"
                     /translation="MPSCSRIVHSVLVVFLLLIIGSCGPKQSLAPQSQTFGEIRAIRG
                     AITVAPAGASSRSILPRERLTDGSTIEVAPNALAWLRRDGGSTVLLRGPATVVFGVDD
                     IHVKKGQVFIDSPDDQVTTITSAVGSLYLSRVRASLEIGEETEAYVLSGEVRTPDGIR
                     AGPGERMSLARDRKPLVQPALAWVDWTGGLATTDRGAEPAPFGVGTVGARLPGEQGQP
                     RTPLAIRRMEVRVSVEGDLAITEVDQMFFNGASQTVEGVYTFRVPIGASLTRFGVDRE
                     GVIVWGRVKEKMAAAAQYASHVYEGSKEDPALLEWDSPGVYRARLYPIAAGATRRVVV
                     RYTQWLGRTGSRGERRLYTFPMAAEGAEETLPHIEFFGATFDLGRAGAKEVRTGMQGV
                     IEKDKVVIRAHDLVPRADLAVELFDDGAKGSQAYLAPHTVDFELVAPTDRAEARKRAI
                     EEASYLAIPIRASDIPKQEGGLDLVMVVDSSAGMDNASMVVTRAAVHALLSHLGEQDR
                     ALLVAGDTTLRPVVRGWDKLRPVTEAVRKDASIGLATLERGGATDLGAMIAQAAASLD
                     PARRGVLIYIGDGKPTVGELALVDLKERLAKLPRPVRTFGLGVGQDADMSIIEGLVEA
                     GFAERLDDAADAARTALRLLEEAERPAWLGVNVDLGPAVERLVPRELKTLIADESVIV
                     VGRLTGDPPKTIRITTPAGDNTLAISPQRLADFGDLQARWSTGRLRQLLADGVGRAAL
                     VDLGVRGGIITPYTSLYVPTTQEMSSDELEQLRKNQRNLDKNPRGALLPTEPESKYAW
                     LNSLLLAGCQSKSEKTATMEAAPLAEVAPGDIPETVKLEQQQDYAEKKAEEGSGRRKS
                     STFAARGPTDSSGETAKVSALSEKDRGGAPSPPPQAQPAPLSTTTAEAVAGSAIQTGS
                     DNDDKTADSMLGIMNIGAGGGGKGEGIGQGFGSGHGRLGGSHQSQSGKVKMDVADATG
                     PLPRPVIERIFRQNYGAFRMCYETGLQSNPNLRGRINLRFDIGADGSLSNIRDADTGF
                     PDAGVVSCVLRACERLSFPASSGPVAATYTITFSPPEGLVKPSQPLNIHQFIVNLNLP
                     HQVVPCAQGSKLPLADRVALWRERLGNARGEPQAVASVYHQAINQCEASAWPERSRLL
                     SMMLDALPTVEKRVALWRLMQAQRSVADSLYRGILSRIRKTDEMRQLHQALGLRSVDP
                     GLLEELMKKEPNPAARAVMLRDLRLQWPDDSTLSLALLHALEDANDDSAARVLGRQLR
                     NRPDADAHVLTEVGELYLRLAKRSASKELASEDEAEARRAFGEIVEYSPDEPVARRRL
                     GDLLRSHGWFAEAARQYETLARLAPDETGVFLLQASAAQGLGKLDEAVRWAEKASNDG
                     APGGDAQGLARTARSLAAVFLAWGRVQAAAENRNDEFELLRQRTMRLLSQDDRSENTA
                     RAVLVWSHPELHPTLWSNALGSMMPAPEGDMTLGLSQVILPRRDGMVLEIRIEKRDLT
                     NIARLGAQALLTVIFQEGKADEKVVRVPVVFNPEGPAALRFRVDGMEVKP"
     gene            complement(7731..8096)
                     /locus_tag="PK710_05790"
     CDS             complement(7731..8096)
                     /locus_tag="PK710_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOT09260.1"
                     /translation="MEPWIVPLFVVIALLLLWKMAGAYRSPKQLQAIAEALRSGATLI
                     DVRSEAEFASGHLPGAIHIPVNDLHPERLKDKTRPVVVYCASGARSAYAAKTLRKAGF
                     TALDLGPMFNGRKVLPQSP"
     gene            8224..8619
                     /locus_tag="PK710_05795"
     CDS             8224..8619
                     /locus_tag="PK710_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09261.1"
                     /translation="MAQRPGTMDGYPCFGPGQRDKARIEQSAPEAVVKLLVGVGAGRR
                     YAESLVEEGIFGKSDFAKAHPIRDTMPFSARLDEAAMGPCRGKVCFDDGVGLGEQGLL
                     QRSGDRTRSEFVGEEGKVSHASLLAVVDE"
     gene            8966..10594
                     /locus_tag="PK710_05800"
     CDS             8966..10594
                     /locus_tag="PK710_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOT09262.1"
                     /translation="MANRHQAFGGNYGKHREFSWGSDGTTFDRAGDMLATAHTTVLLG
                     MNAQPVRVEVESFRGVPKFELVGLPEASVRESYVRVRSALNQLGLNIGQQRLVVNLAP
                     ADLRKPGSAFDLAIASAALAALGHVNRESLHDTLLLGELSLHGAVRPVRGVLPQLMAA
                     RNNGMRHAIVPAGNGREASAVDGIDVKVADTLHDVREHLRGQSPLPSPQAVVFAPSFD
                     ERLDLIDVKGQPGARRALEIAAAGGHHLLMIGPPGGGKTMLARRLPSIMPPLTYSEAL
                     EVTAIHSVAGLLPSDSGLVTIRPFRAPHHTVSDAGLVGGGAPPRPGEVSLAHQGVLFL
                     DELAEFRRSAIEALRQPLEDGQLTIARAKERATYPARPMLVAAVNPCPCGYAGDSSGR
                     CQCGEHRIHAYRAKLSGPLLDRIDLHVHVPPVDVTFLRERPCGESSATVRARVMAARA
                     IQARRLETGQVESRCNGALSSRELDAVALPDAQGMKLLIAAAERLGLSARAYGKILRL
                     ARTIADLESSPDVRSVHIAEAIQGRVLDRGPVLG"
     gene            10687..11697
                     /gene="recA"
                     /locus_tag="PK710_05805"
     CDS             10687..11697
                     /gene="recA"
                     /locus_tag="PK710_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOT09263.1"
                     /translation="MLHLEEKLNSIRGLVSGIEKAFGKGAIMQLGDEQTTADIAVIRS
                     GSLALDEALGVGGYPRGRIVEIYGPEASGKTTLTLHAIAQAQANGGIAAFIDAEHAFD
                     VSYARTLGVDTEKLLVSQPDNGEQALEIVEMLTRSETVDLVVVDSVAALTPKAEIEGD
                     MGDQHMGLQARLMSQALRKLTAAAHRTGTTLMFINQLRQKIGVVFGSPETTTGGNALK
                     FYASVRLDVRRIGQLKVSDTVVGSKTRVKVVKNKLARPFQEAEFDIRWGTGIDSVSDL
                     LDMGMRSGVVTKAGSFFSFGSENLGQGREKSRETLLVREDLRLALSDAIRVAGLSNSL
                     AA"
     gene            11976..12992
                     /gene="hemB"
                     /locus_tag="PK710_05810"
     CDS             11976..12992
                     /gene="hemB"
                     /locus_tag="PK710_05810"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOT09264.1"
                     /translation="MPDAYGVDTLADLMMDLISRPRRNRKSEAIRAFHRETVLAPQHL
                     VYPVFIHDGKGTQPIASMPGCARLSLEALLAEAEGALSEGVSAMVLFPAIADELKSSQ
                     GEEAYRADGLVPRAIRALKNRFPELIVVTDVALDPYSSDGHDGIVQGGKILNDETVEV
                     LCKQALCQAQAGADIIAPSDMMDGRVAAIRDTLDDAGFTDVSILSYTAKYASAFYGPF
                     RDALDSAPKFGDKKTYQMDPANAREALRELALDESEGADMVMVKPAGPYLDVIHRVRE
                     YTTLPVAAYQVSGEYAMLKAASQNGWLEEKRVVLESLLGIRRAGADVILTYYARQAAR
                     WLRQ"
     gene            13191..>14195
                     /locus_tag="PK710_05815"
     CDS             13191..>14195
                     /locus_tag="PK710_05815"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT09265.1"
                     /translation="MLPEEAQLEALRDQVVGGFLTGSRGVRYYLRDLLGEGGQGWVYK
                     ANYDEPDGMWVVVKLLRPDSVNPEALRRFQREAEVLRMMGMQPTPCPHVVRFYDHGIT
                     RFHVGEGASARVLDLPFTVLEYVDGITLADVIDGSPGKGLSVGRVRRILRQVVRALET
                     VHAHRIVHRDLKPSNILLMSQSRQEVVKVTDFGLVKLVDLKMNQTTAFAGATLGYAPP
                     EQYEHGNERVSEQSDVFSLAAILFECLAGRAAFPYADGDPPLRVITQIIMGARPSLAD
                     CANNLSLELQGQDDLIQRLDRAITQGLQPDPSNRQPTCRALCERHGAQRAGPAGQPEG
                     E"
BASE COUNT         2850 a         4102 c         4322 g         2921 t
ORIGIN      
        1 tctcccacga cctctagttg acgcgccaag gtcacccggt tttcgccgga aacgtgaagc
       61 atgatcaact cgagcagcga tcgtttcgcc gtccaatcgc gcacttcaca ctgcagtttt
      121 gcctctaaaa acacatcata ggcgctggtt ccgcccagcc tctgttgcca ggcaccgcgt
      181 cgcgcttgca acggaagctg tgatgtcgtc gaacacttcg tgcgtcccgt gggtttcgcc
      241 ggcggaagct gaatcgtatg ggttactttt atcttggtgg tcagcccact ggctgggaaa
      301 gaaagccctg aaatcacgga agccacacag gaaaatagcg cgggatcgta agcagagcgg
      361 cttccctcca catgcacatc cgaagcgcca ccgttgccat ccacttgaaa agagacatcg
      421 agcacgcccg taaggtccgg tcgcagcgca gcgcgcgaat ccctgcaagc acgaatgccc
      481 ccaatagcgg catccaacgt gcgtttggcg gccatccgca ccgccatttc gaaacccgct
      541 tgccccatgc ccacgttgcc catcggttcc gaggaaagat cgagcatcgt gggggcaagt
      601 tcctggggag tagcaaattc cgcggaaaaa atagcgtctt gttccatggg cagatctagc
      661 aagcgctcca ccatggaaag ctcgggatac ctaggccttt ctcccgtgtt caacgtccag
      721 cccgtccacg gcgtcatgag tttttcccgt aacgccacgt ccgtgacggc ttcccgcccc
      781 cgagaccgca aggcaagctc ctcgacgcgc gccaaagccc atcgttttcg aacgtcagta
      841 ccgtctgcga tacgctcccg tttgaccaag agtttcttcg tttcttcacc gttttcaccg
      901 cgccaccgga gcgtgacctc acgaggcgct tgctcgcgcg tgcgtcccac ggcaaatacg
      961 gtcgttccag ccaccaccgc ctgcgcgttc cacggatata cctgctcgac atgaggccca
     1021 aggtcgatcg ccacggaggc aactgtcggt tgcagcgcat cggcgagaag ttgatgagcg
     1081 gcttcgggtg cttcgttcgc gttttcgacg tgcaagaccg ggccgaggcc acgcaccagc
     1141 gccatgagac caaagcgatt cgagcgcgaa cccacggata tcgcgccgag ccgtggggat
     1201 cctccggttc gacgcgccag acgcgctctg atttcccttg cttgcagatc ccccacggaa
     1261 ggccagccat ctcctacata gacgaccagg cccgcggggt ctttgggatc gagacgatcc
     1321 gcggcggctt cgatggccag tccgaggtcg gaagcaccag cgggttcgag acccccgagc
     1381 gctgtgatca ccgctttttg acgcgcttca tccatggcgc cgatcgcttc cggccccacg
     1441 gattgcgcgg caatgttggc gcccaacacc accgcctggt cgttgggacc cagggaacga
     1501 accaaggttt ccacgaaatt tcttgccgca tcgaaagctc ccgcatccat gctcgaagaa
     1561 gtatccagga cgatcgcaag ccgagcaccc cgctttcgct cgggctccgg tgcctccgcc
     1621 cgaacgagga cgtaagagcc tgcttcatca tcgtcgtcgc cctccgccat ataaagccgt
     1681 gccgggcttt tatcaggcgc catctccagc tccaccacga agtccgccga agggcggaag
     1741 tccgacttgc gcagcatcac aacgccgttt tcgacggtcg ccccatggcc acagcgaatc
     1801 gaacggggag gcgtcaccgt ggtatccacc tgcaccgaga attcaccaat caacggaggc
     1861 tccgcatcgg aagcgagcgg gaaccgatac tccaccacca cgccatcgtt ggttttgcga
     1921 cgcggcaacc attgcgcata gcgcaccacc acatgcacca cggacccggg ggaaataccc
     1981 cgcaacgtgc cacgaaccca gccatcgccc gcccattcga gcgtcggttc cgaagtcgtt
     2041 gccgagaaat tcgtgtcttt caacccgata cggccttcac gaagagaatc acccactccc
     2101 gcggcaaatc cggatacgat cgctcccgcg ggtagcgcca tgcgaaaatc tccccacaca
     2161 ggctcgcttc ccgcatggaa aaacgtcgaa tgcacgaccg tggacgccgt ttccccaatc
     2221 acagtagccg caacgttttg tgatcgaagg gtcaagggtg atcctggatc gccgactgtg
     2281 gcccccgagc tgccccataa ggttcccacc gcccgacgca tcgacccggt cgcgctccaa
     2341 ggcgctacca gtcctcccgt ccaatcgtca aaaacgcggg ctggcgcgac tttgaccacg
     2401 gtcccttcga tggaagccgt ttcccccgtt tgcacggtgt ggtccttgcc cgccgcctgc
     2461 accacaagct ctccggaagc agcgtagacg gacgcttttt tcgtgtcctg ggcatcgcgc
     2521 tcgatacccg cgtgaccgcc tgtcatgatc gccgaagcct cccccaattc gatggtcgtc
     2581 cgggctccga tggcacctac gagaaacaga cgcccccgct cgagtcgaag cgttcccgcg
     2641 gtcagcgtca ccttggtatt tccatccacg atcgccaccg tgccatcgtc aagacgcagg
     2701 cgggctcgcc cctcaggctt ggtttcgatg acccccgcat cccccacgcg gtgggtttga
     2761 agccgatcac tgccgttcag cgagacgcct tcgtgaacga cctgcaggtc cgcgacggag
     2821 gcttcgatgg ttccgtcttt tttgcaagag gcaaatacga caagcagggt gagccacaac
     2881 cacctggtaa cgtgaaaaat tcgggtactc atggcttcac ctccattccg tcgacgcgga
     2941 agcggagtgc ggcaggacct tcggggttga agacgacggg cacccgaacg actttttcgt
     3001 cggctttgcc ctcttggaag ataacggtga gaagagcctg ggccccgagg cgggcgatgt
     3061 ttgtaagatc gcgtttttca atacgaattt ctagtaccat tccatctctt cgcggcagga
     3121 tgacctggga aaggcccaag gtcatgtcgc cttcgggcgc tggcatcatc gagccgagag
     3181 cgttgctcca cagcgtggga tgcagttcgg gatgggacca taccagcacg gcgcgcgcgg
     3241 tgttttcgga acgatcgtct tgggaaagca gccgcatggt gcgttgtcga agcaattcga
     3301 attcgtcatt ccggttttcc gctgcggctt gcactcgtcc ccaggcaagg aaaaccgcgg
     3361 caagagagcg cgcggtgcgg gccagccctt gtgcatcacc ccccggagcc ccgtcgttac
     3421 tcgctttttc agcccatcgt acggcttcgt cgagcttgcc aagcccctgg gccgccgagg
     3481 cttggagcaa aaagaccccg gtttcgtccg gcgcgagccg ggccagggtt tcgtattgcc
     3541 gcgccgcttc cgcaaaccaa ccatggctcc gaagcagatc ccccagcctg cgtcttgcca
     3601 caggctcgtc gggtgaatat tcgacgattt cgccgaaagc tcttcgtgct tccgcctcgt
     3661 cttccgatgc cagctctttc gacgctgatc gcttggcaag ccgcaagtaa agttcgccca
     3721 cttcggtgag cacgtgggca tctgcatcag ggcggttgcg cagttggcgg cccaaaacac
     3781 gagccgcgct atcgtcgttg gcatcttcca gggcgtgaag aagtgcgagc gaaagggttg
     3841 aatcatctgg ccattgaagg cgaaggtctc gaagcatgac ggcccgagcc gccggattgg
     3901 gctctttttt catcaattcc tcgagcaatc cagggtcgac ggagcgcagg ccaagcgctt
     3961 gatgcaattg tcgcatctcg tcggttttgc ggatgcgtga caagatgccg cggtaaagtg
     4021 aatccgccac gcttcgttgg gcttgcatga gcctccagag cgcaactcgt ttttccacgg
     4081 taggcaaggc atcgagcatc atggacagaa gccggcttcg ttccggccac gcggatgctt
     4141 cacactgatt gatggcttgg tggtagacgg aagcgacggc ctgcggctcg ccccttgcgt
     4201 ttccgagccg ttcacgccat agggccacac gatccgccag ggggagcttg gacccttgcg
     4261 cgcaaggaac tacctggtgg gggaggttga ggttgacaat aaactgatgg atattcaggg
     4321 gttgcgatgg ttttaccaaa ccttcggggg gagagaaagt gatggtatac gtcgcggcaa
     4381 ccggtcccga agaggcagga aacgacagtc gctcacacgc tcgcaatacg caggaaacga
     4441 caccagcatc gggaaatccc gtatccgcat cccttatgtt ggatagggag ccgtcggcgc
     4501 caatgtcgaa acgtaggttg atgcgtccac ggaggttggg attggattgg agccccgttt
     4561 cgtaacacat gcgaaaggcc ccgtagtttt ggcggaagat tcgttcgatg acgggcctgg
     4621 gaagcggacc ggtggcgtca gcgacatcca ttttcacctt gccggattgg ctctggtgcg
     4681 aacctcccag ccgcccatgt ccggaaccaa acccttgacc gatgccttct cctttgcctc
     4741 cgccgcctgc tccgatattc ataatgccga gcatcgaatc agccgttttg tcgtcgttgt
     4801 cggaacctgt ttgtatggca gatccggcta ccgcctcggc ggtggtggtg gacaggggtg
     4861 ctggttgggc ttggggtggc ggtgacgggg cacctccacg gtccttctcg cttagcgcac
     4921 tgaccttcgc cgtctcgccg cttgagtcag tgggccctct agctgcgaat gtcgagcttt
     4981 tgcgtcgccc actgccctcc tcggccttct tttcggcata atcctgctgt tgctcgagtt
     5041 ttacggtctc cgggatgtcg ccgggcgcca cctcggcgag cggcgccgct tccatggtgg
     5101 ccgtcttttc cgatttcgac tggcatccgg caagcagcaa cgagttaagc caggcatact
     5161 tgctttccgg ttcggtggga agcagggcgc cacgggggtt tttgtcgaga tttcgctgat
     5221 tttttcgaag ctgctccaac tcatccgacg acatttcctg agttgttggc acatagagcg
     5281 atgtgtatgg tgtgatgatc ccaccgcgaa cgccgagatc gacgagtgcg gctcttccca
     5341 caccgtccgc gagcaattgc cgcaaccggc ccgtggacca gcgagcttgt aagtcaccaa
     5401 aatccgcgag acgttgaggg ctaatggcga gggtgttatc ccccgcgggc gtggtaattc
     5461 ggatggtttt ggggggatca ccggtgagtc ggcccacgac gatgacgctt tcgtcggcga
     5521 tgagggtttt gagttcgcgg ggtacgagcc gttcgacagc gggccccagg tccacgttca
     5581 cgccgagcca ggcggggcgt tccgcttctt cgagcaatcg caaggcggtt cgagccgcgt
     5641 cggcggcgtc atccaaccgt tccgcgaacc cggcctccac cagcccttcg ataatagaca
     5701 tatccgcatc ttgcccaacg cccagcccga aagtgcggac agggcgcgga agtttggcaa
     5761 gccgctcttt caagtccacc agagccagtt cccccacggt gggtttacca tcaccgatgt
     5821 agatcagaac cccccgacgc gcgggatcca agcttgctgc ggcttgcgca atcattgccc
     5881 ccaaatccgt ggcgcccccc cgctccagcg tggcaagccc gatcgaagcg tctttgcgaa
     5941 ccgcttcggt caccggacga agcttatccc agccgcgaac cacaggacgt aacgtggtat
     6001 ccccggcaac gagcagggcg cgatcttgct cgcccaggtg tgacaaaagc gcatgaaccg
     6061 ccgctcgggt caccaccatc gaggcgttat ccatacccgc ggagctatcc accaccatga
     6121 cgaggtcgag tcccccttcc tgttttggaa tatccgaggc gcgaatgggg atcgccagat
     6181 aagaagcctc ctcgatcgct cgttttctcg cttccgcccg atccgtgggc gcaaccaatt
     6241 cgaaatccac cgtatgcggg gcaaggtagg cttgtgaacc cttcgcccca tcgtcgaata
     6301 attccaccgc aagatccgcg cggggcacca aatcgtgagc gcgaatcaca accttatcct
     6361 tctcgatcac accttgcatt cccgtgcgaa cctctttcgc ccccgctcga cccaagtcga
     6421 aggtcgctcc gaaaaattcg atatgaggaa gagtctcttc cgcgccttcc gcggccatcg
     6481 gaaacgtgta caaacgtcgc tcaccacggc ttcccgtgcg tccaagccat tgcgtataac
     6541 gaaccacgac acgacgggta gcgccggcag caatcgggta aagacgagcg cgatagactc
     6601 caggggagtc ccactcgagc aaagccgggt cttccttgga accttcataa acatgggaag
     6661 cgtattgagc cgcggccgcc attttttcct tgacgcgacc ccagacgatg acaccttctc
     6721 gatctacgcc aaagcgagtc aacgatgcgc cgatgggaac acgaaacgtg taaacgcctt
     6781 ccacggtttg cgatgccccg ttgaaaaaca tctgatcgac ttccgtgatc gcaagatctc
     6841 cttcgaccga tacgcgcact tccatgcgac gaatggcaag aggcgtacga ggctgccctt
     6901 gttctcccgg caaacgcgct cccaccgtcc ctaccccaaa aggagcaggc tccgcgcctc
     6961 gatccgtcgt ggccagacct cctgtccagt ctacccacgc gagcgccggt tgcaccaatg
     7021 gcttacgatc ccgagcgagt gacatacgct cgccgggccc cgcccggatt ccatccggcg
     7081 tccgcacttc tccggacaat acataagcct cggtttcctc gcctatctcg agactcgcgc
     7141 gcacacggga taggtataac gagcccaccg cagacgtgat ggtcgtgacc tgatcatcgg
     7201 gagagtcgat gaagacctga ccctttttta catggatatc atcgacacca aatacgaccg
     7261 tggccggacc tcgcaggagc acggtggaac cgccatcgcg acgaagccat gctagagcgt
     7321 tgggggcaac ttcgatggtg gaaccatcgg tgaggcgttc acgaggtagt atcgatcgag
     7381 aggaggcacc ggcgggtgct accgtgatgg cgcctcggat cgcccgaatt tcaccgaatg
     7441 tttgggattg gggagccaat gattgtttcg gtccgcacga accgatgatg agcaacagga
     7501 agacaacgag aaccgaatgc acgattcgag aacacgatgg catgagatgg accctccaat
     7561 ggctctagcg taccggaacg gtaagaccac ggacacaagc aaatcttggt tgttccttgg
     7621 gaagtcggtc gacaagggga aaatgttgta aaaacaatgg tataggggct tttggggtcg
     7681 cgacggcggc ggcgctctcg agatgattcg ccgtcaaatc gaaaggatgt tcagggggat
     7741 tgcggaagca cttttctgcc gttgaacatg gggccgaggt cgagggccgt gaagcctgct
     7801 tttcgcaagg ttttcgcggc ataggcgctt cgcgcgccgg aagcgcaata aaccacgacc
     7861 ggtctggttt tgtccttcag ccgttcggga tgaagatcgt tgacggggat atggatggcc
     7921 cctggaagat gacccgaggc aaactcggct tcggagcgaa catcgatgag cgtggcaccc
     7981 gagcgcaagg cttccgcaat ggcttggagt tgtttgggag aacgataagc tcccgccatt
     8041 ttccacagca aaagcagggc gatcacgacg aaaaggggaa cgatccaggg ctccatggct
     8101 gccttcttgg gcaggcaacg ggtcgtggca aggtggggcg gtcgaatgtt tttatgcagg
     8161 ggggcagggc aggaaggaag tgagcaactt aatgctacta tgttgaaatg attccctttt
     8221 ttcttggctc agcggcccgg gacgatggat ggctaccctt gtttcggacc aggccagaga
     8281 gataaggcgc gtatcgagca aagcgcgccg gaggccgtcg tgaagctgct ggtgggggtt
     8341 ggggctggac gacgttacgc ggaatcgttg gtggaagagg ggatcttcgg gaaaagtgat
     8401 ttcgcgaaag cccatccaat tcgcgacacg atgccatttt cggcgcggtt ggacgaagcc
     8461 gcgatgggtc catgtcgggg caaggtttgc ttcgacgatg gcgtgggttt gggtgagcag
     8521 gggttgttgc aaaggtccgg cgatcggacg cgttccgagt tcgtaggaga ggaagggaag
     8581 gtctcccacg caagcctttt ggcagtggtc gatgaataga tgcaaaaccc catggtggaa
     8641 ccagggcaac gctcgtcgtt gcgcgatgaa atcccgcgtg gatcctggca cggaacaggc
     8701 ggtgaaaggg ctggtgaggg gcatcgtcat gagcgatatc ctgggacaaa aaggtgtacc
     8761 gcagcatggt ggaacaggaa atgaggcatg tcgtcgtcca tgggaagggt accgcgttgt
     8821 ggggccaagg cgcaacgtca tgttgagggc aagggatggg gtggcggtgg ccagagtggg
     8881 ggtggcgaat ggtcgaaaaa cgataattca tacataaatc caataggtta caaagtggct
     8941 accccatcgg tttttccgga ttggggtggc gaaccgccac caggcttttg ggggaaacta
     9001 cggtaaacac cgcgaatttt cgtggggttc cgatggcacg acgtttgata gagcggggga
     9061 tatgcttgct actgcccata ccacggtatt gttgggaatg aacgcgcagc cggtccgcgt
     9121 agaagttgag tcttttcgcg gggttccgaa gttcgaattg gtcggcttgc ccgaggccag
     9181 cgtccgcgaa agctacgtgc gggtgcgctc cgccctcaat caattggggc tcaacatcgg
     9241 gcagcaacga ttggttgtca acttggctcc cgccgacctg cggaaaccgg ggagtgcttt
     9301 tgatttggcg attgcttctg ccgcgttggc ggcgctgggg catgtgaatc gagaatcatt
     9361 gcatgatacg ttgcttcttg gggaactgtc ccttcatggg gcggttcgcc cggtacgagg
     9421 cgtgctgcct caactgatgg ccgctcgaaa caacgggatg cgccacgcga tcgttcctgc
     9481 gggcaatggt cgggaagcga gcgccgtgga cggcatcgat gtcaaggtgg ccgatacgct
     9541 gcacgatgtg cgggagcatt tgcgcggaca gagcccgctg ccatcaccgc aggccgtggt
     9601 tttcgctcct tcatttgatg aacggcttga cctcatcgat gtcaaggggc aaccgggagc
     9661 gcgtcgagcg ctggaaatcg cggccgcggg aggtcatcat ttgttgatga ttgggcctcc
     9721 tgggggaggc aagaccatgt tggcgaggcg acttccctcg atcatgccgc cgctgaccta
     9781 ttccgaagcg ctcgaggtga ccgcgattca ttcggtggcg gggctgttgc cctcggattc
     9841 agggttggtg acgattcgac cttttcgagc gcctcatcat acggtgagcg acgccggatt
     9901 ggtgggtgga ggtgcgccac cgcgtcccgg agaggtttcc cttgcccacc agggggtgtt
     9961 gtttctcgat gaattggcgg agtttcgccg cagcgccatc gaggcccttc gccaacccct
    10021 ggaagatggc cagttgacca tcgcaagagc caaggaaagg gcaacctatc ccgcgcgccc
    10081 gatgctcgtg gccgcggtca acccttgccc gtgcgggtac gcgggggatt ccagtggccg
    10141 gtgtcagtgt ggagagcatc ggattcacgc gtatcgcgca aaactctcgg gccctttgct
    10201 ggatcggatt gacctgcacg tgcatgtgcc gccggtcgat gtgactttcc ttcgcgaaag
    10261 gccctgcgga gaaagctcgg cgaccgtacg tgctcgggtc atggccgcaa gagcgatcca
    10321 ggcacggcgg ctcgagacgg ggcaggtgga aagccgctgt aacggcgctc tatcctcgcg
    10381 agaactggat gccgtggccc tgcccgatgc gcaagggatg aaactgctga tcgctgccgc
    10441 cgagcggctt ggcctgtcgg cccgggccta cggaaaaatc cttcgtttgg cccgaacgat
    10501 cgcggacttg gaatcgagtc ccgatgtgcg ctccgttcat attgccgagg cgattcaagg
    10561 gcgggttctc gaccgagggc ccgtgctggg ttgaccgtcc gggcgttggg gctcggcgga
    10621 aatcacatga gcaggggaca ggtatgcgtt tgcgcaacgc cgcgttccct acaaggagaa
    10681 aaagtcatgt tgcatctaga agaaaagctc aacagtattc gtgggttggt gagtggcatc
    10741 gaaaaagcct ttggaaaagg ggcgatcatg cagcttgggg atgagcagac gaccgcggac
    10801 atcgcggtga ttcgaagtgg atcccttgcc ttggacgagg cgctgggtgt gggtggatat
    10861 ccgcgaggac gcatcgtgga gatctacggt cccgaagcga gcggaaagac cacgttgacg
    10921 cttcacgcca ttgcccaggc gcaggcgaac ggcggaattg ccgccttcat cgacgcggaa
    10981 cacgctttcg acgtgagcta cgcgcgaacg cttggtgtgg acacggagaa gttgctggtc
    11041 agccagccgg ataatgggga gcaagccctt gaaatcgtgg agatgttgac gcgctcggag
    11101 acggtcgatc tggtcgtggt cgattcggtg gccgctctta cccccaaagc cgagatcgaa
    11161 ggggatatgg gagatcagca tatgggcctt caggcccgcc tgatgagcca agcccttcga
    11221 aaactgacgg ccgccgcgca tcgtacgggc actaccctga tgttcatcaa tcaattgcgg
    11281 caaaaaatcg gtgtcgtttt tggaagcccc gagacgacca ccggcggaaa tgccctgaaa
    11341 ttttacgcca gtgtgcgcct ggacgtgcga cgaatcggcc aactcaaagt aagtgatacc
    11401 gtcgtgggct caaaaacccg tgtcaaagtc gtaaaaaaca agctggcccg tccgttccag
    11461 gaagccgagt tcgatatccg atggggcacg ggcatcgata gcgtttccga cctgcttgat
    11521 atggggatgc gctcgggcgt cgtgaccaaa gccggttcct tcttttcttt cggttcggag
    11581 aaccttgggc agggtcgaga aaaaagtcgt gaaacgttgc tggtacggga agaccttcga
    11641 cttgccctct ccgatgccat ccgggtagcg ggtttatcga attctctcgc tgcctagaag
    11701 gataaatatt caataaattc aatatattac acgataacca caaggcgtgg acggcgcatt
    11761 tcggcccttg ggcccccccg aaatctcagc tttggtgtgc ggaatccggg tttgggggaa
    11821 gcgtcggggc cgatgggcag aagggtcggg gccgaggtgt cgtgaatcca tgataacctg
    11881 ttgaatttat gtaatatttg tgtgtccggg catgacgaga cgatatctgg agcggggctt
    11941 acgccgcgcg gtgcatcgat gccgagcatc tgctcttgcc cgatgcttac ggtgtcgata
    12001 cgctagccga cctcatgatg gatctcattt ctcgaccccg ccgcaaccgc aagtccgaag
    12061 ccattcgtgc ttttcaccgt gaaaccgtct tggctcccca acacctcgtc tatccggttt
    12121 tcatccatga tgggaagggg acgcaaccca ttgcatcgat gcctggctgc gcgcggctga
    12181 gtctcgaagc gctgcttgcc gaagcggaag gtgcgttatc cgagggtgtt agtgcgatgg
    12241 tattatttcc ggccattgcg gatgaactca agtccagcca aggggaggag gcctaccgtg
    12301 ccgatggtct ggttccacgg gctatccgtg cgctcaaaaa ccgtttcccc gagttgattg
    12361 tcgtcaccga tgtcgccttg gacccctatt cgtccgatgg gcacgacggc attgtgcagg
    12421 gaggcaagat cctcaacgat gagacggtgg aagtcttgtg caagcaggcg ctgtgtcagg
    12481 cgcaggccgg tgcggatatc attgcgccga gcgacatgat ggatggtcgg gtggcagcga
    12541 ttcgcgatac gctcgacgac gcgggattca ccgatgtttc gatcctgtcg tatacggcca
    12601 agtacgcatc ggctttttat ggtccgttcc gggatgcgtt ggattcggcc cccaaatttg
    12661 gtgacaaaaa aacgtaccag atggatcccg ccaatgctcg ggaggcgttg cgtgaactcg
    12721 ccctcgatga gagcgaaggc gccgatatgg tcatggtcaa acccgccgga ccttatctcg
    12781 atgtgatcca ccgagtgcgc gagtacacca ccttgcctgt cgccgcgtac caggtcagcg
    12841 gtgaatacgc catgctcaaa gccgccagcc aaaacgggtg gctcgaagaa aaacgcgtcg
    12901 ttctcgaaag cctgctagga attcgtcgcg ccggggccga tgtgatcctc acctattatg
    12961 cgcgtcaagc ggcaaggtgg ctgcgacagt agcgtcccca tcccctcgaa gcggtcgggt
    13021 tttcgacgcc tgtcgtggag aatcgttttc cccgtgcatg gccgggtttg ttgtaactat
    13081 ttgaatataa aatgaaattt catagtaggg ctctgtcttc tcgacagcgg ggctagattc
    13141 cgaacaagca tcttcgaacg tggcccttgg tgtattagca tgggcgggtc atgcttcccg
    13201 aggaagctca actcgaagct ctccgtgatc aagtcgtggg tggtttcctg acaggaagcc
    13261 gaggtgtccg gtattatttg cgtgatctgc tcggcgaggg ggggcagggc tgggtataca
    13321 aagcgaatta cgatgagccg gatggcatgt gggtcgtggt caagcttctt cgacccgata
    13381 gcgtcaatcc ggaggcgctg cgaaggtttc agcgggaagc agaagtgctg cgtatgatgg
    13441 ggatgcagcc cacgccctgt ccgcacgtgg ttcgattcta cgatcatggc atcacgcgtt
    13501 ttcatgttgg cgaaggggcg agcgcgcggg tgctggatct tccgtttacg gtgttggagt
    13561 atgtcgatgg catcacgctg gcggatgtga ttgacggttc gcctggcaag gggctttcgg
    13621 tggggcgagt gcggcggatc cttcgtcagg tggtacgggc tttggaaacg gttcacgccc
    13681 atcgcatcgt gcatcgggat ttgaagcctt cgaatattct gctcatgtct caatcgcgac
    13741 aggaggtggt caaggtaacg gactttgggt tggtcaagct ggtggacttg aagatgaacc
    13801 agaccacggc gtttgcggga gcgacgttgg gttatgcgcc tccggaacaa tacgaacacg
    13861 gcaatgaaag ggtttccgaa caatccgacg tttttagtct tgcggcgatt ttattcgagt
    13921 gtttggcggg gcgcgccgcg tttccctatg cggacggtga tccgcccctg cgtgtgatta
    13981 cgcaaatcat catgggggcg cgtccttccc tcgcggattg tgcgaataac ctctccctcg
    14041 aacttcaagg ccaggatgat ctcattcagc gtttggatcg agcgattacc caagggcttc
    14101 agcctgaccc ctcgaatcgg cagccaacct gccgcgcgtt gtgcgagagg catggtgcgc
    14161 agcgggctgg ccccgctggc caaccggaag gcgaa
//
LOCUS       DAOSOR010000072        14149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4322_length_14149_cov_3.955797, whole genome shotgun sequence.
ACCESSION   DAOSOR010000072 DAOSOR010000000
VERSION     DAOSOR010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 14149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14149
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4322_length_14149_cov_3.955797"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1282)
                     /locus_tag="PK710_05820"
     CDS             complement(104..1282)
                     /locus_tag="PK710_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT09266.1"
                     /translation="MSTISIVDSSLDSVSISATAPRNHSPYAIRVRHLWHRFGAFDVL
                     RDVTFDVEHGQIFGFIGPNGAGKTTTIRIIATLLEPMAGRVEVDGYDVAVDSESVRAI
                     IGYMPDHAGVYERVTVREYLEFFADAYQIPHLGVVETVMELTDLEKLQDKLVVEMSKG
                     MKQRLQLARVLLHDPKILVLDEPASDLDPRARIEMRDLLLELRALGKTIFLSSHILTE
                     LADVCTSVAILERGALVVAGPIAGIAEQLERRRYAMAYGHHAAEDRPAQLPSSPQGQP
                     QQASATGVEAPGGQSAVQPAVTPGGVPVPGSARKRLKLRVLTDANQAAQLLWGGPGIL
                     EVEVHASGNLLVGYQGSDALIADLVRHLVTHGVAVVGVEPERSELERIFLEVTQGEVQ
                     "
     gene            complement(1279..3345)
                     /locus_tag="PK710_05825"
     CDS             complement(1279..3345)
                     /locus_tag="PK710_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09267.1"
                     /translation="MQRAWVSAFVGIVLSLLSMTTARAELRVSAAPVMGANTPINGAW
                     IECVVRIENDGPRLEQGEVEIRSAAGYGVNDELRVTTTFAVSSGAAVNVRLPTRVFVN
                     AGGSLLLVVTNEAGEEVATTTLPTLGSAVPFLLDATEPPRLAGALRDAPTLLSFDPGN
                     LPPRTHASGRVNVATGGVRLDTSTGDPVLPDRAAGYAAATLVVMRTDQLSRLTGMQLD
                     ALANYVLSGGTLALAVARPEDLRSEPLISMTGGEIKTVPVSRALRRMPAEKIPDPDDR
                     GSGGGDPDLPGKLVTPGSEVDKDLVGYSGGNLRPTLFGAGASYGLGEVHVLAFDPTQA
                     PGVDDAWVKSRMLQLLTHAWERRRHLIVPHGTAFPDEYQVGGVFKLLDPNQSSRWAII
                     VAALLLLVYSVLAGPINFTRAARQQRPLRALWILPVMSSVTFFLMVFLGIFAKGWSGK
                     ARHLTLIEAASGMSKASACRYRAFFTSNSRKLTIRASDLSSVLDTVTVPRAGAKRALR
                     VDRDGVQLVGLSTIPWETLVIREDGFASLGAGVSITRDEQGGMVVTNRVARDLRAVIA
                     IEPATVRGGERNVYFFSRIRDGESKRMAEGVSMAFKPWRVRSSLTEVELEPIRHDLEK
                     ESKGLVAAWAAIGATVSNQVNWWPEDVPVVLAQMEGGEGYSSDAGLGVDMDRVLIRVV
                     GWGGVP"
     gene            complement(3351..4385)
                     /locus_tag="PK710_05830"
     CDS             complement(3351..4385)
                     /locus_tag="PK710_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT09268.1"
                     /translation="MTNLASFEVRLAQASQASQLLRGAIATVVVGQDAVVDQVLWGII
                     AGGHVLLEGAPGLGKTLLVRTLASALDLVFSRIQFTPDLMPSDVTGTTVLIMDAGGSG
                     GRHFSLHKGPIFGQVILADEINRATPKTQSALLEAMQEHAVTIAGERHRLQEPFFVLA
                     TENPIEMEGTYPLPEAQLDRFLLKVMVPMPTEDELTQILVRTTGHEVAPPAKVLGQDD
                     VLALRSLCRDIAVAEPVLRYAARMVAASDPSSSFAPEKVKQAVRFGAGVRGAQSLILS
                     SKAVAMLEGRAHVAFADVQRVALPCLRHRIIRSFEGEADGVTTDEIVQELIRSVPSRP
                     ESVDQAVRRG"
     gene            complement(4547..6394)
                     /locus_tag="PK710_05835"
     CDS             complement(4547..6394)
                     /locus_tag="PK710_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09269.1"
                     /translation="MRTEPRAEPNRFYGLFEKWARRVRARMTIHRALTGLAIGLVVGA
                     LLAVGAWRLRQGAVRPYMAAAGVVGLMLGAGWALRRRWSDVEVALYLDGRLASKETIT
                     TAIALRDEEGEDDAARAVVVTKAAEVLAAGNPKRARPAVLSAVHLLIPVGVGALVWVS
                     ITELPLAPVTAIEPGADIVRVAEIEALEKAVELGRLETPDPEQRERLKKIGEDARKLK
                     EQLEKGMERREAQSEMARLRDEIAAERARMSGSEQRAGLEAAHGRLAQEKMLKEAAKA
                     LGDRDLTRFDEEMQKLANQLEKQDRELAKKALEEAAEEARKNNAKDVAKMLEEQKRLL
                     EERAKRDEALRQFADAFGDDMPPQMKEQLDEFREQGTDESAGRLAERMADALKNMTPE
                     ERKRLAERLKEQMNKQDGESPGESMTKEQLEELADRLETPEGQRALEEMLRKMANEPP
                     HSDEAERDRALGDAEKGLGEAERELGQQGPQGPGQGQKGLGTAPVPVPNSGGTPGASP
                     GGNSTDKGNSKDMGGTGSHHDKGRGDHKGSTPEIEGDGFRARAGGKLNPGAPMPGVVT
                     GRGKGRAGESANVRGLDGIGEASTGEVGGVERSEIPEEYREQVGRYFSP"
     gene            6594..7067
                     /locus_tag="PK710_05840"
     CDS             6594..7067
                     /locus_tag="PK710_05840"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOT09270.1"
                     /translation="MGPSPFPREEYVENLSIEQALRNAVEAELAAARFYSRLAEHTTD
                     PTARRLVQQLHDDELKHAAAIELMSRRLGKVLPVHATDGYAMVEIEHEWDLDSNINYL
                     SVLERAIAAERKASRFYGTLATAFTGEARSFFDELSMVEELHANRLLKLLEKARG"
     gene            7163..>7785
                     /locus_tag="PK710_05845"
     CDS             7163..>7785
                     /locus_tag="PK710_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09271.1"
                     /translation="MRRCSVALLSLAGFLVMAHALGQEPESDAVTQMARQRFEEGVKL
                     FDRGDYKGAQAAFLQAWTLKQHPAVLLNLAQSELRSGDHVAAARHFATYLRDYPDTPA
                     KERAAAQQGLTEARKHTGQLEIMADDTNVEVLINNESVGRTPLPGPVDIAPGSYQIVG
                     RLAGSEPFESSGSVQAGQSKSVEITFHQLAPPPPPPPPPPPPPPPPPP"
     assembly_gap    7786..7795
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7796..8312
                     /locus_tag="PK710_05850"
     CDS             <7796..8312
                     /locus_tag="PK710_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09272.1"
                     /translation="VRPPPPSESSDRQPFLTWAQTDPIAWTTLGAAGLGIGIGTIFAV
                     LAKSKANDANSIASQISAVAQSDPGMSNYEGYNRRSDPCAPPVPVTPDTDFRPACSQL
                     QDELDTLDSRKTMMWVGFGVGAVGVVGTGVAYYLRSDKTNANTAALIDNTVVSPVWSN
                     HLGGIRITGTF"
     gene            8480..8656
                     /locus_tag="PK710_05855"
     CDS             8480..8656
                     /locus_tag="PK710_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09273.1"
                     /translation="MMIKINKFLRDNAGANLVEYIILVGVIALIAIAGFKIFGEKVDK
                     KIQDQAGKVEQIGN"
     gene            complement(8731..9525)
                     /locus_tag="PK710_05860"
     CDS             complement(8731..9525)
                     /locus_tag="PK710_05860"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HOT09274.1"
                     /translation="MFTLIIRERSGEERRELFEEQEVLVGRVPGNDLVLRAGNVSRQH
                     ARFAYRNTRLLVSDLGSANGTYVNGRKIRQPTILRDGDEVWIGDFYLRVEISSPASMQ
                     AVAAPAPAHPVVMTQWYVYREGGDTVGPVGTDQLCAGIRAGKVPLDSYVCAVGSHQWQ
                     PMDHVPDLAGAVRTVAEQAAQARQPAPATEMPSQLATDVGPEALWYVVSEGSDPFGPI
                     TTEQLRRAIRSGQVSITSQVNRAGSPDWFDLAVMPDFYEEVLRFGR"
     gene            complement(9734..11542)
                     /locus_tag="PK710_05865"
     CDS             complement(9734..11542)
                     /locus_tag="PK710_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09275.1"
                     /translation="MRALWLWTGLLFLLMPACAWFGNPEVPSAQPPEFVPPGGQAKCK
                     VTGSMMRPLIVEWPAADRASLEVLAQRGTVVVRYSGCEMEVLPRCKAPGVYRYVPTTR
                     KSERVVIQDEDELYANLPIGAAKLEGKLRRAGQLDVNMNVVGQYDAEQREVSQGMLEG
                     DCRKATHYITGLTVGAFEFSAGGTSEAGAGASGLGVGVGGETARRREILNSDGDVESC
                     EKSSLEDDKPPAGCGGLLRLELAELSTAPQPIVGPAVAEPVGEPTGDEPSCPEGMVRK
                     DGQCVALAGIGVGILGTAILPQTSRLHVQVYCGGDFGNQKVGQSDGLKVWVDDVLVPA
                     LEEVRVPNPMLPGDPGILLHVVYEVEPGDHKVRVGSDGCEDAERLVKVNPGSIRLVQG
                     RLRSTAWYARPPAASAGFGVGLNYMWFKPEFISVETASYEHESMDARTMSGLGLEIPF
                     STQYIWMAMGFGWTGGNFSGTAPECKYDVGNCLPKGTEVTGKIKGYHVPIWAAGRLPF
                     VYGAALLGSGIEFNILSMDFNEGENNFGTNTAQLNMHVPIWAGLEFRPTCGLSVSGRA
                     FRGFGLSGNMPPYNAAMVSLDLFATANCGDAGFGIQ"
     gene            complement(11592..12335)
                     /locus_tag="PK710_05870"
     CDS             complement(11592..12335)
                     /locus_tag="PK710_05870"
                     /inference="COORDINATES: protein motif:HMM:NF016932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HOT09276.1"
                     /translation="MAGKRHAREVDRGLPPKQSAQSVAPTLATVVEIEAHVARCIGEP
                     AFVWHELVSSLVHVDVHVVLPTENRPYFTLFTTGMSDRPMHVPDDVKHGKRLRYAELM
                     LMLPSGWFDDPRSLEELRDERKYWPIRLLKTLARFPHACDTWLAFGHSVPNGNPPAPF
                     VEGTKMCFALLVPPVRVPPEFYDLQLSDGRVVNIYGVVPLYPEELQLKVMRGTAALLE
                     RFDTQGVTELLDPFRDNTCGGEFDWRVVN"
     gene            complement(12465..13439)
                     /locus_tag="PK710_05875"
     CDS             complement(12465..13439)
                     /locus_tag="PK710_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09277.1"
                     /translation="MNGEKTYEMLWDCSFCGARKLLGLTHRHCPNCGAPQDPAKRYFP
                     PEEEKIAVQDHQYVGQDVLCPACQTANGRLAKHCGHCGSPLEGGKAAALQQEQVIPDH
                     VHSPSPAQAPKAKTGAKVGIGCAIGGIFGLIVLAIIGFFVLNSILKKDAGFKVVGHTW
                     THAVEIETYEYKKESSPCSKMPTSAERVTRQKQEPVCETRKIDQGDGTYKEKRECKDQ
                     EDICTYQVGRWVPKRSEKNTGSSVTDPMPWPTVTLSRPGECEGCEREGKKTATYTLRL
                     QDASSGKEKTCSFSDPEKWKSFQVGSSWKAKVGGISGGIDCDSLTPGP"
     gene            complement(13496..>14149)
                     /locus_tag="PK710_05880"
     CDS             complement(13496..>14149)
                     /locus_tag="PK710_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09278.1"
                     /translation="GPAVGLTQALLNEQLAMLAERLEAVNVSVFDDVSPVVWGSSDPF
                     QRGERGMDDALRIEKVWEACSRVGVDLLALLVKERAEVEQTLVDSRLPKSLSHATMRE
                     WTRLRAERPTLGVEEWRALLLSVRAWARIRQNSKLKLVRPRARIVVRQEGIGFLAKPF
                     AATYWIVAVFYGDFSELHAEGMVVRAIPRIERLVMALPPLVPPPARGRVLRLRRPKG"
BASE COUNT         3157 a         4980 c         3160 g         2842 t
ORIGIN      
        1 ggattaggat tgtttcgaac acgccgaagg ctcgttcgcg ccaaatccac cgcccgcgac
       61 gaaggtggcg aaggaacgaa cgaaggagtc ccggtctggg cactcattgc acttctccct
      121 gggtgacttc gaggaagatc cgctccaatt cgcttcgctc aggctcgaca cccacaacgg
      181 ctaccccatg ggtcaccaga tggcgcacca ggtccgcaat caacgcgtcc gaaccttgat
      241 aaccgaccag caaattgccg ctcgcatgca cctcgacttc caaaataccc ggaccacccc
      301 aaagaagctg cgccgcttgg ttggcatccg tcaacacgcg aagcttaaga cgttttcgcg
      361 ccgaacctgg aaccggtact ccccccggcg ttaccgcggg ttgaaccgcc gactgccccc
      421 ctggcgcttc cacccccgtc gcactcgcct gctgtggttg cccctgcggc gacgacggaa
      481 gctgtgctgg ccgatcctcg gctgcgtgat gaccgtacgc catggcgtaa cgccgacgtt
      541 caagctgttc cgcaatcccc gcgatggggc cggcaaccac caatgcccct cgctccaaaa
      601 tcgccaccga tgtgcataca tccgcaagtt ccgtaaggat atgcgacgat agaaaaatcg
      661 tttttcccaa cgcgcgaagt tcgagcaaca agtcacgcat ctcgatgcgc gcacgaggat
      721 ccaagtcgct cgcgggctca tccaacacca ggattttagg gtcgtgcagc aatacccgcg
      781 caagctgcag acgctgcttc atccccttgg acatctcgac cacgagcttg tcctgaagct
      841 tctccagatc cgtcagttcc atcaccgtct cgacgacccc aagatgcgga atttgatagg
      901 cgtcggcgaa aaactccaga tactcgcgaa ccgtcactcg ctcatacacc cccgcatgat
      961 ccggcatgta cccgattatc gcccggaccg actcagaatc gacagccaca tcatagccgt
     1021 ccacctccac ccgccccgcc atcggctcga gcaacgtcgc gataatccgt atcgtcgttg
     1081 tctttcccgc cccgttcgga ccaatgaatc caaatatctg accatgctcc acatcgaagg
     1141 tcacgtctcg caacacatcg aaggccccga aacgatgcca caaatgacga acccgaatcg
     1201 catacggact gtgattgcga ggcgccgtcg cagaaatcga tactgaatca agggaagaat
     1261 ccacgatcga gatggtactc atggcactcc cccccaaccg accacgcgaa tcaacacacg
     1321 gtccatgtcc actcccaatc ccgcgtcact gctgtacccc tcccctccct ccatctgcgc
     1381 caaaacaacc ggcacatcct caggccacca attcacttga ttgctaaccg tcgcacctat
     1441 cgccgcccat gcggctacaa gccccttcga ttctttctcc aaatcgtgtc gaatcggctc
     1501 gagttccacc tccgtcaacg aacttctcac ccgccacggt ttgaacgcca tcgacacccc
     1561 ctcggccatc cgtttgctct ccccatcacg aatccgagag aaaaaataca cgttgcgctc
     1621 cccgcctcgc accgtcgccg gctcaatcgc tatcaccgcg cgcaagtccc gagccacccg
     1681 gttggtcacg accatccccc cttgctcatc acgcgtaatg ctcacccctg cccccagcga
     1741 cgcaaaccca tcttcccgaa tcaccagcgt ttcccacggt atggtcgaaa ggcccactag
     1801 ctgaaccccg tcccgatcga cacgcaacgc ccgcttcgcc cccgcccggg gtaccgtcac
     1861 ggtatcaaga acgctactca agtctgaggc acggatcgtc agttttctcg aattcgaagt
     1921 aaaaaacgct cgataccggc aggccgaggc cttggacatg cccgatgcag cctcaatcaa
     1981 cgtcaaatgc cttgcttttc ccgaccagcc tttcgcgaaa atgcctaaaa acaccatgag
     2041 aaaaaacgta acggacgaca tcacgggcaa gatccaaagc gcgcgcaaag gacgctgttg
     2101 acgcgcggcg cgcgtgaagt tgatgggccc cgccaatacc gaatacacga gcaacaacaa
     2161 ggccgccaca atgatggccc aacgactgct ctggttcgga tccaggagtt tgaacacccc
     2221 acccacctga tactcgtcgg gaaatgccgt gccgtggggc acgatgagat ggcgacggcg
     2281 ttcccacgcg tgcgtgagca attgaagcat ccgtgacttc acccaggcat cgtccacacc
     2341 cggcgcttgc gtgggatcga aagcaagaac gtgaacctca ccaagaccat agcttgcccc
     2401 cgcaccaaat aacgtcggcc gtagattgcc tcccgaataa ccaaccaagt ccttatccac
     2461 ttcactcccc ggtgtgacca acttgcccgg caggtccgga tccccccccc cggaaccccg
     2521 atcatcggga tcaggtattt tttccgcggg catgcgacga agcgcccgag acaccggtac
     2581 ggttttgatt tcacctcccg tcatggaaat caaaggctcg cttcgtagat cctcggggcg
     2641 tgccactgcc aacgccaacg tcccgcccga tagaacataa ttcgccaacg catcgagctg
     2701 catccccgtc aaacgcgaaa gctggtccgt tcgcatcacc accagcgtcg ccgccgcata
     2761 ccccgccgcc cgatccggca ataccggatc gcccgtcgac gtatccaaac gcactccccc
     2821 tgtcgccaca ttcactcttc cactggcatg cgttcgagga ggcaagttgc ccggatcgaa
     2881 agaaagcaac gtgggagcat cccgaagcgc tcccgcaagt ctcggcggct cggtggcatc
     2941 aagcagaaac ggcaccgccg aacccaatgt cggcaacgtc gtcgtagcca cctcctcgcc
     3001 agcctcgttg gtcaccacca acaacaacga acctccagca ttcacaaata cccgcgtcgg
     3061 aagccttacg ttgaccgctg cccccgacga caccgcaaag gtcgtcgtca cacgcaattc
     3121 gtcattgaca ccatatcccg ccgcactgcg aatctccacc tccccctgct ccaagcgcgg
     3181 gccatcattt tcgatgcgaa cgacacattc gatccatgcc ccattgatcg gtgtgttagc
     3241 ccccatcacc ggcgctgccg aaacgcgcaa ttctgcacgg gccgtcgtca tcgacaataa
     3301 tgacaacacg atccccacga aagcgcttac ccaggcgcgt tgcatcgccc ctaccctctc
     3361 cttaccgctt gatccaccga ttccggacga ctcggcaccg aacgtatcaa ctcttgcacg
     3421 atctcatcgg tcgtaacccc atcggcttcc ccctcgaacg accggatgat tcgatgccga
     3481 agacaaggca aagccactcg ctgaacatct gcaaaagcca catgagcccg cccttcgagc
     3541 atcgccactg ccttcgaaga caaaatcagc gattgcgcgc cacgaacccc cgcaccaaaa
     3601 cgtacggctt gtttgacttt ctccggcgca aaagacgacg aaggatcgct ggccgcgacc
     3661 atccgcgccg catatcgcaa aacaggctcc gctaccgcta tgtcgcgaca caaggaacgc
     3721 aaagccagca catcgtcttg ccccaacacc ttcgccggtg gcgcaacctc atgccccgtc
     3781 gtacgcacga gaatctgcgt caactcgtct tccgtgggca tcggcaccat caccttgagc
     3841 aaaaaacggt caagctgcgc ttccggaaga ggatacgtgc cctccatctc tatcgggttt
     3901 tccgtggcga gaacgaaaaa cggctcctgt aaccgatgac gctcccccgc aatcgtcacc
     3961 gcatgctctt gcatggcttc caacaacgca ctttgcgtct tgggcgtcgc acgattgatc
     4021 tcatcggcaa gaatcacctg accaaaaatc ggccccttgt gcagcgaaaa atgccgtcca
     4081 ccgcttcccc ccgcatccat gatcaatacc gtggtgcccg tgacatcgct cggcatcaag
     4141 tccggcgtaa actgaattcg gctaaacacc agatccaacg cactcgccaa cgtgcgcaca
     4201 agcaaggtct ttcctagccc cggcgcccct tcgagcaata cgtgcccccc cgcgatgatc
     4261 ccccataaga cttgatcaac caccgcatcc tgaccaacca ccaccgtcgc aatcgctccc
     4321 ctgagcagtt gactcgcttg cgatgcttgc gccaatcgga cttcgaacga ggctaagttc
     4381 gtcatgctgg ttcctggaca agaaccctag cgcgactcgc taagcccgca aagcattggc
     4441 ttccacgaac ctcacacgat tcaatcttcc ccccaccacg accgaacgcc attccatccc
     4501 ccggcgggag aaataaaaat ataataatta cagcaagtta cgcctatcaa ggactgaaat
     4561 agcgacccac ttgctctcgg tattcttccg ggatctcgga gcgctccact ccccctacct
     4621 caccggtgga tgcctcgcca atcccatcca aaccacgaac attcgcagac tcccccgcac
     4681 gcccctttcc cctgccggtc accaccccgg gcatcggcgc ccccggattc agcttccctc
     4741 cggcccgagc acgaaaccca tccccctcaa tctccggtgt gctgccctta tggtcccctc
     4801 gccctttgtc atgatgactc cccgtgcccc ccatatcctt ggaattaccc ttgtccgtag
     4861 agtttccgcc cggtgaagcc ccaggggtac cgccactgtt cggaacaggc acgggcgcgg
     4921 taccaagccc cttttgccct tgcccaggtc cctgcggccc ttgttgtcca agctcccgct
     4981 ccgcttcccc aagccccttc tccgcatccc ccaacgctct atcccgctcg gcctcgtcgc
     5041 tgtgcggcgg ctcgttcgcc atcttccgta acatctcctc gagcgcacgc tgcccctcgg
     5101 gcgtctcgag tctatccgca agttcctcca gttgttcttt cgtcatggac tcgcccggcg
     5161 attcaccgtc ttgcttgttc atctgctctt tcaaacgttc tgcaagacgt tttcgctctt
     5221 ccggtgtcat atttttcaaa gcatccgcca tccgctccgc caaccgtccg gcactttcat
     5281 ccgtcccttg ctcgcgaaat tcatcgagct gttctttcat ctgcggcggc atatcgtcgc
     5341 caaacgcatc ggcaaactga cgcagcgcct catcgcgctt ggctcgctcc tccaacaatc
     5401 gtttctgctc ctcgagcatc ttcgccacat ctttcgcgtt gttctttcgc gcttcttccg
     5461 cggcctcttc caatgctttt ttcgccagct cccgatcctg tttctcgagc tggttcgcaa
     5521 gcttctgcat ctcttcatcg aatcgcgtca ggtcccgatc gcccaatgcc ttcgccgctt
     5581 ccttgagcat cttttcctgc gccaatcggc cgtgcgccgc ctcaaggccc gcacgttgct
     5641 ccgagccgct catccgcgct cgctccgctg caatctcgtc ccgcaaacga gccatctcac
     5701 tttgcgcttc ccttcgctcc atgccctttt cgagttgttc cttgagctta cgcgcatcct
     5761 cgccaatttt tttcaatcgc tcgcgttgtt ccgggtccgg cgtctccaaa cgacctagct
     5821 ccaccgcctt ttccaaagcc tcgatctccg ccactcgcac gatatccgca ccgggctcga
     5881 tcgccgtcac aggcgccaac ggcagttccg tgatgctcac ccacaccaac gcccccaccc
     5941 ccacgggaat caaaaggtga acggcactca agaccgctgg cctcgcccgt ttcggattgc
     6001 ctgcggccaa tacttccgcc gctttcgtca ccaccaccgc ccgcgccgca tcgtcttccc
     6061 cctcctcgtc tcgcaacgcg atcgccgtcg ttatcgtctc tttcgaagcc aaccttccat
     6121 ccaagtacaa cgcaacttcc acatcgctcc atcggcgacg cagcgcccag ccggcaccga
     6181 gcatcaaccc caccacacct gccgcggcca tataaggccg cacggctccc tgccgcaatc
     6241 tccaggctcc tacggctagc aaagcaccaa cgaccaaacc aatggcgagt cccgtcaaag
     6301 cgcgatggat cgtcatgcgc gcccgcactc gacgcgccca tttctcgaac agtccgtaaa
     6361 accggtttgg ctctgctcgt ggttctgttc gcataagcgt atctgtagcg ctcctgcgca
     6421 cgtggagcaa tccaacacgc atggccttct tcgatgaaat tctctttcac cacgggattc
     6481 ccccacgaaa gacgaagaga aaacgatgaa gccccctttt tccccttgct tgcacgagcc
     6541 ccaaaacgct gggttcttcc tccttccatc gtggttcgtg atatccttgc tcaatcggtc
     6601 cttcaccatt cccgcgggag gagtacgtgg aaaacctcag catagagcaa gctcttcgca
     6661 atgccgtgga agcagagctt gccgctgcac ggttttattc acgcctggcg gaacatacga
     6721 ccgaccccac cgcgcgtcgg ctcgtgcaac aactgcacga cgatgaactc aaacacgctg
     6781 ccgccatcga actgatgtcc cggcgtttgg gcaaggtact tccggttcat gccactgacg
     6841 gctatgccat ggttgaaatc gagcacgaat gggatttaga tagtaatatc aattatttat
     6901 ccgtgctgga gcgagcgatt gccgcggaaa gaaaggcgtc acgtttttat gggaccctgg
     6961 cgacggcgtt cacgggcgag gcacggagtt tttttgatga gttatcgatg gtggaagaac
     7021 tgcatgccaa tcgattgctg aaacttctcg aaaaggcacg gggataaacg atgctcgaac
     7081 catatctttt gcttgttgga atgaaaaaac cacaacttct tcccttgccc tccggccagt
     7141 catcctctag gaaccaggca ttatgagacg atgctcggtt gcgctcctgt cgctcgcagg
     7201 gtttctggtg atggcccatg cgttagggca ggagccggaa tccgacgcag tcacccagat
     7261 ggctcgacaa cgcttcgaag aaggcgtcaa gctattcgat cgaggcgatt ataagggagc
     7321 gcaagctgca ttcctgcaag cctggaccct caaacaacac ccggccgtgt tgctcaacct
     7381 ggcgcaatcc gaacttcgaa gcggggacca cgttgccgca gcacgccatt tcgcaacgta
     7441 tctgcgcgac tatccagata caccagccaa agaacgagcc gcggcgcaac aaggcctcac
     7501 ggaagcccga aaacacaccg gccaactcga aatcatggcg gacgacacca atgtggaagt
     7561 cctcatcaac aatgaatccg tcggtcgaac cccgttgccc ggaccagtag atatcgcccc
     7621 cggctcatac caaatcgtcg gtcgcctcgc cggttccgaa ccgttcgaat cctccggctc
     7681 ggttcaagcc ggtcaaagca aatccgtcga gattactttc caccagcttg ctccaccccc
     7741 cccccccccc cctcctcccc cccccccccc cccccccccc cccccnnnnn nnnnnggttc
     7801 gtccccctcc cccatccgaa agctccgatc gtcagccctt cctcacctgg gcccagaccg
     7861 accccattgc ctggactacc ctcggcgccg ccggcctagg catcggaata ggaaccatct
     7921 ttgccgtcct agccaaatcc aaagccaacg atgccaactc catcgcgtcc caaatctcgg
     7981 ccgtagccca aagtgaccca ggcatgagta actacgaagg ctataaccga cgatcggatc
     8041 cgtgtgctcc ccccgttcct gtcacaccag ataccgattt tcgtccggca tgctcccaac
     8101 tgcaagacga actcgacacc ctggacagca gaaaaaccat gatgtgggtc gggttcggcg
     8161 tgggagccgt cggcgtcgta ggcacaggcg tggcctatta tctgcgctcg gataaaacca
     8221 atgccaatac cgccgcgctg atcgacaata ccgtcgtctc tcctgtctgg tcgaaccatc
     8281 tcggtggaat ccggatcaca ggtactttct gagacagagc aattgttgat cccactgatg
     8341 agaagtccta ttactcaccc caccttccgc ttgcctgtcc actgctcatt ttattgttgt
     8401 tttgattgat ttcatcttgg cacgaacgtt gtatatcact cgtatcaaag cggcctcaac
     8461 gccccaagga gacaacgcca tgatgatcaa gatcaacaag ttcctccgtg ataacgccgg
     8521 cgcaaacctc gtcgaataca tcatcctcgt tggcgtcatc gcgctcatcg ccattgccgg
     8581 attcaagatc tttggggaaa aggtcgataa gaagatccaa gaccaggccg gcaaggtcga
     8641 gcaaatcggg aactgatccg ctagctcttt gattatcagc ggagactacg gcgccgacaa
     8701 cgggcgtgtg ccgctctccc acacgccccc tcaacgccca aatcgaagca cttcctcata
     8761 aaaatctggc atgaccgcaa ggtcgaacca atctggcgaa ccagcgcgat tcacctgact
     8821 tgtaatcgat acctgcccac tgcgaatcgc tcggcgaagc tgctcggtcg taatcggacc
     8881 aaacggatcc gacccctcac tcaccacata ccaaagcgct tccggcccca catcggtggc
     8941 aagctgcgat ggcatctccg tggcaggcgc tggttgcctc gcttgcgccg cctgctccgc
     9001 caccgtccga accgcacccg ccaggtccgg tacatgatcc atcggctgcc attgatggct
     9061 gcctaccgca caaacataac tgtccaaagg cactttaccc gcgcgtatcc ccgcacaaag
     9121 ctgatccgtt cccaccggac ctaccgtatc ccccccctca cggtacacat accattgcgt
     9181 catcacaaca ggatgcgccg gcgctggtgc cgcaaccgct tgcatactcg ccggggatga
     9241 tatttccacc cgcaagtaga aatctcctat ccatacctca tccccatccc gcaggatcgt
     9301 cggttgccga atcttacgcc cattgacata agtgccgttc gcactgccca aatccgatac
     9361 caaaagccgc gtattccgat aggcaaaccg agcatgctgc cgggatacat ttcccgccct
     9421 gagcaccaaa tcgttgccag gcactcggcc caccaatacc tcctgctcct caaataactc
     9481 cctccgctcc tctccgcttc gctcccgaat gatcagcgtg aacatggagc acctcgcgaa
     9541 cgcaaacgaa tcccctccct ctcgtggctc acaccaccat ggtccgaaat ccaatcatcg
     9601 gagtattcag gggagttatg catgatcgtc ggaaaaaccc aaggcaaacc cgggctcctc
     9661 accgttacca tggtaggagg atttccttca agacgctaat tctacgccat cacacccctg
     9721 tcgccagcct ggctcattgg atcccaaacc ccgcgtctcc acaattcgcc gtcgcgaaca
     9781 gatccaaaga taccatcgcc gcattgtacg gtggcatatt tcccgacaga ccaaatcccc
     9841 gaaaggctcg ccctgatacc gacaacccac aggtaggccg aaattccagc cccgcccaaa
     9901 tcggcacatg catattcaac tgcgcggtat tagtaccaaa attattctct ccctcattga
     9961 aatccatact caagatgttg aattcgatcc ccgagcccaa taacgccgca ccgtacacga
    10021 acggtaaacg tcccgccgcc cagatcggaa catgataccc tttgattttt ccagtcactt
    10081 ccgtcccctt cggcaaacaa ttgcccacgt cgtacttaca ttccggagct gttcccgaaa
    10141 agttcccccc agtccaccca aatcccatcg ccatccagat gtattgggta gaaaacggta
    10201 tttccaaacc cagcccggac atcgtcctcg catccatgga ctcatgttca taagaagctg
    10261 tttccacact gataaactcg ggcttgaacc acatataatt caacccgacc ccgaaccccg
    10321 ccgaggctgc cggcggacgc gcataccaag ccgttgaacg tagtctgcct tgcacaagcc
    10381 gaatagatcc aggattgacc ttcaccagcc gctcggcgtc ctcacaccca tcactgccca
    10441 cacgcacctt atggtcacct ggctccactt catataccac gtggagcaat atccctggat
    10501 cccctggaag catcgggttg ggtacgcgta cctcctccaa agcgggcacg agtacatcat
    10561 ccacccatac tttcaaccca tcggattgcc caaccttttg atttccgaaa tcacctccgc
    10621 aatacacttg cacgtgcaac cgcgatgttt gcggcaaaat cgcagttccg agaatcccta
    10681 cccctatccc tgccaacgcc acacactgcc catcctttcg caccatgcct tcaggacagc
    10741 ttggttcatc gcccgttggc tccccgaccg gctccgccac cgctggtccc acaatcggct
    10801 gtggcgcggt agacaattcc gccaactcca atcgcaacaa ccctccacaa cccgccggcg
    10861 gtttatcatc ctccaacgac gacttttcgc agctttccac gtcgccgtcc gaattcaaaa
    10921 tctcccgacg acgcgccgtt tcacctccca cacccactcc aagccctgac gcccccgccc
    10981 cagcttccga cgttccccct gccgaaaact caaacgcccc cacggttagc cccgtgatat
    11041 aatgcgtcgc ttttcgacaa tccccctcga gcataccctg acttacctcc cgttgctccg
    11101 catcatattg tcctaccacg ttcatattga cgtcgagttg ccctgccctc ctcaacttgc
    11161 cctcgagttt ggccgcacct attggcaagt tcgcgtaaag ctcatcttca tcctgaatca
    11221 caaccctttc acttttgcgc gtggtcggca catagcggta aacaccaggc gctttgcacc
    11281 tgggaaggac ttccatttca cagccggaat agcgcacaac aaccgtcccc ctctgcgcca
    11341 acacttcgag agacgctcga tccgccgcgg gccattccac aatcaagggc cgcatcatgc
    11401 ttcccgttac cttgcacttc gcctgccctc cagggggaac gaactcaggc ggctgggccg
    11461 acgggacttc cggattccca aaccaggcgc atgcaggcat caataaaaaa agcaagccag
    11521 tccacaacca caaagcacgc atggccggga cctacaggaa cccaagacca gcgtcaacga
    11581 tccccttccc atcaattgac aacgcgccaa tcgaattccc caccacacgt gttgtcccga
    11641 aatggatcga gcagttcggt caccccctgc gtatcgaaac gctccaacaa agccgcggtc
    11701 cctcgcatca ccttcaattg caattcttcc ggatataacg ggacgacccc ataaatatta
    11761 accaccctgc cgtcactcaa ttgcaaatcg taaaactccg ggggaacgcg taccggagga
    11821 accaacaacg caaagcacat cttcgtcccc tccacgaagg gtgccggagg attcccgttg
    11881 ggcaccgaat gaccaaacgc caaccacgta tcacaagcat gagggaaacg agccaatgtc
    11941 ttgagcaaac ggatgggcca gtatttccgc tcgtctcgca actcctctag cgaccgcgga
    12001 tcgtcaaacc accccgacgg aagcatcaac atcaactcgg catatcgcag acgcttccca
    12061 tgtttcacgt catcgggaac atgcatgggc cgatcgctca tcccggtggt aaacaacgtg
    12121 aaatacggcc tattttccgt gggaagcacg acatgcacat cgacatgcac aagggaagac
    12181 accaactcat gccatacgaa cgcgggctct ccgatgcagc gagcgacatg agcttcgatc
    12241 tccactaccg tggctagagt cggcgccacg gattgcgcgg attgcttggg cggcaacccc
    12301 ctatccacct ccctcgcgtg tcgcttgccc gccatgccaa accccttggc ctgaaacgtc
    12361 caacctccga tcttcttatt gatgtgccca ccaacggctc gatctgtcaa ggccaagttg
    12421 atgcgaaatg ctaacgagac agcatgtttg ttcagggata ccggtcaagg acccggcgtc
    12481 aacgaatcac aatcgatacc tccggaaatc ccacccactt tcgccttcca cgacgatccc
    12541 acctgaaacg acttccactt ttccggatcg ctgaaagaac aggttttctc tttgcccgac
    12601 gatgcatcct gcaaacgtag ggtgtaggtt gccgtcttct ttccctctcg ctcgcagccc
    12661 tcacactcac ccggacgcga caaggtaacg gtgggccaag gcatgggatc ggtcacactc
    12721 gatccggtgt ttttttcaga acgcttcggt acccaccgtc ccacctgata cgtgcaaata
    12781 tcctcctgat ctttgcactc gcgtttttcc ttgtaggtgc catcgccctg atcgattttt
    12841 cgggtttcac aaaccggttc ctgcttctgc cttgtcactc gctccgcgga cgtcggcatc
    12901 ttgctgcatg gcgatgattc ctttttgtat tcgtacgtct cgatctccac ggcatgggtc
    12961 caggtatggc cgacgacttt gaatccagca tcttttttca ggatgctgtt gagaacgaaa
    13021 aacccgatga tcgccaggac gatcagcccg aaaatcccgc cgatggcgca tccaattccg
    13081 actttggcgc cggtttttgc tttgggtgcc tgcgctggag atggggaatg gacatggtcc
    13141 ggaatgactt gttcctgttg tagcgccgcg gctttccctc cctcgagagg cgaaccacag
    13201 tggccacaat gcttggccaa ccttccgttg gcagtctgac aggcggggca caacacgtcc
    13261 tgaccgacgt attggtggtc ttgcacggct attttttcct cttccggagg aaagtaacgt
    13321 ttggctggat cttgaggggc tccgcaattg ggacaatgtc gatgcgtcaa tccgagcagc
    13381 ttgcgcgcac cacaaaaaga gcagtcccac agcatctcat aggtcttctc gccgttcatc
    13441 accccattgt actcaggtcg aatggatgaa tgccagtgcg gaaaagctca caatctcaac
    13501 ccttcggacg tcgaagtcgc aaaacacgtc cccgtgccgg cggtggaacc aacggaggca
    13561 acgccatcac caaccgttca atccgaggaa tcgcacgaac caccatgcct tcggcatgca
    13621 actcggaaaa atcaccataa aaaacagcga caatccaata ggttgcggca aaaggcttgg
    13681 cgagaaaacc aattccctcc tgtcgaacca cgattcgcgc gcgcggacgc accaatttga
    13741 gtttcgaatt ctgccgaatt cgagcccatg ctcgaaccga caagagcaag gctcgccact
    13801 cttccactcc caaggtggga cgctccgcac gaagcctcgt ccattcacgc atcgtcgcgt
    13861 gcgataggga cttgggcagt cgcgaatcca cgagcgtttg ctcaacttcg gctcgctcct
    13921 tcaccaacaa cgcaagcaaa tccacaccca cccgcgaaca cgcttcccat accttctcga
    13981 ttcgcaacgc atcatccatt ccccgttccc cacgctgaaa agggtcggaa gagccccaca
    14041 ccaccggaga aacgtcgtca aataccgata cgttcaccgc ctccagacgt tccgcaagca
    14101 tggccaattg ctcgttgaga agcgcttggg tcaaccccac ggcaggccc
//
LOCUS       DAOSOR010000073        14132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4329_length_14132_cov_4.850749, whole genome shotgun sequence.
ACCESSION   DAOSOR010000073 DAOSOR010000000
VERSION     DAOSOR010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 14132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14132
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4329_length_14132_cov_4.850749"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="PK710_05885"
     CDS             complement(<1..719)
                     /locus_tag="PK710_05885"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 family protein"
                     /protein_id="HOT09279.1"
                     /translation="MPPLILSASRRTDIPAFFSSWWMHRVREGRVLVKNPRNPRQVLD
                     VSLRPNDVIAVVYWSRDYRRLLPHFPELEDRGLRPCFHLSLTGYGPPLEQRGPDVDTV
                     LEQFERLAKRYGPSRTVWRYDPIILGSRHHEDFHEAHFASLAERIAPLVRCGIISFLD
                     PYPSTKRGLKALEAAGYERWETPSFEQRKRLAARLVAVGKQHKLSVFACCEADLSEQI
                     APARCIDPEWIASFTSVPASA"
     gene            765..953
                     /locus_tag="PK710_05890"
     CDS             765..953
                     /locus_tag="PK710_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09280.1"
                     /translation="MKSRYLWAYRSMGADGLKGGSFALLRVTSGSYGLRHAWVRDSSA
                     VYGWKPVMTWREGLSDAQ"
     gene            943..2532
                     /locus_tag="PK710_05895"
     CDS             943..2532
                     /locus_tag="PK710_05895"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOT09281.1"
                     /translation="MLSSAATPYIVFSIGFLIMVAVLANRISGRLGIPTLLVFIAVGM
                     LAGSDGPGGIEFDNPAVANLIGVFALAYILFSGGLDTKWSKVKGVAWHAAALATLGVG
                     ISAALVGIFASYILDFTIWEGLLVGSIISSTDAAAVFAVLRSRGVSLKGDVAPLLEFE
                     SGSNDPMAVFLTLGVLGVVMDSSQKPLWLLLSFVFSMTGGLALGLAFGFLTSFILNRL
                     RLEYEGLYPVLSISVVLLTYGSCEILHANGFLAVYVAGIVLSSNDIHLKRYLMKFHDG
                     IGWLMQILLFVTLGLLVYPSQLPSVLLPAVLIATFLMFVARPVAVYSLMIRSRFTMAE
                     RTLVAWTGLRGAVPIVLATFPLLAGHPKSNMIFDIVFFVVLLSALLQGKSLMMVARWL
                     GVDEPLVDRPQSPIEFEHREGVGSEAREIDVPPSSAIVGKRVFQLGLPPGVLIVMIRR
                     DHSFIVPRGDTCVRAYDTLHILGEKSALAISEALVEQQAPHRSDDQDPPSGSSSGDAE
                     CERAVQQALPMIGSDASANAV"
     gene            complement(2649..2942)
                     /locus_tag="PK710_05900"
     CDS             complement(2649..2942)
                     /locus_tag="PK710_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOT09282.1"
                     /translation="MVAYTVCFIPSHRTIQVSHGTSLLEACHAAGLPLASSCDRSGIC
                     RACRVQIVAGEDNLCAVTRPEQEAKRQGALGNHERLACLARVQGPVTITTTYW"
     gene            complement(2943..4628)
                     /locus_tag="PK710_05905"
     CDS             complement(2943..4628)
                     /locus_tag="PK710_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09283.1"
                     /translation="MRDSFRMRTVLATAATLCLALHLGSVHRWFSVPYSLWAGWDEAY
                     ITVFAQRMLDGRWLPYVDAVSHRGPMLYWLAAILQWFAGGYSWAAMRFGALLAAQLTL
                     WLAFLIGVAFRKPLAGFLASASFVFSTVYAMEPKDGIGFNGELVAMPFVLLGALITTW
                     ALQTPGPRRESQLWFAALSGLLISLGGLCKQPTFLHILPVGLWWIVDGMAHRDKPFAK
                     GHPFQPAMAFAIGAVTPVLAVTAYFARSNAFRPFSYYLFTYNRSVYMEPVTAGYAVES
                     TFLFWREHGALLLVILSTIAWALARFFSKLEPSHHRWSTAFSSTALPTTTALHTMLAL
                     AGAIGTFRFWDHYFIPVLPWLGLLLGTMLEDSIERIRFQRPLRSNLVVLACFVGISFV
                     FRLLTTLWLDGQRRAGEFYGNPEEEPIARYVQAHSQPSESIFVWGFAPEIYVNSKRRA
                     ASRFVFTTFPAGIVPWFHWLSFEHEERLVVPGSRELLLRELESEKPALVVDVPNSIHG
                     RGIRRYPPLVSYLDQKYCFVETIVGRNGRTAHIYHRRGNAQTCQRLPPPNPSF"
     gene            complement(4698..5918)
                     /locus_tag="PK710_05910"
     CDS             complement(4698..5918)
                     /locus_tag="PK710_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09284.1"
                     /translation="MLYAFATFALVVVPGCKVYDESLLLDGASGFGAMPRGDGVGWWS
                     GTDPTTKCATASMPRAEHRPKSDPGEPIPPLVFAVRSMRLGSLDRNGNLSPTAWQDIG
                     FDLDGTCTRSPTCSTPEPIFSCKSPIPLVAGDGNFCRDNRFGELEYAISTNDDIGLRF
                     GLNDHTFNCSLCVGAYNMLFKISDYNGKPNDATVRVDIYPSPGIETLKGIDCQTQKSW
                     NADECWQKSDTWFVQSDYVNESVGQQVVGNSKLDDSTAYVRDGYVVARLRDNTRFWFP
                     DDRGVATAFPLVVQGGVVTGKLVQDQEGNWTLTDGIIAGRMDTKDAIDSFRFIGLCPG
                     DPLSSTVEGFAKAWADVLSSGAILPEVTCDALSVGIAFEASEATIGTAARVEVPDPCA
                     GVDGGVGDAEPEGG"
     gene            6478..7446
                     /locus_tag="PK710_05915"
     CDS             6478..7446
                     /locus_tag="PK710_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09285.1"
                     /translation="MIRWFSVLGCLWMVSGPVRAQSDVDPEKLRLAAEEYDAGRRAYK
                     LGDFAKAATLFENAYRDAPSAQTLRMAIRSRDEARHHARAATLCALALSTYGQDAETA
                     ALARRVLEQRAPQLVQMTVQCKPACAVLLDGLVVQDEEAQRLVLYAGEGMHTVTAGWS
                     GRRSLSKQFEGKAGESMNLFFEAPATEQPASKPQVVEPERGGSGSGKASPTLFWLGTG
                     VTAVFGGLTVWSGIDTLNNPGPDAVREACVGKGTSCPAYQQGLDNQSRTNYLLLGTGI
                     GVVSVAVIGLFFTDWNNEEASNTAVSVTPTADPSDKSLGLSTTYRF"
     gene            7722..9224
                     /locus_tag="PK710_05920"
     CDS             7722..9224
                     /locus_tag="PK710_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT09286.1"
                     /translation="MGEPSEVGISEVTNQRVERYELLAELAAGGMATVYLARLGGAGG
                     FQRLYAIKKLHPHFAHEREFVEMFLDEARLAARIHHPNVVPILEVGQSQSGFFLVMEY
                     IEGETLARLLGRTSLREERIPPRVGAKIVLDVLAGLEAAHSLVDDFGKPLHIVHRDVS
                     PQNVMVGLDGTSRITDFGVARAATRISTTRAGQLKGKVGYMAPEQARGDDIDSRADVF
                     SVGVILWETLAGRRLFKNKPDAPDTHTLQRLLHEPIPTLRDIAPGTPEAIERVCFSAL
                     ERDPDRRVASCGAFAEALEAAMMQSCGVASAREVAAFVESVAGPELEQQREAVRNWVS
                     RSDINVLDPHALHEPSAPTPSRLTSPFVLQNPTPKTPPPRTTNLPLILVSVAFLLLAI
                     AFVGVVILFLRETSPTHNASIADSSAYPAASSTSATETAAPSPCVSASVHPPDVQPAE
                     TSSVTRSTPKRDRSPSTADAGKSETTTNVEEPVKPTTDSLDDLDTNPYRP"
     gene            9433..10407
                     /locus_tag="PK710_05925"
     CDS             9433..10407
                     /locus_tag="PK710_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09287.1"
                     /translation="MNDKRNDGSDHTEAEELENNDASASVTDDEQSQANSSPSGSDAK
                     DVKVAENKEQISEANNEDDEDEDGDEDDEDEDGDEDEDGDEDEDDEDEDEDDEDEDDE
                     DEDDEPKKAKASSKVVAKSAPAKSSLNKKAAKPITKMPSEIPLPPRNNEIVTGLVVAI
                     GGYIAFWLGWSIYSSSIEQKNVNFFSNSAWAFLVAYFLIAVAATFFVRFERRDPKDEK
                     NKKPKLERQGLLLASLVCGPFYVAYWWKGIELWEKMYAQERPGMWVIFVAFFAFVTFG
                     IGYGLRPPSENEELQRRIPGRRVLLMVMTPFAIVYGMIYLAAFYPPWP"
     gene            complement(10479..11690)
                     /locus_tag="PK710_05930"
     CDS             complement(10479..11690)
                     /locus_tag="PK710_05930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOT09288.1"
                     /translation="MNPKTSAQSGEHPVSLAEDLSITAFALLEGLCSFVFVVGRDGTI
                     RYATPSVFRQTGVEPSKCVGHPFVAAPFWSEATAQEDIAVGLRDAFQGRKADFLVDIS
                     TLSKRITVNFDCKPLRSAEGIIEAILVEGHDVTDRRLLERKLRDSHFHWRTIADCAVD
                     WEFWLHPAGYFIYNSPSCQRITGYSIDELTQNRITINSMAHPDDRKRVTELLTAAYSG
                     TTGHSQRFRIVRRDGSARSVSMSWQPVQGDDGRFLGIRGSVRDVTDLADTEAELQRLL
                     EAYRILAMHFPKGLVALLDSELRFVVADGPAFQWVEIDPKRIIGKRFDQVFEPALLER
                     VGPLFFRARAGHEVSDILEYQNATWLVHLTPILGPDRGVRHVIASAVLSRGSAWDALE
                     EKPTSPAHSIG"
     gene            complement(11816..11950)
                     /locus_tag="PK710_05935"
     CDS             complement(11816..11950)
                     /locus_tag="PK710_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09289.1"
                     /translation="MSAVLGHDAFETSPLMTIVAPHHRPTKSALANFLACGLLRPRQW
                     "
     gene            12451..13665
                     /locus_tag="PK710_05940"
     CDS             12451..13665
                     /locus_tag="PK710_05940"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOT09290.1"
                     /translation="MGKLQGKAVVAQGGGPTAVINQSLVGAVLEARKYDHITHVYGAR
                     HGVRGIVEEDFLDLSQATTHNLEMVAHTPSSGLGSTRDKPDEEYCAKIFQVLRAHGVR
                     YFYYAGGNDSADTCRIVNENANRAGYELRVIHIPKTIDNDLRVTDHCPGYGSAAKFVV
                     SAFAGVNLDNAALPGVYIGIVMGRHAGFLTAASVFARKYPDDGPHLVYVPERVFDKEK
                     FLAAVDRVYSKFGRCVIAVSEGIVDASGQPVILGLRGDTERDAHGNVQLSGSGALGDG
                     LSDLIKEKLKIKRVRSDTFGYLQRSFLSVVSTADANEAREVGEKAVQYSVWNNVDGSV
                     AIRRTGDYSVDYFLTELESVAKHTKHMPEEYLEGEADVSTAFKNYARPLIGNFPHYER
                     IAAPVVDKILGK"
     gene            complement(13674..13883)
                     /locus_tag="PK710_05945"
     CDS             complement(13674..13883)
                     /locus_tag="PK710_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09291.1"
                     /translation="MSDPVVSPSTSIEDLVQWIPEAVGYLRDKGIRCIRCGEPIWGTL
                     EEAAKQKGFSEEQIARFVADLNALR"
     gene            complement(13941..>14132)
                     /locus_tag="PK710_05950"
     CDS             complement(13941..>14132)
                     /locus_tag="PK710_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09292.1"
                     /translation="ACRPITTAHNEIFRFYQVGFRCCTNTKKNSDGVADRYTFRLSEW
                     RKRASLRPEGGPFERPPQP"
BASE COUNT         3024 a         3851 c         4236 g         3021 t
ORIGIN      
        1 atcgccgaag caggcacgga tgtgaacgag gcgatccatt cgggatcgat gcagcgcgcg
       61 ggcgcaattt gttcggaaag atcggcttca caacaggcaa agaccgatag cttgtgttgc
      121 ttgcccacgg cgacgagacg cgcggcgaga cgttttcgct gttcgaacga aggggtttcc
      181 catcgttcgt atcccgctgc ctcgagggcc ttcaaccccc gtttggtcga gggatatggg
      241 tcgaggaaac tgatgatgcc gcagcgcacc agaggtgcga ttcgctcggc cagtgaagca
      301 aaatgcgctt cgtggaagtc ctcgtgatgc cgactgccca ggatgatggg atcgtatcgc
      361 catacggtgc gcgaggggcc gtatcgtttg gcgagtcttt cgaattgctc gaggaccgta
      421 tcgacatcgg gacctcgttg ctccagcggg ggcccatagc cggtgagaga gaggtggaaa
      481 caagggcgta acccacggtc ctccaattcg ggaaaatgcg gcaacagcct tcggtaatct
      541 cgagaccaat acacgaccgc gatcacgtca ttcgggcgca gagacacgtc cagcacttgt
      601 cgcgggttgc gaggattttt cacaagaacg cgcccctccc ggacgcgatg catccaccat
      661 gaggaaaaaa aagccggaat atctgttcga cggcttgcgc tgaggatgag cggtggcacg
      721 ttgctcacgt tgacgcgatc agaaacgaag agcaactgct tgcgatgaaa agccggtatc
      781 tatgggcgta tcgttcgatg ggagcggatg ggttgaaagg tggttcgttt gccttgcttc
      841 gcgtaacgag cggcagctat ggtttgcgcc atgcatgggt tcgtgactct tcggccgtgt
      901 atggctggaa acccgtaatg acctggcggg agggtttgtc ggatgctcag tagcgcggca
      961 acgccctaca tcgtgttttc catcggcttc ctcatcatgg tggccgtact cgccaatcga
     1021 atttccgggc gcttgggtat tcccacgttg ttggttttca tcgccgtggg gatgcttgcg
     1081 ggctccgatg gccctggggg aattgagttc gacaaccccg cggtcgccaa cctcatcggc
     1141 gtgttcgcat tggcatatat tttattttcc ggcggcctgg acaccaagtg gagcaaggtc
     1201 aaaggggtgg catggcatgc ggccgccctg gccacgttgg gagtcggcat ctccgcggct
     1261 ttggtcggca tatttgcctc ctacatcctc gacttcacga tctgggaagg cctgctggtc
     1321 ggttccatca tctcatcgac cgatgctgcc gccgtcttcg ccgttcttcg ctcacgaggc
     1381 gtaagtctca aaggggatgt ggcgccgctt ttggaattcg aatccggaag caacgacccc
     1441 atggctgtat ttctgacctt gggggtcctc ggtgtcgtca tggactcctc gcaaaaaccc
     1501 ctttggctct tgctttcctt cgtgttcagc atgaccggcg ggctggcgtt gggactcgct
     1561 ttcggattcc ttacctcctt catcctcaac cgacttcgtc tcgaatacga agggctctat
     1621 ccggtgctca gcatcagcgt cgttctgttg acctacggaa gctgcgaaat ccttcacgcc
     1681 aacggctttc ttgccgtgta tgtggccggc atcgtgctca gcagcaacga cattcacctc
     1741 aagcgctatc tgatgaagtt tcacgatggc atcgggtggc tgatgcagat ccttttgttc
     1801 gtcaccctgg gccttctcgt atacccctcg caacttccca gtgtcctgct gcccgcagtg
     1861 ctcatcgcca ctttcctcat gtttgtcgcc cggcccgttg ccgtgtactc gctgatgatt
     1921 cgaagccgat tcacgatggc cgaaagaacg ttggtggcgt ggaccgggct gcgcggcgcc
     1981 gttcccatcg tcctcgctac cttccccctg ctggctggac acccgaagtc caatatgatc
     2041 ttcgacatcg ttttcttcgt cgtcctgctg tccgccctgc tacagggcaa aagcctcatg
     2101 atggtggcta gatggctcgg ggtcgatgag cccctcgtcg atcgaccgca atcccccatc
     2161 gaattcgaac atcgagaagg cgtgggaagc gaagcccgtg agatcgatgt gccgccttct
     2221 tccgccatcg tgggcaaacg ggtctttcaa ctcggactgc cacccggtgt gctcatcgtc
     2281 atgatccgaa gagatcattc cttcatcgtg cctcgaggag atacctgcgt gcgcgcgtac
     2341 gatacgctgc atatccttgg ggaaaaaagc gcccttgcga tatccgaagc gctcgtcgaa
     2401 caacaggctc cccatcgaag cgatgatcaa gatccccctt cgggcagttc ttccggtgac
     2461 gccgaatgcg aacgagccgt gcagcaggca ttgcccatga tcggatcgga cgccagcgca
     2521 aacgctgtct gatgtccacg aggccatctg tggcaaggcc ttggaaagcg tcgctttcat
     2581 cctcccggca ttcaaaaaaa tattctgtaa tatcaactag ttgccttgtg tttcggaaca
     2641 cgggccgatc accaataggt ggttgtgatg gtgaccggtc cctggacgcg cgccaggcaa
     2701 gcgagtcgct catggtttcc gagtgctccc tgccgtttcg cttcttgctc ggggcgagtt
     2761 acggcacaaa ggttgtcttc tcccgccacg atctgaactc gacaagcgcg acagatgccg
     2821 gatcgatcac aagaggaggc gaggggcaat cccgcggcgt ggcacgcctc gagcaaggat
     2881 gtgccgtggg atacttggat cgtgcgatgg gaagggatga aacaaacggt atacgcgacc
     2941 attcagaaac tggggttggg tggagggagg cgctgacagg tctgcgcgtt gccgcgtcga
     3001 tggtagatat gtgccgtgcg accattgcga cccacgatgg tttcgacaaa gcaatatttt
     3061 tgatcaaggt acgagacgag ggggggatag cggcgaatcc cgcgtccgtg gatggaattg
     3121 ggaacgtcga cgacgagtgc cggtttttct gactcgagtt ctcgcagcaa tagttctctc
     3181 gatcctggaa cgacgagtcg ttcctcgtgt tcgaaggata gccaatggaa ccacgggacg
     3241 atccccgcgg ggaaggtggt gaaaacgaat cgggaggcgg ctcgacgttt ggaatttaca
     3301 taaatttcgg gggcaaatcc ccatacgaag atgctctcgg agggctggga atgcgcctga
     3361 acgtaccgag cgatcggctc ctcttcgggg ttaccgtaga actctccggc tcgccgctga
     3421 ccatcgagcc ataaggtggt gagaagacga aagacgaaac tgatgccaac gaagcatgcg
     3481 agtacgacta gattcgatcg cagggggcgt tggaagcgta ttcgttcgat ggagtcttcg
     3541 agcatggtgc cgagcagcaa gccgagccag ggcaacaccg ggatgaagta atggtcccag
     3601 aatcggaagg taccgatcgc tcccgcgaga gcgagcatcg tatgcagtgc cgtggtggtg
     3661 ggcagagcag tcgatgaaaa cgcggtagac cagcggtgat ggcttggttc gagtttggag
     3721 aagaaacgag ccaacgccca tgcgatggtc gaaaggatga caagcagcag cgcgccgtgt
     3781 tcccgccaga aaaggaaagt ggattccacg gcatagcccg cggtaacggg ctccatgtag
     3841 acgcttcggt tgtaggtgaa caggtagtag gaaaagggtc tgaaagcgtt agagcgagcg
     3901 aaataagcgg tgaccgcgag gacgggagtc acagctccga tggcgaatgc catggcgggc
     3961 tggaagggat gtcctttggc gaagggctta tcgcgatggg ccatgccatc gacgatccac
     4021 catagtccta ctggtaaaat atgtaggaaa gtcggttgct tacaaagacc tcccagcgag
     4081 atcagaagac cgctgagtgc ggcgaaccat agctgtgact ctcggcgtgg gccgggtgtt
     4141 tgcagagccc aggtcgtgat caacgcgcca agcaagacga acggcatggc gacgagttca
     4201 ccgttgaagc caatgccatc cttgggctcc atggcgtaga cggtggagaa cacgaaagag
     4261 gcgcttgcca gaaatcccgc tagcggcttt cggaatgcca cgccgatgag aaaggccagc
     4321 caaagcgtga gctgggcggc gagcagggct ccgaaccgca tcgcagccca cgagtagccg
     4381 ccggcaaacc actgaagtat cgccgcgagc cagtacaaca tcgggccccg atgactgacg
     4441 gcgtcgacat aaggaagcca tcggccatcg agcatgcgct gggcgaagac cgtgatatag
     4501 gcttcgtccc agccagccca aaggctgtaa ggtacggaaa accatcgatg aacgctgcct
     4561 aagtgcaacg cgagacaaag ggtggcggcc gtcgccagta cggttcgcat gcgaaaagaa
     4621 tcacgcatgg gctgaccgtt tcgcgcaggt ggtccgcgat atttcaaaga ggttgcggcg
     4681 aggggcggcc acgggcttca tccaccctcg ggctccgcat caccaacgcc gccgtcgacg
     4741 cctgcacatg gatccggtac ctccacgcga gccgccgtgc cgatcgtcgc ctccgaagct
     4801 tcgaacgcga tgccgaccga aagggcatca catgtgacct cggggagaat ggcaccgctc
     4861 gacagcacat ccgcccatgc cttggcgaac ccttcgaccg tggatgacag ggggtctccg
     4921 gggcacaagc cgatgaatcg aaaactgtcg atggcgtctt tggtatccat cctgccagcg
     4981 atgattccat cagtcagcgt ccagttgcct tcctggtcct gaaccaattt gcctgtcacg
     5041 acgccccctt gcaccaccag agggaatgcg gttgcgacgc ctcgatcgtc gggaaaccaa
     5101 aacctcgtgt tgtcacgcaa tcgagcgacc acgtagccat cgcgcacata ggcggttgaa
     5161 tcatcgagct tggaattacc gacgacttgt tgtccgacgg attcgttcac atagtcggat
     5221 tggacgaacc acgtgtcgct tttttgccag cactcatcgg cattccaact cttttgcgtt
     5281 tggcaatcga ttccttttag cgtttcgatg cccggggaag gataaatatc gacacgcacc
     5341 gtggcgtcgt tgggcttgcc attgtagtcc gatattttga acaacatatt gtaggcgcca
     5401 acacacagcg aacagttgaa cgtgtgatcg ttgagtccaa agcgaaggcc aatatcatca
     5461 ttggtcgaaa tggcgtattc gagttcgccg aagcggttat cgcgacagaa gttgccatcg
     5521 ccggctacga gagggatggg ggatttgcag gagaatatgg gttcgggggt gctgcacgtg
     5581 ggcgagcgcg tacacgttcc atccaggtcg aagccaatgt cctgccacgc ggtgggactc
     5641 aggttgccgt tgcgatcgag agaacccaga cgcatggaac gaacggcaaa aaccaagggg
     5701 gggataggtt ccccaggatc gcttttcggt cgatgttccg cacgaggcat cgaagcggtg
     5761 gcgcatttgg tagtcgggtc ggttccggac caccatccaa cgccatcgcc tctgggcatc
     5821 gcaccgaatc cgctcgcccc atccaatagc agggattcgt cgtaaacctt gcaacccggc
     5881 accacaacca gcgcgaaggt ggcgaaggca tagagcatcg ctcgattcat aacggacctg
     5941 gagaatacct tctgggctcc gctgggcaaa cgtcttggtt tgtgtgggtt aatgaaggat
     6001 aagtgctcac caaggagccg cgatgaacga tcatggtcac ggccatagaa tacggtagcg
     6061 gtcacggtgc atagcacgcg gctgcggtca cggtcaccca cgtggggacc gtcgcatctg
     6121 gctttgcggg tccgccttgc cgcggggcaa tgggtccacg aggagagcga cggcctggcg
     6181 cagcttctat gcgttgcggt acctatcgaa ctggtgcaac tggttttgtc gacagcgtgg
     6241 caccgctgcc gtttctcatg tgccgtgacc gttggatgtg ttctccctgc aggaacggtt
     6301 ttcgtatcgt acccattcag ctcctgtatg ttggggtgct ggggtatgga acttgaattc
     6361 gaggactcga cgttggacga cggtcaaggt acagaagcgg ttgttggaag acgaaagagg
     6421 caagatggtg gggttgattt cattggacat tgggtttcgt gaatgaagct tcgtcgcatg
     6481 attcgatggt tcagcgtgct gggttgcctg tggatggtgt caggacctgt gcgcgcgcaa
     6541 tcggatgtcg acccagagaa actccggttg gcagcggaag aatacgatgc ggggcgaagg
     6601 gcgtataagc tgggggattt cgccaaagcg gcgacgttat ttgaaaacgc gtatcgcgat
     6661 gcccccagtg cgcaaacgct gcgtatggcc attcgttccc gagatgaggc gcgtcatcat
     6721 gcaagggcag ccacgttgtg tgcgctggcg ctttccacct acgggcaaga tgccgaaacg
     6781 gcggccttgg cgcgtcgtgt tctcgaacag cgcgcaccgc aattggttca aatgacagtg
     6841 cagtgcaaac cagcctgtgc cgtgttgctc gatggtctgg tggtgcaaga cgaagaggcg
     6901 cagcggctcg ttctttatgc gggggaggga atgcatacgg taaccgcggg ctggagcggc
     6961 aggcgaagcc tttccaaaca attcgaaggc aaggcgggcg aatcgatgaa cctttttttc
     7021 gaagcgccag cgaccgagca gcccgcatcg aagccacaag tcgttgagcc ggagaggggc
     7081 ggttccggtt cgggaaaggc ttctcccacg ttgttttggc ttggtacggg ggtgactgcc
     7141 gtatttgggg ggctgacggt atggagtggc attgacacac tcaacaaccc gggccccgat
     7201 gcggttcgcg aagcttgcgt gggaaaagga acgagttgcc ccgcgtacca gcaggggctc
     7261 gataatcaat ccaggaccaa ctacctgctt ctcggcacgg gcattggcgt cgtgtccgta
     7321 gccgtcatcg gtttattttt taccgattgg aataacgagg aggcgtcgaa cacggccgta
     7381 tcggtcacac cgacggccga cccttctgac aaaagccttg ggttgtctac aacctatcga
     7441 ttttaaagcg tttttagctc tggcgcccga gtcttcgatt catcgagcgc gggttcgcaa
     7501 ggaggggtat tggcggaaac tcgacaaact ggcaaagacc cggtagttgc atggactgcc
     7561 ctggattgtc ggggattggg tggttggagc gaagtgaggt ttggcaggga tggggtcggg
     7621 ttcgggatgg aggaagcggc tttttcggtt gtatttgctg ccgttccggc tcatgaaaac
     7681 ctaatcatgg tagccaatcg tccgtatcga taggagtgcg tgtgggcgag ccgtcagagg
     7741 taggaatcag cgaagttacg aatcagcgtg tcgaacgata cgagctgctg gcggagcttg
     7801 ccgcgggcgg catggcgact gtgtatttgg cgaggcttgg tggcgctggc gggtttcaac
     7861 gcctttatgc catcaaaaaa cttcatccgc atttcgccca tgaacgcgaa ttcgtggaga
     7921 tgtttctcga tgaggcgagg ctcgccgctc gcatccatca ccccaacgtg gttcccattc
     7981 tcgaagtagg gcaaagccaa agcggttttt tcctcgtcat ggaatatatc gaaggcgaaa
     8041 ccctagcccg attgctaggg cgcacgtcgt tgcgagagga acgaattccc ccgcgggtcg
     8101 gtgcgaaaat cgttctcgac gttcttgcgg ggctcgaagc ggcccatagc ctcgtcgatg
     8161 atttcggaaa gccgttgcac atcgttcatc gcgacgtttc gccgcagaac gtcatggtgg
     8221 ggctggatgg gacctcgagg atcacggatt ttggtgtagc gcgggcagcc acgaggattt
     8281 ccacgacacg cgcgggtcaa ctcaaaggca aggttggcta catggcgcca gagcaagcgc
     8341 gaggcgacga tatcgattcg cgggcggatg ttttttccgt gggggtcatt ctttgggaga
     8401 cgctggcggg gcgacgattg ttcaaaaaca agcccgatgc gcccgatacg cacaccttgc
     8461 aacgtttgct ccatgagccc attccgacgc ttcgcgacat cgcccccggt actcccgaag
     8521 cgatcgaacg cgtatgtttt tcagctttgg aaagagatcc cgatcgtcgc gtggcctcct
     8581 gtggtgcttt tgccgaagct ctcgaagcgg ccatgatgca aagctgcggt gtggcgtcgg
     8641 cgcgcgaagt ggcggctttc gtcgaaagcg tggccggacc cgaactcgaa cagcaacggg
     8701 aagccgtgcg caattgggtc tcccgaagcg atatcaacgt gctcgacccc cacgccttgc
     8761 acgaaccgtc cgcgcccaca ccttcccgtc tcacaagccc cttcgtactc cagaatccca
     8821 cgccaaaaac tcccccgccg cgaacgacca acctgccttt gattctggtc agtgtcgcct
     8881 tcctcttgct cgccatcgct ttcgtaggtg tcgtgatcct gttcctgcgt gaaacctcgc
     8941 caacccataa cgcttcgatc gccgactcct ccgcctatcc tgcggcttca tcgacctcag
     9001 ccacggaaac ggcagcccct tccccttgtg tctccgcttc cgttcatccc cccgacgttc
     9061 aacccgcgga gacgtcatcc gttactcgat ccactcccaa acgagaccgc tccccctcca
     9121 ccgcggatgc cggcaagtcg gaaacgacga ccaacgtcga ggagccggtg aaaccgacca
     9181 ccgactctct cgacgacctc gacaccaatc cttatcgacc gtgaatgacg cgaatcgtat
     9241 cgaaatttgc agttttctat gtcggattgc aggcggaggg gttgctactg cccctggccg
     9301 tgctcccgct atcgaactcg tgctagccac ccatgccatt cgcgttgtag gcagactctt
     9361 gcagtcgtgc tacacgtgcg tcttcccgtg gaacttcgac cccgcggagc ctgtggcatg
     9421 taggagtgtc ggatgaacga caagcgcaac gacggttccg accatacgga agcagaggaa
     9481 ctcgaaaaca acgatgcctc cgcatcggtg acagatgatg aacaatcgca agccaattcg
     9541 tctccctccg gctctgacgc gaaagatgtg aaagtagctg aaaataaaga acaaattagt
     9601 gaggcgaaca acgaagacga cgaagacgag gacggcgacg aagacgacga agacgaggac
     9661 ggcgacgaag acgaggacgg cgacgaagac gaggacgacg aggacgaaga cgaggacgac
     9721 gaagacgagg acgacgaaga cgaggacgac gagccgaaaa aggccaaagc atcctcgaag
     9781 gtcgttgcca agtcagcccc cgcgaagtcc agtttgaata aaaaggcagc caagccaatc
     9841 acgaagatgc cgtcggagat ccctcttccg cctcgcaaca atgagattgt cacggggttg
     9901 gtggtggcca ttggcggcta tattgccttt tggcttggct ggagcatcta ctcgtcttcc
     9961 atcgagcaaa agaacgtcaa cttttttagc aacagcgcct gggcatttct tgtcgcttac
    10021 tttctcatcg ctgtggcggc gacgttcttc gtgcgcttcg agcgtcgaga tccgaaggat
    10081 gaaaagaaca aaaaaccaaa gctagaacgt cagggattgc tgctcgcatc gctggtatgc
    10141 ggtccttttt atgtggcgta ttggtggaag gggatcgagc tttgggaaaa gatgtacgct
    10201 caggagcggc ctgggatgtg ggtgattttc gttgcgtttt ttgcgttcgt gacgtttggc
    10261 atcggctacg gattacgtcc accctcggag aacgaggagt tgcaacgacg tatcccgggg
    10321 cgccgtgtgc ttttgatggt gatgacgcca tttgccatcg tttatgggat gatttatctg
    10381 gcggcgttct atcccccatg gccgtgagcg gggtgatttc atgaggggtt aggcccttcg
    10441 gatttggaag agcgggggtc gagaggctgg cttggatgct agcctatcga atgagcgggg
    10501 ctggttggtt tttcttcgag ggcatcccag gcggagccgc ggctgagcac cgcggaagcg
    10561 atgacatggc gaacgccgcg gtcaggaccc aggatgggag tgaggtgaac gagccaggtg
    10621 gcattttggt attcgaggat atccgatacc tcatgaccgg ctcgtgcgcg aaagaatagg
    10681 gggcccacgc gttcgagcag ggcgggttcg aatacctggt cgaagcgttt gccgatgatt
    10741 cgcttgggat cgatttcgac ccattgaaaa gcgggtccgt cggccacgac gaatcgcaat
    10801 tcgctgtcca acagggcaac gagtcctttg ggaaagtgca tcgccagtat tcggtaggct
    10861 tcgagcaagc gttgcaattc cgcttccgta tcggccaagt ccgtgacatc ccgaacactg
    10921 ccgcggatgc caagaaaccg gccatcgtct ccttgcaccg gttgccaaga catggacacg
    10981 gatcgggcac tgccatctct gcgtacgatc cggaaacgtt ggctatgtcc tgtcgtgccc
    11041 gaataggccg cggtaaggag ttccgtcact cttttgcggt cgtcgggatg agccatggag
    11101 ttgatggtga ttcggttttg ggtcaactca tcgatgctgt atccggtgat tcgttggcac
    11161 gaagggctgt tgtaaatgaa gtatcctgct ggatggagcc agaattccca gtccacggcg
    11221 caatccgcaa tcgttcgcca gtgaaagtgc gaatcgcgca gcttgcgttc gagcagacga
    11281 cgatccgtga cgtcgtggcc ttcgacgagg atggcttcga tgattccttc ggcgctgcgc
    11341 aagggtttac agtcgaaatt cacggtgatt cgtttggaaa gcgttgaaat atccacaaga
    11401 aagtccgctt tccgtccctg aaaggcatcg cgtaatccca ccgcgatatc ctcttgtgcc
    11461 gtcgcttcag accaaaacgg agctgccacg aaggggtgac ctacgcattt cgagggttcg
    11521 actccagtct gtcgaaaaac ggatggcgtt gcatagcgaa tcgtcccgtc ccgacctacg
    11581 acaaacacga aggagcatag cccctcgaga agagcgaatg cggttatcga caggtcttcg
    11641 gcaagcgaca cgggatgttc accggattgt gctgacgttt ttggattcat ggaacgtgat
    11701 gggcctttcg agttcacgac gcgcatcacg ggagccaacg ggagcatcgc ccgatgactt
    11761 ccctttttcg cccacgggtt tttgaaaagg cgaatgcctt tcaagaaccg tgagttcacc
    11821 attgacgagg tcgaagcaaa ccacaagcca agaagttggc aagggcggat ttcgtgggac
    11881 gatgatgcgg tgcgacaatc gtcatcaggg ggcttgtctc gaacgcatca tggcccaaga
    11941 cagcagacat gctccaacta cgtaagcgag tgaatcgcat tcccaggttt acctttcatg
    12001 ggtgtgtcgt taccaagcat cgttcgaaga gttgtcgcgg tttgtgccag cccatcaatg
    12061 gaatcggagc ctgtgggcga atcgctccgc atagcatggt gagtaaaatc cagctcggta
    12121 ttgccgcgca caaacgagag gtggaacgtc atcgagccag cgaaaacgca agctagttcg
    12181 aggtgccaac aaccggcgag atagggacta tcggtcggct aggatgctgc caagcgcggg
    12241 atgcgcatgg cgtgtggggg ggtgctgtct ttggtttcac cgaaatcccg atcgcacctg
    12301 ccatcgatgt ttcttcgcgt gtggagaacg actagatttt tactgttttg agcggtagct
    12361 tgccgcaaac ggtaacgcca acctgaggtg ttttggcata cccgggtttc atgtccttcg
    12421 gtcccctttt caaacaaagg aaatcatccc atgggaaagc tccagggaaa agctgttgtt
    12481 gcccaaggtg gtggccccac cgccgtcatc aaccaaagcc tggtcggcgc ggttctcgaa
    12541 gcgcgtaaat acgaccatat tacgcatgtt tatggtgcgc ggcacggcgt tcgtggaatc
    12601 gtcgaggagg actttctcga cctttcccaa gccacgacac acaacttgga gatggtggcg
    12661 catacgcctt cgtcaggtct tggttccact cgcgacaaac cggatgaaga atactgcgcc
    12721 aaaatctttc aagtgctgcg cgctcacggc gttcgatatt tctattacgc cggcggcaat
    12781 gattccgccg acacttgtcg tatcgtcaat gaaaacgcca accgcgcagg atatgaactg
    12841 cgcgtcatcc atattcccaa aacgatcgac aatgaccttc gcgtcaccga ccactgccca
    12901 ggctatggtt ccgccgccaa gttcgtcgta tccgcgttcg ctggcgtgaa cctcgacaac
    12961 gccgctttgc cgggcgtgta catcggcatc gtcatgggac gacacgccgg ctttctcacc
    13021 gccgcatccg tgtttgccag gaaatatccc gacgacggac ctcatctcgt ttacgttccg
    13081 gaacgcgtct ttgataaaga gaagttcctc gccgccgtcg accgcgtgta ttccaaattc
    13141 ggtcgatgtg tcatcgcggt ttcggaaggg atcgtcgatg ccagcggaca acccgtgatc
    13201 ctcggccttc gcggcgatac cgagcgcgat gctcacggca acgtgcagct atccggctct
    13261 ggcgctcttg gcgatggtct ttccgacctc atcaaagaaa agttgaagat caagcgcgtt
    13321 cgctccgata cgttcggcta tctacagcgg tcttttctca gtgtcgtatc caccgccgat
    13381 gccaacgaag ctcgcgaagt aggcgaaaaa gctgtccaat attccgtgtg gaataacgtc
    13441 gatggatcgg tcgccattcg tcgaaccggt gactattcgg tcgattactt ccttacggaa
    13501 ctcgaatcgg tggcgaaaca taccaagcat atgcctgaag aatatctcga aggtgaagcg
    13561 gacgtatcca cggcgttcaa gaattacgcc cgcccgctca tcggcaactt cccgcattac
    13621 gagcgcattg cagcccccgt ggtggataag atcctcggca aatgaccgtt cgcttaccgt
    13681 agcgcattca agtcggccac gaaccgggcg atctgttcct ccgaaaagcc tttttgtttc
    13741 gcggcttctt ccaacgtgcc ccaaatgggt tctccacagc ggatgcagcg aatacccttg
    13801 tctctcaagt agccgactgc ctcgggaatc cattggacaa ggtcttcgat gctggtgctt
    13861 gggctcacga caggatcaga catatcaact ccagggaaag aaccagatga tcgtcggaga
    13921 ctccgggcgc aggaaggatt ctacggttgg ggaggacgct cgaacggacc gccttccgga
    13981 cgcaacgaag cccgttttcg ccattcgctc aggcgaaatg tgtagcggtc cgccacgcca
    14041 tcggagtttt ttttcgtgtt cgtgcagcaa cggaatccca cctggtaaaa acgaaaaatc
    14101 tcgttgtgcg ccgtggtgat ggggcgacag gc
//
LOCUS       DAOSOR010000074        14086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4347_length_14086_cov_4.396836, whole genome shotgun sequence.
ACCESSION   DAOSOR010000074 DAOSOR010000000
VERSION     DAOSOR010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 14086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14086
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4347_length_14086_cov_4.396836"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..458
                     /locus_tag="PK710_05955"
                     /pseudo
     CDS             57..458
                     /locus_tag="PK710_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
     gene            501..977
                     /locus_tag="PK710_05960"
     CDS             501..977
                     /locus_tag="PK710_05960"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09293.1"
                     /translation="MRGWGASSDAHHIVAVEPEGKGMARAMRLALSDSHIAPEEMDYI
                     SAHGTSTPMNDRLETLAIRDVFGKHANDLLVSSQKSMIGHAMGGAGAIEAVVTVLTLA
                     HGVATPTINLQTPDPDCDLDYVPHEARKKPIRAAMSNSFGFGGHDSAIIFAQPNGL"
     gene            1022..2122
                     /locus_tag="PK710_05965"
     CDS             1022..2122
                     /locus_tag="PK710_05965"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOT09294.1"
                     /translation="MRALVLGGTGFVGMNVTRALACRGHDVIATRRARANTLFARKLG
                     AKLIVADLDDVDSLTQAMKGRQVVFHCAGFYPRYSLNIDEQVSLAQRTTRHVIEAARR
                     ACIDRLVVTSSVATIGPPRQGRVLSNESDEMSADARDSVYFASKEAIERVALETRDLD
                     VVVTCPGGIVGELDVKAGTGFIIVALAHRLLPFYVQGKTNIVDADDMAEGHLAAAERG
                     RKGQRYILGGHNTTIKDLLLGIGDELGIRFTSWPMPLAIAKPMATFDEMRCAARGKGE
                     RPFIPREFVDIVRFGQWVDTERSMSELALREPTPLHITIQKACAWYERYRYIPRRLES
                     RSVGESPSLPRIFAASSHSKSTDISSESRRTP"
     gene            2119..2370
                     /locus_tag="PK710_05970"
     CDS             2119..2370
                     /locus_tag="PK710_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOT09295.1"
                     /translation="MTAPSADILRNDVDEIIRRIRPDFKGEIKVTDRLREDIGLDSLH
                     AMELLSAVTEKYELDVDIEEVQEVKTVGDVVQFLTTAMA"
     gene            2405..3706
                     /locus_tag="PK710_05975"
     CDS             2405..3706
                     /locus_tag="PK710_05975"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOT09296.1"
                     /translation="MKIQFIYPAFERHAQSHPELLDAVPCDEYLGGPSLAIANLAAVT
                     PAGYEMAYCDDRVTPFSLDLDADIYALSFFTPAATRALEIGDMIRGAGKKVVAGGIFP
                     SALPDVAQQHFDSIVIGEGEGVWQRLLEDAAAGRLAPRYQQQEPFDLATLPPPRIDLY
                     IGAENDRFHPDDYPLQTSRGCPLRCDACVVPCVMGKKLRLVPQETILRAVQDLASHGK
                     RAAVTEDTSFFFFSGARRHFREFLKALRDDPRPRGEKVSYIGITMPMILALDPTILAD
                     ISEAGINRFYLVCGFDPITRKAFGSGDQESLDKAVEAIQRCKDNDIEPYTSLLVGNDT
                     DDEGVFDRILSFTEKADVPKTEFAIFTPYPGTPAWEQMQAEGRIIDRTWKHYNDANVV
                     FRPKQMSPERLLQGYLDLWRDFYKSKTFLASLEHGDRTIQF"
     gene            3725..5335
                     /locus_tag="PK710_05980"
     CDS             3725..5335
                     /locus_tag="PK710_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT09297.1"
                     /translation="MPGKTNLFTPILEAFRQIGPRLATSGRTQEEQEVVASILREVNR
                     YCRKEVDGLAIDRRGALGQEMLDAVAERGWFGLTIPEEYGGAGLSMSAATRIATEICK
                     YNGSLATCVALHSGLALYGLIHLASKDLQSRYLPEIAAGQRIAAFCATEPNAGSDIGS
                     MRTLLYEQDGIFRLRGSKSYVTNGGFAGVLTVIASSPGLGGARGGHTMVVIDPSWTGV
                     HKQAEENKLGLKGSSTITIDFDDVEVPRDHVIGEVSKGLDLAHEALTWGRSFMAAGCL
                     GSAMAAVQEAREHIAGRVQFGRTLDQFPLVREQMAAAMADVYTVETVLRLVCDVFDSR
                     SGDIALDSTVAKVLGSERAWNVIDRGLQLMGGAGFIEERGMPRRLRDVRVTRIFEGAN
                     DVLRLHLASATLGWPRAGLQEFPEVSPLVPAPLRTEAERFDEMLREVGVCLASIQKQY
                     GFKLFQRQSLQSVMADAIMATYGMMAVLVRAAASVQDQDTPETCVELATAKLACKRLE
                     SEARLALQTMTRGADDEALTLANAVLAQ"
     gene            5372..5671
                     /locus_tag="PK710_05985"
     CDS             5372..5671
                     /locus_tag="PK710_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOT09298.1"
                     /translation="MSVVVTIEHFGLIRETMPSPQEKIVVPVGMTVRELFQHLIERYG
                     DSYRDALLAPQGQPLPNVVLTLDGEQISDEAAMNRAMMHDVVVRVLQMPPFIGGG"
     gene            complement(5838..6350)
                     /locus_tag="PK710_05990"
     CDS             complement(5838..6350)
                     /locus_tag="PK710_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09299.1"
                     /translation="MSSQRPSDDATRKVRRPIHRLSESLLPVFDPADDRSLAGLARFL
                     LSCRLEPAKPTTLVPSLPRNLTGGHIVRIGDGSRYVFEVTIIRDALSRCFGLYAPPPS
                     DLRTLIPELAVVDLARSRARLIHNDGREIMLSVLNVDEVTHLIVMAQAPETDAETAEF
                     EDIEIVFEDE"
     gene            complement(6581..8191)
                     /locus_tag="PK710_05995"
     CDS             complement(6581..8191)
                     /locus_tag="PK710_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09300.1"
                     /translation="MTKQHAIAALVLSVVAGIAVMAIIDKSSEDKVRPDAVTSSNIAT
                     ESVPKPEKNPGAVPVELYVMSQCPYGVEALKAIKPAIDKLGADVDVALEYIGQEDGSG
                     NLTSMHGPAEVKGNIAQLCALQHAPAKAFDLIACQNENMRQVDTNWETCASKIGVPVQ
                     PLKACIEGAEGKKLLSASFKRAEKKGATGSPTIYIGGKPYEGRRSPDAFLRAICSAHE
                     GPAPKACAELPPLATVHVTILTDKRCADCDASRLAQAVGSRIAKPDVKILDYTEEEGR
                     NLHKEIGGGSLPLLVFDDSLDGDAEANEAIGPGTQRKGKHRVLNVGGTWNPVCADPNG
                     CSQAECKNTMSCRPESKNTLEVFVMSQCPYGVLALNAMEEVLKNFGKGLHFKVHYIAN
                     GTAESGFQALHGQPEVDENIRELCAIKHYGASNKYMDYIWCRNKDIRSSEWQSCTGSN
                     GIATAKIKSCFEGEGKKLHEEDIKIANSLGIGASPTWLVNGRYKFSGIDAETIRKNVC
                     EHNPGLKGCENKLSAAGPGASPQGGCGQ"
     gene            complement(8317..9063)
                     /locus_tag="PK710_06000"
     CDS             complement(8317..9063)
                     /locus_tag="PK710_06000"
                     /inference="COORDINATES: protein motif:HMM:NF020425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HOT09301.1"
                     /translation="MSERPCEVTELHTRLLRVTLEIDACTAYWSHADPQRSHAENAKL
                     AQEQGWFGDRSPARVRKLIDELSSRFAPFPEAMSVLRNWKDMQDNTRRLICHWHVQLT
                     DTVYRKFTGDLLPELRGPETAILTRELVAEWIEQVAPSRWATSTKLKYGGNLITTAAE
                     AGLVSQPLRERTPRIPEVPDEALAYVLHLVRDVEFSGTLMDNPYLRSVGLQGDTLEDA
                     LARLAGRSEDGGFDLAWMAKNLTEWAARKS"
     gene            9587..10975
                     /gene="deoC"
                     /locus_tag="PK710_06005"
     CDS             9587..10975
                     /gene="deoC"
                     /locus_tag="PK710_06005"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOT09302.1"
                     /translation="MTSTSVALGSDHGGYTLKQAIKRHLTSKGLSVLDVGTHSTDACD
                     YPVFARAAAEAVASGRASVGIVIDGAGIGSCMTANKVPGVRAGMAFDEKTARNAREHN
                     DANLLTLGAGYLAEDLAIRIVDVFLSTECTVDRHKRRVAMIDALDGKPARSGSSMSSN
                     DYQGLVDAITRVLTANPTMISSVLGASGATGYACADCSNCGNCASKKPETVRQVLGGV
                     GGRISSSLGAKNVPTDMAKLIDHTLLKPEATYADIDRLCDEAREYGFASVCVNSMHVE
                     RCAKRLKGSGVKTCCVIGFPLGATPKEIKALEARRAIRDGAKELDMVIAIGALKSGDH
                     RYVYEDIRLVAEAARDGSALLKVIIETGLLTDEQKVAACVLAKKARADFVKTSTGFGH
                     GGATVHDVELMARAVDHQLQVKASGGVKGVDDAKKMVAAGASRIGASVGVKIVREAKG
                     LSTEGASAQGGY"
     gene            11001..11636
                     /locus_tag="PK710_06010"
     CDS             11001..11636
                     /locus_tag="PK710_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09303.1"
                     /translation="MAELRSFVFLDSLQPQFAAYLGTVCRGFLPTAGEASLFVEISPG
                     IEVNRVTDVAVKATDVRPGMQIVERLYGMLEVHSPSQADVRRAGEEILAALELQETDR
                     IKPRVLSSQIILNTDDLQVQLINRTRMGMMLVAGESLYILEVEPAAYAAYAANEAEKA
                     AEINVVDVRMTGAFGRVYLGGEQRDIDVASTAAIKAIESLSGRDVGSKRKD"
     gene            11640..12254
                     /locus_tag="PK710_06015"
     CDS             11640..12254
                     /locus_tag="PK710_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09304.1"
                     /translation="MIDLRSYVFLDSLQPQHAAFLGTVAKGFLPTRYQASLFIEVSPG
                     IAVNRLTDIAVKSTGVKPGMQIVERLYGLLEIHHDTQAEVRRAGEAILAEMGKKENDR
                     IKPKLLTSQVIRHLDDRQTQLINRVRHGHMIVAGQSLYVLECTPAANAALAANEAEKA
                     ANIHVLEITSFGSFGRLYLAGRDRDIDVAWPVAEKALELEGRAE"
     gene            12339..12656
                     /locus_tag="PK710_06020"
     CDS             12339..12656
                     /locus_tag="PK710_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOT09305.1"
                     /translation="MAEALGMIECRSFAAMVEASDAMVKAAKVELVGYEKTGGGYVTA
                     IIRGDVAAVKAAVDAGTNGASRVGEVVATHIIARPHVNVDLVLPLGRVDAVKEELAAR
                     GIK"
     gene            12824..13141
                     /locus_tag="PK710_06025"
     CDS             12824..13141
                     /locus_tag="PK710_06025"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOT09306.1"
                     /translation="MLLAKVVGTVVSTRKDPKVEGFKFLLLKQVNPETLEESGFVVAN
                     DAVGCGVGEYVLYASGSSARQTEITQDRPCDAVIMAIVDTWEIDGKVLYEKHSYEHGV
                     GDW"
     gene            13155..>14086
                     /locus_tag="PK710_06030"
     CDS             13155..>14086
                     /locus_tag="PK710_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT09307.1"
                     /translation="MTQPGISPEQIRRLAEAIAKEVSAGRSAPAVASREPVATGAEGV
                     FSTVDEAVVASRKAYEKLDALGLERRKAIIAKIRAVCLTQAEALARAAIDETALGRYE
                     DKVNKNLLVTHKTPGPEDLLPWAQSGDNGLVLVEPAPFGVIGAITPVTNPTSTIINNT
                     IAMVSAGNTVVFNTHPSAKNVSAATVRLLHQAIVDAGGPPNVVACIAKPTIESAGELM
                     HHPKVRLLVVTGGPGVVKAAMNSGKRAVCAGPGNPPVIVDPTADIEQAGKDIVFGHSF
                     DNNVICVDEKECIVVESVAEALKASMKRNGAVEVP"
BASE COUNT         2982 a         3817 c         4178 g         3109 t
ORIGIN      
        1 tctaagctct cgctgccgcg ggtaatgtcc ataaggatcc gaacgttgcg gagcgtttcg
       61 gagtggtgac gggtgttggg tacggtgccc cgatgccttc ggaagggtac aaggtgggcc
      121 cctccacgat catcaaggcg atgcccaatg cggcagcggc atggatggcg atcgtcgaag
      181 gtgttcgtgg accgaatatg tcttgcagca cggcttgtgc ttccggggcg catgccattg
      241 gcattgcttt cgatcaaatc cgtttgggcc gagcgatcgg catgttggcg ggaggggtcg
      301 acacggtggt gacgcgggat gtgatgcgaa gtttcgcgtg gttgcgcgcg gtcaatactg
      361 ccaaagacga agaaccttcg aagatgtcgc gtcctttcga caagacgagg cgcggtttcg
      421 tgttgggaga aggagcgggt tttcttgtcc ttgaggagcg tgagcatgcg atggcccgag
      481 gcgcgaccat tatcgccgag atgcgcggat ggggagcttc ttccgacgca catcatatcg
      541 tggctgtcga gcctgagggc aagggcatgg ctcgtgcgat gcgtcttgct ctatcggata
      601 gtcatatcgc tcccgaagaa atggattata tcagcgccca cggcaccagc acaccgatga
      661 atgatcgtct ggaaacgctt gccatccgcg acgttttcgg aaagcacgcg aacgatctac
      721 tcgtcagttc tcaaaaatcg atgataggtc atgccatggg aggcgcggga gccattgaag
      781 ctgtcgtcac ggtgttgacg cttgcgcatg gtgtggcgac acccaccatc aatttgcaaa
      841 ccccggatcc cgactgtgat ctcgactacg ttccccatga agcgaggaag aagcccattc
      901 gtgctgccat gagcaattcc tttggctttg gcggccatga tagcgccatc attttcgcgc
      961 aacccaacgg cctctgatcg ctctggattt tcatgccctt tgcgcatttt cggaggctcc
     1021 gatgagggcc cttgttctcg gtggtaccgg gttcgtcggc atgaacgtga cacgcgcgtt
     1081 agcctgccgt ggtcacgatg tcatcgccac gcgtcgcgct cgcgccaaca cgctttttgc
     1141 ccgcaagctc ggagccaagc tcatcgttgc cgaccttgat gatgtcgaca gcttgaccca
     1201 agccatgaaa ggtcggcaag tggtctttca ttgtgcgggg ttttatccgc ggtactcgct
     1261 gaacatcgat gaacaagtgt ccttggctca gcgaaccact cgtcatgtca tcgaagcggc
     1321 tcgtcgtgcc tgcatcgatc gattggtggt aaccagttcg gtggccacca tcggacctcc
     1381 acgccaaggt cgtgttttgt cgaatgaatc cgacgagatg tccgcggacg ctcgcgacag
     1441 tgtctacttt gcctcgaaag aagctatcga aagagtggcg ctcgaaactc gggacctcga
     1501 tgtcgttgtc acgtgtccag gaggcattgt gggtgaactg gacgtcaagg ccggaacggg
     1561 tttcatcatc gtcgccttgg cccaccgatt gctgcccttt tacgtgcaag gcaaaaccaa
     1621 tatcgtcgac gcggacgata tggcggaagg tcatctggcc gcggcggaac gcggacgaaa
     1681 agggcaacgg tacattttgg gtggccataa cacgaccatc aaggacttgt tgctgggcat
     1741 tggcgatgaa ttggggattc gtttcacatc gtggccgatg ccgttggcga ttgcgaaacc
     1801 gatggccacc ttcgatgaga tgcgttgtgc ggcgaggggc aaaggggaac gaccgttcat
     1861 ccccagggaa ttcgtggata tcgtacgttt tggacaatgg gtggataccg agcgctcgat
     1921 gtcggaactc gcgcttcgag aacctactcc gttgcatatc acgattcaaa aggcttgcgc
     1981 gtggtacgag cggtatcgat atatcccacg ccgtctggaa tcgaggagcg tgggggaaag
     2041 tccgtcgttg ccgcgcatct ttgcggcctc ctctcattcg aaatcaaccg acatatcaag
     2101 tgaatcaagg agaactccat gactgctccc agtgctgaca ttcttcgaaa cgatgtggac
     2161 gagatcattc gtcggattcg tccggacttc aaaggtgaga tcaaggtgac cgatcgtctg
     2221 cgcgaggaca ttgggctcga ttcgcttcat gcgatggagc tgctgtcggc ggtgacggaa
     2281 aagtacgaac tcgacgtaga tatcgaagaa gtgcaggagg tgaaaacagt gggggacgtc
     2341 gtgcagtttt tgacgacggc catggcgtga gtttggcgaa tcctcacggc agggaagggg
     2401 aaacatgaag attcagttca tttatccagc gttcgagcgt catgcgcaat cgcatcccga
     2461 gttgctcgac gcggttccgt gtgacgaata cttgggaggc ccttcccttg cgattgccaa
     2521 tctcgcggcg gttacgccag cgggctacga gatggcgtat tgtgatgatc gggtcacgcc
     2581 attttcgctc gatctcgacg ccgatatcta tgccctttcg ttcttcacgc ctgcggcaac
     2641 gagggctttg gagattggcg atatgattcg tggggcaggg aaaaaagtgg tggctggagg
     2701 gatttttcct tctgccctgc cggacgtggc ccagcagcat ttcgattcga tcgtgatcgg
     2761 ggaaggggaa ggcgtgtggc aacggttgct ggaagacgcc gcggccgggc gtcttgcgcc
     2821 acgatatcaa cagcaggagc cgtttgatct ggcgacgctt ccgcctcctc gcatcgattt
     2881 atatatcggt gcggaaaacg atcgtttcca tcccgacgac tatccgttgc aaacctcgag
     2941 aggttgtccg ttgcgttgtg atgcttgtgt cgtgccctgt gtcatgggca aaaaactccg
     3001 gctcgtacca caggaaacga tattacgagc ggtgcaggat cttgcctccc atggcaagcg
     3061 agctgctgtg acggaggata cgtcgttttt ctttttctcc ggcgcgcgac gacatttccg
     3121 tgagtttctc aaggctttgc gtgacgatcc gcgaccgcgg ggagagaagg tgagctacat
     3181 cggcatcacg atgcccatga ttctcgctct cgatccgaca attttggcgg atatcagcga
     3241 agcgggcatc aatcgtttct acttggtttg cggtttcgat cccatcacgc gaaaagcttt
     3301 tggcagtggc gaccaggaat ccttggacaa ggcggtggaa gccattcagc gttgtaagga
     3361 taacgatatc gagccgtata cctcgctgct tgtcggcaat gacaccgacg acgagggggt
     3421 attcgatcgt attttgtcgt tcaccgagaa ggcggatgtc cccaagacgg aatttgccat
     3481 cttcacgcca tatcctggca cgcctgcttg ggaacaaatg caagcggagg gaaggatcat
     3541 cgaccgcacg tggaagcact acaacgacgc caacgtggtg tttcgtccca agcagatgag
     3601 tcccgagcga ttgttgcagg gatatttgga tttgtggcgt gacttttata agagcaagac
     3661 ctttttggcc tcgctcgagc atggcgacag gacaattcag ttctgaggca agaggcggtt
     3721 agcgatgcca ggaaaaacca atctgtttac acccatcctc gaagcgttcc gacagatcgg
     3781 tccgcgtctt gccacaagtg gacgaacgca agaagagcaa gaggtcgttg cttctatctt
     3841 gcgagaggtg aatcggtatt gccgcaaaga agtggacggc cttgcgatcg accgacgcgg
     3901 cgcattgggg caggagatgt tggatgcggt tgccgagcga ggctggtttg gcttgacgat
     3961 tccggaagag tatggaggcg ctggcctatc catgtccgcg gccacgcgga ttgccaccga
     4021 aatctgtaaa tataacggct cgttagcgac ttgcgtcgcc ttgcattcgg gcctcgcgct
     4081 ctatggattg atccatcttg cctcgaagga tttgcaatcc aggtatctgc ccgaaattgc
     4141 cgctggacag aggattgctg ccttttgcgc taccgaaccg aacgcgggct cggatattgg
     4201 atcgatgcga acgctgctct atgagcaaga cggcatcttt cggttgcgtg gaagcaagtc
     4261 gtacgtaacc aatgggggat tcgcgggagt attgaccgtg attgcgagca gcccggggtt
     4321 gggcggtgct cgcggtggac ataccatggt cgtgatcgac ccatcgtgga cgggagtaca
     4381 taaacaggcg gaagaaaaca aactcggact caagggttct tcgaccatca cgattgattt
     4441 cgacgatgtc gaggtacctc gcgatcacgt gattggagag gtttccaaag ggttggatct
     4501 tgctcacgaa gcgttgacat ggggtcgaag cttcatggcg gcgggttgtc ttggctctgc
     4561 gatggccgcg gtacaagagg ctcgggagca tattgcgggg cgtgtgcaat ttgggcgaac
     4621 gctcgatcaa ttcccgctcg ttcgtgagca gatggctgcc gcgatggcgg atgtatacac
     4681 ggtggagacg gtattgcgcc tggtgtgcga tgttttcgat tctcgctcgg gagatatcgc
     4741 gttggactcc acggtggcaa aagtattggg ctcggaacga gcatggaacg tgattgaccg
     4801 cggccttcag ctcatgggag gagctggatt catcgaggag agggggatgc cgcgacgatt
     4861 acgagatgta cgggtcacgc gcatcttcga gggagccaat gacgtgctac gtctccattt
     4921 ggcgtctgcg acgcttggat ggccgcgggc tggtttacaa gagtttcccg aagtttctcc
     4981 gcttgttccg gcgcctcttc gaaccgaggc cgaacgtttc gatgagatgc ttcgcgaagt
     5041 tggcgtttgt ctcgcctcga tacaaaaaca atatggattc aagctgtttc aaagacagtc
     5101 actgcaatcc gtgatggccg atgcgatcat ggccacctac ggaatgatgg cggtactcgt
     5161 tcgcgctgcg gcatccgtgc aggatcaaga tacccctgag acctgtgtcg aactcgcaac
     5221 cgccaaactg gcatgcaaac gcctggaatc cgaagcccgg ttggccctgc aaaccatgac
     5281 acgaggtgct gatgacgaag ccttgacact ggcgaacgca gtgcttgccc aatgacttgc
     5341 gctactcgtc ttcccgtacc atgactcgtt cgtgtctgtc gtcgtaacca tcgaacactt
     5401 cggcctcatt cgcgaaacca tgccgtcgcc ccaggaaaag atcgtagttc ctgtgggaat
     5461 gaccgtacgc gaattgtttc aacatctgat cgaacgatac ggtgattcct atcgcgatgc
     5521 cttgttggct ccgcaaggac agccccttcc gaacgtcgta ctcacgctcg atggggaaca
     5581 gataagcgat gaagccgcga tgaatcgagc catgatgcat gatgtggtgg tgcgggtgct
     5641 gcaaatgcca ccgttcatcg gcggagggta gggcagagac acgctcgaga ttctcgcgcg
     5701 atcgtcgatg gtctgcgaca cggagagata gaccagtcgt ccgagcctcg atgacggcga
     5761 ctgcatctac gacaacgtgg ttcaaaacat caaaacgaac gtggtatccg acgagaaagg
     5821 atggaaggac cggcgcttca ctcgtcctcg aacacgatct cgatgtcttc gaactcggcc
     5881 gtttcggcat cggtttcagg agcctgcgcc atcacgatga gatgggtgac ttcgtcgacg
     5941 ttcaatacgg acagcatgat ttcgcgtccg tcgttgtgga tgagtcgcgc tcgtgagcga
     6001 gccaagtcga ccacggcgag ttctggaatc agggtacgga ggtcggaagg tggtggagca
     6061 taaagaccga agcatcgact gagggcatca cgaatgatcg tgacttcgaa tacatagcgg
     6121 cttccatcgc cgatgcgtac gatgtgtcct ccggtcagat tgcgaggaag gcttggcacg
     6181 agggtggttg gtttcgctgg ttccaggcgg caactcagca aaaaccgtgc gagtcctgct
     6241 aacgatcgat cgtcggcagg atcaaaaacg ggcaagagcg attcactgag gcgatggata
     6301 ggccgacgaa ccttacgggt ggcgtcatcg gaagggcgtt gcgaactcat gattgactcg
     6361 tgggcatggg cgatcccgcg cccggggcga atcatagtca tatttggcgt gttcgcggaa
     6421 caccttgtga tgggctttga tctttcgagt catcattggc gtggtgggca acgatcgaaa
     6481 gatgggcgat cgaccgaaac ggggtgaaag gaagaaattg gcaaagaggg taagaaaatc
     6541 gttgatctat ggattatgtc ctatcgggta gcgttcgttt tcattgtccg cagccgcctt
     6601 gaggacttgc gccggggcct gcggcggaca gcttgttttc gcatcctttc agtccaggat
     6661 tgtgctcgca gacgttctta cggatcgttt ctgcatcgat tcccgagaac ttgtatcgac
     6721 cattgacgag ccacgtggga gaggcaccga taccgaggga attggcgatt ttgatgtctt
     6781 cttcgtggag ttttttccct tcgccttcga aacatgactt gattttggcc gttgcgatac
     6841 cgttcgaccc cgtgcaggac tgccactcgc tcgatcgaat gtctttgttg cggcaccaga
     6901 tgtagtccat gtacttgttc gaagcgccgt aatgcttgat ggcacaaagc tcgcgaatgt
     6961 tctcatcgac ttccggttgc ccatgcaagg cttggaaccc tgattccgcg gtgccgttgg
     7021 cgatataatg cactttgaag tgcaaacctt ttccgaaatt cttgagcact tcctccatcg
     7081 cgttgagcgc caacaccccg tagggacatt gtgacatcac aaatacctcc aaggtgtttt
     7141 tggattcggg acggcaactc atcgtgtttt tgcattctgc ttgagagcaa ccattgggat
     7201 cggcacatac ggggttccat gttcccccca cgttgagcac gcgatgctta cccttgcgtt
     7261 gggtacccgg tccgatggct tcattggctt cggcatcgcc atcgagactg tcgtcgaaca
     7321 cgagcagggg aagcgatcct cccccgattt ccttgtggag attgcgccct tcttcctcgg
     7381 tgtagtcgag aatttttacg tcaggttttg caatacgtga tccaactgct tgcgccaacc
     7441 gggaagcgtc acaatccgcg caacgcttgt cggttagaat cgtcacgtgg accgtggcga
     7501 gcggaggaag ctcggcacag gccttgggcg cggggccttc atgagcgctg cagatggcgc
     7561 gaaggaatgc gtcgggacta cgccgtccct cgtagggctt gccaccgata tagatggtgg
     7621 gacttccggt ggcacctttt ttctcagccc gcttgaacga agcggagaga agtttcttac
     7681 cttccgcacc ttcgatacaa gctttcagcg gttggaccgg caccccaatc ttgctcgcgc
     7741 aggtttccca attggtatcc acctgacgca tattttcatt ctgacaggcg atgaggtcga
     7801 acgctttggc gggggcatgc tgcaatgcgc atagctgcgc aatatttcct tttacttccg
     7861 ccggtccgtg catcgacgtc aggttcccgg aaccgtcctc ttgaccgatg tattcgagag
     7921 ctacatccac atcggctccc aacttgtcga tggcgggctt gatggccttg agagcctcca
     7981 cgccataggg gcactgggac atcacataca attcaacagg aaccgccccc gggttttttt
     8041 ccggtttggg aaccgattcc gtggcgatat tcgatgacgt tacagcgtca ggacggactt
     8101 tgtcttccga gcttttgtcg atgatggcca tgacggcaat gcctgccacc acggaaagaa
     8161 cgagcgcggc gatcgcatgt tgtttggtca ttggtgatgc ctttgggcga atgaatgagt
     8221 gtgaaaagcg atgctttgaa tgtggaaata ggttggaaac ctaatcacgt ctcgtgggtc
     8281 gaggcgaggg aaatatgccg acgaaagcgt tgtctctcac gactttcgag cggcccattc
     8341 ggtcaaattt tttgccatcc aggccaagtc gaatccgcca tcttcgcttc gtccagccaa
     8401 acgagcgaga gcgtcttcga gcgtgtctcc ttgaagaccg acggagcgca agtacgggtt
     8461 gtccatcagc gtgccggaaa actccacatc gcgaacgagg tgcaatacgt aggctagcgc
     8521 ttcatcgggc acttccggaa tccgtggtgt tctttcccgc aacggttgag agacgagtcc
     8581 cgcctcggcc gcggtggtga tgaggttgcc accatacttg agtttcgtgg aggtggccca
     8641 tcgcgaaggc gcgacttgtt cgatccattc cgccaccaat tctcgcgtca aaatcgccgt
     8701 ttccggtccg cgtagctcag gaagaagatc tcccgtgaac ttgcgataca ccgtgtccgt
     8761 gagctgcaca tgccaatgac agatgagtcg tcgcgtgttg tcctgcatgt ccttccagtt
     8821 tcgcaacacg ctcatggctt ccggaaaggg agcgaaccgc gaagacaact catcgatcag
     8881 cttgcgcacg cgggcagggg aacgatcgcc aaaccatccc tgctcctgag caagtttcgc
     8941 gttttccgcg tgggaacgtt ggggatcggc gtgagaccaa taggccgtac aagcatcgat
     9001 ctcgagagtg acccgcaaca atcgggtatg taactcggtg acttcacaag ggcgttcgct
     9061 catgggtctt tcgctattct ggtctgcgcc tcgtgcaacg gactggttgg gtttcgggtt
     9121 cataccttgc gcgagaacga ctaccccgcg cacgagccgt tgtcgaccat cttatgcgtt
     9181 ctcatgcaaa gaccttcatc aaacggcatt tttcttcacc atccgcagcc catgccgcgt
     9241 gccgtgggct ccagccggtg cgcgatgggc gctggacgga cctcaaacca tccatttcat
     9301 gaatgcctgt accatgccct ggggcttgcc ccatcagcgg tcagcagctt tcaatgactt
     9361 tccgagaaac gtgtaagtat tgcaaaatac aaactaaata cccggttcgc gcattgcgag
     9421 ttctggggcc gtggtttgaa atccgcctat ggatatcctc cgggctgccc accaccatga
     9481 gcctttggcg catcctgggc gcgatgggat gcgtgcttga cgtccgggcg tcggcaacct
     9541 accgtgtccc gctcgcgtct gtgatcgttg gaggctttca tacgacatga cttcgacttc
     9601 agtggccctg ggcagtgatc atggcggcta cactctcaaa caagccatca aacgtcacct
     9661 aacgtccaag ggcttgtcgg tgctcgatgt cggtactcat tcgacggacg cttgtgatta
     9721 tcccgtgttc gcacgggcgg cggcggaggc tgtggcatcg gggcgcgctt ccgtaggcat
     9781 cgtgatcgat ggcgccggta ttggctcatg catgactgcc aacaaggtgc cgggcgttcg
     9841 agcgggcatg gctttcgatg aaaaaaccgc tcgaaatgca cgggaacaca acgatgccaa
     9901 cctcttgacc ttgggggctg gctacctcgc tgaagacctc gcgattcgga tcgtcgatgt
     9961 ttttctttcc acggaatgca cggtggaccg tcataagcgc cgcgttgcca tgatcgatgc
    10021 gctggacggc aaaccggcca ggagcggatc gtccatgtca tcgaatgact accaagggtt
    10081 ggtggatgcg atcacgcgcg tgcttaccgc gaatccgacg atgatatcgt cggtgctggg
    10141 agcatctggc gcgacaggct acgcgtgtgc ggattgctcg aattgcggca attgcgcttc
    10201 gaaaaaacca gaaacggtca gacaggtgct cggaggcgtc ggcggacgca tctcctcgtc
    10261 gttgggagcc aaaaacgttc ccacggacat ggcaaaactc atcgatcaca ccttgctcaa
    10321 gcccgaggca acctatgctg acatcgatcg cctttgtgat gaagcccggg agtatggctt
    10381 tgcttccgtg tgtgtgaact cgatgcatgt ggagcgttgc gcaaagcgtt tgaagggcag
    10441 cggcgtgaaa acctgttgtg tcattggttt tcctttgggg gccacaccca aggagatcaa
    10501 ggccttggag gcgcgacggg ccattcgaga tggcgcgaaa gagctggaca tggtgatcgc
    10561 gatcggcgca ctcaagtctg gcgatcatcg ttacgtatac gaggatattc gactggttgc
    10621 ggaagccgcg cgcgatggtt ccgcgcttct caaggtcatc atcgaaacgg gtctactcac
    10681 cgacgagcaa aaggtagcgg cttgcgtact tgccaaaaaa gcgcgtgcgg acttcgtcaa
    10741 aacttccacc ggcttcggtc atgggggcgc taccgtccac gatgtggaat tgatggcacg
    10801 ggccgtagac catcaactac aagtcaaagc atcgggcggc gtcaaagggg tagacgacgc
    10861 caagaaaatg gtggctgcgg gcgcttcgcg tatcggtgca tccgtgggcg tgaagatcgt
    10921 tcgggaagcc aagggtcttt ccacggaagg agcctccgcc caaggcggtt actaggccac
    10981 tggcccaagg agccacgatc atggctgagc ttcgaagctt cgtgtttctc gactcgttgc
    11041 agccccaatt cgctgcctat ctcggcaccg tgtgccgcgg attcttgccc accgccggag
    11101 aagcctcgct tttcgtagaa atctctccag gcatcgaagt caatcgcgtc accgatgtcg
    11161 ccgtcaaagc caccgatgtc cgacccggaa tgcaaatcgt cgaacgcttg tacggcatgc
    11221 tcgaggtcca tagcccttcg caggcggacg tccgacgtgc cggcgaggaa atccttgccg
    11281 ctctggaact gcaagaaacc gatcggatca agcctcgcgt gttgtcttcc cagatcattc
    11341 tcaacaccga tgacttgcag gtgcaactca tcaaccgcac gaggatgggg atgatgctcg
    11401 tggcgggtga atcgctctat atcttggaag tagagcccgc ggcctatgcc gcctacgccg
    11461 ccaacgaagc cgaaaaagca gcggaaatca acgtggtgga cgtgaggatg accggtgcct
    11521 tcggccgcgt ttatctcggc ggtgagcaac gggatatcga tgtggcttcc accgcggcga
    11581 tcaaggccat cgaatcgtta tctggccgtg acgtgggaag caaaaggaag gactgagcca
    11641 tgattgacct gcgaagctac gtgtttctgg actcgttgca gccgcagcac gccgcttttc
    11701 tcggcaccgt ggcgaagggc ttccttccca cgcggtatca agcctcgctg ttcatcgagg
    11761 tgtctccagg catcgccgtg aaccggctga ccgatatcgc cgtgaaaagc acaggcgtga
    11821 aacccggcat gcaaatcgtc gagcgcctct atggcttgct tgaaattcat cacgataccc
    11881 aggctgaagt gcgaagagct ggggaagcga tccttgccga aatgggcaag aaagaaaacg
    11941 atcggatcaa gcctaagtta ttgacttcac aggtgattcg tcatctcgac gatcgccaaa
    12001 cccagctcat caaccgggtc cgtcatggtc atatgatcgt ggcgggacaa tccctttatg
    12061 tgctcgaatg cactccggcc gccaatgcgg ccctggccgc caacgaagcc gaaaaggccg
    12121 ccaatatcca cgtgctggaa atcaccagct ttggatcttt tggacgtttg tatctggctg
    12181 ggcgcgatcg tgatatcgat gtcgcctggc cagtggcaga aaaggccctc gaactcgagg
    12241 gacgcgcaga gtgaaacgca agttggaata cgccctcgat ggcgtgtgga tgatgcaacg
    12301 atagggacgg gcaaccgacc ctgctacgga ggaaagaaat ggctgaagca cttggcatga
    12361 tcgaatgccg ttcgtttgcc gcgatggtcg aagcttccga tgcaatggtg aaggcggcca
    12421 aagtcgaact cgtcggttat gaaaaaaccg gtggtggcta tgtcaccgcc atcattcggg
    12481 gagatgtcgc cgcggtgaaa gccgccgtcg acgccggcac caacggtgcc tcccgggttg
    12541 gtgaagtcgt ggctacccac atcatcgctc gcccccacgt caacgtcgac ttggtgctgc
    12601 ccctcggtcg cgtcgatgcg gtcaaggagg aattggccgc gcgcggcatc aagtagcaac
    12661 gatggcccat cttccgctta tgctgcgatg ggcatgagag aacgaaaacg tctggcgagg
    12721 gggcctcgtg cgcggcctgg caaggctcgc tcgccaacgt tgccattggc tgattcgacc
    12781 ggagggacaa cccccggagc gctgggccgc tcgaggtgat gtgatgctcc tggcaaaggt
    12841 ggtcggtacc gtggtgtcca ctcgaaaaga ccccaaagtc gagggcttca agttcctcct
    12901 gctcaagcag gtgaacccgg agacgctcga ggaaagtggt ttcgtcgtgg cgaacgacgc
    12961 cgtcggatgt ggcgtgggcg aatatgtctt atacgcttcc ggctcctccg cacgacaaac
    13021 cgaaataacc caagaccgcc cctgcgatgc cgtcatcatg gccatcgtgg atacatggga
    13081 aatcgacgga aaagtgctct atgaaaagca ttcttacgag cacggggtcg gagattggtg
    13141 agaaaggttg agccatgaca caaccaggga tcagcccaga acagatccgt cgcctggctg
    13201 aagccatcgc caaagaggtt tcggcaggac gatccgcacc cgccgtcgcc tcccgcgaac
    13261 ctgtcgccac cggcgccgaa ggtgtttttt ccaccgtcga cgaggccgtg gtggcttctc
    13321 gcaaagccta cgaaaaactt gatgccctcg gcctcgaacg ccgaaaagcc atcattgcca
    13381 agatccgcgc ggtctgcctg acgcaagccg aggctctcgc acgcgctgcc atcgacgaaa
    13441 ccgccctggg ccgctacgaa gataaagtca ataaaaatct gctagttacg cataaaaccc
    13501 cgggccccga agacttgctc ccctgggccc aatccggcga caacggcctg gtgctcgtcg
    13561 aacccgcccc ctttggtgtc atcggcgcga tcacccccgt caccaatccc acctcgacca
    13621 tcatcaacaa caccatcgcc atggtttccg ccggcaacac ggtggtgttc aatacgcatc
    13681 ccagcgccaa aaacgtctcc gccgcaaccg tgcgattgtt gcaccaggcg atcgtcgatg
    13741 ccgggggacc acctaacgtt gtggcatgca ttgccaaacc caccatcgaa tccgccggcg
    13801 aattgatgca tcaccccaag gtccgcttgc tcgtggtcac tggcggtccc ggtgttgtca
    13861 aagccgccat gaactcagga aaacgcgcgg tttgcgccgg ccccggcaac ccacccgtca
    13921 tcgtcgaccc caccgccgat atcgaacaag ccggaaaaga catcgttttc ggccattcct
    13981 tcgacaacaa cgtgatctgt gtcgacgaaa aagaatgcat tgtcgttgaa tccgtggccg
    14041 aagccctgaa agcttccatg aaacgcaacg gtgcggtcga agtccc
//
LOCUS       DAOSOR010000075        13978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4392_length_13978_cov_4.509445, whole genome shotgun sequence.
ACCESSION   DAOSOR010000075 DAOSOR010000000
VERSION     DAOSOR010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 13978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13978
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4392_length_13978_cov_4.509445"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            400..1371
                     /locus_tag="PK710_06035"
     CDS             400..1371
                     /locus_tag="PK710_06035"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT09308.1"
                     /translation="MQHHSKAIDMQLRNSTTESDRNLKDALPHPPLCDTTKLMRAHPW
                     LAVVVFWACSPAVPKQPMKSSTMTGVNTATVASAPVPAQTEPGPSPQPTQPTSPPVQD
                     TSCPSGMQWIAGGSFLMGSEQGDGDEKPVHRVELDGFCLDETPVTVGEYARCSGCTKP
                     NTGKDCNWGILGRGNHPVNCVDWNQATAYCRLVGKQLPTEAQWEYAARGGARQLEYPW
                     GSEQPQGRACWSRTEGTCAVGTYPSGAFTLKDMAGNVWEWLQDWYGPYPRSATKNHVH
                     ESYGSSRVCRGGSWNYHFPSGLRGSDRIFVTPVSRYNSLGFRCAKAK"
     gene            1537..3732
                     /locus_tag="PK710_06040"
     CDS             1537..3732
                     /locus_tag="PK710_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT09309.1"
                     /translation="MHIRIHRSKTPSGKLRQYAQLVHSYRRPDGMPAHRVLATLGDPD
                     SLLVQNMRKALADARMGKRSDSDPPRSALPTRPVRNLRYLDAAVLLELWKRLGLDALL
                     EKLMPQGQADLAPSNLVACLVIQRCLEPDSKLFACRWVPNTALPQLLGFDPDSFHNTH
                     IHRVLEQLDEATPSLMAQLPKLIQQHSGAFRCLFLGVSDTWFVGQDPSYAQYAKTKEG
                     MSKRKIGIVLLCNEDHLPLHWQVVEGKYCEAVAMTDMLRAVSALSWADALPVVMDRAV
                     EQSTRLQQLQALDVRFVTALCKAEFNSYATQVPYDCTRALQVDDSNRQQIESMAAQCV
                     EQAGLTRVDDDLFVLDLGIVQREEIDQVATTMVQESDTLLHAMRVCQQLQQTGVDQRY
                     PSQAACCRALGINKSLGSLYKRLHTLLPDIQQDILAGRAEGYSFESLCQIAKLPNDKQ
                     RDAFDALLARAPARKKTKRTQLVPKQPDTPGKGRAVAYFNPQLFVDKRVRAHRKRERV
                     HSKIQDIVRKANDNPNRYTVGSLRLQVDRILNKESLLDVYSVDIRVTRDSGSNRFDIS
                     VQLDPTKWEAKRRYDGWNILVAHPMLQDKSASELCKLYRAKDAIEKDFQVIKSFTELR
                     PMRHRTDDKVRAHVTICMLALLLERTLHKLLSGKVKRKAKSPESLSAVAALELLEPCR
                     LNCYAPTDDGPPAYLLTATQSEQQRILARLKMTHLSQDESLAELLEKGS"
     gene            3996..4433
                     /locus_tag="PK710_06045"
     CDS             3996..4433
                     /locus_tag="PK710_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09310.1"
                     /translation="MTTIFSAETRILICQHCWAPLESTLAGGNIACRFCGTQNQVSVR
                     DDRPMFNVDYQAPQNETERIERLRSQDGRPFVPPSGLQDLVPHGQIVPWKVQEVVVVW
                     QQARHEVATNGSFDAAEQLLFLTVALSNYFGDQRDEVRERAMV"
     gene            4593..5282
                     /locus_tag="PK710_06050"
     CDS             4593..5282
                     /locus_tag="PK710_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09311.1"
                     /translation="MSRALIDTAKQNWNAVLRVLGTNDSDIPIMDSFDTLAAVLRANA
                     LQRTGQEQEATALLRKALSTLGAVARPVLNRLLQTYAPLGLCAQSYPSAVQQRSQAAA
                     ENANAIDVKKFLFFLVSALGCGGTGVFVFVMSIVGIIEPGGMVAGVVFVIVGLIHLAI
                     MYHDLNRRMKDKYIWLHGIQATERVVEIKNRRGPINNVVTMMFEAMVQVEGQSDYKAS
                     LSMTLNEKKPP"
     gene            complement(5510..10261)
                     /locus_tag="PK710_06055"
     CDS             complement(5510..10261)
                     /locus_tag="PK710_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT09312.1"
                     /translation="MIKQGTVLQHRYEILHPLGKGASSSALLARDLLRNDLVTLKLLH
                     SHDPALTAALRQEFAILVGRFHPHLPLVRDLEKQADHLFYTADFIDGTPLTPWAQSRS
                     AHDILTPLAHILDALHFLHRLGIRHGDIKPDNILVTPQGQGVLIDLGCARPLKATLDT
                     ISGTLGFMAPELLEGGPVDIRADLFAFGITCSIVLPTPAPELSNLLQRLIDPNPDHRP
                     SDAAEVNEAFGFPPFTLGPPQGRLERIVGRQAPLKSFQEALSALRQRAPHPRCLVFHG
                     PDGVGRSRLLNEMKWQAQLEGAVIQGDARAPRAIEDMFARALGSSAIDRQGLSAIVQT
                     RHELAQRSQPVVLVLDDIHLLASDQRELLDAWLRLLEPTDPWLLLLTSRHEPTLKNQE
                     ITTYSLGPLDASGVLAWTQGLLSPAVVPHVLRLTGGYPADLEALMDRVLRGEIPESDL
                     PHASAPQSAGGLFLSLPKEEKLVLGWLAVAMCDDATLRSCCQTTSHVLLRLHQRGWIE
                     REGRHWKLQRGWIAPRILASIDRETVTSIHRWHAEKSADNPPMRAYHFACGAESFQAG
                     QLLLEYASLIEQSPQAWRPVTDALEEAVSGGERVPPEVQVACAQVNRLCGRFEQALRW
                     IARCLRSRPGVSVRIACRMEAALCFLASGRLQRAETAINTALFLAEKPDVKACLLETR
                     CRIAIQQGNYEHVLTNGKIGLHLTSEASTRAKLHASMGLACTYLGQMEQAVEHLEKAN
                     TANPDALTPREKVRLSSFSAILAYRQGDLPRAIDGYRQASRWAEEHGLGDQMVSTAMN
                     LGTAQQQAGEWGNALASYERGLLIARAIGKGNTACTLTLNLANLYAGIGVYDRARTLL
                     STLRNTRDLGYVEANRLALEAELFEAEGLIDKAMTGYERAERLFSQQGAQREVAEMCW
                     HQASLLLQQGNHAQADSLRQSLFQTLSDISAPDLTARWHALSARSLLGRHPAQALSHA
                     QQALISAAASKQSALDAEMQTLMADIYEQQGASQLACDHRLQARRAWESMAVGLPESF
                     RPAFWAHPRRSAHRPEPSLKAAPPTPRELLRILEINRRLNSSLATHDVLSFALDAAIE
                     LSGAERGFLLVRSKPNSKQLDVAVARNLDKERIGRSHLKFSRSIAENVVKTGLPVTTT
                     DALTDDRFRSHQSVHAMRLRSVVCVPVTTPQGVLGAIYLDHRFRKDQFDEDMLHLLGA
                     FADQVAIALRNARLVEDLERRTEALQKEQERVQQLLEGQAATIEKLSSEVQTRQQALE
                     HRYDYSSIIGTSAPMQAVFHTLDRVTDMNIDVLVQGESGTGKELVARALHFNGPRRQG
                     PFLSINCAALPESLLESELFGVVKGAFTGAHTDRKGLLVSACGGTLFLDEIGEMPLGM
                     QAKLLRVLQERRVRPLGSSQEIPIDIRLVSATNRHLLDEISAGRFREDLYYRIAVVEV
                     RLPPLRERPEDIPALCHHILHQAASSLGKQAATLRPEALRALLRHPLPGNVRQLDNLL
                     RRAWVLAEGNAIGLADLDLFPAMSVDSKSKPRDRKAFEQEERAKILAHLESKRWNISE
                     VARAIAMPRNTLYRKLLKYGLHRPQNGAQSE"
     gene            10511..10879
                     /locus_tag="PK710_06060"
     CDS             10511..10879
                     /locus_tag="PK710_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09313.1"
                     /translation="MKTTNLFLSLALALPLYACGSDASDEDINGSWDKLTPRVCEYDG
                     KSYKVGDSFPAADGCNSCACNEDGSVVCTLVDCERVTCEYNGKSYEVGDSFPAADGCN
                     SCACNEDGSVVCTLVDCELS"
     gene            11447..12019
                     /locus_tag="PK710_06065"
     CDS             11447..12019
                     /locus_tag="PK710_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09314.1"
                     /translation="MAHRLSPVLLVLVTLLACVHCGINPVPEPPSGEPIAPPAIDNIT
                     VYPFQIMTEGNISIHAGPNTAEPGSTLWAMNIDGTGPAVTTKVHDDGSFALEITAGSG
                     DEVRLQVRSGQQRSKPIDVIIPSEEGPPTLAVRPLAGCLSLEPAFELELPPPAGSQPP
                     SASILIKNRCSSPISTFASLPRLSPCCLRN"
     gene            12001..12312
                     /locus_tag="PK710_06070"
     CDS             12001..12312
                     /locus_tag="PK710_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09315.1"
                     /translation="MLPSELTIAPGNTAEVVVQVFGDAPREEILLLETQAPELDRRPI
                     TLFGQGGIHGLLPNCSLVGGRCLSDPMDVTFPANCEALGMKYLEATCQAMNLSCCGDA
                     P"
     gene            12344..13075
                     /locus_tag="PK710_06075"
     CDS             12344..13075
                     /locus_tag="PK710_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09316.1"
                     /translation="MKLATNWMVLALSFPLFACSGTVSNEGTHSASEKLSRQEAKALD
                     KADWPTDYCEVNGWYGDGVYCDDFCPLPDPDCAQSCASLGGECLSDPMDVGFGANCEQ
                     LGRRTLTGACEAFNQTCCSAPSSSNPTCEEMGGTCLSDPMDVGFGAKCEQLGRVTMSG
                     RCEAINQTCCSDKKERTPVEEHCIRNAGDSCEADADCQTGGCGGELCYNPAFEGGIST
                     CDCAEPQPPLVAGCGCVDGVCSWYK"
     gene            13422..13682
                     /locus_tag="PK710_06080"
     CDS             13422..13682
                     /locus_tag="PK710_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09317.1"
                     /translation="MTKDSETMSDDKIEHLMAQWGFSNVKKPILPDERILAKVFEHFR
                     ELSKHPEIAAIVKRQSDGEITRKQMWDDMRAVAKEIDKANKK"
BASE COUNT         3181 a         3859 c         3909 g         3029 t
ORIGIN      
        1 aaaagtttcc cgatttcctt tgggcccaag caatgagctg aacccaatgc cttgaacgac
       61 caagccccca tttgctatta gaaccgccga tcaccaagat cctcgatagc ctcggcatgc
      121 ttcgagccca ccgttgcctc atgcgtgcgc tcgggaccca caactggcct tcgacctgct
      181 cctgtccgtg gcgtagctgc tggccgcaaa acggcagtgg gttgtacgct gcgggtcacg
      241 gcggcaacga atcgatctct ctcgaggtcc ccttcgctcc acggatgact gctggtttcg
      301 tgctccgtgc tcgatcgcgc ggacagcgta ccgcgttgag gttcgactct gacttgcgaa
      361 aagctggggt agacacacgc gatacacgga aaatcgtgca tgcaacatca ctccaaagcc
      421 attgacatgc aactgcgaaa ctcaacgacc gaatcggacc gaaacctcaa agacgccttg
      481 cctcatcccc ccctttgcga taccacaaag ctcatgaggg ctcatccctg gcttgcggtt
      541 gtggttttct gggcttgcag ccccgccgtg ccaaaacagc ccatgaaaag cagcacaatg
      601 acgggcgtaa acaccgctac ggtagcgtct gcgcccgtac ccgcgcaaac tgaaccggga
      661 ccatcaccac aaccgacaca gccgacgtcc ccacccgtgc aagacacgag ctgtccaagt
      721 gggatgcaat ggattgcggg aggttctttc ttgatggggt cagagcaggg cgatggtgat
      781 gaaaaacccg tacatcgggt ggaactcgac gggttttgtc tcgatgaaac accggtaacc
      841 gtgggagaat acgcacgttg cagcggctgc acgaagccaa acaccggcaa agactgcaac
      901 tgggggatcc ttgggcgagg gaaccatccg gtcaattgcg tggattggaa tcaagcaacg
      961 gcgtattgcc gattggtggg taagcaattg ccgacggaag cgcagtggga gtatgccgct
     1021 cggggtggtg caaggcagct cgagtatcca tgggggtccg agcagccgca ggggcgtgct
     1081 tgctggagcc gcacggaggg aacgtgtgcc gtagggacct atccctcggg agcgtttacc
     1141 ttgaaggata tggccggaaa cgtgtgggaa tggctacaag actggtatgg cccgtatcca
     1201 cgatctgcca ccaaaaacca tgtccatgaa tcctacggct cgagccgtgt gtgccgcggc
     1261 ggcagttgga actaccattt tccatcggga ttacgcggat cagaccgaat cttcgtcacg
     1321 cccgtcagcc gctacaatag tctcggattc agatgcgcca aggccaagta gcccattttg
     1381 atcgtcacag tgcgtcaatg cctctatcaa tagccattgt ttgtcatctt gcagttacta
     1441 atagatcttt ctcacgggaa atatatgcgt cattctattc tttgaattat gttttttctc
     1501 ttttcatcca gcgaaatagt agatactcta taaaacatgc acatccgaat ccaccgcagc
     1561 aaaaccccca gcggtaagct gcgccaatac gcccagcttg tccatagcta ccgacgcccc
     1621 gatggcatgc cggctcatcg cgtcctcgcc acgctggggg acccggactc gctgctcgtc
     1681 caaaacatgc gcaaggctct ggccgatgct cgaatgggaa aacgatccga ttcagatccg
     1741 ccgcgctccg ctttgccaac acgacccgta cgcaacctgc gctacctcga tgcggccgtc
     1801 ttgctcgagc tttggaaacg actcgggctc gacgctttgc tcgaaaaact tatgcctcag
     1861 gggcaggccg acctggcccc cagcaacctt gtcgcctgcc tggtgattca gcgctgctta
     1921 gagcccgact ccaaactttt cgcttgccgc tgggttccca ataccgccct gccacaactt
     1981 ttgggctttg acccagactc gttccacaac actcatatcc acagggtttt ggagcaactc
     2041 gacgaagcca ctccctcgct gatggcccaa ttacccaagc tgattcaaca gcactccggc
     2101 gcctttagat gcctttttct cggcgtttcc gacacgtggt ttgtcggcca ggatccttcc
     2161 tatgcccagt atgccaagac caaggaggga atgagcaagc gcaagatcgg catcgtgctt
     2221 ttgtgcaacg aggaccactt gccccttcac tggcaagtgg tcgaaggcaa gtactgtgag
     2281 gctgtagcca tgacagatat gctgcgcgcg gtctccgcac tgagctgggc cgatgcgctg
     2341 ccggttgtca tggatagagc cgtggaacaa tcgacgcgct tgcagcaact gcaggctctc
     2401 gacgtacgct ttgtgaccgc cttgtgcaaa gctgaattca attcctatgc gacccaggtt
     2461 ccctacgact gcacccgtgc gttgcaggtc gacgactcca accgccaaca gatcgaatcg
     2521 atggctgcgc agtgtgtaga gcaagcagga ttgacccgcg ttgacgacga cctttttgtt
     2581 ttggacttgg gcatcgtgca gcgagaggag attgaccaag tcgcaacgac aatggtgcaa
     2641 gaatcagata cgctgcttca tgcgatgcga gtctgccagc aactgcagca aaccggggtg
     2701 gaccaacgat atccttcgca ggccgcctgt tgccgagccc ttgggataaa caaaagcttg
     2761 ggaagtctgt acaagcgtct gcacacgctg ctcccggaca ttcagcagga tattctggcg
     2821 ggcagagccg agggctatag cttcgagtcg ttgtgccaga tagccaaact acccaacgac
     2881 aagcaacggg atgccttcga tgcgctgttg gcacgggctc cagcccgtaa aaaaaccaaa
     2941 cgcacccagc tcgtgcccaa acaaccggac acgccaggaa aagggcgagc tgtcgcttac
     3001 ttcaatcccc aactgttcgt ggacaagcgt gttcgcgccc atcgaaagcg cgagcgcgtg
     3061 catagcaaaa tccaagacat cgtgcgcaaa gccaacgaca accccaaccg atacacggtg
     3121 ggtagcctgc gattgcaggt ggaccggatt ttgaacaagg agtcactact cgacgtatac
     3181 tcggtcgaca tacgcgtcac gcgggactcg ggctcgaacc gattcgacat atccgtgcaa
     3241 ctggacccca ccaaatggga agcaaagcgt cggtacgatg gctggaacat ccttgttgcg
     3301 catccgatgc tccaggacaa gtccgccagt gaactgtgca agctttatcg cgcgaaagac
     3361 gcaatcgaaa aagactttca agtgatcaaa agcttcaccg aactgcgccc tatgcgccac
     3421 cgcaccgacg acaaggtgcg tgcgcacgtc acgatctgca tgctggccct gctgctggaa
     3481 aggacactac acaagctttt atccggtaag gtaaaacgca aagccaagtc acccgaatcg
     3541 ttgtcggctg tcgctgccct cgagttactg gagccttgtc gactcaactg ttatgcgccc
     3601 accgatgacg ggccaccggc gtacctgctc accgctacgc aaagcgagca gcagcgcatc
     3661 ctggccaggt tgaaaatgac gcacctttcg caggacgaat ccctcgccga gcttctggag
     3721 aagggttcat agcccacgag tatcgatgcg ttacgcttaa gtatcggtat ttaatcatat
     3781 ttaatataag gtgatcaaaa tggggtcaag ggcaaagtca aagttcaggg gtcatgctgc
     3841 cgagtcatct cggttcaaaa aactcgtcct attctcttgc gttttttcat cccagcgtac
     3901 gccccatggt gtagactcaa gaaccgtcta ctatcctgat tgaaacagat gggagataaa
     3961 atggtttcgt aagccagatc gtccgtgagg gagccatgac aacgatcttc agcgcagaga
     4021 cgcggatatt gatttgccaa cactgttggg cccctctgga gagcaccttg gctgggggca
     4081 acatcgcctg tcgtttctgc ggtacacaaa atcaggtctc cgttcgtgac gaccgcccga
     4141 tgttcaacgt ggactatcag gctccgcaaa acgagaccga acgaatcgag cggttgcgtt
     4201 cgcaagatgg acggccattt gttcccccat cggggcttca ggacttggtt ccccatgggc
     4261 aaatcgttcc gtggaaggtg caagaggtgg ttgtggtctg gcagcaagcg cgccatgaag
     4321 tcgctaccaa cggctcgttt gatgcggccg agcaattgct gttcttgaca gtggcgctct
     4381 ccaattattt tggcgatcag cgtgatgaag tccgcgaacg agccatggta tagagcgccc
     4441 tcgatgtggt acggctccca cggcacaagc agatactgcg ggggtttctc gcacgctccg
     4501 cggtgcgctc cggcgacctg caagctgcgg aaacgtggct ggcgccgtgt gacccacgct
     4561 ccgatgacct tgagaccgat accgcctatc ggatgtcacg cgcgctgatc gatacagcca
     4621 agcagaattg gaatgctgtg ctccgtgtct taggcacgaa tgactcagat atccctatca
     4681 tggattcgtt tgacaccttg gcggcggtgt tgcgagccaa cgcgctgcag aggacaggac
     4741 aggagcaaga ggcgacggcg ctgctacgaa aggcgttgtc aacgctaggg gccgtggcac
     4801 gaccggtcct caatcgactt ctacaaacct acgcccccct cgggctttgt gcgcaaagtt
     4861 acccgagcgc tgtgcaacag cgtagccaag ccgctgcgga aaatgcaaat gccatagatg
     4921 taaaaaagtt cttatttttc cttgtctctg ctctggggtg cggtggtacg ggcgtcttcg
     4981 tgtttgtgat gtcaattgta ggaataatag aacctggggg aatggtcgca ggcgttgtct
     5041 tcgtgattgt tgggctcatt catttggcga tcatgtacca tgatctcaac cggcgcatga
     5101 aagataagta tatttggctc cacggcatcc aggctacaga gcgagttgtc gagattaaaa
     5161 atcgaagggg accgataaac aacgttgtga ccatgatgtt tgaggccatg gtgcaagtgg
     5221 aaggacaatc tgattacaag gcgagcctgt ctatgactct taatgaaaaa aagccgccct
     5281 gattgtaccc aatgcgatta tctctgttcg tgtagacccg acggacccag cttatatctt
     5341 ggttgaggca aattaaagac ataattgcta tttcccagaa aagccatgtt tcgatacttt
     5401 tctgagctgt gccctgctcc ttgtgtctgc catggtgagg gaacggtgga atcgataaga
     5461 agaaaaacct gatatgaacc tttcgttccc gtgcgcggac aaagatcaat cattccgatt
     5521 gcgctccgtt ttggggtctg tgaaggccgt atttcaggag tttccggtac aatgtattgc
     5581 gcggcatggc gatggcgcgt gccacctcgc tgatattcca gcgcttcgat tccaggtgcg
     5641 caagaatctt ggcgcgctct tcttgctcaa aggctttgcg atctcgcggc ttggacttgc
     5701 tgtccacgga catggcagga aaaaggtcga ggtctgccaa accgatagcg tttccttcgg
     5761 ccaataccca cgcgcgtcgc aggagattgt cgagctgacg aacatttccc ggcaaaggat
     5821 gccgtagcaa cgctcgcaat gcttcaggac gcagcgtagc cgcctgtttg ccaagacttg
     5881 aagctgcctg atggagaata tgatgacata aggcaggaat atcctccgga cgttctcgca
     5941 aaggcggaag cctcacttcg acgacggcaa tgcgatagta aaggtcttct cgaaatcgac
     6001 cggcggaaat ctcatcgagc aggtgacgat tggtggctga aaccagacga atatcgatgg
     6061 ggatctcttg actggagccc aggggcctca cgcgccgttc ttgcaaaaca cgcaggagtt
     6121 tggcttgcat tccaaggggc atttccccaa tttcgtcgag aaagagcgtg ccaccacagg
     6181 cgctcaccag cagacctttc cgatccgtat gggcacccgt gaaagcgcct ttcaccacac
     6241 caaataattc agattcaagg agactttcgg gaagtgcagc gcaattgatg gataaaaatg
     6301 gcccttggcg acggggacca ttgaagtgca gcgcccgtgc aaccaactcc tttcctgttc
     6361 cgctttcgcc ttgcacgaga acatcgatat tcatgtccgt cacccgatcc agcgtgtgga
     6421 aaaccgcttg catcggggca cttgtcccga tgatgctgct gtaatcgtat cgatgttcga
     6481 gggcttgttg gcgcgtttgc acttcggaag aaagcttttc gatggtagcg gcctgacctt
     6541 cgagcaattg ctgcacgcgc tcttgttctt tttgaagcgc ctccgttcga cgttccaagt
     6601 cctctacgag gcgtgcattg cgcagggcga tcgccacctg gtccgcgaaa gctcccagca
     6661 agtggagcat gtcctcgtcg aattgatctt tccgaaagcg atggtcgaga tatatggccc
     6721 caagcacacc ttggggtgtg gttacaggta cgcacacaac cgagcgaagc cgcatcgcat
     6781 gaaccgattg atgggaacga aaacgatcat cggtaagagc atccgtggtg gtcacgggaa
     6841 gacctgtttt caccacgttc tcagcaatag accgactgaa cttgagatgg gaacggccga
     6901 tacgctcctt gtcgagattc cgcgcgacgg caacatcgag ttgtttgctg ttgggtttgg
     6961 agcggacgag caaaaaaccg cgttccgctc cggacagttc aatcgccgcg tccaacgcga
     7021 aagacagaac atcgtgggtg gcaagagacg aattgagccg tcggttgatc tcgaggattc
     7081 tcagaagttc gcgaggtgtt gggggagctg ccttgagtga tggctcggga cgatgggcgc
     7141 tgcggcgggg atgcgcccaa aatgccggac gaaaagactc cggaagaccc accgccatgg
     7201 attcccaagc cctgcgcgct tgcagacgat ggtcacaagc gagttggctg gcgccttgct
     7261 gctcataaat atcggccatc aacgtttgca tttcagcgtc gagcgcggac tgttttgacg
     7321 ctgcggccga gatcagggct tgttgtgcat gagacagggc ttgtgcggga tgacgaccga
     7381 gcagcgatcg agccgacaga gcgtgccagc gagcggtaag gtccggggca gagatgtcgc
     7441 tcagggtttg gaaaaggctt tgtcgcaagg aatccgcctg ggcgtggttg ccttgttgga
     7501 gcagtaaaga ggcttggtgc cagcacattt cggctacttc ccgctgggca ccctgctgcg
     7561 aaaatagccg ttccgcacgc tcgtatcctg tcatggcctt gtcgatcaag ccttccgcct
     7621 cgaaaagttc ggcttccaga gccaggcgat tggcttcgac ataaccaagg tcacgtgtgt
     7681 ttcgaagcgt agaaagcagg gtgcgagccc ggtcatacac accaattcca gcataaagat
     7741 tggcaagatt cagcgttagg gtgcaagccg tattgccttt tccaatggcg cgcgcaatca
     7801 aaagtccacg ctcgtacgat gccaatgcgt ttccccattc tcctgcctgt tgttgcgctg
     7861 ttcccaggtt catggccgtc gaaaccatct ggtcccccaa accatgctct tccgcccaac
     7921 gcgacgcttg acggtaaccg tcgatggcgc gcggcagatc cccctgtcgg taggccagga
     7981 tcgccgaaaa cgacgaaagg cgtacttttt ccctcggtgt gagcgcgtca gggttcgccg
     8041 tatttgcttt ttcaagatgc tcgacggctt gctccatttg tcccaagtag gtgcaggcca
     8101 ggcccatact cgcgtgtagc ttcgcccggg tcgaagcttc cgacgtgaga tgcaagccaa
     8161 ttttgccgtt ggtgagcacg tgttcgtagt ttccttgctg gattgcgatt cggcatctgg
     8221 tttcgagcaa gcaagctttg acgtccggct tctctgccag gaaaagagcc gtattgatgg
     8281 ctgtttctgc tcgttgaagt ctgcccgacg ccagaaaaca taaggctgct tccattcggc
     8341 aggcgatgcg cacggatacg ccaggacgag agcgaagaca gcgggcgatc cagcgcaagg
     8401 cttgctcaaa gcgtccacac aagcggttga cttgtgcgca cgccacctgt acttccggtg
     8461 gcaccctttc gccaccagat acggcttctt ccaaagcatc ggtgactgga cgccacgctt
     8521 gcggagactg ctcgatcaaa gacgcgtatt ccagcagcaa ttgtcctgct tggaatgatt
     8581 cggccccgca ggcgaagtga taagcccgca taggaggatt atccgcgctt ttttccgcat
     8641 gccagcgatg aatgcttgtg acggtctccc tgtcgataga ggcaagaata cggggcgcga
     8701 tccaacctcg ttgcagtttc caatggcgcc cctcccgttc gatccatccc ctttgatgaa
     8761 gacgcaacaa gacgtgtgaa gttgtctgac agcaagagcg cagggtggca tcatcacaca
     8821 tcgcgaccgc aagccacccc agcacgagtt tttcttcttt cggcaaggaa aggaataagc
     8881 ctccggccga ttgcggggcg gacgcgtggg gaaggtctga ttcaggaatc tccccccgca
     8941 aaaccctgtc cataagagct tccagatcag caggataccc accagtgagg cgtaggacat
     9001 ggggcacaac ggctggactg agcaagcctt gggtccacgc caagacacct gaagcatcga
     9061 ggggcccaag ggaataagtc gtgatttcct gatttttcaa ggttggctcg tgccgactcg
     9121 tgagcaaaag gagccaagga tccgtgggtt cgaggagccg gagccaggcg tcgagcaact
     9181 cgcgctggtc ggaagcaaga aggtggatgt catcgagcac cagcaccacc ggctgggaac
     9241 gttgcgcaag ctcgtggcgt gtctggacga tggcggacaa gccctgacgg tcaatcgcgg
     9301 aagatccaag cgctcgcgcg aacatatctt cgatagcacg gggcgctcga gcatcacctt
     9361 gtatgactgc gccttcaagc tgagcttgcc atttcatctc gttcagcaaa cggcttcgtc
     9421 caacgccatc cgggccgtga aaaacgagac atcgagggtg gggagcgcgt tgccgcaagg
     9481 cgctaagggc ctcttgaaag gatttcaggg gagcctgtct tccgacgata cgctccagtc
     9541 gtccttgagg ggggccgagc gtaaaaggtg gaaaaccaaa ggcttcattg acttcggcgg
     9601 catcgctggg acggtggtcc ggattagggt cgatcagccg ttggagcagg ttggagagtt
     9661 cgggcgcagg agttggcaag acgatggagc aggtgatacc gaaagcgaaa agatcggcac
     9721 gaatgtcgac cgggcctcct tcgagcagtt ccggagccat gaaccctagt gtgcctgata
     9781 tggtatccaa ggtggctttc aatggccgag cgcaaccgag gtcgatgagg acgccctgtc
     9841 cctggggggt gacgaggatg ttatcgggtt tgatgtcgcc gtgccggatt cccaggcgat
     9901 gcaggaaatg cagggcatcg agaatatggg cgaggggcgt gagaatatcg tgggccgaac
     9961 gggactgggc ccacggcgtc aggggggtgc catcgatgaa gtccgccgtg taaaaaaggt
    10021 ggtcggcttg tttttcgagg tcacggacga ggggtaggtg agggtgaaac cgtccgacga
    10081 ggattgcgaa ttcttgccgc agggccgcgg tgagggcggg gtcatgggaa tgaaggagtt
    10141 tgagggtgac gaggtcattt cggagcaggt cacgagcgag taaggcagag gaagaggctc
    10201 cttttcctag ggggtgaagg atttcgtagc ggtgttggag gacggtgccc tgcttgatca
    10261 tgtctcacac tgtaccaatt gcaccttagc tgctcaattt ggtacaaatt ggtacaatac
    10321 gacaggagag tcaagcgaaa aaacaccgtt cgagcccccc cgtacttggc aagcactttg
    10381 cattcatcag gcgctaggcc ctgcggctca taagggttaa ccatgagaag acgcgcggtg
    10441 cgggctgtcg gaccgatggg gtgacacgcg cggatagtgg aaacgatccc atcgaaatag
    10501 gagtcctacg atgaaaacga caaacctttt cctctccctc gctcttgctt tgccgctgta
    10561 tgcttgcgga agcgatgcat cggacgaaga tatcaacggc tcatgggata agctgactcc
    10621 cagagtctgc gaatatgacg gcaaatccta caaggtgggc gactcgttcc cggctgccga
    10681 tggctgcaac tcctgcgcat gcaacgaaga tggctcggtg gtctgcaccc tggtcgattg
    10741 tgaacgcgtt acctgcgaat acaacggaaa atcctacgag gtgggtgact cgttcccggc
    10801 tgccgatggc tgcaactcct gcgcatgcaa cgaagatggc tcggtggtct gcaccctggt
    10861 cgattgtgaa ctatcctgaa tctcaccgaa aatggtgccc ccctgggctt ccttgttttt
    10921 tgagcgccct tgccgcaacc ggacccgcga atcccccacc atcccttctg cccaacgtct
    10981 ttagctgctt atccacggca accggccgat cagtccgcgc atcgttccca ccgcggcaac
    11041 caagggccaa agcaaaggcc tgccatgagt cgttcggagg ccaattcgta cgattccatt
    11101 gccaatctcg ttgaaattcg gggcaacata aggccttttg tgtagctaaa accacgacta
    11161 tccttgcttc cgtaccaaac gctttggaaa aacggctgaa gatacggtcc ccggcaagcg
    11221 ttgtcccgcc gcgtggtttc tgctcctgag cgctgcgttg gcaggacagc ggctcgaggt
    11281 tgcggaaatg gcgagaatgc gtcagaaaaa cgcatgaatg taccatagtg ttcaaattcg
    11341 ttggcacatg ctcatgatgg tacaacatgg tacatacttc cgatcgggaa tcccgtgcgt
    11401 gagcagcatt gtgaattggc aagacttttg ctctactatt ttggaaatgg cgcaccgtct
    11461 ctccccagtt ctgcttgtcc tcgtcaccct gctcgcgtgc gttcattgtg ggatcaatcc
    11521 tgttcccgaa ccgccctccg gcgagcccat cgccccccct gccatcgaca atatcaccgt
    11581 ctatcctttc caaatcatga cagaagggaa tatcagcatc cacgcaggcc cgaatacagc
    11641 ggaacctgga agcacgctat gggcgatgaa tatcgatggc accggccctg ccgtgaccac
    11701 caaagtccac gatgatggct cttttgcctt agagattacg gccggatccg gtgatgaagt
    11761 gcggttgcaa gtccgatcgg gccagcagcg ttccaagccg attgatgtga taattcccag
    11821 tgaagaaggc cctccaaccc tcgccgtgag gcccctggca ggctgccttt ccctcgagcc
    11881 ggctttcgag ttggaactgc cgccacccgc gggctcgcag ccgccctccg cctcgattct
    11941 catcaaaaat cgatgctctt ctccaatatc caccttcgcg tcccttccga ggctttcgcc
    12001 gtgctgcctt cggaattgac gatcgcccca ggaaacaccg ctgaagttgt cgttcaggtc
    12061 ttcggcgacg ctccccgaga agaaattctc ctgctcgaaa cccaagctcc tgagctggac
    12121 cgtcgaccca tcaccctttt cggccaagga ggcattcatg gccttttgcc caactgctcc
    12181 cttgtgggag gccgttgtct ctcagacccc atggatgtca cgtttccggc caattgtgag
    12241 gccttgggaa tgaaatacct cgaagccact tgccaagcga tgaacctgag ctgctgtgga
    12301 gatgctccct gattcagcct gtcacgaaaa aaggatactt gtcatgaaac tagcaacaaa
    12361 ttggatggta ctggcccttt ctttcccctt gtttgcttgc agcggtacgg tttcgaacga
    12421 gggaacccat agcgcgtcag agaaattatc tcgtcaggaa gccaaagcct tggacaaggc
    12481 agattggccc accgactact gcgaagtcaa cggatggtac ggtgatgggg tgtattgcga
    12541 cgacttctgt cctttgcctg atccggattg cgcgcagtcc tgtgcctcgc ttggaggcga
    12601 atgcttgtcg gaccccatgg acgttggctt tggcgcaaac tgtgaacagc tcggacgccg
    12661 caccctgacg ggagcttgtg aagccttcaa ccagacctgc tgcagcgccc cttcgtcgtc
    12721 gaatcccacc tgcgaagaga tgggcggcac ctgcctgtcg gaccccatgg acgttggttt
    12781 tggcgcgaaa tgcgaacagc tcggacgcgt gactatgtcg ggccgttgcg aagccatcaa
    12841 ccaaacctgc tgcagcgaca agaaagaaag aacgcctgta gaggagcatt gcatcagaaa
    12901 tgcaggcgat tcctgcgagg cggatgcaga ttgccaaacc ggggggtgtg gcggagagtt
    12961 atgttacaac ccggcgttcg agggggggat aagcacctgt gattgcgccg aaccacagcc
    13021 acctttggtc gctggatgcg gctgtgtcga cggtgtctgt tcctggtata agtgaatcaa
    13081 tcactcatct ctgtcctttc gttcccgcga aaacacagcc ggtcgacggt cttcgcgagt
    13141 ttcgcaaaac cgtgtgaggc gctggcaaaa gcgggggctg ctcgccccta tcagcgaaaa
    13201 aaagcggtgg aaaactcgtc ggtgggaacg agaagcatcg aatttctctg cccctcgagc
    13261 ctgataagtg tgtatcccga ttccacacaa gacccccatg gtaaccaaca aatataccga
    13321 attcctcctt ttgcctcctg gcgtgttggc gctttgaggg gtggccgtgt ttgtcgtttg
    13381 acaaaatcaa tacattccgt taacatacga gtctattcgc catgaccaag gattcagaaa
    13441 cgatgtctga tgacaaaatt gaacacctga tggctcagtg ggggttttcc aatgtaaaga
    13501 aacccattct tcctgacgaa agaatcctag ccaaagtatt tgaacatttt cgtgaattat
    13561 caaaacaccc tgaaatagcc gcaatcgtaa aacgacaaag cgacggagaa ataacccgta
    13621 aacaaatgtg ggacgacatg agagccgtag ccaaagaaat cgacaaagcc aataagaaat
    13681 aatttgatac tgtcgaaatg atgttcctat cgccgtcctc attattttat ggcgataccg
    13741 tatttacgaa atatacgtaa ccgatcgacg ccggggctgg ctattagcat tatcatgaaa
    13801 attcgttaag accatctacg cagccatcca gttccgcact ccttacctga ttcttgcctc
    13861 accatggact ggattgtcga acctacacct gagtttcaac ccggtcttgt ccgtggcatg
    13921 gttgctggtg atgaagggca ctggggttgt tcgaagcaag tcatcacggg ccgcatcg
//
LOCUS       DAOSOR010000076        13971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4397_length_13971_cov_5.114041, whole genome shotgun sequence.
ACCESSION   DAOSOR010000076 DAOSOR010000000
VERSION     DAOSOR010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 13971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13971
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4397_length_13971_cov_5.114041"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PK710_06085"
     CDS             <1..208
                     /locus_tag="PK710_06085"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOT09318.1"
                     /translation="PLEWEGLRALVEASTIPMIAIGGIDERTAPLIREAGCAGIAVVS
                     AVMKAEDPRRACMGLRHAFEYATP"
     gene            375..1115
                     /locus_tag="PK710_06090"
     CDS             375..1115
                     /locus_tag="PK710_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09319.1"
                     /translation="MMSLRNRPAKPFASSLSPSYPRMLGIGLIAFATACGGTVDDPGA
                     GASGAAGSSGSAGSGGGQAVAEICDNGIDDDNDGDVDCADKDCADALNCQHTAGVAPP
                     PFEVCDNDVDDDGDGDVDCADSDCADTPMCQNIGGGVPYPYEICDNGVDDDNDGDIDC
                     MDSDCFDAPECQLAGEVPSSFEICNNGIDDDGDGDIDCADSDCLGAPECGPAGAMPMP
                     YEICDNGIDDDDDGKVDCDDPDCATECK"
     gene            1131..1493
                     /locus_tag="PK710_06095"
     CDS             1131..1493
                     /locus_tag="PK710_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09320.1"
                     /translation="MAERFLLNRILWTFFRKRGRVLRAMDDSGLKARILARRNRFLAL
                     AVSGAGVVACSPTPRAEPATGPVIVTVDPPEQPKDASADSVGDAAVQPEATFHSPRTE
                     QAPDFVPVICLSFDPDLP"
     gene            complement(1500..2201)
                     /locus_tag="PK710_06100"
     CDS             complement(1500..2201)
                     /locus_tag="PK710_06100"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOT09321.1"
                     /translation="MARNISRILDEQIQRWNLEQKFRARAQEPTEFRAQRVITLSNAI
                     GSNGIAVAHKIGEMLHIPVYDREIVEHIATTEKVRVEAVESLDQRALGAVDDYLVNLF
                     RERNFDQSDYMKALTKTITSLWAHGSCIFIGRGASHIISRRFCLAVRTVAPWKHRVRR
                     VQDLTNMTKEQAEAEVLRIDGDREYFIRRWFKLGIEDPLSYDLTVNTAGLSTQAAAQL
                     IITAFQQKFVNVEDT"
     gene            2296..3435
                     /locus_tag="PK710_06105"
     CDS             2296..3435
                     /locus_tag="PK710_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT09322.1"
                     /translation="MLRFAHVSDLHVGHLSANLIRLGRLVLPRSRGEGAWDIATRLLA
                     SSWFERRSLFEPLLRSTHLAHDYQTKNLVAVVQSAREHGCQHVILTGDIANLGAASEM
                     REARLVLSAFGYEGDSLTVVPGNHDVINFQGVAEFRSVMAQPPWPHLRWLAPDVVALA
                     LDSTVHRPDLDWRDAIGMNARGTFSDDMVAKADELLSTVPKDAFKILCCHHHLVDLPP
                     DGYVDDWSGKLDPRLAGPAEHASDILDLAEKYAVGLILFGHRHRATHHLFTIRGIPAA
                     CSGSVTEVQRQGNLRYRVFDLEGGRIRRRYWVDTYPENASREVVEKALRGISSVETDD
                     SLKLTIPLREGQARYDMARLQEKRRALDEKVLEKVRKRLLSEQKR"
     gene            complement(3475..5124)
                     /locus_tag="PK710_06110"
     CDS             complement(3475..5124)
                     /locus_tag="PK710_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOT09323.1"
                     /translation="MAATLHAQDISLSIAGTEILRRASIALFPAKRLGIVGPNGAGKS
                     TLLRVLAGWLTAEEGTITLSPPRATVGYLPQEPDRLPEETVLAFLMRRTGVAEAAKRL
                     ETTTVALAEGQPGSEEAYAEALERWLNLGGGDLEARAEIVLQELGILKDFMNLPTDRL
                     SGGQAARVSLAAVLLSRFDILLLDEPTNDLDFDGLSRLEDFASKTDAALAVVSHDRAF
                     LERVITDVLELDAHTRTTRLYRGGWNAYLENRALAQRHAQQDYEVYRDKRDTLAERAQ
                     QQRLWAMQGVRKAKGDSSEKDKHIKHFRIASTEKLAAKARATERAIERLEVVDKPWQG
                     WELRLQIARAERSGDVVARLQQAVVRRGSFTLGPIDLQIRWAERLAIVGPNGSGKSTL
                     LELILGRCEPEQGTRWLGPGVIVGELEQRRGRFMNEPSLLDSFTEASGLLPREARTLL
                     AKFGLGADHVGRAAACLSPGERTRASLALFQARGVNCIVLDEPTNHLDMPAIEQLESA
                     LATYDGTVLLVTHDRVMLERFGATRTIEVVHGTIRERDMEG"
     gene            5202..6365
                     /gene="ruvB"
                     /locus_tag="PK710_06115"
     CDS             5202..6365
                     /gene="ruvB"
                     /locus_tag="PK710_06115"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOT09324.1"
                     /translation="MKTAKMQEQDLFDSVAGSDFSTPMSMTKSRGKLKARDQDADLPE
                     HDRLIAPTKREDDARFDRLCRPSSLDEYIGQEKHKSNLRVFVEAARRRGEPLDHVLLC
                     GPPGLGKTTLAYILANEMGVQVHTTSGPAIEHKGALAGQLTKLESRDVLFIDEIHRLT
                     ASVEETLYPAIESFRLDVMMGDGPHASSIQLDLNPFTLVGATTRTGLLTSPLLSRFGY
                     IVRLDFYPVEELVQIVKRSAMLLNVEIDEKGAKAIAQRSRGTPRVANRLLRRVSDFAL
                     VLGNGKVDQTIAREACERLGIDEGGLDEMDRRLLSIIIEAYEGGPVGLDTLAAAMAEP
                     RDTIEDVYEPFMLQQGFLGRTPRGRIATRKAYEHLGIKRPEGKGGSDPQETLF"
     gene            complement(6379..6834)
                     /locus_tag="PK710_06120"
     CDS             complement(6379..6834)
                     /locus_tag="PK710_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09325.1"
                     /translation="MKLLGNTTVRWVALLAMSCSRDPSASGTNHASESSVDEPSYRLT
                     IEAPGPFEKDKEATALVRLTAKGDYKVNAAYPFRFRCQDRPEGLRYTTAELERKDGKL
                     DEKTAEFSLPFVPMEAGNLKVGGVLSLSVCTEAKCIMDKREVVASIAVP"
     gene            complement(6946..9492)
                     /locus_tag="PK710_06125"
     CDS             complement(6946..9492)
                     /locus_tag="PK710_06125"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT09326.1"
                     /translation="MPPMPPTPRPKAPPPGKKNLPPAALGRRIGRYSLLRLIARGGMG
                     EVYLAATTGIEGAERPCVVKIIRREHASNSSFVARFLDEARVQAQLHHPGVVQVIEAD
                     TDEQGEPYVVVEYVEGRSLGEVRSRCADVSYRLSWAEAVALAASTAEALAHIHERVDV
                     SGQPLSIVHRDMSPQNIMVGYGGDVKLIDFGTALGENRRSHTVAGVVFAKPGYVAPEV
                     ANGNTGNALVDIYALGVILWELLAGRRFLQGEASDHLAKVAKNQCPLPKLASLVNAPA
                     ELDDILAKLTAFHTKDRYPNARSAHRDLARLLSQAPSLSNGERGMRARISQLMNTLYP
                     AEPTSSRSLFAKLVAQARGHHNQENRPPIECEEPVRDTETDDQLLPGTRYRMLGKIGE
                     GAMGHVFEAEHVDLGRKVAIKVLAAHHATSSDYSARFRREARTLSRLSHPNLVTLHDF
                     GQARDGRLYCAMERLEGETLDKVLDREGPLPWKRALRLGAQAASALREAHRAGIVHRD
                     LKPSNLFLCEDGTLKLLDFGVAKKGDESADSNKDLSSFTLFGTPEYMAPEQVTAGQVD
                     ERADVYALACVLYEMVTGTLPIAAKSTVALLDAKTKEDPEPCRDRTPLRHIPIEVDRL
                     ILRALARRPEKRTPTAARLHDEILAALESPEKTRRRRRTLGRYALFLSITATVLALSA
                     GMVRRYPEMIPRARAALVAALSADTASTTHESSASFTASPQLPAHATPVAITAAVTNP
                     PISLQPLEANPISPSEPSAQERDWTEAIQAARQWADHSSSRPSRLLLARLLLHSGKPQ
                     QKLEARALLEELQRQDPSCTETQSLLATATSGALHSSTPSKPQTSPVAQR"
     gene            9812..10267
                     /locus_tag="PK710_06130"
     CDS             9812..10267
                     /locus_tag="PK710_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09327.1"
                     /translation="MWQRLSRLPPFVAMALSIFRTTIVFATLLVVLGWVRVATAYAPF
                     CDPRAATGVAFPPFTVIPDLRWEPSFEVFFLQSCERMPAYRSVEGIPHPAKDKMSEMQ
                     SGHGMDLAALSNSVDIPRRFRPTRVALSGQRTPNLQEILHRVYRPPKHL"
     gene            10323..12272
                     /locus_tag="PK710_06135"
     CDS             10323..12272
                     /locus_tag="PK710_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOT09328.1"
                     /translation="MNKGTAIVGFILSFLAGMMLMWGINQSSGRTGSESIKADKAGKW
                     SDEDSPISVTSEDPVWGNRNAPVTMVIFSEFECPFCARVEGSIEAIKQQYGPDKVRVV
                     WKSNPLAFHKSARPASVAAETVRALAGNEAFWKFHKSAFANQKALTRENFEKWAQEAG
                     VDMAKFKDALDKETYAAKVDKDLALGQKLGVRGTPHTFVNGVTVNGAQPVDKFKEVVD
                     AQLAAAQAMIKAGTKPDKVYVEASTKNFQKPAEQPKNEREAPAEDKTVWKVTIDKSPS
                     KGPTDALLTLVIFSEYQCPFCKRVEPTIDQLMKDYDGKLRVVWKDRPLPFHKEAEPAA
                     LFALEARKQKGDKGFWDAHNLLFENQPKFQMEDFEQYAKTLGLNWTAVKNAIEKKTHA
                     ASIETDVALADGLKASGTPHMFINGRRIVGAQPIDAFKKLMDEELAKAEAMVKGGTAK
                     ASVYETIMKTAQTPPPPPPPEKKDVPAPTAQNPYKGGKNAKIVIQLFSDFECPFCGRV
                     EPVMEEILAKYGDKVKVVWRQFPLAFHKNAKLAAEASVEVFKQKGDAAFWKYHNTLFQ
                     NQKALERADLEKYAEELGCDMAKFRAALDNHTHAKFVEDEIAVAEKAGIRGTPASVIN
                     GYFVSGAQPFAQFEKILKLAESELK"
     gene            complement(12358..>13971)
                     /locus_tag="PK710_06140"
     CDS             complement(12358..>13971)
                     /locus_tag="PK710_06140"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT09329.1"
                     /translation="VLQQGPMHPRRVIHIGKQIATALARAHGLGIVHRDLKPENIMLV
                     SRDTDPDFVKVLDFGIAKVPVSQISKEAGKQHKTLTKVGMVYGTPEYMAPEQALGQDV
                     DARADLYALGVILYEMLVGRRPFEADNPVALLGMQVTQPPPAFRQVAPGLHLPQEIEA
                     IVMELLEKEAARRPSDAKQVRDVFEILEQNLDTSSWTEDARSNVSAAMSLTHAPTVFE
                     GIASAKPRAPSIASVMARISAWSSQLAQTLRAADAQAQPHRWQAALPAPLRTLKLSVW
                     LGLAAGVGMLALIVVIGIVWWVRTPSTVKPVFDETSWASPPVASSMQNASATADPQQL
                     EKAVKAGSESIEQLLTQFPNDPAVLAQVAKARLGGASPDTCIEVYDKLFSLDPEKRKD
                     NAVLEDVLGALTKPSSQEVVFSLLESHWGTEGPDLIHDLAFGKRTTTAAIASRAAKSI
                     KSEAARKNASPALLIALDLRFNGGCQAKHRLLDRAREHGDERSLTQLRGLLHAKGCGF
                     LGLSDCWKCMRADRKLNDTIKAISERTKQ"
BASE COUNT         2836 a         3780 c         4337 g         3018 t
ORIGIN      
        1 gcctttggaa tgggaaggat tgcgggcgct ggtggaggca tcgacgattc ccatgatcgc
       61 gatagggggg atcgacgagc gaacggcgcc cttgattcgt gaggccggat gcgcgggaat
      121 tgctgtggtt tcggcggtga tgaaagccga ggatccgcgt cgcgcgtgca tggggttacg
      181 ccatgctttt gagtacgcaa cgccatagcc tggatggggg tagaggtacg ccattgggtt
      241 tcggtaatct gtgtcaggtt gagggcccgg gctctggcac aaattgactc aaatggattg
      301 gtactatcga aatcgcgctt gaatcaatgg ttgttctgtt ggcctgaagg atgcatagtc
      361 gcggggtgga ggtcatgatg tcattgcgta atcgtccggc gaaacccttc gcttccagtc
      421 tatccccttc atatccccgt atgcttggaa ttggtttgat tgcttttgcc acagcctgtg
      481 gtggcacggt ggatgaccct ggtgccggcg caagtggtgc cgcgggttcc agtggcagtg
      541 ctggctccgg tggggggcag gcggtggccg aaatatgtga caacggaatc gacgatgaca
      601 acgatgggga tgtggattgc gcggataagg attgtgccga tgctctgaat tgccagcaca
      661 cggccggggt agcgccgcca ccgtttgaag tttgcgataa cgacgtcgat gacgatgggg
      721 atggggacgt cgattgcgcg gatagcgatt gtgctgatac gccgatgtgc caaaacatag
      781 ggggaggagt gccgtatcct tacgagatat gtgataacgg tgtcgatgac gacaacgacg
      841 gggatatcga ctgcatggat agcgattgct tcgacgctcc cgaatgtcaa ttggcagggg
      901 aggtgccgtc ttcgtttgaa atctgcaata acggcatcga cgacgatggg gatggggata
      961 tcgattgcgc ggacagcgat tgtctgggag cccctgaatg cgggccggcc ggagccatgc
     1021 cgatgcctta tgaaatctgc gataacggca tcgatgacga cgacgacggg aaggtggact
     1081 gcgacgatcc cgattgtgcg acggaatgta agtagaaccc gaagtcgtgg gtggctgagc
     1141 ggtttctgct gaatcgtatc ctctggacgt ttttccgaaa gcgaggcaga gttttgcggg
     1201 ccatggacga ttcgggtctc aaggctcgca ttcttgcgcg cagaaatcgt tttcttgcgt
     1261 tggccgtcag cggtgcaggg gtagtggctt gttcacctac cccccgtgcc gagcctgcga
     1321 ccgggcctgt catcgtgaca gtcgaccccc ctgagcaacc gaaggatgct tccgcggatt
     1381 cggtcggcga tgcggccgtg cagcccgaag ctacttttca ttccccccgc accgagcaag
     1441 ctccggattt cgttcccgtt atctgcttat ccttcgatcc ggatcttcct tgaacggggt
     1501 caagtatctt cgacgttgac gaatttttgt tgaaacgccg tgatgatgag ttgggctgct
     1561 gcctgggtgg atagccccgc ggtattgacg gtcaagtcgt agctcagggg atcttcgatg
     1621 ccgagtttga accaacggcg gatgaagtac tcccggtctc catcgatgcg cagcacctca
     1681 gcttccgcct gctctttcgt catgttggtc agatcctgca cgcgacgaac acggtgtttc
     1741 cacggagcaa ccgtccgaac cgcaaggcaa aatcgtcggg agatgatatg cgatgcgcct
     1801 cggccaatga aaatgcacga accgtgcgcc cacagcgatg tgatcgtctt ggtcagcgcc
     1861 ttcatgtagt cactctgatc gaagttacgc tcccggaata gattgactag gtagtcgtcc
     1921 acggccccga gcgcccgctg gtccaacgac tcgacggctt ctacccgcac cttttccgtg
     1981 gtggcgatat gctctacgat ctcgcgatcg tatacgggga tatggagcat ctcgccgatt
     2041 ttatgggcta ccgcgatacc attggaaccg atggcgttgg ataaggtgat gacccgctgc
     2101 gcccgaaatt cggtaggttc ttgtgcgcgc gcccggaatt tttgctcgag attccaacgc
     2161 tgaatttgct cgtccaatat ccgactgatg tttcgtgcca tgagggcccc cgtcgtttgc
     2221 atcctgtttg tggtagtgct tcacatgatc tcagattaat cccaaccacc ccctctgccc
     2281 agtctgtaaa attcgatgct tcgtttcgcc cacgtatccg acctgcacgt cggccaccta
     2341 tccgccaatc tgattcgctt gggacgcctt gtcctgccca ggtcacgggg ggaaggcgcc
     2401 tgggacatag cgactcgtct tctggcctcc tcgtggttcg agcgccgaag ccttttcgag
     2461 cctctgctgc gttccaccca tcttgcccat gattatcaaa cgaaaaatct cgtcgcggtg
     2521 gtccagagcg cccgggaaca cggatgccag cacgtcatcc tcaccggcga catcgccaat
     2581 ctcggcgccg caagcgagat gcgcgaagct cgtctcgttc tatccgcatt cggatacgaa
     2641 ggtgattcct tgaccgtggt tcccggcaat cacgacgtga tcaacttcca gggggtggcc
     2701 gagtttcgaa gcgtcatggc tcaaccacca tggccgcatc tgcggtggct tgcccccgat
     2761 gtggtcgccc tggcgctgga ttcgacggta catcgtccgg atctggattg gagggacgcc
     2821 atcggcatga acgcgcgcgg aactttttcc gatgacatgg tggcgaaagc cgatgagcta
     2881 ttgtctacgg ttcccaaaga cgcattcaaa atcctctgtt gtcatcatca tctcgtggac
     2941 ttgccacccg atggttatgt cgacgactgg tcgggaaagc tcgatccccg gctggccgga
     3001 ccggcggaac acgcctccga tatcctcgat ttggccgaaa aatatgcggt aggcttgatt
     3061 ctcttcggcc atcgtcatcg cgccacccac cacttgttta cgattcgagg gattcccgcg
     3121 gcatgctcgg gctccgtcac ggaagtccaa cgtcaaggaa acctgcgcta tcgcgttttc
     3181 gatctcgagg gtggtcggat tcgacgccga tactgggtcg atacctatcc ggagaatgca
     3241 agtcgggagg tggttgaaaa agcgctgcgt ggcatctctt cggtggaaac ggacgatagt
     3301 ctcaagctga cgattccttt gcgcgagggc caggcacgct acgatatggc gcggctgcaa
     3361 gaaaaacgtc gtgctctcga tgaaaaagtg ctcgaaaaag tgcgtaaacg tctgctgtcc
     3421 gagcaaaaac gatgaccacc ccccttgcgg ttcatggcgc gcttcggtct gcgctcatcc
     3481 ttccatatcc cgttctcgta tcgttccgtg gaccacttcg atggttcgtg tggcgccgaa
     3541 tcgttcgagc atcacacgat cgtgtgtgac caacagcacc gtgccatcgt acgtggcaag
     3601 agcgctttcg agttgctcta tcgccggcat atccaggtgg ttggtgggtt cgtctaggac
     3661 gatgcaattg actccgcggg cttgaaataa ggcaagggaa gcgcgagttc gttctccggg
     3721 ggaaaggcaa gcggccgcgc gccccacatg gtccgcccca agcccaaatt tcgcaagaag
     3781 cgtccgggcc tcgcgcggga gcagccccga ggcttccgta aaactatcga gcagcgatgg
     3841 ctcgttcatg aaacggccgc gccgctgctc gagttcaccc acgatcacgc cggggcctaa
     3901 ccacctcgtg ccttgttccg gctcgcatcg tccgaggatg agttcgagca gcgtggactt
     3961 tcccgaacca ttggggccga cgatggccag ccgttcggcc catcgaattt gcaagtcgat
     4021 ggggccaagc gtgaaagaac cacgacgaac cacggcttgc tgtagccgcg cgaccacatc
     4081 gccgcttcgc tcggcccgtg cgatctgcag gcgcagttcc cagccttgcc atggtttatc
     4141 aacgacttcg agtcgttcga tggctcgctc cgtagccctg gctttggcgg cgagtttttc
     4201 cgtgctggca attcggaagt gtttgatatg tttgtccttt tcgctggaat cgcccttggc
     4261 tttccgaacc ccttgcatgg cccagagccg ctgctgctgc gcgcgttcgg ccaaggtatc
     4321 gcgcttgtct cgatacacct cataatcttg ctgggcatgg cgctgggcaa gcgctcggtt
     4381 ctcgagatag gcgttccatc caccacgata gagccgcgtg gttcgtgtgt gggcatcgag
     4441 ttccaacacg tcggtgatga cacgttcgag aaatgcgcga tcgtgggaga cgaccgcaag
     4501 ggctgcgtcg gttttcgacg caaagtcttc gaggcgtgac aggccatcga agtccagatc
     4561 gttggtgggt tcatccagca acagaatgtc gaagcgagac agaagcacgg ctgccagaga
     4621 cacgcgagcg gcctggcctc ccgacaggcg atcagtaggt aagttcatga aatctttgag
     4681 tattccaagt tcctgcagca cgatctccgc tcgtgcttcc aggtctccgc cccccaggtt
     4741 gagccagcgt tcgagggctt cggcgtaggc ctcttcgctg ccgggctggc cttccgccag
     4801 tgccactgtc gttgtttcca atctcttggc agcctcggcc acgcccgttc gacgcatcaa
     4861 aaaagcaagt acggtttctt ccgggagacg gtcgggttct tgcggcaggt agcccacggt
     4921 cgcccgtggt ggcgacaggg tgattgtgcc ttcttcggct gtcagccaac cggcgagcac
     4981 gcgcagcagc gtggactttc cggccccatt gggtcccacg atgccgaggc gttttgcggg
     5041 aaatagcgcg atggaggcgc gtcggaggat ttccgttccc gcgatgctca acgaaatatc
     5101 ctgcgcatga agagtagcgg ccacgaggct tcctttctcg agttggtctt tgggcagggg
     5161 ccgtacgacg ccaagtcgat cgagctgaaa tccgccttgg cgtgaaaacc gccaagatgc
     5221 aagagcaaga cttgtttgac tccgttgcgg gttcggactt ttctacgccc atgagcatga
     5281 ccaaaagccg cggcaagctc aaagcgcgcg accaggacgc ggatttgccc gagcacgatc
     5341 gcctcattgc gccgacgaaa cgagaagatg acgctcgttt cgatcggttg tgccgacctt
     5401 cgagtctcga tgagtacatc ggacaggaga agcataagag caatctacgg gtcttcgtgg
     5461 aagcggctcg tcgacgggga gagcctctcg atcacgttct tctttgcggt ccccccgggc
     5521 ttggtaaaac cacgctggcg tacatcctcg ccaacgagat gggggtgcaa gtgcatacta
     5581 cctcggggcc tgcgatcgag cataagggtg cgctggccgg acagctcacc aaactcgaga
     5641 gtcgcgatgt tcttttcatc gacgagatcc atcgcttgac ggcgtcggtg gaggaaacgc
     5701 tctacccggc gatcgagagc tttcgtctcg atgtgatgat gggcgatggc ccccatgctt
     5761 cctccattca actcgatctg aatccgttca cgctggtagg agccacgaca cggacgggat
     5821 tgctcacctc gccgctacta tcccgattcg gatatatcgt tcgcctcgat ttttatcccg
     5881 tggaagaact cgttcaaatc gtgaaaagaa gcgctatgct gctgaacgtc gagatcgatg
     5941 aaaagggcgc caaagccatt gcccagcggt cgcgaggcac gccgcgggta gcgaaccggc
     6001 tgttgcgtcg tgtgagtgat ttcgccctgg tgcttggaaa cggaaaggtg gatcaaacca
     6061 ttgcgcgcga agcctgtgag cggctcggca tcgacgaagg gggcttggac gagatggatc
     6121 ggcgattgct ctccatcatc atcgaagcgt acgaaggagg gcccgtaggg ctggatacgc
     6181 tggccgcggc aatggcagag ccgcgagaca ccatcgagga cgtgtacgag ccgttcatgc
     6241 ttcaacaagg ttttttgggg cggacgcctc gggggcggat tgccacgcgc aaggcctacg
     6301 agcatctggg aatcaaacgt ccggaaggca aagggggaag cgacccgcag gaaacgctgt
     6361 tttagacgga atatgccatc aagggacggc gatcgaggcg acgacttccc gtttgtccat
     6421 gatgcacttg gcttccgtac acacgctcag ggacaacacg ccgccaactt tcaggttgcc
     6481 ggcctccatg ggaacgaaag gtagtgaaaa ttcagcagtt ttttcgtcga gcttcccatc
     6541 ctttcgctcc aactccgcgg tggtgtatcg aagtccttcg ggtcgatctt ggcatcggaa
     6601 gcgaaatggg taggcggcat tgaccttgta atcacccttg gcggtcaggc gaacgagcgc
     6661 ggtggcctct ttatccttct cgaacggtcc gggcgcttcg atcgtcagcc tgtagcttgg
     6721 ctcgtcgacg gaagattcgg aggcgtggtt cgtgccgctc gcagacggat cgcgtgagca
     6781 ggacatggcg agcagggcga cccatcgtac ggtggtattt ccgagcaatt tcatgaggtc
     6841 gttgggaacg taagagcccc aggggcgcgg ttggatttcg tggggccaaa accgaggata
     6901 ttcatccgcg ggcttggggt gaagcgggat aggccgtggt tggggtcagc gttgggctac
     6961 ggggctggtt tgcggcttgg agggagtgga tgaatggagc gcgccgctgg tggcggtcgc
     7021 aagaagggat tgggtttccg tgcaagatgg gtcttgccgc tgcagctctt cgagcaaagc
     7081 cctggcttcg agcttttgtt gcggttttcc cgagtgaagc agcagccgag ccaacagcag
     7141 tcgggatggg cgagatgatg aatggtcagc ccattgtctt gctgcttgaa tggcctcggt
     7201 ccaatcccgt tcctgggccg atggttccga ggggctgatg gggtttgctt cgaggggctg
     7261 caacgagatg gggggattcg tcacggcagc ggtgatggca acaggggtag cgtgagctgg
     7321 aagttgcggc gatgccgtga aggaagcgga tgattcgtgg gttgtgctgg cggtatccgc
     7381 ggaaagggct gcaacaaggg cggctcgggc gcggggaatc atctctgggt agcggcgcac
     7441 catgcccgca gaaagagcca aaaccgtagc tgtgatgctg agaaacaagg catatcgtcc
     7501 gagggtgcgc cgacgtcggc gtgttttctc cggagattcg agcgcggcaa ggatttcgtc
     7561 atggagacga gcagccgttg gggttctttt ttcggggcgt cgcgcgagcg cgcgcaggat
     7621 gaggcggtcg acttcgatgg ggatgtgccg aaggggtgtt cgatcacggc aaggttcggg
     7681 gtcttctttg gttttggcat cgagaagagc gaccgtgctt ttggccgcga tgggcaatgt
     7741 gcccgtgacc atttcataaa ggacgcaagc cagagcgtag acgtccgcgc gttcgtcgac
     7801 ctgccccgcg gtgacctgct cgggggccat gtattcgggc gtaccaaaca aggtgaaaga
     7861 agaaagatcc ttattggaat cggcgctttc gtctcctttt ttggcgacgc cgaagtcgag
     7921 caatttcaac gttccatctt cgcatagaaa gaggttcgaa ggcttgaggt cgcgatgaac
     7981 gattcccgcg cgatgcgctt cgcgaagggc cgatgcggcc tgggcaccga gtcgaagcgc
     8041 tcgtttccac ggcagggggc cttcgcgatc gaggacttta tccagcgttt ctccttcgag
     8101 ccgctccatt gcacaataga gcctgccatc gcgagcctgg ccgaagtcat gcaaggtcac
     8161 gagattggga tgagacagcc gcgagagagt tcgcgcttct cgacgaaacc gagcggaata
     8221 gtcggaggac gtggcgtgat gagccgcaag gactttgatg gccactttgc gccccagatc
     8281 gacgtgttcc gcctcgaaaa catgacccat ggccccttcg ccgatctttc caagcattcg
     8341 gtagcgagtc ccaggcaaca attgatcgtc ggtttccgtg tctcggactg gctcctcgca
     8401 ttcgatgggg gggcgatttt cctggttgtg atggcctcgg gcttgagcga cgagcttggc
     8461 gaataggctc cgagagctgg tcggttccgc cgggtacagg gtgttcatca gttgggaaat
     8521 cctggcgcgc attcctcgct caccattcga aagggaggga gcttgggaca gcagacgagc
     8581 caggtcgcga tgcgccgatc gcgcgtttgg gtagcgatct ttcgtatgaa acgccgtgag
     8641 tttggcgagg atgtcatcga gttccgccgg tgcgttgacc aacgatgcca gtttcggcaa
     8701 aggacattga ttttttgcga ctttcgccag atggtccgaa gcttcccctt gcaagaaccg
     8761 ccgtcctgcc aatagctccc acaaaatgac gcccaaggcg tagatgtcga ccagcgcatt
     8821 gcccgtattg ccattggcca cttcaggcgc cacgtagccg ggcttggcaa aaacgacacc
     8881 cgccaccgta tggctgcgac gattttcccc gagcgccgta ccaaagtcga tcagttttac
     8941 gtccccgcca tagccaacca tgatgttttg gggactcata tcccgatgca cgatgctcaa
     9001 cggctggccg ctcacgtcga ctcgctcgtg gatatgggcc agcgcttcgg cggtcgatgc
     9061 cgcgagcgcc accgcttccg cccaactcag ccgataggaa acgtccgcgc aacgagaacg
     9121 gacttctcca aggctccggc cttcgacata ctccacgaca acatacggtt ccccttgctc
     9181 atcggtatca gcctcgatga cctgcacgac gccgggatga tgaagttggg cctgcacgcg
     9241 ggcttcatcg agaaagcgcg cgacaaaaga gctgttgctg gcatgttccc gtcgaatgat
     9301 tttgacgaca cagggacgct ccgcgccttc gatgcccgtg gttgcggcga gatatacttc
     9361 gcccatgccc ccgcgggcga tgagacgaag aagcgaatat cgtccgatac gacggcccag
     9421 cgcggcgggc ggcaggtttt ttttcccggg gggcggtgct ttgggacggg gcgttggcgg
     9481 catgggaggc atggtgccta tgtaggatga agtggtcaaa ttattgaccg aggatatcga
     9541 cggaaaaccg agctttgggc cccgtgaaga agtctacagg cctgcattca taaggggatg
     9601 gagttgcagt tccttaggcc ccggccgggg agcaggtagt catggcggtg atgcaagatt
     9661 gcctgggcaa cgggtaggaa gacacgagcc attccaagcg aatactcgtt gtgtattgtg
     9721 taatatattg aatttatgat gtattttcga aagcccctcg gtcgcggtag tggtggcggt
     9781 tgctgcggta ggctcacgca tctaggttgc cttgtggcaa cgtctgagta gacttccgcc
     9841 gttcgtcgcc atggccttgt cgatatttcg caccactatc gttttcgcca cgttgctggt
     9901 ggtgttggga tgggtgcggg tggcgactgc ctatgcccct ttctgtgatc cacgtgcggc
     9961 aacgggtgtg gcgtttccgc ccttcacggt cattccggat ttacgctggg agccgtcgtt
    10021 tgaagtgttt tttctgcaaa gctgcgaacg aatgccggcc taccgcagcg tggagggaat
    10081 tccgcacccc gccaaagata agatgagcga aatgcaatcc ggccatggga tggaccttgc
    10141 ggctctgtcc aactccgtgg atattccgcg ccgttttcgt ccgacccggg ttgccctgtc
    10201 ggggcaacgg actccgaact tgcaggaaat attgcatcgg gtgtatcggc cgccgaaaca
    10261 cctatgattc attcggcaat gtgtagaaaa gtaggatcgt ttgattgatg aggtaataga
    10321 acatgaacaa aggaacagcc attgttggct tcatcctctc tttcctggcg gggatgatgc
    10381 tgatgtgggg gataaatcaa agttcgggac gcacaggaag cgagagcatc aaggcagaca
    10441 aggccggaaa atggagcgat gaggattcgc ccatttcggt gacgagtgaa gatccggtgt
    10501 ggggcaatcg caatgctccg gtcaccatgg tgattttcag tgaattcgag tgtcccttct
    10561 gcgcgcgcgt ggaaggaagc attgaagcca tcaagcagca atacggcccc gacaaggttc
    10621 gagtcgtatg gaagagcaat cctcttgcgt tccacaaaag cgcacgtccc gctagtgtag
    10681 ccgcggagac cgtccgcgcg ctggcgggca acgaggcctt ctggaagttt cataaaagcg
    10741 cgtttgccaa ccaaaaagcg ctgacccgag agaacttcga gaagtgggcg caagaagctg
    10801 gcgtcgacat ggcgaagttc aaagacgctc tcgacaagga gacgtatgct gcgaaagtcg
    10861 acaaggatct ggcgcttgga cagaagcttg gcgtgcgtgg aacgccgcat actttcgtca
    10921 atggcgtgac ggtcaatggc gcgcagcccg tggataagtt caaggaagtc gtcgatgcgc
    10981 aactcgcggc ggctcaggcg atgatcaagg cggggacgaa gccggacaag gtatacgtgg
    11041 aagcctcgac caagaacttc cagaagcctg cggagcagcc gaaaaacgag cgagaagccc
    11101 cggcggaaga caagacggtg tggaaggtga cgatcgacaa gtctccttcg aaagggccca
    11161 cggatgcgct gttgactctc gtgattttca gcgagtacca atgcccgttt tgcaagcgtg
    11221 tggagccgac catcgaccaa ctcatgaagg attacgatgg aaagttgcgc gtcgtatgga
    11281 aggaccgccc ccttcctttc cacaaggaag cggagcctgc ggctctgttt gccctggaag
    11341 cgcgtaagca gaagggtgac aagggttttt gggatgccca caatctgctg ttcgagaatc
    11401 agcccaagtt ccagatggag gacttcgagc aatacgccaa gaccctcggc ttgaattgga
    11461 cggcggtgaa aaacgcgatc gagaaaaaga cccacgcggc ctcgatcgaa acagacgtgg
    11521 ccttggccga tggcctgaaa gccagtggaa ccccgcatat gttcatcaac ggacgtcgta
    11581 tcgtgggggc tcagcccatc gatgctttca agaagttgat ggatgaggag ctggcgaaag
    11641 ccgaagcgat ggtcaagggt gggacagcga aggcttctgt gtacgagacg atcatgaaga
    11701 ctgcgcagac gcctcctcct ccgccgccgc ccgagaaaaa ggacgtgcct gcgccgaccg
    11761 cgcagaaccc ctataaaggc ggaaaaaacg cgaagatcgt cattcagctc ttcagtgatt
    11821 tcgagtgccc gttctgcggt cgtgtggaac ccgtgatgga agagatcctc gcgaagtacg
    11881 gcgacaaggt gaaagtggta tggcgccaat tcccgcttgc cttccataaa aatgcaaaac
    11941 tcgcggcaga ggcttcggtc gaggtattca agcagaaggg tgatgcggcg ttctggaaat
    12001 accacaacac attgttccag aaccaaaagg cgctcgagcg cgcggatctg gagaagtacg
    12061 cggaagaact tgggtgcgat atggcgaaat tccgagctgc tctcgacaac cacacgcacg
    12121 cgaagttcgt ggaagacgag attgcggttg cggaaaaggc cggaattcga ggcacacccg
    12181 cttcggtcat caatgggtat ttcgtaagcg gtgcccagcc tttcgctcag ttcgagaaga
    12241 tcctcaagct cgctgaatcc gaactcaaat aatccccccg cccgttagtc atcaggtatc
    12301 cctgccacgg tgccttcctc gttctggagg cgcccacgct cgccacgcac gaatcgttca
    12361 ttgtttcgtt cgttccgaga tcgccttgat ggtgtcattg agcttccgat cggcccgcat
    12421 gcacttccaa caatcactga gaccaagaaa accgcatccc ttggcgtgta gaagccctcg
    12481 aagttgcgtc aacgaccgct cgtcgccgtg ttcccgggcg cggtcgagga gtctgtgttt
    12541 ggcctgacaa ccgccattga aacgcaaatc caacgcgatg agcagtgcag gggaagcgtt
    12601 tttgcgtgcc gcctcggact tgatggactt ggcggcgcga gaggcaatcg cggctgtggt
    12661 cgtacgtttc ccaaacgcca agtcgtggat cagatcgggc ccctccgttc cccaatggct
    12721 ttccaacaac gaaaaaacaa cctcttgaga ggacggcttg gtcaatgcgc ccagtacgtc
    12781 ttccagcacg gcgttgtctt ttcgtttttc cggatcgagg gaaaagagct tgtcatatac
    12841 ctcgatgcat gtgtcggggg acgcgccccc tagccttgct ttggccactt gcgcgagcac
    12901 ggcgggatcg ttggggaatt gcgtgagcag ttgttcgatc gattcgctcc ccgctttcac
    12961 tgctttttcg agttgttggg gatcggcagt ggctgaagcg ttttgcatcg acgacgcgac
    13021 gggaggggaa gcccaagagg tttcatcaaa aacgggtttg accgtggaag gggtgcgaac
    13081 ccaccagacg attccgatca cgacgatgag tgcgagcatg ccgacccccg cggcaaggcc
    13141 gagccatacc gacagcttga gggtacgcag cggagccggt aatgcggctt gccatcgatg
    13201 gggttgagcc tgggcgtccg cggcgcgaag cgtttgtgcg agttgtgatg accatgcgga
    13261 aattctcgcc atcacacttg cgatggacgg agcgcgtggt ttggcggacg ctattccttc
    13321 gaacacggtg ggggcatgcg tgaggctcat ggcagcggac acattggatc tggcatcctc
    13381 agtccaggac gacgtgtcga ggttttgctc gagaatttcg aagacgtcgc gcacctgttt
    13441 ggcatcggac ggcctgcgcg cggcttcttt ttcgagcaat tccatgacaa tcgcctcgat
    13501 ctcctgcggc aggtgcaggc cgggagccac ttggcgaaag gcaggcggtg gctgcgtgac
    13561 ttgcattcca agcaaggcca cgggattgtc cgcttcgaaa ggtcgcctgc ccacgagcat
    13621 ttcgtaaaga atgacgccaa gagcgtacag gtccgcgcga gcatcgacgt cctgtcccag
    13681 cgcttgctcc ggagccatat actccggcgt gccatagacc attcctactt tggtcagtgt
    13741 tttatgctgt tttccagctt ctttgctgat ctgagagaca ggcaccttcg cgatgccaaa
    13801 gtcgagcacc ttgacgaaat cagggtctgt atcccgagaa acgagcatga tgttttcggg
    13861 tttcaggtct cggtggacga tgcccagacc gtgggcgcga gcgagcgcgg tcgcgatctg
    13921 ttttccgata tgaatgacgc gacgggggtg catgggaccc tgctgcaaga c
//
LOCUS       DAOSOR010000077        13931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4418_length_13931_cov_4.310248, whole genome shotgun sequence.
ACCESSION   DAOSOR010000077 DAOSOR010000000
VERSION     DAOSOR010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 13931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13931
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4418_length_13931_cov_4.310248"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..683
                     /locus_tag="PK710_06145"
     CDS             93..683
                     /locus_tag="PK710_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOT09330.1"
                     /translation="MRRLSLALVHHPVLDRQGDVITTAITNMDLHDMARSGRTYGASD
                     LFVVHPVAAQRQLAERIQHHWLYGSGQRRIPDRAPPMALLRIVSSLEEAFEAMGGRDN
                     LEVWVTSARDVGASCSTGQARQRLWQQGPPVMLLLGTGWGLAKNLINSADLCLEPLRA
                     ATETGFNHLSVRAACAILLDRLMGQRMDDADRLSTT"
     gene            complement(695..1405)
                     /locus_tag="PK710_06150"
     CDS             complement(695..1405)
                     /locus_tag="PK710_06150"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOT09331.1"
                     /translation="MMDGSTTIDGFFDGALWLEQPAPGFGYRANEDSLLLASFASRCR
                     PAKTTLIDLGAGVGTVALALHLIHPQTQLTLVEQQPFLADLARNNIVRARLEHVTNVV
                     QAEVALWSDDQPAFDGMVVANPPYTPPHSGRRAIEPSRDAARHGELTPFLHAMGRLIA
                     SSQGTACLCYPVSDLLNLITTASRNGLYPVRLAFVQRNQRQDTRLALLELRGRPNTPL
                     VVEPPIVGPFSFPGSPNE"
     gene            1488..2429
                     /gene="queG"
                     /locus_tag="PK710_06155"
     CDS             1488..2429
                     /gene="queG"
                     /locus_tag="PK710_06155"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOT09332.1"
                     /translation="MNDRSEEVRRMALHLGFDAVGIARADEPLEVDYARYEEFIDAGY
                     QATMDYLAAHASVRRVLSHPGILPGARSVVCVLQRYAVRLPSASTGIVGHIAKYARGL
                     DYHNHMRRRLRKLADFIRKMESGATSRPLVDTAPVLERAWAARAGLGFIGKNGLLIRP
                     GMGSFTVLGEVVTSVVLEPDEPMPSRCGECVACLQACPTEAFVGPYVLDARKCISYLT
                     IEHRGPWQGEPKVAPWLFGCDVCQDVCPFNRGVGAWIDAGGPFDPRPQWHQLSLEQLA
                     CAAQSEGQIWMQASPMKRVRWEDWQRNVRGALGYDPP"
     gene            2715..2978
                     /locus_tag="PK710_06160"
     CDS             2715..2978
                     /locus_tag="PK710_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09333.1"
                     /translation="MSYWLEMQEGDWARSPHSRARYALYRGLTHLSLGDEESAQRWLA
                     EAKDVTDRQQGVLDDEDMGRLLVAWRVIGHEPGTWGRDVLESR"
     gene            3032..3751
                     /locus_tag="PK710_06165"
     CDS             3032..3751
                     /locus_tag="PK710_06165"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOT09334.1"
                     /translation="MNEFSSNPSTRIRRTAQYGPVTIALSVVFGVVFLVESYVDGGGL
                     WQISASTLGKLGGNAPVLTFGEGQWWRLVTAALMHGGLLHIAFNTYANLIIGSAIEKT
                     LGPGWVLGPFVFSAITGSLLSAYANPANVVAIGASGGIFGLVAMAAAISYLAPRLVGF
                     SRSVLVQWLVFALAIGVVAGFDNWGHVGGIVGGAICALVVALFKRQPVWVRKVGWVGA
                     ALGALVMAGSVAMAAQHYLAL"
     gene            complement(3762..4604)
                     /locus_tag="PK710_06170"
     CDS             complement(3762..4604)
                     /locus_tag="PK710_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09335.1"
                     /translation="MSPAIRVNLCQPIDESHALRDAESASLSHPYGSAGLLACDSLKS
                     CLMNRRAHAATMLALILMASPAEAQSQPASPFDANGPLAPWKGPLPSEPHPLAVIARA
                     TPDQPPTPQDEPHTTIAVCINPALTVLGKTSARLELAPLGSHALFVEMSRLSFFISEL
                     NRKVTGTEYDIGYHLFPQGQGADGFYIGPRFLFGSGEITEARWSFRGFGGDLGYQWVI
                     AHHLVFNLGGGVARIQGKAELEQDALGLPKQIPENVSGAFSLSSQQASLWVPMLTLAL
                     GFAL"
     gene            complement(4632..4829)
                     /gene="rpmF"
                     /locus_tag="PK710_06175"
     CDS             complement(4632..4829)
                     /gene="rpmF"
                     /locus_tag="PK710_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOT09336.1"
                     /translation="MAVPKRRTSASKRDMRRAANEKIVAPNVIACPNCNEPTLSHRVC
                     PNCGHYKGREVIVMKADEAEE"
     gene            complement(4840..5445)
                     /locus_tag="PK710_06180"
     CDS             complement(4840..5445)
                     /locus_tag="PK710_06180"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOT09337.1"
                     /translation="MAHRFVFSIKDLESGVHDVQEPLPCPWLQEILDDTDVTVPPSPE
                     GLLEASLTLTGRNVLVRGHVQATVSLACARCLQPATIPIEAELSLLLVPGKPQKPKPA
                     TSKAFSNQRRHAGAMEDDDDGDAIGPNDAELDTYTGDEIVLDGFIREAILLELPIFPL
                     CSEACPGIGALPSDSAQSATEGPPIDPRLAPLLELKIKSKA"
     gene            5549..5827
                     /locus_tag="PK710_06185"
     CDS             5549..5827
                     /locus_tag="PK710_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09338.1"
                     /translation="MQKKALSIIVLALCVVFTLATVVNVFGDNQDVIEKAEKAVCWNV
                     SQCKYAKTSMIRTPIGQTFTFEASGKSTEVVCRRAFIILGEYSCEVEK"
     gene            5971..7338
                     /locus_tag="PK710_06190"
     CDS             5971..7338
                     /locus_tag="PK710_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09339.1"
                     /translation="MRSTSVGVWRGWFAKDSTKRRLLLGVMIYVLCTAAFFATTARER
                     IFEHTPYNHYALLADSWLHGQLDMRGPPPPYAGGNDFAQKDGKWYVSFPPFPAVWLLP
                     WVKLGGVVENVRDGQAFLWLAGVGPAGLWLVLERLRQRKLSERREGTNFVLALLFALG
                     TVYWFTAVQGTVWFAAHVVAVAVGAFYLLSALEARHPLWAGLLLGCAFLTRPPMLLMG
                     LFFAAEAFRMSMGHNEGKTRGGKLALIHQTLRSLAWKSFLKRCALFALPLVAVLALSL
                     WHNHARFGSPLEFGHEYLTIAWRSRIEKWGLFSYHYLPRNLSVLLASLPYLNQVPSGV
                     QINVHGLALWFTTPMYLWLLWPKKNTWLVIVLSLCAAGIAFMDLLYQNTGWMQFGYRF
                     SNDYALLLFVLLALGDRRFGTLFWSAAAFSVAVNAFGAMTFDRGPYKKFYHIDGSQKT
                     LFQPD"
     gene            complement(7396..9189)
                     /gene="hflX"
                     /locus_tag="PK710_06195"
     CDS             complement(7396..9189)
                     /gene="hflX"
                     /locus_tag="PK710_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOT09340.1"
                     /translation="MTQLFGNTSGLSPLATQKLERIYRRRVPLDTIATPEMVRSLCEA
                     SHEASRQVGALVHRSGQVDYVIVGDSNRLVLPDFGRLRATSGRFRGLRLVHTHLHGEP
                     LSHDDLVDLVRLRLDMVVALQLNPAGEPRAVSYAHAIPNQSHGPPYLIIGPDHPSRLR
                     DDFGNLISNLEREFARHTNSHALVAQTERAILVQVGSKADRDGFQRMEVSLRELRELA
                     RTAGVEIVDTLSQLRDRIDPKFVMGKGKLEELIVRAIGTNVDLVIFDNNLTPTQASNI
                     ARHTDLRVIDRTQLILDIFAQRAQSRDGKLQVELAQLKYALPRLSQKDDSLSRLTGGI
                     GGRRGPGETKLEIGRRRARERVTRLEQELKNLERQRRQRRRKRNRLDVPILAIVGYTN
                     AGKSTLLNTLTHAQELAEDKLFATLDPRSRRLRFPEDREVVLTDTVGLIRDMPEDLFA
                     AFRATFEEAADADLILQVIDASDQDRDQHVRTTEKMLADIGCEQIPRIMVFNKADLLD
                     PGEGHRLANTMDNAVVVSALHRETTRALLDMIGEKLADRWNTSACLPPPQIVSSPHVD
                     DETIEDPEPSLTTFEQLRQASGRRSRRTNAA"
     gene            9383..10273
                     /locus_tag="PK710_06200"
     CDS             9383..10273
                     /locus_tag="PK710_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOT09341.1"
                     /translation="MARVGQLDFQGWLVDRQASASTGEDGGEHAYAYISDRQTRAAFQ
                     RLVPVQLAVEAVVRMFKAFGKNELLGTSVRVGPRQFPRVHGIAQSCARSLGIATPTVY
                     IANNPTLNAGTFGTNDDSFILVHSALVDHMSDEELTSVLGHECGHIHNSHVVYLTALH
                     FLTRMAGRFVRGIVFPAFLALKAWSRRAEITCDRASLLCVHKPEVSTRALTKLALGSQ
                     KLYEELNLDAFLEQGEESRQGPGRYNEAFASHPWVPKRVLALRVFEQSRLYRRRAGLG
                     DDGLSMTEVDERVHEIVKVM"
     gene            10273..12048
                     /locus_tag="PK710_06205"
     CDS             10273..12048
                     /locus_tag="PK710_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOT09342.1"
                     /translation="MLDAFLEKRNVVLEVLNRLLTMADELGSSSSRDRIHKHIIEKLE
                     KNIFHLVIVGEFNHGKTTFVNALLGKQVFAVGVTPTTAVIHEIGYGKEPWAQVTYESG
                     MRQELRFDEVKYFAIGHPAPVPDPGPVRLLEIRYPAEILRNHVALVDTPGVNDLSLAR
                     AEITYDYIPRSDAVLFLLDAGQLIKESERVFLQDKLLGQSRDKIVFVVTKKDILDSQD
                     LEQAMGYVNVQLGKLIPNPRVHFVSAEKALEGAREESGMDELLRFLTTFLSEERGRIM
                     LDNALGDGIKACGLLSKGIDAKKRALEMNVEEIDRRLAAIQEDLAGQAKTIEQRRAMI
                     REEVAAIKAWTRRDLEGFVRDVCDQIPAIVDNARMDELKAHLGSFLEKTLRQWARRQT
                     EEIAEALEKLAEKTIALVREDARDAAKRIASKMGADMHSPTIEVNTFAYDVGVAALAG
                     VGVAFMFSNWMLGGVLLLAAPALALFMRGYVETETRNKAKQLAPQAVREAAAKVGPKL
                     EEMIDAFASNLDAWVINAGEEVHREVLDVLTAARRERERGEAARALAASDCQKHEGQL
                     QQLRQHLESLRAGLRGDAQESGPSQ"
     gene            12245..12457
                     /locus_tag="PK710_06210"
     CDS             12245..12457
                     /locus_tag="PK710_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOT09343.1"
                     /translation="MTVGKVKWFNDAKGWGFIKQETGPDVFVHYSQITGDGHRSLAEN
                     EMVEFEIQEGPKGLLAVNVTRSAQAH"
     gene            complement(12714..12896)
                     /locus_tag="PK710_06215"
     CDS             complement(12714..12896)
                     /locus_tag="PK710_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09344.1"
                     /translation="MNNAGGSVHGFPQVMAMVPVWRALCVEAAKQTSSYVPLSSLRIR
                     RRPQVYANVSHPASYG"
     gene            complement(13254..>13931)
                     /locus_tag="PK710_06220"
     CDS             complement(13254..>13931)
                     /locus_tag="PK710_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09345.1"
                     /translation="ATANCNDVCETYSELPNAEDHLNCVIGCNDNTCMKTCDQTYPAE
                     GAALENYFDCLGDKCAVQCPGFGPAYQICDSGLQMSNQSCATCLGDNCCSQIKACEAN
                     QNCLACITGTGGTNCNQDLLLTAVSTCWEQHCDAACKTTGQCTIGFTDPSMAACNDCM
                     KAKCEQECIDASDADGITAYLDCAGNCTTPACMDSCGTQYPAAAAAIGVLDDCLATKC
                     PTECNAP"
BASE COUNT         2865 a         3438 c         4436 g         3192 t
ORIGIN      
        1 aaagagcgtg cgtgggagcg gacaaaaaac aagcggcctg atcttcttgc tctcgatgat
       61 gctgcaacga caaagaaaga gccaacgtga cgatgagaag actcagtttg gctctcgtgc
      121 atcatcccgt gttggatcgt caaggggacg tgattaccac cgcgatcacg aacatggacc
      181 tgcatgacat ggcgcgcagt gggcggacct atggagccag cgatctgttc gtggttcatc
      241 ccgtggccgc gcaacgacag ctcgccgaac ggatccaaca tcactggcta tacggatccg
      301 gacagcgtcg cattcctgat cgagcgccgc cgatggcctt gttgcgaatc gtctcgagtt
      361 tggaagaggc cttcgaagcg atgggtgggc gagacaacct cgaagtatgg gtcacgagtg
      421 ctcgtgacgt cggagcttct tgttccacgg ggcaagcgcg gcaaaggctg tggcaacagg
      481 gcccgcctgt gatgctgctg ttggggaccg ggtggggact ggcaaaaaac ttgataaatt
      541 ccgctgactt atgccttgaa ccgctccgtg ccgcgaccga gacggggttc aatcatttga
      601 gtgtacgagc ggcttgcgcc atcttgctcg accggttgat ggggcagcgc atggacgatg
      661 ccgatcggtt gtcgacgaca tgagtgtctg agctttactc gttgggggac cctgggaacg
      721 aaaaggggcc aacgatgggc ggctcgacga ccaggggggt gttggggcga ccgcggagtt
      781 ccagcaaagc gagtcgtgtg tcttgtcgtt ggtttcgttg tacgaaggcg agccggacag
      841 ggtacaaacc gtttcgtgat gcggtggtga tcaggttgag caagtcagaa acggggtagc
      901 acaggcatgc tgtgccttga ctgcttgcga tgagtcgtcc catggcgtga aggaatggag
      961 tcagttcacc gtgcctcgcc gcatctcggg atggttcgat agcccttctt ccgctgtggg
     1021 gaggtgtgta gggcggattg gcgacgacca tgccatcgaa ggcaggttga tcatccgacc
     1081 ataaggcgac ctccgcttgg accacgttcg tcacgtgttc gagtcttgcg cgaacgatgt
     1141 tgtttcttgc gaggtcggcg agaaacggtt gttgttccac gagtgtgagt tgggtttggg
     1201 ggtggatgag atgaagggca agagcgacgg tccccacgcc ggcccccaga tcgatgagcg
     1261 ttgttttggc tgggcggcag cgtgaggcaa atgaggcaag aagcaggctg tcctcattgg
     1321 ctcgataccc aaaccctgga gctggctgtt cgagccacaa agcaccgtcg aagaacccat
     1381 cgatcgtggt ggagccatcc atcatcggac ctgtgagaag atgcgtgcaa accgagccaa
     1441 tggtagtgtg aatgccatca tggggtagaa cgtgacacta ggtcgagatg aacgatagga
     1501 gcgaagaagt acgccggatg gcgctccatc ttggcttcga tgccgtggga attgcacgag
     1561 ccgacgagcc gttggaggtg gactatgctc gctacgaaga attcatcgat gcgggctatc
     1621 aagcgaccat ggactatctg gcagcgcatg cttccgtgcg tcgagtgctt tcgcacccgg
     1681 gcatattacc cggagcgcgt agtgttgtgt gtgtccttca gcggtatgcg gtgaggcttc
     1741 catcggcgtc gacggggatc gtgggacata ttgccaagta tgcgaggggc ctcgattatc
     1801 acaatcacat gcgccgtcga ttgcgcaagc tagcggattt tatacgtaaa atggaatctg
     1861 gggcaacctc gcggccgcta gtggatacgg cacccgtgtt ggaaagggca tgggcagccc
     1921 gggcgggtct tggtttcatt ggaaaaaatg ggctgttgat tcgtccggga atgggttcgt
     1981 tcaccgtgtt gggcgaagtc gtgacctcgg tggtgttgga gcccgatgaa cccatgccat
     2041 cgcgttgtgg tgagtgtgtg gcttgtttgc aagcgtgtcc cacggaggct ttcgtgggcc
     2101 cgtacgtgct cgatgctaga aagtgcatca gttacttgac gatcgagcat cgggggccgt
     2161 ggcaagggga gcccaaggta gccccatggt tgttcgggtg tgacgtttgt caggacgtat
     2221 gtccgttcaa ccgaggggtt ggagcatgga ttgatgcggg aggtcctttc gatccacgac
     2281 cccaatggca tcaattgtcc ctcgagcagt tggcctgcgc agcacaaagc gaagggcaga
     2341 tatggatgca ggcaagtcca atgaagcgtg ttcgttggga ggactggcag cgaaacgtgc
     2401 gaggggcgct tggctacgat cctccttgag cgatcctcat cacgatcggg atgacctcga
     2461 tggcgttcgg tgcggtgggt cgatgcggtg ggtcgatgcg gtgggtcgat gcggtgctgg
     2521 gcgcgggctt tgatatgccc gtatgaagca attttttatg gtttgacgaa cacgcacaac
     2581 cgatcgcgac cggtgtccga agaaaggata tgcgttgcgg aaactgggtt ttggtttcgt
     2641 ttttgttttt gctccattgc tcgacgatgt cgcagggggt gcgggcttat cactctgggc
     2701 tacacacgcg tgcgatgtcc tattggctcg agatgcagga aggcgattgg gcaaggagtc
     2761 cccattcgcg agcgcgatat gccctgtatc gtgggctaac ccatctttcg ttgggagatg
     2821 aggaatcggc gcaacggtgg ttggctgaag cgaaagacgt tacggatcga cagcagggtg
     2881 tattggacga tgaagacatg ggtcgattgc tggtagcgtg gcgtgtgatt ggccatgaac
     2941 cgggcacgtg ggggcgcgat gtgttggaaa gccgttgatc ggaaaggctc ttgctttctg
     3001 ggcggttcag ttccaggttg gccctcgtga tatgaacgaa ttctcatcga atccgtcgac
     3061 tcgtattcgc cggacagcgc agtacggtcc ggtcaccatc gcgctcagtg tggtgtttgg
     3121 tgtcgtattt cttgtcgaat cgtatgtgga tggcggaggg ctttggcaga tttccgcgtc
     3181 tactttggga aaactcggag gcaatgcacc ggtgttgacg tttggggaag gtcagtggtg
     3241 gcgcttggtc actgcggctt tgatgcacgg agggttgctt cacatcgcgt tcaacacgta
     3301 tgcgaatctc atcattggtt ccgcgatcga aaagactttg ggtcctggtt gggtgcttgg
     3361 tcccttcgtg ttttcggcga tcacggggag ccttctttcc gcctatgcga acccagccaa
     3421 cgttgtggcg attggagcat cgggtggcat tttcggattg gtcgcaatgg cagcggccat
     3481 tagctatcta gcaccgcgac tcgtgggttt ttcacgaagc gtgctcgtgc agtggttggt
     3541 gttcgccctg gcgataggcg tggtggcggg attcgacaat tggggtcatg tcggtggaat
     3601 cgtgggagga gcgatctgcg ctttggtagt agccttattc aaacggcagc ctgtatgggt
     3661 tcgaaaggtc ggttgggtcg gcgcggcatt gggagcgctt gtgatggcag gcagtgtagc
     3721 catggcggca cagcattatt tggcgttgtg atggttcggc ttcagagggc aaatcctagg
     3781 gccagcgtca gcatgggaac ccaaagcgag gcttgttgag aagaaaggga gaaggcgccg
     3841 gagacgtttt cggggatttg tttgggaagg ccgagagcgt cttgttcgag ttccgctttc
     3901 ccttggattc tcgccacgcc gcctccgaga ttgaagacga ggtgatgggc aatgacccat
     3961 tgatatccga ggtcgccgcc aaagcctcga aagctccacc gggcttccgt gatctcgcca
     4021 cttccgaaaa gaaatcgagg tccgatatag aagccatccg ccccttgtcc ctgcgggaag
     4081 agatggtagc cgatgtcgta ttccgttccg gtgactttgc gattcagttc actgatgaaa
     4141 aacgacaggc ggctcatttc gacaaataat gcatgactac ccaagggggc cagctccagt
     4201 cttgcactcg tttttccaag gacagtcagc gcaggattga tgcagaccgc gatcgtcgta
     4261 tggggttcgt cttggggcgt gggtggctga tcaggcgtgg cgcgggcgat aaccgcaagc
     4321 ggatgaggtt cggaagggag cgggcctttc cacggggcta acggaccgtt ggcgtcgaaa
     4381 ggggatgcgg gctgggattg tgcctccgca ggggaggcca tgaggatcaa tgccagcatc
     4441 gtagccgcgt gagcccgtcg gttcatgaga caggatttga gcgaatcgca tgccaacagc
     4501 ccagccgagc cgtagggatg agaaagcgaa gcactttcag catcacgaag cgcgtgggat
     4561 tcgtcgatgg gctggcacag attgacccga atcgctgggc tcatgcgcca cgttggtaga
     4621 aaagaagcga actattcttc ggcttcatcc gctttcatca cgatgacttc acgccctttg
     4681 tagtggccgc aattggggca tacgcggtgg gaaagggttg gttcgttaca attggggcac
     4741 gcaataacgt tgggagcgac gatcttctcg ttcgctgccc tgcgcatatc gcgtttcgaa
     4801 gcgctggtac gtcgcttggg gactgccacg gcgaactcct taggctttgc tcttgatttt
     4861 aagctccaac aaaggagcca atctgggatc tatcggtggt ccctcggttg ccgactgcgc
     4921 ggaatcggag ggaagcgcac cgatacccgg acatgcctcg gaacacaagg ggaagatggg
     4981 caattcgagc agaatcgctt cacgaatgaa tccgtcgaga acgatttcgt ctccagtgta
     5041 agtgtcgagt tccgcgtcgt tggggccaat ggcatcgcca tcgtcatcgt cttccatggc
     5101 gccagcatgg cgtcgctgat ttgaaaaagc cttggaagtc gcgggcttag gcttttgtgg
     5161 cttgccgggg acgagcagca gggacaattc tgcctcaatc ggaatggtgg ccggttgaag
     5221 acaacgagcg caggcaagcg atacggtggc ttgcacatgg ccccggacga gaacattacg
     5281 accggtgagg gtgagcgacg cctcgagcaa gccctctggt gacggcggaa cggtcacgtc
     5341 ggtgtcgtcg aggatctctt ggagccaagg gcagggcagc ggctcttgga cgtcatgcac
     5401 tccactttcg aggtccttga tggaaaaaac aaaccgatgt gccatgaggg gctgcaaagc
     5461 atagcggcag gaaggtgagt cggcaatcgc ggaaacgtct catcattggc tcgtgtctcc
     5521 ggctcgtgtc tccgctacac tatcgctcgt gcaaaagaag gccctaagca tcattgttct
     5581 agccttgtgc gtggtgttca cccttgcgac ggtggtcaac gtatttggtg acaatcagga
     5641 tgtgatcgaa aaggctgaga aagcggtttg ctggaatgtg tctcagtgca agtacgcaaa
     5701 aacctcgatg atacgcacac cgatcggcca gaccttcacg ttcgaagcgt cgggcaagtc
     5761 cacggaagtc gtttgccgtc gtgcttttat catcctcggt gaatactctt gcgaagtgga
     5821 aaagtagagg cgcgttgtcg tcatcgacag cgtccttggt ttgccctgag ccatcggctg
     5881 gccgaggtgt tcggggcttg cggttccatg ggaaggctct gattgtacga tggtgaggat
     5941 tgtgtgacgg ttttggctga atcaaagccc atgcggtcga cttcggttgg cgtatggcga
     6001 ggatggtttg cgaaggacag caccaagcgg cgtttgctgc tgggggtgat gatatatgtt
     6061 ctttgcacgg cggcgttttt tgccacgacg gcacgggagc ggattttcga gcatacgcct
     6121 tacaaccact atgcgttgct tgcggattcg tggttgcacg gtcaattgga catgcgtgga
     6181 ccgccgccgc cttatgcggg aggcaacgat ttcgcacaaa aggatggcaa gtggtacgtg
     6241 tcgtttcctc catttcccgc cgtatggttg ttgccgtggg tcaaacttgg tggcgtggtg
     6301 gaaaacgttc gtgatggtca agctttcctg tggcttgcgg gagtgggtcc cgcgggtctt
     6361 tggctagtcc tcgaacgatt acgccagcgg aagctcagcg agcggcggga gggaacgaat
     6421 ttcgtacttg cgctgctgtt cgcgttagga acggtctatt ggttcacggc agtgcaggga
     6481 acggtatggt ttgcggccca tgtggtagcg gttgcggtag gcgcgtttta tttgctttcg
     6541 gcgctcgagg cgcggcaccc gctttgggct ggactgctgt tggggtgcgc ctttctcacg
     6601 cgtcctccca tgctgctgat gggactattt ttcgccgcgg aagcgtttcg aatgagcatg
     6661 ggtcacaacg agggaaagac gcgggggggc aagctagcgc tgatccatca aaccctgcga
     6721 tcgcttgctt ggaagtcgtt tttgaaacgg tgcgcccttt tcgcgctacc gcttgtggcc
     6781 gtattggctt tgtcgctttg gcataatcat gcgcgcttcg gaagccctct ggagtttggt
     6841 catgagtatc tgacgatcgc atggcgcagc cgaatcgaaa aatggggctt gttttcctat
     6901 cactatctgc cgcgcaatct gagtgtgctg ctcgccagtc ttccctatct caaccaagta
     6961 ccatcgggag tacaaatcaa cgtgcatggt ctcgccttgt ggttcaccac gccgatgtat
     7021 ctatggttgc tctggccgaa aaaaaatacc tggctcgtga ttgtcctttc tctttgcgcc
     7081 gcgggaatag ccttcatgga tctgctgtat caaaacaccg gatggatgca gtttggctat
     7141 cgtttttcga atgattacgc gctgttgctc ttcgtactcc tagcccttgg agaccgtcgt
     7201 ttcggcaccc tgttttggtc cgccgccgcg ttttccgtag ccgtcaacgc cttcggcgcg
     7261 atgaccttcg atcgtgggcc ctacaaaaaa ttctatcata tcgacgggtc ccaaaagacg
     7321 ttgttccagc cggattgatg gtcatccatc cgtgggattt tggggtaagc cgcatcggaa
     7381 cagacagagg attggttacg ccgcgttggt tcgtcgagac cgacgtcccg atgcctgtcg
     7441 tagctgttcg aacgtggtga gcgacggttc ggggtcttcg atggtctcat catcgacgtg
     7501 tggtgaggag acgatttgtg gtggtgggag gcaggcgctt gtgttccagc gatccgcgag
     7561 tttttctcca atcatgtcca acaacgctcg tgtggtttcc cgatggaggg cggaaaccac
     7621 gaccgcgtta tccatggtgt tcgcaagtcg atgtccttct cctgggtcaa gcagatccgc
     7681 tttgttgaac accatgatgc gggggatctg ttcgcaacca atatcggcga gcatcttttc
     7741 cgtggtgcgc acatgctgat cccgatcttg atcggaagca tcaatgactt gcaaaatgag
     7801 atccgcgtcg gcggcttctt cgaatgtcgc gcgaaacgcc gcaaatagat cctccggcat
     7861 atcgcggatc aagcctaccg tatcggtgag caccacttcc cggtcttcgg ggaaacgaag
     7921 acgacgagac cttggatcga gagtggcgaa cagcttgtcc tccgcaagct cctgcgcgtg
     7981 tgtcagcgta ttgagcaagg tgctttttcc cgcattggtg tagccaacga tggcaaggat
     8041 gggcacgtcg agacggtttc gcttacgtcg tctttgcctt cgttgacgtt cgaggttttt
     8101 gagttcttgc tccaatcgag tgacacgctc gcgagcacgg cgacgtccga tctcgagttt
     8161 ggtttctcca gggcctcgtc gtccgccgat gcccccagtg agacgagaaa gggaatcgtc
     8221 tttttgactt agacgaggca acgcatattt gagttgggcc aattccactt gcagtttgcc
     8281 gtcgcgactt tgtgctcgtt gcgcaaaaat atcgaggatg agttgtgttc ggtcgatgac
     8341 acgcaaatcc gtatgccgag cgatattcga tgcttgtgtg ggggtgagat tgttgtcgaa
     8401 gatgaccaga tccacattgg ttccgatggc gcgcacgatg agttcttcga gtttcccttt
     8461 ccccatgacg aacttggggt cgatacggtc gcgcagttgg ctcaacgtgt cgacgatctc
     8521 gacgcctgct gttcgcgcaa gttcacgcag ctctcgcaac gagacctcca tccgctgaaa
     8581 accgtcgcga tccgctttac ttcccacttg cacaaggatg gctcgttcgg tttgcgccac
     8641 cagggcatgg gagttggtgt gacgcgcaaa ctcgcgttcg agattcgaaa tgaggttacc
     8701 gaagtcatcg cgcaaacgtg agggatgatc cggtccgatg atgaggtagg gagggccgtg
     8761 agattgattc gggatggcat gcgcgtagga gacggctcgc ggttcacctg cggggttcag
     8821 ttgcagagcg acgaccatgt cgaggcgtaa tcgcaccaga tcgacgagat catcgtggct
     8881 caacggttcc ccatgcaagt gggtgtgtac gagtcgaaga ccccgaaaac gtccactagt
     8941 ggcccgaagc cgtccgaaat cgggcagcac caggcgattg gagtcaccca cgatgacgta
     9001 gtcgacttgc ccggagcgat gaacgagagc gcctacttgc cgggaggctt cgtgagaagc
     9061 ttcgcataag gaacgcacca tctccggggt cgcgatcgta tcgagaggga ctcgtcgtcg
     9121 gtagattcgt tcgagtttct gggttgcaag gggagaaaga cctgaagtgt tgccaaaaag
     9181 ctgcgtcatg ctgagatgcc aatacgtata tgcctcgccg ggttgtgaat cacagaaagg
     9241 agggagttgc gttcggaccc tcccggatgc aggaatcttc gcatgcgatt gccgattgtg
     9301 ccatgagcaa attgcgcaag ccgggggcaa acgctaccgt tgacaagctg cggtgtttga
     9361 gccgcgcagg aaggagctat cgatggcccg agtggggcaa ctggatttcc agggctggct
     9421 ggtggaccgc caagcttcgg catcgacagg ggaagacggt ggcgaacacg cctatgccta
     9481 catttccgac cgacaaaccc gggccgcgtt tcaacgcctc gtgcccgtgc aactcgcggt
     9541 ggaagccgtc gttcgtatgt tcaaagcctt cggaaaaaat gagctgctgg ggacctccgt
     9601 cagggtgggc ccccggcagt ttccacgagt tcatggaatt gcccagtctt gcgcgcgttc
     9661 gctggggatt gccacgccga cggtctatat cgccaacaat cccactctca acgcggggac
     9721 gtttgggacc aacgacgatt cgttcattct cgtacattca gcgcttgtgg atcatatgag
     9781 cgacgaggaa ttgacgagtg tgttgggtca tgagtgcggt catatccaca acagtcacgt
     9841 ggtgtacctg accgcgttgc atttcttgac ccggatggcg ggtcgatttg tgcgaggcat
     9901 tgtatttccc gccttcttgg ccctcaaagc gtggtcccga cgtgcggaga ttacttgtga
     9961 tcgcgcttcg ttgttatgtg tgcacaagcc ggaagtatcg actcgtgccc tgaccaaact
    10021 cgcccttggg tcgcaaaaac tctacgaaga actcaatctc gacgcgttct tggaacaggg
    10081 cgaggagagc cgtcagggac caggccgata caacgaggcg tttgcatccc acccctgggt
    10141 tcccaaaaga gtgcttgcgc ttcgggtttt cgaacagagc cgcttgtatc gtcgccgagc
    10201 cggattggga gacgatgggc tttcgatgac cgaggtcgat gagcgggttc atgaaattgt
    10261 gaaggtgatg tgatgctcga tgctttcctg gaaaaacgaa atgtcgtgct cgaagtcctg
    10321 aatcggctcc tgaccatggc cgatgagctt ggatcgtcgt cgagtcgcga tcgaattcac
    10381 aaacacatca ttgaaaaact ggagaagaac atatttcact tggtcatcgt gggtgagttc
    10441 aaccacggca agacgacgtt tgtgaacgct cttcttggca agcaggtgtt tgccgtgggg
    10501 gtaacgccga cgacagcggt cattcatgaa attggatatg gcaaagagcc ttgggcccaa
    10561 gtgacgtacg aaagcggtat gcgtcaggaa cttcggttcg atgaggtgaa gtactttgcc
    10621 attggccatc cggcgccggt tccggacccg gggcccgtac ggttgctcga gattcgctat
    10681 cccgcggaga ttctccgaaa tcatgtggcc ttggtggaca ctccgggggt caacgatctg
    10741 tcgttggctc gcgccgaaat cacgtacgat tacattcctc gatcggatgc ggtgctgttt
    10801 ttgctcgacg ccggtcaact catcaaggaa agcgaacgcg tttttttgca agacaaattg
    10861 cttggtcagt cacgagacaa gatcgtgttc gtggtgacca agaaagacat cctggattcg
    10921 caagacctcg agcaggccat ggggtacgtg aatgtgcagc ttggtaagct aatccccaat
    10981 ccgcgcgtgc attttgtcag cgcggaaaaa gctcttgagg gagcacgaga agaatcggga
    11041 atggatgagc tgttacgttt tctcaccacg tttctatccg aggaacgtgg tcgtatcatg
    11101 ctcgacaatg cgttggggga tggaattaaa gcatgcggac tgctttccaa gggaatcgat
    11161 gccaaaaaac gcgcgctgga aatgaatgtg gaggaaatcg atcgtcggtt ggcggccatt
    11221 caggaagacc tggcagggca ggcaaaaaca atcgaacagc ggcgagcgat gattcgagaa
    11281 gaggtggcag cgatcaaagc gtggacgaga cgagacctgg agggattcgt acgagacgtt
    11341 tgcgatcaga tcccagcgat agtggacaac gcgcgaatgg atgaattgaa agcgcatcta
    11401 ggttcgtttt tggaaaagac gttgaggcag tgggcccgtc gacaaacgga agagatcgcc
    11461 gaagccttgg aaaagcttgc ggaaaaaacg atcgcgctcg tacgggaaga tgcgcgagac
    11521 gcggcaaaac gaattgcatc gaaaatgggg gcggacatgc attcgcccac cattgaggtc
    11581 aatacgtttg cctatgacgt gggtgtggct gctctcgctg gcgtgggggt cgccttcatg
    11641 ttttcgaatt ggatgttagg tggggtgctg ttgcttgcgg ctcctgccct cgcattgttc
    11701 atgcgcgggt atgtggaaac ggaaacacga aacaaggcga agcagttggc gccgcaagcc
    11761 gtgcgagagg cagcggccaa agtagggccc aagttggaag agatgatcga tgcgttcgca
    11821 agcaatctcg atgcttgggt catcaacgcg ggggaagagg tccatcggga agtgctcgac
    11881 gttcttaccg cggctcgacg ggagcgagag cggggtgaag ccgcacgcgc gctagcagct
    11941 tccgattgcc aaaaacacga aggacaactt caacaattgc ggcaacacct cgaatcgctc
    12001 cgcgctgggc tgcgagggga tgcgcaagaa tcaggaccgt cgcagtaaac aaacttggat
    12061 ttgccgacaa tgacacgatt tttgcgcggc gcgagcgaat cttgtcactt tcctaccgca
    12121 tggcttgacc ccaacatgcc gttttcgata gcgtaagact gctccggaga ggacgcttcg
    12181 agccgttcga accggggggc agtcgttcgc gaagacgtcg cgagccagca agtggaggtt
    12241 tcacatgact gttggcaagg tgaagtggtt caacgacgcg aaagggtggg ggttcatcaa
    12301 gcaagagact ggccctgacg tattcgtgca ttattcgcag attaccggcg atgggcacag
    12361 gagtttggca gaaaacgaga tggtggaatt cgaaatccaa gagggaccca aggggcttct
    12421 cgccgtcaac gtgacacgct cggcacaagc ccactgaacc tcgcgattca cgcacctgtc
    12481 ttcttatcgg tcgtccaagt tctacgcgac aacctcatga gaagaagttc ccctcgccgc
    12541 tgttcgcgcc aaggggttcg tccgttttct atctagcctt cgcatggagc atcgtgggtg
    12601 tttttgcatg atgcctgcag ccttggacac agaggcgatg tcatctgttt tgtactgtcg
    12661 tttcaggtgt aactaattga atttgcagta atatttattg gacggacacg gggtcatccg
    12721 tagctcgcgg gatgggaaac gttcgcatac acctgcgggc gtcgccgaat tcgtagcgag
    12781 gatagcggga cgtacgagct ggtttgcttg gcagcctcga cgcacaaggc acgccagaca
    12841 ggtaccatcg ccatgacttg cgggaaacca tgcacgctac cgcccgcgtt gttcatggtc
    12901 tggctcttcg gtcaccacaa tcagacgggt acgacgaaga cgctgctggg tgaccgagtt
    12961 gtgatgtcgt cgtcggcgcg ccgcgtgaag atagaagtcg tccgcattac gtatggttga
    13021 cagccggatc ttggagatgg cgcggtccag gtcttgcggg tgagggcctg atcgttgtgc
    13081 aagtcccgta gaaccggacg caagggagcg tcacgatggg catgtcgaaa gccaaggttc
    13141 ggatgcgaag caggcttcat gaggaagacg cggctgcgta ggtggagatg gggagacgag
    13201 aagagcaaag acgcgggtgt ggtgattggc gaacccgcgt cactcaagtt gcatcacggc
    13261 gcattgcatt cggtcgggca tttggtcgcg aggcagtcat cgaggacacc aatcgccgcc
    13321 gctgccgcgg gatattgggt accgcaggaa tccatgcagg ccggggtcgt acaattcccg
    13381 gcgcaatcca gataggcggt gattccgtca gcgtcagaag cgtcgatgca ttcctgttcg
    13441 cacttggcct tcatgcagtc gttgcaggcg gccatgctcg ggtcggtgaa gccgatggtg
    13501 cattggccgg tggttttgca ggctgcgtcg caatgctgct cccagcaagt gctgacggcc
    13561 gtcagcaaca ggtcttggtt acagttcgtt ccccccgtgc cggtgatgca agcaaggcag
    13621 ttttggttgg cttcgcaggc tttgatctgg ctgcagcagt tatcgcccaa gcaggtggcg
    13681 cacgattgat tgctcatctg gagtccgctg tcacaaattt gataagcagg accaaaaccg
    13741 gggcattgaa ctgcgcactt gtcgccaagg caatcgaagt agttctcgag agccgcgccc
    13801 tccgcagggt aggtctgatc acaagttttc atgcaagtgt tgtcattgca gccaatgacg
    13861 cagttgaggt gatcctcagc gtttggcaat tcagagtacg tctcgcaaac gtcgttgcag
    13921 tttgccgtgg c
//
LOCUS       DAOSOR010000078        13898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4432_length_13898_cov_4.458065, whole genome shotgun sequence.
ACCESSION   DAOSOR010000078 DAOSOR010000000
VERSION     DAOSOR010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 13898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13898
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4432_length_13898_cov_4.458065"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PK710_06225"
     CDS             complement(<1..190)
                     /locus_tag="PK710_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09346.1"
                     /translation="MREVKKKGTPRQNPTRLIDVLRSLPKAELESLAQRIGASIDRNL
                     RADGPMQMARKLVTMVELR"
     gene            456..1754
                     /locus_tag="PK710_06230"
     CDS             456..1754
                     /locus_tag="PK710_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT09347.1"
                     /translation="MILRDPVHGLIAFESDEERIIENLLETREVQRLRRIRQLGFTSL
                     AYPGAEHTRFAHAIGTAHVMKRLIQRLRDLQGSLPPEHRICRARAKDALAAALLHDLG
                     HGPLSHLFEDALPHSDPHEIWTERIILDPGSEVHAVLAKHDPNMPSRVAELIAGRHEL
                     PYLARAVSGTFDVDRCDYLLRDAHATGVRYGNFDLDWLHRSLRFAPFDAHAAPPALAI
                     DGVKGVAAIESFVLARLFMFQQVYFHKATRAAEWMVRAALSLAVQLLAKGVDLPSTPP
                     AFRSAAEGRCLSIDEYLELDDGVLTATLHAWESCDDPSLADLCQRLRSRRLFKTIEFV
                     GEESKNPALQQHLLLQAKRATERAGLDPAVYCGLDVASDVPFNDASDNLVVVFSKGTS
                     RRPAEVSFLLSRLDGEEMRRERLIVAPEVREQVLRMANLD"
     gene            1754..2353
                     /locus_tag="PK710_06235"
     CDS             1754..2353
                     /locus_tag="PK710_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09348.1"
                     /translation="MNRLHALCCFSVCFSASTGCFADGAWGRLKGDLAIQGDLGMVYD
                     GSHPGVMGAVAIRYLQSAGAYATWMMPVSKDADFERIGSVGVELRPLFLPRLLKDWQG
                     TSPRLNLWIDSFSIRMGTAFAHQGPYAQGPGFETGVGMGIPILPQAQGPWIGLSGLWR
                     WSQDAMHTRQNSDFVCTMTLGWQTILYSGLVDWKDTRTP"
     gene            2350..3567
                     /locus_tag="PK710_06240"
     CDS             2350..3567
                     /locus_tag="PK710_06240"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HOT09349.1"
                     /translation="MNSIRSSLEIAWHEALHDLDPAHLVAQHLKTLDLGSVLVIAVGK
                     AAPAMAQGALQILDPKTNYLVITTDGTDTSRLPNQEQILFAAHPIPDPRSMHAATSAL
                     QLAQQSQKDSLLLLVSGGASALLCAPNHISLEMKRRVTGGLMRSGANIQELNTVRRHL
                     STVKGGGLARAFPRRVHNVILSDVIGGLPHDIGSGPGVADPTTLRDAHAIAEKYLEPS
                     LQEPCKQAFRETLEPSDDVLDRVTAEIVAAPKDLANGFVAKLRQQGWDAHGHPSQSLS
                     AEQLAGWLVNTAYTLSPGQGSVMACEPTLSVPQNAGRGGRAGWVALRTLQSLPDDVIV
                     WAAASDGVDGNSDHAGACVGGADKTRIDTTWVDLCLERRDDATAHQRLGTALEGKPTG
                     ANYTDIYGVFRLR"
     gene            complement(3559..4575)
                     /locus_tag="PK710_06245"
     CDS             complement(3559..4575)
                     /locus_tag="PK710_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09350.1"
                     /translation="MKTSEEPTSRGLSAIQEGRKRRAEFPRIPPRFFLWMFVILAGWA
                     IIYWKIDQNELESWRNRLLSRQRAVDAELGPRYNPVRDQLEVWIMEAAGPYAGDSISA
                     EAKSSPFRSKPGTYLRLYIDDAKQQESLRRAALESLRDGFTSCFTTFPNPDPFKGPAC
                     KKNQECPPGQHCNEVFHCSEPAQPYNLRVAYRAARVLSDSWVNDVREASNDMRLRLLE
                     RDFDSAVQEDIPLAIDLLVRAQYFLLVLDENTDLAATEVPDAGTYSESLQAVPHPVRV
                     FAWDLVSKKLLFRVRTEIVSKVGITTDSATTRIAVQRQSNNCAIAVEVRRLLGDESAG
                     APSP"
     gene            complement(4600..5100)
                     /locus_tag="PK710_06250"
     CDS             complement(4600..5100)
                     /locus_tag="PK710_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOT09351.1"
                     /translation="MQNKVSGGGARSVNTSLALIPLIDFLVVVVLFLLMSFSASGEIP
                     VDKNTKLPSAENTLDMIDAPVVAITGTNILVDGSPAGSTRPIEDSGRLQKIDDLHNIL
                     KNKRELWKQLNPKAESPGVVVMQVDHRVSALVVKSVFQTAAFAGYPNVSFMVGQLPKT
                     KKGGEH"
     gene            complement(5198..5761)
                     /locus_tag="PK710_06255"
     CDS             complement(5198..5761)
                     /locus_tag="PK710_06255"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOT09352.1"
                     /translation="MGGVSVDSGGKSGRRSVDSELNLIPMIDLFVVCVLFLIMTAVWS
                     QMARIEANAQVPGPPRTDEEVEKVEPEKMLTVEMKQDDRFVLIWKSGATVHATSEVPR
                     KGQPITLGTETVMRYPDLATEIAKQWKEYGSHQNDNDRKVDQAIISVDNRAPFSDIIA
                     VIDAIYTLKRTYKGAEINAFQVTFSAR"
     gene            complement(5807..6466)
                     /locus_tag="PK710_06260"
     CDS             complement(5807..6466)
                     /locus_tag="PK710_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOT09353.1"
                     /translation="MSGLWKSYQDGGWGMYPITFWSIVAIGIIVERAIVLYSQSSINK
                     NMFLASMQKCILAGDIAKAIKMCSAANAPMARIVQAGLVKVNRPDEEVQAAMDEAALR
                     ELPRITTRTGYLALLANLAMLSGLLGTITGLIKAFGAVGGESVDPSQKARILAEGISE
                     AMNCTAFGLMVALISLIGFALLNGKTQQLEDDINEATVQVLNLVIANRQKVNLQGVPQ
                     A"
     gene            7028..7723
                     /locus_tag="PK710_06265"
     CDS             7028..7723
                     /locus_tag="PK710_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOT09354.1"
                     /translation="MRLVLMLAALQEASAGGGEIGFMEAYKKSPWFLTMNMVVSVVVI
                     SIIAERFLYQLTKYRVNSKEFFSQVKKLVTGNNIDRAIKLCEASDYPILQLVKAGLTH
                     ANKTPDEIDAALSERFSELKPAIERRIGSLWSLANIATLIGLLGTVTGLIATFAAVSA
                     QGLSQAEKQRLLSLGIAEAMWNTAFGLGIAVACMIAHLILSQRAKKLAHELEATMEKV
                     FNLLAITNKPTGY"
     gene            7745..8323
                     /locus_tag="PK710_06270"
     CDS             7745..8323
                     /locus_tag="PK710_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOT09355.1"
                     /translation="MAKSSLSASQRSKIRRLATPKEQSPEDEGGELNIIPFLDIIVNI
                     LIFVLATVAITFTATIETTPPSSGGKGPRIISESKSLNLTVLITNDGISFKAAGGSVA
                     PGCEGIGPGITIPAIGADPDGQPVLDYDAVTRCAKRLKDQSPDFKDETQIRITASNNI
                     SYRKLIGTIDAVRGSEPNKTDLFPDVLFAVPR"
     gene            8339..8953
                     /locus_tag="PK710_06275"
     CDS             8339..8953
                     /locus_tag="PK710_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOT09356.1"
                     /translation="MTPPAAAQPSVGGSVAQYKAELRKAKRRNERDHEIDFLNITAML
                     DMMTIILVFLLKNMASSSASIPQSKDLTLPAAFLQTEPHEEGVAVIVTKSQILVGDNP
                     QPVVQHWSRESVAQSGLDAIYKKSGINDLHIVPLAESLLHVREIDNRIRNERKSGSGT
                     SEAIVIVDETTPYRLLIEVLYTLGASSFGKYHLMVLSTKKSADG"
     gene            9040..11010
                     /locus_tag="PK710_06280"
     CDS             9040..11010
                     /locus_tag="PK710_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09357.1"
                     /translation="MIEIASILGAGALALGVAIILALPLLSRATRLRLITSSYAPAYA
                     TIRNYLRVFDRSVLALTALVVFAIGVGVGVVRSHQPEDPISSPLSLASSMALATALGA
                     LCSSLAVHINAIASVRGACRLADFSKHSASAGLLVGIRWAGAASVASMAIVMLCPSVL
                     MTITCLFVSDQQCVPLLIHLLPCFALGTVFPSIVSHFIGGVFVSACASLPNHRNTFST
                     IPTHLTVPATRVADTVSTTVMLSVAVTIVAHDSSGLAPSLMFAPFLVVTLGLVVTCIA
                     LWIVRSDTTESGTSAIARGLWGLIVLVAGVFAGASHWLFGSQGWRVAAACALGLTGSA
                     VTLFVGRWRAQHKHHERAAATWANDMLTAAAIGAVIATVAIVGDVLGRDQTTLPWTGI
                     TLGFAALAFIATAPYGLAIGNMESIVGGHRANHAEPQNMPTTLSSCADTAFGVTTHAA
                     GGFALAVWMTSVAIFRKTELIQEDWGYAVGLSAAAFVGMVFVVALVAVSLRALAATSC
                     VALAGSSPSDDSRRVDASLQEATRQGIPWMLMVTIVPALVWAALRWGARPALVAASTL
                     SAILGASMLGLVVSLGTSFSGIRRNMASGPKGPVDKPVDVDEKWTNNGPVDALNGGDL
                     LGTSLQTLVGPCIHASVKLLAAAALALAAHLF"
     gene            11068..>13898
                     /locus_tag="PK710_06285"
     CDS             11068..>13898
                     /locus_tag="PK710_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOT09358.1"
                     /translation="MLSSLSDALSTLSDRGLRRLLGARTFLRGLDYARRKVVEGVDVG
                     EMIATGRVRGSDAEPYVVNVELTPSGIQSNCTCPAFAKAGQHCKHVAALLITVRDQAR
                     GAMPRRPAPPAPQPVAALPSAEGKVRRRDKRRRPTAIHVLPPQFGAVPEPDAVASATG
                     IGAWLPPGKQTSPRPLEYRIHVRQGAVTITVLDTDARVPLLPSQALAWQAIQPSPDYA
                     PLRLLARLESGNPRHPAVDVRGEDVAELLPLLRERRVLLEPALMQLRFVDDPLVPRFD
                     LELAGNDALVVKVSFERASDKRRFALSQGGWFEGAPGWQIDTSAGTAHPLHRRVSPAV
                     LRRLLRSPTIAEPIANVADLIMTGLPRIASEVGAELPDLDGVAQVVDLEPSFRIHAQG
                     DLVGAEVFLYASYGEIENPLRASGLGPPVLIQPPSAETQGRIRCIRADIPAQQEAVQK
                     LLELGLTPHESGDHFVAKGDRATEFWSEGLGALPETWSLYVPDDLVDTQVRTAHITPF
                     GRISSGVDWLSVKLGFEAEGVGVSREEIARCLATGQKYVKMEDGSFAYINREQIQAVL
                     DREIELMTAAKGGKLPLAQAGRVKELEELLNSFGKCTSTPATKKMFERLAGIDSIAVV
                     RKPRTLKAKLRPYQELGISWLKFIHDIASGGVLADDMGLGKTLQTIALFLLVKTEKNA
                     ISCLVVAPTSVVGNWEREINRFAPTLKVMVWHGAERKQQEEELRTSEVVITSYALLRR
                     DADLLQSLDFTYCVLDEAQHIKNPLSATAQAAKRIKASRRLALTGTPIENRLSEIWSI
                     FDFVSPGLLGPLGKFEEKFSRPIDQGDSKAAARLRAIIHPFILRRTKLEVAQDLPEKI
                     EMDRICELAGDQRTIYLQVAQEVRAQVLGEVERQGLAKSQIHILAGLTRLRQAACDPR
                     LLGLPRQFTDEDSGKLMALRELVEECVAGG"
BASE COUNT         2939 a         4156 c         3709 g         3094 t
ORIGIN      
        1 ctcgtaattc aaccatcgtt acgagttttc gcgccatttg catgggaccg tccgcccgta
       61 gattccggtc gatggacgcc cctatgcgct gcgccaacga ctcgagttcc gccttgggta
      121 gcgaacgcag tacgtcgatg aggcgtgtcg ggttttgccg cggtgttcct ttctttttca
      181 cttcacgcat gattacggcc cctgagccga gaagtcccga atatctctat ccgtccaaga
      241 agttttcatc acgaaggcaa tggcccatga tgcgtcctac ccccgtcggc gcatgatgcc
      301 tctgcctccg gactctttcc tcccatttcg gatactatgc atcggtcgag gttacaacga
      361 tggactccct ttgcctacca ccaccttcaa cgcctttgga ctgctggggt tcgtccgtgg
      421 ttgcgcgctt tcgtgggttg tgagaacacg acaccatgat cctgcgtgat cccgtccacg
      481 gtttgatcgc tttcgagtcc gacgaggagc gaatcatcga aaatctcctc gaaacccgtg
      541 aagtgcagcg attgcgacga attcgccagc ttggtttcac ttccctggct tatccaggtg
      601 ccgagcatac tcgattcgcc cacgccatcg gtacggctca cgtcatgaaa agactgatcc
      661 agcgattgcg agacctgcaa ggttccttgc cgccagaaca tcgaatctgt cgtgcccgtg
      721 cgaaagatgc cctcgccgca gccttgttgc atgatctcgg tcacggcccc ttgtcccatc
      781 tattcgaaga tgcccttcct cactccgatc ctcatgaaat ctggacggaa cgcatcatcc
      841 tcgatcccgg ttccgaggtt cacgccgtat tggcgaaaca tgatccgaat atgccctcgc
      901 gtgtcgccga actcatcgct ggacgtcatg aactccctta ccttgccaga gccgtcagcg
      961 gcaccttcga tgtcgaccgt tgcgactatt tgctgcgaga tgctcatgcc accggagttc
     1021 gctacggtaa tttcgatctc gattggctcc atcgaagctt acgtttcgcc cccttcgatg
     1081 cacatgcagc tcctcccgcg ctggccatcg atggagtcaa aggagtcgcg gcgattgagt
     1141 ctttcgtcct ggctcgcctg ttcatgttcc aacaagtgta ctttcacaaa gccacgagag
     1201 ccgcggaatg gatggttcgt gcggcccttt ctctcgccgt gcagcttctg gcaaaagggg
     1261 tggacttgcc ttccactcca ccggcctttc ggtccgccgc cgaaggccgt tgcctttcga
     1321 tcgacgaata cttggaactc gacgatggtg tgctgacagc aacgttgcac gcttgggaaa
     1381 gctgtgatga cccgagcctt gcggacttat gccagcgtct tcgttcacga cgccttttca
     1441 agaccatcga gtttgtggga gaagagagca aaaaccccgc gttgcagcag catttattgc
     1501 ttcaggcaaa acgagccacc gagcgcgcgg gattggatcc cgccgtgtat tgcggcctcg
     1561 atgtggcttc cgatgtcccg ttcaatgacg ctagcgacaa tctcgttgtt gtcttttcca
     1621 aaggtacttc acgtcgccct gccgaggtct cctttttgct gagccgcctg gatggagagg
     1681 aaatgcgaag agaacgtttg atcgtggctc ccgaggtacg cgaacaagtt cttcgaatgg
     1741 cgaatctcga ctgatgaatc gactccacgc tctatgttgt ttttcggttt gcttttccgc
     1801 gagcacgggc tgcttcgccg atggcgcctg gggacggctc aaaggtgacc tcgccatcca
     1861 aggcgatctt ggaatggttt acgatggttc ccacccggga gtcatgggag ccgttgcgat
     1921 tcgttatctc caatccgccg gtgcgtacgc cacatggatg atgccagtga gcaaagatgc
     1981 cgacttcgaa cgaattggct ccgtcggcgt ggaactacgc cctctatttc tcccgcgcct
     2041 gttgaaagat tggcaaggca ccagcccccg actcaacctg tggatcgata gcttctccat
     2101 tcgaatggga accgccttcg cccatcaagg accctatgcc caaggtccag gtttcgaaac
     2161 cggtgtcggg atgggaattc ctattcttcc gcaagcgcaa ggcccgtgga tcggcttgtc
     2221 gggactgtgg cgctggtccc aagacgccat gcatacccgg caaaatagcg actttgtatg
     2281 caccatgacc ttgggttggc aaacgatcct ctattccggt ttggtcgatt ggaaagacac
     2341 tagaacccca tgaactcgat tcgatcctct ctcgaaatcg cgtggcatga ggcgttgcac
     2401 gatctcgatc ctgcgcatct cgttgcccaa cacctgaaga ccctcgatct cggctcggta
     2461 ctcgtcatcg ccgtgggaaa agcagcacca gccatggcgc aaggagccct gcaaatactc
     2521 gaccccaaaa cgaattacct cgtcatcacc accgatggca ccgatacctc ccgtctgccc
     2581 aaccaagaac aaatcctttt cgcagcgcac cccattcccg atcctcgttc gatgcacgcc
     2641 gccacaagcg cactccagct cgcacaacaa tcgcagaaag actccctgtt gctgctcgtc
     2701 tccggcggag cttccgccct gttgtgcgcg ccaaatcaca tctccttgga aatgaaacgt
     2761 cgcgtgacgg gtggactgat gcgaagcggc gcgaatatcc aagaactcaa taccgtgcga
     2821 agacatctga gcaccgtaaa aggcggcggt ctcgctcgag cgttcccaag acgagtccat
     2881 aatgtcatcc tcagtgatgt catcggggga ttgcctcatg atatcggttc cggtcctggt
     2941 gttgcggacc ccacgacctt gcgcgatgcc cacgcaatcg ccgaaaaata cctcgaaccc
     3001 tcgctgcaag agccatgtaa acaggctttt cgagaaactc tcgagccctc cgatgacgtg
     3061 ctcgatcgag tcaccgccga aatcgttgcg gcacccaagg acctcgccaa tggcttcgta
     3121 gccaaacttc gacaacaagg atgggatgcg cacgggcatc cttcgcaatc tctgagcgcg
     3181 gagcaattgg ccggatggct ggtgaacact gcatataccc tttcccccgg acaaggatcg
     3241 gtgatggctt gcgagccaac cttgtcggtt ccccaaaatg caggacgggg cggacgcgcc
     3301 ggatgggtcg ctcttcgaac gcttcaatca ctacccgatg atgtgatcgt atgggccgcc
     3361 gcttccgacg gcgtcgatgg caacagcgat cacgccggtg cctgcgtcgg cggagctgat
     3421 aagacgcgca tcgatacaac ctgggtcgac ctatgcctgg aacgtcgtga tgatgctacg
     3481 gctcatcaac gcctcggtac cgccctcgag ggaaagccca cgggtgcgaa ttacaccgat
     3541 atttatgggg tttttcgtct acggtgacgg tgcgcccgcg gactcgtcgc cgagaagcct
     3601 gcgaacttcg accgcaatcg cacagttgtt cgattgacgc tgaaccgcga tccgcgtggt
     3661 tgccgaatcg gtagtgatac caactttgga aacgatttcc gtgcggactc gaaatagaag
     3721 ttttttcgaa accagatccc aagcaaaaac tctcaccgga tgcggcaccg cttgcaaaga
     3781 ctccgaatac gtccccgcgt ccggaacctc cgtagcggcc aaatccgtgt tttcgtcgag
     3841 gacgagcaaa aagtattgag cccgaacgag caagtcgatg gcgagaggaa tgtcttcctg
     3901 aaccgcgcta tcgaagtccc gttcgagcaa acgtaagcgc atgtcattgc tcgcttcgcg
     3961 gacgtcgttg acccaggaat cggatagcac gcgcgcggcg cggtaagcga cacgaaggtt
     4021 atagggttga gcaggttccg agcagtggaa aacttcgttg cagtgctgtc ctggtggaca
     4081 ttcctggttt tttttgcacg cagggccttt gaaaggatca gggttgggaa aggtcgtaaa
     4141 acaagaggta aacccatcgc gtaacgattc gagagccgcg cgacgcaacg actcttgttg
     4201 cttggcgtcg tcgatataaa gtcgcaaata ggttccgggt ttcgaccgga aaggggagga
     4261 tttggcttcc gctgagatgg agtcaccggc atacggtccg gccgcttcca tgatccaaac
     4321 ttccaattga tcgcgcacgg ggttgtaacg aggacccaac tcggcgtcga cggcgcgttg
     4381 gcgagacaaa agacgattgc gccaagattc gagttcgttt tgatcgattt tccaatagat
     4441 gatcgcccat ccagcgagga tgacgaacat ccacagaaaa aatcggggag ggatgcgagg
     4501 gaattcagct ctgcgctttc ggccttcttg gatggccgag agccctcggg acgtaggctc
     4561 ctcagaggtt ttcatgcgcg agatgaacgg agagcggggt cagtgctcgc cgcctttttt
     4621 ggtcttcggc aattgtccga ccatgaaaga gacgttggga taaccagcga acgccgcggt
     4681 ttggaagacg gacttgacga cgagagcgga gacgcggtga tcgacttgca tgacgacaac
     4741 accaggactc tccgctttcg gattgagttg cttccacaat tcccttttgt ttttcaggat
     4801 gttgtgaaga tcgtcgatct tttgcagacg accggaatcc tcgatcgggc gtgtggaacc
     4861 agcgggtgaa ccatccacga gaatattggt gccggtgatg gcgacgacgg gagcatcgat
     4921 catgtcgagg gtgttttcgg cgctgggcag tttggtgttt ttatccaccg ggatttcgcc
     4981 gctagcggag aaagacatga ggaggaagag aaccacgacg acaagaaagt cgatcagtgg
     5041 aatcaatgcg aggctggtgt tgacagagcg tgctccaccc ccggagacct tgttttgcac
     5101 gaacctgagc gggaccttgt gcatgagccg gcgtcccggc ttctgaatca ttgagtgttc
     5161 gaatttgcgt tttcctctag ccatgggtcc ctccctttca acgtgccgag aaagtcactt
     5221 ggaatgcgtt gatttccgcg cctttgtagg tacgtttcag ggtgtagatc gcgtcgatga
     5281 cagcgatgat gtcagagaag ggggcccggt tgtcgacaga aatgatggcc tgatcgacct
     5341 tgcgatcgtt gtcgttctga tgactcccgt actctttcca ctgtttggca atttccgtgg
     5401 cgaggtccgg gtaccgcatg accgtttccg ttcccaatgt gatgggctga ccttttcgag
     5461 gcacctcgga cgtggcatgg acggtagcgc ccgatttcca gatgagaacg aaacgatcgt
     5521 cttgtttcat ctccaccgtg agcatttttt caggctcgac tttttcgact tcttcgtcgg
     5581 tcctgggcgg gccagggact tgggcgttgg cctcgatccg cgccatttgc gaccagacgg
     5641 cggtcatgat cagaaatagg acgcagacaa cgaacaagtc gatcatggga atcaagttga
     5701 gttccgaatc gaccgaacgc cttccgcttt tgccgccact gtcgacgctt acaccaccca
     5761 tggtctcctc ctgcttgcta gccgtacatc gaacgtcgga agtcgactac gcttgcggaa
     5821 ctccctggag attgactttc tgccggttgg cgatcaccaa gttgagaacc tggaccgtcg
     5881 cctcgttgat gtcgtcctcg agctgctgcg tcttgccgtt caacaaggca aagccaatga
     5941 gcgagataag agcaaccatc agaccgaacg ccgtgcagtt catggcctct gaaatgcctt
     6001 ccgcaaggat tctcgccttt tggctaggat cgaccgactc cccaccgacg gcaccgaacg
     6061 ccttgatgag acccgtgatg gttcccaaca gaccagacaa catggcgagg tttgcaagaa
     6121 gagcaaggta acccgtgcgg gtcgtgatgc ggggaagctc gcgaagggct gcttcgtcca
     6181 tcgccgcctg aacttcttcg tcaggtcgat ttaccttgac gagacccgcc tggacgatcc
     6241 tagccatcgg cgcattcgcg gccgagcaca ttttgatcgc tttggctatg tcacccgcca
     6301 agatgcactt ctgcatcgat gccaaaaaca tgttcttgtt gatcgaagac tgggaataca
     6361 gaacgatcgc acgctccacg atgatcccga tcgcaacgat cgaccagaac gtgatcggat
     6421 acattcccca gccgccgtct tgatagctct tccaaagacc actcatctga ccttcctcct
     6481 cgggtgcctc tttctgtgca ctctatgcat gtttgcctcg ccatgatgat cgagggcaaa
     6541 ccactcgaac tcatattgtc aaatccgacc gcttaaaagc atcacgccat cgtgcgcgcg
     6601 taactcacct cgttgttgtt ggagttccaa cttccgtgaa acattgaatc gcaagataga
     6661 acaacgaacg caaagtgagc cgtttcgaaa aactcgggag atattgcaag gaccgtgagc
     6721 aagtcgtcaa gagtaatgtc gtgttttgtt ggttcttcgc gtgagaataa aaagttacac
     6781 aaaaaacctg tccacatgcc cggcttccac ccgaccacca cggttcaccc ccccctatac
     6841 aaggtgtttg acgccccgtc caaaccttcg tgcattcccc cgcacctcac gcttgcgttg
     6901 tccaactcca cccgtctata gtctcccctc atcggtggag gcatctcatc ggtttgcccc
     6961 tcgcacatca cgcgccccgt ggcgagatga tgctttcgca cgatgaccta gaaaggagcc
     7021 ttgaggaatg cgtcttgtgc tgatgttggc agccttgcag gaagcctcgg caggtggcgg
     7081 agaaatcggt ttcatggagg cctataaaaa gtctccctgg ttcctcacca tgaatatggt
     7141 ggtgtccgtc gttgtcatct ctatcattgc ggagcgcttt ctgtatcaac tcaccaagta
     7201 tcgcgtgaac tcgaaagagt ttttctcgca agtcaaaaaa ttggtgaccg gtaacaatat
     7261 cgatcgcgcc atcaaattgt gtgaggccag cgattaccca atcctccaac tcgtcaaggc
     7321 agggctcacc cacgccaaca aaaccccaga cgaaatcgat gccgctttga gtgagcgttt
     7381 ctccgaactc aaacccgcca tcgaacgtcg catcggctct ctatggtctc tcgccaacat
     7441 cgccaccctc atcggcctgc tcggcaccgt gaccggtctg attgccacgt tcgccgccgt
     7501 ctccgcacaa ggcctcagcc aagccgaaaa acagcgcttg ctgtcgctcg gtatcgcgga
     7561 agccatgtgg aataccgcat tcggcctcgg tatcgccgtg gcctgcatga tcgctcacct
     7621 tatccttagt caacgcgcga aaaaactcgc gcacgaattg gaagcgacga tggagaaggt
     7681 tttcaacctc ttggccatta ctaacaagcc caccggttac taacggcttc acggagttct
     7741 gccgatggcc aagtcatcgc tctcagcctc gcagcgttcc aaaattcggc gcctcgctac
     7801 gcccaaagag caaagcccgg aagacgaagg cggagaactc aacatcattc ccttcctcga
     7861 catcatcgtc aatatcctca tcttcgtgct cgccaccgtc gccatcacgt tcaccgcgac
     7921 catcgaaacc acaccacctt ccagtggtgg aaaaggaccg cggatcatct ccgaatccaa
     7981 atccctcaac ctgacggtgc tcattacaaa tgacggcatc tccttcaaag ccgccggcgg
     8041 ttctgtcgct cctggctgcg aaggcatcgg tccaggaatc accatccccg ctatcggcgc
     8101 agaccccgac ggccagcccg tgctcgatta cgatgccgtg acccgctgcg caaaacgcct
     8161 gaaagatcag tctccggact tcaaagacga aacccagatc cggatcactg cgagcaacaa
     8221 catcagttat cgaaaactca ttgggaccat cgacgccgtc cgcggttcgg aacccaataa
     8281 aactgacctc tttccggacg tgcttttcgc cgtcccacgc tgattggagc tgccaaggat
     8341 gacacccccc gcagccgcac agccctccgt aggcggttcc gtcgcccagt acaaagctga
     8401 gcttcggaaa gccaaacgac gcaacgagcg cgaccacgag atcgacttcc tcaacatcac
     8461 cgcgatgttg gatatgatga caatcatact cgtgttcctg ctcaaaaata tggcaagttc
     8521 gagcgcctcc atccctcagt ccaaagacct caccttgcca gccgccttcc tgcaaaccga
     8581 gccccacgag gaaggtgtcg cggtcatcgt caccaaatca caaatccttg tcggtgataa
     8641 tcctcaacct gtcgtccaac actggtcccg cgaatccgtc gcccaatcgg gcctcgatgc
     8701 catctacaaa aaaagcggta tcaatgatct gcacatcgtg cctctcgctg aatcgcttct
     8761 gcacgttcga gaaatcgaca atcggatccg caacgaaaga aaatcaggat ccggcacctc
     8821 cgaggccatc gtcattgtcg acgaaaccac tccctatcgc ttgctcatcg aagtgcttta
     8881 tactctcggc gcgtcgagct tcggcaagta tcacctcatg gtattgtcca ccaaaaaatc
     8941 cgcagacgga tagccggttg atttttccgc cactcgcatg gtacggcccg cgctgaaccc
     9001 cgtgcctctg tttgctcgct tactcatctt cgtctcatca tgatcgaaat cgcttccatc
     9061 ctcggcgcag gggcgctcgc tctcggagtg gcgatcatac tcgctctacc attgctctct
     9121 cgagctactc ggctgcgtct cattacctca tcgtacgccc ccgcctatgc aacgatccgt
     9181 aactacctgc gcgtcttcga tcgctccgtg ctggctctca ccgcgctcgt cgtattcgcg
     9241 atcggcgtcg gtgtcggcgt cgtgcgctcc catcaacccg aagacccaat ctcatcaccc
     9301 ctcagcctcg ccagttcgat ggcgctcgcc acggccctcg gagccctgtg cagctccttg
     9361 gccgtccata tcaatgccat cgccagcgta cgcggcgcct gtcgcctggc tgacttctcc
     9421 aaacacagcg catcggctgg cttgctcgtc ggtatccgtt gggccggagc tgcttctgtg
     9481 gcgtcgatgg ccatcgtgat gctttgtccc tccgtgctga tgacgataac ttgtctgttc
     9541 gtctccgacc agcaatgcgt acccctcctc atccacctgc tgccatgctt cgctctggga
     9601 accgttttcc cttctatcgt ctcccatttc atcggtgggg tattcgtcag cgcttgtgca
     9661 tccctaccca atcaccgaaa tacattctct acgatcccca cccatctcac cgtgcccgct
     9721 acacgcgtgg ccgataccgt gtccaccacc gtgatgctca gtgttgccgt aaccattgtc
     9781 gctcatgact cctcgggctt ggctccgtcg ttgatgttcg ccccgtttct cgtggtgaca
     9841 ctcggattgg tggtgacgtg cattgcccta tggatcgtgc gttcggatac gaccgaatcg
     9901 ggcaccagcg caatcgcacg cggcttgtgg ggactcatcg tgcttgtcgc gggcgttttc
     9961 gcgggggcgt cgcattggct cttcggatcg caagggtggc gcgtggccgc cgcttgcgcc
    10021 ctgggattga ccggttctgc cgtgacgctt ttcgtcggac gatggcgagc acagcacaaa
    10081 catcatgagc gagcggctgc tacatgggcg aacgatatgc ttaccgctgc cgccatcggc
    10141 gcggtgattg ccaccgtggc catcgtcggt gatgtccttg gtcgagatca aaccacgttg
    10201 ccatggaccg gaatcaccct tgggtttgcg gctttggcgt tcatcgccac cgcgccctac
    10261 ggcttggcga ttggaaatat ggaaagcatc gtcggtgggc atcgtgcgaa tcacgcagag
    10321 ccgcagaaca tgccgacgac attgagctcc tgtgccgata cggcattcgg agtcacgaca
    10381 catgcagcgg gtggttttgc tctcgcggtg tggatgacca gcgtggcgat ttttcgaaaa
    10441 acagaattaa tacaagagga ttggggctac gcggtggggc tgagcgcggc ggcttttgtc
    10501 ggaatggtat tcgtcgtcgc cctcgtggca gtgtcgctgc gcgccttggc cgccacctct
    10561 tgcgtcgctc tcgcgggtag ttcaccgtcg gatgactccc gaagggtcga cgcctccctc
    10621 caagaagcta cgcgacaagg aattccatgg atgctcatgg tgacgatcgt cccggcgctt
    10681 gtctgggcgg cgttacgttg gggggctcga cccgctttgg tggctgcatc gacgttgtcg
    10741 gcgatcctcg gagcatcgat gctcggactc gtcgtttctc tcggaacatc gttttcgggg
    10801 atacgacgga atatggcttc aggaccgaag gggcccgttg ataagcctgt tgacgtcgac
    10861 gaaaaatgga ccaacaatgg tcctgttgat gctctgaacg ggggtgattt gttagggaca
    10921 tcccttcaaa ccctggtcgg gccttgtatc cacgcttccg tgaagttgct cgccgcggcg
    10981 gcgttggcgc ttgcagcgca tctattctga aaccaaacag cccgtttccg agcttgatgg
    11041 acgggctctc ccccgcacgg gaggcttatg ttgtcgtctc tttcggatgc tctatccact
    11101 ctaagcgacc gcggtttgcg tcgccttctc ggtgctcgaa cgttccttcg ggggctcgat
    11161 tatgcccgcc gcaaagtggt cgaaggcgtc gacgtcggcg agatgatcgc caccggacgc
    11221 gttcgtggtt cggacgctga accttacgtg gtcaacgtgg aacttacgcc ttcgggaatt
    11281 caatcgaatt gcacctgtcc tgcttttgca aaagcaggtc aacactgcaa acacgtggcg
    11341 gcattgctca ttaccgttcg tgatcaggcg cgcggcgcca tgccgcgaag gcccgctcct
    11401 cccgcacccc aacccgttgc cgcactacct tccgcggaag gaaaggtgcg ccgtcgtgac
    11461 aaacgacgac gtcctaccgc cattcatgtt ctgcctcccc aattcggtgc ggtacccgaa
    11521 ccggatgccg tggcgagcgc caccggtatc ggggcttggc tacctccagg aaaacaaaca
    11581 agccctcgac ccctcgaata ccggatccac gttcgacaag gagccgtgac gatcacggtg
    11641 ctggatacgg atgcgcgcgt ccctctgctg ccctctcaag ctctcgcttg gcaagccatc
    11701 caaccaagtc cggattacgc acctttgcgg ttgttggccc gactcgaaag cggcaatccc
    11761 cgtcaccccg ccgtcgacgt gcgcggcgaa gacgtcgccg aattgttgcc gcttcttcgt
    11821 gaacgacgcg tgctgctcga gccagctctc atgcaacttc gtttcgtcga tgatccgctg
    11881 gtgccccgtt tcgatctcga actggctgga aacgatgccc tcgtcgtgaa agtaagcttc
    11941 gaacgagcca gcgacaaacg aagattcgcc ctgtctcaag gcggttggtt cgaaggagcc
    12001 cccggttggc aaatcgatac gtcggctggg acggctcatc ccctgcatcg tcgagtctcc
    12061 cctgccgtct tacgacgtct gcttcgttct cctaccatcg cagaaccaat agccaatgtg
    12121 gcggatctga tcatgaccgg actgccgcgt atcgcttccg aagttggagc tgaattgcca
    12181 gacctcgatg gcgtagcgca ggttgtcgat ctggaaccgt cgtttcgaat tcatgcgcaa
    12241 ggcgatctgg tgggcgcgga agtgtttttg tatgcgagct atggggaaat cgaaaatccc
    12301 ttgcgcgctt cggggcttgg tccacccgta ttgattcagc cgcccagtgc ggaaacgcaa
    12361 ggtcgaatcc gctgcattcg tgccgatatt cccgcacaac aagaagcggt tcagaaactc
    12421 ctcgaactcg ggctgacacc tcatgagtct ggcgatcact tcgtggccaa aggcgatagg
    12481 gcaacggaat tctggtccga aggccttgga gctttgccag aaacgtggag tctgtacgta
    12541 ccagatgacc ttgtggatac ccaggtgcgc acggcgcata tcacaccctt cgggcgaata
    12601 tcgtccggag tcgattggtt gtctgtcaaa ctcggtttcg aagcggaagg ggtaggggta
    12661 tcacgtgagg aaatcgcgcg atgcttagcc acgggacaga agtatgtgaa aatggaagat
    12721 ggctcgttcg cctatatcaa tcgcgaacag attcaagcgg tgctggaccg ggaaatcgag
    12781 ctgatgaccg ctgcaaaagg cggcaagctc cctcttgcac aagccggacg agtcaaagaa
    12841 ctcgaagaac tactcaactc gtttggaaag tgtacttcga cccccgccac caaaaaaatg
    12901 ttcgagcgtc tagccggcat cgattcgatc gctgtcgtgc ggaaacctcg aacactcaaa
    12961 gcgaaattgc gaccgtacca ggaacttggc atctcctggc tcaagttcat tcacgacatc
    13021 gcatccggag gcgttctcgc cgatgacatg gggttgggaa aaaccctgca aaccatcgcc
    13081 ctgtttttgc tcgtcaaaac cgaaaaaaat gcgatttcct gcctcgtggt cgcccccacc
    13141 agcgtcgtgg gtaactggga acgcgaaatc aatcgcttcg cgccaactct caaagtgatg
    13201 gtgtggcacg gtgccgaacg caaacaacaa gaagaagaac tcagaacctc ggaagtagtg
    13261 atcacgagct atgcgctgct tcgacgcgat gccgatctac tgcagtccct cgatttcaca
    13321 tactgcgtac tcgatgaagc ccagcacatc aaaaacccac tgagtgcaac cgcgcaagcc
    13381 gcaaaacgaa tcaaggcgag tcgtcggttg gcgctcaccg gcacgcccat cgagaatcgc
    13441 ttgtccgaaa tctggtcgat cttcgatttc gtgagcccag gactgctcgg acccttgggc
    13501 aagttcgaag aaaaattcag ccggcccatc gatcaaggag actcaaaagc agccgcgcgc
    13561 cttcgtgcca tcatccatcc gttcattttg cgaagaacga aactcgaggt ggcgcaagat
    13621 ctccccgaga aaatcgaaat ggatcgaatc tgcgaactgg cgggagacca acgaaccatc
    13681 tacttgcaag tcgctcaaga agttcgcgcg caagtgctcg gcgaagtgga gcgacaagga
    13741 ttggccaaga gccagattca tatcctcgcc ggtttgactc gattgcgaca agcagcctgc
    13801 gatccgcgat tgctcggact gccacgacaa ttcaccgacg aggactccgg aaaactcatg
    13861 gcattgcgcg aactcgtgga agaatgcgtg gccggtgg
//
LOCUS       DAOSOR010000079        13893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4434_length_13893_cov_4.093366, whole genome shotgun sequence.
ACCESSION   DAOSOR010000079 DAOSOR010000000
VERSION     DAOSOR010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 13893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13893
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4434_length_13893_cov_4.093366"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="PK710_06290"
     CDS             <1..1105
                     /locus_tag="PK710_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09359.1"
                     /translation="LREARPYATAAQQRLLDLQDRALDSTNPQEQRAFLDAWVSDGSS
                     LELALSLRPGEPREASLTPQLFLVDSSATAALQEPVATFAGTTASSARWVVATPLWMG
                     LVSPTPWATRMPDQDKTKIFQVPTAASAISHYLRGAMERAYVLDAEQKQDLLRCLGPS
                     QKIMQMAQAAVAPASSPTLHTVARAHATAAVLASPLSFSDLFADRTCVNLLGETYLLA
                     SFFELGLEGTEEASARLTPSALILSYASQQGAIQESLREGRTQLQPSDQESWARTMAA
                     LRDETKRIIEKREEGGAEKLLTQSHESPMGRWTHQARQQVQALGIARRFVWQPPLLIP
                     LRHDDGSLQDVTLDEEASWIEACLQAQGRERGP"
     gene            1271..1912
                     /locus_tag="PK710_06295"
     CDS             1271..1912
                     /locus_tag="PK710_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT09360.1"
                     /translation="MTVPQVDPGHYQDRSYDTRERFLSYWHQADEVRQRQPSNMLEVG
                     IGNGFLHRYLRSRGIDVHTLDIDERLGPDTVGSVLDLPFEQGSFDLVCCFETLEHLPW
                     ESFEPALRELRRVGRRWVLLSLPDITPYMRIDVMYKTPLREKKVRRLMELRGSPPVHA
                     FDGEHYWEIGKKGWPLRKILGVIDQVGLEVEGDYRVFELPYHHYFSTRIASGK"
     gene            1932..3392
                     /locus_tag="PK710_06300"
     CDS             1932..3392
                     /locus_tag="PK710_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOT09361.1"
                     /translation="MTKGPVKRALLTGSFWVSVSEGMTSLTDLARQVVAARVLLPGDL
                     GLMGIVLLQLSVLDALTKTGFDQALIQREKDVGSLMNVAWTWQLMRGFVLAAILAAAA
                     PWLARGYDLPILAPLIWVTCLHVVLQGMQNVGLIYFSRNLDFKTLFFINAVRSLVQAG
                     VAIPVILIRRDVWALVIGLVAGTLASVIVSFVAHPLRPKFEWNLDKARQLLKFGKWIT
                     GLTIMVFLIVQGDALFVSWYLGPVALGLYMFAYDVAGLPTTKISYVISRVAFPAYSRL
                     QDRPDEMRTVFEDVMRATMLVSGPVSVILFAIIPDIVHYVVGDKWIPVIPLVRILVIA
                     GWIRSFAALAGPLFQGANRPDLDFKMNLPRFLIAVLLIWPACAYGGLEGVSYIVLAGI
                     ATCLPTWFYGVKRIIGVSPWRVFHTNLLGWITTVVLGLTFLASRHLLHGNLPRDVASS
                     MLALGAWIGIMTLIGWLTPMSLFQEIKRLRSALRAK"
     gene            complement(3398..4267)
                     /locus_tag="PK710_06305"
     CDS             complement(3398..4267)
                     /locus_tag="PK710_06305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOT09362.1"
                     /translation="MSSPLPSVSVVIPAYRQAKFIEGCLDSVAAQTYQGPIETIVVDD
                     GCPENSGDIAERHALHPRVVRQNNGGVSAARNRGIAEASGTYLAFLDADDRWHPRKLA
                     VQIERLRAQGVAAFCFTRFRMAHGDGQPGDDSLHPSSTLQPSLRVLARNNFVGCSTVV
                     VDRSCVQKVGGFPDSEALRKGGQDYALWLRIATVAPLVFVPEVLVDYWVHPTSRVGSD
                     AVKNFEGAVFALQSFRAWNEQQFEVWTGSTYRALVGRRGLSMMQALWRGRRLDRRMWA
                     KAMRSWARALIES"
     gene            complement(4675..4748)
                     /locus_tag="PK710_06310"
     tRNA            complement(4675..4748)
                     /locus_tag="PK710_06310"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4712..4714),aa:Pro,seq:cgg)
     gene            complement(4869..7331)
                     /locus_tag="PK710_06315"
     CDS             complement(4869..7331)
                     /locus_tag="PK710_06315"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOT09363.1"
                     /translation="MSNGSGRERDLVLAPNEFAFISDQTKGNINAYVGPYKTSLANTD
                     QPVRFNDRTKRFEHCSLEESIQLFPTAPEGWYVVLKNPACDETTPKTGTVNGLHDLQV
                     GHKVNIPGPVSFALWPGQMVRIVKGHHLRSNQYLVVRVYDEDSAEKNWGKAVIKPQTD
                     AEASAKPSSELDRPELTMGKLLVIKGTEVSFYIPPTGVEIVRDVNDQYVREAVTLERL
                     EYCILLDEDGNKRFITGPAVVFPEPTETFVERSSQRKFKAIELNELSGLYIKVIAPYE
                     QDGRAFKVGEELFITGKEQMIYFPRPEHALIRYGEQALHYAVAIPAGEGRYCLDRDTG
                     KIQLIRGPRMFLADPRREVMVRRVLDSKLVRAWFPGNEEALAYNQQLQELMKEQGSGD
                     FVFDRTLHKGKTSPPSATQARGETIVADGFPRKTQFTPPRTITLDTKYEGAVTISVWT
                     GYAVMVVSKSGSRKVVVGPSTHLLEYDETLEGMALSTATPKSDDKLLHTAYVRVLHNK
                     VSDVVHAQTKDICSVEVRLSYRVNFEGDPNLWFNVENYVKFLTDHMRSLLRHAIKQVG
                     VQEFYASAADVIRNAVLGRPDETGTRTGRQFPENGMRIYDVEILDVTMGDEAIEELLT
                     EAQHAVVSQTLELEARKRRLEHVEQSEMIERRIAQYQAETKQLQMDLSIEERNKKLAV
                     ELADVMANIEARSRQLQGTVAEQKVLGEIKEAELGRAKAASDLELAVANQRMEQRLRE
                     LRDEVEAVVKKANAISPELIAALQAFGDRAMVERVAQSMAPLAILGGKSVAEVMASLL
                     KGTALERVLAPSVDTPVEKPKR"
     gene            complement(7403..8218)
                     /locus_tag="PK710_06320"
     CDS             complement(7403..8218)
                     /locus_tag="PK710_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09364.1"
                     /translation="MFSSSLCRFLLRSIGSSVIVTVASIACNHAASVDSRFDDPAAQR
                     PNGSRNLTVVFVNGVPTPVDSSLLREKEPPVAMQGEASVTWDVPSDWVQVPVSSPMRL
                     SEYRVDSSKPGGEPAELSIFHLGPSALRVDETLDRWASSFEGQQTTRSRRQVGSMPVY
                     IVEVSGTYRESEMGLEPGVEPRSHSSWALIGAIVVAPVGPFYFKLLGPRDTVHSSREG
                     FVHMLDSMRLGDAQASGASSAQGEPKPVGAVDAGETRAMPERGNPPAPSTSLR"
     gene            complement(8184..9398)
                     /locus_tag="PK710_06325"
     CDS             complement(8184..9398)
                     /locus_tag="PK710_06325"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOT09365.1"
                     /translation="MRLLIADTLHPLGIEELRHLGAEVIYEPEIDSETLPDAIHNVGI
                     LVVRSKRVTAKTIDASNQLNLIVRAGHGVGNIDVEAASTRGIYVASCKGMNAVAVAEL
                     TMGLILSLDRRIPDAVESVRAGRWEKGGFSEASGLKGKRIGIMGLGTVGREVLSRAKA
                     FGMIPYAWSRGLTASRAREFDLTCVKSPKDLASKVDVLTVHLAASERTRGIVSRDVLE
                     ALPHGATFINAARSELVDYEALLEIAPAKNLRVGLDVYADFPHVPMTTFAPPAFAPEV
                     IWYGTPHIGSQTQQAKRAVAAETVRIVRGFLVEGSVPNAVNVRTVHSARYQIVVRHYD
                     KIGALANTLNVLKRHGINVEELKTNVFDHSSAACTRINVVARPTEHCLQEIGAFHDEV
                     LHVQLVALPILA"
     gene            complement(9599..10954)
                     /locus_tag="PK710_06330"
     CDS             complement(9599..10954)
                     /locus_tag="PK710_06330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOT09366.1"
                     /translation="MKNLSLCSEFEQLCARGRAKRRGRIVSLISFLFVFLGAGPLRAQ
                     SQSQSPFHLVEQLARVLVLVENHYVQPVDRARMVEGAVKGMVAELDPHSSYLSPKEYE
                     EFRNDTEGHFVGIGVEVDARDGLITVIRPMEGSPAERAGLRPGDQVIAIDDWPTQAKP
                     LDQVVRRIRGQQGTPVSLLLRRPGVAQPLRVRIVRAQVHVRSVIGKRLEGNVAYVGIK
                     QFQRGTHQEFLQVLGDLRLQRDEPLEGMILDLRANPGGLVHESAQVADELLDGGIIFT
                     TRARGRVLDEVTASLGGAASRLPLAVLVNELTASAAELVAGAIQDHGRGIIVGAKTFG
                     KGSVQSIVELPEGAALKLTTTLYYTPLGRTIQAEGITPGVAVQQPGSGDGFPIVREQD
                     IEGHLASPNAAPRVSSPSTATTSEIMIPRHVPSNPVGRGDRALSIAYQLLLSDLIARK
                     K"
     gene            11079..11300
                     /locus_tag="PK710_06335"
     CDS             11079..11300
                     /locus_tag="PK710_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04563 family protein"
                     /protein_id="HOT09367.1"
                     /translation="MGDKENLKYEASKTDKRKQSLYFPEAMLQEIKEEAARLDRSLSW
                     IVQRAWKIARPDLRKLPSVNEIPDDETDG"
     gene            11414..11806
                     /locus_tag="PK710_06340"
     CDS             11414..11806
                     /locus_tag="PK710_06340"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HOT09368.1"
                     /translation="MRTIKTWLDSFGWQFLTMDETTLRIFRNENDDVDFFLRNTSHWI
                     LLKIVPAIPPTLTKPSDITRRLLAVNRDMRLAKHTLTKEGDIALAVELPKDSLEPAVL
                     RDAIERMHRYISHYRSYLSIVPPPWFHS"
     gene            complement(11823..13055)
                     /locus_tag="PK710_06345"
     CDS             complement(11823..13055)
                     /locus_tag="PK710_06345"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT09369.1"
                     /translation="MKRYDMDRMCLGAIALWLLVLGSCKSKGASSDSPQEQSTEIRPS
                     STVVLSAAPEQSEAAGTHRPVNKKLEVGQKVDIPSGAFLSGSTPGDRGRDASLEPALV
                     KVELGAYSIDRLPYPNDPSMEPRTGVTRREAEQLCAERKQRLCTELEWERACKGPEEN
                     AYSTGQGWDGACEKQPQACASGFDVVGMGTIREWTSSDWDVGDGESKTSAVLRGGRKG
                     VADTEHRCARRELADAQAQGMDLGFRCCGGAANAARVSKPPTLPTFRRAKLDASQVAS
                     MLRTVPQLADLGEVKFFQEPDDVNRVVARGDAGRQGNTLTTSPLLWSPALGEELLVMA
                     GLGASGSSFIAAFHRLPDDRYRIASTFVLKNDPGPIVLGFNGWVKNRLTWSSCWGCIG
                     EEGLIVHRDQGRIVIEQR"
     gene            13121..13381
                     /locus_tag="PK710_06350"
     CDS             13121..13381
                     /locus_tag="PK710_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09370.1"
                     /translation="MPHLMASQMAQLLVDSDLDELQEIVARWIQDAPSDSFRLRYQQF
                     GTHLLQLKRQLMSLPEPPQREDLETALQMMLEFAAQQKEPRG"
     gene            complement(13378..>13893)
                     /locus_tag="PK710_06355"
     CDS             complement(13378..>13893)
                     /locus_tag="PK710_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09371.1"
                     /translation="GTPRLELCNDGVCPEGLTCVQGFHGQYCTRSCDLAAPNCASGQT
                     CGDVGAGSGYCFMNEPCSAPEDCAEGLRCVNDGKNYCSPLCGLQGECPVGFSCAPEVG
                     VCFKEKSAGSVYSPPESGGCAYAPVSHSAGWFGLGMLLLMGSASRFRRAKRDDGKRDM
                     GTRVSARRRTI"
BASE COUNT         2998 a         4292 c         3611 g         2992 t
ORIGIN      
        1 gttgcgcgaa gccagaccct acgcaacggc cgcacagcag cgcttgctcg acttgcagga
       61 ccgggcgctt gattcgacga atccacaaga gcagcgggct ttcctcgatg catgggtgtc
      121 cgatgggtcc tcgctcgaac tagcgttgag ccttcgccct ggcgaaccgc gggaagcttc
      181 gctgaccccc cagttatttc tggtcgattc atcggctacg gcggcgctgc aggaacccgt
      241 tgccaccttc gcgggaacga ctgcgtcatc ggctcgatgg gtggttgcca cgccgctatg
      301 gatgggattg gtatcaccga cgccctgggc cacgaggatg ccggatcaag ataaaaccaa
      361 gatatttcaa gtacctacag cggcatcggc gatctcacac taccttcgcg gcgcgatgga
      421 acgggcttac gtattggatg cagaacagaa acaggatctg ctgcggtgcc tgggaccttc
      481 gcaaaaaatc atgcaaatgg cgcaagcagc agtcgcgccc gcttcctccc ccaccttgca
      541 tacggtcgct cgggcccatg ccacggcagc ggtgctcgca tcgccccttt ccttttccga
      601 cctatttgcc gaccgcacct gcgtgaacct gcttggggag acgtacttgc tggctagctt
      661 tttcgagctt ggtctcgaag gaacggaaga agcatcggcg cggctcaccc ccagcgcgct
      721 catcctttct tatgccagcc aacagggggc tattcaagag agcctgcgcg aaggtcgaac
      781 gcagctacaa ccttcggatc aagagagctg ggcccggacg atggccgcgc ttcgggatga
      841 aaccaagcga atcatcgaaa agcgggagga agggggcgcg gaaaaactgc tgacccaaag
      901 ccatgagtca ccgatggggc gatggaccca tcaggcccgg caacaagtgc aagcgctggg
      961 aatcgcgcgt cgattcgtct ggcagccccc cctgctcatc ccgctgcgcc atgacgacgg
     1021 gtccttacag gacgtgacgc tagatgagga ggcgtcgtgg atcgaggcat gtttgcaagc
     1081 ccaggggcga gaaaggggcc cttagcggtg ccgacggtct ttccatacac gtgcatctag
     1141 tggatatcat tagttatttt tttgattttc catcctcgca gcaaggggaa agaacggttg
     1201 cgtgccagat gcgaacgatg gggggtgtct tgtgcgaggc tcgaaccacg attagcctgc
     1261 cgcctgcgta atgacggtgc cccaagtcga cccaggccat tatcaagacc gttcctatga
     1321 tacgagggag cggtttctat cctactggca tcaggctgat gaggtgcgac aacgtcaacc
     1381 gagcaatatg ctcgaggtgg ggataggcaa cggttttttg catcgatatt tgcggtcccg
     1441 gggaatcgac gtgcatacgc tcgacatcga tgaacgtctc ggtcccgata cggtagggtc
     1501 ggtgctggat cttcccttcg agcaagggtc ctttgacttg gtgtgttgct tcgaaacgtt
     1561 ggagcatctg ccgtgggaaa gcttcgaacc agcgttgcgc gagcttcgcc gcgtgggacg
     1621 tcgctgggtg ctgttgagcc ttcctgacat cacgccctat atgcgaatcg acgtgatgta
     1681 caaaacgccg ctacgggaga aaaaggtgcg tcgcttgatg gagctacggg gctcgccgcc
     1741 ggttcatgcg ttcgatgggg agcattattg ggagatcggc aagaagggct ggccgttgcg
     1801 caagattctg ggcgtcatcg atcaggtggg tctcgaggta gaaggcgatt atcgtgtctt
     1861 tgagcttccc taccaccatt atttttcgac gcgcatagcg tcgggcaagt gacgattcgg
     1921 gcaaaggtgg catgaccaag ggtcctgtga agcgcgcctt gttgactggt tccttctggg
     1981 tatcggtgag cgagggcatg acatcgctga cggacttggc tcgccaagtg gttgcggcgc
     2041 gggtattgct tcctggcgat ctgggattga tggggatcgt gctgcttcag ctatccgtat
     2101 tggatgcgct gacgaaaacg ggtttcgatc aagcgctcat ccagcgggag aaggatgtgg
     2161 gcagcttgat gaacgtggcc tggacctggc agctcatgcg cggtttcgtg ttggcagcca
     2221 ttttggcggc ggcggcgcct tggctcgccc ggggttatga tttgccgatt ttggcgcctc
     2281 ttatctgggt gacgtgtctt cacgtggtct tgcaggggat gcagaacgtt gggctcatct
     2341 atttcagtcg caatctcgac ttcaagacct tgtttttcat caacgccgtg cgctcgttgg
     2401 tgcaagccgg cgtggcaatc cccgttatct tgattcgccg tgacgtttgg gccctggtca
     2461 ttgggttggt tgcgggaacc ctggcatcgg taatcgtttc gttcgtggcg catccgttgc
     2521 gaccaaagtt cgaatggaat ctcgacaaag cgcgacaact tctgaagttc ggcaaatgga
     2581 ttaccggttt gacgatcatg gtatttctga tcgtgcaggg cgacgctctt ttcgtgagct
     2641 ggtatttggg tccagtcgct cttggactgt acatgttcgc ctatgacgtc gccggtctgc
     2701 cgacgaccaa gatatcctat gtgatcagcc gggttgcgtt tcccgcgtat tcgaggttgc
     2761 aggatcgtcc ggatgaaatg cgcacggtat tcgaggatgt gatgcgagcg acgatgctgg
     2821 tgtcggggcc ggtgtcggtc atcctatttg cgatcattcc ggatatcgtg cattacgtgg
     2881 tgggggataa gtggataccg gtgattccgc tggtgcggat tttggtgatt gcgggatgga
     2941 tccgttcgtt tgccgcgctt gcggggccgc tgtttcaggg agccaaccga cccgatctcg
     3001 atttcaaaat gaacttgccc cgctttttga tcgcagtact gctcatctgg ccggcctgcg
     3061 cttacggggg gctcgaaggg gtctcctata tcgtgctggc cggcatcgcc acttgcctgc
     3121 ctacctggtt ttatggcgtc aaaaggatca tcggcgtcag cccatggcga gtctttcaca
     3181 ccaatttgct cggctggatt acgaccgtcg tactcggact gacgttcttg gcaagtcgcc
     3241 atctgctgca cggcaatcta ccgcgcgatg tcgcatcatc gatgctcgcc ctcggtgcct
     3301 ggattggcat catgacgctg atcggatggc tgacaccgat gagtctattt caggagatca
     3361 agcgtctccg cagcgccctg cgcgcgaaat gaattcctca cgattcgatc aaggcgcgag
     3421 cccacgaacg catcgccttc gcccacatac gccggtccag cctgcgacca cgccataacg
     3481 cctgcatcat cgacagaccg cgtcggccta ccaacgcgcg ataggtactg cccgtccata
     3541 cttcgaattg ctgttcattc cacgcacgaa aggattgcaa ggcaaagacg gcaccctcga
     3601 agttcttcac cgcatcgctt cccacgcgac tcgtcggatg cacccaatag tccaccaaca
     3661 cctccggcac gaatacaagc ggcgcgaccg tcgcaatccg aagccataag gcgtaatcct
     3721 gaccaccttt gcgcaaggct tcggaatccg gaaaaccccc taccttctgc acgcacgagc
     3781 gatcgacgac caccgtggag caacccacga agttgtttcg cgccaacacg cggagcgatg
     3841 gttgcaacgt cgacgaagga tgcagcgaat cgtcccccgg ctgtccatcg ccgtgggcca
     3901 tccgaaatcg ggtaaaacaa aaggccgcaa cgccctgggc tcgcagacgt tcgatctgga
     3961 cagccagttt gcgaggatgc cacctatcgt cggcatcgag aaaggctagg taggttcccg
     4021 acgcctcggc aatccctcgg ttgcgcgcgg cggaaacacc gccgttattt tggcgtacga
     4081 ccctgggatg caacgcgtgc cgctcagcga tatccccgga gttttcaggg catccatcat
     4141 cgaccacgat cgtctcgatg ggtccctgat aagtttgcgc ggccacgcta tccagacatc
     4201 cctcgatgaa cttcgcctgg cgataggcag gaatgacgac gctcacgctg ggaaggggag
     4261 aggacatcgg tggtcggtag gaataccgaa cggatggggc aagtcaagcc atgcgccctg
     4321 cgcgggatgc tgcctcggct gacgaagtca acggctactg cacggcggag gcgttgggat
     4381 cgttaggtta taactatgta tttttattat tattttctct tcttaggagg ctggtggatg
     4441 ggcagggcgg caggcgcttc gactcatgcg gaggctttga gaggatagga attggttgat
     4501 ggcttggagt gctggttttg ggagattgag gaaaggaaac ggaaggtggg tgtatggggt
     4561 ggcgaagggg ggcacgccgt ggaaagaggg agcaggggcg atgaacgagc gagacgaaag
     4621 ttggggccac aaaagacaag tagctgattt catgaaagaa accagctact cctgtcgggg
     4681 cgagaggatt tgaacctccg acaccacggt cccgaaccgt gtgcgctacc aggctgcgct
     4741 acgccccgtt aaggcgattg caaccaaatc aatggtccga atcagggggc gcagcatggt
     4801 gggtacagca tccattgtca agcgctcgtt gccttatccg ccacttttca cgttcgcgac
     4861 aatgggttct accttttcgg cttttccacc ggagtatcga ccgacggagc caacacccgt
     4921 tccaacgccg ttcctttcag caaagacgcc atgacctccg ccacactttt gcctcccaaa
     4981 atcgccaaag gcgccatcga ttgagccacc cgctcgacca tcgcccgatc accaaacgcc
     5041 tgcaacgctg caatcaattc cggactgatc gcattggcct ttttgaccac cgcctcgacc
     5101 tcatcccgca gttctcgcag ccgttgctcc atccgttggt ttgccaccgc aagttccaga
     5161 tcactggctg ctttcgctcg accgagttcc gcctccttga tctccccaag aaccttctgc
     5221 tcggccacgg tgccttgaag ttgacggctt ctcgcttcga tgtttgccat gacatcggcc
     5281 aactccaccg caagcttttt gtttcgctcc tcgatgctca aatccatctg aagctgcttc
     5341 gtttccgcct ggtattgcgc gatccgacgc tcgatcatct ctgattgctc cacgtgctcg
     5401 agacgtcgtt ttctcgcttc caattccaaa gtctggctta ccaccgcatg ctgcgcttcg
     5461 gtcaacaact cctcgatggc ctcatcgccc atcgtcacat cgaggatttc cacatcgtag
     5521 atcctcatgc cgttttcagg aaattgtctt cccgtacgtg tccccgtttc atcgggacgc
     5581 cccagcaccg cgttacgaat gacatccgcc gcagacgcat agaactcctg aacgcccacc
     5641 tgcttgatgg catgacgaag caacgaacgc atgtgatcgg tcaaaaactt cacgtagttt
     5701 tcgacgttga accaaagatt cggatctccc tcgaaattga ctcggtacga caatcgcacc
     5761 tccaccgagc agatgtcttt cgtctgcgca tgaaccacat ccgacacctt gttgtgcaac
     5821 acgcgaacgt acgcggtatg cagcaattta tcgtcgcttt tgggcgtggc cgtcgacaga
     5881 gccatgccct ccaacgtctc gtcgtactcc aacaggtgcg tgcttggacc cacgacaact
     5941 tttcggctcc cgctcttgct caccaccatc accgcatatc cagtccacac cgaaatggtg
     6001 acagccccct cgtatttcgt atccaacgtg atcgtgcgcg gcggggtaaa ctgcgttttt
     6061 cgaggaaatc cgtcagctac aatcgtctcc ccacgcgcct gggtcgcgga tggtgggctg
     6121 gtttttccct tgtgaagcgt tcgatcgaaa acgaaatcgc cagatccttg ctccttcatc
     6181 aactcctgca gttgttgatt gtaggctaaa gcttcttcat tacccggaaa ccatgcgcga
     6241 acgagtttgg aatcgagcac gcggcgcacc atgacttcgc gacgcgggtc cgcaagaaac
     6301 attctcgggc cccgaatgag ttggattttg ccggtatccc gatcgagaca gtacctgcct
     6361 tcgcccgcgg ggatggccac ggcgtaatgc agtgcctgct ctccgtaacg aatcaatgca
     6421 tgctcgggac gcggaaagta gatcatctgc tctttgcccg tgatgaacaa ttcctcgccc
     6481 actttgaaag cgcgtccgtc ttgctcgtag ggcgcaatga ctttgatata caagccgctc
     6541 agctcattga gttcgatcgc tttgaacttc cgctgcgatg agcgctcgac aaacgtttcc
     6601 gtgggctcgg gaaataccac cgccggaccc gtgataaacc gcttgttacc gtcctcgtcg
     6661 agcaagatgc agtactccag acgctcgaga gtgacggcct cgcgaacata ttgatcgttg
     6721 acatcgcgaa cgatttccac tcccgtcggg ggaatgtaaa aactgacctc cgtacccttg
     6781 atcaccagaa gctttcccat cgtaagctca ggacgatcga gttcggatga aggcttggca
     6841 cttgcttccg cgtcggtttg cggtttgatc accgcttttc cccagttttt ttccgcactg
     6901 tcctcgtcgt agacccgtac gaccaggtat tgattcgatc gcaagtgatg ccctttgacg
     6961 atgcgaacca tctgcccagg ccaaagcgcg aaagaaacag gccccggaat atttacctta
     7021 tggcccacct gcaaatcgtg cagaccgttg accgttcccg tcttcggcgt cgtctcatca
     7081 caggccggat tcttgagcac cacataccaa ccctccggcg cggtcggaaa tagctgtatc
     7141 gattcctcga gagaacaatg ctcgaaacgt ttcgtgcgat cgttgaacct cacaggctga
     7201 tccgtgttgg caagactcgt tttgtatggc cctacatacg cattgatgtt gcccttggtt
     7261 tgatccgaaa tgaacgcaaa ctcgttgggc gcaagaacga gatcccgctc acgaccgctc
     7321 ccgttgctca tatctatctc cttgcgaaag gaacccacct gtcgcgtcta tatcaccgaa
     7381 tcagtaaaaa tatccttgga attcagcgaa ggctcgtcga aggggcggga ggatttccac
     7441 gttccggcat ggcacgagtc tcgcccgcgt ccaccgcccc tacgggtttc ggctctccct
     7501 gcgcggaact tgccccagac gcctgcgcat cacccaatct catggaatcg agcatgtgga
     7561 caaacccttc cctcgaagaa tgcaccgtat cccgcggacc gagcagcttg aaataaaatg
     7621 gtccgaccgg cgccacgaca atcgcgccaa tcaacgccca cgatgaatga cttcgaggtt
     7681 ccacgcccgg ctcgagcccc atctccgact ctcgatacgt tcccgatacc tccacgatat
     7741 acaccggcat cgagccaacc tgtcgacggg aacgcgtggt ttgctgccct tcgaaagagg
     7801 atgcccagcg atcgagcgtc tcatccactc gcaacgccga aggcccaaga tgaaaaatag
     7861 atagctccgc aggctcacca cccggcttcg atgagtccac ccgatactcc gacaatcgca
     7921 tcggactcga taccggcact tgtacccaat ccgacggaac atcccacgta accgatgcct
     7981 cgccctgcat cgcgaccggt ggttcttttt cgcgcagcaa cgatgaatcc acgggcgttg
     8041 gcacaccgtt cacaaacacc acggtgaggt tgcgcgaacc attgggacgc tgcgccgctg
     8101 gatcatcgaa acgtgaatcg acggacgctg catggttgca agcgatcgac gctaccgtga
     8161 cgatgaccga cgaaccaatc gatctaagca agaatcggca aagcgacgag ctgaacatgc
     8221 aatacctcgt cgtggaacgc tccaatttct tgcaagcaat gctcggtcgg acgcgccacc
     8281 acgttgatcc gtgtgcaagc ggcagaggaa tgatcgaata cgttggtttt gagttcttcc
     8341 acgttgattc cgtgcctttt tagcacattg agcgtattgg ctagcgcgcc gattttgtcg
     8401 taatggcgaa cgacaatctg gtatctggcg ctgtgaacgg ttcgtacatt gaccgcgttg
     8461 gggacagagc cttccacgag aaatccgcgg acgatacgga cggtttcagc ggccacggcc
     8521 cgcttggctt gttgggtctg agagccgatg tgcggagtac cgtaccagat gacctcggga
     8581 gcaaaagctg gaggagcaaa cgttgtcatg ggcacatggg gaaagtctgc gtagacatcc
     8641 agtcccaccc gcaagttttt ggcaggagcg atttcgagca acgcctcgta atcgacaagc
     8701 tcgcttcgag ccgcgttgat gaacgtagcg ccatgaggaa gcgcctcgag cacgtccctg
     8761 gaaacaatgc cccgcgtgcg ttcgctggcc gcaagatgaa ccgtgagaac gtccactttc
     8821 gaagcaagat ctttgggcga tttgacgcac gtaaggtcga actctctcgc acgcgatgcc
     8881 gtcagccccc gcgaccacgc atacgggatc atgccgaacg ctttcgcacg gctcaacacc
     8941 tcgcgcccaa ccgtccccaa ccccatgatg ccgatgcgct tgcccttcaa ccccgacgct
     9001 tccgaaaagc cgcctttttc ccagcgccca gcacgaaccg attccaccgc atccgggatc
     9061 cgacgatcca acgacaagat cagccccatc gtgagttccg ccacggccac cgcattcatg
     9121 cccttgcacg aggcaacata aatgcccctc gtcgaagctg cttcgacatc gatgttgcca
     9181 accccatgac ccgctcgcac gatgagattg agttggttgg atgcatcaat ggtttttgcg
     9241 gtgacccttt tggatcgaac gacaaggatg ccaacgttgt gaatggcatc gggtaaggtt
     9301 tcactatcga tctcgggttc gtagatcacc tcggctccaa ggtgtctcaa ttcctcgatc
     9361 ccaagaggat gaagggtatc agcaatgaga agacgcatga tggcagaccg tcaaggtatc
     9421 acaggccatg aacgtttccc atgccgccga tcaagcgctc atggatttac ccttaccgta
     9481 tccttgtccg cgaaaagcta gctgtcattt ctcacaacca ccatcttgac ccttgccgat
     9541 gcgcgggctt ggcaaggtgg tttcgtggtc accgcccgga acatcgcacg ccggtggtct
     9601 atttttttcg cgcaatgaga tcactcaaca aaagctgata cgcaatcgac aaggctcgat
     9661 cccctcgccc caccggattg ctcggcacat gcctcggaat catgatctca ctggtcgtcg
     9721 cagtagaagg cgaggaaact cgcggcgccg cgttcggtga agcaagatgt ccctcgatat
     9781 cttgctcacg aacaatggga aaaccatctc ccgagcccgg ttgctgcacg gctacgcccg
     9841 gcgttatccc ctccgcctga atcgttctgc ccagcggcgt gtagtacaac gttgtcgtaa
     9901 gcttgagcgc cgccccctcc ggcaactcca caatgctttg cacggaccct tttccaaatg
     9961 tcttcgctcc cacgatgatc cctctgccgt gatcttgtat agccccggcc acaagctccg
    10021 ccgcactcgc cgtcaattcg ttgaccaaca ccgctagcgg aagacgactc gccgcaccac
    10081 ccaatgacgc cgtcacctcg tccaacactc gaccacgagc gcgcgtcgta aagatgatac
    10141 ccccatcgag caactcatcc gccacctgcg ccgattcgtg aacaagacct ccgggattgg
    10201 cgcgcaagtc caaaatcatg ccttcaagag gctcgtctcg ctgcaaacgc aagtccccaa
    10261 gcacctgcaa aaactcctga tgagtgcccc gttgaaactg cttgatgccc acatacgcca
    10321 cgttgccctc gagacgtttc ccgatcacac ttcgcacgtg gacctgcgcg cgcacaatgc
    10381 gcacacgaag cggttgcgcc accccaggac gacgaagcag aagcgatacg ggtgttcctt
    10441 gctgaccacg aatgcgtcgc acgacttggt cgagcggctt ggcttgggta ggccaatcat
    10501 cgatggcaat cacttgatca cccggacgca aaccggcacg ctccgcaggc gatccttcca
    10561 tgggacgaat cacagtgatg agcccatcgc gtgcatccac ttccacaccg atccctacga
    10621 aatgcccctc ggtgtcattt cgaaactctt catattcttt cggcgaaaga taggaagaat
    10681 gcggatcaag ttcagccacc atccccttga ccgcgccctc caccatcctc gctcgatcga
    10741 caggttggac atagtgattc tcaaccaaca ccaaaacgcg agccaattgc tcgaccagat
    10801 gaaagggact ctgcgactgg gattgcgccc gcagggggcc cgctcccaaa aatacgaaaa
    10861 gaaaagatat aagagaaaca attcttcccc gcctcttcgc ccttccccga gcgcacaatt
    10921 gctcaaattc gctacacaaa ctcaaatttt tcatgggggc agcctacgcc aatccgcgcc
    10981 cgatgcacgc gccctttgat cttctatcgt agcagaggta ctttgtgccc tgccgatgtg
    11041 gcgctggcta cgacatgtat gacggaggtc cccgttcgat gggcgataag gaaaatctca
    11101 aatacgaagc ttcgaagaca gacaaacgca agcagagcct ctacttccca gaggccatgc
    11161 ttcaagagat caaggaagag gcagcccgtc tcgaccgatc tctttcttgg atcgttcaac
    11221 gagcatggaa aattgcgcgc cccgatcttc gcaaacttcc aagcgtcaat gaaatccccg
    11281 acgacgaaac ggacggctga ttgtttgatc gctcgaacgc aaaaatgaac ctcgtgaggt
    11341 gatgccaaga catcatgcgc ggttcgacgt ttgcctcgat tggcccccga agaaattggc
    11401 tttccccatg gagatgagaa ccatcaaaac gtggttggac tccttcggat ggcaatttct
    11461 cacgatggat gaaacgaccc ttcgtatctt tcgcaacgaa aacgatgatg tcgatttctt
    11521 tcttcgcaac acctcccact ggatcctgtt gaagatcgtc cccgcaatcc cccccaccct
    11581 caccaaacca agcgatatca cccgtcgatt gcttgccgtc aatcgcgaca tgcggctcgc
    11641 caagcatacc ctcaccaaag agggtgatat cgcattggct gtcgaactac ccaaagattc
    11701 tctcgaaccc gccgtgcttc gagatgccat cgaacgaatg caccggtata tcagccatta
    11761 ccgatcctac ttgtccatcg ttccaccacc gtggttccac tcctaaccac acctattcga
    11821 attcatcgct gctcgatcac gattcgccct tgatcgcgat gaacgatgag accttcttcc
    11881 ccaatacaac cccaacaact actccaagtc agacgatttt tcacccagcc gttgaatcca
    11941 agcacgatcg gccctggatc gtttttgagc acgaacgtcg atgctatccg ataacggtca
    12001 tcgggcagac gatggaacgc cgcgatgaac gacgatccag acgctcccaa acccgccatg
    12061 accaacagct cttcgccgag tgccgggctc cacaacaagg ggctggtcgt cagtgtattg
    12121 ccttgccttc ccgcgtcgcc gcgagccacc actcggttga catcgtcggg ctcttgaaaa
    12181 aacttcacct caccaagatc cgcaagctgt ggaaccgtcc tgagcatgct cgctacctgg
    12241 gaagcatcga gcttggctct gcgaaacgtc ggcagcgtgg gaggcttcga gacacgcgcc
    12301 gcattggccg cacctccaca acaacgaaac cccagatcca tcccctgggc ttgcgcatcc
    12361 gcaagctccc ttcgcgcaca acgatgctcc gtatccgcta cacccttcct tcctccacgc
    12421 aacaccgccg aggtcttgct ctcaccatcg cccacatccc aatcactcga ggtccattcg
    12481 cgaatcgttc ccatgcccac cacatcgaat ccgctcgcac acgcctgcgg ctgtttttcg
    12541 cacgccccat cccatccttg acccgtggaa taggcattct cttccggccc cttacacgct
    12601 cgctcccact ccaattccgt acacaaccgc tgcttgcgct ccgcacataa ctgctcggcc
    12661 tcacgtcgtg tcacccccgt gcgaggttcc atcgacggat cattgggata tggtagccga
    12721 tcgatcgaat aggccccaag ttccaccttg accaacgcgg gctccaagga agcgtctctg
    12781 cctcgatccc caggcgtgct tccagacaaa aacgctccac tcggtatgtc gaccttttgc
    12841 cccacctcga gtttcttgtt caccggacga tgcgttcccg ctgcctcaga ttgctccggc
    12901 gccgccgaca aaaccaccgt cgaagacggc cgaatctccg tactttgctc ctgcggcgag
    12961 tcggaagatg cccccttcga cttgcaactc ccaagcacga gcaaccataa cgcgatcgct
    13021 cccaggcaca ttcggtccat atcgtatcgc ttcattcgca ctgccatccc tatgcctcga
    13081 cccatgcgtt tcgtcaaaga ccgagctaga ctttccccca atgcctcacc tcatggcttc
    13141 ccaaatggct caactgctcg tcgatagcga tctcgacgag ttgcaagaga tcgttgcgcg
    13201 ttggatccaa gacgccccct cggattcttt ccgtcttcgc tatcagcagt tcggaaccca
    13261 tctattgcaa ctcaaacgcc agctcatgag cttgcccgaa cctccccaac gagaagatct
    13321 cgaaaccgca ctgcagatga tgctggagtt cgccgcacaa caaaaagagc cgcgaggcta
    13381 aatcgttcgt cttcgagcag agactcgagt tcccatatcc ctctttccat catcgcgttt
    13441 cgcacgccga aatcgggatg ccgatcccat gagcagcagc atacccaacc caaaccaccc
    13501 ggcagaatga gatacgggcg cgtaggcaca tccaccagac tccggtgggc tatacaccga
    13561 ccccgcgctc ttctccttga aacatactcc tacctccggc gcgcaggaaa acccgaccgg
    13621 acactccccc tgcagaccac agagcggcga gcagtaattc ttgccatcgt tcacacaacg
    13681 aagcccctcc gcacaatcct cgggtgccga gcacggctcg ttcataaaac aatagccact
    13741 gcccgctccc acgtccccgc acgtttgtcc cgaagcacaa ttcggcgctg ccagatcaca
    13801 cgaccgcgtg caatactgac catgaaatcc ctgcacacac gtcaacccct cgggacatac
    13861 cccatcgttg cataactcca gcctcggcgt ccc
//
LOCUS       DAOSOR010000080        13815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4458_length_13815_cov_4.840480, whole genome shotgun sequence.
ACCESSION   DAOSOR010000080 DAOSOR010000000
VERSION     DAOSOR010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 13815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13815
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4458_length_13815_cov_4.840480"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1803)
                     /locus_tag="PK710_06360"
     CDS             complement(<1..1803)
                     /locus_tag="PK710_06360"
                     /inference="COORDINATES: protein motif:HMM:NF017437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT09372.1"
                     /translation="MRRSHEYVTVEHLLFALTFEKVASDVIESCGGDKEAIQTELEDF
                     FEEHVPILPDDSKTTPKETIGFRRVLERAIHHVQSSEKTSLDGSDVLVAIYSEEDSHA
                     RFILERQGISRMDMVDYISHGGVQAEEDGEEQEDDDGEGRQNQKKSTRRALEQFTINL
                     TALARDGKLDPLIGRESELHRIMQVLCRRRKNNPVLVGDAGVGKTAIVEGLAQRIVLG
                     DVPEILADAEIFSLDLGALLAGTKYRGQFEERMKAALNALEKKPKPILFIDEMHMIMG
                     AGAASGSSMDVSNLLKPVLQQGSLRCIGATTHEDFRQHLERDRALVRRLQKVDIDEPS
                     VQDTVKILEGLKDRYEQYHQVQYTQEALQSAAELSHHHITERFLPDKAIDVVDEAGAA
                     NQMRQKEERKPLLDKADVQQVVSRIARIPDLAATRSEKDRLRDLRERLGGVIFGQDHA
                     IDSVVNAIKLSRAGLGMPNQPVGSFLFVGPTGVGKTEVARQLATLLGIGFLRYDMSEY
                     MEKHAVSRLIGAPPGYVGFDQGGLLTSAIRKTPHTVLLLDELEKAHVDLFDILLQVMD
                     RATLTDNNGREADFRNVILIMTSNAGARDMSRKGI"
     gene            1939..3081
                     /locus_tag="PK710_06365"
     CDS             1939..3081
                     /locus_tag="PK710_06365"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOT09373.1"
                     /translation="MVTATVSVRVDRERARGPWLVSVDHRPLAAPPRLWQFFDAWGAS
                     ACEKPSVFADRASMTGVHATAPSHTTSPVVSSFAFETWFANLTGLQPSTIRNTVISII
                     VVAVVLLLRTVVLRLIQRHISDTTRHYHVRRAINYASSALLLLAVARVWFKGLQEIEI
                     ILGLASAGIAISLRDPFVSLSGWLFILLRRPFVIGDRIQVGDTFGDVVDIRMFQTYLL
                     ECGNWVDADQATGRIVMVPNAVVFTTPIANYTHGFAYIWDEVVVRLTFESDWKRAKKL
                     LTTIAEQHTQSSSADAEEQIRQAASEHLIFFKKLTPIVYTSVKEWGIQLTVRYLSPVR
                     QRRGLSQTVWEAILEAFANEPNLRFAYPTTRFFDRIAEEKSESSTK"
     gene            complement(3088..3402)
                     /locus_tag="PK710_06370"
     CDS             complement(3088..3402)
                     /locus_tag="PK710_06370"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HOT09374.1"
                     /translation="MSDQDREFGSDILEQDDISTRTPRPFHVILHNDDYTTMDFVQQI
                     LVEIFHHTPAAAAQLMLQVHTQGRAIAGTFTRDIAETKAAEAMTLAREQGHPLRCSVE
                     PG"
     gene            complement(3415..3699)
                     /locus_tag="PK710_06375"
     CDS             complement(3415..3699)
                     /locus_tag="PK710_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cereblon family protein"
                     /protein_id="HOT09375.1"
                     /translation="MVYRIEVAGAHRHTFANPHGLVFQIGCFSEATGCRGTGPASDDF
                     TWFSGYEWQVGVCARCGIHLGWRFISHRTSFFGLIVDRLRDTSLHPSQQP"
     gene            complement(3881..5698)
                     /locus_tag="PK710_06380"
     CDS             complement(3881..5698)
                     /locus_tag="PK710_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09376.1"
                     /translation="MKRCRGVMKLGTLVVASCLAGLVACGTESGGGYSSSPTADAGAD
                     AHGEGGTEAQAETGPIEHKLSSILVEPTNSLVELDLNAVSSQPYTALGRFLDGTTEDV
                     TDKVTWTLSDPEIGVFSGASLDLMPMVSAGAKTTRVRAELDGVKGEAQLTVVAYRKTG
                     PKTDFFFVLPFDDPQGSQDKPLEFSTDVRSLDVFFAMDTTGSMLGEIKNLQQALINQV
                     ITPMQQQIPDSQFGAGAFEDFPVGNYGATKGSECGKGGSSLPDQPLKLFSVITSDANQ
                     VQNAVNQFDTGQGPIGCGLDWPEAMIEALYQISTGEGLSGPGLTNVPANHVGVGGVGF
                     RQGSMPVIIPITDAMSHAPGENAYCPESGESVNYTGPVAAVAHDRQQTKDALDAICAR
                     VVGVASIETALSPQCSGQADEEDFARATGARIPPEAWDTPARPANCAPGLCCTDMNGA
                     GRQPDPDGLCPLVFRVNANGQGLGGSVVTGLQMLTRFAAFDVVTETVGETEGLSGEAL
                     PPGTTTADFLLSIVPDSYEKPPPPPNLPEPTMDSVGFQNVTPGTLVRFQVTAFNDFVK
                     GTEQAQFFRATIKVLAGGCTDLDAREVLILVPPNPIVIK"
     gene            complement(5836..7164)
                     /locus_tag="PK710_06385"
     CDS             complement(5836..7164)
                     /locus_tag="PK710_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOT09377.1"
                     /translation="MSRRGPTARSVAAHVLLRVEKDQAFASASLDSELARAVQLTPAD
                     RRLATELVYGVLRCETFLDDRIERYTRKGVEKLDVEVRVHLRVAAYQILILDRIPAFA
                     AVSAAVDLVRDARGHQVSRFANAVLRKIATEVQDHGRVSMNDAVKSSIHPGLLRRVTQ
                     ALGGREQAEAMLSTGPFPPALGIRIHAGQDVDAWSRLLEQSGSNVRVQRGKVSPRCLC
                     LFGAGRPDDLPGFDTAWSVQEEGSQVLGAAVGARQGERVLDSCAGRGNKTLLLAENVG
                     PSGWVDAADLYEPKLDALVTRARALNLPVRNTYAVDWTLGTGDVPDGYDRVLVDAPCS
                     GIGTMRRRPEVAHRNLDRTLPALQELQKGILRHAITRCRPGGTVVYAVCSILEDEAEA
                     IVQAVSSEGTMEPAPFDDSAIELIRGKTALRLLPHIHGTDGYFLASFRKR"
     gene            complement(7165..8832)
                     /gene="rseP"
                     /locus_tag="PK710_06390"
     CDS             complement(7165..8832)
                     /gene="rseP"
                     /locus_tag="PK710_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOT09378.1"
                     /translation="MDLLYFIILVSSLIFVHELGHFVVAKMFGVKVLTFSLGFGPKII
                     RIRGKETEYCIGILPLGGFVKMLEERKHEPVLPEDAKRTLESQPLWRRVLIVIAGPTM
                     NLVFPILLYFVAFLGDRHFLPPTVGMVLPDHPASGVLEPGDRIMEVNGERITTYTEFN
                     RIVRKSPGIPLHLKVFRELGYIDVTVTPQPVVEPRELDLFETVGRIGVKPGAPAAAIG
                     VFDPQSPAYRSGLRTFDLVIQVGERPIERFIELERAFDSNRGETIPVAYLRPKAVEGA
                     LGGLVDLAVFEHGVAALTPSPEPGELLARTGIESSDLYAAVVPRGSAEAKADLRPGDK
                     IIEIDGQPVLTWHSFRDKIEADPETPHLLTWLRDGRRTSGTIQVRREDWVDEMGVLRS
                     SFVIRAQNWLPMANEALVENPAPIRWAATNALEETHDVLRFISVSIVRLMQGKLSFSM
                     LSGPLTIYEVAGEEGAKGPGYFLWVMALVSVNLGLINLLPIPVLDGGHLLFFLIEAVI
                     RRPLPLRVREVASIIGVLVLFALMAIAFKNDVERRWDVIVSQLKELF"
     gene            8990..9880
                     /locus_tag="PK710_06395"
     CDS             8990..9880
                     /locus_tag="PK710_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT09379.1"
                     /translation="MFRIDSLTGNTQRLDGGAMYGNCPRALWETWSPPDDRHRILLAC
                     RPFLIRDGTRTVLLEAGIGTFFEPKLQDRFGVVEKEPLLLQSLASLGVQPVDIDVIVL
                     SHLHFDHVGGMLTSWQENQQPELVFPKATVVVGKKAWERALHPHARDRASFIPRLHEL
                     LQATGKLELVEGPHCSALPENIYEFCYSDGHTPGLTMTRVRCPRGPLTFVGDLIPGMP
                     WVHLPITMGYDRYPELLIEEKKSLLDRVIAENGWLLYTHDPHACASRVRLSEKGKYEA
                     YEAIATMRWTDSDNPIERTQ"
     gene            9883..10434
                     /locus_tag="PK710_06400"
     CDS             9883..10434
                     /locus_tag="PK710_06400"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOT09380.1"
                     /translation="MTILDPSSVRIHVLTYKEGLLSAVAHDLWIDVTRFTVDVNEEKN
                     GVEVKVDASSLSVRCAMKEGEQRMDMLSAKDKRDIEKHIVEDVLVIKKHRDIRFHSRS
                     MMAKGEGFSVSGDLEVMGKTRSISFDLMPRDREWLEATLRIHQPDFGIKPFSAMLGTL
                     KIKPELDVVVHVSRNVWSDEPSR"
     gene            complement(10481..11461)
                     /locus_tag="PK710_06405"
     CDS             complement(10481..11461)
                     /locus_tag="PK710_06405"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOT09381.1"
                     /translation="MNNPLRVSSAGASDVGRRRKHNEDVILVREDLSLWSVTDGAGGH
                     NAGEVAAALAARSISNYMGATVRKAWDQPIYDPFGIPNGGRRLAAAIRKANKDVLEVA
                     RHQNKYQNMVCTAVAAALCHRSMLMHVAHVGDSRCYRLRSCQLEQLTLDHSLLIEVLE
                     REPDLDNTIVARMPRNVVTRALGIEDNVRVTFRSFPMLPGDRYLLCSDGLSGMVDHPT
                     IEAALTEPSSPKEAVDRLIALANENGGKDNISVIVIDVDADVDLVGWQAPLATTKPET
                     LDSDIPEIQVLGTDESDTVGQRLSLIESFPPDLIEPIGELFKKNKDDQDD"
     gene            11657..12814
                     /locus_tag="PK710_06410"
     CDS             11657..12814
                     /locus_tag="PK710_06410"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT09382.1"
                     /translation="MASAPEPTPSQPSMVCPRCRATYDKHKTFCPVDGARLTDRLEHD
                     ILEESPSAPCASIIAGRYQVGSLIGQGSMARVYSAKDLATQKNVAVKILARRFAESES
                     ERRRFFHEARAAFSIDHPNVVKVMDVGRRIDGRPFIVIERLEGESLGECLRRRKHLPV
                     ETALPILRDAALGLAAVHDAGIVHRDVKPDNIFLVGLPDAPEAIRLVDFGLAKLHGGT
                     TRTGKTVGTAAYMPPEQVLSEGVDARADIYALGVVAFRVLTGHLPFESPKDIDMLAQQ
                     VLVHAPPLHWLLEPVDPRLEALVAKAIRKHPDNRYPTMRALAQDLQHILDGSSPAYSS
                     ALSVEPDAYLPVTDLGKATAFLFCQKLGVQPPWISGVNGQVSDPRNEDMRG"
     gene            12836..13255
                     /locus_tag="PK710_06415"
     CDS             12836..13255
                     /locus_tag="PK710_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09383.1"
                     /translation="MAYGHGSKLGLSILTLPEWLTRCCRGRMNRSPCDHGYRGKVSEM
                     SVELHVDGNDVRGELWLRLEPGTTVAARERLLDQHLGDILFEASVQMGVVLSSDPHCY
                     AQLTDQVNEEKQTRFWVRGRVEGDRLIPVRSHPRRRQ"
     gene            complement(13335..>13815)
                     /locus_tag="PK710_06420"
     CDS             complement(13335..>13815)
                     /locus_tag="PK710_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09384.1"
                     /translation="PAHAQQPQPYYYTEYSEQWYGWQNLAVDVPLLTTFVIAQTHGQD
                     TFALGTMGAFVVGSPIVHLAHHRVPPAVLSGFSHLLLPLGGYALLRPVVGEIAPSSSK
                     DTQIAAAVSITSLAALSLDVLWLAYDQTESEVRFESRARWIPHIALTTHSASLGWQF"
BASE COUNT         2911 a         3830 c         4044 g         3030 t
ORIGIN      
        1 aattcctttg cggctcatgt cccgggcacc cgcgttgctc gtcatgatga ggatcacatt
       61 gcggaagtcc gcttcccggc cgttgttatc cgtcaacgtg gcacggtcca tgacctgcaa
      121 gagaatatcg aagaggtcca cgtgggcctt ttcgagttcg tcgagcagca acacggtatg
      181 aggtgttttg cgtatggcgc tggtgagcaa tccgccttga tcgaatccga catagcccgg
      241 tggcgcgccg atcagtctcg aaaccgcgtg tttttccata tattcgctca tgtcgtagcg
      301 aagaaaacca atgccgagca gggtagcgag ctgacgcgcg acctccgttt ttcccacgcc
      361 tgtgggtccg acgaataaaa atgagccgac gggttgattg ggcattccga gtcccgcgcg
      421 ggacagtttg atcgcattga ccaccgagtc gatagcgtgg tcctgcccga agatgacgcc
      481 accgagtcgt tcacgcaggt ctcgcaaacg gtctttttcc gacctggtgg cggcaagatc
      541 gggaatgcgc gcgatgcgtg acaccacttg ctgtacatcc gccttgtcca gcagcggttt
      601 tcgttcctct ttttgacgca tttgattggc agcgcctgct tcatccacga catcgatggc
      661 tttatcaggg agaaatcgct ccgtgatatg gtgatgggaa agctccgccg cgctctgcag
      721 cgcctcctgg gtgtactgaa cctggtggta ttgctcgtaa cgatccttca aaccctcgag
      781 gatcttgacg gtgtcttgca ccgaaggctc gtcgatatcc accttctgaa gcctccgaac
      841 cagggcccga tcccgctcca gatgctgacg aaagtcctca tgggtcgtgg ctccgatgca
      901 acgcaaggaa ccttgttgca agacgggttt caacaggttc gaaacgtcca tggaactgcc
      961 gctggccgcg cccgcgccca tgatcatgtg catttcatcg atgaacaaaa tgggtttggg
     1021 ctttttttcc aaggcgttga gggcggcttt cattcgctct tcgaattgtc ctcgatactt
     1081 ggtgcccgcc agcaaagcgc ccagatccaa gctgaatatt tccgcatcgg ccaggatttc
     1141 cggcacatcg cccaggacga tccgctgcgc caatccttcg acgatcgctg ttttgccgac
     1201 tcccgcatcg cccacgagca ccgggttatt ttttcttcga cgacacagca cttgcatgat
     1261 ccgatgcaat tcggattcac gtccaatgag tggatcgagt tttccgtccc gggcgagggc
     1321 cgtaaggttg atggtgaact gttcgagagc ccggcgcgtg gattttttct gattttgcct
     1381 tccctccccg tcgtcgtctt cttgctcttc cccgtcttcc tcggcttgta cgccaccgtg
     1441 ggaaatatag tccaccatgt ccatacggct gattccctgc cgttcgagga tgaaacgcgc
     1501 gtgggaatcc tcttcggaat aaatggcaac gagcacgtcg cttccatcga gggatgtttt
     1561 ttcggaggat tgaacgtgat ggatggcgcg ttcgagtacg cggcgaaaac cgatggtttc
     1621 tttgggagtg gttttggagt cgtcgggaag gatgggtacg tgctcctcga agaagtcttc
     1681 gagttcggtt tggatggctt ctttgtctcc cccgcatgat tcgatcacgt ccgaggcgac
     1741 tttttcgaat gtcaacgcga agagcaggtg ctccacggtc acgtactcat gggagcggcg
     1801 catggcctcc cggatagaag actcgatggc gaaacgaaga ccgtggtttg acatgggtat
     1861 cgaccgagaa tgaaagactg ataaaagcca ggatggggag atgaccacca accaggcgat
     1921 tgtcaaggtg gggcggcgat ggtcacggct acggtatccg tgcgcgtgga ccgtgagcgt
     1981 gcgcgtggac cgtggcttgt gagcgtggat catcgacctc ttgcggcacc cccgcgcctg
     2041 tggcagtttt tcgatgcgtg gggagcttcc gcgtgcgaaa agccttcggt attcgcggac
     2101 cgcgcctcaa tgactggagt ccatgccacc gcgccatccc atacaacgag ccccgtcgtg
     2161 agttctttcg cgttcgaaac ctggttcgca aaccttaccg gtctacaacc atcgaccatt
     2221 cgaaatacgg tcatttctat catcgtcgtg gccgtcgtct tgctcctgcg gacggtcgtt
     2281 ctacgcctca tccaacgtca tatcagcgat accacccgcc actatcacgt acgccgcgcc
     2341 atcaactatg cttccagtgc tctgttgctg ctcgcggtgg cccgggtatg gttcaaagga
     2401 cttcaggaaa tcgaaatcat cttggggttg gcttcggcgg gcatcgccat ctccttgcgc
     2461 gatcctttcg tttccctctc cggctggttg ttcatcctgc ttcgaagacc cttcgtgata
     2521 ggagatcgaa tccaggtagg ggataccttc ggtgacgtcg tggacatccg catgttccag
     2581 acctacctgc tcgaatgcgg aaattgggtc gatgccgacc aggccaccgg tcgaatcgtg
     2641 atggtcccca atgccgtcgt ctttacgacc cccatcgcca attataccca tggtttcgca
     2701 tatatatggg atgaggtggt ggttcgcctc accttcgaaa gcgactggaa acgagccaaa
     2761 aaattgctga caaccatcgc agaacaacac acccaatcgt cgagtgccga tgcggaagag
     2821 caaatccgtc aggccgccag cgaacacctg attttcttca aaaaactcac tcccatcgtg
     2881 tatacgagcg tcaaagaatg gggaatccag ctcaccgtgc ggtacttatc tcccgtccgc
     2941 cagcgacgag gtctgtccca aacggtgtgg gaggccattt tggaggcgtt tgccaacgaa
     3001 ccgaacctgc gtttcgccta cccaaccacg cggttttttg atcgaatcgc cgaggaaaag
     3061 agcgagtcat ccacgaaatg aaacgaatca tccgggttcg acggagcatc gaagcggatg
     3121 tccttgttct cgcgccagcg tcatcgcctc cgcggccttc gtttcggcaa tatctctggt
     3181 gaaagtcccc gcgattgccc gaccctgcgt gtgaacctga agcatgagtt gcgcggccgc
     3241 cgctggcgta tgatggaaaa tctccaccaa aatctgctgc acaaaatcca tcgtcgtgta
     3301 atcgtcgttg tgcaggataa cgtgaaaagg tcgcggagtt cgtgtgctga tgtcgtcctg
     3361 ctcaagaata tcgcttccaa actctcgatc ttggtcgctc atgggcgtta ccccttacgg
     3421 ctgttgtgac ggatgtaaag aggtgtcgcg cagccggtcg acaatcaagc caaaaaatga
     3481 ggtccggtgg gagatgaatc gccaaccgag gtgaattccg caacgagcgc atacccccac
     3541 ctgccattcg tatccggaga accaggtgaa atcatcggaa gccggtcctg ttccccggca
     3601 tccggtggcc tcggaaaaac aaccgatttg gaaaaccaaa ccatgggggt tggcaaaggt
     3661 atggcgatgg gcacccgcga cttcgatccg atacaccatg gatgtaatcg gatgcctaca
     3721 ggttgcacaa accaacgtgg gttcggaagg gacttggtcc cgccgggtgt ggtgggatgg
     3781 ttccgagggg ggggtggtgg gtcgcgaggg ttcggaacgt agaaactgtt gcatattcaa
     3841 gtagttacaa ttcgggggcg aaggcggact tcgcgatgag ctacttgatc acgatggggt
     3901 tgggcggaac gagaatgagc acttcacggg catcgaggtc cgtgcatcct cccgcgagga
     3961 ctttgatggt ggcccggaag aattgcgctt gttcggttcc cttcacgaaa tcgttgaaag
     4021 cggtcacttg aaagcgcacc aacgttcccg gtgtcacgtt ctggaagccc accgaatcca
     4081 tggtgggttc tggcaagttg ggagggggcg gtggtttttc atacgagtcc ggcacgatag
     4141 agagcaggaa atcggcggtg gttgtgcccg gcggcagcgc ttcccccgac agtccttcgg
     4201 tttctcccac ggtttcggta accacatcga aagcggcgaa gcgtgtcagc atttgcaggc
     4261 cggtcaccac gcttccgccg agtccttgtc cgttggcgtt gaccctgaac accagggggc
     4321 acaggccatc cgggtcaggc tgtcgacctg ccccattcat atcggtgcag cagagccccg
     4381 gtgcacaatt ggccggacga gcaggagtat cccaagcctc aggagggatc cgcgcgccgg
     4441 tggcccgcgc gaaatcttcc tcatcggctt gcccggagca ttgcggcgac aatgccgttt
     4501 caatcgaagc caccccaacg acgcgcgcac aaatggcgtc gagcgcgtct ttggtctgct
     4561 gacgatcatg cgcgacggct gccacgggtc cggtataatt gacgctttct ccggactcgg
     4621 gacagtacgc gttctcgcca ggagcatggg acatggcgtc ggtaatcggg atgatgaccg
     4681 gcatggagcc ttgtcggaat ccgaccccac ctacgcctac atgattggcg ggaacattcg
     4741 tgagtccagg tccggaaagc ccttcgcccg tcgagatttg atacaacgct tcgatcatgg
     4801 cttcgggcca atcaagtcca cagccaatgg ggccctgccc ggtatcgaat tgattcacgg
     4861 cgttttgaac ttggttggcg tctgaggtga tgaccgaaaa cagtttcagt ggttgatcgg
     4921 gcagcgaact gccgcctttt ccacattcgg agcctttggt ggctccgtaa ttgcccaccg
     4981 gaaagtcttc gaaagctcca gcgccgaatt gtgaatcggg aatctgttgc tgcatcggcg
     5041 tgatcacctg gttgatcaag gcttgttgta agttcttgat ctcgcccaac atcgatccgg
     5101 tggtgtccat cgcaaaaaac acatccaggc tgcgaacatc cgtgctgaac tccaacggtt
     5161 tgtcttgtga tccctgcgga tcgtcgaacg gaagcacgaa aaagaaatcc gtttttggac
     5221 ccgtcttccg atacgcaacc accgtgagct gcgcctctcc tttgacccca tccaattcgg
     5281 cacgaacccg cgtggtcttc gctccagcgc tcaccatcgg catcaaatcg agactcgccc
     5341 cggaaaaaac gcctatttcc ggatccgaaa gcgtccacgt gaccttgtcc gtgacatctt
     5401 ccgtcgttcc atcgagaaat cgccccaggg ccgtatacgg ctgactcgac accgcattga
     5461 ggtccaattc gacaagcgaa ttggtcggtt cgacgagtat cgaggacaat ttatgctcga
     5521 tcgggccggt ttccgcttgt gcttccgttc ctccttcccc gtgggcatcc gctcccgcat
     5581 ccgcggtggg cgaactggag tatcccccgc ccgattccgt gccgcatgcc accagccccg
     5641 cgagacacga ggccacgacc agggttccga gcttcatgac acctcgacaa cgcttcatga
     5701 aaatctccgt atcgtcgata attccatgca gtatcgcaga cgatcgcacc atgcgccagc
     5761 gtatccatgg ggcaggagga cagaactcat gagacggctc ggcgcgcggt catgccctcg
     5821 acgggcgagc acacctcatc ttttccgaaa actcgcaaga aaatacccgt ccgtgccatg
     5881 gatatggggc agcaatcgca gagccgtctt gccccgaatc aattcgatcg cggaatcatc
     5941 gaaaggcgct ggctccatcg ttccctccga agataccgct tgaacaatcg cttccgcttc
     6001 gtcctcgagg atcgaacaca cggcgtatac cacggtgcct cctggacgac atcgcgtgat
     6061 ggcatggcga aggattccct tttgcagttc ttgaagcgct ggaagcgtgc gatccagatt
     6121 ccgatgagcc acctccgggc gtcgacgcat cgtcccgatc cccgaacagg gggcatcgac
     6181 caacacacgg tcatagccgt cgggcacatc accggttccc agggtccaat cgacggcgta
     6241 ggtgtttcgc acgggcaagt tcaaggcgcg ggcgcgagtc acgagggcgt cgagcttggg
     6301 ttcatacaaa tccgcagcgt cgacccagcc ggaaggacca acgttttcgg caagaagcaa
     6361 ggttttgttg cctcttccgg cgcaagaatc gagcacccgt tccccttgtc gagccccgac
     6421 cgcggcacca agcacttgag atccttcctc ttgcaccgac caggccgtat cgaatccggg
     6481 caagtcatcg ggacggcccg cgccgaacaa gcacaggcac cgcgggctta ctttgccgcg
     6541 ctgcactcgc acattggatc ccgattgttc caacaggcga ctccaagcat ccacatcttg
     6601 acccgcatga atgcggattc ccaaggccgg tggaaatggc cccgtcgaaa gcatggcttc
     6661 tgcttgttct cgccccccca acgcctgggt gacccgtcgc aacaatcctg gatgaatcga
     6721 ggattttacc gcatcattca tggatactcg gccatggtct tgaacttcgg tagcgatttt
     6781 tcgaagcacc gcattggcga agcgagaaac ctgatgaccc cgcgcatcgc gcaccagatc
     6841 cacagcggcc gaaacggctg caaatgctgg aattctatca agaattagga tttggtacgc
     6901 cgccacacgc agatggactc gaacctccac atccagcttc tccacccctt tgcgcgtgta
     6961 tctctcaatc cgatcgtcca aaaacgtctc acaccgcaac accccgtaca cgagttccgt
     7021 ggcgagacga cggtccgcgg gtgtcaattg caccgctcga gccagctccg aatccagcga
     7081 tgcggaggca aaggcctgat ccttttcgac ccggagcagt acgtgagcgg cgacagaacg
     7141 cgccgtcggt cctcgtcgac tcattcagaa aagctccttg agttggctca cgatgacatc
     7201 ccatcgtcgc tcgacgtcat ttttgaaggc gatggccatc aaggcgaaga gcaccagaac
     7261 gccgatgatg gaagcgacct cgcggacacg aaggggcaat gggcgacgga tgacggcttc
     7321 gatgaggaaa aagagcaggt gacctccatc gagcacgggg atgggtaata agttgatgag
     7381 gccgagattg acgctgacca gggccatgac ccaaaggaaa tatccggggc ctttggcgcc
     7441 ttcttcgccc gccacctcgt agatggtcaa gggacccgag agcatggaaa aactcagttt
     7501 tccctgcatg agacgcacga tgctcacgct gatgaagcgc aggacatcgt gggtttcctc
     7561 cagcgcgttg gtcgccgccc atcgaatggg agcggggttt tccacgagcg cttcgtttgc
     7621 catgggcagc caattttggg ctcggatcac gaagctggaa cgaagcaccc ccatttcgtc
     7681 aacccaatct tcacgacgca cctggatggt accgctggtg cgacgtccat cgcgaagcca
     7741 tgtaagcaga tgaggggttt ccggatctgc ctcgattttg tcgcggaaac tatgccaagt
     7801 aagcacaggt tgcccatcga tttcgatgat cttgtcgcca gggcgcaagt ccgctttggc
     7861 ttcggcggaa ccacggggaa ccaccgcggc gtacaagtcc gacgactcga tgccggtgcg
     7921 ggcgagcaat tccccaggtt cgggggaggg ggtcaaagcg gcgacaccat gctcgaacac
     7981 agccaagtcc accagacctc ccaatgcccc ttccaccgct ttgggtcgca agtaggccac
     8041 gggtatggtt tcaccccggt tgctgtcgaa cgcccgctcc aactcgatga accgctcgat
     8101 gggtcgttca ccgacttgaa tgaccaggtc aaaagttcgc aatccgcttc gatacgcggg
     8161 agattgcgga tcgaatacac cgatggcggc ggcaggagcg ccaggcttca ctccgatccg
     8221 tcccaccgtc tcgaacaaat ccaattcccg aggttcaacc acgggttggg gagtgaccgt
     8281 gacatcgata taccccagct ctcgaaacac cttcaaatga aggggaattc cggggctttt
     8341 gcgcacgatg cggttgaact ccgtatacgt ggtgatgcgc tccccattca cttccatgat
     8401 tcgatccccc ggctcgagca caccactggc cggatggtct ggaagcacca tgcccacggt
     8461 gggaggaagg aaatgccgat ctcctaaaaa agctacaaaa tacaataaga tagggaagac
     8521 gaggttcatt gtcggtccgg cgatgacaat caacacacgc cgccacaagg gttgggattc
     8581 gagtgttctt ttggcgtctt cggggagtac gggctcgtgt ttgcgttcct ccagcatttt
     8641 cacgaagccg cccaatggca ggatgccgat gcaatactcg gtttctttcc cgcggatacg
     8701 gatgattttc gggccgaatc ccaacgagaa ggtcaggacc ttgactccga acatcttcgc
     8761 caccacgaag tgacccaact catgcacgaa gatgagtgag ctgacgagga tgatgaagta
     8821 cagaaggtcc atgagcgaac aggggtatta ctgcgttgaa gccagcggag ccaggctgta
     8881 gaaacatggg gcggagcaga ctgggagctg gtcgaaggta ccgggtgcgc tgttgtcgtc
     8941 caccttccgg tcgtttggca agtcccgaac caccccgcgg agggttctca tgttcagaat
     9001 cgattcgttg accggcaaca cccaacgcct cgacggtggt gccatgtatg gcaattgccc
     9061 gagagctttg tgggaaacgt ggagcccgcc cgacgatcgc catcggattt tgcttgcctg
     9121 tcgtccattt ctcattcgcg acggcacgcg cacggtgctg ctcgaagccg ggatcggtac
     9181 atttttcgag cccaaactcc aagaccgctt cggcgtcgtg gaaaaagagc ctctcttgct
     9241 gcaatccctt gcgtccctcg gcgtacaacc cgtcgatatc gatgtcatcg tgttgtccca
     9301 tctgcacttc gatcacgtcg gtggcatgct cacctcgtgg caagaaaacc aacaacctga
     9361 actcgttttt cccaaggcca cggtcgtggt ggggaaaaaa gcatgggagc gtgcgttgca
     9421 tccccatgcc cgtgaccgtg cctcgttcat tccgaggttg catgaattgc tccaagccac
     9481 gggaaaactc gaactcgtcg aggggcccca ttgctccgcg cttcccgaaa atatctatga
     9541 attttgttat tccgacggtc atacgccggg gttgacgatg acccgtgtgc gttgtccccg
     9601 tggtccattg accttcgtgg gtgatttgat tcccggcatg ccttgggtcc acctgcccat
     9661 caccatgggc tatgatcgct atccggaatt gctcatcgaa gagaaaaaat cattgctcga
     9721 tcgggtcatc gcggaaaacg gctggttgct gtacacgcat gacccgcacg cttgcgcaag
     9781 tcgtgttcgc ctgtcggaaa aaggcaagta cgaagcttat gaagccattg cgaccatgcg
     9841 ttggacggat tccgacaacc ctatcgaaag aacccagtag ccttgacgat tctcgatcct
     9901 tcttctgttc gaattcacgt cctgacgtac aaggaagggc tactttccgc ggtggcccac
     9961 gatctgtgga tcgatgtcac tcgcttcacg gtcgatgtca acgaggaaaa aaatggtgtg
    10021 gaagtgaaag tcgatgcctc ttccctatcg gtgcgctgcg cgatgaaaga gggcgaacag
    10081 agaatggata tgctcagcgc gaaagacaaa agagatatcg agaagcatat cgtcgaagac
    10141 gtgctggtca tcaagaaaca ccgggacata aggtttcatt cccgatcgat gatggcgaaa
    10201 ggggagggat tttcggtgtc cggcgatctc gaggtgatgg gaaaaacgcg ttctatttcc
    10261 ttcgatctga tgccgcggga tcgggagtgg ctcgaggcaa ccctacggat tcatcaaccg
    10321 gatttcggca tcaaaccgtt ttccgccatg ctgggaacgt tgaaaatcaa gccggaactg
    10381 gatgtggttg tccacgtatc gcgaaacgtg tggagcgatg agccgtcccg ttgacgatgg
    10441 gctggtgaaa agagcaattt ttcgtttccc aagcgcgctt tcaatcgtct tggtcgtctt
    10501 tgtttttttt gaatagttcg ccgataggct cgatgagatc ggggggaaag gattcgatga
    10561 gagacaatcg ttgaccgacc gtatcggatt cgtcggttcc gagcacttga atttcaggga
    10621 tatccgagtc caaggtctct ggtttggtgg tcgccaaggg agcttgccat cccaccagat
    10681 cgacatcggc atccacatcg atcacgatga cgctgatatt gtctttgcca ccgttctcgt
    10741 tggcgagtgc gatgagtcgg tcaacggctt ctttgggcga ggagggttcc gtcaacgcgg
    10801 cttcgatggt ggggtggtcg accatgccac tcaaaccatc ggagcaaagc aggtatcggt
    10861 ctcccggtag catgggaaaa gagcggaagg tcacgcggac gttgtcttcg atgccgagcg
    10921 ctcgtgtgac gacattgcgt ggcatgcgcg ccacgatcgt gttgtcgaga tccggttctc
    10981 gttcgagcac ttcgatgagc agggagtggt ccaaggtgag ttgttcgagc tgacaggatc
    11041 ggaggcgata gcagcggcta tcgccgacat gggccacgtg catgagcatg gatcggtggc
    11101 agagcgccgc ggcgactgcg gtacacacca tgttttggta cttgttttgg tgacgagcga
    11161 cttcgagaac gtccttgttg gctttgcgaa tggcggcggc aagacgtcga ccgccgttgg
    11221 gaattccaaa cggatcgtag atgggttgat cccaagcttt tcgtacggtt gcgcccatgt
    11281 agttggagat cgaacgcgcg gcgagggcgg cggcgacttc tccagcgtta tgccccccag
    11341 cgccatcggt tacggaccaa agggacaagt cttcccgcac gaggatgacg tcttcgttat
    11401 gcttgcgacg acgtcccacg tcggaggcgc cagcgctgga tactcgaaga gggttattca
    11461 cgatggaccg tcgaaacagg atgatatgtc ggctgatggg gcgtcaatgc cgataggata
    11521 atgcaaagcg ttggggagga aaacttcacg gtggtcggtc gtgtcgacaa tggtcttggt
    11581 ttaccatccc aggctcgccg cggtagcatg ggtgtgctac ggtaaaagcg gtcgtcaacc
    11641 acccttttcg cgtgccatgg catcagcccc cgagccgacc ccttcccaac catcgatggt
    11701 atgtccgcgg tgtagggcaa cctacgacaa gcataaaacg ttctgtcctg tcgatggtgc
    11761 cagactcacc gatcgcctcg aacacgatat cctcgaggaa agcccatcgg cgccctgtgc
    11821 gagcatcatt gccggacgct atcaagtggg ctccttgatc ggtcaaggat cgatggcgcg
    11881 cgtatattca gcgaaagacc tggctaccca gaagaacgtg gccgtcaaaa tccttgcaag
    11941 gcgctttgcg gaaagcgaat cggagcgtcg acgattcttt cacgaggccc gcgccgcgtt
    12001 cagtatcgat caccccaacg tcgtcaaagt catggatgta gggcgtcgga tcgatggcag
    12061 accattcatc gtgattgaaa ggctggaagg ggagtccttg ggggaatgtc tgcgccgtcg
    12121 aaaacaccta cccgtggaga ccgcgctgcc gatattgcgt gatgcggccc ttgggcttgc
    12181 cgcggtgcat gatgcgggga tcgttcatcg cgatgtgaaa cccgacaata tttttttggt
    12241 gggattgccg gatgcgccgg aagcgattcg tctggtggat ttcggtcttg ccaagctcca
    12301 cgggggaacg acgcgtacgg gaaaaacggt gggaacggcg gcctatatgc caccggagca
    12361 agtactttcg gagggggtag atgcgcgggc ggatatttat gcccttggcg tggtggcgtt
    12421 tcgtgttctc acggggcatc tgcccttcga aagtcccaag gatatcgata tgcttgcgca
    12481 gcaggtgttg gttcatgcac ctccgttgca ttggttgctc gagccggtgg atccgcgtct
    12541 cgaagcgctt gtggccaagg ccattcgcaa gcatccggac aatcggtatc ccaccatgcg
    12601 ggcgttggca caggatcttc agcatattct cgatggctcg agccctgcgt attcttctgc
    12661 gctttcggtg gagcccgacg cttatcttcc cgtgacggat ttgggcaaag ccaccgcttt
    12721 tctattttgt cagaaactgg gcgttcaacc accatggata tcgggggtga atgggcaggt
    12781 gtccgatccg aggaacgaag acatgcgggg atgatgctgg tgttgtggcg gagtcatggc
    12841 ttatggacat ggctcgaagc ttgggttgtc catcctcacc ctgccggaat ggttgacacg
    12901 atgctgtcgc ggtcgaatga atcgaagccc ttgtgatcat ggctatcgag gaaaggtaag
    12961 tgagatgtcc gtcgaactgc acgtggatgg aaacgatgtc cgaggtgagt tatggcttcg
    13021 tttggaacca ggcacgacgg tagctgcgcg agagcgattg ctcgaccaac acctcggaga
    13081 cattttgttc gaagcgtcgg tgcagatggg tgtggtgttg tcttctgatc cccattgtta
    13141 tgcacagctc accgatcagg tgaacgagga gaaacagacg cgtttttggg tgcggggccg
    13201 tgtggaaggc gatcggctca tcccggtgcg ttctcatcct cgtcgacggc aataacgggg
    13261 gacggccacc ggatcgggga aatctaaaat aatatgtaaa ttcaattagt tgtgatggat
    13321 tggggcattc gttgtcagaa ctgccaaccg agggaggcgg agtgggtggt caaggcgata
    13381 tgaggaatcc agcgggcgcg gctttcgaat cgcacttcgg attcggtttg gtcgtaggcc
    13441 agccacaaca cgtcgagcga cagggccgcg agactggtta ttgaaacggc ggcagcgatt
    13501 tgtgtgtctt tggaggagct tggtgcgatt tcgccgacga cggggcgtaa gagtgcgtag
    13561 ccaccgaggg gcagaagcag gtgggagaag ccgctgagca cggctggggg tacacggtgg
    13621 tgtgcgagat gcacgatggg cgagcctacg acgaaggctc ccatggtgcc gagggcgaag
    13681 gtatcctgcc catgggtttg ggcgatgacg aaggttgtga gcagggggac atcgacggcc
    13741 aggttttgcc atccgtacca ttgttcggag tattcggtgt agtagtaggg ctggggttgt
    13801 tgggcgtggg cgggg
//
LOCUS       DAOSOR010000081        13734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4488_length_13734_cov_4.868338, whole genome shotgun sequence.
ACCESSION   DAOSOR010000081 DAOSOR010000000
VERSION     DAOSOR010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 13734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13734
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4488_length_13734_cov_4.868338"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="PK710_06425"
     CDS             <1..1073
                     /locus_tag="PK710_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09385.1"
                     /translation="HSRWCGGWGPATVPGYPIWSEVEPVLHGGERRTVKLSGIGLNRA
                     EVSGVLDRRQSHPDNSSLTDAPTRLGHGPRLIVLSDALSQLGLRSALFGGDGTVLAVG
                     PEGLYRWSQRSWSVLPTPPGLFMPDVRAMIRTPDGCSLLMGDHGMVVTLFPDGRPVWW
                     PLRQPDLRVFGAWMDRPDEAFLVGENTAGGGFFLHLSRQRESEIQRIDTPTVLRAIQR
                     TAGGKLIVCGDGGALFLWQSSILEPMAWERTGHLRAMAARPNDGLAIVGSGGHALHIS
                     PSMRVWIEPVQTTRDLTAVTMGFDGALWAVGEEGRVLRHDGTTWVRVSPSTPIPARLI
                     GVGATSSGVLVLGDDGLVVGVQ"
     gene            1195..2310
                     /locus_tag="PK710_06430"
     CDS             1195..2310
                     /locus_tag="PK710_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT09386.1"
                     /translation="MSIWTIAIHLPWAIALALALVRWLGLLSYGVAMGAVLLAYALLE
                     PLVFYPLPDKPRPAWRVDALERPYFVHVASVHVSAVGWLFNILNLQWKIDLWLGPFVA
                     YFMALWVFGWGSFVTSRRLRIRHLTLSVYGLPAAFEGYRIAHISDIHLGGFTPPSQVA
                     NWLERVRAVPVDLTVITGDVVTSGVQFHESIASLLGTLRSPDGVFFVPGNHDYFGNGM
                     PLFELLERHRVRVLRNEQVLLTRNDAPLLIAGMDDVWTGRGDLAVTLSQRHDRCTILL
                     AHDPIVFDDAARLGVSLMLSGHTHGGQLALPGLARHVNLTKRANRYSLGVYRNGRSTL
                     VVSGGLGCTGLPIRVGVPPEVGVITLVCARCTDAPSA"
     gene            complement(2307..4331)
                     /locus_tag="PK710_06435"
     CDS             complement(2307..4331)
                     /locus_tag="PK710_06435"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HOT09387.1"
                     /translation="MSAHGFRQASIAKLNEPGLGDKVHKATLHTTHGRDRLVESMPDW
                     DSLRHRAREARLRALDHHDALLETFMRALKARGIGLSLAKTPTEACEIITDLVNATGK
                     KVIKSKSMTSEEINLNHALEQAGAQVTETDLGELIVQLAEQAPSHVTAPAIHLSTEDI
                     ARIFRDKLGMQVPAWIDDPALVDDQTRNRLARDLSLTARKHLRERFLQADVGISGANF
                     LVAETGTVVLLENEGNILLTTALPKRHIVLAGVDKLIETEKDLGVLLRLLPVSATAQR
                     QSCYVSLLADAHPDMHVVLLDHGRSKLMADTQLRDVLTCIRCGACMNVCPVYRNVGGH
                     AYGAAYPGPIGALLMPHLEGMDRFGDLPFASSLCGACTQACPVQIPLHDHLLTMRARI
                     VQKGGPDSLRWPLRLASSAMHKASRMDRGTRLYPLVRKFAALHPTGKAWLESRTLPPP
                     AKESFRRWWQRQHVDRPATDTSESWKKSAMTEKASAPEPVDSPDDLLPLFQQRMLELG
                     PENTSEIQLFDAALVAEEYLRIRMEEHDPHTIAIQGETTEKREYTLGITRAEMLIADT
                     GGVLVDMRDRSQGHPIMLAETHIVVARPSQIVPTVAHALVARSVKRKSGAWGDYQVLV
                     TGPSRTADVEKVLVIPAHGPRKLVVVLCEEPVDLSSLRKPPEANRDAVDR"
     gene            complement(4328..5035)
                     /locus_tag="PK710_06440"
     CDS             complement(4328..5035)
                     /locus_tag="PK710_06440"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOT09388.1"
                     /translation="MRLGLFIPCFVDSFYPSVAIATVHILEHLGLRPEYPEQQTCCGQ
                     PHFNAGHREQARELARHFCEVFRPFDAIVCPSGSCTAMVRHHYPQLVDSPVSHRVFEL
                     CEFLVNQVGVTDLGACFEGRAALHIGCHALRELRIENAVHTLMRSVQGLCVVPTPSDS
                     FCCGFGGTFSVKFPEISTAMGQRKLGPIRQADVDFVISTDSSCSMHLGGLLDRQKIPR
                     PKLVHVAEVLASGLEAT"
     gene            complement(5068..5475)
                     /locus_tag="PK710_06445"
     CDS             complement(5068..5475)
                     /locus_tag="PK710_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09389.1"
                     /translation="MLKLGPRKKIDPNKIPKRSQPQPAHCGFCQKKIPRPKPDCRFSS
                     TLVGTCSHCGAWFIDDSTGKLGGEAWVVGLTLVAGPGGQAMQMREGIDFEQCMLAYDS
                     RRHEVDPHRDAKRYGVGRMWYFRALDANHAPAV"
     gene            5604..5678
                     /locus_tag="PK710_06450"
     tRNA            5604..5678
                     /locus_tag="PK710_06450"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5636..5638,aa:Cys,seq:gca)
     gene            5731..8136
                     /locus_tag="PK710_06455"
     CDS             5731..8136
                     /locus_tag="PK710_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09390.1"
                     /translation="MDVVAQKRAVSKALCLIPHHCEKMSPRMDAPLPLAGRYRYLRGL
                     RGASSGLIWLASDSVAGRTVVVSSVTPIRVAGLKNGLGLTHLHLATLLDLFDDISPGQ
                     IPGRVALRPGMAIAVAEHIAGQTLHERLESTPLSLHRAVGVVANIANALQALHAKGSV
                     HGALSPRSMVVLRNDGGVVPVLTNLRAAANGAYCSPERVRGGGPSTDDDVWALYSTLF
                     AAMTGDAPFEGKTREELAASILSGTVPNLVVHGVDDPDLQEIIRLGFHPDRNARQTSA
                     AVLENVLTAWLREHPSEHQERVSISAPFTSLPPPPIDDEDVAEGHRTLAVASIFSSGN
                     NASSSNADNDDDDDQATILLDAKANVAAAIATFGSPLGHPDDDATLQDQLPTNVMSTP
                     ELGAMLERIEAESARAPMPSVTRSMDPQVIDSFRAMSREQSDPQGADENSNLNAALPP
                     PAAIAPPLDPVAPPSDAIAMDDQVLAAAPAFPVSGPQKGWGPSEPTPPVIDPESLPLA
                     TEPLVPEQDWIDPAAVRASLSDPEPTSKKSSALRWVFGGLLLMVIAATLSFILAVVVS
                     GGTLPAPISSLPRVFRSLFHPADDTSHAATNPASNEIPPAPASSSSAPEQASASASAE
                     MMASASAPPTALSEEQLNACAQDFFPPNTFIRTVSFDFLCANDNPRKSAVMLHQQIIH
                     AGAGSVTDGMRLWSSLSWYELVVVTMIRNACCPGAAPLVLPEPGEPCASMVDVLQDAS
                     RGPCTQPHLGQRISRFEESVRCLYAHDRPRPYRYQGLLNSHQRATFEEFAGRIPPARC
                     APP"
     gene            8265..8993
                     /locus_tag="PK710_06460"
     CDS             8265..8993
                     /locus_tag="PK710_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09391.1"
                     /translation="MSWTWFIYSTRSMKETLALIDDANDVLDAWARARGPEGDEERIG
                     TCGTIEPGGPIPTTTQMRGILSPRGHAADPIVERLRSCRSSIALDRIRGTGLEHPLQV
                     SVVGYLLQRAGPSVVDWGDYQLVLGEQALAYVLQLPNHGPLDERPPPSVHPSSPLQNP
                     LQQRAIALLDALEQAHADVDRAIDFDRLARSFSDIQSRYIRFLLEEGAVDDASAARHL
                     GISESVLDQQADALLIALHNLIDP"
     gene            complement(9057..10010)
                     /locus_tag="PK710_06465"
     CDS             complement(9057..10010)
                     /locus_tag="PK710_06465"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HOT09392.1"
                     /translation="MTAPILLLAPMQGITDSIFREALTQLGGIDTCVSVYARVSHLPL
                     PAHSLLRICPEMATGGRTRHGTAVVLQLLGSDPSLMAETARRAVDAGAMGIDLNFGCP
                     VSRVNRHDGGAALLEEPRRITRVIRAVRDAVPSSIALSAKVRAGWSRTDEAPAIAQAV
                     EQGGAGWLTMHGRTRKQLYEGKADWEAIGKARRAVTIPVVANGDIRSVEDMTRCAEQT
                     GCERFMIGRGALAKPELFRWLKGLSEVPWQPRHRLAWLVSYGDACVAKGLGERTVAGR
                     AKAILRHMSEVNDCIYDIFIRQRQHDSWLELRQGILQDTSA"
     gene            complement(10232..11626)
                     /gene="xseA"
                     /locus_tag="PK710_06470"
     CDS             complement(10232..11626)
                     /gene="xseA"
                     /locus_tag="PK710_06470"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOT09393.1"
                     /translation="MATPPEIPSPSSEPTVWSVAELDRRLRFAAEKVSGGVWVQGEVQ
                     AVKTASSGHMYFTLKDEQQDAMIEAVAYRDDALRVRRVLVDGTRIMARGKATVWAPRG
                     KLQFVVEAARPVGKGALLEALERLKEKLRAEGLFDPARKRPIPTDAHRIGVVTSASGA
                     VIHDIIRVAFRRGSPNILLAPAQVQGEEAPRSLMAALGMLQRVQGLDVIIVGRGGGSL
                     EDLMAFNDERLVRFVAACPIPTISAVGHELDVTLLDWVADARAATPSQAAEMVVADRQ
                     QSARSLTHLRTRMQRAMTTHLREDRIAFEKLQTRLGDPKRSLDERAQGLDDLIQRAQG
                     ALRAKQISSRSRLEQWEKRLIARHPSTVLAKTRGDLDSLRLRIASAMQAKMQSQQAAL
                     SKTSARLEALSPLAVLARGYAIVARPDGQTLMDASHVAVADMVTVRLSKGRLSAEVVA
                     VTSEDSSEQVPLIT"
     gene            11771..13279
                     /gene="accC"
                     /locus_tag="PK710_06475"
     CDS             11771..13279
                     /gene="accC"
                     /locus_tag="PK710_06475"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOT09394.1"
                     /translation="MFNKVLVANRGEIACRVIRTLRDMGIRSVAVYSDVDRAALHVRM
                     ADEAVSVGEAPASSSYLRVDRILEAVQKTRAQAVHPGYGFLSENAAFAQACEDNSIVF
                     IGPPASAIAAMGSKTAARELMEKAGVPVVPGARCATVQEATEHAQQLGYPVMLKAAAG
                     GGGKGMRLVASEDEMANAWERAKSESKKAFGDDTVYLEKAIVKPRHVEIQVLGDRFGN
                     VVHLFERDCSIQRRHQKVVEETPCPVLTQDIVEKMGKVAVQGAKAVNYFSAGTFEFLL
                     SADQSFYFLEMNTRLQVEHPITEWVTGTDLVREMIRVAAGEPLSWNQEQIQRHGASIE
                     CRVYAEDPANDFLPSPGTIEQLRAPAGPGVRDDSGAYPGCTISSDYDPLISKLAVWAP
                     TREQAIARMRRALHEYVITGIRTNLAFHERLMNHPGFIQGVYDTGFIDAHRDQLMGFD
                     VPHEEMTEPLAVAVAMAAVGLERKMAATAHADCLDASRLSAWVATHRARRST"
     gene            complement(13300..>13734)
                     /locus_tag="PK710_06480"
     CDS             complement(13300..>13734)
                     /locus_tag="PK710_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09395.1"
                     /translation="PETGQDVTTDTVSDVSADQSVTPETSVPDAPVSDGSLFDLTMPD
                     VALNESGATLQGCYDCAVDHCTTEMQACEDDDKCRSILLCLFEDQCFGGPNGIDIGCG
                     WGCASKHGVTSPADPSVGIAMEAGKCISAACGDDCALPEGGI"
BASE COUNT         2714 a         4118 c         3959 g         2943 t
ORIGIN      
        1 ggcatagccg gtggtgtggg ggctggggac cggcaacggt ccccggctac ccgatttggt
       61 cggaagtcga acctgtcttg cacgggggag agcgtcgcac cgtgaaattg agcgggatcg
      121 gcttgaaccg tgccgaggtg tcgggagttt tggaccgacg gcagagtcat ccggacaaca
      181 gcagtctcac ggatgctcct actcggttgg gtcatgggcc gcggctgatc gtgctttccg
      241 acgccctttc acaattgggt cttcgaagcg cgctatttgg aggagatggg acggtattgg
      301 ctgtgggacc ggagggactg tatcgttggt cccaacggtc ttggtccgtg cttccaactc
      361 ccccgggcct ttttatgcca gacgttcgtg ccatgattcg cacgcccgat ggctgcagtt
      421 tgttgatggg cgaccacggg atggtcgtga cgctatttcc ggatgggcga ccggtatggt
      481 ggcccctacg acaaccggat ctgagggtat tcggcgcgtg gatggatcgt cccgacgaag
      541 cgtttttggt gggggaaaac accgcgggag gtggcttttt cttgcatctt tctcgacaac
      601 gggaatcgga aatccaacgg atcgatacgc ccaccgtgct gcgcgccatc caacgaaccg
      661 cagggggaaa gctcatcgtt tgcggagatg gaggagcgct tttcctttgg caatcctcca
      721 tcctcgaacc gatggcgtgg gagcgaacgg gacacttgcg agccatggcc gcccgtccca
      781 acgacgggct ggctatcgta ggaagcggtg gacacgcctt gcatatttcc ccttccatgc
      841 gtgtatggat cgaacctgtc caaaccaccc gggacctgac agccgttacc atgggtttcg
      901 atggcgcctt atgggccgtc ggggaggaag gacgcgtgtt gcgtcacgac gggacgacat
      961 gggttcgcgt gagtccatcg acgccgattc ccgctcggtt gatcggggtg ggtgccacgt
     1021 cttccggtgt tcttgtcctt ggtgacgacg gccttgtcgt gggagtgcaa tgatggttgg
     1081 tgacgcaagg cgaggaagcg gtgggatgct agccagccct tgccgttgtc ggattcccct
     1141 tatctgatgg cgaaggaaaa caagggtcca tggcatgcga aaatggtgcg gatcgtgtcg
     1201 atctggacga ttgccattca ccttccttgg gccatcgcgt tggccctggc cttggttcgc
     1261 tggcttggtc tgctgtccta tggcgtggcg atgggggccg tgctccttgc ttacgcgctt
     1321 ctcgaacctc ttgtattcta tccgcttccg gataaaccac ggccagcatg gcgtgtcgat
     1381 gccctggagc gcccgtattt cgttcatgtt gcatccgttc acgtcagtgc cgtgggatgg
     1441 ttgttcaata tattgaattt acaatggaaa atagatcttt ggctcggccc tttcgtggcg
     1501 tatttcatgg ccttgtgggt gttcggatgg ggttcgttcg tcacctctcg acggctcagg
     1561 attcggcatt tgacattatc cgtttacgga ttacccgcgg ccttcgaagg atatcggatc
     1621 gcccatattt ccgatatcca tctgggcgga tttacgcctc cttcccaggt ggctaactgg
     1681 ctcgaacgcg ttcgagccgt tcccgtcgac ctgaccgtca tcaccggcga tgtcgtgacg
     1741 agcggtgtcc aattccatga atccatcgcg agtcttctcg ggaccttgcg tagtcccgac
     1801 ggcgtatttt tcgtgccagg caaccatgac tacttcggca atgggatgcc gcttttcgaa
     1861 ctactcgaac gacaccgcgt tcgtgtgttg cgaaatgagc aggtattgct aacccgcaac
     1921 gatgccccgc tgctgatcgc aggcatggac gatgtgtgga cgggccgtgg tgatttggct
     1981 gtcaccctat cgcaacggca cgaccgatgt acgatacttc tcgcgcacga tcccatcgtt
     2041 ttcgatgatg ccgcccgctt gggcgtgagc ttgatgctca gcggtcacac ccatggcgga
     2101 cagcttgccc ttcccggact tgctcggcac gtcaacctta ccaaaagggc gaatcgctat
     2161 agcctgggtg tatatcgaaa cggacggtct accttggtgg tgagtggagg attgggctgt
     2221 accggacttc cgattcgcgt tggcgttcct ccggaagtcg gcgtgatcac cttggtgtgc
     2281 gcgcgttgca ccgatgctcc gagcgcttag cggtccaccg catctcgatt tgcctccggg
     2341 ggtttacgca acgacgaaag gtccaccggt tcttcgcaaa gcaccacgac gagttttctt
     2401 ggtccgtgcg cgggaatgac caggaccttt tccacatccg cggttcggct tggaccggtc
     2461 accagcacct gatagtcgcc ccacgcgcca gacttgcgtt tcacggagcg agcgaccagt
     2521 gcatgcgcca cggtgggcac gatctgactg ggccgggcca ccacgatatg ggtttccgca
     2581 agcatgatcg gatgaccctg ggatcgatct cgcatatcta ccaacacgcc ccccgtatcc
     2641 gcgatcagca tctcggcgcg cgtgatcccc aacgtatatt ctcgtttttc cgtcgtctct
     2701 ccttggatgg caatcgtatg cgggtcatgc tcttccatgc ggattcgcaa gtattcttcg
     2761 gccaccaatg ctgcatcgaa gagctgaatc tcgctcgtgt tttccggacc gagttcgagc
     2821 atccgttgct gaaagagggg aagcaagtca tcgggtgaat cgacaggctc cggcgcggaa
     2881 gctttttcgg tcatcgccga ttttttccag gactcactgg tgtcggtagc agggcgatcc
     2941 acgtgctggc gctgccacca tcgtcgaaag gattctttgg ccggtggtgg aagtgttcgg
     3001 gattcgagcc atgctttgcc ggtgggatgc aacgcagcga acttgcgaac cagcggataa
     3061 agacgggttc cccgatccat gcggcttgcc ttgtgcatcg cggaagaagc cagacgcagg
     3121 ggccatcgaa gcgaatccgg acctcctttt tgcacgatcc gagcgcgcat cgtgagcaga
     3181 tggtcgtgca aggggatctg cacgggacag gcttgggtac aagcaccgca aagcgaggat
     3241 gcgaacggca ggtctccaaa ccggtccatg ccctcgagat ggggcatcaa tagcgcgcca
     3301 atcggtccgg gatacgccgc accgtaagcg tgtcccccca cgttgcgata caccgggcat
     3361 acgttcatgc aagctccgca gcgaatgcac gtaagaacat cgcgcagttg cgtgtccgcc
     3421 atcagcttgc ttcgaccatg atcgagcaaa accacatgca tgtccggatg cgcatccgca
     3481 agaagagaca cataacagct ctgccgttgt gccgtcgcgg aaacgggaag tagccgcagc
     3541 aataccccca gatctttctc cgtctcgatg agcttgtcga cacccgccaa gacgatatga
     3601 cgtttgggca gagcggtggt taaaaggata tttccctcgt tttcgagcaa gacgaccgta
     3661 ccggtttccg ccacgaggaa gttggcgcca gagataccca catccgcttg aagaaagcgt
     3721 tcacgaaggt gctttcgtgc cgtgagggat agatcccggg caagacgatt gcgggtttgg
     3781 tcatccacga gcgcaggatc gtcgatccaa gcaggcacct gcatgccgag tttgtctcgg
     3841 aaaatgcgag cgatatcctc ggtggaaaga tgaatggcgg gagcggtgac atgcgaagga
     3901 gcttgttcgg caagttggac gatcaactct ccgagatcgg tttcggtgac ttgggcgcca
     3961 gcttgttcga gggcgtgatt gagattgatt tcctccgagg tcatggactt gcttttgatg
     4021 acctttttcc ctgtcgcatt tactaaatca gtaataattt cacatgcttc cgtgggtgtc
     4081 ttggcgaggc tgagtccgat tccgcgggcc ttgagcgccc gcatgaaagt ttcgagcaag
     4141 gcatcatgat ggtcgagggc gcgcagtcga gcctctcggg cgcggtgacg gaggctatcc
     4201 cagtccggca ttgattcgac cagccggtcg cgtccgtggg tggtgtgcag ggtggcttta
     4261 tggactttgt cgccaagccc gggttcgttg agtttggcga ttgaggcttg tcgaaaacca
     4321 tgggcgctca tgttgcctcc aggccggaag ccagcacttc agcgacatgc acgagtttgg
     4381 ggcgagggat tttttgacgg tcgagcagtc caccaaggtg catcgagcaa ctcgagtcgg
     4441 tggagatgac gaaatcgacg tcggcctggc gaatgggccc gagttttcgc tgccccatgg
     4501 ccgtggagat ttcggggaat ttgacggaga aggttccgcc aaaaccgcag caaaacgagt
     4561 cggaaggggt gggtacgaca cacaggcctt ggaccgagcg catcagcgta tgcacggcgt
     4621 tttcgatccg cagttcccga agggcgtgac atccgatatg cagggcggcg cgaccctcga
     4681 aacaagcgcc caggtccgtg acgcccactt gattcacgag gaattcgcag agttcgaaaa
     4741 cccggtggct cacgggagaa tccacgagct gtgggtaatg gtggcgcacc atggcggtgc
     4801 aagaaccgga cgggcaaacg atggcatcga aggggcgaaa cacctcgcag aaatgccggg
     4861 ccaattcgcg agcttgttct cgatgacccg cattgaaatg aggttggccg cagcaagttt
     4921 gttgttcggg atactcgggc cgcagcccga gatgctcgag aatgtggacc gtggcgatgg
     4981 ccacggaggg atagaagctg tcgacgaaac aggggatgaa aaggccgagt cgcacgaggg
     5041 cagttgtcgt tggtgtgagg ggagatgtca aacggccggt gcgtggtttg cgtcaagcgc
     5101 gcgaaagtac cacattcttc cgactccata gcgtttggca tcccggtggg gatccacctc
     5161 gtggcgtcgg ctgtcataag cgagcatgca ttgttcgaag tcgatcccct ctcgcatttg
     5221 catggcttgc ccaccggggc cggccacgag agtaagtccg acgacccacg cttctccgcc
     5281 gagttttccg gtgctgtcat cgatgaacca ggcgccgcaa tggctacagg ttccgacgag
     5341 tgtcgaggaa aaacggcaat ccggcttcgg tcgaggtatt tttttctggc aaaacccgca
     5401 atgagccggc tgcggttggg aacgtttcgg aatcttgttc ggatcgattt tttttctggg
     5461 gcctagcttc aacatttcgt tcggtatcgg agaattgcgg atgaccgatg gccgtcggga
     5521 tgagattacc acgcaaggat gcagccaacg cttgacattg cacgtgcgat tgataaataa
     5581 cgcggcccgg ctctgctgag tctggcgagg tagccaagtg gttaggcaac ggtttgcaaa
     5641 accgtcaaac gccggttcga atccggtcct cgcctccatc gtattcgagg ggtttttcgt
     5701 atcacgcggt gatgtgccgt ggtgtaggaa atggacgtcg tcgcgcaaaa acgcgcggtg
     5761 agcaaagcac tttgcctcat cccgcatcat tgtgagaaga tgtctcctcg tatggatgca
     5821 ccgctgccac tcgccgggcg ttaccgctac ctgcgggggc tacgaggagc ctcctcgggt
     5881 ctgatatggc tcgcgtcgga ttccgtcgcg gggcgcacag tcgtggtgtc ttccgtgaca
     5941 cccattcgcg tggcgggttt gaaaaacgga ttgggactga cccaccttca tcttgccacg
     6001 ctgctcgacc tattcgatga catctctccc ggccaaatcc caggtcgtgt ggcattacga
     6061 cccggtatgg ccatcgcggt agccgaacat atcgcgggtc aaacactgca tgagcggttg
     6121 gaatccaccc cgctgtccct gcatcgcgcc gtaggtgtgg tggccaatat cgccaatgcc
     6181 ctgcaggcgc tgcatgccaa aggaagcgtt catggagccc tgtctcctcg aagcatggtc
     6241 gtgttgcgta acgatggcgg tgtcgtgccc gtgctcacca acctgcgggc cgccgccaac
     6301 ggcgcctact gctcgccaga acgtgtccga ggaggtggac cgagcaccga tgacgatgtt
     6361 tgggcgcttt attccacctt atttgccgct atgaccggcg atgccccctt cgagggaaaa
     6421 actcgcgaag aactggccgc ttccatcttg tccggcaccg tccccaactt ggtcgtccac
     6481 ggtgtcgatg atcccgacct gcaagaaatc attcgactcg ggttccatcc ggatcgcaac
     6541 gcaaggcaaa cctccgccgc ggttctcgaa aatgtcttga ccgcatggct gcgtgaacat
     6601 ccctccgaac atcaagaacg cgtctccatc tccgcgccct tcaccagctt gccgcccccg
     6661 cccatcgatg acgaagacgt cgcggaagga catcgtacgc ttgccgttgc ctcgatcttt
     6721 tcttcaggaa ataacgccag ttcgtcaaac gcggacaacg acgatgacga tgatcaagcg
     6781 acgattctgc tcgatgcgaa agccaatgtg gccgccgcga ttgccacatt cggttctccc
     6841 ctcggtcatc ccgacgatga tgcgacgttg caagatcagc ttcccaccaa cgtgatgtcc
     6901 actcccgaac tcggcgcgat gctcgagcgg atcgaagccg aatcggcacg cgcgcccatg
     6961 ccgagtgtga cccgttccat ggatccacaa gtcatcgatt cgtttcgagc gatgtccagg
     7021 gaacaaagcg atcctcaggg agcagacgaa aacagtaatc taaacgcagc tttaccaccg
     7081 cctgctgcga tcgctccacc gcttgacccg gtcgctccac cgtctgacgc gatcgccatg
     7141 gacgatcagg tccttgccgc cgcgcccgct ttccccgttt ctggcccaca aaaagggtgg
     7201 ggaccgtccg agcctacccc gcctgtcatc gatccggaaa gcttgccctt ggcaacggag
     7261 ccccttgtgc cggaacagga ctggatcgat cccgctgccg taagggcctc actctccgac
     7321 ccagaaccca cttccaagaa aagcagtgcc ttgcgatggg tgttcggagg tttgctgctg
     7381 atggtcatcg cggccaccct ctctttcatc cttgctgtcg tggtttccgg aggaaccctg
     7441 cccgctccca tctcctcgtt gccacgtgtg ttccgatcgc tcttccaccc cgctgacgac
     7501 acctcccacg ctgccaccaa tccggcatcg aacgaaatcc ctccagcgcc ggcatcaagc
     7561 tcgtccgcgc cggagcaggc ttccgccagc gcttctgccg aaatgatggc ttctgccagc
     7621 gctcccccca ctgccctttc cgaagagcaa ctgaatgcgt gtgcgcagga cttctttccc
     7681 ccaaatacct tcatccgcac cgtttccttc gacttccttt gcgccaacga taacccacgc
     7741 aaatccgcgg tgatgctgca ccaacaaatc attcatgcag gggccggcag cgtcaccgac
     7801 ggcatgcgcc tgtggtcctc gctgagttgg tacgaactcg tcgtcgtgac catgattcgt
     7861 aacgcttgct gtccaggcgc cgcacccctc gtattgccag aacccggaga accctgcgct
     7921 tccatggtcg atgtccttca ggacgccagt cgtggaccat gcacacaacc acatctcgga
     7981 cagcgaattt cccgcttcga ggaatccgtc cgatgcttgt acgcccatga tcgtcctcga
     8041 ccttatcggt accaaggctt gctcaattcg catcagcgcg cgaccttcga agaattcgcg
     8101 gggagaatac cgcccgctcg ctgcgctccc ccatgaggcg atcatcacgc gtccgaacgc
     8161 ttggtgttca tataaaatca atataaattt cagttggtta caacaagttc atcgcatggt
     8221 gccgcgaacg aaacccacgc cgaattgcca ttcaggagaa cgccatgagc tggacgtggt
     8281 tcatctactc cacccggtct atgaaagaga ctctcgccct catcgatgat gccaacgatg
     8341 tcctcgacgc ctgggcacga gcgcgtgggc ccgaaggcga cgaagagcga atcggtacgt
     8401 gcggcaccat cgaaccagga ggccccattc ccaccacgac gcagatgcgt ggcattcttt
     8461 ccccgcgcgg tcacgctgcc gatcccattg tcgaacgact tcgatcgtgt cgctcgtcca
     8521 ttgctctcga ccgaattcgt ggcaccggct tggaacatcc cctgcaagta tccgtcgttg
     8581 gctatctgct gcaacgcgcc ggtccatccg tggtcgattg gggcgactac caactcgtcc
     8641 tcggagaaca ggctctcgct tatgtacttc aactgcctaa ccatgggcca ctcgatgagc
     8701 ggcctccacc ttccgtccat ccctcctccc cgctgcagaa ccccctgcaa cagcgcgcca
     8761 tcgccttgct cgatgcactc gaacaagcgc atgccgatgt cgatcgggcc atcgacttcg
     8821 accgtctcgc gcgctcgttt tccgatatcc aatcgcgtta catcaggttt ctgctcgaag
     8881 agggcgccgt agacgacgct tccgctgctc gccatctcgg catcagcgaa tccgtcctcg
     8941 accaacaagc cgatgcgctg ctgatcgctt tacacaacct gatcgaccct tgagtctgcc
     9001 atctccgtct ttctacccat cgcatcttcc cccgtgatgg atgtggtgat tgcgaattac
     9061 gctgaagtgt cctgaaggat cccttgtcgc agttccagcc acgagtcgtg ttggcgctgt
     9121 ctaataaaaa tatcgtaaat acaatcgttt acctctgaca tatgccgcag gatcgctttg
     9181 gcgcgacccg ccaccgtccg ttcccccagc cccttggcca cgcacgcatc accgtaggaa
     9241 acgagccagg ccaggcgatg tcgaggctgc cagggcactt ccgacaatcc tttgagccac
     9301 cgaaataact caggcttcgc aagggccccc cggccgatca tgaaacgttc gcagccggtt
     9361 tgctccgcac atcgggtcat atcttccacg cttcgaatat ccccattcgc caccacggga
     9421 atcgtcaccg cgcgacgcgc tttgccgatc gcttcccaat ccgcttttcc ctcgtagagc
     9481 tgttttcgcg tacgcccatg catggtgagc catcccgcgc cgccttgctc cacggcctgc
     9541 gcaatcgcgg gggcttcgtc ggtacgagac cagccggccc gcaccttggc cgatagggca
     9601 atggacgacg gtaccgcatc tcgcaccgcg cgaatgacgc gtgtgatgcg acgaggctct
     9661 tcgagcaacg ccgcgccccc atcgtgccga ttgaccctcg atacggggca accgaaattg
     9721 agatcgattc ccatggcacc cgcatccacc gcgcgccggg cggtttcggc catgagcgat
     9781 ggatcgcttc cgagcaattg gagcacgacc gcggtaccgt ggcgtgtgcg tcctcccgtg
     9841 gccatttcgg gacagatgcg aagcaacgaa tgcgcgggca gaggcaaatg gctgacccgc
     9901 gcgtacacgc tcacgcaagt atcgatacct ccgagttggg tcaatgcctc gcgaaatatc
     9961 gaatcggtaa ttccctgcat gggggcaaga agcaggatgg gagctgtcat gggagcgcgc
    10021 ttttcaacat catcgacaat gggaaatggg ctgcttgcga aggaccatga ccctgtcgaa
    10081 atcgtctatc ctcgggtcgt aacgttgggg cggccttggt gccttgggta tcatggaacc
    10141 tcgcggattc gagcggtgag gaaaaaatcg aagcgtgagg cgtggccatc cctagggtgg
    10201 aatgggataa ttaatatgta attacaatgg gctacgtgat tagtggcacc tgttccgacg
    10261 agtcttcgga ggtgacagct acgacttccg cgctcaatcg ccctttggat agccgcaccg
    10321 tgaccatatc cgcaacagca acgtgggagg catccatcaa cgtttggccg tcgggccgag
    10381 ccacgatggc ataaccgcgg gccaagacgg cgaggggtga caaggcttcg agccgggcgc
    10441 tggttttgga taacgcggct tgttgggatt gcatcttggc ctgcatcgcc gaagcgatgc
    10501 gaagccgcag cgaatcgaga tcgcctcgcg tctttgccaa taccgtcgac ggatgccgcg
    10561 cgatcagcct cttttcccat tgctctagcc tgctacgact cgagatctgc ttggcccgaa
    10621 gggccccttg cgcgcgttgt atcaggtcat cgagtccctg tgcacgttca tccaacgagc
    10681 gtttgggatc gcccagccga gtttgcagct tttcgaatgc tatcctgtcc tcacgaaggt
    10741 gtgtcgtcat cgcacgctgc attcgtgttc gaagatgggt aagcgagcgt gcgctttgct
    10801 gtcggtccgc aaccaccatt tccgcggctt gcgatggtgt ggcggctcgt gcatccgcta
    10861 cccaatccaa tagggtgaca tccaattcgt gtccgacggc ggatatggtg gggattggac
    10921 aggcagcgac gaaccgtacc aggcgttcat cgttgaacgc catcaagtct tccagcgagc
    10981 cgcctcctcg gcccacgatg atcacgtcga gtccttgcac gcgttgcagc atgccgagcg
    11041 cggccatgag actgcgtggc gcctcttcgc cttgcacttg tgcgggagct agcaggatat
    11101 tgggggagcc ccggcggaac gcgacacgaa tgatatcgtg gatgacggcg cctgaagcgc
    11161 tcgtgaccac accgatgcgg tgtgcatcgg tggggatggg gcgtttgcga gcagggtcga
    11221 atagcccttc tgctcgcaat ttttctttga gtcgctccaa cgcttcgagc agcgcgcctt
    11281 ttcccacggg acgcgcggct tccacgacga actgaagttt gccccgaggc gcccataccg
    11341 tcgcttttcc acgagccatg attcgcgtcc cgtcgaccag gacacgacgg actcgcaggg
    11401 cgtcatcgcg ataggccacg gcttcgatca tcgcgtcttg ttgctcgtct ttgagcgtga
    11461 agtacatatg gcccgaacta gctgttttta cggcctggac ttccccctgg acccaaacgc
    11521 ccccactcac tttttccgcg gcgaaccgaa gtcttcgatc cagttccgcc acggaccaca
    11581 cggtgggttc ggacgacggg gaagggattt caggcggggt agccatggcc ctgcagccta
    11641 aagcagtttt ttatgcggtg gaaccggttg tgcatggcga caggccgcaa gacctggcag
    11701 ccgcgaccgg cggcgcgtct ttgttcacca acacttggga ccgaatccaa accgaggtaa
    11761 gaaacccgcc atgttcaaca aggtgcttgt cgccaatcgg ggcgaaatcg cttgccgggt
    11821 gatccgaacc ctacgagaca tgggaattcg ctctgtcgcc gtgtatagcg atgtggatcg
    11881 tgccgcattg cacgtaagaa tggccgatga agccgtatcc gtgggagagg cgcccgcgtc
    11941 atcgagctat cttcgcgtgg atcgaatcct cgaagccgtc caaaaaactc gtgcgcaagc
    12001 ggtgcatcct gggtacggtt ttctttcgga aaacgcggct tttgctcagg cgtgtgagga
    12061 taacagcatc gttttcatcg ggcctccggc ttccgcgatt gccgccatgg gttcgaaaac
    12121 cgcggcgcga gaactcatgg aaaaagcagg cgtgcccgtg gttcccggcg cccgttgcgc
    12181 caccgtgcaa gaagccaccg aacacgcgca acagctcgga tatcccgtca tgctcaaagc
    12241 ggccgccggc ggtggcggca aaggcatgcg cctcgtagct tccgaagacg agatggcaaa
    12301 cgcttgggaa cgcgcaaaaa gcgaatcaaa aaaagccttt ggcgacgata cggtctacct
    12361 cgaaaaggcc atcgtcaaac ctcgccacgt cgaaatccaa gtcctcggtg accgcttcgg
    12421 caacgtcgtt catcttttcg aacgcgattg ctccatccaa cgacgacacc aaaaagtcgt
    12481 cgaggaaacc ccttgccccg tgctgacgca ggatatcgtc gaaaagatgg gaaaagtagc
    12541 cgtgcagggc gccaaagccg tgaactattt ttccgccggc acgttcgagt ttctcctatc
    12601 cgcggaccag tctttctact tcctcgaaat gaatacgcgt ctgcaggtcg agcatccgat
    12661 taccgaatgg gtcacgggta ccgacctggt tcgagaaatg attcgtgtgg cggctggaga
    12721 gcccctgtca tggaatcagg aacaaattca acgacatggc gcatccatcg aatgtcgcgt
    12781 ctacgccgaa gaccccgcca acgacttctt gcccagcccc ggcaccatcg aacaactccg
    12841 cgctccagca ggacccggcg tgcgtgacga tagcggcgct tatcctggtt gcaccatctc
    12901 cagcgattac gaccctctca tttccaaact cgccgtctgg gctcccaccc gagagcaagc
    12961 catcgcacgt atgcgacgcg ccctacacga atatgtgatc acgggaatcc gaacgaacct
    13021 cgccttccat gaacgattga tgaatcatcc gggctttatc cagggggttt atgatacggg
    13081 attcatcgat gcccatcgcg atcaactcat gggattcgat gtgccccacg aagagatgac
    13141 cgaaccgctt gctgtggctg tcgccatggc ggcggtcgga ctcgaacgaa agatggcggc
    13201 cacggctcac gcggattgtc tcgatgcatc ccgactctcc gcgtgggtag ccacccatcg
    13261 agcgcgtcga tcgacctgac gattcaggtc acagcatcct cagattccac cttccggcaa
    13321 tgcgcaatcg tccccacagg ctgccgaaat gcattttccg gcctccatgg caatgcctac
    13381 cgatgggtcc gctgggctcg tcaccccatg cttcgaagca catccccatc cgcagccaat
    13441 atcgattccg ttcggccctc caaaacactg atcctcaaac aaacaaagca gtatcgaccg
    13501 acatttgtcg tcgtcctcac acgcttgcat ctccgtggtg cagtggtcca ccgcgcagtc
    13561 gtaacacccc tgcaacgtcg cacccgactc gttgagcgct acatccggca tcgtgagatc
    13621 gaatagacta ccgtcgctga ccggtgcgtc aggcacggac gtctcagggg tcacggattg
    13681 gtcggcggat acatcggaaa cggtatccgt agtcacgtcc tgacctgttt cggg
//
LOCUS       DAOSOR010000082        13687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4511_length_13687_cov_4.740830, whole genome shotgun sequence.
ACCESSION   DAOSOR010000082 DAOSOR010000000
VERSION     DAOSOR010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 13687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13687
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4511_length_13687_cov_4.740830"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="PK710_06485"
     CDS             <1..1235
                     /locus_tag="PK710_06485"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOT09396.1"
                     /translation="REYPPPRQVKPGYPPRLDAIVTKALAKDRTARYQSARQMQADLE
                     AYIREERVAVSPLGLRDWMQKLYGDKLTEQQDTLHDLKQLADVIAEQHAIEGTDDFHD
                     SSSGATGFSMAPGPTPAVERSPIPTKRSPLVAVLLAVVLGGALSLGLFLLLRTPSVSP
                     PTVAMAPTASQVPVPSYGTVRLITRPSGASIWIDGSLRSEKTPATIDKLPQNTPISIR
                     LTMEGYAPVRQEVTLTNDSPEAIERDLVAGTVTVAVTTQAPGAKLFIDDKVYDGLQAE
                     GLSADEEHKIVLAANGYQSRTMRFRAGPNERKEFTLNLEREGVTKPKAAVTADTPAPK
                     PEPTHQGQGKLNVSSRGGYCNVFIAGRSHGPTPVGGIVLPAGNHVVTCKPTEGAAKSM
                     GVTITADQTSRITFQLDG"
     gene            1545..2279
                     /locus_tag="PK710_06490"
     CDS             1545..2279
                     /locus_tag="PK710_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09397.1"
                     /translation="MRVMTWLLGLTLLVGCCKEKQKPDPLDMDPVSVSPANPTTTLPS
                     PEPVPAQPAMVWEESTIKTIPDHCSDAKAVLAVITHEAYSKPGFEWKWVRQVMLANPQ
                     FTVVPHAALMPGMVTFQDYDYGTSNAKALVAHCGHGGTCNQVAKAYKRIVRSSKPTVY
                     CGPVPGLGKPVSAVPLWLDGGPKANLPQSGDVISQCARLAACALVKDQTIPGDPGLEC
                     QRAPSRFALACASKASCAEVNACAGR"
     gene            complement(2326..3210)
                     /gene="rfbD"
                     /locus_tag="PK710_06495"
     CDS             complement(2326..3210)
                     /gene="rfbD"
                     /locus_tag="PK710_06495"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOT09398.1"
                     /translation="MRVLVLGAGGQVGFECAAVLRLTAEVLALTRGQLDITDSDALIA
                     MLQNWQPDVVVNGAAYTNVQEAEAKEAQAHAVNARAVGILGEQAKKRRFGLIHFSTDY
                     VFDGRKDGAYEEGDTPCPLNAYGRTKLEGERALIEADAPALILRTAWVYGMRTHNFVS
                     TILRLARSRPVVSVVTDQRGNPTFCRDLAEAVALLSSRLQGQRGGFEAWRAWRGVYHL
                     AGQGMCSRFELAQAVLSLDPRRSEHLVRHIVPITSPELASSVCRPERVNLNTNLAFER
                     LGIRLPPWRMSLQRALAG"
     gene            complement(3213..5153)
                     /locus_tag="PK710_06500"
     CDS             complement(3213..5153)
                     /locus_tag="PK710_06500"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOT09399.1"
                     /translation="MMQRFAHRSFLIVIDLAVLAIAYSLAFLIRFDGQIPLLMTKKLL
                     FTLPYIVIFQFLVLLFSGVTRFAWRYISLREVIRFGLAFGVANGFLLFVRHGSIEIKP
                     HFGYAEYAVIPMGVIFIDAFLAFTGAVGIRGLRRIVAERFSHPLLSKKDKQVRTLLLG
                     AGAAGALVAREIANRTELGIKPVGFLDDDTTKTGLVIHGIPVLGTTAALGPIAKKQAV
                     DQALITIASAPGEAIRRIVHVCDEANLPVRIIPGVFEILDGTVNLSRIREVSIEDLLG
                     RAPIDLDAPLIESFVRGKRVLVTGAGGSIGSELCRQLARFHPASLTLVERAEFHLFTI
                     HQDLIHAFPDLDIHPRICDVCDSRRLEAVFSQDRPHVVFHAAAHKHVPMMEWNPGEAL
                     KNNVFGTQKVADAADRHNCDAFVLISTDKAVNPTSIMGASKRVAEMYVQSLSQRSKTK
                     YVAVRFGNVLGSAGSVIPIFQKQIEAGGPVTVTHPEMKRYFMTIPEACQLVMQASAMG
                     DGGEIFVLDMGTPVKIADLARDLIRLSGFEPDVDIPIVYTGVRPGEKLFEELGFDAEK
                     MNTTRHPKIFVGKLKGCALTQVTRNLEELAQFTSSTSAQQVRQALGNMIPEMQPDATE
                     RPPSVVPDRHSTPTPSLPLVAV"
     gene            5342..6481
                     /locus_tag="PK710_06505"
     CDS             5342..6481
                     /locus_tag="PK710_06505"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT09400.1"
                     /translation="MTRVYLSPPHMGGEELRLVEEAFETNWIAPLGPHVDAFEQEMAL
                     KIGVPHACALSSGTAGLHLALLMLGIGRDDEVVTSTLTFSATANAITYVGAQPVFVDC
                     HASTWNMDPELLAEYLERRAGQGKLPKAVAVVDLYGQCADYGPLQATCARYAIPLLED
                     AAESLGATYQGRSAGSFGEMAVFSFNGNKMITTAGGGMLVSHNKTYTDRARFLATQAR
                     DPAPHYQHSTIGYNYRLSNVLAAIGRGQLRVLDERVAARRANFSFYQSALGDLPGLTF
                     MPEAPYGMSTRWLTCILIDPAAFGASREDIRLALEKQDIESRPVWKPMHLQPVFQGCA
                     TVGGDVAASLFESGLCLTSGSSLSLSDKQRVVSIIHEVHDAARRG"
     gene            complement(6503..8467)
                     /locus_tag="PK710_06510"
     CDS             complement(6503..8467)
                     /locus_tag="PK710_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOT09401.1"
                     /translation="MDHLLGTTVLSGHRLDSLVAVDDAVATFRALAPDGSIGTLRISL
                     LIEVSDAQLESLPTELTRLARHAVGIQALLAPRAAGRVIVDNVPRLAMVHAGDPCPNL
                     ESRLQEGKPASIDSVLGLLEPIARALQALHDQGMVHGAVQPSALRFDVLGSDLSAFGW
                     SDLAMGLQGPPGACHALSVSRRPPELSLPHPTAPTPETDTYALAWIVLELLLGKRVGS
                     QDQTVLPEAKGHGLAPSIEAVLSEALHPQAQQRRLDPQTLLRKLSEARFEANRPAPAP
                     ADDPIPAPFTQPSKPEPAEVAPTPELSLFGQRRVTSTQEQTRVTAPTPAPLFRFDAPA
                     PKRADAWFVYALVGLGALLLAGGVGSVFYYMSNASPKVAESTTSPSSSPPVLTVPTAA
                     PTPPPPSSPPVLTAPTAAPTPPLVLPDAPQPLAQDSQEDASVPKLPAGPPSSEALAVY
                     PEDASALVPVLADTVVFGTRDAPVTVVLFVDMRCPHSGSAQKAMSQLLAEYPSDVRLS
                     IRHLPVDGAASETAAQIAVGSWALSGRKVFWDVFGTLTRHGDEREQDELLQWAEQAGA
                     DKQSLADTLRKGTYRSVVERDVNLAGQLMVRATPTFFINGIRLNGMPPQVKLQKVVQA
                     ERFAMMRSTPGGSSRYASRVLFNITAATDD"
     gene            complement(8580..9509)
                     /gene="hemC"
                     /locus_tag="PK710_06515"
     CDS             complement(8580..9509)
                     /gene="hemC"
                     /locus_tag="PK710_06515"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOT09402.1"
                     /translation="MIPTRLIMATRRSMLALAQSRAFASRLAQAHDLSIDELHVITTG
                     DRVQDRPLTEIGGKGLFVKEIEQALIDGKAHFAVHSIKDVPGELAPGLVLACIPKRED
                     PRDVFVSRYGHDLSQIEHAARVGTSSARRRIMLAKARPDLRFEPLRGNVDTRLRKVQE
                     GVVDATVLAWAGLRRLQLHDRATQVLPPELSLPAVGQGALGIECRSDDASTMALLSTL
                     HDSETATAVAAERGVMIAAEGSCQVPIAAYACRDEEGKIWLRAMLATPDASRVRWMEI
                     REPWPATEQQAGTLGKQLGAELRIALEEPLVSH"
     gene            complement(9506..10900)
                     /gene="hemA"
                     /locus_tag="PK710_06520"
     CDS             complement(9506..10900)
                     /gene="hemA"
                     /locus_tag="PK710_06520"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HOT09403.1"
                     /translation="MIVVVGASHKTAPIGVRERIAISADRLPTLLSSLHELPEIAEIC
                     IVSTCNRVEIYAATGRHEDDAAQQTIATIEQALAEQAGPKLGDVLHQYLFHYIGDAAV
                     RHLFRVSSSLDSLVVGEAQILGQVKDAFDLATAQGTLGPLLGRAIEWAFHVAKRVRSE
                     TQVGAGAVSVSSVAVELAKQIFGGLNRRVVTLIGAGTMGEAAARHLCDAGARLVVVNR
                     SLERAQIVAEHFHGSARQWNQLQDALLETDVVIASTGSQDYLLTPQWLAPIQRKRKGR
                     SLFVIDIAVPRNVDPRSNQIDGVYLYDIDDLSKMAATTLRDRFKEAQISERIVAEEAD
                     AFYVWQDSLQVTPTIVALRNQIRAVLDAELDKSLHGKLKHLGEAERKALEGMLSAAVK
                     KLTHQPTIRLKSAAAEGNARTLVDAIQELFGIDPHEIPASSRSPSLPPTAHSDTAPIS
                     RSLEYRDAHPEEAS"
     gene            complement(10897..11712)
                     /gene="ccsA"
                     /locus_tag="PK710_06525"
     CDS             complement(10897..11712)
                     /gene="ccsA"
                     /locus_tag="PK710_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOT09404.1"
                     /translation="MNHALLPDIVLGLAALGYVAATIAFAIHLARQGGWEKALRVARW
                     ILAGSVSLHALALGLAGIAQPVCPMKTIHFGVSMGGLVAAFVYLIARRVLRIYGLGVF
                     VAPVAVVFLLAGRFMAEPAVVPALHTGMLPVHIAANILGDSFFVLAAGSATMYLVQER
                     QLKTKRGASVFGRLPPIDSLDRASHIFVIVGFLLASFGAATGTIWIAKLSLSTTTEIV
                     RLLISYASWAVFSAVLLLRATLGWRGRRAAYGTLVGFVFAMIVLTLYLFREGS"
     gene            complement(11719..12249)
                     /locus_tag="PK710_06530"
     CDS             complement(11719..12249)
                     /locus_tag="PK710_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09405.1"
                     /translation="MSTVDLSDSNQSVPNRLALVSKKAPTTTCSKAQPGPSCTPCRAT
                     MAMMMGKNRVFFPQEALDAWLVSGAIDLQGQNLLLKDEGRRYALTEGARILTEVTGET
                     DAFDIVGKCKTLAFLHELGADILERSMIVDNNAYDIVPGFLGTPIGAEDEKESEARST
                     LLSDEDLLAQFLAKNM"
     gene            12282..13610
                     /locus_tag="PK710_06535"
     CDS             12282..13610
                     /locus_tag="PK710_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOT09406.1"
                     /translation="MTLKLALAALCVIANAFFVAAEFALVKVRVTQLETLAKKGEKRA
                     AVAMKVTGKLDAYLSATQLGITLASLGLGWLGEPTVAVLIAPVLRFFGIESEPLVSAV
                     SLTTAFSTITILHIVVGELAPKSLAIQQAEWVALRVATPMRAFFYLTYPFLFILNELS
                     NLTLRLFRITPVQHAEGALSAEEIRLVVAAGQLDPKKRELMERALQGTDRPVRSIMVP
                     RVDMEYISLLDSPEQIIASLRVNGYSRVPLIEANDPDKIVGYVYLKDIFVGDGLPAGG
                     IRALRRDILFVPENRTVGDVLEDFKRTHIPIAIVVDEYGGTSGLVTMEDILEEIVGEL
                     QDELDVEAPPIVRRDDGTIIVEGDVPIRELEALGIVVDPIEGHEIVAGHIISRLGRLA
                     RPGDIVSLGSHDATVEDVRSRRITRVRLAPKPEELPKVPSQAPGPVDDAS"
BASE COUNT         2990 a         4079 c         3812 g         2806 t
ORIGIN      
        1 atcgcgaata cccacccccc aggcaagtca aaccaggtta cccccctcgt ctcgatgcca
       61 tcgtcaccaa agcgctcgcc aaagaccgaa ccgctcgata ccaaagcgcg cgtcagatgc
      121 aagcggacct cgaagcgtat atccgagagg aaagagttgc cgtctccccg ctgggattgc
      181 gggattggat gcaaaaactc tacggagaca agcttaccga gcagcaagat accttgcacg
      241 atctgaagca actcgccgat gtcattgccg agcaacacgc catcgaaggc acggacgatt
      301 ttcatgattc atcgtcggga gccacaggtt tttccatggc gcctgggcct acgcccgccg
      361 tggagcgaag tcccataccg accaaacgca gcccccttgt agccgtccta cttgccgtgg
      421 ttttgggggg agccctgtcg ctgggattgt tcctgctgct gcgtacgcct tcggtttctc
      481 caccgacggt tgcgatggca cctacggcct cacaagtccc agttcctagt tacggcacgg
      541 tccgcctgat cacacgacca agcggtgcct ccatctggat cgacggctcc cttcgttccg
      601 aaaaaacgcc cgctaccatc gataaactac cgcaaaatac gccgatctcc attcgcctca
      661 ccatggaagg ctacgccccc gtacgtcaag aagtgacgct caccaacgat tcacctgaag
      721 cgatcgaacg agatctcgtc gccggcaccg tgaccgtcgc cgtcaccacc caagcgcccg
      781 gcgccaaact gttcatcgat gacaaagtgt acgatgggct gcaagccgaa ggactatccg
      841 ccgatgagga gcataaaatc gtgctggcgg cgaacggata tcaatcccgc acgatgcggt
      901 ttcgcgccgg tcccaatgaa cgtaaagaat tcacgctcaa cctggaacgg gaaggcgtaa
      961 ccaaacccaa agccgccgtg actgccgata ctccagcacc caaacccgag cccacccacc
     1021 agggacaagg caaactcaac gtcagttcac ggggcgggta ttgcaacgtc ttcatcgctg
     1081 gccgatccca tggcccaacc cccgtcggag gaatcgttct tcccgcggga aatcacgtgg
     1141 ttacgtgcaa accgacagaa ggtgccgcca agtccatggg ggtaaccatc accgcagatc
     1201 aaacatcgag gatcacgttt caactcgatg gttgaacccg acgatcccgc tacctcgcta
     1261 cccgtggatc acgctgtctc cggggcccat catctacttc gacgccgctc actgtctcga
     1321 tcgcccttgc ctcgaatcag aacgcctcgc acccctgtct tggtttcatc gtatttgttc
     1381 ctgttatctc cgtatcctca ccgaagtcct gactctccga acatcacaaa tcatccaacc
     1441 gtcccacgaa ccaacgcgtc tgtgtcgtcg tctgttgtat ctttgcctct tggatggatg
     1501 tggtttgcct taacccatga gcgtctgtcc aaggagcgtc agatatgcgt gtcatgacat
     1561 ggctgttggg tctgactctg ctcgtaggat gctgcaaaga aaagcaaaaa cccgatccgt
     1621 tggatatgga ccccgtttct gtctcccccg ccaatccaac cacgacatta ccttccccag
     1681 aacccgttcc ggcacaaccc gcaatggtct gggaagaatc gaccatcaaa accatcccag
     1741 accactgcag cgatgcgaaa gcggtgctcg cggtcatcac ccacgaagcc tattcgaaac
     1801 ctggtttcga atggaaatgg gtgaggcaag tcatgctcgc caatccccag ttcacggtag
     1861 tgcctcatgc ggccttgatg ccaggcatgg tcacgtttca agactacgac tacggtacct
     1921 ccaacgccaa ggctctcgtg gctcattgcg gtcatggcgg cacctgcaat caagtggcca
     1981 aagcctacaa acgaatcgtc cgatcgagca aacctaccgt gtattgcggc ccggttcctg
     2041 gcctcggaaa gccagtgtcc gctgtgcctt tgtggctcga tggtggaccc aaagccaact
     2101 tgccccagtc cggcgacgtg atcagtcagt gtgctcgcct tgctgcttgc gccttggtca
     2161 aagatcaaac gattccggga gatccaggat tggaatgcca gcgcgctcca tcgcgttttg
     2221 ctttggcgtg cgcaagcaaa gccagttgcg cagaggtcaa cgcttgcgcc ggacgatgaa
     2281 gcacacctga aacgcccgca tcccattcac ttcgaaatgg gctcgtcacc cggccagagc
     2341 acgctgcaga ctcatccgcc acggtggcaa ccgtattcca agtcgctcga acgcaaggtt
     2401 cgtgttcaag ttcactcgtt cgggtcgaca aaccgacgat gccagttcag gtgatgtgat
     2461 agggacaatg tgtcgaacca gatgctcgct acgtcgaggg tcgagcgaca agaccgcctg
     2521 cgcgagttcg aaacgagagc acattccttg tcctgccaga tgatacacac cacgccatgc
     2581 gcgccatgct tcgaacccac cgcgctgccc ctgaagtcgg ctcgacaaca aggccacggc
     2641 ctctgccaaa tccctgcaaa acgtaggatt tccccgctga tccgtgacca ccgaaaccac
     2701 gggccgtgag cgtgccaggc gtaggatcgt agacacgaag ttatgcgtac gcattccata
     2761 aacccacgcc gttctcagta tcaacgcggg agcatccgcc tcgattagcg ctctctcccc
     2821 ctcgagtttc gttcgaccat aagcattcaa aggacaagga gtatcccctt cctcatacgc
     2881 tccgtccttg cgtccatcga acacataatc cgtggagaag tgaatcaatc cgaaacgcct
     2941 ctttttcgct tgttcaccta gtattccgac agctcgtgca ttgacagcat gcgcctgggc
     3001 ttccttggct tccgcctcct gtacgttggt gtaggctgca ccgttgacaa cgacatccgg
     3061 ctgccagttt tgcaacatcg cgatcagggc atccgaatcg gtgatatcga gctgcccacg
     3121 cgtcaaagcc agcacctctg ccgtcaatcg cagaaccgcg gcgcattcga aacccacctg
     3181 ccccccggcg ccgaggacga gcactcgcat cgtcatacgg ccacgagagg caacgatgga
     3241 gtgggagtgg aatgtcgatc ggggacaacg ctcggcgggc gctcggtggc atccggttgc
     3301 atttccggga tcatgttgcc aagggcttgc cggacttgct gagcgctcgt ggatgaggtg
     3361 aattgggcaa gttcctcgag gtttcgcgtg acctgggtca gagcacatcc tttgagtttg
     3421 cccacgaaga ttttgggatg acgggtggtg ttcatcttct ccgcatcgaa gccaagttct
     3481 tcaaagagct tttctccagg acgaaccccc gtgtatacga tggggatatc cacatcgggt
     3541 tcgaatccgc tcagacggat gagatctcgt gcgagatcgg cgatcttgac gggcgttccc
     3601 atgtccagca cgaagatttc tccgccatca cccatggcgg atgcttgcat gacgagttgg
     3661 caggcttctg ggatggtcat gaagtaacgc ttcatttccg ggtgggtcac ggtcaccgga
     3721 ccgcctgctt cgatttgttt ttgaaagatg gggatgaccg agcccgcgga cccgagaaca
     3781 ttgccgaatc ggaccgcgac atacttggtt ttggatcgtt gggacagaga ttggacatac
     3841 atctccgcca cccgtttgga tgcacccatg atcgaagtgg gattgaccgc cttgtcggtg
     3901 ctgatcaaca cgaacgcatc acagttgtgc cgatcggccg cgtcggccac cttctgcgtt
     3961 ccaaacacgt tgtttttcag cgcctcgcca ggattccact ccatcatcgg cacatgcttg
     4021 tgagccgccg cgtgaaacac cacatgcgga cgatcttggg aaaacacggc ctcgagacga
     4081 cgagagtcac aaacgtcgca aatgcgagga tggatgtcga ggtccgggaa cgcatggatg
     4141 aggtcttgat ggatcgtaaa taggtgaaat tccgcgcgtt cgacgagcgt cagggaagcg
     4201 ggatgaaatc gtgcgagttg acggcataac tccgaaccaa tggatccgcc cgcacccgtt
     4261 accagcaccc gttttccacg aacgaaggat tcgatgaggg gcgcatccaa atcgatgggg
     4321 gcccgcccga gcaaatcttc gatcgatacc tcccgaatgc gcgaaagatt cacggtgcca
     4381 tcgaggatct cgaatactcc cggaatgatg cgaacaggaa gattcgcttc gtcacaaacg
     4441 tgtacgatcc gacggatggc ctcgcctggg gcgctggcta tggtgatgag agcctgatcg
     4501 acggcctgtt ttttggcaat gggacccaat gccgcggtcg tgcccaagac aggaatgccg
     4561 tgaatgacga gtccggtttt cgtggtgtcg tcatcaagaa accctaccgg tttgatacct
     4621 aattccgtgc ggtttgcgat ttcacgagcc accaacgctc ccgcggctcc cgcgcccagc
     4681 agaagcgtac gcacttgttt gtcctttttg gatagcaagg gatgggagaa acgctcggcc
     4741 acaatgcgcc gcaagcctcg aatccccacc gccccagtga aagcaaggaa tgcgtcgatg
     4801 aaaatgactc ccatcggaat gacggcgtat tcggcatacc caaagtgagg cttgatctct
     4861 atcgaaccat gacgaacgaa aaggagaaat ccgttggcaa ccccaaaagc gagtccaaat
     4921 cgaatgacct cgcgcaagct tatgtaccgc caggcaaacc gcgtcacccc ggaaaaaagc
     4981 agcaccagaa attgaaagat gacaatgtaa ggtagcgtaa acagaagctt ttttgtcatc
     5041 aacaacggaa tttgaccatc gaatcgaatc agaaacgcca gcgaatacgc gatggcaagc
     5101 accgccaggt cgatgacgat caaaaacgaa cgatgggcaa accgttgcat catgccgtag
     5161 acccgccgaa tactcgaaac ggacaatgag atgccccagt gacgggatgt gttcatacta
     5221 catggtgcag gtccgttcca gatattcttc cagcgattgc ctgccttccg agcatcgcgc
     5281 gacagcaacg gaagaatctc caatccctct ttcggcctgg tgctaggctc ccgatttacc
     5341 catgacacgt gtctatcttt ctccccccca catgggcggt gaagagctgc gcctcgtcga
     5401 agaagctttc gaaaccaatt ggatcgcccc cctcgggccc catgtcgatg ctttcgagca
     5461 ggagatggcg ctgaaaatcg gtgtgcctca tgcttgcgcc ctgtcgagcg gcaccgcggg
     5521 actgcacttg gccctgctga tgctcggcat agggcgcgac gatgaagtcg ttacctcgac
     5581 actcacgttt tcagcgacag ccaacgccat cacctacgtt ggcgcacaac ccgttttcgt
     5641 cgattgtcat gcatcgacct ggaacatgga tcccgagctt ctggcggaat atctcgagag
     5701 gcgagcagga cagggcaagt tacccaaagc cgtggcggtg gtggatttat atggtcaatg
     5761 cgcggattac ggtccgttgc aggcaacgtg cgcgcgttat gccatcccgc ttttggagga
     5821 tgccgccgag tcccttggag ccacctacca aggacgttcc gcgggtagct tcggtgagat
     5881 ggccgtcttt tcattcaacg gcaacaagat gatcaccacc gccggcggag ggatgctcgt
     5941 atcccacaac aaaacctata ccgatcgcgc tcgatttctg gcaacccagg cccgcgaccc
     6001 cgccccgcat taccaacact ccaccatcgg ctacaactat cgactcagca acgtgcttgc
     6061 cgcgatcggc cggggtcaac ttcgcgtgct ggatgaacgc gtcgcggccc gtcgcgccaa
     6121 cttttccttc tatcaatccg ccctcggaga tcttcccgga ctgacgttca tgccagaagc
     6181 cccttatggc atgagcaccc gttggctcac gtgtatcctc atcgatcccg ccgcgttcgg
     6241 cgcgagccgc gaagacatcc gcctggccct agaaaaacaa gatatcgagt cgcgtcccgt
     6301 gtggaaaccc atgcatctgc aacccgtttt tcaaggctgc gcgaccgtcg gcggtgatgt
     6361 cgccgcctcg ttgttcgaat ccggcctttg tctgacctcc ggctccagtc tatccctatc
     6421 cgataaacaa cgtgtcgtct ccatcattca cgaagttcac gatgcggccc gtcgcggatg
     6481 acgtcgctta caccacccaa cgtcagtcgt cggtggccgc cgtgatgttg aacaacaccc
     6541 gggaagcata acgagaggaa cctccggggg tggaacgcat catcgcaaaa cgctcggcct
     6601 gcacgacctt ttgcagcttg acctgaggtg gcatgccgtt gaggcgaatg ccgttgatga
     6661 aaaaggtggg tgttgcacga accatgagct gccccgccag gttgacgtcg cgctccacca
     6721 cggaacgata ggttcccttg cgaagagtgt cggctaaaga ttgcttgtcc gctcctgctt
     6781 gttccgccca ttgcagcaat tcgtcttgct cccgctcgtc gccatgccgc gtgagggtcc
     6841 cgaacacatc ccaaaaaacc tttcgtcccg acagagccca cgaaccgaca gcgatctgcg
     6901 ccgccgtctc ggacgcggca ccgtccacgg gcaaatgcct tatgctcaaa cgcacatccg
     6961 aaggatattc ggcgagcaat tggctcatgg ctttttgcgc gcttccgctg tgagggcacc
     7021 gcatgtccac gaacaacacc acggtgacgg gcgcatcacg ggtcccgaat acgaccgtgt
     7081 cggcaagtac gggaacgagc gccgaagcgt cttcgggata aaccgcgagg gcttccgacg
     7141 acggtgggcc cgcgggtagc ttgggtacac tcgcatcctc ttgcgaatcc tgagccaaag
     7201 gctgtggtgc atccggtaaa acaagcggtg gcgtgggggc tgcggttggt gccgtcaaga
     7261 ccggtggcga tgaaggaggc ggtggcgtag gggcggcggt tggcaccgtc aagaccggtg
     7321 gcgatgaaga aggcgatgtc gtgctctcag cgaccttcgg tgatgcgtta gacatgtagt
     7381 aaaacacgga cccgactccg cctgccaaca gcagtgcgcc aaggcccacc aacgcatata
     7441 caaaccaagc atccgcgcgt ttcggcgcgg gtgcgtcgaa acggaacaac ggagcgggcg
     7501 tcggcgcggt tacgcgcgtt tgttcttgcg tgctggtgac ccttcgttgt ccgaataggg
     7561 atagttccgg tgttggggcc acttcggcag gctcgggctt ggacggttgt gtgaaaggcg
     7621 ctgggattgg gtcgtcggca ggtgctggcg ctggacgatt ggcctcgaaa cgagcttctg
     7681 acagttttcg cagcagggtt tgcggatcga ggcgacgttg ctgcgcctgc ggatgaagtg
     7741 cttcggacaa tacggcttcg atggaaggag ccagcccatg tcccttggct tcgggcaaaa
     7801 ccgtttggtc ttgcgatccc actcgttttc ccagcaatag ttccaaaacg atccacgcca
     7861 aggcgtacgt atccgtttcc ggagtgggtg cggtggggtg cgggagggat agctcgggag
     7921 gacgtctcga cacggaaagc gcatggcacg ctccgggagg accctgtaac cccatcgcga
     7981 ggtccgacca tccgaatgcg gatagatcgg aacccaggac atcgaaacgc aacgcgctcg
     8041 gttgtaccgc gccatgaacc attccttggt catgaagggc ttgaagagca cgcgcgatcg
     8101 gttcgagcaa tcccagaacg gaatcaatgg atgctggttt tccctcttgc aaccgtgact
     8161 ccaagtttgg gcatggatcg ccagcgtgaa ccatcgcaag acgaggaaca ttgtccacga
     8221 tgacccttcc cgccgcacga ggagccaaaa gagcctggat accgaccgca tgacgagcca
     8281 gccgcgtcaa ttccgtgggt aacgactcca gttgcgcgtc actcacttca atgagcaggc
     8341 tgatgcgaag ggtacctatc gagccgtcgg gggcgagcgc acgaaaggtg gccaccgcat
     8401 catcgaccgc caccagcgaa tcgagccgat gcccggacag cacggttgtt cccaggaggt
     8461 ggtccatcgt gatccaatct tcctttgcga attgcctgtc cagcccctcg tttgcttacc
     8521 gaatgacttc tgccgaaggc tttggtttcg ctagctgtaa catattgatt ttacatattt
     8581 taatgactca ctaacggctc ttccaacgcg attcgcaatt ccgcgccgag ctgctttcct
     8641 agcgtgccgg cttgctgctc ggtggcaggc cacggctcac gtatttccat ccagcgaacc
     8701 cgcgatgcat cgggtgtggc gagcatggcg cgaagccaaa ttttcccctc ttcgtcccga
     8761 caggcatacg ccgcgatcgg cacttggcac gagccttctg ccgcgatcat gacgccacgc
     8821 tcggctgcca ccgccgtggc cgtttcggag tcatgaaggg tagacaacag agccatcgta
     8881 gatgcatcgt cgcttcgaca ctcgatgccg agagcgcctt ggcccaccgc tggcaagctt
     8941 agttcgggcg gcaatacctg ggtggcccga tcgtgcaatt gcaagcgtcg aagcccagcc
     9001 catgccaaca cggtggcgtc tacgacccct tcttgtacct tgcgcaatcg tgtatcgaca
     9061 ttgcctcgca gcggttcgaa acgcaaatcc gggcgtgcct tcgccaacat gatgcgtcgt
     9121 cgagcactcg aagttcctac acgagccgcg tgctcgattt ggctcaagtc atgtccgtat
     9181 cgggatacaa acacgtctcg cggatcttcc cgtttgggaa tacaagccag aacgagcccg
     9241 ggggccagct ccccgggaac gtctttgatg gagtggaccg cgaaatgcgc tttgccgtcg
     9301 atcagggctt gttcgatctc tttgacgaac aaccccttac ctccgatttc cgtcaggggc
     9361 cgatcttgaa cccggtcccc cgtggtgatc acatgcagtt cgtcaattga taggtcgtgg
     9421 gcttgtgcga gccgcgatgc gaaggctctc gattgcgcga gggcgagcat ggatcgccgt
     9481 gtcgccatga tgaggcgtgt ggggatcacg acgcttcctc cggatgggca tctcggtatt
     9541 ccaaagaccg ggagatggga gcggtgtcgg aatgggcggt gggaggcaac gagggagaac
     9601 gagaagaggc tggtatttca tggggatcga tgccgaataa ttcttgaatg gcatcgacca
     9661 gcgttcttgc gttaccctcg gccgccgcgg atttcaaacg tatcgtgggt tgatgggtca
     9721 gttttttgac cgcggccgat agcattccct ccaaggcttt tctttccgcc tctcccaagt
     9781 gcttgagctt gccatgaagg cttttgtcaa gttccgcatc gagcaccgct cggatttggt
     9841 ttcgcaaagc cacgatggtc ggcgtcacct gcaacgagtc ctgccacacg taaaaagcat
     9901 ccgcttcctc cgccacgatt cgctcggata tctgtgcttc tttgaatctg tctcgcaatg
     9961 tcgtggctgc catcttcgat aggtcatcga tatcataaag atacacgcca tcgatctggt
    10021 tgcttcgcgg atccacattg cgcggaacgg caatatcgat gacgaacagg cttcgtcctt
    10081 tgcgctttcg ctgaatcgga gcaagccatt gtggcgtgag caaatagtcc tgactgcctg
    10141 tgcttgcaat gaccacgtcg gtttccaata gcgcgtcctg aagttgattc cactggcgag
    10201 cagagccgtg gaagtgctcg gcgacgatct gggctcgttc gagacttcga ttgacgacga
    10261 ccagacgtgc gcccgcatca caaagatgtc gggcagcagc ttcccccatc gttcccgcgc
    10321 cgatcagcgt gacgacgcgt cggttcaatc caccgaagat ttgcttggca agttcgacgg
    10381 cgacactgga tacggaaacg gctccggctc cgacttgggt ttcggaacgc acacgttttg
    10441 cgacgtggaa agcccattcg atggctcttc cgagcagcgg gccaagcgtg ccttgggctg
    10501 tggcaagatc gaaggcgtct ttgacttgac caaggatctg ggcctcgccc accaccagag
    10561 aatcgagcga tgaactgacg cggaacagat gccggacagc cgcatcgccg atgtagtgga
    10621 ataaatattg gtgcaacacg tcgccaagct ttggtcccgc ttgctcggcc aaagcctgct
    10681 cgatggtggc gatggtttgc tgggccgcat cgtcttcatg acgtccggtt gccgcgtaaa
    10741 tctcgacgcg attgcaggtg gagacgatgc agatttcggc gatttcgggt agctcgtgca
    10801 gactggacag cagagttggc agccggtcgg cggaaatggc gatccgttct cgcacgccaa
    10861 tgggcgcggt tttatgggag gctcccacga cgacaatcat gacccctccc gaaacagata
    10921 gagggtaagc acgatcatcg cgaaaacaaa tccgacgagg gttccatacg cggcgcgacg
    10981 acctcgccaa ccaagcgtgg cccgcagcaa aaggactgct gaaaatacgg cccaagatgc
    11041 ataactgatg agcagacgca cgatctccgt ggttgtgctc agcgagagtt tagcaatcca
    11101 aatcgtaccc gtggccgctc cgaaggaagc cagcaaaaaa cccacgatga caaaaatatg
    11161 agaggctcga tcgagcgaat cgatgggcgg cagccttccg aacacggaag ccccccgctt
    11221 ggttttgagt tgccgctctt ggaccaagta catggtggcg gaccccgccg cgagcacgaa
    11281 aaatgaatcg ccaagaatgt tggcggcgat atggaccggc aacattcccg tgtgcaacgc
    11341 gggaaccacc gcgggttcgg ccatgaaccg gccagccaac aagaaaacga cagcgacagg
    11401 cgcgacgaaa accccgagac cgtagattcg cagcactcga cgcgcgatga gatagacgaa
    11461 agcggcgacg agccctccca tcgacacgcc gaaatgaatc gttttcatgg ggcaaacagg
    11521 ctgggcaatg ccggcaagcc ccaacgcaag agcgtgtaac gaaacgctac cggccaaaat
    11581 ccaccgcgct acgcgcaagg ctttttccca tccaccctga cgcgccaggt gaatggcaaa
    11641 ggcaatggtg gcagcgacat atccaagagc cgccaatccc agcacgatat cgggcaggag
    11701 cgcgtgattc acgggagact acatattctt agcgaggaac tgagccaaca ggtcctcatc
    11761 ggacagcaac gtggaacgag cttctgactc cttttcgtcc tcggctccga ttggggtgcc
    11821 caaaaaacca gggacgatat cgtacgcgtt gttatcgacg atcatggatc gttcaaggat
    11881 gtcggctccc aactcatgca aaaaagcaag cgttttgcat ttgcccacga tgtcgaaggc
    11941 atcggtctcc ccagtgacct ccgtcaaaat acgggcgcct tcggtcagcg cataacgccg
    12001 cccctcgtct ttgagcagca gattttgtcc ttgcaggtcg atggccccgg aaacaagcca
    12061 ggcatcgagg gcttcctgcg gaaaaaagac acggtttttg cccatcatca tcgccatcgt
    12121 agcacgacag ggggtgcagc ttggtcctgg ttgcgctttc gagcaagtcg ttgttggtgc
    12181 ctttttcgag acaagcgcaa gtctattcgg aacggattga ttcgaatcgg aaaggtcgac
    12241 ggtggacacg ggcttccaat ccaggcatct tggcgctgtt catgaccctg aagctggctt
    12301 tggccgccct atgcgtgata gcgaacgcgt ttttcgtcgc cgccgagttc gccctcgtga
    12361 aggtccgtgt gacccaactg gaaacgctcg caaaaaaagg ggaaaaacgc gccgcggtgg
    12421 cgatgaaagt aaccggaaaa ctcgacgctt atctttccgc cacccaactc ggaattacct
    12481 tggcaagcct gggcctcgga tggctgggtg aaccgaccgt ggccgtgctg attgctccgg
    12541 tactccgttt tttcggtatt gagtccgaac ccctcgtgtc cgccgtctcc ctaaccaccg
    12601 cgttttccac cattacgatt ctccatatcg tggtgggaga actggcgccc aaatccctgg
    12661 ccatccagca agccgagtgg gtcgcgctgc gggtggccac gcccatgcgg gcttttttct
    12721 accttaccta tccgtttctg ttcattctca atgaattgtc gaatctgacg ctccgactct
    12781 ttcggattac gcccgtgcag cacgccgaag gcgcattgtc cgccgaggaa atacgtctcg
    12841 tggtcgcagc cggccagctc gaccccaaaa aacgagaact catggaacgc gcgctgcaag
    12901 gaaccgaccg acccgtgcgc tccatcatgg tgcctcgcgt cgatatggaa tacatctccc
    12961 tgctcgattc ccccgagcaa atcatcgctt cgctacgagt caacggctat tcccgtgttc
    13021 ccctcatcga agccaacgat ccggataaaa tcgtcggcta cgtatacctc aaagatattt
    13081 tcgtaggcga tggcttgcct gccggcggta ttcgcgcctt gcgtcgcgat atccttttcg
    13141 tccctgaaaa ccgaaccgtc ggcgatgtgc tcgaggactt caaacgcacc catatcccga
    13201 tcgcgatcgt ggtggatgaa tatggcggga cgagcgggct ggtcaccatg gaggatattc
    13261 tcgaggaaat cgtgggagag ttgcaggacg aactggatgt ggaagccccc ccgatcgttc
    13321 gccgcgatga tggcaccatc atcgtggaag gcgatgtgcc cattcgcgaa ctcgaggctc
    13381 tgggaatcgt cgtagacccc atcgaaggcc acgaaatcgt ggccggtcat atcatatcga
    13441 ggctgggcag actcgctcgc ccgggagata tcgtgagcct agggtcccat gatgccaccg
    13501 tggaagatgt gaggagtcgt cggatcacac gtgtgcgttt ggcacccaag cccgaggagt
    13561 tgcccaaggt cccctctcag gctcctggtc cggtagacga cgcttcgtga acgttggatt
    13621 cgggcgtttt gacagggaga aatggcgtga ctgataagag agtgtcgacg tgggcccctg
    13681 tagtgtg
//
LOCUS       DAOSOR010000083        13611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4540_length_13611_cov_4.805695, whole genome shotgun sequence.
ACCESSION   DAOSOR010000083 DAOSOR010000000
VERSION     DAOSOR010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 13611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13611
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4540_length_13611_cov_4.805695"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            473..3937
                     /locus_tag="PK710_06540"
     CDS             473..3937
                     /locus_tag="PK710_06540"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT09407.1"
                     /translation="MQTAYSTERTSDAIPAQPAAEPCGLARLDERVISGHRGVLVVDT
                     TDASTAHAVRQHVVRRARRLVGRVVLTADAAYLFGCLSDLALRLGIPKLSTNPQVASR
                     QLGEASQGTVVVIEGNVAEGSWDAQVLRFLANNPCLGLFLVIRFGDRNDVLQDEGERV
                     QIVGESKESARVWWEGVIEALVEAGPPGSLASLERWWRGMGGLATWGNASPSVLSEAA
                     QTLLLALQLAHRSWPLTHIDLLGGRQGLEELRQGGLVRIEQGYCVLERAGRAMAKPHP
                     ETLRGVALAMLEVFMDDAWARLRVALLLLEGQAEEQVQEHLARAMVLAGAASQRRDLW
                     RIWCHRLAQGDADKHREISLWSARRALALDDVDAALELAGEARRGLVDPPFEAWLLMG
                     RAQLARGDMVGARVSLEKARQAAVDTSSEARALASEAEVLFWAGELEQAEAFAQRACS
                     MTERASTRLRARNVLGRILLARAKWQQAEESFAEDEALAASERLPDALARARVNRAVA
                     WLSAGQLDVASRVLEAEHQQAQRHALPRANAIALSNLGVIAHIKGRYAHALELYEQAI
                     EACQELGDRLVLARPAINLAELRLELGLVDEAEQALRFAQCTLRAGVPASIAPLISVL
                     EAEIGLERGHTSEAARHAQEALRNAACSSNGGKIGECHRLMAKIALEEGNTNAARQAL
                     VQSQQHIDGARAQAEHAWLEARLARAAGHDATELARNASSLARACGEPEVCRACAVLE
                     AEIAFAQGDIHAAREYLRLARRAFHAMTEGLTPALLRSVRTRKDVRAMDALALEMDRQ
                     NTAREESERQDAPSTSGFVGSDPKVRRLMEMVRRVAIGSNAPVLIRGESGTGKELIAE
                     AIHQHSDRRHKPLVKVNCAALVETLLLSELFGHEKGSFTGAATRRKGRFELAEGGILF
                     LDEIGDISPRTQVALLRVLQDGTFERVGGGTPLHADVRVVCATHRDLAAMVAEGTFRE
                     DLYYRLCGVVIEVPSLRERKRDIHELSNTFLQQIAREQSESVKSLTEEALEILVQHPW
                     PGNVRELQNVLRAASLFADGSIIDAACLFDQIRTPMQEPLEPVPRSGTYKTLVSTQDP
                     VDLAFDSIRHGDTSLTEMKRRIERECIVRALEETGGNITRAASLLGMKRPRLSQIIKE
                     LGLQKGEG"
     gene            3939..4355
                     /locus_tag="PK710_06545"
     CDS             3939..4355
                     /locus_tag="PK710_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09408.1"
                     /translation="MRIHVLVLVSTLALSLGMTGCLFEVSSMGQERDETQESEPLPSV
                     EDWSDLESSTQVGFARSRNPGGDTVPCVGNLKDFSVAKPDPTPWVGAGPNDKPDPTPW
                     SPDSSESSESDPEPGSGESTWGPSGKPDPTPWKSLR"
     gene            complement(4375..7239)
                     /locus_tag="PK710_06550"
     CDS             complement(4375..7239)
                     /locus_tag="PK710_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT09409.1"
                     /translation="MKDFCLPRIARGSRHLACTAALALSAVAFSPSTMGQGAPRDTGL
                     LTGSASPLQAGLDALEQSRYQQARQHLQRAVTGASAQEAWLALGRLGLWEGKYDDVMS
                     AAKQLERLPGRARWQAAVLKAQALAHQGKLSQAIEILRKIENESEARLGRVLLGEYLL
                     LVGDSSAARAPLMTLVQDYNDDRIDENDPEGLALVGRAAYLLRSAADANDAFNEAEKA
                     GAKSTQLLLWRAELFLEKYDTGHAEEVLTELLKKAPNHPDALVFMARVKLDQAYDFAS
                     AEKLIDKALAVNPRHTGAFFLRAGLALREFDLAGADRAVAQGLQCNPNDLDLLSMAAT
                     IRFLADDEPGFASARQAVLQRNPQYSQMYQIIGDFAEWEHRYPEIVAMMQDATQVNPR
                     DGKAWAVLGLNLIRLGEDQPGVEALRKSWQFDRFNVRVYNTLNLFEKDIPNRYDTVLK
                     GSFRFRFDKKERALLERYIPAMMEEAFASMVRRYGFKPELPVGIELYSDPQHFSVRTS
                     GLPAIGIQGVCFGRVLASTSPQAAPFNWGNVLWHELAHVFAIQLSKSHVPRWFTEGLS
                     EYETFLRHPEWQREEDLPLYLALRANRIPKVASFNRAFTHADNPNDITMAYYAASQIV
                     VYIGEQFGMPKIVEMLRLWGQGLRDPQVVQRALGIDMDELDRRFRAWVKPRLVRYDRQ
                     FVPDLRTPSLEDATAAALAAPNDVRTQVDLVLAQAAEGKEKDAETNLAKLRKAHPKDP
                     LVGYVHGHLLMAHKDLPAAQKAFETLISQGHDGYAIRLRLADLAEAQEDLKTMQAHLQ
                     AARRFDPTQVEVLQALHDVAKKQGRKEDMISALRELSVLDQHEPKVAVQLLHALAEAN
                     LWHEARQVGERAILVSLQDPMVHRLYAEALFHTKHYDQALFELESALMCQPEDKKAAQ
                     IYRLAAKVYKTMGRSEDARKAAKKSLELEPDNEEAKALAK"
     gene            7321..8739
                     /locus_tag="PK710_06555"
     CDS             7321..8739
                     /locus_tag="PK710_06555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT09410.1"
                     /translation="MSNGIMSETDLIVLGAGPGGLAAAMEAAMSGMRVVLINGSHLLG
                     YGLHGTYKSKSLWELAKDLQVIHKLGKDFASYVRVDFERLYEQLSRGVEELSAIVLAQ
                     LSRMGITFVPGFARFIDPHRVQVDGQTFTGRTILIATGTRPRHLPEVAVDGYRILTSD
                     HVVDLRRPIQSIVILGAGVLGCEFASIFAAFGMEVSLIDSQPRVLNSEDEDISAFLDR
                     VFERARVRVFRQRHVRSVTTSGDGVRTMLEDGTELDSEAVLVAVGRVPNADRLGLEDI
                     GVMTDSHGYVKVDRCMRTAVPHIYAVGDVARRPDADDICLVHVAEAEGRHAVRSMQGQ
                     DSHLDYGHVPFIIFTHPMIAGVGDNEKMARAKIPEPRMARVEIANNHRLHAMRSFEGF
                     VKLIVGPEGDDRILGVRAVGPQADTLLGEVSVMIEHGIPYSALLQSFHAHPSLSESLH
                     NAAKILAGKYPDDVGLKHAAPA"
     gene            complement(8773..10344)
                     /locus_tag="PK710_06560"
     CDS             complement(8773..10344)
                     /locus_tag="PK710_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09411.1"
                     /translation="MGASGSSDVARLLFLNPLVAPAFAELWFVASSTLGNRGYPGTIC
                     IVSFRAFCCWMLMGLAGCHSEGRGRRASTIDPAAYPPCDVPSDAYQPDKPLNTPGAPV
                     WIRRFGCQADDRAMGVVIEHDGNVVATGSFSGRVSFGDAPLISGGKQDAFVVRWDAKG
                     KPVWSLRLGGLNSEAQGTNIMLGATGELMLAGRFHGVLDATQPQLLSRGLDPFVLMMS
                     KDGSFLWARRFERAGTSEHARVPVAGDAMGGTLVALSPSNPKADDGVVVRVDPSGDIA
                     GQVPLAEGTVASVAVHANRSVLLAGFRGLPHSQKPKGLPRVTGSAPVGLASQLLFLTK
                     IDDQARRVWWREIGDARVRAFGEPLLMVDPQGYGYLAFSYQGEIDVGTGTMDSSGGFD
                     IVMMKLDPAGRTVWARAFAGPQGNEWVNGLAVDPAGHLAVGGAFEAAVDFGGGPIMSA
                     GPISGFVAIFDSLGRHLWSRVFGAQQSRAVVESVSLGAENVAVAGSFQGWTDFGGGLV
                     PSAGQWDGFVALLRR"
     gene            complement(10299..10691)
                     /locus_tag="PK710_06565"
     CDS             complement(10299..10691)
                     /locus_tag="PK710_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09412.1"
                     /translation="MDSKRLSIGSLPVNFKISLEARVRAAAGIPAYMRRKRRIEDLEE
                     AVHRVLEDTYEQALEEHGGDEQTARNIVREQAQRMDLSLLNDLIDRHNRYYPIEANLP
                     TDIKTGRWMLAGKPWEPLAPLTWQDFCS"
     gene            10718..11830
                     /locus_tag="PK710_06570"
     CDS             10718..11830
                     /locus_tag="PK710_06570"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOT09413.1"
                     /translation="MNEKPMLAYLESQKQQGRPIVLAFPILYPREIVTALGACAWEIW
                     SQPLAQPIGSETNRLQAYVCPMVRSAQQVLSSIETIHPRPEAAILPHTCDSMQGLLSI
                     ALSTPAWDLPFLPFRYPRGSHGPASTRFLREEVLAFIASLEKALHRTLDPDSLQQALN
                     LQEQKEDRLRTLLESLRYWQGSDIELYAALRSLEYLHPHDAIPLLDGLLSRVDRQKAR
                     TGVGIVISGMIPEPVDLFDILEDAGGYVAADDYASIGRRLPSRSTARNADPITSVVQR
                     LQRMPPCPTRCSEVQPRIEYLRNLARASGARGIVFHTVKFCEPELFDVASIRKALGED
                     GLSVLHLETEFEAHATGQMATRLEAFIEMLGQGKAS"
     gene            11827..13188
                     /locus_tag="PK710_06575"
     CDS             11827..13188
                     /locus_tag="PK710_06575"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOT09414.1"
                     /translation="MSEKQPTGRGGQMLRRLQYEGAARVVAPALATGLRAQRRLRSWR
                     RPKKEPSEWFGPPLRATYRLKEVLTRHYLSGRYADGAVPVAWVTSGFPIELLRPFGYH
                     VVYPENHAAMCGVQRKGVKLAQVAEVWGLSQDTCGYARCDIGSFLSGNTPAGRLPKPD
                     ILLACTNICQTVVHWYRTLAAILKVPVFVVDTPFLSGDPDERTETYVAEQLEECMQLC
                     ARIAGKKVDRKALGRVAQRSKEASELWGECLALSQAEPAPWTGFEQFLHLGPIVAMRG
                     LPECNAYYRELRDELRERVKHGIGAIRHERFRLLWDNLPIWYELRSLSTLLAEVGFTI
                     VTTTYSYGWAETAPLFDPRDPIVSAARVYARVFINRSLDFRLEMMKKLVNTYSCDGAI
                     LHSNHSCKPYSVGQIDLADRLAKEASIRTLLLDGDHIDMRVHSKEQAEGRLAAFMESF
                     GGL"
     gene            13185..>13611
                     /locus_tag="PK710_06580"
     CDS             13185..>13611
                     /locus_tag="PK710_06580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOT09415.1"
                     /translation="MTIRIGIDVGSTTTKAVAVDDSGRVLARSIEPGDPRIAQQAQRM
                     LEALLERVGGGGEASIVATGYGRKLVAMAGKKRTEISCHALGAFHVMNRPGVLIDVGG
                     QDTKVIRLNSDGSVADFVMNDKCAAGTGRFLEVILARLGV"
BASE COUNT         2735 a         3712 c         4273 g         2891 t
ORIGIN      
        1 cgactcagtt gggacaagaa cagcttccgc caacccgatg gttggatccg ccgtcacctg
       61 cggtctatcc aatggcgacg ttggaagagc ccccgcacgc gactgcgcag gctgctcgaa
      121 atcggtggtg tggggagtgg ggaccggtaa cggtctccgg caacccgatc ggatccgacc
      181 atttgctcag gcggtggaaa tcgcagcaaa gctttgccat gaacgggaag attccgcact
      241 ttgacaaggt gggtgttggt catacatgcc gggccgcggg gttttatcgc catcccaacg
      301 agccgagccc atgcattggt caacatggta acggttacga acgcaggagg acgttagctt
      361 tctgacgtgt cgtcgagcat ctctatcagt ttgctaacgt gtttcgaacc tcgccgttgg
      421 cacgcaatac gaatgcatgg tgcctgcgcc tttccacccc acggtgatgc ccatgcagac
      481 tgcttactcc acggagcgaa cgtcggacgc catcccggca caacccgctg ctgaaccctg
      541 tggtttggct cgactcgatg aacgcgttat ttcgggacat aggggtgtat tggttgtcga
      601 tacgacagat gcctcgacag cccacgcggt gcggcagcat gtagtcagac gtgcgagacg
      661 gttggtgggg cgagtcgttt tgacggccga tgccgcgtat ctatttggat gcctttcgga
      721 tttggcgctg cgacttggta tcccgaagct gtcgacgaac ccacaggtag catcgcggca
      781 gttgggcgag gcgagtcagg ggacagtggt cgtcatcgaa ggcaatgtgg ccgaggggtc
      841 ttgggacgcg caagttctcc gttttttagc aaataatcct tgtttaggat tgtttctcgt
      901 cattcgtttt ggcgacagaa acgacgtatt acaagacgaa ggcgagcgag tgcagattgt
      961 gggggagagc aaggagtcgg cgcgggtttg gtgggaaggt gtcatcgagg cgcttgtgga
     1021 ggcggggccg ccaggatcgt tggcttctct ggagcgatgg tggcgtggca tgggagggct
     1081 tgccacatgg ggaaatgctt cgccatcggt gttgagcgag gctgcgcaga cgttgttgct
     1141 tgcgcttcag ttggctcatc gctcgtggcc gttgacgcat attgacctat tggggggtag
     1201 gcaggggtta gaggagctta ggcaaggtgg tttggtgcgg atcgagcaag ggtattgtgt
     1261 gttggagcgt gcgggccgag ccatggcgaa gccgcatccg gagacgctgc gtggtgttgc
     1321 tctcgccatg ctcgaggttt tcatggacga tgcctgggcg cggctgcgtg tggcattgct
     1381 tttgttggag gggcaagcgg aagagcaggt gcaggagcat ctggctcggg cgatggttct
     1441 ggccggagca gcatctcagc gacgggatct gtggcggatt tggtgtcata ggttggcgca
     1501 gggggatgcc gataagcatc gggagatatc gttgtggtcg gcgcgtcggg cgctggcgct
     1561 cgatgatgtg gatgcggcgt tggagcttgc tggggaggcg cggcgtggat tggtggatcc
     1621 acccttcgag gcgtggctat tgatgggtag ggcgcagctt gcgcgaggag acatggtggg
     1681 ggcacgggta tcgcttgaaa aggcgaggca agcggcggtg gatacgtctt cggaggcgcg
     1741 ggccctggca tcggaggcag aagtgttgtt ttgggcgggg gaattggagc aagcggaggc
     1801 gtttgcgcag cgtgcttgtt cgatgaccga gcgggcgagc acccgtcttc gcgcgcgcaa
     1861 tgtgctgggg cgtatcttgt tggctcgggc gaagtggcag caggcagagg agagttttgc
     1921 ggaggatgag gcgttggcgg cgagcgagcg attacccgat gcgctggcga gggcacgtgt
     1981 caacagggcg gtggcgtggc tatcggcggg gcagctcgat gtggcgagcc gcgtcttgga
     2041 agccgaacat cagcaggcgc agcgccatgc gcttcctcgt gcgaatgcca tcgccttgtc
     2101 gaatcttggt gtgattgcgc atatcaaggg gcgatatgcc catgctttgg agctttatga
     2161 acaggcgatc gaagcgtgtc aggagcttgg ggatcgcctg gtgttggcgc gacccgcgat
     2221 caacctggcg gagcttcgat tggagctggg gttggtggac gaagcggagc aggcgttgcg
     2281 attcgcccaa tgcacgttgc gtgcgggcgt accggcgagc atcgcaccct tgatttccgt
     2341 gctcgaggcg gagattgggc tggaacgcgg gcatacgtcg gaggccgcgc gtcatgccca
     2401 agaggcgctt cggaatgctg cctgttcttc caacggcgga aaaatcggtg aatgtcatcg
     2461 gctcatggcg aaaattgcgt tggaagaggg taataccaat gccgcgcggc aagcgctcgt
     2521 gcaaagccag caacatatcg atggcgccag ggctcaggcc gaacatgcct ggctcgaagc
     2581 acggctggcg cgagccgcgg gtcatgatgc caccgagctt gcgcgaaacg cttcttctct
     2641 cgccagggct tgtggagagc ccgaggtatg tcgtgcttgt gccgtgcttg aagccgaaat
     2701 cgcgtttgca caaggcgata tccatgcggc gcgagagtat cttcggcttg cgaggcgggc
     2761 atttcatgcg atgacggaag gcctgacgcc agcgcttttg cgtagtgtga gaacacgaaa
     2821 agacgttcgc gccatggacg ccctcgcttt ggaaatggat cggcaaaata cggcgcgtga
     2881 ggagtcggaa cgacaggatg cgccatcgac gagcgggttc gtaggcagcg atcccaaagt
     2941 acggcgtctc atggaaatgg tgcgaagggt agcgatcggt tccaacgctc cggtattgat
     3001 tcgcggtgag agtgggaccg gaaaagaact cattgcggag gcaattcatc agcatagcga
     3061 tcgtcgccac aagcccctgg tgaaagtcaa ttgcgcggct ctggtcgaaa ccttgttgct
     3121 atccgagcta tttgggcacg aaaaaggctc gtttaccggc gcggcgactc gtcggaaagg
     3181 aagattcgaa ctggccgaag gtggaatcct atttttagat gaaatcgggg atatttcccc
     3241 tcgtacccag gtcgcccttc tgcgagttct tcaagacggc acgttcgagc gggtgggagg
     3301 tggcactccc ttgcatgccg acgtacgcgt ggtgtgcgcc acccatcggg acctggcagc
     3361 catggtggcg gagggaacgt ttcgcgaaga cctttattac cggctttgtg gagtcgtgat
     3421 cgaggtgcca tcgttgcgcg agcgaaaacg agacattcat gaactatcga atacgttttt
     3481 gcagcaaatc gcacgggaac aaagcgaatc ggtgaagtcg ttgaccgaag aagccctcga
     3541 aatcctcgtc cagcacccat ggccgggcaa cgttcgagag cttcaaaacg tattgcgagc
     3601 ggcttcgcta tttgccgacg gttcgatcat cgatgcggca tgcttattcg atcagatccg
     3661 cactccgatg caagagcccc tcgagccggt tccgcgatcg ggcacctaca aaacgcttgt
     3721 cagcacacaa gatccggtgg atctggcgtt cgatagcatc cggcatgggg acacgagtct
     3781 caccgaaatg aagcgaagaa tcgagcggga atgcatcgtg cgggccctgg aggaaaccgg
     3841 gggaaatatc acgagagccg cgtcgttgtt ggggatgaaa cgtccgcgtc tttctcaaat
     3901 catcaaggaa ctcggtcttc agaagggcga gggttgatat gcgaatacac gtccttgtac
     3961 tggtttcaac cttggcgctc agcctgggaa tgacgggttg cttgttcgaa gtatcgtcga
     4021 tggggcaaga acgagacgaa acacaggaga gcgagccgct tccctctgtc gaggattgga
     4081 gtgacttgga gtcaagcacc caagttggat tcgcgcgcag ccgcaatcca gggggcgaca
     4141 ccgttccctg cgtgggaaat ttgaaggatt tttccgtggc aaaacccgat cccacaccat
     4201 gggtaggggc tggaccgaat gacaaaccgg atcccacacc gtggagccca gattcgtcag
     4261 aatcatccga atcggacccg gagccaggct ccggggaatc gacgtggggt ccttcgggca
     4321 aacccgatcc cacgccgtgg aaaagcctgc gttgatcgca tcatcgcgaa tagactactt
     4381 ggcgagggct ttggcctcct cgttgtccgg ttcgagttca agcgattttt tcgcggcttt
     4441 tctcgcatcc tcggaacgtc ccatggtctt gtataccttg gcagccaatc gatagatctg
     4501 agccgctttt ttgtcctcgg gctggcacat caaggcggac tccaactcga agagcgcttg
     4561 atcgtagtgc ttggtgtgga aaagcgcttc cgcgtacaga cgatgaacca ttggatcttg
     4621 caaactcacc aggatggctc gctcaccgac ctgacgtgcc tcatgccaga ggttcgcttc
     4681 ggcgagggcg tggaggagtt ggacggccac tttgggctca tgttgatcga gaacggatag
     4741 ttctcgcagc gccgagatca tgtcttcttt gcgcccttgt tttttggcta catcgtggag
     4801 cgcttgcagg acttcgacct gggtgggatc gaatcgacga gctgcttgca ggtgagcctg
     4861 catggttttc aggtcttctt gcgcttccgc caggtcggcg agtcgaagcc ggatggcgta
     4921 accgtcgtgt ccttgggaga tcagggtttc gaaggctttt tgagcggctg gcaggtcttt
     4981 gtgggccatg agcaagtgac cgtggacgta gccgacgagg gggtctttgg gatgtgcctt
     5041 gcggagtttg gctaggttcg tttcagcatc tttttctttg ccttccgcgg cttgggccag
     5101 gacgagatcc acttgtgtac gaacgtcgtt gggggcggca agcgccgcgg cggtggcatc
     5161 ttcgagggag ggagttcgca agtcgggcac gaattgccgg tcgtagcgaa cgagccttgg
     5221 tttgacccaa gctcggaagc gacggtccaa ctcatccata tcgatgccga gggcgcgctg
     5281 gacgacttgg ggatctcgaa gtccctgccc ccataatcgc agcatttcca cgattttggg
     5341 catgccgaat tgctcgccga tatacacgac gatctggctg gcggcgtagt aggccatggt
     5401 gatgtcgttg gggttatcgg cgtgggtgaa cgctcgattg aagctggcta ctttggggat
     5461 tcgattggcc cgcagcgcca gatacaaggg caggtcctct tcgcgctgcc attcggggtg
     5521 tcgcaggaac gtttcgtatt cgctcaggcc ttccgtaaac catcgaggta catgggattt
     5581 gctgagttgg atggcgaaaa catgcgcaag ttcatgccac agcacgtttc cccaattgaa
     5641 tggcgcggct tgcgggctgg tggaagccag cacccgtcca aaacacaccc cttgaatgcc
     5701 gatggcggga aggccactgg ttcgcacgct aaagtgttgt gggtcggaat atagctcgat
     5761 ccccacggga agttcgggtt tgaacccata gcgtcgcacc atggaagcaa acgcttcttc
     5821 catcatggcc gggatgtagc gttcgagcag cgcgcgctct tttttgtcga aacggaagcg
     5881 gaagcttccc ttgagcaccg tatcgtaacg gttggggatg tctttttcga ataggttgag
     5941 ggtgttgtag acgcggacgt tgaatcggtc gaactgccat gattttcgca gcgcctcgac
     6001 acctggttgg tcttctccga gacggatgag attgagaccg agcacggccc aggccttccc
     6061 atcacggggg ttgacctggg tggcgtcttg catcatcgcg acgatttcgg gataccgatg
     6121 ctcccactcg gcgaaatcgc cgatgatctg atacatctgc gaatactggg ggtttctctg
     6181 caaaaccgct tgccgggcgg aagcgaatcc aggctcgtca tcggcgagaa agcgaatggt
     6241 cgcggccatg gaaagcaggt cgaggtcgtt gggattgcat tgcaaccctt gggcaacggc
     6301 acggtccgca cccgcgagat caaattctcg aagcgctagc cccgctcgaa gaaaaaaggc
     6361 tcccgtatgt cgaggattga ccgccaacgc cttgtcgatc agcttttccg ccgatgcaaa
     6421 gtcatacgcc tggtcaagct tgacgcgcgc catgaacacc agcgcatcgg gatgattcgg
     6481 cgcttttttc aatagctccg tcaggacttc ttccgcatgt cccgtatcgt atttttcgag
     6541 aaagagttcc gcgcgccaca gcagcaattg cgtagacttc gcgccagctt tctccgcctc
     6601 attgaaggca tcgttcgcgt ccgcggcgct gcgcaataga tacgccgcgc gcccgaccag
     6661 cgctaaccct tccggatcgt tttcgtcaat acgatcgtcg ttgtagtcct gcaccaaagt
     6721 catcagtgga gcacgagcgg cagagctatc tcccacgagc aacaggtact cacccagcag
     6781 cacgcgtccc aaacgagcct cggattcgtt ctctatttta cgaagaattt caatagcttg
     6841 cgataatttc ccttgatgag cgagggcctg cgccttgagc acggcggctt gccaccgcgc
     6901 gcgtcctggc agccgttcaa gctgcttcgc cgcggacatc acgtcgtcat atttgccttc
     6961 ccacagcccg agacgtccaa gcgcaagcca ggcctcttgc gcgctggctc cagtcaccgc
     7021 tcgttgcagg tgctgtcttg cttgttggta ccggctttgc tccaaggcat cgagacctgc
     7081 ttgcagtgga gatgcggatc ccgttagcaa tcctgtatcc cgcggcgctc cttgtcccat
     7141 cgtcgatggc gaaaaagcga cggcagacaa ggccaaagct gcggtacaag ccagatgccg
     7201 tgacccacgg gcgattcgag gcagacagaa atctttcatg ggaacgatga gcgtagcata
     7261 gccgatgccc accccgcgtg tcgggggtta tgcttgggct ggttcggcgt taggaactgg
     7321 atgagcaacg gaatcatgtc ggaaacggat ctcatcgtgt tgggggcggg cccgggtgga
     7381 cttgccgccg ccatggaagc ggccatgagc gggatgcgtg tcgtgctgat caacggctcg
     7441 cacctgcttg gctacggatt acacggcacc tacaagtcca aaagcctgtg ggagctggcg
     7501 aaggacctgc aagtcatcca caagctcgga aaagactttg cctcctacgt ccgcgtcgac
     7561 ttcgaacggt tgtatgaaca gctttcgcga ggcgtggaag aactgagcgc catcgtcctc
     7621 gcgcaactgt cccgtatggg catcactttc gtccctggtt ttgcgcgttt catcgatccc
     7681 catcgggtcc aggtcgatgg ccaaaccttc acagggcgga ccatcttgat cgcgacggga
     7741 acgcggcccc ggcatcttcc cgaggttgcc gtcgatgggt atcgaatcct cacgagcgat
     7801 catgtcgtcg acctgcgtcg tcccatccaa tcgatcgtca ttctcggggc tggggtcctg
     7861 ggctgtgaat ttgcttcgat ttttgccgcg ttcggaatgg aagtgagtct gatcgattcg
     7921 cagccgcggg tgctcaattc ggaagacgag gacatatccg cttttctcga tcgggttttc
     7981 gagcgagccc gggtgcgcgt gtttcgccag cggcacgttc gctcggtcac gacgagcggt
     8041 gacggcgttc gaacgatgct cgaagatggc acggagttgg atagtgaggc cgtgcttgtc
     8101 gcggttgggc gtgttcccaa cgcggatcga ttggggctcg aagacatcgg tgtcatgacg
     8161 gattcacacg gatacgtgaa agtcgatcga tgcatgcgaa cggctgtacc ccatatttat
     8221 gcggtgggag acgtggcgcg tcgtccggat gccgatgata tttgtctggt gcatgtggcg
     8281 gaagcagagg gaaggcacgc ggtgcgatcg atgcaaggac aagattcgca tctcgactat
     8341 gggcatgtgc ctttcatcat cttcacccat ccgatgatcg cgggggtggg cgacaatgaa
     8401 aaaatggcgc gggcgaaaat cccggagcct cgaatggctc gcgtcgaaat cgcgaacaat
     8461 catcgtctgc acgccatgcg atcgttcgag ggatttgtga aattgatcgt cggacccgag
     8521 ggcgatgacc gaatcctcgg cgtgcgggcc gtgggtcccc aggccgacac gctcctggga
     8581 gaggtgtccg tgatgatcga gcatgggatt ccgtactcgg cattacttca atcatttcac
     8641 gcacatccgt ccctgtcgga gagcttgcac aacgcggcga aaatcctcgc gggcaagtat
     8701 cccgatgatg ttgggttgaa acacgccgca cctgcctgaa gctgccgtcg aaggatcgga
     8761 aagcaccctt agtcatcgtc gaagcagggc gacgaatcca tcccattgac cagcggaagg
     8821 gacgagtcca ccaccgaagt ccgtccaccc ttggaatgag ccggcgaccg caacgttctc
     8881 ggctcccagg gatacggatt cgacgacggc gcggctttgc tgcgctccga atacccgact
     8941 ccagagatga cgcccgagag aatcgaaaat cgccacgaat ccactgatgg gtcccgcgct
     9001 catgatcgga cctccgccga aatccacggc ggcttcgaac gcgcctccca ccgccagatg
     9061 ccccgcgggg tcgaccgcaa ggccattgac ccattcattg ccctgggggc cggcaaacgc
     9121 gcgagcccat acggttcgtc cggccggatc gagtttcatc atgacgatat cgaatccgcc
     9181 gctggagtcc attgtccccg tgcccacatc aatctctcct tggtaagaaa aggcgagata
     9241 gccgtatccc tgcgggtcga ccatgagcaa cggttctccg aacgccctga cacgcgcgtc
     9301 gccgatctcc cgccaccaaa cgcgtcgtgc ttgatcatcg attttcgtga gaaacagaag
     9361 ttgcgaggcg agtccgacgg gggcagaacc tgtaaccctt ggtaatcctt tcggtttttg
     9421 cgaatgcggg aggcctcgga agcccgcgag aagcacggaa cgattcgcat gaacggcaac
     9481 gctagccacc gttccctcgg caagaggaac ttgcccggca atatcgcccg atggatcgac
     9541 ccgcaccacc acgccgtcgt cggctttcgg gttggaagga gatagcgcca cgagcgttcc
     9601 acccatcgca tcgccagcga caggcacgcg cgcgtgttcg ctcgttcccg cccgctcgaa
     9661 gcgtcgagcc caaagaaacg aaccgtcctt gctcatcatg agcacgaaag gatcgaggcc
     9721 acgactcaac aattgtggtt gcgtcgcatc caggacaccg tgaaatcgtc ccgcgagcat
     9781 caattccccg gtggccccca gcatgatatt cgtgccctgc gcctcggagt tcaatcctcc
     9841 caagcgcaag ctccacacgg gttttccctt cgcgtcccag cgcacgacaa acgcatcctg
     9901 cttgcctccg ctgataagag gcgcatcacc gaaggataca cgcccggaaa acgaacccgt
     9961 ggcaaccacg ttgccgtcgt gctcaatcac cacgcccatg gcccgatcgt ccgcctggca
    10021 tccaaagcga cgaatccaca cgggcgcacc aggagtattc aggggtttgt ccggttgata
    10081 ggcatcggat ggcacatcac aaggcgggta ggccgcaggg tcgatggtgc tggctcttcg
    10141 accgcgccct tccgaatgac aaccggccag gcccatgagc atccaacagc aaaacgcacg
    10201 aaatgacacg atacagatgg tacccggata cccgcgattg ccaagcgtgg aggatgctac
    10261 gaaccacagt tccgcaaaag caggggccac aagcggattc aggaacagaa gtcttgccac
    10321 gtcagaggag ccagaggctc ccatggtttt ccagcaagca tccaacgccc cgttttgatg
    10381 tccgtcggca agttagcctc gatcggataa tagcggttgt gacgatcgat cagatcattg
    10441 agcaaactca agtccatgcg ctgggcttgt tcccgcacga tgtttcgcgc ggtttgctcg
    10501 tctcccccat gctcctcgag ggcttgctcg taggtatcct ccaggactcg atggacggct
    10561 tcctccaggt cctcgatgcg tcgttttctg cgcatatacg cggggatacc ggcagcggcg
    10621 cgcacgcgcg cctccaggga gatcttgaag ttgacgggca aagacccaat gctcagcctc
    10681 ttgctgtcca tcggtgtaca acgttatcac gcctttcgtg aacgaaaaac ccatgctggc
    10741 gtatctcgaa tcgcaaaaac aacaaggtcg ccctatcgtg ctcgcctttc ccatccttta
    10801 tccgcgggaa attgtcaccg cgttgggagc ttgcgcgtgg gagatttggt ctcaacctct
    10861 ggcgcaaccc atcggcagcg aaaccaacag gcttcaagcc tatgtgtgtc cgatggtgcg
    10921 aagcgcgcaa caggtgcttt cttcgataga aaccattcat ccacgaccag aggcggccat
    10981 cttgccccat acctgcgatt cgatgcaggg gttgctatcc atcgcgctct ccactcccgc
    11041 ctgggatctt cctttccttc cctttcgata tccacgaggt tcccatggcc cggcgtcgac
    11101 gcggtttttg cgtgaagagg ttcttgcgtt catcgcctct ctcgaaaaag cgctgcatcg
    11161 aacgctcgat cccgattcgt tgcagcaagc gttgaacctg caggagcaga aagaagatcg
    11221 gctgcgaacc ctgctcgagt ctttgcggta ctggcaaggc tcggatatcg aattgtacgc
    11281 ggcattgcgt tccctcgaat atttgcatcc ccacgatgcc atccccctgc tcgatggtct
    11341 gctatcccgg gtggaccgac aaaaagcgag gacgggcgtc gggatcgtca tcagcggcat
    11401 gattccagaa cctgttgatt tatttgatat tttagaagat gcgggcggat atgtcgccgc
    11461 ggatgactac gcctcgatcg gacgaaggct tccttcgcgc tcgacggctc gcaatgccga
    11521 ccccatcact tccgtcgtgc agcgcttgca acggatgccg ccttgcccca cacgttgttc
    11581 tgaggtgcaa cctcgcatcg agtatcttcg caaccttgca cgcgccagcg gcgcacgggg
    11641 gattgtattt cacacggtga agttctgtga gccggagttg ttcgacgtgg catcgattcg
    11701 aaaagcgttg ggggaggatg ggttgagtgt gctgcatctg gagacggaat tcgaagcgca
    11761 tgcgaccggt cagatggcca cacgattgga ggcattcatc gaaatgcttg gccaagggaa
    11821 agcgtcatga gcgaaaagca gcctaccggg cgaggcggcc agatgcttcg ccgtttgcag
    11881 tatgagggcg ctgcacgcgt ggtagcgccg gcccttgcga ccggcctgcg cgcgcaacgt
    11941 cggctgcgtt catggcgaag accgaaaaaa gagccgagcg aatggttcgg gccgccgctt
    12001 cgtgcgacct atcggttgaa ggaagtgttg acacgccatt atctatcggg gcgatacgcg
    12061 gacggggcgg ttcccgtggc gtgggtaact agtggttttc ccatcgaatt attgcgaccc
    12121 ttcggatatc acgtcgtcta tccggaaaac cacgccgcga tgtgcggggt gcaacgaaaa
    12181 ggcgtgaagc tcgcgcaagt ggccgaagtc tgggggcttt cccaagacac ctgcggttac
    12241 gcgcgatgcg atatcggctc ctttttgtcg gggaacacgc ccgccggacg cctgcccaaa
    12301 ccagatattt tgctggcctg caccaacatc tgtcaaaccg tcgtgcattg gtatcgaacg
    12361 ctcgcagcga tcctgaaggt tccggtattc gtggtcgata cgccattttt gtccggcgat
    12421 ccggatgagc gcacggaaac gtatgtggcc gaacagctcg aagaatgcat gcaattatgc
    12481 gcgcgaatcg cgggcaagaa ggtcgatagg aaagcgttgg ggcgcgtggc ccagcggtcg
    12541 aaggaggcct ccgagttgtg gggcgaatgc cttgccttat cccaggccga acccgcgccg
    12601 tggacgggat tcgagcagtt tttgcacctc ggcccgatcg tggcgatgcg cggactgccc
    12661 gaatgcaacg cgtattatcg agaactgcgc gatgaactgc gagagcgtgt caagcacggc
    12721 atcggcgcca tacgccatga acgcttccgc ttgctatggg acaacttgcc catctggtac
    12781 gagcttcgtt ctctatcgac gcttcttgcg gaggttggct tcaccatcgt cacgaccacc
    12841 tattcgtacg gctgggcgga gaccgcgccg ctatttgacc cgcgggatcc catcgtcagc
    12901 gcggcccgcg tgtatgcgcg agtattcatc aatcgaagtc tcgattttcg cctcgagatg
    12961 atgaaaaaac ttgtaaatac atatagttgc gatggggcga tcctgcacag caaccattcg
    13021 tgcaaaccct attctgttgg ccaaatcgac ctggcggatc gtctcgccaa agaagcttct
    13081 attcggacgc tattgctcga tggggatcat atcgacatgc gcgtgcatag caaagagcag
    13141 gcggaaggga ggctggccgc gttcatggaa tcgttcggag gattatgacg atccgaattg
    13201 gaatcgacgt ggggtcgacg acgaccaagg cggtagcggt ggacgacagc ggtcgtgtgc
    13261 tggctcgttc gatcgaaccg ggggatccgc gcattgccca gcaagcgcag cgcatgctcg
    13321 aggcgttgct cgaacgcgtg ggaggcggtg gcgaagcatc gatcgtggca acgggctatg
    13381 gccgcaagct ggtggcgatg gctgggaaaa aaaggacgga gatttcgtgt catgcgctgg
    13441 gggcatttca cgtcatgaat cggccgggag tgctgatcga cgttggtggc caggacacga
    13501 aagtgatccg attgaattcg gatggttcgg tggcggattt tgtgatgaac gacaagtgtg
    13561 cggcgggaac ggggcgtttt ctcgaggtga ttttggcgcg gcttggggtt t
//
LOCUS       DAOSOR010000084        13591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4551_length_13591_cov_4.418587, whole genome shotgun sequence.
ACCESSION   DAOSOR010000084 DAOSOR010000000
VERSION     DAOSOR010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 13591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13591
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4551_length_13591_cov_4.418587"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PK710_06585"
     CDS             <1..494
                     /locus_tag="PK710_06585"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HOT09416.1"
                     /translation="SSLPNVLRVVLGAPVVEKQSQVSPTHTVIETNVDDMTGELAAHT
                     IRILMDSGARDVWVTPTTTKKGRPGMVLSVLAAKEDVARLEEAILRETTTLGVRKIDV
                     TRTELPRKESEVETRFGAIAVKLAEQGDSVRMKPEFDACVRAAQQHGVTVREVIEEVM
                     KRR"
     gene            593..1375
                     /locus_tag="PK710_06590"
     CDS             593..1375
                     /locus_tag="PK710_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09417.1"
                     /translation="MAENDHAKQARAYLEHLCQRMDRGEAPVRSSKAWWQLPSFIPVA
                     VGLSLVACGGTVQEESKGGDTKEICDNGKDDNGDGKIDCDDSDCAEFPGCLSAAYAAP
                     VEKDCRDGLDNDGDGKVDCDDSDCVGLPECQVVGPAYAAPVEMDCHDGLDNDGDGLTD
                     CLDSDCIMAPGCTAPAYAAPVETDCQDGQDNDGDGEVDCDDSDCADVPACTAGPAYAA
                     PVEADCEDGKDNDGDGDIDCADSDCYNDPACMSAAYAAPFSP"
     gene            complement(1497..3278)
                     /locus_tag="PK710_06595"
     CDS             complement(1497..3278)
                     /locus_tag="PK710_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09418.1"
                     /translation="MTDTAPLQVKSFEVPQANSLDRIRRLVEAVSRGATQKREIQAAT
                     GLSSRHADYYGQAARILGWLEREGETLALAPNAMALLRTPPGSDEERDVFAESIEACE
                     VVQALCPDLLQEQGPSEDELTQRIRSATDLAQKTARRRAQALLAWRDRVVVSPSEPDQ
                     DIVSLLPDESTQGQGLATLHARFDSLSIERFKGIRQASVGFSALTLLAGPHGSGKSSF
                     REAFRFLHGIGRNYSLSQIAGEHWGDTGTLAWPGIPGGLQELARLGHNTFSIEIGVAI
                     PEGNQLRNANYFIEVTVEDAASPRVVRERLSLEDKGAYIFDSHPPQDAPILVPDRLSI
                     RLGRTSRTGAKGPTLLVDPRRPALSQLAMHPAVRSRTVVDATSSVLSVFRSLRFLQPT
                     LQAMQRPSFPHQVVLGSHGDHLSTCLHAICQDPERRDLVFRSLRSFSDGLLHDLQWLP
                     DATGRLVLVILDRNGMKLTAPSISEGVLRFLTMLCAVVGFDNPSFWFVDNADMGFCPS
                     KLSFVLDLLQRQCSGGHRQLVLTVRDENAVETLVSGKEYLSSAYRLFAHGWRSPERMG
                     AREHTPPARPLRHPLRDEDDPNERLPF"
     gene            3481..4542
                     /locus_tag="PK710_06600"
     CDS             3481..4542
                     /locus_tag="PK710_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT09419.1"
                     /translation="MIEVRNLFKYYGQRRAVGPLSFSIAPGEIVGLLGLNGAGKTTVL
                     RVLACDLLPSAGTVTVDGIDVVDQPQQVRHRIGYLPDTPPVYAEMGVHEYLVFAARLR
                     GLTTQEAHRRATEAEKVTNLEKVAHDPIHSLSHGFKQRVGIAQALVHRPKLLLLDEPI
                     LGLDPVQIVEMRHLLRSLRGDFTIVLSSHNLKEISETCDRLLVIREGSIVASGSEAEL
                     SSKLLQTTSVEITFSGDHERVERVVRDKDGVRSVALHPSPEDPGNHVLRVDADRDIRP
                     ELVRTLVAAEVDVLQVVQAKGELESIFLRLAGDVSAAGNDDPACDGSNPESPSQQDRA
                     MNGIEPHDSTDKKVDKEED"
     gene            4542..5267
                     /locus_tag="PK710_06605"
     CDS             4542..5267
                     /locus_tag="PK710_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT09420.1"
                     /translation="MRSALLIASRELRGFLRSPLAYVVIAIMLLIDGLLYYGVALRGE
                     ARLSAEVLRDFFYFSSGTTMTTAILLSMRLFAEEKQTGTFVLLNTAPVPDSAIVAGKY
                     LSAYTLVAGFTLMTVYMPLLIFVHGRVSVGHIVVGYLGLLLIGAASTAIGTFGSVLVK
                     SQTIAAVISAVLLVFMLVLWLVARETDPPIREFLTALSLHDTHFRPFMLGQLELQHCV
                     YYLAVTFFFLLATTKTLEARRWR"
     gene            5264..7165
                     /locus_tag="PK710_06610"
     CDS             5264..7165
                     /locus_tag="PK710_06610"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOT09421.1"
                     /translation="MKRLALSSAITLPVTLVGLVLLFVGERIIQETASARIALSGLGM
                     TLVVAMVAFRIVIAHRAEGQHKTSERTMAIVALVGGFAVVGYFTTAEPFSAWMVGGMS
                     SAAQQRFGAVMTVAWIVLLLISVVPQVFGEIAMWPMRHAEHIEWRRVRDAVYAGLTLA
                     FAASYGALAVYGASELGVRADYSYFKTSRPSESTLAMLAPMAEDVRVIAFFPPVNEVR
                     DEVMGYLRELQRQSPNVRLEEQDRLLAPALAKEAKVFQDGVIVLMRGAQTESIAVGTE
                     IKAARKTLRQLDTEMQKALTKVLRAQRTAYLTAGHGELNDTKLDDVATGRSALGVKEL
                     LEVQNYVIRDLSFVQGLGRDVPEDATVVLVLGPTEPFAPEELASLERYAARGGRLLLA
                     LEPEGKANNEQLAKIAGLKFNEGLLCNEESHGIRRRNPSDHAIVFSNRFSTHPSVASL
                     RRLGSRAVFFLNAGSIDKQDDAAGEIDFSIQSLQGTWADLNGNFLFDDSDETKGVFRL
                     VASVTRPLEDKKNSKENGTENEKQEPVETRMVVVADGDALSDATLMNPSHFNGNPQFV
                     VDVIRWLGGEEAFSGLVASTEDVRIEHTKQKDKAYFYLTIFGVPALVLAMGWTGLRRR
                     RRAKEGRKA"
     gene            7162..8208
                     /locus_tag="PK710_06615"
     CDS             7162..8208
                     /locus_tag="PK710_06615"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOT09422.1"
                     /translation="MNPVRSLWVHGALLGLACLGAVMVWTRDQQSTSMSVADVRVWSA
                     KPAEVQKIVFESKSRSVVLESKNDEVGRWFEGTVTRQQMPRPKQDKQEADATNHDPDT
                     SAVDNKGEENGMLTVFPVLSVTTAERIAEAVAPLRAIREVGHLSPDQRQDFGLAEPEG
                     MLILTIAGVERKLEVGGVAPGGADRYIRNPDTDISYAIRGEFCRDLLSGESMLNEREL
                     HGFEEDEIKRVRIDANGKSREILRAGAGMTASWTDASTPDKPDESLAAWMAKVARLRP
                     TEYLMAPPAQAVPIMRLELFGKKDSLGFLELLRLPDEKAGYVVRTEYTRKHAKVFPSA
                     GEQVEQELDSLLRQ"
     gene            complement(8324..10495)
                     /locus_tag="PK710_06620"
     CDS             complement(8324..10495)
                     /locus_tag="PK710_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09423.1"
                     /translation="MKRFPWFGFCLGLLAISTGCANTPTSKTPSPILPLRTVRLYETG
                     VGYFERTGEVSMQEGTSLPVPAAHVDDALKTLIVVSGKGRTTVDGLEFDSSVTEGMAR
                     ALAALPKTQDTEITYLNLLSSLKGESVVVRTPKSTIEGRIINIVVEENPEKPNSSESP
                     PDSDESNSKTPVTQTLRVLILSANKEILNIVASDITSIRPLSSVTQARLDLALNALST
                     RGSQSRRLIRVMANAQGPVTLGYIAETPLWRTTYRLVLDDTKPEAMLQGWALVHNDTD
                     ENWQQVRVELVNGRPDSFLFPMAAPRYGRRPLVTPETDLSTIPQLLDRTPDGIWGDHA
                     DIDEYQSYGTGYGYGAGYGSLSGSRRASAPSVRMGATMSQSPPEASERLSVGNLASVR
                     QAEGVESAALFIYSLPRPVDLRAHGSALVPFVQSSVTSPRITWVDSQEAPQMGVLFTN
                     RAGQTLPAGPVAVFSDGGFAGESTLDRLKPHEQRYLLFGVDLDVEAKKKTDAEVHAAQ
                     LIVLEKDTLVEHFTTTQDLRFLMENRSGQSRAVHWALSLVDNANVEGADEVRYDKESD
                     KYILVFHVPAHEKAERKVRIIQGFQQKHSLDTITFDRLDSLTTQSTLSASQRNVLDTA
                     KEQQKKLEQTRSLIADMDEAMKRIESDLERFREHLKALGGEKGAGAAANPFVKRILDA
                     EDQLTLLRTKRQTLVDRSHEEQKTIRRTLEPLVAPATSSQP"
     gene            complement(10586..12004)
                     /locus_tag="PK710_06625"
     CDS             complement(10586..12004)
                     /locus_tag="PK710_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09424.1"
                     /translation="MKPSSFRTIAVVLTALGCGSAQSTVSSPPASSSAHEASSNDWQG
                     RSDSAAEESTPSPCYAQCRELIDKANAVFQQAQGEDEPARRRTMDRAGEAYIAAWRGC
                     SLETHVGMDLQCEGASLVAPRMLDSFRASDRYDRAIFAILVTRDRRWRTDTAPTNQEL
                     KTAVSAAENAFASQPQAAQAREWVRMAAYGRIALGDVSFAQKDMESFVKGASKSEQQE
                     HAAMRVAMASRLNDQGEHASAQRLLGTTSPEEPARVAAGWHAEMGRALAGQNRSAQAT
                     FDRALAVWQAVPSSQWRQQLGPAEPWPMWDRERGIETIGAAHFYSAERLRTQTVLPFP
                     AYRGSGAMKDVAEYLETKVGSYVRERQARLLKAEEAYQKISEIQPVAPSRWLVASACR
                     IGQMWAALVQDVTQSPLPPSIAKDPDIAAGYRKTLQESLRPVDEKARRAFIICRDTAQ
                     RFGIDDDFSRACHSWLADNPEP"
     gene            12106..13110
                     /locus_tag="PK710_06630"
     CDS             12106..13110
                     /locus_tag="PK710_06630"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOT09425.1"
                     /translation="MRLLDVSSAVARRAVRSGAPVFIPINPIEYHGPHLSLHNDSWIS
                     RGMMHDLYSRMLSEHPEWEWLEFADLECGVQPVPGPGSRGVPFDVMRRLVLDVCRSVA
                     ELGAKRVVLMTFHGSPLHGIALEHGVKALRRWGVHAVAPFNIMLRKLLDAPGSVFSDA
                     YDVLGSSSERDIMQAAFQSDFHGGFLETSVALHYASSTVDSCFEKLPPCPAVLPDRGL
                     ELAATIARKAGRSDLARELHIGAYGQGWYALRPFPGYTGNPSLATAEAGKAFARTFVE
                     LAWKDVLSALHGRPSALVPLGWWFEYASMGGKLSLPRVGLDDVFVHAPMVSEAAHELT
                     "
     gene            complement(13133..13205)
                     /locus_tag="PK710_06635"
     tRNA            complement(13133..13205)
                     /locus_tag="PK710_06635"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13170..13172),aa:Phe,seq:gaa)
BASE COUNT         2848 a         3604 c         4104 g         3035 t
ORIGIN      
        1 ggtcgtcgct tcccaacgtg ctccgtgtcg ttttaggagc cccggttgtc gaaaagcaga
       61 gtcaggtttc acctacccac acggtgatcg aaacgaacgt ggatgatatg accggtgaac
      121 tcgctgctca taccattcgt atcctgatgg atagtggggc gcgcgatgtg tgggttactc
      181 ccacaaccac caaaaaaggg cgtcccggaa tggtgctgtc cgtgttggcg gccaaagaag
      241 atgtggcgcg gctcgaagag gcgatactcc gtgaaacgac gacgctgggc gttcgcaaga
      301 tcgatgtgac gagaacggag ttgccaagga aagaatccga agtcgagacc aggttcggag
      361 ccattgcggt gaagctggcg gagcaggggg atagtgtgag aatgaagcct gaattcgatg
      421 cttgcgttcg cgccgcccag cagcacggcg tgacggtgcg agaggtgatc gaagaagtaa
      481 tgaaacgtcg ctgatattcc ctaacgtaag aaatcattat tgatattgcg gacattgcgg
      541 cgccatgtca tcgttttcac tcccattgtc ttttttttga gagggatgag tcatggctga
      601 aaacgatcat gcgaagcagg ctcgcgctta tctggagcat ctttgccagc gaatggacag
      661 aggggaagca cctgttcgtt cgagcaaggc gtggtggcag cttccatcct tcattcctgt
      721 agccgttggc ttgtccttgg tcgcgtgcgg cgggacggtg caggaagaga gcaaaggggg
      781 agatacgaaa gagatttgtg acaacggcaa agacgacaat ggggatggga aaatcgactg
      841 cgatgattcg gattgcgccg agttccccgg gtgtctttcg gctgcctatg ccgcgccggt
      901 agaaaaggat tgtcgggatg gtttggataa cgacggcgat gggaaggtgg actgcgatga
      961 ttcggattgc gtgggtctcc ccgagtgtca ggtcgtgggt ccagcctatg cggcgccggt
     1021 ggaaatggat tgccatgatg gtttggataa cgacggcgat gggctgaccg attgcctgga
     1081 ctctgattgc atcatggctc ctggatgcac cgccccagcc tatgcggcac cggtggaaac
     1141 ggattgccag gatgggcagg ataacgacgg cgatggggag gtagactgcg acgattcgga
     1201 ttgtgctgat gtccccgcgt gtaccgcggg ccccgcctat gcggcgccgg tggaagcgga
     1261 ttgcgaggat gggaaagaca acgatggcga tggtgacatc gactgtgcag attcggattg
     1321 ctacaatgat ccggcgtgta tgtcggcggc gtacgcggct ccctttagtc cataatccct
     1381 aaatattacg catagttagc attcctcggc cgaaccgtcc gacctgtcgt tctttccgtg
     1441 ctcatggcgg aatcatcggg actggtgcat cgattcttca gtccacggca tgaggctcag
     1501 aacggtagac gctcattcgg atcgtcttca tcacgtagcg gatggcgcaa aggacgagcg
     1561 ggaggagtat gttcacgagc gcccatccgt tccggagatc gccatccatg cgcgaaaagc
     1621 cggtaggccg atgataggta ttccttgccg gatacgagcg tttccacggc attttcgtcg
     1681 cgtacggtga gcacgagttg tcgatggccg ccactgcact gacgctggag caagtcgagc
     1741 acgaagctga gtttcgatgg gcaaaagccc atatccgcgt tgtcgacgaa ccagaagctt
     1801 gggttatcga aacccaccac ggcacataac atggtcagga aacgaagaac tccttcggaa
     1861 atgctgggag cggtcagttt catgccattg cgatcgagga taacgagaac gagtcttccg
     1921 gtggcgtcag gtagccattg aagatcgtgg agcaatccat ccgaaaagga gcgcaggctg
     1981 cgaaacacca ggtcacggcg ttctggatct tggcagatgg catgcaggca ggtggataga
     2041 tgatcgccgt ggctccccaa cacgacttga tggggaaagg aaggtcgctg catggcttgc
     2101 agggtgggtt ggagaaatcg aagggatcga aaaacggata agacggaaga tgttgcatcc
     2161 acgacggtac gacttcgcac ggctggatgc atggcgagtt gggatagggc agggcgacga
     2221 ggatcgacca acagggttgg ccctttggct ccggtacggg aggtgcgtcc gagacgaatg
     2281 gaaagccggt ccggaacgag gattggcgca tcttggggag ggtgcgaatc gaatatataa
     2341 gctcctttgt cttcgagcga caagcgttca cgaacgactc gcggtgaagc ggcgtcttcc
     2401 accgtaacct cgatgaagta gttggcgttg cgaagctggt ttccttcggg aatggcaacg
     2461 ccgatctcga tggaaaaggt gttgtgtccg aggcgagcca gctcctgcaa accaccgggg
     2521 atgccgggcc atgccaacgt gcctgtgtct ccccaatgct ctcccgcaat ctggctgagc
     2581 gagtagttgc gtccgatgcc atgcaagaac cgaaacgcct cgcggaagct gctctttccc
     2641 gaaccatggg ggccggccag aagggtgaga gccgaaaagc ccacggacgc ttggcgaatg
     2701 cctttgaagc gttcgatcga caacgagtcg aaccgtgcat gcagtgtggc caagccttgt
     2761 ccttgcgtgc tttcatcggg cagcaaggaa acgatatcct ggtcgggttc cgatggactg
     2821 accacgaccc gatcgcgcca ggcgagcagc gcttgagccc ttcgacgcgc ggttttttgc
     2881 gcaagatcgg tggcggagcg aatacgctgc gtcaactcat cttcggaagg tccttgttct
     2941 tgaagcaaat ccgggcagag cgcttgcact acctcacaag cttcgatcga ttccgcaaat
     3001 acatctcgtt cttcgtcgga gccggggggt gttcgaagaa gcgccatggc gtttggcgcg
     3061 agcgccagcg tttctccttc acgctcgagc catccgagga tgcgagcggc ctgtccgtaa
     3121 tagtcggcgt gtcgcgacga caacccagtg gctgcttgga tctcgcgttt ttgtgtagcg
     3181 cctcggctca ccgcttccac cagtcggcga attctgtcca aggagttggc ctggggcacc
     3241 tcgaaggatt tcacctgaag cggggcggta tccgtcatca acgactccca caacgagcaa
     3301 gctcagcgtt ctcctgcata gcctggagcc acggggacct caatcgatac gaacggctca
     3361 ctccaagcat gtcacgaatt tctcaaaccg atgatcaact cttcgatccc gggaatgaat
     3421 ggcgttttcg ttggttctga agctggccgt ccgtccactt ctgttctatc gggaaccgtc
     3481 atgatcgagg ttcgaaacct gttcaagtat tatgggcagc gaagggccgt gggcccgctg
     3541 tccttttcta tcgctccggg agaaatcgtc ggtcttctcg ggctcaatgg tgccgggaaa
     3601 accacggttc tgcgtgtttt ggcctgtgat ctgctccctt ccgcgggaac cgtgaccgtc
     3661 gacggcatcg atgtcgtcga tcagcctcaa caagttcgac atcgaatcgg atatttacct
     3721 gatactcctc cggtctatgc agagatgggg gtgcatgagt atctcgtatt cgccgcgcgt
     3781 ctgcgaggct tgacgacaca agaagcccat cgccgcgcga cggaagccga aaaggttacc
     3841 aacctggaga aggtggctca cgatccgatt cattcgctgt cccacggatt caaacagcgt
     3901 gtgggtatcg cccaagcgct cgttcatcga ccgaaactct tgttgctcga tgaaccgatc
     3961 cttgggctcg atcccgtgca aatcgtagag atgcgacact tactgcgaag ccttcgcggc
     4021 gattttacca tcgtgttgag ttctcataac ctgaaagaaa ttagcgaaac ctgcgatcgc
     4081 ctgctcgtca ttcgagaagg ttccattgtc gcttccggtt ccgaggcgga gctatccagc
     4141 aaactgcttc aaacaacgag cgtcgaaatc acgttctctg gcgatcacga acgcgtggaa
     4201 cgagtggttc gggataagga cggggtgcgg agcgttgccc tacacccttc accggaagat
     4261 ccgggaaatc acgttcttcg ggtcgatgcg gaccgcgaca tccggccaga attggttcgt
     4321 accttggtgg cggctgaggt cgacgtgctg caagtcgttc aggccaaagg agaattggaa
     4381 tccattttct tgcgtctggc gggcgatgtc agcgcggcag ggaacgacga tcccgcttgt
     4441 gatggttcga atcccgagtc cccttcgcaa caagacaggg caatgaacgg catcgaacca
     4501 cacgattcga cagacaagaa ggtggataag gaggaagact gatgcgaagc gctcttctca
     4561 tcgcatcccg agagcttcgg ggatttttgc gctcaccgct cgcgtatgtc gtcatcgcca
     4621 tcatgctgct catcgatggt ttgctttatt acggtgtggc tcttcggggt gaggcccgct
     4681 tgtcggccga agtgctgagg gattttttct atttttcgag cggcaccacc atgacgaccg
     4741 cgattcttct gtccatgcgc ctgttcgccg aggaaaaaca aacaggcacc ttcgtgctgc
     4801 tcaataccgc tcccgtccct gattccgcca tcgtggcagg aaaataccta tccgcctaca
     4861 cgctcgtggc cggcttcacc ctcatgaccg tgtacatgcc gttgctcatt ttcgtgcatg
     4921 gacgtgtttc cgtcgggcat atcgtggttg gctatctggg attgttgctc atcggcgccg
     4981 cgtcgacagc cataggaacg tttgggtccg tgttggttaa aagccaaacc attgcagccg
     5041 tcatttcggc ggttcttctc gtgttcatgc tcgtgttatg gctggtagcc cgcgaaacgg
     5101 accctccgat tcgcgagttt cttaccgccc tatcccttca cgacacccac ttccgtccgt
     5161 tcatgctcgg acaactcgaa ctacagcact gcgtttacta ccttgccgtg acgttctttt
     5221 tccttcttgc caccacgaaa acgctcgaag ccaggaggtg gcgatgaaaa ggttggcttt
     5281 gtcttcggcg atcacgctcc ctgtcacgct tgtgggactc gttctgcttt ttgtgggtga
     5341 acgaatcatt caagaaaccg cttccgcgcg tatcgcccta tctggtctgg gtatgacgct
     5401 cgtcgtggct atggtggcct ttcgcatagt cattgcgcac cgggccgaag ggcaacacaa
     5461 gacctcggaa cggacgatgg ccattgtggc tttggtaggt gggtttgcgg tcgttggata
     5521 cttcactacg gctgaaccgt tttctgcatg gatggtgggt ggcatgtcca gtgctgctca
     5581 gcagcgattc ggtgcggtga tgaccgtggc atggatcgtg ttgctgctga tttcggtggt
     5641 gccccaggtg tttggggaaa tcgcgatgtg gccgatgcga catgcggagc atatcgaatg
     5701 gcgaagggtc cgagatgcgg tttacgcggg gttgacgctt gcgtttgccg caagttatgg
     5761 cgctttggcg gtgtatgggg ccagcgaatt gggggtgcgc gcggattatt cgtacttcaa
     5821 aacctcacgg cccagcgaat caaccctcgc catgctcgcg ccgatggccg aggacgtgcg
     5881 tgtgatcgcc ttctttccac ctgtcaatga ggttcgtgat gaggtgatgg ggtatttgcg
     5941 ggagctgcaa cgacaatcgc cgaatgtgag actcgaggag caagaccgac tccttgcacc
     6001 ggctctcgcc aaagaggcca aggtgtttca ggatggtgtg atcgtgctca tgcggggagc
     6061 acagacggag agcattgctg ttggtacgga gatcaaagcc gcgcgcaaaa cgctgcgcca
     6121 actcgatacc gagatgcaaa aagcactgac gaaagtgctt cgcgcccaac gcaccgcgta
     6181 tttgaccgcg ggtcacggtg aactcaacga tacgaaactc gatgacgtgg cgacggggcg
     6241 aagcgcgttg ggagtcaagg aattgctcga ggtgcagaat tacgtgattc gagacctgag
     6301 cttcgtacag ggattggggc gcgatgtgcc cgaggatgcc acggtggtgt tggtgcttgg
     6361 tcccaccgaa ccgttcgccc ccgaagagct ggcctcgttg gaacgctacg ccgcacgagg
     6421 cggaaggcta ctgctggcct tggaacccga aggaaaagcc aataacgaac aactcgcgaa
     6481 aattgcagga cttaaattca acgaaggcct tttgtgcaac gaggaaagcc acggaattcg
     6541 aagaagaaat ccctccgatc atgccatcgt attttccaat cgattctcga cacatccgtc
     6601 ggtcgcgagc ttgcgacgtc tcggcagccg cgccgtgttt tttctcaatg ctggctcgat
     6661 cgacaagcaa gacgacgccg ctggtgaaat cgacttttcc atccaatcgc tccaagggac
     6721 atgggcggac ttgaatggca atttcttgtt cgacgactcc gacgaaacga aaggcgtctt
     6781 tcgactcgtg gcaagtgtga ctcgtcccct cgaagacaag aaaaactcca aggaaaacgg
     6841 cacggaaaac gaaaagcaag aacctgtcga aacgcgaatg gtggtcgtcg ccgatggaga
     6901 tgctctcagc gacgcaaccc tcatgaatcc aagtcacttc aatggcaatc cgcaattcgt
     6961 ggtcgatgtg atccggtggt tgggtggcga agaagctttt tccgggctgg tcgcatccac
     7021 ggaagatgtg cgtatcgagc atacgaagca aaaagataaa gcgtattttt acctgacgat
     7081 ttttggtgta ccggcgcttg tgttggcaat gggatggact ggattgcgac ggaggcgtcg
     7141 cgcgaaggaa gggaggaaag catgaatccc gttcgaagcc tttgggttca cggggctctg
     7201 cttggattgg cctgcctcgg agccgtgatg gtctggacac gcgaccaaca atcgacatcg
     7261 atgagcgtgg cggatgtgcg cgtgtggagc gcaaaaccgg cagaggtgca gaaaatcgtt
     7321 tttgagtcca agtcgaggag cgtggtgctc gaatccaaaa acgatgaggt gggaagatgg
     7381 ttcgagggca cggtgacgcg acaacagatg ccgcggccca agcaagacaa gcaagaggcg
     7441 gatgccacga accacgaccc ggatacgagc gctgtggaca acaaagggga agaaaacggt
     7501 atgcttactg tttttccggt cctgtccgta acaaccgcgg agcgaattgc agaagccgtc
     7561 gcgccactac gtgcgattcg tgaggtggga cacttatccc cagaccaacg acaagacttc
     7621 ggtttggcgg aaccggaagg gatgctgata ctcaccatcg cgggtgtgga gcgaaaactc
     7681 gaggtgggag gcgtggctcc tgggggagcg gaccgttata ttcgtaatcc ggatacggat
     7741 atttcttatg cgatacgggg tgaattctgc cgcgacttgc tctccgggga atccatgctc
     7801 aacgaacggg aattgcacgg tttcgaggag gatgaaatca agcgtgtgcg aatcgatgcc
     7861 aacggcaaat ccagagagat tcttcgtgcg ggagcgggga tgacagcatc ctggacggat
     7921 gcatcaaccc ccgacaagcc tgacgaatcg ctcgccgcgt ggatggccaa agtggcaagg
     7981 cttcgcccca cggagtatct catggctccc cccgcccagg ccgtgcccat catgcggctg
     8041 gaactattcg gaaagaaaga ttccctcggt tttctcgaac ttcttcgatt gcccgacgag
     8101 aaggccggct acgtcgtacg caccgaatac accagaaagc acgcgaaagt gttcccgtcg
     8161 gcaggagaac aagtcgagca agaactcgat tcgcttcttc gccaatagca gttcaggacg
     8221 tcatctctgc agataggcgt cgaaccgtga aaggggatgc aaatctggaa ggaagcgagt
     8281 cttccaaggg ttgaaaatgg gtgacaaatc gctgcgggtt tgtctacggc tgcgacgacg
     8341 ttgccggtgc caccaaaggt tcgagagtac ggcgtatggt tttttgttct tcgtgagaac
     8401 ggtccacgag agtttggcgt ttggtgcgca atagcgtcaa ctgatcttcg gcatcgagaa
     8461 tgcgcttgac gaaaggattg gctgcggctc cggcaccttt ttcgcctcca agagctttga
     8521 gatgttcgcg gaatcgttcg agatcgcttt cgatacgctt catcgcctca tccatatccg
     8581 cgatgagcga tcgcgtttgt tcgagctttt tttgctgctc tttggccgta tcgagcacgt
     8641 ttcgttgcga agcggatagc gtggattgcg tggtcaggga atcgagcctg tcgaacgtga
     8701 tggtatcgag ggaatgcttc tgctgaaaac cttgtataat ccgtacctta cgttccgctt
     8761 tctcatgcgc cggcacgtga aagaccaaga tatacttatc gctttccttg tcatagcgga
     8821 cttcgtcggc accctcgacg ttggcattgt cgacgagcga aagagcccaa tgaaccgctc
     8881 gagattggcc ggagcgattc tccatcaaaa agcgcaggtc ttgggttgtg gtgaagtgtt
     8941 cgaccaacgt gtctttttcc aagacgatca attgtgccgc atgaacctcg gcgtcggttt
     9001 ttttcttcgc ttccacatcg agatccacac caaaaagaag gtaccgctgc tcatggggct
     9061 tgagccgatc caacgtcgat tcacccgcaa atccaccgtc ggaaaacacg gccaccggac
     9121 ccgcgggcaa cgtctgcccc gcgcgattgg tgaacaacac tcccatctgt ggcgcctctt
     9181 gtgagtcaac ccacgtgatg cgcggcgacg ttacagaaga ctgaacgaat ggcacgagag
     9241 cggaaccatg ggcgcgaaga tccacggggc gaggcaagga gtagatgaac aaggccgctg
     9301 actccacgcc ctccgcttgt cgcacggaag ccaagttgcc gacggacagc cgttcgctgg
     9361 cttcgggggg cgattgcgac atggtggctc ccattcgtac agagggtgcg gacgcccgcc
     9421 gggaacctga tagactgcca taaccagcgc cataaccata gcctgtgccg taactctgat
     9481 actcgtcgat atcggcatgg tccccccaga tcccatcggg tgtcctgtcc aacagttgtg
     9541 ggatcgtgga cagatccgtt tcgggcgtga ccaagggtct gcgaccataa cgaggtgcgg
     9601 ccatcgggaa aagaaatgaa tccggcctgc cgttgacgag ttcgacacga acctgttgcc
     9661 aattctcgtc cgtgtcgttg tgtacgagtg cccagccttg caacatcgct tccggcttgg
     9721 tgtcgtcgag gaccaaccgg taggtggttc tccacagcgg agtttccgca atatatccga
     9781 gcgtaaccgg cccttgggca ttcgccatga cgcgaatcaa tcgccgactt tgagatcccc
     9841 tcgtcgacaa tgcattgagc gccaggtcca gacgcgcctg ggtcacgctc gacaaaggac
     9901 gaatcgacgt aatatccgag gcgacgatgt tgaggatctc tttgtttgcc gagagaatca
     9961 gcacgcgaag cgtttgcgtt accggcgttt tggaattgga ttcgtccgag tcgggtgggc
    10021 tttcggaaga gttgggcttt tcggggtttt cttccaccac gatgttgatg attcgtccct
    10081 cgatggtgga cttgggcgtt cgaacgacga cgctttcgcc tttgaggctg gataacaagt
    10141 tcagataggt gatctcggta tcttgggttt tgggaagagc cgccaacgcg cgagccatcc
    10201 cttcggtcac cgaactgtcg aactccaacc cgtctacggt cgtgcggcct ttgccgctca
    10261 cgacaatcag tgttttgagg gcatcgtcga cgtgagccgc gggaaccgga agcgatgttc
    10321 cctcttgcat cgatacctct cctgtccgct cgaaatagcc gacacccgtc tcatagagtc
    10381 ggacggttcg aagaggtaaa atggggcttg gggttttgct cgtgggggtg tttgcgcatc
    10441 ctgtgctgat cgccaacaac cccaggcaga agccgaacca tgggaatcgc ttcattcgaa
    10501 ctctcctcgt gtaagacagg cttggacaac ggtgcggcac ctaggttcct tcctggcttc
    10561 gggaaggata gcgacgagaa atgaatcagg gttcggggtt atccgccagc caggaatgac
    10621 aggcccggct gaagtcgtcg tcgattccga atcgttgtgc ggtgtctcgg cagatgatga
    10681 atgcgcgacg cgctttttca tcgaccggtc gtagtgattc ttgcaaggtt ttccgatatc
    10741 ccgccgcgat gtccggatcc ttggcgatcg atggtggcag gggggattgg gtgacatcct
    10801 gaacgagggc agcccacatt tgcccgattc ggcatgcgga ggcgacgagc caccgtgaag
    10861 gagcaactgg ttgaatttcg gagatttttt ggtaggcctc ttcggctttc agcagtcgtg
    10921 cttgtcgctc acgaacatac gagccgacct tggtctcgag atattcggca acatctttca
    10981 tggcgccaga gccacggtac gcggggaaag gaagaacggt ctgggtccgt aatcgctccg
    11041 ccgaataaaa gtgtgcggcg ccgatggttt cgataccacg ctctcgatcc cacatcggcc
    11101 aaggttccgc tggacccaat tgttgacgcc actgcgatga cggaacggct tgccacaccg
    11161 cgagcgccct gtcgaaagtg gcttgtgcgg atcggttttg tcctgccagc gcacgtccca
    11221 tttccgcatg ccatccggcg gcgactctcg caggttcttc ggggctcgtc gtgcccaaca
    11281 atcgctgcgc gctggcgtgc tcaccctgat cattgagtcg gctggccatg gctacacgca
    11341 tggcggcatg ctcttgctgc tcgctcttgc ttgcgccttt gacgaacgat tccatgtctt
    11401 tttgggcaaa cgaaacgtct ccaagcgcaa ttcgtccgta tgcggccatc cgaacccact
    11461 ctctggcctg agcggcttgg ggttggctcg caaacgcatt ttccgctgcc gaaaccgcag
    11521 tcttcagctc ttgattcgtc ggcgccgtgt ctgtccgcca ccttcgatct cgcgttacca
    11581 ggatggcaaa gatggcgcgg tcgtaccggt cgcttgctcg aaatgaatcg agcatgcgtg
    11641 gggcgacaag ggaagcgcct tcacattgca gatccatgcc gacgtgcgtt tccaggctgc
    11701 agcctcgcca cgccgcaata taggcttcgc ctgctcgatc catggttcga cgacgtgctg
    11761 gttcgtcctc gccttgggct tgttgaaaaa cagcattggc tttgtcaatc aattcgcggc
    11821 attgcgcata acagggcgag ggcgtgcttt cttcggcggc gctgtcgctt cgtccttgcc
    11881 agtcattcga tgaggcttcg tgggcggaag aagaggcggg cggcgaggaa acggtcgatt
    11941 gggccgaacc gcatcccagt gctgtcagaa cgacggcgat ggttcgaaaa gaagatggct
    12001 tcatgaagcg ccactgtatc aagcacacgg caaaagaggg aacgagagtg aaatggcaag
    12061 gaatcaaaac tcgttgtctg ctgttcggtg gagtatcgtt cgcgcatgcg ccttctcgat
    12121 gtttcaagtg cggtagcgcg tcgtgcagtt cggtcaggcg ctcccgtttt cattcccatc
    12181 aaccccatcg agtatcacgg cccacatctt tcccttcata atgactcgtg gataagccgg
    12241 ggcatgatgc acgatctcta ttcgcgcatg ctcagcgagc atcccgagtg ggaatggctg
    12301 gagtttgccg atctggaatg cggcgtgcag ccggttccgg ggccagggtc tcgtggtgtg
    12361 ccgttcgatg tcatgcgacg cctggtgctc gacgtgtgtc gttccgtggc ggagcttgga
    12421 gcgaagcgtg tcgtgctgat gacgtttcac gggagtcctt tgcacggaat cgcattggaa
    12481 cacggagtca aagctttgcg ccgctggggc gtgcatgccg tagcgccgtt caacatcatg
    12541 ctgcgcaagc ttctggatgc tccaggatcg gtgttttcgg atgcttacga tgtgcttggt
    12601 tcgtcttcgg agcgggacat tatgcaagcg gcgtttcaat ccgattttca cggtggtttt
    12661 ttggaaacct ccgtggcgct gcactatgca tcttcgacgg tcgactcgtg tttcgaaaag
    12721 cttccaccgt gtcctgccgt cttgcccgat cgaggtctcg aactggcggc caccatcgca
    12781 agaaaagcag gacgatcgga cttggctcgc gaactacaca tcggtgcgta cggtcaaggc
    12841 tggtacgcat tgcggccgtt tcctggctac actggaaacc cgtcgttggc gacagcagag
    12901 gcgggcaaag catttgcccg aacgttcgtg gagttggcgt ggaaagatgt attgagcgcg
    12961 ctccatgggc gtccttctgc tctcgttcct ctcggatggt ggttcgaata cgcgagcatg
    13021 ggtggaaagc tcagcctgcc gcgtgtcggg ctggacgatg tgttcgttca cgcaccgatg
    13081 gtgagtgaag cagcccacga actgacgtga acgattgccc tctccctttt tgtgcccaag
    13141 gacagaatcg aactgtcgac acgcggattt tcagtccgct gctctaccaa ctgagctacc
    13201 tgggctttct cacaagctat gcttgctgag ggggcgtatc ctaactaagc gtgatcggtt
    13261 gtcaagcgtg atatcaaaag gtgtctgtcg atcacgattg cttccaccga tttagccata
    13321 tcgcaatccc gagggcgagc gtgaccatgg tgccaaatac gacgggagca cgcatggctc
    13381 ggccacgcct ttgcttcgac acggtggcca cgggctcgcg cgcccgttcg atgtcagaca
    13441 gctctaccgt tggcgattcg tcgacggatg cttcctgcga tggatcgtca aaaacaattc
    13501 ccgcctggga atgatcatcg atggttccag gatggccaga aggaggaatg tctccgctgc
    13561 gcgcaaacgt ctagtcgtcg tttcctggac g
//
LOCUS       DAOSOR010000085        13562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4561_length_13562_cov_4.670319, whole genome shotgun sequence.
ACCESSION   DAOSOR010000085 DAOSOR010000000
VERSION     DAOSOR010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 13562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13562
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4561_length_13562_cov_4.670319"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PK710_06640"
     CDS             complement(<1..215)
                     /locus_tag="PK710_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09426.1"
                     /translation="MARPSMPPVSKRPRYLVVALIVAWTFGIGGIMNGCELLRFYKDP
                     NTIPAQVHETTEEGLAGFLAAQQRISK"
     gene            complement(370..627)
                     /gene="rpmA"
                     /locus_tag="PK710_06645"
     CDS             complement(370..627)
                     /gene="rpmA"
                     /locus_tag="PK710_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOT09427.1"
                     /translation="MAHKKGGGSSRNGRDSKAQRRGVKVYGGERIRAGGIIVRQVGNS
                     IHPGKNVGQGRDFTLYALMDGVVAYEWKSNKKKQVSVHPAE"
     gene            complement(638..949)
                     /gene="rplU"
                     /locus_tag="PK710_06650"
     CDS             complement(638..949)
                     /gene="rplU"
                     /locus_tag="PK710_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOT09428.1"
                     /translation="MYAVIKTGGKQYRVAEGESLRIEKVKGEPGDKIVFDHVLMLGGD
                     EPTVGKPTVPGAKVEAQILAQDRAKKIIVFKFKRRKNYRRKQGHRQPYTELQITSISA
                     G"
     gene            1053..2405
                     /locus_tag="PK710_06655"
     CDS             1053..2405
                     /locus_tag="PK710_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09429.1"
                     /translation="MSPRQVHNERIVQGLSVVPALTAILLLISFYFTKLQDVDAAHVL
                     GGPPSVEGIAAWKVVASHYDRGTWLPLSHVPISLETPISDITEGITDDEGAWEARVVL
                     RSQPEQTTLTIRRLDTAAVLLQSPVFLSPKSWNESFRRVAPLVAARQQGPLRIELRLS
                     RATISSSFPEKAWVHVFRDEAPVPKANVVFSGDGISFAPSQHQITDAQGRIEVVFTAT
                     FPSPVLEIHATATDATTGSLQCVLPVRSGTLWIDPQKIDEGILFVASPVHHPFAYITV
                     FDEHSRLLGLRLPLTRTLTGDAIGSMPLGVSPAPHRWVMVSPDPQATGVEPELLGWPL
                     QRSADLPAEAAHVSTAMLGDGKPMALEAARRRSRQGRFRAFMILTLATAIEAMLIGLR
                     SRNANQELESLLAAQPDIDESLRRSLIGGSKRWIHMLIAVSLIAVAFGSFALITWLGI
                     "
     gene            complement(2431..2712)
                     /locus_tag="PK710_06660"
     CDS             complement(2431..2712)
                     /locus_tag="PK710_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09430.1"
                     /translation="MQRIIGMVLVGILSMWMTGCGGAEPPPPLAPDRTAPGVERGSKV
                     TTCRWVPPLPEDREDPCMHDNQGAVLEITERTDMREESKTQQSHTCLCN"
     gene            2910..4172
                     /locus_tag="PK710_06665"
     CDS             2910..4172
                     /locus_tag="PK710_06665"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT09431.1"
                     /translation="MPNIKRCPAQQPADASGKRVFRPTYAVWELTLRCDQCCHFCGTR
                     AGHAHRDELTTAEAKDVIRQLAEMGCKEVALHGGEVYLRPDWLELVRTVREHAMDCTM
                     VTGGKTMSRQQAGQAARAGVSAVSVSIDGLQATHDLLRGVRGSFASATAALTNLAEAG
                     IKIGCNTQVNQKNYHELPQLAEILSHWPLYGWQVQLMVPMGRAANAENLWLHPYDMLE
                     LMPVLGQTRLRADELGIALWPGDNIGYFGTFEHLLRRDRSPKGHSGGCGGGILSIGID
                     ALGEVKGCSAMSSEGFSGGNVRHASLLSIWNDAKELRRARDFHLDDLWGFCRTCYYAE
                     ICKGGCPWTAASVTGRRGNNPYCHHRALEWLRVHKRERLVQVQPAQGANRDTACWNVV
                     LEDAPAAWVAALPEQHPPTPGKREDESM"
     gene            complement(4185..6116)
                     /locus_tag="PK710_06670"
     CDS             complement(4185..6116)
                     /locus_tag="PK710_06670"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT09432.1"
                     /translation="MTHERSTEEIQDAALGESLEGKIVAERYRVMRLLGEGGMGAVYQ
                     AEHVHIRKTVALKVLHKQMCSVPEVVARFEREAVAAGRIEHQNVAVATDFGRLQDGSF
                     FLVLEYAAGRSLRALLQAAGVLEPRRCAHISRQIADALAAAHALAIVHRDLKPDNIML
                     IERDGDSDFVKVLDFGIAKVSLTDMADQPALTRIGAVFGTPDYMSPEQALGQPVDARS
                     DLYALGILMFEMLMGSTPFEHDDPAMVLAHQVTVPAPRLPDHVPSPLASLVMALLEKD
                     VMQRPQSAVEVVQALDRFLRGEASEPDLSGGLSLHGAPIPDKLPSAYGATALDLGKRP
                     DPESSGQVSAVSRLGRAPVGTRSQSQALRSLLSKLDPGLAAGKTRLPLWGILAAAVAL
                     GAVFLLVTTLIAVAVVSKQDAVPVASSGVIPSLSWHPTNMPDPSRSAMRSEIERIESI
                     AVYKRTLDDWVTLARGYSSMDQHRDSALAYRSVLQLDRSRRYDPRLLADLQRAALEPS
                     AFSLVVNLVEGRAGLGETGVDLLWDIWHDLLKHDQPAQAETAFKKLVILSRRASKPLR
                     VAIELHATDRCNKLMEVVSRAEKMADQRSLDRLQHIQSKTECTQEGCYPCLKKSPSLT
                     RAMQRATSHPAPSRDKGYR"
     gene            complement(6364..7950)
                     /locus_tag="PK710_06675"
     CDS             complement(6364..7950)
                     /locus_tag="PK710_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09433.1"
                     /translation="MKSSVIYRFHPVIHRILPSACLLLSALSWADPALPITRCEVLRR
                     AQAWVDDPNAWYSWDPVYTDPTTGVSGYRPDCSGMVSAVWGLPPPGNTTYSFAPGPWD
                     NGVSHVIGASELKPGDALNYPGNPSAGTGHIMLYVSGDFQGGYVEVMEEYNYGHTAER
                     RWRSIDPSIYYPIRYNNIEDGEEEMCHGCNGLIENWGVCNQTPWGALDYAEGDSIYGW
                     AFDGDAGESAIDVHVYCNGPVGSAEAVGVAITADIYRQDLCSVVGSCDHGFRMLTPLS
                     LCDGADHEVHAYAIDVPGGHNPPLSHSPRTLRCEGQLPSGVARWIANPNVYDAWKFSG
                     FHDHLPIDETIVDAVVRGENIPEKPLLVLGPEKDKVYLIDGDMKRHVPDPGVMSAWRF
                     DWNAIQNVDDEQLAGWKEGPSVRERPVVVRDSQGRLLLIDDEIGEPSPKTGGSGGGPD
                     AGVGGGNNSHDGGKEAAAGGAGIGDDNDPKTWWENDTDSGSCAMRPSARNHLEYLIIL
                     WLGAWVGWRGKRSSGRDARR"
     gene            complement(7989..8273)
                     /locus_tag="PK710_06680"
     CDS             complement(7989..8273)
                     /locus_tag="PK710_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09434.1"
                     /translation="MNRSLNSKPAPRKLENDTATVFSFPNLPIMVRVPRWRASVSPRV
                     LQQIRVRGGSDPSPRGKKIRLGTDGWSRAMPRAWIPFRWMVWNMQVNQVL"
     gene            8277..10289
                     /locus_tag="PK710_06685"
     CDS             8277..10289
                     /locus_tag="PK710_06685"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT09435.1"
                     /translation="MRQSSISGRWVLTLALWVGAVHGLGCASDAEPEPVPATGGSAGA
                     GGSGATGGVSGEAGSAGAGATGGAGGATGGMGGSGGATGGTGASGGIGGSGGATGGTG
                     ATGGSGASGPDATIQVGAPDRFLLSGMIVTPDTTFEGQVLAEGDTLTCVKPGTECANE
                     AGAQGATIIDTHGIIAPGLIDTHNHILFDIFGDEHWLPNIPSTCSNASDCAASSYCSN
                     NKCNCVEGTCKYQNHNQWPNEREYGVMMDYKQCLEDASQGKPVWCPQTYKGNGKVKCE
                     MDKWGELKGLIAGTTSIVGLPGTSSACFSSLSRSIDVAQNGLPSDKVQTSATFPPSTS
                     SADGVCKNYSSGATEAYLIHCGEGVDATALKEFSTLYTVTTDDGCLYAPGTAITHGTS
                     FTSAEYETMAKAGMKLTWSPASNVALYGSTNDLPAAIAAGLTISLAPDWSMGGSRNML
                     EEMRFANQWDDTHYGNVLSTKDIVEMSTSNAAAVIALSNTLGRLTPGFKADLFVVGGD
                     VSLPYDAIVSAWPSTVRLVMVGGKVLYGDDQLEAAGPMNPGCEAIDVCGRAKFICVAE
                     ASSKDLLDQTLVEIRDRLNNALLDLDSIPVLPASSCKPACGANEECYERTVFAEADPS
                     KCGAPCGLGEKCYQKTASTYGCMPTAACSPKREKKFHPVTPLFACP"
     gene            complement(10301..12157)
                     /locus_tag="PK710_06690"
     CDS             complement(10301..12157)
                     /locus_tag="PK710_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09436.1"
                     /translation="MIDGLFENPPPFETDLVMRLDKVDKRLARQCLVERLRGSQPTEQ
                     ETGIYMAAFGQLGLVEQCAAMAEIILDGRMNHRIRAYACGLVASEDRSLAEETMTMLP
                     AHERLAIMDVPYIDLLVWAQADPSAGEQLAWALDSDVMGMTFDRLEWHRKRVGTGAVS
                     AYGAALRNKQACSYHRGMLDALVAESSPEAFVLLRSLRDEETDPALRRDYQAALLRMG
                     TRAIDPSITREIPKGRAFVSSCDHLGDFVILGCMDNPDGSVSIANLCIRADAQIRGGF
                     VLLRQTQQDLQEIFEELDRTGGGFAPLSLQEGARLVDEALQRMEARGQKVARDVEPAI
                     ALFRRASRRKRKSTPDISPALDITMEQVRELMRRPEHARTWILVPLDFEAIRALPPPS
                     HRLDEAWIREAAALWARRGKPRKRILSVVRHMAFWHDLRGEQQQASLCSGLARSMEKQ
                     AATSPLVLGLFEKAFMFPKYEPTSLSETITRNYIRKALFSGIRIAKGRDLARLDFIHI
                     AFIFLSRTLTHVDPVPLPKDILLTIADGLATAYVDRVIGDKKRTAGKKSEDLLASCPP
                     AVEDIKNDIVSNLDDFLHEVCAICPIRCIDKPKARMTELFFRDKHPALLDSE"
     gene            complement(12266..13243)
                     /locus_tag="PK710_06695"
     CDS             complement(12266..13243)
                     /locus_tag="PK710_06695"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT09437.1"
                     /translation="MALSILSDFEISLPKKPGVALWQGMTCIASNEVSCSDDMRTVRL
                     TIDGLAYVLMSGLVVGCGANSSFDTTDASVDGEDSGGCLPGPDGGTTADSQPDSGGKD
                     SAKDAVIDVDPDREWLDCPSDMVAVGPVCVDRYEASRSDATAMDQGVAVGPAHSVPGV
                     IPWHVDLMTSEALAEFEAACVAANKRICRPEDWRAACEGATGSTYVYGNVFDARTCNC
                     VDTYCADWCLEHGVEPENCDTGANCGYVHDSFHLMPTGSFPMCTNELGTFDMTGNVWE
                     VVPSKVDVRGFEVRGGAFNCAGAIDRLTCTFNATWAQLFAGFRCCLDRI"
BASE COUNT         3036 a         4006 c         3722 g         2798 t
ORIGIN      
        1 tctttggaaa tgcgttgttg tgccgcgaga aatccggcca aaccctcttc cgtggtttca
       61 tgaacctgag cggggatggt attcggatcc ttataaaacc ggagcaactc gcaaccgttc
      121 atgatgcctc cgatgccaaa cgtccacgcc acgatcaaag caaccaccag gtatcgtgga
      181 cgttttgaaa caggaggcat cgaggggcga gccatggata tctcgaactc gggatattcg
      241 aatccgtcaa tcgctcgtca gagcgaatgc gtagggtaag tgtcccttag cacggcatga
      301 gcgatccgtt catgcccaaa cgcggaaagt gcagcgaaag gcgagagaat cgcaaacaga
      361 ggggcaagcc tattcggcgg gatgaaccga cacttgcttt ttcttattgc ttttccactc
      421 gtaggcaacg acgccatcca tcaaagcata gagggtgaag tcgcgacctt ggccgacgtt
      481 tttgccaggg tggatgctgt tgcccacttg gcgaacgatg atgccacccg cacggatgcg
      541 ttcgcccccg taaaccttga caccacgtcg ctgcgctttg gaatctcgac cgttgcggga
      601 agaaccgcca ccttttttat gcgccatggc tctctcctta tcccgctgag atgctcgtga
      661 tctgcagctc ggtgtagggt tgacgatgtc cttgcttgcg tcggtagttc ttgcgtcgct
      721 tgaacttgaa gacgatgatt ttcttggctc gatcctgcgc tagaatctgg gcttcgactt
      781 tggcaccggg cacggtaggt ttccccaccg tcggctcatc gccaccaagc atgagaacat
      841 gatcgaatac gatcttgtct ccaggctctc ccttgacctt ctcgatacga aggctttcac
      901 cttcggcgac gcggtactgt ttaccgcccg ttttgattac ggcatacatg gttactcacc
      961 ggtaaaattc aggacggcgg agtatagatg cacatgggac gattgcaagc ccgaagtaac
     1021 cgacacgaat ctacacgaaa aaaggccgga tcttgagtcc ccggcaagtc cataacgaac
     1081 ggatcgtcca agggctatcc gtggtgccag ccctgacggc gatccttcta ttaatttcct
     1141 tttatttcac taagttacag gatgtcgacg cggctcatgt cttggggggc ccccccagcg
     1201 tcgaaggtat tgccgcgtgg aaagtcgtcg catcccacta cgaccgaggt acctggctgc
     1261 ccctatccca cgtgcccatt tccctcgaaa ctcccatctc cgacatcacc gaagggatca
     1321 ccgatgacga aggcgcttgg gaagcaaggg tcgttttgcg ttcacaaccg gaacaaacca
     1381 cgctgaccat ccggcgcctc gatacagcgg ccgtcctgct tcaaagcccc gtgtttcttt
     1441 ccccaaagtc ctggaacgag tcgtttcgtc gggtcgcacc cctcgtggcc gcaagacaac
     1501 aaggaccctt gcgtatcgag ctgcgtctga gccgggcgac gatttcctca tcgtttcccg
     1561 aaaaagcgtg ggtacatgtt tttcgcgacg aggcccccgt gcccaaagca aacgtcgttt
     1621 tttcaggaga tggtatctcg tttgcgccgt cgcaacacca aatcacagat gcgcaaggac
     1681 gaatcgaagt cgtcttcacc gctacatttc cttctcctgt cctggagatc catgccacgg
     1741 ccaccgatgc caccacgggt tcgttgcaat gcgtgttgcc cgtccgctcc ggaactctat
     1801 ggatcgaccc gcaaaaaatc gatgaaggaa tccttttcgt cgcatcaccg gtccaccacc
     1861 ccttcgcgta catcaccgta ttcgatgagc attcgcgcct gctcggcctg cgtttgccgc
     1921 ttacgcgaac actgacgggc gatgcgattg gctcgatgcc tcttggcgtt tcgccagcgc
     1981 cccatcgttg ggtgatggta tctcccgacc cccaagcaac gggggtagag cctgagttat
     2041 tgggttggcc gctgcaaagg tccgctgatc ttcccgccga agccgcgcac gtttcgacgg
     2101 cgatgctagg ggacggcaag ccaatggctc ttgaagctgc acgacggcga tcgcggcaag
     2161 gccgttttcg cgcttttatg atcctcaccc tcgccaccgc catcgaagcc atgctcatcg
     2221 gccttcgttc acgcaacgcc aatcaagaac tcgaaagctt gctcgccgcg caacccgata
     2281 tcgacgaatc gcttcgccgc agcctgatcg gagggtcgaa acggtggatc catatgctga
     2341 ttgcggtatc gctcatcgcc gtggcatttg gctcgttcgc attgatcacg tggctgggaa
     2401 tctgaagggg gctcgtgctt cgtggcttca tcagttgcaa agacacgtat gggattgctg
     2461 tgtcttgctc tcttcacgca tatccgtacg ctctgtgatt tccaacaccg ccccctggtt
     2521 atcgtgcatg cacgggtcct ctcgatcttc gggcaacggt ggcacccaac gacaggtcgt
     2581 tactttcgac cctcgttcga cgcctggtgc cgttcgatcc ggtgccaatg gcggcggtgg
     2641 ttccgccccg ccacatcccg tcatccacat cgaaagaatg ccaaccaata ccatcccaat
     2701 gattcgttgc atgggttcct cccgctcaga tcatgcagcg gatgccttgt cgcccgtctg
     2761 ttatccttca ccccacggta tgacgcaagt tgtcgcgcac gcttccgttg ggatatggca
     2821 aaaagcgcat ctatcgcgca ctcccacgcc gcgacccgct ttccaccttc tgctcgtttc
     2881 cgtatgagtt aggatccccg accatgggca tgcccaacat caagcgctgt cctgcccagc
     2941 aaccggccga cgcctccggc aaacgcgtgt ttcgccccac ctatgccgtc tgggaattga
     3001 ccttgcgctg tgaccaatgc tgccacttct gcggcacccg cgcgggacat gctcatcgcg
     3061 acgaactgac tacggccgaa gccaaagacg tcatccgaca gcttgcggaa atggggtgca
     3121 aggaagtcgc tcttcacggt ggcgaggtct acctgcgccc tgattggctc gagttggtgc
     3181 gcaccgtacg tgaacacgcc atggattgca ccatggtcac ggggggcaag acgatgagcc
     3241 gacagcaagc cggacaagcg gctcgagccg gtgttagcgc ggtcagcgta tccatcgacg
     3301 ggctacaagc cacccatgac cttcttcgag gagtacgcgg cagctttgca tcggccacgg
     3361 cagccctcac gaacctggcc gaagcgggga taaaaatcgg ttgtaacacg caggtcaatc
     3421 aaaaaaatta tcatgaatta ccacaacttg cggagattct gtcgcattgg cccctgtatg
     3481 gttggcaagt gcaactcatg gtgccgatgg gtcgcgcggc caatgcggag aacctttggc
     3541 tgcatcccta cgacatgctc gagctgatgc ccgtattggg acaaacacgc ctacgcgccg
     3601 acgagcttgg aatcgctctg tggccggggg acaacatcgg atactttgga accttcgaac
     3661 acctgctgcg tcgcgatcga agccccaaag gacactccgg cgggtgcgga ggcggaatcc
     3721 tgtcgattgg catcgatgcg ctgggggagg tcaaaggttg ctcggcgatg agttcggaag
     3781 gattttccgg aggcaatgtg cgtcacgcat ccctgctgtc gatctggaac gacgcaaaag
     3841 aactccgcag ggctcgtgac tttcacctcg atgatttgtg gggattttgc cgtacgtgct
     3901 actacgccga aatctgtaaa ggcggatgcc cctggaccgc ggctagcgtg acggggcgta
     3961 gaggcaacaa cccgtattgt catcaccgtg cgctcgaatg gctccgcgtc cacaagcgcg
     4021 aacggctggt ccaagtccaa ccggcccaag gcgccaaccg cgataccgct tgctggaacg
     4081 tggtgttgga agacgctcct gcagcatggg tagccgcgtt gcccgagcaa catcctccca
     4141 cgccgggcaa gcgcgaagat gaatctatgt aacctgttga tatttcaacg atatccctta
     4201 tcacgggagg gtgcgggatg gctcgtagct cgttgcatgg cgcgcgtcaa actcggtgat
     4261 ttcttcaaac aagggtagca accttcctgc gtgcattcgg tcttcgattg gatgtgttgc
     4321 aacctgtcca gggaacgctg atccgccatt ttttccgcac ggctcactac ctccatgagt
     4381 ttgttgcaac gatccgtggc gtgcaattcg atcgccacac gaaggggttt cgaagcgcga
     4441 cggctcaaaa tgacaagctt tttgaaggca gtttcggcct gagcaggttg atcgtgcttc
     4501 agcaaatcgt gccaaatatc ccaaagcaaa tcgactccgg tctctccaag acctgcccgg
     4561 ccttccacaa gattgacgac caaagaaaat gcgctgggct cgagggcggc gcgctgaagg
     4621 tccgcgagca gtcgaggatc gtagcgtctt gaccgatcga gttgcagaac ggatcgatac
     4681 gcgagcgcac tatctcgatg ctggtccatg gacgaatatc cgcgagccaa cgtgacccaa
     4741 tcgtcgaggg tgcgcttgta cacggcaatg ctttcgatgc gctcaatctc ggatcgcata
     4801 gccgaacgcg atggatcggg catgttcgtg ggatgccaag acaacgaagg aatcacaccg
     4861 gaagaagcca cgggaacggc atcttgtttg gacaccacgg ccacggcgat cagggtcgtc
     4921 accagcaaaa agacagcccc caacgccacc gcagccgcca aaatccccca caacggaagg
     4981 cgggtctttc ccgcggccaa tcctggatca agcttcgaca atagacttcg gagcgcttgg
     5041 gactgagatc gagtgccgac cggcgcgcga ccaagccggg aaaccgcaga gacctgcccc
     5101 gaggattccg ggtccggacg cttacccaag tccaacgccg tcgccccata agcggaggga
     5161 agcttgtcag gaatcggggc tccgtgaaga gatagacccc cggacaagtc tggttcgctc
     5221 gcttccccac gcaaaaaacg atcgagagcc tggaccactt cgacagcgga ttgcggccgc
     5281 tgcatcacat ctttttcaag gagcgccatg acgagcgacg cgagaggaga cgggacgtga
     5341 tcgggcaggc gcggagcggg aaccgtaact tgatgcgcaa gcaccatcgc tgggtcatcg
     5401 tgttcgaatg gggtggaacc catcagcatt tcgaacatca agatccccaa cgcataaaga
     5461 tcgcttcgcg catcgacagg ttgcccgaga gcttgctcgg gagacatata atccggggtt
     5521 ccaaagacag cgccgattcg tgtgagagcg ggttgatccg ccatgtccgt gagcgaaacc
     5581 ttggcaattc caaagtccag gaccttgacg aagtcggagt ccccatcgcg ttcgatgagc
     5641 atgatattgt cgggcttcaa atcccgatga acgatggcaa gggcatgcgc cgcggcaagg
     5701 gcatccgcga tttgtcggga gatatgcgcg cagcgacggg gttcgagcac gccggcagct
     5761 tgcaacaagg cgcgtaaact gcgaccggcc gcatactcca agaccagaaa aaaagagcca
     5821 tcttgtaggc gaccaaagtc ggtggccact gccacgttct ggtgttcgat tcgacccgcc
     5881 gcgaccgctt cccgttcaaa gcgcgccacc acttcgggca cactgcacat ctgcttgtgc
     5941 aacaccttga gagcaaccgt ctttcgaata tgaacgtgct cggcttggta caccgccccc
     6001 atgccgcctt cgccgagcaa cctcatgact cgatagcgtt cggcaacgat ctttccttcg
     6061 aggctttccc ccaaagcagc gtcctgaatc tcttcggtcg atcgctcgtg agtcacatct
     6121 ccacggatag ccgagggatg gataaggcac aacgatcggc aacgaattga gcgtctggtc
     6181 cctaggaagg gaaaaacccg tgccctgttc gaactccctg gcccgctttc gtccatcgga
     6241 accatcgaga gtcacccgcc agcgaattcg aggtgacgat gagggtcaat cgagcacgca
     6301 tcaaagcacg atcacactct tcgcacttcc gaaagacaac cgctgccaca aggcgcacca
     6361 cattcaccga cgagcgtcgc gcccactcga gcgtttgccg cgccatccaa cccacgcgcc
     6421 aagccataaa ataatcaaat attccaaatg gttacgagca gaaggacgca tcgcgcagga
     6481 gcccgagtcc gtatcgtttt cccaccacgt tttcggatca ttgtcatctc caatcccggc
     6541 gcctcctgcc gcagcttcct tcccgccatc atgggagtta ttgccgcccc ctacacccgc
     6601 atccgggccg cccccagaac caccggtttt cggcgacggt tccccgattt catcgtcgat
     6661 caacaacagc cgcccttgcg aatcccgcac cacgacaggc cgctcccgca cgctcggccc
     6721 ctctttccac ccggccaact gctcatcgtc gacgttttgg atggcattcc agtcgaagcg
     6781 ccacgcgctc atcacgccgg gatcagggac atggcgcttc atgtcaccgt cgatgagata
     6841 gaccttgtcc ttttcgggac ccaaaaccaa caacggtttt tcagggatat tctcgccgcg
     6901 taccaccgca tcgacgatcg tttcgtcgat gggcaggtgg tcgtggaagc cggaaaactt
     6961 ccacgcatcg taaacgttgg gattggcaat ccaccgtgcg acccccgaag gcagttgtcc
     7021 ttcacaacgg agggttctgg gagaatggct caagggcgga ttgtggccgc ccggaacatc
     7081 gatggcatag gcatgtacct catggtctgc gccatcgcac aagctgagag gtgtgagcat
     7141 tcgaaacccg tgatcacagg agccaaccac cgagcataag tcttgtctgt agatatcagc
     7201 ggtaatcgct acccccaccg cttcggcact tcccaccggg ccattgcaat aaacatgcac
     7261 gtcgatcgcc gactccccgg catcaccatc gaaagcccaa ccgtagatag aatcgccttc
     7321 ggcataatcc agcgcgcccc atggggtttg gttgcatacc ccccaatttt cgatcaatcc
     7381 gttacagcca tgacacattt cctcttcgcc atcttcgata ttgttgtaac ggattggata
     7441 ataaatggat ggatcaatgc ttcgccaacg ccgctcggcc gtatgtccat agttgtattc
     7501 ctccatgacc tcgacgtaac cgccctggaa gtctccggat acgtagagca tgatatgacc
     7561 cgttccggca ctcggattgc ccggatagtt gagcgcgtcg ccgggcttca gctcactggc
     7621 gccaatcaca tgagaaaccc cattatccca gggtcccgga gcgaagctat aggtggtatt
     7681 gccgggagga gggagtcccc atacggcgga aaccattccg gagcaatccg ggcggtagcc
     7741 agacactccg gtcgtggggt cggtatacac cggatcccat gaataccagg cgttgggatc
     7801 gtcgacccag gcttgcgccc gacgcaacac ttcgcatcga gtgatgggca aagcggggtc
     7861 agcccaggat agggcggata ggaggaggca ggcggagggt aggatcctgt ggatcacagg
     7921 gtgaaatcga tagatcacac tggatttcat ggcggaaggc caatggcggg gcgacagtgg
     7981 caggcctttc ataaaacctg attcacttgc atgttccaca ccatccatcg aaagggaatc
     8041 cacgcacgag gcatggctct ggaccaacca tcggttccaa ggcgaatttt tttgccgcgt
     8101 ggagacggat ccgaaccacc acgaacgcgg atctgctgca aaacgcgagg ggaaaccgac
     8161 gcccgccagc gcggaacccg aaccatgatc ggcaagttgg ggaacgaaaa gacggtggcg
     8221 gtatcatttt ccaactttcg tggagcgggc ttcgaattga ggctacgatt cataggatga
     8281 ggcagtcttc catttcgggt cgttgggtgc tgacattggc tttgtgggtg ggcgcggttc
     8341 atgggttggg ctgtgcttcg gacgcagagc ctgaaccggt tccggccacg ggaggctcgg
     8401 cgggagcggg cgggagcggg gccaccggcg gtgtctctgg tgaggcggga agcgcgggtg
     8461 ccggagcgac aggcggcgcg gggggggcca cgggaggaat gggcgggtcg ggcggggcca
     8521 ccggaggcac gggcgcatca gggggaatag gaggttcggg aggtgctacc gggggaactg
     8581 gcgctacggg cggttcaggc gcgtcgggtc ctgatgctac catccaagtc ggagcgccag
     8641 accgattcct gttgtccggg atgatcgtga ccccggatac caccttcgaa ggtcaagtgc
     8701 tcgccgaagg cgacacgctc acctgcgtga aaccgggaac cgaatgtgcc aacgaagcag
     8761 gagcgcaagg agccacgatc atcgacaccc atggcatcat cgcgccaggg ttgatcgata
     8821 cccataacca tattctcttc gatattttcg gcgacgagca ttggttaccc aacatccctt
     8881 cgacatgctc gaacgcaagc gactgcgcgg cttcgagtta ttgctcgaac aacaaatgca
     8941 attgtgtgga gggaacttgc aaatatcaga accacaacca gtggccgaat gaacgcgaat
     9001 acggcgtgat gatggactac aagcaatgcc tcgaggacgc ttcccagggc aaaccggtat
     9061 ggtgtccaca aacgtataag gggaacggca aagtcaaatg cgaaatggat aaatggggag
     9121 aactcaaagg gttgatcgcc ggaacgacat ccatcgtcgg actgccagga acctcatcgg
     9181 cctgtttttc gtcgctttct cgatcgatcg acgtggctca aaacggcttg ccgtcggata
     9241 aagttcagac gagtgcgacg tttccccctt cgacgagttc ggccgatggg gtttgtaaga
     9301 attattcatc gggagcgacg gaagcgtatc tcattcattg tggagaagga gtggacgcca
     9361 cggcgctcaa ggagttttcg acgttgtaca cggtaaccac cgatgatggt tgtctttatg
     9421 cgcctggcac cgcgataacg catggtactt cgtttacgtc ggccgagtac gaaaccatgg
     9481 caaaagcggg tatgaaactc acgtggtcac cggcttccaa cgttgcttta tacggttcga
     9541 ccaacgattt gccagcggcg atagcggcgg gattgaccat cagcttggca ccggattggt
     9601 ccatgggggg aagtcgaaat atgctcgagg agatgcgttt cgccaatcaa tgggatgata
     9661 cgcactatgg caatgtactg agcaccaaag acatcgtgga aatgtcgaca tcgaacgcgg
     9721 cagccgtcat tgccttgtca aacacgttgg ggcgtctaac ccctggcttc aaagccgatc
     9781 ttttcgtcgt gggaggcgat gtttcgctgc cctacgatgc catcgtgtcg gcgtggccga
     9841 gtacggttcg gttggtgatg gtaggaggca aggtgctgta cggtgatgat caactcgaag
     9901 cggccggtcc catgaatccg ggttgtgaag ccatcgacgt ctgtggtcgg gcgaagttca
     9961 tttgcgtggc agaagcgagt tccaaggact tgctggatca aacgttggtg gagattcgcg
    10021 accggttgaa caatgcattg ctcgatctcg attcgattcc ggttcttccc gcttcctcct
    10081 gcaaaccggc ttgtggcgcc aacgaagagt gctacgagcg aaccgtattt gccgaagcgg
    10141 acccaagcaa atgcggggct ccctgtggct tgggagaaaa gtgttatcaa aagacagcca
    10201 gcacgtatgg ctgcatgccg acggccgcct gttcaccgaa acgcgaaaag aagtttcatc
    10261 ccgtaacccc cctgtttgcc tgtccgtaac acacgcgacg tcattcggaa tccaaaagcg
    10321 cgggatgttt gtcgcgaaaa aacaattccg tcatacgggc tttgggtttg tcgatgcatc
    10381 gaataggaca gatggcgcaa acctcgtgta gaaagtcgtc gaggttggaa acgatatcgt
    10441 ttttgatgtc ctccacggcg ggaggacacg aagccagcaa atcctcgctc tttttccccg
    10501 ccgttcgttt tttatctccg atcacacgat cgacataagc ggtagccagc ccatccgcaa
    10561 tggtcagaag aatgtccttg ggcaacggta cgggatcgac atgggtcaac gtgcgactga
    10621 ggaaaatgaa ggcgatatgg atgaaatcga gccgagccag gtcgcgtcct ttggcaattc
    10681 ggatcccgga gaacaacgct tttcgaatgt aattgcgggt gatcgtctcc gataggctcg
    10741 tgggctcgta tttcggaaac ataaacgcct tctcgaacaa accgagcacc agaggactgg
    10801 tggccgcttg tttttccatg ctacgcgcaa gccccgagca taacgaagct tgctgttgtt
    10861 ccccgcgaag gtcatgccaa aacgccatgt gacgcaccac cgaaaggatg cgctttcgtg
    10921 gcttgccgcg tcgcgcccac aacgcggcag cttcacgaat ccacgcttca tccaaccgat
    10981 gcgaaggcgg tggaagcgca cgaatcgcct cgaagtccaa ggggaccaat atccatgtcc
    11041 gcgcgtgctc aggccttcgc atcaactcac gcacctgctc catcgtaata tcgagggctg
    11101 gagaaatgtc gggagtcgat tttctttttc ggcgagaagc gcgacgaaat aacgcgatgg
    11161 caggctcaac gtcacgcgcg actttctgtc ccctagcctc catacgctgc agcgcctcgt
    11221 cgaccaacct ggccccctct tgcaaagaca aaggagcaaa accgcctccc gttcggtcca
    11281 attcctcaaa aatttcttga agatcctgct gcgtttgacg aagcaacacg aaccccccgc
    11341 ggatctgcgc atcggcgcgt atacaaaggt tcgcaatcga tacggaccca tccgggttgt
    11401 ccatgcagcc aaggatgacg aagtccccga gatgatcgca gctcgagacg aaggctcttc
    11461 ccttcggtat ttcccgcgtg atcgatggat cgatggcacg ggtccccatg cgcagcaatg
    11521 cggcttggta atcgcgacgc aacgccggat ccgtttcctc atcccgcagg gaacgaagaa
    11581 gtacaaacgc ttctggagag gattccgcca cgagcgcgtc gagcattccg cggtgatagg
    11641 agcaggcttg tttgttccgc aacgccgcgc cataagcact gacggctcca gtgcccaccc
    11701 gttttcgatg ccactccaaa cgatcgaacg tcatgcccat cacatcactg tcaagcgccc
    11761 acgcaagctg ttcgcccgca ctcggatcgg cctgagccca caccagcaga tcgatatacg
    11821 gcacatccat gatcgctaac cgctcgtgtg ccggcagcat ggtcatcgtt tcctcggcaa
    11881 gagaacggtc ttcggaagcc acgagcccac aggcataggc tcgaattcta tggttcatcc
    11941 gaccatcgag gatgatctcg gccatcgcgg cgcattgttc gaccaacccc aattgcccaa
    12001 aagcagccat gtagatgccc gtctcttgct cggtgggctg agaaccacgc aagcgctcga
    12061 ccaaacactg acgagccaga cgcttgtcga ctttgtcgag ccgcatcacc aaatcggttt
    12121 cgaagggtgg tggattctcg aacaaaccgt cgatcacggc tctcgtggaa tcagccccgg
    12181 atggttcgtc ctcagaacgt cgtgaccgtg atcgggaagg cacaaagcca tcgtatcatc
    12241 catgcccgag gtcgcgtaca caggatcaga ttcggtcgag acagcaacga aagccggcaa
    12301 atagctgagc ccaagtcgcg ttgaacgtac atgtgagccg gtcgatggca cccgcacaat
    12361 tgaatgcgcc cccccgcact tcgaatccac gaacatccac tttcgacgga accacttccc
    12421 acacgtttcc ggtcatgtcg aaggttccca attcattcgt acacatcgga aatgatcccg
    12481 tgggcatgag atgaaaactg tcatggacat aaccgcagtt ggcacccgta tcgcaatttt
    12541 ctggctcgac accatgctcc aaacaccaat ccgcacaata agtatcgaca caattgcacg
    12601 tgcgagcatc gaaaacgttg ccgtaaacgt aagtggaccc ggtcgccccc tcacaggccg
    12661 cacgccaatc ttccggacga caaatccttt tgtttgccgc cacacacgca gcctcgaatt
    12721 cggcaagcgc ttcggaagtc atcagatcca catgccaggg aatgaccccc ggcacactat
    12781 gggctggccc caccgcaact ccttgatcca tggcggttgc atccgaccgt gaggcctcat
    12841 accggtcgac acatactggc ccgaccgcca ccatatcact tggacaatcg agccattcac
    12901 ggtccggatc cacatcaata acagcgtctt ttgcactgtc ttttccgcca gaatccggct
    12961 gcgaatcagc agtcgttccc ccatctggac caggtagaca accgccacta tcctcaccat
    13021 ccacactcgc atccgtagtg tcaaaactcg aattggcccc acaacccacc accaaaccgc
    13081 tcatcagaac atacgctagg ccatcgatgg tgagtctcac tgtccgcatg tcatcggagc
    13141 atgaaacttc gttcgaggcg atgcaagtca tcccttgcca caaggcaaca ccgggttttt
    13201 tcggcaacga gatctcgaag tcggaaagga tcgataacgc cactttggta tagcaggtcg
    13261 ttgttctttc ccttcactta ctcaaattca acgcgagtgt tccgaaacga ggagacgctc
    13321 gcaatcgact tcctcgagca aagttcaact aaaaatggag acaggtcaaa cggcgctacc
    13381 atcgcgttgg gtctcgccat tggctggagt tcatttgttc tacctcgggg gtcgactttg
    13441 gcaaaattca tatgcttagg ggttcaacgg gctcaaggat tcctcgttat caaaatcaat
    13501 cttgcactca acgtaggttt cgcgcccctt caattcgttg agtggcagtc cagcgcgata
    13561 at
//
LOCUS       DAOSOR010000086        13508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4581_length_13508_cov_4.346168, whole genome shotgun sequence.
ACCESSION   DAOSOR010000086 DAOSOR010000000
VERSION     DAOSOR010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 13508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13508
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4581_length_13508_cov_4.346168"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PK710_06700"
     CDS             <1..227
                     /locus_tag="PK710_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09438.1"
                     /translation="VKCRITGLYLRSGVFEVLPVDDKIRRMIATSVSSKEIMKQARQD
                     GLLTLREAAIRKMAKGVTSFEEVLRVTVDG"
     gene            224..1672
                     /locus_tag="PK710_06705"
     CDS             224..1672
                     /locus_tag="PK710_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT09439.1"
                     /translation="MKAERSIERFARKLRRYWEAGYPIVWLVTYEEQRAIEVLRRLAP
                     SWPIQIWSWSGRQAEDDLDEMLAKAASSESPGLNVLLDVPTRWAEGRMIRALRNFAAS
                     GRPGRHVIIVSPSLELPVELEKDVCVLDFPLPDEVDLRAAVRGEAQRKGIGNFDEEHL
                     ARCAGGMTWLEAQRAFRMALSVQDPSQSARLVLSEKRQVMRRSAALELVEPEVDLQDV
                     GGLEVLKGWLRTRVVACGPEARAFGLPEPRGMMVCGVQGCGKSLVAKATASVFGLPLV
                     RLDFAAVFASSSPELAVRQATRMAEAIAPVVLWVDEIEKGLGQGTLESKNARVFGDFL
                     IWMQEKGAPVFVAATANEVEQLPPELVRRGRFDEVFFVDLPSARERADILAIHLRRRG
                     RHAERYPLEELVKPWDYVSGAEIEQVVIAALFRAFAAKRELEAEDIRLAGGELVPLAT
                     MYEERVQALRTWATSRARKASTDRRTLELFED"
     gene            1739..2926
                     /locus_tag="PK710_06710"
     CDS             1739..2926
                     /locus_tag="PK710_06710"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOT09440.1"
                     /translation="MNLGPGEKTILLMAGFVIVVAGLRFGEPLLVPLFLALVIATVSS
                     PIIRWLEKRRVPSALAIILAIVLDLAVLAALGTLVGSSLSDFSEAAPRYQAALSNLIK
                     TNVAWLAARGVPASSDLLAPLQDFGRLVDLLGGLVKSVANVVSMLLFVLLLVVFMLVE
                     FGRWQLKIGYALGDRHADLSRFGNAARELQKYLGVKTAANAVTGLLCGVWAAIMGVDF
                     PILWGLIAFLLNYIPTIGTIIAAIPPIVLAWLAFGPGTAFVTAVGFVVINVTLGNVIE
                     PRIMGRALGLSPLVVLISMVFWWWLWGPVGALLSAPLTMMVKIMLANTTDLRWLAIML
                     GSPSWVEEMQAQWDGARPVEHKSELCVRSEKPAHEVLLGVRATDPPRDPSATPYAIPR
                     SPE"
     gene            3104..4075
                     /locus_tag="PK710_06715"
     CDS             3104..4075
                     /locus_tag="PK710_06715"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOT09441.1"
                     /translation="MPVSSPTPDAHAPPRDQRPWWRRALPFAIALGLLSLLISRLDLA
                     MFARHLRQVNTVGFLAFCFVFSITLLASDSLATSFVYSRTVCPVRFHEVFLIRGASYL
                     PSMLNHHLGQAWLTWYVSRVYKAPLGRVAGATLLVYGTTFGCLLLLGGVSLALDASSA
                     RWLGPIMIAASVGAVVYLVVIASRPAILVRRQVLAPLFDVGVVGHLLAFALRVPHILV
                     LFLGTWLSFRFFDVDIPPAVALVTMPVLLVISALPVTPQGVGTRDWFSLHAFSQYAIG
                     DKMEQEAAVAATTLSFAVGISIFQAALALYLMHRALRVLAMTKDKAS"
     gene            complement(4086..4766)
                     /locus_tag="PK710_06720"
     CDS             complement(4086..4766)
                     /locus_tag="PK710_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOT09442.1"
                     /translation="MHPIWFFSIPLFAIGVGTACSSEDSPTPFNDVDVVGTDRNPEGI
                     PYPSDDWGARPRDGNKPGQRLPNFSFQGYPDSRKDDGLQVVSMADYFDPSQQHHKLMF
                     LMVVVAWCPHCQAETRAVVKLNDSFRANGIRVVQTIVEGKRRGSPLSLADVDDWVDTM
                     NTPFSVLIDVNAKRLGTVATIRGVPWNALIDTRSMEILSIAEGELAYPASYANDGLNW
                     VATHEPRP"
     gene            complement(4836..5771)
                     /gene="thiL"
                     /locus_tag="PK710_06725"
     CDS             complement(4836..5771)
                     /gene="thiL"
                     /locus_tag="PK710_06725"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOT09443.1"
                     /translation="MAGEFERIEHLRRLFGPAPEGVSVGIGDDAAEIDVPVGEKIVWT
                     IDACIENVHFRQDIASWEDVGFRSMMAAASDLAAMAATPIGALCSVSVPRNLPDDALY
                     ALANGQKLAAQQLGSSVIGGNLSASQIVTVSTTWLGRTTLAPRRHGARPGDQLALCGP
                     VGFAALGLRMLLKKPSWTAADEIETIALHAWRRPRARIEEGLRCMHASSLIDISDGLA
                     QDALHLAMASGVQIDIDSSTVVSPALITLAKKLGFDAHELALSGGEDYALLATFPQGT
                     IPVGFTVIGTCRQGHGVWVDGIPLPPKGHDHFRSP"
     gene            complement(5778..7196)
                     /locus_tag="PK710_06730"
     CDS             complement(5778..7196)
                     /locus_tag="PK710_06730"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT09444.1"
                     /translation="MKRDDGLEPNLEDLTTTKNIRLEDLVDRDSLKEVITSFVNLFGI
                     SVRLFSSEGDLLADAVEQQEVCKVINEYPDGRAACTATISTVKHTVPDPTQDTVHPCF
                     SGARYHIAAITYDGRYVGRIVLGPYLPAEVKELPGSLLTTVREAPPSRLQQLLPQMPR
                     ARVETVRNIAEHMRGILDLILFSGHKALLTSQMHLASVRESYRELQDKNQRLQDAYDK
                     LKELDRLKSNFIATISHELRTPLTSIIGYSEMMSEGIAGSLTDEQLEFVNTIRDKGEQ
                     LLTLIMSLLDLSKLESGSLALVKSDVDIRKSLDDVVSLLKPNATKAGVQLHVDVPAAL
                     PTIRGDANRIRQAVLNLAENAIKFTPSGGQVSLSAKLAQQVLDDAEDDIGFALIAPTR
                     SVIEIRVADTGIGIPENEREKIFTPFYQVDSSSTREYGGTGLGLSIVQRLIHAHDGKI
                     SVEPNHPKGSVFILKIPVDLDA"
     gene            complement(7193..7921)
                     /locus_tag="PK710_06735"
     CDS             complement(7193..7921)
                     /locus_tag="PK710_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HOT09445.1"
                     /translation="MQLDFVSRELTIKLVYYGPALSGKTTNLQAIHGFSDEQTRGRLM
                     TLETRDDRTLFFDLLPLRFRGEGDLSVRLKLFTVPGQVIHASTRRLVLQGTDGVAFIA
                     DSQLSETQNNARAFLDLKENLRFHGREMRELPLVIQYNKRDLPQTRSRDEIVQLAQKG
                     REPVFTAIATQGVGVLDTFVALLHLTWLSLDAAHDLSGKFRFNSEALLQEVITRMGAR
                     GTIDQFLNARLGGQFEARRKESGQ"
     gene            8187..10181
                     /locus_tag="PK710_06740"
     CDS             8187..10181
                     /locus_tag="PK710_06740"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT09446.1"
                     /translation="MFAIIITEKGGNERREVFDRTEVSVGRVQGNELMLPKGNVSKHH
                     ARLLYRDGRFIVTDLKSTNGTYVNGRKITQATIVREGDKIYVGDFILRLEVPQGAGGA
                     VVAAGGPSVTPSEDSPERISSSRSPSMRDVPPPSRPPVRRGAEGPVSHYPLENDPDEQ
                     SWSGDASPLDGPAPVPAHPAPLRVPAPPRVPSPPARVSPQASPSPHRPGSQADVTSPT
                     QAASAQPNRAPLPRSSARDVGFSSDSIQVPAQRIAAFTVIERVEQTPEIKAISDGKEV
                     SQATADRVERVLRETVSALRASGEMAPQIDPESVIQEAREELLGLGPLGPLLADEEVI
                     EIHVHRYAWVSVIRTTGPRRAEIPFASGSSLDRILRRLCASAGSPIASGEAMVERQVH
                     AGSTKLIAAFPPVSGTGPVATLLKCPPVTLSLEDLVRAGTLSRAMASFIGQAITGHVN
                     ILVTVAPGADGRSIIASLLSAVRPDEHVVVAHDAGCPLTLPRNGTSILTQPTREGASA
                     VRIAARLGSDSLFVTHCEGFVTSEVLDAVASGCDGVVAFMQAPSLRHAVARAAPEIAA
                     LRPGLPIESVREWLSASFDLAIEVVRLRDGRSRVARIAEPAGVEGNIIAMRDIFTFSV
                     ERTASGGTVEGSFHPTGVVPKVADNLQARGVPIDPNLFRR"
     gene            complement(10319..10822)
                     /gene="def"
                     /locus_tag="PK710_06745"
     CDS             complement(10319..10822)
                     /gene="def"
                     /locus_tag="PK710_06745"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOT09447.1"
                     /translation="MAILPIRVFPDPVLRERAKPVDQITPELLRLIDDMAETMYAAPG
                     VGLAANQVGVLHRVFIIDIASEDEPSDLHVFINPQIVHREGELTWNEGCLSFPGVSET
                     VERSAKVRVQALDRTGTPFELEAEGLLAVAIQHENEHLDGELMIDHLGPVRRRLVQRQ
                     LAKHRSR"
     gene            10821..11030
                     /locus_tag="PK710_06750"
     CDS             10821..11030
                     /locus_tag="PK710_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09448.1"
                     /translation="MMQNYSYVARRALASVGLRSGRTTAQKNRCTKATYKPNDASTVA
                     VTHSPTVFPRKPFVSDWAEKGGCVS"
     gene            complement(11047..12168)
                     /gene="ribD"
                     /locus_tag="PK710_06755"
     CDS             complement(11047..12168)
                     /gene="ribD"
                     /locus_tag="PK710_06755"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOT09449.1"
                     /translation="MPTEADIDLKMMTKALAEARRGRPSPNPHVGAVVARGHEVISVG
                     HHERAGTEHAEVVALRAAGEAAKGATLYVTLEPCNHTGKTPPCVDLILQAGIARVVIA
                     CRDPNPHVEGGGIEVLEAAGVQVDLGVKGIEARALIQPWVKQITTGLPYVSLKLAVSL
                     DGRIATRTGASKWVTGPEARAKVHEIRASHDAVMVGVGTVLADDPQLTVRDVEGPNPI
                     RIAVDSKLRIPLSANLIATAMDTPTWIVTAQDAPESIESTLVAQGVEVIRVARSSEGR
                     VDITAGLRAIAGLGIVRLLVEGGAELAGSLLNAGLVDELHVFIGPMLLGPRGRPGAVD
                     WAGPANLADAPTMVEPRWELCGRDAYVHGLLTATTSSRA"
     gene            complement(12186..12650)
                     /gene="nrdR"
                     /locus_tag="PK710_06760"
     CDS             complement(12186..12650)
                     /gene="nrdR"
                     /locus_tag="PK710_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOT09450.1"
                     /translation="MKCPHCGQLENRVIDSRLSTSGEVTRRRRECENCGRRYTTYERV
                     EDLLPSVVKRDGRREFFDRRKIVSGIRLACNKRPVSMSQIDEIAESIERELQESGERE
                     VSFTTIGEKVMNRLRKLDQVAYVRFASVYRQFRDLEDFSRELATLVTEPPSQ"
     gene            complement(12647..13096)
                     /gene="rpiB"
                     /locus_tag="PK710_06765"
     CDS             complement(12647..13096)
                     /gene="rpiB"
                     /locus_tag="PK710_06765"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOT09451.1"
                     /translation="MTPLAIASDHGGFALKEALKQALMQWGIAFEDLGPFDPSSCDYP
                     DYAHLMARVIGQGRFPRGVLICGTGVGMSMAANRHPHIRAAVVSDTYSARMAREHNDA
                     NVLCMGERVVGQGLACDILRVFLDTPSDSSDRHARRIAKLSPGEPVE"
     gene            complement(13144..>13508)
                     /locus_tag="PK710_06770"
     CDS             complement(13144..>13508)
                     /locus_tag="PK710_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HOT09452.1"
                     /translation="EQIEYINAHGTGTPQGDVQECKAIVDVFGHHASDQRLWVSSTKS
                     MTGHLLGAAGAAEAAFTALACARGLVPPTINIGEKDPQCAVDVVANEARERSVRHALS
                     NSFGFGGTNVSLVLSRVN"
BASE COUNT         2711 a         3764 c         4015 g         3018 t
ORIGIN      
        1 gtgtgaagtg tcgaatcacc ggattgtatc ttcgttcagg agtattcgaa gtattgccgg
       61 tcgatgacaa aattcggcgt atgattgcca cgtcggtcag ttcgaaagaa atcatgaaac
      121 aagcgcgtca ggatggtttg ttgacgctac gagaggccgc cattcgcaaa atggccaaag
      181 gggtgacgtc gttcgaggag gtattgcgcg tgacggtgga cggatgaaag cggagcgttc
      241 gatagaaaga tttgcccgaa agctgaggcg atattgggaa gcggggtatc cgattgtttg
      301 gctggtgacg tatgaagaac agcgggcaat cgaggtgttg cgtcggctcg cgccttcctg
      361 gccgattcag atttggtcat ggtcagggcg gcaggcagag gatgatcttg atgagatgtt
      421 ggcgaaggcc gcgagcagtg agtcaccggg actgaacgtg cttctcgacg ttcccacgcg
      481 gtgggcagag ggccggatga tccgcgcatt gaggaatttc gcggcttcgg ggcgaccggg
      541 acgtcatgtg atcatcgtat caccgtcgtt ggaactgccg gtggagttgg agaaagacgt
      601 atgtgtattg gatttccctt tgccggatga ggtggatttg agggcggcgg tgcgcgggga
      661 ggcgcaacga aaagggatag ggaattttga cgaagagcat ttggcgcgtt gtgcgggcgg
      721 catgacgtgg ttggaggcgc aacgggcttt tcgcatggcc ttgtccgtcc aagacccatc
      781 gcaatctgct cgtctggtgc tttcggagaa gcgtcaggtc atgcggcgaa gcgcagccct
      841 ggagcttgtg gagccggagg tggacctgca agacgttggg gggctcgagg tgttgaaggg
      901 ttggttgcgc acccgtgtcg tggcttgcgg accggaagcg cgtgcgtttg ggctaccgga
      961 gcccagggga atgatggttt gtggcgtgca ggggtgtggc aagtcgttgg tggccaaagc
     1021 gacggcatcg gtatttggtc ttcctctggt tcgtcttgat tttgcggccg tgtttgcctc
     1081 ctcttctccg gaacttgcgg tccgccaggc cacgcgcatg gccgaagcga ttgctcccgt
     1141 tgtgttgtgg gtggatgaga ttgaaaaagg attggggcag gggacgttgg agagcaaaaa
     1201 tgcccgggta ttcggcgatt ttttgatatg gatgcaggaa aagggggcgc cggtattcgt
     1261 ggcagcgacg gccaatgagg tggagcagct tccgcccgag ttggtgcggc ggggacgatt
     1321 cgacgaggta tttttcgtgg atttgccgag cgcccgggag cgagcggaca tccttgccat
     1381 ccacttgcgt cgaaggggca ggcatgcgga acggtatccg ttggaagagt tggtgaaacc
     1441 gtgggattac gtttccgggg ccgagataga acaagtggtc atcgccgcgc tttttcgcgc
     1501 ctttgccgcc aaacgggaat tggaggccga ggatattcgg cttgcgggcg gggaactcgt
     1561 gcctctagcc accatgtacg aagaacgggt tcaggccctg cgaacctggg ctacctccag
     1621 agcgcgaaag gcctcgaccg accgtcgtac cctcgagcta ttcgaagact aatccttcct
     1681 catgaccatg cctcgttcgt gatggtattc ggtcggcgtt gcgctacgtt ccaaagaaat
     1741 gaaccttggt ccgggtgaaa agacgatcct tctcatggca ggattcgtca ttgttgtcgc
     1801 tggcttacgc tttggtgagc ccttgttggt tcctctgttt ctcgccttgg tcatcgcgac
     1861 ggtttcttcg cccatcatcc gttggttgga aaagcgccgt gtgccaagcg cccttgccat
     1921 catcttggcc atcgtgctcg atctcgccgt tctcgcggcg ctgggtactc tcgttggatc
     1981 gtcccttagc gacttttccg aagccgctcc cagatatcag gcagctttga gcaacctcat
     2041 caaaaccaac gtcgcctggc ttgcggccag aggggttccg gcttcgagcg acttgcttgc
     2101 gccgcttcag gacttcggac gtctggtcga ccttcttggc ggcctggtca aaagcgttgc
     2161 caatgtggtg agcatgctgc tgttcgtgtt gttgctcgtc gtgttcatgc tcgtcgaatt
     2221 cggtcgctgg cagctcaaga ttggatatgc cttgggagac cgtcatgcgg atctgagtcg
     2281 cttcggcaac gccgcgcgcg aactgcaaaa atacctcggt gtcaagacgg cggccaatgc
     2341 cgtgacgggc ctattatgcg gcgtgtgggc cgccatcatg ggagtcgact tccccatcct
     2401 ttggggcctt atcgcgtttt tgctcaacta tatccccacc attggaacca tcatcgcagc
     2461 catacctccc atcgtgttgg cttggcttgc cttcggcccc ggcactgctt tcgtgaccgc
     2521 cgtgggcttc gtcgtcatca acgttacgct tggcaatgtc atcgaaccgc gaatcatggg
     2581 aagagccttg ggcttgtcac ctctggtcgt tcttatttcc atggtgtttt ggtggtggct
     2641 gtgggggccg gtgggcgcgc tgctttctgc cccattgacc atgatggtca aaatcatgct
     2701 cgcgaacacc accgacttgc gttggctggc tatcatgctc ggttccccct cgtgggtaga
     2761 agaaatgcaa gcgcaatggg atggggctag gcctgtcgag cacaaaagcg aattgtgcgt
     2821 tcgatcagaa aaacctgcgc atgaagtcct cctgggggtt cgagctaccg atccgccacg
     2881 cgacccctca gccactccct atgcgattcc acgctcccca gaatagcgta acctgttgaa
     2941 ataattatac ttaactcccg tcttccggca agtcccgatc ggttcggctc cgaagatatt
     3001 cgatggaagt tcgcttcctt cgcgttcgcg aaaagcgcct cacgttgccg cggtgttcca
     3061 acacgactat ggacgtgggt tcgttcccca atagacctgc gccgtgcccg tctcatcacc
     3121 tactcctgat gcccatgctc cgcctcgaga tcaacgtcca tggtggcgaa gagcactgcc
     3181 ttttgcgatc gcgctaggct tgctgtcttt gctgatatct cgtctggatc tcgccatgtt
     3241 cgcgcgacat ctgcggcagg tgaacaccgt tggctttctt gcgttctgtt tcgtattcag
     3301 catcacgttg ttggcatccg actcgttggc cacgtctttc gtgtattccc gcaccgtatg
     3361 tcccgtgcgt tttcatgaag tgttcttgat ccgtggcgca tcgtatttgc cgtccatgct
     3421 caaccaccat ctgggccaag cgtggctgac atggtatgtt tcgcgagttt ataaggctcc
     3481 tttgggaaga gtcgcggggg cgacactgct cgtgtacggc accaccttcg gatgtttgtt
     3541 attgcttggg ggcgtatcgc tcgcgttgga tgcatcctcg gcgcgatggc tcggaccgat
     3601 catgatagcc gcaagcgtgg gggccgtggt ttacctcgtc gtgattgcca gtaggccggc
     3661 gatcctggtg cgacgacaag tgctcgcgcc gttgttcgac gttggagtgg tcggtcacct
     3721 tctcgccttt gctcttcgag tgccgcatat cttggtgttg tttcttggca cctggctgtc
     3781 gtttcgtttt tttgacgtcg atattccacc ggctgtcgcg ctcgtgacca tgcctgtgct
     3841 gttggtcatt tccgctttgc cggtgacgcc gcaaggcgtg gggacgcgag attggttttc
     3901 gcttcacgcg ttttctcaat acgccatcgg tgacaaaatg gagcaggaag ccgcggttgc
     3961 ggccacgacg ttgtcttttg cggttgggat ctcgatattc caagctgccc ttgctcttta
     4021 cctgatgcat cgagcgttgc gtgtgcttgc gatgacgaag gataaggcaa gttgatgggg
     4081 cgtgttcaag ggcgcggttc gtgggtagca acccagttga gcccatcgtt ggcatacgac
     4141 gcggggtagg ctagctcgcc ttccgcaatg gacaggattt ccatcgatcg ggtgtcgatc
     4201 agcgcattcc aaggcacgcc tcggatcgtg gcgaccgtgc ccaaccgttt tgcgttgacg
     4261 tcgatgagta cggaaaaggg cgtgttcatg gtatctaccc aatcgtcgac atccgccagc
     4321 gaaagagggc tgccccgacg ttttccctcc acgatggttt gcaccacacg gataccgttt
     4381 gcgcggaaag agtcgttgag tttcacgacc gcgcgtgttt ccgcctggca atgggggcac
     4441 cacgcaacga caaccatgag aaacattagc ttatggtgct gttgcgatgg atcgaaatag
     4501 tccgccatgc ttaccacctg cagtccgtcg tcctttcgag agtccggata cccctgaaaa
     4561 ctaaaattag gcaggcgttg ccccggctta tttccgtccc tcggtcgagc cccccaatcg
     4621 tccgatggat aggggattcc ctccggattt cggtcggtgc ccacgacatc cacatcgttg
     4681 aatggtgtgg gtgagtcctc gctcgagcag gcggtcccca cgccgatagc gaacaagggg
     4741 atggaaaaaa accaaatggg atgcatcgat ctttgccgtt catcaggcac cccgttgccg
     4801 tcgtcaagtt ggtaaggaca ctggccgggt ggtgctcagg ggcttcgaaa atgatcgtgc
     4861 ccttttggcg gcaaggggat gccgtcgacc cacacgccgt gaccttggcg acacgtgccg
     4921 atcacggtga agccaactgg tatggttcct tggggaaagg tggccagcag ggcataatcc
     4981 tcaccgccgg ataaggcgag ttcgtgagca tcgaatccca gttttttggc gagcgtgatc
     5041 agggcagggg agacgacggt agaggagtcg atatcgattt gaacgccact tgccatggca
     5101 aggtggagcg catcttgcgc gagtccatcg gagatatcga tgagcgaaga ggcatgcatg
     5161 catcgcaatc cttcttcgat acgagcacgg ggccgtcgcc acgcatgcaa agcgatggtt
     5221 tcgatttcat cggcagcggt ccacgaaggt tttttcaaga gcattcgcag gcccagcgcg
     5281 gcgaatccga cggggccgca caaggctagc tggtcgccgg gacgagcccc atggcgacga
     5341 ggagccaggg tggttcgacc caaccacgtg gtggagacgg tgacgatctg tgaggccgaa
     5401 aggtttcccc cgatgaccga tgatccgagt tgttgagcgg cgagtttttg cccattggcc
     5461 aaggcgtata atgcatcgtc gggcaggttt cgtggaaccg atacgctgca caaggcgcca
     5521 atcggtgttg cggccatggc ggcgagatcg cttgccgcgg ccatcatcga acggaatccc
     5581 acatcctccc agctagcgat atcttggcga aaatgaacgt tttcgataca ggcgtcgatg
     5641 gtccagacga ttttttcgcc cacaggaaca tcgatttccg cggcgtcgtc gccaatgccg
     5701 acggacactc cctcgggagc aggtccgaac aagcgacgaa gatgctcaat gcgttcgaac
     5761 tcaccggcca tgggtactca agcatcgagg tcgacgggaa ttttcaagat gaacacgctg
     5821 cctttggggt ggttgggttc gacggatatt ttcccatcat gagcgtggat gaggcgttgg
     5881 acgatggaaa gtcccaaccc ggtaccgcca tattcgcgtg tggacgagga gtcgacttgg
     5941 tagaaagggg tgaagatttt ttcgcgttcg ttttcgggaa tgccgatgcc agtatccgcg
     6001 acacggattt cgatgaccga acgggtggga gcaatcaaag cgaatccaat atcgtcttcc
     6061 gcatcatcga ggacttgttg ggcgagtttg gcggatagtg agacctgacc gccggaaggt
     6121 gtgaatttta ttgcgttttc cgcaaggttg agcacggctt gacggattcg attggcgtcg
     6181 ccgcggatgg taggcaaggc ggcgggaaca tcgacatgga gctgcacccc cgctttggtg
     6241 gcgttgggct tgagcaggga gacgacatca tccaaggatt ttcgaatgtc cacatcggat
     6301 ttgacgaggg ccaagcttcc gctttcgagt ttggataggt cgagcaaact catgatgagc
     6361 gtcagcagtt gctcgccctt gtcgcggatg gtgttgacga actcgagttg ctcgtcggtg
     6421 aggcttcctg caattccctc actcatcatt tcgctatagc cgatgatgga ggtcaaggga
     6481 gtgcgcagtt cgtggctaat ggtagcgatg aagttggact tgaggcggtc gagttccttg
     6541 agtttatcgt aggcgtcttg cagacgttgg tttttgtctt ggagttctcg atagctttcc
     6601 cgcacgctgg ccaggtgcat ttgcgaggtg agcagggctt tgtgcccgct gaagaggatc
     6661 aagtcgagga tcccccgcat gtgttccgcg atattgcgaa cggtttctac tcgtgctctg
     6721 ggcatttggg gaagcagttg ttgcagtcgg ctcggtggcg cttctcgaac cgtggtcaac
     6781 aatgagcctg gcagttcttt gacctcggct ggaagatagg ggccgaggac gattcgcccg
     6841 acataacgtc cgtcatacgt gatggccgcg atatggtagc gagcgccgga gaagcaaggg
     6901 tgaacggtgt cctgggtggg gtcgggaacc gtgtgtttga cggtagaaat ggtggccgta
     6961 caagctgccc tgccgtcggg atactcgttg atgaccttgc agacctcttg ttgctcgacc
     7021 gcatcggcga gcaagtcgcc ttcactcgaa aacagccgta cggagattcc gaaaagattg
     7081 acgaaagaag tgatgacttc tttgagagag tcgcggtcga ccagatcctc gagccgaata
     7141 tttttcgtgg tcgtaagatc ttcgagattc ggttccaaac cgtcgtcgcg tttcattgcc
     7201 cggactcctt gcgtcgggct tcgaattgtc ctccgagccg agcattcaag aactgatcga
     7261 tggtgccccg tgcgcccata cgagtgatga cctcctgtag taacgcctcg gaattgaatc
     7321 gaaatttccc ggacaggtca tgggcggcat ccagactcag ccacgtcaga tgcagcaatg
     7381 cgacgaacgt atccagcaca ccgacacctt gggtagcgat cgccgtaaac acgggctctc
     7441 tgcctttttg cgcgagttgc acgatctcat cacgggatcg tgtctggggt aagtcgcgtt
     7501 tgttgtattg gatgacgagg ggcaattcgc gcatttcgcg gccgtggaac cggaggtttt
     7561 ccttcaagtc gaggaaggct cgagcattgt tttgcgtctc ggacagttgt gaatccgcga
     7621 tgaacgcaac accatcggtt ccttgcaata cgagccgacg tgtcgaggcg tgaatgacct
     7681 gccctgggac ggtaaatagt tttaatcgta cggataaatc gccttcccct cgaaaacgaa
     7741 gcggcaataa gtcaaaaaac aaggttcgat cgtcgcgcgt ctccaacgtc atcaggcgac
     7801 cccgggtttg ctcgtcggaa aaaccatgga tcgcctgaag attcgtggtt tttccgctca
     7861 aagcagggcc gtaatagacg agcttgatcg tcaattctcg agaaacgaag tccagttgca
     7921 cgcttggcac cttcgcggtg gggttaccga acgggatgaa accagaagat gcacgatgct
     7981 actcgaaatc gagcgtttag gggacaaccc aatctctcat tcacggaatt gagcgaataa
     8041 tcagggcgaa gatatataag gcaggtccct cacgcgcttg cgggaggcat tcccgaacgc
     8101 ccaggcattg attcgattga catcaccttg cgctcgggtt gcaatcggca ctagtggtgt
     8161 gagtaatctc acgcccaaag gctggaatgt tcgcgattat cataacggaa aaaggcggca
     8221 acgaacggcg cgaggtgttc gatcggacgg aggtcagcgt cgggcgcgtg caaggcaatg
     8281 agttgatgct tccgaaaggc aatgtctcaa agcatcacgc gcgattgctg taccgtgatg
     8341 gccgattcat cgtcacggac ttgaaaagca ccaacggcac gtacgtaaac ggtcgcaaaa
     8401 tcacgcaggc cacgatcgtg cgggaaggcg acaaaatcta tgtaggcgat ttcatcttgc
     8461 ggctcgaagt gccgcaaggc gccggagggg ccgtcgtcgc cgccggtgga cccagtgtca
     8521 ctccctccga ggactctcca gaacgtatct cttcgtctcg tagcccaagc atgcgcgatg
     8581 ttcctccccc tagtcggccg cctgtacgac gaggtgccga aggacctgtc agccattacc
     8641 cgttggaaaa cgatcccgac gaacagtctt ggagtggcga cgcatcgccg ctggatgggc
     8701 ccgcccccgt gccggctcat cccgcgccct tgcgcgttcc tgctcccccc cgggttccct
     8761 caccgcccgc tcgcgtatcg ccacaagcgt cgccatcacc acaccgacca ggcagccagg
     8821 ccgatgtcac ctcgcccacg caagccgcat cggcacaacc caatcgcgcc cccctgccac
     8881 gctcgagcgc tcgcgacgtg gggttcagct ccgactcgat ccaggtgccc gcacagcgca
     8941 ttgccgcgtt cacggtcatc gaacgggtcg agcaaacgcc ggaaatcaaa gcgatttccg
     9001 atggaaaaga agtgtcccag gcgacggcgg atcgagtcga acgcgtcttg cgcgaaacgg
     9061 tctcggccct tcgtgccagt ggcgagatgg ctcctcaaat cgatccggaa agcgtgatcc
     9121 aagaagctcg cgaagaactc cttggattgg gccctctcgg acccttgctc gcggacgagg
     9181 aagtgatcga aattcatgtg catcgatatg cctgggtgtc ggtcattcgc acgacaggtc
     9241 cacgacgtgc cgagattcct tttgcttccg gaagttccct cgaccgtatt cttcgacgac
     9301 tgtgtgctag cgcgggctcg cccatcgcta gcggcgaagc catggtggaa cgacaagtgc
     9361 atgccggatc cacgaaactc atcgctgctt tccctcccgt gtccggcacg ggaccggtcg
     9421 ccacattgct gaaatgcccg ccggtaacct tgtctctcga agatctggtg cgggcaggaa
     9481 ccttgtcccg ggccatggcc tctttcatag gacaagccat cacgggacac gtcaatatct
     9541 tggtgaccgt ggccccaggc gccgatggta gaagcatcat tgcaagtttg ctcagcgccg
     9601 ttcgacccga cgaacacgtt gtggtggctc atgatgccgg gtgcccactg acgctgcccc
     9661 gcaatggaac cagtattctc acccaaccca cacgcgaagg agcttccgcc gtgcgcatcg
     9721 ccgcacgcct cggttccgat agcctcttcg tcacacattg tgaaggcttc gtgacctccg
     9781 aagttctcga tgccgtggca agcggttgtg atggcgtcgt cgctttcatg caagctcctt
     9841 cgctgcgtca tgctgtcgcg cgagccgccc ccgaaatcgc tgccttacgc ccaggtttgc
     9901 ccatcgaatc cgtgcgagaa tggctaagcg cttcgttcga tctcgcgatc gaagtggttc
     9961 gcttgcgaga tggtagatcg cgcgtcgcgc gcatcgcaga acccgcggga gtagaaggca
    10021 atatcatcgc catgcgcgat atcttcacct tctccgtcga acgaaccgct tctggcggca
    10081 ccgtggaagg cagtttccat ccaacaggtg tcgtccctaa ggtcgccgat aaccttcagg
    10141 cccgtggcgt acccatcgac ccgaacttgt tccgtcgatg accctcataa cgaaccatca
    10201 cctcatgcga tgacccgaat agaaggtccg tcgcaaacac atatatcctt ccctcacacc
    10261 cgcaccttaa ggtcgtgtgg atccgcgagc cagcgccacg cgctcatgct ctcattcact
    10321 aacgactacg atgcttggcg agttgtcgtt gcaccaaacg tcggcgcacg ggccccaaat
    10381 ggtcaatcat caactcaccg tcgagatgct cattttcatg ctgaatcgca accgccagga
    10441 gcccttcggc ctcgagttcg aacggtgtac ccgtgcggtc caacgcctga acccgaactt
    10501 tcgcggaccg ctccactgtc tcgctaacgc caggaaatga aagacaaccc tcgttccacg
    10561 tcagctcccc ttctcgatga acgatctggg gattgatgaa gacatgcaaa tcgctgggct
    10621 cgtcttcgct cgcgatgtcg atgatgaaca cgcgatgaag gacgcctacc tggttggcag
    10681 cgagacctac acccggggcc gcatacatcg tctcagccat atcatcgatc aaacgaagca
    10741 attccggagt gatttggtcc acgggcttgg cgcgctcgcg aaggaccgga tcgggaaata
    10801 cgcgaatggg aagaatagcc atgatgcaaa actattcgta cgtcgccagg cgcgcgttgg
    10861 caagcgtagg tctacgttcc ggtcgaacga cagcgcagaa gaatcgatgc accaaggcaa
    10921 cctacaaacc caacgatgca tcaaccgtgg ccgttaccca ttctcccacg gtttttcctc
    10981 gcaaaccgtt cgtcagcgat tgggccgaaa agggaggctg cgtatcgtga atcggcgacg
    11041 cgaccgtcag gcgcgcgacg atgtggtggc ggtaagcagg ccatgcacgt aggcatctcg
    11101 tccgcaaagc tcccaccgtg gttccaccat ggtcggtgcg tcggcaaggt tggccggccc
    11161 agcccaatcg accgcgccag gacgccctcg aggccccaac aacatgggcc cgatgaagac
    11221 gtgcagctca tcgacaagtc ccgcgttgag caggcttccg gcgagttcgg cgccgccctc
    11281 gaccagcagc cggacgattc caagaccggc tatggctcgc aatcccgcgg tgatatccac
    11341 tcgtccctcg ctcgaacgtg ccacgcgaat gacttcgact ccctgggcta ccaaggtgct
    11401 ttcaatggat tctggtgcat cctgcgccgt aacaatccaa gtgggcgtat ccatagccgt
    11461 agcgatgagg ttcgcggaaa gaggaatacg aagcttcgaa tcgaccgcta tccgaatcgg
    11521 gttaggacct tcgacgtcac gcacggtcag ttgagggtcg tcggccaata ccgtaccgac
    11581 accgaccatg acggcgtcat gggaagcacg aatttcgtgg actttggccc gtgcttccgg
    11641 tcccgtcacc catttgctcg cgcccgttcg cgtggcgatt cgaccgtcga gagatacagc
    11701 aagtttgaga ctgacgtagg gtagacccgt ggtgatttgt ttgacccacg gttggatcaa
    11761 cgcgcgagct tcgatgcctt tgacaccaag atcgacttgc acgccagccg cttcgagcac
    11821 ttcgatacca ccgccctcga catgaggatt ggggtctcgg caggcgatta ccacccgcgc
    11881 aatgcccgct tgcaagataa ggtcaacaca tggaggggtt ttgcccgtgt gattgcacgg
    11941 ctcgagagtg acatacaagg tcgcgccctt cgctgcttcc ccagcagcgc gcaaagccac
    12001 gacttcggca tgttccgttc ccgcgcgttc atgatgcccc acagagatga cttcgtgacc
    12061 acgagccact accgcgccta catgaggatt gggcgaagga cgtccacgcc tggcttctgc
    12121 aagcgctttc gtcatcattt tgagatcgat gtcggcttcc gtcggcattc gtaacctcgc
    12181 aagctttact gcgatggggg ctcggtgaca agcgtcgcca actcacgaga gaagtcttcg
    12241 agatcccgaa actgacgata aacgcttgca aatcgcacgt atgccacttg atcgagcttt
    12301 cgcaagcggt tcatgacctt ctctccaatg gtcgtgaagg acacttctcg ctcaccggat
    12361 tcttgcaatt cgcgttcgat actctccgcg atttcgtcga tttgactcat cgagacagga
    12421 cgtttgttgc aggccaaacg aataccgctg acgattttcc gacgatcgaa gaattcgcga
    12481 cggccatcgc gtttgaccac ggacggaagc aggtcttcga cacgttcgta ggtggtatag
    12541 cgacgtccgc agttttcaca ttcacgacga cgacgtgtga cctctccgct cgtgctgagt
    12601 ctcgagtcga tgacccggtt ttccaactgg ccgcaatggg ggcatttcat tctacaggct
    12661 ccccaggcga caatttggcg attcgtcgtg cgtgccgatc gctcgaatcc gaaggggtat
    12721 caagaaaaac tcgaaggatg tcgcaggcaa gtccctgacc gacgacacgt tctcccatgc
    12781 acaatacatt ggcatcgtta tgctctcgcg ccatgcgagc ggagtaggtg tcggatacga
    12841 ccgcagcgcg aatatgcgga tgtcgattgg ccgccatcga catccccacc cccgtgccgc
    12901 agatgagcac accacgggga aaacgccctt gcccaatcac tcgcgccatg aggtgcgcgt
    12961 aatccgggta gtcacaggac gaagggtcga agggtccaag gtcctcgaaa gcgatgcccc
    13021 attgcatgag tgcttgcttg agggcttctt tcagagcgaa tccaccgtga tcggaagcaa
    13081 tggcgagcgg ggtcatgcgt aggttctagc gttgatggta gggaaaaaaa agatggatga
    13141 gattcagttc acgcgcgaca ggaccagcga gacgttcgtg ccgccaaatc cgaaagaatt
    13201 ggacaaagca tggcgcacgg agcgctccct tgcctcattg gctacgacat ccacggcgca
    13261 ttgggggtct ttttcgccga tgttgatggt ggggggaacg agccctcggg cgcacgccag
    13321 cgccgtaaat gccgcctccg cagctccggc agccccaagc aaatgccccg tcatcgattt
    13381 ggtcgacgat acccataaac gttgatcgga agcgtggtgg ccgaatacat ccacgatagc
    13441 cttgcattcc tgcacgtcgc cctggggagt gcccgtacca tgcgcattga tatactcgat
    13501 ttgctcgg
//
LOCUS       DAOSOR010000087        13488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4591_length_13488_cov_4.237996, whole genome shotgun sequence.
ACCESSION   DAOSOR010000087 DAOSOR010000000
VERSION     DAOSOR010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 13488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13488
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4591_length_13488_cov_4.237996"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..1607
                     /gene="malQ"
                     /locus_tag="PK710_06775"
     CDS             111..1607
                     /gene="malQ"
                     /locus_tag="PK710_06775"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOT09453.1"
                     /translation="MDASSTRSAGILLHPTSLPGPHGIGSLGSYARGFIDFLAAAGQS
                     YWQILPLGPTGYGNSPYSALSTFAGNPWLIDLQTLVTEGLLHGSELAAAPLCTDRVDY
                     GAVGPFKTEKLHLAAERFATVASAERREKFEAFEQQNAWWLDNYALFEAVKQANEGKS
                     IRDWDTELRCREPAAIARASQQLATEIRHQRVMQFLFFEQWAALRSYAHGRGVRIIGD
                     MPIFVSFDSDVVWAHPELFKVDANLTPSVVAGVPPDYFSATGQLWGNPLYDWKTHEKQ
                     GFNWWVDRVRMALLLADVVRLDHFRGFEAAWEVPSHHADACHGTWVKGPGNAFFDALR
                     SELGSLPFIAEDLGLITKEVYALRDHYGLPGMRVLQFGFGTGEDRYNAPHRVERNSVV
                     YTGTHDNDTTVGWFRTLSAKDRAFVQSYLNTSGATIHWAMIRAAYGTVADTVIIPIQD
                     VLGLGSEARMNMPGQMGGWWAYRLTEQPHAAVAEKLRELTELYERCPS"
     gene            complement(1602..3842)
                     /locus_tag="PK710_06780"
     CDS             complement(1602..3842)
                     /locus_tag="PK710_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09454.1"
                     /translation="MKKTTPLGLFVTGLSLTLSVCAHATEWYQGSPSFRNHTNHPTTA
                     ASTYLQKHSSQLRLESVQLETKRVLPLRSLHTVRFAQHHHNLPVIGKYAVVRVGPEGS
                     VRVVALDVARGLRVDPQPTLTANDAIAEVAAALHVPLPPPLAHASLVVLAEEPSDEML
                     AWQVDTVQPYGMHRFFVDAHRGGVVAHRSMARHALGRVFDVNPIVTPNLVERELIGLS
                     PAVPTSLTGFEEALRVHAYVAGDIMYGGLQTNQTPPSEGEDFLFTPNFDPWGGNDPFG
                     EVMGYYHAARIRGFFENNLGVDMSGSEFSLAVITGYAPESSPEYIQNAFYSPWPPNYP
                     GIPGNPRNIICLGRGYSENLAHDSDVLLHEFTHYMSHNALDYTSSSLFDAYGYVSMPD
                     AINEGTADYFSSSLNNDPQVGEYALGAWARNLDEPAGRCPENMIGESHEDGRIIGNLT
                     WTLRNMHGADKADQLVWGAMSLLGSRATFGDFGRGLLQSAADLNFDDQQIQQIENELK
                     DRGLDDCDRALEINGKPRVSYMLGLGELGSMMGYSCADMKASGFIMSSLFQYQYTPHP
                     TDSSLTLDVRFSNAHGGSHDMNWRIVVQRNRMAVVHSNLLVSPDYMLNPITDTHAQLV
                     IDADSTPPFDPSATYYAAILSENCWGASLHVEANPPETRPADAGADVAPEAEAKDAGW
                     DIDLVFDTAAPPEPSMTPELYPAGGCSCHTSPAAPSARWWWLIATSVLCVARRRPAPS
                     RIRPRS"
     gene            4122..5495
                     /locus_tag="PK710_06785"
     CDS             4122..5495
                     /locus_tag="PK710_06785"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT09455.1"
                     /translation="MVDDIKRTLRDSKAARWTALLIVSFSMLCGYYVADIMSPLKPMI
                     EQQLGWSSSEYGFFTGAYAWFNVFLAMLIFGGVILDKKGPRFAGVLGCVLMVVGCGAK
                     YFAISTDSLRDVMMLGTKAQVLIASVGFAIFGVGIEICGITATKIIARWFKGYEIALA
                     MGLQVSTARIGTALALGLGGPIAAALHSVAAPVLVGLLMLAVGLIAFFFYVVMDRKLD
                     ASETAAEAEPEEPFRLADIGQILRIRGFWYIAMLCALFYSAVFPFLKYAPDLMVQKFD
                     VRDDLAGVIPSILPFATIPLTVIFGGFYDRKGKGATIMALGSLLLVVVHLTFTIPMLS
                     HWVIAVVATVVLGFGFSLVPSAMWPSVPKFIPQRQLGTAYALIFYMQNLVALMGVPYL
                     IGWVLDRFCVIGTRDVEGVSSPAYDYTWPMAVFSALGVLAVVFALLLKAEDKKMGYGL
                     ELPCHNK"
     gene            complement(5523..6764)
                     /locus_tag="PK710_06790"
     CDS             complement(5523..6764)
                     /locus_tag="PK710_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09456.1"
                     /translation="MQLHHTPMALFFVLFAGCSQILGFDDLGFDDTAYPNDAAGTTDR
                     DVQDSSAQDSGVQDTSVQDNIVDTSSEESGLADSNETGMEDVGKEEGNSPECTNVGPA
                     YIHGDLWGFWHNIHVACDAQNRWLWLCQKRLGQGKCSIEAQRFQDCWTASGDFPPTTW
                     GTDATPHSPSPNVCQPNQYSEKNVASERPGNPVPCDTKTFDYDSLRQKDPFYGADWMP
                     YPINTRHLTLKVFAQGQDPLENEGQSDAMVVLSTDPSNKSAYTYEISNHSYPTRQRSG
                     GCLPPIKDDVEKPFPSQNFGAFFWLEVPTDEPVTLMASWIGPVDGTTIIAPVCSPGSS
                     IYSFPASFGQHGEDGKPWFISSPCWDIIKDVQLEPGRHYLWNINGLQLMPDCSGPPDS
                     LLQLVPSSSRASFKDGTCSSM"
     gene            complement(6848..9964)
                     /locus_tag="PK710_06795"
     CDS             complement(6848..9964)
                     /locus_tag="PK710_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOT09457.1"
                     /translation="MSALATSPHVAIRASAGSGKTYQLTNRYLGLLIRGAAPETILAT
                     TFTRKAAGEILGRVLTRLAKASLNSSLSVELGKQIGVPDLTTARVGQVLRSVTGALHR
                     LRVSTIDSFFSSIARSFSLELGFPAQWQIVSTSVADAMQNRAIEASLRGDVQDMVALV
                     RLLSKGQYVRPVVATVSEIVDSLHGTFLDAPAFAWQALTLDSPSDPTKIESILRQLEE
                     CQTRMTLPGSISKALAQSIEHVRAKRFREFVHAGLGRKVAEGQPTYRKANIPEELKDL
                     LQQLLEQVRTIFLEALIGRGRAIQRFLTDYDEHLRVLKQTQASYRFGDVARALATASL
                     LGRLDEVYFRLDCGIHHLLLDEFQDTSLSQWQVLRPLAQEVAAHASMQRSVLLVGDVK
                     QAIYGWRGGVADIFDSLDQDLPGLTWVPLHTSYRSSQPIIDTVNDVFEGLGDNQGFRE
                     EQAAARAWQAGFQRHTTAKTDLPGFVRLQVAPFDEQASAPVRQAADLVQQWVKRCPGV
                     TIAVLTRTNDAVRKVMAVLREKGIPASEEGGNPITDSAAVGLMLSLLRLADHPGDSIA
                     RHHLANSVLGEALGLRDDPGSAVAVSQKIRQQIQQDGFETCLRRWSLVVAPHCDAREA
                     RRLTQLLRLANQLDQRTFLRIDEFIEFASTERVEDPATSHVRVMTIHKAKGLQFDIVI
                     LPDLDKQLVGPVPAVLLDSVSAISPATRVVPYVNEEEQLLLPELGQLVKRWRAGQVCE
                     SSSLLYVALTRAVHALFMLIEPTGSASIPATFAGVIRHALAPGKAASAGACLFEHGDE
                     TWFQTLTFPPPEALPHEPAIAGITMLSSPARTKGLPKTSPSSLEGGKLFVPSDLLYLD
                     TSSLRMGSIIHALFEQITWLEEQQPRDEILHRKAIQAGANESNVRALVRQFQDMIAMP
                     QVHAVLSKQTYQLPEISVELHRELPFALHDDNSLLSGVIDRLVVRKHGDRVMDAEVLD
                     YKTDTVDEKSLADKVERYRPQLEAYRRAVARLLGVETDKIKTQVVFVRPGWVIDLPYP
                     SRLTEPCS"
     gene            complement(9961..12789)
                     /locus_tag="PK710_06800"
     CDS             complement(9961..12789)
                     /locus_tag="PK710_06800"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOT09458.1"
                     /translation="MPPRSHTAQPVQLDLGLSARQSEIQRVFLGWDRPLLPLSVEYFL
                     AQDVGDPIDLRSFLVVTPASRAGARLLEALVLHSGPKRALLPPRVITMGALSDSLIQP
                     CQPTAAPLTRSIVWAEALRSASPSLLETLVPTPPAPNDWPAWLQLGRMFDDLDAELAS
                     AALRIGDVPARAAHLEGFRDGDRWQAIAQIREGVHEHFQRLGIDDATARHLDAIATGN
                     VELAPPATTIVLVGCPELTVFTRKILDATRARVLALIHAPASLADRFDQFGAVLPSAW
                     EQGPLGEVTVNIVDDPAQQAACVAATLAELSPHKGLQDVVIGVPDVDVIPLLSETLEP
                     LGVPTRNAAGTPMLRRSVVRLLLAIANHLERQSFSTLGVLARIPDLSLLVESEEQDKD
                     WRVLLDIYQSKHIACNLTDHFVGSSDIAQSLVRRLNKLHACLADLLGSERPLGRWAPA
                     IASFLIRLFGEKPLDPTDAASKQTIAILVAFQDVLRTMAELPDSVVEKVSAATALRVV
                     VDSVAEIAIPEPAEREAIELLGWLELPHDDTAAVIVTGMNDGMVPGCRNADPFLPDGL
                     RHHLGLADNRLRFARDAFLLSALLASRQSVTLMAGRRSLAGDPLAPSRLALCGSTERI
                     AEHVLAFYEGSSEVLPVASLIQPGCQGPCLPVVRPKPLSHPIEAMTVTAFGDYLSCPY
                     RFYLRHVLGLRAAKDDAKELDGRGFGNLAHEVLKDFGHSEVATSTEVPLIREYLDVRL
                     DEWARSWFGDQAFSSVWVQVEQLRARLHAFARWQAQWAASGKRILHAEYEIDAGKIAL
                     NVDDTPMYLVGRIDRIDRDESTGTLYVFDYKVSQRPRDPVKDHLTKGEWKNLQLPLYH
                     RMVQAMARGKIELGYLSLSAAHEVQEALAGWSEQQLASAMQVAEHVVRSVRKQVFWPP
                     TDRPFFDDFDVICQSQRLGGAVLQGGTS"
     gene            13044..>13488
                     /locus_tag="PK710_06805"
     CDS             13044..>13488
                     /locus_tag="PK710_06805"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOT09459.1"
                     /translation="MSMRKYFHALGLLLLIALMAKPSQAASVCIDPGHGGSDPGAQGC
                     GLSEAKINLQVSLKLRDLLKASGVTVYMTRETDVFVGLSARASYANSKGVDRFASVHS
                     NAGGGTGIETFCMEGSSSTSLGYKMASAIQTQMLKVWPLPDRGVKK"
BASE COUNT         2905 a         3736 c         3959 g         2888 t
ORIGIN      
        1 gccgatttcg aaaacgttac tcagttttga gtggcgcaag agagcaaatg ccgataatac
       61 gaaatgacta tcttgggaat actccgtttc catgccatag gtcccccgtc atggatgcat
      121 catcgactcg aagcgctggc attttgcttc accccacctc gctgcctggc ccccacggca
      181 ttggttccct gggctcctac gctcgtggtt tcatcgactt tctcgccgct gcaggccagt
      241 cctattggca aattcttccg ttgggaccga cagggtacgg caattcaccc tactcggcgc
      301 tcagtacctt cgcgggcaat ccgtggctca tcgatttgca gaccctggtg accgaggggc
      361 tcctgcacgg ctcggagttg gctgcagcgc ccctttgcac ggatcgagtg gactatggcg
      421 cggtcggccc gttcaaaacc gagaaacttc accttgcggc agagcgtttc gccacggtgg
      481 cttcggcaga aagacgggaa aaattcgagg cttttgaaca gcaaaacgcc tggtggttgg
      541 acaattatgc tttgttcgaa gccgtaaaac aagccaacga aggcaagtcg attcgggact
      601 gggacaccga gctgcgttgc cgcgaacccg cggccattgc ccgggcgagt cagcagctcg
      661 ccacggaaat acgtcatcag cgggtgatgc agtttctatt tttcgagcaa tgggcggcgc
      721 tgcgttccta cgcccatggc cgtggggtgc gaatcatcgg ggatatgccc atcttcgtgt
      781 ctttcgatag cgatgtggta tgggcgcatc ccgagctttt caaggtggat gcaaatttga
      841 ccccatcggt ggtggccggg gttccgccag attatttctc agccacggga cagctttggg
      901 gcaatccgct gtatgactgg aaaacgcatg aaaaacaagg gtttaattgg tgggtggacc
      961 gggttcggat ggcgcttttg ctcgcggatg tcgttcggct ggaccatttt cgaggcttcg
     1021 aagccgcgtg ggaagtcccc tcccatcacg ccgatgcgtg tcacggaacc tgggtcaaag
     1081 ggcctggaaa cgccttcttt gacgcccttc gctccgaact gggaagcctt ccgttcatcg
     1141 cggaagactt gggtctgatc acaaaagaag tctatgcgct gcgagaccac tacggtttac
     1201 ccggcatgcg cgtccttcaa ttcggattcg gcacggggga agatcgctac aacgcccccc
     1261 atcgcgtcga gcgcaactcc gtcgtatata ccgggaccca tgacaatgat acgacggtgg
     1321 gatggttcag aacgctgagc gccaaggata gagccttcgt gcaatcctac ttgaatacgt
     1381 cgggcgctac cattcattgg gcgatgattc gcgcggcata cggtacggtg gccgatacgg
     1441 ttatcattcc gatacaagac gtgctgggct tgggcagtga agcgcgtatg aacatgccag
     1501 ggcaaatggg cggctggtgg gcgtatcgtt tgaccgagca gccccacgcg gcggtagcag
     1561 aaaaacttcg tgaactcacg gagttatatg agcgttgccc gtcatgaccg cggtctgatg
     1621 cgcgatgggg ctggacgtcg tctagccacg caaagcacac tggtcgcgat gagccaccac
     1681 caacgggcag acggcgcggc aggcgacgtg tggcaagagc agccccccgc ggggtacaac
     1741 tcgggagtca tcgaaggttc agggggtgct gccgtatcga aaaccagatc aatatcccat
     1801 cccgcgtcct tggcttccgc ttccggcgcc acgtcggcac cagcgtcggc cggccgggtt
     1861 tctggtggat tggcctcgac atgcagggaa gcgccccaac agttttcgct caaaatggcg
     1921 gcatagtagg tggccgaagg atcgaacggc ggcgtactgt ccgcgtcgat gacgagttgc
     1981 gcatgggtgt cggtgatggg attgagcata tagtccggtg aaaccagcag gttcgagtgg
     2041 acgacggcca tgcgattgcg ctgcaccacg atgcgccaat tcatgtcatg ggagccacca
     2101 tgggcattgc tgaatctgac gtcgagcgtg agcgatgaat ccgtggggtg aggcgtatat
     2161 tggtattgga acagagatga catgataaag ccggaagctt tcatgtcggc acaggaataa
     2221 cccatcatcg agcccaattc cccgagcccc aacatatagg aaacgcgcgg ttttccattg
     2281 atttccaggg ctcgatcgca atcgtcgagc cccctatcct tcaactcatt ttcgatctgt
     2341 tggatttgtt ggtcgtcgaa attcaagtcc gcggcggatt ggagcagccc tcgaccgaaa
     2401 tcaccaaagg tcgccctgga acccagcaaa gacatggctc cccaaacgag ctggtccgcc
     2461 ttgtcggcac catgcatgtt ccgtaaggtc caggtcagat tcccaatgat tctgccatct
     2521 tcgtgagact cgccgatcat attttccgga cagcggcccg ccggctcgtc gaggttccgc
     2581 gcccacgctc ccaaggcata ttcgcctact tgcgggtcgt tgttgaggga agacgaaaaa
     2641 taatccgccg tgccctcgtt gatcgcatcg ggcatcgaca catatccgta agcatcgaac
     2701 agggacgaac tggtgtaatc cagcgcgtta tggctcatat aatgcgtgaa ttcatgcagg
     2761 agcacgtcgg aatcgtgagc gaggttttcg gaatagccgc gcccaaggca gatgatattg
     2821 cgagggttgc ccggaatgcc gggatagttg gggggccaag gggaataaaa cgcgttctgt
     2881 atgtactcag ggctggactc cggcgcgtag cccgtgatga ctgcgaggct aaactcgctg
     2941 ccgctcatgt ccactcccag gttgttttca aaaaaaccgc gtatgcgcgc cgcgtggtaa
     3001 tagcccatca cttccccgaa cggatcgttg ccgccccagg gatcgaagtt gggcgtaaac
     3061 aagaaatcct ccccttcgct gggaggcgtt tggttcgttt gtagcccgcc gtacatgata
     3121 tcgccggcca cgtaagcgtg aacacgcaaa gcttcttcga aacccgtcaa actggtaggg
     3181 acggctggcg agagcccaat cagttctcgt tcgacaagat tgggcgtgac gatcggattg
     3241 acgtcgaata cgcgcccgag ggcgtgtcgt gccatcgacc gatgggcaac gacgccacca
     3301 cggtgggcgt cgacaaaaaa tctatgcatt ccgtatggtt ggacagtatc tacctgccaa
     3361 gccagcatct cgtcggatgg ttcttccgca agaacgacca ggctggcatg agccagaggg
     3421 ggcggaagcg gaacgtggag cgctgcggct acctcggcga tggcatcgtt tgcggttagg
     3481 gtaggctggg ggtcgacgcg cagaccgcgg gccacatcga gggcgacgac gcgaacggag
     3541 ccttcggggc cgacgcgaac gacagcgtat ttaccgatga cgggaagatt gtggtggtgt
     3601 tgggcgaagc gaacggtgtg cagtgaccgc aggggtagca cgcgtttggt ctcgagttgg
     3661 accgactcga gtcgaagctg ggacgagtgt ttttgcagat aggtgctcgc ggcggtggtg
     3721 ggatgattcg tatggtttcg aaacgaaggg gagccttgat accattcggt cgcatgggcg
     3781 caaacggata gggtgaggct caagcccgtt acgaataggc cgagtggggt cgtttttttc
     3841 atgaggtgcg atttcctctt gccgtggatc gcgacgcacg aaatggagtg gttcgcatca
     3901 cgcttgcgaa ggtgccgttt tggattgggc ggactacgac acacgtcgca tcaaagagcg
     3961 tagcgtcgcg caagaacaga gcaaggcttt cctttgccgg ggcgaagaaa accggcggat
     4021 ggttcgttgg cattcccttc tgggcggggc cgtccacgga ttgctttttc tcatgcctat
     4081 ctctgatcca ttcgcgtggt attcgagtag gcttgcgggc catggtcgat gacatcaaac
     4141 ggacccttcg agactccaag gctgcccgct ggactgcctt gttgatcgtg tcgttttcga
     4201 tgctctgtgg ctactacgtg gcggatatca tgtcgccgct caagcccatg atcgagcagc
     4261 agcttggttg gagcagttcg gaatacgggt ttttcacggg ggcgtatgca tggttcaacg
     4321 tgtttttggc catgctgatt tttggcggcg tgattttgga caagaaaggt cctcgatttg
     4381 ccggtgttct gggatgtgtg ctgatggtgg tgggatgcgg cgccaagtac ttcgccatca
     4441 gtaccgattc gttgcgtgat gtcatgatgc ttggaaccaa agcccaggtg ctgattgcgt
     4501 ccgtgggttt tgccatcttc ggcgttggga tcgaaatctg cggcatcacg gcgacgaaaa
     4561 tcatcgctcg ctggttcaag ggctatgaaa ttgccctcgc gatgggattg caggtgtcca
     4621 cggcacgcat tggaaccgct ctagcccttg gtcttggagg accgattgca gcggctttgc
     4681 actccgtggc ggctccggtc cttgtcggtc tgctcatgct tgcggtggga ctcatcgcgt
     4741 ttttctttta tgtggtcatg gaccgcaaac tcgacgcctc cgaaacggcg gccgaagctg
     4801 aacccgagga gcctttccgc ctggcggata tcggccaaat cttgcgtatt cgaggctttt
     4861 ggtacatcgc catgctttgt gccctgttct attcagccgt ctttcccttc ttgaaatacg
     4921 cccccgacct catggtgcaa aaattcgacg ttcgcgacga cctcgccgga gtcattccaa
     4981 gcatccttcc tttcgcaacc atcccgctta ccgtcatttt cggcggcttc tacgatcgca
     5041 aaggaaaagg agccacgatc atggccctcg gttccctgct gctcgtcgtc gttcacctca
     5101 ccttcaccat ccccatgctc agtcattggg tcatcgccgt ggttgctacc gtcgttcttg
     5161 gtttcggctt ttccctcgtc ccctcggcca tgtggccctc cgttcccaag ttcattcccc
     5221 aacgacagct cgggaccgcg tacgcgctca tcttctacat gcaaaatcta gtggctctca
     5281 tgggagtccc gtatctgatt ggctgggtcc tcgatcggtt ctgcgtgatc ggaacccgtg
     5341 atgtcgaagg ggtttcgagc cctgcctacg attacacatg gcccatggcc gttttttccg
     5401 cgctcggcgt tctcgcggtg gtattcgcgt tgctgctcaa agccgaagac aagaaaatgg
     5461 gatacggtct ggaactcccc tgccacaaca agtagcaaag atccttcaca gctatcgatt
     5521 cattacatcg acgagcaggt gccgtctttg aacgaagccc gcgagctgga aggaacgagc
     5581 tgcaacaagg aatccggagg accactgcaa tcgggcatta gctgtaaccc gttgatattc
     5641 cacaggtaat gacgtcccgg ctccagttgc acatccttga tgatgtccca gcaaggggaa
     5701 gaaatgaacc aaggcttgcc gtcctctccg tgctgcccga aactcgctgg gaaactgtaa
     5761 atcgacgagc ctggagagca taccggggcg atgatcgtcg taccatcgac aggtccaatc
     5821 caggaagcca tcagggtgac gggctcgtcg gtaggaactt cgagccagaa aaaagcgccg
     5881 aaattctggg atgggaaagg cttttccaca tcgtctttga tcgggggcaa gcagcctcca
     5941 cttcgctgcc tcgtggggta agagtgattg cttatctcat aagtgtaggc ggatttattt
     6001 gaaggatcgg tgctgagcac gaccatcgca tctgactgtc cctcgttttc gagcgggtct
     6061 tggccttggg caaatacctt gagtgtcaag tgacgggtat tgatggggta tggcatccaa
     6121 tccgcgccat aaaacgggtc tttctgccgc aagctatcgt agtcgaacgt tttggtgtcg
     6181 cagggcacag gattgcccgg acgctccgac gcgacgtttt tctcactgta ctgattgggc
     6241 tgacagacgt ttggggaagg ggaatgggga gtggcatccg ttccccaggt ggtgggagga
     6301 aagtctcccg aagcggtcca acaatcctgg aatcgttggg cttcaatgga gcatttgcct
     6361 tgtccaaggc gcttttgaca gagccacaac catcggtttt gcgcatcgca tgccacgtgg
     6421 atgttgtgcc agaaacccca gaggtcgccg tggatatacg caggccccac gttggtgcat
     6481 tccggcgaat tgccttcttc cttgccgaca tcttccattc ctgtctcgtt cgaatcggcc
     6541 aagccggact cttcggatga tgtatcgaca atgttgtctt gcacgctggt atcttgtacg
     6601 ccgctatctt gcgcgctgct gtcctgcaca tccctgtccg tcgtgcctgc tgcatcattc
     6661 ggataggcag tatcgtcgaa cccgagatcg tcgaatccga ggatctgaga acagcccgca
     6721 aacaagacga aaaacagagc catgggggtg tgatgaagct gcacgagtcg attctagcgt
     6781 gaatcggaac aagatgcagc gtgtcgtgtg aggcagccag gcttcccgtt agaaatagcg
     6841 gatgaagtca ggagcaaggc tccgtaaggc gtgacgggta cggcaggtcg atgacccaac
     6901 caggccgcac gaatactact tgggtcttga ttttgtctgt ctccacacct agcagccgcg
     6961 cgacggctct tcgatacgct tccaattgcg ggcgataccg ctcgactttg tccgcaagcg
     7021 atttttcgtc taccgtatcg gttttgtaat cgagcacctc ggcgtccatc actcgatcac
     7081 catgttttcg cacgacgagc cggtcgatca caccagatag caaactgttg tcgtcgtgaa
     7141 gagcaaaagg cagttctcga tgtaattcca ccgaaatttc ggggagttga tacgtctgct
     7201 tcgataacac cgcgtggact tgtggcatgg cgatcatgtc ttggaactga cgtaccaaag
     7261 ccctgacatt cgattcattg gctccggctt ggatggcttt tcgatgcagg atttcgtcgc
     7321 gtggctgctg ctcttccagc caggtgattt gctcgaaaag cgcgtggatg atggaaccca
     7381 tgcgcagcga actcgtgtcg agatacaaca aatcggatgg cacaaaaagc tttcctccct
     7441 cgagggacga tggactggtt tttggcaatc ctttggtgcg ggcaggcgac gaaagcatgg
     7501 taattcccgc aatggcaggc tcgtgtggca aggcctccgg tggtgggaag gtcagggttt
     7561 gaaaccaggt ttcatctcca tgctcaaaca gacatgcgcc ggcagatgcg gcctttccag
     7621 gcgctaaagc atgacgaatg acccccgcga acgtggcggg gatagatgca gagccggtcg
     7681 gttcgatcag catgaaaagc gcatgcaccg cgcgagtcag ggccacgtac aacagactag
     7741 aagactcaca cacctgtccc gcgcgccaac gcttcaccaa ctgcccaagc tccggaagaa
     7801 gcaactgctc ttcctcattg acataaggca ccacccgcgt ggcaggggag atggcagaaa
     7861 ccgagtccag caacaccgcc gggacgggcc ccacaagctg cttgtcaaga tcgggaagaa
     7921 tcacaatgtc gaattgaagc cctttggcct tgtgaatcgt catcacccgc acgtgactcg
     7981 tcgcaggatc ttccacccgt tcggtggacg caaactcgat gaactcatcg attcgcaaaa
     8041 aggtgcgctg gtctagctga ttcgccagcc gcagcaattg ggtgaggcgg cgagcttcgc
     8101 gcgcgtcaca atgaggcgcc accacaagag accatcgtcg aagacacgtt tcaaagccat
     8161 cttgttgaat ttgttgacga attttctgac ttaccgccac agcgctcccc ggatcgtcgc
     8221 gcaacccaag agcttctccc aacaccgagt tggccaagtg gtgccgtgcg atggaatcac
     8281 cgggatggtc ggccaagcgg agaagggaaa gcatcaatcc gacagccgcg gaatccgtga
     8341 tgggattgcc cccctcttcg cttgcgggaa tgcctttttc cctcaataca gccatgactt
     8401 ttcgcacggc gtcgttggtt cgtgtcaaca ccgcgatggt gacgccaggg caacgcttga
     8461 cccattgttg aaccaaatcc gccgcttgtc gcacaggggc cgacgcctgt tcatcgaagg
     8521 gggcgacttg taggcgaacg aatccgggta agtcggtttt tgccgtcgta tggcgttgga
     8581 aacccgcttg ccacgcccgg gcagcggctt gctcctcccg aaacccctgg ttgtcaccaa
     8641 gcccctcgaa gacgtcattg accgtatcga tgatgggctg tgacgatcga tagctggtgt
     8701 gcaaagggac ccaagtgagt ccaggtagat cctgatcgag ggaatcgaaa atatccgcca
     8761 cgcccccccg ccagccataa atcgcttgtt tgacatcgcc cacgagcaat acggaacgct
     8821 gcatcgatgc gtgagctgcc acctcctggg ctagcggccg caatacttgc cactgcgata
     8881 gcgaagtgtc ctggaactca tccagaagca agtgatggat tccgcagtcg aggcgaaaat
     8941 acacctcatc cagacgcccc agcagcgaag cggtggcaag ggcgcgagca acatcgccga
     9001 atcgatacga cgcctgcgtt tgtttgagca ctcgcagatg ctcgtcataa tcagtcaaaa
     9061 accgctggat ggctcgtcca cgcccaatca atgcttcaag aaaaatggtt cgaacctgct
     9121 cgagaagttg ttgcagcaga tctttcaatt cctcgggtat gttcgctttg cggtaggtcg
     9181 gctgcccttc cgcaactttc cgtccaagcc ccgcgtggac gaattctctg aaacgtttcg
     9241 cgcgaacatg ctcgatgctt tgcgccaacg ccttgcttat agacccaggc agcgtcattc
     9301 gtgtttggca ttcttcgagt tgccgcaaaa tcgactcgat cttcgttggg tccgatggcg
     9361 aatccaaggt caacgcttgc catgcaaagg cgggcgcatc gagaaacgtg ccatgcaaag
     9421 aatcgacgat ttccgaaacg gtggcgacca ccggtcttac gtactgccct ttggaaagca
     9481 agcgcacgag tgccaccatg tcttgcacgt cgccgcgtag gctggcttcg atggctcggt
     9541 tttgcatggc atccgccacg gacgtagaaa cgatttgcca ttgcgcgggg aaccccagtt
     9601 ccagcgagaa gctcctggcg atgctggaaa aaaatgagtc gatggtcgac acgcgcaatc
     9661 gatggagcgc tccggtgacc gaacgaagaa cctgtcccac gcgcgcggtg gtgaggtcgg
     9721 gcacaccgat ttgttttccc agctcaacgc taagtgatga atttaaagaa gcttttgcaa
     9781 gacgcgtgag cacgcgcccc aggatttctc ccgcggcttt gcgcgtgaac gtcgtggcca
     9841 aaatcgtctc cggtgcagca cctcgtatca gcaatcccaa gtagcgattc gtgagctgat
     9901 aggtctttcc cgatcccgcg gaggctcgaa tggccacatg cggcgacgtc gccagcgcac
     9961 tcatgaagta cccccttgca gcacggcgcc gccaagccgt tgtgactggc aaatcacgtc
    10021 gaaatcatcg aagaaaggac ggtccgtggg aggccagaac acttgcttgc gaaccgatcg
    10081 caccacatgc tcagccacct gcatcgcact tgccaattgc tgttcggacc atcccgccaa
    10141 cgcctcttgc acctcatggg ctgcggaaag cgatagataa ccaagctcga tctttccgcg
    10201 ggccatcgcc tgcaccattc gatgatatag aggcagttgc aggtttttcc attcgccctt
    10261 cgtcagatga tccttgaccg gatcccgtgg ccgttgagaa accttgtagt cgaacacata
    10321 taaagttccc gtcgactcat ccctgtcgat tcgatcgatt cgacccacca ggtacatggg
    10381 cgtatcgtcg acgttcagcg ctatcttccc cgcatcaatc tcatactccg catgaaggat
    10441 ccgtttgccg cttgccgccc attgcgcttg ccacctagca aaagcgtgca atcgggcgcg
    10501 aagctgttcg acttgcaccc ataccgatga aaaggcctgg tcgccgaacc atgaccgcgc
    10561 ccattcgtcc agccgaacgt cgaggtactc tcgaatcaag ggcacctccg tgctcgtcgc
    10621 cacctccgaa tgcccaaaat ccttgagcac ctcatgcgcc aggttgccaa accctctgcc
    10681 atccaattcc ttggcatcgt cttttgccgc acgaagcccc aatacatgcc gaagataaaa
    10741 tcgataaggg caagataggt aatcaccgaa agcggtaacg gtcatcgctt cgatgggatg
    10801 ggacagcggt ttgggtcgaa cgaccggcaa gcaaggccct tggcatcccg gttgaatgag
    10861 gcttgcgacg ggcagcactt cgctgctccc ctcataaaat gcaagaacat gttccgctat
    10921 gcgctcggtg gaaccgcata acgcaagacg actcggtgcg agcggatcgc ctgccagcga
    10981 acgacgtccc gccatcagcg tgaccgattg ccgggaggcg agcagcgccg agagcaaaaa
    11041 cgcatcgcgt gcaaaacgaa gtcggttgtc cgcaagaccg agatggtgtc gcaaaccatc
    11101 cggcaaaaac ggatcggcat tgcgacaacc aggtaccatg ccatcgttca ttcccgtgac
    11161 gatcaccgcg gcggtatcgt cgtggggaag ctccaaccag ccaagcagct cgatggcctc
    11221 tcgctccgct ggctccggaa tggcaatttc cgccacacta tccacgacaa cccgcaacgc
    11281 ggtggccgcc gacaccttct caacgacaga atcgggcaac tcggccatgg tgcgcaaaac
    11341 gtcttggaaa gccacgagaa tggcgatcgt ttgcttcgac gctgcgtccg tgggatctaa
    11401 tggtttttct ccaaacaggc ggatgaggaa agaagcgatc gcaggcgccc aacgacccag
    11461 ggggcgctcg gaaccgagca agtcagcgag acacgcatgc agcttattca agcgacgaac
    11521 aagcgattgc gcgatgtcgg aggaccccac gaaatggtcc gtcaggttgc aagcaatgtg
    11581 tttggattgg tagatgtcca gaagaactct ccaatccttg tcttgctcct cggattccac
    11641 cagaaggctc agatcgggga ttcgtgcgag aaccccgagg gtggaaaaac tttgtcgttc
    11701 caagtggtta gcaatcgcaa gcagcaggcg cacgacggaa cgacgaagca tcggggtgcc
    11761 cgccgcattg cgcgtgggca cgccgagagg ctcgagggtt tcgctcaaca atggaatcac
    11821 atcgacgtcg ggaacaccga tcaccacgtc ttgtagtccc ttatgagggg aaagttccgc
    11881 gagggttgcg gcaacgcaag ctgcctgctg ggctggatca tcgacgatgt tcaccgtgac
    11941 ctcgccgaga gggccttgct cccacgcgga aggaagaacg gcaccaaatt ggtcgaagcg
    12001 gtctgcaagg gacgcggggg cgtggatgag agcgagcacc cgtgcgcggg tcgcatcaag
    12061 aattttccgt gtaaaaacag taagttccgg acagcccacc agcacgatgg tcgtggccgg
    12121 aggggcaagt tcgacgtttc ctgtcgcgat ggcatcgaga tgacgtgccg tagcgtcatc
    12181 gattcccaac cgctgaaaat gctcatgcac cccttcgcgg atttgagcga tggcttgcca
    12241 acggtcgccg tctcgaaacc cttcgagatg agccgctcgc gcgggaacgt cgccgatgcg
    12301 cagcgccgcg gaagcaagtt ctgcgtcgag atcatcgaac atccggccca attgcaacca
    12361 ggcaggccaa tcgttggggg cgggaggcgt ggggacgagc gtttcaagca gggaagggga
    12421 ggccgagcga agcgcttccg cccagacgat gcttcgggtc aacggggcag cggtgggttg
    12481 gcaaggttgg atgagcgagt cggaaagcgc tcccatcgtg atgacgcgag gtgggaggag
    12541 ggctcttttg ggtccggaat gaaggacaag ggcttcgagc agtctcgcac ctgcccgact
    12601 cgccggggta accaccaaaa aagagcgaag gtcgatgggg tctcccacgt cctgggcgag
    12661 gaaatactcg accgaaaggg ggagcaaagg tcgatcccaa ccaagaaaaa cccgctgaat
    12721 ttcgctttgg cgggcagaca aaccaaggtc gagttgcaca ggctgggcgg tatgagagcg
    12781 gggaggcatg gcgggtcacg cctatggtta cacgaagggg caagtattcg aaacacggtc
    12841 gggcgttatc ggcctttcct ttcaatcgag atgtagatat gaaaactgct cgaaatcttc
    12901 agctagatcc caactgagaa ccgtgagatc atgatcgcat gccatgggtt gatccggtgg
    12961 ctgtatccat gcggattggc gttgttcggc gatggcatat tgcatgcttt gcgagcaacg
    13021 agacgattcg aaggtgtgaa ggaatgtcta tgcgcaagta ttttcacgcg ctgggattgc
    13081 tgcttttgat tgcgttgatg gccaagccca gtcaggccgc ttcggtttgt atcgatccgg
    13141 gccatggcgg gtcggatcca ggtgcgcaag gttgcgggtt gtccgaagcc aaaatcaacc
    13201 tgcaggtttc cttgaagctg cgagatctgc tgaaagccag cggtgttacc gtttatatga
    13261 cacgagaaac ggacgttttc gtaggcttgt cggcgcgagc ctcgtacgcg aattccaaag
    13321 gcgtggaccg tttcgcgagc gtgcattcca acgccggagg gggaacgggg atcgaaacgt
    13381 tttgcatgga agggtcctcg tcgacgtccc tgggctataa aatggcctcg gcaattcaga
    13441 cgcagatgct gaaggtatgg ccgctgccgg atcgcggggt caaaaagg
//
LOCUS       DAOSOR010000088        13484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4593_length_13484_cov_4.435401, whole genome shotgun sequence.
ACCESSION   DAOSOR010000088 DAOSOR010000000
VERSION     DAOSOR010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 13484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13484
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4593_length_13484_cov_4.435401"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PK710_06810"
     CDS             complement(<1..643)
                     /locus_tag="PK710_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09460.1"
                     /translation="MSSVPSNKTPGGKPGSAQGAPRPAGAIPRPGSSAAQPAHPATRR
                     AVNSPQKTILGMPSPVVPVGATPMAPAQPVARPTPAPVQSASAKAPPPPVPAKPPDPM
                     DDDDFDEAPTAMFVHSVKYKYDPDAPPEPGSPGLLDDDDDDEYEPPTAIHVSPYRGDG
                     PEPFGQIQRPAAMAPASPPTAPSEAKPVPAAGAPAVRPPFTPPGVIAQPQPQLQ"
     gene            complement(731..1219)
                     /locus_tag="PK710_06815"
     CDS             complement(731..1219)
                     /locus_tag="PK710_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT09461.1"
                     /translation="MADLDSLLVRAKNEAVARRFEEAAALLEEAYAADPNDPRVLDLF
                     GFVRFFQGSFAEAEAHCRNALRVDPDRAYAHKGLGLCLARQGKIEEGIVSIERAITLR
                     PSWFDPYWDLAVVLSDVGRFDEAIDILAQGAAMVPSRQKDFRKFQTSLRQRKAESLSK
                     ER"
     gene            1405..3012
                     /locus_tag="PK710_06820"
     CDS             1405..3012
                     /locus_tag="PK710_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HOT09462.1"
                     /translation="MTASSWYDLAGMACFLLLSTRKEGNGARCSKLGSIIRQAGMASK
                     KIRFPTIMRAAIGLLSLIQIANLLHLFRLRVAYPYQLEWMEGAVLEHIERVSTGQSLY
                     VAPSVSFTPYLYTPFYYAVSGAMCRVLGFGLEAPRVVSIVATVATLGFVASFVYEETR
                     DRLAPLVAVALFVSTYEVTGFWFDLARVDSLFHCLVLASLWLARFGKSHGAMAGAGAT
                     LFLAFFTKQAALAFLLPLAVFAFATGWKHALNAIASFSVLTVTATLYLQRISEGWFTY
                     YVFRVTGQHKVHWDWLAPSFLEYFIKPYGIALLFTVIVLVIRPRALQRRRLTAAYVLW
                     LLCAVASSALSLNHQDGWVNVLIPAYGALAVVAGFGFGAAQRNRHQSIVVMSIVMWGI
                     AFAVLVYPPSRALPTRADWAAGALVTQALRSAPAPLWMPGAGYLQALAGHEENSAHTM
                     ALADIFKTQEPRIRNQLRDDILVSVRQHRWASIVIDPSFRMLPPEIMEEIRRCYRWHQ
                     SIFPEHEHGGWPNTGFQTRPQEIWIPR"
     gene            complement(3339..4034)
                     /locus_tag="PK710_06825"
     CDS             complement(3339..4034)
                     /locus_tag="PK710_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09463.1"
                     /translation="MTEPIGYSTAREKNYSSIDDEPLVCTSDAPNATGCSQTARKADE
                     PMSVCCLEELASHPFPTPSFGAFGQSEPPSGDNPNDGAHGSGQAPIAARTPPAPTKAG
                     PRSGIAIGWELSAAYGDIAGVSYSVGQGLLFHEKGVSQYETGMPAGPLGLPMVLSPAV
                     LGASAGIGFSLQYVHDIDAFDGQGVEFSGDYNGATLSVGFSSDHRLSSLGVTLGASVG
                     VAAHARQTETHVK"
     gene            4284..5306
                     /locus_tag="PK710_06830"
     CDS             4284..5306
                     /locus_tag="PK710_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT09464.1"
                     /translation="MEYRRLGSSGLKVSVLSLGAMTFGESKTFMKGVTSTDDQARRVF
                     DAALEDGVNLVDTANVYGDGASERMVGAWIKGKRDRVLVATKCRFPFDMGLRQPGPHD
                     QGLSRKHIMEACEASLRRLDVETIDLYQVHMQDRSVGIEETLRALDDLVSQGKVRYIG
                     CSNYTGYRLVEALSVAERRRTVRYESVQLQWSLIVRGAEREVIPACEAFGVGVMVWSP
                     LARGFLSGKYQKGMAPPPGCRLEQWKESFQLLDQPPNWRILDKVQQLAALHETTPAAV
                     ALAWLLAKNAVSTIIVGARTVEQWKDNRRALDLVLTAEQVRALDEISAPPWGYPYDFI
                     GSREPW"
     gene            complement(5356..6342)
                     /locus_tag="PK710_06835"
     CDS             complement(5356..6342)
                     /locus_tag="PK710_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09465.1"
                     /translation="MSQPRPVVIRIPRPYDTEDEFIRGDGLAIGRLSMILIGAPSRPP
                     GITIRFEIVLRNGEPIFRGEGRVVALRVHQNGREGLEIRFTRLDSRSKALVEQVLALR
                     RSGALTPVSMPPPSARDATNARAREFEVPRLTSPRQAATSPATDHVLDSTELVPIEST
                     PSPAVDTVAAVTKTASLGLPSASAKQPSSPASENLQAPLDEDPTPFAPPVALEQFEQQ
                     AEGATDTKDTEPPSAAQDTPEQAFETRNEAQTKNIPSAEDESVKPQPMPEKPAASHSA
                     KIAEHIESIEQSFDATSLKEALSRLRTRAGTFDSPPAGGEVLDRLRNKTAAS"
     gene            complement(6495..6926)
                     /locus_tag="PK710_06840"
     CDS             complement(6495..6926)
                     /locus_tag="PK710_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09466.1"
                     /translation="MPLLRRQLLPLPLLLLVPGTLGGCKSAGIREVYTSRDAAGRLRT
                     QYFADDKTEIHVIIQMVSGREDAVLYVDLFVPEGSQAKLDRVEIAPGKGDHRIDLRLI
                     MEDELHGTKDDDGPWPTGKYEVDLLIDAEYQETVVFYVGEV"
     gene            complement(7019..7780)
                     /locus_tag="PK710_06845"
     CDS             complement(7019..7780)
                     /locus_tag="PK710_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOT09467.1"
                     /translation="MIDDDLRIHKQGAALIATIDRPSTRNAIAKRTAEQIVSFLHQAS
                     NDESVRTAIMTGAGQEVFVAGGDLHEFDSYTQMQNGASHVLGMGIMTAAMEACDVPVI
                     AAVQGAALGGGCEIILACDLVIAEQHARFSFRQAAMGLSTGWGGGTRLLERVGPMRTA
                     QWLLSGATIDANEALQNGLVANVVPKGSALQHALTWTDKVAQLPRASVAGLKRMIHTV
                     RQEMRGHALQREARVFSSLWAKPDHLAAMKAFLAR"
     gene            complement(7777..8745)
                     /locus_tag="PK710_06850"
     CDS             complement(7777..8745)
                     /locus_tag="PK710_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HOT09468.1"
                     /translation="MRIAILSRNASLYSTSRLALAARSRGHKVLIIDPLELHLVVSDA
                     KPALIYGSEPLGRVDVVIPRIGTSINSYGLTVVRQFDLMGVPVLNPAVSIARSRDKLR
                     SLQILTAKKIAVPVTVCARSPAAVKSALEIVGGCPVIVKLQQGSQGIGTMIAETPQAV
                     MSLLETFWAMGQDIILQEYIRESKGRDVRAVVVGHRVVAAMRRTARAGEFRSNLHRGG
                     VAVGVHLDPAYRAIAIKAVRLMALEVKNVDMVETRSGPKILEINASPGLEGIERASGV
                     DVAGAVIDYAERFVTRRRGQGRRQAVSRLSAVVDDERVRIRKRGVS"
     gene            9176..10147
                     /gene="gshB"
                     /locus_tag="PK710_06855"
     CDS             9176..10147
                     /gene="gshB"
                     /locus_tag="PK710_06855"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HOT09469.1"
                     /translation="MRFLFVMDPLETMHPEKDTSFAFMRAAQKRGHTIVHCLLQDLFV
                     QEGKAHAAARTASVRPTAPFSSYGESQLLSAHDVDAVLIRKDPPFDIAYLNATQILDL
                     ARHDTFIMNDPRGLRDADEKLFALHFSSWIPRTCVTSDSQRIRTFLGECGGAAVIKPI
                     GRAGGFGVLKLTSQDINTPSIVSLLTEEGKRPVMVQEYLPSVRQGDKRILVLDGKPLG
                     AILRVPRDDDLRANIHVGGSVVGSDLTAEEVALVADVGPVLRAAGLYFVGLDVIGGRL
                     TEVNVTSPTGIQELGRLNGTSPEDEVIEWLESKAEEIAKGRRARQGL"
     gene            complement(10144..10710)
                     /locus_tag="PK710_06860"
     CDS             complement(10144..10710)
                     /locus_tag="PK710_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF99 family protein"
                     /protein_id="HOT09470.1"
                     /translation="MSFGPRSRIVGIDDAPFVRRTGARVPLIGVLMSGWRVHGVMRSQ
                     IVRDGFGATARMIAMLRSGRFANQAQAVVLDGIAVGGFNIVDLPTLAESVGVPVIAVM
                     RHRPNFDAIRQALNKTRQAERRWRTMMRAGEIFESNTLFFQVAGASDREARTILAHAS
                     EHGGYPEALRIAHLIGGALQKGTSRGGA"
     gene            complement(10712..11410)
                     /locus_tag="PK710_06865"
     CDS             complement(10712..11410)
                     /locus_tag="PK710_06865"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT09471.1"
                     /translation="MNLVLPPVPENPVRPPMVDLPGEEPFRIDTVDGHSLEARLRLPS
                     DARRAVVLCHPHPLYGGTMHNAVIVAVAKTLAQADEPTAVLRFNFRGVERSSGRYSEG
                     QGEKLDVLAAIDGLRNMNPRMDISVVGYSFGSWVGMRAAWEHPEVERLALIAPAARVF
                     SYVEDQECRRPLPTFIAVGDQDGFASVDSVRKLASYLGAHLDIIHGADHFFVGYRRTL
                     ATRLMPFIAPTMGK"
     gene            complement(11407..11868)
                     /gene="trxC"
                     /locus_tag="PK710_06870"
     CDS             complement(11407..11868)
                     /gene="trxC"
                     /locus_tag="PK710_06870"
                     /inference="COORDINATES: protein motif:HMM:NF008229.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HOT09472.1"
                     /translation="MADEPTTTIECDSCEAVNRVPLNKLRNRPKCGKCGAKLPLGGGP
                     ITLTDANFERTISTSPVPVVVDFWAPWCGPCRMIAPALEKIAGEMSHDVLIAKLNVDE
                     NPVVASRFQVRSIPMLIGFADGEAIDTQTGALPPPALRSWVKGVVDKASLS"
     gene            complement(12052..12420)
                     /locus_tag="PK710_06875"
     CDS             complement(12052..12420)
                     /locus_tag="PK710_06875"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOT09473.1"
                     /translation="MNRRSLTPPTGIQGILAQRRAAGGARREISARLILHTGNAQYDG
                     WALNISRGGIRVVIEGRVHLGQVLTIEGYDPDREDAPPRRARVIWSQDEPDGSVVGME
                     FLDPDDSMPPPTVTPKSPTK"
     gene            12616..13053
                     /locus_tag="PK710_06880"
     CDS             12616..13053
                     /locus_tag="PK710_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09474.1"
                     /translation="MGELTLSDIKQLLIAHGMIVYRMTGNSVAIAERVRDNLIMEAHV
                     QVVVESGMRVRFTTRAQRADFPSSSETEPTLLARARELGKPALSLGFSEICTTVESIS
                     DPMDASCSLDTWYQVHFEKLVNTDEELICAVQQAMVVKKVAPR"
     gene            13171..>13484
                     /locus_tag="PK710_06885"
     CDS             13171..>13484
                     /locus_tag="PK710_06885"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOT09475.1"
                     /translation="MSFVYGGIVLAIAMVFIVQFRPGSGRETASLTRQCAVTIRDRCI
                     EPKEFLASLGLAAPRGADDDRLRAMQIRRQVAEGLIERTLLVQDAKRLAMTVSDDEIN
                     AEL"
BASE COUNT         2785 a         3655 c         4059 g         2985 t
ORIGIN      
        1 gttgaagctg gggttggggt tgggcaatta cccctggcgg agtaaaaggc gggcgaacag
       61 ccggtgcgcc cgcggcagga acgggcttag cctcggaagg ggccgtggga ggggaggcgg
      121 gagccatcgc cgccgggcgc tgaatttgcc cgaacggttc gggaccatcc ccccggtaag
      181 ggctgacatg aatcgccgtc ggcggctcat attcatcgtc gtcgtcatcg tcgagcaacc
      241 caggcgaacc cggttcagga ggcgcatccg ggtcgtattt atacttcacc gaatgaacga
      301 acatcgcggt cggggcctcg tcgaagtcgt cgtcgtccat ggggtctggc ggctttgcag
      361 ggaccggagg aggtggagct ttggcggagg ccgattggac gggagcaggg gtggggcgcg
      421 caaccggttg cgctggtgcc atgggtgtgg cgccaactgg caccacgggg cttggcatgc
      481 ccagaatggt tttttggggg gaattgacag cgcgccgggt tgcagggtga gccggttgtg
      541 ccgccgacga cccaggtcgc ggtattgcgc ctgcggggcg gggagcgccc tgagcagaac
      601 ctggctttcc gcctggggtc ttgttggagg gaacagaact catcgatacc agtatcctac
      661 actctttatt tccgactcga agtgcaagct tttcgagctt ggttccggct cgacggacgg
      721 tttttggttt tcacctttct ttcgaaagcg attccgcctt gcgctgacgc aggctcgttt
      781 gaaacttccg gaaatctttt tgccttgaag ggaccatggc cgccccctgg gccaagatat
      841 cgatcgcttc atcgaaacga ccaacgtcgg agagcacgac cgccaagtcc caatacggat
      901 cgaaccagga tgggcgaagc gtgatggcac gctcgatcga aacgatccct tcctcgattt
      961 taccttgccg agcgaggcat aaccccaatc ctttgtgggc ataagcgcga tcgggatcga
     1021 cacgcagggc gttgcgacaa tgcgcctccg cctcagcgaa ggatccttga aaaaacctaa
     1081 caaatccaaa tagatccagc actctcggat cgttggggtc cgcggcgtac gcttcttcga
     1141 gcaacgccgc tgcctcttcg aagcggcgag ccaccgcttc atttttggcg cgaaccaata
     1201 acgaatccag atcagccatg gagacaactt agcattgatc cagtcgcagc gtcatcttct
     1261 gagcgacctg gcaccacatc ggccatgcat tcatttcgaa aaggcaacat cggctccccg
     1321 ccgcatcacg gaccagcgat gtgattctcg tccaaagagc atagcagcca gaaatgcaga
     1381 aagacacgag acgagcaacc tctcttgaca gcgtcgtcat ggtatgactt ggcaggcatg
     1441 gcttgctttc ttctcctgtc gacgcgcaag gaaggcaatg gggcaaggtg ttcgaagctt
     1501 ggctcgatca tccgacaagc ggggatggca tcgaaaaaaa tccgatttcc gacgatcatg
     1561 cgggctgcga tcgggttgct ctctctgatc caaatagcga atttgctgca tctatttcgc
     1621 ttacgagtag cgtatcctta ccaactcgaa tggatggaag gcgcggtgct cgagcatatc
     1681 gagcgcgtct cgacaggcca atcgctgtac gtagccccgt ccgtttcgtt cactccgtac
     1741 ctgtacacac ccttttacta cgcggtcagt ggggcgatgt gtcgggttct ggggtttggg
     1801 ttggaggcac ctcgtgtcgt gtccatcgtg gccaccgtcg cgactctcgg cttcgtggcg
     1861 agtttcgtgt acgaagaaac gcgcgatcgg ttggcgcctc tcgttgccgt ggccttgttc
     1921 gtctcgacct atgaggtgac ggggttttgg ttcgatcttg ctcgcgtgga cagtctgttt
     1981 cattgtctcg ttcttgcgag tctatggctc gctcgatttg gaaaaagcca cggggccatg
     2041 gcgggggcgg gggctacgct gtttttggct ttttttacga aacaagcggc ccttgccttc
     2101 ttgctacctt tggccgtctt tgctttcgca accggctgga agcatgcgct caacgccatc
     2161 gcttcctttt ctgttcttac cgtgacagcc accctgtatc tgcaacgcat ctccgaagga
     2221 tggttcacct attacgtgtt ccgcgttacg gggcagcaca aggtccattg ggattggttg
     2281 gcgcctagtt tcctggagta tttcatcaaa ccttacggaa ttgccctgct gttcacggtc
     2341 atcgtgttgg tgattcggcc cagagcgttg caacgacgtc gtctcaccgc tgcctacgtc
     2401 ctttggctgc tttgcgctgt ggcctcatcg gctctttcgc tgaaccatca ggacggctgg
     2461 gtcaatgtgt tgattcctgc gtatggtgcg ctggccgtcg tggcgggttt cggattcggt
     2521 gccgcacaac gaaatcgaca ccagagcatc gtcgttatga gcatcgtgat gtggggcata
     2581 gcgtttgctg tccttgtcta tcctccctct cgcgccttgc ccacgcgcgc tgattgggct
     2641 gcgggagcgt tggtgacaca agcccttcgt tctgcgcccg cgccactgtg gatgccggga
     2701 gcaggctact tgcaggccct cgcgggccat gaggaaaact cagcccacac gatggctctg
     2761 gcggatattt tcaaaaccca agagccccgc attcggaacc aactgcgcga cgacatcctg
     2821 gtgtcggtgc ggcaacaccg atgggcaagc atcgtgatcg atccatcgtt tcggatgctg
     2881 cccccagaaa tcatggagga aattcgtcgt tgctaccggt ggcaccagtc tatttttccc
     2941 gagcatgaac acggcgggtg gcccaacacg ggatttcaga cacgaccgca ggagatctgg
     3001 ataccgcggt gaagggagaa gcgaaggcgt ttgctatccc gatatgtaca atgcggccat
     3061 gactgatatc gttgacgttt ttgtcaaaaa aatcaattta tatcatatat ttcaatatgt
     3121 tactacgatt ctgccaacgc agccaatacc aacaatacat atccgtatat tacggattga
     3181 atgacgactg gggaaaagtt ggctggtttg taggtcttcc gctttggcga atccacggat
     3241 gggatcgatg tgcctaccgc gggtcgacga actccctcag gctttctgcc agtgcatggg
     3301 gaggcacgaa aaccatggtg ccaaagtatg gtcttcggtc atttgacatg ggtttcggtc
     3361 tgtctggcat gcgcggccac tccgacgctt gcgcccaagg tgacaccgag ggagctgagg
     3421 cgatggtcgg aactgaatcc cacggacagc gtggcaccgt tatagtcccc ggaaaactct
     3481 actccctgtc cgtcgaaggc atcgatgtca tgcacgtatt gcaggctgaa ccctattccc
     3541 gcggatgcgc ctagcacagc gggagacagg accatgggaa ggcctaaggg gccagcaggc
     3601 atgccggttt catattgcga cacacctttt tcgtgaaaca ggagcccttg ccctacggaa
     3661 taggacactc ccgcgatatc tccgtaagct gctgacaatt cccatccaat ggcaatgccc
     3721 gaccgcgggc ctgctttcgt aggagcagga ggagttctgg cggcgatggg tgcttgccca
     3781 ctgccatgcg ccccatcgtt gggattgtca ccactgggcg gttcagattg cccaaacgct
     3841 ccaaacgatg gtgtcggaaa gggatgggaa gccagttctt ccaagcagca taccgacatg
     3901 ggttcgtcgg ctttgcgagc cgtttgcgaa catccggtgg cattcggcgc atccgatgtg
     3961 caaaccaaag gttcgtcgtc gatgctgcta taattttttt ctcgtgctgt ggaataacca
     4021 atcggttcgg tcatgaccat cctctttcat cagtcagacg cgtgaactca cgacgtcagg
     4081 tgggaaggca tctaccgcag gcttcgcttt ggatgcgctg cctgggactt gccaaggttc
     4141 ggacgtcgag gtggattggt tgctggcaat cgatcgactc gtggtatttt tttccttcgc
     4201 tatcccttgt tcatggcagg ggtttcgatg ttccgtgtcg agcatgcttc ccacgagcac
     4261 gccgctgctt tagcaacagg agcatggagt atcgaagact tggaagcagt gggctgaagg
     4321 tcagtgtctt gagtttgggg gcgatgacct ttggtgaatc gaaaacgttc atgaaaggag
     4381 tcacttccac cgacgaccag gcgcgtcgtg ttttcgatgc ggctctggag gatggggtga
     4441 atttggtgga caccgccaat gtgtatggcg atggcgcgag cgaaaggatg gtgggggcct
     4501 ggatcaaagg aaaacgagac agggtccttg tggccaccaa gtgtcgattt cccttcgata
     4561 tgggactccg ccagcccggc ccccacgatc aggggctgtc gcgaaagcac atcatggaag
     4621 cgtgtgaagc atcgttgcga cgtctcgatg tggagaccat cgatttgtat caggtacata
     4681 tgcaagaccg gtcggtgggt atcgaggaaa ccctgcgcgc cctcgacgat ctggtttcac
     4741 agggcaaggt tcggtacatc ggatgttcga actatacggg atatcgtttg gtggaagcct
     4801 tgagcgtggc cgaacgtcga agaaccgttc gttatgaatc ggtacagctt caatggtcac
     4861 tcatcgtgcg aggcgccgaa agagaagtga ttccagcctg tgaagccttc ggcgtgggcg
     4921 tgatggtatg gtcgcctctt gcgagaggtt ttttgagtgg caagtaccaa aagggcatgg
     4981 cgccacctcc gggatgtcga ctcgaacagt ggaaagagag ttttcagctt ctcgaccaac
     5041 caccgaattg gcgcattttg gacaaggtgc agcaactggc ggccctgcat gaaacgacac
     5101 cggcagccgt ggcgctcgca tggctgcttg ccaaaaatgc cgtatccacc atcatcgtgg
     5161 gggcgcggac cgtcgagcaa tggaaggaca atcgtcgggc cttggatctt gtgctcaccg
     5221 cggagcaagt ccgcgcgctg gatgaaataa gcgcgcctcc gtggggatac ccgtacgact
     5281 tcattggctc ccgagagcct tggtagacat ccgatgaccc catgagcgca gtcacgcttt
     5341 tgggtactcc gtatgtcaag aagctgccgt cttgttgcgc aatcggtcga gcacttctcc
     5401 gccagccggg ggagagtcaa aggttccagc tcgagttcga agacgagaaa gcgcttcttt
     5461 caagcttgtt gcatcaaaag attgttcaat actttcaata tgttccgcaa tttttgccga
     5521 gtgggatgcg gcaggcttct cgggcatcgg ttgcggtttc acgctttcgt cttccgcgga
     5581 tgggatgttt ttcgtttgcg cctcattgcg cgtttcgaag gcttgttcag gggtatcttg
     5641 cgcagcagag ggcggctccg tatctttggt atccgttgca ccctcggcct gttgttcgaa
     5701 ttgctcgagc gccactggcg gtgcgaaggg agttgggtct tcatccaacg gggcctgtag
     5761 attctccgaa gcaggcgagg atggttgctt cgccgatgca cttggcaagc ccagcgaagc
     5821 ggttttcgtc acagccgcca cagtatcgac cgcgggggaa ggagttgatt caatcggaac
     5881 caactccgtg gagtccaaga cgtgatccgt tgctgggctc gttgccgctt gacgcgggga
     5941 agtgagtctg gggacttcga attcccgtgc gcgagcgttc gtagcgtccc ttgcggatgg
     6001 cggaggcatg gaaacaggcg tcaacgctcc agagcgtcgc aaggccagaa cctgttcgac
     6061 gagggctttc gaacgtgagt ccaagcgggt gaaacgtatt tcgagccctt ctctgccgtt
     6121 ttgatgaacg cgaagggcaa cgacacgacc ttctcctcgg aagatgggct cgccatttcg
     6181 gagcacgatc tcgaaacgaa tcgtaattcc tggaggtcga ctgggtgcgc cgatcaggat
     6241 catactcagt cggccaatgg cgagaccgtc gccacgaatg aactcgtctt ccgtgtcgta
     6301 gggacgtggg atgcgaatga cgacaggtcg cggttgagac aagtgtcacc tcagtgggaa
     6361 tccggcagat gatagacccg gtagactcga agcgtcagca aagggcccag gaagaaaagg
     6421 ataagggcag tgcgaagaag cgacaaccga aggctcgata agaaccgatt taatatataa
     6481 atatcaaata gttattagac ttcccccaca tagaaaacca ccgtttcttg gtactccgca
     6541 tcgatgagca gatcgacctc gtacttgccc gttggccatg gcccgtcatc gtctttcgtg
     6601 ccgtgtaact catcttccat gatcaaccgc aggtcgatgc gatggtcgcc ctttcctggt
     6661 gcgatttcga cacgatcgag tttcgcttgg ctgccttcgg ggacaaataa atcgacgtac
     6721 aacacggcat cctcacgtcc ggacaccatt tggataatca cgtggatttc ggttttgtcg
     6781 tcggcgaaat actgtgttcg caagcgtccc gcggcatctc gagaggtata gacttctcga
     6841 atacccgcgg acttacaacc gcccagggtg ccagggacca acagcagaag gggtaaggga
     6901 agcaactgtc gacgaagcag gggcatgcga gactcctagg atggatttac atggagtcgt
     6961 gcccagcggc aatgcatggg tgcgatccgg tggcggacga gttggctgtc aaggctggtt
     7021 atcgtgcaag aaaggctttc atggccgcaa gatggtcggg ttttgcccac agcgaagaga
     7081 agacccgagc ttcccgttgc aacgcatggc ctcgcatttc ttgtcgaacg gtgtggatca
     7141 tgcgtttgag tccggcgacg gaagcccgag ggagttgagc caccttatcg gtccatgtca
     7201 gggcatgctg cagggcggag cctttgggca ccacattggc cacgaggccg ttttgcagcg
     7261 cttcgttggc atcgatggta gcgccagaga gcaaccattg cgccgtgcgc atggggccaa
     7321 cgcgttcgag gagtctggtg ccgccgcccc atccggtgga caatcccatg gcggcttgtc
     7381 gaaaggagaa gcgagcgtgt tgctctgcga tcacgagatc gcaagcgagg atgatttcac
     7441 atccaccacc gagggcagcg ccttgcacgg cagcgatgac gggtacgtcg caagcttcca
     7501 tcgcggcggt catgatgccc atgccaagca cgtgggaagc accattttgc atttgcgtgt
     7561 acgaatcgaa ttcgtgcagg tctccccctg ccacgaagac ctcctgtccc gcgccggtca
     7621 tgatggcggt ccgcacggat tcgtcgttcg aagcctgatg caaaaaggat acgatttgtt
     7681 cagcggttcg ttttgcgatt gcgtttcgag tggagggtcg gtcgatggta gcgatgagcg
     7741 ccgcaccttg cttatgaatt cgcaagtcgt catcgatcac gacacaccgc gctttcggat
     7801 tcgaacacgt tcatcgtcca ccacggccga cagacgggat acggcctgtc gccgaccttg
     7861 tcctcgtctt cgggtgacaa atcgttctgc gtaatcgatg accgcgccgg cgacatcgac
     7921 gccactggct cgttcgatcc cctcgaggcc gggggatgcg ttgatttcga ggattttggg
     7981 tccggagcga gtttcgacca tatcgacgtt tttcacttcg agggccatga gccgcacggc
     8041 tttgatcgcg atggcgcggt aggctgggtc gagatggaca cccacggcga cccctccccg
     8101 atgcaaattc gatcggaact cacctgcccg tgcggttctg cgcatcgcgg ctacgacacg
     8161 atggcccacg acgaccgcgc gaacgtcgcg gcctttggat tcgcggatgt actcctgcaa
     8221 gatgatgtcc tgacccatcg cccaaaatgt ttcgagcagc gacatcacgg cctggggggt
     8281 ttccgcgatc atcgtaccaa tgccctgact gccttgttgg agcttcacaa tgaccgggca
     8341 tccaccgaca atctcgagag cgcttttgac agcggcggga gagcgagcgc aaacggtgac
     8401 cggcaccgcg atcttctttg ccgtcagtat ctgcaaagaa cgaagcttgt cacggctacg
     8461 ggcgatggat acggcaggat tcaacacggg aacgcccatc agatcgaatt gtcgtaccac
     8521 cgtcagccca taactattga tgctggtgcc gatacgggga atcacgacgt cgacacgtcc
     8581 caaaggttcc gagccgtaaa tcaaagcagg cttcgcatcg gatacgacca gatgcaactc
     8641 gagcggatcg atgatgagca ccttatgtcc gcgcgagcgc gctgccaatg cgagccgact
     8701 ggtgctgtaa agcgatgcgt tgcgtgaaag gatggcaatc cgcatggggg gggcacgtta
     8761 aacagagaca cgcgctttcg tccacccgca tccacatgcc catcgtccga tcagtttcca
     8821 aaacagggaa aaacaatcta attacgccga attaatcacg cagcacgatg gctcgacccc
     8881 tccatgccac ggaaaaaccc gtgcattccc gagacgattt gtctagccca tgacccgtcg
     8941 ttcagaatcc catcatccat cgtcgccaat accagcggca atcacgatac ccccgggcag
     9001 gctcgccgca ttttgtctca gaccccatcg cccacgaggg ttccccgagt gttttactaa
     9061 ctcgttggca tggaaccata agcccatcct ttcgtatcga tcgtcatgca cgttccgttc
     9121 gctcatcgat cgacggccca tatcggatgg gcgcaacagt gctatcttgc cgtccatgcg
     9181 ttttttgttc gtcatggatc ccctcgagac catgcatccc gaaaaggata catcgtttgc
     9241 cttcatgcgc gccgcgcaga aacgcggcca caccatcgtt cactgcctgc tccaagacct
     9301 attcgtccaa gaaggaaaag ctcatgctgc ggctcgtacc gcgtccgttc gccccacggc
     9361 tccgttttct tcctacggcg aatcccagct cctaagcgcc catgacgtcg atgccgttct
     9421 cattcgcaag gatcctcctt tcgatatcgc ctatttgaac gcgacccaga tcctggattt
     9481 ggctcgtcac gacacgttca tcatgaacga tccgcgcggg ttgcgcgacg ccgacgaaaa
     9541 gctgtttgct ttgcattttt cttcttggat tccgcgtact tgcgttactt ccgacagcca
     9601 gcgcattcgt acgttcctgg gcgaatgcgg aggtgcggcc gtcatcaaac ccatcggtag
     9661 agcaggcggc ttcggggtgt tgaagctcac gagtcaggac atcaatacac catcgatcgt
     9721 gagccttctc accgaggaag ggaaacgccc cgtgatggtg caggaatacc tgccgtccgt
     9781 tcggcaaggg gacaagcgga ttttggtgct cgatggcaaa cctctcgggg ccattcttcg
     9841 cgttcctcgg gacgacgatt tgcgggcgaa tattcacgtt gggggcagtg tggtcggcag
     9901 tgatctcacc gctgaagagg tggcgttggt tgctgacgtg ggtcccgttc ttcgcgctgc
     9961 tggtctgtac tttgtgggac tcgacgtcat cggtggtcgg ctcacggagg tcaatgtcac
    10021 gagtcccacg ggcatccaag aattgggaag actcaacggc accagtcccg aggatgaggt
    10081 gatcgagtgg ttggaatcaa aagccgagga gattgccaag ggacgaaggg cacgacaggg
    10141 gctttaggct ccaccacggc tggtgccttt ttgcaacgcg ccaccgatga gatgggcaat
    10201 tcgtaatgct tcgggatatc caccatgttc cgaagcgtgc gcgaggatgg tccgtgcttc
    10261 gcgatcggag gctcctgcga cttgaaaaaa cagggtattg ctttcgaata tttcacccgc
    10321 gcgcatcatg gttcgccatc gacgctccgc ctgacgagtt ttgttcaacg cctgccgaat
    10381 cgcatcgaag ttgggtcgat gacgcatcac ggcgatgacg ggaacaccta cggattcggc
    10441 caaagtcggc aggtcaacga tattgaagcc ccccaccgcg atgccgtcga gcacgaccgc
    10501 ctgcgcttgg ttggcgaatc gtcctgacct cagcatggcg atcatgcgag cggtggctcc
    10561 gaatccatcg cggacgattt gagagcgcat cacgccgtgg acacgccacc cgctcatcaa
    10621 aacgccgatg agcggcacgc gagcgccggt gcgacgcacg aagggcgcgt catcgatgcc
    10681 cacgatccga ctgcgcggac cgaaggacat cttactttcc catggttgga gcgatgaagg
    10741 gcattaatcg ggtagcgagg gttcgacgat atccgacgaa aaagtggtcg gcgccatgga
    10801 tgatgtcgag gtgagcaccg aggtagctgg cgagttttcg aacggaatcc acggaagcaa
    10861 agccatcctg gtcgcctacg gcaatgaacg tgggcaaggg gcgtcggcat tcttggtctt
    10921 cgacgtagga gaacacacgt gcggcgggtg cgatcaacgc caatcgctcc acttccgggt
    10981 gttcccaagc ggcgcgcata cctacccatg agccgaaaga gtatcccacg acggagatgt
    11041 ccatacgtgg gttcatgtta cgcaatccat cgatggcagc gagcacatcg agtttttcgc
    11101 cttggccttc ggaatagcgg cccgaagacc gttcgacgcc acggaagttg aaacgcagga
    11161 cagcagtcgg ctcgtcggct tgcgcgaggg ttttggctac ggccacgatg acggcgttat
    11221 gcatggttcc cccatacagt gggtgggggt ggcataagac gacagcgcgg cgagcatccg
    11281 agggcaggcg cagtcgagct tcgagcgagt gaccgtcgac ggtatcgata cggaatggtt
    11341 cttcccctgg caagtcgacc atggggggac gaacggggtt ttctgggaca ggggggagaa
    11401 cgagattcac gacaaggagg ccttgtcgac gacgcctttg acccaggagc ggagtgcggg
    11461 agggggcagg gcaccggttt gtgtatcgat ggcttcgcca tcggcgaagc cgataagcat
    11521 gggaatgctg cggacttgaa agcgtgaggc gacgacgggg ttttcatcga cgttgagttt
    11581 ggcgatcaac acgtcgtgag acatctcacc ggcgattttt tcgagagcgg gagcgatcat
    11641 gcggcatggg ccgcaccaag gggcccagaa gtcgaccacg acgggaacgg gggaggttga
    11701 aatcgtgcgc tcgaagttgg catccgtcag ggtgatgggg ccacctccaa gcggcaattt
    11761 tgctccacat ttcccgcact taggtcgatt ccgcagcttg ttcaggggca cgcgattgac
    11821 cgcttcacag ctatcacact cgatggtcgt cgtcggttcg tcagccatgg ttcactcgtt
    11881 ggcaaaaggg gtcgaggttg cgtttttcat gggagccgca cggagtcaaa aagattctgg
    11941 atgcggaatt gtcaaggttc gcgtttcgtt cggcaagcat tcgacaaggg gtggatgtgg
    12001 gaaacgaaag ccgcggaggc gagttgggat ctcatttttc gagggatacg tttatttggt
    12061 gggggatttg ggggtgacag tggggggggg catggaatcg tcggggtcca aaaattccat
    12121 ccccacgacg gaaccatcgg gttcatcttg ggaccaaatg acgcgggcgc gtcgcggtgg
    12181 ggcatcttcg cgatccgggt catatccttc gatggtgaga acttgtccca ggtggacgcg
    12241 tccttcgatg acgacccgga ttcctccgcg gctgatattc agggcccagc catcgtattg
    12301 ggcgttaccg gtatgcaaga tcaatcgtgc ggagatttca cgacgtgcgc ctcctgctgc
    12361 tcttcgctgc gcgagaatgc cctggatgcc ggtgggaggg gtcagacttc gacggttcac
    12421 gacgaactcc tagcacgtgg aacccttgga tgctgtgaaa gagcatcgag gggtagtcga
    12481 tatgccctaa cgaggataat cgtttcgcat gcgcttctgg aagcgtcaag gatccggcga
    12541 aaaatgacac tagacgtggg gacctgggtt tgtaacagtg cgtttggtgc gttttcggta
    12601 caaagagcgg taatcatggg cgaactcacg ctatcggaca tcaagcaatt gctcatcgcc
    12661 catgggatga ttgtgtatcg aatgacgggc aattccgtgg cgatcgctga gcgtgttcga
    12721 gacaatctga tcatggaagc tcatgtccag gtggttgtcg aatctgggat gagggtgcga
    12781 tttacgaccc gggcccagcg ggcggacttt ccatcgtcgt ctgaaacgga accaaccctg
    12841 ctagcgcgcg ctcgcgaatt gggtaagcca gcgttatcat taggtttttc agagatatgc
    12901 accacggtgg aatccatctc cgatccgatg gatgcctctt gttcgttgga tacgtggtac
    12961 caggtgcatt tcgaaaagct cgtgaatacc gacgaagagc tgatttgcgc cgtccagcaa
    13021 gcgatggtcg ttaaaaaagt cgcgcctcgt tgaccagccc tatcaacgac gcatggacct
    13081 ttgttgggtc gcgtggtatg cgccctgccc tacaccatcc aagagacatc agttcgaatg
    13141 ttaggcggcg gcggcacaca aaaaggtttc atgtcgttcg tgtacggggg aatcgtactc
    13201 gcgatcgcca tggtcttcat cgttcaattt cgtccaggct ccggtcgaga gacggcttcc
    13261 ttgacgcgac aatgcgcggt aaccattcga gaccgatgca tcgagcccaa agagtttctc
    13321 gcgagtctgg gtttggctgc gccacggggt gctgatgatg accgtttgcg cgccatgcaa
    13381 atccgaaggc aggtcgccga aggtctcatc gagcggacct tgctcgtgca agatgccaag
    13441 cgtctcgcca tgactgtctc cgatgacgaa atcaatgcgg aact
//
LOCUS       DAOSOR010000089        13317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4679_length_13317_cov_4.519529, whole genome shotgun sequence.
ACCESSION   DAOSOR010000089 DAOSOR010000000
VERSION     DAOSOR010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 13317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13317
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4679_length_13317_cov_4.519529"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PK710_06890"
     CDS             complement(<1..182)
                     /locus_tag="PK710_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09476.1"
                     /translation="MLRNALRRSVWFVPALLLASFLFFWLLTLVAPTPDPSLPLFFIL
                     YPRDVKILTDRAVRSLA"
     gene            complement(201..1358)
                     /locus_tag="PK710_06895"
     CDS             complement(201..1358)
                     /locus_tag="PK710_06895"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOT09477.1"
                     /translation="MTEASPSQPGDIRFHVFGRTDVGRIREHNEDSYIIANLTEKTCG
                     TEDQNHLHVVGPHGSLLAVCDGMGGAAAGEVASALAVSIIYEKLAEGAPPTDHDELAR
                     RMVRAIETAGLRIYSEAKLDRHHRGMGTTATVAVMLDSFLFLGQVGDSRAYILRNQHL
                     VQVTSDQSLVNQLIKAGQLTEEEAENFEHNNIILQALGTADTVQVDLTFVPLRQGDVL
                     LMCSDGLSSMVRYDDIRQTMLDHPDITEACQTLIEKANDAGGHDNITVVLARFEGEGL
                     PEPADDDPRLEYTNYVLPENSTSERAMPGRRGAVKELDEPMIGSEVKDRFIDPSQPRA
                     SSSNNFNASPKNLDDEVVLPIENTPLWVLAALVIAVFGGLAFAAYYFVHRQ"
     gene            complement(1382..2722)
                     /locus_tag="PK710_06900"
     CDS             complement(1382..2722)
                     /locus_tag="PK710_06900"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT09478.1"
                     /translation="MICDRCGRDNPTSLIFCQDCGRRLQAVAPPAISRPAPENSESSA
                     KHVVCERCQTRNPAEGKYCVTCGAPLHPSPPIANLSPIPPAGVIQVSPPDPPTASPQP
                     IICDRCQGICDGGMRFCKYCGAPLPNPSFQEGPPQQVAEPSPALNSPSAGAQDYVFQQ
                     QNNISESARAAVAPPTPPPGAPNMRAESTDRSRTRPSTSQGLAQPHPLLADQPAGTKT
                     GQYIPFSGSSTIPAGRLVVVGSDGREGPSFPLVGDQVDIGRSEGAIVLSEDHYMSPRH
                     ARLLRVNGSWFVRDLNSTNGVFVRLRSPHPLQHGDLLLLGVEVLKFETAMDAELDLGP
                     AVQHGTLVFGSPALARCARLVQRTVEGVARDVYHLHRLETVLGRESGDIVFTDDPYLS
                     RRHAVIRRSSSSGTFTLEDLNSSNGSFVAIRGDCALQDGDSIRVGQHLFRVELA"
     gene            complement(2829..3530)
                     /locus_tag="PK710_06905"
     CDS             complement(2829..3530)
                     /locus_tag="PK710_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09479.1"
                     /translation="MIRKRSNVAVLGYVAISATLAVSSALGQQPPQPQPPAPKHPPAG
                     PVQPPPLNVDYLQFGTALHVQFSLNPGPVCPSHARVPCILGSGAGLVARAGYRSRGPW
                     YIGGAYSLAKLDTQGLMRLGVLQQLGGEMRYLLNFGLRTEPYLTAGLGGLAFGNEWHA
                     EAWGLASFGGAGFEVQLSRTTIVGLAATYRPMLFFEWTDRAGQHRDTGVAHFWGLALT
                     LEAREPLGSHGMRLR"
     gene            3658..4020
                     /locus_tag="PK710_06910"
     CDS             3658..4020
                     /locus_tag="PK710_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09480.1"
                     /translation="MEIYLDKRAVRQIRLSAEDATEEGDTETLREDIFESFSEEQIED
                     IERLLDNGDFFEFLTDMLEEWGGDDVDELFEILETSLGELNIDLKYALPAEDDDDDPD
                     PDDDDEDFDPDEDEVDDD"
     gene            complement(4089..5171)
                     /locus_tag="PK710_06915"
     CDS             complement(4089..5171)
                     /locus_tag="PK710_06915"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT09481.1"
                     /translation="MSKVIAYAICACCLLGCAPSIPRPYLEAKAAGHRAYSSGRYEQA
                     AEHFHQAAGKADRRKDRDEALYLEASSYLRAGRYREAKTVFEAMVSISPDGLRAARAA
                     HEAAKIEIRHGDAAKGWQMLHAAIVRYPASGLSRAALQRYLQYLDETIGPLRVIDYLD
                     ASMDWYRAHDLGERALYEKAKRLERLGRLREAVDTLLICAKDYPYPVGSLRDNALYLA
                     SELEERLGRPRQAVAHLRTLLEDREQSLIHGSYERPLYAPAQMRLAVLYRDALAEPDV
                     ARKEFRKLFDAFPTSTLRDDALWAEALVARKQKDTEGACAAVSLLVKHIPQSRYAHCA
                     TLLCDKAPTPGQAGTCREYIARDIAP"
     gene            complement(5174..6232)
                     /locus_tag="PK710_06920"
     CDS             complement(5174..6232)
                     /locus_tag="PK710_06920"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HOT09482.1"
                     /translation="MTTAPSAMIDLRSDTVTQPTPSMRQAMQRACVGDDVFGEDPSVR
                     LLEEEVADRLGTQAALFVPSGTMGNQLAIGVQTRKGDEIIVGQGAHCAWYESGAASAL
                     WGVQAVSVGHDGCFDGEHVSAAVKPRADWYPQTRLVAVENTHNRAGGRVWRLEQLASV
                     LEASRRHGLSCHMDGARIWNAAVALGVTEKRIATGFDTVSVCFSKGLGAPVGSALCAS
                     HALIEQARRLRKRLGGGMRQAGFLAAAARFALQQHRERLVRDHDNAKALARIVASAKG
                     AQVDLEAVQTNIVMVDTPTIAAEQIEVAARAKGVLVAVFGAHRIRLVTHLDVEETAQR
                     GGQIVAEVIETLQRLHGE"
     gene            complement(6380..8086)
                     /locus_tag="PK710_06925"
     CDS             complement(6380..8086)
                     /locus_tag="PK710_06925"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOT09483.1"
                     /translation="MNQRFELSEIRSNCRKLGPWAEYLLGCQPMRHALLNLRTLALLV
                     VGTTIFACGGEEEAAPDPEATVAEEGDGQAPAEDRIETPMPPEDGPKLVATSLATVIY
                     SQPSSNSEKIGYLRVGEVVARSKTAISYDGCEHGWYRIRPVGYVCIGDKASTYLQHPI
                     ARALGKLPDLSKPLPYRYAFARAIAPNYLRVPTKAEQHQYEFQLDKHLRSYNKLKDSW
                     QAIDVGANDIPLDENGVASGPAPSEPPHTDLSTVFGGDGNDMVPWWLDGGRKIPHLAA
                     YKAPQYAVIAGRVKRFTGVAVIGTFVPGEGAQQRRMAITADGRLIPVSKLKPNTGSTW
                     HGVALGQKSGLSLPLGFVTNRSGAAVFELSDTETKRVGEIPFRTTINLNGKVRNRQGK
                     RFVQLHNDTWVLSDDAAIAALPSKLPAFAKGQQKWIDLSIVSQTMVAWEGRTPVYVTM
                     VSTGKDGLGDPKTSHSTPRGTFRIREKHVTTTMDSTDVGHEFELRDVPWVQYFHHGYA
                     IHAAYWHDDYGKVRSHGCVNLSPIDARFFFFWATPTLPTAWHGAYASRDLGEGTIVYI
                     HP"
     gene            complement(8541..10376)
                     /locus_tag="PK710_06930"
     CDS             complement(8541..10376)
                     /locus_tag="PK710_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOT09484.1"
                     /translation="MTDDEQGKAQDAEEQHPPIPSPSPVPSPPDVSMLDAALGPDDVA
                     SYLQAPPPEETAVRDKLLSTPSEEELDPLEDDEPALEQSDETIDQDSTSDPHVPAELR
                     SSRQRITLKIPDDAIPKSSATPAVEEAKPPITEQRAPIVQAMRIIAVGEASAPAPEKQ
                     VQQLALEDRLDDDTEQGFESQPVASSRNDEELEPEWEEDLVRAPPVMIVPTPSVPSDS
                     PAPPMATPLSPSASAPPPPIKTAARAGAVVLPVPQTSQELSESPPSKELTPAASQDAP
                     ADELDEELAPVSDPSVSPTPPTAATSNEIPEERPLGVTPPVAVSVPLPAAPAPSAEPA
                     NDIPPAPIRPAKAPPPPKVKPQEREKRPRKGRAGWEEAFTDEFVRAYPRLTPGQVKAE
                     VSFIEESLGIQYGGVVLDLGCGSGQHAVELASRGFNVVGYDLSLTMLAMAADEAQERG
                     QKINFLQGDMREMAFEEVFDGVFSWATSFGFFDDEINASVIQRVHRALRKGGMFLLDL
                     INRDYICSRLPSLVWFEGDGCVCIDDAQFNSITSRLRVKRTIMIEDGTSKEVEYSIRL
                     YSLHEIGRILHDTGFKVVEVSGHPATHGAFFDADSPRVITLAVRR"
     gene            complement(10373..11212)
                     /gene="asd"
                     /locus_tag="PK710_06935"
     CDS             complement(10373..11212)
                     /gene="asd"
                     /locus_tag="PK710_06935"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HOT09485.1"
                     /translation="MPVSVPRFLASRIVRRLPRQQISRLMGQVCDRPLSPRVSRSIVG
                     LYRRIYDVDMSDVLPRATPYESFDTFFTRALIPGRRPISDGPGQVVSPADGMLQSMGR
                     IEAGCRIVVKGRPYDVARLIGDETEALSLLGGQYAVVYLSPRDYHRLHAPATGKVSLI
                     RGICGDLFPVNALGDHCTRALLVENKRVVLRIETPDMGWIVLVLVGAFIVGRISVAML
                     SYRDVPEGIHVLSPPVDITRGDEVGAFHLGSTAVVLADPQAPCWQRPLGLIRVGESLV
                     RFG"
     gene            11331..11480
                     /locus_tag="PK710_06940"
     CDS             11331..11480
                     /locus_tag="PK710_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09486.1"
                     /translation="MRENAVSLAIDGVVVPKQGVRLNNAVGLEALGPANGRSFRRFLA
                     EERKI"
     gene            11610..12443
                     /gene="folD"
                     /locus_tag="PK710_06945"
     CDS             11610..12443
                     /gene="folD"
                     /locus_tag="PK710_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOT09487.1"
                     /translation="MQGRILDGKSIAEKVRSEVQQQVEAFRLTSGRAPSLHVVLVGDD
                     PASQVYVRNKNKAAKKTGIDAVTHELPSDTPQAQVEAVVRNLNEDAAVDGILVQLPLP
                     KGLNEQAVLDLIDPKKDVDGLHAVNAGLLSAGRPALVPCTPQGCMRLLAETGVSLQGL
                     DAVVVGRSVLVQQHATVTVAHSRTRDLDALCRRADVLVAAVGRPRMIQGSWVKPGAIV
                     IDVGINRLGDGRLVGDVDFEGAMSRARAITPVPGGVGPMTIAYLLQNTVLAAKGRRVM
                     P"
     gene            12493..>13317
                     /locus_tag="PK710_06950"
     CDS             12493..>13317
                     /locus_tag="PK710_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT09488.1"
                     /translation="MSPVSEAGLTCLREMRRNLRSAKGIVMGLLFLLGGAAASLIYAA
                     VSELTNPNRQGHDVPEEAFRDLRQKVWAEVWNADIGGYLADSPSIFVALFKGSLWFIP
                     FMTLMIGFEQVCGDLQHRTIRYTAIRARRESIVAGKFLAIWGVVAVLLFALHAFVWFV
                     TLIRADASFSQVLVWGPRMWGLSAIYTAAYAGLTILVSSLTRRPVISLFLGLIFFSLF
                     WVLDLAADVARMAESERFSWLQYVGYAIPDYYEAWLVTPKAPQMLGAMAILLAYGVG"
BASE COUNT         2710 a         3782 c         3981 g         2844 t
ORIGIN      
        1 gccaacgaac ggaccgcgcg atcggtgagt attttgacgt cccggggata gagaatgaaa
       61 aacagtggca gggagggatc gggcgtgggg gcgacgaggg taagcagcca gaaaaacagg
      121 aaagaggcga gcaacagtgc gggaacgaac catacgcttc gtcggagggc gttgcgcagc
      181 atcgagaaca gaactccgtc ctattgtcgg tggacgaagt agtaggcagc gaacgccagc
      241 cctccgaaaa cagcgatgac cagagcggcg agcacccaca ggggcgtgtt ttcgatggga
      301 agaaccacct catcgtcgag gtttttgggc gaggcgttga agttattgga agacgatgct
      361 cgcggttggc taggatcgat gaaccggtct ttgacctcgg agccgatcat cggttcatca
      421 agctctttga cagctccccg tcgtccgggc atggcacgct cggaagtcga attttcaggg
      481 agcacgtagt tggtatactc cagccgagga tcgtcatcgg ccggctccgg caggccttct
      541 ccctcgaagc gagccaagac gacggtgatg ttgtcatggc cgccagcgtc gttggctttt
      601 tcgatcagcg tttggcaagc ttcggtgata tcggggtgat cgagcatcgt ttgtcggatg
      661 tcgtcgtacc gaaccatgct gctgagcccg tcggagcaca tgagcagaac atcgccttgc
      721 cgcaggggga cgaaggtgag gtcgacttga acggtgtctg cggtgccgag ggcttggagg
      781 atgatattgt tgtgttcgaa gttttcggct tcttcttcgg tgagctggcc ggctttgatg
      841 agttgattga cgagggattg gtcgctggtg acttggacga gatgctgatt tcgtaagatg
      901 taggctcgac tatccccgac ttgtccgagg aaaagaaagg agtcgagcat gacggcgacg
      961 gtggcggtgg tgcccatgcc tcgatggtgt cggtcgagtt tggcttcgct gtagatgcga
     1021 aggccggcgg tttcgatggc gcgcaccatt cgtctggcga gttcgtcgtg atcggtgggg
     1081 ggggcccctt cggcgagttt ttcatagatg atgctgacgg cgagggcgct ggcgacctct
     1141 ccggcggcgg ctccgcccat gccgtcgcag acggccaaca aggagccgtg gggaccgacg
     1201 acatgcagat ggttttggtc ttcggtgccg cacgtttttt cggtgaggtt agcgatgata
     1261 tagctgtcct cgttgtgctc gcggatgcgt ccgacatcgg tacgaccgaa gacatggaaa
     1321 cggatgtctc caggctggga tggagaagcc tcagtcattt gaccttcgag ttcagtcacc
     1381 cttatgcgag ctccacgcgg aacaagtgct gccccacgcg gatggaatcg ccatcctgca
     1441 gggcgcaatc accgcgaatc gcgacgaacg agccgttcga cgagttcaaa tcttcgagtg
     1501 tgaaggtgcc agaagagctg gaccgtcgaa tgacagcgtg tcttctggat aagtaaggat
     1561 cgtctgtaaa cacgatatca cctgactctc gacccagaac ggtctcgagt cggtgtagat
     1621 ggtacacgtc tctggcgact ccctccaccg ttcgttgcac aagtcgggca caacgagcga
     1681 gtgctgggga accgaacacg agcgttccat gctgtacggc aggcccgagg tcgagttccg
     1741 catccatggc ggtttcaaac tttagcactt ccaccccgag taagagcaag tctccgtgct
     1801 ggagcgggtg aggggagcgg agtcggacga atacgccgtt ggtcgagttc aagtcgcgaa
     1861 cgaaccacga gccgttcacg cgaagcagtc gggcatgtct cggggacata tagtgatctt
     1921 cggataggac gatggcccct tcggagcgtc cgatgtcgac ttggtcgccg accaatggga
     1981 agctagggcc ttctcgtcca tcgcttccca cgacgacgag gcggccagcg gggatggtcg
     2041 aagagccgga aaagggaatg tattggccgg ttttggttcc ggcgggttga tcggccagca
     2101 gcggatgggg ttgtgccagg ccttgggaag tagaggggcg cgttcgcgag cggtcggtcg
     2161 attcggcgcg catgttcggc gcgcctgggg gaggagttgg tggggcaacc gcggctcgcg
     2221 cggattccga aatgttgttt tgttgttgaa aaacgtagtc ttgcgcacca gccgacggtg
     2281 agtttagcgc gggagaaggt tcggcaactt gttgtggggg gccttcttga aaggatgggt
     2341 tgggtaaagg agccccacaa tacttgcaaa agcgcatacc cccatcacat attccttgac
     2401 aacggtcgca gatgatgggc tggggtgacg ccgtcggagg atcggggggg gagacttgga
     2461 tgacgccagc agggggaatc ggtgagagat tcgcgatggg agggctaggg tgcagcgggg
     2521 cgccgcaggt gacgcagtat ttcccttccg cagggttgcg cgtttgacat cgttcacaga
     2581 cgacgtgttt tgcggaagat tcgctgtttt cgggcgcagg gcgagagatc gcggggggcg
     2641 caacggcctg aagccgtcgg ccgcaatctt ggcagaagat gagggatgtc ggattgtccc
     2701 gtccacagcg gtcgcaaatc acggttcgaa aggggacccc gcaacgtgct cggagtcaag
     2761 accccatttc gatcggatgg ccttttttgc cgttttgtat gcttgaaccc ccgcgtgtcg
     2821 tgaggttctc agcgcaagcg catcccgtgg cttcctaacg gctcccgcgc ctcgagggtg
     2881 agggcaagtc cccagaaatg ggcaactcca gtgtcacgat gttgtccggc gcgatccgtc
     2941 cactcgaaaa acaacatcgg tcgataggtt gcggccagtc cgacgatggt cgttctcgaa
     3001 agctgcactt caaagcccgc accgccgaac gacgcgagcc cccaggcttc cgcatgccat
     3061 tcgttaccaa aggcaagacc cccaagcccc gccgtgaggt atggctcggt tcgcaggccg
     3121 aagttgagca ggtaacgcat ctctccaccg agttgttgca acacccccag acgcatcaac
     3181 ccttgcgtat ccaatttcgc gagagaatag gctccaccga tgtaccaggg acctcgggaa
     3241 cgatagccgg cgcgggctac caaaccggct ccggagccga gaatgcacgg gacacgggca
     3301 tggcttgggc agacaggacc tggattcaag ctaaactgta cgtgaagggc cgtgccgaat
     3361 tgtaaatagt cgacattcaa gggcggaggc tgcaccgggc cggccggtgg atgcttcgga
     3421 gccggtggtt ggggttgcgg tggctgttgg ccaagtgctg aactcacggc gagtgtcgca
     3481 cttatcgcca catagcccag gaccgcaacg ttcgagcgtt tccgaatcat cggttctcct
     3541 cacctggaaa cgtccattgt tttatcatca gaaaacaatt cctagcgcag tcttcttgac
     3601 gccttggcgt tgtatcgcat aaagcccctc aagctctggc tctggaggca agaccgaatg
     3661 gagatctacc tcgataaacg cgccgtgcga cagattcgtc tttctgccga ggatgcgacc
     3721 gaagagggtg acaccgaaac cctgcgtgaa gacattttcg aatccttttc ggaagaacag
     3781 atcgaagata tcgaacgcct gctcgacaac ggcgactttt ttgaattcct cacggacatg
     3841 ctcgaggagt ggggcggcga tgacgtcgac gaactcttcg agattctcga aacgagtctt
     3901 ggtgaactca acatcgacct gaaatacgcc ttacccgcgg aagatgatga cgacgatccg
     3961 gatcccgacg atgacgatga ggatttcgat ccggatgaag acgaagtcga cgacgattga
     4021 agtcgagcga ttatccgtgt tgggatagtc gctttcacca cggaagatgc gctctcgacc
     4081 gtagggggtc agggagcgat gtctcgcgca atatattccc ggcatgttcc ggcctggcca
     4141 ggtgtcggag ccttgtcgca tagtagcgtt gcgcagtgcg catagcggga ttgcggtatg
     4201 tgcttgacga gcagcgaaac cgctgcacaa gcgccttcgg tatccttctg ctttctggcc
     4261 accaaagcct ccgcccacaa agcatcgtcc cgtagcgtcg aggtcgggaa agcgtcgaat
     4321 agcttgcgga actcttttcg cgccacatca ggctccgcca aggcatcgcg gtacaacacg
     4381 gcaagacgca tctgcgctgg cgcatagagc ggcctttcgt aactgccgtg gatgagcgac
     4441 tgctcccggt cttcgagcaa cgtgcgcaag tgagctaccg cctggcgagg acgtccaagc
     4501 cgctcttcga gttccgaagc caaatagagc gcgttgtccc ttaaactacc aaccggatac
     4561 ggatagtcct tcgcacaaat gagcaaggtg tccaccgcct cccgaagccg tcccagccgt
     4621 tccaaccgtt ttgctttttc atataacgcc cgctcaccca gatcatgagc acgataccaa
     4681 tccatgctcg cgtccaaata gtcgatcaca cgcaacgggc cgatcgtttc atccaagtac
     4741 tgaagatagc gctgaagcgc cgcacgactc aaaccactag ccggataccg aacgatcgcc
     4801 gcgtgaagca tctgccagcc cttcgccgca tcgccatgtc gtatctcgat tttcgctgct
     4861 tcatgggctg ctcgcgccgc tcgtaaaccg tcgggcgaga tcgaaaccat cgcctcgaaa
     4921 accgtcttcg cctcccgata tcgtcccgca cgaagatacg atgacgcttc caaatacaat
     4981 gcctcgtcgc gatcctttcg acgatccgcc ttgcctgccg cttgatggaa gtgctccgca
     5041 gcttgctcat accggcccga agagtacgcc cggtgtcccg ccgctttggc ctccaggtag
     5101 ggccgcggta tcgatggcgc gcaacccaat aaacaacagg cgcagatcgc gtaggcgatt
     5161 accttgctca taatcattct ccgtggagac gttgtaacgt ttcgatcact tccgccacaa
     5221 tctgtcctcc acgctgtgcc gtttcctcga cgtcgaggtg ggtaacgagc cggatacgat
     5281 gcgcaccgaa gaccgcgacg agcacacctt tggctcgcgc ggcgacctct atttgctcgg
     5341 cggcaatcgt gggcgtatcc accatgacga tgttggtttg caccgcttcg agatcaacct
     5401 gcgcgccttt tgcactcgcc acgatccgtg ccagagcctt cgcattgtca tggtctcgca
     5461 cgagtcgctc gcggtgttgc tgcaatgcaa atcgcgccgc cgcggcaaga aaacccgctt
     5521 gccgcatccc tccacccaac cgcttgcgca accgacgagc ctgctcgatc aacgcatgag
     5581 atgcgcaaag agcagagccg acgggtgcgc ctaatccttt tgaaaaacaa acacttacgg
     5641 tatcaaagcc cgtcgcaatt cgcttttccg ttacgccgag cgccaccgcc gcattccata
     5701 tcctcgcccc atccatatga cacgacaggc catgacgacg cgacgcctcg agcaccgaag
     5761 ccagttgctc caaccgccat actcgtcccc ccgctcgatt atgcgtgttc tccaccgcaa
     5821 ccaatcgcgt ttgcggatac caatccgctc gtggcttcac cgccgcgctc acatgctccc
     5881 catcgaaaca tccgtcgtgc ccaaccgata ccgcttgtac accccataac gcgctggccg
     5941 ctcccgattc ataccacgca caatgggcgc cttgcccgac gatgatctca tcgcccttgc
     6001 gggtttggac gccaatggcg agttgattgc ccatggtccc actcggaacg aaaagcgccg
     6061 cttgtgtgcc caatcgatcc gcgacctctt cttccagcaa tcgcaccgaa gggtcctccc
     6121 cgaaaacgtc gtcccccacg caagctcgtt gcatcgcttg acgcatcgac ggcgtcggtt
     6181 gtgtgaccgt gtcggaacgc aagtcgatca tcgcgcttgg tgctgtcgtc atgccagctc
     6241 tccaccttgc ccgcgactcg tgatgagccg aggtttcacg aaccaaagag catagcagcc
     6301 tctcgccgac agatcgcgaa agactggaaa tccaccgcgt ttttgtcgat tttcgtagtg
     6361 ccgatcagcc ccctgccgcc tacggatgga tgtagacgat cgttccttcg cccaggtccc
     6421 tcgacgcata agcgccatgc catgccgtcg gcaatgtggg cgtcgcccaa aagaagaaaa
     6481 atcgcgcatc gatcggcgat aaattcacgc agccatggct tcgcaccttg ccataatcat
     6541 catgccaata cgcggcgtga atcgcgtaac catggtgaaa atactgcacc caaggcacat
     6601 ccctcaactc aaactcatgc cccacatccg tcgagtccat ggtcgtagtg acatgctttt
     6661 cgcgaattcg aaatgttcct cgcggagtcg aatgcgacgt tttcgggtcc cccaagccgt
     6721 ctttccccgt ggataccatc gtgacatata cgggcgtacg cccctcccag gccaccatcg
     6781 tctgactcac gatgctcaag tcgatccact tctgctggcc tttggcaaac gcgggaagct
     6841 tggacggcag tgcggcaatg gccgcgtcat cggacaatac ccacgtgtcg ttgtggagtt
     6901 gaacgaagcg ctttccttgg cgatttctta ctttcccatt gaggttgatg gtggtgcgga
     6961 aagggatctc ccccacacgc ttggtttcgg tatccgatag ttcgaatacc gcggctccac
     7021 tgcgattggt cacgaaacca agaggcaaac tcagtcctga tttctgtccc aaggccacgc
     7081 catgccacgt ggaaccggta ttgggtttca acttgctcac gggaatgagg cgaccatccg
     7141 cggtaatcgc catgcgacgc tgctgcgcac cctctcccgg cacgaacgtg ccgataacgg
     7201 cgacccccgt gaatcgtttg actcgtccgg cgatgaccgc atattgcggt gctttataag
     7261 cggcaagatg cggtattttt cttccaccat cgagccacca gggcaccatg tcgttgccat
     7321 ctccaccgaa taccgtggac agatccgtat gaggcggttc gctgggtgct ggaccgcttg
     7381 ccacaccgtt ttcatccaac ggaatgtcgt tggctcctac gtcgatggcc tgccaagaat
     7441 ctttgagctt gttgtaggag cgtagatgct tgtcgagttg gaattcgtat tgatgctgct
     7501 cggccttggt cggaacgcga agatagttgg gagcgatggc tcgtgcgaag gcataccgat
     7561 acggcaaggg tttgcttaaa tccggaagct taccgagcgc acgcgcaatc ggatgctgta
     7621 agtaggtgga tgccttgtct ccgatgcata catatcccac cggtcgaatc cgataccaac
     7681 cgtgttcgca accatcgtaa gaaattgcgg ttttgcttcg cgcgaccacc tcaccgacac
     7741 ggagatatcc aattttttct gagttcgagg agggttggga atagatgacg gtggcaaggg
     7801 aggtggccac gagtttcggc ccatcctcgg gcggcatcgg ggtttcgata cgatcttcgg
     7861 cgggcgcctg gccatccccc tcttcggcta cggtggcctc tgggtccggt gcggcctctt
     7921 cttctcctcc acaagcgaaa atcgtggttc ccactacaag cagggcgagt gtccgtaggt
     7981 tgagcagcgc gtgtctcatc ggttggcaac caagcagata ttcagcccaa gggcccagtt
     8041 ttctgcaatt cgatcgaatc tcggacagct cgaagcgctg gttcatccgt tggcatcgtg
     8101 gtatgccctc agccgtgcgg aagctttcgt ttcttgctct cctcctcacg ataggctgcg
     8161 ctggatcggc gtcatcaaca gccggttccc acgctacgcc cgcgggcgtt tcggattcct
     8221 catccgattc ccatcgttca tccgggcctt cccttccttc ttgcgaagat ggatcctgtt
     8281 ttgcttgcgg tgataccgtg tgtttgcgcg ggtactactg cgagaagata ggagcgacga
     8341 gcggttgtgc gtggaatgga ccgtgtgcga aacgggccaa ttgcgcctgt ctcgcgtcgg
     8401 tcgcaggtgc cgattcgtcc tgctcctgtg aagaacggga tgggcatgcc ttcgtcacgt
     8461 gctcgccttg aggggaccga atacaaccga ccgcgtgaaa cgatgcactg cacgatgatg
     8521 cgtggtcaat cacctcgcgt tcatcgtcgg accgcaaggg tgatcacgcg aggagaatcc
     8581 gcatcgaaaa aagcgccgtg ggtggcggga tgcccgctga cttccacgac cttgaagcct
     8641 gtgtcgtgca gaattcgacc aatctcgtgc aagctgtaca gccgaatgga atactccacc
     8701 tctttggacg tgccgtcctc gatcatgatg gttcgtttga cacgaagacg gctggtgatc
     8761 gagttgaact gcgcatcgtc gatacacaca caaccatcgc cttcgaacca aacgagagaa
     8821 ggaagacgag agcagatata gtctcggttt atcaggtcga gtaagaacat tccgcctttg
     8881 cgtagggcgc gatgcacgcg ttgaatcacg gaagcgttga tttcgtcatc gaagaagccg
     8941 aagctggtcg cccacgaaaa gacgccgtcg aatacctctt cgaaagccat ttctctcatg
     9001 tcgccttgca ggaagttgat cttctggccg cgttcttgcg cttcatccgc ggccatggcc
     9061 aacatggtga gagataagtc gtagcccacg acgttgaatc cgcgagaggc caattccacg
     9121 gcgtgttgtc cggaaccgca accaaggtcc aacaccacgc caccgtactg gattcccaag
     9181 ctctcttcga tgaaggacac ttctgctttt acttgcccgg gagtcaagcg cgggtaagca
     9241 cgaacgaatt cgtcggtaaa tgcctcctcc catcccgcgc gtccttttcg aggacgtttt
     9301 tctcgttctt gcggtttgac cttgggaggt ggaggtgcct tggcaggacg aatgggtgcg
     9361 gggggaatat cgttggccgg ctcggcggaa ggggctggcg cggcgggcaa tggtaccgac
     9421 accgcaacag ggggcgtgac acctaggggt cgttcttcgg gaatttcgtt ggacgtagcg
     9481 gccgtgggtg gtgtgggcga aacggatggg tccgagactg gtgccaactc ctcgtccaat
     9541 tcatcagcag gtgcgtcttg agatgcggct ggagtgagtt ctttcgaagg tggtgactcc
     9601 gacaactcct gagaggtctg tggaacgggc agcacgacgg caccggctcg cgccgcggtt
     9661 ttgataggcg gcggaggggc cgaagcggaa ggcgacaacg gcgtggccat gggtggcgct
     9721 ggcgagtcgg aaggcacgct gggcgtcggc acgatcatga ccggtggtgc ccgaaccaag
     9781 tcttcttccc attccggttc caattcctcg tcattccgcg atgatgcaac aggctgcgat
     9841 tcgaaccctt gctcagtgtc gtcgtcgagc cgatcctcga gcgcaagctg ttggacctgt
     9901 ttttcaggag caggagccga ggcttccccc accgcgatga tccgcatggc ttgcacgatc
     9961 ggcgctcgct gttcggttat cggcggtttt gcttcttcca ccgcgggagt tgcagaggat
    10021 ttaggaatgg cgtcgtccgg tattttcagg gtaatgcgct gccgtgacga tcgaagctcc
    10081 gcggggacat gcgggtcgga cgtactgtcc tggtcgatag tctcgtctga ttgttccagc
    10141 gcgggttcgt cgtcttcgag ggggtccaat tcctcctcgc tgggcgttga caatagtttg
    10201 tcacggacag ccgtttcctc tggaggaggc gcttgcagat agctagccac atcgtcagga
    10261 cccagcgccg catcgagcat ggatacgtcg ggaggagagg gaacgggtga tggcgacgga
    10321 atcggcggat gttgctcctc ggcatcttgg gccttgcctt gttcgtcgtc cgtcatccga
    10381 acctcacaag cgattctcca actcgtatca atcctaacgg tcgctgccaa caaggtgctt
    10441 gcggatcagc caacacgacc gccgtggaac cgaggtggaa cgctccgact tcgtcgcctc
    10501 gagtgatatc gaccggagga gacaaaacat ggattccctc gggtacatct cgataagaga
    10561 gcatggccac ggaaattcga cccacgatga aagctcccac gagcaccaac acaatccaac
    10621 ccatatcggg cgtttcaata cgaagaacga cgcgcttgtt ttccaccaac aaggcccgcg
    10681 tgcaatgatc tcccagcgca ttgacgggaa ataaatcacc acaaatcccg cgaatgaggc
    10741 ttaccttgcc cgtcgccggc gcgtgaagtc gatgatagtc ccgaggggaa agatagacga
    10801 ccgcgtattg gccgccaagc agcgataacg cttccgtctc atcaccaatc agacgggcta
    10861 catcataggg ccgccctttg acgacaatcc ggcaaccggc ctcgatccga cccatcgact
    10921 gaagcatccc atccgcaggg ctcactacct gcccgggccc atcgctgatc ggtcgtcgtc
    10981 ccggtatcaa agcccgcgta aaaaacgtgt caaaactctc gtagggtgtg gctcggggca
    11041 aaacatccga catgtccacg tcgtaaatcc gtcggtataa ccccacgatc gagcgggaga
    11101 ctcgcggcga caagggcctg tcgcacacct gtcccatcag acgacttatc tgctgacgag
    11161 gaagtcttcg gacgatccgc gaagcaagaa aacgaggaac ggaaacaggc atggtgtaga
    11221 ttgcgatccg agctgacttt gcatcctcgc agcccggata ggcaatggtc aatatcttgg
    11281 acaaccttgt accatcccgg tcgggggcca aagcccaagg gctcgctagg atgcgggaaa
    11341 atgccgtgtc gcttgccatc gatggcgtcg tcgtgcccaa gcaaggcgtg cgcttgaaca
    11401 atgccgtggg attggaagcg cttgggcccg caaacgggcg cagcttccgc cgctttcttg
    11461 cggaagagag aaaaatataa ttaaatccaa tagttggtga atagctacca ggctggctgc
    11521 cttacgttcc gaccccctta gccttggttg gcttccggtt cacccttcac ccgtgccagc
    11581 gcacctgcta cgggctgatg cccatggaaa tgcaaggccg aatcttggat ggcaagtcga
    11641 tcgccgagaa ggtgaggagc gaggtgcagc agcaggtgga ggccttccgc ctcacgagcg
    11701 gtcgcgcccc gtcgttgcat gtcgtgctcg tgggagatga ccccgcctct caggtctacg
    11761 ttcgcaacaa gaacaaggca gcgaaaaaaa ccggcatcga tgcggttacc catgagcttc
    11821 cgtcggatac gccacaagcg caggtggaag cggtggtgcg taacctcaac gaggatgccg
    11881 cggtcgatgg gatcctggta cagcttcctc ttccgaaagg cttgaacgag caagcggtgc
    11941 tcgacctcat cgaccccaaa aaagatgtcg atgggctcca tgcggtgaac gctggactgc
    12001 tttcggcagg acgccctgcg ctggtgcctt gcacgcccca agggtgcatg cgtctgcttg
    12061 cggagacggg tgtatcgcta caggggttgg atgcggtggt cgtaggacgg agcgtgctcg
    12121 tgcagcaaca tgcgacagtg accgtggcgc atagcagaac ccgtgatctc gacgccctat
    12181 gtcgtcgggc ggacgtgttg gtggccgcgg tggggcggcc gcggatgatt caagggtctt
    12241 gggtaaaacc tggggcgatt gtcatcgatg tgggaatcaa tcggttgggc gatgggagat
    12301 tggtggggga tgtggatttc gaaggagcga tgagtcgggc ccgggcgatc acgcccgttc
    12361 cggggggggt agggccgatg acgatcgcct atttgttgca aaacacggtt ttggcggcca
    12421 aggggcgaag ggtgatgcca tgagtctttt tcgtgacctt tgtgccgggt cgtgtcagcg
    12481 tagcgcccga tcatgagtcc cgtatccgaa gcaggtttga cgtgtttgcg cgagatgcgc
    12541 cggaaccttc ggagcgccaa aggtattgtc atgggcttgc tatttctgct cgggggagca
    12601 gccgcaagct tgatttacgc cgcggtatcc gagctaacga accccaaccg gcaagggcac
    12661 gatgtgcctg aggaagcctt tcgtgaccta cgccaaaaag tctgggccga ggtgtggaac
    12721 gcggacatcg gaggttatct cgccgattcc ccatctattt tcgtggccct gttcaaagga
    12781 agcctctggt tcatcccgtt catgaccctg atgatcggct tcgaacaggt ttgtggggac
    12841 ttgcagcatc gcacgattcg atacaccgcc attcgtgctc gtcgtgaatc gatcgtggct
    12901 ggaaagttcc tagctatttg gggcgtggtt gcggttttgt tgttcgcgct gcacgcgttc
    12961 gtttggttcg ttaccctcat tcgcgcggat gcaagttttt ctcaagtgtt ggtgtggggt
    13021 ccgcggatgt gggggctgtc agccatctat acggcggctt acgcgggatt gaccattttg
    13081 gtcagttcac tgacccggcg gcccgtcatc agcctttttc tgggcctcat ctttttctcg
    13141 ctcttttggg tgctcgatct cgccgccgac gtggcgcgca tggccgaatc cgaacgcttc
    13201 tcctggcttc aatacgtggg ttacgccatc cccgattatt acgaggcctg gctcgtgact
    13261 ccaaaagctc cgcaaatgtt gggggccatg gcgatcctgc tcgcctatgg tgtaggc
//
LOCUS       DAOSOR010000090        13191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4726_length_13191_cov_4.226705, whole genome shotgun sequence.
ACCESSION   DAOSOR010000090 DAOSOR010000000
VERSION     DAOSOR010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 13191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13191
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4726_length_13191_cov_4.226705"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PK710_06955"
     CDS             <1..193
                     /locus_tag="PK710_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09489.1"
                     /translation="FSVEEGNHKVFPGGKPTLFCSELFKPPSADAKGDNWYASNPHHA
                     DHQFHFDFDMTAPIQYSYK"
     gene            533..1066
                     /locus_tag="PK710_06960"
     CDS             533..1066
                     /locus_tag="PK710_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT09490.1"
                     /translation="MITIDELLHLMRTRRSTRRLSRQPLTPQQEHSLLEAFISAPSSG
                     NSQPWHVRIIRDSPLKQRLRQAALNQSFVEHAAAVFVICADTDRAFKAYHRRGVELYC
                     LQDTAAATQNLLLAAHAMGLAACWVGAFRESSVRDVLSLPESLRPVAIIAVGTAEQQP
                     DTPARRPIHEICPDLGG"
     gene            complement(1067..2446)
                     /locus_tag="PK710_06965"
     CDS             complement(1067..2446)
                     /locus_tag="PK710_06965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOT09491.1"
                     /translation="MEETNVKQELRWAAQPVRQLLHLAWPIAVSMLSVSVMTLTDTMF
                     VGWLGASPLAGVGLGGTAVFVLMCFPWGLLGGAKVLVSQAVGAGRRKEAGPYLGAALL
                     LASVLGLVIMGGGLAAADMLRTLSSTAESGQASVSYFALRALGIPVFLLGVAIRETRY
                     GLGDSRSPMVASVLSNGLNIVLDYLFIFPLGMGVGGAALATVIAQVVETLVLVGVQGK
                     DRLPVLTARWRHIRAVWQLGWPSGLQWAMETGAFSVLAVMISMFDEVQMAAHQIVLQI
                     IHFGFLPAVAVQSAVSVLCGQSVGAGRRQLVKLVARQALMLAITYMGLFAVAMAVFGM
                     VVVRAFTDDVAVQGAAQALIWVAVVFQVFDAANIVARGALQGTGDVRYPAVLGVVLSW
                     VCTPPLMWLLGYRHGMGAAGGWIGLCLEIMVGAVLFWWRLERGGWHHAADRASALAGR
                     HPSPGVASG"
     gene            2745..3026
                     /locus_tag="PK710_06970"
     CDS             2745..3026
                     /locus_tag="PK710_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09492.1"
                     /translation="MSIHRPKRVSFRSDRESESSLDPQEKNRPESWSAAVQALGDNAQ
                     RPYSIKETYSIGQGLTHPKFGLGVVVEVESAKIAVLFESGERRLAHAMT"
     gene            complement(3100..4284)
                     /locus_tag="PK710_06975"
     CDS             complement(3100..4284)
                     /locus_tag="PK710_06975"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT09493.1"
                     /translation="MSAYSTDRSWSQTTWTRRSRQAADRFVLSAVNAAVYFADRSHQP
                     MMGSAPADLVFARGKLRLYRLRPRDAEEYELGTATHRIESPRYRTPVLVVPPLMVRPY
                     IYDLRPEHSMLRTLRDAGLDVFVVDFGVPDRGDRDVRLDDYVLDFMPSFVDAALQASG
                     ASGIAMVGYCMGGLFSLLHVGTFDDSRVRALVTIGSPVNFKKMGVVTIAATLGAPAVD
                     RALDVLGNVPGSLSSVAFKLINGPRTFTRYVDLLRNLHDEDYVRGFLVINHWVNDMIP
                     YPREAFRQMFREIVYENRLLENRLSFGGRTCDFRRIRCPLFAVAGKTDMIATPASTRG
                     IVDLLSSTDKTFREVPGGHVAVVAGSEAPEHVWKPIADWLLDRLVARTMPPAACCQRM
                     KQ"
     gene            complement(4281..4943)
                     /locus_tag="PK710_06980"
     CDS             complement(4281..4943)
                     /locus_tag="PK710_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09494.1"
                     /translation="MRPSEHSVPPSQPNPFRHLGGGNSGGDRPVRVQLAVALVIALVL
                     LAVPLYLWRRPKVDDSASAAASMSSEALAVFRPPPDPSKAVVAAALDGGVETDRVKLG
                     RVWIDSCQRAGVRTPAEQCDRQPYFEEALVKAVLSHDCVPKISQGGSVSYALRIDHQK
                     KTYRVFAGKSGSLRRNQALDALSCVQRSIAQPDWDSLPHQHSKYIIAVLATYPPLDSG
                     KP"
     gene            complement(5090..7414)
                     /locus_tag="PK710_06985"
     CDS             complement(5090..7414)
                     /locus_tag="PK710_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT09495.1"
                     /translation="MSESGSIDEKTEPTRIHRDQPKESRWGAWWARLSRGWMGGALAG
                     AAAVSLDTMWVWSSATEDHESGWLSLFLADWGVVAPALVVTSLAFSLLSILTHPSQPL
                     AFGEAVRWLGSDGTREQVNRAVLLPMGMVGLWAWVVGTAHISRYLLNTLTDARAAGLA
                     MGAVGIMGAMLVVVVAYAVFTGIRAALSSRRREVRARFKAWWTGALAFVLVVAMAVWG
                     IKAGTVGGEGGVLGFMGVFRRLELDLRAPLLLLAVGLGAFLAPSVLRRVWAPLAFAVG
                     LAPLGWTVHAATALDDPQLAVQLERYSPLSQPSLRVLRRLTDRDGDGAAAYFGGGDCD
                     DRNPEIHPLAVDIPGDGIDQDCSGSDAPIPEVVKPMPSDTKVEEQAARLLPKDLNVLL
                     ISVDTLRWDMGFMGYGRNITPAIDALAKKAVVFEKTYAMSSYTGKAIGPMMSGKYPSE
                     THMGWKHYNTYPETDIMVQERLRDAGIWTMAIHCHWYFKKETGLGRGMDVFDLSALPT
                     QGIDATTDTSYSADRLSDAAIKVLSDSANTSKPFYAWVHYFDPHAEYLIHKGTEEFGR
                     KPRDLYDHEVRWTDDQIKRLLDFVAEQPWGEKTAIVVTSDHGEAFGEHGMFRHGFEVW
                     EEIVRVPLIIYVPGVSAKRVTQRRSLVDLVPTILDLMGVKRGEAKHDTDFVSGVSLVP
                     DLLSEPGKEPAQREILVDMPPGPFNEARRAYIRGDMKLIVAGGIRYQLFDLGADPQEK
                     KDLSKDKELLTEQRRHYDQFRAQLREIPLRPPKN"
     gene            complement(7411..8361)
                     /locus_tag="PK710_06990"
     CDS             complement(7411..8361)
                     /locus_tag="PK710_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOT09496.1"
                     /translation="MVVSSLLRDEVESFLVHLDKERRCSPHTVSAYRRDLRQFIEFAE
                     HKLESDAVRLSDVTVYLLRAWLGDLARTRSAATVARKIAAIQTFYRTLMRRRIIMDNP
                     AASLSTPKLRRPLPTLCDVDAAREIVQMPDGSTASLARDRAMLEVLYGSGLRVSELVG
                     LDIHDMDLESATARVLGKGSKERIVPLGEPCVRALRTYLTVRGELRHPTLGQDDKALF
                     LSKRGKRMGVRRVQTLVHQYGALGAGRADMHPHALRHTCATHMLGGGADLRAIQELLG
                     HSSLSTTQRYTHVSIEHLMAVYDRAHPHARGKEKSGRTKS"
     gene            complement(8459..10615)
                     /gene="ppk1"
                     /locus_tag="PK710_06995"
     CDS             complement(8459..10615)
                     /gene="ppk1"
                     /locus_tag="PK710_06995"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOT09497.1"
                     /translation="MVSTAHVEFFTPTEPENHKHKWVPPEHLLNREVSWLHFNRRVLS
                     LAEDETLPLLERFKFLSIVGSNMDEFFMKRIGGLKRWVRLGTETLSPDGLSPKQQLTM
                     CRPIIREIVARQEACYQQQLLPLLSEEGIQILAWEDLDEGDQAWCRQYFIESVYPVLT
                     PLAVDASHPFPFISNLSLSLGIQLTGLHDMPKRFVRIKVPKNRPRWVTLPDSARMLPL
                     EQMIAACLDYMMPGEAFASIHMFRVTRSIVVDTGDYQDDLYQDMEERPGQLRREVADQ
                     LRERRFAPAVRLEVDAAMPEELRHWLMRQLDLGPDDLYERHSPIGLCDLISMGNIDRP
                     DLKYRSFRPRTHPRLSVLRKAPSEEPGTNIFAIIRDNDLLVHHPFHDFDMSVLHFVRA
                     AAEDPKVLAIKQTLYRTTSDSPIVHALVRAAEQGKQVAVLVELQARFDEHRNLQWAQV
                     LESAGAHVSYGVEDLKTHAKLVLVVREEGQGLKRYVHIGTGNYHSETARMYTDIGLFT
                     ADRVLCDDVSHLFNTLTGYSRFDQYRKLLVAPKTMRRRFLELIHREIDFARAGRPARI
                     RAKMNQLEDPKIIGRLYEASQAGVDIDIIVRGFCTLRPGVPGLSERIRVLSVLGRFLE
                     HHRIFIFHNDGNPEYFIGSADWMTRNLRRRVEAVAQVENEGCKRELERIMCTYWETDC
                     NAWEMASDGSYQRRAQGSGPCRSAQDLLMEIETHDE"
     gene            10737..11075
                     /locus_tag="PK710_07000"
     CDS             10737..11075
                     /locus_tag="PK710_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09498.1"
                     /translation="MLDSLLLRKTIQQAIDVFVDRVTNFVPEPTKAENAQPLSPLGKR
                     ILDTLAKHKGGLTANDLAIALDIGLAPLFDHLRELMTSRRLLRKVRGNDQLPLYVLPK
                     SQTTDHQNSL"
     gene            complement(11349..12506)
                     /locus_tag="PK710_07005"
     CDS             complement(11349..12506)
                     /locus_tag="PK710_07005"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOT09499.1"
                     /translation="MKQAKVIATVVLVALAVGCRTESRPQVAGTQPGYGQPGYGQPGY
                     GQPGYGQPGYGQPGYGQPGYGQPGQPGYGQPGAPPATTPPATSPPTGSLPAPPIGSFD
                     ASGTMTASFIRQEAKTVLDALVAALPNDARGRVIGIPLAVIEDPGEVNAFAGCDKTGR
                     AFMGITAPLLTLQAASSEAKAYDELNGTRYHDDYVNSVANSVRGGRAVQGLSPGQLPV
                     PNALDPRKLARQKFLFDEQLAFVLGHELAHHYRGHTGCANGGASTGVSAEDIGRLLSN
                     TVPIFNQPLEVEADVHGVRNTLDAGVRQQGAPWTEEGAMLTLDFFNRLSQFGPEVLLL
                     GFLQTHPHPTFRIPIVQSTAQQWRQSGGQSQPGGTNPFPFPLPFPIPGLGN"
     gene            complement(12607..>13191)
                     /locus_tag="PK710_07010"
     CDS             complement(12607..>13191)
                     /locus_tag="PK710_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOT09500.1"
                     /translation="LPMAAERVCSRCHGTGREVLVPCVECRGEGMVRKKKTMLVQISP
                     GIEHNASQVVARAGSIPHPNKGPGDLELTIKVRPHELFRREGDDVVCSVPISFPQATL
                     GAEIEIPTLAGKGKLRVPSGTQSGTVLRLRGMGIPRRVIGGRGDQLVEVSVEVPVNLS
                     DRAKTLIQELGDEIGAEVQPQRRTFMEKLRDLFG"
BASE COUNT         3142 a         4389 c         3162 g         2498 t
ORIGIN      
        1 gttcagcgtc gaggagggaa atcacaaagt cttccccgga ggaaaaccca cgctattttg
       61 cagcgaactt ttcaaacctc cgagcgccga cgccaagggt gacaactggt acgcgagtaa
      121 cccccaccac gccgaccacc aattccattt cgatttcgac atgaccgccc ctatccagta
      181 ttcctacaag taaagcccta ggatcgcctc tcctccggat caaagaaaat tcttttttaa
      241 tttcaacact tactatcaaa taggtgcgga tcgcctccga caagatagtt catccaacca
      301 ctcgcccttt tcacacacag tgtcctttgc atcgattcga atctgaccat ccctacgcga
      361 gccgccaaag acggaatcgg ctggaccata ctcaaaacgc accaaaagca gattgcccgt
      421 tcgaaggcaa ccatccccac ggctactgcc ctcatcgcta ccgtccgtcc ttttgccgcg
      481 cttgaaaacc ctcaagcttc catcctcgcg ccaaacggtt gatcctgcgg ccatgatcac
      541 catcgatgaa ttgctacacc tgatgcgaac tcgacgcagt acacgacgat tgtcccgtca
      601 gcctctcacc ccacaacagg aacactccct gctcgaagct ttcatttccg cgccttccag
      661 cggcaacagc caaccctggc acgtgcgcat catccgcgac tcccccctca aacaacgcct
      721 tcgacaggcc gcgttgaacc agtccttcgt cgaacacgct gcggccgttt tcgtcatctg
      781 cgccgacacc gaccgcgctt tcaaagccta tcaccgccgg ggtgtcgagt tgtactgtct
      841 ccaggacaca gccgcggcca cacagaattt gttgctcgcc gcgcacgcca tgggacttgc
      901 ggcatgttgg gtcggtgcct ttcgagaatc atccgttcgt gatgtgctca gcctgcccga
      961 gagcctgcgc ccggtcgcca tcatcgcggt aggcaccgcc gaacagcagc ctgacacgcc
     1021 cgcgcgacgt cccatccacg agatttgccc cgaccttgga ggatgatcac cctgaagcca
     1081 cgcccgggga gggatgtcgt ccggccagcg ccgaggccct atcggcggcg tgatgccacc
     1141 cgcctcgttc cagcctccac cagaacaaaa ccgcacccac catgatttcc agacacaacc
     1201 caatccaccc ccccgctgct cccatcccat ggcgataccc caacagccac atcaacggcg
     1261 gagtacatac ccacgaaaga acaaccccca acaccgctgg atatcgaacg tcacccgttc
     1321 cctgaagcgc cccccgcgcc acgatattgg ccgcatcgaa aacttgaaac accaccgcta
     1381 cccatatcaa cgcttgggcc gccccttgta ccgcaacgtc atcggtgaac gcacggacca
     1441 ccaccatgcc aaacacggcc atcgccaccg caaacaatcc catgtacgtt atcgccaaca
     1501 tcagcgcttg ccgcgccacc agcttcacga gctgccttcg ccccgctccc acactctgcc
     1561 cacacaacac ggataccgcg ctttgtacgg ccaccgctgg caaaaatcca aaatgaatga
     1621 tctgcaacac aatctgatgt gccgccatct gcacctcgtc gaacatcgag atcatgacgg
     1681 ccaataccga aaacgctccc gtttccatgg cccactgcag ccccgaaggc cacccgagct
     1741 gccataccgc tcgaatatga cgccagcgcg ccgttaatac aggcaaccga tccttcccct
     1801 gcacgcccac caacaccaac gtctccacca cctgcgctat caccgtcgcc aacgccgccc
     1861 cccctacccc catccccaac ggaaaaataa aaagataatc gagaacgata ttcaacccgt
     1921 tcgacagcac cgacgccacc atgggcgagc gtgaatcacc cagcccatat cgcgtctctc
     1981 gaatcgcaac tcccagtaaa aaaacaggaa ttcccaaagc ccgcagggcg aaatacgaca
     2041 ccgacgcctg cccggactcc gcggtcgacg acaacgtccg aagcatatcg gccgccgcta
     2101 aaccacctcc catgatgaca agccccagca cgctcgccag cagcaatgcc gcacccaaat
     2161 atggccccgc ttccttccgc cgacccgccc ccacggcttg cgataccaat accttcgctc
     2221 cccccaacaa cccccaggga aagcacatca acacaaatac cgccgttccc ccaagcccca
     2281 cccccgcaag cggtgacgcc cccaaccaac ccacgaacat cgtatccgtg agcgtcatca
     2341 cactgaccga gagcatggaa accgcaatag gccaggcaag atgcagcaat tgccgaaccg
     2401 gttgagcggc ccatcgaagt tcttgcttca cattcgtctc ttccatcgga gggacatcaa
     2461 tacgaacaac ggcaggaagc ttgtttacgg gcagaacctc aaacaagcaa ttcagatgct
     2521 cgtagcacgc tacgaatcgc ccccgttgtt tcgccttcct atcctctcct tgcctacagt
     2581 ggcatcctca tgccgcgcac aacccaacgt cataacccca agacacgacg atccctcgct
     2641 ttcaaatcac acatcgaaac gccagatccg cttgtgagcg accaaagctc cgtgtaaaaa
     2701 atattacccc gtccactgga aacccaaacc cccagggtgt ctcgatgtcg atccaccgtc
     2761 ccaaacgcgt cagctttcgc tccgatcgag aatccgaatc cagccttgat ccccaagaaa
     2821 aaaaccgtcc agaaagctgg tcggccgcgg tgcaagccct gggggataac gctcagcgac
     2881 cctattcgat caaggaaacc tactccatcg gacaaggact cacccatccc aaattcggcc
     2941 ttggtgtcgt ggtcgaagtc gaatccgcca agatcgcggt gctattcgag tccggtgaac
     3001 gacgcctcgc gcacgccatg acctgacttg cccacgctcg gaggcggcgt cgatgacaac
     3061 gacgcacgac tcagatcatc ctcccgaagg atgcaaaggt cattgtttca tgcgttgaca
     3121 gcatgctgct ggcggcatgg ttcgagccac caaacgatcg agcaaccaat ccgcgatagg
     3181 tttccacaca tgctcgggcg cctctgaacc cgcaaccacc gcaacatgcc ctccggggac
     3241 ttcgcgaaat gttttgtccg tactgctgag cagatcgaca atcccccggg tagacgctgg
     3301 cgtcgcgatc atatcggttt tgccggcaac cgcgaataga ggacaacgaa tacgacgaaa
     3361 atcgcaagta cgcccaccaa acgacagacg gttttcgaga agacggtttt catacacgat
     3421 ctctcgaaac atttgtcgaa atgcttctct gggatacggg atcatgtcat tgacccagtg
     3481 gttgatgacc agaaatcccc gtacgtaatc ctcatcatgc aggtttcgta gcaagtccac
     3541 gtagcgcgtg aacgtgcgcg ggccattgat gagcttgaat gccacggaag acaaagagcc
     3601 tggcacattg ccaagcacat cgagtgccct gtccacggca ggcgcaccca atgtcgcggc
     3661 aatcgttacc acccccatct tcttgaagtt gaccggcgag ccaatcgtca ccaaagcccg
     3721 cacccgcgaa tcgtcgaacg ttcccacgtg cagcaaagag aacagtccgc ccatacaata
     3781 gcccaccatc gcaatgcccg aagctccgct cgcttgcagg gccgcatcga caaaagatgg
     3841 cataaaatca agcacataat catctagtcg cacgtcgcga tcaccacgat ccggaacacc
     3901 aaagtcgacc acgaaaacgt caagccccgc gtctcgcaac gtgcgaagca tcgaatgctc
     3961 cggacgaagg tcgtagatat acggtcgaac catcagcgga ggaacgacaa gcaccggtgt
     4021 tctgtaccgt ggggattcga tccgatgtgt cgccgtgccc agttcatact cctccgcatc
     4081 gcggggacgg agtcgataca agcgcaattt gccgcgcgcg aaaaccagat cggcgggcgc
     4141 actgcccatc atgggctggt gactccgatc ggcaaagtat acggcggcat tgaccgcgga
     4201 caggacgaaa cggtccgccg cttgccggga gcggcgagtc caggtggttt gcgaccaact
     4261 tcgatccgtt gaatatgcgc tcacggcttt ccagagtcga ggggtggata ggtggcaagg
     4321 accgcaatga tatacttcga atgctgatga ggcagactat cccaatcggg ttgggcaatc
     4381 gaacgctgca cacacgaaag agcatcgagg gcttgatttc gccgaagtga tccgcttttt
     4441 cccgcgaaca cacgatacgt ttttttctga tgatcgatgc gaagcgcgta actcacagaa
     4501 cctccttggc ttatcttggg cacacaatcg tgggaaagca ccgccttgac caaggcctct
     4561 tcaaaatacg gttgccgatc acattgctcc gcgggggttc gaactcccgc acgctggcac
     4621 gagtcgatcc ataccctgcc caacttgacc ctgtccgtct ccactccccc atcgagcgcc
     4681 gccgccacca cggctttgct cggatcgggc ggaggacgaa aaacggcgag cgcttcggat
     4741 gacatagacg ctgccgcgct cgccgaatca tcgaccttgg gtcgacgcca caagtacaac
     4801 ggaaccgcaa gcaacaccaa cgcgatcacc aacgccaccg cgagttgtac ccgcaccggc
     4861 cggtcgcccc ccgaatttcc tcctcccaaa tgacggaacg ggttgggctg tgacggagga
     4921 acggaatgct cggacggtcg catgaatcct cggcgttgta gactcgtttc cacgagcctc
     4981 gcaagcaagt cccgaaggac gctgccaggg tttgagaatc aagcgcaaga taccgaaaaa
     5041 gtcgtagcga aatagaacca tcggcacgac ccaatcattc ccgcaccctt taatttttcg
     5101 gcggccgtag cggaatctcc cgcagttgcg cccgaaattg atcgtagtgc cgtcgctgct
     5161 cggtaagcag ttccttgtct ttcgacagat cctttttctc ctggggatcc gcacccaaat
     5221 caaaaagctg atacctgatt cctcccgcca cgatcagctt catatcgcct cgaatatagg
     5281 cccggcgcgc ttcgttgaat gggcccggag gcatatccac aagaatctcc cgctgcgccg
     5341 gctccttgcc cggctccgaa agcagatccg gcaccagact gaccccagac acaaagtccg
     5401 tatcgtgctt cgcctcccca cgcttcaccc ccatcagatc cagaatcgtg ggaaccaggt
     5461 ccaccaggct ccgacgctgc gtgacacgtt tggccgagac gccaggaacg tagatgatga
     5521 gcggcactcg tacaatctcc tcccaaacct cgaaaccatg ccggaacatc ccgtgctcac
     5581 cgaatgcttc cccatgatcc gacgtaacca caatcgccgt cttttctccc cacggctgct
     5641 cagccacaaa atccaagagc cgcttgatct gatcgtccgt ccaccgcacc tcgtgatcat
     5701 ataaatccct gggctttctc ccgaattcct ccgttccctt gtggatcaag tattccgcat
     5761 ggggatcgaa ataatgcacc cacgcataaa aaggtttcga tgtgttggcc gagtccgaca
     5821 aaaccttgat ggccgcatcc gatagacgat cggctgaata gctcgtatcg gtcgtggcat
     5881 cgatgccctg ggtcggaagc gcggacaggt caaacacatc catgcctcgc ccaagccccg
     5941 tttctttctt gaagtaccaa tggcagtgaa tcgccatggt ccagatcccc gcatccctca
     6001 gacgctcctg caccatgata tccgtttccg gataggtgtt gtagtgcttc caccccatgt
     6061 gggtttcgga agggtacttg ccactcatca tcggaccaat ggccttgccc gtgtacgatg
     6121 acatcgcata cgtcttttcg aagaccaccg cttttttcgc taacgcatcg atggcaggag
     6181 tgatgttccg cccataaccc atgaacccca tgtcccatcg caacgtgtcg accgaaatga
     6241 gcaacacgtt caggtctttc ggcaacaaac gagcagcctg ttcctccacc ttcgtgtcgc
     6301 tcggcatggg cttcaccact tccggaatcg gtgcatccga accggaacag tcctgatcga
     6361 ttccatcacc cggtatgtcc acggcaagag gatgaatctc cgggttgcgg tcatcacaat
     6421 ccccacctcc aaaataagca gccgccccat ccccatcccg atccgtcaat cgacgaagaa
     6481 cgcgcaaaga aggctgagac aacggagaat accgttcgag ttgaaccgcg agttgtggat
     6541 catccaaggc agtcgccgca tgaaccgtcc agcccaaagg agccaaacca accgcaaagg
     6601 ccaagggtgc ccacacacgg cgcaacacgc ttggggccag aaatgctccc aatcccacgg
     6661 ccagcaaaag caacggcgct cgcaaatcca attcgagacg ccgaaataca cccatgaacc
     6721 ccaatactcc cccttctcct cccaccgttc ccgccttgat tccccacacc gccatcgcaa
     6781 cgaccaagac aaacgccaac gctcccgtcc accacgcttt gaaacgagcc cgtacctcac
     6841 gtcggcgtga cgatagcgcg gcccgtattc cggtgaaaac cgcataagct acgacaacga
     6901 caagcatggc ccccatgatg cccacggcgc ccatggcaag ccccgccgcc cttgcgtccg
     6961 tgagcgtatt gagcaaatag cgagaaatat gggctgttcc aaccacccag gcccatagcc
     7021 ctaccatgcc catgggcaat agaaccgccc ggttgacttg ctcacgcgtt ccatcgcttc
     7081 caagccaacg cacggcctcc ccaaatgcga gcggttgaga cggatgagtc agaatcgata
     7141 acaacgaaaa cgccaaagag gtcaccacca gcgctggcgc aaccaccccc caatccgcca
     7201 gaaacaacga tagccacccc gactcatgat cttcggtcgc ggaagaccaa acccacatcg
     7261 tgtccaacga caccgcagcc gcccccgcta gcgcaccacc catccaccct cgcgacagac
     7321 gcgcccacca cgcgccccat cgagactcct tcggctggtc acgatgaata cgagttggct
     7381 ctgttttctc gtcaatggaa ccgctttcgc tcacgacttg gttctcccgg acttttcctt
     7441 gccccgcgcg tgggggtgcg ctcgatcata cacagccatc agatgttcga tggacacgtg
     7501 cgtataacgc tgcgtcgtag acaaactgct gtgtcccaac aactcctgaa tggctcgaag
     7561 atcagctccc ccgcccagca tgtgtgtggc acaggtatgc cgaagcgcat gcggatgcat
     7621 gtccgccctt cccgcaccca atgcgccgta ttgatgcacc aatgtttgta cccgacgcac
     7681 ccccattctc tttcccctct tcgacaaaaa cagcgctttg tcgtcttgtc ccaaggtcgg
     7741 atgccgaagt tcccctcgca ccgtcaagta cgtgcgtagc gcccgaacac acggttcgcc
     7801 caaaggcacg atccgctcct tcgacccctt gcccaacacc cgcgccgtag ccgattccaa
     7861 gtccatgtcg tggatgtcca atcccacgag ttcagacacc cgcagtcccg acccatacaa
     7921 cacttccaac atcgcccgat cacgagccaa cgacgccgtc gagccgtcgg gcatctgcac
     7981 gatctctcgt gccgcatcca catcacataa ggttggcaaa ggacgccgaa gcttgggcgt
     8041 cgacaagctg gccgcgggat tatccatgat gatgcgacga cgcatcaacg ttcgataaaa
     8101 cgtttgaata gcggcaattt ttcgcgctac ggtcgccgcc gaacgcgttc tcgccaagtc
     8161 cccaagccac gcgcgaagca gatataccgt cacatccgac agccgaaccg catcgctttc
     8221 caacttgtgc tctgcaaatt caatgaattg ccgaaggtcc cgtcgatacg cggacaccgt
     8281 atgcggtgaa cagcgacgct ccttgtcgag atgaaccaaa aagctctcta cctcgtcccg
     8341 taataacgat gaaaccacca tgaatggatc tggattagca aaggaacgag acagaaagca
     8401 aaaaacccac gattgattcg gcgtcgtagc gtctgcacgg ggtcagaaaa cagcctcctt
     8461 actcatcgtg cgtctcgatt tccatcagca aatcctgcgc ggaccgacaa gggccgctgc
     8521 cctgcgcacg acgctgatag ctgccatcgg aagccatctc ccacgcgttg caatccgtct
     8581 cccaatacgt acacatgatg cgctcgagtt ctcgcttaca accttcgttc tccacttgcg
     8641 ccaccgcctc cacacgacga cggagattgc gcgtcatcca atctgcgctt ccaatgaaat
     8701 actcggggtt tccgtcgttg tgaaagatga agatgcgatg atgctctaga aaacggccga
     8761 gcacgctcag gacacggatt cgctcactaa gcccgggaac acctggacga agcgtgcaaa
     8821 aaccacggac gatgatgtcg atgtcgaccc ccgcctggct cgcttcgtac agacggccaa
     8881 tgatcttcgg gtcttcgagt tggttcattt tcgcgcgaat tcgcgctggt cggcccgcgc
     8941 gagcgaaatc gatctcacga tggatgagtt cgaggaaacg ccgacgcatg gtcttgggcg
     9001 ccaccaacag cttgcgatac tgatcgaagc ggctgtatcc cgtcaacgtg ttgaacaaat
     9061 gagagacatc gtcacacaag acccgatcgg cggtaaataa accgatatcc gtatacattc
     9121 gagcggtttc actatggtag ttgcccgtgc cgatatgaac gtagcgcttc aacccctgcc
     9181 cctcttcgcg taccacgagc acgagcttgg cgtgggtctt caagtcctcc acgccataac
     9241 tcacatgagc cccggcagac tcgagaacct gcgcccactg caagttgcga tgctcatcaa
     9301 aacgagcctg tagctctacc aacaccgcca cctgcttgcc ttgctccgcc gctcgaacca
     9361 acgcatgaac aatcggacta tcactcgtcg tacgatacaa cgtctgcttg atcgcaagca
     9421 ccttgggatc ttccgctgct gcacgtacga agtgaagcac cgacatgtcg aagtcatgaa
     9481 acggatgatg aaccaacagg tcgttgtcgc gaatgatcgc gaaaatattg gtgcccggct
     9541 cttccgatgg cgctttccta agcacggaca atcgaggatg cgtgcgaggc cggaacgacc
     9601 gatacttcaa gtccgggcga tcgatgttgc ccatcgaaat caaatcgcac aacccgatgg
     9661 gtgaatgccg ttcatagaga tcgtcgggac cgagatcgag ctgacgcatc agccaatgac
     9721 gcaattcttc gggcatggct gcatccacct cgagacgaac cgccggagca aatcggcgtt
     9781 ccctgagctg gtccgctacc tctcgccgaa gctgccccgg acgctcctcc atgtcctgat
     9841 acaaatcatc ctgataatcg cccgtatcca ccacgatgct tcgggtcacg cgaaacatgt
     9901 ggatcgatgc aaacgcttcc ccaggcatca tgtagtccag acacgccgct atcatctgct
     9961 cgaggggaag catgcgcgca ctgtccggga gcgtgaccca ccgcggacga tttttgggca
    10021 ccttgatccg aacgaatcgc ttgggcatgt catgcaaacc cgtgagctga atcccaagcg
    10081 acaagctaag gttcgaaatg aaaggaaacg gatggctcgc atccaccgcc agcggcgtaa
    10141 gcaccgggta cacgctttcg atgaagtact gacgacacca ggcctgatcg ccttcgtcta
    10201 ggtcctccca cgcgagaatc tgaatgccct cctcgctcaa taatggaagc aactgctgtt
    10261 ggtaacacgc ctcctgcctc gccacgatct cccgaatgat cggtcgacac atggttagtt
    10321 gctgttttgg gctcagcccg tcgggcgaca gcgtttccgt accaaggcga acccagcgtt
    10381 tgagcccacc gattcgcttc atgaaaaact cgtccatgtt cgaccccacg atggacaaga
    10441 acttgaagcg ctcgagcagc ggaagggtct cgtcctccgc aagcgaaaga actcgacgat
    10501 tgaagtgaag ccaggacact tcacgattga gaagatgctc cgggggaacc cacttatgtt
    10561 tgtggttctc gggctcggta ggcgtaaaaa actcgacgtg ggcggtggaa accatgacga
    10621 aggcctcaaa gaaggatact cggttgggaa aaaatcagcg aatcttctgc tatccaaccc
    10681 atgggatatg cgaataccca ttgccaaagg cattgatggc gtttagggga acaacaatgc
    10741 tcgactctct tttgctgcgt aaaacgattc agcaagccat cgacgttttc gtggatcgtg
    10801 tcacgaattt cgttcctgag ccaaccaagg ccgaaaacgc gcagccccta tccccgctgg
    10861 gaaaacgaat cctcgatacc ctcgcgaaac ataagggcgg cctgaccgcc aacgacctcg
    10921 ccatagccct ggatattgga cttgctcccc ttttcgacca cctgcgggaa ctgatgacct
    10981 cccgacggct cctgcgaaaa gtccgaggaa acgatcagct tcccttgtat gtcctaccga
    11041 aatctcagac cacggatcac caaaattccc tttaaatttg ccgcgttatg caaatcgaaa
    11101 gggtcccctg tcgattcatc atccccatcg aacaccaggc tacttgctgc acgaaatacc
    11161 catagccctc acgtttcgat aatggggctc cctcgttcct agccacgaac cacctttggg
    11221 gttccacagt caaccgctac gcctcgcggc ttcagcgcta cccttttcca gttcgggcac
    11281 cgactccgat tcgagccatc aaggaagaag acgaaacgac cggtccgggg ctctacccca
    11341 atcccacttc aattaccaag gccgggaatg ggaaaaggca aggggaaggg gaaaggattg
    11401 gtccccccag gctgactctg tccaccactc tgacgccact gctgcgccgt agactgaacg
    11461 atcggtattc gaaacgtcgg atgagggtgg gtttgaagaa atcccaacaa cagcacctcc
    11521 ggtccgaatt gcgatagacg attgaaaaag tcgagcgtca acatggcgcc ctcttccgtc
    11581 caaggcgccc cttgctgacg caccccagcg tcgagcgtgt tccgaacacc atgaacatcc
    11641 gcttccactt cgaggggttg gttgaaaatc ggcacggtat tggacagcaa acgaccaata
    11701 tcctccgcac taaccccggt actggcccca ccgttggcac agcccgtatg cccgcgataa
    11761 tgatgcgcca gctcatggcc cagcacaaac gcgagctgct cgtcgaacaa aaacttctgt
    11821 cgcgccaact ttcgcggatc aagagcgttg ggcaccggaa gttgccccgg cgacagcccc
    11881 tgcaccgccc tgcctccccg cacggaattg gccaccgaat tcacataatc atcgtgatat
    11941 cgcgtgccat tcaactcgtc atacgctttg gcctcagagg aagccgcttg taacgtcagc
    12001 aacggtgccg taatccccat gaatgcccga cccgtcttgt cacaacccgc aaacgcattg
    12061 acttccccag gatcctcaat cactgcaaga ggaatcccta tcacacgccc ccgggcgtca
    12121 ttgggcaacg ccgccaccaa cgcatcaagt accgttttcg cctcttgccg tatgaatgac
    12181 gccgtcatcg tccccgaagc atcgaaagaa ccaatcggcg gcgcgggcaa cgatcccgtg
    12241 ggtggagagg tcgcaggtgg agtggttgcg ggaggtgccc ccggttgacc ataacccggc
    12301 tggcccggtt gaccatagcc cggttgacca taacccggtt gaccatagcc cggctgacca
    12361 tagcccggtt gcccataacc cggctgacca tagcccggct gaccatagcc gggctgcgtt
    12421 cccgcaacct gaggacgaga ctcggttcga catcccacgg caagcgcgac cagcaccacg
    12481 gttgcaatca ctttggcttg tttcatcatg ctgtccctcg agggctcaag gaaacgatcc
    12541 acatacccaa aaagacgtac ttgcccacct gctcattcat tgcatcctac ggcccgctgc
    12601 ttgctttcag ccgaatagat ctcgcagctt ttccatgaaa gtgcggcgct gaggctgcac
    12661 ctcggctcca atttcgtctc ccaattcctg gatgagcgtt ttggcacggt ccgataggtt
    12721 gaccggtacc tccacggaga cttccacgag ttgatcccct cttccaccaa tgactcgacg
    12781 gggaatcccc atgccgcgga ggcgaagaac cgtccccgat tgcgttccgg aaggaacccg
    12841 gagctttccc ttgcccgcaa gcgtgggaat ttcaatttcg gctccaaggg tagcctgggg
    12901 aaaggagatg ggcacggaac acaccacatc gtcgccctca cgacgaaata actcatgagg
    12961 ccgaactttg atggtgagtt ccaagtcccc cggccccttg tttgggtgag gaatgctgcc
    13021 cgctcgggcg acgacttggg aggcattgtg ctcgattccc ggcgagatct gcacaagcat
    13081 cgtctttttt ttgcgcacca tgccttcgcc acggcattcg acgcaaggca cgagcacttc
    13141 tcgtccggtc ccatgacacc gtgagcaaac gcgctcggcc gccatgggaa g
//
LOCUS       DAOSOR010000091        13141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4747_length_13141_cov_4.301926, whole genome shotgun sequence.
ACCESSION   DAOSOR010000091 DAOSOR010000000
VERSION     DAOSOR010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 13141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13141
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4747_length_13141_cov_4.301926"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PK710_07015"
     CDS             complement(<1..202)
                     /locus_tag="PK710_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09501.1"
                     /translation="MQASAITFAFGRSLLPFGIALLLGGCEPTPQPPPPPRPSASIAQ
                     QPTAAPAPPEPTVSGAPIALRDV"
     gene            569..976
                     /locus_tag="PK710_07020"
     CDS             569..976
                     /locus_tag="PK710_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOT09502.1"
                     /translation="MTDKKLRILIAKPGLDGHDRGAKVVARALRDAGFEVVYTGLHQT
                     PAMIASAAVQEDVDAVGLSIMSGAHNTLFPAVMEAIAEKGVDDIVVFGGGIIPDEDIA
                     TLRSKGVKGLFTPGTSLQDIVNWVRENVQPRAG"
     gene            complement(986..1627)
                     /gene="rsmG"
                     /locus_tag="PK710_07025"
     CDS             complement(986..1627)
                     /gene="rsmG"
                     /locus_tag="PK710_07025"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOT09503.1"
                     /translation="MNRRPLPPRSDTIPSRLEQLAHALETPLDPTIRLQLVQWIETIL
                     HWNQKIDLTAARTIDELLDLCVADAIVLSRSIEANRRVVDVGSGAGAPGLPLALLRPD
                     LTVTLVEPLAKRVAFLRTVAGSLPTTVSIQRTHGENLPHHGWDVAISRATLEPSAWLA
                     MGLHLVDSKGKVAVLLARQEISPPIDAVSEEDISYRWPLTQAQRRVRWFKRGR"
     gene            1810..2262
                     /gene="mraZ"
                     /locus_tag="PK710_07030"
     CDS             1810..2262
                     /gene="mraZ"
                     /locus_tag="PK710_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOT09504.1"
                     /translation="MAIMFRGQFTHSIDAKGRTSLPSRFRDTMGSGEPRLVLTPALFD
                     PCIHVFPFEAWEAFEARVAEMPQFDPNVVRLRRLYVSAAVECDLDKQGRVLVPVHLRE
                     HAALQREVVWAGVGNKAELWASERWVDATKMTPENWDDLKKAMSEWRL"
     gene            2262..3200
                     /gene="rsmH"
                     /locus_tag="PK710_07035"
     CDS             2262..3200
                     /gene="rsmH"
                     /locus_tag="PK710_07035"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOT09505.1"
                     /translation="MWADVEDSPMEHEDLGYAHTTVLRDETVSLLGLRAGGVYVDATL
                     GGGGHAEAILEASPGVMVVGVDRDPAAMAAACKRLSCFGTRFQGIAGAFADLPTLLSN
                     AGIRKVDGIVADLGLSSAQLADANRGMSFRLDGPLDMRMDPSLEETALDLIRRLSQEN
                     LADLIYRFGEERRSRRVARCVKLALQDERLNTTRDLRRAVVRAVGPGRVGGVDPATRT
                     FQALRIAVNGELDQLAALLERAPTVLTDTGVLAVISFHSLEDRMVKRAFQNPSVWKAL
                     TKGPITASEEEENHNPRARSAKLRAARRVSEHEENP"
     gene            3197..3520
                     /locus_tag="PK710_07040"
     CDS             3197..3520
                     /locus_tag="PK710_07040"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HOT09506.1"
                     /translation="MNRSRPFLILWTLAVFASVAAFVLHLGLRSRTFKLGYEIGRVRN
                     EQAQLKETVHALQLEAESYRNHERVSMIAKTLLGMQPPTRINTMRAVVVPDESKTTTT
                     VHAQR"
     gene            3523..5589
                     /locus_tag="PK710_07045"
     CDS             3523..5589
                     /locus_tag="PK710_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOT09507.1"
                     /translation="MKNLSPRRASGIRLRMAVLCGLMGIGLGVVVSAAYGIQVERGQE
                     WRELAEKQRQRRLHISPRRGTIFDRNGATLADSVEVPSVSVDAFEMFRNVSDQQFPQF
                     LDNASQRLAKALSLDPDQVRDKLARRRRFASLKRRVTVAEVEAIRKLERGEDGGPRLV
                     GLMVEGEGHRYFPNRELAGPMLGFVAPDGEGKDGIELTLNEQLRGHVEEVRGLRDRAG
                     RLIFSEGIKNEKTLAGYNVHLTIDKGIQFAAERELEAAAHTFEAKSGSVVVVNPQTGE
                     ILAMASYPSCNPNDIAQSSLESRRNRAITDQFEPGSTMKVFTMAAALAHKVITPTQPM
                     YCEEGAMAIDNVLVRDTHPSAWLTPTQVLALSSNIGASKLGLGLGDRNLYEALQRFGF
                     GKQTGVPLPGETPGVLRPRDRPWVQVETAAASFGQGISVTTLQMAMATAAIANRGRLL
                     RPILVKRITDGSGAVVEETPTEVLRNAVSANVARVVSEMMVAVTEGEGTGTEAAVPGF
                     RTAGKTATAQKTDPATGKYSADLYTASFVGFVPADNPRIVVAVVLDEPMISHAGGAVA
                     APVFRRVCQASLRVLGVVEEQGPPVAQMEDVVDLAEENYAIMLPKPPIPALNPVVSTS
                     GSDQGAKTVVDVTGLPATSALQTLMKQGFIPQMVGSGKVVRQHPAAGAVVPPGTVVQL
                     YLEPPT"
     gene            5586..7115
                     /locus_tag="PK710_07050"
     CDS             5586..7115
                     /locus_tag="PK710_07050"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOT09508.1"
                     /translation="MTSPLRADGALLSQIAQRLGGTLHGKEDPRITGLHHDSRKIEPG
                     DLFVARQGERSDGSQFITQAVEQGAVAVMTATMPPTEPAVPVLLVDNVRTVLGQAANV
                     IYADPTSRLDVVGITGTNGKTTTSYLVRAAIDGAGGRSGVLGTLGAAFENLHFPASHT
                     TPEADEVARVAFAMKNYGASHLVMEVSSHALDQRRADGVHFRVAAFTNLTQDHLDYHG
                     TLQAYAAAKDRLFFDLDPRAAVVMIDDDHGAELAQRVARRNPNTIRVSTDAKGHADVR
                     PEGAVHFHARGLRCRVRTPQGLFPLDAPYPGKHNLSNLLLTFGILSALDLSIERAAQA
                     LRTSPPVPGRLERCDDDLDDITVLVDYAHTPDALERALRTIRPLTQGRVLCAFGCGGD
                     RDRSKRPLMGDVVGRLADVAVVTNDNPRTENPQSIVDAILQGMTHANAEVIVELDRAL
                     AIDKVVRLAHPHDVVLIAGKGHEQYQIIGEQIHHFDDRQQAREALERRRLGGKDDPWQ
                     R"
     gene            7103..8575
                     /gene="murF"
                     /locus_tag="PK710_07055"
     CDS             7103..8575
                     /gene="murF"
                     /locus_tag="PK710_07055"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOT09509.1"
                     /translation="MATMIPPNRAAFELQQLVAFVQGEWDGSGCTTVEGVSTDTRTLV
                     PGNVFVALVGERFDGHEHVEAAMRAGARAVVVSRPVEVPGGYAVIRVKDTLEALGKLG
                     CAHRQRWARQARANGEDKKIVAVTGSAGKTTTCRATTAVLDAVGNASVHASAGNMNNA
                     VGIPMVLLGLDSSHGQAVVEVGTNHPGEIAYGASLVQPDVAVVTLVACAHTLGLGTID
                     DVANEKAALLAALDSTGVAVVNADDPLVMARVGRTRARRVLTFGKSERAAVRIVGVKS
                     RGWQGQTVVYAVGDGSPNIEFNVDIPLLGDAGSYATAAALAAAWGVWGDAMDWQRAVH
                     GLQALRSESGRLRVVPLRCGAIVIDDAYNANPASIRDSVDVAARMALERGGGLTLVLG
                     EMRELGEHSEREHRRVGEQVGKVRPSAFVAVGGDAWFMAEQARALGANAVFVDDAERA
                     VAAVLERIGGKDVILVKGSRGVALEHVVGALEAWGEGQSA"
     gene            8572..9702
                     /gene="mraY"
                     /locus_tag="PK710_07060"
     CDS             8572..9702
                     /gene="mraY"
                     /locus_tag="PK710_07060"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOT09510.1"
                     /translation="MIYELLYSLRGEASFLTWLNVLRYIPFRVIAATITAMVLAFLLA
                     PWFIRELQRKQIGQVVRQDGPESHLSKAGTPTMGGALILLCLLVPTMLWCDVRNPFVL
                     ATTAVTVGYGMIGYLDDYLKLKRKSSGGLAGKYKLLGQFLIAGAAIGYTFVWTEGLPA
                     DWLEIRTRLSVPFMAFNKYPITLPVAVYVVFAVLVVVAWSNAVNLTDGLDGLAIGPVL
                     INASAYLFLSYVAGAVFFGFSVAKYLDIPPLQSASELAVYCGAMVGAGTGFLWYNTYP
                     AQVFMGDVGSLALGGGLGMLAVFTKNELLSIVLGGVMVLETVSVVTQVVSFKLTGKRV
                     FLMAPIHHHFEKKGWPEPRVIVRFWIISVLLALVSLSSLKLR"
     gene            9615..11063
                     /gene="murD"
                     /locus_tag="PK710_07065"
     CDS             9615..11063
                     /gene="murD"
                     /locus_tag="PK710_07065"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOT09511.1"
                     /translation="MAGATGHRPVLDHFRAARLGEPVVSQTAVKKELKSMDLRDKRVI
                     VVGLGRSGIAAARLCLSRGARVTATDRLERAAVANDILELENQGARLVLGGHEGLVWK
                     DADIVVMSPGVPPFAELFQAERAGVQVIGELELACRFLHAPLGVIGGTNGKSTVTSWL
                     GQMVQDRGKVFVGGNLGTPFSCHVDERWDCVILEVSSFQAERVPTLHPRAAALLNVTD
                     DHLDRYASLEQYAEAKGNVFRNMGPEDVAVVPAGDLICAKQAARGRARIVTFGPGGDV
                     RPEQGEIIHSQKGWRFALSRIALPGQHNVLNACAAIAMAAALGASAESVERGLVSFEG
                     LHHRMQFVAEMQGVSYYNDSKGTNVGASVAALRGLTARRVVLIAGGRDKQGSYEPLVQ
                     VLRERGRAVVLIGEAADRIAEAVADAVPTFRASDMDDAVRIAKQAALSGDAVLLSPAC
                     SSYDMFKDYAHRGEVFRSAVHNLMKSHGKEAP"
     gene            11060..12274
                     /gene="ftsW"
                     /locus_tag="PK710_07070"
     CDS             11060..12274
                     /gene="ftsW"
                     /locus_tag="PK710_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOT09512.1"
                     /translation="MSWLRKRMGAADQTHVGAVDPVLAATIIALVGFGIVMVYSASAI
                     EATVRYHDPEHFLKRQFMFGIAGLGVMGAVSYIDYHRLRRLTYPVLGSVTVLMILTVI
                     GMGVRRGGAARWLTVGPILIQPAEMAKLALVVWLAYSLAKKADKVGSFTVGFIPHVLM
                     AGGLILLCLKQPDFGSAVVIAFLTFALLFVAGARMAYLFGGLIAVIAIGVQLVMSQGY
                     RMARLTAYLDIAKHRQGSAYQPFQSVMSFGSGEITGLGLGKGLQVLYLPEAHNDFIAA
                     IIGEELGFIGVLGLASVFLLIVARGVRAAFRASDDFGSYLAFGISSMLGIQALVNMAV
                     AMAILPTKGLTLPFISYGGSSLLVNAAAAGVLLNISRPRTQRQEVEPARTVPEDAEAS
                     EILMVSAQEGAG"
     gene            12271..>13141
                     /locus_tag="PK710_07075"
     CDS             12271..>13141
                     /locus_tag="PK710_07075"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HOT09513.1"
                     /translation="MSVSLLFAGGGTGGHIFPLLAVAQAIRARRSDTQIVFVGTARGM
                     ESRILGQRGERLELLDILPIKGGGWRGAARGIVRAAESLPAAHRLVSQVQPKAVLSLG
                     GYAAGPVSLAARTKGIPLALLEPNSVLGLANLLLAPFAHRAYLAFPQAKRWFRKSVVR
                     NTGVPLRSGFEPQAYEPTPDQFHILVLGGSQGAVTLNRNVPQAVGLARERTKSVLHVV
                     HQSGHDRTAEVEQTYERAGLRDAVRVVPFIDDVPRALARADLVIQRAGASALAETCAI
                     GRPSLLVPYPFAAG"
BASE COUNT         2541 a         3557 c         4305 g         2738 t
ORIGIN      
        1 cgacatcgcg aagggcgata ggggcacctg ataccgtggg ttcgggcggc gcgggggcag
       61 cggtgggttg ctgagcgatg gaagccgatg gtcgcggggg aggcggaggc tgtggcgtag
      121 gttcgcaacc gcctagcagc aaagcaatcc cgaaaggaag gagactcctg ccgaaagcaa
      181 aggtgatagc cgaggcttgc atgcggtggc atatagcaaa ttcgtggttg ccccgtggca
      241 aattcgagca agaaggcatg aaccatgggc aattgcgttg gctgtctggc ctggaggaaa
      301 gtgaaaaaaa agaggagacg gttcgaggat ggagcaaaag aagtgaaatt ccgtggtgtg
      361 gcgggtcgat agcgaccgtg acgaccgtat gctggtgcga acgcgatgag gtgaaagcat
      421 ggctcgcagt tgggaagatg cgtgctaggc tggcaaccgc gtggccgtcg gagcatcata
      481 cgatgaacac ggatttcgga ataggatcgg accatcgaga tgagtgaatg acgatggccg
      541 tgctgtgacg atagggagat acgagagcat gacggacaag aagttgcgca tcttgatcgc
      601 gaagccaggg ctcgatggac acgatcgagg ggcgaaagtc gttgcacgag cgcttcgcga
      661 tgcgggattc gaagtggtgt acaccggatt gcaccagaca cccgcgatga ttgcctcggc
      721 ggcggtgcaa gaagacgtcg atgcggtggg cctttccatc atgtcgggag cgcataacac
      781 actgtttccc gcggtcatgg aagccattgc cgaaaaagga gtcgacgata tcgtcgtatt
      841 tggaggcggc atcatccccg atgaggacat cgccacgctt cgaagcaaag gtgtcaaagg
      901 cctgtttacg ccaggaacgt cgctccaaga catcgtcaat tgggtccgtg aaaacgtaca
      961 gccccgcgcg ggatgaggcc gttgctcatc tccctcgctt gaaccatcgc acgcgtcgtt
     1021 gcgcttgtgt gagaggccaa cgataagaaa tatcctcctc actcacggcg tcgatgggtg
     1081 gcgatatctc ctgacgagcg agcaataccg ctacttttcc cttcgaatcc acaagatgca
     1141 agcccatggc aagccatgcg gatggttcga gcgtcgcgcg ggagatggcc acgtcccagc
     1201 cgtgatgagg gaggttttcg ccgtgcgtgc gttggatgga tacggttgtc ggcaaggaac
     1261 cggcgacggt tcgtaaaaag gcaacccgtt ttgcgagagg ctcgaccagg gttacggtca
     1321 agtcgggacg aagcaaagca aggggtaaac cgggtgcgcc cgcgccgctt cccacatcga
     1381 ccacccgtcg attggcctcg atgcttcgtg acagaacgat ggcgtcggcg acgcacaaat
     1441 cgagcaattc gtcgatggtt ctcgcggcgg tcaagtcaat tttttgattc caatgtagga
     1501 tggtttcgat ccactgaacg agttggaggc ggatggtagg gtcgaggggt gtctcgagag
     1561 catgggccaa ctgttcgaga cgcgacggga ttgtgtcgga tcgagggggt agggggcgac
     1621 gattcatggt agcgcggggg ttgatttacg gtgcgattgt tcagcgatga gcctgccaca
     1681 tagtcccacc ttccatgcgt ggaagcgagg gtcactgttg acagaatggg gtcagttccg
     1741 tacttttgcg gggcttggtg gggagaagtg tcaagaagtg gatcatacgc cccatttagc
     1801 ccataagtga tggcaatcat gttccgaggg cagtttacgc attcgatcga cgccaaaggg
     1861 cgcacgagtc tgccttctcg ctttcgggac acgatgggtt ctggagaacc tcgattggtt
     1921 ctcacgcccg cgcttttcga cccttgcatc cacgtatttc cttttgaggc gtgggaagcc
     1981 ttcgaagcgc gggtcgccga gatgcctcaa ttcgatccca atgtcgttcg gctgcgacgt
     2041 ctgtatgtat ctgccgcggt cgagtgcgac ttggacaaac aagggcgcgt actcgttcct
     2101 gttcatcttc gggaacatgc agcgctccag cgggaggtcg tgtgggccgg cgtaggaaac
     2161 aaggccgaac tttgggcaag cgaacgatgg gtcgacgcca caaagatgac gccggagaac
     2221 tgggacgacc tgaagaaggc aatgtccgaa tggaggttgt gatgtgggct gacgtcgagg
     2281 attctcctat ggaacatgag gatttaggat acgcccacac caccgtgctg cgggatgaaa
     2341 cggtatccct tctcggattg cgcgccgggg gagtctatgt ggatgccaca ctggggggtg
     2401 gcggacatgc agaagcgatc ctcgaggcgt cgccaggtgt catggtggtg ggagtggatc
     2461 gagatccggc ggccatggcg gcggcatgca agcgattgag ctgcttcgga accagattcc
     2521 aagggatcgc gggcgctttc gccgatctac ccacgctact gtccaacgcc gggatccgca
     2581 aagtggatgg aatcgtagcg gacttgggtc tgtcgagcgc ccaactcgcc gatgccaacc
     2641 gcggcatgag ctttcgtctc gatggccctc tggacatgcg catggaccct tcgttggaag
     2701 aaaccgcgtt ggacttgatt cgtcgtctga gccaagaaaa tctcgccgac ctgatctatc
     2761 gcttcggtga ggagcgtcga tcccgacgcg tagcccggtg tgtcaagctt gctttgcaag
     2821 acgagcgatt gaataccacc cgggacttgc gtcgtgcggt cgttcgcgcg gtaggaccgg
     2881 ggcgtgtggg gggtgtggac cccgcgacac gaacctttca ggcgctgcga atcgcggtca
     2941 atggagagtt ggaccagctc gcggcgttgt tggagcgagc gccgacggtt ctgacggata
     3001 cgggggtgct tgcggtgatt tcgttccatt ctctcgagga ccggatggtc aaacgagcgt
     3061 ttcagaaccc ttcggtttgg aaggcgctta cgaaaggacc gatcacggct tccgaggagg
     3121 aagaaaacca taatccgcgc gctcgaagcg ccaaattgcg tgcggcccga cgcgtctctg
     3181 aacatgagga aaacccgtga accgttcgcg cccctttctc atcctctgga cgttggctgt
     3241 gttcgctagc gttgcggcct tcgtattgca cctcggctta cgaagccgaa cgttcaaact
     3301 cggttatgaa atcgggcgtg tccggaatga gcaggcacaa ctcaaagaaa ccgtgcatgc
     3361 cctgcaactc gaagccgaaa gctatcggaa ccacgaacgt gtttcgatga tcgccaaaac
     3421 cttgctcggt atgcagccac ccacccggat caacaccatg cgggccgtgg tggttcccga
     3481 cgaatccaaa acgacaacca ccgtacatgc ccagcggtaa ccatgaaaaa cctcagccct
     3541 cgtcgcgcca gtggaattcg tctccgaatg gccgtgctct gtggattgat gggcatcggt
     3601 cttggcgtgg tggtcagcgc agcgtatggc atccaggtcg agcgcgggca ggaatggcga
     3661 gaactagctg aaaaacagcg tcaacgacgc cttcatatct cgccgcggcg cggtaccatc
     3721 tttgaccgca atggggcaac cctggccgac agtgtcgagg ttccgagcgt ttcggtcgat
     3781 gccttcgaga tgtttcgcaa cgtcagcgac caacaattcc cccaattcct cgacaatgct
     3841 tcccagcgtt tggcgaaagc tctatcgctc gatcctgacc aggtgcggga caaactcgcg
     3901 cgacgacgtc gttttgcttc tttgaagcga cgggtgacgg tggccgaggt cgaagcgatt
     3961 cgaaagctcg agcgcgggga agatgggggc cctcgtctcg tgggtctgat ggtggaaggg
     4021 gaaggacacc gatacttccc gaatcgagag cttgcaggtc cgatgctcgg cttcgtggct
     4081 cccgatggag aaggcaaaga tggcatcgaa ttgacgctca acgagcaact tcgcggtcat
     4141 gtcgaggaag tgcgcggatt gcgtgaccgc gctggccgat tgatcttctc ggaaggcatc
     4201 aaaaacgaaa aaacccttgc gggatacaat gtgcatctga cgatcgacaa ggggatccaa
     4261 ttcgcggcag agcgtgaact cgaggccgcg gcgcatacgt tcgaagccaa atccggctcc
     4321 gtggtggtcg tcaatcccca aaccggcgaa atcctcgcca tggcgagtta cccctcgtgc
     4381 aatcccaacg atatcgccca aagctccctg gagtctcgtc gaaatcgagc gatcactgac
     4441 cagttcgagc ccggttcgac gatgaaagtg ttcacgatgg cggcggcgtt ggcgcataag
     4501 gtcatcacac ctacgcagcc gatgtattgc gaagagggcg cgatggcgat cgacaacgtg
     4561 ctcgttcgcg acacgcatcc ttccgcatgg ttgacaccca cccaagtgct ggcgttgtcg
     4621 tcgaatatcg gcgcatccaa gcttggtctc gggcttggag accgaaatct atacgaagca
     4681 cttcagcgat ttggtttcgg aaaacagaca ggtgtgcctc tgccgggaga aactcccggt
     4741 gtgttgcgcc cgcgggatcg cccttgggtt caggtagaaa ccgcggctgc ttccttcgga
     4801 caaggcatca gcgtgaccac cttgcaaatg gcgatggcca cggcggccat tgcgaaccga
     4861 ggccgattgc tgcgccctat tctggtcaaa cggatcacgg atggctctgg ggccgtcgtg
     4921 gaagaaacac ccacggaagt gcttcgaaat gccgtatccg ccaatgtagc gagggtggta
     4981 tccgagatga tggtggcagt gacggaaggg gagggaacag gcaccgaagc cgccgttcca
     5041 ggcttccgaa ccgccggaaa aaccgctacg gcacaaaaaa ccgaccccgc caccggtaaa
     5101 tattccgccg acttatatac cgcttcgttc gttggattcg tccccgccga taacccccgc
     5161 atcgtcgtcg ccgtcgtgtt ggatgaaccc atgatctccc atgccggtgg cgccgtggcc
     5221 gcccccgtgt tccgacgagt ctgtcaagct tctcttcgcg tattgggcgt tgtcgaggag
     5281 caaggtcccc cggtcgcaca aatggaagat gtcgtggacc ttgcggaaga aaactacgcc
     5341 atcatgctgc ccaaaccgcc gattcccgcc ctgaatcccg tggtctcgac ctcgggctcc
     5401 gaccaaggcg ccaaaaccgt cgtggatgtc accggcttgc ccgccacctc cgccctgcaa
     5461 accctgatga aacaaggctt cattccccaa atggtcggct ctggaaaggt ggtgcgtcag
     5521 catcccgccg ccggtgccgt cgtccctccc ggcactgtcg tccagttgta cctagagcca
     5581 ccgacatgac ttcacccctt cgcgccgacg gtgcccttct ttcccaaatc gcccagcgac
     5641 ttggaggtac ccttcatgga aaagaagatc cccggatcac cggcttgcac cacgattcgc
     5701 gaaaaataga acctggtgac ctcttcgtcg ccaggcaagg tgagcgttcc gatggctccc
     5761 agttcatcac ccaagccgtc gagcaaggcg ccgtcgctgt gatgaccgca accatgcccc
     5821 ctacggaacc agcggttccc gtgctgctcg tcgacaatgt gcgcaccgtg ctcggccaag
     5881 cggccaacgt gatctacgcg gacccaacct cccgcctcga tgtggtgggt atcacgggaa
     5941 ccaacgggaa aaccacgaca agctacttgg tgcgggcggc cattgatggg gcgggaggcc
     6001 gtagcggggt gctcggtacc ctcggggccg cctttgaaaa cctgcatttt cccgcttccc
     6061 acaccactcc cgaagccgat gaagtggcaa gggtggcttt cgccatgaaa aattacggag
     6121 cctcccacct ggtgatggag gtctcttccc acgcgctcga tcagcgacgg gccgatggtg
     6181 tgcattttcg agtcgccgcc ttcaccaacc tgacccagga ccacctcgac tatcacggta
     6241 cgttgcaggc gtatgcagca gccaaagacc ggttgttctt tgacctcgac ccccgcgccg
     6301 ccgtcgtgat gatcgacgat gaccacggcg ccgagctggc tcaacgtgtt gcgcggcgca
     6361 atccgaacac tatccgcgta tccaccgatg caaaggggca tgccgatgtg cgacccgaag
     6421 gcgccgtgca tttccatgcg cgaggacttc ggtgccgcgt gcgaacgccc caagggcttt
     6481 tcccgctgga cgcaccttac ccaggcaagc ataacctgtc gaatttatta ttaacttttg
     6541 gaattctgtc ggccttggac ctgtcgatcg aacgagccgc acaagcgctg cgcaccagcc
     6601 ccccggtacc agggcgtctc gagcgatgcg atgacgacct ggacgatatc acggtgttgg
     6661 tggattacgc ccacacgccc gacgcgctgg aacgcgcgtt gcgcaccatt cgtccgttga
     6721 cccaaggaag ggtgctttgc gcattcgggt gcgggggtga tcgggatcgg tccaagcggc
     6781 ccttgatggg cgacgtcgtg ggtaggctcg cggatgtagc ggtcgtgacc aatgacaatc
     6841 cgcgcacgga aaacccgcaa tccattgtag acgcgatcct gcaggggatg actcatgcca
     6901 acgccgaggt gatcgtcgaa ctcgatcggg ccctggcgat cgacaaagtc gtgcggcttg
     6961 ctcatcccca cgatgtcgtg ttgatcgcgg gaaaaggcca cgagcaatac cagatcatcg
     7021 gcgagcagat tcatcacttc gatgatcggc agcaagcgcg cgaggcgctc gaacgaaggc
     7081 gtttgggggg aaaggacgac ccatggcaac gatgattcct cccaatcggg cagcgttcga
     7141 gctgcagcag ctcgtcgcgt tcgtgcaggg cgagtgggat ggttcgggct gcacgacagt
     7201 cgagggggtg agcaccgata cgcgaaccct ggtgccgggc aatgtcttcg tggcgctggt
     7261 gggtgagcgt tttgacggtc atgagcatgt ggaagcggcg atgcgggcgg gggcgcgcgc
     7321 ggttgtggtg tccaggccgg tcgaggtccc tggcggttat gcggtcattc gggtgaaaga
     7381 cacgctcgag gccttgggaa agctgggatg tgctcaccgg caacgttggg ctcgacaggc
     7441 gagggccaat ggtgaggaca aaaaaatcgt ggccgtcacg ggttccgccg gcaagaccac
     7501 cacatgccgc gcaaccactg ccgtgctcga tgccgtgggc aacgcatcgg tccatgcctc
     7561 cgccggcaac atgaataacg cggtgggcat cccgatggtg cttcttggcc ttgactcatc
     7621 ccatggacag gctgtcgtgg aagtaggcac caaccatcct ggcgagatcg cttatggggc
     7681 gagcctggtg cagccggacg tggccgtcgt caccctggtt gcttgtgctc ataccttggg
     7741 tttgggaacc atcgacgatg ttgcgaacga gaaggcggca ttgttggctg ccctggactc
     7801 tacgggcgtg gctgtggtca atgcggacga ccctttggtg atggctcggg tgggacgaac
     7861 gcgagcgcgg cgagtgttga cgtttggcaa gtccgagcga gctgcggtgc ggatcgtcgg
     7921 ggtaaagagc cggggatggc aagggcagac cgtggtgtat gccgtgggcg atgggtcccc
     7981 gaatattgaa tttaacgttg atattccact acttggagat gctggctcct atgcgacggc
     8041 agcggctttg gctgcggcat ggggggtatg gggtgacgcc atggattggc agcgggccgt
     8101 tcatgggttg caagcgttgc gttccgagtc ggggcgattg cgtgtcgtgc cgttgcgttg
     8161 cggcgcgatc gtgatcgacg atgcctataa cgccaatccc gcatcgatac gggattcggt
     8221 ggatgtggcg gcgaggatgg cgttggagcg ggggggggga ttgaccctcg tccttgggga
     8281 gatgcgggag ttgggtgagc atagcgaaag ggagcatcgt cgggtgggtg agcaggtggg
     8341 caaggtgcgg ccttcggcgt tcgtggcggt ggggggggat gcctggttca tggcggagca
     8401 agcccgggcg ctaggagcca acgcggtgtt tgtcgacgac gcggagcgtg cggtggcggc
     8461 ggttcttgaa cggataggag gcaaggatgt gattctggtg aaagggtcgc gaggggttgc
     8521 gctcgagcat gtcgtgggag ccttggaggc atggggggag gggcaaagcg catgatctac
     8581 gagcttttgt attccttgcg cggagaagcc tcgtttttga cctggctcaa cgtgttgcga
     8641 tacatcccct ttcgcgtgat tgccgccacc atcaccgcga tggttctcgc tttcttattg
     8701 gctccttggt tcattcggga attgcagcga aagcaaatag gccaagtggt tcggcaagat
     8761 ggtcccgaaa gtcatctatc caaagcgggc acccccacca tggggggcgc tctgattttg
     8821 ctgtgtttgt tggtgcccac gatgttgtgg tgcgatgtga gaaatccgtt cgtcttggcg
     8881 acgacggcgg tgacggtcgg ctacgggatg attgggtatc tcgatgacta cttgaagctg
     8941 aagcgaaaaa gctcgggggg gcttgcggga aaatacaagc tattggggca gtttttgata
     9001 gcgggggcgg ccattggcta cacgttcgtg tggacggagg ggcttcctgc cgattggctc
     9061 gagattcgaa cccggttgtc ggttccgttt atggctttca acaaataccc catcaccctg
     9121 cccgtcgcgg tgtacgtggt gtttgcggtg ctggtggtgg tggcctggtc caacgcggtc
     9181 aaccttaccg atggcctcga tgggcttgcg atcggtccgg tgctcatcaa cgccagcgcc
     9241 tacttattcc tttcctacgt ggccggtgcg gttttcttcg gcttttccgt cgccaagtac
     9301 ttggacattc cccccttgca aagcgcatcg gaactcgccg tgtattgtgg tgccatggtg
     9361 ggagcgggta cgggtttcct ttggtacaac acgtatcccg cgcaggtatt catgggggat
     9421 gtgggctcgc tggcgctggg cggtgggctt ggaatgctcg cggtattcac caaaaacgag
     9481 ctgctaagca tcgtgttggg cggcgtcatg gtgctggaaa ccgtgagcgt ggtcacccaa
     9541 gtcgtttcat tcaaactcac gggtaaacgt gtctttctca tggctcccat ccaccatcac
     9601 ttcgaaaaga agggatggcc ggagccacgg gtcatcgtcc ggttctggat catttccgtg
     9661 ctgctcgcct tggtgagcct gtcgtctctc aaactgcggt gaaaaaggaa ttgaaaagca
     9721 tggatctacg agacaagcgg gtgatcgtcg tggggctcgg tcgaagtggt atcgcggccg
     9781 cgcggctttg cctgtctcgt ggagcgcggg ttaccgctac cgaccggctc gaacgggcgg
     9841 ccgttgccaa cgatatcctc gaattggaga accagggcgc tcgtttggtg ctgggggggc
     9901 atgaaggatt ggtgtggaaa gatgccgata tcgtggtgat gtctccgggt gtgccaccgt
     9961 ttgccgagct ttttcaagcg gagcgcgcag gcgtgcaggt cattggggaa ctggaactcg
    10021 cttgccgatt ccttcatgcg cccctgggcg tgattggagg aacgaatggc aaaagcaccg
    10081 tcaccagttg gttggggcaa atggtgcaag accgcggcaa ggttttcgtg ggaggcaacc
    10141 tcggaacgcc tttttcctgt catgtcgatg agcgctggga ttgtgtcatc ctcgaggttt
    10201 cgagctttca ggcggagcga gttcccacgt tgcatcctcg cgcggcggcg ttgttgaacg
    10261 tgaccgacga tcacctcgat cggtacgcga gtttggaaca atacgccgag gccaaaggca
    10321 atgtatttcg gaacatgggg cccgaggacg tggcggttgt gccggcgggc gacctcattt
    10381 gtgccaaaca ggcggcgcgg ggacgcgcgc ggatcgtgac gtttggccct ggcggggacg
    10441 ttcgaccgga acagggcgag atcattcact cgcagaaggg ttggcgattt gcgctttccc
    10501 gcatagcgtt gccgggccag cataatgtgt tgaatgcttg cgcggcgatt gccatggcgg
    10561 cggcgctcgg tgccagcgcc gagtcggtcg agcggggcct cgtttcgttc gaaggcttgc
    10621 atcatcgaat gcagtttgtc gccgaaatgc agggcgtgag ctattacaac gactcgaagg
    10681 gcacgaacgt gggagcttcg gtggcggccc tgcggggctt gacggcccgg cgtgtggtgc
    10741 tgatcgccgg aggaagagac aaacaaggaa gttatgaacc gttggtacag gtgttgcggg
    10801 agcgcggacg tgcggtggtg ctcatcggcg aagccgccga ccggattgcc gaggcggtgg
    10861 cggatgcggt gcccacgttt cgggcttcgg acatggacga cgccgttcgg attgccaagc
    10921 aagctgcctt gtctggtgat gcggtgctgc tgagtccggc ttgttcgagc tacgatatgt
    10981 tcaaagacta cgcgcaccgt ggggaagtat tccgctccgc cgttcataac ctaatgaaat
    11041 cacacggaaa ggaggcccca tgagctggtt gcgaaagcga atgggtgctg ccgaccagac
    11101 ccacgtgggc gcggtcgacc ccgtgctcgc cgccaccatc atcgccctcg tcggcttcgg
    11161 tatcgtgatg gtttacagcg ccagcgccat cgaagccacc gtccgatatc acgaccccga
    11221 acactttctc aaacgccaat tcatgtttgg aatcgcaggg ttaggggtca tgggggccgt
    11281 gagctacatc gactaccatc ggcttcgacg gctgacctat cccgtgctgg gtagcgtcac
    11341 ggtgctcatg attctgacgg tcattgggat gggggtgcgt cgcggcggcg cggcgcgttg
    11401 gctgaccgtt gggcccattc tcattcaacc cgccgagatg gccaaactgg ccctcgtcgt
    11461 gtggttggcc tattccctcg cgaaaaaagc agacaaggtg ggctctttca cggtgggatt
    11521 cattccccat gtgctcatgg ctggaggcct catcctgctg tgcttgaagc aaccggattt
    11581 cggctccgcc gtggtcatcg cttttctcac cttcgcattg ctattcgttg ccggggcgcg
    11641 aatggcctat cttttcgggg gactcattgc ggtgatagcc atcggtgttc agctcgtcat
    11701 gagccagggc tatcgtatgg ctcggcttac cgcctacctc gatatcgcca aacatcggca
    11761 gggttccgcc taccagcctt tccaatcggt gatgagcttc ggttccggcg agatcacggg
    11821 gttgggtttg ggaaaagggc ttcaggtgct ttacctgccg gaggcgcata acgacttcat
    11881 tgcggccatc attggagagg aactcggctt catcggcgtg ctgggtctcg ccagtgtgtt
    11941 tttgctcatc gtggcaagag gcgtgagggc tgcgttccgg gcttccgacg actttggttc
    12001 ctacttggcg ttcgggatct catcgatgtt ggggattcag gctctggtca acatggccgt
    12061 ggcgatggcg attcttccga ccaaagggtt gaccttgccg ttcatcagtt acggtgggtc
    12121 ttcgcttttg gtgaatgcag cggcggcggg ggtgctgctc aatatctcca gaccgcgtac
    12181 ccagaggcaa gaagtggagc ctgcgcgcac tgtcccggaa gatgcggaag cgagcgaaat
    12241 tcttatggtt tctgctcagg agggcgcggg gtgagcgtct cgttgctctt tgcgggaggc
    12301 ggaaccggcg gccatatttt tccgctcttg gccgtggcgc aagcgatacg ggcgcggcga
    12361 agcgataccc agatcgtgtt cgtgggcacg gcgcggggaa tggaatctcg gattttgggg
    12421 cagcggggcg agcgtctcga gttgctcgac attcttccca tcaaaggtgg gggatggcgt
    12481 ggggcggctc ggggtatcgt gcgagcggcc gagtcacttc cggcggctca caggcttgta
    12541 tctcaggtcc aaccgaaagc cgttctatcg ttgggagggt acgccgcggg gcctgtctcc
    12601 cttgccgcgc gaacgaaagg aattcccctg gctttgctcg agcccaacag cgtattgggt
    12661 ctcgctaacc tgcttctggc acctttcgct caccgcgcct atctggcgtt tccacaagca
    12721 aaacgctggt ttagaaagtc ggtggtgcga aacaccggcg taccgttgcg ctcgggtttc
    12781 gagccgcaag cctacgagcc cacgcccgat cagtttcata tcctcgtgct tgggggaagc
    12841 caaggggccg tgacgttgaa tcgaaatgtg cctcaggccg tggggttggc gcgggagcga
    12901 acgaaaagcg ttttgcacgt ggtgcatcaa agcggccatg atcggacggc ggaagtcgag
    12961 caaacctatg agcgggcggg actgcgcgat gcggtgcggg tcgtgccgtt cattgacgac
    13021 gtgcctaggg ctttggctcg cgcggacctg gtgatccaac gcgcgggagc cagtgctttg
    13081 gcggaaacct gtgcgatcgg caggccctcg ttgttggtgc cttatccttt tgccgcgggg
    13141 g
//
LOCUS       DAOSOR010000092        13090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4771_length_13090_cov_4.284235, whole genome shotgun sequence.
ACCESSION   DAOSOR010000092 DAOSOR010000000
VERSION     DAOSOR010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 13090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13090
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4771_length_13090_cov_4.284235"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /locus_tag="PK710_07080"
     CDS             complement(<1..1303)
                     /locus_tag="PK710_07080"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOT09514.1"
                     /translation="MSKLRPAPFTLIVMVAAVSCFHGEKAPTVTPNHTLSLAQLENAH
                     ISDQPFGVVFGAPQGETTDAAEVSLVFNRPMRPLGLAGEEAPSPAKISPEIPGAWRWI
                     GTHGLYFAPEGHLPKATDFIVTVPQGTRCLDGSTLPSAYELRFSTQRPQLVSTTPWNG
                     STGLTPAAHFALQFNQPIADNELRRAIKITALAKGTPIPFEVRRPDTDNPKLAELVPT
                     RPLPLAAKIQLTVDASIKGIEGPLTANAPAFFEYETYGPLQVAETYCPKDTPKGKCAP
                     RNSVSIGFTNPVAYAEAKKAISVTPALPLKWWEGAGDDYETERIQIDGAFRPAGTYTI
                     RINGNLRDKYGQRLGRDQVIQLEFDDLWPQAEIGVRGTYFEPTSTRDIAVASVNVPSM
                     DLLTVKVDDDGLRLLSSATRPSFEQLSKLAGSKLTRVHPAAA"
     assembly_gap    1756..1855
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1856..3171)
                     /locus_tag="PK710_07085"
     CDS             complement(<1856..3171)
                     /locus_tag="PK710_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HOT09515.1"
                     /translation="MQSATSMPTDRGRKIVGALLYLLLLLTGVGLLLFLFALAPLFEQ
                     SRCGVGNYFYYMFIGACLAFPAVIAYLFVPVVIDRFDPEPWWALLLAFIWGGFAATGL
                     SGAINTFLGIAGGLVGGDVGSAFIGSVISAPIFEEFWKGLFVFGVFIFLRTEFDGIVD
                     GVIYATFAALGFACVENVIYYGNSGFAAFNQSACNDDKAWEAITGTFFVRGVLSPWGH
                     PLYTSMTGLGLGIARETTRLWLKILAPLVGFILAVTLHAVWNGSALLSAILNLPVIAL
                     TILTYIFFVFLFTILMIGLVVREGIIIRKFLTDEVAIGTISQQELQWVCSPIGRTKAM
                     FSRGMKGRAFVHSCATLALKKWHVARATKGKKRTISVDFIVPLRQEIARLRQQSGAAS
                     PQPAAAGPIGAYPPQQGYPPQQGYPPQQGYPPQQGYPPQQGYPPQQG"
     gene            3427..4551
                     /locus_tag="PK710_07090"
     CDS             3427..4551
                     /locus_tag="PK710_07090"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOT09516.1"
                     /translation="MDLLDQNRRYDFDRVFRLIVAVASIVGGYWLLAYLSEILVPFGI
                     AFVLAYLLNPIATKIQKKTGNRILAVALTLLIFLGALVLVLGLLVPRVISELSGISQF
                     LAEYAHKGPEVWQQMRERLPPQAITVLQDLLRSEKMQDLAALAAQKAAPGLWGVVTGA
                     MSTIVALMTVIIVLMYLAFLLIDFQRVQSSWKEYLPPNHRDAIVEFLEDFNTALSKYF
                     RGQTIVALAVGILFAIGFSIVGIRLAIVLGLFIGLLNMVPYLQNVALVPAFLLALLRY
                     LERGEGFWWYIIGVALVFIVVQIIQDAILVPKIMGDVTGLRPAVIMLSIFIWGKLLGF
                     MGLLLAIPLTVLAVTYYERLIERQRQQARRLESERANGST"
     gene            4666..5466
                     /locus_tag="PK710_07095"
     CDS             4666..5466
                     /locus_tag="PK710_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09517.1"
                     /translation="MSGERLVAVSTTAPPSSVMGSRSANGPDTGEPRECSVVESYLHD
                     PSLRYTSVAFAGPSVLQAVLMMGGFVAGLALFVTPIVRQLSLSEFSFWSLASILGIPI
                     CLELLVPLRDMWRELHHRLRFRVLFRPVELGLERAVPQPATTLRYEIHLSPRRKVKLD
                     AVQMRLVFWESWQAQSRLRFFRLPRRSIEKQGHDLVRQQMGPIQLDKKQHAVIRGSIR
                     IPVARPSEHHRGKRKHISYVNLTITLESQRPGQTIRGNCPHLVTFPWM"
     gene            5545..6744
                     /locus_tag="PK710_07100"
     CDS             5545..6744
                     /locus_tag="PK710_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09518.1"
                     /translation="MVQLLHIHPDSFLVDSFRLGKKVYLSGFRPKHAISLWRGGTPVG
                     LGVDAFFRSRGLRINHTTIATDSYVGISQQAQVTVKNLEHLVQVVCPEDGLLIIDDVY
                     ESGNTIRRVVELLRQKARENAPKDIVVAAVHSKPGRSSYHELPVIALEEIADDVWIDY
                     PHELADLVDPSDPEDRRIREKDEEIWRILRSGPSSRSEVERTGAYTYFSPREMLLDSV
                     RLGVNIAHDRSFRPDFIIALWPGGVHAGLPIHEVYKYFQAKAGGVGKTPDHISVNTYP
                     TRLSYRTQILGLHYLEDHINKDDNILIVDTTFRAGRLVNAVVASLKEALRRNLDLERV
                     RVASIYFNPDDRSTWTVRPDIRRPDYFLRTVRNEVVYPHSIHNFPNPRKDLAQLNPSL
                     WNVLYED"
     gene            complement(6748..7317)
                     /locus_tag="PK710_07105"
     CDS             complement(6748..7317)
                     /locus_tag="PK710_07105"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOT09519.1"
                     /translation="MTSPIFAHHGKYYRETASAEPWKLEPARHNGGVPLVMHVKRIPT
                     GRRQQLARQEFEMADNNSDNANQDRRREPRHLACFPAYIEDAGVTRSAIIRDLSITGA
                     RLLTRARPEVGDRVRLSLYITANSEQPGTATGNVVRVEPWGDGSTLWSFSVAVEFDEP
                     ATEHEEAIKALAERQAKLGLFKTADPERR"
     gene            7376..7822
                     /locus_tag="PK710_07110"
     CDS             7376..7822
                     /locus_tag="PK710_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09520.1"
                     /translation="MRIPRKISCVGQVVTPNSVTNPFTGARAAAFAFLIRSPSGNVCT
                     KVLGSDIVVHCPMGNILVGLRNSHVRTMRATAEHPIPRPWQEPLAVEPGSSYRQTAIE
                     HGARVLLAAHVRMVSRSRHTEVVYWWTTCPGFGDTKLTLLDPSVER"
     gene            complement(7823..8503)
                     /locus_tag="PK710_07115"
     CDS             complement(7823..8503)
                     /locus_tag="PK710_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09521.1"
                     /translation="MRTEQPPRWLGRGIPPSQPSPSYLRIPLKPPGGKKPSNPVIVAI
                     LSLTIVAAMAVGIWFARRRSERVVGSPQLVMSGLVQGAASIDPLGLVIASSETLTIRT
                     DQVIIEKVWVDSCQRPGLGRTPAARCDRQPFFERSLVKAVLQGVNCVPEQRREESISF
                     ALEINHKTRTTRLFAGKSGSLTRTKVKPVLECIQQSLASADWEQIPHEHSRYVIGVLA
                     KYPVQKRD"
     gene            8690..10768
                     /locus_tag="PK710_07120"
     CDS             8690..10768
                     /locus_tag="PK710_07120"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HOT09522.1"
                     /translation="MQRSTLKTPMCRFPLVCLGLAALFLACGESSEESSQGKAGASGN
                     TDGGVGGSSGKGGSGGTGGGSATGGTSGTGASSGSGGGANVGGSGGAETGGSGGVAEC
                     SKDEDCAGKTQTPFCSAQGACVACEPGVQAHTCEGGLVCCDFQCVDTLFDIHNCGECG
                     KSCSLPNASAKCAMGQCRIDSCDSGFADCDGLPLNGCESNEGSCSCTPGDVVECYSGP
                     PGTQGVGPCKAGQRTCNATGTGWGPCEGEKLPSPDSCLNNIDDDCDGIVNNGFPNAPG
                     CVCIPNEEKPCYSGPPSTLNVGTCHGGMAACEPSGIGFGLCVGEVVPKNEEVCFNGLD
                     DDCNGVVDDSPDADGDGWTVCDGDCCDSVGTTCGTPALVNPGAIEVVGDGVDNNCNGQ
                     VDEDPLSACSSGSKFSGTTAMDLLNAMEICKVSTGGSWGIVGTPVISRANGSGAGTVS
                     NLQVAVTTQFGTDSSNLPRFGPNFAMLSSGHARDANDPDATAGLSYTYTTGAPPADFV
                     APHGGSLPSTGAGCPIGSGANDSVMLDVRLKVPTNANSFSFQFRFFSQEYWTYTCTQY
                     NDFFIAMLDSSWLPGPGQTAIPADKNISFDNNKNYISVNSHQFFTVCPPKSGYPCPNG
                     QAGLTGTGYTYGPGGGTVWLTTTAPVVPGETIRLRFATWDTSDMLLDSIVLLDNFRWS
                     AAPSSGPVTE"
     gene            complement(10814..12244)
                     /locus_tag="PK710_07125"
     CDS             complement(10814..12244)
                     /locus_tag="PK710_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IG family 5'-nucleotidase"
                     /protein_id="HOT09523.1"
                     /translation="MNRFLDRTSPARRIFCNRTLNMKSIRAVGFDMDYTLIHYHVTAW
                     EGRAYAYVRQRLEADGWPVADLTFDPSLFSRGLVVDEELGNVVKADRFGFIRRASHGT
                     RMLSFEEQRDVYGDVQVDLGAPRWTFMNTFFSLSEACLYGQLVELLDSGALPGVLGYA
                     DLSRRIRSHLDATHMEGELKREIVTAPDRFVDRDADLVLTLLDLKRAGKLLLLITNSE
                     WDYTKAMMQYALDGQLPEGMTWRELFDIVIVASRKPDFFTSRAPAFEVVDEQGLLRPL
                     NHPMAAGGVYLGGNARQVEMDLGLSGEEFLFVGDHIFADVHASKSHFRWRTALVIREL
                     EDEVLALESFKEKQRELTRKMVDKEKREQEHASLRLLLQRMEAGYGPAVEMTASALKA
                     RLQELRKEINELDETIAPLARQAVELVNSRWGLLMRAGNDKSHLARQVERYADIYMSR
                     VSNLLQVTPFFYLRAPRGSLPHDSGA"
     gene            complement(12271..>13090)
                     /locus_tag="PK710_07130"
     CDS             complement(12271..>13090)
                     /locus_tag="PK710_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09524.1"
                     /translation="RDDSTFTAPVDCGTYLIVADTYGTGDANAGPYTLNVSFSATGAS
                     CGNGPPSYHPSGRPGDACGYPGQEHLPFCNANLGAEVCLVTNGTPKTSFCSHPCATAT
                     DCPEFPGGCCKTISSGESYCVTAPLCGGTPGDAGATDGPPSDTHDPDAVSDVLVSDSS
                     NETDAILDSSVEESASDVVTQDSVTPAPDASSADASSDASSDAQIIDTVSTRPVESDE
                     DSQGCACRMRSHPPSRAGIVLILLAGLMLMRRGNRLWTQNQWRAVGGSHNRSLR"
BASE COUNT         2772 a         3675 c         3685 g         2858 t
ORIGIN      
        1 gagcggctgc cggatgcaca cgcgtaagct tggagccggc tagcttcgaa agctgctcaa
       61 agctcggacg agtcgcggaa gacagtaggc gaagcccatc atcgtcgacc ttcaccgtaa
      121 gcaaatccat gctcgggacg ttgaccgacg ccaccgcgat gtcacgcgtc gaagtgggct
      181 cgaaatatgt ccctcgaaca ccaatctccg cttgaggcca aagatcgtcg aactccaatt
      241 gaatgacttg gtctcggcct aatcgctgcc cgtacttgtc tcgcaagttg ccgttgatgc
      301 gaatggtgta cgttcccgcg ggacgaaagg ccccatcgat ctgaatccgc tcggtttcgt
      361 aatcgtctcc cgcgccctcc caccacttca aagggagcgc tggggtcacc gatatcgctt
      421 ttttggcctc tgcatacgcc actgggttgg taaacccaat cgatacggaa tttcgcgggg
      481 cacacttgcc cttcggtgta tctttgggac agtaagtttc ggcaacttga aggggaccgt
      541 aggtttcata ttcgaaaaag gccggggcat ttgccgtcaa tggtccctcg atacctttta
      601 ttgaagcgtc gacagtgagc tgaatcttcg ccgctaaagg caaaggacgt gtcggaacga
      661 gttcagcaag cttgggattg tccgtgtccg ggcgacgaac ctcgaaagga atgggggtgc
      721 ccttggcaag tgcggtaatc ttgatggcgc gacggagttc gttgtcggca atgggctggt
      781 tgaattgaag cgcgaaatgc gctgcgggtg tcaaccccgt cgaaccattc cacggggttg
      841 tgctcaccaa ttgtggacgt tgggtgctga accgtagctc ataagcggaa ggcaacgtgg
      901 aaccatcgag gcaacgcgtt ccttgcggca cggtgacgat gaaatcggtg gctttcggca
      961 ggtgtccctc gggggcaaaa tacaagccat gggtgccgat ccaacgccaa gctcccggga
     1021 tttcaggcga aatcttggcg ggggaaggag cttcctcacc tgccaacccg agtgggcgca
     1081 tcggacgatt gaagaccaag gagacttcgg cggcatctgt tgtttcccct tggggagcac
     1141 caaaaacaac accaaaaggc tggtcggaaa tatgcgcgtt ttccagttga gccagggata
     1201 aggtgtggtt cggtgtgacc gtcggtgcct tttcgccatg aaaacagctc accgcagcca
     1261 ccatgacgat taaggtaaaa ggcgccggac gaagcttgct cacgaatggt cgaatcatac
     1321 cgcgaaaaga atgtgtactc atgggtgtcc ccgggttgag caatgatcgt gcctggaaaa
     1381 atcaaccgca aattcgcata agcactgcgt cttgccatac acaggctcgt ggacctgtag
     1441 acacgcttcg aagcctatcg gtggatgaga cgccaacgaa aacacacgcg atccaccgat
     1501 ggtcgataac gtagcgaaca tgggttggct cgtcgaccag gaatcaatcc aacggttcac
     1561 tccttgccga actcgtgcca tcggcaacga ctcgttacgt ttacctgttc gaagggatat
     1621 gcgttggctt gaaaggcttc gcgcccaagg gattcaatta cctaacgttt ccgcctggac
     1681 cccatcctcc ctgccctcca ggcggatagc cttgctgcgg cggataacct tgctgcggcg
     1741 gatagccttg ctgcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccttg
     1861 ctgcggcgga taaccttgct gcggcggata gccttgctgc ggcggataac cttgctgcgg
     1921 cggatagcct tgctgtggcg gatagccttg ctgtggcgga taagcaccga tggggccagc
     1981 cgcggcaggt tgaggggatg cggcgccgga ctgttgtctg agccgtgcga tttcttggcg
     2041 taacgggacg atgaagtcca cgctgatggt tcgttttttc cctttggtgg ctcgtgcgac
     2101 atgccacttt ttgagggcga gggtcgcgca ggaatggacg aaggcacggc ctttcatccc
     2161 gcggctgaac atcgctttcg ttcttccaat gggagagcag acccattgga gttcttgctg
     2221 cgatatggtt ccgatggcga cctcatcggt caggaatttt cgtatgatga tgccttctcg
     2281 aacgacgagg ccgatcatca aaatcgtgaa taaaaacacg aagaaaatat atgtcaggat
     2341 agtcaaggca atgacgggta ggttgaggat ggccgacaac aatgccgagc cattccacac
     2401 ggcgtgcaag gtcacagcga ggatgaatcc caccaaggga gccaggattt tcaaccacaa
     2461 tcgcgttgtt tcacgcgcta tccccagacc gagcccggtc atcgacgtat agagcgggtg
     2521 gccccacggg ctcaagacgc cgcgtacgaa gaaggtaccc gtgatggcct cccaggcttt
     2581 gtcatcattg caagccgatt ggttgaaggc cgcgaatccc gagtttccgt agtagatgac
     2641 gttttccacg cacgcgaaac caagggccgc gaacgtggcg tagatgacac cgtcgacgat
     2701 cccatcgaat tcggttcgaa ggaaaatgaa tacgccgaaa acgaaaagcc ctttccagaa
     2761 ttcctcaaag atgggagcgc tgatgacgct gccgataaaa gcggagccga catcgccgcc
     2821 aacgagtccc cctgcaattc ccaagaaggt attgatggcc ccggacaaac cggtggcggc
     2881 gaagcctccc cagatgaaag cgagcaacaa cgcccaccat ggctccggat cgaatcggtc
     2941 gatgaccacg ggaacgaaaa ggtaggcaat gacggctggg aacgcgaggc atgctcctat
     3001 gaacatgtag taaaagtaat taccgacgcc gcagcgggac tgttcgaaca acggagcgag
     3061 cgcgaaaagg aatagaagaa ggccgactcc ggtgagcaac aggagcaagt acagaagagc
     3121 gccaacaatc ttgcgccccc ggtccgtggg catgcttgtc gcagattgca taggtagacg
     3181 tatcacacct gaatccgtcc gtccggtcaa cgtagaagtc tgtccaagga cgaaacggtt
     3241 tggcgtgcgc gaggtttcca gcccagaccg cgaacgatag agcgggtcgc gatgagaagc
     3301 cgatgaatga ggagtggctg ccgttaggtc gactccaccc ccgctcttcg ccgctcctac
     3361 ccgaaatttc tcctttgctt tctgctcccg ctacgatggg agcgcccccg accgacagga
     3421 gaaaccatgg acctgcttga tcaaaaccgt cgctatgact tcgaccgggt ctttcgcctc
     3481 atcgttgccg tggcgtcaat cgtgggcgga tattggttgc tcgcttacct atcggaaatc
     3541 ctcgttccgt tcgggatcgc cttcgtgttg gcctatttgc tcaatcccat tgcgacgaaa
     3601 atccaaaaaa agacgggaaa tcgaatactt gcggtggcct tgacccttct gatcttcctc
     3661 ggggcgctcg tgctcgtttt gggccttctg gttccccgtg tcatttccga actgtcgggt
     3721 atctcgcaat tcttggcgga gtacgcgcat aagggaccgg aagtatggca gcaaatgaga
     3781 gagcggctgc cacctcaggc cattaccgtt ttgcaggacc tgctgcgctc ggaaaaaatg
     3841 caggacttgg cagctttggc ggcgcagaag gccgctccag gtctgtgggg agtcgtcact
     3901 ggtgccatga gcaccatcgt cgctttgatg accgtcatca tcgtattgat gtatctcgct
     3961 tttttgctca tcgactttca acgagtgcag tcttcatgga aagagtatct tccccccaat
     4021 catcgcgatg cgatcgtcga attcctcgag gacttcaaca ccgctctttc caagtatttc
     4081 cgcggccaaa cgatcgttgc gctagcggta ggaatcctat ttgccatcgg cttcagcatc
     4141 gttggcattc gactcgccat cgtattgggt ctgttcatcg gactgctgaa catggtcccg
     4201 tacttgcaaa acgtcgcttt ggttccagcg tttcttcttg ccttgttgcg ttacctggaa
     4261 cggggggaag gattctggtg gtatatcatc ggcgtcgctc tcgtgttcat cgtcgttcag
     4321 atcattcaag atgcgatact cgttcccaag atcatgggtg atgtgacggg actgcgtccg
     4381 gctgtgatca tgctgagcat ttttatttgg ggcaagctac tcggtttcat ggggttgttg
     4441 cttgccattc ctttgactgt gcttgcggtg acttattacg aaaggttgat tgaacgccaa
     4501 cgccagcaag cgcgaagatt ggagagcgaa cgagccaatg gttcgacgtg attgcggaac
     4561 gttgaggttc ggcttttccc attatcgttt cgatttcgtt ccgtggcgtg ggcagcgaac
     4621 ggggacgatg caatttgaaa cggtgctgct acagtccatc ctcccatgag cggcgaacga
     4681 ctcgttgcgg tttccacgac ggccccaccg tcttcggtaa tggggagccg ctccgccaat
     4741 gggccggata cgggcgagcc gcgcgagtgt agcgttgtgg aaagctactt gcatgatcct
     4801 tcgcttcgct acacctccgt ggccttcgcg gggccttcag tccttcaggc ggtcttgatg
     4861 atgggcggct tcgtcgctgg cctcgcccta ttcgtgactc cgatcgttcg acaactttct
     4921 ctgtcggaat tttcgttttg gtcgttggcc tcgatcctgg gaatccccat ttgtctcgaa
     4981 ctattggttc ccttgcgaga tatgtggcgg gagttgcatc atcggctccg cttccgcgtg
     5041 ctttttcgac cggtcgagct ggggttggag cgggcggtcc cgcaaccggc gaccactctc
     5101 cgctacgaaa tccacctttc cccccgtcga aaagtcaaac tcgatgccgt ccaaatgaga
     5161 ctcgtctttt gggaaagctg gcaagcacaa tcccgtcttc gattctttcg tttgccgcga
     5221 cgctcgatag aaaagcaagg gcacgacttg gtgcgccagc agatgggacc aattcaactc
     5281 gacaaaaaac agcacgcggt gatccgcggt tcgatccgta ttcccgtggc tcgcccttcg
     5341 gaacaccatc ggggaaaaag gaaacatatt tcttacgtca acctgaccat caccctcgaa
     5401 tcgcagcgcc ccggtcagac gatacgcggt aattgccccc atcttgtcac gtttccgtgg
     5461 atgtgatcgc gcaaccagct agcccccgct tcggcccggt tgatcctctg cccctgttcg
     5521 ttcccctttt gctagcatgc caccatggtg cagctcctgc acatccatcc cgattcgttt
     5581 ctcgtcgatt ctttccgcct cggaaaaaaa gtgtatctaa gcggttttcg tcccaaacac
     5641 gccatctccc tgtggcgtgg agggactccc gtcggcctcg gtgtcgatgc cttctttcgt
     5701 agccgcggtt tgcgcatcaa ccacaccact atcgccactg actcttacgt cggtatctcc
     5761 caacaagccc aggtaaccgt aaaaaatctc gaacacctcg tccaggtcgt ttgccccgag
     5821 gatggcttgc tcatcatcga tgacgtttac gaatccggaa acacaatccg tcgtgtggtt
     5881 gagctgttac gacaaaaagc tcgcgaaaat gccccaaaag acattgtcgt cgcggccgtg
     5941 cattccaaac ccgggcgcag cagttaccac gaactcccag tcattgccct cgaagaaatc
     6001 gccgacgatg tatggatcga ttacccacac gaattggccg atctcgtgga cccttccgat
     6061 ccggaagatc gtcgcattcg cgaaaaggac gaggaaatct ggcgtatttt gcgctcgggc
     6121 ccttcctccc gttccgaggt ggagcggacg ggagcctata cgtatttctc acctcgagag
     6181 atgcttctcg acagcgttcg cctcggcgtg aacatcgcgc atgatcggag ctttcgaccc
     6241 gacttcatca tcgcattgtg gcccggcggt gttcacgcgg gcctgcccat ccacgaagtc
     6301 tacaagtatt ttcaagcgaa agccggtggc gtcggcaaaa cccccgatca tatcagcgtc
     6361 aatacctacc ccacccgttt gagttatcgc acgcagatct tgggcctgca ctatctcgaa
     6421 gatcatatca acaaagatga caacattctc atcgtggata cgacgtttcg cgctggccgt
     6481 ctcgtcaatg ccgtcgtggc aagcttgaaa gaagcactgc gccgcaatct ggatctcgaa
     6541 cgcgtgcgcg tggcgtcgat ttacttcaac cccgatgacc gaagcacctg gaccgtacgc
     6601 cctgatattc gacgtcccga ctatttctta cggaccgttc gtaatgaagt agtgtacccc
     6661 cacagcatcc ataattttcc gaacccgcga aaagacctgg cgcaactcaa cccatcgctc
     6721 tggaacgtgc tgtacgaaga ctgacgttca acgtcgttcc ggatcggccg tcttgaacaa
     6781 acctagcttg gcttgccgtt cggcaagcgc tttgatcgct tcctcgtgct ccgtggccgg
     6841 ctcgtcaaat tcgaccgcaa cgctgaaaga ccacagcgtg cttccatctc cccatggttc
     6901 gacacgaacg acattgcccg tggccgtacc cggttgctcc gagttcgcag taatataaag
     6961 actcaaacgc acgcgatcgc caacctcggg acgcgcccgc gtcaacagcc gcgctcccgt
     7021 aatgctaaga tcgcggatga tcgccgaccg cgtgacgccc gcatcttcaa tataggcggg
     7081 aaagcaagcg agatgtcgag gttcacgccg tcgatcctga ttcgcattgt ccgaattatt
     7141 gtctgccatc tcgaattcct gtcgggcgag ttgctggcga cgccctgtgg gaattctttt
     7201 aacatgcatt accaaaggta cgccaccgtt gtgcctggca ggctcgagtt tccatggctc
     7261 ggcggatgcg gtttcgcggt agtacttccc atgatgagcg aaaattgggg acgtcaagac
     7321 gttaggctgg ccgtggggga gccttgtcgc aaaaaggcgg cgaagaggtg atggcttgcg
     7381 tattcctcgg aaaatatcct gtgtcggaca ggtagtaact ccaaatagcg ttacgaatcc
     7441 cttcacggga gcccgcgcgg ctgcctttgc ctttttgatt cggtcgccct cgggaaacgt
     7501 gtgtaccaag gttttgggta gcgatatcgt cgtgcattgc cccatgggaa atatcctcgt
     7561 ggggttacga aattcgcacg tcagaacgat gcgggcgact gctgaacacc ccatccctcg
     7621 accatggcaa gagccattgg cggtcgaacc agggtcgagt tataggcaaa cggcgatcga
     7681 gcatggagcg cgtgtgctcc ttgccgccca tgtgcgaatg gtgtctcgga gtcggcatac
     7741 ggaggtggtg tattggtgga cgacttgtcc gggcttcgga gatacgaagt tgaccctgct
     7801 cgacccatcg gtcgaacgat gactagtccc gcttttgaac gggatacttc gcgagcacgc
     7861 cgatgacata acgagaatgt tcgtggggga tttgttccca atccgcagag gcgagagatt
     7921 gttgaatgca ttcgaggacg ggcttgactt tggtcctcgt gagcgagccg ctctttcccg
     7981 cgaacagccg tgtggtgcgt gtcttatggt tgatctccaa cgcgaagcta atgctttctt
     8041 ctcgccgctg ttcgggtacg cagttcacgc cttgcaagac ggccttgacc agggagcgct
     8101 cgaaaaaggg ttgacggtca cagcgcgcgg ctggagtgcg tcccagcccc ggacgctggc
     8161 atgaatcgac ccagaccttt tcaatgatga cttgatccgt acggatggtc agcgtttcgg
     8221 aactcgcgat gaccaatccc aaaggatcta tcgacgccgc tccttgcacc aagccactca
     8281 tgaccagttg aggagaacct acgactcgtt cggaacgtcg acgagcaaac catataccga
     8341 cagccatcgc ggcgacgatg gtcaacgaca agatcgccac gatgacagga ttggacggct
     8401 tttttccccc gggcggtttc agaggtatcc tcaagtaaga gggagatggt tgcgaaggag
     8461 gaatccctcg ccccaaccac cgcggaggtt gttcagttcg catgatggtt cttgaattca
     8521 agcatggact gtcgggcatg cgtgaaacaa tcgggtgaat gacgatggtt accggcaaac
     8581 cttccggaaa accttccggt aaaccgcagc agacgagctt tggaaatggc ctgctcgatc
     8641 attcgcttcc tggtatagtt gtttgttgat gtcgcaaacg gaggaatgaa tgcaacgctc
     8701 cacgctgaaa acgccgatgt gccgattccc gttggtttgc cttggtctcg ccgctctttt
     8761 tttggcttgc ggtgaatctt ccgaagaatc ttcgcaaggg aaagccgggg cgtcaggcaa
     8821 taccgacggt ggcgtagggg gatccagtgg aaaaggtgga tctggtggaa cgggaggcgg
     8881 cagcgcaact gggggcacca gcggtactgg ggcgagcagc ggctcgggag gaggagccaa
     8941 cgtaggagga agcggaggag cggaaacggg tggttcggga ggcgttgccg aatgctcgaa
     9001 ggatgaagac tgcgcgggaa aaacgcaaac gcctttctgc tcggcgcaag gagcctgcgt
     9061 ggcctgtgag cccggtgtgc aggctcatac ttgcgaaggg ggtttggtgt gttgcgactt
     9121 ccaatgcgtc gacaccttgt tcgatatcca caattgtgga gagtgcggca agagctgttc
     9181 actgcccaac gcctcggcaa aatgcgccat ggggcaatgc cgcatcgata gttgcgatag
     9241 cggtttcgcc gattgcgatg gtttgccgct caatggttgc gaatccaacg agggaagctg
     9301 ctcatgcacg cccggtgatg tcgtggaatg ctattcggga ccaccgggca cccaaggcgt
     9361 gggaccctgc aaagcagggc agcgtacctg caatgccacc ggcacgggat ggggaccgtg
     9421 tgaaggagaa aagttgcctt ctccggactc atgcctcaat aacatcgacg atgattgcga
     9481 tggcatcgtc aacaatggtt ttcccaacgc gcctggatgc gtatgtatcc cgaacgagga
     9541 aaagccctgc tactcaggtc ctccgagcac gctgaacgtg ggaacttgcc atggtggcat
     9601 ggctgcgtgc gagccttcgg gcattggctt cggtttgtgt gtcggggaag tcgtacccaa
     9661 aaatgaggag gtttgtttca acggcctcga tgatgattgc aatggtgtcg tcgatgactc
     9721 gcccgacgcc gatggcgacg gttggactgt ctgtgatggt gattgttgtg attctgtcgg
     9781 cacgacctgc ggcacacccg cgctggtcaa cccgggcgcg atcgaagtgg ttggcgatgg
     9841 ggtggacaac aattgtaacg gacaggtcga tgaagatcct ttgtctgcct gcagttcggg
     9901 gtcgaagttt tctggaacga ccgccatgga tttgctcaat gccatggaaa tctgcaaagt
     9961 ctcgaccggg ggcagttggg gcatcgtcgg tacccccgtc atctctcgtg ccaatggttc
    10021 gggtgcggga accgtcagca atttgcaagt ggccgtgacg actcagttcg gaacggattc
    10081 atcgaatctt ccgcgcttcg gtccgaactt cgccatgctc tcgagcggcc acgcgcgaga
    10141 tgccaacgac cctgatgcga cggccggcct ctcgtacacc tatacgactg gcgcaccccc
    10201 tgctgacttc gtggcgcccc atggcggcag tctgccttcg accggcgccg gatgcccaat
    10261 tggcagcggc gccaacgatt ccgtcatgct cgatgtgcgg ctcaaggtcc cgaccaatgc
    10321 caactccttt tcgtttcaat tccgcttctt ttcccaggag tattggacct acacatgcac
    10381 gcagtacaat gactttttca tagccatgct ggattcgtca tggctgccag gtcccggaca
    10441 gaccgccatc ccggcggaca aaaatatctc cttcgacaac aacaaaaact atatcagcgt
    10501 caacagccac caattcttca ccgtttgccc tccgaaatcc ggctacccct gtccgaatgg
    10561 acaagcgggt ctgaccggta cgggctacac ctacgggccc ggaggaggta ccgtgtggtt
    10621 gacgaccacc gcgcccgtcg tgcctggtga gacgattcgc cttcgattcg ccacgtggga
    10681 cacgagtgat atgttgctcg attcgatcgt tttactcgat aatttccgct ggagcgcggc
    10741 tccttcgagc ggacctgtca ccgagtgatt ccgtcgattc tttccgcttc cccgacgcac
    10801 cgccgaagct gcctcaggcg ccggaatcgt gaggaaggct cccccgcggc gctcgcagat
    10861 aaaaaaacgg tgtgacttgc agtagattcg atacgcgcga catatagatg tcggcgtagc
    10921 gttcgacttg acgcgcaaga tgactcttgt cgttgcccgc gcgcatcagc aatccccatc
    10981 gcgagttgac gagttctacc gcttggcggg caagaggggc gatggtttcg tctaattcat
    11041 tgatttcttt acgtaattcc tgaagccggg ccttgagggc agacgcggtc atctccacgg
    11101 cgggcccata tcccgcctcc attcgttgca gcagcaggcg cagcgatgcg tgttcctgct
    11161 ctcttttttc cttatccacc atcttgcgcg tcagctcccg ttgtttttct ttgaacgatt
    11221 ccaaagcgag cacttcatct tccaattcgc ggatcacgag tgccgttcgc catcgaaaat
    11281 ggcttttcga tgcatgcaca tccgcgaaga tatggtcccc cacaaacaaa aactcttccc
    11341 cggatagacc taggtccatc tccacctgtc gtgcatttcc tcccagatag accccgcctg
    11401 ccgccatggg gtgattcagc ggccggagca gcccttgttc atcgacaacc tcgaaagccg
    11461 gcgctcgact cgtaaaaaaa tcaggtttgc gagaagccac gatcacgatg tcgaatagct
    11521 cgcgccacgt catcccctcc ggcaactgcc catccaaggc atattgcatc atcgctttgg
    11581 tgtaatccca ctccgaattg gtgatgagca gcaacagctt gcccgctcgc ttcagatcga
    11641 gaagcgtcaa caccagatcc gcatcgcgat ccacgaagcg atcgggggcc gtgacgatct
    11701 cgcgcttcaa ctcaccttcc atatgcgtgg catccagatg cgatcggatc cgacgactca
    11761 gatccgcgta ccccagcacc cccggcagcg cccccgaatc aagaagctcc acgagctgcc
    11821 cgtacaaaca agcttcagag agcgaaaaaa acgtgttcat gaatgtccat cgcggtgcgc
    11881 ccaggtccac ctgaacatcc ccgtacacat cgcgttgctc ctcgaaggaa agcattcggg
    11941 ttccatgact cgcgcgccga atgaacccga accgatcggc tttgaccacg ttgccgagtt
    12001 cttcgtccac caccaatccc cgtgaaaaca aggacggatc gaaggtgagg tcagcgaccg
    12061 gccacccgtc ggcttccaag cgttgccgaa cgtaggcata cgcgcgcccc tcccacgccg
    12121 ttacatgata gtgaatcagc gtgtagtcca tgtcgaaccc cacggctcga atgcttttca
    12181 tgttcaacgt tcgattgcag aagatgcgac gggccggcga ggtgcggtcg agaaatcgat
    12241 tcatgctctt gctgtaaagg aagccagcag ctaccgcaac gatcggttgt ggctcccacc
    12301 aacggctcgc cactggtttt gcgtccacaa cctgtttccg cgtcgcatga gcatgagtcc
    12361 cgccaacaaa atcagtacta tgcccgcgcg cgagggggga tgagaacgca tgcgacaagc
    12421 gcatccttgg gagtcttcat cgctttcgac agggcgagtg gataccgtat cgatgatttg
    12481 cgcatcggag gaggcgtcgg aggaggcatc ggcggaagaa gcatcgggag cgggcgtaac
    12541 gctatcttgc gtgacgacat ccgaggcgct ttcttccacg gacgagtcga ggatggcgtc
    12601 ggtttcgttc gatgaatcgg aaacgagcac atccgaaacg gcatcggggt cgtgggtatc
    12661 tgagggcgga ccatcggtag cgcccgcatc gccaggtgtt ccaccgcaaa gtggtgcggt
    12721 aacacaatag gactcaccgc tggagatggt cttgcagcat cctcccggaa actccgggca
    12781 atccgtggcc gtggcgcagg gatgagaaca aaacgaggtt ttcggagtgc cattggtcac
    12841 caaacagacc tctgcgccaa ggtttgcgtt gcaaaacggc aaatgctctt gtccggggta
    12901 gccgcaagca tcaccgggcc ggccggaagg atggtaactc ggaggcccgt tgccgcaaga
    12961 agcgcccgtg gccgaaaacg atacgttgag cgtataaggg cccgcgttgg cgtctcctgt
    13021 cccgtaggta tcagccacga tgaggtaggt tccacaatcg acgggggccg tgaacgtgct
    13081 gtcgtctcgg
//
LOCUS       DAOSOR010000093        13029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4795_length_13029_cov_3.867350, whole genome shotgun sequence.
ACCESSION   DAOSOR010000093 DAOSOR010000000
VERSION     DAOSOR010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 13029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13029
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4795_length_13029_cov_3.867350"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..1600)
                     /locus_tag="PK710_07135"
     CDS             complement(224..1600)
                     /locus_tag="PK710_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HOT09525.1"
                     /translation="MNLHPAHPRPKTESGSELLDFDQLSWPFSSACKPRHAFRIGIEA
                     ERFGVYSRTLQPLQYDGQDGILGLFHELVSRYGWLAKSESPGGPVVSLKRNGAAITLE
                     PAGQFEFSGSPWHDLHALENERQTYNTELQSISDPMGITWLASGFHPLARHDQLPWVP
                     KLRYGIMRVYLASQGGDALEMMRRTATVQANFDFSSIEDGLRKLRVSLRLSPIVSAMF
                     ANGPFVEGRRYGGKSRRIQAWLQVDPARQGLLPQMWNPSATLDDYIQWALDAPMFLFL
                     RQGKAIENTGQTFRSFLIDGFQGYRPTLEDWTAHLNTLFPEVRLKQTIEVRGADSVPN
                     RYTPALPALWTGILYDDKALDAAERLTETWTYPSMVTLRQSLGNEGLNAMFHGKTVAT
                     WAQRVLSIAKEGLGRRNQRDSAGQAEDIYLKDLEILLEKGRCPADEVLDALEQEPSAQ
                     NIVLATTA"
     gene            complement(1672..2262)
                     /locus_tag="PK710_07140"
     CDS             complement(1672..2262)
                     /locus_tag="PK710_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HOT09526.1"
                     /translation="MARNPYRRPDHRSRQAREQGYPARSIFKLEEIDRRVQLLRPAMR
                     VLDLGAAPGSWSMYASQRVGPIGRILAIDLQPIDYPLGSNVTFLQGDALALQEPALAT
                     FAPYDVVLSDMAPNTTGFSHVDEIRSADLFMRAVDVAASLGCRGSAFVGKLFMGATFE
                     QARQHLRERFNKVRIIRPEGTRRRSVETFLIGIERK"
     gene            complement(2268..2936)
                     /gene="rplC"
                     /locus_tag="PK710_07145"
     CDS             complement(2268..2936)
                     /gene="rplC"
                     /locus_tag="PK710_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOT09527.1"
                     /translation="MNQHPGTIGRKLGMTQFFDDNGIVQRVTVIEAAAVVVGKRTLDK
                     DRYSALIVGLDERKPKHTNKPLGGFFKKAGVTPKRILKEFRLPADVVAQYEIGQKLEL
                     DKVFEVGQFIDVRAKSRGHGFSGVMRRYNFKGQVNTHGTHEYRRHGGSIGTNLTPGRT
                     LKGVKMPGQYGNKQCSVLNLKVAKIIPEQDLLLVHGGVPGSRNGIVVIRGAVKKMGGH
                     PKAK"
     gene            complement(3125..4219)
                     /locus_tag="PK710_07150"
     CDS             complement(3125..4219)
                     /locus_tag="PK710_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOT09528.1"
                     /translation="MTQYDRHQTLTRRQLVGAAAATALVGVAARARGQTPATSADRAE
                     KKDDPPSLAARRPDGFIPMAAPGLIVKVSKSNTLQPNQLWPTEEAAKVMLERAMTEFT
                     GKNDLGQAFGRFVHPQDKVAIKVNGIAAQKGATMGTNKELILEIVRGVLAAGVKPENL
                     WVFEQYPSFLAGTRIKDDVLPEGVKSYTHNNTDAGTGQIRIHAIRTRFARQLMEATAV
                     INVALIKDHSLCGYTGTMKNMTHGVTLNPHDFHANLMDPQIPLVYAQDAIRSRVRLQI
                     TDGFKLIYNAGPLDRDPRARVPHEAVYVSTDPVAMDRIGWQIVDKWRVDRGLPTLEKA
                     KREPTYIQRAADMGLGIADLNRIRMKEIDL"
     gene            complement(4268..5152)
                     /locus_tag="PK710_07155"
     CDS             complement(4268..5152)
                     /locus_tag="PK710_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOT09529.1"
                     /translation="MAHLLVVSLIWAFSFGLIKGNLQGLDPSFVSWVRMTLSLLVFLP
                     FLRPAQMKWRQAWQLIVLGGVQYGLMYVFYIASFETLKAHQVALSTVLTPLFVVGLEA
                     WTTRTLRARFALAALLAVGGAALLNPDPTCLGAWKGMLLVQGSNLCFAVGQVWYRRLG
                     RGPTPDRSSFALAYAGAVLVTGMAALLTNDSIPSSLTFTQIWTLGYLGFVASGICFFL
                     WNVGAKATNAGVLAVFNNVKIPLAVLVSLLVFGESAQWSRLLSASAMIALALWLGART
                     SKQASPEKALASVRNDTK"
     gene            5301..6455
                     /locus_tag="PK710_07160"
     CDS             5301..6455
                     /locus_tag="PK710_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT09530.1"
                     /translation="MNTPYLSMGVRASWLAWLVLTLLLVGCGKGGLQLTHIRSAYKRP
                     SNVAVYFKVQMSDGQPVGGLTADQFVVYEDGKVVSQFESQQTIINPEAAASHYTLLLV
                     DMSGSISESGESGAVVEAATAFTERVEKYQKVGVYAFDGSEDLHPVAPFTNSAGSATA
                     GVKRLATFKAKDPSTNLHGAIVKGLEELDKALQGAEHPMRFGTLVVFSDGTDRAARVP
                     ASEMRDAIKNTSFDVFAIGLGAEISEDELKKVGKNGTAMAADRQAVVQAFDTIAKRIE
                     DLTKSYYLLSYCSPSRAGEHEVRIEAVVKQGDKERKGSVTARFDAQGFGPGCNPQQPP
                     DFDIKSGDAVAEGEEAKPKGTGARVKPNAGAGASGGPSKGMGAAGGDFNP"
     gene            6890..7159
                     /locus_tag="PK710_07165"
     CDS             6890..7159
                     /locus_tag="PK710_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09531.1"
                     /translation="MDTKNLKVVYTITERGERSYWHRIGVGYVNRDGSLNIKLDAVPV
                     NGTMHVRDWTARDEVTAQEPKKSNGSNGASKNASSKLIDDLPGTF"
     gene            7302..7844
                     /locus_tag="PK710_07170"
     CDS             7302..7844
                     /locus_tag="PK710_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOT09532.1"
                     /translation="MAMTIQKRVEHAGWMGRVHRLVNGPWTGPVAKAGAVVGAVVLLS
                     WLGMRSAQSSVSPSGQLDPEMMEVMKASLVAGGGEASAAPMGSVAGDAGASPGRAVGV
                     LPDGRIVLNAASAEELCKLPGIGPARAAKIVELRGKLGGFRSVRQLLRVKGIGPRMLK
                     RLTPLVVLDAPVQGESRDGG"
     gene            complement(7853..8209)
                     /locus_tag="PK710_07175"
     CDS             complement(7853..8209)
                     /locus_tag="PK710_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09533.1"
                     /translation="MKKTPLQIVKERFGNKAALIEALQKFTHEDLWVNRLNSEKGLEH
                     VSNAKLLRLHDTFSAVKEQFGNRFKLIDAILEAENRSKDTGYRERLEKYPVPRLFDLY
                     RSVTRRKNREDKAKKD"
     gene            8394..9314
                     /locus_tag="PK710_07180"
     CDS             8394..9314
                     /locus_tag="PK710_07180"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOT09534.1"
                     /translation="MQTRISLWLLWICVLLAFSVVATVGCGGASYSRSRGDYAVPASA
                     MPNEAQEQASSGASDSDDTYGYAFDGRVMAEPIAEYVSGGSSLEADKKGTAQVVPKPT
                     TSDSAAADSKPSSSKGESAVASDAVPIQQVLIYTAELTMAVYQVDKSMSEVEAIARNL
                     GGFLATRTDTRITIRVPAAQFDDALKGIGGMGDVLSRNVQVDDVTEEYLDVLLRLKNA
                     RQVRERIAQLLANAKTVEESLQVERELHRLSAEIERMEGRLKYLRDKARYSTITVTFR
                     PLQADVVGRPFQLPFPWLRNLGLGGLMDVQ"
     gene            9331..9810
                     /locus_tag="PK710_07185"
     CDS             9331..9810
                     /locus_tag="PK710_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HOT09535.1"
                     /translation="MTNLSVRMRAWLGLMLLGIVGCGSTTFYAATPPGFVELPDQEPG
                     YQYRAVSADGVVVGVRSIEHEPKGDKEFWVQAIRNRMRDRGGYALLSNQPVKTKSGLE
                     GTQLKFGHDEKGQSMLYTITLFVTPDYLFLVEFGGTEEEMTRQAAYLNWIIENFRRT"
     gene            10000..11436
                     /gene="gltX"
                     /locus_tag="PK710_07190"
     CDS             10000..11436
                     /gene="gltX"
                     /locus_tag="PK710_07190"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOT09536.1"
                     /translation="MGEFAPSPTGYLHIGGVRTALFNWLWARKMGGSFVLRIEDTDQE
                     RSTPESVKVILDSLRWLGLDWDEGPEVGGAHGPYFQMQRLSLYEKYAQELIDKGKAYR
                     CYCTKEELAAARESLRKDNPKAQFRYPGTCRHRKDRPDAPYVVRFNMEAVQREAVTYR
                     DLVFGEITTPNREQQDFVLLRSDGVPLYNFGVVIDDLTMGIDLVMRGRDHMVNTPPQI
                     LLYEALGVKPPTFAHLPMMLSPKGEKLSKRHGAVSVSEYEKMGYTPGAVLNYLARFGW
                     SCGDQEIFSLEELVDKFSFESLNRSDGRFDAKKFADVAFEHLKEPRLTSDADYSRHLH
                     PFMAARGLGSVDEAMLRKAIGTVRARGRTLADAAERIEFYFRPVVFDDKAKEKFLTPD
                     VVEPLQAMIACLSSLDSFDEGEIEQAIQQLLASRGWEMKEIAQPARVSLTGQKVSPGV
                     FEMLAVLGREESIRRLRQGVQIARGTAS"
     gene            11433..12398
                     /locus_tag="PK710_07195"
     CDS             11433..12398
                     /locus_tag="PK710_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOT09537.1"
                     /translation="MSSCSGLMALAAPPWVDAPSALHQWLESPLVKAPLPVPVFFLLA
                     PLMWLLFRGTWKQLDVEAQKERGHRLAMGKWDARPAVACVIMAVVLTLQEYYGGGRTY
                     QSVIRPFLMDQQDKWLSGVIDMAKYDNLYSYAWWSFARVLGYVLIPLPLWKILFPRDS
                     LLDMGFRVSGFFRHVWIYGVSLAVVIPAMFLVARQPDFGTYYPFYKLASRSWFDLMAW
                     EVMYFAQFLALEIFFRGWFLGALRRTVGAAAIFVMAMPYCMIHYGKPYLEAHGAIIAG
                     IFLGSLSMKTRSIYAGFLVHITVALSMDLLSLFNRDALPTKWMAP"
     gene            12469..>13029
                     /locus_tag="PK710_07200"
     CDS             12469..>13029
                     /locus_tag="PK710_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOT09538.1"
                     /translation="MAYHLLDEPLVETLRSSGFHGSFTMQRHPSFPVSSLFAGAIVLL
                     ASAASADIYTFTDDDGVIHFTSKPQPGSSLYLKAPRRRPQPGVVPVMPSDTSLERFTR
                     YDTAIVQAATLYQIPVELVRAVIKVESDFDPRAVSPAGARGLMQMIPATAERMQVRDI
                     FDPRENIFGGVRYLRVLANMFNGDLQL"
BASE COUNT         2582 a         3148 c         4345 g         2954 t
ORIGIN      
        1 gcttgaattg ggccgttccg accacccgtt tcgaaactgc gacaaggcga ggttgacggg
       61 ggttcgaagg gcgctcgagc aagtattcgt tggcgaaggg tggaatccat ggccgatgtc
      121 ggtgctgcga ggcaggcaaa ggccaacgat gatgtgatta tcttcgtggg agggattcac
      181 aggggcatgg gggctcgagc ggaaagatgc ttcggcgagg ggatcaggcc gtggtggcga
      241 gcacgatatt ttgggcagac ggttcttgtt cgagcgcatc gaggacctca tcggctgggc
      301 accggccttt ttcgaggagt atttcgagat ccttcaggta gatatcttct gcctgtcccg
      361 cggaatcacg ctggttgcgt cgtccgagac cttctttcgc gatcgacaac acgcgctggg
      421 cccaggtggc gacggtcttt ccgtggaaca tggcgttgag tccttcgttt ccgagggatt
      481 ggcgcaaggt gaccatggaa ggataggtcc aggtttcggt gagccgttcg gcggcgtcca
      541 aggccttatc gtcataaagg atgccggtcc acagggcggg gagggctggt gtatatcgat
      601 tggggaccga gtcggcgcca cggacctcga tggtttgctt gagtcggact tcggggaaga
      661 gagtattcag gtgagcggtc caatcctcga gggtggggcg atatccctgg aaaccgtcga
      721 tcaaaaagct gcgaaaggtt tgtcctgtat tttcaatggc tttgccttgt ctgaggaaga
      781 ggaacatggg ggcgtcgagg gcccattgga tataatcatc caaggtggcc gacgggttcc
      841 acatttgggg cagcagtcct tgcctggcgg ggtcgacctg gagccaggcc tggatacgac
      901 ggctttttcc gccgtagcgg cggccttcga cgaacggacc gttggcgaac atggcggaga
      961 cgatggggga gagacggagg ctaacgcgca acttccgcaa tccgtcttcg atgctcgaaa
     1021 agtcgaaatt ggcttgtacc gtagcggttc gtcgcatcat ttcgagggca tcgccgcctt
     1081 gggaggcgag atacacgcgc atgattccgt aacggagttt gggcacccag ggtagttggt
     1141 cgtgtctggc gagaggatgg aagcccgagg caagccaggt gatgcccatg ggatcactga
     1201 tggattgcaa ctcggtattg tacgtttgac gctcattttc gagggcgtgc aagtcatgcc
     1261 atggggagcc cgaaaactcg aattgtcccg cgggttcgag ggtgatggcg gcgccgtttc
     1321 gtttcaacga caccactggg cccccggggc tttcgctttt ggctagccaa ccgtatcggg
     1381 ataccaattc atggaacaaa ccgaggatgc cgtcctggcc atcgtattgc agcggctgca
     1441 aggtgcgtga gtacacgccg aagcgctcgg cttcgatgcc gattcgaaag gcgtgtcggg
     1501 gcttgcaagc agaggaaaag ggccacgaca attggtcgaa gtcgagaagt tcactaccgg
     1561 attcggtttt cggtcttgga tgagcaggat gaagattcat cgtctcccat tggatgatgc
     1621 aattggtgat ggcgtagcgg gcgaagcgac ggttcgctcg cggatttgca attacttgcg
     1681 ttcaataccg atgagaaagg tctctacgct acgacggcga gtgccttctg gacggatgat
     1741 acggactttg ttgaatcgtt ctcgcaggtg ctggcgtgcc tgttcgaagg ttgcacccat
     1801 gaatagtttt cccacgaaag cacttcctcg acagccgagg gaggccgcga catcgacggc
     1861 gcgcatgaac aagtccgcgg agcggatttc gtcgacgtgg ctgaatcctg ttgtgttggg
     1921 ggccatatcg gagagcacga catcataggg ggcgaaggta gcgagggcgg gttcttgtag
     1981 cgcgagagca tctccctgaa ggaaggtgac gttggaaccg agggggtagt cgatgggttg
     2041 caagtcgatg gcgaggattc gtccgatggg gccgacgcgt tgtgaggcgt acatggacca
     2101 ggagcctggg gcggcaccga ggtcgagcac acgcatggcg gggcgcagca attggacgcg
     2161 cctatcgatt tcttcgagtt tgaagatgct tcgggcggga tatccctgtt cgcgcgcttg
     2221 gcggctccgg tgatcgggac gtcgataggg gttacgagcc acccggatta cttggctttc
     2281 gggtgtccac ccattttctt gacggcgcca cggatcacga cgattccgtt tcggctaccg
     2341 ggcacgccgc cgtggaccag gagcaggtct tgttccggga tgatcttggc gactttgagg
     2401 ttgagcacgg agcattgttt gttgccgtat tggccgggca ttttgacgcc tttgagggtt
     2461 cggccggggg tcaagttcgt gccgatggat ccgccgtggc gacggtattc atgcgtgccg
     2521 tgggtgttga cctgtccttt gaagttgtaa cggcgcatga cgccggagaa gccatggccg
     2581 cgggatttcg cgcggacatc gatgaattgg ccgacttcga agaccttatc gagttcgagt
     2641 ttctggccga tttcgtattg tgcgaccacg tcggcgggca ggcggaattc cttgaggatg
     2701 cgtttgggcg tgacgcccgc ttttttgaaa aagccgccga gcggtttgtt ggtatgcttg
     2761 ggttttcgct cgtcgagacc cacgatcaaa gcgctgtagc ggtctttgtc cagggtgcgt
     2821 tttccgacga cgacggcggc ggcttcgatg acggtgacgc gctggacgat gccgttatca
     2881 tcgaagaatt gagtcatgcc gagctttcgc ccgatcgttc ctgggtgttg gttcatgata
     2941 caccataagc tccgtcaaag agtgatgatg aagggcggga aatccgccgg tccgaccgtt
     3001 tctcccgatg gggctggtgc cggagcggct tacaccaggg agcacggggc tccagagcgg
     3061 aagggacgcg cagcataaac ggattgggcg gctcgtcaag gcaagaagca gcgattgcgg
     3121 tgcgttacaa gtcgatttct ttcattcgaa tccggttcaa atcggcgatg cccaggccca
     3181 tatccgcggc tcgctggata taggtgggtt cgcgtttcgc tttttccaag gtcggaagcc
     3241 cgcggtccac gcgccatttg tcgacgattt gccatccgat gcggtccatg gcgaccggat
     3301 cggtgcttac gtacacggct tcgtgaggca ctcgcgcgcg agggtcccga tccagggggc
     3361 cggcattgta aatgagcttg aaaccatcgg tgatctgcaa gcgaacccgg cttcgaatcg
     3421 cgtcctgggc gtagaccaag gggatctgtg gatccatcaa attggcgtga aaatcatgag
     3481 ggttgagcgt aaccccatgc gtcatgttct tcatggtccc cgtgtatccg cacagggaat
     3541 ggtctttgat caacgcgaca ttgatgactg ccgtggcctc catgagctgc cgtgcaaaac
     3601 gcgttcgaat cgcatgaatt cgaatctgac cggttcccgc gtcggtgttg ttgtgcgtat
     3661 agcttttaac cccctcgggc aggacgtcgt ctttgattcg agtacccgcg agaaaagatg
     3721 ggtattgctc gaatacccat aaattttcgg gtttgacacc cgccgcgagc accccgcgga
     3781 cgatctcgag gatcagctct ttgttggtcc ccatcgtggc acctttttgc gcggcgatgc
     3841 cgttgacttt gatggccacc ttgtcctggg gatgaacgaa gcgcccgaac gcctgtccaa
     3901 ggtcattttt ccctgtgaat tctgtcatcg ctcgctccag catgaccttc gccgcctctt
     3961 ccgtcggcca caactggttg ggttggagcg tattggattt ggatactttg acgatcaacc
     4021 ccggtgctgc catcggaatg aaaccatcgg ggcgtcgtgc ggccaaactc ggaggatcat
     4081 cttttttttc cgcacggtcc gcggatgtgg caggagtttg ccctctcgct cgggccgcca
     4141 cgccaacaag cgccgtggct gccgcggctc caacgagttg cctccgagtc agggtttggt
     4201 ggcgatcgta ctgcgtcatc gtcttcctcg ttcacgaacc atgggtgaat tcgcgttgtt
     4261 tcgccagcta tttggtatcg tttcgcaccg acgccagtgc cttttcgggc gaggcttgtt
     4321 ttgaggtccg tgcgcccagc cacagggcca gcgcgatcat ggcgctggcc gacagcagcc
     4381 gggaccattg cgcggattcc ccgaaaacaa gcagagaaac cagcacggcg agggggattt
     4441 tcacgttgtt gaacaccgcc aacacgcccg cattcgtggc ttttgctccc acgttccaca
     4501 gaaaaaagca gatcccagag gctacgaacc ccagataccc aagtgtccag atctgggtga
     4561 acgtcaggct cgacgggatg gaatcgttcg tcagcagggc ggccatgccc gttacgagca
     4621 cggcgcccgc gtaagcaagg gcaaaactcg atcggtccgg agtcggtccc cgtcccaagc
     4681 gtcggtacca tacttgtccg acggcgaaac agaggttgga gccctgcacg agcagcatgc
     4741 ctttccacgc gccaaggcaa gtggggtcgg ggttgagcaa ggccgcgcca ccgactgcca
     4801 aaagggcagc gagtgcgaaa cgagcacgaa gcgtacgcgt tgtccaggcc tccaagccca
     4861 cgacgaaaag cggagtcaag acggtggata gcgcgacttg atgggctttg agggtttcga
     4921 aggaggcgat atagaagaca tacatcaagc cgtattgcac gccgccaagg acgatgagtt
     4981 gccaggcttg gcgccatttc atctgggcag gacgcaaaaa aggcaaaaaa acaaggagcg
     5041 aaagtgtcat ccgcacccaa gacacgaagc ttggatcgag gccttgtaag ttgcctttga
     5101 tgagaccaaa ggaaaaggcc cagatgagcg aaacgacgag taggtgtgcc atgggtggat
     5161 gaaacggggc agcgtcttat ccaacaacgc ggtgttcgaa caagggcgcg atgacacgat
     5221 agcgtgtcga accgcaataa cgccaggaac ctcgtgcgac gcgcttgtgc aatcttgcct
     5281 ttggtcttta caatccacgt gtgaacactc cgtacctatc gatgggcgta agagcttcgt
     5341 ggctcgcttg gttggtcctg acgctgctgc ttgtgggatg cggaaagggc gggttgcagt
     5401 tgacgcatat tcgcagcgcc tacaagcgtc cgagcaatgt cgccgtctac ttcaaggtgc
     5461 aaatgtccga tggtcaaccg gtggggggac tgacggccga tcagtttgtg gtttatgagg
     5521 atgggaaagt cgtttcccaa ttcgagagtc agcagaccat catcaatccc gaggcagcgg
     5581 cttcccatta cacgctgctt ctcgtggata tgagcggtag catctccgaa tccggtgagt
     5641 ccggcgctgt ggtggaggcg gcgactgcgt ttaccgaacg ggtggagaag tatcagaaag
     5701 ttggtgtgta tgccttcgat gggagcgagg atttgcatcc ggtggctccg tttacgaatt
     5761 cggcgggcag tgcgaccgcg ggtgtcaagc ggctcgctac cttcaaagcc aaagatcctt
     5821 ccaccaattt gcacggcgcg atcgtcaaag ggctcgaaga gttggacaaa gctcttcaag
     5881 gggcggagca tccgatgcgt tttgggacac tcgtggtctt ttcggacggc acggatcgcg
     5941 cggcacgcgt acccgcgagt gaaatgcgcg acgcgatcaa aaacacttcc ttcgatgtgt
     6001 ttgcgatcgg gcttggggct gagatcagtg aagacgagtt gaagaaggtg ggaaaaaatg
     6061 gcacggccat ggccgcggat cgtcaagccg tcgtgcaagc attcgatacg attgccaaac
     6121 gcatcgagga tctcaccaag agctattatt tgttgagcta ttgctctcct tctcgggcgg
     6181 gggagcacga ggtgcgcatc gaagcggtgg tcaaacaggg agataaagaa cgaaagggca
     6241 gcgtgaccgc gaggttcgac gcccaaggat ttggtccggg atgcaatccg cagcaaccgc
     6301 ccgatttcga tatcaagagc ggagatgcgg tggcagaggg tgaagaggca aagcccaagg
     6361 gcacgggggc tcgcgtcaag ccgaatgcgg gggcaggcgc atcgggcgga ccgagcaaag
     6421 ggatgggagc ggcaggggga gatttcaatc cctgaatacg atacgccttc cctgggtcat
     6481 tcacgcgaac cgatgtcgct ttcgaaaagc gaaaacgtcg ccacgatgac atcacgcgcc
     6541 gtaggatacc gggctgcttg aagcggcctt ctgacgaagt ttgagcgttg acaggcgatg
     6601 ggtcgtcgtc gtttcgatgg tacgggaacc ggtggcgacc gccaattcgg ggtggcgctt
     6661 cgccacttgc gatctatcta tttgtaattt cacgtatttt ttagggacca agggcggatt
     6721 cgggcgtgga agccgtggcg gccgccactt gaggaataat tataaaaatc aataaattca
     6781 ataggataga tccttttcgg aattggcccg ttcttcgcaa tctcgatgcc gcaagaaggt
     6841 tccgaagccg agcgaggtgc ttggcgatag ggggaccgcg gaggaaagca tggatacgaa
     6901 aaacttgaag gttgtgtaca ccattacgga gcgtggagag cggagctact ggcatcggat
     6961 tggggttggg tatgtcaacc gggacggctc gttgaacatc aagttggatg cggtgccggt
     7021 gaatgggacg atgcacgtgc gggattggac ggcgcgggac gaggtgacgg cgcaggagcc
     7081 gaagaaatcc aacgggtcga atggggcttc gaagaacgcc tcctcgaagc tcatcgatga
     7141 tcttcccggg acgttctagc gatcggaatc cctacgtttg cggctaacga ggtgcgcttc
     7201 gcgtggagca tggattgcgc gaagcgcctt cgtttttggg gttcgatgga agggtggcga
     7261 gggtttcgtg gtgttgccac gacgaacaaa ggagtgagcg gatggcaatg acgatacaaa
     7321 agcgggttga gcacgcgggt tggatgggac gggttcaccg gctggtgaat gggccgtgga
     7381 cgggtccggt ggcgaaggcg ggagcggtgg tgggggcggt ggttttgctg tcctggctgg
     7441 ggatgcgaag cgcgcaatcg tcggtgtcgc catcgggaca attggatccg gagatgatgg
     7501 aggtcatgaa ggcgtcgttg gtcgcggggg gaggcgaggc gagtgcggcg ccgatggggt
     7561 cggtggcggg tgatgcgggg gcgtcgccgg gacgtgcggt gggggtgttg ccggatgggc
     7621 gcatcgtgtt gaacgcggca tcggcggagg aattgtgcaa gctacctggg attgggcctg
     7681 cgcgagcggc gaagatcgtg gagttgcgag gcaagcttgg tggatttcgg tcggtacgcc
     7741 agctacttcg cgtcaagggc attgggcccc ggatgctcaa gcgattgacc ccgctagttg
     7801 ttctggatgc gcctgtgcag ggggagagtc gggatggggg ataagcgcga ccttagtcct
     7861 tcttggcttt atcttccctg ttctttctgc gcgtaacgct tcggtacagg tcaaagagac
     7921 gagggacggg atacttttcg aggcgctcgc ggtatcccgt atccttgctg cggttttcgg
     7981 cttcgaggat ggcgtcgatg agcttgaaac gatttccgaa ctgctctttg acggcggaga
     8041 aggtgtcatg caagcgaagc agcttggcat tggacacgtg ttcgaggccc ttttcggagt
     8101 tgagtcggtt gacccagagg tcttcgtggg tgaatttttg cagggcttcg atgagggcag
     8161 ctttgttgcc gaagcgttcc ttgacgattt gcagaggtgt ttttttcacg atgcgtcctt
     8221 ccggtggtgc gtgagatgac tcacgcggtc attgaagtgc agcggtctag cagaaccgtg
     8281 agtgataggg aatggggaac cgacgggatg gcaatcccga agcctttgcg gaaaagatcg
     8341 tgtaagctcg ggcctttcgc gggcgttaat cccgaaaacc aagggagagc ttcatgcaaa
     8401 cgagaatttc gctatggctt ctgtggattt gtgtcttact tgcgttctcg gtggtagcga
     8461 ccgtggggtg tggtggcgcg agctattcga gatcccgggg ggattatgca gttccagcgt
     8521 ctgcgatgcc gaacgaagcc caggaacaag cttcgtcggg ggcttccgac tctgatgata
     8581 cgtatgggta tgcttttgac ggaagggtga tggcagagcc tatcgcggag tatgtgtcgg
     8641 ggggcagttc gctggaagct gacaaaaaag ggactgccca ggtggtgccg aaaccgacca
     8701 cttccgacag cgctgcagcg gactcgaaac cgtcttcgtc gaagggtgaa tcggctgtgg
     8761 caagcgacgc ggtgccgatt cagcaggttc tcatttacac ggcggaactg acgatggcgg
     8821 tgtatcaagt ggataagtcg atgtccgagg tggaggcgat cgcgcgcaat ctcggtggat
     8881 ttcttgcgac tcgtacggac actcgaatca cgattcgcgt tccggcagcg caattcgacg
     8941 atgcgttgaa ggggattgga ggaatggggg atgtattgtc gcgcaatgtg caagtcgacg
     9001 atgtaacgga ggaatatttg gatgttctgc ttcggctgaa aaacgcaagg caggttcgcg
     9061 agcgtatcgc ccagcttttg gcgaatgcca agacggtcga ggagtcattg caagtggagc
     9121 gagaactgca tcgcttgagc gcggagatcg agcgtatgga aggcaggttg aagtatcttc
     9181 gagacaaggc gcgatactcg acgataacgg tgacatttcg gccgctgcag gcggacgttg
     9241 tggggcgtcc gtttcaactt ccctttccgt ggcttcggaa tctggggctg ggtgggctga
     9301 tggatgttca gtgatggatg gagggagacg atgacgaacc ttagcgtacg aatgcgagct
     9361 tggttggggc tgatgctgtt ggggatcgta gggtgtggga gcaccacgtt ttatgcggca
     9421 acaccccctg gttttgtcga gttgccggat caagagcctg gctatcaata ccgtgcggtg
     9481 agtgcggatg gggtggtggt cggggtgcgg tcgatcgagc atgaaccgaa aggggacaag
     9541 gagttttggg tgcaggcgat tcgcaatcga atgcgcgaca gaggcggtta tgcgctgctt
     9601 tccaaccaac cggtgaagac caaaagtggg ctcgagggca cgcagcttaa gttcgggcat
     9661 gacgaaaaag ggcagtcgat gctgtatacg atcacgctgt tcgtgactcc ggactatcta
     9721 tttctcgtgg aatttggtgg gacggaggag gaaatgacgc gtcaggcggc ctatttgaac
     9781 tggatcatcg agaattttcg aaggacttga ggagcgcaga ggcgcctcgc cgtgcggctc
     9841 gcgtgacagc gtagggggcg gatttcgctt gatatcgtaa gaattgtcgc atgacgtgac
     9901 cttgggcgca tcgcgtggta tctgcccgcg ccatggccaa taatcctgct gcttctcctc
     9961 gcgttcgctt cgcaccttct cccacgggct atctgcatat tgggggagtt cgcaccttct
    10021 cccacgggct atctgcatat tgggggagtt cgcacggcgt tattcaattg gttatgggct
    10081 cgcaagatgg ggggctcgtt tgtgttgcgc atcgaggaca ccgatcagga gcgcagcacc
    10141 cccgagagtg tgaaagtcat ccttgattcc ctccgatggc ttggtctcga ttgggacgaa
    10201 gggcctgagg tgggaggcgc ccatggtccg tactttcaga tgcagcgtct ttcgctttac
    10261 gaaaaatacg cccaggaatt gatcgacaag ggcaaagcct atcgttgtta ttgcacgaaa
    10321 gaggaacttg cggccgcgcg cgaatcgttg cgaaaggaca acccgaaagc gcagttccga
    10381 tatccgggca cgtgtcgtca tcgcaaggac cggcccgatg cgccgtatgt cgttcgattc
    10441 aacatggagg cggtgcaacg ggaggcggtg acgtaccggg atttggtttt tggtgagatc
    10501 acgacgccga accgtgagca gcaggatttc gtgttgcttc gatccgatgg ggtgccgcta
    10561 tacaattttg gggttgtgat cgacgatttg acgatgggca tcgatttggt gatgcgaggt
    10621 cgggatcata tggtgaacac gccgcctcag attctgttgt acgaggcgct tggggtgaag
    10681 ccaccgacgt ttgcgcatct tccgatgatg ctatcgccca agggagaaaa actaagcaaa
    10741 aggcatggcg cggtgagcgt gagtgaatac gagaaaatgg gatatacgcc gggagccgtg
    10801 ctcaattatc tggcgcgttt tggttggtcc tgtggtgacc aggaaatttt ttctctcgag
    10861 gagttggtgg acaagttcag cttcgagagt ctgaaccggt cggatggtcg tttcgacgcc
    10921 aaaaaatttg cggatgtggc gtttgagcac ttgaaagagc cccggctcac ctctgacgcc
    10981 gattacagtc gccatttgca tccgttcatg gccgcacggg gcttgggttc ggtggacgag
    11041 gcgatgcttc gcaaggcgat tggtacggtg cgagcgcgag ggcgaacgct tgcggacgct
    11101 gcggagcgaa tcgagtttta ttttcgcccc gtggtattcg atgacaaggc gaaggaaaaa
    11161 tttctcacac cggacgtggt cgagccgctc caagcgatga tcgcgtgttt gtcgtcgttg
    11221 gattcgttcg acgaagggga aatcgagcaa gcgattcagc agctattggc gagtcgggga
    11281 tgggagatga aggagattgc gcaaccggcc cgagtatccc taacgggaca gaaggtgagt
    11341 ccgggagtat ttgagatgct cgcggtattg gggcgtgagg aatcgattcg acggttgcgg
    11401 cagggggtgc agattgcgcg aggcacggca tcgtgagttc gtgcagtggg ttgatggcgc
    11461 tggcggcgcc gccgtgggtg gacgctccga gtgctttgca tcaatggctc gagtcaccgt
    11521 tggtcaaggc accgctcccc gttcccgtat ttttcctttt ggcgcctttg atgtggctgt
    11581 tgtttcgcgg gacgtggaaa cagctcgatg tcgaagcgca aaaggaaagg gggcatcggc
    11641 tagccatggg caagtgggac gcgcgtccgg cggtggcttg cgtcatcatg gcggtcgttt
    11701 tgaccctgca ggagtactac ggtgggggac gaacgtacca atcggtcatt cggccgtttt
    11761 tgatggacca gcaggataag tggttgtcgg gcgtcatcga tatggccaag tacgacaact
    11821 tatatagcta cgcgtggtgg tcttttgcgc gcgtgttggg ctacgtgctc atccctcttc
    11881 cgctttggaa aatcctgttt ccccgggaca gtttgctgga catggggttt cgtgtttcgg
    11941 gattctttcg ccacgtgtgg atttacgggg tgtctttggc ggtggtgatt ccggccatgt
    12001 ttttggtggc tcggcagccg gatttcggca cctattatcc gttttacaag ctggcgtcgc
    12061 ggtcctggtt cgacctgatg gcttgggaag tgatgtattt cgcccagttt ttggctctcg
    12121 aaatcttttt tcggggttgg tttctcggag cgcttcgccg gaccgtggga gccgcggcca
    12181 tttttgtcat ggcgatgccc tattgcatga ttcactacgg caagccgtat ctggaggcgc
    12241 atggggcgat catcgccgga atatttcttg gttccctgtc gatgaaaaca cgaagtattt
    12301 acgcgggatt tctcgtgcat atcaccgtag ccttgtcgat ggatctattg tccttgttca
    12361 atcgcgatgc gttgcccacc aagtggatgg cgccgtgagc gggaatgatc gtgtgggctc
    12421 gacggttatg agccgggtgt agtcaagagt ttgcgtactg gctcggtcat ggcctaccat
    12481 ttgctggacg agcctttggt tgaaacgctg cggtccagtg gttttcacgg ttccttcacg
    12541 atgcaacgac acccctcttt ccccgtgagt tcgctgtttg ctggggccat cgtgcttctg
    12601 gcgagtgccg catcggcgga tatctataca tttacggatg acgatggggt cattcatttt
    12661 accagcaaac cgcagccagg ttcctcgctg tatctcaaag ctccacgtcg tcgtccgcaa
    12721 ccaggggttg tgcccgtgat gccgagcgac acgtccctcg agcggtttac tcgttatgac
    12781 acggcgattg tgcaggcggc gacgctttac cagattccgg tggagttggt gcgtgcggtg
    12841 atcaaggtgg agagtgattt cgatccgcga gcggtatctc ccgcgggggc tcgaggattg
    12901 atgcagatga tcccggcgac ggcggagcgg atgcaggtgc gcgatatttt cgacccgcgc
    12961 gagaatattt ttggtggtgt gcggtatctt cgagtgctgg ccaatatgtt caatggggat
    13021 ttgcagcta
//
LOCUS       DAOSOR010000094        12953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4829_length_12953_cov_5.242906, whole genome shotgun sequence.
ACCESSION   DAOSOR010000094 DAOSOR010000000
VERSION     DAOSOR010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 12953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12953
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4829_length_12953_cov_5.242906"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(388..4662)
                     /gene="rpoC"
                     /locus_tag="PK710_07205"
     CDS             complement(388..4662)
                     /gene="rpoC"
                     /locus_tag="PK710_07205"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOT09539.1"
                     /translation="MRDIFSFFEKPKDPLSFSAIRISLASPEKIREWSHGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPVKDYECNCGKYKRMKHRGIVCEKCGVEVIQSKVRRERL
                     GHINLATPVAHIWFLKSLPSRIGNILDITLKDLEKVLYCEAYVVIDPKETDLEVGQLL
                     NEDKYLQKLDDYGDEAFTAAMGGEAVLEMLRRVDVHQLAEDLRAEMRESTSEAKRKKI
                     AKRLKVVEAFRDSGNRPDWMMLTVIPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKRLLELNAPEIIIRNERRMLQEAVDALFDNGRRGKTITGPNKRPLKSLSDMLKG
                     KQGRFRQNLLGKRVDYSGRSVIVVGPTLRLHQCGLPKKMALELFKPFIYNKLEERGYV
                     NTIKSAKKMVEKERPEVWDILEEVITEHPVMLNRAPTLHRLGIQAFEPTLIEGKAIQL
                     HPLVCTAFNADFDGDQMAVHVPLSTEAQMEARVLMMSTNNILSPASGRPIINPTQDIV
                     LGLYYATRARKFARGCYREVQLLDGEGKLTGKPLRGTFASVEEVRMAYDHGEVDLHAR
                     ISVRVPDLEDDGTPRLDALGRPKRHLVQTTVGRVLISEILPKRVPFDFVNKTLDKKAL
                     SALIDKCYREHRNKETVLLADRLRSLGFNYSTYAGVSICMDDMVIPEKKAVLLTEAQK
                     RVEEVVEQYQDGLITDGERYNKIVDIWASVADEVTREMMKGISSDAVVDPETGEQSDE
                     PSFNPIYIMADSGARGSTQQIRQLAAMRGLMAKPSGEIIETPITANFREGLTVLQYFI
                     STHGARKGLADTALKTANSGYLTRRLVDVGQDCTIQEYDCGTLDGIRVTKLEEAGEVI
                     QPLADRILGRVVLEDVIDPLTGEVLVEANTELEETLVKTIEDAGIEEVVIRSGLTCEA
                     RRGVCAKCYGRDLARGYRVNIGESVGIIAAQSIGEPGTQLTMRTFHIGGAAARGKIEQ
                     SSLEARTEGFVKTRGAHLVTRSDGVTVVMNRHGELVVRDETGREREHYRLQYGAIMRK
                     KDGDAVKPGDIIAEWDPFATPIITEVAGNVVFGDLVEGVSVQEKVDEVTGLARKVVIE
                     SRDKELRPHISIRSVETGEILKLPDSELDATYFLPVGANIEQGLEDGIRVEAGMPVAK
                     IPRDTTKVQDITGGLPRVAELFEARKPKDHAVISEIDGEISFGKDMKGKRRIIATPFG
                     PDGSLQPTQAREYLIPKGKHLQVLPGDRVRAGDPLMDGPANPHDILRVKGEKELAAWL
                     VNEIQQVYRLQGVTINDKHIEIIVRQMLRRVRIKEVGDTNFLVDEHVEKHVFEAENAR
                     VMSRGLRHAVAEPLLLGITKASLSTDSFISASSFQESTKVLTEAAISGKQDDLRGLKE
                     NVIMGRLIPAGTGLPPYRRLQIIVDPDSNGTSHLPASTTNSVLSGS"
     gene            complement(4674..8924)
                     /gene="rpoB"
                     /locus_tag="PK710_07210"
     CDS             complement(4674..8924)
                     /gene="rpoB"
                     /locus_tag="PK710_07210"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOT09540.1"
                     /translation="MASVVQTNFRIRKNLGRVKRIIQVPNLIDIQKTSYDKFLQATVP
                     PSERAEVGLQAVFRSVFPIRDFNQTSELVFVSYNLEKPKYDVDECRQRGMTYAAPIKV
                     TTQLMIYEPRDNGERDVRDIKEQEVYFGEIPLMTDTGTFIINGTERVVVSQLHRSPGV
                     FFDHDKGKTHSSGKLLYSARVIPYRGSWLDFEFDPKDIIYVRIDRRRKMHATVLLRAL
                     GYSTQELLNYFYSTEDIFLEKGGKFSKSIEYDLLPGQRSTRDIVVDGTVVVKKNTKFN
                     KKAIKLLKDAKIERIPLEYSELLEKVAAHDVINLETGEVILECNDSIKNAQIPSPTDP
                     QKTVTLGDLCEKPSGKKPATEETVRNAIAHFYKYLSSVGVHQFKILFIDGLNVGSYLR
                     DTLIADKVKTTEDAIMEIYRRLRPGDPPTLDTAKTLFNNLFFNPERYDLSRVGRLKLN
                     YKFYNNLPEEQRPTLDQCVLTQQDILETVRHLIELKNGRGSVDDIDHLGNRRVRAVGE
                     LMENQYRIGLVRMERAIKERMSMSPEIDTLMPHDLINAKPVSAVVKEYFGSSQLSQFM
                     DQTNPLSEVTHKRRLSALGPGGLTRERAGFEVRDVHATHYGRICPIETPEGPNIGLIA
                     SLSTFARVNAYGFVETPYRAVSSEGVTEDINWLSALEEEGKYIAQATANLTPDNKFKD
                     HLVSARFNGDFRMVPPENIQIMDVAPNQMVSVAAALVPFLEHDDANRALMGANMQRQA
                     VPLIATEAPIVGTGMELRVALDSGVCAVATRDGVVESVDASRIVVRAEEEGADYPDIY
                     HLMKYRRSNQSTCYNQKPAVRTGDRVKKGEVLADGPAVDCGELALGRNVLVAFMPWQG
                     FNFEDSILISERIVKEDIFTSVHIEEFECVARDTKLGKEDITRDIPNVGEEALKDLDE
                     SGIVRIGAEVKPGDILVGKITPKGETQLSPEEKLLRAIFGEKAGDVRDSSLRVPPGVS
                     GIVINARVFSRRGTEKDDRAREIESQEQARLEKTRDEEVQILRDSAFRQVRGLLIGSV
                     TDGKLVDEKGVVLLAKGDEITIERLQAIPRKYWGLIEVPSADLVRQKLRELDELISIR
                     EEHFRERIERLSRGDELPPGVIKLVKVYIAIKRKLQVGDKMAGRHGNKGVVSRILPEE
                     DMPYMQDGQPVDLVLNPLGVPSRMNVGQILETHLGWGARELGEQLQRMVHEKFSGEAI
                     RERLKRVYNGNDDLLAIIDRMNDDDAIRLARKLRRGVFFATPVFDGATEDDIKEALSL
                     AELPRSGQAILFDGRTGEAFDEDVTVGVMYMLKLHHLVDDKIHARSIGPYSLVTQQPL
                     GGKAQFGGQRLGEMEVWAMEAYGAAYALQEFLTVKSDDVMGRTRMYESIVKGEFQLEA
                     GLPESFNVLIKELQSLCLNVELIETGPERSAGERTAAE"
     gene            complement(9060..9443)
                     /gene="rplL"
                     /locus_tag="PK710_07215"
     CDS             complement(9060..9443)
                     /gene="rplL"
                     /locus_tag="PK710_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOT09541.1"
                     /translation="MADITTEQLIDHLSQMSVMDMANLVKTLEEKWGVSAAPVAVAGV
                     AGPAAAEAAPAEEKLEFDVELKDCGPNRINVIKAVREITGLGLKEAKELVEGAPKVVK
                     EAIPKAEAEEMKKKLEEAGAKVELK"
     gene            complement(9531..10058)
                     /gene="rplJ"
                     /locus_tag="PK710_07220"
     CDS             complement(9531..10058)
                     /gene="rplJ"
                     /locus_tag="PK710_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOT09542.1"
                     /translation="MNRAERTASINEIRERFDRMVSAVFVDFAGMTVEDVTKLREEFR
                     KVGVDYKVVKNSLVHHAVKDRPYAQAVEPLLKGMTGIAWSYEEPGIAAKVIKAFAKDN
                     QKLRIKGGVVESDVLSPASVENVLATMPGKDELRSMLLATFMAPMQSLVRQLGAPRQN
                     FAYLLDARKRQLEGG"
     gene            complement(10062..10766)
                     /gene="rplA"
                     /locus_tag="PK710_07225"
     CDS             complement(10062..10766)
                     /gene="rplA"
                     /locus_tag="PK710_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOT09543.1"
                     /translation="MRKVANKHRAALERVDRTKRHTLEEAVSLVKSAAYAKFDETVEL
                     AVRLGVNPKYADQMVRGAIVLPHGTGQKVRVLVFAKGDKEREARDAGADFAGSDELIQ
                     RVQEGFLDFDRVIATPDMMSSVGKLGRILGSRGLMPNPKVGTVTFDVDKAVREAKAGK
                     VQYRVEKAGIVHCRIGKLSFTNEALVENAKALIQALVRAKPSTAKGTYLKGIALSSTM
                     GPGIRVDTADAAEEVK"
     gene            complement(10861..11301)
                     /gene="rplK"
                     /locus_tag="PK710_07230"
     CDS             complement(10861..11301)
                     /gene="rplK"
                     /locus_tag="PK710_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOT09544.1"
                     /translation="MKKIVGLIKLQLPAGKANPSPPVGPALGQHGVNIMAFCKDFNAR
                     TQSQGDMIIPVLITVYADRSFSFIMKTPPASVLIKKAAGLSLQKKPGSGSKEPNKIKV
                     GTITTQQVREIAELKLKDLNTTNVESAMNSIAGTARSMGVKVQD"
     gene            complement(11356..11886)
                     /gene="nusG"
                     /locus_tag="PK710_07235"
     CDS             complement(11356..11886)
                     /gene="nusG"
                     /locus_tag="PK710_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOT09545.1"
                     /translation="MAKKWYVIQTYSGYEAKVKSGLQERIKQYEMTDSFGEILIPTET
                     VQDHRAGGKTRVRQKTSLPGYVFIEMEMNEKTWHLVKETNRVTGFIGNQNPTPVKAPE
                     IDGLRQGMVEGAMKPKPQVTFEEGDEVRVIDGAFANFSGTVEEVKPEKQKLRVKVSIF
                     GRATPVELEFSQVEKR"
     gene            complement(11901..12488)
                     /gene="secE"
                     /locus_tag="PK710_07240"
     CDS             complement(11901..12488)
                     /gene="secE"
                     /locus_tag="PK710_07240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOT09546.1"
                     /translation="MARVDADNKSERDDETTSESSESDREQVDALVDANVDEGEEKLV
                     VRVGEDVAQGLSPTQLGATRYVMSGFFAVGIALAYVFGRSVSSVWTKLASTQSVVDKV
                     GWITYVGEDARDTWGMVVGGLAAIAAFIYVRRREDTRAWVDEAAAELAKVTWPNKKEV
                     TTGTVVVVVASIIATVYFMLLDRFWGFVTDLVYRA"
     gene            complement(12566..12638)
                     /locus_tag="PK710_07245"
     tRNA            complement(12566..12638)
                     /locus_tag="PK710_07245"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12603..12605),aa:Trp,seq:cca)
     gene            complement(12680..12832)
                     /gene="rpmG"
                     /locus_tag="PK710_07250"
     CDS             complement(12680..12832)
                     /gene="rpmG"
                     /locus_tag="PK710_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOT09547.1"
                     /translation="MGDRLKVVLACVECAARNYRTTRVRETKPSLEMKKYCPTCKRHT
                     VHRETK"
     gene            complement(12841..>12953)
                     /locus_tag="PK710_07255"
     CDS             complement(12841..>12953)
                     /locus_tag="PK710_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09548.1"
                     /translation="MSIELITPVALEEQMRFAIREGGRTVGAGIVTEIVE"
BASE COUNT         2509 a         3611 c         3685 g         3148 t
ORIGIN      
        1 gttcgatcac gacggtggcc gatgaggcca agatggctag gatcaggaaa acatcgatcc
       61 acaggcccgt tcttggttgg gaagggtcgc cccgatatgg ctgaagaagc tcgtcgagcc
      121 gctgccggaa agtcagttgc gttttgggtt gggccatcgt agctcaggca aaggccgtga
      181 aatcgtacgc tgtgacaagc aggtacgtca acatcggcag gccagtgtgg aaagggagcc
      241 gcgatgaggg gaaagggagc gactcaggat cgtggcttcg gatcagtcca ctaagcccgt
      301 cgttcggaat gctccccacc cctatttccc cgggtttttt tgggaaaaaa ggggtgattg
      361 cggggatgat cggagtgaag tgatgcttca gcttccgctg agcactgagt tggtggtcga
      421 ggcgggtagg tgtgaggtgc cgttggagtc gggatcgacg atgatttgca gacggcgata
      481 gggaggcagt cccgtgcctg ccgggatgag gcgtcccatg atgacgtttt ctttcaggcc
      541 gcgcagatca tcttgttttc cactgatggc agcctctgtc agaaccttgg tgctctcctg
      601 gaagctcgag gcgctgatga atgaatcggt ggatagggat gccttggtga tgccgagcag
      661 caagggctca gcgacggcat gtcgcaagcc ccgagacatg acgcgggcgt tttcggcttc
      721 gaacacgtgt ttttcgacat gctcatcgac gaggaagttg gtgtcgccca cttccttgat
      781 gcgtacgcgt cgcagcattt ggcgcacgat gatctcgatg tgtttgtcat tgatggtcac
      841 gccttgcagg cggtagacct gctggatttc gttgaccaac caggcggcaa gttctttttc
      901 gcctttgaca cgaaggatat cgtgcgggtt ggcaggacca tccatcaatg gatcgccggc
      961 gcgaacgcgg tctccaggca gtacttgaag gtgcttgcct ttggggatca gatattcgcg
     1021 cgcctgggta ggttgcaggc ttccatcggg accgaaaggc gtggcgatga tgcgacgttt
     1081 tcctttcatg tccttgccga aagagatttc gccatcgatt tcgctgatga cggcgtggtc
     1141 tttgggtttg cgggcttcga atagttcggc cacacgcggc agaccgccgg tgatgtcctg
     1201 caccttggtg gtatcccgcg ggattttggc aaccggcata cccgcttcca cccgaatccc
     1261 gtcttccaaa ccctgctcga tgtttgcgcc caccggcaag aaataggtag cgtcgagttc
     1321 cgaatccgga agcttcagga tctctcccgt ttctacgctc ctgatggaga tatggggcct
     1381 gagttccttg tctcgcgatt cgatgacgac ctttcgcgcc aagccggtga cttcgtcgac
     1441 tttttcctga acgctgacgc cttccaccag gtcaccgaac acgacgtttc cagccacttc
     1501 ggtgatgatg ggtgtggcga agggatccca ttcggcgatg atatcgccgg gtttgaccgc
     1561 atcgccgtct tttttgcgca tgatcgcgcc gtattgcaga cggtaatgct cacgttcgcg
     1621 gcctgtctca tcacggacca cgagttctcc gtgtcgattc atgacgacgg tgacaccatc
     1681 gcttcgagtg acgaggtgag cccctcttgt tttgacgaag ccttcggttc tggcttcgag
     1741 ggagctttgc tcgatttttc cacgagcggc ggcgccaccg atgtggaagg tgcgcatcgt
     1801 gagctgcgtg ccaggctctc cgatcgactg cgcggcgatg ataccgaccg actcaccgat
     1861 gttgacgcga tagcctcgcg cgaggtcacg cccataacac ttggcacaaa cgcctcgacg
     1921 agcttcgcag gtcaggccgg agcgaatgac gacttcctcg ataccggcgt cttcgatcgt
     1981 tttgaccaag gtttcttcga gttcggtatt ggcttccacg agcacttcgc cggtgagcgg
     2041 atcgatgacg tcttcgagaa cgacgcggcc gaggatacga tcggccaacg gttgaatcac
     2101 ttctccggct tcttcgagtt tggtgacgcg aataccatcg agggttccac aatcgtattc
     2161 ctggatggtg caatcctgac cgacatcgac caagcgccgc gtgaggtagc cggagttggc
     2221 ggtcttcaaa gcggtatccg ccaagccttt tcgtgctccg tgggtggaga tgaagtattg
     2281 caacaccgtg aggccttcgc ggaagttggc ggtgatcggc gtttcgatga tttcaccgga
     2341 gggtttggcc atcagtccgc gcatggccgc gagctgtcgg atctgctgcg tagaaccacg
     2401 tgcgccggag tcggccatga tataaatcgg gttgaagctc ggctcatcgg actgctcgcc
     2461 ggtttccgga tccaccacgg cgtcggagct tatccccttc atcatttcgc gggtgacctc
     2521 atcggcaacg gacgcccaga tatcgacgat cttgttgtag cgctcgccat cggtgatgag
     2581 tccgtcttgg tattgctcga cgacttcttc cacgcgtttt tgggcctcgg tgaggagtac
     2641 ggctttcttt tcgggaatga ccatatcgtc catacagatc gagacacccg cgtacgtcga
     2701 gtaattgaaa ccgagggatc ggagccggtc tgccagcagc acggtttcct tgtttcgatg
     2761 ctcgcggtag cacttgtcga tcagtgcgga gagggccttt ttgtcgagtg ttttgttgac
     2821 gaaatcgaag gggacgcgct tcggaaggat ttccgagatg agcaccctac cgacggtggt
     2881 ttgcacgaga tggcgttttg gacggccgag agcgtcaaga cgaggtgttc catcgtcttc
     2941 gagatcgggt acgcgcacac tgatccgcgc gtgaagatcg acttccccgt ggtcgtaggc
     3001 catacgcact tcttcgacgg aagcgaacgt gcctcgcagt ggttttccgg tgagtttccc
     3061 ttcaccatcg agcagttgga cttcccgata gcagccccgt gcgaattttc gtgcgcgcgt
     3121 ggcgtaatac aagccaagca cgatgtcttg ggtggggttg atgatcgggc ggccgctggc
     3181 ggggctcaag atattgttgg tgctcatcat gagcacgcgc gcttccattt gcgcctcggt
     3241 gctcaacgga acgtggaccg ccatctgatc gccgtcgaaa tccgcgttga aagcggtaca
     3301 aaccaacggg tgcagttgga tggcttttcc ctcgatgagg gtgggttcga acgcttgaat
     3361 accgagacgg tgcagtgtgg gggcgcggtt gagcatgacg ggatgttcgg tgatcacctc
     3421 ttcgaggatg tcccagacct ccggacgctc tttttccacc atctttttgg cacttttgat
     3481 ggtgttgacg taaccgcgtt cttcgagctt gttgtagatg aacggtttga acagttcgag
     3541 ggccatcttt ttgggaaggc cacattggtg aagacgcaac gtgggaccga ccacaatcac
     3601 ggagcgaccg gaatagtcca cgcgtttgcc gagcaagttt tgacggaagc gcccttgctt
     3661 gcctttgagc atgtccgaca aactcttcaa aggccgtttg ttcggacccg tgatcgtctt
     3721 tcctcgccgt ccgttgtcga atagcgcatc gaccgcttct tgcagcattc gccgttcgtt
     3781 gcggatgatg atctccggtg cgttgagttc gagcaagcgc ttgagacggt tgtttcgatt
     3841 gatgacgcgt cgatacaagt cgttcaagtc gctggtggcg aatcttccac catcgagggg
     3901 gacgagaggt cgcagatccg gaggcaacac ggggatgacg gtgagcatca tccagtcggg
     3961 tcggtttcca ctgtcgcgga aggcttcgac gactttgagt cgtttggcga ttttcttacg
     4021 ttttgcctcg ctggtggact cacgcatttc ggcgcgcagg tcttcggcga gttggtggac
     4081 atcgactcga cgaagcattt cgaggacggc ttccccgccc atggcggcgg taaatgcctc
     4141 atccccgtag tcgtcgagtt tctgtaagta cttgtcttca ttgagaagtt gtccgacttc
     4201 cagatccgtt tccttggggt cgatgacgac ataggcttcg cagtacagga ctttttcgag
     4261 gtccttgagg gtgatatcga ggatattacc gatgcgcgaa ggcaagcttt tcaggaacca
     4321 gatatgggcc acgggggtag cgaggttgat atgtccaagg cgttctcttc gcaccttgga
     4381 ttgaatgacc tccacaccgc atttttcgca cacgattcca cggtgcttca tgcgtttgta
     4441 cttgccgcaa ttgcactcgt agtctttgac cggtccgaag attttggcgc agaacaaacc
     4501 atcgcgttcg ggtttgaacg tccggtagtt gatggtctcg ggttttttga cctcgccgtg
     4561 ggaccattcg cgaatttttt cagggctcgc gagcgagatg cgaatggcgc tgaacgagag
     4621 tgggtccttg ggcttttcga agaagctgaa gatgtcgcgc acgatagcct ccgttactcc
     4681 gcagccgttc gctcaccagc ggagcgttcc ggtcctgtct cgatcaactc cacgttcagg
     4741 cacaacgatt ggagttcttt gatgagaacg ttgaaactct caggaagacc cgcttcgagt
     4801 tggaattcac ccttgacgat cgactcgtac atccgcgtcc gacccatcac gtcgtcgctc
     4861 ttgaccgtca agaattcctg caacgcataa gcggcaccgt aggcctccat ggcccacact
     4921 tccatttcac ccaagcgctg gccgccaaac tgcgcttttc cgccaagcgg ctgctgggtg
     4981 accaacgaat acggtccaat cgaccgcgca tggatcttgt cgtcgacgag atgatggagc
     5041 ttcaacatat acatcacgcc caccgtcaca tcctcgtcga acgcctctcc ggtccgaccg
     5101 tcgaacaaga tcgcttgacc acttcgagga agctccgcaa ggctcagtgc ttccttgatg
     5161 tcatcctcgg tggcaccatc gaatacgggt gttgcgaaaa acacgcctcg acgaagtttg
     5221 cgagcaagac gaatcgcgtc gtcgtcattc atcctatcga tgatcgcaag cagatcgtcg
     5281 ttgccgttgt acacacgctt gagccgttct cgaatggctt ctcccgagaa cttttcgtgg
     5341 accattcgtt gcagttgttc tccgagctcc cgcgctcccc atccaaggtg cgtttctagg
     5401 atttggccga cgttcatacg gcttggcaca cccaagggat tgagaacgag gtcgacgggc
     5461 tggccgtctt gcatataagg catatcctct tcgggtagga tacggctcac gacacctttg
     5521 ttgccgtgcc gcccggccat tttgtcgccg acttgcagct tgcgtttgat cgcgatgtac
     5581 actttcacca acttgatcac gcctggtggc aattcgtcac cgcggctcaa tcgctcgatg
     5641 cgctcacgga aatgttcttc tcgaatgctg atgagttcat cgagttcacg cagcttttga
     5701 cgaaccagat cggcggaagg cacttcgatc aatccccagt attttcgcgg gattgcctgc
     5761 aaacgttcga tcgtgatctc gtctcccttg gcgagaagca ccacgccctt ttcgtcgacg
     5821 agcttgccat cggtcacact tccaatgagc aagccacgca cctgacgaaa cgccgaatcg
     5881 cgaagaatct gcacttcttc atcgcgtgtt ttttccaaac gagcttgctc ttggctctcg
     5941 atctcgcgtg cgcgatcgtc tttctccgtt ccccttcgtg agaacacgcg agcgttgatg
     6001 acgatgccgc tgacgcccgg gggaacacga agagagctgt cacgcacatc gcctgctttt
     6061 tcgccgaaga tggcacgaag caatttctct tcaggagaca attgcgtttc gcctttgggc
     6121 gtgatttttc cgacgagaat atcgccaggt ttgacttcgg cgccaatgcg cacgatgccc
     6181 gactcatcca gatccttgag ggcttcttca ccaacgttcg ggatgtctcg cgtgatgtct
     6241 tctttgccga gtttggtatc gcgcgcgaca cattcgaact cttcgatatg gaccgacgtg
     6301 aagatgtctt ctttgacgat tcgttcggag atgagaatgg agtcttcgaa gttgaagcct
     6361 tgccaaggca tgaaggcaac gagaacgtta cgtccgaggg ccaactcgcc acagtcgacc
     6421 gcggggccat cggccagcac ttcgcccttt ttgacccgat cgcccgtgcg aaccgcgggt
     6481 ttttggttgt agcacgtgga ttggttggat cgtcgatact tcatcaaatg atagatatcg
     6541 gggtagtcgg cgccttcttc ttcggcgcgc acgacgatgc gagaggcatc gacgctttcg
     6601 acgaccccat cgcgggtggc tacggcacag acgccggagt ccagcgcgac acgtagctcc
     6661 atgcccgttc ccacgatggg cgcctcggtg gcgatgagag gcaccgcctg gcgttgcatg
     6721 ttggcaccca tgagcgcgcg gttggcgtcg tcatgctcga ggaaggggac gagggccgcg
     6781 gcgacggaca ccatttggtt gggggccacg tccatgatct ggatattttc gggcggaacc
     6841 atacgaaaat cgccgttgaa acgggccgat accagatggt ctttgaactt attgtccggg
     6901 gtcaggttgg cggtggcttg cgcgatgtac tttccttctt cttccagcgc gctgagccag
     6961 ttgatgtctt ccgtcacacc ttcgctgctc acggcccgat aaggcgtttc cacaaagcca
     7021 tacgcattga cgcgcgcgaa ggtagaaagc gatgcaataa ggccgatgtt tggaccttcc
     7081 ggcgtttcaa tcggacaaat acgcccgtag tgggtagcgt gaacgtcgcg gacttcgaag
     7141 ccagcacgct cgcgagtcaa cccgccaggg ccgagagccg acaagcgtcg tttatgagtc
     7201 acttcggaaa gcggattggt ttggtccatg aattgggata gctgggagct accgaagtat
     7261 tctttgacga cagcgctgac gggtttggca ttgatcaagt cgtgcggcat gagcgtatcg
     7321 atttcaggcg acatgctcat gcgttccttg atggcgcgtt ccatccgaac caatccgatg
     7381 cggtattggt tttccatcaa ctcaccgacc gctcgcacgc gccggttacc gaggtggtcg
     7441 atatcatcga cacttccgcg accgttcttg agttcgatga ggtgtcgaac ggtttcgagg
     7501 atgtcctgtt gggtaagcac gcattgatcg agggtgggcc gctgctcctc gggaaggttg
     7561 ttgtaaaact tgtagttgag tttcaatcga cccacgcgcg acaaatcgta gcgttcgggg
     7621 ttgaaaaaca agttgttgaa gagcgttttg gcggtatcga gggtaggagg atcaccgggt
     7681 cgcaagcgac gatagatctc catgatggcg tcttcggtgg ttttgacctt atcggcgatg
     7741 agcgtatcgc gcagatacga tcccacgttg agaccgtcga tgaacaagat tttgaattgg
     7801 tggacgccaa cggaagacag gtacttgtag aagtgggcga tggcgttgcg aacggtctct
     7861 tcggtggcag gttttttacc gctgggtttt tcgcagagat cgccaagcgt gacggttttt
     7921 tgagggtccg tcggacttgg gatctgcgcg tttttgatgg agtcgttgca ttcgagaatg
     7981 acttcgccgg tttccaaatt gatgacgtcg tgggcggcca ctttttccag caactcggaa
     8041 tactcgagag ggatgcgctc gattttcgcg tccttgagta gcttgatggc ctttttgttg
     8101 aacttggtgt tctttttgac gacgaccgtg ccatcgacga cgatgtcgcg cgtggagcgt
     8161 tggccaggca acaagtcata ttcgatgctc ttgctgaatt tcccaccctt ttcgaggaaa
     8221 atatcctctg tggagtagaa gtagttgagc agctcctggg tggagtagcc caatgcgcga
     8281 agcaagaccg tggcatgcat tttccgtcgg cgatcgatgc ggacgtagat gatgtctttg
     8341 gggtcgaact cgaagtcgag ccaggaaccg cgatacggga tgactcttgc ggaataaagg
     8401 agttttccgg aagagtgtgt tttgcccttg tcgtggtcga agaacacgcc tggacttcgg
     8461 tgcaattggc tgacgacgac ccgttcggta ccgttgatga tgaaggtacc ggtgtcggtc
     8521 atgagcggga tttcgccgaa gtagacttct tgttctttga tgtcgcgcac gtcgcgttcg
     8581 ccgttgtcgc gcggctcgta gatcatgagc tgcgtggtca ccttgatggg cgccgcgtag
     8641 gtcatgccgc gctggcgaca ttcgtcgacg tcatatttgg gtttttcaag gttgtaggaa
     8701 acgaaaacca actcgctggt ttggttgaag tccctgatgg gaaagacact tcgaaagaca
     8761 gcctgcagcc ctacttcggc ccgttccgaa ggaggcacgg tagcctgcag gaacttgtcg
     8821 tacgaggtct tttgaatgtc gatgagattg ggaacctgga tgatgcgctt gacgcgtcca
     8881 aggttctttc tgatcctgaa gttcgtctgc acgaccgacg ccatcgatgt agcctccaga
     8941 cgcgtcgaac acccgccggt ggttggctca caaacgcaaa ggggccggcg tcaggtcagg
     9001 aaatcctggc ctgacgtcgt cccgaaaggg cccctgggac ctgaaacgga attggacact
     9061 tacttgagct cgaccttggc tcctgcctcc tcgagcttct ttttcatttc ttccgcctcg
     9121 gcctttggaa tggcttcctt gacgaccttg ggagcgcctt ctacgagttc tttggcttcc
     9181 ttgaggccga gtccggtaat ttcgcgaacc gccttgatca cgttgatgcg gttgggtccg
     9241 caatccttga gttcgacgtc gaactcgagt ttttcttcag cgggagctgc ttcggcagcc
     9301 gcgggtccag cgacaccggc gacggccacg ggggctgcgg aaacgcccca cttttcctcg
     9361 agggtcttga ccagattggc catgtccatg acggacatct ggctcaggtg gtcgatgagc
     9421 tgctcagtgg taatatcggc catgatatca actccttaga aacgataagc gaaagccaag
     9481 gggcgttcgc gatgcattca ttgaatcctc taccagccca aagggctggc tcatccaccc
     9541 tcgagctgac gcttgcgggc gtcgagcaag taggcgaagt tctgacgggg tgctccaagc
     9601 tggcggacca gactctgcat gggagccatg aaggtggcaa gcagcatgga acggagttca
     9661 tccttgccag gcatggtggc aagaacgttc tcgacgctgg caggggatag aacgtcggat
     9721 tcgacgactc cgcctttgat cctgagcttc tggttgtctt tggcaaaagc cttgatgacc
     9781 tttgcggcga taccgggctc ttcgtaggac caggcaatgc cggtcatgcc tttgagcaaa
     9841 ggctcgacgg cttgcgcata ggggcggtcc ttgacagcgt ggtgaacgag gctatttttt
     9901 acgactttgt agtcgacgcc gactttgcga aactcctcac ggagcttggt gacatcctcg
     9961 acggtcatgc cagcgaagtc gacgaagacc gcggagacca tgcgatcgaa gcgttcgcgg
    10021 atctcgttga tgctggcggt tcgttcggcg cggttcatgg cttacttcac ctcttctgct
    10081 gcatccgcgg tatcgactcg gatgccgggc cccatcgtgg aagacagcgc gatgcccttg
    10141 aggtaggtac ccttcgctgt cgaaggtttg gcgcgtacga gcgcttggat aagggccttt
    10201 gcgttttcga cgagtgcctc gttggtgaaa gacagcttgc cgattcggca gtggacgatc
    10261 ccggcttttt ccacgcgata ttgaaccttc cccgctttgg cttcgcgaac ggctttgtcc
    10321 acatcgaagg tgaccgttcc gaccttgggg ttgggcatca agccacgaga gccgagaata
    10381 cgtcccaact tacctaccga gctcatcatg tcgggtgttg cgatcacccg gtcgaaatcg
    10441 agaaagcctt cctggacccg ctggatgagc tcatccgagc cagcgaaatc cgcgcccgca
    10501 tcgcgagcct cgcgttcttt atcccctttg gcgaagacca gcacgcgtac cttttgccca
    10561 gtcccatggg gcaatacaat cgccccgcga accatctgat cggcatactt ggggttgacc
    10621 ccaagacgta ccgcaagctc gacagtttca tcgaacttgg catacgccgc gctcttcacc
    10681 aaggagacgg cttcctccaa ggtgtgtcgt ttcgtacggt cgactctttc gagcgccgca
    10741 cgatgtttgt tggcgacttt tctcatcgca cctccttttt cggcaatcgc ctcccacggt
    10801 gcgccatgtc ccgtggtgcg gggttagacg ctccttgcgt cggtaccaag ccaacgacga
    10861 tcagtcttgc accttcacgc ccatcgatct tgccgttccg gcgattgagt tcatcgcgga
    10921 ttcgacgttg gtggtgttca gatctttgag tttcaactca gcgatttcac gaacctgctg
    10981 ggtagtgatc gttccaacct tgattttatt gggttccttc gatcccgaac ccggtttctt
    11041 ttgcagggaa agccccgcgg cttttttgat caacaccgat gctggcggcg ttttcatgat
    11101 gaaggagaac gaacgatccg cgtacaccgt gatgagcacg gggatgatca tgtccccttg
    11161 cgattgggtg cgggcgttga agtccttgca gaacgccatg atgttgacgc catgctggcc
    11221 gagagccgga cccacgggcg gtgatggatt cgcctttccc gcgggaagtt gcaacttgat
    11281 aaggccgacg atcttcttca ttgtcttgca tgctcccagc gcccacgccg cgcttcgaag
    11341 cccggggggc tggtattaac gcttctctac ctggctgaat tcgagttcga ccggcgttgc
    11401 ccggccaaaa atcgatactt tcacacgaag cttctgcttt tccggcttca cctcttctac
    11461 cgtgccagag aaattcgcaa acgctccgtc gatcacacga acctcatcgc cctcttcaaa
    11521 ggttacctgc ggcttgggct tcatcgcccc ctccaccatc ccctgccgca aaccatcgat
    11581 ctcaggcgct ttgaccggag tcggattctg gttccctatg aaccccgtca cccgattcgt
    11641 ctccttgacc aaatgccaag tcttttcgtt catctccatc tcgatgaaca catatcccgg
    11701 caaactcgtt ttctgacgaa cccgcgtctt acctccggct cgatgatctt gcaccgtctc
    11761 ggtgggaatc aaaatctcac cgaaggaatc cgtcatctca tactgcttga tccgctcctg
    11821 aagcccagac ttcaccttgg cctcgtagcc cgaataagtc tggatgacgt accacttctt
    11881 tgccatggga ttctccgggg tcaggcgcgg taaacgaggt cggtcacgaa cccccagaac
    11941 cgatccaaga gcataaaata aaccgttgca ataatgctcg ccaccactac cactactgtt
    12001 cccgtggtga cctctttttt gttcggccag gtcaccttgg ctaactccgc cgccgcctcg
    12061 tccacccatg cacgggtgtc ctcacggcga cgaacataaa taaaagcagc aatcgcggcc
    12121 agacctccca ccaccatgcc ccacgtgtct cgtgcatcct cgcccacgta cgtgatccat
    12181 cccaccttgt ccaccacact ctgtgtcgat gccaacttgg tccatacgct cgatactgag
    12241 cgaccaaaca cataagcgag tgcaattccc accgcaaaaa aaccggacat cacgtaacga
    12301 gtcgcaccaa gctgcgtggg actcagccct tgggccacat cctcccccac acgaaccacg
    12361 agcttctctt ccccctcatc gacgttcgca tccacaaggg cgtcgacttg ctcgcggtca
    12421 gactccgacg actccgacgt cgtctcgtca tcccgttcgc ttttgttgtc ggcatctacc
    12481 ctggccatgg aatgctcctg gtacctgact catcactacc cgaaagcttt gaagaaaagc
    12541 ttgggtccac tcggatcgaa gagggcaggg gcggcgggac tcgaacccga aacctgcgga
    12601 tttggaatcc gctgctctac cagttgagct acaccccttc gtgattgcga ggagaacttc
    12661 cccgatcgtc acggtgtatt tatttcgtct ctcgatgtac cgtatgccgt ttgcacgttg
    12721 gacagtactt tttcatctcc aaggagggtt tcgtttctcg cactcttgtc gttcgataat
    12781 tccgcgccgc gcattccaca caagccaaaa ctaccttgag gcgatctccc aacggttatc
    12841 tcactccacg atctcggtca cgatgcccgc acccaccgtg cgaccgcctt ctcgaatcgc
    12901 aaatcgcatc tgctcttcga gcgccaccgg cgtgatgagt tcaatgctca tcg
//
LOCUS       DAOSOR010000095        12951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4831_length_12951_cov_4.769851, whole genome shotgun sequence.
ACCESSION   DAOSOR010000095 DAOSOR010000000
VERSION     DAOSOR010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 12951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12951
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4831_length_12951_cov_4.769851"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PK710_07260"
     CDS             complement(<1..292)
                     /locus_tag="PK710_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09549.1"
                     /translation="MKRKRASGVRLSVFLPIGLWTVFSLGCAYPDYVYTDDNKAETGA
                     GVGATGGTSGAEGSTGGMGGSGGGSEGGTGGTSGKGGAGGSGGAGATGGAGGD"
     gene            590..1243
                     /locus_tag="PK710_07265"
     CDS             590..1243
                     /locus_tag="PK710_07265"
                     /inference="COORDINATES: protein motif:HMM:NF037007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT09550.1"
                     /translation="MAPFHNSPKNKRDAEATQTRILRAAEELFVRHGFDGTRIDAIAE
                     AARSNKRMIYVYFGNKEQLYLEVLKRNFEKVIPLARLVDPAQTPTEQATTVISHYFRY
                     LSDNPAFLRLLGWESLREGRLAGKALVDVAAAEFEALHGILQRGIEQGEFRPDLDIPK
                     TVMSITALCFSFFNRRFLWATLWGQDLTRPETLNDVLAHTLDLVFRGIRSPCSSPKA"
     gene            1270..2229
                     /locus_tag="PK710_07270"
     CDS             1270..2229
                     /locus_tag="PK710_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOT09551.1"
                     /translation="MRSIGYVNLVFAALLLSACGHDAPSPKHVTGLVDATEIDVASKI
                     PGRVVELEVREGDRVEKGKKLAKIESEEIGAKMQQVKAAMEAAEAKLRMARSGARQEE
                     VEAARRQVESAKHQVQIAQKTYERVEKLHEANAIADAKLDEAKFQYDVARDQLAVAEA
                     KLTVLRKGARPEEIDALAALVDQAQATLTEVTVYEKEMVQTAPIAGEVSKIVVHRGEL
                     AATGYPIVTLVDLSDLWVTFTLREDALRDVRKGTTLRVFVPALDREAQLEVFHIAAMG
                     DFATWRATSERDAFDLKSFEVRARPLAPIEGLRPGMTIRWYPE"
     gene            2233..3366
                     /locus_tag="PK710_07275"
     CDS             2233..3366
                     /locus_tag="PK710_07275"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT09552.1"
                     /translation="MRDFSNGFFAQAKTLFTTPKHLIMLALLIFMSVVTAEVYRGMVL
                     RDVPVAILDLDQSSLSRTLVRMVDATREVRVVHPPISSVEEAQQWLLRGEGAAVMLIP
                     SKFSSDLKHGREPRILIAIDGSNIVLAKNVNKALATAVATVSAGAQLTFVRKLGLSES
                     QAMAAVQPIAIDINDSFNPANNYAVYIVPGSVFFLLHVYAMLLFASVFLPESASPSRS
                     HRVGRLGAIAVVTLLLGIAFFLILMPIAQVSLQSNATVAICVLGAFLVVEALFAAALA
                     KALPGPLFAFQVTVILTMLALMLSGITWPIDRFPEPLQQFSSFLPFTPFARALRIFVH
                     EPVGLDQLRLPLRQLAQQAGVFAAITLVATLSRAFIARFREVA"
     gene            3366..4586
                     /locus_tag="PK710_07280"
     CDS             3366..4586
                     /locus_tag="PK710_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT09553.1"
                     /translation="MKTWLRTWLAGSLEECKHIGRNPSILLILVAIPVVYPTLLSWLY
                     AQNAATERPAVIVDHDASVLSRKLLLSLDATQGISVVGRVDDLEQGFEMVRQRQAEML
                     LFIPEDFTSQIKSGRQASVKVWIDSANILTYGSALAATRETIGTMNEQLGRDYLHQQG
                     LTTRLADRRIMPIQTDTRLLFHPTGAYGEFLITGAFLIVFQQLILISLSFSMGLRKEL
                     TPISPRLRFPFARLAGRVAAHSLLYLLAIVFIVLVVTPSFGWRIQSSMAVFVLYVVFG
                     LTMFPLAMIAASLVKSRYTGFQVLMFLSTPVFMMSGFTWPLDQMPRTIQLLASAFPAT
                     PALLAMRTLAVKSGSFSAIRTELAWMAAQFIAYTLVAIALLHGQERRKLLNSNEINRE
                     LAGGDEPGTASQTA"
     gene            complement(4602..6410)
                     /locus_tag="PK710_07285"
     CDS             complement(4602..6410)
                     /locus_tag="PK710_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09554.1"
                     /translation="MKKFACLVLSATALIFGCNAESTSDSPPQQEQDSGTDTQESTIT
                     VCSTVEILPSTCVALSDHFTQSTTLAKGCYRANTSPTLDEGVSLTLQPGVTILFSEGT
                     KMEIAGNRTLIAEGTTQNPICLSSEKAARGSWIGLDFENNDNPSKLAYVTIEYAGNTT
                     TDPTRGAVQALADSGGVTLEMSNTTIRDSEGYGLRIGASTHLPAFSGNTFTKNKLGPV
                     LADSEVVGLLDAASRYTGNDKDEITVPSESLSKNATWKSLGVPYHLIGTLGLSVETHW
                     TLEAPNTVIMPAAVGISVSGDDAALTAVGTKDNPIVFTAESKTKGYWDGIVFDNSMNT
                     NNKFDYVTVEYAGDTSSVADQAAVRASADEHGVTLSITNSTLRESNGYGLYLAGTAVL
                     PNFGNNTFTQNTLGPVNVGFEAVAQLETSSTYTGNDVDQIRVREGNVAESTSWADLGA
                     PYHLESSVHVLKVWTLNPGVTLLMAKDTWISVEGDDAGLHAVGTDTQPITISGVEKTA
                     GYWHAILFDGSINPDNTLEYVTVEYGGSPTGGGEEGMITAASDSHGVSLQVKHCTIKD
                     SGQWGIWLGKFAEVNSDIETINTFSNDAKGNVYRQP"
     gene            complement(6680..10411)
                     /gene="mfd"
                     /locus_tag="PK710_07290"
     CDS             complement(6680..10411)
                     /gene="mfd"
                     /locus_tag="PK710_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOT09555.1"
                     /translation="MTAASESSAFAQLGDRLRLSLEHPSQDPESLLDPLPDYDPPPDP
                     ILIDTATIARHLHQTSRLHVTGCHGSATAWAACAIARTRNHPVLCITATPEQAQQLVL
                     DLQFLWGQDQRESDDAVPGQVLSLTATEASPYAEVNPDRRGAHHRLATLSHLASDLPF
                     RFLVTHGTALLRKIVPRSVVEKYTHLIEIEQEIDRAALIDKLSESGYLRSPLVEDPGT
                     FAIRGSLVDVWAAGSKTPIRIEFVGDVVVSLKAFDPDTQRTLGPVVKCWLPPAREAIR
                     TADSMDRARRVVRELCDTVDLPSSRTHALVEDIVSGRLFLGLEGMLPAYFELTSIGEY
                     LTRDYTVVMQDPDAIARAVHDEYEQAQRDEMVQAMLRKQASGPPSMSLPRFAMQDYFT
                     SEKEVSELISRWPMLVVHPTATVGQEQEPTALAEYSTTPPDTPTLGTQDLSELGRAIQ
                     SARMRGGKASALTPLIRRIQRWRELGLRVLLTARADSQAERLAALLRYRGIDCRAKLG
                     PFDPTILQTPGEPVLVVRGALSQGVVAPTEGWVLVTEEEVFGRRAKRRAQRARSSSLR
                     QPFIQDLRALSPGDLVIHVEHGVGKYLGLLHKKIDATTVDLIAIEYAGGDKLYLPVYR
                     LNQIQKHTAADAKQKLDRLGGLTFARTKSRVRANVRKMADELLQLYAQRQALAVLPLS
                     DVDDEYRAFEATFPFEETRDQAIAIDDVMRDLTSDRPMDRLVCGDVGFGKTEVAIRAA
                     FRAASQSRQVAVLCPTTVLAQQHLMSFRSRLESYGIDVRGLSRFQSKKEQTEVLRGLR
                     AGFVDVVIGTHRLLSKDVHFKSLGLLVVDEEQRFGVAHKERIKQLKQSVDVLSLSATP
                     IPRTLQMAVSGLRDISLIGTPPVDRRAIRTIVTRQDDRVLREAIERELSRNGQVFFVY
                     NRVEGLYERAAKLQELLPEARIAVAHGQMPEATLERTMLDFVEGRYDVLASTSIIESG
                     IDIPRANTIIIDRADMFGLAQLYQLRGRVGRSKERAYCYLVVPPVDAMTDEARARVEA
                     IEQHSELGSGFHIASLDLELRGSGDLLGAEQSGNVASVGFDLFRQMLEDAVHELRGEP
                     VIHDIEPELSFDVEALLPEDYIADIGVRLSFYQRLASASDEQDVEELSIEMQDRFGAP
                     PEAALHLVQLMRQKTELRRIRVLACEANARSVVLHLADDHLLDSEKLIRLVQQRPRAF
                     QVSPDMRLFRKATEKDAWKNGIEALETTLQEIGAVREAR"
     gene            complement(10437..11867)
                     /locus_tag="PK710_07295"
     CDS             complement(10437..11867)
                     /locus_tag="PK710_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT09556.1"
                     /translation="MTSTTSAMQQYGIPASPESASRGGTSAGLVLLYSQSYKQLQPAY
                     PLTTSEVFIGRDPLCAIVVPERAVSRHHAMLKYNGESWLLRDLGGANGTMVDDHFIDE
                     IELEHLHEIRVGDTVMKFVLAGAENYMSYGIDGAMVGSVQRRSTRLTELVGGWAIDRI
                     GAQLERIAPLELLIVIQGESGTGKEVVAREIHRLSGRAGPLRAVNCAAIPPNLIESEL
                     FGYKRGAFSGAERDKLGLIPAANHGTLLLDEIGDMPLEAQAKLLRVIQSGEIYPVGAT
                     TAQHVNVRFICATNRNLPNLVKEGRFRGDLLARLNEFSIELPPLRERKEEIFQLSRTL
                     IARHGHADKQISFPFMLALLHYDWPYNVRELEACLRRAVAIADTPVLDPRHLPDCIRE
                     AMVPYGTRLAPSSEPAPIAVHEPSPAAPASCRPRASAPSEQRMRELLAAHRGNVAAVG
                     RELGKERMQIHRWMQRYGIVVDEYRR"
     gene            11906..12730
                     /locus_tag="PK710_07300"
     CDS             11906..12730
                     /locus_tag="PK710_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOT09557.1"
                     /translation="MRTNPEEAIEVAAVQLSAQEDVSENLRILRSMVLQAARRGARLI
                     VLPENLAFMGPERKKRQLAAPLSPTNPVFDALGAVAKEAQRWVIAGGIPEKSDDPLRP
                     YNTCAVFAPDGTLAATYRKIHLFDVDLPDGTRLMESEACLAGSAPQCVDIDGIRVGLS
                     ICYDLRFPELYRRLVSMGAQLVVVPAAFTLTTGKDHWHVLLRARAIENQVYVVAAAQF
                     GAHGARTTYGKALIVDPWGTIVAQCSDHVGFCQHPIDLHYLRQVRRSLPCLQHRRL"
     gene            complement(12744..>12951)
                     /locus_tag="PK710_07305"
     CDS             complement(12744..>12951)
                     /locus_tag="PK710_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09558.1"
                     /translation="YTSETSIDFGDRIGHDPHPQRRFAYDGGRKRLWMALDGRLVAFD
                     RSVTGFEQVDLTLGYRAKAVAIVW"
BASE COUNT         2599 a         3784 c         3686 g         2882 t
ORIGIN      
        1 catctccgcc tgccccgccg gttgctcctg ccccgccgct gccgcccgcg cctccctttc
       61 cgcttgtgcc tccggttccg ccttcagaac cgcctccgct tccccccatg ccccccgttg
      121 acccctccgc gcctgacgtg ccgccggtgg cgccaacccc ggcacctgtt tctgccttgt
      181 tatcgtctgt gtaaacgtag tctgggtacg cgcaccccag ggaaaacaca gtccacaggc
      241 cgatgggcaa aaaaactgat aagcgcaccc ccgatgcccg tttccgtttc accgtgaagc
      301 ctccgccttc cagtatacta cgacactacg gttcgcgtag ggaaaaacag cgatacgtag
      361 aaaatcgatc cttcggtatc ttgatttgac ttgcattgag gccgacaatt ccgctgctcg
      421 aggcccaaca gccgatcata tcccatcggg aacgaacgag gcggggagcg acgggagggc
      481 atgggttccg agggtggctc catcggtaag cctggatgtc attttgttcg gggttgctct
      541 tgacacgagt cggtcttgga tccaataagt aaccatccgg ttagttatca tggccccttt
      601 tcataattcc ccgaaaaata agcgtgatgc ggaagctacc cagacccgca tccttcgcgc
      661 cgcggaagaa ttgttcgtgc gtcatggctt cgacggcaca cgcatcgatg ccatcgctga
      721 ggcagctcgc agcaacaaac ggatgatata tgtatatttc ggaaataaag agcaattgta
      781 tttggaagta ttaaaaagaa atttcgagaa agtgattccc ctggctcgtc tcgtcgaccc
      841 cgcccagacc ccaaccgagc aagccaccac ggtcatttct cattattttc gttatctatc
      901 ggataatccg gcctttttac ggcttctggg gtgggagagc ttgcgcgaag gtcgcctcgc
      961 gggaaaagcc ctggtcgatg tggctgccgc cgagttcgaa gcccttcatg gaattctcca
     1021 acgcggcatc gaacaaggcg aattccgccc cgatctcgat atccccaaaa cggtgatgag
     1081 catcaccgcc ctgtgtttca gctttttcaa ccgacgattt ctctgggcaa ccctctgggg
     1141 ccaggacctg acgcgtcctg aaacgctcaa tgacgtgttg gctcataccc tcgatctcgt
     1201 ttttcgggga attcggagcc cttgcagctc acccaaggcg tgaacttctt cgattccatg
     1261 aggtgcgacg tgcgttcgat aggctacgtt aatctggttt tcgcagcgtt attgctttct
     1321 gcttgcggcc atgacgctcc ctcccccaaa cacgtgaccg gcttggtcga tgccacggaa
     1381 atcgatgtcg cttcgaaaat ccctgggcgc gtggtcgaat tggaggttag ggaaggggac
     1441 cgcgtagaaa aggggaaaaa actagctaaa atagaaagtg aagaaattgg ggctaaaatg
     1501 cagcaggtca aagccgcgat ggaagcggcc gaagccaaat tacgcatggc ccgcagtggc
     1561 gcgagacaag aagaggtgga agccgctcgt cgccaggtcg aatcggcaaa acatcaggtg
     1621 caaatcgctc aaaaaaccta tgagcgggtg gaaaaactcc acgaggccaa cgcgattgcc
     1681 gacgccaaac tcgatgaagc gaaattccaa tacgatgtgg cccgcgacca gctcgccgtc
     1741 gccgaggcca aattgaccgt ccttcgcaaa ggcgcgcgcc ccgaagaaat cgatgccctg
     1801 gccgccctgg tcgaccaggc gcaagccacc ctcacggaag ttaccgttta tgaaaaggaa
     1861 atggtccaaa cggcaccgat cgccggagaa gtatccaaaa tcgtcgtgca tcgaggagag
     1921 ctggcggcaa caggctatcc catcgtaacg ttagtggatt tatcggatct atgggtgacc
     1981 tttacccttc gcgaagacgc gttgcgcgat gtccgcaaag gcacgaccct tcgcgttttc
     2041 gtacctgccc tcgaccgcga agcccaactc gaggtctttc atatcgccgc gatgggcgat
     2101 ttcgccacct ggcgcgcgac ctcggaacgc gatgccttcg atctcaaaag cttcgaagtc
     2161 cgcgctcgtc ccctcgcccc catcgaaggc ctgcgcccag gcatgaccat tcgctggtat
     2221 ccggagtgag ccatgcgaga cttttccaac ggctttttcg cgcaagccaa aaccttgttc
     2281 accacgccca agcatctgat catgcttgcc ttgttgattt tcatgtccgt ggtgacggcc
     2341 gaggtctatc gcggcatggt ccttcgcgat gtgccggtgg ccatcctgga cctggatcag
     2401 tcgtccctca gccggaccct ggtcaggatg gtggacgcaa cccgcgaggt gcgcgtcgtt
     2461 catcccccca tttcttccgt cgaagaggcc caacaatggc tcctgcgcgg cgaaggcgct
     2521 gccgtcatgc tcattccttc gaaattctcc tccgacctga aacacggaag agaaccgagg
     2581 atcctcatcg ccatcgacgg cagcaacatc gttctcgcga aaaacgtcaa caaagccctg
     2641 gctaccgcgg tagctaccgt ttcggccggt gcccagctca ccttcgtacg aaaattgggg
     2701 ctgagcgaaa gtcaggccat ggccgccgtc caacccatcg ccatcgatat caacgacagc
     2761 ttcaatcctg ccaataatta cgccgtgtat atcgtcccag gctcggtttt ttttctttta
     2821 cacgtctacg ccatgctcct ttttgcctcg gtgtttctgc cggaaagcgc atccccaagc
     2881 cgttcccacc gcgtggggcg cctgggcgcc atcgccgtcg tgaccctgct gctcggaatc
     2941 gcgtttttcc tcattctcat gcccattgcc caggtatcgc tgcaaagcaa cgccacggtg
     3001 gccatctgcg ttcttggcgc gtttttggtc gtggaagcct tattcgccgc tgcgcttgcc
     3061 aaagcgctcc ccggccccct gtttgcattc caagtcaccg tgatcctcac catgctcgcg
     3121 ctgatgctct ccggcattac ctggcccatc gatcgattcc cagagcccct gcagcaattc
     3181 tcctcctttt tgcccttcac cccctttgcc cgagccttgc gcatcttcgt gcatgaaccc
     3241 gtcgggctcg atcaactacg gctccccctg cgccaactcg cccaacaggc cggggtattc
     3301 gccgccatca ccctcgtcgc gaccctgtct cgcgccttta ttgcccgatt ccgcgaggtg
     3361 gcctgatgaa aacctggctt cgaacctggc ttgcgggaag cttggaggaa tgcaagcaca
     3421 tagggcgtaa cccatcgata ttactcattt tagtggcgat tccagtcgtg tatcccacgc
     3481 ttctatcctg gctatacgcc caaaacgccg ccaccgaacg tcccgcggtg atcgtggatc
     3541 atgacgcctc cgtgctatcg cgaaaactgc tgctttccct ggacgcaacc caagggattt
     3601 ccgtggtggg aagagtcgat gacctcgaac aaggcttcga gatggtgcgt cagcggcaag
     3661 cggaaatgct gctgttcatc cccgaagact tcacgtctca gatcaagtcc gggcgtcaag
     3721 cctcggtcaa ggtctggatc gatagcgcca atattctgac gtacggcagt gcgctggccg
     3781 ccacccgcga aaccatcggc accatgaacg agcagttggg tcgcgactac ttgcaccaac
     3841 aagggcttac gacccgactg gcggaccgtc gcatcatgcc gattcagacg gatacacgat
     3901 tgctgttcca tccgacgggc gcgtatggcg aatttttgat aacgggtgca tttcttattg
     3961 tatttcaaca gcttatattg atttcgctat cgttttcgat gggcttgcgc aaggaactga
     4021 ccccgatttc accccggctc cgtttccctt ttgctcggct ggccggccgt gtcgctgccc
     4081 actcgctgct ttacctgctt gccattgtct tcatcgtgct ggtggtcacc ccctcctttg
     4141 gttggcgcat ccagtccagc atggctgtat ttgtgttgta tgtggtgttt ggcttgacga
     4201 tgttcccgtt ggccatgatc gccgccagtc tggtcaagag ccgctacacc ggatttcagg
     4261 tattgatgtt cctttcgact ccggtgttca tgatgtcggg cttcacatgg cccctcgatc
     4321 aaatgcccag gacaatccag ctccttgcgt cggcctttcc cgctacgccg gcgttgctgg
     4381 ccatgcgcac gctagctgtt aaaagtggaa gtttttccgc gattcgcacg gagctggctt
     4441 ggatggcagc acaattcatc gcgtacaccc tcgttgccat cgctttactt catgggcaag
     4501 agaggcgtaa attattgaat tcaaacgaaa ttaatagaga gctggcaggt ggggacgagc
     4561 ctgggaccgc gtcgcaaacg gcttgagtcg tcgtctgcga ttcatggttg tcgatagaca
     4621 ttgcctttcg catcgttcga gaacgtgttg atcgtttcga tatcactgtt gacctctgcg
     4681 aatttgccaa gccaaatacc ccattggccg ctgtccttga tggtgcagtg tttgacctga
     4741 agcgacacac catggctatc gcttgccgcg gtgatcatcc cctcttcccc gccacccgtc
     4801 ggtgaaccgc cgtattcgac cgtgacgtat tccagtgtat tgtcaggatt tatggagcca
     4861 tcgaacagga tggcgtgcca atagccggcg gttttttcaa ctccggagat ggtgatgggt
     4921 tgggtgtcgg ttccgaccgc gtgcaagccc gcgtcgtctc cctccacgct gatccacgta
     4981 tctttggcca tgagcagcgt aacgccggga ttgagagtcc agaccttcag cacatggacg
     5041 ctgctttcga gatgataagg ggcgccaaga tcggcccaag aagtcgattc ggcgacattg
     5101 ccctcccgga cacggatctg atcgacatca tttcccgtat aggtggagct tgtctcgagc
     5161 tgtgccacgg cttcgaaacc cacgttgacg gggccaaggg tgttttgcgt gaaggtgttg
     5221 ttgccgaagt tgggcaacac ggccgtgccc gcgagataaa ggccgtagcc gttgctttct
     5281 cgcagcgtgg aattggtgat gctcaaggtg acgccgtgtt cgtcagcgct tgcccgcact
     5341 gctgcctggt cggcaacgct gctggtatcc cctgcgtact cgacggtgac ataatcaaat
     5401 ttgttgttcg tattcatgga gttatcgaat acgatcccgt cccaatagcc tttggttttg
     5461 gattccgcgg tgaacacgat ggggttgtct ttggtgccga ccgcggtgag cgcggcgtcg
     5521 tcgcccgaga cggaaatacc cacggccgcg ggcatgatca cggtgttggg ggcttcgagg
     5581 gtccaatggg tttcgacgct cagtcccaag gtgccgatga ggtggtaggg aacaccgagg
     5641 gatttccatg tggcattctt cgacaaggac tcgctcggga cggtgatttc gtccttgtca
     5701 ttgccggtgt atcgagaagc ggcgtcgaga agtcccacga cttcggagtc ggccaagacc
     5761 gggccgagtt tgtttttcgt gaaggtgttg ccagaaaaag cgggcaaatg ggtgctggcg
     5821 ccgatgcgca gcccataccc ttcactgtcg cgaatcgtcg tattcgacat ctccaaggtg
     5881 accccgccgc tatcggcaag tgcttggaca gcgcctctgg tcgggtcggt cgtggtattg
     5941 ccggcgtatt cgatcgtcac gtaagcgagt ttgcttgggt tatcgttgtt ttcgaagtcg
     6001 agcccgatcc atgagcctcg cgccgccttt tcgctcgaca agcagatggg gttttgcgtc
     6061 gtgccttcgg caatcagcgt ccgattgcct gcaatctcca tttttgtccc ttcggagaaa
     6121 aggatcgtga ctcctggctg aagggtgaga ctaaccccct cgtcaagcgt cgggctcgta
     6181 ttggccctgt agcagccctt ggccaaggtc gtggattgcg tgaaatgatc gctcagggct
     6241 acacaagtcg agggcaggat ttcgacagtc gagcataccg tgatcgtgct ctcttgggtg
     6301 tcggtgccac tgtcttgctc ctgttgtggg ggcgaatcgg atgtggattc ggcgttgcat
     6361 ccgaaaatga gtgcggtagc ggataagacg agacaagcaa attttttcat cgaaaagaac
     6421 ctccatcggg agagaactga agtccccaaa actgcttcct tgacctcaga tagggggatg
     6481 attccttgtg gctttcacaa ctcgttccac cggcaaagcc acgctgtcgt ttggtacgat
     6541 gagaaaaaaa tatttcttcc tagcttcgat cgataattgt ttcgatgctt ggaaatcgtg
     6601 gcgatggcgt ggtgttcacg aatgcgttcg atggagtcat ttcgtcatcg aggggcagac
     6661 aacgctcatg cgggctcggt catcgtgcct ctcggacggc gccgatttcc tgcaacgtcg
     6721 tttccaacgc ttcgataccg tttttccacg catctttttc ggtggctttg cgaaatagac
     6781 gcatgtctgg gctcacctgg aacgcccgag gccgttgttg cacgaggcgg atgagttttt
     6841 cgctatcgag gaggtgatcg tcggccaggt gaagcacgac ggagcgagca ttggcttcgc
     6901 acgcgaggac acggatgcgt cgtaattccg ttttctgccg catcaactgc acgaggtgca
     6961 aggcagcctc gggcggggca ccgaagcggt cttgcatttc gatgctcaac tcctcgacat
     7021 cctgctcatc ggacgcactg gccagccgtt ggtagaacga aagtcgcacg ccgatatcgg
     7081 cgatatagtc ttcgggaagc agggcttcga catcgaagct cagttctggt tcgatatcat
     7141 ggatgaccgg ttctccccgc agttcgtgga cggcgtcctc gagcatttgg cggaacaagt
     7201 cgaatcccac ggaggcgacg ttgccgcttt gttccgcgcc gagcaggtct ccggagccac
     7261 gtagttcgag gtcgagcgag gcgatatgaa agcccgaacc cagttccgag tgctgttcga
     7321 tggcttccac gcgcgctcgg gcctcatcgg tcatggcgtc gacggggggc acgaccagat
     7381 agcaataggc acgctctttg gatcgaccga cacgtccgcg gagctgataa agctgcgcga
     7441 gcccgaacat atccgcgcga tcgatgatga tggtgttggc ccgagggata tcgataccgc
     7501 tctcgatgat cgatgtggaa gccaaaacat cgtatcgtcc ttccacgaag tcgagcatcg
     7561 ttcgttccaa ggtggcttcc ggcatttgtc cgtgggccac ggcgatgcgc gcttcgggga
     7621 gcaattcctg cagctttgcg gcccgttcgt acagcccttc cacgcgattg tagacgaaaa
     7681 acacctgtcc atttcgggat agttcccgtt cgatggcttc ccgcagcacg cgatcgtcct
     7741 gacgtgtcac gatggtgcga atggcgcgac gatcgacggg tggtgtgccg atgagggaga
     7801 tatcgcgcaa cccgctcacg gccatctgca gcgttctcgg gatgggcgtg gcggacaacg
     7861 aaagcacgtc gaccgattgt ttgagttgct tgattctctc tttatgagcc acgccaaagc
     7921 gctgctcctc gtcgacgacg agcaatccca aggatttgaa gtggacgtct ttggacagca
     7981 atcgatgggt gccaatcacc acgtcgacga agcctgcacg aagcccgcgc agcacctcgg
     8041 tttgctcttt tttgctttga aatcgggaca gtccgcgaac atcgatgcca tagctctcga
     8101 gtcggcttcg gaacgacatc agatgctgtt gggccaggac cgtggtgggg cacaacaccg
     8161 ccacttgccg cgattgactt gccgcacgaa atgcagcgcg gatggcgact tccgtttttc
     8221 caaatcccac atctccgcag acgagccgat ccatcggacg atccgaagta agatcccgca
     8281 ttacatcgtc gatggcgatg gcctgatcgc gtgtttcttc aaaaggaaac gttgcctcga
     8341 acgcgcgata ttcatcgtcc acatccgaaa gcggcaggac ggccagggct tgccgttgcg
     8401 cgtagagctg gagaagctca tcggccattt ttcgaacgtt ggcgcgcacg cgggacttgg
     8461 tgcgcgcaaa ggttagtcca ccgaggcggt cgagcttttg ttttgcatcc gcggcggtat
     8521 gcttctggat ttgattgagc cggtagaccg gaagatagag tttgtctccg ccggcatact
     8581 cgatggcgat gagatcgacg gtggtggcgt cgattttttt gtggagcaat cccaggtatt
     8641 tgcccacgcc atgctcgaca tggatgacga ggtcaccggg gctcagagca cggaggtcct
     8701 ggataaaagg ctgacgaagc gaggaagagc gagcgcgctg tgcccgacgt ttggcgcggc
     8761 ggccgaaaac ttcttcttcc gtgacgagga cccacccttc ggtgggtgcg acgacccctt
     8821 gggatagcgc gcctcggacc acgagcaccg gttctccggg cgtttgcaat atcgtgggat
     8881 cgaaaggacc gagcttggcg cgacagtcga tgccccgata acgaagcaag gccgcaagac
     8941 gctctgcttg tgaatctgct cgcgcggtga gcagcacgcg caaccccagt tcgcgccacc
     9001 gttggattct tcggatcaaa ggagtcagcg cacttgcttt tcctccgcgc atgcgcgccg
     9061 attgaatcgc tcggcccagc tccgataaat cttgcgtgcc aagcgttggc gtatccggtg
     9121 gcgtcgtcga gtactccgcc aacgcggttg gctcctgctc ttgccccacc gtggccgtcg
     9181 gatgcacaac gagcatgggc caccgagaaa tgagttcgct tacctctttt tcgctcgtga
     9241 agtaatcctg catcgcaaag cgcggcagac tcatggacgg cggaccgctt gcctgcttgc
     9301 gcaacatcgc ctggaccatt tcatcccgtt gcgcttgctc gtactcgtcg tgaacggcgc
     9361 gcgcgatcgc gtccggatcc tgcatcacca ccgtgtagtc ccgggtcaaa tactcgccga
     9421 tcgatgtcag ttcgaaatag gcgggaagca taccttccag acccaaaaac aggcggcccg
     9481 atacgatgtc ttccaccaac gcgtgggtgc gagaagaagg cagatccacc gtatcgcaaa
     9541 gctcgcgcac cacacgacgc gcacggtcca tcgagtcggc cgtacgaatg gcctctcgtg
     9601 ccggaggcaa ccagcatttg accactggcc cgagcgtacg ttgcgtatcc ggatcgaatg
     9661 ccttcagaga tacaaccacg tctccgacga actcgatgcg tatcggagtc ttcgacccgg
     9721 cagcccagac atcgacaagg gaacctcgaa tggcgaaggt gccaggatcc tcgacgagag
     9781 gcgagcgtaa atatccggat tcggatagtt tgtcgatcag cgctgccctg tcaatttctt
     9841 gttctatttc aatgagatgt gtatattttt cgacaaccga tcgaggaacg attttgcgca
     9901 atagcgcggt gccgtgagtg accaaaaacc gaaaaggaag atcggaagcc aggtgagaga
     9961 gggtcgctag ccgatgatgg gcgccgcgac gatccgggtt gacttccgcg tacgggctcg
    10021 cctcagtggc ggtaagcgaa agaacctgtc ccgggacagc atcgtccgat tccctttggt
    10081 cttgtcccca taggaattgc aaatccaaaa cgagctgctg cgcctgttcg ggtgtcgcgg
    10141 tgatgcagag caccggatgg ttacgagtgc gggcgattgc gcaggcagcc catgccgtgg
    10201 cactgccgtg acaccccgtg acatgcaggc gagaggtttg gtgcaaatga cgggcgatgg
    10261 tggcggtatc gatgagaatt gggtcgggcg gggggtcgta gtcgggaagc gggtcgagca
    10321 ggctttccgg atcctgcgag gggtgttcga gggaaagcct caatcggtcg ccaagctgag
    10381 cgaaagcgga agactcggaa gcagcggtca tgtatttccc aggataagag cgaaaatcag
    10441 cgacgatact cgtccacgac gatgccgtag cgttgcatcc atcgatggat ttgcatgcgt
    10501 tctttgccga gttctcgtcc tacggcggcg acatttcctc ggtgggcggc cagcagttcg
    10561 cgcattcgtt gttcggaggg ggcggaagcc cgaggccggc aggaggcagg agcggctggt
    10621 gatggttcat gaaccgcgat gggcgcgggt tcggatgagg gggcaaggcg tgtgccgtac
    10681 gggaccatcg cctcacggat gcagtcgggt aagtgcctag ggtcgagcac gggcgtgtcg
    10741 gcgatcgcga cggctctgcg aagacacgcc tctaattccc gtacgttata aggccagtcg
    10801 tagtgcaaca gggccagcat gaagggaaaa gagatttgct tgtcggcatg tccatgccgg
    10861 gcgatgagcg tgcgagagag ctgaaagatt tcttcttttc gttcgcgtag gggtggcaat
    10921 tcgatgctga actcgttgag acgagccaat agatctccgc gaaaacgtcc ttctttcacg
    10981 aggttaggca ggttacggtt cgtggcgcag atgaaccgaa cgttgacgtg ttgcgcggtg
    11041 gtggcgccaa ccgggtagat ctccccggac tgaatgaccc gaagcagctt ggcttgcgct
    11101 tcgaggggca tgtcaccgat ttcgtcgagc agcagcgttc catgattggc cgcgggaatc
    11161 agacctagtt tgtctcgttc ggcgccggaa aacgcccccc gcttataacc aaatagctcg
    11221 ctttcaatca gattgggggg aatcgcggcg caattgacgg ctcgcaaggg gcccgcgcgc
    11281 cccgataagc gatgtatctc tcgagccacc acttccttac ctgttccgct ctcaccctgg
    11341 atcacaatca acaactcgag cggcgcgatt cgctcgagct gcgccccaat tcgatcgatc
    11401 gcccagcccc ccaccaattc cgtcagtcgc gtgctgcgac gttgcacgct gcccaccatg
    11461 gcaccatcga tgccatagct catgtaattc tccgcacccg ccaatacgaa cttcatgacg
    11521 gtgtccccca cacggatttc atgcagatgc tctagctcga tctcatcgat gaaatggtcg
    11581 tcgaccatcg tgccgttggc tccgcccaga tcccgcaaca gccaggattc cccgttgtat
    11641 ttgagcatgg cgtggtgacg ggatacggcg cgttcgggca cgacgatcgc gcatagaggg
    11701 tctcttccga tgaaaacctc gctggtcgta agcgggtagg ctggctgaag ttgcttataa
    11761 gattgcgaat acagcaaaac caatcccgcg gaggtgcctc cccggctcgc gctctctggg
    11821 ctcgcgggga tgccgtactg ctgcatcgca ctcgtcgtgg acgtcatggt gtcaccgctc
    11881 gaacaatcat cgtagtctac cccgtatgag gacaaaccct gaagaggcga tcgaggtagc
    11941 cgccgtacag ctttcggctc aggaggatgt ttcggaaaac ctgcgaatcc tgcgatcgat
    12001 ggttttgcag gccgcacgca ggggtgcaag gctcattgtg cttccggaaa acctggcatt
    12061 tatgggtccc gaacggaaaa aacgacaact cgccgctccc ttgagcccaa cgaatccagt
    12121 cttcgatgcg cttggcgccg tcgcgaaaga agcgcaacgt tgggtcattg ccgggggaat
    12181 ccccgaaaaa tccgacgatc ccctgcgtcc ctacaacacc tgtgcggttt tcgcccccga
    12241 cggcacgctg gccgcgacct atcggaaaat tcacttattc gacgtggatt tacccgatgg
    12301 cactcgcttg atggaaagcg aggcatgcct tgccggatcc gccccccaat gcgtggatat
    12361 cgatggaatc cgggtaggcc tttccatctg ctatgacctg cgttttcctg aattgtatcg
    12421 ccgtctggtg agcatgggag cccagctcgt cgtcgtcccc gccgccttca ccctcacgac
    12481 cggaaaagac cattggcatg ttttgctgag agcgcgtgcc atcgaaaacc aagtttatgt
    12541 cgttgccgcg gcccaattcg gagcccacgg cgcacgaact acgtacggaa aagccctcat
    12601 cgtcgacccc tggggaacca tcgtcgccca atgctccgat cacgtcggct tctgtcagca
    12661 tcccatcgac ttgcactatc tgcgtcaggt ccgtcgttcc cttccttgct tgcagcatcg
    12721 aagactatga ccagctcgtt tcatcaccat acgatggcca cggctttggc ccggtatcca
    12781 agggtaagat ccacctgctc aaaccccgtc accgaccgat cgaaagccac gagccgcccg
    12841 tcgagcgcca tccacaaccg tttccgccct ccgtcatacg caaaccgtcg ttgaggatgg
    12901 gggtcgtgcc cgatccggtc cccgaagtcg atcgatgtct cggaggtata t
//
LOCUS       DAOSOR010000096        12914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4842_length_12914_cov_3.773622, whole genome shotgun sequence.
ACCESSION   DAOSOR010000096 DAOSOR010000000
VERSION     DAOSOR010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 12914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12914
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4842_length_12914_cov_3.773622"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..1258)
                     /locus_tag="PK710_07310"
     CDS             complement(8..1258)
                     /locus_tag="PK710_07310"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HOT09559.1"
                     /translation="MMREALWFSSLLWIGCADPVSEQHALPASPSDAAYDQVSSDGQN
                     LGDGSPAGDAHDEMGPWPGMCHDGDEETCYSGSASTRDVGACRHGTHVCVGGKFPESC
                     DGEILPAEETCNGIDDNCDGTVDEGCNCQDGTTKPCYGGAPGTEGVGPCKAGTQSCVG
                     GTWGPCVGAVLPGIETCNGIDDDCNGVVDDKIGLKLYSMPFSGSAWSSQWLSEVWTGT
                     HAPPRCADVRATAFLSDINRLLVFTADGKLYWQKNGAWMPPIMASDRFPSLPTPVDAV
                     YQLPLSLFGSDLGATLTFGAVPYAYQYVYDHNDGVTKGDTFPILMQDGTSEGGPNQNQ
                     WPARWSLEYIDPSLWGQSNDAVYLYFEHDDHRVYKFNAAFVWTSWPVTESPLWSGKAY
                     APSPGTCVAGFAEQNPKKAYLVCP"
     gene            1446..2252
                     /locus_tag="PK710_07315"
     CDS             1446..2252
                     /locus_tag="PK710_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOT09560.1"
                     /translation="MVSTKNQANWTLSEEELEKLRKERGDHSTHDLTVQDLDVVFQPL
                     VDLTTGQLYAVEALVRCRWEEYRNPEIFIETAAEEGSIGKVGRLIRDVAFERCRDLPI
                     FVNVHPKELSSRWLVRPDDPINFHSEPVFIEITESAAFEYFGLCSSVMQEICSRSGAK
                     LVIDDLGAGFSNLKRVIDLEPSIVKLDRELVRGLDVNPRQRVLFQHVVRMCKELGASV
                     VAEGIETVAELEAVRKAGADYGQGYLMARPSYPIPKHHWPLAEAGRASSP"
     gene            2277..3521
                     /locus_tag="PK710_07320"
     CDS             2277..3521
                     /locus_tag="PK710_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09561.1"
                     /translation="MTKKAWTTTHRQWRRACLARLRDRRWTALAGIVFCSLQACSEPS
                     DGTSPHGAVDASPDGAADGWVESGGGDVAADIHADVSAEAGVDAADSGVDALPDNVTD
                     ALDEEAVSEASTDGNAEASAFETIELIRDYRFLQGFKTYPATPTMTPTGALIPPTATT
                     PPIWGLAEWYTTHLLPQGPPTMTALGAQWENATKRVIIRDGFLELAVNADAEYGGVYR
                     QPGQNWPHLLIQQDIFDMANPQANSPIHRFQSVTLELDVELVYAHHVKEAGYDPALHA
                     CQYLMYLTLQNRNVNHPDYGNYIWFGLTFYDDRFVTLPESIAYDVGTGKYMFQMASSA
                     FMPASLHDQPKASIHVELLPYLMASVQDAIDKGILHSSDLEDYYLGGMNMGFEVPGRS
                     VATVRVMDFHLRGVRPTNESTP"
     gene            complement(3590..4282)
                     /locus_tag="PK710_07325"
     CDS             complement(3590..4282)
                     /locus_tag="PK710_07325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOT09562.1"
                     /translation="MPKPTLPKPTASEWLERRVPLNPPDSPGEEFTSAITHGLGALAS
                     LLGSVLLIVKASSYGTVATVLGVTVFGLSMVVLYSASCLYHSARQPTFRRLARIIDHG
                     SIYLLIAGTYTPITLMIGGAWGWTLFAIVWACASIGIVLEVFKMKKSKLASLAIYVTM
                     GWIVVVAWSPLKAAVPARLLDWMLAGGVTYTLGTPFYALRSLKYHHALWHLFVLGGST
                     LFWFGIYFHACP"
     gene            complement(4403..5137)
                     /locus_tag="PK710_07330"
     CDS             complement(4403..5137)
                     /locus_tag="PK710_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOT09563.1"
                     /translation="MPRNRRCPPGDFMPFSFTRAALASLSILMMVACSSSETEDPTIV
                     AGEEHPGGGIDPGTLHQPEPGTTIPYPAGPFGRDVGSIIRNYKFSGFANPSEAEYKAT
                     KDTIVTIRLSDYYNPDNDPNKPVALLLNASARWCTVCQKEAGDSKVHYPYWKEKRVEF
                     ITAIFEDDDGEPADFSDIEYWGARFKLEYPLVLDPKLTLGAFFDKSASPFNMIIDTRT
                     MKIVFADEGLIDLGPKNATLVKLTSP"
     gene            5334..7370
                     /locus_tag="PK710_07335"
     CDS             5334..7370
                     /locus_tag="PK710_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HOT09564.1"
                     /translation="MKLAGRYRAFARWVCMLSASVLGSACGSDPEPGGTICGGVTHEG
                     QCLQRCEDSVCVQGNACAFTEDHPEGVCTLPCDNNDACPYGFACYSGIANAQGEAGQF
                     CLDLGLPEQGKPGAACTSTDDCDSLHGLYCFEARCSFPCESIFETCPDGLMCKGDPSP
                     AAGNPLGYCVPAPSDMGVDGRYGTNCPFGDSQCDEAAGFVCLGAEGTADAYCSKPDGC
                     QQDADCPSGYWCGAIRTLTEKDIDFSKQPKTCLRRGFCHPCESDLDCEYVDGAICVPD
                     TRGEKFCSTPCELDSNACLIGTSCVDGGDGRTACRPDVGYCYSKDPKGCDPCRIDPDC
                     GPNSTCVSGKWGYKPTLNWCSTPCGPNSEHGKATCPVAPNGQEMLCLDENMLSLGGPF
                     DADDPAYLYKHCYAPFTVDNTELYPGKDPPSNVCGNGRVEAGEECDDGNSYAGDGCDQ
                     CKVTAACTFTVQEPNSDGNPVVSPKPSHVKYEGEKLDPAMPYVISTAVCKTFKVEGAL
                     ETAGDVDVIAIMLPNAAEMFVDTFTSTPGTCTADLVTEARAWKNGETTDHENLLDLSV
                     PCKKLSSLPEDYASTGYACPGSPTHLGCGTCDGPGVCGVCDDDSGHGDCSRMMVSTAT
                     SFGNYPIYFDGRYKVLRVYAKDPGATVGNYILIGSRYSSQAMLGPSTPVVLSCY"
     gene            7814..10504
                     /locus_tag="PK710_07340"
     CDS             7814..10504
                     /locus_tag="PK710_07340"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOT09565.1"
                     /translation="MATISGFLWGVACGSDDDGSDASAGAGASAGASASGGQGGSGAS
                     AHHGGNSGEGAGGESTGGSGGSNVGGSGAEGGTGGTHPGGTCEPNITKSCNCTEGVGA
                     QRCAPDGTGWSACECAVYGVELAVSPNGSDTASGTPQEPFATLGRAKQEVTKIVANGL
                     PSGGVVVWIHGGDYELKEPLTLGATESGSEKAPVVWRGVPGESARILGGVKVQPGAFS
                     PIPSSSPVVARLDSDARDHVRQLDLSSQGISDFGELERRGFCRQASRSAVELFVNGVR
                     LPLARWPDATANTLPPGLETADALDIFGSPTPDVTGHYTRIGNQDGVSSFARDGLVNG
                     LQFHLYRHTWDYQGSTHTAWFLTTGSSGYPTNNDPWWYKYAHELGAMSPATGAGASGI
                     VTTRDPQGINHGFASIAEALSKTQWRYSGDRPSRWTDVSDLRFHGFWKYAWADCHVKA
                     ASIDTSTRTITLGEDTGYGIEEGQPWYVYNVPEELTEPGEWWLDRSTGLLYVWPPAGF
                     DTADVLLSTLADAIVNIKDGSWIELRDLRIEAGRAELVKLTEGTHVSLVGLTLHHAGT
                     NAVVMSGTNHRVDHCYIANPGSGGVRVSGGDRPTLLEAGNVVEHSRVEKYGQWEWTYR
                     PAVDLQGVGHIVRHNLMRDAPHSAILYSGNEHTIELNEIHGVCEYSSDAGAIYAGRDW
                     GARGNIIRHNFLHDIRTHFEGYGVHGVYLDDCLSGIRVEGNVFYKVSGMAIQHGGGRD
                     NILVNNVIARCGLGLGADKRAVDLLPKGKPNNTPGDSWNLLEKLQKVGYQDEPWASRY
                     PECAAIPNDWNAIINPGAQWLYPEGCVFSRNVGFDNSSWVNAGQGVLEVYKEYANNLE
                     DVDPLFVDEAQLNLDLQPSSPAFDIPGFEPIPFHDIGIKP"
     gene            complement(10784..11716)
                     /locus_tag="PK710_07345"
     CDS             complement(10784..11716)
                     /locus_tag="PK710_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09566.1"
                     /translation="MSEQGSSLHFRKLSIAQTKKKLATLEGSCAILASNRRWTSFVPL
                     DQASLDVARVSQLLAVTVLQVWFDEDSAVVLQIFAPDGWQAELTVPLDGTYEFGPEDE
                     RFFHGMAERKRLPSALIKELTRKMRQSQGRDSWLRSHGLEETLGLPFVIPLPVPCTEE
                     LLREIVPDAEVVRGVKKGTGKSVLPTLPQGPVARRTWSSSERAIVDLHFHYLMNLWSM
                     NDWKVYYRYKKHLPAERRGEVDNLVNMMMTGATDEEVRQALERILATIWDADDWDALI
                     RDPNLLRYEPLGQEQLQDWQRRLDETPSGGTQGS"
     gene            11777..12001
                     /locus_tag="PK710_07350"
     CDS             11777..12001
                     /locus_tag="PK710_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09567.1"
                     /translation="MAPLLKNAQGTSVYALSVPDVTPSRQMIWYHAQTRYWASDKRLP
                     TDEKWLCTAQVAVDPGSNAALGNRKCNTTG"
     gene            12049..12336
                     /locus_tag="PK710_07355"
     CDS             12049..12336
                     /locus_tag="PK710_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09568.1"
                     /translation="MYKRLGSRGYGGPLVGVDGQVVRWTKRHPELHGRRVVARGGQLR
                     LGRHVVHFASAQDGHTGWVQGLPPAALRGGNYGDGSAAGVFSMYLAEAASS"
     gene            complement(12455..12760)
                     /locus_tag="PK710_07360"
     CDS             complement(12455..12760)
                     /locus_tag="PK710_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09569.1"
                     /translation="MKMGFRHSKGGSPEPKVGYRHPKGGSPEPKVEFRNFKGGSPEPK
                     VEFRYFKGGSPEPQMGSRHPNFDSRTSENSWVDGRDGNARTCPLPKPYHPGQSLIRK"
BASE COUNT         2744 a         3536 c         3837 g         2797 t
ORIGIN      
        1 gtcggattca ggggcagacg aggtatgcct ttttcggatt ttgttcggcg aagcctgcca
       61 cacaggtccc tggcgagggg gcataggctt tcccgctcca gagcgggctt tcggtcacgg
      121 gccaactggt ccaaacgaat gcggcattga acttgtagac gcgatggtca tcgtgttcga
      181 aatagagata aacggcgtca ttcgactgcc cccaaaggga gggatcgatg tattccaacg
      241 accatcgtgc aggccattgg ttctgattcg ggccaccttc cgacgttccg tcttgcatca
      301 aaatagggaa tgtatcgcct ttcgtaaccc catcattgtg gtcgtacaca tattgatagg
      361 cataaggaac ggcgccaaac gtaagggtag ctccgagatc ggaaccaaat aaggatagag
      421 gaagttgata aacggcatcg acgggggttg gtaaggaggg aaagcggtcc gacgccatga
      481 taggcggcat ccaggcgcca tttttttgcc aataaagctt gccatcggcc gtgaagacca
      541 gcaggcgatt gatatccgac agaaaagcgg tggcccgaac atccgcgcag cgcggtggcg
      601 catgggttcc cgtccacact tcggaaagcc attgagaact ccaagcagag ccggaaaaag
      661 gcatggaata gagctttagc ccgattttgt cgtcgacgac tccattgcaa tcgtcgtcga
      721 tcccgttgca agtttcgata ccggggagca cggcgcctac gcacggaccc catgttcctc
      781 ccacacacga ttgggtgccg gctttacagg ggccgacgcc ctcggtaccg ggagcgccac
      841 cataacaagg cttggtcgtc ccatcttggc agttgcaccc ctcatcgacc gtaccatcgc
      901 aattatcatc gataccgttg cacgtttctt ccgcgggcag gatctcacca tcacaggact
      961 ccgggaattt gcctcccaca caaacatgcg tgccatgtcg gcaagcgcct acatccctcg
     1021 tcgacgccga tcccgaatag catgtttcct catcaccatc gtggcacatc cccggccacg
     1081 gacccatctc gtcatgcgca tccccggccg gactcccatc tcctaggttt tggccatccg
     1141 atgacacctg atcgtaagca gcgtccgagg gactcgcagg cagcgcatgt tgttccgata
     1201 cgggatccgc acagccgatc cacagcaagc tactgaacca taaagcttct ctcatcattg
     1261 ccgcggatga taccacaagc cacgagcaga cgctcgacca caacccaacc cccttttcgc
     1321 acactcggtc atgcctcctt gccatacgta gatcatcgtg gtgaaggcaa ggaatcaatc
     1381 gacactcccg cagaagcagc aaattacctc tggtgccaac ggcaggtgcg tggtacattg
     1441 cgagcgtggt ttccacgaaa aatcaggcga actggacgtt atccgaggaa gaattagaga
     1501 agcttcgaaa agagcgtggc gatcattcga cccatgatct gaccgttcag gatctcgatg
     1561 tcgtttttca gcctctcgtc gatttgacga ctggacaatt atacgcggta gaggctttgg
     1621 ttcgctgccg gtgggaggag tatcgaaatc ccgaaatctt cattgaaacg gcggcggaag
     1681 agggatccat cggaaaggtg ggtcgattga tccgcgatgt ggctttcgag cgatgccgag
     1741 atcttcctat tttcgtcaat gttcatccca aagagctgtc gtcacgctgg ctcgtccgcc
     1801 ctgacgatcc catcaacttc cacagtgaac ccgttttcat cgaaatcacg gaatccgccg
     1861 ccttcgaata tttcggactt tgttcgagtg tgatgcagga aatttgctct cgatcgggcg
     1921 cgaaactcgt gattgatgat cttggcgcgg gtttttcaaa cctcaaacgt gtcatcgacc
     1981 ttgaacccag tatcgttaaa ctcgatcgcg aactggttcg ggggctcgac gtcaaccccc
     2041 gtcaacgggt gctgtttcaa cacgtcgttc ggatgtgcaa ggagttggga gcctccgtgg
     2101 tcgccgaggg tatcgaaacc gtggcggaac tcgaagccgt tcgaaaagcg ggagcggact
     2161 acggccaagg atatctgatg gcccgtcctt cgtatccaat ccccaaacat cattggcccc
     2221 tcgccgaagc ggggcgtgcc tcttccccgt agctttcgag ccgacggatg agcatcgtga
     2281 ctaagaaagc ttggacgaca acacatcgac aatggcgccg agcttgcctg gctcggctgc
     2341 gagacaggcg ctggactgcc cttgcgggga tagtattctg tagcctgcaa gcgtgttcag
     2401 agccttctga tggcacgtcg ccccatggcg cagtggatgc ttccccggat ggggctgccg
     2461 atggttgggt ggaatcaggg ggcggcgatg tcgctgctga cattcatgcg gatgtatctg
     2521 ccgaggcggg ggtcgatgcg gcagattcgg gggtcgatgc cttgccggat aacgttactg
     2581 atgcgcttga cgaagaagcg gtatccgagg ccagcacgga tggcaacgcc gaggccagcg
     2641 cctttgaaac cattgaatta attagggatt atcgtttcct ccaggggttc aaaacctatc
     2701 ccgccactcc cactatgact cctacgggtg ccttgattcc acccacggcc accacgcctc
     2761 cgatatgggg tcttgcggaa tggtatacga cgcacttgct cccccagggg ccgccgacga
     2821 tgaccgcgtt gggggcgcaa tgggaaaatg ccacgaagcg agtcatcatt cgcgatggtt
     2881 ttttggaact ggcggtcaac gcggatgcag aatacggcgg ggtgtatcgt cagccggggc
     2941 agaattggcc gcacctgctc atccagcagg acatattcga tatggcgaac ccgcaggcca
     3001 actcgcccat tcaccgcttt cagagtgtga cgctcgaact cgatgtcgaa ctcgtgtacg
     3061 cccatcacgt gaaagaagcg ggatacgatc ccgcgttgca tgcctgccag tatttgatgt
     3121 acttgacctt gcaaaaccgt aatgtcaatc acccggatta tgggaattac atttggtttg
     3181 gcctgacctt ctacgacgat cgattcgtta ccctccccga aagcatcgcc tatgacgttg
     3241 gcactggaaa atatatgttc cagatggctt cttccgcctt catgccggcg tcgctgcacg
     3301 atcaaccgaa ggcatcgatt catgtcgagc ttcttcccta ccttatggcg agtgtgcagg
     3361 acgcgattga caaggggatc ctccattcat ccgacctcga agactattat ctaggtggga
     3421 tgaacatggg attcgaggtt cccgggcgtt cggtggcgac ggtacgagtg atggactttc
     3481 atcttcgtgg cgtgcgtcct acgaatgaat caacgccata gtccgcggca cgagagcctg
     3541 tcttttgccg attttattgg ggaaagaaat cgacagcgtc attgtcattt caaggacagg
     3601 catgaaagta gataccgaac caaaacaggg tgcttccccc aaggacgaat aggtgccaga
     3661 gcgcgtggtg gtacttgagg gagcgcaggg cgtagaaagg ggttccgagg gtataggtca
     3721 ctccccccgc gagcatccaa tcgagcaagc gtgcgggaac ggcggctttc agcggtgacc
     3781 aggccacgac gacaatccag cccatcgtca catagatggc gagggaagcg agtttggatt
     3841 ttttcatttt gaaaacttcg aggacgatgc cgatgcttgc gcaagcccag acgatagcga
     3901 ataacgtcca gccccatgca cctccgatca tgagggtaat gggcgtatac gtacccgcga
     3961 tgagaagata gatggaaccg tggtcgatga tgcgcgccag acgtcggaag gtgggctggc
     4021 gggcgctatg gtacaagcac gaggcggaat aaagaaccac catggacagg ccgaagacgg
     4081 ttactcccaa caccgtggcg acggttccgt atgaggaagc tttgacgatg agcaacacgg
     4141 agccaagcag gcttgcaagg gcacccaggc cgtgtgtgat ggcgctggtg aattcttctc
     4201 cgggactatc cgggggattc aagggaacgc gtcgttccag ccattcggac gccgttggtt
     4261 tgggcagggt cggtttcggc acgggcgagt cataacacgg ccacgaaaga tgggggggag
     4321 agtcgtcgca ctttccctat ccacgtgatt taacaagtga tttttcgaaa ggggggcgcc
     4381 ttggaagggg aggaatgaaa acttatggtg aggtgagctt gacgagggtg gcgttcttgg
     4441 gtccgaggtc gatgagtcct tcatcggcga atacgatttt catggtacgc gtatcgatga
     4501 tcatattgaa gggcgatgcg cttttgtcga agaaggcgcc gagggttagt ttggggtcga
     4561 gcacgagggg gtattcgagt ttgaatcgtg cgccccaata ttcgatatcg gagaagtcgg
     4621 cgggctcgcc atcatcgtct tcaaaaatgg cggtgatgaa ttccacacgt ttttctttcc
     4681 aatacgggta atgcaccttg gagtccccgg cttctttttg gcagacggta caccagcggg
     4741 cggaagcatt gagcagcaga gccacgggtt tgttgggatc gttgtcggga ttgtagtaat
     4801 ccgacagtcg aatcgtgacg atcgtgtctt tcgtcgcctt gtattcggcc tcggagggat
     4861 tggcaaagcc cgaaaactta tagtttcgaa tgatggagcc gacgtcacga ccgaacggtc
     4921 cagcgggata ggggatcgtg gttccgggtt cgggctggtg caacgtaccc gggtcgattc
     4981 ctccgccggg atgttcttcc ccggcgacga tcgtaggatc ttcggtttcc gaggacgagc
     5041 atgcgaccat catgaggatc gacagcgaag cgagggcggc gcgcgtgaag gaaaacggca
     5101 tgaagtctcc tggggggcag cgtcgatttc ggggcatggg cgtagcatag acgtgaagca
     5161 aaacaaggca aggcgcaagc gtcgtggagc attcagttca ggaaatatgc gggagattga
     5221 gcgatatttt cggtctcggc cgcggagtgg agctggcttg gctcgaacgg gctcgcccgg
     5281 ggcggattct tgcgctatga aagagccggt cttccactgt agtcgaggta tcgatgaagt
     5341 tagccggtcg ttaccgtgcc ttcgcacgat gggtttgtat gctttccgcg agcgtgcttg
     5401 gctcagcctg tggctccgac ccggagccgg gaggaaccat ttgcgggggg gtaacgcatg
     5461 agggtcaatg cttgcagcgt tgtgaagact cggtttgcgt gcaaggcaac gcctgcgcct
     5521 tcaccgaaga tcatcccgaa ggggtttgta cgcttccttg tgacaacaac gatgcgtgcc
     5581 cttacggctt tgcctgttat tcgggcatcg cgaatgcgca aggagaggct ggtcaattct
     5641 gtctcgacct cggactgccc gagcagggca agccaggcgc ggcatgcacc tccaccgacg
     5701 actgcgattc gctgcatggt ttgtactgtt tcgaagccag gtgctcgttt ccctgtgagt
     5761 cgattttcga aacctgcccc gacgggttga tgtgcaaagg cgatccttcg ccggccgctg
     5821 gcaatccact tggttattgc gtaccggctc cctcggatat gggcgtggac ggtcgctatg
     5881 gcacgaactg tccgtttggg gatagtcaat gtgacgaggc agccgggttc gtatgcctgg
     5941 gcgcggaagg aaccgcggat gcgtattgct ccaaaccgga tggatgtcag caagatgccg
     6001 actgcccttc cggatattgg tgcggtgcca tccgcacgct gacggaaaaa gacatcgatt
     6061 tttccaaaca acccaaaacc tgcttgcgtc gaggcttctg ccatccgtgc gaatcagacc
     6121 tggattgcga atacgtcgac ggagcgatat gtgttccgga tacgcgcgga gaaaaattct
     6181 gctccacgcc gtgcgaattg gattcaaacg cctgcctcat cggcacttcc tgcgtcgacg
     6241 ggggcgatgg acgcacggca tgccgtcccg acgtgggtta ttgctatagc aaggatccga
     6301 aaggttgcga cccgtgtcgt atcgacccgg attgcggacc gaattccacc tgtgtctccg
     6361 gaaaatgggg ttataaacct accttgaatt ggtgctcgac gccctgtggg cccaacagcg
     6421 agcatggcaa agccacgtgc ccggtcgcac ccaacggcca ggagatgcta tgcctggacg
     6481 aaaatatgct gagtttggga gggcccttcg acgccgatga tcctgcgtat ttgtacaagc
     6541 attgttacgc gcctttcacg gtggataaca cggaattgta tccgggaaag gatccaccca
     6601 gtaacgtctg tggcaatgga agggtggagg ccggagaaga gtgtgacgat ggcaatagct
     6661 atgccggtga tggttgcgat caatgtaaag taacggctgc atgtacgttt acggtgcaag
     6721 agcccaacag tgatggcaat cccgtggtat cccccaagcc gagccatgtg aaatacgagg
     6781 gagaaaagct ggatccggca atgccctacg tgatatcgac agcggtctgt aagaccttca
     6841 aggtcgaagg ggcgttggag acggccggag acgtggatgt gattgcgatc atgcttccca
     6901 atgccgcgga aatgttcgtg gataccttca cgagcacacc gggaacttgt acggccgatc
     6961 tggtgaccga agcgcgagcc tggaaaaacg gcgagacgac ggaccatgaa aacctactcg
     7021 acctatccgt tccgtgtaag aagctatcgt cgttgcctga ggattacgct tcgacgggat
     7081 acgcttgtcc tggctcccct acccatcttg gctgcgggac ttgcgacggt cctggcgtct
     7141 gcggggtatg tgacgatgac agtggtcacg gggattgctc gcgaatgatg gtgagtacgg
     7201 ccacctcgtt cggtaactat ccaatttatt tcgatggccg ttacaaggtt cttcgggtct
     7261 atgcgaaaga ccctggagcg acggtaggca attatatttt gatcggatca cgttatagct
     7321 cacaggcgat gctgggccct tccacgccgg tcgtactcag ttgctattga aagcgatggc
     7381 gcgttccctt ctaccgcgat ccaagcgcac ccgaagctta ttccaacgac gaaccgctac
     7441 tccgatctcc tcgaaggctc gaattgtctt gggcgaagca gggggacggc tgcccacatc
     7501 gatattcttc cccgccaggt tcgaatagcg gccccccgga agcggggctc cccatgaaat
     7561 atctataatt tttcagtgat tagaacctat ggccccaccg atcctccaag ccttcgtcgc
     7621 actttatcac ctttcgtgat gactcagcag ggcttctctt ggttcaccca tgagggatcc
     7681 cggagtttgg tccatgaaac gttacggttg acctggaaag tatccccgtg agatattgaa
     7741 gcttgaggtt cttttcttct tttccaaggg tggtgccgca atgggttccc gcagaaggtt
     7801 ttttttattc gccgtggcga cgataagcgg attcttgtgg ggggtcgctt gcggttccga
     7861 cgatgacgga tccgatgctt ccgcgggtgc cggtgcttct gctggtgctt ctgcctctgg
     7921 cggccagggt ggttctggcg caagcgctca tcacgggggg aactcgggag agggggccgg
     7981 tggggaaagc acgggcggca gtggtggatc gaacgtgggg ggaagcggtg ccgagggtgg
     8041 gactggcgga acgcatcctg gcggaacctg tgagcccaat atcacaaaaa gctgtaattg
     8101 caccgaaggg gtcggagccc agcgatgcgc tcccgacggc acgggctggt ccgcctgcga
     8161 atgcgcggta tatggtgtcg aattggccgt cagccccaat ggcagcgata ctgcttccgg
     8221 cacgcctcag gaaccatttg ccacgctcgg tcgcgcgaag caggaagtca ccaaaatcgt
     8281 ggcgaatggg cttcccagcg gcggcgttgt cgtttggata cacggagggg attacgaatt
     8341 gaaagagcct ttgacgctcg gcgcgaccga gtccggttcc gaaaaggctc ccgtggtctg
     8401 gaggggtgtc cctggcgagt ctgctcgtat ccttggcggt gtgaaagtgc aaccgggcgc
     8461 gttttctccc atcccgtctt cctcgcctgt cgttgccaga ctcgactccg atgctcgtga
     8521 ccacgtacgg caactcgatc tttcgtcgca aggaatatcc gattttggag aactcgagcg
     8581 acggggattc tgtcgtcagg cttcccgctc tgccgtagaa ctcttcgtca atggcgttcg
     8641 attgccactg gcgcgctggc ccgacgctac ggccaacacg cttcccccgg ggctggagac
     8701 ggcagatgct ctcgatatat tcggttctcc cacgcccgat gtcactggcc actatactcg
     8761 catcggaaac caagacggcg tatcctcgtt tgctcgggac gggctcgtga atggcctcca
     8821 attccatcta tatcgtcata cgtgggatta ccagggcagc acgcacacgg cttggtttct
     8881 caccacgggt agttcgggtt acccaaccaa caacgaccct tggtggtaca aatacgcaca
     8941 cgagcttgga gcgatgtctc ctgccaccgg cgccggggct tccggaatcg tgaccacgcg
     9001 cgatccccag ggcatcaacc atggattcgc cagcattgcc gaggccctgt ccaaaacgca
     9061 atggcgctat tcgggagatc gtccttcccg ttggaccgac gtatccgact tacgttttca
     9121 cggcttctgg aaatacgcct gggcggattg ccacgtaaaa gcggcgagca tcgacacatc
     9181 cacccgaacg atcaccctgg gcgaggatac tggctacggc atcgaggaag gacagccctg
     9241 gtacgtgtat aatgtccccg aggaactcac ggaacctggg gaatggtggc tcgaccgatc
     9301 gacgggcctg ctctatgtat ggcctcccgc ggggttcgac acggctgatg tcctgttgtc
     9361 cacgttggcc gatgccatcg tgaacatcaa agacggctcc tggatcgaat tgcgggacct
     9421 gcgcatcgag gccggccggg cggaactcgt gaagctcacc gaaggaaccc atgtttccct
     9481 cgtcggactc accctgcacc acgccggcac caacgcggtt gtcatgtccg gcaccaatca
     9541 ccgcgtggat cactgttaca tagcgaatcc cggaagcggg ggcgtgcgcg tgagcggagg
     9601 agataggccc accctgctgg aagccgggaa cgtggtcgaa cattcgcgcg tggagaaata
     9661 tggccagtgg gagtggacct atcgacccgc cgtggacctt caaggcgtgg gccatatcgt
     9721 tcgacacaat ctcatgcgcg atgcgcctca ctccgctatc ctgtattccg gcaacgaaca
     9781 taccatcgag ttgaacgaga tccacggcgt ttgcgagtat tccagcgacg ccggcgcgat
     9841 atacgccgga agagattggg gagcacgcgg aaacatcatc cgccataatt tcctccatga
     9901 catccgtacg cacttcgaag gctacggtgt ccacggcgtg tatctcgacg attgtctcag
     9961 cggcattcgg gtcgaaggaa acgtgtttta caaagtctcc gggatggcca tccaacacgg
    10021 tggcgggcgt gacaatatcc tcgtcaacaa tgtcattgcg cgctgcggcc tggggctggg
    10081 tgcggacaaa cgtgctgtcg acttgctccc gaaaggaaaa cccaacaaca ccccggggga
    10141 tagttggaat ctcctcgaga aattgcagaa agtcggctat caggacgagc cctgggcatc
    10201 ccgttacccc gaatgtgccg cgattcccaa cgattggaac gccatcatca accctggggc
    10261 acaatggctt tacccggaag gatgcgtgtt ctcacgcaac gtgggattcg acaatagcag
    10321 ttgggtcaac gccggccagg gggtgctgga ggtctacaag gaatacgcaa acaacttgga
    10381 agacgtggat ccgttgttcg tcgacgaggc tcagttgaac ctggacttac aaccctcttc
    10441 cccagctttc gatattcctg gtttcgagcc gatccctttc cacgatatcg ggatcaaacc
    10501 gtagtccacc cgaagcgcgt tctttgtttg taattacaag gacttaacca cgtcccgcca
    10561 catctggcga ggatttctct cacggcccac gctcacggcc tacggcacac gcgaatcgct
    10621 cacggtcatc ccatgtcgat ccctgcagtg cgtccatccc atgttttgca gtctaacgtg
    10681 tcggagcaag ccaaaaccaa tcttcagggg atggtcgctc gtattccttt catattcaga
    10741 gaataatgag gggatctgtc agggagacaa cgattctcgg catctacgag ccttgggttc
    10801 cgcctgaggg cgtttcatca agacgccgtt gccagtcctg caattgctct tgcccgagcg
    10861 gctcgtatcg aagaagattg gggtcgcgta tcagagcatc ccaatcgtcg gcatcccaaa
    10921 tggtcgcaag aattctttcc aacgcttgtc ggacctcttc gtctgtggcg ccagtcatca
    10981 tcatgtttac caggttgtcg acctcgcccc ggcgctcggc ggggaggtgc tttttatatc
    11041 ggtaatagac cttccagtcg ttcatgctcc acaagttcat caagtagtgg aaatggagat
    11101 cgacaatggc ccgctcggag gaagaccacg ttcgccgtgc aacggggcct tgcggcaaag
    11161 tcggcagcac gctctttccc gtaccttttt tgacgcccct gacaacctcc gcgtccggta
    11221 cgatttctcg cagcagctcc tcggtgcagg ggaccggtag tggaatgacg aacggcaacc
    11281 ctaacgtttc ctcgagcccg tgcgagcgaa gccagctatc gcggccctga gattgacgca
    11341 ttttgcgggt gagttccttg atgagcgcgg acggcaaccg ttttcgttca gccatcccgt
    11401 ggaaaaagcg ttcatcctcc ggtccaaact cataggtgcc atccagagga acggtcaact
    11461 ccgcttgcca gccgtcgggt gcgaagatct ggagcacgac cgctgaatct tcatcgaacc
    11521 agacctggag gacggttacc gccaatagct gggatacacg tgccacgtcg agagaggctt
    11581 gatcgagcgg gacgaacgac gtccatcggc gattggatgc gagtatcgca caggagcctt
    11641 ctaacgtggc gagttttttc ttggtttgcg cgatactcag ttttcggaaa tggaggctgc
    11701 tcccctgctc gctcattcgc gcgtttatct catggaatcg cgatcttgcc aagggctaac
    11761 gataaatcgc caaaccatgg caccattgct aaagaatgcg cagggcacaa gtgtgtacgc
    11821 gctcagcgtg ccggatgtga ccccaagtcg tcagatgata tggtatcatg cgcaaacgag
    11881 gtattgggcg tcggacaagc gtcttccgac ggacgagaaa tggctctgta cggcgcaagt
    11941 cgccgtggac ccgggcagca atgcggcctt gggaaatagg aagtgcaaca ctacgggcta
    12001 aggccccgcc gacgcgaata aggcggttgg aaacgccatg gtggccagtt gtataagcga
    12061 ttggggagcc gaggatatgg ggggccattg gtgggagtgg acggacaagt ggtacgctgg
    12121 accaaacgcc accccgagtt gcatggacgc cgtgtcgtgg cccgcggcgg gcagctacgg
    12181 ctcggacggc acgtggtgca tttcgccagt gcgcaggatg gtcacacggg atgggtgcag
    12241 gggcttcctc cggctgcgct gcgcggcggc aactatggtg acggttccgc agccggcgtt
    12301 ttcagcatgt acctggccga agccgcctcg tcctagggaa aaagtagcgg attccggtgc
    12361 gtcgtcccca gccgatgatt ggatactcgt cgggcatccg catcgaagac caggtttccc
    12421 accaactccg agcgcgtgcc ccggcgccaa gggctcattt tcggatgagg ctttggccgg
    12481 gatggtaggg tttgggaagc ggacacgttc gcgcgttccc atcgcgcccg tcgacccagg
    12541 agttttccga ggttcgggag tcgaaattgg ggtgtcggga ccccatttgg ggctccgggg
    12601 agcctccttt gaagtatcgg aactccactt taggctccgg ggagcctcct ttgaagtttc
    12661 ggaactccac tttgggctcc ggggagcctc ctttagggtg tcgatacccc actttgggct
    12721 ccggggagcc tcctttggag tgtcgaaacc ccattttcaa ggtcgcaact caacccagtc
    12781 tgctcaacat tccacaaacc aacctcatca accccagtga aaagaggatg ccttcccgaa
    12841 caggacgaat cggtctgttg atttgggttg attgtttcgt ctcgttcatc gaaaatggga
    12901 acgttcgctc ggtg
//
LOCUS       DAOSOR010000097        12840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4873_length_12840_cov_4.157450, whole genome shotgun sequence.
ACCESSION   DAOSOR010000097 DAOSOR010000000
VERSION     DAOSOR010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 12840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12840
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4873_length_12840_cov_4.157450"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..424)
                     /locus_tag="PK710_07365"
     CDS             complement(47..424)
                     /locus_tag="PK710_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09570.1"
                     /translation="MQFTNPAVPDCTGHWLTKGIEGGSSITVAGQNKCPTCSCEPSAT
                     MYCGLYLGLYKTTTCTGDEFPIKMIADGKCKNYGLAAFERITFKRIEDMHIIEGSCTP
                     MVSGSPTFPPAQWDSPSRTCMRV"
     gene            complement(637..951)
                     /locus_tag="PK710_07370"
     CDS             complement(637..951)
                     /locus_tag="PK710_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09571.1"
                     /translation="MEHPPRIAPTDAARHNIVLGSRKLCDNRRLLSLSIGDCHSCACG
                     LLLLWVLLSRRWVALLTSMLPFLKHPRLRVVVEGEVEAATLAVMVAMPELVPQGELME
                     EV"
     gene            complement(1654..2184)
                     /locus_tag="PK710_07375"
     CDS             complement(1654..2184)
                     /locus_tag="PK710_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09572.1"
                     /translation="MPISSIVVWGTVYFPVSLVPPILVGSALVPILNAVRPGPGPLPP
                     TWLLGLALLFVFVYFVGWMPYVLWVKYRRSKRRRLLREGELIPATFVKCVKYLDKEAG
                     KFMDGKDVICLNCYYRFITDGKPRTTTFGEPNRSIVVLYHPSYRFGGVFVDGTLIYVK
                     KWHSDVQPDPLSLWEA"
     gene            complement(2573..3097)
                     /locus_tag="PK710_07380"
     CDS             complement(2573..3097)
                     /locus_tag="PK710_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09573.1"
                     /translation="MGSVVVQSSDFGSMRMTGPKRLDCYYAAWPFSVFLMFPTVVFIV
                     GSIAITVAAFKSGSPLPFLEPLLGLAWAGINVWVIARRRRIMGRFVVDADEGYLRRYR
                     GSRELESWPMTQVRFSTQWDPFHRGLRLEFGYHYWLVAEVPDGRKMRLGKGRTESLRP
                     VFELLRTWGLSTLP"
     gene            complement(3599..4879)
                     /locus_tag="PK710_07385"
     CDS             complement(3599..4879)
                     /locus_tag="PK710_07385"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HOT09574.1"
                     /translation="MPSYCPAVAFVCLTWFVGCGAGVIEQEKEGEIADAAVEAADAPL
                     DQGVQPSDAGTESSVSDSSQPDAKPAAPTEREVLIPLDGQVLFPAVVEGNPAYLVVDT
                     GAVRSTFSKDLLRNVANGVGLATIDFGDDIHFEDYEVLAGDLSEAEKHIGSAIDGLIG
                     QDLLQQLYFGVDYRRKRAIVAANAPANAPNGWTEADRITLPYELVQGLPVLSVDIEGK
                     QARLIADTGSGVTILTQSHVPSDWLDDGCKGYVWHTSYGSDEATIVRIPSLGVGSSQV
                     KNSWAIVVPDEHHLASLFSAIGLEVDGFLGYPVYRSFYWGIRGADQVYEVFAQPGQDP
                     LASTEWNRVGVEIARADNEVLIDMVFQPSDAFDKGIDVGDVLHAIDGTVVDTLSLDDI
                     RMRLRGSVGDARHLSFVRAGSSIEVDVAVESLIP"
     gene            complement(4909..5781)
                     /locus_tag="PK710_07390"
     CDS             complement(4909..5781)
                     /locus_tag="PK710_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09575.1"
                     /translation="MAKTISMGHHSCMKLLCRTFFALALSFGIGCGSDEPGDSSHAEA
                     GSSGSSGSAGSSSTETPDSGDESEPIDSGLADQQNEDTTTVTDVSEDTHPVDGQVDVE
                     PVEYTSLGTLTVDCQVPFVLDASQVTSMPYMTSHFGHLIQQYGITGIIDGQDITAFPE
                     KMYYGAHMPAHLQPNDLLSLVQISMASVTQPTYSVRIDFRPDTAVTTGSTWNVGLEEN
                     QAVAALFKHISTNQLCLMSMGIGGKLTFQSASNTTQVEGGSFHVVGTMNMVAPEEVPD
                     LCDMAAQLNTPCCP"
     gene            5893..7926
                     /locus_tag="PK710_07395"
     CDS             5893..7926
                     /locus_tag="PK710_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09576.1"
                     /translation="MRWPLLGIAWIAAVSGCGGGSGSSEAVQVAVSSTNSCATFRDGT
                     VRCWGSALAQRGGGVTPSYLPGKYRLELRLSEEELRTPNKVVNLKDVVETVVSSQHSC
                     SRLKNGQVRCWGKVPRRRDVVPYPIAEPEVSDAAQIALMDESTCIRHTNGTVSCVGQV
                     EFAGLSGVTDLQARGDLACAIVSPGTVQCWGNSSKGERGDGVSAQQISLPTPTVIKRK
                     PGGQWFLGRTISCHLSSSGQAQCWNSTTSEMVFATSAQDVLQSFAMSDSGSCGVLSNH
                     TLRCWNYAWQPTDKKGMLDSPQQIVVSGDHQCARLASGQVQCWGDSWRGTSEDALRQD
                     GPWLVEGLGKAVDIGSNHDLTCAVVEDGSVRCWQASRQLKTMEGVTDAVQVSMGVKHT
                     CVRQARGTVVCWGFGDRGQLGREGESETWASQPQKPEQVVGVTGAEQIVVGDDTTCVR
                     LTNGAVQCWGDNRRGQLGQGYFSEEGTKSKSATPQFVSLPRPAVDLYGYARTFCAKLT
                     DGNTACWGINVGLVEAKKIPPTQPTAVVGITDALQVAVAPSHACARTKAEKVYCWGSN
                     EKGQMGDGTWGEGNIRAEPVEIALLRGASEIWVSLGATCGRRQDRRVICVGMSQQTPW
                     ELPGLVTQAAQIALSYGQSPLFSPPPYPGCAVMNNGSLACWDFGVTQPTQVRW"
     gene            complement(8483..12265)
                     /locus_tag="PK710_07400"
     CDS             complement(8483..12265)
                     /locus_tag="PK710_07400"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HOT09577.1"
                     /translation="MTLPRFAQWLSLLLAVALLGGFVQPSHAQTNEPIDSRVVEATRD
                     IVAARGPDRYTKLRVLWSLWEDTDPDQVEQALRSMQRHPSLDPSARVYASLLSAYARR
                     RRGDLPSARAQVSALRFIDQWMVAGPFDNEGRSGHDRVFEPESRLNDPVDPQLVYEGK
                     ERQVRWRAIPHAFPDGWLDFGELFRPQERICGFATTFVRARDDKARVATVWLGATGAF
                     RLFWNGQEVLSDGAYRTMDAERFATTVDVKAGWNRLTVKVCGDALSPMLTVRLGDRQG
                     APDASLVVSADAVHADDAAKNAIHAPSPNQARITIRAQGAEGSIRAANAPARRERDPG
                     QVRGAIDRFEAMTRGKATPEALEAHARYLMITGGDDPSMHQARDFAIRAAEKAPTIER
                     LLLASSLVEDRNQQRTWIDKAVATAGNDPSLELLLAQARLARGGPHWRDAVPFYDRAL
                     AKNPDCVEALLGRVDLYNEAGLRRTALGMLERAIERHPHAVGLLRVYALQLADLDRLS
                     EAQQVSARYMAYRFDDVTQQATRLDLALAQRNHELAQHWINRLLATEPGSVHRLNDVA
                     RAYTNMNMSDKAIQAYQRALELAPEDTDTLRELAELYGRLGQSEQQLRILRHVLALRP
                     QLKAVRDLVEHLEPKQVRADEAYAWSSERFLPLRTEASGGFNQRTLRDLQVTTVYPSG
                     LSSAFRQVVFQPLTEEAATAARDYAFAYQADRQIVQLGTARVYRADGRIDETIETSEG
                     PADNPAIAMYTSARIFYVHLPRLEVGDVIELRYRIEEVSQENVFADYFGEVQPLQSSE
                     PMRNAEYVVIAPTSRKLIFDVTPLEGLQREEKEQGDVRITRFFVPSVRPILSEPNMPP
                     WSEILGHVHVSTYASWKDVGAWYWGLAKEQIHPDDEVRRKALELTRGLETNEQKVRAI
                     YGWVVKRTRYVALEFGIYGYKPRRASQTLARGWGDCKDKATLIVSMLQVVGIPAHLVI
                     VRTGLRGNFPSQPASLAPFDHAIAYVPSLDLYLDGTAEFSGSTELPGMDRGSMALVVD
                     PEQSRLVTLPDPSAQQTIRKKHIRATLRADGTAHITIRHEVSGAIAAQWRLRYHGETS
                     LRDRIGADTAQGLPGFVLDPGNAGVEAADLENIEQPVRLRVSGKTQSFGRKQGDELSI
                     PVTPSDRYVPRFASLSQRKLDVVLPYRSTVSETWTIELPAEVKVQELPKPMALTTPFG
                     ALRLEVRHTGNVVEVDTNISLDKLRIAPNEYAAFAAFCEQIDRALQQRLMVTRKQP"
     gene            12503..>12840
                     /locus_tag="PK710_07405"
     CDS             12503..>12840
                     /locus_tag="PK710_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09578.1"
                     /translation="MKNKSLWMVSAALVVGAVVVGCIVEQRPAQQPPPGQPPPGQPAA
                     APPQAAPPGPAAQPAQPQPQPAPPPGGAQPQPGQPAATATGIPGLPPRFPPPPRRPPP
                     PPRAPPPPPPP"
BASE COUNT         2835 a         3731 c         3708 g         2566 t
ORIGIN      
        1 gttccaagcc tcttcccatc gaggtgctgt ctgaccgggt aatggatcat acccgcatac
       61 aggttctgga gggagagtcc cactgggcgg gtggaaacgt gggcgaaccc gataccatgg
      121 gagtacaaga gccctcgatg atgtgcatgt cctcgatgcg tttgaaggtt atgcgttcaa
      181 aagccgctaa tccgtagttt ttgcacttgc catctgcgat catcttgatc ggaaattcat
      241 cgccagtgca ggtggttgtc ttgtaaagcc ccagatacaa accgcaatac atcgtagcgc
      301 tcggttcgca actacatgtg ggacacttat tctgtccagc gaccgtgata gaggaaccgc
      361 cttcgatgcc cttcgtaagc cagtggccgg tgcagtcagg aaccgcggga ttggtgaatt
      421 gcacgggacc attccacggt gcaggaggtt tgggcaggca ccgataaccg aatgaagcgc
      481 actggggatc ctggcaatcg accaacccat ccccgttgtc gtccttgttg ttgtcacata
      541 tctcgtttga tgaaccaccg gttgcgcccg tgcctcccgt gggtcccgaa cccccggtgg
      601 cagccgcacc acctgttcct cctgttgacg ctcctcctaa acctcctcca tcagttcccc
      661 ctgcggcacc aattccggca tcgccaccat gaccgccagg gttgccgcct ccacttctcc
      721 ctcgaccacc acccgaagac gcgggtgctt cagaaaaggc agcatcgaag tcaatagtgc
      781 aacccatcgg cgagacaaga gcacccacag caacaacaac ccacaagcgc atgaatgaca
      841 gtccccaatc gacaaagaca acagccttct attatcgcac agttttcgtg agcccagaac
      901 gatattgtga cgcgccgcat ccgtaggagc aatccttggg gggtgttcca ttcgctttcc
      961 tcacgcaagg cgcacaaagg ctgccgatgc tgaatcattc cctcattttt ccccgagcat
     1021 ttccttcacg ctcacggtca cggcgcacgg cacacaaaga acgctcacgg taacggtcac
     1081 tcgcatagcg ttcaagcaac cgtatttatt gatgtttttg taaggccgat caaaatggaa
     1141 gcaaggacca ggtcaaactc aaagtccaag gaatcccctg ctcgtatccc tttccaattc
     1201 aagaaaaatg agggaatcta tcagcaatgc tgaggggacc agcgccattt ctcgcgcatc
     1261 tggcgactcc gatccccctc cgcgtcaccc gtttggccgg tacgtgggcg tgctggcaag
     1321 cgcgtcgcga tgacgtgaac gtgtggtgcc aaaaaccaag ggctccaaac accggccaca
     1381 acgtccgagc gaaccacaag cgccgtcgtt gaccaaaacc acggaagtga cgtcttcgct
     1441 aacggctttg gctgaaatcg cgaaacccga aacgccgcga agccatcgcg gacccgtggt
     1501 ggctaccggt tcgtacgtga actggatgac cttttttcgc cgagtgttcc ccggggattc
     1561 tacgtcatgt cccgcgccgc ggcgggagac ctcgtgtcgt caccccggtc ctacgatggt
     1621 tggaatctta aaaagcttag tgagaacgcc gggttatgct tcccataggg acaggggatc
     1681 gggttgaacg tcgctatgcc actttttcac gtagatcagg gttccatcca cgaagacccc
     1741 accaaaccta taactgggat ggtagagcac cacgatggat cggttgggct ccccgaacgt
     1801 ggtggtgcgt ggtttgccat ccgtgatgaa gcggtagtag cagttaagac aaataacgtc
     1861 tttgccgtcc ataaacttgc cggcttcttt atcgagatac ttgacgcatt tgacgaaggt
     1921 tgcgggaatg agttctcctt cacgaagcag tcgacgacgt ttgctgcgcc ggtatttgac
     1981 ccataggacg tagggcatcc atccgacgaa atagacgaaa acgaaaagga gggcgaggcc
     2041 aagcagccac gttgggggca gggggcctgg gccgggacgg acagcgttga gaatcggcac
     2101 cagggctgaa ccaacgagga tgggaggaac gagggacact gggaaataga ctgtacccca
     2161 cacgacgatt gagctgatgg gcacgaacct tcggacaaaa ggctcacata gagctttgct
     2221 ccgaggtttg tcctcgcctt gaaaccgttc caaatcggtc attttcgaac aggataggtc
     2281 gtcttccatg ttttcgtcca ctttcgagac cgaagggccc gcctgaaggt agctcccgaa
     2341 aacgacggtg ccaccagatg ccgttccggg gggcatgtgg tgcttacacg cgcatatatc
     2401 gtcattacgt tggaaaaatg gccgatgcct cgcccccgga caacaacggc cagctacgta
     2461 tcgtgatcga gatcgactag gactggcccg gttccgggcg tgacggcaac cgaccagggg
     2521 cggtggaacc tctcgccgcc atggctggac acatcgttat ctgcggcgac ggtcagggga
     2581 gtgtgctcaa tccccacgtg cgcagcagct cgaataccgg gcgcaaggac tcagtgcgtc
     2641 cctttccgag ccgcatcttg cgcccatcgg gcacttcggc gacaagccag taatggtacc
     2701 cgaattcgag cctgagcccg cggtggaacg gatcccactg ggtggaaaac cgcacctgcg
     2761 tcatcggcca cgactccagc tctcggctgc cccggtaccg tcggaggtaa ccttcgtcgg
     2821 cgtcgacgac gaagcgcccc atgatgcgcc ggcggcgtgc gatcacccag acgtttatgc
     2881 cagcccatgc cagcccgaga aggggttcca ggaacggaag tggtgagccg cttttgaacg
     2941 cagccactgt gatcgcgatc gaccccacga tgaacaccac ggtcggaaac atcaggaaaa
     3001 cgctgaacgg ccaggctgcg taatagcaat ccaaacgctt gggtccggtc attcgcatcg
     3061 aaccgaaatc gctgctctgg acgacaacgc ttcccatgcg cgtcatctta ccgtccgcgg
     3121 cacccgtcta ccaagcgaat gacgagttcg aggcaggtga tcgagcgcta tgctacaggt
     3181 gtcccccagg gccggtgtcc acaccaacct atcgatggca acagggtcgg tgtacgggcc
     3241 caatcctccc gacaaatacg ttcagcgtcg gaacatgctt tcccttcgta cccatactcc
     3301 ctcgtagagg acaccgtaga ctcatccact ctcgaaccca ccgctgcacg ccatattcga
     3361 cgaatgggtt gcggccatgc acggatggct gctccatcgc atatcgacaa gccgtgaagg
     3421 cactcggctg ccgtattgcc cctacccatt ttcaatgcac gtaacgccgt tgccaaaccc
     3481 cacgatggct cggtgggccg aaacacgaaa tcgctcaaca aagcgattgt gccctaagaa
     3541 agcggactgt ccaacgagac tctgcctttt tgtttatgct gtaactattt gaaaatattc
     3601 aaggaataag agattcgacc gccacgtcca cctcgatgga tgagcccgcg cgcacaaaac
     3661 tcaaatgacg agcatcaccc acgcttccac gaagacgcat tcgtatatcg tcgagggaga
     3721 gagtatcgac gaccgtgcca tcgatggcat gaagcacgtc gccaacgtca atccctttgt
     3781 cgaaggcatc ggatggttgg aacaccatat cgatgagcac ttcattgtcc gcgcgagcga
     3841 tttccacacc tacacggttc cattccgtgg atgccaacgg gtcctgtccg ggttgagcaa
     3901 aaacctcata gacctggtcg gcgccgcgaa tcccccaata aaacgatcga tacaccgggt
     3961 aaccaagaaa gccgtcgacc tcgagaccta tggcagagaa caacgaggca agatggtgtt
     4021 catccggaac gacgatcgcc catgagtttt ttacctggga gcttccgact ccgagcgatg
     4081 ggatgcgcac gatcgtggct tcatccgaac catagctcgt atgccataca tagcctttgc
     4141 atccgtcgtc gagccaatcg gaggggacat gagattgggt gagtatggtg acgccggagc
     4201 cggtatccgc gatgagtcgt gcttgtttgc cttcgatatc cacggaaagc acgggcagac
     4261 cttgcaccaa ttcgtagggc agcgtgatgc gatcggcttc ggtccagccg ttcggtgcgt
     4321 tcgcgggagc gtttgccgcc acgatggcgc gctttcgacg ataatccacc ccgaaataaa
     4381 gctgttgcaa cagatcttgt ccgatcaggc catcgatggc tgaaccgatg tgtttttccg
     4441 cttcggaaag atcccccgcg aggacttcat agtcctcgaa atgaatgtca tcgccgaaat
     4501 cgatggtggc aagacccact ccattggcca cgtttcgcag cagatctttg gaaaacgtcg
     4561 agcgaacggc gcccgtatcc accaccagat acgcagggtt tccttccaca accgcgggaa
     4621 aaagcacttg gccgtcgagc gggatcaaca cctctcgttc ggtaggcgca gcgggtttgg
     4681 catccggttg tgaagaatcc gagacggaag attccgtccc cgcatccgag ggctgaactc
     4741 cctgatcaag cggagcgtcg gcggcttcca cggcagcatc cgcgatttct ccttcttttt
     4801 cttgctcgat caccccggct ccacagccga caaaccaagt caggcacacg aaggctacag
     4861 ccggacaata agaaggcatg gcacgctccg ttcgagattc gctcgagatc aagggcagca
     4921 aggcgtgttg agttgggcag ccatatcaca aagatcgggg acttcttctg gtgccaccat
     4981 gttcatggtg cccacgacat ggaacgaacc accttccact tgtgtggtgt tgctggcact
     5041 ttggaacgtg agttttccac cgatgcccat gctcatcaaa cacagttggt tggtggaaat
     5101 atgcttgaac aaggctgcga cggcctggtt ctcctccagt ccaacgttcc acgtcgaacc
     5161 cgtggtgacc gcggtatcgg gtcggaagtc gatgcgaacg gaatacgtgg gctgggtcac
     5221 actcgccatg ctgatttgca cgagcgagag cagatcgttg ggctgcagat gagccggcat
     5281 atgggctccg taatacattt tttcgggaaa cgcggtgatg tcctggccgt cgatgatgcc
     5341 cgtgatgcca tattgttgaa tcaaatggcc gaaatgcgac gtcatataag gcatgctcgt
     5401 gacctggctg gcgtccaata cgaacggcac ctgacagtct acggttagcg ttcccagcga
     5461 tgtgtattcg accggttcga cgtcgacctg tccgtcgacg ggatgggtgt cttcggatac
     5521 gtccgtgacg gtggtggtgt cttcgttttg ctggtcggca agtccggagt cgatgggttc
     5581 ggattcgtcg ccggaatctg gtgtttctgt gctggacgag cccgcggagc cggaagaacc
     5641 ggacgagcca gcttctgcgt gggatgaatc gccgggttca tcgctaccgc aaccgatccc
     5701 gaagctgagg gcaagtgcga aaaaggttcg gcaaagaagt ttcatgcacg agtgatgacc
     5761 catcgagatg gttttggcca tgcataaaag gaaaagtgca tggcgaagag cgtgaccgcc
     5821 ggtgtcgttc ggggcttgca cggcaggaag gtgtgattga cagctttttg gcagggcgcg
     5881 tacacttggg ggatgcgatg gcctttgctg ggaattgcct ggattgctgc cgtttccggt
     5941 tgtggaggag gatctgggtc ttcggaggct gtccaagtcg ccgtgagttc aacgaattct
     6001 tgcgccacgt ttcgtgatgg gacggtgcga tgctggggca gcgcacttgc gcagcgtggt
     6061 ggaggtgtaa cgccgagcta tctgccagga aaatatagac tggagttgcg cctgtccgag
     6121 gaggaactcc gaacccccaa caaggtggtc aatctaaaag acgtggtgga aaccgtcgtt
     6181 tcttcacaac attcctgcag ccggttgaaa aatgggcaag tgcggtgttg gggaaaggtg
     6241 cctcgacgac gggacgtcgt cccttatccc attgcggaac cagaggtatc ggacgcggcg
     6301 cagatcgctc tgatggacga aagcacctgc atccgtcaca ccaacggtac ggtgtcctgc
     6361 gtcggtcaag ttgagtttgc gggactgagt ggtgtaacgg atctgcaagc caggggggat
     6421 ttggcctgcg ccattgtctc gccggggacc gtgcaatgct ggggaaacag cagcaaagga
     6481 gagcgaggtg atggggtctc cgcacagcag atctcgttgc caacgcctac ggtaatcaag
     6541 cgcaagccag ggggacagtg gtttttggga cgtaccattt cctgccactt gtcttcttca
     6601 ggccaagctc aatgctggaa ctcgacaacg tcggagatgg tatttgcaac ctctgcgcaa
     6661 gacgtgctgc agtcctttgc gatgagcgac agcggatcct gcggggtatt atcgaaccac
     6721 acgttgaggt gttggaatta cgcgtggcag cccacggaca aaaaaggaat gctggattct
     6781 ccccaacaaa tcgtcgtgag cggtgatcat caatgtgccc gattggcttc cggacaagtc
     6841 caatgctggg gagattcctg gcgtggcact tccgaggatg ctttacgaca agatggccct
     6901 tggctcgtcg agggtcttgg aaaagctgtg gatattggct cgaatcatga cctcacctgc
     6961 gccgttgtcg aagacggtag cgtgcgatgc tggcaagctt cccgccaact caaaacgatg
     7021 gagggagtca ccgatgccgt gcaagtgagc atgggagtga aacacacgtg cgttcgtcaa
     7081 gccagaggta cggtcgtatg ttggggattc ggagaccgtg ggcagctcgg acgtgagggg
     7141 gaatcagaga catgggcatc acaaccgcaa aaaccagagc aagtcgtggg agtgacagga
     7201 gctgagcaga ttgtggtggg cgacgacaca acgtgtgttc gtctcaccaa tggtgccgtg
     7261 cagtgttggg gggacaatcg acgcggtcag cttgggcaag ggtatttcag cgaagaaggg
     7321 acgaaaagca aaagcgcgac gccccaattc gtttccttgc ctcgccccgc tgtagatctg
     7381 tacggatacg ccagaacgtt ttgcgccaag ctcacggatg gcaacacggc ctgttgggga
     7441 atcaacgtgg gcttggtgga ggcgaaaaag attcctccta cccaacccac ggctgtcgtc
     7501 ggaatcaccg atgcgctgca ggtggccgtc gctccctctc acgcctgcgc tcgaaccaaa
     7561 gcggaaaagg tctattgttg gggcagcaac gagaagggac agatggggga cgggacgtgg
     7621 ggcgagggaa atatccgtgc cgagcctgtc gagatagcgt tgttgagagg agccagtgag
     7681 atctgggtct ctttgggggc aacgtgtgga cgaaggcaag acaggcgggt gatatgtgtg
     7741 ggaatgagcc agcaaacgcc gtgggagctt ccggggttgg tgacccaagc ggcacaaatc
     7801 gctctttcgt acggacaatc gccattattt agcccccccc cctatcccgg ctgcgccgtg
     7861 atgaacaacg gttctttggc atgctgggac ttcggtgtta cccagcccac gcaagtacgt
     7921 tggtgagtct caggcacggc tcacggtcac ggccacggcc cacgaaaccg gtcacggtca
     7981 cggtcacggc ccacgagaaa aaagatgccg acgcgccggt ggcattcctg tttaccgcac
     8041 cctcaggaaa aaataaggga atctgtcagg gatatcccct gcacgaaccc ggccctagat
     8101 atggaaataa ttagtaattt caatagcata caataatcag aaacctggtt tcaagggtca
     8161 accccctcct cacaggaatg atgtcgttgg catctgacag atcccctcat ttttgccgaa
     8221 ttcgaaaggg ttacgagctg gggttttgtg cgccgtgcgc cgttttcgtg ggccgtggtc
     8281 gtgaagccag ggcaacaacc ggaaccgtag gggcgggttt caaacccgcc cgcccatgcg
     8341 cgattggcga gatgttgaaa tcccccgggc aaaacgaggg gatggcgtag cgttggcgtc
     8401 agtccttgtc cgatgcgtga aaagatgtcg acgggtagaa ggaacgatcc caaatcacca
     8461 gcgggcaaga ggcaaccaaa cctcaaggct gcttgcgcgt caccatcagc cgctgctgca
     8521 atgcccggtc gatttgttcg caaaacgcgg caaacgcggc atattcgttg ggcgcaatac
     8581 gaagcttgtc caggctgata ttcgtatcga cctccaccac gttgcccgta tgcctgacct
     8641 cgaggcgaag tgcgccgaaa ggtgtcgtca acgccatggg tttgggcaac tcttggactt
     8701 tcacttccgc cggtaattcg atggtccacg tttcggaaac cgtggaccga tacggaagca
     8761 ccacatcgag cttgcgttga gacaacgaag cgaaacgagg cacgtaacga tccgaaggag
     8821 tgaccggaat ggatagctca tcgccctgtt ttcgtccgaa gctctgggtt tttcccgaga
     8881 ctcgcagacg caccggttgc tcgatatttt cgagatccgc tgcctcgact cccgcgttgc
     8941 ccggatccaa tacgaaacca ggcaagccct gcgccgtatc cgctccaatg cggtcgcgca
     9001 gagacgtttc accgtgatac cgcaaacgcc attgcgcggc gatcgcgccg gatacctcgt
     9061 gccgaatcgt gatatgggcg gtgccatccg ctcgcaacgt ggcgcgaata tgtttttttc
     9121 ggatggtctg ctgggcggaa ggatcgggaa gagtgacgag ccgggattgc tccggatcga
     9181 ctacgagagc catgctgccc cgatccatgc cgggaagctc cgtcgacccc gaaaactccg
     9241 ccgtcccatc gagatacagg tcgagcgatg gcacgtacgc aatggcatgg tcgaaaggcg
     9301 cgaggctcgc gggttgcgac ggaaaattgc cgcgaagtcc ggtgcgtacg attaccaaat
     9361 gagctggaat tccaacgact tgcagcatgc tcacgatgag cgtggccttg tccttacaat
     9421 ctccccaacc acgcgccagc gtttgagaag cacgtcgtgg tttataaccg taaatcccga
     9481 actccagcgc cacgtatcgc gttcgcttga ccacccaacc ataaatggct cgtaccttct
     9541 gttcgttggt ctcgagcccc cgcgtcaact cgagcgcctt tcgtcgcacc tcatcatccg
     9601 gatgaatctg ctctttcgcc agcccccagt accaggcacc cacgtctttc caggaagcgt
     9661 aggtggatac atgcacatga ccaaggattt cggaccatgg cggcatgttc ggttcggata
     9721 aaatcgggcg aacggaagga acgaaaaagc gcgtgattcg gacgtcacct tgctcctttt
     9781 cttcgcgctg caaaccttcc aaaggcgtga cgtcgaagat caatttgcgt gacgtggggg
     9841 caatgaccac gtattcggcg ttgcgcatgg gttcggaaga ctgcaaaggc tgcacttcac
     9901 cgaagtagtc cgcaaacacg ttctcctgcg atacctcttc gatgcggtaa cgcaattcga
     9961 tcacatcgcc tacctcgaga cggggaaggt gaacgtaaaa gatgcgggcg gacgtataca
    10021 tcgcaatggc cgggttgtcc gctggcccct cgctcgtctc gatggtttcg tcgattcgcc
    10081 catccgcccg gtacacacgc gccgtgccga gctgaacgat ttgccggtcg gcttgatagg
    10141 cgaaagcgta gtcccgtgcc gcggtggccg cttcttccgt aaggggctga aacactacct
    10201 gccgaaaggc gctcgacaat ccactgggat acaccgtggt gacttgcaaa tcccgcaagg
    10261 ttcgctggtt gaatcctccc gaagcctccg tgcgtagagg caaaaaacgt tcggaagacc
    10321 aggcgtaggc ctcgtcggct cgcacctgct tgggctccaa atgctcgacc agatcccgca
    10381 cagctttgag ctgcggacgc aacgccagca cgtgtcgaag aatgcgcaat tgctgctcgc
    10441 tctggcccag acgaccatat aattccgcaa gttctcgaag ggtgtccgta tcctcgggtg
    10501 ccagctccaa cgctcgttgg tatgcctgta tggctttgtc cgacatgttc atgttcgtgt
    10561 aggcacgcgc tacgtcgttc agacgatgaa ccgagccggg ttccgtagcc aaaagccgat
    10621 tgatccaatg ctgtgccagc tcatggtttc gctgtgccaa cgcaaggtcc agacgagtcg
    10681 cctgctgcgt gacatcgtcg aaacggtacg ccatataacg agctgatacc tgctgagctt
    10741 cggagagccg gtcgaggtcg gcgagttgca aagcgtatac gcgcagcagt cccaccgcgt
    10801 gaggatggcg ttcgatcgct cgttcgagca ttccaagagc cgtgcgacgc aaacccgctt
    10861 cgttgtacag atcgacccga ccaagaagag cttcgacaca atcaggattt tttgcgagag
    10921 cgcgatcgta gaacggaacg gcatcgcgcc aatgcgggcc accgcgcgca agtcgcgcct
    10981 gggccagcaa caattccaaa gaaggatcgt tgcccgccgt cgccaccgcc ttgtcgatcc
    11041 aggttcgttg ctggttacgg tcttccacaa gcgaggaggc aagcaatagg cgctcgatcg
    11101 ttggcgcttt ctctgccgcg cggatcgcaa aatcgcgggc ttgatgcatc gatggatcgt
    11161 ctccccccgt gatcatgaga taacgagcat gggcttcgag cgcttcgggc gtggctttgc
    11221 cccgcgtcat ggcctcgaaa cgatcaatgg caccgcgtac ctggccggga tcccgctcgc
    11281 gacgcgccgg tgcattggcc gcacgaatcg acccctccgc tccttgcgca cgaatcgtga
    11341 tacgggcttg gttcggcgac ggcgcatgaa tcgcattttt tgccgcgtcg tccgcatgga
    11401 ctgcatcagc agacacaacg agcgatgcat cgggtgcgcc ctggcgatcc cccaaacgaa
    11461 ccgtcagcat cggtgataag gcgtcgccac ataccttcac cgtcagtcgg ttccatccgg
    11521 cttttacatc taccgtggtg gcgaaacgct ccgcatccat cgtgcgataa gctccgtcgg
    11581 ataaaacctc ttgcccgttc caaaaaagcc ggaatgctcc cgtggcaccc agccaaaccg
    11641 tcgctacccg cgccttgtca tcgcgagcgc gaacgaatgt cgtggcaaag ccgcaaatgc
    11701 gttcctgagg acgaaataac tcaccgaagt cgagccaacc atctggaaag gcgtggggga
    11761 tggcgcgcca gcgtacttgg cgctccttac cctcgtacac gagttgggga tcgaccggat
    11821 cgttcaaccg actctcgggc tcgaagaccc gatcatgacc agaacggcct tcgttgtcga
    11881 aaggacctgc caccatccac tggtcaatga atcgcaaggc ggacacttgc gcgcgggccg
    11941 aaggtagatc gccccggcgc cttctcgcat aagcagaaag cagcgatgca tagacgcgcg
    12001 cggaagggtc caggctcgga tgtcgttgca tcgagcgtag cgcctgttcg acttgatccg
    12061 ggtccgtgtc ctcccataag gaccaaagta cacgaagctt cgtgtaacgg tccgggccac
    12121 gagcggccac aatgtcgcgg gtggcctcga ccacacggga gtcaatgggc tcgttggtct
    12181 gcgcgtggga aggttgtacg aagccgccaa ggagtgcgac agcgagcaga agggatagcc
    12241 attgggcgaa tcgaggcaag gtcacggcca gagggtttct cgccaggctt gtgcggtcaa
    12301 gccttctctt cgtgtcggat gcaaacggta cgaaatgggg aggttccggc ggggcagaca
    12361 gagcccccga atcgaacatg aaccgaaaag gagccttcga tggcagcgat accgccgtgt
    12421 atcgtgtatc actgcttcaa cccgtgggaa gaatcggcta ccagccgtgg tatcatgcat
    12481 caccaaccca gaggtggtgc ccatgaaaaa caaaagctta tggatggttt ccgcagcgct
    12541 cgtcgtaggc gccgtggtcg tagggtgcat cgtcgaacag cgtccagcac aacaacctcc
    12601 gcctggacag cccccgcccg gacaaccggc cgcggcacca ccgcaagccg cacccccagg
    12661 cccggctgca caaccggcgc aaccccaacc ccaacccgct ccacccccag gcggcgcgca
    12721 accgcaacca ggtcaacccg ccgccaccgc caccgggatt ccaggtcttc ccccccggtt
    12781 tccaccccct ccccggcggc cgcccccccc cccgcgggca cccccccccc cccccccccc
//
LOCUS       DAOSOR010000098        12743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4919_length_12743_cov_5.438603, whole genome shotgun sequence.
ACCESSION   DAOSOR010000098 DAOSOR010000000
VERSION     DAOSOR010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 12743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12743
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4919_length_12743_cov_5.438603"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /gene="rplQ"
                     /locus_tag="PK710_07410"
     CDS             complement(<1..413)
                     /gene="rplQ"
                     /locus_tag="PK710_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOT09579.1"
                     /translation="MRHLKAGRKFGRNPSSRRAMLRNLTANLITHERIETTDAKAKEL
                     RRVAERLITKAIRLGDVAYNKPGETPDRRQIARRLHVARQIRAFIPRYGMVANEEGAM
                     QRIDLVEKVLIDLAKRFQGRPGGYTRIIKLGPRRG"
     gene            complement(432..1454)
                     /locus_tag="PK710_07415"
     CDS             complement(432..1454)
                     /locus_tag="PK710_07415"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOT09580.1"
                     /translation="MEQLIARNWRELIRPKAIQIDAESRSDVYAKFTCEPLERGFGIT
                     VGNSLRRVLLSSLQGAAVTAVRIDGALHEFTTIRDVVEDVSDIILNIKEVVFKAHRLD
                     NFVVRIDKEGPGPVCAKDIHLVDGLEVLNPDHVIAVLDKKGPLSMELTVGMGRGYVPA
                     ERNKTATMPLGTIPIDALFSPVRKVNYTVQNARVGQVTDYDKLVLEVWTNGAVRPEDA
                     VAFAAKILKEQLTIFINFEETEETSYVAPSDSEEPINENLFRSVDELELSVRSANCMQ
                     NANITLIGELVQRTDQDMLKTKNFGRKSLKEIKEILASMGLSLGMKIDNWPQLLERYK
                     AQQSQN"
     gene            complement(1558..2187)
                     /gene="rpsD"
                     /locus_tag="PK710_07420"
     CDS             complement(1558..2187)
                     /gene="rpsD"
                     /locus_tag="PK710_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOT09581.1"
                     /translation="MARYIGPVCKLCRREGEKLFLKGERCQTEKCAFTRRSYPPGQHG
                     QGRIKLSEYAIRLREKQKVRRVYGVQERQFRLYYDRANRMRGGTGEQMLALLERRLDS
                     AVYRMGFGVTRREARQLVKHNHILVNGRRVNIPSYILRIGDKVEVRERSRKLKLLESS
                     IAMAEARPVPGWMDLDRGSYSAVIKSSPLREELNEPAIREQLIVEYYSR"
     gene            complement(2206..2610)
                     /gene="rpsK"
                     /locus_tag="PK710_07425"
     CDS             complement(2206..2610)
                     /gene="rpsK"
                     /locus_tag="PK710_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOT09582.1"
                     /translation="MSTTKARGKGVKQKKKVKKNIGSGIAHIQSTFNNTIVTISDVNG
                     NVVSWSSAGSRGFKGSRKSTPFAAQLAAEEAARRAMEHGVRSVAVFVKGPGAGRESAL
                     RALQSAGFKVTLIRDVTPIPHNGCRPPKRRRV"
     gene            complement(2626..3006)
                     /gene="rpsM"
                     /locus_tag="PK710_07430"
     CDS             complement(2626..3006)
                     /gene="rpsM"
                     /locus_tag="PK710_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOT09583.1"
                     /translation="MARIAGVDLPRRKHVVYALPYIYGIGRKLARDICEKAQVSPSKR
                     VEDLAESEIKAIREIIDGEYKVEGDLRREVQMNIKRLMDLGCYRGLRHRRGLPTRGQR
                     THTNARTRKGPRKGMISRSRAKKS"
     gene            complement(3042..3161)
                     /gene="rpmJ"
                     /locus_tag="PK710_07435"
     CDS             complement(3042..3161)
                     /gene="rpmJ"
                     /locus_tag="PK710_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOT09584.1"
                     /translation="MKVRPSVKKICDKCKIVKRNGVVRVICKKNPRHKQRQGA"
     gene            complement(3172..3426)
                     /gene="infA"
                     /locus_tag="PK710_07440"
     CDS             complement(3172..3426)
                     /gene="infA"
                     /locus_tag="PK710_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOT09585.1"
                     /translation="MRPGGNRKDRTTQYEEGKLEARGLVTQVLPNAMYRVCCESQREV
                     LATVSTKLRQHMIKILPGDQVTVELSPYDPNRGRITCRHK"
     gene            complement(3430..4761)
                     /gene="secY"
                     /locus_tag="PK710_07445"
     CDS             complement(3430..4761)
                     /gene="secY"
                     /locus_tag="PK710_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOT09586.1"
                     /translation="MAFANFANIPKVPELRKRLLFTLFMLAIYRVGVFITAPGIDRNV
                     MRSVVQRQSGGLLGMFNLFTGGALENLSIFALGIMPYISSSIIMQLLGTVYKPIDDMR
                     REGEQGRRKLDQYTRYGTVVLSIFQSFGIAMYLEGLNHHEMGGSGGMGDVVANPGWAF
                     RLMTIITLTTGTAFIMWVGEQITERGVSNGISLIIFAGILSDLPSVAVGYVQQHEGNL
                     QPLTIASMLALIVGTVAVVVFFERAQRRIPIVYSRRQVGRRVYGGQTAHLPLKVNSAS
                     MIPAIFASSLLMFPATLANLNIPGMNNIQAVLNKGGWVFNTFYALLIIFFCFFYTAVT
                     VRPVDIAENLKKQQANVPGIRPGKQTAEYIDRVLTRITLGGSLYVAAVCLIPNIASAW
                     YNVSFRFGGTSIMIVVGVALDLSSSIESHLITHNYEGLTGPKVSRIRGRQV"
     gene            complement(4774..5265)
                     /gene="rplO"
                     /locus_tag="PK710_07450"
     CDS             complement(4774..5265)
                     /gene="rplO"
                     /locus_tag="PK710_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOT09587.1"
                     /translation="MADILSRLQAPKGANRPKTRVGRGVGSGLGKTCGRGQKGQKARN
                     TGRFSKRAFEGGQTTLQRRLPKRGFVNVFRAQVAIVNVGDLERFESGTTVDEDLLRRS
                     RLVRGKFDLIKVLGQGDLGKALTVTVHTFSKSAIEKIEKAGGKVIPLIPLETPEAGEA
                     SAE"
     gene            complement(5268..5456)
                     /gene="rpmD"
                     /locus_tag="PK710_07455"
     CDS             complement(5268..5456)
                     /gene="rpmD"
                     /locus_tag="PK710_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOT09588.1"
                     /translation="MKPKLKVRQVRSLSNRPYPQHQVIAGLGLRGVGSEVVVDNTPSF
                     RGMVKKVLHLVQIEEVEG"
     gene            complement(5453..5998)
                     /gene="rpsE"
                     /locus_tag="PK710_07460"
     CDS             complement(5453..5998)
                     /gene="rpsE"
                     /locus_tag="PK710_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003133760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOT09589.1"
                     /translation="MQFDQTLQLDEEKLKERVIHINRVAKVVKGGRRFSFSALVVVGD
                     ESGHVGVGLGKANEVPEAIRKGNDQARKSMFRVPLVGGSIPHGIIGRFGAGRVMLRPA
                     SEGTGVIAGGVVRAVVESAGIKDILTKCMGTTNPHNVVRATIEALRTLACVESVAAKR
                     DKSVEDVKPHTQDATQQGVAP"
     gene            complement(6012..6383)
                     /gene="rplR"
                     /locus_tag="PK710_07465"
     CDS             complement(6012..6383)
                     /gene="rplR"
                     /locus_tag="PK710_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOT09590.1"
                     /translation="MAMRLVGRDRRKVRIRKKVTGTSERPRLTVFRTARHIYAQVIDD
                     VKGCTLAHASTLSVDVRDANAQEEKKLKKTEKATQVGALIAKICLSKDIKQVVFDRNG
                     FKYHGRVSALAAAARKAGLKF"
     gene            complement(6413..7003)
                     /gene="rplF"
                     /locus_tag="PK710_07470"
     CDS             complement(6413..7003)
                     /gene="rplF"
                     /locus_tag="PK710_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOT09591.1"
                     /translation="MTQVAEKEVRTSRIGKLPVTIPKGVTVSVANGSVTVKGTKGTLT
                     WSPPKGTAVAVEGEKVHVTAGTGVGSARMQGLTRALIANMVRGTSEGFSKTLLLVGTG
                     YRAEVKGPELHLSVGLSHPVVFPIPEGISVTVPKDSKGAQIVITGADRGLVGETAARI
                     RSTRPPEPYGGKGVRYQGEQVREKAGKAGKGAKGAR"
     gene            complement(7003..7404)
                     /gene="rpsH"
                     /locus_tag="PK710_07475"
     CDS             complement(7003..7404)
                     /gene="rpsH"
                     /locus_tag="PK710_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOT09592.1"
                     /translation="MMTDPIADMLSRIRNAALARHDRVSMPISKVKRAVADLLKQEGY
                     VRDVTISESDGKKSPTLTVVLKYGQDRTCAIDGLKRVSTPGRRVYVRHDSIPLVQNGL
                     GVGILSTSRGVMTDRDARRQGIGGELLCEVW"
     gene            complement(7409..7594)
                     /locus_tag="PK710_07480"
     CDS             complement(7409..7594)
                     /locus_tag="PK710_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOT09593.1"
                     /translation="MARACSFAKLNRKPKFAVRHRNRCKMCGRARGYYRDFELCRICM
                     RNLALRGELPGVVKASW"
     gene            complement(7606..8151)
                     /gene="rplE"
                     /locus_tag="PK710_07485"
     CDS             complement(7606..8151)
                     /gene="rplE"
                     /locus_tag="PK710_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOT09594.1"
                     /translation="MVPPRLRDKYREQVVPAMTKKFAYANPMQVPRIEKVVVNMGLGQ
                     AVGNPKVIEAAVDELSDITGQKPVVARAKKAISNYKLRTGMPIGVFVTLRREHMWEFL
                     DRLITVALPRVRDFKGVSAKGFDGRGNFTLGLREQIIFPEINYDKVDSTRGMNVSIVT
                     SAKTDEEGRALLAELGMPFRS"
     gene            complement(8322..8645)
                     /gene="rplX"
                     /locus_tag="PK710_07490"
     CDS             complement(8322..8645)
                     /gene="rplX"
                     /locus_tag="PK710_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOT09595.1"
                     /translation="MQRIRRGDMVQVIAGKYKGAKGRILAVLRDKDMVLVENVNVKKR
                     HVKPNARMPQGGIIEREAPIHACKVMPLDPETGLPTRVRIQTNEEGKKERVAVKSGAV
                     IVANS"
     gene            complement(8645..9013)
                     /gene="rplN"
                     /locus_tag="PK710_07495"
     CDS             complement(8645..9013)
                     /gene="rplN"
                     /locus_tag="PK710_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOT09596.1"
                     /translation="MIQMRTVLDVADNSGAKRVQCIKVLGGSRRKYAGIGDVIVVSIK
                     EAMPNMKVKKGDIAKAVIVRTAQSIARPDGSFIKFDGNSAVLVNQQMEPIGTRIFGPV
                     ARELRSKKFMKIVSLAPEVL"
     gene            complement(9022..9342)
                     /gene="rpsQ"
                     /locus_tag="PK710_07500"
     CDS             complement(9022..9342)
                     /gene="rpsQ"
                     /locus_tag="PK710_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOT09597.1"
                     /translation="MSDDTKDTAQAEQNEAAPVQAATGFKRKLAGRVKSAKMAKTVVV
                     QVVRYFRDPVYKKYVRTRTSYKAHDEENQCRVGDRVEIIESRPLSREKRWKVVRVLER
                     AEID"
     gene            complement(9346..9555)
                     /gene="rpmC"
                     /locus_tag="PK710_07505"
     CDS             complement(9346..9555)
                     /gene="rpmC"
                     /locus_tag="PK710_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOT09598.1"
                     /translation="MKAKDLRDRGTDDLRELDKRLTHDLFQYRFKNHTGGLDDTSLVR
                     KTRRDIARVRTELAARERAAKDEAR"
     gene            complement(9552..9968)
                     /gene="rplP"
                     /locus_tag="PK710_07510"
     CDS             complement(9552..9968)
                     /gene="rplP"
                     /locus_tag="PK710_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOT09599.1"
                     /translation="MLSPKRTKYRKQQKGRRRGLAYRGSDISFGDFGLQVLEPGRLTA
                     RQIEAARMAITRHVKREGTLWIRVFPDRPVTKKPLEVRMGGGKGAPDSWAAEVKPGRV
                     MYEISGVTEEKAREAFRLAAHKLPLACKFIARGELS"
     gene            complement(9982..10674)
                     /gene="rpsC"
                     /locus_tag="PK710_07515"
     CDS             complement(9982..10674)
                     /gene="rpsC"
                     /locus_tag="PK710_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOT09600.1"
                     /translation="MGQKTHPYGFRLGVIKTWTSKWYEDKSYTKWLHEDIRIKRAVKS
                     YLYNANIAGVEIERAANKAKVIVYTARPGMVIGKGGEKIETLKVGNLKTAKKDEPKFA
                     GVQAFTDNEVFIDVQEVRKAETNAQLVAENIATQLERRVAFRRAMKKSVATAMKFGAK
                     GIRVRCAGRLGGSEMSRVETYREGRVPLHTLRADIEFGTAEARTTYGIIGVKVWIFKG
                     EVLARKPRRIPA"
     gene            complement(10690..11031)
                     /gene="rplV"
                     /locus_tag="PK710_07520"
     CDS             complement(10690..11031)
                     /gene="rplV"
                     /locus_tag="PK710_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOT09601.1"
                     /translation="MISKANAMYARIAPRKARVVANLVRGKDVGAALMTLEFLRKSAA
                     PLIRKVIESALANARTGKSDANVDDLYVSKITVDKGPNRQMRRWRPRAMGRATRIVKG
                     VSHIMVELDER"
     gene            complement(11258..11923)
                     /locus_tag="PK710_07525"
     CDS             complement(11258..11923)
                     /locus_tag="PK710_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOT09602.1"
                     /translation="MTTTIAHRLDQVRQRIEQAARRGGRRAQDVLLIAVSKTQPESAI
                     RAAYDAGQRDFGENYVQELVQKAHALRDLPDLRWHLIGHLQRNKVKTVLDVVQVVHTV
                     DRLALVTELGKRLSAPLDVLVEINVGQESSKTGASPDQTPLLVEAVRAQAGLRLQGLM
                     TVPPFDLDLPTVGEYFDRLRGLAQAFGLKELSMGMSHDFEEAIVRGATMIRVGTAIFG
                     DRG"
     gene            12135..12509
                     /locus_tag="PK710_07530"
     CDS             12135..12509
                     /locus_tag="PK710_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09603.1"
                     /translation="MLSGSIYTCAINPQPEPPGSNATATPEGGGGGSGETDIDAAPHF
                     GGSGGASIGGTGGGAGSGGQTLSDAGPDSDEDAPTLDPDAWDDADADTDSDAEDDADA
                     EDDADDAQVDTANDSPLDSAID"
     gene            12561..>12743
                     /locus_tag="PK710_07535"
     CDS             12561..>12743
                     /locus_tag="PK710_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09604.1"
                     /translation="MASLRRWCVTVLLCSSAWVAACTINPQPEPPGSQDTADRNEAGT
                     GGQFGGVADAGISDCKT"
BASE COUNT         2431 a         3856 c         3497 g         2959 t
ORIGIN      
        1 tcgccgcggc gggggccgag tttgatgatg cgggtgtacc cccccggtcg tccttggaat
       61 cgcttggcga ggtcgatgag gactttttcg accaggtcga tgcgttgcat cgccccttct
      121 tcgttggcga ccattccgta gcgagggatg aaggcgcgga tttgtctcgc cacgtgaaga
      181 cgccgtgcga tctggcgtcg atcgggggtt tcccctggtt tgttgtaggc gacatcccca
      241 aggcgaatgg ctttggtgat gagtctttcg gcgacgcggc gcagttcttt ggctttggcg
      301 tcggtggttt cgatgcgttc gtgagtgatg aggttggccg tgaggtttcg caacatcgcc
      361 cgacggctgc tggggtttcg gccgaacttc cggcctgctt tcaagtgacg catggcgagc
      421 tgtcctcttg tttagttctg gctctgctgc gccttatagc gctccaggag ttgtggccag
      481 ttgtcaatct tcattccgag ggaaaggccc atgctcgcga ggatttcctt gatttctttg
      541 agcgattttc gcccgaagtt tttggtcttg agcatgtcct ggtcggtgcg ctgaaccagc
      601 tcaccgatga gtgtaatatt cgcgttctgc atgcagtttg cggaacgaac gctgagttcg
      661 agttcatcga cggatcggaa caagttctcg ttgataggct cttcgctatc gcttggcgca
      721 acgtacgagg tttcttcggt ttcctcgaag ttgatgaaga tggtgagctg ttctttgagg
      781 atttttgcag cgaacgccac cgcatcttcg ggtcgcacgg caccattggt ccacacttcg
      841 agcaccaatt tatcgtagtc cgtgacctga ccgacgcgcg cgttctgcac cgtgtagttg
      901 accttgcgca ctggagaaaa gagggcatcg atcggaatgg tgcccagcgg catggtggcc
      961 gttttgttgc gttcggccgg cacatatccc cggcccattc cgacggtaag ctccatcgaa
     1021 agcgggcctt ttttatccaa tacggcgatg acgtggtcgg ggttgagcac ctcgagtcca
     1081 tcgaccaaat ggatatcctt ggcgcaaacg gggcctggtc cttctttatc gattcgaacc
     1141 acgaagttgt cgagtcgatg ggctttgaac acgacctctt tgatgttgag gatgatgtcg
     1201 ctcacgtctt cgacgacatc tcggatggtg gtgaactcat gcaatgcgcc atcgatccgg
     1261 accgccgtaa cagcagcgcc ttgcagcgac gatagaagca cgcggcgcag ggagtttccg
     1321 acggtgatac cgaaccctcg ctcgaggggt tcgcaggtaa acttggcgta cacatcggag
     1381 cgggattccg cgtcgatctg gatagctttg ggacgaatga gttcccgcca attgcgcgca
     1441 atgagttgtt ccatccgtgc atctcctcgt ttgaaggttc gcgcgggaat cccagccatc
     1501 ttgccgaacc cgctcgttca aggcggctcg ttccctgccc gcgtttgggt atctatccta
     1561 ccgtgagtaa tactcgacga tgagttgttc gcggatagcg ggctcgttga gttcctcccg
     1621 cagggggctc gacttgatga ccgccgaata cgagccgcga tccaggtcca tccagccagg
     1681 gacggggcga gcctccgcca tggcgatgga ggattcgaga agcttgagtt tacggcttcg
     1741 ctcgcgcacc tcgactttgt ctccgatacg caagatatag ctcgggatgt tgactcgccg
     1801 tccgttgacc aagatgtggt tatgcttcac gagctgtcga gcttctcttc gggtaacgcc
     1861 gaatcccatg cgatacacgg cggagtcgag tcgtcgctcg agcaaagcga gcatttgctc
     1921 accggtgccg ccacgcatac ggttggcgcg gtcgtaatag aggcggaact gtcgttcctg
     1981 cactccatat acgcgacgca ccttttgctt ttcgcgcaaa cggatggcgt actcgctcaa
     2041 cttgatgcgc ccttggccgt gctgcccagg tgggtaagaa cgacgagtga aagcgcattt
     2101 ttcggtctgg cagcgctctc ccttgaggaa gagtttttcg ccttcacgac ggcaaagttt
     2161 gcaaacgggg ccgatatatc gagccatgaa taagtcctca gccgatcaca cgcgacgccg
     2221 cttgggcggg cggcatccgt tgtggggaat cggggttaca tcgcgaatca aagtgacttt
     2281 gaaaccggcg gactgcagag cgcgaagagc gctctcccgg ccagcacccg gtcctttgac
     2341 gaacacggcg acagaacgca caccatgctc catggcgcgt cgagcagctt cctctgcagc
     2401 gagctgcgcg gcgaacggag ttgacttgcg ggagcctttg aatccgcggg atcccgcgct
     2461 ggaccaggac accacgttgc cgttgacgtc gctgatcgtt acgattgtgt tattgaacgt
     2521 cgactggata tgggcaatcc cagaaccgat gttcttcttg acctttttct tctgtttgac
     2581 gcccttgccg cgggctttgg tcgtactcat cgcgcggtac tccgtctaac tcttcttcgc
     2641 ccgggagcga gagatcatgc ccttacgcgg tcccttacgg gtgcgggcgt tggtgtgggt
     2701 acgctgccca cgcgtgggta agccacgacg atgtcgtagc ccgcggtagc atcccaagtc
     2761 catcaatcgc ttgatgttca tttggacttc gcgacgcaga tcgccttcga ccttgtactc
     2821 gccatcgatg atctcgcgaa tcgccttgat ctcggattcc gcgaggtcct cgacacgctt
     2881 ggacggagaa acctgcgctt tttcgcagat atcgcgagcc agctttcgtc caattccgta
     2941 aatatacggc agcgcataca caacgtgttt gcgccgcgga aggtcaacac ctgcaatacg
     3001 tgccatagaa tccctactcc tgctcgatca tcggggtcgg ctcaggcgcc ttggcgctgc
     3061 ttgtggcgag gattcttttt gcagatcacg cgcaccacgc cgttgcgctt gacgatcttg
     3121 cacttgtcgc aaatcttctt cacggatggc cgaaccttca taactgcctc tctacttgtg
     3181 ccggcatgtg atccggcctc gattgggatc gtacggcgat aattcgacgg tcacctggtc
     3241 acctggaagg atcttgatca tgtgctgacg caatttcgta ctgaccgttg ctagcacttc
     3301 ccgttggctt tcacagcaga cccgatacat cgcgttgggt agcacctgcg tcaccaaccc
     3361 tcgtgcctca agcttacctt cctcgtattg tgtcgtccga tccttccgat ttcctcctgg
     3421 tcgcatgcct caaacctggc gtccacgaat gcgtgacacc ttgggtcccg tcaatccctc
     3481 ataattgtga gtgatgaggt gggattcgat gctcgaagat aagtcgagcg ccacccctac
     3541 cacaatcatg atggacgtgc cgccaaatcg gaacgacacg ttataccatg cgcttgcgat
     3601 gttcgggatc aagcacaccg cagccacata caatgaacca ccaagtgtga tccgggtcag
     3661 cacccggtca atatattcgg cggtttgttt gccagggcga atccctggaa cgttggcttg
     3721 ctgcttttta agattctccg caatatctac cggacgaacc gtcacggccg tatagaaaaa
     3781 gcagaagaag atgatcagca acgcgtaaaa cgtgttgaac acccatccac ccttgttgag
     3841 caccgcctgg atattgttca tccccgggat attcagattc gcgagcgttg ccgggaacat
     3901 cagcaacgaa gaagcgaaaa tcgcgggaat catgcttgcc gaattcacct tcaacggcag
     3961 atgcgccgtc tgacccccat aaaccctacg gcctacttga cgtctcgagt aaacgatcgg
     4021 gatccttctc tgagcccgct caaaaaacac gacaaccgcc acggtgccga cgatcaacgc
     4081 gagcatcgac gcgatggtca ggggctgcag gttaccttcg tgttgttgca cgtaaccgac
     4141 ggcaaccgac ggcaaatccg acaggatacc ggcaaaaatg atcagcgaaa tcccgttgga
     4201 aacgcctcgt tccgtaattt gctcgcctac ccacatgatg aacgccgtgc ccgtggtaag
     4261 cgtgatgatc gtcatcaatc gaaatgccca gcctgggttg gcgacgacat ctcccatccc
     4321 gccgctccct cccatttcgt gatggttcag gccctcgaga tacattgcga tcccaaagga
     4381 ttggaagatg ctgagcacca cggttccgta ccgtgtgtat tgatcgagtt tacggcgacc
     4441 ttgttcgccc tctcgtcgca tatcatcgat cggcttatac acggtgccga gcagttgcat
     4501 gatgatcgac gacgagatgt agggcatgat cccgagcgcg aagatcgaga ggttttcgag
     4561 cgccccgccg gtaaagaggt tgaacatccc cagcaatccg cccgattgac gctgaacgac
     4621 cgaccgcatg acatttcgat cgatgcccgg agcggtaatg aaaactccca cgcgataaat
     4681 cgcgagcata aagagggtga acagcaacct ctttcggagt tctggcacct tcggtatgtt
     4741 cgcgaagttg gcgaatgcca tgattctcgt cgcctattcg gcagacgcct ccccggcttc
     4801 cggcgtttcc aaaggaatca agggaatgac cttgcctccc gccttttcga tcttctcgat
     4861 ggcggacttc gagaacgtat gcacggtgac ggtcaatgcc ttgccaaggt cgccctgtcc
     4921 aagcaccttg atcaaatcga acttgccgcg aaccaatcgc gatcgtcgaa gcaaatcctc
     4981 gtctaccgtc gtccccgact cgaaacgctc gaggtcgccc acgttgacga tcgccacctg
     5041 agcgcgaaaa acgttgacaa atccacgctt tggcaatcgt cgctggagcg tggtttgccc
     5101 accttcaaac gctcttttgc tgaaccggcc agtatttctg gctttttgac ccttttggcc
     5161 ccggccgcag gtttttccaa gtcccgaacc cacaccgcgt ccaacgcgag tcttaggtcg
     5221 gttggcccct ttcggggcct ggagtctaga aagaatgtcc gccatggcta tccctcgacc
     5281 tcttcgatct gcacgaggtg caagaccttc ttgaccatgc cgcggaacga cggtgtgttg
     5341 tccacgacga cttcagagcc cactccacgg agccccaatc cagcgatcac ctgatgctgt
     5401 ggataaggtc gattggagag gctgcggacc tgacggacct tgagcttggg tttcacggcg
     5461 cgaccccttg ctgcgttgca tcttgcgtgt ggggtttgac gtcctccaca ctcttatcac
     5521 gtttggcggc aaccgattcg acacacgcca gggtacggag agcctcgatg gttgcgcgaa
     5581 cgacgttatg cgggttggtg gtccccatgc acttggtgag gatatccttg attccagcgg
     5641 attcgacgac ggcacggacc acaccaccgg cgataacgcc agtaccttcc gatgcggggc
     5701 ggagcatcac gcgcccagct ccgaaacgac cgatgattcc gtgggggatc gaccctccca
     5761 cgaggggcac ccgaaacatg cttttgcgtg cctggtcgtt accttttcgg atggcttccg
     5821 gcacctcatt ggccttacca agccctacgc cgacgtgacc tgattcatca ccgaccacga
     5881 cgagggcgga gaaactgaag cgacgaccac ctttcaccac cttggcaaca cggttgatgt
     5941 gaatgacgcg ctccttgagc ttttcttcgt cgagctgaag tgtctgatcg aattgcatga
     6001 ttcctcgcta cctagaactt cagcccggct tttcgcgccg cggcagccag agcgcttaca
     6061 cgcccgtgat acttgaaacc atttcgatcg aaaaccacct gcttgatgtc tttcgacaag
     6121 cagatcttcg cgatcaaggc acccacttgg gtggccttct cggtcttctt gagcttcttt
     6181 tcttcctgtg cattcgcgtc ccgcacatcg accgacaacg tggaagcatg cgccaaggtg
     6241 cagcccttca cgtcatcgat gacctgcgca taaatatgcc gcgcagtacg aaatacggtc
     6301 aatcgcgggc gttcggaagt gcccgtgacc tttttacgaa tgcgaacctt gcgcctgtcg
     6361 cgtccgacca gtctcatagc catcgtcaag actccgcgct gggatcagcc atttacctgg
     6421 cgcccttcgc gcccttgccg gccttgccgg ctttctcacg aacttgctcg ccctgatagc
     6481 gaacgccctt gccaccatac ggttccggcg gccttgtgct gcgaatccga gctgccgtct
     6541 caccgaccaa acctcggtcg gcgcccgtga tgacgatctg cgctcccttc gagtctttcg
     6601 gcaccgtcac cgagattcct tcagggatgg ggaataccac cggatgggac aaccccactg
     6661 ataagtgaag ttcgggtccc ttgacctctg cccggtatcc ggttcccacc agcagcaagg
     6721 ttttggaaaa cccttccgag gttccgcgaa ccatgttcgc gatgagagcc cgggtcaacc
     6781 cttgcatgcg tgcggaaccg acaccggtac cggcggttac atgcactttt tccccttcca
     6841 cggcaacagc cgtacccttg ggggggctcc aggtcaaggt gcctttcgtt cctttgacgg
     6901 tcaccgatcc gtttgcgacg gacactgtaa cccccttcgg aatcgtcacc ggcagctttc
     6961 cgatccgcga ggtgcggacc tctttttcgg ctacttgcgt cattaccaca cctcgcatag
     7021 cagctcaccg ccaatgcctt gacgacgcgc gtctcgatcg gtcatgacac cccgtgacgt
     7081 ggataggatg cccacgccca atccgttctg aacgagcgga atgctatcgt ggcgcacgta
     7141 cacacggcgt ccgggggtgg agacgcgctt gaggccgtcg atcgcgcagg ttcgatcttg
     7201 gccgtatttg agcacgacgg ttagggttgg ggattttttt ccatcggatt cggagatcgt
     7261 cacgtcgcgg acgtagcctt cttgcttgag caagtcagcg acagcgcgct tgactttgct
     7321 tatgggcatc gagactcggt cgtgacgcgc gagggctgcg tttcgaatcc gactcagcat
     7381 atcggcgata ggatcagtca tcatggcatc accaacttgc cttgactaca ccgggaagtt
     7441 ctccacgcaa cgccaggtta cgcatgcaga ttcggcatag ctcgaagtcg cggtagtagc
     7501 cgcgtgcacg gccgcacatc ttgcatcggt ttcgatgcct gactgcgaac ttcggcttcc
     7561 ggttcaactt ggcaaaagag caagctcgcg ccatcgttca tcccttcagg aacggaacgg
     7621 catgcctagc tcagcgagca gcgcacgccc ttcttcatca gtcttcgccg aggtcacgat
     7681 cgacacattc atcccgcggg tgctgtcgac cttgtcataa ttgatctctg ggaaaatgat
     7741 ctgctcgcgc agtcccagcg tgaagttgcc ccgtccgtcg aaccccttgg cgcttacgcc
     7801 tttgaagtcg cggacccgcg gcaacgccac ggtaatcaat cgatccagaa actcccacat
     7861 gtgctcgcgt cgcagcgtca caaacacgcc gatcggcatt cctgtgcgga gtttgtaatt
     7921 cgaaatggcc tttttcgccc gagcgacgac cggtttttgg ccggtgatat cactcaactc
     7981 gtccacagcc gcttcaatca ctttgggatt tccgacggct tgcccgagcc ccatgttgac
     8041 cacgaccttt tcgattcgtg gaacttgcat cgggttggca taggcgaact ttttggtcat
     8101 cgcagggacc acttgctcgc ggtacttatc tcgcaggcga gggggaacca cagggcgcga
     8161 aggcacgggg gcagcgtcgc ccttggcctt tttgtccttg cttttcgctt cctgcttgcc
     8221 cttggcctcc tgcttaccct tggcctcagg cttgcctttg ccacccttat ccgcggcttt
     8281 ggctttgctc tctttctctt cgctcttcgc catgacaagc ctcaggaatt cgccacgatg
     8341 accgcgccgc ttttcacagc gacacgctct ttcttacctt cttcattcgt ctggatacga
     8401 acccgtgtgg gtagacccgt ctcagggtcc aagggcatca ccttacaagc gtggatcggc
     8461 gcctcgcgtt cgatgattcc accctggggc atacgtgcat tcggtttgac gtgacgcttt
     8521 ttgacgttca cgttctcgac cagcaccata tccttatcgc gcagcacggc gaggattcga
     8581 cctttcgcac ccttgtattt gccggcgatg acctgcacca tgtcgccacg tcgaatgcgc
     8641 tgcatcacaa cacctccggg gcaagcgaca cgatcttcat gaactttttg gagcgaagct
     8701 cgcgagccac cggcccgaaa atccgcgttc cgatcggctc catctgctgg tttacaagga
     8761 ccgcgctatt gccatcgaac ttgatgaagc tcccatcggg gcgcgcgatg ctctgcgcgg
     8821 tccgaacgat cacggctttc gcgatatcgc ccttcttgac cttcatgttc ggcatcgctt
     8881 ccttgatgga cacaacgatg acgtctccaa tcccagcgta ctttcggcgg gacccaccaa
     8941 gtaccttgat gcactgcact cgtttcgcac ccgagttatc ggcaacatca agaaccgtgc
     9001 gcatctggat catggctcac ctcaatcgat ctcggcacgc tcgagaacgc gcacgacctt
     9061 ccaccgcttc tcccgtgaca gcgggcggct ttcgatgatt tcgactcggt cgcctacgcg
     9121 acactggttt tcttcatcgt gggctttgta gctcgtccgc gttcgaacgt attttttata
     9181 cacggggtct cggaagtagc gcacgacctg caccacgacc gttttcgcca ttttggcgct
     9241 tttcacgcgc ccagcgagct ttcgcttgaa gcccgtggcc gcttggacgg gcgcggcttc
     9301 gttctgctcg gcttgcgcgg tgtctttggt atcgtcggac atggcttacc tcgcctcgtc
     9361 tttcgctgca cgctcccgcg ccgcgagttc cgtgcgcaca cgagcgatgt ctcgtcgcgt
     9421 tttccgaacc agactggtgt catcaagacc gccagtgtgg tttttgaagc gatattggaa
     9481 cagatcgtgg gtgagccttt tgtcaagctc ccgcagatcg tccgttcccc ggtcacgcaa
     9541 gtctttcgct ttcatgacag ttctcctcgg gcgatgaact tgcaggccaa gggcagttta
     9601 tgggccgcaa gacggaacgc ctcgcgggcc ttttcttccg taacgccgga gatttcgtac
     9661 atgacgcgac cgggcttgac ttcggcggcc caggaatccg gggcgccttt tccgccgccc
     9721 atccggactt ccaagggttt tttggtgacg ggacggtctg ggaacacgcg gatccacaga
     9781 gtgccttcac gtttgacatg ccgtgtgatc gccatacgtg cggcctcgat ttggcgcgcg
     9841 gtcaatcggc ccggttcgag cacttgcagc ccgaagtcac caaaggagat gtcgctgccg
     9901 cgataggcta agccgcgacg gcgtcccttt tgctgttttc ggtacttcgt tcgcttggga
     9961 gaaagcatct tcctgctcca actacgcggg gatccgccgc ggttttcgcg ccaaaacctc
    10021 gcccttgaag atccaaacct tcacaccgat gatgccgtac gtcgttcggg cttccgccgt
    10081 accaaactcg atgtcggcgc gtagcgtatg caagggaacg cgtccctcac gatacgtctc
    10141 gacccggctc atttcgctgc cacccagacg tccggcgcac cgaacgcgga tgcctttggc
    10201 tccgaatttc atggcggtgg caacggattt tttcatggct cgacgaaacg cgactcgtcg
    10261 ctcgagctgg gtggcaatgt tctcagcgac gagctgggcg ttggtttccg ccttgcgcac
    10321 ttcttggacg tcgatgaaga cctcattgtc ggtgaaggct tgcacgccgg cgaatttggg
    10381 ttcgtccttt ttcgcggttt tcaagttccc gactttgagc gtttcaattt tctcgccgcc
    10441 cttgccaatc accattccgg gcctcgcggt gtacacgatg accttggctt tgttggcggc
    10501 tcgctcgatc tccacgccag cgatgttcgc gttatataag tacgacttaa ccgctcgctt
    10561 gatgcgaatg tcttcgtgaa gccatttcgt gtaggacttg tcctcgtacc atttcgaggt
    10621 ccacgtcttg atgacgccaa gtcggaagcc gtatgggtgc gtcttctgtc ccatgaatac
    10681 cctcgtatcc tagcgctcgt cgagttcaac catgatgtga ctcacacctt tgacgatgcg
    10741 agtcgctcgg cccatcgcac gaggccgcca acggcgcatc tggcgattcg gtcccttgtc
    10801 caccgtgatt ttgctcacgt aaaggtcgtc aacgttcgcg tcgctttttc ccgtgcgagc
    10861 gttggcaagg gcgctttcaa tgacctttct gatgaggggc gcagccgatt tacgcaaaaa
    10921 ctcgagcgtc atcaacgcgg cgccgacgtc ctttccccgg accaaattgg cgaccactcg
    10981 ggccttgcgc ggcgcaatcc gcgcgtacat cgcattcgcc ttactaatca tcggacttcc
    11041 tctgccgacc gtcgtacgca cggggaaacg gatctgaagc tggtcttcga ccggcttttg
    11101 tttggcgatc cgcttcgcgc gccgttacgg ctggcttgtc aactgccccg ccgaggggac
    11161 tcggggttcg gggaggcgcg tagtagcaag ctagttggaa gcatgcaagt agttttttgg
    11221 tcgacggcgt tttttctgaa aaccttcgaa accgctgtca accacgatcc ccaaagatcg
    11281 ctgttcccac acggatcatc gtcgcaccac gaacgatcgc ttcctcgaag tcatgggaca
    11341 tgcccatcga cagttccttc aacccaaagg cttgcgccag accgcgtaat ctatcgaagt
    11401 actcacctac cgtcggcaga tccaaatcga aaggcgggac ggtcatcaag ccttgcaagc
    11461 gcagcccggc ttgggcacgc accgcctcca ccaacagcgg agtctggtcg ggactcgcgc
    11521 ctgttttgct tgattcttga cctacgttga tttccaccaa aacgtcgagg ggagccgata
    11581 accgttttcc caactcggtc accaacgcga gtcgatcgac cgtgtgaacg acctgcacca
    11641 catccaagac tgtcttgact ttgtttcgtt gcaggtggcc gatgaggtgc caccgcagat
    11701 ccggaagatc ccgcaaggcg tgcgcttttt gcaccagttc ttgaacgtaa ttctccccaa
    11761 aatctcgctg ccccgcgtca taagccgcgc gaatggccga ctcgggttga gtcttcgaaa
    11821 ccgcgataag tagcacgtct tgcgcacgac gccccccccg tcgtgcagcc tgctcgattc
    11881 gctgacgtac ctgatcgagc cgatgggcaa tggtggttgt catgagttac gaagtcctgt
    11941 ttggacgcct ttcatagcat gaagggagca gaccgctcac gaccgacaac tggatcgggc
    12001 atgtgtcact atgtgccgca gcgtgtcact cgggccaaac cgaaattggc gtatttctcg
    12061 tgtgtcatca tggcgcaagt catgctttaa cctatcttat gcgcgctcgg ttctggccct
    12121 acctctttgt ctttatgcta agtggcagca tctacacatg cgccatcaac ccgcaaccgg
    12181 aaccgccagg cagcaacgcc accgcaactc cggagggcgg cggcggaggc agcggcgaga
    12241 ctgatatcga tgccgcgccg cacttcggag gcagtggcgg cgcgtccata ggagggaccg
    12301 gcggtggcgc gggatccggc ggccaaacgt tgtccgatgc cggaccggac agcgatgaag
    12361 atgccccaac gctcgatcca gacgcgtggg acgacgcgga tgccgacacc gactcggacg
    12421 ccgaggacga cgccgatgcc gaggacgatg ccgatgacgc acaggtagac actgcaaacg
    12481 acagtcccct ggatagcgca atcgactgac gatctggccg cccgagccag atggaccagt
    12541 tcctgctatg ccttatcacc atggcgagtt tgcggcgttg gtgtgtcacg gtccttctgt
    12601 gttcaagcgc atgggtcgct gcttgcacca tcaatcccca acccgaaccg ccaggcagcc
    12661 aagacaccgc agaccgaaat gaggctggca cgggaggtca attcggagga gtggccgacg
    12721 cgggcatcag cgactgcaaa acc
//
LOCUS       DAOSOR010000099        12678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4953_length_12678_cov_4.440624, whole genome shotgun sequence.
ACCESSION   DAOSOR010000099 DAOSOR010000000
VERSION     DAOSOR010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 12678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12678
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4953_length_12678_cov_4.440624"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PK710_07540"
     CDS             <1..377
                     /locus_tag="PK710_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOT09605.1"
                     /translation="VKPVATLEQLSQARGVVRDIYADERVKQYIVDVVRATREPERYG
                     IKDLAGLIHYGASPRASIALAFAGKAHAFLRRRGHVAPEDIKAIGPDVLRHRLVLTYE
                     AEAEEVSTEDVVRRVFEAVEVP"
     gene            390..1274
                     /locus_tag="PK710_07545"
     CDS             390..1274
                     /locus_tag="PK710_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOT09606.1"
                     /translation="MIPKELLKQLRKIEFVTAKLANEELSGSYTSVFKGQGLAFRDVR
                     QYQRGDDVRKIDWNVTARMNEPFVKVFVEERELTVMLVVDASGSSLFGTVRAPKSQVA
                     GEVAALAAFSAIHNNDRVGLVLASDHLEKLVPPKKGEKHVLRVVREILAFQPQSHGTN
                     LAIAFETLVKVARRRSLAFVISDFFDDGFERAMALASAKHDIIPVMLVDPRDESLPDV
                     GLATFEDLESGESITVDTSDARVREHYAQSMKAVRDERIRVFRKHKLDLCVVRTDGSF
                     VAPLRDLFAHRAKRVRSR"
     gene            1271..2485
                     /locus_tag="PK710_07550"
     CDS             1271..2485
                     /locus_tag="PK710_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09607.1"
                     /translation="MKRGKAFGLLLGLGLTLGGQASAQDASSEAAVDGSVSDADVARQ
                     PRADAGPSEAGTDALPIGEDAGRIWASCVEHVPEGATRPKLTNTFPTRGLAGHALTLE
                     IQLEHGLGETVLPNGFRIQADSDAARALSSAGFELPNPNGGSRPTLTRHEERDKATTN
                     VRVHFLSLPAKPGRHELTLPPIPIAVSRASGEVLTLCTTPHTIIVDEPIANEPEAAPL
                     GNPEPRPQREVWTLARDLTYGGLIGVVVAALALWFILWWQRRPKPPIPPPPPRPPWEV
                     ALESLHRLRGLQPHDDAERGEHVDQVSDIVRIYLGARFGFDGIESTTSETLRALEKIQ
                     PPLLVFDDIRQLLFDSDLVKFARWSPTNEQCDQLLDMAEKIVKATMPASMPPSSAATS
                     EDSKSSKQEGET"
     gene            2482..3675
                     /locus_tag="PK710_07555"
     CDS             2482..3675
                     /locus_tag="PK710_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT09608.1"
                     /translation="MKKTSISPSSTPKKQQGRRTAIRVLILLALLIAVMALAFAYPLA
                     MRGWNWLHVTWRHPWALLLLLLAPIVLWRGTLGEDNRLPRMRMGTLHPFLQGPQGWRA
                     RLKDLPGIFRAAAITMLVMALAQPISMMRPESTDELGIDIVLVLDLSGSMRAVMDAPA
                     SELPRRAVPKGLRPTRLDIAKYVMQDFISLRKTDRIGVVVFGKSAYVLSPPTLDYQLL
                     DTLVGKMTLDLIDGTATAIGDALGVAVARLRRSDARSKAVVLLTDGDSNAGAIAPQYA
                     AHLATVVGCRVYTIQIGNGDDVDVQDGVDLFGHPRYVQRRFPVNPELLHEIAKTTGGE
                     AYVATDAKALRESMHDVLDNLEKTRFEASQATYEDLFVLLLLPGVWLVALDALSRAWM
                     LRRFP"
     gene            3672..4757
                     /locus_tag="PK710_07560"
     CDS             3672..4757
                     /locus_tag="PK710_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT09609.1"
                     /translation="MRFAEPWWLLGTAAALVVAAMLIAGGVALVRALRRFGESERVGA
                     LMTEAAGKRRAAKGVLLVFATALAFLAMAQPQYGRGTRLIPATNLDVVIVLDYSKSMY
                     ARDVVPSRISRAKAEVARLVKDLPGARFAAVAFAGEPIGFPLTSDGMAIAQFFRQLEP
                     NDMPVGGTAIARALNRANELLSRDPKSKDHRRVIVLITDGEDLEGSPVNVAKTIADAG
                     TTIHVVQIGSRTPEPVPDVDSTGSVSGHRRGEDGLPLMTSLSAEGEEQLVAIAQAAGG
                     TVIRSERGSTGIDVLSRQLEQMMQSELSEKVETLYADVYFYPLGLALLLLVVETFIGQ
                     AKKRKNTVEIEEKKPPRRSGKGTVGYA"
     gene            4750..5742
                     /locus_tag="PK710_07565"
     CDS             4750..5742
                     /locus_tag="PK710_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT09610.1"
                     /translation="MRSRVGGWLLAAWLVLPGCGMEPDRVFERNAPQVDRAIEALDAG
                     EAGPAAELLQAYLGTGKCEKGEIGVTDLARARSNASFDLGLALFLLGEKFGARFGEEE
                     SSIGDGGPTDEQKQQMELRSEQVTCALKLLRAIGSSADLPVDLAARARYLEGNLEFLR
                     RNYRDAVRAYDEALRLIPGVEPDAGDEVGRDAAWNRAIALRRIEENERDAGQDAQDEQ
                     DASQDTSPDTDSGSPEGGEDGPKESGDEGKQDAPSEDSGGGDGAPDGGDGSAPDDENK
                     QPDAGQPDGDKQPPPQPQTQDDRILDMLEAAPTVQLEDARRNAARRQVRGMVDK"
     gene            5739..7619
                     /locus_tag="PK710_07570"
     CDS             5739..7619
                     /locus_tag="PK710_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOT09611.1"
                     /translation="MTRTFRFAVLVLMAFALLLPAPTVEAQSRPPSLETAVSERETEV
                     GRPFTIQLSAMASATDRTPQNPSLPLPPGIKIRSGPTIMPKTQVTLHGPNLIQQMGIV
                     ATWTLEATRPGQYRIGPPTVIYGGKLHAGAVVDVTVHPPGAGPARPRSRDPFDIFDIF
                     GLPKLPNVFDDPFQPFAEPEPPATDPALAISAAPAQGVFLHAVTDNTSVVLGEQLTLS
                     VYEYYQTAGVRKTSIQEPSAADFLQFPILDPSDDPGVRQADVGSAVWNARLVRKVALF
                     PLRLGDLTIGPMRIGYLGRGLRGEVMRESKPLLIRVQDAPTLGRPMGYRSGDVGNFSM
                     TATVEPRDIDAGGGISVRIKVEGTGNLPSSLTTPSKKGIEWLDPDIKENIEAVDGRIR
                     GERVFSYIVKLHDPGTVDLGQIELPFYDPQRERYGVAKARLGQVTVRQHASAPEIKTD
                     LDRFETVAEPRTALGTAPRISSPITDTLWYWIALFASPLAIVMGGAVWNMAGKARRSV
                     TKWRQSLGRKGKISLQEAKIACKQERFTDAAAEVEKAVHAMIEAATAIKSRGVLRTDL
                     PARLTDVGISDAAARSTVEVLEACETLRYDPSANQQQAIALLNQATELSRLLAVRRKA
                     GK"
     gene            7616..8434
                     /locus_tag="PK710_07575"
     CDS             7616..8434
                     /locus_tag="PK710_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09612.1"
                     /translation="MKPKAFFPGMAILAFCCGLAWAEEPVSHERLFDDGVRALQQGDH
                     NRAITIFETLADRGFVHPDVAYNRGMAYLARIRAKQGRPGDLGRAAAGFEEASLVRPD
                     DEQAIHAAERVRAEVARTTARSGRSQVVVSPSLDRILAGLAPEAVWAWLALVASLTLS
                     TGLVLRQRKASAAHLAGTIATPIGLMALMVFASLAGWSRHIRRSTAPAVVVVAEARIV
                     DEQGTALPKEEPIPEASRVEVLERRAGLSKIRYGQREGWTHTTSLRELARPRAE"
     gene            8628..10043
                     /locus_tag="PK710_07580"
     CDS             8628..10043
                     /locus_tag="PK710_07580"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOT09613.1"
                     /translation="MKRLLALIAGVVLGACSSPACPPPAAAPVCPACQPCDCAASTSA
                     SPLSMAGGDSAGASPEAMVGATLGETADWAQFQRTPSRWGYSSAPALRRPTLRWHKTV
                     GIQGYLNTPLISGPTVFVSSSGERHNRADPLDGIHALDRASGKTLWHAHLPADGNGIA
                     LVGSRLVATCDDGNVYGLDAHNGRIVWERRGQGKMYAHPTIIDDIVVVGDAGGNVRAY
                     GWADGSERWEYGLDGAIRAGLASDGTRIYAVSQGGEVAAFAKNGRLLWKKRVLRPDFG
                     GSTRVPIEGYGAPIVSDSRLIVSFARDTTYDDPAFFALDAPTGAIRWHAKDRSQRTWG
                     NIRSTPTLVNGLMIYGEPYSGDVAALDIFTGTVRYRKTIGGCTFPQYASPASAGDTVY
                     LPRFDGFLYALEALTGRVQWRFYLGEATKAQVSPVVTVGATCSWAPKVGAPLYSPIAI
                     AADGTIIVGNGEGSLFAIAES"
     gene            10150..10950
                     /locus_tag="PK710_07585"
     CDS             10150..10950
                     /locus_tag="PK710_07585"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META and DUF4377 domain-containing protein"
                     /protein_id="HOT09614.1"
                     /translation="MNNHSTKLLLALSFLTVGAGCGNTPPPKEPTASEPSAVAREEQR
                     VEDYEWSLVAAVDGQGKATTAWVVPNQPALSLHFEDQRVTVGNLCNIVSADYSVEGNR
                     LKIGHPVSTKKACDDEALMDLEQRVGQQLPTAQGIELRGGEPPRLTIRWTDGSQWELS
                     GSPTAQKRFGSAPERVFFEVAAEPVPCAQAAQPSCMNVREIRYDDNGIKQSEGPWQTF
                     SGNIEGFSFKPGVRNVLRLHRFTLPNSPANTPAYAYVLDMVVETEMVP"
     gene            11398..11949
                     /locus_tag="PK710_07590"
     CDS             11398..11949
                     /locus_tag="PK710_07590"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HOT09615.1"
                     /translation="MIHHSSKLLWAVSFVMAVVACENASRPKPPMVQEPSAPPNASRT
                     VEDPAQSPDSAVNAAGKIAVEDHDWTLVSSVDAQGKASSAWVVPSRPAISIHFDGRKL
                     MVQNLCNVISADYSVTGKQLKIGSPSATELACQSDILDVEGRVLRLLPTAEAIDLQAG
                     KPPRLTIRWADGSQWELSAKSTP"
     gene            12049..>12678
                     /locus_tag="PK710_07595"
     CDS             12049..>12678
                     /locus_tag="PK710_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09616.1"
                     /translation="MAIQSLRRWRAGLVVAAVLIPLGLWALFERQARRLDALSSHGEP
                     VQAWVTGVSGDKGTTFYAYRVEGKEYSWNVARKDAPYAVGQGFPSLYVPENPSFSQPF
                     ADRALVAQKAAGNRSFAWKVCSGVAVLFFLLAGVAHRDLQRRRAGVVEVLDPGAYKRR
                     LRGAILLFLLVLLLVGGFHFKDALGRGESIVPVLLGLAVGAAIVGGGYVL"
BASE COUNT         2546 a         3484 c         3929 g         2719 t
ORIGIN      
        1 ccgtcaaacc ggtggccacg ctcgagcagc tttcgcaagc ccgtggcgtt gttcgcgaca
       61 tttacgccga tgaacgtgtc aaacaataca tcgtggatgt ggtgcgcgcc acgcgagaac
      121 ccgaacgtta cgggatcaag gatcttgcgg gcctcattca ctacggcgcc agcccccgcg
      181 cttccatcgc ccttgcattt gccggaaaag cacacgcctt tttgcgacgt cgggggcatg
      241 tcgctccgga ggatatcaaa gccattggtc ctgacgttct gcgccatcgc cttgtgctca
      301 cgtacgaggc cgaagcagaa gaagtatcca ccgaagacgt ggtgcgtcgc gtattcgagg
      361 cggttgaggt tccctagggc tacgcattca tgattcccaa ggagttgctc aaacaactga
      421 ggaagatcga gttcgtcacg gcgaaacttg ccaatgaaga actgtcgggc agttatactt
      481 ccgtgttcaa ggggcaaggt ctcgctttcc gcgacgtccg gcaataccag cgcggcgacg
      541 atgttcgcaa gatcgactgg aacgtcaccg cgcgaatgaa tgaaccgttc gtcaaagttt
      601 tcgtggaaga acgagaactt acggtcatgc tggtggtgga cgcctcggga tcgtcgttgt
      661 ttggaaccgt gcgtgccccc aagtcgcaag tggccggcga ggtcgccgcc ctggccgcat
      721 tcagtgccat tcacaacaac gatcgcgtcg ggctcgtgct tgcctccgac catctggaaa
      781 agctcgttcc gccaaaaaaa ggcgaaaaac acgtgctccg ggtcgtgcgc gagatccttg
      841 ccttccaacc ccaaagccac ggaaccaacc tggccatcgc tttcgaaacc ttggtcaaag
      901 tagctcgccg ccgcagcctt gcctttgtca tcagcgattt tttcgatgac ggattcgagc
      961 gcgccatggc tcttgcctcg gcaaaacacg atatcatccc ggtcatgctc gtcgatcctc
     1021 gcgacgaatc cttgcccgac gttgggcttg caacattcga ggatctcgaa tcgggtgaat
     1081 cgatcacggt ggacacgtcg gatgcccgcg tgcgcgagca ttacgcacaa agcatgaaag
     1141 cggtgcgaga cgagcgcatt cgcgtttttc gcaagcataa gctggatttg tgcgtcgtga
     1201 ggaccgatgg ttctttcgta gcgccattgc gcgacttatt cgctcatcgc gccaaaaggg
     1261 ttcgttcgcg atgaaacgtg ggaaggcttt tgggctcctg ctagggctgg gattgacgtt
     1321 aggcggtcaa gcctccgcgc aagatgcttc ttcggaagcg gcagtagacg gctcggtttc
     1381 ggatgcggac gtagcgcgac aacctcgagc cgacgccggt ccatcggagg ctggcaccga
     1441 cgcgctaccg attggggaag atgcagggcg aatctgggcc agttgtgtgg agcacgttcc
     1501 tgaaggagcc acgcgtccca agctgaccaa tacttttcct acgcgggggc tggccggtca
     1561 cgcgttgacg ctggagattc aactcgaaca tggccttggg gaaacggttc ttccgaacgg
     1621 ttttcggatt caagccgata gcgatgccgc gcgcgcgctt tcgagcgccg ggttcgaact
     1681 tcccaatccc aacggcggtt ctcggcccac gctgactcgc cacgaggaac gtgacaaggc
     1741 taccacaaac gttcgtgttc actttttatc acttccggcc aagccgggac gccatgaact
     1801 cacgttgccg ccgatcccca tcgcggtatc tcgagccagc ggtgaagtgc tgacgctatg
     1861 caccacgccg catacgatca tcgtggatga accgattgcg aacgagccgg aagcagcacc
     1921 cctaggcaat cccgaaccga ggccccagcg tgaggtctgg acgctagctc gcgaccttac
     1981 ctacggtgga ttgattggcg tcgtggtagc cgctctggcg ctgtggttca ttttgtggtg
     2041 gcaacggcgc cccaagcctc cgattccccc tccgcctcca cgccccccct gggaagtggc
     2101 tttggaatcg ttgcatcgac ttcgtggcct gcaaccgcac gatgatgcgg agcgggggga
     2161 gcatgtcgat caagtatcgg atattgttcg gatttatctt ggagcccgat tcggtttcga
     2221 cgggatcgag tccacgacgt ccgaaacgct tcgagccttg gagaagattc aaccgccgtt
     2281 gctggtcttt gacgatattc gccaactgct tttcgatagt gatttggtga agttcgcacg
     2341 ctggtcaccg acgaatgaac aatgtgatca actgctggat atggcggaaa aaatcgtaaa
     2401 agcgaccatg cccgcatcga tgccgccttc gtctgctgcg acctctgagg attccaagag
     2461 ttcgaaacag gagggcgaga catgaaaaaa acttcgattt cgccttcttc gactccgaaa
     2521 aagcaacagg ggcgtcgcac cgcgattcgc gtactcattt tgttggctct tctaatcgcg
     2581 gtgatggccc ttgctttcgc gtatccgttg gcgatgcggg gatggaattg gcttcatgtc
     2641 acctggcgtc atccttgggc gttgcttctg ttgttgctgg cgccgatcgt gttatggcgc
     2701 ggaacgctgg gagaggataa ccgtttgccc agaatgcgaa tgggtacgct ccatccattc
     2761 ctgcaagggc cgcagggatg gcgagcgcgc ttgaaagacc ttcccggtat ttttcgtgcc
     2821 gcggcgatca cgatgcttgt gatggctctg gcgcaaccca tctcgatgat gcgtccggaa
     2881 agcacggatg aattgggcat cgacatcgtg ctggtgttgg atctttccgg ttccatgcga
     2941 gcggtcatgg acgcgccggc ttcggaattg ccgcgtcgag ccgttcccaa agggttgcgt
     3001 ccaacccgtc tggatatcgc gaaatacgta atgcaggact tcatttcctt gcgaaaaacg
     3061 gatcgcatcg gtgtcgtggt cttcggaaaa tccgcttacg tcttgtcgcc tcccacgctc
     3121 gattatcaac ttctcgatac gctcgtcggc aaaatgacgt tggacttgat cgatggcacg
     3181 gccacagcca ttggtgatgc ccttggtgtt gcggtagctc ggctacgaag aagcgatgca
     3241 cgttccaaag ccgtggtgct tttgaccgat ggggattcga atgcgggggc catcgccccc
     3301 caatatgccg cgcatctggc aacggtggtg gggtgtcggg tgtacaccat tcaaatcggc
     3361 aacggcgacg atgtggacgt gcaggatggc gtggatttat tcggtcatcc tcgttacgtg
     3421 caacgtcgtt tcccggtcaa tccggagctg ttgcacgaga ttgcgaaaac tacggggggc
     3481 gaagcgtatg tggccacgga tgcgaaagcg cttcgagaaa gcatgcatga cgtgctcgac
     3541 aacctggaaa agacgcgctt cgaggcgagc caggccacct acgaggactt attcgtgctg
     3601 ttgcttctgc cgggagtatg gctcgtggcc ttggatgcct tgagtcgggc ctggatgctg
     3661 cggaggttcc catgagattt gcggaaccct ggtggttgtt gggcacggct gcagcgttgg
     3721 tggtggccgc catgctgatc gccggggggg tggccctggt cagagcgctc cgacgattcg
     3781 gtgaatccga acgggttggg gcgctcatga ccgaagccgc gggaaagcgt cgcgccgcca
     3841 aaggagtgct tctggttttc gctaccgcgc tcgcattcct tgcgatggcg cagccgcaat
     3901 atggaagagg aacgcgtttg attcccgcca ccaatctcga tgtcgtgatc gtgctcgatt
     3961 actcgaagag tatgtatgca cgcgacgtcg taccttcgcg catcagccgc gcgaaagccg
     4021 aggtcgcgcg cctcgtcaaa gatcttcctg gcgctcgatt cgcggcagtg gctttcgcgg
     4081 gcgaacccat cggttttcct ctcacttccg atggaatggc cattgcccag ttttttcgcc
     4141 aactcgaacc caatgatatg cccgtgggag gaaccgccat cgctcgcgct ctgaatcgcg
     4201 ctaacgaatt gctctcccgc gaccccaaat caaaagacca tcgccgcgtc atcgtgctca
     4261 ttaccgatgg cgaagatctc gaaggcagtc cggtcaacgt ggcaaaaacc atcgctgatg
     4321 cgggcaccac catccatgtc gtacagatcg gcagtcgtac ccccgaacct gtccccgacg
     4381 tggattccac cggaagcgtc tcgggacatc gtcgtggcga agacggtctt cccctgatga
     4441 cctccctttc cgccgaagga gaagaacagc tcgtagccat tgcccaggct gcagggggca
     4501 cggtcattcg ttccgagcgg ggctcgacgg gcatcgatgt cttgtcacgt caactcgagc
     4561 agatgatgca atctgaatta tcggaaaagg tagaaacatt gtatgcggat gtatactttt
     4621 atccgctggg gctggcgttg ctgttgttgg tggtcgaaac attcatcggg caggcgaaaa
     4681 aacgcaaaaa cactgtagag attgaggaga aaaagcctcc aaggcgctcg ggaaagggaa
     4741 cggtgggata tgcgtagccg tgtgggtgga tggctgctcg cggcttggct ggttttacct
     4801 ggctgcggga tggaaccgga ccgggtgttc gagcgcaacg cgccgcaagt cgatcgagcg
     4861 atcgaggcgt tggatgcggg ggaggcgggc ccggccgcgg aattgttgca ggcttatttg
     4921 gggacaggca agtgcgagaa aggcgagatt ggcgtgacgg acctggcgcg cgctcgttcc
     4981 aatgcctctt tcgacctggg cctggcgctt ttccttcttg gtgaaaaatt cggtgcacgg
     5041 tttggggaag aagagagttc gattggcgat ggggggccga ccgatgaaca gaaacaacag
     5101 atggagcttc gttccgaaca ggtaacctgt gcgctcaagt tgctaagggc gatcggttcg
     5161 tctgcggatc tgccggtgga cctggcggcg cgagcgcgat acctggaagg aaacctcgag
     5221 tttttgcgaa gaaactatcg agatgcggtt cgtgcctacg acgaagcgct gcgtttgatt
     5281 cctggcgtgg agcccgatgc gggagacgag gttgggcgcg atgcggcctg gaatcgcgcc
     5341 attgcgttgc gtcgcatcga ggaaaacgaa cgtgacgcgg ggcaggacgc ccaggacgag
     5401 caagatgctt cccaggatac gtcgccggac acggattctg gctctccgga aggcggcgag
     5461 gatggaccga aagaatctgg ggacgaaggc aagcaagacg ctccctccga ggattcgggc
     5521 ggtggggatg gtgcgccgga cggtggggat ggctcggctc cggacgatga gaacaagcaa
     5581 ccggatgcgg gacaaccgga cggggacaag cagcctccgc cacaacccca aacgcaagat
     5641 gaccgaattc tggacatgct cgaagcggct cccacggtgc aactcgagga tgcacgaagg
     5701 aatgcggcaa gacgccaggt acggggtatg gtcgacaagt gacgcgcact tttcgttttg
     5761 cggtattggt gctcatggct ttcgcgctgc ttttgccggc gccgacggtc gaggcgcaat
     5821 ctcggccgcc gtcgctcgaa acggcggtaa gcgagcggga aacggaagtg gggcggcctt
     5881 ttacgattca gctcagcgcg atggcgagcg ctacggatcg aacgccccaa aacccatcgt
     5941 tgccgcttcc tcctggcatc aagatacgct ccggacccac gatcatgccc aaaacgcagg
     6001 ttaccttgca tgggcccaat ctcattcagc aaatgggcat cgttgcgacg tggacgttgg
     6061 aggcgacgcg accggggcag tatcgaattg gtccgcccac ggtgatatat ggcggcaagc
     6121 ttcatgcggg agcggtggta gacgtgacgg ttcatccacc gggagcgggc ccagcgcgcc
     6181 cgcgatctcg cgatcccttt gatatatttg acatttttgg ccttccgaag ctcccaaacg
     6241 tcttcgacga tccttttcaa cctttcgcgg agccggagcc tcccgctacg gatccagccc
     6301 tggcgatcag cgccgcgccg gcacaaggcg tttttctgca cgccgtcacg gataacacct
     6361 ccgtggttct cggcgagcag ctaacccttt cggtctatga gtattatcaa acggccggcg
     6421 tacgaaaaac ctcgattcaa gagccttctg ccgcagactt tttacaattc cccatcttgg
     6481 acccgagcga cgatccgggg gttcgtcagg cggacgtcgg cagtgcggtg tggaatgccc
     6541 gcttggtgcg aaaagtagcc cttttccccc tgcgcttggg cgacttgacc attggcccca
     6601 tgcgcattgg atatcttggg cgcggcctgc ggggcgaagt catgcgcgaa agcaaacccc
     6661 ttttgattcg ggtacaagac gcgcccaccc tcgggcgtcc gatgggatat cgctcgggag
     6721 atgttggtaa cttctcgatg acggccacgg tcgaaccgcg ggatatcgat gcagggggag
     6781 gaatctcggt tcggatcaaa gtcgaaggaa cgggaaattt accatcttca ttgaccactc
     6841 cctcgaaaaa agggattgag tggttagatc cggatatcaa agaaaatata gaggccgtgg
     6901 acggccgcat ccggggcgaa cgtgtttttt cctatatcgt caagcttcat gatcctggaa
     6961 ccgtggatct ggggcagatc gagctcccat tctatgaccc ccaacgcgaa cgttatgggg
     7021 tcgccaaagc tcgactgggg caggtcacgg ttcgacaaca cgcgtcggct ccggaaatca
     7081 aaaccgatct cgatcgtttc gaaaccgtgg cagaaccgcg aacggcgctg gggacggcgc
     7141 cgcggatcag ctctccgatc acggatacgc tctggtattg gattgcgtta ttcgcctcgc
     7201 ccctggcgat cgtcatgggg ggagccgtgt ggaacatggc agggaaagcg cgccgttcgg
     7261 tgacgaaatg gcgccaatcc ctggggcgaa agggaaaaat atccctgcaa gaagccaaaa
     7321 tcgcttgcaa acaggagcgt ttcaccgacg ccgcggccga agtggaaaag gctgtgcatg
     7381 cgatgatcga agccgcgacg gcgatcaaat ccaggggagt gttgcgtacc gatcttcccg
     7441 ctcgactgac cgacgtgggt atctccgacg ccgccgcccg tagcaccgtc gaggtgttgg
     7501 aagcctgtga aaccctgcgt tatgacccat cggccaatca acagcaggcc atcgccttgt
     7561 tgaaccaggc gacggagttg agccgtcttt tggccgtgcg gcgaaaggct gggaaatgaa
     7621 gccgaaagct tttttccccg gcatggccat cttggcgttt tgctgcggtc ttgcgtgggc
     7681 cgaggagcct gtttcccacg aacggctttt cgacgatggg gtgcgagctt tacagcaggg
     7741 ggaccataac agagccatca cgatattcga gaccctagcg gaccggggtt ttgtccatcc
     7801 tgatgtggcc tacaatcggg gaatggctta tcttgcgagg attcgggcca aacaaggtcg
     7861 cccgggggac cttggacgtg ccgccgcggg gttcgaagaa gcttccctcg ttcgtcctga
     7921 cgacgaacag gcgattcatg ccgctgaacg ggtacgagcg gaggtggcgc gaacgaccgc
     7981 gcgctctggc cggtcccagg tcgtggtaag cccttcgttg gacaggattc ttgccggtct
     8041 tgcgccggaa gcggtttggg cctggcttgc gttggtggct tctttgacct tgtcgacggg
     8101 tctcgtgttg cggcaacgaa aagccagtgc agcgcacctg gcaggcacga tcgcaacacc
     8161 catcggattg atggcgctca tggtatttgc ttccctcgct ggatggtcga gacatattcg
     8221 acgctcgacc gcacctgcgg tggtggtcgt ggcggaggca agaattgtcg acgagcaggg
     8281 aacggctttg ccgaaagagg agcccatccc ggaggcgtca cgggtggaag tgctggagcg
     8341 aagggcggga ttatcgaaaa ttcgttatgg gcagcgggag gggtggactc atacgacgag
     8401 ccttcgggag cttgcgcgac cgcgggcgga gtgatgcttc cgccacggcg atcccagact
     8461 catattaaca ttgtaaattc aattagttac gcgctatgcc accataggct ccctggcatc
     8521 ggggggctgg ttacggtggt tgagcgctcg gcaatgccag cggtttggcg gggaggaaaa
     8581 ctcgttatcg ccttgttttt tctatttttt tccgcgaata atgcttcatg aaaagactcc
     8641 ttgcacttat cgccggtgtc gtcctcggcg cctgttcttc ccccgcttgt ccgccaccag
     8701 cggcggctcc ggtctgccct gcttgtcagc cttgtgattg tgccgcatcg acaagcgctt
     8761 cccccctttc gatggctggc ggcgactccg ctggtgcttc ccccgaggcc atggtgggag
     8821 caacgctcgg ggaaacagcc gattgggctc aattccaaag aaccccatcg cgttggggat
     8881 actcctcggc tccggccctt cgacgcccca ccctgcgatg gcataaaacc gtgggaatcc
     8941 aaggatatct caacaccccg ctcatcagcg gtcccacggt tttcgtatcg tcgagcggcg
     9001 aacggcacaa ccgcgccgac ccgctcgacg gcatccacgc cctcgaccgc gcctcgggaa
     9061 aaaccttatg gcatgcccac ttacctgctg acggcaacgg tatcgccctg gtcggttccc
     9121 gactcgtcgc cacttgcgat gatgggaacg tgtacggcct ggatgcccat aatggtcgaa
     9181 tcgtgtggga acgtcgtggt caagggaaga tgtacgctca tccgacgatc atcgacgaca
     9241 tcgtggttgt tggagatgcg gggggaaacg tgcgggctta tggctgggca gacggttccg
     9301 agcgttggga atatggtttg gatggtgcga tccgggctgg attggcatcg gatggaacgc
     9361 gtatttatgc ggtatcgcaa ggaggcgagg tggccgcctt tgcgaaaaat ggacgcctat
     9421 tgtggaaaaa acgggtgtta cgaccggatt ttggcggctc cacccgcgtt cccatcgaag
     9481 gctatggagc ccccatcgta tcggactcca ggctcatcgt ttcgtttgcg agggacacta
     9541 cctatgacga tcctgctttc tttgcgcttg acgcaccaac gggtgccatt cgctggcacg
     9601 ccaaagaccg ttcccaacgt acgtggggaa atatccggtc gacaccaacg ttggtgaacg
     9661 gactcatgat atacggtgag ccctattctg gcgacgtggc cgcgctcgat attttcacgg
     9721 gaacggttcg ataccgtaag acgatcgggg ggtgtacttt tccgcagtat gcctcgcccg
     9781 cgagcgccgg agacaccgtg tacttgccgc gattcgatgg attcctctac gccttggagg
     9841 ctttgacggg ccgcgtacaa tggcggtttt atctcggaga agcgacaaaa gcccaggtgt
     9901 ctcccgtggt gaccgtcggg gccacctgct cctgggcgcc taaggtaggt gcgccacttt
     9961 attctccgat agccattgcc gcggacggaa cgatcatcgt gggtaacggg gaaggcagtc
    10021 tgttcgcgat tgccgagtca tgaaacccaa ggagtcgtgc cttcctctca ttaaatcacg
    10081 aaaaaaatcg caataggttt ggacagcagt agcaaccatg gtttgccgtc ccgcatccaa
    10141 cggggcacca tgaacaatca ttccacaaaa ctacttctgg cattgtcttt tcttactgta
    10201 ggggccggtt gtgggaacac accaccgccg aaagagccaa cagcttccga gccgtcagcc
    10261 gtcgctaggg aagagcaacg ggtggaggac tacgagtgga gtttggtggc cgcggtagat
    10321 gggcaaggga aggcgacaac cgcctgggtc gttccgaatc aaccagcgtt atcgctccat
    10381 ttcgaagatc agcgggtgac ggtaggtaac ctatgcaaca tcgtgagcgc cgactattcg
    10441 gtggaaggga atcggctgaa gatcggccat ccggtgtcga ccaaaaaagc ttgtgatgac
    10501 gaggcgttga tggatttgga gcagcgggtt ggtcagcagc ttccgacggc gcaagggatt
    10561 gagcttcggg gtggggagcc gccccggctt acgattcgct ggacggatgg gagtcaatgg
    10621 gagttgagcg gaagccccac ggcgcaaaaa cgattcggta gcgcaccaga gagggtattt
    10681 ttcgaggttg cggcggagcc ggtgccttgt gcgcaggcag cgcaaccctc gtgcatgaac
    10741 gtgcgtgaga ttcgttacga cgacaacggc atcaaacaaa gcgaggggcc ctggcagacg
    10801 ttttctggga atatcgaagg gttttccttc aagccggggg tgcgaaatgt gcttcgcctc
    10861 catcgattta ccttacccaa ttcccccgcc aacacgcctg cctacgccta tgtgctcgac
    10921 atggtggtgg aaacggagat ggtgccatga tgctgccgcg atggcgcacg accgcttgcc
    10981 tcgaggattc cgcgattcgg tgacgcgggc ccaaggctcg aaaccacttt ccacaaatct
    11041 tagtaattcc taatagttat agaaatatta ccgcctccta tcccgtcgcc tccgcttctg
    11101 agccgccaaa gtggaatgga cagccccaag cgcagaacgc caatcgcgtc aaagcagcca
    11161 ggtgacaata ttgggctctt cgcgataccg gaggcgctgg ttcgtcacgc gccgccccat
    11221 tctccatcgc gacagttttc gacgacgcca tcaacgggaa caatggaaat gaatttttcg
    11281 atttcagagc acggattgcc acctgttccc ggatttgata cgagttttct ttgactcgac
    11341 agattcgtag gctgggtttg caaccctttt tcgcgttcgc gcatgcagcg gtgccacatg
    11401 atccatcatt cctcaaaact tctgtgggca gtgtcttttg tcatggcggt tgtggcttgt
    11461 gagaacgcct ctcgaccgaa accgcccatg gtccaagagc cttccgcccc acccaatgca
    11521 tctcgaaccg tcgaagatcc tgctcagagc cccgactccg ccgtgaacgc ggcaggcaag
    11581 atagccgtcg aggatcatga ttggacgttg gtctcgagcg tggatgcaca aggcaaggct
    11641 tcgagcgcgt gggtcgtgcc gagccggccg gctatttcga tccatttcga cggtcggaag
    11701 ctgatggttc agaatctgtg caatgtgata tccgccgact attcggtgac aggaaagcaa
    11761 ttgaagattg gctccccgag cgcgacggaa ctggcctgcc agagcgatat cctcgacgtg
    11821 gagggaaggg ttcttcggct gcttccgacg gcggaggcta tcgatcttca ggctgggaaa
    11881 ccgcctcggc tgacgattcg ttgggcagat gggagtcaat gggagttgag cgcgaagtcc
    11941 acaccgtaaa acgcgggttc gtggttcgcc aaaacggtcc cgcgcaaagc gcggcatgga
    12001 gcaagctcgg ggattatggt ggtagacgga aacctacaga aatcgtcggt ggccattcaa
    12061 tcgctccgac ggtggcgtgc gggtttggtc gtggcggccg tgctcatccc tctcgggttg
    12121 tgggcgctat tcgaacgtca ggcacgtcgt ctcgatgcgc tttcttccca tggcgagccg
    12181 gtgcaggcgt gggtcacggg ggtatcaggc gacaagggga caacatttta cgcctaccgg
    12241 gtggagggca aagagtatag ctggaatgtg gcgcgcaagg atgcgcccta cgctgtcggg
    12301 caggggttcc catcgctcta tgtacctgaa aatccatcat tttctcaacc ttttgccgat
    12361 cgggctctgg tggcccagaa ggctgctggc aaccgaagct ttgcctggaa ggtttgttct
    12421 ggcgttgccg tattattttt tctgttggct ggggtggcgc atcgtgacct gcagcgtcgt
    12481 cgggctgggg tagtggaggt gctcgatccc ggcgcttaca agcggcgtct tcgcggtgct
    12541 atcttgttgt ttttattagt tttattgttg gtggggggat ttcatttcaa ggacgctttg
    12601 gggcgggggg agtcgatcgt tccggtgctc cttgggttgg ccgtgggagc agcgatagtg
    12661 ggtgggggtt atgtcctt
//
LOCUS       DAOSOR010000100        12664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4960_length_12664_cov_3.914426, whole genome shotgun sequence.
ACCESSION   DAOSOR010000100 DAOSOR010000000
VERSION     DAOSOR010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 12664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12664
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4960_length_12664_cov_3.914426"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /locus_tag="PK710_07600"
     CDS             complement(<1..1446)
                     /locus_tag="PK710_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT09617.1"
                     /translation="MGQQLNPPSTAKRCKLCGQRFTNDAFFCPFDGEPLEVAPEAFSP
                     SDDPLVGCIIDDRYKILDYLGEGGMGTVYRVEHRTLQRQFAMKVLRCDLAKEPELGAR
                     FIQEARAAATVHHPNVIQITDFGQLPEGIPYFVMEYLTGRTLAAVLQKGGPLPATRAV
                     RILLQAAAGIGAAHNAGVIHRDLKPDNIFLLNALGAEETVKLLDFGVAKVAGAARITQ
                     TGIVFGSPHYMSPEQASGQPVDHRADIYALGIVMYELFTGHLPFEADTFMGVLTKQMF
                     VTPEPFNVKSGSARELGALEDITFRCLEKKTENRYASVEELIADIEEVVEIGNEDSFR
                     VKASASSFSIRTPTEFRLSDELELPSAEELAALRGSQTQQNSRAFWAILAFAAAGLLV
                     AIAITAFLWINKARYESSEAYATPTSTPSDLPANSVSVQQSPPQTPAEPAITPAIPDN
                     SISVPTTRPTTPRSRHSTIPSPPKPSTSEPVD"
     gene            complement(1513..2751)
                     /locus_tag="PK710_07605"
     CDS             complement(1513..2751)
                     /locus_tag="PK710_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOT09618.1"
                     /translation="MGIMDFVKGGVKEMMIARPDEKKSLVVYKHPDQNIPFWSQLTVD
                     SDEAAVFFKDGRVVGILPPGRHTLQTQNIPFLNNLVNQFTGGNVFIAEIFFVKTQPVR
                     DIPFGGPIGMMIDPLTQEMCTPRMFGKFSLVVVDPARFVVGYTGQAAAGDNDQTLEWV
                     KGLFFLGVKTVIGELCEAEGKSIIQAVALTQQLAQRFVERAPSLEDIGVRVLQMGNFN
                     INFDPEEKQRLVEAQAEVAKAQRGIRIAKAEAEARQHKLDQDFQQDARYVQQVAGSWQ
                     NYAGGKAMIGAGEGMAKGGGDASLAGVGAQMAVGVGMAGMMAGQMAHPQGPQYPQPNM
                     PPPQGQYGQPPPYPQAAVPTAAGGTTQCPKCAATVAVGRFCAECGAPLAAPAKKFCSG
                     CGTELAPTARFCANCGTPAA"
     gene            2911..4035
                     /locus_tag="PK710_07610"
     CDS             2911..4035
                     /locus_tag="PK710_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09619.1"
                     /translation="MPTPIDLSRLHPMARKALEAAAPKKLRLGAARGLLPGIPPADIL
                     TVVALLSQEADSPVSTTARTTLAQFPPAILDAALNTELAGVVIEALARSNPGQDSLLT
                     RLLDNAGIAPETVAFLAEHGSESLCEHIAINEQRALHHPIIIEKLYMNEKARSSTAER
                     LLDLAVRNGVELNIPAFKEAAAAIQNELIIERSEEPTPDDILFRQVGELAEQSGIDSE
                     TEDVVEIDDEGNEVIKPQAQPLWVQIGNMTVSQKIRRALLGTGTERMLLVRDTNRLVA
                     AAAIRSPKVKENEVAQISSSRAVSDEVLRIIATNREWTHNHQIKYNLVCNPRTPFTFA
                     AQLVPHLREHELKSLAKSKNVTGAVAQAARQHLMKKQRRG"
     gene            4057..4608
                     /locus_tag="PK710_07615"
     CDS             4057..4608
                     /locus_tag="PK710_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09620.1"
                     /translation="MSLPTLEAFLNVAAQTVTLHETGPEAPCALTQWLAKTVPWIQVH
                     ATQGPAPRTLCLRGPNSHGTIQFVGDLAHRMIEPLAITVRALGTGQVDLDTPATPVFL
                     SELVRPVHLRLVVSVACPYCPASAAVVLRLACITDKVHVQIIRADLEDAPRVHAVPTL
                     FVGNEELLTGPMAEMAVVEAIAR"
     gene            complement(4645..6408)
                     /locus_tag="PK710_07620"
     CDS             complement(4645..6408)
                     /locus_tag="PK710_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT09621.1"
                     /translation="MADESQRARMMELAQQVAKLQDKLNKQQKINRVLMSRVERSMDA
                     QGDAFSLFQTAISLEAKLKERTVRLQRAMQDLERTNRELEQARDDAEAANRAKSEFLA
                     NMSHELRTPLNAIIGYSEMLIEEAEDVALDGFIPDLQRVRSAGKHLLGLISDILDLSK
                     IEAGKMDVHLESFDVLAMLSDVQSVIEPLVAKNANRFVLSCDDSIASMHSDIIKVRQT
                     LLNLLGNACKFTNDGVITLDVRREPSATGDVMVFTISDTGIGMSAEQTKKLFQPFSQV
                     DASTTRKYGGTGLGLAITRHFARMLGGDVQVESELGKGSTFVLRIAANVPETTKRPSL
                     SPSSLKDSNNPRVLVIDDDTRMHDLFDELLVPQGFSVLHAFNGSSGIRVAQEQVPDLI
                     TLDIVMPDKDGWSVLAELKKIPILSHIPVVLVSMHAERDLGFALGAADFVSKPLEAKA
                     LLNTLRRHRRKDRPAHALVVDDDEPTRQMLRRILEKEGWQVSEADNGRTALESLENWT
                     PGLMLLDLMMPEIDGFSVVEAMQARPEWRKIPVVIVTALDLSQADLDRLGGRIFKVLQ
                     KGKYNRADLLELVRQRMNSRK"
     gene            complement(6432..7571)
                     /gene="nosP"
                     /locus_tag="PK710_07625"
     CDS             complement(6432..7571)
                     /gene="nosP"
                     /locus_tag="PK710_07625"
                     /inference="COORDINATES: protein motif:HMM:NF041558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035991 - nitric oxide sensor activity
                     [Evidence IEA];
                     GO_process: GO:0046209 - nitric oxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing protein NosP"
                     /protein_id="HOT09622.1"
                     /translation="MKTEGIRNGFSTNSSAQEAIDEIWEQTTQPDAKLTVLYCSPSYD
                     LTALARRVRERFADSTVIGCTTAGEFGPGGYLDQSISAFSIGGPTFEAVTGTLDDLQR
                     FDIPEGHGLTRKLLQELRAKGVQPSSRNTFGYLLIDGLSIREEIVASALHGSMNDIPL
                     FGGSAGDRLRFEKTMLFSNGEFLSDRAILSLVTTTRPFEVFKKQHFVESSVKLVITEA
                     DPARRLVTEINGEVACREYADAIGKDVQELGPDVFAAHPLVLSISGNSYVRSIQKVNE
                     DGSLSFYCAIDEGIVLRVAEGKDIFNHLREALEDIRRSIGEPALIIGCDCVLRHLEYE
                     QAGLKEAVGNLLRAYHVVGFSTYGEQFNAMHVNQTFTGVAIGDAG"
     gene            complement(7807..9351)
                     /locus_tag="PK710_07630"
     CDS             complement(7807..9351)
                     /locus_tag="PK710_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09623.1"
                     /translation="MADALVTRATNRRYPVFIASSYGYPRWEKTVEPSPTIAPLLTID
                     NRIRRCYAKGVGRKRLIPLLFALLVGACAEQDPLPITLVVTSGQEKDAFIQAPAPERF
                     TLRLISVEGAPSQLLQAPWPSQPITLEGLDTNQLAAFEGIGSDIDDVPVMRGATILHP
                     LWAVGAQSLPLFMGRVGKTSRPDGVLPFSHVHGLSAIVSARFIVSAGGTEAYRTDGSS
                     SDPAAFSVYDVGLWQASTATGTLPRMPRAMAMVQGRWALVIEEQGASWFDFSTFESTE
                     VAAPEALTFADIAGGQTVYGEQGEAYVVGPTRKDLPSNIVLRVDWQGTLTVIRLGVPR
                     VAVAATYVKGRGLLLVGGHPSEPGALLLAPGVNSFSPLAYPPDPVQGAMATSLDDERV
                     VLAGGAIEGTSAPTRILDLRCSTQCHSVDLAPSVKPIAIVHGLAHTTRADEVLLVGED
                     GSDSTTKLVRIAELDGVAFSESVVLHEPRVGAASVPLFGRMVAIVGGRKLDDSGVPSV
                     EVYIPK"
     gene            complement(9393..9887)
                     /gene="nikR"
                     /locus_tag="PK710_07635"
     CDS             complement(9393..9887)
                     /gene="nikR"
                     /locus_tag="PK710_07635"
                     /inference="COORDINATES: protein motif:HMM:NF001884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOT09624.1"
                     /translation="MPSRASASREPVARFGVAMPHDLLERFDHYVQRKGYTNRSEALR
                     DLVRVELASEAWDRGEDVVAAITMVYDHHIPGLTDKLVSIQHGSGGHVISTMHVHLDH
                     SHCLEIIVARGPARDLEKMADEIVRTRGVLVGRIVPALSMHALGDSFLHEHTHDHSHD
                     PSHG"
     gene            complement(9891..10667)
                     /locus_tag="PK710_07640"
     CDS             complement(9891..10667)
                     /locus_tag="PK710_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOT09625.1"
                     /translation="MEHGSLRNLDQIQEGDARRGGSRVGALVLASIAGACLVFAVFAL
                     VKRPQFGNEPQVDPLAELAGKSSQGQGVPALDGNQMNFPTMLSDDPNPTTAMIALRAA
                     SSSSAQPPFVLPPGMPTVPPTAGDSLPVVPLPAQAYVSPSPIVAEPRDSLTAMAASAA
                     NPRGEAVAKGQPGGYQLQVSSYRTEKEAEAFASRLRRGGHHAYVESALVPGKGTWYRV
                     RIGPFKSRWDAIRYRKEFEQREHIVGFLVEPPKKTAVRGD"
     gene            complement(11056..12180)
                     /locus_tag="PK710_07645"
     CDS             complement(11056..12180)
                     /locus_tag="PK710_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09626.1"
                     /translation="MGAGRLWIALPLALLFSCGSDNEDSSPRDGYDAGTDSCVSVETG
                     TDAVSEVGQEDTSIDSNPVDGPADTAVDTNDEVLLPDGSEDSTPDAMTEAMALESYAE
                     AFCDLMQRCAPYMVSYLYGDETTCVARYVAGGRSVVLPDGPGVQKNVADVVSCAMNMS
                     NMTCPELYAGGRTTACPMVVGTLTEGASCFSDLQCTSGFCDRLGPCGSCAPPIGVGGQ
                     CEFANDGCAPDLICHETSFADPALCKQSAGQGESCGDTVICRGNLRCRQGKCEQPGGL
                     GDPCDSSTGSCNGLLGLRCSGKVCVAGTPMADVGGTCGIQGNTFIPCKGTSYCDSTTH
                     RCVAKLDDGSPCEASSANPCLYFASCQNGVCTVDAEPSCP"
     gene            12308..>12664
                     /locus_tag="PK710_07650"
     CDS             12308..>12664
                     /locus_tag="PK710_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09627.1"
                     /translation="MVDVEVDWIRLGREGSAMKPRQQRFVDLSILATRGVYAWTIVML
                     LLAMALIGCGADTSDREGPPGTGGAYGSGGHTSDSGSDTGICPPGTFGPTCQPCVAGQ
                     YCAGGEAAPVDCGSGIY"
BASE COUNT         2779 a         3566 c         3624 g         2695 t
ORIGIN      
        1 gtccacaggc tccgatgtag agggttttgg gggtgatggg atggtggaat gccgagagcg
       61 tggagtggtg gggcgggtgg tcggcaccga gatgctgtta tcaggaatgg caggcgtgat
      121 cgccggttcg gccggggttt ggggtggaga ttgctgaacg gaaacgctat tcgcgggaag
      181 gtctgaaggg gtagaggttg gtgtggcgta agcttccgag gattcgtatc ttgccttgtt
      241 gatccataga aaggccgtga tggcgatggc cacgagcaat ccagcggcag caaaagccaa
      301 aatcgcccag aacgcacggg aattctgttg ggtttgggag ccacgaaggg cggctagttc
      361 ttctgcggag ggcaattcga gttcatccga aaggcgaaat tcggtgggag tacgaatgga
      421 aaaggaggac gcggatgctt tgactcgaaa ggaatcttca tttccgattt cgaccacttc
      481 ctcgatatcg gcgatgagtt cttctacgga ggcgtatcga ttttccgttt ttttttcgag
      541 gcaacgaaag gtgatatcct cgagggctcc gagttctcgg gccgaaccac ttttgacgtt
      601 gaatggctcg ggggtcacga acatttgctt cgtaagcacc cccatgaagg tatcggcctc
      661 gaacggtaga tgccctgtaa acagctcata catgacgata ccgagggcat agatatcggc
      721 gcgatggtcg acgggttggc cggaggcttg ctctgggctc atgtagtgcg gagagccgaa
      781 aacgatgccg gtctgtgtga tgcgagcggc gccggcgact ttggctacgc cgaagtccaa
      841 gagtttgacg gtttcttccg cgcccaaggc attgagaaga aagatattgt cgggcttgag
      901 atctcgatga atgacccccg cgttatgggc ggcaccaatt ccggcagcag cttgaagcaa
      961 gatacgaaca gctcgcgtcg cggggagcgg cccccctttc tggaggaccg cggccaacgt
     1021 acgccccgtc aggtattcca tgacgaaata cggaatccct tcgggcaatt gtccgaaatc
     1081 ggtaatctgg atgacattgg ggtggtggac cgttgcggca gctcgagctt cttgaatgaa
     1141 ccttgcgcca agctcgggtt ctttcgccaa gtcacaccga agaaccttca tggcaaactg
     1201 gcgctgaagg gttcggtgtt cgactcgata caccgtaccc atgccgccct ctccgaggta
     1261 gtcgaggatc ttgtaacggt catcgataat gcacccaacg agcgggtcgt cagaagggct
     1321 aaacgcttcg ggggctactt cgaggggttc cccatcgaag gggcaaaaaa acgcgtcatt
     1381 ggtgaatcgt tgtccgcata gtttgcagcg ttttgctgtc gatggaggat tgagttgttg
     1441 acccacgtcg tgattgtaaa ccaaaaggtc aggtcaagcg ctatgggaag aggggaggaa
     1501 ggagggtcgg gctcaggcag cgggcgtacc gcaattggca cagaagcgag cggtaggggc
     1561 gagttccgta ccgcatccgg agcagaattt tttggcgggt gcagcgaggg gcgctccgca
     1621 ttcagcacaa aaacgcccga cggccacggt agcggcgcat ttggggcatt gggtggtgcc
     1681 cccagcagcc gtcggcaccg cggcttgcgg gtaaggcgga ggctgaccgt attgaccttg
     1741 ggggggtggc atattgggct gggggtattg aggtccttgg gggtgggcca tctgaccagc
     1801 catcatgcct gccattccga ctccgacggc catttgtgct ccgactccag ccaaactggc
     1861 atccccaccg cctttggcca tcccttctcc ggcgcctatc atcgcctttc ctccggcgta
     1921 attttgccag gatccggcaa cttgctgcac gtaacgagca tcctgctgga agtcttggtc
     1981 cagtttatgc tgtcgagcct cagcttctgc tttggcaatt cgaataccgc gctgtgcttt
     2041 ggcgacttcc gcctgcgctt cgacgaggcg ctgtttttct tcggggtcga agttgatatt
     2101 gaagttgccc atttgcagca cccgcacccc gatgtcttcg aggcttgggg ctcgttccac
     2161 aaagcgttgg gctagttgtt gggtgagggc gacagcttga atgatgcttt ttccttcggc
     2221 ttcgcaaagc tctccgatga cggttttgac cccgaggaaa aacaggccct tgacccattc
     2281 cagcgtttga tcgttgtccc ctgcagcggc ttgaccggta tagccgacga cgaaacgagc
     2341 ggggtcgacc acgacgaggg aaaactttcc gaacattcgt ggggtacaca tttcctgggt
     2401 gagcgggtcg atcatcatgc cgatcggtcc cccgaagggg atatcgcgca ccggttgtgt
     2461 tttgacgaag aagatttcgg cgatgaagac gttaccgccc gtgaattggt tcacgaggtt
     2521 attgaggaaa gggatgtttt gtgtctgcaa ggtatgacgg cctggaggca aaatgccaac
     2581 gacccggcca tctttgaaga acacggccgc ttcatccgaa tccacagtca gttgggacca
     2641 aaaggggatg ttttggtctg ggtgtttata aacaacgaga ctttttttct cgtcggggcg
     2701 ggcgatcatc atctctttga cgccaccctt cacgaaatcc atgatcccca tgcgttacct
     2761 ccaggtcggt gatcactgcg gttgcgcttg cctcttatga ggcgtctcaa gataatgcct
     2821 gatcgccact tcggcaatgc caagtcagac ccatactcat ggttttggga acacagcggc
     2881 aatttgtcct gctacgctgg aaataccgtc atgcctacac cgattgattt gtcacgtctt
     2941 cacccgatgg cgcgcaaagc gctggaggct gccgcaccga aaaagcttcg cctcggtgcc
     3001 gcgcgcggtc ttcttcccgg cattcctccc gccgacatcc tcacggtcgt agccttactt
     3061 agccaggaag cagattctcc ggtttccacc acggcgcgca cgacgctcgc ccaattccct
     3121 cccgccatcc tcgatgcggc gttgaacaca gagcttgcgg gggttgtgat cgaagcgttg
     3181 gctcgcagca acccaggtca ggactcgctg ctcacgcgcc tgctcgacaa cgccggtatc
     3241 gcccccgaaa ccgtcgcctt tctcgcggaa cacggctccg aatcgctgtg tgaacacatc
     3301 gccatcaacg aacagcgtgc tttgcatcat ccgatcatca ttgaaaagct ttatatgaac
     3361 gaaaaagctc ggtcttccac cgccgagcgc ttgctcgacc ttgcggttcg caacggagtg
     3421 gaactcaata tcccggcatt caaagaggcc gcagcagcga ttcagaacga gttgatcatc
     3481 gaacgcagcg aagaaccgac ccctgatgac attctttttc gacaagtggg cgagcttgcc
     3541 gagcaatccg gaatcgattc cgaaacggaa gatgttgtcg aaatcgatga tgagggcaac
     3601 gaggtcatca aaccgcaagc ccaaccgttg tgggtacaga ttggaaatat gaccgtcagc
     3661 cagaaaattc ggcgcgcgct actcggaacg ggtacggaac ggatgttgct ggtacgcgat
     3721 accaatcgtc tggtagcggc ggcagccatc cgaagcccca aggtcaaaga aaatgaggtg
     3781 gcgcagatca gttcgagtcg cgccgtatcc gatgaggttc tgcgcatcat cgccaccaac
     3841 cgggaatgga cccacaacca ccaaatcaag tacaacctcg tttgtaatcc tcgcacgcca
     3901 tttactttcg ccgcgcaact cgttccccat ttgcgggaac acgagctgaa atcgcttgca
     3961 aaaagcaaga acgtcacggg cgcggtggcc caagccgctc gtcaacacct gatgaaaaaa
     4021 cagcgtcgag gctaggaaac gaaagctgtc cacggcatgt ccctgcccac actggaagcg
     4081 ttcttgaacg tcgcagccca gacggtaacg ctacacgaaa cgggtcccga agctccttgt
     4141 gccttgacac aatggttggc aaaaaccgtc ccctggattc aagtccatgc tacccaaggc
     4201 cccgcccctc gcacgctctg tttgcgtggc cccaacagtc acggcacgat ccaattcgtg
     4261 ggagacctcg cccatcgcat gatcgagcca ttggcaatta ccgttcgcgc gctgggtacc
     4321 ggacaagtcg atctcgacac accggccaca cccgtatttc tttccgaact cgtgcgtcct
     4381 gtccatcttc ggctcgtcgt ctccgttgcc tgcccctatt gtcccgcatc ggcagccgtc
     4441 gttctacgtc ttgcctgcat cacggataag gtacacgtgc agatcattcg cgcggatctc
     4501 gaagatgcgc cgcgcgtaca tgcagtaccc acgctatttg tgggcaacga agagctactt
     4561 acggggccga tggcggaaat ggccgtcgtc gaagctatcg cacgataggc gggttagacc
     4621 taaccgatcg ccacgcattg tggattattt tcggctgttc atgcgttgac gaacgagttc
     4681 cagcagatcc gcgcgattgt attttccttt ttgcaatacc ttgaagatcc gaccgcccaa
     4741 tcgatcgaga tcggcctgag acaggtcgag agccgtcacg atcaccacgg ggatcttgcg
     4801 ccattccggt ctcgcttgca tggcctccac cacggaaaaa ccatcgatct cgggcatcat
     4861 caagtccagc aacatcaagc cgggggtcca attttcgagc gattcgaggg ccgtacgacc
     4921 attgtccgcc tcggagactt gccacccttc tttttcgagg attcttcgaa gcatttgtcg
     4981 agtcggctca tcatcgtcca ccaccaaagc atgcgcgggg cggtccttac gacggtggcg
     5041 tcgaagggta ttcaacagcg ctttggcttc gagcggtttg gagacgaagt cggcggcgcc
     5101 gagtgcgaaa cccaggtctc gttccgcgtg catggacacg agtaccacgg gaatgtggct
     5161 gagtatggga atttttttga gttcggcgag tacggaccac ccatctttgt ccggcatgac
     5221 gatatcgagg gtgatgaggt cgggcacctg ttcttgggcg actcggattc cgctgctccc
     5281 gttgaacgca tgcaatacgg aaaacccttg gggaacaagg agttcgtcga ataggtcgtg
     5341 catccgcgtg tcgtcatcga tgaccaacac gcgcgggtta ttggagtctt tgagggagga
     5401 gggagaaagg gagggtcgtt tcgtcgtctc cggcacattg gccgcgattc gcaacacgaa
     5461 ggtgcttcct tttccgagtt cgctttcgac ctgcacatct ccaccaagca tccgtgcaaa
     5521 gtggcgcgta atcgccaatc ccaaccccgt tccaccatac ttgcgcgtcg tggacgcatc
     5581 gacttgagag aagggctgaa acaacttttt cgtttgctcg gcactcattc cgatacccgt
     5641 atcggagatg gtaaacacca tcacgtcacc ggttgccgaa ggctctcttc gaacgtcgag
     5701 cgtaatgacc ccatcgttgg tgaacttgca agcgtttccc aacaggttca acagcgtttg
     5761 tctcactttg atgatgtccg aatgcatgct ggctatgctg tcatcacacg acagcacgaa
     5821 gcggtttgcg ttttttgcca ccaacggttc gatcacggat tgcacgtcgg acagcatcgc
     5881 caaaacgtcg aagctctcga ggtggacatc catttttccg gcttcgattt tcgataggtc
     5941 caggatgtcg gagatgagtc cgagcaggtg ttttcctgcg gatcgcacac gctgaagatc
     6001 ggggatgaag ccatccaacg caacatcctc cgcttcttcg atcagcatct cgctatagcc
     6061 aatgatggca ttgaggggcg ttctcagttc atggctcatg ttcgcaagaa actcggattt
     6121 tgcccgattc gcggcttctg catcgtcacg agcttgttcg agttctcggt tggttcgctc
     6181 gagatcctgc atggcccgct gcaaacgaac cgttcgttcc ttgagctttg cttccagcga
     6241 aatagccgtc tgaaataggg aaaaagcgtc cccctgcgcg tccatgctcc gttcgacacg
     6301 actcatgagc acacggttga tcttctgttg tttgttcagc ttgtcctgga gcttcgccac
     6361 ttgttgggcc agctccatca tgcgagcgcg ttgcgattca tcggccatca caacctccgt
     6421 ggggttccca cctagccagc atccccaatg gccacaccag taaatgtctg gttgacgtgc
     6481 atggcgttga attgctcgcc gtaggtactg aacccaacaa cgtgataagc tcgaagcagg
     6541 ttgccgacag cctctttgag tcctgcctgt tcgtactcga gatggcggag aacacagtca
     6601 catccgatga tcaaggcagg ttcgccgatc gaccgtcgga tgtcctcgag ggcctcgcga
     6661 aggtggttga agatgtcttt gccttccgcc accctgagaa cgattccctc atcgatcgcg
     6721 caataaaacg ataagctacc gtcttcattg actttttgta tggatcgaac ataggaattg
     6781 ccggagatgc tgagcacgag cgggtgggca gcgaacacgt caggaccgag ttcctgcaca
     6841 tccttgccaa tggcatcggc gtattctcgg catgccactt caccattgat ctcggtaacg
     6901 agacgacgcg ctgggtcggc ttcggtgatg acgagtttga cggacgattc gacgaagtgt
     6961 tgttttttga agacttcgaa cgggcgcgta gtggtcacca gcgaaaggat ggcgcgatcg
     7021 gaaagaaact ccccgttgga aaataacatc gtcttttcga aacggagcct atctcccgcg
     7081 gagcctccaa ataacgggat gtcgttcatc gaaccatgca acgcgctggc tacgatttct
     7141 tcccgaatcg aaaggccatc gatcaacaag taaccaaacg tgttgcgaga agacggctgc
     7201 acgcctttgg cacgtagttc ttgcagcagc tttcgggtca gcccatgccc ctccggtatg
     7261 tcgaatcgtt gcaaatcgtc caatgttccc gtcacggctt cgaatgtcgg accgccaata
     7321 ctaaaggcac tgatggattg gtcgagatat cccccgggcc cgaattctcc cgcggtcgta
     7381 catccaatga cggtagagtc agcgaagcgc tcgcgcacgc gacgagccaa tgccgtcaaa
     7441 tcatagcttg gagaacaata caaaacggta agcttggcat ccggttgggt ggtctgttcc
     7501 catatctcgt cgatggcctc ctgagcggat gagttcgtgg agaagccgtt tcgaatgcct
     7561 tccgttttca tgatctcaac ctatttgttc ttccggacgt gcggttgata acgataacgc
     7621 agaacacaag cgaggcacag gaagaagcct gatttgcagg catgcggggc tacgtggacg
     7681 gttttcacgg agcagcaaca aggccaattc gagtggaagc cggacttgca ggccgtagcg
     7741 cgatgcaaaa cgaggacacg ggaagaccaa aacgcggtgg gcgcaataac cacgcgtaaa
     7801 aattaatcat ttcgggatgt acacttccac ggaaggcacg ccggaatcgt ccagttttcg
     7861 tcctccgaca atggcgacca tgcgcccgaa aagcggtaca gaggcagcgc ccacgcgagg
     7921 ctcatgcaaa acaaccgatt ctgaaaaggc aacaccgtct agctccgcga tacgcaccag
     7981 tttcgtagtc gagtccgagc catcctctcc cacgagcaac acctcatcag ccctggtcgt
     8041 atgagccaat ccatggacga tcgcgatagg tttcacggaa ggagccaaat cgacactgtg
     8101 gcactgcgtg gagcatcgca aatccaggat tcgagttgga gcagacgtac cctcgatcgc
     8161 tccacccgcg agcaccacac gctcgtcatc gagcgaggtt gccatcgcgc cttgcactgg
     8221 gtccggggga taagcaaggg gtgaaaacga gttgacaccg ggagcaagaa gcaatgctcc
     8281 cggctcgctc ggatgccctc ccacaagcaa taatccgcga cccttgacgt aggtggcggc
     8341 cacggcgacg cgtggaacgc ccaggcgaat cacggtcagc gttccctgcc aatccacacg
     8401 aagcacgatg ttcgacggga gatctttcct cgttggccca accacatacg cctccccttg
     8461 ctcgccatac acggtttgac caccagcaat atcggcaaac gtcagcgctt cgggtgccgc
     8521 aacctccgtg gactcgaagg tgctgaagtc gaaccagctt gctccttgct cctcgatcac
     8581 caacgcccaa cgtccctgaa ccattgccat ggcccgaggc attcgaggta gcgttccggt
     8641 cgcggtcgac gcttgccaca atcccacgtc ataaacggaa aatgccgcgg gatcggagga
     8701 ggaaccatcg gtacgatacg cttcggttcc tccggcggag acgatgaatc gcgcgctcac
     8761 gatcgcggac aagccatgga catgggaaaa aggaagtacg ccgtcaggac gggaagtctt
     8821 tcctacgcgt cccatgaaaa gaggaagaga ctgggctccc acggcccaaa gcggatgaag
     8881 gatcgtggcg ccgcgcatga cagggacgtc gtcgatatca cttccaatac cttcaaaagc
     8941 cgcgagttgg ttcgtatcca agccctcgag ggtgatgggt tgggaaggcc atggcgcttg
     9001 aagaagttgg gaaggcgccc cttcgacgga tatcagacgc agcgtgaaac gttctggagc
     9061 gggagcctgg atgaaagcgt ctttctcttg tcccgacgtt acaacgagtg tgatggggag
     9121 cggatcttgt tctgcacagg cgccaacaag cagagcaaac agcaggggaa tcaatcgctt
     9181 tcgacccaca cctttggcgt agcaacgcct gattcgattg tcgatagtca gcaagggagc
     9241 gattgtcggt gacggttcaa cggtcttttc ccagcgtgga taaccgtacg aagaggcgat
     9301 gaatacgggg tatcgacgat tcgttgcgcg cgtcacgagc gcatcagcca ccgcaaaggc
     9361 ttacgcgaac caagcggatg gcttgggcag gatcagccgt gagaaggatc atgcgaatgg
     9421 tcgtgcgtat gctcgtgaag aaagctatcc cccagggcat gcatggataa ggcggggacg
     9481 atccttccta ccagcacgcc tcgcgttctt acgatttcat cggccatttt ttcaagatca
     9541 cgggctggcc ccctcgccac gatgatttcg agacaatggc tgtggtccag atgaacatgc
     9601 atggtggaga tgacgtgtcc gccgctgcca tgttgaatgg atacgagttt gtccgtaagc
     9661 ccagggatat ggtggtcata caccatggtg atggcggcca cgacgtcttc gcctcggtcc
     9721 catgcttcgg aggcaagttc tacgcgcacc aagtcacgaa gggcttccga acgattggta
     9781 tagcctttgc gctgcacgta gtggtcaaaa cgttcgagca ggtcgtgagg catcgctacg
     9841 ccgaaacggg ccacgggctc acgagaggcg gaagcacgag aaggcatcgc ctaatctcct
     9901 cgaacggcag tttttttcgg gggttcgacc agaaacccca caatatgctc tcgctgttcg
     9961 aactcttttc gataacgtat cgcatcccag cgggacttga acggaccgat gcgcacgcga
    10021 taccaggttc cttttccagg caccaaagcg gattcgacat aggcgtgatg cccaccgcgt
    10081 cgcagtcggc tggcgaatgc ctccgcctct ttttcggttc gataggagct tacctggagt
    10141 tgatatccgc ctggttgccc cttcgcgacg gcctctcctc tgggattggc ggccgaagcg
    10201 gccatggcgg tcaacgaatc cctgggttcg gccacgatgg gactgggcga aacatacgct
    10261 tgagcgggga gcggcacgac gggaagcgaa tctcccgcag taggaggtac cgtgggcatg
    10321 ccaggaggca agacgaaagg cggctgagcg gacgacgaac tcgctgcccg taacgcgatc
    10381 atggcggtcg tagggttcgg atcgtccgaa agcatcgtgg gaaagttcat ttgattccca
    10441 tcaagagccg gaacaccttg cccctgagag gacttgcctg ccagttccgc gaggggatct
    10501 acctgcggtt cattgccgaa ctgtggtcgt ttgacgaggg caaatacggc aaaaaccaga
    10561 caggctccag cgatggaagc gagcaccaac gctccgacgc gtgacccgcc tcgccgcgcg
    10621 tcgccctcct gaatttggtc cagattacga agacttccgt gctccatggg agctcctcct
    10681 cacatcgtcc gacataggac ccaaccatgg gttttcgcct cgtggatccc atcgatggct
    10741 gggcgtggat tcaggcaagg acgctgccga cacatctgct ggcaacaacc cgcagtggat
    10801 caacgcctcc accgcacgcg aaatgatggg gtttggtcga aggtcggcat tcatgcgctt
    10861 gtgggttgtc tacctgtttg ctcgaaccat gggcaaattt gcggcagaca acgttgagag
    10921 gatcgaggaa aaggcgggga gtcagccgga cgatacaagt gtaactatat gaaatacttt
    10981 ataatttcac agtaccgacg acaggttcga ggctacgatc gcacgtgttt tcgcgcggcg
    11041 cggcagggct ccccctcacg gacaggaagg ttcggcatcc acggtgcaaa cgccattttg
    11101 acagcttgcg aagtacaaac agggattggc gctgctcgct tcgcaggggg aaccatcatc
    11161 gagcttggcc acgcatcgat gggtggtgga atcgcaatag ctcgttccct tgcaaggaat
    11221 gaaggtattg ccttggatac cgcaggtgcc tcctacatcg gccatgggcg tgcccgccac
    11281 gcataccttg ccgctgcacc gcagcccgag caatccattg caagaaccgg tcgaagagtc
    11341 acaaggatcg cccaatccgc ctggttgctc gcatttgcct tggcgacagc gaaggtttcc
    11401 ccgacatatc acggtgtccc cacagctttc gccttgtccc gcgctttgtt tacagagtgc
    11461 tggatcagcg aaagacgtct catggcaaat caaatccgga gcacagccat cgttggcaaa
    11521 ttcgcactga cctccgacgc cgataggcgg ggcgcaggaa ccacaagggc ccaaccgatc
    11581 acaaaacccg ctcgtacact gtaaatcgct gaaacaactg gccccttccg tcagggtacc
    11641 caccaccatc ggacaggctg tcgttcgtcc ccctgcatac agttcgggac acgtcatgtt
    11701 gctcatgttc atcgcgcagg aaaccacgtc tgccacgttt ttttgtacgc ccggtccatc
    11761 gggaagcacc accgagcgac cgccagccac gtaacgagcc acgcacgtgg tttcatcgcc
    11821 atacagataa ctcaccatgt acggcgcaca gcgctgcatc aggtcacaga acgcttccgc
    11881 gtagctttcc aacgccatcg cctcggtcat ggcatcggga gtcgaatctt ccgaaccgtc
    11941 gggcaacaga acctcgtcgt tcgtgtccac ggccgtatcc gccggtccat cgaccgggtt
    12001 cgaatcgatg ctcgtgtctt cttgcccgac ttcggatacc gcatcggtcc ccgtttccac
    12061 cgatacgcaa ctatctgtcc cggcatcgta gccgtcgcga ggggaggagt cctcgttatc
    12121 cgatccgcag gaaaaaagaa gagccaaagg caaagcaatc cacaatcgac ctgcacccat
    12181 gagcccttca tagcatccgg cagccatccg agaagccata aatggtaccg ccccatccag
    12241 tagcatcgaa aggatgccgg gatacaacac gaccgggcga ttaacgaccc gcaataggcg
    12301 caaaaatatg gtagatgttg aggtggactg gattcgtctc ggtcgtgagg gaagcgccat
    12361 gaagcccagg caacaacggt ttgtggatct atctatcctg gctaccaggg gagtttacgc
    12421 gtggacgatt gtgatgctgc tcctagccat ggcattgatt ggttgcggag cggatacctc
    12481 cgacagggaa ggccctccgg ggacaggcgg agcctatggt tcaggaggac acacgagcga
    12541 cagtggttcg gacaccggca tctgtccacc gggaacgttt ggtcctactt gccagccgtg
    12601 tgttgcgggg cagtattgtg cagggggcga ggcggctccc gtggattgtg ggtcaggcat
    12661 ctac
//
LOCUS       DAOSOR010000101        12588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_4995_length_12588_cov_4.562196, whole genome shotgun sequence.
ACCESSION   DAOSOR010000101 DAOSOR010000000
VERSION     DAOSOR010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 12588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12588
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4995_length_12588_cov_4.562196"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="PK710_07655"
     CDS             complement(<1..641)
                     /locus_tag="PK710_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOT09628.1"
                     /translation="MKPRHSSRTRRALGHLEGPVVTQGREDIAQRLATIMGGDMDESM
                     ARAHVHGFHAYPARMHPTLARRAIELLCPKHGVVLDPFCGSGTVLVESRLAARHGFGT
                     DLNPLAVLLARLKSRSASAQQLQKLLSTAEKTVRHADSRRREKRGATQRYGTEDVQCF
                     EPHVLLELDGLRDGIKQVHDEFVADALRLVLSSILVKVSKQSADTSSVLVPRRL"
     gene            complement(638..1402)
                     /locus_tag="PK710_07660"
     CDS             complement(638..1402)
                     /locus_tag="PK710_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09629.1"
                     /translation="MTSDSQKQPPSCTVCGASIDPQEEQCPSCGATRDKRHTCPFCGV
                     VAVPTAHPEFRLACPSCGGPRIPMPSGTKLNKASLAALQASRRAYSSRAAWRVASGLT
                     AAFGLLAVMLLIGVFFIAKLSFFPLLMASIIAAAPLVFAALAFRKSKQTQSLIQSSLD
                     RAWLESTKQLAAHQGPIQAETLMQAYGIDADQASSLLVRLGANEEVVTQITDDGQLAL
                     SVHPPFRVAVPDVQKEPEQVEVLADSQQESANKASW"
     gene            1669..2910
                     /locus_tag="PK710_07665"
     CDS             1669..2910
                     /locus_tag="PK710_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09630.1"
                     /translation="MIFASVAGWLASCATGSPEDTAKPLPPDGGFSGADGDVSDGSQG
                     GSGGGGVGGTGGSGGVSGTGGTGGAPPTPGSQCETCSDEVPCKDGFTCVTSPAGHPFC
                     AADCDGAACPAGDYECVDLADYDAEATGKGCVPPGGESCPCTSALEGKTRPCFSTNSL
                     GTCPGDEKCISGKWGGCNAPTPEKEICDGKDNNCNGFVDMDEPGLTGNDLCAGGAAPP
                     HSGFACVNGKCELSGCEDGWAKYPPTLPVTAGCACKVDAADIDSASNNQCTAATDKGE
                     LPDEGSTPVVLEGTLHSDTDEDWYAIRTVDKNQVPNFNTYRVNIEFMPPNGNPGNEFL
                     FDVIRGTDGDPCTAAKTGLTSYDWCADSSSSPADDDQSAPYRVRVYRNPTATSTCSNY
                     KIRVSNGGAGACPAADACGTP"
     gene            complement(3217..4365)
                     /locus_tag="PK710_07670"
     CDS             complement(3217..4365)
                     /locus_tag="PK710_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOT09631.1"
                     /translation="MHLEFTETQSLIQQTARDYAQRRIVPVAAQIDRESRFPKDILKE
                     LAELGLMGVNIPASLGGAEAGAVAYVLAMIEISRACASTAVAMSVTNMVGEVITNFGT
                     EEQRNRYVPMLTSGSVVAGSFALSEGGAGSDPGAMRTTAHRDGNHWVLNGEKMWISTG
                     DHAGVFVVWARTADRQTAPGTRGISCFLVEPGTPGFSVGKHEEKMGLRGSTTVSLSFE
                     DCRIPASALLGEENRGFRIAMMALDGGRLGIASQAIGIASAALEETVAYVKQRKQFDR
                     PIADFQAIQWQLADSRTELDAARMLTFRAAWLKENKKPFSQEASMAKLFASETAVRVC
                     NRCVQMHGGYGFTKDYAVERHLRDCRVTTIYEGTSEIQRTVISRSLLR"
     gene            complement(4420..5721)
                     /locus_tag="PK710_07675"
     CDS             complement(4420..5721)
                     /locus_tag="PK710_07675"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HOT09632.1"
                     /translation="MHASSWLRLTGLSALAVLVACSVSNNDNNQYNNRSVGNPGGTQD
                     AAPPPANSTDADLQEEAPTWDTSANTDPDASGTSSCNTDPDEDFDNDGYSVNQGDCND
                     CDPNANPGAFDIIGGVDGGVGVDEDCDGIVDNEVIDCDQGFDIDDKDAMNAAKSLGLC
                     RTTTANAQGKDKTWGVISAKYVKADGSNGMDPLSHGIVPRFGAAGVQEGMSMVVLSSG
                     TARAPGQKGFESPEDAQMGTSSNPPPGYPKDSTSCDNVFTGDEDCNDPAALELEIRVP
                     TNAHSFSFNFNFYTYEYPSFICSRYNDFFVTLLDPKPKNLPDGNISFDQDGNPISVNN
                     SLLQVCKPQTAGGKAFPCPLGTTLLGGTGFDEPARNGPHAATGWLQTKAPVEPGTIIT
                     LRFAIWDTADHILDSTVLIDNFQWSVEPATAAETKPIDTPK"
     gene            complement(5763..7064)
                     /locus_tag="PK710_07680"
     CDS             complement(5763..7064)
                     /locus_tag="PK710_07680"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HOT09633.1"
                     /translation="MMKARWLVIPSALACGMILTIAACSATSDAPFGKGQNGSGGTAG
                     QSNQGGSGGMFLPGGGSGGGNSEVNPPCSATDPNADLDGDGYTIADGDCNDCSAKMNP
                     GAYDFFGNNIDEDCNGIADDEPFGCDENLPLDSDDPLQAARALGLCRFTSATATGAQK
                     TWGVISAAYVFADGSVSSGHPDDPFECVGNGGSGAPPNVLSHGLLPKFGPITPRQGST
                     MLALSSGIAREGVVGPSPAEAIMCTKSATPAGFPTPSSAACPGQYIDDTPIANDPMAL
                     ELRIRVPTNAKSLSFDFDFYTYEYPGYICTEYNDFFVALLYSQHPNTPPNHNISFDKP
                     GNPVSVNNGFLEVCKPGTHGKKTFQCPLGTAELMGTGFEDHAATGWLQTKTAVVPGEE
                     ITLRFAIWDMGDEILDSTVLIDNVAWDVEEGEIGTVRPPPK"
     gene            complement(7265..8437)
                     /gene="metK"
                     /locus_tag="PK710_07685"
     CDS             complement(7265..8437)
                     /gene="metK"
                     /locus_tag="PK710_07685"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOT09634.1"
                     /translation="MPNRHFTSESVTEGHPDKICDQISDAILDDILRQDPKARVACET
                     LTKTGMVVVAGEITTSAWVDMPKVVRETVKEIGYRSSGMGFDWETCAVLTAIEQQSPD
                     IAMGVNATAEKDQGAGDQGLMFGFACDETPELMPAPISFAHKLARRLSEVRKSNVLDF
                     LRPDGKSQVTLEYDGNRVARIEAVVISSQHSPDVSQSQIIEGIRTEVIDKVLPANLMD
                     KQTKIHINPTGRFVIGGPMGDSGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAC
                     YFARFLAKNVVASKLARRCEVQLAYAIGVAQPVGVYVDTFGTGSVDDDKLAAYLAEHF
                     DMRPRAIIERLDLLRPIYKPTAAYGHFGRDGFSWEDTSPAAKIADDFARPTAKPVK"
     gene            8732..10504
                     /locus_tag="PK710_07690"
     CDS             8732..10504
                     /locus_tag="PK710_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOT09635.1"
                     /translation="MPILDPHSFSDTRQGRIRHIDFDFEVDFETQTLRGIGHYTLEAP
                     AAGSLFFDTRGIFIERVSSGNTELEWEIDEQDRVRGERLHIKNSRAVSSLIIVFRTSP
                     QATALGWLAPSQTAGGKYPFLLSQCQAIHARSLIPCQDTPSVRFTYTAKVKVMAPLTA
                     AMSADRTKVESQGAFNLCHFEMPQAIPSYLFALAVGNLAFQRIGKRCGVFSEPEILEQ
                     AAWEFADTERFIEEAEKLFGPYLWETYNMLILPPSFPYGGMENPRLTFLTPTLIVGDR
                     SMVNVVAHELAHSWTGNLVTNATWEDFWLNEGWTVYAERRLLEAIEGEPYAQLHAAVR
                     RKSMLEDMRVFGFESDATKLHFSQKGIDPDEVFSTIPYEKGCSFLSVIENVVGRDRFD
                     VFIRKYIETFRFQSLTTQDFLSFLREEIPGIDEKVNIQQWVFEPGFPRDALEPSSHLL
                     DDVYQRLKAYEQGVLPKMEDVSGWKAPQIEVFLKGMPRPIPVEHCAYLGELFGLDKSR
                     NCNLQTDYYVIAIVSGYTPIRKDVERLLSSVGRMLYLKPLYRALVDSAWSREFVRPLF
                     ETCRHSYHPIAVTAIVRILESAGL"
     gene            10712..12580
                     /locus_tag="PK710_07695"
     CDS             10712..12580
                     /locus_tag="PK710_07695"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT09636.1"
                     /translation="MSKASRICASCGSEYGQDALFCPKDGTPLGNSRTVPGEDPYLGL
                     VIGEQVELRQLIGIGSMGRVYRAFQRGVERDVAVKVLHRELSANLTLVTRFLREAKIS
                     SRLTHPNVVQVLMTGQLGTASNAAAVGGELYIVMEYLDGISLMSALAAAGGVMPLARA
                     LHVALQICDAVGEAHAQGIVHRDIKPENVMLVRRGDDPDYVKVLDFGIARLDWGEESF
                     ATQAGLIFGTARYISPEGSQGKPVSPSADVYAIATILYQCLSGRTPFDADSSVGLLMQ
                     HAYETPPALLDIPRASYVPPQLATVIMRNLSKSPEERCQDARIFGRELVEAARAGGLS
                     ADDLIPRSTLLGQRGVLTSFASIQRTKQMQLSAETAAAIHSSAAADSATDHESDRSKQ
                     NDTASEEPSLAAPSPGPPTRDNDDGSSWNEPMEAMRNDGDSIPTRSFDRRRIGVFIVF
                     CILAGAILTLVGAARLGRWGKLEESYVERANRAMSHQQWDAPPGQNVKDITDEGLVLH
                     PGDEALQQVRRRAADELIKSALGRKYAGDLEGALRHAKLARDFAPESTTAQHLVRELE
                     GLSMDAGVTTPEREPSGQGNETKKSVPPVGSSHRAIPLPSSQAASSSGAKAGRAPR"
BASE COUNT         2531 a         3109 c         4114 g         2834 t
ORIGIN      
        1 agtcgtcgag gtacgagcac cgacgatgta tcggccgatt gtttgctgac tttgacaagg
       61 atcgaggaga gcacgaggcg aagggcatcg gcgacgaatt cgtcgtggac ctgcttgatg
      121 ccgtcgcgca ggccatcgag ttcgagcagc acgtgaggtt cgaagcattg cacgtcttcg
      181 gtgccataac gttgcgtagc cccacgcttt tcccgtcgtc gtgaatccgc gtgtcggacg
      241 gttttttcgg cggtggacaa gagtttttgg agctgttggg cagaagccga tcgtgacttg
      301 aggcgagcga gaagcacggc gaggggattg aggtccgtgc cgaagccgtg tcgtgcagcg
      361 agtcgagatt cgacgagcac ggttccgctt ccgcagaacg ggtcgaggac cacgccgtgc
      421 tttgggcaaa gcaattcgat ggcgcgccgc gcgagcgtgg gatgcatgcg ggcggggtaa
      481 gcgtgaaacc cgtggacatg agcccgcgcc atggactcgt ccatgtcgcc gcccatgatg
      541 gtagcgagcc gttgggcgat atcctcacgg ccttgggtca ccacgggacc ttcgagatgt
      601 ccgagggctc gtcgtgttct ggaagaatga cgcggtttca ccaagatgcc ttgttcgctg
      661 attcttgttg tgagtccgca agcacctcga cttgttccgg ctctttctgg acgtcgggaa
      721 ccgcaacccg aaaaggaggg tgaacggaaa gcgcaagctg tccatcgtcg gtgatttggg
      781 taacgacctc ttcgttggct ccgagtctga caagaaggga agatgcctgg tcggcgtcga
      841 tgccataagc ctgcatgagt gtttcggctt ggatggggcc ctggtgggcg gcgagttgtt
      901 tggtggattc gagccaggcc cgatcgaggg aagactgtat aagcgattga gtttgtttac
      961 tttttctgaa ggccagggcg gcgaagacaa ggggagcggc ggcgatgata gaggccatga
     1021 gcagcgggaa aaaagacagc ttggcgatga aaaaaacgcc tatcagcaac atcaccgcca
     1081 gcagtccgaa agcggcggtg agtccactgg ccacgcgcca agcggcgcga gaggaatagg
     1141 cgcgtcggga cgcttgtagc gcggcgaggg aggccttgtt cagtttggtt ccggagggca
     1201 tgggaatacg tggaccgccg cacgaggggc atgcgagccg gaactccgga tgagccgtgg
     1261 gaacggccac cacaccgcag aacgggcatg tatgccgttt atcacgggtg gcgccgcacg
     1321 aggggcattg ctcttcctgt gggtctatcg atgcaccaca caccgtgcag gagggtggtt
     1381 gtttttgaga atctgaggtc atcggtgttc cttcgatgtt ctcgtagcgc tttggatgcg
     1441 ggaagccaac aggcggcaga atcaaagccc catgagcggc ggtgaccggt gttttccgat
     1501 gggtagagaa cgttcgaacg gtgatagtga cgcggatgtg tggtcgtttg ggccaatcgg
     1561 atttgaccga cggtatcgtt cgtgcgtaac cttatgaata ctgctcgtag taggaggtcc
     1621 gctttgattc tcgcgtcacc tttttgcgca cgccattgct ggacagtctt gatattcgcc
     1681 tcggttgcgg gttggcttgc ctcttgtgcg acaggatcac cggaagatac ggcgaaacct
     1741 ctgcctccgg atggtggttt ttcgggagcc gatggggatg tgagcgatgg ttcgcaaggg
     1801 ggatccggcg gtggcggggt ggggggaacg ggtggatctg gcggagtatc ggggactggg
     1861 ggaacgggag gcgcaccccc cacgccaggc tctcagtgcg agacatgctc ggatgaggtt
     1921 ccttgcaagg acggcttcac ttgcgtgaca tcgccagccg ggcatccgtt ttgtgcggcg
     1981 gattgtgatg gtgccgcgtg tcccgcgggc gattatgaat gcgtggattt ggccgactac
     2041 gatgcggagg ccactggcaa gggatgtgtc ccgccagggg gtgaatcatg tccatgcaca
     2101 tcggcgttgg aaggaaagac acgcccttgt ttctcgacca attcccttgg aacctgtccg
     2161 ggcgatgaga agtgcatctc gggcaagtgg ggtggatgca acgcgcctac gccggaaaaa
     2221 gagatttgcg acggcaaaga caacaactgc aacggatttg tggatatgga tgagcctggg
     2281 ctgacaggca atgacttatg tgctgggggt gccgcgccgc cgcactcggg attcgcttgt
     2341 gtgaatggta aatgcgaatt gtccggttgc gaagacggat gggcgaaata cccgccgact
     2401 cttccggtga ccgcgggttg tgcgtgcaag gtcgatgcgg ctgacatcga ttccgcttcg
     2461 aataaccaat gcacggcggc cacggacaaa ggcgagctgc cggacgaggg atcgacaccg
     2521 gtcgttctcg agggcacgct tcattcggat acggatgaag actggtacgc catccgtacc
     2581 gtcgacaaaa accaggtacc gaacttcaac acctatcgtg tcaacatcga gttcatgccg
     2641 cccaacggaa atcctggcaa tgagtttctt ttcgacgtga taaggggaac cgacggagat
     2701 ccatgcacgg cggcaaaaac agggctgacc agttatgatt ggtgtgccga tagttcatcg
     2761 tcgcccgcgg acgacgatca aagcgcaccg taccgtgtgc gtgtgtaccg caacccaacg
     2821 gccaccagta cgtgcagcaa ctataagata cgggtttcga atgggggagc tggagcgtgt
     2881 cctgcggccg acgcgtgtgg cacaccctaa tcggatagac gatcggatcc gcgtcctttc
     2941 gcttctttgg ccgtgaccgc agggggcgag gggagaaaaa tgggtagggc ggaaagctgg
     3001 ggtttctttt gtatcctgtt gaatttatga gctttttgaa caggcttcgg agcttggtgt
     3061 tcatgaggga gaggggaggg atggtgtatg gctttccctc gtaccaacgg gcagggattc
     3121 atcctcctgt tatcagtggg ggattgatcg gatacggata gatgctcgat ggctcgaggc
     3181 agggtcgaag gagggggcga agagattttt cgtgtactag cgcagcaaag accgtgagat
     3241 gacggtgcgt tggatctcac ttgttccttc gtagatggtg gtgacgcggc agtcgcgcag
     3301 gtgtcgttcg acggcgtaat ctttggtgaa tccgtagcct ccatgcattt gcacgcagcg
     3361 attgcagaca cggacggcgg tttcgctggc gaatagtttg gccatggagg cttcctggga
     3421 gaagggtttt ttgttttctt tgagccaggc cgcgcgaaag gtgagcatgc gagcggcatc
     3481 caattccgtg cgagagtcgg cgagttgcca ttggatggcc tggaagtccg cgatggggcg
     3541 atcgaattgt ttgcgttgtt tgacgtaggc gacggtttcc tcgagagcgg cggaagcgat
     3601 accgatggct tgggaagcga tgccgagacg tccgccgtcg agcgccatca tggcgatgcg
     3661 aaatccgcga ttttcttcac cgagcagggc cgaggcgggg atgcggcagt cttcgaagct
     3721 gagcgagacg gtggtagagc cgcgcaggcc cattttttcc tcatgttttc ccacggaaaa
     3781 gcctggagtg ccgggttcga cgaggaaaca cgagatgccg cgggtgcctg gggccgtttg
     3841 tcggtcggca gttcgagccc agacgacgaa gacgccggcg tggtcgcccg tggagatcca
     3901 cattttttca ccgttgagga cccaatggtt gccatctcga tgagcggtgg tgcgcatggc
     3961 accggggtca cttcccgcgc cgccttcgga aagggcgaag gaaccggcga caacgcttcc
     4021 cgaggtgagc atgggaacgt agcgatttcg ttgttcttcg gttccgaagt tggtgatgac
     4081 ttctcccacc atattggtga cggacatggc gacagcggtg gaagcgcaag cgcgagagat
     4141 ttcgatcatc gccaggacgt aagcgacggc gcctgcttct gcaccgccga gggaggcagg
     4201 gatattgaca cccatgaggc cgagttccgc tagttctttc aggatgtcct tgggaaatcg
     4261 ggattctcga tcgatctgcg cggcgaccgg gacaatgcgt cgctgtgcat aatctcgtgc
     4321 cgtttgttgg atgagtgatt gggtttccgt gaactcgaga tgcatgggct cggttgtagt
     4381 gcgggaggag cgatggctgc aaccgaggaa tcgcgctcgt tacttggggg tatctatggg
     4441 tttggtttca gcggccgtgg cgggttcgac cgaccattgg aagttgtcga tgagcacggt
     4501 cgagtccagg atgtggtcgg ctgtatccca gatggcgaag cgcaaggtaa tgatggtacc
     4561 gggctcgaca ggcgccttgg tttgtagcca gcccgtggca gcatgggggc cattgcgagc
     4621 gggttcatcg aaaccagttc cgccaagcaa cgtagttccc agcgggcatg ggaaagcctt
     4681 tcctcctgct gtctgcggtt tacatacctg caacagactg ttgttgacgc tgatggggtt
     4741 gccatcctga tcgaaagaga tattgccgtc ggggaggttt ttcggcttgg gatcgagcaa
     4801 ggtcacgaaa aagtcgttgt atcgactgca aatgaacgag gggtactcgt aggtgtagaa
     4861 gttgaagttg aacgagaagg agtgggcgtt ggtggggacg cggatttcga gttcgagagc
     4921 cgcagggtca ttgcaatctt cgtcaccggt gaaaacgtta tcacaggatg tactgtcttt
     4981 gggatagccc gggggcggat tgctcgacgt tcccatttgc gcgtcttctg gtgattcaaa
     5041 acctttttgc ccgggtgcgc gagcggtacc agaagaaagg accaccatgg acatgccttc
     5101 ctgcacgcca gcggcaccga aacgaggcac gatcccatgg gacaggggat ccattccgtt
     5161 cgagccatcg gccttgacgt acttggcgga gatgactccc caagttttgt ctttgccttg
     5221 cgcgttggcg gtggtggttc ggcatagtcc cagcgatttg gcggcgttca tggcgtcttt
     5281 gtcatcgatg tcgaagccct gatcgcaatc gatgacttca ttgtcgacga tgccgtcaca
     5341 atcctcatcg acacccacac cgccatcgac tcctccgatg atatcgaagg caccggggtt
     5401 ggcgttggga tcacaatcgt tgcaatcccc ttggttgacg gaataaccgt cgttgtcgaa
     5461 gtcttcatcg gggtccgtat tgcaagagga ggtgcctgaa gcatcggggt ccgtattggc
     5521 cgaggtatcc caggtggggg cttcttcttg taggtcggca tccgtcgagt tggcgggagg
     5581 gggcgcggca tcttgcgtac cgccggggtt gcccacactg cgattgttgt attggttgtt
     5641 gtcattgttc gagacagaac aagcgaccaa tacggcgaga gcgctcaagc cggtcaaacg
     5701 gagccaagac gatgcatgca tgaaaacctc ctgtcgaggc gatgttccga gcgaaagggg
     5761 gactacttgg gaggcggacg gacagtaccg atttcgcctt cttcgacgtc ccatgcgacg
     5821 ttgtcgataa ggacggtgga gtcaaggatt tcgtcgccca tatcccagat agcgaatcgt
     5881 agtgtgattt cttctcctgg gacgacggct gttttggttt ggagccagcc tgtagcggca
     5941 tggtcttcga agccggtgcc catgagttcg gcggttccga gcgggcattg gaaggttttt
     6001 ttgccgtggg ttcctggttt gcagacttcg agaaagccgt tgttgacgga gaccgggttc
     6061 cctggtttgt cgaaagatat attgtggttg ggtggggtat tggggtgctg gctgtagagc
     6121 agagccacaa aaaagtcgtt atattccgta cagatatagc cagggtactc gtaggtataa
     6181 aagtcgaaat cgaaggacag ggacttggcg ttggtgggaa cccggatgcg caattcgagc
     6241 gccatggggt cattcgcgat gggggtatcg tcgatgtatt gtcctgggca cgctgcggaa
     6301 gaaggtgtgg ggaagccggc gggagtagcg cttttggtac acatgatggc ttcggcgggg
     6361 gaaggtccga cgaccccttc gcgagcgatt cctgacgaca aggccagcat ggttgagcct
     6421 tgacggggcg tgataggccc gaacttgggg agcaagccgt gggacaagac attgggaggg
     6481 gcgccggagc cgccgtttcc aacacattcg aagggatcat cggggtgtcc ggaactcacc
     6541 gagccatcgg cgaatacgta ggcggcgctg atcactcccc acgttttttg tgctccggtt
     6601 gccgtggcgg aggtgaaacg gcacaagccg agagcgcgag cggcttgcaa cggatcatcg
     6661 ctgtccaagg gaagattctc atcgcatccg aaaggttcgt cgtcggcgat gccattacag
     6721 tcttcatcga tgttgttgcc gaaaaagtcg taggcccctg gattcatttt ggcggagcag
     6781 tcattgcaat ccccgtcggc aatcgtgtag ccgtcgccat cgaggtcggc gttggggtcg
     6841 gtagcggagc atggtggatt gacttcggag ttgccacccc cggagccgcc gccgggaaga
     6901 aacataccgc ccgagcctcc ttgattgctt tgtcccgcgg ttccgcccga accattctgt
     6961 cccttgccga aaggcgcatc actggtggcc gaacatgccg caatcgtcag gatcatccca
     7021 caggcaagag ctgaaggaat aacgagccaa cgagctttca tcatggggtc tccgatctga
     7081 accgccgacg taagtcagaa tgaaggcagc caatcgcaat acgttggaac aatactagaa
     7141 gatagcgtct cttgaagagc ttgtcacatg ggagtgtgcg agcagaggcc gtaacggatg
     7201 cgatgtcaag tgccgggtca tggcgccgcg ggccgtatcc gaagggccac gggggattgc
     7261 gggtctactt cacgggcttg gcggtggggc gagcgaagtc atcggcgatt tttgccgcgg
     7321 gcgatgtatc ctcccaggag aagccgtcac gtccgaagtg gccgtaggcg gcggtgggtt
     7381 tgtagatggg gcgcagcagg tcgagccgct cgatgatggc cctggggcgc atgtcgaagt
     7441 gctcggcaag atacgccgca agcttatcat cgtcgactga gcccgtgccg aaagtgtcga
     7501 cataaactcc cacgggttga gcaacgccga tggcataagc caattggact tcgcaccgac
     7561 gggcgagttt ggaagccacg acgtttttgg caagaaaacg tgcaaaataa catgcggagc
     7621 ggtcgacttt ggaggggtct ttcccgctga atgcgccacc accgtggcgt cccatgcccc
     7681 cgtaggtatc gacgatgatc tttcgtcctg tcaaaccgga gtcccccatg ggaccgccga
     7741 tcacgaatcg cccggtcggg ttgatatgga ttttggtttg tttgtccatg aggttggcgg
     7801 gaagcacctt gtcgataact tccgtgcgga ttccctcgat gatttggctc tgagatacgt
     7861 cggggctgtg ttgggaactg atgaccaccg cttcgatacg cgcgacgcga ttgccgtcgt
     7921 actcgagggt gacctggctt ttgccgtcgg ggcgaaggaa gtccaacaca ttggattttc
     7981 gcacttctga caagcgtcga gcgagtttat gggcgaagga gatcggcgcc ggcatcaatt
     8041 ccggagtttc atcgcaagca aagccgaaca tgaggccttg atctcccgca ccttggtctt
     8101 tttcggccgt ggcgttgacg cccatggcga tatcgggaga ttgttgttcg atggcggtga
     8161 gcacggcgca ggtttcccaa tcgaaaccca tgccggagct gcggtagccg atttccttga
     8221 cggtttcgcg aacgaccttg ggcatatcga cccaagcgga cgtggtgatt tctccggcaa
     8281 cgacgaccat acctgttttg gtgagggttt cgcaggcgac gcgggccttg ggatcctgtc
     8341 ggaggatgtc atcgaggatg gcgtcggaga tttggtcgca gattttatcg ggatgacctt
     8401 cggtcacgga ctcagatgtg aagtgtcggt tgggcatgac atacctatgg gtgcaatgtg
     8461 ctgcggaatt gaagtcgagc ccccaaggct cggcgaggac ttcctaaggc tcaacccccc
     8521 atgtcgtcaa ctgaagcttt ggaactgatg ggaggggtgg tgtcgttgcc atggattggc
     8581 gaaattggca ttggcaggcg cgattggcaa agcggcttgg tggcggcatt cgctggctca
     8641 cgatgtttca gcagttcgat aagcaatggg cagggtagga gtcagggttg cgaacgcttc
     8701 ttcttggcac tacggttcgg cttgatttct catgcctatc ctcgatcccc attctttttc
     8761 cgacacgcga caagggcgca tccgacatat cgacttcgat ttcgaggtgg atttcgagac
     8821 gcagacgctt cgcggcattg gacattacac tctggaagcg cctgcggcgg ggagcttgtt
     8881 tttcgatacg cggggtattt tcatcgagcg cgtgtcgtcg gggaacaccg agttggagtg
     8941 ggagatcgac gaacaagatc gtgttcgggg agaacgtctt cacatcaaga actctcgagc
     9001 cgtgtcttcg ttgatcatcg tgtttaggac atccccgcaa gccacggcgt tgggatggct
     9061 cgctcccagc cagacggcgg gaggtaagta tccgttttta ttgagtcaat gccaggcgat
     9121 ccacgcccga agtctgattc cttgtcagga tacgccgtcg gttcgtttca cctacacggc
     9181 gaaggtcaaa gtgatggcgc cgctgacggc ggcgatgtcc gcggaccgta cgaaggtgga
     9241 gtcccagggg gcgttcaatc tctgtcattt cgagatgccg caagcgattc cgtcgtacct
     9301 cttcgcgttg gccgtgggaa atcttgcctt ccagagaatc ggcaagcgat gtggtgtgtt
     9361 ttcggaaccc gagatcctcg agcaagcagc gtgggaattt gccgataccg aacggttcat
     9421 cgaggaagcc gagaagctat ttgggcctta tctttgggaa acctacaaca tgctgatcct
     9481 tcccccatct tttccgtatg gtggaatgga aaatccgagg cttacgtttc tgacgcccac
     9541 gttgattgtg ggggaccgtt cgatggtgaa tgtggtggcg catgagttgg cgcattcctg
     9601 gacgggcaac cttgtgacca acgccacgtg ggaagatttt tggttgaacg aaggatggac
     9661 ggtgtatgcg gagcggcgtt tgctcgaggc gatcgagggt gagccttacg cgcagcttca
     9721 tgcggcggtg cgtcgtaaaa gtatgttgga ggacatgcgt gtattcggtt tcgaatcgga
     9781 tgcgaccaag ctgcattttt cgcagaaagg gattgatccg gacgaggtat tttcgacgat
     9841 tccctatgaa aaagggtgtt cgtttttgtc ggtgatcgag aacgtggtgg gaagagatcg
     9901 tttcgacgtg ttcattcgca agtacatcga gacatttcgt tttcagtcgt tgaccacgca
     9961 ggacttcctt tcgttccttc gggaggaaat ccccggcatt gacgagaagg tgaacattca
    10021 gcagtgggtc ttcgagcctg gatttccccg tgatgccctc gagccctcct cgcatttgct
    10081 cgacgacgta taccagcggt tgaaggcgta cgagcaaggg gtattaccga agatggagga
    10141 tgtgtcgggg tggaaagctc cgcagatcga agtgtttttg aaagggatgc cgcggccgat
    10201 tcccgtggag cattgtgcgt acttgggtga gctttttggg ttggacaaga gccgtaattg
    10261 caatcttcag acggattatt atgtgattgc gattgtgagt ggttacacac cgatccgaaa
    10321 ggatgtggag cggttgcttt cgagcgtggg gcgaatgctg tacctcaaac cgttgtaccg
    10381 tgcgctggta gactcggcct ggtctcgtga attcgtcaga ccgttattcg aaacgtgtcg
    10441 tcattcctat catcccatcg cggtgacggc gatcgtacga atcctggaat cggcgggtct
    10501 ttgagccgtt gcggggcctt gcctgcccgg ttgtgcatgg tccatggttc aatcacaacg
    10561 cttgacgtag ccttgtgggg ctcgttttgg cctttcgaat cgaaaggtcg aggcagttgt
    10621 cggcccatga gtcgaggaag gcatgtcgat aggtcgaggt atgatcgaga catggaagcg
    10681 taatccagat cgctgctaga ctacccgcag gatgtccaaa gcctcgagga tctgcgcatc
    10741 gtgtggctcg gaatacgggc aagacgcgct attttgtccg aaggatggta cgcctttggg
    10801 caactctcgc acggtgcctg gtgaagatcc ttatttgggc ctggtgattg gggagcaagt
    10861 cgagttgcgc caactcatcg ggataggttc gatggggcgc gtataccgtg cgtttcagcg
    10921 gggcgtagag cgggatgtgg cagtgaaagt gctgcaccgg gagctgtccg cgaaccttac
    10981 tctcgtcacg aggtttctcc gggaagcgaa aatctctagc cgtctgactc accccaacgt
    11041 ggtgcaggtc cttatgacgg gccagctagg taccgcatcg aatgcagcgg ccgtgggagg
    11101 tgaactgtat atcgtcatgg aatacctcga tggtatttca ttgatgtcgg cccttgcggc
    11161 agccggtggt gtgatgccgc ttgcccgtgc gttgcatgtg gcgctgcaga tttgtgatgc
    11221 ggtgggagaa gctcacgcac aaggcatcgt gcatcgagac ataaaaccag aaaacgtcat
    11281 gttggttcgt cgcggagatg atccggatta cgtcaaagta cttgattttg ggatcgcgcg
    11341 gctggattgg ggagaggaat cgtttgccac acaggctgga ttgatttttg gcacggcgcg
    11401 ttacatttct ccagaggggt cgcaaggaaa gcctgtgtcg ccctcagccg acgtgtatgc
    11461 gattgccact attctttatc aatgcctgtc ggggcgaacg ccatttgatg cggattcatc
    11521 cgtagggctt ttgatgcagc atgcgtatga aacgcctcct gcgctgctgg atattcctcg
    11581 ggcaagctat gtaccaccgc aactcgctac ggtgatcatg cgaaacctgt cgaagtcacc
    11641 ggaagaacgt tgccaagatg cacggatttt cggacgcgaa ttggtagagg ccgcacgggc
    11701 gggcggtttg tctgccgatg acctcatccc tcgatcgacc ctgcttggtc agcgaggcgt
    11761 tctcacatcc tttgcgtcga tccaacgcac caaacagatg caactatccg ctgaaaccgc
    11821 cgctgccatc cattcgtcgg cagccgcgga cagcgcgaca gaccatgaaa gcgatcgttc
    11881 caagcagaac gataccgcaa gcgaggaacc gtcgcttgcg gctccttcgc ctggccctcc
    11941 gacacgtgac aacgatgatg gttcatcgtg gaacgaaccg atggaagcca tgagaaacga
    12001 tggagattcg atcccaacgc ggtctttcga ccgacggcgt atcggagtgt tcatcgtttt
    12061 ttgtattctt gcgggagcga ttctgacgtt ggtgggcgcc gcgcgtttgg gtcgatgggg
    12121 aaagctcgag gagtcttatg tggagcgagc gaatcgtgcg atgagccatc agcagtggga
    12181 tgcgccgcca gggcagaacg tcaaggacat cacggacgaa ggacttgttc tccaccccgg
    12241 agatgaagcc ctccaacaag tgcgtcgtcg tgccgcggac gaattgatca agtcagctct
    12301 tggacgtaag tacgctgggg atttggaagg tgcattgcgg catgcgaagc ttgcgaggga
    12361 ttttgctccg gaaagcacca cggcccaaca tctcgttcgc gaactcgaag ggcttagtat
    12421 ggatgcgggg gtgacaacac ccgaacggga gccatcgggc caagggaacg aaacgaaaaa
    12481 aagcgttcct cccgtgggtt cttctcatcg ggccattcct ttgcccagca gtcaggcggc
    12541 gtcttcttcc ggcgccaaag ccggccgcgc gccgaggtga tcaacgac
//
LOCUS       DAOSOR010000102        12487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5047_length_12487_cov_4.056950, whole genome shotgun sequence.
ACCESSION   DAOSOR010000102 DAOSOR010000000
VERSION     DAOSOR010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 12487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12487
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5047_length_12487_cov_4.056950"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="PK710_07700"
     CDS             complement(<1..1421)
                     /locus_tag="PK710_07700"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT09637.1"
                     /translation="MKGSSSPPPGSSTEPQGFGVEEPTSDAEISVTIDPDEIAAFSRE
                     AATATRVDEEIDTRAKRSVLVGSDRPPALESSAPEQGYGSSSRPSRFVDRYELIGLLG
                     VGGMGSVYRVRDLILQEEVALKILRKDLAEVASAVERFRSEVRLTRRITHPNVVRIHD
                     IGEHRGEHFYTMECIVGDPLSAAMGKGAAMDVVRAYEYGVQIAEGLAAVHQANVVHRD
                     LKPDNLMVSREGRVVITDFGVAVLRDGPSVDVLPLRGAGTPRYMAPEQLEGRSLTEKT
                     DLYALGLILFELLTGDIPWPKEDPNALDVARLSVPASSIHTINPRVPESLGRVVLALL
                     ERDPGGRPDSALALVEMLKQVRGEIGEQQVAGAEHGLQQQKQWPTIATTNTPHLRSVG
                     VMAFRNLGDPKDNYIAEALSMAIVDRLVVCPTVRVAKRFALRGPDNADVQFIGRQAGV
                     DIVVEGTLSKRASGAMAVTARVV"
     gene            complement(1540..3210)
                     /locus_tag="PK710_07705"
     CDS             complement(1540..3210)
                     /locus_tag="PK710_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09638.1"
                     /translation="MCRWLLVRLAAMLPVVLSWGCARGESVEQGRGDSGSDASTDGEQ
                     KEASESDGSSHVCDGVVCDDPPKNTCADAQHLTVYDTIGVCDEAGCHYAFHVEECLRG
                     CQNDGCVGEPCVGVKCDKPPDNECADEHHLTVFEVPGRCEEGQCIYDKHEQYCANGCE
                     GGGCQGDPCAGVSCLTPPANYCTDADHLEAFDGVGTCEGGVCGYGHHNEYCEHGCSAG
                     ACKGDPCAGVSCLTPPSNYCLTQDTLRRFSDSGQCSNGSCVYASTDIHCPFGCSQGAC
                     RECLGSTDCGAGRWCHDGNCEPCHTDQHCGASCVDCTTQIPAKKCDAVGSSCIECVSD
                     ADCGSGKWCDNHVCAVCNTAAHCGSQCVACSGATPECDGSACYCPSGTCVTCPTSALI
                     GAWDSGDDGWSWDGLWRRDKGYLLAGSTTKYSSNYTQNLTFGSDVDLSACGSASLSFM
                     IRLDDDPNFSPSSDKSERLYVQCSGDGGNQWQNLLPDSWPSKQSACATSYCSGGNGSS
                     RSFPWTSQRVTLPSSCRTATVRFRFQAKGANVWRLMDPGWWLDSVVVN"
     gene            3589..4593
                     /locus_tag="PK710_07710"
     CDS             3589..4593
                     /locus_tag="PK710_07710"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOT09639.1"
                     /translation="MRIAVTGGAGFIGSHLVERLLSDDHHVLCIDNFDPFYPAPVKLA
                     NLADVLHHPHFTLSSIDLCDANALSDSLAQFQPNTLFHLAATAGVRPSIERPMTYVRL
                     NVDATQTILSASLRHGVKRLILAGSSSVYGKDQPTPFREEHASDIPQSPYAATKRAME
                     LLAAAHAAVHPVTITVCRLFTVYGPRQRPDLAISRFVRAMLAHQPITVFGDGTFSRDF
                     TYVQDTVEGFLAAMHAPSGFHVYNLGAGKPFSVNELVSTIERVMQTQAIIRHAEEQKG
                     DVPTTFACTQRAEQQLGWSPRIDLEEGIRRFVAWMQSANPVYFQVGEPWSLGIPIDDS
                     "
     gene            4722..6617
                     /locus_tag="PK710_07715"
     CDS             4722..6617
                     /locus_tag="PK710_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOT09640.1"
                     /translation="MSLLQVTDIAFGYGAERLFRDVSFRLNAGERAALVAPNGAGKTT
                     LLRIIAGELTADDGTVVIRKEATVGFYRQSHELGTEGTVLDALLSSFQQVLAIRAELD
                     RARELAASGEPTDLTRLSRLEEQYQLAQGDELERRIQMIAQHLGFDNRALQRPVASLS
                     GGERGRLHLGIVLAQRPDLLMLDEPTNHLDLDTISWLESHLTEYPGAVLVVSHDRAFL
                     DNVCPHTLELGHKTFRSYPLTYTDYAIARKEILEREEALRERQQAMIDKTEEFIRKNI
                     AGQKTKQAQSRRRMLEKLEKLERPEDVWQVAQRVQFRFSPAPRSGDIVLQATGLGARR
                     GGIRLFHNLDLLVRRGERLAIVGPNGCGKTTLLNLLAGTGKPEDDGTVKVGANVFPGI
                     FDQHLGSLELSHSAIDEIRSVRGDMNEDVTRAYLSRFRFWGDQPFQKVASMSGGERSR
                     LALAKLLLEPRNVLFLDEPTNHLDIFAAEILEEALIHFEGTVVLVSHDRRFLENVSSR
                     VLAFRTDGIEVFPGGYGQLDVQTPVDPAASCPADTEDRAQRRAHFEANKAASRAQQRK
                     ERRFRELEELIDQAEKDLARIQRAMMEAGDDWEKLAKWTAQQQKIETDLAYFMKEWSE
                     LGDELTS"
     gene            6634..7368
                     /locus_tag="PK710_07720"
     CDS             6634..7368
                     /locus_tag="PK710_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HOT09641.1"
                     /translation="MNRGRWKWRFVGIALVSYGVASAGCSGCERNHDDGTRPVSQDLP
                     ALELRDDTPDVLLTWIDEKGDHHVVQKPADVPENARENVRVVTLQYGHGEMLYVADLR
                     TKNADGTYPVHSLPRSSWELIAQQRRSKAIAALTPFASQSAPTPAPAAPSSAQLSAIV
                     YMASWCSACRSAETYLRSQGVTVISKDIEKDPRAQKEMNEKLHKAGYPQKGTIPVLDI
                     RGKILVGFDQRAIKQAIQEAIRGDML"
     gene            7591..9237
                     /locus_tag="PK710_07725"
     CDS             7591..9237
                     /locus_tag="PK710_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09642.1"
                     /translation="MVTCSAWLIGCETPDGDSFAFNAPSKHDAGQEAADDTASSFPDS
                     CVPTTCEEQNAQCGTLPDGCGATIDCGECESGLVCGGAGPYRCGTKACIPKTCLQKDA
                     QCGWISDDCSQAIDCGGCMEPQTCGANHEPNVCGCSPKTCIQLGLQCGKALDGCGGTI
                     DCGTCAQGQHCGGGGPNLCGVGECQPKSCAQLGISCGLASDGCNSVVSCGDCEKPLVC
                     GGGGIPNECGCTPKTCAQLGASCGTIQTGCGEENCGSCTPPETCGGGGIENQCGCTCS
                     LPHASTHCSAGQCAILACDPGWDDCDGINDNGCETDLNQNPDHCGECENKCNISNGYA
                     ICNGGKCELDGCLDGFGDCDHKSENGCETTLATNPNHCGTCGRACSGPCVQGKCEYES
                     GEVARKWDQSYLGSGEWYTYDASKNYQGCYVGELGVLKSAIGAPDAMFSDLAGATVVE
                     ISVYLYAAHWYYFAGGTARIGVHGHASKPATFPGIDKDLKVDFERGEGKWIPLPSSWY
                     DSFKSGTYRGITLHANNSTDGQYYGYMTANATKWKWKYRK"
     gene            complement(9612..10985)
                     /locus_tag="PK710_07730"
     CDS             complement(9612..10985)
                     /locus_tag="PK710_07730"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOT09643.1"
                     /translation="MNDVSSHEPIVPSTPEGQVLQQKHGRKVARIAEQLRTRRSTAPL
                     SRQKRTVSHQVPKVHDKKHTDEKIDLLDLDEIIEVDVKRRICIAEPGVPFCKIVEKTL
                     PLGLVPIVVPELKTITVGGAVAGCSIESMSFQYGGFHDTCVEYEIITAQGDTLVCTPD
                     NEHSLVFQMMHGSFGTVGILSRLVFKLIEAKPYVHLRHEKHATIDSYLASIKRHFELQ
                     DVAFMDGIIHSNTQLSLCVGDFVDEAPYTNRYDWLKIYYKSTAERIEDYFETPYYFYR
                     YDQGVTNVHPKSFLGRLLIGKFLGSSEVLRIAEKARWLLLDDKRPDVTVDTFLPFSRL
                     KEFLQWYEKDCGHYPLWCVPYRRVHDYEWLTDEFWKGFGDELMVDIAVYGMRQRGDTN
                     YYRLLETKLQELNGVKTLISYNYYPEEEFWSIFHRKNFETVKAKTDPNNIFRNLYEKT
                     CQASMGR"
     gene            complement(11001..>12487)
                     /locus_tag="PK710_07735"
     CDS             complement(11001..>12487)
                     /locus_tag="PK710_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09644.1"
                     /translation="SHTADAGLDTRIEAFLDIVESYRRHVTPDDEKPFERRYSIAIKQ
                     EYCDVVDRKTGKRYDIRDPNVHLVWPSMGDLSTEAMDVVARRQGILSTHLPLPDVYST
                     QLARNVASGKECIPALLVLGSVLKFFQEHPPNQDDGIYVVFVPSTLGPCRTGQYHVFF
                     DRLFEEMGWENVVLLVANSENSYREMGATFNQDVWRALVLGDTFTDIRTSLRLLAKDP
                     IDAMVVFSAVWRDVMKALLAGPKQLDRELKRSAERLARIPRKAELQDLPKVLIVGEIY
                     VRRDNFSVDEVSEMLMAKGIYPKVTGVTEWFHYTDYARKFIMEGERKKKGWLRTLRDG
                     GVKDEAVYLVEKVWKEHVESKMLDVLRPTGLVPDVPHDMDEIIGKGREHFVDPEMESE
                     ATVSPAVGAAAMQEGYSGVAIIAPFGCLPGRLIEGVYAPWAKARGYPVLALENDGQPY
                     PPNIVSRIEVFAHHVKRFNATEPSPKKPSWRVLAEQVQRLTNWN"
BASE COUNT         2913 a         4268 c         2828 g         2478 t
ORIGIN      
        1 tccacgaccc gcgccgtcac cgccatcgct cccgacgctc gtttggatag cgtgccttcc
       61 acgacgatat cgacccctgc ctgccgcccg atgaactgca catccgcatt gtcaggcccc
      121 cgtaacgcaa aacgttttgc cacccgcacc gtaggacaga ccacgagcct gtccacaatc
      181 gccatcgaaa gcgcttccgc aatatagttg tctttcggat ccccgaggtt tcgaaaggcc
      241 atcactccaa cggaccgcaa atgcggcgtg ttggtggtcg cgatcgtcgg ccattgcttc
      301 tgctgctgta gcccatgctc cgcccctgcc acctgttgtt cgcctatctc cccccgcact
      361 tgcttcaaca tctcgacaag agcaagcgcg ctatccggac gccccccagg atccctctcg
      421 agcaacgcga gcaccactcg ccccaacgat tcgggaaccc gcggattgat cgtgtgaata
      481 gacgacgccg gcacggaaag acgcgccaca tccaaagcgt tcggatcttc tttcggccat
      541 ggaatgtcac ccgtcaacaa ttcgaacaaa atcaagccca gcgcatatag atcggtcttc
      601 tccgtcagag accgtccctc gagctgctca ggcgccatgt atcgaggcgt gcccgccccc
      661 cgaagcggaa gcacatccac cgaaggacca tcgcgcaaca ccgcaacgcc aaaatccgtg
      721 atcaccacac gcccctcgcg cgataccatc aagttatccg gcttcagatc acgatgaacc
      781 acgttggctt gatgaaccgc ggccaacccc tccgcaatct gcaccccata ctcatacgcg
      841 cgaaccacat ccatcgctgc ccccttgccc atggctgcgg aaagcgggtc gccaacgatg
      901 cattccatcg tataaaaatg ctcaccccga tgctccccaa tatcgtggat tcgaaccaca
      961 ttgggatgcg tgatccgtcg cgtgagacga acctcactgc gaaaacgttc caccgcggat
     1021 gctacctccg ccaaatcctt gcgaaggatc ttcaacgcca cctcttcctg cagaatcagg
     1081 tcccgaaccc gatacacgga acccatgccc ccaaccccca acaaaccaat gagttcatac
     1141 cgatccacaa atcgggatgg cctgctgctc gaaccgtacc cctgctccgg cgccgacgat
     1201 tccaacgccg gtggcctatc actccccacg agaaccgacc gcttggcacg tgtgtcgatt
     1261 tcttcatcca ccctcgtggc agtcgccgcc tccctcgaaa acgccgctat ttcatcaggg
     1321 tcgatcgtca ccgaaatctc cgcgtccgac gtcggctcct cgacaccaaa cccttgcggc
     1381 tccgtcgatg accccggagg tggcgatgac gaccctttca tacaataccc atcattcgac
     1441 tcgattctca ctccacactg gacccgcgtc accccaccaa cacaactgcc gctaccacac
     1501 catcctcatc gcttgccgtc catcctcttc ccttccgtat cagttcacca caactgaatc
     1561 aagccaccag cccggatcca tcaatcgcca cacattcgcc cccttcgcct gaaaccgaaa
     1621 ccgtaccgtc gccgtccgac atgatgacgg taacgtcaca cgttgtgacg tccacggaaa
     1681 cgaacgcgag gaaccattcc caccactaca ataactcgtg gcacaggctg attgcttcga
     1741 cggccaacta tcgggcaaaa gattctgcca ttgatttcca ccatccccag agcattgcac
     1801 atacagccgc tctgacttgt ccgaggacgg actaaaattc ggatcgtcat caagacgaat
     1861 catgaaactc aacgaggccg acccacacgc cgatagatcg acatcggagc cgaacgtgag
     1921 gttctgcgtg taattgctcg aatatttcgt ggtcgagcca gccagcagat agcccttgtc
     1981 acgcctccat aacccatccc aactccaacc atcgtcgccc gaatcccacg cgccaatcag
     2041 cgctgacgtc ggacaggtaa cacaggttcc ggacgggcaa taacaagccg aaccatcaca
     2101 ctcgggcgtc gctccagaac aggccacaca ctggcttccg caatgcgccg cggtgttgca
     2161 caccgcgcaa acatggttgt cacaccactt gccgcttcca caatccgcgt cactcacaca
     2221 ttcaatacaa ctcgatccca ctgcatcaca ttttttcgcc ggtatttgcg tggtgcaatc
     2281 cacacagctc gctccacaat gttgatccgt atggcacggc tcgcagtttc catcatggca
     2341 ccaccgcccc gctccacaat cggtcgaccc caggcattcg cgacaggctc cttgactaca
     2401 gccgaaggga caatggatat ccgtgctcgc gtagacacaa ctgccattcg aacactgccc
     2461 cgaatccgaa aaacgcctga gcgtatcttg cgtcaaacaa taattcgatg gcggcgttag
     2521 acaactcacc cccgcacatg gatccccctt acacgctccc gccgaacaac catgctcaca
     2581 atactcattg tgatggccgt atccacacac tcccccttca cacgtgccca ccccatcaaa
     2641 cgcctccaaa tgatccgcgt ccgtacaata attcgcggga ggcgtaagac aactcacccc
     2701 cgcacacgga tccccctggc accccccgcc ttcacaacca ttcgcacaat actgctcgtg
     2761 cttatcgtaa atgcattgcc cctcctcaca acgacccggt acttcgaata ccgtcaaatg
     2821 atgctcatcc gcacattcgt tgtcaggggg cttgtcacac ttaaccccaa cacaaggctc
     2881 acccacacac ccatcgttct gacaccctct cagacactcc tccacgtgga acgcataatg
     2941 acaccccgcc tcgtcacaaa ccccaatcgt atcgtatacc gtcaaatgct gcgcgtccgc
     3001 acacgtgttt ttcggcggat catcgcatac cactccatca caaacatgcg acgatccatc
     3061 cgactccgat gcctccttct gctccccatc cgtagaagca tccgacccac tatcccctcg
     3121 tccttgctcc accgattccc cccgtgcaca accccacgac aacacgacgg gcaacattgc
     3181 ggcaagccgt accaaaagcc atcgacacat ggtggggccc tccttcagaa atccattggc
     3241 tccctttgag tctatcacga tctgagccgt cccctcattt tccccaagcc tttcctgcac
     3301 ggtcacgatc acggtcacgg tcacggccca cgagaaacga tcacggtcat cccatgttgt
     3361 tcaccgcagt gcgccagccc catgtttagt agctcagggc aactgttggc ggtggcccaa
     3421 agggggtcaa ggatcgagcc aaaatcaaga tccagaaatt ctcagctcgc aactctttcc
     3481 cattcaggaa aaatgagagc ttctgacagt atcacgataa ctcccgttgc caacaccctc
     3541 cccttttccc gggttcccca tccgcctgca accgattatg cctcccctat gcgcatcgca
     3601 gtcaccggcg gcgccggttt catcggctca cacctcgtcg aacgcctcct ttccgacgac
     3661 catcacgtgt tgtgtatcga taacttcgat cccttctacc cagcgcccgt caagctcgcc
     3721 aacctcgccg acgtgctgca tcacccccac ttcaccttgt cgagcatcga cctttgcgat
     3781 gccaacgccc tatccgattc cctcgctcaa tttcaaccga ataccttgtt ccacctggcc
     3841 gccaccgcgg gcgtccgccc ctccatcgaa cgccccatga cctacgtccg gctcaatgtc
     3901 gacgctaccc aaaccattct ctccgcctcc ttacggcatg gcgtgaaacg attgattctc
     3961 gccggctcct ctagcgtata cggcaaagac caacccacgc cctttcgcga agaacacgcc
     4021 tccgatatcc cccaaagccc ctacgcggcc accaaacgcg ccatggaact actcgccgcc
     4081 gcccatgccg ccgtacaccc cgtcaccatc accgtttgca gactctttac cgtctacggc
     4141 ccaagacaac gccccgatct cgccatcagc cggttcgtgc gggccatgct cgcccatcaa
     4201 ccgatcaccg tctttggcga tggcaccttc tcccgagact tcacgtacgt gcaagatacc
     4261 gtcgaaggat tcctcgccgc catgcacgcc ccttccggct ttcacgtcta taacctcggc
     4321 gccggaaaac ccttctccgt caacgaattg gtctccacca tcgaacgtgt catgcaaacc
     4381 caggcaatca tccgccatgc agaagaacaa aaaggagatg tccccaccac cttcgcctgc
     4441 acacaacgag ccgaacaaca attgggttgg tcccctcgca tcgatctcga agaaggaatc
     4501 cgccgtttcg tcgcctggat gcaatccgcg aaccccgtct acttccaggt cggtgaaccc
     4561 tggtcactcg gaatccccat cgacgattcc taaattaatt caataatatc aagtagttat
     4621 ttcttcctga tcccatgcct ggccccttga ccgccgtcga ccccatcgga taccaacttg
     4681 cgaaaaggcg gtccaaacca gtatggacag acgattgcat catgtctttg ctccaagtca
     4741 cggatattgc gttcggatac ggggcggaac gcctgtttcg cgacgtatcc tttcgtctaa
     4801 acgccggtga gcgagccgcc ctcgtcgccc ccaatggcgc cgggaaaact acccttttac
     4861 ggataatcgc gggagagctg acggcggacg acggcacggt ggtgattcgc aaggaagcca
     4921 cggtggggtt ctatcggcag tcccatgaac tcggcaccga aggtacggtc ctcgatgccc
     4981 tgttgtccag cttccaacag gtgctcgcca tccgcgccga actcgaccgt gcccgtgaat
     5041 tggccgccag cggcgaaccc accgacctga ctcgactctc ccgactcgaa gaacaatacc
     5101 aattggccca gggcgatgag ttggagcgtc gaatccaaat gatcgcgcaa cacctcggct
     5161 tcgataaccg cgcgttgcaa cgccccgtgg cctccttgtc cggtggagag cgaggaagac
     5221 tgcacctcgg catcgttctg gctcaacggc ccgacttgct catgctcgac gaaccgacca
     5281 atcacctgga cctcgacacc atctcctggc tggaatccca tctcaccgag tatcccggcg
     5341 ctgttctcgt cgtatcccac gaccgcgctt ttctcgataa cgtctgtccc cataccctcg
     5401 aactcggtca caaaaccttc cgaagctatc cacttacgta taccgattac gccatcgctc
     5461 gcaaagaaat cctcgaacgg gaagaggcct tgcgcgaacg ccagcaggcc atgatcgaca
     5521 agaccgagga attcatccga aaaaacatcg ccggccaaaa aaccaaacaa gcccaaagcc
     5581 gtcgacgaat gctcgaaaaa ctcgaaaaac tcgagcgccc cgaagatgtc tggcaagtcg
     5641 cccaacgcgt ccaattccgc ttctcccccg cccctcgctc cggagatatc gtcctgcaag
     5701 ccaccggcct gggcgcccgc cgcggcggca tccgcttgtt ccataacctc gatttgctcg
     5761 tgcgacgagg cgagcgcctg gccatcgttg gccccaatgg ctgcggaaaa accacattgc
     5821 tcaacctgct ggccggcacc ggcaaaccag aagacgacgg cacggttaag gtgggcgcca
     5881 acgtctttcc cggaatattc gaccaacacc tcggctccct cgagctgagc catagcgcca
     5941 tcgacgaaat ccgctcggtc cggggggata tgaatgaaga cgtgacccga gcctacctgt
     6001 cgcgttttcg attctggggg gaccagccct tccaaaaagt ggcaagcatg tccggcggag
     6061 aacgtagccg cctcgccctc gccaaactcc tgctcgaacc acgcaatgtc ctttttctcg
     6121 acgaacccac caatcatctc gatatcttcg ccgccgaaat cctcgaagaa gccctgatcc
     6181 acttcgaagg taccgtcgtg ctcgtctccc acgaccggcg ctttctcgaa aacgtcagct
     6241 cacgggttct cgcctttcgc accgatggca tcgaggtctt ccccggcggc tacggccagc
     6301 tcgacgtgca gaccccagtg gatcctgccg cctcttgccc ggccgacacc gaggaccgcg
     6361 cgcagcgtcg cgcccacttc gaagccaaca aagccgcttc acgagcccaa cagcgcaaag
     6421 aacgacgctt tcgcgaactc gaagagttga tcgatcaggc tgaaaaagac ctcgcacgca
     6481 ttcagcgagc catgatggag gcaggcgacg attgggaaaa acttgccaag tggaccgctc
     6541 agcaacaaaa gatcgaaacg gacctcgcct acttcatgaa agaatggtcc gaactcggcg
     6601 acgaactcac ctcatgagca tgggagaacg tatttgaatc gtggacggtg gaaatggcgc
     6661 tttgtgggaa tcgccctcgt atcctacggc gtcgcaagcg cgggttgctc cggttgcgag
     6721 cgaaaccacg acgatggcac gcggcccgta tcgcaggact tgcccgccct cgaattgcgc
     6781 gacgatacgc cggatgtatt gcttacctgg atcgatgaaa aaggcgacca ccacgtcgtg
     6841 caaaaacccg ccgacgtccc cgaaaatgcc agggaaaacg tgcgggtcgt cacactccaa
     6901 tatggacacg gagaaatgct gtatgtagct gatcttcgta cgaaaaacgc cgatgggacc
     6961 tatccggtcc actccttgcc ccgcagcagt tgggaactca tcgcgcaaca acgtcgaagc
     7021 aaagccatcg ctgccctcac gccttttgcc tctcaatcag cgcccacacc tgcccccgcg
     7081 gccccttcct ccgcccaatt gtccgccatc gtctatatgg cttcctggtg tagcgcctgc
     7141 cgtagcgccg aaacctacct ccgttcccaa ggcgtcacgg tgatctccaa agacatcgaa
     7201 aaagacccgc gcgcacaaaa agaaatgaac gaaaaactac ataaagccgg atatccccaa
     7261 aaaggaacca ttcccgtcct cgatattcgc gggaaaatcc ttgtcggctt cgaccagcgc
     7321 gccatcaaac aagccatcca agaagcaatc cgcggcgata tgctctgatc cccgcccttt
     7381 cacagacacg gacctctcag atccccccta tgtgatccca ccgatcctat tattcgaacc
     7441 acggcttctt ttcacttacc actccgctag aatatagcga atatcgttcg tggtatatcg
     7501 tttgcttctc aacttttggt gggcattttg atccgttcaa ccaacaattc cgtccgtgat
     7561 aaaaatctgt cagcctcgta tctcctagcc ttggttacct gctcggcttg gctgattggc
     7621 tgtgaaacgc ctgacggtga ctccttcgcc ttcaatgccc ccagcaaaca tgatgccggt
     7681 caggaggccg ccgacgatac cgcctcttct ttcccggatt cctgtgttcc cactacctgc
     7741 gaggagcaaa acgcgcaatg tggcacgctg cctgatggct gtggcgcaac catcgattgc
     7801 ggcgaatgcg aatccggtct tgtctgcggc ggcgcgggcc cctaccgctg cggaacgaaa
     7861 gcttgtattc ccaaaacatg ccttcaaaaa gatgcgcaat gcggctggat atccgatgat
     7921 tgctcccaag ccatcgattg cggcgggtgc atggaacctc aaacctgcgg cgccaaccat
     7981 gagcccaacg tctgcggttg ttcccccaaa acttgcatcc agctcggcct gcaatgcgga
     8041 aaagccctcg acggttgcgg gggtactatc gattgcggta cgtgtgcgca aggacagcat
     8101 tgcggcggcg gcggtcccaa cctctgcggc gtaggcgaat gccaacccaa atcatgcgcc
     8161 caactcggca tctcctgcgg cctggcctcc gatggctgca attccgtcgt ttcttgtgga
     8221 gattgcgaaa aacccctggt ttgcggcggt ggcggcattc ccaacgaatg tggctgcacc
     8281 cccaaaacct gcgcccaact cggcgcttcc tgcggcacca tccaaaccgg atgcggagaa
     8341 gaaaactgcg ggtcctgcac tccccccgaa acctgcggcg gtggcggcat cgaaaaccaa
     8401 tgcggctgca cctgctcctt gccccacgcc tccacccact gctccgccgg ccaatgcgcc
     8461 atcctggctt gtgatccggg atgggatgat tgcgatggaa tcaacgataa cggttgcgaa
     8521 acagacctga atcaaaatcc cgatcattgt ggcgaatgcg aaaataaatg taatatttcc
     8581 aatggttacg caatatgcaa cgggggaaaa tgcgagctgg acggctgcct cgatggcttc
     8641 ggagactgcg atcacaaaag cgaaaacggc tgcgaaacca cccttgccac caatcccaac
     8701 cattgtggaa cctgcggacg cgcctgctcc ggaccttgcg tccaaggaaa atgcgaatat
     8761 gaatccggcg aagtagcccg aaaatgggac cagagctacc tcggctccgg agaatggtac
     8821 acctacgacg caagcaaaaa ctatcaagga tgctatgtgg gtgagctggg agtgctaaaa
     8881 tccgccatcg gcgctcccga cgccatgttc tccgaccttg ccggagctac cgtcgtcgaa
     8941 atctcggtct acctttatgc cgcccattgg tattacttcg ccggcggaac agcccgcatc
     9001 ggcgtccacg gccatgcttc caagcccgct acattccccg gtatcgacaa agacctcaaa
     9061 gtcgacttcg aacgcggaga aggcaaatgg atacctctgc cctcatcctg gtatgattcc
     9121 ttcaagtccg gcacctatcg cggaatcact ctgcatgcaa acaactccac cgacggccaa
     9181 tactacggct atatgaccgc caatgccacc aagtggaaat ggaagtaccg caaataagca
     9241 tgccgcgtac ctccacgggc cggccagcct gtcgagccct gagccggtca caccacacgc
     9301 aaatcggtta cggcccacgg cacacaaaat cggccacggc gcacggcaca caaaatcggt
     9361 cacggtcaca gtcacggtca cggcagaccc aaccgggcag caacgcaatc cggctggggt
     9421 ggaaatgctg agccattccc tcatttttcc ccgggccttt ccttcacggc cacggtcacg
     9481 gccaccgtca cggcgcacgg aaatcgctca cggtcacccc aatgaagtca aggaccaggt
     9541 caatagcagg atccagagga ttctccgctc gtatccctct cccatgcaag aacaaggagg
     9601 gaatctgtca gctaccgccc catcgacgcc tgacaggtct tctcatataa gttcctgaaa
     9661 atattgttcg gatccgtctt tgccttcacc gtctcgaagt tcttacggtg aaaaatgctc
     9721 caaaactcct cttccggata ataattgtac gaaatcaagg tcttgacccc gttcagctcc
     9781 tgcagcttcg tctcgagcaa tcgataataa ttcgtatccc ctcgctgccg catcccatac
     9841 accgcaatat ccaccatcaa ttcatcgccg aaccccttcc aaaactcatc cgtaagccat
     9901 tcataatcat gcacacgacg atagggcacg caccataacg gataatgccc gcaatccttc
     9961 tcataccact gcaaaaattc cttcaaacga gaaaagggca ggaaggtgtc taccgtcaca
    10021 tcgggacgtt tatcatcgag caaaagccac cgtgcctttt ccgcaatgcg aagaacctcc
    10081 gaagacccca ggaatttgcc gatcaacagc cgccccaaaa acgacttggg gtgtacgttc
    10141 gtcacgccct gatcataccg ataaaagtaa tacggtgtct cgaaataatc ctcgatgcgt
    10201 tccgccgtgc tcttataata aattttcagc caatcgtagc gattcgtata aggcgcctcg
    10261 tccacgaaat ccccgacaca aagcgaaagc tgcgtattgg aatgaatgat accgtccatg
    10321 aaagcaacgt cttgcagctc gaaatgacgc ttgatcgaag ccaaatagct atcgatcgtc
    10381 gcgtgtttct catggcgaag atgaacgtag ggcttggctt cgatgagctt gaataccaat
    10441 cgagacaaaa tgcccaccgt cccgaaagac ccatgcatca tttgaaaaac gagggaatgc
    10501 tcgttgtcgg gcgtgcaaac cagcgtatct ccttgcgccg tgatgatttc gtattcgaca
    10561 caggtatcgt ggaatccacc gtattgaaac gacatcgatt cgatggagca gcccgctacc
    10621 gccccgccca ccgtgatcgt tttcaactcc ggcaccacga tcggaaccaa ccccaacggc
    10681 agtgttttct cgacaatctt gcaaaacggt acccccggtt ccgcaatgca aatccgtcgc
    10741 ttcacatcca cttcgatgat ctcatccaaa tcgagaagat cgatcttctc atccgtatgc
    10801 ttcttatcat ggactttggg cacttgatgg gaaacggtcc ttttctgccg ggacaaggga
    10861 gccgtgcttc gacgggtccg tagttgctcc gcgatccgcg ccaccttgcg accatgtttc
    10921 tgctgaagca cctgcccctc gggcgtgctc ggaacaatcg gttcgtggct agacacgtcg
    10981 ttcatggatt cctcccacca tcaattccag ttcgtcaatc gctgcacttg ttccgccaac
    11041 acgcgccacg acggcttttt cggcgacggc tcggtcgcat tgaaacgctt gacatgatgc
    11101 gcaaacactt cgattcgcga tacgatgttg ggcggataag gctgaccgtc gttttccaac
    11161 gcaagcacgg gatagccccg cgccttcgcc cacggcgcat atacaccttc gatcaaccgt
    11221 cccggaaggc agccgaacgg cgctataatc gccacccccg aatagccctc ttgcatggcc
    11281 gccgccccca ccgcaggcga taccgtcgcc tcgctctcca tctccggatc cacgaaatgc
    11341 tcgcgccctt tgccaatgat ttcatccatg tcatgcggca cgtcgggaac caatcccgtc
    11401 ggacgaagca cgtcgagcat cttgctctcc acatgctcct tccacacctt ctccaccaaa
    11461 tacaccgcct cgtctttcac cccgccatcg cgcaacgtgc gcaaccatcc cttcttcttc
    11521 cgctccccct ccatgatgaa ctttcgcgcg taatccgtgt aatggaacca ctccgtcacc
    11581 cccgtcactt tcggataaat gcctttcgcc atcagcattt ccgaaacttc gtccacggag
    11641 aaattgtccc gacgcacgta aatctcgcct acaatcaata ctttaggaag atcttgcagt
    11701 tccgccttgc gcggaattcg agccaatcgc tccgcggagc gtttcaactc ccgatcgagc
    11761 tgcttcggcc ccgccaataa cgccttcatc acatcgcgcc ataccgccga aaacaccacc
    11821 atcgcatcga tgggatcctt cgccaatagc cgtaacgacg tgcgaatatc cgtaaacgta
    11881 tcccccaaaa cgagcgcgcg ccaaacgtcc tggttgaacg tcgcccccat ctctcgatag
    11941 gaattctccg aattcgccac caaaagcacc acgttctccc aacccatctc ctcgaacaac
    12001 cgatcgaaaa atacgtggta ctgccccgta cgacaaggac ctagcgtcga aggaacgaat
    12061 acaacgtaaa ttccgtcatc ctgattgggc ggatgctcct ggaaaaactt caataccgaa
    12121 ccgagcacca acaacgcggg aatacactcc tttccagacg ccacattgcg cgcaagctgc
    12181 gtcgaataca cgtccggcaa cggcaaatga gtcgacagaa tcccctgacg tcgagccacc
    12241 acatccatcg cttccgtcga taaatccccc atcgacggcc ataccaggtg gacgttcgga
    12301 tcgcgaatgt cgtagcgttt gcccgttttg cgatccacca catcacaata ctcctgtttg
    12361 atcgcaatgg aataccgtcg ctcaaagggt ttctcgtcat ccggggtgac gtggcggcga
    12421 tagctttcca cgatatcgag gaacgcttcg atccgggtgt cgaggcccgc gtccgcggta
    12481 tgagagt
//
LOCUS       DAOSOR010000103        12239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5175_length_12239_cov_4.798260, whole genome shotgun sequence.
ACCESSION   DAOSOR010000103 DAOSOR010000000
VERSION     DAOSOR010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 12239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12239
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5175_length_12239_cov_4.798260"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="PK710_07740"
     CDS             <1..833
                     /locus_tag="PK710_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09645.1"
                     /translation="TGIVLGTPYYMSPEQAQGDRQIDHRTDLWALGVITYEAMVGRRP
                     FEATNLGDLLVKICATDPPRPSSVYAGVPDGFDAWFSRACAHHPQDRFGTAKEMAVTL
                     SILCGTVPQGLSDSSYVNVFPSNSVKVDVARPDEPSGMIAENDPTQVMTEFPDSVRRV
                     MDQHAQENAKDSIVIKETNGESKKEAGLSSWSPIASASPVHVAAGILPQVDDEDDPVL
                     LFNSADIAADMERLKRVQQQQSARTRRQQLWNQYHWHVVSGLVLLVGILVALIVVFHK
                     "
     gene            complement(997..3198)
                     /locus_tag="PK710_07745"
     CDS             complement(997..3198)
                     /locus_tag="PK710_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09646.1"
                     /translation="MTRILLPITVGAALLAGAACGSTPRPQPTAASTPSIVATPVSND
                     HFGAALVQVLSDGSTSAERHALLAGVVRRQFARAEERFAAGQPDRALAAVKGALYLVR
                     VGELRLEMLDPSAAKALAGAHDAVAQRGIEGATLAFLRLRAQSLPATDPQQARIRAHI
                     EALEAWMKDTRQKSAVENASADARAFGELAVLEPSLEALEQARAMTDRWISASLDFNA
                     SFRPGMGRLNRDQMVEASRAFRAGAIIMAGLYLRHGDAKSATAALERTEARKITSPEL
                     FERLQTAASLNDPGAWRDLAALYSKAASEADEEELTVPPEIAEGATWGAALAAYRFAP
                     TQLETAGPLSMLLAAYGMPEAVPLVLAPVARDNPKPEILNTVLRVVAGVMMQEDESRD
                     YPSVQRVFAASHELLALADAVHSSTQLDPSPARLRSMMASLHVRNGALAAARPMFEQS
                     LREEPSLVGYSSLAAVLLQTGDTAGALEAVHQGLRAPNARELPTARAEAHLLAFQAHR
                     AQGARHEAQQDLAHALQDALDARAQARGDLGHASADRILARLAYHYGDLKAWQRAVDR
                     MLERVNLDARVLSMTLIEATSMGLLNHDLRTVHRVLDETIAAADPQDVVYASLWVLLA
                     ERAVGDNHDDAVKRALSAIDASHGWVYHLARYGLEEIGDDALRKKAKNLVERAEAEFY
                     IAMRKRVAGESTGDEELRNLAGGPAIDLVETHLARELTQPQTWGPPPKPLP"
     gene            3390..4874
                     /locus_tag="PK710_07750"
     CDS             3390..4874
                     /locus_tag="PK710_07750"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOT09647.1"
                     /translation="MPLEFRCATTDFSKESVDLLAVGVFAGEAVTTTYPVDTLDKAMG
                     GVLAEFIAQQEFTGKKDQTLEVDTLGRLAARRLLLVGLGEPKEISSAVYRTFAARAGR
                     NANGAKAKSLALVLPSKIQADDARALAEGVVLGAYRFTKYLTGDRLPKAELAHATVFT
                     SGKTDKLKSAMNLGARIAKSICITRDLVNEPPNELTPIVLASHAQQIAKQHGLDCKVF
                     DRKALERKGMKLLLAVGQGSVNEPRLVHLTYKPAKPSKKKLVWVGKGITFDSGGICIK
                     PAAGMGDMKSDMAGAANVIGLMACVAEIAPDVEVHGIIATAENMLDGNAYRPGDIFGS
                     LDGKTVEIINTDAEGRLVLADALAYARALNPSVLVCNATLTGACVVALGQHCSGFFTA
                     QEELAAKFNKAAADSGEQFWRLPLLEDMREQLKSDVADLKHTGDRWGGSISAALFLTE
                     FVGDTPFIHCDIAGPALAEKPYSFYSKGGTGHGVLAFLRFVETF"
     gene            complement(4918..5685)
                     /gene="sdhB"
                     /locus_tag="PK710_07755"
     CDS             complement(4918..5685)
                     /gene="sdhB"
                     /locus_tag="PK710_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HOT09648.1"
                     /translation="MSNPKVHLKIRRQDGIDQPNTRRWEEFEVPLLPHMNIISALQQI
                     QRNPVTKDGKRVAPVAWDCSCLEEICGACTMVINGKVRQACSALVQQLAPHGETIVLE
                     PMSKFPLVRDLIVDRTKMFEDLERVRAWIEIDGTHDLGAGPRESQEQQQLRYSLSRCM
                     TCGCCLEACPNVNDSSSFMGPAVVNQVRYFNMHPTGRHQRTARLESMMQRGGVAECGK
                     AQNCVEVCPKSIPLVDSLGHVARDTTKHAILGWLLGS"
     gene            complement(5694..7709)
                     /gene="sdhA"
                     /locus_tag="PK710_07760"
     CDS             complement(5694..7709)
                     /gene="sdhA"
                     /locus_tag="PK710_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HOT09649.1"
                     /translation="MSTNKHQQNASGPARRVIVVGGGLAGLMTTIKLCEARIAVDLFS
                     LVPAKRSHSVCAQGGINAAVNTMGEGDSPSIHLQETAYGGDFLANQPPIKGMTDAAPG
                     IVFLLDRMGVPFHRTPEGLLDFRRFGGSLYHRTAYAGATTGQQLLYALDEQVRRFATE
                     AVMDPTGMTLPGERLVRRFEGWDFLSLVLDDHGRCVGIVAQHLQDMVVRVFRAQAVCL
                     ATGGLGMVFGRSTNSMICTGSAAAIAFRQGAVYANGEFVQVHPTAIPGADKLRLISES
                     VRGEGGRVWVPKDAKDARPPSQIPHRERDYFLERRYPGYGNLVPRDIASRAIFQVCFH
                     EGRGIRNENTGKNEHEVYLDLTHIPPARLREKLGGVLEIYTKFVGADPCSQPMKVFPA
                     VHYSMGGLWVDYERNASGSLTVGSPRNHATSIPGLYAVGEVDYAYHGANRLGANSLLS
                     CLYGGLITGPAVASYVDALPSSAYDLPADFFEKAEENERKNVERTLARYGDNAEGINP
                     WKLHAQLSDVMLRDCTIERHDDRLDNALDTIETVREQLPKMRIEDTGERANQSAQFAR
                     HFENMVLLAHVIAKGARERSESRGAHYKPAFEKRDDAKWLRTTLAKYSSQEQVQFIRS
                     FDYDCVGQSIHVTDEVDVSLVPPRQRNYEKAGAASEQATGDAPAEAL"
     gene            complement(7721..8623)
                     /locus_tag="PK710_07765"
     CDS             complement(7721..8623)
                     /locus_tag="PK710_07765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09650.1"
                     /translation="MQASTLRIEEPRWTVTESAKAREPQSPWRNPKTRAFLLRKLFSL
                     TGVVPLAGFTIFHLAKNTSALQGREAFTAMAEQINGLPFVGLLEIVFILVPLMVHAAF
                     GILIVIDARYNVKAYGFNRNWMFTLQRVAGVLAIGFIGYHLYELRIQKMLGKLGVYGF
                     YDALSADLSSTIWGVPAVALIYVVGIAAVAFHLANGLWGFFCSWGITVSRRSQRLSAA
                     LLGIFGIVLFALGANITLYFATGSKLFVPSSSHSLGAADHCPPSALSSEPSSPESPSP
                     ELPSPELPSPASPSATPPPADSTP"
     gene            8801..10639
                     /locus_tag="PK710_07770"
     CDS             8801..10639
                     /locus_tag="PK710_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09651.1"
                     /translation="MSRTLVFNAVLWVAMGFVSCSRTSAEQPEPSLGRSVHAQTSFHV
                     IRERWLLSTWDERLALESSLEAFRQRHRTDPLTRVAEAYLALLALHRGDSRKALALAH
                     EVQQGLAGNTRDLSLLVQGAILARRGEPEAALQILDPLVGKLMDSFAQDLLHETVVNT
                     AVYAHRWFQAIVYMNEWVHAANEADAPTVRKRIEALLHGFPPAALDTALRAMSTRQKD
                     IWELALREALATHLARIAVTRSDPNLAKSLLETARTTVSEDELVPLARLATSAGGAPR
                     IIDARVGWLVETVSPAVSDRSAHALAGGLEVFKPQADAQNTAQTMSQAATGPPNLVFH
                     DVSSGDSRQDAMDSLVRDGVSIVVGGYDPAGASELAAYAESHSIAVVLLVAPVPPPQQ
                     PRFSFILGEADFSWSDLRASQAVSQVEVGKSARLGPGNPPELPYSYDCEGIPARVGDT
                     SFPVASWKSQKIEAVAIDGSAQCARRVLTDLQAIRYSPRVYLGLEAREAASAFSNDSI
                     LVASCGYFDSDLASIRAWRDFSGHDPTWFQALGRDAAWLARTAVEPLPRHTLTDVRDV
                     MKAREETQSRLAHAQGVLWTTKSSGFGGSRTMPRIIHWVSMSDKRR"
     gene            10663..11007
                     /locus_tag="PK710_07775"
     CDS             10663..11007
                     /locus_tag="PK710_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09652.1"
                     /translation="MLDSILIGIATILSALVVLLPWKVIPRFMQMANDFGETLPMGTR
                     FFLHGGFGLLSVLLTTTGTLLGCGLLVKGRRTMGRWLLIGATGVAMLSYVFVVLGLYL
                     PLVQTASALSAE"
     gene            11252..11548
                     /locus_tag="PK710_07780"
     CDS             11252..11548
                     /locus_tag="PK710_07780"
                     /inference="COORDINATES: protein motif:HMM:NF025384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HOT09653.1"
                     /translation="MQQRSPVTIVLLGFVTCGIYHLIWMHQVGEEMRARGAEVPPTWH
                     IIIPILNFLWMWKWAQAAEKVTNGALSAGLVFVLLWFLGPMAAFLVQPKFNEVH"
     gene            11677..>12239
                     /locus_tag="PK710_07785"
     CDS             11677..>12239
                     /locus_tag="PK710_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOT09654.1"
                     /translation="MKSASKLRLYNTLEQRLEDFSPIQPGKVGLYVCGPTVYDVPHAG
                     HARSAIAFDVLVRHLRARGLEVTFVRNITDIDDKILARARQNGEEPLQLSARMTQVYS
                     DDMGALHCLLPTHQPKVSDHLQEIIETVSTLIEQGAAYEVELPHGGRDVYFSVRAFEG
                     YGKLSRRKLEDLEAGARVEKNDLKRDP"
BASE COUNT         2556 a         3405 c         3682 g         2596 t
ORIGIN      
        1 gaacgggcat tgtcctaggg acgccgtact atatgagtcc cgagcaagcc cagggagacc
       61 gtcagatcga tcaccgtacg gacttatggg ctctgggtgt catcacgtac gaggccatgg
      121 tgggccgcag acccttcgaa gccacgaatc tcggtgactt attggtgaag atctgcgcca
      181 cggacccgcc tcgtccgtcc agcgtttacg ccggcgttcc cgatgggttc gacgcttggt
      241 tttcacgcgc ttgcgcgcat catccccagg accgtttcgg caccgccaaa gagatggccg
      301 tcacgttgtc gattctctgc ggaacagttc cccaaggcct ttccgattcg tcctatgtga
      361 atgtatttcc atcgaattcc gtgaaggtgg acgtcgcacg accggatgaa ccatcaggca
      421 tgattgccga aaacgatcct acccaggtca tgacggaatt cccagacagc gtccggcgcg
      481 tgatggatca acatgctcag gaaaacgcca aagactccat cgtcatcaag gaaacgaatg
      541 gcgaatccaa aaaagaagcc ggtttgagtt cttggtcccc catcgcttcg gcgagcccag
      601 tgcatgtggc cgctggcatc ctccctcaag tcgatgacga agacgatccc gtattgctat
      661 tcaattcggc agacatcgcc gcggacatgg aaaggctcaa acgtgtccaa caacagcaat
      721 ccgcccgaac gcgccgacaa caattgtgga accaatacca ctggcacgtc gtatcaggtt
      781 tggtgctgct ggtcggcatc ctcgtcgcac tgatcgtcgt gtttcacaaa tagggtgaaa
      841 ggcagactta gcggcttggg gaagcttgcc ttaggggtgg ctgtccgctc gccaccaagg
      901 gcgatgcgtc gactggggcc gagaggtatt tgtgaccacc gcgtgttgca attttaatca
      961 ataattttga caggttacaa ctaaaccctt cgatgatcag ggcagtggtt tgggcggggg
     1021 gccccacgtt tggggttgcg tcagttcacg cgcgaggtgc gtttcgacca ggtcgatcgc
     1081 ggggccaccg gcgaggtttc gaagctcctc atcgcccgtg gattcgccag caacgcgttt
     1141 tcgcatggcg atataaaact cggcttccgc gcgttccacg aggtttttcg cttttttccg
     1201 aagcgcgtca tcgccgattt cctctagtcc gtagcgtgcg aggtgataca cccacccatg
     1261 gctcgcgtcg atggcgctca aggcccgttt cacggcatcg tcgtgattgt ctcccacggc
     1321 tcgctccgca agcagaaccc acagggaggc gtaaacgaca tcttgggggt ctgcggcggc
     1381 gatggtttca tccagcacgc ggtgaacggt gcgcagatca tgattgagga ggcccatgga
     1441 agtagcttcg atgagcgtca tggacaagac acgagcatcg aggttgacgc gttcgagcat
     1501 gcgatcgacg gcgcgttgcc aagctttgag atcgccataa tggtaggcga ggcgagcgag
     1561 aatacggtca gcggaggcgt ggccgagatc cccacgcgcc tgggcgcgag cgtcgagggc
     1621 atcttgaagc gcatgggcga gatcttgttg tgcttcgtgc cgtgcccctt gggcgcgatg
     1681 agcttgaaaa gcaagcagat gagcttccgc ccgggctgtc ggcaactccc gagcattcgg
     1741 ggcgcggagc ccttggtgga cggcttcgag ggcgcctgcg gtatctccag tttggagcag
     1801 aacagccgca agggacgagt aacccacgag cgaaggttct tcgcgcagcg attgctcgaa
     1861 catgggtcga gccgcggcca aagcgccatt tctcacgtgt aaggaagcca tcatgctacg
     1921 aaggcgggcg ggggatggat cgagttgggt cgaggaatga accgcgtcgg ccaaggcaag
     1981 caattcgtgg gaagcggcga aaacacgttg taccgatgga taatcccggg attcatcctc
     2041 ttgcatcatc acgcctgcga cgacccgcag cacggtattg aggatctcgg gttttgggtt
     2101 atctcgcgcg acgggcgcaa gcacgagggg gacggcttct ggcataccgt aggcggcgag
     2161 gagcatggac aagggtcctg cggtttcgag ctgggtgggt gcgaagcggt aggcggccag
     2221 cgctgctccc caggtggcac cttccgcgat ttcggggggt acggtgagtt cttcttcatc
     2281 tgcttcggaa gctgctttgg aataaagtgc ggccaggtct cgccaggcgc cgggatcatt
     2341 caacgacgcg gcggtttgaa ggcgttcgaa taattcgggg gaggtgattt ttcgggcttc
     2401 ggtgcgctcg agagccgcgg tcgcactctt cgcatcgccg tgacgcagat acaatcctgc
     2461 catgatgatg gcgccggcgc gaaaggcgcg agaggcttcc accatctggt cacgattgag
     2521 tcgtcccatt ccgggtcgga aggaagcgtt gaaatccagg cttgcggaga tccaccgatc
     2581 ggtcatggcg cgcgcttgtt cgagcgcttc caaggaaggt tcgagaacgg ccagttcacc
     2641 gaaggcccgt gcatccgcgg aggcattttc cacggcgctt ttctgccgag tatccttcat
     2701 ccacgcttca agggcttcga tatgcgcacg gatacgcgcc tgctgcgggt ccgtcgcggg
     2761 aagggattga gcgcgaagac gcaaaaaagc gagagtcgcg ccttcgatcc cccgttgggc
     2821 caccgcatcg tgggcacctg ccaaagcctt ggccgcgctc ggatcgagca tctctaaacg
     2881 cagttctccg acgcgcacga gataaagggc tcctttgacg gccgcaagcg ctcgatccgg
     2941 ttgcccagcc gcaaagcgct cttcagctcg tgcaaattga cgtcgcacca ccccagccaa
     3001 caaggcatgc cgctccgcgg aagtgcttcc gtcagacaac acctggacga gggcagctcc
     3061 aaagtgatcg ttgcttaccg gcgttgccac gattgacggt gtggaggcgg cggtcggctg
     3121 tggacggggt gtcgagccac atgcggcccc tgccaacaaa gcggcaccaa cggttatggg
     3181 caacaagatt ctggtcacga tgcgcattgc catctcgttc gagtctatac ggcaatcagc
     3241 ccgagagagc acgtctcgag cgcgaaggat cgattttggc accgaatcgc taccaggacg
     3301 ggagtgccgc ttgatgaaag ggacaccatg atggcaagat gggccgccac cccgcagagg
     3361 cccagatcct ctgctcagtc ggaggaaata tgcccctcga gtttcgttgt gctacgaccg
     3421 atttttccaa agaatcggtg gatttgcttg cggttggtgt gtttgcggga gaagcggtca
     3481 ccacgaccta ccctgtggat accttggaca aagccatggg cggcgtgctg gccgaattca
     3541 ttgcgcagca ggaattcaca gggaaaaaag atcaaacctt ggaggtcgat accctaggcc
     3601 ggcttgccgc gcgacggctt ttgttggtgg gtctcgggga gcccaaggag atatcgagtg
     3661 cggtttatcg cacgtttgcc gcacgcgccg gacgcaatgc caatggcgcc aaagccaagt
     3721 ccctcgctct ggtgctgcca tcgaaaatcc aagctgacga tgcccgcgcg ctcgccgagg
     3781 gcgtcgtgct cggagcctat cgattcacca agtacctcac gggggaccgt ttacccaaag
     3841 ccgagttggc ccacgctacc gtattcacat ccggcaagac cgacaaactc aagtccgcca
     3901 tgaacttggg agcgcgcatc gccaagagca tctgcatcac gcgcgatctc gtcaacgagc
     3961 ctcccaatga attgacaccg atcgtcctgg cttctcatgc ccaacagatc gccaaacagc
     4021 acggactcga ttgcaaagtc ttcgatcgaa aagcccttga acgaaaggga atgaaactac
     4081 tattggccgt cggtcagggc agcgtgaacg agcctcgact ggttcatctc acctacaaac
     4141 cggccaagcc cagcaagaaa aaactcgtgt gggtgggcaa aggcatcacc ttcgattccg
     4201 gcggcatctg catcaaaccc gccgcaggaa tgggcgacat gaaaagcgat atggccggcg
     4261 ccgccaacgt catcgggctc atggcctgcg tggctgaaat cgcgcccgac gtcgaggtcc
     4321 atggcatcat cgcaaccgct gaaaatatgc tggatggcaa cgcttatcgc cccggagaca
     4381 tctttggttc tctcgatgga aagacggtgg agatcatcaa caccgatgcc gaggggcgtt
     4441 tggttcttgc agatgccttg gcctatgcac gcgcgttgaa tccctcggtg ctggtttgca
     4501 acgcgacgtt gacgggcgcg tgtgtggtgg cgttgggcca gcattgttcg ggatttttca
     4561 cggcccagga agaacttgcg gcgaagttca acaaggctgc tgcggattcc ggagaacaat
     4621 tttggcgcct tccccttctc gaagatatgc gcgagcagct caaaagcgat gtggccgatc
     4681 ttaaacacac cggcgatcgc tggggcggct ccatctccgc cgcactcttt ctcacggagt
     4741 tcgtgggaga tacccccttc attcactgcg atatcgcagg ccccgcgctg gccgaaaaac
     4801 cctatagctt ctattcaaaa gggggaacgg gccacggcgt gctggctttc cttcgcttcg
     4861 tcgaaacgtt ctaaccccta ccgaatcgca ataataatca tcaataaata cgaatagtta
     4921 cgaaccgagg agccatccca ggatcgcgtg tttcgtcgtg tctcgagcca cgtgtcccag
     4981 ggaatccacc agagggatcg acttcgggca tacttcgacg cagttttgtg ctttgccgca
     5041 ttcggccacg ccccctcgtt gcatcatgct ttcgagacgc gccgtacgct gatggcgccc
     5101 cgtaggatgc atgttgaagt aacgcacctg attgaccacc gccggtccca tgaacgaact
     5161 cgagtcattg acgttgggac aagcttccag gcagcagccg catgtcatgc agcgtgacag
     5221 agagtatcgc agttgttgtt gttcctggct ttcgcgaggc cccgcgccaa ggtcatgggt
     5281 accatcgatt tcgatccacg cccgaacgcg ctcgaggtct tcgaacattt tcgttcggtc
     5341 gacgatcaag tcccggacga gaggaaactt ggacatgggt tcgagcacga tggtttcacc
     5401 atgaggagcg agttgctgga ccaacgcgct gcatgcttgt cgcacttttc cgttgatcac
     5461 catcgtacaa gcgccgcaaa tttcctcgag gcaggagcaa tcccaggcaa cgggagccac
     5521 gcgttttccg tctttggtga ccgggtttcg ttggatttgt tgcagtgcgc tgatgatgtt
     5581 catgtgaggc aacaggggaa cttcgaattc ttcccaccgt ctggtattcg gttggtcgat
     5641 gccatcctgt cgccggattt tcagatgtac tttgggattg ctcatgaacg cccctacaac
     5701 gcctctgcag gcgcatctcc ggttgcctgt tcgctagcgg cgccagcctt ctcgtagttt
     5761 cgctgtcgtg gcggcaccaa gctcacatcc acctcatcgg tgacatgaat gctttggccc
     5821 acgcagtcgt agtcaaagga gcggatgaac tgcacctgtt cctgcgacga atatttggcc
     5881 aatgtcgtac gaagccactt tgcatcatcg cgtttttcga aagccggttt gtagtgtgcg
     5941 cctcgtgact ccgaacgctc acgcgcacct ttggcaatca catgggcgag cagcaccata
     6001 ttttcgaaat gacgcgcgaa ttgtgcgctt tggttggcac gctccccggt gtcttcgata
     6061 cgcattttcg gaagctgttc tcgtacggtt tcgatggtgt cgagagcatt gtccagacgg
     6121 tcatcatgac gctcgatggt gcaatcccgc agcatgacat ctgacaattg ggcgtgcagt
     6181 ttccagggat tgattccttc cgcgttatca ccgtagcgcg ctagggtccg ttccacgttt
     6241 tttcgctcat tttcctctgc tttttcgaag aagtcggcag gtagatcgta ggcgctggac
     6301 ggcaaggcat ccacatagct ggccaccgcg ggtccggtga tgagtccacc gtacaggcat
     6361 gagagaagcg agttcgcgcc gagccggttg gcgccgtggt aggcgtagtc gacctcaccg
     6421 acggcataga ggccgggaat gctggtggca tggttgcgcg gagagccaac ggtgagcgat
     6481 ccactcgcat tgcgttcgta atcgacccac agaccaccca tggaataatg gacggcggga
     6541 aagactttca tgggttggga acaagggtcc gcaccaacga atttcgtata aatttcaaga
     6601 actcccccta gtttttcacg gaggcgggct gggggaatat gagtgaggtc cagatatacc
     6661 tcgtgttcat tcttacctgt attttcgttt cgaataccgc ggccttcgtg aaagcaaacc
     6721 tggaagatgg ctcgggaagc gatatcgcgc ggaacgagat tgccatagcc agggtacctt
     6781 cgttcgagga agtaatcccg ctcgcggtgg gggatttggg aaggtggacg agcgtctttc
     6841 gcgtctttgg gcacccacac gcggccgcct tcgccgcgca cgctttccga gatgagccgg
     6901 agtttgtcag cgccgggaat ggcggtggga tgaacctgca caaactcgcc gttcgcatac
     6961 acggcgcctt gtcgaaaggc gatagccgcg gcacttcccg tgcaaatcat gctgttggtc
     7021 gaccggccga acaccatacc gagcccgccg gtggccaaac aaaccgcttg tgcgcgaaat
     7081 acgcgaacaa ccatgtcttg gagatgctgc gccacgatgc ccacacatcg cccatgatcg
     7141 tcgagcacca gcgaaagaaa gtcccaccct tcgaatctgc gaacgagccg ctcgcccggc
     7201 aacgtcatac ccgtaggatc catcaccgcc tcagtggcga agcgccgtac ttgttcatcg
     7261 agcgcataga gcagttgttg acccgtcgtc gcgcccgcgt aggcggtgcg gtgatacagc
     7321 gaaccgccaa accgacgaaa gtccaacaac ccttccggcg ttcgatgaaa cggcacgccc
     7381 atgcgatcga gcaagaaaac gatgccgggg gcagcatctg tcatgccttt gatgggaggc
     7441 tgattggcca aaaaatcgcc gccataagcc gtctcctgca gatggatcga tgggctatcc
     7501 ccttcgccca tggtgttgac cgcggcgttg attccgcctt gggcgcagac ggaatggctt
     7561 cgtttggcgg gtacgagaga gaatagatcg acggcgatac gcgcttcaca cagtttgatg
     7621 gtggtcatca aacccgcgag tccaccgccc acgacgatga cgcgacgagc gggacccgag
     7681 gcgttttgct ggtgcttgtt ggtgctcatg gtcgtttccc tcagggcgtg gaatcggcag
     7741 gtggcggtgt cgcggatggc gatgctgggg atggtaattc tggggatggt aattctgggg
     7801 atggtgattc tggggatgac ggctcggatg agagggccga aggcgggcaa tggtctgcag
     7861 cgccaaggga atgggacgaa gaaggaacga agagcttcga gcccgtggca aagtacaaag
     7921 tgatattggc accgagagca aacaagacga ttccgaaaat gcccaacaat gcggcggata
     7981 gccgctggga acgtcgcgac acggtgatac cccaggagca gaaaaagccc cacaacccgt
     8041 tggcgaggtg gaaagcaacg gcagcgatcc ccacaacgta gatgagagcg acggcaggta
     8101 cgccccaaat ggtgctggaa aggtcggccg acagcgcgtc ataaaagccg taaacaccta
     8161 attttccaag cattttttgt attcggagtt catacaagtg gtagccgatg aatccgatgg
     8221 cgagcacacc ggccacccgt tgcaaagtga acatccagtt ccgattgaac ccgtaagctt
     8281 tcacgttgta gcgggcatcg atgacgatca agatgccaaa ggcggcgtga accatcaggg
     8341 gaacgaggat gaaaacgatc tcgaggaggc cgacaaaggg caagccgttg atttgttcgg
     8401 ccatggcggt gaaagcttcc cggccttgca gagcggaagt gttttttgcc aggtgaaaga
     8461 tggtaaaccc cgccaggggc acgacacccg tgagggaaaa gagttttctc aggagaaacg
     8521 cgcgggtttt ggggttacgc cacgggcttt ggggttcgcg tgctttcgcc gattccgtca
     8581 ccgtccacct cggttcttcg attcgtagcg tcgacgcttg cattgtaacc tcgcggagtg
     8641 caagcgtttc gagccacgaa ggaccgtgaa ccccatcggt ggggcaaatc gtcatttcgg
     8701 ccgctcacgc gcaagcatct tgcttgcacg agggagccgc ggcggatggg cttgccttac
     8761 gctacgtttg cgcgccagac tcgttggcat cgtccgcgcc atgagccgaa ccttggtttt
     8821 caacgctgtc ctgtgggtag ccatgggttt tgtatcctgc tcccgcacgt ctgcagaaca
     8881 gccggaacca agccttggac gttcggtcca tgcgcaaact agcttccatg tcattcggga
     8941 acggtggctg ctgtcgacct gggacgaacg tctcgcgttg gaatcctcgc tcgaagcatt
     9001 tcggcagagg caccggacgg acccgctcac tcgtgttgcg gaagcgtact tggcactgct
     9061 ggcacttcac cggggtgact cgcgcaaagc cttggccctt gcccatgagg tccaacaggg
     9121 gctggcggga aacacgcgcg atctgagcct tttggtacaa ggcgccatcc ttgcgcgacg
     9181 aggcgaaccg gaagcagcgt tgcaaatcct cgacccgctc gtcggcaagc tcatggactc
     9241 cttcgcgcag gacctgctgc atgaaacagt agttaatacg gccgtttatg cccaccgctg
     9301 gtttcaagcg atcgtctata tgaacgaatg ggttcacgct gccaatgagg cggacgcgcc
     9361 tacggttcga aagcgaatcg aggcgttgct ccatggattt ccgccagcgg ctttggacac
     9421 ggcgctgcga gccatgagta cgcggcaaaa ggatatttgg gaactggcgt tgcgcgaagc
     9481 attagccacc catcttgcga gaattgccgt aacacgctct gatccgaacc tggcgaaaag
     9541 cctgctcgag acggcccgaa cgaccgtatc cgaagatgaa ttggtgccgc ttgcgcgact
     9601 ggcgacatcg gcgggcggtg cgccgcgtat catcgatgcg cgtgtgggat ggctcgtgga
     9661 gacggtatcg cctgcggtga gcgatcggtc ggctcatgcg ttggcgggtg ggctcgaggt
     9721 attcaagccg caagcggatg cgcagaacac ggcgcaaacc atgtcacaag cagcgacagg
     9781 tcctcccaat ttggtttttc acgatgtgag ttcgggcgat tcccggcaag acgcgatgga
     9841 tagtctggtt cgcgatgggg tttcgatcgt ggtggggggt tatgatcctg caggggcatc
     9901 ggaactcgcc gcgtatgccg aatcccattc gatagcggtg gttcttttgg tggctcccgt
     9961 cccgcctccc cagcagccgc ggttttcgtt cattttagga gaagccgatt tttcttggag
    10021 cgatttgcgc gcgtcgcagg ccgtatccca ggtcgaagtc ggcaagtcgg caaggcttgg
    10081 tcccggaaat ccgccggaac tcccttattc ctacgattgt gaaggaatcc cggcgcgcgt
    10141 gggagacaca tcgtttcccg ttgcgtcgtg gaaatcacaa aaaatcgaag ctgtcgccat
    10201 cgacggatcc gcgcaatgcg ctcgtcgagt tctcactgac ttgcaggcaa tccgttattc
    10261 tccacgagtt tatctggggc tcgaagcccg ggaagccgcc tcggcctttt cgaacgattc
    10321 cattttggta gcaagttgcg ggtacttcga tagcgacctg gcctcgatac gagcctggcg
    10381 ggacttttcc ggacatgatc cgacgtggtt tcaagccctt gggcgcgatg cggcctggct
    10441 cgcccgaacc gccgtggaac ctttgcctcg ccacacccta accgatgtac gtgacgtcat
    10501 gaaagcgcgc gaagagaccc aaagtcgttt ggcacatgcc caaggcgtgt tatggacgac
    10561 gaagtccagc ggatttggag ggagtcgcac gatgccacga atcattcatt gggtttccat
    10621 gtcggacaag cgtcgatgaa gcccacgccc acacgtgtga cgatgctgga ttctatcctg
    10681 attgggatag ctacgatctt gtccgcgttg gtggtgttgt tgccgtggaa ggtcattccc
    10741 cgattcatgc agatggcgaa cgactttggg gaaacgctgc ccatgggcac acgttttttt
    10801 ctccacggcg gattcgggct gctctccgtg ctcctgacca cgacagggac gcttctcggt
    10861 tgcgggcttt tggtgaaggg acgacgaacg atggggcggt ggctattgat aggggcgacc
    10921 ggcgtggcga tgttatccta tgttttcgtg gtgttggggt tgtacctccc cttggtccag
    10981 acggcatcgg ccttgtcggc ggaatgacgg attttcgtac tttttgcgag caaggattgg
    11041 ccgtcagtga tggaagagac aatcgacagg ggaagacatt ggatgaagta agcgtataca
    11101 atcgccgaaa tgggaacgtg tctggcccta tgatgaaacg gcaaaacaag ttccctgtcg
    11161 atttgcggtt tgtgatatgc gactcttcgg agtctcgatc ctcccatgca tcaccagctt
    11221 ggggctagaa aagagcgtaa ggagaacctc aatgcagcag cgtagtcctg tcaccatcgt
    11281 gttgctcgga tttgtcacgt gcggcattta tcatcttatc tggatgcatc aggtagggga
    11341 ggaaatgcgt gcgcgagggg cggaggtgcc gccgacgtgg cacatcatca tccccatttt
    11401 gaatttcttg tggatgtgga agtgggccca agcggctgaa aaggtgacca atggtgcctt
    11461 gagtgctggc ctcgttttcg tgctgttgtg gttcctgggg ccgatggcgg cgttcctcgt
    11521 ccagccgaaa ttcaacgaag tccactaact gccaacccct tctgtcctcg aaacacccca
    11581 agccggtcgt tcttggccgg tggtgccatc ttcttccccg caagcatcat cgacccactt
    11641 tccaagaccg tcgctcatgc tatatcggcc tatctcatga aaagcgccag caaactccgt
    11701 ctttacaaca ccctcgaaca acgactcgaa gacttttcac ccatacaacc aggaaaagtc
    11761 ggcctgtacg tctgcggccc cacggtctac gacgttcctc acgcgggtca tgcgcgatcg
    11821 gccatcgctt tcgacgtgct cgtgcgtcat cttcgcgcgc gcggtctcga ggtcacgttc
    11881 gttcgtaaca tcacggatat cgatgataaa atcctggccc gggcgcggca aaacggggaa
    11941 gagccactgc aattatccgc acggatgacg caggtttata gtgacgacat gggggcattg
    12001 cactgcttgt tgcctaccca tcaacccaag gtcagcgacc atttgcaaga gatcatcgag
    12061 acggtaagca cgttgatcga acagggtgcg gcgtatgagg tggagttgcc gcacggtggg
    12121 cgcgacgttt atttctcggt tcgagcgttc gagggttacg gaaagctatc gcgacgcaag
    12181 ctcgaggatt tggaagcggg agcgcgtgtg gaaaaaaacg atctcaaacg agatcccat
//
LOCUS       DAOSOR010000104        12173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5206_length_12173_cov_4.669005, whole genome shotgun sequence.
ACCESSION   DAOSOR010000104 DAOSOR010000000
VERSION     DAOSOR010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 12173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12173
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5206_length_12173_cov_4.669005"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PK710_07790"
     CDS             <1..284
                     /locus_tag="PK710_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09655.1"
                     /translation="TSTSELTPATVIHEPEAPQQAPPVETGEAAMGEPEDAETGENPD
                     GSQEEGPAAAASSASTAVPTPTTVPTRKPPIEVRVHPPKPVPPRRDGGY"
     gene            complement(327..851)
                     /locus_tag="PK710_07795"
     CDS             complement(327..851)
                     /locus_tag="PK710_07795"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOT09656.1"
                     /translation="MANERLLITPDVRVAQLLEVYPELEAVLVSLSPTFQALKNPVLR
                     RTVAKVATLRQVASVGGIGLGILINRLREAVGQEQQPTDAQPQQEPLPAPPWANPEQV
                     TASFDARPLLDAGGHPMQPVLSALNQLAPGQVYLLITPFVPAPLVDMAEKKGYATYSD
                     VRGDELVHTFFRRS"
     gene            complement(841..2094)
                     /locus_tag="PK710_07800"
     CDS             complement(841..2094)
                     /locus_tag="PK710_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HOT09657.1"
                     /translation="MSEVIDNATRRKELLKHMIRQLHDGTAPQAVRAQLARLLGEVPY
                     GDVVEVEQELMAEGLPEKEVLELCDVHAEALSGLVQIRKPKKPLPTGHPVHTFAAENQ
                     ALRLEVKQVAKLSADLTVADPQQDVSSVWSALRLRFNALMDVEKHYLRKEHLLFPFLE
                     KHGITGPPKIMWGKHDETRALLKSAAEALASTRTVTAGEAKGVIDLVLAPACKAITDM
                     TDKEEQILFPMCLDTLSSQEWFEIARQSSEIGFCLYDPPSEWRPDDLPESLAQESQSG
                     RIQLKSGSFDLAELEAMLNTIPFDITFVDAKDTVRYFTQGAERIFARTRAILGRKVQY
                     CHPPSSVHTVEQILEQFRNGNKDRAAFWIEMKGRFISIEYFAMRDADGQYLGCIEVSQ
                     DLTDKRALQGEQRLLTWEREVEHGE"
     gene            complement(2248..3000)
                     /locus_tag="PK710_07805"
     CDS             complement(2248..3000)
                     /locus_tag="PK710_07805"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOT09658.1"
                     /translation="MAGRTFAVGDLHGGLDQLFRLLSCFPELDAEDTLVFIGDYVDRG
                     PKSAQVVDYIRNLPSMTRANVVALRGNHEDAWLRVVDHGWDEFVMPPLNGCLAAFRSF
                     VDGPVPSENELPRADERMMIQTGAFFPDDVVQWFRQLPLWYEDEHAIYVHAGLPQHPD
                     GGFYHPRQVTDSMSLLWGRDEEFFSNYTGKLVVFGHTGTEYLAQEIPKGVMNDQIGIW
                     RCGDAIGIDTGCGHGGYLSALELPAMNIYDSR"
     gene            complement(3304..4413)
                     /locus_tag="PK710_07810"
     CDS             complement(3304..4413)
                     /locus_tag="PK710_07810"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOT09659.1"
                     /translation="MNRYLQIYRIILKTFGFRLRPIGTFALKQAHSVVNAATLAADHL
                     WSPSFTRQPIDRPVFILGNPRSGTTFLHRFLLHTDSLAAFELWEMLFPAITARRTIGP
                     FVDRLAPLSPARYHSAAAHETSLRDVETDDALALFRFVDGAFLWTYFLAWQDHWGSES
                     ARALFELDQDSAENIERHFHYMEGCWRRNLLVKNKSRIAVKASSYTLRIKTLLHRYPD
                     CKLLYVVRDPVETIPSGMSMITGVLEQSYDVFHSTREQDRKRYLDNLYQASCYLFRYF
                     HEVWKQGAIPEKNLLIVRYPQLMADLESTMRDIVDFLEIEPPTQFRTRVQQQAIVQRQ
                     RKSPHTYSLEKFGLTAEKIRSDLAFVIDDFDLACP"
     gene            complement(4415..5440)
                     /locus_tag="PK710_07815"
     CDS             complement(4415..5440)
                     /locus_tag="PK710_07815"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HOT09660.1"
                     /translation="MPHPPLYLSPPAVLLPATRLTNEQVIARVRENYRGNPIKWPLLE
                     AAMRRVLERCGTSIRYFEEDHEARVGDYAARAANLCLQTHRVASSDISLLIYGGVARE
                     YFEPATAMEVAAKCGLRHVHAFDVTSACAGQLEGIHIAASYLSMHDEYRMALIGSAEL
                     TRNFLCFDIQTVKDLLTKAAGLTIGNAASAWLLGRQPFAGGSLRLLGFRNHSMPTHWH
                     LCSAPIDGTFTSLSTDLFKLNEHVAPEVERLVAQVGWKVDDVDHFVFHQPSESILLGV
                     LDDLGVDRSKGLLTHHLYANTVSTTVSLALHELLKQKRVRPGDKLVLGTAAAGFSMVS
                     LAGVWEA"
     gene            complement(5449..6816)
                     /locus_tag="PK710_07820"
     CDS             complement(5449..6816)
                     /locus_tag="PK710_07820"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HOT09661.1"
                     /translation="MHLRLLALFHGAALVAACFPLGCSGDDPTVEGVSSLKGIEITAV
                     SPRNVRLRWQPVEDGQVIIHRISSGNKVQEVGRKPASHGRFLDLALEPESSYSYRLTL
                     CHQDSCEPPFQTGSVSTPESGFPSIETTVPAVNSSDDVAIFGVYRIAAELFREGRMAA
                     VDRTGSIVWEYVTYEWGPITEVQPLADGTIATGQNQYLVHIDLDGSELFRWTQTTARH
                     DIDRLSDGRWAFLFFDPFEVDGKTRLGDGIVILNQQGTAVEWKWAGRDHIPWTDVNEQ
                     DLASNELGLGQDWTHSNAITFDETGSKVLLNVRNLNRIYKIDVATQKVDWIMGDGGDF
                     GAGIWDHCHDPQFLSDHRVLIFDNGYRRPKPQYSRVIEVEFDPEQKQAQIVWEYRETP
                     DFYSFALGSAQALDNGNVLVTDGINGRVVEVTRDKHKAWEFLVQKYYWIYKAVNVPRS
                     FFTDW"
     gene            complement(6908..7582)
                     /locus_tag="PK710_07825"
     CDS             complement(6908..7582)
                     /locus_tag="PK710_07825"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOT09662.1"
                     /translation="MIGIVAALVLSLVGVTVAFYPGLSGSPWVWVSLGGSQLPIAAYS
                     LKNIIKNNELQDRLGPRWGDISLGMGSALLLFAASWMGRTVMAPNGSARTQWLARAYA
                     QLGASDALEKHWMLVLVGVCIIAIIEELGWRGWVLPMLEQRHGTRLAWPLTGLLYAVA
                     FLPTVWWLRSDSGPNPFVVGAALLCGCTWSYLAAKTQRLLPSILSHAVFVWFIVLQFR
                     MLNLDK"
     gene            complement(7584..8816)
                     /gene="coaBC"
                     /locus_tag="PK710_07830"
     CDS             complement(7584..8816)
                     /gene="coaBC"
                     /locus_tag="PK710_07830"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOT09663.1"
                     /translation="MNQPVTTPHSPTVVLGVTGSIAAFKSIEIARLLIQHHIRVLPVL
                     TQSGARFVGPVTFTGICGEASRMDMWDPSFSGELHVALASQADVVLVAPATADIIARF
                     AQGRADDLLTALTLCARGHVIVAPAMHPRMWAHPATQRNVQTLQRDARIEWVGPVVGP
                     VANGEIGLGRMAEPQNIVSAVLARLRPPDLNDWHIVVTAGPTVEDIDPVRFLGNRSTG
                     RMGFSVARRAAARGARVTLIAGPTELTTPAGVQRRDVRSASDMRSTLHDVLGPSLGGA
                     DALVMAAAVGDYRPAHIHNEKMKRGAQPIQLELVANPDILAELGSMRTGSSPLLVGFA
                     VETADEPTLIEHARGKLSKKRVDFIVANSASIGFGGESNEAILVGSDDIERLPRMTKD
                     ELADRILDRVIGLRSGRG"
     gene            complement(8889..11486)
                     /locus_tag="PK710_07835"
     CDS             complement(8889..11486)
                     /locus_tag="PK710_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09664.1"
                     /translation="MIRRNAILIGLFLVSAASLLFEILLTKFFGSKLEHHYTFAIIST
                     AMLGFGSAGVFVHLRPGSFPTDDGPSPKVLGRYAYWFALSCLIVIPVFCFLPVDPLFA
                     DWRGVIALPLFFLMFALPFFFVGVCVSYTLIAAKQGPGIIYFWDLLGAGLGAAVAASA
                     ISSLTGYGATVMVALLGVAGALFYWHATGSLPWKYSLPWRIPLAIALCVGVLAYPGLA
                     RGIYGHDIFSMKYGLLRKALLVDFQGIEMSFWNAIARIDVSKTGDSRERSYRGGIAHR
                     FDTVDLPGRFILVDGGASTRQFALHAPAPEQEFLKNSLWAAPYVPRSADSPPEKALVI
                     GAGGGVDVLVAKAFGTKHVDAIELNPDTYRVLLGLPQDPMRDRYSPWILSDERTSVNL
                     LNTEARHWCHTLGGEETYDVIEASGVDTLTAIRSAANALSENFLYTEDAVADYYRLLK
                     PGGILSLSHGYSSPASLTLRKFVTYLTFLEKHGVQRPGDSLVFIFDSYWENGLLKKGA
                     FTKDEIARLEAYAAANHYRFIYHPLQPDDTPPVRPLGDAFSKLDDATYDELAKYYAPF
                     IDPLRETVTQKDLPFIPLARAANAEEREKILSQMRWDVRPSTDERPYFYFVGTNQHTW
                     LSAIDGAFVYPQPAVRWMFLCALLASAALVAAPILVRRKAKLEGGNATARKQVVAALP
                     FFALSGFAFMLVENVAFLNLTLFVGGPLYSLSIVLPSVLIGYAFGSLATEKYLVRSSR
                     SFGIMVGLFVLGAVLYASAIRFGLPHLIGTSWGLRVFASIVLTLPLGALLGVPVPWYM
                     SSLKTSDARSLAWMWAVSSAFNVLGSMLFVPICFAMGRTPITLFAAAIYLAAILWAGW
                     TRQRYPAEA"
     gene            complement(11738..>12173)
                     /locus_tag="PK710_07840"
     CDS             complement(11738..>12173)
                     /locus_tag="PK710_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09665.1"
                     /translation="DRRFADDETFGRRKAEPIWKQVVLSRGWDENTVLARGQRVSFEN
                     PVLNLNRWATAEVLDGVPDRWLRFCLSVAEVTGLRHFGLDLRVPAGEDEDWREVSPDK
                     TVIIEVNASPALVQLHDLGCQDKAIAGQARVLRAAFSSPSVC"
BASE COUNT         2862 a         3307 c         3581 g         2423 t
ORIGIN      
        1 cgacgagcac cagcgaactt acgcccgcca cggtgattca cgaacccgag gcaccccaac
       61 aggcaccacc ggtcgaaacg ggtgaggcag ccatggggga gccagaagac gcggagacag
      121 gggagaaccc cgatggttcg caggaagaag gtcctgccgc ggcggcctct tccgcatcaa
      181 ccgcggttcc gacgcctacc accgtgccaa cgcgcaagcc accgattgag gttcgggttc
      241 atcctcccaa accggttcca ccaaggcgtg acgggggtta ttgattcgac ggactggagc
      301 aggttttcaa caaggtgccc gttcgatcat gagcggcgaa aaaacgtatg gacgagttcg
      361 tcgccacgca catcgctata ggttgcgtac ccctttttct ccgccatatc cacgagcgga
      421 gcgggaacga agggagtgat gagaaggtat acctgtcccg gagcgagttg attcagcgcc
      481 gaaaggactg gttgcatggg atggccgccc gcatcgagca gaggacgagc atcgaatgat
      541 gcggtaactt gttctggatt ggcccagggg ggagcgggca gaggttcttg ttgaggttgc
      601 gcatcggtgg gttgttgttc ttggccgacg gcttctcgca ggcgattgat caagatgcca
      661 aggccaatgc ccccgacgga agcaacttgt ctcaaggtgg cgaccttggc cacggtacga
      721 cgaagtactg gatttttcag ggcttgaaag gtgggagaca agctcacgag gacggcttct
      781 agttcggggt atacctcgag cagttgagcc acacgaacat cgggagtgat gagcaaccgt
      841 tcattcgcca tgctcgacct cacgttccca agtgaggagg cgctgttcac cttgcagtgc
      901 ccgtttgtcc gtgaggtctt ggctgacttc gatacatccg aggtattgac catcggcatc
      961 gcgcatggcg aagtattcga tggagatgaa gcgtcctttc atttcgatcc agaaggccgc
     1021 ccgatctttg ttaccgttgc ggaattgttc gagaatttgt tcgaccgtat gaacgctcga
     1081 gggcggatgg caatactgga ctttgcgtcc gaggatggcg cgcgtgcgag cgaagatacg
     1141 ttctgcgcct tgggtgaagt agcgaacggt atccttggca tccacgaagg tgatgtcgaa
     1201 aggaatggta ttgagcatgg cttctagttc agccaggtcg aagcttcccg atttgagttg
     1261 aatacgaccg ctttgcgatt cttgcgcgag agattcgggc agatcgtcag ggcgccattc
     1321 actcggagga tcgtaaagac aaaatccgat ctcggaactc tggcgtgcga tttcaaacca
     1381 ttcctgggac gacaacgtat cgaggcacat gggaaaaaga atttgttctt ctttatccgt
     1441 catgtcggtg atggctttgc atgcgggagc aagaacgagg tcgatcactc ctttggcttc
     1501 gcccgcggtg acggtgcgag tggaggccag ggcttcggcg gcggacttga gcaacgcgcg
     1561 ggtttcgtcg tgcttgcccc acatgatttt gggtggaccg gtgatgccgt gtttttcgag
     1621 gaaggggaag agcagatgtt ctttgcgaag gtagtgtttc tcgacatcca tgagggcgtt
     1681 gaaacggagt cgtaaggcac tccacacgga ggagacgtct tgttgagggt cggcgacggt
     1741 gagatcggca ctgagttttg cgacttgttt gacttcgagg cgcaaggctt ggttttcggc
     1801 ggcaaaggtg tggacggggt gtccggtggg cagtggtttt tttggttttc ggatttgcac
     1861 gagaccggag agggcttcgg catggacatc gcatagttcg agcacttctt tttcgggaag
     1921 tccctcggcc atcaactctt gttcgacctc gacgacatcc ccataaggaa cttcgcccaa
     1981 gaggcgagca agttgcgccc gcaccgcttg tggcgccgta ccgtcgtgaa gctggcgaat
     2041 catgtgcttg agcagttctt tgcgacgcgt ggcgttgtcg atcacctcac tcatcgtgct
     2101 gatctcctgg ggttgtgggt gggagcagaa agaccaatcc gggcgttact atgcgtcgat
     2161 ctcgtgcaag cgcaaccgtc gcgcaaagat catccataag ggcaacggca aggcgcatgg
     2221 ttcgagcaag ccatcgtatc ggatcagcta tcgggaatcg tagatattca tggccggaag
     2281 ctcgagagcg gaaaggtacc ccccgtgtcc acatcccgta tcgatcccga tggcgtcgcc
     2341 gcaacgccag atgccaatct gatcgttcat gacacctttg gggatttctt gggcgaggta
     2401 ttcagtcccc gtgtggccaa aaacaaccag ctttcccgta tagttagaaa agaattcctc
     2461 atctcgaccc cacagcaggc tcatggagtc ggtcacttgt cgagggtgat aaaaccctcc
     2521 atccggatgc tgtggcagcc ccgcatgaac atagatggcg tgctcatctt cgtaccacag
     2581 gggcaattgc cgaaaccatt gcaccacatc gtcgggaaaa aaagcccctg tctgaatcat
     2641 catccgttca tcggcccggg gtagttcgtt ttcggagggc accggtccgt cgacaaaaga
     2701 tcggaacgcg gcgaggcatc cgttgagtgg cggcatgacg aattcgtccc atccgtggtc
     2761 cacgacccga agccaagcgt cttcgtgatt tccgcgcaag gccaccacgt tcgcgcgggt
     2821 catcgacgga agatttctaa tataatcaac aacttgcgca gacttgggac cacgatccac
     2881 atagtcaccg atgaacacga gggtgtcttc cgcatcgagt tcgggaaagc atgaaaggag
     2941 acgaaacaac tggtcgaggc caccgtgtaa gtctccgaca gcgaacgttc ggccggccat
     3001 tggagcgcat cttgacaggt ggggcggtac aagggaagcg caagaggcga tgggtcgata
     3061 gcatccatcg gatagacaaa aaagctgtcg cgcggagagc ctcggggggg gcagtcatcc
     3121 gcgcgacaag caaaagcgcg aaaggacaga gggggggagg gaggggtatg tctctctcgc
     3181 gccgatgttg ggatagcata tcgtgggggg aaaaaccaag atttggttgc gtgggcgcgt
     3241 tggcagcagc gaggcggcaa gggactagct tgccgtcttg gaattttcgc gacttacgac
     3301 ggttcagggg caagccaggt cgaagtcatc gatgacgaag gcgaggtcgg aacgaatttt
     3361 ttctgcggtc aacccgaatt tttcgagact gtacgtatga gggctttttc gttgtcgttg
     3421 cacgatcgct tgctgttgca cccgagtccg gaactgggtg gggggttcga tttcgagaaa
     3481 gtcgacaatg tcgcgcatgg tggattcgag gtcggccatg agttggggat agcgcacgat
     3541 gagtaggttt ttttccggga tggcgccttg tttccacacc tcgtggaagt accggaacaa
     3601 gtaacaggaa gcctgataaa gattatcgag atatcgtttc cggtcttgct cccgggtgct
     3661 gtgaaatacg tcgtaggatt gctcgagcac gccggtaatc atggacatgc cgctagggat
     3721 cgtttcgacg ggatcgcgga cgacatacag cagtttgcag tccggatagc gatgcaacag
     3781 cgttttgatg cgcaaggtgt agctgcttgc tttcaccgcg atgcgcgatt tgtttttgac
     3841 gagcaggttt cttcgccaac aaccttccat atagtggaag tgtcgttcga tgttttcggc
     3901 tgaatcttgg tcgagttcga acaaggcccg ggcagattcg cttccccaat ggtcttgcca
     3961 agcgaggaag taggtccaca aaaacgcgcc atcgacgaag cgaaacaggg cgagggcgtc
     4021 gtccgtttcc acgtcccgca agctggtttc atgagcagcg gcgctgtgat aacgggctgg
     4081 agaaagaggc gcaagtcgat cgacgaacgg tccgatggtg cgacgagcgg tgatggcggg
     4141 aaagagcatt tcccacagtt cgaaagcagc caaggagtct gtgtggagca agaatcggtg
     4201 caggaacgtg gtgccgctgc gtgggttacc gaggatgaac acggggcgat cgatcggttg
     4261 tcgagtgaag gacggcgacc acagatggtc ggcggcgagc gttgcggcgt tgacaacgga
     4321 atgggcttgt ttgagagcga aggtaccgat ggggcgaagt cggaagccga aggttttcag
     4381 gatgatccgg tagatttgga ggtaacgatt catcttacgc ttcccagacg ccggccaggc
     4441 taaccatgct gaagcccgct gctgcagtgc caagcacgag tttatctcca ggacgtacgc
     4501 gcttttgttt cagcaactca tgcaaggcca aactcacggt cgtgctcacg gtattggcgt
     4561 aaagatggtg cgtcagaagc cccttgcttc gatccacacc gaggtcatcg agcactccaa
     4621 gcaaaatgct ctccgacggt tgatggaaaa cgaaatggtc cacgtcgtcg actttccatc
     4681 ccacttgcgc caccagtctt tcgacttccg gggccacgtg ttcattgagt ttgaatagat
     4741 ccgtggacag ggacgtgaag gttccatcga ttggggcgga acacagatgc caatgggtgg
     4801 gcatggagtg attgcggaat ccgaggagtc tcagacttcc tcccgcaaaa ggctgacggc
     4861 cgagcaacca tgcgcttgcc gcatttccga tggtcagacc cgccgccttc gtgagaaggt
     4921 ctttcaccgt ctgaatatcg aaacaaagaa agtttcgcgt gagttcggcg gaaccaatca
     4981 aagccattcg atactcatca tgcatggaaa gataactggc ggcgatgtga atgccttcga
     5041 gctgtcccgc gcatgcgctg gtgacgtcga aagcgtggac atgccgtagc ccgcacttgg
     5101 cggcgacttc catggccgta gctggctcga agtattcgcg cgccacacca ccgtaaatga
     5161 gcaaactgat gtccgaggat gcaacgcggt gggtttgcag gcataggttt gccgcgcgag
     5221 cggcgtagtc tccgacccga gcctcatgat cttcctcgaa atagcgaatg gaggtgccac
     5281 aacgttcgag cactctacgc atggccgctt cgagcaaagg ccacttgata gggtttccac
     5341 gatagttttc acgaacgcga gcgatcacct gctcgttggt aagccgcgtg gcagggagaa
     5401 gcaccgcggg aggtgacaag tacaaaggcg gatgcggcat gagctaggtc accagtccgt
     5461 gaaaaaagat cgcggcacgt tcacggcctt gtagatccag taatacttct gtaccaaaaa
     5521 ttcccacgcc ttgtgcttgt cccgcgtaac ttccaccacc cttccgttga tgccgtcggt
     5581 cacaaggacg ttgccattgt ccaaggcttg tgccgaaccg agagcaaacg aataaaaatc
     5641 cggtgtttca cggtactccc agacgatttg tgcctgtttc tgttcgggat cgaattccac
     5701 ctcgatcaca cgtgaatact ggggttttgg gcgacgatat ccattgtcga agatcaacac
     5761 gcggtggtca gaaagaaatt gtggatcatg gcagtggtcc cagatcccag cgccgaaatc
     5821 tcccccatcg cccatgatcc aatccacctt ctgggttgcc acatcgattt tgtaaattcg
     5881 attgagattc cgaacgttga gcaacacctt gctgccggtc tcatcgaagg taatcgcgtt
     5941 ggagtgtgtc caatcctgtc cgagtccaag ctcgttggag gccaagtcct gttcgttgac
     6001 gtcagtccac ggaatatgat ctcgtcccgc ccacttccac tccactgccg tgccttgctg
     6061 attgagtatc acaatcccat cgcccaaccg cgtcttgcca tccacctcga acggatcgaa
     6121 aaataaaaat gcccagcgac cgtcggataa ccgatcgatg tcatgccgcg ccgtggtctg
     6181 cgtccaacga aacaactccg agccatccag atcgatgtga accagatact gattctgacc
     6241 tgtcgctatc gttccatccg caagaggctg cacctccgta atcggtcccc attcgtacgt
     6301 gacatattcc cagacgatcg atcccgttcg atccacggcg gccattcgac cttcccgaaa
     6361 tagctccgcg gcaattcgat acacgccaaa gatggcaacg tcatcggatg aattcaccgc
     6421 cggcaccgtg gtttcgatgg agggaaagcc gctttcggga gtcgagacag acccggtttg
     6481 aaacggtggt tcacaagagt cctggtgaca caaggtcaac cgatacgaat acgaggactc
     6541 cggctcaagg gcgaggtcga gaaaccgacc gtggctcgct ggcttgcgcc ccacttcctg
     6601 aaccttgttc ccggaagaaa tccgatgaat gatgacctgt ccgtcttcga ccggctgcca
     6661 acgaaggcgc acattgcggg gactgacagc agtgatttcg atacctttga gcgacgatac
     6721 gccttcgacc gtagggtcat cgccactgca gcccaacggg aagcaagccg ccacgagcgc
     6781 ggcaccatga aatagagcaa gaagacgaag atgcacgggt ggcaggatag cgcccgaatc
     6841 ccgtggaaca aggcagttcg tgcaacaagg aggcatggga agaaaagggg cttgtgacgg
     6901 aagataccta tttgtccagg tttagcattc gaaattgcag gacgatgaac caaacgaaaa
     6961 cggcatggga caggatggag ggaaggagac gctgggtttt ggccgcgaga tagctccacg
     7021 tgcaaccgca taacaatgcc gccccaacca cgaatggatt cggtccggag tccgagcgta
     7081 gccaccaaac ggtaggaaga aacgcaacgg catacaacag gcccgtcaaa ggccaagcta
     7141 gacgagtccc atggcgctgc tccaacatgg gcaacaccca gccgcgccac cccaattcct
     7201 cgatgatggc gataatgcaa acacctacca acacgagcat ccaatgtttt tccaaggcgt
     7261 ccgatgcccc gagttgcgca taggctcgcg caagccattg cgttctcgcc gaaccattcg
     7321 gagccatcac ggttcggccc atccacgacg ctgcaaaaag caacagggcc gagcccattc
     7381 ccaaggaaat atctccccag cgagggccca gccgatcttg taactcattg ttttttatta
     7441 tattttttag cgaataggca gcgatcggta gctgggaacc tcccagcgat acccataccc
     7501 acggcgaccc ggacaggccc ggataaaaag ccacggtcac accgaccaac gacagaacca
     7561 gggccgcgac gatcccaatc acgtcaacct cgcccggatc gaaggccaat cacacggtca
     7621 agaatccgat cggcaagctc atctttcgtc attcgaggta agcgttcgat atcatcgcta
     7681 cccacgagga tggcctcgtt ggattcgcca ccaaagccga tcgaagcgct attggccacg
     7741 atgaagtcca cacgcttttt ggaaagcttg ccgcgggcat gctcgatcaa ggtaggttca
     7801 tcggcagttt cgaccgcgaa tccgacgagc agcggtgacg aacccgtacg catggaaccg
     7861 agttctgcga ggatgtccgg gttggcgaca agctccaact gaatcggttg ggcacctcgt
     7921 ttcatttttt cgttgtggat atgcgcggga cgataatccc ccacggccgc ggccatcacc
     7981 agtgcgtccg cgccgccgag cgaaggaccc agcacatcgt gtagcgtgct tcgcatgtcg
     8041 cttgcggacc gcacatcgcg acgttgcacc cctgcgggtg tggtcaattc cgtgggaccg
     8101 gcgatcaagg taacgcgagc gccccgggcg gcggctcttc gcgccacgga aaagcccatg
     8161 cgtcccgtgg atcgattgcc gagaaaacgt acgggatcga tgtcttccac ggtaggtcca
     8221 gccgttacca cgatatgcca atcgttcaag tcaggtggtc tgagacgcgc cagcacggcg
     8281 cttacgatat tttgtggttc tgccatgcgc cccaagccga tttcgccatt ggctaccggg
     8341 ccaacaacag gtccaaccca ctcgattcgc gcatctcgtt ggagcgtttg gacgttgcgt
     8401 tgggtggcgg gatgggccca catccgtgga tgcatggcag gggcgacgat gacatgccca
     8461 cgggcgcaaa gggtcagagc ggtcagcaag tcatcggcac gtccttgcgc gaagcgagcg
     8521 atgatatccg cggttgcggg agcgacgagc accacatcgg cctgagacgc cagcgctacg
     8581 tgcagttctc cggagaagga aggatcccac atatccatgc gtgaagcttc gccgcaaatg
     8641 ccggtgaaag tgacaggacc cacgaagcgt gcgccggatt gggtcaacac gggcaagacg
     8701 cggatatggt gttgaatcag cagacgggcg atttcgatgg acttgaaggc ggcgatgctt
     8761 cctgtgacgc caagcaccac ggttgggcta tggggggttg tgacgggctg gttcatggcg
     8821 gcgggcagcc tagcgtgaat cccatggcag cggccaggtt tggtgaacgg ttgtctcgtg
     8881 aatggacctt aggcttccgc cggatatcgc tgccgcgtcc atcctgccca taagatagcg
     8941 gcaaggtaga tcgcagcggc aaatagcgtg atgggcgtgc ggcccatggc aaagcaaata
     9001 ggaacgaaaa gcatcgaacc aagcacgttg aaagcggaag ataccgccca catccacgcc
     9061 aagcttcgcg catcactcgt tttgagggaa ctcatgtacc aagggacagg aacgccaagc
     9121 aatgcaccaa gcggcaaggt cagaacgatg gacgcgaaca cacgcaatcc ccaactcgtt
     9181 ccgatgaggt gaggcaaacc gaatcgaatg gccgatgcgt acaaaacagc acccagaacg
     9241 aataacccca ccatgattcc gaaactacgc gaagaacgaa ccaggtactt ttccgtggcc
     9301 aggcttccga aggcgtagcc gatgagcacg ctcggcaaaa cgatggatag cgaatacagc
     9361 ggtccgccca caaacaaggt caagttcaaa aacgcaacgt tttctaccaa catgaaggcg
     9421 aaaccggaga gggcgaaaaa gggcaaagcg gcgacgacct gctttcgcgc cgtggcgttt
     9481 ccaccttcga gcttggcttt gcgccgcacc aggatcggcg cggccaccaa tgccgcggaa
     9541 gcaagcaacg cgcagaggaa catccatcgc acggcgggct ggggatagac gaacgcgcca
     9601 tcgatggcgc tcaaccacgt gtgttggttg gtaccgacga agtagaaata aggacgttcg
     9661 tcggtggaag gtcgtacatc ccagcgcatc tggcttagga ttttttctcg ctcttcggcg
     9721 ttggcagccc gtgccaaagg aatgaaagga aggtcttttt gcgtcaccgt ctcgcgcaaa
     9781 ggatcgatga acggtgcgta atacttggca agctcatcgt aggtggcgtc gtcgagcttg
     9841 gaaaaagcat cgcccagagg acgaacggga ggcgtatcgt cgggttgtaa ggggtgatag
     9901 atgaagcgat agtggttggc ggcggcgtac gcttcgagtc gagcgatctc gtctttggtg
     9961 aatgcgcctt ttttgagaag cccgttttcc caatagctat cgaaaatgaa aacgaggcta
    10021 tcaccggggc gttgcactcc gtgtttttcc aaaaaagtca aataagtcac gaacttacgt
    10081 agtgtcagcg aagcggggga cgagtagcca tgagacagcg acaggatgcc tcccggcttg
    10141 agcaaacggt agtaatcggc caccgcatcc tcggtataca agaagttttc cgatagcgcg
    10201 ttggcagcgg atcgaatcgc ggtaagagtg tcgacaccac tggcttcgat cacgtcatag
    10261 gtttcttctc ctccgagggt gtgacaccaa tgccgcgctt cggtattcag caagttcacc
    10321 gaggtgcgtt catcggataa aatccaagga ctatagcgat ctcgcatcgg gtcttgcggc
    10381 agaccgagca acacacggta ggtgtcggga ttcaactcga tggcatcgac atgcttggtt
    10441 ccgaaggctt tggcgacgag cacatcgacg ccaccaccgg caccgatgac gagtgccttt
    10501 tcgggtgggg agtcggcaga gcgcgggaca taaggtgccg cccacaacga gttttttaga
    10561 aattcctgct cgggagccgg ggcgtggaga gcgaattgac gcgtgctggc acctccgtcc
    10621 accaaaatga agcgaccagg aagatccacc gtatcgaagc gatgggcaat gcctccccgg
    10681 tacgatcgct cccgagagtc tcccgtcttg ctcacgtcga tgcgcgcgat ggcgttccag
    10741 aaggacattt cgataccttg gaaatcgacc aacagagcct tacgcagcag cccatacttc
    10801 atggaaaaaa tgtcatgccc gtagattccg cgcgccaagc ccggataggc gagcacgccg
    10861 acacaaagcg cgatcgccag gggaatgcgc catggcaacg aatacttcca gggcagggag
    10921 ccggtggcat gccaataaaa caaggcaccc gcgacaccga gcagggccac catcacggtg
    10981 gcgccataac ccgtcagaga actgatggcc gatgcggcga cggcggcacc aagccccgca
    11041 ccgagcaagt cccaaaagta gatgatgccg ggcccttgtt tggcagcgat caacgtgtag
    11101 ctcacgcaaa cgcccacaaa gaaaaagggc agtgcaaaca tcagaaaaaa cagcggaaga
    11161 gcgatcacac ctcgccaatc cgcaaacaac ggatccaccg gcagaaaaca aaacaccgga
    11221 atgacaatca aacaggataa cgcaaaccaa taggcgtatc tgcccaacac cttcggggaa
    11281 gggccgtcgt cggtgggaaa agagccgggc cgaaggtgaa cgaacacacc cgcactgcca
    11341 aaacccagca ttgccgtgct gatgatggca aacgtgtagt ggtgctcgag tttcgagccg
    11401 aagaacttcg tgagaagaat ctcgaacagc aacgaagccg cgctgacgag aaacagtcct
    11461 atcaggatcg cgttgcgacg aatcacgtcg cgaagctagt tccgagcggg cggactgtat
    11521 agcgggtttc ccatttccga ccgataacac tcatattttt ggggaatcgc gaaaggggaa
    11581 cccgtgaggc gacagtccat attggcgatg acgttgcggg aagggcaatc atcgactcgt
    11641 cgaatcagtt tggtgatctc aaatttcgga ctccgccgtc gagcccagca gccattcgat
    11701 gtcaccgctt gtgggaggaa ttcggctgct cggtatttca acaaacggat ggggaagaaa
    11761 atgccgcgcg aagcactctt gcctgacccg caatcgcctt gtcttggcat cccaagtcat
    11821 gcagttgcac aagcgctggc gaggcattga cctcgataat gactgtttta tccggggaaa
    11881 cctcccgcca gtcctcgtcc tcccccgcgg gaacgcgaag atccaatccg aagtgccgca
    11941 atcccgtcac ctcagccacg gacagacaaa atcgaagcca ccgatccggc acaccgtcca
    12001 acacctccgc ggtagcccag cggttcaagt tcaacaccgg gttttcgaaa gacactcgtt
    12061 gtccacgagc cagcacggtg ttctcatccc atcctcgcga tagcaccact tgcttccaaa
    12121 tgggttcggc ctttcgacga ccaaacgtct cgtcgtcggc aaatcgccga tcc
//
LOCUS       DAOSOR010000105        12108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5233_length_12108_cov_4.372272, whole genome shotgun sequence.
ACCESSION   DAOSOR010000105 DAOSOR010000000
VERSION     DAOSOR010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 12108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12108
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5233_length_12108_cov_4.372272"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1695
                     /locus_tag="PK710_07845"
     CDS             <1..1695
                     /locus_tag="PK710_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09666.1"
                     /translation="KVIAGTIPNGKVFVLEGQGAAKELVSLPDTEHVWALALDPKSKA
                     VFAATGPEGKLFRLGADGSAQVHFDSDEAHLISLVAAPDGTLYAGSSGNALLYKITGP
                     GRATVMYDFPGDDVKALALAKDGRLFAIANEHQTPPIIPRTNQSNADKSVGASKSDRV
                     VPKPGKGRLVRFDTHGRAEELLFNADSHFQALALGDDGIPYVGTAKDGEVYAVDDAHT
                     SMLVADTPERQVGAMVLAGNTRFVATTDPAVFHPIRAVGGTESVWNSAVLDAGLRATF
                     GQLTWRATGPVELSTRTGNTEKPDASWSPWSPAMAAPSKMASPPARYLQIRARFSRDP
                     SAIVREITAYFVTDNARAVVTSVTVGEPGSSDTKKSDSVPESGGDLAEAKSKLKLSWK
                     VNNPDNDSLRFRLFYRFEDATVWRPILPAHEILTKTSYEWDTAGLPEGTYRVKVEASD
                     ELANPPLQTLRHSLESGTVLIDNTPPLIQQLTVAGKIIRGVAVDGVGPIARIDVAVDP
                     NLSLWTPFFPADGIFDDRSERFELDVSSLLASGSGLVAVRVSDAAGNRVVRTVDIR"
     gene            1850..2866
                     /locus_tag="PK710_07850"
     CDS             1850..2866
                     /locus_tag="PK710_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09667.1"
                     /translation="MRPHTSILFLLSMTFLVACGELSSLEAAETNATCPDGCFNADAG
                     TDAPVSDVTADIGHDGVGETATLSNPLCGTLVCDPDDPGALDCYGSGPGIDAGSGIEL
                     DAAYEAGEKDDDHGGGKMGGAPPEKPHPPPDNPQDKPQRMSCQVAADGFGQRETTCVA
                     AGTGIDNEPCVSSADCAAGHTCVGDINVGVCRAYCCLNAESCVEGTYCGIMRSRDALT
                     ADPESELLLPVCIPASDCELLPGAEGNNRCAEGLMCSVVRSNGTTACVLPGTAVEGEP
                     CGPSDSQKSPCAEGFVCSKTTNTCLALCRVGDPTACGNGVCQSGTGGLPEPYGICVGT
                     RSAP"
     gene            2863..3480
                     /locus_tag="PK710_07855"
     CDS             2863..3480
                     /locus_tag="PK710_07855"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOT09668.1"
                     /translation="MKRVSTLLFDVMGTLVFDPFHDHVPAFFSLTFEQLLEQKHPTAW
                     KEFERGSIDEPTFFPKFFRDGRSFDTNAFKAMMKQYYAWIDGMEALVSELFDTGVPMH
                     ALSNYPPWYSMIEQRTGLSRFVAWSFVSCHTGLRKPDPKAYLHAAQELGQPMDSLLFI
                     DDRPINCDAARSVGMQAIDFVDARQLRTEFFERGLLADRQVRRRP"
     gene            complement(3464..3859)
                     /locus_tag="PK710_07860"
     CDS             complement(3464..3859)
                     /locus_tag="PK710_07860"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOT09669.1"
                     /translation="MEMHITFAGGKRVNAEWLGRTIATDQAREGGGDGSAPEPYALFL
                     ASIGTCAGIYVLEFCRARNLPTEGISLVQRLGFNPVTHKLENVRIEIRLPPDFPEKYK
                     KPVQRAADLCAVKRTILEPPNFEVVTVDA"
     gene            complement(4300..6879)
                     /locus_tag="PK710_07865"
     CDS             complement(4300..6879)
                     /locus_tag="PK710_07865"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOT09670.1"
                     /translation="MSERNIPPILRRLGQLQADHPWRFLAIAFLSLLPALYGIFGAGG
                     LGFKSSFDELLPDNKDSVIEARRVGERLAGASTLTVVAETRAHPNPEALKRFVDALVP
                     KLEALGPDKVGAVDFGVKKTQEFFDHNKVLYAQLEDIQKAHDELISRYDYEVAKARGD
                     FIDDDDVPTPLTADSIEEKIRGKSSGSATDAVTGRYRDGYYMNPEGNFIAVLIRTSVS
                     GKEAKAEFRSEVENVVASVNPSRFDPSMEVNYTGNFITSAEEYDAIIGDLMQVGGWGL
                     AGVLGAVLLFFMRIRTVAAMGMTVMIALLWTFGLTRLTIGHLNSSTGFLVSIIAGNGI
                     NYSIMFMARYLEARRDEAADPKNAILVAHRDSWIPTLAGSATAMLAYGSLAITDFRGF
                     KHFGVISGYGMILCWFATYLFLPAILAVSERLVPSFRKTEKPTVMRGYYGVLFAKLAR
                     FAPRPIAIVGAIMGAVAVVAAALYLIEDPMEYDMANVRNERKDRTSAGLLSVRVDKVV
                     GRMGQDGMAIMTDRLDQVPMLEVELNRRWKEAPEDLKPFEKVVSIFSLIPKDQPEKIK
                     LVSEMRQRLQRARDRGFINDADWERLQPHIPAEDLQPITVESLPEQVARPFTERDGTR
                     GRIVFIVPKEGFSVWDAHYLMRWADSYRSTPLPTGEVIKGSGSAVIFADMIKTVGEDA
                     PKAITVSAAGSILVILLAFRFRRQAWGVFLPWLGGVAGLLAFLYFANIKLNFLSFVTI
                     PITIGIGAEYAHNLMQRYRIEGPERVYRVVVETGGAVILCALTTTLGYLALTLSINRG
                     IVSFGLAAAVGEVICTLAAVLVLPAFLFWFADRQKKSHKPKEIAPSVFSFAAREQPCP
                     SDK"
     gene            7220..9118
                     /locus_tag="PK710_07870"
     CDS             7220..9118
                     /locus_tag="PK710_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09671.1"
                     /translation="MAKSRRRSIYPILLIILANGLVFSGSMVPLAYWGFEDGPPVDVA
                     AAGFAGWLIMALSVLGLAIMILGGVLLRAVPRIPAFLLGLFPAFVAGVGVLGAQIGLG
                     NVFEAVGVAGADRIQVAALGVFEALNASVIGAYVAAALMMASALTISLRHWPSRGSLS
                     SGGKVGLVGGAFLLIAMIIGSLFWTPLGGLGVLPWITAFAGLVAIAVAAHAIRNDPTD
                     AANVQAAGELWVTLLLSMGAIVWVVTARHTSALMEGSMTLSASSPHLDPSKVIESWTA
                     AGPALYASALYVVPLLFAAVMSMLSRESLGSWGLRKAASSLLFVPLFFAVPAVLQHLQ
                     TGSAAKRLADVLSCAPAWLPRVQQTLPITKNAMESSCPEAVLEIGPSEIRLGDKNLGA
                     LSKLDSSDGCAAVSSQLVEFQGFFAVSSTLSYRRAACLIDALSQSPSFRGRSDMYRQR
                     NPESSAGRAGGVVFWLLQSEPRGEASARPPFDQLGTRLWQLTTFGPSAEGWDPRLVHL
                     HVMENGWTLKRDPASTPMRFEGSAVDGLSKLQSVLGLNMAAQVMVLSAEGAVSMGQLL
                     HYASAFRDPQLMMPAVEPSDNALPDDKDAGVGDGDSPEAIAPEAAATPAASASSAGSA
                     SSAVGPDR"
     gene            9174..9530
                     /locus_tag="PK710_07875"
     CDS             9174..9530
                     /locus_tag="PK710_07875"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOT09672.1"
                     /translation="MKTLIILNDPPYGTERTYNGLRLAANMLKMDEQEPEMVVFLMGD
                     AVSSAKKGQKTPNGYYNLERMIELVLKRGAEVWLCGTCMDARGIGEDEVVQGARRSTL
                     DALTQATLEAQKVLVF"
     gene            9530..10663
                     /locus_tag="PK710_07880"
     CDS             9530..10663
                     /locus_tag="PK710_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOT09673.1"
                     /translation="MASIYLDHNATTPLHPDVVEAMLPYLTTHFGNPSSGHEVGLRAR
                     AAVEAAREQVASLLGCSSEEVVFTSGGTESNNLALRGLTATHAGAMHVLTSAVEHPAI
                     EMPCRQLERQNVRVTRVAVDSFGRVDPGAVVQALERDTVLVTVMLANNETGTLMGVRE
                     IAHEARARGVIVHTDAAQAVGKVPTRVADLGVDMLSIAGHKLYAPKGVGALYLRKGIK
                     ISPLLFGAGHERGMRPGTENVASIVGLGAACALAERDGARQADRQRGLRDKLWELLKE
                     KIPGLSLNGHPSERLPNTLNVSFPGVKGSEVLARAQDVAASTGSACHAGSETPSTILK
                     AMGISDAVALGAVRLSLGRETSEDDIVRAAASLVHAYDAAAKA"
     gene            complement(10706..11650)
                     /locus_tag="PK710_07885"
     CDS             complement(10706..11650)
                     /locus_tag="PK710_07885"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOT09674.1"
                     /translation="MTTWHELLMRDHEVTERLFEAFGNALAREDGPGIKAVRMARLYF
                     SEYVDRCHNQKEEQHLFPLIEKRGIPRRGGPLAVMISEHGKSQTMLSTLVPLMEAYEQ
                     GDKSTLEPLRQAFAEYSTLLKNHFWKENDILYPMARKVLSEEDGASIVQGIEALEASF
                     GPDTRAKYYDMANQIEVHGGIEALSKSLDPDVLAAILDTLPVELSFVDENDVVRYFSH
                     EHLPKMFPRTRGVIGNTVQNCHPQKSVHLVNQILADFKAGKRDDAEFWIDMGGKKIHI
                     RYMPVRNQQGTYLGCLETVQDITHIQSLTGQKRLLDEE"
     gene            complement(11653..12033)
                     /locus_tag="PK710_07890"
     CDS             complement(11653..12033)
                     /locus_tag="PK710_07890"
                     /inference="COORDINATES: protein motif:HMM:NF023184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HOT09675.1"
                     /translation="MVETPYPAHFARELTTVRAMIGIYCRDHHGKHPGLCESCEQLWN
                     YVRERTERCPFGTEKPTCARCPVHCYQKEMRERIRQVMRYAGPRMIWRHPWLTLVHKW
                     HGRKEAPDLRTVRKRAGRRDTQGE"
BASE COUNT         2348 a         3348 c         3720 g         2692 t
ORIGIN      
        1 aaagtcatcg ccggcacgat tcccaacgga aaagtcttcg ttctcgaagg gcaaggtgcc
       61 gccaaagaac tcgtttcttt gccggatacc gaacacgtat gggccttggc cctcgacccc
      121 aaatccaaag ctgtatttgc cgccactggt ccggagggaa agctttttcg ccttggcgcc
      181 gatggcagcg cgcaagttca tttcgacagc gacgaagctc acctcatctc tctggtggcc
      241 gcgcccgatg gcaccttgta cgccggctcg agcggcaacg ctttgctcta caaaatcacc
      301 ggtcccggaa gggcaaccgt gatgtacgac tttcccggag acgatgtcaa agcgctggcg
      361 ctagccaaag acgggcgtct atttgcgatc gccaacgagc atcaaacgcc gcccatcatt
      421 ccacgaacga atcagtcgaa cgcggataaa tccgttggcg ccagcaagtc ggaccgtgtc
      481 gtgcccaaac ccggcaaggg tcgtttggtg cgttttgata cccacggtcg tgcggaggaa
      541 ttgcttttca acgcggattc acactttcag gcgttggcgc ttggcgacga cggcattcct
      601 tatgtgggaa cggccaaaga cggcgaagtc tatgcggtgg atgatgcgca tacatcgatg
      661 ttggtggcgg atacgccgga gcgccaggta ggggcgatgg tattggcggg gaacacgagg
      721 ttcgtggcaa ccaccgatcc cgccgtattt catccaatcc gtgcggtggg cggcacggaa
      781 tccgtgtgga atagcgcggt tctcgacgcc ggtttgcgtg ccacctttgg acagttgacg
      841 tggcgagcca ccggtccggt ggagttatcg acacgcacgg gtaacacgga aaaaccagat
      901 gcttcctggt ctccttggag tcccgcgatg gcggcgcctt ccaaaatggc ttctcctccg
      961 gcccgatatc ttcagatccg agcgcgcttc tctcgggatc cttccgccat agtccgggaa
     1021 atcaccgcgt atttcgtaac ggataacgcg cgcgccgtcg tgacttccgt gacggtgggc
     1081 gagcctggct cctcggacac caaaaaatca gactctgtcc ccgaatccgg cggcgacctt
     1141 gccgaagcaa agtccaaact caaactcagt tggaaagtca acaacccgga caacgactcg
     1201 cttcgtttcc gcctgtttta tcgattcgaa gacgccaccg tctggcggcc cattttgccc
     1261 gctcacgaaa tcctcaccaa aacgagctac gagtgggata ccgcagggct tccggaaggc
     1321 acctaccgag tcaaagtcga ggcttccgac gaactcgcca atccccccct ccaaaccctc
     1381 cggcattccc tcgaatcggg aactgtgctc atcgacaata cgccgccact tattcaacaa
     1441 cttacggtag ccggaaaaat catccgcggt gtggctgtcg atggggtggg ccccattgct
     1501 cgaatcgatg tcgccgtgga cccaaacctc tcgctttgga ctcccttttt ccccgccgat
     1561 gggatcttcg acgatcgttc cgagcgattc gaactcgacg tcagcagcct tttggcttcc
     1621 ggctcgggtc ttgtggccgt gcgtgtttcc gacgccgccg gcaatcgcgt ggtacgcacc
     1681 gttgatatcc gctgaccttc cccccctgac acataagtca gggtttccca ccaagcccct
     1741 ctagatttca agggattcgt gactggcacg acctttggat attttcacga ccgtgctaca
     1801 tttgaaattg gcctctctga tcgccatctg agcacagaag caagccagca tgcgtccaca
     1861 cacctcgatt ctctttctct tgtcgatgac ctttttggtc gcgtgtggtg aactcagttc
     1921 cctcgaggcg gcggaaacca acgccacctg ccccgacggt tgtttcaacg cggatgcggg
     1981 aacggatgcc cccgtatcgg atgtgaccgc ggacatcggt catgatgggg tgggcgagac
     2041 ggccacgctt tcgaatcccc tttgtggaac gctggtttgt gacccggatg atccaggtgc
     2101 gctcgactgt tatggctctg gccctggtat cgatgcgggt tcggggattg aattggatgc
     2161 ggcctatgag gcaggcgaga aggatgacga tcatgggggt ggaaagatgg ggggagcccc
     2221 gccggaaaaa ccgcatcctc ccccggataa tccccaagac aagccccagc gaatgtcttg
     2281 ccaggtggcc gccgatggct tcggacaacg ggaaaccacg tgtgttgccg cgggaaccgg
     2341 catcgataac gagccttgtg tttcttccgc cgactgcgcc gcaggacata cgtgcgtggg
     2401 ggacatcaac gtgggcgtgt gtcgcgccta ttgctgcctg aatgcggaat cgtgcgtgga
     2461 aggaacctat tgcgggatca tgcgttcgag agacgctttg accgcggatc cggaatccga
     2521 attgttgctt ccggtatgca ttcccgcgag cgattgtgag ttgcttcccg gtgccgaagg
     2581 aaacaaccgc tgcgccgaag gtttgatgtg ctcggtcgtg cgttcgaacg gcaccacggc
     2641 ttgtgttctt ccgggcaccg cggtggaggg tgaaccctgc ggaccctcgg attcgcaaaa
     2701 aagcccctgc gcggaaggat tcgtttgctc caaaaccacg aatacctgcc ttgccttatg
     2761 ccgcgtgggt gaccccaccg cctgtggaaa cggtgtttgc caaagcggaa cgggtggttt
     2821 gcccgaaccc tatgggattt gtgtcggaac ccgcagcgca ccgtgaaacg cgtttccacc
     2881 ctgcttttcg atgtcatggg gaccttggtc ttcgatcctt tccatgacca tgttcccgcc
     2941 tttttttcgc tcactttcga acagcttctc gagcaaaaac atccgaccgc ttggaaagaa
     3001 ttcgaacgag gctcgatcga cgaacccacg ttttttccca agttttttcg cgatggacga
     3061 agcttcgata ccaatgcctt caaggccatg atgaagcagt attacgcctg gattgatggg
     3121 atggaggcgt tggtgagcga gcttttcgac accggtgtgc cgatgcatgc gctatcgaat
     3181 tatcccccgt ggtattcgat gatcgaacaa cgaacggggc tgtcgcggtt cgtggcctgg
     3241 agcttcgttt cttgccatac ggggcttcga aaacccgatc cgaaagcgta cctgcacgcc
     3301 gcgcaagagc ttggccaacc gatggattca cttcttttca tcgatgatcg accgatcaat
     3361 tgcgatgccg ctcgatcggt gggcatgcaa gcgatcgatt ttgtcgatgc tcgccagctt
     3421 cgaaccgagt ttttcgaacg ggggttgctt gcggatcgtc aggtcaggcg tcgaccgtaa
     3481 cgacttcgaa gttggggggc tcgaggatag ttcgtttgac ggcgcataag tccgcggccc
     3541 gctgcactgg ttttttgtat ttctcgggga agtccggcgg aaggcgaatc tcgatgcgca
     3601 cgttttcgag tttatgggtg acggggttga agcccagacg ttgcaccaac gagatgcctt
     3661 cggtcggcag gttgcgagca cggcagaatt ccaggacata aatcccggcg caggttccaa
     3721 tcgaagccaa aaatagcgca taaggctccg gcgcagagcc gtctccgccc ccttctcgcg
     3781 cttgatccgt cgcgatcgtt cggcccaacc actccgcatt cactcgtttt ccaccggcaa
     3841 acgtgatgtg catctccatg cttatgttct cctgactgtt cggctcaccg taggagatgg
     3901 aactccaaaa agggattctc gaggtgcgtt tttcaaccct tgtatcccga atagggtggg
     3961 ctgtgagggc tggacagagc cgatgaagaa accggtggcg ggctgagcca tcccctcatt
     4021 ttatccgagt ctttccttca cgatcaccgt cccggtcacg gcccacgcaa accggtcacg
     4081 gtcacggtca cggtcacggc gcacggcaca caaaaccgat tccggtaatc ccatatcgtc
     4141 caccgcacaa cgccagtcca gtgtttggta gcccggggca actgttgagg gaactccaag
     4201 gggggcaagg gctgaaccaa aatcaacacc caagaatccc agctcgtatc cctttagaat
     4261 tcaggaaata atgaggggat ctgtcagcgt ggcgggggat tacttatccg acgggcaggg
     4321 ttgttcgcgc gcggcgaaag aaaacaccga gggtgcgatt tcttttggtt tgtggctttt
     4381 tttctgacga tcggcgaacc agaacaagaa cgccgggagc accaatacgg cagctagcgt
     4441 gcagatgact tcgcccactg cggcggcgag gccgaaactc acgatgccgc ggttgatgga
     4501 tagggtgagg gcgagatagc cgagggtcgt ggtcaacgcg cacaagatga ccgcgccgcc
     4561 tgtttccacc accacgcggt atacgcgttc cggaccttcg attcgataac gctgcatgag
     4621 gttatgcgcg tattcggcac cgatgccgat ggtgatgggg atggtcacga aactcaagaa
     4681 attgagcttg atattggcga agtaaaggaa agcgagcaat cccgccactc caccaagcca
     4741 gggcaaaaac acgccccagg cctgtcgtcg gaaacgaaac gcgagcaata tcaccagaat
     4801 cgatccggcc gcggataccg taatcgcctt gggagcatct tctcccaccg tttttatcat
     4861 gtccgcgaaa ataaccgcgc tgcccgaccc tttgatcacc tctcccgtgg gcaagggggt
     4921 ggaccgatag ctatccgccc agcgcatgag ataatgcgca tcccacacgc tgaatccttc
     4981 cttcggcaca atgaatacga tgcgccctcg cgtaccatcg cgttcggtga acggccgggc
     5041 gacttgctca ggaagactct ccaccgtgat gggttgcaag tcctccgcgg gaatatgagg
     5101 ctgcaaccgt tcccaatcgg cgtcgttgat gaatccacgg tctcgcgctc gttgaagacg
     5161 ctgtcgcatt tcgctcacga gcttgatttt ttcgggctgg tccttgggga tgagcgagaa
     5221 gatggatacg accttttcaa agggtttcaa gtcctccggc gcttctttcc atcgacgatt
     5281 gagttccact tccagcatgg gaacttgatc gagacggtcg gtcatgatgg ccatgccgtc
     5341 ctgtcccatt cgtcccacga ccttatcgac tcgcacggac aataggcccg cggaagtgcg
     5401 gtcctttcgc tcgttgcgta cgttggccat atcgtattcc atcggatctt cgatgagata
     5461 gagagccgcg gcgacgacgg ctaccgcgcc catgatggcg ccgacgatgg cgatgggccg
     5521 tggggcaaag cgggcgagtt tggcaaagag cacgccatag tatccgcgca tgaccgtggg
     5581 tttttcggtc tttcgaaacg aggggacgag acgttcgctg acggcgagaa tggcgggtaa
     5641 aaagagatag gtcgcgaacc agcacaagat catgccgtag ccactgatga cgccgaagtg
     5701 cttgaatccg cggaaatccg tgatggcgag cgaaccatag gcgagcatcg cggtggcgga
     5761 gccggcaagc gtgggaatcc acgagtcccg gtgggcgacc aggatggcgt ttttcggatc
     5821 ggcggcttca tcacgacgtg cttccaggta gcgcgccatg aacatgatgg aatagttgat
     5881 gccgtttccg gcgatgatgc tgaccagaaa acccgtggag gagttgaggt ggccgatggt
     5941 cagccgcgtg agaccgaagg tccacaatag ggcgatcatc acggtcatgc ccatggccgc
     6001 cacggttcga atccgcatga aaaacagcaa tacggcgcct aatacgccgg ccaggcccca
     6061 gcctcccacc tgcatgaggt caccgatgat cgcatcgtat tcctccgcgc tcgtgatgaa
     6121 attgcccgtg tagttcacct ccatcgatgg gtcgaaacgg cttggattca ccgaggccac
     6181 gacattctcc acctcggatc gaaactcggc cttggcctcc ttgcccgaca cggatgtgcg
     6241 aatcaaaacc gcgatgaagt tgccttccgg gttcatatag tagccgtcgc gataccggcc
     6301 ggtcaccgcg tctgtggcag aaccacttga ttttccacgg attttttctt cgatgctatc
     6361 cgcggtcagg ggcgtgggaa cgtcgtcgtc gtcgatgaag tcaccgcgcg ctttggcgac
     6421 ctcgtagtcg tagcgtgaaa tgagttcgtc gtgggctttt tggatatctt cgagttgcgc
     6481 gtacagaacc ttgttgtggt cgaaaaattc ctgggttttt ttcacgccga agtcgacggc
     6541 gcccactttg tcaggcccga gcgcttcgag tttgggcacg agcgcatcga cgaatcgttt
     6601 gagcgcctcg gggtttggat gcgcgcgggt ttctgccaca acggtcaggg tcgatgcgcc
     6661 ggccaatcgc tcaccgaccc ttcgtgcctc gatgacgctg tctttattgt cgggaagcag
     6721 ttcgtcgaag gaggacttga agccgagccc ccccgcgcca aaaatgccgt atagcgcggg
     6781 caacaagctg agaaaggcga tggcgaggaa gcgccacgga tggtcggctt ggagctgtcc
     6841 aaggcgtcga aggatagggg gaatattgcg ttcggacatg ttgatcgatc aagcaaacag
     6901 caaacttcgt gcccagcaac ttacggcaac caaaagaaat catccctcgg tggatgcggc
     6961 gtgaccatga tgcttcccgt tcctgatttc gaaaaccaag aacttacgaa cacagtgtga
     7021 atcctgcgct caccccggta acgagtcggc gcagccagac tttcgcttgc gtctcccgtt
     7081 gacagccggg ggcagggagc ctcttattca tgccacgtcg acgccgcgag gccaaccgga
     7141 agtcgtcata ccaggtttcg tggaaaaata gcccgccatg ggtggctgaa cgcgacgttc
     7201 gtggactggg aggagcagca tggcaaaatc gcgcagacga agcatctacc cgatattgct
     7261 cattattttg gcaaatggcc tggtgttttc cggctcgatg gttccccttg cgtattgggg
     7321 gttcgaagac ggtcctcctg tggatgtggc ggcggcgggc tttgccggtt ggttgatcat
     7381 ggcgctttcg gtgctggggt tggccatcat gattttgggt ggcgtgctgt tgcgggccgt
     7441 tccccggatt cctgctttcc tgctcggtct tttccccgcg tttgtcgccg gcgtcggtgt
     7501 gttgggagcg caaatcggtc ttgggaacgt cttcgaggct gtcggagtcg cgggcgcgga
     7561 caggatccaa gtcgctgccc tcggcgtttt tgaagccctc aacgcgtcgg tcatcggtgc
     7621 gtatgtggcc gctgccttga tgatggcgag cgcgttgacc atctccttgc gtcactggcc
     7681 ttcccgcggg tcgctgagtt ccggcggaaa agtcggcttg gtcggtggtg ctttcctgct
     7741 aatcgccatg atcatcggct cgcttttctg gactccgctc ggaggactcg gtgtgttgcc
     7801 ctggattacc gccttcgccg gtttggtagc catcgcggtc gcggctcacg ccattcgcaa
     7861 tgaccccacc gatgccgcca atgtgcaagc cgccggagag ttgtgggtaa ctctgttgct
     7921 atcgatgggc gccatcgtat gggtcgtcac ggcacggcat acgagcgctt tgatggaagg
     7981 ctccatgacg ctctcggcct cttcgcccca tctcgatcct tcaaaggtga tcgagtcgtg
     8041 gaccgccgcg gggcccgccc tgtacgccag tgcgctctat gtcgtgccct tgttattcgc
     8101 ggccgtgatg tcgatgctca gccgagaatc actcggttcc tgggggcttc gcaaggcagc
     8161 ttcgagtttg cttttcgttc ctctgttttt tgcggtgccc gcggtgctgc agcatctgca
     8221 aaccggatcg gctgcgaaac gattggctga cgttctttcc tgcgcgcctg cctggttgcc
     8281 gcgcgttcaa caaaccctgc ccatcacgaa aaacgccatg gagtcatcgt gcccggaggc
     8341 ggtgctggaa atcggacctt cggaaatacg gcttggagac aaaaacctcg gggcgttgtc
     8401 gaagctcgat tcctccgacg ggtgcgcggc ggtgtcgtcg caactagtcg aatttcaagg
     8461 attttttgcc gtgtcgagca ccctttccta tcgtcgggcc gcctgtttga tcgatgcgtt
     8521 gagccagtcg ccttctttcc ggggccgttc cgatatgtat cgtcagcgca atccggaatc
     8581 gtctgcggga agagcgggag gcgtggtttt ttggttgctg caatcggaac cgcggggaga
     8641 agcatcggcg cggccaccgt tcgatcaatt gggaaccagg ttgtggcagc ttacgacctt
     8701 cggtccttcg gcggaagggt gggacccccg gctggtgcat ctgcatgtca tggaaaatgg
     8761 gtggacgttg aagcgagatc cggcgtcgac gccgatgcgt ttcgaagggt ctgccgtcga
     8821 tggattgagc aaactccaga gcgtccttgg tttgaatatg gccgcgcagg taatggtcct
     8881 cagtgccgaa ggcgccgtga gcatgggaca attgctgcac tatgcgagtg cattccgcga
     8941 tccccagctc atgatgcccg cggtcgaacc gtccgacaac gcattgcctg acgacaagga
     9001 cgccggggta ggggatgggg atagtcccga ggccatcgcg cccgaggcgg ctgccacgcc
     9061 tgcggcttcg gcatcgagcg cgggttcggc gtcatctgcc gttggccccg accgatgact
     9121 ccgatacgaa ggattcatcg gttccggcgc atcacgacga aaggattcca ttcatgaaaa
     9181 cgctcatcat tttgaacgat ccgccttatg gtacggagcg aacgtataac ggtcttcgtc
     9241 ttgcggcgaa tatgctgaag atggacgagc aagagccgga gatggtggtt tttctcatgg
     9301 gggatgccgt cagttcggcc aaaaaagggc agaaaacacc gaacggctac tacaacctcg
     9361 agcgcatgat cgaactcgtg ctgaagcgtg gagctgaagt atggctttgt ggaacgtgca
     9421 tggatgcgcg gggcattggg gaggatgaag tcgtgcaagg ggcgcggcgc agcacgctcg
     9481 atgcgttgac ccaggccacc ctggaagcgc aaaaagtcct ggtgttttga tggcttccat
     9541 ctatctggac cataacgcga ccacgccttt gcatcccgat gtcgtggaag cgatgctgcc
     9601 gtatctcacg acgcacttcg gcaatccttc gagtggtcat gaggttgggt tgcgggccag
     9661 ggcggcggtg gaagcggcgc gggaacaggt ggcctcgctg ctgggatgct catcggaaga
     9721 agtggttttc acatctggag gcacggaatc caacaatctc gccctgcggg ggctgacggc
     9781 gacacatgct ggcgccatgc atgtcttgac ctccgcggtc gagcatccgg ccatcgagat
     9841 gccttgccgg cagttggagc ggcaaaacgt gcgtgtgacg cgtgtggccg tcgattcttt
     9901 cggtcgggtc gatccgggtg cggtcgtgca agcgttggaa cgagatacgg tgttggtgac
     9961 ggtgatgctt gcgaacaacg agacggggac gttgatgggt gtgcgtgaga tcgcgcatga
    10021 ggcgcgggcg cgcggtgtca tcgttcatac ggatgccgcg caagcggtgg gcaaggtgcc
    10081 gacgcgggtt gcggatctcg gtgtggacat gctatccata gcgggacata agttgtatgc
    10141 gcccaagggg gtaggggcgt tgtatcttcg caagggaatc aagatttcac cgctattgtt
    10201 cggcgcgggg catgaacggg gcatgcggcc gggaacggaa aatgtggcca gtatcgtggg
    10261 gctgggagcc gcctgcgcgc ttgccgaacg cgatggcgcg cggcaagccg atcgtcagag
    10321 gggccttcgg gacaagctgt gggagttgct gaaagagaag attcccggtc tatccctaaa
    10381 tggacaccca agcgagcgtc ttcccaatac gttgaatgta tcttttcccg gggtgaaggg
    10441 gagtgaggtg ttggcgcggg cccaggatgt ggcggcgtcc acggggtccg cctgtcatgc
    10501 gggaagcgaa acgccctcga cgattctcaa ggccatgggg atatcggatg cggtggcgct
    10561 cggcgccgtt cgtctttcgc tcgggcgtga aacgagcgaa gacgacattg tccgtgcggc
    10621 cgcgagcttg gttcacgcct acgatgccgc cgcgaaggcg tgatcgcgcg cgtgttttca
    10681 cgcggaaaat aagctgattt tgtgattatt cctcgtcgag aagacgtttt tgcccggtca
    10741 gggactgaat atgggtgatg tcctgcacgg tttcgaggca gcccagatag gtgccttgct
    10801 ggttgcgaac gggcatgtag cggatgtgga ttttctttcc tcccatgtcg atccagaact
    10861 cggcgtcgtc gcgcttgccg gccttgaagt cggcgaggat ttgattgacc aggtgtacgc
    10921 ttttttgcgg atggcagttt tgcaccgtgt tgccgatgac gccgcgggtg cgagggaaca
    10981 ttttcggaag gtgttcgtgg gagaagtagc gaacgacatc gttttcgtcc acgaacgata
    11041 gttcgacggg aagggtgtcg aggatggcgg cgagcacgtc gggatcgagg gatttggaca
    11101 acgcttcgat gccgccgtgg acctcgattt ggttggccat atcgtaatac ttggctcggg
    11161 tgtcggggcc gaagctggct tccaacgcct cgatgccttg cacgatgctg gcgccatcct
    11221 cttcggacag gacttttcgc gccatcggat acaggatgtc gttctctttc cagaagtggt
    11281 ttttcaataa cgtgctgtat tcggcgaacg cttggcgaag cggctcgagg gtggatttgt
    11341 caccttgttc gtaagcctcc atcaggggca ccagggtgga gagcatggtc tggcttttgc
    11401 cgtgttcgct gatcatgacg gcgagggggc cgccgcgacg gggaatgccg cgtttttcga
    11461 tgagggggaa gaggtgctgc tcttcttttt ggttgtgaca acgatccacg tattcggaaa
    11521 agtagagcct tgccattcga acggctttga taccgggccc atcttcccgt gccaacgcgt
    11581 tgccgaaggc ttcgaaaagc cgctcggtga cctcgtggtc gcgcatcaac aactcgtgcc
    11641 atgtcgtcat gttcactctc cttgtgtatc tcgtcgaccc gcgcgttttc gcacggtccg
    11701 taaatccggt gcttcttttc tgccgtgcca tttatgcacg agggtgagcc agggatggcg
    11761 ccatatcatg cgaggtccag cgtagcgcat cacctgacga atccgctctc tcatctcttt
    11821 ttggtagcaa tgcacggggc atcttgcgca agtcggtttt tcggtgccga atgggcatcg
    11881 ttcggtgcgt tcgcgaacgt agttccagag ctgttcgcag gattcgcaaa ggcccgggtg
    11941 ttttccgtga tgatcgcggc agtaaattcc gatcatcgcg cgaacggtgg tgagttcgcg
    12001 ggcaaaatga gcggggtaag gcgtttccac catggcgggg gttgtagggc tttgggggga
    12061 ttaaggctat gacggatgtc agttgggggg gggggtttcc cccatcct
//
LOCUS       DAOSOR010000106        11987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5288_length_11987_cov_4.398676, whole genome shotgun sequence.
ACCESSION   DAOSOR010000106 DAOSOR010000000
VERSION     DAOSOR010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11987
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5288_length_11987_cov_4.398676"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PK710_07895"
     CDS             complement(<1..328)
                     /locus_tag="PK710_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09676.1"
                     /translation="MTPARRSWRILTLLLTIAACQAEEAKTSSPEDDADNAKLVTGRS
                     PSISSTSSSTSLPSPPPEASKKSCPVGMVRIEGGVFRMGAPYAVHPQNDAYLHEVALR
                     SFCLDRH"
     gene            complement(355..1071)
                     /locus_tag="PK710_07900"
     CDS             complement(355..1071)
                     /locus_tag="PK710_07900"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT09677.1"
                     /translation="MFWPVKRWLCPMGLALWALPQVANAETVTDTHAEPLRPKKQYIV
                     AAIGDSLTDARGGGGKYLSYLRKRCPGSQFDNYGRGGEMVNQMRRRFMQDILGPAKPT
                     YTHVIVFGGVNDVYSDQTAKRTPAKIQADLLAMYTMAKEAGIHVVALTIAPWGGFRRY
                     FNPTRASATRQVNRWIDQQQQEGVIASAIDAYGLLSCGDPEKLCPDFEPPFRDGLHFN
                     EKGHERLGEALWNRVFSDCR"
     gene            1192..1566
                     /locus_tag="PK710_07905"
     CDS             1192..1566
                     /locus_tag="PK710_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09678.1"
                     /translation="MQIFHERRRQRSDSKREALMYQLQACARRAGIEAMVLADEQGRV
                     IARSDRLPIAQEVAVFGPFLAKPKTWFGKVQLDGQSRSLAVSPFRLGTSMAYLCAVGA
                     KRHNVGGAFLQAAAGVRRIMTP"
     gene            complement(1730..3100)
                     /locus_tag="PK710_07910"
     CDS             complement(1730..3100)
                     /locus_tag="PK710_07910"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF005301.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOT09679.1"
                     /translation="MLTPERLEVDTLGECTVRSPLNLSTIPGDEIADFVPDNARVLYQ
                     VERRAGEPIDTILDFEKAGPREKLYFEPRNTRAAIVTCGGLCPGLNNVIRSIVLQLHH
                     NYHVKNILGYRYGYQGLNPEHGCPPMELGPDQVRTIHSRGGSFLGMSRGNENIATMVD
                     TLVRDDVQILFTIGGDGTLRGATALYEEIQRRGLNIAIVGVPKTIDNDISYVDKTFGF
                     DTAVEVARLALDGAHTEAISAQNGIGLVKLMGRDSGFIAAAATLASLDVNYCLVPEVH
                     FDLEGPQGLLTHLQHRLSKRGHALIVVAEGCARAFASQPLTHDASGNVQYGGPDLDVG
                     LYLKDKITSYFKAAKIPVTLKYIDPSYMIRSVPANANDSIFCDILARHAVHAGMAGKT
                     GLVIGRWHNVFIHAPLVLATSKKKTINPDSELWLAVTETTGQPDLSNIPVSRRAAAWR
                     EAQKQR"
     gene            3240..4268
                     /locus_tag="PK710_07915"
     CDS             3240..4268
                     /locus_tag="PK710_07915"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOT09680.1"
                     /translation="MIALTMVANTKFRIYSGFSTDVGTTRERNDDTLVQRKIPLGYFA
                     AVADGMGGHADGDTAAALTVSSMLEEVEKASNDPSEALRNGFVAANTAVRRRCQDAGV
                     IMGATCAAAIISAGQLYIGHVGDSRVYLLRGANLYSLTRDHSLMQEIADIKGPVAAGG
                     FAPTLKHIMSRSVGAEDDIQPAIRTPVPLATGDAILLCTDGLTNTLDDRVIRRVLAGG
                     TPREAAYRLVDLTNASGGEDNVTVIVLRVDAADAQEQSSVMSLDELLSLSVQTSDGDL
                     HPIIDVTMNPMNWSVVGLKLDLRNVERGATCEIPVSEMGPLAATDGVITTPQRTEALL
                     DISHSSKE"
     gene            complement(4279..5244)
                     /locus_tag="PK710_07920"
     CDS             complement(4279..5244)
                     /locus_tag="PK710_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOT09681.1"
                     /translation="MTIWPVFGEVDSQPLHRSMSCWHSSLSPSAIALYGRRMVVTEDR
                     VLRAMTDDASFRVMTAITTNTVAEVMKRQNPPSDEMARLGDLLTGTILIRETMSPNLR
                     VQGILRGAGGKGTLVADSHPDGTARGLVSLPQGMTSLPAGSGATLMMMRTLPNGKLHQ
                     GMVSYDSNANVSEALMRYMQESEQIKTMIDVACVVEQEQVVAAGGFIVQLLPELTESQ
                     LAIMTERLSDFPPMGKLLRNEATASQVLDELLYAMPNTRLGDSPLRFDCKCSQTRVVA
                     SLATLDRNELGNLLTDGHPIEICCDFCGKEYAITAEQVRGLLRES"
     gene            5250..6170
                     /locus_tag="PK710_07925"
     CDS             5250..6170
                     /locus_tag="PK710_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09682.1"
                     /translation="MTVTFNRRRMLASLAGLCTASRGSRAEAALALSEHELIDLRLPG
                     QAQLGNRITVYTPKHTRGGTLPVLVLLHGLGETGNDRDGAYAWAERYGLLTSYERLLH
                     PPVDQGLRHHRFLPKIRAEAIDRELMLYPFRGMILVCPYTPNVFQMNTMRALDDYAAW
                     LVEIVLPEVHSRTPSKQGLRFTALDGCSLGGFISYQVFLRRPEAFYCCGGVQAAIGKD
                     TAAFYAQQFHDIVQRLGPRRFHLETSTQDPYHDANVQLARQLERRGVEHDLRVLPGPH
                     NQPWLIEAGTLEMLLWHDRQLAMPLVSTGT"
     gene            6229..7245
                     /locus_tag="PK710_07930"
     CDS             6229..7245
                     /locus_tag="PK710_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09683.1"
                     /translation="MGSVQRLGWTLGFMAACAAFSPDVSAAGAALHQATGAQKQEAQK
                     LFLNAKQSFDKQQYDAALTGFLASYDVVASPNSLLMAARALVALDRLEEAFATYQEVI
                     EVAKEAAAKDKKYAPSLESAEKEFEELRPRIALVTFDIVGATSDTELFINDQPMARDR
                     WDKQIPVRAGVVSITAKSPGKPDFQQDLTLSGGASSQRIDLQAFWAPPPPPPSTDIDD
                     ESSAHGNVDLLGLDKRTWAYIAGGVGAAGVVTFGVFGALNQSKYNNLQDDCPNGRCPS
                     DRSDDINAGRRYQTIANVGLVVGVVGLGTGTALYFLSKDSGREQPTTQVGFGPGSVTV
                     QGTF"
     gene            7245..>7455
                     /locus_tag="PK710_07935"
     CDS             7245..>7455
                     /locus_tag="PK710_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09684.1"
                     /translation="MNKKLVCLVVPLSMWFLGCTNDYDEFSFQPGSTDGGADSSKGGS
                     GGATGGSGGATGGSGGATGGSGGATG"
     assembly_gap    7456..7555
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7556..8451
                     /locus_tag="PK710_07940"
     CDS             <7556..8451
                     /locus_tag="PK710_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09685.1"
                     /translation="GSGGATGGSGGATGGSGGETGGSGGATGGSGGATGGSGGAAGCG
                     GTQKLCGSTCVDLDDPQTGCADPNSCDPCVIPNATAACELGQCAIGTCNTGFEDCDHD
                     TATGCDAELAVSTDHCGACARACSDAHVVTPVCEKGLCMSTCETGYGNCEQPATGDDD
                     GCETNLKDSTDHCGGCNNACPSNAAQHFVCRDSRCACDSNEDCSIGGATGTCYLSNPS
                     DYRYGRCRCGNNDCRPGEVCHTVNSQSRCSCNGGQGCSTSQTCCQTPAGCFYLNWDRN
                     NCGACGHVCPTGQNCIDGSCH"
     gene            complement(8465..8641)
                     /locus_tag="PK710_07945"
     CDS             complement(8465..8641)
                     /locus_tag="PK710_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09686.1"
                     /translation="MPVCKECGDEVDELVRVKVGGRTLKLCEDCADRAREQDEIAEQS
                     ESVVQGMMGFKGRR"
     gene            complement(8731..9501)
                     /locus_tag="PK710_07950"
     CDS             complement(8731..9501)
                     /locus_tag="PK710_07950"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT09687.1"
                     /translation="MARRLVSHVRVSYRASGLALLTVAQLGAVELHQRLASGDHADGI
                     FDRHMRLWVRTLLRLFGVHVRQSPALAPRKGAAARMIVANHRSPIDIAVLLSLFGGQI
                     LSRADLSQWPILGRAARKAKTIFVDRDQSASGATAIRQIRRSLQQGATITVFPEGTTF
                     AGDEVRPFRKGAFVGANGLDVEFVPVGLAYEPGTEYVDETFVAHLQRSAARKRTNVAV
                     CMGTPRTTKGRAAELAPLLQQDVQNLVVEARKLLSTRG"
     gene            9628..10869
                     /locus_tag="PK710_07955"
     CDS             9628..10869
                     /locus_tag="PK710_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09688.1"
                     /translation="MSFHLSTVMVFFLLLMGCQPSSPPQAAAPPLTPPLTTTPPPVAT
                     PPTMPTADSQPPVPEPESAEPTMRPLEIPFAQLVQGISEKDAYYFSDNYVSNETAFLQ
                     VAPELPKAVAPGGAFLGVGPEQNFSYIALTRPDIAFVIDIRRRNMLLHLLYKAIFEEA
                     SSRSHFLALLLGRNHDTSTDPGDGASIEDVLAHATKVAKSREAFQTIHQRLVDRIAHD
                     YGITLDFGDRNALRESHREFFDKQLEIAFELHMKSARKYPTLRTLLTMRDPSGTQTSF
                     LADEKAFRLVQKMQKENRIIPVVGDFGGDKALLGIAEFLRANQIRLSTFYTSNVEEYL
                     LQDHKWDKWIRNLDAFAIDDRSVFIRSYLARAGRTHPLQLEGHWSTTFLQRIQLFRDR
                     EREHQFRQWHDVATYELMTPP"
     gene            complement(10944..>11987)
                     /locus_tag="PK710_07960"
     CDS             complement(10944..>11987)
                     /locus_tag="PK710_07960"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT09689.1"
                     /translation="ALRRANSPDEMRRVRAALLLDEGYPVDIFEKQYSAATSDLSRLE
                     IVRRYLTLAPHDVRLQRLMLTLLESTGGKDALRQEIARIRRDPFSDATLLADAATALR
                     SLGHEAEALRTFSEIVERAPRDPWARAYTGDRLRREGWYDAAVSMYAPLERAMPFDQP
                     VLMRLALAHAGANRIDLAGRMLTRLTQNGGRSTTSDLSDLASDLATVLLLTPHEGISK
                     PQQDELTRRAMELPRRTSGTVVLVRSASASPALSAILRRGPKEAKEERKPDISAEAID
                     MLRLVMDPGDNDVVLQLTAEEALPPSLPIKVQVDAIVSQGPTQAPVLVTKEVQVPRTG
                     KPVDMVWDGGAWK"
BASE COUNT         2526 a         3443 c         3358 g         2560 t
ORIGIN      
        1 cgtgccgatc gagacaaaac gatcggagtg ccacctcatg aaggtaggca tcgttttgcg
       61 gatggaccgc ataaggggct cccatacgaa aaacaccccc ttcgatgcgt accatgccga
      121 cagggcaact ttttttcgac gcttcggggg ggggggaggg cagagaggtg gatgagctgg
      181 tggaggaaat ggaaggggat cggccggtca cgagcttcgc gttgtcggcg tcatcctcgg
      241 ggctcgaggt tttcgcctct tcggcttggc aagcggcgat tgtcagcaac aaggtgagta
      301 tccgccacga acgacgcgct ggggtcatgc taccgtcacc gtttccctgg gtatttatcg
      361 gcaatccgaa aacacgcggt tccagagcgc ttctcccagc cgctcatgcc ctttttcgtt
      421 gaaatgcaag ccatcgcgga aaggaggctc gaagtccggg cagagttttt ccggatctcc
      481 gcaggataac aagccatagg catcaatggc gcttgcaatg actccctctt gttgttgctg
      541 gtcaatccat cgattgacct gtcgcgtggc cgatgctcgg gttggattga aatagcgacg
      601 gaaccccccc catggtgcaa tggtcaaggc cacgacatga atgcccgcct ctttcgccat
      661 cgtatacatg gccaataaat cggcttggat cttggcgggt gttcgcttgg ccgtctgatc
      721 gctgtacaca tcattgaccc ccccaaatac gatcacatgc gtataggtcg gcttggccgg
      781 cccaagaata tcttgcataa aacgtcggcg catctgattg accatttcac cgccgcgacc
      841 gtagttgtcg aattgactcc ccgggcagcg ttttcgtaaa taagacaagt acttaccccc
      901 accaccgcgc gcatcggtca acgaatcccc tatcgccgcc acgatatatt gctttttcgg
      961 acgcaacggt tcggcatggg tatccgttac cgtttcggcg ttcgccacct ggggtaacgc
     1021 ccatagagcc agccccatcg ggcaaagcca tcgttttacc ggccaaaaca tgacggcaac
     1081 cgtagcatgt ctccgaacgc ctcacagcgc ccggaaggaa agaaccgaga attttgcggt
     1141 ccggaagaga gttgatacta tgtaggacaa atggctacaa acaacaaaaa catgcagata
     1201 ttccacgaac gacggcgaca acgcagtgat tccaagcgtg aagccctgat gtaccaattg
     1261 caagcttgcg cgcgtcgcgc gggaatcgaa gcgatggtgc ttgccgacga acaggggcgg
     1321 gtcatcgcgc gcagcgaccg gcttccgatt gcgcaagagg tggccgtgtt cggacctttt
     1381 cttgccaaac ccaaaacttg gttcggaaag gttcagctcg acggtcaatc gcggtctttg
     1441 gcggtgagcc cctttcggct tggcacatcg atggcctatc tttgtgccgt aggagcgaaa
     1501 cgccacaacg tgggaggggc atttcttcaa gcggcggcag gggtacgacg catcatgaca
     1561 ccgtaatggg gtgcttgcgc gggaagagtc cttggggccc gcgatcgcat ttggggggtg
     1621 gaacggctaa ataatacaaa taaatcaatt ggttgcgtgg atcttgccaa gttctgcgga
     1681 aaagacgttc cgcttgcaac gaggggtgaa cgtatggggg gtggtgaagc tagcgttgtt
     1741 tttgggcttc gcgccaagcg gcggcgcgac ggctcacggg gatgttggat aggtcgggtt
     1801 gcccggtggt ttcggtgacc gccagccaca attcgctatc tgggttgatg gttttctttt
     1861 tgctggtggc gagcaccaac ggggcgtgga tgaacacatt atgccagcgt ccgatcacga
     1921 gacccgtttt cccggccatt ccggcatgga cggcgtgacg tgcgaggata tcgcagaaaa
     1981 tgctgtcgtt ggcgttggct ggcacgcttc gaatcatata gctcggatcg atatatttga
     2041 gggtgacggg gatcttggct gctttgaagt acgacgtgat tttgtctttc agatacaatc
     2101 cgacatcgag atccggaccc ccatattgga cgttgccgga agcgtcgtgt gtcaagggtt
     2161 gggaggcgaa agcgcgtgcg cagccttccg ccacgacgat cagggcgtgg cctcgtttgg
     2221 acagacggtg ttgcaagtgc gtgagaagac cttgcgggcc ctcgaggtcg aagtggactt
     2281 ctggaacgag gcaatagttg acgtcgaggc ttgccagggt tgcggcggcg gcaatgaagc
     2341 cggaatctcg acccatcagc ttgacgagcc caatgccatt ttgcgcgctg atcgcctcgg
     2401 tatgggcgcc atcgagagcg aggcgcgcca cttcgaccgc ggtatcgaat ccgaaggttt
     2461 tgtcgacata agagatatcg ttgtcgatgg ttttgggtac gccgacgatc gcaatgttca
     2521 ggccgcgtcg ctggatctcc tcgtacaggg ccgtagctcc gcgtagggta ccgtctcctc
     2581 cgatggtaaa cagaatttgg acgtcgtcgc gaacgagggt gtcgaccatc gtggcgatgt
     2641 tttcgtttcc ccgggacatg ccgagaaagc tgccgccccg cgagtggatg gtacggactt
     2701 gatcgggccc gagttccatc ggagggcagc cgtgctctgg gttgaggccc tgataaccgt
     2761 aacggtatcc gaggatattt ttcacgtggt agttgtggtg gagttgcaga acgatggatc
     2821 ggatgacgtt gttgaggccg gggcataagc cgccgcaggt gacaatcgcg gctcgggtat
     2881 tccggggttc gaaatagagt ttctctcgtg gcccagcctt ttcgaagtca aggatggtgt
     2941 cgatgggttc gccagcgcgt cgctccacct gatacaaaac ccgtgcgttg tccggtacga
     3001 agtcagctat ctcgtcgcct gggatcgtgg ataggttcag gggagagcgg accgtacatt
     3061 cgcctagcgt atcgacttcg agcctctctg gcgtgagcat gcagtacctc gctggggtcg
     3121 gggcgttgct ttcgacaacg gatggaatgt cgccaattgc tctccgaact tcaacggctt
     3181 cgatacatga tcttggactt tgtcatcaga tgcgttgacg caagcctcga gtcgctttca
     3241 tgattgccct tacgatggtc gcgaacacca agtttcgaat ctattctggc ttcagcacgg
     3301 atgtgggcac cacccgcgag cgaaacgatg atacgctcgt gcagcgaaag atccccctag
     3361 gatactttgc cgctgtcgcg gatggaatgg ggggacatgc ggatggtgat accgcggccg
     3421 cccttacggt cagctccatg ctggaggagg tcgaaaaggc cagcaacgac ccatccgaag
     3481 cgctacgaaa tggttttgtg gccgcgaata ccgcagtgag acgtcgatgt caggacgcgg
     3541 gtgtcatcat gggtgccacg tgtgctgctg ccatcatttc cgctgggcaa ctttatatcg
     3601 gccatgtcgg tgattcacgg gtttatttgt tgcggggagc caatctgtat agcttgaccc
     3661 gggaccactc actcatgcag gaaatagctg atatcaaagg tcctgtcgcc gccgggggct
     3721 tcgctcccac cctcaaacac atcatgtccc gttcggtggg cgccgaagac gatattcaac
     3781 ccgctatccg cacacctgta cctctcgcca ccggcgatgc cattttgctg tgtaccgatg
     3841 ggctcaccaa cacattggat gatcgggtca ttcgtcgcgt gttggctgga ggaacaccac
     3901 gagaggcagc ctatcggctc gtcgatctca ccaatgcttc cggtggagaa gacaacgtga
     3961 ccgtcatcgt gctgcgtgtc gatgccgccg atgcgcaaga acaatcctcg gtcatgagtc
     4021 tggatgaact tctatcctta tccgtacaga catccgacgg tgatcttcac cccatcatcg
     4081 atgtgaccat gaatccgatg aattggtcgg tggtgggatt gaagctcgat cttcgcaatg
     4141 tggagcgtgg tgccacttgt gagattcccg tcagtgagat ggggcctctt gcagctaccg
     4201 atggggtgat caccacaccg cagagaacgg aagcccttct ggacatttct cattcatcca
     4261 aggaataaag cgttcgtttc agctttcgcg cagcaagcca cgcacctgct cggctgtgat
     4321 cgcatactcc tttccacaaa agtcgcagca aatctcgata gggtggccat cggtcagcaa
     4381 gttgccgagt tcatttcgat cgagtgtggc caaactcgcc acgactcgtg tttgactgca
     4441 cttgcaatcg aatcgcaagg gagagtctcc aagccgtgta ttaggcatgg cgtacagcaa
     4501 ttcgtcgagt acctgcgacg ccgtcgcttc gtttcgcaac aacttgccca tcggtgggaa
     4561 gtccgatagc cgttcggtca tgatggcaag ctgcgattca gtgagttcag gcaaaagctg
     4621 aacgatgaat cctcccgccg cgacgacctg ctcttgttcc acgacacaag ccacgtcgat
     4681 catcgttttg atttgctcgg attcctgcat gtacctcatc aacgcttcgg aaacgttggc
     4741 gttggaatcg taagacacca tcccttgatg gagttttccg ttgggcaacg ttcgcatcat
     4801 catcaaggtt gcgcccgatc cagcaggcaa agaagtcatt ccctgtggca aactcacaag
     4861 accgcgcgcg gtaccatcgg gatgcgaatc ggccacgagc gtacctttgc cccccgcccc
     4921 tcgaaggata ccttggaccc ttaggttcgg tgacatcgtc tcgcgaatga ggatggttcc
     4981 cgtgagcagg tcaccaagac gggccatttc gtcggaaggc ggattttgcc gtttcatcac
     5041 ttccgccacg gtatttgtgg tgatcgctgt catgacccga aacgatgcat catcggtcat
     5101 ggcgcgcaat actcggtctt ctgtgacaac catgcgccgt ccatagagcg caatggccga
     5161 tggtgacaac gaagaatgcc agcaagacat cgaacggtgg agcggttggc tgtcgacttc
     5221 gccgaatacc ggccatattg tcatgaacca tgaccgtcac gttcaatcgc cgtaggatgc
     5281 tggcctcgct agcgggactg tgtaccgcaa gccgcggtag tcgcgcggaa gcggccttgg
     5341 ctctctccga gcacgaactc atcgacttgc gattgcccgg tcaagcccaa ctcggaaatc
     5401 gaatcacggt gtatacgcca aaacacaccc gcgggggcac cttgccggtg ctggtgctcc
     5461 ttcacggact cggcgaaaca ggaaacgatc gcgatggtgc ttacgcctgg gcagagcgtt
     5521 atggcctgtt gaccagctac gaaagactgc ttcacccgcc tgtcgatcaa ggactgcgcc
     5581 atcaccgatt cctgccaaaa ataagagccg aggcgatcga tcgtgaactt atgttgtatc
     5641 ccttccgggg catgatcctc gtttgcccct ataccccaaa cgtttttcaa atgaacacga
     5701 tgcgggcgct cgacgattac gcggcgtggc tcgtggaaat cgtacttccc gaggttcatt
     5761 cccgaacccc ttcgaaacaa gggttgcgat tcaccgccct ggatggttgt tcgctaggtg
     5821 ggttcatctc ctaccaagtg tttttgcgtc gaccagaagc gttttattgc tgtggaggcg
     5881 tgcaagcagc gatcggaaaa gacaccgccg ctttctacgc gcagcagttt cacgatatcg
     5941 tgcagcgtct cggtccacga cgttttcacc tggaaacaag cacccaggat ccgtatcacg
     6001 atgccaacgt gcagcttgcc cgacaactcg aacgacgtgg tgtcgagcat gaccttcggg
     6061 tgcttccggg accccacaac cagccctggc tcatcgaggc cggaaccctc gaaatgctgc
     6121 tttggcacga tcggcaactt gccatgcccc tcgtgtcgac cggcacctga tacgaattca
     6181 ctctccgtgg atagtcgttg cgatggcgaa tgtggtactc tcccccgcat gggatcagtt
     6241 caacgacttg gatggacgct tggtttcatg gcggcgtgtg cggccttcag cccggatgta
     6301 tccgcggcgg gcgctgctct ccaccaagcc accggtgcac aaaaacaaga agcccaaaag
     6361 ctctttctca acgccaaaca aagcttcgac aaacaacaat acgatgcggc cctcaccggg
     6421 tttctcgcgt cctacgacgt tgtcgcaagc cccaactccc tgttgatggc tgctcgagcc
     6481 ctcgtcgctc tcgatcggtt ggaagaagcc ttcgccacct atcaggaagt catcgaagtg
     6541 gcgaaggaag cggcggccaa agataaaaaa tatgccccct ccctcgaatc cgcggaaaaa
     6601 gaattcgagg aactgcgtcc gcgcatcgcc ctcgtcacct tcgatatcgt tggtgccacc
     6661 tccgatacgg aattgttcat caacgaccag cccatggcac gggatcgatg ggataaacag
     6721 atacccgtgc gagcgggtgt cgtgtccatt accgcaaaat ccccaggaaa acccgatttt
     6781 caacaggatc tgaccctcag cggtggagcg tcttcccagc gtatcgactt gcaagccttc
     6841 tgggcaccac ccccgccacc cccgtcgacg gacatcgacg atgaatcgtc cgcccatggt
     6901 aacgttgatt tgcttggctt ggacaagcgg acctgggcct atatcgcagg tggagttggt
     6961 gccgcgggtg tggtcacctt cggtgtcttc ggtgcgctaa accagtcgaa atacaacaac
     7021 ttacaggatg attgcccgaa cggcaggtgc ccttcggatc gtagcgacga catcaatgcg
     7081 ggaaggcgtt accaaacgat tgccaacgta ggtctcgtcg tgggggtggt cggattgggg
     7141 acaggaacag cgctttactt cctatccaag gattcggggc gtgaacagcc taccacgcag
     7201 gttgggtttg gacctggctc ggttactgtg caaggaacat tctgatgaac aagaaactcg
     7261 tatgcttggt tgtgcccctg tcgatgtggt ttttgggctg cacgaacgat tacgacgagt
     7321 tttcgttcca acccggctct accgatgggg gcgcggacag tagcaagggc ggcagcggtg
     7381 gagccacggg cggcagcggc ggagccacgg gcggcagcgg tggagccacg ggcggcagcg
     7441 gtggagccac gggcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcggc
     7561 agcggtggag ccacgggcgg cagcggtgga gccacgggcg gcagcggtgg cgagaccgga
     7621 ggcagtggtg gagccacggg cggcagcggc ggggccacgg gcggcagcgg cggagcagca
     7681 ggatgtggtg gaacccagaa gctttgtggc agtacctgcg tggacctcga tgacccgcaa
     7741 acaggttgcg cagaccccaa ctcctgtgat ccatgtgtca ttcccaacgc caccgctgcc
     7801 tgtgaattgg ggcaatgcgc catcggcaca tgcaataccg gcttcgagga ctgcgatcat
     7861 gatacggcaa cgggctgtga tgccgagctt gccgtcagca ccgaccattg cggcgcatgc
     7921 gcgcgtgctt gttccgatgc ccatgtcgtg acgccggtct gcgagaaggg cctttgcatg
     7981 tctacatgcg agacgggcta tggcaattgt gaacaaccgg caacgggtga tgatgatggt
     8041 tgcgaaacga atttaaaaga ctcgaccgac cattgcggtg gttgtaacaa tgcctgcccc
     8101 tccaacgcgg cgcagcattt cgtatgtcgt gacagccgat gcgcttgtga cagcaacgag
     8161 gattgcagca tcggtggggc aaccggaacg tgctatttga gtaatccgag tgactatcgt
     8221 tacggccgct gccggtgcgg aaacaacgat tgtcgtccgg gggaggtttg ccatactgtc
     8281 aatagtcagt cacgatgctc atgtaatggg ggccaaggct gctcgacgag ccagacttgt
     8341 tgccaaaccc ctgccggctg tttctacctc aactgggatc gcaacaactg cggcgcctgc
     8401 ggacacgtat gcccaacggg acaaaactgc atcgacggca gttgccattg actcgtcaga
     8461 cgcgttagcg tcgccctttg aatcccatca ttccctgcac caccgactca ctttgctcgg
     8521 caatctcatc ctgctcccgc gcgcgatccg cacaatcctc acataatttc agggtacgcc
     8581 ctcccacttt gacacggacc agctcgtcta cctcgtctcc acattcctta caaactggca
     8641 tatttcaacc tccaaaccgt gtttaattcg ggttttcacc acgcacaagc catcaccaac
     8701 gaattcctcc cccttgccca ctctcgaatc tcaccccctg gtggaaagta gcttccgtgc
     8761 ctcaacgacc aagttctgaa catcttgttg aagaagggga gccaactcgg ccgcacgccc
     8821 cttcgtcgtt cgcggtgttc ccatgcaaac cgccacatta gtccgttttc tcgcggcaga
     8881 acgctgtaaa tgcgcaacaa atgtctcgtc gacatactcc gtgcccggtt catacgccaa
     8941 accgacagga acgaactcca cgtcaaggcc gtttgctccc acgaaagccc cttttcggaa
     9001 aggacgaacc tcatcacccg caaaggtcgt gccttccgga aatacggtga tcgtagctcc
     9061 ttgttgaaga ctgcgacgga tctggcggat tgccgtggcc ccgctggcgc tctgatctcg
     9121 atcgacgaag atcgtttttg cttttcgcgc agccctgcca aggatcggcc actgggaaag
     9181 gtccgcccgg ctcaagatct ggcccccaaa tagggaaaga agcaccgcaa tatcgatggg
     9241 cgagcggtgg ttggcgacaa tcatccgtgc ggcggctccc tttcgtggcg caagggctgg
     9301 cgattgacgg acgtgaacgc caaaaagacg caacaacgtc cttacccata gccgcatgtg
     9361 acgatcgaaa ataccgtcag catgatcgcc agaagcgagg cgttggtgga gttcgacggc
     9421 gccgagctgg gcgaccgtga gcaaagcaag gccagaggcg cggtacgata cgcgtacatg
     9481 gctgacgaga cgacgtgcca tgggtggttg ctaccgctgt cgctcccccg acacaagagg
     9541 tctatcgaat ctccaacgag gacggcgccg ggggtgaaaa ggaacgcaac ttttcccttt
     9601 tgggcgaccc tcgtacaatc cccacccatg tctttccacc tctcgacagt catggtcttt
     9661 ttcctgctgc tcatgggatg ccaaccctcc agccctcccc aggcggctgc ccccccgttg
     9721 acaccccccc taacgacaac tcctccgcct gtcgccaccc ctcccacgat gccgacggcc
     9781 gactcgcagc cccccgtccc ggaaccagaa tccgccgagc ccacgatgcg ccccttggag
     9841 atcccttttg cgcagcttgt acaaggaata tctgaaaaag acgcatatta tttttcagat
     9901 aattacgttt cgaacgaaac cgcctttttg caggtagcgc ccgaactccc caaggcagtc
     9961 gctcccggag gcgccttcct cggtgtcggc cccgaacaaa acttcagtta tatcgctcta
    10021 acccggcccg atatcgcgtt tgtcatcgat attcgccgac gtaacatgct tcttcacttg
    10081 ttatacaagg cgattttcga agaagcttcg tcccgatctc attttttggc cttgttgctc
    10141 ggacgaaacc acgacacgtc aaccgatccc ggcgatggcg cgtcgatcga agacgttctc
    10201 gcgcatgcca ccaaagtcgc caaatcccgt gaagccttcc agacgatcca tcaacgtttg
    10261 gtggatcgca ttgcccacga ctatggcatt accctggact tcggcgatag gaacgcgctg
    10321 cgggaatccc accgggagtt tttcgacaaa cagcttgaaa ttgcctttga attgcacatg
    10381 aagtcggcgc gaaagtatcc aacgttgcgc acgctgctca ccatgcgcga cccctccggc
    10441 acccagacga gttttttggc ggatgaaaag gcatttcggc tcgtacagaa aatgcaaaag
    10501 gaaaaccgaa tcatccccgt ggttggcgac ttcggtggcg acaaagcctt gctcggaatc
    10561 gctgaattcc tccgcgccaa ccagatccgc ctgagcacgt tctatacctc gaacgtagag
    10621 gaatacctgc tgcaagacca taaatgggat aagtggatcc gcaatctcga cgctttcgct
    10681 atcgatgacc gcagcgtttt cattcgctcc tacctcgccc gggctggccg gacccatccc
    10741 ctgcagctcg aaggtcactg gtccacgact tttcttcaac gaattcaatt atttagagat
    10801 agagagaggg agcaccagtt ccgacaatgg cacgatgtcg ccacctacga attgatgacg
    10861 cctccatgac tttcccgaac cagcgaccat cgggccgaaa gccggtaacc atataagtta
    10921 attatatatt ttgtggggtt gccctacttc cacgcgccgc cgtcccatac catatctacc
    10981 ggttttccgg ttcgcggaac ttgcacctct ttggtgacca ataccggcgc ctgggtcggc
    11041 ccttgagata cgatggcgtc tacttgcacc tttatcggaa gcgacggtgg tagcgcctcc
    11101 tccgcggtta gctgtagaac gacatcgttg tccccgggat ccatgaccag tcggagcata
    11161 tcgatggcct ccgcggaaat atccggcttc cgctcttctt tcgcctcttt cggaccccgc
    11221 cgaaggatgg ccgaaagcgc gggagacgca ctcgcggaac gcaccaacac caccgtgccc
    11281 gaagtgcgtc tcggcaattc catggctcga cgcgtcagtt cgtcttgttg cggcttggag
    11341 attccctcgt gaggcgtgag aagcaacacg gtggccaagt ccgaagccag gtccgatagg
    11401 tccgaggtgg tgcttcgccc gccgttttgc gtgagacgcg tgagcattct tcccgctaga
    11461 tcgatgcgat tggctccagc atgagcaagc gccaaccgca tgagcacggg ttgatcgaag
    11521 ggcatggctc gttcgagcgg tgcgtacatc gaaaccgccg catcgtacca tccttcccgt
    11581 cgaagcctat cgcccgtgta cgcccttgcc cacggatcgc gcggggcgcg ctcgacgatt
    11641 tcagaaaacg tgcgaagagc ttccgcttca tggcccaaag aacgcagcgc cgtggccgca
    11701 tccgccaaaa gcgttgcatc gctgaacgga tccctgcgaa tccttgcgat ctcctgccgc
    11761 aaagcgtctt ttccccctgt cgattccagc aacgtcaaca tcagtcgctg aagacgaaca
    11821 tcgtgggggg ctagtgtgag gtatcgtcgc acaatctcca agcgagaaag atccgatgtt
    11881 gctgcactat actgcttttc gaaaatatcg acgggatacc cttcatcgag caacagggct
    11941 gctcgcacac gccgcatttc atccggtgaa ttcgcccggc gaagcgc
//
LOCUS       DAOSOR010000107        11969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5295_length_11969_cov_4.040037, whole genome shotgun sequence.
ACCESSION   DAOSOR010000107 DAOSOR010000000
VERSION     DAOSOR010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11969
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5295_length_11969_cov_4.040037"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="PK710_07965"
     CDS             <1..1053
                     /locus_tag="PK710_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="expansin-like protein"
                     /protein_id="HOT09690.1"
                     /translation="PSFVREGMTLAIAEPYNRSSYEGEVGESCGECWELDTLADTRIV
                     MVHDLCPIEGNPLCAGGHFHFDLSGEAAKVLDAQGLFEGQTRRVPCPVDGNVHIQILD
                     RNEWGYVRFQFVNHRIPIRNADYRADNDTVWRPVQRSGGAWHVLDDNKTFASGSPGGV
                     FRITSAQGEILECPRVLPYAQSKGTVFDLGAQLTDQHPSSGGTCVFEPPRVVFDDDYG
                     GIDKVRWTMNPWGGASDSKVTEGCFQGACIRVDNLGQWEGFHIYYRQAFPPSIFSKLT
                     MRVRSTLENSEITVAPSYQGDRCQETSVNVETDWSDVSIEIASVCAEQPLIDAVTVSN
                     QGKPTVILLDDVMFEK"
     gene            complement(1233..1619)
                     /locus_tag="PK710_07970"
     CDS             complement(1233..1619)
                     /locus_tag="PK710_07970"
                     /inference="COORDINATES: protein motif:HMM:NF021193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HOT09691.1"
                     /translation="MPEFLSQLAGNPWLWFIGTSVSIVLFVATLVGIPILCIRLPADY
                     FIRPHQPQPIWKVTVRFVVASIVILMGIAMLVLPGQGLLTLLIGISLLDFPAKRVWQR
                     RLLQHPPVYRALNAIRKRAGKTPFQT"
     gene            complement(1680..2546)
                     /locus_tag="PK710_07975"
     CDS             complement(1680..2546)
                     /locus_tag="PK710_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09692.1"
                     /translation="MTSSDHSGHEASRSDDHATSQGLEQPDVRWWGWLGLLIPPCVLS
                     FAARFAPQVNVGEFWGDQLTYLGSVFTISTTLYVLSYTKSTRLWFGFILFALLSLLIV
                     SLTDSPGVAAFVMIGTALMTIGHGIGGMIGSRIQHPGHLLPACVVAASFDIASVIHPQ
                     GPSHALVSSEHALPWLTLSFPVFGARAFSPNVGIGDVVFAALLLGAAARHGLSRPRFV
                     ALITAGLIIAGQLAALLQQAIPALPIIGICIVVGVAPARIMRRKERRVAFWFMAASVT
                     LALGVIASRFLA"
     gene            2952..3380
                     /locus_tag="PK710_07980"
     CDS             2952..3380
                     /locus_tag="PK710_07980"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOT09693.1"
                     /translation="MTSFLLAAAMVLAFSGCGKKEELPAGPGVTVTEKKDEFKIEDVK
                     VGDGAEAKENSAVKVHYTGTLKNGKKFDSSRDRDAPFEFTIGLGQVIKGWEKGVVGMK
                     VGGQRKLTIPYDLAYGEEGKPPDIPPRATLLFDIELLEVK"
     gene            complement(3411..5003)
                     /locus_tag="PK710_07985"
     CDS             complement(3411..5003)
                     /locus_tag="PK710_07985"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT09694.1"
                     /translation="MRREEMIARVDDHRRPWDMLFVGGGATGVAGALDAASRGYEVLL
                     LEQSDFGKATSSRSTKLVHGGVRYLQQGNLPLVMGALHERGIMRQNAPHLVRDLGFVV
                     PNYTWWEAPFYGIGMKVYDMLAGRYGFGRSRILSFQETVDRIPTISLEGLRGGVAYYD
                     GQFDDARMLLNMAQTAAEQGATLLNYTRVVGLTKNSEGFVDGVIALDQETGKERTIHA
                     KVVVNCTGPFADELRRMNDPEAQRMIAGSTGVHIVLDRSFLPGDNAIMVPRTADGRVL
                     FAIPWYDKVVIGTTDVPTSDFVLEPWPTDEEVDFILENAEGYLDRSPGRGDILSVFSG
                     IRPLVKAADDANTSQLSRDHTITVTKTGLVTIAGGKWTTCRHMAEDLIDNAMVVADLP
                     PQPCVTGQLRIHGYHRYADKFGEFAFYGSDAPAIQDSLRRHPEYAERLHPRLPLVAGQ
                     VVWAVREEMARTVDDVLARRTRSLLFDAQAAIEASPKVAKWMAAELGKDELWVADQIR
                     DFLQVAKRYLVSELVSHHREKA"
     gene            5337..5966
                     /locus_tag="PK710_07990"
     CDS             5337..5966
                     /locus_tag="PK710_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09695.1"
                     /translation="MFTDGGDVLSHGDSADGHSGSGGGSAVGGAGGVGGAGGTGGIAN
                     TGGVGGSESGGAGGSAQAGTAGNGASAGSSGASGSGAAAGEAGSSGQTGGTGASAGFG
                     PGTLDPQLALPDASGAACEVGAFCWNNIYYACRIASPTDGRCETCLYAALCGVQGSSC
                     TKSNECGTLYQCYAGKCSSICKLGVDDPLCDAQDCRDVGHVTHGVCVRN"
     gene            complement(5978..6946)
                     /locus_tag="PK710_07995"
     CDS             complement(5978..6946)
                     /locus_tag="PK710_07995"
                     /inference="COORDINATES: protein motif:HMM:NF025059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOT09696.1"
                     /translation="MRERWAQDIDARARSYWTEARTRGLLGTKHLILPPSEYGPLLRA
                     MGLLKADASMGPESVRKYMQISHMLTLIEPQIEELKRQNSEVFILDLACGNSYLTFTI
                     ATSLAHRLRHPGQVLGVDRNLRLIEACRERAWRLGLEDILRFEASNIEEVDVQKAWQR
                     AFGKNADGTVHLLLALHACDTATDEALALGISWNVPSMAVAPCCQGELAKTWAEGEFD
                     DAFMPIRRWPHLRREVAATMTDALRALILEGCGYEANPIEFVPSAHTPKNTLLRANRR
                     GQGSPESFREYVALRNALGGMSIHLERLLTEPHRSHLNALLMQRPH"
     gene            complement(6996..7196)
                     /locus_tag="PK710_08000"
     CDS             complement(6996..7196)
                     /locus_tag="PK710_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09697.1"
                     /translation="MTSPWKRILFTSMAAAGGAGVGWFYAVVINSCGGTCLVGAQPWI
                     VIVGLALVGGYAAMVATRPQAQ"
     gene            complement(7232..7717)
                     /locus_tag="PK710_08005"
     CDS             complement(7232..7717)
                     /locus_tag="PK710_08005"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOT09698.1"
                     /translation="MTAIPFSTLNVLRRCHDLEVLMEKVYRELAQQHADMPSLSALWL
                     KTAAEERNHASQFSLAMSLGGDVVPSSTVDIQQVEDAFSSASALLRRVRSSRLLPREA
                     LEIAISLEERFAIFHADAAIAFSSSSHEKLFHAMMAADKQHVDALRQELQRLSPAKQP
                     D"
     gene            complement(7828..8493)
                     /locus_tag="PK710_08010"
     CDS             complement(7828..8493)
                     /locus_tag="PK710_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT09699.1"
                     /translation="MKQCSGLVVETITNGPFVENCYLVADPITRKAILIDPGDEEDRI
                     ADKVKQANVQVVEIVNTHGHIDHAGAVASLKRLLDVPFAIHPGDRGWLEHLGSSAVAF
                     GLSPRPTPEVDRDLEHGQTLMVGEVKGIVLHTPGHTEGGCCFYFPEPRAVFVGDTLFA
                     GSIGRTDLPGGSSSTLLRSIQEHLLTLDDDVVVYSGHGPTTDIGTERRSNPFLRPGGP
                     IFF"
     gene            complement(8555..9844)
                     /locus_tag="PK710_08015"
     CDS             complement(8555..9844)
                     /locus_tag="PK710_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOT09700.1"
                     /translation="MKPKVFCIIMGGGRGRRLRPLTKLRAKPAVPLAGKYRLVDIPIS
                     NCLHSGLNHISILTQFNSASLHQHIQDTYRFDSFSGGYVDILAAEQTIEGTSWYQGTA
                     DAVRHNLRHFSAHDGDLFLILSGDQLYRMDYQELLRQHHRTGARVTIAAKPLNCNRVD
                     QFGVLRVADDLSVTAFVEKPTDPEVVRQMQSWLPPRPNGTATPTGVCWVNMGIYVFEK
                     RTLWDALTQNSLSDFSKEIIPSLVGTVPIYAFPFEGYWRDIGTVRAFFEANLELTSPS
                     PPFNFFEPGRVIYTHPRFLPASRLQRCTIDQAIVADGCIIIDAVLERSVIGVRSMIHE
                     GSRLKNVVMMGQDQYESPEAETSAKARGLPPIGIGKSCFIENAILDKNARIGDDVSLS
                     PAGLPDDFEQGDVAVRDGVLIVTKNGVVPSGSRIGNA"
     gene            9939..10109
                     /locus_tag="PK710_08020"
     CDS             9939..10109
                     /locus_tag="PK710_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09701.1"
                     /translation="MSPLPYAPAQLVCVRNHEFLRESIPFDESRFAWLIDRAWMRPGS
                     ETPRKAASAVMS"
     gene            complement(10117..10458)
                     /locus_tag="PK710_08025"
     CDS             complement(10117..10458)
                     /locus_tag="PK710_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09702.1"
                     /translation="MASLLVVGCSTTSGAHCRPVENGCFLRLYDGDDFEKSEACLVID
                     EPGRYANLDQVPGATQNWTGEADSFEVGPQATVTVWSERNFSGNSVIYAPGARIRSVE
                     PEPASLELRCE"
     gene            complement(10758..>11969)
                     /locus_tag="PK710_08030"
     CDS             complement(10758..>11969)
                     /locus_tag="PK710_08030"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOT09703.1"
                     /translation="ETGGTGGGETGGTGGGETGGTGGATGGTGGGTGGSGGGVVPCNG
                     TIEQLRTEATGANKKPVDLEVCDVVVTYVLSTGYFIQKDPTGPAIQVFEGNSWTPNVA
                     VGDVIDMRVTQVTHFNNQEEVSGHEPIVKKSSGTDITPLIQNLTVAPTEDYESELVKI
                     TGGTIVTLNGKDGTINYGTATNVVLRAESTGTLCVGAKFDLLAPVSQYQADHRLQSWA
                     LKDFSNIDTSGCATGTGKIPNVGDLIINEVMGNPPLDLEGDTNCDGTRDASKDDFVEI
                     VNNTDEVLALGGITIADSEKVRFTFPTPTDLPARGVVVVYGGGEAKCTYPANVMTFIS
                     SGLALGPDTDSVKITRGDGTELASMTYSGTQNDRASWTRNPDLTGNFVLHNTLGALKF
                     SPGTKNDGTPF"
BASE COUNT         2681 a         3427 c         3328 g         2533 t
ORIGIN      
        1 ccttcgttcg tgcgagaggg gatgacctta gcgattgcag agccctacaa ccggagttcc
       61 tacgagggag aagttggtga atcatgcggg gaatgttggg aactcgatac gcttgcggat
      121 actcgcatcg tcatggttca cgatttgtgt ccgatcgaag ggaacccttt atgcgcgggc
      181 ggtcactttc atttcgatct ttccggggag gccgcgaaag ttctggatgc ccaaggtttg
      241 ttcgaaggac aaactcgccg cgttccgtgt cccgtcgacg gtaacgttca tattcaaatc
      301 ctcgaccgaa atgaatgggg atatgttcga ttccaattcg tcaatcatcg gatccccata
      361 cgaaacgccg actacagagc ggataacgat acggtctggc gccctgtgca acgaagcggt
      421 ggagcatggc acgtcttgga tgacaacaag acctttgctt cgggttcccc tggcggcgtc
      481 tttcggatta ccagcgcaca aggagaaatt ctcgagtgtc cacgggtgct cccctacgcc
      541 caatcgaaag gaacggtctt cgatctggga gcgcaactca ccgatcagca tccatcttct
      601 ggaggcacct gcgttttcga gccaccgcgt gtcgtattcg atgacgacta tggcggcatc
      661 gacaaggttc gttggaccat gaatccatgg ggaggagcat cggattccaa agtaaccgaa
      721 ggttgtttcc agggagcatg cattcgtgtc gacaacctgg ggcagtggga agggtttcac
      781 atttattatc gacaagcatt tccgccatcg atcttttcca agctgaccat gcgcgtgcga
      841 tcgaccttgg agaacagcga aatcaccgta gccccaagct atcaaggcga tcgctgtcaa
      901 gagacgagcg tcaacgtgga aaccgactgg tccgatgtat cgatcgaaat cgcatcggta
      961 tgtgccgagc agcctctcat cgatgcggtt accgtgagca accaaggaaa gcccaccgtc
     1021 atcttgctgg atgacgtcat gttcgaaaaa tgattggctt cggccagcag tgttagcgac
     1081 tgcaactccg cacggcggag gaacgcgatc actatgctgc gtcatcagtc gatacaggtc
     1141 cgtggctgta tcgatggctc atgcgaaacc gtgtccctta ggcagcgctc cacgccaatt
     1201 ctcatcgttt catggtctca tgaccgaaac cctcatgttt gaaatggcgt tttgcccgcg
     1261 cgctttcgta tcgcattcaa agctcgatac accggtggat gttggagtaa ccgacgctgc
     1321 cacacgcgtt tggctggaaa atcaagaagc gagattccta tcagtagtgt caggagaccc
     1381 tgtccaggaa gcacgagcat cgcaatgccc atgaggatga caatgctggc cacgacgaac
     1441 cggacggtca ctttccatat cggttgaggc tgatggggac gaatgaaata atccgcgggt
     1501 aagcgaatgc acaggatggg tatgcctacg agtgtcgcta cgaacaagac gatggatacg
     1561 ctggtgccga tgaaccataa ccacggattg cccgcaagct gactcaagaa ttcaggcacg
     1621 agtcattcat aggacagagg cttgatggat gcacgcggat gtggcccgat gcccgatcgt
     1681 catgcgagga atcgagaggc aatcacgccc agcgcaagcg taaccgatgc tgccatgaac
     1741 cagaaagcaa cacgtcgttc tttgcgtctc atgatacgcg ccggtgctac cccgaccacg
     1801 atgcagattc cgatgatggg aagcgcggga atggcctgtt gcaagagcgc ggctagctgt
     1861 ccggcgatga tcagtcccgc ggtgatcagt gccacaaacc gtggtctgga caatccatgc
     1921 cgagccgccg caccaagcaa taatgcagcg aatacgacgt ctccaatacc aacgtttggt
     1981 gagaatgctc tcgctccgaa cacgggaaac gataacgtca gccacggtaa tgcatgttca
     2041 ctgctcacga gcgcgtggga ggggccttgg ggatgaatga ccgaagcgat atcgaacgat
     2101 gcggccacga cacaggcggg taggagatgc cctggatgct ggatacgaga gccgatcatg
     2161 ccgccgatcc catgaccgat cgtcatcaac gcagttccta tcatcacgaa agcggcaacg
     2221 ccggggctgt cagtgagtga aacgatgagc agcgatagga gggcaaaaag gatgaaaccg
     2281 aaccataatc gtgtgctttt cgtataagac agaacgtaaa gggtggtgga aatcgtgaat
     2341 acggagccga gatacgtgag ttggtctccc cagaattccc ccacattgac ctggggggcg
     2401 aaccgagccg cgaaagacaa aacacatgga gggatgagaa gccccagcca cccccaccac
     2461 ctaacatcgg gttgctcgag cccctggctc gtggcatggt catcgctccg ggaggcttca
     2521 tgcccagaat ggtctgacga agtcatttcg ttcccgactc ataacgtgga cccaggtttt
     2581 ctgtggtccc cgccatggaa cacccaatgc ccagcttgca acgacggctg gtgggtgccg
     2641 gcacgccgga ggaggtacgg acaacatgaa cagagcccgt aaacccaacc cggaggtggt
     2701 cacgtaggcc gtcaggccat ggcaccgtgc cccgaatggg ttggggcagg gaccgtgggc
     2761 cgggagcgac aagctgttcc cggcccgcgt tgcttttgtc gctcgtggga tgacaagtcc
     2821 gtagctctgc ggtagggcag cttctgcgtg agatcctcat gcttccaacc gctccccgca
     2881 cgtttgcgcc tgctgggggc ttgaaccacc aactcctacg aaaccagatg gataggcgaa
     2941 cgatgcgatg catgactagc ttcttgttgg ccgccgcaat ggtgctggct ttttcgggtt
     3001 gcgggaaaaa agaagaactt cccgccggac ccggcgttac ggtcacagag aagaaggatg
     3061 aattcaagat tgaggacgtg aaggtcggcg atggcgcgga agccaaagaa aactccgccg
     3121 tcaaggtgca ttacaccgga accctaaaaa acggtaagaa attcgatagt tcccgcgatc
     3181 gcgacgcacc gttcgagttc accatcggcc tgggacaggt catcaaaggc tgggaaaaag
     3241 gcgtggtcgg catgaaagtc ggcggtcaac gaaaactcac catcccctat gatctcgcct
     3301 acggcgaaga aggaaaacca ccggatatcc ctcctcgggc caccctgctt ttcgatattg
     3361 aattgctcga ggtcaagtga accttcacgc agcaatcctt cggctgtttc tcatgccttc
     3421 tccctgtgat ggctgacgag ttccgatacc aggtatctct tcgccacctg caaaaagtct
     3481 cggatctgat cggcaaccca taactcatcc ttgccaagct ccgcggccat ccacttcgcc
     3541 actttgggag atgcttcgat ggcggcttgc gcatcgaaaa gcaacgaacg cgttcgacgc
     3601 gccagaacgt cgtcgaccgt tctcgccatc tcttcgcgta ccgcccatac gacttgcccg
     3661 gcgacgaggg gaagacgagg atgaagccgt tcagcatatt cgggatggcg acgaagagag
     3721 tcttggatcg ccggcgcgtc ggaaccgtaa aaagcaaact ccccaaattt gtccgcatac
     3781 cgatggtatc catggattcg aagctgcccc gtgacacacg gctgcggcgg cagatcggcc
     3841 accaccatcg cattgtcgat caaatcctcc gccatgtgcc gacaggtcgt ccacttgcct
     3901 cccgcaatgg tcaccaatcc tgtcttcgta accgtaatcg tatgatcccg actcagttgc
     3961 gaagtattgg catcatccgc ggctttgacg agcggccgta tccctgaaaa tacgctcaaa
     4021 atatcgccgc gcccaggaga acgatcgaga tatccctccg cattctcgag aatgaaatcc
     4081 acttcctcgt ccgtgggcca aggctccaac acgaaatccg atgtcggaac atccgtcgtc
     4141 ccaatcacca ccttgtcgta ccaaggaatg gcaaatagaa cgcggccgtc cgccgttcgc
     4201 ggcaccatga tcgcgttgtc gcccggcaaa aatgaacgat cgagaacgat atgaaccccg
     4261 gtgctacccg cgatcatccg ctgcgcttcc ggatcgttca tgcgacgtaa ctcatccgcg
     4321 aaaggtcctg tgcagttgac gacgaccttg gcgtgaatcg ttcgttcttt gcctgtctcc
     4381 tgatccaagg cgatcacacc gtccacgaac ccttcactgt ttttcgtaag ccccaccacg
     4441 cgcgtgtaat tcaacaacgt cgccccttgt tccgctgccg tctgagccat gttcaacaac
     4501 attcgggcgt catcaaactg gccgtcataa taggcaacac ccccacgcag tccttcgagg
     4561 gagatggtgg gaatccgatc gacggtttct tgaaagctga ggatacgact tcgaccaaac
     4621 ccatatcggc ccgcgagcat gtcgtaaacc ttcatgccga tgccgtaaaa aggcgcctcc
     4681 caccaggtgt agttcgggac gacgaatccg aggtctcgca cgagatgagg agcgttttgt
     4741 cgcatgattc cgcgctcatg caacgctccc atcaccaacg gtagattacc ttgttgtaga
     4801 tagcgcacgc cgccgtgaac caacttcgtg ctccggctgc tcgtcgcttt gccaaaatcc
     4861 gattgctcga gaagcaatac ctcgtaaccc cgtgatgccg catcgagcgc tcccgcgact
     4921 cccgtcgctc caccccccac gaaaagcata tcccacgggc gtcgatggtc atctaccctc
     4981 gctatcatct cctcgcgtcg catgatggct cctctctctg aaggggatgc tagcaaacca
     5041 atcctaggaa cctcgcgcaa ggcgattgat gccatgatgt ccttggcctt cttgctttgg
     5101 gacggtctgt tcgctcatcc cgtcgcgaag ccttcagccc caatccggaa tccttttcga
     5161 tccgttcctt tggaattttc gctatttttg cgggaaacga agggcttgct cgtacgttcg
     5221 gctcgcgtac actgcggagt atgtgcaaat cgctatggcc aaagtacgtc gttttcctct
     5281 gcctaggtgg ctttggattg ggagcatgcg caacgggtga ggatggggaa cctcgcatgt
     5341 tcaccgatgg cggagacgta ctatcccacg gtgattcagc cgacggtcat tccggttctg
     5401 ggggtgggtc cgcggtggga ggagcgggag gtgtgggagg agcgggaggt acgggaggca
     5461 tcgccaatac gggcggcgta ggtgggtccg agagcggcgg tgctgggggt agcgcgcaag
     5521 caggaacagc aggaaacggc gcttcggctg gtagcagcgg cgcctccggc tccggggcag
     5581 cggctggtga agctggttcc agtggtcaaa ccgggggcac cggcgcttcc gccggattcg
     5641 gtccaggcac tctcgatccc caacttgctc ttcccgatgc ctccggggca gcctgcgaag
     5701 tcggcgcttt ctgctggaac aacatttatt acgcctgccg tatcgcttcg cctaccgatg
     5761 gccgatgcga aacatgcttg tatgccgcgc tttgcggcgt ccaaggctct tcttgcacca
     5821 aaagcaatga atgcggaacc ctgtaccaat gctacgccgg caagtgctcg tcgatttgca
     5881 aattaggcgt cgacgaccct ctctgtgacg cgcaagattg ccgcgatgtc ggacatgtca
     5941 ctcatggtgt atgtgttcga aattaaaagg tattttacta gtgcggcctt tgcatcaaaa
     6001 gcgcgttcag atgggatcga tgcggctccg taagaagcct ttcgagatga atcgacattc
     6061 ctcccaaagc gtttcgcagc gccacatact cacgaaatga ctctgggctc ccttgtcctc
     6121 gacgattcgc tcgcaatagc gtgttctttg gcgtatgcgc cgacgggacg aattcgatgg
     6181 ggttcgcttc gtaaccacat ccctcgagaa tcagtgctcg caatgcgtcc gtcatggtcg
     6241 ccgctacttc ccgccgcaga tggggccagc ggcgaatcgg catgaaagca tcgtcaaatt
     6301 cgccttccgc ccacgtctta gccaattctc cttgacagca tggcgctacc gccatcgatg
     6361 gcacgttcca cgagatacca agcgccaacg cttcgtccgt cgccgtatcg caagcatgaa
     6421 gcgccagcaa cagatgcacc gtgccgtcgg cgttttttcc aaatgcccgt tgccaggctt
     6481 tctgcacgtc gacctcttcg atattcgacg cttcgaaacg caggatatct tccaatccca
     6541 atcgccacgc gcgctccctg caggcttcga tgagccgaag gttgcgatcc actccgagca
     6601 cttgccccgg gtgccgcaag cgatgagcca gactcgtggc gatggtgaaa gtgaggtaag
     6661 agttgccgca cgcaagatcc aagatgaaaa cctcggagtt ttgccgtttg agttcttcga
     6721 tctgcggttc gatcagcgtg agcatatgac tgatctgcat gtacttgcgc acggactcgg
     6781 gccccatcga agcgtcggct ttgagcaaac ccatggcgcg aagcaacggt ccatactccg
     6841 aagggggaag aatcagatgt ttcgttccca atagcccacg ggttcgcgcc tccgtccaat
     6901 aggaacgggc gcgcgcgtcg atgtcctgtg cccagcgttc tcgcatggca aagcgccctg
     6961 taatcgcccg tatcggccgt gacaagagcg gcgtgtcatt gcgcttgtgg tcgcgtcgca
     7021 accatggccg cgtaacctcc cacgagcgcc agacccacga tgacaatcca cggttgagcg
     7081 cccacgagac aagtaccccc gcaggaattg atgacgacgg cataaaacca accgacgccc
     7141 gcgcctccgg cggcagccat cgatgtgaaa agaattcgtt tccacggcga agtcacggca
     7201 aatcgttaga gacgactgct cgccattgga ttcaatctgg ttgtttcgcg ggcgatagtc
     7261 gttgcaactc ctgccgcaac gcgtcgacgt gttgcttgtc cgcagccatc atcgcgtgaa
     7321 agagtttctc atgcgaggag gaggaaaagg caatggctgc atccgcgtga aaaatggcga
     7381 accgctcttc cagagaaatg gctatttcca aggcttctct ggggagcagc ctggaagagc
     7441 gaacacgacg caacaacgcg gatgccgaag aaaaagcgtc ttcgacttgc tggatatcca
     7501 ccgtggaaga agggaccaca tcgcccccta acgacatcgc aagagaaaat tgggaagcgt
     7561 gatttcgctc ttccgcggca gtcttcaacc acaaagcgga aagactcggc atgtccgcat
     7621 gttgctgcgc caactcccgg tatacctttt ccatcaacac ttcgagatca tgacatcgtc
     7681 gcaagacgtt cagtgtggaa aagggaattg cggtcattct gataggctat ccaatcagcg
     7741 cccgagaaca agccagggaa tgagtcaagc caccgcggag gctggccaaa acctttggat
     7801 gccgtcgttt ctgtggtgaa cgatcggtta aaagaaaatg ggtccgccag gacgaaggaa
     7861 agggttcgac cgacgttccg ttccgatatc cgtggtagga ccatgaccgg aatacaccac
     7921 cacgtcgtca tccaacgtca gaaggtgctc ctggatcgaa cggagcaacg tggatgatga
     7981 ccctccaggc aagtccgttc ggccaatgga gccggcgaat agcgtatcgc cgacgaaaac
     8041 ggcacgcggc tccgggaagt agaaacaaca tccgccttcc gtatggccgg gtgtgtgaag
     8101 cacgattcct ttcacctcgc cgaccatcaa cgtttggcca tgctcgagat cacgatcgac
     8161 ttcaggcgtt ggcctgggtg aaagaccgaa agcaaccgcg ctcgacccca aatgttcgag
     8221 ccagccccgg tcgcccggat gaatcgcaaa cggtacgtcg aggagccgct tgagcgaggc
     8281 cacggccccc gcatgatcga tgtgtccgtg cgtgttgaca atctcgacca cctgcacgtt
     8341 cgcttgcttc accttatccg caatccgatc ttcctcgtcg cccgggtcaa tcaagatcgc
     8401 tttgcgggtg atgggatccg caacgagata acaattttct acaaaaggac cgttggtgat
     8461 cgtctctaca acaagaccgc tacattgctt catgggttgt ccagagtcag ccatcatgca
     8521 tgggttgaag ggatttgaac cgaaccgctt ttcatcatgc gttgccgatc cgcgatccgg
     8581 aagggaccac gccatttttc gtcacgatga gcacaccatc gcgtaccgct acatctcctt
     8641 gctcgaagtc gtcgggcaag cccgcgggag acaaagaaac atcgtctcca attcgcgcgt
     8701 ttttatccag gatggcgttt tcgatgaaac aggacttgcc gatgccaatg ggcggaagcc
     8761 cgcgcgcttt ggcgctcgtc tccgcttcgg gggactcgta ttgatcctga cccatcatca
     8821 cgacgttttt caatcgcgac ccttcgtgaa tcatcgaccg cacgccgatc acactacgtt
     8881 cgagcacggc gtcgatgatg atgcagccat cggccacgat cgcctggtcg atggtgcatc
     8941 gttgcaaacg agaagcggga aggaagcgag gatgggtgta gatgacacgt ccaggttcga
     9001 aaaagttgaa cggtggggaa ggggaggtca actcgagatt ggcttcgaaa aacgcgcgca
     9061 cggttccaat gtcgcgccaa tatccctcga agggaaaggc gtagatgggc accgttccca
     9121 ccagactggg aatgatctct ttgctgaagt cgctcaaact attttgtgtc aatgcatccc
     9181 agagggtccg tttttcgaat acgtagatcc ccatgttgac ccagcacaca cccgtgggtg
     9241 tagccgtgcc gttgggacgc ggtggcaacc atgattgcat ctgtcgaacg acttccgggt
     9301 cggtcggctt ttcgacaaac gccgtcacgg ataaatcatc cgccacacga agcacgccaa
     9361 attgatccac gcgattgcaa ttcaaaggct tggccgcgat ggtgacacgg gcacccgtgc
     9421 gatgatgctg tcgaagaagc tcctggtagt ccatgcgata gagctgatcc cccgacaaaa
     9481 taagaaatag atccccatcg tgcgcggaga aatgccggag gttgtgacgg accgcgtcgg
     9541 ccgtgccctg gtaccacgac gtgccctcga tcgtttgctc agccgcaaga atgtccacgt
     9601 aacccccgct gaacgagtcg aatcgatacg tgtcctgaat gtgctgatgc aaggaagccg
     9661 aattgaattg cgtcagtatg ctgatgtggt tcaaaccact gtgaagacag ttgctgatgg
     9721 gaatgtccac gagacggtat ttgccagcga gagggacggc aggcttggct cgcaacttgg
     9781 tcaaagggcg caaccgacga ccacgtcctc cgcccatgat gatacaaaat acctttggtt
     9841 tcatggatcg aacctcatca tgccacgcca ccatggcata gatgaagcct gcctgtgatg
     9901 ataaagatgg tgaatttttt atcgagagcg tcccgtggat gagccctctt ccttacgctc
     9961 ctgcccaact cgtttgcgtg cgaaatcatg agtttttgcg cgaatcgatt cctttcgatg
    10021 agtcacgttt tgcatggctc atcgaccgtg cgtggatgag accgggctcc gaaacgccac
    10081 gaaaggcagc gagtgcggtg atgtcatgaa cgctcgctat tcgcagcgaa gctcgaggga
    10141 ggcaggttcc ggttcgaccg accgtattcg agcccctgga gcataaataa ctgaatttcc
    10201 agagaaattt ctttcgctcc acaccgtgac cgtggcctgg gggcccactt cgaagctatc
    10261 cgcttcgccg gtccagtttt gcgtggcccc gggaacttga tccaggttcg cataacgtcc
    10321 cggttcatcg atcacgagac aagcctcgga cttctcgaaa tcgtctccgt catagaggcg
    10381 aaggaagcaa ccattttcca cgggtctgca atgcgcgcca gaggtcgtgc tacagccaac
    10441 gaccaatagg cttgccatag tcaaacccat ccacagtgcc caggcggatg tctgccatgc
    10501 gtgcatcatc ggagcctccg tcatcgtcga ttcatcgttg aatcatgacg ctttttcatc
    10561 cgtggcacca catcgccgta acgcttcaca catggaggtg aacatggatt gcgaacatgc
    10621 tcgggcgaga tgtgcgcgat gcgttcgaaa atcaaacgaa gatacgcctc tttcgccaag
    10681 acggcgttgt ccgatgatgt aatggcaggt gaatcgcgcg tcgggatcga cgcgacaacc
    10741 catgcgagaa tcgcggatca gaacggggta ccgtcgttct tcgtgccagg cgaaaacttc
    10801 agcgcgccga gcgtgttatg aagcacgaag ttgcccgtca agtccgggtt tcgtgtccac
    10861 gatgcacggt cgttttgcgt gccagagtag gtcatcgacg ccagttcggt cccatctcct
    10921 cgagtaattt tgactgaatc tgtatccggg ccgagagcga gcccactgga gatgaacgtc
    10981 atgacgttcg cgggatacgt acacttcgcc tcgccgccgc cgtacactac aaccaccccg
    11041 cgagcaggaa gatccgtggg cgtggggaag gtgaatcgta ccttttcgga atcggcaatc
    11101 gtgatccctc ccagggcaag aacttcatcc gtgttgttga cgatttccac gaagtcgtct
    11161 ttggaagcat cgcgcgttcc atcgcagttc gtatcgccct cgagatcgag cggcggattg
    11221 cccatgacct cattgatgat caagtcgccc acgttcggga tcttgcccgt cccagttgca
    11281 cacccggagg tatcgatatt cgaaaagtct ttaagcgccc aggattggag ccgatggtcg
    11341 gcttggtatt gcgaaaccgg cgcgagaagg tcgaacttcg ctcccacgca cagcgttccc
    11401 gtactctctg cacgcaacac cacattcgtc gcggttccat agttgatcgt gccgtctttc
    11461 ccattgaggg tcacaatggt tccgcccgta atcttgacca actccgactc ataatcttcc
    11521 gtgggagcga cggttagatt ctggatgagg ggggtgatat ccgttccgct ggactttttg
    11581 acgatgggtt cgtggccgga gacttcttct tgattattga agtgggtgac ctgagtgacg
    11641 cgcatatcga tgacgtcacc cacggcaacg ttgggggtcc aactatttcc ttcgaaaacc
    11701 tggatggcgg gaccggtagg gtctttttgg atgaagtagc cagtcgatag aacgtacgtc
    11761 acgaccacat cgcagacttc gagatcgaca ggttttttgt tggcgcccgt tgcttcggtg
    11821 cgaagttgct cgatggtgcc attgcagggc acgacgcctc cgcccgatcc acccgtgccg
    11881 cctccggtgc caccggtagc gccgccggtt ccgcctgttt ctccaccacc ggtgccgccg
    11941 gtctcaccgc caccggtgcc gcctgtttc
//
LOCUS       DAOSOR010000108        11886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5340_length_11886_cov_4.450849, whole genome shotgun sequence.
ACCESSION   DAOSOR010000108 DAOSOR010000000
VERSION     DAOSOR010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11886
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5340_length_11886_cov_4.450849"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PK710_08035"
     CDS             complement(<1..535)
                     /locus_tag="PK710_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09704.1"
                     /translation="MRILAVYGVALLMFWAGPAFADELTVFGAGSRSAAMAGAGAALL
                     DDSYAAHANPAGMTLGQPGVSVGFVGAANRLQVRLADRPAGYDLPDLGASSPAIPSRY
                     RLTRRESESRAPGFSGFTVGATISGGIDWLRVGVLAFIPTTGIGNQNSYYADEREQYF
                     SNTVHQAIYGERLSTQQT"
     gene            complement(814..1680)
                     /locus_tag="PK710_08040"
     CDS             complement(814..1680)
                     /locus_tag="PK710_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT09705.1"
                     /translation="MDARWTLFLAATIAMACSSSPPPRPIAPAPSSAGSSSSPKPVSP
                     EVVPVSVHYRMVQAGPVEWLVDEVLLDASASRCFQARRRQIGGITFESRSATLDHSAF
                     STEGTRIVALWKGAKQGTKPAVVLHGFADHDEAQSAQAALELSRQRAEAVKHWLVQQG
                     ISADSLRVEAHGWDLSPAPLMPSDALLPNHRVEISHDTTPPSEAKILESKAIPEQWRQ
                     TLGQRVRALQGKEAAPGDSKLAPRIELSVRDGDGQTIVSFDLASSDEQGRALHRYFLS
                     GLAHGCEFGPYL"
     gene            complement(1858..3297)
                     /locus_tag="PK710_08045"
     CDS             complement(1858..3297)
                     /locus_tag="PK710_08045"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOT09706.1"
                     /translation="MAQSTWSLSYDCTVTEETGYTNGQSFPIQVVTVQGFPVEVKTAD
                     ALLTMMEAAENAGVPLKINDAFRTWDEQAYFYHCYTTCSCNNCNPAAPPGYSNHQSGH
                     AIDFDMGGGRGAWIFANAPSFGWDNDEGASVGEDWHWTWWGGGSPKAFCSNQPPWGSL
                     DHADDQSISGWAFDEDAGETAIDVHIYFNAPAGSDGAHGVSVLANVERDDLCSVVGSC
                     NHGFRMHTPLSLCDGADHAVYAYGINVPAGVNPQLNNSPKTLHCDAKLPAGIARRIST
                     PDIYGTWKFSSFYDHLPIDGAAVDAIPRGENLPDEPLLVLGPKKDKVYLVDGDVKRHI
                     PDPWAMLAWHFEWAAVQDVTDVQLAAWQDGPSLRVRPVVVENSEGELWLIDDEIKDTS
                     SAGAGGTGGSGATKDAGPEGSGGASAQAGSGGSKPSESLKNNWYDHDDGSSGCACGLT
                     RSPGAGWLWSGLFGFAVLVGARRRRASGF"
     gene            complement(4301..5971)
                     /gene="sppA"
                     /locus_tag="PK710_08050"
     CDS             complement(4301..5971)
                     /gene="sppA"
                     /locus_tag="PK710_08050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOT09707.1"
                     /translation="MHALLSLLINIFRLIILPWLVFKRWRGIPKGMWVTLVADGSVRP
                     IGPARRFWEAPDRSVDLHAVHRAAALASRDKRITGLFVELRGFQGGSASALALRNALF
                     AWKAAGKSVVVHLPQGGGTRPLMAASAADRIVASPGSEIAPMGFAIEAPYVKGALAHL
                     GLSAEVMAQGRYKTAGEFLVDDTMSAPQQEQVNRLLDVTWESLLRTLSEGRSVTSEQA
                     KQWVDNGPWLASEAKELGIIDETLDMEDLLHELGTQEDIPPALPVARYLRHHKPLFRP
                     LFRRPFIAVVQVQGTIVGDSPSPWLPVAIDEQIGEALTMARQDPHVKGVILFVDSRGG
                     SASASERIFREVRKLGEQKPVVACLGDTAASGGYMVAAGAHAIVCEPTTITGSIGVVS
                     ARVIADQLLERVGIRMQTVKRGAHADMMTVSRGFEEGERASMERLMKKVYEQFVQAVA
                     AGRGLDIEKVEALAQGRIWSGVDAAQHGLVDRLGGFEDALEEVRHRLGPGGERLEPVW
                     VGPQQGPSWLGLLREKGMQMGGLGDLVTLSSLKQERVWAFCPWHIVDE"
     gene            5970..6137
                     /locus_tag="PK710_08055"
     CDS             5970..6137
                     /locus_tag="PK710_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09708.1"
                     /translation="MTERVVVQPFAPVSHPVSAACSMGSPERPPLGVRFRRLKVKQSK
                     YNGIAPPRRAE"
     gene            6316..7983
                     /locus_tag="PK710_08060"
     CDS             6316..7983
                     /locus_tag="PK710_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09709.1"
                     /translation="MNPPGQRSRAKDSKKAPEVEWFGGITLMPDYVFDAGESFRPEIL
                     LWVSNTGEIVGTLVGKPNEVIVGASESLQQAIEMPMFGELHRPTRVQVSSPELAEVLR
                     AGHPDIEIVCAPTPGLDDMFQLLFENMNKFNDIEPSYLTSGVTPQAMESLFKTAAMLY
                     RVKPWKIVPDDQTLFSVTIEQLDVRDWVLSVIGQAGQHMGLVLFSSIEDFESFLETGQ
                     HAELGPPSKTAPHIFFGYERRAKLDPAMQKEISDHQWEVASSQAYPMLGAVDEDYVSR
                     PLTSREVTITDTIAQALTLMLADKKTLRKAWKDEEPLVRTSTVTTPAGEIEITMSTPI
                     VFVPVKFDSPQGILQKLASLTVVGDELDYESRADLEEELVRLFADSPEAEPIEDISAC
                     QIVMDMAADYLGHTIATLQADDLHTVLFDIIPRKFSIEASYAPWLIEVTRAFYAFLKR
                     EYGLKQADACLKVLEGDASERLEAALSDSSNFGIAKSFLMAGAEAGFDMQSEEGINAW
                     FAAVQNGTANFSPSVPALGRQSSASKASDKAKKNKRKAIRKARRKNR"
     gene            complement(8318..9238)
                     /locus_tag="PK710_08065"
     CDS             complement(8318..9238)
                     /locus_tag="PK710_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09710.1"
                     /translation="MDRNPPNLEIPVFVATSAQHVPAQCVRFVSVDGSVPGAAVTWDH
                     HLTGEEINLDAMPARFDLTGFDGVGTTLADTDAVASVVAVLFGGKANLPVAMLDLLSS
                     ASYFCDHLIPHPRLPAEVNERGRGVHAHITWSLHSRPDAFGAVCRQLATLIFEGKELP
                     SDTTRHDAQRALARALDAEGRIWQVGKVGLVDLRGREPVDPEALYERVSCPVSVLLSE
                     HPQGGLRYTVGVNPLCKDRPTSLHRALELLAIAEHACGAPSLSPVPKPGSENWGGRAT
                     VFGSPWNYGSRLSVEEVIRWVTEGIEKPTG"
     gene            complement(9412..9750)
                     /locus_tag="PK710_08070"
     CDS             complement(9412..9750)
                     /locus_tag="PK710_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09711.1"
                     /translation="MAADTRIDVAVTHHFATRAAQRGLSPRLLHFILSYGTEFPAHGA
                     VSFTVRARDLPDDLRDLPEVELAKDWIVVMAENTLITCYHRPRASAFLRRKNKLPSWA
                     RPCRTDALRS"
     gene            complement(10109..11878)
                     /locus_tag="PK710_08075"
     CDS             complement(10109..11878)
                     /locus_tag="PK710_08075"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOT09712.1"
                     /translation="MNKERSFSASLAYIHSRKPEWFAMISPSKPTVEEQRELPSNLDY
                     FKLETTNEEVVQVFADLTVRTWQCTEDKYKAQAISLSPSDGASFSLSPESKAYIAPPY
                     DAELLEGWDNHTPLILKWYRDVSQKVLEKEPTIQMAGMLYGFYAFPPPSPKDILPDPA
                     NFSVVYVDRAQSYSHLFEQTSKLIDLMANGWTGITKRGWAWGYPTRFLGLAPTAGTES
                     KFTKPAATSNILTPSGVDILGRLVKNHAKMDLDGIVYKGESAFANTGLQTYLTGRLLW
                     DPTLDAEVERVDWTRRMFGEDTAVFIRIIDDRVTEWFRSCLIANGSAWPAELSALQCL
                     YVPNFPELEDLVLQAQAQSRTAIQQERFQMFLANVTIMRWRFDKAGLTKNIAPTKLDT
                     SDDNIDAIFAVDDPNISRYMGYPAGGGYRDTHVYHAEFVEDGVIDVRESAGYWANSVL
                     IIPRQDGEITIDVDRAEQNGLFTVGYGLFEIANPEPSGLHKLVPPTLLRKGRSIKFDG
                     KKGSYYVLSLGYSQDNPFKITINNARAFKYAAYDGRFATRTLRVNGPSEAPLIIYHDP
                     AQTEDFTMTDDADNMRVSLSYEP"
BASE COUNT         2614 a         3527 c         3262 g         2483 t
ORIGIN      
        1 gcgtctgctg ggtcgaaaga cgttcgccgt aaatcgcctg atgcacggta ttcgagaagt
       61 attgctcacg ctcgtcggca taataagagt tttgattgcc gatgcccgtg gtggggatga
      121 aggcaagaac accgacgcga agccaatcga tccccccgga aatggtagcg cccactgtaa
      181 atcccgaaaa accaggggct cgggattcag attcgcgtcg tgtgagccgg tagcgcgaag
      241 ggattgccgg cgacgaggcg ccgaggtccg gcaagtcata accagcagga cgatcggcca
      301 gtcggacctg cagtcggttc gcggcaccca caaatcccac ggatacgccc ggctgaccca
      361 aggtcatccc tgccggattg gcgtgagcag cgtaggaatc gtcgagcagg gccgcaccgg
      421 cacccgccat ggccgccgag cgggagcccg cgccaaatac cgtgagttcg tctgcaaagg
      481 cgggccctgc ccaaaacatc agcaaggcta ctccgtatac tgccaggatt ctcatggctt
      541 tcatggggat acgagacacg ttattcacag aaaatcggct gcgcaagata acgccaacgc
      601 cccgttcgtg caacaccccg acgccgcgaa atcgagtcta ttgcccaccg atccatccgt
      661 ccatcgcccg cacggattcg ccaggcggat ggaattgtcg cttggacttt caaaattctc
      721 gtgattctcc cggtccgtgg cgagccaatc cctcgaaagc cgcctcccca acggctccaa
      781 cgcgtactcg aaagcgtgat ggccgaaagg tcgtcaaagg taaggaccaa attcgcatcc
      841 atgcgccaaa ccggacagaa aataccggtg cagcgcgcgg ccctgttcat cggaggaggc
      901 aagatcaaag gagactatcg tctgaccatc accgtcccgc acggataatt cgatgcgtgg
      961 cgccagtttg gaatcccccg gcgcggcctc tttgccctgc aatgcccgaa cccgttgccc
     1021 caaggtttgc cgccattgtt caggaatcgc ctttgattcc aatatcttag cctccgacgg
     1081 gggcgtcgta tcatgagata tttccacacg atggttcggt aatagcgcgt cggatggcat
     1141 aagcggcgcc ggactcaaat cccaaccatg ggcttcgaca cgaagcgaat cggcggatat
     1201 gccctgttga acgagccaat gtttgacggc ttccgcccgc tgacgggaca attccagggc
     1261 ggcctgcgcg ctctgcgctt catcatggtc ggcaaaccca tgcagaacaa ccgcgggttt
     1321 ggtgccttgt ttggcccctt tccaaagcgc gacgatccga gtcccttccg tggaaaacgc
     1381 gctatgatcg agtgttgcgc tgcgggattc gaaagtaatt ccaccgattt gccttcgacg
     1441 ggcctgaaag cagcgcgagg cggaagcatc cagcagcact tcgtccacga gccattcgac
     1501 agggcctgct tggaccatcc gataatgaac ggaaacagga acgacctcgg gcgaaaccgg
     1561 cttcggcgag gatgaggagc cggccgaaga aggggccggt gctatcggcc gaggcggtgg
     1621 cgacgaagaa caggccattg cgatggtagc agccaaaaac agggtccagc gagcatccac
     1681 gatcatgcct cgttcaggtg ggttggtttt cccaagcctg tcattggaac ggaaggaaaa
     1741 gcaagtccta tggacatcga cgggctgcct tggcctctcg tcgtcgtggc cgattcacgg
     1801 ggaagttcga cacgcccgag cgacatggcg aggagtcaaa ggcgcggcga cagtccatca
     1861 gaaaccggag gctcgtctcc tacgcgcccc gaccagcacc gcaaatccga aaaggccaga
     1921 ccataaccag ccagcgcccg gggaacgcgt caagccacaa gcacaacccg agcttccatc
     1981 gtcatggtca taccagttgt ttttcagcga ctccgatggt ttgctccctc cacttccggc
     2041 ttgcgcactc gcgccaccgc tgccctccgg ccccgcatcc ttcgttgccc cgcttccccc
     2101 agtaccgcct gctcctgccg atgacgtatc cttgatttca tcgtcgatca accatagctc
     2161 gccttcggaa ttctccacca cgacaggacg gactctcaga cttgggccat cttgccaggc
     2221 cgccaactgc acatcggtca cgtcttgaac cgcagcccat tcgaagtgcc aggcgagcat
     2281 cgcccaaggg tctgggatat gacgcttcac atcgccgtcg acgagataca ccttgtcttt
     2341 tttgggcccc agaaccaaca gcggctcgtc gggcaggttt tcgccgcgcg gaatggcatc
     2401 gacagccgcg ccatcgatcg gtaagtggtc gtaaaaactt gagaatttcc atgtcccgta
     2461 gatgtcgggc gtggagattc gtcgagcgat cccggcggga agctttgcat cgcagtggag
     2521 cgtcttggga gagttgttca actggggatt gactccggct ggaacgttga ttccgtacgc
     2581 gtaaacggca tggtctgcgc catcgcatag gctcaggggc gtgtgcatcc gaaaaccgtg
     2641 attgcaggag cccaccaccg aacataagtc atctctttcg acattggcta gtaccgacac
     2701 cccatgggct ccatcacttc ctgccggcgc gttgaagtag atatgcacat cgattgcggt
     2761 ttcaccggca tcttcatcga aggcccagcc cgaaatcgat tggtcgtcgg catggtccaa
     2821 cgagccccac ggaggctggt tcgagcaaaa cgccttgggc gagccaccgc cccaccaggt
     2881 ccaatgccaa tcctcgccca ccgaggcgcc ttcatcgtta tcccaaccga aactcggggc
     2941 attggcaaaa atccacgccc cccgtccccc tcccatatca aagtcaatcg catgcccgct
     3001 ttggtgattg ctatatcctg gcggagcggc tggattacaa ttgttacaac tgcaggtcgt
     3061 atagcaatga tagaaatagg cctgttcgtc ccaggttcga aacgcatcgt tgatttttag
     3121 aggaacgccc gcgttttccg cggcctccat catcgtcaac agcgcatccg ccgtcttgac
     3181 ctccacggga aaaccttgca ccgtgaccac ttgaatcgga aacgactggc cgttggtata
     3241 gcccgtctct tcggttacgg tgcaatcgta ggataaagac catgtcgact gcgccacggt
     3301 ctgctcctcc gattccaccc cataagcagg agaactacac ctcgtccccg ccgcggccaa
     3361 atagattaag gttacgctct tccatacctt tgccatcgag cagggagcct tcatcgataa
     3421 cctcagattc gccttcttgg gtgggaatgg gcaagttcta ttactttggc ttgaaagctg
     3481 aaacctatca acaatcatgc caaatacacg atattctact gcattccttg ttccctattc
     3541 gtctgacaac gccatccccc ggcgcctgcg gcatctcctg ccccatctcc ccatgggttc
     3601 ttggcctttc cgtgattctc gccgtacgga aaatgggaag atggcctttt cccctgctgg
     3661 cccaatcgtc cagaatggat agccttcgac ggctaatcca atttggctta tatttcctat
     3721 cgctaacacc atttaccgcg gattcacagg cgaacgaatc atgatttcgg aataccacgc
     3781 atcgctcgtc atgggaaaac gcgctggttt gacgccgtct ccgccaagca agataaaacc
     3841 actcggccga tggttatcga agctttcagg tccagactca gggggccgtg gctctcaaga
     3901 tcataggcaa cggcaagtgc ccgagggcat ccttttcatg ccagaaatcg taaggggatg
     3961 gctcgcggcc ccccatggca gcatgagcca cggccatgat cgcccggatc ccaggtgaga
     4021 gatgggccat ccccatggcg gccactgaaa acgccttacg tgggtaacgc agaggagagt
     4081 tggtgtgaat gccctaggca actacaggtt acccgacatc aagacgccaa acgtctttcg
     4141 ctcgcaaagc cattatcgct tgcccgcgct gcctcgacac ccttttgctg ctgagccgta
     4201 aaggtattaa ccgcgaatcg ccgggggatg ccgttgccgc aaataccata atatgttgaa
     4261 ttataagaag tttttgtaat cccaacgcga tcgtctttgc tcattcatcc acgatatgcc
     4321 aggggcaaaa ggcccatact ctttcctgtt tcagcgatga taacgtaaca agatccccca
     4381 ggcctcccat ctgcatccct ttttcccgca gcaatcccag ccaggacggc ccctgttgcg
     4441 gtcccaccca gacgggttcc agacgctcgc ctcccggccc caatcgatgc cgaacctcct
     4501 cgagcgcatc ttcgaagccg cccaatcggt ccacgagtcc gtgctgcgcg gcatccaccc
     4561 cagaccatat acgtccctgg gcgagcgcct cgactttttc gatgtccagg ccccttcccg
     4621 ctgccacggc ttgcacaaat tgctcataaa cctttttcat gagcctttcc atgctcgccc
     4681 gttccccttc ttcgaaacct cgggatacgg tcatcatatc cgcatgcgcg cctcgtttca
     4741 ccgtttgcat gcgaatcccg acccgctcga gcagttggtc ggcgatcacg cgggctgaga
     4801 ccacgccgat cgaaccggta atggtcgtgg gttcgcaaac gatggcatga gcaccagccg
     4861 caaccatata tccaccactg gccgccgtat ctcccaaaca ggccacgacc ggcttttgct
     4921 cgccaagctt tcggacttcc cgaaaaatcc gttccgacgc actggcgctg ccacccctcg
     4981 aatccacgaa caaaatcacc cctttcacat gagggtcttg ccgagccatc gtcaacgcct
     5041 cgccgatctg ctcgtctatg gctaccggca accaggggga tggagaatca ccgacaatcg
     5101 tgccctgcac ttgcactacg gcgatgaaag ggcgccgaaa cagcgggcga aatagcggtt
     5161 tatggtgccg aaggtagcgc gccaccggga gggccggcgg aatatcctct tgcgttccca
     5221 gttcgtgcag caggtcttcc atgtccaggg tttcatcgat gatccccaat tccttggcct
     5281 ccgaagcaag ccagggcccg ttatcgaccc attgttttgc ctgctcggag gtgaccgaac
     5341 gaccttccga caaggttcgg aggagagatt cccaggtcac gtcgagcaat cggttgactt
     5401 gctcttgttg cggcgcgctc atggtatcgt cgaccaaaaa ctctcccgcg gttttatagc
     5461 gcccctgggc catgacttcc gcggaaagac ctaggtgggc cagagcgcct ttgacatacg
     5521 gcgcctcgat ggcaaaaccc atgggggcga tctcgctgcc tggagaggca acgatgcggt
     5581 cggcagccga cgctgccatc aagggcctgg tcccgcctcc ctgaggcaga tggaccacca
     5641 cggactttcc cgcggctttc cacgcaaaaa gcgcgtttcg aagggcgagg gccgaagcgc
     5701 ttcccccttg gaaaccgcga agttccacga aaagccccgt aatcctttta tccctggacg
     5761 ccaacgccgc ggcacgatgc accgcgtgca aatccacgga ccggtcagga gcctcccaga
     5821 aacgccgggc cggaccgatg ggacgaacgg aaccatcggc cacgagcgtg acccacatcc
     5881 cctttgggat gccacgccaa cgcttgaaaa ccagccaggg aagaataatc aagcgaaaaa
     5941 tattgatgag aagcgaaagg agagcatgca tgacagaacg cgttgtagta cagcccttcg
     6001 cgcctgtctc ccatccggtg agcgcagcat gctcgatggg ttcgccggag cggccgccct
     6061 tgggagtccg atttcggcga ctgaaggtca aacaaagcaa atacaacggc attgcgcccc
     6121 ctcgaagagc ggagtagcgt ttcgataagt gcaatccacg gctcggttca gaggtgtgaa
     6181 agaagatgat cgaaggcccg tacaagacct atttcgtcgc tttccacacc ttcgaacctg
     6241 ccctcgaaga cagtcgccag tggcatggca tcgtcccatc ccaatcgcca acgcctatcc
     6301 atcgaggaaa tagcaatgaa tccccccggc cagcgctctc gtgcaaagga ctccaagaaa
     6361 gctcccgaag tcgaatggtt cggcggcatc accctcatgc ccgattatgt cttcgacgcg
     6421 ggcgagtcct ttcgaccgga gattttgctc tgggtcagta acaccggtga gattgttggc
     6481 acgctcgtag gcaagcccaa cgaagtcatc gtgggagcga gcgagagcct acaacaggcc
     6541 atcgaaatgc caatgtttgg cgagcttcat cgccccacgc gggtgcaggt gtcatcaccg
     6601 gagctggccg aggtgctacg cgcggggcat cccgatatcg agatagtatg cgcgccaacg
     6661 cccggcctgg acgacatgtt ccagttgtta ttcgaaaaca tgaacaaatt caatgatatc
     6721 gagccttcgt acctgacgtc gggtgtcaca ccgcaagcca tggagtcgtt attcaagacc
     6781 gcggcgatgc tgtaccgggt caaaccgtgg aaaatcgtcc ccgacgatca aacccttttc
     6841 tcggtgacca tcgaacagct cgacgtgcgc gactgggtgt tatccgtgat cggacaggcc
     6901 ggccaacaca tgggactcgt attgttctcg agcatcgaag actttgagtc tttcctcgaa
     6961 acgggacagc acgccgagct gggtccaccc tcaaaaacag caccccacat cttttttggc
     7021 tatgaacgtc gcgcaaaact cgatcccgcg atgcaaaaag aaatctccga ccaccaatgg
     7081 gaagtcgcca gttcacaagc ctatcccatg ctgggcgcag tcgatgaaga ctatgtttcc
     7141 cggcccctca cgagccggga agtcaccatc accgacacca tcgcccaagc gctgaccctg
     7201 atgctggccg acaaaaaaac gttgcgaaaa gcatggaaag atgaagagcc cctcgttcgc
     7261 acgtcgactg tcacgacccc cgcaggcgag atcgaaatca cgatgagcac tcccatcgtg
     7321 ttcgtgcccg tgaaattcga ttcgccccag ggtattctcc aaaaactggc atccctcacc
     7381 gtcgttggtg atgaactcga ctacgagtcc cgtgcagacc tcgaagaaga gttggtacgg
     7441 ctttttgccg attctcccga ggcggagccg atcgaagata tttccgcctg ccaaatcgtc
     7501 atggatatgg ccgccgatta cctgggacat accattgcca cgctgcaagc cgacgatctg
     7561 cacaccgtgc tattcgacat cattccacgc aagtttagca tcgaggcctc gtacgcgccg
     7621 tggctcatcg aagtgacccg ggcgttctac gcctttctga aaagggagta cggcctcaaa
     7681 caggccgacg cttgtctgaa agtgcttgaa ggggacgcca gcgaaagact cgaagcagcc
     7741 ctgtcggaca gcagcaactt cggcatcgcc aagtcgttcc tcatggcagg agccgaggcc
     7801 ggcttcgaca tgcagtccga ggaagggatc aatgcctggt ttgcggccgt ccaaaacgga
     7861 acagcgaatt tttcgccctc tgttccggct ttaggccgcc agtcatccgc ttccaaagcg
     7921 tcagacaagg caaaaaagaa caaacgcaag gccatccgaa aagcgcgcag aaagaacagg
     7981 tagcccccgg ttgatgagaa tcaagcgata ttgcaagcgt ttttcttgca ctcgtggcta
     8041 ttccagcctt tggctcgacc atcggcttcg ttttctcacg gcgtgtcttg cacaaggatc
     8101 ggtttcggcc cgttgcagaa aaagacgagc tactttgata agatggacga cttttgggtc
     8161 tggcaacatg gatgcaccag gtcagcatca aaaagccgat agcgcatcgc ctcccacaac
     8221 acatataacc atttgaaatt acacatttaa atcatttttt cccgccacag gggttcgcga
     8281 ctgcggtcac ggcccgatgc gccttgtctt ttgctcttca accggtgggc ttctctatcc
     8341 cctctgttac ccagcggatc acctcctcca ccgagagccg cgacccgtag ttccagggcg
     8401 aaccgaaaac cgtggcccga ccaccccagt tttccgaacc tggcttggga accggggaaa
     8461 gcgatggtgc cccgcaggca tgctcggcga tcgcaagaag ttcgagcgcc cgatgcagcg
     8521 acgtcggtcg gtccttgcac agcgggttca cgcctaccgt atagcgaagt cccccctgcg
     8581 gatgttctga tagcagcacg gatacgggac aactcacccg ttcgtacaag gcttcgggat
     8641 cgacaggctc tcgccctcgc aaatccacca gaccgacctt gcccacctgc cagattcgtc
     8701 cctcggcatc gagggcacga gcaagcgccc gctgcgcgtc atgccgtgtg gtgtccgacg
     8761 gcagttcttt gccttcgaaa atcaaggtgg cgagctgtcg acacacggct ccaaacgcat
     8821 ccggtctcga atgaagactc catgtgatat gcgcatgcac ccctcgacct cgttcattga
     8881 cttccgcagg gaggcgcggg tgagggatca gatggtcgca aaaatacgag gcggaggaaa
     8941 gcaggtcgag catcgcgaca ggcaggttcg ccttaccgcc aaaaagcacc gctaccacgc
     9001 tggccacggc atcggtatcc gcgagcgtag tccccacccc gtcgaaacct gtcagatcga
     9061 atcgggcggg catggcatcg aggttgatct cctctcccgt cagatggtgg tcccaggtta
     9121 cggccgctcc cggaacggat ccatccacgc tgacgaatcg aacgcattgg gcggggacgt
     9181 gctgcgcaga cgtggctaca aacacgggaa tttcgaggtt cgggggattc ctatccattc
     9241 gggtactcca tcagccgtgg ttgcgacgtg tttgaaagcc ctggtatggt agcatgtatg
     9301 ccacggcggg gctttggtgg gtcagcctcg ttccttgggc gccatcggaa caaagccaga
     9361 gatggaaaag agctgcttcg agttcgcgcg gcacccgggg attcgtgggg ctcatgagcg
     9421 taacgcatcg gtgcggcacg gtcgcgccca cgagggaagc ttatttttgc gccggagaaa
     9481 agcagacgcc cgagggcgat gatagcaggt gatcagggtg ttttcggcca ttactacaat
     9541 ccaatctttg gcgagttcga cctctggcag gtcccgcagg tcgtcgggga gatcgcgcgc
     9601 acggacggtg aagctgacgg cgccatgggc ggggaattcc gttccatagg agagaatgaa
     9661 gtgtaaaaga cgaggcgaga ggccgcgttg ggcggctcgg gtggcaaagt ggtgggtaac
     9721 ggcgacgtca atcctggtat cggctgccat gtttctacct ccttgatcag caagacgctg
     9781 gtctggaggc agttctgaca gaaaaagcgc tgccgaccgt tccgagggga ccgagtcacg
     9841 cgcttgcgtg tctgggtgct tgtggtgcct attgtccttt ctcgaaaacg gtctatctcg
     9901 gcctgcccgc gtgccagggg ctgacgcgag cctgagccat cccctcattt ttccaggagt
     9961 atttcccttg ttggcgactg ccggaaagtc gtccccatca ccactcggcc agaatcgttc
    10021 aggttcatga cgaggtcaaa ctcaaggttc cggggtccac gttcacgttg ttgttggtaa
    10081 ggaaaaatga ggggatcttt caggtttttc acggctcgta cgagaggctc acccgcatgt
    10141 tgtcggcgtc gtctgtcatt gtgaagtctt cggtttgggc cggatcgtga tagatgatga
    10201 gcggtgcctc gctcggacca ttgacacgaa gggttcgcgt tgcaaagcgt ccatcgtaag
    10261 ctgcatattt gaacgcacgt gcgttattga tggttatttt gaaggggtta tcctgcgagt
    10321 agccaagcga tagcacgtaa tagctaccct ttttcccatc gaatttgatg gaacgccctt
    10381 ttcgaagcaa tgtgggcggc accaatttgt gcaaccccga tggctcagga ttggcgattt
    10441 cgaaaaggcc atatccgacc gtgaacagtc cgttttgttc cgcacggtcg acgtcgatcg
    10501 tgatctcgcc atcctgcctc gggatgatga gcacggaatt tgcccaatac cctgcagatt
    10561 cccgaacgtc gatgacaccg tcttctacga attctgcgtg gtatacgtgt gtgtcacggt
    10621 atccaccgcc tgctgggtag cccatgtaac gactgatatt gggatcatcg accgcgaaga
    10681 tggcgtcgat gttatcgtcg ctcgtatcga gttttgtagg agcaatgttc ttggtgagac
    10741 ctgctttgtc aaaacgccat ctcatgatgg tgacgttggc caaaaacatt tgaaagcgct
    10801 cttgttggat ggcggtgcgc gactgtgctt gcgcttgcag gaccaggtct tccaactcgg
    10861 ggaagtttgg tacgtacagg cattggaggg ctgaaagctc cgcgggccat gccgagccgt
    10921 tggctattag gcaacttcgg aaccattcgg tgactcgatc gtcgatgatt cgaatgaata
    10981 ccgccgtatc ctctccgaac atgcgacgag tccagtcgac ccgttcaact tctgcgtcga
    11041 gggtgggatc ccaaagaagt cgcccggtca aataggtttg aagtccggtg tttgcaaagg
    11101 ccgattcacc tttgtacacg attccatcga gatccatctt cgcatggttt ttgacgagtc
    11161 ttccgaggat atcgacaccg gaaggcgtaa ggatattgct cgtagcggct ggcttggtga
    11221 acttgctttc tgttcctgcg gtgggcgcca aaccgaggaa acgggttgga tacccccaag
    11281 cccaccctcg tttggtgatg ccggtccaac catttgccat caagtcgatg agcttgctcg
    11341 tttgttcaaa taagtgagag taggactggg cccggtccac atagaccact gaaaagttcg
    11401 ctggatcagg caagatgtct ttcggcgacg gggggggaaa agcgtaaaac ccgtagagca
    11461 ttcccgccat ttggatggtg ggttcttttt ccagcacttt ttgggaaacg tctcgatacc
    11521 acttgagaat gaggggcgtg tgattgtccc aaccttcgag cagttcggca tcgtaaggag
    11581 gtgcaatata ggccttggat tccgggctta ggctgaagga ggcaccgtcg gaaggcgaaa
    11641 gggagatggc ctgggcctta tatttatcct ccgtacattg ccacgtgcga accgtgagat
    11701 cggcaaatac ctgaacgact tcctcattcg tggtttccag cttgaagtaa tcgaggttcg
    11761 aggggagttc gcgctgttcc tcaaccgtgg gtttggaagg cgaaatcatg gcaaaccatt
    11821 ctggcttgcg actatgaatg tatgcaaggg acgcggagaa gctccgttct ttattcaact
    11881 catcac
//
LOCUS       DAOSOR010000109        11873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5345_length_11873_cov_4.292858, whole genome shotgun sequence.
ACCESSION   DAOSOR010000109 DAOSOR010000000
VERSION     DAOSOR010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11873
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5345_length_11873_cov_4.292858"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PK710_08080"
     CDS             <1..470
                     /locus_tag="PK710_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09713.1"
                     /translation="QRLQEPAKRQFLAQRPNPSHPLDVELQGQLKALGWDMVDPEGRV
                     VDSVIAGRKYRLRLYYEVIGRVGRDWECFIHIDGHGRRFNGDHLPMDKKYPMTLWQVG
                     DVLVDDYPIQLEPNFTPGGYGLYYGFFVGNTRLKVTRGKHNDDRIEGGTIQVR"
     gene            complement(557..2338)
                     /locus_tag="PK710_08085"
     CDS             complement(557..2338)
                     /locus_tag="PK710_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09714.1"
                     /translation="MNRRFGVACAMLALSMSCARHDPHQVSGIGVRLNSPRTLLDGVD
                     TVTLSIVDAHELGVVCSENSGLVTVGELKSGLLSIDLQAQEEVVKVPLSRTMPNGAPC
                     PNQGVFCSDEITLPTDPSKLLVFQAEGRAGTTPVAVGCTTAAVNSDPFYVNLSMFRYV
                     KPAICGNGSLEVGEQCERGEINCDDLCRTVEFLISTDHEGPSNQRIENAPPGSKQGVR
                     MAWSHAPQAANPSPLHLVFGDTNYGKTGTGPEINYRQLGQHAEPIEYPPLLSSQIRLP
                     LLGAKTPGFDQRPRTQAAAAIGLMSDGSFVVAYEDDRLSTGPNISFTPISMDASLPRS
                     DEVSINTLGVDASGSPAVAGGPTDRALVAWVDQAGRRIRARIWAVTGWISSSETTLSA
                     QEGDSPRIAGWNEGWVVVWHGRSKQDNDDIMAVRISQTGEVGAPFSVNTETKDVQQQP
                     DVAALPTGEVAVVWNSAGRIFLQRFDASGQPIAGDQSAPVNDDSAMGENPAIAASPLA
                     AGFFAISWQTAAGIRARLIDRSGDYLPNAVDGQTGSFLVNRDDVNGMRSHPSVAIGGA
                     GHIVFAWEDSAADHSGIYARRFPLPVR"
     gene            complement(2385..3446)
                     /locus_tag="PK710_08090"
     CDS             complement(2385..3446)
                     /locus_tag="PK710_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09715.1"
                     /translation="MDCGQFDQIVMDLLYGETSESSSVEAKRHVDQCERCAAVLADLR
                     TAKSTLNLPMEQAPESIEPKVLEAARQYQKEIPWPRRLGRWISWVGGYAMRPQLAMAA
                     LLLLMLGSSLLLIRARPGAEQVDVVRVVEQGVPQPETLEGSHAIEPHQLPPSEDEPLD
                     RRKDSRNGTVEPPDTARTKTSKAEADAPSTAPTSSTAPPAADGFSDAMAMYRAKDYAN
                     AYRAFDQVVLKGGPNAPAAALLAAKSVRASSGCAHALPRFESVSSRYAGSSEAIEARW
                     ETASCARIIGDLTRARLIYRDLAQIEGLRERAENELARISTGSTPPRIPPPSTPDVGR
                     TDVPGNPTPPPSTSSPQDQ"
     gene            complement(3470..3994)
                     /locus_tag="PK710_08095"
     CDS             complement(3470..3994)
                     /locus_tag="PK710_08095"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOT09716.1"
                     /translation="MVARGRQDVADEVLMLRFQRGDVSAFSLLVQRHQTPLFNYILRM
                     VHSREVAEPLVQQALIDMVRGSPGFKHDGAFGSWAYGIARRICLPYSTKAQETSVISD
                     NPQPGTDPILAALEALPTEQREVFLLKKIARLSFKDISGMTDTDEQTVKSRMRFVLDH
                     LQAVLHSVEEDASS"
     gene            4384..5130
                     /locus_tag="PK710_08100"
     CDS             4384..5130
                     /locus_tag="PK710_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09717.1"
                     /translation="MTLVLLLGAKPVGAQTPKETESAAASSSAAPASSSVPAASAPEA
                     TTAGPPDTMDGSTYSIRLRDLEQRIDELKEQIRRSHTRLSLLSDSILGGGSASARADI
                     TFDNEMSGAFRLTRAVFVLDGAVQYNRSDDTGALADQKTIPIYQGSIPPGDHTLQVVL
                     ELRGHGYGVFSYLRGYKFEVRSSHSFTITDGNALTLRTIAYEKGGVTTPLEQRPAVRF
                     IENVGSLDSRGTPASSPKPAAKATLSIGGK"
     gene            5132..7498
                     /locus_tag="PK710_08105"
     CDS             5132..7498
                     /locus_tag="PK710_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09718.1"
                     /translation="MHSRTRLVGMVAFFALTASVNGANAQNADEAYASAQRTITQYRN
                     GINTIQDAMARASRPEQTAEQRLADGNLLLLTRDFDRASVVFSQIVEKYRNNDTVYAE
                     ALFLLGETYYQSEQLTSARRVFRTIVEDKRSRRVQAFHPKALARLTDIAIRTRDIETL
                     EHLKQQLGEAPSNPLLYYAKGRVQLAQQDLSGAKASLSAVPAQSDFYPQARYLLGVIA
                     LREAKTALAASTETAKSPQESSKPYASAIEAFRQVTQLPASTKEHRHVVDLGWLAIGR
                     LHDETNQWPEAVEAYNHIDRQSPEFSNALFEVAATHMQMNDVVRAQRALEILMLVDPD
                     GERTAEASLLRGDLELRSGQYRKSLTTFEDIRSQFEPQLLRVSSFLSGSDDPAVFYDK
                     LVEDQLISAETGGNLPPLAIQWAREADDGPEAFAVVNEVVQTRMLLRTSEDALKKLHA
                     VMNSAGRSKAFPEYRAGQQTAIGAIHGITKARVVLANALDAQEPSSLSGEIGSVRQQR
                     RALQAQVLDLPVSSSDFQQRDNRAENRWNSASQKVQQLQLQIDTLNSVVNALQRVIRE
                     SASRGVVRDPASIRQFQEELGAHERELTGYQARLAELRQQAGQGRIASGFDDASVFDD
                     QAVREQYKQLLAKEVELASRGAAGNSAAQYAQRIAPLLRSADEVEARYQAALRDINHK
                     VDEKSKALLLSIAAEESKLGEFNAQLQQLDEEARLVVGHVAMRNFGLVRDRMRSIVMH
                     AEVGITEQAWETRESQLIRVRRLQSERARSERLLDEELREVLDDAMDE"
     gene            7542..11747
                     /locus_tag="PK710_08110"
     CDS             7542..11747
                     /locus_tag="PK710_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT09719.1"
                     /translation="MKRFRKTHVARTSALLLLAGLLTVTPATTVYSQPSSSTNSGVNP
                     ALERPLDDTLAEFFQSDGSLSMGGEHSAKASGRPAASPLPAPSREKLRALALLQREAD
                     TYQRDAKDFRDAITGIVRHHYEQQRRRTLSSLDSQILAERTELEKARAEAIQKLEAFI
                     EKYSGQTADPEATPNAMFRLAALYDERARAKNMTDIGRDDLKPSIALYKRIIKEFPQY
                     DELAAVYYYLGHALFDSERLEEAQQVWRALVCHNNYPYPVATDPRDPDKDIVGDVPQD
                     HDADYWVGWRHVFPTPENLTKQAKPNPRRAAADTTQWELSYTSPFPDTCTYLPQTVKP
                     GEDPRYVAEIWWKIGDWYFDEADPKAGPYTFNRAATAYQKSMQAASNEKGVLYGVSMY
                     KLAWTYFKQQRYEAATRKFVELLHYTDEVEKKTGDPGADFRSEAYTYIAGSLTYVDFA
                     GPQASEPFIPRSDVLDLESDPTIIERKMRIAIDRIQDPGLIPQDKPWTFHVYKALALE
                     YKELNQLHNRIELSEKMLEKWPMHREAPEIQAGIADTYDELTRTSREGTVERKENAAK
                     ALEARSKLAVYVGNSPWVNANRDDPEALQMAERLVHGGLQSAAAEHTNRARAFLQEAE
                     QMGDAAERNVQLEKTLAEFKLAERAWSAYLDQDENASDAYESRFWLADARHWIVYTKV
                     LAGQSPTIGDVLRARQSAVEVRDSNEDDRRLEVAAFYAVNLAFLVQNDRNRVWQETSG
                     AKGLEERNNLVFVGEGDNRKVVSDPIPPAIRFRNLSQEEYIRTVPEQHDINKRSPMFA
                     FQVAESFFFYGQFGEAKKRYEPIYQKECGKTDYGYRAWERLLTMSNIEGDVEQSTKLA
                     EAQKANSCAVSEEQKTAETLLINPTLQEAAYRDARKAFDEAQNMPDGPERNEKWRKAA
                     ALYRKALENAPDRNEAPEAAINGAIAYKQLGEYDKAIEMYELFIKRYGDEKTLVVLEK
                     GNPKASPPVAPNPEKYKERLTYLKQAHNALSEAYILFFDYRRAAEQYDTISQIERFDQ
                     KDRREAAKLALELYANMGDKGKTATLQKRFLSLGPTPQEKAEAEYIVARGEMNEWDER
                     GRDEGANRAARQRALTAMTAFHARNKNNRDAAKYVVRASYNAAKLQKVGGGNQYQDWY
                     KKTIAAFEQWKAVAPKKSDGQSSAVSTAEAAMAAEAEFSLLDQEIREKFDYDTGHHRY
                     KGTTVDVIRDYRADAADAKRYHDRLQRVIDVYISPEWAVAARSRQGSLYDSLRTGMYN
                     AREPALKLFSDKEEALLKKLEASDDPEHQDKADVFRTNRQNVWRQTRDKELTDADKVM
                     VTRYVEAFSLGRKYNISNPAIDRAVERLAFFTDVLGDAKLREYSQGVAGFTYTDRMFL
                     LSRPGIIEQPQIDPLPHPLPVVP"
BASE COUNT         2793 a         3375 c         3344 g         2361 t
ORIGIN      
        1 aacaacggtt gcaggaaccc gcgaaacgcc agttcctcgc acagcggccc aatccctccc
       61 acccactcga tgtggaattg caaggacagc tcaaagctct cggctgggat atggtcgatc
      121 cggaaggtcg cgtcgtcgac tccgtgatcg ccggtcgcaa gtatcgattg cgcttgtact
      181 atgaagtgat tggacgggtt ggccgtgatt gggagtgctt catccacatc gatggacatg
      241 gtcgtcgatt caatggcgat cacctgccca tggacaaaaa atacccaatg acgttatggc
      301 aagtcggcga tgtgctcgtc gacgactacc ccattcagct cgagcccaac ttcactcccg
      361 ggggatatgg cctgtactat ggttttttcg tgggcaacac gaggctgaag gtcacgcgag
      421 gcaagcataa cgacgatcgc atcgaaggtg ggaccattca agttcggtaa tgtcaagcgt
      481 aggttggcgc tgggaagtcc agccttcttg cggcaggcgc ttgtggtgga cgttcgcctt
      541 cgcggcgctc ttgccatcag cgaacgggca agggaaaccg tcgcgcgtat atcccgctat
      601 ggtcggctgc gctatcctcc catgcgaaaa cgatatggcc cgcaccaccg atcgcgaccg
      661 aaggatggga gcgcattccg ttcacatcgt cgcgattaac caaaaaggat cccgtctgac
      721 catccactgc attgggtaag taatcgccag atcgatcgat gagacgggcg cgaatccctg
      781 cggcagtctg ccacgatatc gcgaaaaaac cagcagccaa cggtgaagcc gcgatcgccg
      841 ggttttcgcc catcgcggaa tcatcgttga cgggcgcgga ctgatcgcca gcgatcggtt
      901 gccctgatgc atcaaaacgt tgcaagaaaa tacgtccggc gctgttccac accacggcaa
      961 cttctcccgt gggaagggca gcgacatcgg gttgttgctg cacatctttc gtctcggtat
     1021 tcacggaaaa cggtgctccc acttctccag tttgagaaat ccgcaccgcc atgatgtcgt
     1081 cgttgtcctg tttcgagcgg ccatgccaga ccaccaccca cccctcgttc caacccgcga
     1141 tacgagggga atcaccttct tgcgccgaca aggttgtttc cgaggaagag atccaacccg
     1201 tcacggccca tatccgagcc cgaattcgac gtcctgcctg gtcgacccaa gcgaccagcg
     1261 cccggtccgt gggtcctcca gccacggcgg gactgccaga agcatcgacg ccgagggtat
     1321 tgatggatac ttcatcgctt cgcggcagtg acgcatccat cgagatcggg gtaaacgaga
     1381 tattgggacc ggtgcttagg cggtcatctt cgtaagccac gacaaaagag ccgtcggaca
     1441 tgagcccgat cgcggcggcg gcctgggtgc gggggcgctg atcgaatcct ggagttttcg
     1501 ctcccagcaa cggcaagcgg atctgcgagg ataaaagcgg cgggtattcg atgggttcgg
     1561 cgtgttgccc aagctgtcga taattgatct ccggtcccgt ccctgttttg ccgtaattcg
     1621 tatcgccgaa aaccagatga aggggactgg gattggcggc ttggggcgca tgactccaag
     1681 ccatgcgaac gccttgttta cttcctgggg gagcgttttc gatgcgctgg ttcgatggcc
     1741 cctcatggtc ggtggagatt aagaactcga ccgttcggca cagatcgtcg caattgattt
     1801 cacccctttc gcattgctct cccacttcca gcgatccgtt tccacaaatc gctggtttca
     1861 cataacgaaa catcgataaa ttcacataaa acgggtcgga attgacggcc gctgtcgtac
     1921 atccaaccgc aacaggagtt gtccccgctc gtccttccgc ttgaaacaca aggagtttgg
     1981 aaggatcggt gggaagtgtg atttcatcgg agcaaaatac gccttggttc gggcaaggtg
     2041 caccgttggg catcgtgcgc gacagcggca ctttgacaac ttcttcttgc gcttggaggt
     2101 cgatggaaag gagccccgat ttcagttcac cgacggttac gaggcccgag ttttccgagc
     2161 aaaccacgcc aagctcgtgg gcatcaacga tagacagagt gaccgtatcg accccatcca
     2221 aaagtgtacg gggggaattg agtcgaacgc caatgccgga cacctgatgc ggatcatggc
     2281 gggcacagga catggaaagg gcgagcatgg cgcaagccac accgaaccga cgattcatgc
     2341 aggaagtcta gcgtgtggat gaatcgggtg aaagagcctt gccatcattg gtcctgcggg
     2401 gagctggtcg atgggggagg agttgggttg ccgggaacat ccgtccggcc gacatcgggg
     2461 gtcgagggtg gaggaatccg tggtggggtg cttccggtgg agatacgggc caattcgttt
     2521 tccgctcgtt cccgcaaccc ttcgatttgg gcgaggtccc tgtagatcaa tcgagcccgt
     2581 gtcaagtccc ctatgatcct cgcacaggaa gccgtctccc atctggcttc gatcgcttcg
     2641 gacgatccgg catagcgtga agaaaccgat tcgaagcgcg gaagcgcgtg ggcgcaaccg
     2701 gaactggcac gaacggattt ggcagccaat aacgcggccg cgggtgcatt ggggccgcct
     2761 ttcaaaacca cctgatcgaa agcgcggtac gcgttggcgt agtctttggc tcgatacatc
     2821 gccatggcat ccgagaatcc atcggctgcc ggtggtgcgg tggatgaggt tggggcggtc
     2881 gaaggggcgt cagcttcagc cttcgacgtc ttggtacgag ccgtgtccgg gggttccacg
     2941 gtaccatttc gactatcttt tcgacgatcc aacggttcat cttcggaagg aggcaattgg
     3001 tgcggctcga tcgcatggct tccttccaag gtttcgggtt ggggtactcc ttgctccacg
     3061 acccgcacta catccacttg ttccgcgcct ggacgagcac ggatcaggag cagactcgag
     3121 ccgagcatga gcaatagcaa cgcggccatg gcgagttgtg ggcgcatggc atatccgcca
     3181 acccatgaaa tccagcgacc gaggcgacgc ggccagggaa tctccttttg atactgtcga
     3241 gccgcttcga gaaccttggg ctcgatgctt tccggagctt gttccatggg gagattcagc
     3301 gttgacttgg cagttcgcag atccgccagt acggccgcgc atcgttcgca ttgatcgaca
     3361 tgacgttttg cctccacgga ggacgattcg cttgtttctc cgtagagcaa gtccatgacg
     3421 atttggtcga actgtccaca gtccattcgt tcatccgctc ggcagaatac tacgatgaag
     3481 catcttcttc gacagaatgc aagacggctt ggaggtggtc caagacgaat cgcattcggc
     3541 tttttacggt ttgctcatcg gtatcggtca tcccggagat atctttaaag gacaaacgcg
     3601 cgattttttt gagcaaaaac acctcgcgtt gttccgtagg gagggcttcg agggccgcga
     3661 ggatgggatc ggttccgggc tgaggattat cgctaattac ggaggtttct tgtgcttttg
     3721 tcgagtaagg aaggcatatt cgtcgagcga taccataagc ccacgagcca aaggcaccat
     3781 cgtgtttgaa cccgggggac ccacgaacca tgtcgatcaa ggcttgttgg accaggggtt
     3841 cggctacttc cctagaatgg accatccgca aaatatagtt gaaaagcggg gtttggtggc
     3901 gctgcaccaa caaagaaaag gcgctcacat ccccgcgttg gaaacgcagc atcaacactt
     3961 cgtccgccac atcctgtctt ccgcgagcga ccacgccaaa tcctcattcg gtcgtcagaa
     4021 aacacgaccg gaaggttgtg gttgatggtg cgattcgaag cgataactca tattattttc
     4081 cgtgggtccc tccgccggcc agggggttga aaaggacggg gttcggcctt catggtctgg
     4141 gtaacaagta aaatagtgtt ctatcggccg gaaaagcaac gaccatgtca cgatcgacag
     4201 caacggatag gacgattttg catcaacctc tgaacacggt tgaaaggtga gccctcccca
     4261 tttacattac gggggtctat cgaatatgcg ctcggccgac aacagtccga ggggctgctc
     4321 cgtcgaagat tggggtcaac aatgacacgg acaatcacgc gacttgctca aaccatagct
     4381 atcgtgacac ttgtgctttt gctcggggcc aaacctgtag gggcacaaac acccaaagaa
     4441 accgaaagcg ccgcggcatc ctcttcggct gccccagctt cgtcgtcagt acctgccgca
     4501 tcggccccag aagcaacaac tgccggcccc cccgacacca tggatgggtc cacctattcc
     4561 attcgcttgc gagacctgga acaacgtatc gatgaactca aagaacagat ccggcgcagc
     4621 catacccggc tctcgctcct ttccgattcg atccttggtg gtggttcggc aagcgcgcgg
     4681 gcggatatca cattcgacaa cgagatgtcg ggcgcttttc gattgacccg cgcagttttc
     4741 gtcctggatg gagccgtcca gtacaaccgc agcgacgata ccggggcgct agcggatcaa
     4801 aaaacgatcc ccatttacca agggtccatt cccccgggcg accacacgct gcaagtcgtc
     4861 cttgaactac gaggacacgg ctacggggtc ttttcctatc ttcgagggta caagttcgag
     4921 gtgcgctcta gccattcgtt tacgattacc gatggcaacg ccctcacctt gcgaaccatc
     4981 gcgtacgaaa aaggcggcgt aaccactccc ctggaacaac ggcccgccgt tcgcttcatc
     5041 gaaaacgtcg gttccctgga cagccgtggt actccagcct cttcgccgaa acccgcggcc
     5101 aaggcaaccc tgtcgattgg gggaaagtga catgcactcg cgaacgcgcc tcgtggggat
     5161 ggtcgctttc tttgccctca ccgcatcggt caatggggcg aatgcgcaga acgccgacga
     5221 ggcttacgcg tccgcgcagc gtaccatcac gcagtaccgc aacggtatca atacgattca
     5281 ggatgccatg gcgcgggcgt cacgccccga acaaacggcg gagcagcggc tggcggacgg
     5341 caatctacta cttctcacaa gagatttcga ccgggcaagt gtcgttttta gccaaattgt
     5401 cgagaaatac cgaaataacg atacggtata cgccgaagcg ctctttcttc ttggcgaaac
     5461 ctattaccaa tccgagcagc ttacctcggc gcggcgtgtg ttccgaacca ttgtggagga
     5521 caagcgctct cggcgtgtgc aggcgttcca ccccaaagcg ctcgcacgac tcacggacat
     5581 tgccatccgc actcgtgaca tcgagacgtt ggaacatctg aaacagcaat tgggggaggc
     5641 gccttccaat cctctcttgt actacgccaa gggccgcgtg cagctcgccc agcaggatct
     5701 gtccggcgcc aaagcatcct tatcggctgt cccggcgcaa agtgatttct acccccaagc
     5761 ccggtacttg ctcggggtca ttgcgctacg cgaagccaaa actgctctcg ctgcttctac
     5821 ggaaacggcg aaaagcccgc aagagtcctc gaaaccctac gcctccgcca tcgaagcttt
     5881 tcgccaggtg acgcagctac cggcatctac caaagagcac cgacacgtcg tcgatctcgg
     5941 ttggctcgcc atcggtcgtc tacacgacga aacgaatcag tggcccgaag ccgtcgaagc
     6001 ctataaccac atcgatcggc agtcaccgga gttcagcaat gcgctgttcg aagtggcagc
     6061 cacccacatg cagatgaacg acgttgttcg agcccaacga gcgctcgaaa tcctcatgct
     6121 tgtcgatccg gacggagaac gtaccgcgga agcctcactt ttgcgaggag acctcgagct
     6181 tcgctccggc caataccgaa aatcgctcac gaccttcgaa gacatccgct ctcaattcga
     6241 gccacaactc cttcgtgttt cctcctttct ctccggttcc gatgatccgg ccgtctttta
     6301 tgacaaactc gtggaagacc aactcatcag cgcggaaacg gggggcaatc taccgcctct
     6361 cgccatccaa tgggcgcgcg aggccgacga tggcccagaa gcttttgcgg tcgtcaacga
     6421 agtcgtccaa acccgaatgc tccttcgcac ttccgaagac gccttgaaaa agctccatgc
     6481 ggtcatgaat tcggcagggc gttccaaagc gttcccagag taccgcgccg gtcaacaaac
     6541 cgccatcggc gccatccatg gaatcaccaa agcccgcgtc gtgctcgcca atgctctcga
     6601 cgcgcaagag ccctcatcgc tttccggtga gatcggctcg gttcgccagc aacgtcgtgc
     6661 gctacaggcc caagtgctcg atcttcccgt ctcttcctcg gacttccaac aacgggacaa
     6721 tagagcggaa aatcggtgga attcggcatc ccaaaaagtg cagcagcttc aactgcaaat
     6781 cgacacgctc aactcggtcg tcaacgcttt gcaacgagtg attcgcgaaa gtgcttcacg
     6841 cggtgtcgtt cgcgatccag cttcgatccg tcaattccag gaagaattgg gcgctcatga
     6901 acgagagctt acggggtatc aggcgcgtct ggccgagctt cgacaacagg cgggtcaggg
     6961 gcgaattgcc tcaggcttcg atgatgcctc tgttttcgac gatcaggctg ttcgcgagca
     7021 gtacaagcag ttgttggcga aagaggtcga acttgcgtct cgaggagcgg caggcaattc
     7081 cgccgcccaa tacgcgcaaa gaattgcacc ccttttgcgt tccgccgacg aagtcgaggc
     7141 ccggtatcag gctgcgttgc gcgatatcaa tcataaggtc gatgaaaaat ccaaggcctt
     7201 attgctatcg attgccgccg aggagtccaa gcttggggag ttcaacgctc aactgcagca
     7261 gttggatgaa gaagcacggc tggtcgtagg ccatgtggcc atgcgcaact ttggtctcgt
     7321 tcgcgacagg atgcgcagca ttgtcatgca tgcggaagtc ggaattaccg aacaagcatg
     7381 ggaaacccga gaatcgcagc tcattcgcgt gcgaaggctc caatccgagc gagcacggtc
     7441 cgagcggtta ctcgatgagg aattgcgaga agtcctagat gacgcgatgg acgaatgaca
     7501 tcggtcaaac cgaacggtct gactttgtat ggagagaaag catgaagcgc tttcgaaaaa
     7561 cgcacgtggc tcgaacatcg gcgttgctct tgctcgcggg actgctcacg gtgacccctg
     7621 cgacgacggt ctattctcaa ccttcgtcat cgaccaattc aggagtgaat ccagccctcg
     7681 agcgtccgct cgacgatacc ttggcggagt tttttcaatc ggatggctcg ctctcgatgg
     7741 gaggcgagca cagtgcaaag gcatccggtc gtcccgcagc ttcaccgctg cccgcgccgt
     7801 cgagagaaaa actgcgagcg ctggcattgc ttcaacggga agccgatacc taccagcgcg
     7861 atgccaaaga cttccgagat gccatcacgg gcatcgtacg gcatcattac gaacagcagc
     7921 gtcgtcgcac cctatcctcg ttggactcac aaatccttgc ggagcgtacg gaactcgaga
     7981 aggcgcgagc cgaggccatt caaaagctcg aggcattcat cgaaaaatat tcggggcaga
     8041 cggcggatcc ggaagccacg cccaacgcga tgttccgctt ggccgcgtta tatgatgagc
     8101 gcgctcgtgc caaaaacatg acggatattg gaagagacga cctcaaaccg agcattgcgc
     8161 tttacaaacg catcatcaag gaattccctc aatacgacga acttgcggcg gtctactact
     8221 atttgggaca cgccttgttc gactcggagc ggctcgaaga agcgcagcag gtatggcggg
     8281 ctctggtatg tcacaacaac tatccgtatc ctgtcgccac ggacccgcgg gatccggaca
     8341 aagatatcgt cggtgatgtg ccacaagacc acgatgcgga ttattgggtc gggtggcgcc
     8401 atgtttttcc cacaccggaa aacttgacga agcaggcgaa gccgaaccca aggcgtgcgg
     8461 ccgctgatac cacgcaatgg gagctatcat acaccagccc cttcccagat acttgcacct
     8521 acctgcccca gaccgtcaag ccaggggagg atcctcgtta tgtagctgaa atttggtgga
     8581 aaatcggtga ttggtacttt gacgaggccg atccgaaagc gggcccgtat acgttcaatc
     8641 gagcggccac ggcttatcag aagtcgatgc aggcagcatc gaatgaaaag ggagtcttgt
     8701 acggcgtatc catgtacaag ctcgcatgga cgtacttcaa acaacagaga tatgaggcgg
     8761 ccactcgaaa attcgtcgag ttgttgcact ataccgacga ggtcgagaaa aaaaccggtg
     8821 atccaggagc agattttcgt tccgaggcgt acacgtatat tgcaggctcg ttgacgtatg
     8881 tggatttcgc aggtccacaa gccagcgagc cattcattcc tcggtcggat gttttagatc
     8941 tcgagtcgga tccgaccatc atcgaacgca agatgcggat cgcgatcgat cgaatccagg
     9001 atccgggatt gatcccgcag gacaagccgt ggacattcca tgtctacaag gcgcttgccc
     9061 tcgaatacaa agaactcaac cagcttcata atcgcatcga gttgtcggag aaaatgcttg
     9121 aaaaatggcc gatgcatcgg gaggccccgg agattcaagc aggtatcgcc gacacctacg
     9181 acgagctgac gcgcacctcc cgggaaggca cggtcgaacg caaggaaaac gcggccaagg
     9241 ctttggaagc gcgaagcaaa ctcgccgttt atgttggtaa ttcaccgtgg gtcaatgcca
     9301 atcgggatga tccggaagcc ttacaaatgg cagaacgcct ggtgcatggc gggctgcaaa
     9361 gcgccgccgc tgagcatacc aaccgcgctc gcgccttcct gcaagaagcc gagcagatgg
     9421 gggatgcggc agagcgcaac gttcagcttg aaaaaaccct ggcggaattc aagctggccg
     9481 aacgagcctg gagtgcgtac ttggatcaag acgaaaacgc ctccgacgct tatgagtcgc
     9541 gtttttggct cgccgacgcg aggcattgga tcgtgtatac caaggtcctt gcggggcaga
     9601 gtccgaccat cggcgatgtg ctgcgcgcgc ggcagtccgc cgtggaagtc cgcgattcca
     9661 acgaggatga tcgtcgcctg gaagttgccg ccttttatgc tgtcaacctg gccttcctcg
     9721 tgcaaaacga tcggaatcgg gtctggcagg agaccagcgg cgccaaaggg ctagaggaaa
     9781 gaaataacct cgtattcgtc ggcgaggggg acaatagaaa ggtggtatcg gatcccatac
     9841 cgccagcgat tcggttccgt aatttatcgc aggaagaata tatccgcacg gtgccggaac
     9901 agcacgacat caacaaaaga tcgccgatgt tcgcgttcca ggtcgccgaa tccttcttct
     9961 tttatgggca gttcggagaa gccaaaaagc gctacgagcc catttatcaa aaagaatgcg
    10021 gaaaaaccga ctatggctac agggcctggg aacgtctgct caccatgagc aacatcgagg
    10081 gcgacgtcga gcaaagtacc aagcttgccg aggcacaaaa agcgaatagc tgtgctgtca
    10141 gtgaagagca aaaaactgcc gaaacgctgc tcatcaaccc gacgttgcag gaggctgcgt
    10201 accgcgacgc cagaaaagca ttcgatgaag cgcagaatat gcccgacggt cccgagcgca
    10261 acgaaaaatg gagaaaggcc gcggcgttat accgcaaggc cttggaaaac gcgccggatc
    10321 gaaacgaagc cccggaggcg gcgatcaacg gcgccatcgc ctacaagcaa ctcggcgaat
    10381 acgacaaagc cattgaaatg tatgagcttt tcatcaagcg ttacggcgat gaaaagaccc
    10441 tcgtggttct tgaaaagggc aaccccaaag catctccgcc cgtggctccg aaccccgaaa
    10501 aatacaagga acgtctcacc tacctcaagc aagcccataa tgcgctttcc gaagcgtata
    10561 ttctattttt cgactaccga cgggccgcgg agcaatacga tacgatcagc caaatcgaac
    10621 gtttcgacca aaaagatcgt cgggaagcag ccaagctcgc cctcgagtta tacgccaaca
    10681 tgggtgataa gggaaaaacg gccacgcttc aaaagcgctt tttgtctctt gggccgactc
    10741 cccaagagaa agctgaggcc gagtatatcg tagcgcgtgg ggaaatgaac gaatgggacg
    10801 agcgtggacg ggatgaaggg gcgaaccgcg cggcgcgaca gcgtgcgttg acggcgatga
    10861 cggccttcca cgctcgcaac aagaataacc gggatgcggc caagtatgtg gttcgcgcct
    10921 cttacaacgc cgcgaagctt caaaaagtag gtggaggaaa ccagtatcaa gactggtaca
    10981 agaaaacgat tgcggctttt gaacaatgga aagcggtagc tccaaagaaa agcgatgggc
    11041 aaagctcagc cgtttccacc gcggaagccg cgatggccgc agaagccgaa ttttctctcc
    11101 tcgatcaaga aatccgagaa aaattcgact acgacacggg ccatcaccgc tacaagggca
    11161 cgacagtcga tgtcatccgg gattatcggg cggatgctgc cgatgccaaa cgatatcacg
    11221 accgtctgca acgcgtgatc gacgtgtata tatctccaga gtgggcggtg gcggcgcgtt
    11281 ctcggcaggg atcgctgtat gactctcttc gcacgggtat gtacaacgcc cgcgagccag
    11341 cattgaagct cttctccgac aaagaagaag cgcttctgaa aaagctcgaa gccagcgacg
    11401 accccgaaca ccaggacaaa gccgacgtat tccgaaccaa tcgacaaaac gtttggcggc
    11461 aaacccggga caaggagctt accgacgcgg acaaggtcat ggtcacccgg tacgtggaag
    11521 ccttctcgtt ggggcgcaag tataacatct ccaacccagc gatagaccgt gccgtggaac
    11581 gtttggcgtt tttcaccgac gtactcggag atgccaagtt gcgagaatac tctcagggcg
    11641 tcgcagggtt cacctatacc gaccgcatgt ttttgctcag tcgtcctgga atcatcgagc
    11701 agccgcagat cgacccactc cctcatccgc ttcccgtggt gccatgatga agacccaacc
    11761 cgcaaaaacg attgcgatag cggcggtatc acttttcgtt gccgcgtgga ttcctgcctg
    11821 tggcggcaag caaggcgacg cgaaaagccc tggatcgctg agcacgtcgg agc
//
LOCUS       DAOSOR010000110        11818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5380_length_11818_cov_4.348551, whole genome shotgun sequence.
ACCESSION   DAOSOR010000110 DAOSOR010000000
VERSION     DAOSOR010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11818
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5380_length_11818_cov_4.348551"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..954)
                     /locus_tag="PK710_08115"
     CDS             complement(181..954)
                     /locus_tag="PK710_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOT09720.1"
                     /translation="MGQVLRVEVAGDTNVGRKRTHNEDAFSILGEYGLYIVADGMGGH
                     ASGEVASRMAVDTLREFFSATAEDPERTWPYKMDRSKGYEENRLITGIKLANLRIYET
                     AQRDVRKRGMGTTIVAIFTVEDGLYVAHVGDSRVYLIRDGVMEQLTEDHSLLNDYIKM
                     KRLTPEEIAKFPHKNVIVRALGMKETVKVDTQFHMPRANDTILLCSDGLSGPVSDEEM
                     QQIVTQAPDLKTATRQLIDRANENGGPDNITCVLTRWIA"
     gene            complement(1108..2226)
                     /locus_tag="PK710_08120"
     CDS             complement(1108..2226)
                     /locus_tag="PK710_08120"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HOT09721.1"
                     /translation="MNQRQTHSFLSHRAKAWMRRYTSTVSHRFHLWLCAGLLVFSFSL
                     TASSQSPAPSRPAVSETTTPASNSPKTPLATAADGVVALERHGRLLGLGFVLTGDGRI
                     VSTLSTLGDGNQIDARYADGSVVDVKIGHTDRAWNLALLVPQVGRWETGLSASTADPF
                     AEGTRVRTFAKRGRTVSVASVVLRGRTEMLGGDGELLRDAIEIATPIPATDQGAPLVD
                     EQGKVLGVVSSACKPTEGQSTAPCRPTAYGVPIGALRQFLRNAPLNAMPPSPWLGIQG
                     VGATTTIGGVRVVHVHPDSPAAAMQLQAGPPGQGNDIIVAVDGVPVHSAEMLAEQVRA
                     KAVGAEIQLIVLRGGIFHVLRTALTAPPSTSSGPKTAR"
     gene            2419..3000
                     /locus_tag="PK710_08125"
     CDS             2419..3000
                     /locus_tag="PK710_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT09722.1"
                     /translation="MGANIVANLEGQARSTHDDAAIQEALDARDYRRAVAHCARMHGA
                     AIGRLCMALVGSQAEAQELAQETLVAAYQAFPSYRAEGTVRAFLFGIARRLCARHVET
                     RARRGSILRLVHDATSVQGDAAEWLTIRQRAEQVRTALEGLRPTERDAVVLRFQGGLS
                     FREVGQSCGVDEATARKRVSRAIARLRVSLGDF"
     gene            3015..7151
                     /locus_tag="PK710_08130"
     CDS             3015..7151
                     /locus_tag="PK710_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HOT09723.1"
                     /translation="MSKQSQDDLCQRVQEQIADVLLGDVDPELLEHIADCDACRDLRF
                     EAQAVVEALEQAGADYVHPQDFETSLAARLDAIQTPADSFVSASQPPPSPVASVSDKP
                     SEPSPSSEEPSPAENLTDAPVASSAAQAPEAPKAPAPETAVVSIGQARRSRGFKMAGV
                     VVASALAVAAGFVLWSSPEKKTDSPLAESRWSATVSSVKIAGKSAENGLQRCAVDGSA
                     CEPLTDGAVVNAGSLIRTDARTRVHLEMSDGTRIAIDRATELTLGEQAGRRASLRSGT
                     IVADVMHLDDAPHAEFSLPHGNVEVLGTKFTLTASEDRSAVEVVRGSVRLSNSKGDSA
                     VVQAGEEGTIGNDSRPKVLPSATLGESLAWSERTDVEDGPEQALVRGLGELRARKPGS
                     TGELDQAVRLTKHDVKVRISGQVARTEIDETFTNDTGDVLEGIYRFPMPPDAQIERLA
                     LEVDGRLEEGAFVDKDRAAAIWRGVIQAAAPKSPKPRDEIIWVPGPWRDPALLEWQRG
                     GRFELRIYPIPAKGSRRVILAYTQSVPRSQGVRKYTYPLSYDPSGSTRIDQFDVDVQV
                     RGHDPDYGVRSRGYAFESGRADDARQLSFHARSFVPSGDMTLEYSMAEDRREVTAWTY
                     QQESDTPSSTAVAGAGDSVVPEKNGEGYVAIALRPTLPRWSEGTFRDQIIVVDSSRSM
                     VGERFKRAARLAEIVVREMDRRDRFVVLACDTQCVPMHSAMQSPGGAAAQQVRAFLDG
                     IEPDGGSDLVAMTSQAIAYAKGSEGREVRVVYLGDGTPSVGPVRPSHIMQEVSRVLGP
                     GRAAMTAVAVGADADQHALSALARGGGGVMIPYVPGENVNVVAMHVLGASYGMALRNP
                     VVQLPEGLTAVHPKVLDNIPAGTESIVVARMQHSKIKGEIKLLGKVGGEKFEQTYPIE
                     LVATTSKGNAFVPRLYAATKIQDLEANQGDGAKTELVELSKRFAVASRYSSLLVLESA
                     AMFQAFGIERNTSTMPVWTGDEAGESSGSDGVMRYDMDEPEQGFALGGGAVAESKKSS
                     YAAPPAARPAATTGVVPMDEVFESPWRDRPSRRNRGMVPMRRVWDRKATISMDTFSSV
                     ERASNEIAKMQQDVSSHPDSRTRLEKLLGLYAVTGQVDRVSDLAEQWAKRDALDPSAL
                     IARAEAAARKGQRKRAIRILGGLADVRPGDPDMQRWLASLHEAAGEKPLACSHRIALA
                     SLRSKDAVAIADAIRCANETQRGALASSLQSEADESIRAALDRELAKSPPDNKLRGDV
                     QVEALWDDDVDLDVALIGRNGQRYSWLGDPKARVTSQDATSHRRESLAVFNAPRGDYV
                     VEVTRADAVDRDVPVRGTLKIRAVGTTQTIPFVLSGTRTDVAKIRIYYSSRLVPVSGW
                     "
     gene            7231..7695
                     /locus_tag="PK710_08135"
     CDS             7231..7695
                     /locus_tag="PK710_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09724.1"
                     /translation="MTGRRRQSIQPIGASELVDAEVHGPSGAFRIFRWFESDRLTKGE
                     PADCSFSISNKFKALRRSWLEIPADGGAVWVDGAIVGGSRHAEVNTGAVGADGESPLS
                     KMTFGQRFDQVVQALAPPLVDIVLCDQDGSWALMATGAATKAGMFLQTRIMA"
     gene            7785..8156
                     /locus_tag="PK710_08140"
     CDS             7785..8156
                     /locus_tag="PK710_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09725.1"
                     /translation="MTTSDVHDQASESVKALHDESHHEPRPLEEPATPLWVTALGVGL
                     LVFGGILALVMHEPASETQEQATPSEEATESEDSEKAAEPPRERAPMRLMAPDSTGEA
                     LKRFPNLQLLRSRGQQPPGTP"
     gene            complement(8190..10325)
                     /locus_tag="PK710_08145"
     CDS             complement(8190..10325)
                     /locus_tag="PK710_08145"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOT09726.1"
                     /translation="MAVSPSPELEALLANVKKSYPDADTAPIELAFEGVTTHLDATAK
                     KRALHAANVLASLRLDPAAVGACLVATFRNQISQADTENQFGKEIAKLVDGVVRVQAT
                     RWDRLEQEAAESLRKMFIAMAAEVRVVIIVLALRVEKMRTLSNSMDESTRRRVARETL
                     EVFAPLANRLGVWQFKTELENLSLAQLEPETYSELEKLLSERRQERMRLIQQAITLLR
                     DKLPEQKIHATVQGRAKHLYSIYQKMQRKGVGLDQIHDISAVRVITEQVGDCYAALGL
                     VHSLWAPLPGQFDDYIARPKENLYRSLHTTVVGPEGKPLEVQIRTRQMHEYAEYGVAA
                     HWAYKEGRKAAKSSDQKFVVLRQLMDWERDVSDPHQFAERLKTDIFRDQVYVFTPAGD
                     IVDLPQGATPLDFAYRIHTMVGHRFRGARVNDQIVPLDYQLKTGDRVEILTHKKPNPS
                     RDWLNPASGTLRTTSARSKVRQWFREQGRDDAIQQGREIVERELHRLDLAYTTLTDVL
                     AKLEYEKLDDLYAAVGFGIRSPHSVAAAALQVERDKAPAPELPSISEPESVRRTSSTG
                     VSLSGIDDILGKRARCCSPVPGDSVLGFISRGRGVIIHRKDCPNINIAHEPERWVEID
                     WGPEGEERHAVDLDILVNDRQGLLRDLSDLVAHTGANVRSVRADTRLKKNTARIRLSL
                     DIVSSDQLLRVLDKLERHKDVVSVRRVSK"
     gene            complement(10389..11690)
                     /locus_tag="PK710_08150"
     CDS             complement(10389..11690)
                     /locus_tag="PK710_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOT09727.1"
                     /translation="MLHTTIPFGIVVGLKALRSRANVWLVMVMRRVVLWLAPLVAVVL
                     FVTDSMAQAPCATHTVGEGQSLWKIAKRYHVTVEALREQNGMVAGAPIKPGQVLEIPA
                     RKGSCKSAKGAASRPTAPTPKAASPTPEAKTETSKASNPQPVIAPPAAWTQTQKSAQE
                     RGVNPCNTPDPGFGIYDRWDRSPSMGQMISVQKGGITRSGQFDVMFHFHGHEAVRKEW
                     VQVMDGAVLVGIDLGIGSGPYESVFRSPNVFQNLVRSVEAAVAKKTGNPKAKARKIGL
                     SAWSAGYGAVGEILRTAYGKQVVDTVVLLDGLHCGYAGKSLNAVQLEPFVEFAKRSAK
                     GQKLMFVSHSSIIPPGYASTTETANYLIHALNGRAQAVRPRGSDPMGLDLISRYDRAG
                     FHVRGYQGNDKMDHCAHIGLLRDILRVHVKRRWQSPKGRKA"
BASE COUNT         2395 a         3189 c         3668 g         2566 t
ORIGIN      
        1 gggacgcggt gcgctgcatg tgctcgtgca agtcgaagtg cccaaaaaag tcagtgcgaa
       61 ggcaaaatct ctgttgcgcg aactcgaaaa ggaactcgct cagtcttcca ccgacaaaca
      121 ggccatcggc agctaatcac gaactccacc aggcgaataa aaaaccgttg gcttgttcgt
      181 tcaggcaatc caacgggtca acacacacgt gatgttgtcg ggaccaccgt tttcattggc
      241 tcgatcgatg agttgacgag ttgccgtttt caagtccggt gcttgcgtca cgatctgttg
      301 catctcttcg tcgctcacag gaccactcag accatcggag cacaacaaga tggtgtcatt
      361 ggctcgtggc atatgaaatt gcgtgtccac cttgacggtt tctttcatgc caagggcgcg
      421 gacaatgacg tttttatgcg gaaatttggc gatctcctcg ggcgtcaagc gcttcatttt
      481 gatgtagtcg ttgagcaagg aatggtcctc ggtcaactgc tccatcacgc catcacggat
      541 gagatacacg cgactgtctc cgacatgggc cacgtacaat ccgtcttcga ccgtaaaaat
      601 ggccacgatg gtggtaccca tgcctcgttt gcggacgtcg cgttgggcgg tttcataaat
      661 gcgaaggttg gcaagcttga ttcctgtgat gagccgattt tcttcatacc ctttgctgcg
      721 gtccattttg tagggccacg ttcgctccgg gtcttccgcc gtggcggaga agaactcacg
      781 cagggtatcg acagccattc tggacgccac ctcccccgag gcgtgcccac ccattccgtc
      841 ggcaacgata tataggccgt actcaccaag gatggagaac gcgtcctcgt tatgcgtgcg
      901 tttacggccg acattggtgt cgccggcgac ttctacgcgg agtacttgac ccacaaccaa
      961 cttatacctt gaaccgtagg gctcttgcga gttttaagga gcaaacaacg ctcgaagctt
     1021 accttgaccg aacaactatc catcgagatg gcggtcggcg tcaactcttt gccaggatgc
     1081 gaatcagaga gcaaggggca caagaggcta ccgcgcggtt ttcgggccgg aagacgtgga
     1141 aggtggggca gttagggcgg ttcgaaggac gtgaaaaatt cctccacgca gcacgatgag
     1201 ctggatttct gcgcctactg ccttggcgcg cacctgttcg gccagcattt ccgcggaatg
     1261 cacgggcacc ccatccacgg ccacaatgat gtcatttccc tggcccggcg gaccggcttg
     1321 taactgcatg gccgcggcgg gactgtcggg atggacatgg acgacgcgca cgccgcctat
     1381 ggtggttgta gctcccacac cttgaatgcc aagccaaggg ctcggtggca tcgcgttcaa
     1441 aggggcattg cgcaaaaact gcctgagcgc ccctatcgga accccataag cggtcggacg
     1501 gcacggagcg gttgattggc cttcggtggg tttgcaggca ctgcttacta cgccgagcac
     1561 ttttccctgt tcatcgacca agggcgctcc ttggtccgtg gctggtatcg gtgtggcgat
     1621 ctcgatcgca tcgcgcaata gctcaccatc gccacccagc atttcagtgc gaccacgcag
     1681 caccacggaa gcaaccgata cggtgcgtcc ccgtttggca aaggtccgaa ctcgcgttcc
     1741 ttccgcaaaa ggatcggctg tcgatgccga cagccccgtt tcccaacggc ccacttgagg
     1801 caccaagagc gccagattcc aggctcgatc cgtgtgtcca atcttgacgt cgaccacact
     1861 tccatccgcg tagcgtgcat cgatctggtt gccatccccc aacgtggaca gtgtcgagac
     1921 gattctgcca tcgcccgtaa gcacgaaacc caggccaagc aatcgtccat ggcgttctag
     1981 cgcaacgaca ccatcggcgg ctgttgccag gggtgtcttg ggtgagttcg acgcgggtgt
     2041 cgttgtttcc gaaacggcag ggcgagatgg cgctggggat tgactcgatg ccgtcagtga
     2101 aaacgaaaaa acgaggagtc cagcacataa ccagaggtgg aaacgatggc tcacggtcga
     2161 agtgtagcgc cgcatccacg ctttggctcg atggctgagg aaagaatgcg tttgtcgttg
     2221 attcatggtg gtattgtcga agttgggttg ctttgggaaa tcccatgcaa ggacgattcc
     2281 caccggttga ggtgtagggc tgatagggcg acgtgcgttg tcgattgggc gacatgggat
     2341 tgcaaaaaaa atattcggca catcggtcac atttcgaggc gtggcgggca catggaacca
     2401 acacaggacg aaaaagccat gggagcaaac atagtggcga acctcgaagg gcaggcgaga
     2461 tcgacccatg acgatgcggc catccaagag gccctcgatg ccagagacta ccgaagggcc
     2521 gtagcgcatt gtgctcgcat gcacggcgcc gccatcgggc gcttgtgtat ggcccttgtc
     2581 ggctcccagg cagaagcgca agaactcgcg caagaaacgt tggtcgcggc ctatcaggcc
     2641 tttccttcct atcgtgccga agggacggtt cgggcgtttt tgtttggcat tgcacgacgc
     2701 ttatgtgcgc gtcatgtcga aacacgcgcc cggcgaggtt ctatcctccg tctcgttcac
     2761 gatgccacat cggttcaagg ggatgccgcg gaatggctaa cgattcgtca gcgcgcggaa
     2821 caggtgcgta ctgccttgga agggctgcgt ccgacggagc gggatgcggt cgttcttcga
     2881 tttcagggtg ggctgagctt tcgggaagtg ggccagtcct gtggtgtgga tgaagccacg
     2941 gcgcgaaagc gcgtgagccg tgccatcgcg cggttgcgtg tgtctttggg cgatttttga
     3001 aggagcagtg ggccatgtcg aaacaatccc aagacgatct atgccagcgg gtgcaagagc
     3061 agatagccga tgttttgctg ggagacgtag accccgaact ccttgaacac atcgctgatt
     3121 gtgatgcgtg tcgagatctg cgattcgagg ctcaggcggt cgtggaagcc ttggagcaag
     3181 caggggcgga ttacgttcat ccgcaagact tcgaaacgtc gcttgcggcg cgcctggatg
     3241 ccatccagac ccctgccgat tcgttcgttt ccgcttccca gcccccgcca agcccggtgg
     3301 catctgtttc cgataaacct tccgagcctt ccccatcgtc cgaagaacct tctcccgccg
     3361 aaaatctcac cgacgctcct gttgcgtctt ccgcagccca agctcctgag gctccgaagg
     3421 caccagcccc ggaaactgcg gtcgtttcca tcggccaagc acggcgaagc agggggttca
     3481 agatggctgg tgtcgtggtg gcgtcggcac tggccgtggc ggcaggcttt gtcctctggt
     3541 catctcccga gaaaaaaacc gactctcccc ttgccgaaag ccgatggtcg gcgacggtct
     3601 cctcggtaaa aattgcggga aaaagcgcgg aaaatggctt gcagcgatgt gccgtcgatg
     3661 gttcggcctg tgaacctctc accgatggag ctgtcgtcaa tgccggctct ttgattcgaa
     3721 ccgatgcaag aacccgcgtt catctcgaga tgtccgatgg aacgcgaatt gccatcgatc
     3781 gtgcgacgga attgacgcta ggcgagcaag ccggtcgcag ggcctctttg cgttccggca
     3841 cgatcgttgc cgatgtcatg catctcgacg atgcccccca tgccgagttt tcactacctc
     3901 atggcaacgt ggaggtgctc ggcaccaagt tcacgttgac cgcgtccgaa gaccgatccg
     3961 ccgtcgaagt cgtgcgaggt tcggttcgtc tttcgaattc gaaaggcgat tcggccgtgg
     4021 tgcaggccgg agaagaaggc acgatcggta acgactcgcg tcccaaggtt ttgccgtcgg
     4081 ccacgttggg agaatcgctg gcgtggagtg aacgaacgga cgtggaggat ggccccgagc
     4141 aggcgttggt tcgaggtctt ggagagcttc gagcgcgcaa gccgggctcc acgggagagt
     4201 tggatcaagc ggtgcggttg accaagcacg atgtgaaagt tcggatatcc ggtcaggtcg
     4261 cgcgtaccga gatcgatgaa acgtttacga acgatacggg cgatgtgctg gagggaatct
     4321 ataggtttcc catgcctccg gacgcgcaga tcgagcgtct cgccctcgag gtcgacggcc
     4381 gtctcgaaga aggggcgttc gtggataaag accgggccgc ggccatttgg cgaggtgtga
     4441 ttcaggccgc cgctcccaaa tctcccaaac cgcgtgacga aatcatttgg gtgccgggcc
     4501 cttggcgcga ccccgctttg ctcgaatggc agcggggggg acgattcgaa ttacggattt
     4561 atcccatccc cgcgaaaggt tcccgtcgcg tcattctggc ctatacccaa tcggtccctc
     4621 gttcccaggg tgtgcgtaag tatacgtatc cattgagtta tgatcccagt ggctcgaccc
     4681 gtatcgacca attcgacgtc gatgtgcaag tgcgtggtca cgacccggac tacggtgtgc
     4741 gctcgcgagg ctatgccttc gaaagtgggc gagccgacga cgcacggcag ctctcgtttc
     4801 acgcccgatc gttcgtcccc tcgggagaca tgacgttgga gtattcgatg gcggaagatc
     4861 ggcgtgaggt gacggcttgg acgtaccaac aagaaagcga taccccatcg tccacggctg
     4921 tcgctggtgc tggtgattct gttgtccccg agaaaaatgg cgaagggtat gtggcgatcg
     4981 cgttgcgtcc cacgcttcct cgatggtccg agggcacgtt ccgtgaccag atcatcgtgg
     5041 tcgattccag tcgttcgatg gttggggagc gtttcaaacg agctgcgcgg cttgcggaaa
     5101 tcgtggtgcg cgaaatggac cgtcgtgatc ggttcgtcgt tcttgcgtgc gatacccaat
     5161 gtgtaccgat gcattctgcg atgcagagtc ccgggggggc tgcggctcag caagtgcgag
     5221 cgtttttgga tggaatcgag ccggatggcg gcagtgattt ggtggcaatg acaagccagg
     5281 cgatcgcgta tgcgaaggga agcgaagggc gagaggttcg ggtggtgtac ttgggcgatg
     5341 gaacgccgag cgtggggccc gtgcgtcctt cacacatcat gcaagaggta tctcgcgtat
     5401 tggggcctgg tcgggcagcg atgacggcgg tggcggtggg cgcggatgcg gatcaacatg
     5461 cgttgtcggc gctggctcgt gggggagggg gcgtgatgat tccctatgtt cctggcgaga
     5521 atgtgaatgt ggtggcgatg catgtccttg gggcgagcta tgggatggcg cttcgaaatc
     5581 cggttgtgca acttcccgaa gggctcaccg cagtgcatcc gaaagtgttg gacaacatcc
     5641 ccgcgggcac cgagagcatc gtggtggcac gaatgcaaca ctcgaaaata aaaggagaaa
     5701 ttaagcttct gggcaaggtg gggggagaga aattcgagca gacctatccg atcgaattgg
     5761 tggcgacgac gagcaaaggc aacgcttttg tgcctcggct ctatgcggcg acgaagatac
     5821 aggatttgga agcgaatcaa ggggatgggg ccaagaccga acttgtggag ttgtccaaga
     5881 gattcgcggt ggcgagccgt tattcgtcgc ttttggttct tgaaagcgcg gcgatgttcc
     5941 aggcgtttgg catcgaaagg aatacgtcga ccatgccggt atggacaggt gatgaagcgg
     6001 gcgagagcag tggctccgat ggggtgatgc gatatgacat ggacgaaccg gagcaggggt
     6061 tcgcgctggg gggaggcgcg gtcgcggaat cgaagaagag ttcgtatgcg gcccctccgg
     6121 cagccaggcc agccgccacg acaggggtcg tccccatgga cgaggtgttc gagtcaccat
     6181 ggcgagatag accgtcgcgg cggaatcgag gcatggtgcc catgcgacgc gtttgggatc
     6241 gaaaagctac catttcgatg gatacgtttt cgagcgtgga acgggcttcg aatgagattg
     6301 cgaagatgca gcaggatgta tcgagccacc cggacagccg cacccgtttg gaaaagcttc
     6361 tcgggttata cgcggtcacg ggacaagtgg atcgagtctc ggacttggcc gagcaatggg
     6421 ccaagcgaga tgctctcgac ccgagtgccc tgatagcgcg tgcggaagcc gcggctcgaa
     6481 aaggccaacg aaagcgggcg attcgtattc tggggggtct tgcggacgtg cgtcctggtg
     6541 acccggatat gcaacgatgg ctggcgtcgc tgcacgaagc ggcgggagaa aaaccgctcg
     6601 cctgttcgca tcgcattgcg ttggccagcc tgaggtcgaa agacgcggtg gccatcgcgg
     6661 atgccattcg ttgcgcgaat gaaacccagc gaggcgcgct tgcgtcgagc cttcaatccg
     6721 aagcggacga gtcgattcgc gcggcgctgg atcgcgaact cgcgaaatct ccaccagata
     6781 ataagctgcg aggcgatgtt caggtggagg ctctgtggga tgatgacgtg gacctggatg
     6841 tggcgttgat tgggcgtaat ggacaacgat actcgtggct cggcgatccc aaggctcggg
     6901 taacgtcgca agacgctaca tcccatcgtc gcgaatctct cgcggtgttc aatgccccga
     6961 gaggtgatta tgtggtggaa gttacacggg cagatgcggt tgatcgcgat gttcccgtgc
     7021 gcggtacgct caagattcgt gcggtcggaa cgacacaaac cattccgttc gtcctgtcgg
     7081 gaacgagaac ggacgtggcg aaaatccgca tctattattc ctcacggctc gtacccgtgt
     7141 ccgggtggta aaccttgccg agccctgttc gaaggaccca gcatttacac agcaacccaa
     7201 ggctttcccg tcgatgatgc ctcaacgccg atgacgggtc gtcgtaggca aagcatccaa
     7261 ccaataggag cgtcggagtt ggtcgatgcc gaagtgcatg gcccatccgg ggcctttcgt
     7321 atctttcggt ggttcgagtc ggatcgattg acgaaaggcg agccagcgga ttgctctttc
     7381 tctatctcta ataaattcaa ggcattgcgg cgatcctggc tggaaatccc ggcagacggc
     7441 ggcgcggtct gggtggatgg agcaatcgtt gggggctccc ggcacgccga ggtaaacaca
     7501 ggcgccgttg gggccgatgg ggaaagccca ttgtccaaaa tgaccttcgg tcagcgattc
     7561 gatcaagtgg tgcaggccct tgcgccgcca ttggtcgata tcgtcttgtg tgaccaggat
     7621 ggttcctggg cgctgatggc aacaggcgcc gcaacgaagg cagggatgtt cttgcaaact
     7681 cgcatcatgg cataaccggg tacgaaacgc aggggggatt gctcgatggg aagggtgaat
     7741 ggcggttgac gtcgggctga cggttgaggc atctgtggga tgcgatgaca acgagcgatg
     7801 ttcacgatca ggcttcggag tcggtgaagg ctttgcatga cgaaagtcat catgaaccaa
     7861 ggccgctgga agaaccggcc actccgctgt gggttacggc gctgggggtg ggtttgctgg
     7921 tttttggggg aatattggct cttgtgatgc atgagccggc gagcgaaacg caggagcaag
     7981 cgacgccctc ggaggaggcg accgagtcgg aggattcgga aaaagcggcg gaaccgcctc
     8041 gcgagcgggc gccgatgcgt ttgatggctc cggattccac gggtgaggct ctcaaacgat
     8101 ttcccaatct tcaattgctt cgttccaggg gacaacagcc gccagggact ccgtgaagca
     8161 cgagactcga tgctggacgg gcgcgggtat tacttgctga cccggcgaac ggatacgacg
     8221 tctttgtgtc gttcgagttt gtcgagcacg cgcaggagtt gatcggagct gacgatatcg
     8281 agcgataggc ggattcgggc ggtatttttt ttgagcctgg tatcggcacg aacggaacgc
     8341 acgttggccc ccgtatgggc gaccaggtcg gaaaggtcgc gtagcaggcc ttgccggtca
     8401 ttcacgagga tgtcgagatc gacggcatga cgttcttccc cttcgggtcc ccaatcgatt
     8461 tcgacccatc tttctggttc gtgggcgatg ttgatattgg ggcagtcttt gcgatgaatg
     8521 atgacgccgc gtccacgact gatgaatcca aggaccgaat ctccgggcac ggggctacaa
     8581 catcgcgctc tttttccgag gatatcgtcg attccggaaa gagatacgcc cgtcgaggaa
     8641 gtgcgccgaa cgctttccgg ttcgctgatc gaaggtagtt cgggagccgg ggccttgtcg
     8701 cgttcgacct gcagggcagc ggcggccacg gaatgagggg agcggattcc gaagccgacg
     8761 gcggcatata ggtcatcgag tttttcgtat tcgagtttgg cgagaacatc ggtgagcgtg
     8821 gtgtaggcca ggtcgagccg atggagttct cgttcgacga tttcgcgtcc ctgctgaatg
     8881 gcatcgtcgc gtccttgttc gcggaaccac tggcggactt tgcttctggc actcgtggtg
     8941 cgcagggtac cggaggcggg attgagccaa tcgcggctgg ggttgggttt tttatgggtg
     9001 aggatttcga cccggtcccc ggttttgagt tggtaatcga gcgggacgat ctggtcattg
     9061 acgcgtgcgc cacggaatcg gtgtccgacc atggtgtgga ttcggtacgc gaagtccagg
     9121 ggcgtggcgc cttggggtaa gtccacgatg tcgcctgccg gggtgaaaac atacacttga
     9181 tcgcgaaaaa tgtcggtttt gagccgctca gcgaattgat ggggatccga aacgtcgcgt
     9241 tcccaatcca tcagttgccg aagcaccacg aacttctggt cggacgactt ggctgccttt
     9301 cgtccttctt tatatgccca atgcgcggcc acaccgtatt cggcatattc atgcatctga
     9361 cgggtacgaa tctgcacttc cagcggtttg ccttccggtc ccaccacggt cgtatgaagc
     9421 gatcgatata ggttctcttt cggtctcgcg atgtagtcgt cgaattgccc cggcaacgga
     9481 gcccatagac tatgcacgag accgagcgcg gcgtagcaat cgcccacctg ttcggtgatg
     9541 acgcgcacgg cgctgatgtc atggatctgg tcgagtccca cgcccttgcg ctgcattttt
     9601 tggtatatgg aatacaagtg cttggcgcgt ccctggaccg tggcatgaat tttttgttcg
     9661 ggtagtttgt ctcgcaacaa ggtgatcgct tgttggatga gacgcatgcg ctcctgtctt
     9721 cgttccgaca gaagcttttc gagttcggag taggtttcgg gttcgagttg cgccagagac
     9781 aagttctcca actcggtttt gaactgccaa acgccaaggc gattcgccaa gggagcgaat
     9841 acttcgaggg tttcccgagc cacgcgacgc cgggtggatt catccatcga gttgctcaag
     9901 gtgcgcattt tttctacacg taacgccagt acgatgatca ccacgcgcac ttccgccgcc
     9961 atggcgatga acattttgcg taatgattcg gcagcttcct gttccagacg gtcccatcgg
    10021 gttgcctgga cccggaccac accatcgacc agctttgcaa tctcctttcc gaactgattt
    10081 tccgtatctg cctgggagat ttgatttcga aacgtagcga cgagacaagc gcctacggcc
    10141 gcggggtcca gtcgcaggct cgccagtacg ttggcggcgt gcaaggctct tttttttgcg
    10201 gtagcgtcga gatgggtggt gacgccttcg aaggcgagtt caatgggtgc tgtatccgca
    10261 tccggatagc ttttttttac gttggcaagc agtgcctcca actcgggcga tgggctaacg
    10321 gccatcttag ggaaactatc acagccagtg cttcgtcccc accgcgaatg ctttttgtcc
    10381 caagcttttt atgctttacg ccctttggga gattgccatc gtcgcttgac gtgtaccctg
    10441 aggatatcgc gtagcaaccc gatatgggcg caatggtcca tcttgtcatt tccctggtat
    10501 ccacgcacat ggaagccggc gcggtcataa cgagaaatga gatcgagacc catcggatcc
    10561 gaaccacggg gccggacagc ttgggcacga ccgttgagcg catgaatcaa ataattggcg
    10621 gtctccgtcg tagaggcata tcccggagga atgatcgagg aatgactcac gaacatcagc
    10681 ttttggcctt tggccgaccg cttcgcgaat tcaacgaatg gttcgagttg caccgcgttg
    10741 agcgatttgc ccgcgtaacc acaatgaagg ccgtcgagca ataccaccgt gtccaccact
    10801 tgtttgccat aagctgttcg taaaatttct cctaccgcac cgtatccagc actccacgca
    10861 ctgagtccga tcttgcgggc ctttgctttg ggattgccgg tttttttggc taccgccgcc
    10921 tcgacgcttc gcacgagatt ttggaatacg tttggggagc gaaacacgga ttcataaggt
    10981 cccgatccga ttccgagatc gattcccacc agcaccgctc cgtccataac ctgcacccat
    11041 tccttgcgaa ccgcctcgtg gccgtgaaag tggaacatta cgtcgaattg gccggaacgg
    11101 gtgattcctc ccttttgcac actgatcatt tgccccatcg aaggagagcg gtcccatcga
    11161 tcgtagatgc cgaatccagg gtccggcgtg ttgcaagggt tgactccacg ctcctgcgcg
    11221 cttttttgcg tttgggtcca cgccgctgga ggagcaatga ccggctgtgg gttcgatgcc
    11281 ttgcttgtct ccgtcttggc ttccggcgtt ggcgaagccg ctttcggcgt aggagcggtg
    11341 ggacgggatg ccgcgccttt cgcgctttta cacgagcctt ttcgagcagg gatttccaag
    11401 acctgccctg gcttgatcgg tgcccccgcc accatcccgt tctgctcgcg aagcgcttcc
    11461 accgtaacgt gataccgctt ggcgatcttc cacaacgact gcccttcccc taccgtatgc
    11521 gtcgcacacg gggcttgcgc catggaatcc gtcacaaata atacaaccgc caccaaaggc
    11581 gccagccaca acacgacgcg tcgcatcacc atcacaagcc aaacgttggc gcgggatcga
    11641 agggctttca acccaacgac gataccaaag gggattgttg tatgtaacat tttgaattta
    11701 catcaaaata atgatatcag cgctggagcc aggtgagggc ggcctggtac atcgcacgac
    11761 gtttttccag cgcgacttca tgtcccatat ccgcaggatc cgagagaaaa acaggaac
//
LOCUS       DAOSOR010000111        11802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5394_length_11802_cov_4.564570, whole genome shotgun sequence.
ACCESSION   DAOSOR010000111 DAOSOR010000000
VERSION     DAOSOR010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11802
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5394_length_11802_cov_4.564570"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1100)
                     /locus_tag="PK710_08155"
     CDS             complement(135..1100)
                     /locus_tag="PK710_08155"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT09728.1"
                     /translation="MSDPNKKSPRTFQCRDVLWDTFEQMARELECSIDYLINEAMKQY
                     ARQRSYGSRTPFPGRSDAQAPPGPPGPVAPPPGAPPAAPPPMPMPATAGPPGPPPLPG
                     GPPPPPKRASLPPPPPPRARPAGPAPGAVPSPPAASPPLPVAPGSGRFPPPPPPKRGG
                     PPGYPPPPARSAPPPMPPQGGYPPPPPPGPPMAPPPGPPGPPPFPGRQPAPPPQPQAF
                     QPAPAAGPGYGPQLTVWYNNQAYPISKDRFIIGRGKQSSDLTIKDPNVSRQHAMIEFL
                     NGQYYMVDMGSTNGVEYNGQRIARKVVNDNDTFRICDHDLRFSFH"
     gene            complement(1160..2821)
                     /locus_tag="PK710_08160"
     CDS             complement(1160..2821)
                     /locus_tag="PK710_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09729.1"
                     /translation="MRTNRLSCWGLTALAIAMMTAASSSGCRVDQDDIQRWEKTERGP
                     DKLVAVIAHDKYDPTLRVEAALALVRMKPRGGRRIGIPIMVDAIAQLTDSEIRKTIIA
                     GMVPALVSEIGKPVPVAQANQPAPPDTSIPFKDAAFALLIRENSPGGKIVSEEQHRQA
                     LMQALASWVTVDFEHRYDNASQMYGVEQIIKYIGAPAARMLPSLIHNDQRKISDLARI
                     IAANGDKPTKEEASKRVVAVAAHTISAKWLDSVKSTVEEANKANKLKPTEAQFAAQLT
                     ALQDEQLKRLFGSMRQLGGRAAVDFCFDFALNKENSAERRALAMAALEGNFDQQNPND
                     VSRILSLAAADDTPDQVRDLAFRRVGEMRREKVIEKLYEIFKSERWQARWVAAQYAIG
                     MSNTSNIPEILNHLPRGGSPVFAMTEALSYGDWMGNPERMRVQGKDARAQLEPYFRDS
                     NSAVRTSALGWFFGHGTKHDIAALEPFFQDKGSVPKCGSNQSDCDWTCYVDKEGGKPD
                     EKEPKEATNIGEFIRFCIVPTIKDRNEDPSKKKDANQPATVTEKK"
     gene            3042..3734
                     /locus_tag="PK710_08165"
     CDS             3042..3734
                     /locus_tag="PK710_08165"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOT09730.1"
                     /translation="MTAPRSETPRLEFADDLPRQESRAFRYGREAASLVLIAGAVFLT
                     LALASFSHDPAVSEVTGTNWVGPVGEALANVFVSTLGLAAWIIPVELVLISLPLFRNK
                     PTMASVARIGGDTVVILIVASLIHVSFPQSTAFGSMSVGGTIGELFGELMRSLFSNVG
                     SYLIGLTAIGLVHRSRILFFHLFRPTRWTNHRTRRDEKRRGVTRHQTRVASRSNHRKS
                     PRRTRSHSYHHR"
     gene            3628..5667
                     /locus_tag="PK710_08170"
     CDS             3628..5667
                     /locus_tag="PK710_08170"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOT09731.1"
                     /translation="MKSAEGLHAIKHAWHRARTIEKVRAEHGAIRITTADRDDAIVAI
                     DTEVEAARERALAKATEMIEALHQEPLSPLEDIDEGIVLESPLHLQPVSSPTSPEPTI
                     EDDLEQDARAHESAPVIDLSASMTPMESKAATLTQPAAEETPRRKQRQAKGPTIVDTT
                     SATEVEKVKQTAATSTVPGYQMPPFDLFDEGEAPALIIDETLQQQLNDNAAQLITTLN
                     HYGVRGEIREIQPGPTVTTYEMEPEAGTKVSKIASLADDLAMSLGCQVRIIAPIPGKK
                     RVGFEIPNRERKAVNLRELIEDRRFQEMKAPLPVVLGRDIVGKPFYADLSSMPHVLVA
                     GATGAGKSVGLNVMLVSLLCRRSPDDLRLLMVDPKVVELQSFDRIPHMLLPVVTDTRQ
                     AANALKWAVDEMERRYQLLANAGTKNITTYNAWVKRVHQGEVRGFELPKPTVVTSEDG
                     SVVEFGAEKEPPKLPEKLPYIVIVVDEFADLMMQQGKEVEASIARLAQKARAAGMHVI
                     LATQRPSVDVITGMIKANFPSRIAFKVTQRNDSRTILDEQGAEHLLGRGDMLVKLNGS
                     NETRRVQCPFVSEEEVERVTSFLRQQGEPTYDDSILLPRGDETEPEEDAEQDPKYDEA
                     VMVVAETRRCSTSWLQRKLGIGYNRAAKIVEMMEKRGLVGPAQGAKDREVLIESP"
     gene            5664..5987
                     /locus_tag="PK710_08175"
     CDS             5664..5987
                     /locus_tag="PK710_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09732.1"
                     /translation="MKHATCQRILGIDLVDRSDKTPAAVLNGSKNQDLPPHGEPGLDE
                     QERTELISTIVTVIRRRGVPPHVHQAALTLMGWLARRKICEHPCQTGVAEAHQQSLRM
                     TSSRR"
     gene            complement(5993..7252)
                     /locus_tag="PK710_08180"
     CDS             complement(5993..7252)
                     /locus_tag="PK710_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOT09733.1"
                     /translation="MTKLNRQPIVVASVVALVFLSLAVLKHRAATRPIPQARQALPVG
                     SWIVAPAISSTPNRPLDPKALVVAGPPTMFHLDPAHRNRSPFVGPASPSLSVLADLHA
                     PIQTAPAVLPNGTIVVGTLDGHLFGLREDGTVVFKTPLEDRLYASPLVWKDRLFIGTD
                     DDRFVSLSSTGNVLWSLATDGDADTSASPTPDHGLVFAANETLFFARMDGTVVWRVRA
                     KRKIYSSPAVDSDGSVYVGAQDQRVYGIRRTGVVRFATRLEQQVDCAPSIGEYGLLYV
                     GTDGGAVVALDTQTGQVRWKTTVQGHVRGGLTVTRSHDVVAGVYGPSPRVVAMRGDTG
                     EQLWSFSIPGTGAKEYGIHGSPVEDAQGNLYFGAQDDAVYSLTARGAFRWKAMTAGDV
                     DAPVVIVSDGVLLAGSDDGKLYRITEQ"
     gene            complement(7274..7927)
                     /locus_tag="PK710_08185"
     CDS             complement(7274..7927)
                     /locus_tag="PK710_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09734.1"
                     /translation="MLKRGRPVLLWTVGLVMMASACAPPQVRLEPGPREYVAADYQAV
                     LQKWTRSDQLIALSELDDLLTVTATFESYDFRWAYVVRYAQDYRLTVDQRRRLLERTL
                     ADAQERHQFFVALYGTNWRWVDLNKPDSGWIVRLIDDVGNETAPEEIVTIRKPGPIER
                     TYFPYTSPWRRVYRIRFPIVTAEGHPTISNQARWFGLRFAGAQGNQELRWELEQSTR"
     gene            complement(8021..8962)
                     /locus_tag="PK710_08190"
     CDS             complement(8021..8962)
                     /locus_tag="PK710_08190"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOT09735.1"
                     /translation="MSPMRATSGRAVSATILQRLQHALAQGRPLTYLELAQQAGCTVR
                     SVRNYLQHAEQLLGFDVEKKRDRGRVLVRAVLSSPSSLPPSSCFRSDASPLDSALSDR
                     LFPPSTVNPLSCVQIAFPNLPHYAEHHARVCQAWIRHAQSRTHALRFLIPTVTSTAPL
                     WPLGIVVHHHMGIVLIGFPCHETDPKNRWTLELIRLPNESTLWEPIEREAPDWVKALS
                     VRDLFDSPFCFLSDQEDKTIDVHVRFDASLTERLRHCVWHSSQSVVLRTDGMLDVRFG
                     PVSLAWAASWVSSFGASVTVLGDKKLRKTVKKRSFSV"
     gene            complement(9067..9462)
                     /gene="rpsI"
                     /locus_tag="PK710_08195"
     CDS             complement(9067..9462)
                     /gene="rpsI"
                     /locus_tag="PK710_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOT09736.1"
                     /translation="MPTEHRFYATGKRKSAVARVWISPGSGQFIVNNTLADDYFERET
                     TRMICRQSLELLEALDEIDVWATVKGGGHSAQAEALRHGIARALIEQDPERRGMLKRA
                     GYLTRDAREKERKKYGQPGARKRFQYSKR"
     gene            complement(9468..9905)
                     /gene="rplM"
                     /locus_tag="PK710_08200"
     CDS             complement(9468..9905)
                     /gene="rplM"
                     /locus_tag="PK710_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOT09737.1"
                     /translation="MNRTFSAKPAEAAASREWWVVDAEGKSLGRLASEIAKILRGKHK
                     PTFTPHVDTGDFVIVINAAKVGLTGSKLDKKHYYRHSGSPGGFRAEPYRLLMNRKPEL
                     PIQKAVKNMLPKNVLGRQMIGKLKVYATADHPHAAQKPQPLEI"
     gene            complement(10079..10540)
                     /locus_tag="PK710_08205"
     CDS             complement(10079..10540)
                     /locus_tag="PK710_08205"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HOT09738.1"
                     /translation="MKRSDPNGGVCLCMGVTKSMDHQNIQTVRVVAAVVENNGRYLIT
                     QRRPTAVLPLMWEFPGGRVEPGETDTAALQREVRHRLGVDISVGQLISFVSHPYERYV
                     VDLYLYECRLTGGEPSPRNVHAFRWASSAEFDQYPFTPADEASMSQLLGMP"
     gene            complement(10574..11680)
                     /locus_tag="PK710_08210"
     CDS             complement(10574..11680)
                     /locus_tag="PK710_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT09739.1"
                     /translation="MITCSKCGKENQDHYKFCLGCGAELPRDAAPKPFAPRTPPQGMK
                     AVSGGKSSPAVAAYHAPAPAPSPAPFVPPAVAPRPVAEPSPAPFVPPAVSPAPVAEPA
                     PAPIVADAPMASATGETVACPQCSHVNSTAFKFCASCGYNLTKVVSVPAAPAVTSASA
                     ATWKLTVLRADGTDAGTFDVPSDVTLVGRSTGGIFGGDSYLSPQHAKLSLRGGKLFVE
                     DAGSLNGTYLKLMRDTPESVADGQYFRIGQEIIRFEKLHPEPPIDGVEKMGSPSTGYI
                     GRIVLVTGRQMDGNAFPVPKLGVHIGRERGHVLFPEDGYVSGLHCSLTVHDEEVLLTD
                     LGSSNGSFIRLMSEREVQDGDILLMGQQLFRVGH"
BASE COUNT         2580 a         3288 c         3491 g         2443 t
ORIGIN      
        1 tgtgtttcga agcgttatcc acgagcacgg gattgggatc tcggtgaagt ggtattggag
       61 gcgggatttg ggagaagggc tccgcggaac aagcgtaacc tattgaaata acaggtattt
      121 aagatgtccc gggattagtg gaaggagaag cgaaggtcgt ggtcgcagat acggaaggta
      181 tcgttatcgt tgacgacttt tcgagcgatg cgctgaccgt tgtattccac accgttggtg
      241 gagcccatat cgaccatata gtactggccg ttgaggaatt cgatcatggc atgttggcgg
      301 gagacgttgg ggtctttgat ggtaaggtcg gaggattgtt ttccacgacc gatgatgaac
      361 cggtctttgg agatggggta agcttggttg ttgtaccaaa cggtgagttg cgggccgtag
      421 ccgggacctg cggcgggggc aggttggaag gcctggggtt gtggaggggg cgcgggttgg
      481 cgtcctggga agggaggcgg acctggcggg ccaggagggg gtgccatggg cggtcctggg
      541 ggaggcgggg ggggatatcc accctggggc ggcatgggtg ggggagcgga gcgcgccggg
      601 gggggaggat agcctggagg tccaccgcgt tttggaggcg gtgggggagg gaatcggccc
      661 gagcctggag caacgggaag aggggggcta gccgcggggg ggcttggcac ggcacctggg
      721 gcaggaccgg cgggcctggc ccggggcggt gggggaggcg gcaagcttgc gcgttttgga
      781 ggcggcggag gtccaccggg caaaggtggg gggccgggcg gtccagcggt agctggcatg
      841 ggcatggggg gaggtgcggc aggaggcgcg cctggaggag gcgcgacagg tcctggaggt
      901 cctgggggag cttgggcatc actacgtccc ggaaacgggg ttcgtgagcc atagctgcgc
      961 tgccgcgcgt attgcttcat cgcctcgttg atgaggtaat cgatggagca ctcgagttcg
     1021 cgcgccattt gctcaaaggt atcccaaagg acgtctctac actgaaatgt gcgggggctt
     1081 ttcttgttcg ggtcactcat ggatgcgcct tgatcgtact gccctcacgc cttcgcgtga
     1141 aacttttgct tcttcgactt cacttctttt ccgtcacggt tgccggttga ttggcgtcct
     1201 tctttttcga agggtcttca tttcggtctt tgatggtagg aactatgcag aaccgaatga
     1261 attctccgat atttgtagcc tctttgggtt ctttttcatc gggttttccc ccttctttgt
     1321 cgacatagca ggtccaatcg cagtcggatt ggttggagcc acatttggga accgaccctt
     1381 tatcttgaaa aaacggttcg agcgcggcga tgtcgtgttt ggtgccgtgt ccgaaaaacc
     1441 atcccagggc gctggttcgc acagcactat tggagtcacg aaaatagggt tcgagttgcg
     1501 cgcgggcatc tttgccttgc acgcgcattc gctctgggtt gcccatccaa tcgccgtagc
     1561 taagagcttc cgtcatggcg aacacaggag aaccgcctcg gggaagatga ttgaggatct
     1621 cggggatgtt cgaggtattg gacataccga tcgcatattg agccgctacc caacgggctt
     1681 gccaacgttc gcttttgaaa atctcataaa gcttctcgat gactttttct cgtcgcatct
     1741 ctcccactcg acgaaacgcc aagtcgcgca cctggtcggg cgtgtcatcg gccgccgcga
     1801 gggaaaggat gcgagagaca tcattgggat tttgctgatc gaagttgcct tcgagggcgg
     1861 ccatggcaag ggcccgacgc tccgcggagt tctctttgtt caaagcgaaa tcgaagcaga
     1921 aatccactgc cgcgcgaccg ccaagttgac gcatggatcc gaacaaacgc ttgagttgtt
     1981 cgtcttgaag cgctgtcagt tgtgcggcga attgagcttc cgtgggcttg agtttgttgg
     2041 ccttgttggc ctcttccact gtcgatttga cggaatcgag ccacttggca gagatggtat
     2101 gagcagccac ggcgacgaca cgcttggagg cttcttcttt ggttggtttg tcaccattgg
     2161 cggcgatgat gcgcgcaagg tcggagattt tgcgttggtc gttgtggatc aaagaaggca
     2221 acatccgggc tgctggcgca ccgatgtact tgatgatctg ctcgacccca tacatttgcg
     2281 aagcgttatc gtaacgatgc tcgaagtcca cggtgaccca tgaagcaagg gcttgcatga
     2341 gagcttgtcg atgctgctct tcggagacga ttttccctcc gggtgagttt tcgcgaatga
     2401 gcaatgcaaa agccgcatcc ttgaaaggaa tcgatgtatc gggcggggcg ggctggttgg
     2461 cttgtgcgac cggcacgggt tttccgatct ccgagacgag cgcgggcacc atgcctgcga
     2521 tgatggtttt ccgaatttcc gagtcggtga gttgtgcgat cgcatccacc atgatgggga
     2581 tgccaatgcg tcgaccgcct ctaggtttca tgcgcacgag ggccaacgcg gcttccacac
     2641 gcaacgtggg gtcgtatttg tcatgggcga tgacagccac gagcttatcc ggtccgcgct
     2701 cagtcttttc ccagcgctgg atatcgtctt ggtcgacacg gcatccagac gaactcgcgg
     2761 cagtcatcat ggcgatagcc agggcggtta gaccccagca cgacagacgg ttggttcgca
     2821 tcgtctccct acttggctcc aacagggact cgcatgctac ccgacaaggc gtcgaagtct
     2881 caaacgaatt cccgatccaa gagcatcgag ccattcgata cccacggttc cacgggaatg
     2941 aaggggtttt tttggggggg attcggggct gggcgccggt tttttgaccc tttggggggg
     3001 tgggggatag gttggggctg ctttccgaaa tggcatcacc catgaccgca ccacggagcg
     3061 aaacgccccg gctggaattc gctgacgatc tccccaggca ggagagtcgc gcgtttcgct
     3121 atgggcggga agccgcttcc ctcgttctca tcgcgggtgc tgtgtttctg acgctggctc
     3181 tcgcttcttt ctcacacgat cccgccgttt cggaagtcac cggtaccaat tgggtcggcc
     3241 cggtcggcga ggcattggca aacgttttcg tctccacctt gggactcgcc gcctggatca
     3301 tccctgtcga actcgtcctc atctcgttgc ccttgtttcg taacaaaccc accatggcta
     3361 gcgtggcaag aatcggcggc gacaccgtcg tcattctcat cgtcgcttcc ctcatccacg
     3421 tctcgtttcc ccaatccacc gcgtttggat ccatgagcgt cggcggaacc atcggcgaat
     3481 tgttcggcga actcatgcga tccctgtttt ccaacgttgg ctcctacctc atcggactga
     3541 cggccatcgg tttggttcat cgctcgcgca tccttttctt tcatctcttt cgcccaacgc
     3601 gctggacaaa ccaccgaaca cgccgcgatg aaaagcgccg aggggttaca cgccatcaaa
     3661 cacgcgtggc atcgcgctcg aaccatcgaa aaagtccgcg ccgaacacgg agccattcgt
     3721 atcaccaccg ctgatcgtga tgatgccatc gtcgctatcg acaccgaggt agaagccgct
     3781 cgcgaacggg ccctcgccaa agctaccgag atgatcgagg ccttgcatca agaaccgctc
     3841 agccccctcg aagacatcga tgaaggcatc gttctcgaat cacccttgca ccttcagccg
     3901 gtttcatctc cgacgagtcc agaacccact atcgaagatg atctcgaaca ggatgcgcgc
     3961 gcccacgaaa gcgccccagt catcgacctc tctgcgtcca tgacccccat ggagtccaag
     4021 gccgcgacgt tgactcagcc cgcggctgag gaaacaccgc gacgcaagca acgtcaagcc
     4081 aaagggccca cgattgtgga taccacctcg gctacggaag tcgagaaggt caagcaaacc
     4141 gcggcgacat ccaccgtgcc tggatatcag atgcctcctt tcgatctttt cgatgaaggc
     4201 gaggctcctg ctctgatcat cgatgagaca ttgcaacaac aactcaacga caacgcggca
     4261 caactcatca cgacgctgaa tcactacggt gttagggggg agattcgcga aatccaaccc
     4321 ggtcccacgg tcacgaccta cgaaatggag cctgaggcag gcaccaaggt ttcgaaaatc
     4381 gccagcctgg ccgatgacct tgcgatgagc cttggatgcc aagtgcgcat catcgccccc
     4441 attcctggca aaaagcgagt cggattcgaa atacccaatc gcgaacgaaa ggccgtcaac
     4501 ctgcgcgaac tcatcgaaga ccgacgcttt caggaaatga aagcccccct tcccgtcgtg
     4561 ctcggaagag acatcgtcgg caaaccgttc tatgcggacc tgtcgtccat gccccacgtt
     4621 ctcgtggcag gtgcgaccgg cgccggaaaa agcgtaggac tcaacgtcat gctcgtgagc
     4681 ttgctgtgcc gtcgctcccc cgacgacttg cgattgctca tggtcgaccc aaaagtggtc
     4741 gaacttcaat cgttcgaccg catcccgcac atgctattgc ccgtcgtcac cgacacacgc
     4801 caagccgcaa acgcactgaa atgggcagtc gatgagatgg agcggcgtta tcaactcctg
     4861 gccaacgcag gcaccaagaa catcacgacg tacaacgcat gggtcaagcg ggttcaccaa
     4921 ggcgaggttc gaggtttcga acttcccaaa cccacggtcg tcacgtcgga agatggatcg
     4981 gttgtcgaat tcggtgcgga aaaagaacct cccaagcttc ccgaaaaact cccgtacatc
     5041 gtcatcgtcg tggatgagtt cgctgatctg atgatgcaac aaggcaaaga ggtggaagct
     5101 tcgattgcac gactggccca aaaagcacga gccgccggaa tgcacgtgat cctcgccacc
     5161 cagcgtccga gtgtcgatgt catcacaggg atgatcaaag ccaacttccc gtctcgcatc
     5221 gcgttcaaag tcacccagcg caatgacagt agaaccattc tcgatgaaca aggggccgaa
     5281 cacctgctgg gccgcggaga catgttggtc aaactcaacg ggtcgaacga aacacgacgg
     5341 gtgcagtgtc ccttcgtcag cgaagaagaa gtggagcgcg tgacgtcttt cctgcgtcaa
     5401 caaggagagc caacctacga tgacagcatc ctgctgccac gcggcgatga gacggaacca
     5461 gaagaggatg cggagcaaga cccgaagtac gatgaggccg tgatggtggt agctgaaaca
     5521 cgtcgctgct cgacctcatg gctccagcga aagctaggta ttggctacaa ccgagccgca
     5581 aagatcgtcg aaatgatgga aaagcgcggt cttgtcggac ccgcccaagg ggccaaggat
     5641 cgagaggtcc ttatcgaatc accatgaagc atgcaacgtg ccaacgcatt ttggggattg
     5701 acttggtcga cagaagtgac aagacaccag ccgctgtgct gaatgggtcg aagaaccaag
     5761 accttccgcc gcatggggag ccggggttgg acgaacaaga gagaacggaa ctcatctcca
     5821 ccattgtcac cgtcatccga cgtcggggag taccgcctca cgtgcatcag gcggcgttga
     5881 ctctcatggg ttggcttgcg cgacgaaaaa tttgtgagca cccctgtcag acgggggtag
     5941 ctgaggccca tcaacagtcg ctgcgcatga cctcgagtcg taggtagttt tttcactgct
     6001 cggtgatgcg atacagtttg ccgtcatccg agcctgccaa aagcacccca tcggacacga
     6061 tcaccactgg cgcgtcgacg tcgcctgccg tcatcgcctt ccaccggaac gcacccctcg
     6121 cggtgagtga gtaaacggca tcatcctgtg ctccgaagta cagattcccc tgggcatcct
     6181 ccacgggaga gccgtgaatt ccatactctt tcgcgccagt tccaggaatc gaaaagctcc
     6241 acaactgctc tccggtgtct ccacgcatcg ccacgactcg tggcgatggt ccatacacac
     6301 ctgccacaac gtcatgcgaa cgggttacgg tcaaccctcc gcgcacgtgc ccttgcaccg
     6361 tcgttttcca acgcacctgt ccggtctgcg tatcgagcgc gaccacggcg ccgccatccg
     6421 tacccacata cagcaacccg tattctccaa tggatggcgc gcaatccact tgttgttcca
     6481 atcgcgtggc aaaacgcacc acgcccgttc gacggatacc gtatacgcgc tggtcttgtg
     6541 ctcccacata cacggaaccg tcggaatcca cggccggtga ggaatagatc tttcgcttcg
     6601 cacggacacg ccacaccacg gttccatcca ttcgcgcaaa gaacagagtc tcgttcgccg
     6661 cgaagacgag accgtggtct ggggtgggag aagccgacgt atccgcatcc ccatccgtgg
     6721 caagagacca aagcacgttt cccgtggagg aaagactcac gaagcggtcg tcgtcggtgc
     6781 cgatgaacaa tcggtctttc cacaccaacg ggcttgcata taggcgatct tccagtggtg
     6841 ttttgaagac aacggttccg tcctctcgaa gaccgaatag atgaccatcg agagtcccaa
     6901 ccacgatggt tccattgggc agaactgcgg gggctgtttg aatcggagcg tgaaggtccg
     6961 ccaatacgga cagcgacggt gaagccgggc caacgaaagg gctgcgattg cgatgcgccg
     7021 ggtccaggtg aaacatggtg ggtggtccgg ccacgacaag cgccttggga tccaacggac
     7081 gattcggcgt cgaggatatc gccggagcca cgatccacga tcccacgggc aaagcctgtc
     7141 gtgcttgtgg gatcggacgt gtggcggcgc gatgcttgag cacggcaagg ctcagaaata
     7201 ccaaagcaac cacgctcgct acgacaatgg gttgcctgtt gagcttggtc atggctctct
     7261 catcatcagc gcttcaccgc gtgctttgct cgagttccca acgtaattcc tgattgcctt
     7321 gcgcaccggc aaaccgaaga ccaaaccaac gcgcttgatt ggaaatggta ggatgacctt
     7381 ccgcggtcac gatgggaaac cgaatgcgat acacgcgtcg ccatggcgac gtataaggga
     7441 aatacgttct ttcgatggga ccgggtttgc gaatcgtcac gatctcttct ggcgctgtct
     7501 cattacccac atcgtcgatc aatcggacaa tccaacctga atcaggtttg ttcaagtcca
     7561 cccaccgcca gttggtcccg taaagcgcaa cgaagaactg atgacgctcc tgggcatcgg
     7621 caagtgttcg ttcgagaagt cgtcggcgtt gatcgacggt cagacggtaa tcctgcgcgt
     7681 agcgaacgac gtaagcccat cgaaagtcgt aggattcgaa ggtagcggtg accgtgagca
     7741 ggtcatcgag ttccgacagg gcaatgagtt gatctgatct cgtccatttt tgaaggacgg
     7801 cttgataatc cgccgccaca tactcgcgtg gaccgggctc gagtcggact tgaggcggag
     7861 cgcaagcact cgccatcatg acgagcccca cagtccacag cagcacgggc cgaccgcgtt
     7921 tgagcatggt cggaattgtg ccacgaatga agcgaaacat gggcgtttga tccatcgagg
     7981 gttacgaagc gaagagtaaa gaagacggca acgacgaatg ttacaccgag aaagaccttt
     8041 tcttcacggt cttgcgtaac ttcttgtcac ccaaaacagt cacacttgca ccgaacgaac
     8101 tcacccaact cgcggcccac gcaagggaaa caggtccaaa gcgtacatcc agcatcccgt
     8161 cggtccgcaa caccacggac tgcgaggaat gccatacgca atggcgaaga cgttccgtca
     8221 gcgaagcatc gaaacggaca tgcacatcga ttgttttgtc ttcttgatcg gacagaaaac
     8281 agaagggtga atcaaataga tcgcgaacgg acaaggcttt gacccaatcg ggagcctccc
     8341 gttcgattgg ctcccacaag gtggattcgt tgggcaatcg gatcaattcg agcgtccacc
     8401 gatttttggg atccgtttca tgacagggaa agccaatcaa gacgataccc atatggtgat
     8461 gaaccacgat gccaagtggc caaaggggag cggttgacgt gacggtaggg atgagaaacc
     8521 gaagggcatg ggtacgcgat tgcgcatgcc gaatccaagc ctgacacacg cgagcatggt
     8581 gttcagcata gtggggaagg tttgggaaag cgatttggac gcaagacaaa gggttgacgg
     8641 tggagggagg aaagagtctg tccgaaagag cggaatcgag cggtgatgca tcggagcgaa
     8701 agcaggagga aggagggagg gaggagggag aagacagcac ggcacgtacg agaacgcgtc
     8761 cgcggtcgcg ttttttttcg acgtcaaaac cgaggagttg ctcggcgtgt tggagataat
     8821 ttcgcacgga acgaacggta caaccggctt gttgagccaa ttcgagatag gtgagcggtc
     8881 gaccttgagc gagggcgtgt tggagacgtt ggagaatggt ggcagagacg gctcttcctg
     8941 aggtggcccg catggggctc atggttcacc ggagggaaag ctttcgcaag ggaatgatgg
     9001 agtgatactg gtggaaggaa aggattcctt ccggaaagta cgagacgtag tggaagggag
     9061 ccgttgctag cgcttggagt attggaagcg tttgcgagca cctggctgac cgtacttttt
     9121 gcgctctttt tctcgcgcat cgcgggtgag atagcccgcg cgcttgagca tgccccgacg
     9181 ttccgggtcc tgttcgatga gggcacgggc aatcccatgg cggagtgctt ctgcttgagc
     9241 gctatgacct ccacctttta ccgtagccca gacgtcgatc tcatcgagag cttcgagcag
     9301 ttcgagagac tgacggcaaa tcatgcgggt ggtttctctt tcgaaatagt catccgcaag
     9361 ggtgttattg acgatgaact gaccggatcc tgggctgatc cagacacgag cgacggcgct
     9421 ctttcgtttc cctgtggcgt agaatcgatg ttccgtgggc atgaggatca gatctcgagg
     9481 ggttgcggtt tctgcgccgc gtgaggatga tccgcggtag cgtatacctt gagcttaccg
     9541 atcatctgac gtccgaggac gtttttcggc agcatgtttt tgacggcttt ctgaatgggc
     9601 aactccggct tgcggttcat gagcaagcgg tagggttccg cacgaaaacc gccgggggaa
     9661 ccgctgtggc ggtagtaatg ttttttgtcg agtttggaac cggtcagacc gaccttggca
     9721 gcgttgatga cgatgacaaa gtcaccggta tcgacgtggg gggtgaaggt cggcttgtgc
     9781 tttccacgca agatcttggc gatctcactg gcgagacgcc cgagggactt gccttcggca
     9841 tcgacgaccc accactccct ggaggcagcg gcctcagcgg gtttggcgga gaacgttcgg
     9901 ttcatggcat ggtgcctttc ctacaacttc agcgcttcca gatgatcatc atccggatga
     9961 tgagcccgtc atcccggctc aatcaacaat ggggtcgcgg tttttacagg gagccacaac
    10021 gattgtcaag cacagtccac gatcattttt gccagccatc ggctcgaagg ccgtcgactc
    10081 atggcatacc cagcagttgg ctcatcgagg cttcgtccgc gggggtaaat gggtactggt
    10141 cgaattcggc actgcttgcc caacgaaaag catgaacatt tcgcggactt ggctctccac
    10201 cggtcaggcg gcactcgtaa agatacaagt ccaccacata acgctcgtag gggtgggata
    10261 cgaaagagat gagctgaccc acggaaatat cgacccccaa acgatgacga acctcccgtt
    10321 gaagcgcagc cgtatccgtt tctcccggct cgacccgccc tcccggaaac tcccacatca
    10381 gcggcagcac cgctgtcgga cgacgttggg taatgagata gcggccattg ttttccacaa
    10441 ccgcagccac cacacgaacg gtctggatgt tctggtgatc catgcttttc gtcactccca
    10501 tgcacaaaca gactccgccg tttggatcgg atcgtttcac ccaccgacaa cgttttgcgg
    10561 aacgaaactt cggtcagtgg ccaacacgaa atagctgctg acccatcaac aagatatcgc
    10621 cgtcttgtac ctcacgctca ctcatcaacc gaatgaacga accgttggaa cttccaagat
    10681 ccgtgagcag cacttcttca tcgtggaccg tgagtgaaca atgaagtcca gacacgtaac
    10741 catcttcagg gaacagcaca tggccacgtt cgcgcccaat gtgaacgccc agtttcggta
    10801 ccggaaatgc gttaccgtcc atctgacgcc cggtcaccag cacaatccgc cctatatagc
    10861 ccgtcgaagg acttcccatt ttctccacac cgtcgatcgg cggttcggga tggagctttt
    10921 cgaaacgaat gatctcttgc ccaatgcgga aatactgacc gtcggccacg gactccggcg
    10981 tgtcacgcat gagcttgaga tacgtgccgt tgagcgaacc ggcgtcttcc acgaaaagct
    11041 ttccccctcg cagactgagt ttcgcatgct gcggagataa atacgaatca ccaccgaaaa
    11101 ttccaccggt gcttcgaccc acgagcgtga cgtcggaagg cacgtcgaat gttcccgcat
    11161 cggttccatc cgcgcgcaac acagtgagtt tccacgtcgc cgcggaagcc gaagtcaccg
    11221 cgggcgctgc tggaacagaa acgactttgg tgaggttgta gccacaagac gcacagaact
    11281 tgaacgccgt agagttgaca tgactacact gcgggcaagc caccgtttcg cccgtggcac
    11341 tcgccatcgg tgcatctgca acaatgggtg cgggagctgg ttcagccact ggcgcgggag
    11401 acaccgctgg cggaacgaaa ggtgctggcg acggttccgc cacgggcctc ggtgccaccg
    11461 ctggcggaac gaaaggagcg ggcgatggtg ccggagcagg tgcatgataa gctgccactg
    11521 ccggtgaact cttaccaccc gatactgcct tcatcccctg cgggggcgtt cgaggcgcga
    11581 acggcttggg tgccgcatca cggggaagtt ccgctccgca accgagacag aacttatagt
    11641 gatcctggtt ctccttccca cacttggaac acgtgatcac cggttacctc ctaatcgctt
    11701 gcgcacattg cgcccttgct attgccgttc acgttcttgg ctgaaccttg gcccatccaa
    11761 tcgaaaacct acgcgattat cgccgcgccc gtggccaggt ca
//
LOCUS       DAOSOR010000112        11789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5401_length_11789_cov_4.541248, whole genome shotgun sequence.
ACCESSION   DAOSOR010000112 DAOSOR010000000
VERSION     DAOSOR010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11789
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5401_length_11789_cov_4.541248"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PK710_08215"
     CDS             <1..516
                     /locus_tag="PK710_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09740.1"
                     /translation="RDGKAHGFQVVALDSRAGVDAMLESKTSVFRCEPPAPFVFPEDG
                     LLRPVQSLDSLNAWQLSLGQDLALMTPSEFSQYVEGPALPESPLWIRLPTSYEHATSY
                     AIVDSGLLRPVAARTLAAWRVSPDSLRTATVEELSLPRGSTFAQTPFVVQKTDGTLFI
                     LDTNPVSPVLP"
     gene            525..1568
                     /locus_tag="PK710_08220"
     CDS             525..1568
                     /locus_tag="PK710_08220"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT09741.1"
                     /translation="MKTEKSVIRWGILGCGNVAEVKSGPAFQKTPHSQLVAVMRRQGD
                     LAQQFAQRHGVPRWYDNAEALIHDPEVDAVYIATPVDNHANLALQVAKANKPAYVEKP
                     MARHFPECQAMLEAFTHARLPLFVAYYRRKLPRFLTVAALLEQGALGTITAIRYHYGS
                     ASHRKPLSPLPWRLDAARAGAGLFLDLGCHVLDILDFLLGPVHHIQGSAYNVASTYDV
                     EDVVLMTFQVGKAIPGVASWNFAQQQHEDCLDIEGTDGHLRLSVFGDSPIRLTRGKEH
                     RDLRIANPPHIQQPLIESIVDDLLGRGTCPSTGTTAARTSLVMDTVLDGYYGGRQDRF
                     WERPESWPGRRTW"
     gene            complement(1619..2470)
                     /locus_tag="PK710_08225"
     CDS             complement(1619..2470)
                     /locus_tag="PK710_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09742.1"
                     /translation="MAAWLTYIKERFPIPVYLLLAGGMTTTGVLAGQAPLSWLAVAVG
                     LVGNLLFLALLRLMDEYKDYDKDRVAHPERPLPRGLLEVATVRRVIHAGALVMLGYSG
                     VAYFYAGFPAASLYLISTVFLWLMFKEFYIGSWLARIPILYAITHQVVMLPLVAFCTT
                     LGRPESIGDPMTWITASMALGSFFTYEICRKLDPEAPRILDTYLIHHGRIATSFFVVC
                     TCALAAWASYRVGMHKLLWPANAIVLASLSLLFVAPRKYKVVEAIATLSLVLHLWGHT
                     LRYFIGC"
     gene            complement(2455..3870)
                     /locus_tag="PK710_08230"
     CDS             complement(2455..3870)
                     /locus_tag="PK710_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT09743.1"
                     /translation="MNCVDLFVQQAKRKPNTVALWSPDGTRMTFEELLAMAGRTQRAL
                     RDAGVKPGSSVLVVELPGPRLYAIVLAVFAMGATVIAVEPWLPVGEVDRVIRFMRPTL
                     FIGGALGMAWGARVPAVRAISRWKASSRVLSARGAPLSIEDTSPETPALVAFTSGTTG
                     QPKGVVRPQGYLIHQHDIYTRSLGLTHHEGPDLCIFANFVLANLASGRPSLLVSPRWS
                     FKQLRAIDDLPDELQPRTLSCGPAFLAQILRNARLDKLASVHVGGALSDVSLWENAFD
                     KWPDTHFLHVYGSSEAEPVAVLDARKAVAVSRARGFHQVLCVGKPVDAIRSRLEPNSL
                     WVTGPHVCRWYVGNDRENLVHKRVDEHGNVWHDMGDRIEVRDDLWWYAGRASQPAEDF
                     SWEQSVYHELGHSRAFIHRTNPGSVYLVGEGLRQNAERIKQQYRGIDRLICCPIYRDR
                     RHRARIDRRTTLRKGAPWLPG"
     gene            4035..4898
                     /locus_tag="PK710_08235"
     CDS             4035..4898
                     /locus_tag="PK710_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09744.1"
                     /translation="MAMIVLGFRSYATDQREREFSEQHTPLAASLAKHRGLLARASAL
                     QPYFREMSSEITPSRCRELMDLIRAHDNRDPFDENPRCEEDPPSPMDLCDAARVFAEV
                     AGGETQLSRPVGVLLQSRKHGQERGEERLFVTTLFLSEKHALLEMHQLAFPAPSMDGK
                     PLRWLVRSLGDDGLFERCEEAGRIDVRATTPDGTLRMHRREEGKSNIIEWLRESRSVG
                     TSIYLWPSRGVRYEAATGVGCEPATWSELWIVKQAGSNVCTRVRRHDQGSVQTWQRGT
                     GCESAGGQWGK"
     gene            complement(4984..5559)
                     /locus_tag="PK710_08240"
     CDS             complement(4984..5559)
                     /locus_tag="PK710_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09745.1"
                     /translation="MAAGFSPNWFIAIPIDPEGWFAPTPSPPDGIRLFHQEDLHLTLA
                     FLGSVSEQQARAGWEALRWNLAPTTVTLGAVVPMGAPGRYSALSATLEQGRATIEQAI
                     GACRDAICDAAGARRESRSPKAHITIARPSRNASDSTRAGGLRWAQRIDYHQPSVPLH
                     TIALYTWANDRKVRQFRTVLRASWQELSGNR"
     gene            5646..6086
                     /locus_tag="PK710_08245"
     CDS             5646..6086
                     /locus_tag="PK710_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09746.1"
                     /translation="MKLAFAFAVMLLAATSLGCKKRSKKGVEDTTSDSVAVASAGAGV
                     SSNCRKLCLLVGKCTEKDGKCYAGSKAECEASVGCKSAGLCSLKEERCVALTDAECEQ
                     SDFCRIKGFCLAVDGKCVNRSEPPSSASAAVSAPPAASAAASSQ"
     gene            6138..6650
                     /locus_tag="PK710_08250"
     CDS             6138..6650
                     /locus_tag="PK710_08250"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HOT09747.1"
                     /translation="MQLFEMLVRLRGLSPTFSRVLLTRLVPRIIPLSDGMGVTVQEWS
                     AERCVLELPFRRKNRNHLGSMYFGAQMTLADLAVGLLLFQHFPMDRYGGVIKRVEADF
                     RAKGKGALRCVAELPDDVADVLNDARVNDSGKAEAWVPMQLTGPDGKTVTEVRTLVAI
                     KRFGPPEKQA"
     gene            complement(6647..8107)
                     /locus_tag="PK710_08255"
     CDS             complement(6647..8107)
                     /locus_tag="PK710_08255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOT09748.1"
                     /translation="MNSAVRFNRPMTNHEDSNDRGSQEKQRVALTSVIAALFLVGGKL
                     AVGLWTNSLGILSEALHSGLDLVAAGLTFWAVRVSSRPADDKHTYGHGKVENLSALFE
                     TLLLLATCVWIIFEASSRLLKKEADAVDASIVAFVIVVASILIDISRSRALKRVADKY
                     DSQALHADALHFSTDIWSSAVVLVGLIGVRLAEPLHAPWLRKADAFAALGVAGIVVWV
                     SLKLGKTAIDSLLDSVPPQLPETIRLAALTVDGVMSVRQVRVRKAGPDFFADVTVVTQ
                     ANTYLEQAHEIAHQVDDAIQRHLPKADVVVHVEPEHISEDTLPQMVHLIAHRHGILAH
                     DIVVRHGLEQSVLEAHVELPGSLRVDAAHAKVTEFEKEILNARPDLHHVLTHIEPIER
                     ATCTIPIDASRVHALIEELATSSSFGAWHPSHIEVCGTENTVSVSFHCIVNGEETVGY
                     AHELSERLERTLRERMPELGRITIHIEPRQDGIDNP"
     gene            8167..8964
                     /locus_tag="PK710_08260"
     CDS             8167..8964
                     /locus_tag="PK710_08260"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT09749.1"
                     /translation="MTYSALLMVFPFVFGVGCVGAKPAAGEVREPAHTTLPTNMPSSP
                     QARDVEPVVEPKNVESGVPDPVQPPAVAQPCPEDMVDVASVCIDRFEAPNEKGKHPLL
                     MQSAHDAEAWCKERGKRLCNEDEWVRACEGPQHLPFPYGETYQQGVCNDDKTWKAVQW
                     GRLGRWPDDVARAEANRLDQSEPSGMREGCRSAEGVYDLTGNAGEWVIRTRDNPTNYS
                     HVVKGCYWGKCFRPPHTPSCEYVNYGHEAGFRSYEFGFRCCKDRGAS"
     gene            complement(9036..9713)
                     /locus_tag="PK710_08265"
     CDS             complement(9036..9713)
                     /locus_tag="PK710_08265"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOT09750.1"
                     /translation="MSPGILLVGLNRHAVLLVMMGTIASLCGCRSESSVHQQPLAVGT
                     VSASTSAAPPIAPTPPLTDEHATLQGYEMAKVHRVVLTSQGSAVLLQSQDSGKILPIF
                     VGGTEALSIDLRNDSKRFQRPLTHDLLDELVVRLGGKIRKVHVDALRGHTFIGRVFCQ
                     QHDQWFDVDARPSDAIALALGNNVPIYVSTRVFEQAGVSPNDISGTDPDELPSSPMGR
                     PKDPMQL"
     gene            9829..10971
                     /locus_tag="PK710_08270"
     CDS             9829..10971
                     /locus_tag="PK710_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HOT09751.1"
                     /translation="MNASPRKVEFFRHDLGEEEIASFRDTLSSLFLTTGPRVAEFETL
                     LATYLGVAHCVGVTSCTHGLHLALLALGIGPGDEVITTPLTFVATANAIVYAGATPVF
                     ADVDPATGLLDPKAVEAAITSRTKAVIVVHLYGQMVDMKTFRALADRHHLALIEDSAH
                     GVEMRRDGIAPGQQGDAAVFSFYATKTMTCGDGGAVAVRDTNLDRRLRQLRYHGISKD
                     VMARHATPYAHWDMVELGYKAGLSDIDAALLIPQFGRLEQRRHKRELVVRGYRDRLEA
                     TPGLSFLRVEGISSHHLCTVLTPKERRDDLLRDLGQAGIGVAVNYRAIHGLTYYREQH
                     GFRKGQFPHAEAIGDRTISLPLWPGLPEDDIDYVAERLRDLMRVGG"
     gene            10977..>11789
                     /locus_tag="PK710_08275"
     CDS             10977..>11789
                     /locus_tag="PK710_08275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT09752.1"
                     /translation="MGVGMDATGHAVGHPVGADGIVYSVVVPVFNEEANIEAVVRRVT
                     PVLESEGQPYEILFVDDGSKDRTPQLLAAQCQTNSHVRAIRFSRNFGQEAAVQAAYLY
                     ARGQWIVQMDGDLQNPPEEIPKLLRLRDEGYDIVYGVRTGRRDPWFRKFASQTMRWSM
                     RRLLHIELPKDISTFRLMRAATAKTLAQFPERQKFLSALACWMGAKHTTVDVAHAARQ
                     AGHTKYNLGKLVNHTFDLVVGFSSRPLRMIGVLGFGAGVIGFGFAGWAIVEKL"
BASE COUNT         2541 a         3241 c         3495 g         2512 t
ORIGIN      
        1 cgtgatggga aagcccatgg atttcaagtc gttgccttgg attcgcgggc aggggtcgat
       61 gcgatgctgg aaagcaagac ttccgttttt cgctgtgagc cgcccgctcc cttcgtgttt
      121 ccagaagacg gcctgcttcg tcccgtccag tccctcgata gcctaaacgc ttggcagctc
      181 agcctcggac aagacctcgc cctgatgaca ccctcggagt tttcccaata tgtcgaaggc
      241 ccagctctgc ccgaaagccc gctttggatt cgcttgccga cttcctatga acacgcaacg
      301 tcttatgcga tcgtggattc cggcctgctc cgccccgtgg ctgcccgaac cctcgcggcc
      361 tggcgcgtga gcccagattc cttgcgcacc gctactgtcg aagaactctc cctgccccga
      421 ggttccacct tcgcccagac cccgtttgtc gtccaaaaaa cagatggtac actctttatc
      481 ctcgacacca accccgtttc acccgtgttg ccctaagctt ccctatgaaa acggaaaaat
      541 cggtcattcg ctggggtatc cttggatgcg gcaacgtcgc cgaggtcaaa agcggccccg
      601 cttttcaaaa aacaccccat agccaactcg tggccgtcat gagacgccaa ggcgacctcg
      661 cccaacaatt tgcgcagcgc cacggggtgc cgcgatggta cgacaacgcc gaagccctaa
      721 tccacgaccc cgaagtcgat gcggtctata tcgctacccc cgtcgataac cacgccaacc
      781 tcgcgcttca ggtcgccaaa gccaacaaac ccgcgtatgt cgaaaaaccc atggcccgtc
      841 actttcccga atgccaggcc atgctcgaag ccttcaccca cgcccgcctc cctctatttg
      901 tcgcatacta tcgccgtaaa ctgccccgat tcttgaccgt cgccgccctg ctcgaacaag
      961 gcgccctcgg aaccatcacc gcgatccgct accattacgg ttctgcttcc catcgtaaac
     1021 cgctttctcc gctgccctgg cgtctggacg ccgcccgcgc gggagcagga ctctttctgg
     1081 atctgggctg ccacgtcctc gatattctcg actttctctt ggggcctgtc caccatatcc
     1141 aagggtctgc ttataatgtt gcgtcaacgt atgatgtgga agatgtcgtg ctcatgacat
     1201 ttcaagtggg aaaagccatt ccgggtgttg cctcctggaa cttcgcgcag caacagcacg
     1261 aagattgcct ggatatcgag ggaaccgacg ggcacctacg tctttctgtc ttcggcgatt
     1321 caccgattcg tttgacgcga ggaaaagagc atagggatct tcggatcgcc aatccgcccc
     1381 acatccagca gcctttgatt gaaagcatcg tcgatgattt gcttggacga gggacgtgtc
     1441 cgagcacggg gacgacggcg gctcgaacgt cgctggtgat ggatacggtt ctcgacggct
     1501 attatggagg ccgacaggat cggttctggg agcggccgga gtcgtggccc ggacgacgaa
     1561 cctggtaaat tgtccgtggt ttaccgcgtt tgtgcttcga acagacttca aggatctttc
     1621 agcatccgat gaagtatcga agcgtatgcc cccatagatg caaaacgaga gagagcgtgg
     1681 caatcgcttc cacaaccttg tactttcgcg gcgctacaaa aagcagcgat aggctcgcca
     1741 ggacgatggc attggcgggc catagcagct tgtgcatccc cacgcgataa gacgcccagg
     1801 cagcgagcgc gcaggtacaa acgacgaaaa agctcgtggc gatacgcccg tgatgaataa
     1861 gataggtgtc caggattctc ggcgcctccg gatcgagttt tcggcagatc tcgtacgtaa
     1921 aaaacgagcc cagagccata cttgccgtga tccacgtcat cggatcaccg atggattcag
     1981 gacggcccag cgtggtgcag aacgccacga gcggtaacat gaccacttgg tgggttatgg
     2041 catagagaat gggaattcgg gcgagccagg aaccgatata aaactccttg aacatcaacc
     2101 acagaaacac ggtgctgata aggtacagac tcgcggcggg aaatcccgcg taaaaataag
     2161 caacaccaga ataaccaagc atcaccagcg cgcccgcgtg gatgacgcgt cgaacagtag
     2221 cgacttcgag caaaccgcgg ggcaggggac gctcgggatg agccactcgg tctttatcgt
     2281 aatctttata ctcatccatg agccgcaaaa gagcgagaaa taaaaggttt cctacaaggc
     2341 ccaccgcaac cgcgagccac gaaagcggcg cttgccccgc cagcactccc gtcgtggtca
     2401 tgccgcccgc gagcagaaga tacaccggaa tgggaaaacg ttctttgata taggttaacc
     2461 aggcagccat ggtgctccct ttcgaagcgt ggtacggcga tcaatacgcg ccctatgacg
     2521 tcgatcgcga taaatcgggc agcagatcaa tcgatcgatc cctcgatatt gttgcttaat
     2581 tcgttccgcg ttttggcgta gcccttcgcc gacgagatag acactccccg ggttggtccg
     2641 atggatgaaa gcgcggctat gtccaagctc gtggtatacg gattgctccc acgaaaaatc
     2701 ctccgcaggc tggctggcgc gcccagcgta ccaccataga tcgtcgcgga cctcgatgcg
     2761 gtcgcccatg tcatgccaga cgtttccgtg ttcatcgact cttttgtgga cgaggttttc
     2821 cctatcattg cccacatacc atcggcaaac atgggggccg gtaacccata gggagttggg
     2881 ttcgaggcgc gagcggatgg catcgaccgg cttgcccacg caaagcactt gatgaaatcc
     2941 tcgcgctcgg ctgacggcca cggcctttcg agcatcgagc accgccaccg gttccgcctc
     3001 ggaactccca tacacatgca gaaaatgcgt gtcaggccac ttatcaaagg cattttccca
     3061 taaggaaacg tcggaaagcg ctccgcccac atgcacggac gccagcttat ccaaccgggc
     3121 gtttcgcagg atctgcgcga gaaatgccgg cccacaactc aacgtccgcg gttgcagctc
     3181 gtctggcaaa tcatcgatgg cccgcaattg cttgaaactc caccgcggtg acaccagcaa
     3241 cgatggacga cccgaggcca gattcgctaa aacgaaattc gcgaaaatgc atagatctgg
     3301 gccctcatga tgcgtgagcc ccagcgaccg tgtgtaaatg tcgtgttggt gaattaaata
     3361 cccttgtggg cgaacgacac ctttgggttg gcccgtggtg ccggaggtga aggccaccag
     3421 cgcgggcgtc tcgggactcg tgtcttcgat agaaaggggt gccccgcggg cggaaaggac
     3481 gcggctcgaa gctttccacc gagaaatggc gcgaacggcg ggaacccggg ctccccaggc
     3541 cataccaagg gctccaccga tgaatagtgt gggtcgcatg aaccggatga cacgatcgac
     3601 ctcccccacc ggcagccaag gttcgacggc aatcaccgtg gctcccatgg cgaaaaccgc
     3661 aaggacgata gcgtataggc ggggccctgg cagttccacg accagcacgc tcgaaccagg
     3721 tttcaccccg gcgtcgcgaa gcgctcgctg ggttcgcccg gccatcgcca gcaattcttc
     3781 aaacgtcatt cgggtcccgt cagggctcca caaagcgacg gtgttgggtt tgcgcttggc
     3841 ttgttggaca aacagatcga cgcaattcat gcggactccg gatcgggttc ttccagacga
     3901 tcttgttgag cagcccttgc atggttttgc tgcatcggca atcgttcgtg ccaaggtaga
     3961 gcttggtagc tatggtctta tccccttttt cgggtcgaaa agcagcttgg tcggcggttg
     4021 ctgcggtagt tgtcgtggca atgattgtct tggggttccg atcctatgcc acggaccagc
     4081 gagagcgtga gttttcggaa caacataccc cgctggcagc gtcgttggca aagcatcgag
     4141 gcttgttggc gagggcgtcg gcgcttcaac cctactttcg agagatgtct tcggaaatca
     4201 caccatcgcg atgtcgtgaa ctcatggacc tcatccgcgc ccacgataac cgggacccgt
     4261 tcgatgaaaa tcctcgttgt gaggaagacc caccctcgcc gatggacctt tgtgatgccg
     4321 cgcgggtttt cgcagaggtc gcaggtggag aaacgcagct ttctcgtccc gtcggcgtgc
     4381 tgctacagtc gcgaaagcac gggcaagaac gaggagaaga aaggcttttc gtcacaacgc
     4441 tgtttctatc cgaaaaacat gcgctgctgg agatgcatca actcgcgttt ccagctccgt
     4501 cgatggatgg caaacccttg cgctggctcg ttcgttccct gggcgacgat ggtcttttcg
     4561 aacgatgcga agaagcgggg cgaatcgatg tacgggcgac aacgcctgac ggtaccctcc
     4621 gtatgcatcg tcgagaagaa ggaaaatcga atataatcga atggttgaga gagtcccggt
     4681 ccgtagggac cagcatttat ttatggcctt cccgtggtgt tcgatacgag gcggcgacgg
     4741 gagtcggctg tgagcccgcg acttggtcgg agctttggat cgtaaagcaa gcggggagca
     4801 acgtttgcac acgagtgcgt cgccacgatc aaggctccgt gcaaacgtgg caacggggaa
     4861 cgggatgtga atccgcgggg gggcagtggg gcaagtagat tgcgtttgcg tacggcgttg
     4921 gcgttgcgag cgggattggg atcgagatgt cgacgactac gttggtgcgc acgcaaacgt
     4981 ggtttagcgg ttgccggaca attcctgcca cgaggcgcgc agcacggtac gaaactggcg
     5041 aactttgcga tcattggccc aggtgtacag ggcgatggta tgaagaggaa cggaaggttg
     5101 gtggtagtcg attctctgcg cccagcgcaa tccaccggca cgtgtcgagt ctgatgcgtt
     5161 gcgggaaggt cgtgcgatgg tgatatgcgc tttgggagaa cggctttctc gcctggcacc
     5221 agcggcatcg cagatggcgt cacgacaggc tccgatggcc tgttcgatcg tagcccggcc
     5281 ttgttccaag gtcgcggaca gcgcggaata ccgtccgggc gcacccatcg ggacgacggc
     5341 accaagggta acggtggtcg gtgcgaggtt ccaacgaagg gcttcccaac cagcgcgcgc
     5401 ctgttgctcc gacacggagc cgagaaaagc aagcgtcaag tgcagatcct cctgatgaaa
     5461 aagacgaatg ccgtcgggag gggagggggt cggcgcgaac cagccttctg ggtcgatggg
     5521 gatagcgatg aaccaattgg gagaaaagcc tgccgccatc ccatcatcga aacatgattc
     5581 accaatcgat gtcaaagcgc ggcgtgacag gtgcgacgag cgatggtttc ggttatggtt
     5641 cgaccatgaa acttgcgttt gcttttgcgg tgatgcttct ggcggcaacg tcgcttggtt
     5701 gtaaaaaaag atctaaaaag ggagtggaag acacgacatc cgattcggta gcggttgcat
     5761 ccgccggggc aggggtatcg tcgaattgcc ggaaactctg tctgttggtt ggcaaatgca
     5821 cggaaaagga cggcaagtgc tacgcgggtt ccaaggctga gtgcgaggct tccgtggggt
     5881 gcaaaagcgc gggattgtgt tccttgaaag aggagaggtg cgtggcgcta acggatgcgg
     5941 aatgcgaaca atccgatttc tgtcggatca aaggattttg ccttgcggtg gatggtaagt
     6001 gcgtcaatcg gagcgaaccc cccagttccg cgagtgcggc ggtgtccgcg ccccctgcgg
     6061 ctagcgcagc cgcctcgagc cagtaacgca gatcacattg tcgtgcgttt cgaagcgtgg
     6121 cccggtacga tgacgccatg cagctcttcg aaatgctcgt gcggcttcgt ggattgtctc
     6181 caaccttttc tcgtgtgctg ctgacacgcc tggtgccccg catcatcccg ttgtccgatg
     6241 gaatgggggt aaccgtgcaa gagtggtccg ccgagcgatg cgtgctcgag ttgccgtttc
     6301 gtcgcaagaa tcgcaatcat cttggaagca tgtattttgg cgcccaaatg acgctagcgg
     6361 atttggccgt cggattattg ctgtttcagc attttcccat ggaccgatac gggggagtca
     6421 tcaagcgtgt cgaagcggac ttccgggcaa aaggcaaagg cgcgctgcgg tgcgtggcgg
     6481 agcttcctga cgatgttgcg gacgttttga atgacgcaag ggtcaacgat agcggcaaag
     6541 ccgaggcgtg ggttcccatg cagctcacgg gcccggatgg aaaaacggtg acggaagtga
     6601 gaacccttgt ggccatcaaa cgctttggtc caccggaaaa acaagcctaa gggttatcga
     6661 ttccatcttg tcgaggctcg atatggatgg tgattcgccc gagttcaggc atgcgttcgc
     6721 gaagcgttcg ctccaatcgt tccgatagct cgtgagcgta acccacggtt tcttccccgt
     6781 tcacaataca gtgaaacgat acagaaaccg tattttcggt tccgcatacc tcgatatggg
     6841 aggggtgcca ggcgccaaac gaagatgatg tggcaagctc ttcaatcaga gcatgcaccc
     6901 gggaggcatc gattgggatg gtacaagtgg cccgttcgat gggctcgata tgggtgagta
     6961 catggtggag atccggccgg gcattcaata tttccttttc aaattcagta actttggcat
     7021 gtgccgcatc gacgcgcaga gaaccgggca gctccacatg cgcttcgagt acactttgct
     7081 ccaggccgtg gcgaaccacg atatcgtgag ccaggatacc gtggcgatgc gcgatgagat
     7141 ggaccatctg cggcaatgta tcttccgaga tgtgttcggg ttcgacatga acgacaacgt
     7201 ccgctttggg caagtgccgt tggatggcgt cgtcgacctg atgagcaatt tcgtgggctt
     7261 gttccaaata cgtattcgct tgggtgacga cggtgacatc ggcaaaaaaa tcgggtcctg
     7321 ctttgcggac tcggacttgt cgcactgaca tcacgccgtc gaccgtgagg gcggcgagtc
     7381 gaatcgtttc gggaagttgg ggaggaacgg agtcgagcaa ggaatcgatc gcggttttgc
     7441 caagcttcag gctcacccag accacgatgc cggccacgcc cagggcagcg aaggcatcgg
     7501 ctttgcgtag ccatggcgca tgcaaaggct cggccagacg taccccgatc aagcctacga
     7561 gcaccaccgc cgatgaccaa atatccgtgg aaaaatgcaa agcatcggca tgcaacgcct
     7621 ggctatcgta cttatcggcc acccgtttca aggcccgcga tcgcgagata tcgatgagga
     7681 tcgaggcaac gacgatgacg aacgccacga tgctggcatc gacagcatcg gcttcttttt
     7741 tcaatagccg cgagctggct tcgaagataa tccacacaca ggttgccagc agcaacaagg
     7801 tctcgaacaa cgcggaaaga ttctccactt tgccgtgacc gtaggtatgc ttatcgtcgg
     7861 cggggcggct cgatacgcgg acagcccaaa atgtaagccc cgcggctacg agatcgagtc
     7921 ccgaatgcaa cgcttccgac agaatgccca gactgttggt ccataggccc accgcaagtt
     7981 tgccgcccac aagaaataaa gccgcgatga ccgaagtcag cgcaacacgc tgcttctcct
     8041 ggcttccgcg atcgttacta tcctcatggt tggtcatcgg acggttgaat cgtacggcag
     8101 aattcatccg tagcccatgg cttcagaagt cgtcgtggca tgattccatc gtggtaaagt
     8161 acggtcatga catattccgc actacttatg gtgtttccat tcgtgttcgg ggtcggttgc
     8221 gtgggggcaa aacctgcggc aggcgaagtg cgtgagcctg cgcataccac gttgcccacg
     8281 aatatgcctt cctcgccgca agctcgtgac gtggagccgg tggtggaacc gaaaaacgtt
     8341 gaatcaggcg ttcctgaccc cgtgcagcct cctgctgtcg ctcagccctg tcctgaagat
     8401 atggtcgatg tggcgtcggt gtgtatcgat cggttcgaag ctcccaacga aaagggaaaa
     8461 cacccgctgt tgatgcaatc ggcccatgat gccgaggcct ggtgcaaaga gcgtggaaaa
     8521 cgattgtgca acgaagacga atgggtgcgc gcttgcgaag gcccgcaaca ccttcctttc
     8581 ccttatggtg aaacctatca gcagggagta tgcaacgacg acaagacatg gaaggcggtg
     8641 caatgggggc gacttggacg atggccggac gatgtggccc gggcagaggc aaatcggctc
     8701 gaccagtcgg agccgagtgg aatgcgtgaa ggttgtcgat cggcggaggg ggtttatgac
     8761 ctgacgggta atgcgggaga gtgggtgatt cgcacacggg acaatcccac caactacagc
     8821 catgtggtca aaggttgcta ttggggaaag tgctttcgtc ctccccacac gccgtcctgt
     8881 gagtacgtca attacggtca cgaagctggg tttcgaagct atgaatttgg ctttcggtgc
     8941 tgcaaggacc gtggtgcctc ttgacccttg ctgctggttt gcgtgggaag atactccctg
     9001 attcgtatct cctgtgattc gtggcacagc tcacatcaaa gctgcatcgg gtctttcggt
     9061 cttcccatcg gagacgaagg aagctcgtcg gggtccgtgc cggaaatatc gttcggtgaa
     9121 acgcctgctt gctcgaaaac gcgcgtggac acgtagatcg ggacgttgtt tcctagcgcg
     9181 agcgcaatgg catccgatgg tctggcatcc acatcgaacc attggtcgtg ctgttggcaa
     9241 aacactctgc caatgaacgt atggcctcga agggcatcca catgtacttt gcgaattttt
     9301 ccaccgagcc tcacgacgag ttcatcgagc aagtcatggg tgagaggtct ctggaacctc
     9361 ttggaatcat tacgtaaatc aattgaaagg gcctcggtgc ctcccacaaa tatcggcaga
     9421 atttttcccg aatcctggga ttgcaaaagc acggcactcc cttgagaggt cagcacgaca
     9481 cgatgcacct tcgccatttc gtatccttgc agcgttgcgt gttcatcggt gagggggggt
     9541 gtcggcgcga tcggtggcgc cgcgcttgta cttgcggaga ccgtgccgac tgccaagggc
     9601 tgctggtgga cgctggattc agagcgacag ccgcacaaag acgcgatggt tcccatcatc
     9661 acgagcaata ctgcgtgtcg gttcaagcct acgagcaaaa tccctggact catgatggca
     9721 tggtgacccg aagcgcgggg ccttgctacc gtccggaagg gatcccttcg caattgcccg
     9781 caccatgacg tgactgtcaa gccgttccgt attatggcgt cggtcgtcat gaatgcctcg
     9841 ccgcgcaaag ttgaattttt tcgccacgat cttggcgaag aggaaatcgc atcgtttcga
     9901 gatacactct cttccctatt tctgacgacc ggcccccgtg tggccgagtt tgagactctg
     9961 ttggcaacgt acttgggtgt ggctcattgc gtgggtgtga cgagctgcac acacggtttg
    10021 catctggcgc ttcttgccct cggcatcggt cctggcgatg aggtcatcac cacacctctt
    10081 acgtttgtgg cgaccgcgaa tgctatcgtg tacgcggggg caactcccgt gttcgccgat
    10141 gtcgatccag ccaccgggtt gttggatccg aaagcggtcg aggccgcgat cacatcgcgc
    10201 accaaagcgg tgatcgtcgt gcatctttac ggccaaatgg tcgatatgaa aacgtttcgc
    10261 gccctggcgg accgtcatca tctggctctc atcgaggatt cggcccatgg ggtcgagatg
    10321 cgacgcgatg ggattgcgcc cggacaacaa ggcgacgccg cggtgttcag tttttatgca
    10381 accaaaacga tgacctgtgg ggatggaggc gccgttgccg ttcgcgatac gaacctcgat
    10441 cgtcgcttac gacagctccg ctatcacggg atatcgaaag atgtgatggc gcgtcatgcg
    10501 accccttacg cgcattggga tatggtagaa ctcggctaca aggcggggtt gtcggatatc
    10561 gacgcggctc tgttgattcc gcagtttgga cgattggaac agcgccgtca taagcgagaa
    10621 ctggtggttc gcggatatcg tgatcggctc gaggcaacgc cagggttgtc gttcttgcgc
    10681 gtggagggaa tcagttcgca tcatttgtgt acggtattga ctccaaagga gcgtcgtgac
    10741 gatttgcttc gagaccttgg acaagcggga attggcgtag cggtcaatta ccgcgccatt
    10801 catgggctca cctattatcg agaacaacac ggttttcgaa agggacagtt tcctcacgcc
    10861 gaggcgatcg gagacagaac gatatcgctg cctctgtggc cgggattgcc cgaggatgac
    10921 attgattatg tagccgaacg actgcgtgac ttgatgcgtg tgggagggta agggccgtgg
    10981 gtgttggcat ggatgcgacc ggacacgccg tcggccatcc cgtcggagcc gatgggatcg
    11041 tctactccgt ggtcgttcct gtgttcaacg aggaagccaa tatcgaagca gtcgtgcgtc
    11101 gtgtgacgcc agtgctcgaa tccgaaggac aaccgtacga gatattgttt gtcgatgatg
    11161 ggagcaagga tcggactccg cagttgctcg cggcccaatg tcaaaccaac agccacgtgc
    11221 gggccattcg tttttcacgc aattttggcc aagaagcagc cgtgcaggcg gcgtatcttt
    11281 atgcgcgggg acagtggatt gtgcaaatgg atggggattt gcaaaatcct cctgaggaaa
    11341 ttccaaagct attgcgattg agagatgaag gctacgacat cgtttatggt gtgcgcacgg
    11401 gccgtcgcga tccttggttt cgaaaattcg cttcgcaaac gatgcgctgg agcatgcgtc
    11461 gcctgctgca tatcgagctg cccaaggata tctcgacgtt tcgtctgatg cgcgctgcga
    11521 cagccaaaac gttggcgcag tttccagagc ggcagaagtt tttatccgcc ctcgcctgtt
    11581 ggatgggagc caaacacacc acggtcgatg tggctcatgc ggctcgccag gccggacaca
    11641 ccaagtacaa tttgggcaaa ctcgtcaatc atacattcga tctggtcgtc gggttttcgt
    11701 cacgccctct tcggatgatt ggcgtgcttg gttttggcgc gggggtcatt ggatttggct
    11761 ttgccggttg ggctatcgtg gaaaagctc
//
LOCUS       DAOSOR010000113        11711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5458_length_11711_cov_4.135038, whole genome shotgun sequence.
ACCESSION   DAOSOR010000113 DAOSOR010000000
VERSION     DAOSOR010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11711
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5458_length_11711_cov_4.135038"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PK710_08280"
     CDS             <1..325
                     /locus_tag="PK710_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09753.1"
                     /translation="MLLYAVGGGRGGDVKLLAAVGALCRPVIGIEAVFYGFLVAAIYA
                     QARLAYEGKLLQTLGNTAFLAINPFLPKARRRKLTKESMTWTRLGPSVFVGTAIAVLL
                     NWSIP"
     gene            322..870
                     /locus_tag="PK710_08285"
     CDS             322..870
                     /locus_tag="PK710_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09754.1"
                     /translation="MIPSTLCRGKVSKPDRMVGKLLRDRRGAVYVEFLVAFLPLFIFF
                     ECLVQLSGLLTAKLVVQHAAVTATRAAAVVIYDDPQYYGDEKVGSVTGKRLEAIELAA
                     AIPMRSVRSLIDYELNFPSQPGGTDTRKDFGRDDLVRVRVKATYRCQVPIASRLVCDF
                     VSSTRNLTGEAALPNQGASYDY"
     gene            860..2365
                     /locus_tag="PK710_08290"
     CDS             860..2365
                     /locus_tag="PK710_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09755.1"
                     /translation="MITDKTTYQRVMGHAPSGWLADQRGAVMVVGVFMAAFLVGSLWY
                     IIGIGDAILYRERMQDGADAVAFAAAVYHARGMNIIAMINIIMAAILAVLVAFKLVQL
                     MNTIAMAISCALSWTGIGAAICSFTSGLAPLINNAVQIAEKLVNTTLPILSKTQVGIA
                     VAMPWVAQGKAVHVATTQYSEPVKGGGFVSVSLVPFVPKERLGLPVEEDEFSFLCKKA
                     GTYVGELVFSPFGKFGGWVAGVIGSIVETFPGYFCGDSGGGGGWSGGSGGMPQGLSQS
                     AIDKFAKEACEDKAKGKKNFDMDACIEDAKKDIEDELKQQNTSQSAMNGNGKTSKKVF
                     WGAKNGNGYFQIWSVLVGNDQWPKKAETGVKIAAWNRSMKMPDIPWGKVAFAQAEYYY
                     DEKGAWDDYKEDAMWNMFWRARLRRVHTPTSTFSSMIVDVVFDGILGKLDPLVAGALG
                     GTEVMGSLFGQSMGSTFEEIVGKVHEGGGPWQGQYIKNPNLANTAKIEIIH"
     gene            2377..2877
                     /locus_tag="PK710_08295"
     CDS             2377..2877
                     /locus_tag="PK710_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09756.1"
                     /translation="MMRRKLRKIRSSRGAAVVEAVVVIPFFIIIFASMIFVAKLYGEK
                     QRTLRVSKEYAWTYAMANCESGGANATLESEGSPMSQLESNDPNVNSDPLSQFQGSDP
                     GMEKFDKSWGTASSTIQGSATASKAIGGFTNHLSTTTKVQCNEKPQKGNLRGVFHFAW
                     SLFKQW"
     gene            2882..3841
                     /locus_tag="PK710_08300"
     CDS             2882..3841
                     /locus_tag="PK710_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09757.1"
                     /translation="MTDDLDLSKPSATTRLAEKARHRIHLRGVIRVLAYCFVVSVVLG
                     GLSIRSAWGNFKDSALIVGRQFASFGDLEGRIHRVRLNGEPVLVTSAVTTASMDDVLG
                     RFEALCRQDAGGLDKIFETLPANLKEEFETADGAAGVGIVRNQAGPEGMVACLSQQPL
                     EGWQSLPSRIEKFLSTGDLTHIGDLRYVYTKQMDGRTHVITVWTEGSFNLFNVAPMDG
                     QEAPGSDSPNAPRPEEAVRLLSATVEGAPYAVRIYDSAKPQQEVLAMYDSQMPSRGWS
                     PIPHATDDVAHGRAYTREGVDLLIFAFEQKDRSYVSVVEMSPR"
     gene            3947..4741
                     /gene="cpaB"
                     /locus_tag="PK710_08305"
     CDS             3947..4741
                     /gene="cpaB"
                     /locus_tag="PK710_08305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HOT09758.1"
                     /translation="MSKRALLLALIVATLGLVLMLLYVRRFEQQASGGERVQLLVAIK
                     PIERGAVITDDVLAVREVPLAYVEDRAVKAAERAKVVGLRLGNNVQSQQTLMWSDLAI
                     AADERRDLSALVQPGSRAVSVRARSGDKSYALIRPGDYVDVVAIMPEAQGDKRSAVVL
                     LQKVLVLAVGLDTANELMMDPNERNRNRTQEMVLTLSLHLQEAQLISLALEKGQITVA
                     LRNPEDQRIAERIPDMSSSALTDTKQREAVQNIRTGPVKLEAGDLR"
     gene            4738..6054
                     /locus_tag="PK710_08310"
     CDS             4738..6054
                     /locus_tag="PK710_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HOT09759.1"
                     /translation="MRTIVTLAALLSLVGVAGTSFAQKGDSGTLVETSEELSLAVGEN
                     KTISATEVSNYSEGTKGVADVKLTTDGKRFVIVGRAPGSTTLLLINKDGTQTKWVINV
                     FSRSPDAVEREVKQLLEGTSGVRVRRVGARFFIEGGVTAEADVRRIERIADLYPGQVE
                     SLVVVGAAPVERKVNIRIDFFFVQYERNSNYAFGMNWPTRLGGESIRSEVSYDFLSGT
                     VTTAQASIINQPLPGLDIAARNGWAKVLKQSTVITTNGTEAVFESGGEQNYPISAGLT
                     ASIQPITYGTNVTVLPRFDSDSGELEVKISADVSDLIPAIASTSLPGRSTSKLTTLVS
                     MKLGESLILSGIRTQSQRRSTAGVPLLSDIPILGLLFGSESQTREDVEGAVFIIPSVV
                     ESLPRDSLQLVNSAFAQYEEYSGDIEAINAYPKRPPMSGKRSGSKE"
     gene            6069..7958
                     /locus_tag="PK710_08315"
     CDS             6069..7958
                     /locus_tag="PK710_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOT09760.1"
                     /translation="MDIEVVIDSQGGRRIERISAVAPISVGRSPSCTVCLESDAVSRR
                     HAVFQVGPNSVVVEDVSTNGTIAGQQLLRRISAQVPFDTPVVVGEFTIRVRPVDYAAV
                     ARAPSAPTIEPQAAPLPAYMPPGVGMGASAPAYPTQGYVPPGVASSPTAKPPAPVPGV
                     ASRLVMRGSAPVQPSGPPIAKPRAKPAPPLNRLSAQSAIEAEAKRRAAADDVAMRREV
                     HKQLLEHLDLAAIDASKLDDPSMRPKVLTALRKIVGKLEDRLPAGTNQDRLVGELADE
                     ALGLGPLERYLADPTISEVMVVGPEAVYVEREGKLVKTDARFTDDERVRAVIERIVTP
                     LGRRIDESSPLVDARLKDGSRVNAVIRPIALRGSCITIRKFAKVPLTLPRLIELGALS
                     DSMGRFLQRCVVAKKNVVISGGTGSGKTTLLNVLSAAIPEDERIITIEDAAELQLEQP
                     HVVSLETRPANMEGKGEYTIRDLVRNALRMRPDRIVVGECRGGEALDMLQAMNTGHDG
                     SLTTTHANSPAEALARLETLALMAGVDLPSKAIRDQIASSIHVIVQQSRFSDGSRKVT
                     QIAEVAGLEDDGMIELLPIFEYERTGTGSAGQVMGRFRSTGYLPSFLDEFIVMGLIKS
                     GEPFL"
     gene            7955..8827
                     /locus_tag="PK710_08320"
     CDS             7955..8827
                     /locus_tag="PK710_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOT09761.1"
                     /translation="MNAFSGVNPEVFKWLGVFLIVVGVFIGTWSLAADPQSMVRRYWV
                     RYLAHLDRKLRSMFIWTPAKYIAWGQLAAAFLVIAGAVGIDLPLWYAWLVLVAVSPYY
                     YIERMRRKRVEMIEEQLDGFLVGLANALKAIPSISSAFVSVQAILRAPLRDEVELAIK
                     EMRVGSTLEQALLNMAGRVGSRQLDSALSAIVIGRQIGGNLPTILETTAGTLREMARL
                     EGVVKTKTAEGKAQLWVLAIFPFLMILALDMLNPGYFLPLEASVVGYIIAFFAGGFWL
                     ASLLVARKVLSVDI"
     gene            8824..9729
                     /locus_tag="PK710_08325"
     CDS             8824..9729
                     /locus_tag="PK710_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOT09762.1"
                     /translation="MNLSFEILRYLFVGMLTTALFIATYAVASAPSRVASRLGMRGLK
                     RQRAIADNPAWAQIEPLVRWLGVRVSGVLEDPLRGKMDRQLALAGDYLGLTPEEYLAF
                     SLLSTIGGAAFGAVAGYMLDMGFALTLATAPLGAALPYFAVSGEHTRRMKQINRGLPY
                     AADLLALAMSAGQDFPGAVRQVVEKSSDPDDALVEEFSRILQELQVGMTRRQALLAFS
                     ERAPLESVNEFVAAIVQAEERGNPVAEVLQIQAGVSRMRRSVKAEEAAAKAGVQMVGP
                     LFLLFFCIMLLVMGPMVLQLAAQAD"
     gene            9768..10640
                     /locus_tag="PK710_08330"
     CDS             9768..10640
                     /locus_tag="PK710_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09763.1"
                     /translation="MKALRFLVRYPDGRGEELTIEADRVLIGSGAHCEIRLPVDQAAM
                     EHVLVSMGPTSVMVEARAFEPPPTINGSPFQRAHLMPEAILGVGQVQMTVTIIEVLEK
                     QGLVQRKQEKTSPATYVLAVIAVPLSLYIIFSDSDGGQAEPPPTEVPKLWADPITSCP
                     QSSKQQALALAHDQNVLAIARRERRPFHIQDGVKAVPLFETAAACFEVAGENALAKEA
                     SNASQSLRREVGEDYRTHRVRLEHAISVGDWRTAQIETRVLLAFTDGLQGEYVTWLSN
                     LDRKLQLKYGRKNR"
     gene            10637..11227
                     /locus_tag="PK710_08335"
     CDS             10637..11227
                     /locus_tag="PK710_08335"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT09764.1"
                     /translation="MSPLPHRILVVVFSILFGGSGTACAPPPTPVVERNLEKVKQERE
                     PDKLVARGKAFAQMGDLTRAEQYFVSALEQGADADEVLPLLLRTCIESGRFRVAIDHA
                     RHGLKTHPNNSALRFLLGSLLQAVGEYREAIEQFERVVASNPEHADAHYALATIARDY
                     LGDLMRADIHFREYLRLNPKGPYADEARNSLLQSVP"
     gene            11224..>11711
                     /locus_tag="PK710_08340"
     CDS             11224..>11711
                     /locus_tag="PK710_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOT09765.1"
                     /translation="MIPREVYEETLLQFFAPIRPFLEDPSVSEVMINGPDQIFIERKG
                     KLYKTDATFDSKESLISVLRNAAQFVGKQIDESRPILEGRLPDGSRIEALIPPVAPEG
                     PFVSIRRFFQETLTVDRLIGFGSLTQDAAQTLEAMVIAKLNIVVAGGTGSGKTSMLNA
                     LTA"
BASE COUNT         2436 a         2820 c         3824 g         2631 t
ORIGIN      
        1 catgctcttg tacgcggtcg gcggtggcag agggggggat gtcaagctgc ttgccgccgt
       61 cggagcgctg tgccgtccag tgattggaat cgaagctgta ttttatggtt ttttggtagc
      121 agccatctac gcgcaagcgc gattggcgta tgaaggtaaa ttattacaaa cccttggaaa
      181 tactgccttt cttgccatca atcccttttt gccgaaagcc cgtcgtcgaa agctcaccaa
      241 agaatcgatg acgtggactc ggcttggccc gtcggtattt gtcggcacgg cgatagccgt
      301 attgttgaac tggagcatac catgatcccg agtaccttgt gccgcgggaa agtcagcaag
      361 ccggaccgga tggtaggcaa gctgctgcgg gaccgtcgtg gcgctgtcta cgtcgagttc
      421 ctcgtagcat ttttgccatt attcatcttt ttcgagtgtt tggtacagct cagtggtctg
      481 ctcacggcga agttggtggt gcagcatgcg gcggtgacgg cgacgcgggc ggcggcggtc
      541 gtcatttacg atgatcccca gtattatggg gacgagaagg tggggtcggt gacgggcaag
      601 cggttggagg ccatcgaatt ggcggcagcc attccgatgc gttcggtgcg cagcttgatt
      661 gattacgaat tgaatttccc gagccagccg ggaggaacgg atacgcgcaa agacttcggt
      721 cgtgatgact tggtgcgggt gcgggtcaaa gccacgtacc ggtgtcaagt tccgatagcg
      781 agtcgtctgg tgtgcgactt tgtatcgtcc acacgcaacc tgacgggcga agccgccctg
      841 cccaaccagg gagcgagcta tgattactga taaaacgaca taccaacgcg tcatggggca
      901 tgcgccatcg ggatggctag cggatcagcg cggtgcggtg atggtggtgg gtgtattcat
      961 ggcggctttc ctggtggggt cgctttggta catcattggc atcggggatg cgattttgta
     1021 tcgcgagcgg atgcaggatg gggccgacgc ggtagcgttt gcggcggcgg tttatcatgc
     1081 gcggggcatg aacatcatcg cgatgatcaa catcatcatg gctgccattt tggctgttct
     1141 ggtggcgttc aagctggtgc aactcatgaa cacgattgcg atggcgatct cctgtgcgtt
     1201 gtcgtggaca ggcatcgggg ccgcgatatg cagttttacc tcggggcttg cgccgctcat
     1261 caacaacgcc gtgcagattg ctgaaaagct ggtgaatacg accttgccca ttctcagcaa
     1321 gacgcaggtg ggcattgcgg tggcgatgcc ctgggttgcg cagggcaaag cggttcacgt
     1381 ggcgacgacg caatattcgg agccggtcaa ggggggtggg tttgtgagtg tatcgttggt
     1441 tcctttcgtt cccaaggagc gtctggggct gccggtcgaa gaagacgaat tctcattttt
     1501 gtgtaaaaaa gcgggcactt acgttggaga actcgtattc tcgccctttg gaaagttcgg
     1561 tggttgggtt gcgggggtga ttggcagcat cgtggagacg ttcccaggat atttttgcgg
     1621 ggacagtggc ggtgggggag gctggagcgg tggttcgggg ggaatgcctc aagggctttc
     1681 gcaatcggcg atcgacaaat ttgcgaagga ggcttgcgag gacaaggcta agggcaagaa
     1741 gaacttcgat atggatgcat gcatcgagga tgcgaaaaag gatatcgaag acgagttgaa
     1801 gcagcagaac accagtcaat ctgcgatgaa tggcaatggc aagacttcga agaaggtgtt
     1861 ttggggcgcg aagaacggca acggatattt tcagatctgg tcggtcttgg tgggcaacga
     1921 tcaatggccg aagaaggcgg agacgggggt gaagattgcg gcgtggaatc gctcgatgaa
     1981 gatgcctgac attccatggg ggaaagtggc gtttgcgcaa gcggagtatt attacgacga
     2041 gaagggggcg tgggatgact ataaggaaga tgcgatgtgg aacatgtttt ggcgcgcgcg
     2101 gctgcgtcgc gtgcatacgc ccacatcgac cttctcgagt atgatcgtgg acgtggtttt
     2161 cgatggcatc ctgggcaagc tggatccgtt ggtggctggg gcattagggg gcacggaagt
     2221 gatgggatcg ttgtttggcc agtcgatggg cagcaccttt gaagagattg tgggcaaggt
     2281 tcatgagggg ggtgggcctt ggcaggggca atacatcaag aatccgaatt tggcgaatac
     2341 ggccaaaatc gagatcattc attgaagcgg gaggcgatga tgaggcgcaa attacgcaag
     2401 atcagaagtt cccgcggggc cgcggtggtg gaagcggtcg ttgtcatccc gtttttcatc
     2461 atcattttcg cttcgatgat tttcgtggcg aagctatatg gggaaaagca gcgtacgtta
     2521 cgggtttcca aggaatatgc ctggacgtat gccatggcaa actgtgagtc tgggggggcg
     2581 aatgcgacgt tggaatcgga gggctcgccg atgagccagc tcgagtccaa cgacccgaat
     2641 gtgaattccg accctctcag ccagtttcag ggttcggatc cgggaatgga aaaattcgac
     2701 aaaagttggg gtacggcttc ttcgaccatt caagggtcgg ccactgccag caaggcgatt
     2761 ggtggtttta cgaatcatct atcgaccacg acgaaggtgc agtgcaacga aaagccgcaa
     2821 aagggcaatt tgaggggtgt gtttcatttc gcctggtctc tgttcaagca atggtgatgc
     2881 catgaccgac gacctcgatc tcagcaagcc ttctgcaaca acgcggctcg ccgaaaaagc
     2941 gcgacatcgc atccacctgc gtggggtgat tcgcgttttg gcctattgtt tcgtggtgtc
     3001 ggtggttcta ggcgggctat cgatccgatc ggcttggggc aacttcaagg attcggcatt
     3061 gattgtgggt cggcaattcg caagttttgg cgacttggaa ggcagaatcc accgtgtgcg
     3121 cctgaatggc gaaccggtct tggtgacgag cgcggtgacg acggcatcga tggatgacgt
     3181 attgggccga tttgaagcgc tatgtcgtca ggatgccggg ggattggaca agattttcga
     3241 aaccttgccg gcgaacttga aagaggagtt cgagacagcc gacggtgccg cgggcgtggg
     3301 gattgtgcgc aaccaggcgg ggccggaggg aatggtggcc tgcttgtcgc agcagccgtt
     3361 ggagggttgg cagagcttgc catcgcgcat cgaaaaattc ctaagtactg gagatctaac
     3421 gcatattgga gacttgcgct acgtctatac caagcaaatg gacggaagaa cgcatgtgat
     3481 tacggtatgg accgagggat ctttcaactt gttcaatgtg gcgccgatgg acgggcagga
     3541 ggcgccgggt tcggattcac ccaacgcacc acgtccggaa gaagcggtgc ggttgttgag
     3601 tgcgacggtg gagggggcgc cttacgcggt acggatttac gatagtgcga agccccagca
     3661 ggaggtgttg gcgatgtacg attcgcagat gccttctcgg ggctggagtc cgattccgca
     3721 tgcgaccgac gatgtggctc atggccgggc gtatacccgt gaaggggtgg acttgttgat
     3781 tttcgccttt gagcagaagg atcggtcgta tgtatcggtg gtggagatga gtccgcgctg
     3841 aaaacgactt cctgtccgtg ggtgccatcg tcgccaacga gatgacgccc ctcgctgtct
     3901 ctgttgttct ttcttctccc ttatcggact atactggccc tttctcatga gcaaacgagc
     3961 gcttttactc gccctcatcg tcgcaaccct cggccttgtg ttgatgctgt tgtacgtcag
     4021 gcgcttcgaa cagcaggctt ctggcggtga acgtgtgcaa cttctcgtcg ccatcaaacc
     4081 gattgagcgc ggggcggtca ttaccgatga cgtattggcg gtgcgggagg ttccgctcgc
     4141 ttatgtcgag gaccgtgcgg tgaaagcggc ggagcgggcc aaggtggtgg gattgcgtct
     4201 tggcaacaac gtgcaatcgc agcagacgct gatgtggtcg gaccttgcga tagcggccga
     4261 tgagcgaaga gatttatcgg cgttggtgca gccggggagc cgagccgtat cggtgcgagc
     4321 gcgcagcgga gacaagagtt acgcgttgat tcggcccggt gattatgtcg atgtggtggc
     4381 gatcatgcct gaggcgcaag gcgataagcg cagcgccgtg gtgttgttac agaaggtttt
     4441 ggtgctcgcg gtggggttgg ataccgccaa tgagctgatg atggatccga atgagcgcaa
     4501 tcgaaatcgt acgcaggaga tggtgcttac gttgagtttg catttgcagg aggcgcaact
     4561 catctccctg gcgcttgaaa agggccagat caccgtagcg ttgcgcaatc cggaggacca
     4621 gcgcattgca gaacggatcc cggatatgtc atcgtcggca ctgacggaca ccaagcagcg
     4681 agaagcggta caaaacattc gaactgggcc cgtcaagctc gaagcaggag acttgcggtg
     4741 agaacaatcg tcacactggc agcgttattg tcgctcgttg gcgttgcggg tacgtctttc
     4801 gcacagaaag gcgattcggg aacgttggtc gagacttcgg aggaattgag tctcgcggta
     4861 ggggagaaca agaccatttc cgcgaccgag gtgagcaatt attcggaggg gaccaagggc
     4921 gtagcggacg tcaagttgac tacggatggc aagcggtttg tgatcgtggg ccgtgcgccg
     4981 gggtcgacga ccttgttgtt gatcaacaag gatggcacgc agaccaagtg ggtcatcaat
     5041 gtgttttcgc gttcaccgga tgcggtagag cgtgaggtga agcaattgtt ggagggcacc
     5101 tcgggggtgc gggttcgtcg agtgggagcg cgatttttca tcgagggagg ggtaacggcg
     5161 gaagccgatg ttcggcgtat cgagcgcatt gcggatttgt atcctgggca ggtcgagtcc
     5221 ttggtggtgg tgggggcggc accagtcgag cgaaaagtca atatccgcat cgattttttc
     5281 ttcgtgcagt acgagcgcaa ctccaattat gccttcggaa tgaattggcc gactcggctt
     5341 ggcggggaat cgatccgcag cgaggtaagt tatgatttct tgtcaggaac ggtaacgacc
     5401 gcgcaggctt ccatcatcaa tcagccgtta ccgggtctgg atattgccgc gcgcaatggg
     5461 tgggcaaagg tcctcaagca atccacggtg attacgacca acggaaccga agcggttttc
     5521 gaaagtggag gcgagcagaa ctacccgatt tctgccgggc tgaccgcgag cattcagccc
     5581 atcacgtatg gcaccaacgt gacggttttg cctcgcttcg attccgacag cggggaacta
     5641 gaggtgaaga tatccgccga cgtaagtgac ctcattcccg ccattgcatc gaccagtctg
     5701 cctggtcgaa gcacgtccaa actcacgacc ttggtgagca tgaaactcgg tgaatcacta
     5761 attttatcgg gtatccgaac gcaatcccag cgtcgttcca ccgcgggcgt accgctgctg
     5821 agcgacatac cgattttggg cttgttgttt ggaagtgaat cacaaacccg agaggatgtg
     5881 gagggagccg tattcatcat tccttcggta gtggagtcct taccccgcga ctctttgcag
     5941 ctcgtcaaca gcgctttcgc acaatatgaa gagtattccg gcgatatcga agccatcaac
     6001 gcgtacccca agcgtccacc gatgtcgggc aaacgctctg gcagcaagga gtagtggaat
     6061 cgggcagcat ggacatcgaa gtcgtcatcg actcccaggg tggtcgccga atagaacgga
     6121 tatcggcggt ggcgcccatt tccgtcggca gaagcccctc ttgtacggtg tgtctcgaga
     6181 gcgatgcggt atcacgacgg catgcggtgt ttcaggtagg acccaattct gtcgtggtcg
     6241 aggatgtatc gacgaacgga acgatcgctg gtcagcagtt gttgcgtcgg atctcggcac
     6301 aagtgccttt cgatacgccc gtggtggtgg gtgagtttac gatacgggtt cgtcctgtgg
     6361 attacgcggc ggtcgccagg gcaccgtcgg caccgacgat tgagccgcaa gcggccccac
     6421 tgccagcgta tatgcctccg ggcgtgggca tgggagcatc ggcgccggcc taccctacgc
     6481 aaggatatgt ccctcctggg gtagcgtcat cgccgacggc caagccacca gctcctgttc
     6541 cgggcgttgc ttctcggctg gtcatgcgag gcagcgcacc ggttcaacct tccggtccgc
     6601 ctatcgccaa gcctcgtgca aagcccgcgc ctcccttgaa tcgactatcg gcccaatcgg
     6661 cgatcgaagc ggaagccaag cgcagagcag ccgccgacga tgtggcgatg cgtcgagaag
     6721 tccacaaaca gcttttagag cacttggact tggcggcgat cgacgcctcc aagctcgatg
     6781 atccttcgat gcgtccgaag gtattgacgg cgttgcgcaa gatcgtaggc aagctggaag
     6841 acaggcttcc tgcgggaacg aatcaggatc ggttggtggg ggaattggcg gatgaggcgc
     6901 taggtcttgg ccctttggag aggtatttgg cggacccgac catcagtgaa gtgatggtgg
     6961 tggggcctga agcggtctat gtggaacgag aggggaaatt ggtaaagacc gacgcgcggt
     7021 ttacggacga cgagcgggtg cgagcggtca tcgagcgtat cgtaaccccg ttgggtcgtc
     7081 gcatcgacga atccagtccg ttggtggatg cgcgattgaa agacggttct cgtgtcaacg
     7141 cggtgattcg tccgattgcg ttacgtggtt cgtgcatcac gatccgcaag tttgccaaag
     7201 taccgcttac gttgccgcga ttgatcgaat tgggcgcgct atcggattcg atgggcaggt
     7261 ttcttcagcg ttgtgtggtg gcgaaaaaaa acgtggtgat atcgggggga acgggcagtg
     7321 gaaagacgac actattgaat gtgttgtcgg cggcgattcc ggaagatgag cgaatcatta
     7381 cgatcgagga tgcggcggaa ctccagctcg agcagcctca cgtggtatcg ttggagacgc
     7441 gtcctgcgaa catggaaggc aagggggagt acacgatacg ggacttggtg aggaatgcgc
     7501 ttcgtatgcg tcccgatcgc attgtggttg gggaatgccg cgggggtgag gcattggaca
     7561 tgctccaggc gatgaatacg ggtcatgatg gctcgttgac gacgacgcac gccaacagtc
     7621 ccgccgaagc gctggcgcgt ctcgagacct tggcgttgat ggcgggagtg gatctgccat
     7681 cgaaagcgat aagggatcag attgcatcga gcattcatgt gatcgtgcag cagtctcggt
     7741 tcagcgatgg gtcacgcaag gtgacgcaga tcgcggaagt ggccgggctg gaggatgatg
     7801 ggatgatcga gcttttgccg attttcgagt atgaaaggac ggggacgggt tcggcaggtc
     7861 aggtgatggg cagattccgt tcgacaggat atttgccttc gtttttggat gaattcattg
     7921 tcatggggtt gatcaaatcg ggggagcctt tcttatgaat gcattttcgg gcgtgaatcc
     7981 ggaggtattc aagtggttgg gtgtgttcct gatcgtggtg ggggtattta ttggtacgtg
     8041 gtcgttggcg gccgatcctc agagcatggt gcgacggtac tgggttcggt atttggcgca
     8101 cttggaccgt aaacttcgtt cgatgttcat ttggaccccc gcgaaatata ttgcgtgggg
     8161 gcagcttgcg gcggcgtttt tggtgattgc gggggcggta gggatcgact tgcctctttg
     8221 gtatgcgtgg ttggttctgg ttgcggtatc gccctattat tacatcgaga ggatgcgtcg
     8281 aaagcgcgtg gagatgatcg aggaacagct cgatggtttc ctcgtggggt tagcgaatgc
     8341 attgaaagca attccgagta tttcgagcgc gttcgtatcg gttcaggcga tccttcgtgc
     8401 gcccttgcgt gatgaggtgg agttggcgat caaggagatg cgtgtgggaa gcacgttgga
     8461 acaagcgtta ttgaacatgg cggggcgtgt aggaagtcga caactggatt ccgccttgtc
     8521 tgcgattgtc attggacgtc agatcggagg gaacttgccg acgattttgg agacgactgc
     8581 gggaaccctg cgagagatgg cgcgtttgga aggggtggtg aagaccaaga cggcggaggg
     8641 caaagcgcaa ctatgggtgt tggccatctt tccctttctg atgatactgg ccttggacat
     8701 gttgaatccc ggttattttc tgcctttgga agcaagtgtg gtgggatata tcatcgcgtt
     8761 tttcgcgggt gggttttggt tggcgtcgct gttggtggcg cgcaaggtcc tttcggtgga
     8821 tatatgaacc tgagtttcga gatcttgcgc taccttttcg tcggcatgct gacgacggcg
     8881 ttgttcattg cgacgtacgc ggtggcctcc gcgccatctc gggtggcaag tcggttgggg
     8941 atgcgcgggc tcaagcgtca acgagcgatc gcggacaatc cggcgtgggc gcagatcgag
     9001 ccgttggtac gttggcttgg ggtacgggtg agtggtgtgt tggaagatcc gcttcggggc
     9061 aagatggaca gacagcttgc gctcgcgggc gactacttgg gattgacgcc ggaggagtat
     9121 ctggcattca gcctcctttc gacaatcgga ggagcggctt ttggagcggt agcggggtac
     9181 atgctggaca tggggtttgc gttgacgcta gcaacggcgc ccttgggagc ggcattgcct
     9241 tactttgccg taagcggcga gcatacccgt cggatgaagc agatcaaccg ggggcttccg
     9301 tatgctgcag acttgttggc tttggccatg agtgctgggc aggacttccc tggggcggtt
     9361 cggcaggtcg tggaaaagtc aagcgacccg gacgatgccc tggtcgagga attctcacgt
     9421 attttacagg agttacaagt ggggatgacc cgtcgacagg cgctgctggc cttctccgaa
     9481 agagcgcctt tggagtccgt caacgagttt gtggccgcga tcgttcaagc cgaggagcga
     9541 ggcaatcccg tcgcggaagt attgcaaatt caagccggag tttcgcggat gcgtcgcagc
     9601 gtcaaagcgg aggaagcggc ggcaaaagcg ggcgttcaga tggttggccc tttgtttctg
     9661 ttgtttttct gtatcatgtt gttggtgatg ggaccgatgg tccttcagct tgcggcgcaa
     9721 gccgattgac agccgaatga cagccgaatg acaacgagga gacgtgcgtg aaggctctca
     9781 ggtttctggt tcgctaccct gacggacgcg gagaggaatt gacgatcgag gccgaccggg
     9841 tgctgatcgg gagcggtgcg cactgtgaaa tccgtcttcc ggtcgaccaa gcggcgatgg
     9901 agcatgtcct ggtcagcatg ggccccactt cggtcatggt cgaagcgcga gcgttcgaac
     9961 caccgcccac catcaacggc tcgccttttc agcgagcgca tttgatgccg gaagccatcc
    10021 ttggtgtcgg acaggtccag atgacggtca ccatcatcga agtcctagaa aaacaagggt
    10081 tggtgcagcg aaaacaggaa aaaaccagcc ctgccaccta cgttctcgcc gtgatcgccg
    10141 tccccttgtc gctgtacatc attttctcgg attcggatgg cggccaagcc gaaccaccgc
    10201 ccacggaagt ccccaagtta tgggccgatc caatcacctc ctgcccacaa tcctcaaaac
    10261 aacaggcctt ggcgcttgcc catgaccaaa atgtgctcgc catcgcacgg agagaacgtc
    10321 gccccttcca catccaagat ggagtcaagg ccgtaccctt attcgaaacc gccgccgctt
    10381 gtttcgaagt ggctggagaa aacgccttgg ccaaggaagc ctccaacgct agccaatccc
    10441 tgcgtcgcga agtgggtgaa gactaccgta cccacagagt tcggctcgaa catgcgatat
    10501 cggtcggcga ttggagaaca gcacaaatcg aaacacgcgt tttgctcgct ttcaccgacg
    10561 gtctccaagg ggaatacgtc acctggttat cgaacctcga ccgcaaacta cagctcaagt
    10621 acgggcgaaa gaaccgatga gccccctccc ccatcggatc ttagtagttg ttttttcaat
    10681 actttttggt ggttcgggaa ccgcttgcgc ccctcctcct acccctgtgg tcgagcgcaa
    10741 cctcgaaaag gtcaaacagg agcgtgagcc ggacaagctt gtggcgaggg gcaaagcgtt
    10801 tgcgcagatg ggagacctga cgcgggcgga gcaatacttc gtgtccgcgt tggaacaggg
    10861 cgcggacgcg gacgaggttt tgcctttgct gctccgaacg tgcatcgaat caggtcgatt
    10921 tcgtgtcgcc atcgaccatg ctcgccacgg gttgaagact catccgaaca attcggcatt
    10981 gcgctttttg ctcggctcgc tactgcaagc ggtgggtgaa taccgtgaag ccatcgaaca
    11041 gttcgaacgg gtcgtcgcca gcaatcccga gcacgccgat gcgcattatg cgttggcaac
    11101 catagcacga gactatttgg gagatctcat gcgtgcggac atacacttcc gcgaatactt
    11161 gcgcttgaac cccaagggcc cctatgccga tgaggcaaga aattccttgc tccagagtgt
    11221 gccgtgatac cacgagaagt ctacgaagaa accctgttgc agtttttcgc gcccatccgt
    11281 ccgtttctgg aggatccgtc ggtcagcgag gtgatgatca atggaccgga tcagatcttc
    11341 atcgagcgaa aaggcaaact atacaaaacc gatgccacgt tcgactccaa agaatcactc
    11401 atctccgtat tgcgaaacgc agcgcaattc gtaggaaagc aaatcgacga aagcagaccc
    11461 attctggaag gtcgtttgcc cgatggaagt cgtatcgaag cgctcattcc gccagtggct
    11521 cccgaaggac ctttcgtttc cattcgccgg ttctttcaag aaacgttgac ggtggaccga
    11581 ttgattgggt ttggatcgtt gacgcaagac gctgcccaaa cgcttgaagc gatggtgatt
    11641 gcgaagttga acatcgtggt ggccggaggc acgggaagtg gaaaaacatc gatgctcaac
    11701 gcgctcacgg c
//
LOCUS       DAOSOR010000114        11704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5463_length_11704_cov_4.666924, whole genome shotgun sequence.
ACCESSION   DAOSOR010000114 DAOSOR010000000
VERSION     DAOSOR010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11704
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5463_length_11704_cov_4.666924"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..102
                     /locus_tag="PK710_08345"
     tRNA            14..102
                     /locus_tag="PK710_08345"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48..50,aa:Leu,seq:taa)
     gene            complement(254..1825)
                     /gene="hutH"
                     /locus_tag="PK710_08350"
     CDS             complement(254..1825)
                     /gene="hutH"
                     /locus_tag="PK710_08350"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOT09766.1"
                     /translation="MYTPIAKEQSPVLLGQPISLQDLDDVARRGRPVALCSSAREKMA
                     RSRAAIDRIVTGGNESPNVYGVNTGFGALSETRIDAHDIRALQRNLVRSHACAVGPEL
                     DVPWVRAMTMLRAQVLALGHSGVRVQVVELLMGMLNRDVHARIPSQGSVGASGDLAPL
                     AHLALVLQGEGEARVEGGLLPGAEALRLAGLEPIELQAKEGLALINGTQMMAALGAIA
                     LLESAMLVRYADLAGAMSLEALQGSKKPFDSRIMDVRSHPGQAIVAANLRTLLADSQI
                     MESHAHCHKVQDPYSLRCMPQVHGASRDALAWANEVIGRELNAVTDNPLVFLRDDGEA
                     ELISGGNFHGQPLALALDLAALAVAELANISERRVEQLVNPALSSGLTPFLAAHSGLD
                     SGLMIAQVASASLVSENKVLSHPASVDSIPSSAGKEDHVSMGSVSARKLMQVVDNVWV
                     CLAIEVLGASFGLDQRQPLRAGKGVYAAHQALREVVEPLTADRVLYQDIQRVQAFMRS
                     SELLNRVQTTVGPMG"
     gene            complement(2024..2650)
                     /gene="udk"
                     /locus_tag="PK710_08355"
     CDS             complement(2024..2650)
                     /gene="udk"
                     /locus_tag="PK710_08355"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF004018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOT09767.1"
                     /translation="MTTLMIGVSGGTGSGKTTIARALLDGLPRTRAVLLQHDSYYRDH
                     PELSYEERSGLNYDHPSALDTELLVTHLDALRKGKKVSVPVYDFASHRRKEHTTLVEP
                     APVIIVEGILVLAEKDVRDRLDIKLFVDTAADIRLMRRVRRDLEERGRTFAQVRKQYY
                     ESVRPMHQAFVEPSKAYADIIIPEGGKNLVAVDMVVARVRQWLQQFGV"
     gene            2701..3210
                     /locus_tag="PK710_08360"
     CDS             2701..3210
                     /locus_tag="PK710_08360"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOT09768.1"
                     /translation="MSVPKISVYSIKSLFRALSHPPSLRAKRLGPNLREKIILRVSSI
                     NQCNVCSVFHENLARWEGLSRQDIAEARNPDSDDEQDGDTRLLFHYAEIRTSNLEKEF
                     PEVVQAFEQAFDEATQREVRAIVDLFTFNNRFNNSWEGWLPGAKRRRQSMGLTCPRVD
                     HTQRTSAKP"
     gene            complement(3194..4018)
                     /locus_tag="PK710_08365"
     CDS             complement(3194..4018)
                     /locus_tag="PK710_08365"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOT09769.1"
                     /translation="MSDKADHDLGRDVMGEVSRRAALHGVIALGGLWFLPMAGCERPL
                     VASVPQPDPNPHPSSSSPMEKEPAMPTEYTLPPLSYAYDALEPHIDAQTLRLHHDMHH
                     AGYVKGANAALANLEQARKSGDYNLVDLYSKKLAFHLSGHLLHSLFWENMAPVGKGGE
                     PSKPLDAAIRAAFGSLEAMKAQMSAVAVSVEGSGWAVLGYHPIDQQLSLLQCENHEKK
                     AVWGTIPLLVVDVWEHAYYLKYQNKRADFVAAWWNLVHWNDVSARYAKAKTLTALR"
     gene            4251..4946
                     /locus_tag="PK710_08370"
     CDS             4251..4946
                     /locus_tag="PK710_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09770.1"
                     /translation="MGCQNNKPSCIQECEKAFESQEKACGSKTQSEADSCSQKAKESR
                     WSCRKQCGLDEPSPTPLASPAATPTASSPAIPSVLDLGKVCDGKAEPTAAAVDNTSGA
                     HNAAVVATQNLGSDLYLVDTITNSYDSIGPKSGKSKQADAYALVVCVSVKKHQKVKEC
                     PFAKHTLELHDATYQIRVVEARTANELKSIDIAVAADRQCPPTSTSHQQRDISLPSFV
                     KTVLEEADLHVHP"
     gene            complement(4994..7078)
                     /gene="uvrB"
                     /locus_tag="PK710_08375"
     CDS             complement(4994..7078)
                     /gene="uvrB"
                     /locus_tag="PK710_08375"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOT09771.1"
                     /translation="MPRFELVSSFLPKGDQPKAIEEICDRLRRHERFTTLLGITGSGK
                     TFTIANVIATVNRPALIMAPNKTLAAQLYSEMRDLFPQNAVEYFVSYYDYYQPEAYIP
                     VTDTFIEKDSIINDHIDRMRHAATAALLSRHDVIIVASVSCIYGIGSAESYAGLLVPI
                     ETGVELRRDELLRKLVDIQYERNDIDFHRGTFRVRGDVVEVFPAYEQDRAVRVEFFGD
                     EVEAIRQIDPMRGRVLQTLERYDVYPGSHYVTPEAQLRKAAESIRAELLERSQFFDGQ
                     GRLLEKQRIEQRTLYDLEMLEQMGYCQGIENYSRHLSGRKAGDAPPTLIDYFPDDFLL
                     IIDESHQTLPQIKAMYQGDRSRKSTLVDYGFRLPSALDNRPLRYDEFESRIKQCIFVS
                     ATPGEEEIGLTAGEVVEQVIRPTGLTDPRVEVRTVTGQVDDLLGEIRVRVQAGERVLV
                     TTLTKRMAEDLTDYYQELGIRVRYLHSDVETLERIELLRDLRLGEFDVLVGINLLREG
                     LDLPEVSLVAILDADKEGFLRSARSLIQTIGRAARNVNGRVIMYADRITDAMNRALEE
                     TNRRRERQTRYNETHGITPETVKKAIADISPSSGNTDYYTIPSRRRESSAAAETEISL
                     QERIEAMRAEMFLAAEALDFERAARLRDTLKSLRALEEPQTSAIAANPVKKSRKSSLR
                     DAPKSARPRRRR"
     gene            complement(7124..7288)
                     /locus_tag="PK710_08380"
     CDS             complement(7124..7288)
                     /locus_tag="PK710_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09772.1"
                     /translation="MSRVLWLLVGALLLAACGDDERETPNCEDMSKCVTEPSGGTPIS
                     SDAGSDSASD"
     gene            complement(7288..7902)
                     /locus_tag="PK710_08385"
     CDS             complement(7288..7902)
                     /locus_tag="PK710_08385"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HOT09773.1"
                     /translation="MTPSSPPRPSIPSTLPKKSSGAPYIAAIIILVLLIIGLVWFKFS
                     RKDPPVQTPVIQSMTVETAQPPPILDIPAPPEIEAGPDSGPDSGKKNTATSGTNLCSS
                     PCNGNAPPALRSALSSTAGAARGCYERALRTNPMLEGRMMVAVRVASNGNVCSASVVQ
                     DSVGSAQVTSCVSSLFSGKRFPPVTDGRCADVQVPLSFKPRENK"
     gene            complement(7943..8533)
                     /gene="rsmD"
                     /locus_tag="PK710_08390"
     CDS             complement(7943..8533)
                     /gene="rsmD"
                     /locus_tag="PK710_08390"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOT09774.1"
                     /translation="MRIVAGVLRGRRLRVPTGKTTRPTTERVREALFDMLGSSIQQRN
                     VLDLYAGSGALGIEALSRGARRTVFVEAAPSAAQIIRANLVNLALTDPSVAQVVVRAV
                     ERSFSVLRAFSPFDLCFVDPPFSAVRDRSALRAVEAMVEEGILSPHAELIFESPSDQP
                     VPSLQGFGLDDFRQYGDTHLAFFSLANGKNKLQRET"
     gene            complement(8602..9741)
                     /locus_tag="PK710_08395"
     CDS             complement(8602..9741)
                     /locus_tag="PK710_08395"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOT09775.1"
                     /translation="MRRGLMLLGPFLILASPSVGFADPPSPDTPAITVVRIKTEDSDA
                     QADALTTALQAEVRNLAGWSLAEGDYSLEVLTMALRCPWPPDAACEARIAQEIKADRY
                     IWGTIDEKSGKAVGTLHLWQRDQGQTKVDLSYASNLTEANDETLRQIVREALATLTGG
                     PPKGSVDIRAGDVNGQVLVDGKPVGTIQNGKATIFVPAGTRRIEVRAPGYAPVSGDVT
                     VHPNGSVSLTLHPVAQENADSSEGPNYRRIGAYSALVAGGALAIGGVYSSLQVNSINN
                     DNAFIAYREGVEGDVCEAAKNGVYSGKAGAASPQTVEDKCAASDKFSVLQWVFYGLSA
                     VSVGAGTYLLVTEKPDEKGPSTANQLQVLPTAVRGGGGLDLRLKF"
     gene            complement(9796..10407)
                     /locus_tag="PK710_08400"
     CDS             complement(9796..10407)
                     /locus_tag="PK710_08400"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT09776.1"
                     /translation="MQHGWPLWAALGLMMSVTSISLADDTKAQTQDSKAATTKRRDPK
                     GITGISPFMELIVKGSSAYVARDFPTAISTYQEAIQKTPNRPLGHYMLGQAFLADKKI
                     DQAEGAWQTALRYANSDPVVRAKILFVLADLREQQGRWDDAIKAWKAYADFIASEPKA
                     KGYPATPTERIRIIEKRQELEAKYAKVKERIAQREQDVAEKAK"
     gene            complement(10591..>11704)
                     /locus_tag="PK710_08405"
     CDS             complement(10591..>11704)
                     /locus_tag="PK710_08405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOT09777.1"
                     /translation="DATGASLHGIVEVLEYERRRGSPERSRLALFDASGKVMFIDGPN
                     PDDPGTVAVRAPIHTRHGVAGHVQVVKETVIMRRLLADFAPTVLVISAILGAAAALAA
                     ALIGRAIAAPIESLTHFAERVSEGDRAVTPPVPHGLEVKRLTKAIDKMRRQLDGLPYV
                     ESFAADLSHELKNPVAAIRASAEVLDDGALAEPEEAARFVKRIREATGRIEVILSELL
                     SLARIESRGIENFDSVDLQAVVRSILESHATRGKVLFEPGGPVRIRGDGRWLARAIDN
                     LVHNAFSHGDGSQPPRISLHTLGPNAMVTVSNPGTLLPHVRKRLFRRFVTSRGDKGGT
                     GLGLPIVRAVAEAHRGKVEVECDDTTVTFRLVLPKE"
BASE COUNT         2538 a         3389 c         3286 g         2491 t
ORIGIN      
        1 cgcttgtctt ttcggccccc tggcgaaatt ggcatacgcg gcgggcttaa aacccgctgc
       61 cgttcacacg gcgtaccggt tcgaacccgg tgggggctac cattttttta tttttttctc
      121 cccacacccc gcaacgcgtc gatgagatgc gtcatctcac ctaaccgtcc gggacctcct
      181 ccaacaatcc acctccgggc cctggctgcg gccaagtttg gctagatcaa acttggccgt
      241 cgcctttttg tcttcacccc atgggcccca ccgtcgtttg cacgcgattc agcaactccg
      301 aggaacgcat gaaagcctgg acccgttgga tgtcctggta caagacacgg tccgcggtca
      361 gcggttccac cacctcacga agcgcttgat gggcggcgta gacaccttta ccagcccgaa
      421 ggggttggcg ctggtcgaga ccaaacgagg ccccaagcac ctcgatcgcc aaacataccc
      481 atacgttgtc cacgacttgc atcagcttgc gcgccgatac ggaacccatc gatacatggt
      541 cttctttccc cgcactcgat ggaatcgaat cgacactcgc aggatgtgac aacactttgt
      601 tttcactcac gagcgaagcg cttgctacct gggcgatcat caaaccgcta tccaatccac
      661 tatgtgcggc aagaaatggc gtaagcccgc tggatagggc cggattgacg agttgctcca
      721 cacgacgttc gctgatattc gccagttcgg cgaccgccag ggcagccaag tccagcgcca
      781 acgcgagcgg ttgcccgtgg aagttgcctc ccgaaatcag ctccgcctcc ccatcgtcgc
      841 gcaaaaacac cagcggattg tccgtcaccg cattgagttc acgcccaatc acttcgttgg
      901 cccaggccaa cgcatcccga gacgcaccat gcacttgcgg catacatcgc aaggaatacg
      961 ggtcctgcac cttgtggcaa tgcgcgtggc tttccatgat ctggctgtcg gcgagcaagg
     1021 tgcgaaggtt ggcggcgacg atcgcctgcc ccgggtggga acgaacgtcc atgatacgag
     1081 aatcaaaggg ttttttggaa ccttggagcg cttctaggct catggcgccg gccagatccg
     1141 catagcggac cagcatggcg gattcgagca acgcgatggc tcccaaagcc gccatcatct
     1201 gcgttccatt gatgagggcc agcccttctt tggcttgcag ttcgatcggc tcgagcccgg
     1261 ccaaccgaag cgcttccgca ccaggaagca atcccccctc tacccgcgct tccccttccc
     1321 cctgtaatac caacgccaaa tgcgccaaag gcgctaggtc ccccgaggcc cccacggaac
     1381 cttgcgatgg tatccgcgca tgaacgtcgc gattgagcat ccccatgagc aactccacca
     1441 cctgcacgcg taccccggaa tgccccaacg ccaacacttg cgcgcgcaac atggtcatgg
     1501 cgcgaaccca tggcacatcg agttccggtc ctaccgcgca cgcgtgactt cgcaccaagt
     1561 tgcgttgcaa agcccgaata tcatgcgcat cgatccgggt ttcactcagc gctccaaacc
     1621 ccgtgttgac cccataaacg ttgggagatt catttcctcc cgtcacgatc cgatcgatgg
     1681 ccgcccggga ccgagccatc ttttcccgcg ccgaagaaca taaggccacg ggtcttccac
     1741 gacgcgccac atcatcaaga tcctgcaggc tgatgggttg tcccagcaat acgggagact
     1801 gctctttcgc aatgggtgtg tacatgattt gtcctcatac cgacaagggt cgctccttcg
     1861 tgtagcgctc ggctctcgtc gcgtctacgg ccacgaaaca cagcaacggc cttccgctca
     1921 tcgaaggcag gccaccagca caacccttcc tcccctcacc gcggctgaca ccgctaggca
     1981 ccgaacgttc gtgcgcgttc gtgcgtgaac gccgcccagc acgttatacg ccgaattgct
     2041 gcaaccactg acgtactcgc gccaccacca tgtctacagc caccaggttt ttccctcctt
     2101 cgggaatgat gatgtccgca taagccttgg atggctccac aaaagcttga tgcatcggcc
     2161 gtaccgattc gtaatactgc ttgcgaacct gggcaaacgt cctgcctcgc tcctcgaggt
     2221 cccgtcgaac ccgccgcatc agccgaatat ccgccgccgt gtccacaaat agcttgatat
     2281 ccaaccgatc ccgaacatct ttttccgcga gcaccaagat cccttccacg atgatcacag
     2341 gcgccggctc gacgagagtc gtgtgttcct tgcgacgatg ggaggcgaag tcatagaccg
     2401 ggacactcac cttttttcct tttcgcaaag cgtccaggtg cgtgaccaga agttccgtgt
     2461 cgagcgccga ggggtggtcg taattcaatc cggaacgttc ttcgtaggaa agctccggat
     2521 gatcccgata gtagctgtca tgctgaagaa ggacagctct cgttcgcgga agcccatcga
     2581 gcaacgcacg agcgatggtg gtttttcccg aacccgttcc acctgatacg ccaatcatca
     2641 aggttgtcac gatatgcgtt tctagcaggt tctttgaaaa aaggggggta caatcagcgc
     2701 atgtcggtgc ccaaaatatc ggtttattct ataaaaagcc tatttcgtgc tctttcccat
     2761 cccccttcgc tgcgagccaa acggctcggc ccaaaccttc gtgaaaaaat cattcttcga
     2821 gtctcgtcca tcaaccaatg caacgtatgc tccgtttttc acgaaaacct tgcccgctgg
     2881 gaaggcctga gccgccagga catcgccgaa gcccgcaatc ctgactccga cgacgaacag
     2941 gatggggata cccgcttgct gtttcactac gcggagattc gcacctcgaa tctcgaaaag
     3001 gaatttcccg aggtcgttca ggcgttcgaa caagctttcg atgaagccac ccagcgagaa
     3061 gtccgcgcca tcgtcgacct tttcaccttc aacaaccgat tcaacaattc gtgggaagga
     3121 tggctacccg gcgccaaacg acgccgtcaa tcgatggggc tcacgtgccc gcgcgtcgat
     3181 cacacccaac gtactagcgc aaagccgtga gcgttttggc tttcgcatag cgcgcggata
     3241 cgtcattcca atggaccagg ttccaccacg ccgccacaaa atccgcccgt ttgttttggt
     3301 acttcaagta atacgcatgc tcccacacgt cgacgaccag caaggggatg gttccccaca
     3361 ccgctttttt ctcatggttt tcacattgaa gcagagaaag ctgttggtcg atcggatgat
     3421 accccaatac ggcccagcca cttccctcca ccgaaacggc taccgcggac atttgcgctt
     3481 tcatggcctc caggctccca aaagccgcac gaatcgccgc gtccaaaggc ttggaaggtt
     3541 cccccccttt gccgactggc gccatattct cccaaaatag agaatgaaga agatggccgg
     3601 acagatgaaa agccagcttt ttcgagtata aatcgaccag gttgtagtct ccgctttttc
     3661 ttgcttgctc gagattcgcg agcgccgcat tcgctccctt gacatatccc gcatgatgca
     3721 tgtcgtgatg aagccgcaac gtctgcgcat cgatatgagg ctccaaggcg tcgtaggcat
     3781 aggagagagg cggaagagtg tattccgtag gcatggcagg ctccttttcc atcggtgaag
     3841 aagacgaggg atgaggattg gggtcaggtt ggggcaccga agccactagc ggtcgttcac
     3901 atcccgccat cggcaaaaac cacaatcccc ctagcgcaat cacgccgtga agggccgctc
     3961 gtcgtgatac ctcccccatg acgtctcgcc ccaggtcgtg gtctgctttg tcgctcatga
     4021 cgatatcctt ccgatcgcgt tcggccatct aacgccaccc cccccgccac gtcaacggac
     4081 gctctttcgt catggccccg atacccaaac cccagtcgaa agccgcgcaa gttgcaacta
     4141 gctgtattta catagttaat agtattttcc acgaacgtgg cgctttcggt tatccttctt
     4201 ggatgcggaa ttgtctgata gttgccttgt gtttttctgg attgctctgg atgggttgcc
     4261 aaaacaacaa gccgtcgtgc atccaagaat gcgaaaaagc cttcgagagc caagaaaaag
     4321 cgtgcggctc gaaaacccaa tccgaagcgg actcatgtag ccagaaggcg aaagaaagcc
     4381 gctggtcgtg cagaaaacaa tgcggtttgg acgaacccag cccaacgccg ctcgccagcc
     4441 ctgcagccac ccccaccgcg tcttcccctg ccataccgag tgtccttgac cttggaaaag
     4501 tctgcgatgg aaaggccgag cccacggctg ccgcggtcga caatacctcc ggtgcccata
     4561 acgccgccgt ggtcgccacc caaaacctcg gatcggattt gtatctcgtc gatacaatca
     4621 cgaacagcta tgacagcatc ggccccaaaa gcggcaaatc caaacaagcc gatgcttacg
     4681 ccctggtggt atgcgtgtcg gtcaaaaagc accaaaaagt gaaagaatgc ccctttgcca
     4741 agcatacctt ggaactacac gatgccacgt atcagatccg ggtcgtcgaa gcgcgtaccg
     4801 ccaacgaact caaaagcatt gacatagctg tcgcagccga tcgtcaatgc ccaccaacca
     4861 gcacctctca tcagcagcga gacatctccc ttccttcctt cgtcaaaacc gttctggaag
     4921 aggcggattt gcacgtccat ccatgaacgc tccccaagcg aggaaccggc gatactcctc
     4981 gagccgatag ccgctaccga cgtcgtcggg gacgggcgct tttgggagcg tcacgcaagg
     5041 aggacttgcg ggactttttc acggggttcg cggcgatcgc gctcgtttgt ggttcttcca
     5101 gcgcacgaag ggacttgagc gtatcgcgaa gacgagccgc ccgttcgaaa tccaatgctt
     5161 ccgcggccaa aaacatctcc gcgcgcatcg cctcgatccg ctcttgcaac gagatttcgg
     5221 tttcggctgc cgccgaactt tcccgtcgac gtgacggaat cgtgtaataa tcggtgtttc
     5281 ccgacgacgg agaaatatcg gcgatggcct tttttaccgt ttccggtgtg atgccgtggg
     5341 tttcgttgta ccgggtttgt cgttctcgac ggcgattcgt ttcctcaagg gcgcggttca
     5401 tcgcgtcggt gatgcgatcg gcatacatga tgactcgccc gttgacgttg cgggctgcgc
     5461 ggccgatggt ttgaatcaaa gagcgagcgg aacgcaggaa accttccttg tcggcatcga
     5521 gaatggccac aagagaaact tccggcaaat ccaatccttc tcgaagcagg ttgattccca
     5581 ccagcacatc gaattcccca agtctcaaat cccttagcaa ttcgatgcgt tcgagggttt
     5641 ccacgtccga gtgaagatag cgaacccgaa tcccaagttc ttggtagtaa tccgtgagat
     5701 cttcggccat tcgtttggtc aaggtggtga cgagcacgcg ctcaccggcc tggacccgaa
     5761 cgcggatttc accaagaaga tcatcgactt gccctgtcac ggtacgtact tcgaccctgg
     5821 gatccgtcag gccggtgggt cgaatgactt gctcgaccac ttctccagcc gtcaacccaa
     5881 tttcctcctc tccaggcgtg gcagagacga aaatgcactg tttgatacgc gattcgaatt
     5941 cgtcgtaacg caaaggccga ttgtccaagg cgctgggcag acgaaatcca taatcgacca
     6001 gggtgctttt gcgcgaccgg tcgccctgat acatcgcctt gatttgcggc aaagtctgat
     6061 gggattcgtc gatgatcaga aggaaatcgt cgggaaaata atcgatgagc gtaggcggcg
     6121 catccccagc ttttctcccg gatagatggc gggagtagtt ctcgattcct tggcagtagc
     6181 ccatctgctc gagcatttcg aggtcgtaca gggtgcgctg ctcgatccgc tgtttttcga
     6241 gcaaacgccc ttggccgtca aaaaactgcg atcgctcgag aagttcggcg cgaatggatt
     6301 cggcggcctt gcgcagttga gcctcgggtg tgacgtaatg cgaacctgga tacacatcgt
     6361 agcgctccaa ggtttgcaac acccgaccac gcatgggatc gatttgacga atcgcctcca
     6421 cctcatcgcc aaaaaactcg acacgaacgg ctcggtcttg ctcataagcc gggaagactt
     6481 ccaccacatc cccccgaacc cgaaaggtgc ctcggtgaaa atcgatatcg tttcgctcgt
     6541 attggatatc gactaacttg cgcagcagct cgtcgcgacg cagttcgact ccggtttcga
     6601 tgggaacgag caggcctgca tagctttccg ccgaaccgat gccataaata cagcttacgg
     6661 aagccacgat gatgacatcg tgacggctca agagcgcggc ggtggcggcg tggcgcatgc
     6721 gatcgatgtg gtcgttgatg atggagtctt tttcgatgaa ggtatcggtg acagggatgt
     6781 aggcttcggg ttggtaatag tcgtaatagg agacgaaata ttcgacggcg ttctggggga
     6841 acaggtcgcg catttcgcta tagagctggg cggccaacgt cttgttcggc gccatgatga
     6901 gggcgggtcg gttgacggtc gcgatgacgt tggcgatcgt gaatgtttta ccgcttccgg
     6961 taatgcccaa caaggttgtg aatcgttcgt ggcgacggag acggtcacat atttcttcga
     7021 tggctttggg ttggtcgccc ttgggaagaa aggaggaaac caattcgaaa cgaggcatga
     7081 tggatgaaaa cgagagcgtt ttttcgggca gagggaagga tgctcaatcg gaggcgctgt
     7141 cggagccggc gtccgaagag atgggggttc caccgcttgg ctccgtcacg catttggaca
     7201 tgtcttcaca gttgggggtt tcacgctcgt catcgccaca agcggcgagc aacagggcac
     7261 caacgagtag ccaaagtaca cgggacatca tttattctcc cgaggtttga aagatagggg
     7321 gacttgaaca tcggcacagc ggccatccgt taccggtggg aaacgttttc cgctgaacag
     7381 gctgctgaca caagaagtca cttgcgccga gcccacgctg tcttggacca cgctggcact
     7441 acagacattg ccgttagaag caacccgaac cgcgaccatc atgcgtcctt cgagcatagg
     7501 attggtgcgc aaagcgcgtt catagcaccc gcgggcggcc cccgcggtgc tggaaagagc
     7561 gctgcgtaac gcgggcggcg cgtttccatt gcaggggctc gaacataggt tcgttccgct
     7621 cgttgccgtg tttttctttc cgctgtcggg cccgctatct gggccggcct cgatttccgg
     7681 gggggccgga atatccaaaa tgggtggagg ctgggcggtt tcgaccgtca tcgactgaat
     7741 cacaggcgtc tgaaccggcg gatccttcct gctaaacttg aaccaaacca aaccaatgat
     7801 cagcagcaca agaatgatga tggccgcgat ataaggcgca ccgctcgact ttttcggcaa
     7861 cgtcgaagga atggacgggc gaggaggcga agaaggagtc atgcaggtcc ctcaaagaat
     7921 ctcgaagcgg tagtataccg attcaggttt cgcgttgtag cttatttttc ccgtttgcga
     7981 gagaaaagaa cgccagatgc gtatcaccat actgtcgaaa atcgtcgagc ccgaatccct
     8041 gtagcgaagg aaccggctga tcgctagggg attcaaaaat caattcggcg tggggtgata
     8101 agatcccttc ctccaccatg gcttcgaccg cgcgaagggc cgaacgatca cgaaccgcgg
     8161 aaaacggggg gtctacgaaa cacagatcaa aaggcgaaaa agcacgaaga acagaaaagc
     8221 tacgctctac tgcacgcaca acgacttgag ccaccgaagg gtcggtgagg gctagattaa
     8281 ctagattggc acggatgatt tgcgccgctg aaggggccgc ttcgacaaag acggtgcggc
     8341 gagcaccgcg ggacaaggct tcgataccca acgcccctga accggcataa aggtcgagca
     8401 cgttgcgctg ctggatgctc gagccaagca tgtcgaacaa ggcttctcgt actcgctcgg
     8461 tggtgggacg tgtggttttt ccagtcggaa ctcggagtct ccttccgcgc aatacgcctg
     8521 ctacgatacg catgggtttt gactctacag gcatcccgag ctgtgggggc gaggggaatt
     8581 aatatcaata aaaacgatta gttagaattt aagccggagg tccaagccac ccccgccacg
     8641 caccgccgtg ggaagaactt gcagttggtt ggcagtcgag ggcccctttt cgtcgggttt
     8701 ttcggtaacc aataggtatg ttcccgcgcc cacggaaacg gcgctcaacc cgtagaaaac
     8761 ccattggagt acggaaaact tatcagaggc ggcgcattta tcctcgacgg tttgggggga
     8821 agcggctccg gctttcccgg aatacacgcc atttttggcc gcttcgcata catctccttc
     8881 gacaccctct cggtaggcga taaacgcatt gtcgttgttg atgctgttga cttgcaacga
     8941 ggaatacaca ccaccaatag ccaaggcgcc gccagcgacc aaggcggaat aggcaccgat
     9001 acggcgatag ttcggtcctt ccgaggaatc ggcgttttct tgagcgacgg gatgcaaggt
     9061 aagggaaacg cttccgttgg ggtggacggt cacgtcgccg gaaacagggg cataaccagg
     9121 ggctcgtact tcgatccgac gggtgccggc gggcacgaag atggtggctt ttccgttttg
     9181 gatggtgccc acaggcttac cgtcgacgag cacctgtccg ttgacgtcgc cggctctgat
     9241 atcgacggag cctttggggg gaccgccggt cagggtggcg agagcttccc ggacgatttg
     9301 gcgaagggtt tcgtcgttag cttctgttaa attcgacgcg taggaaaggt cgactttcgt
     9361 ttgcccttgg tcccgttgcc agaggtgcaa cgtgccgact gcttttccag atttttcgtc
     9421 gatagttccc cagatatagc ggtctgcttt gatttcttgt gcgatgcgag cttcgcaagc
     9481 ggcatccggt ggccaggggc aacgtagtgc catggtgagc acttcgaggg aataatcgcc
     9541 ctcggcgagg gaccagcccg ctaaattgcg cacttccgct tgcaacgcgg tcgtcaacgc
     9601 atcggcctgg gcgtcggaat cttcggtttt gatgcggacg acggtaatgg ccggcgtatc
     9661 gggactagga gggtccgcaa agccgaccga gggacttgcg aggatgagga aggggccgag
     9721 caacatcaaa ccacggcgca tccgatgcct ccgctgcgag ctggttgtgg gacgtaatga
     9781 ttcgtgcata tccgattact tggctttttc tgcgacgtct tgctctcgct gggcgattcg
     9841 ttctttgact ttggcgtatt tcgcttcgag ttcctggcgt ttttcgatga tgcgaatgcg
     9901 ctcggtgggg gtggcaggat agcctttggc cttgggttcc gatgcgatga agtcggcata
     9961 cgctttccat gccttgatgg catcgtccca tcggccttgt tgctcgcgaa gatccgcgag
    10021 gacgaaaaga attttcgcgc gcacgaccgg atcgctattc gcgtatcgga gcgcggtttg
    10081 ccacgcccct tcggcttggt cgattttttt gtcggccagg aaggcttgtc cgagcatgta
    10141 gtgtccaagc ggacgattcg gagttttttg gatcgcctct tggtaggtgg agatagccgt
    10201 agggaagtct cttgcgacgt atgccgaact tcccttgacg atcagttcca tgaaaggact
    10261 gatgccggtt atcccttttg gatcgcgtcg cttggtggtt gcagccttgg agtcttgggt
    10321 ctgtgccttg gtgtcatcag ccaagctgat ggacgttacc gacatcatca atccgagcgc
    10381 ggcccacaaa ggccaaccat gttgcatgat gagaatcctc ctgacgaacg gtgggagtct
    10441 accacggaag tgccacaggc aaagagcaaa gcaaaggtct tgagcgaagg agccgaattg
    10501 ggtcgttgca ttccgataat cgactgggtc tgaggagcgg acgggctggt tcccaccgcg
    10561 ccggtggccg aaggccccgt ggcaatgggc tcattctttg ggtaggacga ggcgaaaggt
    10621 aacggtcgta tcatcgcatt ccacttcgac ttttccgcga tgggcttcgg cgacggcacg
    10681 cacgatgggc agaccgagac cggttccgcc tttatcgcct cgcgaggtca caaatctccg
    10741 aaataaccgt tttcggacat gaggaagaag agttccagga ttggagacgg tgaccatggc
    10801 attgggaccg agggtatgaa gggaaatgcg gggcggttgc gagccatctc catgagaaaa
    10861 ggcgttgtga acgaggttgt cgatcgcacg cgccaaccac cggccatccc cacggatacg
    10921 tacgggaccg ccaggctcga acaggacttt gccccgggtc gcgtgcgatt cgaggatgga
    10981 ccgaaccacc gcctgcaaat cgaccgaatc gaagttctcg atgcctcgcg actcgatacg
    11041 cgccagcgac agaagctccg aaaggatgac ctcgatgcgc ccggtagcct cccgaatgcg
    11101 tttgacgaat cgagccgcct cttcgggctc tgccaacgcg ccgtcatcga gcacttccgc
    11161 cgaagcgcga atggcggcca cagggttttt gagttcgtga gacaagtcgg cagcaaagct
    11221 ttcgacgtaa ggtaagccat cgagttgtcg acgcattttg tcgatggctt tggtgagtcg
    11281 tttgacttcg agtccgtggg gcaccggtgg ggtgacggcg cgatcccctt cgctgactcg
    11341 ttctgcgaag tgggtgagag attcgatggg agcagcgatg gctctgccga tgagcgcggc
    11401 cgcaagcgcc gccgctgcac caagaatggc gctgatgaca aggaccgtgg gcgcaaaatc
    11461 ggccagcagt ctgcgcatga tgacggtttc tttgacgact tgcacatggc cggccacacc
    11521 gtggcgtgta tgaatggggg cgcgcacggc gacggttccc ggatcatcgg ggtttgggcc
    11581 atcgatgaac atcaccttgc ccgatgcatc aaaaagagcg agacgagaac gttccggact
    11641 tccgcgtctt cgttcgtatt caagcacctc gacgataccg tggagcgagg cgccggtagc
    11701 atcg
//
LOCUS       DAOSOR010000115        11676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5479_length_11676_cov_3.743654, whole genome shotgun sequence.
ACCESSION   DAOSOR010000115 DAOSOR010000000
VERSION     DAOSOR010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11676
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5479_length_11676_cov_3.743654"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="PK710_08410"
     CDS             complement(<1..948)
                     /locus_tag="PK710_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT09778.1"
                     /translation="MADVELRNVSRQFEGAPSRAVDDLSLHVASGEFLVLVGPSGCGK
                     STALRMIAGLEDPDDGQISIGGRPMNRVAPQDRDVAMVFQGYALYPHMKVREIMGFPL
                     KMRKVPAAQREQKVKEAADMLSITRLLDRRPDELSGGERQRVAMGRALVRSPKVFLFD
                     EPLSNLDAALRGEIRVEIGSLVRKLGATTVYVTHDHVEAMTLGHRIAVMRGGKLIQLG
                     TPREVYEHPATAFVASFLGAPAMNLTSATRENDHLTVHGIRLRCLGDSPKRLLLGIRP
                     EHVKVHGPVPDGNLRVEAIVTTVEPLGAETFVHLDAGGAA"
     gene            complement(1128..1403)
                     /locus_tag="PK710_08415"
     CDS             complement(1128..1403)
                     /locus_tag="PK710_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09779.1"
                     /translation="MPRRFLILFPLFSFLACAESVHPRVGTGIPAPTWIEVEPKIPFE
                     QDDPEAEEPASEDVSTPSEPPAAESPNEDAPAGTPPEPSAPEEEKLL"
     gene            complement(1477..1752)
                     /locus_tag="PK710_08420"
     CDS             complement(1477..1752)
                     /locus_tag="PK710_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09780.1"
                     /translation="MTESSGPLANLETNDIIAIVLSIFVPGVGHILLGQKTKGIVILG
                     AVILSCGVGYLVSLLIAIDALCVARISKERPVQEWEFFPEHKRWLGV"
     gene            complement(2186..4414)
                     /locus_tag="PK710_08425"
     CDS             complement(2186..4414)
                     /locus_tag="PK710_08425"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HOT09781.1"
                     /translation="MNSKLHISLLALASLSLLATGCSDSEDNRTEPFGVARASLTKAQ
                     DCNDLESRIKADALAKMNARIDAEIAMWDGGGWAHPTPDYDMGAPEAGNSQGTGGTGS
                     SNDARQQESAKEYSGTNTQVDGVDEADIVKTDGKYLYLLHGQSFLVLNAWPATQLAQS
                     SSFAVEGTPSEMYVTDDGKVVIYSNVNGTAIYAAAGISPRNSYDDSYYGYYGNGAEPY
                     PGGPEYMSNPLTKITVLQLQNAQPTVLSETYFEGSYVSSRRVGSHVRTVLEGASFGPN
                     LSYWPDDYPSTKTEWVAAYEKLRTENAAKIRNSTLSDWLPYSMVRSETGVQAHLARCD
                     EFYVPTTASTVYGMTQIQSLDLAAPQLPPVGVSIIGAVDTVYSNLDTLVIAARAWVDP
                     TVYSWGTSRTDGDGVVSSPPMADGVATTRQALTEEPISTAYTHLHSFDLTFDPLRPQY
                     VASGTVVGTILNQFSLDVHNGNLRVATTDERVNVDWDNRQTNHVFILSPQNDELQIVG
                     SLRNLAPTERIYSARFMGDRGYLVTFRQVDPLFVLDLANPAAPAVLGELKIPGFSEYM
                     HPLDSNHLLTIGQDDGLALQIFDVSDPVNPVQKHKYVFSNEQIYGYSEAQTNHKAFTY
                     YDSHGLLAFPFVGWDNAGKMRSTLELFDVNKDTGFHRRGGVDHTALFDDVKDPYGYCG
                     GYYGVDVRRGLFLEDFVYSISYGGVVVNHIDDLNNPVASFALEAPTNTSYPGCYGNYE
                     MD"
     gene            complement(4536..5555)
                     /locus_tag="PK710_08430"
     CDS             complement(4536..5555)
                     /locus_tag="PK710_08430"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT09782.1"
                     /translation="MRYTSLVPRTPEPPQHGLVDRILGQGQRIHIPLGATCNNRCLFC
                     MEDNRKARALVNGALTPERVRWILDSHRDAEELCFTSGEPTLHPMLPTFIQWAKDAGC
                     KRVSLMTNGRRLAYAPYTKALVRAGLQLVYISIHGVSAKMHDGLTRTPGSFVQTLEGV
                     RIAASFSSLRVHTSTVVTRRNMSYLFEIYDKLIEMGVQQTVFNALQIQGGASKHFPSM
                     VPQYREIRHQFERLLHLARNGGARAFLVDVPPCISHGLPDINRGFVEKHVHFEPETHG
                     VSPPGATLCEGSDGVCAIRTDSLDATFRTFGPHCPSCRYKPVCPGVFPRYVQQFGWDE
                     FIPVE"
     gene            complement(5647..6147)
                     /locus_tag="PK710_08435"
     CDS             complement(5647..6147)
                     /locus_tag="PK710_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09783.1"
                     /translation="MSQRAKGKLNRSKQQASDSLEPADPWCRPESEYRLFPQEHDDEP
                     QEDEESLPRVVVGTLADLDVETAHPDRPIWILLEEHHDEVGVFERYPEAPMPSATIAS
                     LLSAIRQFTEKHASGLQTLLGPTDRLRMGFHTSATLEEVVESFEDDGFDVLLGIHNGQ
                     VVLPEP"
     gene            complement(6156..7163)
                     /locus_tag="PK710_08440"
     CDS             complement(6156..7163)
                     /locus_tag="PK710_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOT09784.1"
                     /translation="MRSFLFVSCLALWVVTACSAGGEQQPLSNAGGAGSNTPDNPATG
                     GSPSDPTEAGAFFDSSMNQDTSAVCTDSIDVVFVLDVSSSMGFVLDKLGNEIDGVVAE
                     AQKLTTNSGAHPPHFGLIPFVDNHVIDLGGVEDGNAVHLDAQSLRNAFKFHRTNFTNP
                     NRNPGDGLGGPTMQNPICEENALDALYAAAKDFPWRANASHIIILATDDTFLERPDNY
                     GDRDNDGLTNKTDYPREGDYPARWTVPETVEALKVAKIRLFTFTSRGDAERHCGTGRR
                     LPWEARAAGWSAPYFNHPPLPDSTDGANFDLDLVRKSTLSLSSTINDIVLNTYCNPPV
                     Y"
     gene            complement(7285..9165)
                     /locus_tag="PK710_08445"
     CDS             complement(7285..9165)
                     /locus_tag="PK710_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT09785.1"
                     /translation="MNAAAGRLTQSVVGALILAGASTGIALALAAPRFLLIEPDTTQQ
                     LLERGTIVGLLEVLALCLLAWLRLYRHRFTIRALGLGSGSLEPDALIALGKEPGAITI
                     RGIVITIVGALSTLVQPVRPESLELDAAVSLILLALAFSTTAGVALYVFIRRRISQAM
                     LLAPEEAMAEVMERVLATETTSRWTMRRVLGAVLAPTAFVGVSAVLIVHAHVRTFESN
                     GRLATATAIAQSALEPIPGPVPTAGEDDAAQVAANFGYAIRLSHGTSPFQVRRFRTGH
                     MMVTVPLDLGYATITFPATTVLVLTASSILAAVFASLLAVVLGIRIGRTLAADLEQAA
                     HQVRWLGTDTVLRGFTRVARPARFEAVANLGEAIEKLADRFRVFASAQEKAIEAREAT
                     RRMRGLLFASVSHDLKSSLNSILGFADIIDSERITPQQAESLHVIQSRGRELLDLIQT
                     ILDGARVETGQIALAKRNTTVQDVIASALDQLQSTFSDNAIPIINEATFAPWIVDVDP
                     ERTADALRALLRHALRTTTSGVIHLRAVISPSGRDVLIDIHVPSRTIPASKLARFLLP
                     DNESTMPRSAGGLALGLSLARSLIVMHGGNVEPLDTVSGVVLRVALPLEGSYSENHDK
                     NQ"
     gene            complement(9162..10676)
                     /locus_tag="PK710_08450"
     CDS             complement(9162..10676)
                     /locus_tag="PK710_08450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT09786.1"
                     /translation="MASFQQRIQRLRLPLVPLLPAIVVAIGAFTAIVAGFLGLAHLRS
                     MSDGIAQDRADVLASTLAARIRTTDGTDQPMVIRRAARRSGAEILLLTHDGISRMDDN
                     DAGLDTPQLLQVLIEGRGEVITRIGRAKYAARPIGPPFQNLALVVFVSAPAQPEGATS
                     LIRSLLVVTALLLGVAVFVAFVFVRELRGDVDFVRSEIDRMAEPDAAPTGRPIRVRMT
                     DQVGVLTHAFNLLVDRFAAAERAYQLDLDHAASLDRDRAAFLGVLSHELRTPLNAILG
                     FTDVLLSEVDGPLDADTRENLEHVRASGSHLRGLIDDILELSALESGQLRLSRNRVDL
                     RAVADDVMREASARIGSKNIALHVEGPSPAYAYVDERRMWQILSNLVSNAIKFTTSGD
                     VRINIALELGQIHISVSDTGPGIDAEDREAIFDEFRQVGPLTARAKGTGLGLFIARRL
                     VTMHGGTIMVESEVGQGSCFHIRLPAWSVDTGGFDGFDVVTTGLLPVANAGESQ"
     gene            complement(11134..11451)
                     /locus_tag="PK710_08455"
     CDS             complement(11134..11451)
                     /locus_tag="PK710_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09787.1"
                     /translation="MKLYDLHGKDGAEKLSESFKNPNNGSHTSNQVTITNRLDFNFNR
                     GRTTFVMKKGKYRVTGGEPEAVYDSQDGSKTIVLVRVNIPRKTTSGPGKVVVSIEPDP
                     SSP"
     gene            11455..>11676
                     /locus_tag="PK710_08460"
     CDS             11455..>11676
                     /locus_tag="PK710_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09788.1"
                     /translation="MGTIGNEGAGPPLVGMFESDHSRALISPGLRTHDRRGKDRFIID
                     DDTFMFMMVAYQLKVSRMIFGGNLRFAGVE"
BASE COUNT         2535 a         2868 c         3836 g         2437 t
ORIGIN      
        1 agcggctccc cccgcatcga gatgaacgaa agtctccgca ccaaggggct ccacggtagt
       61 tacgatggcc tccacccgaa ggttcccatc gggcacggga ccgtggacct tcacatgttc
      121 cggtcgaatg ccgaggagca ggcgcttggg gctatcgccc aggcaacgca atcggatacc
      181 gtggacggtc aagtggtcgt tttcccgtgt ggctgaagtg aggttcatgg caggggcgcc
      241 gagaaacgag gccacgaaag cggtagcggg atgttcataa acctcgcgcg gggttccgag
      301 ctgaatgagt ttgcccccgc gcatcacggc gatgcggtgg ccgagggtca tggcttccac
      361 gtggtcgtgg gtcacgtaca cggtggtggc tccgagtttc cttacgagag agccgatttc
      421 tacgcgaatt tcacctcgaa gggcggcatc gaggttggac aagggctcgt cgaaaagaaa
      481 gaccttcggg ctgcgcacga gggcacgacc catggcaacc cgttgtcgtt ctcctccgga
      541 aagttcgtca ggacgacggt cgagaagcct tgtgatgctc agcatatccg cggcttcttt
      601 cactttttgt tcacgctggg cggctggaac cttccgcatt ttcagaggaa agcccatgat
      661 ttcacggact ttcatgtgcg gatacagcgc atagccctga aataccatgg ccacatcgcg
      721 atcctggggg gctacccggt tcatggggcg cccaccgata ctgatttggc cgtcgtcggg
      781 gtcctcgaga cccgcgatca tgcgcagagc cgtggatttg ccacagcccg aaggacccac
      841 aaggacgaga aactcgccgg aagccacgtg aagggagagg tcatcgacgg cgcgagacgg
      901 tgcgccttcg aattgtctcg agacgtttcg cagttcgaca tcggccacga gacggtaggt
      961 agcgaggtcg agggaaaggg gccaagttgc gtgctcaggt cgtcggccat cgtcgccgtt
     1021 gactcacctt tgcggtccgg gcggagtcga gcgagggtgt ccgtgtcatg aaagggcgaa
     1081 tccggggtgt gggggcactg ggccaccaca agcataacag attgaattta caataatttt
     1141 tcttcttccg gcgcagaagg ttccggtggg gtcccggcgg gagcgtcttc gttgggagac
     1201 tcagccgcgg gaggttccga aggggtggag acatcttcgg aagccggttc ttcggcttcg
     1261 ggatcatctt gttcaaaagg gattttcggt tcgacttcga tccacgtggg agcaggaatc
     1321 cccgttccga cgcgcggatg gacggattcg gcacaagcta gaaaagaaaa caggggaaat
     1381 agaatgagaa agcgccgtgg catcggcaga gggtaggcgc gaccgaggct cgtcgtcaac
     1441 gattgtcgtg ggcaggcggg aacaagagga agggagtcag actccgagcc atcgtttgtg
     1501 ttcaggaaag aattcccatt cttgcacggg acgctctttg ctgattctcg caacgcaaag
     1561 ggcatcgata gcaatgagca acgaaacgag atagccgacg ccacaagaaa ggatgacggc
     1621 accgaggatg acgatgcctt tcgttttttg gccgagcagg atgtgcccca cgccggggac
     1681 gaaaatactc agcacgatgg caatgatgtc gttggtttcg aggttcgcaa gagggccgct
     1741 tgattcagtc ataccggggg tcttccttgg cttcggtgag cgggagatgt aaagacaaga
     1801 gggagcgaac gcaagggaaa aggcatgaga aaaaccggtt tgcttcgtgg cggcagggcg
     1861 attatcacgc acgtctgtcc gtccgtagtc cgggcacgtt ggcgatatgt tacaggctcg
     1921 gccgcggcct cacgaccggg gtcgatgttt tcaagaaagg gacgagaagg tttggggggg
     1981 tgagcgaacg cttcgatttt tcctgaatta atacagttac ttgaacacac caggcagatg
     2041 ctcgcaggtt acaaggcctt atccagaagc tcgactaggt attcagcctg cgcgagatgc
     2101 gtgggccgtg ggccatgagc gtttgggtgg ggggactaaa ggaacgtcga aagaggggca
     2161 gagggaggga caggctggag aatcatcaat ccatttcgta gtttccgtag cagccgggat
     2221 acgaagtatt ggttggggcc tcgagggcga aggaagcgac ggggttgttg aggtcatcga
     2281 tatggttgac cacgacaccg ccgtaagaga tggagtagac gaagtcttcg aggaaaaggc
     2341 cgcgacgtac atctacaccg tagtagcctc cgcaataacc ataggggtct ttcacatcgt
     2401 caaacaaggc ggtgtggtcc acgccgccgc gacggtggaa gccggtgtct ttgttgacgt
     2461 cgaaaagttc gagggtgctt cgcatcttgc cggcgttgtc ccatcccacg aaggggaatg
     2521 cgagcaagcc atgggaatcg tagtaggtga aggccttgtg gttggtctgg gcctcggagt
     2581 atccgtaaat ttgttcgtta gaaaacacgt atttgtgctt ttgaacggga ttgacgggat
     2641 cggagacgtc gaagatttgc agcgcaagtc cgtcatcctg accgatggtg agcaggtgat
     2701 tgctgtcgag cggatgcatg tactcggaga agccggggat tttcagttca ccgaggaccg
     2761 cgggggcggc ggggttcgcc aagtcgagga caaagagggg gtcgacctgg cggaaagtga
     2821 cgaggtagcc gcgatctccc atgaaacgtg cggagtaaat acgctcggtg ggggccaagt
     2881 tgcgcaagct tccgacgatt tgaagttcat cgttttgcgg actgaggatg aaaacgtgat
     2941 tggtttgcct gttatcccaa tcgacgttga cccgctcgtc ggtggtggcg acgcgcaggt
     3001 tgccgttatg cacatcgaga gagaattgat tcaagatcgt tccgacgacg gttccggaag
     3061 cgacgtattg tggccggagc gggtcgaagg tcaggtcgaa tgaatgcaga tgggtatagg
     3121 cggtgctgat gggttcttcc gtcaaggctt ggcgtgtggt ggcgaccccg tcggccatgg
     3181 gtggagagct gacaacgcca tcgccatcgg ttcgggaggt accccaggaa tagaccgtgg
     3241 ggtcgaccca tgcgcgggct gcgatcacga gggtgtcgag gttggagtag acggtatcga
     3301 cagcgccgat gatgctgacc ccgacggggg gcagttgcgg ggcggcgagg tcgagggatt
     3361 ggatctgggt catgccatag acggtactcg cggtggtggg cacgtagaat tcgtcgcagc
     3421 gggcgaggtg ggcttgcact ccggtttcgg atcgaaccat cgagtagggg agccaatcac
     3481 tcagtgttga gttacgaatt ttggctgcat tttcggttcg gagtttttcg taagcggcga
     3541 cccattcggt cttggtcgaa gggtaatcat caggccagta gctgaggttg ggaccaaacg
     3601 aggcgccctc gaggaccgtg cgaacatgag atccaacccg tcgcgacgac acatacgatc
     3661 cctcgaagta cgtttcactg agcacggtgg gctgggcatt ttgcagttgc agaaccgtaa
     3721 ttttggtgag tgggttgctc atatattcgg ggccaccggg ataaggctcg gcgccgttcc
     3781 cgtaatagcc gtaatacgaa tcgtcgtaag aattgcgggg ggagatgccg gcagcggcgt
     3841 agatggcagt cccattcacg ttagagtaga tgacgacttt gccatcatcg gtgacataca
     3901 tttcggaggg ggtgccttcg acggcaaagg aggaggattg ggcgagctgg gtggcaggcc
     3961 aggcgttgag tacgaggaag ctttgtccgt ggagcaagta gaggtatttc ccatcggttt
     4021 tgacgatatc ggcctcgtcg accccgtcga cttgtgtatt ggtcccggag tattcttttg
     4081 cggactcttg ctgacgagca tcgttggagg agccagttcc gccggtaccc tgggagttac
     4141 cggcttcggg agcacccata tcatagtccg gagtaggatg ggcccagcct ccgccgtccc
     4201 acatcgcgat ttcggcatcg atacgagcgt tcattttggc gagggcatcc gctttgatcc
     4261 tgctttccag gtcattgcag tcctgggctt tggtcagaga agcacgggca acgccgaacg
     4321 gttcggtccg gttatcttcg gagtcggagc aaccggtggc aagcaggctg agcgacgcga
     4381 gggccaaaag actgatgtgg agcttactat tcatgagatc acctctggcg ggtaggataa
     4441 gcagctttcg ggccaacctg ataagctaca aaaattggtg tgtggaacga accattgtga
     4501 caaccgtgtc aactgtgcca accaatggca cagaatcatt cgaccggaat gaattcgtcc
     4561 catccgaatt gttgcacata gcgtgggaat acccctgggc agacgggttt gtagcggcac
     4621 gaggggcagt gggggccaaa ggttcgaaag gtggcgtcga ggctgtccgt acgaatggca
     4681 catacgccat cactaccttc gcagagtgtg gcaccggggg gggaaacgcc gtgggtttcg
     4741 ggttcgaagt ggacatgttt ttcgacgaat ccccgattga tatcgggcaa accatgggag
     4801 atgcaggggg ggacatcgac caaaaaagcg cgggctccgc cattgcgggc gaggtgcagc
     4861 agccgctcga attgatggcg tatttcacga tattgaggga ccatcgatgg gaagtgcttg
     4921 gatgcccccc cctgtatttg caaggcattg aagacggtct gctgcacgcc catctcgatt
     4981 aatttatcat atatttcaaa tagatagctc atatttcgtc gcgtgacgac ggtcgaggta
     5041 tgaacgcgaa gcgatgaaaa agaagcggcg atgcgcacgc cttccaacgt ttgaacgaaa
     5101 cttccggggg tgcgagtcaa tccatcgtgc atctttgcgg atacgccgtg aatcgagata
     5161 tacacgagtt gcaaccccgc gcggacaaga gctttcgtat agggggcgta ggcgaggcgt
     5221 cgcccattgg tcatgagact gacccttttg catcccgcat ctttcgccca ctggatgaac
     5281 gtgggcagca tgggatggag ggtgggctca ccggaggtaa aacaaagctc ctccgcatct
     5341 cgatgggagt ccagaatcca gcggacgcgt tcgggggtca aggctccgtt gacgagggca
     5401 cgggcctttc ggttgtcttc catgcagaac aggcagcgat tgttgcaggt cgcgccaagc
     5461 ggaatgtgga tacgctggcc ttgacccaga attcggtcga ccaatccgtg ttgcggaggt
     5521 tcaggggttc gaggcaccaa ggaagtatag cgcatatcgt gaggtcaaat catgagagac
     5581 aacccttgcc gacgatgctg agcgcctcgg gcggccaccg caaacatcgc acgccaacgg
     5641 cgccatttac ggttcgggca acacgacctg cccgttgtga atccccaaca gcacatcgaa
     5701 tccatcatcc tcgaaagact cgacgacctc ttccagcgtg gcgctcgtgt gaaatcccat
     5761 acggagcctg tcggtggggc ccaacagggt ttgcaggccc gaggcatgtt tttcggtaaa
     5821 ctgccggatc gccgaaagca acgaggcgat ggtagccgac ggcatgggtg cttccggata
     5881 tcgttcgaat acccctacct catcgtgatg ctcttccagc aaaatccata tcggtcgatc
     5941 gggatgcgcc gtttccacgt caaggtccgc caaggtaccc acgacgacgc ggggaagact
     6001 ctcttcatct tcttgcggtt cgtcgtcatg ctcttgtggg aaaagccgat attcgctttc
     6061 aggcctacac cacgggtcag cgggttcgag agagtcactg gcctgctgtt tcgaacgatt
     6121 gagttttcct tttgcgcgtt gggacatgga taagctcaat agacaggggg attacaatag
     6181 gtattcagca cgatatcatt gatcgtgctc gacagcgaga gggttgattt tcggacgaga
     6241 tccaagtcga agttcgcgcc atcggtgcta tccggcaagg gaggatggtt gaagtaagga
     6301 gcggaccaac cggcggcgcg cgcttcccaa ggcaaacgtc gccccgtgcc gcaatgccgc
     6361 tcggcatcac cgcgggacgt aaatgtaaac aatcggatct tcgcgacttt cagggcttcg
     6421 acggtttcag ggacggtcca gcgggcggga taatcgcctt ctcgtgggta atccgtcttg
     6481 ttggtgagac catcattgtc tcgatccccg tagttgtcag ggcgttcgag gaaggtatca
     6541 tcggtggcga ggatgatgat atgggaagcg ttggcacgcc aaggaaaatc cttggcggcg
     6601 gcgtagaggg catccagcgc gttttcctcg caaatggggt tttgcatcgt gggtccaccg
     6661 agaccgtccc caggattacg attgggattg gtgaagttgg ttcgatggaa tttgaaggcg
     6721 ttgcgcaacg attgggcatc gaggtggaca gcattgccat cctcgacacc ccccaagtcg
     6781 atgacatgat tgtcgacaaa aggaatcaac ccaaagtggg gaggatgggc gccagagttg
     6841 gtcgtcaatt tttgggcttc ggccacgaca ccgtcgattt cattgccgag tttgtcgagc
     6901 acgaatccca tggacgagga aacatccaga acgaagacca catcgatgga gtcggtacaa
     6961 accgcagaag tgtcctgatt catcgagcta tcgaagaagg cgcctgcttc ggtgggatcg
     7021 gagggcgatc caccggtggc tgggttatcg ggagtattac ttcccgcccc gcccgcgttc
     7081 gacaagggtt gttgctcccc ccccgcgctg caagccgtaa ctacccacaa cgcaagacac
     7141 gagacaaaaa gaaaagaacg catgagtgta ttgtttcagc ccaacccagt tcgagcaacc
     7201 ccaattaccc aacctcgaca cgataggagc atcgtcggaa caaggatcga ttcctttgcc
     7261 cctcctgggc tgaagaaatg caatctattg atttttatca tgattttctg aatatgagcc
     7321 ctccaagggt agagcgactc gcaagacgac tccggagacg gtatcgaggg gttcgacatt
     7381 tccgccgtgc atgacgatga gagagcgagc gagagatagg ccgagggcga gtccccccgc
     7441 ggaacggggc atggtgcttt cattgtctgg caggagaaag cgggccaatt tggaggcagg
     7501 aatggtgcgg gaagggacgt ggatatcgat gagcacatcg cgtccggaag gggagataac
     7561 ggctctgagg tgaatcacgc cggaagtggt ggttcgcagg gcatgacgga gcagggcgcg
     7621 cagggcatcg gcggttcgtt cggggtcgac atcgacgatc caaggggcaa aggttgcctc
     7681 attgatgatg gggatggcgt tatcgctgaa cgtggattgg agttggtcca aggcggaagc
     7741 gatcacgtct tggacggtgg tatttcgttt ggctagggcg atttggccgg tttcgacgcg
     7801 agcgccatcg aggatggttt ggatcaagtc gagcaattcc cgcccacggg attggatgac
     7861 gtgcaggctt tcggcttgtt gtggggtaat gcgttcggaa tcgatgatat cggcgaagcc
     7921 caagatggaa ttgaggctac ttttcaggtc atggcttacg gaagcgaaga gcaatccgcg
     7981 catgcgccgg gtggcctcac gcgcttcgat ggccttttct tgtgccgaag caaaaacgcg
     8041 gaagcggtcc gcgagttttt cgatggcttc cccgaggttc gcgaccgctt cgaagcgagc
     8101 gggacgagcg actctcgtaa agccgcgcag gaccgtatcc gtgcccagcc atcggacttg
     8161 atgggcggcc tgttcgaggt ccgcggcgag ggtgcgtccg atgcggattc ccaacacgac
     8221 ggcaagcagg cttgcgaaaa cggcggcgag gatagacgag gctgtgagaa cgagcacggt
     8281 ggtggcggga aacgtgatgg tcgcatatcc caagtcgagc gggacggtca ccatcatgtg
     8341 tccggttctg aaacgtcgca cctgaaaagg cgaagtccca tgggacaatc ggatggcata
     8401 gccgaaattg gctgcgactt gcgcggcatc gtcttcgccc gcggtgggaa cagggcccgg
     8461 aatgggttcg agggcggatt gtgctatggc tgttgcggtg gccaatctac cgttggattc
     8521 gaacgtccgg acgtgggcat gaacaatcaa caccgcgcta acgcctacga aagcggtggg
     8581 agcaaggacg gcccccagga cccgtcgcat agtccagcgg ctggtcgttt cggtggcgag
     8641 gacgcgttcc atgacttcgg ccatggcctc ttcgggggct agaagcattg cttgagagat
     8701 acgtcgtcga atgaaaacat aaagggcgac gcctgcggtg gtactgaaag cgagggcgag
     8761 aagaatcagg ctcacggcgg cgtcgagttc gaggctttct ggccgcacgg gctgcaccaa
     8821 ggtagacaaa gcccctacaa ttgtaatgac gataccccgg atggtgatgg cgccgggttc
     8881 tttgccgagg gcgatgaggg catcgggttc gaggcttccc gagcccaagc caagagcgcg
     8941 aatggtgaaa cgatggcgat aaagtcggag ccatgcgaga aggcaaaggg cgaggacttc
     9001 gagcaagccc acgatggtgc cccgttcgag gagttgttgg gtagtatccg gttcgatcag
     9061 caagaatcgg ggagctgcca gagcgagcgc gatgccggta gaagcgccag cgagaatcaa
     9121 ggcccccacg accgactggg tcagtcttcc ggcagcggcg ttcattgcga ttctccagcg
     9181 ttagcgaccg gcagcaaacc ggtcgtcaca acgtcgaatc catcgaatcc cccggtatcc
     9241 acggaccaag cgggcagacg gatatgaaaa cacgagcctt gtcccacttc gctttctacc
     9301 atgatggttc ccccatgcat ggtcacgagg cgtcgggcga tgaacaggcc aagtccggtg
     9361 cctttggcgc gggcggtcag gggcccaact tggcgaaact cgtcgaaaat ggcttctcga
     9421 tcttcggcgt cgatacccgg accggtatcc gacacgctga tatggatttg tccgagttcg
     9481 agggcgatat tgatgcgcac gtcgccgctc gtggtgaact tgatggcgtt gctaacgagg
     9541 ttgctcaaga tttgccacat ccttcgttca tccacgtagg catacgcggg gctgggtccc
     9601 tcgacatgca aggctatgtt tttacttccg atcctggcgg acgcctcccg catgacatcg
     9661 tcggcgacgg cgcgcaggtc gacgcggttg cgagacaggc ggagctgtcc ggattcgagg
     9721 gcggacaatt cgaggatatc gtcgataagg ccgcgcaaat gcgagccact ggctctgacg
     9781 tgctcgaggt tttcccgggt gtctgcatcc aaagggccat cgacttcgct caacagcacg
     9841 tcggtgaagc caaggatggc attgaggggg gtgcgcagtt catggcttag cacgccgaga
     9901 aaggcggccc ggtcgcggtc gagggaagcg gcatggtcca aatcgagttg gtaggcgcgt
     9961 tccgcggcgg caaaacggtc gaccaagagg ttgaaagcat gcgtgagcac accaacttga
    10021 tcggtcattc gcacccgaat gggtcttccg gtgggtgcgg catccggttc ggccattcgg
    10081 tcgatttcgg aacggacgaa gtcgacatcg ccgcgcaact cacggacgaa cacgaaggcc
    10141 acgaagacgg cgacaccgag caacagcgcc gtgacgacaa gaagggatcg aatcagcgag
    10201 gttgcgcctt ctggttgtgc tggggctgaa acgaaaacga cgagagcgag gttttggaag
    10261 ggaggaccga tgggccgagc ggcatatttg gcgcgcccga ttcgagtgat gacttcgcct
    10321 cgtccttcga tgagcacctg aagcagttgt ggggtgtcga gtcccgcatc gttgtcgtcc
    10381 atccgcgaga tgccgtcgtg ggtgagcagg aggatttcgg cgccggaacg tcgggcggca
    10441 cgacgaatca ccatgggttg atcggtgcca tcggtggttc gaattcgcgc ggcgagggtg
    10501 ctggcgagca catcggcgcg atcttgggca atgccatcgc tcatggatcg caggtgagcg
    10561 agtccgagaa agccggccac gatagcggta aatgctccaa tggcgacgac gatagccggc
    10621 aacaggggaa cgaggggaag acgcaatcgt tggatgcgtt gttggaacga cgccacggca
    10681 ggcatcttag tgtggacgaa ggaatgaatc gatgggttgg ggaggaattt ggaaggcaag
    10741 gggcaagggg gtggaacttt ggtaggatac gaacctaccc ctttccatga cctttttctc
    10801 gatggggaag aaacgttgag gggctggctt tctggtgagt gtggtgagat cggagtatcc
    10861 ccgggatggg cgcaaagctg tagtcggttt tcgatggaat ggcggcgtgg gcaggtcagg
    10921 aacacgagag gtcatgagat gggccgcggc aaaaggcgcg cagggagcct gggcgataga
    10981 agataaaaca atctataatt tcaatatgtt acataaatta cgacggatga ttcggtgggg
    11041 tccttggaag aaaaggttca cgaatttcgc agacgctttg atcgccagtt ttcgtgagct
    11101 tttttttcgg tgggaaggcc agcggacagg cagtcagggg gaggatggat cgggttcgat
    11161 ggatacgacg acttttcctg ggccggaagt ggttttgcgg gggatgttca cgcgcacgag
    11221 gacgatggtt ttggagccat cttgactatc gtagacggcc tcgggctccc ctcctgtaac
    11281 gcgatacttt ccttttttca tgacgaaggt ggttcggccg cggttgaagt tgaagtcgag
    11341 tctgttggtg atcgtgactt ggttggaggt atgggaaccg ttgttggggt ttttgaacga
    11401 ttccgataat ttttcggcgc catcttttcc gtgcagatcg taaagtttca cgttgtgggc
    11461 acaatcggta atgagggggc tggtccaccc cttgttggta tgtttgaatc ggatcattcg
    11521 agagcgctga taagtcctgg cttgaggacc catgaccgtc ggggaaaaga ccgttttatt
    11581 atcgacgacg atacatttat gttcatgatg gtggcctatc aattgaaggt tagccggatg
    11641 atttttggag ggaatttgcg ttttgccgga gttgaa
//
LOCUS       DAOSOR010000116        11654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5496_length_11654_cov_4.628071, whole genome shotgun sequence.
ACCESSION   DAOSOR010000116 DAOSOR010000000
VERSION     DAOSOR010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11654
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5496_length_11654_cov_4.628071"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..1167)
                     /locus_tag="PK710_08465"
     CDS             complement(34..1167)
                     /locus_tag="PK710_08465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT09789.1"
                     /translation="MFSTVPLADAGSRSRILFVSKAVAPPFRDGATCLVRDLSQHFQY
                     VTPSVLTVPQAPPLGPRVRAIPFYRKPSRYAPAAIDNVRVLLHLLRDFEHDIWHFVFA
                     PNPRTSQAARLLRKIRRLAVVQTVASRPLRFEATPSLLFGDHVIALSTHTADRLIRHG
                     APAHRISVIPPAVADLTRSENARRKARQQAGIEAEAPLLLYAGDLEFSHGVRRMAEAI
                     PLILREAPETVIAFACRTKTPKAQEQRQRLERHLEPFAGRVRFLGEVDDLPALIASAS
                     ALPFPVDDLYGKVDHPYVVLEAALLETPVIVPKDGPMGEIGDIATMAEGDRQALAKFC
                     VEMVRDGEARRQRGAGLRKAVLRQHDPSEIAGRVEAVYKNILK"
     gene            complement(1192..2232)
                     /locus_tag="PK710_08470"
     CDS             complement(1192..2232)
                     /locus_tag="PK710_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09790.1"
                     /translation="MHIRSVATIITFSTLTITQTAHAQTGGSWGWGTDGGAQGNTYGP
                     GYTTPPVMAPPSSRSNQSSDLEIGTLYGVAATYGVGMGIWIDAETGIDDPGLRFVPPI
                     VLGLAAPVGVYFLDNPPMPRGMPAAIASGMIVGAGEGLGIWSYQFTTMPEDDAWGFRG
                     LSRAMTLGGAVGGGAGFAMAYFQEPSPKSSLLLGSAVVWGSVIGSMVGYGSTEAGLDY
                     GEANDGAALGGLIGYNVGLGAAAGLSAVYVPAWESIGWMWIGGGIGAAAGLPIYLAYA
                     GSDKPAKRGLIIQGITTGLGIVVGGIFSSHISDGYSESTLDQGPKIAEITGFGLMPTQ
                     GGAGLQVMGTLF"
     gene            2427..3485
                     /locus_tag="PK710_08475"
     CDS             2427..3485
                     /locus_tag="PK710_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09791.1"
                     /translation="MVRAMIQFRILFLVAAWSWLACLGCVRGGQASIGVMPGVMNDTG
                     NRSLRRAILRFGLEEFCKELTSRGAPLRMHDDGPVVGRFFARSCNHHELESGDVFVQL
                     DGVGYGWTNATLRMAFTASAAIQYNQDFLMHGSTMYAYFRTRTLVSKDTRVTMVEQGG
                     MIGTAVSALANTAAPGILEQQLQRGFTVIRDTNGTVDFAVGVVEKGKRPVKPFEVRDD
                     DRVTLMNERTEVRGNQLDFLGPFHVDGSNGALFLTMMIDGTSALDVMVVDKNVGDQWL
                     DRFVAQPGVPQPSLPPLVSEIVRQGMRWQKTLPLKKGYYYVVLDNSSVVGLAAPVATG
                     SLPAAALANVVVQVGDAP"
     gene            3482..4552
                     /locus_tag="PK710_08480"
     CDS             3482..4552
                     /locus_tag="PK710_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09792.1"
                     /translation="MNLRLRALWIPIVAWVVSTTIGCPCTDSVINSSPWLRWQIFANF
                     GANNVCSEMTKRGALLSVPVPIAGFPPAVGRFYPQTCQSALNEDRKTLSIWMTGNGYA
                     WTPVTQKMGFECSVAVEYKPDFHKSGDTIYVWFDAVDTPSPQFRIVHVEQAVTNVALS
                     LYPMEWFAGQMAQSFVKEQLAKGFTVIRQDDQDDFSLGRLAVGQRPVHPWQVVGKDRF
                     TFANETTEIRGLQQDFLGPFEIEGKDRALFLRMQVQGPPIDVFVVSKDAGYQWRMQYQ
                     TISPPTPPPAGASIIQSGQTPPSGYFEGWVPVAPGAYYVVLDNSISAGSVQPPLKIPT
                     PVDPSRAVGAKVSYLVQTGDRP"
     gene            4679..6055
                     /gene="lepB"
                     /locus_tag="PK710_08485"
     CDS             4679..6055
                     /gene="lepB"
                     /locus_tag="PK710_08485"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOT09793.1"
                     /translation="MTVNPALRFVFWVTWFFLVPALLAGLAVWLLSPNDAAAAVGPFR
                     LLQSIVADQPVPAAIVLFTLFEMLVWSQRHSLPLAAQVGVGGRRDIPIAVRKRFEQAQ
                     GLLEEAARLERRHHKALQSTVSAEQRSRLEASLTALRESMEAETFDRKRFEESLSNAE
                     RAVDSTLSRWRKGEIREYGESILIAVVVALLLRAFVVEAFKIPSRSMVPTLAVGDHIF
                     VNKFAYGPMIPFTKSRLFNRLPPGRGEVIVFQFPEQPDQDFIKRVIGLPGDRIEAIDG
                     RPRINGWLAPRCRVGEFAYLEGEGELARHHGELFVEYLGERAYPVFVDRTMMSRGERA
                     ACRLDADCAPGQGCAEGVCGELQGPFIVKEGETFMMGDNRMNSHDSRSWFDGRGGGVP
                     FANIRGSALWVWMSFTSGGSVAWDRIGISVMGMPKLPTEFQDNLGARLAECIRNHPGL
                     AAATPPTL"
     gene            6246..6893
                     /locus_tag="PK710_08490"
     CDS             6246..6893
                     /locus_tag="PK710_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09794.1"
                     /translation="MTLPQTWMRRMVGSVAALTLLAMAGCCKDKTEEPTVDPTPTVET
                     TAPTETTEAPAPSNDPEWKTKCPQAERPESGTVTVLRSLHIYKEPNTDSNRESTITPG
                     TWVNLLGAKGTWLCIDYPCGVGKLCPGWIEARYSKRKEPVADAGVDASVTKDAEAPKD
                     AAVKDVAPTDAATKSDASRIRIPRFELIDAGKKTVGGTGGAPGGRPPNPSDVKPK"
     gene            7029..7472
                     /locus_tag="PK710_08495"
     CDS             7029..7472
                     /locus_tag="PK710_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09795.1"
                     /translation="MGPFLRWYVRTLLAVAMLAVAPRGHAESPFKKVEVSTKVARKGI
                     DIPSVFRSEILQRIQGLRTNHPDMNELVLSASLLQLETRRDGHRAHSLCVVSAMLRKP
                     NGALVAVLRGRARAEDGLHAIPDNELAALRAAVKTVMHRLPDAIK"
     gene            complement(7486..7716)
                     /locus_tag="PK710_08500"
     CDS             complement(7486..7716)
                     /locus_tag="PK710_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09796.1"
                     /translation="MKPLIDWTVLSCIAAMSGMSASGLDVDVPLRGQQGWQEAKNADE
                     DSKDEDSKDEDSKDEDSKDGERADEDEGDSDN"
     gene            complement(7738..8520)
                     /locus_tag="PK710_08505"
     CDS             complement(7738..8520)
                     /locus_tag="PK710_08505"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOT09797.1"
                     /translation="MIHPCPLDPWVRPDEQCVALTSDYRLIQRYRGHRYSVDDMLVAR
                     LAASHAAHPRRILDLGCGLGSVLLMLAWRFPRATLVGIEAQPEHVSLARRNVLINGCE
                     DRVTVLQGDLRYDEVFGVEQDGFDLVTATPPYFDPRASTVCSDPQRAHAQWELRGGIE
                     DYALAASKSLSQYGLFVTCAAPTPEHRAPRAFERAGLHPLWHRDVLPKPDKPPFLSLW
                     VAVRDSARCVTEPPLMLRRADGSRTLEHIEIRQWFGIPCSPR"
     gene            complement(8517..9281)
                     /locus_tag="PK710_08510"
     CDS             complement(8517..9281)
                     /locus_tag="PK710_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOT09798.1"
                     /translation="MVDGGNACKMEDSGLIAALVAELERSGLDLRALGLGWARVAPVV
                     AIVPAFGLRALPTVVRAFMGLCLAACIAPAIRADATQLPWAWAIVVAFLHGLPVAIAA
                     AVPLWAATMIGGAVDAVRGASDTVAMPVIEGKPTLFGVPLALLASTIFLTTGGPARVV
                     QALASPSLSPDLSLLRVAWNLSAGIQIAISVAAPVLAASIVVESSTALLARSAAPAQI
                     QSLVAPLRSFGILAVAAFALDRMIRLIALWIGNTPG"
     gene            complement(9256..10209)
                     /locus_tag="PK710_08515"
     CDS             complement(9256..10209)
                     /locus_tag="PK710_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09799.1"
                     /translation="MAKTKTEELIISRYKSNSYEPIVRDYADVSPWPVSLAVGTQTHA
                     YWISAGQLVRREVMSDGTQGSRTVVASDAAPSSPVVAARTTGHVEQDVVLYLGGKVSA
                     QGERSARLFVQGHGTRAISDEAGGATSIALVHLGATRFAILTLDGRLSMSPMHAISLE
                     FDEEGKPELGEDRVVHVAGPAERRSALTGIMVGRGPVALLPLSRTSLEFGLLTLRIGY
                     EDETPRWVDYPNGLDPAPVVAATICGKPMVAFVRPKDASVGSSQVLEVGELSASAELR
                     DHKVIATSKVIPHVSLWGDAASGWIVYVTEQGLRGRRWECL"
     gene            10211..10498
                     /locus_tag="PK710_08520"
     CDS             10211..10498
                     /locus_tag="PK710_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09800.1"
                     /translation="MNRLDNATGAGIPRSISGVSKAVKAEELAGDAEDADDEGAVVGE
                     DDDGGGATETLGEPLVEALATAWEEGGGCRFSQPMSRTHRHVDVIHRVRYA"
     gene            10526..11368
                     /locus_tag="PK710_08525"
     CDS             10526..11368
                     /locus_tag="PK710_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOT09801.1"
                     /translation="MTIDISAVGWKSEPTTMQYGWQQLVLYALGIGAQARELEYLYEG
                     KGPRAYPTFAVVPAISQAFACIERAGVSLSSVVHGGQAVRVLAELPSRGTLRTHGQIV
                     AIHDLKKFAQVMIRTTSELQDGTPVVETEWAIIVRGAGGFGGEPPPRIEHGKVPAGRE
                     PDHRFEQKTLPEQALLYRLSGDINPLHADPEVAARAGFEKGPILHGLATFGFATRAIV
                     ATVCHGNAQRLAFLHGQFRRPVWPGDTLVTEIWRLPDRVVFQTKTQERNEVVLSNAWA
                     DLND"
     gene            complement(11358..>11654)
                     /locus_tag="PK710_08530"
     CDS             complement(11358..>11654)
                     /locus_tag="PK710_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09802.1"
                     /translation="WKSKDDVLVQSVDAQVSALFAHRAYRAVVGVSFAWPRVCVLYGY
                     AFRVDGDEKAAERARDRYALEAFSRLTVNDPHRSPDGVQELPVLDFGDDGEVNR"
BASE COUNT         2520 a         3352 c         3426 g         2356 t
ORIGIN      
        1 cgcaaaagcg caacctcgac cccataaccc caatcatttc aatatattct tgtaaaccgc
       61 ctccacgcgt ccggcgattt cagaagggtc atgctgccgt agcaccgctt ttcgtagtcc
      121 tgccccccgc tgacgtcgcg cctccccgtc gcgcaccatt tccacgcaaa acttcgccaa
      181 cgcttgacga tccccctccg ccatcgtggc gatatcccca atttctccca tgggaccgtc
      241 tttcggcacg atgaccgggg tttcgagcaa agctgcctcc agcaccacgt acgggtgatc
      301 caccttaccg tagaggtcat cgacaggaaa aggcagagcc gaggcggacg ctatcaaagc
      361 ggggagatcg tcgacttctc cgaggaagcg cacgcggccc gcgaacggct cgaggtggcg
      421 ttcgagccgt tgtcgctgct cctgggcttt cggcgttttc gtacggcagg cgaaggctat
      481 taccgtttct ggtgcctccc gcaggataag ggggatagct tcggccatgc gtcgcacgcc
      541 atgggaaaac tccaggtcac ccgcgtacag caagaggggc gcttcggcct cgattccggc
      601 ttgctggcgt gcctttcgtc tcgcgttttc gcttcgggtc aggtccgcga cagcaggagg
      661 aatgaccgag atgcggtggg ctggagcgcc gtgacgaatc agccggtccg ccgtatgcgt
      721 ggacaaagcg atcacgtggt caccaaacag caaggaaggg gtggcctcga agcgcagggg
      781 ccgggaggcg accgtttgca cgacggcaag gcgacggatt tttcgcaaca atcttgccgc
      841 ctgggaggtg cgcggattgg gggcaaagac aaaatgccag atatcgtgtt cgaaatcgcg
      901 cagtaggtgc aaaaggactc ggacgttatc gatagccgcg ggggcgtaac ggctcggttt
      961 gcgataaaag gggatggctc gaacacgagg tccgagcggt ggagcttggg ggactgtcag
     1021 caccgatggt gtaacatatt gaaaatgttg agaaagatca cgaaccaaac aggtagcgcc
     1081 atcgcgaaag ggcggtgcca cggctttgga aacgaaaaga atgcgagagc gggaacccgc
     1141 atccgcgagg ggaaccgtgg aaaacatggt gggtaattcc tgatgagagg attaaaacag
     1201 ggtgcccatg acttgcaagc cagcgcctcc ttgcgtgggc atgaggccaa agccggtgat
     1261 ttccgcgatc ttgggcccct ggtcgagggt ggattcacta tatccatccg agatgtggga
     1321 ggagaagatg cctccgacca caatgccgag gccggtggtg ataccctgga tgatgagtcc
     1381 tcgtttggcg ggtttatcgc tgccggcata agcgagatag atgggcaagc cggctgctgc
     1441 gccaatccca ccgccaatcc acatccagcc aatgctttcc caggcgggga catagacggc
     1501 ggacaagcct gcggcagcgc ctaagcccac gttatacccg atgagcccgc ccaaagcggc
     1561 accatcgttg gcctccccgt aatcgagacc agcttccgtg gagccatatc caaccatgct
     1621 tccgatgacc gagccccaca cgacagcgct tcccaaaagc agggaggatt tgggcgaagg
     1681 ttcttggaag taggccatgg cgaaacctgc tcctcctccg acggcgccgc ccaaggtcat
     1741 ggctcgtgaa agcccacgaa atccccatgc atcatcctcc ggcatggtcg tgaattgata
     1801 ggaccagatg ccgagacctt cccctgcgcc cacgatcatt cccgaggcaa tcgccgcggg
     1861 cataccccgg ggcatggggg gattatcgag gaaatacacg ccaacgggag ccgcgagccc
     1921 aagcacgatc ggcggtacga atcgcaaacc ggggtcatcg attccagttt ccgcatcgat
     1981 ccaaataccc attcccaccc catacgttgc ggctaccccg tacagggtac cgatttccag
     2041 gtcgctcgat tgattcgatc gcgacgatgg gggtgccatc actggcggtg ttgtgtatcc
     2101 gggcccgtag gtattgcctt gtgctcctcc gtccgtgccc cagccccaag acccgccggt
     2161 ctgcgcatgg gccgtctggg taatcgtaag ggtggaaaag gtgattatgg tggcaacgga
     2221 acgtatatgc atggtttgac tccaagacca ggatgtgccg ttggacgaac gaaaaaagca
     2281 aatctttcgc atcaatggct cgtcgggggc aattcgtgga cgggcgtcac ggttcatcga
     2341 ggctatcgca agcggggctg gttcggctag tgaacaaacc tggttcaggc aacgtcgcac
     2401 cggcagttcg aagtatcgta gggttcatgg tacgcgcaat gattcaattc cgtattttat
     2461 ttctggtggc ggcctggtca tggctagcgt gtttgggctg tgttcgaggc ggccaggcat
     2521 cgattggcgt gatgcctggt gtgatgaacg atacgggaaa ccgctcgttg cgtcgcgcta
     2581 ttctgcgttt tgggctggaa gagttttgta aggagctgac atcgcgggga gcgccgttgc
     2641 ggatgcacga cgacggtccg gtggtggggc gatttttcgc ccggtcgtgc aatcatcatg
     2701 aactcgaaag cggtgacgtt ttcgttcagc tcgatggcgt cggatacggt tggacgaacg
     2761 cgactctgcg catggctttt acggcaagcg cggcgattca atacaaccag gacttcttga
     2821 tgcatggctc gaccatgtac gcctatttcc gaacgcgaac gctcgtgtcc aaagacacga
     2881 gagtcaccat ggtcgagcaa ggaggaatga tcggcacggc cgtatcggcg ttagccaaca
     2941 cggccgctcc cggtatcttg gaacaacagc tccaacgagg atttaccgtc attcgcgaca
     3001 cgaacggcac cgtggatttc gctgtcgggg tcgtggaaaa aggaaagcga cctgtcaagc
     3061 ctttcgaggt acgcgacgac gatcgtgtaa cgctgatgaa cgagcgtacg gaggttcgag
     3121 gaaatcaact agactttttg ggaccttttc atgtggatgg gtcgaatggt gccctatttc
     3181 tcaccatgat gatcgacggc acttccgcct tggatgtcat ggtcgtcgat aaaaacgtgg
     3241 gagatcaatg gttggatcgg ttcgtcgcgc agccgggcgt gccccaacct tcgctaccgc
     3301 ccttggtgtc cgaaatcgtc cgccagggaa tgcggtggca gaagacccta ccgctaaaaa
     3361 aaggctatta ttacgtggta ttagacaatt cctcggtggt tggattggcc gctcctgtgg
     3421 ccacgggttc cctgcccgcg gcggcgctcg ccaatgttgt cgtacaagtc ggtgatgcac
     3481 cgtgaacctt cgacttcgtg ctctatggat tccgattgtc gcatgggtgg tatcgacaac
     3541 gatcgggtgt ccgtgtacgg atagtgtcat caacagcagt ccctggcttc gctggcagat
     3601 tttcgcgaat ttcggagcga acaacgtctg cagcgaaatg acaaaaagag gtgctttgtt
     3661 gagtgttccc gtgcccatag cggggtttcc tcccgcggtg ggacggtttt acccgcagac
     3721 gtgccaatcg gcgctcaacg aggatcgaaa aactcttagc atttggatga ccggaaatgg
     3781 atatgcatgg actccggtca cacaaaaaat gggtttcgag tgttccgtgg ccgtagagta
     3841 caaacccgat tttcacaaaa gcggggatac gatttacgta tggtttgatg cggtagacac
     3901 gccgtcgccg cagtttcgga tcgtccatgt ggagcaagcg gtgacgaacg tggcgttgag
     3961 tctgtatccg atggaatggt ttgccgggca gatggcccag tcgttcgtca aagagcaact
     4021 tgccaaagga tttacggtca ttcggcaaga cgatcaggat gatttttcgc tcggtcggtt
     4081 ggccgtggga caacgtccgg tgcatccatg gcaagtagta ggcaaggatc ggttcacgtt
     4141 tgccaacgag accacggaga ttcgggggct ccagcaggac tttttgggcc cctttgagat
     4201 tgaaggcaaa gaccgggccc ttttcctcag gatgcaagta caagggcctc cgatcgatgt
     4261 atttgttgtg tccaaggacg ctggatacca atggaggatg cagtatcaga cgatctcgcc
     4321 gcctacgccg ccccctgcgg gcgcatccat cattcaatcg gggcaaacgc ccccttctgg
     4381 ttatttcgag ggatgggtcc ctgtagctcc cggcgcgtat tatgtcgtgc tcgacaactc
     4441 gatttccgca ggttccgttc agccgccctt gaagattccc accccggtcg atcctagccg
     4501 agccgtcgga gccaaggtga gctacttggt gcaaacgggc gaccggccat gaggcttgag
     4561 accgggcggg tgctcatcct cggaccaggt tcgagccgcc gcgaaatcct cgccacgcgg
     4621 ttgcctcgaa ccgtggccca ggtatacaag gaaggccttc tatttcgagc acatcatcgt
     4681 gaccgtcaat cctgccctcc gtttcgtttt ttgggtaacc tggtttttcc tcgttcccgc
     4741 tttgctcgcc ggcctggccg tctggctctt gtcccccaat gacgcggctg ccgctgtcgg
     4801 tccctttcgg ttgcttcaat cgattgtcgc cgatcagccc gtccccgccg ccatcgtctt
     4861 gttcaccctt ttcgagatgt tggtatggag ccaacgccat agcctaccgc ttgcagccca
     4921 ggtgggtgtg ggtgggcgtc gtgacattcc gatcgctgtc cgcaaacgtt tcgagcaagc
     4981 ccagggactg ctcgaagaag ccgcaagact cgagcgtcga catcataaag cgttgcaatc
     5041 gacggtttct gccgagcagc gatcgcgtct ggaggcgagt ttgaccgcat tgcgcgaatc
     5101 gatggaggcg gagacgttcg atcgaaaacg tttcgaggaa tcgttgagca acgccgagcg
     5161 cgccgtggat tctaccctgt cgcgctggcg aaaaggcgag atccgcgagt atggcgaatc
     5221 gatcctcatc gcggtcgtcg ttgcgctgct gttgcgagcg ttcgtcgtcg aagccttcaa
     5281 aatcccaagc cgttcgatgg tgcctaccct tgcggtggga gaccatatct tcgtcaacaa
     5341 attcgcctac ggtccgatga ttccctttac aaaatcccgg ttgttcaacc gtcttccgcc
     5401 cggccgggga gaagtcatcg tgtttcagtt tccggaacaa ccggatcagg acttcatcaa
     5461 acgcgtgatc gggcttcctg gagaccggat cgaggcgatt gatggtcggc cccggatcaa
     5521 tggatggctt gcgccccgtt gtcgtgtagg ggaattcgca tatttggaag gagaggggga
     5581 gctggctcgc catcacggcg agctattcgt ggaatatctt ggcgagcgtg catacccggt
     5641 ctttgtggat cgaacgatga tgagtcgtgg agaacgtgct gcttgccgcc tcgatgcgga
     5701 ctgcgcgccc ggtcaaggat gcgcggaggg ggtatgtggc gaattgcaag gtccattcat
     5761 cgtgaaagaa ggggaaactt tcatgatggg agacaaccga atgaacagcc acgactcgag
     5821 aagctggttc gacggtagag gcggcggagt acctttcgca aatattcgtg gtagcgcgct
     5881 gtgggtgtgg atgagtttca cgtcgggggg aagcgtcgca tgggatcgca tcgggatatc
     5941 cgtcatggga atgcccaaac tacccactga attccaggat aatctgggtg ctcgcctggc
     6001 cgaatgcatc cgaaatcatc ctgggctggc ggcagccaca cctccaaccc tctgaggttc
     6061 tgtcttgggc tgcccttgcc tcattcaatg gcaagccagc cgggtccgag acttctgcac
     6121 gacccaacgc aaaaacccgg gaagcgttcg gaacaaaatt cccccagtct cacagaatcg
     6181 tacgggaaag atgccaagcc gatgcgggtc gtggtacggt cggcacctct gaaggaggaa
     6241 gctcgatgac tctaccccaa acctggatgc gaaggatggt cggctcggtg gccgctttga
     6301 ccctccttgc catggccggc tgctgcaaag acaagacgga ggagcccacg gtcgacccta
     6361 ctccaaccgt ggaaacgacc gctcccacgg agaccaccga agctccggcg ccctccaatg
     6421 accccgaatg gaaaaccaag tgtccgcagg cagagcgccc cgaatctgga accgtcaccg
     6481 tgttgcgcag tctgcatatc tacaaggagc ccaataccga ttcgaacaga gaaagcacca
     6541 tcaccccagg aacctgggtg aacttgctcg gtgccaaagg tacctggctg tgcatcgatt
     6601 acccctgcgg cgtcggaaaa ctctgccccg gttggatcga agctcgctac tccaaacgga
     6661 aggaacccgt cgcggatgca ggcgtcgatg cttcggtcac caaagacgcc gaagcgccca
     6721 aagatgcagc cgtcaaggat gtcgccccca ccgatgcggc aactaagtcc gacgcttcga
     6781 ggattcgcat tccccgcttc gaactcatcg atgcaggaaa aaagaccgtg ggcggcacgg
     6841 gaggcgcacc aggaggacga ccgccgaatc catccgatgt caaaccgaag taagtgcctt
     6901 tctgccacca cggccttccg tcaatagccc gcgactcttc caggctattt cccacgggcc
     6961 gcctttcccc gcacgatcct atatccgcaa agttggccac atcgagtcgc ttcgttatcg
     7021 ctgacagaat gggtcccttt ttgcgctggt atgtccgaac cctgttggcc gtcgccatgc
     7081 tcgccgttgc gccccgcggt catgccgagt cccccttcaa gaaagtcgag gtaagcacca
     7141 aggtcgctcg caaaggaatc gatatcccat ctgtttttcg ctctgagatc ttgcagcgaa
     7201 tccaaggcct ccgaaccaac cacccggaca tgaacgaact cgttttgtcc gcttccttac
     7261 tccagcttga aacacgtcgc gacggtcacc gcgcgcattc gctctgtgtc gtatccgcca
     7321 tgctacgaaa acccaatggt gcgctcgtcg ccgtactacg tggccgggcc cgcgccgaag
     7381 atggactgca tgccataccc gataacgaac tcgccgccct ccgagctgcc gtgaaaaccg
     7441 tcatgcaccg gcttcccgat gccataaaat aactaataat atcacctagt tatcactatc
     7501 cccctcatcc tcgtctgccc gttccccgtc cttgctgtct tcgtccttgc tgtcttcgtc
     7561 cttgctgtct tcgtccttgc tgtcttcgtc ggcgtttttg gcttcttgcc agccttgttg
     7621 ccccctgagt ggcacatcga cgtccaagcc ggatgcgctc attccgctca tggcagcgat
     7681 gcaggaaagc acggtccaat cgatgagggg tttcatgaga tactactgtg acttgcgtta
     7741 tcgcggcgag caaggaatcc caaaccactg ccggatttcg atatgttcga gcgttcggga
     7801 gccgtcggct cgccgaagca taagcggcgg ttccgtgaca caccgagcgg agtcacggac
     7861 cgcgacccaa agggatagaa atggtggttt atccggtttt ggcaacacgt cgcgatgcca
     7921 gaggggatga agccctgccc gttcaaaggc tcgcggtgct cgatgttccg gcgtcggtgc
     7981 cgcgcatgtc acgaacaatc catattggga taatgacttc gaagcggcca aagcataatc
     8041 ttcgatgccg ccacgcaatt cccattgggc atgggcacgt tggggatcgg agcaaaccgt
     8101 cgacgcccga ggatcgaaat atggcggcgt tgcggtgacg agatcgaagc catcctgctc
     8161 aacaccaaaa acctcatcgt atcgaagatc cccctgcagc acggtaacgc gatcttcaca
     8221 accgttgatc aagacattgc gtcgcgccaa ggacacatgc tcgggttgag cttcgatacc
     8281 aacgagggtg gctcgcggga atcgccaagc cagcatgagc agtacggacc ccaagccgca
     8341 tccaagatcg agaatgcgcc gaggatgagc cgcatggctc gcggcaagtc gcgccacgag
     8401 catatcgtcg acggaatatc gatgcccacg atagcgctgt atcaatcggt aatcgcttgt
     8461 gagcgcaacg cattgttcat cgggacggac ccatggatcg agggggcaag ggtgaatcat
     8521 cctggggtat ttccaatcca tagggcgatg agacggatca ttcggtcgag ggcgaaggcg
     8581 gcgacggcaa ggattccgaa ggagcgtaaa ggagcaacaa gagattgaat ctgtgctggt
     8641 gcagcggatc gggccaacag ggcagtggag ctttccacga cgatggaggc ggcaagcacg
     8701 ggggcggcca cggaaatggc tatttggata cccgcggaga ggttccacgc cactcgcagc
     8761 agggataaat ctggggaaag ggaaggactt gcgagcgctt gcacgacacg ggccgggcct
     8821 cccgtcgtca aaaagatcgt cgatgcaagc agcgccaggg gaactccaaa aagcgtgggt
     8881 ttgccctcga tcacgggcat ggcgacggta tcacttgcgc ctcggacggc atcgacggca
     8941 ccacctatca tggtggcagc ccaaagcgga actgccgcgg caatggcgac cggaagtccg
     9001 tgcaggaacg ccacaacgat tgcccaggcc caaggcaatt gcgttgcatc cgcacgaatg
     9061 gcaggggcga tacaggctgc caaacaaagg cccatgaacg cgcgcaccac cgtcggcaac
     9121 gcccgaagtc caaaagcggg aacgatggca accacgggag ccacgcgtgc ccaaccaagt
     9181 ccaagcgccc gcaagtccag accgcttcgc tccaactccg ctaccagcgc cgcaatgagc
     9241 ccactatcct ccatcttaca agcattccca ccgtcgacca cgaagacctt gctcggtcac
     9301 gtacacaatc caacccgatg ccgcatctcc ccatagagaa acatgtggaa taaccttcga
     9361 agttgcgatg accttgtgat ctcgaagctc ggcgcttgca ctcaattctc ctacttcgag
     9421 aacctgagaa gaacccacgg acgcatcttt cggacgcacg aaagctacca tgggttttcc
     9481 gcatatcgtt gccgcaacca ctggtgcggg gtcgagtcca ttggggtaat caacccatcg
     9541 gggagtttcg tcctcatagc cgattcgcag cgtgagcagg ccgaattcca acgacgttcg
     9601 cgagaggggc aacaaggcta caggtcccct tcccaccatg atgcccgtga gcgcagatcg
     9661 acgttccgcg ggtcccgcca catgaaccac acggtcttcc cccaactcgg gcttgccttc
     9721 ttcgtcgaac tccaacgata tcgcatgcat cgggctcatc gatagccgac cgtcgagcgt
     9781 gagtatcgca aatcttgtcg ctccgagatg caccagagcg atggatgtgg cgcccccagc
     9841 ttcatcgctg atggctcgtg ttccatgccc ctgcacaaat aaccgcgcgg accgctcccc
     9901 ttgcgcgcta acctttccgc ccaaataaag cacgacatcc tgctccacat gacccgtggt
     9961 ccgagcggct accaccggcg aactcggagc cgcatccgaa gccaccaccg ttcgggatcc
    10021 ttgcgtacca tcggacatca cctcccgtcg aaccagttgc cctgctgaaa tccaataggc
    10081 atgggtttgg gttcccaccg ccaaactcac gggccacgga gagacgtcgg catagtccct
    10141 gacaattggt tcataactat ttgatttata acgagatatt atcagttcct cggttttggt
    10201 tttagccacc atgaaccgac tcgacaacgc caccggggcg ggaattccga gatcgatatc
    10261 gggtgtgtcg aaagccgtga aggcggagga gctggccggc gacgctgagg acgccgacga
    10321 tgaaggagcg gtggttgggg aggatgacga tgggggcggt gccacggaaa cgcttgggga
    10381 gccgctggtg gaggcgctgg cgacagcgtg ggaagaaggg ggcgggtgcc gtttttcaca
    10441 acccatgagc agaacccaca ggcacgttga cgtgatccat cgggttcgat acgcgtaggg
    10501 caaacgtttg gctggggaga tgaccatgac aatcgatatt tccgcggtgg gttggaaaag
    10561 cgagcccacc accatgcaat atgggtggca gcagctcgtg ttgtatgcac tgggtattgg
    10621 tgcgcaggca agggagctgg agtatctgta tgagggaaag ggcccccgag cgtatccgac
    10681 gtttgctgtg gttcccgcga tatcccaagc gttcgcatgt atcgaacgcg cgggagtatc
    10741 gctttcgtcg gtcgttcatg gtggtcaagc cgtgcgggtc ctggccgagc taccttctcg
    10801 tggaacctta cgaacccacg gacagattgt ggcgattcat gatttgaaaa aatttgcgca
    10861 agtcatgatt cgcacgacga gtgaactgca ggatgggaca ccggtggtgg aaacggagtg
    10921 ggcgattatc gtgcggggtg ctgggggttt tggtggagaa cctcctccga gaatcgagca
    10981 tggcaaggtt cccgccggtc gagaacccga ccatcggttc gagcaaaaga cacttccaga
    11041 acaagccctt ctttatcgct tgagcgggga tatcaatccg ctccacgccg acccggaggt
    11101 agccgcgcgg gcgggtttcg aaaaaggtcc tattctccat gggttagcaa cattcggctt
    11161 cgccacccgc gccatcgtgg ccaccgtatg ccacggaaat gcccagcggt tggcgttcct
    11221 gcacggtcaa tttcgtcggc ctgtatggcc cggtgacacc cttgtcaccg aaatctggcg
    11281 actaccagac cgcgtagtct ttcaaaccaa aacgcaagag cgaaacgaag tcgtcctatc
    11341 caacgcttgg gccgatctca acgattgacc tccccatcgt cgccaaaatc aaggacaggc
    11401 agttcctgca caccgtcggg agaacgatgg ggatcattga cggtcaagcg tgaaaacgct
    11461 tccagggcgt agcggtcgcg tgctcgctcg gccgctttct catcgccatc gacacgaaag
    11521 gcatagccat agagcacgca gactctcggc caggcaaaag aaacaccgac cacggcgcgg
    11581 taggcgcgat gggcgaatag cgctgacact tgtgcgtcca ccgattggac gagcacatca
    11641 tctttggatt tcca
//
LOCUS       DAOSOR010000117        11638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5507_length_11638_cov_3.978589, whole genome shotgun sequence.
ACCESSION   DAOSOR010000117 DAOSOR010000000
VERSION     DAOSOR010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11638
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5507_length_11638_cov_3.978589"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(279..1751)
                     /locus_tag="PK710_08535"
     CDS             complement(279..1751)
                     /locus_tag="PK710_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HOT09803.1"
                     /translation="MAPMLTAISALMQSSGVTFGTSGARGLVTAMTDRVCYAYTVAFL
                     QHLEQHGRCQPGAQVAIAGDLRSSTDRIMRAVACAVSDRGYMPVSCGHIPSPAVASFG
                     LRHRIAAIMVTGSHIPDDRNGIKYNAPEGEILKSDEESIRNQSVLLPERFDAQGMLRD
                     GTLPATQRQAARDYVHRYVDVFPREMLAGKRLGLYAHSAVGRDIIHEILEALGAHVTR
                     LADSDVFIPVDTEAIRPEDIALAASWAKEYGFDALLSTDGDSDRPLIADEHGRWLRGD
                     VAGILCAKQLGADVVVTPVSSNSAVEECKAFSQVIRTRIGSPFVIEAMLRACQGSAKR
                     VVGYEANGGFLTSSPISAPRGLLPALPTRDAVIVHLSLLWASASSNRSLSAIVKDLPA
                     RFTWSDRLQNVPTQLSQGRLEELRRDGLPAIARQFEVFGPLSTVDWTDGMRMTFASGA
                     VVHIRPSGNAPELRCYTEGASEDQARQWNAVALEIVGQWK"
     gene            1842..2585
                     /locus_tag="PK710_08540"
     CDS             1842..2585
                     /locus_tag="PK710_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09804.1"
                     /translation="MVARKLQWSTWLLAIVVVGAAGCGGGSEEGDSVTSDAGADVSAD
                     GSGGSGGSGGSGGASGGSSGSGGSAGAAGGAAGTAGTGGGGIGGSSGSGGVAGAAGSS
                     GASGAAGSAGSGGAGPTCPTSQPKNNTDCTGAGLVCTYGDYNACASKASGIAPPVQRN
                     NRRTTVPATSLVHHAPMETNNAAAPTLVGIAIHAQRRNPRTTMFVMIPDCNAFTDRWN
                     AGVATKDDGGAKDAPPHSRLRKRDVVIKA"
     gene            2735..3094
                     /locus_tag="PK710_08545"
     CDS             2735..3094
                     /locus_tag="PK710_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09805.1"
                     /translation="MSNWRCSGCPNQEPANKSQCSQQGLDCTYGATQCNCSFGTWNCA
                     TCPSQQPANKSQCSSVGLKCEYGDTDCDCKMTGWDCVTCPSAKPQNNSACSEQGAQCT
                     YGSSTCSCFGGAWHCWG"
     gene            3166..4026
                     /locus_tag="PK710_08550"
     CDS             3166..4026
                     /locus_tag="PK710_08550"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HOT09806.1"
                     /translation="MIGSRWFVAWGLVLGCLSCGTNESRTNIRNGGDAALDAEGNGEA
                     DATGTAGQAGYAGQASAGGAGGNAGHQEGGVVPPPAGGAVVFPGVFNARNEGGYLTVS
                     GQRIKHQVLIRSGHLADLGASGCQRFEELSIRSVLDLRAADDAASNPDAACVTQHASY
                     TLVDLPKILPPSVDAYRQTLDATEPKLSIIFSKLSEDGALPAIVHCVIGRDRASLVSA
                     VLLLALGVSQEDVKKDFVDNQDDTVHVEAAWLSPLLDRVDGAGGIEPYLLSHGVTQAM
                     IERFRVQALE"
     gene            complement(4054..5304)
                     /locus_tag="PK710_08555"
     CDS             complement(4054..5304)
                     /locus_tag="PK710_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09807.1"
                     /translation="MCPRLLVLVSTFIIPWGCAIDFDAPFAQSTTEHRDGGWEASSGG
                     SGGKTIVGGSGGAAEGGSGGTSAGGTGGSGAGGAGGTSGGGTGGSGAGGAGGSAAGGF
                     GGSGGSGDEICNNGKDDNNDGLVDCLDPHCKSLGFSCLPTPPSPWSGPIQFNRGTLFT
                     CDEPWPVPSVSGKIGLHFDGQNSCPTCGCAPSTNSQCGLDVTVYKQEGCMGEEGDSKF
                     YLGGSCVNVGMQMFGPHSVRPISDMHVMGGSCDVQTQGEVKLPPAQWQEEAWTCQAQM
                     GGGCQASEVCSPPAQGSRICVTRSGVYDQACPADYKDSSWVVYEGIKDTRSCASCSCS
                     PATGNCSGGQMSVHDPSGTCTDAGEVLKTGQCTNLPIHPTMGTFTLKMIKPGGFNPTC
                     TPSGGGLEGDVTATNPITVCCRNP"
     gene            complement(5424..6017)
                     /locus_tag="PK710_08560"
     CDS             complement(5424..6017)
                     /locus_tag="PK710_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09808.1"
                     /translation="MNHSFLCRLMTFVVFGLTAGTAGCCKDKSDDAQPIPFPEPKPDE
                     TKVLTPSQETAQPASTPTPTTASTNNYQSNGFPEVMPSQRTPVPTVSEWSRAPVITTK
                     KFPDGCHMKFVREWLKINCSKDTDTATPLRISSITGFGSEGADYFKFEKPGKVLDIIV
                     RTKDGKNGSAIYVMDRRSYRVGYHWPYRAPFPSTIFE"
     gene            6207..7874
                     /locus_tag="PK710_08565"
     CDS             6207..7874
                     /locus_tag="PK710_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09809.1"
                     /translation="MRLLSANGRAFPVVFGVEEGSHFRVVAIALRFSDRCRRACKRFE
                     KLGVGSSFMLRFARGHCMLRKARTSFSLGPWSWVLVSSLLGCASGEDADRSFANGENG
                     GKDGSSDSSEQADADTETSFPSDFGMPCQSDEDCSSGLCVEGADGTVCSAFCSDSCPK
                     DYDCLSYEGLQGPHDHVCIPQISRLCRPCKSDTDCQRHGVVQDASRCILQGDFGSFCG
                     VSCSGTCPDSYACDEVKLASGVSVFQCVPEGGRSCECRPSWAALGLETTCTNTNSYGV
                     CTGSRTCDENGLTPCSAPTPSAEVCDGTDNDCDGHVDNGTCSDNLDCTEDVCDGKGQC
                     SNPIQSSSCLIDGECKNSGDLNPTNSCQSCKPSSSQTAWTVLTGSCDDGDVCTVQDTC
                     VEGVCVGQVQQDSHEPNDDLNSCTSFNAVSDCDSYPKGSLEATLYGAGDEDWFTYTVS
                     DNAFCLIYPRVSLTVPTGSNFDLCAYYTCKNGGTLSLKCTHGTLSTGYGMSGCCSNNP
                     GDAAENVRLTPTCSGGGFDDSGTVYVRVFNAEPTFTCAPYTLQYGDD"
     gene            8036..8599
                     /locus_tag="PK710_08570"
     CDS             8036..8599
                     /locus_tag="PK710_08570"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09810.1"
                     /translation="MADPSFVTPCHALLRTRPAVDDPPCLPQLLIESGLIEAESLDEA
                     IRIAANSSQRLLDVLLSHDIVDERVVAAMLSYELSMPWVDPALIHHDRCAAHALPRWV
                     AESLHVLPIRYQQRGEGIFLLVATDDPTDHEAMVACSVWAGMPIRPLVACTSAIHKAM
                     GRWFDEVQVGEIIDENEDALPQTVAVA"
     gene            complement(8600..9487)
                     /locus_tag="PK710_08575"
     CDS             complement(8600..9487)
                     /locus_tag="PK710_08575"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HOT09811.1"
                     /translation="MQATQATIRPRRSVLYVPGSNLRALEKARTLPADGLIFDLEDSV
                     APEVKETARQNVLRAIEQGGFGKRELFLRVNGLNTPWGYDDLWAAAQSKADAVVLPKV
                     DGPEVVKQAVSVLWEAKAPEQLSVWCMMETARGMLHAESIANASTRLGGLVMGTSDLA
                     HELHAAHTPLRLPMLTALGLCLLAARAAGIPILDGVYLDLADDKGFEASCQQGMELGF
                     DGKTLIHPRTIEIANRVFAPSNEQVQRAKAIIVAFEEASAAGKGVVVVEGKLVENLHV
                     ENAKRIVKLSEAIESTKIG"
     gene            complement(9663..11597)
                     /locus_tag="PK710_08580"
     CDS             complement(9663..11597)
                     /locus_tag="PK710_08580"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HOT09812.1"
                     /translation="MRANVDDAFRTWAESQPDCWEQYRARSGTMTDGYSGYGIGSMQG
                     SSRGEAGAPLSGGHSVPKVRLGSSTVNGRPAPPSPRRAKSASTTNLQVAGVDEADFVK
                     TDGQYVYVVSNGKLRIIEAMNPRVLSTTRLDGQAREMFITENRAVVYTSSRGQTRRCT
                     YGYDCQFAADGTSTMITVYDISNRSAPRSVRRIELSGSLLTARRIGNAVHTVVADADR
                     TEQLLYWPRDLEMCGTLEASVRAKFAALKRENERIIRATGVGTPTLRENGVETALCIG
                     YETRLRHENAYTTLVSIDLLQDQGPAATSTVATRPGAVYASVDALYLASSQHKPHSAG
                     RWYSFYSSSEEVTEIHKFRIGSSPGDTRYLGSGIVPGHVLNQFAMDEWNGNLRIATTQ
                     GRVPNPNVRSTVSVLSQSGENLVRVGRVTNIAPEEDIRAVRFDGDRGYIVTFKKTDPL
                     FVLDLHDPLHPRIVGELKIPGFSTYIHRIGRNHLLSIGFDADDLDEYALFNGVMLQLF
                     DVTDSTKPRLIHKETIGTRGTSSEAIVNHLAFNYLEDRKLLAFPMTICEGGGRGRYGT
                     LTFSGLLVYEVDIQKGFKRLGGIDHGTANASCGTWWSNATSMVKRSLFMDDLVYSIAL
                     DRIKVQRLGNFRTDVASIDL"
BASE COUNT         2470 a         3330 c         3223 g         2615 t
ORIGIN      
        1 caagggcaac aaagcaagcg acaacaatga ccatcaccgc accatcgaca cgtcccacac
       61 gctgcctatt tgcgaatcca tgccggatgg ggaagacggt atggttgata ccgatgtcgt
      121 caaatttcgg acacaaaaat catcctttcg acgatggcgg gccgccggtc gtattcgaaa
      181 atccataagc gcccgacata ggaagtccgc atgcccttgg ctgttctgga caccatcctt
      241 gtcgggcgac ccaatttgtg gaagccgaaa cgaatacgtt acttccactg tccaacgatt
      301 tccagggcga cggcattcca ctgtcgagcc tggtcctccg acgccccttc cgtgtagcac
      361 cgtagttcag gggcatttcc cgaggggcga atatgaacga ccgcgccgct ggcaaacgtc
      421 atgcgcattc catccgtcca gtcgaccgtc gacaaggggc caaatacctc gaattggcgt
      481 gcgatggcgg gcaatccatc gcgacgcaat tcctccaaac gtccttggct cagttgtgtg
      541 gggacgtttt gcaagcggtc gctccacgtg aaacgcgccg gcaagtcctt cacgatcgcg
      601 gaaaggctgc ggttcgagga ggcggaggcc caaaggaggg aaagatgaac gatgacggcg
      661 tcgcgggtgg gcaaagccgg cagaaggcca cggggggcag aaatgggcga actcgtgaga
      721 aatccaccgt tggcctcgta gcccaccacg cgtttcgcgc tgccctggca tgctcgtagc
      781 atggcttcga tgacgaaggg cgaaccgatt cgtgtacgaa tcacttggga aaatgctttg
      841 cattcctcca cggccgaatt gctgctcaca ggagtcacca ccacatccgc cccgagttgt
      901 ttggcgcaaa ggattcccgc gacgtctccg cgaagccagc gaccgtgttc gtcggcaatc
      961 aaaggacgat cgctatcccc atcggtggat aaaagggcgt cgaaaccata ttctttggcc
     1021 cacgatgccg ccagggcaat gtcttcgggc cggatcgctt cggtatcgac gggaatgaaa
     1081 acgtccgagt ccgccaatct cgtcacgtgc gcgccaagag cttcgaggat ctcgtgaatg
     1141 atgtcacgcc ccaccgcgga gtgggcgtac aggccaagcc gtttgcccgc gagcatttct
     1201 cttggaaata cgtcgacata tcggtggaca tagtcccgag cggcttggcg ctgcgtggct
     1261 ggcagcgtgc cgtcgcgaag catcccttgc gcgtcaaatc gttcaggaag caaaacgctc
     1321 tggtttcgaa tggattcttc atcggatttg agaatctctc cttccggagc gttgtacttg
     1381 atcccattgc gatcgtcggg aatatgactt cctgtcacca tgatcgcggc tatgcggtgt
     1441 cgaagaccga aagaagcgac agcgggtgaa gggatatgac cacaagagac gggcatgtat
     1501 ccccgatccg acacggcaca ggcaacggca cgcatgatgc ggtcggtgct ggaacgcaga
     1561 tcgcctgcga tggccacttg cgccccgggc tggcaacgac cgtgttgctc gagatgttga
     1621 aggaaggcga ccgtgtaggc gtaacaaaca cggtctgtca ttgccgtcac cagtcctcgg
     1681 gctccgctgg tgccgaacgt cacaccgctg gactgcatca acgcggatat cgctgtcagc
     1741 atgggggcca ttggtagcac gaacttattg tatccaaggg attcgtttgc atttttgttc
     1801 ttatggcggg acactgtagc agtctgtgga ggaggtgatg catggttgct cgaaaacttc
     1861 aatggagtac ctggttattg gcgatcgtcg ttgtcggtgc ggcaggctgc ggcggcggct
     1921 ctgaggaagg agactccgtc actagcgacg cgggggcgga tgtatccgcg gacggctccg
     1981 gtggttcggg aggttcgggc ggttcgggtg gtgccagcgg tggatcgtcg ggaagcggtg
     2041 gttccgcggg agccgcgggc ggtgcggccg gtaccgcagg aaccggtgga ggaggtattg
     2101 gcggttcgtc aggaagtggc ggagtagccg gcgccgctgg ctcttcaggt gcttcgggcg
     2161 cggcaggctc ggcaggaagc ggaggcgccg gcccaacgtg tcccacgagc caacccaaaa
     2221 acaacaccga ttgcactggt gcgggtttgg tctgcaccta cggcgattac aatgcttgtg
     2281 cttccaaggc aagtgggatt gctccgcctg tccaacgcaa caaccggcgg acaacagttc
     2341 ctgcaacgag cttggtgcat catgctccta tggaaacgaa caatgccgct gcaccaacgc
     2401 tggttgggat tgcaatacat gcccaacgac ggaacccgcg aacaacgatg tttgtaatga
     2461 taccggattg caatgcgttt acggacagat ggaatgccgg tgtagcaacc aaggacgatg
     2521 gcggtgcaaa ggatgccccg ccacacagcc ggctccgaaa gagggatgta gtgatcaagg
     2581 cctgacctgc gactatggaa ccacgacttg ctcctgcaac tacgggggat gggtatgcgg
     2641 agattgcccc gcacaagagc ctgaccattc atcgtcttgc accgacaaag gcctgtcttg
     2701 tacgtacggg acgcgaacgt gcgagtgtag tatgatgagc aactggcggt gttcgggctg
     2761 tcccaatcag gagcccgcga acaagagcca atgttcgcag caagggctgg actgcaccta
     2821 cggagccacg caatgcaatt gttcgtttgg gacgtggaat tgcgccacct gcccctccca
     2881 gcaacctgcg aacaagagtc agtgttcgag cgttggcttg aagtgcgagt acggggatac
     2941 ggattgtgat tgcaaaatga ctggttggga ttgcgtgacc tgtccttccg ccaagccgca
     3001 gaataacagc gcctgctctg aacaaggggc ccagtgtacc tacggttcct cgacatgctc
     3061 ctgctttgga ggcgcgtggc attgctgggg ctgaaaccgc cttccagtaa ccatttcgta
     3121 gaagtaagct aacctattca tcctcgagac cgtggagcga tgttcatgat cggcagtcga
     3181 tggtttgtcg cgtggggtct tgttctgggt tgcctgtcct gtggaaccaa tgaatctcgc
     3241 accaatattc gcaatggtgg cgatgccgcc ctcgatgccg aaggcaatgg tgaggccgac
     3301 gcgaccggca cggcaggtca agctgggtac gccggtcaag cctccgcagg aggagccggt
     3361 ggaaatgccg gtcaccagga agggggtgtc gtccccccgc ccgcaggagg agccgttgtt
     3421 tttcctggcg ttttcaacgc acgcaacgaa gggggctatc tgaccgtttc cggacagcga
     3481 atcaaacacc aagtgctcat tcgctcgggc caccttgcag atctaggtgc aagcggttgc
     3541 cagcgcttcg aggaactcag cattcgctcc gttctcgatc ttcgtgcggc cgatgacgct
     3601 gcaagcaacc cggatgcagc ttgcgtaacc caacacgcct cgtatacctt ggtagaccta
     3661 cccaaaatcc ttcccccatc cgtcgatgca tatcgacaaa cgctcgatgc cacggagccg
     3721 aaattatcta ttattttcag taagttatca gaagatggcg ctcttcccgc catcgttcat
     3781 tgcgtcattg gtagagaccg agcaagcctt gtctctgctg ttcttcttct cgccctcggc
     3841 gtatcacaag aagacgtgaa aaaagacttc gtcgacaacc aagacgatac ggttcacgta
     3901 gaggctgcct ggttgagccc gctgctcgat cgtgtggatg gtgcaggggg gatcgagcca
     3961 tatttattat cccacggggt aacgcaagcc atgatcgagc gctttcgagt gcaagctctc
     4021 gaatgaacca ggaaggacga gaccgcgttc atctcatggg tttcggcaac aaacggtgat
     4081 tggattggtg gcggtaacat ctccttccaa ccctccgcca cttggggtgc aagttggatt
     4141 gaaccctccc ggtttgatca ttttgagcgt gaacgttccc atggtgggat ggatcggcag
     4201 gttcgtgcat tgtccggttt tcaagacttc cccggcatca gtgcacgttc ccgagggatc
     4261 gtggacggac atttgccctc cgctgcaatt gcccgtggcg ggagagcaag agcaggaagc
     4321 gcatgaacgc gtgtccttga ttccttcgta tacgacccaa ctcgaatcct tgtagtcggc
     4381 tgggcaggct tgatcataca ctccggaacg agtgacgcag atccgggacc cttgagcggg
     4441 gggggaacag acttcggagg cctgacatcc tcctcccatc tgcgcctggc aagtccaggc
     4501 ttcttcttgc cattgggcag gcggaagctt cacctcgcct tgggtttgaa catcgcaact
     4561 tccacccatc acgtgcatgt cagagatggg gcgtacggag tgaggaccga acatctgcat
     4621 cccaacgttg acgcaactgc cacccaaata aaacttcgaa tccccttctt cgcccataca
     4681 tccctcttgt ttgtagacgg tgacatccag tccgcattgt gagttcgtcg atggggcgca
     4741 cccacaagtg gggcatgagt tttgtccatc gaagtgcaag cctatctttc cgctgacact
     4801 cggaacaggc caaggctcat cacaggtgaa aagcgtacca cggttgaact gtatcgggcc
     4861 cgaccagggg gaagggggcg tgggaagaca cgaaaatccg aggctcttac aatggggatc
     4921 gagacaatcc acgagcccgt cgttgttgtc gtctttaccg ttgttgcata tctcatcgcc
     4981 acttcccccc gatccaccga agccccctgc tgccgatcca cccgcgcctc ctgctcctga
     5041 ccctccggtg cctcctccac tcgttccgcc cgcgcctccc gctcccgagc ccccagtgcc
     5101 tcctgcactc gttcctccag acccaccttc cgccgcgcca ccagagccac ccacaattgt
     5161 ttttcctccc gatcctcccg acgacgcttc ccatccaccg tcgcgatgct cggtcgtgga
     5221 ttgagcgaac ggagcatcga agtcgatggc gcaaccccac gggatgatga aggtggaaac
     5281 cagaacgagt aggcgggggc acatgggatt cctcacgaca atacgttcaa gagcgcgtcc
     5341 tttgccatcg cgctcgacct gcttctgatc ctatcaaatc cggctagaca cgccagcaca
     5401 cgtgcgttcc gatagcccaa cgattactca aatatcgtgc ttggaaaagg tgcacgataa
     5461 ggccaatggt aacccacacg gtaacttctg cgatccatga cgtagatggc cgaaccgttt
     5521 ttcccatcct tggtccgcac aatgatatcg aggacttttc ccggtttttc gaacttgaaa
     5581 tagtcggccc cttccgaacc gaaacccgtg atactgctga ttcgcaacgg agttgccgta
     5641 tccgtatcct tggaacaatt gatcttcaac cactcgcgta cgaacttcat gtgacatccg
     5701 tcaggaaatt ttttcgtcgt gatgacgggg gctcgtgacc attcggaaac ggtcggcacc
     5761 ggcgttcgct gggatggcat cacctcggga aaaccgttgg attgatagtt gttcgtcgat
     5821 gctgtcgtgg gagtgggcgt cgacgccggt tgtgctgtct cttggctcgg agtcaacact
     5881 ttggtctcgt ccggtttggg ttcggggaaa ggaatcggtt gcgcatcatc ggatttgtct
     5941 ttgcaacacc ccgccgtgcc tgcggtgaga ccgaaaacaa cgaacgtcat aaggcgacat
     6001 agaaaagaat ggttcattcg acgtatccct ttcgcatcca aagacccgat taatcatcat
     6061 atcgtttcct gtttgttcgg agcaatgcga tccgcttaat ccgctttttt tcattcgtga
     6121 ccctgcgttg gcaacgctga cattcccgtt ggccatggtg gcggtcaaac ggtcttcgag
     6181 gttcttgcca tcgatgggtc tctctcatga gattgctttc cgcgaacggt cgcgcttttc
     6241 ctgtcgtatt tggggtcgag gaggggagcc attttcgagt cgtcgcgatt gctttgcgct
     6301 tttccgatcg ttgccgccga gcgtgcaagc gtttcgagaa gctgggagtc gggtcgtctt
     6361 tcatgctacg gtttgccaga ggtcattgca tgctccgaaa agccaggaca tctttctccc
     6421 tcggcccctg gtcgtgggtt cttgtatcgt cactgcttgg atgtgcttcc ggagaagatg
     6481 cggatcgctc ttttgccaat ggtgaaaatg gcggaaaaga cggctcctcc gattcctccg
     6541 aacaagcgga tgctgacacc gagacctctt ttccttcgga ctttggaatg ccgtgccaga
     6601 gcgatgaaga ttgctcatct gggttatgtg tcgaaggtgc agatgggact gtatgttccg
     6661 ctttttgttc ggactcctgc ccaaaagact acgattgcct ttcttacgag gggttgcagg
     6721 ggcctcacga tcacgtgtgc atcccgcaaa tctcgcgact gtgccgcccg tgcaaaagcg
     6781 atacggactg ccagcgtcat ggcgtcgtac aggatgcttc ccgatgcatt cttcaagggg
     6841 atttcggctc gttttgcgga gtatcgtgct cggggacctg tcctgattcg tacgcttgcg
     6901 atgaagtgaa actcgcctcc ggcgtgagcg tattccaatg cgttccagaa gggggccggt
     6961 cttgtgaatg taggccttcg tgggccgcgc tgggattgga aacaacgtgt accaacacca
     7021 attcatacgg tgtttgcaca ggctcgcgca cttgcgatga gaatggcctc accccttgtt
     7081 ccgcccccac accaagcgcc gaagtctgcg atggcaccga caatgactgc gatggtcacg
     7141 tcgacaacgg tacgtgtagt gacaatctcg attgcaccga ggatgtctgc gacggcaaag
     7201 gccagtgctc caatcccatc caatcatcct cctgcttgat agatggcgaa tgcaagaatt
     7261 ccggggacct caaccccacc aactcctgcc aatcctgcaa accatcgtct tcgcaaacgg
     7321 cgtggacggt cttgactggc tcttgcgatg atggcgacgt ctgtaccgtg caggatactt
     7381 gtgtggaagg ggtatgtgtc gggcaggtgc agcaagattc ccatgaaccc aacgatgact
     7441 tgaattcatg tacctctttt aacgccgtct ccgattgcga tagttatccc aagggcagct
     7501 tggaagctac gttatatggt gcaggagacg aggattggtt cacctatacg gtatccgata
     7561 acgccttctg tctcatctat cctcgtgtat ctcttaccgt gcccacgggc tccaacttcg
     7621 atctatgcgc ctactacaca tgcaaaaacg gtggcaccct atccctgaag tgtacgcacg
     7681 gcactctgtc cacaggctat ggaatgagtg gctgctgttc caataacccg ggcgacgcag
     7741 cagaaaatgt ccgtctcaca cccacttgct cgggcggcgg cttcgacgat tcgggaaccg
     7801 tttacgttcg cgtattcaac gcagagccta cgtttacctg cgctccgtac acactgcaat
     7861 atggcgacga ttgacgtgac ctaatagatc ctctccagag atccaatcga tccctttcgg
     7921 atacccaaga acaccatttc tccagcaccg atacggttta ataaccaagc atgccgtggt
     7981 ttcgttggca tgtatcgtgc tccgtgtggg ccgggactgt agaggagaac cacggatggc
     8041 cgacccctca ttcgtcaccc cctgtcatgc tttgcttcgg actcgacctg ccgtagacga
     8101 tcctccttgc ttgccacagt tgcttattga aagtggcttg atcgaagcgg agtccctcga
     8161 cgaagccatt cgcatcgctg ccaatagctc acaacgtctg ctcgatgtcc tgttgagtca
     8221 tgacatcgtg gatgaacgcg tcgttgccgc catgctttcc tacgaattga gcatgccatg
     8281 ggtcgatcca gccttgattc accacgatcg ctgcgcggcg cacgctcttc ctcggtgggt
     8341 tgcagagagt cttcacgtgt tacccattcg ttaccaacaa cgtggtgaag gaattttttt
     8401 gcttgtcgct accgatgatc cgacggatca cgaggccatg gtcgcgtgct cggtgtgggc
     8461 cgggatgccc attcgtccgt tggtggcttg caccagcgcc atccacaagg cgatgggtcg
     8521 ttggttcgac gaagtgcaag taggcgaaat aattgatgaa aacgaagacg cgttgccgca
     8581 gaccgtagcc gtggcgtgat catccgatct tcgtagactc gattgcttcc gacaatttca
     8641 cgattcgttt tgcattttcg acgtgaaggt tctccacgag ttttccctcc acgaccacca
     8701 ctccttttcc ggcggcggac gcctcctcga acgcgacaat gatggctttg gctcgttgca
     8761 cctgttcgtt cgagggggcg aacacgcggt tcgctatctc gatggtcctg ggatgaatca
     8821 acgttttgcc atcaaatcca agctccatgc cttgttggca tgaagcttcg aaacccttgt
     8881 catcggcaag atcgagataa acaccgtcga gaatgggaat gcccgccgcc cgcgcagcga
     8941 gaagacacaa gccaagtgcc gtcagcatcg gtaatcgcag gggtgtatgt gcggcatgaa
     9001 gctcatgagc aaggtcactc gtgcccatca ccaaaccacc caatcgcgta ctcgcattgg
     9061 cgatcgactc cgcatgcagc atgccccgcg ccgtctccat catgcaccat accgatagct
     9121 gctccggcgc ctttgcttcc cacaaaacgg agacagcttg cttcaccacc tccggaccat
     9181 cgactttcgg aagaaccacc gcatccgctt ttgattgtgc cgcggcccaa agatcgtcgt
     9241 acccccacgg cgtattcaac ccattgacac gaaggaacag ttcccgcttt ccaaagccgc
     9301 cttgctcgat ggcgcgaagc acgttctgcc gggccgtttc tttgacttcc ggcgcaaccg
     9361 aatcctccaa gtcgaaaatc aatccatcgg caggcagcgt ccgagctttt tccaaggcac
     9421 gaagattcga acccggcaca tacagaacgc ttcgtcgagg acggatcgtt gcttgcgttg
     9481 cttgcataag tatcaccctt tgcctgtttc ccgccgcgag tcatggcctg tgatcatcgc
     9541 ttccccctcg actcaccaag accatcgacg cgatcccatc tgggtggttt gcggatatgg
     9601 gattgtaaac accttttggt aacgagaaga agtacgggat agcgtaaccc gcagtgcggt
     9661 tgctacagat cgatactagc tacatccgtt cggaaattgc ctagtcgctg cactttgatt
     9721 cgatccaacg cgatcgaata caccaaatcg tccataaaca ggcttcgttt taccatgctc
     9781 gtggcattgg accaccatgt tccgcaactg gcattggctg tgccgtggtc gattccgccc
     9841 agacgcttga atcctttttg aatgtccact tcatacacga gcaagccgct gaacgtaagc
     9901 gtgccatatc ttccccgccc tcctccttcg caaatcgtca tgggaaaggc gagaagcttg
     9961 cggtcttcca ggtagttgaa ggccagatgg ttgacgatgg cttctgaact cgttccccgc
    10021 gttccgatcg tttccttatg tatcagccga ggtttcgtgg aatcggtcac atcgaagagt
    10081 tgaagcatca ccccattgaa caacgcatac tcatctaagt cgtccgcatc gaatcctatc
    10141 gacagcagat ggttccggcc gattcggtgg atatacgtcg aaaagccggg aatcttgagt
    10201 tcaccgacga tcctgggatg caaaggatcg tgcaggtcga ggacgaaaag cggatcggtt
    10261 ttcttgaacg tgacgatata tccgcgatcg ccatcaaaac ggacggcacg gatgtcctct
    10321 tcaggagcga tattggtcac gcgtcccacc cgcacaaggt tttcgcccga ttgggaaagc
    10381 accgatactg tgcttcgaac gttcggattg ggtacccgac cttgcgtcgt ggcaatgcgg
    10441 agattcccat tccactcgtc catcgcaaac tggttcaaca catgaccagg aacgataccg
    10501 ctgccaaggt atcgggtgtc accaggagag gaaccgatcc gaaacttatg gatctccgtc
    10561 acctcttcgc tggacgaata aaagctgtac cagcgccccg cagagtgagg cttatgttgc
    10621 gaagaagcaa ggtaaagtgc gtcgacagaa gcatataccg cgccaggacg agttgcgacc
    10681 gtagacgttg cagcgggacc ttgatcttgc aacaaatcaa tcgatacgag ggtggtatac
    10741 gcgttctcat gacggaggcg cgtttcgtat ccaatgcata acgccgtttc gacaccgttt
    10801 tcacgcaagg tgggtgtgcc aactcccgtc gcgcggatga ttcgctcgtt ttcgcgcttt
    10861 agggcggcaa acttcgcgcg taccgacgcc tccagcgttc cgcacatctc caggtctctc
    10921 ggccaatata agagttgctc cgttcgatca gcgtccgcaa ccaccgtatg aaccgcgttg
    10981 ccgattcttc gagccgtgag aagagagcca gagagttcaa ttcggcgcac cgaacgcggc
    11041 gccgatcgat tcgaaatatc atataccgtg atcatcgtcg acgtgccgtc cgccgcaaat
    11101 tgacagtcgt agccgtaggt gcatcgtcga gtctgcccac gactcgatgt atatacgacc
    11161 gctcggtttt ccgtgatgaa catctcgcgc gcttggccgt cgagacgagt cgtggacaat
    11221 acacgcgggt tcatcgcttc gatgatgcgt aacttgccat tggagacgac atacacatac
    11281 tgcccatccg ttttcacgaa atctgcttcg tcaacacccg caacttgcag attggtcgtc
    11341 gatgcacttt tcgcacgacg gggagaaggt ggtgcggggc gaccattgac ggtggaacta
    11401 cctagcctga cctttggcac cgaatggcct ccggagagag gagcgcctgc ttcgccacgg
    11461 ctggagcctt gcatcgagcc aattccgtac ccgctgtatc cgtctgtcat tgtgccgctg
    11521 cgtgctcgat actgctccca gcagtccggt tgactctccg cccaggttcg aaaagcatca
    11581 tccacgttcg cgcgcatgtc ggcgattcaa ccggactgct gggagcagta tcgagcac
//
LOCUS       DAOSOR010000118        11631 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5518_length_11631_cov_4.609191, whole genome shotgun sequence.
ACCESSION   DAOSOR010000118 DAOSOR010000000
VERSION     DAOSOR010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11631
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5518_length_11631_cov_4.609191"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(346..639)
                     /locus_tag="PK710_08585"
     CDS             complement(346..639)
                     /locus_tag="PK710_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09813.1"
                     /translation="MQRIKQLEEKLASLSAHNATLQEAVQRMQSGFRQHRSEVVSADQ
                     LCFVLPEFACEQSTGGGCEQGSSKPSDKAAGTEQGNSGAGDKPKKPSRHRRRY"
     gene            complement(1363..2529)
                     /locus_tag="PK710_08590"
     CDS             complement(1363..2529)
                     /locus_tag="PK710_08590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT09814.1"
                     /translation="MTETFDAIVVGGGSVGVPSAYSLAKRGLKVLVLERRSAVGQGEN
                     KAAIGGVRATHSDPAKILLCTRSLEAVSTWKQVHGDDIGWKPGGYCFPAYSEAVESTL
                     QGLLPKQRAFGLVNDYVDADAVAELIPGIVREGLRGGTYSPNDGQVSPLLLPVILMRH
                     AERMGAQLRVHEQVTEYLVEAGAIRGVVTNQGRYEAPIVVLATGSDAARDGQLLGIPI
                     PVTPDSHEAGITAPVEPFLAPLVVDLRPGPENKTVNFYFGQNHEGQIIFCYTPKDLFV
                     GTNRESLSEFLPTLAARMISLVPRLKHLLVRRVWRGCYPMTPDGVPIVSNVDGIRGLT
                     LAVGMCGQGFMLGIGVGEEVASLALEGKYLLPKEASDTLRFERDYSSGKKEALK"
     gene            complement(2522..4630)
                     /locus_tag="PK710_08595"
     CDS             complement(2522..4630)
                     /locus_tag="PK710_08595"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT09815.1"
                     /translation="MSAKEPIFAPESLRIVDHPILRPAAPATVPFTFNGQPLLARPGE
                     AISSALYAAGISIFGHHHRDGGAQGIFCVNGQCSQCTVLVDGRPLKSCMTLVREGMVV
                     QSCEGRPSLPHDDHVEALGRPGEIDVPVLILGGGPAGLCAAIELGRLGVEVLIIDDKP
                     SLGGKLTLQTHQFFGSVEDCWAGTRGVDIGHILEKQVQELPTVRVWLDSTAVGVFCDG
                     KVGVVTPQGYRLVHANSLLVALGAREKTLSFPGCDLPGVVGAGAFQTLVNRDLIRTCS
                     KLFVLGGGNVGLIGAYHALQAGIDVVGLVEALPEVGGYKVHADKLRRLGVPIWTRTTV
                     VRADPAPGKETVGSVTIASIDDRFQPIAGTTRTFEVDTLLIAVGLSPVDELLEKARQL
                     GIKAYAAGDTAEIAEASAAIFSGRITGRQMARDLGIPVEIPSYWPELAAILRSRPGKT
                     TPFEPRELDQPVHPILRCVQEIPCNPCQQACPDKLITMPGSIMALPTFSGKCLGCGEC
                     VTVCPGLAINLVFNDYDPTGQKALLMLPYEFSLDRLPLGSQVDTVDIDGNPVGKGTVM
                     AVRSRETQDRRHLLLVEVPAQDKLKVAGFTIRKPSVPIDGLPMSGDDDPIVCRCERVR
                     KSAIVAEIRAGVRDINQLKALARVSMGGCGGKTCTDLVRRIFLEEGVEWSTVTSGTVR
                     PLVAETPLGAFVRGEESPHD"
     gene            complement(4627..6570)
                     /locus_tag="PK710_08600"
     CDS             complement(4627..6570)
                     /locus_tag="PK710_08600"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT09816.1"
                     /translation="MNSATQPLVSLRIDGRPVQAPPGAFVLDVARGMGIDIPTLCHHP
                     DLESVGACRLCVVEVTHPEWKGWSGLMTACLYPVKDGIEVYTRSDKVLAARRGILSLL
                     LARCPNSDIIRSLAEQAGARIDGLTTDPNADNCILCGLCTRICETYATGAITTVSRGA
                     VKRVSSFANQPPEECIGCGACVIVCPTGEIQGQRTPTDYQVWERSFPTAICTVNTAAC
                     IGCGSCEEACPFSVARVALRAGGDQMAVIPEEHCRGCGVCVGACPTGAITQRGLESNV
                     VGQPKPTIPSVFACARSNLTSSESAHLFEMPCVGRISTPLLLHAMASGAPGVLVLGRH
                     QSTCRLNGSEDSAQARVRAARDALDLLGLDPRRIQFEEPAPGPDGPRASVARFQSMLA
                     SLEKPESLSLPPLEHEGLDTSLALINKMSRSAVETDGGARFLEKHGLPTPSLHGPVLL
                     AGCIPILHTLGKELFAPLSLVDALRQALDVLEHLGIIGTGIRIGAPKSAFALEKCGDV
                     TLLDDVLRGRGRDLPRPAQGMKVAFDGTEANRILIEALGYEPVDIGSDPLPDHFSLTA
                     QDRKRAVGTLAEAERAGAHALLVANPRALARWAMMTRAGSWRSHRVQPILGVQLAYHA
                     VHGLSLAPTHPNRVSSPCMQVAP"
     gene            complement(6567..8945)
                     /locus_tag="PK710_08605"
     CDS             complement(6567..8945)
                     /locus_tag="PK710_08605"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOT09817.1"
                     /translation="MQLDLAKVDAILERYPADESSLVMVLQDVQSHFHFLPSEALEHV
                     ADRLDLPRSKVFSVATFYKVFSLSPKGRTVIQVCKGTACHVRGAQLLEDELSRQLRIR
                     AGETTEDGAFTLETVNCVGACAMAPVVVTGQSIHAEVVPAKIRTLLKKASISSEKKRT
                     KQTSDSFSKRMSSPAALLARSEELRSMWEAIPTKVVVCGGPGCLAAGALEVMEALQTA
                     ASEQRLPIETELGACSHDHSLLSLSGCQGLCQQGPLVQVYPDDIQYIKVKPKDASSII
                     SAIIERRVESSLVGESQGRAQHPFYQGQVFRSLSLCGQVDPESLDHYLALGGFVSLAQ
                     TLANRTPEQVIELVDASGLRGRGGAGFPTGKKWRSAIAAAKAEGRPPYVLCNGDEGDP
                     GAFMDRAILEGSPYQVIEGMILGAYALRAREGYLYVRAEYPLAVERLQKAISTCESAG
                     LLGERILGSSLSFRLQISRGGGAFVCGESTALMRSIEGKVGEPRAKYTRSVERGLYDA
                     PTVLNNVETWALIPGIVRDGAEAFAAIGTKRSKGTKAFSLTGQVRRTGLVEVPMGTTL
                     RQLIFDIGGGVLPGRTFKAVQTGGPSGGCLPESQLDLPVDFDALTEAGSMMGSGGMIV
                     MDDTSCMVDVALYFVRFLLEESCGKCAPCRLGLPQLAFLLTKLTEGKGTLQDIETIEA
                     IAATMEHNSLCGLGKSAPNPVLSTLRYFRHEYEQHVRGRCPAGVCRELIRYEVMDTCT
                     GCLACIKPCPENAITGEFEKIHIIDQALCSRCGICRSVCNFDAIRVVSGGEP"
     gene            9137..10624
                     /locus_tag="PK710_08610"
     CDS             9137..10624
                     /locus_tag="PK710_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HOT09818.1"
                     /translation="MAKLLETRSPHGVYGRPVLFNTLQFAWFFLAVFVISWLLVGRPT
                     LRRLFLLAASYYFYGSWDWRFLGLLVLSTVIDFVVGYRMSIAPKHKRRGLLLLSVVSN
                     LGILGFFKYYNFFIDSVVTTLHSVGLHADIPTLQIILPVGISFYTFQTMAYTIDVYRD
                     RSEVCKDPLTFALYVSFFPQLVAGPIERAQNLIPQLARDPTAFSQSEAGKALFLIAIG
                     LIKKVVIGDYLAINLVDRVFTSPSSYSALEVLAGVYGYATQIYCDFSGYTDIAIGCGI
                     LMGVRFNLNFNSPYKAHNLQEFWHRWHISLSTWLRDYLYIPLGGSRNGSFNTYKNLMI
                     TMLLGGLWHGAGWQFMFWGFLHGGLLGVTRAWQRWRGTEGAYVFPTTAWGLLKHAFFV
                     LLTFHYVCLGWVFFRAESFGKAWAVLKQISTLTTYHPNLHGWVLLALVVGLVSHYTPD
                     SLFEWARSKFAALPWYVQGLLLFVVAFVLREAASADAVPFIYFQF"
     gene            10742..11506
                     /locus_tag="PK710_08615"
     CDS             10742..11506
                     /locus_tag="PK710_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOT09819.1"
                     /translation="MMFGFRIIRAPNKGLVFTLGKPSRVLDPGIHWVSPMLCQVVLVS
                     TALHTFEVDIDVITKGGTPTTIKVGYTARIVDPYAAVVNVADPFMTLRASVISVASGA
                     ANSYTIDQLAQYKSQIAHMAQQELAGLSQANGWGLGDFQIAIGDPSMSNELKGLLMRE
                     EAVRRENAANLERARNQLAIAQQLAAVAAQLEHSPFARELLRLQMIADMGAGGKVIVV
                     DSQSPSAKAVAMQASGQQPLAPQATHHTQPVYPQTT"
BASE COUNT         2522 a         3491 c         3207 g         2411 t
ORIGIN      
        1 aaaaaacttg gccaactatc ttgcgttttt tcttcccaaa gtacgccccc atggtaagcg
       61 gacacagggg gtgttcagaa tgccgtcttg gagaatggga agatgaccaa aaagcacacc
      121 gtccaagagg tatccgctcc gccaatcgtg tccttcccgc ctcgagattc cacacctacg
      181 atgcccccat ggcaaggcca gccagcccac cagctcccca atccccaatc ccctatccac
      241 gaagcgattc tgccgctgcc ctgccaccca ttcgattcgc ccctcgccac caaacccact
      301 catcaacgac gaaacgcaag acccgagttc gtcacgagcg cgaaatcaat atcgtcgtct
      361 gtgacggctt ggctttttgg gcttgtcacc ggcgccgcta ttgccttgtt cagtacccgc
      421 ggccttgtcg gatggcttgg aactaccctg ttcgcagcca ccaccggtag actgctcaca
      481 agcaaactcg ggcaagacga agcataattg gtcggcagaa acgacctcac tacgatgctg
      541 acgaaaccct gattgcatcc gttgcacagc ttcctgaagg gtagcgttat gcgcggaaag
      601 ggaagcaagc ttttcctcga gttgcttgat gcgctgcatc aaggcatcca cgttttgcga
      661 ctgtgattcc gagcgttgct gtatatactg gaatattgct tgggctgcct ccgccgatga
      721 aaaacactct gcgcttgtct cgtaggtgca tggacaatag agattgttta tccttgtaga
      781 ggccatgatg tcgagagtct ataaacactt tataagctat tagcaagaga tatttaaggg
      841 ggagatgaat tatttttgtc cctctggcga cgaggtcagt gataggttgg gggatggcga
      901 tagggctggg gagagcagcg agcaaccgtt ccagatcctg agccatcccc tcattatttc
      961 cgagtctttc cttcacgatc acggccacgg cgcacgcaaa ccggtcacgg tcacggtcac
     1021 ggcgcacggc ccacgcaaac cggcaatggc gataggaatg gggagagcag cgagcaaccg
     1081 ttccggatcg gcctgggaga tttggatatg cgcatcaaca agtagggcaa attcgaaacg
     1141 tagtcttatg cgtctgaaaa acgcgagcaa caaaaccgga tacgctataa cgtaaaacca
     1201 ctctctcccc ccttccaccc acactgtggg gggaagggcc ggggatgggg gcccacactg
     1261 tggggggaag ggatggggat gggggaaaca aacaacggct ggggatgagg gggaacaaac
     1321 aacggcaggg gatgagggag gacaaaaagc caggggcggg gcctacttca acgcttcttt
     1381 cttgcccgat gaatagtcac gctcgaatcg caacgtatcc gacgcttcct tgggcagcag
     1441 atacttaccc tcgagggcaa gcgaagcaac ttcttccccc actccgatcc cgagcatgaa
     1501 tccttgtccg cacatgccca ccgccaacgt caacccacga atgccgtcca cattgctgac
     1561 gatcggcaca ccgtcgggag tcatgggata acatcctcgc cacacgcggc gaacgagaag
     1621 gtgtttgagc cgcggcacga ggctgatcat tctcgccgca agagtgggaa ggaattccga
     1681 aaggctttct cggttcgttc cgacgaacaa gtccttgggc gtataacaaa aaatgatctg
     1741 cccctcgtgg ttttggccga agtagaaatt gacggttttg ttctccggtc caggccgtag
     1801 atccaccacg agcggtgcga gaaagggctc gacgggtgcc gtgattcccg cttcatggct
     1861 atcgggcgtg accgggatgg gaatccccag caattggcca tcgcgcgcgg catccgagcc
     1921 agtggcaaga acgacgatcg gcgcttcgta gcgtccctgg ttcgtgacca cgcctcggat
     1981 cgctcccgcc tcgaccaggt attccgtcac ttgttcgtga acacgaagct gcgctcccat
     2041 tcgctcggca tgtcgcatca ggatgacagg cagcagcaag ggcgatactt gaccatcatt
     2101 cggtgaatac gtgcctcccc gaaggccttc tcggacgata ccgggaatga gttccgctac
     2161 cgcgtcggca tcgacataat cgttgaccaa tccgaaagcg cgttgtttgg gcaacagtcc
     2221 ctgcaaggtg gattcgacgg cttcggaata ggcgggaaag caataaccgc ccggtttcca
     2281 accgatatcg tcgccatgga cttgtttcca cgtgctgacg gcttcgagac tgcgcgtgca
     2341 aagcaggatt ttggccggat ccgagtgggt agcgcgcacg ccaccgatcg cggccttgtt
     2401 ctctccctgg cctacggcgg aacgacgctc caacacaagg accttcaatc cgcgctttgc
     2461 gagggaatag gcggaaggaa cgccgacgga cccgccgcct accacgatgg catcgaaggt
     2521 ttcagtcatg ggggctctcc tcaccacgaa caaaggcacc aagcggtgtt tccgcgacca
     2581 aagggcgcac cgtgccggat gtgaccgtcg accactccac accttcttcc agaaagatgc
     2641 gtcgaacaag gtcggtacac gtcttgccac cgcatccacc catgctgacg cgggcaagcg
     2701 ctttgagctg attgatatcg cgcactcccg cccgaatttc cgcaacaatg gcgctttttc
     2761 gcactcgttc gcagcggcaa acgatcggat cgtcatcgcc ggacataggc aggccgtcga
     2821 tgggcacgga cggttttcgt atcgtaaatc cagcgacttt cagtttgtct tgggccggga
     2881 cctcgacgag caaaagatgc ctgcggtctt gcgtttcccg gcttcgaacc gccatcaccg
     2941 ttccttttcc cacggggttg ccgtcgatgt ccaccgtgtc gacctggctt ccgagaggca
     3001 atcggtcgag ggagaattca taaggcagca tgagcaacgc cttttgaccg gtgggatcgt
     3061 aatcgttgaa caccagattg atggcgagac cagggcaaac cgtcacgcat tcaccacatc
     3121 cgagacattt ccccgagaat gtgggcaacg ccatgatgga accgggcatg gtaatcagct
     3181 tgtcggggca agcttgttga cacggattgc acgggatttc ctgaacacat cgaaggatgg
     3241 ggtggacggg ttgatcgagt tcccgaggct cgaaaggcgt ggtttttcct ggccgactcc
     3301 tcaatatggc cgcaagctcc ggccagtacg acggaatctc cacgggaatc ccaaggtccc
     3361 gggccatctg tcggcctgtg atccgtccgc tgaagatggc agccgaagct tcggcaattt
     3421 ccgccgtgtc tccagccgca tacgccttga tcccaagctg tcgcgctttt tccaagagtt
     3481 cgtccaccgg gctgagcccc accgcgatga gaagcgtatc cacctcgaat gtgcgtgtcg
     3541 ttcccgcaat cggctgaaat cgatcgtcga tgctggcgat ggtcacactg cctacggttt
     3601 ctttgccagg ggctggatcg gcgcgcacga cggtcgttcg tgtccagatc gggacaccca
     3661 gacgccgaag cttgtccgca tgaaccttgt atccgccgac ttcgggcaga gcttcgacca
     3721 atccgaccac atcgataccg gcttgcaagg catggtaagc gccgatcaag ccaacgttgc
     3781 cgccgcccaa gacaaatagc ttggaacacg ttcgaatgag atctcgattg acgagggttt
     3841 gaaacgcgcc cgcgcctacc accccaggca aatcacaacc tgggaacgat agcgtttttt
     3901 ctcgtgcccc aagggctacc agcaaggagt tggcatggac cagccgatac ccttgcggtg
     3961 tgacgacacc gaccttgcca tcgcaaaaaa cgccgaccgc ggtgctatcg agccatacac
     4021 gaaccgtcgg caattcctgc acttgctttt cgagaatatg accgatgtcg acaccgcgcg
     4081 tgcccgccca gcaatcttcc acggacccga aaaattgatg cgtttgcagc gtgagcttcc
     4141 cccccaacga aggcttgtcg tcgatgatca acacttcgac tcccaagcga cccaactcga
     4201 tagccgcgca caatccagca ggacccccgc cgagaatgag aaccggaacg tcgatttctc
     4261 caggacgtcc caaggcttcc acgtgatcgt cgtggggcaa cgacggacga ccttcgcagc
     4321 tttgcacgac catgccttca cgaacgagcg tcatgcagga cttgagcggg cgcccatcga
     4381 ccaacacggt gcattgggag cattggccgt tgacgcagaa aatgccctgg gcgccaccat
     4441 cccgatgatg gtgtccgaag atcgaaatgc ccgcggcgta cagggccgaa ctgatcgctt
     4501 ccccaggccg ggcgagcagc ggttggccgt tgaaagtgaa gggaacggtt gcgggggcag
     4561 ccggacgcag gatcggatgg tctacgatcc gcaaggattc gggggcgaat atgggttctt
     4621 tggcgctcat ggtgccacct gcatgcaagg agaggaaact cggttgggat gggtgggcgc
     4681 gagagaaaga ccgtgaacgg cgtgataagc gagctgcacc cccaaaatgg gttgcacacg
     4741 gtgggaacgc cacgaccccg cccgcgtcat catcgcccaa cgcgccagcg cccgcggatt
     4801 ggcaaccaac aaggcgtgcg cccccgctcg ctccgcttcg gccaaagtgc ccaccgcgcg
     4861 cttcctgtcc tgcgccgtca gtgaaaaatg gtccggaagc gggtccgatc caatatcgac
     4921 cggttcatat ccaagcgctt caataagaat ccgattcgcc tcggtcccgt cgaacgccac
     4981 cttcattcct tgggcgggac gaggcaaatc ccgccctcgt ccgcgcaata cgtcgtcgag
     5041 caaggtcaca tcaccgcatt tttcgagagc gaaagcggac ttgggtgcgc cgatacggat
     5101 gcccgtaccg atgatgccaa gatgctcgag gacatcgagg gcctggcgca acgcatccac
     5161 caaggaaagg ggagcgaaaa gctccttccc gagcgtgtgc aggatgggaa tgcagccggc
     5221 gagcaatacc ggaccatgca gcgatggagt cggaagacca tgtttttcta gaaaccgagc
     5281 gcctccgtct gtttctaccg cgctccggct catcttgttg atgagcgcaa ggcttgtgtc
     5341 gagcccctcg tgttcgagag ggggcaacga caaagattca ggtttttcga gggaagccag
     5401 catcgattgg aagcgcgcga cagaagcgcg tggaccatca ggccctggcg cgggctcttc
     5461 gaactgtatt cggcgagggt cgagacccaa gagatcgaga gcatcgcgag cggcgcgaac
     5521 ccgtgcttgg gcggagtcct ccgaaccatt gagccgacag gtcgattgat ggcggccgag
     5581 aacgagaacg cctggcgcgc cggaggccat ggcgtgcaag agcagtggcg tggaaatcct
     5641 tcccacacag ggcatttcga aaagatgggc ggattcggaa gacgtgaggt tcgaacgcgc
     5701 acaggcaaac acacttggta tggttggctt cggttggccc acgacattgg attccaggcc
     5761 gcgctgggta atcgctccgg tggggcaagc gccgacacaa actccgcaac cccggcagtg
     5821 ttcttcaggg atgacggcca tttggtcacc gcccgctcga agcgccacac gcgcgacaga
     5881 aaacggacat gcctcttcgc aggaaccaca gccgatgcac gcggcggtat tcaccgtgca
     5941 gatggccgtc ggaaacgagc gctcccacac ctggtagtcc gtaggcgttc gttgtccttg
     6001 gatttcgcca gtggggcaaa cgatgacaca ggcgccgcat ccgatgcact cttcgggagg
     6061 ttggttggcg aaactcgaaa ctcgtttgac cgccccacga ctcaccgtgg tgatcgctcc
     6121 cgtcgcataa gtctcgcaaa tacgcgtaca tagaccgcat agaatgcaat tgtccgcgtt
     6181 gggatcggtg gtcagcccgt cgattcgcgc tcccgcctgt tccgccaggg aacgtattat
     6241 atccgaattt ggacaacgag caagcagaag ggataggatt ccccttctgg cagcgagtac
     6301 tttgtcgctt cttgtatata cttcaatacc atcctttacc ggatataagc aggcggtcat
     6361 caaaccgctc cagcctttcc attccgggtg ggtgacttcg acgacgcata gacggcatgc
     6421 gcccacggat tcgagatccg gatggtggca aagcgtgggg atatcgattc ccatgccgcg
     6481 cgcgacgtcc aacacgaacg cgcctggggg cgcttggacg ggacggccat cgatgcgcag
     6541 actgacgagc ggttgcgtgg cggagttcac ggttcacctc cggaaacgac tcgaatggcg
     6601 tcgaagttgc aaaccgaacg acagatgccg cagcgagaac acaacgcctg gtcgatgatg
     6661 tggatctttt cgaactcgcc tgtgatggcg ttttcagggc atggcttgat gcatgcgagg
     6721 cagcccgtgc atgtatccat gacttcgtag cgaatcaatt cgcggcatac ccccgcaggg
     6781 caccgccctc gaacgtgctg ttcgtactcg tgccggaaat aacgaagcgt ggacagcacg
     6841 gggttgggag cgctcttccc aaggccacac aaggaattgt gttccatggt ggcggcgatc
     6901 gcctcgatgg tttcgatgtc ctgcagcgtc ccttttcctt ccgtgagctt ggtcagcaaa
     6961 aacgccagtt gcgggagccc aagacgacag ggagcgcact ttccacagga ttcctcgagc
     7021 aaaaatcgaa cgaagtacag cgccacatcc accatgcacg aggtgtcgtc catcacgatc
     7081 atgccgccgg accccatcat ggatccggcc tccgtcaacg catcgaagtc gacaggcaag
     7141 tcgagctggc tttccggcaa gcatccgccg gaagggcctc ctgtctggac cgctttgaac
     7201 gttcttccag ggagaacacc cccgccaatg tcgaaaatga gttggcgcag ggtcgtgccc
     7261 atgggcactt ccaccaaccc cgtgcgacgc acttgccccg tgagggagaa tgctttcgtc
     7321 cctttcgatc gtttggtgcc aatggcagca aatgcctcgg cgccgtcgcg aacgatgcca
     7381 gggatgaggg cccaggtctc gacgttgttc aataccgtgg gtgcgtcgta caagcctcgt
     7441 tccaccgatc gggtgtattt ggcgcggggt tcacccactt tgccttcgat gcttcgcatc
     7501 agcgcggtgg attcaccaca gacgaaagcg ccgccgcctc tggagatctg caaacgaaat
     7561 gacagggaag atccgaggat gcgctcgccg agcagacccg cgctttcaca cgttgaaatc
     7621 gctttttgca accgttcgac cgcaagagga tattcggcgc gaacgtagag atacccttcg
     7681 cgggcccgaa gggcataagc tcccaagatc attccttcga tgacttgata agggctccct
     7741 tccaagatcg cccgatccat gaaagctccc ggatcccctt cgtcgccgtt gcaaagcacg
     7801 tatggtgggc gtccttcggc cttggccgcg gcaatcgcgg atcgccattt ttttcccgtg
     7861 ggaaaaccag ctcctccacg accgcgaagc cctgaagcgt cgacgagttc gataacctgt
     7921 tccggggtcc tgttggccaa cgtttgtgcc agcgaaacga agccaccaag cgccagataa
     7981 tgatcgagac tctcgggatc tacttgtcca cataaggata gcgatcgaaa tacctggccc
     8041 tggtaaaaag gatgctgggc gcgtccttgg gattccccca ccagcgacga ttccacgcga
     8101 cgttctatta ttgctgaaat tatggaagaa gcatccttgg gcttcacctt gatgtactga
     8161 atgtcatcgg gataaacctg caccagaggc ccttgttggc atagtccttg gcatccggaa
     8221 agggatagaa gcgagtggtc gtgggagcaa gcgcccaatt cggtctcgat gggcaatcgc
     8281 tgttcggacg cggcggtctg aagagcctcc atcacctcga gggcgcccgc cgcaagacat
     8341 ccaggaccac cgcataccac gactttcgtc ggaatcgcct cccacatcga tcgaagttcc
     8401 tcgctccttg ccaaaagagc agcgggggaa ctcatgcgtt tcgaaaatga atcggatgtt
     8461 tgcttcgttc gtttcttttc ggaggaaata gaagcctttt tcaataaggt tcgtatcttc
     8521 gccggaacca cctccgcatg gatgctctgt cctgtcacaa cgaccggcgc catcgcgcaa
     8581 gcacccacac aattgactgt ttctagcgta aacgcgccat cttccgtcgt ctcgcccgca
     8641 cggatgcgaa gctgccgact cagctcatcc tcgagaagtt gcgcccctcg cacgtggcat
     8701 gccgttcctt tgcatacctg gatgaccgtt cgtcccttgg gcgacaaact aaataccttg
     8761 taaaacgtcg ctaccgaaaa caccttcgaa cgcggaaggt cgagcctgtc cgccacgtgc
     8821 tccaacgctt cggaaggtaa gaagtgaaag tgagactgca catcctgcaa caccatcacc
     8881 agtgatgatt catcagcggg atagcgttcc aggatcgcgt cgaccttggc gagatccagt
     8941 tgcatgcatg ggctcctgcg gtggtggccg agtgccgtcc ttctatctgg tggaccaggc
     9001 tgtctcacac ggggattagg gggactgccg gctacgcctc catagcacac agcgcctccg
     9061 cggggatggt gaaacccacc cctcccttga cacgaaccca acagtccccc gtcgaaagag
     9121 caatctctcc caagaattgg caaagctgct tgagacgcgc tcgcctcacg gggtatacgg
     9181 ccgcccagtg cttttcaaca cgcttcaatt tgcgtggttc ttcctcgccg tcttcgtcat
     9241 tagctggttg ctcgtcggaa gacccaccct tcgacgcctc tttctgcttg ccgcaagcta
     9301 ctatttctac ggctcctggg attggcgctt ccttggattg ctcgtgctgt ccaccgtgat
     9361 cgacttcgtg gtcggttatc ggatgagcat tgcccccaaa cacaagcgac gcggccttct
     9421 cttgctcagt gtcgtctcca acctcggcat cttggggttt ttcaaatact ataatttttt
     9481 catcgactcg gtcgtgacca ccctgcattc cgtcggactt cacgccgata tccccaccct
     9541 gcagatcatc ctgcccgtgg gcatcagctt ctatacgttt caaaccatgg cgtacaccat
     9601 cgatgtgtac cgagaccgct ccgaagtctg taaagacccg ctgacgttcg ctttgtatgt
     9661 ctcgttcttt ccccaactgg tggcaggccc gatcgaacgt gcgcaaaatc tcatccccca
     9721 gcttgctcgc gatcccaccg cctttagcca atcggaagca ggaaaagctc ttttcctcat
     9781 cgccatcggc ttgatcaaaa aagtcgtcat cggcgattac ctcgccatca atctcgtcga
     9841 tcgtgtgttc acttcaccga gcagctattc cgcgctcgag gtgcttgccg gtgtgtacgg
     9901 ctacgccaca caaatctact gtgacttttc cggctatacc gatatcgcca tcggttgcgg
     9961 catcttgatg ggggtgaggt tcaacctgaa cttcaattcc ccctacaaag cgcataatct
    10021 tcaggagttc tggcatcgct ggcatatctc cttgtcaacc tggcttcgcg attacctcta
    10081 catccctctg ggtggttctc ggaacggctc gttcaacacg tataaaaatc tcatgatcac
    10141 catgctgctg ggcggtcttt ggcacggcgc ggggtggcag ttcatgttct ggggcttctt
    10201 gcacggcggc ttgctcggcg tgaccagagc ctggcaacga tggcgtggaa cagaaggtgc
    10261 ctacgtattt cccaccacgg cttggggact gctcaaacat gcctttttcg tcctgttgac
    10321 cttccattat gtctgcttgg gatgggtttt cttccgcgcg gagtcgttcg gaaaagcatg
    10381 ggccgtgctg aagcaaatct cgacactcac cacctaccac ccaaacttgc atggctgggt
    10441 cttgctcgct ctcgtcgtgg gattggtatc ccactacacc ccagactccc tgttcgaatg
    10501 ggctcgctcc aagttcgccg cgctcccctg gtacgtccaa ggactgctgc ttttcgtcgt
    10561 cgctttcgtc cttcgtgagg cggcatccgc cgatgccgtt ccgttcattt atttccagtt
    10621 ctaaattcgc ttcgcaacca cacttgatgc ccatagtcgt gacgctatgg atcgtatctc
    10681 gacttgacgt ggacgtcgga agcctgcacg aagtcacaac cagtcgttgg tatggagaga
    10741 aatgatgttt ggctttcgga tcatccgtgc tcctaacaag gggctcgttt tcacgctcgg
    10801 aaaaccatct cgagtcctgg atccgggcat ccattgggtg tctcccatgc tctgccaggt
    10861 cgttttggtg tcaaccgcgt tgcatacctt cgaagtcgac atcgatgtca ttaccaaagg
    10921 cggaacgccc acgaccatca aagtcggtta tacagccagg atcgtcgacc cttacgccgc
    10981 cgtcgtcaat gtcgccgatc cgttcatgac cttgcgggca agcgtgatct cggtagcctc
    11041 gggagcggcc aattcctata ccatcgatca gctcgcgcag tacaaatcgc aaattgccca
    11101 catggcccaa caagaacttg cgggcctgtc tcaggcgaat ggttggggac ttggtgactt
    11161 ccaaatcgcc atcggcgatc cgtccatgag caatgaactc aaaggattgc tcatgcgaga
    11221 agaagccgtt cgtagagaaa acgcggcgaa tcttgagcga gcgcgcaatc aactcgccat
    11281 tgcgcagcaa ctcgccgccg tggcagctca gctcgaacat tctcccttcg ctcgtgaact
    11341 gcttcgtttg cagatgattg cggatatggg cgctggcgga aaagtcatcg tcgtcgattc
    11401 ccagtctccg tccgcaaaag ccgtcgccat gcaggcaagc ggacagcagc ctctcgctcc
    11461 gcaagcaacg caccatacac agccggttta cccacaaacg acatagggcg acgcgtcgta
    11521 gacagttcca cgggcgctca cggtcacggc ctacgcaaaa cgctcacggt catgctaacg
    11581 acccaagcaa aacgacccaa gcaaaacgac ccaagcaaaa cgacccaagc a
//
LOCUS       DAOSOR010000119        11615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5527_length_11615_cov_4.802336, whole genome shotgun sequence.
ACCESSION   DAOSOR010000119 DAOSOR010000000
VERSION     DAOSOR010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11615
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5527_length_11615_cov_4.802336"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..312)
                     /locus_tag="PK710_08620"
     tRNA            complement(240..312)
                     /locus_tag="PK710_08620"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(277..279),aa:Ala,seq:cgc)
     gene            618..2543
                     /gene="dnaK"
                     /locus_tag="PK710_08625"
     CDS             618..2543
                     /gene="dnaK"
                     /locus_tag="PK710_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOT09820.1"
                     /translation="MGKIIGIDLGTTNSVVAVMEGKDAKVIVNEEGSRLTPSVVAWDD
                     KGEILVGQIAKRQSVTNPENTVYSSKRFVGRRYVEVEQERSRVPFKVTQGPNGDAWFE
                     VRGKKLAPPEVSAKVLQKLKKAAEDYLGQPVTEAVITVPAYFNDSQRQATKDAGKIAG
                     LEVKRIINEPTAAALAYGLDRKADEIIAVYDFGGGTFDISILEVGDNVVQVISTNGDT
                     HLGGDDLDNLVMDWLINEFKKETGIDVSKDKMVIQRLKDAAETAKIELSSKMETSINL
                     PFLTADASGPKHLQTTLSRSKFEQMIRPLVDRTLTPVRKALEDAKKRAEDIDEVVLVG
                     GSTRVPLVYDSVKQFFGKEPHKGVNADEVVSIGAAVQAGVLSGDVKDMVLLDVTPLSL
                     GVETLGAVMTVMIPRNTTIPTQKKEIFSTAADNQPSVEIHVLQGERTEARYNRTLGRF
                     HLEGIMPAPRGVPKIEVTFDIDANGILSVAAKDLATGKDQRITITANSGLSDDQIQNM
                     VSEAAANEADDKRRRDEIERRNRLDNMCYQLEKTLNENKDKLPSSDVATLQGIIMEGK
                     QAVEKQDDALVQEALEKLEKEAHRIASVMYQQAGPQAGAEGPGAPAGAASDGSSDDSN
                     GSKKRQDVIDAEFEESN"
     gene            2699..4003
                     /locus_tag="PK710_08630"
     CDS             2699..4003
                     /locus_tag="PK710_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOT09821.1"
                     /translation="MSIFTRAMVGGAMVLAAVVGCGSDDTQPGPSASSGGSAGQGGTG
                     AAGVGGEGGSGATSVGGMGGTGGNAGQGGEAAGSVGGAAGETAGSGGVAGSGGTVPSG
                     GSGGGAALTRMTISGDATWTVTFDAQAKAKGATDCQYTRHYEGVQDQSRPWVCEQCDV
                     IFRTTVTMPVGLNDCYKQVSDVDPMDEEWVGYGNGTWYRSYGIGASGQGDVEITGQSL
                     TYTNEVKDLEADIVGAGKMDFLVEGAFTTSQEDGDPMHGWVAPAQYACGWDKADPAEY
                     SGPYSLKVGSTLPDGVFRDACDEPYRLHDAKGAYLVVFMSAMDCPACRTLASGESSFL
                     ADLKAEGIEARTITLMAPSLNDPFGDTTKAQLTYWGDKYSIPTPIVADRGWGITMFLP
                     IYNDKLGYPSWVVADPSLKVLNYGTGGTWATIKNTILKDAKP"
     gene            complement(3969..5810)
                     /locus_tag="PK710_08635"
     CDS             complement(3969..5810)
                     /locus_tag="PK710_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT09822.1"
                     /translation="MSANSSSRTERVLRDFHEESEVVGAFDRRLAARLWPFIRPQSKG
                     LALTMGMLALISALALLRPLIMRSAFEGATTQTLTRAGFLLLVVIVGEQILTFAQMYS
                     MQVVGARAMHDLRRGVFEFLHTQRLQFFDRRPIGRLVTRVTNDTDSVGELFASGALNA
                     FGDVLRLFGIVILMLALDWRMSLIAFALVPLIGIFVEIIRRKARTAYRSIRTKLARLN
                     AFLNEQVQGIAVVQSFCREKENAIEFDDINLAYRNAHFRAISLEASLDATIEMVSSIC
                     IASILWYAGVKTFHADVSFGTLVAFVAYIEQFFVPIRDLSSRYTIFQSAMAGAERVFE
                     LLDHPEPDAVVSTHGSHATPTFAFEFDHVSFGYHPNTPVLHDICLQARHGEKIAIVGP
                     TGSGKSTIASLLLRLYDPQQGVIRIEGRDVHCIDRQELRQHFSVVPQELYLFSGTLAS
                     NVAVGQHIPDRKRVEEGLRRVGAGDLIDDRPGGIDTRIDERGTNLSVGQRQLVAFARA
                     LYRDAPIVLLDEATASVDSTTEAKLQGALAELMKGRTALIIAHRLSTIRSADRIIVLH
                     HGRIVEQGSHDELMHRRGLYAKLHALQLSRESAPTALTASHPSESYS"
     gene            complement(5807..7576)
                     /locus_tag="PK710_08640"
     CDS             complement(5807..7576)
                     /locus_tag="PK710_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT09823.1"
                     /translation="MSHHHSLGAQLRKQLPSYLAGTVLLAILQTLMSLRDRLFKTGVD
                     AAIAANESLTIRTVVFILVLVAIAAAVRVLSRITIFHAGRNAEYQIRADLIDHLHRLG
                     FSFFQKIPTGEIMSRATNDLGQVRLLLGFGVLNVINTLFALVSALSVMIEISPRLTLA
                     ALAPFPLLILVTRLYGRVVFSRTTANQQALGQLANRVQESLVGVRIVRAFSLEKAELR
                     DFDTASQHYLDKALALARARGMLVPMMGVIGAVGMIVVFWYGGWLVLQHTITEGDFIA
                     FWSALARLTWPIMAFGFVISIVQRGRAGYARIKEILDTIPDITDGPLNAPTSIHGDLR
                     VENLHFSFGDSNVLTGVSFHVPAAHSLAIVGKVGSGKSTLAALLARLLPTPKGTVFLD
                     EHDVCDLPLSTVRRAIGYAQQDAFLYSTTVGRNIAFTLDDPESPDGQERIRFAASEAQ
                     ILEEIQLLPDGMDSVVGERGLQLSGGQKQRVALARALLYEPTILLLDDPLSAVDTQTE
                     QAILKAIDRQAQRRTVLLITSRVTAAARCNHIVVLDQGRIAEQGTHEQLIRKPGIYAR
                     FAEEQRLQGEIDSIDAHGLGDTP"
     gene            complement(7621..8958)
                     /locus_tag="PK710_08645"
     CDS             complement(7621..8958)
                     /locus_tag="PK710_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitochondrial 54S ribosomal protein L40"
                     /protein_id="HOT09824.1"
                     /translation="MSPLFLELNEGDFDAYLPERASSNAFSRPRLEFKQRALGWAKSV
                     ADRLHSIGIPVDMEASDEHPSVRNNHRVDEQWIIFWRPAEQRQYLDPLLDQHAGIAQS
                     LRNASPWFRHAFLALRLDSEHVEVCACLHPDAWIDLQAFHAKLNDDDRAQSIIDTILA
                     LPEEFQFGVSGQSPLPIASASIPALRELAQSLDEHRAALWFGWWISRATALQHAQLLG
                     EQLEDALIALGPLYQILAWQQQDDVSELGRSLEAMHIQRSQAAAQRAEAERMQQQESE
                     RLRQVRIEQSREITRERVEYDSNRSRPTLATLFKSTSSTAATPPSPATPRTATPTPPA
                     AIPHNRPVPRAREFTPKPKTADRPPPPQPQPAFTAGGTLDKGAQVRVLSGPFVGKIGI
                     VAELDTRGNARVMLGLLSTRLLAEQLEAVVDSNERPSLQSSHRKNSLLTRSGK"
     gene            9183..9557
                     /locus_tag="PK710_08650"
     CDS             9183..9557
                     /locus_tag="PK710_08650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09825.1"
                     /translation="MTFVVVLHEERMDAMPTRCSMCRTRRVQSQAHQQTPKYTGDRNE
                     YRSPMACEYPEAPQRGSSFRAGGRKRSHDNIHVRKPMFTAVCAKCGSTAYLPFQPSKF
                     QEVYCRACHHEKRGLGRKPEES"
     gene            9681..10742
                     /locus_tag="PK710_08655"
     CDS             9681..10742
                     /locus_tag="PK710_08655"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HOT09826.1"
                     /translation="MKSKRGSRAEQGKKSIPGKVTEEPREVDSSEESEEDDDAVIEAS
                     AELVGEEEDRTIGRFSEPKEDKSSAITHFDPLQAYLREVQRHPLLSPEEEHALAVRYS
                     QTGDVDAAARLVSANLRLVVKIAYEYRRAYRNIMDLIQEGNIGLMQAVKRFDPYRGVK
                     LSSYAAWWIRAYILRFVLNNWRLVKLGTTQAQRKLFFNLNKEKARLASMGIEPTSAEI
                     AKRLQVEESEVVEMDRRLAAREASLDAPIGDTDGRPVSRVEMLPSSTTGPDMQLADGQ
                     FHSLVRERMQEFRKTLHNPKDIAIFDERWLTDEPLTLQELGLRFGVTRERVRQIEARL
                     MSKLRVYLEQELGDAVSVR"
     gene            10732..>11615
                     /gene="rsmI"
                     /locus_tag="PK710_08660"
     CDS             10732..>11615
                     /gene="rsmI"
                     /locus_tag="PK710_08660"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOT09827.1"
                     /translation="MCAESRREVDLGIGAARLILVPTPIGNLGDITLRAVEVLRGADR
                     ILAEDTRRTGVLLSHLGIDRSKLHRLDANATERDLERALRWLLAGDVLAVVTDAGTPC
                     VSDPGHALVCRAVDAGVQVTALPGASAVTAAVALSGLVQGGFSFVGFVPRGQTERMDF
                     IEKLSCMAEPCVCFESPNRLAETLRAMADAMPERPIVIARELTKLHEEVIRGVVRDVA
                     LLEREWVGEVTVVLGPWAGRIEEAVSDTQVDARIDDELGRGVHTRTVAAKVAAWSGRS
                     RRDVYARVVERLEMVRKR"
BASE COUNT         2427 a         2629 c         4067 g         2492 t
ORIGIN      
        1 cccgatggcg gcaaggggca gcagcaggca tccgagccac agtgcggctg aggttccaaa
       61 ccgcatggga aaggtggcgg tcgaaatgat acgacggctt tctcggatgg gtttcgtggg
      121 tttgaccatg agcgtgtgga aaagtggtct gacgctcgat cagggtcaag ggggcagggt
      181 tatttctggg atgcgttacc acaaaacgaa catcgccacg ggcgggtggc gatgaacagt
      241 ggagctgaag ggagttgaac cctcgacctc tagagtgcga ttctagcgct ctcccaactg
      301 agctacagcc ccgagggggt atgttcgtgg cgaaacgaag cggcgctgac cttagccata
      361 gcgattcaat tgtcaaggct ttcccaaacc aaggtgcaag tctttggcta gaggctttct
      421 cgcctcctgc acacttcgga ccattgtgcg gttgacgctc gcttgcccgt ggttactttc
      481 gtccagcggc tgccacgagt cccgagggca ccgcgcaatc aaagaaaaaa catacacatt
      541 cctcaacgga acgaacgact cccacttcgt ttggggccgt cccgtccgaa cttcattatc
      601 cgtgggaggt agtattcatg ggcaagatca tcggaatcga tctgggaacc accaatagcg
      661 tcgttgctgt catggaaggc aaagacgcga aagtcatcgt taacgaagaa ggctcacggc
      721 tgaccccttc ggtcgttgcc tgggacgaca agggcgagat tctcgtcggt cagattgcga
      781 aacgtcaatc ggtgaccaac ccggaaaaca cggtgtattc atcgaagcgg ttcgtgggtc
      841 gtcgctatgt ggaggttgag caggagcgtt cgcgagttcc cttcaaagtc acgcaaggtc
      901 ccaatgggga tgcgtggttc gaggtgcggg gcaagaagct cgcgccgccc gaggtatcgg
      961 ccaaggtgtt gcaaaaactg aagaaagccg ctgaggatta ccttgggcag cccgtgacgg
     1021 aagccgtgat cacggtgcct gcttacttca acgactcgca gcgacaagcg accaaggatg
     1081 cgggaaagat agctgggctt gaggtcaaac gcatcatcaa cgagcctacg gcggctgcct
     1141 tggcgtatgg cctcgatcga aaagccgacg aaatcattgc ggtgtacgac tttggtggcg
     1201 gcacgttcga tatttcgatc ctcgaggtgg gtgataacgt ggtgcaggtc atttcgacca
     1261 acggtgacac gcatcttggt ggtgacgatc tcgacaatct ggtgatggat tggctcatca
     1321 atgagttcaa aaaagaaacg ggtattgatg tatcgaaaga caagatggtg attcaacggc
     1381 tgaaggacgc cgcggagacg gccaagatcg agctgtcttc caagatggaa acttccatca
     1441 atctgccgtt tttgaccgcg gatgcgtcgg gtccgaagca tcttcagacg acgctgtccc
     1501 gctccaaatt cgagcaaatg attcgtcctt tggtggaccg tacgctcacg cccgtgcgca
     1561 aagcgctcga ggacgccaaa aaacgcgcag aagatatcga cgaggtggtg ctcgtgggtg
     1621 gttcgacgcg cgttcctctc gtatacgact ccgtcaagca gtttttcggc aaagagccgc
     1681 ataagggtgt caacgccgac gaagtggtat cgattggcgc ggctgtccag gctggcgttc
     1741 tatcgggcga cgtcaaggac atggtgttgc tcgatgtgac gcctctttcg cttggtgtgg
     1801 agacccttgg ggcggtgatg acggtgatga ttccgcgcaa caccacgatc cccacgcaaa
     1861 agaaggagat tttctcgacg gccgcggaca accagccctc cgtggagatc catgtattgc
     1921 agggtgagcg gaccgaagca cgttacaata gaacgcttgg gcgcttccat ctagagggga
     1981 tcatgcccgc gcctcgtggt gttcccaaga tcgaagtgac cttcgatatc gacgccaacg
     2041 ggattctgtc cgtcgccgcg aaagacctgg cgacaggcaa agaccagcgc atcaccatca
     2101 cggccaactc gggtctcagc gatgaccaga tccaaaacat ggtcagcgaa gccgcggcca
     2161 acgaagcgga cgacaaacgt cgtcgcgatg aaatcgagcg ccgaaatcgt ctcgacaata
     2221 tgtgctacca gctcgaaaaa acactgaacg aaaacaagga caagcttccg tccagcgatg
     2281 tagcgacgct tcaaggcatc atcatggaag ggaagcaagc ggtggagaag caggacgatg
     2341 cgttggtgca ggaagcgctc gagaaactcg agaaggaggc ccatcgcatt gcttcggtga
     2401 tgtaccagca ggcgggtcca caagcggggg cggaaggccc tggtgccccg gcgggtgcgg
     2461 cgtctgacgg ttcttcggac gacagcaacg gctccaagaa gcgtcaggat gtcattgatg
     2521 ctgagttcga agagtccaat tgaccccttt ggggtgaccg ttctggtcac ccattttcct
     2581 ctttcgtgtt tcctgttcat cgacggctga ttcttacgat tccgctgtgg agcgagatag
     2641 actgcatggt catttctgtt tcgacggcga cgggatgaga gattatgctt tcccatccat
     2701 gagcatattc actcgcgcga tggttggtgg ggcaatggtt ctggctgctg tggttggttg
     2761 tggcagtgac gacacgcaac cgggaccttc cgcaagttcg ggcggaagtg ctggacaggg
     2821 gggaaccggt gctgcgggag tgggcgggga gggaggaagc ggggccacga gcgtgggtgg
     2881 aatgggtgga actgggggca acgccggtca aggtggggaa gcggctggca gtgttggtgg
     2941 ggcggcgggg gagacggcgg gttcaggggg cgtggcgggt tccgggggca cggttccatc
     3001 gggcggttcc ggtggaggcg cggctctcac acggatgacg atatcggggg atgccacgtg
     3061 gacggtgacg tttgatgcgc aagcgaaggc gaagggggct acggattgcc agtacacgcg
     3121 ccattacgag ggcgttcagg atcaatcgcg cccgtgggtt tgcgagcaat gcgatgtgat
     3181 atttcgcacg acggtgacga tgcctgtggg tctcaatgat tgttacaagc aggtatccga
     3241 tgtcgacccg atggatgagg agtgggtagg gtatggcaat ggcacgtggt atcgaagtta
     3301 tgggattggc gcgagcgggc aaggggacgt cgaaatcacc ggccaaagtc tgacatatac
     3361 caatgaagtc aaagacttag aggcggatat cgtcggtgct ggcaagatgg attttttggt
     3421 ggagggggcg ttcacgacct cacaggagga tggggatccg atgcatggtt gggtggcgcc
     3481 ggctcagtat gcctgtggat gggacaaggc ggacccggcc gagtacagtg gcccctattc
     3541 cttgaaggtt gggtccacgc ttccggacgg tgtgtttcgg gatgcgtgtg atgagcctta
     3601 tcggcttcat gatgcgaagg gtgcgtatct tgtcgtgttc atgtctgcga tggactgtcc
     3661 ggcttgtcgc accctggcat cgggggagtc ctcgtttttg gcggacttga aggcagaggg
     3721 gattgaagca cggacgatca ccttgatggc tccgagtttg aacgatccgt ttggagatac
     3781 gacgaaggct cagcttacgt attgggggga caagtattcg attcccacgc ccatcgttgc
     3841 ggatcgaggc tgggggataa cgatgttttt gcctatctat aatgataaat tgggttatcc
     3901 ctcgtgggtc gtcgcggatc cttctctgaa agtgttgaac tacggaacgg ggggaacttg
     3961 ggccacgatc aagaatacga ttctgaagga tgcgaagccg taagcgctgt cggagcgctt
     4021 tctctcgata gctgaagcgc gtgaagtttg gcgtacagac cgcgacgatg catgagttcg
     4081 tcgtgggagc cttgctcgac gattctgcca tggtgcagca cgatgattcg gtctgccgag
     4141 cgaatggtgg acaatcgatg ggcgatgatg agtgccgtcc ttcctttcat gagttccgcg
     4201 agcgcccctt gcaatttggc ttcggtggtg ctgtccacgc ttgcggtggc ttcatcgaga
     4261 agcacgatgg gggcatcgcg gtatagtgcc cgcgcgaaag ccacgagctg tcgttgtcct
     4321 acggaaagat tggtgccgcg ctcgtcgatg cgtgtatcga tgcctccggg gcgatcatcg
     4381 atgaggtcac cggcgccgac gcgtcgcaat ccttcttcca cgcgtttacg gtcggggatg
     4441 tgctggccca cggcgacatt ggaagcgagg gtgccggaga aaaggtaaag ttcttgtggc
     4501 accacggaga agtgctgccg caactcctgg cgatcgatac agtgaacgtc gcggccttcg
     4561 attcggatga cgccttgttg tgggtcataa aggcgcaaca gaagggaagc gatggtggat
     4621 tttccggagc cggtgggtcc gacgatggcg attttttcgc catggcgtgc ttgcaggcag
     4681 atgtcgtgca aaacaggtgt gttggggtgg tatccgaagc tgacgtggtc gaattcgaag
     4741 gcaaaggtgg gggttgcgtg cgagccatgg gttgagacga cggcatcggg ttcggggtga
     4801 tcgagcagtt cgaagacacg ttcggcaccc gccatggcgg attgaaagat ggtgtagcga
     4861 gaactcaagt cgcggatggg gacaaagaat tgttcgatgt aggcgacgaa ggcaacgagg
     4921 gtaccgaagg aaacatcggc gtggaaggtt ttgactcccg cgtaccagag gatcgaagcg
     4981 atgcagatgc tggagaccat ttcgatggtg gcatccagcg aagcttccag ggagatggcg
     5041 cgaaagtggg catttctgta ggcgaggttg atgtcatcga attcaatggc attttctttt
     5101 tcgcggcaga agctttgcac gacggcgatg ccttggactt gttcgttgag aaaggcgttg
     5161 aggcgagcga gtttggttcg gatgcttcga taagcggtgc gtgcttttct tcgtatgatt
     5221 tcgacgaaaa tgccgatgag gggcacgaga gcgaaggcta tgagggacat gcgccaatcg
     5281 agggcgagca tgaggatgac gataccgaag aggcgaagca catcgccgaa ggcattgagg
     5341 gcacccgaag cgaaaagttc gccgacggaa tcggtatcat tggtcacgcg tgtgacaagc
     5401 cgtccgatgg ggcgtcgatc gaaaaactgg aggcgttgtg tatgaaggaa ttcgaagaca
     5461 ccgcgtcgca gatcatgcat ggcgcgagcg ccaacgactt gcatggagta catttgcgcg
     5521 aaggtgagga tttgttctcc gacgatgacg acgaggagta ggaatcccgc gcgtgtgagg
     5581 gtttgtgtcg ttgcgccttc gaaggcgctt cgcatgatga ggggtcgaag cagggcgaga
     5641 gcggagatga gggcgagcat ccccatggtg agggcgaggc ctttggattg agggcgaatg
     5701 aagggccaca ggcgggctgc gagtcttctg tcgaaagcgc cgacgacttc tgattcttca
     5761 tggaagtcac ggagcacccg ttcggtgcgg gacgaagaat tcgcactcac ggtgtatctc
     5821 ccaagccgtg ggcatcgatg gaatcgattt ccccttgcag tcgttgttct tcggcgaagc
     5881 gggcgtagat gcccggctta cggatgagtt gttcgtgggt tccttgttct gcgatgcggc
     5941 cttggtcgag gacgacgata tgattgcagc gggcggcggc ggtgacgcga ctggtgatga
     6001 gaaggacggt tcttcgttgg gcttgtcgat cgatggcttt taggatggcc tgttcggttt
     6061 gggtatcgac ggcggagagg gggtcatcga gaaggaggat ggtgggttca tacagaaggg
     6121 cgcgggcgag ggcgacgcgt tgtttttgtc cgccggagag ttggaggccc cgctcgccca
     6181 cgacgctgtc cattccgtct ggaagtaatt gtatttcctc aagaatttgc gcctccgagg
     6241 cagcgaatcg aattcgctcc tggccgtcgg gggattcggg atcatcgagg gtgaaggcga
     6301 tatttcttcc cacggtggtg ctatacagga aggcgtcttg ttgggcatag ccgatggcgc
     6361 gtcgaacggt ggacaggggc aggtcgcaaa cgtcatgttc atcgagaaag acggttcctt
     6421 ttggggttgg cagcaggcga gcgagaagcg cggcgagggt ggacttaccg gagccgactt
     6481 ttcctacgat cgcgagggag tgtgccgcgg gtacgtgaaa agacacacca gtgaggacgt
     6541 tggaatcacc gaaagagaaa tgaaggtttt cgacacgcag gtcaccgtga atcgaggttg
     6601 gagcgttcag cggaccgtcg gtaatgtccg gaatggtatc gaggatttct ttgatacgag
     6661 cgtagccggc tcgtccgcgt tggacgatgg agatgacgaa accgaaggcc atgattggcc
     6721 acgtgaggcg tgcgagggcg gaccagaaag cgatgaaatc accttctgtg atggtgtgtt
     6781 ggagaacgag ccatccgccg taccagaaga cgacgatcat gccgacggcg ccgatgactc
     6841 ccatcatggg gacgagcatt ccgcgagcgc gtgcgagggc tagagctttg tcgaggtagt
     6901 gttggctagc ggtatcgaag tcgcggagtt cggctttttc gagggagaaa gcgcgaacga
     6961 tgcggacgcc gacgaggctt tcctgcacgc ggttggcgag ttggccgagg gcttgttggt
     7021 tggcggtggt acgagagaag acgacgcgtc cgtacagtct ggtgacgagg atgagcaggg
     7081 ggaagggagc gagtgcggcg agggtgagtc gcggggagat ctcgatcatg acggacaagg
     7141 cgctgacgag agcgaagagg gtattgatga cattgagaac accgaagccg agcagcaggc
     7201 gcacttgtcc gaggtcattg gtggcgcggc tcatgatttc gccggtgggg attttttgaa
     7261 agaagctgaa tcccaggcga tgcaggtggt cgatgaggtc ggcgcgaatt tggtattcgg
     7321 cgtttcggcc ggcatgaaag atggtgattc gggacaagac gcgaacagcg gcggcgatgg
     7381 ccacgagaac gagaatgaag acgacagtac gaattgtgag cgattcgttg gcggcgatag
     7441 cggcatcgac acctgttttg aacaggcgat cgcgcaggct catgagtgtt tgcaggatgg
     7501 cgagcaaaac cgttcctgcc aggtaggaag ggagctgttt tcggagttgg gcgccgaggg
     7561 agtggtggtg gctcacggtc tcgggcatgg gggcttgggt ctggttaccg tattgcacat
     7621 ttacttgccg gagcgagtga gcaagctgtt ttttcggtgg gaggattgca gggaggggcg
     7681 ttcattggaa tcgacgacgg cttcgagttg ttcggcgagc aatcgtgtgg aaaggagtcc
     7741 gagcatcacg cgggcgttgc cgcgggtatc gagttcagcg acgatgccga tcttcccgac
     7801 aaaaggccct gaaagaacgc gcacttgcgc gcctttgtcg agagtcccgc cagcggtgaa
     7861 tgcaggttgg ggttgtggcg gcgggggacg atccgcggtt ttgggtttcg gtgtgaattc
     7921 gcgggcccga gggacgggtc ggttatgggg gatcgcggct ggtggcgttg gtgtggcggt
     7981 tcgaggtgtg gcaggggagg ggggagtcgc agcggtggaa gatgtggatt tgaacagggt
     8041 tgccagggtt gggcgagagc ggttggagtc gtattcgacg cgttcacgtg ttatttcgcg
     8101 ggattgttcg atacggactt gtcggaggcg ttccgattct tgctgttgca tccgttcggc
     8161 ttccgcccgt tgcgccgcgg cttgtgagcg ttggatatgc atggcttcca gcgaccgtcc
     8221 gagttcggat acgtcgtctt gctgttgcca agccagaatt tgatacaggg ggccgagggc
     8281 gatcaaggca tcttcgagtt gttcgccaag gagttgggca tgttgcagag ccgttgcgcg
     8341 tgaaatccac catccgaacc acagagcggc gcgatgctcg tcaagggatt gggcgagttc
     8401 gcgcagcgca ggaatggagg cggaagcgat gggcaagggg ctctggccgg acacaccgaa
     8461 ttgaaactct tcgggcagtg cgaggatcgt gtcgatgatg gattgcgcgc ggtcgtcgtc
     8521 gttgagtttt gcgtggaaag cttgaagatc gatccaagca tccggatgaa ggcaggcgca
     8581 gacttcgaca tgctccgagt cgaggcgaag ggcgaggaag gcatggcgga accagggcga
     8641 ggcattgcga agggactgag cgataccggc gtgttggtcg agcagcgggt cgaggtattg
     8701 tcgttgttcg gcgggacgcc agaagatgat ccactgctca tcgacgcggt ggttgtttcg
     8761 gacgctgggg tgttcgtcgg aagcttccat atccacgggg atcccgatgg aatgaagtct
     8821 atcggcgacg gatttggccc agcccaaggc gcgttgcttg aactcgaggc gtggacggga
     8881 aaaagcattg gagcttgccc gttcggggag gtaggcgtcg aagtcgcctt cgttcaactc
     8941 gaggaatagg ggtgacattt cggctcggtg cccctgcact agacgcttcg tatggtctat
     9001 gtcaatggcg gttggcctgg atgtcaacgg gttatggttt gggaaagatg aaacagcggc
     9061 ttgaccgacg cgttgtccgt ggcaggctgg cgagcggcga tggtgctgta attcgggttc
     9121 aagggttttg agagtattat tcaatggaaa acgaagtcga tattgtgtgc gaggactgtg
     9181 gtgtgacatt cgtggttgtg ctgcatgagg agcggatgga cgccatgccg acgcggtgta
     9241 gcatgtgtcg aacccgtagg gttcagtccc aagcgcatca gcaaacaccg aagtacacgg
     9301 gtgatcggaa tgagtatcgg agtccgatgg cctgtgagta tccggaggcg ccccaacgtg
     9361 gatcgtcgtt ccgggcgggg gggcgcaagc ggtcgcacga taacattcat gttagaaaac
     9421 caatgtttac cgcggtatgc gccaagtgtg gttcgacggc gtatcttcct tttcagccta
     9481 gcaagttcca agaggtgtat tgccgggcgt gtcatcatga gaagcgagga ttgggccgaa
     9541 agcctgagga atcttgatgc tttcgtatgt ttgtaagcat tgtggcagac aggggacgag
     9601 gtaaggtgat tgcggagggt ctgggtactg gatccatttt cagcgagccg ggcgataagg
     9661 agagtcatgt gtcatcgcga atgaagagca aacgcggatc tcgcgcagaa caaggaaaaa
     9721 aatcgatacc cgggaaggta acggaggaac ctcgcgaggt tgattcctcc gaggaatcgg
     9781 aagaagacga cgacgctgtc atcgaagctt cggcggaatt ggtcggggaa gaggaggata
     9841 ggaccatcgg tcgattctcc gagcccaaag aggataagtc ttccgcgatt acgcatttcg
     9901 atccgctgca agcgtacttg cgcgaagtgc agcgtcaccc tttgctatcg cccgaggaag
     9961 agcatgcgtt agccgttcgg tattcgcaaa cgggtgacgt cgatgccgcg gcaaggctcg
    10021 tctctgccaa ccttcggctg gtggtgaaaa tcgcttacga gtatcgtcgg gcttatcgca
    10081 acatcatgga cttgatccag gaggggaaca tcggattgat gcaagcggtg aagcgcttcg
    10141 atccctaccg gggcgtgaag ctttcctcgt acgcggcctg gtggattcga gcgtatattt
    10201 tgcgctttgt gttgaacaat tggcgtttgg tgaagcttgg aacgacacaa gctcagcgaa
    10261 agctgttttt caacttgaac aaggaaaagg cgcggcttgc atcgatgggt atcgagccaa
    10321 cttccgcgga aatcgccaaa cgtcttcaag tcgaagagag cgaagtggtg gaaatggatc
    10381 gtcgtctcgc cgcacgggaa gcatcgttgg atgcacccat cggagatacc gatggtcgtc
    10441 ccgtctctcg ggtggagatg ttgccgtcct cgaccactgg gcccgatatg cagcttgcgg
    10501 atggccaatt tcattcgttg gtacgcgagc ggatgcagga gtttcgaaag acgcttcaca
    10561 atccgaagga cattgcgatt ttcgatgagc gatggttgac cgacgagcct ttgacgcttc
    10621 aggagttggg tcttcgtttt ggtgtgacgc gggaacgtgt tcgtcaaatc gaagcgcggt
    10681 tgatgagcaa gctgcgtgtc tatctcgagc aagagttggg agatgcggtg agtgtgcgct
    10741 gaatcgcggc gtgaagtcga tcttgggatt ggcgcggcaa gactgattct ggtgccgaca
    10801 cccattggaa atttgggcga tatcacgttg cgggcggtcg aggtgcttcg aggggcggat
    10861 cgcatcctag cggaggacac gcgacgaaca ggtgtattgc tgtcgcatct gggcatcgac
    10921 aggagcaagc ttcatcgatt ggatgccaac gcgacggagc gagatttgga gcgtgcgctg
    10981 cggtggttgc tggcaggtga tgtgttggct gtggtgaccg atgcggggac tccttgtgtg
    11041 agcgatcccg ggcatgcgtt ggtttgtcga gcggtggatg ctggggtaca ggtgacggcg
    11101 ttgccggggg catcggcggt gacggcggcg gtggcgttat cggggttggt gcagggaggg
    11161 ttttcgtttg tgggattcgt tccgcggggt cagaccgaac gtatggattt tattgagaaa
    11221 ttatcgtgta tggcggagcc ttgtgtatgc ttcgagtcac cgaatcggtt ggcggagacg
    11281 ctcagggcta tggcggatgc gatgccggag cgtccgattg tgatcgctcg ggaattgacg
    11341 aagctgcatg aggaggtgat tcgaggtgtt gtgcgagacg tggccctgct ggagcgagag
    11401 tgggtgggag aagtgacggt ggtgttgggg ccatgggctg ggcgaatcga ggaggcggtg
    11461 agtgatacgc aagtggatgc gcggattgat gatgaacttg ggcgtggtgt gcatacgcgg
    11521 acggtggcgg cgaaagtagc ggcgtggagt gggcgaagcc gtcgcgatgt gtatgcgcgt
    11581 gtggtggagc ggttggaaat ggtgaggaag aggaa
//
LOCUS       DAOSOR010000120        11602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5535_length_11602_cov_4.397506, whole genome shotgun sequence.
ACCESSION   DAOSOR010000120 DAOSOR010000000
VERSION     DAOSOR010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11602
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5535_length_11602_cov_4.397506"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /gene="nrfD"
                     /locus_tag="PK710_08665"
     CDS             complement(<1..621)
                     /gene="nrfD"
                     /locus_tag="PK710_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HOT09828.1"
                     /translation="MASEISPSETGSHWSSYPKFLYRLLVLATDGSLLFYAWMTGLTA
                     VALVGANAWAQQVSQGMGLTHMSDHVSWGLYIANFTFVVGVAAGGVMMVIPAYVYHDR
                     KMHDVVIFGEMLSIAAILMAQLFVLVDLGRPDRFWHLVPGLGQFNWPLSMLSWDVLVL
                     NGYLVLNLHICGYLLYMRHLRREPNPKWYVPFVFISIVWAISIHTVT"
     gene            complement(630..1553)
                     /locus_tag="PK710_08670"
     CDS             complement(630..1553)
                     /locus_tag="PK710_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOT09829.1"
                     /translation="MSKDDNNKSRFSRRDAMKVAGGAVGVAAFAQAMAPIMGFADGIS
                     MQEMLQDHYKRLTDDDKSRIFKRLEEEARRKYGAEVRIRDPQPMENVKFGYALNLSVC
                     IGCRQCVKACRIENNHNRKNRGSYIRVFEMDKASMELAQASSSYEHPVRVTGRRYMPI
                     QCHQCDKPPCVDVCPVKATWKEPDGIVVIDYNWCIGCRYCEAACPYHARFFNWTDPEI
                     PAEEVTADQGYLSNRIRPRGVMEKCTFCLHRTREGRLPACLEACPAGARVFGNLLDPN
                     SEIRWVLENKRVYVLKEEQGTQPSFYYFFSE"
     gene            complement(1561..2184)
                     /locus_tag="PK710_08675"
     CDS             complement(1561..2184)
                     /locus_tag="PK710_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09830.1"
                     /translation="MLSLAPRMLETLLRTLAILVVVPWSLCGCEQARADDTKSAKPAA
                     SPSASAGLFPVSIHRPYPELGMNTGVAGPDGKAARTPCRTCHGFVEPKEKYAQANHLE
                     AFHKGVEIQHGGQSCRTCHSVPGFDSFNLAGGERISYDEVVRLCGQCHAQRLVEYQRG
                     AHGGMTGYWDLRRGPRSRNHCLDCHNPHAPKIPQMVPAPALVNRTFE"
     gene            2315..2581
                     /locus_tag="PK710_08680"
     CDS             2315..2581
                     /locus_tag="PK710_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09831.1"
                     /translation="MAVSSLLLWLDPDANDPDRTLESLAAQPHLTLGTPVQLRVPAVL
                     DTPTIQQDIDTVAQLLALPGIAHVDVLGVFFDDTQTVSPGGSDV"
     gene            2574..4985
                     /locus_tag="PK710_08685"
     CDS             2574..4985
                     /locus_tag="PK710_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOT09832.1"
                     /translation="MSDPVSRRTFVRSAALGVASAAAMTTIHRSAETAPPVDPRTTMP
                     DLDWQKAPCRFCGTGCHVLVGVKNGKVAAIAGDPEADVNKGLLCVKGYHVGLILYGAD
                     RLTKPLLRKNGQLVPIGWDEALDILADRVMKDPKGFAFYGSGQWTIPEGFAAQKFMKG
                     GLGNNHIDPNARLCMASAVTGFLSTYGVDEPAGCYQDLDECNVLILWGNNPAEMHPVL
                     FSRLIDRRSRGDDIRLIDICTRRTRSSLHAQNVLIFKPHTDLAIANGIAHLLLERGTY
                     DKEFVEKHCAFRADSEPRELIGKAISFEEYRKSLEPYTPEHVQEISGVPAKDIRMLAD
                     LFGDRSLRITSLWCMGMNQHTQGTAINSLVHGIHLLSGHFGKPGDAPTSLTGQPSACG
                     TVREVGTLSHALPGGREVANDEHRKQAEEFWKLPPGRIQKTPGYHTIKMWEQFCKPTD
                     QGGDIHTIWVQVTNPGQTLPNLNKLFRAKEKLQDKFLVVSDVYPTATTQLADMILPSA
                     LWVEKNGMFGNSERRTQQWFRMVKPPGDARDDCWQTIALARRLYDRGHPGMKDKDGNF
                     LFDVRDSNGKPVPVWDFKHYYDVNVDKHLFEEYRLFTRMKHKDLAPYDEYVKARGLRW
                     PVVQQADGSWRETRFRFAEFDDPYVKKGQGIEFYHSTSKDGRAQIWFRPYVPPPESPS
                     KDFPFWLCTGRVLEHWHTGTMTRRVPQLLRAMPEAYVEIHPDDARDAGIHNGDLVKIS
                     TRRGSIDVRAWIGGRGSPPRGLLFVPFFDENKLINLVTLEDFDFFSKQTDYKKCAASI
                     ARVTS"
     gene            4993..5730
                     /locus_tag="PK710_08690"
     CDS             4993..5730
                     /locus_tag="PK710_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09833.1"
                     /translation="MAKPPSFAPYPRAVNVALAMVIGVAVVGYAVGIRPAQVQAIFPA
                     LAVSPSGAIPGQTYLDWRQRRDGPNAVVRSNLGDLRAALPAVSSPVVRTPENKREALE
                     TRANIRAYEGAPPTIPHPIDEQNPGSCLACHRDGLVVEGRVARAISHATYTNCTQCHV
                     TMEPRFEKPPAPDNAFVGYRLDRKREQAWQGAPPVIPHSVWMRDRCESCHGVAGLPGL
                     RTTHPERGQCTQCHVSRTEYSPPWAAR"
     gene            complement(6063..7040)
                     /gene="cysK"
                     /locus_tag="PK710_08695"
     CDS             complement(6063..7040)
                     /gene="cysK"
                     /locus_tag="PK710_08695"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOT09834.1"
                     /translation="MPKYFDDNSQSIGRTPLVRLQRMTAGCSATVLAKIEGRNPGYSV
                     KCRIGASMVWDAEKRGLLRAGKQIVEPTSGNTGIALALVAAARGYPLTLTMPETMSVE
                     RRKVLAAFGAQLILTEGSLGMKGAIARAESIAASEPDLYFMPQQFKNPANPGIHETTT
                     GPEIWEDTDGLIDVLVSGVGTGGTITGVSRHIKKTRNKPILSVAVEPAQSPVLSQTLA
                     GKEVKPGPHRIQGIGAGFVPDTLDLSLVDQVETVTDDEAIEFARRLAKEEGILSGVSC
                     GAAAAAAIRLAKNPAFAGKTIVVVLPDSGERYLSGPLFEGVFDAEGRAL"
     gene            7166..7723
                     /locus_tag="PK710_08700"
     CDS             7166..7723
                     /locus_tag="PK710_08700"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOT09835.1"
                     /translation="MDEINQQVAQRLKNLRMAAGLSLQDLALRSGVSRAMLSQIETLK
                     TNPTIAILWKVAEGLGIPFAELIDTAVASPTRLSRLADARFLSSADQSFRSRPLLAHV
                     PGHSVELYEIELAPGAVEQAAPHPPATHEQVIVTSGRLSLRVGSDIFTLGPRDAVLFR
                     ADVHHEYRTVGTKPFHGLSLILYGH"
     gene            complement(7778..9166)
                     /gene="der"
                     /locus_tag="PK710_08705"
     CDS             complement(7778..9166)
                     /gene="der"
                     /locus_tag="PK710_08705"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOT09836.1"
                     /translation="MSARKPRDKGEERNRIPHRANALVAIVGKPNVGKSALFNRLVGR
                     RQAIVHDEPGVTRDRHYAEAWSLGRRYTVVDTGGFDPESDDPMRQGISRQVQVAIEQA
                     DLIILVMDGVQGPTEADRRAVGLLRRSDRPVLFIANKVDSEKSVLDANELFELGIDSL
                     ISVSALHGRGFHELEDALVQALPAVEEPTDSQVEDVIRVAVVGRPNAGKSSLINRLAG
                     EDRVLVDSRPGTTRDPVDTLLQRDGKQFLFVDTAGIRRKSRVVKERDTVEVVSVLRAM
                     RAMEEAEIVVLMCDAALGVAEQDAKILGLAEQRGRAVVVALNKMDQLTRPQLADAEKK
                     AKEKLSFAPWIPYVPTSALTGRGTQALLRKIVAIRDNFYRRVPTAELNRFFDSVLQTH
                     PPPTQGGKAPRLYYVTQAETAPPHFVVMSNAPESIHFSYQRYVKNQLRGAFDFEGVPV
                     RVAYRARRRRED"
     gene            complement(9163..10116)
                     /gene="era"
                     /locus_tag="PK710_08710"
     CDS             complement(9163..10116)
                     /gene="era"
                     /locus_tag="PK710_08710"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOT09837.1"
                     /translation="MKPPAEQRSNRSGTAAIVGRANVGKSTLLNTALRERLAIVTPTP
                     QTTRDRILGVLRHGDAQIALLDTPGLHRPRSRLGRRMNTMARDAAREADVLVFVTAIP
                     KNVSSDLAPAGRDVEILQQVDPESRAILVINKIDLLGKNRSLLLPLIEQFSKLRSFSA
                     IVPISALRDDGVNLLLDEIASLLPERGPAYEEDFLTDRPVRFFASEYVREQVILATKQ
                     EIPHHVAVTIERFEEGVHVTHIDATVRCAREGQRKIIIGKGGERLKGIGVAARQRIES
                     LVGRQVHLSLWVTIDQGWPESAHALDSLGYVSVSGDDEAVS"
     gene            complement(10176..>11602)
                     /locus_tag="PK710_08715"
     CDS             complement(10176..>11602)
                     /locus_tag="PK710_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HOT09838.1"
                     /translation="CPTPGEPCHPTVRGDGIREGDEACDDGNDVIGDGCNPFCEVEPD
                     CSKGGACVSACGDGMMLPGDDEECDDGNTKSGDGCSADCKIEIGFECKTVKTDLPDEL
                     EVPATFRDFIALPAGAGVRHPDFEHFSGAAATPGLVNSSLGVDGKPQYTGICEEGNIV
                     GPCPYGPQTSSKAAFDQWYRNDPSVNKTEVAKIVLTRQPNGSYFFPDAAFFPLDGKGW
                     VAAGADNASGGHNFGCTSELRTWFEFKGGESLEFSGDDDVWVFINRTLVVDLGGLHSQ
                     RPGDVTLDGPKAQQLQLQTGKIYEMALFHAERHTTASNFNLTLTGFVKAKSQCQSLCG
                     DGIVVGDEECDDGVNDGSWGSCTADCRLGPYCGDGAVQTPYEECDDGINLTTYSPTGE
                     PGCAPGCKYGAYCGDGKVDSLFGEQCDDGINKGGYGGCTPSCRLDARCGDGVLQAQHG
                     EECDDGNNVSGDGCSSDCKREGPK"
BASE COUNT         2594 a         3647 c         2954 g         2407 t
ORIGIN      
        1 cgtcaccgta tgaatcgaaa tcgcccacac gatcgaaatg aaaacgaaag gaacgtacca
       61 cttcggattc ggttctcgtc gcaaatggcg catgtacagc aagtagccgc aaatatgcag
      121 gttgagcacc agatagccgt tgagcaccaa cacgtcccag gaaagcatcg acaacggcca
      181 gttgaattgt ccaaggcccg gaaccaaatg ccagaaacgg tccggtcttc ccagatccac
      241 caacacgaat aactgcgcca tcaaaatcgc ggcaatcgac agcatctcgc cgaaaatcac
      301 cacatcgtgc attttgcggt cgtggtagac gtacgctgga atcaccatca tcacaccccc
      361 cgccgccact cccaccacga acgtgaagtt cgcgatatac agaccccacg acacatgatc
      421 ggacatgtgc gtcaaaccca tgccctggga gacttgctga gcccaggcat tggcccccac
      481 caaagccacc gcggtcaagc cagtcatcca agcgtaaaac agcaaggacc cgtccgtggc
      541 aagcaccaac agacggtaca aaaacttcgg atagctgctc cagtgcgaac ctgtctcgga
      601 aggagagatc tcggaagcca tcatagccct cattcggaaa agaaatagta gaagctgggc
      661 tgcgttccct gctcttcctt gagcacatac acgcgcttgt tctcgaggac ccatcggatc
      721 tccgagttcg gatcgagcaa gttaccaaac acgcgagcgc ccgcgggaca ggcctcgaga
      781 cacgcgggca ggcgcccttc ccgtgtgcga tgcaagcaaa acgtacattt ttccatcacg
      841 ccccttggtc gaatccgatt ggacaaatat ccctgatcgg ccgtgacttc ctccgcggga
      901 atctccggat ccgtccaatt gaaaaaccga gcgtgatacg gacacgccgc ctcgcagtac
      961 cgacagccaa tgcaccaatt gtagtcaatg accacaatcc catccggctc cttccacgtc
     1021 gctttgaccg gacacacatc cacacagggc ggcttgtcac attgatgaca ctgaatcggc
     1081 atatagcgtc gccccgtcac tcgaacggga tgctcgtacg aactcgacgc ctgtgccaac
     1141 tccatgctcg ccttgtccat ttcgaatacg cgaatgtacg agccgcgatt ctttcgattg
     1201 tggttgttct cgatccgaca ggccttcaca cactgccgac aaccaatgca aacactgaga
     1261 ttgagcgcat acccgaactt gacgttttcc atcggctgcg gatcccgaat ccgtacctcc
     1321 gcaccgtatt ttcgtcgcgc ctcctcctct aacctcttga aaatacgtga tttatcatca
     1381 tccgtcagac gtttataatg gtcctggagc atctcctgca tcgagatgcc gtcggcaaaa
     1441 cccatgatcg gagccatggc ctgcgcaaac gccgccactc ccacggcgcc accggctacc
     1501 ttcatggcgt ctcgacgaga aaaccgagac ttattgttat cgtccttcga catggactcc
     1561 tcactcaaac gtccggttga cgagcgcggg cgctggcacc atttggggta tcttcggcgc
     1621 atgcggattg tggcaatcga ggcaatgatt gcgagaccgc ggaccgcgac gaagatccca
     1681 ataccccgtc atcccaccat gggcaccccg ttggtactcg accaaccgct gcgcatggca
     1741 ttgcccacac aaacgcacca cttcgtcata agaaatccgc tcaccccccg cgagattgaa
     1801 gctatcgaac ccgggcacgg aatgacacgt ccggcaggac tggccgccgt gctgaatctc
     1861 cacacccttg tggaaggcct cgagatgatt ggcctgcgca tatttttctt tcggctccac
     1921 aaagccatga cacgtgcggc atggcgtgcg ggcagccttg ccatcagggc ctgccacccc
     1981 agtgttcatc cccaattccg gatagggcct gtgaatgctg accggaaaaa gccccgccga
     2041 agcagaaggg cttgccgcag gctttgccga cttggtgtca tcggcacgcg cctgttcaca
     2101 gccacataac gaccacggca ccaccacgag gatggcaagc gtgcgcagca gagtctcgag
     2161 cattctcgga gccaacgaga gcatgggaac tctcccgaca aggttgaaag ataggaccgg
     2221 ccaggaggct agcgcgccca gccatccctg ttcaaggata aaaaccgata tagcagtctt
     2281 gaattcttct ttcgcaacga ctagcgttcc ccccatggcc gtcagctctt tacttctctg
     2341 gctcgatccg gatgccaacg atcccgatcg cacgctcgag agccttgctg cacagcctca
     2401 tcttacgctc ggcacgcccg ttcaacttcg cgtccctgcc gtgctcgaca cgcctaccat
     2461 ccaacaggac atcgataccg tggcccagct cctcgctctg ccaggtatcg cccatgtcga
     2521 tgtgctcgga gtcttcttcg acgatacgca aaccgtctcc cccggaggct cggatgtctg
     2581 atcctgtctc acgacgtaca ttcgttcggt ccgcggcgct tggtgtcgcg tcggccgccg
     2641 cgatgaccac gattcaccgt tcggccgaaa ccgccccccc ggtcgatcct cgcaccacca
     2701 tgccagactt ggactggcaa aaagctcctt gccgcttttg cggcacggga tgtcatgtgc
     2761 tcgtcggagt caaaaacggc aaagtcgccg ccattgcggg agatcccgag gcagatgtca
     2821 acaaaggatt gctctgcgtc aaaggctatc atgtgggttt gattttgtat ggggcggacc
     2881 ggctgaccaa accgttgctt cgaaaaaatg gccagctcgt gcccatcgga tgggatgagg
     2941 cgctcgatat cctcgcggac cgtgtgatga aagaccccaa gggtttcgcc ttttatggca
     3001 gcggccagtg gaccatcccc gaaggattcg cggcgcaaaa attcatgaaa ggcggcctgg
     3061 gcaacaacca tatcgacccc aacgcacggc tatgcatggc ctcggccgtc accggatttc
     3121 tttccaccta cggtgtcgat gaaccggccg gatgttacca ggatctcgat gaatgcaatg
     3181 tcctcatcct atgggggaat aacccggcgg aaatgcatcc cgtgctgttt tctcgcctca
     3241 tcgatcgacg ttccagaggc gacgacatcc gtctcatcga tatctgcaca cggcggaccc
     3301 gatccagcct gcatgcccaa aatgtcctta ttttcaaacc acatacggac cttgccatcg
     3361 ccaatggcat cgctcatctc ttgctcgaac gcggcactta cgacaaggaa ttcgtcgaga
     3421 agcattgcgc gtttcgcgcc gacagcgagc cgcgagaact gatcggcaaa gccatttcct
     3481 tcgaagaata tcgaaagagc ctcgagccct acacccctga gcatgtgcaa gagatttcgg
     3541 gtgtacccgc caaggatatc cgaatgctcg cggacttatt cggcgaccgc tccctgcgaa
     3601 tcacaagcct ttggtgcatg ggcatgaacc agcataccca aggaacggcc atcaattccc
     3661 tggtgcatgg cattcatttg ctcagcggtc attttggcaa acctggcgac gcgcccacca
     3721 gtttgacggg tcagccttct gcttgcggaa ccgtgcgaga agtcggcacc ctgtcacatg
     3781 ctctaccggg agggcgggag gtagccaacg acgagcatcg aaaacaggct gaggaattct
     3841 ggaaactacc gccaggacgc atccagaaaa cgcctggata ccacaccatc aagatgtggg
     3901 agcagttctg caaacccacc gaccagggcg gcgatattca taccatctgg gttcaggtca
     3961 ccaaccccgg acaaaccctg cccaacctga acaaactttt ccgggccaaa gaaaaactcc
     4021 aggacaaatt cctcgtcgta tccgacgttt accccaccgc cacgacacaa ctcgccgata
     4081 tgatcctccc ttcggcattg tgggtcgaaa aaaatgggat gttcggcaac tccgaacgac
     4141 gcacccaaca atggttccga atggtaaaac caccaggcga tgctcgcgac gattgctggc
     4201 aaaccatcgc cctggctcgt cgtctctacg accgcggaca tccgggtatg aaggataaag
     4261 acggcaattt cctattcgat gtccgggact cgaacggaaa acccgtgccc gtctgggatt
     4321 tcaaacacta ctacgacgtc aatgtggaca agcatctttt tgaggaatat cggctattta
     4381 cacgtatgaa gcacaaggat ctcgcgccgt acgacgaata cgtcaaggcg cgggggcttc
     4441 gatggccggt cgttcagcag gcggacggaa gctggcgtga gactcgattc cgatttgcgg
     4501 aattcgacga tccgtacgtg aaaaaagggc aaggaatcga gttttatcac tcgacctcga
     4561 aggatgggcg ggcgcagatt tggttccgtc cttatgttcc tcctccggaa tcgccgagca
     4621 aagattttcc tttctggctt tgcacgggcc gcgtgttgga gcattggcat acgggaacga
     4681 tgacgcgacg ggtaccgcaa ctgcttcgcg cgatgccgga ggcgtacgtg gagattcacc
     4741 ccgacgatgc acgggatgcg gggattcata atggcgatct ggtgaagatc agcacgcgac
     4801 ggggcagtat cgacgttcgg gcttggattg gcggcagggg ttctccccct cggggtctgc
     4861 ttttcgtccc cttcttcgat gaaaacaagc tgatcaacct tgtgaccctc gaggatttcg
     4921 actttttctc caagcagacg gattataaaa agtgcgcggc gagcattgct cgcgtgacct
     4981 cctgaggcga gtatggctaa acctccttct tttgccccct accctcgcgc ggtcaacgtg
     5041 gcgctcgcga tggtgattgg tgtcgcggtg gtgggctatg ccgtcggaat ccggcctgca
     5101 caagtgcaag caatctttcc agcgctcgcg gtaagccctt cgggggccat ccctgggcag
     5161 acctacctgg attggcgaca gcgtcgggat gggcccaacg cggtcgttcg cagcaatctg
     5221 ggggatttgc gggcggcatt gccagcggta tcgtcgccgg tggtccgcac cccggaaaac
     5281 aagcgagaag ccttagaaac ccgtgcaaat atcagggctt acgagggcgc tccgccaacc
     5341 attccccacc ctatcgatga gcagaacccg ggctcgtgcc tggcctgtca ccgcgatggg
     5401 ctggtcgtgg aaggacgtgt ggcacgcgcc atttcccatg caacctacac gaattgcacg
     5461 caatgccatg tgaccatgga gccgcgtttc gaaaaaccac ccgcccccga caacgcgttt
     5521 gtggggtatc gcctggacag aaagcgcgaa caggcctggc aaggcgctcc tcccgtcatt
     5581 ccccattcgg tgtggatgcg ggatcgttgt gagagttgtc acggcgtggc gggccttccc
     5641 gggcttcgaa cgacgcaccc cgagcgggga caatgcacgc aatgccacgt gtcgcggacg
     5701 gaatactctc ctccctgggc ggcgcgctaa ccaatccctt ccccaatgct gttgggctgt
     5761 tgtgcaggcg tagaaagcag gcgctttcca tgactgcttg tgcgtatcga agaaaagcgc
     5821 tattttgatg ccacaaaagg ggcaacgccc gggacgcggg ggggctattc gtctaccggg
     5881 cgttgaatag attgtgcaac gtcagggact gaggggggcg tttgtcacga ccaacgttgc
     5941 tgactgctat tgttgcaccc actgtgccaa atagagttat tgtaaaatca aatagttaga
     6001 aggtgtgacg gaagagtcaa ttccaggagt gcaaattcta ccattcgaga tcgccaatca
     6061 tcctacagcg ccctaccttc cgcatcgaaa acaccttcga atagcggccc gctcaaatat
     6121 cgctcgccgg aatcgggtaa caccacgacg atcgtttttc ccgcaaacgc gggatttttc
     6181 gccagacgga tggcggcggc cgcggcggca ccgcaagaaa cacctgaaag aatgccttct
     6241 tctttggcga ggcgacgagc aaactcgatg gcttcgtcgt cggtgacagt ttccacctga
     6301 tcgacgagcg acaggtcgag ggtgtctggc acgaatccgg ctccaattcc ctggatgcga
     6361 tgggggccag gcttgacctc ttttccggcc agggtttggg aaagaacggg gctttgcgcg
     6421 ggttccaccg ctaccgatag aatcggtttg ttccgcgttt ttttgatatg gcgcgatacg
     6481 ccggtgatgg tgcctccggt gcccaccccc gaaacgagaa cgtcgatcaa accatccgta
     6541 tcttcccaaa tttctggccc ggtcgttgtc tcgtgaatcc ctgggtttgc agggtttttg
     6601 aattgctgag gcatgaaata caaatccggc tcgctggcgg caatgctttc cgcacgcgca
     6661 atcgctccct tcatgcccaa cgatccctcc gtcaatatca attgagcacc aaaagccgcc
     6721 aacactttcc gtcgctccac actcatggtc tcaggcatcg tcaacgtgag cggatagccc
     6781 cgcgccgccg ccaccagcgc tagcgcaatg cccgtgttgc cactcgtcgg ttccacgatc
     6841 tgcttgccag cacgaagcaa accacgcttt tccgcgtccc ataccatcga cgcaccaatc
     6901 ctacacttga cagaatatcc tgggtttcta ccttcgatct tcgccaaaac cgtggccgaa
     6961 catccagccg tcatccgttg aagccgaacc agcggcgtgc gccctatcga ctgagaatta
     7021 tcatcgaaat acttcggcat gagaaggtct ccaagaattg cgaaaagggt tcatccatcc
     7081 cttgcttcga tggctccgag acgtcaatat aacggacgaa ttcaatacgt caacgatttg
     7141 tttgatataa catacaggac gacgcgtgga cgaaatcaac caacaagtgg cgcagcgcct
     7201 gaaaaacctg agaatggccg ccgggttgag cttgcaagac ctggccctgc gttcgggcgt
     7261 cagccgcgcc atgctcagcc aaatcgaaac cctcaaaacc aaccccacca tcgccatcct
     7321 ctggaaagtc gccgaaggcc tcggtatccc cttcgccgaa ttgatcgata ccgccgtcgc
     7381 ttcccctacg cgcctcagcc gattggccga tgcacgcttc ctttcgtcgg ccgatcaatc
     7441 gtttcgaagt cgcccccttc tcgctcacgt ccccggtcac tccgtcgaat tatatgaaat
     7501 cgaattggcg ccaggagccg tggaacaagc cgcccctcat cctcccgcca cccacgaaca
     7561 agtcatcgtc acctctggtc gcctgtctct tcgcgtcggg tcggacatct tcaccctcgg
     7621 cccccgtgat gccgtccttt tccgcgccga tgtccaccac gaataccgaa ccgtgggaac
     7681 caaacccttc catgggctgt cgttgatcct ttacggtcac tgagcacgac ttgtggtcgc
     7741 gcgtttcgca aagcgaatcg cagcgcctcg gttcgcatca atcctcacga cgacgtcttg
     7801 ctcgataggc cacccgcacc ggaacgcctt cgaagtcgaa tgctccacgc aactggtttt
     7861 tgacgtaccg ttgatagctg aagtgaatcg attcaggcgc attggacatg accacgaaat
     7921 gcggaggagc cgtttccgct tgtgtgacgt agtacaggcg cggtgctttg cccccttgcg
     7981 tgggcggcgg atgagtctgc aacacgctat cgaaaaaccg attcaattca gccgtgggaa
     8041 cgcgtcgata aaaattgtct cgaatcgcga cgatcttccg aagcaacgct tgcgttcccc
     8101 gacctgtcag cgcactggtc ggcacataag gaatccacgg cgcgaaggaa agtttttctt
     8161 tcgctttttt ttccgcatcc gcaagctgtg gacgcgtgag ttggtccatc ttgttgaggg
     8221 ccactacaac ggctcgcccc cgctgctccg caagaccaag aattttcgcg tcttgctccg
     8281 caaccccgag agccgcatcg cacatcaaca caacgatctc ggcctcctcc atcgcccgca
     8341 tggctcgcaa tacactcacc acttccaccg tatctcgttc tttgacgaca cggctttttc
     8401 gacgtattcc cgccgtatcc acgaacaaaa actgctttcc atcccgttgc agcagcgtat
     8461 cgaccggatc tcgcgtggtt cccggacgag aatcgacaag aacccggtct tcccccgcaa
     8521 gacgattgat gagagaggat ttgcccgcgt tcggtcttcc caccacggca acccgaatga
     8581 catcttccac ctgtgaatcc gtgggctctt cgactgcagg caaagcctgc accaatgcat
     8641 cctccagttc gtgaaaacct cgaccatgca aagcactcac agaaatcaat gaatcgatcc
     8701 ccagttcgaa taactcattg gcatcgagaa ccgatttttc ggaatccacc ttgttggcaa
     8761 tgaatagaac cggcctgtcg cttcgtcgca gcaatcctac agcgcgacga tccgcttcgg
     8821 tcgggccttg gactccatcc atcaccaata tgatgagatc cgcttgctcg atcgccacct
     8881 gcacttgccg cgatattccc tgacgcatgg gatcatcgct ttcggggtcg aaaccacccg
     8941 tgtcgaccac ggtatagcga cgacccaacg accacgcttc ggcgtaatga cggtcgcgag
     9001 tcacaccagg ctcatcgtga acgatggctt gccgtcgacc gaccaaacga ttgaaaagcg
     9061 cggacttacc cacattgggt tttcccacga tcgccaccag agcgttggct cgatgaggga
     9121 tgcgatttct ttcctcaccc ttgtcacggg gttttcttgc gctcatgaaa ccgcctcgtc
     9181 atctccggag acggatacat agccgaggga atccaacgcg tgcgcggatt ccggccagcc
     9241 ctggtcgatg gtcacccaaa ggctcaagtg aacctggcgt ccgacgagcg attcgatgcg
     9301 ttgacgcgcg gcaactccaa ttcctttgag ccgctcgccg cctttgccaa tgatgatttt
     9361 tcgctgtcct tcgcgggcac aacgcacggt cgcgtcgata tgggtgacgt ggacaccttc
     9421 ttcgaaccgc tcgatcgtca cagccacatg atggggaatc tcttgtttcg ttgcgagaat
     9481 cacctgctcg cgaacgtatt cggaagcaaa aaagcgaacg ggacgatccg tcagaaaatc
     9541 ctcctcgtag gccggtcccc gctcgggaag aagggaagcg atttcatcga gcaacaagtt
     9601 gacaccgtca tcgcgtaacg cgctgatggg aacgatcgcc gaaaacgaac gtaattttga
     9661 aaactgttcg atgagaggca acagcaacga acggttcttc ccgagcagat caattttatt
     9721 gatgacgaga atcgcccggc tttccggatc tacctgctgc aggatctcca cgtccctgcc
     9781 cgcaggagcc aaatccgacg aaacattttt ggggatggcc gtcacgaaca cgagcacgtc
     9841 ggcctccctg gccgcatctc gggccatcgt gttcatacgt cgtccgagtc ggctgcgcgg
     9901 acgatgcagt cctggcgtat cgagcagggc gatttgcgca tcgccatgac gaagcacccc
     9961 aagaatccgg tctcgcgtgg tttgcggcgt cggggtaacg attgccaaac gttcacgaag
    10021 agcggtgttg agcagcgtgc tttttcccac gttcgcgcga cccacgatgg cggcggtccc
    10081 cgaacggttg gaacgctgtt ccgcaggagg tttcatgatg agacttccta tagcagcacc
    10141 cggaatttcc agggaacgga ttccaacgtc agcgcttact ttggcccttc tctcttacaa
    10201 tccgaagaac aaccatcccc agatacgttg ttcccatcat cgcactcctc accatgctga
    10261 gcctgcaata ccccatcacc acatcgagca tccagccgac aagacggcgt acagccaccg
    10321 tacccgcctt tgttgatacc atcgtcgcac tgctcaccaa acaaactatc cacctttcca
    10381 tccccgcagt aggcgccata cttacatcca ggtgcacagc caggttcgcc cgtgggtgaa
    10441 tacgtggtca ggttgatccc atcgtcacat tcctcatacg gcgtctgaac cgcaccgtcg
    10501 ccacaatagg ggcccaaccg acagtccgcc gtgcaggagc cccaggaccc atcgttcacg
    10561 ccatcgtcgc actcctcatc gcccacgacg atcccatctc cgcagagcga ttggcattgg
    10621 ctcttggcct tcacgaaccc cgtgagcgtc aggttgaagt tcgaggccgt cgtatgacgt
    10681 tccgcatgaa acaaggccat ttcatagatt tttccagttt gcaattgcag ttgctgtgcc
    10741 ttcggcccat cgagggtgac atccccgggg cgctgggaat gaagcccacc caggtcgacc
    10801 acaagcgttc gattgatgaa tacccaaacg tcatcatcgc ccgaaaactc aagtgattcg
    10861 ccccctttga attcaaacca cgttcgcaat tcactcgtgc atccgaagtt atgaccaccg
    10921 ctcgcgttat ccgcgcccgc cgccacccag cccttcccat cgagcgggaa aaacgcggca
    10981 tccggaaaga aatacgaacc attgggctga cgcgtcaaaa cgatcttcgc tacttccgtc
    11041 ttgttcacgg aaggatcatt tctgtaccac tgatcaaacg ccgccttgct cgacgtctgc
    11101 ggaccataag gacaaggccc cacgatattt ccttcttcgc aaatccccgt gtactgaggc
    11161 ttgccatcga ccccaaggga tgagttcaca agtcctggtg ttgcggcagc cccactaaag
    11221 tgctcgaaat ccggatgccg aacacctgcc cctgccggaa gcgcaatgaa gtcccgaaac
    11281 gtcgccggta cctccaattc gtccggcaaa tccgtcttga ccgtcttgca ctcaaatccg
    11341 atttcaatct tgcagtccgc ggagcaacca tctccgcttt tcgtgttgcc gtcgtcacat
    11401 tcctcatcat cgcccggcag catcatccca tccccacacg cggaaacgca cgctccgccc
    11461 ttactacaat ccggctccac ctcgcaaaat ggattgcagc catcgccaat cacatcgttg
    11521 ccgtcatcac aagcttcgtc cccttcgcga attccgtctc cacgcaccgt gggatgacac
    11581 ggctcccccg gcgttggaca gg
//
LOCUS       DAOSOR010000121        11579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5551_length_11579_cov_4.513016, whole genome shotgun sequence.
ACCESSION   DAOSOR010000121 DAOSOR010000000
VERSION     DAOSOR010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11579
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5551_length_11579_cov_4.513016"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PK710_08720"
     CDS             <1..313
                     /locus_tag="PK710_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09839.1"
                     /translation="RLEVSDRQLDALRARASDIEAVATGAVQRTEEYVLQALQSDSTL
                     AFRFWSRRALDAIGGIAWAEVRAVCTHLRRMIGASFRLDFKRRECFLAELMAAAVPVA
                     G"
     gene            complement(407..814)
                     /locus_tag="PK710_08725"
     CDS             complement(407..814)
                     /locus_tag="PK710_08725"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOT09840.1"
                     /translation="MQQQLEKVAHAHPARKGLAVWTFPVGVNCALGNLIEERAGLTVQ
                     LKQRVRLDGVAPGLLYNYLLARGLCLEDVVSPRQFEDLVAEVFRAEGWDVYQTKQTRD
                     GGKDLVVRRASTTAYLPLSSTIQPIISGQPQAA"
     gene            complement(1120..1431)
                     /locus_tag="PK710_08730"
     CDS             complement(1120..1431)
                     /locus_tag="PK710_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09841.1"
                     /translation="MSHEQAWDTLEEAIDKLNAALIARSKGENVPRDVWNAAEEAQTA
                     KEVWVASVMTKDLLLASSVEYSIEGFPDMLGELDAATLWLICRDLWAGNTINAKVRAM
                     E"
     gene            complement(1528..1710)
                     /locus_tag="PK710_08735"
     CDS             complement(1528..1710)
                     /locus_tag="PK710_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09842.1"
                     /translation="MAVVAALRLSWFAIRPDLFTLSARQLREIIVEHRGFLLLGAIAV
                     VWVAVQSVAAALHDVL"
     gene            2153..3034
                     /locus_tag="PK710_08740"
     CDS             2153..3034
                     /locus_tag="PK710_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09843.1"
                     /translation="MGHDLVASREFAIRFQPTGGGRFVPLDGYVAALAELRDGFAGSR
                     HALAPSIAERVRRPTSELRTMLELSVGPAEPGSLIVPVAVTSPMGQLDIEWTKIATLF
                     WRMTANSLWRAARGLAVVGLTAASAQSFAKAAAMGGNRGFVQVELVERRDASKPWRTN
                     VDLTAIERGLRQYVSKKSEPMESTEQLVGRVVALDWDRPSLTLDAPGRKLTVRMPAKL
                     RKTAQTLWGKEVAVTAKAKLTLDGELRDPIALQIEEAVQVSDFVDDFDKSFGRFKDAF
                     ATSEVQDYLRDLRGESS"
     gene            3031..3540
                     /locus_tag="PK710_08745"
     CDS             3031..3540
                     /locus_tag="PK710_08745"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOT09844.1"
                     /translation="MMRALYDTNVVSYWMAGQQEFHTPLRKLQHDLRSLKAMLYVSAV
                     TIQELAVVGQMTGTWPEMHDFIRERFNTVPFTSLCAEKAAALQVAVGKPSKGTKAERR
                     EAKDLWFRDAAIVGTAIAEGFEMVVTTEKWFMHYTNHFEGEIRVVKAVAVVGGASSSE
                     ASRPAKLEK"
     gene            3981..5456
                     /locus_tag="PK710_08750"
     CDS             3981..5456
                     /locus_tag="PK710_08750"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HOT09845.1"
                     /translation="MNHRTASSIIVGCLFAWGAIASGSTWLGCSSDSHEDDRGGTWPL
                     QDGSVEGAQPDAHQTDAATESADTVVVEPTEIDTLFANPGMGWQTFHHVATEDDSLEG
                     IPSGSAYYRYRWLRLQPTQETIDLEEIQNTLDKARAAGQTLMIRVMTAGSDKEYSPDW
                     LAAAGCKIWEYKRDGPTIHAPDLDDPVCWQWHENLLRAIATRFASEPDLQMDIGSVGL
                     WGEWHFSSTTPEIPMPSLETRKKVVDLYRELFPDLPQQALIGDVDALAYATSQGSGWR
                     ADCLGDYGFYSPTWNHMDDMYRQHVEQANATLAWQNGPVAWETCYDMSRWVQEGYDVH
                     RIFQYAVDMHGSFVNNKSAPLPEGSQIRQEIETLLRKLGYRYVLRRLEHPRRVSGTLP
                     VSMDWDNVGVAPAYGPYSLAVRLTSQAGDSVVLSASSKPKAWLPGLTHVQEDLVLPAS
                     MSAGTYQLAVGIIDKTGRAVVKLAIAGEQSGWYPLSEVVVP"
     gene            complement(5752..7608)
                     /locus_tag="PK710_08755"
     CDS             complement(5752..7608)
                     /locus_tag="PK710_08755"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOT09846.1"
                     /translation="MPAARLLWSRPALLCAVIGISCAKDEPPGPLADASEDGCDVVAF
                     EPEISAPPVFTPRWAFEPWISQGVSDEQGIRALLSGFAERQLPAGVVVIDSPWETHYN
                     TFVPSDRRYPKLGELVAHLHAQKVRVVLWMTQMVNRWYHDEKGGDWVVGPSPNFEFAK
                     TCGFFVDEGQTYSWWKGGGAAVDFFHPQARAWWHEQQNALLDLGINGWKLDVGDAFVR
                     SDPVQTAQGQISHQEYSEAYYRDFFAYGAHRKGGLNEFVTLARAWDQPYGRSARFHAR
                     PEHAPVAYVGEKRRDWRGLSDALEHVFRSSRAGYAVVGTDIGGGLEFDPGDPLGDPIP
                     FDRAVFERWLAVGALMPWMHWRGSRDMLPWAMPGCEGDCAQKMLDQHRFWLTFHHELV
                     PFFYSLAHQARAGGAAVLTAHGEPSAWLGDYRFSLGEALLVAPILDDTAIRDIVLPEG
                     QDYYDFWHPEDDDIAGGTILRSYHSPPGQIPLFVRQGAIIPLQVSDEVTGLGTTASRD
                     AWTVLVYPSAKTTSFDLQDTDDVVTTFHAIAGKDTVEVTFSRAIRAVVLQVRMTRRPG
                     QVKLGDAAVPERETRDSLDVAASGWWMDTARQAVWVIVPPSPSPQHIRFLWP"
     gene            7815..8030
                     /locus_tag="PK710_08760"
     CDS             7815..8030
                     /locus_tag="PK710_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09847.1"
                     /translation="MKPMNDCPIQEDSGMPCTAAVVPFTLQGMSAGICSRCGEIVAQS
                     DRRATKKLAKTTTLISLRRASQRHDDT"
     gene            complement(8110..9246)
                     /locus_tag="PK710_08765"
     CDS             complement(8110..9246)
                     /locus_tag="PK710_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09848.1"
                     /translation="MGVSRIYLSPGLFGFSTLGSYHYFCHVEAGLRRRYQAVGKGVQV
                     HIIDVHPTASIRRRGRKLVQVIDETYDGHGAIHLVGHSTGGLDARLVASPTMNVSGAL
                     TTPRWAGAIRSVTTMNTPHFGTPLASFFATLSGQRLLYALSALTVAVLKLGRPPLALT
                     SSLVAAFSGIEKAVGVQLRLIDTLTDKLVRVLDSATADETAQFLRLIREDQGAVIQLS
                     PESMDLFQAGVENSDRVYYQSVASYAPSPGVVSWAKYVVRPWAALSLPVFAMLNRLSG
                     LEHARYPCKPPGTEADDKLRAYFTEPLPDLANDGVVPIRSQVWGDLVWAGRGDHLDVV
                     GHFSDDRDSKDHVDWLSSLSGFDAARFDAMLDGIVEGLLQSENG"
     gene            complement(9330..9917)
                     /locus_tag="PK710_08770"
     CDS             complement(9330..9917)
                     /locus_tag="PK710_08770"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOT09849.1"
                     /translation="MAFVTNCEYAYSMDSGRLVTMVRRGMWLGCSLMVWIAVGCATTA
                     PPPASFPSPLLGKELPSVRGNTVSGETMKPQDLQGRPVVIKFFAKYCEPCKRTLPSAQ
                     RSHVRRGDVLFVGISEDEMVSDAQQLVRAYGLTFPVIHDRDNVLSGRFRVSDLPVTFV
                     VDRCGVVRYVLGPDHEAQVLDQVLDSMSTCGMAGP"
     gene            10042..>11579
                     /locus_tag="PK710_08775"
     CDS             10042..>11579
                     /locus_tag="PK710_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT09850.1"
                     /translation="MGLQRTMPSVTLSLRLLLAFGCVAVLSTSLLGALVRFEWRRTEN
                     ERFDAQVQGAKDGISLEIRAEHDAVQDILRPLCKHDAFVDRTLVDLEAGRLDGGRRLA
                     LAQLVPEEMKALRLDELVMVTGTGEVLGAGHDPSLLGTRRNDFASILSSKNPTGRVRP
                     PSLYDGKPVSAALTGTCAAHAGNRTVGLLGARHIQPVLERLSKAYGVRLALEGTPDAT
                     VLPNEIAQQIVIDEAAGLRVIVAKSRLDLDRHLETLDHWILMTGGVTLAVAILLAFVL
                     ARSLAKPLAALSHEVGEVVAGEPRPVTVRGTPEMRRLAHAFNKTLHDLAALRQRHAAL
                     ERIAAWREVARRVAHEIKNPLTPIRSSVETLRRLKNRDDPTFDEFFDEATKGILDDVH
                     RIATIASDFAKFARLPAPRPAPLDLEDLVRTVVSMHSVQGMTIELVLTPCPIVRADRD
                     QLGQVLTNLIQNAVDAAQSVTTTPHVWVILEPEGNEYIRLRVKDNGPGVAQDLIPKLF
                     EPYVT"
BASE COUNT         2555 a         3542 c         3295 g         2187 t
ORIGIN      
        1 tcgactcgag gtcagcgaca gacagctcga tgcgttgcgc gcgcgagcct ccgacatcga
       61 agcggtggcc actggtgccg tgcagcggac cgaagaatat gtgttgcaag ctttgcaatc
      121 cgactccacc ctggcgtttc gcttctggtc gcgccgagcc ttggatgcca ttgggggtat
      181 tgcttgggct gaagtcaggg ccgtatgtac ccatttgcgg cgtatgatag gggcatcgtt
      241 tcgactcgat ttcaaacgtc gcgagtgttt tctggccgag ctgatggctg cggctgtgcc
      301 ggtggctggg tgattgggca gcgcggtggc cgccgcactt gcgcacagcc atggtcattg
      361 ccggttttac gaaaatgggt acggcgattg atctgtggcc gtggcgtcag gccgcctgcg
      421 gctgtccgga gattattggt tggatggtgc ttgacaaggg caggtaggca gtcgtgctgg
      481 ccctgcggac caccagatcc ttgccgccgt cacgcgtttg cttcgtttgg taaacgtccc
      541 agccttccgc ccgaaagact tcggcgacca agtcctcgaa ctggcgcgga ctgacgacat
      601 cttccaaaca caacccgcga gcaagcaagt agttgtacag caggccaggt gccacaccgt
      661 ccagtcgaac gcgctgcttc agttggacag tcaacccggc gcgctcctcg atgaggtttc
      721 ccagagcgca gttgacaccc accggaaacg tccacacagc gagccccttc cttgcggggt
      781 gtgcgtgagc gactttctcc aactgctgct gcaccctgta ggattcgtca gcgcactgtc
      841 ggtgcttcgc atggccctct tcgtcatgga tgctcggata gaatgtgtag gctcaggcca
      901 agatgcagcg gccctcgaga gtcaattccc acagtcggcg atggaatgcc tcgctgttcg
      961 gtcttgtctc aagggtcacc gacccatcat tgctcgacgg gatgcattcc gatcagcaga
     1021 tcgaagcgct cctcttggga cggggggacc gcaacggcta cggtcagctc gggatgtggg
     1081 gacttggaaa ccatcgaaca tcatgatctg aagtcgaact tactccatag cgcgcacctt
     1141 ggcgttgatc gtgttccccg cccacaagtc ccgacaaatc agccaaagag tggccgcatc
     1201 cagctcgcca agcatgtccg ggaacccttc gatggagtac tcgaccgatg atgccagcaa
     1261 cagatccttg gtcatcacgc tcgcgaccca cacctctttt gccgtctgag cctcttcggc
     1321 tgcgttccag acatcgcgcg gaacgttctc gcccttcgaa cgcgcgatga gcgccgcgtt
     1381 cagcttgtcg atcgcttctt caagagtatc ccatgcttgt tcgtgactca tgggaaaaga
     1441 ctacacgaag cgagactcga tgggcgctac cgacaccgat cgcacgttcg acaaaagacg
     1501 cggggaggta cgtccgagct gctcacctca caatacgtca tgtagcgccg ccgcgaccga
     1561 ctgaaccgcc acccagacaa ccgcgatggc accgagcaac agaaagcccc ggtgctcaac
     1621 gatgatttct cgaagttgcc gggcggagag agtgaaaagg tccggtcgga ttgcgaacca
     1681 agagagtcgg agggccgcga cgacagccat cacgctcaag aagacgcgaa caccagcacc
     1741 aaggtctggg cactgcccat ttgcaacggc aatcaacgcg tagcaggtgg atcccgcaac
     1801 acagcaaact atgggagacg gacaccttga aaggaacgaa cgcatagccg gcctcacgag
     1861 gaccttcaga gcatgcagct ctcgggtctg tgcgagtcgg gtgttagcct gcgagctggc
     1921 ctgtccgacg gtttggttca ggtgccggtg gtccgtcttg tctcacaatt ttcgatcgcg
     1981 accggctgcg gtgcttgcgc acggcttcgt gttgatgggt tacgcccgag acgttccaaa
     2041 acaaagttgg atcgccactg tccaaccgtc atggcgaacg aggtgatcgt ggggggcgct
     2101 cgacctacac tcagagttgc ccaccgatct ctcttgctct acgctctgaa acgtgggcca
     2161 cgatcttgtc gcatcccgtg aatttgccat ccggttccaa ccaaccggag gggggcgctt
     2221 cgttccgctc gacggatacg tagccgcgct cgcggaactg cgggacggct tcgcggggtc
     2281 acgtcatgcg ctcgcgccga gcatcgccga gcgtgtacgt cgcccgacat ccgagctgcg
     2341 cacgatgctt gagctttccg tggggcctgc ggagccaggt agcctcatcg tgcccgtcgc
     2401 cgtcacgtcg ccgatgggtc agctcgacat cgagtggacg aagattgcga cgctattctg
     2461 gcggatgacg gccaactcgc tctggcgggc agcgcggggt cttgccgtcg tcggactcac
     2521 ggcagcgtcc gcgcagagct tcgccaaggc ggctgcaatg ggtgggaatc gaggcttcgt
     2581 tcaggtcgag ctggtagaac gacgcgacgc cagcaagcct tggcgtacca acgtcgacct
     2641 caccgcgatc gaacgcggac ttcggcagta cgtcagcaag aagtccgagc cgatggaatc
     2701 gaccgagcaa ctcgtcggcc gtgtcgtcgc gctcgattgg gatcgacctt cgctcacact
     2761 cgacgctccc ggtcgaaagc tcacggtacg gatgcccgcg aagctgagaa agaccgcgca
     2821 gacgctttgg ggcaaggagg ttgcggtcac tgcgaaggcg aagctcacgc tggatggcga
     2881 gcttcgggat cccatcgctc tgcagatcga ggaagcggtt caggtctctg acttcgtcga
     2941 cgacttcgac aagtccttcg gccgtttcaa ggacgcgttt gcgacgtcgg aggtccagga
     3001 ctacttgagg gaccttcggg gtgagtcgtc atgatgcgcg cgctgtatga caccaatgtg
     3061 gtgtcctatt ggatggcagg gcagcaggag ttccacacgc cgttgcgtaa gctgcagcac
     3121 gatctgcgta gtctgaaggc gatgctttac gtttccgccg tgacgatcca agaactcgct
     3181 gtcgtcgggc aaatgactgg aacctggccg gaaatgcacg acttcatccg cgagcggttc
     3241 aacaccgtgc ccttcacatc tctttgcgct gagaaggctg ccgctctcca agtcgcagta
     3301 gggaaaccat cgaaggggac aaaggcggaa cgccgcgaag ccaaggacct gtggttccga
     3361 gacgcggcaa tcgtcgggac cgccatcgcc gaagggttcg agatggtcgt cacgacggag
     3421 aagtggttca tgcactacac gaaccacttc gagggtgaga tccgagtcgt caaagccgtg
     3481 gccgtcgtgg gcggcgcgtc ctcgagcgag gcttcgcgac ccgcaaagtt agaaaagtag
     3541 ggctggcccc gccgtggccc cgccctgatc gcaccgctcc ccgggggtcc gcacccaatg
     3601 catgccgttc gcgacatctg gcgccccctg gcgacccctt cggggattag gttccagcga
     3661 cgagaggcgt gaggattcaa gtccctcctc gtgcactacg ttgattggaa gggggttgga
     3721 cagagggggt caggcgaatc gaaggggctc cgacgttgtc ccgacgattc ggggttcagc
     3781 ggcccgcagg gttttgtcct ccagcagcac aagccggagg cggtgggcga gcttggcaaa
     3841 gctcagggga ggcgaggcac cgaagatatc gaaatcgctc gacaaaacgc ggggagcgac
     3901 caggtgcagc gtgatcgttg ggcaacgcgc acgagattga tggatcgtgg tggacctcgg
     3961 gatggcgtat agtggtgcgg atgaatcacc gaacggcttc gagcatcatc gtgggctgcc
     4021 tttttgcgtg gggtgccatc gcgagcggta gtacctggct tggatgtagc agtgattcgc
     4081 acgaagacga tcggggcggg acttggccct tgcaggacgg gtcggtggag ggcgcacagc
     4141 cagatgcgca tcaaaccgac gccgccacgg agagcgcaga cacggtggtg gtggagccca
     4201 cggagatcga cacattgttt gcaaatcctg ggatgggttg gcaaacgttc catcacgtag
     4261 ccacagaaga cgattcgttg gaaggcatcc caagcggatc ggcgtattac cgttacagat
     4321 ggcttcgact gcaaccgacc caagaaacga tcgatctgga ggagattcag aacacgctcg
     4381 acaaagcgag ggcagcgggg cagaccctaa tgatacgggt gatgacggcg ggatcggata
     4441 aggaatactc gcccgattgg cttgctgcag cagggtgcaa aatctgggag tacaaacggg
     4501 atggtcctac gatccatgcg ccggacctcg acgacccggt gtgttggcag tggcatgaga
     4561 acttgcttcg agccattgcc acgaggtttg cctcggagcc tgacttgcag atggacatcg
     4621 gttcggtagg actgtggggc gagtggcact tttcgtccac gacgcccgaa atcccgatgc
     4681 cgtcgttgga aacccgcaaa aaggtggtcg atctgtaccg ggagctgttc ccggaccttc
     4741 cccagcaggc gttgataggg gatgtggacg cgctcgcata cgcgacaagc caagggtctg
     4801 gatggcgagc ggattgtctg ggggactacg ggttctactc acccacctgg aaccatatgg
     4861 atgatatgta ccgtcagcac gtggaacagg ccaacgcgac actggcatgg cagaatggcc
     4921 ctgtggcgtg ggagacctgt tacgacatga gtcgatgggt ccaggaggga tacgatgtac
     4981 atcggatctt ccagtatgcc gtggatatgc acggatcgtt tgtgaacaac aagtccgcgc
     5041 cactgccaga gggcagccag attcggcagg agatagagac gctgcttcgc aagctcggat
     5101 accgatatgt gcttcgaagg ttggaacatc cgcgacgagt cagcggcacg ctgcccgtga
     5161 gcatggactg ggacaacgtt ggggtagctc ccgcctacgg accttactct ctggccgtgc
     5221 ggcttacttc gcaagcaggc gattcagtgg tgttgagtgc ctcttccaag cccaaagcat
     5281 ggcttccggg attgacgcat gtgcaggaag accttgtcct tcccgcgtcg atgtcagccg
     5341 gaacgtatca gctagcggtt gggatcatcg acaagacggg gcgcgcagtc gtgaaactgg
     5401 cgattgcggg agagcaatca ggatggtacc cgctcagtga ggtagtcgta ccctagtcct
     5461 cgtcatacac ctaccctccg aaaaatccct ccaaggaccg gatacgtagt ccggtaagga
     5521 gagaggttgg aaggacgcca cggtacccac aaaaagccat acaacctctc ccaggaatat
     5581 ttcgtgttat tattcgttag ttacaacact gccgacaaaa tgacggctaa gcgtttgcgc
     5641 gcgaaaaaaa cgcaagcgct gcccggaatc cacgcgttca accccaaatc ccccggcaaa
     5701 ctccagccgc cgcttggcaa gggatgggtt cgcgcttctg cccgaacgct ctcagggcca
     5761 cagaaagcga atatgctggg gcgaaggcga cgggggcact atgacccaaa ccgcttgtcg
     5821 cgcggtatcc atccaccatc ctgatgcagc aacatctaat gaatcccgcg tttctctctc
     5881 tggaactgcc gcatcgccaa gcttcacctg ccccggtcta cgggtcattc gcacctgaag
     5941 cactacggcg cgaatcgctc gagaaaaggt cacttccacc gtatccttcc ccgcgattgc
     6001 atgaaacgtt gtgacaacat catctgtatc ctgaaggtca aagctcgttg tcttggccga
     6061 gggatagacc agaaccgtcc acgcatcccg gctggccgtc gttcccaagc cggtcacctc
     6121 atcgctcacc tgaaggggta tgatcgctcc ctggcgcacg aataggggaa tctggcctgg
     6181 gggcgagtgg taggagcgaa ggatggttcc ccccgcgata tcatcatctt ccggatgcca
     6241 gaaatcataa tagtcctgtc cttctggcaa aacgatatcg cgaatcgctg tgtcatcgag
     6301 gatgggagcc accagcaagg cttccccaag cgagaatcgg tagtctccca gccaagcgga
     6361 aggctcaccg tgggccgtga gcaccgcagc acctcccgcg cgggcttgat gggccaagct
     6421 atagaaaaac ggcaccaatt catggtggaa cgtcagccag aaccgatgct ggtccagcat
     6481 tttttgcgca caatcccctt cacagcccgg catggcccac ggaagcatgt cgcggctgcc
     6541 tcgccaatgc atccacggca tcaacgcccc caccgcaagc catcgctcga acaccgcccg
     6601 atcgaagggg atgggatcgc ccaacggatc gccaggatcg aattccagcc cgcccccaat
     6661 atccgttccc accaccgcat atccagctcg ggaagaccga aacacatgct caagggcatc
     6721 actcaaacca cgccaatcac gacgcttttc cccgacatac gccacggggg cgtgttcggg
     6781 acgagcatga aagcgagccg aacgaccgta tggttggtcc caggcccggg ccaaggtgac
     6841 gaattcgttc aatccgcctt tgcgatgggc cccataagca aaaaaatccc gataataggc
     6901 ttctgaatac tcttgatggg atatctgccc ctgggcggtc tgcaccggat ccgaacgaac
     6961 gaaagcgtcg cccacatcga gtttccaccc gttgatcccc aggtcgagca acgcgttttg
     7021 ctgctcatgc caccacgccc tggcttgtgg atgaaagaag tccacagccg ccccgccccc
     7081 tttccaccac gaataggttt gcccctcgtc gacaaaaaaa ccgcatgtct ttgcgaattc
     7141 gaagttcggc gacggcccga ccacccaatc gcctcccttc tcatcgtgat accagcgatt
     7201 gaccatctgg gtcatccaga gcacgactcg caccttttgc gcgtgcaagt gggccaccaa
     7261 ttccccgagc ttcggatagc gtcggtcact cggaacgaac gtattatagt gcgtttccca
     7321 cggactatcg atcacgacta cccccgcggg cagttgtctt tccgcaaagc ccgacagaag
     7381 ggcgcgaatc ccctgttcat ccgaaacccc ttgcgaaatc cagggttcga aggcccatcg
     7441 cggcgtaaac acaggcggtg cggagatctc gggctcgaag gcaacgacat cgcaaccgtc
     7501 ttctgaagca tcggccaaag gccccggtgg ctcgtctttg gcgcacgata tcccgataac
     7561 ggcgcataaa agagccggtc ttgaccatag caagcgcgca gcgggcatgc gggatagcct
     7621 agagcacttt gccccaagag caaacggatg ttcgaattgg cagcataatc cttgacggcc
     7681 acgatgaacc ggatggccgc ccctttcgac ctcccccctg gaaccatcct ccctcgggct
     7741 tgccaccctg cacgcgggcc ctacgaatct tcataccccc accatggtgg gcgtatccgc
     7801 aaggaggcag aaccatgaaa cccatgaatg actgccctat tcaagaggac agtggaatgc
     7861 catgcacggc agccgtcgtt ccgttcacac tgcaaggaat gtccgcggga atatgtagcc
     7921 gatgcggaga gatcgttgcc caatccgacc gcagagcaac gaaaaaacta gcaaaaacaa
     7981 caactttaat ctctctgcgt cgcgccagcc aacgccacga cgacacctaa cgttgtggta
     8041 tcatgtcttc cggatccggg cgaaaaagca agctaagaat catggtatcg tgacggtttt
     8101 cgggaaaact catccgtttt ccgattggag aagtccttca acgataccat cgagcatggc
     8161 atcgaaacgc gcggcatcga agcccgaaag ggaggaaagc caatccacat ggtccttgga
     8221 atcccggtca tcgctgaaat ggccgaccac atccaaatgg tcgcctcgtc ccgcccacac
     8281 caggtcgccc caaacctgag agcgaatggg tacgacccca tcattggcga gatcgggaag
     8341 gggttcggtg aaataagccc gcaatttgtc atcggcttcg gtgcccgggg gcttgcacgg
     8401 ataccgggca tgctcgaggc cggaaagacg attcagcatg gcaaacacgg gcaaggaaag
     8461 ggcagcccat ggacgtacca catattttgc ccaggaaacc acacccggac tgggcgcata
     8521 ggaagccacc gattggtaat acacgcgatc actattttcg acccccgcct ggaataaatc
     8581 catcgattcg ggagaaagct gaatgaccgc gccctgatct tcccgaatca agcgcaaaaa
     8641 ctgcgccgtc tcatccgctg tcgcactgtc caatacccga acgagcttgt cggtcagcgt
     8701 atcgatgagc cgaagctgca cccccaccgc cttttcgatc cccgaaaacg cggccaccag
     8761 cgatgacgtc agcgcaagcg gcggacgacc aagcttcaga accgcaacgg taagtgctga
     8821 taacgcatac agcaaacgtt gccccgacaa cgtagcaaaa aaactcgcta gaggcgtccc
     8881 aaaatgcggc gtgttcatcg tcgtcaccga acgaatcgct ccagcccaac gcggcgtcgt
     8941 caacgcccca ctcacgttca tcgtggggga ggccaccagc cgcgcatcca acccccccgt
     9001 cgaatgcccc accaagtgga tcgccccatg cccatcgtac gtctcatcta tcacctgcac
     9061 cagcttgcgt ccccgacgac gaatggaagc ggtgggatga acgtcgatga tatgcacctg
     9121 aacgcctttg cccacggctt gatagcgtcg acggagccct gcctccacat ggcaaaaata
     9181 atggtacgaa cccaacgtcg aaaaaccgaa caacccgggg gataagtaga ttcgcgacac
     9241 acccatgccc aacaatgttc atgagacggc gccgatccgc aaggactaca tgcccatccc
     9301 ttcgaatcat cggacccgga tcgtggcgat cacggccccg ccatcccgca ggtactcatc
     9361 gaatccaaaa cctgatccaa cacctgcgct tcatggtcgg gaccaagcac ataacgaacg
     9421 accccacaac gatccaccac gaacgtcacc ggcaggtccg acacgcgaaa acgacctgat
     9481 aagacattat cacgatcgtg gatgaccgga aatgtcagcc cgtaggcacg aacgagctgc
     9541 tgcgcatcgg aaaccatctc atcctcggaa atcccaacga aaagcacatc cccccgacga
     9601 acatggctgc gttgcgccga gggaagcgtg cgcttgcatg gctcgcaata tttggcgaaa
     9661 aacttgatga cgaccgggcg gccttgcaga tcctgcggtt tcatcgtctc gccactcacc
     9721 gtatttccac gtaccgaagg tagttccttc cccagcaacg ggcttggaaa agaggcgggg
     9781 ggaggtgccg tcgttgcgca tcccacggcg atccacacca tcaacgagca tcccaaccac
     9841 atcccgcgcc gtaccatcgt gaccagccgt cctgaatcca tcgaataggc atactcgcag
     9901 ttcgtgacaa acgccacgtc ggcttcgctc ccccctgccc gaacgattct tcaccctata
     9961 tgcacacgtt gcttcatgaa aaacccgtgg cggagcgttt cccccaaaga caactttcac
    10021 cccttgtcga ggtggtagcc catggggttg cagcgtacca tgccctccgt gactctctcc
    10081 ctacgtttgc tccttgcatt tggttgcgtg gcggtcttgt cgacctcctt gctcggagcg
    10141 ctcgtgcgct tcgaatggcg acgtaccgaa aatgaacgtt tcgatgccca ggtgcagggg
    10201 gcaaaagatg gtatttctct cgaaatacgc gcggaacacg atgccgtgca agacatcctt
    10261 cgacccttgt gcaaacacga cgcgttcgtg gaccgcacac ttgtcgacct cgaggccgga
    10321 cgattggatg ggggacgccg cctcgccctt gcccaactcg tgcccgaaga aatgaaagcg
    10381 ttgcgcctcg atgaactggt catggtcacc ggcacgggtg aagtgctcgg tgccgggcat
    10441 gatccttctc tgctcggaac gcgtcgtaac gacttcgcaa gcattctgtc atcgaaaaac
    10501 cccaccggta gggttcgtcc gccatcttta tacgatggca aacccgtttc cgccgctctc
    10561 acgggaacgt gtgcagcgca cgcgggaaat cggacggtcg gattgctcgg cgcaagacat
    10621 atccaacccg tgctggaaag actgagcaaa gcctatggag tacgcctcgc cctcgaaggc
    10681 acccccgacg ccaccgtgtt gcccaacgaa atcgcacaac aaatcgtgat cgatgaagcc
    10741 gcggggttac gcgtcatcgt cgcaaaatcc cgtctcgacc tcgaccgtca cctcgaaacc
    10801 ctcgaccatt ggatactcat gaccggcggc gtcacccttg ccgtcgccat tttgctcgcc
    10861 ttcgttctcg caagaagcct cgcaaaacct ttggccgcct tgtcccacga agtcggtgaa
    10921 gtcgtcgctg gcgaacccag acccgtcacc gtccggggaa ccccggaaat gcgtcgcctc
    10981 gctcatgcgt tcaacaaaac cttgcatgat ctcgctgcct tacgtcaacg ccacgccgcc
    11041 ctcgaacgaa tcgcggcatg gcgcgaagtg gcgcgtcgtg tcgcccacga aatcaaaaat
    11101 cccctcaccc ccatccgatc ctccgtcgaa acgttgcgac ggctcaaaaa ccgcgacgat
    11161 ccgacgttcg atgaattttt cgacgaggcg acgaaaggca ttctcgacga tgtgcatcgc
    11221 attgccacca tcgcttccga ctttgccaaa ttcgctcgac tcccggctcc tcgtcccgcg
    11281 cctttggatc tggaagacct cgttcgtacc gtggtgtcga tgcatagtgt ccaaggaatg
    11341 acgatcgaat tggtgttgac cccgtgtccc atcgtgcggg ccgatcgaga tcaactcggg
    11401 caagtgctga ccaatctcat ccaaaacgcc gtggatgcgg cacaaagcgt gacaacgacc
    11461 cctcacgtat gggtcatcct tgagcccgag ggaaacgaat acatccgtct tcgtgtcaaa
    11521 gacaacggac cgggcgtggc ccaggacttg atcccgaagc tcttcgaacc ctatgtcac
//
LOCUS       DAOSOR010000122        11537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5576_length_11537_cov_3.967166, whole genome shotgun sequence.
ACCESSION   DAOSOR010000122 DAOSOR010000000
VERSION     DAOSOR010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11537
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5576_length_11537_cov_3.967166"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PK710_08780"
     CDS             <1..266
                     /locus_tag="PK710_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09851.1"
                     /translation="GGGLIDRKGDHAQITMSMSGDIACTALVKSAVMSRLGKGWAGLV
                     ADAARRALAGSVRVAVKVSADSRKLTEAKVHHDVGFGCTVRLL"
     gene            complement(279..602)
                     /locus_tag="PK710_08785"
     CDS             complement(279..602)
                     /locus_tag="PK710_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09852.1"
                     /translation="MANRTSYAGPERRIHKVYVTRNTEYHVREGMCVAVKSRQDSALT
                     TDHSAVKMKLEGHVKLGTLLPVAGPPKIGFRMYFAKGEDDVLTSPVIAILRPAKKTVD
                     QYPKD"
     gene            736..1686
                     /locus_tag="PK710_08790"
     CDS             736..1686
                     /locus_tag="PK710_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09853.1"
                     /translation="MRPFACEHCGSDLHLSLPDKAYLCEALPTEHRWDRGQLEQAWLR
                     QASERVEGSPDDFDSVFQLASLCGRLASTSLRAGDSDPARVYLSQVVAVIDAYHQRDP
                     TEERSRAMLMEALVEQATVSEKLRDFGIAASAYERLASLVGAEAEASPKSIEQQRNLS
                     SCYNGAGRMNRAVGNLVAAKQFFEKDLGVIDKLCDLYPEHPDLPIDRAVAHFNLFLVS
                     TSREEELLHLDMTLEILDRLPADGAQQVEQLAQRARAERKRLMDTDDTASERLSKVTA
                     DMVRSKEQDASKRPVDDRAWLIAQVDGVLRARRKRLHVLR"
     gene            1732..2157
                     /locus_tag="PK710_08795"
     CDS             1732..2157
                     /locus_tag="PK710_08795"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HOT09854.1"
                     /translation="MSEQCSISLDVVKEALQALGWKYEQGDEGIGVTFQAPGVPSIHV
                     EFKVAGARLLATSAIDVPIPRQKWAEVLVDLNQFNATHFRPKAYLSMTSESDVGMLIG
                     EEVHDLAGGTTVELVGAWMGGHANAFAACASTVLGVRLE"
     gene            2438..3883
                     /gene="atpD"
                     /locus_tag="PK710_08800"
     CDS             2438..3883
                     /gene="atpD"
                     /locus_tag="PK710_08800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOT09855.1"
                     /translation="MAQEQTTQNVGKVLQVIGPVVDVAFPPGKLPAIYNALKMNNPSI
                     SDAEDNLILEVAQHLGESTVRTIAMDSTDGLVRNMVVTDTGKPIMMPVGKECLGRILN
                     VVGRPVDEAGPVHAQTFLPIHREPPKFVDQSTKIEIFETGIKVVDLLAPYRKGGKIGL
                     FGGAGVGKTVLIMELINNVAKAHGGVSCFAGVGERTREGNDLFWEMTESKLESGASVI
                     SKAALVYGQMNEPPGARARVALSALTAAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSA
                     LLGRIPSAVGYQPTLATEMGELQERITSTTKGSITSVQAIYVPADDLTDPAPATAFAH
                     LDATTVLARSIAELGIYPAVDPLDSTSTMLDPRVVGDRHYRVAMDVQQILQKYKDLQD
                     IIAILGMDELSEDDRLVVSRARKIQRFLSQPFFVAAQFTGFAGRFVSLKDTIDGFEQI
                     VSGKHDDIPEQAFLYCGGIDEVLAKAAKMKA"
     gene            3888..4400
                     /gene="atpC"
                     /locus_tag="PK710_08805"
     CDS             3888..4400
                     /gene="atpC"
                     /locus_tag="PK710_08805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOT09856.1"
                     /translation="MANLKALPRPGGKIRLEVVTPEGLALNADVDEFTSPSVQGQFGV
                     LPGHLPLLAALRTGVVSYRQGQQTVVCAVGPGFVEVVDDHAIMLTDRFADKDSVDIVD
                     VRMRLKEIESKLEKWDEPGTPEHMSVIEEELWLAALLELSGDPPPPTMRPTRLPAQKA
                     KPTSEISDES"
     gene            complement(4511..5383)
                     /gene="lgt"
                     /locus_tag="PK710_08810"
     CDS             complement(4511..5383)
                     /gene="lgt"
                     /locus_tag="PK710_08810"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOT09857.1"
                     /translation="MEWNFNPVALDLGFFQIRWYGVAFMAGLLIGATEMSRALQRRGL
                     PKDHANSLTLWVPVGMLLGAHFVHLLFYEPRSFIDNPRRIFEVGLGLASHGGGLGAIL
                     ATYLFTRKHKLDFHQYLDVVLTGAVFVIPAVRIGNFFNSEIYGRVTDVPWGVVFVRRG
                     FTEPRHPSQLYEAAIGVALLALTLFLEHKYRNRLRNGALFYLILTLYFITRFIVEWFK
                     EYQVLDPSFPFTMGQLLSVPFVLFFGYMLFFSKNHNIRKPPESTPVPAAPAPPTASEA
                     SESTDSNEPDDDPT"
     gene            5810..6697
                     /locus_tag="PK710_08815"
     CDS             5810..6697
                     /locus_tag="PK710_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HOT09858.1"
                     /translation="MDRIRVVGWAMGFGMGLVMLACNGGPQDVENVSQAAEPLGSEMK
                     PELRKAPGRPGVYGLALEADKNGDGKVTEAEFLGAVENRFQNLDSDGDGFLEGTECLG
                     RMQDMPGSSRGMRRGMPMMRRMDKDGDGKISKSEYVQGHKELFETMAEGSGVLSIDKI
                     WQEGNGRAGFGHGMGPRMRGRDRFGQLDTNGDDKVTKAEAEAGHALRFQQMDKNQDGV
                     IDADEMKAYREAKMPGFGRHTGRWTRMDTDGDGKVTKAEFDRWHTEMFQRWDENGDGE
                     VSLEEFGAHRPMAGPGRAR"
     gene            6754..7350
                     /locus_tag="PK710_08820"
     CDS             6754..7350
                     /locus_tag="PK710_08820"
                     /inference="COORDINATES: protein motif:HMM:NF004113.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT09859.1"
                     /translation="MDPHSHEKNYPTQAPEDVELVRRVAAGDRIAGEVLVERHLRLIV
                     GFAFRLLGDMAEAEDVAQETFLRLWKHADRWEPRAKLSTWLHHVARNLCIDRLRLRRG
                     REGEVMELPDLRPGVVTLLESRQRDDAVQRALLQLAERQRSAIVLVYYQGLSNREAAQ
                     VLGVEVDALESLLARGRRTLRKLLMGVEGETAKREIVS"
     gene            7347..7823
                     /locus_tag="PK710_08825"
     CDS             7347..7823
                     /locus_tag="PK710_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09860.1"
                     /translation="MKRERAQTKQVKLDRFAELVEAYGANPKRWPEDERDAALMWLES
                     SEDARRLVEEANHLDRWLESAGSITPSPALKGRIMAQMPRVSEVPLSPWMRWVERIWP
                     FGPTWQPVAALVAAAMLGVTFGVVMPESDESSSGGMVVAEVLLDMGDLADDWNEGP"
     gene            7820..8350
                     /locus_tag="PK710_08830"
     CDS             7820..8350
                     /locus_tag="PK710_08830"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOT09861.1"
                     /translation="MNMRNAKTWAVIAVLSLAANLFLGGLLVGRSFRSDKPLPPPSVI
                     GPIALLRSSQDLDPAARQIVENAREKHREEIHRSMMSASRSRRAAIEAVCAKDFDEAK
                     AREAFRAYRDQTVGAHDVMHESLIDAAKQMTAEQRMVLREVLMRSGGRGGWKGRGRGV
                     GAGFRKQVPQEALEEP"
     gene            complement(8355..9668)
                     /locus_tag="PK710_08835"
     CDS             complement(8355..9668)
                     /locus_tag="PK710_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HOT09862.1"
                     /translation="MKKPSVITPPNGKLGVLLPGLGAVATTFVAGCLLARKGLAKPIG
                     SLTQLGTIRLGKRTDNRVPLLKDFLPIATLDQLVFGGWDIFPDNAYESALNAQVLERR
                     HIDPVKEELEQVKAMPGVFYPEYVKRLHGTHIKQGKTKADMVEQLREDIRSFKKNNGC
                     DRLVAIWCGSTEVLVDPSPAHASIEAFEKALRENDPAISNSMMYAWACLKEHVPFANG
                     APNLTVDIPAMTLLARDMAVSIAGKDFKTGQTLMKTILAPGLKARMLGLDGWFSTNIL
                     GNRDGEVLDDPGSFHTKEVSKLGVLDTILQPSVYPELYGNIHHKVRIEYYPPRGDSKE
                     GWDNLDIFGWLGYPMQIKVNFLCRDSILAAPIVLDLALLMDLGQRAGFTGIQEWLSFY
                     FKSPMTAPGLYPEHDLFIQAKKLKNTMRWMMGEDLVTHLGNEYYG"
     gene            9768..10874
                     /locus_tag="PK710_08840"
     CDS             9768..10874
                     /locus_tag="PK710_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOT09863.1"
                     /translation="MRRRRAEAGAYAWLFRLWRAVLLWVWIRSCGMEIVIPPLACWRE
                     TCKPRASMSILDGYKASLKPLEVEEPIDVYVHRPLGYLVALLTFRTPISPDLITLASI
                     VAGIGAGVCIVWRFPYHLPVAGALVFLSAVLDCADGQLARMRKTSSAFGRMIDGVADL
                     IVMLSVVPAALVQVWRLYDNDPWLAGTVVGISIFAVVTSSFHTGMYDHYKNVYLHLAH
                     PSYRDGEDYETARERWEKGASGQPFWKRLSWQVYFFYIKSQAGYVAGFDPYTSARFAL
                     FPAYDPQRAAVYREQNLRVLRVWRSLFGFGSLVFGTAMFVALGRPDVYLLYRLLILNG
                     IFYLYLRPAQRRASRQAFEQMGLRMPDQKWSDGT"
     gene            10871..>11537
                     /locus_tag="PK710_08845"
     CDS             10871..>11537
                     /locus_tag="PK710_08845"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOT09864.1"
                     /translation="MTQKRGCAPVAVVLVAGIGERLHPLTLHRPKALVQVGGESMLAR
                     SVRLLLAHGVEQIVLATGFKEDAVREAMQGVPVPVSYQLNPRYRETQNAVSLLACEQA
                     VAGRAFYKLDGDLLFRAEVLERLDEVDSGLVVAMDRSVELGQEEMKIRLKQGGTSIAA
                     FGKGLEPSSCHGESIGIERVNEVAVSLIFHGLRQAVEAGETHLYYEDVYGRLVDHGLE
                     ARGV"
BASE COUNT         2410 a         2535 c         3938 g         2654 t
ORIGIN      
        1 agggaggagg tctgattgat cgaaagggag atcatgcgca gatcacgatg agcatgtccg
       61 gggatattgc gtgtacggcg ctggtgaagt cggcggtcat gtcgcgattg ggaaagggtt
      121 gggcagggct tgtggcggac gcggctcgtc gggctctggc cggttcggtg cgagtggcgg
      181 tgaaggtgag tgcggactca cgaaagctta cggaagcgaa ggtgcatcac gacgttgggt
      241 ttggatgcac cgtgcggttg ttgtgaaggg atggggattt agtctttggg atattgatcg
      301 acggtttttt ttgcaggccg aaggatggcg atgacggggc ttgtgagaac gtcgtcctct
      361 cctttcgcga aatacatgcg aaagccgatt ttggggggac cggccacagg cagaagggtg
      421 ccgagtttga catggccttc cagcttcatt tttacggcgc tgtggtccgt ggttagcgct
      481 gagtcttgac gtgacttgac ggccacgcac atgccttcgc gcacgtggta ttcggtattt
      541 cgggtgacat agactttgtg gatgcggcgt tcggggccag cgtatgaggt gcgattcgcc
      601 atgggttagg gtctccaaaa cggcagcatg cccaagcagg agggggacgg ccaagaaatg
      661 ggctgaaagt cgacaattgc gggggttgaa tcggttctca ttgaccatcg cgcacgccgt
      721 cgatacagac gagcgatgcg accgtttgcg tgtgaacatt gtggatctga tcttcaccta
      781 tcgttaccgg acaaggcgta tttatgtgaa gctttaccga cggagcatcg gtgggatcgt
      841 gggcaactgg agcaggcgtg gttgaggcag gcatcggagc gtgtggaggg gtcgcctgac
      901 gatttcgatt ctgtctttca acttgccagt ctttgcggac gactagcctc gacgtcgtta
      961 cgcgctgggg attccgatcc cgcgcgtgtg tatttgagcc aggtggttgc cgtgatcgat
     1021 gcgtatcacc aacgtgatcc gaccgaagag cggtcgcggg cgatgctgat ggaggcattg
     1081 gtcgagcaag ctaccgtgag tgaaaaacta cgtgatttcg ggatcgcggc ctcggcttac
     1141 gaacgtctgg cttccttggt gggtgctgag gcggaagcat cgccgaagag catcgagcag
     1201 cagcgcaatc tgtccagttg ctacaatggc gcggggcgta tgaaccgggc ggtggggaat
     1261 ttggtcgctg cgaagcaatt tttcgagaag gaccttggtg tgatcgacaa gttgtgtgac
     1321 ttgtatcctg agcatcccga cttgcccatc gaccgtgcgg tagctcactt caatctattt
     1381 cttgtgagca cgtcacgaga agaagagctg ttgcatctcg atatgaccct cgagattttg
     1441 gatcggctgc cggccgatgg agcgcaacag gtggagcagc ttgcgcagcg tgcgcgggcg
     1501 gagcgaaagc gattgatgga tacggatgat acggcatcgg agcgtctttc caaagtcact
     1561 gcagatatgg tgcggtccaa ggagcaagac gcctcgaagc ggccggtgga tgatcgagca
     1621 tggcttatcg cgcaagtcga cggtgtgctg cgagcgcgac gaaagcgttt gcatgttttg
     1681 cggtgagaag acatggcggg ctaggcacac ctcgatgaca agggagcaat catgagtgag
     1741 cagtgttcga tttcgttgga tgtcgtgaaa gaggcgttac aagccttggg gtggaagtac
     1801 gaacaagggg atgaggggat cggggtgacg tttcaagcgc cgggtgttcc cagcattcat
     1861 gttgagttca aggtggcggg cgcacgactt ttggccacca gcgccattga tgttccgatt
     1921 ccaaggcaaa agtgggcgga ggtgttggtg gatctgaatc aattcaacgc gacgcatttc
     1981 cgtccgaagg catatttgag catgacctcg gagtcggatg tagggatgtt gattggcgag
     2041 gaagtgcatg atcttgctgg tgggacgacc gtagagttgg ttggggcgtg gatgggaggg
     2101 catgcgaatg cgtttgccgc ttgtgcgtcc accgtgcttg gtgttcgatt ggaatagtaa
     2161 acctagaaat tactttcgac gaaacggggg cgtatgatct gataatcaaa ataacatgtt
     2221 atttcaatag gttatattat attgcccgcg agggtatcag caaccatggg attcgtgatc
     2281 ggttggcaac ccgtcgtctc cgtgttagag gcgcaacgcc gcgtcgcgtt gtgcggcacc
     2341 cctgtgggct atgtccggca ggagatagtc cgcgaagcat ttgagacacg gccggaaaac
     2401 cggtcgcatg cacggaaggg tttggaggct gagattcatg gctcaagaac aaaccacgca
     2461 gaacgtcggc aaggtgttgc aggtcattgg accggttgtt gacgtggcgt ttccgccggg
     2521 taagttgccg gccatctaca atgcgctcaa gatgaacaac ccatccatct cggatgctga
     2581 ggacaacttg attttagaag tggctcagca tctcggggag tccacggtgc ggacgatcgc
     2641 catggattcc acggatggtt tggtccgcaa catggtggtg acggatacgg gcaagccgat
     2701 catgatgccc gtgggcaagg agtgtcttgg gcgcattctc aatgtcgtgg gtcgaccggt
     2761 agacgaagcg ggtccggtcc atgcgcagac gtttttgccc attcaccgtg agcctcccaa
     2821 gttcgtcgac caatcgacga agatcgagat tttcgagacg gggatcaaag tggtcgattt
     2881 gctcgcaccg tatcgcaagg gcggcaagat cggattgttt gggggtgcgg gtgttggcaa
     2941 gacagtgctc atcatggaac tcatcaacaa cgtggcgaag gcccatggtg gtgtttcctg
     3001 cttcgcgggt gtgggagagc gaacccgtga aggtaacgat ttgttctggg aaatgaccga
     3061 atccaagctc gagtctggtg cttcggtcat ttcgaaagcc gcgttggttt acggtcagat
     3121 gaatgagcct ccaggggcgc gtgcgcgtgt cgccttatct gccctgaccg cggcggagta
     3181 cttccgtgat gtggagggcc aagacgttct attgttcatc gacaatatct tccgattcac
     3241 gcaagccggt tcggaagtat ccgctttgct tggccgaatc cccagcgcgg tgggttatca
     3301 gcccacgctc gcgacggaaa tgggggagtt gcaggagcgg attacttcga ccaccaaagg
     3361 gtccatcact tctgtgcagg cgatttacgt gcccgcggat gacttgacgg acccggcccc
     3421 agccacggcc tttgctcatc tcgatgccac gacggtgctt gcgcgaagca ttgcggaact
     3481 tggcatttac cctgccgttg acccgctcga ttccacatcg acgatgctcg accctcgcgt
     3541 ggtgggagat cgtcattacc gggtcgccat ggatgtgcag caaatcttac aaaaatacaa
     3601 agacttgcaa gatatcatcg ccatcttggg catggatgag ttgtccgagg acgatcgtct
     3661 ggtggtctct cgagcgcgta agattcagcg cttcctgagc cagcccttct ttgtagcggc
     3721 tcaatttact ggttttgccg ggcgattcgt ttctctcaag gatacgatcg acggatttga
     3781 gcagattgtt tccggaaagc atgatgacat tcccgaacag gcgtttttgt actgtggtgg
     3841 aatcgatgaa gtcttggcga aggctgccaa aatgaaggcg taagacgatg gcgaatctca
     3901 aagcattgcc tcgaccagga ggcaagatcc ggcttgaagt cgtgacgcca gagggcttgg
     3961 ccctcaacgc ggatgtggac gagtttacat cgccttctgt gcaaggacag ttcggcgtgt
     4021 tgcctggtca cttgccgctg ctggctgctc ttcgaacggg tgttgtgtcg taccgtcagg
     4081 gacaacaaac cgtggtatgt gccgtgggtc ccggcttcgt cgaggtggtc gatgaccacg
     4141 cgattatgct gacggatcgg tttgccgaca aagactcggt cgatattgtc gacgttcgca
     4201 tgcgcctcaa agagatcgaa agcaaactcg agaaatggga tgagcccgga acacccgagc
     4261 acatgagcgt cattgaagaa gagttgtggc ttgcggctct gctcgaactc agcggtgatc
     4321 cgccgcctcc aacgatgcgg ccgacgcgac ttccggcgca aaaggcgaaa ccgacctcgg
     4381 aaatctctga cgaaagctga ccttcgtgtc agcgagtttt ccaatcaccc taccgcgaac
     4441 gatggctcct gtttcgtgct aaggcacgag gctaggtcgc cttgggtgta gtggcgtcat
     4501 cgcctgagtg ttacgtgggg tcgtcgtcag gctcgttcga gtcggtcgat tcagaggctt
     4561 ctgaagcggt gggcggagca ggcgctgcgg gaacgggagt ggattccgga ggtttccgaa
     4621 tgttatggtt tttgctgaaa aatagcatgt agccgaagaa tagaacgaag ggaacgctaa
     4681 gcagttgtcc catcgtgaag gggaaagaag gatcgagaac ctggtattcc ttgaaccatt
     4741 cgacgatgaa tcgagtgatg aaatacaggg tcaaaatcag gtagaatagg gcaccgtttc
     4801 gaagacggtt gcgatatttg tgctcgagga aaagggtaag cgccagcagg gcaacgccaa
     4861 tggcggcttc atacaattgt gatggatggc gcggctcggt gaatcctcgt cgaacgaata
     4921 caacgcccca tggaacgtcg gtgacgcggc cgtagatttc ggagttgaag aagttgccga
     4981 ttcgaaccgc gggaatcacg aacacggctc cggtgagcac gacatcgagg tattggtgga
     5041 agtcgagttt gtgttttcga gtaaacaagt acgtggcgag gatggcaccg agcccgccgc
     5101 catgactggc gagtccaagt ccaacctcga agattcgacg agggttatcg atgaacgaac
     5161 gcggttcata aaataaaagg tgaacgaaat gggcgcccag aagcattccg acgggtaccc
     5221 acagcgtaag ggagttggca tggtctttgg gtaagcctcg tcgttgcagc gcccgtgaca
     5281 tctccgtggc gccgatgagc aggcctgcca tgaaagcgac tccgtaccag cggatttgaa
     5341 agaaaccgag gtcaagagcg actgggttga agttccattc catggatacg tggatccttc
     5401 atttcggaaa tcggaattga gacggtgggg aatagaacat gagggcggga tagtcgattc
     5461 cgaaccgctt cattcgccga cagcacggaa tgacgaccgg aaatggcggg tcggcatttc
     5521 ttactcgtcg acaggttatc agcgtttgac atggacactg gctcgatccg acggatcgga
     5581 gcgtagggtt cgtagatcgc gtgctgccca tggtcaagcg cggggctgat ttcgataagg
     5641 ttgcggaaga gtgatgcggg cggtgagtct gaacattttg gggaggggga gcgacggatc
     5701 gggcgagatg tttcgttcga tcctttgatg cgccactcgg ccatgcccga aagctacgaa
     5761 ggcgggtttg gtggcagtat tacaaagaca acgagaaaag aggccaagga tggatcgaat
     5821 tcgagtggtt ggttgggcga tggggtttgg gatgggattg gtcatgttgg cttgcaacgg
     5881 ggggccgcag gatgtcgaga acgtttcgca ggcggcggag ccgttggggt cggaaatgaa
     5941 accggaattg cgcaaggctc caggcagacc aggggtgtat gggcttgcgc tggaagccga
     6001 taaaaatggt gatgggaagg tgacggaagc tgagttccta ggggcagtcg aaaataggtt
     6061 tcagaacctc gacagtgatg gggacggttt tttggagggg acggagtgtt tgggacgcat
     6121 gcaggatatg ccgggctctt ctcgagggat gaggcgaggg atgccgatga tgcgtcgaat
     6181 ggataaggat ggggacggca agatatcgaa atcggagtat gtgcaagggc ataaggagtt
     6241 gtttgaaacg atggcggaag ggtcgggagt cttgtcgatt gacaagatat ggcaagaggg
     6301 caacggcagg gcagggtttg gacatgggat gggaccgagg atgcggggac gagatcgatt
     6361 tgggcagttg gacaccaatg gggatgacaa ggtgaccaag gcggaggcgg aggctggtca
     6421 tgccttacgt tttcaacaga tggataagaa tcaggatggt gtgatcgatg cggatgagat
     6481 gaaagcgtat cgagaagcaa aaatgcccgg atttggacgt catactgggc gttggacgag
     6541 gatggacacg gatggagatg gaaaggtgac gaaagcggaa ttcgaccgct ggcatactga
     6601 gatgtttcaa cgatgggatg agaatgggga tggggaagtg agtctcgagg agtttggggc
     6661 gcatcgaccg atggcaggac cggggcgtgc gcgttgacac cgagggcgct gtgtcggacg
     6721 gtctgttgat tggctcctta ctgaactgta ggcatggatc ctcattccca cgagaagaac
     6781 tacccaacgc aagcgccaga agatgtggag ttggtgcgtc gggtagccgc tggggaccgg
     6841 atagcgggcg aagtattggt ggagcgccat ttgcggctga ttgtaggttt tgcgtttcgg
     6901 ttgctcgggg acatggcgga agcggaggat gttgcgcagg agacttttct tcggctttgg
     6961 aagcatgccg atcgatggga acccagggcc aagctatcga cgtggttaca tcatgtggcg
     7021 cgcaatctat gtatcgatcg gcttcgtttg cggcgtggga gagaaggcga agtgatggag
     7081 ttgccggact tgcgtccagg agtggtcacg ctgctggaga gtcgacagcg agacgacgcg
     7141 gtgcagcgag cgttgttgca attggccgag cgtcaacgca gtgcgattgt gctggtgtat
     7201 taccaggggt tgagcaatcg agaagccgcg caagtgctag gcgtcgaggt agatgctctc
     7261 gaatcgttgt tggcgcgagg gcgtcgcact ttgcgcaagc tactgatggg ggttgagggc
     7321 gagacggcga agagggagat tgtgtcatga agcgcgaacg ggctcaaaca aagcaggtca
     7381 agctcgatcg gtttgcggaa ttggtggaag cctacggtgc aaatccgaag cgctggcccg
     7441 aagacgagcg agacgcggcg ctcatgtggt tggagagcag tgaagacgcg cggcgcttgg
     7501 tggaggaagc gaatcatctc gatcgatggt tggagtcggc gggtagcatt acgccttctc
     7561 ccgcgttgaa agggcggatc atggcgcaga tgccgagggt atcggaggtg ccattgtcgc
     7621 cttggatgcg ttgggttgag cgtatttggc cgtttgggcc gacgtggcag cctgtggcgg
     7681 ccttggtggc agcagcgatg cttggggtga cgtttggtgt tgtgatgccg gagtcggacg
     7741 aatcgtcttc aggggggatg gtggttgcgg aggtgcttct ggacatgggt gatctggcag
     7801 atgattggaa cgagggacca tgaacatgcg caatgccaag acttgggcgg tgatcgcggt
     7861 attgtcattg gccgcaaatc tgtttcttgg tgggctgctg gtggggcgga gctttcgttc
     7921 tgacaaaccg cttccaccgc cttcggtcat cggtcccatc gctttgcttc gttcttcgca
     7981 ggatttggat cctgctgcca ggcagatcgt tgaaaacgcg cgcgagaagc atcgcgagga
     8041 gattcatcgg tcaatgatga gcgccagccg ttcgcgtcgt gcagccatcg aggctgtttg
     8101 tgccaaggat ttcgacgaag ccaaagcgcg agaggcattc cgggcgtatc gcgatcagac
     8161 cgtgggggct catgatgtga tgcatgagtc gttgattgat gcggcgaagc agatgacagc
     8221 ggaacagcgg atggtgcttc gagaggtatt gatgcgaagc ggaggtcggg gagggtggaa
     8281 aggaaggggg cgcggtgtgg gagcaggctt tcgaaagcaa gtccctcagg aggctttgga
     8341 ggaaccgtag gggatcagcc gtagtattcg ttgccgaggt gggtgacgag gtcttctccc
     8401 atcatccatc gcatggtgtt tttcagtttt ttcgcttgga tgaatagatc atgttcggga
     8461 tagaggccgg gggctgtcat ggggctcttg aagtagaagc tgagccattc ttgaatgccg
     8521 gtgaagcctg cacgttgccc gagatccatg agcaacgcta gatccagtac gatgggtgcc
     8581 gcgaggatcg aatctcgaca caagaagttg accttgatct gcatgggata accgagccat
     8641 ccgaagatat cgaggttgtc ccatccttct ttcgaatctc cgcgcggtgg gtagtactcg
     8701 atgcgaacct tgtggtgaat gttgccataa agttctgggt agaccgacgg ctgaagaatc
     8761 gtgtcgagca ctccgagttt cgaaacctct ttggtatgaa atgaccctgg atcgtcgagg
     8821 acttctccat cgcgattgcc caaaatattg gtggagaacc agccatcgag accgagcatg
     8881 cgggctttga gtccgggggc aaggatggtt ttcatgaggg tttgaccggt tttgaagtct
     8941 tttcccgcaa tggagacagc catatctcgg gcgagtagtg tcatggcggg gatatcgacg
     9001 gtcaggttgg gggcaccgtt ggcgaagggg acatgttctt tgagacaggc ccacgcgtac
     9061 atcatggaat tggagatcgc ggggtcattt tctcgcaaag ctttttcgaa ggcctcgatg
     9121 gaagcgtgtg cggggctagg gtcgacgagc acctcggtgc ttccgcacca aatggcaacg
     9181 agacggtcgc atccgttatt ttttttgaat gaacgaatgt cctcgcggag ttgttccacc
     9241 atatccgctt tggttttccc ttgtttgatg tgggttccat gcaggcgttt gacgtattcg
     9301 ggatagaaaa cgccgggcat ggctttgact tgttcaagtt cttctttgac ggggtcgata
     9361 tgtcgacgtt cgaggacttg cgcgttgagg gcggattcat aagcgttgtc gggaaagatg
     9421 tcccaaccac cgaaaacgag ttggtccaag gttgcgatgg gcaagaaatc tttgagaagg
     9481 ggcactcgat tgtcggttcg ttttccgagg cggatggttc ccagttgcgt caacgaacct
     9541 atgggttttg cgaggccttt tcgtgcgagc aagcatccgg ccacgaacgt tgtggcgacg
     9601 gctccaaggc ctggaaggag aacgccaagt ttgccattgg ggggtgtaat gactgaaggt
     9661 ttcttcacgg taacctcaac gcgattcgat cgatttggta caaggatgag tctttcatcc
     9721 gtgtcggtgg cggctcgtgg caaatatggt gccgttggta ttcgtgggtg cgacgccgac
     9781 gagcggaggc tggagcctac gcctggttgt tccgtttgtg gagggctgtc ttactttggg
     9841 tgtggattcg ttcctgcggc atggagattg tcattcctcc cttggcgtgc tggcgcgaaa
     9901 cgtgtaagcc gcgcgccagc atgagcatac tcgatggcta caaagcgtcg ctcaagcctt
     9961 tggaagtcga agagccgatc gatgtgtacg tgcatcgacc gcttgggtac ttggttgcct
    10021 tattgacgtt tcgaacaccg atttcaccgg acttgatcac tctggcgtcg atcgtggccg
    10081 ggatcggcgc aggggtgtgt attgtatggc gttttccgta tcacctgccg gtggcggggg
    10141 cgttggtgtt tttgtccgcg gtgctcgatt gtgcggatgg gcaactggcc cgcatgcgaa
    10201 aaacatcgtc ggcattcgga aggatgatcg atggagtggc ggatttgatc gtgatgctgt
    10261 cggtggttcc cgcggcgttg gttcaggtgt ggcggttgta cgacaacgac ccgtggctgg
    10321 ctggcacagt ggtcggaatt agcatctttg cggtggtgac gagttcgttt catacgggca
    10381 tgtatgatca ctataaaaac gtatacttac atctggccca tccgtcttac agggatggtg
    10441 aggattacga gacggcgcgg gagcggtggg aaaagggagc ctcggggcag ccgttctgga
    10501 agcggctatc gtggcaagtg tatttttttt acatcaagag ccaggcgggt tacgtggccg
    10561 ggttcgatcc ctacacgagt gcaaggttcg cactatttcc cgcgtatgat ccgcaacgag
    10621 cagccgtata tcgagaacaa aaccttcgcg tgctgcgggt ttggcgaagc ctatttggat
    10681 ttggctcgtt ggtgttcgga accgcgatgt tcgtggcgtt gggaaggcca gacgtgtatt
    10741 tgttgtaccg tctgttgatt ctcaatggca ttttctatct ctatttgcgc cccgcgcaac
    10801 gtcgagcgtc acgccaggcg ttcgagcaaa tgggattgcg gatgccggat cagaaatgga
    10861 gtgacgggac gtgacgcaga aacgcggttg tgcgccggta gcggtggtgt tggtggcggg
    10921 cattggtgag cgtttgcatc cgttgacgtt gcatcggccg aaagcgctgg tgcaggtggg
    10981 cggcgagtcg atgctggcgc gaagcgttcg gctcttgctg gcgcatggcg tggagcaaat
    11041 cgtgttggcg accggtttca aggaggatgc ggtgcgtgag gcaatgcagg gcgtgccggt
    11101 tcccgtgtct tatcaattga acccgcgcta tcgagagacg caaaacgcgg tgtcattgct
    11161 ggcctgtgag caagcggtag ctggacgagc gttttacaag ttggatgggg atttgttgtt
    11221 tcgtgcagag gtgttggaac gattggatga ggtggacagt gggttggtcg tggcgatgga
    11281 ccgttcggtg gaacttgggc aagaggagat gaagattcgg ctaaagcagg gggggacatc
    11341 gatcgcggcg tttggcaagg ggctggagcc atcgtcgtgt cacggagaga gcatcgggat
    11401 tgagcgtgtc aatgaagtcg cggtgtcgtt gatctttcac ggtctgcgtc aggcggtgga
    11461 ggctggggaa acccatttgt attacgagga cgtctacggt cggttggtgg atcatggttt
    11521 ggaggcgagg ggggtgg
//
LOCUS       DAOSOR010000123        11536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5578_length_11536_cov_4.512586, whole genome shotgun sequence.
ACCESSION   DAOSOR010000123 DAOSOR010000000
VERSION     DAOSOR010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11536
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5578_length_11536_cov_4.512586"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PK710_08850"
     CDS             <1..872
                     /locus_tag="PK710_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09865.1"
                     /translation="LLVFALASLLLVGNASQVPFALQVGAAFGAAGPMIGLFVLLLLM
                     VANGRQAESRTDPRAVFAVLGLSAGFLSLMHNLSDPKHRPLYDNNPIIPLAVVSVFIL
                     LDQARATVLKHVVMGLMMLALFGGKYQRYLMARTPVQDPGFWRGLRVNHNGTILLEAA
                     QRARELAGPTGTVLTLPEDPMFQALVGRARPRLRGAIVFVDQFPEHVLEEDLAALRAD
                     PPTVLLLHPDADQGWQMVYSIWSTDSPAAKLQTRFLSEHRDTTYRVDRTFPTWLFDNG
                     VRMALLVRNDRLP"
     gene            919..1458
                     /locus_tag="PK710_08855"
     CDS             919..1458
                     /locus_tag="PK710_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09866.1"
                     /translation="MSNIKRTQFAQAAFDYATYFTATFGRTQIGGGCIRRPHLVAPDG
                     MSTAGGKLIRQHIVLRPDVPAFSTLTVGAVDVAKGAATLRTFGYLRHAHQARFGSRPF
                     DLDGVGYQTFFSQAQEFFARQGMRVDVETEANVPKSDVPTAPPDRAKTTILVVITLVV
                     LVILSAVAVGGYVLIRILG"
     gene            1673..3400
                     /locus_tag="PK710_08860"
     CDS             1673..3400
                     /locus_tag="PK710_08860"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT09867.1"
                     /translation="MDFLLISPPVSNFGQAAPALSVLTSFLRSKGWHACQWDLGIEAF
                     HHFYCEQTLRRSYQLLVAKDAEEVLLDLGQRVVSEIERAKETLRRPGVGADTNTMRWA
                     LHTIRDASVLLTAASGGGWELQDSKFDVPGALRSFSDLEQAYADPERNPFLSFFEEHA
                     AARLLRDRPRAVGISITYLSQLIPALSLVTVLKRVLPGTPVILGGAYLTATENEMSRI
                     PCSTLLADAVVLHDGEHALDLWLRHVLMGDPEPSHPNVWLRDSNRYRRTSFGACPQVD
                     LDSLPIPMWIADGLRLDDYLVPQYPIALPLSRGCYWGRCVFCNISSQTSSSYRRRSVA
                     NAIADIKAAMAETGSRWFDFPVDSFRPKHLRELALAIVQEGLSIEWGAEVLLDRGFND
                     EVLADLARSGCRCLRFGMESACPQTLRAMNKPVQPQTARRVLGSCRKHGIRTAVMFIV
                     GFPSETQAQIQQTYDFVVDNRDRIDFLTFHTYSLVLGSPMAEDPGKYGLYLKPGRAIF
                     SPNLPFVNTNPGGMQEDHVAQIVEPMRESLREFFPDLGELWTVGIGGWMTFANCCEAP
                     PAEPPVAQS"
     gene            3487..6630
                     /locus_tag="PK710_08865"
     CDS             3487..6630
                     /locus_tag="PK710_08865"
                     /inference="COORDINATES: protein motif:HMM:NF027656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOT09868.1"
                     /translation="MHRGLHLCLAACITSLSSSAFGHPSLYFDAAGLTQLKQLASEDA
                     DTGLGWNYAAVFQEIVKAADQVLASPYTYTVEIPNPNGQGSVTWTYSISSTMPPPHPN
                     NPGYPPWTKVSRDLQSRMEVLSFVYAVTGDSKYLSNAQGTGALDIAKAVSAWSKWTDP
                     GYLCGTGNSCLDTAHLLLGVGMTYDLGYGVMTDAERKTLREALIQKGVTPLAKDVNAA
                     NSASGGLAAWFNGYALRVTGLAVGACAVAEDVPAQSTPWTTLARQSVLAFFDSQGKDG
                     GAFEGHLYGAYAVDNLVIAAHVLDQKSLGSGLFDHGWLADLPRFAGAFLGSDNRSLAN
                     FGDSSTAAYWATTMFALAAHGNASAQWYLVQTGNSRPRSFLNMVWSRPTLTPTPLAGS
                     GTSLYQDVGYAVLRDGFQGAPVVAVKSGPPKEKVGHNHFDHNSFILSAHGHWIASDPG
                     YEDYFNPSKHLYTTGSIGHNTILVDKTVSSDGASVSKGQVSLTGGKLDFLFDGVAYAK
                     VVGQAAATYEAGLLDRFGRRIFYSKPDLVFIFDDLASSKAHEYSFLLHSGSAGKLTPG
                     QGPGELVSSLQTAMMQTFFASSVPLVSGYPRVTVHPGASDYGPYGEWRSQSYKAVRFA
                     AALVPGVQAHVTFDNPGFEDGLSSWQPRFADGTHSVDEAVAHSGKRSARIEHAVSKTG
                     YYYSEVMSLVPGSTLRASVFVKSKAATGSISIQPFFTLGGVYITDPAGKSISLDAKDP
                     VDWTEVVLQTQAPVTGVDGVRIALQFTGSGTVWFDDASYWVDTPPPQTEPSTAVVLGD
                     PPAGLAVSGPWGIDAAASFMGSGLTGTVTVQPATQAIAQLSEISTDGSIFCVGFEPKG
                     GLRRMYLQGGTFLSMGGKELLRASKSASFDMAIRRHSGCTVVELAEVETLEGPPYTVA
                     VVADEVWLGAKRVPFVVQGETTTFPEGSDLGPGCAVVDAGTESGADDASAVDDASGVE
                     SGVSFDAATDVSSQPGEDASEGGLQARGSAQDEASGCACRAHRFSRSGGSFGGWAAVA
                     LLLALGLRSKSRVRIAG"
     gene            6763..9381
                     /locus_tag="PK710_08870"
     CDS             6763..9381
                     /locus_tag="PK710_08870"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOT09869.1"
                     /translation="MARSTGIFLIALGLNHPTNAMAQTSPAPRSDESNPPSSEPSNVV
                     APRLEQFVSAPYPPEAQKKGIEGNVVLQLDISDTGQVSRAVVVNPAGHGFDEAATEAA
                     LRFRFSPAQRDGKPVAARILYRYNFTLQPVAAPSDVAAQEPTPLRNLSGQVRTSEGDV
                     PLAGALVTIRGSDGAEHEITTGEDGRWAFEDLAAGVYSVRVQAPGFEALEVQEQVDAD
                     ALTEVVYRVVTQGVLEVLIQGKRPAREVTKRTVEKREIEKIPGTSGDALRSIQNFPGV
                     ARPPGIAGMLIVRGSSPGDTDVFIDGTLVPLIYHFGGLSSVVPTELLERIDFYPGNFS
                     VQYGRVLGGIVDVGIRSPKSDGYHGMVQVDLIDTRALLEGPVPFVDGWSFAIAGRRSW
                     FDAWLGPVLREAGASVTAAPVYYDYQVIAETKPTARSSLRFGFLGSDDRLEILTREAS
                     EVDPILSGNIRMQTSFWNLFGRYENDLSDAIRLTSVTAWGSQILEFGVGTLYFELTNY
                     PFTNRTEVTYRTGARATVHAGVDLLWSEAQVKALVPQPPRAGEPDPGPFASKPPMATA
                     QDFTIFRPAGYVEIELTPQERLKLVPGMRADYARDIEAWDLSPRLNARYALIQEYPST
                     TLKGGIGLFHRPPELNESSPPFGTPGLRSNRSIHYSLGVEQDISRKVDVSVEGFFKRQ
                     DLLVGAAPSETGLFEYNNLGRGYVIGSEMLLRYKPDERFFGWIAYTLSRAVIRDQPDR
                     EARLANFDQTHILTALGSYKLGRGWEFGARFRLVSGNPETPIVGSLYNANSGSYAPLE
                     GTINSRRVEPFHQLDLRVDKMWKFEAWKLSAYLDVQNVYYSQSVEGYQYNFNYTQRTA
                     VTGLPIIPSIGLRGDF"
     gene            9381..10412
                     /locus_tag="PK710_08875"
     CDS             9381..10412
                     /locus_tag="PK710_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09870.1"
                     /translation="MMGSAKQAIAKQARFPRWMRYALTASVWGSLLGGGIAVTGCEEG
                     FDPPSKLNTLRVLGVQADKPYPKPGETVELRMLWHDGKSPVDAPRPVQIVWIGGCFNP
                     AGDLYYLCYPQMAEKLSQIEKDPSLIDKLFGFGDTFSLDIPADIIANRPAVPGAEPYG
                     LSYVFFAACAGQLAPSEPGDDGLPFGCFDETGRRLGADDFVPGYFGLYSFADRTNDNP
                     IVVGLMINGHLIDPSQEPSFPRCTKGACHDLQLRAVVDPESAETNTGLVDSDGRVLSE
                     QMWVEYLATAGKIERSPRLVNDASKGFNEDNGTAYEPPSEVGKQYLFAVVRDNRGGVA
                     WVKQGVWFE"
     gene            10557..11315
                     /gene="pyrH"
                     /locus_tag="PK710_08880"
     CDS             10557..11315
                     /gene="pyrH"
                     /locus_tag="PK710_08880"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOT09871.1"
                     /translation="MSIRMTIQGKPQPVHPDLKIHRVLLKLSGEALGGPDGAGLDSIV
                     MRTVSLELKEIFELGVQLGIVVGGGNIFRGLKGAAKGMDRAQADHMGMLATVINSLAL
                     QDALESVGVPTRVLSALEILEVAEPYIRRKAMAHLERGNVVIFAAGTGSPFFSTDTAA
                     ALRAVEMQADCVLKATKVDGIYDKDPVQHKDAKRYATLTYDRFLAERIGVMDATAVSL
                     CRENAMPIRVFSMTTEGNFRRVCMGDDVGTTVVD"
BASE COUNT         2156 a         2910 c         3743 g         2727 t
ORIGIN      
        1 ttttgctcgt atttgccttg gcatcgctgt tgttggtggg aaacgcatcg caagttccgt
       61 ttgctttgca ggtgggtgcg gcgtttggtg ccgcggggcc gatgatcgga ttgttcgtgt
      121 tgctgctatt gatggtagcc aacggacgac aggcggaatc gagaacggat cctcgagcgg
      181 tatttgccgt cctgggattg agcgcggggt tcctttcgtt gatgcataat ttatccgacc
      241 ccaagcatcg tccgttgtac gacaacaacc ccatcattcc gttggccgtc gtatcggtgt
      301 tcattttgtt ggaccaggcg cgagccacgg tgctcaagca cgtcgtgatg ggattgatga
      361 tgctggcgtt gtttggggga aaatatcagc gatatctgat ggctcgcacg cccgtgcagg
      421 atccaggatt ttggaggggg ttgagagtca accacaacgg gacgatattg ctcgaggcgg
      481 cgcaacgggc cagggagttg gcggggccga ccggtactgt cctgacgtta ccggaggatc
      541 cgatgtttca ggctctcgtc gggcgtgctc gtccgcggct tcggggtgcg attgttttcg
      601 tcgatcaatt tcccgagcat gttctcgaag aggacttggc ggcgcttcga gcggatccgc
      661 ccacggtgtt attgcttcat cccgatgccg atcaagggtg gcagatggtc tacagcattt
      721 ggtctacgga ttctccggcg gccaagcttc agacgcgatt tttgagtgag catcgagaca
      781 cgacctatcg tgtggaccgc acgtttccca cgtggctttt cgacaatggg gttcgcatgg
      841 ccttgctggt gcgcaacgac cgcttgccct gatccttggc cgtcgggcca caccttccgt
      901 tacaattgcg tcatccgcat gtcgaacatc aaaagaactc agttcgccca agcggcattt
      961 gattacgcaa cctatttcac cgctacgttt gggcggacgc agatcggagg ggggtgcatt
     1021 cgtcgtccgc atttggtggc gccggatgga atgagcacgg cgggtggaaa gctcatccgt
     1081 cagcacatcg ttttgcgtcc tgatgtgccc gcgttttcca cccttacggt gggcgcggtg
     1141 gacgtggcga aaggcgcggc aacgcttcgg acgtttgggt atctgcgcca tgcgcatcaa
     1201 gcgcgttttg gcagtcgtcc gttcgatctc gatggtgtgg gttatcaaac atttttctcc
     1261 caagcgcagg agttttttgc gcggcagggg atgcgtgtcg atgtcgaaac ggaggcgaac
     1321 gtgccgaagt cggacgtgcc cacggcacct ccggaccgcg caaaaaccac tattctcgtg
     1381 gttatcaccc tggttgtcct tgtcattctc agtgccgtgg ccgtaggggg ctacgttctc
     1441 attcgtatct tgggttgaat ggcctcctcg atcgattatc catatccgta taattaatat
     1501 cattcgtcat gcgatgccgt tcttgacgat cgggagccga acgctgtggc aagatgggcc
     1561 atgatgggtc agcgattcat ggcccgggtg atttgaaaac cgctcctgtt gtgcgcaatg
     1621 gctcgctggg gattgacgtt gcgcgctcta ggagtagagc agcggaaacg acatggattt
     1681 tttgctcatc agtcccccgg tgtccaactt cgggcaggcg gctccagcgt tatcggtatt
     1741 gacgtcgttc ttgcggtcca aaggttggca cgcctgtcaa tgggatcttg gtatcgaggc
     1801 ttttcatcac ttctattgtg agcagacgct tcgacgaagt taccagcttc tcgtagccaa
     1861 ggatgcggaa gaggtgctgc tggacttggg tcagcgtgtg gtgagcgaaa tcgaacgtgc
     1921 caaggaaacg ttacgacgtc ctggggtggg agcggatacg aatacgatgc gttgggcgtt
     1981 gcatacgatt cgtgatgcca gtgtgttgtt gacggcagca tcggggggag gatgggaact
     2041 gcaggacagc aagttcgacg tgcctggagc gttgcgtagc ttctccgatc tcgagcaggc
     2101 gtatgccgac ccggagcgca acccattcct ttcgtttttc gaagagcatg cggcagcacg
     2161 attgctgcgt gatcgtcctc gcgcggtcgg catctcgatc acgtatttgt ctcaactcat
     2221 tccggctctg tctttggtca cggtgctgaa gcgggtgttg cccggaacac cggtgattct
     2281 tggtggcgcc tatttgacgg ccacggaaaa cgaaatgagc cgaattcctt gcagcacgct
     2341 tctggccgat gcggtggtgc tgcacgatgg ggagcatgcg ttggaccttt ggctgcgtca
     2401 tgtgcttatg ggtgacccgg agccttccca tcccaacgtt tggttacggg attcgaatcg
     2461 gtatcgccgc accagctttg gtgcctgtcc ccaagtcgac ttggattcgc ttcctatccc
     2521 tatgtggata gccgacggtc tgcgtttgga cgactacctc gtgccgcaat acccaattgc
     2581 tcttcccttg tcccggggat gctattgggg gcgctgcgtg ttttgcaata tctcgagcca
     2641 gacttcttcc agctatcgtc gacgatccgt ggccaatgcg attgccgaca tcaaggcagc
     2701 gatggcggag acgggttcac gatggttcga tttccccgtg gactcgttcc gacccaagca
     2761 tttgcgggag ctggcgcttg cgatcgtgca agaggggttg tcgatcgaat ggggcgcgga
     2821 ggtgttgttg gatcgtgggt tcaacgatga ggtgctggcg gacctggctc gttcggggtg
     2881 tcgatgcctt cgattcggga tggaaagcgc gtgtccccag acgcttcggg cgatgaacaa
     2941 gccggttcag ccgcagacgg cacgccgggt ccttggttct tgtcgaaagc atggcattcg
     3001 cacggcggtg atgttcatcg tgggatttcc ttcggagacc caagcgcaga ttcagcagac
     3061 gtacgatttc gtggtcgaca accgggatcg gatcgacttt ttgacctttc atacgtattc
     3121 gctggtcctt ggctctccga tggcggaaga cccgggaaag tacggcctgt atctgaagcc
     3181 gggacgagca attttttccc cgaatctacc cttcgtgaac accaatcccg gcggcatgca
     3241 agaagatcat gtcgcccaga tcgttgaacc catgcgcgag tccttgcgag agttcttccc
     3301 cgatttgggt gaattgtgga ccgtgggcat tggtggttgg atgacgtttg ccaattgttg
     3361 cgaagcgcca ccggccgagc caccagtcgc gcagtcatga accatgggcc gacgcgcgga
     3421 ctggctcggc gatcgcgcaa cgcaaccata gctatgtttc ttctcgaggc gtgataccct
     3481 tggttcatgc atcggggcct tcacctctgc cttgcggctt gtatcacaag cctgtcatct
     3541 tccgccttcg gccatccctc tttgtatttc gatgccgcgg gtctaacgca actgaagcaa
     3601 cttgcttcgg aagacgcgga cacgggattg ggatggaact acgctgctgt ttttcaggag
     3661 atcgtgaaag cagcggacca ggttttggcg agcccctaca cgtacacggt ggagattccg
     3721 aatcccaatg gtcagggttc ggtaacgtgg acctattcga tttcttcgac gatgcctccg
     3781 ccgcatccca acaatcccgg ctatccgcca tggaccaaag tgtcgcggga cttgcaatcg
     3841 cgcatggagg tgttgtcgtt cgtgtacgcg gtgacagggg acagcaagta tctttcgaac
     3901 gcgcaaggaa cgggagcgct ggacattgcc aaggcggtgt cggcttggtc gaagtggacg
     3961 gatcctggtt acttatgtgg aacgggcaat tcttgtctcg atacggcgca tttattgctc
     4021 ggggtgggga tgacctacga tcttggttat ggggtgatga cggatgcgga gcgcaagacg
     4081 cttcgtgagg cgctcatcca gaagggggtg acgccgttgg cgaaagacgt gaatgcggcg
     4141 aactctgcct cgggtggatt ggcagcgtgg ttcaatgggt atgcgctgcg ggtgacggga
     4201 cttgcggtgg gcgcttgcgc cgtggcggaa gatgtcccgg cgcaatcgac tccgtggacg
     4261 acgcttgcgc gccagtcggt tctcgctttt ttcgattctc agggcaagga tggtggcgcg
     4321 ttcgaagggc atttgtacgg cgcgtacgcg gtggacaacc tggtgatcgc cgcgcatgtt
     4381 ctcgatcaaa agtcattggg gtccgggctt ttcgaccacg gttggcttgc cgatcttccg
     4441 cgtttcgccg gggcatttct tggcagtgac aaccgcagtt tggcgaactt cggggactca
     4501 tcgaccgcgg cgtattgggc aacgaccatg ttcgcgctcg cggcccacgg caatgcttcc
     4561 gcccaatggt atctcgtgca aaccggcaac agcagaccgc gcagttttct caacatggtg
     4621 tggtcgcgtc ccacgctcac gccaaccccg ttggccggct cgggaacgtc gctgtatcag
     4681 gatgttggct acgcggtcct tcgcgatggg tttcaaggcg ctccggtggt tgctgtcaaa
     4741 agcgggcctc ccaaagagaa agtcggacac aaccacttcg atcacaatag cttcatcctc
     4801 agcgcccacg gacattggat cgcttccgat cccggctacg aagactactt caacccttcc
     4861 aaacatcttt atacgaccgg ttcgattggt cataacacca tcctggtcga caaaaccgta
     4921 tcgtccgatg gcgcatcggt gagcaagggt caggtctcgc ttacgggtgg caaactcgac
     4981 ttccttttcg atggtgttgc ctatgcgaag gtcgtggggc aggcggcggc gacgtacgaa
     5041 gcgggattgt tggacagatt tggccggagg atcttctata gcaaaccgga tttagtgttt
     5101 attttcgacg acttagcatc ttcgaaggcc catgaatact ccttcttgtt gcattcgggt
     5161 tctgcaggaa agctgacgcc gggacaggga cctggggagc ttgtaagttc gcttcagact
     5221 gcgatgatgc aaacgttttt tgcctcgagt gtgccgcttg tttccggata ccctcgggta
     5281 acggttcatc cgggtgcttc ggactatggg ccttatggcg agtggcgaag tcaatcgtat
     5341 aaagcggttc gctttgccgc ggcgttggtg cccggcgtgc aggcgcacgt tacgttcgac
     5401 aatcctggat tcgaagatgg gttgagcagt tggcagccgc gttttgcgga tggcacgcat
     5461 agtgtggatg aggctgttgc gcattcgggc aagcgaagcg ctcgcatcga gcatgccgtt
     5521 tcgaagacgg gctactatta ttccgaggtg atgagtcttg tgcctggctc cacgttgaga
     5581 gccagcgtgt tcgtgaagtc caaggcggcg acgggatcga tttcgattca gccatttttc
     5641 acgttgggtg gtgtgtacat caccgatccc gcggggaaat cgatatcgct cgatgccaag
     5701 gatcccgtgg attggactga agtcgtgttg caaacgcaag cgccggtgac gggcgtggat
     5761 ggtgtgcgaa ttgccttgca attcacgggt tcgggaacgg tttggttcga tgacgcctcg
     5821 tattgggtcg atacgccacc gcctcagacg gaaccatcga ccgcggtcgt gttgggggat
     5881 ccccccgcgg gtcttgcggt ttctgggcct tggggaatag atgcagccgc ttcgttcatg
     5941 ggctcggggc tgacgggaac ggtgacggtt caacccgcga cgcaggcgat tgcgcagcta
     6001 tcggagatat cgacggatgg ttcgatcttt tgcgtgggat tcgagccaaa agggggattg
     6061 cgccggatgt atctacaagg gggaacgttc ttatcgatgg gcggaaagga attgctgcgg
     6121 gcttccaaga gcgcttcttt cgacatggca attcgtcggc actcgggatg cacggtggtg
     6181 gagcttgcgg aagtggagac tctcgagggg cctccgtata cggttgcggt ggtggccgat
     6241 gaagtatggc ttggggcaaa gcgtgtgcct tttgtggttc agggtgaaac gaccacgttt
     6301 cccgaaggtt cggatcttgg gccaggctgt gcggtggtgg atgcggggac ggagtctgga
     6361 gcggatgacg catcggcagt ggatgacgca tcgggagtgg aaagtggggt gtctttcgat
     6421 gcggccacgg acgtttcctc gcaacccggg gaagatgctt ccgagggtgg tttgcaggct
     6481 cggggcagcg cgcaagacga agcttcgggc tgtgcctgtc gagcgcatcg tttttcccga
     6541 tcgggcggca gtttcggtgg ctgggccgca gttgccttgc ttttggcgct gggattgcgg
     6601 tccaaaagcc gcgttcgcat cgcaggctag cgcgtttggc gtgaacccat tataccgctc
     6661 gggtctatcc tcgagcacga cgtggcgttt tccgatctcg gatccaggag ttcgatgcaa
     6721 tccaccgcgc gcagcaaacc aacccatttt tcgcgccgcc ggatggcacg atcgacaggt
     6781 attttcctga ttgcgctcgg cttgaaccac ccaacgaatg cgatggcaca aacgtcgcca
     6841 gcgcctcgaa gcgatgagtc caacccacca tcgtcggagc catcgaacgt cgttgccccg
     6901 cgactggagc aattcgtttc cgcgccgtat ccgccggaag cccagaaaaa gggcatcgaa
     6961 ggaaacgttg tgttgcagct cgacatttcc gacacgggac aagtgagccg cgcggtggtc
     7021 gtcaatccag cgggtcatgg ttttgatgaa gcggccacgg aagcggcgtt gcggtttcgg
     7081 ttttcaccgg cgcaacgcga tggcaagccc gtagccgcgc gcatcttgta ccgttacaac
     7141 ttcacgctgc agccggtagc tgcgccatcg gacgtggcgg ctcaggagcc gactcctttg
     7201 cgcaacttgt cggggcaagt acgaacgagt gagggagatg tgccgttggc gggagctttg
     7261 gtgaccattc ggggcagtga tggcgcggag catgagataa cgacggggga agatggtcga
     7321 tgggcttttg aggatttggc cgcgggtgtg tattccgtgc gggtgcaggc accggggttc
     7381 gaggctctcg aggtgcagga gcaagtggat gcggatgccc tgacggaagt cgtgtatcgg
     7441 gtggtgacgc aaggcgtgct cgaggtattg atccagggca aacgtcccgc gcgagaggtg
     7501 accaaacgca cggtcgaaaa acgagaaatc gaaaagattc cgggtacttc cggtgatgcc
     7561 ctgcgatcga ttcagaactt tcccggcgtg gcgcgtccgc ctgggattgc aggcatgttg
     7621 atcgtgcgcg ggtcatcgcc tggcgatacg gatgtgttca tcgacggcac cctcgtgccc
     7681 ctcatctatc attttggggg gttgtcttcg gtggtgccca cggagttgct cgaacggatc
     7741 gacttttatc caggcaactt cagtgtgcaa tacgggcgtg tgctgggagg catcgtggat
     7801 gtggggattc gatcgccgaa aagcgatggg taccacggca tggtgcaggt ggatctgatt
     7861 gacacgcggg cgctcttgga gggtcccgta ccgttcgtcg atggctggag ttttgcgata
     7921 gcgggacgtc gcagttggtt tgatgcctgg ctggggccgg tgcttcggga ggcgggggca
     7981 tcggtgacgg cagcaccggt ctattatgat taccaggtga ttgcggaaac gaagccgacg
     8041 gctcgatcgt cgttgcggtt cgggtttttg ggttcggacg atcggctcga gattctcacg
     8101 cgagaagcgt cggaggtgga tccgattttg tcgggcaaca tacgcatgca gacgagcttc
     8161 tggaatctgt ttgggcggta tgagaacgac ttgtcggatg ccattcgtct tacgagcgtg
     8221 acggcgtggg gctctcagat tctcgagttt ggtgtgggca cgttgtactt cgagctgacg
     8281 aactatccgt ttacgaatcg gacggaggtc acgtatcgaa cgggggcgcg ggcgacggtg
     8341 catgcgggtg tggatttgct gtggagcgaa gcgcaggtca aagcgttggt tccgcaaccg
     8401 ccgcgtgcgg gagagccgga tccggggccg tttgcttcga agccgccgat ggctaccgcg
     8461 caggatttca cgatctttcg accggccggt tatgtcgaaa tcgaactcac gccccaagag
     8521 cgtttgaagc tcgttccggg tatgcgcgct gattacgcgc gagacatcga agcgtgggat
     8581 ctgagtccgc gtttgaatgc tcgttatgca ttgattcaag agtacccgtc gaccacgctc
     8641 aaaggcggga tcgggctttt tcatcgccca ccggaactca atgaatcgtc gccgcccttt
     8701 ggtacgccgg ggcttcgatc caatcgctcc atccattatt cgctgggtgt cgaacaagat
     8761 atcagccgaa aagtcgatgt atccgtggag ggattcttca agcgacagga tcttctcgtg
     8821 ggggctgctc cttcggaaac gggtctgttc gaatacaaca acctgggaag aggctacgtc
     8881 atcggcagcg agatgcttct gcgttacaag cctgacgagc gattttttgg atggattgct
     8941 tatacccttt ctcgtgctgt gattcgggat caaccggacc gtgaagcgcg tttggcgaac
     9001 ttcgaccaaa cccatatcct cacggctttg gggtcgtata agcttggacg gggatgggaa
     9061 tttggcgcac gatttcgctt ggtttccggc aatccggaaa caccgatcgt cggctctcta
     9121 tacaacgcca actcgggctc ttatgcgccg ctggaaggaa cgatcaactc gcgtcgtgtc
     9181 gaaccgtttc atcaactcga cctgcgagtc gataagatgt ggaagttcga ggcgtggaaa
     9241 ctgtcggcgt acctcgacgt gcaaaacgtt tactactcgc aaagcgtgga agggtaccaa
     9301 tacaacttca attacacaca acgcacggcg gtcacggggc tgccgatcat cccgagcatc
     9361 ggcttgcgag gagacttctg atgatgggtt cggcaaagca agcgatcgcg aagcaagcgc
     9421 gtttcccacg gtggatgcga tatgctctga ccgcatcggt atgggggtcg cttttgggag
     9481 gcgggattgc cgtgacggga tgtgaggaag ggttcgatcc tccgagcaag ctcaacacgc
     9541 ttcgagtgct tggcgtgcaa gcggacaagc cttatcccaa gccaggcgaa acggtggagt
     9601 tgcgaatgct ttggcacgat ggcaagtcgc cggtggacgc gcctcgaccg gttcagatcg
     9661 tgtggattgg tggttgtttc aatcccgcgg gggatcttta ttacctttgc tatccgcaga
     9721 tggcggagaa gctctcgcaa atcgaaaaag acccttcgct catcgacaaa ctatttggat
     9781 ttggggatac cttttctctc gatatccccg cggatatcat tgccaaccgt ccagcggtcc
     9841 caggggctga gccctatggg ctttcctacg tatttttcgc ggcttgtgcg ggtcagctcg
     9901 cgccttcgga gcctggggac gacgggttgc cttttggttg tttcgatgaa acgggtcgtc
     9961 ggcttggggc agacgacttc gtgcccggct atttcggttt gtattcgttc gccgatcgaa
    10021 cgaatgacaa ccccatcgtg gttggtctca tgatcaatgg ccatctcatc gatccttcgc
    10081 aggagccttc attcccacga tgtacgaagg gcgcgtgtca tgatttgcag ctcagagcgg
    10141 ttgtcgaccc ggaaagcgcg gaaacgaata cggggttggt ggatagcgat ggacgcgtgt
    10201 tgtccgagca gatgtgggtg gagtatctgg ccaccgcggg gaagatcgaa cgcagtccgc
    10261 gtctggtgaa cgatgcatcc aagggattca acgaggacaa cgggacggct tacgagccgc
    10321 cttcggaagt gggcaagcaa tacttgtttg cggtggtacg ggacaaccgg ggtggggtgg
    10381 cgtgggtcaa gcagggggta tggttcgaat gaacgagggg gcgatctttg taaaatgttg
    10441 aatttacatg attatttgta gggcggaccc atggcatttc caacaagggt tctggttgtg
    10501 caggggttgg cagagtcggg cttgtgtcat gcgctcggtg ctgataccgc cggaccatgt
    10561 cgattcgcat gaccatccag ggaaagcctc agcccgttca tcctgatttg aagatccatc
    10621 gtgtgttgtt gaagctgagt ggggaggcgc ttggcgggcc ggatggggcg gggctcgatt
    10681 cgattgtgat gcgcacggtg tctttggaac tcaaagagat ttttgaattg ggggtacagc
    10741 ttggaatcgt cgtgggaggg ggcaatattt ttcgtgggtt gaaaggtgcg gccaaaggca
    10801 tggatcgggc gcaagccgac cacatgggca tgcttgcgac ggtgatcaat tcgttggcgt
    10861 tgcaggatgc gctcgaatcg gtgggggtgc ccacgcgtgt gttgtcggct cttgagattc
    10921 tggaggtagc cgagccgtat attcgtcgca aagccatggc gcatttggag aggggcaatg
    10981 tggtcatttt cgcggcaggg acaggcagtc catttttttc gacggatacg gcggcggcgt
    11041 tgcgtgcggt ggaaatgcaa gccgattgcg tgctcaaggc caccaaggtc gacggcatct
    11101 acgacaagga tccagttcag cacaaggatg ccaaacgtta tgccacgctg acgtacgatc
    11161 gattccttgc ggagcgcatt ggtgtgatgg atgccacggc ggtgtctcta tgccgcgaaa
    11221 acgcgatgcc gatccgtgtg ttttccatga cgacggaagg aaattttcgt cgtgtatgca
    11281 tgggcgacga cgtgggtacg acggtggtgg actgaggtct agcgagatgg ggccaaggga
    11341 tcgcagggcc gcgagggggg atttgggttt tccaggacgt tgactggatg cgttctattc
    11401 tgagcttaga cggcggatga ggacctgcta aggagcctgc cgtgccgcat catggaacat
    11461 ggttccgcat gcgggggcca tccgtggctc tcgatggatg atgaatcggt cttcgcgcta
    11521 tcgaccgctt cgatcg
//
LOCUS       DAOSOR010000124        11473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5619_length_11473_cov_3.929410, whole genome shotgun sequence.
ACCESSION   DAOSOR010000124 DAOSOR010000000
VERSION     DAOSOR010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11473
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5619_length_11473_cov_3.929410"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PK710_08885"
     CDS             complement(<1..547)
                     /locus_tag="PK710_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09872.1"
                     /translation="MPQFRHLFAVLSLIGLSSLSACDKSKQDERRGLPPPETSAATPG
                     GCSQGGGTLTDPSTATQFPRSFSDYCIDPHGETRTYGASASKDIDAICTEAFNGDCEM
                     YKSFGLERVILFRYIDGGGSPGAVDVVVSKYASSEGAFGMFTKRVISDGDPARQDAPK
                     EMLVEGMGALGAGTAYLWKAQI"
     gene            764..1873
                     /gene="gcvT"
                     /locus_tag="PK710_08890"
     CDS             764..1873
                     /gene="gcvT"
                     /locus_tag="PK710_08890"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOT09873.1"
                     /translation="MNDTILRKTPLYEEHKKLGARMVPFAGFEMPIQYEGLTQEHHAV
                     RNAVGIFDVSHMGELVLTGEHSESVLNYLVTSDVTTLVDGQAMYTCACNEKGTILDDL
                     LVYRHGKDRWMVVCNASNHDKIASHFAKAAKDHCGFEDQSPDTALVAVQGPKTFDVLA
                     QLSPEGARCSDLKAFHFGSYELAEIPCMVARSGYTGEDGVEIFCANRYAPKLWSLLLD
                     KGQAFGLKPAGLGCRDTLRLEARLSLYGNDIDETTNPLEAGLGWIVKLDGDDFIGRDA
                     LVEIKRQGLTRKLVGFEMVGRGIARHGYPLLDLDGNTVGVCTSGSPSPTLGKNIGLGY
                     LLSSMTKVGTRFKVDCRGKLIEAVVVKTPFYKRAG"
     gene            1902..2297
                     /gene="gcvH"
                     /locus_tag="PK710_08895"
     CDS             1902..2297
                     /gene="gcvH"
                     /locus_tag="PK710_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOT09874.1"
                     /translation="MSTFPNDRRYTVDHEWAKQEGGSVVIGVSSFAVEQLGDITLVSI
                     DASVGDHLEAGKAFGTIESVKTLSDLLAPISGILEAVNGELEDSPETINDDCYGKGWM
                     IKIKPASSADLDKLMDDAAYETHVKSSPH"
     gene            2323..3681
                     /gene="gcvPA"
                     /locus_tag="PK710_08900"
     CDS             2323..3681
                     /gene="gcvPA"
                     /locus_tag="PK710_08900"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOT09875.1"
                     /translation="MRYLPHTQQEIEQMLAAIGVPTVDALFDTIPAESRLGRPLALEP
                     GLDEVSLMRHLSDLAAGNQGATMLSFQGAGIYDHHIPPAVDQLLLRSELYTAYTPYQP
                     EVSQGTLQSIWEFQTIVSEILGHPVANASMYDGASAAAEAALMARRITKRSKIIVCDG
                     VHPEYIETIETYMSGLEGGRAHVIRTRVGDEGVTSLASLGDRIDDQVAAVVVGYPNFF
                     GCVTDLRPLAERIHAAGALLVTVTQDPYALALIEPPGHLGADISIAEGQAIATSPSFG
                     GPGVGLFASRIDRKYLQEMPGRLVGETVDANGKRGYVLTLATREQHIRRERATSNICT
                     NQGLVALSLTIRMCLLGKQGFVDVARQCLAKAKYLREQIASLEGYSIVYPNTASFHEF
                     TIRVPGQATTVVERLARDGILAGVPLSRYLPSRDDQLLVAVTEKHTRQDLDRLVSALR
                     AI"
     gene            complement(3684..4010)
                     /locus_tag="PK710_08905"
     CDS             complement(3684..4010)
                     /locus_tag="PK710_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09876.1"
                     /translation="MRIIRREPLSVHCEGTVQPERVANYLRQNVVSKTVEIGSVKTIF
                     EGALVKGSTAPLLRIEVEKVDRGTRLVVRNLTSPKADPFLTPEDAWKHYGFDGEGKRF
                     DPRKLE"
     gene            complement(4233..8354)
                     /locus_tag="PK710_08910"
     CDS             complement(4233..8354)
                     /locus_tag="PK710_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09877.1"
                     /translation="MTHTKLRILASLAAVVTLQTVGHPAKAQSAEYQAADVLLLLDTS
                     GSMAYTTKRSTTDTTRYALPQCGVYPASSPMSFDSAASFSSGPTPADRWAILANAFTG
                     TVIGAECSAQERSNTDFAGEYKLGAADPYDKGYYLPFNRIVATYTVNGTKQRCTPAPS
                     WDTDLRTALTNGNAMKWPDKAPVYWRDMSQTYSSGVECPFMPQESDGVIDRLQTSVRF
                     GIMTFDPSPSRVDTGGIGTGILSTFLPDFASGVGDTWSYFPGWLTTDTQYAGQGWPVD
                     CPPPPGAARLFEVGARNPAAPPWEGRLIGFGDPNATTEEIIAHNERVQQAILGIRPFG
                     GTPVAGMLKDALFFLTQDNTKPVDIGGASFYGGAVDPNVSMDRTHPGCRKRMAILITD
                     GGPNLDLRPHCDLKATPSTNDGKCPFETPQSYAKDLYDKNIDLFVVGFAVTAAKGSSA
                     LTCSQLMNIKDPEYRACSSLPPCTSTCAANQCAYGYCISTPEDELLATCCTIEGIANE
                     GSRYTAEDKGMTLGAPRSAYWAESGTELLAAISNILRFPPAAMTRTQPVFATGNISAT
                     KISDTIVGARFFSSFDPVSKDLYAGHLVRERYTCESNIPEEQEIDKSKGDYFEANVDG
                     NLSSRYIYTVLGDAYSGGTIHSTRSIRPMLPPSADPDGLGSVGGTTVEDSASVFASKV
                     TPTALFGTNQCDSAGQLCCARTSANTIPTATACRDRFLKLQLGLSATGIDYLRSSAFG
                     AMINSTPELVMPPSENLRDESYTLFQGLFSKRVPTIYAATADGQLHAFAINKQDNSLN
                     ELWSFIPPAVLPSIGEQYPTRYEAANKTLLSGPIVVGEVAGDASPTAGRFLTRTAPTP
                     SSFNTAKWYTVLLGTYGSSQGYFALDVTNPDRDETKKVTGYTSGPRFLWQLTTDKDGN
                     PLFGSRSTRPAIATLYFVMPGSGVGTLPARHAVAILPGGYGGVRSNDETNLETTTFPT
                     GSVANIVGVTHRTKTAKYTPVSNNPQDVLSLAGARSLTIVRLDTGEVIRTFRRSDSYN
                     AAAPEAPQALYGTNSGNSRVTKAPFAAPLVGHIISYPNTPGSVADRAYIGDAEGRLWR
                     LDLSSAEPQDWSVTLVHDAFPTDTTWGGPFGLADVGPIETPPILSTDPMGRVTIALST
                     GEQTSLTPVGRNSVWSLTEQKNLKTGDIQMDVNWILNASNAKNTTSTSDKTVHFAAGS
                     GERVTGPMALFSSVLYFTSFNPQTIDPGSCSPGSSYLWGVDYLHAGQTVGASGPTTAV
                     DGPLPQFPDPTSSTPANNLRVISFNEAGIAFGVGVMQKPSCITIEDDLSEDPFLGMTG
                     HQTIKSLNPGAFQLVVQVGANQGGGDKKVRVDTFDLLPPSARSVIDSWAAILD"
     gene            complement(8384..9052)
                     /locus_tag="PK710_08915"
     CDS             complement(8384..9052)
                     /locus_tag="PK710_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09878.1"
                     /translation="MVSQSAGRCFPPASLAVFKRRRQQRGASIFVVMSVITVLTAVGI
                     FAAHTAGLNQRISGYAKQSTQSAYIADLGTVAVVDELSGGKALAYMQLVMAGNESCRA
                     NQYITGISALPCYRLTKTDIENRMKTESGADYLIDSSGTSLSSPMALLRGDFLVELTD
                     PGPVGRPVAGMDQSGIGPRFRYMQLTFSGTGVVRPDDTNQQSVRLAAIRANRAIVQVG
                     PLPR"
     gene            complement(9046..10368)
                     /locus_tag="PK710_08920"
     CDS             complement(9046..10368)
                     /locus_tag="PK710_08920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOT09879.1"
                     /translation="MRVRRMSRQSDSRQTSKRGFTLIELLVAIMAGLLVATAAVSFSR
                     QATRFFAQESRIASAQMSVLAGFQRLQADLARSAYMAPTDMARDYAANKICAPNYDTW
                     PGSVKELTSIRISSATSKPDVVRVAGNLDSSEMFPVLAIAAKGTGHEVFLQTNNGPMT
                     RAGFIPSASDRDAAFQAVFRPGRLIRILDDQGKYEYEIIKSSSYSASPVITTVGRLPL
                     RSEISMASGATSASCGLSDGLGTQINPISVVEYRIANLSALSPYKDTIYHENYRVADS
                     DANRMELVRSEVLLLESSGSGSGGAGGAGGAGGSGSTLPSLTEQPEIVAEFAVDLKVG
                     VWTTSTASGGVVRGNLRYLAPGSAAGATLPTVALAPVGGPTAIRALDLRLVVRSREAD
                     RSETIDEVANPLVSDGYMFRSKVSEGRWARTRTLTATISLMNHRGEAW"
     gene            complement(10365..10979)
                     /locus_tag="PK710_08925"
     CDS             complement(10365..10979)
                     /locus_tag="PK710_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOT09880.1"
                     /translation="MNARRNNRRGSTVIEVMMAITVLAIGASGVVALQKVTSSANRNS
                     RTIAVANEVARTWIERLRMDAMLWNYPSPTNPTSDLNVDTLWLKNHIKGPDPTKTDWF
                     RPTNGNDICGTHDVFGRDEPCSGGATVQGPYCVNLRLSWVREDQRMIRAEVRVHWVGR
                     NEGDGAVDQKDLPCGDPATVDDLAAKGIFRVAHAVTMVTKNEAP"
     gene            complement(10979..>11473)
                     /locus_tag="PK710_08930"
     CDS             complement(10979..>11473)
                     /locus_tag="PK710_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09881.1"
                     /translation="LVRYSNGEFKVREAVVGKSSASSSGGGAGGAAGGGSTTNYFPVA
                     SCTMPATRWDDSSDLFEEIESFTLGGQAPYDLVKTEFSIYSAQSGATATVIGDSEVGD
                     VCFTPSGTMLFRKGTTTAFSSSPRTLMIRVGQMSGTTEVGISRVVFILPNGMARIAAK
                     VKES"
BASE COUNT         2528 a         3275 c         3157 g         2513 t
ORIGIN      
        1 cgatctgcgc cttccacaaa tatgcggtcc cggctccaag agcacccatt ccctccacga
       61 gcatctcctt cggagcgtct tgtcgcgcag gatcgccatc cgaaatgacg cgtttcgtga
      121 acatcccaaa cgcgccttcc gacgacgcgt attttgacac gaccacgtcg acggctcccg
      181 gtgacccacc cccatcgatg tatcggaaga gtatcacccg ttcgaggccg aacgatttgt
      241 acatctcaca gtcaccattg aaagcttccg tgcagatggc atcgatatcc ttgcttgcac
      301 tggcgccgta ggttcgtgtt tccccatggg gatcgatgca ataatcagaa aaactgcgag
      361 gaaattgcgt tgctgtcgaa gggtccgtca gcgttcctcc tccctgagaa caccctcccg
      421 gcgtggccgc ggacgtttct ggaggaggaa gccctcgccg ctcatcctgt ttcgacttgt
      481 cacatgcgga aagagacgac agcccgatga gagataacac tgcgaaaaga tgacgaaatt
      541 gtggcatgag gactccggcg aggcgaccaa tggacccgac gtacccatca cccggtctgc
      601 acggtcgtgc aaccgtaggg catgagtcga ctgtggtctt tacgagacaa tcgcttgtcg
      661 tgtccagcct tgcgacactt tcatcgaggt ttcactgttt ccaaaggacg tccgggctga
      721 ctgttcgtga cattggctac caacgaggct acaacacggt ctcgtgaacg acactattct
      781 gcgcaaaacc cccctgtacg aagaacacaa aaaacttgga gcccgcatgg tcccgttcgc
      841 cggcttcgag atgcccattc agtatgaggg actaacgcaa gagcaccacg cggttcgcaa
      901 cgccgtggga atattcgatg tatcccacat gggggagttg gtgctcacgg gcgagcatag
      961 cgagtccgtt ctcaattacc tcgtgacatc ggatgtaacc acgctcgtgg atggtcaggc
     1021 catgtatacg tgcgcctgca atgagaaagg gacgatcctc gacgatctac tggtatatcg
     1081 tcacggcaaa gaccgctgga tggtggtctg caacgcttcg aatcacgaca agatcgcctc
     1141 gcattttgcg aaagcagcca aagaccattg tggattcgaa gaccaatcgc ccgataccgc
     1201 attggtagcc gtacaaggac cgaagacgtt cgatgtcctt gcgcaattga gccctgaagg
     1261 ggcccgctgc tcggacttga aagcttttca cttcggctcc tacgaacttg ctgagattcc
     1321 ctgcatggtg gcgcggtccg gatataccgg cgaagatggc gtggagatat tctgtgccaa
     1381 tcgatacgcg cccaagctct ggtcgctgtt gctcgacaag gggcaagcct ttggcctcaa
     1441 accggctggc ctcggatgcc gtgataccct ccgcctcgaa gcgcgcttgt cgctgtacgg
     1501 aaacgatatc gatgaaacca ccaatcccct cgaagcgggc cttggatgga tcgtcaagct
     1561 cgacggcgac gacttcattg gtcgcgatgc gctggtcgaa atcaaacgac aagggctgac
     1621 tcgtaagctc gtcggcttcg aaatggtggg acgtggtatc gcgcgccatg gatacccgct
     1681 gcttgacctt gacgggaata ccgtcggggt ttgcaccagc ggttccccct ctcccaccct
     1741 cggaaaaaac atcggccttg gataccttct ttcctcgatg accaaggtcg ggacacgttt
     1801 caaggtcgac tgccgcggca aactcatcga agccgtcgta gtcaagaccc cgttctacaa
     1861 acgagccggc tgacctaccc cgcagcaggc gaggagtaat catgagcacg tttccgaatg
     1921 atcgtcgtta tacggtggac cacgaatggg caaaacaaga aggcggcagc gtcgtcattg
     1981 gcgtcagctc ttttgctgtc gaacaactcg gtgacattac cctcgtgagc atcgatgcaa
     2041 gcgtgggtga tcatctcgaa gcgggcaaag cgttcggaac catcgagtcc gtcaaaacgc
     2101 tttccgatct gctcgcgccc atttccggga ttctcgaagc cgtcaatggc gaactcgaag
     2161 actcaccgga gacgatcaat gacgattgct acggcaaggg atggatgatc aagatcaagc
     2221 cagcctcttc tgccgacctg gacaaactca tggatgatgc ggcctacgaa acgcatgtca
     2281 agagttcccc ccactaaccc cttgccagca tcgagccgtc gcatgcgcta cctgccccat
     2341 acgcagcaag agatcgaaca aatgcttgca gccatcggtg ttcccaccgt ggatgccttg
     2401 ttcgatacca tccccgccga atcacgtctt ggaaggcctc tcgctctcga gccaggactc
     2461 gacgaggtgt ctttgatgag acacctatcg gatctcgccg caggcaacca gggcgcaacc
     2521 atgctctctt ttcagggagc cgggatctat gatcatcaca tcccccccgc tgtcgatcag
     2581 cttctgctcc gaagcgaact gtataccgcc tacacccctt accaacccga ggtttcgcaa
     2641 ggaaccttgc aatcgatctg ggaattccag accatcgtct cggaaatcct cgggcatcct
     2701 gtcgccaatg cttcgatgta cgacggtgcg agcgcggctg ccgaagccgc tctgatggcc
     2761 cgacgtatca ccaaacgctc gaagatcatc gtttgcgatg gggtacatcc ggaatacatc
     2821 gaaacgatcg agacgtatat gtctggtctc gagggcggac gggctcatgt cattcgcaca
     2881 cgcgtgggcg atgagggcgt gacgagcctt gcgagcctgg gagaccgaat cgatgaccaa
     2941 gttgccgccg tagtcgttgg atatccgaac ttttttggat gcgtaaccga cctgcggccc
     3001 cttgctgaga gaattcatgc cgctggggcc ttgctcgtta cggttaccca agatccctac
     3061 gcgctggctt tgatcgaacc tcccggccat ctcggcgccg atatctccat tgcagaagga
     3121 caggccatcg caacgtctcc cagcttcgga ggtcctggtg ttggcctgtt cgcatcgcga
     3181 atcgaccgga agtaccttca ggaaatgccc ggacgcctcg ttggtgaaac cgtcgatgcc
     3241 aacggcaaac gaggctatgt gttgaccctc gccacacgcg agcaacatat ccgtcgagaa
     3301 cgggcaacga gcaatatctg cacgaaccaa gggctcgtgg cgctttccct cacgatccgt
     3361 atgtgcttgc tcggaaagca aggttttgtg gacgtggcgc gacaatgctt ggccaaagcc
     3421 aagtacctgc gtgaacagat cgcgagcctc gaaggctatt ccatcgtcta tccgaatacg
     3481 gctagcttcc acgagtttac catccgggtt cccggtcaag cgaccacagt cgtggaacgt
     3541 cttgcccgtg atgggatcct ggcaggagtg ccactttcca gatatttgcc gtcccgggat
     3601 gatcagcttt tggtagccgt cacggaaaag catacgcgac aggatttgga tcgcctggta
     3661 tccgcgttgc gagcgatctg aaactactcg agttttcgag gatcaaaacg ttttccctca
     3721 ccatcgaagc cataatgctt ccaggcatcc tcgggagtca aaaatgggtc tgccttgggt
     3781 gatgtgaggt ttcgaactac caaccgtgtt cctcggtcga ctttttcgac ctcgatacgg
     3841 agcaaaggag ccgtgctgcc tttgacgagc gcaccttcga aaatcgtttt gactgaaccg
     3901 atttccaccg tttttgaaac gacattttgc cgaaggtaat tggcaacccg ttcgggttgt
     3961 accgtgcctt cgcaatgcac gctaagcggc tcgcgtcgga tgattcgcat ggctttgggc
     4021 aggagcaagc caaaagctgt ttctttgccg ggggaaagct ctcctggggc aagccggtcg
     4081 ggtcgaggtt tgtcttcgac agatgagctg gtggatggtg tgggagttgg tgggtggtct
     4141 tgcttgcatc ctgtccagac aacgaggctg aagcaaacga gtgccaggtt cctgcgacaa
     4201 gaatggctac ggaaaaacat gcgatagggg tatcaatcga ggattgcggc ccaggagtca
     4261 atgacggacc gagcggaggg gggcaataag tcgaaggtat ccacgcggac ctttttatct
     4321 cctcctcctt ggttggctcc cacctgcacg acaagctgga aagcgcctgg attgaggctt
     4381 ttgatggttt ggtgtccagt catgcccagg aaaggatcct cacttaggtc atcctcgatt
     4441 gttatacagc ttggtttctg catgacccct acgccgaagg cgatacccgc ttcgttgaag
     4501 ctaatgacac gtagattgtt agccggggtg ctggacgtgg gatccggaaa ttgcggtaag
     4561 ggtccgtcca cggcggtagt gggtcctgaa gcgcctactg tctgtcccgc atgaagataa
     4621 tcgactcccc atagataact gcttcctggg gagcaggagc caggatcgat ggtttgtggg
     4681 ttgaacgatg taaagtaaag gaccgacgag aaaagagcca tggggcccgt gacccgctcg
     4741 ccggaaccgg cagcaaaatg cacggttttg tcactggtgc tcgtcgtatt tttggcattg
     4801 cttgcgttga gtatccaatt cacatccatt tggatgtcgc ctgttttaag attcttttgc
     4861 tccgtcaagg accataccga gtttctgccg accggggtta gagaggtttg ctcgcctgtc
     4921 gacagggcga tggtcacgcg gcccatgggg tcggtggaca gaatgggcgg agtttcgatc
     4981 ggtcccacat ccgccagacc gaacggacct ccccatgtcg tatccgtcgg gaaagcatcg
     5041 tggaccaagg ttacggacca gtcctgcggc tccgcagagg atagatcgag tcgccacaat
     5101 cggccttccg catcgccgat ataagctcgg tctgcgacag agccgggcgt attgggatag
     5161 gagatgatat ggccgaccaa gggggccgca aaaggcgctt tggttaccct gctattgccc
     5221 gaattcgttc cgtacagggc ttgcggcgcc tccggcgcgg ctgcattgta ggaatcactc
     5281 cgtcgaaacg ttcgaatgac ttctcccgta tcgagacgga cgatggtcag agaccgggca
     5341 cctgccagag agagcacatc ttgcgggtta ttactgacag gtgtgtattt agcggttttg
     5401 gttcgatggg tgaccccaac aatgttggcc accgatccag tggggaaagt cgtggtttcg
     5461 aggttggttt cgtcattgga acggactcct ccatatccac cgggcaggat agctacagcg
     5521 tgtcttgccg gaagggttcc cacaccgctt cccggcatga caaagtagag cgtcgcgatg
     5581 gcagggcgcg tggaacgtga tccaaacagc ggattgccgt ccttatccgt ggtgagctgc
     5641 cacaaaaacc gtggtccaga agtgtaaccc gtgacttttt tagtttcatc acgatccggg
     5701 ttggtcacgt ccaacgcaaa gtatccttgg gatgacccat aggttcccaa caaaaccgta
     5761 taccatttcg cggtgttgaa ggaagaaggg gtgggggccg tgcgcgtaag gaatcggccg
     5821 gcagtggggc ttgcatcccc agctacctcc cccacgacaa tcgggccgct caacaaggtc
     5881 ttgttggccg cttcataacg cgttgggtac tgttcgccaa tcgatgggag taccgcgggt
     5941 ggaatgaaac tccaaagttc gttgagcgaa ttgtcctgtt tgttgatggc aaaggcatga
     6001 agctggccat cggcggtagc cgcataaatc gtgggtactc gtttcgaaaa caggccttgg
     6061 aaaagcgtgt agctttcgtc acgaaggttc tcactcggcg gcatcacgag ttcgggagtc
     6121 gaattgatca tcgcgccaaa cgcggaggag cgaagataat caatgccggt cgccgataaa
     6181 ccaagctgaa gcttcaagaa tcggtcgcga cacgccgtcg ccgtgggaat cgtgttggcc
     6241 gacgtacgag cacaacaaag ctggcctgct gaatcgcatt ggttggttcc gaaaagcgcc
     6301 gtgggagtaa cctttgaggc gaagacgctg gcactatcct cgaccgtggt gccgcccacg
     6361 ctgcccaaac cgtccgggtc ggcgctcggg ggcagcatgg gacgtatgga tcgagtcgaa
     6421 tggatggtgc cacccgaata ggcatcgcca aggacggtgt agatatagcg agaactcaga
     6481 ttgccgtcca catttgcttc gaaataatcg cctttgctct tgtcgatctc ttgctcttcg
     6541 ggaatgttgg attcacacgt atatcgttct cgtacgagat gcccggcgta gaggtctttc
     6601 gagacgggat cgaaggagct gaagaatctg gcgcctacta tcgtatcgga aattttggtg
     6661 gccgaaatat ttccggtggc gaacaccggc tgggttcgcg tcatggccgc gggcggaaaa
     6721 cgcaggatat tggatattgc cgcaagcagt tccgttcccg actcggccca ataggcgctt
     6781 cgaggggctc cgagcgtcat tcctttatct tctgccgtat acctgctgcc ctcgttggca
     6841 attccttcga tggtgcagca ggtggcaagt aattcatcct ccggggtcga tatgcaataa
     6901 ccataagcgc attggtttgc ggcacacgtg ctggtacagg gtggcaaaga actacaggcg
     6961 cggtattccg gatctttgat gttcatcaat tgactacagg tcaatgcgct gctgccttta
     7021 gccgctgtga ccgcaaatcc gaccacgaag agatcgatat ttttatcgta tagatctttg
     7081 gcgtagcttt gtggtgtctc gaaggggcac ttcccatcat tggtggatgg ggtggctttc
     7141 aagtcgcaat ggggacgcaa gtcgaggttg ggcccgccat ccgtgatcaa aatcgccatt
     7201 ctcttgcgac aacccggatg agtccgatcc atggatacat tggggtccac cgcacctccg
     7261 tagaagctgg ctccaccaat atccaccggt ttcgtattgt cttgcgtcaa aaaaaagagc
     7321 gcatctttga gcattccagc cacaggtgtt cccccaaagg gacgtattcc caaaatcgct
     7381 tgctgcaccc tttcattgtg ggcaatgatc tcctcggtgg tggcattggg gtcgccaaaa
     7441 ccaatgaggc gtccttccca gggaggcgcg gcggggttgc gcgcccccac ctcgaacaat
     7501 cgagccgctc ccggaggagg cgggcagtcc acaggccatc cttgccccgc atactgcgtg
     7561 tctgtcgtca accaacctgg aaaataactc cacgtatcac cgactccaga ggcaaaatca
     7621 ggcagaaatg tggaaagtat tcctgttcca ataccacctg tgtccacacg agacggggaa
     7681 gggtcgaagg tcatgatgcc gaatcgaacc gaagtttgaa ggcggtcgat gacaccatcc
     7741 gattcttgag gcataaaagg acactcgact cccgatgaat acgtttgcga catatctcgc
     7801 cagtagacag gcgccttgtc cggccatttc atcgcgttcc cattggtcag tgcagttcga
     7861 agatcagtgt cccaagaagg ggcaggcgta caacgctgct tcgtgccatt gaccgtatac
     7921 gtagcgacga ttcggttgaa cggaagatag tatcctttgt cgtaggggtc cgccgcaccg
     7981 agcttgtatt caccggcgaa atcagtgttc gagcgctcct gggcggagca ttcggcgccg
     8041 atgacagtgc ctgtaaacgc attggcgagg atcgcccatc gatccgcggg ggttggtccc
     8101 gatgaaaaag acgccgcgga atcgaaagac attgggctcg aggccgggta gactccacat
     8161 tgcggaagcg catagcgagt cgtatcggtg gtcgaacgct tcgtcgtata ggccatcgaa
     8221 ccggatgtat cgagaagcag aaggacgtcc gcggcctggt actccgcgga ttgtgccttc
     8281 gccggatgac cgaccgtttg gagcgtcacg accgccgcca gagaagccag aattcggagt
     8341 ttggtatgcg tcatggctcg aatgctcctc gaatcaacat ctttcatcgc ggcaaaggac
     8401 ccacttgtac gatggcccgg ttagcgcgaa tcgccgccaa acgaaccgat tgctggttgg
     8461 tatcgtcggg acgaaccacg cccgtgcccg aaaatgtcag ttgcatatag cggaatcgtg
     8521 gaccgatgcc ggattgatcc atacccgcca ccggtcggcc cacggggccc ggatcggtca
     8581 gttccaccaa aaaatcacct cgtaaaagcg ccatcggaga actcaggctc gtgcccgatg
     8641 aatcgatgag gtagtcggct ccgctttccg ttttcatcct gttttcgata tcggttttcg
     8701 tgagtcggta gcatgggagt gctgagatcc cagtaatata ctggttggca cggcacgatt
     8761 cattccccgc catcaccaat tgcatgtatg ccagcgcctt acccccagac aattcgtcca
     8821 caaccgctac cgttccgaga tcggctatat acgcggattg ggtcgattgc ttggcatacc
     8881 ctgaaatgcg ctgattcaaa cctgccgtat gggcagcgaa tatcccgacc gctgtcaaga
     8941 ccgtgatgac ggacatgacg acgaaaatcg aagcaccccg ctgttggcgt cgtcgcttga
     9001 aaaccgccag tgaagcaggg ggaaaacagc ggcctgcgga ttggcttacc atgcctcacc
     9061 tcggtgattc atcagcgaaa tcgtggccgt caacgtacgt gttcgagccc atcggccttc
     9121 cgacactttc gagcgaaaca tatagccatc cgaaacgagg ggattggcca cttcatcaat
     9181 ggtttcggaa cggtcagcct cccgtgaacg cacgacaagc cgaagatcaa gcgcccggat
     9241 agccgtgggt ccaccgacag gagcaagggc aacggttggt agggtcgctc ccgctgccga
     9301 acccggagca aggtacctca gatttcctcg cacgactcct ccggatgccg tactggtggt
     9361 ccagactccg actttcaaat ctacagcaaa ttctgcgaca atttcaggct gctcggtgag
     9421 ggaaggcaaa gtgctgccag aaccgccagc gccacccgcg ccacccgcgc caccagaccc
     9481 agagccactg ctctccaaca acaacacctc gctgcgtacg agttccatcc gattcgcgtc
     9541 cgaatccgcc acacggtaat tctcgtgata gatcgtgtcc ttataaggac tgagggccga
     9601 tagattcgct atccgatatt caacgacact gattgggttg atttgggtgc caagtccgtc
     9661 cgaaagaccg caggaagccg aagtcgctcc cgatgccatg gaaatttccg agcgcagtgg
     9721 gagccgcccc accgtcgtga tcacgggaga cgccgaatag ctagaggatt tgatgatttc
     9781 gtactcatat ttcccctgat cgtcgagaat acgaatcaac cggcccggac ggaacaccgc
     9841 ttgaaacgcc gcatccctat ctgaagcgct cggtatgaaa cccgctcggg tcatgggtcc
     9901 gttattcgtt tggagaaata cctcgtggcc cgtccctttt gcggcaatcg ccagtacagg
     9961 aaacatttct gaagaatcca ggttccctgc caccctcacg acatccggtt tgctggttgc
    10021 cgaagatatt ctgatacttg tgagttcttt gacgctgcct ggccatgtgt cgtagtttgg
    10081 agcacagatt ttattggccg cgtagtcacg tgccatatcc gttggggcca tgtacgcgga
    10141 ccgagccaaa tccgcttgaa gacgttgaaa gccagcgagt acggacattt gggcgcttgc
    10201 gatacgcgat tcctgcgcaa aaaaccgggt agcttgccgg gaaaaggaaa ccgccgccgt
    10261 cgctaccaga agccccgcca tgatggccac gagcagctca atcagtgtaa atccccgctt
    10321 gctcgtttgg cgactatcgc tctgccggga catccttcga accctcatgg cgcctcgttt
    10381 ttcgtcacca tcgtgacggc atgagccacc cgaaaaatac ctttggctgc tagatcatcc
    10441 accgtggccg ggtctccaca cggcaagtct ttctggtcca ccgcaccgtc cccctcgttg
    10501 cgacctaccc aatggacccg tacttccgcc cggatcatcc gttggtcctc ccgcacccaa
    10561 ctcagccgaa gattgacaca ataaggacct tgaaccgttg cccctcccga acacggttca
    10621 tccctgccaa acacatcgtg cgtgccgcaa atgtcatttc cattcgttgg ccggaaccaa
    10681 tccgtcttcg tgggatcagg gcctttgatg tgatttttca gccacaaggt atccacattg
    10741 agatccgatg ttggattggt gggcgatggg tagttccata acatggcgtc cattctcaat
    10801 cgctcgatcc acgtgcgcgc gacttcgttc gccaccgcaa tggttctcga gtttcgattc
    10861 gccgacgacg ttaccttctg caacgccacc accccggagg caccaatcgc aagcacggta
    10921 atcgccatca tcacttcgat gaccgtcgat ccacgccggt tatttcgcct cgcgttcatc
    10981 agctctcctt caccttcgcg gcgatgcgcg ccatcccgtt cggcaggatg aataccaccc
    11041 ggcttattcc cacctccgtc gttccagaca tctgccccac acgaatcatc aaggtccgcg
    11101 gcgacgagct aaacgccgtt gttgttccct ttcggaacag catcgtaccg ctaggtgtga
    11161 aacaaacatc gccaacctca ctgtctccaa tgacggttgc cgtcgctcca gattgcgcgg
    11221 agtaaattga aaactccgtt ttgacgaggt cgtaaggcgc ttgtccgccc aacgtaaatg
    11281 attcgatctc ttcgaataag tctgaagaat catcccaacg agtcgccggc atggtacagc
    11341 ttgcgaccgg aaaataatta gtcgtgctac cgcctcccgc cgctccccct gccccaccgc
    11401 cggaagaact cgcggagctt tttcccacca cagcttcacg aaccttgaac tcaccattgg
    11461 aatatcggac cag
//
LOCUS       DAOSOR010000125        11437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5649_length_11437_cov_4.400808, whole genome shotgun sequence.
ACCESSION   DAOSOR010000125 DAOSOR010000000
VERSION     DAOSOR010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11437
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5649_length_11437_cov_4.400808"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PK710_08935"
     CDS             <1..691
                     /locus_tag="PK710_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09882.1"
                     /translation="IAQACQKQKMACDMAMRQHRYFFPRLAVLLDAFSLLRPATRAHE
                     VASLRLRLMAEASPRQVDPQERRLALRLRDMRTQMIGLIGDVRACRSCARGYPLPHGR
                     WEGGYCCGGTTENVFQQEELACLRASGTRPRDFRTPRAVHAGCAFRGPRGCSLAPAHR
                     PNLCVRYTCRDLHEEFSKRGIERQVRQLASQIQRTFSEYRSLRSNRLDRESLERFEAE
                     TKKISGKRMNS"
     gene            complement(714..1835)
                     /gene="hisC"
                     /locus_tag="PK710_08940"
     CDS             complement(714..1835)
                     /gene="hisC"
                     /locus_tag="PK710_08940"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOT09883.1"
                     /translation="MAKKILLRTPLRAFKPYVAGKPIEEVRREYGLTGHIAKMASNEN
                     PLGTSPKALEAMRKAIEEVYLYPDDNAFYIRKKIAQTHQVAFENVFAANGSVEVLELA
                     VQAFVEPGDGVVTSEHTFAIYMLAAMKIDAQLRMAPMIDGGYRYDLPAIGKLVDDRTK
                     IVFLANPTNPTGTWFSGEEFDTFMAHVPEDVLVVYDSAYAEYCPYPDMPDPMKHYRQG
                     RRILCLRTFSKAYGMAGIRVGYAIGPADIIHGLMTCRVAFNLNLVAQAGAMAALDDDE
                     FVRRSREFNDRELAFLREGMKELDVIVPPSRANFLLVDTKKNAQWLFEELQKQGIIVR
                     PMGGYGYPGAIRVNPWQREDNERFLSSLKRLLASDQGSL"
     gene            2021..3100
                     /locus_tag="PK710_08945"
     CDS             2021..3100
                     /locus_tag="PK710_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09884.1"
                     /translation="MNVRWPQLICMACAIAQAGCSEDSDHDRNANGGQAGASGATQSI
                     ACESTTSPQLEGTWAVLARYSLQLQSRQGGVVSMCPVDQVAPATLLFILDIQSSAQAA
                     LDVSAVPCQLELPNVSAMVGECRPEQSNGLNVTIPIPEPLTRGFATVPPTQIQGSISP
                     SDALSFESLGFTWGTRSPTLPEWNASASGCGMSDLDIGRSETCEPSCVDACDALVDDD
                     QDGFPGVTVHVCGTTEDDVRTQVPCHAEQPTIPGVTLQGMIRMALRTQLVLQGQALSS
                     CEALGTFASQTEYAVVGADTYLTNVRVSVASARQSLPLFKGKTEDSRWRMIRVDGRHG
                     AADWNLPQDAASRCSIVRSRRNELE"
     gene            3320..4945
                     /locus_tag="PK710_08950"
     CDS             3320..4945
                     /locus_tag="PK710_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09885.1"
                     /translation="MPSASRAAIHSNVETHEAHAEVIAWARRQSLRPLRLLASVCLMV
                     STAVAIGWISQRMARPTRFDEDLALAKAPMKPEPWLPCFDPNAIESIDFEAVHAHLLP
                     EWVIALHHDPYSVGSSREQRRFDALIAEAGKDPNLALLLRRLRDRATLGVSLHALEIS
                     QLLDGWNHTMQQAGLPWYVAYDSVESARGGRLYTRCYHVQADVLVPVGPQTHGVRLLS
                     RVDSTNVAEAFFGQTSTEEQRALVVGDRVAEFALERLWPMMEPQLDAKCSEMDRAFAP
                     ALRHEASQWLSDDTLESLRQGAMLRHQLIERLQEIERKKGCGRWLAVDHVPWNGLSEK
                     GRSIIARAAQVNERKRCDRLTHKDAQWLIGVSEALSSDEKLKDSLGQLAAWLARAVAS
                     HEVRHLADGVPGRVLTCDACPEGMSERAKAEVSAYVASFASKRTAFVALYQACGVDVP
                     VSHGSGDALRFVLGVLMPQGCESPPPNNLVEQARLLDWILFKRVQPIEITDTFPKSVP
                     LPKERTPPSLGFETVAQGQKRHSLRPAFPMRSL"
     gene            5011..5664
                     /locus_tag="PK710_08955"
     CDS             5011..5664
                     /locus_tag="PK710_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine phosphatase family protein"
                     /protein_id="HOT09886.1"
                     /translation="MNNASDLQPSEEQIRELAMWLKAMPELADLAQRQPGDPERPRLF
                     YAKLRDKPVYPMVHIPVMGRAGTLWVGRIPGAGLLDMTRELDAIMAFGITRIVCLAPL
                     DQSSAYAAAARERLSDGFINLPVADFGTPADDRRFEREVLRTDEALHKEQSVLVHCLA
                     GCGRTGMFVSCLLVRVGAAPQSAIRGFRRLRGCGPETPEQVAYVVRYARRLGENSDD"
     gene            complement(5661..6974)
                     /locus_tag="PK710_08960"
     CDS             complement(5661..6974)
                     /locus_tag="PK710_08960"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HOT09887.1"
                     /translation="MRHTPIPTPPSGAFEATMEDSKHPDFIKQFYLDCGFSTRALHAG
                     EHVGQPHTPAHNTPIYQSSTFIFENADHGAAIFKGETPGYVYTRMGNPTVMVLEAKIN
                     ALEGGSWKLQHPEDTISTLAFSSGMAAIAAALMACASTGDTIIMADVVYGATEHLCST
                     VLKRYGIKTVEVHMADLDAVRATVKAHPNAKLIFFETPMNPTLELVDIPAVVEIVRQT
                     NPDMRVIVDNTFATPYLQRPLEMGADVVVHSTTKYIGGHGAVVGGLATTRHNTIKDEL
                     YKVIKDIGSNPSPFDTWLVNMGLKTLPIRMDKHCKNAMAVARFLESHPKVERVFYPGL
                     ESHPQHALAKRQMADFGGMLSFELKGGFEAGKKLMDTIQIFTLAVSLGCVDSLIQHPA
                     SMTHASVPKEKKLKGGITDGLVRISVGIEDPDDLIRALDKALQKV"
     gene            7465..9285
                     /locus_tag="PK710_08965"
     CDS             7465..9285
                     /locus_tag="PK710_08965"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT09888.1"
                     /translation="MSLVSVESVPSSIGKYRLLAELGHGGMARVFLAVASGPSGFSKL
                     VVLKMIRVHLAEDPDTLQMFLDEARLAGRLNHPNVVHTLEVVEEDGRYLMVMEYLEGQ
                     AFASVLRRARKENMPMPLPLYLRGLCDALQGLHYAHELKDFDGTPLNLVHRDVSPQNI
                     FITYDGQVKMLDFGIAKAITSSSETKAGVIKGKIAYMAPEQFLGEKLDRRVDIYAMGA
                     VLWQAATGQRLWKGVPDGQVIHRVIRGEVPEPMSVNPGVHPELARIVMKALARDRADR
                     YATCLELQSDLDALIDSLGEKVSGRDVGNFVGDMFVDVRKEIAATIEAQLSKAQNYDT
                     QAFDRTVIPAIQSMTNPGSLLSMDSSGQHLRSLSGMKMLSPGHTDSEIISASVRSNAI
                     LSNSVSGAHPVGGRKSGLLIAVVAALFVGIAGTVWLVPSVRSAISGEQTPATPSDAPA
                     TAVAQPSTPQPAQSTESTAKEPAATQAEIEIRAEPANARIFFEGAELATNPATRRVPK
                     DGKVYKIRVEAKGFESREVEVTGEKDVVVTVALDKSRAIGGPAVAPPRVTKEDPKGHK
                     PPETAPPPTSSPPPPKSTKTQEPNARDRIRNLDKSNPWEQ"
     gene            9291..10268
                     /locus_tag="PK710_08970"
     CDS             9291..10268
                     /locus_tag="PK710_08970"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOT09889.1"
                     /translation="MHYRKIVAMGIVLGAMASPGFVSAQSVGTPAAQQEAMQRFQRAV
                     DLYEEQDYAAALTEFRKAYELAPSYKLLYQIGQVSYKLKDYAGALQAFEKYLSEGKDE
                     ITPERRAEVQEEINLLKNQTGHLEINSNVFGAAVTIDDNPVGTIPLDGPILVSVGSRK
                     ITVSAEGRQSVTQAITVAGQELRKLNFDLPELVGETRTVYQKESPSKMTTWSWVGVGV
                     AGALGIGATITGIMAMNADEDLSNMTFVGGTPDSKIKDQQSKVDNLALTTDILAGAAI
                     VTLGTTLVWTFARPDAPPEFDSEPPPAEAKRPFIQPMIGLGSIGVTGEF"
     gene            10270..>11437
                     /locus_tag="PK710_08975"
     CDS             10270..>11437
                     /locus_tag="PK710_08975"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT09890.1"
                     /translation="MRRTNLLRTLATTFLSVGFAVAAASSCSLITSSDIAPGGIGQPC
                     KDDSDCQGASCDNGLCTAACSTTAQCPEPSQCLNNTCQLPLRVAGIWIGLATAAEGWT
                     LTHQEGIEFAKEKLGYIDYVYEEGVLGEPAEKAIDKYVADGYQVIIANSWDHLATVRK
                     KAAQYPNTTFLHCSGIPVEPNVSSFFAHMEHAWYVAGRIAARKSKTGHLGFIGSFVTA
                     EVVRHLNAFALGAKKENPNAKVEVRWLGFWYDVDFDKALWEYTPKHMGDKAVKKNMTG
                     EEYLTAKLIDSGADVITHNLDNQLPSRYVGIHTTAKTMKNPKNPDQDWVVWTIANDNI
                     HGWRDQSGQPFANAIGATYWNWGYPYIEIFEDIHRGRLTSKAYMYPLTTDTETSV"
BASE COUNT         2511 a         3150 c         3295 g         2481 t
ORIGIN      
        1 aatcgcccag gcgtgccaaa agcagaaaat ggcttgtgat atggcaatga ggcagcatcg
       61 ctatttcttc ccgcggctcg ctgtcctgct ggatgccttc tcgctgcttc ggcccgcgac
      121 tcgcgcccat gaagttgcca gccttcgact tcgtctcatg gcggaagctt ctcctcgaca
      181 agtcgacccg caggaacgcc gtctggccct taggcttcga gacatgcgaa cgcagatgat
      241 tgggctcatc ggcgacgttc gagcttgtcg gagttgcgcg cgagggtatc cgctccctca
      301 tggtcgttgg gagggaggat actgctgcgg aggaacgacg gaaaacgtgt ttcagcagga
      361 agaattggct tgtttgcgag cctctggaac gcgcccacga gattttcgaa ctccgcgcgc
      421 ggttcatgcg ggatgcgcat ttcgtggtcc acggggatgt tcccttgccc cggctcatcg
      481 tcccaacctt tgtgttcgat acacatgccg cgatttgcac gaagaatttt caaaacgagg
      541 gatcgagcgg caggtgcgac agctcgcttc gcagattcag cgcacgtttt ccgagtatcg
      601 gtccctgcgg tcgaatcggc tcgaccgcga atctctcgaa aggttcgagg ctgaaacgaa
      661 aaaaatcagt ggaaaacgaa tgaattcgtg agatccgaag tgtccgtaaa cggttagaga
      721 gagccttgat cggaggccag caatcgtttc aaagacgaca ggaatcgttc gttgtcttct
      781 cgctgccagg ggttgacgcg aatggcgccg ggatagccgt agcctcccat gggccttacg
      841 atgattccct gtttttgaag ctcttcaaat agccattggg cgtttttctt cgtatccacg
      901 agcaagaaat tggctcgcga cggagggacg atcacgtcga gttctttcat tccttctcga
      961 agaaatgcaa gctcacggtc gttgaattct cgcgatcgtc gaacgaactc atcgtcgtcg
     1021 agcgccgcca tggcacccgc ttgcgccacc aagttgaggt tgaaggccac ccggcacgtc
     1081 atcagcccgt gaatgatatc cgcgggaccg atggcgtatc caacccgaat ccccgccatt
     1141 ccgtaggctt tcgagaaggt acggagacac aggattcgac gtccctgccg gtagtgcttc
     1201 atgggatccg gcatatccgg atatggacag tattccgcgt aagcgctgtc gtataccacc
     1261 aacacgtctt ccgggacatg agccatgaac gtatcgaact cctctccgga aaaccacgtg
     1321 cccgtcggat tcgttggatt cgccaaaaat acaatcttgg tccgatcgtc gaccagcttg
     1381 cctatcgccg gaagatcgta tcgataccca ccgtcgatca tgggcgccat acgaagctgg
     1441 gcatcgatct tcatggccgc gagcatgtag atggcaaacg tatgctctga cgtcactacc
     1501 ccatcgccag gttcaacgaa ggcttgcacc gcgagttcga gcacttccac cgagccgttc
     1561 gccgcaaaga cattctcgaa ggctacctga tgcgtctgtg cgatcttttt tcggatgtaa
     1621 aacgcgttgt catcgggata aaggtatact tcctcgatcg ctttgcgcat ggcctcgagg
     1681 gctttgggag acgtgcccaa ggggttttcg ttcgatgcca tcttggcaat atggcctgtc
     1741 aagccgtact cgcgacgtac ttcctcgatg ggtttgcctg cgacatacgg tttgaaggcc
     1801 cgaaggggag tgcgaagcag aatcttcttt gccatgccgc catcctagcc atcgatgcgc
     1861 cgcgtttcca ccaaaggctt tcgaatatcg tcaggaaaga acgtgtcacg gaagaagtca
     1921 ccgccttccc accattgacc ctacagaacc ctcgacgtac gactccatct cgaagatgtg
     1981 gatcgcccaa caacccctgc gctggaagaa agtctcacga atgaacgtac gatggcccca
     2041 actcatctgc atggcctgtg ctatcgccca agccgggtgt tcagaggatt ccgatcatga
     2101 tcgcaatgcg aacggcggcc aggcaggggc atccggtgca acacaaagca tcgcctgtga
     2161 atccacgacc tccccacaac tcgaggggac ctgggccgtt ctcgcacgct attcgctcca
     2221 actccaaagc agacaaggtg gcgtcgtgtc gatgtgcccc gtcgatcaag tcgcaccggc
     2281 taccttgctt ttcatccttg atattcaatc ctccgcacaa gcagccctcg atgtttccgc
     2341 cgtgccctgc caactcgaat tgcccaatgt ctccgccatg gtcggtgaat gccgtcccga
     2401 acaaagcaac ggcctcaatg tcaccattcc cattcctgaa ccgctgactc gtggctttgc
     2461 caccgtccca cccacccaaa tccaaggctc catttctcct tccgatgccc tttctttcga
     2521 aagcttggga tttacgtggg gaacgcgaag ccccacactg cccgaatgga acgcatcggc
     2581 atccggttgc ggcatgagcg acctcgacat cggtcgatcc gaaacctgtg agccctcttg
     2641 tgtcgacgct tgcgatgctc ttgtcgacga cgatcaggat gggtttcccg gcgtgacagt
     2701 tcatgtctgt ggcaccacgg aagacgacgt gcgaacgcaa gtcccctgtc atgccgagca
     2761 accaacgata cccggtgtca ccctgcaagg catgatccga atggcccttc gaacacaact
     2821 cgttctccaa ggccaagccc tttcctcctg cgaagccttg ggaaccttcg cgtcgcagac
     2881 cgaatacgct gtggtcggtg ccgataccta cctgaccaat gttcgggtca gcgtcgccag
     2941 cgccaggcaa tccttgcccc tattcaaagg aaaaacggag gatagccgtt ggcgcatgat
     3001 ccgtgtcgac ggtcgccatg gggctgcaga ttggaatctc cctcaagatg ccgcttcacg
     3061 ctgctctatc gtccgctccc gtcgcaacga actcgaatga cgctacgtca cgatggctcg
     3121 tagcaacgcg gaatctgcga aatcatccgt atctcgcatg ccgccctgac tccgtctgaa
     3181 tatcggtgcc cgaggcagaa cggctttttt caaggatgag tggaagattg cgcggccata
     3241 gaaagccttc atcccgggga acaaatgccg tgcaacaggg tctgtgtttg gtcgcgcacc
     3301 cctgcaggag gtccaccgaa tgccgtcagc atctcgcgct gcgattcatt ccaacgtgga
     3361 aacccatgag gctcatgcgg aagtcatcgc ctgggctcgt cgccaatcgc tgcgaccgct
     3421 gcgtctgttg gcatcggttt gtctgatggt atccacagcc gtggcgatcg gctggatttc
     3481 gcaacgtatg gcacgaccca ccaggttcga cgaagacttg gcgctagcca aggctccgat
     3541 gaaaccagag ccctggttgc cttgtttcga tcccaacgcc atcgaatcca tcgatttcga
     3601 ggctgttcat gcccatcttc tgcccgagtg ggtcattgcc ttgcaccatg atccctacag
     3661 tgtaggaagc agtcgcgaac agcgtcgctt cgatgccttg atcgcggaag cgggcaagga
     3721 cccgaatctt gccctgttgt tgaggcgttt gagagatcga gccaccttgg gcgtgtcgtt
     3781 gcacgctctt gaaatctctc agttgttgga tggttggaac cacaccatgc aacaggcggg
     3841 gttgccctgg tacgtcgctt acgattcggt cgagtccgcg cgaggcggtc gtctttatac
     3901 ccgatgctac catgtgcaag ccgatgtgct cgttccggtg ggtccgcaaa cgcatggggt
     3961 ccgacttctc tctcgtgtgg atagcaccaa cgttgcggaa gctttttttg ggcaaaccag
     4021 caccgaagaa cagcgagcct tggtggtcgg tgatcgcgtg gccgagttcg ccctcgaacg
     4081 cctttggccc atgatggaac cccagctcga tgcgaaatgt tccgagatgg accgcgcttt
     4141 tgctcccgcc ctgcgccatg aagcaagcca atggctttct gacgacaccc tggaatcgtt
     4201 gcgtcaagga gccatgctac gacatcaatt gatcgagcgt ttgcaggaga tcgagcgcaa
     4261 aaaaggatgc ggccgatggt tagcggtcga tcacgtgccg tggaatggtc tgtccgaaaa
     4321 gggacgatcg atcattgctc gggcggccca ggtcaacgaa cgaaagcgct gtgaccggtt
     4381 gacgcataag gacgcccaat ggctcatcgg cgtgtccgaa gcgttgtcgt cggatgagaa
     4441 attgaaggat tctctcggac aattggcggc ctggctggcg cgagccgtgg cttcccatga
     4501 agtgcgtcat ctggccgatg gggttcctgg ccgcgtactc acatgcgatg cttgtccaga
     4561 agggatgtcc gaacgtgcaa aggccgaggt atccgcttac gtagcctcgt ttgcctcgaa
     4621 gcgcacggcg tttgtggcct tgtatcaagc gtgtggcgtg gatgtgccag tctcccatgg
     4681 ctccggtgat gcgttgcgat tcgtgcttgg cgtgttgatg ccgcaggggt gtgagtctcc
     4741 accaccgaat aacttggtcg agcaagcgcg gctgctggat tggatcttgt ttaaacgtgt
     4801 tcaacctatt gaaattacag atacttttcc caaatccgtc cccctgccca aggaacgaac
     4861 gccgccctcg ctcggattcg aaaccgtggc ccaaggacag aagcgccatt cgctccgacc
     4921 cgcctttccg atgcgttcgc tatgaagaca atcgacgccg cacgggcgtg acactggagt
     4981 tatcgaaggt cctggggtag gggagaggcc atgaacaacg cctcggacct tcaaccgtcg
     5041 gaagaacaga tccgtgaact tgcgatgtgg ttgaaggcga tgccggaact tgcggatctt
     5101 gcgcaaaggc agccgggcga cccggagcgt cccagactat tttatgccaa gctccgcgat
     5161 aagcccgtgt atccgatggt gcatattccc gtgatggggc gtgctggaac gctttgggtg
     5221 ggccgtattc cgggggccgg cttgctcgac atgacgcggg aattggacgc gatcatggcc
     5281 tttggaatta cgcggatcgt ctgcttggcg ccgctcgatc agtcgtcggc ctatgctgcg
     5341 gcggcgcggg aaaggctgtc ggatggtttc atcaatcttc cggtggcgga ctttggcaca
     5401 cccgcggatg atcgaaggtt cgagcgggag gtcctgcgaa cggacgaggc gttgcataag
     5461 gagcaatcgg tgctcgttca ctgccttgcg ggttgtggtc gaacgggaat gttcgtttct
     5521 tgtcttttgg tgcgggtggg tgcggcgcct cagtcggcca tccgaggatt tcgacggttg
     5581 cgggggtgtg gccctgaaac gccggagcaa gtggcgtacg tggttaggta tgcgcgacgg
     5641 ttgggggaaa acagcgacga ttagactttt tggagagctt tgtcgagcgc gcgaatcaga
     5701 tcgtcggggt cctcgattcc cacggagata cgaacgaggc catcggtaat gccgcccttg
     5761 agtttctttt ctttcggcac cgaagcgtgg gtcatggagg ccgggtgttg gatcagtgaa
     5821 tccacgcaac cgagtgatac ggcgagcgtg aagatttgaa tcgtatccat cagttttttg
     5881 cctgcttcga agccgccttt caattcgaag gaaagcatcc cgccgaagtc agccatctgt
     5941 ctttttgcca aagcatgctg gggatgggat tctagacccg ggtaaaacac acgctcgact
     6001 ttgggatggg actcgagaaa acgagcgacg gccatcgcat ttttacaatg cttgtccatg
     6061 cgaatcggca acgtcttgag ccccatattg acgagccagg tatcaaaggg gctcggattg
     6121 ctgccgatgt ctttgatcac cttgtatagc tcgtccttga tggtgttatg gcgcgtggtg
     6181 gccaaccccc ccacgacagc gccatgtcct ccgatgtatt tggtggtgga atgtacgacg
     6241 acgtctgctc ccatttcgag gggccgttgc aagtaagggg tggcgaacgt gttgtccacg
     6301 atgacgcgca tatcggggtt ggtttgacga acgatttcga cgacagcggg gatatcgacc
     6361 aactcgagcg tggggttcat cggcgtttcg aaaaagatga gcttcgcgtt gggatgggcc
     6421 tttaccgtcg ctcgtacggc atcgagatcg gccatatgta cttccaccgt tttgataccg
     6481 tatcgtttga gtacggtcga acacaggtgc tcggtggcgc catacacgac gtcagccatg
     6541 atgatggtat cgccggtgga ggcacaggcc atgagggcgg cggcgatggc ggccatgcca
     6601 gaggaaaagg ccagggtaga gatggtgtct tcgggatgct ggagcttcca ggagccgcct
     6661 tcgagagcgt tgattttggc ttcgagaacc atgaccgtcg ggttgcccat gcgcgtgtac
     6721 acgtagccag gggtctcacc tttgaagatg gcggcaccat ggtctgcatt ctcaaaaatg
     6781 aaagtcgatg attgatagat gggtgtatta tgggcgggcg tatggggttg accgacgtgt
     6841 tcaccggcgt gcagagcacg ggtagaaaag ccgcagtcga ggtagaattg tttaatgaag
     6901 tcagggtgtt ttgagtcttc catggttgcc tcgaaggcgc cgctgggcgg agttggaata
     6961 ggggtatgtc gcaacggtgg cagcctcgct cagccagggg ggccagacgt tgcggtgggc
     7021 ggtaccctag cacggggcgc gatggaggaa atcctaatta atcgttgcaa ttccgcaaca
     7081 tgggaaaggt gacgataacg tcagggggtg attcactatt tcctttcgca aactggggtc
     7141 gggtatgtca atcctggtgg acacgagcgg atcacaccat ctatagtgag ggagtgaccg
     7201 ttggcatgtg cgaacatctg gggcaatttc cgtgttgttg gcaacaattt gatgttgcgg
     7261 tagcgcttga agcacggact ttcgcgcgga ttccaagaat tgacgaagga gcgggaggcg
     7321 agtgaaaaac cattttggga tattgaagga tacatggatt ccgtgcttga acagatccgt
     7381 tggggcaggt atcgtatccg atgacctcgt gcgatggggg tattcgaggg ggagagaaaa
     7441 aaagcaacgg gagggtaatc aatcatgagc ctcgtgtctg tggaaagcgt tccctcctcg
     7501 atcggtaaat atcgattgct cgcggaactc ggccacggcg gtatggcgcg cgtctttctt
     7561 gccgtggcga gtggccccag cgggttcagc aagcttgtcg tgctcaagat gatccgggtc
     7621 catctggcgg aagacccgga tacacttcaa atgtttttgg acgaggcaag gctcgcgggg
     7681 aggctcaacc atcccaatgt ggtgcatacc ttggaggtag tggaggaaga cggtcgttac
     7741 ctgatggtga tggagtacct cgaggggcag gcgttcgcct cggtactgcg gcgtgctcgc
     7801 aaagaaaata tgccaatgcc tttaccgctg tatcttcggg ggctttgcga tgcgctccaa
     7861 ggacttcatt acgctcacga actcaaagac ttcgacggaa ccccgctcaa tctcgttcac
     7921 cgggacgtat caccgcagaa tattttcatc acgtacgacg gccaggtgaa gatgttggac
     7981 ttcgggattg ccaaggcgat cacgtcgtcc tcggaaacga aagctggggt catcaaaggc
     8041 aagattgcct atatggcccc tgaacagttt ttgggagaaa aactcgatcg tcgcgtggat
     8101 atttacgcga tgggggcggt attgtggcag gccgccacgg ggcagcggct gtggaaaggc
     8161 gtccccgatg gtcaagtgat ccatcgggtc attcgtggag aggttccgga gcccatgagc
     8221 gtgaatcccg gagtccaccc ggaattggcg cgaatcgtca tgaaagcgct tgccagggat
     8281 cgagccgatc gatatgccac atgcctcgaa ttacaaagtg atctcgatgc gctcatcgat
     8341 agccttggag aaaaggtctc agggcgagat gtgggcaatt tcgtaggcga tatgttcgtc
     8401 gacgtgcgaa aagagattgc ggctaccatc gaagctcagc tttccaaagc gcagaattac
     8461 gatacgcaag ccttcgatcg cacggtcata cccgccatcc aatcgatgac caatccgggc
     8521 agcttgctgt cgatggatag ctccgggcag cacctgcgat ctctttcggg gatgaagatg
     8581 ctctctccgg ggcatacgga tagcgagatc atcagtgctt cggtacgttc caacgccatc
     8641 ctttccaatt ccgtgtcggg agcgcatccg gtaggaggac gaaaaagcgg tttgctcatc
     8701 gctgtggtcg ctgcgctatt cgtggggatt gcagggacag tttggctcgt tccttccgtg
     8761 agatcggcca tttcgggaga acaaactccg gctacccctt cggacgcgcc cgcaacggcg
     8821 gtggctcagc catccacccc acagccagcc caatccacgg agtcaaccgc gaaggaaccc
     8881 gccgctacgc aagccgagat cgaaatccgt gcggagccag ccaacgcgag aatctttttc
     8941 gagggagcgg aactcgccac caatccagcg acccgtcgtg tgcccaagga tggaaaagtc
     9001 tataaaatac gtgtagaagc caagggcttc gaaagccggg aagtggaggt caccggcgaa
     9061 aaagacgtcg tcgttaccgt cgccctggac aagtctcgtg ccattggagg tcctgccgta
     9121 gctccacctc gtgtcaccaa agaagatccc aaaggacaca agcccccgga gacggctcca
     9181 ccccctactt catcccctcc gccaccaaag tcaacgaaaa cacaagaacc gaatgcgcgt
     9241 gatcgcattc gcaatctcga caaaagtaac ccatgggagc aatgagaggc atgcactata
     9301 gaaaaatcgt tgcgatgggc atcgtcctcg gcgccatggc gtcaccgggt ttcgtaagtg
     9361 cacaatccgt gggaacgcca gccgcacagc aggaagccat gcagcgcttc cagcgcgccg
     9421 tggacctata cgaagagcaa gactacgccg ccgcactcac ggaattcagg aaagcttacg
     9481 aattggcgcc ttcctacaaa ctgctctacc agattggaca ggtatcctac aaactgaagg
     9541 attatgcggg cgctcttcag gcttttgaaa aatatttatc tgaaggaaaa gatgaaatta
     9601 cgccggagcg gcgagctgag gtgcaagagg aaatcaactt gctcaaaaac caaaccgggc
     9661 atctcgaaat caactccaac gtatttgggg cggcggttac cattgatgac aatcccgtgg
     9721 gcaccatccc tctcgacggg cccatccttg tcagcgtggg aagtcgaaaa attaccgtga
     9781 gcgcggaagg tcgacagtcc gtgacccaag cgattacggt ggctgggcag gagcttcgga
     9841 aactcaattt cgatttgcct gaactcgtcg gtgagacccg gacggtctat cagaaagaaa
     9901 gtccttcaaa aatgaccact tggtcttggg ttggcgtcgg agtggcgggc gcgctgggca
     9961 ttggcgccac catcaccggg atcatggcca tgaacgcgga cgaggatttg tcgaacatga
    10021 cctttgtggg gggaacgcca gattcgaaaa tcaaagatca gcaaagcaaa gtcgataatc
    10081 ttgcgctaac gacggatatt ctcgcggggg cggctatcgt tacccttggc accacgttgg
    10141 tttggacttt cgcccggccc gatgcgccac ctgaattcga tagcgagcca cctcccgcgg
    10201 aagcaaaacg tcctttcatc caacccatga tcggcctcgg ttcgatcgga gtcacaggag
    10261 agttttgaaa tgcgtcgtac gaacttgctg cgcaccctcg ctacgacctt tctcagcgtc
    10321 ggcttcgccg ttgccgcagc ttcgagctgc tcgctgatca ccagcagcga tattgcccct
    10381 ggcggtatcg gacagccttg caaagacgat tccgattgtc agggcgcgag ttgtgacaac
    10441 gggctttgca cggcggcttg cagtaccacc gcccaatgcc cggaaccatc ccagtgcttg
    10501 aacaatacat gccagctccc cttgcgtgtc gcgggcattt ggatcggctt ggccactgcc
    10561 gcggaaggat ggaccctcac ccatcaggaa ggtatcgaat tcgccaaaga aaaactggga
    10621 tatatcgatt acgtctatga ggaaggtgtc cttggggagc cggccgaaaa ggccatcgat
    10681 aagtatgtcg ctgatggcta tcaggtcatc atcgccaact cctgggatca ccttgccacc
    10741 gttcggaaaa aggctgccca atatcccaat accacctttt tacattgttc aggaattcca
    10801 gtcgagccta atgtctccag ctttttcgca cacatggaac acgcctggta cgtcgctggc
    10861 cgcatcgccg cgcgaaaaag caaaacaggc caccttggct tcattggttc tttcgtcacc
    10921 gccgaagtcg tccgccacct caatgccttt gccctcggcg caaaaaaaga aaacccgaat
    10981 gccaaagtcg aggttcgctg gctcggattc tggtacgacg tcgacttcga caaagccctg
    11041 tgggaataca cgcccaaaca catgggcgac aaagccgtca agaaaaacat gaccggtgag
    11101 gaatatctca ccgccaaatt gatcgactcc ggcgccgatg tgattaccca taatctggat
    11161 aatcagcttc cttctagata tgtgggaatt catacaacgg ccaaaacaat gaaaaatcca
    11221 aaaaatcccg atcaggattg ggtggtctgg accatcgcca acgacaacat ccacggctgg
    11281 cgagaccaga gcgggcagcc cttcgccaac gccattggtg ccacctactg gaattgggga
    11341 tacccctata tcgagatatt cgaggacatc caccggggcc ggctcacctc caaggcctat
    11401 atgtacccac taacgaccga tacggaaacc tcggtcg
//
LOCUS       DAOSOR010000126        11421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5660_length_11421_cov_4.607426, whole genome shotgun sequence.
ACCESSION   DAOSOR010000126 DAOSOR010000000
VERSION     DAOSOR010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11421
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5660_length_11421_cov_4.607426"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="PK710_08980"
     CDS             <1..874
                     /locus_tag="PK710_08980"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOT09891.1"
                     /translation="FANTAFPPPNFDPPHERSAREGDGRWVRMGDERKNESAAKEPAV
                     LYRTTVHPHPVSKFISVTIAAMDLKHTSLHLVAGTDDPGADKAPRTRGLVPLEHQSDL
                     VLVHNGGFKPQHGNWGMMAEGEWVVPTRDEGCTVLITNDDRVVMGTWSSLSAHAPQAR
                     SIRQTPPCLVEANAIHPKLLAGDARAWGGTAKDIKTRRRTAIGIDASGRVLLFGMGEE
                     ADPKLLGEAMKVAGAQHAAQLDINYNWTRFLLFGTPDGVLQVTSTLIPKMVHRKRGYV
                     ALSEDRDFFYLKRR"
     gene            complement(890..4195)
                     /gene="ygfK"
                     /locus_tag="PK710_08985"
     CDS             complement(890..4195)
                     /gene="ygfK"
                     /locus_tag="PK710_08985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HOT09892.1"
                     /translation="MEKKFEPAPIDQLTRWIFGGLKSDEIMGMPKSSVRVPQPRMTMT
                     CSDQRLGAPLGVAAGPHTQLAQNIVASWLFGARFIELKTVQILDEIKVARPCIDSADV
                     TYNCEWSQELTLEQSFQEYLGAWVLIHALAHELGHPHDMFFNMSVGYDLDGIRSEKVQ
                     RFLRRMRDASEDIKTVVDAVATVVPSVRDIPIDPHLSNLVTLSTMHGCPPAEIERIAR
                     FMLEDLGLDTWVKLNPTLLGPDLLRGILNERMGFDIVVEDSAFDHDPKFDDAMQMVRN
                     LSAAAQKCGRRFGIKLSNTLEVSNRRPVFPSSEKSMYMSGRALHPLTLTLAHRVTEAL
                     DGKVPISFCGGADAFHFPSLVADGLGPVTTCTDLLKPGGYARLAQYLDSLAEAMDAVS
                     ASSIDEFVLARAGLHGADPSAAARENLARHAEQVLGDKSMRRRIKPLETKGSRPLGEF
                     DCIAAPCQEACPTHQNIPDYMRLVALKRPSEAMDVILHTNAMPAVTGRVCDHPCIERC
                     VRNHYEAPLAIRAIKRHAVEQGNPNSQEEARTKRGIRVAVVGAGPAGLSAAYYLARAG
                     CSVTVFESKPEAGGMASAVIPSYRLPDDPIALDLDRIRQAGVWIQFGTSVGRDVTLHD
                     LRKQGFEHLVLAAGAQKGRTLGIVGEEANGVFDALEFLEAVRHGKAPAIGPHVVIIGG
                     GNSAMDAARTAVRLVGLSGRVTVLYRRTIEQMPADPQEIQAALDEGVSLSVLRAPVKV
                     VQRNGCVTGLICTEMKLGEADRSGRPRPIPVEGSEALLTCDTILVAISQEPILNFLSD
                     VQPKMWKDGTLIVDAETGETSLENIFAGGDLVRGPSTVIQAIADGRRIAHTIAQRENL
                     VWDEPPIAPDRLSQKDLLERRSTRVSAIAEPELAIEQRNGFKEVMTGLPVSQAILEAD
                     RCLACDTMCSLCVTVCPNRANQMYVIEPLHAELPSLCVDGGELIVGGTQAFHVTQPYQ
                     IINIADFCNECGNCTTFCPTSGDPFRDKPRVHLRDDGFDNAPYDAYRIRPSTNGVEVL
                     AKLDGSQHRLQIEGDQLRYRGQGVQVLFDSQLGMKEAEALEPLEEGTEIDLSIAARMM
                     LLARALNVIPIGG"
     gene            complement(4237..6078)
                     /locus_tag="PK710_08990"
     CDS             complement(4237..6078)
                     /locus_tag="PK710_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09893.1"
                     /translation="MIPHTASALEAGDVGGHPLTIDVTETAVVNYNVDNRNQTGLDDD
                     WGTWMNRFNVQAQWHHFVFGVRMDNSMFFATPDPNDLAEEDVAAWSPSPGNPDAPDLY
                     ERTLVYGRELSNRYRNVVYPSKLVLSYITPEVEATVGDVYAQYGRGLVLSVRKIDELA
                     VDTTIRGGKISYRVPMPKGTKLRISGVGGYLNPLRVDEASGRVLEAPSHWYFAGMPTP
                     RQTAYIADPQPTFVPDRLVGASVEGGPTQATVGFHAVALDRPDRCSSAADSKDCTPYG
                     NQHARGADQIRNASVSLTVPDIFEHGSVYVEAALQQQRNKNFRAPSGGFGRTTADDMD
                     GHAVYASASANGGPFALTLEGKHYRKFFPLEANIDADGAVNEFALIQYSAPPTTLPVY
                     VDTEGNFFNTCVTGGRGRLDARVTDDLLVYGWLGRYATWGETGTAACEIRDDNRNDVW
                     DSAVGLEATLENKKSHIFAWIGARGDQLAEPDPVSEPTSVYYREGYLRYDLLKQLSRV
                     LYLQVQGVARRRHNVAETPIPWWEGETYTALQWNPHLSVAFGYEYTSQEGVPDSYYNG
                     SVKWRVDSDKSLRVFVGQQRGALRCISGVCRQFAPFEGAKAEAIVRF"
     gene            complement(6399..6818)
                     /locus_tag="PK710_08995"
     CDS             complement(6399..6818)
                     /locus_tag="PK710_08995"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HOT09894.1"
                     /translation="MQDLGEAITRDLDDCALEALIEVVYLAAFADGTFSDAEREHFTT
                     SFLRLTDGRSEPPRPEIIVDSLRKKLETEGRQACLSSIRERLPTPNLRWLAVMLAADM
                     TASDGVIHPSEREILLALAQILDVDIEEATKLVDGFE"
     gene            complement(6875..8167)
                     /locus_tag="PK710_09000"
     CDS             complement(6875..8167)
                     /locus_tag="PK710_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOT09895.1"
                     /translation="MRAAIFPYVLLSSLVGCSSLLDFDAVSFESSDGGGQSESAAGGT
                     GGGNDSGASGGAPTGGAGGTGGGEETGGVGGTGGELVDASIESGTGGVAGASASGGAA
                     GATGGSGGNSDPCATITCGVDEHCEPATLSCVCNPGFVSTGGGCEPSLPGDPAQHTEQ
                     EVCDQWNQGHSLTDPSPWSAGPSQCDPGTLTRAGIDDTLVRINLFRWLVGLGPVSDST
                     TLNEMDMWCAVVAAWNPPGGGNNPHSPDPSAKCYTQQGASGAGQSNIAWGSGHPAQAI
                     DQFIQDNGNFTTFGHRRWIFNPPLGPVGIGYYGGGGPYGNAQCLAVFGSSGSGPSPDW
                     IAFPPPGFVPTAIATWVWTFHHKSGVSSAEMSVVRQSDAESLAMEKMPLTGGYGSYPT
                     VAFRPSGWQLAAGETYIVTVDGVGGGSITYEMKPVTCN"
     gene            complement(8167..9126)
                     /locus_tag="PK710_09005"
     CDS             complement(8167..9126)
                     /locus_tag="PK710_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09896.1"
                     /translation="MRSRRLMWLFLGFVAILGLASPAWAQASWKDKAEAQKLVNEGLK
                     HDKAEAWQEAREAFEAAIALNDTPKARYYLAKALVNLRLLIEAREHAQAAADNPRAGW
                     WDRKNSRELLKKIEDSIAHITLRVPPDFQGKVYLGDVPLSSSDFGSRLEVNPGTIRIL
                     AESAGFFPFEKNMNLRDGADETITVELEPEPVDTVTQKEEEATLSTEGSSQKTWGYVS
                     LAVGGVGLVAGTAFGLMAKNTRNDLEAACIDDVCSENQRDAYDRGKMQAHISTAGFIV
                     GGVGLGLGAVLLLTGPKEAEKEAETARWQPYVGPTGAGIVGTF"
     gene            9252..10841
                     /locus_tag="PK710_09010"
     CDS             9252..10841
                     /locus_tag="PK710_09010"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOT09897.1"
                     /translation="MHPILFRIPVPNWNVPGVGPLHETFTQGNIPIYSYGVMLGLSLV
                     VGWYLTLWLAEKDGLPKETMANCYVVTALAAIVGSRVLYVVTNLEEFNGLGDFFAFRR
                     GGLVAYGGFLGGYLGSWGYLKLKKIPLMPWADIAVPSLASGLAITRIGCFLFGCDFGK
                     PLGDGAPEFLKKIGTFPHWAPGMVDRGDGAPAWIQHVNSGLIDHEATHSLPVHPTQLY
                     ESLVGLALLGLLMVQRKHQRFRGQVFMLFAFAYGFARFLLEIVRDDAERGLYGPELGA
                     HILIPGSLALFAVGYSIGFANGIPNVTMRRLTQALSFVPALVAFLMMRPASFMSASIV
                     QLSTSQWVGVLTGVAVSVAYMIFWKAAEAHPAEAMMINLKAFHAAEAAKANESNAEEP
                     ERDEDEDGEETEKPRTKGKKKGKKSSKEPIDATFDDEPEASSEKNKSVDTKTEDEPEA
                     PRKKGQVVDATFDDEPEASSEKDKSVDTKTEDEPEAPRKKGQVVDATFDDEPEASSEK
                     DKAVDAKTEDEPEAPKSDDDE"
     gene            complement(11127..>11421)
                     /locus_tag="PK710_09015"
     CDS             complement(11127..>11421)
                     /locus_tag="PK710_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09898.1"
                     /translation="AGISDCKTPGDAGCDGSDPHEGIDCCDASDPNCSCGAGTDADTD
                     ADADADADADTDADADADTDADADADSDVTEDGDADPSTDSHHDDDAVDATQS"
BASE COUNT         2406 a         3260 c         3277 g         2478 t
ORIGIN      
        1 ttttgcgaat acggcgtttc cgcctccgaa tttcgaccct ccccacgagc gttcggcccg
       61 cgaaggcgat gggcgttggg tgcgaatggg tgatgagcga aaaaacgaat ccgcagcgaa
      121 agaacctgct gtgctctacc ggacgaccgt gcatccccat cccgtgagta agttcatcag
      181 tgtcaccatc gctgcgatgg acttgaagca tacgtctttg catctggtgg caggaacgga
      241 cgatcccggt gcggacaagg ctcctcgcac tcgcggtttg gttcccttgg aacaccaatc
      301 tgatctggtg ctcgttcaca acggtggatt caaaccgcag catggaaact gggggatgat
      361 ggcagaaggc gagtgggtgg tgccaacccg cgatgagggc tgcaccgtct tgatcacgaa
      421 cgacgatcgg gttgtgatgg gaacttggtc gtcactatcc gctcatgctc cacaagccag
      481 gtcgattcgt caaactccgc cgtgcttggt agaagccaac gccattcacc cgaaattgtt
      541 ggctggtgat gcgcgtgcct ggggaggtac ggccaaagac atcaaaacgc gtcgccgcac
      601 ggccatcggg atcgatgctt caggacgtgt gttgttgttt gggatgggtg aggaagcaga
      661 tccgaagttg cttggcgagg cgatgaaggt tgcgggtgcc caacacgccg cacaactgga
      721 tatcaactat aattggacgc ggtttttgtt gtttggaact cccgatggcg tgcttcaggt
      781 aacctcgacg ctcattccga agatggtgca tcggaagcgt gggtatgtcg cgttgagtga
      841 agatcgggat tttttctacc tcaagcgacg atgaaagggg ccatgggcgc tatcccccga
      901 tgggaatgac gttcaaggcg cgagcgagca gcatcatcct tgccgcgatg gataggtcga
      961 tttccgttcc ttcttcgagc ggttcgaggg cttcggcttc tttcattcca agctgagagt
     1021 cgaaaagcac ctgcacccct tggccgcgat atcggagttg atccccttcg atctgcaatc
     1081 gatgttggga accatcgagt tttgccagca cttcaacgcc gttcgtgctg gggcgaatgc
     1141 ggtacgcatc gtaaggagcg ttgtcgaatc catcatcgcg aaggtgaact cgcggtttat
     1201 ctcggaaagg atcaccggat gtggggcaga aggtggtgca gttaccgcac tcattgcaaa
     1261 agtcggcaat attgatgatt tgatagggtt gggtgacgtg gaacgcttgt gtgcctccta
     1321 cgatgagttc tcccccatcg acacacaagc tgggcaattc ggcgtgtagc ggttcgatca
     1381 cgtacatttg gttggcgcga ttggggcaaa ccgtgacgca caagctgcac atggtgtcgc
     1441 aagcgaggca gcgatccgct tcgaggatgg cttggctcac gggcaagccc gtcatgactt
     1501 ccttgaatcc attgcgttgt tcgatggcga gttccggttc ggcgatcgcg gaaacgcgcg
     1561 tggaacgtcg ttcgagcagg tctttttgac tgagccgatc aggtgcgatc ggtggctcat
     1621 cccaaacgag attttctcgt tgagcaatcg tatgcgcgat gcgacgacca tcggcgatgg
     1681 cctgaatgac ggtggatggt ccgcgcacga gatcgccgcc ggcaaagata ttttcaaggc
     1741 tcgtttcgcc ggtttcggcg tcgacgatca gggttccatc cttccacatt ttcggttgta
     1801 cgtcggaaag gaagttgagg atgggttctt ggctaatggc caccaagatg gtgtcgcagg
     1861 tcaacaaagc ttccgagcct tcgacgggaa tgggtcttgg ccgtccggag cggtcggctt
     1921 ctccaagctt catttcggtg catatgagcc cagtgacgca gccattgcgc tgcacgactt
     1981 tgacgggagc gcgaagtacc gaaaggctca ctccttcgtc gagagcagct tgaatttctt
     2041 gtggatcagc aggcatctgt tcgatcgtcc gacgatagag cacggtgact cgaccggaaa
     2101 ggcccaccaa acggaccgcc gttcgtgcgg cgtccatggc ggaattgcca ccgccaatga
     2161 tgacgacatg cgggccgatg gcgggtgctt tcccatgacg tacggcctcg agaaattcga
     2221 gtgcatcgaa tacgccgttc gcttcttcgc ccacaatgcc gagggtacgc cctttttggg
     2281 ctcccgccgc aagcacgaga tgttcgaatc cttgcttgcg aaggtcgtgc agcgtgacgt
     2341 ctcgcccaac actcgtgcca aattggatcc atacccctgc ttgccgaatg cgatcgagat
     2401 cgagagcgat ggggtcgtcg ggcaagcggt agcttgggat gactgcggat gccattcccc
     2461 cggcttcggg tttgctttcg aagacggtga ccgagcagcc tgcgcgagcg agatagtagg
     2521 cggcggatag gccggcgggg ccggcaccca ccacggccac gcgtattcct cgcttggttc
     2581 tcgcctcttc ttgtgaattc ggattgccct gttcgactgc atgacgcttg atggcgcgaa
     2641 tggcaagggg cgcctcgtaa tggttgcgta cgcatcgctc gatgcaagga tgatcgcaga
     2701 cacgtccggt gacggccggc atggcgttgg tgtgtaaaat gacatccatg gcttcggagg
     2761 ggcgctttag ggccaccaac cgcatgtagt cgggaatatt ttggtgggta gggcatgctt
     2821 cttggcaagg cgcggcaatg cagtcgaact cgcccaaagg cctcgaacct ttggtttcga
     2881 gcggcttgat ccggcgacgc atactcttgt ccccaagcac ctgttcggcg tgtcgcgcca
     2941 ggttttctct ggccgcggca gagggatcgg caccatgcaa ccccgcgcgg gccaatacga
     3001 attcatcgat actcgatgcc gataccgcat ccatggcttc cgccagcgag tccaggtact
     3061 gcgccaaacg cgcgtatcca cccggtttca aaagatcggt acacgtggtc acggggccga
     3121 gaccatcggc caccaacgat ggaaaatgga acgcatcggc acccccacag aacgagatcg
     3181 gaaccttgcc atcgagcgct tcggtaacgc ggtgggcgag tgtcagcgtc agcggatgca
     3241 acgcccgtcc cgacatatac atgctctttt cactagaggg aaagacgggt cggcgattgg
     3301 atacctccag ggtattggac aatttgatgc cgaaacggcg tccgcatttt tgggctgccg
     3361 cggaaaggtt gcgaaccatt tgcatcgcat cgtcgaactt cggatcgtga tcgaaagccg
     3421 aatcctccac gacgatgtcg aaccccattc gttcgttgag gattccccga agcaggtccg
     3481 ggcccaacaa ggtgggattg agtttcaccc acgtatccaa cccgagatcc tcgagcatga
     3541 aacgcgcgat tctttcgatt tccgcgggag gacaaccgtg catggtcgat agcgtgacga
     3601 gattcgacag atgtggatcg atgggaatgt cccgcaccga tggcaccacc gtcgccaccg
     3661 catcgaccac ggttttgatg tcctcgctcg catcgcgcat acgacgtaaa aaacgctgca
     3721 ctttttcaga gcgtatgccg tcgagatcgt agccaacgct catgttgaag aacatgtcgt
     3781 gggggtggcc gagttcgtgg gcaagggcgt gaatcaacac ccaagcgcca agatactctt
     3841 ggaaggattg ctcgagggtc agctcctgag accactcaca gttgtaggtc acgtcggcgg
     3901 aatcgatgca ggggcgcgcc actttgattt catcgagaat ttgtaccgtt ttcagctcga
     3961 tgaatcgtgc tccgaagagc caggaagcga cgatattttg tgcgagttgg gtatgaggtc
     4021 ccgcagcgac gccaagagga gcgccgagcc gctggtctga gcaagtcatc gtcatccgag
     4081 gttggggaac tcggaccgaa gacttcggca tccccatgat ttcatcgctt ttcaagcctc
     4141 cgaaaatcca gcgggttagc tgatcgatcg gtgcgggttc gaacttcttt tccattcact
     4201 ctctccaacg ctgtggctcc cagggagcat aagcgatcaa aatcgaacga tagcctcggc
     4261 tttggcaccc tcgaaaggcg caaattgccg acatactccg gagatacaac gcaaagcgcc
     4321 acgctgttgt cccacgaata cacgcaagct cttgtccgag tcgacacgcc acttgaccga
     4381 accgttgtag taagaatccg gtaccccttc ttgcgaggta tattcatacc cgaaagccac
     4441 ggataaatgt gggttccatt gcaaggccgt atacgtttct ccttcccacc acggaatcgg
     4501 tgtttccgcc acgttgtgac ggcgacgcgc cactccttgc acttgtagat aaagcacgcg
     4561 tgacaattgc ttgagcaagt cgtagcgaag ataaccttcg cgataataca ccgaggtggg
     4621 ttccgagacc ggatcgggct cggcaagctg gtcccctcta gcgcctatcc atgcgaaaat
     4681 atgcgacttt ttgttttcga gggtggcttc tagccccacg gcggaatccc atacgtcgtt
     4741 gcggttgtca tcgcggattt cacaggccgc ggtgcccgtt tctccccacg ttgcatatcg
     4801 acccaaccaa ccatacacaa gcaaatcatc ggtgacacgc gcatcgagac gtccacggcc
     4861 tcccgtcaca cacgtgttga aaaagttgcc ttccgtgtcc acgtataccg gcaaggtggt
     4921 tggtggcgcg ctgtattgaa tgagcgcaaa ctcattgacg gcaccatcgg cgtcgatgtt
     4981 ggcttcgagg gggaaaaatt ttcgatagtg tttgccctcg agcgtcagcg caaatggacc
     5041 accgttggcg gaagccgaag cgtataccgc gtgaccgtcc atatcgtctg cggttgtgcg
     5101 accgaatcca ccggaaggag cgcgaaaatt tttgtttcgt tgctgctgga gggccgcttc
     5161 gacatatacc gaaccgtgct cgaaaatgtc tggcaccgtg agagatacgc ttgcgttgcg
     5221 aatttgatcc gcgccacgcg cgtgctggtt gccgtacggt gtgcagtctt tcgagtccgc
     5281 ggcgctggag cagcggtcgg gacgatcgag cgcgacagca tgaaaaccca ccgtagcttg
     5341 cgtaggtcca ccttccacgc ttgcaccaac gagtcgatcc ggcacgaagg tgggctgtgg
     5401 atcggcaata taagccgttt gccgcggtgt gggcattccc gcaaaatacc aatggctcgg
     5461 ggcctccagc acgcgcccgc tcgcctcgtc gacgcgcaaa ggattgagat atcctcccac
     5521 cccggagatg cgaagcttgg ttcctttggg catgggaacg cgataagaaa tttttccgcc
     5581 acgaatcgtc gtatcgactg ccagttcgtc gattttgcgc acgctcaaca ccagaccgcg
     5641 cccatattgc gcatacacgt cccctacggt cgcttctact tcaggcgtga tatacgaaag
     5701 gacaagcttg gaggggtaca ccacgttgcg ataacgattc gacaactcac ggccatacac
     5761 gagcgtgcgc tcatacaagt ccggagcatc ggggttgcct ggagaaggtg accacgccgc
     5821 aacgtcttct tccgccaaat cattgggatc tggcgtggcg aaaaacatgg agttgtccat
     5881 gcggacacca aacacgaagt gatgccattg cgcctgcacg ttgaatcgat tcatccacgt
     5941 gccccaatcg tcatccagcc cggtttggtt gcggttgtcg acgttgtagt tgacgacagc
     6001 ggtttcggtg acgtcgatgg tcagggggtg tcctcccacg tctccagctt cgagcgcaga
     6061 agcggtgtga ggaatcatgg tcgcgaccaa tgccatccag cacatccccc cccacactct
     6121 cggggctcgt ggcgaacggt acacccttgc acgcaccccg tcgcggttcg aggcagaagc
     6181 agcacgccat cgcatggcgc tcgttgtagt acagggctcg gcaagttggt acaggggcgt
     6241 atacgatcgt tggcaaagcg ttcacgcttc cgttcaacgt agcgcgatga ttcggatctc
     6301 cgcggaagat cctcggtgtc tcacggcgcg atcgctcgac attggctccg atgaggacaa
     6361 aaaatcgcgt ttcggtgaga agaacctttc cttcgacctc actcgaatcc atcgaccaat
     6421 ttggtggctt cttcaatatc cacgtcgaga atttgggcca acgccaacaa tatttcccgc
     6481 tccgaaggat gaatgacgcc atcactagcg gtcatgtctg ccgcgagcat gacggcaagc
     6541 cagcgtaaat tgggtgtcgg cagtcgctcg cgaatggagg ataaacacgc ctgcctacct
     6601 tcggtttcta gcttttttcg tagactatct acgatgattt ccgggcgtgg aggctcggat
     6661 cggccatcgg tcaagcgcaa gaaactggtg gtgaagtgct cacgctccgc gtcgctgaac
     6721 gtgccatcgg cgaacgctgc caagtacacg acttcgatga gcgcctcgag agcgcaatcg
     6781 tcaaggtcac gggtaatcgc ttcgcccagg tcctgcatac aaaaggattg tattggtgat
     6841 tacccacacg agcaaatgag tcgtgcatga gctttcagtt gcatgtcacg ggtttcatct
     6901 cgtacgtgat cgagccacca cccacgccat cgacggtcac gatgtacgtt tctcccgcgg
     6961 cgagttgcca gccggaagga cgaaaagcca cggttggata agagccgtaa cccccagtca
     7021 aaggcatctt ttccatggcg agagactcgg cgtcggattg tcgaacgacc gacatttcag
     7081 cggaagacac gcccgacttg tgatggaacg tccaaaccca ggtggcaatc gcggtcggaa
     7141 cgaatccggg tggcggaaac gctatccaat cgggggaagg accggacccg ctcgaaccga
     7201 agacagctag gcattgcgca ttgccatagg ggccgccgcc accgtaataa ccgatgccaa
     7261 caggaccgag gggtggattg aaaatccatc gacgatgccc aaacgtggtg aagtttccgt
     7321 tgtcttgaat gaattgatcg atggcctggg ccggatgacc cgagccccag gctatattcg
     7381 attgtcccgc cccgctggct ccctgctgcg tatagcactt ggcactcgga tcgggcgaat
     7441 ggggattgtt tccgcctccc ggaggattcc acgctgccac caccgcacac cacatatcca
     7501 tctcgttgag tgtcgtgctg tccgagacag gaccgagccc caccaaccag cgaaaaagat
     7561 tgatacgaac gagagtgtca tcaatgcctg cacgcgtcag tgttcccgga tcacattgcg
     7621 aaggacccgc gctccaggga ctcggatcgg tcagggaatg cccttgattc cattggtcgc
     7681 atacctcttg ctcggtgtgc tgcgcaggat cacccggcaa agaaggctca caccccccgc
     7741 ccgtggaaac gaatcccgga ttgcatacac acgacagggt cgcgggctca caatgctcgt
     7801 cgacgccgca ggtaatcgtg gcgcaagggt ccgaatttcc accacttcct cccgtggcac
     7861 ccgcagcccc gcccgacgcg ctagcgcctg caacccctcc cgttccgctt tcgatactcg
     7921 cgtcaacaag ctcccccccc gttccaccca ctccccccgt ttcttctcct ccgcctgttc
     7981 ccccggcacc gccagtcgga gccccgccag atgccccaga gtcattgcca cctcccgttc
     8041 cgcccgcggc actttcggat tgcccgcccc catcgctcga ctcgaaagaa acagcatcaa
     8101 agtcgagcaa cgacgaacac cccaccaacg atgacaataa tacgtagggg aatatggccg
     8161 cacgcatcag aaggtcccaa cgattcccgc cccagtcggc cccacatagg gttgccatcg
     8221 agcggtttcc gcttcttttt cagcttcttt tgggcccgtg agcaacaaca cggctccaag
     8281 gccaagaccc actcccccga cgatgaatcc ggctgtcgag atgtgtgcct gcatttttcc
     8341 ccggtcatag gcatcccgtt ggttttcgct acagacatca tcgatgcatg ccgcttctag
     8401 gtcattgcgc gtgttttttg ccatcaaccc aaaagcggtt cctgccacga gtcccacgcc
     8461 gcctaccgcg agactgacgt aaccccaggt tttttgtgaa gacccctcgg tcgatagcgt
     8521 cgcctcctct tccttttggg taacagtgtc gacaggttcc ggctcgagtt cgacggtgat
     8581 ggtttcatcg gccccatcgc gcaggttcat gttcttttcg aaaggaaaaa aacctgcgga
     8641 ttccgccagg attcgaatgg tgcccggatt gacctcgaga cgagaaccaa aatccgaaga
     8701 cgaaagcggc acatcgccta gatagacttt tccttggaaa tcagggggta ctcgcagtgt
     8761 gatatgcgcg atgctgtcct cgattttttt gaggagttca cgagaattct ttcggtccca
     8821 ccaaccggct cgcgggttgt ccgcggcggc ttgtgcgtgt tctcgcgctt cgatgagcaa
     8881 acgcaagttc accaacgcct tcgccagata gtatcgagcc ttgggagtgt cgttcaaggc
     8941 tatcgccgct tcgaacgcct ccctcgcctc ctgccaggcc tccgccttgt catgtttcaa
     9001 cccttcgttg acgagcttct gcgcctccgc cttatccttc caactcgctt gtgcccacgc
     9061 cggtgacgcc aagccgagaa ttgccacgaa cccaaggaac aaccacatca aacgtcgact
     9121 gcgcatccaa taagaattcc agatgccccc acggtccgtc aacccgagcg tgcaattcta
     9181 cccccgcccc gtgttcatca aattcgtcgc aacactggca tgcgagtcgt ttcgattgta
     9241 agaacggccc catgcatccc atcctgtttc gcattccggt gccgaactgg aacgtgccag
     9301 gggtcggacc cctgcacgaa acgtttacgc aaggaaatat ccctatctat tcctacggcg
     9361 tcatgcttgg cctgtccctg gtggtggggt ggtacctcac cttgtggctg gccgagaaag
     9421 acggacttcc caaagagacc atggcgaact gctatgtcgt caccgcgttg gccgccatcg
     9481 tcggttcccg tgtgctgtac gtcgtgacga atctcgagga attcaacggc cttggggatt
     9541 ttttcgcgtt tcgtcggggg ggattggtcg cttatggcgg gtttctcggc ggttatctcg
     9601 gctcctgggg ctacttgaaa ctcaagaaaa ttcctctcat gccgtgggcg gatatcgcgg
     9661 tcccttcctt ggcatccggg ttggccatta cgcgaatcgg ttgttttttg tttgggtgcg
     9721 attttggaaa gcccttgggt gatggtgctc ccgaattctt gaagaaaatc ggtacgttcc
     9781 ctcattgggc tcccggtatg gtcgatcggg gagatggcgc tcccgcctgg atccaacatg
     9841 tcaacagcgg gttgatcgac catgaagcca cgcattcgtt gcccgtgcat cccacgcaat
     9901 tatacgaatc gttggtgggg ctcgctcttc tgggcttgtt gatggtgcaa cgaaaacatc
     9961 aacgctttcg cgggcaagta ttcatgctgt tcgcgttcgc gtacggtttc gcccggttcc
    10021 tgttggagat cgtgcgtgat gatgcggaac gcggcctgta cggaccggaa ctcggcgcgc
    10081 atatcctgat tcccggctcg ttggctctgt tcgcggtcgg ctactccatc ggtttcgcca
    10141 acggaattcc gaacgtaacc atgcgacgat tgacccaagc gctttcgttc gtccctgccc
    10201 tggtcgcttt tctcatgatg cgtccggctt ccttcatgtc cgcgagcatc gttcaattat
    10261 cgacatcgca gtgggtcggt gtgttgacag gcgtggccgt ttcggtggcc tatatgattt
    10321 tctggaaggc tgcggaggcc cacccggctg aagccatgat gatcaacctc aaagctttcc
    10381 acgcggcgga agcggcgaag gccaatgaat ccaacgccga ggaaccggaa cgagacgaag
    10441 acgaagacgg agaagagacg gagaagccgc gcacgaaagg caaaaagaag ggcaagaaaa
    10501 gctcgaaaga gcccatcgac gcgacgttcg atgatgagcc ggaagcctcg agtgagaaaa
    10561 acaaatccgt cgacaccaag acggaggatg agccggaagc cccgaggaaa aaggggcaag
    10621 tcgtcgacgc gacgttcgat gatgagccgg aagcctcgag tgagaaagac aaatccgtcg
    10681 acaccaagac ggaggatgag ccggaagccc cgaggaaaaa ggggcaagtc gtcgacgcga
    10741 cgttcgatga tgagccggaa gcctcgagtg agaaagacaa agccgtcgac gccaagacgg
    10801 aggatgagcc ggaagccccg aagtccgacg acgacgaata gcgatctgcg cttgcggcgg
    10861 ttaggtccgc cgtcttgttt tcattaaaag aatcgatgag atagagccgt ggccgtgtag
    10921 gaacgatttc gtgttcccac gaattccgag gcgtcggtcc tgggcccgtc tcgatgggaa
    10981 gcggaatgga tcatgctaat cgtttacgta tagtcaattg gctgtatacg cgatcgactt
    11041 caaggatata tatggtaaga acagtaagaa ttgattgaag cggatgtggc ttcgctatcg
    11101 attcattgta tacgaatacg tatatgtcat gattgggtgg cgtcgaccgc atcgtcatcg
    11161 tggtgggaat cggtggacgg gtcggcatcg ccatcttcgg taacgtcgct gtccgcgtca
    11221 gcgtccgcgt cagtgtccgc gtcagcgtcc gcgtcagtgt ccgcgtcagc gtccgcgtca
    11281 gcgtccgcgt cagtgtccgc atcggtgccg gctccacacg agcagttggg gtctgaagca
    11341 tcgcaacagt cgattccctc gtggggatcc gagccgtcac aaccggcgtc tcctggggtt
    11401 ttgcagtcgc tgatgcccgc g
//
LOCUS       DAOSOR010000127        11327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5724_length_11327_cov_4.859741, whole genome shotgun sequence.
ACCESSION   DAOSOR010000127 DAOSOR010000000
VERSION     DAOSOR010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11327
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5724_length_11327_cov_4.859741"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PK710_09020"
     CDS             complement(<1..475)
                     /locus_tag="PK710_09020"
                     /inference="COORDINATES: protein motif:HMM:NF020904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HOT09899.1"
                     /translation="MRTTARSWRRALHRIVSLADKAGDPVDFYPPRWSEEEMTRHFAF
                     IEASRGFRLPPSYKEFLSEFDGCHQLFRGAALFSVAQLLDPTHANAADDAIEDLNTPI
                     PSFVTPVQPHWRRDGLICIGLDAAGDVVFVLDPASVRDDGEMDVVAWFSGLGVRLP"
     gene            complement(590..1663)
                     /locus_tag="PK710_09025"
     CDS             complement(590..1663)
                     /locus_tag="PK710_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOT09900.1"
                     /translation="MQTEKRIYIGLAVLLVIGGLIYWQSKKSKEEAAARSVSIASSAT
                     LPSIKVPEEGVEKITKLVVQDKDKPEVVLEKRGEQWWVVKPLEAIASQQNVKSTLDNL
                     KAIETTDAINDTPEAPAMYKDYDLEGDKAIHVTAFEGEKGAEKVFDTFFGKSGSRGQM
                     ARLADKPGIWVVKGFSSFQYTRQLKDWREKSILKFEDKNVVSVTIDNEEGSFSFSQNA
                     EQWSATRNKKAIDKFDAEKIKDMLRAFRALNAEDFADDKKDKPEETGLGEKPVAVVTI
                     VLKDDAGTLKLVFGNTSSGSSRYAKKDGDETIYILGSWASDWAVAKVDKFQKVEKKDD
                     GKEKDDNKPPVDMPPFEMPGMGD"
     gene            complement(1674..3536)
                     /locus_tag="PK710_09030"
     CDS             complement(1674..3536)
                     /locus_tag="PK710_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOT09901.1"
                     /translation="MAMERRAKAATETGIYLFVLLGIVVGINVLSHGCGAIRGVYGRF
                     DATRTERFTLSQGSGRLLGTLKQPMDVEVYVTRGLAKLDAFVFDLEDLLKEYERAGGG
                     KFRYKLIEPKTDEQREAAKEAGLREAAFGEGSETGEDQASIKQGYMGIVFKYGSEKDT
                     IPILSPERTDGLEFWITNKIRELRDKADNISHRIGVLSGKDEIKLTEANLIAGQGGRG
                     GPSMQSIIQQAFPFYKFEDVDLKGGDTEIDEGIVGLIITQPGKELTSKELRRIDQFVM
                     RGRSLLVVAGAANLKSSESTMKASLNLQGLDKLLSGYGLEVSKDVAMDWGRLMRLPMM
                     TQSGNVQWIFLPGIAQVQYLPGITAEQQMLDNSFAAFFRIDEIAFPYPSSVTLHADKQ
                     PDAKLSVVARTSPRTTVVSGESAELRPSADVRPQPPFEQRAIAAVVEGKLKRAMDPPA
                     ESDGIEVPVTSAADARVLVIASGQFLANPFARAGQGPDLGPQFAMMGNIGGDEMLQAI
                     SQPYAQKYLTTTILALKNTLDWMSGDSDLIAVSAKILGEPNLTYANIQPPNLTENASE
                     DEIKAEIERVKAARKKTQTSVQWTLTLLLPAMFGLLGIGLWHLREKKRSTLSLD"
     gene            complement(3540..4274)
                     /locus_tag="PK710_09035"
     CDS             complement(3540..4274)
                     /locus_tag="PK710_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT09902.1"
                     /translation="MKGTLAIAGREFRSYFYSPLAYVVICVGLFLLGIFFFLFRGGFW
                     QIDRASFDRMFQYGALGLSLFIIPVITMRLLAEEKRSGTLEMLITLPVKDTEVILGKY
                     LGALGLVLVLLLGTALYPIFMFGWPWNLGPLDPGPVLAGYLGLILFSAAAIAIGLMVS
                     AFTESQTVAFFITFVALLILHNLSTIGEFAPGAIGEFLRVLSFDTNLQKFARGLIDTR
                     NVIYFLSITGLCLVMAFRTLESRKWS"
     gene            complement(4271..5371)
                     /locus_tag="PK710_09040"
     CDS             complement(4271..5371)
                     /locus_tag="PK710_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT09903.1"
                     /translation="MSTDVMIHADNLTKMFGSLTALDRVSFDVRRGEVVGFLGPNGAG
                     KSTTMRILTCFISPTAGTAKIHGFDVFDQPLEVRKKLGYLPQRAPLYHDMTVWSYLEF
                     CAAVREIDGSEFKKRLKKSAEICGIAQVLGKDIRTLSHGYRQRVGLAQALIHDPPILI
                     LDEPTSDLDPNEKTEVINYIREIGRERTVLLSTHNLSEVESACARAVIVSKGRVVADG
                     TLDDIRAKSGRTRYVVSVHEKKAQGDQVAPTAQQVADALSSIVGVSTVRETPSDDTTH
                     TFTLQAAGDVDVRSDIFQLMVDKGWLLIELHRDAQSLGDVFRDLTKSESRMARGFRAE
                     PEEDEEDEEDEDEDEDQDEDEDDSDEEEDEKS"
     gene            5661..6032
                     /locus_tag="PK710_09045"
     CDS             5661..6032
                     /locus_tag="PK710_09045"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOT09904.1"
                     /translation="MGPLTTYLIETFVTLVAVLALAFLILMGAKRFGIGQPIGPITLV
                     GRLPLDTRRAVYLVRVANQVIILGSSEAGLCKLGEVQEQHIEDFKNPQTQPRFSDLIA
                     RFRQSSRRVSQGSTAPDNDHG"
     gene            6025..6870
                     /locus_tag="PK710_09050"
     CDS             6025..6870
                     /locus_tag="PK710_09050"
                     /inference="COORDINATES: protein motif:HMM:NF009438.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HOT09905.1"
                     /translation="MASPTFAELAPTSRPSPIDAFAAKTNHVAHASTRALAPLLTLLI
                     VLFSAGPAWGAPDGSPDPLLARPIALIIALALVSLAPFVFMTLTAFLKISTVLHIVRG
                     AIGAQNIPSNTVVMALAAALTLIAMAPVGTRIMQRAAPVLADSDSANTATLVRGLVDA
                     TKEPLRAFLRANASPTEHGRFYEVARAARPPQERDDVARDDFIVLIPAFMITELMEAF
                     ALGFVLYLPFLVIDLVIANVLLSLGMQMLNPTQVSLPFKLLLFVAIDGWGLLARALVT
                     GYAVG"
     gene            7019..7972
                     /locus_tag="PK710_09055"
     CDS             7019..7972
                     /locus_tag="PK710_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09906.1"
                     /translation="MKRYLGFLPVGAIAVAGLVGLSIQAGCASDEDPVNNAGTGGGGQ
                     TGGSGGGEQTGGSGGGESGSGGGSPTGGSGGGSSQCPVGQAATVADVADATTTGIQVD
                     IKGAIATTKKLVVYFNKKKGSCLWGVFVKDSAADRGLMVISYGDNAPADSSQDKCPTG
                     TDDIPDGIVPGDVLDIVGQTDAYAPSTCTGAAKQRQLKACSVTKTGSGNPPAPVVVTD
                     LNALHNGDAKYQGLLVKIENVTAENFDGGAVGPYGVIELEGTDLQINDKFYYSESGAP
                     QFDPSQQFKHIIGISHLDYCDWVLQPRDKCTDFDPASKDCI"
     gene            complement(7987..8517)
                     /locus_tag="PK710_09060"
     CDS             complement(7987..8517)
                     /locus_tag="PK710_09060"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOT09907.1"
                     /translation="MRRILLFTSWLVAVLLAIASRAEAFERQWHAGLDAGFAALSWND
                     ELRSGLGGGLHAAYGLTDSYNALLEVGVSSHSVVAGQPMLVASHAALGMAYTLDVVSW
                     VPYVGLLVGGYQLSGFELRQAEYRLGFQAALGLDFRPSRSWAVGAQLRYHTFAQEPLQ
                     AHYMTTFLRFEWLWGW"
     gene            8555..10099
                     /locus_tag="PK710_09065"
     CDS             8555..10099
                     /locus_tag="PK710_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09908.1"
                     /translation="MSKTNSLEVHSTLAETPARRYLLCMRAVVSKWLIGWMVACAGCS
                     SSEADSTNNYANSSDGSFVDSSSDSADASPAVDAAVDATGDTSVDTVPDTAQDHAASD
                     SPSEDSLLDTTVDAEQDTPPDAPPWIVGNQATCPELAPLRDTSTDASSILQMCIDRTP
                     DGMTLVLDPGRYSLAAQLHLDREIGLSTTGRIGTASCSTEPTHGCAELFALPAFDTRL
                     GMFLATGAVTIDHIVLHGNRQGRSGTHAHQMCSALTDNAYGYNATLQCSNCRMTHSVS
                     MYALCGTGLQVIAPAVNVTISHNTFAFNGLHTTKNMWSDGLTVHDSEKGVYEHNTFID
                     NTDIDLIFGGCKKCKIRYNQVHHTSDPAGGAFAAIMIQKWPTTSGCYEDVDISGNAVD
                     CGPLRACGSAFYIGSESWYPETPYGTLQNGTTSGLIKGNSVNRAMNAFYIAARGLAIY
                     GNDFLQAHGVPIPNSCHKNIVSTTPIVVSPTAASCHFNFENVDPGMSIHYSSASWAGC
                     VPNYPF"
     gene            complement(10350..>11327)
                     /locus_tag="PK710_09070"
     CDS             complement(10350..>11327)
                     /locus_tag="PK710_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOT09909.1"
                     /translation="RLRPLQDHANDPEFQKAFMAVKHANKIDLTEEIKASCGLVVDPN
                     AIFDVQVKRLHEYKRQHLNLLYILTLYRRLLHDPDLPINKRVFIFGAKAAPGYDLAKR
                     IIRAINAVAAKINDDPRIQDKIKIAFLPNYSVSLAEKIIPATDVSQQISTAGKEASGT
                     GNMKLALNGAVTIGTLDGANVEILEEVGSDNIFIFGNTVQQIDELRRSGYDARLIYQR
                     NPELKAVLDWLHSDVFAPAGELSPVVDSILVHGDPFFVAADYEAYCQTQDEVDKAYRD
                     KARWARMAITNTARVGKFSSDRTIREYARDIWNLEPVFPPEQPLVKAAQ"
BASE COUNT         2386 a         3334 c         3146 g         2461 t
ORIGIN      
        1 agggcaggcg tacccccaat ccggaaaacc aggccaccac gtccatctcg ccatcatcgc
       61 gaaccgacgc ggggtcgaga acgaatacga cgtcacccgc cgcatccaac ccgatacaaa
      121 tcaatccgtc tcgtcgccag tgcggttgca ccggcgtcac gaaactcggg atcggcgtgt
      181 tgaggtcttc gatcgcgtca tcggccgcat tggcatgggt ggggtccaac agttgagcga
      241 cagaaaacag ggctgcgcct cgaaagagtt ggtgacaacc atcgaattcg gaaaggaatt
      301 ctttgtaaga aggaggaagg cgaaaacctc gtgatgcttc gatgaaagca aagtgcctgg
      361 tcatttcttc ctcggaccac cggggtggat agaaatcgac agggtcacct gctttgtccg
      421 cgagcgaaac gatgcgatgc aatgctcttc gccagcttcg ggccgtcgta cgcatgcctc
      481 gaacctacgc aaaacggtat ggtggagcaa aaaagtgggg acggttgggg agagggagtg
      541 gtgggatgta ggaaggataa tccagcgagg tcagacgttg cgataaggct cagtcgccca
      601 tgccgggcat ctcgaacgga ggcatatcga cgggaggctt gttgtcatcc ttctccttcc
      661 catcgtcttt cttttcgact ttttggaact tgtcgacctt ggcgacagcc caatcagaag
      721 cccaggaacc gaggatgtag atggtttcgt ctccgtcttt tttcgcgtag cggctcgagc
      781 ccgaagaggt atttccgaac acgagtttca aggtcccggc atcgtctttg agcacgatgg
      841 tcacgacagc gacgggtttt tctccaaggc cggtttcctc gggtttgtct ttcttatcgt
      901 cggcgaagtc ttccgcgttc agagcccgga aggcgcgaag catatccttg attttttcgg
      961 catcgaactt gtcgatcgcc ttcttgttgc gcgtcgcgct ccattgctcc gcattttgcg
     1021 aaaacgaaaa gctaccctcc tcgttgtcga tggtcacgga taccacgttt ttgtcttcga
     1081 acttcaggat ggacttctca cgccaatcct tgagttgtcg cgtgtactgg aacgaagaaa
     1141 agcctttgac gacccagata ccgggtttgt ccgcaagccg ggccatttgt ccgcgcgaac
     1201 cgcttttgcc gaaaaaggta tcgaagacct tttccgcgcc tttttctccc tcgaacgccg
     1261 tgacgtgaat ggccttgtcc ccttcgaggt catagtcctt gtacatcgct ggcgcttccg
     1321 gcgtgtcatt gatggcgtcg gtggtctcga tggccttgag gttgtcgagc gtggacttga
     1381 cgttctgctg gcttgcgatg gcttccaggg gtttgacgac ccaccattgc tcgccacgct
     1441 tttccaacac cacctctggc ttgtccttgt cctgaacgac cagtttggtg attttctcaa
     1501 cgccttcttc gggtaccttg atggagggca aggtcgccga cgacgcgatg gaaaccgacc
     1561 gtgcggcggc ttcttccttg gatttcttgc tctgccagta aatcaaaccg ccgatcacga
     1621 gcaggaccgc aaggccgata tagatccgtt tttctgtctg catgggtcac acctcaatcc
     1681 agagacaggg tcgaacgctt cttctcacga agatgccaaa gaccaatgcc gagcaacccg
     1741 aacatggcgg gaagcagcag agtcaatgtc cactgcacgc tcgtttgcgt cttttttcgg
     1801 gcagctttga ccctttcgat ctcggctttg atttcgtctt ccgaagcgtt ttccgtcagg
     1861 ttaggcggct ggatattcgc gtacgtcagg ttcggctcac caagaatctt ggccgacacc
     1921 gcaatcagat cactgtcccc agacatccaa tcgagcgtat ttttcagcgc caaaatcgtg
     1981 gtcgtcagat acttctgcgc ataaggctga gagatcgctt gcagcatctc gtcgccgccg
     2041 atgttgccca tcatcgcgaa ttgaggcccg agatcggggc cttgccccgc ccgggcaaag
     2101 ggattggcca gaaattgccc ggaagcaatc accagcacac gggcatcggc cgcactcgtg
     2161 accggtacct cgattccatc ggactccgcg ggcggatcca tcgctcgctt gagcttgccc
     2221 tccaccaccg cggcaatggc tcgctgctcg aacggcggct gaggcctgac gtccgccgaa
     2281 ggacgaagct cggccgactc accgctcacg accgtcgttc gaggactggt ccgcgccacc
     2341 accgacaact tggcatccgg ttgtttgtcg gcatgcagag tcacactcga cggatacggg
     2401 aaggcgatct cgtcgatacg gaagaacgcc gcgaagctat tgtcgagcat ctgctgctcg
     2461 gccgtaatcc cgggcaaata ctgcacttgc gcgatgcccg gcagaaagat ccattggaca
     2521 tttcccgact gggtcatcat cggaagacgc atcaaacgcc cccaatccat cgccacgtcc
     2581 ttggaaacct ccaggccata tcccgatagc agcttgtcca atccctgaag gttcagggaa
     2641 gctttcatgg tggactcaga ggacttgagg ttcgccgcac ccgccacgac gagcaacgag
     2701 cgaccccgca tgacgaattg gtcgatgcga cgcaattcct tggaagtcag ttctttgccc
     2761 ggctgcgtga tgatgaggcc aacgatgccc tcatcgattt cggtgtctcc ccctttcagg
     2821 tcgacgtctt cgaacttgta gaaggggaag gcttgttgaa tgatggactg catggagggg
     2881 ccaccgcgac ctccctggcc tgcaatgaga ttggcttccg tgagcttgat ctcgtctttg
     2941 ccggacaaca cgccgatgcg gtggctgata ttgtccgcct tatcacgtaa ctcacggatt
     3001 ttattcgtga tccagaactc cagaccatcc gtgcgttccg gcgataagat cggaatggtg
     3061 tctttttcac tgccgtactt gaagacgatg cccatgtagc cttgcttgat ggaggcctgg
     3121 tcttcgccgg tttcgctgcc ctcgccgaag gcggcttcgc gcaggcccgc ttctttggcg
     3181 gcttcgcgct gctcatcggt tttgggttcg atgagcttgt agcggaactt gcctccaccc
     3241 gctcgctcgt actccttgag caagtcctcg aggtcgaaaa cgaaagcatc gagtttggca
     3301 agtcctctgg tgacgtaaac ctcgacgtcc atgggctgct taagagtgcc cagcaggcgt
     3361 ccactgcctt gcgaaagggt gaaacgctcg gttcgcgtgg catcgaagcg accatagact
     3421 cctcgaatcg ctccacaacc gtgggaaagg acgttgatcc ccacgacgat gccaagcagg
     3481 acgaagaggt agatccctgt ttccgttgcg gctttggctc tacgttccat cgccatgatt
     3541 cagctccact tccggctctc gagggtgcgg aaagccatga cgaggcacaa tccggtgatc
     3601 gagaggaagt aaatgacgtt gcgtgtatcg atgagaccac gagcgaattt ctgcaggttg
     3661 gtatcgaagc tcaatacccg aaggaactca ccaatcgcac cgggggcgaa ttccccgatg
     3721 gtggataggt tgtgcagaat gagcaatgcg acgaacgtga tgaagaaggc gacggtttga
     3781 ctttccgtga acgcggaaac catcaggccg atggcaatcg ctgcggcgga aaacaggatc
     3841 aaaccgaggt agccggccaa cacgggacca ggatcgagcg gtccgagatt ccacggccag
     3901 ccaaacatga aaatcggata cagcgcggtg ccaagcaaca gcaccagcac cagtcccaac
     3961 gcccccaaat acttgccgag gatgacttcc gtatctttca ccggcaaagt aatgagcatc
     4021 tcgagcgtgc cagaccgttt ttcctccgcc aacagacgca tggtgatcac ggggatgatg
     4081 aacaggctca gccccagggc tccgtactgg aacatgcggt caaaggaggc acggtcgatc
     4141 tgccagaatc cacctcgaaa caagaagaaa aagattccga gcaggaacaa gcctacgcaa
     4201 atgaccacgt aagcgagcgg tgaataaaag taagaccgaa actctcggcc cgcgatagca
     4261 agcgtgcctt tcatgatttc tcgtcctctt cctcgtccga gtcgtcctcg tcctcgtctt
     4321 gatcctcgtc ctcgtcctcg tcttcttcgt cttcctcgtc ttcttccggc tccgcacgaa
     4381 aaccgcgtgc catacgagac tcggatttgg tcaagtcacg gaatacatcg cccaaagatt
     4441 gagcatcgcg gtgcagttca atgagcaacc agcctttatc gaccatcaat tggaatatgt
     4501 cggatcgcac atcgacatcg cccgcggctt gcaatgtgaa ggtatgggtc gtgtcatcgc
     4561 tgggcgtttc gcgaaccgtg ctgacgccaa caatagagga caatgcgtcc gccacttgct
     4621 gggccgtcgg agccacctga tcaccttggg ccttcttttc atgcacggac accacgtagc
     4681 gagttcttcc gctcttggcc cgaatgtcat cgagcgtgcc atccgccacg acgcgtcctt
     4741 tggatacgat gaccgcgcga gcgcaggcgg attcgacctc ggaaaggtta tgggtggaca
     4801 acagcaccgt gcgttcccta ccgatttcgc gaatgtaatt gatgacttcc gttttctcgt
     4861 tggggtccaa atcactggtg ggctcgtcga gaatcaggat gggcgggtcg tgaatgagcg
     4921 cctgggccaa tccgacgcgc tgacgatacc catgggaaag ggttcggatg tctttgccga
     4981 gaacttgcgc gatgccgcat atttcggcgg atttcttcaa gcgttttttg aattccgagc
     5041 catcgatctc gcgtaccgcc gcgcagaact cgagatagga ccagacggtc atatcgtggt
     5101 acaggggcgc gcgctgaggc agatatccca gctttttccg cacttcgagc ggctgatcga
     5161 agacatcgaa gccatgtatt ttcgcggttc ccgcggttgg agatatgaaa caagtcaaga
     5221 tgcgcatcgt cgtcgatttc ccggcgccgt tagggccaag gaaaccgacc acctcgcccc
     5281 tgcgcacatc gaacgacacc cgatcgagcg cggtgagcga gccgaacatc ttcgtcaagt
     5341 tgtcggcgtg gatcatcaca tcggtggaca tgggtcctcc catgaaggga caagaaacaa
     5401 cgttcagaga gactcgaaaa ctcttggggc agtgtcggtc ccgaggcacc gaaagcacga
     5461 cgccttcggc gcggcggcgg aatctagggg gcaagccgaa tcatgtcaac gttcccgtgc
     5521 gactccattg caaccgtcat gccgcgcccc ggaacattcc agttgccgaa ttattcccac
     5581 ggcgcgatga ttattttgcc aatgcggctt ttcatgctct cgtttggctg gatacccccg
     5641 tcctataggc tcaaccaccc atgggtccgc tgacgaccta cctcatcgaa acgttcgtca
     5701 cgctcgttgc cgtcttggca ctcgcgtttc tcatcctcat gggagcgaaa cgcttcggca
     5761 tcggacaacc catcgggcca atcaccttgg tcggacgcct tccgctcgac acgcgccggg
     5821 ccgtttatct cgtgcgggtg gccaatcaag tcatcatcct gggctcgtcg gaggcgggac
     5881 tttgtaaact cggcgaagtg caagaacaac atatcgagga cttcaaaaac ccccaaacac
     5941 agcctcgttt ctcggacctg atcgctcggt ttcgccaatc atcccgtcgc gtctcccagg
     6001 gctcgaccgc ccccgacaac gaccatggct agccctacct tcgctgaatt ggcccccacg
     6061 agccgtcctt cgcccatcga cgctttcgct gccaagacca atcacgtcgc tcacgcttcg
     6121 acgcgggcat tggcaccgct gctgacgcta cttatcgtgc ttttcagcgc ggggcccgcg
     6181 tggggagccc ctgacggctc ccctgatccg ttgctggcgc gacccatcgc cctcatcatc
     6241 gcactggccc tcgtttcgct tgctcctttc gttttcatga cgctcaccgc gtttttaaaa
     6301 atctcgacgg ttcttcatat tgtgcggggc gctatcggag cgcagaacat tccttccaac
     6361 acggtcgtca tggcccttgc cgcggccttg acgctcatcg cgatggcacc ggtgggtaca
     6421 cgcatcatgc agcgagcggc ccccgtgctt gcggattccg attcggcgaa cacggccacg
     6481 cttgtgcggg ggttagtcga tgcgaccaaa gagccgctgc gtgcgttttt gcgcgccaac
     6541 gcttcgccca cggagcatgg gcgtttttat gaggtcgctc gcgccgcccg gcccccgcaa
     6601 gagcgcgacg acgtggctcg ggacgatttt atcgttctta tccccgcctt catgatcacg
     6661 gaactgatgg aagcgtttgc gctcggattc gtcctttatc ttccgttttt ggtgatcgat
     6721 ctggtcattg ccaacgtctt actgtctttg ggcatgcaga tgctcaatcc cacccaagtc
     6781 agtttgccat tcaagctgtt gctatttgtc gcgattgacg gttgggggct actggcccgt
     6841 gcgttggtga ccggctacgc ggtcgggtga agcgaccggg aacgagggat ttcaggtgga
     6901 gaagaaattg acgcgccccc tttagttgcg aaaaaaatct ggtaggctcc acgcatgcaa
     6961 ggcagcgaat gcatgcttcc ttggcggcca ttgccgcagc atcactctgg aggttcccat
     7021 gaagcgttat cttggatttt taccggtcgg cgcgattgca gtcgcaggat tggttggatt
     7081 gagtattcag gcgggttgcg cgagtgacga ggatccggtc aacaacgcgg gcacgggcgg
     7141 cggcggacag accggcggct cgggcggcgg cgagcaaacc ggcggttcgg gcggtggcga
     7201 aagcggctcg ggcggtggca gccccaccgg aggctcgggt ggcggctcca gccaatgccc
     7261 cgtcggacaa gcagccacgg tggccgatgt cgccgatgcc accacgaccg gcattcaagt
     7321 cgatatcaaa ggcgctatcg ccaccaccaa aaaactcgtg gtgtacttca acaaaaagaa
     7381 aggctcctgc ttgtggggcg tgttcgtgaa agactccgcc gccgatcgtg gcctcatggt
     7441 catcagctac ggcgacaacg ctcccgcgga ctcttcccaa gacaagtgcc caaccggtac
     7501 cgatgatatc cctgatggga tcgtgccggg cgacgttctc gacatcgtgg gtcaaaccga
     7561 cgcttacgcg ccttccacct gcacgggagc tgccaaacaa cgccaactca aggcgtgttc
     7621 ggtaacgaaa accggttcgg gcaatccccc cgcccccgtg gtcgtgacgg acttgaacgc
     7681 gctccacaac ggcgatgcca agtatcaagg tttgttggtg aaaatcgaaa acgtcacggc
     7741 cgagaacttc gacggtggcg cggtcggccc ctacggcgtg atcgaactcg agggcacgga
     7801 cctacagatc aacgacaagt tctactactc cgagtccggc gcaccccaat tcgacccgtc
     7861 ccagcagttc aaacacatca tcggcatcag ccatctcgat tactgtgact gggtgctgca
     7921 acctcgcgac aaatgcactg acttcgatcc tgcctccaaa gactgcatct gatcgccacc
     7981 ttcgtttcac catccccaca accattcgaa acgcaggaaa gtcgtcatat aatgcgcttg
     8041 cagtggctcc tgcgcaaagg tgtgatagcg aagctgcgcc cccaccgccc aggaccgact
     8101 gggacgaaaa tcgagcccaa gcgccgcttg aaatcccaat cgatattccg cttgtcgcaa
     8161 ctcgaatccc gacaactggt atccacccac caacaagccg acgtacggaa cccagctaac
     8221 cacatcaagc gtgtaagcca tgccaagcgc cgcatggctc gcgaccagca tcggctgacc
     8281 tgccactacg gaatgggaag agactcctac ctcgagcaac gcattatacg aatccgtaag
     8341 cccataagca gcgtgcagtc cgccccccaa tccgcttcgc agttcatcgt tccacgacag
     8401 cgccgcgaag cccgcgtcca aacccgcatg ccactgacgt tcgaacgcct ccgcacgcga
     8461 ggcaatcgcc aacagaactg ctaccaacca tgaggtgaat aggagaattc gacgcacccg
     8521 cgcaacctac accaggagcc gcgaacgagc gagggtgtcg aaaaccaatt cgctcgaagt
     8581 gcattcgacg cttgccgaga ctcccgctcg ccgttacctc ctttgtatgc gcgctgtcgt
     8641 atcgaaatgg ctcatcggat ggatggtcgc ttgcgcgggg tgctcttctt cggaagcgga
     8701 ttccaccaac aactatgcga actcgtcgga tggctctttc gtggattcca gttccgactc
     8761 cgccgacgcg agccccgctg tcgatgcagc ggtcgatgcg actggcgaca cgagcgtgga
     8821 caccgttccc gatacggctc aggaccacgc ggcatcagac agtccgtccg aagactcgct
     8881 cctcgacacg accgtggatg cggagcagga tacgccaccg gatgcgcccc cttggatcgt
     8941 aggtaatcag gcgacttgtc cggagcttgc gccgttgcgg gacacgtcta cggatgcttc
     9001 ctcgatcctg cagatgtgta tcgacaggac tccggacggc atgacgttgg tgctcgatcc
     9061 cggacgttac tcgttggcgg ctcaactgca tctggacaga gaaatcggtc tatcaacgac
     9121 cggacgaata ggcacggcgt cctgttctac cgaaccgacc cacggttgcg cggaactctt
     9181 cgcgttgcct gcttttgaca cgcgcttggg gatgtttctt gccacagggg cagtcaccat
     9241 cgaccacatc gtgctgcatg gtaaccgaca aggacgctcg gggacccatg cccatcagat
     9301 gtgttcggca ctgacggaca acgcgtatgg ctacaacgcg acgctacaat gttcgaactg
     9361 tcgcatgacc catagcgtct cgatgtatgc gctttgcgga acgggccttc aagtcatcgc
     9421 accggccgtg aacgtgacca tttctcataa caccttcgcg ttcaacggat tgcataccac
     9481 gaaaaatatg tggtccgatg gtctcacggt ccatgactcc gaaaaaggcg tgtatgagca
     9541 caacacgttc atcgataaca cggatatcga ccttattttc ggtggctgta aaaaatgtaa
     9601 gattcggtat aaccaggtcc atcacacatc cgatccggcg gggggcgctt tcgccgcgat
     9661 catgatccag aaatggccga cgacatccgg ctgttatgag gacgtcgaca tttccggcaa
     9721 cgcggtggat tgcgggcctc ttcgcgcctg cggctccgcg ttttacatcg gctccgagtc
     9781 ctggtacccg gaaactcctt acggcacact gcaaaatggt acgaccagcg ggttgatcaa
     9841 aggcaattcc gtcaatcgtg cgatgaacgc cttttacatc gcggcccgag gccttgcgat
     9901 ctacggaaat gattttttgc aggctcacgg cgtgccgatt cccaacagtt gtcacaaaaa
     9961 catcgtgagc acgacaccga tcgtggtttc acccacggca gcgagttgtc acttcaactt
    10021 cgaaaacgtg gatccgggta tgtccattca ttacagcagc gcgagttggg cggggtgtgt
    10081 cccgaattac ccattttgac tcgttggaac ggcgccttct tcgactacga gcaaacgctt
    10141 tcatggtcac catggtatgg gcacaaaggg ttcgaaccga ttggcggcga aaagcggctc
    10201 tgatcgatcg atagcgtggt gcaaaatcgg cacggctcgt gtcgcggtat cgagcggcaa
    10261 tctcggagac gaggctgttt tccgttgaag gtatggttta tggttcgcca ggcgagtgcg
    10321 gcgcgggcct gggattcgac attttttgtt tattgcgcag ctttcaccag gggttgttca
    10381 ggcggaaaga ccggttcgag gttccagatg tcacgggcat actcacgaat ggtgcgatcg
    10441 ctggagaact tgcctacgcg tgcggtattg gtgatggcca tgcgcgccca tcgcgccttg
    10501 tctcgatacg ccttgtccac ctcatcctgc gtttgacaat aggcttcata atccgcggcc
    10561 acgaaaaatg ggtcgccatg cacgaggatg ctatcgacca ccggcgacaa ctcccccgcc
    10621 ggcgcaaata catcggaatg cagccagtcg agcaccgctt tcaactccgg gttgcgttga
    10681 taaatcagcc gcgcatcgta gccagaacgt cgaagctcat cgatctgctg cacggtattg
    10741 ccaaaaatga aaatgttatc gctgccgact tcttcgagga tttccacgtt ggctccatcg
    10801 agcgtgccga tcgtcacggc cccgttgaga gcgagtttca tgttgcctgt gccggaagcc
    10861 tccttgcctg ccgtagaaat ctgctgagac acatcggtgg cgggaatgat tttctccgca
    10921 aggctcaccg aataattggg aagaaaagcg atcttgatct tgtcttgaat gcgcggatcg
    10981 tcgttgattt tggcggctac ggcgttgatg gcccggatga tccgtttggc aagatcatag
    11041 ccgggcgccg ccttcgcgcc gaagatgaaa acgcgtttgt tgatcggaag atccggatcg
    11101 tgcagcaacc gacggtacag ggtcaggata taaagcagat tcaggtgctg acgtttgtat
    11161 tcatgcaacc gcttgacttg cacgtcgaag atggcattgg ggtcgaccac aaggccgcaa
    11221 gaagccttga tttcctcggt taaatcaatt ttattggcgt gtttgaccgc catgaaggct
    11281 ttctgaaatt cgggatcgtt ggcgtggtct tgcagggggc gaagccg
//
LOCUS       DAOSOR010000128        11262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5750_length_11262_cov_4.077184, whole genome shotgun sequence.
ACCESSION   DAOSOR010000128 DAOSOR010000000
VERSION     DAOSOR010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11262
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5750_length_11262_cov_4.077184"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="PK710_09075"
     CDS             <1..1093
                     /locus_tag="PK710_09075"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732583.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOT09910.1"
                     /translation="SKPGFSLLEHLGFSQEQIAQANDVIIGRMTIEGAPHIDHSHLPV
                     FDCANRCGRHGQRFLSASAHLHMMAAVQPFLSGAISKTVNVPNEATVDDIKELYEEGW
                     RIGLKAVAIYRDGCKASQPLSSGSPTTTKEHQSTGPEPVASDDIHTPAVRTPGVRIRL
                     PKKRTGFTQEAQVGGHKVFLRTGEYADGTLGEIFIDMHKEGAAFRSIMNCLSMSVSIG
                     LQYGVPLETYVDQFTFTRFEPHGPVIGHTNVKFATSIVDYLFRVLGIEYLHRYDLAHV
                     PPADAAAIQDPTETKNQDPLTLPPPEQGVLPLAAPLPFLSAGVEHPQAQHLSSMMGDA
                     PICYVCGHTTVRNGSCYRCLNCGHSLGCS"
     gene            complement(1300..1671)
                     /gene="rbfA"
                     /locus_tag="PK710_09080"
     CDS             complement(1300..1671)
                     /gene="rbfA"
                     /locus_tag="PK710_09080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOT09911.1"
                     /translation="MMPGEGWRSARVADRVREELARVLTREVSDPRLEGLRVLRVEIS
                     GDLQIAWVYLGLGHLDSPEQRSRALRGVASAQNRIRKRISSRLGLRRAPELRFAFDEA
                     IDEQSRILALLDEVEREGVDR"
     gene            complement(1658..2044)
                     /locus_tag="PK710_09085"
     CDS             complement(1658..2044)
                     /locus_tag="PK710_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOT09912.1"
                     /translation="MFVGVQRLVLQIPGARSLKDRRQVVRSFKDRVQARLRISVAEIG
                     HLDRPSHAVVAMAVVSNEAAVCDEILAKAAMMASTLGNAVLADRATEIVPFGFAGSGI
                     RGGIEQLRLAEEEMWRDIDEEQDDAR"
     gene            complement(2102..5386)
                     /gene="infB"
                     /locus_tag="PK710_09090"
     CDS             complement(2102..5386)
                     /gene="infB"
                     /locus_tag="PK710_09090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOT09913.1"
                     /translation="MSKVRVYEVARDLNVDQDHLVGVLQSLGFSDVRNRMSKVDADAV
                     ERVRRHIESQQRTPEVVQERLSDTVIRRRRVGAPTRPSSSAAPVGTAPVVEMEKRPDP
                     PVRKRKVAEKAVAPPTDAASEKAPDLVEKPAVPVEPVSDSVAETASGSGVDPTVDGSA
                     EPVIEEVVESSAVKTAETPVDESVEPSFDKPLETATAPSVITELPATVTKQAVEKEQG
                     EHVSEAASQEPEAVAPVVAPDKEPVAQPGPSVVEHIVEPTPLTLDTPRDIAVAAWLAG
                     EPLVGEPVKASKEAAPAVVEPSKTVEAAPESAVSEKTTVEPREEISAAVQDTVSALTS
                     EVPDVGLVQERSDIVPSVSKESEEPFVERSEERESHRELAGEDESAGEEPVVTTKLSQ
                     DAPTRVPVRPASPTRVAPPVPQPPVRTQQHRTGIDVWEGRPGVPMRQPPQPRPSTTPA
                     SPVRRREYDPRAASPATKTETRTTANRRTWAAGRGKRGVAAPVPSKKPVATKEMSEHK
                     KIIKIEAQVSLQALAARMSLKATEVLMKLMSMGLPGVNINSTLDADTAKLVASEFGWE
                     VEDVAVSEEEALNLARDEADEDTANLELRPPIVTVMGHVDHGKTSLLDQIRKTNLVAA
                     EAGGITQHIGAYRVNTSRGFITFLDTPGHAAFTQMRLRGAQVTDIVILVVAADDGVMP
                     QTKEALSHAMSAQVPIIVAVNKIDKESANPERIRREMSDLGLVPEEWGGETIFCDVSA
                     KTREGLDALLEMTALQAELLELRANPKKPAVGTVIEALLDRGKGPVARVVIMDGTLNT
                     GDVILAGTSWGKVRAMTDDRGRAVRSAGPSMPVEILGLSEVPSAGDPIHVVKGVKIAQ
                     DLAQQRKTKASTSLIPAGAKVSLEDLTKRIAEADQLELRIIVKADVQGSVEAVADALS
                     GLSTEKVRVAIIYAGVGGITEGDVNLAVASKAIIVGFNVRPAGKSGQLAELEGIEIRL
                     YSVIYNAIDDVKNAMLGLVGPSYEERQLGKAEVREIFHIAKIGTVAGCFVQEGLLRRN
                     AKLRVIRDSVQIFEGRFASLKRFKDDAREVDKGFECGLSIESYNDIKVGDIIECFEVA
                     EVQATL"
     gene            complement(5383..6003)
                     /locus_tag="PK710_09095"
     CDS             complement(5383..6003)
                     /locus_tag="PK710_09095"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF448 domain-containing protein"
                     /protein_id="HOT09914.1"
                     /translation="MAVPQTQDRHCRAPRRAPERTCVGCGKRDAAPNLLRIVLGPEGH
                     VAFDLANHAMGRGAHVHPAPQCMDSAARKGLSKAFHTRVVCEPIALRDMLAGAVERRL
                     KGLLGSAIQSGNAVLGRQAVDDELGKNTIETVILACDASDAPGPALKRAIADGRAVAW
                     GSKGQLGAALGRSEVGVVGIRSPSLGRAIADMCRIADGVRSCSEVR"
     gene            complement(6009..7676)
                     /gene="nusA"
                     /locus_tag="PK710_09100"
     CDS             complement(6009..7676)
                     /gene="nusA"
                     /locus_tag="PK710_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOT09915.1"
                     /translation="MAAPSYASGSEPNLGLVIEQVAKEKGIEKHVLIESIEAAIHKAA
                     QSAFGAARELEAKFNEETGQVDLYQYMTVVEVVTEPERELSLEDAKRYNLVAEVGEEL
                     GFQVFWRSEDEERARRQDEQFGDLLKLKQASATFGRIAAQAAKQVLLQRVRDAERDII
                     YDEYKDRKGELIRGIVRRFEKGNNIIVDLGRTEGVLPYREQTPRETYRHGDRIVAYVK
                     EIDPQAKGSQIVLSRSDPKLVEKLFESEVPEIYEGIVRIVGVAREPGSRSKIAVTSRD
                     ADVDPVGACVGMKGSRVQAVVQELRGEKIDIVPYDRDAARFVIAAIQPAEVTKVIVDE
                     ADGRMELVVPDEKLSLAIGKKGQNVRLAAGLTKWKLDIISEAKFKQIEEEAMTALQQI
                     DQVSESVAKSMYRLGFRSLEEVAEAGTDELGAIPGLGGQDVALLIKQSAEETMERLRQ
                     ERIRAAAQRTEPLTDRERLLFIRGVGQRTVTLLEEAGYRTVEDVLREDIDRLAIRTGL
                     GIKKARLIKQGAQYFVESEQKVLDDARQEAKRVAEDAASQPPKQQEH"
     gene            complement(7690..8220)
                     /gene="rimP"
                     /locus_tag="PK710_09105"
     CDS             complement(7690..8220)
                     /gene="rimP"
                     /locus_tag="PK710_09105"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOT09916.1"
                     /translation="MLGEVEAVVCPVLQAHGLDLFDLSFRVEKGGWVLRVTIEPPVES
                     VERGVTLEQCAEVSRDISTALDVADPLAHAYTLEVSSPGVERPLRTLAHFSRFVGKPA
                     KVTMRMPVEGMGMVLRGVIAGVDGESVLLDVGLPNPVVLALSDIKRANLVYELPSHPK
                     KGNSKKKRRSGEERRD"
     gene            complement(8519..10984)
                     /locus_tag="PK710_09110"
     CDS             complement(8519..10984)
                     /locus_tag="PK710_09110"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT09917.1"
                     /translation="MKRAFLGVLMGALSLYSSTGLAFIWPNVPQEIAQGLQSEDEGER
                     RAAAERLHELPQSIAVSHVLTALDDSDAEVRLAASRVAVEMRVQGSGERVIDWLGERD
                     VRLRLMACEVLRVSPSSRALAALSRVLGDSNAEVRVAAALTLGTIGDREAVAALLGHL
                     DDASVEVRTAMIEALGRIGDHRAVVPLLGKVQDNAVEVRRMTVRVLGDLGDQRASSAL
                     IVALRDKANEVRREAIESLGRLRANDAVSALAPLIQERGSLMLRETAIEALGRIGGDV
                     AIEALLKALEQDEPTSEESVVRRALGRGGPSMIPRLVSTMQQSSHPNVVSGCALVLGE
                     LGSEEQIPLIVGEMQRGKVSERVGLAALAKMRSVKALPFVLEMLDGATPDTRRAAMLV
                     AEQLLNPSDRDGRFVDPLLSRLMDSKLDLRERELLIRLLGKTGSPRAVTALSEFAVST
                     QPRLKRAAIDALGEIGPAGQDAELLTALDDTEPSIRFAAAYALSRVSAPATAGVLYRR
                     LMHASEQDRLALGIALSGAMQRATDDRLVTEVVEGLDRVRPPLRDVLVEGLGRMRTRS
                     AGEALMGLARSPWVDDRRKVAEALGGHAERVDVVRQLLSDADPSVQANAAWSLGKIGA
                     REDAARLQALVTHRDAAVASNAVGALGLFWGRGLVRSSEPFCAALGDSRAYVRVNALA
                     ALRLANARCGDGSVERQILNSDASVMVRIAAARLLSAVPSTIPEADERGLRRCLLDDR
                     SGQVAHVCHRAGIERRGDTTDEIIVYVVPDGRQEPMGRASYALVFETGSMRLGLADRR
                     GAVYESMAPRGKVELAVPAIRLR"
BASE COUNT         2374 a         3696 c         2744 g         2448 t
ORIGIN      
        1 ttcgaaacca ggcttttccc tgctcgaaca cctcggtttt tcccaagaac aaatcgcaca
       61 agccaatgac gtcatcattg gacgaatgac catcgaaggc gcgccgcaca tcgatcattc
      121 tcaccttccg gttttcgatt gcgccaatcg ctgcgggcgc catggccaac ggtttttatc
      181 cgcctctgcc cacctgcaca tgatggccgc cgttcaaccc ttcctttccg gagccatctc
      241 caaaaccgtc aacgtcccca acgaggcaac cgtagacgat atcaaagagt tgtatgaaga
      301 aggttggcgc attggcctga aggctgtcgc catttatcgc gatggctgca aagcctccca
      361 acccctgtcc tcgggctcac caaccacaac caaagaacat caatcaaccg gcccagagcc
      421 cgtcgcttcc gacgatatcc acacgcctgc cgtccgcact cccggtgttc gcattcggtt
      481 gcccaaaaaa cgaactggtt tcacgcaaga agcgcaagtc ggtggacata aggtcttttt
      541 acgaaccgga gaatacgccg acggcaccct tggtgagatt ttcatcgata tgcataaaga
      601 aggggccgcg tttcggtcca tcatgaactg cttatccatg tccgtgtcga taggcttgca
      661 atatggggtt cctctcgaaa cctacgtcga ccaattcacg ttcacccgtt tcgagcccca
      721 tggtcccgtc attggacaca cgaacgtgaa gtttgccact tcgatcgtgg attatctatt
      781 tcgggtgctg gggatcgaat acttgcatcg gtacgatctt gcgcatgttc ccccggccga
      841 cgccgctgcc attcaagatc ccacggaaac gaaaaaccaa gatcccctta ccttgccacc
      901 accggaacag ggggtccttc ctctggctgc tcccctgccc tttctatccg caggagtcga
      961 acacccccaa gctcaacacc tcagttcgat gatgggggat gcgccgattt gttatgtctg
     1021 cggtcatacc accgtacgaa acggttcgtg ctaccgatgc ttgaactgcg gacactccct
     1081 cggctgctcc tgacaccctt tcgccgggcg gcaagcctgt aactatttgt atttattact
     1141 tattcctcgc cgcgaacgca gacttgccat cccgagagta tcgataagca tgggttagcg
     1201 gtgccacggt cgtcgtcatc agttccgctt ccgttcatca cgccaagccg ttcgcccgcc
     1261 ccaccctccg cgctccaccc cttcggccat gccgcggatt catcggtcga cgccttctcg
     1321 ctcaacctcg tcgagcaaag ccaaaatccg ggattgctcg tcgatggctt cgtcaaaagc
     1381 gaaacgcaat tctggagcac ggcgaagacc gagtcgtgag gagattcgtt ttcgaattct
     1441 gttttgagcc gatgccacgc cacgcaaagc gcggcttcgc tgctcggggc tatcgagatg
     1501 gccaagccca aggtagaccc aagcgatttg tagatctccg gagatctcta cccgaaggac
     1561 acgaagccct tcgagtcggg ggtcggatac ttctcgtgtc aacacccgtg cgagttcctc
     1621 tcgaacgcgg tcagccacgc gggcgcttcg ccatccttca cctggcatca tcctgctcct
     1681 cgtcgatatc ccgccacatt tcttcttctg ctaaccgaag ttgttcaatt ccaccgcgaa
     1741 ttccggatcc tgcaaaacca aagggaacga tctcggtggc acggtccgcc aatacggcgt
     1801 ttcccagggt cgatgccatc atcgctgctt ttgcgaggat ttcgtcgcat acggccgctt
     1861 cgttggatac gactgccatg gccacgacgg catgggaggg acggtcgaga tggcctattt
     1921 ccgcaacgga tattcgcaat cgggcttgaa cacgatcttt gaagctacgc acgacttgac
     1981 gacggtcttt gagagatcga gcgccaggga tctgaagcac gaggcgttgt acacctacga
     2041 acatggcaat gattccaagg gattagggaa cggcggggca gcagcgggcc gtttgggatt
     2101 gtcagagcgt agcctggacc tcggcgactt caaagcactc gatgatgtca cccaccttga
     2161 tgtcattgta gctttcaatg gaaaggccgc attcgaagcc tttgtcgact tctcgcgcat
     2221 catctttgaa tcgcttcaag ctggcgaagc gtccttcgaa aatctggaca ctgtcgcgaa
     2281 tgacacgaag cttggcgtta cgtcgaagga ggccctcttg cacgaaacag ccagccacgg
     2341 ttccgatttt cgcaatatga aagatctctc gaacctcggc ttttccgagc tgccgttcct
     2401 cgtacgacgg tcccaccagg ccaagcatgg cgtttttgac atcgtcgata gcgttataga
     2461 tgacgctata caggcggatt tcaatacctt ctagttctgc cagttgcccg gatttgcctg
     2521 ccgggcgcac gttgaatccg acgatgatgg ctttggatgc cacggcgagg ttgacatcgc
     2581 cttcggtaat gccgcctaca ccggcataaa tgatggcgac tcggactttt tcggtcgata
     2641 ggccggataa ggcatcggca accgcttcga cagatccttg tacgtcggct ttgacgatga
     2701 tgcgaagttc gagttgatcg gcttcggcga tacgtttggt gaggtcttcg agggagactt
     2761 tggcgccggc aggaatcaag ctagtggatg ctttcgtttt tcgttgttga gccagatcct
     2821 gagcaatttt cacgccttta acgacatgga tgggatcgcc agcgcttggt acttcggaca
     2881 atccaaggat ctctacgggc atcgaagggc cggcggatcg gacagcgcgg ccccgatcgt
     2941 cggtcattgc cctgactttt ccccacgagg taccggcaag gatgacatct ccggtgttga
     3001 gtgttccatc catgatgacc acacgggcga caggtccttt gcctcggtcc aacaacgcct
     3061 cgatgacggt gccaacggcc ggttttttgg gattggcgcg aagctctagc aattctgctt
     3121 gcagcgcggt catttccaaa agcgcatcca atccttctct tgttttggcc gacacatcgc
     3181 agaagatggt ttctccaccc cattcttccg gcacaagccc caagtccgac atttccctac
     3241 gaattcgctc aggatttgcc gattctttat cgattttgtt cacggccacg atgatgggaa
     3301 cttgagctga catggcgtgg ctcaacgcct ctttggtttg aggcatgacc ccatcatccg
     3361 ccgcgacaac caagatgacg atgtccgtga cttgggctcc ccgcagacgc atttgtgtga
     3421 atgcggcgtg cccaggcgtg tcgaggaatg tgatgaaccc gcgagaggta ttgacgcggt
     3481 acgcaccgat atgctgcgta atgcctcctg cctcggccgc taccagattc gtttttcgga
     3541 tctgatcgag gagtgaggtt ttcccgtggt caacgtgtcc catgacggtc acgatgggcg
     3601 ggcgcaactc gagattggcg gtatcctcat cagcctcatc gcgcgccaaa ttcagcgctt
     3661 cttcctcgct taccgccaca tcttcgacct cccagccaaa ctcactcgcc acgagtttgg
     3721 ccgtatctgc atcaagggtc gaattgatgt tcacccccgg caaccccatc gacatcaatt
     3781 tcatgagcac ttcggttgct ttcaggctca tgcgagctgc caaggcttga agcgatacct
     3841 gagcctcaat cttgatgatt ttcttatgct cagacatttc tttcgtagct acgggtttct
     3901 tggacggaac gggcgcagcc accccgcgct tcccacgtcc tgctgcccaa gtgcgacgat
     3961 tcgctgtcgt tcgggtttcg gttttggtag ctggactggc cgcccttggg tcgtattcac
     4021 gtcttcgaac agggctcgca ggtgtcgtag aaggccgagg ttggggtggt tgacgcatgg
     4081 gcactcccgg acgcccttcc caaacgtcga tgccagttcg gtgttgctgc gttcgcaccg
     4141 ggggctgcgg gactggaggt gccacgcgtg tcggcgatgc gggcctgacg gggacacgcg
     4201 tgggggcgtc ctgcgaaagc ttcgttgtca ctacaggctc ctctcccgca ctctcatctt
     4261 ctcccgccaa ctcacgatga ctctcgcgtt cttccgatcg ctcgacaaag ggttcctccg
     4321 actccttaga aacgctcggt acgatatcgg aacgttcctg gaccaatccc acgtctggca
     4381 cctccgaagt gagtgccgaa acagtatctt gaacagccgc cgatatttct tcacggggct
     4441 ctacggtcgt cttttcggaa accgcggatt caggcgcggc ctcaacggtt ttactgggct
     4501 ccaccaccgc aggcgcggct tctttggacg ccttgacagg ctcgcctacc aaaggctccc
     4561 ccgccaacca cgcggccacc gcgatgtccc tcggtgtatc aagagtcaag ggcgtcggct
     4621 ccacaatatg ctcgactacc gaaggccccg gttgggctac cggttctttg tcaggagcga
     4681 cgacaggagc aacggcttcg ggctcttgcg acgcggcctc cgaaacatgc tccccttgct
     4741 ccttctccac ggcttgcttg gtcactgtcg caggcaactc cgtgataacc gatggcgctg
     4801 tggctgtctc gagcggcttg tcgaacgaag gttccacgga ttcatcaacc ggcgtttccg
     4861 ctgttttaac ggcagacgat tccacgactt cttcgattac cggttcagcc gacccgtcca
     4921 ccgtcgggtc gacacctgaa ccgctcgcgg tctcggcaac cgaatcgctg acgggttcaa
     4981 caggcactgc cggtttttca acgagatccg gtgccttttc ggaagcggca tcggtggggg
     5041 gagcgacagc tttttcggcg acttttcgct tccgcaccgg cggatcaggc cgcttttcca
     5101 tttccaccac gggagcggta cccactggag cagccgacga agaaggccgt gttggcgcac
     5161 ccactcgccg tcgcctgatg accgtgtcac tcagacgctc ttgcaccacc tcgggcgttc
     5221 gctgctggct ttcgatgtgg cgccgaacgc gctcgaccgc atccgcatcg actttgctca
     5281 tacggttgcg cacatcgctg aaacccagag actgtagaac accgaccaag tggtcctggt
     5341 ccacgttcag gtctcgcgca acctcgtaca cccgaacctt gctcatcgaa cctccgaaca
     5401 agaccgcaca ccgtccgcga tacgacacat atcagcaatt gctctcccca aagaaggaga
     5461 acgaatcccg acgacaccga cttcacttcg tcccaacgcg gcgcccaatt ggcctttcga
     5521 accccaggcc acggcgcggc catctgcgat tgctcgcttc aaggctgggc caggggcgtc
     5581 gcttgcatca caggcgagga tgactgtctc aatcgtgttt ttcccgagtt catcatccac
     5641 ggcctgccta ccgagcacgg cgttaccaga ttggatcgcc gagcccagaa gacctttcaa
     5701 tcgtctttcc accgcacccg cgagcatgtc gcgaagcgca ataggctcac acaccacgcg
     5761 ggtgtgaaac gctttcgaca aaccttttcg tgcggcacta tccatacact gcggcgcggg
     5821 atgcacgtgc gccccacgcc ccatggcatg atttgccaga tcaaaagcca cgtgtccttc
     5881 cggtcccaac acaatccgaa ggagattcgg ggcagcatca cgtttaccgc aacccacaca
     5941 cgttcgctcc ggcgcacgac gaggcgcccg gcagtgacga tcctgggttt gcggaacggc
     6001 catggtgatt agtgctcctg ctgcttcggg ggttgcgacg ccgcatcttc ggcaacacgc
     6061 ttggcttctt gccgcgcatc atcaagcact ttttgttcgc tctccacgaa gtactgcgca
     6121 ccttgcttga tgagccgcgc ttttttgatc ccgagacccg ttcgaatggc aagccggtca
     6181 atatcttctc gaagcacgtc ttccactgtc cgatacccag cctcttcaag aagagtcacc
     6241 gtccgttgcc caaccccacg gatgaacaac agtctttccc gatccgtcaa gggttcggtt
     6301 cgttgtgcgg ccgcgcggat acgctcctgt cggagccgtt ccatcgtctc ttccgcactt
     6361 tgcttgatca gcagcgccac gtcttgtccg cccaagccgg gaatagcgcc gagttcatcg
     6421 gtcccagctt ccgccacctc ctccagagat cggaagccca atcgatacat cgatttagca
     6481 acgctttccg agacctgatc gatttgttgg agagccgtca tggcttcttc ttcgatctgc
     6541 ttgaacttgg cttcgctaat gatgtccaat ttccacttcg tcagtccggc cgccagacga
     6601 acgttctgac ctttctttcc gatggctaac gatagttttt cgtcgggtac cacgagttcc
     6661 attcgcccat cggcttcatc cacgatgact ttcgtcacct cggccggttg aatggccgcg
     6721 atcacgaatc gcgcagcatc ccggtcataa ggcacgatgt cgatcttttc ccctcgcaat
     6781 tcctgcacga ccgcctgcac ccgtgagccc ttcatgccaa cgcatgcacc taccggatcg
     6841 acatccgcat ctcgggacgt caccgcaatt ttcgagcgag accccggttc ccgtgccact
     6901 cccacaattc gaacgatccc ctcgtagatt tcaggaacct cggattcaaa aagcttctcc
     6961 accagctttg ggtcagatcg cgacaatacg atctgagaac ccttggcttg cggatcgatc
     7021 tctttgacat aagcgacaat acggtcgccg tggcgatagg tctcacgagg cgtctgctct
     7081 cgatacggaa gcaccccctc ggttcgaccg agatcgacaa tgatattgtt tcctttttcg
     7141 aagcgacgaa cgattccgcg gatgagttcc ccttttcggt ctttgtactc gtcataaata
     7201 atgtctcgct cagcatcgcg aacacgctgc aggagtacct gtttggcagc ttgagccgca
     7261 atgcgaccga aggtggcgct cgcttgcttc aacttcaata agtcaccgaa ttgttcatct
     7321 tgccttcggg cccgttcctc atcctccgag cgccaaaata cttgaaatcc aagctcttcg
     7381 cccacttccg cgaccagatt gtatcgcttg gcatcctcca acgacaactc tcgctcgggc
     7441 tccgtcacca cttctacgac ggtcatgtat tggtacaaat cgacttgtcc cgtttcctcg
     7501 ttgaacttcg cttcgagttc tcgcgccgca ccgaatgcgc tctgcgccgc tttgtgaatc
     7561 gcggcctcga tcgactcaat caacacatgc ttttcaatgc ctttttcttt ggcaacttgc
     7621 tctatcacca acccgagatt gggctctgaa ccagaagcat aggaaggggc tgccatgatt
     7681 cgtgctccgt caatctcgtc gttcttcacc agaacgacgc ttcttcttcg aattgccctt
     7741 ctttggatga gaaggcagct cataaacaag gttcgcgcgc ttgatgtcag acaacgccaa
     7801 aacaacagga ttcggcagtc caacgtctag caataccgat tcaccgtcca cacctgcaat
     7861 cacacctcgc agcaccatgc ccataccctc caccggcatt cgcatggtta ccttcgctgg
     7921 cttgcccacg aatcgcgaaa aatgagcaag cgttcgaagc ggccgctcca ccccaggaga
     7981 agaaacctcc agcgtatacg catgagcaag cggatcggct acatccaacg ctgtcgagat
     8041 gtcccgcgat acctctgcgc attgctccaa cgtcacccct cgctccaccg attcaacagg
     8101 gggctcgatc gtgacgcgaa ggacccaccc ccccttttcc acacgaaaac tcaggtcgaa
     8161 taaatccaaa ccgtgcgcct gaagcaccgg acaaaccacg gcttcgacct cgccaagcaa
     8221 ggcggcctct ttgttctttg tcctgtcctt cgattcgacc aagtttctcc tatcggattg
     8281 caccaattgc ccgctggcta cctcataccg aagtgggcaa agaaagaacc gcgggagcga
     8341 aacaaaaaaa agcggactct cgtaggggag cccgcgacgg tccacccggt aattgcggcc
     8401 ggacgatagc ctcaggcctt gccttgtgca actggaacct tcaggaagtg tcaagtcatg
     8461 gcgggtttcg ctttgcacga cttataaaat aagtcaataa atacaatata ttacataatt
     8521 atcgaagtcg tatcgcaggc actgccagct ccaccttccc ccttggcgcc atgctttcat
     8581 ataccgctcc tcgacggtcc gctaacccca aacgcatact gcctgtctcg aatacaagcg
     8641 cataggacgc tcgacccatc ggctcctgcc gaccgtccgg cacaacatac acaattatct
     8701 catccgtagt gtctcccctg cgctcgatcc cggctcgatg acaaacatgc gccacctgtc
     8761 cgcttcgatc atccaacaaa caacgacgca atcctcgttc gtcggcctcg ggaatcgtcg
     8821 atggcacggc cgataacagc cgcgccgccg caattcgaac catcaccgac gcatccgaat
     8881 tgaggatttg cctttccacc gatccatccc cgcatcttgc attcgctaac cgcaacgccg
     8941 ccaacgcatt cacacggaca tacgctcgcg aatcaccaag cgccgcacag aaaggttccg
     9001 acgagcgaac cagcccccgc ccccaaaaca accccaaggc ccccaccgca ttgcttgcca
     9061 ccgcggcatc tcgatgcgta accaatgcct gcaatcgcgc tgcatcctca cgagcaccaa
     9121 tcttgcccaa cgaccatgca gcattggctt gcacggatgg atccgcatcg ctcaacaact
     9181 gccgaaccac gtccactctc tccgcatgcc cccccaaagc ctccgccacc tttcgacgat
     9241 cgtccaccca aggactgcgt gccagaccca tcaaggcttc acccgctgac cgggttcgca
     9301 tccgtcccaa tccctccacg agaacgtcac gcagcggcgg ccgtacccga tccaatccct
     9361 ccactacctc cgtcaccaaa cggtcatcag tcgctcgttg catcgctccc gacaacgcaa
     9421 tccctaacgc caatcgatcc tgttccgatg catgcatcag ccttcgatac aagacacctg
     9481 cggttgccgg agcagacact cgcgacaaag catacgccgc cgcaaaccga atcgacggct
     9541 ccgtgtcgtc gagtgccgta agcaattccg catcctgtcc cgcgggacca atctccccca
     9601 acgcatcaat cgccgctcgt ttcaaacgcg gttgcgtcga caccgcaaac tcggacaacg
     9661 ccgtgaccgc acggggtgaa cccgtctttc ccaacaaccg aatcaacagc tctcgctcac
     9721 gaagatcgag cttcgaatcc atcaacctac tcaacagcgg atctacgaat cgtccatccc
     9781 gatccgatgg attcaatagt tgttccgcta ccagcatggc ggctcttcgc gtgtccgggg
     9841 ttgcgccatc gagcatttcc aacacgaaag gaagcgcttt gacactcctc attttcgcca
     9901 atgccgctag ccccacgcgc tccgatacct tgcctcgttg catctcccct acgatcaacg
     9961 gtatctgctc ctccgatccc aactcgccca acaccaaagc gcaacccgat accacgttcg
    10021 gatgcgatga ttgctgcatc gtggacacga gccgaggaat catggaagga ccaccacgcc
    10081 ccaacgctcg tcgaaccaca gactcctccg atgtcggctc atcctgctcg agagctttca
    10141 acaatgcttc gatcgcaacg tctccgccta tcctccccaa cgcctcgatc gccgtttccc
    10201 gaagcatcaa cgaaccacgc tcctgaataa gcggcgccaa agcgcttacc gcatcattgg
    10261 cacgaagcct gcccaatgat tcgatagcct cgcgccgcac ctcgttggct ttgtcccgca
    10321 gggcaacaat gagcgcactg gaagctcgct gatcaccaag atccccaaga acacgaaccg
    10381 tcattcgacg tacttcgacc gcgttgtcct gcaccttccc aagcaaaggc accacggcac
    10441 gatggtctcc aattcttccc aacgcttcga tcatcgcggt ccggacttcg acgctcgcgt
    10501 catccaagtg cccgagcaag gcagccacgg cttcgcgatc cccaatcgtc ccaagcgtca
    10561 atgccgccgc cactcgaacc tccgcattcg agtctcccaa cacccgagac aaagctgcca
    10621 aggcacgcga tgaaggcgat acccgcagca cttcgcaggc catgagacga agtcgtacat
    10681 ccctttcccc caaccaatcg atcacgcgtt caccagatcc ttgcacccgc atctcgaccg
    10741 caacacgcga agcagcaagc cgcacctccg catcggaatc atcaagcgcc gtcaacacat
    10801 gcgatactgc aatgctctga ggcaactcgt gcagtctttc cgccgccgca cgacgctccc
    10861 cttcgtcttc cgactgtagc ccttgcgcga tttcttgtgg aacattcggc caaatgaagg
    10921 ctaggccagt agaggagtac agcgataacg cgcccatcaa gacgcccaag aacgctcgtt
    10981 tcacacggtg aagcctacca taccttcacc cctcgcacgc aagcccccaa caagctcgtg
    11041 ctgaaccctg gaagataacg aacgcaccgt cacattggat gtacattcac acgcatcgcc
    11101 gacgacgctc catccaacaa cgacaagcgc atgaccgtcg accgggccgg ctttgccacc
    11161 gccgtggacc gcgtgtcgac catcgcgtcg gaccggggcg gcaaggcggt gaagcttcac
    11221 ctctcggaag gccagctcga actcagcgtc accaatcccg ac
//
LOCUS       DAOSOR010000129        11208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5788_length_11208_cov_4.145342, whole genome shotgun sequence.
ACCESSION   DAOSOR010000129 DAOSOR010000000
VERSION     DAOSOR010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11208
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5788_length_11208_cov_4.145342"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="PK710_09115"
     CDS             complement(<1..1141)
                     /locus_tag="PK710_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09918.1"
                     /translation="MMRHIRLGALVVAVLAGGCFPPQQPAQEPSGSAIPEESKAMVRQ
                     GDEMVLAAQKQMDEEIDKNQAEIEKAMKAAVGQEASFAGGPMPMPASQAIKEVRAAKI
                     ELSISPVLDAQGKPVANQFMQLEDSYSKRAQEIGPKMASKKATQAEIKFLQDGVQHVQ
                     KINDLKAQVRLATTPAMNAGWMVTTGSMTTMTTVAGMIRSRRQMEMQWTDQDYEMVRQ
                     LMERQSRREAVAALSMALMATYQAVVNHGADPSLIDDVAKAGIEAIPLQGQASLEDAK
                     TYVDHFAQNVEASQKTYEEQMRKTFGDQEYEAKYKAQLDGMFAQAASATKALSATELM
                     AQTNEKYKADLEKCARGEALPPDTMVGPAKCKEAKASANPDGSLSP"
     gene            complement(1162..3282)
                     /locus_tag="PK710_09120"
     CDS             complement(1162..3282)
                     /locus_tag="PK710_09120"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOT09919.1"
                     /translation="MKTFATPFLCLLVAACGGSSPALTPLPSPAPSTSASAVTGPSTP
                     MTSIPRQDTSLIPRKVLFGDPDRARVRLSHDGKWLSFLAPKDGVLNLWVAPALDHRNA
                     RPLTSETERSITRYQWAYDNKHIVFSKDKGGDENWYLIALDVESGAQRVLSPDKDVQA
                     NLLHASPQHPREVLVGLNDRDPKWHDVYRIDVTTGKRQLVYKNEERFSGFLADDGFRL
                     RVGIKQQPDGSDAYFDLTAVGKKREEPMFVVPHEDVLTTGPFTLDPQGRNMVMVDSRQ
                     RNTAALFEFNLATHKATLLAQDEKADVQNVLVHPRTQKVQAASSNYERVAWQIVDPDI
                     RPDMEVLKTAYPGDLSVVDRSLDNERWVVAYELDDGPTRYARYDRKDKKVTFLFTSRS
                     SLEGLRLAKMHPVVIRSRDGLELVSYLTLPVDSNPQGGIHPSSPLPLVLFVHGGPWSR
                     DEWGYDSYHQWLANRGYAVLSVNYRGSTGFGKRFVNAGDKQWAAKMHDDLLDAVRWAI
                     DEKVAIPDKVAIMGGSYGGYATLVGLTFTPEVFACGVDIVGPSNLLTLLQTIPPYWEP
                     MIEMFTKRVGDHRTESGRALLRERSPLFRVDKILRPLLIGQGANDPRVKQSESDQIVK
                     AMQKKDIPVTYILYPDEGHGFVRAENRMSFNAIAEIFLAQCLQGPYDPIGNDFNGSSL
                     TVPVGAEAIYGLPGALPTNKTPGK"
     gene            complement(3361..4152)
                     /locus_tag="PK710_09125"
     CDS             complement(3361..4152)
                     /locus_tag="PK710_09125"
                     /inference="COORDINATES: protein motif:HMM:NF018731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1338 domain-containing protein"
                     /protein_id="HOT09920.1"
                     /translation="MTINDLLAAMWRDYIRLNPQADRIARLFADRGEEVVNDHIALRT
                     FNLPGIGIDVLAEPFVRGGYQPRGTYSFPNKKLVAKHFEAPGLPKVFVSELQVELFPP
                     EVREMVTGLVAQMDPSLPERQDFCMVGRPWNLSYDVYTRLREASEYAAWVAAHGFRPN
                     HFTVMVNALRTFQSLQQVNDFLEAQGFVLNDSGGKIKGSAAVLLEQSSVLANTIDVAF
                     DDGIHPIPGCYYEFARRYPGPDGELYQGFVAASADRIFESTDRGQ"
     gene            complement(4176..5801)
                     /locus_tag="PK710_09130"
     CDS             complement(4176..5801)
                     /locus_tag="PK710_09130"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOT09921.1"
                     /translation="MAIRTIYALTPDDPSLDPQGRPAPLSVSTDPDELAPIAKDAGAL
                     EGSGAAGLAVPSSEGQLARWLRDNPAAAVIVQGALTSLTGGATPSDDIVISTKKMTSL
                     RVDPANLRAVVGAGLTLSELQAALADEDLYYPPAPTHDGASLGGNVATNAAGAATFKY
                     GTTRDWVERLRIVLASGDVLELRRGQCVLEPSDRVRIEASVPIEFDVPSYESPALKKS
                     SVGYYGKRPWDLIDLFIGSEGTLGIVTEIEVRLVPRPSVLTGLCFLHSVADTLALVTD
                     LRQRSLAAWAAKTPGMDVRAIEYFDARCLQMLRNSGKLAEHGVDVPPGAVACLLFEQE
                     IDKTLSHDEILERLAAVHEGKEAVEGIGELMAVLMAHEVVEHTELALPSDSRRKRQLA
                     AIREAIPLDISDYLRAQHEKDKEIHKVAGDMIVPMDHFAHMLGRYDEVFRQHGVDVVI
                     FGHISDGNVHPNGLPRQAADMPRAKQALWELAQEAKALGGCPLSEHGVGKHPWKKQML
                     SMYWGQGVIEEMKRIKRALDPKGRLGRGILWDP"
     gene            complement(5963..7282)
                     /locus_tag="PK710_09135"
     CDS             complement(5963..7282)
                     /locus_tag="PK710_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09922.1"
                     /translation="MPYHRVGFGLFLSLCSVVTIVGCSARGVEDPAGTTVPDAESPVD
                     AGADVVEDMADIDEPADEDAFVALDVSSEQGPWDPDSACATTVAEAPTYPLPVDIIWV
                     VDNSASMKPAIDELTQGLNDFANLVASTTIDFRVIMLSLRSKINPVTLPVGKRYGVCI
                     PPPLAGDDDCGNGQRYFQSSIDIRSTQPLEQFLGTLDQTQGYAEGEERGGEPWAQWLR
                     PEATKSIVVVTDDNSRLSASYFETFPGGTNPNNSKYVLPPGILHASRGAVFEDYLFHG
                     LYGWGDDTDPGVKCTYPDSKQPPSSGPTYTELVQKTGGVRAKICDGATAWTPFFESIA
                     QAVIATSKIDCEFEIPPPDDGPINPAAVNVRIVDDQPNGQEQEIPVFKVAGPQACDAS
                     GGWYYDDESDPKRVILCPASCDVAQSVVGVEKNGRIEVAYGCPTEVK"
     gene            7531..9099
                     /locus_tag="PK710_09140"
     CDS             7531..9099
                     /locus_tag="PK710_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09923.1"
                     /translation="MRILGTIMLAGLLWGCGSASASAPKTPTAPQPDDGATTTPLRPS
                     SKGLPVAHGAVPAAPSQCEAYSHAVEEAGACGSDRSETWNRLLEALEAREETRDRRLA
                     ALENCDVFPRGWVRAVRAELAPLACGDVLVEPWLAHVSASEVRRDVQLSLIGLGLGAR
                     LSRLVNEAPRIEPPFDKEGFHRFMKERLRPWIIEQAAAIQELSQEGARLEGYGRAIVA
                     VEAGMADMRFVDVMRSIPLPTEMEADPEIKDIYYGTLDEVLEPRKQRGRDAALVGLRD
                     LAAVGVLRDARVDRARALLSKLYGGRRIDALDGLLLPAMPSWEPETVEQRLAVKLPTF
                     YAGLLLTELNPTDLGNLRAWMERGIPAWGWEKLEQNVWTEEGRRLWARALVLYGQRYW
                     RSGDFARASALVKQDKTPEARLLSALAVALQGGPKDAAEMMVRGPLLPEGVGNVADLD
                     AIARSKSPLAAMASYDAAYLLQLVPPQTPSAPFWRSVAERYEYAAPLLEDPAYRREAS
                     DRAKAARQIAATLR"
     gene            9667..10827
                     /locus_tag="PK710_09145"
     CDS             9667..10827
                     /locus_tag="PK710_09145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HOT09924.1"
                     /translation="MPFSDNVTELTYDHKRLIIVGTAHVSQRSVDEVREVIEQVRPDS
                     VCVELDKTRHDALVDEARWRKLDIFQVIRQKKVLMLMASLALAAFQRRLGDKLGVKPG
                     AEMLAALQKAKEIDAQVVLADRDIQATLKRTWAALSLWNKARVAALLMVALTSKEEIT
                     EERIEELKERDTLNETLQEFAEALPQVKEPLIDERDRFLISSIREAPGETVVAVVGAA
                     HVQGMVSNLEATVDRDALSVIPPAPWSRKLLKWILPLIVIGAFYFGFRQHEGETLERM
                     IYAWVIPNAVLAGILALVAGAKPLTVLLVSLASPITSLNPTIGAGMVGGLVEAWLRKP
                     TVADCERVSEDSTTLRGIYRNAFSRVLLVAVLSTIGSALGAYVGATWVVLLL"
     gene            10892..>11208
                     /locus_tag="PK710_09150"
     CDS             10892..>11208
                     /locus_tag="PK710_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09925.1"
                     /translation="MMAPWDLDKQEASMACERGRWIWVAGMTWLALAGCSFPGYGFPV
                     EGNAGQSGEGGSGGASGANPGGKGGQGGVTGGAGGTGGGTQTGGTGATGGTGGGQTGG
                     TGGG"
BASE COUNT         2324 a         3167 c         3358 g         2359 t
ORIGIN      
        1 cggggctcaa ggaaccatca ggattggcac ttgctttggc ctctttgcac ttggcgggac
       61 cgaccatggt atccgggggc aaggcttcgc cgcgggcaca cttttcgagg tccgccttat
      121 acttttcatt ggtctgagcc atcaattcgg tggcgctcaa cgctttggtg gcgctggcgg
      181 cctgggcgaa catgccatcg agttgcgcct tgtatttggc ttcgtattcc tgatcaccga
      241 aggtttttcg catctgctct tcgtaggttt tttgcgaagc ctccacgttc tgagcgaaat
      301 ggtccacata cgttttcgca tcctccaagg aggcttgccc ttgcaacggg attgcttcga
      361 tccccgcctt cgccacatcg tcaatcaggg aggggtccgc accatggttg acgaccgctt
      421 gataggtggc catcagggcc atactcaggg cagccacagc ctcccgacgg ctctgtcttt
      481 ccatgagttg ccgcaccatt tcataatcct ggtcggtcca ttgcatttcc atctggcgac
      541 gggatcggat catgccggcc acggtggtca tggtagtcat cgagccggtg gtgaccatcc
      601 atccggcatt catcgcggga gtggtagcca ggcggacctg cgctttgagg tcattgattt
      661 tctggacgtg ctgcacgcca tcctgcagga acttgatttc tgcctgggta gcttttttgc
      721 tagccatttt tggcccgatt tcttgcgctc gcttggaata ggagtcctcg agctgcatga
      781 actggttggc aactggcttg ccttgcgcat caaggaccgg gctgatcgat agctcgatct
      841 tggccgcgcg aacctctttg atggcttgcg aagccggcat cggcatggga ccgcccgcaa
      901 aggaagcttc ctgtccgacg gccgccttca tggccttctc gatctccgcc tggttcttat
      961 cgatttcttc gtccatttgc ttctgggccg caaggaccat ctcatccccc tgccgcacca
     1021 tcgccttgga ttcctccgga atggcagagc ccgacggctc ttgcgcaggc tgctggggag
     1081 gaaaacatcc gcccgccaat acggcaacca ccaacgcacc caatcgaata tgacgcatca
     1141 tgagccctcc ttgatcaatc attattttcc aggcgttttg ttcgttggca gtgcccctgg
     1201 caaaccataa atggcctccg ctccaaccgg cactgtcaaa ctcgacccat tgaaatcgtt
     1261 tccgatcggg tcatatgggc cttgcaaaca ctgcgcaaga aaaatctccg caatggcgtt
     1321 gaacgacatt cgattttccg cgcgcacaaa cccatgcccc tcgtccggat ataatatata
     1381 agtcaccgga atatcctttt tttgcatggc cttcacgatt tggtcggatt cggattgctt
     1441 gacccgcgga tcgttggcgc cttgaccaat caacaaagga cgcaaaatct tatccacccg
     1501 aaaaagcggt gaacgctccc gcagcagcgc cctgcccgac tccgtacgat ggtcccccac
     1561 ccgcttggtg aacatctcga tcatcggctc ccaataaggc ggaatggttt gcaatagcgt
     1621 gagcagattc gatgggccga cgatatccac accacaagca aaaacctcgg gtgtaaatgt
     1681 cagtcccacc aaggtggcat atccaccata acttccgccc atgatagcca ctttatcagg
     1741 aattgccacc ttctcgtcga tggcccatcg cacggcatcc agcaggtcat cgtgcatttt
     1801 cgccgcccat tgcttatccc ccgcattcac gaagcgcttg ccaaatcccg tggaaccacg
     1861 atagtttaca ctcaatacgg catacccacg gttcgcgagc cattgatgat acgaatcgta
     1921 tccccactcg tctctcgacc aaggcccccc atggacgaaa agcaccaggg gaagcggcga
     1981 agacggatga atgccccctt gcggattgga atccacggga agcgttagat aactcaccaa
     2041 ttccaaccca tcccgtgacc gaatcacgac cggatgcatc ttcgccaacc gcaatccctc
     2101 caacgaagag cgggaggtaa aaagaaaggt cactttttta tccttgcgat cataacgagc
     2161 ataacgcgtg ggaccatcat ccagctcgta cgccaccacc catcgctcat tatccaacga
     2221 cctatccacc acactcaaat cccccggata agccgttttg agaacctcca tatccggccg
     2281 aatatccgga tcgacgatct gccacgccac tcgctcgtag ttcgacgatg cagcctgcac
     2341 cttctgcgtc cggggatgaa cgagcacgtt ttgcacatcc gctttctcat cctgcgccaa
     2401 caacgtcgcc ttgtgggtag ccaaattgaa ctcgaaaagc gccgcggtat tcctttgccg
     2461 gctgtccacc atcaccatgt tccgcccctg cgggtcgagc gtgaaaggac cggtcgtcag
     2521 tacatcctcg tggggcacga caaacatcgg ctcctcgcgt tttttcccca ccgccgtcag
     2581 atcgaagtac gcgtcgctcc catccggctg ctgtttgatg ccaacccgca accgaaagcc
     2641 atcatcggca agaaatccag aaaacctctc ctcgttttta tatacgagtt gacgcttacc
     2701 cgtcgtcacg tcgatccgat acacatcgtg ccatttggga tccctatcgt tcaatcccac
     2761 caagacctcc cgaggatgct gcgggctggc gtgcagcaga ttggcttgca cgtctttgtc
     2821 gggactcagc acccgttggg cgcccgactc cacatccaac gcgataaggt accagttttc
     2881 atctccgccc ttgtctttcg aaaatacgat atgtttattg tcataggccc actgatagcg
     2941 agtaatgctg cgctcggttt cggaggtgag gggacgggcg tttcgatgat cgagggcagg
     3001 agcaacccaa aggttgagca ccccatcctt gggcgccagg aagctcaacc actttccatc
     3061 gtgactgaga cgcacccgcg cccggtcggg atcgccaaac aataccttgc gcggaatgag
     3121 agacgtatcc tgcctgggaa tggacgtcat gggcgtggaa ggcccggtca ccgccgaagc
     3181 cgaagtcgaa ggcgcagggg aaggcaatgg cgtcaaagcc ggcgaggaac cgccacaggc
     3241 ggccaccagc agacacagaa acggggtggc aaatgtcttc atgcgcgagg tattcgcgcg
     3301 aaaatgttct gaaggcaagc gtcgacccgc gccaaaatca atggacgacc acaaaggctc
     3361 tcattgaccg cggtcggtgg attcgaaaat tctatccgcc gaggccgcga cgaacccttg
     3421 gtacaattcg ccgtccgggc ccgggtagcg acgggcaaac tcataataac acccaggaat
     3481 cggatggatc ccgtcgtcga aagccacgtc gatcgtattc gccaacaccg aggattgctc
     3541 gaggagcacg gcggcggaac ctttgatttt cccgccggag tcgttgagta cgaatccttg
     3601 ggcctccaaa aaatcattga cctgctgcag ggattgaaac gtccgcagcg cattgaccat
     3661 gaccgtgaag tggttggggc ggaacccatg ggccgccacc caggcagcgt attcactggc
     3721 ctcgcgcaat cgcgtataga cgtcgtagct caagttccag ggacgtccca ccatgcaaaa
     3781 gtcctgtcgc tcgggcagcg atgggtccat ctgcgccacg agtcccgtca ccatctcccg
     3841 cacctcgggg ggaaatagct ccacttgcag ctcgcttacg aagactttcg ggagccccgg
     3901 cgcttcgaaa tgcttcgcca ctagcttctt attcggaaac gaataggttc cccgaggctg
     3961 atagccgcca cgcacgaaag gttcggccag cacgtcgata ccgatgccag gcaagttgaa
     4021 ggtgcgaagc gcaatatggt cgttgaccac ctcttccccg cgatccgcaa agagcctggc
     4081 aatgcggtcc gcttgcggat tgagacggat ataatcgcgc cacatggccg cgagcaaatc
     4141 gttgatagtc attgaattca ttaccttttt ctccatcacg gatcccacag gatcccccga
     4201 cccagccgcc ccttggggtc caacgcacgc ttgatgcgct tcatttcctc gatgacccct
     4261 tgcccccaat acatggataa catttgcttc ttccacggat gtttgcccac cccatgctcc
     4321 gacaacggac agcctcccaa cgctttggct tcctgcgcca actcccataa cgcttgtttg
     4381 gcccttggca tatccgctgc ctgccgaggc agcccgttgg gatgcacgtt cccgtcggaa
     4441 atatgcccga aaatcacgac atcgaccccg tgctggcgaa atacctcatc atatcgcccg
     4501 agcatgtggg cgaaatggtc catcgggacg atcatgtcac ctgcaacttt atgaatttct
     4561 ttatcttttt catgttgcgc ccgcagataa tcgctgatgt caagcggaat ggcctcccta
     4621 atcgccgcta gctggcgctt ccttctcgaa tccgaaggca aggccagttc ggtatgttcc
     4681 accacctcat gagccatcaa caccgccatc aactcgccaa tgccctcgac agcttctttg
     4741 ccttcatgga cggcagccag gcgttccagg atctcatcat gggaaagggt tttatcgatt
     4801 tcttgttcga agagcaggca agcgacggcc cccgggggca catcaacccc gtgttctgcc
     4861 aatttgccgc tatttcgcag catttgcagg cagcgggcgt cgaaatattc gatggcccgc
     4921 acgtccatcc ccggggtttt ggcggcccag gcggccagag agcgctgtcg aagatcggtc
     4981 accaacgcca gggtatccgc cacggaatgc aagaaacata gacccgtaag caccgaaggt
     5041 cggggcacga gccgcacttc gatctcggtc acgataccca gggttccctc acttccgatg
     5101 aaaaggtcaa tcaggtccca ggggcgcttc ccataatacc cgaccgagct ttttttcagc
     5161 gctgggctct catagctcgg cacgtcgaac tcgatgggca cgctggcttc gatacgtacg
     5221 cggtccgagg gctcgagcac gcattgtccc cttcgaagtt cgaggacatc gccgcttgcc
     5281 agcacgatac gcagtcgctc gacccaatcg cgggtggtgc cgtacttgaa cgtggccgct
     5341 cccgcggcgt tggtcgccac attgcctccc aagctggcgc catcgtgggt gggagccggt
     5401 ggataataaa ggtcttcgtc ggcaagggca gcctgcaatt cggatagggt gagtcccgct
     5461 cccaccaccg cacgaaggtt ggcgggatcc acccgcagcg atgtcatttt tttcgtggaa
     5521 atgacgatat cgtcactcgg agtggctcct cccgtcaggc tggtcaatgc cccttgcacg
     5581 atcacggcgg cggcgggatt gtcacgaagc cagcgagcga gctggccttc ggaggaagga
     5641 acggcgaggc cagcggcccc cgagccttcg agggcgccgg catcttttgc gatcggggcc
     5701 agttcgtccg gatccgtact caccgaaagc ggcgcgggtc gaccctgcgg atcgagggat
     5761 ggatcgtcgg gggtgagggc gtaaatcgtt cgaattgcca tgataccaag cgggtgatgc
     5821 gtgagcgaat tggatgccgg aaaaaaagat aggttgcggc tatgcccgat gattcatccc
     5881 gccccggaca atgcctgcaa aatcgcccaa ggacacgtcg acgagcgcgc gccttcgggc
     5941 tcgtcgttcc cctcgcaccg acttacttca cctcggtggg acagccatag gcgacttcga
     6001 tccggccatt tttttccacg ccaaccacgc tttgcgcaac atcacaagac gcggggcaga
     6061 gaatgactcg cttcggatcc gattcgtcgt cgtaatacca tccgccgctc gcatcacagg
     6121 cttgcggtcc ggcgactttg aacaccggga tttcttgctc ttgcccgttg ggttggtcat
     6181 cgacgatgcg aacgttgacg gcagccggat tgatggggcc atcgtcgggg ggagggattt
     6241 cgaattcaca atcgattttc gacgttgcga tcaccgcttg cgcgatgctt tcgaaaaagg
     6301 gtgtccaagc ggtggcacca tcgcagatct tcgcgcggac accgccggtt ttttgcacca
     6361 actcggtgta cgtggggccg gagctgggcg gttgtttact atccggatac gtacacttga
     6421 cccccggatc cgtgtcgtcg ccccatccat acagcccgtg aaaaagataa tcttcgaaca
     6481 cggccccgcg actggcatga agaattcctg gtggaagcac gtacttgctg ttgttgggat
     6541 tcgttccgcc cgggaatgtt tcaaaatagc tcgcactcaa ccgggagttg tcatcggtca
     6601 ccaccacgat cgacttggtg gcttcaggcc gcaaccattg tgcccagggc tctccccctc
     6661 gttcttctcc ctcggcataa ccctgggttt gatcgagcgt gcccaaaaac tgttcgagcg
     6721 gttgcgtact gcgaatgtcg atgctcgatt ggaagtagcg ctgtccgttg ccgcaatcat
     6781 cgtcgcccgc caacggaggc ggaatgcata ccccatagcg cttgcccacg ggtaaggtca
     6841 cgggattgat tttgctgcga agagacagca tgatcacacg aaaatcgatg gtggtggagg
     6901 ccacgaggtt cgcgaaatca ttgagcccct gggtcagctc atcgatcgcg ggtttcatgc
     6961 tcgcggagtt gtccacgacc cagatgatat cgaccggaag cggataggtg ggagcttccg
     7021 ccaccgtggt ggcgcaggca gaatcggggt cccagggccc ctgctcgctc gagacgtcga
     7081 gagccacgaa agcatcctcg tccgctggct catcgatatc agccatgtcc tcgaccacat
     7141 ccgcaccggc gtcgacaggc gattcggcat ctgggaccgt cgtaccagcc ggatcctcga
     7201 cccctcgagc actacaacca acgatggtca ccacactgca taagctcaag aaaaggccaa
     7261 agccaacgcg atgatacggc atgaaaggca gtgtagtaca cggctcgtca atcgggtagg
     7321 aatccaaagc cagatgcgat gatgagaagg cgatgatgaa acggaaccgc gggcgcgaag
     7381 cggggccgca aatcatataa ctagataata ttatttaata ttttgttatg cctccacgat
     7441 gctaggcccc cgaaaaacac cgtcatgaaa caaaccgaga tacggagcga gcccatttcc
     7501 caatcatcga agctgtatta gggaggaagc atgcgaatcc ttgggacgat catgcttgcg
     7561 gggctgctgt ggggctgcgg ctcggcttct gcttccgcac cgaaaacacc gacagctccc
     7621 caacccgatg acggagccac cacaacaccc ctgcgaccat cgtcgaaagg gttaccggta
     7681 gctcatggtg cggttcccgc ggcaccatcg cagtgtgagg cgtattccca tgcggtggag
     7741 gaggcggggg cttgtggttc ggatcgttcg gaaacgtgga atcggttgct cgaggcgttg
     7801 gaggccaggg aggagacgcg ggatcgtcgg ctggccgcac tcgagaattg tgacgtattt
     7861 ccgaggggtt gggttcgagc cgtgcgcgcg gagctggccc ctttggcatg cggcgacgtg
     7921 ttggtggagc catggctggc gcatgtttcg gcttcggaag tccggcgaga cgtgcagttg
     7981 tcgctgattg ggttggggtt gggagcgcgg ctgtcacgcc tggtgaacga agctccccgc
     8041 atcgagccgc cgttcgacaa agagggtttc catcgattca tgaaagagcg tctgaggccc
     8101 tggattatcg aacaagcggc agcgatccaa gagctatcgc aagagggggc gcgtctcgaa
     8161 ggctatggtc gcgcgattgt ggcggtggaa gcgggcatgg cggatatgcg gttcgtggac
     8221 gtgatgcgga gcattccgct tccgacagaa atggaagcgg atcccgagat caaagatatc
     8281 tattacggca ccctggacga ggtgctggag cctcgaaaac agcgaggtcg ggatgcggcg
     8341 ttggtggggt tgcgcgattt ggccgccgtg ggggtgttgc gcgacgcgcg ggtggatcgg
     8401 gcgcgtgcgt tgttgtcgaa gttgtatggg gggcggcgga tcgacgcgct ggatggtttg
     8461 cttttgcccg cgatgccgtc gtgggaaccg gaaacggtgg agcagcgctt ggcggtgaag
     8521 ttgccgacgt tttatgcggg attgttgttg acggagctga atccaacgga tttgggcaat
     8581 cttcgcgcgt ggatggagcg gggcatccca gcgtgggggt gggaaaagct cgagcagaac
     8641 gtgtggacgg aggaggggcg acggctttgg gcgcgggcct tggtgcttta tggacagcgg
     8701 tattggcgaa gcggagattt tgcgcgagcg tcggcgctgg tcaaacagga caagacgccg
     8761 gaggctcggc tgctttccgc gttggcggtg gcgctgcagg gaggaccgaa ggatgcggcg
     8821 gaaatgatgg tcagagggcc attgttgccg gaaggggtgg gcaacgtggc ggacctggat
     8881 gcgatcgcgc gttccaaaag ccctctcgcg gccatggcat cgtatgatgc ggcctacctg
     8941 cttcagctcg tgcctcccca gaccccgagc gcgccatttt ggcggtccgt ggcagaacgt
     9001 tatgaatatg ccgccccttt gctcgaagat cccgcgtatc gccgtgaggc tagcgaccgg
     9061 gccaaagccg cacgtcagat agccgctacg cttcgttgaa gcgggggata gggaaaaccc
     9121 acgctggcaa aagggcacag gcgcttcaac aagagcaaca tccggtatac catacgggcg
     9181 ggtttgaaac ccgcccctac aggcgacatt ggcgagaggt tgaaatcccc tggaaacaac
     9241 ggggggatgg cttagcacgc ctagacgccc agatttccgc tccctctttc cctcttgtta
     9301 aagcgcctgt gggcgaaaag cggatcgctc catatataaa ccaataaatt caatatatta
     9361 tatcggattc acggatagaa ttgaccggac aaaaaaagtc gcgcgagggg ggggctacct
     9421 cgcgcgacac acgaaacagc gtgacgattg gaggggaggg atgcggaccg tcacgccgag
     9481 ttttcgttga gcaggcggcg tgccacccgg aaacgacgct ttgtcgcaac gttctcctcg
     9541 gattcatcag ccagcgtttc gagactgcaa tggcgcatct cgtgattgcg acatcctcat
     9601 gggataagtg cattccgttt tcgttgcctc ttgcatgaac ggccctttcg gtccaggtac
     9661 tcatccatgc cgttttccga taacgtcacc gagctgacct acgaccacaa aaggttgatt
     9721 atcgttggca cggctcacgt atcccagcgt agcgtcgatg aggtacgcga ggtcatcgag
     9781 caagtccgtc cggattccgt ttgtgtcgag ttggacaaga cgcgccatga tgcgttggtc
     9841 gacgaggcaa ggtggcgcaa gctcgatatt tttcaggtta tccggcaaaa aaaggtattg
     9901 atgctgatgg caagccttgc gttggccgcg tttcagcgtc gattggggga taagctcggg
     9961 gtaaagccgg gcgcggagat gctggcggcc cttcagaagg ccaaggagat cgacgctcag
    10021 gtggtgcttg cggatcggga tattcaggca acgctcaagc gaacctgggc ggccttgtcg
    10081 ttatggaaca aggcgagagt ggcggcgttg ttgatggtgg ccctgacgtc gaaggaagag
    10141 attacggaag agcgaatcga ggaactcaaa gagagggata cgctcaatga aacgctgcag
    10201 gagtttgcgg aagcgcttcc ccaggtgaag gagccgctga tcgacgagcg ggatcgattt
    10261 ttgatatcgt cgatacggga ggcgccagga gaaacggtgg tggcggtggt gggagcggcg
    10321 cacgtacaag ggatggtgtc gaatttggag gcgacggtcg atcgcgacgc gttatcggtg
    10381 attccgccgg cgccttggtc acgaaaactg ctcaagtgga tattgccgtt gatcgtcatc
    10441 ggagcgtttt attttggttt tcggcagcat gaaggggaaa ccttggagcg gatgatttac
    10501 gcgtgggtga taccgaatgc ggtgctggcg gggattcttg cgctggtagc gggggcaaag
    10561 ccgttgacgg tgctattggt gtctctggcg tcaccgataa cgtcgttgaa tccgacgatt
    10621 ggggcgggaa tggtgggggg attggtggaa gcgtggctgc gcaagccgac agtcgcggat
    10681 tgtgagcgtg tttcggagga cagcacgacg ctgcggggga tttatcgaaa cgctttctcg
    10741 cgggtattgt tggtggcggt attgtccacg attggttcgg cgcttggggc ttatgtgggg
    10801 gcaacctggg tggtgttatt gctttgaaag ggggctgctg gctttttgcg ccttcccctg
    10861 gggcgaaggc ttgctatgct gaggcgagac gatgatggct ccgtgggatc tggacaagca
    10921 agaggcttcg atggcgtgtg agcgagggcg ttggatttgg gtagccggca tgacgtggct
    10981 ggccttggcg ggatgttcct ttccgggcta cggatttccc gtcgaaggaa acgctgggca
    11041 gtcgggcgaa ggaggctcgg ggggagcgtc gggggcgaat ccaggaggca agggtggaca
    11101 gggcggggtt accggaggag cgggcggcac ggggggagga acgcagacag gaggcacggg
    11161 cgcgacaggg ggaacggggg gcggacaaac gggcggtaca ggcggggg
//
LOCUS       DAOSOR010000130        11163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5816_length_11163_cov_4.732625, whole genome shotgun sequence.
ACCESSION   DAOSOR010000130 DAOSOR010000000
VERSION     DAOSOR010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11163
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5816_length_11163_cov_4.732625"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /locus_tag="PK710_09155"
     CDS             complement(<1..1467)
                     /locus_tag="PK710_09155"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOT09926.1"
                     /translation="MSRFRQIFAIVVALSTATLPGPKNAKACTSFLVSKGASVDGSTM
                     ITYAADSHDLYGELTFTPAAKHKNGAKRTIRDWDSNKVLGQIDEVPETYRVLGNMNEH
                     QLVIGETTFGGRKELEDPKGKIDYGSLIMIALERAKTAREAIRVMTDLANEHGYASSG
                     ESFSIADPNEVWIMDLIGKGPDQKGVVWVACKVPEGYVTAHANQPRIRTFPQNDPEQC
                     VYAKDVVSFARSKGYYEGPDDRFSFVDAYATQTCRDLRVREARVWAFYNQVAPSLKLS
                     PDYAACKPGAEPYPLWIKPDRKLSVRDLILGMRNHFENTVFDMRKDVGAGPHGLPYRW
                     RPLTWKHEGKTYVHERATATQQTGFSFVSQSRAWLPHEIGGVLWFSVDDAASTVYVPM
                     YAGIREVPKPYAVGTGDFQRFSWDSAFWVFNGVSNFTYLRYEDMIKDVEKAQQQLENG
                     FFDEQATFEKAVLDKHGKSKDSAVKELTRYSREASDKVL"
     gene            complement(1566..3056)
                     /locus_tag="PK710_09160"
     CDS             complement(1566..3056)
                     /locus_tag="PK710_09160"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOT09927.1"
                     /translation="MTEESQSTMESATETDLSPQDLPLDPPEASNDAVKQTVADGEDD
                     DGPADRREGSPRPELLQLVDLAAKYPEIGPPLAQLAFKIGQPEFGNQIVRMGLDKEGP
                     GLEYYFVVAHSARRQRRYAEARKLSVDAIEAFLRTADDELASDDGERLLHLIRLGFST
                     LLFDEKDPKGDLPFIERLREALPRLAPRLSSEAFFHALLAQTLWYDDIEASEQAWDRA
                     AELDTTEATWNARGTWYKDAQGDLDKAEQAYRQGLQIVPTSPLLLHNLGQLLVDKAER
                     PDVDVESARRLLHEAEPILRAALREESPKGLRRHVHSTRDRLMAFRASLPPRAPRKEE
                     PEPQREPVVGEVLKGKVRSLTAFGAFVVLPECGTGLLHKSELAHEPIDDPAQLLQVGE
                     EVEVKVLEVERRDGKLRIALSRKALLPRPEAAPPPATQRPRHEGRPDGQQGHHPRRED
                     PRDRKGGPRRGPDDRTRPRQGKPKDDDKFASLGEMLLAKMNQQKKT"
     gene            complement(3148..4002)
                     /locus_tag="PK710_09165"
     CDS             complement(3148..4002)
                     /locus_tag="PK710_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09928.1"
                     /translation="MRLSWEARMNMSQLRRFIALGLLAAIGMTMGCDEDAKCKEAEAC
                     KKQGKCKVDVKDGCIAGKAEDCKASVECKTLGKCSLKERVCVAASEAECKAAERCKTD
                     GLCDLHEDGCVDLGKLFFPDCSVECKSDGHCVKREGKCLALSNHHCMGTVDDKPEADS
                     VCRTEGRCTVRDGDCKALTDKECESSEACTKDARCLAKDGKCVATEKGCAESDICRRA
                     GRCTLKDGQCVVASSADCKKSARCELEGLCTLKDGKCIAATSADCARAGVCTKAKRCR
                     AEDGACTK"
     gene            complement(4161..5369)
                     /locus_tag="PK710_09170"
     CDS             complement(4161..5369)
                     /locus_tag="PK710_09170"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HOT09929.1"
                     /translation="MKSSTRLGSGHCFRRQVALWGIVGMVGLCTACGDSASESDGSDQ
                     GRVIPEESSPSEANDPVSSLEPQEKAGEESKGQENSQAPGNNAGEAGEEEEGEPGDID
                     EPQEKGQEELGELFAGVALECQKEIVSGLNTDWQVEGRKRAFYVDFPNNATTAPALVF
                     SFHGFGDNPSAWRQYLGLDPNIDPEFPMIVVTPESTHLQPLNVGNEPQGLDWDLANGG
                     KQYKNRDVSLVQSIAACLRVQNDVDTNRIYSLGFSAGSVFTSLLHSRFPHDFAAIVVM
                     SGMWFNDPAERALINPMVPLNFAWDSLESSASANVLLSRGGPSDAISILEFPVVNLED
                     AAQAAFPFLAAAGRMVVDCPHQNGHRPSTLSRSQLVQFFKDHERAKPSPYLTHGLDST
                     LSAKCALRAP"
     gene            complement(5737..7119)
                     /locus_tag="PK710_09175"
     CDS             complement(5737..7119)
                     /locus_tag="PK710_09175"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HOT09930.1"
                     /translation="MAAPGCAQISGVDDLAFDRKRDAGMASDAPADADDHDEFDTQEQ
                     FDGPTEEDGPSPDVDVPDGPSGTPYFGELKLRCKLINGKSRQDPTANNTHYRANLSGS
                     SLGISVVQDNQLFLFFGDTLGAEKIWQSSTSLPDAVGYAAVDFASVADDPDLLCTELR
                     FLLSGDKTGNVEGDFAGARMKAPAGSSLKEFIHNPAGPLNYAMQPDMPGTLEVPSGAF
                     SHDGSIYLFYTIVSTITLQVGGSYLAKWETPSTTGDPTYQILYHVDQRFTSNGPLGGN
                     FINIAPLVEGEYVYLFGTGKYRNSPVYLARKRLSELAEEGGFEAYDPAMSSWVEAGTP
                     TAPVVAMPSIGELSVQYFPEIQRYVMIDQEVNAGNRIVARFAEKPQGPFSNAVTMAVM
                     EDPAFEKKYCCDAKECVGDKIIQCDQAGFFGVYLLPTVTVHPNASFTLYFTMSTWQPY
                     NVVLMSATFK"
     gene            complement(7252..8223)
                     /locus_tag="PK710_09180"
     CDS             complement(7252..8223)
                     /locus_tag="PK710_09180"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOT09931.1"
                     /translation="MSKRNLSAAGLALTLGLWPMPLLGAPSEAEKETARHLMEQGDEH
                     YEAGRYEQALASYQQADALMGVPTTGLAVAHALDKLGRLRQARKIALRVALSQPGEGE
                     PSVLTEARNESASLAHELAKAIPTLQIVVTGPTSVTLVVDGGAPIAGLSEPISLDPGP
                     HEYRIMAEGYRTIQRRVTLERGERRKVEHQLGQVVQPPPPKAQKNSSIPLVTYIGLGV
                     GAAGLVAGSTTGILSLSKASKAKQYCEGVHCYPEAEAEAERARSLATASNLSFGIGLV
                     GIGVGVMTWLVSPSNGGEASAKKAQQEYWFEVSTGAGASEIQLTHRF"
     gene            complement(8246..9751)
                     /locus_tag="PK710_09185"
     CDS             complement(8246..9751)
                     /locus_tag="PK710_09185"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT09932.1"
                     /translation="MSGSRSSPSLPVTIGRYELRAELAHGGMATVFLGRLEGVAGFSR
                     AVAIKRLHPQFVKDPSFVAMFADEARLTACIHSPNVVSINDVVMYQGELLLVMDFVHG
                     ASLSVLFEVMRQRKQTIDMGIVARIIYDTLNGLHAAHEAQDSSGKPLSIVHRDVSPQN
                     ILVDVAGVARVIDFGVAKAASRFQNTLEGQLKGKLHFMAPEQIRNQRVDRRADVFAAA
                     VVLWTTLAGRRPFESENEANIVFSILEGRRPDLHTFAPSVPPALKAVIDKGLSLDPNE
                     RYCTAANMAEALESAIKLASQREVARWVHETVPSDIEHQAALRFDVESSVNSGHLSSV
                     HHTADVMDEEPPRSAAPSNADLESIHADRLSWSNPSRISSISIAKEPSFATGRPTHQH
                     SIRTAAAIVGPVVMLVIVAGWWLTARSSPPAEASIAAFSTISSTRLSLAMASTSVVSP
                     SPEPMETIAPAVSSSATLPLGSTSTNARPRVKPARTVPAPSTKKKRKDYGF"
     gene            complement(9814..>11163)
                     /locus_tag="PK710_09190"
     CDS             complement(9814..>11163)
                     /locus_tag="PK710_09190"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HOT09933.1"
                     /translation="GKGGSSSGKGGSSSGKGGSGGTGGSGGTGGSGSATGGSGGDDPE
                     ETGGSGGGNPYSEAIKLKRLEVLCKLISDRNIDDPTPNNTHYRANLRGTDLGISVAHG
                     NDLYIFFGDTAGAQHIWPLGPESLPDAVGYSAVPYSSVAQNPSLLCSNLRFLLTGGKT
                     GNVEGDFAGASMQPPAGEPISNYIHNPAGPRGANMFPHLPGDFEVPSGAFSYGGSIYV
                     FYTTVNLNPFEMRGSYLAKWEHPSTGGKPDYRILYQVDQRFDSQGPLRGKFINIAALV
                     DEPYLYLYGTGKYRASSVHLARKRLSDLDTPGGFEVFDATSQQWVNPKSSAGPVVSLP
                     SIGELSVQHYPSIGRYVMMDQELTNIVARFADKPEGPWGNAVTVASMIDPIFALTYCC
                     INLDCPGKRLINCDRAGFYGTYMLPDPIVHADGSFSIAFTMSTWDPYNVALMSATFQ"
BASE COUNT         2410 a         3433 c         2912 g         2408 t
ORIGIN      
        1 aaggaccttg tccgacgcct ctcggctata gcgggtcagc tccttgaccg cggaatcctt
       61 ggactttccg tgcttgtcca gcaccgcttt ttcaaacgtc gcctgctcgt caaaaaaacc
      121 gttctcgagc tgttgctgcg ctttttcgac atccttgatc atgtcttcgt agcgtaagta
      181 ggtgaagttc gacaccccat tgaaaaccca gaaggccgag tcccacgaaa agcgctgaaa
      241 atctcctgtt cctacagcgt aaggcttcgg tacttcccgg atccccgcgt acatcggcac
      301 atataccgtg ctcgccgcat catcgacgga aaaccacaag actccaccaa tctcgtgggg
      361 aagccatgcc cgagattgac tgacaaacga aaaccccgtc tgctgtgtgg ccgtggcccg
      421 ttcatgaaca taggttttcc cctcgtgctt ccacgtcaga ggacgccatc gatagggcaa
      481 accgtgcggt cccgccccca cgtccttgcg catgtcgaaa acagtatttt cgaagtgatt
      541 tcgcatacct aggataagat cgcgcaccga cagcttccga tccggcttga tccaaagagg
      601 ataaggttcc gcccccggct tgcacgccgc ataatcggga gaaagcttca aagaaggcgc
      661 cacctgattg taaaacgccc aaacccgcgc ctcccgaacc cgcaaatccc gacaggtttg
      721 tgtcgcatac gcatccacga acgaaaaacg atcgtcgggc ccctcataat atcctttgct
      781 cctcgcaaat gacaccacat ccttcgcata aacacattgc tccgggtcgt tctgcgggaa
      841 agtccgaatc cgaggctgat tggcatgcgc cgtcacatac ccttccggaa ccttgcacgc
      901 gacccatacc acaccctttt gatccggccc tttgccaatc aaatccataa tccacacttc
      961 attcggatca gcgatggaaa acgattcccc cgaactcgca tacccatgct cgttggcaag
     1021 atccgtcatg acccgaatgg cctcgcgagc cgttttcgcc cgctccaacg ctatcatgat
     1081 caggctccca taatcaattt ttcccttggg atcctcgagt tccttgcgcc caccaaacgt
     1141 cgtctcccca atgacgagtt gatgctcgtt catatttccc agcacacgat aggtctcagg
     1201 tacttcgtcg atctgcccca ataccttgtt ggaatcccaa tcccggatcg tgcgcttggc
     1261 cccgttttta tgtttggccg cgggcgtaaa cgtcaactcc ccataaaggt catgggaatc
     1321 ggcggcgtag gtgatcatgg tcgagccatc caccgacgct cctttgctca ccaaaaaact
     1381 cgtacaggcc tttgcgtttt tcggccccgg aagggtcgct gtcgacaagg caactacgat
     1441 ggcaaatatc tggcgaaatc gagacatcgg catctccaag gcgataatga ccccgccgcc
     1501 aaaccatcgc ctggccgcgg cgacaaccca cgatcgtttg catttgcgct tccctaagga
     1561 agccatcatg tctttttttg ctggttcatc tttgccagca gcatttcccc aaggctcgcg
     1621 aacttatcat cgtctttcgg cttgccctgt cgaggccgcg tccgatcatc cggcccgcga
     1681 cgcggccccc cttttcgatc gcgcggatct tcccgccgag gatgatggcc ctgttgccca
     1741 tcgggcctgc cctcgtgccg aggtcgctgc gttgccggcg gcggcgctgc ttctggtctg
     1801 ggaagaagcg ctttgcgcga gagggcaatc cgaagcttgc catcccgacg ctccacctcc
     1861 aataccttga cttccacttc ttcgccgact tgcagcagtt gcgctggatc atcgatgggt
     1921 tcgtgcgcca attcggattt atgaagcaac cccgtaccgc attccggcaa gaccacgaag
     1981 gcgccgaaag ccgtaaggga tcgcactttg cccttcaaca cttctcccac gaccggttcc
     2041 cgctgtggct ccggctcttc ttttcgcggc gcccgaggcg gcaacgaggc tcgaaaagcc
     2101 atgagccggt cccgggtgga atgcacgtgg cgacgcaatc ctttgggaga ttcttcccga
     2161 agagcagccc gaaggatggg ctcggcttca tgcagcaaac gtcgcgcgct ttccacatcc
     2221 acatcgggcc gctcggcctt gtccaccaat aactgcccga gattgtgcaa caacaagggg
     2281 ctcgtgggta cgatttgaag cccctgacga tacgcttgct cggctttgtc caggtcccct
     2341 tgcgcatcct tgtaccacgt accacgcgca ttccaggtgg cttcggtggt gtccaactcc
     2401 gccgcccgat cccaggcttg ttcactggcc tcgatgtcgt cgtaccaaag ggtttgcgca
     2461 agaagcgcat gaaaaaacgc ttcggaagaa agcctcggcg caagccgggg aagcgcctcc
     2521 cgcaaccgct cgatgaacgg aagatcccct ttcggatcct tttcgtcgaa taataacgtg
     2581 gaaaatccaa gccttatcaa gtgaaggagc cgctccccat cgtcggaagc cagttcgtcg
     2641 tcggccgtac gcaaaaacgc ttcgatggca tccaccgaaa gcttgcgagc ctcggcatag
     2701 cgacgctgcc gacgcgccga atgagccacg acgaagtaat actcgagtcc aggaccctcc
     2761 ttgtccaacc ccatgcgcac gatctggttg ccaaactcag gttgcccgat tttgaaagca
     2821 agctgcgcca ggggaggacc gatctccgga tattttgctg caagatcaac aagttgcagc
     2881 agttccgggc gagggctgcc ctctcgacga tccgccgggc cgtcgtcgtc ttccccgtca
     2941 gctacggttt gctttaccgc atcgttcgaa gcctcgggcg ggtcaagggg taggtcctgt
     3001 ggcgatagat ccgtttccgt cgcagattcc atggtgcttt gagactcttc ggtcacgtcg
     3061 attccaatcc ttccttgccc cgcggcgccg acacaagctt gcgtggccag ccaatgcaag
     3121 cctggacgat ggctttcggc aggcagacta tttcgtacaa gctccgtctt ccgcccggca
     3181 tcgcttggcc ttggtgcata cgcccgcccg cgcgcaatcc gcactggtgg ccgcaatgca
     3241 ctttccgtct ttcagcgtgc aaagcccttc caattcacaa cgcgcggact ttttgcaatc
     3301 cgcgcttgat gccaccacac attggccatc tttgagcgtg caacggccgg cgcgacgaca
     3361 aatatccgat tccgcacagc ccttttccgt cgctacacac ttgccatcct tcgccaaaca
     3421 gcgcgcatcc ttcgtgcagg cctccgagct ttcacattcc ttgtccgtca aggccttgca
     3481 gtcgccgtcg cgcaccgtgc atcgtccctc cgttcgacac acactatccg cctccggctt
     3541 gtcatctacc gttcccatgc agtgatggtt ggataacgcc aggcactttc cctcgcgctt
     3601 gacacaatga ccgtcggatt tgcattcgac gctacaatcg gggaaaaaaa gctttccgag
     3661 gtccacgcag ccgtcttcgt gtagatcgca aaggccatcg gtcttgcatc gctcggctgc
     3721 tttgcactcg gcttcgctcg ccgccacgca aaccctttct ttgagcgagc actttcccag
     3781 cgtcttgcac tccaccgagg ccttgcaatc ctcggctttg ccggcgatgc acccgtcttt
     3841 cacatctacc ttgcatttgc cttgcttttt gcaggcctca gcctccttgc acttggcatc
     3901 ctcatcacag cccatggtca tcccgatggc tgcaagcagc cccagggcaa tgaatcgtcg
     3961 aagttggctc atgttcattc gcgcctccca agacagtcgc atgctgaaac ggctggtgcc
     4021 ataccatcat gctccgcgag ccgcaatcga cggaagcttt ggggtgatga cccagggtgt
     4081 ccttttgccg ctcgcgacgg aggccggggg aaatatggta actatatgta aatattgatt
     4141 aatttacaat gaggcatcga tcaaggcgcg cgcaaggcgc atttggcgct gagagtcgag
     4201 tcgagcccat gggtcaggta aggcgacggt tttgctcgtt cgtggtcttt gaaaaactgc
     4261 accaattggc ttcgggacag ggtggagggt cgatggccgt tctgatgcgg gcagtccacc
     4321 accatgcgcc ccgccgcggc aaggaacgga aacgccgctt gcgctgcatc ttcgaggttc
     4381 acaaccggaa attcaaggat gctgatggca tccgaagggc caccccgaga gagcaaaaca
     4441 ttggcggaag ccgacgattc caaagaatcc caggcgaagt tcagcgggac catagggttg
     4501 atcaacgccc gctcggcggg gtcgttgaac cacattccgg acatgaccac aatggcggca
     4561 aagtcgtgtg gaaagcggga atgcaaaagg gaggtaaata cggatcccgc actgaatccc
     4621 aaagagtaaa tccgattggt atccacgtcg ttttgcaccc gaaggcaggc ggcaatgctc
     4681 tgcaccagcg agacatcgcg atttttgtat tgttttccgc cattggcgag atcccaatcc
     4741 agcccctggg gctcattccc aacattgagc ggttgcaaat gcgtcgactc cggtgtcacc
     4801 acaatcatcg ggaactcagg gtctatattg gggtcgagcc ccagatattg ccgccaggcg
     4861 ctcgggttat ctccaaaacc gtggaaagag aataccaagg cgggagctgt cgtggcgttg
     4921 ttcgggaaat caacgtaaaa cgcccgtttt ctaccttcga cctgccaatc ggtgttgagc
     4981 ccggatacga tctccttttg gcactccaac gccaccccag cgaacaactc ccccagctcc
     5041 tcctgcccct tttcctgcgg ctcatcgata tccccgggtt cgccttcttc ctcctctcct
     5101 gcctctcccg cattgttccc cggcgcttgg gaattctcct gccctttcga ttcctcccca
     5161 gccttttcct gcggctccag cgaggaaacc ggatcgtttg cttcggatgg agacgattcc
     5221 tccggtatca ccctcccttg gtcagaacca tccgattccg aggcggaatc gccacaggcc
     5281 gtacaaagcc cgaccatccc cacgatcccc cacagcgcta cctgccgtcg gaagcagtgc
     5341 cccgagccga gtcttgtcga tgatttcatc cgtcacctcg atctattcgg ttgtccttcg
     5401 tcatcgtcat catagggcac gaatcatgcc gaggtcggac agcggtcttt ccttctgcgc
     5461 ggaaaggact gccgtcatag gcgtaaacac cctcgacaat ggtatgatcg ctaacgagcc
     5521 ccagcggaac gcggtatctc cttacccctt cagcccgtag ccgtagcccc gggcttcttc
     5581 gctccccgga actatcatgg ccgcggcgga tccatccgat ccgctcgaga gtagtacgcg
     5641 gccctcgatg atgcgaccgt gaagaggggc gttgttcccg ccattctagc gcaactaatt
     5701 gaaattgttg ttataattct gaacagggga gcggggttat ttgaatgtcg cactcatcaa
     5761 taccacattg tacggctgcc aggtcgacat cgtgaagtac aaggtgaaag acgcattcgg
     5821 gtggactgtc acggtgggaa gcaaatagac gccaaaaaaa ccggcttgat cgcattggat
     5881 gatcttgtct cccacgcact ctttggcatc acagcagtat tttttctcga aagcaggatc
     5941 ctccatgacc gccatggtca ccgcattgct gaaagggcct tgcggttttt cagcgaaccg
     6001 ggccacgatt cggttgccgg cattgacctc ctggtcgatc atcacgtaac gctgaatctc
     6061 cgggaagtat tgaacggaga gttcgccgat gctcggcatg gcaaccaccg gagccgtggg
     6121 agtcccggct tccacccacg atgacatcgc cggatcgtag gcttcgaatc cgccttcctc
     6181 cgccagctcc gacagtcgtt ttcgcgccaa atatacggga ctatttcgat atttacccgt
     6241 tccaaacagg tatacgtatt caccttccac cagcggtgcg atattgatga agttgccgcc
     6301 cagggggccg ttgctcgtga atcgctgatc cacatgatac aaaatctgat aagtaggatc
     6361 cccggtcgtc gatggcgttt cccatttggc gagatacgat cctcccactt gtagggtaat
     6421 cgtgctgacg atcgtataaa ataggtagat ggagccatcg tgggaaaaag cccccgaagg
     6481 aacttccagt gttcccggca tgtccggctg catggcgtag ttgagcggac ccgcgggatt
     6541 atgaatgaat tctttgagcg aggagccggc aggtgccttc attctggctc ccgcgaagtc
     6601 tccttcgaca tttcctgttt tatcgccgct aagaaggaat cgaagctcgg tgcaaagaag
     6661 gtccggatcg tccgctacgc tcgcaaaatc aacagccgca tagccaactg catccggcag
     6721 cgatgtagag gactgccaga ttttctccgc ccccagcgta tctccaaaaa acagaaaaag
     6781 ttggttgtcc tggaccaccg aaataccaag gctcgaacca ctgagattcg cacgataatg
     6841 cgtgttgtta gcggtgggat cctgccggct cttgccgttg atgagtttgc aacgtaattt
     6901 caactccccg aaatagggcg taccggatgg cccatccgga acatcgacgt caggcgatgg
     6961 cccgtcttcc tccgttggcc catcgaattg ctcttgcgtg tcgaattcat catggtcgtc
     7021 ggcatccgct ggtgcatcgc ttgccattcc ggcatcccgc ttgcgatcaa aagcgagatc
     7081 gtccacccct gagatttgcg cacacccggg cgccgccatg acaatagcga aacagccgct
     7141 tatgaaccac cgaacttcag acaaggtcga tatcgaaaag gtagcagcct tttcgcccca
     7201 aggaaaccac gaaagatgtg ggaaaacgag gtgctcgacg cgaacaactg gctagaacct
     7261 gtgcgtgagc tgaatctccg aggcgccagc tcccgtggaa acttcgaacc aatactcctg
     7321 ctgcgccttc ttggccgatg cctcaccccc gttcgaagga gaaaccaacc acgtcatcac
     7381 gccaacccca atgcccacta gcccgatccc aaacgaaagg ttcgacgcgg tagccaaact
     7441 cctcgcacgc tccgcctccg cctccgcctc cggataacaa tgcacccctt cgcaatactg
     7501 ctttgccttc gaggctttcg acaacgacag aatacccgtg gtgctgccgg ccaccaatcc
     7561 cgccgcgccc acaccgagcc cgatatacgt aactaatgga atagacgagt ttttttgtgc
     7621 ttttggaggg ggtggctgca cgacttgccc aagctgatgc tccaccttgc gtcgctcacc
     7681 gcgctccaac gtcactctcc tttgtatcgt cctataccct tccgccatga tgcggtattc
     7741 atgcggtcca gggtccagcg aaatgggttc ggaaagcccg gctatcggcg caccaccatc
     7801 cacgaccaat gtcacgctcg tcggtcccgt caccacaatc tgtagcgttg ggatcgcttt
     7861 ggccaactca tgcgccaaag aagcgctttc gtttctcgct tcggtgagga ccgaaggctc
     7921 tccttcccca ggttggctga gggccacgcg caaggcaatt ttccgggcct gccgtaatcg
     7981 acccaacttg tcgagcgcat gagcaaccgc caagccagtt gtaggaaccc ccatgagcgc
     8041 atcggcttgt tggtacgagg cgagcgcttg ctcataacgg cccgcctcat agtgctcatc
     8101 cccttgttcc atcagatgac gggccgtctc tttttcggct tccgaaggag cccccagcaa
     8161 tggcatcggc cacaacccca gggtcagagc caatccggcc gcggaaaggt tccgtttgct
     8221 cacgccaccc caccgaagga aaagcctaaa atccatagtc ctttctcttt tttttcgtgg
     8281 aaggggcagg cacggtacgg gctggtttca cacgcggtcg tgcgtttgtc gatgtcgagc
     8341 caaggggcag ggtggcgctc gaagataccg caggcgcaat cgtttccatc ggttcaggag
     8401 aaggcgagac tacggaagtc gacgccatag ccaacgacag cctggtgctc gaaatcgtag
     8461 aaaaagcggc aatagacgct tccgccggtg gactcgagcg agccgtgagc caccatcccg
     8521 ccacgatgac caacatcacg actggtccaa cgatggcggc tgctgtccgt atcgagtgct
     8581 gatgggtggg tcgccctgta gcgaaggaag gctctttggc aatcgagatc gagctgatcc
     8641 gggaaggatt ggaccaggac aacctgtcgg catggatcga ctcgagatcc gcgttcgaag
     8701 gtgccgcgga gcgtggaggc tcctcgtcca tgacgtccgc tgtgtggtga acggaagata
     8761 aatgcccgga attgacgctg ctttccacat cgaaacgcaa tgccgcttga tgctcgatat
     8821 ccgaggggac cgtctcgtgg acccagcggg ctacttcccg ctgagaagcg agtttgatgg
     8881 ctgattccag agcttcggcc atgtttgccg cggtgcaata ccgttcgttc ggatccagac
     8941 tcaggccttt atcaatgacc gctttcaggg cgggtggtac gcttggtgcg aaggtatgaa
     9001 ggtcggggcg gcgcccctcc agaatggaaa atacaatatt ggcttcgttt tctgactcga
     9061 aaggacggcg tccggccagg gtagtccaca agacgacggc tgcggcgaat acatccgctc
     9121 ttcgatcgac ccgttggttt cgtatctgct cgggtgccat gaaatgaagt ttgcctttga
     9181 gctgaccctc gagcgtattt tggaaacgac tcgctgcttt cgcgacgcca aaatcaatga
     9241 cccgcgccac gcccgccacg tccaccaaga tattttgcgg tgaaacatcg cgatgtacga
     9301 tggataacgg ttttccagag ctatcttggg cttcatgggc cgcgtgaaga ccattgagcg
     9361 tatcgtagat aattcgggcc acaatcccca tgtcgatcgt ctgttttcgc tgacgcatca
     9421 cctcgaacag tacgctgagc gatgctccgt gaacgaagtc catgaccaac aacaactcgc
     9481 cttgatacat cacaacgtcg ttgatggaaa cgacgttggg gctgtggatg caggcggtca
     9541 gccgcgcttc atccgcgaac atggcgacaa aagaaggatc cttgacgaat tgaggatgga
     9601 ggcgtttgat ggcgacggct ctcgaaaacc cagccacacc ctcgagcctc cccaaaaaca
     9661 ccgtggccat tccgccatgc gcaagctccg cacgcagttc atagcgaccg atggtcacgg
     9721 gcaatgacgg ggacgagcga gagccggaca cggtattagc ttaccacgct ggaatgaaag
     9781 cgaaaagtag gcattccatg ggacggcgct ggcttattgg aaggtggcgg acatcaaagc
     9841 gacgttgtac gggtcccagg tcgacatggt gaatgcgatc gaaaaggaac catccgcatg
     9901 gacgatcgga tcggggagca tgtaggtacc gtaaaaccca gcgcgatcgc agttgataag
     9961 acgttttcca gggcaatcga gattgatgca gcaataggtc agcgcaaaaa tgggatcgat
    10021 catcgaggca accgtgaccg cattgcccca aggcccttcc ggtttgtccg cgaaacgcgc
    10081 cacgatgttg gtaagctcct gatccatcat cacataacgc ccgatggatg ggtaatgctg
    10141 gaccgaaagc tcgccgatgg aagggaggct tacgacgggg cccgcggagg atttgggatt
    10201 gacccattgt tggctcgtgg catcgaaaac ctcaaagccg ccaggggtat ccagatcgct
    10261 cagccgtttg cgagccaggt gaacggagct ggcgcggtat ttccccgtgc catacaggta
    10321 aagataaggt tcatcaacca acgccgcgat attgatgaac tttccccgaa gaggaccctg
    10381 ggaatcaaag cgctgatcca cttgatataa aatccggtaa tccggcttgc ctcccgtcga
    10441 tggatgctcc cacttggcaa ggtacgagcc gcgcatctcg aaagggttca gattgaccgt
    10501 cgtgtaaaag acataaatcg acccaccata ggagaaagct cccgaaggaa cttcgaagtc
    10561 tcccggcaaa tgcggaaaca tattggcacc ccggggcccc gcgggattgt ggatgtaatt
    10621 ggaaatcggc tcgccagcag gcggctgcat gctcgctccc gcgaaatccc cttccacatt
    10681 acctgttttc ccgcccgtga gcagaaaacg aaggttggaa cataacagac tcggattctg
    10741 ggctacagac gaataaggaa ctgccgaata tcccacggca tcgggcaacg attccggacc
    10801 caagggccaa atatgctgcg ctcccgccgt atcaccgaaa aatatatata aatcgtttcc
    10861 atgggcaacg ctgatgccca aatccgtgcc gcgtaaattg gcccgataat gcgtgttgtt
    10921 cggtgtggga tcatcgatgt tccggtcgct gatgagcttg catagtacct caagcctctt
    10981 gagcttaatg gcctcactat aggggttccc cccaccgctg cctcccgtct cttccggatc
    11041 atccccccct gaaccacccg tggcagaacc agaacccccc gttcctccag aaccccccgt
    11101 tcctccagac ccgcctttcc ccgaactcga gccgcctttc cccgaactcg agccgccttt
    11161 ccc
//
LOCUS       DAOSOR010000131        11139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5836_length_11139_cov_4.252887, whole genome shotgun sequence.
ACCESSION   DAOSOR010000131 DAOSOR010000000
VERSION     DAOSOR010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11139
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5836_length_11139_cov_4.252887"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1644
                     /locus_tag="PK710_09195"
     CDS             <1..1644
                     /locus_tag="PK710_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09934.1"
                     /translation="TKSIGVNETTVRFLHALVGASSLDMGVGSADELPADIISPVFSS
                     VGFAGIAEAGNTPTGRIDENGYLKLEVSGGVIAFAVANNGSTASFMVKSDKYDRGVSY
                     SFFAAGRKGDRKFPPEIMICDEMKADGIYTRCGGLPLTMTVDAANLQLAGAFGPYDKE
                     RIPAIQSAIASLPSDVVCVHESWGGSLRDGIAAAAASKFPHKASFNYDLDSLVDDATD
                     QSGNDPPEWTEAPCAQSDTKIAAALDCIKANCVEPLGSEDGIPKVPLSSCLTSKCMAS
                     MLPLLGPDSNDNACYSCLFGNLAAFETVGYIRDQCSHNPKARFAHRGDSGVMVLSKHP
                     VKNPESWVLASTEWRVNIVRAPVSLPNGAVVDVYCTQLTTPADSITRPYTGQYGDGKK
                     GLEAWRSELYLQAHKLVSYVQSKSETRGRKAVVVGSIYSGPEYYDGEKKILEAVNPEA
                     FNTVSTAFPLAVPASYVPVCTLCNDNLILAEPGSTPSGASTWQTFVFLSGIPITAVEN
                     AERFWTEPALEVESVDTGNKYAIPLSTHYGFRSIIRVLK"
     gene            1662..3092
                     /locus_tag="PK710_09200"
     CDS             1662..3092
                     /locus_tag="PK710_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09935.1"
                     /translation="MKTATRAWATCLFLFGGLSAYGCGDDDAQAPAGGSGAHSATGGA
                     AGSGGSVATGGGQNVGGAGTGAEAGTGATGGAQSGGGTGGSGATGGSGGTETGGAAGQ
                     GAGGTGGDQGGGGCAFVPGGDDFTRKIVVAHNRGPGGVESNVYEVLELSESGQISRPG
                     VTFEMGSGEGEIVFTTGGAIGIAIQGQGKDRSLGVFRFDDNGDLFVVNPAIHPSFYPH
                     KIIIPPGNNGDAYVLDAEWVEHGGGVYKISFGCEGQYYEMGKVLDAKLPNTMLLLDDD
                     RVFLSSTDVLGSDPSMEAHLIDLLSPPVRVASAEAFGDDDAIIVSAARTEDGKYVLLG
                     DDSMFGGERVGVVRIDGDSLSYVQTIPSVPAPVSIVPSPNNDVVLVVSEYPDDIHVLN
                     YDPSNSSKPFSLRGKLAYVGQKPSLPSTAVMIERGSLKGHVLVADLYGIRQIQFTAGG
                     QGVKDLGLYKLGESFPDTISALGIQK"
     gene            complement(3179..4831)
                     /locus_tag="PK710_09205"
     CDS             complement(3179..4831)
                     /locus_tag="PK710_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT09936.1"
                     /translation="MTPKPAFRSTITQALADTVIDESRSDPGSSPRPTLDVSKLPEVG
                     DMVGSMYRLIRVLGQGTFGKVYVAERVDVPEHQVALKITLREAYSGRDVERELVMLAA
                     AGHPHMVQLKDHGTTAEYVWFTMPVYEGETLAKRLQRGTLSLKEAHEIFVPIARSLDA
                     LHRSGLRHQDLKPDNVFLAQFGGRIHPIILDLGVAAERTSKFVAGTILFASPEQTLAL
                     THSEEDVPLSEKMDTYGLAATLLLSLVGTEYFPGSNAGTYQEMESAQHLRATHPLASE
                     ALPHLKGNARKLLEKALCAWFTLDPSQRPSMAEMADGLDVLLEQEREEKKAEALARTR
                     QKAKLLRTRLAVVALMVLALGFLGAAYSKRETLQLANELEQARAEEKQSFDNLNTCNA
                     AQKIVQRRLARCEETLQEEASLRREQEKLRKSSDESKDECTKQLMTFDTKVKTCEDDA
                     DAAAKACSSEREKLVAEQQKQVKELTEERDNQRQLAEECQGQVDKLRQGNTRCEADLA
                     VCQLGRKNRDNPYTDPLPSASTPSSTPPPLPPPPAEDPIIWE"
     gene            5017..6891
                     /locus_tag="PK710_09210"
     CDS             5017..6891
                     /locus_tag="PK710_09210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT09937.1"
                     /translation="MRERIRSSDIGPSDNAELQQSTALGAKGIFRRFVFVLVVFEVIV
                     GVAVLTYDILDTTASMNDKLESLATVGMQVVQTMRTDSPETSNADLLCQAAKVTGFPM
                     GLIAHGGSLAFATDPILYDAIDKTYPRGPIEPSRRFAIRDDLGDVSGGWFVQPFSNQY
                     YLLIVVPHLPESEGKMLHFTIRAGVLGLSLAASVLVMLGTANWMLRHPLDRLVRALTS
                     ALNRDIERRRIAEQVAIDARLDAEAHLAFLDNLINASDQVGIVAADEDGYVQLLNRAA
                     EAILGYSEAEAVGKMTLTELLSHSSQDSMHEVPLRSLMTLQESEMFVSDRHGVERLVS
                     VNFSDIKDVEGKDKGHLMVFIDVTERKRLEVELQINEMQLVQSAKLAGLGEMATGVAH
                     ELNQPLNNIGLLASRVTRKLINHDHEHAFEIEKLQKIQGQVQRAGKIIDQLRAFGRPT
                     VLHVTDFPIRQPVEAVLDLLGEQLSGRGIEVVVDIPDGLPNVQADESQLEQVLINLLN
                     NARDALSDSGVSPPSPRIRLKAEAEILPGDGELRICLHVQDNGPGMGDDVGRRVFEPF
                     FTTKEVGKGTGLGLSISYSLVRGFGGTLAFRSRPGEGTTFSILLKTVAPPASLPSENP
                     "
     gene            6888..7793
                     /locus_tag="PK710_09215"
     CDS             6888..7793
                     /locus_tag="PK710_09215"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT09938.1"
                     /translation="MKQSLKVLLIDDEALVRDELGGLLMDEGYQLMTAPDGESGLASF
                     RDNAPDMVITDVRMPRRDGLSVAMTIRQQAPTTPITVITGHGNEAMVLEALRAGVTDF
                     IKKPVRFEDLINALERMEAALRLARTQGADLPAAVSVKERTWVYELENDLSAIPLFVD
                     VLLREVGALAHPRVASELSLALRELLINAIEHGNLQLTYEEKSHALEIGNLSRIIEER
                     RLQPTLKQRKTILRIARRGPSLSVRIIDEGNGFDWKSLPDPTEPSYLLAEHGRGVLLA
                     RLAVDELSYNEIGNEVTFTKLLPEA"
     gene            complement(7820..9037)
                     /locus_tag="PK710_09220"
     CDS             complement(7820..9037)
                     /locus_tag="PK710_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOT09939.1"
                     /translation="MIMQIGLDRLVSMTGLIRSLRAGRVGLLAHPASVDGNLEHVSRV
                     LRRLGIVPRRIFGPEHGYGGEAQDMVGVEHATDTISGAPIVSLYGQSYEDLVPRREQV
                     EDLDWIIADLSDVGSRYYTFVWTMVLMVRAAHAAGVGVVLLDRPNPIGGERLEGKVQQ
                     PGYLSFVGLEPIPVRHGLTAGELVAEMASRDGIGIGPGEPLDVVALVGWEGRKGAECW
                     ERPFVMPSPNMPTADTAVVYPGGCLLEGTNLSEGRGTTRPFEICGAPWLDGVRMAGAL
                     HALPLEGFVARPLTFQPTFHKYAGQICGGVQIHVTDRDRFRPMATYVALVALAAYHAG
                     GFFRFRTERYEFVEGIPAIDLLSGSASLREGIEAGKEPMALIDEVCSVDESDELVHQR
                     AMERVMQAEAKRK"
     gene            9085..10449
                     /locus_tag="PK710_09225"
     CDS             9085..10449
                     /locus_tag="PK710_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOT09940.1"
                     /translation="MRRVLVVDDEENIRLVLRTVLRRSGYEVETASNGEEALKLVESF
                     GPDFVLTDARMPKMGGLDLLSTLRAKQSDATVIVMSAYGNVDVALEAIKAGAYDYVQK
                     PFKNDEIVLALRKAEEREALRRENRALREEIRKEYRFEDLLAKSASMQEVFRTVAKVA
                     DFKTTVLISGESGVGKELVARAIHNRSARAKSRFVAVNCGAIPETLLESELFGHRKGA
                     FTDASADRRGLFEEADGGTLFLDEVGELPLPLQVKLLRVLQDETIRRLGDNRDVRVDV
                     RIVAATHRDLVAEIKANRFREDLFYRLNVLPIKLPPLRERRDDIPLLLDHFLSRNNVR
                     LGTTVRGVDPEARRLLLEYAWPGNVRELENSIERAMVLAEGEVINANDLPERIRESRD
                     PIKMQLSSGELSIKKTTRIIEEVLIRRALQKTKGNRTRAASILEISHRALLYKIKEYG
                     LTDA"
     gene            10649..>11139
                     /locus_tag="PK710_09230"
     CDS             10649..>11139
                     /locus_tag="PK710_09230"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOT09941.1"
                     /translation="MTITRELVEKALDGVLEPTTGKPITELGSITEIAVTGDDVQLSF
                     RLPVHGATSQEKLRAPIVEAITAAGAKQVKIQWSTHVTGRDIGKDDPVPSVANVIVVM
                     SGKGGVGKSTVSANMALALAKAGARVGLLDADMYGPSVPVMLGTHARPTSLDGNSLEP
                     VER"
BASE COUNT         2493 a         3382 c         2905 g         2359 t
ORIGIN      
        1 acgaagtcga tcggtgtgaa tgagacgacc gtgcggttcc tgcacgcttt ggtcggtgct
       61 tcctcgctcg atatgggggt gggcagcgct gatgaactcc ccgccgacat tatttcgcca
      121 gttttctctt ccgtggggtt tgcgggtatc gcggaagcgg gcaacacacc aacaggtcgt
      181 atcgacgaaa acggttacct caaacttgaa gtcagcggag gcgtgatcgc gtttgctgta
      241 gctaataatg gctcgacggc aagcttcatg gtcaaatcgg ataagtacga ccggggggtt
      301 tcctattcgt ttttcgcggc tggcagaaaa ggagaccgaa aatttccgcc cgaaatcatg
      361 atctgcgatg agatgaaggc ggatgggatt tacacccgtt gtggtggctt gcccctcacc
      421 atgaccgtcg acgccgccaa cctgcaacta gcgggagcgt tcggtcccta tgacaaagag
      481 cgcattcccg cgatacagtc cgcgatcgca agcctcccct ccgatgtcgt atgtgttcat
      541 gagtcatggg gaggatcgtt gcgtgatggc atcgccgcgg ctgctgcgtc caagtttccc
      601 cataaggcat cgttcaatta tgaccttgat agccttgtgg atgatgcgac ggatcaatcg
      661 ggcaacgatc cccctgagtg gacggaagca ccctgtgctc aatcggatac aaaaattgca
      721 gcggccctcg attgcatcaa agccaattgt gtcgagccgt taggaagcga agacggtatc
      781 cccaaggtcc cgctttcgtc ctgtttgacc tccaagtgca tggcctccat gcttccgttg
      841 ttggggcccg actctaacga taatgcctgc tactcgtgct tgtttggtaa cctcgctgcc
      901 ttcgaaaccg taggctatat tcgagatcag tgcagccaca atcccaaagc gaggttcgct
      961 caccgaggag attctggggt gatggtgctc tcgaagcatc ctgtaaaaaa tccagaatct
     1021 tgggtccttg cctccacgga gtggcgagtc aacatcgtgc gtgccccggt ttctcttccc
     1081 aacggtgccg tggtcgatgt gtattgtacc caactcacca cgcccgccga tagcatcaca
     1141 cggccctata caggacagta cggcgacgga aagaaagggc tcgaggcttg gcgttccgaa
     1201 ctgtatctgc aagcccacaa actcgtgtcc tatgtgcaaa gcaaatccga aactcgaggc
     1261 agaaaggctg tcgtcgttgg ttcgatttac tccgggcccg agtattacga cggcgagaaa
     1321 aagattctgg aagcggttaa tcccgaggct ttcaataccg tgtcgactgc ctttccattg
     1381 gcggtccccg cttcctatgt tcccgtctgc acgctgtgca atgacaacct cattttggcg
     1441 gaacccggtt ccaccccctc cggagccagc acctggcaga cgttcgtctt cctcagtgga
     1501 atcccaatca ccgccgtcga aaacgccgaa cggttctgga ccgaacccgc cctcgaggtg
     1561 gaatctgtcg ataccggtaa caaatacgcg attcctttgt cgacgcacta cggtttccgt
     1621 tccatcattc gtgtactcaa atagttgaaa aggacagagc gatgaaaact gccacccgtg
     1681 cctgggcgac gtgtctgttt ttgtttgggg gactatccgc atacggatgt ggagacgatg
     1741 atgcgcaagc gcctgctggc ggctcaggag cacattcggc caccggtgga gctgccggca
     1801 gtgggggaag cgtagccacg ggaggcgggc agaacgtggg aggcgctggg accggtgcag
     1861 aagccggcac cggagccact gggggcgcgc aatccggcgg cggaaccggc ggttcaggcg
     1921 caaccggcgg ttcaggcgga accgaaacag gcggcgctgc cggacaggga gcagggggaa
     1981 ccggcggaga ccaaggcggt ggcggctgcg cattcgtgcc cggcggggac gacttcaccc
     2041 gaaaaattgt cgtcgctcat aaccgtggcc ctggcggtgt ggaatccaat gtatacgaag
     2101 ttctggaact atccgaatcg ggacagatct cgcgtccggg cgtgacgttc gaaatggggt
     2161 caggagaagg ggaaatcgtc ttcaccaccg gtggcgccat cggcatcgcc atccagggac
     2221 aaggaaaaga tcgttcgctc ggtgtattcc gtttcgacga taatggagat ttatttgtcg
     2281 tgaatcccgc gattcaccct tcgttttatc ctcacaaaat catcattcct cccggcaata
     2341 acggtgatgc ctatgtgctc gatgccgaat gggtcgagca tggcggtggt gtgtacaaaa
     2401 ttagctttgg ctgtgagggc cagtattacg aaatgggaaa agtcttggat gccaagctgc
     2461 ccaacacgat gctcttgctc gacgacgacc gcgtctttct gagcagtacc gacgtgctcg
     2521 gctcggatcc atccatggag gctcatctca tcgacttgct ttcccctccc gttcgagtcg
     2581 ctagcgcgga agctttcggt gacgatgatg ccatcatcgt atccgctgcg cgaaccgaag
     2641 atggaaaata tgtcctgctc ggtgatgact ccatgttcgg cggagaacgt gtcggtgtgg
     2701 ttcggatcga tggcgattcg ctatcttacg tgcaaaccat cccttccgtc cctgcccccg
     2761 tttccatcgt tccatcgccc aataacgatg tcgtgctcgt ggttagcgag tatcccgatg
     2821 atattcatgt gctgaattac gatccctcga attcctccaa accgttttcc ttgcgtggaa
     2881 agcttgcgta cgttggacaa aaaccatcct tgcccagtac cgcggtcatg atcgaacgcg
     2941 gctctctcaa aggtcatgtt ctcgtagcgg acctgtatgg aattcggcaa atccagttca
     3001 ccgcgggagg acaaggtgtg aaagaccttg ggctatacaa attgggcgaa tctttcccgg
     3061 ataccatcag cgccctcggc atacaaaaat agctgtagga aacgtcgatc gttgttccct
     3121 ttggttttgt cgctacctca ggcgcgtggt ggcgagcccc tgagccttgg taaaaacgct
     3181 actcccagat gatgggatcc tcagcaggcg gaggaggcaa gggaggcggc gtcgaggaag
     3241 gagtggatgc ggagggtagg ggatctgtat aaggattatc cctatttttt ctgccgagct
     3301 ggcatacagc caggtccgct tcgcatcggg tattgccctg tcgaagcttg tcgacttgac
     3361 cctggcattc ctccgccaat tgacgttgat tgtcccgttc ctccgtgagt tctttaacct
     3421 gtttttgttg ctccgcaaca agcttctcac gctcggaaga acaggctttg gctgccgcat
     3481 ccgcatcgtc ctcgcaagtt ttcaccttgg tgtcgaacgt catgagctgc ttggtgcatt
     3541 cgtctttcga ttcgtctgac gatttcctga gcttttcctg ttctcggcga aggctcgcct
     3601 cttcttgaag cgtctcctcg caccgagcta gacgacgttg cacgatcttt tgtgccgcgt
     3661 tacaggtatt caagttgtcg aaggattgct tttcctcggc tcgcgcttgc tctagttcgt
     3721 tggcaagctg aagcgtctct cgcttggaat aggctgcgcc gagaaatccc aacgccaaca
     3781 ccatcagcgc caccacggca agacgagtgc gaagaagctt ggctttctgt cgcgttcgcg
     3841 ctagcgcctc ggccttcttt tcttcgcgct cttgttcgag caatacatcc agaccgtcgg
     3901 ccatctccgc catcgaaggg cgttgggaag gatcgagcgt aaaccaagca cacaatgcct
     3961 tttcgagcag ttttctcgca ttgcccttca gatgaggcaa agcttcggag gctagagggt
     4021 gcgtcgcgcg aagatgctga gccgactcca tctcttgata ggttcccgcg ttgcttccgg
     4081 ggaagtattc cgtaccgacg agcgacagca gcaaggtggc agcgagcccg tacgtatcca
     4141 tcttttcgct caaaggcacg tcttcttcgc tgtgggtgag cgctagtgtt tgctctggag
     4201 aagcgaacag aatcgtgcct gccacgaact tggatgtacg ctccgccgca accccaagat
     4261 cgaggatgat ggggtggatt ctcccgccga attgggccag aaatacattg tcgggcttca
     4321 gatcttgatg gcgcaacccg gaccggtgca acgcatcgag gctacgcgca atcggaacga
     4381 atatctcgtg cgcctccttc agagaaagcg ttccgcgctg caaccgcttg gccaatgttt
     4441 ccccttcata aactggcatc gtaaaccaca cgtattcggc cgtggtccca tggtctttga
     4501 gctgaaccat gtggggatgc cccgcggccg ccagcatgac caattcacgc tccacatcgc
     4561 ggcctgagta cgcctctcgc aacgtgattt ttagcgcaac ctgatgctcc ggtacatcga
     4621 ctcgctccgc tacatatacc ttgccgaacg tcccctgacc gagcactcgg atgagccggt
     4681 acatgctgcc taccatgtcc cccacctcgg gcagtttcga cacgtctagc gtcggacgcg
     4741 gactcgaacc cgggtcggaa cgggactcgt cgatgacggt gtccgcgaga gcttgagtaa
     4801 tggtagaccg gaatgcaggt ttcggcgtca cggtggaaag gtaccctaat caaaaaaatc
     4861 tctgttctca aaagatgtcg acatgacgga tcgtcatgac ggcctcgagc tagataacgg
     4921 cttttctctt ctcggaattc ctgagatgaa tgcgccttcc tgtatgatgc cgtcgtacca
     4981 tccctttttg gatgaccatg atggttggat aaaacgatgc gagaacggat tcgaagcagt
     5041 gatatcggac cgagcgacaa cgcggaacta caacaatcca ccgcgctcgg cgcgaaaggt
     5101 atctttcgcc gtttcgtatt tgtcttggtc gtgttcgaag tgatcgtcgg cgtggccgtc
     5161 ctcacctacg acatcctcga taccaccgcc tcgatgaacg ataagctcga gtcgctggca
     5221 accgtaggta tgcaagtcgt tcaaaccatg cgtacggatt ctccggaaac ctcgaatgcc
     5281 gacttgctat gccaggctgc aaaagtgaca ggcttcccga tggggctcat cgcacacggt
     5341 ggtagcctgg cttttgccac ggaccccatc ctttacgacg ccatagacaa gacttatccc
     5401 cgcggaccca tcgagccttc tcgacgcttt gccattcgcg atgacctggg cgacgtgagc
     5461 ggtggctggt tcgtacaacc cttttccaat caatactact tgttgattgt cgttccccac
     5521 ctgcctgagt cggaaggcaa aatgctccac tttaccattc gcgcgggtgt gctcggcctg
     5581 agcctcgccg caagcgtgct cgtcatgctc ggaaccgcca attggatgct tcgacacccc
     5641 ctggaccgac tcgtgagagc gctcaccagc gccctgaatc gagacatcga acgacgccgc
     5701 atcgctgagc aggtcgcgat cgatgctcgc ctcgatgcag aagcccatct ggcttttctc
     5761 gacaacctta tcaatgcctc cgaccaagtg ggaatcgtgg ctgccgatga agacggctat
     5821 gtgcagttgc tcaatcgagc ggcggaggcc atcttgggat actcggaagc cgaagcggtc
     5881 ggaaaaatga cgctcacgga actgttgagc cattccagcc aagactcgat gcacgaagtg
     5941 cccctgcgct ctttgatgac gcttcaagaa agcgaaatgt ttgtctccga ccgccatggc
     6001 gtcgaacgcc tcgtctccgt caacttcagc gatatcaaag atgtggaagg caaagacaaa
     6061 ggacacctca tggtgttcat cgatgtcacc gaaagaaaac gcctggaagt cgaactgcaa
     6121 atcaacgaaa tgcaattggt tcagtccgcc aaactggccg gcttgggcga aatggccacc
     6181 ggagtcgccc acgagttgaa tcagcccctc aacaacatcg gcctgctcgc gtcacgcgtg
     6241 acccgaaagc tgatcaacca tgaccatgaa catgctttcg agatcgagaa actgcaaaaa
     6301 attcaaggac aagtccagcg ggcaggcaaa atcatcgacc agctcagagc cttcggacgg
     6361 cccaccgtat tgcatgtcac ggactttcct atccgtcagc ccgtcgaggc cgtcttagac
     6421 ttgctcggag aacaactcag cggccgcggc atcgaagtcg ttgtcgatat tcccgatggt
     6481 ctccctaacg ttcaggccga tgaatcccag ctagaacagg ttctcatcaa tctgctgaac
     6541 aatgcccgcg atgccctcag tgactccggt gtttccccgc cctctccacg aatccgcctc
     6601 aaagccgaag cagaaatcct tcccggtgat ggcgaacttc gaatctgcct ccatgtgcaa
     6661 gataatggcc caggcatggg ggatgatgtg ggacgacgag ttttcgaacc ctttttcact
     6721 accaaagaag tgggaaaagg cactggcctg ggcctgtcca tcagctatag cctcgttcgt
     6781 ggtttcggcg gaacccttgc cttccgatcg cggccaggag aaggaaccac cttctccatc
     6841 ctgctcaaaa ccgtagcacc tcccgcgagc ttgccgtcgg agaacccatg aaacaatctc
     6901 taaaagtcct gctcattgac gacgaagctc tcgttcgcga cgaactcggt ggcctgctga
     6961 tggacgaagg ctatcaactc atgaccgccc ccgacggcga gtccggcttg gcaagctttc
     7021 gagataacgc tccggatatg gtgattactg atgttcgtat gccaaggaga gacggcctgt
     7081 cggtggccat gaccattcgg caacaagcac cgacaacacc catcaccgtc atcaccggcc
     7141 acggcaacga ggcaatggtg ctggaagcgc tgcgcgccgg tgtcaccgat ttcatcaaaa
     7201 aacccgtccg attcgaagac ctgatcaatg ccttggagag aatggaagca gccctgcgcc
     7261 tcgctcgaac ccaaggcgcc gacctgcccg ccgccgtctc cgtcaaagaa cgaacctggg
     7321 tttatgaatt agaaaatgac ttatccgcca ttcctctttt tgtcgatgtc ctgctgcgtg
     7381 aagtcggcgc cctggcacac cctcgcgtgg ccagcgaact gagcctcgca ctgcgagaac
     7441 tgctcatcaa cgccatcgaa catggaaacc tgcaactaac ctacgaagaa aaatcccacg
     7501 ccctggaaat tggaaatcta tcccggatca tcgaagaaag aagactccaa cctacgctaa
     7561 aacaaagaaa aactatcctc cgaatcgctc gccgcggacc gtcgctctcc gtgcgaatca
     7621 tcgatgaagg aaacggtttc gattggaagt cactccccga ccctaccgaa ccttcctacc
     7681 tgctcgctga acacggaaga ggtgtgctgc tcgctcgcct ggcggtcgat gaattgtcct
     7741 ataacgaaat aggcaacgag gtgaccttca ccaaactgct gccagaggct tgacccgacc
     7801 cctgcgcatc accccgcctt cacttccgct tcgcctctgc ctgcatgaca cgctccatcg
     7861 ctcgctgatg caccaattca tccgattcat cgacgctaca tacctcatcg atgagtgcca
     7921 tcggctcttt tcccgcttcg atgccttctc tcagactcgc cgagccactg agcagatcga
     7981 tggccggtat tccctccacg aactcatatc gctccgtgcg aaaacgaaag aacccgcccg
     8041 catggtacgc cgctagcgcc accaaagcca cgtaggtcgc catcggacga aaccgatcgc
     8101 ggtccgtgac atgaatctgc acccccccac atatctgtcc cgcatactta tgaaacgtcg
     8161 gctgaaacgt cagcggacgc gctacaaacc cctccaacgg cagcgcatgc aaggctcccg
     8221 ccattcttac cccatcgagc caaggtgctc cgcatatctc aaacggcctc gtcgttcccc
     8281 gaccctccga cagattcgtc ccctccaaca aacatccccc cggatatacc accgccgtat
     8341 ccgccgtggg catgttcggc gatggcatga caaacggtcg ttcccaacac tccgccccct
     8401 ttcttccctc ccaccccacc aacgccacca catcgagcgg ttccccagga cctatcccta
     8461 tcccatcgcg actcgccatc tccgccacga gttccccagc cgtcagccca tgacgtactg
     8521 gaattggttc caaacccacg aaagataaat agccgggttg ttgaaccttt ccttccaacc
     8581 gctcgccacc aatgggattg ggacgatcta acaacacgac cccaacaccc gcagcatgag
     8641 ccgcccgcac catcaggacc atcgtccata caaatgtata ataccgactc cctacatcgc
     8701 tcaagtccgc gataatccaa tctaaatcct cgacctgctc gcgtcgcggc accaagtcct
     8761 catacgattg cccgtaaagc gatacgatcg gcgcgcccga aatcgtgtcc gtcgcatgct
     8821 ccacgcccac catgtcctgc gcttcacccc catagccatg ctccggccca aatatgcggc
     8881 gcggtacgat cccaagtcgg cgaagcaccc gactcacatg ctccaaattc ccatccacac
     8941 tagccggatg cgccaataac cccacacgac cagcgcgaag cgaacgaatc aaccctgtca
     9001 tcgaaaccaa acgatcgagc cctatctgca tgatcatgca aacattgcat attgtgagca
     9061 atggtgctaa aaaagttgca gaccatgcga cgagttctgg tagtcgatga tgaggagaat
     9121 attcgtctgg tgctccggac tgtgctgcgc cggagcggat acgaagtaga aaccgcttcg
     9181 aacggcgaag aagcgctgaa actagtcgaa agcttcgggc ccgatttcgt gctcaccgat
     9241 gctcgtatgc caaaaatggg aggattggac ctgctatcca ctcttcgcgc caagcagagc
     9301 gacgcgaccg tgattgtcat gagtgcttac ggcaatgtgg acgtggccct cgaggccatc
     9361 aaagccggtg cctatgacta tgtgcaaaaa cccttcaaaa acgatgagat cgtgttggcc
     9421 ctgcgcaaag ccgaggagcg tgaggccctg cgtcgggaaa accgcgctct gcgggaagag
     9481 attcgaaaag aatatcgatt cgaggacctg ctcgccaaaa gcgcctccat gcaagaagtg
     9541 tttcgtaccg tcgccaaagt cgccgacttc aaaactaccg tcctcatctc cggcgaaagc
     9601 ggtgtcggca aagaattggt cgcccgcgcc attcataacc gctccgctcg cgccaaaagc
     9661 cgtttcgtgg cggtcaactg cggcgctatc cccgaaacct tgctcgaaag cgaattgttc
     9721 ggccaccgca aaggggcctt caccgatgcc tccgcggacc gtcgcggact gttcgaggaa
     9781 gccgacggcg gaactctatt tctcgatgag gtgggagaac ttcccttgcc cttgcaagtc
     9841 aagctgctgc gcgtcttgca agacgaaacc attcgacgct tgggagacaa ccgcgatgtt
     9901 cgcgtggacg tacgcatcgt ggccgccacc catcgtgacc tcgttgccga aatcaaagcc
     9961 aatcgattcc gtgaagacct cttttaccgc ctcaacgtct tgcccatcaa actcccgcct
    10021 ctgcgggaac ggcgagacga tatccctcta ttgctagacc actttttgag tcgcaataac
    10081 gtgcgacttg gcaccaccgt gcggggggtc gaccccgaag ctcgacgctt gctgctcgaa
    10141 tatgcatggc cgggtaatgt gcgagaactc gaaaactcca tcgaacgcgc catggtgctc
    10201 gccgaagggg aggtgatcaa cgccaatgac cttcccgaac gcatccgcga gtcccgcgat
    10261 cccatcaaaa tgcagctttc cagcggagaa ctctccatca aaaaaaccac ccgtatcatc
    10321 gaagaggtcc tgattcgccg agcactgcaa aaaaccaaag gtaatcgcac tcgtgccgcc
    10381 agcatcctgg aaatcagcca ccgcgcgctg ctgtataaaa tcaaagaata cggcttgacc
    10441 gacgcttgag cgttgcgcca tcacgacaat cgtatcccat gccatcatgc ttctaccgtt
    10501 gacgaagggc atgtcgcgac ttaaggaacg ctcaaacacc accgagcctc acgctcttta
    10561 tggcctcgag gctcgagact catccccggg catgctgccc taacgcaaga cgcgcgatga
    10621 ctggcacgcg tcgtggagaa cccttatcat gaccatcact cgtgagctgg ttgaaaaagc
    10681 gctcgacggc gtcctagagc ccactaccgg caagcccatc accgaacttg gctcgatcac
    10741 cgaaatcgcc gtcaccggcg atgacgttca gctttccttt cgattgcccg ttcacggagc
    10801 cacctcgcag gaaaaactgc gcgctcctat cgtcgaggcc atcaccgccg ccggcgccaa
    10861 acaagtgaaa atccaatggt ccacccatgt cacgggtcgc gatatcggca aggatgaccc
    10921 tgtccccagt gtcgctaatg tcatcgtcgt catgagcgga aaaggcggcg tcggaaaaag
    10981 caccgtctcc gccaatatgg ccctcgcgct cgccaaagcc ggcgctcgcg tcggcctgct
    11041 cgacgccgac atgtacggcc ccagtgtccc tgtgatgctc ggaacccacg cacgccccac
    11101 atccctcgat ggcaatagcc tcgaacccgt cgaacgctt
//
LOCUS       DAOSOR010000132        11112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5852_length_11112_cov_4.692593, whole genome shotgun sequence.
ACCESSION   DAOSOR010000132 DAOSOR010000000
VERSION     DAOSOR010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11112
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5852_length_11112_cov_4.692593"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PK710_09235"
     CDS             <1..351
                     /locus_tag="PK710_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09942.1"
                     /translation="VSFGNLSDGEYQSPLTTFVFRLEAFPLFGWGGIYRNFGMSAEFG
                     AGRSTLQDKNENRQVADSGALSALGLGVFWEVFHTPPMAVAPYVGVQHNVSRWYDRND
                     VVGGLRIVLYGSQL"
     gene            complement(360..1055)
                     /locus_tag="PK710_09240"
     CDS             complement(360..1055)
                     /locus_tag="PK710_09240"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOT09943.1"
                     /translation="MTETLHDTQHPTTPESPTRPARKLSRALAGRLARYREILDNAQK
                     NGKSYVSSDSCGRELHVDGTLVRKDMASLGIIGKPHVGYPVAEVLAALERHVHEQTQR
                     AAVLVGVGNMGSALVRSGTLERHGVHLEAVFDTDQQKVGGTIGRHSVDPMHALEDRIQ
                     QCGAKLGVVTVPADAAQAVIDRMAAAGIRVIWNFAPIRPQIPTGVRVLNEHLAESVGR
                     LSRYLDESEMKFV"
     gene            complement(1434..2270)
                     /locus_tag="PK710_09245"
     CDS             complement(1434..2270)
                     /locus_tag="PK710_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09944.1"
                     /translation="MPRWIVIGSVMVVSAFLGGGLGGCGSNHEEMICDEAGCRICDAY
                     GCRPASPSVTGSGGTAGGAVDSGSPEPDVQSPPFCDPSTALCPCGGQSDCDEGYTCLD
                     GVCLVPCEFSSECGASRICVNGRCVVGCDTLVPCPDGYSCNAIGVCEIDSSTAGCGGA
                     SPCPGGLVCVEGVCRGTCETTAECASGEVCHAPTGTCIADPQPVRPCAKDPSVCGENQ
                     ACVEGYCRYPCTDDMSCKLIDARIPVCKDKICMSQAEAAPECTKKQDCPGGKDCVSNV
                     CK"
     gene            2496..3122
                     /locus_tag="PK710_09250"
     CDS             2496..3122
                     /locus_tag="PK710_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOT09945.1"
                     /translation="MICKRLRLTLSLAAFSILLLTAPLAAAQMKIAVVDMQRAVMETE
                     DGLRAQATLKKLFDRRQQELDARQDELKRSRDDIEKQAKVLSKEALERRMAEWQQSMA
                     ELQNVFMEFNKELQKEQNKMTQPILNRVVSVLRQLAAQDGYDAILERNAVPYVRTDLD
                     ITDRVIQRYNTGGAAPATSTPSTPTPAPPTTRPTPSAPAPRPAPTGSH"
     gene            3133..4089
                     /locus_tag="PK710_09255"
     CDS             3133..4089
                     /locus_tag="PK710_09255"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOT09946.1"
                     /translation="MTPPRDPHGIGVSFPCARSLAQLACDFGGDTTQPHVLVDNVAAP
                     KYANHRSLVPIFRASALKNLRDAAAVLVSPELVDKAHGYAPWRHPFAQFALAHLLTTL
                     APQRHPPTRSPHAIIENGVALGKDVTIGHGAVILAGAVLGDACIIEPRAVIYGSVRLG
                     QRVLIGAGAVIGRPGFGWASGPDRQRIRMPQLGGVEIEDDVEVGPLATVDAGTLKPTR
                     LRKGCKLDAHVHVGHNVEVGQYTLVAAQSGFAGSATIGNHVLIGGQVGVADHVTVGDC
                     TKIAGKSGVIGPIPAQSVVAGYPAVSRLRWLRAVATLMRGRS"
     gene            4086..4550
                     /gene="fabZ"
                     /locus_tag="PK710_09260"
     CDS             4086..4550
                     /gene="fabZ"
                     /locus_tag="PK710_09260"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOT09947.1"
                     /translation="MKTKAPSAQLDIHQILQLLPHRFPFVMVDRVDEVIAGKSIRGHK
                     CIAYNEPWFQGHFPQRPIMPGVLILECLAQIGAILAYASAPFDPTTNLMFFLGIDKVK
                     FRHTVVPGDRLDLSCHVLHQRSNAWKLNGEASVDGTLCAQGELLATIVDRAP"
     gene            4550..5395
                     /gene="lpxA"
                     /locus_tag="PK710_09265"
     CDS             4550..5395
                     /gene="lpxA"
                     /locus_tag="PK710_09265"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOT09948.1"
                     /translation="MPDIHPTAVIHPSARLHPGVSIGPLCVVGPHVEIGPDSKLIAHV
                     VIQGPTRMGARNTIHPFAALGGDPQHRDFAGEFTFLEIGDDNTIREHVTMHRGTKVGG
                     GTTRVGSRCLFMAGVHIAHDAQVGDHVTLTNATLLGGHVRIDPWAVSAGHVAVAPFTR
                     IGQSAFLAGNAMVEKDVPPFVIAAGDRATLRAINRVGLRRRELPEASIRAIDHAFRKF
                     FPREGKRMDPNDPQALSMGHDPLVQLFIEFLRTPSRQGIATRHRNKTRSQQARIDTQK
                     PITHD"
     gene            complement(5382..6023)
                     /locus_tag="PK710_09270"
     CDS             complement(5382..6023)
                     /locus_tag="PK710_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09949.1"
                     /translation="MKLTPLRSFVFVFASIACSSQTPPAVTPAPSQQPPSLSDTDWGR
                     FRSERQSLSIPLPQGKLWRIDDHSSQWLDAVHTPTQCRVRARTWVAAKAMSLDRCEQE
                     ARSYAPDLPAVVQGSVIDTFESSEWFAPGFVSRVVVGIKEADRSRGTAEGYVLVFGVE
                     AKRCLAMVFTATGAGPELGDRLNLGTKIAESTIVLSRLPGKGPLERVQVPNRE"
     gene            complement(6020..6775)
                     /locus_tag="PK710_09275"
     CDS             complement(6020..6775)
                     /locus_tag="PK710_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOT09950.1"
                     /translation="MGAKNKRKKKSEAKPSAKVEPPFFRPFSGLSSGGPRSEIEPTAP
                     VQAAKKSQRVMPSPEPEDVMTFERFMAGVQPLDATKASRVSKTQTDVESSFAKRAQSF
                     AKERELADQQALRQLHALVQDGSRFEVIDDGRRVEGRRRGTDGGMIRRMREGSLPVDA
                     SLDLGDVAAQQARSSLENFVRERRIRGDTVVIIHFGRSGHSVLRGEIAAWLSEGDAAK
                     HVAAFLSAPPEWGGENALCVLLANGAESHRGMR"
     gene            complement(6826..7296)
                     /locus_tag="PK710_09280"
     CDS             complement(6826..7296)
                     /locus_tag="PK710_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09951.1"
                     /translation="MINLPDPGLYRTTKPYPGYEQEIPEGALVYVGKNPDTGVTFVVR
                     PGSNRRNRWYWGEPTIPLRSTVWAQTLIPLRAEGFYTLPQDIDTGDGGRWIKNAIVQL
                     GYNREGQGIIFVGELHDNEERNVLLFSDRGMIVDDGLLDRLIWAPILPVSQPKE"
     gene            complement(7461..8627)
                     /locus_tag="PK710_09285"
     CDS             complement(7461..8627)
                     /locus_tag="PK710_09285"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT09952.1"
                     /translation="MSPFPSDDVMRGLLVVGAFVFSSCASPPSTKEPVRTVAMPGLQP
                     VESNDQGDSPPGNDVASARSPDPLPPLAVVGLARGTGTPADQALARGDDAYRNEQWDA
                     ASEAYLQAQRLAPTDPAPLVGQVRVALAKADAPTAHAADPQQPLVRQSVAKLRQARRL
                     DPSYGPALVELGQALLVLDESEEALSVLQAAVQQLPSNPDALSALGVALLANGRSQEA
                     VNALQKAVELDPGSIPRLTNLATAWLLQGETSRAVEVYERAVLLAPDDGRIHTDLGTA
                     YLALNDLQKAMRHLRRAVEIDPNRATFRSNLGYGLLSLGDTTGAIEQCRKALELDAKL
                     GSAWINLGTALARTGKRQQARQAFERAVALDPTDPRPRANLQELDEIERAGAAP"
     gene            complement(8608..10167)
                     /locus_tag="PK710_09290"
     CDS             complement(8608..10167)
                     /locus_tag="PK710_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HOT09953.1"
                     /translation="MSQFALRTQLPTYLVQERERIEKIARDYGLDPFDTIFEILAYDQ
                     MNEIAAYGGFPTRYPHWRFGMDYEQLSKSSEYGLSKIYEMVINNTPAYAYLLEGNSIT
                     DQKLVMAHVYAHVDFFKNNFAFRATDLDSSGNLTDPIARPEPFAPNRRWIDKMANHGA
                     RIRKHMERHGVTTVEQFIDTCLSLDNLVDPWRPFSGKPAPRDDEDRAIEVPRLRAKDY
                     MEPFINPSEYLEEQKKKLEQAQVKKRIFPESPSRDVLGFLVQHAPLETWERDVLEVIH
                     DEAYYFIPQMQTKIMNEGWATYWHSRMMTEKVLEASEIIDYADNASGVTATSGKQLNP
                     YKLGVELFRNIAERWDKGQFGKDWEECDDLDVKRHWNMRLGLGQKKIFEVRQIYNDVT
                     FIDEFLTPEFAIEHKLFSFSWSERNGRFEIDSREFAKVKNRLLFQLTNFGNPYIFVKD
                     ANYKNRSELLLLHRHEGVDLRADYARETLRALHRVWKRPVTISTIVDERPVELHFDGK
                     EHSSENVSLSE"
     gene            complement(10170..>11112)
                     /locus_tag="PK710_09295"
     CDS             complement(10170..>11112)
                     /locus_tag="PK710_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HOT09954.1"
                     /translation="GEKQTGGTGQGDGEPGDPVGAEQQEGEGTGKAGKDPGDHSLEVE
                     ISLDELAAILGEELELPAIENRGKSKIISAKDRYTGIRRVGPESLRHFKRTYREALKR
                     MVASGTFNPSRPVVVPIPDDKRYRSWKTHHEPVANAVIIYMMDVSGSMGDEQKEIVRT
                     ESFWIDTWLKSQYKGLETRYIIHDAAAREVDENTFFRTRESGGTMISSAYKLCSEIID
                     TSYPIDEWNIYPFHFSDGDNWSMDDTNVCLELLRNSLLPRVNMFAYGQVESPYGSGQF
                     IKDLKNAFSSDDRLVTSEIPDRDGIMASIKDFLGKGK"
BASE COUNT         2461 a         3370 c         2918 g         2363 t
ORIGIN      
        1 gtctcttttg gaaacctgtc cgacggagaa taccaaagcc cgctgacgac attcgtgttt
       61 cgcctcgaag catttccact gttcgggtgg ggcggcatct accgcaactt cgggatgtcc
      121 gcggaattcg gtgccggccg ttcgaccctg caagacaaaa atgaaaaccg acaagtcgct
      181 gattcaggag cgctttccgc cttgggactc ggtgtttttt gggaggtttt tcacactcct
      241 ccgatggctg tcgcgcctta tgtcggtgtg cagcacaacg tgagccgatg gtacgatcga
      301 aatgatgtcg tgggaggact gcgaatcgtg ctttatggca gtcagctctg aaaacccatt
      361 cagacgaatt tcatttctga ctcatcgaga tagcgggaaa gacgaccgac actctcggcc
      421 aagtgctcgt tgagcacacg aacccccgtg ggaatctgag gccgaatcgg cgcgaagttc
      481 caaatcacac ggatgcccgc tgccgccatc cggtcgatga ccgcctgcgc cgcatccgcg
      541 ggaaccgtca ccacaccgag cttggcacca cattgctgga tgcggtcctc gagcgcatgc
      601 atcggatcca ccgaatgtct gccaatcgtt ccacccactt tctgttgatc cgtatcgaat
      661 accgcttcga gatggacacc atggcgctcg agagtgcccg aacgcacgag agccgaaccc
      721 atgttgccca cgccgacaag gacagcggcg cgctgagtct gctcgtgaac gtgtcgctcg
      781 agagcggcaa ggacttcggc aacagggtag ccgacatgag gttttccaat gataccaagg
      841 ctggccatat cctttcggac cagggtgcca tcgacgtgga gttcacgtcc gcacgaatct
      901 gatgaaacgt aggatttccc gtttttctga gcgttatcga gaatttcccg ataacgggct
      961 agccgtccgg ccaacgcacg agatagtttt cgtgctggtc gagtcggcga ttcaggggtt
     1021 gtggggtgct gtgtgtcatg gagcgtctca gtcatcaagg cctccggttc cgcggttcat
     1081 tatcccctcg tcacgaatcc gctttccaag gaaccttagt gccgtttcga gacggatcaa
     1141 gcaattgcga catgcccccc gcgttcgtca cgaaatactc ccgcaccata ggcaagttat
     1201 tgaatttaca cgatatttcg tgtagcaaaa accatcacga acccgcttca cgatttcgtg
     1261 aacctgaccc caccgcgcat caattggatg gattcgcact tcgacaacaa cggatcaatg
     1321 caggcaaagc gctcgataca agcttcgata caagggtttt gtcacgaaaa aaagtggcgt
     1381 gaacgtcgcg aattgcagtt cctcattcgc acccgcgaac actgaatccc gcttcactta
     1441 cacacgttcg acacacaatc cttcccacca ggacagtctt gcttcttcgt acattcaggc
     1501 gctgcctccg cctggctcat gcatatctta tccttacaaa ccggaatccg agcatcaatg
     1561 agcttacacg acatatcatc cgtacacgga tagcggcaat atccctccac gcaagcttga
     1621 ttctcaccgc aaaccgaagg gtccttcgca catggcctga caggttgcgg gtccgcaatg
     1681 caggtgcccg taggcgcatg gcaaacctcg cccgatgcgc attccgcagt agtttcgcag
     1741 gttcctcgac ataccccctc cacacatacg agccccccgg ggcacggact tgcaccacca
     1801 catcctgccg tactcgagtc gatctcgcat acaccaatgg cgttacaact atacccatcc
     1861 ggacatggca ctaacgtgtc gcacccaacc acgcatcttc cgttgacaca gatgcgcgac
     1921 gctccacatt cggagctgaa ctcacacggc accaaacaca caccgtcgag acaagtatat
     1981 ccttcgtcgc aatcgctctg cccaccacag ggacaaaggg ccgtcgaagg atcgcagaag
     2041 gggggggact ggacatccgg ctcaggactc cccgaatcca ccgctccccc cgccgtccct
     2101 ccggaacccg tcaccgacgg tgacgcggga cgacagccat aagcatcgca aatgcgacac
     2161 cctgcctcat cacaaatcat ctcctcgtga ttgcttccac agcctcccag gcctccacca
     2221 aggaaagcac tcacaaccat taccgaacca atcactatcc accgaggcat tcgaacctcc
     2281 ttggggcacg cccgtccacc actctccaaa ggacatgcca aataggcggt ttccatttcc
     2341 tgacagattc tatcgtgcag cgccactccc cccaccccca cctgtgaaca tccctgctca
     2401 tagattgtaa tctctcattc acaccgagcg ccaacctcca aaacagacct tgccgtcgct
     2461 tgaccggtag ccccacgaag gctaggggtc cgagcatgat ctgcaaacgc ctacgcctca
     2521 ccctcagcct cgccgctttc tccatcttgc tgctgacagc tcccttggcc gccgcacaaa
     2581 tgaagatcgc cgtcgtcgac atgcaacgcg ccgtcatgga aaccgaagac ggcctgcgcg
     2641 cccaagcaac cctcaaaaag ctcttcgacc gacgacaaca agaactcgat gctcgacaag
     2701 atgaactgaa acggtcccgc gacgatatcg aaaaacaagc caaagtcctg tcgaaagaag
     2761 ccctcgaacg tcgcatggcc gagtggcagc agagcatggc ggaactgcaa aacgtattca
     2821 tggagttcaa caaggagctt caaaaagagc agaataagat gacgcaaccc atcctcaacc
     2881 gggtggtcag cgtgttgcgc cagctcgccg ctcaagatgg ctacgatgcc atcctcgaac
     2941 gcaatgccgt cccttacgtt cgaacggatc tcgatatcac cgaccgagtc atccaacgat
     3001 acaataccgg gggagccgcc cctgccacct ccacaccgtc aacccccacg ccagcccctc
     3061 ccaccacacg ccccacaccg tctgcccccg ccccacgccc agctcccact ggctctcact
     3121 gaccgccagg tcatgacacc cccacgcgat ccgcatggga tcggagtcag ctttccctgc
     3181 gcgcggtccc tggctcaatt ggcatgcgat tttggaggcg acaccacaca accgcatgtc
     3241 ctcgtggata atgtcgccgc accgaaatac gccaaccatc gctcgctcgt tcccatcttc
     3301 cgcgcaagcg ccctaaaaaa tctgcgcgat gccgccgcgg tactcgtgtc ccctgaactt
     3361 gtcgacaaag cccacggcta cgctccttgg agacacccgt ttgcccaatt cgccctggcc
     3421 cacctgctca cgacactggc cccgcagcgc catccgccca cgcgtagccc ccatgccatc
     3481 atcgaaaatg gcgtcgcttt gggaaaggac gtcaccatcg gccacggggc cgtcatcctc
     3541 gctggcgccg tgctcggcga cgcctgcatc atcgaaccca gagccgtaat ttatggctcc
     3601 gttcgtctcg gacaacgcgt gctcatcggc gctggtgccg tcatcggaag acctggattc
     3661 ggctgggcct caggcccaga taggcaacga atccgtatgc cacaactcgg gggcgtcgaa
     3721 atcgaagacg atgtcgaggt cggacctctg gctaccgtcg atgcgggcac cctcaaaccc
     3781 acacgcctcc gcaaaggatg caagctcgac gcccacgttc acgtcggcca taatgtggag
     3841 gttggacaat acaccttggt ggcagcacaa agcggtttcg cgggctccgc gaccattgga
     3901 aaccatgtgc tcatcggcgg gcaagtgggt gtcgccgatc atgtcacggt tggtgattgc
     3961 acaaaaattg ccgggaaatc gggtgtcatt ggtcccatcc ccgcccaaag cgtcgtggct
     4021 ggatacccgg ctgtcagtcg tttacgctgg ctccgcgctg tcgcaacatt gatgcgaggt
     4081 cgttcatgaa aaccaaagcc ccgtcggcgc aactcgacat ccatcaaatc cttcagctat
     4141 tgccgcaccg cttcccgttc gtcatggtcg accgcgtcga tgaggtcatt gccggcaaaa
     4201 gcatccgcgg ccacaaatgc attgcctata atgaaccctg gtttcaagga cactttcctc
     4261 agcgtcccat catgccgggc gtgctcattc tcgaatgcct cgcccaaatc ggtgccatcc
     4321 tcgcttacgc ttccgcaccc ttcgacccaa ccaccaactt gatgttcttc ctcgggatcg
     4381 acaaagtaaa attccgccat accgtcgtcc caggcgaccg cctcgacctg tcttgccacg
     4441 tgctgcacca acgctccaat gcctggaagc tcaacggcga agccagcgtc gatggcaccc
     4501 tttgcgccca aggagaacta ctcgcaacca tcgtcgaccg cgcgccctga tgcccgatat
     4561 ccaccccacc gctgtcattc acccctccgc aagactccac ccaggcgtct ccatcggacc
     4621 cctatgcgtc gtcggtcccc acgtcgaaat cggccccgac tcgaaactca tcgcacatgt
     4681 cgtcatccaa ggacccacac gcatgggcgc ccgcaatacc atccacccct tcgccgccct
     4741 ggggggagac ccgcaacatc gcgattttgc aggtgaattt acgtttttag agatcggtga
     4801 cgataacacc atccgagaac acgtcaccat gcatcgaggc accaaagtcg gaggaggaac
     4861 cacgcgcgtg gggtctcgat gcctgttcat ggctggagtt catatcgctc atgatgccca
     4921 ggtgggcgat catgtcacgc tcaccaatgc aacacttctt ggaggccatg tgcgtatcga
     4981 cccttgggcc gtttcggcag gtcatgtcgc cgtagcaccc ttcactcgca tcggacaatc
     5041 cgcatttctc gcgggcaacg cgatggtaga aaaagatgtt cccccctttg tcatcgccgc
     5101 gggagatcga gcaacactcc gcgcgatcaa tcgtgtggga cttcgacgcc gtgagctacc
     5161 ggaggcatcg attcgtgcca tcgatcacgc ttttcgcaag tttttcccac gggaaggcaa
     5221 aagaatggac ccaaacgatc cacaagcctt atcgatgggc cacgatcccc tcgttcaact
     5281 cttcatcgag tttttacgca caccaagccg tcagggcatc gcaactcgcc atcgtaacaa
     5341 aacgcgatcg caacaagcac gcatcgatac ccaaaaaccg atcactcacg attaggaact
     5401 tgcactcttt ccaagggacc tttgccaggc aaccgcgaca agacaatcgt actttccgca
     5461 atcttcgtcc ccagattcag tctatcgcca agctcaggac ccgctccggt cgccgtgaag
     5521 accatcgcaa ggcaacgttt ggcttccacg ccaaagacaa gcacataccc ttctgccgtc
     5581 ccacgactac ggtcagcttc cttgatgcca accacgacac gagagacaaa ccccggagca
     5641 aaccattccg aagactcgaa agtatcgatg accgatcctt gcacgacagc gggcaaatcg
     5701 ggagcatagg agcgagcttc ttgttcacat cgatccagac tcatcgcttt cgccgccacc
     5761 cacgtccggg cacgaacacg acattgggtg ggtgtatgca cggcatcgag ccactgtgac
     5821 gaatgatcgt cgattcgcca aagcttgcct tgaggcaaag gaatcgaaag gctttgtcgc
     5881 tcggaacgga atcgacccca atccgtatcc gaaaggcttg gaggctgttg cgacggggcc
     5941 ggagtgacag caggcggcgt ctgcgaagaa caagcgatgg aggcaaaaac gaaaacaaag
     6001 gatcgaagcg gggtgagctt catctcatac ctcgatggga ttccgcgccg ttggcgagaa
     6061 ggacacataa ggcgttctcc ccaccccatt cgggaggtgc agaaagaaag gcggccacgt
     6121 gtttggcggc atcaccttca ctcaaccatg cggcaatctc accacgcaac acggagtgac
     6181 cgctacgacc gaagtgaatg atcaccacgg tatctcctcg aatacgacgt tcacgaacga
     6241 agttctcgag gctcgatcga gcttgttgcg ccgctacgtc ccccaagtcc aacgaggcat
     6301 ccaccggaag actaccttcc cgcatgcgac ggatcatgcc accgtcagtt cctcgtcgtc
     6361 ggccctcgac ccgtcgcccg tcatcgatga cttcgaaacg gctgccatcc tgcacgaggg
     6421 catggagttg tcgaagcgct tgttgatcgg caagctcgcg ttctttggcg aaagattgag
     6481 cgcgtttcgc aaaactcgat tcgacgtccg tctgggtttt ggagacgcgc gaggcttttg
     6541 tcgcatcgag aggttgcacg ccagccatga atcgctcgaa ggtcatgaca tcttccggtt
     6601 ccggagatgg catgacccgt tgcgattttt ttgcggcttg tacgggtgcg gtcggctcga
     6661 tttcgcttcg cgggccgcca gacgaaaggc cggaaaatgg acggaagaac ggtggctcga
     6721 cctttgcgga tggcttggcc tcggattttt tcttgcgctt gttttttgcg cccatcgttc
     6781 ctcttgcaat cgcgacaaga cagggaaatc aacgaaggta tgggctcatt ctttgggttg
     6841 ggaaacgggt aggatagggg cccaaatcaa gcgatcgagc agtccatcat cgacgatcat
     6901 gcctcggtcg gaaaatagga ggacgttgcg ctcttcgttg tcatgaagtt caccgacgaa
     6961 gatgatccct tgaccttcgc ggttatatcc aagctgaaca atggcgtttt tgatccacct
     7021 tccgccatca ccggtatcga tgtcctgagg gagcgtatag aaaccttcgg cacgaagcgg
     7081 gatcaaggtt tgcgcccaca ccgtggatcg aagcgggatg gtaggttctc cccaatacca
     7141 gcgattgcga cggttggagc cgggtcgaac gacgaaggtg acgcccgtgt cgggattttt
     7201 gccgacatag accagagcgc cttctgggat ctcctgttcg tagcctggat agggtttggt
     7261 ggtgcgatac aggccagggt cgggaaggtt gatcatgcgt ggttcattcc ttattggaaa
     7321 gttcgttgtg ggttgcggga aacaccgggg ccgagggtcg acccatggtt agcggaagtt
     7381 aaccttccgg cacgagcgcg caagcgccca gcgttcgatc acaaaaaagg ctaacatatt
     7441 tcaaaggttt cgggttcctc tcagggcgcg gcccctgccc gttcgatctc gtcgagttct
     7501 tgaagattgg cacggggcct gggatcggtc gggtcaagag ccaccgcacg ttcaaacgcc
     7561 tgacgcgctt gttgccgctt tcccgttcgc gccagagccg tgccgagatt gatccatgca
     7621 cttcctagct tcgcatcgag ttcaagggcc tttcgacact gttcgatcgc accggtcgta
     7681 tcgcccaacg acagcagacc atagccaaga ttggagcgga aagttgcgcg attcgggtcg
     7741 atttcgaccg cgcgacgaag atgacgcatg gctttttgca ggtcgttgag cgcaagataa
     7801 gcggttccca gatccgtgtg aatccttccg tcatccgggg ctagcaaaac agctctttcg
     7861 tacacctcga cagcccgcga cgtttcccct tgaagaagcc atgcggtcgc caagttggtc
     7921 aggcgaggga tggagccagg atcgagttcg acggcttttt gcaaggcatt gaccgcttcc
     7981 tggctgcggc cgttggcgag caacgcgacg ccgagagccg aaagggcatc cgggttcgag
     8041 ggaagctgct gaacggcagc ttgcaaaacg gatagcgctt cctcggattc atcgagcacg
     8101 agcagggcct gaccaagttc gacgagggca gggccatacg atgggtcgag tcgtcgtgct
     8161 tgtctgagtt tcgccacgct ttggcgcacc aagggttgtt gtggatccgc ggcatgggcg
     8221 gtaggcgcat cggctttggc gagggcgacg cgaacttgac ctacaagggg agccggatcg
     8281 gtcggggcaa gacgctgcgc ctgaagatat gcctcgcttg cggcatccca ttgttcgttt
     8341 cgataagcat catcgcctcg cgccagagct tgatccgcgg gtgttcccgt accacgagcc
     8401 aagcccacga ccgcgagcgg cggaagcggg tcaggcgagc gagccgaagc gacatcattg
     8461 ccaggcggcg agtcgccctg atcgttgctc tcgacaggtt gtaaccctgg catggccacg
     8521 gttcgaacgg gctccttcgt cgatggaggc gacgcacaag acgaaaagac aaaagcgccg
     8581 acaaccaaca acccacgcat gacgtcatca ctcggaaagg gagacattct cggaactatg
     8641 ctccttgcca tcgaaatgga gttcgacggg acgttcatcc acaatggtgg agatggttac
     8701 cggtcgcttc cacacgcgat gcagagcccg aagcgtctcc ctggcgtaat ccgctcgaag
     8761 atcgacacct tcatgtcgat gcagcaacag cagttcactt cgatttttgt aattggcgtc
     8821 tttcacgaaa atgtacgggt tgccgaagtt ggtcaattga aacaggagcc tgtttttcac
     8881 tttggcgaat tcgcgggaat cgatttcgaa ccgtccattt cgttcggacc aagagaaaga
     8941 gaacaattta tgttcgatgg cgaattcggg ggtgagaaac tcatcgatga acgtgacgtc
     9001 gttgtagatc tggcgaacct cgaaaatttt tttctgtccc aatcccagac gcatgttcca
     9061 atgtcgtttg acatcgaggt cgtcgcactc ttcccagtct tttccgaact ggcctttgtc
     9121 ccaccgctcg gcgatgttgc gaaacagctc gaccccgagt ttgtaaggat tgagttgttt
     9181 tcccgaggtc gcggtgacac cggaagcatt gtcggcgtaa tcgatgatct cagaggcttc
     9241 gaggactttt tcggtcatca tgcgcgagtg ccagtaggtt gcccatcctt cattcatgat
     9301 cttggtctgc atctggggaa tgaagtaata ggcttcatca tgaatgacct cgagcacatc
     9361 gcgctcccag gtttcgagcg gagcgtgttg cacgaggaat ccaagcacgt cacgacttgg
     9421 tgattcggga aagatgcgtt tcttgacttg tgcttgctcg agcttttttt tctgttcttc
     9481 gaggtattcg gagggattga tgaacggctc catatagtct ttggcacgca aacgcggcac
     9541 ttcaatcgcc cgatcttcgt cgtctcgtgg tgccggtttg ccggaaaaag gtcgccaggg
     9601 atcgaccaaa ttatcgaggg acaaacaggt atcgatgaac tgctcgaccg tcgtgacgcc
     9661 atgtcgttcc atgtgctttc gaatgcgcgc cccatggttt gccatcttgt cgatccaacg
     9721 acgattgggc gcgaagggtt cgggtcgggc tatcggatcg gtgaggtttc cggatgaatc
     9781 gaggtctgtc gctcgaaaag cgaagttgtt cttgaaaaag tcgacgtggg catacacatg
     9841 cgccatgacc aatttttgat cggtgatgga gttgccttcg agcaggtacg catacgcggg
     9901 cgtgttgttg atcaccatct catagatttt cgacagccca tattcactgc ttttggacag
     9961 ttgctcgtag tccatgccga agcgccaatg cggataacgc gtgggaaaac cgccataagc
    10021 agcgatttca ttcatctgat cgtaagcgag gatctcgaag atggtgtcaa acggatccaa
    10081 gccgtagtct cgtgcgatct tttcgatacg ctcccgctcc tgaaccaaat acgtcggtag
    10141 ctgcgtacgg agagcaaact gactcatggc tactttccct tccccaaaaa gtctttgatg
    10201 ctcgccatga tgccatcgcg atccggaatc tcgctggtca cgagtcggtc gtcagacgaa
    10261 aacgcgtttt tgagatcttt gatgaattgt cccgaaccgt acggactttc cacctgacca
    10321 taggcgaaca tattcaccct cggcagcaaa gaattcctca acaattcgag gcatacgttg
    10381 gtgtcatcca tcgaccaatt gtcgccatcg gaaaagtgga aaggataaat gttccactca
    10441 tcgatgggat aactggtgtc gatgatctcg gaacagagtt tgtacgcgct agagatcatg
    10501 gtcccaccgg actcgcgggt gcgaaagaac gtgttttcat cgacttcccg cgccgcggca
    10561 tcgtgaatga tgtatcgcgt ctcgagccct ttgtattggc tcttgagcca ggtatcgatc
    10621 caaaaactct ccgtgcgaac gatctccttt tgctcgtcgc ccatcgatcc ggatacatcc
    10681 atcatgtaaa tgatgacggc gttggccacg ggctcatggt gtgttttcca ggagcggtaa
    10741 cgcttgtcat cgggaatggg cacgaccacg ggtcgagagg gattgaaagt accgcttgca
    10801 accattcgtt tgagagcctc gcgatacgtg cgcttgaagt ggcgcaaaga ttcgggccct
    10861 actcgtcgga taccggtata tcggtctttg gcgctgatga tcttgctttt gccacggttc
    10921 tcgatggcgg gaagttccaa ctcttccccc aaaatcgctg ccagttcgtc gagagaaatc
    10981 tccacttcga gcgagtgatc accagggtcc tttccggcct tgcccgtgcc ctcgccctct
    11041 tgttgctccg cgcccaccgg atcgccaggc tcaccatcac cttgacccgt tccccctgtt
    11101 tgcttttccc ca
//
LOCUS       DAOSOR010000133        11061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5885_length_11061_cov_4.822460, whole genome shotgun sequence.
ACCESSION   DAOSOR010000133 DAOSOR010000000
VERSION     DAOSOR010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 11061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11061
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5885_length_11061_cov_4.822460"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..827)
                     /locus_tag="PK710_09300"
     CDS             complement(219..827)
                     /locus_tag="PK710_09300"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOT09955.1"
                     /translation="MLLPINPEHPEPRKVRRAVDALERGEVIAYPTDTVYGIGCDLFN
                     KKAIDHIYQIRHLPKTQKLTFICPDLSDIAHYAIVDNRQYRLLRRVLPGPYTFILEAT
                     REVPKIVQSKRKTVGIRVPNHPVVQALVSELGRPIITSSAVMPGGEPFIDPEEIDDAF
                     QGLAMVLDGGAGGLEATTVVDLTSGEIEIVREGVGSIEELYG"
     gene            complement(912..1499)
                     /locus_tag="PK710_09305"
     CDS             complement(912..1499)
                     /locus_tag="PK710_09305"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOT09956.1"
                     /translation="MQSTALAPGLLLAAPPLGDPNFSKTVVLLAMHSQEGSLGWVING
                     TKVAPVGSLLKKAGLVPEGIELPETPSYDRHARVGGPVSPLSVWALYRRDDRLDHPEQ
                     IVLDNEWAGTGNRTVVEAIARGEGPREFRLVLGYAGWAPQQLDEEIRAGAWLPVAFNA
                     QIVFGQDDDALWERAYAKMVGISPLAFTAMRPGRA"
     gene            complement(1572..2984)
                     /locus_tag="PK710_09310"
     CDS             complement(1572..2984)
                     /locus_tag="PK710_09310"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT09957.1"
                     /translation="MQPGNPSEGASIRAVQNHVSSTAESERTEGASISSVWRGTILDS
                     IADGVFTVDEAWRITSFNRAAETITGYSRQQVMGRPCYEIFQTSLCQSNCALKKTMDT
                     GLPVINVAVEARGKGGEALFLSVSTAILQAPDGRRVGGVETFRDLSSIGVASRFSLEQ
                     SVLEGLVGRHPAMQGLADRLPDVAGSDAPVLVEGPEGSGKTLLTRSLHELSSRRLRPC
                     VRVCCSALPESLLAFELFGGRDHYGREAVGRVRLAAGGSLVLDEVSALSPGLQRQILR
                     LVRDQVFEPQAGAVRRADVRVLACSRTSLEPRVASGSFLPELFEHLRVVRMTMPSLME
                     RREDIPRLADHFLRVLRRRTGKAIRGVSEEAMSLLMSQEYPANVRDLENAIEHAFMVC
                     HGEVIEPHHWPDSMLRKAESYRLREPVGFSNPRQAAEAEVIRQVLERHRGNRLAAARE
                     LAMHRTTLWRKLKQYGMASD"
     gene            complement(3018..3809)
                     /locus_tag="PK710_09315"
     CDS             complement(3018..3809)
                     /locus_tag="PK710_09315"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOT09958.1"
                     /translation="MMRVALPLFGEDISPRFCFAREMIVIERHGQQEVSRRRVQLDDA
                     SWPERFSVLEEHRIDVLLCGGFPRRYLPYASRAGVRVIVGLAGRVEQVAEAFFAGRIE
                     DWIVGPCHGQREQEDMDHNDTDVVARKENMIEKQVVMVAVDGQGGLDAMVSAHFGHCA
                     GFVLAEVERGVIRASREVQNPHAQNHQPGMLPRFVQELGANVLLAGGMGQKAQTMLTR
                     SGIEVATGATGRARHAIEAFLRGDLRGFAPCGEHDHRASCGHHKD"
     gene            complement(3933..5300)
                     /locus_tag="PK710_09320"
     CDS             complement(3933..5300)
                     /locus_tag="PK710_09320"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT09959.1"
                     /translation="MKPPVDALYPLILDNIGEGVFSVDEDFHITSFNTEAERITGISR
                     DQAIGRKCYEVFRASICQTGCALAETVRSGKPIRNVRIDVLDANMDVVPICVSTAVLR
                     DEQGRLLGGVEIFRDISDVEALRSEVTGQKGFRDIIGTSVPMRRIFAMLPDVAASDAP
                     VLVTGASGTGKELVARAIHDLSRRHKGPFNQINCGALPDTLLESELFGYEKGAFTDAR
                     KSKPGRFVLADGGTLFLDEVGELSAAFQVKLLRALQEGEVQPLGSTRTIRVDVRVVAA
                     TNRDLARAVGERTFREDLFYRLRVIPIEIPPLAARPKDIPLLVDHFVKETARRTGKRI
                     EGVTAPALQALCRYDYPGNVRELKNIVERAFVFCREPRIDLSHLPEEVLVRETRESVP
                     PSDEVGLEESPTTQEVETLRKALEKHRWNRTATAKALGIGRNTLWRKMTKYGLLEVAD
                     TVPEP"
     gene            5388..7238
                     /locus_tag="PK710_09325"
     CDS             5388..7238
                     /locus_tag="PK710_09325"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT09960.1"
                     /translation="MGVSFGGRLDRKGMLALGKKDIFGDGILRLRDFGDWVRSQTRRE
                     RPKNCLNASLAEIPMHNRSSPWPRGPAYFTFGAICVLFFASMLHGGCLVTEHCYSNVD
                     CQVPKVCLADGTCGFQCVIDSDCGAGMGCIDYRCQPKSTQPIECPADMVNIAEAFCID
                     RFEASRPDATSTSEGMQSDKATSRAAVMPWQLFGPNGNETARLACLNAGKDLCTPQQW
                     QVACEGRAKTVYGYGNTYEPETCNGIDTYGLSGFHLLPTGSLSRCHSDWGVYDINGNL
                     WEHTLHGSDRTIRGGAYNCKDSQTLHRCDYIPGDWEPTARGFRCCLVPSAEIDAGTDA
                     SVDASADTSVDAPPDSATDSEVGCLDPDVVPSDVQQDVVSDVVQDVSADTQESGTEAS
                     TDAPVDSLIEVSSEGCPEDMVRIQHGKHDLCMDIYEASHIDATAVAQGMLMIATSRPG
                     VQPWQWADLAAARTGCQNAGKRLCRLDEWVVGCSGPLGTVYGYGNTYDPAICNGIDTY
                     CYCGTGTCAGLNVCPYPHCFSQASSEGGGPCGAMFHVDVTGAFPDCRSPWGVYDINGN
                     VWELTDTDDGQEHFRGGAYNCSDSEELHRCDHDGTWGPSARGFRCCKDPE"
     gene            7235..8803
                     /locus_tag="PK710_09330"
     CDS             7235..8803
                     /locus_tag="PK710_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09961.1"
                     /translation="MRLRVLAGILAWLVGLQGARAQQPSDTPRQVLDRAAQLQAKGDS
                     KGAIRELKAGRDAHRNHERIHHALALAYYQDGNAFWALKTLRDFEESHPPACASRALQ
                     VWIHVQQANFDLAQEILDEPGCDTPDFEAARVALLRATIASQHRDAASVNDYLEQARQ
                     ASRYYEEDFSLLQALTARFDPGRMPVISWRIDLAGGWTSNGLAGSPVDPADVGIDSQS
                     PLLQLDARLRVVIPASRAVRPVWEGQIRTLELFSATVRALSYRQPSFRAGILFGDRMP
                     RLLLAYGFDAVQVAGSDRYEQGPLWYSEGHRFDYELEVTDLFFAFGGAGHRTYRESGR
                     TRFEGEHGLAVVLPFADSLRLMTGISARWNNAANEAYDSQGGTALGQAQYRLPEGFEA
                     RLNGSLSYDRYPRSRGYFPGSLGEARSDMLVRVKPGLWSPSWSGFRVGLDYEFSHRDS
                     TAANYAYQDHRVLLHGVWVMDGDRFGVGLIPAENRQPLPHGVSSAQLETDETRIRDLM
                     QQDEAVKRGSSCLN"
     gene            8791..9420
                     /locus_tag="PK710_09335"
     CDS             8791..9420
                     /locus_tag="PK710_09335"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOT09962.1"
                     /translation="MSELNDTMPEKPTRGVLREIVRDAVIVTALSALVAVGVNALREE
                     GIRWVQQEEYDILVPCPEPVGEPEVMKGDDARMSDGKSLLLDVRSKQEYDAWHVSGAR
                     SQPFDWLGPPVDEEVKTVAKQVAASRAQRVIVYGDGDDPDSGKEWAKLLSGANIKNVF
                     YVEGGAPSLNPSLPKPEALPPLDDTTAPPIEALPDAQSADSEQNEKEAP"
     gene            9417..9905
                     /locus_tag="PK710_09340"
     CDS             9417..9905
                     /locus_tag="PK710_09340"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HOT09963.1"
                     /translation="MNRLLAWRGHAWISLVVRLYLGGVFLAACAHKILHPEVFAVDVA
                     TYQFLPLWSVNLFALVLPWIEAFTGVMLVLGIRVRAASLLIMGMMLSFMVALSWALVQ
                     GLDMSCGCFASQAAAEDDPISWVTLLRDAVWLVQSIYVLVVDRDAIGVERWLRKWRKR
                     HG"
     gene            9898..10749
                     /locus_tag="PK710_09345"
     CDS             9898..10749
                     /locus_tag="PK710_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOT09964.1"
                     /translation="MGSRLLLMMALSALVLIPRLALAQTPACDSLSGEKKALATALLN
                     EQHLYDCCDGTIQECLKEGKCSLATRLANQVCRMAAAGKDKGTIERALSQRATSMMSA
                     RAVPIDNSRAERAGEANAPVTLSLYLCARCPYCARLVPQLHKEITEGALKGKVKAYVR
                     EFPIRSHPGSTEGGMGMVAARRLGKGWPFLLHLYRIFDRFDPANMPKCAAEYGMDEEE
                     FRNLLNDEGVRRELTASKKEGVRNRVDATPTLFINGRKYTGDLSIETVVDVLAEEAER
                     VRAKATK"
     gene            10828..>11061
                     /locus_tag="PK710_09350"
     CDS             10828..>11061
                     /locus_tag="PK710_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09965.1"
                     /translation="MNAKMVFASFGVLLAANGLGCSSSAEALFDRKPVPGTDLDASAD
                     TGESGGSQNVGGSGGASTGGSGGGNYGGTGGSGG"
BASE COUNT         2267 a         3288 c         3196 g         2310 t
ORIGIN      
        1 gggtcgatac caccatcgac accatccaac ccagccccaa aacgacccat cgtttcgtcc
       61 tgttggctcg attcatcagc accatccacc catcctaagc aatgctttct gttctgtcga
      121 tgccactcat cgcaacgccg cggtctgcac gggctgtcaa ttcgggcaag tcgacacccc
      181 gccggatcga accgccgagc ctccggccat gaaacgggtt agccgtacaa ttcctcgatg
      241 gaaccaaccc cttcacgcac gatctcgatc tctcccgagg tcaaatccac cacggtcgtt
      301 gcctcgagtc ccccggcccc cccatccaat accatcgcaa gaccttgaaa tgcatcatcg
      361 atctcttccg gatcgatgaa gggttcccca ccgggcatca cggcggaact ggtaatgatc
      421 ggacgaccaa gctcactcac caacgcttgc accacggggt ggttgggtac ccgaattccc
      481 acggtttttc gcttggactg cacgatcttc ggcacctccc gtgtcgcttc caaaatgaac
      541 gtatacggtc caggcaatac gcgacgcagc agccgatact gacgattgtc cacgatggcg
      601 taatgggcga tatcgctcaa gtcggggcaa atgaacgtaa gcttctgcgt tttgggcagg
      661 tgtctgattt gatagatgtg atcgatcgcc ttcttgttga atagatcaca gccaatgccg
      721 taaaccgtat ccgtcgggta tgcgatgacc tcccctcgct ccaaggcatc gacggcgcgt
      781 cggactttgc gaggctcagg gtgttcggga ttgatcggca aaagcataac gaagccgggc
      841 tcgctcctag ccgacctcga cgatcttcac aaggtctgtg cgacgttcag gtccgtggcc
      901 ttcgttcatt ttcatgcgcg cccaggacgc atagccgtga aagcgagcgg tgaaatgcct
      961 accattttcg cgtatgcacg ctcccacaag gcgtcatcgt cttggccaaa tacgatttgc
     1021 gcgttgaacg ccacgggcag ccaagctccc gcgcggattt cctcatcgag ctgttgaggg
     1081 gcccatccgg catatccaag gaccagacgg aactcgcggg ggccttctcc cctcgcaatc
     1141 gcctcaacca ccgtgcgatt tcccgtccca gcccactcgt tgtccaaaac gatctgctcg
     1201 gggtggtcta aacgatcgtc ccgtcgatac agcgcccaga cgctcagcgg ggatacggga
     1261 cctccgacgc gcgcatgacg atcataggag ggtgtttctg gcaactcgat cccctcggga
     1321 acgagtcctg cttttttcag caaagagccc acgggcgcaa ccttcgtgcc gttgatcacc
     1381 cagccaagtg agccttcctg ggaatgcatg gcgagcagaa ccacggtttt agaaaagttg
     1441 ggatctccaa ggggtggagc ggccaacagc agacctggtg caagcgcggt ggattgcatc
     1501 ggaggaaacg tagtccaatt gcgtatcgaa cgctcgcata attccacacg cgtagtggaa
     1561 cggaacccgc ttcagtccga tgccatgccg tattgtttga gctttcgcca caaggtcgtg
     1621 cggtgcatgg caagttcgcg ggcagcggca agacgatttc cacggtgtcg ttcgagaacc
     1681 tgacgaatca cttctgcctc ggccgcttgc cttggattgg aaaaaccgac cggctcgcgc
     1741 aaccgatagg attcggcctt tcgcagcatc gagtccggcc aatgatgagg ttcgatcacc
     1801 tcaccgtgac agaccatgaa tgcatgctcg atcgcgtttt ccaggtcacg aacgttcgcg
     1861 gggtactcct gggacatcaa caacgacatc gcctcctcac tcacgccacg aatggctttt
     1921 cccgtccgac ggcgcagcac acgcagaaaa tggtcggcca gtcggggaat gtcttcacga
     1981 cgttccatca gcgatggcat cgtcatccgc acgacccgca ggtgttcgaa tagttcagga
     2041 agaaaggatc cggaggccac ccgtggctcg agactcgttc tcgaacaggc cagtacccga
     2101 acatcggcac gccgaaccgc ccccgcctgc ggttcgaaaa cctgatcccg cacgagcctc
     2161 aagatttgtc gttgcaatcc gggcgacaga gccgacacct cgtcgagcac caggctgcca
     2221 cctgccgcga gccgaacccg gcctaccgcc tcccgaccgt agtggtcgcg tccaccgaaa
     2281 agctcgaagg ccaacaacga ctcaggtaac gcggagcaac aaacccgaac acaagggcgc
     2341 aaccgacgtg acgacaactc atgaagcgag cgcgtcagga gcgtttttcc acttccttcg
     2401 gggccttcca ccagcaccgg ggcatcgcta cccgccacat ccggcaaacg gtccgccaag
     2461 ccttgcattg ccggatgccg tcccaccaag ccctccaaca cgctctgctc caaagaaaac
     2521 cgcgaggcaa cgcctatcga cgacaagtct cgaaacgttt ccacgccacc gacccgtcgc
     2581 ccgtccggcg cctgcagtat cgccgtgctt accgacaaaa acagcgcttc tccccctttg
     2641 ccccgggcct ccaccgccac gttgatgacc ggcaaccccg tgtccatggt ttttttcaaa
     2701 gcgcaattcg attggcaaag gcttgtctga aatatttcgt aacacggccg ccccatgacc
     2761 tgctgacggc tataaccggt gatggtttcg gcggcgcggt tgaaactcgt gatgcgccaa
     2821 gcctcatcga cggtgaatac cccatcggca atgctgtcga ggatggtgcc tctccacacc
     2881 gacgagatcg atgcaccctc ggtccgttcc gactcggctg tcgagcttac atgattttgt
     2941 acggctcgga tactagcgcc ttccgatgga ttacccggtt gcatcaatcc ctccaatgcc
     3001 aaaccacgac cgtggcgtta gtccttatgg tgcccacagg aagcccgatg gtcatgctca
     3061 ccacaaggcg caaatccacg caagtctccc cgcaaaaacg cttcgatggc atggcgcgca
     3121 cggccggtcg caccagtcgc tacctcgatg ccggaacgag tcaacatcgt ttgcgccttc
     3181 tgacccatac ctcccgccaa caaaacgttt gccccaagct cttgcacgaa tcggggaagc
     3241 atcccgggct gatggttttg cgcatgcggg ttttgcacct cgcgcgaagc gcgaatgacg
     3301 cctcgctcca cttccgccaa gacgaatccc gcgcaatgac caaaatgagc gctgaccatg
     3361 gcatccaatc cgccctggcc gtccacagct accataacca cttgtttttc aatcatattt
     3421 tccttcctcg ccacaacgtc ggtatcgttg tggtccatgt cttcctgctc gcgttgcccg
     3481 tgacatgggc ccacgatcca gtcttctatc cttcccgcaa agaacgcttc cgccacctgc
     3541 tccacacggc ccgccagacc cacgatgacc cgtactcccg cccgactcgc ataaggcaga
     3601 taacgccggg gaaatcctcc gcacaacaaa acgtcgatcc gatgctcctc gagcacggaa
     3661 aagcgctccg gccaggatgc gtcgtcgagc tgaacccgcc gacgcgagac ctcctgctgt
     3721 ccatgccttt cgatgacgat catttccctg gcaaaacaga atcggggaga gatgtcttcg
     3781 ccaaacaacg ggagcgccac tcgcatcatg ccgccacaaa gcgaccttcg tgccagcgcg
     3841 atgactctat tcttcgctgc aaagcaaaaa ccaacggttc aaacgcaggg gcgcatgacg
     3901 gaacaaaacg tctcatcgaa ggacagagtg tctcatggct ctggaacggt atccgccacc
     3961 tcgagcaagc cgtatttggt catcttccgc cacaaggtat tgcgcccaat tcccagagct
     4021 ttggcggtag ccgttcgatt ccaacggtgc ttttccaacg cttttcgcag tgtctccacc
     4081 tcttgggtcg tcggactttc ctccagccca acctcatccg agggtggaac cgactcccgc
     4141 gtctcgcgca ccaacacctc ttcgggcaaa tgagataagt cgattcgcgg ttcccgacaa
     4201 aacacgaacg cgcgctcgac gatatttttc aactcccgca cattgccggg atagtcgtaa
     4261 cggcaaagcg cctgcaaggc tggagcggtc accccctcga tccgtttgcc ggtgcgacgt
     4321 gccgtctctt tcacgaaatg gtccacgagc agagggatat cctttggtcg agctgcaagc
     4381 ggtggaattt caataggaat tacacgcagc cgataaaata gatcttctcg aaaggtgcgc
     4441 tcccctacgg cccgagccag gtcccgattg gtggccgcta ccacccgcac gtccacccgt
     4501 atcgtgcgtg tcgaccctaa gggctgcacc tccccttcct gcaaagcgcg caataatttg
     4561 acttggaatg ccgcggacag ctcccctacc tcatcgagaa atagcgttcc cccgtccgca
     4621 agcacgaatc gcccgggttt gctttttcgt gcgtccgtga aggccccttt ctcgtaacca
     4681 aatagctcgg actcgagcaa cgtatcgggc aacgccccgc aattgatctg gttgaaaggg
     4741 cctttgtggc gacgcgacaa gtcgtggatc gcccttgcca ccagttcttt tccagttcca
     4801 ctcgctccgg tcaccaacac cggagcatcg gatgcggcaa catcgggaag catcgcgaaa
     4861 atacgtcgca tcggcacgct cgttccaatg atgtcccgga atcctttctg tcccgtcacc
     4921 tcactgcgaa gcgcttccac atcggagata tcccgaaaaa tctcaacccc acccaggagc
     4981 cttccctgtt catcgcgcag caccgcggtg gacacgcaaa tcggaaccac gtccatattc
     5041 gcgtcgagca cgtcgatgcg cacgtttcga atcggtttgc ccgagcgaac cgtttccgca
     5101 agcgcgcatc ctgtctggca gatgctcgct cgaaatacct catagcactt gcgaccgatg
     5161 gcctgatccc tggaaatccc cgtgattctc tcagcttcgg tattgaacga cgtgatatgg
     5221 aagtcttcgt cgacggaaaa cacgccttct ccgatgttat cgaggatgag cgggtacagg
     5281 gcgtcgacag gtggtttcat gggcagaagt ggggcggtcc ctcgaaactt tagggtgtgg
     5341 ggcggtgaac ggcaagcgcg ggatcgttct gaggcatcga gtcggtgatg ggggtgtcct
     5401 tcggaggacg gctcgatcgg aagggcatgc ttgctctcgg aaaaaaggat attttcggcg
     5461 acggcatctt gcggctgcgg gacttcggtg attgggttag gtcgcagact cgtcgtgaaa
     5521 ggcccaagaa ttgcttaaac gcgagccttg cggagatccc catgcacaat cggagttcac
     5581 cttggccccg tggccccgcg tacttcacgt tcggagcaat ctgcgtcctt tttttcgctt
     5641 ccatgctcca cggtggctgc ctggtcaccg agcattgcta ttccaacgtg gactgccagg
     5701 ttccgaaggt atgtcttgcc gatggcacgt gtggatttca atgtgtcatc gactcggact
     5761 gtggggcggg tatggggtgc atcgattatc gttgtcagcc caagtcgact cagcccatcg
     5821 aatgcccggc ggatatggtc aacatcgcgg aagcgttttg catcgaccgt ttcgaagctt
     5881 cccggcctga cgccacatcg acatcggaag ggatgcagag cgacaaggcc acgtcacggg
     5941 ccgcggtgat gccttggcag cttttcggtc ccaacggcaa tgaaaccgct cggcttgcct
     6001 gcctcaacgc cggtaaggat ttatgcaccc cacaacagtg gcaggtcgcg tgcgaaggac
     6061 gcgccaaaac cgtgtacggc tacggaaaca cgtacgaacc ggaaacctgc aacggcatcg
     6121 atacctatgg gctcagcggc tttcatttgt tgcccaccgg atcgctttcg cgctgccaca
     6181 gtgattgggg ggtctacgat atcaatggca atttgtggga gcacacactg cacggctcag
     6241 acaggaccat tcgtggggga gcgtataact gcaaagactc ccaaactctt catcgctgcg
     6301 actacatccc gggcgattgg gaacccaccg cacgaggctt ccgatgttgc ctcgtaccct
     6361 cggcggaaat cgatgctgga accgatgcat cggtagacgc atcggcagac acatcggtag
     6421 acgccccccc ggactcggcc acggactccg aggttggctg cctagacccc gacgtcgtgc
     6481 cgtccgacgt gcagcaagat gtcgtttccg acgtcgtcca agacgtttcg gccgacacgc
     6541 aagaatcggg aaccgaagca tccaccgatg cgccggtcga ctcgctcatc gaggtgtctt
     6601 cggaagggtg tcccgaggac atggttcgaa ttcagcacgg aaaacacgat ttatgcatgg
     6661 atatttatga agcctcgcat atcgacgcca cggctgtagc ccaaggtatg ctcatgatcg
     6721 ccaccagtcg tccgggcgtg cagccctggc aatgggcgga ccttgctgcc gctcgtaccg
     6781 gctgccaaaa cgccggcaag cggctttgcc gtctcgacga atgggtcgtt ggatgttccg
     6841 ggcctttggg cacggtgtat ggctatggca atacgtacga cccggccatc tgcaacggta
     6901 tcgacaccta ttgctattgt ggcacgggaa cgtgtgccgg gttgaacgtc tgcccttacc
     6961 ctcattgttt cagccaggcc tcttcggagg gcggggggcc ctgtggcgcg atgttccacg
     7021 tggacgttac cggcgcattc cccgattgca ggagtccttg gggcgtttat gacatcaacg
     7081 gcaatgtatg ggagttgacg gacaccgacg acgggcagga gcattttcgt ggtggtgcct
     7141 ataattgctc ggattccgag gagttgcacc gatgtgatca cgatggaacc tggggccctt
     7201 ccgcgcgtgg gttccgttgc tgcaaggatc cggaatgaga ttacgcgttc tggctggaat
     7261 actggcatgg ttggtgggtc tgcagggggc gagagcccag cagccgtctg acacgccccg
     7321 gcaagtgctc gaccgtgcgg cacaacttca agccaaaggg gattcgaagg gcgcgatccg
     7381 agaactcaag gcagggcgcg acgctcatcg gaatcacgag cggattcatc atgcgctggc
     7441 tctggcgtat taccaggatg gcaacgcgtt ttgggcgctg aaaaccctcc gtgacttcga
     7501 ggaaagccat ccacccgcct gcgcctctcg cgccctacaa gtgtggatcc acgtccaaca
     7561 agccaacttc gatcttgccc aggagatatt ggatgagcct ggctgcgaca cgcctgattt
     7621 cgaggcggcc cgcgtggcct tgctgcgcgc gacgatcgcc tcgcaacatc gagacgccgc
     7681 ttcggtgaac gattatctcg aacaagcgcg tcaagcgagc cgatattacg aagaagattt
     7741 ctcgttgctg caagcgctga cggctcgttt cgatccggga cggatgcccg tgatttcctg
     7801 gcgaatcgac ctggcgggtg gttggacttc caacgggctt gcgggctcgc cggtggaccc
     7861 cgcggacgtg ggcatcgact cgcaatcacc cctgctgcag ctcgatgcgc gccttcgtgt
     7921 ggtgattccg gcctcgcggg cggtgcgacc ggtgtgggaa ggtcagattc gaacgctgga
     7981 gctattttcc gccacggttc gcgcccttag ctatcgccaa ccctcgtttc gcgcgggcat
     8041 tctcttcggt gaccgcatgc cccgattgct gctcgcctac ggtttcgacg ccgtgcaggt
     8101 tgcggggtcc gatcggtacg agcaggggcc cttatggtat tccgaaggcc atcgtttcga
     8161 ttatgaattg gaagtcacgg atttgttttt cgcttttggg ggcgcgggtc accgcacgta
     8221 tcgggaatcg ggtcgtacgc gattcgaagg ggagcatggc cttgcggtcg tgctaccctt
     8281 cgccgattct ttgcggctca tgaccggcat ttccgctcgg tggaacaatg cggccaacga
     8341 agcgtatgat tcgcaagggg gcactgcgct tggtcaggcg caatatcgac ttcccgaagg
     8401 tttcgaggcc aggctgaacg gctcgttatc gtacgaccgc tatccccgtt ctcgaggtta
     8461 cttcccaggc tccctgggag aggcgcgttc cgacatgctc gttcgggtga aaccggggtt
     8521 gtggtccccc tcctggagtg gattccgcgt ggggctcgat tacgaattca gccatcgtga
     8581 cagcacggcg gccaactacg cgtaccagga ccaccgcgtg ctcttgcatg gggtttgggt
     8641 gatggacggg gatcgattcg gggtcggtct gattcctgca gaaaacaggc agccccttcc
     8701 tcacggggtg tcttcggctc agctagaaac agacgagacg cggattcgag atttgatgca
     8761 acaagacgaa gcagtgaaac gaggttcgtc gtgtctgaac tgaacgatac gatgcctgaa
     8821 aaaccgacgc gtggggtctt gcgggagatt gttcgcgacg cggtcatcgt gacggcgctg
     8881 tcggcgctgg tggcagtagg agtaaacgcg ttgcgagagg aaggaatccg gtgggtgcag
     8941 caagaagagt acgacattct cgtgccttgt cctgagcccg taggcgaacc ggaggtcatg
     9001 aaaggcgatg atgcgcggat gagcgatggc aagtccttgt tgctcgatgt gcggtcgaag
     9061 caggagtacg acgcctggca cgtgtcgggg gctcgcagcc aacccttcga ttggttgggg
     9121 ccaccggtgg atgaagaagt caagacggtt gcgaagcaag tggcggcgag ccgtgcgcaa
     9181 cgagtgatcg tctacgggga tggcgacgac ccggattccg gcaaggagtg ggccaagcta
     9241 ttgtccggcg ccaatatcaa aaacgtattt tacgtggagg gaggggcacc ttcgctgaac
     9301 ccgagtttgc caaagccgga ggctctacca cccctcgatg atacgacggc tcctccgatc
     9361 gaagcgctgc ccgatgctca aagcgcggat tccgagcaga atgaaaagga ggcgccatga
     9421 atcgtctgct cgcttggcgc ggtcatgcat ggatttccct tgtcgtccgt ctatatcttg
     9481 gaggtgtttt tctcgcggca tgcgcgcaca aaatcctgca tcccgaagta tttgccgtcg
     9541 atgtggcgac ctaccagttc ttgccgctct ggtcggtgaa cctatttgcg ttggtgctgc
     9601 cgtggatcga agcgtttacg ggcgtgatgc tggtgttggg gatacgggtt cgtgcggcga
     9661 gcctgctcat catggggatg atgctttcct tcatggtggc gctatcctgg gcgttggttc
     9721 aggggctcga catgtcttgc ggatgttttg catcgcaagc ggctgcggaa gacgacccta
     9781 tttcgtgggt cacgttgttg cgcgacgccg tttggctcgt acagtctatc tacgtgctcg
     9841 tggtcgatcg ggatgcgatt ggcgtggagc gttggcttcg caagtggagg aaacgtcatg
     9901 ggtagccggt tattattgat gatggcgctc agcgcgctgg tgttgattcc gcgattggct
     9961 ttggcacaaa ctcctgcctg tgacagtttg agcggggaaa agaaggcgct ggcgacggca
    10021 ttgttgaacg agcagcattt gtatgattgc tgcgacggca cgattcagga gtgtttgaag
    10081 gagggcaagt gttcgctggc cactcgattg gcgaatcagg tttgtcgcat ggcggcagcg
    10141 ggaaaggata agggcacgat cgaacgagcg ctcagccaga gggcgacgag catgatgagc
    10201 gcgcgagccg taccgattga caactcacga gcggaacggg cgggagaggc gaacgcacct
    10261 gtcactcttt cgctctatct ttgcgcgcgt tgtccttatt gcgcgaggct cgttccgcaa
    10321 ttgcacaagg agattacgga aggcgctttg aaaggcaagg tcaaggcata tgtgcgggag
    10381 tttcccatcc gaagccatcc tggctccacg gaaggaggga tggggatggt tgcggcgcgc
    10441 cgcttgggga aaggatggcc tttcttgctc catctatatc gaatttttga tcgcttcgat
    10501 cctgccaaca tgccgaaatg cgctgctgaa tacggcatgg acgaagaaga attccgcaac
    10561 ttgctcaacg atgaaggcgt gcgacgcgag ctgacggctt ccaagaaaga aggcgtgcgc
    10621 aatcgcgtgg atgccacccc cacgctattc atcaatgggc gcaagtacac cggcgacctg
    10681 agcatcgaaa ccgtcgtcga cgtgcttgcg gaagaagcag agcgagttcg cgcaaaagcg
    10741 accaagtaaa gccaaccgtc atatctttcc ttgcagcgga tcatcactcg gtcttgcgtc
    10801 tgccgatgtg tcaggtactc ttctgacatg aacgccaaga tggttttcgc aagcttcggg
    10861 gtcttgttgg ccgcgaacgg gcttggatgt tcgtcgtctg ctgaagctct tttcgatcgc
    10921 aaacccgtac cggggaccga tctcgatgcc tcggctgata cgggagagag cggaggatct
    10981 cagaatgtgg gaggttcggg gggggcttcc acgggaggca gtggaggcgg caactacggc
    11041 ggaactgggg gatctggcgg c
//
LOCUS       DAOSOR010000134        10952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5971_length_10952_cov_4.090759, whole genome shotgun sequence.
ACCESSION   DAOSOR010000134 DAOSOR010000000
VERSION     DAOSOR010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 10952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10952
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5971_length_10952_cov_4.090759"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="PK710_09355"
     CDS             complement(<1..659)
                     /locus_tag="PK710_09355"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOT09966.1"
                     /translation="MRVSRWFAAQLETGSFRGYPGAHVLSKAYEAVASTRLARPVTLP
                     ARVRVVGVGSAVLGGAGKTLVAIAYARRLAQAGMRVSFVGHGYGAHPREARCCEGDET
                     VLEIGDDALLAMRALRPYEVGVWVGKDRNEVLAKAASGVDVIVVDGLLQTRPCELFRS
                     VLVVDGQVPFGSGACPPLGDLRGSVRSLIGHFDEVVVVRDVLSKQVGLPTMGEGVQRR
                     Q"
     gene            complement(662..1387)
                     /locus_tag="PK710_09360"
     CDS             complement(662..1387)
                     /locus_tag="PK710_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOT09967.1"
                     /translation="MSDPRVLVIIPTYNERDNLPILIDAVFSVMDKVHVLVIDDASPD
                     GTGQVADAMARRDPRVCVLHRAAKLGLGTAYVEGFKLALERGFEVVVEMDADMSHDPR
                     YLPALIGAVRDGADVAVGSRNIPGGGVQGWGLGRQLLSKGGSLYSRVILGVDTRDLTT
                     GFKAFSRRALETIGVDEIRSNGYSFQIETTYRALCWGLHVVEVPIIFVDRRVGQSKMS
                     RRVFVEAVGMVWKLRVEALLNKL"
     gene            complement(1401..2177)
                     /locus_tag="PK710_09365"
     CDS             complement(1401..2177)
                     /locus_tag="PK710_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOT09968.1"
                     /translation="MKECTSRTAAAVIIGNELLNGKIEERNVVVLARVLRTLGIALRR
                     IVVVPDEVPTIVQEIRSLSAAFDLVFTSGGVGPTHDDVTVEAVAQAFDTCVQVHEPTA
                     EMIRRHFGDRLTPGHLLMARVPQGCTLVTDEMVQWPTIVMRNVWVLPGVPEIFHKRLG
                     MMKRHLAGAEPFVCRVVHTRLDEGNLVPLLNQIVEQYPDVDVGSYPQWNHPTIMTKLT
                     FDGLDEQAVERACEAFVRLLPEGEPVLVERDCVALSHDVG"
     gene            complement(2187..2576)
                     /locus_tag="PK710_09370"
     CDS             complement(2187..2576)
                     /locus_tag="PK710_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT09969.1"
                     /translation="MSKSILIVDEERHIRTLLEHTLSLFEEEDVTIRLAASGQEALEE
                     CRRSPPDLVFVDARLSDVHGKRFFDSLRLDPGARHTQIILMVDKGWEVDVHHEAGGSI
                     THVIVKPFDPEALRLLTGRLLGIDVEP"
     gene            complement(2573..4078)
                     /locus_tag="PK710_09375"
     CDS             complement(2573..4078)
                     /locus_tag="PK710_09375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT09970.1"
                     /translation="MSLRLSEPSVSRRTRSFIRRWNKWEARRVVESLVVGPGGPVLLA
                     HTDGTELAWVRGKGTWTDLVLRGSKLLGSPAVAQLVGDVESLSRDVVDTIEWMVSVVA
                     EFDQEMEDLSNHALERYRELTLLYDFSERSSTASAIEEVLDLTLRKAVALVHASGASV
                     VRLQGDGASTLASVGTVPDIPLASIEKVVALGRPLVGMLPETLREVEDRNWSSTLACF
                     PLRTAGKTMGVMTVLAGAGRELRPEDQRLLSALASLSATRIEQADLAEANVRKRELAA
                     IGHVTSAIVHDFKNPLTAIRGFAEMIQMDHVQPSEHAALAEQVIVNADRLWAMVEEVL
                     HFVRGNRNALELVPISARELTERLNRVLRHGHPASIELVVDLEALGTVVLDVQKFERV
                     IVNLVRNASEAIIGRGVIEVRGSAVDGSSEYYQIWVRDDGPGISQKIRDTLFDPFVTA
                     NKSGGTGLGLAIVKKIVEEHGGAVTVDSEAGRGACFRIVLPRLQDHSRGES"
     gene            complement(4205..5317)
                     /locus_tag="PK710_09380"
     CDS             complement(4205..5317)
                     /locus_tag="PK710_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09971.1"
                     /translation="MSKDKPVQEQWNYSRYERLLSKLGVRRTVRGWCVRAAENANLPS
                     RAGCCDECGAKAKYSRRAAVSLRMVGRPVPELRVSLCQECFVRTWHRTLWSVASGFFA
                     LAGGIGGAWVVFALWPHSAWVAMVVGSIVGSCLGMLMSWWIPGRVGNQGGWSGVPARI
                     VESVEQGCTIQIASRILADRLMGTGIPVVRCEVVEQDMMPGICGGIGALVMVTTLLWA
                     WWHPVVRVVNVGEQPVVVIVDGRTLAVVPGVPTEVSGAGEKVRIARGWRQLRAMKLDG
                     EVVEEARVFVGHGKEQIYMPNSNGRCFRVEQRAYGRAKRPRPEVIELPSEKHFHTLEV
                     PIDAWFEPNPWSGGNRWLSGGVRRVVRQQRCRPNPP"
     gene            5362..7581
                     /locus_tag="PK710_09385"
     CDS             5362..7581
                     /locus_tag="PK710_09385"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOT09972.1"
                     /translation="MVSSSSASPFAEATPLPTVLNPPQLEAVTHTSGPLLVLAGAGSG
                     KTRVITYRIAYLLAHRVAPYRIYAVTFTNKAAKEMRSRIERLVGSDLASDLWLGTFHA
                     TCARLLRRYHDTAGLRKDFVIYDDSDQRAVMTRVVNDLNLDDRRFPPKLLLSMLHSYK
                     QQGTSLNDIPVASPADQTIANAFAEYQRKLLAANAVDFDDLIIHVMNAAEDRSSIVGE
                     ELRSRFDHLLVDEFQDVNPVQYRLVRALATDHHSVCVVGDDDQSIYRWRGADIRIIRG
                     FKNDFAKSRIVKLEQNYRSSGNVVRTALAVIKPSREREPKELFTTNPPGTPVKVVATR
                     DEKDEGSWVVNAIQNAMAKGTPPNEIAVFYRIHAQSRVIEEALRSHAIPYQIIGGMRF
                     FDRAEVKDVLAYLRVLVNPTSDVDLLRIINVPARQIGATTVNRLVLQATTTDRSVYDV
                     LTSDLHAAGLNRAACKRVAAFGELLKKLRDDVAHLGPRALAEQVLHVTGYESSLLADD
                     TPESLARIENLQELLGSLSEYEDETLQTGTTPSLSGYLERVSLISDIDQMQDAPRISM
                     MTVHGSKGLEFDNVIVTGFEEGLFPYHSATSASDPEEERRLAYVAFTRARRHLTITHT
                     ASRMIFGSYRFNQPSTFLLDLPSDAVVSECSPALAHVRTPSVSTTPTMPTAPGERYVE
                     LDDNESSLAPGRTVMHQRYGTGTILSVDAGQTPAIAFVSFPAFGKKRIVVSHLRPSS"
     gene            complement(7632..10244)
                     /gene="clpB"
                     /locus_tag="PK710_09390"
     CDS             complement(7632..10244)
                     /gene="clpB"
                     /locus_tag="PK710_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOT09973.1"
                     /translation="MRIDRMTTKSQEAVRQAVDAATRRGNPEVTPEHLLVAILNQESG
                     VGGPLIQKAGGDPAKIAKDLMEHVESLPRVTGGADPQLSRRGLHVLQKAEDQAKALRD
                     DYISVEHILLAAVQHDDRIRSVFRGHGLGFDKLLEALASVRGSQRITDRDPEDKFQAL
                     DKYTRDLTADAYQNKLDPVIGRDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAIAEGI
                     AQRIARGDVPESLRNKRLCSLDLGALVAGAKYRGEFEDRLKAVLKEIEAEQGKVVLFI
                     DELHTLVGAGAAEGAMDAANLLKPMLARGQLRCIGATTLDEYRKRIEKDAALERRFQP
                     IVVTQPGVNDTIGILRGLKERYEVHHGIRIQDAALVAAATLSHRYISDRFLPDKAIDL
                     VDEAASKLKMEIDSMPGPIDQVERKLMQLQIEQQALKKERDSASKARLEEVQQAVSDL
                     MDERDAMRAQWLREKELIGQIQDLQAKLETTRVEMERVQRTGDLGRAAEIQYGHIPEI
                     ERQLNSLRQELARVQEKVSYLKEEVTDQDIAAVVSKWTGIPLEKMLESEMHKLLRMEE
                     GLRARVVGQEAALHAVANAVRRSRAGLSDEGRPVGSFLFLGPTGVGKTELGRALAALL
                     FDDERAMIRIDMSEYMEKHSVSRLIGAPPGYVGYDEGGQLTEPVRRRPYSVILFDEIE
                     KAHPDVWNTLLQVLDDGRLTDGQGRTVDFKNTVLILTSNLGTEAAMAVEERADLDDDA
                     RRRQVRDVVLEVVRRSFRPEFLNRIDEVVVFQRLGRKEISAIVDIQLGRLRDRLAKRD
                     LKLEVSDGAKERMGDLGWDPQYGARPLKRAIQKNLEDPISLRLLAGDYVSGDTVVVDA
                     SGGELVLDRTPGKP"
     gene            complement(10431..>10952)
                     /gene="gyrB"
                     /locus_tag="PK710_09395"
     CDS             complement(10431..>10952)
                     /gene="gyrB"
                     /locus_tag="PK710_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506219.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HOT09974.1"
                     /translation="AARARLEKTNPDLFPLEVEVQWEYEHGAACIVFSPRPGSGVARC
                     SVGWQLTESAEYQELWSIHEDLLSIGPAPYVAFGKDDKTDLIDAEALIALLDNRGLKG
                     ITLGRYKGLGEMNAHELWETTMNPDARTLLQVRVDDAVNADEIFNILMGDQVEPRRKF
                     IEENALNVRNLDI"
BASE COUNT         2526 a         3796 c         2366 g         2264 t
ORIGIN      
        1 atctggcgcc tctgtacccc ctcccccatc gtcggtaacc caacctgctt cgataatacg
       61 tcccgaacca cgaccacctc gtcaaaatgc ccgatcaacg atcgtaccga cccgcgaaga
      121 tctcccaaag gcggacatgc ccccgaccca aaaggaacct gcccatcgac caccagcacc
      181 gaacgaaata attcgcacgg cctcgtctga agaagcccat ccaccacaat cacatcaacc
      241 cctgaggctg cttttgccag aacctcgttc cggtcttttc ccacccatac ccccacttca
      301 tacggacgaa gcgcgcgcat cgccaacaac gcatcatccc caatctccag caccgtttca
      361 tccccttcac aacatctcgc ttctcttgga tgcgcaccgt agccgtgtcc cacgaacgat
      421 acgcgcatgc ccgcctgagc gagccgccgc gcataggcaa tggccaccaa ggtttttccc
      481 gctcccccca gcaccgcgct tcccacccca accacccgca ctcgagccgg caacgtcaca
      541 ggccgcgcaa gtcgcgtcga agccaccgct tcataggctt tcgacaatac atgagccccc
      601 gggtacccac gaaacgaacc cgtctccaac tgcgccgcaa accaacgcga aacccgcacg
      661 actacagctt gttgagcaac gcttccacgc gaagcttcca aaccatgcca accgcttcca
      721 cgaacactcg acgactcatc ttcgattgcc cgacacgtcg atccacgaaa atgattggca
      781 cctcgacgac atgcaatccc caacacaagg cacgataggt cgtctcgatt tgaaatgaat
      841 aaccgttgga tcggatctca tcgacaccga tggtttcgag ggcccgacga gaaaacgcct
      901 tgaatccggt ggttaaatcc cgtgtatcga ccccaaggat gacgcgagaa tacagcgacc
      961 cccccttcga tagaagctgt cgtccaagac cccatccttg cacaccacct ccgggaatat
     1021 tgcgagaccc caccgccaca tccgctccgt cgcgcacggc accgatgagc gcgggcaaat
     1081 agcgagggtc gtgagacata tccgcgtcca tttccaccac gacttcgaac ccccgctcga
     1141 gagccagttt gaatccttcg acgtaggccg taccaagacc aagtttcgct gcacgatgca
     1201 acacacagac acgcgggtca cgccttgcca tcgcatcggc aacctgcccg gtcccatcgg
     1261 gcgaagcgtc gtcaatgaca agcacgtgga ccttgtccat cacggaaaat accgcatcga
     1321 tgaggatagg aagattatcc cgctcgttgt acgtgggaat gatcaccaaa acacgcggat
     1381 cggacatggc gttgcttcgc ttacccgaca tcgtggctga gagcaacgca gtctcgttcg
     1441 acaagcaccg gttcgccttc cggcagcaaa cgaacaaaag cttcgcaagc tctttccaca
     1501 gcctgctcgt cgagaccgtc aaacgtgagc ttggtcatga tcgttggatg attccattgc
     1561 ggataggaac caacatccac gtcaggatac tgctcgacaa tctggttgag caacggcaca
     1621 aggtttcctt catccagccg cgtatgaacc acacgacata cgaaaggttc cgccccagcc
     1681 aaatgccttt tcatcatccc gagacgcttg tgaaatattt ccggtacccc cggtaatacc
     1741 cacacattgc gcatcacaat cgtcggccac tggaccattt catcggtcac gagcgtgcaa
     1801 ccctgcggca ccctcgccat cagcaaatgc ccgggtgtca accgatcccc gaaatgacgt
     1861 cgaatcatct ccgccgtcgg ctcatgaacc tgaacacagg tatcaaacgc ttgggcaaca
     1921 gcctccacgg tcacgtcatc gtgggtcggt cccacacccc cactggtaaa cacaagatcg
     1981 aatgccgccg aaagcgatcg aatttcttgc acaatcgttg gcacttcgtc aggcaccacg
     2041 acaattcgac gcaatgctat ccctaacgtg cggagaacac gcgcaaggac cacaacgttc
     2101 cgctcttcga ttttcccatt gagcagttcg ttgccaatga tgaccgcagc cgcggtgcgc
     2161 gaagtacatt ccttcatgat gggtgatcaa ggctcgacat cgatgccgag taacctgcca
     2221 gtaagcaaac gcaacgcttc cgggtcgaaa ggtttgacga tgacgtgagt aatcgatccg
     2281 ccagcttcat ggtgtacatc gacctcccac cccttgtcga ccatgaggat gatctgggta
     2341 tgccgcgcac caggatccaa gcgtagagaa tcgaaaaacc gcttcccatg aacatccgaa
     2401 agtcgagcat cgacaaacac gagatcgggt ggagatcggc gacattcctc caaagcctct
     2461 tgcccacttg ccgcaagcct aatcgtcaca tcctcctctt cgaataagct gagtgtgtgc
     2521 tcgagtagcg ttcgtatgtg gcgctcttcg tccacgataa ggatcgattt gctcatgact
     2581 ccccccttga atggtcttgt agtcgcggca acacaatccg aaaacacgca ccacgaccgg
     2641 cttccgaatc cacggttacg gcacccccat gctcctcgac gatctttttt acgatcgcca
     2701 aacccaaccc cgttcccccc gatttgttcg ccgtcacaaa tggatcgaaa agagtatcgc
     2761 gaatcttctg cgatatccca ggcccatcgt cgcgaaccca tatttgataa tactccgacg
     2821 atccatcgac tgcactaccc cgcacctcga taacacccct gccaataatg gcttcactcg
     2881 cgttgcgaac aagattgaca atgacccgct cgaatttctg aacatcgagc accaccgtcc
     2941 ccaacgcctc caaatccacc acaagctcaa tactcgccgg atgaccatgg cgcaaaacgc
     3001 gattgagcct ctcggtcaac tcccgagccg aaatcggcac taattccaat gcgttacggt
     3061 ttccccgcac gaaatgtaaa acctcctcca ccatcgccca aagccgatcc gcattgacaa
     3121 tcacctgctc cgccaatgcc gcatgctccg aaggctgcac atgatccatc tggatcattt
     3181 ccgcaaaccc acgtatcgcc gtcagcggat ttttgaaatc atggacgatc gcactcgtga
     3241 catgacctat cgccgccaac tctctttttc gtacattcgc ttccgccaaa tccgcttgct
     3301 cgatacgtgt cgccgataga gatgccagcg cagacagcaa ccgctgatcc tccggtcgca
     3361 attcccgtcc cgctcccgcc aataccgtca tcactcccat ggttttgccc gcggtgcgaa
     3421 gcggaaaaca cgccaacgtc gacgaccaat tcctatcctc aacctcccgc aacgtctccg
     3481 gcaacatccc caccaatggc cttcccaacg cgaccacctt ctcaatcgac gccaagggaa
     3541 tatccggtac cgttcccacc gacgccaacg tcgatgcacc atcgccctgc aaacgaacca
     3601 cagaggcccc cgaggcatgc acaagcgcta ccgctttgcg caacgtcaaa tccaatacct
     3661 cctcgatcgc agaggccgtg ctggatcgtt cagaaaaatc ataaagcaat gtcaactcac
     3721 gatatcgctc caaagcatga ttgctcagat cctccatctc ctggtcgaac tcggctacca
     3781 cactcaccat ccactcaatg gtatccacca cgtcgcgcga taatgattcg acatctccca
     3841 caagctgagc caccgccgga gacccaagca gcttcgaccc ccgcaacacc aaatccgtcc
     3901 aggttccctt cccccgaacc cacgcaagct ccgtgccgtc ggtatgcgcc agaagaaccg
     3961 gacccccagg ccccaccaca agagactcca cgactcttcg cgcttcccac ttgttccatc
     4021 gcctgatgaa acttcgggta cgtcgcgata cggacggctc gcttaggcga agagacattg
     4081 tccctacgat atcggttcat cctgcctatt cgcaagatcc tcggcatcca ccccctgtcc
     4141 cccctgcgat ggtcatggct cgttgagaca ctcccatcct gtcgatcaac gacaaacccc
     4201 agtgtcatgg cggattcgga cgacaacgtt gctgtcgcac cacacgccgt actccccccg
     4261 aaagccaacg gtttcctccg gaccaaggat tcggctcgaa ccatgcgtca atcggtactt
     4321 ccaaggtatg gaaatgcttc tccgaaggta actcgatcac ctccggccgt ggtcgcttcg
     4381 ctcgcccata cgcccgctgc tccacacgaa aacaacgacc gttggaattc ggcatatata
     4441 tctgctcttt gccatgcccc acgaaaaccc gagcttcctc tacgacctca ccatcgagtt
     4501 tcatcgcacg aagctgcctc catcctcgag cgatcctcac cttctcaccc gcccccgata
     4561 cctccgtcgg cactcctggc accacggcca aagtcctgcc atcgacgatc accaccacgg
     4621 gttgctcccc cacattcacg actcgaacca ccggatgcca ccatgcccac aatagcgtcg
     4681 tgaccatgac caacgcacca atccctccac agattcccgg catcatatcc tgctccacga
     4741 cctcgcagcg caccaccggg atgcctgtcc ccatcagccg atccgccaaa atccgtgacg
     4801 ctatctgaat ggtacaccct tgctcgacag actccacgat ccgagctggc acgccactcc
     4861 atccaccttg atttcccaca cgccctggaa tccaccagct catcagcatt cccaggcagg
     4921 aacccacgat cgaccccacc accatcgcaa cccaagcaga atgcggccat aacgcaaata
     4981 caacccaagc gcctccaatc ccacctgcca acgcgaaaaa ccccgatgcc actgaccata
     5041 acgtgcgatg ccaggttcgc acgaagcact cttgacacag cgatactcgt agctccggaa
     5101 cgggtcgccc aaccatgcgc aaggacaccg ccgcccttcg actgtatttc gccttcgccc
     5161 cacattcgtc acagcaaccc gcgcgcgaag gcaaattcgc attttccgcc gctcgaacac
     5221 accaccccct caccgttcgt ctcaccccca gcttcgacaa taaccgctcg taacggctgt
     5281 aattccactg ttcttgaacc ggcttatcct tgctcacaac aatccctgac gtcgatcccc
     5341 gaacgtgcta ccgcgagccc catggtttcg tctagctccg cctccccctt tgctgaagcc
     5401 accccacttc ccacggtcct caacccaccc caactcgaag ccgtgaccca tacgtccgga
     5461 cccttgctcg tccttgccgg agccggaagc ggaaaaacac gagtcattac ctaccgtatc
     5521 gcatacttgc tcgcccatcg cgtcgcccct taccgcatct acgccgtcac cttcaccaac
     5581 aaagccgcca aagaaatgcg ctctcgcatc gaacggctgg tcggttccga tctcgcctcg
     5641 gacctgtggc tcggcacgtt ccacgctacc tgcgcacgct tgctccgccg ctaccacgat
     5701 accgccgggc ttcggaaaga cttcgtcatc tacgacgact ccgatcaacg cgccgtgatg
     5761 acccgcgtcg tcaacgacct gaatctcgac gatcgccgct ttcctcccaa actcctccta
     5821 tccatgttgc attcctacaa acagcaagga accagtctca acgatatccc cgtcgcctcc
     5881 cccgccgacc aaacgatcgc caacgctttt gccgaatacc aacgaaaact actcgccgcc
     5941 aacgccgtcg acttcgatga cctcatcatc cacgtcatga acgccgccga agaccgctct
     6001 tcgatcgtcg gcgaagaact ccgaagtcga ttcgaccacc tgctcgtcga tgagttccaa
     6061 gatgtaaatc ctgttcaata tcgcttagtt agagccttgg ccaccgacca ccacagcgtc
     6121 tgtgtcgtgg gagacgacga ccaaagtatt taccgatggc gcggtgcgga tattcgaatc
     6181 atacgaggct tcaaaaacga tttcgcaaaa tcacgaatcg tcaaactcga acagaattat
     6241 cgctcttccg gaaatgtcgt gcgcacggct ctcgccgtca tcaaaccaag ccgcgaacgt
     6301 gaacccaaag aactgttcac gaccaatccc cccggaacac ccgtcaaggt cgtggctacc
     6361 cgcgacgaaa aagacgaggg gtcctgggtt gtgaacgcca tccaaaatgc catggcgaaa
     6421 ggcaccccac ccaacgaaat cgcggtgttc tatcgtattc acgcacagtc ccgcgtgatc
     6481 gaggaagctc ttcgctccca cgctatcccc tatcaaatca tcggcggtat gcgctttttc
     6541 gatcgcgcgg aagtcaaaga tgtcctcgcg tacctacgcg ttttggtcaa tccaaccagc
     6601 gacgtcgacc tgctgcgcat catcaacgtt cccgctcgac agatcggcgc taccaccgtc
     6661 aaccgcctcg tgctgcaggc tacgaccacc gatcgatccg tctacgatgt gctgacgtcc
     6721 gatctgcacg ccgcgggact caatcgggcc gcctgcaaac gcgtggcagc ttttggtgag
     6781 ctgttgaaaa aactccgcga tgatgtagct caccttggac ctcgcgcttt ggccgaacaa
     6841 gtccttcacg tcaccggcta cgaaagctcc ttgctcgccg acgacacccc cgaatctctc
     6901 gcccgtatcg aaaacctgca agagcttctt ggctcgctgt ccgaatacga agacgagact
     6961 ctgcaaacgg gaaccacacc atcgctatcc ggttacctcg aacgcgtttc gctcatctcc
     7021 gacatcgatc agatgcaaga cgctccccgc atatccatga tgaccgttca cggctccaaa
     7081 ggactcgagt tcgacaacgt gatcgtcacg ggcttcgaag aaggcctttt tccttaccat
     7141 tccgcaactt ccgccagcga tccggaagaa gaacgacgac ttgcctacgt ggcgtttacg
     7201 cgtgcccggc gccacctgac catcacacac accgcatccc gtatgatttt cggttcctat
     7261 cgcttcaacc aaccaagtac gtttcttctc gaccttcctt ctgatgccgt tgtctcggag
     7321 tgctcaccgg ctctcgctca cgttcgcact ccttctgtct ccacaacacc aacgatgccc
     7381 acagcgcccg gtgagcgtta tgtcgaactc gatgacaacg aatcgagtct ggcacccggc
     7441 agaaccgtca tgcatcaacg ctatggaacc ggtaccatcc tttccgtcga tgccggacaa
     7501 actcccgcta tcgcattcgt ttcctttcct gctttcggca aaaaacgaat cgtcgtctcc
     7561 cacctgcgac cttcgtcata acgccttttc cctacctccg ccgatcattc caaacccaca
     7621 tttctcaact gtcatggctt gcccggcgtc cgatcaagca ccaattcccc tcccgacgca
     7681 tctaccacga ccgtatctcc cgacacgtaa tcacctgcga gcaaacggag cgatatgggg
     7741 tcctccaagt ttttttgaat ggcacgcttg agcggacgcg ccccatattg cgggtcccag
     7801 ccaagatcac ccatgcgctc tttcgcccca tcggaaacct cgagcttcag atctcgcttc
     7861 gccaaacgat cccgcaaacg accgagctga atgtccacga tggccgaaat ctcctttcgt
     7921 ccaagacgct gaaacaccac cacctcgtcg atacgattca gaaactcagg acgaaacgag
     7981 cgacgaacga cctccaacac aacgtctcgc acttgcctgc gacgagcatc gtcatccaaa
     8041 tccgcgcgtt cctctacggc cattgctgct tccgttccga gattcgatgt gagaatcagc
     8101 acggtgtttt tgaaatcaac cgttctaccc tgaccatcgg tcaatctccc atcgtcgagc
     8161 acctgcaaca atgtgttcca cacatccgga tgagccttct cgatctcgtc gaaaagaatc
     8221 acgctatacg gacgcctacg cactggttcc gtgagctgac ctccctcgtc atacccaacg
     8281 tatcccggag gtgcgccaat gagacgagat acggaatgct tttccatgta ctcgctcata
     8341 tcgattcgaa tcatcgctcg ttcgtcatcg aacaacagag ccgccaaagc acgccccagt
     8401 tctgtttttc caactcccgt gggcccaaga aataaaaacg acccgaccgg acgaccttcg
     8461 tcgctgagcc ccgcgcggga ccgacgtaca gcattggcta ccgcgtgcaa tgcggcctcc
     8521 tgtccaacca ccctggcgcg taacccttct tccatacgca agagcttgtg catttcgctt
     8581 tccaacattt tctccaaagg aatccctgtc cacttggaga caacggccgc gatgtcctgg
     8641 tccgtgacct cctctttgag gtaactcacc ttttcctgaa cacgcgcaag ctcctgacgt
     8701 aaggagttta gttgacgctc gatctcggga atgtggccgt attgaatctc agccgctctt
     8761 cccaaatccc ccgtgcgttg cacgcgttcc atctcgactc gcgtggtttc cagcttcgct
     8821 tgcagatctt gaatctgacc aatgagttcc ttttcccgaa gccattgcgc acgcatcgcg
     8881 tcccgctcat ccatgaggtc cgagaccgcc tgctgcactt cctccaaacg cgctttcgag
     8941 gcggaatcac gctctttttt caaagcttgc tgctcgatct gaagctgcat gagctttctt
     9001 tcgacctgat cgatcgggcc tggcatcgaa tcaatttcca tcttcaactt cgaagccgct
     9061 tcatcgacca gatcgatggc tttatccggc aaaaaccgat ccgatatata ccggtgcgac
     9121 agggtggccg cggcaaccaa cgccgcgtcc tggatgcgaa tgccgtgatg cacctcatat
     9181 cgctctttca aaccccgaag aattcctatc gtgtcgttga ctcccggctg agtcaccaca
     9241 atcggttgaa accgacgctc caaggcagcg tctttttcga tgcgttttcg gtattcatcc
     9301 agggtggtgg ctccaatgca gcgaagctgc cctcgcgcta gcattggctt caataagttc
     9361 gcggcatcca ttgcgccttc ggctgcaccc gcgcccacca aggtatgaag ctcatcaatg
     9421 aacagaacga cttttccctg ctccgcttca atctccttga gtacggcttt gagacgatct
     9481 tcgaactcac ctcgatactt cgcccccgcc acgagagcac ccagatccaa agaacatagt
     9541 cgcttgtttc gaagggattc cggcacgtcc ccgcgggcga tgcgttgcgc aatcccctcc
     9601 gcaatcgctg tttttccaac gccaggctct ccgatcaaca ccggattgtt cttcgttcgc
     9661 ctggataata cctgcatgac tcgccgtatc tcctcgtcac gaccaatcac cggatccagc
     9721 ttgttctgat aggcgtccgc cgtaagatcc ctcgtgtact tatcaagggc ctggaacttg
     9781 tcttccggat ctcgatccgt aattcgttgc gaaccacgta cgctagccaa cgcctccagc
     9841 aatttgtcga accccaaacc atgcccgcga aacaccgacc gaattcgatc gtcatgctgc
     9901 acggccgcca acaaaatatg ctccaccgaa atgtaatcgt ctcgaagcgc cttcgcttga
     9961 tcctctgctt tttgaagtac gtgtaggcca cgtcgagaaa gctgtggatc ggcgcccccg
    10021 gtgacgcgag gaagcgattc cacgtgttcc ataaggtctt tagcgatttt ggcagggtcg
    10081 ccgcccgctt tttgaatcag cggaccaccc acaccgcttt cctgattcaa tatcgctacc
    10141 aacagatgtt ccggcgtaac ctccggattg ccacgacgtg ttgctgcatc cacggcctgt
    10201 cgtacggctt cctggctctt ggtagtcatc cgatcgatac gcataccggc tccttgacgc
    10261 cacccatcct cgctgtcttt gtccgcgaag ggttgcgtga aaatgaatcg tctggtcggg
    10321 aaatcaaacc gggttggttg aagcacccga tgttttcaga agataagtcg agaaatgccg
    10381 ggatcaagac ccggcatgag tcctgcagtc aaatgggcac ttccgcttat tcaaatgtcc
    10441 agattgcgta cgttgagcgc gttttcttca atgaacttcc tgcgcggctc aacttgatcc
    10501 cccatcagaa tgttgaagat ctcatccgcg ttgaccgcat catcgacccg aacctgcaag
    10561 agcgttctgg catcgggatt catcgtcgtc tcccacaact catgggcgtt catctccccc
    10621 aaacccttgt accgccccaa cgtgatgccc ttcaaacctc ggttgtccaa caacgcaatg
    10681 agagcctccg catcgatgag gtcagtcttg tcgtctttgc caaacgccac atacggtgca
    10741 ggtccaatgg acagcaagtc ctcatgaatg gaccacaact cctggtattc ggcgctctcg
    10801 gtgagttgcc aacccaccga acaacgagcc actccagaac caggtctcgg tgaaaatacg
    10861 atgcacgccg caccatgttc atattcccac tggacttcga cctccaacgg gaaaaggtcc
    10921 ggattggtct tctcgagccg cgcacgagcc gc
//
LOCUS       DAOSOR010000135        10917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_5997_length_10917_cov_4.409225, whole genome shotgun sequence.
ACCESSION   DAOSOR010000135 DAOSOR010000000
VERSION     DAOSOR010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 10917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10917
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5997_length_10917_cov_4.409225"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="PK710_09400"
     CDS             <1..1113
                     /locus_tag="PK710_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09975.1"
                     /translation="ELSEHQRLERAKRADLRIKEIREDLMSRLTPEEAEAKRRQMPNP
                     GLMNETAVEIEMRKQMRERVGARIAGLGEDFRNTARESIERMLIEMFVKASYEGGRLE
                     VLLPAGKTLVDRIDGLGRSGHVSESVVRYQNMEMAKADVAFEVLSRYFARQIIDILDA
                     TDAGDREIREKEMQGLEQFFGMSIADRAAQRGTGVLNQVRARVGLNKGEEEEEPQPAL
                     GSGGFPSAGAAYFPSSPTRVKRKGENEEESAAERGDDKGYDYPEHKRDWSKMLGRVRT
                     DIDRICNFLEALAKHPRGLQKYHEEAVRTVHDSWIDREGVQSLRRWVRSECARIWPHK
                     FAAIEAEKARARADIDALEELFGSNEPVDNENVAAS"
     gene            1134..3104
                     /locus_tag="PK710_09405"
     CDS             1134..3104
                     /locus_tag="PK710_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09976.1"
                     /translation="MAYDLTPLRSYVRFYQSVVTQITAAWEALLRTHEAARSVLAPFA
                     QPFSLVRHQALLATLADPPALPPALGSLWLPQDEKDLSGLTDVYEVVGRFTFQGEDDE
                     NLARIQAVVSSARNEIHLQRTRIAELLKLPEVARAQATRLELEEAKSASERKAAKLAA
                     FAPLTAALHQRARQTMDAVRGVPIPALSDVKAASDEYRAYAKKLDQVYQTCLPFLKKS
                     IEDMFEFAECEVPSSWPDHLPIQPELPEEFLMVPPSDSPELKRARSYVEALNEEQREL
                     IAARDDIAAMLSRLDGDMNTFRQKEAEAIEEIEKAGWLVSFANKLEEIDKIRANAASF
                     EQQKADRTQRIGDLSQQVKQIETTIRALEQEMATRKQELADANTQLAVERDAEPAFIG
                     KDAWRSRVSDQEQHIEDLRNAFAQREAVLNQMRIDLASIGVRIQTEQSQSSLVDRWLA
                     DARARERSLQAEAAELDKRLGAARPIHTPSVVEAQQLLAEFQNARMEILERIERIKTD
                     ARRNKEENDRIVARLKQIDEERKKMDGFVQSAQVAATQGFEEAMRQLAARRRAAVVHH
                     VEEVLGELEKSLNSVEVVFVEPARAAMLKAHEPTESIAAMVRDHAAKVEPVVQDLFEQ
                     IEPELLQQDAMMSQVQREFCDVAPEACRNAWA"
     gene            3185..4726
                     /locus_tag="PK710_09410"
     CDS             3185..4726
                     /locus_tag="PK710_09410"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HOT09977.1"
                     /translation="MSPLSRYSSIRMLSCVLAALPIACSSSDDASGSPTTDAGKDVST
                     DHAEAGSQDADPHDDSSIGDAPDSDGNTKDSTSDQEASDGDISTDAPADTLHKSYEID
                     TNTAFLNPERGFHGNIELTQPKANYEYVRQKGYTLARAYVRLDDYRNAPIPSAYLASL
                     RNGLSKLREAGIKVVPRFAYNFGMEPDAPLNVVLEHIDQLTPILEEYSDVIAVLHGGF
                     IGAWGEWHSSTHGLDSPANMKTIAEALLEALPDTRMIQVRTPGYRRHIVGSPSGQVDM
                     FGTTSDARIGFLNDCFLSSASDAGTYLDDQTKDRSEAKDFSRYTTTGGETCSIDFGTS
                     RQKCPTALEELADYHWDYLNSDYYGDVLNGWKSEGCYEEVARRLGYRIALESASVSSK
                     AKLGSVLTIALKTRNDGFGKVYNPRPIDVVLRDSAGEAAIVRAVADSRTMLPLAGQSR
                     EITLSVTVPSGLASGKYRLLLSLPDASTQLENDVRYSIRLANVDMWEEATGFNDLGLT
                     VEMEP"
     gene            4958..5125
                     /locus_tag="PK710_09415"
     CDS             4958..5125
                     /locus_tag="PK710_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09978.1"
                     /translation="MGLDGGENGFSQRDWASKLVQSSEETEQERLPGGDLLQAYAPRQ
                     CILGHHGTALN"
     gene            5356..7356
                     /locus_tag="PK710_09420"
     CDS             5356..7356
                     /locus_tag="PK710_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09979.1"
                     /translation="MVYNSRVVMDDDWTSGEPDLDREARQVLDEGASQMQAQAWDDAE
                     RSFRRALAIAVDRDPTIRLEALAGLAEAHIAKAQLEEALICLEQALHISPHHEAVLRR
                     SLDLSVQMKRHGLSLELRRRLLLLPMAEEEQVAQRRAMVHDAIFIVIDTLESFGDYRA
                     EGFDSLDRLRAAQEAAGNTSKATDVMVAQAERQREPHARAMAMVQAADFCAARTGSTA
                     RAVALYEAAIADDPAVQGAFDAIERVLVQRADYRELEKAYHRQAERLEQAGAKPELAT
                     LLGKLAEVRHDRLEDRKGAIDALARRVQLEPSNVEARSALATLLEHDGNVERACRMLE
                     TAAWHAPTRMDTFHALFRMYTKAKKHDQAFQACAALVYLGDADLDEQMVYQQYRPEAG
                     LRPTAALEESDWAELYPLEHDANVRTLLEVIGPAAISHRVALQQASGKLPVLSAKTRQ
                     DPESSTVSAVRSLRWGSQVLGIALPELHIVPDLAAGISAVASQRPAIAIGKAVLSGRS
                     IAELAFLVGRDLTYFRPEHHILLYYPSMPEFTALVTTAIRMALPNRAGTASLRDRGLL
                     EALEAGIDAAGWDKVRAAVQRVESSGSTIDLRSYVRSLEIAATRVGLLLSGDLPTAGQ
                     LLSTDIREVAGLRAADRMRDLLPYAVSAPYASLRAKLGVDAR"
     gene            7481..9148
                     /locus_tag="PK710_09425"
     CDS             7481..9148
                     /locus_tag="PK710_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT09980.1"
                     /translation="MRPTTIASLVASSLFGLLVGMATPCTAHAADPSSRYYAQDTEGI
                     FWFLHISDLHIDPLNNSARKHLTFALGEAVDVIQPIAVFASGDLVDGSVFLPTSGQDK
                     AEWDLYATLLSDANVTPEFFFDMPGNHDTYGDQGLKHYLERSLQGSTTGSLFSDTVHT
                     TSLGDYYFVALNSSGTYGTPFTFGNPQFTNVPELEAGLETHASAQLVFAFAHHHLVPH
                     GATDAQTALGIGGKDDPPGNVSEVMALLEDAGAFYLHGHVHQYKESLQGKLVTVQLAN
                     LAGSPNVDRSSLDAWDKTKFESNIGVGIIDHNAFIYRVTDTTHPWPFVAITAPVDINL
                     QGGGIPAGSTAGVSYEGDFVPYGEQANPYAYDVCLARKDNPVRAVVLSKDPVSSVRLM
                     LDTTEVGPMTAVTSQPGLYAAQMDTTGLEPGVHSLTVLATTASETRGDSIQVNFVKGP
                     CDDLPDAGVDAPDSEPDASDSDSDITSDAQPDAGSTSDATQDSVADADTTDAADTTPP
                     PGDTDDDGGCSCRVGANTSNPWTALALSLIALAFRRRRTTRSPLSSR"
     gene            complement(9165..10172)
                     /locus_tag="PK710_09430"
     CDS             complement(9165..10172)
                     /locus_tag="PK710_09430"
                     /inference="COORDINATES: protein motif:HMM:NF014042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HOT09981.1"
                     /translation="MPTIPPPKIVTMGGGHGQAALLAALRLLPCSITAIVSVADDGGC
                     SGKLREEVGMPPPGDLRRCLSTLARNRNLADAFELRLLDAHQEARSVGNLALFEAYQQ
                     YGSLQKAVDWAAELLQCAGRVVPVAESPGVLVVYDLRDGRIEGETHAQDVSSPMVAMV
                     HGPTKTNPVAVSAIQQADFILLGPGSFFTSILATLTTADVAEAVAKAKAKKVLIANLV
                     EENDPAKSFEVIEYIRLLRDHLTIASIGEEVTFSVLRHHDGDDELGSLPDGTPIMSSR
                     VCYPGQAVHDPILLSRALSRYLGFPAEKAIPSVPPPPNAEDERKLQEHVEAAKRKLRT
                     M"
     gene            10334..>10917
                     /locus_tag="PK710_09435"
     CDS             10334..>10917
                     /locus_tag="PK710_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09982.1"
                     /translation="MLRWPILLGVLLSTTFAAAQSTDTRSELERVRAAADEFDAGRRA
                     FTAEDYSTAAEHFEKADRDAPAPEALRMAIRARLAANQPDRVATLAAAALSRYPNDEA
                     TVALAKEMLEKMPKRLHRITVQCNEPCSLMVDRRLVPGVAATESTVYVEPGQYEITAG
                     WSAGRARSERIDALPENASTMVFQAPPLTTADPEP"
BASE COUNT         2473 a         3037 c         3177 g         2230 t
ORIGIN      
        1 gaactcagcg agcatcagcg tctcgagcgt gccaaacgcg ccgatttacg catcaaggaa
       61 atccgcgaag acctgatgtc tcgactcacg cccgaagagg ccgaagccaa gcgtcggcaa
      121 atgccaaacc cgggtttgat gaacgagacg gcggtcgaga ttgagatgag aaagcagatg
      181 cgtgaacgcg tgggtgcgcg catcgccggg ttgggggaag attttcgcaa tacggcccgt
      241 gagagcatcg agcgcatgtt gatcgagatg ttcgtcaaag catcgtatga aggagggcgt
      301 ctcgaagtcc ttcttccggc tggaaagact ctcgtggacc ggattgatgg gctcggtcgt
      361 tccggacacg tgtccgagag cgtcgtgcgt taccaaaaca tggagatggc caaggccgat
      421 gtagcgttcg aagtgctttc ccgatacttt gcgcgacaaa tcatcgatat tttggacgcg
      481 acggatgccg gcgatcgtga gattcgcgaa aaggagatgc aggggctcga gcagtttttc
      541 ggaatgagca ttgccgaccg agccgcccaa cggggcacgg gagtgttgaa tcaggttcgc
      601 gcgcgggtgg gattgaacaa aggggaagaa gaagaagagc cgcaaccagc cctgggcagt
      661 ggaggctttc cttcggcggg tgcggcgtac ttcccttcct cgccgacacg agtcaaacgc
      721 aaaggggaaa acgaagagga atctgccgcc gaaaggggcg atgacaaagg ctacgattat
      781 cccgaacaca aacgagactg gagcaaaatg ctcggccggg ttcgcacgga tatcgaccgc
      841 atctgcaact tcctcgaagc gttagccaaa catccgagag gcttacaaaa atatcatgag
      901 gaagccgtcc gaacggttca cgattcctgg atcgatcggg aaggtgtcca gtcgttgcgt
      961 cgttgggttc gttcggaatg tgcccggatt tggccgcaca aatttgccgc catcgaagct
     1021 gaaaaagcca gagctcgcgc ggatatcgat gctctcgagg aactttttgg aagcaatgaa
     1081 cccgtcgaca acgaaaacgt cgcggcttcc tgatgcgatg aggagaaacg atcatggcgt
     1141 atgacctcac ccccttgcgc agctacgttc gtttctatca atccgtggtg acgcagatca
     1201 ccgctgcctg ggaggcactg ctgcgtacgc acgaggccgc acgaagcgtt cttgccccgt
     1261 tcgcacagcc tttttctctc gtgcgacatc aggcgctcct cgccacgttg gccgacccgc
     1321 ccgcgttgcc acccgcgttg ggttcccttt ggctcccgca agatgaaaaa gatctttctg
     1381 ggctcaccga cgtttatgaa gtcgttggac ggttcacgtt tcaaggcgag gatgacgaga
     1441 acctggcaag gatacaagcg gtggtttcga gcgctcgcaa tgagattcac cttcaacgca
     1501 cgcggatcgc cgagcttttg aaactgcccg aggtggctcg ggcccaggct acccgactcg
     1561 aattggaaga ggccaaaagc gcgtccgagc gaaaagccgc aaaactcgca gcattcgctc
     1621 ctttgaccgc cgccttacat cagcgcgcgc gacaaacgat ggacgcagtt cgcggtgtgc
     1681 cgatccccgc gctctccgat gtcaaagctg cctccgatga gtatcgagcc tatgcgaaaa
     1741 aactcgatca ggtctaccag acctgtttgc cgttcttgaa aaaatccatc gaagatatgt
     1801 tcgagttcgc cgaatgcgaa gtcccctctt cttggccgga tcatctcccc atccaacccg
     1861 agttgccgga agaattcctc atggtgccgc ccagtgactc tccggaactg aaacgagcac
     1921 gcagctacgt cgaagcattg aatgaagaac aacgggaact catcgccgct cgcgacgaca
     1981 tagctgccat gctgtcgcgc ctcgacggcg acatgaatac gtttcgacaa aaggaggccg
     2041 aagcgatcga agagatcgaa aaggccggat ggctcgtcag tttcgccaat aaactcgagg
     2101 aaattgacaa aatacgggcc aacgccgcca gctttgaaca acaaaaagcc gatcgtacgc
     2161 agcgaattgg tgacctcagc cagcaagtca aacaaatcga aaccaccatt cgtgctctcg
     2221 aacaagagat ggctacacga aaacaagaac ttgcagacgc caatacgcag cttgccgtcg
     2281 aacgcgatgc ggaaccggcg ttcatcggaa aagatgcctg gagaagccgc gtgtcggacc
     2341 aggagcagca tatcgaggac ttgcgaaatg cctttgcaca gcgcgaagcc gttttgaacc
     2401 aaatgcgaat cgatttggcc tccatcggtg tgcgaattca aacggagcaa tcgcaaagct
     2461 ccctcgtcga ccggtggctc gctgatgcac gagcccgaga acgttctctg caagcggaag
     2521 cggcggaact cgacaaacgg ctgggtgccg caaggcccat acatacccct tccgtcgtgg
     2581 aagcccagca actgctcgct gagttccaaa acgcacgcat ggaaatcctc gagcgaatcg
     2641 aacgcatcaa aacggatgcc cgccgcaaca aagaagaaaa tgaccgcatc gtggcacggc
     2701 tcaaacaaat cgacgaagaa cgaaaaaaga tggacggctt cgtccagagc gcgcaagtgg
     2761 ccgccacgca aggattcgag gaagcgatgc gacagcttgc ggcgcgacga cgagccgcgg
     2821 tggttcatca tgtggaagaa gtgctcggag agcttgaaaa atccctcaat tccgtcgaag
     2881 tcgtgtttgt cgaaccagcc cgcgctgcca tgctcaaggc tcatgaaccc accgagtcga
     2941 tcgccgcgat ggttcgcgac catgcggcaa aagtagaacc tgtcgttcag gatttgttcg
     3001 aacagatcga gcccgagcta ttgcagcagg acgccatgat gagtcaggta cagcgtgagt
     3061 tttgcgatgt cgcccccgag gcatgtcgca acgcgtgggc gtgatcgttt gcgcgaacac
     3121 caaacagcgc cagtgatatc attgactcac agtcctccga acatcagcca acccaatgaa
     3181 catcatgagc cccctgtcac gatattcgtc catccgtatg ctgtcgtgcg tcctggcggc
     3241 cttgcccatc gcctgttctt cttcggacga tgcctccgga tcgccaacca ccgacgctgg
     3301 caaggatgtt tccacggatc acgccgaggc tgggtcccaa gacgcggacc cacacgacga
     3361 tagcagcatc ggagatgctc ccgactccga tggcaatacg aaagactcaa catcggacca
     3421 agaagccagc gacggcgata tttccacgga cgctccagcg gacacccttc acaagagcta
     3481 cgaaatcgat acgaatacgg cgtttttgaa tcctgagcgc ggatttcacg gaaacatcga
     3541 gcttacgcag ccaaaagcca attatgaata tgtccgacag aaggggtata cgctcgctcg
     3601 tgcctatgtt cggttggatg attaccgaaa tgcaccgatc ccttcggcgt atttggcgag
     3661 ccttcgaaac ggtttgtcca agcttcggga agcgggaatc aaagtcgttc cacgctttgc
     3721 ctacaatttt ggcatggagc ccgatgctcc gctcaatgtc gttctggagc atatcgatca
     3781 acttacgcca atcctggaag agtattcgga tgtcattgcg gtgctccacg gtgggttcat
     3841 tggcgcgtgg ggcgagtggc attcgagcac gcatgggttg gattctcccg ccaatatgaa
     3901 aaccattgcc gaggcgcttc tcgaagctct gcccgacaca cgcatgattc aagtccgcac
     3961 accaggctat cgacgtcata tcgtgggctc gccctctggc caggtcgaca tgttcggcac
     4021 gacgagcgac gcgcgcatcg gttttctcaa cgattgtttt ctatcctcgg ccagtgatgc
     4081 cggaacgtat cttgacgatc aaaccaagga tcgcagcgag gctaaggatt tttcacgcta
     4141 cacaaccacg gggggagaga cctgttcgat cgactttggc acatcgagac agaagtgtcc
     4201 gacggctctc gaagaacttg ccgactatca ttgggattac ttgaattcgg attattacgg
     4261 agacgtgttg aacggttgga aatccgaagg atgttacgaa gaggtcgctc gaaggcttgg
     4321 ataccgcatt gcgctcgaat ccgcttcggt atcctcgaaa gccaagcttg gtagtgttct
     4381 caccatcgcg ttgaaaacgc gcaatgatgg ttttggaaaa gtctataatc ctcgacccat
     4441 cgatgtcgtc cttcgcgatt cggcggggga ggccgcgatc gtgcgcgcgg tggccgatag
     4501 ccgaacgatg cttcctctcg cgggccaatc ccgtgagatc acgctcagcg tcaccgtgcc
     4561 ctcaggcctt gcctcaggca aatacagact attgctgagt ttgccggatg cttcgacaca
     4621 gcttgaaaac gacgtgcgct acagcattcg actcgcgaac gtcgacatgt gggaagaggc
     4681 gacgggtttc aacgatcttg gattgacagt cgagatggag ccttgaaacg cgagatacag
     4741 ccggcagtcc cacaagacat ttcgtgagag gtgaagcggg gagtacagcc gtcgtttcga
     4801 tggtgggtta ggtttcacga caactcggtt atttcaggaa ccggccctat ctatgcctcg
     4861 cagagaagaa gaccggactt gcagcgtcgg acgatgaatc gatgaggcat ggctcgaggg
     4921 catattgagt gccggaacct gccagtggaa caaaagcatg ggactggatg gtggggaaaa
     4981 cggattttcg cagagggatt gggcaagcaa gctggtgcag tcctcggagg aaacggagca
     5041 agaacgattg cctggcggag atttgctgca ggcttacgcg ccacggcaat gcatccttgg
     5101 ccatcatggg accgcattga attagcagaa gcgttcgcta tcggaagcga caagggggag
     5161 agcggcattg tgacggcaca gaacaataat tatcaataat ttcaataaga taaaagacga
     5221 tgggcgaatg caattccggc tgtccctcag catctaccga ggtagggaaa cgatgaggtg
     5281 tcatggccga ccgcgtcggg acaggcatct gaaaaaataa tggagcggta caacaagcgc
     5341 cagagtcgat ggcagatggt gtacaactcg cgtgtggtca tggatgacga ctggacatca
     5401 ggggaaccgg atttggaccg cgaggcgcgg caagttctgg atgaaggtgc ttcacagatg
     5461 caggcgcaag cgtgggatga cgcggagcgc agctttcgac gagcgttggc aatcgccgtc
     5521 gatcgggatc caacgatacg actcgaagcg ttggccgggt tggcggaagc gcatattgcc
     5581 aaagcgcaac tcgaggaagc cctcatctgt ctcgaacagg cgttgcatat atcgcctcat
     5641 cacgaagcgg tattgcggcg ttcgctggac ctttcggtgc agatgaagcg tcatggtcta
     5701 tcgctcgagt tgcgacgtcg tttgctattg ttgccaatgg ccgaggaaga acaggtggcg
     5761 cagcgaagag cgatggtaca cgacgcgatt ttcatcgtca tcgacacgct cgagagtttt
     5821 ggggattatc gggcggaggg gttcgattcg ctcgatcgtc ttcgagcggc gcaggaagcg
     5881 gcgggcaata cttccaaagc aaccgatgtg atggttgcgc aagcggagcg gcaacgggag
     5941 cctcacgcac gggcgatggc gatggtgcaa gcggcggatt tttgcgctgc gcgaacggga
     6001 agcacggcgc gggcggtagc gctttatgag gcggcgattg ccgatgatcc tgctgttcaa
     6061 ggcgcgttcg acgccatcga gcgggtgttg gtgcagcgag cggattatcg ggaattggaa
     6121 aaggcgtatc atcgtcaagc cgagcggctc gaacaagcgg gagcgaagcc ggaacttgcc
     6181 acgctgctgg gaaagttggc ggaggttcga cacgacaggc tcgaggatcg caaaggcgcg
     6241 attgacgctt tggcaaggag ggttcaactc gagccatcga acgtggaggc gcgttcggct
     6301 cttgcgacct tgctcgagca tgatggcaac gtggagcggg catgccggat gttggaaacg
     6361 gcggcgtggc atgctccgac tcggatggac acctttcatg cgttgtttcg catgtacaca
     6421 aaagcaaaaa aacacgatca ggcttttcaa gcctgcgccg cgctcgtgta ccttggtgat
     6481 gcggatctcg atgagcagat ggtgtatcag caataccgcc cggaggccgg gctcagacca
     6541 acggctgcct tggaagaatc cgattgggct gaattgtatc ccctcgagca cgatgccaac
     6601 gtgcgaacgt tgctcgaggt gattggaccg gcagcgattt cgcatcgggt tgcattgcaa
     6661 caagcatcgg gtaaattgcc ggtgctctcg gccaagacca ggcaagatcc cgaaagctcc
     6721 acggtgagcg cggttcgaag tttgcgctgg gggtctcagg tattgggcat tgccttacct
     6781 gaattgcata tcgtgccgga tttggcagcc ggaatttccg cggtagcttc ccaacggccg
     6841 gccattgcga ttggcaaagc ggtcctatca ggacgttcca tcgcggaact cgcgtttttg
     6901 gtgggcagag acttgacgta tttccgaccg gagcatcaca tcctgcttta ttacccgtcc
     6961 atgccggagt tcacggcgct cgtgaccacg gcgattcgca tggcgcttcc caatcgtgcg
     7021 gggacggcat ccctccgtga ccgagggctt ttggaagcgc tggaggcagg catcgacgcc
     7081 gcgggctggg acaaggtgcg agctgccgtt cagcgggtgg aatcgagtgg cagtaccatc
     7141 gacctgcggt cttatgttcg ttctttggaa attgccgcaa cccgtgtggg cttgctcttg
     7201 agcggcgact tgcccacggc ggggcaactg ctttccactg atatccgaga agtggctggc
     7261 ttgcgggccg cggatcgcat gcgtgatttg ctgccttatg cggtgtccgc gccctatgcg
     7321 tctttacgag caaaactcgg ggtcgatgcg cgataactcc gcttaccgaa cggttgaata
     7381 actcgctccc ccacgttcgc ctccaagctc gaggctttgc tgaaaaacct gccacgcgct
     7441 atagtaggta tcttcacccc cttgttttca taggttcaac atgcgtccga cgaccattgc
     7501 ttccttggtg gcaagttcgc tgtttggctt gcttgtcggg atggcaaccc cttgcaccgc
     7561 ccacgccgca gacccttcgt ctcgttatta tgcccaggac accgaaggaa tattttggtt
     7621 tttgcacatc tcggatctgc acatcgatcc gctgaacaat tcggccagaa agcatctgac
     7681 attcgccctc ggcgaagcgg tcgatgtcat ccaacccatc gcggtattcg cctccggtga
     7741 cttggtcgat ggctccgtct ttctgccaac atcaggccaa gacaaggcgg agtgggacct
     7801 atatgccacg ctgttatccg acgccaacgt cacacccgag tttttcttcg acatgcccgg
     7861 caatcacgat acctatgggg atcaaggatt gaagcattac ctcgaacggt ctttgcaagg
     7921 gtcaaccacc ggcagccttt tctccgacac cgtgcatacg acctcccttg gggactatta
     7981 tttcgtggcg ctcaatagtt caggaaccta cggcacgccg tttacgttcg gcaatccaca
     8041 gttcaccaat gtcccggagt tggaagccgg actggaaaca cacgcttccg cgcagctcgt
     8101 ctttgctttt gcgcaccacc atctcgtgcc gcacggtgcc accgacgccc aaacggctct
     8161 tggcattgga ggcaaggatg acccgccggg caacgtttcg gaagtgatgg cccttctcga
     8221 ggatgccggc gctttctatc ttcatggcca cgtgcatcaa tacaaggaaa gccttcaggg
     8281 aaagctcgtc acggtccagc tagccaactt ggcagggtcg cccaacgtgg accggagttc
     8341 tctcgatgcc tgggacaaga ccaagttcga aagcaatatc ggcgttggca ttatcgacca
     8401 caacgccttc atttatcgcg tcacggacac cacccatcct tggcctttcg ttgccatcac
     8461 cgctccggtg gatatcaatc ttcagggcgg tggaattccg gcgggctcga ccgcgggtgt
     8521 ttcgtatgaa ggggatttcg ttccttatgg ggagcaggcc aatccttacg cttacgacgt
     8581 ttgcctcgcg agaaaagaca acccggtgcg ggcggtggtg ctatccaaag accccgtttc
     8641 gtcggtccgt ttgatgctcg acacgaccga agtcggtccc atgaccgcgg tgaccagtca
     8701 acccggtcta tacgctgccc aaatggacac caccggcctc gaaccgggcg ttcattcgct
     8761 cacggttttg gcaaccacgg cctcggagac tcgcggcgat tcgatccaag tcaacttcgt
     8821 caaaggtcct tgcgatgact tgccggacgc gggagtggac gccccggact cggagccaga
     8881 cgcttccgat tcggactcgg atatcacctc agacgcacaa cccgatgcgg gaagcacttc
     8941 cgatgcgaca caggattccg tcgctgacgc agacaccacc gatgccgcgg ataccacgcc
     9001 tccaccaggg gataccgatg atgatggcgg ctgttcgtgt cgcgttggag ccaacacatc
     9061 aaacccatgg acggctcttg ctctgtccct gatcgcgctt gcctttcggc gtcgtcgcac
     9121 gacaaggtca cctctgtcct cgaggtaggc acgctcggag ccgttcacat cgtacgcaac
     9181 tttcgcttcg ctgcttccac gtgctcttga agctttcttt catcctccgc gttgggcggc
     9241 ggaggaacgg aaggaatcgc cttttcagcc ggaaaaccca aatagcgaga cagggccctg
     9301 gacaacagga ttgggtcatg gacagcctgc ccgggatagc aaacgcgcga ggacatgatt
     9361 ggggttccat caggtaagga cccaagctca tcatcgccat cgtgatgcct gagaacggag
     9421 aacgtcacct cctcgcctat gctggcgatg gtcaggtgat cgcgtaaaag acgaatatat
     9481 tcaattactt caaagctttt tgcaggatcg ttttcctcga cgagattggc gatgagcact
     9541 tttttggctt ttgctttcgc cacggcttcc gccacatccg cggtcgttag cgtggcgagg
     9601 atgctggtga agaaactgcc ggggcccaac aagatgaagt cggcttgttg aatcgctgaa
     9661 acggcgacag gattggtttt cgtaggccca tgcaccatgg cgaccatggg agaagacaca
     9721 tcttgtgcat gcgtttcgcc ctcgatcctg ccatcgcgaa ggtcatacac gacgagtacg
     9781 cctggggatt cggcgactgg cacgacacgt ccggcgcact gtaggagttc cgcggcccaa
     9841 tccaccgcct tttgcagaga accatactgc tgataggctt cgaataacgc caaattaccc
     9901 acgcttcgcg cctcttgatg cgcatcgagc aggcgcagtt cgaaagcatc cgcaaggttt
     9961 cgattgcgcg ccaaagtact caggcatcgt cgcaaatcgc ctggtggtgg cataccgact
    10021 tcttcacgca attttccgga gcatccacca tcgtccgcca ccgatacgat ggcggtaatc
    10081 gagcagggca gcaggcgaag cgcggccaac agcgctgctt ggccgtgtcc tccacccatc
    10141 gtaacaatct ttggcggggg gatcgtaggc atggaatcgt tgaacgcaaa gcataccggg
    10201 cttatcccct cgttgtcatg ttccgatcac cctacggaaa gcgttttcga ggttgctcaa
    10261 acgttcgggc caccatcgaa aggggaagga cgagttgacg cgcgtcgcag gcagagtgaa
    10321 acttcctgta tccatgcttc gttggccaat cctacttggt gtgctgttat ccacgacctt
    10381 tgcggcggcc caatcgaccg acacgcgcag tgaattggag cgtgtccggg cagcggctga
    10441 tgaattcgat gcgggccgtc gagctttcac cgccgaggat tactccacgg ccgctgagca
    10501 tttcgaaaag gcagaccggg acgctccggc gcccgaggca ttgcgcatgg caatccgcgc
    10561 gcgtctcgcc gccaatcagc ccgaccgggt tgccacgctg gcggctgccg ctctttcgcg
    10621 gtacccaaac gatgaagcca cggtggcgtt ggcaaaagaa atgctcgaga aaatgcccaa
    10681 acgcctgcat cgaatcaccg ttcaatgcaa cgaaccttgt tccttgatgg tcgatcgcag
    10741 gctcgtacct ggtgtggcag cgaccgaatc gaccgtatat gtcgagccag ggcaatatga
    10801 aatcacggcg ggatggagcg cgggacgggc tcgaagcgag cgtatcgatg cgttgcctga
    10861 aaacgcgagt accatggtat ttcaagctcc accactgacg acggcggatc cggaacc
//
LOCUS       DAOSOR010000136        10862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6032_length_10862_cov_4.214120, whole genome shotgun sequence.
ACCESSION   DAOSOR010000136 DAOSOR010000000
VERSION     DAOSOR010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 10862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10862
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6032_length_10862_cov_4.214120"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1495)
                     /locus_tag="PK710_09440"
     CDS             complement(<1..1495)
                     /locus_tag="PK710_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09983.1"
                     /translation="MGSHDQEIDGEWTNHGIEEMDDLEEVAVEAADDEVPSEEVLLKP
                     QIPDEATGIMEIPFAAYSTDNSPNDDDDQGDPSEGPEEPLSAVPDIAEEFIVEESMAE
                     EFSAVEPMAEESMAEEDVDIAPQAPIQSEPARESKPIAVDLERTIDEAMHSPEREELW
                     DRAEQLARQADRMDAVGAAYQKLAASELETSVALALLDRAVQFFDEWMNDLDGVATLL
                     HRVLDYDPTARWAFDRISLKLTVEARWDELLALYDRVLTNTSDLPTRLALLDEAAAVA
                     KDCAGNPERAIDYLQKVFEAQPSDTRTASALERLLKLQKRHEDLIGFWNQRLQVLTGQ
                     EALSTRQQIASCWLEDLQDPNGALEAVEPLLDDPRTASAAGSLLEMIVSSGATTPEIR
                     ARALKLLGESYDETERWHLVIDALETAIGHVSSDERAFIHQQIARRLIGHDALEQALD
                     HLAALLVTERDVWTDKRVQLVLRGEFKEKVHGFYPVLDREQGYRLLHL"
     gene            complement(2061..2384)
                     /locus_tag="PK710_09445"
     CDS             complement(2061..2384)
                     /locus_tag="PK710_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09984.1"
                     /translation="MTIRTPYKEESRDNGLGACSDEAMNVRITVALQGRVVAIDQVTD
                     RRISDPFVQAGKQIAIKLERLRCPVHGKTASNVRLHFDVSGAVDLQYDSCCEKLGTVI
                     GRALG"
     gene            complement(2381..2812)
                     /gene="arfB"
                     /locus_tag="PK710_09450"
     CDS             complement(2381..2812)
                     /gene="arfB"
                     /locus_tag="PK710_09450"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HOT09985.1"
                     /translation="MSEDLFIADGLFIPAAELRWVAVRSSGPGGQNVNKVSTKVELRF
                     DLEGSTVLPEPVRTRLRALARTRCDAEGWILITSQRSRSQETNLQDARQKLAAFIRRA
                     LTPPIERRPTTPTRGAKERRLRAKKELADKKAGRRKVVPSE"
     gene            3080..4771
                     /locus_tag="PK710_09455"
     CDS             3080..4771
                     /locus_tag="PK710_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09986.1"
                     /translation="MRLTSLLPLLALPFALACSAADSDLPIDPQDQADSTEQAYSGYT
                     ANPSLILNGQANLEAFSENRDFAVFSVDNGSSPCKSLYLLNTNTAKTTLLTADSFCPQ
                     FYSHPLAVVDTLGQRVAYWTWSETHQTVRLMFWQSGAVPVVVEEQPEQAVEGRVKELY
                     FGASHLLYGIPGLNNRFIVKVFNISTGLRSTLSTEVFVGAEPLFDPTGQSLLLTETND
                     DDDSILTLYHLASGSSSVIDSGKQWEVKPSPRGQFVASQSQCVFEEADVRCRLRLFDW
                     NSQQVFEAPDADVSKIVGFAADDTALAYIAGGKLQIWNLSAGTIDQIPGANTCWAATT
                     PSSSRVVYVTDCNQGSKGMEGELRVVDLQEPGLPKTLGHRVSPGDNMLVMSRDGNRVA
                     AVRESIPWTYVDLWTLSTGKKESTNMTNGVLYTRFSNDGSKFIYAKSTATPGLCAPYY
                     VCGYLSLRGYDFTRTTLKDQHIEGSMLAMLDLEGMLGNWQTRPADKSLLQSPDGQRMT
                     YSTTNGSATLGSTFVLQSNTLQNRKVLGNWRPILITDQYLLVTSMKGSGVYRVSL"
     gene            complement(4856..5752)
                     /locus_tag="PK710_09460"
     CDS             complement(4856..5752)
                     /locus_tag="PK710_09460"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT09987.1"
                     /translation="MTNPRRAYAFGLMTVLLWSTVATAFKLTLRHLEPVQMLLVAHVT
                     SIVVLAVILWVRGNIKALGSMTKRQALTSLGLGLINPFLYYLVLFEAYHRLPAQEAQS
                     LNYTWAITLSLLSVPLLKQRITARQLVAAALGYAGVVVIATRGDVLSLRFSDTFGVLL
                     ALGSTVLWSIYWIFSKRDTREPVVALLSSFLLGLPFVVGACLWLSTPWITNVRGLLGA
                     IYVGVFEMGAAFVLWLEALRASEHTARVSQLIFLSPFLSLVFIRLGLGEPIVRSTYVG
                     LGLILMGLMVQKGAKASPEGQG"
     gene            5872..6639
                     /locus_tag="PK710_09465"
     CDS             5872..6639
                     /locus_tag="PK710_09465"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HOT09988.1"
                     /translation="MDTIPIFNDISTNRSLSLAPLIPLAHAERCRMDPPAPPSAAAPP
                     ARILWLAVIVIASVSTYAIALLTGHVRPDTIQTLVADGGPSAMAVYVLAVIIAELLWF
                     PRMWGLLAGGILFGPVLGAVLSILGDSMAAVLCYFIARGTGRAWVSAQLEKKPSARRV
                     VHILAERRGAVTIALLRVCPIAHYTLVSYGAGVAGVSPRSFLLGSTIGIIPGAIVYPI
                     VGDAALRPTSPMFLGSLAVVLVFLVVTLVAARRTFKP"
     gene            complement(6683..8119)
                     /locus_tag="PK710_09470"
     CDS             complement(6683..8119)
                     /locus_tag="PK710_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOT09989.1"
                     /translation="MDLETLEHIARQLDPDAPQRASLASYVMEYAERHWSGVEGALAF
                     VQQSHPGAGLAEHAFREEGRPISEVLSILEREVDTPGAHGGSGRHFAFIPPSTQIAGA
                     LGDYLAASTNKYAGYFFAGPGAVRMENLLLHWMARELGFPQGTHGNLPAGGSLAHIIG
                     ICAAREAAGFKAADTPKICVYLSDQAHHCIAKALRVAGLGEARLRVIASDDHFRMRMD
                     RLQAAIQQDVAAGNRPWLLAPTAGTTNSGAVDPLEECAHIAARYGLWMHTDGAYGAFF
                     ALCEEGKQRLVGQHQSDSLVADPHKGLFCPFGIGAVLVKHLDPLRRAHAYAADYLPTP
                     PDDLNELSPTESTLEFSKHARALRLWLPLMLFGVSPFRAALEEKLRLAQYAWEKLQSL
                     KGFDPVAAPQLSVMAFRLLPPSGDPDAFQQDLLANLYREGRVFLSSTRLGDRLCLRLA
                     VLALRTHREHVDEAIDCLARAYKQAITN"
     gene            8441..9811
                     /locus_tag="PK710_09475"
     CDS             8441..9811
                     /locus_tag="PK710_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09990.1"
                     /translation="MIPSISKYSLFGILGLAGLVVIAGVSCGSDDSDDSSGPGASTGK
                     DAGSGGTGGSSGSGGASGTGGGAGTAGTGGSAGTSGIGGGAGASGTGGGAGAAGSGGS
                     GLTCPAGVADCDLDPTNGCETDIFSSPSDCGACGKKCDGLCLSGDCAPFELLADSQHW
                     PPYNHGIVFSDTEVFWVSNADKNQSAEKYRVQKNSIISPSTPVSIITNMRELDQIAIS
                     PKRLYFVGSRRDRILFASDFDGSNLTQEQTNVFGVQYSANRYYYSSNYNGAAYLKWKN
                     VLTDTVGTTYEKKSPTWNGESIGYSMQAVAGYIIFAEIDKNSYRIYHDSPSDLMQQGS
                     GSVTRLRYTTGWELLWVESAEAQGQNDVLKFAENKNPPVVVTQAPVIVDFAYQDPLVY
                     VSTVESNYTEPKLSVIDMEAQTTTTYILPGLAASLEINGDYLYFFVNSRESFVDYRLV
                     RLKLPI"
     gene            complement(9936..>10862)
                     /locus_tag="PK710_09480"
     CDS             complement(9936..>10862)
                     /locus_tag="PK710_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOT09991.1"
                     /translation="SDTLQPAQTQYGERLTLVRADVRTLPLETFSGVDAVIDLAGISN
                     DPSCELAPSLTRQVNYEAAVTTMRHAQRAGVKRYILASSCSVYGRGEGVGLTESSPLN
                     PVSLYARCKADAETALLSLRSSDFCPTILRLATVFGLSPRMRFDLAINVMTKNAYTNG
                     KITVDGGGKQWRPFVHVADVAETMVFMLHAEAHNVDGQIFNVGHDDNNVRILNLAYRV
                     RDAIPGTQIVMAPTDPDLRDYNVRFEKLWGLLQDRQFRTIEYGIEEVLGALRSGFVDP
                     DERRWYTLAQYRFLLDVERTYQEVVLEGRVLS"
BASE COUNT         2346 a         3204 c         3003 g         2309 t
ORIGIN      
        1 ccaagtgcaa tagccgatat ccctgttccc gatccaggac cggataaaag ccatgtactt
       61 tctccttgaa ttcaccgcga agcacaagtt ggactcgctt gtccgtccaa acgtcgcgct
      121 ccgtgaccaa caacgccgcc aaatgatcaa gagcttgctc cagggcgtcg tgaccgatca
      181 gccgacgagc gatttgttga tggatgaacg cacgctcgtc ggaagacacg tggccaatcg
      241 ccgtttccaa cgcgtcgatc accaagtgcc atcgctccgt ttcatcataa ctttcgccca
      301 agagcttcaa cgcacgagcg cgtatctccg gggtcgttgc cccggaactg acgatcatct
      361 ccaacaaaga cccggccgcg gaagctgttc tcgggtcgtc gagcaagggc tctaccgcct
      421 cgagcgcacc attggggtct tggagatcct ccagccaaca agaagcaatc tgctgtcgtg
      481 tactgagcgc ttcttgcccc gtcagaacct gaagccgctg gttccaaaag ccgatgagat
      541 cctcatgacg cttctgcaat ttgagcaacc gctcgagcgc cgaggcggta cgcgtgtccg
      601 acggttgggc ctcgaatacc ttttgcaagt aatcgatcgc acgttcggga ttgcccgcgc
      661 aatccttggc taccgcagca gcctcatcga gcaatgccaa tcgagtcggc aaatccgacg
      721 tattggtcaa aacccggtcg tataaggcga gcaactcgtc ccatcgtgcc tcgaccgtaa
      781 gcttgagaga aatgcggtcg aatgcccatc gcgccgtcgg atcgtaatcc aaaacgcgat
      841 gaagcaacgt cgccaccccg tccaaatcgt tcatccactc atcgaaaaac tggactgctc
      901 gatcgagcaa ggccaacgca accgacgttt cgagttccga ggccgcgagc ttttggtacg
      961 ccgcccctac cgcatccatg cgatccgctt gtcgagccaa ctgttccgcc cgatcccaaa
     1021 gctcttcccg ttccggcgaa tgcatcgcct cgtcgatggt ccgttcgagg tctacagcga
     1081 taggcttcga ctccctcgcc ggttcggatt ggattggcgc ttggggcgcg atgtccacat
     1141 cctcttcggc catcgactcc tccgccatgg gctccacggc gctaaactcc tcggccatcg
     1201 actcctcgac gatgaactcc tccgcgatat ccggaacagc cgacaacggt tcttcggggc
     1261 cctcggatgg atcgccctgg tcatcatcat cattaggcga attgtccgtc gaataagccg
     1321 caaacgggat ctccatgatt cccgtggctt cgtcgggtat ctgaggtttc aataagacct
     1381 cctccgacgg cacttcgtca tccgccgctt ccacggcgac ctcttcgaga tcgtccattt
     1441 cctcaattcc gtggttcgtc cactcaccgt cgatttcttg atcgtgtgaa cccatgacaa
     1501 ggtcggcagt ctaatcgatg gacaagacaa tggggtatgg aatgcggcaa acccgtggat
     1561 atggtgctta cataagggca atgaatagcc tcatcaccct gctgaccgag gcgaagtggg
     1621 cgggcgttcg tgatatccaa ttgctataac tatttgaaaa taaagttgaa aatagaaggt
     1681 cgggacctgc ttcgaagagc ctccattcac cgtgtcgccg cagcccacga gttcggtact
     1741 cccgctagcc cgtcatgggc ccagcccatc cacgatagcg tgctcgtcga aataccgggc
     1801 cgtcgcctta ccagccctga accaagctca cggtgccgtc agtcgccaga accctcgctt
     1861 gtagaatccg tcgcggagga taaaggccgc agtcgcttcg catcccggtg ggaaagcatt
     1921 gggtctcccg tgcttgcgcg cgaacgggcg atccagaaaa tgacaagggc agccgaaacg
     1981 atacccacga cgagaaccat tccaacgagc gctatcaata tcaattcgag aactcccagt
     2041 gtcgaaatca tccttgtcaa tcacccgagc gcgcgaccga tgaccgtacc gagcttttca
     2101 caacacgagt catattgcag gtccacggca cccgagacat cgaagtgcaa tcgtacgtta
     2161 ctagccgtct tgccatgaac agggcaacga aggcgctcca acttgatggc gatttgcttt
     2221 cccgcctgga caaaagggtc agagatgcga cggtccgtca cttggtcgat cgcgaccact
     2281 cgaccttgaa gcgccaccgt gattcggacg ttcatcgctt catccgaaca tgctccaagg
     2341 ccgttgtcac ggctttcttc cttgtaaggg gtacggatcg tcattccgac ggaaccactt
     2401 ttcggcgtcc tgcttttttg tccgcaagct cttttttagc gcgcaaacgt cgttctttgg
     2461 ctccacgggt aggggtcgtg gggcgacgtt cgatgggggg tgtgagcgcc cggcggatga
     2521 aggctgccaa tttctgccgc gcgtcttgca gattggtttc ttgagaacgc gagcgttggc
     2581 tggtgatcag aatccacccc tcggcatcgc aacgtgtgcg agcgagcgct cggagacgtg
     2641 ttcgtaccgg ctccggcaac accgtggaac cctccaaatc gaaccgaagc tccaccttgg
     2701 tggagacttt gttgacgttc tgtccgccgg gcccggagga acgaacggct acccaacgca
     2761 attcggctgc cggaatgaaa agaccgtcgg cgatgaacaa gtcctcggac acgccccgac
     2821 catgcttggt tttggctctg atcgcaagca gggggctgac aagggcagct cggaagttgc
     2881 tggatgaata aaccagccgc cgcaaccggg gtaccaaagc cggtgacggt atatggtgat
     2941 ttgaccaaat atcgtcagtt tcgttttgaa cttcagccca ctcggatcga aaaatgacga
     3001 tgaatccgca attgagcgga tggaacggaa tgtgctaacg tgcgtaccca aacccacata
     3061 tcggatatca ggaaatacga tgcgactcac ctccctgctt ccccttctcg cccttccttt
     3121 cgccctcgct tgcagcgccg cggattccga cttgcccatc gatccgcaag accaggccga
     3181 ctccaccgaa caagcgtatt ccggctacac cgccaacccc tcgttgatcc tcaacggcca
     3241 agccaatctc gaagcctttt ccgaaaaccg cgatttcgcc gtcttcagcg tcgataatgg
     3301 ctcttcgcct tgcaaatccc tgtacttgct caacaccaac acggcaaaga ccacgcttct
     3361 gacagccgac agcttttgtc ctcagtttta ttctcacccg ctggccgtcg tcgataccct
     3421 cgggcagcgg gttgcctatt ggacatggtc ggaaacccac cagacggttc gcctcatgtt
     3481 ttggcagtcg ggtgcggtcc ctgtcgttgt cgaagaacaa ccggaacaag ccgtggaagg
     3541 ccgggtcaaa gaactctatt tcggagcgtc tcacctgtta tacggtattc cgggcctgaa
     3601 caaccggttc attgtcaaag tattcaatat ttccacgggc ttacgctcca ccctgtccac
     3661 cgaggtgttc gtgggcgcag aacctctgtt cgacccaacc ggccaatcgt tgcttctcac
     3721 cgaaaccaac gatgatgacg atagcatctt gaccctctac caccttgcct ccggctcatc
     3781 ctccgtcatc gattcgggca agcaatggga agtgaagccc agcccccggg gccagttcgt
     3841 ggcttcccaa tcccaatgcg ttttcgagga agctgatgtt cgctgccggt tgaggctttt
     3901 cgattggaat agccagcagg tattcgaagc tcccgacgcg gatgtttcga aaatcgtagg
     3961 atttgccgcc gatgacaccg ctttggcgta tatcgctggt ggaaaactgc aaatctggaa
     4021 cctttccgcc ggtaccatcg accagattcc cggggccaac acctgctggg ccgctaccac
     4081 accatcgtcg tctcgggtcg tgtatgtgac ggattgcaac caaggctcaa aaggaatgga
     4141 aggagaattg cgggtggtcg acctccaaga acccgggctc cccaaaacct tggggcatcg
     4201 cgtgtccccc ggcgacaata tgcttgtgat gagccgcgac ggaaaccggg ttgccgccgt
     4261 tcgcgaatcc attccatgga cctacgtgga tctctggacc ttgtcgacag gcaaaaaaga
     4321 gtcgacgaat atgacgaatg gcgttttgta cacgcgcttc agcaacgatg gcagcaagtt
     4381 catctacgca aaatcgactg cgacgccggg gctttgtgcg ccctactacg tttgtggata
     4441 tctctccctt cgcggttacg atttcacgcg cacgacactc aaagatcaac atatcgaggg
     4501 aagcatgctc gcgatgctcg acctggaggg gatgctggga aattggcaga cccgacccgc
     4561 ggacaagtca ctcttgcaga gtcccgacgg tcaacgaatg acctattcca cgaccaacgg
     4621 cagcgctacc ctcggctcga ccttcgtgtt gcaatccaac accctgcaaa accgcaaggt
     4681 gctcggcaat tggaggccca tcctcatcac tgaccaatac ctgctcgtca cctccatgaa
     4741 gggctcgggc gtgtatcgcg tatccttata gtcgtccccc tggaatcccc gccgctcccc
     4801 gtcctgacga gatgatgagc cataagcttc gtaccgtagc atatggctcg gaagctcagc
     4861 cctgaccctc cgggctggct tttgcgcctt tctgcaccat caagcccatc aaaatcaagc
     4921 ccagcccgac atacgtggaa cgcactatcg gttctcccag gcccagacga atgaacacga
     4981 gcgacagaaa gggagagaga aaaattagtt gactgacgcg cgccgtatgc tccgaagcgc
     5041 gcaaagcttc gagccagagc acgaaagctg cgcccatctc gaacaccccc acgtaaatgg
     5101 cccccaacaa cccccgaaca ttcgtgatcc agggcgtaga caaccaaaga cacgccccca
     5161 ccacgaaggg caatcccagc aaaaaacttg ataataaagc caccaccggt tcgcgtgtgt
     5221 cgcgtttgct gaaaatccaa taaatcgacc aaagtaccgt gcttccaagc gccagcaaga
     5281 ccccgaacgt atcggaaaac cgcaacgata acacatcgcc tcgcgtcgca atgaccacga
     5341 ctcccgcata acccagggcc gcggccacga gctgacgcgc cgtgatgcgt tgtttcagca
     5401 aaggaacgga cagcagtgac agcgtgatgg cccacgtgta gttcaacgat tgcgcctctt
     5461 gcgccggaag acgatggtat gcctcgaaaa gcaccagata atacaagaaa ggattgatca
     5521 acccgagccc gagggaagtc agggcctgac gcttcgtcat tgaccctagc gccttgatat
     5581 tgccccgcac ccatagaatt accgcaagca ccacgatgct cgtgacgtgg gccaccaaca
     5641 gcatttgtac cggctcgaga tgccgcagcg tgagcttgaa cgccgtggcc acagtcgacc
     5701 acaacaaaac cgtcataaga ccgaaagcat aggcccttcg aggattcgtc atgctcctcc
     5761 agcctatcgc gaatcgaccc accctgggca aactcgctcc cccacctccc ttttcgggct
     5821 ccgcggtttc gaaatgccag gcaaccacgg ccttcccacg ggtcccggca catggatacc
     5881 atcccgatat tcaacgatat ttccacgaat cgctcgctgt ctcttgctcc tcttatcccg
     5941 ctggcgcatg ctgagcgctg tcgcatggac ccgcctgctc ccccctccgc cgctgcccca
     6001 cccgcgcgca tcctctggct tgcggtcatc gtcatcgctt ccgtcagtac ctacgccatc
     6061 gcgctgctca ccggccacgt tcgtcctgac acgatccaaa ccctggtagc cgacggcggc
     6121 ccgagcgcga tggcagtcta tgtgctagcg gttatcatag cggaattatt gtggtttccc
     6181 aggatgtggg ggttgctcgc gggcgggatc ctttttgggc cggtccttgg tgccgtcctt
     6241 tccatccttg gggattcgat ggccgccgtt ctctgctatt tcatcgcgcg cggaacagga
     6301 agggcgtggg tgagcgcgca gttggaaaaa aaaccttccg cacggcgggt ggtgcatatt
     6361 ttggccgaac gacggggtgc cgtgacgatt gcgctccttc gggtgtgccc gatagctcat
     6421 tatacgttgg tgagttatgg ggcgggggtt gcgggggttt ccccacgttc ctttttgctg
     6481 ggaagtacga ttggcatcat ccccggagcc atcgtctatc ccatcgtggg cgatgctgct
     6541 cttcgcccca caagtcccat gttccttggg tctctggccg ttgtcctcgt ttttttggtg
     6601 gtaaccctgg tagccgcgcg tcgcaccttc aagccttagg ttcccttgca ggtcttttgc
     6661 aaggttgaaa aatcttgtaa tttcaattag ttatggcttg cttgtacgcc cgtgccaggc
     6721 agtcgatggc ctcgtctacg tgctcgcgat gcgtgcgcag ggctagcacg gcaaggcgca
     6781 aacaaagccg gtccccaagc cgggtcgaag acaaaaacac ccgtccttca cgatataggt
     6841 ttgccaatag gtcttgctgg aaagcgtcgg gatctcccga aggcggcaac aatcggaagg
     6901 ccatcacgct caattggggt gctgccacgg gatcgaaccc cttcagggac tgcagttttt
     6961 cccatgcata ttgcgccaat cgcagttttt cctccagggc ggcgcgaaac gggctcacgc
     7021 cgaaaagcat cagcggtagc caaagacgca gtgcccgggc gtgtttgctg aactccaggg
     7081 tggattcggt ggggctcaat tcgttgaggt catcgggggg agttggcaga taatcggctg
     7141 cataggcgtg agcgcgacgc agggggtcga ggtgtttgac caagaccgcg cctatgccaa
     7201 acgggcagaa caggcctttg tgggggtcgg ctaccaagct atcgctttgg tgttggccca
     7261 cgagccgttg tttcccttct tcacaaagcg caaaaaaggc tccgtaggca ccatccgtat
     7321 gcatccacag gccgtatcgg gcggcgatgt gcgcgcattc ttccaaaggg tccacggcac
     7381 cgctattggt ggtacccgcg gttggtgcca ataaccaagg tcgattgccc gccgccacgt
     7441 cttgttggat ggcggcttgc aaacggtcca tgcgcatgcg gaagtgatca tcgctggcga
     7501 tcacccgcag tcgagcctcg cccaatcccg ccactcgcaa cgctttggcg atacagtgat
     7561 gtgcctggtc ggaaaggtag acgcagattt ttggtgtatc ggcggctttg aaaccagcag
     7621 cctcccttgc ggcgcaaatt ccaatgatat gcgcgaggct acctcccgcg ggcaggttgc
     7681 cgtgcgtgcc ttgcggaaag cccaattccc tcgccatcca atgaagcaga aggttttcca
     7741 ttcgtacggc cccggggccg gcgaaaaagt atccagcgta tttattggtg ctcgcggcca
     7801 agtagtcccc caaggccccg gctatttggg tagacggcgg aatgaaagca aaatggcgcc
     7861 ccgaaccacc gtgcgctccc ggcgtgtcca cctcccgctc gaggatcgac aacacctcgc
     7921 tgatcgggcg tccttcttcc cgaaacgcgt gctcggcaag tcccgccccc ggatgggatt
     7981 gctgaacaaa cgcgagggcc ccttcgacac cgctccaatg ccgttcggcg tattccatga
     8041 cataagaggc aagtgaggct cgttgggggg cgtccgggtc caactgacga gcgatatgtt
     8101 ccaacgtttc gagatccatg ccttctccga tcgagcgagg gcgaaggcgg tattcgagca
     8161 ttgcgggggg aatggggcaa gatggtttcg agaagaaccg ccttatgcga tgccgtggca
     8221 acctcaccga gacaacggtt ccgtcgatac ggaaacgcag gggcgaggtg ggggaaatgg
     8281 tcgtgcccga atgctccatg taagcataaa ctactgaaat atattgctat ttcagttctc
     8341 ccgcttccgc tctcccattt tcgcagccgg tttgcaaaga caggaaaaac ccgtgatgga
     8401 aacgattgac atcgcgtggc cgtgaggtca tatcaatcgg atgataccgt ctataagtaa
     8461 atattcgttg tttggtattc ttggattggc tggactggtg gttattgcgg gcgtgtcttg
     8521 tggctccgat gattccgacg attcatcggg gccgggagcg agcacgggca aagacgctgg
     8581 tagcgggggc acaggcggct cttctggtag tgggggggcc tctggcacgg gaggcggtgc
     8641 gggaaccgct ggcacgggag gcagtgcggg cacatcgggc ataggtggtg gcgcgggggc
     8701 ttccgggacc ggcggcggcg ctggcgcggc tggctcgggg ggcagtggtc tgacttgtcc
     8761 tgctggagtc gcggattgtg atctcgaccc gaccaacgga tgcgagacgg atattttttc
     8821 ttctccgtcg gattgcggag cttgcggcaa gaaatgtgat ggcttgtgcc tgtcgggcga
     8881 ctgcgcaccc tttgaactac tggctgattc gcaacattgg cctccctaca atcacggtat
     8941 cgtgttcagc gataccgagg tattctgggt cagtaacgct gacaaaaatc agtccgcgga
     9001 aaagtaccgc gtacagaaaa attcgatcat ttcgccctcg acgcccgtca gtatcatcac
     9061 caatatgagg gaactcgatc agatcgcgat cagcccgaaa aggctgtatt tcgtggggag
     9121 tcgacgggat cgaatcctgt tcgcgagtga tttcgacggt tccaacctca cccaggaaca
     9181 aacgaatgta tttggggtgc agtacagtgc gaatcgctat tattactcat cgaactacaa
     9241 cggagccgcc tatctgaagt ggaaaaacgt actgacggat acggtaggca ccacctacga
     9301 gaaaaaatcg ccgacgtgga acggtgagtc catcgggtat tcgatgcagg ccgtcgccgg
     9361 atatatcatt tttgcagaga ttgataaaaa cagctatcgc atttaccacg actcaccatc
     9421 ggatctgatg cagcagggca gcggcagtgt gactcgattg cgctacacga ccggctggga
     9481 gttgttatgg gtcgaaagtg cggaggcaca agggcaaaac gatgtgctga agttcgcgga
     9541 aaacaagaat ccgcccgtcg tggtgacgca agctcccgtc atcgtcgact ttgcatacca
     9601 agatccgctg gtctatgtgt ccacggtcga aagcaattat accgagccga aactaagcgt
     9661 tatcgacatg gaggcccaga cgacaaccac gtacattctc ccaggcctcg cagcctcgtt
     9721 ggaaatcaac ggggactatc tttacttctt cgtgaatagt cgtgagtcct tcgtggatta
     9781 tcgcctcgtt cgcctaaagc tgccgatcta agggagcagg ttgcagcgtc ttcttgggtg
     9841 gctctcaggc gagacaagag ccaaccatct ccgtcgacag tatgtaacct attgataata
     9901 aaggataata tgctatcgct ttgggctggc agcgcttacg aaagcacccg tccttcgagc
     9961 actacctcct ggtaggttcg ctccacatcg agcaagaaac ggtattgggc gagcgtgtac
    10021 cagcgtcgtt catcggggtc cacgaagcct gatcgaagcg ctccgagcac ctcttcgata
    10081 ccgtattcga tcgtgcgaaa ctggcggtct tgcagcagtc cccaaagttt ttcgaagcgc
    10141 acgttgtaat ctcgcaggtc gggatcggtg ggagccatga cgatttgggt tcctgggatg
    10201 gcgtcccgca cgcgataggc cagattcaaa atccgcacgt tgttgtcatc gtgtccgaca
    10261 ttgaagattt ggccatcgac gttatgcgcc tccgcgtgca gcatgaagac catggtttcg
    10321 gcgacatccg ccacgtgaac gaaaggccgc cattgtttgc cgccgccatc gacggtgatt
    10381 ttgccgttgg tgtaggcatt tttggtcatc acgttgatgg cgagatcgaa acgcatgcgc
    10441 ggcgacaggc cgaagacggt ggcgaggcgc aagatggtgg gacaaaagtc ggaggaccga
    10501 agggatagca gcgccgtttc cgcatcggct ttgcagcgtg cgtacaaaga gacggggttg
    10561 aggggcgagg attccgtaag acccacgcct tctcctcgtc catacacgct gcacgagctt
    10621 gccaatatat agcgtttcac gcccgctcgt tgggcatggc gcatcgtggt tacggcggct
    10681 tcgtagttga cttgacgagt cagggaagga gcgagttcgc acgacggatc gttggagatg
    10741 ccggccaggt cgatcaccgc atccaccccg gaaaatgtct ctagcggcag agtccgcacg
    10801 tcggcgcgaa ccaacgtgag ccgttcgccg tactgcgttt gcgcgggctg cagggtatcg
    10861 ct
//
LOCUS       DAOSOR010000137        10857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6041_length_10857_cov_4.277541, whole genome shotgun sequence.
ACCESSION   DAOSOR010000137 DAOSOR010000000
VERSION     DAOSOR010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 10857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10857
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6041_length_10857_cov_4.277541"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="PK710_09485"
     CDS             complement(<1..1132)
                     /locus_tag="PK710_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HOT09992.1"
                     /translation="MTTSYAPGQILVDKYQVEKVIGEGGMGVVVAALHMHLQRRVAIK
                     FLLPGMLENEEVVARFLREARAAAMLESEHVAKVIDVGELPDGSPFMVMELLSGMDLA
                     TLVQERGPLSVQDAVEYVIQGCEAIAEAHRAGIVHRDLKPSNLFLATRKDGTSIVKVL
                     DFGISKVHSKTGSMALTRDGAMMGSPTYMAPEQIKNSKNVDTRVDVWALGVVLYELLT
                     GQPPFDGEGYHEVLSQVLLEPPPDLSTRRPDIPAELEAAVLRCLEKDPEKRFSSVTEL
                     AKTLQPFVESAGARRAFESISRWDIIAESKTMLEGKPSGAMHAGSTTDVAVTSTSSEE
                     KTPLSRESNKGTLIGAAIVLVLALVVGASAWMMGRKEASTAGA"
     gene            complement(1548..1772)
                     /locus_tag="PK710_09490"
     CDS             complement(1548..1772)
                     /locus_tag="PK710_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09993.1"
                     /translation="MTSERALDVRAAAKGSHLLRRPQKTPARRLCATRVFCGPATASR
                     AGEDGEGKVRSCGLFSSSSLPLGFWVGATG"
     gene            1973..2045
                     /locus_tag="PK710_09495"
     tRNA            1973..2045
                     /locus_tag="PK710_09495"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2007..2009,aa:Glu,seq:ttc)
     gene            complement(2430..4157)
                     /locus_tag="PK710_09500"
     CDS             complement(2430..4157)
                     /locus_tag="PK710_09500"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09994.1"
                     /translation="MRRFLPVLSLVIAACGGPSPQPSVPPAPMMPEKQQQPPPPLPPE
                     PPALTIAPDIDARLAKFATVVMDFDDSTISSDDKKVLAALVDASRMMHELFMDQIDPQ
                     IPSIRSQLLSHPTALAYFDVMAGPWDTLNHDEPFVGTKARLPGGAFHPADMTKDEFEA
                     FVKAHPDQKDDFLGYFTVIRRHDGALEALPYSKAYADKLGPAAQALRRAASLTNEPTL
                     RAFLEKRADAFLSNEYRESDRAWMDVKGPIEVTIGPYETYADQLLGLKASFEAFVSLR
                     DVEESRKLETIGKQMNALERNLPMTTKQRARMAERHQGSPIDVVQLLCNAGQAGVQTV
                     AYNLPNDEHVRKEKGSKKVMMKNVLRGKFDHIVIPITERVLVADQRELLDFEAMFSYI
                     LMHEVAHGLGPGIITLADGSKAEVNKMLREQYGAIEEAKADIAGLVSAQYLIDRGLLP
                     RAYDKPIYVAYLATVFRQVRFGVGEAHGRGAVASFNFLREQGAITYDNQAERFGIDFK
                     KVKAAVRKLAEVYLTLEVDGDYEGAKQFLAKYANVSPEMKKALDSLGSGIPVDIRPQF
                     AIYEIIKTW"
     gene            complement(4226..5680)
                     /locus_tag="PK710_09505"
     CDS             complement(4226..5680)
                     /locus_tag="PK710_09505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOT09995.1"
                     /translation="MTSPLANLEPKHLWQHFDQIRQIPRPSRHEEAIRTHVKAWAERR
                     KFEWVEDEVGNVCVRVPGTQGHENASVVIIQGHLDMVPEKDSDFEFDFTKDPIACRVD
                     GDWVVADKTTLGADNGIGIAAAMAAADDPDVVHGPLELLFTIDEETGLTGATNLDGSI
                     LKGKIMLNLDSEEDGVLFVGCAGGCTSEIQFDLPRVSLPSGYVGMKLEVTGLKGGHSG
                     LNIIDNRANSIKVLCRVLGRWLDQHDVFIESIDGGNKHNAIPREATAVVAIPSHFVES
                     AKTIAAEEQAYILTEFKTIDGGLTIRADKAEVKKVASAEASRRLVAMLTALPDGVSAM
                     SRDIAGLVETSSNLAIASTDGDKIAITTSSRSSVASALRGVLDEAKAVVALAGGVYTE
                     VGAYPAWQPNMDSKLLGVCQRVYKQLFGKEPKVAAIHAGLECGIIGEKIGSDADMISF
                     GPELQGVHAPGEKVKISTVVKFWDFYKKVLADLA"
     gene            complement(5849..6673)
                     /locus_tag="PK710_09510"
     CDS             complement(5849..6673)
                     /locus_tag="PK710_09510"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOT09996.1"
                     /translation="MDFFFDWLRTTTWATSADWGELAYWEVTLRLAAATILGGAIGFE
                     RERRDRAAGLRTHALVSVGAALAMIVSTYGFPLPPAGSSGSLDPSRIAAQVVSGVGFL
                     GAGVIIFRDNTVRGLTTAATVWAVAGVGLAAGGGLYVAALVGTAFMLLVQAGLKPIER
                     KIFAKYAKQHRILLTVDHGVDALEGIHFATAETPVRLRSLQFDHARGSDLDVVELTLL
                     ADTQQQVLDLLTRLREVKSVHSIAYGRRSSIVQRRVRDEGHDFGHDEDDDEGNDQA"
     gene            complement(6782..8554)
                     /locus_tag="PK710_09515"
     CDS             complement(6782..8554)
                     /locus_tag="PK710_09515"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT09997.1"
                     /translation="MPKHPKSDRILGYPPGFRYIWLASLPSIPTAFMNVSGGTMRSIA
                     TLTVLSMGLGVCACKQPTQGSEIVGEASSAQATPVAASVPPPAVSSVRPTLSTTTPEP
                     SAFEPCPQGMALVGPQLCVDQWEASLVDEHGSPISPYLPVGTKKVVAVSRPDVVPQAH
                     ISLFQATRACEAAGKRICTTQEWLDACQGVEKPKRTYPYGQKHRARACNDRTRIHPVS
                     LVFPGRRRHDAVTLNDARLNQQPDTLAKTGAYPECKTPEGVFDLVGNLLEWTKGPTRP
                     LLMGGHYVDSVVNGQGCHYVTPDHKEHYHDFTTGFRCCKTAKTAPSPPPTTPADAVSP
                     PASESVNPPAVQPPARSTRRDEPRSFHNAFGFLPHVSPPPYESRKAPCPVDMVLVQGE
                     RCAVVEQVCQGFIDPAGMPERACGQFEKTVCKGPRRQMRFCIDRYEFTSPGERLPLVN
                     VAFPEAEYLCRKMDKRMCFEQEWEFACEGPEAWPYPYGYVRDGARCNHDKSNLFVRGN
                     ELFDQRVAADSLPGCKSPFGVFNLVGNVDEWTQRPGNKPPNRSILRGGWWLMGRNRCR
                     AATSSHNETYAGAQTGFRCCKAAR"
     gene            complement(8547..9134)
                     /locus_tag="PK710_09520"
     CDS             complement(8547..9134)
                     /locus_tag="PK710_09520"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOT09998.1"
                     /translation="METKDNLWARASDIVEIKASPVNPWGDPTQGPGLYDKVRWSLGT
                     KAPPPPPRRTWVEVSVHEGWLIAYEGNRPVFATLVATGRGEPIEKGSNVLQSATPPGI
                     FSILGKYWTSTLAVNSTMHFDVPFVMPYFGAYAMHAAYWHDAWGEKVSLGCVNVAPID
                     ARWLFLWAEPSIPEGWHGMTVTTETGAATVVVVHA"
     gene            complement(9118..10131)
                     /locus_tag="PK710_09525"
     CDS             complement(9118..10131)
                     /locus_tag="PK710_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT09999.1"
                     /translation="MSRQILYSRLFFSATMFFGCAPDRDTPVQSLLSSTPAIPHGQAG
                     GEAEEDLPAAASAEDLEQTGNIPVLYALHRHVWIRTRPSTQAPWIGFLGLGSRVVLRE
                     PEPVRGVGCDAFYAIEPRGYVCADRRVSLDPTDPAVVAVQHIAPSRDRPWLHDYAESR
                     QAPRYEKLPTVEQQRQREFQFDSHFAMVTALRTGDHVGEIPFLLRDVDVSLSGQAIPS
                     FVDSLPRVHEHVPMVKAGSAMSYSHVFDAEGRAWVVTGDGVFVPRDRLAPYHPSTFQG
                     RNLEENDTLPIGFVKDRTQLIPSSGRRNDRSSWSRLAEACFVRIDGSNRGAKRHELHG
                     NQR"
     gene            complement(10252..>10857)
                     /locus_tag="PK710_09530"
     CDS             complement(10252..>10857)
                     /locus_tag="PK710_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT10000.1"
                     /translation="LRVLQEHEIERVGGNETLPIDVRVVAATNRDLVEACQQGSFRPD
                     LYDRLNVVPLVIPPLRARREDIPELARHFLALAARNNDRPEASIDDGGMEALCQHSWP
                     GNVRELRNIIERLVILSHDDRIGGEDVRVCLGAGPQASAGGLYRPGVPFRVLAEEAER
                     MIVQEALAHFGGQMAATARGLELERSHFYKKCKALGLRGEE"
BASE COUNT         2253 a         3243 c         3043 g         2318 t
ORIGIN      
        1 ctgcacccgc ggtcgaagct tccttccgcc ccatcatcca ggcgcttgcc cccaccacca
       61 acgctaggac gagaacaatg gcggctccga tgagggttcc cttgttgctt tcccgcgata
      121 acggcgtttt ctcctccgaa gaagtggacg tcaccgcaac atccgttgtc gaacccgcgt
      181 gcatcgcccc actcggcttg ccctccaaca tcgttttgga ctcggcgatg atatcccatc
      241 gactgatgct ctcgaatgcc cgtcgcgcac cagccgactc cacgaaaggt tgtagcgtct
      301 tggcaagctc cgtcacggaa gaaaaccgct tttcagggtc tttctcgaga catcgcaaca
      361 ccgcagcttc gagttcggca gggatgtcag gccgtcgcgt ggaaaggtcg ggcggcggct
      421 ccaacagcac ttgtgacaac acttcgtgat acccctcccc gtcgaaagga ggttgacctg
      481 tcaaaagctc atacagcacc acccccaacg cccaaacatc cacacgagta tcgacatttt
      541 ttgagttttt gatctgctcc ggcgccatat acgttggcga acccatcatc gccccgtcgc
      601 gcgtaagagc catgctcccc gttttggaat gcactttgga aataccaaag tccagtacct
      661 tcacgatgga cgtaccgtct tttcgtgtgg caagaaatag attcgacggc ttcaggtcac
      721 ggtggacgat tcccgcgcga tgcgcctcgg caatcgcttc gcaaccctgg atcacgtact
      781 cgacagcgtc ttgtacggat agaggaccac gctcttgtac caacgtggcc aagtccatcc
      841 ccgacagcag ctccatcacc ataaaagggc tgccgtcggg caactccccg acatcaatga
      901 ctttcgcgac atgctcgctt tccagcatcg ccgccgctcg cgcctcacga aggaaacgcg
      961 ctaccacctc ctcattttcc aacatccccg gcagcaaaaa cttgatggcg acccgtcgtt
     1021 gcaaatgcat gtgcaacgca gccaccacca cccccatccc tccctcgcca attacctttt
     1081 cgacttgata cttgtcgacc aagatttggc ccggggcata ggacgttgtc atcgtttcct
     1141 cgaccattca ccaaaacgac aggactgcaa acagagcata gagtagcgcc aatcccgtgt
     1201 cttggacagc ggcccccttc atccacctac gatattcatc gaggattgag cacgaaaact
     1261 acttcgccgt cgtatcgggg ttgcgttgtc ccgcgaaccg tccgcaggga ttgcgcaaag
     1321 cgcggacacg gtaaataata ttgtaatttc aattggttac gtcacggtag acattcttgt
     1381 gacgcctggc tttccgtctt cctggcgaaa cacctgacgc ttcgtcatcc aatgcccttc
     1441 gcgcatctcg cgggcaccag cccatcggtt taccatggca tctgtggcgg tgatggacat
     1501 ggcgggtggt tgtaccatca ccaatctgcc tcgatttcat cacgacacta tcccgttgcc
     1561 cccacccaaa agccaagggg caggctgctt gatgaaaaga ggccacaaga acgaaccttt
     1621 ccctcgccat cctctcccgc tctcgatgca gtcgcggggc cgcagaaaac gcgggtggca
     1681 caaagacgtc gagcaggcgt tttttgcgga cgccgcaaca gatggctgcc cttggcagcg
     1741 gcacgaacgt cgagggcccg ctcggacgtc atgaaatcct tccactgcca tcgatttcgg
     1801 tagcatcggt cagggggttg gcgtgtcgcg atagaagcca tcgaaccatg gcttggatgc
     1861 atttaccgaa tagttcccag attttgttcc catgaaactg tttgttcttg cgcaacctac
     1921 gttcttccgt tagcttgcgc ggcccgttgg tcttccacaa ggagatcagc ccgtccccat
     1981 cgtctagagg cccaggacac agggttttca tccctggtac gggggttcga atccccctgg
     2041 ggacgccgct aggggcgcca tgaaaacgat atgcccttgc tgtgtgcgca tcgtctttcg
     2101 ccaacgcccg tgtagctcgc ggctggtttg caggcagtcc atccgaagtc cttggggata
     2161 ctcgcggcgc gcatcgtcct cgactcgccc tgggtggctc tgtccgttgc gctctgccct
     2221 ggttacgaaa cggtccacag acggtcgatg ggtctgtagt gcggaagaat cgaccgcacg
     2281 tcgacggcct agccgagcca tggtcgtcaa gcttacgcga ccgcaagtcg cgcgcctttg
     2341 ggctcgagtc gatgagaaga gtattgatgt aatatattga atttattgaa ttatgttgct
     2401 gtgcgggacg ggtggccctt gcggttcgat caccaggttt tgattatttc gtagatggca
     2461 aactgcgggc gaatgtccac ggggatgccg gaaccgagac tgtccagggc ttttttcatt
     2521 tccggcgaga cgtttgcata cttggcaaga aactgtttcg ccccttcata gtccccatcc
     2581 acctcgaggg tgagatagac ctcggcgagc tttcggacgg ctgctttgac ttttttgaag
     2641 tcgatgccga atcgctccgc ctggttgtca taggtgatgg cgccttgttc acgcaagaaa
     2701 ttgaaggagg ccaccgctcc tcgcccatgc gcctcgccca cgccaaatcg cacttgtcga
     2761 aataccgtgg ccaggtacgc gacatagatg ggcttgtcgt aggcgcgagg gagcagtcca
     2821 cgatcgatga ggtattgcgc gctgacgagg ccggcgatat cggccttggc ttcttcgatc
     2881 gccccatatt gttcgcgcaa catcttgttg acctcggctt tggaaccatc ggccaacgtg
     2941 atgatgccag gcccaaggcc gtgggcgact tcgtgcatca agatataaga aaacatggct
     3001 tcgaagtcga gcagctcccg ttggtccgcc accaacacgc gttcggtgat cgggatcacg
     3061 atgtggtcga acttgccgcg aagcacattc ttcatcatga cctttttcga gcctttctcc
     3121 tttcgcacgt gctcgtcgtt gggaagattg tacgccacgg tctgcacccc ggcttgaccg
     3181 gcattgcaaa gaagctgcac cacgtcgatt ggtgagcctt gatgtcgctc ggccatgcgt
     3241 gcgcgctgct tggtggtcat gggaagattg cgctcgagcg cgttcatctg cttgccgatc
     3301 gtttcgagtt tcctggactc ctcgacatcg cgaagagaaa cgaatgcctc gaaactcgcc
     3361 ttcaaaccca gtagttgatc cgcataggtt tcatacggac caatggtgac ctcgatgggc
     3421 cccttgacat ccatccaggc gcgatcactt tcgcggtact cattggatag aaacgcatcc
     3481 gcgcgttttt cgaggaaggc tcgaagcgtc ggctcgttcg tcaaagaagc cgctcgtcga
     3541 agcgcttggg ccgccggtcc gagcttgtcc gcataggctt tcgaataagg taaggcttcg
     3601 agcgccccgt cgtgacgccg aatcaccgta aagtatccca gaaaatcgtc tttctgatcg
     3661 ggatgggcct tcacgaacgc ttcgaactca tctttggtca tgtccgcggg atgaaaagcg
     3721 cctcctggca aacgagcttt cgtacccacg aagggctcgt cgtggttcaa cgtatcccac
     3781 ggtccagcca tcacgtcgaa gtacgccaac gccgtggggt gagagaggag ttgagatcga
     3841 atcgatggaa tctgcggatc gatctggtcc atgaataact cgtgcatcat gcgagatgcg
     3901 tcgacgagag ccgcaagcac cttcttgtca tcgctggaga tcgtggagtc atcgaagtcc
     3961 atgacgacgg tagcgaattt cgcgagccga gcatcgatgt ccggcgcgat ggtcaacgcg
     4021 ggtggttccg ggggcaacgg cggtgggggt tgttgttgtt tttcgggcat catgggcgca
     4081 ggaggaacgc tgggttgtgg agatgggccg ccacaagcgg ctatcacgag agaaagaacg
     4141 ggaagaaaac gtcgcatcgg gtttccttat gggttggaaa ggttgcgagg tagggacctt
     4201 cgcacggggc aaaggtcctc gatgatcacg ccagatcggc caggactttc ttgtaaaagt
     4261 cccagaactt caccacggtg gagattttta ctttttcacc gggggcatgc accccttgaa
     4321 gctccggacc aaacgagatc atatcggcgt cgctgccgat cttttcgccg atgatgccgc
     4381 actcgagccc cgcgtggatg gcagccactt tcggttcttt tccaaacaat tgcttgtaaa
     4441 cacgttggca gaccccaagc agtttcgagt ccatattcgg ctgccacgcc ggatacgcgc
     4501 ccacctccgt atatacaccg cccgccaagg ccaccacggc ttttgcctcg tccagcacac
     4561 cacggagcgc cgatgctaca ctcgatcgac tcgatgtcgt gatcgctatc ttgtcgccgt
     4621 cggtgctcgc gatggcaaga ttgctcgatg tttccaccaa acccgcgatg tcacggctca
     4681 tggcagatac tccatcgggt aaagccgtga gcatggccac cagccgtcgt gatgcctccg
     4741 cgctggccac cttcttcacc tctgccttat cagctcggat ggtaagcccg ccatcgatcg
     4801 tcttgaattc cgtaaggata tacgcttgct cctcggccgc gatcgtcttg gcagactcga
     4861 cgaaatgcga aggaatcgcc accaccgccg tagcttcccg aggaatcgca ttgtgcttgt
     4921 taccaccatc gatggattcg atgaatacat cgtgttggtc gagccagcga cccagcaccc
     4981 ggcaaagcac cttgatggag ttggctcggt tgtcgatgat gttcagaccg ctgtggcccc
     5041 ccttcaagcc ggtcacttcg agcttcatgc cgacgtagcc cgaaggaagc gaaacgcgag
     5101 gcagatcgaa ttggatttcc gaggtgcatc ctcccgcgca tccgacgaac aacacgccat
     5161 cttcctcgga atcgaggttg agcatgattt tgcccttcaa gatggaacca tccaggttcg
     5221 tggcgcccgt caaccccgtt tcctcatcaa tcgtaaacaa caattcgaga ggcccatgca
     5281 cgacatccgg atcgtcggcc gctgccatgg ccgccgctat accaatgccg ttgtcggccc
     5341 ccagagtcgt cttatccgcc accacccaat cgccgtccac gcggcaagca atggggtcct
     5401 tcgtaaaatc gaattcgaag tcgctgtctt tctcgggaac catgtccaag tggccttgga
     5461 taatgacgac gctcgcgttt tcgtgacctt gggtacccgg cacacgcacg caaacattgc
     5521 cgacttcatc ctccacccac tcgaattttc gacgttcagc ccacgctttg acgtgggtgc
     5581 gaatggcctc ctcatgccgt gaaggacgtg gaatctggcg aatttgatcg aaatgttgcc
     5641 acagatgctt gggttcgagg ttcgcaagcg gactggtcat aatgcctcct gaaaaccggc
     5701 gccgaccggt aagcctcgcc ggatgagatc gtgtggggtc aatcgcaccg taagacgcgc
     5761 gatgcaagcc tagaactccc acggcaatgt ccagccctgg taatggttcg atttttcaag
     5821 ccaatgactc gtacgaacgc ttcgcccctc acgcttggtc gttgccctcg tcatcgtctt
     5881 cgtcgtgacc gaaatcatgc ccttcatcac gcactcgccg ctgcacgatc gaggaacggc
     5941 gcccgtacgc gatggaatgc accgatttga cttcgcgcag gcgagtcaaa aggtcgagga
     6001 cctgttgttg ggtatcggcg agcaaggtga gttccacgac atcgagatcg ctgccccgcg
     6061 catggtcgaa ttgcagggaa cgaaggcgaa cgggagtctc ggcggtggcg aaatgaatgc
     6121 cttccaacgc gtcgacacca tggtctacgg taagtaaaat tctgtgttgt ttcgcgtact
     6181 tagcgaatat tttccgttcg atgggtttca ggcccgcttg tacgagaagc atgaacgccg
     6241 ttcctaccaa cgcggccacg tacaaaccgc ctcccgcggc tagtcccacg ccagccaccg
     6301 cccataccgt ggcagcggtt gtcaaacctc gcaccgtatt gtctcgaaag atgatgaccc
     6361 ccgcaccgag aaaaccgaca ccgctcacga cttgtgcggc gattcggctc gggtccagcg
     6421 aacccgagga gcccgcggga ggcagcggaa atccgtaggt ggatacgatc atcgccaatg
     6481 ccgcgcccac ggatacgaga gcatgggtgc gcaatcctgc cgcccgatcc cgcctttcac
     6541 gctcgaaacc gatggcaccg cccagaatcg tggccgccgc caaacgaagg gtcacctccc
     6601 aataggccaa ctcgccccaa tcggcgctcg tggcccacgt tgttgttcgt aaccaatcga
     6661 aaaaaaagtc cattgcgctg ataacatcct atttcacgat tccagcccaa gcgccaaggg
     6721 tttttggtga gaaggtacga tctttcccac ccttggattg gccattgctc gttgtccgag
     6781 cctatcgagc cgctttgcag caacgaaaac ccgtttgcgc gccggcatag gtctcgttat
     6841 gggaagaggt ggcggcgcgg cagcggtttc ggcccattag ccaccagcct cctcgcaaaa
     6901 tcgagcgatt cggcggtttg ttacccggtc gttgcgtcca ttcgtccaca ttgcctacga
     6961 ggttgaatac accgaaggga cttttgcatc ccggcaggga atcggcggca acccgctggt
     7021 cgaatagctc attgcctcgc acgaataagt tacttttatc gtgattgcag cgagcgccgt
     7081 cgcgcacata cccatacgga tagggccatg cctccggtcc ttcgcaagcg aattcccatt
     7141 cctgttcgaa gcacatcctt ttatccatct tgcggcataa gtattccgcc tcgggaaatg
     7201 caacgttcac aagcgggagc cgttcgcccg gagaagtaaa ttcgtagcgg tcgatgcaaa
     7261 accgcatctg gcgtcgcggc cctttgcata ccgtcttttc gaattgtccg caggctcgct
     7321 ccggcattcc cgcagggtcg atgaagccct ggcagacctg ttcgacgacg gcacaacgct
     7381 cgccttgcac caataccata tcgacggggc acggggcttt tctcgactca tagggcggag
     7441 gggaaacgtg gggcaaaaag ccaaaggcgt tgtgaaagga acggggctcg tcgcgacggg
     7501 tcgatcgggc ggggggttga acggcaggtg gattcacgga ttcggaagcg gggggggaga
     7561 cggcgtcagc gggggtcgta ggcggtgggg acggagccgt ctttgcggtt ttgcagcagc
     7621 gaaatccggt agtaaagtcg tgataatgct ctttatggtc gggggttaca taatgacacc
     7681 cctgcccgtt gaccaccgaa tccacataat gtcctcccat gagcagcggt cgggtcggcc
     7741 ccttggtcca ttcgagcagg ttgcccacca ggtcgaaaac gccctcgggg gttttgcact
     7801 cgggataagc ccctgttttc gcaagggtat ccggttgctg gttgagacgg gcgtcgttga
     7861 gcgtgacggc atcgtgccgg cgccgtcctg gaaataccag actcactgga tgaattcggg
     7921 ttctgtcgtt gcacgcgcgg gcgcggtgtt tctggccgta tggataggtg cgcttgggtt
     7981 tttccacgcc ctggcaagcg tcgagccact cctgggtcgt gcaaatccgt tttcccgcgg
     8041 cttcacaggc gcgcgtcgcc tgaaaaaggg agatatgcgc ttgcggcacg acatccggac
     8101 ggcttacggc tacgaccttt ttggtaccga caggcaagta agggctgatc ggtgagccgt
     8161 gttcatcgac caacgaagct tcccattggt cgacacagag ctgcggtccg acaagggcca
     8221 tgccttgagg gcagggctcg aaggctgaag gttcgggcgt ggtcgtggaa agggtcgggc
     8281 gaaccgaaga tacggcaggg ggcggcaccg aggcagcgac aggggttgct tgggcactgg
     8341 acgcctcgcc cacgatctcg gagccctgcg tcggctgctt acaggcgcaa acgcccaacc
     8401 ccatcgaaag gacggtcaag gttgcgatcg agcgcatggt tcccccgctg acgttcatga
     8461 aagccgtagg aatcgaaggc aaggaagcca gccaaatata gcgaaaccct ggcggataac
     8521 caagaatccg gtcggatttc gggtgcttag gcatgcacga ccaccacggt ggcggctccc
     8581 gtttccgtcg tgacggtcat gccatgccaa ccttccggaa tcgagggttc cgcccataag
     8641 aacagccatc gcgcatcgat gggggccacg ttgacgcatc ccaagctgac tttttctccc
     8701 caggcatcat gccaataggc ggcatgcatg gcgtaagcgc cgaaataggg catgacgaaa
     8761 ggcacgtcga agtgcatggt gctgttgacg gcgagggttg atgtccagta ttttccgaga
     8821 atcgagaaaa tgccaggtgg ggtggcggat tggagtacgt tggagccttt ttcgatcggt
     8881 tctccccggc cagtggcgac cagggtggcg aaaacgggac gatttccctc ataagcgatc
     8941 agccatcctt catgcacgct tacttcgacc caggttcgcc gtggtggagg cggcggcgct
     9001 ttggttccaa gggaccagcg cactttatcg tacaaacctg gtccctgggt ggggtccccc
     9061 catgggttga cgggcgacgc tttgatttcg acgatatcgc tggcgcgcgc ccagagatta
     9121 tctttggttt ccatgtagtt cgtgccgttt tgctccacgg tttgacccgt caatccgaac
     9181 gaagcatgct tcggccagtc tgctccacga ggaacgatcg ttccgtctgc ctgacgaagg
     9241 aattaattgt gtacgatctt tcacaaatcc aatcggcaag gtgtcatttt cctccaaatt
     9301 gcgcccttga aacgtggatg ggtggtaggg ggccaggcgg tcccgaggca cgaaaacacc
     9361 gtcgcccgtc accacccacg ctcgtccttc ggcatcgaaa acgtgggaat aactcatggc
     9421 ggagcccgcc ttcaccatcg gcacatgctc gtggaccctg ggcagggagt cgacaaaaga
     9481 agggatggcc tggccggaca gcgatacatc cacgtctcgc aacaaaaacg gtatttcccc
     9541 gacatgatcc cccgtacgaa gggctgtgac catcgcaaag tgcgagtcga actgaaactc
     9601 ccgctgcctt tgctgctcta ccgtgggcag cttttcgtag cgcggggctt gccgggattc
     9661 ggcatagtcg tgaagccaag gccggtcgcg actaggtgcg atatgttgga ccgccaccac
     9721 ggccggatcg gtggggtcca aagaaacacg acgatcggcg caaacgtagc ccctgggttc
     9781 gatggcataa aacgcatcgc agcccactcc ccgaaccggc tccggctccc gaagaaccac
     9841 gcggctgcca agtcccaaaa agccaatcca aggcgcttgc gtcgacggac gcgttcggat
     9901 ccacacatgc cgatgaagag cgtataatac ggggatattt cccgtctgct ccaggtcctc
     9961 ggcagacgcg gcagcaggca aatcctcctc cgcttcccct cccgcctgcc cgtgcggtat
    10021 cgctggcgtg ctcgacaaca aggattgcac gggagtgtca cggtcaggag cgcagccgaa
    10081 aaacatggta gcgctaaaaa acaggcgaga ataaaggatc tggcgggaca tgtcgtgcaa
    10141 ggtatccatc ttccttacct tggccaaaga aaccgatcga ccgccgcgac gatatccggc
    10201 accgatcttg gctagtcccg gagctgccag cggtctgccg taggtaatgc gtcattcctc
    10261 gccgcgcagt cccaaggctt tgcacttttt ataaaaatgg cttcgttcca attcgagacc
    10321 gcgggcggtg gcggccatct gcccgccgaa atgcgcaagg gcttcttgga caatcatgcg
    10381 ctcggcttcc tccgcgagca cccgaaacgg aactccgggc cggtacaatc cccccgccga
    10441 ggcctgtggt cctgccccaa ggcatacccg aacatcttcc cctccaatcc tgtcgtcgtg
    10501 agataaaatc accaatcttt ctataatgtt acgcaactcc cgcacgttgc ccggccagct
    10561 atgctggcaa agcgcctcca tgcctccgtc gtcgatactc gcttcgggtc ggtcattgtt
    10621 gcgcgccgcc agcgccaaaa agtggcgagc cagttccgga atatcctccc gtcgggcgcg
    10681 taacggggga atcacaaggg gaacgacgtt caatcgatcg tataaatcgg ggcggaatga
    10741 tccttgttgg caagcttcga cgagatcccg gttggtggcg gccaccacac gcacgtcgat
    10801 gggtaaggtt tcattgcccc cgaccctttc gatttcatgt tcttgcagca cacgcag
//
LOCUS       DAOSOR010000138        10856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6042_length_10856_cov_5.421350, whole genome shotgun sequence.
ACCESSION   DAOSOR010000138 DAOSOR010000000
VERSION     DAOSOR010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 10856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10856
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6042_length_10856_cov_5.421350"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..372)
                     /locus_tag="PK710_09535"
     CDS             complement(100..372)
                     /locus_tag="PK710_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10001.1"
                     /translation="MLDHVESAKGLRDIASEFSLNGQARHDALLGWCSAGVDRAIGVA
                     GVIDGWTGGQSEPWALLAHCIRKGKAMGGREKRRGGSAVSAAIIYS"
     gene            361..1122
                     /gene="ttcA"
                     /locus_tag="PK710_09540"
     CDS             361..1122
                     /gene="ttcA"
                     /locus_tag="PK710_09540"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007972.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HOT10002.1"
                     /translation="MIQHDDRILVALSGGKDSYTLFHLLDDLSRRSPVRFTLVPVHID
                     QGQPDHDPSPLIQFLHQRGFHLHVEREDTYSIVRSKIPEGKSFCSLCSRFRRAILYRL
                     SDELNCNKIALGHHRDDAIVTLLLNLVFSGQLKSMPPKLIADDGRHVVIRPLIYCSEP
                     SIVKLSSSYGFPILPCRVCGSRPDAQRKAIDDWLGDLEHRHPDVRANMLAALANVRPS
                     HLLDPDLWHALNLKVACEDTLDPHDTIAAERLIRT"
     gene            1119..1850
                     /locus_tag="PK710_09545"
     CDS             1119..1850
                     /locus_tag="PK710_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10003.1"
                     /translation="MTTETPAQEPTRKASAFLYRLIEASQVLLPGIYAWFITVVPLVA
                     FRAQSPWPLVTASLALVSLFVGASLLNRPTIAHAVGIWGFLLACLATWLLTPLYWLER
                     LDPWRAAAGSLGWILFALGWGTPWRLGHHPEDNPRASLSPKLEPRTRARRSSLVAVAI
                     GSLGALSTMMLAWRATAPERALFLHGAAFACAIAMLNASAAVALSLSKNRIPFSPRQR
                     ISYAFPWVMAISALVVVAIAWWLGS"
     gene            1941..3116
                     /locus_tag="PK710_09550"
     CDS             1941..3116
                     /locus_tag="PK710_09550"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT10004.1"
                     /translation="MIKLLKKFGYLPRLAVWELTLKCNLNCRHCGSRAGRKRADELTT
                     PEALKLCKDLAELKCRHLTLSGGEPLLRKDWPLICSTLVDLGVKTNMVTNGKSWNKDT
                     TKTALAVGLESAAFSIDGLEETHTYIRREPGHWKHVWSCIDHCKQMGLTASVITVVHR
                     KNLHELDELRQLLIDHGVDRWQVQLANPTGNMADHRDLCLEPEDVLVVVPKLAELCAK
                     KSRPKVYPAHNVGYYGEPEEHLRDTGGAIPFWVGCTAGCSVIGIESNGGIKGCLSLPS
                     AMNGEDRFLEGNIRDTPLREIWYRRGAFSYNRDFTTEQLSGYCAECDYAEICRGGCSW
                     TAFAYTGSRFENLYCYWRQLKEKEKREAASRKESSSNDNQPSTPADSPKRRIPVVQG"
     gene            complement(3133..3975)
                     /gene="proC"
                     /locus_tag="PK710_09555"
     CDS             complement(3133..3975)
                     /gene="proC"
                     /locus_tag="PK710_09555"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOT10005.1"
                     /translation="MSTYRVGFLGAGNMARALVKALLEAQRATPDQILASDINEERLE
                     AIAERFGIHTTLDNAQVVRFSDILFLSVKPQVVDKVLPTIALHLRPETLVVSIVAGVP
                     LRALEARLPQGSKVIRTMPNTPAMVLAGATALSAGEHVTAEEVRIAKDFFKTLGRTVV
                     LDETLLDAVTGLSGSGPAYVMLMIEALADGGVKVGLHRETALMLAAQTVFGSAKLLLE
                     TGEHPGRLKDMVTSPGGTAIAGLHTLESGGLRRTLMDAVENSTRRAAELGAEMEHKLT
                     QDNK"
     gene            complement(4051..5145)
                     /locus_tag="PK710_09560"
     CDS             complement(4051..5145)
                     /locus_tag="PK710_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10006.1"
                     /translation="MSSSLKPFLAQMLRVLSDDALFLLCSTHKADRDTWTEMTTRRSN
                     QYTKIVQGAHSDSEDESAWMVNMCVALAPVAPPMWLPMHELIDSLTLESGARGVRSLF
                     TSKPSEKQIERVRIVGSFAVQALSSVLDAGTPWTEDDQLLRRCVVAALGLPEDDANRL
                     CQQAPRSTEDLELPADFDAKLAKQVASGLWRAAFRDGLDPREDERVMQLCQRMGLTSE
                     DTEAARRQARDDVDSRKALGVAAVDAVRYVLSDDAGACVRLSKMVAKLALPPVHRAES
                     MAAIEHGGPIVLARRHELERKQREVCLTLTWVAALATNPEQTRLAEYLVRHDRVASDI
                     GSKGEGPAARAFATSFLQEQLASAVQAGGL"
     gene            complement(5158..6288)
                     /gene="pcnB"
                     /locus_tag="PK710_09565"
     CDS             complement(5158..6288)
                     /gene="pcnB"
                     /locus_tag="PK710_09565"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HOT10007.1"
                     /translation="MSAPPVHRVRIDIDESLLDPDALKVVRRLVRFGFEAYLVGGGVR
                     DLLLGCRPKDFDVATAARPEQVRRLFRNSHIIGRRFRLVHVVFGVGKVIEVATFRRCP
                     ETEDTRAETEAMLIRSDNAYGQAHEDALRRDLTINALLYDVQKHEVLDFVGGMPDIHQ
                     RVIRTIGDPRTRFLEDPVRMLRAIKFSARLNLGMVPELYDAMVLVRETLTVVAKPRLL
                     EEILKLLRSGASHRAFWLLWETGLLHVFLPEVAVLLDDASENVDGCERFWRMLTQVDH
                     RTAQRGAPFDDMTLLTILLLEPIVEALEGQRDRLSAAMEVARPIFQRLAVPRRMADGV
                     CRVAADLYAPRPRKSKRIAKTELYKAALDVHAIAQASRESEP"
     gene            6634..6891
                     /locus_tag="PK710_09570"
     CDS             6634..6891
                     /locus_tag="PK710_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HOT10008.1"
                     /translation="MPPFHELIGYAAAVLTTSSFVPQVAKVLRERQTQGISLTMYILF
                     TIGVALWVLYGLIIGSTPVFAANAVALLLAGTVLVMKLRLG"
     gene            complement(6901..8592)
                     /locus_tag="PK710_09575"
     CDS             complement(6901..8592)
                     /locus_tag="PK710_09575"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT10009.1"
                     /translation="MPIRICPKCQLEIDTEEPFCPRDGTRLLSRSVYDQIARETDPLV
                     GQRIAERYLVIRKLGEGGMGEVYLAEHEDIEKRLALKVLKHEYSTRADVVARFKQEAI
                     SASRIKHPNVVDVFDFGQLDDGRFYLAMEWLDGSDLADALATKGTLEPARGVAIAMQM
                     CRALAAAHAKGVVHRDLKPENIFLCVDEVGQEQVKIVDFGIAKLRDVTKEGEQAERRK
                     LTKTGMIFGTPEYMAPEQASGKNVDDKVDIYAMGVILFEMFAGSVPFSGDTFMAVLTA
                     HMTEAVPAIRDFNPSSPISEALEQAIYRALNKKPGLRHESMSAFAQALAQTPEAQTAG
                     VDRLPVIVGTGKKHNIINGLYTNGPTLMPADMAHDAKVPIDTQAGGNSKSKGWIAVGV
                     IGAAVLAGVGVFVLRLDREHEPVWEASTSTSVSLSESPSAPVIAVSSSSGAVVVEEAK
                     TIVLRIESNPSGAVIKKKDGNDLFQVCAAAPCEYPARLDEKVELVAEKASLRGTAAVF
                     ARTSQTIVIDLRATGSSRPSPKPTDTSRTNNCEFCVPKGDPDCIKVIRPCDRPPY"
     gene            complement(8676..9860)
                     /locus_tag="PK710_09580"
     CDS             complement(8676..9860)
                     /locus_tag="PK710_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10010.1"
                     /translation="MAAWRCLVAMCVVGCVDDVTTDVVVSPADAASVDAVQEADAALE
                     AADSDCTDDGLDEEEDAAIAPLVIGLDPIPADAQGKDEDSQQAEALLMALAAGVRGVV
                     VEANWKALEDDAWWARTEVLSSYLSKERKQLFLSIPVVEARQDGRPEWLRFYSWSSAT
                     VRERLRQLLDRVFGTLGSQLSYVSMGFEVDRYLEAYPKDAMAFSSCMAETLQYAMNHD
                     KRQDKTEVGVTWSTSAWLGQEAESAERQMLVGASPTVFLAHHGLDNEQGIEHWQQAVQ
                     AIGVAVDAVEAQGRKVVLHRVASTSSTLVGGSEAEQAAFVKGVFELVQAKRSHVAFVG
                     FGMLHDPSPDACLSFARAREPSATAGLYAFWCSAGLRTRQGAAKASFSVFMQEASRFL
                     NP"
     gene            complement(9905..10099)
                     /locus_tag="PK710_09585"
     CDS             complement(9905..10099)
                     /locus_tag="PK710_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOT10011.1"
                     /translation="MKTEIQIGGMSCQHCVKAVEQALGTVQGVQQVEVSVGRAVVITE
                     RAPDMNAIRHAIEEEGFQVI"
     gene            complement(10139..10369)
                     /locus_tag="PK710_09590"
     CDS             complement(10139..10369)
                     /locus_tag="PK710_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10012.1"
                     /translation="MEGEDEIATYSDGLELVTVTREGVDGMYFDAPAGQVVRFEVYWD
                     GRPEARFTYWAGDGAVHHGAPTNPIDLLPSEP"
     gene            10521..>10856
                     /locus_tag="PK710_09595"
     CDS             10521..>10856
                     /locus_tag="PK710_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10013.1"
                     /translation="MARSPALLSAVVRILCAEIFRLMRQLSQQRGVRHEATGIVAIFQ
                     YLSGSIKRDRVGNCTLQPASKRKSPDVPTPSIPIRSRWGSLSAINTGGDDGDDAGVSP
                     APQCRWDHGW"
BASE COUNT         2399 a         3683 c         2543 g         2231 t
ORIGIN      
        1 tccgaggtca atccgtgcag accattcgcc ccctcaatcg tgatttcctt gggaagtgcc
       61 aaatccctca tcggataaat aagtgataac tatttgatat tatgaataaa ttatcgccgc
      121 cgacacagcg ctcccccctc gcctcttttc cctccctccc atcgcttttc ccttcctgat
      181 gcaatgcgcc aacaacgccc acggctcgga ctgtcctccc gtccacccat cgatcacgcc
      241 cgccacccca atggcacggt cgacacctgc cgaacaccat cctaataacg cgtcatgccg
      301 cgcctgcccg ttgagagaaa actcgctcgc gatgtcgcga aggcccttcg cggattcgac
      361 atgatccaac atgacgaccg cattctcgtc gccttgtccg gtggcaaaga ctcctacacc
      421 ctctttcatt tgctcgatga tctaagccgt cgttcccccg ttcgcttcac cctcgtgccc
      481 gtgcatatcg accaaggaca acccgatcac gatccttccc ccttgatcca gttccttcac
      541 caaagaggct tccaccttca tgtcgaacgg gaagatacct actcgatcgt gcgctccaag
      601 atcccggaag ggaagagctt ttgctcactc tgttcccggt ttcgacgcgc gatcttatat
      661 cgactttcgg atgaattgaa ttgcaacaaa atcgcgctcg gacaccatcg tgatgatgcc
      721 atcgtcacgc tcctgttgaa cctcgtcttc tcaggtcaac tcaagtccat gcctcccaaa
      781 ctcattgccg acgatggacg gcatgtggtg attcgtccgt tgatctattg ttccgaaccc
      841 tccatcgtga aactttcgtc aagctatggc tttcccattt tgccttgccg cgtgtgtggc
      901 tctcgccccg atgcacaacg aaaggccatc gatgattggc ttggagatct cgaacatcgt
      961 catcccgacg tgcgagcgaa tatgctggcg gctctcgcca atgtgcgtcc ttcccacctt
     1021 ctcgatcctg acttatggca tgctctgaac ctgaaggtcg cttgtgaaga cacgctagac
     1081 ccacacgaca ccattgccgc tgagcggttg atacgaacgt gacgaccgaa acgccagccc
     1141 aggagcccac acgaaaagct tcagcttttc tctatcgcct catcgaggca agccaagtgc
     1201 tgcttcctgg aatctacgcc tggttcatca cggttgttcc gctcgttgcc tttcgcgcgc
     1261 aaagcccatg gcccctggtg acagcctcgc tggctctcgt ttcgttgttc gtcggcgcta
     1321 gcctgctcaa ccgtccgacc atcgctcatg ctgtcggaat ctggggcttc cttctcgctt
     1381 gccttgccac ttggttactc acgcccctgt attggctcga aagactcgat ccctggcgag
     1441 ctgccgcggg ttcgctaggt tggattttat ttgctctcgg atggggaacc ccttggcgcc
     1501 tcggccatca tcccgaagac aacccccgcg ccagcctctc ccccaaactc gaaccaagaa
     1561 cccgcgcccg tcgcagttcc ctcgtggccg ttgccatcgg cagtctcggc gccctcagca
     1621 ccatgatgtt ggcgtggcgc gccacggcac cagaacgcgc cctgttcctg catggcgccg
     1681 ctttcgcttg tgccatcgcc atgctcaatg cctcggcagc cgttgccctt tcgctaagca
     1741 aaaaccggat ccctttttcc ccgcgacaac gaatttccta cgctttcccc tgggtgatgg
     1801 ccatcagtgc cctcgttgtg gtggccatcg cttggtggct cggttcctga ccctgtcacc
     1861 cttctgaacg ccaaaatcgg gcaggcgttg gtcagtcgat ggctccgggg gtatgctgcg
     1921 tacacggtgg agaaccagat atgatcaaac tcctcaaaaa gttcggctat ctcccgcgcc
     1981 tcgctgtctg ggagcttacc ctcaagtgca acctgaattg tagacattgc ggctcacgag
     2041 ccggaaggaa acgcgccgac gagttgacta cgcccgaggc tttgaaactt tgcaaagacc
     2101 tcgcggaact gaaatgccga catctcacgc tgtccggagg tgaacccctt ttgcgcaaag
     2161 actggcccct catctgctcg accttggtgg atctcggcgt caaaaccaac atggtgacca
     2221 acggcaagtc ctggaacaaa gacaccacca aaacagctct cgccgtcgga ctcgaaagcg
     2281 ccgccttcag catcgatgga ctcgaagaaa cccataccta tatccgtcgt gaaccaggac
     2341 actggaaaca cgtctggagt tgcatcgacc attgcaaaca aatgggcctc accgcttcgg
     2401 tcatcactgt cgtgcatcga aaaaacctac atgaactcga tgagttacgg cagttactga
     2461 tcgaccatgg cgtcgatcgc tggcaagtgc aactcgccaa cccgaccggc aacatggccg
     2521 accatcgcga cctatgcctg gaaccggaag acgtgctggt cgtggtaccg aaactcgccg
     2581 aactctgcgc caaaaaaagt cgccccaaag tctatcccgc tcataacgtt ggctactacg
     2641 gcgaaccgga agagcacctt cgcgataccg gtggcgcaat tcccttctgg gtgggctgta
     2701 ccgctggatg ctccgtcatc ggtatcgaaa gcaatggcgg catcaaagga tgcttgtccc
     2761 ttccctctgc catgaatggg gaagaccgct ttctcgaagg aaatattcgc gacacacccc
     2821 tgcgcgaaat ctggtaccga cgaggcgcct tttcctacaa ccgagacttc accaccgaac
     2881 agctcagcgg ctattgcgcc gaatgcgatt acgcggagat ttgccgcggc ggatgctcct
     2941 ggaccgcctt cgcatacacc ggaagccgct tcgaaaacct gtattgctat tggcggcaac
     3001 tcaaagaaaa agaaaaaaga gaagccgcct cgcgcaaaga atcctcctcc aatgacaacc
     3061 aaccgtccac gcccgcggac tctccgaaac gacgtatccc cgtcgtgcag ggatgaatca
     3121 taaacccccg ttctacttgt tgtcctgcgt caacttatgc tccatctcgg ccccaagctc
     3181 cgcggcccga cgcgtcgagt tttctaccgc atccatgagg gtgcgacgaa gccctccgga
     3241 ctcgagcgtg tgaagaccag caatggccgt tccgcctggg ctcgtgacca tatccttcaa
     3301 ccggcccgga tgttcaccag tctcgagcaa tagcttcgcg gaaccgaaca ccgtctgggc
     3361 tgccaacatg agcgctgttt cacgatgtaa accaaccttg acaccaccat cggcaagcgc
     3421 ctcgatcatc aacatcacat acgccggacc acttcccgat aaccccgtca ccgcatcgag
     3481 caacgtttca tcaagcacga ccgttcggcc caaggttttg aaaaaatctt tggcaatccg
     3541 gacttcctcg gccgtgacgt gctcgccagc agacaacgcg gtagctcccg ccaacaccat
     3601 cgcaggtgtg ttgggcatcg ttcgaatgac cttcgatccc tgcggcaaac gtgcctcgag
     3661 agcacgcaac gggactcccg ccacaatcga tacgaccaac gtttcaggac gaaggtgaag
     3721 cgcaatcgtg ggaagtacct tgtccaccac ctgcggtttg acggacaaaa acaggatatc
     3781 cgaaaaacgc acgacctgcg cgttgtccaa cgtcgtgtgg atgccaaacc gctctgcgat
     3841 cgcttcgagc cgctcttcgt tgatgtcgct cgcaagaatt tgatccggtg tggctcgctg
     3901 cgcttccaac aacgccttga ccaacgctcg ggccatgttg cccgcaccca aaaaaccgac
     3961 acgatacgtg ctcatcgatg catctcctgg agcgcacgca tgacgcaatc ggattgtctt
     4021 gtccagggga ggttccaaca atcgttgtcg ttatagaccg cccgcttgta ccgccgacgc
     4081 tagctgctcc tgcaaaaagc tcgtggcaaa cgctcgcgcc gccggcccct ctcccttcga
     4141 tccaatgtca ctcgctaccc gatcgtgccg aaccaaatac tccgccaagc gcgtctgctc
     4201 agggttcgtc gccaacgccg ctacccacgt caacgtcaaa catacctcac gctgcttgcg
     4261 ctccaattca tgccgtcgtg ccaatacgat aggcccccca tgctcgatcg ctgccatcga
     4321 ttcggctcga tgaaccggtg gcaaagccag cttcgccacc attttcgata accgtacaca
     4381 cgcgcccgcg tcgtcggaca acacatatcg aacggcatcg accgccgcga cccccaatgc
     4441 cttgcgtgaa tccacgtcgt ctcgtgcctg acgacgcgct gcttccgtat cctcggaggt
     4501 caatcccatc cgttgacata actgcatgac tcgctcgtct tcacgtggat cgagaccatc
     4561 acggaatgcg gctcgccaca aaccacttgc cacctgcttg gcaagcttgg cgtcaaaatc
     4621 cgccggcaat tccaagtctt cagtactcct tggagcctgt tggcacagac gattcgcatc
     4681 gtcctccgga agcccaaggg ccgctaccac acaccttctc aacaattgat catcctcggt
     4741 ccacggtgtt ccggcatcga gtacggagga caacgcttga accgcaaacg aacccacgat
     4801 ccgaacgcgc tcgatctgct tttcactcgg cttcgacgta aacaacgatc gaacgccccg
     4861 cgccccagat tcgagcgtca atgaatcgat gagttcatgc atcggaagcc acatcggagg
     4921 agcgaccggc gcaagcgcca cacacatatt caccatccag gcagactcat cctcactgtc
     4981 cgaatgagcc ccttgcacga ttttcgtgta ctgattcgat cgcctcgtcg tcatctccgt
     5041 ccacgtgtca cgatccgctt tgtgagtcga acaaagcaaa aacagcgcat catcgctgag
     5101 cacacgtagc atctgagcaa gaaaaggctt gagagacgac gacatggttt ggctccttca
     5161 aggctcgctt tcacgagacg cctgcgcaat tgcatggacg tccagagccg ctttgtacag
     5221 ctctgtcttg gcaattcgtt tgctctttcg cggtcgcggc gcgtataggt ccgccgcaac
     5281 acgacatacc ccatcagcca tgcgacgagg aaccgccaac cgctggaata tcggtcttgc
     5341 aacctccatg gccgcggaca gtcgatccct ctgtccttcc aacgcctcca caatcggctc
     5401 gagcaacagt atcgtcagaa gcgtcatgtc atcgaaaggc gcaccacgct gcgccgtgcg
     5461 atgatccacc tgggtgagca tccgccaaaa ccgctcacaa ccgtccacgt tttccgaagc
     5521 gtcatcgagc aataccgcca cctcgggaag gaagacgtga agcaacccgg tttcccacaa
     5581 caaccaaaac gcacgatgag aggctccact gcgcaatagc ttcaggatct cctccaatag
     5641 ccgtggcttg gcgacgaccg tcagcgtttc gcgaaccaat accatcgcat cgtatagttc
     5701 aggtaccatc cccaggttca gacgcgcact gaacttgatc gcgcgcaaca tgcgaaccgg
     5761 gtcctccaaa aaacgagtgc gcggatcccc gatggtccgt atcactcgct ggtgaatgtc
     5821 cggcatccct cccacgaaat cgagcacttc atgcttctgc acgtcgtaca gcagcgcatt
     5881 gatcgtcaga tcacggcgaa gggcatcctc gtgcgcttgc ccatacgcat tgtcgctgcg
     5941 aatgagcatc gcctccgtct ccgcccgtgt gtcctccgtc tccggacacc gacgaaacgt
     6001 cgcgacttcg atgactttcc caacgccaaa cacaacgtga acgagacgaa acctgcgccc
     6061 aatgatgtga gaatttcgaa atagcctgcg cacttgctct ggacgcgctg ccgtggcgac
     6121 atcgaagtcc ttcggacgac acccgagcaa caaatccctt accccgccgc ctacgagata
     6181 cgcctcgaat ccaaaccgaa cgagacgacg gaccactttg agagcgtccg gatccaagag
     6241 cgattcgtct atgtcaatgc gaacacgatg aacgggcgga gcagacacat ccgataactc
     6301 cgattcggag atacctggta tgcgacgtcg agtctctgaa cgcgtgcgat aatgcaaacg
     6361 cgaactaccg ggaggaacag ccattgaaac agatacaatt gccgaacaac ataagggctt
     6421 ccgctactat gccctgtatc gacaagccat tggggttaat ggtcaacttg caatggaccc
     6481 taggatagca ccttgtcgca ccttgcgtaa tgaccgaaaa cgtgactgcc cgtattcttt
     6541 tctcagccat cgccttttct gaccaccccg cgcaaaccgc cgaagcccac cccttgcctc
     6601 tcgcctcgac cgtcgcctat gacccccccc atcttgccac cgttccatga actcatcggc
     6661 tacgccgccg ccgtgctgac cacgagcagc ttcgtccccc aagtcgccaa ggttctgcgc
     6721 gaacggcaaa cccaaggcat ctccctgacc atgtatatcc tgttcaccat cggtgtggcc
     6781 ctttgggttc tgtacggcct gatcatcgga agtacccccg tattcgccgc caatgccgtg
     6841 gccctgctgc tcgccggaac cgtcctcgtc atgaaactgc gcctcggcta accatagccg
     6901 tcaatacggc ggacgatcac agggacggat gaccttgatg caatccggat ctcccttggg
     6961 cacgcagaat tcacagttgt tcgtccgaga agtatcggtg ggcttgggtg atggtcgcga
     7021 tgaaccggtg gcacgaagat caatcacgat ggtctgcgaa gtccttgcaa atacggccgc
     7081 cgttccacgc aaggaagcct tttccgccac caactcaacc ttctcatcca aacgcgccgg
     7141 atactcacaa ggagccgctg cacatacctg aaataaatcg ttcccatcct ttttcttgat
     7201 caccgccccc gatggattgc tttcgatccg aagcacaatc gtttttgcct cttccaccac
     7261 caccgcccca ctcgacgatg acaccgcaat gacaggtgcc gatggggatt ccgacaatga
     7321 cacagacgtg ctcgtactcg cctcccacac cggctcatgc tctctgtcca acctcaagac
     7381 gaacactccc acaccagcga gcacggccgc tccaatcacg cccacggcta tccaaccctt
     7441 tgacttcgag ttccctccag cctgtgtgtc gatcggcacc ttcgcatcat gcgccatatc
     7501 cgcaggcatg agggttggcc catttgtgta taacccattg ataatattgt gttttttgcc
     7561 ggttccgacg atcacgggga gccggtcgac tcccgccgtt tgggcctcgg gggtttgtgc
     7621 gagcgcctgc gcaaacgcac tcatcgattc atggcgcaag ccaggtttct tgttcaacgc
     7681 acgataaatc gcctgctcga gcgcttcgga aatgggcgaa ctcggattga aatcccggat
     7741 ggcagggacc gcttcggtca tgtgagcagt caataccgcc atgaacgtgt ctcccgaaaa
     7801 cggtacgctg cccgcgaaca tttcgaagag aatcacgccc atcgcgtaga tgtccacctt
     7861 gtcatccacg ttttttcccg acgcttgttc gggcgccatg tactcgggcg tgccaaaaat
     7921 cattcctgtt ttcgtaagct tccttcgctc cgcttgctca ccttccttgg tcacgtcgcg
     7981 aagcttcgcg atgccgaagt ccacgatctt gacctgctcc tgcccaacct catcgacaca
     8041 caaaaaaata ttttcaggct tcaagtcccg atgaacgacc cctttcgcat gagccgccgc
     8101 caacgcacgg cacatctgca tcgcgatcgc tacaccccgc gcaggctcga gcgtcccctt
     8161 cgtcgccaag gcatccgcaa gatcactgcc atcgagccat tccatcgcaa gataaaagcg
     8221 gccatcgtcg agctgaccaa aatcgaatac atccaccaca ttcggatgct tgatgcgtga
     8281 tgcgctgata gcctcttgct tgaatcgcgc gaccacatcc gcacgggtcg aatactcgtg
     8341 cttgagcacc ttgagcgcca aacgcttctc gatatcctcg tgctctgcca gatatacttc
     8401 ccccatccct ccttccccca acttgcgaat cacaaggtaa cgttccgcta tccgttggcc
     8461 aaccaaaggg tcggtctcgc gcgcaatctg atcgtatacg ctacgagaca acaacctggt
     8521 cccatcacga ggacagaacg gctcctccgt atcaatttcg agctgacact tcggacatat
     8581 ccggatcggc atgggaaaaa aacaccgcgg ctcgtcgcaa acacagccat cctaaccgtc
     8641 tttgctcacc cataccacga acaaaccacc cgccttcatg gattcaaaaa tcgtgaggcc
     8701 tcctgcatga aaaccgaaaa agaagccttg gccgcccctt gtctcgttcg taaacccgca
     8761 ctgcaccaaa acgcatatag ccctgccgtg gccgatggct cccgcgcacg agcaaacgaa
     8821 agacatgcgt ccggcgacgg atcgtgaagc atcccaaagc ccacgaatgc cacgtgagat
     8881 cgcttcgctt gaaccaactc gaataccccc ttcacaaaag ccgcctgctc cgcctcactt
     8941 ccccccacaa gcgtggaact cgtgctcgcc acccgatgca acacaacctt gcgaccttgc
     9001 gcctccactg cgtccacggc aacgcctatc gcttgcaccg cctgctgcca atgctcaatc
     9061 ccctgctcgt tatccaaccc gtgatgcgcc aaaaataccg tgggcgacgc ccccaccaac
     9121 atctgccgct cggcagattc cgcctcttgc cccagccacg ccgaggtgga ccacgttacc
     9181 ccaacctcgg tcttgtcctg ccgcttgtca tggttcatcg catattgcaa cgtttccgcc
     9241 atgcaactcg aaaacgccat cgcgtctttg ggatacgcct ccaaataacg atccacctcg
     9301 aatcccatgc tcacatacga aagctgtgag cccagagtgc caaatacccg atccaacaat
     9361 tgccgaagcc gttcacgcac cgtggcggat gaccaactgt aaaaacgcaa ccactccggg
     9421 cgaccgtcct gccgcgcttc cacgacggga atcgaaagaa aaagttgctt acgttctttc
     9481 gaaagatacg acgaaagcac ttcggtccgc gcccaccagg catcgtcttc caacgctttc
     9541 caattcgctt ccaccaccac gccgcgaaca ccagccgcca aggccatcaa caaggcttcc
     9601 gcttgttgcg agtcctcatc cttgccttgc gcgtccgcag gaattggatc aagcccaatc
     9661 accaatggcg ctatcgcagc gtcttcctcc tcatccagcc cgtcgtccgt acaatccgaa
     9721 tccgctgcct ccagcgcggc atccgcctcc tgcaccgcat ccacgctagc cgcgtccgca
     9781 ggcgaaacca ccacatccgt ggtcacgtcg tcgacgcagc ccaccacgca catcgctacc
     9841 aagcaacgcc atgctgccac ggaacgaacc ataccacgag ccggacccga ggttcggcaa
     9901 ggactcaaat cacctggaat ccctcttctt caatggcatg gcgtatcgcg ttcatatccg
     9961 gcgcccgttc cgtgatgacc accgcccgac ccacactcac ctccacctgt tgcactccct
    10021 gcacggttcc aagcgcctgc tccaccgcct tgacacaatg ctgacaactc attccaccaa
    10081 tctgtatctc tgttttcatc cgaccaacct aatgcacgaa aacaaggttg gacagcggtc
    10141 acggctcgct tggcaacaag tcgatcggat tggtcggagc cccatgatga accgcaccat
    10201 cccccgccca ataggtgaat cgagcctccg gacgaccatc ccaatacacc tcgaaccgca
    10261 ccacctgccc cgccggcgca tcaaagtaca tcccatcgac cccctctctc gtcaccgtca
    10321 ccaactccaa cccgtccgaa tacgtcgcga tctcgtcttc accctccatt tcgagcgctt
    10381 cccccgtgtt tcgcggcgtg tgcccgtcgt gcgcagcaag gcgcgcgagt cagaccgcgg
    10441 caaacctggt ggaccgagtc ctgccccgcg ttgccttatg acaaggggtg ctgagcgtac
    10501 actacgaact tcgcctaccg atggcacgca gcccggcttt gctcagcgcg gtggtgcgca
    10561 tcctgtgcgc ggagatcttc cgtttgatgc gacagctcag ccagcaacgg ggagtccggc
    10621 acgaagccac gggaatcgtc gcgattttcc aatatttatc tggatccatt aagcgagatc
    10681 gcgtaggtaa ctgcacttta caaccagctt ccaaacgaaa atcaccggac gtgcccacac
    10741 catcgattcc aatccggtcc aggtggggct cgttgagcgc aatcaacact ggcggtgacg
    10801 atggagacga tgcgggcgtc tcgcccgcgc cccagtgtcg ctgggaccac ggctgg
//
LOCUS       DAOSOR010000139        10837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6058_length_10837_cov_4.052217, whole genome shotgun sequence.
ACCESSION   DAOSOR010000139 DAOSOR010000000
VERSION     DAOSOR010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 10837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10837
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6058_length_10837_cov_4.052217"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1149)
                     /locus_tag="PK710_09600"
     CDS             complement(145..1149)
                     /locus_tag="PK710_09600"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT10014.1"
                     /translation="MKIGLTLGKFSPLHKGHQFLIETALGEVDKLYLLLYDSPETTTI
                     PLSVRAGWVRRMYPQVQVVEAWDGPSDAGYTPEVIRIQDTFVLSHFHDKNITHFYSSE
                     RYGEHVSKALRAVNRVVDRARRKVPISGTQIRENPYAARQYLSDVVYRDLIVNVVLMG
                     APSTGKTVLAEYLAQRFHTVWMPEYGREYWDAHQIDRRLTQAQLVEIAEGHLEREERQ
                     LLRANRYLFTDTNALTTRLFSYHYHGTADPTLEALADRCASRYDLYCVCVPDFPYVVC
                     PDRSGEGSRLILQKQCLADLHCRKIPYLLVAGSVAERADQIARALEGLQKYTNPWRLG
                     "
     gene            complement(1146..1754)
                     /gene="pnuC"
                     /locus_tag="PK710_09605"
     CDS             complement(1146..1754)
                     /gene="pnuC"
                     /locus_tag="PK710_09605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOT10015.1"
                     /translation="MSVLELVAVICGFLCVLLTIRENIWCWPAGLVQVTLYVFIFFEA
                     KLYSDVLLHIIYIFMNLYGWYFWLHGGRERQKAKVTGLGLPLFVASAALSAAGTLALG
                     YMMESRTDASYPYADAFTTAFSLMAQWLMSRKKYESWDFWISVDVVAIYIYGAKGLYA
                     TALLYVAFLVMAVLGRRKWYHSMRQSEEIRRVESRPEWEPAS"
     gene            complement(1757..3103)
                     /locus_tag="PK710_09610"
     CDS             complement(1757..3103)
                     /locus_tag="PK710_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10016.1"
                     /translation="MNPVEGLTDRQRFPLIDDLRLLHRLRQDESAPRFNFASGDRLDG
                     AGLEKVHEYRRSLDTQPFWLPGQLPAWMTTFLDWCQRQVPAYRDIGSCFQAIPTLRRP
                     QIAARPWQYVAAEARLEDMLVFCTSGTTEAPMDVLADPVSQACWLPQLESILERDRIR
                     FDREGQKVAICLVCDQKDTLTYASLSTWLGGAGVLKINLNPEEWSKPEDRCVYLERYN
                     PEIITGDPFSLWSMARVAPRIRPKAMVSSAMTLHQGARQLLQQQFGCPVYDIYSLTEC
                     RMIAVSTEPARYRLIRPELYVEILSPDSDIAVPEGAVGEITVTGGNNPFLPLVRYRTG
                     DFASLKYVDGHPCLCDLEGRSPVIFLRQNREFLNPIDISRALSKVALAGFTLHQREDL
                     SLEFSAWGAELEARSLETTLKQLFGDAIPLQMTLSVESPPSPQKVKYTSEINEIEI"
     gene            complement(3103..3678)
                     /locus_tag="PK710_09615"
     CDS             complement(3103..3678)
                     /locus_tag="PK710_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10017.1"
                     /translation="MQKAVTISELIHHIKQCPSEFLDMPARGENDDGVHTLALVNDIL
                     RYICHCPTTPCLLTVEGKADKTAEWRLWQVCCWALSHPVFFEAGPAAQWIPLFSGRLD
                     EVSTLVETEKWVTDEERAEELARILLSHAAIIPEGEDATIAADRLDAIDTVKRMKVIE
                     QTRQAELRTQEIRRQMAARAAREAANAYGRE"
     gene            complement(3663..5483)
                     /locus_tag="PK710_09620"
     CDS             complement(3663..5483)
                     /locus_tag="PK710_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10018.1"
                     /translation="MTRETSEQPPEIAEVLARWQETWPTALQLWNPYVSLREPQWCLT
                     TAEAREAGLQDSFAMIRLRGLAVVVDLETVRKLRVENYALQVLAHEIGHHLLVPANLY
                     DYAGLMMRVRLALAGIEDRAPLVANLYADLLINDRLQRTMGQDMASLYRHLREKGIGE
                     TRIFLWYMRIYELLWSLPRGFLSCDKSEPVLDADASLGASVLRSFARDWLDGAGRFAM
                     LAYPYLIEDALHQQALREFAHLSDITSAGVGAELPAGMAEIPSSVFDGIVDPREEVLQ
                     NAPNHQEADSPGRAKPGSLTAIIPGSDASTGPQQRYMQPGIYVDLMRQIHPDVDEDRL
                     IGRYYRDVVTPHLVPFPVEALGELSGDVLPEGTDPWEPGDPIEDLDWFESALLPPIVI
                     PGVTTRRRVYAEDIAEPEKEKPYHLYVGIDCSGSMHNPRRTFSWPVCAGALVSLSALR
                     VGARVMACLSGEPGSFMETRGFIKEENEVLRVLTSYLGTGYAFGIPRLETEHLQNAKE
                     KTHLLIVTDDDIFSMLAAPRSDGQTNYDIAEQALRRAGGGGTLVLHARAEWRPQEAER
                     LRQMGWVIHYVTDENALFDFAKAFANRFYRSQGNPTCKRP"
     gene            complement(5473..6420)
                     /locus_tag="PK710_09625"
     CDS             complement(5473..6420)
                     /locus_tag="PK710_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10019.1"
                     /translation="MEQLTFSPLIEHLREHAAELNQLRKACIPDASRMDTAMAVRWFH
                     EVIEPVFVAVCQYDPARAPQVFRILYIDMLRVFAAGRAQADPLVIQCRRLLAHNPKLT
                     ASEPQRILELLDHALRHIVSHSPKAAMPWLELMEKVLGKVADIDLMILAARFAAWRCG
                     MAHLRHLLDPWPALPVALREIIFAGEPIGEQEVQTGWTKGAPVYAWTIGAFKGFQGKF
                     RRPPRVTLHDGLVVATDGEVQAVVFADRFGVVLHDAMRLAPPSFPKKPPPHSSLRADI
                     ARIIGSYSDCTSWVLHDSALYLTTACSHAIFLFGAFHDS"
     gene            complement(6408..7997)
                     /locus_tag="PK710_09630"
     CDS             complement(6408..7997)
                     /locus_tag="PK710_09630"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT10020.1"
                     /translation="MSHLSDFARAMLGKIPASPTNPRSVDHQPGFKIEDLFSDDPAAG
                     TIEVGGLDEKLRQAYFWVTNTAIISPFYDIEYHEEAPRTFQFGDSKFELTLPTGQSYS
                     SFVLIPLLNLAVRGRCLIVGGPGRGKTATAVLMGLLAGYSQLEVKRAIQHGQPQMTIA
                     DLLGNPLPSDMMKAENIGDIRISWRKWLSMRVKIIDEYNRIPTRTQSALLTVLADNYA
                     EILDQVYECPEAAWYLTANDDGGGGTYQVIEALRDRIDVVIRAFHFNTRFIADLQQRI
                     ELSFRPEEVIPREIIFSEEELTRMNRAIRNVPIDTSVRRALEFFCRHFEYFEPSSPRL
                     EYMTKDTAKLSGRDFGLLLKEITGKDLIKDLGAQTHNGLSVRKLMTIIMFSKALAFFR
                     GSSFVGMEDMRQMIPFVLHDTLVPHLEAPFFDQPGHDSYRIDRISWLRKLFDLSNAEY
                     YRLNLQNDDPVGVFEEEFRAGLDGLSLKEVEKRLNAIERYLGTLAKTGKMFGHLADDI
                     LTLKYLHQRYTNYRRWLKWNS"
     gene            8267..10123
                     /locus_tag="PK710_09635"
     CDS             8267..10123
                     /locus_tag="PK710_09635"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOT10021.1"
                     /translation="MRYSRELQVAIETASIAGRAMRTAFHATGHTLTEHGLDASLEAM
                     IREPLGAAFPSYGQLGEELPQADRLATDEQAHVWVIDPLDGSTHFEKCRRGAALSIAL
                     LRAGEPVLGVVFSPLYPNDKGDMIAWCEGQGLIRNGVPVHRRPLASALSSGHTVLTAP
                     GAERDVKGNAEIVSPARFRGMPSIAYRLALLAVGEAEASYSINGPVSWDYAGGHALLR
                     GVGGDLFDADGKPVVYGHDGRGGCGGICVGGTSTVANELLERLRQRRLGGHRSSSRKT
                     LDLRGRTIEDAGQLSRAQGCLLGQLAGDSLGSLVEFQSSERIKASYPHGIRELQDGGT
                     WGTLPGQPTDDSELALALARSLIEVGDYEGDAVARAYATWYSSSPFDMGSTTSMAFSA
                     AARATSEAALAAQNAANKQSQANGALMRISPLAIFGCSLPKEILVKLARYDASLSHPH
                     KVCQDVNAVYVVAIQHALKSGDPGEKVYRFVRAWAKTQDLDNAVMDWLRAAETSAPSD
                     YQSQMGWCKIAFQNAFYQLLHASCLAEGVEDTVLRGGDTDTNACIAGALMGAVYGIEA
                     VPFPWVDRVLTCRPMAGFRGVRRPRPVTYWPTDALELAEGLLDLSRKKASLC"
BASE COUNT         2447 a         3061 c         3030 g         2299 t
ORIGIN      
        1 ctatcccgcc atcaccccac cgatgctccc ggccctcgct tcgtcgtttt ctttcgccct
       61 cgcacggctt gcccgacccg gtgcctcccc acccccgtga gatggatccg taccgcccgc
      121 cgtgaaccat gtgggctcgt caaactaccc gagccgccag gggttcgtgt atttctgcaa
      181 cccttcgagg gcgcgggcga tctggtcggc gcgctcggcc acgctgccag ctaccagcaa
      241 atacggaatt ttccggcaat gcaagtccgc aagacactgt ttttgcagaa tgagacgact
      301 cccctctccg gagcggtccg gacaaaccac ataaggaaag tcgggaacgc aaacgcaata
      361 aagatcgtag cggctcgcgc atcggtcggc gagcgcctcg agcgttggat cggcggttcc
      421 gtggtaatga tacgaaaaca gacgcgtcgt cagagcgttg gtgtccgtaa atagatagcg
      481 attggcccgt agcaactgtc gttcttcccg ctcgagatga ccttctgcga tttccaccaa
      541 ttgtgcttgg gtcaagcgac ggtctatttg gtgggcatcc cagtattctc gcccgtattc
      601 cggcatccaa acggtatgga aacgttgggc aagatattcg gccaacaccg tttttcccgt
      661 ggaaggcgca cccatcagca ccacattgac gatgaggtct cgatagacca catccgatag
      721 atattgccgc gcggcataag gattttcacg aatttgcgtg cctgaaatcg gcactttccg
      781 acgcgcccta tcgaccacac gattgaccgc tcgcagggct ttgctgacgt gttccccata
      841 gcgctcgctg gagtaaaagt gagtaatgtt tttatcgtga aaatgcgaaa gaacaaaggt
      901 atcctgaata cgaattacct cgggggtata tccggcatcg gaaggtccat cccaggcttc
      961 caccacctgc acttgcggat acatacgccg cacccagccc gcccgaaccg atagcggaat
     1021 cgttgtcgtt tccggactgt cataaagtag taggtaaagc ttgtccactt cgcccagagc
     1081 ggtctcaatc aagaattgat gccccttatg caagggggaa aacttgccca gggtcagccc
     1141 gattttcatg atgccggctc ccattccggg cgcgattcga cacgccggat ttcctcagac
     1201 tgtcgcatcg aatgatacca cttgcggcgt ccaaggacgg ccatcaccaa aaacgcaaca
     1261 taaagcagcg cagtcgcata cagccctttt gcaccatata tataaatggc caccacatcc
     1321 acgctgatcc aaaaatccca tgattcatac ttttttcggg acatgagcca ttgcgccatc
     1381 agactgaacg cggtggtgaa agcatccgca tagggataag aagcatccgt acgactttcc
     1441 atcatatagc caagcgcaag tgtgccagcc gcggacaacg ccgcagaagc cacaaaaagt
     1501 ggcagcccta gtccggtaac cttggctttc tgacgctcgc ggcccccatg aagccagaaa
     1561 taccagccat aaagattcat gaaaatatag atgatatgca aaagaacatc ggaataaagc
     1621 ttggcctcga aaaaaatgaa gacatagagc gtgacctgaa cgaggcccgc cggccaacac
     1681 cagatgtttt cccgaatggt caacaagacg caaagaaagc cacaaatcac ggcaacgagt
     1741 tcgaggaccg acatggttat atttctatct cattgatttc actagtatat tttacttttt
     1801 gggggcttgg gggcgattcg acggaaaggg tcatttgcag cggaatggca tcaccaaaaa
     1861 gctgcttcag ggtcgtttcc aaggacctgg cctcgagttc cgcaccccag gcggaaaatt
     1921 ccagggataa atcctcacgc tgatgcagag taaagcccgc cagcgcaacc ttgctcaacg
     1981 cgcgtgagat atcgatggga ttgaggaatt cccgattctg acgcaaaaaa atgacggggg
     2041 agcggccttc gagatcgcaa agacatgggt gtccatccac atatttcagc gaagcaaaat
     2101 caccggttcg ataccgcacg agcggcaaaa agggattgtt cccccctgtc accgtgattt
     2161 cgccgaccgc gccctcgggc acggcaatat cgctgtctgg cgaaaggatc tccacataga
     2221 gttcggggcg aatcaaacga tacctagccg gctcggtgga taccgcaatc atacggcatt
     2281 ccgtaaggga ataaatgtca taaaccgggc aaccaaattg ttgctgcaaa agctgcctgg
     2341 ccccctggtg caacgtcatc gccgacgaaa ccatcgcttt gggtctgata cgaggcgcta
     2401 cacgagccat gctccacagc gaaaacggat cgcccgtgat gatttcaggg ttgtaccgtt
     2461 cgagatagac acaacgatcc tcgggcttgc tccattcttc gggattgagg ttgattttga
     2521 gaacccccgc cccgccgagc cacgtcgaga gcgaagcgta cgtgagcgta tccttttgat
     2581 cgcataccag acaaatcgcc accttctgcc cctcccggtc gaaacgaatc cgatcccgtt
     2641 caagaatgga ttcgagttgc ggaagccaac acgcctgcga taccggatct gccaggacgt
     2701 ccatgggcgc ctccgtcgtt ccggaggtac aaaaaaccag catgtcctcc agacgtgctt
     2761 ccgccgccac gtattgccaa ggacgggcag cgatctgcgg tcgccgcaag gtgggaatgg
     2821 cctgaaagca agagccaata tccctatacg cgggcacctg tcgttgacac caatccagaa
     2881 acgtcgtcat ccacgccggc agttgtcctg gcagccaaaa tggctgcgtg tccagcgacc
     2941 gacgatactc gtgtaccttc tcgagtccgg cgccatcgag tcggtccccc gaggcaaaat
     3001 tgaagcgtgg ggcgctttcg tcctgccgca gacgatgcag cagtcgcaaa tcgtcgatca
     3061 gcgggaatct ctgtcgatcc gtaaggccct cgacggggtt catcattccc tcccgtaggc
     3121 attggcggct tcccgggctg cccgcgccgc catctgacgc ctgatttctt gcgttcgcag
     3181 ctctgcttga cgagtctgct cgatgacctt cattcgcttg accgtatcga tggcatccag
     3241 acggtcggct gcgatggtcg catcttcccc ctccggaatg attgccgcat gggagagaag
     3301 aattcgggcc aattcctccg cgcgctcttc gtccgtcacc cacttttccg tctccaccaa
     3361 cgtcgaaacc tcgtcgagcc tgccggaaaa tagaggtatc cattgggcgg cggggcctgc
     3421 ctcgaaaaac acgggatggg acaaagccca acagcagact tgccataaac gccattccgc
     3481 agtcttgtcg gctttgccct ccaccgtcag caggcagggc gtcgttggac aatggcatat
     3541 ataacgcagg atatcgttga ccaacgcaag cgtatgaact ccgtcgtcat tttcgccgcg
     3601 ggcgggcatg tcgagaaatt ccgacgggca ctgcttgata tgatgaatca attcagaaat
     3661 ggtcacggcc ttttgcatgt tgggtttcct tgtgagcgat agaaacgatt cgcaaaggct
     3721 tttgcaaaat caaatagggc gttttcatcg gtgacataat gaatgaccca tcccatctgc
     3781 cgcaagcgtt cggcttcttg gggacgccac tcggcccgtg cgtgcaagac cagggttcct
     3841 ccgccccccg cccgccgtag agcctgttcg gcaatatcgt aattggtttg accgtccgat
     3901 cgtggcgctg caagcatcga aaaaatatcg tcgtcggtca cgatcagcaa atgcgttttt
     3961 tctttggcgt tttggagatg ctcggtctcc aatcgaggaa tcccgaaggc gtagcctgta
     4021 ccgagatagg atgtcaatac gcgcagcact tcgttttctt ccttgatgaa tccccgggtt
     4081 tccatgaacg agcccggttc ccccgacagg caagccatca cccgcgctcc cacccgcaag
     4141 gcggacaacg aaaccaatgc cccagcacaa actggccagg agaaggtgcg acgggggtta
     4201 tgcatggatc cggaacaatc gatacccaca taaagatgat aaggtttctc tttttccggc
     4261 tccgcgatat cttctgcgta cacgcgacgg cgggtcgtga ctccgggaat cacgattggg
     4321 ggaagtagcg cggattcgaa ccagtccagg tcttcgatcg ggtcgccggg ctcccaggga
     4381 tcggttccct ctggcaagac atcccccgaa agctcgccca aggcttcgac gggaaacggc
     4441 acgagatggg gggtgaccac atcgcgataa taacgtccaa tcaaccgatc ttcgtcgacg
     4501 tctggatgaa tctgccgcat caagtccaca tagatgccag gctgcatata acgctgttgt
     4561 ggcccggtcg aggcatccga gcctgggatt atcgcggtca aagaaccggg cttggcgcga
     4621 ccgggcgagt ctgcctcctg gtgattcggc gcattttgga ggacttcctc ccggggatcg
     4681 acgataccat cgaatacgga tgacgggatt tctgccatgc ccgcgggcaa ttcggcgcca
     4741 actcccgcgc tcgtgatgtc cgaaagatgt gcaaattcgc gaagggcctg ctggtgtagg
     4801 gcatcttcga tgagataggg ataagcgagc atggcaaacc gaccggctcc atcgagccaa
     4861 tctcgcgcga agcttcgtag gacggaggct cccaacgagg catccgcatc caataccggt
     4921 tcactcttgt cgcacgacaa aaagccgcgg ggcaggctcc agagaagctc gtaaatccgc
     4981 atataccaaa ggaaaattcg tgtttcaccg ataccctttt cccgcaaatg ccgatacagc
     5041 gatgccatgt cctggcccat ggtgcgctgc aacctgtcat tgatgagaag gtcggcatag
     5101 aggttggcga ccagcggagc ccggtcttcg atacccgcca aggccaaccg cacccgcatc
     5161 atcaagcccg cataatcgta tagattggcc ggcacgagaa ggtgatgacc aatttcatgc
     5221 gccaatacct ggagtgcata gttttcgaca cgcagctttc gtacggtttc gagatcgacg
     5281 acgaccgcaa ggccccgaag acggatcatc gcaaaactat cctgcaaacc tgcttctcgc
     5341 gcctcggcgg tcgtaagaca ccattgcggt tcccgcagac tcacataagg gttccagagc
     5401 tgcaacgccg tcggccaggt ttcttgccaa cgggcaagaa cctccgcgat ctctggcggt
     5461 tgttcggacg tttcacgagt catgaaaggc tccgaacagg aaaatggcat gggaacacgc
     5521 cgtggtcaga tacaacgcag agtcatgcaa cacccacgag gtgcaatcgc tataactgcc
     5581 aataattctt gcaatatccg ctcgtaggga cgagtgtggc ggcggttttt tcggaaagga
     5641 aggtggggca agtctcattg catcatgcag caccacccca aatcgatcgg caaagacaac
     5701 cgcttgaacc tcgccatccg tggcgacgac aagaccatcg tgcaacgtga cgcgaggggg
     5761 tcttcggaat ttcccttgga aacccttgaa ggcgccaatc gtccacgcgt acaccggcgc
     5821 tcctttcgtc cagcccgtct gcacttcctg ttcgccgatt ggctcgccgg caaagatgat
     5881 ttctcgaagc gccacgggca gggcgggcca ggggtccaac aggtgccgca aatgcgccat
     5941 gccacaccgc caggccgcga atctagcggc aaggatcata aggtcgatat ccgctacctt
     6001 cccaaggacc ttttccatca actctagcca cggcatggcg gctttggggg aatgcgagac
     6061 gatatgccgc agggcatgat ccaacaattc gaggattcgt tgcggttcag aggcagtcaa
     6121 tttcgggtta tgagcgagca aacgacggca ttgaatcacc agcgggtcgg cctgggcgcg
     6181 tccagcggcg aacacgcgca acatatcgat gtaaagaatt cggaaaacct ggggagcccg
     6241 ggccgggtca tactgacata cggcgacaaa aaccggttcg atgacttcgt ggaaccagcg
     6301 tacggccatc gccgtgtcca tgcggctcgc gtcgggaata caagcctttc gcagttgatt
     6361 gagttccgcc gcatgctccc gaaggtgttc gatcaatggg ctgaacgtca actgttccat
     6421 ttcaaccatc tccgataatt cgtataacgc tgatgcaggt atttgagcgt gagaatatca
     6481 tcggcaaggt ggccgaacat tttgcctgtt ttcgccaatg tccccagata tctttcgatg
     6541 gcgttcaagc gtttttcgac ctctttcaac gacaaaccat cgaggccagc cctgaattcc
     6601 tcttcgaaaa cacccacagg atcgtcgttt tgcaaattga gccgatagta ttccgcattg
     6661 gatagatcga atagctttcg cagccaactg atgcggtcga ttcggtagga gtcgtggccg
     6721 ggctggtcga aaaacggcgc ttcgagatgg ggaaccaacg tatcgtgaag aacgaagggg
     6781 atcatctgac gcatgtcttc catgcctacg aaagacgagc ctcgaaagaa agccagggct
     6841 ttggaaaaca tgatgatggt cattagtttt cggacggaaa ggccattatg ggtttgcgcg
     6901 cccaggtctt tgatcaggtc ttttccggtg atttctttca atagcaaacc aaaatcgcgc
     6961 cccgacagtt tggcggtatc tttggtcata tattccaggc gtggggaaga tggctcgaag
     7021 tattcgaaat gacggcagaa aaactcgagc gcacgccgta cggaggtgtc gatgggtacg
     7081 tttcgaatcg cgcgattcat ccgcgtcaac tcttcttcgc tgaaaatgat ctccctggga
     7141 atgacctcct cgggccggaa ggacagttcg atacgctgct gcaagtcagc gatgaatcgg
     7201 gtgttgaagt gaaatgcccg aatgaccaca tcgatgcggt cgcgcaaggc ctcgatgacc
     7261 tgatacgtac cgcccccgcc atcgtcgttc gccgtcagat accacgccgc ctcggggcat
     7321 tcatagacct ggtcgagaat ctccgcatag ttgtcggcca agactgtcag caaagccgat
     7381 tgggtccggg tgggaatccg gttgtattca tcgatgattt ttacgcgcat cgacagccac
     7441 ttacgccaag aaatgcgaat atcgccgata ttttcggctt tcatcatatc cgagggcaac
     7501 ggattcccca gcaggtccgc aatcgtcatt tgtggctgtc cgtgttgaat cgcccttttt
     7561 acctcgagct gggagtagcc cgccagaaga cccatcaaga ccgcggtggc cgttttcccc
     7621 cgtccaggcc cgcccacaat cagacaacgc ccccgcaccg ccaaattcaa caaaggaatc
     7681 agtacgaaac tggaatagct ttgaccggtc ggaagggtca gttcgaattt gctgtcgcca
     7741 aactgaaagg tgcgcggggc ctcctcatga tattcaatat cataaaatgg gctgatgata
     7801 gcggtattgg tcacccaaaa atacgcctga cgcagttttt cgtcgagacc tccaacctcg
     7861 atcgtccctg ccgccggatc gtcggaaaac aggtcctcaa tcttgaatcc tggctgatgg
     7921 tcaaccgaac gcgggttggt aggagacgcc gggatcttac ccagcatcgc cctggcgaaa
     7981 tcggatagat ggctcatctc tgcttctctc atgggagttg acgatggtag cctggacgat
     8041 catgacgttg tggctgaaac gatggtggat ggaataagta ccatgttttg gggggttgcc
     8101 tagaaccaca atctcacgct cgttaaatat tttcttcgaa gaaggaaaaa cagaataacc
     8161 aagcaaaata ccgctcgacg atggtccacc actccctcgt tttcgaggat atcgacggta
     8221 ttcattttga tttacggggg gttcggagct gtagtatccg tgatctatgc gttattcacg
     8281 ggagttgcag gttgcgatcg agaccgccag catcgcggga cgagccatgc gaaccgcatt
     8341 tcatgctacc ggccacacgc tgacagagca cggactcgat gcttccctgg aggccatgat
     8401 ccgcgaaccg ctgggggcag ctttcccttc ttatggccaa ctcggtgagg agttgccgca
     8461 agccgatcgt ctggctaccg acgaacaagc gcatgtctgg gtcatcgatc cgctcgatgg
     8521 cagcacccat ttcgagaaat gtcggcgggg agcggccctt tccatcgccc ttttgcgggc
     8581 cggcgagcct gtcctcgggg tcgtgttcag ccctctctat cccaatgata agggggatat
     8641 gatcgcgtgg tgcgaagggc aagggcttat tcgcaacggc gttcccgttc accgacgtcc
     8701 cctggcatcc gcgctctcga gcggccatac cgtgctgaca gcgccaggag ccgagcgtga
     8761 cgtgaaagga aatgcagaaa tcgtcagtcc cgcgcgtttt cgggggatgc catcgatcgc
     8821 gtatcggctc gcgttgctcg cggtgggaga agcggaagcc agctattcga tcaacgggcc
     8881 ggtgtcttgg gattacgcgg gggggcatgc gctgttgcgt ggagttggcg gcgatttatt
     8941 cgacgccgat ggcaaaccgg tcgtgtatgg gcacgatggc cggggcggct gcggtggcat
     9001 ctgcgtgggg gggacatcga cggtggcgaa cgagttgctg gaaaggctac gtcagagaag
     9061 gctggggggc catcgctcat caagccgaaa aacgctggat ttgcgcggtc ggacaatcga
     9121 ggacgcgggc caactatcga gagcgcaagg ttgtttgctc gggcaactcg cgggagatag
     9181 cctcggttcc ctcgtcgaat tccagtcttc cgagcggatc aaggcatcgt atccccatgg
     9241 aatccgcgaa ctccaggacg gcggcacctg gggcacattg ccaggacaac ccacggatga
     9301 cagcgaactt gcgctggccc ttgcgcgcag cctcatcgaa gtgggagatt atgagggtga
     9361 tgcggtagcc cgtgcgtacg ccacgtggta ttcgtcttcc ccgttcgata tggggtccac
     9421 cacgtcgatg gcgttttccg cggcggctcg ggcgacatcg gaggcggcct tggcggctca
     9481 aaacgccgcg aacaagcaga gtcaggccaa cggcgcgctc atgcgcatct ctcccctcgc
     9541 catcttcggc tgctccctac ccaaggaaat acttgtaaaa ttagctagat atgatgcttc
     9601 cctgtcgcat ccgcacaagg tgtgtcagga cgtcaacgcg gtatacgtgg ttgccattca
     9661 gcatgcgctg aaaagcggag atccgggcga aaaagtatat cgtttcgtgc gcgcgtgggc
     9721 gaagacacaa gatctcgaca acgcggtcat ggattggctt cgggccgcgg agacgtcggc
     9781 gccatccgat tatcagtcgc agatgggttg gtgcaaaatt gcctttcaaa acgctttcta
     9841 tcagttgttg catgccagtt gcctggcgga gggcgtggag gatacagtcc tgcgaggggg
     9901 agatacggac accaatgcct gtattgcagg ggcgttgatg ggggcggtgt atggaatcga
     9961 agccgtgcca tttccgtggg tggatcgcgt gctcacgtgt cgtcccatgg cgggattccg
    10021 aggcgtgcga cggccaagac ccgtgacgta ctggcccacg gatgcgctgg agttggcaga
    10081 ggggttgttg gacctgtcga gaaaaaaggc atccctgtgc taagactctt gctgtctgtg
    10141 atgcgtgaga gaacctccgg cagcatcgca tcccatccgg gctacgccaa accctgaaag
    10201 atcccctcat tatttcctga attctaaagg gatacgagct gggattcttg ggtgttgatt
    10261 ttggttcggc ccttgccctc cttggagcac tctcaacagt tgccccgggc tactatacat
    10321 tggactggcg ttctgcggtg gacgataagg gattaccgga accggtattg tgtgccgtgt
    10381 gccgtgcgcc gtgaccgtga ccgtgaccgg tttgcgtggg ccgtgaccgg gaccgtgatc
    10441 gtgaaggaaa gactcgggaa aaatgagggg atggctcagc gccaacccct tatgctaact
    10501 ctatgttttt gatgatgccg caaaagcagc gcccctcgaa tcgaccttcc tccgcgctcc
    10561 catcattcca atcgcccctt tttttgggac agaatgtgca tgcgtcctgc cgcgccctgg
    10621 tgatgccccc cggaggttct tgccttactt gcgtgaagaa ccggggtatt tcaatgctgg
    10681 tgccatcgat ggggggagta acagcgggat ggattggcag gcctggacgc cgcttcggag
    10741 gcggagcgag cggaaagctg gctcttcggt ggaaacgaaa ccgtagcgag tcgctgtcgc
    10801 atccgcttgc caccctcgac acccatcatg aaaaccg
//
LOCUS       DAOSOR010000140        10764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6111_length_10764_cov_4.311700, whole genome shotgun sequence.
ACCESSION   DAOSOR010000140 DAOSOR010000000
VERSION     DAOSOR010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 10764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10764
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6111_length_10764_cov_4.311700"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1148
                     /locus_tag="PK710_09640"
     CDS             117..1148
                     /locus_tag="PK710_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10022.1"
                     /translation="MVSRLNTDDTGLHPLTEWLVRRLTGPRGRQDWNRLVEAAWRHTS
                     TIPLRTLMPTETLRDVVLAQLRPDRLPDFLRPITRSLLPRLLELHRNDDAPLGRWVPD
                     TARGALERLASRPGLIHEDWIRALFRQRVAEAVMADALYRGIRDFSTIMPRLMLSLMP
                     TQRFSRLGGATIIGKRIVEELEKKLEPEIKTFLEGGTKKALSRAADFAVEHKDDEAAK
                     AFRRNIVQFVLSKSPSFHVQGVSEDMLADLEPIVTMITKHMATREETHRLAIHAIEEI
                     DRVYGDMTLAEALRAVGYDSEPDHQAWASLTWPVLVECMKAPEIRVWLEELVRDFLEF
                     FETEYRPSS"
     gene            1216..2073
                     /locus_tag="PK710_09645"
     CDS             1216..2073
                     /locus_tag="PK710_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10023.1"
                     /translation="MNDVIRSTCLSKRWPFHVFAAALAFVAPAALAQPTPIETSSSAA
                     AQPWSASSQATVGQPVLIHVIVALCSNEYTYCGSKAKGDPTDLDGNLYWGATFGARRL
                     FDSSYSPWTRISANPASGQVLEHLIYQRPSEAFGTVMVSLEAWNGTHTDEAIDRFFSI
                     ASGGGQVTVQGRNVPIHAVGYVGLNRLLDGHNLPTASGGQAIPSFVIARASERTFGPA
                     LRQAGSFPLVTVHGTVAPEGYTILAAARAMADNYSPRGIRHRMIEEYARYQRTSQRAA
                     AATFPWPHP"
     gene            complement(2144..3352)
                     /locus_tag="PK710_09650"
     CDS             complement(2144..3352)
                     /locus_tag="PK710_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10024.1"
                     /translation="MKLGLSYRLCLWVGVGLLIAACSNEDNIADSPDAGTGGAAGTGG
                     VAGSGGGVAGTGGEAVGGSSGSGGQQPDGGGCENACPGEGSTLCMGNRNIACKTSVSG
                     CLAWQAGDYCAQGQTCNADATSCIVPSSSCSADSECGCGCACVSGECRCTGAIPPSCD
                     TDADCGPACYGLACVSGACVQRTDVTCIDPCPYPGATRCMGNAEQTCELKTPGCLQWG
                     APSACPQDHVCNSSGTQCIAHTDQCSANDDCGCGCGCVAGECKCTGAIPPSCNSDADC
                     GPACSGLVCVGNECVQAGGCVDTCEKEGATRCVGTEILTCIQGASGCLLWGNGTSCPA
                     GQVCNSSANQCVPDSNSCSSDGDCGCMCFCHSGQCYCTGAIPPTCDSDADCGPACAGF
                     VCMAGKCVQL"
     gene            complement(3426..4115)
                     /locus_tag="PK710_09655"
     CDS             complement(3426..4115)
                     /locus_tag="PK710_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10025.1"
                     /translation="MNGPSRLRDTGSDEVRALLRHASPPRPIDAKAFSRGERHIARLA
                     AIPVGLGLVFWLKGIALGAGLGVVVVAGVVAVETTQTTTRVPAASVEPSPEPSVTKAP
                     AAPVLPSSQAPTEPPPDDSAVLPKASPVVSTKQPQDAGVDSLAQEALLIEQARGFVGS
                     DPARALSLTQEYRRRFPRGQLGLESEIVAMESLVQLGRRAEACARAAAVLQRAPGIYA
                     DRIRNLCPPEP"
     gene            complement(4112..4684)
                     /locus_tag="PK710_09660"
     CDS             complement(4112..4684)
                     /locus_tag="PK710_09660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT10026.1"
                     /translation="MDEVLKTEVGLFGVGDRPDVVTVYRQHADFVWRTLQRMGVEEAN
                     LDDVSQEVFVVVHRKLLTFDGTSRMTTWLYGICLRVASSWRKRAWRKREQMMAAPPEP
                     CSHEACFGEEYMQRQEAQRLLRDVLDRMDIDKRAVFVMYEVEELSTMEIASVLGVPMG
                     TVHSRLHAARKQFELVLSRLQKDKKRGGGA"
     gene            complement(4723..5784)
                     /locus_tag="PK710_09665"
     CDS             complement(4723..5784)
                     /locus_tag="PK710_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10027.1"
                     /translation="MASGFPKAFSTVVIAMGFVGTGLFGQMTSSRAATVEPVRLAWAR
                     GQGADFCASPAEIEDLVAKRLSTAPFSNLARRSIEAIVEHTEHGWRVRIQVREGSNLV
                     GTRELTSDTPDCEAVQSAAVLAIALVIDPNAKLSADPVQPPIVVSSEAPPPAPPPPPP
                     PTTVTSAVPAQPITPKPPPPSQPCEVVPQGQASLSMRGAVAAGLVPDASPAVELVGKM
                     PLGSSWSWLAQSLVVAESQPDDERFSFGLAAFGLGACYHEGESITVGVCASGWLGSTW
                     AVVHALTPTHPGGRAYMAISVGPMLRMNLAGPVDVELGADLFVPLKRRAFTVTGWDEP
                     VWRQPTVGGLGWGGLGLHF"
     gene            complement(5949..6728)
                     /gene="xth"
                     /locus_tag="PK710_09670"
     CDS             complement(5949..6728)
                     /gene="xth"
                     /locus_tag="PK710_09670"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOT10028.1"
                     /translation="MTSPLTLATWNVNGVRARLERVVDWLSQHPVDVLCMQESKAPDD
                     QFPASAFESLGYRVALHGQKSFNGVALISRFEMQDIYRGLQGDAQARLIGATIEQVRV
                     FSAYFPNGQSLDSDKYVYKLEWMRKLQALLREEIGKYPNIALCGDFNVAPEPRDTYDP
                     TVWEGNILCSERERNALADLRSLGLFDAFRALHPDEIAFTWWDYRALGFPKNHGLRID
                     HAYVTHPLLARVESVRIDREARKGKQPSDHAPLVMTLGVSS"
     gene            complement(6725..7720)
                     /locus_tag="PK710_09675"
     CDS             complement(6725..7720)
                     /locus_tag="PK710_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT10029.1"
                     /translation="MEILRIEGTDIDVSRVALGTWAIGGWLWGGSDEQLSIRTIHKAL
                     DHGITLIDTAPVYGFGQAETIVGKALASSSHRARARIATKLGLAWKDDKVRRDASRER
                     VEREVDDSLRRLGVDHLDIEQVHWPDPAVPAQETAQALLRLQQQGKIRAIGVSNYSPE
                     QMSAFLDYAPLHTSQPPYNLFEREIERDVLPFCRNHRITTLMYGALCRGLLSGRIRVD
                     TRFGEGDIRNVDPKFKPSRRQHYLAAVDELDRFAQDRGKTLIQLAVRWVLDQPGSPIA
                     LWGARKPEQLDPLDGVWGWRLSAQELADIDQIVVRHISEPVGPGFMAPPLHETRS"
     gene            complement(7730..8125)
                     /locus_tag="PK710_09680"
     CDS             complement(7730..8125)
                     /locus_tag="PK710_09680"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOT10030.1"
                     /translation="MRSLISVEGGPRYNDSAGRTCPMGGFALRQRFSATMTTSDDIVV
                     LPIDGVLDLHTFQPRDVKNLVPDYLEECRLRGILEVRIIHGKGTGTLRKTVHSILDRL
                     TEVVDYRLGGIDSGSWGATLVTLRPLEKK"
     gene            complement(8113..9003)
                     /locus_tag="PK710_09685"
     CDS             complement(8113..9003)
                     /locus_tag="PK710_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10031.1"
                     /translation="MRTAVIKAWGRVLSLLGLVSMILMGCGADPCGSADECKKWGQCT
                     LSHGVCVAAQDSDCESSQVCKQHGACVSIDGVCEARKDYDCKLTDACRLHGKCEARDG
                     VCVATSNRVCREKLACAEQGLCIAGEGGVCVAASDMDCARANICKNEGKCSAKEGKCV
                     AVFDDDCRRSPLCRESGKCAAGDYGRCVASSTMDCRASEICRKRGLCTESHGTCEAQD
                     NDCKDTAICKQRGMCTAVHGSCRINSDEDCAEAVSCRSKGLCSERGGRCMAGSDGDCR
                     KSLLCEKNGWCRAEKGRCVR"
     gene            complement(9000..9482)
                     /locus_tag="PK710_09690"
     CDS             complement(9000..9482)
                     /locus_tag="PK710_09690"
                     /inference="COORDINATES: protein motif:HMM:NF018095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOT10032.1"
                     /translation="MKPLFLSIFDRTRPRLWIALSVAGTLLSGTAWAEILAVVVDKAN
                     FREGPSTQSAVLYTADKYFAVEVVEKKDDWIKTRDFEGDVAWIAAHLVEKKSCVVVRV
                     PSALVRENPTKDSPVVFKSDRGEGMMVMRRQGSWLHVVNPDGAKGWVHRDVVWSGADE
                     "
     gene            9681..>10764
                     /locus_tag="PK710_09695"
     CDS             9681..>10764
                     /locus_tag="PK710_09695"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT10033.1"
                     /translation="MPPNDHPDDRLTTLAGHSFSSQYVPNVDYRLGPQVGQGAMAVAF
                     YALRTAPEGYCPVVIKMLRPSFVRQFGQAASLTVQKETVALGRLNERTPSTPFVVRLI
                     DTGQLGISDRFGPLQLPWLAVEYVHGGPEGTTLTQRVMKSVQDTSFAFDMKRAANAID
                     CLCAGLAAVHEVGVIHRDMKPSNVLCCGTGDDEILKVADFGVARPQGLAGTFFHDVIG
                     TLGYVAPEAFGSRGSECGPWTDVFGLAGIVFFLLTRHKYLRIKTPADMLTVMGSPERQ
                     SILESPMLSPELRRREYACRAIDRVLAQSTALDPNDRPASVEAFAAMIQPWLQPEAGS
                     HRPSPVSRSGLVPTQERPDDAPPWTWI"
BASE COUNT         2340 a         3492 c         2762 g         2170 t
ORIGIN      
        1 cgtgccctca atcgaaaata atattattat ttcactatgt tataagctgt gcgtcccatg
       61 ggaacccaca tctcctctcc aaccctcgaa gccaaccgcc aaccaagaat gaagatatgg
      121 tgagccgctt aaatacagac gacaccggtt tgcatccttt gactgaatgg ctcgtgcgcc
      181 gtctgaccgg tcctcgaggc cgacaggatt ggaaccgttt ggtcgaagcg gcttggcggc
      241 atacctcgac catccccttg cgaaccctga tgccgacaga gacccttcgc gatgtagtgc
      301 tcgctcagct tcgccccgat cggttgccgg attttttgcg tcccatcacg cgaagtcttt
      361 tacccagatt gctcgaactg catcgaaacg atgatgcccc tttgggccga tgggttcccg
      421 acacggcacg cggcgccctc gagcgcttgg cttcccggcc cggactcatc catgaagact
      481 ggattcgcgc gctgttccgc caacgcgtgg cagaagccgt catggcggat gccctgtacc
      541 gcggcattcg ggatttctcg accatcatgc cgcgactgat gctcagcctc atgccgaccc
      601 aacggttttc gagattgggg ggtgccacga tcatcggcaa acgaatcgtc gaggaattgg
      661 agaaaaaact cgaacccgaa atcaaaacgt ttctcgaagg aggaaccaaa aaagctctat
      721 cccgagcggc tgacttcgcc gtggaacaca aagatgatga agccgccaaa gcgtttcgtc
      781 gcaacatcgt gcaattcgtt ctgtccaagt ccccttcctt ccacgtgcaa ggcgtctcgg
      841 aggacatgct cgcggacctc gaaccgatcg tcacgatgat cacgaaacac atggcgactc
      901 gagaagaaac ccatcggttg gcaatccacg ccatcgagga gatagaccga gtgtatgggg
      961 atatgacgct ggccgaagcg ctgcgagcgg tggggtacga cagcgaaccg gaccatcaag
     1021 cctgggcttc cttgacctgg cctgtgctcg tggagtgcat gaaagcaccc gagattcgcg
     1081 tatggctcga ggagttggtt cgagattttt tggagttctt cgaaaccgaa taccgaccat
     1141 catcctagac gactgcccga gcgatccccc gtcgaatgag ccacgtagtt gccttgttgc
     1201 ccgaagtaag tgaaaatgaa cgacgtgatt cgctcgacct gcctttccaa gcgttggcct
     1261 ttccacgttt tcgccgcggc actcgctttc gtggcacccg cggcccttgc gcaacccacg
     1321 ccaatcgaaa cgagcagcag tgcagcagcc cagccctgga gtgccagttc ccaggccact
     1381 gtcggccaac ccgtactcat ccacgtcatc gtggctcttt gctccaacga atacacctat
     1441 tgcggaagca aggccaaagg tgaccccacc gacctcgacg gcaatctgta ttggggcgca
     1501 accttcgggg cccgacgcct tttcgacagt tcttatagcc cgtggacacg gatctccgcc
     1561 aatcctgcca gcggacaagt gctcgaacac ctgatttatc aacgacccag tgaagcgttc
     1621 ggcaccgtga tggtctcgct cgaagcctgg aatggaaccc atacggatga agccatcgat
     1681 cggtttttca gtatcgcgtc ggggggagga caggtcaccg tgcaagggcg caatgtaccc
     1741 attcacgctg ttggctatgt gggactgaac cgtttgctcg atggtcataa cctgccgact
     1801 gcttccggtg gacaagccat tccatccttc gtcatcgccc gcgcctcgga acgcaccttt
     1861 ggcccggcgc ttcgtcaagc cggatctttc cccctcgtta ccgtccacgg aacggttgct
     1921 ccggaagggt atacgattct cgcggccgcg cgtgccatgg cggacaacta ctcgccgcga
     1981 ggcatccgtc atcgaatgat cgaggaatac gctcgatacc agaggacctc gcagcgagcc
     2041 gctgccgcca cctttccgtg gcctcatcca taaaacgatt atatttcgct atgttacgcc
     2101 agactcaccg atcggcgagt ccagcgttga caggacgagc ctttcagagc tgcacgcatt
     2161 ttccagccat gcaaacgaat ccagcacagg caggaccgca atccgcgtcg gaatcgcaag
     2221 tcggtggaat ggcacccgtg caatagcact ggccggaatg acaaaagcac atgcatccgc
     2281 agtcgccatc cgacgagcag gagttggagt ccgggacaca ttggttggcg ctgctgttac
     2341 aaacctggcc ggctgggcaa gaggtgccgt tgccccacag caggcagccc gatgctcctt
     2401 ggatgcaagt caggatctcg gttcccacgc agcgggtcgc accttctttt tcgcaagtat
     2461 cgacacaccc gcctgcttgc acgcattcgt tgcccacgca aaccagtcca ctgcaagcag
     2521 ggccgcaatc cgcatcggaa ttgcaactcg gaggaatcgc tcccgtgcat ttgcattcac
     2581 cggccacaca accgcagcca caaccgcaat catcgttcgc agagcattgg tcggtgtggg
     2641 caatgcattg ggttcccgag gaattacaaa catggtcttg ggggcaggcg ctcggcgcac
     2701 cccactgcag gcagcccggc gttttgagtt cacaagtttg ttcagcattg cccatgcaac
     2761 gggtggcacc cggataaggg catgggtcga tgcacgtcac atcggttcgt tgcacacacg
     2821 cgccggaaac acaggcaagc ccataacaag ccggtccgca atcggcatcc gtatcgcaac
     2881 ttggcgggat cgcgcccgtg catctgcatt ccccggaaac acaggcgcag ccgcatccac
     2941 attccgaatc agccgagcaa gaggaagaag gcacgatgca cgaggtagca tccgcgttgc
     3001 acgtttgccc ctgcgcgcag tagtcccccg cttgccacgc aagacacccg gatactgatg
     3061 tcttgcatgc aatattccga ttgcccatgc atagcgtgga tccctcaccg ggacatgcgt
     3121 tctcacatcc tccaccatcg ggttgttgtc ctcccgatcc cgacgatccg ccaacggctt
     3181 ctccacccgt tcctgccacc ccgccgccgg aacctgcgac accccccgtt cctgccgctc
     3241 cacccgtccc ggcatccggg gaatccgcaa tattgtcttc gttactacaa gccgcgatca
     3301 acaaccccac acccacccac aaacacaatc gataggataa tccaagtttc atgatttcga
     3361 cctccttatg gtccatgaat ggctagacgt cgtcgatggc ttcaaaaaaa aaattgcttt
     3421 tctgttcaag gctccggggg gcagagattt cgtatacgat ccgcatagat accgggggcc
     3481 cgttgcaaca cggcggcggc tcgggcgcag gcttccgcac gacgaccgag ttgtaccaac
     3541 gactccatcg cgacgatttc gctctcgagc ccaagctgcc cccgaggaaa cctgcgtcgg
     3601 tattcttgcg tgagcgaaag cgcacgcgcc ggatcacttc ccacaaagcc gcgcgcttgt
     3661 tcgatgagca gtgcttcttg cgcaagcgag tccacccccg cgtcctgtgg ctgtttggtg
     3721 gacacgaccg gacttgcctt gggcaagacc gcggaatcat cagggggtgg ttcggtgggc
     3781 gcctggctcg aaggcaaaac aggagcggcc ggagctttcg tgacggaagg ctccgggctt
     3841 ggctcgacag aagccgcagg aacccgagtc gtcgtctgcg tagtttctac agcgaccaca
     3901 ccggcaacca cgactacgcc caatcccgcg ccaagcgcga tgcctttgag ccagaacacc
     3961 aacccaagac cgacaggaat cgcggcaagc cgggcaatgt ggcgctcacc gcgagaaaac
     4021 gctttcgcgt cgatgggtct gggaggcgaa gcgtgccgta acaacgcccg cacctcgtca
     4081 gaacccgtat ctcgcagacg agagggtccg ttcatgcgcc acctccacgt ttcttatctt
     4141 tctgcaacct cgacaacacc aattcaaatt gttttcgcgc cgcgtgaagc ctggaatgca
     4201 ccgtccccat cggtacgcca agcacgctcg caatctccat cgtcgacagc tcttcgacct
     4261 catacatcac gaataccgct cgtttgtcga tatccatgcg gtcgagcaca tcccgcaaca
     4321 atcgttgcgc ctcttgacgt tgcatgtact cttccccgaa acaggcttcg tgagagcagg
     4381 gctcaggagg agccgccatc atctgttctc gctttcgcca tgcccgtttg cgccaggatg
     4441 acgcgactcg caggcaaatc ccataaagcc atgtcgtcat ccgcgaagtc ccatcgaacg
     4501 tgagcagctt tcgatgaacg accacgaata cctcctggga tacgtcgtcc aagttcgctt
     4561 cttccactcc catccgctgc agcgtgcgcc atacgaagtc cgcgtgttgc cgatacaccg
     4621 tcaccacatc gggccgatcc cccacgccga acaatccgac ttcggttttc agaacttcgt
     4681 ccacgtcaga aggtgaatgt ccccatgcct ctcgcccctt cattaaaaat gaagcccaag
     4741 gcccccccac cccaaccctc ccacggtcgg ctgtcgccac acaggttcat cccatcctgt
     4801 caccgtgaac gctctacgct tcagcggcac gaataagtcg gctcccagtt ccacatcaac
     4861 aggtcctgca aggttcattc gcaacatcgg ccccacggat atcgccatat acgctcgtcc
     4921 ccctggatgg gttggcgtca acgcatgcac caccgcccaa gttgacccca accatccaga
     4981 cgcacataca cctaccgtta tcgactcccc ctcgtgatag caagccccca acccaaacgc
     5041 ggcaagacca aaagaaaatc gttcatcgtc cggttgagat tccgcaacca ccagagactg
     5101 agcgagccag gaccacgacg aaccaagggg catcttcccg accaactcga cggctggtga
     5161 ggcatcggga acgagtccgg ctgccaccgc gccacgcatg ctcaacgacg cttgtccttg
     5221 ggggacaacc tcgcagggct gggatggggg aggcggtttg ggcgtgatgg gttgcgcagg
     5281 cacggccgaa gtgacggtgg tcggcggagg cggtggtggt ggcgcgggag gcggagcctc
     5341 agaggatacc acgatgggtg gttgcaccgg atcggcagag agtttcgcgt ttggatcaat
     5401 gaccaacgcg atggcaagaa cggccgcgga ttgcacggct tcacagtcgg gcgtgtcgct
     5461 ggtcaactcc cgggtgccca cgagattcga cccttctcgc acctggatgc gaacgcgcca
     5521 gccgtgctcc gtgtgctcca cgatcgcttc aatgctacgg cgtgccaaat tggaaaatgg
     5581 cgcggtcgac aagcgtttcg caacgaggtc ttcgatttcc gcgggagaag cgcaaaaatc
     5641 agctccctgt ccacgggccc aggcgaggcg aacaggctcg acggtggccg ctcggctcga
     5701 cgtcatctgc ccgaacaatc cagttccgac gaatcccatg gcgatgacaa ccgttgaaaa
     5761 tgcctttgga aaacccgatg ccatcacggt cagtccttcc gttttgcttc tcgaactccc
     5821 catttccccg cgtcagacaa gccctcgacc gatacctcgc gtagcctcat ttccgctgaa
     5881 tgcgatatca cgatgagaga gactcgcttc atggctcatt gcccaatgcc agtggcggct
     5941 tgccacgctc aagaagaaac acccaacgtc atcaccaacg gcgcatggtc gctcggttgc
     6001 tttcctttgc gcgcttcgcg gtcgatcctc acggactcca cgcgggcgag caacggatgg
     6061 gtcacatacg catgatcgat acgaagcccg tgatttttcg gaaaaccaag agcgcgataa
     6121 tcccaccacg tgaacgcgat ttcatcggga tggagcgctc ggaatgcatc gaatagaccc
     6181 agggaacgaa ggtccgccaa tgcgtttcgt tcccgttccg agcacaagat attaccttcc
     6241 caaaccgtgg gatcgtaggt atctcgaggc tccggagcca cgttgaagtc gccgcaaagc
     6301 gcaatatttg gatacttgcc aatctcttca cgcaacagag cttgaagctt tctcatccat
     6361 tcgagtttgt atacgtactt atccgaatcc agactttgcc cattcgggaa atacgctgaa
     6421 aatacacgaa cttgctcaat tgttgccccg atcaatcgcg cctgcgcatc gccctgcaga
     6481 ccccggtaga tatcctgcat ctcgaaccga ctgatgagcg ccacgccatt gaatgacttt
     6541 tgcccatgca acgccacacg ataccccaga gactcgaagg cgctcgcggg aaattgatcg
     6601 tcaggcgcct tggattcttg catgcaaagc acatcgaccg gatgctgcga gagccaatcg
     6661 accacgcgct ccagacgcgc ccgcacgccg ttgacattcc aggtggcaag ggtcagtggc
     6721 gacgtcatga tcttgtctcg tgaaggggcg gggccatgaa ccctggtccc acgggttcgg
     6781 agatatgtcg gaccacgatt tgatcgatat cggccaattc ttgtgcggat agacgccatc
     6841 cccagacacc atcgagcgga tcgagctgct cgggttttct ggctccccac aaggcgatag
     6901 ggctgccggg ctggtccaac acccagcgta cggcaagttg aatgagagtt tttcctcggt
     6961 cctgcgcaaa acggtcgagt tcatcgacag cggcaagata gtgctggcgt ctcgatggtt
     7021 tgaacttcgg atccacgttg cgtatgtccc cttcaccaaa acgagtgtcg acacggattc
     7081 gaccactgag tagcccacga cataacgcgc catacatcaa ggtggtgatg cggtggtttc
     7141 ggcaaaaagg aagcacatcg cgctcaatct ctcgctcgaa caggttgtac ggcggttgag
     7201 aggtatgcag aggcgcatag tccaaaaaag cggacatctg ttccggcgag taattgctca
     7261 cgccaattgc ccggattttg ccttgctgct gaagacgaag gagcgcctgg gcggtttctt
     7321 gcgccggcac cgctggatcc ggccaatgca cttgttcgat atcgagatgg tcgaccccca
     7381 aacgtctcaa agaatcgtca acctcccgct cgactcgttc tcggctcgca tcccttcgca
     7441 ccttgtcgtc tttccacgcc agtccgagtt tggtggcaat gcgcgcccga gcacggtgcg
     7501 acgaggaggc aagcgctttt cccacgatcg tctcggcttg tccaaagcca tacaccggcg
     7561 ccgtgtcgat cagcgtaatc ccatgatcca atgccttgtg aatcgtacga atggaaagtt
     7621 gttcgtcgct tccaccccaa agccaaccac cgatggccca ggttccaagc gccacacgcg
     7681 atacgtcgat gtcggtgcct tcaatacgta gtatttccat cacgtctgct catttttttt
     7741 ccaaaggacg cagtgtcacc agggttgcgc cccagcttcc gctgtcgata ccgcccagcc
     7801 gataatccac gacttccgtc aggcgatcga ggatggaatg gaccgtcttt cgtagggttc
     7861 ccgtgccttt tccgtggatg attcgaacct cgagaattcc ccgcaggcga cattcttcaa
     7921 ggtaatccgg aaccagattc ttcacatcac gcggctggaa tgtatgcaaa tccaacacac
     7981 catcgatggg aagaacaacg atgtcgtcag aagtcgtcat ggtggcgctg aaccgctgac
     8041 gtaacgcgaa gccacccatg gggcaagtgc ggcccgcgga gtcgttatac cgggggccgc
     8101 cttccacgct tatcagcgaa cgcaacgtcc cttttccgct cggcaccaac cgtttttttc
     8161 acataacaac gacttgcgac aatcaccgtc cgaaccggcc atgcatcggc ctccccgctc
     8221 cgaacaaaga cctttcgaac gacaggaaac cgcttccgca caatcctcgt ccgagttgat
     8281 tcgacaacta ccatgtacgg cggtacacat cccccgctgc ttgcaaatcg ccgtgtcttt
     8341 gcagtcattg tcctgggctt cacacgtgcc atggctttcc gtacacaagc ctcgttttcg
     8401 gcagatttcg gaagcccgac aatccatcgt ggaactcgcc acgcagcgac cataatcacc
     8461 agccgcacac ttaccacttt cacgacataa cggagatcgt cgacaatcat cgtcgaaaac
     8521 cgccacgcac tttccctcct tcgcggagca ttttccctcg tttttgcaga tattcgcgcg
     8581 cgcgcaatcc atgtccgagg ccgccacaca tactccccct tcccccgcaa tgcacaagcc
     8641 ttgctccgca cacgcaagtt tttcccgaca aacacgattg gacgtcgcga cacaaacacc
     8701 atcgcgcgcc tcgcacttgc catgcaaacg gcacgcgtcc gtcagcttgc aatcatagtc
     8761 tttgcgcgct tcacatacgc cgtcgatcga cacacacgcg ccatgctgct tgcatacttg
     8821 agatgactca caatccgaat cctgcgccgc cacacaaacg ccgtgggaca acgtgcattg
     8881 tccccacttt ttgcactcat ccgcgctgcc acacgggtcg gctccacacc ccataaggat
     8941 catcgaaact agccctagca acgacaaaac gcggccccag gccttgatta ccgcggttct
     9001 cactcgtccg caccgctcca taccacgtcg cgatgaaccc agcctttcgc accatcgggg
     9061 ttgacgacat ggagccacga cccctgccga cgcatcacca tcatcccttc tccacggtcg
     9121 gacttgaaca ccacgggact atctttcgta gggttttcac gaacgagggc gcttggcaca
     9181 cgcacgacga cacagctttt cttttccacc aggtgagccg caatccaggc tacatcgcct
     9241 tcaaagtccc tggtttttat ccaatcatcc tttttctcca ccacctcgac cgcgaaatac
     9301 ttgtcggccg tgtacagcac cgcggattgc gtggaaggcc cttcccgaaa attggccttg
     9361 tccaccacaa ccgcaaggat ttccgcccag gccgtccccg acagcaacgt ccctgccacc
     9421 gataaagcaa tccatagacg aggtcgtgtc cgatcgaaaa tggataggaa taacggcttc
     9481 acgacatgat tctccttgtt gaatgatacc cgagggacat tcccggcaac ccatgggctc
     9541 cgcgtccgtc cgcgcctgct gccatgaccg ttacccatgc cctcgctcgc ctcgatgggg
     9601 caaatttcgg acgatgccgc cgcaccccgg tcgagcatct ccctaccctt tgtgcgccgt
     9661 cccgattacc atgcatgaca atgccgccga acgaccatcc cgatgaccgc ctcaccactc
     9721 tggcggggca ttccttttcg tcgcagtacg tccccaatgt ggactaccgc cttggccccc
     9781 aggtagggca aggcgccatg gcagtggcct tctatgccct gcgcaccgct cccgaaggct
     9841 actgcccggt cgtcatcaaa atgctgcgcc cctcgttcgt cagacaattc ggacaagccg
     9901 cctcactcac cgtacaaaaa gaaacggtgg ccttgggacg cttgaacgaa cgaaccccaa
     9961 gcaccccttt cgtcgtacgc ctcatcgata cgggacaatt ggggatttcc gaccgattcg
    10021 gcccccttca attgccgtgg ctcgccgtcg aatacgttca cggaggacca gaaggaacca
    10081 cattgacgca acgcgtcatg aagagcgtac aagatacatc gtttgctttt gacatgaaac
    10141 gagcggcaaa cgccatcgat tgcctatgcg ccgggcttgc ggccgtccac gaagtcggtg
    10201 tcatccaccg cgatatgaaa cccagcaatg tactatgttg tggcaccggc gatgacgaaa
    10261 tcctgaaagt agcggacttt ggggtcgctc gcccccaagg gcttgcaggc actttttttc
    10321 atgatgtcat cggcactctt ggctatgtgg cgccagaggc tttcggcagc cgaggctccg
    10381 aatgcggccc ctggaccgat gtattcggcc tggccggcat cgtttttttc ctgctcacaa
    10441 ggcataagta cctgcgtatc aagaccccag cggacatgct gaccgtcatg ggctctcccg
    10501 aacgccaaag cattctcgaa tctccgatgc tcagcccgga actacggcgc agggagtacg
    10561 cttgtcgcgc catcgacaga gtgctcgccc aatccaccgc cctcgacccc aacgaccgcc
    10621 ccgccagcgt cgaagccttt gccgccatga ttcaaccttg gctgcaaccc gaagcgggaa
    10681 gtcatcgacc ttccccggtt tcccgctccg gcctcgtgcc aacacaagag cgtccagacg
    10741 atgccccccc gtggacgtgg atcc
//
LOCUS       DAOSOR010000141        10696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6165_length_10696_cov_4.080819, whole genome shotgun sequence.
ACCESSION   DAOSOR010000141 DAOSOR010000000
VERSION     DAOSOR010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 10696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10696
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6165_length_10696_cov_4.080819"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PK710_09700"
     CDS             complement(<1..573)
                     /locus_tag="PK710_09700"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOT10034.1"
                     /translation="MDDRPPAMQVEDLIKAMEIIAPTRHAEAWDNVGLLVGDAKASLS
                     RLLLTIDLGIAQVQEAKQLGCEAVIAYHPPIFKEVRRLLSDSPVYEAIRAGIAIYSPH
                     TALDVAQGGTNDSLAEDVLGMRTSEALHLRVDGTGMGKIGVFDPPMPRVQLLERIKKR
                     LALEHVLVAGPQTGVVRNAAVFAGSCGSLVK"
     gene            688..1851
                     /locus_tag="PK710_09705"
     CDS             688..1851
                     /locus_tag="PK710_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10035.1"
                     /translation="MSRTCLGSILAVLLITVACGTSSNETQYSSSAGFRDAGVEASPS
                     SPSDAAKDIQTEDVEQEASPVFDSGFTGDGSINKDVACAVETIQAEEIPVDLFIMADS
                     STSMSGSGWSSLVGGLNAFFTDPVSKGISIALSFFPYDGKCQSADKSCSGDNYFKPQV
                     QWGVLPGHASVLADALAKKSPDGCTPTQDALNGVLRGAFDRQKALQGHTIAAVIVSDG
                     DPCCNMCPIESDSGLGQIAYQYANPAAGEPSIKTFAIYAASEATDSMTAIAINGGTKK
                     AYNATGGTASFIAALDDIRTSLLACEYKMPVPEAGVVNPEVIEVEFTSGTGSKETIPR
                     VKSPQTCGSDPGWRYDDDSAPTRIILCPASCDDVQDDAEGRIDVLVGCTTSQR"
     gene            2003..3226
                     /locus_tag="PK710_09710"
     CDS             2003..3226
                     /locus_tag="PK710_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT10036.1"
                     /translation="MHASADQAGYKAQQGLMWARWNKCEQAIPLLEEAELLRHQAHVS
                     LALADCYASKGKLMEAVELYRALSEEKITPKHLFQDRSAIKKAPQRLADAQARLPTVT
                     FETSEEYPHLEVFLNGVKLDDPFAPKPVAPDMPLSLVVRANGYDELEDEFTLREGEHF
                     VRPIVLTKSVKAEKTSEISVSDEDVSSADPWFGGRGRVFVIPGFFWRIVGDGGRTVFA
                     PGGALSFTTKTGNADLMLTVGYAYYGWKPMPLKGRNAPETDWEIVESSLHNVNATVEL
                     AWRSALDDGGHWEFYWGGGIGLGWALAGDILRTQAYPPDLRPGDPYTYQKCAGPNDPP
                     GSYLYCNELDHDANHYGYAEPNWFAGGKRPFIYPWLAFPQLGITYHPSKSTAVDLETG
                     LTTGGLLLGLGVRAR"
     gene            complement(3335..3967)
                     /locus_tag="PK710_09715"
     CDS             complement(3335..3967)
                     /locus_tag="PK710_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT10037.1"
                     /translation="MTKHRTRDEWVAEILDAAAGRIVAEGYSNLTMESIAAHTSLSKG
                     GVYRYFRNKRDVALALFERVYNNSMDFDYDDIVGWNLSIAETIQRLLVVRRNAEQLRQ
                     DHVIWVQLIPETLWDEGFKQHRTRLFEALRGKFEELVRRIVARDGLVIHPESESRFQH
                     YVYYGLVLREGMTFHSVMGLPLEEQGEMYRQFIELFVKDVFAGQVPTSRS"
     gene            complement(4108..4566)
                     /locus_tag="PK710_09720"
     CDS             complement(4108..4566)
                     /locus_tag="PK710_09720"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOT10038.1"
                     /translation="MTTFKSVEEILDFAIKSEEEAEAFYTGLAAKTESKGMKEAFLSF
                     AKEEAGHKAKLVAIKEGKLDKLLPSFRIVDLKLADYLVDVEATPTMSYQDALIVAMKR
                     EKASYKLYKDLEAKVEDADLKSTFEALANEEAKHKMRFEVEYDEKVLAEN"
     gene            4723..5394
                     /locus_tag="PK710_09725"
     CDS             4723..5394
                     /locus_tag="PK710_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10039.1"
                     /translation="MGTFRGVPIRLHWSIPVGCAVFSGFQFVPAFWLAFTFLVLAHEI
                     GHAIVVRAYRYRVLSIDVTGFGGLCRWSGHANGFERSVIAWGGVLAQLVILLLTLLLI
                     AFFGTPSNPHVSQMAYAFIRSNLMLIGLNLIPVPPLDGAEAWKVFRHGRFQQWWAAMR
                     YRIRKPFPRKPSKGSVIDIGQARKKRQKETQGSTEKTSPNPLADELLRLSKEAAQARK
                     KREEN"
     gene            complement(5411..6985)
                     /locus_tag="PK710_09730"
     CDS             complement(5411..6985)
                     /locus_tag="PK710_09730"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT10040.1"
                     /translation="MMAQPSRVGDLLAGKYRLDALLGSGGVGEVYRAQNTMMGRTVAI
                     KILKPEHVTDGAVVARFMREAKAANLARHPNVVDVLDVGLDDDGVPFIVQEFLEGVDL
                     GKHLKRSKGALGYKQVLELMIPVVEAVGLAHAQGVVHRDIKPENVFLAKVGDTVVPKL
                     LDFGISYIKPQPGDVRMTRTGMTVGTPAYMSPEQLEGTSGVDVRTDVWALGVILYEAL
                     SGHLPFEGETSALLFAQIAWVDPVPLQKIAPQVPEALAQVVSKCLRRKVQERYASATE
                     LARDLRRVRQGKTLDIEPAAKLTGSMPSPCVSTPRARPAQDLIVPVAEPIEPPQVTNK
                     SGLALATLPPPKPDPMPSSPPENVWQDWACGAAVVVEVLCVTGLWMAVVHQPEGWPVH
                     AWLVSSVGGIRSAFGWMFGIAGLGLGAWLIIRHISQPTRYWGWLVMAAGLLLVALPLA
                     GAIFSSAPADAKAPVLWGWGLAIAACGVGLSAVQQAWVTWRSGQAVSKGLGCAWVAAA
                     TLAFLSAFEMIRALSA"
     gene            complement(7064..8182)
                     /locus_tag="PK710_09735"
     CDS             complement(7064..8182)
                     /locus_tag="PK710_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOT10041.1"
                     /translation="MRQYFTLEYGRKLDDFAVGEIYEHPWEVTVDSGMMALFAASFLD
                     ATPVFASRSVARHLGFRDRPASPWLLLNLALSFSVHDVSEQAIAHLSYLDVRFPEPAY
                     AGDTLTASSEVLDVKRSKSAQRGVVHVRTRLQTQDGSVACVFERKALIRAGRLDTRPK
                     TAESLRAPSDIPYPKQPSVLREPTTGSPISTGFFGYFEDFEPGQVFVHAHGRTVGESE
                     HMQLTTLVRNSHPLHFDSVYCEKESFTKRRIVYGGLVLSWVLSLASRDTTGHGLWPVG
                     LDEGAHPNPTHGGDTLFALSRVLSTQAMGPHAGVVQFRVIGVKNEHPVTLLEKGADLF
                     TPEIQKTEGHRVEQKVVEITRAVLVEKRQACNGSHTIA"
     gene            8422..10455
                     /locus_tag="PK710_09740"
     CDS             8422..10455
                     /locus_tag="PK710_09740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT10042.1"
                     /translation="MSILRGVASGILAFAVLWAYGREQIVGTPAAVVFGFLVVGLAAS
                     LVALSLARNRSPLPRFAYINGAIDVTVVTAVLVASFHGSSTTMMLAAALSYLLFSAVS
                     ALRFQLPSSLFTVALAAFEIAGAAIYLHTMMYQPPFVRVMDLILLIIVLVVTGGVVTL
                     SVVGARKSTNASDELERIHRRVLNQVSDGLLVCNSAGRIVDSNAAASDVLGIPRGVLV
                     GLEAYTALPGPLARELEKCWQQALVEGFVLIENLRLGRGQEDTSVLDVAVYRVRYEGD
                     DVIQILLRDATAEQRLLEQTQRSDRLYSLQSLAGGVAHEFNNMFASIEASSYVLAESI
                     PKDAPESMEVEIIRSATARATGLVRELRDLIDTQSPHAVPLDVAALIDRAVTLGKGES
                     NVEVLVHIDSGIKRVLGDEGQLERAFANLVGCSYASMLDGGTLRIHASSDSLSLPKGL
                     APGQYVAITIEDTGIGIPADMVPRAFEPMFSSDGGRWTGLALANARAVITRHGGVISL
                     ESSVGKGTSYFVFLPATDRPAVKALRPVTAPSSHKDKIRLLVVDDEAANRNSLARLLS
                     TRGYEVVLAEDGRRAIEACEQCDGRFDLVLLDLVMPGLNGKDVVVALRARYPSIRVLL
                     VTGFADEQLVEEAMKLGAASFASKPFDVPQLLSQIQQLTSGSRESDRPAMPNP"
BASE COUNT         2306 a         3168 c         2791 g         2431 t
ORIGIN      
        1 cttcacgagt gaaccgcaag atcccgcaaa tacggcagca tttcgcacca caccggtttg
       61 ggggccagcc accaaaacat gctctagggc caggcgcttt ttgatccgct ccaacaactg
      121 cacccgtggc atcggcggat cgaaaacgcc gatttttccc attcccgttc catccactcg
      181 cagatgcagc gcctcgctcg ttcgcatgcc caacacgtcc tccgccagcg aatcgttggt
      241 ccccccctgc gccacatcca atgccgtatg agggctataa atcgcaatcc cggcacggat
      301 cgcttcatat accggcgaat cggagagcag gcgccgtacc tccttgaata tcggtggatg
      361 ataggcaatc accgcctcgc agcccaattg cttcgcttct tgtacttgcg caatccccag
      421 gtcgatcgtc aacaatagcc gcgaaagaga agctttggcg tcgcccacca gcaaaccgac
      481 attgtcccac gcctccgcat gacgcgttgg agcaattatt tccatcgctt ttatcaggtc
      541 ttcgacttgc attgccgggg gtctatcgtc cattcgtgct ttgggaaagc cgccaggctc
      601 gacaaggaca gcctttcgaa atctttgtgg caataccaga aaagaatact attgcctatc
      661 ttctcgcaca tcgcgttgga ggtgattatg tctaggacct gccttggttc cattcttgcc
      721 gttttgctga ttaccgtggc gtgcggaaca tccagcaatg aaacccaata ctcttcttcc
      781 gctggctttc gagatgctgg cgtcgaagca agcccttctt caccaagcga tgctgccaaa
      841 gacatccaaa ccgaagacgt cgaacaagag gcgagccctg tctttgatag tggatttaca
      901 ggggatggtt cgatcaacaa ggacgtggct tgtgccgtcg agaccattca ggccgaagag
      961 attcccgttg acctgttcat catggcggat tcgtcaacca gcatgagtgg cagcggctgg
     1021 agcagtctgg tgggagggct taacgctttt ttcaccgacc ccgtgagcaa gggtatttcg
     1081 atcgccctat cctttttccc ctacgatggg aaatgccaat ccgcagataa aagttgcagc
     1141 ggcgataatt acttcaagcc gcaggtccag tggggagtcc ttcccggtca tgcttctgtc
     1201 cttgcagatg ctttggcaaa gaaatcccct gatgggtgta cacctaccca agacgctctc
     1261 aacggcgtgc tccgaggtgc cttcgatcga caaaaagcat tgcagggcca caccatcgcg
     1321 gcggtcattg tcagcgacgg tgacccgtgc tgcaacatgt gtccgatcga gtcggattct
     1381 ggtctcgggc aaatcgctta ccaatatgcc aatcccgccg ctggggaacc gtccatcaaa
     1441 acgttcgcta tctatgcggc ttcggaggca accgactcca tgactgccat tgctatcaat
     1501 ggcggaacga aaaaagccta caacgcaacg gggggaaccg cttctttcat tgcagcgctc
     1561 gacgatattc ggacatccct gcttgcctgt gaatacaaga tgcctgtacc ggaagcaggt
     1621 gttgtcaacc cggaggtcat cgaggtcgaa ttcacctcag gcactggtag taaggaaacc
     1681 atacctcgcg tcaagtcacc gcagacctgt ggttctgacc cgggatggcg ctacgacgat
     1741 gactctgcgc cgacgcgtat cattttgtgc ccagcctcat gcgatgacgt gcaagatgat
     1801 gcggaaggca gaatcgacgt gctcgtgggg tgtacgacgt cccagcggtg agcgaagacg
     1861 gcttgcgcac ggttgataac aggtctacgc atactctttg ctcaatgggt atcgtgactt
     1921 gatcgcatgc ggctctttcc ggcactggca tgatcgcgga attttcatcg ggtttttctg
     1981 cgcgatggcc gtggtttggc cgatgcatgc ttctgccgat caagcaggat acaaggcaca
     2041 acaggggttg atgtgggcgc gctggaacaa atgcgagcag gccatccctc tgttggaaga
     2101 agcggaactg ctacgccatc aggcacatgt ctccctcgct ctggcagact gctacgcgag
     2161 caagggaaaa ctcatggaag cggtggaact gtatcgtgcg ctttccgagg agaagatcac
     2221 cccaaaacat ctgttccagg accgttcagc catcaaaaaa gccccacaac ggctcgccga
     2281 cgcgcaagct cgactgccca ccgtcacctt cgaaacctct gaggaatacc cacatctgga
     2341 ggtgttcctc aacggcgtga aactcgatga tccctttgcg ccaaagcctg tcgctcccga
     2401 tatgcctctt tcactcgtgg ttcgcgccaa cggatatgac gaacttgaag atgagtttac
     2461 gcttcgcgaa ggagagcatt tcgtacgacc gattgttttg accaaaagcg taaaggccga
     2521 gaaaacaagt gaaataagcg tatccgacga ggacgtctcc tcagcggatc catggtttgg
     2581 cggccgaggc cgtgtgtttg tcatccccgg ttttttttgg cgaatcgttg gtgatggcgg
     2641 acgaaccgtc ttcgcgccgg ggggagcgct gtcgttcaca accaaaacag gcaatgccga
     2701 cctgatgctc accgtaggtt acgcgtatta cgggtggaaa cccatgccct tgaagggacg
     2761 caacgcaccg gagacagact gggaaatcgt cgaaagctct ttgcataacg tcaatgcaac
     2821 cgtcgagctt gcgtggcgtt cggcgctcga tgacggtggg cattgggagt tttactgggg
     2881 gggaggcatc gggcttggtt gggctttggc gggagatatc cttagaaccc aagcgtaccc
     2941 tcccgatctt cgacctgggg acccctacac ctaccaaaaa tgcgcgggcc ccaacgatcc
     3001 tccaggaagc tacctatact gtaatgaact cgatcacgat gcgaatcatt acgggtatgc
     3061 ggaacccaat tggtttgcgg gcggcaaacg cccgttcatc tatccgtggc tagcgtttcc
     3121 tcaactcggc atcacctacc atccttccaa atccaccgcc gtagacctgg agactggttt
     3181 gacaaccggt ggcttgttgt tggggctcgg cgtgcgcgca cgttgatgga gcgtggcatc
     3241 atgacgatca tgtccaacga cccaacgcct tggatgtggc atgagtcatg gcatgggctc
     3301 cgaccccacc atcgctcaaa ccacttgttc aaccttagct tctggatgtc gggacttgcc
     3361 ccgcgaacac gtctttgaca aataactcga tgaactgacg gtacatctcg ccctgctctt
     3421 cgaggggcag ccccatcaca ctgtggaaag tcatgccctc ccgaagcacc agcccatagt
     3481 acacataatg ctggaaccgg gactcggact ctggatggat gaccagacca tctcgtgcga
     3541 cgatgcgtcg aaccaactcc tcgaacttac cgcgcaacgc ttcgaacaag cgggttcggt
     3601 gttgtttgaa cccctcatcc caaagagttt ccggaatgag ttgcacccaa atgacatggt
     3661 cctgacgcaa ttgttcggcg tttcgacgaa ccacgagaag ccgttgaatc gtctcggcga
     3721 tcgataggtt ccaacccacg atgtcgtcgt agtcgaagtc catcgagttg ttgtaaaccc
     3781 gttcgaatag cgccaaagcc acgtcgcgtt tgtttcggaa atagcggtaa acgccgccct
     3841 tgctcaatga cgtgtgggcc gcgatcgatt ccatcgtcag attcgagtag ccttccgcaa
     3901 cgatacgtcc ggccgctgca tccagaatct ccgcgaccca ttcatcgcgt gttctatgct
     3961 tggtcatata atcgctccga cttctcctgg tccgttgtcg aggttaatac tcccacggag
     4021 gccaacggaa gagagccgtt gaagaattag ctgtcgcgtc gcactgaaaa tagcgtttct
     4081 ttcgcggaca atagaaaagg aaggcaatta gttttctgcc aacactttct cgtcgtactc
     4141 aacctcgaaa cgcatcttat gcttggcttc ttcgttagcc agcgcttcga aagtcgattt
     4201 caaatccgca tcttcgactt tcgcctcgag atctttgtac agcttgtacg aagctttttc
     4261 tcgcttcatc gccacaatca acgcgtcttg gtaggacatc gtgggcgtgg cttcgacgtc
     4321 caccaaataa tcggccaact tcaagtccac gattcgaaaa gacggcaaca gcttgtcgag
     4381 tttgccttcc ttgatggcca cgagctttgc tttatgcccc gcttcctcct tggcgaaaga
     4441 aaggaaagcc tctttcatac ctttggattc cgtctttgcc gcaagccctg tgtaaaacgc
     4501 ctcggcctct tcttcactct tgatggcgaa gtcaagaatc tcctccactg acttgaacgt
     4561 cgtcatgcga tgtttctcct catggctgaa aaggtgttct ccaagtagga cgctatcgtc
     4621 gcaaacgcaa gtgaatcctc ggacttcgtt agcacaccgc tttacggagc cttctgccgt
     4681 ggttagcttg ctccggctat gttcgatcga ggcttttggc acgtgggaac gtttcgaggc
     4741 gtccccatcc gccttcattg gtccatcccc gtcggttgcg ccgtcttcag cggatttcag
     4801 ttcgtccccg ctttttggct agccttcacc ttcctcgtac tcgctcacga aatcggtcat
     4861 gccatcgtcg tgagagccta tcgatatcgc gtgcttagca tcgacgtcac gggattcggt
     4921 gggctatgcc gctggtccgg ccacgccaac ggcttcgaac gctccgtcat tgcgtgggga
     4981 ggcgttctcg cccagttggt gattctgctt cttacgctgc tgctgatcgc ctttttcgga
     5041 actccctcca atcctcatgt ttcgcaaatg gcttacgcat tcattcgttc gaacctaatg
     5101 ctcatcgggc tcaacctgat tcccgttcca cccctcgacg gcgcggaagc gtggaaggtg
     5161 tttcgtcatg gacgtttcca acagtggtgg gcggccatgc gataccgaat cagaaaaccc
     5221 ttccctcgaa aaccgtcgaa aggctccgtc attgacatcg ggcaagctag aaaaaaacga
     5281 caaaaagaaa cacaaggatc gacggagaag acctctccca atcctttggc cgacgaactg
     5341 cttcgcttgt ccaaagaggc tgcgcaagcc cgaaaaaagc gagaagaaaa ctaagggatg
     5401 cctgccaacg ctaggcggaa agcgcgcgaa tcatctcgaa tgccgataaa aatgccaagg
     5461 tggcagccgc tacccacgca cagccaagcc ctttgctaac tgcctgcccg cttctccacg
     5521 ttacccacgc ttgttgcacg gcgctcagtc ccacgccgca agccgcaatc gccaatcccc
     5581 agccccacaa taccggtgcc ttcgcgtctg ccggtgccga actaaaaatc gcgccagcca
     5641 ggggcagcgc gaccaacaac aaccctgccg ccatcaccag ccacccccag tatcgagttg
     5701 gctgagaaat atggcgaatg atgagccatg cgccaaggcc gaggcccgcg attccaaaca
     5761 tccaaccaaa cgcggagcga atcccgccaa cgcttgaaac aagccaagca tgcaccggcc
     5821 agccctccgg ctgatgaacg acggccatcc acaaacccgt cacgcacaat acctccacca
     5881 cgacagcggc tccacaagcc caatcttgcc agacattttc cggagggctg gaaggcattg
     5941 gatccggctt gggaggggga agcgtggcga gggccaaccc ggacttattc gttacctgtg
     6001 gcggttctat cggttccgcc accggaacga tcaggtcctg tgcgggcctt gcccgagggg
     6061 tggagacgca aggcgaaggc atcgagcccg tcagcttcgc cgctggttcg atgtcaagcg
     6121 tttttccttg gcgaacccga cgtaaatccc gcgcaagttc cgtggctgag gcgtagcgtt
     6181 cctgtacctt ccttcgcaaa catttgctca ccacttgcgc cagggcttcg ggcacctgcg
     6241 gcgctatttt ttgcaaggga accggatcca cccatgcaat ttgcgcaaat agcaacgccg
     6301 atgtttcccc ttcaaacgga agatggcccg acagcgcctc gtataaaatg actccgagcg
     6361 cccaaacgtc cgttcttacg tccacacccg acgttccctc caattgctcc ggcgacatat
     6421 acgcgggcgt ccccaccgtc attcccgttc gcgtcatgcg cacatcccct ggctggggtt
     6481 tgatgtagga aatcccaaaa tcgagaagct tgggaacaac cgtatccccc acttttgcca
     6541 gaaacacgtt ttctggcttg atatcccggt ggacaacccc ttgcgcatgc gccagaccga
     6601 ccgcctccac cacgggaatc atcaattcga gcacctgctt gtaacccagc gctcctttcg
     6661 aacgtttgag atgctttccc aagtccacgc cctcgagaaa ctcttgcacg atgaaaggaa
     6721 cgccatcgtc atccaaccca acgtccaaca catccacgac attgggatgc ctggccaaat
     6781 tggcggcttt ggcctcacgc atgaatcgcg cgactactgc gccgtcggtg acgtgttcgg
     6841 gttttagaat tttgatggcg acggttcgcc ccatcatggt gttttgcgcg cgatacactt
     6901 cccctacgcc accacttccc agcagcgcat cgagtcgata tttcccggcc agtaggtcgc
     6961 ccacacggga gggttgggcc atcatcgcaa tcatacgtca gtacgtttgg gtacgcatag
     7021 aattggggaa tcttacggca aaacacgccg catagcacga agatcaggcg atggtgtgcg
     7081 aaccgttaca agcttgccgt ttttcaacca ataccgctcg cgtaatttct accaccttct
     7141 gctccaccct atggccttcc gttttctgga tctcgggtgt aaatagatcg gccccctttt
     7201 cgagcagcgt gactggatgc tcgtttttta ccccgatcac tcgaaattgg acaactcccg
     7261 catggggccc catcgcttgc gtgctgagga cgcggctcag tgcgaacaac gtatcgcccc
     7321 cgtgcgtggg attgggatgg gccccttcat caagcccaac aggccacaac ccgtgacccg
     7381 tcgtatctcg actggctagg gacaacaccc agctcagcac cagaccccca tacacgatgc
     7441 gacgtttcgt aaacgattct ttttcacaat acacggaatc gaaatgcagc ggatgggaat
     7501 tacgaacgag cgttgtcagt tgcatatgtt ctgattcacc gacggtgcga ccgtgggcat
     7561 gaacgaaaac ctgccccggc tcgaaatctt caaaatagcc gaaaaaacca gtacttatgg
     7621 gcgatccggt cgtcggttcg cgcaacaccg aaggctgctt gggataagga atatcgctcg
     7681 gtgcccgcag actttccgcg gttttcggac gcgtatcgag ccgacccgct cgtatcaacg
     7741 ctttgcgttc aaaaacgcat gctactgaac catcttgtgt ctgcaaccgc gttcgaacat
     7801 gaactacccc tctttgcgcc gacttcgacc gcttcacgtc cagcacttcc gacgatgccg
     7861 tcagggtatc cccagcgtaa gcgggctccg gaaaccgaac gtcgagatac gataaatgcg
     7921 caatcgcttg ttcactgacg tcatgcacgg aaaatgacag ggcgagattc agcagcagcc
     7981 atgggctcgc ggggcgatca cgaaacccga gatggcgagc taccgaccgg cttgcaaaca
     8041 cgggtgtggc atcgagaaac gatgcggcaa atagcgccat cattccgcta tccaccgtga
     8101 cttcccatgg atgctcgtag atttcgccga ctgcgaagtc atccagcttt cgaccgtatt
     8161 ctaacgtgaa gtattgcctc atccctcgtt tccttctgtc ttgtgtcgac gtgcgacagc
     8221 gtggcattgc gctaattcgg catctttttc gactcgaaat cctgtcctgg cgagcaggga
     8281 acggtcgatg cgaaaccagg aaattgtcca cgcgtgacga atgaaagcaa cctgcttaca
     8341 atggcctgaa cgctcatgga cttttcccag catgaacctg gatggaaatc gtttttggac
     8401 gagtcgaaag ctgaggaacg cgtgtccatt ttgcgcggtg tggctagcgg aatcctggcc
     8461 ttcgcggtgc tctgggccta tgggcgggaa cagatcgtcg gaactcccgc tgcggtcgtg
     8521 tttggttttc tcgtggttgg ccttgcagca tcgctcgtgg ctctatcgtt ggcccgaaat
     8581 agaagcccac tgcctcgctt cgcctatatc aacggagcca tcgatgtcac cgtcgtaacc
     8641 gccgtgctcg ttgccagttt ccatggatcg agcactacga tgatgcttgc ggccgctctt
     8701 tcgtacctgc ttttctccgc agtttccgcc ttgcgtttcc aactcccctc atcgctgttt
     8761 acggtggcgt tggccgcatt cgaaatcgcg ggggccgcca tctatctgca taccatgatg
     8821 tatcaaccgc cgtttgtacg cgtgatggat ctgattttgc tcatcatcgt tcttgtcgtt
     8881 acaggtgggg tcgtgaccct ctctgtcgtt ggcgcgcgga aaagcacgaa cgcatccgat
     8941 gaattggagc gaattcatcg acgagttctc aatcaagtat ccgacggcct gctggtttgt
     9001 aattctgctg ggcgcatcgt cgattccaac gccgcggcca gtgatgtgct cggaattcct
     9061 cgtggcgtac tcgtaggcct cgaggcgtat acggcgttgc ctggtcctct tgctcgtgaa
     9121 ttggaaaaat gctggcagca agcgttggtg gaaggatttg tcctcatcga aaatcttcgg
     9181 cttggccgag gacaggaaga tactagcgtt ctcgatgtcg ccgtgtaccg cgtgcgatac
     9241 gaaggtgatg atgtcataca gatcctgctc agggatgcca ccgccgaaca gcgtctactc
     9301 gaacaaaccc agcggagtga ccgtttgtat tctcttcaat cgctagccgg cggcgttgca
     9361 cacgaattca acaatatgtt tgcaagtatc gaggcctcct cgtacgtgct cgccgaatcc
     9421 attccgaaag atgcaccaga atcgatggaa gtggaaatca tccgcagcgc aaccgctcgg
     9481 gccaccggat tggtccgcga attgcgagac ctcatcgata cgcaatcccc ccacgccgta
     9541 cctctcgatg tggccgcgct catcgaccga gcggtgacgc ttggcaaggg ggaaagcaat
     9601 gtcgaggtcc ttgttcacat cgattccggg atcaaaagag tcctcggcga tgaaggacaa
     9661 ctcgaacgcg cttttgccaa tctcgtgggg tgttcctacg cttccatgct cgatgggggg
     9721 accttgcgga ttcatgcctc gagcgattcc ctttcccttc ccaaggggct ggctccagga
     9781 caatacgtag cgatcaccat cgaagatacg ggaatcggta ttccggccga catggtgccg
     9841 cgcgccttcg aaccgatgtt ttcttccgat ggtggtcgtt ggacgggctt ggccctggcg
     9901 aacgcacggg ccgtcatcac acgtcatggg ggcgtcattt ctctcgagtc cagcgtagga
     9961 aaaggcacct cctatttcgt ctttctcccc gctaccgacc gtcccgctgt caaagccctt
    10021 cgcccggtga cggctccctc ttctcacaag gataagattc ggctgctggt cgtcgatgat
    10081 gaagccgcca atcgaaactc cctggctcgt ctactatcca cgcgcggtta cgaagtcgtg
    10141 cttgcggagg atggacgccg tgccatcgaa gcgtgcgaac aatgtgatgg gcgattcgat
    10201 ctggtgctgc tcgacctcgt gatgccgggg ctcaatggca aagacgtcgt tgtcgccttg
    10261 cgagcacggt atccaagcat ccgtgtgttg ctcgtgacag ggttcgccga tgaacaactc
    10321 gtggaggagg ccatgaagtt gggcgcggct tccttcgcct cgaagccctt tgatgttcca
    10381 caattacttt cgcaaattca acaacttaca tccggttcaa gggagtccga ccggccggca
    10441 atgcccaatc cataacgctt tgttcctcgc tggatctgac atcaacggcg tatgacgcgc
    10501 ccgcgaaaac gcaaactgcg gcgggtcgtt cggcaaccat cttccctgct cgaagcgtca
    10561 tgctcgaagt cgttacggct ccttattttg cctcggcaga atatatcgct tttgccatgg
    10621 cttgggactg gccggaggcc gcgccgtctg cttcgccacc ggattcggtg atatgctgga
    10681 aagtcacgcc atgtgt
//
LOCUS       DAOSOR010000142        10661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6205_length_10661_cov_4.084292, whole genome shotgun sequence.
ACCESSION   DAOSOR010000142 DAOSOR010000000
VERSION     DAOSOR010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 10661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10661
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6205_length_10661_cov_4.084292"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="PK710_09745"
     CDS             complement(<1..1018)
                     /locus_tag="PK710_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10043.1"
                     /translation="MSWFTKFEEGSRTILLVAQSSASMRRRFALGAVGLLVLAGAGGY
                     FLHKHSTQQAQAQVGSSWASLDQCLLGAPLAEGEKPSMRFRAVQLSALSQSVVEAGGE
                     KAQWPVRCATHAHALRDGLVAISGTPVEKSLAYWVDKLAAALSATGASSADLSEIVDA
                     TWEQAGREGMARDKGQVTGPEAPLPTKALTIDDLKSHKPIVSKAPALDTLQTDVHPSA
                     VLNLFAEDANKEHSLFCSMSPEKKVIHCEPLAAGIPASQLGMRLLGTSDDDVAPLLFA
                     GSRGSEGVYRIVGGDKITASTALGGYVTKDVASVLTWDEGSKRILLHRKTGADEPKSV
                     PVRLD"
     gene            complement(1100..2626)
                     /locus_tag="PK710_09750"
     CDS             complement(1100..2626)
                     /locus_tag="PK710_09750"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT10044.1"
                     /translation="MDAPAKAGDIIDGRYRLERVVGSGGMAVVVAAIDIERSERVALK
                     FLFKKAARHKEILQRFEREKSVIGRLEGEHVARMLGAGIVKNTPYMVMEYLQGRDLAD
                     LLRVQQTLPVEQAVEYILQACEAVAEAHGLDIVHRDLKPGNLFLTHRPDGTPCIKVLD
                     FGIAKILGGGDQEEKSLTRTEVVMGSPNYMSPEQMISAKDATPATDIWALGVILHELV
                     AGTLPFAERSTERLCARVLQGEPTPLRKDCPSCPEQLENVVLRCLQRMPQDRFGDIAE
                     FAKALAPLAPESAQALVPRIEKRLRQPRVSLTEEQLSSLVPARNPEEASEAPTVYLPK
                     PAQLPWKFIVFVGFGLLMAGLGFAGGLVFQRPVQPAAPDSIAATTASSHSVPASSSAP
                     LTVVEGAEHDDHELVQPIEIDLDDMVKKESDTVRRKPAASASSSPRSSPTSSSVPRST
                     PSASSPETVPTRDAAPAPTVSTAPASSASVAPPNVSTKPSSTAPPAPSPPKTESDSPY
                     "
     gene            2959..3657
                     /locus_tag="PK710_09755"
     CDS             2959..3657
                     /locus_tag="PK710_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOT10045.1"
                     /translation="MSGRHDPNSFKAYLDEICERLDRNEPLRRTRKAWWIVPVAVGLT
                     LACGGKATTEDDPAGGAGEAGSGGSGGANTGGSNTGGSNTGGSNTGGSNTGGSNTGGA
                     NTGGSGAATGGTGALYAAPLENCYDNEDNDFDGKVDCDDEDCFDAPICSGIEYAAPIE
                     NCYDGEDNDFDGKVDCTDEDCFDAPVCSGTDYAAPFEDCSDDKDNDFDGKVDCEDDDC
                     DNDPMCMGELYAAP"
     gene            3784..5187
                     /locus_tag="PK710_09760"
     CDS             3784..5187
                     /locus_tag="PK710_09760"
                     /inference="COORDINATES: protein motif:HMM:NF028290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOT10046.1"
                     /translation="MTVPWWGILPFVLLLACIATFPLIPLTKKLWHHNWFQLLVALAC
                     GVPMGIWMWVGGDSEKVIHALVEYASFITLLLSLFVVSGGISVGGDIRATPRNNVIFL
                     LIGGTLASFIGTTGAAMLMIRPLLNINSQRKNKVHTVVFAIFVIANCGGLLTPLGDPP
                     LFLGMLRGVPFLWTMHLLPEWLFTNGMLLMTFFAIDRKFYSQEPALAISLDDREIQPI
                     RIVGKLNFLWLACIVLAVALAPSVDLHAIFEGHAHWNHWIPFREIVMLSVAGISLASS
                     DKKVRYEDNKFEWGPIMEVAALFIGIFLAMIPALTYLAQVAPTLPLNEVTFFAFTGTL
                     SSVLDNAPTYVTFFEMAKTLPGQPRVADVPEIYLVPISLGAVFCGAITYIGNGPNFMV
                     KSIAESADVEMPSFGGYTLKWAFRYLLPILLIMVMIFVVQDWRIRGLGIFFAVLRVAQ
                     EIWQASRYPDPHKHKAT"
     gene            complement(5444..6712)
                     /locus_tag="PK710_09765"
     CDS             complement(5444..6712)
                     /locus_tag="PK710_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOT10047.1"
                     /translation="MASEGKNYKVADIGLADQGRMKIEWAESRMPVLMALREEHAKKQ
                     SFKGARIAGCLHVTKETAVLVRTLRAAGAEVSWSGCNPLSTNDEVAAFLAADGASVYA
                     WHGQNVEEFYWCIDRTLDFKPTLTLDDGADLIFTVHNRHKELAKTIIGGTEETTTGVH
                     RLRAMAADGKLMYPVFAVNDSETKWDFDNVYGTGQSSLDGILRATSVLVAGKTFVVAG
                     YGHCGRGCAMRAKGLGANVVVTEVKPTAALRATLEGFRVMPMDEAAKIGDIFITATGM
                     KDVLVKHHFESMKDGAIVCNTGHYDCEINLVQLGGIAQAKRTIRPNNEEYMVQGKRIY
                     VLAQGRLVNLAAAEGHPSEVMDMSFANQFNALLALHQKGPGGYPLGVHVLPAELDQQI
                     AMLKLKTQGVGIDTLTAEQIAYATDYSAGT"
     gene            complement(6702..7691)
                     /locus_tag="PK710_09770"
     CDS             complement(6702..7691)
                     /locus_tag="PK710_09770"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT10048.1"
                     /translation="MTSPLAIRFRALADPTRLLLLGVLLDEELTVGELAEVSRTRQPS
                     VSRHLAGLRDAGLIVSRRQGAMTFYRANAQEVLVTGALRAEILSLVQNPTIAGRIESV
                     LERRRARADEFFDERAEGWDGLRAQLLSDSAVFASFLPLIPRGLTVVDIGTGTGGLLP
                     HLAEFSDRIVGIDQSAQMLRRARDRARRLGLTNVEFHRADISKLPLPSNTFDVAFAVL
                     VLHHAPKPLVAMREMARVVKPGGRVIVLDLVAHGQEWLRREQADLWLGFTAEEVKAMM
                     DGASLHLGGRRVISRAKSPRSEESLELFVAWATKQQEQTALSKRGRGDIDHGQ"
     gene            complement(7852..9012)
                     /locus_tag="PK710_09775"
     CDS             complement(7852..9012)
                     /locus_tag="PK710_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10049.1"
                     /translation="MNSLWQRLIPFVIGLAVVLVLRWLSKNKFRPATYEMSLEEEGYL
                     VEYQPGSKKTPSRLTFSIKDSQRRDPTAGKGMVPYRSGPKGPEILTYVSPLVLRKETK
                     WDRLGKALGLNREVQTREASFDRAVYIETDAPAPLVRKILDDEKVRSQVHQWLDDGWE
                     KVVFFGMGTSVGLEKRGVAKPQALDPRCQQWMRELSSVSRRLPQVRYEHIRMAPWTRR
                     DWAKTISAGLAVLGVTATVVAREAYPPLFWRPFEVCIGLGVVGWLAAVPLLALAFRGR
                     SDSLRVFGTCSLLLALGLPTTMYGLGLGLNGLLDGSPAVVKQLQVEKAWATRSKSTSY
                     HVTLKHWNEWVEPFSLQIDGSTYYGIQRHLPPQYVALTIRGGGFGVAVVDAS"
     gene            9444..>10661
                     /locus_tag="PK710_09780"
     CDS             9444..>10661
                     /locus_tag="PK710_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT10050.1"
                     /translation="MRFSSRFVPVLVVVLGSIVGCAGSDDATAEPSEMDSSTDSHEQD
                     ENTTEEGGQENPDSGTSLTDSGSLEADSADCVDECEIDGERACHEDGIRTCGHYDTDS
                     CLEWSPVEACPEQQTCDPSTVTCQEDEGEPLAPFSIILIPDPQNYTGLKNDANNPYRK
                     QTQWIADHWVSDRIQTVIHLGDNTNNNKKSEWAVGDAAFDTLDKAGRRYSMTTGNHDY
                     RVDGVFKRNGSLFDNYFGKSRFEGKPWYGDSYGNSNINNYTKFEVGTMKFLVLSLEFA
                     PRKEVLEWGSKVIGNHPDHHVIIATHCYLTHDGKYSSCKASDKVVGSSGDTTFKELAA
                     RHSNVFLVVSGHINDSEYRMAKGNNGNPVHQMVVDYQMEKPCAEAKAANCKNHCRSGG
                     HTGNGWLRKLIFDP"
BASE COUNT         2362 a         3263 c         2775 g         2261 t
ORIGIN      
        1 catcgagcct gaccggcacg gacttcggct cgtccgcccc tgtcttgcga tgcaacaata
       61 tccgtttgct cccctcatcc cacgttagta ccgaagccac atctttggtg acataccccc
      121 ccaatgccgt cgatgccgtg attttatccc ctcccacgat ccggtacacg ccttcgctgc
      181 ctctcgaccc ggcaaataac aagggagcaa catcatcgtc actcgttccc aacagccgca
      241 ttcccaattg cgaagccgga atgcccgcgg ccagcggttc gcaatgaatc accttttttt
      301 ccgggctcat ggagcagaat aacgagtgct ccttgttggc atcttcagcg aaaaggttga
      361 gcacggcgga tggatgcaca tctgtctgca gggtgtcgag ggcaggagct ttgctcacga
      421 tcggcttgtg cgacttgaga tcgtcgatgg tcaacgcctt cgtaggcaag ggagcttccg
      481 gtcccgtgac ttgccctttg tctcgcgcca tcccttcgcg gcccgcttgt tcccacgttg
      541 catccacaat ctcactgagg tccgctgaac ttgcgccagt ggccgaaagc gccgcggcga
      601 gcttgtccac ccaatacgca agactttttt ccacgggcgt tcccgatatc gccaccaatc
      661 cgtcgcgaag cgcatgcgcg tgagtggcac accgtacagg ccactgcgcc ttctcacccc
      721 ctgcttccac caccgattgc gacaaggccg aaagctgcac cgcccgaaat ctcatgctcg
      781 gcttttctcc ctcggcaagc ggagcaccga gcaaacactg gtccagcgac gcccatgatg
      841 aacccacctg cgcctgggct tgttgtgtgg aatgcttgtg aagaaagtac cctcccgcac
      901 ccgccaacac caaaaggcct acggctccaa gcgcaaaacg tcgacgcatc gacgccgacg
      961 attgagccac cagcaatatc gttcgcgagc cttcttcgaa ttttgtgaac caactcatga
     1021 gcggatcatc taggaaagcc aaggagacgg caagcttcgt gcaaaccaaa cctcttctaa
     1081 cattcctcga tttctgtttt cagtacggcg aatccgattc cgtttttggg ggcgatggag
     1141 caggaggcgc ggtcgacgat ggtttcgtgc tcacgttggg cggcgcgacg gaagcgctgg
     1201 aggcaggtgc cgtggacacg gtcggcgctg gagccgcatc acgggttggg acggtttccg
     1261 gtgacgatgc ggagggggtg cttcgcggca ccgaggaaga agtaggggaa gaacgtggcg
     1321 aggaggaagc ggatgcggct ggctttcgtc gcaccgtatc cgattctttt ttcaccatgt
     1381 catcgaggtc gatttcgatg ggctgaacca actcatggtc atcgtgctcc gctccctcca
     1441 cgaccgtgag gggtgcggag ctagaagcgg gcacagaatg gctcgaagcc gtcgtggcgg
     1501 cgatggaatc cggagcggca ggctgcacgg gccgttgaaa tacaagcccc ccagcgaatc
     1561 caaggccggc catgagcaga ccaaacccca cgaaaacaat gaatttccac ggtaactgcg
     1621 cgggtttggg caaatacacc gtcggcgctt ccgaagcttc ttccgggttg cgtgcaggca
     1681 ccaagctcga gagctgctct tcggtcagcg aaacacgcgg ttgcctgagc cttttttcaa
     1741 tccggggcac cagcgcctgc gcggattccg gagccaaggg agcaagtgct ttggcaaatt
     1801 ctgcaatgtc cccaaatcgg tcttgcggca tccgctgcaa acaacgaaga accacgtttt
     1861 cgagctgttc cggacaactt ggacaatcct tccgaagggg cgtcggctcc ccttggagca
     1921 cacgcgcaca aagccgttcc gtcgaacgct ccgcaaacgg cagcgtcccg gccaccaact
     1981 catgcaaaat gactccaagg gcccatatat ccgtcgcggg cgtggcatcc ttggccgaaa
     2041 tcatttgctc cggcgacata tagttgggag aacccatcac gacttcggtc cgcgtcaacg
     2101 atttttcttc ttggtcgccg cctccgagga tcttggcaat tccaaaatcc agcaccttga
     2161 tgcatggcgt tccgtccggc cgatgcgtca aaaacagatt gcctggcttg aggtctcggt
     2221 gaacgatatc tagcccatgc gcttcggcca ccgcttcgca agcctgaagt atgtattcaa
     2281 cggcttgttc gacaggcaac gtctgctgaa ccctcaacaa atctgcaaga tcccttccct
     2341 gcaagtattc catcaccata taaggggtat ttttcacgat tcccgccccg agcatccgtg
     2401 ccacgtgttc cccttcgaga cgaccgatca cggatttttc ccgttcgaag cgttgcaaaa
     2461 tctccttatg tctcgcggct ttcttgaaaa ggaacttgag agcgacccgc tcggagcgct
     2521 cgatatcgat ggcggcgacg accacggcca tgccgccgga gccgaccacg cgttcgagcc
     2581 ggtaccgacc atcaatgatg tcacctgctt tagccggtgc gtccatggat ccttgctccg
     2641 tgcctcatgc gcggctgccc atgactggca cggctccata cagcctggat tctaagggat
     2701 ccgcattacg atggccagaa aggccatgcc ctcgccgacg aacatcggcg cggctcccaa
     2761 tcgattcact tctttccccg attatccctg gtcgattgac ctcttttccc ttcaccccga
     2821 gagcaaaaag aggggcccgg cgatcccttg cacttccgag gctcaatccc ttgctttgcc
     2881 ttttccacgg aacgcattgt catgctactc tcatggtatc tttccataac gcgatgcttg
     2941 cggaaaagga gcacgacgat gagtgggcgc catgatccaa acagcttcaa agcttatctc
     3001 gatgagattt gcgaacgcct cgatcgaaac gagccgctgc gacgcacgcg aaaggcttgg
     3061 tggatcgttc ctgttgccgt cggcttgacg ctcgcttgtg gaggaaaggc gacgacagaa
     3121 gacgaccccg cgggaggagc aggcgaggcg ggctcgggcg gaagcggcgg agccaatacc
     3181 ggcggtagca acactggcgg tagcaacact ggcggtagca acacgggcgg cagcaacacg
     3241 ggcggcagca acaccggcgg ggcgaatacc ggtggaagcg gcgcggccac gggaggcact
     3301 ggtgccctgt acgccgctcc cctcgagaat tgctacgaca acgaagacaa cgatttcgat
     3361 ggcaaggtgg attgcgacga cgaagactgt ttcgacgcgc ctatttgctc cggtatcgaa
     3421 tacgctgcac ccatagaaaa ctgctacgac ggggaggaca acgacttcga cggcaaggtg
     3481 gactgcaccg acgaagactg tttcgatgcg cctgtctgct caggaacgga ttacgctgct
     3541 cccttcgaag actgttcgga tgacaaagac aacgacttcg atggcaaggt ggactgcgaa
     3601 gacgacgact gcgacaacga tcccatgtgc atgggagaac tctacgctgc cccctgattc
     3661 ccaccgccgg acatctttgt gaaacccatg gcggcattct ttgtgtggtt gccgcacttt
     3721 cgctatgctc gcggccatcc ataaggtgac cctataaccc ccgtcaccgg tatgaggaca
     3781 acgatgaccg tgccctggtg gggaatcctc cccttcgtgt tgctccttgc ctgtatcgca
     3841 acctttcccc tcatcccgct caccaaaaag ctttggcacc acaattggtt ccaattgctc
     3901 gtggctctcg cttgcggcgt gcccatgggc atctggatgt gggtcggcgg agatagcgaa
     3961 aaagtcattc acgcgctggt cgaatacgct tcgttcatca ccctgctgct gtccctattc
     4021 gtcgtgagcg gaggcatctc cgtagggggc gacatccgcg caacccctcg taataacgta
     4081 atatttttac tcatcggcgg aacgctcgct tcgttcatcg gcaccacggg cgcggccatg
     4141 ttgatgattc gtcccctgtt gaacatcaac agccagcgaa aaaacaaagt ccataccgtc
     4201 gtattcgcca tcttcgtcat cgccaactgc ggtggattgc tcacgcccct gggcgaccct
     4261 cccctgtttt tgggaatgct gcgtggcgtg ccgtttttgt ggacgatgca tctattgccc
     4321 gaatggctat tcaccaatgg catgctgctg atgaccttct tcgccatcga ccgcaaattc
     4381 tattcgcagg aacctgcctt ggcgatcagc ctcgatgaca gagagattca acccatccga
     4441 atcgtgggca aactcaactt cctttggctg gcttgtatcg ttctcgcggt tgccctggct
     4501 ccctcggtgg acttgcacgc catcttcgag gggcatgcgc attggaatca ttggattccc
     4561 ttccgagaga tcgtcatgct ttcggtggcc ggcatctccc ttgcgtcgtc tgacaaaaaa
     4621 gttcgctacg aggacaataa gttcgaatgg ggtccgatca tggaggtggc cgcgttattc
     4681 atcggcattt tcctcgccat gattcctgct ctgacgtacc tggctcaggt tgcccctacc
     4741 ctgcctttga acgaagtgac attttttgcg ttcaccggca cgttgtcctc cgtactggat
     4801 aacgcgccca cctacgtcac ctttttcgaa atggcgaaaa ctctgcccgg acagccgcgc
     4861 gtcgccgatg tccccgaaat atacttggtt cccatcagcc tgggcgccgt gttttgtggc
     4921 gcgatcacgt atatcggcaa cggccccaac ttcatggtca agtccatcgc cgaatccgcc
     4981 gacgtcgaga tgccttcctt cggcggctac accctcaaat gggccttccg ctacctgctg
     5041 cccatcctgc tcatcatggt catgatcttt gtcgtgcaag attggcgcat tcgtggcctg
     5101 ggaattttct ttgccgtcct tcgcgtcgcc caggaaatct ggcaagccag tcgctatccc
     5161 gatccgcata aacataaagc tacataagat ggctactaca catttttctg tgtaattaca
     5221 gcatactaca ccaataccat ctcttgaaac catcagcttt ccacgttatc cgaatacgta
     5281 tcatcggaca tggaaactcg aaggagttcg ggagaaacgg cggcgtccgt gccaacaatt
     5341 tcgccgttgg cgattcggaa aacgctacga cgagttgctg attcgagggg acaggtccag
     5401 gaaatcggca atcagggtgt cgaaagagga tgagccgcag cgctcatgtt cccgcggagt
     5461 aatcggtggc gtacgcaatc tgttcggctg tcaaggtatc gatacccacg ccttgggtct
     5521 tgagcttgag catggcgatt tgctgatcga gttcagccgg aagtacgtga accccgagcg
     5581 gatagcctcc cggtcccttt tggtgcagcg cgagcagagc gttgaattgg ttggcaaagc
     5641 tcatatccat cacttcgctg ggatgacctt ccgcggccgc cagattgacc aaacgcccct
     5701 gcgcgagcac gtaaatgcgc ttgccctgaa ccatgtactc ctcgttgttg gggcgaatgg
     5761 tgcgctttgc ttgcgcaatc ccaccgagct gcaccaaatt gatctcgcag tcgtaatgcc
     5821 ccgtgttgca cacgatcgca ccgtctttca tgctctcgaa gtgatgcttc acgagcacat
     5881 ccttcatgcc cgtcgcggtg atgaagatgt ccccaatttt ggcggcttca tccatcggca
     5941 tcacacggaa cccttcgagc gttgcacgaa gggcagcagt cggcttgacc tccgtgacca
     6001 ccacgttggc cccaagcccc ttggcacgca tcgcgcatcc acgaccacaa tgcccatagc
     6061 cagccaccac gaatgtcttg cccgccacaa gcacactggt cgcccgaaga atcccgtcga
     6121 gagaagattg cccggtgccg tatacgttat cgaaatccca cttggtttcg gaatcgttga
     6181 ccgcaaacac cggatacatc aacttgccgt ccgcggccat ggcgcgcaga cgatgcactc
     6241 ccgtggtggt ttcctccgtg ccgccaatga tggttttcgc aagctcctta tgacgattat
     6301 ggacggtaaa aatcaggtcc gcgccgtcgt cgagcgtcaa cgtgggtttg aaatcgagcg
     6361 tgcggtcgat gcaccaataa aactcttcga cgttctggcc gtgccacgca tacaccgacg
     6421 cgccatcggc ggcgaggaat gccgccacct catcgttggt ggacagcgga ttgcatccgg
     6481 accaggaaac ttcggcgcca gcagctcgca aggttcgcac gagaacggcg gtttccttgg
     6541 tgacatgcaa gcaccccgca atgcgagcgc ctttgaagga ctgctttttc gcatgctctt
     6601 cccgcagggc catgagcacc ggcatgcgtg actccgccca ttcgattttc atgcggcctt
     6661 gatccgcaag cccaatatcc gcaactttgt aatttttacc ttcactggcc atgatcaatg
     6721 tctccccgac ctcgcttgga caaggccgtt tgctcctgct gtttcgtggc ccacgccacg
     6781 aataactcca atgattcttc acttcgaggg ctctttgcgc gcgagatcac ccggcgcccc
     6841 cctaggtgca acgatgcgcc gtccatcatc gctttgactt cctcagccgt aaaaccaagc
     6901 cataagtctg cttgctcccg acgcaaccac tcctgcccat gggctaccaa gtccaggacg
     6961 atgacccgcc cccccggttt caccacgcga gccatctccc gcatcgcaac caaaggcttg
     7021 ggcgcgtgat gcaacaccaa gaccgcaaac gccacgtcga aggtattcga aggcagcggc
     7081 agcttcgaga tgtccgctcg gtggaattcc acgttcgtca ggcccaggcg acgtgcccta
     7141 tcacgcgcgc gccgaagcat ctgagccgat tgatcgatgc ccacgatgcg atccgaaaac
     7201 tcggccagat ggggcaacaa tccacccgtg cccgtaccga tgtccaccac cgtcaagccc
     7261 cgcgggatca acggcaaaaa cgatgcaaat accgcggaat ccgacaaaag ttgcgcacgc
     7321 aaaccgtccc acccctccgc tcgttcatcg aaaaactcgt ccgcccgtgc tcgacgccgc
     7381 tcgagtacgc tttcgatgcg gcccgcaatc gttggatttt gtaccaacga aaggatctcg
     7441 gcacgcaaag cccccgtcac caatacttcc tgtgcgttgg ctcgataaaa cgtcatcgcc
     7501 ccttgtcgcc gggacacaat caatcccgcg tctcgcaacc ccgccaaatg ccgggaaacg
     7561 ctcggctggc gcgtccggct cacttccgcc agctcgccca ccgtcaattc ctcatccaac
     7621 aaaaccccta gcaaaagcaa gcgcgtggga tccgcaagcg cgcgaaatcg aatggccaag
     7681 ggggacgtca tgtcgtcatt cgcatattcg aacatatacc aacgcgcaag gatattgttt
     7741 cctcattcgt cccgaagtgc atggatgagc cgttcaagcc cccccgagac gatgtcgcgc
     7801 tcatccccag cctatcggcc gatatcgacg tgcttcggtt cttgcaccgg atcaagatgc
     7861 gtccaccacg gccaccccaa agcccccccc ccgaatcgtc agagccacgt attgaggcgg
     7921 gagatgtctc tggataccgt aatacgtgct gccatctatc tgaagggaaa acggttcaac
     7981 ccactcattc caatgcttca acgtcacgtg ataagacgtc gacttgcttc tcgtcgccca
     8041 ggctttttca acttgcaatt gcttgacgac cgctggcgag ccatcgagca gtccattgag
     8101 cccaaggccc aacccataca tcgtggtcgg cagtcccagc gccagaagca gcgaacaagt
     8161 tccaaacaca cgcaaagaat ccgagcgacc ccgaaaggca agtgccaaca agggaaccgc
     8221 cgccagccac cccacgaccc caagaccaat gcatacctcg aaagggcgcc aaaataacgg
     8281 cggatacgct tcccgggcga caaccgtggc ggttactccc agcaccgcca acccagccga
     8341 aatcgtcttc gcccaatccc gtcgcgtcca gggtgccatc cgaatatgct cgtaacgaac
     8401 ctggggaagc cgacgagaga cggacgatag ctcccgcatc cattgttggc agcgcggatc
     8461 gagcgcttgg ggttttgcca ctccccgttt ttcgagcccc accgacgttc ccatcccgaa
     8521 aaacaccacc ttttcccaac cgtcatcaag ccattgatgc acctgagatc gcaccttctc
     8581 atcatccaag atcttgcgaa ccaaaggagc tggagcgtcg gtttcgatgt ataccgcacg
     8641 gtcgaaagag gcttcccgcg tttggacctc ccggttgaga cctaacgcct ttcccaaccg
     8701 atcccatttc gtctccttgc gcagcacgag gggagagaca tatgtcaata tttccggccc
     8761 cttgggcccg gagcgatagg gaaccatccc ctttccggct gtcggatccc ttcgctgcga
     8821 gtctttgatc gaaaaggtga gccgcgacgg cgtctttttc gaacctggct ggtactccac
     8881 caagtagcct tcttcctcga gcgacatttc gtaagtggcc ggacgaaact tattcttcga
     8941 taaccaacga agaaccagca ctaccgcaag cccgatgacg aaaggaatca acctctgcca
     9001 taaggaattc atcgaatacc cttgagattg gttttcacaa acacctgccc cgaaatcgta
     9061 tcaccggaat cactaaacca gttgaatggg ggctttctca taacagcctt gcataaggtt
     9121 gtaaatgcgt ccatgaccaa tggggcgcgc acctgccgtt atcgaaataa gaacgtcgtc
     9181 tcgctttccc ttttttcgat aaggttgagt ttaaacaact ctccagcagg acagtcgcgt
     9241 gtcgtgcctc gtcaaccttg acggcaggta tgattcccag ccactttgaa atgacgtcct
     9301 catcagacga taggaacgac ggaccgagga atattttctc ctcgcttctc caactcgcat
     9361 gctatcatgc tactcattcg gagttcgagg ctgtggtttg ggggagaacc tgagttctcc
     9421 ccttgacagg gaggaaacag gccatgcggt tctcttctag gtttgtcccc gttttggtgg
     9481 tggtattggg gtcgatcgta ggctgcgcgg gctcggatga tgcgactgcg gagccttcgg
     9541 aaatggactc ttccacggat tctcatgaac aagacgagaa tacgacagaa gagggaggac
     9601 aggaaaaccc tgattcgggc acctccctta cggattccgg ctccctcgaa gcagacagcg
     9661 ccgattgcgt cgatgagtgt gaaatagacg gagaacgagc gtgtcatgaa gacgggattc
     9721 gaacgtgtgg ccactacgat accgactcat gcttggaatg gtctccggta gaggcttgtc
     9781 cggaacagca aacgtgcgat ccttcgaccg ttacctgcca ggaagacgaa ggggaaccgc
     9841 tcgctccatt ttcgatcatc ctcatacccg atccccaaaa ctacaccgga ctcaagaatg
     9901 acgcaaacaa tccctatcgt aaacagaccc agtggatagc cgaccattgg gtgagtgacc
     9961 gcatccagac ggtcattcat cttggagaca acacgaacaa caacaagaag agcgaatggg
    10021 ccgtcggaga cgccgcattt gatacgctcg acaaggcggg acgccgttat agcatgacga
    10081 ccggcaatca tgattatcgc gtcgatggcg tgttcaaacg aaatggcagc ctatttgaca
    10141 attactttgg gaagagccgt ttcgaaggca aaccctggta tggcgacagc tacggaaata
    10201 gcaacatcaa taattacacc aaattcgaag tgggaacgat gaagtttttg gtcctaagcc
    10261 tggagttcgc gcctcgcaaa gaggtactcg agtggggcag caaagtcatt ggtaaccacc
    10321 cggatcatca tgtcatcatc gccacccatt gctatttgac acatgacggg aagtacagca
    10381 gttgcaaagc gagtgacaag gttgtcggct cgtccggaga cacgacattc aaagagctag
    10441 ccgctcggca tagcaatgta tttctcgttg tttccgggca tatcaacgat tcggaatacc
    10501 gtatggccaa aggcaataat ggcaacccgg tccatcaaat ggtcgtcgat taccaaatgg
    10561 agaaaccctg cgcggaagcg aaggcagcga attgtaaaaa tcattgtcgc tcgggagggc
    10621 acaccggcaa cggctggctg cgcaaactca tcttcgaccc g
//
LOCUS       DAOSOR010000143        10649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6221_length_10649_cov_4.004814, whole genome shotgun sequence.
ACCESSION   DAOSOR010000143 DAOSOR010000000
VERSION     DAOSOR010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 10649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10649
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6221_length_10649_cov_4.004814"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PK710_09785"
     CDS             complement(<1..343)
                     /locus_tag="PK710_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT10051.1"
                     /translation="MADNVQVLVLDDEPLVGKRLKSVLVKMRCDVEAFDDPMAALARI
                     DEKEFDIVITDVVMGEVDGIQVLERVRGRNPRAKVIIMSAYAMMAMARKAMERGAYDF
                     IAKPFVPEEMRV"
     gene            complement(362..1177)
                     /locus_tag="PK710_09790"
     CDS             complement(362..1177)
                     /locus_tag="PK710_09790"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HOT10052.1"
                     /translation="MSSILVSSQSYAGGPEIARRIAEKLGYRFWSERQLIDEVAKSRA
                     IVPTDLRKALEHPPSLFGLSDVVRRTLVAHLQAVLAAHLLDDSLVFHVPYAHGLIPGV
                     SHILRVRLVAGWASRVAMAEKIEEIPLKKAEKRVAHDDAARNSVSELLFGFTDDDERY
                     DMVVDLDGRQEDAVIEEVVGAMQSHAYDAMTYSMQRLRDVELGFRVRSAVIDLEPSAR
                     VEADHGMVEIRCWAGPSRKAKLEAEVAKRVGGLSGVQGVEVRVFDDSLRRGWA"
     gene            complement(1229..2785)
                     /locus_tag="PK710_09795"
     CDS             complement(1229..2785)
                     /locus_tag="PK710_09795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT10053.1"
                     /translation="MAVETPNRASEESASLSGVLTDPGWNGRAEYEVAADEALRRRPT
                     LTVRNRLMLMLSLFFLLSLAVTIWSIHTLSTVRDSILFLEVADDYTSEIQQARRYEKN
                     YLLYDTNLTDALQHASAAQRLARDNDGKIRKVMGTQAVAAMHQHLDEYHSLMVRIGDT
                     KTPGTRADLEAQIRYHGNEAGEFAKRFVTTERAKVEARLSLAQRVLFASIGGVLLLTV
                     LVANFFARRITSTLRRFAEHTNRIGHGDFTPIMPMRKYRDEFTLLAVAINRMVQELDR
                     RHQILIESHKLRALGTLVAGVAHELNNPLNNMMLTAAILKEQYDELSDEDKLEMVDDL
                     IGQTERSRKIVRNLLDFARESETKLEALDLAEIVDETAHLLGNQLKVKKVKLSVVVPQ
                     GVPPVHGDQQLLNEVLMNLIINALHVVAEGGEIKIVVDPEREDGFVALDVIDNGPGIP
                     NHIIGRIFDPFFTTKPKGTGTGLGLSVARGIVQKLGGSLTVASSPGHGATFSILLPVT
                     GIPSDISSRT"
     gene            complement(2822..3238)
                     /locus_tag="PK710_09800"
     CDS             complement(2822..3238)
                     /locus_tag="PK710_09800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT10054.1"
                     /translation="MQSALRILVVDDEVIACKRLKLSLSSNEYTVECVPGGAEAVQRL
                     ESESFDIVVTDICMDQVDGIAVLDAVSRLHPQSKTIVITGYASVEVAREALAKGAFDF
                     IAKPFQPKDLRVIIDKAAKEIRRVRPKDRADGSSGN"
     gene            complement(3257..4351)
                     /locus_tag="PK710_09805"
     CDS             complement(3257..4351)
                     /locus_tag="PK710_09805"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOT10055.1"
                     /translation="MPPGFIHLDSASAAQVILLGFIGGVLSGFIGSGGAFFMTPGMMN
                     LGVLGPLAVGSNITHKFGKALIGSRKHGELGHVDKKLAIFMLATAAVGIRVAVWIMGH
                     LFHGQGDGGKAQSAGADLYISVVFSVVLAVVSFSMLRDIVRSSGTGDSKPSRKIVDFL
                     TRFRIPPIIHFPVADVRVSLWVVLICGLATGYLAGTIGVGGFIGVPAMIYVFGVPAAV
                     AAGSELYLAVFMGAFGALSYGFAGMVDLRLTLLLYLGSLVGVQIGAYGTKVVKESIIR
                     LATACIIALCVLSRAIAIPVYLRQLGYINYDSAWDGTFQMISKTMLYVSGISGALIIL
                     VFVFRAYFQRRRLRASIVSAGPSRVSEART"
     gene            complement(4396..5913)
                     /locus_tag="PK710_09810"
     CDS             complement(4396..5913)
                     /locus_tag="PK710_09810"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOT10056.1"
                     /translation="MQIICVSRGTYGGGRDLAERLATKLGYPCLAREDLTMAATKAGI
                     PVGKLEMTVVRRRPFNEALAAERDRLIAFITAAILDKALQGGVVYHGRTGHLVLPNLE
                     HILRIRAIMDTDARTELAMARMRIDRDKARRYIEDVDEDRRRWVRLLYNVDWEDPSLY
                     DMVINFDRVSVDNAAAALVAVAQLPEFQRTPAAQRQIEDLMLAAKCRLAIGDDPRTRH
                     IDVKVHADRAKVTVTYPPRHHAAAEVISEVMARVPGVEESTYTMAATNILWIQEKYDA
                     QSPVLSQMVEVAQKWNAAVQLMRLVAAPEQEGLASLPEEDEARPSRALMTTEDNAGIL
                     DDEADDEVGDDGGTGDTLDRLIQEGCAGGITTVRGGSKELLRGMDRTAAVSLVVVGDV
                     FLAKGESVRKRETRELTAHLGESLRIPVIGQDELKEHYLFGAGQWLKMAVFLAMTVVL
                     FVLVFMNQETISWFLVKEGTQHRILAAAVLAIFVPGFAYVWGGATQYLLRLAKFE"
     gene            complement(5915..6343)
                     /locus_tag="PK710_09815"
     CDS             complement(5915..6343)
                     /locus_tag="PK710_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT10057.1"
                     /translation="MLATILVVLDDPKIGEESIRCGLELAKRLASRVEVLVLGEAPGA
                     TTAELDGFAALAQVARDEGVDLSIHRRSGDKTSELLKYLASQAHVRALVWGGDDAALS
                     VTSSSRRRHWFGRMREQVECPIVTASRKRSIAKRSEGSEN"
     gene            complement(6707..8080)
                     /locus_tag="PK710_09820"
     CDS             complement(6707..8080)
                     /locus_tag="PK710_09820"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOT10058.1"
                     /translation="MNDRDAHESAGRVLVVEDESVARKMLTRILTRDGYEVSEASGGE
                     QAVRTLGMQRFDVVLTDLVMQGVDGLRVLREARRLDDGVEVILMTGHATVDTAIEAMK
                     GGAFHYLQKPLRPDEVRSLVRQACEKKRLRARLQAFEQEGGRGGLQRIVGTSAAMKAI
                     REQIEQIRASDSNVLITGESGTGKELVARAIHATSRRSKGKFVAFNCASFADELLANE
                     LFGHEREAFTGASSVRAGLLESGDGGTVFLDEVGDMTPAMQAKMLRVVQEREILRVGG
                     TRPLPVDIRIISATNKDLPRLIGLKLFREDLFFRLNVIPLRMPPLSERREDIPLLAMH
                     FLRRLEPTVGKHLEGFTDAALGMLIDYHYPGNVRELENIVERAATLAVGERIDVANLP
                     PDMTAMEVRAFRFDTEPIRTLEQVERDYIQWVLNRVGHNKTQAARLLGIDRVSLYRKL
                     RKEQLLD"
     gene            complement(8189..9424)
                     /locus_tag="PK710_09825"
     CDS             complement(8189..9424)
                     /locus_tag="PK710_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10059.1"
                     /translation="MPSSFRHPQRFLKLFAYLTLVGATACSGYEPPCSTEPSTWIVDE
                     SLEEQAPFFPSLEPSDRGTLVASWLMTPLAQTSHAGALRDSVVGVAILSQEGRLLERY
                     AFPLFEGWEDDDLPHDNFGFCFSPYGLVLRWTRMSTLSAVGDPPRIRTSLWVQRIFRN
                     GRNEPPYSPANMNCEQCVVQLSGACRDGFASVLFSALPEPSQGSADEPEILVFSWDLA
                     ATRSTSGPLAWLDATPTQASAPYLLGDEKSLWLLTQGQGWPIDAEFRKRAGPIPLPLS
                     NQRFVHFSPSKSETWMTWSVASDGKDQQDVFFRRFDKNGVPSTPLARLGEASFLASVA
                     RSQGELGAVLRNDGDDYFAWADDRGVRMGGDVPLGPADQVLSGGIRREWLMALGGGRF
                     VRLQSFPNRLQRTEIWCER"
     gene            complement(9462..10340)
                     /locus_tag="PK710_09830"
     CDS             complement(9462..10340)
                     /locus_tag="PK710_09830"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HOT10060.1"
                     /translation="MDRIDLLASIPMFEGLENEDLEVLARELRVHPLVPGDMVFHAGD
                     EGNSMFIVAAGVVDIFLPGLDPSSNLVVATLEAGTYFGEFALFDNQPRSASAQVTSDT
                     VLLELGRDALMRYWEDRPNAALGILRTMSERLRETNAMLGARATRNVVEEYEQGMRWS
                     DRVADQVARLNGSWSFIFVLSAVMVIWALLNVHHLLFGHPPDPYPYIFFNLVLAVLIA
                     LQWPLIVMSQNRRAEKERRAAELDFQVNLKNETHCEELLGELRAFRAEWEGREEQLAG
                     LLARRHLGDSYEARDR"
BASE COUNT         2365 a         3463 c         2687 g         2134 t
ORIGIN      
        1 tcactctcat ttcttctggc acaaaaggct tggcgatgaa atcgtacgcc cctcgttcca
       61 tggcttttcg cgccatcgcc atcatcgcat acgcgctcat gataatcact ttggctcgcg
      121 gattccgccc gcgcacccgc tcgagcacct gaatgccatc cacctccccc atcaccacat
      181 ccgtgatcac gatatcgaat tccttctcat cgatacgcgc cagcgcagcc atcgggtcgt
      241 cgaaagcttc gacgtcgcac ctcattttga cgagcaccga cttgagtcgc ttgcccacca
      301 aaggttcgtc gtcgagcacg aggacctgca cgttgtctgc catctcacac ctcctgctgt
      361 ctcatgccca gccccttcgc agcgaatcat cgaatacgcg cacttccacg ccctgcaccc
      421 cggatagccc gcccactcgt ttggcaacct ccgcttccaa cttggctttg cgcgatggac
      481 cagcccaaca acgaatctcc accatcccat ggtcagcctc cacccgagcg cttggctcca
      541 gatcgatgac cgcactgcgc acacggaatc ctagttccac atcccgtaat cgctgcatcg
      601 aatacgtcat cgcgtcgtac gcatggctct gcatcgcccc caccacttcc tcgatcacgg
      661 catcttcttg ccgaccatcg agatccacca ccatgtcgta ccgctcgtcg tcgtcggtga
      721 atccgaacaa cagttccgaa acgctatttc gcgcggcatc gtcatgggcg acccgctttt
      781 ctgccttctt tagcggaatt tcctcaattt tctccgccat cgccacgcgc gatgcccagc
      841 cagccaccaa acgcacacgc aggatgtgcg agacgcccgg aatcaatcca tgcgcataag
      901 gaacgtgaaa taccaggctg tcgtcgagca aatgcgccgc gagcaccgct tgcagatgcg
      961 ccaccagcgt gcgtcgaacc acgtcggata gcccaaacag cgagggtgga tgctcgaggg
     1021 cttttcgtag gtccgtcggc acgatggcac gggattttgc cacctcatcg atgagttgcc
     1081 gttcgctcca aaaccgatac ccgagttttt ccgcgatcct tcgagcgatc tcggggcccc
     1141 ccgcgtagct ctgactcgaa accaagatgc tcgacatgac gaatttcctt tcctccatgc
     1201 aaggagcccg cgaaacatgc cccttggatt acgttcgcga cgagatatcc gaaggaatcc
     1261 cggtaaccgg cagcagaatc gaaaacgtgg caccgtgccc tggtgaactc gccaccgtca
     1321 aggaaccccc aagtttttgc acgatacccc gcgccacgga tagcccaagc cccgtgcccg
     1381 tgcctttggg cttggtggta aaaaatgggt cgaatatgcg cccaatgatg tgattcggaa
     1441 tgcccggacc gttgtcgatg acgtccaacg ccacgaaccc atcctcgcgc tccggatcca
     1501 ccacgatttt gatctcgcct ccttcggcaa ccacgtgaag ggcattgatg atcaagttca
     1561 tgagcacctc gttgagaagc tgctgatcac catgcacggg cggaactcct tgcggaacca
     1621 ccacggagag cttgaccttc ttgaccttga gttggttacc gagcaagtga gcggtttcat
     1681 caacgatttc agcgagatcc agcgcttcga gcttggtttc gctctcgcgc gcgaagtcca
     1741 ggagattacg cacgattttt cgcgaacgct cggtctgacc gatgagatcg tcgaccatct
     1801 cgagcttgtc ctcatcggaa agctcatcgt attgctcttt gagaatggcc gccgtgagca
     1861 tcatgttgtt cagcgggttg ttcaattcat gagccacccc cgcgaccaag gtgccgagag
     1921 cacgtaactt atgagattca ataaggattt gatgacgacg gtcgagttct tgcaccatgc
     1981 ggttgatagc caccgccagc agcgtgaatt cgtcgcggta cttacgcatc ggcatgatcg
     2041 gcgtaaaatc gccgtggcca atgcggttcg tatgctcggc gaagcgacga agggtgctcg
     2101 tgatacgcct ggcgaaaaaa ttcgccacga gcaccgtgag aagcaaaacg ccgccgatgg
     2161 aagcaaacaa aacacgctgc gcaagagata atcgcgcttc gaccttcgcg cgttccgtcg
     2221 tcacgaaccg tttggcgaat tcaccggctt cgttgccgtg atagcgaatc tgggcctcga
     2281 gatccgcgcg cgttcccggc gttttcgtat ccccgatgcg aaccatcaag ctgtgatatt
     2341 cgtcgagatg ttgatgcatc gcggccacgg cctgcgttcc catcaccttg cggattttgc
     2401 catcgttgtc gcgggcgagc ctctgcgccg cactggcgtg ctgcaaagca tccgtcaaat
     2461 tcgtatcgta gagcaggtag tttttctcat accgacgcgc ttgctggatt tcgctcgtgt
     2521 aatcatcggc gacttccaaa aataagatgc tgtcgcggac ggtcgaaagc gtatgaatgg
     2581 accagatcgt gaccgccagg ctcagcagga aaaaaagaga aagcatgagc atcaggcgat
     2641 tgcggacggt cagcgtgggt ctacgtcgca aggcttcatc cgcggcgact tcatactcgg
     2701 cccgtccatt ccaccccgga tccgtaagca cgccgctcag gctggctgat tcctcactcg
     2761 ctcgatttgg cgtttcgact gccacattcg ctcccaccgg gccgtctgac ccttggctga
     2821 cctagttgcc gctggagccg tcggcacgat ccttgggccg tacgcgacga atctccttgg
     2881 ctgctttgtc gatgatgaca cggaggtcct tgggttgaaa cggttttgcg ataaagtcga
     2941 acgctccttt ggccaacgcc tcccgggcga cttcgacact cgcgtagccc gtaatcacaa
     3001 tggttttcga ctggggatgc agacggctga ccgcgtcgag cacggcaatg ccatcaacct
     3061 gatccatgca aatatccgtc acgacgatgt cgaagctctc ggattcgagc cgctggaccg
     3121 cttcggcccc gccaggcaca cactcgaccg tatactcgtt gctcgacagc gagagcttaa
     3181 ggcgcttgca ggcgataacc tcatcgtcca ccacgagaat ccgaagagcg ctttgcatga
     3241 gtcaaccttt cctttctcac gttcgggcct ccgacaccct gctgggacct gcggatacga
     3301 tggaggcacg aagccttcta cgctgaaaat aggctcgaaa aacgaagacg aggatgatga
     3361 gcgcaccgct gatgccactc acgtatagca tcgtcttgct gatcatctgg aaggttccat
     3421 cccaagctga atcgtagtta atataaccga gttggcgtag atacaccgga atggcaatgg
     3481 ctcggctgag gacgcatagc gcgatgatgc atgcggtagc caatcggatg atcgactctt
     3541 tcacgacttt ggtgccataa gcgccaatct gcacccccac gagcgaaccg agatacagaa
     3601 ggagcgtgag gcgcaggtcg accataccgg cgaagccgta gctgagagcg ccgaaggctc
     3661 ccatgaacac cgcgagatat agctccgagc ccgccgccac ggccgcggga accccaaata
     3721 cgtagatcat ggccggaaca ccgatgaatc cgcccacgcc gatggtgccc gccaagtagc
     3781 ctgtcgcaag accgcaaatg agcaccaccc acaacgatac ccgcacgtcc gcgacaggaa
     3841 aatggatgat gggagggatg cgaaagcgtg taagaaagtc cacgattttg cgggatggct
     3901 tgctgtcacc ggtgcccgaa ctgcgaacaa tatcgcgcaa catggaaaac gatacgaccg
     3961 cgaggacgac cgagaagacg acactgatat ataagtccgc gccggcgctc tgcgccttac
     4021 cgccgtcgcc ttgaccgtga aatagatgcc ccatgatcca taccgccaca cgaattccca
     4081 ccgcggcggt agcgagcatg aaaatggcta gttttttatc gacgtggccc agctccccat
     4141 gcttgcggga gccaatcaag gctttgccga acttatgggt gatattgctt cccaccgcga
     4201 gggggcccaa aacacccagg ttcatcatgc ctggagtcat gaaaaatgct cccccactgc
     4261 caatgaaacc actgagcaca cccccgatga atcccagcaa aatcacttgc gccgcggagg
     4321 cgctgtcgag atgaatgaac cctggcggca tggtcctact cccgtttctt cggttgaaac
     4381 ctcagcccac gatggctatt cgaacttggc aagcctcaac aaatactgcg ttgccccgcc
     4441 ccatacatac gcgaagccgg gcacgaagat cgccaacacc gccgcggcca aaatgcggtg
     4501 ttgcgttcct tccttgacca aaaaccacga gatcgtctcc tggttcatga ataccaatac
     4561 gaacaaaacc acggtcatcg ctaaaaacac agccattttc agccattgcc ccgcgccgaa
     4621 aagataatgc tccttcaatt cgtcttgacc aatcaccggt atccgcagtg attcgcccaa
     4681 atgcgccgta agctcgcgcg tctcgcgctt ccgcaccgac tcgcctttgg ccaaaaagac
     4741 atcacccacc accaccaaac tcaccgccgc ggtccgatcc atgccccgca gcaattcctt
     4801 cgatccaccc cgaaccgtcg tgatccctcc cgcacacccc tcctgaatca gccggtccaa
     4861 cgtgtcgccc gtaccaccgt catcgcccac ctcgtcatcc gcctcatcgt cgagaatccc
     4921 cgcgttgtcc tccgtcgtca tcaacgcccg cgaaggcctc gcctcatcct cctccggcaa
     4981 cgaagccaat ccctcttgct caggagccgc taccaaccgc atgagctgaa ccgccgcatt
     5041 ccacttctgc gctacctcca ccatctgcga aagcaccggc gattgggcat cgtacttctc
     5101 ctgaatccac aaaatattcg tcgccgccat cgtataggtc gactcctcga cccccggcac
     5161 acgcgccatc acctcggaaa tcacttcggc cgctgcatga tgacgcggcg gatacgtcac
     5221 cgtcaccttg gcccggtccg catggacctt gacatcgata tggcgcgtac gggggtcatc
     5281 gccaatcgcc aaccgacact tggccgccag catcaggtcc tcgatctgcc gctgcgccgc
     5341 aggtgtacgc tgaaattcgg gaagctgggc caccgccacg agcgccgccg ccgcgttatc
     5401 cacactcaca cggtcgaagt tgatcaccat gtcataaagc gatggatcct cccaatccac
     5461 gttgtaaagc aatcgcaccc aacgccgacg atcctcgtcc acgtcttcga tatagcgacg
     5521 cgccttatcc cgatcgatac gcatgcgcgc catggccagc tcggtgcgcg catcggtatc
     5581 catgatggcg cggatacgta gaatatgctc taaattaggc aatacgaggt ggcccgtacg
     5641 cccgtggtac accacccccc cttgcagggc tttgtcgaga atggccgcgg tgatgaaggc
     5701 gatgagacgg tcccgttccg cggccaaagc ctcattgaat ggccgtcgac gcaccacggt
     5761 catctcgagt ttgcccaccg gaatgcccgc cttggtggcc gccatggtca ggtcttcccg
     5821 cgccagacag ggatagccca acttcgtcgc aagcctttcg gcaaggtctc gcccaccacc
     5881 ataagttccc ctggaaacgc agatgatttg catgtcaatt ctcgcttcct tcgctccgtt
     5941 tcgcgatcga acgcttccga gaagctgtca cgataggaca ttcgacctgc tcgcgcatgc
     6001 ggccaaacca gtgacgacgt cgactgctgc tcgttaccga caaggccgca tcgtcaccgc
     6061 cccaaaccaa cgcgcggaca tgcgcctggc ttgccaaata ctttagtaat tccgatgtct
     6121 tatctccact gcgacgatgg attgaaaggt ccactccctc gtcccgagct acctgagcca
     6181 acgccgcaaa accatccaat tccgcagtcg tcgcccctgg tgcctccccc agcaccagca
     6241 cctccaccct gctcgccaat cgtttggcca gctccagccc acaacgaatg ctctcttcac
     6301 cgattttcgg gtcgtcgagg actaccagga tggttgccaa catgaaacta tccagccacg
     6361 gacagggaac tggtctctgc ccacgcgtca ataagccgat gcttcttcaa aaaagcgtcg
     6421 gtcagaggaa aacgagtctc cgccaaagac ctcgcggcac ccgtttcgat ggattggcgc
     6481 gtggtagagc atgtcccgtg ccacccgtaa ccggcagcca atgcacgcaa aaatcgtcca
     6541 ccagagggat agagccacac tccagcctgt tgcgatttgc cacaggcgaa ctcccgaacg
     6601 acatatttat ggtgtaattt tgactattta tgccctatat ggcaattggt tacaggggtg
     6661 taacaaaatg caacgtcgtt tggattgctt ttcccccctg tcatcgtcaa tcgagcaact
     6721 gctccttcct cagcttccga tagagcgata ccctgtcaat acccaatagc cttgccgcct
     6781 gtgttttgtt atgccccacc cgattgagca cccattggat ataatcgcgc tccacctgct
     6841 cgagggtcct gatcggctcg gtgtcgaatc gaaacgcccg cacctccatg gcggtcatgt
     6901 ccggcggaag attcgcgaca tcgatgcgtt cgcccaccgc aagggtagcc gcgcgctcca
     6961 cgatattctc gagttctcgc acgttgcccg gataatgata gtcgatcaac atacccaacg
     7021 cggcatccgt aaacccttcg agatgcttgc ccaccgtggg ctcgagccgt cgcagaaagt
     7081 gcatggccag cagcggtata tcctcccgcc gttcggataa ggggggcatg cgcaggggaa
     7141 tcacgttcag gcgaaaaaac aagtcttcac ggaatagttt cagcccgatc agccgcggca
     7201 aatccttgtt ggtcgccgaa atgattcgga tatccaccgg caggggtctg gttcccccca
     7261 cccgcaggat ttctcgttcc tgcaccaccc gcagcatttt cgcctgcatc gctggcgtca
     7321 tatcccccac ctcatcgaga aataccgtgc cgccatcgcc gctttccaac aacccggccc
     7381 gcacactgga agcgcccgtg aacgcttccc gctcgtgccc gaaaagttcg ttggccaata
     7441 actcatcggc aaacgacgcg cagttgaaag ccacgaactt ccctttgctc cttcggctcg
     7501 tcgcatgaat cgcccgcgct accaattctt ttcccgtacc agattccccg gtaatcagaa
     7561 cattcgaatc cgaagccctg atttgttcga tttgctcgcg tatcgccttc atcgccgcgc
     7621 tcgtaccgac gatccgctgc aacccaccgc gtccgccttc ctgttcgaaa gcttggagcc
     7681 gcgcgcgaag acgctttttt tcgcaggctt gacgcaccag actgcgcacc tcgtccggac
     7741 gcaaaggctt ttgaagatag tggaaggcac cgcctttcat cgcttcgatc gccgtgtcca
     7801 ccgtggcatg gcccgtcatc aagatcacct cgaccccatc atccaggcga cgcgcttccc
     7861 gcagcacccg caacccgtcc accccctgca tcaccaaatc cgtaagcacc acatcgaaac
     7921 gctgcattcc aagcgttcgc accgcttgct cccccccgga ggcctccgaa acctcatagc
     7981 catctcgcgt gagaatccgc gtcagcatct ttctcgcgac cgattcgtct tccactacga
     8041 gaacgcggcc tgccgattcg tgcgcatcac ggtcgttcat ccgtttttca cctccaagac
     8101 gccatgccgc ggcgccgata caagcctatc ttgccacgaa aacgaggaat attcgccagc
     8161 agcttttccc tcccctcgtt ccccatcctc atcgctcaca ccaaatctcc gtccgctgca
     8221 agcgattggg aaacgactgg agacgtacga atcgcccacc gcccagcgcc ataagccatt
     8281 ctcggcgtat tccccccgaa agaacctggt ccgcggggcc caggggcacg tcgccgccca
     8341 tgcgaacgcc ccgatcgtcc gcccacgcaa aatagtcgtc gccatcgttt cgtaatacgg
     8401 ctcccaattc tccttggctc cgcgccaccg aggcaaggaa agaagcctcg ccaagccgtg
     8461 ccaacggcgt cgacggcacg ccgtttttgt cgaatcgacg aaaaaaaaca tcttgttggt
     8521 cttttccgtc ggatgccacc gaccacgtca tccaggtttc ggatttcgat ggcgaaaagt
     8581 ggacgaagcg ctgattcgac aggggcaagg gaatcggacc cgcgcgtttt cgaaattcgg
     8641 catcaatggg ccacccctgc ccttgagtca acaaccacag actcttttcg tcccccagca
     8701 agtacggcgc cgacgcctgc gtgggggtgg cgtcgagcca ggcaagcggt ccggaggtac
     8761 ttctggtcgc agccaaatcc catgaaaaaa caagtatttc cggctcatcc gcgctcccct
     8821 gagacggctc gggcagggcc gagaacaata ccgacgcaaa gccatcacga caagcaccgg
     8881 aaagctgaac gacacattgt tcacaattca tattggccgg tgaatacggc ggttcatttc
     8941 gaccgtttcg gaaaattcgc tgcacccaca agctcgttcg aatacgaggg ggatcgccga
     9001 ccgcactgag cgttgacatt cgcgtccacc gaagcacgag cccgtaaggg gaaaaacaga
     9061 aaccgaaatt gtcgtgcggc agatcgtcgt cttcccaccc ctcgaaaagc ggaaatgcgt
     9121 atcgctccag caagcggcct tcctgggata ggatggctac ccccaccacg ctgtcgcgca
     9181 atgcccccgc gtgcgatgtc tgggccaaag gagtcatgag ccaggatgcc acgagggtcc
     9241 cgcgatcgct cggctcgagg gaaggaaaaa agggagcttg ctcctccaac gattcatcca
     9301 cgatccatgt ggatggttcg gtggaacagg gcggttcata tccgctgcag gcggtggctc
     9361 cgacaagagt caagtacgcg aataatttca gaaatctttg tggatggcga aaggaagagg
     9421 gcatcgggag cctcagacgc tgggggggcg atgggggtgc tctatcggtc acgcgcctcg
     9481 taagaatcgc caaggtgtcg tcgcgccagc aagccagcaa gttgctcctc tcgcccttcc
     9541 cattcggcgc gaaaagcacg gagttctccc aacaactcct cgcaatgggt ttcgtttttc
     9601 aagttcactt ggaagtcgag ttccgccgcg cgcctttcct tttcggcgcg acggttctga
     9661 ctcatgacga tgagaggcca ctgcaaggcg atgagaaccg ccagcaccaa gttgaaaaag
     9721 atgtaaggat aagggtccgg agggtgtccg aaaagcaagt gatgcacgtt gagcagcgcc
     9781 cagatgacca tcaccgccga caatacaaaa atgaatgacc acgagccatt gagccgcgct
     9841 acctgatcgg ccactcgatc cgaccaacgc atgccttgct cgtattcttc gacgacgtta
     9901 cgcgtcgccc gcgcaccaag catcgcattg gtttctcgta accgctcgga catggtgcgc
     9961 agaattccca acgctgcgtt aggacgatcc tcccaatatc tcatgagcgc gtcgcggcca
    10021 agctccagca acacggtgtc ggaggtgacc tgcgcgctcg cgcttcgagg ctgattatcg
    10081 aataaagcaa actctccgaa atacgtgccc gcttccagcg ttgccacaac caggttcgaa
    10141 gaaggatcca agccgggaag aaaaatatcc accactcccg cggcaacgat gaacatcgag
    10201 tttccctcgt cgcccgcgtg aaataccatg tcgccaggca cgagcgggtg gacccgaagc
    10261 tcgcgcgcca acacctccag gtcctcgttt tcgagccctt cgaacatagg tatgctggca
    10321 agcaaatcga tccggtccaa gatgctgaat tgtcacaggt tgagcgccga gatgcaatca
    10381 gaaccggcac atctttgaga atccgatggc cagggcggtc ggaatgacgg aaactcgcgc
    10441 gcgataaccg cgttttccaa gggtgcatcg agggtcgagg agcataagag ggctacggac
    10501 gccagccaat caacccgcaa ccgtggaaat cgctaagcca ccaaagactc cgatacgcca
    10561 ccaggctcag cttgagcgct tcgcaccctg gttttcctat cctctggagg gctaacagat
    10621 cccctcattt tttcctgaat tggaaaggg
//
LOCUS       DAOSOR010000144        10591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6249_length_10591_cov_3.804860, whole genome shotgun sequence.
ACCESSION   DAOSOR010000144 DAOSOR010000000
VERSION     DAOSOR010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 10591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10591
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6249_length_10591_cov_3.804860"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PK710_09835"
     CDS             <1..444
                     /locus_tag="PK710_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10061.1"
                     /translation="YNASVTSLLHAGWLLAWSLVRTLLRRILLPNRDGLVEFTRHYAN
                     DRLMPLTISERDLIPRLSACIGCGRCDWYQTQHTVASRSAYRGMMAFVLSASRSMPDF
                     DVAADAMANLSEEQLSTLRQRCPVDIPFSELAGFVRSKAQLMLSP"
     gene            441..>1067
                     /locus_tag="PK710_09840"
     CDS             441..>1067
                     /locus_tag="PK710_09840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT10062.1"
                     /translation="MNGEPTSSQDPWALVLGASSGFGAATSFALAKAGFHIVGVHLDR
                     RATLQGADEVAANVRSVHRHAWMFNTNAADESKRNEVLDEVTKRWSRFDRIPKFTVLF
                     HSLAFGALLPHLSDDPQNRLSLRQIEMTSLVMAHSLVLWTQALIEREMMVEGGRIYAM
                     TSTGANTVWKGYGAVSAAKTALEAHVRQLAVELAPLRITVNALCAGVTD"
     assembly_gap    1068..1077
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1078..1258
                     /locus_tag="PK710_09845"
     CDS             <1078..1258
                     /locus_tag="PK710_09845"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT10063.1"
                     /translation="LKKIPDHERMIEIALRKNPHGRLTQPQDVAKAVVALCVPETYWI
                     TGNVIHIDGGEFIVG"
     gene            complement(1263..3560)
                     /locus_tag="PK710_09850"
     CDS             complement(1263..3560)
                     /locus_tag="PK710_09850"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOT10064.1"
                     /translation="MRAFQIVIDRRSTHSASRMFSNLRGVLDVLVDGTNVTARIGQDH
                     AIPLLRDLAYAAADLAIHRHTRATVRFYDQRDAWALGLERVDNDVLVTVFQAGEHPHV
                     VVQERRVLGISLLRGIMNAIESVLDQEKDKTEPLFRDLDAARRFLESLRWQTSSRSIE
                     IVDVVVEAQSDAAVSFSTEFSLRVYDDEPVLDNVERNELHSLLFTGPFHVRIGDVRRD
                     FGNVHPFLIAECLLREAVMVLQAWESGSAFNHRRQVSDILIGIRLTTQGILSLTLGGA
                     SSAGFHGTLTFPSISVVSFVEATLAFSRGLVRPLLRHDRSQRSNLRMRVFREDVRSLS
                     TALREVVRQDAKLNRSPESYRAFAASVATRPASNHSWGHGRLRFHANWQAAVPGVDLQ
                     SVFLFGDRLVAAGSRELASIDKQSGRILWRVPIERGVSVAAPHGIVRLSPRGFMHFHD
                     LTTGEISMVVRLAARNGGLPAGAIVNVPGLPRLLIVSEGEKHITAVDYVSGEVRWRHT
                     LGRGRSIKVRRAGKLLVVTSSDQTLTALDVSTGETVWRVRDKLRFTRPVSYDRNDLFA
                     VSGDGALVQSGIEKLHAFDPWTGETKWIQCLDAGIRSMGAPLTTDELVVVALHHRRGL
                     GLCAFDRNDGEPRWRLEPGFAPTISAWLLVDDLIAINCENGMMLGLDWRTGTIRWRHT
                     TESPPDGDAPRNLDPVLRSGALFLPQRNVIVVRPTDGERLGEVEADLVPDLIRVDDAC
                     NVVVVEESGHVAAFSAGARLTLIKN"
     gene            3670..5121
                     /locus_tag="PK710_09855"
     CDS             3670..5121
                     /locus_tag="PK710_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10065.1"
                     /translation="MQRFWASLLGLSLACGVAFALVGFTVDDALISARYASHLAQGVG
                     HRFNGSGPLTDGVTPLVWPYLLCCFGSDPLRLFAVARWVGMIGWLGTSCFLGMHLARV
                     VGPRWSLGHLSWVVAFGTPAVGAWASAGMETSLALCLVTVAAVMGDRDKRKTASVLVG
                     VSCLLRPELLPFALTLGLGSAWLDERFHQQRTVRSVRSLLIHGSLVMVPFLLVMLIRW
                     KMFDAVAPLALRAKPSDITHGIRYALATWLIAGPPLSCMAPWAFRKLRGWPAWLLAGW
                     LVHTVCCVLVGGDWMPLSRLFVPVLPALAIVFAHLASVAHVASTIARALVCMMGQAFV
                     YATVGVGAHHVVRDRMVLIEQLRGQLRPDDVVACLDVGWVGVAHRGSVFDLAGVTDPQ
                     VAALPGGHTSKRVTAEMLDARGVTHLLVLLARGESGLDAWQSCGLGRSVEVRLCRSEA
                     VRAKFHPTRLVRSTDTLQYLLLERGSSSGRGQR"
     gene            complement(5098..5880)
                     /gene="tsaA"
                     /locus_tag="PK710_09860"
     CDS             complement(5098..5880)
                     /gene="tsaA"
                     /locus_tag="PK710_09860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOT10066.1"
                     /translation="MAPISLNPIGIAHSPFHTSHEVPRHGAPATIEIFPAYRNALDGI
                     DSCSHIWIIGFFHNAARDLLQARPRKSSPSSPPRGVFSMRSPVRPNPLGLTCARLLER
                     QGSTLLLDRLDFQDKTPVVDIKPYSPGWDLILSASGIHRYDPSRYSEEELLLALQHDA
                     ANALGPSHPSPARSAIIQALASLILHHVDIRSPSTHFAIPIPDERVDLLMGSTAASFG
                     NQRLHILSLPDSVLLRSWTPRIAFDVSICSGSPQLLPLSPPG"
     gene            complement(5913..5987)
                     /locus_tag="PK710_09865"
     tRNA            complement(5913..5987)
                     /locus_tag="PK710_09865"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5952..5954),aa:Glu,seq:ctc)
     gene            complement(6023..6093)
                     /locus_tag="PK710_09870"
     tRNA            complement(6023..6093)
                     /locus_tag="PK710_09870"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6059..6061),aa:Gln,seq:ctg)
     gene            6347..6637
                     /gene="groES"
                     /locus_tag="PK710_09875"
     CDS             6347..6637
                     /gene="groES"
                     /locus_tag="PK710_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOT10067.1"
                     /translation="MKLRPLGDRVVIKRVEQEEKTKGGIIIPDTAKEKPVEAEVIAVG
                     PGKALKDGKIRPIEVKQGDRVLIGKWSGTEIKLEGIEHVIVREEDILAVVSR"
     gene            6656..8311
                     /gene="groL"
                     /locus_tag="PK710_09880"
     CDS             6656..8311
                     /gene="groL"
                     /locus_tag="PK710_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735365.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOT10068.1"
                     /translation="MAAKEIKYNRAARAAMLRGVDGLANTVKVTLGPRGRNVVLEKSW
                     GSPVITKDGVTVAKEIEFGDKFENMGAQMVREVASKTSDKAGDGTTTATVLAQAIFVE
                     GIKLVEAGVNPMDLKRGIDAAVEKVVDAVKGMSHATKDRNQIAQVATISANGDTTIGT
                     TIADAMEKVGKEGVITVEENKGTETELDVVEGMQFDRGYVSPYFVTDAEKMEAVLEEA
                     YVLVHEKKISNMNDLLPLLEAVVREGRPLLILAEDIENEALATLVVNKLRGTVKVCAV
                     KAPGFGDRRKEMLKDIAILTGANPFMEDLGLKLEKATVRDLGRAKRIVVDKDNTTIVE
                     GYGDKKEIKGRTEAIRKQIEDTTSDYDREKLQERLAKLAGGVAVVRVGAHTETEMKEK
                     KARVEDALNATRAAVEEGIVPGGGVALVRARTALDTLNFNDERRHGVAIIRRAIEEPL
                     RQIATNAGVEGAVVLSKVAEGKDAFGFNAATEVFENLLEAGVLDPAKVVRSALENAAS
                     VAGLMLVTEAAIAEKPKKPVKDAGGAGGMGGMGGMGGMGGFDM"
     gene            8625..9269
                     /locus_tag="PK710_09885"
     CDS             8625..9269
                     /locus_tag="PK710_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOT10069.1"
                     /translation="MSDTLFEKFGREFEEGFVLFQEGQPGNEMFVVQSGVIRLTKRVG
                     REDKPLAFVGAGEFLGEMAILNAKPRTATATVAEGPARCLVIDAQTLEVMVKKNAEIA
                     LRLIKKLAKRLDSADALIEILMHRDPKARVLLALVRHAEDYGEATDQGMVLRSTGQEL
                     GEQVGVDGQVVEQVMARLRRLRLIQEMEDGRQVVPDLSRLEEFIEFLDQPTPGG"
BASE COUNT         2301 a         2774 c         3229 g         2277 t
ORIGIN      
        1 tacaatgcaa gcgtgacttc ccttcttcac gccggttggt tgttggcatg gtccctcgtg
       61 cgaaccctgc tccgtcgcat cctattaccg aatcgtgatg gcctggtcga attcactcgt
      121 cattatgcca atgaccggct catgcccctg acgatttccg aacgagatct aatccctcgt
      181 ctgtccgcat gcattggttg cggaagatgc gattggtacc agacgcagca cacggtcgct
      241 tcaagaagcg cttatcgagg tatgatggct ttcgtgctct ccgcttcgcg atccatgcct
      301 gatttcgatg tggccgcgga tgccatggcc aacctatcgg aagagcaatt gtcgaccctt
      361 cgacagcggt gccccgtgga tattcctttt tccgagcttg ctggcttcgt gcgaagcaaa
      421 gcgcaactca tgttgtcgcc atgaacggcg aacctacctc ttcccaagac ccttgggctc
      481 ttgtacttgg tgcctcgagc ggttttggcg ccgcaaccag cttcgcgctc gcaaaagctg
      541 gtttccatat cgtaggcgtt cacttggacc gtcgcgccac cctgcagggt gccgatgagg
      601 ttgccgcaaa cgttcgttcc gtccaccgtc acgcttggat gttcaacacc aatgctgccg
      661 acgaatccaa acgaaacgaa gtgctcgatg aagtgaccaa gcgctggagc cgcttcgaca
      721 ggatacccaa atttactgtg ttatttcatt cacttgcgtt tggggcctta cttcctcacc
      781 tttccgatga tcctcaaaac cgtctgtctc tccgtcaaat cgaaatgacc agcttggtca
      841 tggctcactc cctcgtttta tggacacaag ctttgatcga acgggaaatg atggtggaag
      901 gaggacggat ctatgccatg accagcaccg gggccaatac cgtttggaaa gggtatggtg
      961 ccgtcagtgc ggcaaaaacg gctcttgaag ctcatgtgcg gcaactcgct gtcgaactcg
     1021 cccctctacg catcaccgtg aatgcgttgt gtgcgggcgt tacggacnnn nnnnnnngct
     1081 caaaaaaatt ccggaccatg aacgaatgat cgagattgcg ttacgaaaaa atcctcatgg
     1141 caggttgacc caaccacagg atgtagcaaa ggccgtggta gctctttgtg taccggaaac
     1201 ctattggatt accggcaatg tcattcatat cgatggagga gagttcatcg taggataggg
     1261 gattagtttt tgatcagcgt gagacgggcg ccggcagaga aggccgcaac gtggccactt
     1321 tcttcaacga cgaccacatt gcacgcatcg tccacccgga tgaggtcggg aacgagatcc
     1381 gcttcgactt cgcccagtct ctcaccatcg gtcgggcgaa cgacgatcac atttcgttgt
     1441 ggtagaaaca gcgcgccaga tcgcaggacg ggatcgagat ttcgaggcgc atcaccatcc
     1501 ggaggggatt ccgtggtgtg tcgccaccgg atggttcccg tccgccaatc cagaccgagc
     1561 atcatcccgt tttcgcaatt gatggcgatg agatcatcga ccaggagcca cgcggaaatg
     1621 gtaggcgcaa aacctggttc gagccgccat cgaggttcgc catcgttgcg atcgaaggcg
     1681 cataggccca acccacgacg atgatggagg gctacaacga cgagttcgtc agtggtcaat
     1741 ggcgctccca tcgagcggat cccggcgtca aggcattgaa tccacttggt ttctcctgtc
     1801 caaggatcga aagcgtgcag cttttcgatt ccgctttgaa cgagcgcgcc atcacccgaa
     1861 acggcaaata gatcgtttcg gtcataggaa acgggccttg tgaaacgcag tttatctcga
     1921 acgcgccata ccgtctcgcc cgtggatacg tcgagagcag tcaaagtctg gtccgacgat
     1981 gtgaccacga gcagtttccc tgctcggcgc actttgatcg agcgtcctct acccagggtg
     2041 tggcgccacc tcacttcacc ggatacataa tcaaccgcgg tgatgtgttt ttctccttcg
     2101 ctgacgatga gtaatctggg cagaccgggt acgttgacga tggctccggc gggtaagcct
     2161 ccgtttcgtg cggcaagacg caccaccatc gagatttcgc ccgtcgttag gtcatggaag
     2221 tgcatgaagc cacgaggcga caggcggacg ataccgtgag gagcagcgac ggaaacgcct
     2281 cgttcgatgg gaactcgcca aagaattctt ccgctttgtt tgtcgatgga cgccagttct
     2341 cgagatccgg cggcgacgag tcggtctccg aatagaaaca cggattgcag gtcgacaccc
     2401 ggtacggcgg cttgccagtt cgcatgaaaa cgaagacgtc cgtgtcccca ggaatggttc
     2461 gaggctgggc gtgtggcaac agaagccgcg aaggcgcggt agctttctgg ggatcgattg
     2521 agtttcgcat cttgccgaac gacttctcgc agcgccgtag acagagaacg cacatcttcg
     2581 cggaaaacac gcatccgaag attgcttcgt tgggagcggt cgtgccggag caatggacga
     2641 accaggccgc gagaaaacgc aagagtcgct tcgacaaagg aaacgaccga aatggaaggg
     2701 aaggtcaagg tgccatgaaa acctgcggat gaagcccctc ccaacgtcaa ggataggatt
     2761 ccttgtgtcg tcaatcggat gccgatgaga atgtcggata cttgtcgacg atgattaaaa
     2821 gcggaccctg attcccatgc ttgaagcacc atgacagctt cgcgcaggag acattccgca
     2881 atcaaaaacg ggtgaacgtt tccgaaatca cgtcgcacat caccgatacg aacgtggaaa
     2941 gggccagtga acagaaggga atgaagttca ttccgttcga cgttatcgag cacgggttcg
     3001 tcgtcgtaga cccgcagcga aaactctgtc gaaaaagata cggcagcatc gctttgcgct
     3061 tcgaccacca cgtcgacgat ttcgatcgag cgtgaagagg tctgccatcg caacgattcg
     3121 agaaaacgcc gtgctgcatc gaggtctctg aaaagtggtt ccgttttgtc tttttcttga
     3181 tctagcacgc tttcgatggc gttcatgatt ccgcggagca atgaaatccc taaaacgcgt
     3241 cgttcttgaa cgacgacatg agggtgttct ccagcttgga agacagtgac gagcacatcg
     3301 ttgtcgacac gttccagccc aagagcccac gcatctcgtt ggtcatagaa gcgcacggtg
     3361 gctcttgtgt gtcgatggat tgccaagtcc gccgcggcgt aagcaagatc tcgcaacagg
     3421 ggtatggcgt gatcttgccc gatcctggcg gtgacgttgg ttccatcgac gagcacatcg
     3481 aggacgccgc gcaggttcga aaacatccgt gaggcggaat gggtggaccg ccgatcgatt
     3541 acgatctgaa aagcacgcat gtgttgtgct catcgtagga tgctgttcgc aacgggcgaa
     3601 cttttcggat cgttccgtca aggactggac accgagacaa agaggcgcta gccgggggag
     3661 atgcgctcga tgcagcggtt ttgggcttcg ctgctcggat tgagccttgc ttgcggtgtg
     3721 gcgttcgcct tggttggctt caccgtggat gacgctttga taagcgcgcg ttacgcttcg
     3781 catcttgcgc aaggggtagg tcatcgattc aatgggtcgg ggcccttgac ggatggcgtg
     3841 acgcctttgg tatggcctta tcttctttgt tgcttcggat cggacccgct tcgattattc
     3901 gccgtggctc gatgggtggg gatgatcggg tggctcggaa cctcgtgttt tcttggaatg
     3961 catttggcgc gcgtggtggg cccccgatgg tcgctggggc atttgtcgtg ggtagtggcc
     4021 ttcggcacgc cggccgttgg ggcttgggca agcgcgggaa tggaaacgtc gcttgcctta
     4081 tgtcttgtga ccgtggctgc ggtgatgggt gatagagaca aaaggaaaac cgcgagtgtg
     4141 ctcgtcggcg tgtcgtgtct gttgcgccca gaattgctgc cattcgcgct tacgcttggg
     4201 cttggaagcg cctggttgga cgagcgtttt catcagcaaa gaacagttcg aagcgttcgc
     4261 tccttgttga ttcatgggtc gctggtgatg gttccttttc ttctcgtcat gttgattcga
     4321 tggaagatgt tcgacgcggt ggcgccgttg gccttgcgcg cgaaaccctc ggatattact
     4381 catggaattc gctatgccct cgccacctgg ttgattgctg gcccgccgct ttcatgcatg
     4441 gctccctggg catttcgaaa gttacgtggc tggcccgcct ggctgttggc tggctggcta
     4501 gttcatacgg tctgttgtgt tttggtgggg ggagattgga tgcctttgtc acggctattc
     4561 gttcctgtct tgcccgcgtt ggcgatcgtg ttcgcgcatc tcgcctcggt ggcccatgtc
     4621 gcctcgacga ttgcgcgtgc tctggtttgc atgatgggcc aagcgttcgt gtatgcgaca
     4681 gtgggagttg gtgcccatca cgtggtcagg gaccgaatgg tgttgatcga acaactgcgt
     4741 ggacagctac ggccggatga tgtggttgct tgtttggatg tcgggtgggt aggcgtggcc
     4801 catcgaggtt cggtatttga tttggcgggt gtaacggatc cgcaggttgc ggcgttgccg
     4861 ggggggcata cgagcaagcg tgtcacggcc gagatgttgg atgcgcgagg agtcacgcat
     4921 cttttggtgc ttcttgctcg aggagaatcg ggtttggatg cgtggcaatc gtgcggcttg
     4981 ggtcgttccg tggaagtacg gctttgtcga tcggaagccg tacgggcgaa gtttcatccc
     5041 acgcggctcg ttcgctctac ggataccttg cagtacctgc tgctcgagcg cggctcatca
     5101 tccgggcggg gacagcggta gtagctgggg gctccccgag cagatggata cgtcgaaagc
     5161 aatccgtggt gtccaggagc gtagcaggac cgagtcgggc aaagaaagga tgtgcaggcg
     5221 ctggtttccg aaagaagcgg cggtgcttcc catgagaaga tcgacgcgtt catcggggat
     5281 tgggatggcg aaatgggtag agggggaacg aatatcgaca tgatgcagga tcagcgaagc
     5341 gagggcttgg atgatggccg agcgggcagg ggagggatgg ctggggccga gtgcattggc
     5401 ggcgtcgtgt tgcaaggcaa ggagcaattc ttcctcggag taacgcgacg ggtcgtagcg
     5461 gtggatgccg gaggcggaca ggatgaggtc ccagcctggg ctgtagggtt tgatgtccac
     5521 gacgggtgtt ttgtcctgaa agtcgagtcg gtcgagcagc agggttgagc cttgacgttc
     5581 gagaagtcga gcgcaggtca ggcccagggg gttgggtcgg acgggcgatc gcatgctgaa
     5641 cacgccgcgg ggtggagatg agggggaaga ttttcgaggt cgggcttgga gtaggtcgcg
     5701 ggcggcgttg tgaaagaagc cgatgatcca gatatgagag caggagtcga ttccatcgag
     5761 ggcgttgcgg taggcaggga agatttcgat ggtggcgggg gcaccgtggc gagggacctc
     5821 gtgagaggtg tggaaagggc tgtgggcgat gccgatgggg ttgagagaaa tcggagccat
     5881 taaagaaaaa cgcctgtgat tacaggcgtt tgtggcgacc ccacggggac ttgaaccccg
     5941 gttaccggcg tgagaggccg atgtcctaac cactagacga tggggccagg ttcgaggata
     6001 tgttgaaccg cgaaggcggt agctggggga cagggattcg aaccccgata tgcagagcca
     6061 gaatctgctg tcctaccatt gaacgatccc ccagtgagat cattgaaata aggacaaagc
     6121 aaggatgcac caggcatccg taacgggatg ggaatcttag cggcgagatt tgtggagtca
     6181 agaaaaatat attttgcgga agaaataatt tcgcccttga cccgtgggca gggagcctta
     6241 tcttgcggcc gcttggcaga ttagcagcca ggacattcga gtgctaacaa gaagaaccca
     6301 gcggttggca acaccgacct agggttcccc atcggaggtt cacgctatga aattgaggcc
     6361 tttgggagac cgcgtcgtta tcaagcgtgt ggaacaagag gaaaagacca agggtggcat
     6421 catcatcccc gataccgcca aagaaaagcc agtggaagcc gaggttatcg ctgtcggtcc
     6481 cggaaaggcg ctcaaggatg ggaaaatccg ccccatcgag gtgaagcagg gcgaccgtgt
     6541 gctgatcggc aaatggtccg gaacggagat caagctcgag ggcatcgagc atgtcatcgt
     6601 gcgtgaagaa gatattcttg cggttgtaag ccgctgaggg tgaggagata cagcaatggc
     6661 agccaaggaa atcaagtaca accgcgcggc tcgtgccgcc atgcttcgtg gcgtcgacgg
     6721 tcttgccaat acggttaaag tgacgttggg ccctcgtgga cgcaatgtcg ttctcgaaaa
     6781 atcctggggt tctcccgtta tcaccaagga tggagtgacg gtcgccaagg agatcgagtt
     6841 cggtgacaag ttcgagaata tgggcgccca gatggttcgt gaagtcgcca gcaagacttc
     6901 cgacaaggca ggcgatggca ccaccaccgc cacggtcctc gcccaggcaa tcttcgtcga
     6961 aggcatcaag ctcgtcgagg ctggcgtcaa tcctatggac ctcaagcgag ggatcgatgc
     7021 ggccgtagaa aaggtagtcg atgccgtcaa gggcatgtcc catgccacca aagaccgcaa
     7081 tcagatcgca caagtcgcca ccatttccgc caatggcgac accaccatcg gcaccaccat
     7141 cgccgacgcc atggaaaagg ttggcaaaga aggcgtgatc acggtcgagg agaacaaggg
     7201 aaccgaaacc gaactcgatg tcgtcgaagg tatgcagttc gatcgcggct atgtatcgcc
     7261 ctacttcgta acggatgcgg agaaaatgga ggcggtgctg gaagaagctt atgtgctcgt
     7321 ccacgaaaag aagatctcca atatgaacga cctgcttccg ttgctggagg cggtggttcg
     7381 agaagggcga ccgttgctga ttctcgccga ggatatcgaa aacgaagcgc ttgcgacctt
     7441 ggtggtcaac aagctccgcg gcacggtcaa ggtctgtgcc gtcaaggctc ccggattcgg
     7501 cgaccgacgc aaggaaatgc tcaaggatat cgcgatcttg accggtgcca acccgttcat
     7561 ggaagacctc ggcctcaagc tcgagaaagc caccgttcgt gatctgggcc gtgccaagcg
     7621 aatcgttgtc gacaaggaca acaccaccat cgtggaaggc tacggcgaca agaaagaaat
     7681 caagggacgc accgaagcca ttcgcaagca gatcgaagat accacgagcg attatgaccg
     7741 tgaaaagctc caggagcgcc ttgccaagct cgcaggcggt gtggccgtcg tgcgtgtggg
     7801 tgcccatacg gaaacggaaa tgaaagagaa gaaggcccgt gtggaagacg cgctcaatgc
     7861 gacccgggcc gcggtcgaag agggaatcgt accgggcggc ggcgttgcgc tggttcgcgc
     7921 ccgaacggct ctcgatacct tgaatttcaa cgatgagcga cgccatggtg tggctatcat
     7981 tcggcgcgcc atcgaggaac ctctgcgcca gattgcgacc aatgcggggg tcgaaggagc
     8041 ggtggtgctg tcgaaggtag ccgagggcaa agatgcgttc ggtttcaacg ccgcgacaga
     8101 ggttttcgag aacttgctcg aagcaggcgt actggatccg gccaaggtcg ttcgatccgc
     8161 cctcgaaaac gcggcaagcg tagcgggact catgctcgtc accgaggccg ccattgctga
     8221 aaagccgaag aagcccgtca aggatgctgg cggcgccggt ggtatgggag gaatgggcgg
     8281 catgggtggt atgggcggat tcgatatgtg atccgttccc cgatgccctc atcttttccg
     8341 cagtccttcc atcctcgata aattacctat aaattccatc acataaccag gtttcatgaa
     8401 gtgacacctc agggttctca cgcacgcaaa cctgcgtttc gtggaagctc atcccatcaa
     8461 atatatcttc cttaaacact tgaaaactta atagtaacct attgaattat aaggatatta
     8521 ctaggttggc acacctttgt gttccccagc gcgacgagaa gtccagcaag accggaaggc
     8581 acgggacttt ggccgatggc tgccaaatgg gtatgctcta tccaatgagc gatacgctat
     8641 ttgaaaaatt cggacgggag ttcgaagagg ggttcgtttt gtttcaggaa gggcaacccg
     8701 gaaacgagat gttcgtcgtt caatccggcg taattcgcct caccaaacga gtgggacgcg
     8761 aggacaaacc tcttgcgttt gtgggagctg gcgagttttt gggagagatg gccatcttga
     8821 atgcgaaacc aagaaccgca acagccacgg tggcagaagg tcccgcccgc tgcctggtca
     8881 tcgatgcaca aacgttggaa gtgatggtga agaagaatgc cgagatcgcg cttcgcctca
     8941 taaaaaagtt ggcgaagcga ttggattccg cggatgcttt gatcgaaatc ctgatgcatc
     9001 gggaccccaa agcgcgggtg ttgctcgcgc tggttcgtca cgcggaggat tatggtgagg
     9061 cgaccgacca gggaatggtg cttcgctcga cgggccagga gttgggtgag caggtggggg
     9121 tcgatggtca ggtggtggag caggtgatgg cgcgattgcg tcggttgcga ctgatccagg
     9181 aaatggaaga cggacgccaa gtggtgccgg atttgagtcg tctcgaagag ttcatcgagt
     9241 ttttggacca gccgacaccc ggtggctgac gtcgtgaatc catgctccga agatcatttc
     9301 gtcgcatctg ccgaaaaaac cgcagagttt ctttcgctcg gccacgatgc ccgtgacaag
     9361 ccacagtcgt ttccggacca agagcgtttc caggcaaggg cagatgtcag cgcggaggca
     9421 attatgctcg gcgatctcgg gccggaaccg cgcggcttgg tgggaaggat gtcagcgcat
     9481 gctcggcgac gtcggacgcc aggtttcaac cagagcatag ccatagggta cgttttgatt
     9541 tatccaccac ggcagaggcg aggtgtggtc gcgagttgtc cggtgcgagg ccgcggcttg
     9601 ttaccgattc tggacgggta gggggaaaac catcgcgcgg gcaacgttgt ctgccgacgc
     9661 cagcggccat gttcatggtc gaggttcaag gatagccatc gatagggtcg agggcgatgc
     9721 cgaccaccgc ctggggcatt cgtcatcgtt cgggaccggg ggtgcatcat ccgctttcct
     9781 gcccctgcga tctcgttagt tgacataatg tatcttatgc gacttttgta tattcgaaat
     9841 tccccggctt ctacgcctct ctctcacgaa atcctatcaa ccccacccag gcccaacccc
     9901 aaaccaagct tcgctctgtg cgcatcttac ctccgccaat gcatctgcat cacgatccat
     9961 caacccctgc ccacaggtgc cagcgccttc gtcaacgcaa cctactccca ccatgccctc
    10021 ccgctatcga gcgtcttcag acctcgcacg atcaccgcgc catgatgcca cccacgaccg
    10081 tgctcccgat cgactccgta gcgacagcat gagcgtgagg aattgtctgg gcttccaccg
    10141 gatccgacgc aaaaaaccgt atttcatgcc gttttcgatg ccgtgccatc cataagctgc
    10201 acgcgctgtc attccgccat cccttgaatc gactcaccgt cgtcgaccct ggccccgcgt
    10261 aggcaacatc tcaattttca aagctttttt agtaagttac cgccacgcct cctcgcaacc
    10321 tcctgctcca cgacgtctcc gtcgtgaggt attgtgcctg ctcccgcctt gcccccatgg
    10381 cccgcgatca gccgtcacaa cggagcaccg attaaaattt gtttgtattt accaagggtt
    10441 agatcatccg cccgaccgta acggcggaga tacatgcagc gataaccacg ccccaccatc
    10501 ggatagcacg aaggctcgat ttccgtaggc tgcaggcgcc atcgagaagg tcatccccaa
    10561 atcaatgccg tcgatcacct tgccgttcag c
//
LOCUS       DAOSOR010000145        10570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6272_length_10570_cov_4.466191, whole genome shotgun sequence.
ACCESSION   DAOSOR010000145 DAOSOR010000000
VERSION     DAOSOR010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 10570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10570
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6272_length_10570_cov_4.466191"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="PK710_09890"
     CDS             complement(<1..876)
                     /locus_tag="PK710_09890"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOT10070.1"
                     /translation="MKAAQKLREWFDSHQSQVRDDIVRLTMEMVRQRTVNVTPDKLIE
                     HPYLAIRGEEWRVAAIVSRELEAWGIPFTRHERQQGRPNLLGRMGRNENGKRLLVAAH
                     MDVVPAGDPSEWKKIKNPFEPEVQQGMLYGRGVLDNKGPLASALVAVKMLREAIGSEA
                     LAGQLQIAALSDEEATGEDGVDYGVGYLLEEGLIDATCAIVPDIGEFMRSIDIAEKGG
                     GVMRVTARGVQAHGSTPERGVNAIHKMANLLQRFEAFPFVYEPHPLLGHPTINVGLIH
                     GGAAPNIVAGSCSFLV"
     gene            complement(915..2042)
                     /locus_tag="PK710_09895"
     CDS             complement(915..2042)
                     /locus_tag="PK710_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOT10071.1"
                     /translation="MAALVAWLVAAGMTLGSPASAHWQAQAPDHRDQRLLDVCGPGGR
                     VLHDVAYAMATRLLEGQTAWTTLELSEHLRARGEPHPAVRAWSLRMPRMNRNRAEKGL
                     KSWLASHPEGQRRRCGIASIEDSQRGDVLTMLTVAVLADLLTPIPKTVRQGQWITVDA
                     VLHDGAVEGRVVVLGPRGLPRVIPSSFDPATARVVARFMADRQGDWLLQVVGTTDAGP
                     KPMLEAQVRVGPRQAVTHVPVPGEAAGHGLSDEQAMVAKLNAARVEEGLLPLVVDAQL
                     TALAREHAKTMMASGAIGHDMGDGSPPQRLARAGGCAKEVGENVSRATSVTLAHLALW
                     KSVSHRTNLLHPRYERLGVSALRDEKGMLWIVQIFAGPCTS"
     gene            complement(2089..3396)
                     /locus_tag="PK710_09900"
     CDS             complement(2089..3396)
                     /locus_tag="PK710_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10072.1"
                     /translation="MTIASAVSRSAGTIVILSAMVACSPQKPPENAETPKANPTAPEW
                     LGPYDEAQKTVLAMPLDATIEQRAHRTYPDLTRFSFAGDSPISVPDDLLMFANHVSEV
                     LAASPLLYVFRTGPASLANIHALFGPKPVDDPDPWQRVAVDAQGSPSLVPSLPTDDVR
                     PLLDQGMKAGPKGLDSLKQAAEKSKTVPGVQAMLADAALAVGDLDTADQAARVALAAD
                     PSFPLSFRILAEVRWRHHQQDRAKEAIARALALYPTYPRAWKVAEVIVGHPIDRAVVV
                     EQPFIEVNDKGAIVVVSCDRPFCVGYASCKAAFRFEPVFRAAMLQEPESAPYHLSVTE
                     EVVCLQAGLGTHMAEREKNPAISDPTAELLIQLAAEKGLSSFALFEILGFHRPEWLRI
                     APRPVHEALVHHILVHVLGTPMHEQPSHDDIITARRTNTGEGR"
     gene            3455..4924
                     /locus_tag="PK710_09905"
     CDS             3455..4924
                     /locus_tag="PK710_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOT10073.1"
                     /translation="MAEWTRGAKVGVFALACVGAGYGLWTFVNPSAGTSGGYTVHAYL
                     NDASGLASYSRVMVAGIPIGSIESIRLDKGKARVDIKVKPDVALYENADLAKRSSSLL
                     GEFVIVIGPGDDSQRRLKDGDEIKRVQEPITTDQIMNDLGHVANRVKSVSDSLANSVG
                     SEQGEEDIRATLKNLAEVTDQLNQTVRENRQSLNQIIHNVERISDRSEPQIAQILENV
                     RVITGEVRTLVAEEQGDVKGSMGTLRDTLNRVNKASVDLQGSLHHINSVAERIDRGEG
                     TVGRLTKDETLINEVEEVVEGVGDFVGGMSRLQTVVGLRNDYNFQANTVKSYIELRLQ
                     PREDKYYLLELINDPRGLTRVEQISVDTDNPNDPNHYREIRTVTQNTLRFSFQFARRL
                     GPLTGRFGLKESTGGIGLDLHLLQDRFELQQDLFGFSEQLTPRWRIGLSYEFIRRLWL
                     ITGVDDVVDRHRRDYFIGLQLRFLDEDLKSVLPFAPTSF"
     gene            complement(4949..6514)
                     /locus_tag="PK710_09910"
     CDS             complement(4949..6514)
                     /locus_tag="PK710_09910"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOT10074.1"
                     /translation="MRWRIVGIALVLHLIGCEQEATSRPQWLVAVSTDAVVPLMGNRL
                     LVELLNEQGQLACPSCRREAVVALPENWPATFGIAPPTGSQEVFVRVQFYRADRPSLS
                     IESLGRLPPLHDGVNSVSMHLSARCLGKPVHDTQEGFETCSELDPSIMMPPPVLEPYR
                     DRGPCGGVGTTDLVPSAEHRYCDPTWRTKETKGMVCVPGGMFFLGDELAPVPSKDVRA
                     ITYPERLVRIRSFFMDRREVTVREYREYLINTNQYQEQNCPEGLHCRDESEKKSSKAI
                     HCTFPLDNASHYDGMPLNCVSWRQAYEYCRAQGKRLPTEAEWEYVASQGEQEKRYPFG
                     DSLIGCDGVYVARGESSWRDDDSCLQGKEPGPAGDKFPHDRVVLPWTSWSGDSRCGGP
                     IDYPDTVKLDDDDGNAIQALAGNVSEWTQDRFAPYEKMADDDLPCWSDLSDPWIDFTD
                     PGTKACKVTDPDDSVDGVKNTTKMAVRGGSWRDPMEATWSATRNSAPARLTTPRPEIG
                     FRCVLDPWEHEWE"
     gene            complement(6511..8121)
                     /locus_tag="PK710_09915"
     CDS             complement(6511..8121)
                     /locus_tag="PK710_09915"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT10075.1"
                     /translation="MVRSRRPEVALTLILLAGTAQCGSSTDAPGGQALLYIDTDAPSI
                     SLAREQEHAFLTEAAVDLLRIDILDPDNRVVSSTDVAAPDEMNWPVSFGIKGLEGAVV
                     SRVRLRAFRSQRAAVTTDPNTNTKVFEPIAGYVIDRVVEVPVPYEKGVFAFRVVLRSA
                     CRGYRPDFTRHATCVAEDRKEAGFRDDIESVAADTRPAREALWWPGDLQRTDSSQSQD
                     CANAKDHCPPHSVCIPGGFFMLGNPRVVGFGPNTDAVPPHPVVMKPFCIDATEYTVER
                     YKGSEMPINTMGRSETDPYGNELCTWVPDNSDVNLPMNCVTHEEAAALCEQSGGRLPT
                     EAEWEYVATNLGRGSLFPWGDAPPTCNSAVLEQQETDFLFAPECGFDGLLPVAKGENG
                     KQDIVKVWQDPTDNGGSDQGVWHMAGSLSEWTLDSYTSYGNVEPTTNCWQRQGLFVHP
                     CCGIRGLTKTIRGGSFAEPLEYAYAPLRRGDQKTTSSALVGFRCVYSVQGEEEDPVQY
                     IEPNPEQPFDPSYDAGPIGDEEPPGGIQ"
     gene            8210..9205
                     /locus_tag="PK710_09920"
     CDS             8210..9205
                     /locus_tag="PK710_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10076.1"
                     /translation="MGHLPRLIRHTLALLIAVVGCSTSMRMNDTGDEMEALPTDNRLA
                     SRCSKCFSEDCAWQTTECSSDPGCARLLTCANEGPLDGQGLLDIDWLRNHCRPVEGVS
                     ATLWNSLVSCVTGSMCCEEVPENGGNPGSGGGGGGGIVPDAQSGAVDTGADQEYDSTC
                     SQCLKELGTATDASSTDTQCRAALDDCKQGECHEYLTLYQECLTANTNSERGLEACMF
                     QGLEQGKKLASNASSAAFAMFMQRVFGCLTGSCASLCVPADVRPCIACQQQSCGDDLA
                     LVRSSNDAILGIWCRSYCQWNQDEQCPGICTGFMTEAADILQSYGQCVKASCPEC"
     gene            9253..10245
                     /locus_tag="PK710_09925"
     CDS             9253..10245
                     /locus_tag="PK710_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10077.1"
                     /translation="MKIAPIWLAWVFVLGCQAATALPESDPMLQNGGSRESATSCSRC
                     YEEACAWETIECAADPGCAQWLSCAQGCNQEESCLSSCPVPGSSLGDSLRDRLNRCIT
                     RTSCCAVAAPGGLDQTKGAGGDGGQGGGGGGGHGGNNGTLDAAPTTPEDGASCDENNC
                     NSCLISIKTANVDEGSECSDKECREIADNCYHDEGKPSPCWNYMTCAEQNASCAAVPV
                     TNADLVIQTLSCAEKYCPVSCLSSCKKCQFNSCRDELKAFLSSTAAQHLVFCRDECKS
                     DPTPQACIRKCVEQKEQMEQMEQMEQMEQYEEALSVVGELNQCTMEWCKDKCTP"
     gene            complement(10262..>10570)
                     /locus_tag="PK710_09930"
     CDS             complement(10262..>10570)
                     /locus_tag="PK710_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10078.1"
                     /translation="LGFEVGLETNGTLPCPPEIDWICVSPKAGAPLVQTAGDELKLVF
                     PQEGLDPKDFVSLAFTTFSLQPRHDANLLENIRICLDYCASHPRWRLSVQLHKFLGIP
                     "
BASE COUNT         2247 a         2969 c         3058 g         2296 t
ORIGIN      
        1 gacgaggaag gagcaggatc cggcaacgat attgggagca gccccaccgt ggatgagtcc
       61 cacgttgatc gtgggatgtc caagcagcgg atgcggttcg tagacgaacg ggaatgcttc
      121 gaaacgctga agaagattcg ccattttatg tattgcgttc acgccccgtt ctggtgtgga
      181 accgtgagct tgcacacctc gggccgtcac acgcatcacc cctccgccct tttcggcaat
      241 gtcgatgctg cgcatgaatt caccgatatc cggtacgatg gcgcaggtgg cgtcgatcaa
      301 cccttcttcc agcaagtacc ccacgccata atcgactcca tcttcccccg tggcttcttc
      361 atcgctaagg gccgcgattt gaagctggcc ggccagcgcc tccgagccga tggcttcgcg
      421 tagcatcttc acggccacca atgcgcttgc gagcgggccc ttgttgtcga gcacccctcg
      481 tccgtacagc atcccttgtt gaacctccgg ctcgaacggg ttcttaatct ttttccattc
      541 cgacggatcg cccgcgggaa ccacatccat gtgggctgcc accaggaggc gctttccatt
      601 ttcgttgcgc cccatacgac caaggagatt cggacgtcct tgctgtcgct cgtgacgcgt
      661 gaacggaatt ccccaggctt ccaactcgcg gcttacgatc gcggccacac gccattcttc
      721 tccacgaatc gcgagatagg ggtgctcgat gagcttgtcg ggcgtcacgt tcaccgttcg
      781 ctgtcgaacc atttccatgg tcaaccgaac gatgtcatcg cgaacttgtg attgatggga
      841 gtcgaaccat tcccgtagct tctgggcggc cttcatggct ctggcttaca ccacaaccaa
      901 ggaaattcga agacctagga cgtgcatggt cccgcgaata tctgcacgat ccacaacatc
      961 cccttttcgt ctcgcaacgc ggatacaccc aaccgctcat agcgcggatg caagagattg
     1021 gtcctatgcg atacgctttt ccacaacgca agatgcgcaa gcgtcacgct cgtggcgcgt
     1081 gatacgtttt ctcccacctc cttggcacac cctccagcgc gagcgagccg ttgtggcgga
     1141 ctaccgtctc ccatatcgtg tccgatagcc ccgcttgcca tcatggtttt cgcatgttct
     1201 cgcgcaaggg ccgtgagttg cgcatccacg accagcggca acaacccttc ctccacacgt
     1261 gcggcgttga gcttggcgac catggcctgc tcatcggaaa gaccatgacc cgcggcttca
     1321 ccgggcacgg ggacgtgcgt cactgcctgt cgaggcccca cccgcacctg agcttcgagc
     1381 atcggtttgg ggcccgcgtc tgtcgttccc acgacttgca acagccaatc tccttggcga
     1441 tccgccatga accgagccac cactcgcgcg gtcgccggat cgaaactcga aggaatcaca
     1501 cgcggcaagc cccgtggacc aagcaccacg acccgaccct ctaccgctcc atcgtgcaat
     1561 accgcatcca cagttatcca ttgtccctgt cgcaccgttt ttggaatcgg tgtcagcaag
     1621 tccgccaaca cggccacggt gagcatcgtc aacacatctc cgcgttgtga gtcttcgatg
     1681 ctcgcgatgc cacagcggcg acgttgcccc tccgggtggg aagcgagcca cgatttcagt
     1741 cctttttccg cgcggttacg gttcatgcga ggcatgcgta aagaccatgc acgaaccgcg
     1801 ggatgaggct cgcctcgcgc gcgaagatgc tcactcaact cgagagtcgt ccatgcagtc
     1861 tgtccttcga gcaatcgtgt tgccatggcg taagcgacgt catgcaacac tcttcctcca
     1921 ggcccgcaga cgtcgagcaa cctctgatcc cgatggtctg gcgcttgcgc ttgccaatgc
     1981 gcgctggcag gtgatcccaa cgtcattccc gctgccacga gccatgccac gagcgcggcc
     2041 atacgcccac gccatcgaag gagtgtgtga tggcttgcat cccccacctt atcgaccctc
     2101 gcccgtgttc gtccttcgtg cggtaatgat gtcgtcgtgg gaaggttgct cgtgcatggg
     2161 tgtaccaaga acgtgaacaa ggatgtgatg cacgagagct tcgtggacgg gtcgaggggc
     2221 gatgcgaagc cattccgggc gatgaaaccc taggatctca aaaagggcga aagaagacag
     2281 tcctttttcc gcggcgagtt ggatgagaag ctccgcggtt gggtcagaga tggctggatt
     2341 tttttcgcgt tcggccatgt gggtgccaag tcccgcttga aggcatacga cttcttcggt
     2401 gacggataag tgataaggcg cggactccgg ctcttgcagc atcgccgcgc gaaagacggg
     2461 ttcgaagcga aaagctgcct tacaggaggc gtatccaacg cagaaggggc gatcacagct
     2521 cacgacgacg atcgcgcctt tgtcattcac ctcgatgaag ggttgttcga cgaccaccgc
     2581 tcgatcgatg ggatgtccaa cgatgacctc cgcgactttc caagcacgag gataggtcgg
     2641 gtacaacgcc aacgctctgg cgatggcttc cttggcgcga tcctgctgat ggtgtcgcca
     2701 ccgcacttcc gcaagtatgc gaaatgaaag aggaaatgaa ggatccgccg cgagggcgac
     2761 acgggcagct tggtccgccg tatccaagtc tccgacggcg agggcggcgt ccgccagcat
     2821 ggcttggact cctggtaccg ttttggattt ttctgccgct tgcttgaggg aatccaagcc
     2881 tttgggtcct gctttcatgc cttggtccaa cagaggccgc acgtcgtcgg tgggcaacga
     2941 ggggaccaac gagggcgagc cctgggcatc gaccgcgaca cgttgccagg ggtcggggtc
     3001 atcgacaggc tttgggccga acaatgcgtg aatgtttgca agggaggcgg gacccgtgcg
     3061 aaaaacgtac aatagcggcg aggctgcgag gacctcggaa acgtgatttg cgaacatcaa
     3121 aaggtcgtct ggaacggaga tagggctgtc tcccgcgaac gaaaaacggg tgagatcagg
     3181 ataggtgcgg tgggcgcgct gctcgatcgt agcgtccaaa ggcatggcca acacggtttt
     3241 ttgggcttcg tcataagggc caagccattc gggcgcggtg gggttggctt tcggggtttc
     3301 cgcattttca ggcggttttt gggggctaca ggccaccatg gcggaaagaa tcacaatggt
     3361 accagcgctt cggcttacgg cgctcgcgat cgtcatgcac ccgatcgtag cttactgggt
     3421 cagggtttgt tagtttacgg tcataggatg acccatggct gaatggacac gtggggcaaa
     3481 agttggcgta ttcgccctgg cgtgtgtggg ggctggctat ggtctatgga cgttcgtgaa
     3541 cccctcggcg ggtacttcgg ggggatatac ggttcacgcg tatctcaacg acgcctccgg
     3601 gttggcttct tattcgcgtg tgatggtggc cggtattccc atcggcagca tcgaatccat
     3661 tcgcttggac aagggcaaag ccagggtaga catcaaggtc aagcctgatg tggcgcttta
     3721 tgaaaacgcg gatctggcca agcgctcttc gagtctgctc ggagagttcg tcatcgtcat
     3781 cggaccgggg gatgactcac aacgtcgctt gaaagatggc gatgagatca aacgtgtgca
     3841 ggagccgatc accaccgatc agatcatgaa tgacttgggc catgtcgcca atcgtgtgaa
     3901 gtcggtatcc gatagcctgg cgaactcggt aggttccgag caaggggaag aagacatacg
     3961 agccacgttg aagaatctgg cggaggttac cgatcaactc aaccaaaccg tgagagagaa
     4021 tcgtcaatcg ctcaatcaaa tcatccacaa cgtggagcgg atttccgatc gaagcgaacc
     4081 tcagatcgcc caaattctcg aaaacgtccg tgtcatcacc ggcgaagtgc gcacgctggt
     4141 cgccgaggaa caaggcgatg tcaaaggttc gatgggaact ttgcgagata cgttgaaccg
     4201 cgtcaacaaa gcgagcgtcg acctgcaagg ttccttgcat cacatcaaca gcgtggcaga
     4261 acgaatcgat cgcggagaag gcacggtggg gcgcctgacc aaagacgaaa cgctgatcaa
     4321 cgaagtggaa gaagtggtcg agggcgtcgg agatttcgtg ggaggaatgt ctcgtctgca
     4381 aacggtggtg gggctgcgca atgactacaa cttccaagcc aatacggtga aaagttacat
     4441 cgagttgcgc ctgcaacccc gcgaagacaa gtactacttg ctcgaactca tcaatgatcc
     4501 gcggggcctt acccgcgtcg aacaaatcag tgtcgatacg gacaatccca atgatccgaa
     4561 ccattaccgt gagattcgga cggttacgca aaacacgctt cggttttcgt tccagttcgc
     4621 gcgacggttg gggccgctga cgggtcggtt tggcttgaag gagtccacgg gcgggattgg
     4681 tctcgacctg catctgcttc aagatcgatt cgaattgcag caggacctat ttggattcag
     4741 cgagcaactc acaccccggt ggcgtattgg cttgagttat gaattcatcc gacgactttg
     4801 gctcatcacg ggtgtggatg atgtcgtcga tcgccatcga cgtgattact tcattggctt
     4861 gcagcttcgt tttttggacg aggacttgaa gtccgtgctg ccatttgcac ccacgagttt
     4921 ttgaagtctc gtgccggtcg agtccttgct actcccactc atgctcccac ggatcgagca
     4981 cgcatcggaa gccgatctcc ggccgtgggg tcgtaagacg tgccggggct gaattgcgtg
     5041 tggcgctcca tgtcgcttcc atgggatcgc gccaggatcc gccgcgaacc gccattttgg
     5101 ttgtattttt gactccatcc acggaatcgt cgggatcagt gaccttgcaa gccttcgtac
     5161 ccggatcggt gaagtcgatc cacgggtcgg ataaatccga ccaacaaggg aggtcgtcgt
     5221 cagccatttt ttcgtagggc gcgaatcgat cctgggtcca ttccgacaca tttcccgcga
     5281 gtgcttggat ggcgttgccg tcgtcgtcgt ccaatttaac cgtatccgga tagtcgatgg
     5341 ggcctccgca acgcgaatcg cccgaccacg aggtccaagg aagcacgact cggtcatgtg
     5401 ggaatttatc accagcaggt cccggctcct ttccctggag gcaactgtca tcatcgcgcc
     5461 agctcgattc ccctcgtgcc acgtacacgc catcgcagcc tatcaaacta tccccaaatg
     5521 gatagcgttt ttcctgctct ccctggctcg ccacatactc ccattccgct tccgtgggca
     5581 agcgcttgcc ttgcgcacgg cagtattcgt aggcttgacg ccacgacacg caattgagag
     5641 gcataccgtc gtaatgtgag gcattgtcca atgggaacgt gcagtgaatc gccttcgatg
     5701 atttcttctc gctttcatct cggcagtgca gcccttccgg acagttctgt tcctggtatt
     5761 ggttggtgtt gattaaatac tcccgatact cgcgtacagt gacttctcgc ctgtccatga
     5821 aaaacgatcg gatcctgacg agccgttcgg gatacgtgat cgcacgaacg tccttcgatg
     5881 gaacgggggc gagttcatca cccaaaaaga acatacctcc gggcacacac accatgcctt
     5941 tggtttcctt ggttctccac gttggatcac aataacgatg ctccgccgaa ggaacgagat
     6001 cggtggtgcc cacaccgcca cagggccctc tatcccgata aggctccaac accgggggag
     6061 gcatcatgat cgatggatcc aattcagaac acgtctcgaa tccctcttgt gtgtcatgaa
     6121 ccggtttgcc aaggcaacgc gccgacaagt gcatactcac ggagttcaca ccatcgtgca
     6181 gcggtggcag ccggccgagc gactcgatgg aaagagaagg ccgatcggcg cgatagaatt
     6241 gaacgcgtac aaaaacttcc tgacttccgg tcggtggagc aatgccgaac gtggcgggcc
     6301 agttctctgg cagggcgacc acggcttctc gacggcatga tgggcacgcg agttgcccct
     6361 gttcattgag caattccacg agcaatcggt tacccatcag tggcaccacg gcatcggtgg
     6421 aaaccgcgac caaccattgc ggcctcgagg ttgcttcctg ctcacaaccg ataaggtgca
     6481 ggaccagggc aattccaacg atacgccacc tcattggatg cctccgggcg gttcctcgtc
     6541 accgatgggg ccggcgtcat agcttgggtc gaacggttgt tcggggttcg gttcgatgta
     6601 ctgcaccgga tcctcctctt ccccctgcac gctgtacacg caacgaaagc caacgagtgc
     6661 gctcgatgtg gttttctggt ctccgcgacg cagcggggcg taggcgtatt cgagaggttc
     6721 cgcgaaactg cctcctcgaa tggttttggt caggccccgg atcccacagc aaggatgaac
     6781 aaacaagcct tgtcgctgcc aacaattcgt ggtcggttcc acgtttccat acgaggtgta
     6841 ggagtcaagg gtccactccg aaagggatcc ggccatgtgc cacacgcctt ggtccgatcc
     6901 gccattgtcc gtcggatcct gccatacctt gacgatatct tgtttcccgt tttcgccttt
     6961 tgcgaccggt agcaagccgt cgaagccgca ttccggcgcg aacaaaaagt cggtttcctg
     7021 ctgttcgagc acggcggagt tgcacgttgg tggagcgtct ccccacggaa ataacgaacc
     7081 ccttccaaga ttggtagcca cgtattccca ttcggcctcc gtcggaagac gaccgcccga
     7141 ttgttcgcat agcgcggcag cctcttcgtg agtgacgcag ttcatgggca ggttcacatc
     7201 gctattgtcg ggtacccaag tacaaagctc gtttccgtag ggatcggtct cgctccgacc
     7261 catagtgtta atgggcatct ctgacccctt gtaacgctcc accgtgtatt ccgtggcgtc
     7321 gatacagaaa ggcttcatga caacagggtg gggaggaacg gcgtcggtgt ttgggccaaa
     7381 tccgacgacg cgaggattgc ccagcatgaa aaacccaccg ggaatgcata cgctgtgggg
     7441 ggggcagtgg tctttggcgt ttgcacagtc ctgggattga gagctatccg ttcgttgcag
     7501 gtctccaggc caccacaatg cttctcgcgc aggtcgggta tcggcggcca cggactcgat
     7561 gtcatcgcga aatcctgctt cttttcgatc ctcggcgacg cacgtggcgt ggcgagtgaa
     7621 atctgggcga tagccacgac aagcggaacg gagcacgaca cggaaagcga atacgccttt
     7681 ttcgtaggga acggggactt cgacgacccg gtcgatgacg tagccggcga ttggttcgaa
     7741 taccttggta ttggtgttgg gatccgtggt gacggccgca cgttgtgaac ggaaagctcg
     7801 aagccgcacc cgtgagacaa ccgcgccctc gagccctttg attccaaaag aaaccggcca
     7861 attcatctca tccggggccg cgacatcggt cgacgataca accctgttgt cgggatcgag
     7921 aatgtcgatg cgtaaaaggt ccacggcggc ttccgtgaga aaagcatgtt cctgttcccg
     7981 agcgagagat atcgaagggg catctgtgtc aatatatagc agtgcttgtc cccctggcgc
     8041 atccgtggat gagccacatt gagccgtccc tgccaaaagg atcagagtca gagccacctc
     8101 gggtcgtcgc gatcgcacca taccaacgat cttagcaggg gtttcaaaac aaaggagcat
     8161 gctcgagatg ccttgtttcc cttgcccgga tcttgcatga ttaaggatca tgggccacct
     8221 gcctagactc atccgtcata ccctggcgct gctgatcgct gtcgtcggtt gctcaacatc
     8281 gatgcgaatg aacgacaccg gggatgaaat ggaggcgttg cctacggaca atcgcctcgc
     8341 ttccaggtgt tccaagtgtt tttccgagga ttgtgcgtgg caaaccaccg aatgctcttc
     8401 ggatccgggg tgtgcgagac tgctgacttg tgcgaatgaa ggcccgttgg atgggcaagg
     8461 attgcttgat atcgattggc tacgcaatca ttgcagacct gtcgaaggtg tctccgctac
     8521 cttgtggaac tcgctggttt cctgcgtcac gggctcgatg tgttgcgaag aagtccctga
     8581 aaacggtgga aatccgggat ccgggggcgg tggtggtggc ggaatcgtgc cggacgcgca
     8641 aagcggtgcc gtagataccg gagcggacca agaatatgat tctacctgtt ctcaatgcct
     8701 caaggaactc ggaacggcca ctgacgcttc gtccaccgac actcaatgcc gtgcggcctt
     8761 ggacgactgc aaacaaggcg agtgccacga gtacttgacc ctgtatcaag agtgcttgac
     8821 ggccaatacc aatagcgaaa gagggctgga agcttgcatg tttcaaggtc tggagcaggg
     8881 gaagaaactc gcttccaatg cctcttcagc cgccttcgcc atgttcatgc aacgcgtttt
     8941 cggctgtttg acaggctctt gcgccagtct ctgtgtccct gccgacgtcc gaccatgcat
     9001 cgcttgtcaa cagcaatcct gtggcgatga ccttgctttg gttcgatctt ccaacgatgc
     9061 catccttggt atttggtgtc gttcgtactg ccaatggaat caagatgaac aatgcccagg
     9121 catttgtacc ggcttcatga cagaagcggc cgacatcctg caatcttatg gacaatgcgt
     9181 gaaagcgtca tgccccgaat gttgatccgt gatgggtttc gtggacaatc cccaaccgcg
     9241 aggagatctc cgatgaaaat cgcccctatt tggctcgcct gggtcttcgt tctcggatgc
     9301 caagcggcga cggctttgcc cgagtccgat ccgatgctcc aaaacggtgg tagccgcgaa
     9361 tcggcaacct cgtgttcgcg ttgttacgag gaggcatgcg cttgggagac gatcgaatgc
     9421 gcggccgacc cagggtgtgc ccaatggctt tcgtgcgccc aaggatgcaa ccaagaagaa
     9481 agctgtctat cctcctgtcc tgtgcctggc tcttcgttgg gcgactccct gcgggatcgg
     9541 ctcaaccgtt gtatcacgcg aacaagctgt tgcgccgttg cggcgcctgg tgggcttgat
     9601 caaacaaagg gtgctggtgg cgatggtgga cagggtggtg gtgggggcgg tggacacggt
     9661 gggaacaacg ggacgctcga cgctgcgcca acaacacctg aagatggcgc tagctgcgat
     9721 gagaacaact gcaatagttg tcttatatcc atcaaaacgg caaatgtaga tgagggttcg
     9781 gaatgttctg ataaagagtg tcgggaaata gcggacaatt gttatcacga cgaaggcaaa
     9841 ccaagtccct gctggaacta catgacctgt gcggaacaga acgcttcgtg cgcagcggtt
     9901 ccggtgacta atgccgattt ggttatccaa accctttcct gtgcggaaaa gtattgccct
     9961 gtaagctgtc tgagttcctg taagaaatgc caattcaact cgtgtcggga tgaattgaag
    10021 gcgtttctgt ccagtaccgc cgcgcaacac ctcgtatttt gtcgagacga atgcaaatcg
    10081 gatccaacgc cccaggcgtg cattcgaaaa tgtgtggaac agaaggaaca gatggaacag
    10141 atggagcaga tggaacagat ggaacagtac gaagaggcgc tgtctgtcgt aggggaattg
    10201 aaccaatgca ccatggaatg gtgcaaagac aaatgtacgc cctgaccgca ccgtcgtgat
    10261 ttcacgggat tccaaggaat ttatgaagct gcacgctgag ccgccatcgg gggtgagagg
    10321 cgcaataatc taggcaaata cgtatatttt ccaataggtt agcgtcgtgc cggggttgta
    10381 gggaaaaggt ggtgaacgcc agggaaacga agtccttggg gtccaagccc tcctgaggaa
    10441 acaccagttt gagttcgtct cccgccgtct gcacgagggg tgcgccagct ttggggctca
    10501 cgcagatcca gtcgatttct ggtggacagg gtaacgttcc gttggtttcg aggcccactt
    10561 cgaaacccag
//
LOCUS       DAOSOR010000146        10566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6277_length_10566_cov_3.997812, whole genome shotgun sequence.
ACCESSION   DAOSOR010000146 DAOSOR010000000
VERSION     DAOSOR010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 10566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10566
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6277_length_10566_cov_3.997812"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..1380)
                     /locus_tag="PK710_09935"
     CDS             complement(193..1380)
                     /locus_tag="PK710_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10079.1"
                     /translation="MSSHTLFFVTALAAIAVATACSDDDSNSYSSEGPPPATGGFGAG
                     GPDAGVGGSGAWAGSGGHEAGSGGGAAGVAGAAGQGTGATAGMGGTGGGVSGGGGAGL
                     GNPASIGYGARKTIVIDGANTGNEWGDDTLLLRDPAGDDARFLGTNWCAHEPPWDYAA
                     LHAAWDDQFLYIGIQYVNITDVVDPANLGGSESSQIHQMDLVQFVAFDTAAGSGYSVG
                     GDMWGKDHAFVGPDKPEYQFYFHSNFSQEGTYWGAWDGSALVPLTDGVKEPRLKGMAG
                     EFFVGNTLPGVDPQADDTNPGAYGMPSINYLTAGHDTKYDTFFELQIPLSLLNISADR
                     LDQGTIGVFAANGDGSAVDSIPNDPATSSTPGVSQSNSPLEWDATVDDDLYTSPFAWI
                     GTP"
     gene            complement(1456..2370)
                     /locus_tag="PK710_09940"
     CDS             complement(1456..2370)
                     /locus_tag="PK710_09940"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOT10080.1"
                     /translation="MLLHTFSVSGVETSLWIPPLVAFVVSFFTSMAGVSGAFVLLPFQ
                     MSVLGFTSPSVSPTNLVFNVVAIPSGVWRFVRERRMVWPLTWVVVAGTLPGVIAGGFV
                     RLHWLPDPTPFKAFVGGVLLFIGARLAKDVTRFSGASPKARSTAEPFVVQEQTWTWRK
                     IVIRFQGKEHACSTPGVFSLSVVVGMVGGVYGIGGGALLAPFFVTLYQLPVHTVAGAT
                     LMGTFVTSVVGVVFYQLIAPCYGQMSVAPDWLLGGLFGVGGVFGMYFGARAQRFIPER
                     WIKGGLAVLLAALAFRYLHDWAAKLMGG"
     gene            complement(2413..5436)
                     /locus_tag="PK710_09945"
     CDS             complement(2413..5436)
                     /locus_tag="PK710_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOT10081.1"
                     /translation="MTPRPLRISLPFQANSVELHGELQDRHHDPIPLHHRPDGNEWEF
                     AIDLAPGVYAYKLVVDGSWRLDPNNPRTRQRTVHRDNVWVVAGTPEPLLFAPALPHCV
                     PHPRGGYRITALLRKGFGSSLRIAWSEGRPDTESVMPMSRVSEEQEHIVFRSHLPTST
                     PFARFRFLLDDGTFVGQENGHDFDIPKCIDRLPSFWEQAVVYTIFVDRFRPQTDHSAW
                     ADDPGPDLPAYGHIDGITRSLDSLADLGVTVLHLTPIHVGASCHRYDVIDPLTVDPAI
                     GGEQALRRLLDAAHQRGLRVLLDFSMLHVGTGFAPYEQVKREGPGSSLAPWFRWRDRS
                     GHLQLDHYGGRVDAPLLNLDHEPVQELALQAVKHWTRFGIDGFRLDAAAQVPLSLGTR
                     IRDTLRENAPHSLVLGEVVPAHAWRWQQAGVVDAATDFSFHATMTSFLAERSIDAATA
                     AERIEYAVSEALSANRAVRFLSTHDHNRFATLCAVRGAPNRTALGMLMLVAMPGTPLL
                     VYGEELGMAAKIAALRPEGAWEDRVPMPWSWSETQIAFRSLTRSLLQARRNHPALHSG
                     DMQIVYAEDRLLVLRRALGNDIVDVVINASDSSVELSLDDDWLELLEPVASTGDVRVQ
                     DQQVCLGANSGLLAQRKPRQRPAGVIELMEKERKRRHNEAFASGRSEAPVRPSRIDFS
                     ITERCNVRCRHCINHSPDRVQQQTARTLSPAVLDRIREDLAHASYFGFVHGGESLTAP
                     IFFDVLEAIRFARAGAPTVIHLLSNGMLLTPDVVERLVQAGVSSISLSIDGASPSVHD
                     RIRVGSRLSVIEKHVEKMVELRHRHQLDLRIGLSCVVMTENQFELLQIVHLAARLGMD
                     WVKFEELVASTEFARQWLVDPQGEETRNLVEEAVKQGRRLGLTMVDHTAPPRVWRCKL
                     KDSPQMASFLGADQFANRSVIHPCRAAWEHVCIEPNGDVHIASFHGAVIGNVMAEPLT
                     MLWNGSVAQSERMRSRLERICGDGPVTCLSENEII"
     gene            5619..7442
                     /locus_tag="PK710_09950"
     CDS             5619..7442
                     /locus_tag="PK710_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10082.1"
                     /translation="MLKVKVKKMGQVVVGATSMLALGPLTMGCALDASGQETEQQETL
                     GQQSQAVEPGSATLFTSVQAYNQQEISVVVTNATTDDFIRVGEKLKMSCRAVDYWWLA
                     HPNGYEGYDDVKRLKKLNIQVTAHFYGNGVKKSSKTVAVNSWSNTPHAWELVGTTQEF
                     TVPANTDVIRFSYAVTDGGDASVNVQVGEDNTDRVVVFGARPEKHLVFENDFSTLRHK
                     IIEDGALVRGRDVLVGYTDYRANTLADTTSINTVIGQQKNYGRFGPDIIPLHGTLEHE
                     VTIGVQIDGVWGQEAPMVQRFDSAMVPRTQMYNRRFAFERRISLPANAKQVAVYFHIK
                     TYLRADYSQFPFVVEKYFADGERILVREVWDNNGGSGNDYHFGTEASTLPSVQPLPAD
                     IRRTVVFVQGETSPGQDMFVRGGVDHGVAEALLGKSCTNSQGDPTYGCSIPITNLNRK
                     NAYTDPWKVGDGLLDWYGVEAGQQGSVNGQQANGTAADWTTNYWPPEWGPEKTVAHDG
                     YGLEVLNRVCGMHCWMVDVDMDCAKAYHSPDGTPWFEIKTFISNGPGWEPDVEQGLMN
                     AYGTPEAPYVSKNHLARCGMVNVFRRGQSTAEFYRFDQFLP"
     gene            7600..8631
                     /locus_tag="PK710_09955"
     CDS             7600..8631
                     /locus_tag="PK710_09955"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOT10083.1"
                     /translation="MQSDLGTTLLTTSLMVVCYLIIVRLVDMNEKEPLWAMAAFFVLG
                     AGASVAVVQAVGTPILDLEPLWGAVIKEGARFGVLGVGVGGLLLYGRKRGWDEFNGLL
                     DGVVYGATVGLGFATAREVMNQLLVGAFAIPGEEAGMLAGFHKVFLFGLADGVFGAIV
                     GAGIGAAVEARAMVLRVVWPVLGVAAAILANWGYVVFGKGNGLGGNEGLIRAQIALVL
                     PVIVVVVVVVYALFSERKAIRVQLSSEQADGIVTAADLALLQNVVARETTYWKTLLSG
                     KIRFWRLMRALHNRQVQLAFVKQNASRETNEERKKRLEVEISNLRLNIREQQTALGKG
                     TTGDEGRES"
     gene            8628..9206
                     /locus_tag="PK710_09960"
     CDS             8628..9206
                     /locus_tag="PK710_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10084.1"
                     /translation="MSFIMRYWRIVVSKIPAFLLMVVAFQVSGCCCRGCCNALPAGGS
                     GSSGSGMSEAPTSGSLPSLVASQVGPFRLVGTAPVTRLGSSLSPGVVDSIGAVYEGPS
                     GERLNQIILAYASPVVASGRMEIVYQTLATECPGKRLMRSEVRNRDGVVLGTQVVCDQ
                     SPQHVYWNNGRVLTFVTAPHPSALVFYKASKI"
     gene            9346..10098
                     /locus_tag="PK710_09965"
     CDS             9346..10098
                     /locus_tag="PK710_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10085.1"
                     /translation="MSSHRFRYGFHPEGMDPFAIWRRGGYAKGAVIRQARGQASRSDV
                     ISALPPMVSGRSRFRWRIVLLALSAAGLLGGVGVVVHEGLEHSSEAKEADSELIPWVV
                     LEPDEVRTTVVVAHPGPSERAKRGEESSWVPPPLPTKNTDDSLRSSANSAKGPEKEET
                     KDTREQAAEADSGSVVSQVVIDKRKSRAVEVFRATQAESVRGEMLRSAGERDARPEGI
                     RLPLPRTSKEISGLRVGDPVAARGLLAVRRVR"
     gene            10230..>10566
                     /locus_tag="PK710_09970"
     CDS             10230..>10566
                     /locus_tag="PK710_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10086.1"
                     /translation="MSWRANTTRGFMSRMMIGAWLSLAAVGVSDVARAQSEEEVAKAR
                     EQFRLGVQLEAAGDWAGALAQFEAVARVRMTPAVRFHIARCQHHLGSLLEALGGYRLA
                     AHEAEQDPKA"
BASE COUNT         2287 a         2731 c         3308 g         2240 t
ORIGIN      
        1 agatcggcaa gcgcctcacg gtagggcggg tccacatgga cgaccccgag cgtcgaaccc
       61 gattccgagg gttgaatcgg ggttcctttc tgttcgagaa aaggagttcg aatgataccg
      121 atgggttgca tcacgagacg ttcgtcggcc atggggccat tgtctcatga cgagacgaaa
      181 aagtaatgga attcagggag ttccaatcca cgcgaagggt gaagtgtaca ggtcgtcatc
      241 cacggtggcg tcccattcca gcggtgaatt ggactgcgag acgccgggag tgctgctggt
      301 agcagggtcg ttgggaatgc tgtccacagc gctaccgtcg ccgttggcgg cgaagacgcc
      361 gatggtcccc tgatccagcc gatccgcgga aatattcaga agggaaagag ggatctgcaa
      421 ctcgaagaag gtatcgtatt tcgtgtcgtg accagccgtc agataattga tggaaggcat
      481 tccataagcg ccggggttgg tgtcatctgc ctggggatcc acgcccggta gggtatttcc
      541 cacgaaaaac tccccagcca tgcctttcaa tcgaggttcc ttgacaccat cggtcaaagg
      601 aacgagcgcg ctgccatccc aggctcccca atacgttcct tcttgggaga aattgctgtg
      661 gaaatagaat tgatactccg gtttgtccgg tccgacgaag gcgtgatcct tgccccacat
      721 atcaccgcct acggaatagc cacttcccgc cgcggtgtcg aaagccacga attgtacgag
      781 atccatctgg tggatttgcg aactttccga accacctaga ttggcaggat ccacgacatc
      841 ggtgatgttc acgtattgaa tgccgatgta caggaattga tcgtcccacg ccgcatgcag
      901 ggctgcataa tcccagggag gctcatgggc gcaccaattg gtgcccaaaa atctcgcgtc
      961 atccccagcg ggatcccgaa ggagaagggt gtcatctccc cattcattgc cggtgttggc
     1021 cccgtcgatg acgatggttt ttcgagcgcc gtagccgatg cttgctggat tgcccagacc
     1081 tgctccaccg cctccactaa cgcctccccc agttcctccc atcccggcgg tagctcctgt
     1141 gccttgtcct gctgcgccag caacacccgc ggctccccca ccgctacccg cctcatggcc
     1201 tccactacca gcccaggctc cggaacctcc aaccccagcg tcaggccccc ccgcaccaaa
     1261 tcctcccgtg gcaggaggag ggccctccga agaatacgaa ttggaatcat cgtcagaaca
     1321 agcggtggcg acagcgatgg ctgccaatgc ggtaacgaaa aaaagggtat gtgatgacat
     1381 gggtacttcc tcccatctcg agccataccg caaatttttc gacaatcaag ggaatagtaa
     1441 ctcagaactg agtttctacc cccccatgag tttcgcggcc caatcgtgaa gataacgaaa
     1501 cgccaacgcg gccaatagga cggcgaggcc gcccttgatc cagcgttcag gaatgaaacg
     1561 ctgggctctg gcaccgaagt acatgccaaa cacgcctccg acgccgaata agccaccgag
     1621 cagccaatcc ggggcgaccg acatctgacc gtagcaaggc gcgatgagtt ggtagaatac
     1681 gacaccgact acggacgtca cgaaggttcc catcaacgtg gcgccagcca ccgtatgcac
     1741 gggaagttgg tagagggtta cgaaaaaagg agcgagcagg gcgccgccgc caatcccgta
     1801 cactccgccc accattccga ccaccacaga aagcgaaaaa actccaggtg tcgagcaggc
     1861 atgctccttt ccttgaaatc gaatcacgat cttgcgccag gtccacgtct gttcttgcac
     1921 gacgaaaggc tcggcggtgg agcgtgcttt gggggaagcg ccggagaacc tcgtcacgtc
     1981 cttggcgagt cgggcaccga tgaacagcag gacacctccg acaaaggctt tgaaaggggt
     2041 cggatcggga agccaatgca aacggacaaa gcctcccgcg atcaccccgg gcagcgttcc
     2101 cgccaccacg acccaggtca gaggccatac catgcgtcgc tctcgaacga agcgccagac
     2161 cccgcttggg atcgccacca cgttgaatac gagattggtt gggcttaccg acggactcgt
     2221 gaatccgagc acgctcatct gaaagggaag caacacgaac gcgccggaaa cccccgccat
     2281 ggatgtgaag aacgaaacga cgaaggccac caggggaggt atccacaggc tcgtttccac
     2341 gcccgaaacc gaaaaagtat gcagcagcat cgcgaagctc ttgctcccga accttgtatg
     2401 ttattgttat tattatataa tttcgttctc cgaaagacag gtgacggggc catcgccgca
     2461 aatgcgttcg aggcgagagc gcatccgttc ggattgcgcc accgatccat tccacaacat
     2521 ggtcaagggt tcggccatca cattgccgat cacggcacca tgaaaagaag cgatatgcac
     2581 atcgccattg ggttcgatgc agacgtgttc ccatgcggcg cggcatggat gaatcacgct
     2641 tcgattggcg aactggtccg cacccaaaaa cgatgccatc tgtggtgaat ccttcagctt
     2701 gcaccgccag acgcgcgggg gagccgtatg atccaccatg gttagtccaa ggcgacgccc
     2761 ttgtttcact gcctcttcga ccaggtttct ggtttcttcg ccctggggat ccaccaacca
     2821 ttgccgcgca aattccgtgc tggccacgag ttcttcgaac ttgacccaat ccatgccgag
     2881 ccttgcggca agatgcacga tctgcagcaa ttcgaattgg ttctcggtca tcaccacgca
     2941 agacaaccca atcctcaaat cgagctggtg gcggtggcgc agttcgacca tcttttctac
     3001 gtgtttttcg atcacgctca gacgggagcc cacccggatg cggtcgtgaa ccgaaggcga
     3061 ggcgccgtcg atcgagaggg agatggagct aacgccggcc tggacgaggc gttcgacgac
     3121 gtctggcgtg agcagcatgc cattgctcaa cagatgaatg acggtgggag cgcccgcgcg
     3181 tgcgaaccga atggcctcga gaacatcgaa gaaaatggga gcggtaaggc tttctccgcc
     3241 atgcacgaag ccgaagtacg aagcatgagc cagatcttcg cgaatccggt ccaataccgc
     3301 gggggaaagg gttcgcgccg tctgctgttg caccctgtcc ggggaatgat tgatacagtg
     3361 ccggcagcgc acgttacacc gctcggtgat ggaaaaatcg atgcggcttg gacgaacggg
     3421 agcctcagag cggcctgacg caaacgcttc gttgtggcgt cgttttcgtt ccttttccat
     3481 gagttcgatg accccagcag gtcgttggcg cggtttacgc tgagccagca agccggagtt
     3541 ggctccaaga caaacctgct ggtcttgcac gcgaacgtca ccggtggaag ccaccggctc
     3601 gagcagctcg agccaatcat cgtcgagact cagctccacg gaagagtccg aagcattgat
     3661 gacgacgtcc acgatgtcat tgcccagcgc tcggcgtagg acgagcaagc ggtcttcggc
     3721 ataaacgatt tgcatgtctc cgctgtgcag ggcgggatgg ttgcgacgtg cttgcagcag
     3781 cgagcgggtc aacgagcgga aagcgatttg tgtttccgac catgaccacg gcatggggac
     3841 ccggtcttcc caagcgcctt cgggtcgaag agcggcgatt ttcgcggcca ttcccagctc
     3901 ctcgccataa acgagcaggg gagttccggg catggctaca agcatcagca tgcctagggc
     3961 cgtccgattg ggggcacctc gcacggcgca taatgtcgcg aaccgattgt ggtcgtgggt
     4021 ggacaagaag cgcacggcac gattggcact gagcgcttcg ctcacggcat attcgatgcg
     4081 ttctgccgcg gtggccgcgt cgatggaacg ctccgcgagg aaagacgtca tggtcgcgtg
     4141 aaacgaaaaa tccgtggccg catccaccac cccggcttgt tgccatctcc aagcgtgggc
     4201 agggacgact tctccgagca ccaggctatg gggtgcgttt tcacgaagcg tgtcccggat
     4261 acgcgttcct agcgacaggg gaacctgcgc cgccgcatcc agccgaaacc catcgatacc
     4321 gaaacgtgtc cagtgtttga ctgcctggag cgcgagttcc tgcaccggct catgatcgag
     4381 attcaacaaa ggggcatcta ccctgccgcc ataatgatcg agttggaggt ggccggatcg
     4441 atcacgccat cgaaaccacg gagccaaaga ggagccggga ccttcgcgtt ttacctgctc
     4501 atagggggcg aatccggtac ccacatgaag catggaaaaa tcaagcaaaa cacgaagtcc
     4561 tcgctgatgc gccgcatcga gaagacgtcg taaggcttgt tcccctccga tggctggatc
     4621 gaccgtcaac ggatcgatga catcgtagcg atgacaactc gccccaacgt ggatgggggt
     4681 gagatgaaga accgtgacgc caaggtccgc aagggaatcc agggaacgag tgataccgtc
     4741 gatgtgtccg tatgcgggta gatcgggacc gggatcgtct gcccaggccg aatggtccgt
     4801 ttgtggacgg aacctatcga caaatattgt gtatacgaca gcctgttccc agaacgatgg
     4861 cagtctgtct atacatttag gtatatcgaa gtcgtgaccg ttctcttgcc ctacgaaggt
     4921 tccgtcatcg agaagaaaac ggaaacgagc gaagggggta gaggtaggaa gatggctccg
     4981 aaaaacgatg tgttcttgct cctcggaaac ccgcgacatc ggcatgacgg attcggtatc
     5041 cggacgtcct tcggaccagg caatgcgcag actcgagcca aaccctttcc gaagcaaggc
     5101 tgtgatacga tatccaccgc gcggatgagg gacacaatga gggagcgccg gtgcgaatag
     5161 cagcggctcc ggtgttcccg ccacgaccca aacgttgtct cgatgcaccg tacgctgccg
     5221 ggtccttggg ttgttggggt cgagcctcca ggagccatcg accacgagtt tgtaggcgta
     5281 cacgcctgga gcaagatcga tcgcgaactc ccattcgttg ccatccggac ggtgatgcag
     5341 ggggatgggg tcgtggtggc gatcttgaag ttcgccgtgt aattcgacgg aattggcttg
     5401 gaagggcagg gagatacgaa gggggcgggg agtcacggga cctcgaaaaa acgctgataa
     5461 catcatgcgt tgggaaacga ttgtcgaaac ggtttgcatc tgggagcaga atatcctaca
     5521 ccatgagtaa ctcaattatg agttactaat ttgaatgatg tggatgggaa aaacgtgggt
     5581 ggacggttcg agcccacgct ccacaaggag tgcgcgccat gctgaaggtg aaggtcaaga
     5641 agatggggca ggtggtcgtt ggtgccacga gtatgcttgc tttggggcca ttgaccatgg
     5701 gttgtgcgtt ggatgccagt gggcaggaaa cggagcaaca agagacgttg gggcaacaat
     5761 cgcaagccgt ggagccgggg tcggccacgt tgttcaccag tgttcaggcc tacaatcagc
     5821 aagagatttc ggtggtggtg acgaacgcca cgaccgacga tttcattcgt gtgggtgaga
     5881 agctcaagat gagctgtcgt gcggtggatt attggtggtt ggcacatccc aatggttacg
     5941 agggctatga cgacgtcaag aggctcaaga agctcaacat tcaggtaacc gcgcattttt
     6001 atggcaatgg cgtgaagaaa agcagcaaga cggtggccgt gaactcctgg tcgaacacac
     6061 cgcatgcctg ggagttggtg ggaaccacgc aagagttcac ggtgccagcc aacaccgatg
     6121 tgattcgttt ctcttacgct gtcaccgatg gtggcgatgc ttcagtgaac gtgcaggtgg
     6181 gcgaagacaa tacggatcgg gtggtagtat ttggggcacg gcctgaaaag catttggtgt
     6241 tcgagaacga cttttccacg ttgcgtcata agatcatcga ggatggtgcg ctcgttcgcg
     6301 gtcgcgacgt tttggtgggc tatacggact atcgtgcgaa cacgctcgcc gacacgactt
     6361 cgatcaacac ggtcattggg cagcagaaaa actatggccg gtttgggccc gacatcattc
     6421 cgctgcatgg cacgttggaa catgaggtga cgatcggagt gcagatcgat ggggtttggg
     6481 gtcaggaggc ccccatggtg caacgcttcg actccgccat ggttcccagg acgcagatgt
     6541 acaatcgtcg ttttgctttc gaacggcgta tctctttgcc cgcgaacgcg aaacaggttg
     6601 cggtgtattt ccatatcaag acgtacttgc gtgcggatta cagccagttt ccttttgtcg
     6661 tggagaaata cttcgcggat ggggagagga ttttggttcg tgaggtgtgg gacaacaatg
     6721 ggggaagcgg caacgattat catttcggca cggaggcttc gacgcttccc tcggtgcagc
     6781 cgctgcctgc cgatattcga agaacggtgg ttttcgtgca gggcgagacc agtccggggc
     6841 aggacatgtt cgttcgtggt ggggtggatc acggggttgc ggaggctttg cttggcaagt
     6901 catgtacgaa ttcccaaggc gatccgacgt atggttgttc gattccgatc acgaatttga
     6961 accgaaaaaa cgcgtatacg gacccgtgga aggtcggtga tggtcttttg gattggtatg
     7021 gggtcgaggc tgggcagcaa ggcagcgtga atggtcagca agcgaatggt acggcggcgg
     7081 attggacgac caactattgg cccccggagt ggggtccgga aaagactgtg gcgcacgatg
     7141 ggtacgggct cgaggttctg aatcgcgtat gcggcatgca ttgttggatg gtggatgtgg
     7201 acatggattg tgcgaaagcc tatcactcgc cggatggcac accgtggttc gagatcaaga
     7261 cattcatctc gaatggtccg gggtgggaac cggatgtcga gcagggcttg atgaatgcct
     7321 acggaacgcc tgaggcgccg tacgtctcga agaatcatct ggcgcgatgc gggatggtga
     7381 acgtatttcg ccggggacaa tccacggcgg agttctatcg tttcgaccaa ttcttgccgt
     7441 aagcaccatc gattcgtcgc gaccgctttg gtcgtgtagt cccgccaact cgcatccccg
     7501 gcataatttt cgagatgttt ttcctcgatt gacgtgaata cgttttcgcc ccagcgataa
     7561 gtcgttatga tgatgctttc cacgaattgg ggagctgtca tgcagagcga cttgggaacg
     7621 actttgttga cgacatcgtt gatggtggtt tgttatctca tcatcgttcg attggtggat
     7681 atgaacgaga aggagccgct atgggcgatg gcggcattct tcgttcttgg ggctggtgcc
     7741 tcggtggctg tagtgcaggc ggtaggcacg cccatcctcg acttggagcc gctttggggt
     7801 gcggtcatca aggagggagc gcgttttggg gtgcttgggg taggggtggg gggactgttg
     7861 ctctacggca gaaaacgggg atgggatgag ttcaacggac tgctcgatgg cgttgtctac
     7921 ggagcaaccg tgggacttgg gtttgccacc gcgcgggagg tgatgaatca gcttcttgtg
     7981 ggggcctttg cgattccggg agaagaagct ggaatgcttg cgggatttca caaggtattt
     8041 ctatttgggc tagcggatgg tgttttcggg gcaatcgtgg gagccggcat tggcgcggcg
     8101 gtggaggcga gggccatggt gctgcgagtc gtatggccgg tgcttggtgt tgccgcggcc
     8161 atccttgcga attggggata cgtggtcttc ggaaagggca atggtctcgg cggaaacgag
     8221 gggctgattc gagcgcaaat tgctctggtg ttgccggtca tcgtagtcgt agtcgtggtc
     8281 gtttatgcat tgttctccga acgaaaagcc attcgcgttc agctatcgtc ggagcaagca
     8341 gatggcatcg tgacggccgc ggatctggcg cttcttcaaa acgtggtggc gcgtgaaacg
     8401 acctactgga agacgttgtt gtccggaaaa attcgttttt ggagattgat gcgagccttg
     8461 cacaataggc aagttcaact cgcgttcgtc aaacagaatg cctcgcgcga gacgaatgaa
     8521 gagcgaaaaa aaaggctcga ggttgaaata tccaatctca ggctgaatat tcgtgagcag
     8581 caaaccgccc tgggcaaggg gacgaccggc gatgagggga gggaatcatg agtttcatca
     8641 tgaggtactg gcgcattgtc gtttcgaaaa tacctgcatt tttgttgatg gtcgtagcgt
     8701 ttcaggtatc gggttgctgt tgccggggat gttgtaatgc acttccggcg ggcggcagcg
     8761 gatcgtcggg ctctggcatg tcggaggcac caacctccgg gtccctgccc agtctcgtgg
     8821 cttcccaggt agggccattt cgccttgtgg gcaccgctcc tgtgactcgt cttggctcct
     8881 cgttgagccc gggggtcgta gacagcattg gcgcggtata tgagggacct tccggcgaga
     8941 ggctcaacca gatcattctt gcctacgctt cacctgtggt cgccagtgga cgcatggaga
     9001 tcgtgtatca aacccttgcc acggaatgcc caggaaaacg tctcatgcgg tcggaggttc
     9061 gcaaccggga cggtgtcgta ttgggcacgc aggtcgtttg tgatcagtca cctcagcatg
     9121 tgtactggaa caatggtcgt gttctcacgt ttgttaccgc gcctcatcct tccgcgcttg
     9181 ttttttacaa agcctcaaaa atctgagggg aggcataggc tcgcctcggt tccccgaaca
     9241 agttgatcgc aatcgctctg catgattcgc gcgcagcgtc cgtctatcgt tgagcaattc
     9301 ccaaacaatc gacgaagcgt ttttttggat cgtggcaggg cagacatgag ttcccatcgt
     9361 tttcgttatg gctttcatcc ggaggggatg gatcccttcg cgatttggcg acggggagga
     9421 tatgcaaagg gcgcggttat tcgtcaggca cggggtcaag cctcccgaag cgatgttata
     9481 tccgcgttgc ctcccatggt gtccgggcgc tcgagatttc gatggaggat tgtgctgttg
     9541 gcgttgtccg cggctggcct tcttggaggc gtaggggttg tggtccacga ggggctagag
     9601 cattcgtcgg aggcaaaaga agctgattcc gaattgattc cgtgggtggt gttggagccg
     9661 gatgaggttc gaacgacagt cgttgtcgct catcctggac ccagcgaacg tgcgaaacgt
     9721 ggtgaagagt catcgtgggt tccgcctccc ttgccgacga aaaacacgga tgactctttg
     9781 cgatcttcag cgaattccgc aaaagggcct gagaaggagg aaacgaagga tacgcgggag
     9841 caggctgcgg aggctgattc tggcagtgtt gtatcccaag tcgttatcga caagcggaaa
     9901 tcgcgggcgg tggaggtgtt tcgggcaacg caagcggaat cggttcgtgg tgagatgttg
     9961 cggagtgctg gggagcgtga cgcgcggccg gagggtatac ggctgccttt accaagaact
    10021 tccaaggaaa tcagtgggtt acgggtcggc gatccggtgg ccgctcgtgg gttgctggcg
    10081 gttcgacggg ttcggtaggg gcatgaatcg gacttgttga gaatcggagg caatcgttca
    10141 cgacccattc attcgcaaag aatcgttcgt ctgcctggcg taaggggggg cgtcccgtga
    10201 tacactgatg ggataggagg tagcctcgga tgtcctggag agcgaatacg acgcgaggtt
    10261 tcatgagtcg catgatgatt ggggcttggt tgtcgttggc cgcggtaggg gtgagcgatg
    10321 tggcccgggc gcaatcggaa gaagaggttg cgaaagcgcg cgaacaattt cgtttaggtg
    10381 tgcagttaga ggcggccggg gattgggctg gtgccttggc gcaattcgaa gcggtggcgc
    10441 gcgtacggat gacaccggcg gttcggtttc atatcgcgag gtgccaacat caccttggca
    10501 gtttgctcga agctttggga gggtatcgtc ttgctgccca tgaggccgag caggatccga
    10561 aggcac
//
LOCUS       DAOSOR010000147        10563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6283_length_10563_cov_4.368005, whole genome shotgun sequence.
ACCESSION   DAOSOR010000147 DAOSOR010000000
VERSION     DAOSOR010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 10563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10563
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6283_length_10563_cov_4.368005"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PK710_09975"
     CDS             complement(<1..277)
                     /locus_tag="PK710_09975"
                     /inference="COORDINATES: protein motif:HMM:NF015581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HOT10087.1"
                     /translation="MAEPTHLHRLLTVSFSEALERIPEALKGKGFGVLTSIDVAATLK
                     AKIGVDFPSYTILGACNPTMAHRALSIDPTIGVFLPCNIIVRQVQGGV"
     gene            391..1236
                     /locus_tag="PK710_09980"
     CDS             391..1236
                     /locus_tag="PK710_09980"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT10088.1"
                     /translation="MSEKDSASPQIVSPDIIARAQRGEREAVTQLFGDHLGTVHRFAL
                     RMCRDEDRARDIAQESLLTALKSLDSFRGDASFSTWLFTITRSHCGRLRRRSERESVS
                     EDGDVVTDNAASEEPLPDALAAHGEVTSVVEQALDALDPEEREVVLLRDVEGLSTKEA
                     ADALQISVAALKSRLHRARSSLREAVRRVVEPTEFIDQFTDPGCPPIIEAFSKKLEGD
                     LSVADCDSFQQHIASCPACAQRCASIQAVLGACATLRTHPPSTNLQTIIDRTALAIRK
                     QASTE"
     gene            complement(1258..5898)
                     /locus_tag="PK710_09985"
     CDS             complement(1258..5898)
                     /locus_tag="PK710_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10089.1"
                     /translation="MAAQGSPRLSLEAFTVRRVERDPTLGRAPRVHLTLSKRDYPAEG
                     IDPFSPLRLPENVDVCLRDIDSSLLSQASAVMDIELAGGVHRFPLAGRHHFEVEVARG
                     TMAKASVQVGPPPEKPEEEGLLSLWIRGAGDDVVSREGRCVVRSLDISFEPAVQLPNL
                     IPTLLDIQDLFRDRGWSSIMTNPVATRASKIPAVRDVQRWVDLLRAVAPSVNKIGYVL
                     LRHVHAEPVFRERTGRWELTLSFTGHVQFAGNMPAIPFQDVVLPAVLLPIPYASVDDL
                     LSDQPLASADLKKDRVRLDEAIVGMRDLIASWVGHVSLRGQLPHVLVQAKAVDQTDWR
                     VNVTPPAELSVDGRVTGKRQGDTLIVDADDLVIGFPEPGMHLSVRALVEDLGPEQGAW
                     SSRLRVHTENTVASGSVIPRVEIEVDAAHPFATGTSTLDLSLKDLRIDGGAGGLTIAG
                     PNIDLWPMSRRIGFSSDVSTRHDMVVQEVGMRRQLRVPTGRITGALELGADGLWHLDL
                     GVGASVDMSVIKQVPGIPELSIEAGQLVGQLSGDVDVHATAVADFALTNAYRVDFRES
                     KVGALLDRAQVVIEDRRVEFAPGTKIALRAKEASVTSSGVGAMVLDVMWDMHGQPCLL
                     HAGERTASLLATDLRQGELTVHLSPEGRLSFSGERQGLYGIRYFNTLLNPAADPEHLA
                     ELLRSEEALGHVLSALELLSPELADRMSLGRDIVLGLRTLAQRSAIRQLRHFITRPAM
                     ARFLSLVLAGDESQAQRIATQVKNVTEARGIDVKEVKSLLREYLDEFDVDYEIGGIVR
                     WLDVLLGPMEPLDVQPAVALEPLISQPAFQEQLEGYPSAGMIYEQLEAAMTQEQIDTL
                     CRLAPRLTIEQIDYCLRRADTSWGSNQRKWLAFVRAVKRRVERIADAYGGVEYAFQDV
                     VIGTFLGEAVADGLDPAQVSDGAWDDRSHWPPACALGPSDVAVLLKAGLALDRQNRQT
                     QINNRMLIDLMRGRPSRFTLDVFAEIGQHNPRALTGILFAFLEQEQDHLSDPVDLPSL
                     LGDKLGLAVPRRRDFMAGGSRARDSYWKALSELADTILARADASYALKSHLQVKRHEV
                     PVAYRSRSRDKTLIQQARKAIERADAVGMECQFKDKASGPVRKAMALYHDAFDACAQL
                     LQHDRMAFQAPWFKAFWIRNEQALKVLSVVRNYQQDVDRVRPWLHYRTGRNRFRGEQD
                     LLRSVLDVLLYEDKDRKKLERDPLVRLLIDPLPGHLDFTVISAMGVITEGESGAELED
                     AFARLGEQRGVRVIRAPTGTAMSLEENARRIISSIESIEGPYGLMGYSQGCANALAAE
                     SLLRGGTPEQQRLLDRLVCRNLLYSAFNGSAHGVYGSEKFERAMVEGERFLKHYQVLF
                     SSEVVASFLRFARAVVDSPVFVRVLGGVHSLTVQRATDFHRELQIVAHAPTSTLRGVT
                     MEQDLPETLELTYYSLRHMSRGAPQDTQVLASDALGRSTRVINATTRLLERCDMTSMC
                     ERAHHWSPLKKETEFVTTDRDKKRCVYDSPKDRHVFPWIDVNARFGLIRHRE"
     gene            6175..6786
                     /locus_tag="PK710_09990"
     CDS             6175..6786
                     /locus_tag="PK710_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10090.1"
                     /translation="MATNQLLLRMDVPYDHWVLGTVHASCERKQRDCSEFELLRHMRQ
                     AAAASGSTAFVAPRCIDESKGTTAMLCCRATMARALALGDPASSHSDAPQEMRWQVRI
                     AQRRILVIAHPRANRHPPRTDSVFRHDIPPENAIKLGSVHASCSEPCERGDAEKALEI
                     GASGLGANSLSEIRCLEQQAYRWHCQGVASFVQPSPPTNTTSP"
     gene            complement(6773..7183)
                     /locus_tag="PK710_09995"
     CDS             complement(6773..7183)
                     /locus_tag="PK710_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10091.1"
                     /translation="MQVPPGPMGPGTPGMGPPNAQQPGYPPAGTPAAAAGGYGPAPGP
                     FPPGGPSAAGAPGSEGQMKNAKIARGCGFGGCGCALVLLIVGGILFAFGMQRATREAL
                     PFSYVVLGIMPFVGIVGAALLGWGMVTLNKLKGK"
     gene            7325..7741
                     /locus_tag="PK710_10000"
     CDS             7325..7741
                     /locus_tag="PK710_10000"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOT10092.1"
                     /translation="MGSSGYRLIDHTADIALEVWGTSKQLVFEQAAYAIIDQLCEQPL
                     PPSSDSRTVSLVGLDDADLLVRWLNEILWLATEKGFLLTQAIVDVQDQSLHAVIFGLP
                     QAYPLITTEIKSATYHNLQLNQPSEDRWIARVVLDV"
     gene            7969..9963
                     /gene="pepF"
                     /locus_tag="PK710_10005"
     CDS             7969..9963
                     /gene="pepF"
                     /locus_tag="PK710_10005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HOT10093.1"
                     /translation="MRLSSLVAIGLLIGCSLPMGCECPNTSPKPSAIASASSSPPPSS
                     TDRPKGSPPDATMERSQIPDTYKWKLEPIFADDAAFEASLSAMTKDRAMLASYQGKLS
                     KPSELLACLELYFQTRLQTNKLTLYGNLRFDSHQKDTQAQALHEKSLDAMNALMAAAS
                     FIRQEILALDDAAVAKAYAAEPGLSKYKPYVDEVRRRRAHVLGAEAEKVLTLAEDNLW
                     AEIDLNELPSDHEKTFKGLLADIPLPKIHDETNQEVQLTLSSFVKYRGSSDRRVRKEA
                     VDALFGTLHQYRHALAGTLSGQIRRNVFLARARGYNTALEAYLHKDDIDPAVYKNLVS
                     TIHANLAPLHRYMALRKRTMGVDELRIYDLYTPMVETVKTQFTYEQARDVLPKALAPL
                     GEDYLSVLRQGLDPGNGWIDVYPHRDKDSGAFSASVFGLHPYVKMNYFNDVDDMSTLA
                     HEFGHALHSHLSMNNQPYVTASYSMFNAEIASTFNEKLLSDYLIENAKSDAERLYLLN
                     ELVETIRTTIYRQTLFAEFEWLIHTAAEKGTPLTAEFFAKTYESLIRSYYGEHFTLGK
                     DDGMEWAYIPHFYYKYYVFTYATGLSSGIALAQRVLSEGEQARNAYLGMLKGGSSKPP
                     LDLLRDAGVDLSKPDAIAGAAKLLDDTLAKMEKILAAQKK"
     gene            complement(10031..>10563)
                     /locus_tag="PK710_10010"
     CDS             complement(10031..>10563)
                     /locus_tag="PK710_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10094.1"
                     /translation="LVAGLILIVVPLLLWVGPRQPKGSSPAASGSEMVPTAASQEQGD
                     DGLEPESSVDKSSAVELGKVWIDKCEKPGPGKTPPEQCDRQPWFEEALVRAIQENSTC
                     APERAGTVSVVMRVDYRRRQVDVFSGKSGSIRGRGAAGLLACIKRSIPDPNWDSLEHE
                     HTKYIIAVMATYPAKK"
BASE COUNT         2476 a         3577 c         2477 g         2033 t
ORIGIN      
        1 cgaccccccc ctgcacctgg cgaacgatga tgttgcaagg caaaaacacc ccaatcgtgg
       61 gatcgatcga caacgcccga tgagccatgg ttgggttaca agcccccaaa atcgtgtagg
      121 aagggaaatc aaccccaatc ttcgccttga gcgtcgcggc cacatcaatg ctggtcaaca
      181 cgccaaagcc cttccccttg agcgcttccg gaatgcgttc gagcgcttcg gaaaaactca
      241 cggtaaggag tcggtgcaaa tgagtcggct cagccatggg aaacactcct gggaatcagc
      301 gaaaatattc gcatgaacat agtcatcaac aacggtgcat agcccgccaa ccttgcgctt
      361 ggcatcggtt ggatgcaagt gagagaactc atgagtgaaa aggattccgc aagcccacaa
      421 atcgtcagcc ctgacatcat tgcccgagcg caacgaggcg agcgcgaagc ggtaacccag
      481 ctattcggtg atcacctggg aacggtgcat cgtttcgccc ttcggatgtg ccgagacgaa
      541 gatcgcgctc gcgacatcgc ccaagaaagc ctgctcaccg ctctgaagtc cctggatagt
      601 tttcgaggcg acgcctcttt cagcacatgg ctgttcacca tcacacgaag tcattgcggt
      661 cgcttacgac gacgttccga acgagaatcc gtgagcgaag atggggatgt ggtgaccgat
      721 aacgccgcct cagaagaacc tttgcccgat gcgttggccg ctcacggcga agtcacttcc
      781 gtcgtggaac aagcgctcga tgccctcgat cccgaagaac gggaagtcgt gttgctacgc
      841 gatgtcgaag gactttccac gaaagaagcc gctgatgccc tgcaaatttc cgtcgcagcc
      901 ctgaaaagtc gccttcaccg cgcccgctcg tcgctccgag aagccgtccg ccgcgtcgta
      961 gaacccacgg aattcattga tcaattcacc gatcccggct gtccgcccat catcgaggcc
     1021 ttctccaaaa aactcgaagg cgacctttcc gtcgccgatt gcgattcgtt ccaacaacat
     1081 atcgcttctt gccccgcctg cgcacagcga tgcgcgtcca tccaagccgt cttgggtgca
     1141 tgcgctacct tgcgaaccca ccctccctcg accaacctgc aaaccatcat cgaccgcacc
     1201 gccctcgcca tccgtaagca agcctcgacc gaataactcc ccccaactca gaaaatgtca
     1261 ttcgcgatgc cggatcagac caaagcgcgc attgacgtcg atccacggaa aaacatgacg
     1321 atctttcggt gagtcgtaca cacaccgctt tttgtcacgg tccgtcgtaa cgaattccgt
     1381 ctcctttttg agcggcgacc aatggtgcgc ccgctcacac atcgacgtca tatcgcaacg
     1441 ttcaagtaac cgcgtggtgg cattaatcac gcgcgtggat cgccccaacg catcactcgc
     1501 aagtacttgc gtgtcctggg gagccccacg ggacatatga cgcaacgagt aatatgtgag
     1561 ttccaacgtt tcagggagat cttgctccat cgtcacgcca cgaagcgtcg aggtaggagc
     1621 atgggcaacg atctgcaact cccgatgaaa gtcggtggcc cgctgcaccg taagggaatg
     1681 tactcctccc aatacacgta cgaatacggg cgaatccacc acggcccgcg cgaaacgaag
     1741 aaacgatgcc acgacctccg acgaaaatag cacctgataa tgcttgagaa accgctcacc
     1801 ctcgaccatg gcccgctcaa atttttcgga accatacacg ccatgagccg acccgttgaa
     1861 cgcactgtac aaaagattac ggcacaccaa tcggtcgaga agcctctgtt gttcgggtgt
     1921 tcctcctcgc aacagactct ccgcagcaag tgcgttggca cacccctggc tataccccat
     1981 gagcccgtaa ggaccctcga tcgattcgat gctcgagatg atccgcctgg cgttttcttc
     2041 caaactcatc gccgttcccg taggcgcgcg aatcacccga acccctcgct gctcgcccaa
     2101 tcgcgcgaaa gcatcctcca attccgcccc ggattctcct tccgtaatga cgcccatcgc
     2161 gctgatgacc gtaaagtcca aatgccccgg caacggatcg atgagcaatc gtacgagcgg
     2221 atcacgctcg agcttctttc ggtccttgtc ttcgtacaac agaacgtcaa gcaccgatcg
     2281 aagcaaatct tgctctcccc ggaatcggtt gcgtcctgtc ctataatgca accagggccg
     2341 caccctatcg acatcctgct gatagttgcg caccaccgac aacactttga gcgcttgctc
     2401 gttgcgtatc caaaatgctt tgaaccaagg cgcctgaaac gccatcctgt cgtgctgaag
     2461 aagctgcgca caagcatcaa atgcatcatg gtagagcgcc atggctttgc gtactggacc
     2521 gctcgccttg tctttgaatt ggcattccat ccccacggca tccgcgcgct cgatcgcttt
     2581 tctagcctgt tgtatgagcg tcttgtcccg actccgtgac cgatacgcca cgggtacctc
     2641 atgccgcttg acctgcaggt gcgacttcaa agcgtaggac gcatccgcac gcgcgagaat
     2701 ggtatccgcc aattccgaca atgccttcca ataactgtct ctcgcacggc ttcccccagc
     2761 catgaaatca cgccgacgcg gcaccgccag gcccagcttg tctcccagca acgacggcaa
     2821 atctaccgga tcggatagat gatcctgctc ttgttccaga aaggcaaaga gaatgcccgt
     2881 caacgctcgc ggattgtgct gcccaatctc cgcgaacacg tcgagcgtga aacgcgatgg
     2941 ccgaccccgc atcaaatcga tcaacatccg attgttgatc tgcgtttgcc ggttctgtcg
     3001 gtcgagcgcc aatcccgctt tcaacagcac cgccacatcc gaaggtccca acgcacaggc
     3061 cggcggccag tgggatcgat catcccacgc accatcgctc acctgcgcgg ggtccaaccc
     3121 gtcggccacc gcttccccca aaaatgtccc aatcaccaca tcctgaaatg catactccac
     3181 ccccccatac gcatcggcga ttctctccac gcgtcgtttg accgcgcgaa caaacgccaa
     3241 ccacttgcgt tgattcgaac cccacgacgt gtccgcacgt cgcaaacaat agtcgatttg
     3301 ttcgatggtg agccgtggtg ccaagcgaca gagcgtgtcg atctgctctt gcgtcatggc
     3361 tgcttcgagt tgctcgtaaa tcatcccagc gcttggatag ccttccaatt gctcttgaaa
     3421 ggccggctgc gatatcaacg gctctagcgc tacggcaggt tgcacatcga gcggctccat
     3481 cggccccaac agaacgtcca gccaacgaac gatgcctccg atttcatagt cgacatcgaa
     3541 ttcatccaag tactccctga gcagactctt gacttctttg acatcgatcc cccgcgcctc
     3601 ggtcacgttc ttgacctgcg tggcaatccg ttgcgcctgc gattcatcgc ccgcaagcac
     3661 aagggacaga aatcgcgcca tggcaggacg tgtaatgaaa tgccgcaatt gtcgaatggc
     3721 ggaacgctgc gcaagcgttc gcaatcccaa tacgatatcg cgcccaagcg acatccggtc
     3781 cgccagctcc ggactcaaca actccagggc cgacaacaca tgcccaaggg cttcttccga
     3841 tcgcaatagc tccgcaagat gctccggatc cgccgcggga ttcaatagcg tgttgaagta
     3901 ccgaatccca tacaatccct gcctttctcc actgaaagac aatcgcccct ccgggctcaa
     3961 atgaaccgtc aactcacctt gcctcaaatc cgtcgccaac agcgatgccg ttctctcacc
     4021 cgcatgcagc aaacacggtt gcccatgcat gtcccacatc acgtccagca ccatcgcccc
     4081 aacccccgaa gaagtcacag aagcctcttt ggctcgcaaa gcaatcttcg ttcccggagc
     4141 aaattcgacc cttcggtcct caatcaccac ctgcgcacga tccaacaacg cccccacctt
     4201 cgattcgcga aaatcaacac gatacgcatt ggtcaacgca aaatccgcaa ctgccgtcgc
     4261 atgaacatcc acatcgccac taagctgccc caccaattgc cccgcttcga tcgatagctc
     4321 cgggatcccc ggaacttgct tgatcacact catgtccacc gaagcaccaa cccccaaatc
     4381 aagatgccac aacccatcgg cgccaagctc aagggcaccc gtgatgcgcc ccgtcggaac
     4441 ccgaagctgc cttcgcatcc ctacctcttg caccaccatg tcgtgacgcg tgctcacgtc
     4501 ggaagaaaat ccgatgcgac gactcatcgg ccacaaatca atgtttggac ccgcaatcgt
     4561 caatccaccc gccccaccat cgatccgaag gtctttcaag gacaaatcca atgtcgaagt
     4621 tcccgtggca aaaggatgtg ccgcatcaac ctcgatttcg acccgcggaa tcaccgaacc
     4681 cgatgccacc gtattttccg tatgcacgcg caatcgcgaa gaccacgcac cttgctccgg
     4741 acccaggtcc tcgacgaggg cccgaaccga tagatgcatg cccggttcgg gaaaaccaat
     4801 gacaagatcg tccgcatcca cgatcaaagt atcgccttgc ctctttccag tcactcgacc
     4861 atccaccgaa agctccgcag gcggcgtcac attcactctc caatccgttt gatccacggc
     4921 tttcgcttgg acgagcacat gcggaagctg ccctcgcaac gacacatgcc ccacccagga
     4981 ggcaataagg tcacgcatcc ccacgatcgc ttcgtcgaga cgaacacgat cctttttcaa
     5041 atccgccgag gcaagcggtt gatccgaaag caaatcatcc accgacgcat aggggattgg
     5101 caatagaacc gcgggaagca ccacatcttg aaacggaatc gcgggcatgt tgcccgcgaa
     5161 ttgcacatgc cccgtaaaac tcagcgtaag ctcccatcga cccgttcgct cacgaaatac
     5221 cggctctgcg tgaacgtgcc gaagcaatac gtaaccaatc ttgttgaccg atggtgccac
     5281 ggcacgaagc aaatccaccc atcgctgaac atctcgcacc gcaggtatct tcgacgcacg
     5341 agtcgcaacc ggattggtca tgatcgatga ccacccccga tcccgaaata aatcctgaat
     5401 gtcgagaagc gtcggaatca gattcggaag ctgtaccgcg ggctcaaacg aaatatccaa
     5461 agaacgcacc acacaccgcc cctcacgcga taccacgtcg tccccagcac cacggatcca
     5521 caacgataac aacccctctt cttccggctt ttctggcggt ggacccacct gaacactcgc
     5581 cttcgccatc gtccctcgtg ccacctccac ctcgaaatga tgacgccccg ccaaaggaaa
     5641 tcgatgaact ccacccgcca actcgatgtc catcaccgct gaagcctgag aaagcagcga
     5701 tgaatcgatg tcccgtaaac agacgtcgac attctccgga agacgaagag gcgaaaacgg
     5761 atcgattcct tccgccggat aatcgcgttt cgaaagcgtc agatgtaccc gaggcgctcg
     5821 tcccagggtc ggatcccgct cgactcgacg aaccgtaaat gcttccaacg aaaggcgcgg
     5881 tgaaccctgg gctgccatga tgagactcac gatacacacg tgtccgcacg ggtccactcc
     5941 ccattgtaga ccccgtcgcc attgcactcg gccccacgaa tgcactgtga tacttggaac
     6001 acatcaggcg aatctcacgc accccgacga aagacccgga aattcgatat ttattcaatg
     6061 atttcaatac gatacattat tttcggggca gcgttggccg aactcccttt cctgagcctc
     6121 gcctgctcca cccacactca cgtgcgcgtc gaaggccatc gcctccccgc cgccgtggcg
     6181 acgaaccaat tgctcctgcg catggacgtt ccctacgacc attgggtcct gggaaccgtc
     6241 catgcctctt gcgaacgaaa gcaacgagat tgctccgaat tcgaactgct ccgacacatg
     6301 cgacaagccg ccgcagcgtc gggaagcacc gccttcgtag cccctcgatg tatcgatgaa
     6361 tcaaaaggga caacggccat gctttgctgc cgcgctacga tggcacgagc tttggcgctg
     6421 ggggatcccg cttcgtctca ctccgatgca ccccaggaaa tgcgatggca agtgcgtatc
     6481 gcccagcgtc gcatcctggt gatcgcgcac ccacgtgcaa accgacatcc tccacgcacc
     6541 gactcggttt ttcgacacga cattcccccc gaaaacgcca tcaagctcgg gtccgtacac
     6601 gcttcgtgct cggagccctg cgaacgcggg gatgcggaaa aagcattgga aataggagcc
     6661 tccggtctgg gcgcgaactc gctatccgag atccgttgtc tcgaacagca agcctaccgt
     6721 tggcattgtc agggcgtcgc gtcgttcgtc caaccttccc ctccgacgaa cactacttcc
     6781 ccttgagctt gttgagggtc accatgcccc aaccaaggag cgccgcgccc acgatgccaa
     6841 cgaacggcat gattcccaga acgacatacg aaaacggcaa agcttctcgc gtggcccgct
     6901 gcataccgaa cgcaaatagg atgccgccga caatcagcag aaccagggca cacccgcacc
     6961 ccccgaagcc acaacccctc gcaatttttg catttttcat ctgcccttcg gaaccagggg
     7021 ctcccgcggc tgacggtccc cccggaggaa agggcccagg tgctggtcca tagccgcccg
     7081 ctgccgcggc cggtgtgccg gctggaggat aacccggttg ttgcgcgttc ggtgggccca
     7141 tccctggcgt tccaggtccc atcggtccag gaggtacttg catgaccgcc cgtatcgaat
     7201 ggtccaaggc ggatgtcaat cggttcacac ttccgccccg ctcgatttcc ctgccccaac
     7261 ggaatgtcca acacggtagc cactgaccat cgcgcccggc gcgcaataga agtacgaaaa
     7321 cctcatgggt tccagcggct accgcctcat agaccatacc gccgacatcg ccctcgaagt
     7381 ctgggggacc tccaaacaac tcgtattcga acaagctgcc tacgccatca tcgatcagct
     7441 ctgtgaacaa ccacttcccc catcctccga cagccgcacc gtaagcctcg tcggcctcga
     7501 cgacgcagac ttgctggtgc gatggctcaa cgaaattcta tggcttgcca ccgaaaaagg
     7561 ctttttgctc acacaagcca tcgtcgacgt acaagaccaa tcgcttcacg ccgtgatttt
     7621 tggcctcccc caggcctacc ctctcatcac gacagagata aaaagcgcca cgtaccacaa
     7681 cctgcagctc aaccaaccat ccgaggaccg ctggatagca cgcgtcgttc tcgacgtttg
     7741 atcttcctca ttccctcgtc cgcgacaatg ccgcgtgttc ccgtcaccgc gatcgcctcg
     7801 ctgccaccat gcagtcgcaa gcgcttgaaa tcagcaatca ttttcgaaac atgcccccgt
     7861 cagaccccca ccgatcccgt cgggatgacg aacccgcttc aatcctgtag ggtctctcgc
     7921 agcgccagaa cgcaacctcg cctcgcgcga atatccaagg atccaaccat gcgtctgtct
     7981 tctctcgtcg ccattgggtt gctcatcggc tgctcgctcc cgatgggttg cgaatgcccc
     8041 aatacatccc ccaagccttc ggcaatcgcc tcggcttcct cctcgcctcc accatcgtcg
     8101 acggacaggc cgaaagggtc cccgccggac gctaccatgg aaaggtccca aattccggat
     8161 acttataagt ggaagctcga acctattttt gccgatgatg ccgccttcga agcttccctt
     8221 tccgcgatga ccaaagaccg agccatgctc gcctcgtacc aaggcaagct gtcaaaaccg
     8281 tcggagttgc tggcttgcct cgagctgtat ttccaaacac ggcttcaaac caacaagctg
     8341 accctgtacg gcaacttgcg tttcgactcg caccaaaaag acacccaggc ccaggccctg
     8401 cacgaaaaat ccctcgatgc catgaacgca ctcatggccg ccgcctcctt cattcgccag
     8461 gaaattctcg ctctcgacga tgccgctgtc gccaaagcgt atgcggcgga accaggtcta
     8521 tcgaaataca aaccctatgt ggatgaagtg cgacgtcgta gagcccacgt tctcggcgcc
     8581 gaagccgaaa aggtgctgac cctcgcggaa gacaacctgt gggctgagat cgacctcaac
     8641 gagctaccct ccgaccacga aaaaaccttc aagggcctgc tcgcggatat ccctttgccg
     8701 aaaatccacg acgaaaccaa tcaagaggtg caactcaccc tgtccagctt cgtcaagtac
     8761 cgaggctctt cggatcgacg cgttcgcaag gaagccgtcg atgcgttgtt cggcacgcta
     8821 caccagtatc gacacgccct cgcgggaacc ctatccggac agatccgacg aaacgtgttt
     8881 ttggctcgcg ccagaggata taacacggcc ctcgaagcct acttgcacaa agacgatatc
     8941 gatccggccg tttacaaaaa cctcgtctcg accatccacg ccaacctcgc cccgctgcat
     9001 cgatacatgg cattgcgaaa acgaaccatg ggagtcgatg aattgcgaat ctacgacctg
     9061 tatactccca tggtcgaaac cgtgaaaacg cagttcacgt atgagcaagc gcgcgacgtt
     9121 ctccccaaag cgctcgctcc cctcggagaa gactatctat ccgtcctgcg acaaggcctc
     9181 gaccctggca atggttggat cgacgtctat ccacatcggg ataaagacag cggcgctttc
     9241 tctgccagcg tcttcgggct ccatccgtac gtgaagatga actacttcaa tgacgtcgat
     9301 gatatgtcca cgctggccca cgaattcgga cacgccttgc acagccacct ttcgatgaac
     9361 aatcagccct acgtgactgc cagctactcc atgttcaacg cggaaatcgc ctcgaccttc
     9421 aacgaaaaac tgctgtccga ctacctcatc gaaaacgcaa aatcagacgc agaacggctc
     9481 tatcttctca acgaactcgt cgaaaccatc cgtaccacca tctatcggca aaccttgttt
     9541 gccgaattcg aatggctcat ccataccgct gccgaaaaag gcacacccct gaccgccgaa
     9601 ttcttcgcca aaacctacga atcgctcatc cgctcctact acggggaaca cttcaccctc
     9661 ggcaaagacg acggcatgga gtgggcttat atccctcact tctattataa atactacgtg
     9721 ttcacctacg cgaccggcct gtcctcggga atcgcgctcg cccaacgcgt tctttccgaa
     9781 ggcgaacagg ctcgaaacgc ttaccttggc atgctgaaag gcggatcgtc caaacctccg
     9841 ctcgacttgc ttcgggatgc cggcgtcgac ctgagcaagc ccgatgccat cgcgggcgcc
     9901 gccaaactgc tcgacgatac cctcgccaaa atggaaaaga tcctcgccgc acaaaagaaa
     9961 tgacaccgcc tgcctgtctc gaggcacgac ccgcctgtcg atactcgtgc tgccacccac
    10021 ggctgcaaca ctacttcttg gccggatagg tcgccatcac cgcgatgatg tacttggtat
    10081 gctcgtgctc gagggaatcc cagttcggat cgggaatcga acgcttgatg caagcgagca
    10141 accccgccgc cccacggcct cggatggaac cacttttccc tgaaaataca tctacttgcc
    10201 tacgacggta atccacacgc atcaccacgc ttaccgtccc tgcccgctcg ggagcacaag
    10261 tgctgttctc ctgaatggcc cgcaccaatg cctcttcgaa ccacggctga cggtcacatt
    10321 gctcaggcgg tgtcttgccc ggtccaggct tctcacactt gtcaatccat actttgccca
    10381 attcgaccgc cgaagactta tccacgctcg actccggctc caacccatcg tccccttgtt
    10441 cctgcgaagc cgccgtcgga accatttccg aaccggaggc cgcgggagaa gagccctttg
    10501 gttgacgagg acccacccac aacaacaaag gaacgacgat gaggatgagt cccgcaacca
    10561 gcg
//
LOCUS       DAOSOR010000148        10499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6328_length_10499_cov_4.581099, whole genome shotgun sequence.
ACCESSION   DAOSOR010000148 DAOSOR010000000
VERSION     DAOSOR010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 10499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10499
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6328_length_10499_cov_4.581099"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /gene="pyk"
                     /locus_tag="PK710_10015"
     CDS             complement(<1..903)
                     /gene="pyk"
                     /locus_tag="PK710_10015"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOT10095.1"
                     /translation="MERAGQKTKIVATIGPASDSPQILRAMVDAGMDVVRLNFSHGDP
                     DYHATVISRVREVAKAADARITILGDLPGPKMRIGKLASEPIELIRGNRFTLTTESIV
                     GDETRVSFSFPQLPSAVKAGDKLYLNDGFILLVVEQVRGNEIQCVVEVGGELRSNKGL
                     NVPGIDLGISAFTDNDHAWLRFAASHGVDAVSQSFVASAQDIQTLRNAAQALDYHPFV
                     IAKIERACVLDRIEEVLLASDGVMVARGDLGVEIPIERIAVVQKQITDLANRHAKPVI
                     TATQMLESMVSFPRPTRAEATDVAN"
     gene            1373..1744
                     /locus_tag="PK710_10020"
     CDS             1373..1744
                     /locus_tag="PK710_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10096.1"
                     /translation="MRKRPRVRIEVSDPSFLNGRWHCVGKILGTKSERLPWLGSDPPP
                     DEPEDEPLTATLYLVAGAGASPEEAKRQVMAKIHNVWGSESTPPPGPCILESDAATRN
                     NSRQGSSFVSRFMNMLRRRTG"
     gene            2002..3555
                     /gene="pyrB"
                     /locus_tag="PK710_10025"
     CDS             2002..3555
                     /gene="pyrB"
                     /locus_tag="PK710_10025"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOT10097.1"
                     /translation="MAEHTKFFGKNWVDVSSVTRSELEYFLKLSATLKEAIREQRVNE
                     YKLARDRDLFAALLFFEPSTRTRFSFEIAALRLGLRPVGFSGTEATSVKKGESLLDTV
                     DMFDAYDIDAVILRHPLDGAAKAVTEHLVRDEARPLCCFNGGDGQNEHPTQGLLDVFT
                     IQECCGRLHDLEIGIAVDPKYGRTTHSFPVLLSLFPNNRFHVFSHPDLRMPKAVIQHL
                     ESRGVRVTEYFEEPDALKEMLPRLDFLYMTRLQRERFEDDAEYYAARDMFLFTPEMMA
                     RTKTNFGVGHPLPDNKEFPTIHPSLKTHRKYWPKRQAGNGVPTRLTEMALSLGLAGFD
                     FDGKCHRPRTPATDCVRDRDPGSHKNRNGSMDIRPVVNGTVIDHVEGNPYVIQKISKL
                     LKLCERGDIFRMGVVEPIKRPGTRKGVLMIKDRLLEEQDVRLIATIAPGATVNDIHDG
                     RVVRKRDLFLPEIVEGLPGTHCTNHRCITRAEYHEHVPVKAVRVSPEEKNIVKCFYCN
                     NLMHSDELF"
     gene            complement(3750..5324)
                     /locus_tag="PK710_10030"
     CDS             complement(3750..5324)
                     /locus_tag="PK710_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOT10098.1"
                     /translation="MPSAVFVHDAQRTPSYRNCGLDVVTLLSEAARPIVERYRRSIDC
                     LLVSCQDPSSFNQVNHLAAAICDNLGLVGIEATRVENASNSIGVVTLACKAALCGSNV
                     LVIGGELMNDPSVSPGRREEIIAQVVPAKDRAYGVTMPAAVALITSYFAGYHGIGADR
                     LRRILEEIAVLSYHAASFNRYAQLRGEKFRKDNVRKNYRSERNRPLCEPLHFYDVCPT
                     TDGSGAILLSCNPVLEQRKARVKITGHALVNDFNRIIHRNRLEALAASEIAAQRAYAM
                     AGIPVEYRNDGTFYLPEYPPGFFAEIHDAFGPLYLLNLLNLGMYGLAQAEDAILSGKT
                     DPTSADCLLPINPSGGLKDGHPVGGTGLIKMVETYWQLVGQPNGLDLRKAPFPFEPRT
                     AVVHSVGGPGSVNGVVVMEHVDNDPRPAIWPTRIPPVTAAKRTFQNADFPKPGRFVAS
                     TRRHFYAAAGSRSTPPAESIDIGMVELWEDGKPGPRKLAIPMDDRSLTFGDGVRVWKS
                     YRDGRYHFERDYPDRH"
     gene            5506..6006
                     /locus_tag="PK710_10035"
     CDS             5506..6006
                     /locus_tag="PK710_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10099.1"
                     /translation="MRYYVSLKNREIPVDVTLVPGGGFDVRIDDRRVDADMVEVSGGY
                     SVRVGDRVLDVAIEGKPPELGIVAGGYRGFVVVESERQKAVSAARRGRLDSQQNVVIS
                     PMPGRIVKVFVEAGQEIAVGEPVIVVEAMKMENELRAPRSGQVKRVLVSVGERVEGNA
                     TLIELT"
     gene            6098..6895
                     /locus_tag="PK710_10040"
     CDS             6098..6895
                     /locus_tag="PK710_10040"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10100.1"
                     /translation="MDTASEIIGNKDWARTALRLIGMAAIVFVLDAVLDAYRLASLLL
                     GLFAIEFLARKQGFQWDETGKRTTMQSTGVVALGFGLGAFVVLSMVGVAWLAGYAQVS
                     IGSPTMVGLGLGLVTPVVQAARDELLFRAAPLTLLKKHVPDRFALPFVALLGSAPLWL
                     HPTPSIVGIGLTIVTGWIFALAIRMGKGILLAWGMHAGWLFMLGAGIRGSVFDVSLGS
                     STWLPFAQAQGAAAWIALVVVVLLAVGFSAYYLRTRKVADCPTVPIP"
     gene            6981..8570
                     /locus_tag="PK710_10045"
     CDS             6981..8570
                     /locus_tag="PK710_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT10101.1"
                     /translation="MRRFVAAAACLSFLALTSESSAILGPSAAHHARGTENVFWFLHV
                     SDVHIGSPLYGNHGKHANWLFDEAIGIVQPWFVVVTGDLVDAAKNKVPTAGQVQAEWD
                     EYRSLYEGAVMTPSFYFDLPGNHDGYGDEGMGHYLANSLLGRTHNRLYTAWTVEIPAG
                     QYLFFGFNSAGNGSGPFFEKPAFTTDEIEVFQQTVLNHDSARQVFALAHHPLSKPAKG
                     KEVEDELKSLGGAYYLHGHDHDYEEYLAGGGTVVVNQVASLGAKDTNNVGVGVVDHDG
                     FVYRATNTKSPWPFVVITAPMGVDLRNGGKKHPYAYNVCKDRNDNPVRALVFSLTPPK
                     QVRVKIGNGAEVSMTPSVSSSNLWTANVDTSKLTTGVHDVMVTADVDGASADHKIRTR
                     FIDGPCDPLPEDPPPVEQDAGVDAQPDGDAGDSDGGNSDADDGAAGASGDAQPDGAGA
                     MGGSGGVEGGTSGAGGTNVAGQGGDADSGVTETQKSDDYAASGGGCSCTTVEQTPLSR
                     TGNVWLMALMASVWWRWRRRG"
     gene            complement(8665..>10499)
                     /locus_tag="PK710_10050"
     CDS             complement(8665..>10499)
                     /locus_tag="PK710_10050"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT10102.1"
                     /translation="LVGLGEIDKARALAQEALQQAPQHVGARILIAELDIKAGQEEKA
                     ATALSQIVGPKADPAIHKAASRAELVSIHTLNGQVHLARSRMSAAEEAFAEALKLDPK
                     DANALCGFGEVLYREGRFAEALARFEAGMQADAENVAARIGAAKARIALERLQEAKDI
                     LRKLRETRPSDPVVAYWLAKAEEALGNKPEVEKILTDAIGRDPKGVDAINLYIALAQF
                     LAAQGRTQEADEKLIEARRKLPDSAAIHKALGTVALAAGRLNEAKSEFELALQQDSSD
                     LSSLFSLAVSLRRMGRFDEAQEKFNSISTIDKNYPGLALERGVLFEASGQAQRALEMY
                     QEALSKAPNDPDLMLRVGSAEVASGHATQAEDILRKVLRARPNSAEANHYLGRAQLLK
                     GTNLAEALRFLRRAAEIDPNRAEYWLYIGWAANEAGQPGTAEKALEKALELDQSLADA
                     YWQRGVLLRRQGTIKDAERDLLKALELKPSRFEAYATLAECYEDQSKWGPANTAWRKA
                     IEADSNRPFWRYKLGRLLYNAHDRGGAAEQLKSAVQLAAKETRPAWLWEAYLMLADVS
                     KTAGRRAEAIEYYQEFLKLAPPDSPFRADAAKQLAALGASADGR"
BASE COUNT         2211 a         2845 c         3096 g         2347 t
ORIGIN      
        1 gttggctaca tccgtggctt ccgcgcgggt cggccggggg aaagaaacca tcgattcgag
       61 catttgcgtg gccgtgatga caggtttcgc gtgtcgattg gcaagatcgg taatctgttt
      121 ttgcacgacg gcaatgcgtt cgatgggaat ctcgacaccg agatcaccgc gcgccaccat
      181 cacgccatcg gaggccagaa ggacttcttc aatccgatcg agcacgcatg ccctttcgat
      241 ttttgcgatg acgaagggat gataatccag cgcttgggcg gcgtttctga gcgtttgaat
      301 atcctgcgcg ctggccacga atgactggct taccgcatcg actccgtgag aggccgcaaa
      361 acgtaaccac gcgtgatcgt tatccgtaaa agcggatatg cccaggtcga ttcccggtac
      421 attcaacccc ttgttggaac gcagttctcc gccgacttcc acgacacatt gaatctcgtt
      481 gccccgaacc tgttccacga ccaagaggat gaacccatcg ttgagatata acttgtcacc
      541 ggctttcacc gcggacggca attgcggaaa gctgaagctt acgcgtgtct catcgccaac
      601 gatgctttcg gtcgtcagcg taaaacgatt gcctcgaatc aattcgatgg gctctgaggc
      661 tagtttgcca atccgcattt tgggtcccgg aaggtctcca agaatcgtga tgcgagcatc
      721 ggccgctttc gccacctctc gcaccctcga gatgaccgtg gcgtgatagt ccgggtcgcc
      781 atgcgagaag ttgaggcgaa ccacatccat gcccgcatcc accatggcgc gaaggatttg
      841 gggagaatcc gaagccgggc cgatcgtcgc aacgattttc gtcttttgtc ctgctcgttc
      901 catgattcgc tgaactgtag gtcataccta aaaaaaggtg gggggatttc cacgccatcc
      961 accgccaaat caccgttttc accaccttgg gcggattcaa tccacgggca agcgactcca
     1021 gaaaatggac ttgctgcagg agtcacgctt ctttgctcgc gtgatcggcc cttgcgaaac
     1081 ctggaagaac cgtaacgagg gagggagagg gaaaatagga gccctattcg tgccgaaaac
     1141 tccccaaaaa tcgcgttcaa tgattcgaaa tcgacgcttc gaggcgaatc attttttggc
     1201 atcgtggcat cgaaatagaa aaagcaaatc gttcgggaat ggagcttgtg aaagatcggg
     1261 cctcatccca ggattgaact gactcctcta cccttggttg tcacgatgac tcgtgctagc
     1321 cagcccagtg attatgtcga gtcgaaagcc aagtgagcca ccggaaagca tggtgcgaaa
     1381 gcgtccccgc gtacgcattg aagtgtcgga tccgtcgttc ctcaatggtc ggtggcattg
     1441 tgtgggaaag atcctcggga cgaaatccga acggctgccg tggcttggct ccgacccacc
     1501 cccagatgag ccagaagacg aaccgcttac cgcaactctt tatctcgtcg ccggcgcagg
     1561 agcctcacct gaagaagcaa aacgacaggt gatggccaag atccataatg tctggggctc
     1621 ggaatccaca ccgccaccag gcccttgcat tctcgagtcc gatgccgcca ctcgaaacaa
     1681 ctcccggcaa ggatcgtcgt tcgtttcccg attcatgaac atgctgcggc gacgcacggg
     1741 gtgatctgac ccctacgccg ctgccatcca cgcccaatcg agtcgtccag catcaccagc
     1801 ccgccaccgt agcgtcctca cttcaaagat cgagtgaaac gaaatcaaga taggatgaag
     1861 gtgaatttat ccatcgtcac gactcgcaaa acaaaaaaat gttcaggtga tcgtcggctg
     1921 agcttggcct tctgccattc gaacgtctat ggcgacgcca acacggagcc gatggtggcc
     1981 ctgcgtaagc tgggaggatc tatggctgag cataccaagt tctttggaaa gaattgggtg
     2041 gatgtatcgt ccgtgacgcg ctcggaactc gagtacttct tgaagctttc ggcgaccttg
     2101 aaagaagcca ttcgcgagca gcgcgtgaat gaatacaaac tggctcgcga tcgagatcta
     2161 ttcgcggcgc tgcttttttt cgaaccctcc acacgcacgc gcttcagttt cgaaatcgcc
     2221 gcccttcgcc tcggtctgcg ccccgtgggc ttttccggca ccgaagccac ctccgtcaaa
     2281 aaaggagaat ccctgctcga caccgtcgat atgttcgatg cctacgatat cgacgccgtg
     2341 attttacgtc atcccctcga tggtgccgcg aaagcggtga ccgaacacct ggtgcgtgat
     2401 gaggccagac ccttgtgttg tttcaacggc ggcgacgggc agaacgaaca cccaacgcaa
     2461 gggttgctcg acgtattcac cattcaggaa tgctgcggac gtcttcatga cctggaaatc
     2521 ggaattgccg tcgacccgaa atatggccga accacgcata gtttccccgt cttgctgagt
     2581 ctatttccga acaaccgctt tcacgtgttc agccatcccg accttcggat gcccaaagcg
     2641 gtgattcaac atctcgaaag cagaggcgta cgagtcaccg agtacttcga ggagcccgac
     2701 gctctcaaag aaatgcttcc tcgactcgat ttcctctaca tgacgcgatt gcagcgcgag
     2761 cggttcgagg acgatgccga gtattatgcg gcgcgggaca tgtttttatt caccccggag
     2821 atgatggccc gcaccaaaac gaactttggc gtggggcatc cgttgccgga taacaaagag
     2881 tttcccacga ttcatccttc cctcaaaaca catcgaaagt attggccgaa acgacaagca
     2941 ggaaacggtg tccccacacg tcttaccgaa atggcgctct ccttgggtct cgctggattc
     3001 gacttcgatg gcaaatgtca tcgtccacgc actccagcaa cggattgtgt cagggatcga
     3061 gatccgggaa gccataaaaa ccgcaacggt tcgatggata tcagacccgt cgtcaatggt
     3121 accgtcatcg atcacgtgga aggcaatccc tacgtcatcc aaaaaatcag caaactgttg
     3181 aagctctgcg aacgaggcga catttttcga atgggtgtgg tggaacccat caaacgtcct
     3241 ggaacacgca aaggcgtgct catgatcaaa gaccgattgc tcgaggagca ggatgtgcgc
     3301 ctcatcgcca cgattgcgcc tggcgcaacg gtcaacgaca ttcacgacgg tcgcgtggtg
     3361 cgaaaacgtg acctatttct tccggaaatc gtcgagggat tgccgggaac ccattgtacc
     3421 aaccatcgat gtatcacccg cgcggagtat cacgaacacg ttcccgtcaa agccgtgcgc
     3481 gtgagcccag aagaaaagaa catcgtgaag tgcttttatt gcaacaacct catgcatagc
     3541 gatgagttgt tttgaatcaa tattggttga tatccgcgct tgtggcgtgc catcgactcg
     3601 cgagctgctt ttctcatggc ctcgataggg agtgccttca cgctacggcc aacgcatgac
     3661 ttcacaaccg attcgatcgc cgatcctatc catgctcctc ctccttcgaa gcaaggggcc
     3721 aagcccgata tgcttggatt cagcggggat caatgtcgat ccgggtagtc tcgctcgaag
     3781 tggtatcgac catcgcgata gcttttccat acacgcacgc catcaccaaa cgtaagggag
     3841 cgatcgtcca tcggaatcgc aagtttgcgt ggtccgggct tgccgtcttc ccaaagctcc
     3901 accatgccga tgtcgatgga ctcggcaggt ggcgtcgaac gggagcccgc ggccgcgtag
     3961 aagtgccttc gcgtggatgc cacgaagcgt ccgggtttcg ggaagtcggc gttttggaaa
     4021 gtgcgcttgg cggcggtgac gggaggaatg cgcgtgggcc aaatggcagg acgagggtcg
     4081 ttgtcgacat gctccatgac gaccacgccg ttgacgctgc cgggaccgcc gacgctatgg
     4141 accactgcag ttctcggttc gaaggggaag ggggcttttc gcaagtccag gccattgggc
     4201 tgaccgacaa gttgccaata ggtttccacc attttgatga gccctgtgcc gccgacggga
     4261 tgaccatctt ttaggcctcc cgagggattg atcggcagca ggcaatcggc ggaagtcgga
     4321 tccgttttgc cggagaggat ggcgtcttct gcttgcgcaa gaccgtacat cccgaggttg
     4381 agcaagttta aaaggtaaag cggtccgaaa gcatcgtgga tttcggcgaa aaatcctggt
     4441 ggatattccg gcaggtaaaa ggtgccatcg ttgcggtatt cgacgggaat accggccatg
     4501 gcataggctc gttgcgcggc aatttccgaa gcggccaggg cttcgagccg attgcggtga
     4561 atgatgcgat tgaaatcatt gaccagcgcg tgccctgtga tcttcacgcg cgctttgcgt
     4621 tgttccaaca cgggattgca actcagcaaa atcgcgcccg acccatcggt cgtggggcag
     4681 acatcgtaaa aatgaagggg ttcgcacaag ggacggttgc gttcgctgcg ataatttttt
     4741 cgaacgttgt ccttgcgaaa tttctccccc cgcaattgtg cgtatcggtt gaaggaggcg
     4801 gcatgatagg acaacaccgc gatctcttcg agaatacgac gcaatcgatc cgcgccaatc
     4861 ccgtgatatc ccgcgaaata actcgtgatc agcgcaacgg ctgccggcat cgtgacgcca
     4921 taggcgcgat ctttcgcggg caccacctgc gcaatgattt cctctcgtcg ccctgggctg
     4981 acggaaggat cgttcatcaa ctcgccaccg atgacgagca cgttcgagcc acacagcgcg
     5041 gctttgcagg cgagggtgac gacaccaatg ctgttcgatg cattttcgac gcgggtggct
     5101 tcgatgccca cgagccccaa attgtcacaa atggcagcgg ccaagtgatt cacttgattg
     5161 aacgaactgg ggtcttgaca gctcacgagc aggcaatcga tgcttcgacg ataacgttcc
     5221 acaatgggac gggcggcttc ggacaacagc gtgacgacat cgagtccgca attccgatac
     5281 gaaggcgtgc gctgagcgtc gtgaacgaat acggcagacg gcatacctca gtgtaaaccg
     5341 caacgggtgc cgggtagata tcgaatcgtc ttgggccgga tccttgtcgt tccaacccct
     5401 tacccaaacg atgtttcacg ccgcgaggac atcgcgccat cgaaccgtgt tttcaacgcg
     5461 cccacgcttg tcctgtgcca gcagtcttgg tagcgtctcg ggccgatgcg atattatgtc
     5521 agcttgaaaa accgagagat accggtagac gtcacactcg ttcctggcgg agggttcgac
     5581 gttcgcatcg acgatcgcag ggtcgacgcc gacatggtcg aggtcagcgg cgggtattcc
     5641 gtacgcgtag gcgatcgcgt tctcgatgtg gccatcgaag gaaaaccccc ggaactcgga
     5701 atcgtcgcgg gaggatatcg aggatttgtc gttgtggaat cggaacggca aaaagccgtt
     5761 tccgcggctc gtcgaggccg gttggactcc cagcagaatg tggtcataag tcccatgccc
     5821 ggacgaatcg tgaaggtttt tgtagaagca ggacaagaga ttgccgtcgg ggaacccgtg
     5881 attgtggtcg aagccatgaa gatggaaaac gaacttcgcg caccacgctc cggtcaggtg
     5941 aaacgagtgc tggtttctgt gggcgaacgg gtagaaggca acgcaaccct aatcgaattg
     6001 acttgaagat gggagtgttc cgtgtctcca aaacgacgcg cggcgtgggc acgatgaaac
     6061 ggattgggtt ctgacggctc ttacggttcg acgcaagatg gacacggcat cggaaatcat
     6121 tggcaacaag gattgggcgc gcacggcgtt gcggctgata ggaatggccg cgatcgtttt
     6181 cgttctcgac gccgtgctcg acgcttatcg gttggcatcg ctgctgctgg gactgtttgc
     6241 cattgagttt ttagcccgaa aacagggatt tcaatgggat gaaacgggca agcgaacgac
     6301 gatgcaatcg acgggtgtcg ttgctttggg atttggactc ggcgcttttg tcgtcttatc
     6361 aatggtgggg gtggcgtggc tggccggcta cgcccaagtg tcgatcggtt ctcccacgat
     6421 ggtcggactt gggcttggat tggtgacgcc cgtggtgcaa gccgcgagag atgaattgct
     6481 attccgtgcg gctccgctga cgttgctgaa aaagcatgtt ccggaccggt ttgctcttcc
     6541 gttcgtagcc ttgctcggct ccgccccctt atggcttcat cccacgcctt ccatcgtcgg
     6601 aatagggttg accatcgtga caggctggat tttcgctctc gcgattcgaa tgggaaaagg
     6661 cattcttctc gcgtggggta tgcacgctgg ctggctattc atgctcggtg ccggaattcg
     6721 cggctccgtg ttcgacgtgt cgctcggctc gtcgacctgg cttccttttg ctcaagccca
     6781 aggggcagcc gcctggatcg cgttggtcgt cgttgtcctg cttgcggtcg gtttttcagc
     6841 ttattacctg cgtacacgaa aggtagccga ttgtcctacc gtgccgattc catgatgaaa
     6901 tcctcatcga tcgcgcatgt tctcttaggc tccgtgctac gataacgagt tggaccagag
     6961 ctgcatatcc aggagccatc atgcgtcgat tcgttgctgc tgctgcttgt ctgtcgttcc
     7021 ttgccttgac ctcggagtct tctgccatcc ttggtccttc cgcggcgcat catgcgcggg
     7081 gaacggaaaa cgtattctgg ttccttcatg tctcggacgt tcatatcgga agcccgttgt
     7141 atgggaacca tggcaaacac gccaactggc ttttcgacga agcgatcggg attgtacagc
     7201 cctggtttgt tgtggtgacc ggggacttgg tggatgcggc gaaaaacaaa gtacccacgg
     7261 ccggtcaggt gcaagcggag tgggatgaat atcgctcact gtacgaaggc gctgtcatga
     7321 cgccttcttt ttacttcgac ctgccgggga atcatgacgg ctatggcgat gagggaatgg
     7381 ggcattacct ggcgaattcg ttgctcgggc gaacgcataa ccgcttgtat acggcttgga
     7441 cggtagagat accggccggt cagtatttgt tctttggatt caacagcgcg ggcaatggaa
     7501 gcggaccgtt cttcgaaaag cctgccttta cgactgatga aatcgaggtg ttccaacaga
     7561 cggtgctcaa ccatgattcc gcccgacagg tgttcgcgct cgcgcatcat cctctcagca
     7621 agccggccaa gggaaaagaa gtggaagacg aactcaagtc gttgggggga gcgtattatc
     7681 tacacggtca cgatcatgat tacgaagagt acttggcggg tggcggaacg gttgtggtca
     7741 atcaagtcgc atccttgggt gccaaggata ccaacaacgt aggggtgggt gtcgtcgatc
     7801 acgatgggtt cgtgtatcgc gccacgaata cgaaaagtcc atggccgttc gtggtgatca
     7861 cggcgccgat gggcgttgac cttcgcaatg gcggaaaaaa gcatccgtat gcctataacg
     7921 tgtgcaaaga ccggaacgac aatccggtgc gagcactcgt cttttcgctc acgccgccga
     7981 agcaagtgcg ggtgaaaatc ggcaacggcg cggaagtatc gatgacgcct tctgtgtcat
     8041 cgtcgaattt gtggacggca aacgtggata ccagcaagct cacgactggt gttcacgacg
     8101 tcatggtgac ggcggacgtg gatggtgcct cggccgatca taaaattcga acgaggttta
     8161 tcgatggtcc ttgtgatccg cttccggaag acccgccgcc ggtggagcaa gatgccggcg
     8221 tggatgcgca gcctgatggg gatgcgggcg attctgatgg gggcaattcc gatgcggacg
     8281 atggggcggc gggtgcgagc ggtgatgctc aaccagatgg cgcgggggcc atgggcggaa
     8341 gtggaggcgt ggaaggggga acctcaggtg ccggaggaac gaatgtggca gggcagggcg
     8401 gtgatgcgga ctccggtgta acggagactc aaaaatccga tgactatgcg gcaagtggag
     8461 ggggatgctc ctgcacgacg gtggagcaaa ccccgttgtc tcgaacgggc aacgtgtggc
     8521 tcatggcttt gatggcgagc gtgtggtgga gatggcggcg ccgcggatga tcggcgcttg
     8581 atgcattgat tcggtgggaa gctggtgcga acgttgacga gctgtcgcga actgccttgc
     8641 gagtagtcga agcgatggcc gatgctagcg gccgtcggca gaggcaccga gggcggcgag
     8701 ttgtttggcg gcgtcggcgc ggaaggggga gtcggggggc gccagtttta gaaattcctg
     8761 atagtattca atagcttcgg cgcgtctccc cgcggttttg ctcacatccg ccagcatgag
     8821 atacgcttcc cacaaccagg ctgggcgggt ttcttttgca gcgagttgga cggctgattt
     8881 tagctgttct gcggcgccgc cgcgatcgtg agcgttgtac agcagtcggc cgagtttgta
     8941 tcgccagaag ggtcgattgc tatcggcttc gatggctttt cgccacgcgg tattggcggg
     9001 gccccatttg gattgatctt catagcattc agccaacgtg gcgtacgcct cgaagcgtga
     9061 gggtttcagt tcgagggctt tgagcagatc gcgctcagcg tctttgatgg tgccttgtcg
     9121 tcgcagcagc acgcctcgtt gccaataggc atccgcgagg ctttgatcca attccaatgc
     9181 tttttccaaa gctttttccg ctgtgccggg ttgtcccgcc tcgttggcgg cccatccgat
     9241 atagagccag tactcggcgc gattgggatc gatttcggcc gcacgtcgga ggaaccgcag
     9301 ggcttcggcg agattggtgc ctttgagaag ctgtgcgcgt ccaaggtaat ggttcgcttc
     9361 cgcgctgttg ggtcgtgccc gcagcacttt gcgaagaatg tcctcggctt gtgtcgcatg
     9421 accggaggcc acttcggcag aaccgacccg cagcatcaag tcgggatcgt tgggcgcctt
     9481 cgacaatgcc tcttggtaca tttccaaggc tctttgcgcc tgtcccgatg cttcgaagag
     9541 cacgccccgt tcaagggcaa gcccaggata attcttatcg atggtcgaga tgctattgaa
     9601 cttctcctgt gcctcatcga accgtcccat gcgtcgcaaa ctcaccgcca agctaaacag
     9661 ggaggacaag tccgaggagt cttgctgcag agcgagttcg aattcgctct tcgcttcgtt
     9721 caatcttccg gccgcaagtg ccacggttcc caaagctttg tgaattgccg cggaatccgg
     9781 caacttacgc cttgcctcga tgagtttttc gtcggcttct tgtgtgcgtc cttgagcggc
     9841 aagaaactgc gcgagggcga tgtacaagtt gatggcatcg acgcctttgg ggtctcgtcc
     9901 aatggcatcc gtaagaattt tttcgacttc gggtttgttg cccaaggctt cttcggcttt
     9961 ggcgagccaa taggcaacca cgggatcgct ggggcgagtc tctctcagtt ttcgcaggat
    10021 gtctttggct tcttggagcc gttcgagggc gattcgtgct ttggcggcgc caattctggc
    10081 agccacgttc tcggcatcgg cttgcattcc cgcttcgaac cgcgcaaggg cttcggcgaa
    10141 gcgaccttcg cgatacagca cttcgccaaa cccacacaat gcattggcat ctttgggatc
    10201 gagtttcagc gcctctgcga aagcttcttc ggcagcggac atgcgtgaac gcgcgagatg
    10261 gacttgtccg ttgagcgtgt ggatcgacac caattcggcg cgcgaagcgg ctttgtggat
    10321 ggcggggtcg gctttgggac cgacaatctg cgacaaggcg gtggccgctt tctcttcctg
    10381 ccctgccttg atatcgagtt ccgcgatcaa aatgcgggct cccacgtgct gaggggcctg
    10441 ttgcaacgcc tcttgcgcca acgcccgcgc tttatcgatt tcgccaagcc ctaccaaag
//
LOCUS       DAOSOR010000149        10491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6335_length_10491_cov_4.296953, whole genome shotgun sequence.
ACCESSION   DAOSOR010000149 DAOSOR010000000
VERSION     DAOSOR010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 10491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10491
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6335_length_10491_cov_4.296953"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..2780
                     /locus_tag="PK710_10055"
     CDS             165..2780
                     /locus_tag="PK710_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10103.1"
                     /translation="MLLTCLALVGLSSCATSPGSDGADSDNLLAHRSAVDSSGVSRPG
                     RLTDGRAAEEGDDWRTQLTAVLRSRDAYVVYDLGQSMPIVAVSLQGDANDTYSLFVSD
                     DGQSYELLWAARPVRGSGMQTRTATGLEGRGRYVKLQASGGDGRYSVGELGVWSRVPD
                     PFPPTLRTSKGKPSQEDAEQRMQIFGLVAAVFLLMHRRDSPWWMKALVIVPVGFGISL
                     GLKLSELWPLEQAELTLLRATIAAVAMVAVIRQYFPGKAPADSRWTTATLGLLAVLAF
                     GCYYNFGMPQFRDEAKGRQTLVHPWDMRVYFPLVKYFRELRFDGLYLASLAAYVDNNP
                     NVGESGIAHTRLRDLNNNEVKVAAEVMPDIRAVRDRFTPERWESFRKDMKYFQDLMGA
                     GGYLGSLRDHGGNATPVWILSATPFWAWSPANEWTLSLTALLDPLLLLAMLVVVGRTF
                     GWRVAFLLAIGFGTTDLSRFGTNLMGSTLRLDWMVALGFGACALYKKRWMAGGALLAY
                     AGLIRGFPAFATLFLAAPFAMAFVDWARTKDRPAWREGLREAFPPFLRSAAGAIACVL
                     VLVALSSAVFGYQASWGNWFYKISIHQDKPNVNHVGLRNILSYESDRTGKQVLRPELP
                     EPWTDWQKYQLAAFDRRKPLFYGGILLFTGLAVAASRRRHYHQAAMMGLLMIPVFFYP
                     ANYYCHYVFLLPLLAVDRKDTDSRLFGWVALVLLAMSVALYPTLQERQVDVLYTLQSD
                     VILAGFFLILVPMAYFAWRSWPNALAVADGPSLFEESSDEGQGSSSGRKKTKRRKKAK
                     DRAMGEAGSTKAKGASEAEEASESVASEAAASEAEEASESVASEAAASEAEEASRETE
                     TTEEVSETKEGEKSD"
     gene            2849..3931
                     /locus_tag="PK710_10060"
     CDS             2849..3931
                     /locus_tag="PK710_10060"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOT10104.1"
                     /translation="MDASIVDTHLLDPHSLAGQLGVSLEAVQLAHQVELIDLHVDSFI
                     PYRLYGYDWFERHRSGPLGRLFVGHVDVPRALEGGLSGAMWSITTNPFRTARGRWRTF
                     VRNLHRLRAIFDRSGGKLMLVRSYGEYIAARKKGAIASMIAIQGGHALEAAPNGVASI
                     PDEAVVRVTLVHLTSSAVGVTSVPTSILCRHKGLTDRGRELVRQLDAARCFVDLAHIH
                     PKGFWDAVEEHDRSLPLIVTHTGVSGVKPHWRNLDDTQVRAIADSGGTIGIIFHPGFL
                     RAPGAARDRTVIIDHMQHVIDTVGEDHVSIGSDFDGAIIPSKDLASAGAYPRLVQSML
                     DRGWETARIEKVLGANFLRVFKMMRP"
     gene            4133..8788
                     /locus_tag="PK710_10065"
     CDS             4133..8788
                     /locus_tag="PK710_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOT10105.1"
                     /translation="MLSRKRYWMLALCAVPWVVGCAGGESTDSPAGGSGGKGGSGGTG
                     GSAGTGGSSASGGSGGSTDACVPITCEEKSAECGTISDGCGSTIDCGSCTAPETCGAD
                     NRCSCTPITCASAGANCGTLSDGCGNEIDCGQCTAPETCGGSGTPNVCGEGTCTPTTC
                     EDEGAECGTISNGCDGTLDCGSCTAPETCGGGGEDNICGCTPTTCSAVGANCGSVPNG
                     CGGTLDCGSCTAPETCGGGGTPNVCGKPCVPTTCDALGFNCGSASDGCGGTLDCGSCT
                     APETCGGGGTPNVCGKPLPVPGAGDLVITELMYNPKNTPLEDPFEWFEVYNPGITTYS
                     LQGCVLSECIRSHTISTVLTIGPGEFVTFASSAQPGFTPDYVHNRDVILTNSGGSMTI
                     TCGSVEVDKVQYKTSSPWPASTDGFSIALDPAHFNATDNDDGANWCLATQAYGAQGNH
                     GTPGVTNDSCACKPLTACPTGLNCGDYPDGCGGTLSCGTCSGSETCGGGGTPNVCGQG
                     PCVPTTCVAEGAECGSISDGCGKTLDCGACDAPETCGGGGQDNICGCTPTTCTILGLN
                     CGPVPDGCGGTLDCGTCAAPLTCGGGGEDNVCGCTPLTACPAGLNCGTISNGCGGTIS
                     CGTCTTPETCGGGGVPNVCSLPHPEAGQVIFSEIMPNPKTLDDKDGEWFELHNPTTSA
                     FNLRDCTIKDNSATFTIGADLNIQAGGYLVFARSSSPGFTPDMVFTGLELANTDDQLT
                     LTCASVMIDEVKYTSAFPFGDGVAMNLDPTHFNATDNDLAANWCKATESYNGDRGTPG
                     KANTSCGCTPLTACPSGLNCGDYNDGCGGTISCGTCIAPQTCGGGGTPNVCGCTPLTA
                     CPSGLNCGDYNDGCGGTISCGTCIAPQTCGGGGTPNVCGCTPLTACPSGLNCGDYNDG
                     CGGTISCGTCTASQTCGGGGEDNVCGLPPPDVGQVIFSEIMVDPTTVGDTGGEWFELH
                     NPTTSAFDLKGCVVRDNSGSFTINTSLIIPSGGYLALANGDSPGFTPAYVYSGIALAN
                     GGDELHLTCASNLIDEVIFTSGFPIQAGKALNLSPDSLTATANDDPANWCIASVSYNG
                     DLGTPGAANSSCGSAPTYTIGWCNLQFPPKMVSIQGEKRDVYGRVFIDGLTNQGSGPN
                     PNSQVIGQVGFGSNDSDPTTWTNWTAATANDGFSDPANDEYMATVTIPTASATHYDYA
                     FRFSGDGGNTWKYCDLDGSDNGYAPAQAGDLLSKTSHSIDWCRLQTASISGAEGTNAT
                     AYGQLYIEGLTDLNKTGNDPHPIVKGQVGYGPDASTPPNSWTWIDADPNPGWTPSDSN
                     NDEYMATFPIPAASGSPYDLAFRFSGDDGATWTYCDLDGSTTGGYTTDQAGEITVTPV
                     APATLFFSEYIEGSSNNKAVEIYNPTGADVDISQCSVRVYSNAASSPSKTITPPASTP
                     HLASGGVFVICHNSAVSGIKDKCDYQTASLDFNGDDAVELVCNGTTLDVIGQIGFDPG
                     TAWGSGNTSTVDHTLVRKCGIQSGDTDGSDPFDPAVEWDGYSQDTFTYLGSHTLTCP"
     gene            complement(9034..>10491)
                     /locus_tag="PK710_10070"
     CDS             complement(9034..>10491)
                     /locus_tag="PK710_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10106.1"
                     /translation="LTVGFFAWLIRPSRMGATRSAVAAGAVAGIFILVLQNWVDFSLE
                     MPGVAISAVALIGTCWGDTRRRGVARSLGHPWASRFGRRGLTVGFAVLGGLALLLVAT
                     RGMRTALDDRLEFRRLIEGPRMSKDRFNPLIRAAMLRHPAEPYIPLVGAERAWRERDA
                     NPIPYLQRVFTRAKVYGRAHLLLAEILFARHARHQALMELKFACRDEPALTTTAVGLA
                     VHYKVLGKDLDRMIPEGDDGLIVMDTLAGWMHTRDPETSQRYDEELLKRDPARVGPRI
                     RQVINRIHALKNAENPLCEGEAKRRACADQIEEHIAQIEKSEPDTSRAARLRADALMA
                     LHQPEKADKILAAACDHVKDRNECLRARVPIVVALGHESELETLLDAVAASGCSSPAA
                     CAQTHLWIGRTQLARKNLGAATSAFQRATRHDPRNVAAWTALGNTSSALGLHAQAARA
                     YEQALVLDPQNEDLRKKLDKEKRDAALSISPSRPF"
BASE COUNT         1988 a         2951 c         3317 g         2235 t
ORIGIN      
        1 cggttgcgat ggaccctact tccaagcgtt ccgtcggtcg atggacaacg atgggcttat
       61 cggggttcat cgctgcttgc gagcgggttc cggtcaatgc cagactgggc gccatgtcgt
      121 gcttctggca gcgagcgttc gtccgacatg gttggacatg gttcatgctc cttacttgcc
      181 ttgctctcgt cggcttgtcg tcgtgcgcga ccagccctgg ttccgacggg gcggattccg
      241 ataaccttct tgctcatcgt tccgcggtgg attcctcggg tgtgtctcga cccgggaggc
      301 tgaccgatgg tcgtgcggcg gaggaaggcg atgattggcg tacgcaactc accgcggtgt
      361 tgcgaagccg cgatgcgtat gtggtgtacg acctggggca atcgatgccg attgtcgcgg
      421 tgagtttgca gggtgatgcc aacgatacgt actcgttatt cgtatcggac gatgggcaat
      481 cgtatgaatt gttgtgggcg gcgcgtccgg ttcgcggttc ggggatgcaa acgcgcacgg
      541 cgacggggtt ggagggtcgt gggcgttatg tgaaactcca ggcgtcgggc ggtgacggtc
      601 gatatagcgt gggggaactg ggagtttggt cccgcgtgcc cgatcctttt ccgccgacgc
      661 tacgaacgag caaaggcaag ccatcgcagg aagatgccga gcagcgcatg cagatttttg
      721 ggctggtggc cgcagttttt ttgttgatgc atcgtcggga ttcaccttgg tggatgaagg
      781 cgctcgtgat tgttcccgtg gggtttggga tttccctggg gctcaagttg tcggaattgt
      841 ggccgttgga gcaggcagaa ctcacgctat tgcgcgccac gattgcggcg gtggcgatgg
      901 tggcggtgat tcgtcaatat tttccgggaa aagcacccgc cgattcgcgg tggaccaccg
      961 ctacgttggg gcttttggcc gtgcttgcct tcggctgtta ttacaacttc ggcatgccgc
     1021 aatttcggga tgaggccaaa gggcgtcaga cccttgttca tccatgggat atgcgggtgt
     1081 acttcccctt ggtgaagtac tttcgcgagc tgcgattcga tggtttgtat ctcgcgagcc
     1141 tggccgccta tgtggacaac aatccgaacg tgggtgaatc gggaattgcg catacgcgtt
     1201 tgcgggattt gaacaacaac gaggtgaaag tagcggccga ggtcatgccg gatatccgtg
     1261 cggtacgcga tcgcttcacc ccggaacgct gggaatcctt ccgcaaggat atgaagtact
     1321 tccaagatct gatgggggcg gggggatacc tgggaagtct gcgtgatcac gggggcaatg
     1381 ccacgcccgt gtggattttg agcgccacac cgttttgggc ctggtctccc gccaatgaat
     1441 ggaccctttc tctgacggct ttattggatc ctttgctgtt attggcgatg ctggtcgtgg
     1501 tgggaagaac ctttgggtgg cgggtggcct tccttcttgc gattggtttt ggcacgacgg
     1561 atctatcgcg gttcggtacg aatctgatgg gctccacgct gcgtctcgat tggatggtgg
     1621 cgctggggtt tggagcttgt gcgctttaca aaaagcgttg gatggccggc ggagcgctgt
     1681 tggcttacgc gggattgatc cgtggtttcc ctgctttcgc cacccttttt ctcgcggctc
     1741 cctttgcgat ggctttcgtg gattgggcga gaaccaaaga tcgtcccgcc tggcgagagg
     1801 ggcttcgcga agcgttccct cctttccttc gctcggccgc gggggccatc gcctgcgtgc
     1861 tcgtgctcgt cgccctatct tctgccgtgt ttggctacca ggcgtcgtgg ggcaattggt
     1921 tctataaaat ctcgattcac caggacaaac ccaacgtcaa ccacgtgggg cttcggaata
     1981 tcctctccta cgaatcggat cggacgggaa agcaagttct tcggccggaa ctacccgagc
     2041 cctggacgga ttggcaaaag taccagctcg ccgcgtttga tcgtcgtaag ccattgtttt
     2101 atggtggtat tttgttgttt acgggtttgg ccgtcgccgc cagtcggcgc cgtcattatc
     2161 accaagccgc catgatgggt ctgttgatga tccccgtgtt cttttatcca gccaactact
     2221 attgccatta cgtgtttttg ctcccgcttc tggcggtgga tagaaaagac acggattcac
     2281 ggctgtttgg ctgggtagcg ctcgtgctgc tcgcgatgtc cgtggcgctt tatcccacgc
     2341 tccaagaacg acaggtagac gtgctgtaca cgctgcagag cgacgtgatc ctggcaggtt
     2401 ttttcctgat tctcgtgccg atggcgtact ttgcctggcg gtcttggccc aatgcgctcg
     2461 cggttgccga tgggccatcg ttgtttgagg aatcatcgga cgaagggcaa ggctcctcga
     2521 gcgggcgtaa gaagacgaag cgacgcaaga aggcgaaaga ccgggcgatg ggcgaggcag
     2581 gctcgaccaa ggcgaaaggg gcctcggaag cggaagaggc atcggaatca gtggcctcgg
     2641 aagcggcggc ctcggaagcg gaagaggcat cggaatcagt ggcctcggaa gcggcggcct
     2701 cggaagcgga agaggcatcg agggaaaccg agaccacgga agaggtttcg gaaacgaagg
     2761 agggggaaaa gagcgattga gttgaggatg agggggcggg tgttcatccg cggttcgaac
     2821 gccacccatg gtctgctatg gatgggctgt ggacgcatca attgtcgata cgcatcttct
     2881 cgacccccat tctttggcgg gtcaacttgg cgtttccctc gaagccgtac agctcgcgca
     2941 tcaggtggaa ctcatcgacc tgcacgtgga ttcgttcatt ccgtatcgtc tgtacggcta
     3001 cgattggttt gaacgtcatc gttcaggtcc gctagggagg ctgtttgtgg gccacgtgga
     3061 cgtgcctcgg gcgctcgagg gcggactgtc gggggcgatg tggtccatca cgacgaatcc
     3121 gtttcgcacg gcgcgaggcc gatggcggac gttcgttcgg aatttgcatc gtcttcgggc
     3181 cattttcgat cgctccgggg gcaaattgat gctggttcgc tcctatgggg aatacatagc
     3241 cgcgaggaaa aagggagcga ttgcttccat gatcgccatt caaggggggc atgcgctcga
     3301 agcggcaccc aacggtgtgg catccatccc ggatgaagcc gtcgttcggg tcacgctggt
     3361 ccacttgacg agttccgcgg tgggagtgac gtcggtgccc acgtcgatct tgtgtcgtca
     3421 caaggggctg acggatcggg gtcgtgaact cgttcggcag ctcgatgcgg cccgatgttt
     3481 cgtggacctg gcgcatattc atcccaaggg tttttgggat gcggtggaag agcatgatcg
     3541 aagcttgccg ttgatcgtga cccatacggg agtgagcgga gtcaagcccc attggcgcaa
     3601 cctggacgac acccaggtgc gtgcgattgc cgacagtggc ggaacgattg gaatcatttt
     3661 tcatccggga tttctacgtg cgcccggtgc cgcgcgcgac agaacggtca tcatcgacca
     3721 tatgcaacac gtcatcgata cggtggggga ggatcatgta tcgatcggta gtgatttcga
     3781 tggtgcgatc attccctcga aggatcttgc gagcgcggga gcgtatccgc gattggttca
     3841 atcgatgttg gatcgaggat gggaaaccgc gcggatcgag aaggtgctgg gcgccaattt
     3901 cttacgtgtt ttcaagatga tgcgtcctta gggcggaagg gtgagcctcg atggaggcgt
     3961 cttggggtgc gtggggagcc aggcgggttg agatcgtgag agtcagggtt ccggtcacga
     4021 actgaagaac gcggttttcc caaggctcaa acgagggttg ggcgggatta ttcgttatcc
     4081 cattgccgtt tcccgtgcta atccatgggg aaaccacgcc cgggaggcga aaatgttgag
     4141 cagaaaacgg tattggatgt tggcactgtg tgcggtgcct tgggtggttg gctgtgcggg
     4201 aggcgagtcc acggacagtc ccgcgggcgg cagcggtggc aaaggaggca gcggtggaac
     4261 cggtggcagc gctgggactg gtggcagtag cgcatcggga gggtccgggg gaagcacgga
     4321 cgcctgcgtg cccatcacct gcgaggaaaa aagcgctgaa tgcggcacca tttccgatgg
     4381 gtgtggctcg acgatcgatt gtggaagctg cacggcgccg gagacctgcg gtgcggataa
     4441 ccgatgctcc tgtacgccca tcacgtgtgc atcggcggga gccaattgtg gaacgctgtc
     4501 cgacggttgt ggaaacgaga tcgactgcgg gcaatgcacg gcgccggaga cctgcggcgg
     4561 aagtggaacg cccaacgttt gtggtgaagg cacctgcact cccacgacgt gtgaagatga
     4621 aggggcggag tgtggaacca tctccaacgg atgtgatggg acactcgatt gcggaagctg
     4681 cacggcaccg gagacgtgcg gcggcggtgg ggaggacaat atttgtggat gtacgcctac
     4741 cacgtgcagc gcggtaggcg ccaactgcgg ttcggtgccc aacggttgcg gtggcactct
     4801 tgattgcgga agctgtacgg cgccggagac ctgcggtggt ggcggcacgc ccaatgtctg
     4861 tggaaagcct tgtgttccca ccacgtgcga cgcgctaggt ttcaactgcg gttcggcgtc
     4921 cgatgggtgt ggtggcacac tcgattgcgg aagctgtacg gccccggaga cctgcggtgg
     4981 tggcggcacg cccaatgtct gtggaaagcc tttgcctgtg ccgggcgctg gggacctcgt
     5041 catcaccgaa ctcatgtaca accccaaaaa tacgcctttg gaagatccct tcgaatggtt
     5101 cgaagtgtat aacccgggta tcaccacgta ttctttgcaa gggtgcgtgc tgtccgagtg
     5161 catacgctcg catacgatat ccacggtgct caccatcggg ccgggggagt tcgtcacctt
     5221 cgcaagcagt gcccagcctg gatttacccc ggattatgtc cataaccgcg acgtcatcct
     5281 taccaactcc ggcggaagca tgaccatcac ctgcggatcc gtcgaagtcg acaaagtgca
     5341 atacaagaca tcgtcgcctt ggcccgccag caccgatggc ttttccatcg ctctcgatcc
     5401 cgctcatttc aacgccacgg acaatgacga cggggccaat tggtgccttg ctactcaggc
     5461 ttacggtgcg caaggcaacc acggcacgcc gggggttacc aatgactcct gcgcctgcaa
     5521 gccgctcacc gcatgcccca cgggcttgaa ttgcggcgat taccccgatg gttgcggcgg
     5581 caccctcagt tgcggaacct gcagcggttc cgagacgtgt ggcggcggtg gcacgcccaa
     5641 cgtatgcgga caagggcctt gcgtgccgac cacctgcgtg gccgaaggcg cggaatgtgg
     5701 ttctatttcc gatggttgcg gcaagaccct cgactgcggc gcctgcgacg ctcccgagac
     5761 ctgcggcggc ggcggccaag acaacatttg tggttgtacg cctaccacct gcaccattct
     5821 tggtctcaac tgcgggcctg tgcccgacgg ttgtggtggc acgctcgact gcggcacctg
     5881 cgccgctccc ttgacctgcg gcggtggcgg tgaagacaat gtctgcggct gtacgccgct
     5941 caccgcatgc cccgcgggct tgaattgtgg aacaatttcc aatggttgcg gcggcaccat
     6001 cagttgcggc acctgcacca ctcccgagac ctgcggcggc ggcggagttc ccaacgtctg
     6061 cagccttccg catcccgaag ccgggcaagt catcttctcc gaaatcatgc ccaaccccaa
     6121 aaccctcgac gataaagacg gcgaatggtt tgaattgcac aatccaacca cctccgcttt
     6181 caacctgcgg gattgcacca tcaaggacaa cagcgcgaca ttcacgatcg gcgcggattt
     6241 gaatatccag gcaggcggct atctcgtgtt cgcgcgaagc agcagccctg gatttacgcc
     6301 ggatatggtg tttaccggcc tcgaactcgc caacaccgac gaccagctca cgctgacctg
     6361 cgcctccgtc atgatcgacg aagtcaagta caccagcgct ttcccgtttg gcgacggggt
     6421 tgcaatgaac ctcgatccca cgcacttcaa cgcgaccgat aacgaccttg cggccaactg
     6481 gtgcaaggct accgaaagct ataacggtga tcgcggtacg ccgggcaaag ccaatacatc
     6541 gtgcggctgc acgccgctca ccgcatgccc gtcgggcttg aattgtggcg attacaacga
     6601 tggttgcggc ggcaccatca gttgcggcac ctgcatcgct ccccagacct gcggcggtgg
     6661 cggaactcct aacgtctgcg gctgcacgcc gctcaccgca tgcccgtcgg gcttgaattg
     6721 cggcgattac aacgatggtt gcggcggcac catcagttgc ggcacctgca tcgctcccca
     6781 gacctgcggc ggtggcggaa ctcctaacgt ctgcggctgc acgccgctca ccgcatgccc
     6841 gtcgggcttg aattgcggcg attacaacga tggttgcggc ggcaccatca gttgcggcac
     6901 ctgcaccgct tcccagacct gcggcggtgg cggtgaggat aacgtttgcg gtctacctcc
     6961 acccgacgtg gggcaagtca tcttctccga aatcatggtg gaccccacca cggttggcga
     7021 caccgggggc gaatggttcg aactgcacaa cccaacgacg agcgctttcg acctcaaagg
     7081 atgcgtggtg cgagacaata gtggctcgtt caccatcaat acttctctta tcattccctc
     7141 ggggggctac cttgctcttg cgaacggtga ttcccctgga tttacgcccg cttacgttta
     7201 ttccgggatc gctctcgcca acggtggaga cgaactgcat ctcacttgcg ccagcaacct
     7261 catcgatgag gtgatcttca ccagcggctt cccgattcaa gctggaaaag ctctgaatct
     7321 atcgccagac agccttacgg ccacggccaa tgacgacccg gccaactggt gcatcgcaag
     7381 tgtgagttac aacggtgatc taggcacccc cggcgccgcc aacagttctt gcggttccgc
     7441 gcccacctat accatcggat ggtgcaatct gcagttccct cccaaaatgg tctccatcca
     7501 aggtgagaag cgcgacgtat acggccgcgt tttcatcgat ggactcacca accagggctc
     7561 cgggcccaat cccaactccc aggtgatcgg ccaggtgggc ttcggtagca atgactccga
     7621 tcccaccacc tggacgaact ggactgcggc cacggccaac gacggattct ccgatcccgc
     7681 caacgatgaa tatatggcga cggtgaccat ccccacggcc tcggcaaccc attacgatta
     7741 tgccttccgg ttctccggcg atggtggaaa cacgtggaaa tactgcgatc tcgacggatc
     7801 cgacaacggt tacgccccgg cccaagccgg tgatctgctg tccaagacgt cgcacagcat
     7861 cgattggtgc cgtctacaaa ccgcttcgat ctccggtgcg gaaggaacga acgccacggc
     7921 gtacggccaa ctctatatcg aaggattgac ggatcttaac aagacgggca acgaccctca
     7981 tccgatcgtc aaaggccagg ttggttatgg accggatgca tcgacgccac ccaattcatg
     8041 gacctggatc gatgctgatc cgaatcccgg atggacccca tccgatagca acaatgacga
     8101 atacatggct acgttcccga tcccggcggc gtcgggttcc ccctacgatc tcgccttccg
     8161 attctccggc gatgatggtg ccacgtggac ctattgcgat ttggatggct cgacgactgg
     8221 cgggtatacg acggaccagg ccggagagat tacggtaacc cccgtcgcgc cggccacgct
     8281 gtttttcagc gaatatattg agggatccag caataacaag gcggtggaga tctacaaccc
     8341 gacgggagca gacgttgata tttcgcaatg ctcggtgaga gtctattcga atgcagcctc
     8401 gtcacccagc aaaaccatca ctccaccggc aagcaccccc catctggcat cgggaggagt
     8461 ctttgtaatt tgccacaaca gcgcagtttc aggcataaaa gataaatgcg attatcagac
     8521 agcatccctt gacttcaacg gcgatgatgc ggtggagctt gtttgcaacg gcacgaccct
     8581 cgatgtcatt ggacaaatag gcttcgatcc cggtacggct tggggatcgg gtaatacatc
     8641 tacagtcgat cataccctcg tccgcaaatg cgggatccaa tccggtgata cggatggctc
     8701 ggatcctttc gatccagccg tggaatggga tggctattct caggacacct tcacctactt
     8761 gggctcccat acgctcacct gcccctgagt ttccgcttcc gtggggcggc atgaggccgt
     8821 ccctatctcc ccttcctcgc tcgatcgact tttgacatgg gcggtcatgg ccgccgccgt
     8881 cacccttttt cccaacctcc cttaccgcat tcaccgcggc gcgagcgatg gttacgatca
     8941 agtagacctc gcggtgtgaa atgcgcgtaa cctattgtaa tattagcata atttaggcaa
     9001 cgggacaggc gacggaggca ggtgggcttt cgttcaaaaa ggtcgcgaag gggagatgct
     9061 caaggcggcg tctctttttt ctttgtcgag ttttttgcgc aagtcctcgt tttggggatc
     9121 gagaacgagg gcctgctcgt acgcgcgagc cgcctgggca tgcaatccca gggcgctgga
     9181 agtatttccg agggcagtcc aggccgcgac gttgcggggg tcatgacggg tggcgcgttg
     9241 gaacgcgctg gtagccgcgc cgaggttttt tcgagcgagc tgggttctgc cgatccaaag
     9301 gtgggtttgg gcgcaagcgg ccggactcga gcatcctgag gcggcgacgg catcgagcag
     9361 agtctccaac tcgctttcgt gcccgagggc cacgacgatc ggcacgcgcg cgcgcaagca
     9421 ttcatttcgg tctttgacgt gatcacaagc ggcggccagg attttatctg ctttctcggg
     9481 ttggtggagc gccatgaggg catcggcgcg gaggcgggcg gcccttgaag tatccggctc
     9541 gcttttttcg atttgagcga tatgctcttc gatttgatcg gcgcaggccc gtcgcttggc
     9601 ttccccttca cacaaggggt tttcggcgtt tttcagggcg tggattcggt ttatgacctg
     9661 gcgaatccgt gggccgacgc gcgccgggtc ccgcttcagc aattcttcgt cgtatcgctg
     9721 gctggtttcg gggtcgcggg tgtgcatcca acccgccaag gtatccatga cgatcaggcc
     9781 atcgtcgccc tccgggatca tccgatcgag gtctttgcct aggactttgt aatggacggc
     9841 gagaccgaca gcggtggtcg tgagcgcggg ttcgtcgcga cacgcgaact tgagttccat
     9901 cagcgcttga tgacgcgcat gccgagcgaa aaggatttcg gcgagcagga ggtgggctct
     9961 gccatacacc ttggcgcggg tgaacacccg ttgcaggtag ggaatggggt tggcatcccg
    10021 ctcgcgccac gcgcgttcgg ccccgacgag gggaatatag ggttcggcgg gatgtcggag
    10081 catggcggcg cgaatcaacg gattgaagcg gtctttggac attcgcggcc cttcgatgag
    10141 ccgacgaaac tccaggcgat cgtcgagcgc ggtgcgcatg ccccgggtcg ctacgagcaa
    10201 aagggccagg cccccgagca cggcaaagcc cacggtcagt ccacgacgtc cgaagcggga
    10261 agcccaggga tggccgagag atcgagccac accccgtcgt cgcgtgtctc cccagcatgt
    10321 cccgatgagg gcgacggcgg agatggcgac accgggcatt tccaggctga aatcgaccca
    10381 gttttgcaaa acgaggatga agattcccgc gacggctccg gcagcgacgg cgcttcgcgt
    10441 ggcgcccatg cgggacgggc ggatcagcca ggcgaaaaag ccgacggtga g
//
LOCUS       DAOSOR010000150        10377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6435_length_10377_cov_4.289285, whole genome shotgun sequence.
ACCESSION   DAOSOR010000150 DAOSOR010000000
VERSION     DAOSOR010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 10377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10377
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6435_length_10377_cov_4.289285"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /gene="folK"
                     /locus_tag="PK710_10075"
     CDS             <1..513
                     /gene="folK"
                     /locus_tag="PK710_10075"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOT10107.1"
                     /translation="NLGHRLGSVLSGASMMARWAGAEEAILSPLYETVPVGGPPQGDF
                     INAALTFKTNLSAGTLLTMALAIEQRHGRVRVERFGPRTLDIDVLWIEGEIIDQAGLQ
                     VPHPRLLERAFALRPMLDLVPTAVDPATRLPYEASMALLSVCGLRKLDRSSRLRMHEL
                     SPSFEAAALP"
     gene            complement(548..1264)
                     /locus_tag="PK710_10080"
     CDS             complement(548..1264)
                     /locus_tag="PK710_10080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOT10108.1"
                     /translation="MPSRFGHDAPAPTLSLLTRSAYPFALPFSHTLAPHKFGWPLLVL
                     VSLSPACQPRTPQPPVTRVTFPSYPPPPPTAANSPQPSSNPLDSQSFPPYPPPPPQPF
                     EPKPKAPYSEGDALRQSFAGRPPKRSWSGEATYYHDSLTGNLTASGDQYDPTRYTAAH
                     RSLPFGTILRIVHPNSGRDVFVCVNDRGPFGRRSLLVDLSRAAAEELGLLRHGVMTVR
                     TDVMEYGTGRKGACNRGKNH"
     gene            1465..1965
                     /locus_tag="PK710_10085"
     CDS             1465..1965
                     /locus_tag="PK710_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HOT10109.1"
                     /translation="MHCTELFSSARASLMSAASGGAGVSVDFDGSVIIQFVVFLLLFI
                     VLKPILLDPFLKVVEAREKRTDGAKAEARQMDERAGEIIKRYESEMEKVRKVAGEERE
                     RLRSEAQKLEAQILGEARTEAARVTAEGKEKIRKEAESIRFELGQFSGELARETAAKV
                     LGREIS"
     gene            1965..2690
                     /locus_tag="PK710_10090"
     CDS             1965..2690
                     /locus_tag="PK710_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HOT10110.1"
                     /translation="MRLRWVLAVVSVSIALTSFAWADDSLQEGSAAQGREHAAHEEHG
                     HHAPGSINWFYGFLGTDAEAEPSLLWRKPDMPPPFMANLINFAVFAYIVVHFGRKPLQ
                     EALHSRKQALMKEIDAATKMREEASSRLEKYEDKLKRVDEDIERIRDDFRAQGQREKE
                     RILAEAKEARDRMLKDAQFMIEQESKQMRNVLMRDTVEAAMKAAEALLQSKMTAADHE
                     RLAEEYIRQLSSSGLTITKGGSA"
     gene            2687..3229
                     /gene="atpH"
                     /locus_tag="PK710_10095"
     CDS             2687..3229
                     /gene="atpH"
                     /locus_tag="PK710_10095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOT10111.1"
                     /translation="MSYEAIAGRYAQALFDIGIETGTLGKLADEISAFADTWTASDEL
                     RAVLDNPLMSETDREALLDAIAKRLGSGEITKNTLRLLARRRRLPVLPSIARALRSMS
                     DDKEGVVRATVVSAKPLADSYAQRLQAALQSLTGKKVLLTMQVEASLISGVVTRMGDT
                     VIDGSLRTRLDGLRQQLLAE"
     gene            3290..5053
                     /gene="atpA"
                     /locus_tag="PK710_10100"
     CDS             3290..5053
                     /gene="atpA"
                     /locus_tag="PK710_10100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOT10112.1"
                     /translation="MMQLRAEEISQIIRKQIQSFDKAALVTETGTVLTVGDGIARVYG
                     LEGAMASELVEFPGGLFGMVLNLEQDNVGVAIFGEATHVKEGDTVKRTGRIADVPVGE
                     ALVGRVVNSLGEPIDGKGPLDTKLRRRIEIKAPGIIERQSVHEPLQTGLKAIDSMIPI
                     GRGQRELIIGDRQTGKTAVAVDTIINQKGQNVYCFYIAIGQKQSTVAQVVDRLTSFGA
                     MEYTTVVSAGASELAPLQFIAPYTGCTMAEYFRDTGRHALCIYDDLSKQAVAYRQLSL
                     LLRRPPGREAYPGDVFYLHSRLLERAAKLSQRWFVMKKGADLPAGNRDFRGEDGKVHV
                     GPEAKKFAMHAAYKLVDTEAFDSFDKADKADKKKLEEQLSKKLEESNYEVKMDPHSGG
                     SLTALPVIETQAGDVSAYIPTNVISITDGQMFLETDLFYSGQRPALNVGISVSRVGGN
                     AQIKAMKQIAGSLRLDLAQYREQAAFAQFASDLDAATRRQLERGARLTEILKQGQYAP
                     LRVSKQIAIIYAGTNGYLDEFPVSSLGRYETEMYDYMESQDPKIWEDIETKKELDGDM
                     KKRLNALLEGFNKKFVADKKN"
     gene            5104..6072
                     /gene="atpG"
                     /locus_tag="PK710_10105"
     CDS             5104..6072
                     /gene="atpG"
                     /locus_tag="PK710_10105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOT10113.1"
                     /translation="MASLKAIRTRIASVKSTQKITRAMKMVAGARLNRAQSRILALRP
                     YAIKTHTVLANCAAMSASQKANATTGENTENPPDSPDLTASADLDSELAHPLLATRPE
                     KNCLFLVLTSDRGLCGAFNSSINRTMEREWHRRENLGQNVQLGLVGRKGRDYFKRRNA
                     PIFKVFQNVWDDLSLDKARLVCQSLIEPFLEGKVDSIYLVYNEFKSAMTQRVVIEPLL
                     PISQERLEDDEEVVTRDLDDFIFEPDRTGLLDRLVPMYVEVSIYRALLESNASEHGAR
                     MTAMDAATKNAKEMIAALTLQYNRARQAAITTELGEIIGGAEALKG"
     gene            6367..>10377
                     /locus_tag="PK710_10110"
     CDS             6367..>10377
                     /locus_tag="PK710_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10114.1"
                     /translation="MRAFVRRWLLQTNRVLIVGALLITFISSFVIGLAFHLPLPPSRR
                     LATRIVMKQVSRSIRGSIEIDQISRLHLGGFEALDVTIRDPEGEVVLFAPRLRARANL
                     TTIALSFMPWSDRAQVPISWVRAEQIDVTLRKDTDGTISLGTTFLAPLRAVPPTPAPT
                     PSAAARPLIVDLQSIEIGTIVANGNVGIPVDWRTRTVHGSVRIAPEGVQIDTRAFPIA
                     FEKVLQEPIVATVDYHFRSPSLMWADVMATYRDLSSSGRVQLKGTEMLIDLSIPQTDP
                     RALAPFVPESVELADAFEGRIEAHGPIPVLDTHVRLMSERMLLDATGSIRLAPTFETD
                     LDVDLRRIDLQRLVKSAPRSQLDADIRVRVSLDEEGRPRARLSGDTSPTILEGTLIPG
                     MDVDVLVDAKGASGSAHIHEMGMPVRADFFFSPTHGLRLSAAANVPSLQAVPRFKRAV
                     QGYAVAKVEGTYHQGIVDAKVAVSGGNLSREGVRIGSASVELDLVGPPDHLRMNASMS
                     GRGAQFGALHWDAFHSVVQGPMASPHVRVHLQDRMLPNVTATASVDMRGQLAAKNVDI
                     KLEQGREIIEVKTPAIEIRSNIIDLGEFSIEGLGSTLEGVAALGPKGFDVQASSDSID
                     MDRALSFFPLLGPRITGVVGVDIDLRGVGSESYGCIRLDVADAEVKTLVPIPRVDGSI
                     RAQFTGRLVDVYADMALGATGDSYEAGLDDPGVCIPTQPRGRDGLVTARATGSLTLTG
                     APMDVSSWKEATGTGRLEQLAIDLDRVSEMYGGVLSLVNDEVGGSIPIVKGKVSLQAD
                     VVLERADQLPSWSLTASSRGLQVSMPESTDRVAGDIVASASMSSAGMLLGSVCLRKGA
                     ASSLGESCNPNDRDLLASVGWLAALDYRRLVDDPESWKQVLLEAQVQGNAVVHDRSLV
                     DLFSPWKRESRWPLEAGYASANIGFRGTALEPHIDYRMNVTRIASSDPGWRAPVSVCV
                     RGQYDGDKAWAHVDLRRSADNQSANAVCALSPTIPVHGLGSVDATVQVAWKDVLAWSS
                     LPRIPWVANMDAAIAGFDLADIPVLADQGYSGQVRMTAAIRGIGAKPTFDVQVGLEAL
                     RMGSTVSYDQSHLWLQAGDSGFHGALAFIDVDDNGEPTSSLRVSMDTDQVQWMNGWML
                     TRYGGKPVRIAVDANRFKLGMLSPLAQPVFSYVDGELTGKAQATWSPTEGKSRVESLS
                     FYVDNGAFQVPIIGQEFLQVKGLLQAADSERIFIEHVQAQSLTGGIEARATVDLDEFD
                     FRHIDASIWTKQGEKIRLTFAGMPVGDMYGTINVAIDPKNEKRNTKENKVVIEFDRVT
                     VDLPRTDLRSVQQLEDN"
BASE COUNT         2213 a         2600 c         3296 g         2268 t
ORIGIN      
        1 aatcttggcc atcgtctggg aagcgtgctt tccggagcgt ccatgatggc ccgttgggcg
       61 ggggccgagg aagccattct ctcgcctttg tatgagaccg tgccggtggg aggaccaccg
      121 caaggcgact tcatcaacgc tgcgctcacc ttcaaaacca acttgtcggc aggtaccctg
      181 cttacgatgg ccttggcgat cgagcagcgg catggtcgcg ttcgtgtcga acgcttcggt
      241 cctcgtacgt tggacatcga tgtgctttgg attgaaggtg aaatcatcga tcaggctggg
      301 ttgcaagtgc cgcatccgcg attgctggag cgagcttttg cgttgcgtcc catgctcgac
      361 ctggtgccga ctgccgtgga tccggcaaca cgcttgccgt acgaggcctc gatggcgtta
      421 ttgtctgttt gcggtttacg aaagttggat cgttcctcga ggcttcggat gcacgagttg
      481 agtccttcct tcgaggcagc ggcacttccc tgagccattg gcaggggccg acgatgggct
      541 gtggtgttca gtgattcttg ccgcggttgc atgcaccctt ccggccggtc ccgtattcca
      601 tgacgtcggt acgaacggtc atgacgccgt gacgcaaaag tccgagttct tcggcggcgg
      661 cgcgggaaag gtctacgagc agcgatcgac ggccaaaggg gcctcggtca ttgacgcaaa
      721 cgaaaacgtc gcggccggag tttggatgaa cgatgcgtag gatggtgccg aagggcaggg
      781 agcggtgtgc cgcggtatag cgagtggggt cgtattggtc gcccgaagcg gtgaggttgc
      841 cggtgagcga gtcgtgataa taggtggcct cgccggacca ggaacgtttt gggggacggc
      901 cggcgaacga ttgacgaagg gcgtcgcctt cggaatacgg ggctttgggt ttgggttcga
      961 agggttgggg aggcggagga gggtagggag gaaatgactg ggaatccaat gggttggaag
     1021 atggctgggg ggaattggct gcggtggggg gtggtggtgg ataggaggga aacgtgacgc
     1081 gagtgacggg aggttggggg gttcgaggct gacatgctgg cgatagcgag acgagcacca
     1141 gcaggggcca gccgaatttg tgtggagcaa gggtgtggga gaagggcaag gcgaaaggat
     1201 acgcgctgcg tgtgagaagg gaaagggtcg gagcaggcgc gtcatgaccg aatcgtgagg
     1261 gcacgttctc gggaaaggct cacggaataa gtgtaactaa ttgaaaatac tagataattt
     1321 tttagtcctt tcggcttgcc acccgtccct agacacgtca atcgagaagt gttagacgcc
     1381 ccgcggcatc ttttcgggac gtattatccc gagaatgtgg cctaacgttc cggtgttttt
     1441 ttgatccggc tcatcgttca caccatgcac tgcacagaac tattttccag tgcccgtgct
     1501 tcgctgatga gtgccgcctc tggcggtgct ggggtcagcg tcgacttcga cggctccgta
     1561 atcattcaat tcgttgtatt tttgctgcta ttcatcgtgc tcaagccgat tcttttggat
     1621 ccttttctaa aagtggtcga ggcgcgggag aagcggaccg acggtgcgaa ggccgaggcg
     1681 cgtcagatgg acgaaagggc tggggagatc atcaagcgtt atgaaagtga gatggagaag
     1741 gtgcgcaagg tggcgggcga ggagcgggag cgtcttcggt ccgaggcgca gaaactcgaa
     1801 gcgcaaattc ttggagaagc gcgcacggag gcagcacggg tgacggcgga aggcaaggaa
     1861 aaaatccgca aggaagcgga atcgatccga ttcgagcttg ggcagttttc gggggagctg
     1921 gcgcgagaga cggctgccaa ggtgcttgga agggagattt cgtgatgcga cttcggtggg
     1981 tattggcggt tgtgtccgtg tcgatcgcgt tgacttcttt cgcatgggcg gatgattcgt
     2041 tgcaggaggg gtcggccgcg caggggcggg agcatgctgc gcacgaggag catggtcatc
     2101 acgcgcctgg gtcgatcaac tggttttacg gttttttggg gacggatgcg gaagcggagc
     2161 cgagtttgct ttggcgaaag cctgacatgc cgcctccgtt catggcgaat ctgatcaact
     2221 tcgcggtgtt tgcgtatatc gtggttcatt tcggccgaaa gcctttgcag gaggcgctcc
     2281 acagccgcaa gcaggcgctc atgaaagaga tcgacgcggc cacgaaaatg cgtgaggagg
     2341 cttcgagtcg actcgaaaag tacgaggata agctcaagcg tgtcgatgag gacatcgagc
     2401 ggatacgcga tgattttcgc gcgcaggggc aacgggagaa agagcgcatc ttggcggagg
     2461 ccaaagaggc ccgggacagg atgctcaaag atgcgcaatt catgattgag caagaatcca
     2521 agcagatgcg caacgtattg atgcgcgata ccgtggaggc cgcgatgaaa gccgcggagg
     2581 cattgctcca atccaagatg acggccgcgg accacgagcg actcgcggaa gagtatattc
     2641 gtcaactatc ctcatccgga ttgacgatta cgaaaggggg ctcggcgtga gttacgaggc
     2701 cattgcgggt cgctacgcgc aagctctgtt cgacattggc atcgaaacag ggaccctggg
     2761 aaaactcgcc gacgaaatat cagcgtttgc ggatacatgg accgccagcg atgaacttcg
     2821 cgccgtactt gataaccctt tgatgagcga aacggaccga gaagccttgc tagacgccat
     2881 agcgaaacgt cttggttccg gagaaatcac gaaaaatacc ctacgccttc tcgcgcgtcg
     2941 acgaagactg cccgtgttac cgagcattgc gcgggctttg cgctcgatga gtgatgacaa
     3001 ggagggggtg gtgagggcga ccgtggtgag tgccaagccg ctcgccgact cgtatgcgca
     3061 gcgcctgcag gccgcgcttc aatcgctcac gggaaaaaag gttttgctga ctatgcaggt
     3121 ggaggcttcc ttgatatccg gtgtggtgac gaggatgggc gatacggtca tcgacggttc
     3181 tcttcgaacg cgtctcgatg ggctccgcca acagctcctt gcggagtgac ggataagcaa
     3241 cagcgatggg aatgactagt gccaaagcag gcacgttgtg tacgagggaa tgatgcagct
     3301 ccgagccgaa gaaatctcgc aaatcatccg aaagcagatc cagagcttcg acaaggccgc
     3361 cctggttacc gaaacgggta ccgtgttgac ggtcggcgac ggtatcgcgc gtgtgtatgg
     3421 cttggaagga gccatggcga gcgagttggt cgagttcccg ggtggcctgt tcggcatggt
     3481 gctcaacctg gaacaggaca acgtgggtgt ggccatcttc ggcgaagcca cgcacgtcaa
     3541 ggaaggcgat acggtcaaac gcaccgggcg aatcgccgac gtccctgtcg gggaagcttt
     3601 ggtcgggcgt gtggtcaact ccctgggcga gccgatcgac ggcaaagggc cgctcgatac
     3661 gaaactgcgt cgtcgcatcg aaatcaaagc ccctgggatc atcgagcgtc agagcgtgca
     3721 tgagccgttg cagactggtc tcaaggcgat cgattccatg atccccatcg gaagaggtca
     3781 gcgcgaactc atcattggtg accgtcaaac cggcaagacc gcggtcgcgg tagacacgat
     3841 catcaatcaa aaaggccaaa acgtatactg tttctacatc gcgatcgggc aaaaacagtc
     3901 gacggtcgcg caagtggtgg accgtctcac gagctttggc gccatggaat acacgactgt
     3961 ggtgtcggcg ggcgccagtg aattggctcc tcttcaattc attgcgccct acacgggctg
     4021 caccatggcc gaatacttcc gcgatacggg tcgtcacgcc ctgtgcattt acgacgacct
     4081 atccaaacaa gctgtcgcct accgccagtt gtctttgttg cttcgtcgtc ctccggggcg
     4141 tgaggcttat ccgggagacg ttttctatct tcactcgcgg cttctcgaac gggctgccaa
     4201 gctatcccag cggtggttcg tgatgaaaaa gggagcggat ttgcccgctg gcaatcggga
     4261 tttccgggga gaagatggca aggtccacgt aggtcccgag gctaaaaaat tcgccatgca
     4321 tgcggcatac aagcttgtcg ataccgaggc tttcgactcg tttgacaagg cagacaaggc
     4381 agacaaaaag aaactcgaag agcaactcag caagaaactc gaagagagca actatgaggt
     4441 gaaaatggat ccgcattcgg gcggatctct tacggctctt ccggtgatcg aaacccaagc
     4501 tggtgacgtc tccgcgtaca ttcctaccaa cgtgatttcg atcacggacg gacagatgtt
     4561 tttggagacg gacttgtttt actcggggca gcgtccggcg ctgaacgtgg gcatttccgt
     4621 gagtcgtgtc ggtggtaacg cgcaaatcaa ggcgatgaag caaattgccg gttcgcttcg
     4681 tttggacctt gcgcagtacc gggagcaagc ggctttcgct caattcgctt ccgacctgga
     4741 tgcggccacc cgtcgtcagc tcgagcgtgg cgcgcgcctt acggagatcc tcaaacaagg
     4801 ccagtatgcg ccgcttcgtg tctccaagca gattgccatc atctacgcgg ggacgaacgg
     4861 gtatctcgac gagttcccag tcagttcttt gggacgttat gaaaccgaga tgtatgacta
     4921 catggagtcg caggacccga agatttggga agacatcgag acgaaaaaag aactcgatgg
     4981 cgatatgaaa aagcggctca acgcgctttt ggaaggcttc aacaaaaaat tcgtggccga
     5041 taagaaaaac tgagttgaac cctcttccgt gtcgcgggac ctttttgtgt tgagaggact
     5101 gccatggcgt cactcaaagc catccgaacg agaatcgcga gtgtcaagtc gacacaaaaa
     5161 atcacgcggg ccatgaaaat ggtcgctgga gcgcggctca atcgtgcgca atctcgcatc
     5221 cttgccctgc gcccctacgc gatcaaaacc catacggtgc ttgccaattg tgccgcgatg
     5281 agcgcttctc aaaaagccaa cgccacgaca ggcgaaaaca ccgaaaaccc gccggattct
     5341 cccgatctca ccgcctctgc cgatctggac agcgaactcg ctcacccctt gctcgctacc
     5401 cgtcctgaaa aaaattgctt gtttctcgtg ctcaccagcg atcggggact atgcggagcg
     5461 ttcaactcaa gcatcaaccg caccatggaa cgagaatggc accgtcgaga aaatttggga
     5521 caaaatgtcc aactcggact cgtgggtcga aaaggccgcg actacttcaa gcgtcgtaac
     5581 gccccgatct tcaaggtgtt ccaaaatgtc tgggatgatt tgtccctcga taaggctcgc
     5641 ctcgtctgtc aatcgttgat cgagcctttt ttggaaggaa aggtcgactc gatttacctc
     5701 gtctataatg aattcaagag cgcgatgacc caacgggtcg tgattgagcc gttgcttccc
     5761 atttcacaag agcggctcga ggacgacgaa gaagtcgtga cgcgcgatct ggacgacttc
     5821 attttcgagc ctgatcgaac cggacttctt gatcgtctcg tcccgatgta tgtggaagta
     5881 tcgatctatc gcgcgttgtt ggagtcgaac gcaagcgagc atggggctcg aatgaccgcc
     5941 atggatgcgg ctacgaaaaa cgcgaaagag atgattgcgg cattgaccct tcaatacaac
     6001 cgagcgcgtc aggccgccat caccaccgaa ctcggggaga tcatcggcgg cgccgaggcc
     6061 ttgaaaggtt gatgaaacga gtccgcggat acaaccccgc tcatggcgct cccgtttcat
     6121 tgtaggcttg tgcagggatg gccgtagacg cctgccccaa gtaaacacag gctcctgaaa
     6181 atacgtttca accgcacgat cttggctcga gacaaagcat tccgtcacgt gctttcggtt
     6241 ccatgcgtga agcctgctcg agggtgttgt aggctttcgg agcgtgcctt tcctcctttt
     6301 accctgcctg gcttctacca cacatccgcg gtggcaaagc cgaggtgtcg catgggggaa
     6361 gaccgcatga gagctttcgt gcgtcgatgg ctgctgcaaa ccaaccgtgt gctcatcgtt
     6421 ggcgcgcttc tcatcacgtt catctcgagt ttcgtcattg gactggcctt tcatctgcct
     6481 ctgcctcctt cccggcgatt ggccactcgt atcgtcatga agcaggtgag ccggtccatc
     6541 cgcggttcca tcgaaatcga tcaaatctct cgattacatc tgggaggttt cgaggctctc
     6601 gacgtcacca ttcgagatcc ggagggggag gttgtgcttt ttgcacctcg gttgcgagcg
     6661 cgtgccaatc tcaccaccat tgctctatcg ttcatgcctt ggagtgatcg cgctcaggtg
     6721 cccatttcgt gggttcgggc cgagcaaatc gatgtaacgc tgcgcaagga cactgatggc
     6781 accatctctt tgggcacgac ttttcttgct cccttgcgtg ctgttccgcc aactcctgcc
     6841 ccgacgccat ccgccgccgc tcgtcccctc atcgtcgacc tacaaagcat cgagatcgga
     6901 acgatcgttg ccaatggcaa cgtgggtatc ccggtggatt ggaggacgcg aacggttcac
     6961 ggttcggttc gaattgctcc cgaaggcgtg caaatcgata ccagggcttt ccccatcgcg
     7021 ttcgagaaag ttttgcaaga gcccatcgtg gcgacggtgg attatcattt tcgctctccg
     7081 tcgctgatgt gggcggatgt gatggccacg tacagggact tatcgtcttc ggggcgggtg
     7141 cagctaaaag gaactgagat gctcattgat ttgagtattc cgcagacaga tccgcgagcg
     7201 ttggcaccct ttgttccaga aagcgtggaa cttgcggatg ctttcgaagg ccggatcgaa
     7261 gcgcacggtc ctattcctgt tctcgatacg catgtgcggt tgatgtccga gcgaatgttg
     7321 ttggatgcga caggaagcat ccggctggct ccaacgttcg aaacggactt ggacgttgat
     7381 ttgcgacgga tcgacttgca acgtttggtg aaaagcgccc ctcgttccca actggatgcg
     7441 gatattcgtg ttcgtgtatc cctcgatgag gagggacgac cgcgggcgcg attgtcggga
     7501 gatacgtcgc ccacgattct tgaaggaacc ttgattcccg gcatggatgt ggatgtgttg
     7561 gtggatgcca agggggcgag cggttctgct cacatccatg agatgggcat gccggtgcga
     7621 gcggactttt ttttctctcc cacccatgga ctgcggttga gcgcggcagc gaacgttccg
     7681 tcgttgcaag ccgtgccaag gttcaaacgt gcggtacaag ggtatgcagt agcgaaagtt
     7741 gaaggtacgt accatcaggg catcgtggat gcgaaagtgg cggtgagtgg cggtaattta
     7801 tcgcgcgaag gcgtacgaat cggctcggcg agtgtcgaac tcgatcttgt gggaccaccc
     7861 gatcacctgc gaatgaatgc gtcgatgagc gggcgtgggg ctcaattcgg agcgcttcat
     7921 tgggacgcct tccattcggt cgtgcaagga cccatggctt cgcctcacgt gagagtccac
     7981 ttgcaggatc gaatgctgcc gaacgtgacg gccacggcca gtgtcgatat gcgcgggcaa
     8041 cttgcggcga aaaacgtcga catcaagctc gagcaggggc gggagatcat cgaagtcaag
     8101 accccggcga tagaaattag aagtaatatc atagatttag gagagttttc catcgaggga
     8161 cttggctcga ccctcgaggg ggttgccgcg cttggaccga agggattcga cgtgcaagcg
     8221 tcatcggaca gcattgatat ggatcgggcg ctgagctttt ttccgctcct tggcccacgg
     8281 ataacgggag tcgttggggt cgatatcgat ttgcgagggg tgggaagcga gtcctatggg
     8341 tgtattcgac ttgacgtagc ggatgcagaa gtcaaaacgc ttgttcccat ccctcgggtg
     8401 gatggttcga ttcgtgctca atttacaggt cgtcttgtgg acgtatacgc ggacatggcg
     8461 ttgggtgcga cgggtgattc ttacgaggcg gggttggatg atccgggggt gtgtattccg
     8521 acacaaccaa ggggtcgaga tgggttggtt accgcgcgag ccacgggttc cctgacgctc
     8581 accggggcac cgatggacgt gtcgtcatgg aaggaggcga caggcacggg gcgcttggag
     8641 cagttggcca tcgatttgga ccgtgtttct gaaatgtatg ggggcgtatt gagtttggtg
     8701 aatgacgaag tgggcggttc gataccgatc gtgaagggaa aggtatcgtt gcaagcagat
     8761 gtggtgctcg agcgtgcgga ccaattgccg agttggtcat tgactgcttc ttcgcgtggt
     8821 cttcaggtat cgatgccgga atcgacggac agagtggcgg gggacattgt tgcttccgcg
     8881 tcgatgagca gtgcgggcat gctgcttggt tcggtgtgtt tgcgcaaagg cgccgcgtcc
     8941 tcgctcggag aatcctgcaa ccccaatgat cgcgatctat tggcttctgt tgggtggctc
     9001 gccgcgttgg attatcgacg gctcgtcgat gatccggagt catggaagca agtgttgctc
     9061 gaagcgcaag tgcaaggaaa cgcggtggtg catgaccgct cgctggtgga tctgttttca
     9121 ccgtggaagc gcgagagtcg gtggcctttg gaggcggggt atgcgagtgc gaacatcggt
     9181 tttcgcggca cggcattgga gccacacatc gactaccgga tgaacgtgac gcgcatcgcg
     9241 tccagtgatc cgggttggcg cgcgccggtt tccgtttgtg tcagagggca atacgacggg
     9301 gataaggcgt gggcgcatgt cgatctgcgt cggtcggcgg acaaccagtc tgcgaatgcg
     9361 gtttgtgcgt tgtcgccgac gataccagtt catggcttgg gcagcgtgga tgcaacggtc
     9421 caggtagcat ggaaggacgt gctcgcttgg tcgtcgctgc cgcgcatacc gtgggtagcg
     9481 aatatggatg cggccattgc tggtttcgac cttgcggaca taccggtatt ggcagaccag
     9541 gggtattctg ggcaggttcg gatgacggcg gcgattcggg gaatcggggc caagccgacg
     9601 ttcgatgtgc aggtggggtt ggaagcgctg cgcatgggtt cgacggtgag ctatgatcaa
     9661 agccatttat ggcttcaggc gggtgattcc ggttttcacg gggcgcttgc tttcatcgat
     9721 gtcgacgaca acggcgagcc tacatcgtcg ctacgtgttt cgatggacac cgatcaagtg
     9781 cagtggatga acggatggat gttgacccgt tatgggggga agccggttcg tattgcagtg
     9841 gacgccaacc gtttcaaact gggcatgctc agtcctttgg cgcagcctgt ttttagttat
     9901 gtggatggtg agcttacggg aaaagctcag gcaacgtgga gtcccacgga agggaaatcc
     9961 cgtgtcgaat ccttgtcttt ttacgtggat aacggagcat tccaagttcc catcatcggg
    10021 caagagtttc ttcaagtgaa aggattgttg caagccgcgg attcagaacg gatcttcatc
    10081 gagcacgttc aagcgcaatc cttgacgggg ggaattgaag caagagctac cgtcgacctg
    10141 gatgaattcg atttccgtca tatcgacgct tcgatttgga ccaaacaagg ggagaaaatt
    10201 cgcttgacgt tcgcgggtat gcccgtcgga gacatgtacg gcacgatcaa cgttgcgatc
    10261 gatccaaaaa acgagaagag aaacacaaaa gaaaataagg ttgtcatcga attcgatcga
    10321 gtcaccgtcg acttgccccg caccgatttg cgcagtgtgc aacagctcga ggacaac
//
LOCUS       DAOSOR010000151        10348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6453_length_10348_cov_3.885553, whole genome shotgun sequence.
ACCESSION   DAOSOR010000151 DAOSOR010000000
VERSION     DAOSOR010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 10348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10348
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6453_length_10348_cov_3.885553"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            345..2036
                     /gene="recN"
                     /locus_tag="PK710_10115"
     CDS             345..2036
                     /gene="recN"
                     /locus_tag="PK710_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOT10115.1"
                     /translation="MLLELRVRNFLLIDSLDLSLGPGFNVMTGETGAGKSVIVGALQL
                     VLGGRAGPHQIRPGAESAEIEALFELEPTDPGLETLRAGGLDNDHELVVRRVLLPSSR
                     SRAYLNGRLCAVAELSQLAADLADIASQHESVSLTNVASHLGYLDAFGGLDGEREQIA
                     RRVTELTALRAEVARLQTLAQQRAERQEFLLFQLNAIQEVDPRPHEREQLQNERSRLR
                     GATRLAEIAWNSAELLTGDQGVCDRLRGIVGDLATVADIDASLAEYGSDIQIALDRLG
                     ECARGLKKIAETTHADPARLDEVEHRLYRLEGLLRRHGPTESDAMAAMERLRAELVSL
                     EEVEESLRAVEKRFDQELSQAEQAAFSLSRKRTETASRLALGISEQLASLGMGGAQVR
                     VEVARHAGEGDLMVKGARLTAEGVDRVEFLIAPNKGIEPRPLRKIASGGELSRALLAI
                     KRVLASQGPAGLYIFDEVDAGVGGAVAERIGQALADIAKHHQVLCITHLAPIAALADY
                     HFVVRKSQDGHVARTQVVPVTGEDRVREIARMLSGKNVTKASMQAATEMMQQRQR"
     gene            2131..2961
                     /locus_tag="PK710_10120"
     CDS             2131..2961
                     /locus_tag="PK710_10120"
                     /inference="COORDINATES: protein motif:HMM:NF024246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT10116.1"
                     /translation="MTKRILRDIIEIDDDLCDGCGLCIPSCEEGALVLVDGKVRLAEE
                     ARCDGAGACLGHCPRGALRLIQREAERFDERYASGMQPSATQSAPSLESLVAFGKTAI
                     PQRPAAISAGCPGSRSMAWNTTPSSVSVPAKAASALTQWPIQLHLLSPGSSFLRAREL
                     LLAADCVAFSLGSFHGDLLEGRSLAIACPKLDDPSGYEEKIAEILAHCDVPALRVVIM
                     EVPCCGGLLRLASTAAKRAGYQGEIEVIVVSRFGEILQRKSLTPTPSPNESTGCACGG
                     "
     gene            complement(2988..3626)
                     /gene="coaE"
                     /locus_tag="PK710_10125"
     CDS             complement(2988..3626)
                     /gene="coaE"
                     /locus_tag="PK710_10125"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236339.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOT10117.1"
                     /translation="MSFKLLGLTGGIASGKSAVAAILSERGVPILDADEIARAVVAPD
                     SEALGMIVEAFGTEVLDPSGQLDRRKMAAIVFADSHARKRLESITHPRIRQRTEQRAS
                     QLARQGHPLAVYEAALIVENRLADKYRPLIVTLVDPATQIARLMGRDGLSETQARSRI
                     DAQMPLHEKASHADYVIDTQGSLQDVRRQTETILRMVCDQLGVPRHRYGLLT"
     gene            complement(3623..5113)
                     /locus_tag="PK710_10130"
     CDS             complement(3623..5113)
                     /locus_tag="PK710_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOT10118.1"
                     /translation="MTEPCPPQDQPLTAVAPMVLVVDDEKNIRRTVEMVLTGEGYRVT
                     GAASAEEALGLLARPNSPVDLILLDVKLPGMSGLEMLERMRTEEGLPQLPVIVISGHA
                     TVHDAVTAIKLGASDFFEKPLNRERVLVSVRNVLKTARLVRTVEDMREQFEARYEMIG
                     TSPSMQKLYREIDRVAPTKASVLITGESGTGKELVSRALHRLSPRAESPFVKVNCAAI
                     PRELIESELFGHERGSFTGAQTRKRGFFEQAHGGTLFLDEIGDMDLTAQAKVLRALQS
                     GEISRVGSEHLIHVDVRVLAATNKDLSKEVADGRFREDLYFRLAVFPIRCPALRERIE
                     DIRLLSDSFVTAFCKENGLRPMSIDTPVYDALRRRKWPGNVRELKNVIERAAILSGDL
                     ITIADLPEDPHDSPFDDDLGENSASSSSYASPISPSMASKSEEGRLTLREFREQAERN
                     YIVETLRALDWNISRTAIALGVERTNLHKKIRAYEITRGESKRNEP"
     gene            complement(5110..5925)
                     /gene="bamD"
                     /locus_tag="PK710_10135"
     CDS             complement(5110..5925)
                     /gene="bamD"
                     /locus_tag="PK710_10135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOT10119.1"
                     /translation="MNILQRLLALLATVLLVTMATGCSKELPAARPGTLQYSEEARLF
                     YEKAMRSFYERDWEEAEKRFQKVRREYGQSRYARWAELRLADIKFERDQLPEAISAYK
                     TFVQTRRLDPGIAYAQYRVCQALFQQINDTIMLPPQEERDQGPTTEAYQELRRFKREY
                     PTSRWDEQVDYMLLDVTGRLVRHELYVARFYLREGNFPAAASRARYALQHYENSGLEP
                     EALVLLGETYLKMKQRPQAREAFHRVLTSYPDSPFVVPARSFLQEISSGEVKP"
     gene            6054..6692
                     /gene="yihA"
                     /locus_tag="PK710_10140"
     CDS             6054..6692
                     /gene="yihA"
                     /locus_tag="PK710_10140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOT10120.1"
                     /translation="MTSRSHPIILEAQFSYGFTDLGSAPPSTWPEIAFAGRSNVGKSS
                     LLNALLQRKNLVRTSSTPGCTRQINAFLVRLEHLGPLHLIDLPGFGFAKRSKTERKQW
                     GSMIDQYLRDRAALAGVVVLVDVRRGPESLEASLIQYLQEIREPAIPYLFVATKVDKL
                     PAAQLKPRLDALRKTTPGRIIGFSAKTGLGREELWKQLLEAIPASNRQKACL"
     gene            6754..9129
                     /locus_tag="PK710_10145"
     CDS             6754..9129
                     /locus_tag="PK710_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10121.1"
                     /translation="MATIQLDQEILDVERIIDEKNHDEAFRRLILLRDRFGRRPEYRY
                     LKILFDATFAVRTDAELLSEVRTLVAEQQDFLEAVALLALLLDRTGDHERAIVFAREA
                     LRSPNSRAQMRARAILGEQAEQEVGGPPPSVNRFAASTAREAPTKPHVRPVNVPAADG
                     QSQTRSTKALFAVADDKQSSPPVDKAEAKEADLTLEGVAPPSNLDAAFAAVRPREPNS
                     SNLRLPSSPPIQDDRVSTLEGLPRPPLVPSKTFSGAQQPPDSLPSTVDFRISPSLPEQ
                     EPSQNSVGAEFIPFSPDQAPMPPPPTIPPGVVTANERSRPPTPHPSAPHAHRSSFSQA
                     STPPPRRSAFPPSPEPRADIAPETTASRLPSTPPTRRQPTPLPPEYAVDDTGVGREVE
                     RRRSRPSRQALSVPQPPEHIKGWFQFARQNQLQVVEGFSTAQMLLDLCERVVEGATSL
                     SSRPVPLDRRGLILVEEQLEALRGSRTKTQPVAERGAVTAAASFLLGLLLKECDGRAA
                     DTSVEDGACKVTLPSGATVRPLLVAAAFMRSRGPSLIETFDRAATSHMRRNPARVTTG
                     NAARVITGNAARVITGNAARVITGNSISQPPLRNARTPAIAAASSFTVLRRDLDASTL
                     GITDPAAPPAPQPPIDMRKVASEFWTSDLGREIIGSSRRVGTFTIADVDAIERHASKA
                     FNAVGFSPPGSPWPWMPSEEQDDLILAWGAILGEVLVSLYLGRWEADPGNPDDRFLFR
                     VILSSGVVAWPVAKAYMRLARGIPHDLTVYIDAVGRVVGRQALGARSWQGL"
     gene            9305..9811
                     /locus_tag="PK710_10150"
     CDS             9305..9811
                     /locus_tag="PK710_10150"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOT10122.1"
                     /translation="MTARCSSRYRLFVAKDEHKFSCAHMTLFPDGTKERLHGHNYRVG
                     AALEMQEGFLDFARVKSALATLCEELREHLLMPSQSAAVRVLSQDEISTEFSVCGKRY
                     VIPSDELLWLPMQNVVVEGLAEYLWGRIAQHLRAEFEQAGVGWLEVTVTEAPGQGARF
                     RADPRKGG"
     gene            9815..10183
                     /locus_tag="PK710_10155"
     CDS             9815..10183
                     /locus_tag="PK710_10155"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOT10123.1"
                     /translation="MALDKVMVRAIRVDASIGTLSWEKHELQPVQVDVDAVVDTEALL
                     GSGDLTKGVDFSIVIQTVRDVVTQGHVELVEVLADRIASALLAATPARSVRVEVRKYK
                     ACAEYASHVGVEVHRERTHG"
BASE COUNT         2049 a         3050 c         3008 g         2241 t
ORIGIN      
        1 ccctttcccg tgttttttcg ggctttgcta gccgccatga gtgttgccgt accctctgtt
       61 cgtggaagat taagacgttg cgttccgtac tgttgcagcc tcgggtctgc gaatcgactg
      121 aaaccctagt tcgcggaata tgcctccgtc aagcagccta gtgggattgt caaccctcgt
      181 tgtcgacaaa attgcgtttt ccaaaagaac cgcgaaatgc agctcggttg cggtgtcgtt
      241 gtccgaaaaa acacgacgaa acgcacgcac cttgccgggg gccgttttcg aatcgcgcgt
      301 ggaccttgac ggcttgccgg cttcgaacga caaggcgcct gatcatgctt ctcgagctgc
      361 gcgttcgaaa cttccttctc atcgactcgc tcgatctcag cctcggcccc ggcttcaacg
      421 tgatgaccgg agagacaggg gcaggaaaat ccgtcatcgt cggcgctctt caactcgttc
      481 ttggaggccg tgcaggcccc catcagattc gccccggcgc ggagtcagcc gaaattgaag
      541 cccttttcga actcgagccc accgaccccg ggctcgaaac cctacgcgct ggcggcctcg
      601 acaacgacca tgaactcgtc gtgcgaagag tcctcctgcc gtcttctcgg tctcgcgctt
      661 acctcaacgg ccgtctttgt gccgtggccg agttgagcca actcgctgcc gatcttgccg
      721 atatcgcttc ccagcacgaa agcgtctccc tgaccaatgt cgcgtcgcac ttgggctatt
      781 tggatgcatt cggagggttg gatggcgaac gagaacaaat cgcgcgtcgc gtcaccgagc
      841 tgaccgcgtt gcgagccgag gtcgcacgac tccaaaccct cgcccaacaa cgcgccgaac
      901 gacaagaatt cctgctgttt cagctcaatg ccatccagga agtcgacccc cgtccccacg
      961 aacgcgagca gctccaaaac gaaagaagtc ggttgcgcgg agcgacccgt ctagcggaaa
     1021 ttgcatggaa ttccgcagag ttactgactg gcgaccaagg cgtctgcgac cgtctccgtg
     1081 gtatcgtcgg tgacctcgcc accgtcgcgg atatcgacgc gtcgctggcg gaatacggat
     1141 ccgacattca aatcgcactc gatcgccttg gagaatgcgc cagaggattg aaaaaaattg
     1201 ccgaaacgac ccacgcggat cccgctcgcc tcgatgaagt cgaacaccgc ctctatcgac
     1261 tcgaagggtt gcttcgacgc catggtccca cggaatcgga tgccatggct gccatggagc
     1321 gattgcgtgc cgagctggtg tccttggagg aagtggaaga gtcgcttcga gcagtggaaa
     1381 aacgcttcga tcaagagctt tcgcaagccg agcaagccgc gttttccctc tcgcgcaagc
     1441 ggaccgaaac ggcatcacgg ctggcgcttg ggataagcga acagctcgcc agcctgggca
     1501 tgggcggcgc tcaggttcgc gtcgaagtgg cacgccacgc tggcgaagga gacctcatgg
     1561 tgaaaggcgc gaggttaacc gcggagggcg tcgaccgcgt cgagtttctc atcgcgccca
     1621 acaaagggat cgagccccgt cccctgagga aaatcgcgtc gggaggtgag ctttcacgag
     1681 ctttgctcgc catcaaaaga gtcctcgctt cccaaggccc cgccggactt tatatcttcg
     1741 atgaggtcga cgctggcgtg gggggcgccg tggccgaacg tatcggtcag gcccttgccg
     1801 atatcgccaa acatcaccag gtgttgtgta tcactcacct cgctcccatc gccgctctcg
     1861 cggactatca ctttgtcgtg cgaaagtccc aagacggtca tgtcgcacga acgcaagtcg
     1921 ttcccgtgac gggagaagac agagtgcgag aaatcgctcg gatgctgtcg ggaaaaaacg
     1981 taaccaaagc atcgatgcaa gcggccaccg aaatgatgca acagcgacag cgctaaatcc
     2041 gttcatcttc agcgatgtgg cctcggccat gaccggtgtc atgggcgaat ccagcgatcg
     2101 atcgcatgtt gttgcttggc ggaggccatc atgaccaaaa gaattttacg agacattatc
     2161 gaaatcgacg atgacctatg cgatggctgt ggactttgca ttccaagctg tgaagagggc
     2221 gccttggtcc tcgtcgatgg caaagtgagg cttgcggaag aagcccgctg cgatggcgct
     2281 ggcgcgtgtt tgggtcattg ccctcgagga gcgctacgcc tcattcagcg tgaggcggaa
     2341 cgattcgatg aacgatatgc ctcaggcatg cagccatcgg cgacgcaaag cgcgccttcc
     2401 cttgaatcac tcgttgcttt cggaaaaacc gccatacccc agcgtccagc ggcgatttcg
     2461 gctggctgtc ccggaagccg ctcgatggct tggaacacaa cgccctcttc ggtttcggtt
     2521 cccgcgaaag cggcttccgc gctgacacaa tggccgattc aactgcactt gctatcaccc
     2581 ggctcatcgt ttctgcgcgc acgggagctt ctgctcgccg cggactgcgt tgccttttcc
     2641 ctaggtagct ttcatggcga tcttctcgag ggaaggtcgt tggccattgc ttgccccaag
     2701 ctggatgacc cctctggcta cgaagaaaaa atcgctgaaa tcctcgctca ttgcgacgtc
     2761 ccggcgcttc gtgtggtcat catggaagtg ccatgttgcg gcgggctcct ccgcttggcc
     2821 tccacggccg ccaagcgcgc cggataccaa ggcgaaatcg aagtcatcgt cgtgtctcgt
     2881 ttcggtgaga tccttcaacg aaaatccctc acccccacgc catcacccaa cgaaagcacc
     2941 ggatgcgcgt gcggtggatg agcgcgacgc tcaaccgttg cagctcgtta cgtcaaaagc
     3001 ccataccgat gccgtggcac tcctagctga tcacacacca tgcgcagaat cgtttcggtt
     3061 tgccttcgta catcctgcag ggaaccttgg gtgtcgatca cgtaatcggc atgggatgct
     3121 ttttcgtgca gcggcatctg agcatcgatc cgcgatcgag cctgcgtttc cgatagcccg
     3181 tcgcgcccca tgagccgagc tatctgtgtg gccgggtcta cgagcgtcac aatcagtgga
     3241 cgatacttgt cggccaaccg attttcaacg atcagagcgg cttcatacac ggccaacgga
     3301 tgtccttgcc gcgcgagttg actcgcccgt tgttcggtcc gctggcgaat ccgtgggtgg
     3361 gtgatcgact cgagtctctt acgtgcatgc gagtcggcaa aaacgatggc tgccatttta
     3421 cgacggtcta gctggcccga ggggtcgagg acctcggtgc cgaaagcctc gacaatcatc
     3481 cccaatgctt ccgaatcggg ggccacgacc gcgcgcgcga tctcatcggc atcgaggatc
     3541 ggtacgccac gttcgctcaa aatggctgct accgcacttt ttccgctcgc gatgccaccg
     3601 gtcaggccca ggagcttgaa gctcatggct cgttccgttt ggattcgccc cgggtgatct
     3661 catacgcccg gatttttttg tgcaaattgg ttcgttcgac gcccagggca atggccgtcc
     3721 gcgagatgtt ccaatccaac gctcgaaggg tctccacgat atagttgcgc tcggcttgtt
     3781 cgcggaattc gcgaagtgta agacgtcctt cttccgactt ggaggccatc gaaggagata
     3841 tgggggaggc gtaactactg ctagaggcgc tattttctcc cagatcgtca tcgaacggag
     3901 agtcgtgagg atcttcggga agatcggcga tggtgatgag gtctcccgac aggatggccg
     3961 cgcgttcgat cacgtttttg agttctcgga cattgcccgg ccactttcgt cggcgaagcg
     4021 cgtcgtatac cggcgtgtcg atgctcatgg ggcgaagccc gttttctttg cagaaagcgg
     4081 tgacgaacga atccgacagc agacggatgt cttcgatacg ctcacgcaaa gctggacagc
     4141 gaatgggaaa gacagcgagt cggaagtaga ggtcttcccg gaatcggcca tcggccactt
     4201 ctttggacag atccttgttg gtggccgcga gcacacgaac gtcgacatgg atgagatgtt
     4261 cgcttcccac acgcgatatt tctccggatt gaagggcgcg aagcaccttg gcttgcgccg
     4321 tcaaatccat gtcgccaatt tcatcgagaa ataaggtgcc gccatgagct tgttcgaaaa
     4381 aacctcgctt tcgggtttgg gcacccgtga acgatcctcg ctcatgacca aacaattcac
     4441 tttcgatgag ttctcggggg atagcggcgc aattgacttt gacgaaaggg ctttccgccc
     4501 gggggctgag ccgatgcagg gctcggctga cgagttcctt gcctgtcccg ctttcgcccg
     4561 tgatgagaac actggctttg gtgggcgcca cccgatcgat ttcgcgatac agcttttgca
     4621 tcgaggggct cgttccgatc atttcgtaac gagcctcgaa ttgctcgcgc atgtcttcga
     4681 cggtgcgaac caggcgtgcg gttttcaata cgttgcgcac gctgaccaga acgcgctcgc
     4741 gattcaaagg cttttcgaaa aagtcgctgg caccgagctt gatggccgtg accgcatcgt
     4801 gaaccgtggc gtgaccggaa atgacgatga caggcagttg tggaaggcct tcttccgtgc
     4861 gcatgcgttc gagcatttcg agcccggaca tgcctggcag cttgacgtcc aacaggatca
     4921 aatccacggg gctgttgggg cgcgccaata ggccgagggc ttcttcggcg gaggcggctc
     4981 ccgtcacgcg gtagccttct cccgtgagca ccatttccac ggttcgacgg atgttttttt
     5041 cgtcgtcgac cacgagtacc atgggagcga cagcggtgag aggctggtcc tggggtgggc
     5101 aaggttcggt catggtttca cctcaccgga agatatctct tggagaaatg agcgagcggg
     5161 tacgacgaag gggctgtcgg gataggaggt gagcacccga tggaaagcct cgcgtgcttg
     5221 cggtcgctgt ttcatcttga gataggtttc tcccaataaa acaagggctt ccggttccag
     5281 cccggagttc tcatagtgct ggagagcata acgtgcgcgt gacgccgcgg cgggaaagtt
     5341 tccctcacga agataaaacc gcgccacgta tagctcgtga cgaaccaatc gcccggtgac
     5401 gtcgagcagc atatagtcga cctgctcgtc ccatcgactc gtcggatact cgcgcttgaa
     5461 acgtcgaagc tcctgatagg cctccgtggt gggaccttgg tctcgctcct cctgcggagg
     5521 aagcatgatc gtatcgttga tttgttgaaa caaggcttgg caaactcggt attgcgcata
     5581 cgcgataccc gggtccaatc gtcgggtctg cacgaaggtt ttgtaggcgc tgatcgcttc
     5641 ggggagttgg tcccgctcga acttgatgtc cgcgagtcgc aattccgccc atcgggcata
     5701 ccggctctgc ccatattccc tgcgtacttt ctgaaaccgt ttctccgctt cttcccagtc
     5761 ccgttcgtaa aacgagcgca tggctttttc gtaaaataaa cgggcctctt cgctgtattg
     5821 cagcgtgccc ggacgagcgg caggcaactc cttgctacag cctgtcgcca tcgtgacgag
     5881 cagcactgtc gcgagcagag ccaacaacct ttgcagtatg ttcatcgccg actttcccta
     5941 gccctggacc gtctcgatct caacggtgat gatgactcga cgaggttgcc aaggaaaacg
     6001 aaggttgaat catctccctc gcgacggccg gcgatttctc ctaagcttcg ggggtgacat
     6061 ctcgcagcca tccgataatc ctcgaagccc agttttctta tggttttacg gaccttgggt
     6121 ccgctccgcc cagtacatgg cctgaaatcg ccttcgcggg acggtccaat gttggcaaat
     6181 cgagcttgct caatgctctc ctgcaacgga aaaacctcgt tcgcaccagt tcgacaccgg
     6241 gctgtacgcg ccaaatcaac gcgtttctgg ttcgtttgga acatttgggc ccgctgcacc
     6301 tcatcgactt gcccggtttt ggatttgcca aacgctcaaa aaccgaacga aaacaatggg
     6361 gtagcatgat cgaccagtat ctgcgagacc gcgccgctct tgcgggggtt gtcgttctcg
     6421 tggatgttcg ccggggcccc gaatccctcg aagcaagcct cattcagtat ctgcaggaaa
     6481 tacgcgaacc tgcgattccc tatcttttcg ttgccaccaa agtcgacaag ctgcctgctg
     6541 ctcaactcaa gccaagactc gatgccttgc ggaaaacgac acccggacgg attatcgggt
     6601 tttccgccaa aaccggtctg ggccgcgaag agctttggaa gcaactcctc gaggcaattc
     6661 cagcatccaa taggcagaag gcttgcctct gaagcaccaa taaagtttgt cgatctcgtt
     6721 ggttctttgg taaagtggcg gctcgagggt taagtggcga ccattcagct tgaccaagaa
     6781 atcctggatg tcgagagaat catcgacgaa aaaaaccatg atgaggcttt tcgtcggttg
     6841 attctcttgc gcgaccgatt tgggcgtcga cccgaatatc ggtacttgaa aattctgttc
     6901 gatgccacgt ttgccgtccg aacggatgcg gaactccttt ctgaggtccg aacgctcgtg
     6961 gccgaacagc aagactttct ggaagcggtg gcgttgctcg ctttgctgct cgaccgcacg
     7021 ggcgaccatg agcgcgctat cgtgtttgcg agggaagcac tgcggtcacc taacagccgc
     7081 gcgcaaatgc gggcgcgagc catcttgggg gagcaggcag agcaggaagt cggcggtcca
     7141 cccccatcgg tgaatcgatt tgccgcttcc accgcacggg aagcccccac caaaccgcat
     7201 gtcaggccgg tcaatgtgcc cgcggccgac ggacaaagcc aaacccgttc caccaaagcc
     7261 ctgtttgcgg tcgccgacga caaacaaagt tcacccccgg tggataaggc ggaagccaaa
     7321 gaagcggact tgaccctgga aggcgtggcg ccgccttcca acctcgatgc ggcgtttgcc
     7381 gccgttcgcc ctcgtgaacc gaattcttcg aacctccggc tgccgagcag ccccccgatt
     7441 caggacgatc gagtcagtac cctcgaaggg ctccctaggc ccccgttggt gcctagcaag
     7501 acgttttcag gcgcgcaaca gccgcccgat tctcttcctt ccacggtcga cttccgaatt
     7561 tccccgtcac ttcccgagca ggagccgagc caaaacagcg ttggggccga gttcattcct
     7621 ttttctcctg accaggcacc catgcctcct ccccccacga tcccaccggg cgtcgtgaca
     7681 gccaacgaaa ggagtcgtcc gccaactccc cacccatcgg ctccgcacgc gcaccgttct
     7741 tcgttttcgc aagcatctac cccaccgccg cgtcggtcgg cctttccgcc atcgcccgag
     7801 cctcgcgcgg atatcgcacc ggaaaccact gcttctcgtc tcccctccac cccgccgacc
     7861 cgacgacaac ccaccccgtt gcctcccgaa tatgccgttg acgatacggg tgtggggcgt
     7921 gaggtcgagc gacgacgttc ccggccttct cgtcaagccc tttctgtgcc tcagcctccc
     7981 gagcacatca aagggtggtt ccaattcgca cggcaaaatc agctccaggt agtcgagggg
     8041 ttttccacgg cccagatgct gctcgactta tgtgaacggg tcgtcgaagg ggccacttct
     8101 ttgtcgagtc gtcctgttcc gctcgaccgt cgtgggctca ttttggtgga agaacaactc
     8161 gaagcgttgc gaggttcgcg aaccaaaacc caaccggttg ccgaacgcgg cgccgttacc
     8221 gccgccgctt catttttgct cgggttgttg ctcaaagaat gcgatggtcg cgccgccgat
     8281 acctccgtcg aagacggagc ctgtaaagta acattgcctt ccggggctac tgtccgaccc
     8341 ttgctcgtcg ccgctgcttt tatgcgttcc cgcggcccaa gcctcatcga gactttcgac
     8401 cgggcggcca cctcgcatat gcgacgaaac ccagctcgcg tgacgacagg caatgcggct
     8461 cgcgtcatca cgggcaatgc ggctcgcgtc atcacgggca atgcggctcg cgtcatcacg
     8521 ggcaactcca tttcccaacc gccccttcga aacgcgcgca ctcccgccat cgctgccgct
     8581 tccagcttca cggtgcttcg cagggatctc gacgccagca ccttgggcat tacggatccc
     8641 gcggctccgc cggcgccaca accaccgata gacatgcgca aagtggcctc cgagttctgg
     8701 accagcgacc tcggacgaga aatcattggt agttcccgta gggtaggcac tttcaccatc
     8761 gccgacgtgg acgccatcga acgtcatgcc agcaaagctt tcaacgcggt tgggttctca
     8821 cctcctggat ctccgtggcc gtggatgccc tcggaggaac aggatgactt gattttggct
     8881 tggggggcca tcctcgggga agtgctcgtt tcgttatatt tgggacgttg ggaagcggat
     8941 cccggcaatc ctgatgaccg tttcctgttt cgcgtgatcc tcagcagtgg tgtcgttgcc
     9001 tggcctgtcg ccaaagccta tatgcgctta gcccgcggaa ttcctcatga tttgaccgta
     9061 tatatcgatg cggtggggcg tgtggtcggc aggcaagcgc tgggtgctcg ctcctggcaa
     9121 gggctttgac cggcttatcg tcccggttcc tgccattgtg ttgcattagg cttgtccgag
     9181 gcatgcccta tcattggccc gcacgttgtg gtatgcggcg ccgccgttgc gaacaccgct
     9241 tttgcaaaac cttttgcccg gaacgaaaaa gcctttcgtt acgcccgtac ggcatccatg
     9301 agccatgact gcccgttgtt caagccgata ccgtctattt gtagccaaag acgagcataa
     9361 gtttagctgt gctcacatga ccttatttcc tgatggaacc aaagagcgtc tgcacggcca
     9421 taattatcgt gtcggggcgg cgttggagat gcaggaaggg tttctcgact tcgcgcgggt
     9481 caagtccgca ctggcgacat tgtgtgaaga attgcgggaa catctgttga tgccttccca
     9541 gtccgcggcc gtgcgtgtgc tgagccaaga cgaaatctcg acggagtttt cggtatgcgg
     9601 aaagcgatac gtcattccat cggatgaatt gttgtggctt ccgatgcaaa acgtggtggt
     9661 ggagggcttg gcggaatact tgtgggggcg aatcgcccag caccttcggg cggagtttga
     9721 gcaagcgggg gtggggtggt tggaggtgac ggtgaccgaa gcgccggggc aaggcgctcg
     9781 cttccgtgcg gatcctcgga aaggtggata agcgatggct ttggataagg tcatggtgcg
     9841 cgccattcgc gtggatgctt cgattggcac gctttcatgg gaaaagcacg agcttcagcc
     9901 ggtgcaagtg gatgtggacg cggtggtgga taccgaggcg ctgcttggtt cgggggattt
     9961 gaccaaaggc gtcgactttt cgatcgtgat ccaaaccgtg cgcgatgttg tcacgcaagg
    10021 gcatgtcgaa ttggtcgagg tgttggcgga tcgaatagct tcggcactgc ttgccgcgac
    10081 acccgcgcga agcgttcggg tggaagtacg aaaatacaag gcctgtgcag agtacgcgtc
    10141 ccatgtggga gtcgaggttc atagagagcg gacccatggc tgaaaacctg tctgtgttgg
    10201 tggtcgatac ggccacccga cgagcgatcg tggcgctggt gcggggggga atcgtggtgg
    10261 cggaggcgtt ccatgagcag ccaacgacgc atgcggagcg gttggtggcg atgacgacgg
    10321 aggtattgga gcgttctggt gcggggtt
//
LOCUS       DAOSOR010000152        10277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6510_length_10277_cov_4.317942, whole genome shotgun sequence.
ACCESSION   DAOSOR010000152 DAOSOR010000000
VERSION     DAOSOR010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 10277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10277
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6510_length_10277_cov_4.317942"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /gene="alr"
                     /locus_tag="PK710_10160"
     CDS             complement(<1..897)
                     /gene="alr"
                     /locus_tag="PK710_10160"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOT10124.1"
                     /translation="MQRPRRATPADAVRPTRAEVNLDHLRHNLRVLRRLSGGTRLWGV
                     LKADGYGHGAPAVARTLERSGIDGLCVALLEEAVELRNAGIHAPVLVMGGNYGHGWGE
                     LLAHELTPVIYDREQLEALAKEVHLYGFAPIGVHLKVDTGMSRLGVRHDRLGPMLDRI
                     RDLPEVRLTGMMTHFANADDDEMEATQHQCALFERAIDEVRAAGFHPEVIHAANSAAL
                     IRLPRARYDLARPGIALFGVSPRADLIADLRPVMRVRSEVVALREVLPGEGAGYGSSW
                     KARRISRLATVPMGYADGLPRSL"
     gene            complement(1000..1608)
                     /locus_tag="PK710_10165"
     CDS             complement(1000..1608)
                     /locus_tag="PK710_10165"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF003296.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOT10125.1"
                     /translation="MVAEFNPFPWLSSPSIEPRGHIEVVTGGMFSGKTEELIRRLRLA
                     MHAKRKVQAFKPRIDDRYDATKVMTHFGVGIEAVAVPNSAALEARVEADTQVVGVDEA
                     QFFDAGIVDLVERLADRGVCVLLAGLDQDYLGRPFAPMPQLMAIAEHVTKVYAVCAVC
                     GAPATRSQRIVEQKSTILVGGDESYEPRCRCCFQRRELSRTS"
     gene            1806..2057
                     /locus_tag="PK710_10170"
     CDS             1806..2057
                     /locus_tag="PK710_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10126.1"
                     /translation="MLVVLRKLGRGSRAVTGRLVRAPRKGSVIVVEFSNGLHEYVTTP
                     VRRILRVAGQDVFFIETVNSRYRLEICGRGQDLEGASSG"
     gene            2200..2415
                     /locus_tag="PK710_10175"
     CDS             2200..2415
                     /locus_tag="PK710_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10127.1"
                     /translation="MAKNDRRNSRKMKRRRGEASKRRTQARRKALVTEARKNGTKVKK
                     LTPSALPPKVDAASTVTPETETSPTEA"
     gene            complement(2429..2935)
                     /locus_tag="PK710_10180"
     CDS             complement(2429..2935)
                     /locus_tag="PK710_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10128.1"
                     /translation="MGPFHHKRSVRHVVSNMASVGLLLLAVGCMGQTKMAKLQEAASD
                     LNMATRFGRMDVATERVSPKDLEGFARRHAAWGGSIRIVDVEYRGIQIVDDNTAVVFV
                     AVGWQQPDDPNLRVTQLAQQWEYGQGGWKLKDETRNAGDVGLLGETTEYLRPESRPDV
                     HYPSITIR"
     gene            3016..3948
                     /locus_tag="PK710_10185"
     CDS             3016..3948
                     /locus_tag="PK710_10185"
                     /inference="COORDINATES: protein motif:HMM:NF013514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10129.1"
                     /translation="MSIAVGIDENGLGPQLGPLVVTAVTAKVTAQAEQRMTHDPTVFF
                     HDRLADSKKLVSHQKVQLAEAWARAIAGPAACPDALLQHLCLDSQAVRHSLCPDSALP
                     QCWSPHDEAFQASENQVCLARNDLKDLRSLGVDVLDARCVILCVRRLNDGRHRGVSRL
                     DFDLQAMESLILAIVQHTQQPIDARCGKVGGMMRYPSKFTHLSGFPYGVLHEQRSESA
                     YRLAGIGTIRFLQDAEEKDPLVSIASLVGKWVREMLMNRIVRFYRQHDDTLPDASGYH
                     DPVTNAFVRNSQALRQRLGIPQDCFLRQGKKDVC"
     gene            complement(4051..5565)
                     /locus_tag="PK710_10190"
     CDS             complement(4051..5565)
                     /locus_tag="PK710_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10130.1"
                     /translation="MSTTNTRPPIIRAVRVRPTVKALALGCLLFSGCSDDDGDSNLGG
                     SAGSQDASAGGSSLSEAGPESASSGSGGTSGSGGTSGSGGTSASGGASGSGGTSASGG
                     SGGSGGASQPVCGDGLCDKTETAGNCPADCAPCQPLPPNATPIEKLLYELPPDTWYEM
                     PNTPMRAVCPPKSEVGGVGDCEMVVRAWGGGALDTKRNRLILWGGGHNDYWGNELYAF
                     EVATGTWKRLTDPSKGTVTNQDPLPDGNPVSRHTYDGIDYVHHADVLFAQGGSRASDG
                     GGTDVTWTFDFVTSAWTNHAPDPKGPGGYCIATAYDPNSKSIFARSTKNLYIYDLESN
                     VWTPLVGFGYTPLWPRYERWGDKTGTVDPKRHLFWSVGNGDILVWSIDEKKLVTEDWV
                     TTGGGDYSNAGYVKGYPEQLFESGGGAVYNVDAPGFDYDVQADALVGWPNAGSPYVLD
                     LTTKIWEKGSDQGAPTSKTSGGTYGRFRYIPAYNVFILVVSVDKNVFFYKNRGC"
     gene            complement(5576..7786)
                     /locus_tag="PK710_10195"
     CDS             complement(5576..7786)
                     /locus_tag="PK710_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10131.1"
                     /translation="MKRSFFFLWLTASWAMGWMGCGDSDSADSNSSTPTESDAGSDVV
                     SEGSTGTDALSDGGSDDTASEAAMEGSTPLGSVITTVNLHNPTEKEAIDVPLTFGHVF
                     RPGDVPKGTTLQARVGETLIPTQVDSKARHADGSLRHAIVTVVASIPASGAVQAELLT
                     IPLPEAQTAVELGDLLNTDFNASLTLTVDAKTYSLDAKTLLQGKIDRTWLSGPIVSEW
                     IVAGPVVSGQSYHPHLSARFSVRAYTGLKSVRVGLSVDNDWAFEPEPRNFVYDASVQM
                     GSKQVFSLSNLTHHRQARWRKVFWWGDEVQAYVTHDSRYLMNSGALPHYDPELTISDE
                     GLEKQYAKWGTLNTGPMGIGPMEPYMPTTGAHDDIGPLSRWDALFLCSMDRRAQEMAM
                     GLADLAGSWPIHYRDKDTGMPVRLDDYPYMSLLGNPGDKVNPKTGKSEAFPACASQAD
                     CKSPYTPDSAHQPSMGYLAYVVGGDYDHLEELMFWANYNMLESNPHYRAFEQGLLKWG
                     QVRGQAWSLRTLGFAAYIVPDDHSFKAYFNERLDYNLQYYLDRYTKNEPNPLGIFTDG
                     YAFAYNEGRGIAPWQDDFFTWSVGQLVAMGFPKAVPLLEFKAQFPVGRMTAPGFCFIH
                     AAAYQMNVRDEKGAPIYDTFAEVYQKTVDPAIQALPCASAEMGEALSLAPGELTGYSS
                     STAGYPSNMQPALAVAVESGIPNAKDAWETFAQRPVKPDYSAYPNWAITPRIQP"
     gene            8055..8258
                     /locus_tag="PK710_10200"
     CDS             8055..8258
                     /locus_tag="PK710_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10132.1"
                     /translation="MARRGKRDCGTQHRAGTGQVRQSFGSFVRRSLHRSTMLPDPRYG
                     GTVACCEVLAKDPVMFLAYRGDT"
     gene            8449..9888
                     /locus_tag="PK710_10205"
     CDS             8449..9888
                     /locus_tag="PK710_10205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOT10133.1"
                     /translation="MTSGRVDRATCRILEGPLAWEIARFGAPLALAMGLQVTFNLVDA
                     YLIARLSPEVAGPALGAIGICDQIAALGTILSYGISIATAALLSQYQGRGDTQAVRKV
                     AAQSMWLVLGLSGLFGLAGWLWAEPVIVDVVGAKGAVATMSIQYLRVIVGGSFSIFLL
                     LQLTSMQRALGSSKTPVMILLGSNALNLVLAVLFCYGPGEAPEIFSWGPPIAKALHIP
                     RMELVGAAWATIGARVVFLIPLIFLIERRFHLFRGHLSLPRDTRVLRSITRIAWPAST
                     QFVVRITAMLLTHSLVARAFTTAEDQHASTALGIVFRLETLALFIAMGWGSAAQTFLG
                     QNEGAGQHRRALDSGWYATAYNAVAMAGLAVLFYLAGDAIVRFFDSDSAVVDMAVQYL
                     RVVSPFYLALGVGIVLGNAITGAGATRLTLTIDSLVVLLVQLPLSLWAVHHAQTPERL
                     WAVLAGVHVAFACVYGFVYRKGHFLRRLA"
     gene            9932..>10277
                     /locus_tag="PK710_10210"
     CDS             9932..>10277
                     /locus_tag="PK710_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10134.1"
                     /translation="MEFPSDTTVRWLVHRTAALLTRQQDPNVKRPVVLPNGEFFPDDF
                     DGDAGSVNLLFWRLQEHGKLTDLDVELRFIDDGGAASGAGCCSAGSCGTQGNAVAPAP
                     LPPVVRLSDAYRV"
BASE COUNT         2173 a         3198 c         2737 g         2169 t
ORIGIN      
        1 caagctccgc ggcaaaccat cggcatatcc catgggcacg gtagcaagcc gtgatattcg
       61 tcgcgctttc cacgaggaac cataaccggc cccttcgccg ggaagtactt ctcgcagcgc
      121 cacgacctca cttcgtacgc gcatcacagg acgcaagtcc gcgatgagat ccgctcgcgg
      181 ggagacacca aatagcgcga tacccggacg cgccaaatca tagcgcgcgc gaggaagtcg
      241 gatgagcgcc gcgctgttgg ccgcatgaat gacttcggga tgaaatccag ccgcacgaac
      301 ttcatcgatg gcccgctcga acagcgcgca ttggtgctgc gtagcctcca tctcatcatc
      361 atccgcattc gcaaaatgag tcatcatgcc cgtcaaacgc acttcgggca gatcacgaat
      421 tcggtccaac atgggcccca aacgatcatg cctgacaccg agacgagaca tgcccgtatc
      481 caccttgaga tgcactccga tgggcgcgaa cccataaaga tgcacttctt tggcgagcgc
      541 ttcgagttgc tcccgatcgt agatcacggg cgtaagctca tgcgccaaca gctctcccca
      601 gccatgacca taatttcctc ccatcacgag gacaggcgcg tgaattcccg cgttacgaag
      661 ctcaaccgct tcttccagca acgccacaca taacccatcg atgccagacc gctcgagggt
      721 ccgcgccacg gccggtgccc cgtgtccgta accatcggct ttcaataccc cccataatcg
      781 cgtgcctccc gacaaacgtc gcaaaacacg taaattatgc cgcaaatgat caaggttgac
      841 ctccgctcgg gtcggtcgaa cggcatccgc gggtgtggct cgtcggggtc gttgcacgcg
      901 aggtacagga gtcagggctg gtatcgccat agttatcctt ccgcgcttat cgaacgccac
      961 agctctggtc aagattcctg cagttcgaaa gatcgaccgt cacgaggttc gggacaactc
     1021 tcgtcgttga aaacagcaac gacaccgagg ctcatacgat tcgtctcccc ccaccaaaat
     1081 cgtcgacttc tgttcgacga tccgttggga tcgcgtcgct ggcgccccac aaaccgcaca
     1141 gaccgcgtag actttcgtca catgctccgc gatggccatg agttgcggca tcggcgcaaa
     1201 tggacggccg agatagtctt ggtccaagcc cgcaagcaac acacaaacgc cacggtcagc
     1261 caaccgttcc accaagtcga cgatccccgc gtcgaaaaat tgggcttcat ccacgccaac
     1321 cacctgcgta tcagcctcga cccgcgcttc caaagccgcc gaattcggca ccgccaccgc
     1381 ctcgatccca accccaaaat gcgtcatcac cttcgtggca tcatagcggt catcaatccg
     1441 tggcttgaac gcctgcacct ttcgtttcgc gtgcatcgcc aaacgaagcc gtcgaatgag
     1501 ttcctccgtt ttgccgctaa acatgcctcc ggtaaccacc tcgatatgac ctcggggttc
     1561 gatggaggga gaagacagcc atggaaaagg attgaactca gcgaccacgg cgcggcagca
     1621 aagcgccgct cgcacggaaa cgcaagagac ggaatgagat ttgacgaccg cgggtagcca
     1681 accttcccac gcgccccccc aacccctccc acgcttgccc aatccgctca tgctccattt
     1741 gccgacttgt atcgattttc aatcgttgcg gtggacgaac ccagggttgc tgctacgata
     1801 ggggggtgct ggtcgtatta cgcaagctgg gacgtggctc gcgagctgtg accggtcgcc
     1861 tcgtgcgagc gccgaggaaa ggatccgtca tcgtcgtcga gttctccaat gggctgcacg
     1921 agtacgtaac caccccagtt cggcggattc tccgcgtggc aggacaagac gtcttcttca
     1981 tcgaaaccgt caacagccga taccgactgg aaatttgcgg tcgcggacaa gatctggaag
     2041 gcgcctcctc cggttgagcc ccctgctagc aaccggctcc gatgcacccc ctcataggaa
     2101 actcttgatt ttcacaacgc gctggaccaa aagaggcaac tcttcgcaac cgcgtcgctt
     2161 gcgacctgcg aaaatgccca cgctcgagag gattgcctca tggccaaaaa cgatcgtcga
     2221 aattcccgga aaatgaagcg ccgccgcggc gaggccagca agcgccgtac acaagcccga
     2281 cgcaaggccc tggtcaccga agctcgcaaa aacggcacca aagtcaaaaa gctcacgccc
     2341 tcggccctgc cgcccaaagt cgatgccgca agcaccgtga cgccagagac cgaaacttcc
     2401 ccgacggaag cgtaacttat tgattatcct agcgaattgt aatgctaggg taatgcacgt
     2461 ccggcctgct ttccggcctc aaatactccg tggtctcccc caatagacca acgtcacccg
     2521 cgttgcgtgt ctcgtctttc aacttccatc ccccctgccc atactcccac tgctgcgcca
     2581 gttgcgtcac acgaagattg ggatcatcgg gctgctgcca ccccaccgcc acaaacacaa
     2641 ccgccgtatt gtcatccaca atctgaatcc ctcgatactc cacatccacg atacgaatgc
     2701 ttccgcccca agccgcgtga cgcctcgcaa aaccttccaa atccttggga gatacacgct
     2761 ccgtcgccac atccatccgc ccaaatcgcg tggccatatt caagtccgaa gctgcctctt
     2821 gcaacttcgc catcttggtt tgccccatgc atccaacagc cagaagcaac aacccgacgc
     2881 tggccatgtt ggaaacgacg tgtcgaacgg aacgcttgtg atgaaaggga cccatgacga
     2941 ccttcctgta tcagccttgg gcccatgggg aaaagttcgc ggcacctttc cccaacgtgt
     3001 aagctcccct atcccatgtc gatcgctgtc ggtatcgatg aaaacggact cggcccacaa
     3061 ctcggccctc ttgtcgtaac cgccgtcacg gccaaggtca cagcccaagc cgaacaacgg
     3121 atgacacacg accccacggt ctttttccat gatcgccttg ccgattcgaa aaaactcgtg
     3181 agtcatcaaa aagtacaact tgccgaagcc tgggcgcgcg cgatcgcagg acccgccgct
     3241 tgccccgatg ccttgctcca acacctctgc ctcgatagcc aagccgtacg tcattccctc
     3301 tgccccgatt cggccctgcc ccaatgctgg tcccctcacg atgaggcgtt ccaagcatcc
     3361 gaaaaccagg tttgcctcgc acgaaacgac ctgaaggatc tgcgttcgct cggcgtcgac
     3421 gtgctcgatg cacgatgcgt catcctatgc gtgcggcgtc tcaatgacgg acgacatcgt
     3481 ggagtgagtc gtctcgattt cgatctgcaa gcgatggaat cgttgatcct tgccatcgtc
     3541 caacacaccc aacaaccgat cgacgcgcgc tgcggaaaag tcgggggcat gatgcgttat
     3601 ccctcgaaat ttacacacct ttccggcttt ccttatggag ttttgcacga acaacgatcc
     3661 gaaagcgcct atcgtctggc agggatcgga accattcgct ttttacaaga cgcggaagaa
     3721 aaggatcccc tcgtgtccat cgcttccctg gtaggaaagt gggtgcggga gatgctgatg
     3781 aaccgtatcg tccgcttcta tcggcaacac gacgatacct tgccggatgc aagcggatat
     3841 cacgatccgg tcacgaacgc attcgttcgc aactcccaag cgcttcgaca acggcttggt
     3901 attccgcagg attgtttcct tcgccaaggg aaaaaagacg tctgctaacg agaagcgcgt
     3961 cgtggcgggg ttgaaggcca aggagcccac acacggcatc aaataggtcg cccggccaag
     4021 gaccaacctt gacgagcggc aaggggagat tcagcaaccg cgatttttat agaaaaatac
     4081 gtttttgtcc acactgacca cgaggatgaa aacgttatac gcgggaatgt agcggaaccg
     4141 gccataggtg ccaccgctgg tctttgacgt tggggcgccc tgatcgctgc ctttctccca
     4201 tattttcgtt gttaagtcga gaacataagg acttcccgcg ttcggccatc ccaccaacgc
     4261 atcggcttgt acgtcataat caaatccggg ggcatcgaca ttatataccg cccctccgcc
     4321 gctttcgaaa agctgctccg ggtagccctt gacatatcca gcgttggaat aatcgccccc
     4381 gcccgtggtt acccaatctt ccgtaacgag ctttttttca tcgatgctcc acacgaggat
     4441 atcgccattg cccaccgacc aaaacagatg ccgtttggga tctaccgttc ccgttttgtc
     4501 cccccacctt tcgtagcgcg gccataaagg cgtatagccg aagccgacaa ggggagtcca
     4561 aacattggac tctagatcgt atatgtataa attcttggta ctgcgcgcga atatcgactt
     4621 cgagttggga tcatacgctg ttgctatgca gtagccgcca ggtcctttcg gatccggggc
     4681 atgattcgtc caggccgacg tcacgaaatc gaatgtccac gtcacgtccg tgcccccacc
     4741 atcactcgcc cgcgacccgc cttgtgcaaa caacacgtcc gcatggtgga cataatcgat
     4801 gccatcgtag gtatggcggg atacgggatt accatccggc agcggatctt ggttcgtgac
     4861 cgtacctttg cttggatccg taaggcgttt ccacgttccc gtggccactt cgaacgcata
     4921 tagctcgttt ccccaataat cgttatggcc tcctccccaa aggatcaatc gattgcgctt
     4981 ggtatccaat gcgcctccgc cccaggctct cacaaccatc tcgcaatcgc caacgccccc
     5041 gacttcggac ttgggggggc agacggcgcg cattggcgta ttgggcattt cgtaccacgt
     5101 atcgggggga agctcgtaaa gaagcttttc gataggggtg gcgttgggag gcaagggctg
     5161 gcagggggca cagtctgcag ggcaattacc tgcggtttct gttttatcac ataagccatc
     5221 gccacaaacg ggctggcttg ctccaccgct tcctccactc cccccacttg cgctcgttcc
     5281 cccgctgccg ctcgctcccc cacttgcgct cgttcccccg ctgccgctcg ttcccccgct
     5341 tccgctcgtt cctccgcttc cgctgctcgc tgattccggg cctgcttccg aaagagatga
     5401 accgcctgca cttgcatctt ggctgcctgc agaacctcct agattggaat ctccatcatc
     5461 atcggaacaa cccgaaaata acaagcaccc caaggccagt gctttcaccg ttgggcggac
     5521 ccttacggcg cggattatcg gcggacgggt gtttgtcgtg ctcatcgcat cctcctcacg
     5581 gttggattcg gggtgttatg gcccaattgg gataggcgct gtaatcgggt ttcacagggc
     5641 gttgtgcaaa ggtttcccac gcgtctttgg cattcggaat gccagactcg accgccacgg
     5701 ccagcgccgg ttgcatattc gaaggatacc ccgcggtgga gctggaatag ccagtcagct
     5761 ccccaggggc aagcgaaagc gcttcaccca tctccgcact tgcacagggt agcgcctgaa
     5821 tcgccggatc gacggttttc tgatagactt cggcaaacgt atcatagatt ggagcgcctt
     5881 tttcatctcg tacgttcatt tggtaggccg cagcgtggat gaagcaaaaa ccaggggccg
     5941 tcatccgccc caccgggaat tgcgctttga actccagcag cggaacagct tttggaaacc
     6001 ccatcgccac gagttgcccg acagaccacg tgaaaaagtc gtcttgccaa ggagcgatcc
     6061 cccgcccttc gttatacgca aacgcgtatc catccgtgaa gatacccagg ggattgggtt
     6121 cgtttttcgt gtagcggtcg aggtagtact gcaaattata atcgagccgc tcgttgaaat
     6181 acgccttgaa cgaatggtca tcggggacga tatacgccgc aaatcccaac gttcgtaacg
     6241 accaggcctg cccgcgcact tggccccact tgagcaatcc ttgttcgaaa gcccgataat
     6301 gaggattgga ttcgagcata ttatagttcg cccaaaacat gagttcctcg agatggtcgt
     6361 aatcacctcc caccacatac gcgaggtacc ccatcgaggg ttgatgagcg gaatccggcg
     6421 tgtacgggct tttgcaatcc gcctgcgacg cacaagcggg aaacgcttcc gacttacctg
     6481 tcttcggatt taccttatcc cccggattgc ccagcaaact catataggga tagtcatcga
     6541 gacgcaccgg catgcccgta tccttgtcgc gatagtgaat cggccaactt ccagccaggt
     6601 ccgcaagtcc catcgccatc tcctgcgcac gacgatccat gctgcaaagg aaaagagcat
     6661 cccatcgaga cagcggaccg atatcgtcgt gggccccggt cgttggcata tacggttcca
     6721 tcggtccgat ccccatcggt cccgtgttca acgtacccca tttggcatat tgcttttcca
     6781 atccctcgtc ggaaatcgtc aattccgggt cgtaatgggg caatgcgccg ctattcatca
     6841 aataccggct atcgtgcgtt acgtacgctt gcacctcatc gccccaccag aacactttcc
     6901 tccagcgcgc ttgacgatga tgggtcaggt tcgacaggga aaacacctgt ttgctgccca
     6961 tctgcacgga ggcgtcgtac acgaaatttc gaggctcggg ctcgaaagcc caatcattgt
     7021 ccaccgatag gccgacacgc acgcttttca gccccgtata cgcgcggacg gagaatcgcg
     7081 cggaaaggtg gggatggtaa ctttggccgg aaacgacagg gcctgcgacg atccattcgc
     7141 tgacgatcgg ccccgatagc caggttctgt cgatttttcc ttgcaacaag gtcttggcgt
     7201 cgagggaata ggtttttgca tcgaccgtga gcgtgaggga agcgttgaag tccgtgttca
     7261 acaagtcccc gagttcgaca gccgtttgcg cctcaggcaa cggaatggtc aataattccg
     7321 cttgaacggc ccccgaggca ggaatgctag ccacgaccgt cacgatggcg tgccggagcg
     7381 atccatccgc atgacgtgct ttactatcga cctgggtggg aatcagtgtt tcacccacgc
     7441 gcgcctggag cgtcgtccct ttgggaacgt ctcccggacg aaagacgtga ccaaaggtca
     7501 agggcacgtc gatagcctct ttttccgtgg gattgtgaag gtttaccgtc gtgatcacac
     7561 tccccagggg tgtgcttccc tccatggccg cctcgctcgc ggtatcgtcg gagccaccgt
     7621 ccgaaagggc gtcggtgccg gtgcttcctt ccgaaaccac gtcggaaccg gcatcggact
     7681 ccgtgggcgt ggagctgttg gaatccgcgg aatcactatc cccacagccc atccatccca
     7741 tcgcccacga cgccgtcaac cacagaaaaa agaagctacg tttcatgctc tcctcattgg
     7801 aattcttcag aatttctaaa cggacagcaa aagcaatgcc aggccgtcga gcgagcccac
     7861 gcagagaatg ggtatgatgc tggatctggg atgtcccaca tcctgaagag atgggaaaag
     7921 cagtgcgccc aggttgtcgc agggcgaagg cgggcaagcc ccagatcggc tgtagcgact
     7981 tcacgcaaga aacggcacag ccgcggtaag cacgaggata atgatgatga ggcgcaccat
     8041 gaggtacgag tctcatggca cggcgaggca agcgagattg tggaacgcaa caccgagcgg
     8101 ggacggggca ggttcggcaa tcatttggct cgttcgtgcg tcggtccttg catcgttcga
     8161 ccatgttgcc tgacccgcgg tacggtggaa cggtcgcttg ttgcgaggtg ctcgcgaaag
     8221 atccggtgat gtttcttgct tatcggggcg acacgtgagt acggaaggca aaatgcttcg
     8281 gatcgcacgc gtttgtggaa ataacgatga aggtttgccg gtgagaagcg gcggcgaggc
     8341 acgcaacatg aacgctgcta tatgcgaatc gaaagcgatg gatcgcggaa atggcagtcg
     8401 gacgggcact cgaaggggag attgccttgc cgaggagaaa aaagatcgat gacatcgggc
     8461 cgggtggatc gggcaacctg ccgcatcttg gaagggcccc tggcctggga aatcgcacga
     8521 tttggagcgc ctttggccct tgccatggga ctgcaggtga cgttcaacct ggtggatgcc
     8581 tatctgattg cccggttgag tcccgaagtg gccggacctg ctcttggagc cattggcatt
     8641 tgcgatcaga ttgcagcctt gggcacgatt ctttcctacg ggatctcgat cgcgaccgcg
     8701 gcgctgcttt cgcagtatca gggccgtggt gacacccagg ccgtgcgtaa ggttgctgcg
     8761 caatcgatgt ggttggtgct tggactcagt gggctattcg ggctggcggg atggttatgg
     8821 gccgaacctg tgatcgtgga tgtcgtggga gccaaaggcg cggtggccac gatgagcatc
     8881 caatatctgc gggtgatcgt gggcggaagc ttctcgatct tcttgctcct tcaactcacg
     8941 agcatgcaac gggccctggg ttcgagcaaa acgcccgtca tgattctcct tggctccaat
     9001 gccttgaatc tggttctggc cgtgttgttt tgttacggac cgggagaggc tcccgagatt
     9061 ttttcttggg ggccacccat cgccaaagcc cttcatatcc cgcgtatgga actggttggc
     9121 gcggcctggg ccaccatcgg cgcccgcgtc gtatttctca tcccactcat tttcctcatc
     9181 gaacgacgat ttcacctatt tcgaggccac ctatccctcc ctcgcgatac tcgagtatta
     9241 cggtccatca cccgcatcgc ctggcctgcg agcacacaat tcgtcgtacg catcaccgcg
     9301 atgcttctca cccattcgct cgtggcaagg gccttcacca ccgccgaaga ccagcacgcc
     9361 tccaccgcgc tcggcatcgt ctttcgcctt gaaaccctgg cgcttttcat cgccatgggt
     9421 tggggaagcg ccgcccaaac gtttctcgga caaaacgaag gcgccggtca acatcgtcgt
     9481 gcgctagaca gcggatggta cgccacagcg tataacgccg tggccatggc tggactcgcc
     9541 gtcctctttt atctcgcggg tgacgccatc gttcggttct tcgatagcga ttcagccgtg
     9601 gtcgatatgg ccgtgcagta cctgcgcgtc gtctcgcctt tctacctagc cttgggcgtc
     9661 ggcatcgtcc ttggcaatgc catcacgggc gcgggggcta cccgactcac cctgaccatc
     9721 gacagcctcg tcgtgctgct cgtccagctt ccgctgagct tgtgggccgt tcaccacgct
     9781 caaacccccg agcgactttg ggctgttctc gcaggcgttc acgtcgcctt cgcttgcgtg
     9841 tacggcttcg tctatcgaaa aggccatttt ttacgtcgcc tcgcatgacg ctgcccattg
     9901 cattgcaccc ttccggtgcc acaatgcccc catggagttt ccttccgata ccaccgttcg
     9961 ctggctcgtt catcgtacgg ccgcccttct cacgcgacag caggatccga atgtcaaacg
    10021 tccggtcgtt ttgcccaacg gcgaattttt tccggatgat ttcgatggcg acgcgggaag
    10081 tgtcaacctg ctgttttggc gtctgcaaga gcatggcaag ctcacggatc tcgatgtcga
    10141 gcttcggttc atcgatgatg gtggagccgc gtccggagca ggatgctgtt cagcggggtc
    10201 gtgtggaacg cagggcaacg cggtggcgcc agctcctttg ccgcctgtcg ttcgtttgtc
    10261 cgacgcctat cgagtgg
//
LOCUS       DAOSOR010000153        10210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6562_length_10210_cov_4.373314, whole genome shotgun sequence.
ACCESSION   DAOSOR010000153 DAOSOR010000000
VERSION     DAOSOR010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 10210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10210
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6562_length_10210_cov_4.373314"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..2279)
                     /locus_tag="PK710_10215"
     CDS             complement(150..2279)
                     /locus_tag="PK710_10215"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT10135.1"
                     /translation="MDRERTLRSAERNAARGRFKQAIDDYNKLLESFPEDARLLLKVG
                     DLYSRLQAYERAVEAYVRAARVYTKEGYAAKAIAVFKQVEKLLSTHVPHLNDYFLFLY
                     PTLANLYQELRLSAEALNTFNIYASLLARAGRIHEAVSILQIVSDRGGASPVTQLRIA
                     EILLEDGYRNDAVHHGIEAANRLVSLHRIDDAVRVLEQMHARTQAAEISRQAAKILLE
                     RGQTNDGMHALVHLQNAFRAEPKNLDTLELLTRVFDSMGQHDRAHEVRKQTVRIAKEQ
                     GAMEIALSTLATLRDYAPHDPDVEKLALSLVPSSPAQHLAAPPSIKQTESTENHVLPI
                     QSDVPFIEETGVLAQIERVGDVANLEQQAREFLDRGDLQNAIHSVNIGLELEPNHQSL
                     RKMLATLVELQSSALAPKPIPMEILQSVAESPADPSADSSPANTDVMAALPLKEIRGF
                     RGGGGVSEALEEVEFFVSRGLFEDALAILQEQIALSPHHPELLQRFKEVEQSMLEADA
                     QPSVLKLKQPIEQPSAFEQPVDVALPSPVAPANADIDSLFLMFKQGVETQVDAQDSRT
                     QYDLGMMFMEMGRLEEARIAFENAAKDPARACVSLSMIASIHQRSGHAEQALQVLQRA
                     LSIECKSRSEEVGVQYELSRLFEAREQFSQAIQHLEQVVHLAPDFRDARTRLDSLRQQ
                     VHRGEVTIADDELERVFDDLIGPSKKA"
     gene            2519..2845
                     /locus_tag="PK710_10220"
     CDS             2519..2845
                     /locus_tag="PK710_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10136.1"
                     /translation="MLFALLPSRLQRLARLARRSFHATTQEAQALRGRWVALDHCHYD
                     EGDSIPSSGRVVDSDSDLGELVSRLRLRGGHRCVIVRCEHGCDWDPQTGVYRSRRATH
                     DLTPAA"
     gene            complement(2916..3227)
                     /locus_tag="PK710_10225"
     CDS             complement(2916..3227)
                     /locus_tag="PK710_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOT10137.1"
                     /translation="MHRSIRWIVLVLIGAFGLPNCVPAPARTVSMRMHGADSDAIVHI
                     DDQYLGTFSIVEARGVALPPGQHRISVEKPGYFPWDRLVDVKEGDPLLRLEVELKKIP
                     D"
     gene            3313..7593
                     /locus_tag="PK710_10230"
     CDS             3313..7593
                     /locus_tag="PK710_10230"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT10138.1"
                     /translation="MPSSHELEFENGTSVESEVVSAAWRIEKILGIGGTATVFLATGL
                     HGQRSALKVMRPEAAQQQGALQRFLREGRLARHIEHAGMVRCLGEGTTSDSRPYLALE
                     YLEGCTLEALVRSGDHLSLLPLLRLFVDVLDLMAHCHSRGIVHRDLKPSNLFVTRTGM
                     VKVLDFGVATAQGVVTDYVREGVAIGTPSFMAPEQAMGDWEHVDARADVFSLGATLYS
                     LLSGKRLHVGKNEAESFTLAATRSAPSLGEVAPELPTEIVSFVDRALQWDKRSRFADA
                     ACMKAQLEGILLGFEEVDTKTTKPWQRQEDIAPKAIVDVPQDALCCSKAFSLLPRALE
                     QWEQGLDHDATLKEAADYLVRVSQAGGSDWRLTVRPWSILLRDETVWSPHWPWLPVIQ
                     QMFDDGIRFMACEPTLAGAERLFRRIFDQVVVESRRADLVAEFWPLSHQGVRIGVAWG
                     LLGTTASRVNEACESQTTVLRLMEASGEDIDRLEFLETWPCVSELLLDDHTRSPWLSP
                     TDDHYDALFSRYLVAMLEEMAKGRVLISRDESILRTLARDLLEEEQTDTLLFASLSLA
                     PSETRLDVLTALWLPEALPRLLKHLNRRVPAEALEDRRLSNLRKLLDIFDTRAASICL
                     RVLPELTHPTLARIVVQYVVSHLPEQMEGIADVLFRLTGETGYRLIKALGEQKHDEVR
                     VVLERLEQTTSPLRIAAKAMLYGIEREEGSVISQLAAPGWPARAVALWMVKEHRLESA
                     EPWLVRTIEDPSFHHRSRQERRLVFEALVALSLQQAEQRAEALVRRHGLIREDAVDDT
                     RCLAVELLGEISSNEAILSTLRGAESPMWWNPRAIRVAARTARQRVEQRLGIDRKKSV
                     AEARSSTLPPEQVPGSSTSGESTKTSPNLSLRHVPLPRNDEAWPNEQVARFFVSLACL
                     VELVQRQADEDSLQLEALTTVDAVRQQAQRSGKLHVTMEAGVPYVDKKPVLLAAWVAQ
                     QIEPLVGALQWLEWLDIRIDPRLTPDDAIAFATAIATESSLESVRYVHVGKSNLQDLS
                     DVIILDPHTSSNELKSTYATILAALWEARSAWAREQIPDLDGMLRVSQMVVDLAMTRR
                     VAITGAKLGGDVGARWLDAALLAVEMVSLLESDPEQLRDVALAGLLLSVIPFDERGAV
                     IKEGTTSWLRAGRLGDPSHLAQKTFSILMGISGIHGVIRRAITLASEAQARRYRRLSV
                     GASSASVWPAVALATSIRYVESLSRQVPGDDALVEMLREESMDTTDEVCVRLLYSALG
                     EVPRGAVVELASREVGVLRTPLRAWSEQVPIVAMAADELSREIPEFSRLEERPLASRS
                     SLRAVLSLWKGEQLPQARPRRGKWGHKKRGDVRESKDRSKRPQAPLVSTLGNDILEDF
                     GLPTLGQPVPPSISDRLHSSAATLAPCGEETLKGVKMTDDLLGAEDDG"
     gene            7800..8924
                     /locus_tag="PK710_10235"
     CDS             7800..8924
                     /locus_tag="PK710_10235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOT10139.1"
                     /translation="MALDMQPRMQGIEDDKVRLSDGIARRAKEPSCIFALLKALNSPD
                     LEMVMQRVVEALPSALTHAELAYAAVTIRGKTCSTVIAERRKLSLRAPIVVSDEEAGV
                     LEIGYVDVFPDGRTPRFESEEHELLVLIANRMGEYLALREAQSQISTYQDHLRCLASE
                     LTLTEERQRRTLALAVHDRIGQGLAVAKLKLESLRHVLPSEHQSRLDDVTVLIKQIVH
                     DTRTLTFEISPPVLYELGLKQAVVWLSEHVKRDFGLPVTVRCDEPLSAIGEGVRVMLF
                     RSIQELLTNVVKHANATRATVHLRNELDQICVVVEDDGIGFDVKGLGHRPSTSGGFGL
                     FSIRERIHHLGGKIEVFSVLGEGSRIQVFVPRGDATEPRA"
     gene            8921..9586
                     /locus_tag="PK710_10240"
     CDS             8921..9586
                     /locus_tag="PK710_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT10140.1"
                     /translation="MRILLADNHKLFCEGLRTLFAGQTGVEIVGVATDGRMAVRLARE
                     LSPDLVIMDIGMPELNGIDATRQIRSEMPRIKVLAVSMHADRQYVAGMLSAGAAGYVG
                     KDSAFSDLMKAVEMVLGGGCYLSPDVVGVVVDDYVQRLAPVPGTGLAKLSEREREVLQ
                     LMAEGYGTIDIGERLHVSRKTVETHRKNIMVKLELHSVADLTKFAVREGLTSLETMRV
                     KRN"
     gene            9838..>10210
                     /locus_tag="PK710_10245"
     CDS             9838..>10210
                     /locus_tag="PK710_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10141.1"
                     /translation="MYRKLDYFYASIKDESGAGFRFLDRLATKQVDLTALTAVPFGPD
                     RAQITIFPTDAASLVAAAKEENIALDGPHTAILVEGDDQVGAFAKVMAKLAAVNINVF
                     SLQGMTDGRGGFRYIVFVRPSD"
BASE COUNT         2155 a         2384 c         3273 g         2398 t
ORIGIN      
        1 aggatatggc ggcggcggcg gtggaggata tggcggccga ggacgcggtg gacgtggtgg
       61 tggaggtgga ggtgggcgtc gtgggccagg tggaggacgt ggcgcacgag gtggaggtgg
      121 tggagagggc caacgctggt aggctcgaac tacgcctttt ttgaagggcc aatcaagtca
      181 tcgaacactc gctcgagttc gtcgtcggca atcgtgactt caccacgatg cacctgttgt
      241 cgaagactat ccaaacgtgt gcgggcgtct cgaaagtcag gtgcgagatg aaccacctgt
      301 tcgaggtgtt ggatagcttg cgaaaactgt tctcgggcct cgaaaagacg actcaattcg
      361 tattgcacgc cgacttcttc cgatcgtgat ttgcattcga tcgaaagggc acgctgcaaa
      421 acctgtaggg cttgttccgc atggcccgag cgttgatgaa tcgacgcgat catggacagg
      481 ctgacgcagg cccgcgcggg atctttcgcc gcattttcaa aggcgattct agcctcttcg
      541 aggcgtccca tttccatgaa catcatacct aaatcgtatt gcgtcctgct gtcttgcgcg
      601 tcgacctgtg tttcgacgcc ctgtttgaac atgaggaaca gcgaatcgat atcggcgttc
      661 gcgggagcga caggagacgg aagcgcgaca tccactggct gttcgaaagc agatggttgt
      721 tcgatgggct gtttgagttt gagtaccgat ggttgggcat cggcctccag catcgattgt
      781 tcgacttctt tgaatcgctg caatagctca ggatgatggg gagacagagc gatttgttct
      841 tgcaggatgg ccaacgcgtc ttcgaataat cccctggaaa cgaaaaactc tacctcttcg
      901 agagcttcgg agacccctcc tccgccgcgg aaccctcgga tttctttgag cggcagagcc
      961 gccatgacat cggtattcgc cggtgatgaa tcggcagacg gatcggctgg agactctgcc
     1021 acggattgca ggatttccat gggtatgggc ttgggtgcca aggcggagga ttgaagttca
     1081 accaacgtag cgagcatctt tcgcagcgat tggtgattcg gttccaactc gagcccgatg
     1141 ttgacactgt gaatggcgtt ttgcagatcg cctcggtcta aaaactcacg ggcttgttgc
     1201 tcgaggttcg ccacgtcgcc aactcgttcg atttgagcca aaacgcctgt ttcctcgatg
     1261 aaaggaacgt cgctctgaat aggcaaaacg tggttttccg tggattccgt ttgcttgatg
     1321 ctcggtgggg cggccaggtg ttgtgcggga gaggatggga caagggaaag ggccagtttt
     1381 tcgacgtcgg ggtcatgcgg ggcgtaatca cgaagcgtcg cgagtgtcga aagagcaatt
     1441 tccatcgccc cttgttcttt ggcgatgcgg acggtttgtt tgcggacctc atgggcgcgg
     1501 tcatgttgtc ccatggaatc gaaaacgcgc gtgagcagtt cgagcgtatc gaggttcttg
     1561 ggttcggcgc gaaatgcgtt ttgaagatga accagagcat gcatcccatc gttggtctgt
     1621 ccccgttcca gcagaatttt ggcggcctga cgtgatattt ccgcggcttg ggtacgcgca
     1681 tgcatctgct ccaacacacg cacagcatcg tcgattcggt gcaaactgac gaggcgattg
     1741 gctgcctcga tcccatggtg gaccgcatcg ttgcggtagc cgtcttcaag caagatttct
     1801 gcaatacgca actgcgtgac gggggatgct ccaccgcgat cggaaacgat ctgaagaatc
     1861 gatacggctt cgtgaatacg gcctgcgcgt gcgagaaggg aggcgtaaat attgaatgta
     1921 ttgagtgctt cggcggacag ccgtagttct tggtacaagt tggcgagcgt gggatatagg
     1981 aacaggaagt agtcgttgag atgggggacg tgggtggaaa gcagtttttc tacttgcttg
     2041 aaaacggcga tggctttggc agcataacct tctttggtgt agacgcgggc agctctgacg
     2101 taggcttcca cggccctttc atacgcttgc aatcggctgt aaaggtctcc gactttgagt
     2161 agcaagcggg catcttcagg gaaggattcg agaagcttgt tgtaatcgtc gatggcctgt
     2221 ttgaaccggc ctcgagcggc gtttctttcg gcggatcgca gggtacgttc acggtccatg
     2281 ggtattcgtt cccttcggtc gatctaccct ccgattgtgc gccgaggtag gacaagggtc
     2341 aaagattagg cgaaatgaag cgagtcattc tgtgcagagg aacgattcac ggccgtggca
     2401 tccggttgga tgtgtggccg ctgtgacgcg gcgatcaggg gcagaggcat gcgaaagccc
     2461 aaatttgcgg cgtagaggcg caaggtgggg cggcatggga tttgaatgac gaaatcccat
     2521 gttgtttgcg ttgcttcctt ctcggcttca gcgtctcgcc aggttggcac gaaggtcgtt
     2581 tcacgcaacc acacaagagg cacaggcact gcgggggcga tgggtcgcgc tcgaccactg
     2641 tcattacgac gaaggcgatt cgattccatc gtcaggacga gtcgttgaca gcgattcgga
     2701 tcttggggag ttggtaagtc gattgcgtct aagaggggga caccgttgcg tgatcgtgcg
     2761 ttgcgaacac ggatgcgatt gggatcctca aacgggcgtt tatcgaagtc gtcgagcgac
     2821 gcacgatctg acgccggcgg cctgaacggc atatggcttc gagatttgct ggaggagggt
     2881 aacggggcgg agtcgaattg gcaacgaatg agcgattagt ccgggatttt tttgagttct
     2941 acttcgagtc gaagcagtgg gtcaccttcc ttgacgtcga cgaggcggtc ccaagggaag
     3001 tatcctggtt tttccacgga tattcgatgc tgtcctggcg gtaaggccac gccgcgggct
     3061 tcgacgatcg aaaacgtgcc aaggtattga tcatcgatat gaacgatggc gtccgaatct
     3121 gcaccgtgca tgcgcatgga taccgttcgg gcgggagctg gaacgcagtt tggcaagcca
     3181 aacgccccga taagaacaag gacaatccat ctgattgaac gatgcatggc aagggagtcc
     3241 tgccaggctc tgggttcgaa tgcaagcgag cgtggacgtc ggcgctggaa ttgcctaccg
     3301 tagctcggcg tcatgccatc ttcgcacgaa ttggaattcg aaaacggcac ctcagtcgaa
     3361 agcgaagtgg tatccgccgc gtggcgtatc gaaaagatat tgggaatcgg aggaacggcc
     3421 acggtttttt tggcgaccgg gttacacggc caacggtctg ccctcaaggt catgcgccct
     3481 gaagcagcgc agcagcaggg ggcgcttcaa cgttttttac gcgaaggacg tctggcgcgg
     3541 catatcgaac atgctggaat ggttcgctgt ttgggtgagg gaacgacatc cgactcgcgt
     3601 ccgtatttgg cgctcgagta tctcgaggga tgcaccctcg aggcgctggt tcgatctggc
     3661 gatcatctct cgctgctgcc attgctaagg ttgtttgtcg atgtgctgga tctcatggcc
     3721 cattgtcata gccgcggaat cgttcatcgg gacttgaagc catccaacct gttcgtaacg
     3781 aggacaggaa tggtgaaggt gcttgatttc ggggtggcga cagcacaggg agtcgtgacg
     3841 gattacgtac gggagggagt tgccattggg acgccttcgt tcatggcacc cgaacaagcg
     3901 atgggggatt gggaacacgt ggatgcacgt gccgacgtct tttccctggg agcgacgctc
     3961 tattcgttgc tgtcgggcaa acgattgcac gtcggaaaaa acgaagcgga atcgtttacg
     4021 ttagcggcca cgcgttcagc tccttctttg ggcgaagttg cgcccgaatt acccacggaa
     4081 atcgtaagtt tcgtcgaccg cgcgttgcaa tgggacaagc gttctcgctt cgccgatgcg
     4141 gcttgtatga aagcgcaact cgaaggcatc cttctggggt tcgaagaggt tgacacgaag
     4201 acgacgaaac catggcaacg acaagaggat attgctccga aagccatcgt cgatgttccg
     4261 caagacgccc tttgttgttc aaaggcgttt tccttgttac cacgagcatt ggagcaatgg
     4321 gagcaggggc tcgaccatga cgccacattg aaggaagcgg cggactacct tgtgcgcgta
     4381 tcacaggcag gtggttcgga ttggcgtcta acggtacgac cgtggtcgat tctacttcgt
     4441 gatgaaaccg tgtggtcacc tcattggccg tggttgcctg tcattcagca gatgttcgac
     4501 gatgggattc gtttcatggc gtgcgagcca acgctcgctg gggctgagag gctgttccga
     4561 aggatatttg atcaagtcgt agtcgagtcc cggcgtgccg atcttgtggc tgagttttgg
     4621 cctttgtctc atcaaggcgt tcgaattggc gtggcttggg gattgcttgg aaccacggca
     4681 tcccgtgtga acgaggcttg cgagagtcaa actaccgtgc ttcgcctgat ggaagcatcg
     4741 ggagaggata tcgatcgtct cgagttcttg gagacttggc cgtgtgtatc agaattgttg
     4801 ttggatgatc acacgcgctc gccatggtta tcgcccactg acgatcacta cgatgccttg
     4861 ttttctcgct atctcgtcgc gatgttggaa gagatggcga agggtcgagt tcttatttcc
     4921 cgcgatgaat ccattcttcg aaccctggca cgggatctgt tggaggaaga gcaaactgat
     4981 acgttgttgt ttgcttcatt gtcgttggct ccaagcgaaa ccagattgga tgtgctgacc
     5041 gctctgtggt tacccgaggc gcttccacgg ttgttgaagc atctcaaccg tcgggttcct
     5101 gcggaggctc tcgaagaccg acgactatct aacctacgga aattgctaga tatatttgat
     5161 actcgcgcag cttctatctg tcttcgggtg ctgcccgaac ttacccatcc aaccttggca
     5221 cgaatcgttg tgcagtacgt ggtgtctcat ctgccggaac agatggaagg gattgccgac
     5281 gtgctatttc gtctgacagg agagacgggg tatcggttga tcaaggcgtt gggggaacag
     5341 aagcatgacg aagtgcgagt cgtgttggag aggttggaac aaaccacttc gcctcttcgc
     5401 attgcggcta aggcaatgtt gtatgggatc gagagagaag aggggtcggt gatatcgcaa
     5461 ctggcagctc caggatggcc tgcgcgtgcg gtagctctgt ggatggtgaa ggagcatcgg
     5521 ttggagagcg cggaaccatg gcttgttcgg accatcgaag atccctcgtt tcaccaccgt
     5581 tctcgacaag agcgtcgcct cgttttcgaa gcactggttg cgctttcttt gcagcaagcg
     5641 gaacaacggg ccgaggcgtt ggtgcgtcga catggtttga ttcgagagga tgccgtggac
     5701 gacacgcggt gtttggctgt cgagctattg ggagagattt cgtcgaatga agccatccta
     5761 tcgacgcttc gcggtgcgga atctccgatg tggtggaatc cgcgggccat acgagttgct
     5821 gctcggactg cgcgtcaacg agtcgagcag cgtttgggta tcgaccgaaa gaagtccgtg
     5881 gctgaagctc gcagcagtac actgccaccg gagcaggtgc cgggttcttc aacctcaggc
     5941 gagtccacga aaacttcgcc caatctatcg ttgcgacacg taccgttacc caggaacgat
     6001 gaagcttggc cgaacgaaca agtggcgcgg tttttcgtat cgctggcgtg tcttgtcgag
     6061 ttggtacaac gtcaggccga cgaggactct ttgcaactcg aggcgctgac cacggtggac
     6121 gcggttcgac agcaagcaca aaggagcggc aagctgcacg tgacgatgga agcgggtgtt
     6181 ccttatgtgg acaagaaacc tgtgttgctg gcggcgtggg ttgctcaaca aatcgagcct
     6241 ctcgtcggag cgttacaatg gctcgaatgg cttgacattc gaatcgatcc tcgtctgaca
     6301 cccgacgatg ccattgcttt tgccacggcg attgctaccg aaagttcgtt ggaatccgtt
     6361 cgttacgttc acgtggggaa atcgaatctg caagatttat ccgacgtcat catccttgat
     6421 cctcatacgt cctcgaacga attgaaatcg acgtatgcga cgatattggc tgcattgtgg
     6481 gaggctcgtt ccgcgtgggc tcgggaacag ataccggatt tggatggtat gcttcgtgtt
     6541 tcgcaaatgg tggtggatct tgcgatgact cgtcgtgttg cgatcacggg agcgaagctg
     6601 ggtggagatg tgggggcgcg gtggctggac gcggcattgt tggcggtgga gatggtatcg
     6661 ctattggaaa gcgatcctga acagcttcgt gacgtagctt tggcggggtt gctcctctcg
     6721 gtcattccct tcgacgagcg cggggccgtc atcaaagagg ggaccacctc atggcttcga
     6781 gccgggagac tcggagatcc aagccatttg gcacaaaaaa cgttttcgat actgatgggg
     6841 atttcgggga tacatggtgt gattcgacga gcaataacgt tggcatcgga ggcgcaagct
     6901 cgtcgttatc gacgtttgtc ggtgggtgcc tcatcggcga gtgtttggcc tgcggtcgct
     6961 ctagcgacgt cgattcgata cgtagagtca ttgtcgagac aggtgccggg agatgatgcg
     7021 ttggtggaga tgttgcgcga agaaagtatg gacacgaccg acgaggtatg cgttcgattg
     7081 ttgtattcgg cgcttgggga agtgcctcga ggggcggtgg tggaattagc gtcgcgagag
     7141 gtgggcgtgt tgcggacgcc gcttcgggca tggagtgagc aagtgccgat tgtggccatg
     7201 gcagcggatg aattgagcag ggaaataccc gagttttctc gtctggaaga gcggcccttg
     7261 gcaagtagga gttcgcttcg tgcggtattg tctctttgga aaggcgagca attgcctcag
     7321 gcacgaccac gaagggggaa atggggtcac aagaaaaggg gggacgtgcg agagtccaaa
     7381 gatagatcca aacggcccca agcccccctg gtatcgactt tgggtaacga tattttggag
     7441 gatttcgggc tacctaccct tgggcaaccc gtgccgccat cgatttcgga tcgccttcat
     7501 tcctctgctg cgactcttgc tccctgtggg gaagagacgc tgaaaggcgt caagatgacc
     7561 gatgatctgc tgggtgcgga agacgatggt tgagtcgacg gggtggtgga atcatcgtgg
     7621 agacggcatc tcgaaagcca cggaaaggtg tggtttgcca ggaatcgacg gctaggctag
     7681 gctcaggggg cttcgccatt gtcgctagcg tctttgttcc tcgacgcggt atcaatggcg
     7741 aaggacgacg ctgctcgcat cgttcaacac gagttggtga ggatgggaaa gcgatgacca
     7801 tggctttgga tatgcaacct cgcatgcagg gcatcgagga cgacaaggtc cggttgtctg
     7861 atggaattgc gcggcgagcc aaggagccaa gctgcatctt tgcactattg aaagcattga
     7921 actcaccgga tctcgaaatg gtgatgcagc gtgtggttga ggcattgcct tcggcgttga
     7981 cgcacgctga actggcgtat gccgccgtga cgattcgggg taagacatgt tccacggtga
     8041 ttgctgaacg aaggaaattg tcgttacgag caccgatcgt cgtgagcgat gaggaagcgg
     8101 gtgtgttgga gatcggatac gtggacgttt ttcctgatgg aagaacgcct cgattcgaat
     8161 cggaggagca tgaattactg gtgctgatag ccaaccgcat gggtgagtat ctggccttgc
     8221 gagaagcaca atcgcagatt tcgacctacc aggatcatct ccggtgtttg gcttcggaac
     8281 tgacgttgac ggaagagcga caacggcgga cccttgcgtt ggcggtacac gaccgcattg
     8341 ggcaagggct cgcagtagcg aaactcaaac tggagtcgct caggcatgtt cttccttcgg
     8401 agcatcaatc tcggctggat gatgtcactg ttctcatcaa gcaaatcgtc catgacacgc
     8461 ggacactcac gttcgaaatt agtccgccgg ttctttatga gcttggattg aagcaagcag
     8521 tcgtctggct tagcgagcat gtaaaacggg attttggact tccggtaacg gtgcgttgcg
     8581 acgagccgct gagcgcgatc ggcgaaggtg ttcgtgtgat gctgtttcgt tctattcaag
     8641 agttgctcac gaacgtggtg aagcatgcga acgcaacgcg ggccacggtc cacttgcgga
     8701 acgaactcga ccagatatgc gtcgtggtgg aggacgatgg aattggattc gacgtcaagg
     8761 gattgggaca tcgtccatcg acctcggggg gcttcggatt gttcagcatc cgggagcgaa
     8821 ttcatcattt gggaggcaaa attgaggttt tttccgttct gggggaaggc agtcggattc
     8881 aggttttcgt tcctcggggt gatgccacgg agccgcgagc atgaggatac tgctagcaga
     8941 caatcacaaa ttgttttgtg agggattgcg gacgctattc gctggacaaa cgggggtcga
     9001 gattgtgggg gtagcgacgg atggtcggat ggcggttcgg cttgcgcgtg aattatcacc
     9061 ggatttggtg atcatggata ttggcatgcc cgagttgaac ggcattgatg ccacgcggca
     9121 gatacgttcc gagatgcctc gaatcaaggt gcttgccgta tcgatgcatg cggatcggca
     9181 gtatgtggct gggatgctat cggcgggagc cgctgggtac gtgggcaaag acagtgcgtt
     9241 ttctgatctc atgaaggcgg tggagatggt attggggggc gggtgttact tgagtcccga
     9301 tgtcgttggt gtggttgtcg atgattatgt gcaacggctt gcgccggtgc ctgggaccgg
     9361 acttgcgaaa ttatccgagc gagagcgcga agttttgcaa ctcatggccg agggctatgg
     9421 aaccattgac attggggaac gtttgcatgt cagccgaaag acggtggaga cgcatcgaaa
     9481 gaacatcatg gtgaagctgg aattgcatag tgtggctgat ctcacgaagt ttgcggttcg
     9541 tgagggattg acatcgttgg agacgatgcg ggtcaaacgc aattgactaa gagatttcct
     9601 tagatatatt cccattttgg cagtacgggt ctgctcaaag acgaaagaaa aatagggata
     9661 atcccaatac ctacggacaa taagactgcc tacagtcccg aaaacgcggc gttcgcgtca
     9721 gactcgttgg ctgttcgcat catgggtgac agggacggtc atgccgcggc aatgattggt
     9781 caaaaccggg cgcaactttc aaaccggaaa gacacgagcg accgagggag gatgaatatg
     9841 tatcgcaaac tcgactattt ttacgcaagc atcaaggacg aatctggggc aggttttcgg
     9901 tttcttgatc ggctcgctac caagcaggtg gatttgacgg cgttgacggc ggtgccgttt
     9961 ggcccggatc gagcgcaaat cacgatcttt cccaccgatg cggcgagttt ggtggcggcg
    10021 gccaaggagg agaatatcgc gctcgatggt ccgcatacgg cgattctcgt ggagggagat
    10081 gaccaagttg gtgctttcgc caaggtgatg gcgaagcttg cggcagtcaa tatcaacgtt
    10141 ttttcccttc agggaatgac ggatggccgt gggggatttc ggtacatcgt gttcgtgcga
    10201 ccgagtgaca
//
LOCUS       DAOSOR010000154        10210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6564_length_10210_cov_3.924865, whole genome shotgun sequence.
ACCESSION   DAOSOR010000154 DAOSOR010000000
VERSION     DAOSOR010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 10210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10210
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6564_length_10210_cov_3.924865"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1643
                     /locus_tag="PK710_10250"
     CDS             <1..1643
                     /locus_tag="PK710_10250"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HOT10142.1"
                     /translation="GRQPDFILPRPYVAIVLEQVFHALTAAHRAGVIHRDIKPENVFL
                     ARTRRVGAPFVVKVLDFGIAKLLSESRPASQTMSVGTPGWLAPEQLEHGITSPAADVW
                     ALGLLCYFVFTGARFWVRENEENASVQMLLFEMVMAPIPTASARAAEQQRAHLLPPGF
                     DAWFAKCLVRDPTQRFADAAEAWEALEPILQSAIPAQNPNNLAFQAHPPADLGQANPH
                     SALRIGPNVTNEGLATAHPGLTGTGPLVRSSKTPVAIIGAALGAMVLLAGIGIAWWAL
                     TNTNTPSPSASSVYPSSSSAAASALPAVASSVPSTLVAESILIPETGFTMDGIPIRVG
                     PVEVDRLEVRVAAWQECVEAGACDSNVASVNVRGAESWSVLCNWPRRIERSEHPMNCV
                     SRKQAEAYCAWKGKRLPTEAEWYLAAYASGGDVRTYPWGNEAPRDGVINLCGAECAAM
                     LRASKLLQNKEPLADATDDYADTGPVTAFPKDLSPEGLLGLGGNVAEWTSTDHEGGKV
                     RCRGGSWLSNKWSDASKDVVFAIDPKERRANVGFRCVK"
     gene            1897..2634
                     /locus_tag="PK710_10255"
     CDS             1897..2634
                     /locus_tag="PK710_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10143.1"
                     /translation="MVKTRWAGWVSGVVLATLSMVANGQELEPVKPTPTTPPPVQRPL
                     QPPTQRQPDAQLPPTQPSTPSEPCPSGTVWRDDQCVTETAPAPDPPPAPVPPAPCGEN
                     MAWNGKECVNNAPPAPTPPSNDTSEASSGSGGGWRTAAIIGGGVLLGAGAVTGILAFS
                     KKSKWSEACEDKHCYPSAQDDYDSGKLMANLSTGTFIVGAAVLTAGLFLIPGASSPAD
                     ERPVAGKPYVNIAVGPHGAGIGSRWVF"
     gene            3058..4146
                     /locus_tag="PK710_10260"
     CDS             3058..4146
                     /locus_tag="PK710_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10144.1"
                     /translation="MMKKTCLIALATAALLLGGCCKDKTSETRTEAAADGKGLGDPSN
                     DPAIVAIAKPVLECKWTSYGFDSKCAAFESWKKDESLKDGKGDKTLVSFLEDADTKVQ
                     WLGADTLSRVGQLYRKDQVLATKVVDATDKVADSLLASTHARATGNIDLSVTGLGPRV
                     MKMLQEHSFPGLRQELAGSVLFRNSNVPGMYDLFVKLARSDKDPKVRKAAAAAFWTGT
                     PTGKNEEVCKLWLELADDADSDLAGHSAYHCSFTSSGGGCVGQWDALLSLIERKAKAG
                     QVKSSFMAVSLKYFYGQQKATAAQKKRALEIAKTLVANTANDGSSRSSALEFVGKEDP
                     AGKAFAAKYENDKEFFVKSTAKRIREQK"
     gene            complement(4149..4919)
                     /locus_tag="PK710_10265"
     CDS             complement(4149..4919)
                     /locus_tag="PK710_10265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HOT10145.1"
                     /translation="MRIESVERVIERMESAVQAGASVVATDADGTLWSGDVGVDIFET
                     LIAERGIREQAEEALRREATEASIDSTGSVHEVAAKLEAAVATGQYSEQRCYEMMAWV
                     FAGWAVEQAREFARQVRDRKGLSLRIHGEMRAIVEWISKRGLELWVVSASPSFMVQAG
                     VELLGIGADRVIGTTPAQEQGRWLARLESPIPYGPGKVQELKKKTKNQIVAASFGDEV
                     FDVEMLKEAKVAVAIRPKARLRGRASEITGLVELERRL"
     gene            complement(4930..5736)
                     /gene="gloB"
                     /locus_tag="PK710_10270"
     CDS             complement(4930..5736)
                     /gene="gloB"
                     /locus_tag="PK710_10270"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HOT10146.1"
                     /translation="MVSEGFVSGGADDMKVQIVACSFDNYAYLVHREGSSKALVIDPS
                     EGIPVLREAEHLGLELVAVLCTHHHHDHVGGIAEVREAFPTIEVWAHEHDRHRIAGVT
                     HTVQHAQTIVVGGMSFSALHVPGHTLGSVAWICSNLLWTGDTLFVGGCGRVFEGTPTM
                     MQASLQRLASLPQDTQVYCGHEYTLSNLRFARLVESGHDPIQKHLRQAETLRAENKPT
                     VPSTIGQELLINPFLRWCEPEVIAFALRNGARDKTPTSVFAAVREAKNKT"
     gene            complement(5730..6677)
                     /locus_tag="PK710_10275"
     CDS             complement(5730..6677)
                     /locus_tag="PK710_10275"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOT10147.1"
                     /translation="MKFPPLTPQALWRAIKPTFSRNLGLKAFSLACAITLYAFVHGAQ
                     DAQRIMAVDLVVLLPPASQNRTLTTDLPTSIRVSLHGPRSIVGDLRSSDLGNMQLDLR
                     MGRTGTIPLEPGMLRVPGGVTIDSIDPSTLDISWDDVVERPIQVQVPVTGAPAEGFVI
                     KGRASAIPDTLLARGPSHVVGNLQVARVEPFDISGLSDGTHKRTLVVERPPPRVTYDV
                     THATATVEIIRKLRERVFSKVHVQIVGVARATALPSQVDVRVLGAPDFVSDLRSEQIV
                     PRVTVREADVNKKTAALPIELELDGVQVSFVPKTVVVKW"
     gene            complement(6674..7786)
                     /gene="cdaA"
                     /locus_tag="PK710_10280"
     CDS             complement(6674..7786)
                     /gene="cdaA"
                     /locus_tag="PK710_10280"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOT10148.1"
                     /translation="MIAEWLRVWQSMSLLRLAGEVLDVLVVTVLIYRGLLVLKGTRAV
                     QMGFGIVFIFITYVAARWLGLVTLENLFSYLLSSIILIIVVVFQNDIRRALMRLGSNP
                     LFGGWGRALEARVVDEVVAATTELARHRTGALITFEQDATLDEFVVGQGTMIDAAVTK
                     ELLVTMFIPESMNKLHDGAVVIRNLRIARAGVFFPMPEAKNIDAAYGSRHRAAIGITE
                     ETDAVVIVVSEERGTISFCFNGNIVTDLDGEVLRKTLLRIFGRAPKAPMVMDKKKSKG
                     PDSVSRVAIATPPPPRVSTVPRSSPIATPIKIVPVSDGPKSTREISTPMVSDGPKSTR
                     EISTSMASDGPKSTREISTPMASASATPTSKPGDTA"
     gene            complement(7783..8658)
                     /gene="folP"
                     /locus_tag="PK710_10285"
     CDS             complement(7783..8658)
                     /gene="folP"
                     /locus_tag="PK710_10285"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOT10149.1"
                     /translation="MSALRKMLDRRNDNDDPLWMGVLNITPDSFSDGGEYFDRDAALR
                     RVDELVAEGAQLIDIGGESTRPRSAPVPAEEQIRRTTDAIVHAVSRGVIVSIDTTDAQ
                     VAEQALRLGASVVNDVSCLRDGDALARVAARQGAALIIMHAREPMSKMQGFSVCDDDA
                     YQDVIQDVRNEWQQAAKRAMQAGLDAQDVFFDPGLGFNKNARHSIELVARLHEFRSLG
                     HGIVVGPSRKSFLAARVQSTPNRRIGGTIAACLACAARGASVLRIHDVLDVRQAFAVA
                     RDIGQDGVRDWRGSP"
     gene            8815..>10210
                     /locus_tag="PK710_10290"
     CDS             8815..>10210
                     /locus_tag="PK710_10290"
                     /inference="COORDINATES: protein motif:HMM:NF019318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOT10150.1"
                     /translation="MLRTRAQAGSGAILVLSLVFAIAFSAICSFDLVVSAWTPSFNAP
                     AGIAIRVPFGARFVEKGDAGSAQVVYERARMAVAPGTIIDQSLASHREALAYELGRRP
                     PSFASLGALFLIYTALSLLLTTYIVRLGQNRLRLLRSQLGVFILILLMMVATKAILLF
                     TNLPEYWSPIAAVPLWIALAFDRRSALLINIVTAFLAASLLRFDMMFLAIALVRGMTV
                     ILLLMNRRKPVSLLVTSLMGGIASALLYIALSTVLEGNFDLIRDLGRLEGSYILSCIG
                     GGALAGLLATVLRDPVERLLGHVPRDKLLDLTDIEHPLLRKLASEAPGTWEHSRAMAN
                     LAEGAASAIGADALLTRVGAYYHDLGKTVQPTFFVENQGEGPSPHDDLPPEVSADAIM
                     AHVVLGTKILREGGIPEPVVEFVYTHHGTQLVEYFWHKYLQQNPEPALSKDHFRYPGM
                     KPQTKETAILMLVDS"
BASE COUNT         2182 a         3160 c         2673 g         2195 t
ORIGIN      
        1 tcggtcgtca accggatttc atcttgccgc gcccttacgt ggccatcgtg ctcgaacaag
       61 tctttcatgc cctcaccgcg gcccatcggg cgggcgtcat tcaccgcgat atcaagccgg
      121 aaaatgtgtt tttggcgcga acacgcaggg ttggagcccc ctttgtcgtc aaagtcctcg
      181 acttcggcat cgccaaactg ctgtcggagt ctcgtcccgc atcgcaaacc atgagcgtgg
      241 gcacgcctgg atggttggct cccgaacaac tcgaacatgg gatcacctcc cccgctgccg
      301 atgtctgggc gcttggattg ctctgctact tcgtgtttac cggggctcgc ttttgggtgc
      361 gcgagaacga agaaaacgcc tcggtgcaaa tgctgctttt cgaaatggtc atggcaccca
      421 tccccacggc ttccgcgcgg gcggcagaac aacaaagagc acatcttctt cccccaggat
      481 tcgatgcatg gtttgccaag tgtttggttc gtgacccaac ccaacgattt gcagatgccg
      541 ccgaggcatg ggaagccctc gaacctatcc tgcaaagtgc tattcccgcg caaaacccta
      601 ataatttagc ctttcaggca catccgcccg cggacctcgg acaggcaaac ccccattccg
      661 ccctacgcat cggacccaac gtaaccaatg aagggctggc cacagcccat cctggtctga
      721 ctggcactgg tcctcttgtt cggtcaagca aaacgcccgt cgccatcatc ggtgccgccc
      781 tcggtgccat ggtgctattg gctggaatcg gcatcgcttg gtgggccctt accaacacca
      841 ataccccttc gccaagtgct tcctcggtgt acccctcctc ctcgtccgcc gccgcctccg
      901 cgttgccggc tgtcgcctct tccgttccct ccacgctcgt tgccgaatcc atcctcattc
      961 cagagacagg cttcacgatg gatggtatcc ctattcgcgt cggtcccgtc gaagtagacc
     1021 gcctcgaagt ccgtgtcgcc gcctggcaag aatgtgtcga agcgggtgct tgtgattcca
     1081 acgtggcttc tgtcaacgtc cgcggcgccg agtcttggtc cgtgctctgc aattggccac
     1141 gacgaatcga acgctcggaa catccgatga attgcgtgtc gcgaaaacag gcggaagctt
     1201 attgcgcctg gaaaggcaaa cgtttgccga cggaagccga atggtatttg gctgcctacg
     1261 cttcgggcgg cgacgtgcga acatatcctt ggggaaacga agcccctcgt gatggggtca
     1321 tcaatctttg tggcgccgaa tgcgccgcca tgctgcgagc ttcgaaactg ctgcaaaata
     1381 aagaacccct cgccgatgcc acggatgact acgccgatac cggtcccgtc acggctttcc
     1441 caaaggacct ttcgccagag gggcttctcg gattgggcgg aaatgtcgca gaatggacct
     1501 ccaccgacca cgagggcgga aaagtgcgat gcagaggggg aagctggctc tccaacaaat
     1561 ggagcgatgc ctccaaagac gtcgttttcg ccatcgatcc caaggaacga cgggccaacg
     1621 tgggatttcg atgtgtgaaa taggaggata cacgcatcgg gggaagcaaa aaaagtgttg
     1681 catccaacgt gctctagaaa tatgaaaata ttattataaa tcacctggtt gccgttgttt
     1741 tggccatggc gagattgttt ttgccggtca ctcggcccgt ggactcgtgc ttgccgatta
     1801 agtttttcgt gcgatggtta tcgcattccg atacaatccc tcgtgggaat cgttcggtaa
     1861 gatggtgcat ggattgagct ttcaaggaga agatggatgg tgaagacgcg ttgggcgggt
     1921 tgggtgagcg gagtcgtact cgcgacgttg tcgatggtgg cgaacggcca ggagttggaa
     1981 ccggtcaaac caactcccac gacgcctcct ccggtccagc gcccccttca acctccaacg
     2041 caacggcagc cggatgcgca gcttccgcca acgcaacctt cgaccccgtc tgaaccttgc
     2101 cccagcggaa cggtttggcg cgacgatcaa tgcgtaacgg aaacagctcc tgctcccgac
     2161 cccccccccg cacccgtacc accggcccct tgcggggaaa atatggcctg gaatggcaaa
     2221 gaatgcgtga ataacgcgcc tcccgctcca actcctccct cgaacgatac atccgaagcc
     2281 tcctccggct cgggaggtgg ttggcgaacg gctgccatca tcggcggtgg tgtattgctc
     2341 ggggcagggg cggttaccgg tattctggcc ttctccaaga aaagcaagtg gagcgaagcc
     2401 tgcgaagaca aacactgcta tccatccgcg caagacgatt acgattcggg aaagctcatg
     2461 gccaatctgt ccacgggcac tttcatcgtc ggggcagcgg ttcttacggc aggcctgttt
     2521 ttgattcccg gcgcatcctc ccctgccgat gaacgtccgg tcgcgggcaa accctatgtc
     2581 aatatcgccg tcggtccgca cggtgcgggc attggctcgc gctgggtgtt ctgacttcgc
     2641 ctcaatacca tcctagaccc accagaaaaa cgcgccgtac cgcgtacgaa gctgaggtaa
     2701 actttgtaaa tacaatgaat tagcgacaat tgcttgaacc gctgccgtcc tcgaagtcca
     2761 tcgattcttt tgcgataggg ggcctccacg tcgagcctga cagcgtcctt ggttgcctgt
     2821 tgcaccgccc aatcggtatt ttcagctttt tccccgaagc catgggctgt cctcccggca
     2881 tggattcgtg aatgactcgc tggtactgag cttttcaacg ttcaaaacag gaattcgtgc
     2941 gtacaattcg aacgggaatg cttgggaatg accacgaaga aatgacgtag aaaagctcaa
     3001 attcttgatc tatcgttcaa gacgaattac gccgtttcta cgctacgagg agacgatatg
     3061 atgaaaaaga cttgcctcat cgccttggct accgcagcct tgctgttggg agggtgctgc
     3121 aaagacaaga catcggaaac gcgcaccgaa gcggcagccg atggaaaagg gcttggagat
     3181 ccaagcaatg acccggcaat cgtcgctatc gccaaaccgg ttttggaatg caaatggacc
     3241 agctacggtt ttgactccaa atgtgctgcg tttgagtcct ggaagaaaga cgaaagtcta
     3301 aaagacggca aaggcgataa aacgctggtc agttttcttg aagatgcgga taccaaggtg
     3361 cagtggcttg gggcggacac cctgagccgg gtcggacagc tttatcgaaa agaccaggtt
     3421 cttgccacca aggtcgtcga cgctaccgac aaggttgccg attcgttgct ggcctctaca
     3481 cacgcccggg caacaggcaa tatcgacctg agcgtgacgg gcctggggcc ccgcgtcatg
     3541 aaaatgcttc aagaacactc ctttcccggt ctacgccaag aactcgcggg tagcgtgctt
     3601 tttcgaaata gcaacgtgcc cggcatgtat gatcttttcg tgaaattggc tcgttccgac
     3661 aaggatccca aggttcgaaa agcggccgcc gctgccttct ggaccggtac cccgacgggg
     3721 aaaaacgaag aagtctgcaa actttggctc gaactcgccg atgatgccga cagcgacctc
     3781 gccggtcatt ccgcttacca ttgctcattc acctcttctg ggggggggtg tgtgggacag
     3841 tgggatgcct tgctctccct catcgagcga aaagccaaag ctggacaggt caagtccagc
     3901 ttcatggcgg tctccctcaa gtatttctac ggtcagcaaa aagccaccgc ggcacaaaag
     3961 aaacgagcgt tagaaatcgc caaaacgctc gttgccaata ccgccaacga tggttcctct
     4021 cgcagttccg ccctcgaatt cgtcggtaaa gaagacccag cgggcaaggc ctttgccgcc
     4081 aaatacgaaa acgacaaaga gttcttcgta aaaagcaccg ccaaacgcat tcgagaacaa
     4141 aaataacgtc agagcctacg ctcgagttcc accagccccg tgatctcgga agcccggcct
     4201 cgcaacctcg ccttcggacg aatggccacg gcgaccttcg cttctttcag catctccaca
     4261 tcaaagacct catccccaaa agatgctgca actatttgat tttttgtctt ttttttcaat
     4321 tcctggactt tgccggggcc gtagggaatc ggcgactcca accgcgccag ccatcgtcct
     4381 tgctcctgcg ctggtgtcgt tccgatcacc ctatccgcgc caatacccag caactccacg
     4441 cctgcttgca ccatgaacga aggcgaagcg gagacaaccc acaattcgag tcctcgcttg
     4501 gaaatccatt ccacgatcgc tcgcatctcc ccgtggatcc gtagcgatag cccttttcgg
     4561 tcccgcacct gccgcgcgaa ctcccttgct tgctcgacag cccatcccgc aaacacccag
     4621 gccatcattt cataacaccg ttgctccgaa tactgtcccg tggctaccgc cgcctcgagc
     4681 ttcgccgcca cctcatggac tgaccctgtc gagtcgatcg aggcctccgt ggcttcgcgc
     4741 ctcaatgcct cctcggcttg ttcccggatt ccccgttcgg caatcagcgt ctcgaaaata
     4801 tcgactccca catccccaga ccagagcgtg ccatccgcat ccgtcgccac caccgaagcc
     4861 cccgcctgca ccgcggattc catccgttcg atcacgcgct cgaccgattc gattcgcatg
     4921 cttgcacctc tacgtcttgt ttttggcctc gcgcacggca gcaaacacac tcgtgggcgt
     4981 tttgtcccgt gctccgttgc gtaaggcaaa tgctatgact tccggttcgc accatcgtag
     5041 aaatggattg atgagcaact cttgcccgat cgtcgatgga accgttggct tgttttccgc
     5101 tcgcaacgtc tctgcctgcc gtaggtgttt ttgaatgggg tcatggccgg actccacgag
     5161 ccttgcgaat cgaagattcg atagcgtgta ttcatgtccg caatacacct gggtgtcttg
     5221 cggcaacgac gccaaccgct gcaacgaagc ttgcatcatc gttggagtac cttcgaagac
     5281 acgaccgcag ccaccgacga aaagtgtgtc tcctgtccac agcagattcg aacagatcca
     5341 tgccaccgac cccagcgtat ggcccggcac gtgaagcgcg gaaaaagaca tgcccccaac
     5401 gacaatcgtt tgcgcatgct gaaccgtatg agtcacaccg gctatccgat gtcgatcatg
     5461 ctcatgggcc catacctcga tggtgggaaa tgcttcacgc acttcagcga ttccccccac
     5521 gtgatcgtgg tgatgatgag tgcaaagtac ggcaaccagc tcgagcccca ggtgttccgc
     5581 ttcacgcaat accggtatcc cctccgaagg gtcgatgacc aatgccttcg aactaccctc
     5641 acgatgaacg agataggcgt agttgtcgaa cgagcaagca acgatctgca ccttcatgtc
     5701 atcggcccca ccggaaacga aaccttcgct taccacttca cgacgacggt tttaggaacg
     5761 aaggatacct gcacgccatc gagttcgagt tcgatgggta atgcggcggt cttcttgttg
     5821 acgtcggcct cgcggaccgt caccctcggg acgatctgct ccgatcgaag atccgagacg
     5881 aagtccggtg cgccaagcac gcggacatca acctgagagg gaagggccgt tgcacgagcc
     5941 acgccgacaa tctgtacgtg aaccttgctg aatactcgtt cacgaagctt ccgaatgatt
     6001 tccacggtcg ccgtggcgtg agtgacatcg tatgtcactc gtggaggagg tcgttcgacc
     6061 acgagcgtac gcttgtgggt tccatcggaa agtcctgaaa tatcaaatgg ttccacgcga
     6121 gccacctgca ggtttcccac cacgtggcta ggtccgcgag ccaacaacgt atcggggatg
     6181 gccgatgccc ttcccttgat cacaaatcct tccgcgggtg ctcccgtcac ggggacctgc
     6241 acctgaattg gcctttccac gacatcatcc caagagatgt cgagcgtgga agggtcgatg
     6301 gaatcgatgg tcacaccgcc aggaacacga agcatgcccg gttcgagcgg aatggttccc
     6361 gttcgtccca tccgcaaatc aagctgcata ttgccgagat cggaggatcg caggtcgccg
     6421 acgatcgatc ggggaccgtg caagctgact cgaatcgatg tgggcagatc cgtggtcagg
     6481 gtgcggtttt gcgaagcggg gggcaagagc acgaccaagt cgacggccat gatgcgttgt
     6541 gcatcctggg caccgtgaac gaatgcgtag agtgtgatgg cgcatgccag gctgaacgct
     6601 ttcaacccga ggttgcgaga aaacgttggt ttgatggcac gccacaaagc ttggggtgtt
     6661 aaaggaggga atttcatgct gtatcacccg gtttgctcgt gggagttgcc gacgcggatg
     6721 ccatcggggt ggagatttcc cgggtagatt tcggcccgtc ggatgccatc gaggtggaga
     6781 tttcccgggt agatttcggc ccgtcggata ccatcggggt ggagatttct cgggtagatt
     6841 tcggcccgtc ggataccgga acgatcttga tcggcgtggc tatcggtgaa gaacgaggca
     6901 cggtggatac acgaggcggc ggaggtgtgg cgatcgccac ccgactcacc gaatccggcc
     6961 ctttgctttt cttcttatcc atcaccatcg gagctttcgg cgcacgacca aaaatgcgca
     7021 acagcgtttt gcgaagcact tcaccatcga gatccgtcac gatgtttcca ttgaaacaaa
     7081 agcttatggt tccacgttct tcgctcacca cgatcaccac cgcatccgtt tcctccgtga
     7141 ttccaatcgc tgcccggtga cgagacccat aggctgcatc gatatttttt gcttctggca
     7201 tgggaaaaaa cacgccagcc ctcgcaatgc gaaggttccg aatgaccacc gctccgtcgt
     7261 gcaacttgtt catcgactcg gggatgaaca tcgttaccaa cagctccttg gtcaccgccg
     7321 catcaatcat cgttccttgg cccaccacga actcatccaa ggtcgcgtct tgctcgaacg
     7381 tgatcaaagc tcccgtccga tgccgtgcca gctccgtggt cgccgccacg acctcgtcga
     7441 ccacacgtgc ctcgagcgct cgaccccatc cgccaaacaa cggattggag ccgagacgca
     7501 tcaaggcacg tcgaatatcg ttttggaata ccaccacgat gatcaaaatg atcgacgata
     7561 gaagatagga aaacaggttt tccagcgtga cgagccctag ccatcgcgcc gccacgtagg
     7621 tgatgaaaat gaatacgatc ccaaatccca tctggaccgc gcgcgtgcct ttgagcacca
     7681 gcagcccgcg ataaatcaat accgtcacga ccaaaacgtc gagcacttcc ccggccaagc
     7741 gcaacaacga catcgactgc cagacacgaa gccactctgc aatcatgggc ttcctcgcca
     7801 gtctcgcaca ccatcttgcc cgatatcacg ggctactgcg aacgcctggc gcacgtccag
     7861 tacatcgtgg atgcgaagca ccgatgctcc ccgcgccgca cacgccaggc atgccgcgat
     7921 ggttcccccg attcgacgat tcggtgtcga ctggacacgg gccgcgagaa acgacttgcg
     7981 actgggcccc acgacgatcc catgtcccaa ggatcgaaac tcatgcagcc ttgcaaccag
     8041 ttcgatcgaa tgacgcgcgt tcttgttgaa tccgaggccc ggatcgaaaa acacatcctg
     8101 tgcatccaag cccgcttgca tcgcccgttt tgcggcttgt tgccattcgt tacgaacgtc
     8161 ttgaatcaca tcctggtagg catcatcatc gcacacggaa aatccttgca ttttcgacat
     8221 cggctcgcgc gcatgcatga tgatcagcgc ggccccttgc cgagccgcca cacgcgccaa
     8281 cgcatcacca tcgcgaaggc atgatacatc attgacgaca ctggctccga gccgaagggc
     8341 ctgttccgcg acctgcgcat cggtcgtatc gatcgatacg atgacacctc gggataccgc
     8401 atggacaatc gcatccgtcg ttcggcgaat ttgctcctcc gcaggtacgg gagcggatct
     8461 cggacgcgtg gattctcctc caatatcaat gagttgcgcg ccttccgcga ccagttcgtc
     8521 tacccttcgc agcgccgcat ctcgatcgaa atactcaccc ccatcggaaa aggagtccgg
     8581 agtgatgttc aggactccca tccaaagtgg atcgtcgttg tcgttccgtc gatccagcat
     8641 ctttcgaagg gcgctcacga cctacgggta gcagcgccga ccgggcgaag caagagcagc
     8701 ttttatcaga caacgacggt cggtcggaac gctcactctt gccatacgcg cccagtggtt
     8761 ctagcgtctg ttcctaggta gcccttatgg ttccgggccc accctagcgc tcccatgctc
     8821 cgcacgcgtg ctcaagccgg ttccggcgcc atcctcgtcc tgagcttggt cttcgccata
     8881 gcatttagcg ccatttgctc cttcgacttg gtcgtttctg cttggacccc gagcttcaat
     8941 gctcccgccg gcatcgccat tcgcgtccct tttggcgccc gtttcgtcga aaaaggcgac
     9001 gcaggctcgg cccaagtcgt ctacgaacgc gctcgcatgg cggtggctcc aggcaccatc
     9061 atcgaccaaa gcctcgcatc ccatcgagaa gcgctcgcgt acgagctggg acggcgtcct
     9121 ccaagctttg cttccctcgg agcccttttt ctcatctaca ccgccctatc gctattgctg
     9181 accacctata tcgtgcgcct ggggcaaaat aggctgcgcc tgcttcgctc tcaactcggt
     9241 gtttttattc ttattttact gatgatggtc gccaccaagg cgatcctgtt gttcaccaac
     9301 ctgcccgaat attggtctcc cattgccgcc gtgcccctat ggattgcgct ggccttcgac
     9361 cgacgaagcg ccctgctgat caatatcgtt accgcctttc tcgccgcctc gttgctgcgg
     9421 ttcgacatga tgttcctcgc catcgctttg gtgcgaggaa tgaccgtcat tcttttgctc
     9481 atgaaccggc gaaaacccgt gagcctgctg gtcaccagcc tcatgggagg cattgcctcg
     9541 gcccttctat atattgccct gagtaccgtg ctcgagggaa attttgattt aattcgcgat
     9601 cttggacgtc tcgagggatc ctatatcctg tcttgcatcg gtggcggcgc gcttgctgga
     9661 ctgcttgcca cggtgttacg agatccggtg gaacggctgc tcggtcacgt accccgcgac
     9721 aaactattgg acttgaccga tatcgaacac cccttgcttc gaaaactggc tagtgaagct
     9781 cccggcacct gggagcactc tcgcgccatg gccaatctcg cggaaggcgc tgcttctgcc
     9841 atcggcgcgg atgctttgct cacgcgggtc ggcgcctact accacgactt gggcaaaacc
     9901 gtccaaccaa cctttttcgt cgaaaatcag ggcgaaggtc cttcacccca tgatgacttg
     9961 cctcccgagg tatccgccga cgccatcatg gcccatgtcg tgctcggcac caaaatcctt
    10021 cgcgaaggtg gtatccccga acccgtcgtc gaattcgtct acacccatca tggcacccaa
    10081 ctcgtcgagt atttctggca caaatacctg caacaaaacc ccgaacccgc cctttccaaa
    10141 gaccattttc gctacccggg aatgaaaccc caaaccaaag aaaccgcgat cctgatgctc
    10201 gtcgattcca
//
LOCUS       DAOSOR010000155        10183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6585_length_10183_cov_4.696880, whole genome shotgun sequence.
ACCESSION   DAOSOR010000155 DAOSOR010000000
VERSION     DAOSOR010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 10183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10183
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6585_length_10183_cov_4.696880"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2931
                     /gene="nifJ"
                     /locus_tag="PK710_10295"
     CDS             <1..2931
                     /gene="nifJ"
                     /locus_tag="PK710_10295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOT10151.1"
                     /translation="VLRGTAQNPDVFFQHRETMNSFYTNCPGIVQKHMDRFAKLTGRH
                     YHLFDYVGAPDAEDVVVVMGSGAEAVHEVVEHLVAKGRKVGMVKVRLFRPWSIKDFLA
                     ALPKTTKRIAVLDRTKEPGAIGDPMYQDVQSSFVEALANDSCPLPTMPRIYAGRYGLG
                     SKEFNAGMVLAVFDNLVAPKPKNHFVCGIVDDVTHTSLDWDNDFDSEAPGTVRALFYG
                     LGSDGTVGANKNSIKIIGEETDNYAQGYFVYDSKKAGSMTVSHLRFGAKPIRSTYLLT
                     KADFVACHQFVFTEKFDMLKYAVEGGTFLLNSPYSADEVWDKLPKLVQQRIIDKKLKF
                     YAIDAYSVAKATGMGARINSIMQTCFFAISGILPREEAIAKIKTAIKKTYGVKGDVIV
                     EKNWKSVDQTLEHLHQVKVPDKVTSQIEMPPVVSPEAPAFVRNVLGPMIGALGDSLPV
                     SAFPIDGTYPTATSQWEKRNIALEVPIWIPELCIQCGKCAIVCPHAAIRTKVFPPESL
                     VDAPETFQVMDFKDRDLAGMKYSIQVFVEDCTGCRMCVEACPGKDKKNPSRRSLEMAD
                     QIPLREPERKNAAYFMRLPDADRTKVKQITVKGSQLLRPLFEFSGACAGCGETPYVKL
                     LTQLFGDRAIIANATGCSSIYSGNLPTTPYAVNEEGRGPAWSNSLFEDNAEFGFGFRL
                     AVDKFKVQAEESLVRSDIAKSHPQLVEQIKNAVQIEEPEILEQRARVAELKKILATKS
                     DPESKALLTLADYLVKKSIWIVGGDGWAYDIGYGGLDHVLASGRNVNVLVLDTEVYSN
                     TGGQMSKATPVGAVAKFAAAGKPIQKKDLALISASYGYIYVATVAMGANDAQTVRAFV
                     EAEAYDGPSIIIAYSHCIAHGIDMAMGMTNQKRAVESGHFPLFRYNPDLAAKGENPLK
                     LDSKAPKIPFEEYAYAETRWKMLTKSKPERAKELLRLAGENAQKKWLYLDQLAKMSYE
                     VPGK"
     gene            3268..4371
                     /locus_tag="PK710_10300"
     CDS             3268..4371
                     /locus_tag="PK710_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10152.1"
                     /translation="MSKLRSLTVLFSVWMLGMPSCGSGEEQDAASGGAGAAGSGGQEQ
                     GGTGAGTTGGGGSGGSGGAEQGGTGGSGGEGNFDPNNPGIRMTFTVGVALLMGQPATF
                     AAGAFLPTAREDLEAGPNQPSVPLGSCEETTTTYTPTCTGPQDCAPEQQCLPETDMNG
                     KPIANSESCVTPRSPIDVGPFTMTGFASGPKTLSYNAQQKGAYTTPGGDGTIPYGDLG
                     FDRIYEFEGAGDASKGLGSFHGKVRLGPEFRLTSPSMVSLPIGVDGIQVSVSQDLALT
                     WSGADQDKELTITLTGATMNGQSHTITCRTMDTGAFTIPAAKVQAAQLGDMAFLNMLT
                     FERNERGEATGEGMTSHDISTMQTAVINVAKLP"
     gene            4540..5457
                     /locus_tag="PK710_10305"
     CDS             4540..5457
                     /locus_tag="PK710_10305"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HOT10153.1"
                     /translation="MNDWQEHLEKTSRTFALSIPLLDEPLRREVTIAYLLFRVADTLE
                     DAFVWPSTMRRSALLACGEIVRSGDYQQATRMARQWGESSPTTHAGYLALLAAFPSLL
                     EDQRALRPKAARCIEHHLVRTIDQMADFATNWVEDEVLTLRTRDDLRAYCYAVAGIVG
                     ELLTELFILHSPSLRSAADSLSRLAPCFGEGLQLVNIAKDSVDDVKEGRSLIPSSMTT
                     EDVIGLAHTDLDRADLYVQLLRRGGAPPGTIAFAALPVALARASLQKISKMGAGAKLT
                     RAEVAELWGRVVALSTMPIDSLGFEAEMS"
     gene            5467..6048
                     /locus_tag="PK710_10310"
     CDS             5467..6048
                     /locus_tag="PK710_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10154.1"
                     /translation="MIDGVVARKNGKLTLFAKVGQRMGVVACVLMALGCTDDAEQDFK
                     RCEQLDKERKYEEALLACQEAQKKDSRSPFGQKAISLESKLHDKISALAQEKAAAAKE
                     SEEADALETANAKVVFNQVSTPPNDPQGYSERCMARNRAYENSYHCEPKDPSSVREGD
                     AFPFKEECMLIAKNRGCMPFYEDSPTKLFCCTK"
     gene            complement(6214..8211)
                     /locus_tag="PK710_10315"
     CDS             complement(6214..8211)
                     /locus_tag="PK710_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT10155.1"
                     /translation="MRKHQNTSERNGARTIFQVVLLCSRDRRSNQMSANYFLDNKDLR
                     FQFEHGIDWKEIVELTEAGFKLPDGHASLEDAKEFYAMVMESVGEFAGDFIAPRALKI
                     DTQGNHLVDGKVVSGEALVEVYEGLRDRELLGMPIPRELGGSNCPMCVFFATGEVLGR
                     ADVSVMTHFGFHGGIGLALLMYAAHEGSLQVENGQVTQTRWDDVIREIAAGQSWGAMV
                     LTEPDAGSDLGRIRAKAEKRDGKWYLTGEKIFITSGHGQWHIVLAKTDIDDPKAKKDP
                     LKQLSLFMVPQMIERDGETIRNFEVTKVEEKVGHHGSATCSLQYENSECELIGKEGEG
                     FKLMLTLMNNARVGVGFESIGVCEAAVRQAREYAAQRVTMGQTIDHHPIVAEMLMDME
                     LDIVGLRALAFSAANCVELASKLEMKLLSDPPTDPDERKAMKRKAKKYGMQGRLLTPL
                     LKYAAAEAAVRIARQNMQVIGGPGYISEIGADKLLRDALVLPVYEGTSQIQALMCLKD
                     NMTQALSKPGPFARDAARLTFLATTAHDETEKLFYKAEASLHQAMLTILARIASNKLR
                     RTVADKGITEIGQFAKMSNWDPKTDFTQGLMHAERFTRAKADVEMARILLQQAKEFPE
                     RRLIAERFMRRMAPRVAKEVDAILSNDTTVLDWIADLDKQS"
     gene            complement(8261..9184)
                     /locus_tag="PK710_10320"
     CDS             complement(8261..9184)
                     /locus_tag="PK710_10320"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOT10156.1"
                     /translation="MHPRIRKLRTIATFGQVWASGFTMRLGLRAKRDRMLRPRISGVV
                     PAFSEAEIERYILSTGGALDEYRDSDGSLLIPPTYFATWGLAELGKALLASCLPLNFS
                     RVVHASSRVRVHRLQKVNEPARFSATVETVVRTGKKVRIEQQLTTMTPEAQPLAEHTV
                     ALVIPDGRSKRRTEQSADIVPVDSTLLSAIRLPPHEGWRYARLSGDFNPIHWASPIAK
                     LLGWRGPIAHGFDLMARTCHASVRHLAQGDARKLRKLDVTFRRPVHLPAELHLFAGQA
                     TAMGQTKRVLLWVGAEQEAPAKITGHVEVDG"
     gene            9549..>10183
                     /locus_tag="PK710_10325"
     CDS             9549..>10183
                     /locus_tag="PK710_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase PrkA"
                     /protein_id="HOT10157.1"
                     /translation="MSQEDRIQRCKHHMDRIAASFEERFHHGRLQLSFSEYLDLFSSD
                     PRRYGRDAAMYLKDMFDHYGTESVIRPTGALTRWKLFDLPWESGPEAHRFALVGQEDV
                     QAEVYRVLANFVRGGRANRVMLLHGPNGSSKSTVAACIMKALEDYSLKEEGALYRFHW
                     VFPNEKRSRGPLGFRETSKDGHRVTDEASYAHWADEDLDARLLIEVRDHPI"
BASE COUNT         2221 a         2888 c         2862 g         2212 t
ORIGIN      
        1 gttttgcgag gcacggcgca gaacccggac gtgttctttc agcatcgcga gaccatgaat
       61 agcttttata cgaactgccc tggcatcgtg caaaagcaca tggatcgttt cgccaagctg
      121 accggacggc actaccactt gttcgactat gtcggtgctc ccgacgccga agatgtcgtg
      181 gtcgtcatgg gctctggcgc ggaagccgtg catgaggtgg tggagcatct cgtggcgaag
      241 ggaagaaaag tcggaatggt caaggtccgt ctcttccggc cttggagcat caaggatttt
      301 ctcgccgcat tgcccaaaac caccaagcgc atcgccgttt tggatcgaac caaagaaccc
      361 ggcgccatcg gcgatccgat gtatcaagat gtccaatcct ccttcgtgga ggcgctcgcc
      421 aacgattcct gtccgctgcc caccatgccg cgcatctatg ccggtcgcta cggattgggt
      481 tccaaggagt tcaacgcggg aatggtgttg gcggtcttcg ataacctcgt cgcccccaaa
      541 cccaagaacc acttcgtttg tggtatcgtc gacgatgtca cccatacctc cctcgattgg
      601 gacaacgact tcgactccga agctcccggt accgttcgcg ccttgttcta cggcctcggc
      661 tccgatggta ccgtcggcgc caacaaaaac tccatcaaga ttatcggtga ggaaaccgac
      721 aactatgccc agggctactt cgtgtacgac tcgaagaaag ccgggtccat gaccgtttcc
      781 catcttcgtt tcggcgccaa acctatccgt tctacctatc ttcttaccaa agcggatttc
      841 gtggcatgcc atcaattcgt cttcaccgaa aaattcgaca tgctcaaata tgcagtcgaa
      901 ggcggcactt tcctgctcaa ttctccgtat tccgccgacg aggtatggga caaactcccc
      961 aaactcgtcc agcagcgcat catcgacaaa aaactcaagt tctacgccat cgacgcttac
     1021 tccgtcgcca aagccaccgg gatgggagcg cggatcaaca gcatcatgca gacctgcttc
     1081 ttcgccatct ccggcatcct cccgcgcgag gaggccatcg cgaaaatcaa gacggccatc
     1141 aagaaaacct acggcgtcaa gggtgatgtc atcgtcgaaa agaactggaa gtccgtcgac
     1201 caaaccctcg aacacctgca tcaggtgaaa gtgcctgaca aggtcacgag ccagatcgaa
     1261 atgcctcccg tggtgagtcc ggaagcgccg gctttcgtgc gtaacgtctt gggccccatg
     1321 attggtgccc tcggtgactc gttgcccgtc agtgctttcc ccatcgacgg tacttatccg
     1381 acggctacga gccagtggga aaaacgcaac atcgctctgg aagtcccgat ttggataccg
     1441 gagctgtgta ttcaatgcgg aaagtgcgcg attgtttgcc cccacgcggc cattcgaacc
     1501 aaggtattcc cgcccgagtc cttggtcgac gctcctgaaa cgttccaggt catggatttc
     1561 aaagaccgtg accttgccgg aatgaagtat tccattcagg ttttcgtgga ggattgcacc
     1621 ggctgccgga tgtgtgtcga agcctgcccg ggtaaagaca agaaaaatcc gtcgcgccgt
     1681 tctctcgaaa tggccgatca aattccattg cgcgagcccg agcgaaagaa cgcagcctac
     1741 ttcatgcgtc tgccggatgc ggatagaacg aaagtcaagc agatcacggt caagggcagc
     1801 caattgctcc gtccgttgtt cgagttctcc ggagcttgtg cgggttgcgg cgaaacaccc
     1861 tacgtcaaac tcctcacaca attgtttggg gatcgggcca tcatcgccaa tgctaccgga
     1921 tgctcttcga tttatagcgg caacttgccg acgactccct atgcggtcaa cgaagagggt
     1981 aggggaccgg catggtccaa ctccttgttc gaagacaacg ctgagttcgg attcggattc
     2041 cgcctggcgg tcgataagtt caaggttcaa gccgaagagt ccctcgtgcg aagtgacatc
     2101 gccaaatccc atccgcaact cgtggaacag atcaaaaacg cagtccaaat cgaggaaccg
     2161 gaaatcctcg agcagcgtgc tcgcgtggcc gaactcaaga aaatcctcgc caccaaatcc
     2221 gatcccgaga gcaaagcctt gctcaccctc gcggactacc tcgtcaaaaa gtccatttgg
     2281 atcgtgggtg gcgacggctg ggcttacgat atcggctatg gtgggctcga ccacgttctt
     2341 gcttcagggc gaaacgtcaa tgtcttggtg ctcgataccg aggtctattc caacaccggt
     2401 gggcaaatgt ccaaggcaac tccggtgggc gcagtggcca agttcgccgc ggccggtaag
     2461 ccgatccaga aaaaggatct cgcactcatc tccgcttcgt atggctacat ctacgtggcc
     2521 accgtcgcca tgggtgccaa tgatgcccag accgtacgtg ccttcgtgga agcggaagct
     2581 tatgacggtc cctccatcat catcgcttac tcccattgca tcgctcacgg catcgacatg
     2641 gccatgggga tgacaaacca gaaacgtgcg gtggagtcag gccacttccc gctgttccgt
     2701 tacaatccgg accttgccgc caagggggaa aacccgctca agctcgatag caaagcgccg
     2761 aaaatcccct tcgaggagta cgcctacgcc gaaacgcgct ggaagatgct caccaagagc
     2821 aagcctgaac gagccaagga attgctgcgc cttgccggtg agaacgccca gaaaaaatgg
     2881 ttgtacctcg atcaactcgc caagatgagc tacgaagtgc ccggcaagtg acctctgtca
     2941 tgacctcccg ccgctttccg aattgatacc tctccccgtt ctatccgttt tgggatagct
     3001 atcgctgttc aggcaacatc tcggatgtcc cgaaaccagg cgcgccgccc catcgcatgc
     3061 ccgccatgag ccgagccata cgtctgctca cgacgtacat tttggtttct cgtgaagagg
     3121 ccgaaacccg cgctcgaaat atgtgtaact attggtatta taaggcaaat atccgatcac
     3181 gacgcttccg ccgtcatcgc tcgggtcgct ctcatactca ccgactccgg gctgcgctac
     3241 actgattcga ttcctttaga ggtggccatg tcgaaactac gttcattgac cgtgctcttt
     3301 tccgtgtgga tgctggggat gccgagttgt ggctcgggcg aggagcagga tgcggcttcg
     3361 gggggggccg gagctgccgg cagcggaggg caagaacaag ggggaacagg agcgggaacc
     3421 acgggcggtg gaggtagcgg aggttcgggc ggagcggagc agggaggaac gggcggctcg
     3481 ggaggcgagg gcaacttcga tcccaacaac ccaggtattc gcatgacatt caccgttggc
     3541 gtggctttgc tgatgggaca gccggccacg ttcgcagcgg gcgcctttct tccgacagca
     3601 cgagaagact tggaggcggg acccaaccag ccttccgttc cgttgggctc gtgcgaggaa
     3661 acgacgacta cctacacgcc gacctgtacc gggccccagg actgtgcgcc cgaacagcaa
     3721 tgcttaccgg aaacggatat gaacggtaag ccgattgcga atagcgaaag ctgtgtgaca
     3781 ccgcgttctc cgatcgacgt cggacctttt acgatgacgg ggtttgcttc cggtccgaaa
     3841 acgttatcgt ataacgcgca gcaaaaaggt gcgtacacca caccgggcgg agacggtacg
     3901 atcccgtatg gagaccttgg tttcgaccgg atttacgagt tcgaaggtgc tggtgatgcc
     3961 tcgaaagggc tagggtcatt tcatggaaaa gtacgtctcg gcccggaatt ccgcctcacg
     4021 tcgccttcca tggtttcttt gcccatcggc gtcgatggga ttcaagtcag cgtcagtcaa
     4081 gacctcgcat tgacatggtc tggtgccgac caagacaagg aactgacgat tacgttgacg
     4141 ggtgccacga tgaatgggca aagtcatacg atcacgtgcc gcacgatgga taccggcgct
     4201 ttcaccattc cggcggccaa ggtgcaagcc gcgcagcttg gcgatatggc atttctgaac
     4261 atgctcacct tcgagcgaaa cgagcgggga gaagctaccg gtgaggggat gacttcccac
     4321 gatatctcga cgatgcagac cgcggtcatc aacgtggcca agctcccctg agggggcgag
     4381 cggcgcttcg gtcaccatcg gccattcggt ttagagccgt caggggtaag gcggatcgtt
     4441 gaaccatgac aagccgtcat ttcctgtttc gttgtggttg tgttgcgctt gacatctcga
     4501 cgccgtggtt cgaggtagcc cccataagaa cgggtgctca tgaacgattg gcaagaacac
     4561 ctcgaaaaaa cgagccgcac cttcgcgctt tccattcctc tgctcgatga acccttgcga
     4621 cgggaggtga ccattgcgta tctgctgttt cgcgtggccg acacgctaga agacgccttt
     4681 gtgtggccgt cgaccatgcg gcggagcgct ttgcttgctt gcggcgaaat tgtccgttca
     4741 ggagactatc agcaagcaac gcgaatggcg cgtcagtggg gggagtcgtc ccccacgacc
     4801 catgcgggct atcttgcttt attggctgcg tttccttcat tgttggagga tcaacgagcg
     4861 ttgcggccga aagcggcgcg atgcatcgag catcatctgg ttcgcaccat tgaccagatg
     4921 gcggacttcg cgactaactg ggtggaggat gaagtattga cgcttcggac gcgggacgac
     4981 ctgcgagcgt attgttatgc cgtggcgggt attgtcggcg aattgctcac ggagcttttc
     5041 attcttcatt ctccgtcgtt gcgctcggcg gcagactcgt tgtcgcgtct cgccccatgt
     5101 ttcggtgagg ggcttcagct tgtcaatatc gccaaggatt cggtggatga cgtcaaggag
     5161 ggcaggagcc tcatcccgtc ttcgatgacc acggaagatg tgattgggct ggctcacacg
     5221 gatctcgacc gagcggacct atatgtgcag ctccttcgtc gtgggggagc gccgcctgga
     5281 accatcgctt ttgccgcgct tcccgtggcc cttgcccgtg catccttaca aaaaatctcg
     5341 aaaatgggag caggagcgaa gctgacccgc gccgaagtcg cagaactctg gggtcgtgtc
     5401 gttgcccttt ccacgatgcc gattgactct cttggtttcg aagccgagat gagctagggg
     5461 cacatcatga tcgatggagt cgtggctaga aaaaatggga agctgaccct attcgcgaaa
     5521 gttgggcagc ggatgggtgt cgtcgcttgc gtgttgatgg cgcttggatg cacggatgac
     5581 gcggagcagg acttcaagcg ttgcgaacaa ctcgacaagg agcgcaagta cgaagaggct
     5641 ctgttggcgt gtcaggaggc gcaaaagaag gactcacgaa gcccatttgg acagaaagcg
     5701 atctcgttgg agagcaagtt acacgacaaa atttcggcgt tggcgcagga aaaagccgcg
     5761 gcggcaaaag agagtgaaga agccgatgct ctcgaaacag cgaatgccaa agtggtattc
     5821 aaccaggtat cgacgcctcc caatgatccg caaggctatt cggaacgttg catggcgcgt
     5881 aaccgggctt atgaaaacag ctatcattgc gagccgaaag acccttcctc cgtgcgcgaa
     5941 ggtgatgcct ttcctttcaa agaggaatgc atgctcattg cgaaaaaccg cggctgtatg
     6001 ccgttttatg aagatagtcc caccaagctg ttctgttgca ccaagtagct tctccctcac
     6061 ccttgtcgga ttcgttcgcg gatgaaggag cgatacgcct ggccgcgcga ctcttgcgac
     6121 aggtgcaaac gttgcgtctc ggtcgaagag cgatcgaaga ggagatcgcc ttgtggctga
     6181 ctcgatggct tgcggggatg ctggcaggag cggttacgat tgcttatcga gatccgcgat
     6241 ccaatcgagt acggtggtgt cgttgctcaa aatcgcatcg acttccttgg cgacacgggg
     6301 ggccatgcga cgcatgaagc gttcggcgat gagacgtcgt tcggggaatt ctttggcttg
     6361 ttgcagcagg atgcgcgcca tttccacatc ggccttggca cgcgtgaatc gctccgcatg
     6421 catcaggccc tgggtgaagt cggtcttcgg gtcccaattg ctcattttcg cgaactgacc
     6481 gatctccgtg attcccttgt cggccacggt gcgacgcaac ttgttggaag cgatgcgcgc
     6541 caggatggtg agcatggctt gatggaggct ggcttcggct ttgtaaaata gtttttccgt
     6601 ttcgtcgtga gcggtagtgg caaggaaagt gagacgagcc gcatcgcgcg cgaatggtcc
     6661 tggcttggat agggcttgcg tcatattgtc cttcaagcac atgagagctt gaatttgaga
     6721 ggttccttcg tacacgggca acaccaaggc atcgcgcaaa agtttgtctg cgccgatttc
     6781 ggaaatatat ccgggcccgc cgatcacctg catgttttgg cgtgcgatac gaacagcagc
     6841 ttctgccgcg gcatatttga gaagcggcgt gagcagtcgc ccctgcatgc cgtatttctt
     6901 ggcctttcgc ttcatggcct tgcgttcgtc cggatccgtc ggtggatcgg atagaagttt
     6961 catctccaac ttggaagcga gttccacgca gttagccgcg gagaaagcga gggcgcgcaa
     7021 tccgacgatg tccaattcca tatccatgag catttccgcg acgatgggat ggtgatcgat
     7081 cgtttgcccc attgtcacac gctgtgccgc gtactctcga gcttgtcgca cggcagcttc
     7141 gcacacaccg atcgactcga atcccacacc gacgcgcgcg ttgttcatca aggtcagcat
     7201 gagcttgaag ccttcacctt ccttgccgat gagttcacat tcactgtttt cgtactgcaa
     7261 cgaacacgtg gccgaaccgt ggtgtccaac tttttcttcc accttggtga cttcgaagtt
     7321 gcgaatggtc tcgccatcac gctcgatcat ctgcggcacc atgaacagac tcaattgctt
     7381 gagcgggtct ttcttcgctt tcggatcatc gatatcggtt ttcgcaagca cgatgtgcca
     7441 ctgaccgtgt cccgaagtga tgaagatttt ttctcccgtc agataccact tgccatcgcg
     7501 tttttctgct ttggcgcgaa tacgtccaag gtcgctgccc gcatccggtt ccgtaagaac
     7561 catcgctccc caactctgtc ccgcggcaat ctcgcgaatc acgtcgtccc accgcgtctg
     7621 agtcacttgt ccgttctcga cctgaagact tccctcgtgg gccgcgtaca tcaacagcgc
     7681 caacccgata ccgccatgaa aaccaaaatg cgtcattacc gaaacatcag cccgtccgag
     7741 tacctccccc gtggcgaaaa atacgcacat cgggcagttc gaccctccca actcgcgcgg
     7801 tatcggcatg ccaagcaact cgcgatctcg cagcccttcg tacacttcca cgagagcctc
     7861 accgctgacc accttgccat cgacgagatg attgccctgc gtatcgattt tcaacgcgcg
     7921 tggtgcaatg aaatcacccg cgaactcgcc cacggattcc atcaccatcg cgtagaattc
     7981 cttggcatct tcgaggctgg catgaccatc gggcagcttg aagccggctt ccgtaagctc
     8041 tacgatctct ttccagtcaa tgccgtgttc gaactggaat cgcaggtcct tgttgtccag
     8101 gaagtagttg gcgctcatct gattggatct cctgtctcgt gagcagagca agaccacttg
     8161 aaaaatcgtt cttgccccat ttcgttccga agtattttgg tgtttacgca ccgcggcatg
     8221 aaataagcac aacccaccat gagaaggcta cgaagggcca ttacccatcc acctccacat
     8281 ggccggtgat tttcgctgga gcctcttgct ccgcccccac ccaaagcaat actcgcttcg
     8341 tttgccccat cgcggttgcc tgtcccgcga acaggtgaag ctccgcggga aggtgaacag
     8401 gacgacgaaa agtaacgtcg agtttgcgaa gttttcgtgc atcgccttgg gcaagatgac
     8461 gcacgctcgc atgacacgtt cgtgccatca ggtcgaaacc atgcgcaatg ggccctcgcc
     8521 agccgaggag tttcgcaatc ggagatgccc aatggatggg attgaagtca ccggaaagac
     8581 gcgcgtagcg ccagccctca tgggggggta agcggatggc ggacaacaat gtcgaatcga
     8641 cgggcacgat gtcggcgctt tgctccgtgc gccgtttcga tcgtccgtca gggatgacca
     8701 aggcaacggt atgttcggca agcggttgcg cttctggggt catggtggtg agctgctgct
     8761 cgatacgcac ctttttcccc gtacgcacca cggtctccac ggtggcggag aaacgggcag
     8821 gctcattcac tttctgcaag cgatggactc gaacgcggct cgaagcgtga acaacacgcg
     8881 aaaagttcag cggtaggcac gaagccagca gggctttgcc aagttccgct agcccccagg
     8941 tcgcaaaata agtaggcgga atgagcaaag agccatccga gtcgcgatac tcgtcaagcg
     9001 ctccgcccgt actcaaaata tagcgctcga tctccgcttc cgaaaatgct ggaacgactc
     9061 ctgaaatgcg tggacgaagc attcgatcac gcttggctcg gagtccaagc cgcatcgtga
     9121 accctgaggc ccacacctgt ccgaatgtgg cgatggttcg cagtttgcgg attcgcggat
     9181 gcatcggcgc gaagcctagg acgtttggca cgacgcgtca tcaggaagat gcatgaggag
     9241 agggcagcct cgaaacgaac ccgctttcac gacgcaatgc catgccttgt cccattttcg
     9301 accgtgagca tgtcgcgttg ccgaatatcg tccaattgct gataactatt gaaaatattt
     9361 aggcttttta gggaccgggc gtgggattgg aaaggcgttc gtagctcgag gcacagtcga
     9421 tggcagaaag ctgcgtttca ggacttgcgc gcttacgtca tgcaaatggc actgaaagga
     9481 taacgcgcag gggacaggct ttccacaacc ccatcaacca tcgttccaaa agtgataccc
     9541 tgtcagcgat gagccaagag gatcgaattc agcgctgcaa acatcacatg gatcgcattg
     9601 cggcttcctt cgaggaacgc tttcatcacg gtcgactgca actgtcgttt tccgagtatc
     9661 tcgacctgtt ttcctccgat ccaagacgat atgggcgtga tgcggcgatg tatctcaagg
     9721 atatgttcga ccactacggc accgagtcgg tgattcggcc caccggtgct ctcacacgat
     9781 ggaaactctt cgatcttccc tgggaatcgg gccccgaagc ccatcgtttt gcccttgtcg
     9841 gacaagaaga cgtacaagcg gaagtgtacc gcgtccttgc caacttcgtg cggggaggcc
     9901 gcgccaatcg ggtcatgctg ctgcacggtc cgaatggaag ctcgaaaagc accgtggccg
     9961 cctgcatcat gaaagccctc gaagactaca gtctcaaaga agagggagcg ctgtatcgct
    10021 ttcattgggt gtttcccaat gagaagcggt ctcggggtcc gttggggttt cgagagacca
    10081 gcaaagatgg tcaccgcgta accgatgagg cttcctacgc gcattgggcg gatgaagact
    10141 tggatgcgcg tttgctcatc gaagtgcgcg atcatccaat ctt
//
LOCUS       DAOSOR010000156        10140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6628_length_10140_cov_4.709172, whole genome shotgun sequence.
ACCESSION   DAOSOR010000156 DAOSOR010000000
VERSION     DAOSOR010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 10140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10140
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6628_length_10140_cov_4.709172"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PK710_10330"
     CDS             <1..467
                     /locus_tag="PK710_10330"
                     /inference="COORDINATES: protein motif:HMM:NF040596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MXAN_2562 family outer membrane beta-barrel
                     protein"
                     /protein_id="HOT10158.1"
                     /translation="WGYTKMSAKAKLTGDGGDSAETTGLWIMPMYGVAVLRIDVLANE
                     VGIPLVPYLKGGVGYALWKTSDGAGLSTYRQGDETIEGKGRSYGWHFSPGIMLQLDPF
                     DRHAAQQLDNSVGVNHSYFFLEWMRSQLDGFGKNDQMRVGTSTWAAGLAFEF"
     gene            842..1438
                     /locus_tag="PK710_10335"
     CDS             842..1438
                     /locus_tag="PK710_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT10159.1"
                     /translation="MANENDHELMHRAREGDRQAFGELVRRHQQRIFRLAMHMLHDTT
                     EAQDVTQETFVRAYQALSRFQGNSQPYTWLYRIAVNLSLNIIRMRKSRRSEVDDDDPR
                     IEAWLSVHGSSGLRGPAVANEQRELAKALCEGIDQLSETLRTTLLLVAVDGVGQAQAA
                     EVLGCSEGTVAWRVHEARRKLRLHLAHLGWMDGTEGEP"
     gene            1435..2745
                     /locus_tag="PK710_10340"
     CDS             1435..2745
                     /locus_tag="PK710_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10160.1"
                     /translation="MSDSHEMDRKLDGWLADWPVPERSDREWEEFGSKLDERLSGLTI
                     GHGDDAWLQPPLPDQPGEGDREKLLAGGVSMSERNSERPPKKSLKDFAQRVSAIPTAD
                     TKSAPISSVGTPIPSSGPRASSPSLRASVERISHPPEARDSDSGIVDLNAVRAKDEPD
                     SGAQPAETGLFDGETKAPAPAPAAKKTSMAPVVGGGIVAVLALAAATVLVVRTSEKSA
                     PNHLESTPAVASVAATPAAEHAGLASPRSASNTDQELSVERLGVAESSGESAEGQPFG
                     ANAAPGRAGGSAAADKAKTEASVADKDGKKEDSPATPADTKDLAGAMATAVGAHESKK
                     DDGKKSSTSSVDPGSLPETPSQGAVQGAMGSVRDAARACVAGQTESSRATITFGSSGS
                     VSSVSVSGGAAGTPAAGCIQAALKRARVGPFKKDSFTVSMTIRP"
     gene            complement(2755..3774)
                     /locus_tag="PK710_10345"
     CDS             complement(2755..3774)
                     /locus_tag="PK710_10345"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOT10161.1"
                     /translation="MNRTLRVGVVGAGAWGCALADQLAKKSYSVSLWCFEAEVAQSIA
                     ESRINEIYLPGHRIHPLVSPTTDLLSCVQDKDLVVVAVPSHHVRTIARQIGPALHRDS
                     LLVSASKGIENDSLMTMSGVYSDALDINFHKIYCVLSGPSFAMEVAKGMPTAVTVAAW
                     DHRTAKRVQEIFSTTYFRCYTSTDVVGVELGGALKNVVAIAVGAVDGMGFGHNTRAGL
                     MTRALSQIARIGVPLGASPLTLSGLSGAGDLILTCTGDLSRNRTFGVRLGKGESVDAI
                     LSGQPQVVEGFLTSKSAYHLIRQLGVEAAIFEQIYEYLHENRDHKPIAHFVHQVMSRD
                     LKPEW"
     gene            3773..3994
                     /locus_tag="PK710_10350"
     CDS             3773..3994
                     /locus_tag="PK710_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10162.1"
                     /translation="MTEPPRCWVLHGTEHDRTVRINLDTQGKKQTGQWLEIVVGPRIE
                     RHENASADREDPGNKRQNGTIVGTQDKTD"
     gene            4248..4520
                     /gene="rpsT"
                     /locus_tag="PK710_10355"
     CDS             4248..4520
                     /gene="rpsT"
                     /locus_tag="PK710_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOT10163.1"
                     /translation="MANHPSAERRNRQRIKQTNRNRSIKSSTRTALRKARAALAAGDV
                     KLAETLVRDVESRLDRATTKGVLHRKAASRVKSRLSTQLHKLSTAS"
     gene            complement(4601..5221)
                     /locus_tag="PK710_10360"
     CDS             complement(4601..5221)
                     /locus_tag="PK710_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT10164.1"
                     /translation="MAIERDMKLLTASDVASLCEVDLKTIHNWVDRGCIPHFRTPGRH
                     LRFQAVDVANFLRDWGYAIPRKFYAQIAQTVLVIGSPEAVAVVTRAAGQSATIRSVED
                     LCEAAVRVGAEPTSAVVVEASVVHANPQAFIMMVEAVKRAFQVPVHVLGEDTTNLAEK
                     IDVLFVPMGDSKMLRAAMTGDVDDPAQIEAGILPSPPTRRKSRRRE"
     gene            complement(5382..6830)
                     /locus_tag="PK710_10365"
     CDS             complement(5382..6830)
                     /locus_tag="PK710_10365"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOT10165.1"
                     /translation="MLLLGCGDLAQAPQRANTSSRGEPKVDCIPDIESDGKIAPIVQF
                     RVRNLNDAPKSPPRLVEGKLSERNLRDLRQGNPSEALQKRIIESITWTIEGQWYLSAE
                     RPLSLGGVYSLVVPETGWHKPWEVIERDEIPTLRRVWPPEQRAGMTAVWCLTWPALEL
                     APSEPQHLQFPPGIDGTLQGGAVDGVGVGCSQWTAHVGGAVVVPAPLIWWQQSWVRLD
                     PTPLSLQQFEENWQASCPSGQTPLGPVCAVISDNKAILGAKEPWLVGLTLPDFVQADA
                     VGAARPWTVKPLPPSSEIRGQARIMSVSGQVRNYEVHWKTAEPCARVVINEVMANPVG
                     PEPQQEWVELFNDGSQAINLQGWRLEDAGGGVELPAVTLQPGQFGLIVGQGYDPASWV
                     DAAPAEHTVFLPVTSVGIAGLSNAGEPLRLVDHEGQAVSFVPPIASKRPGGSIIRLRP
                     EAPDIPASFAVDLQGGTPGAPNRIPNPSENFP"
     gene            complement(6921..9020)
                     /locus_tag="PK710_10370"
     CDS             complement(6921..9020)
                     /locus_tag="PK710_10370"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT10166.1"
                     /translation="MLPAVKLPLVIGRYVLEGQIASGGMATVYYGRRVDENQFAKPVA
                     IKRMHPHCARDADFVAMFLDEANLAARIRHPNVVPVLDFAAEGEDLIIVMEYLAGDTV
                     GSLIQSAHEAKLYPPHRVVAAIITDVLKGLHAAHEVLGQDGQPLHIVHRDVSPQNIMV
                     GFDGVARVLDFGVAKASVRFQTTRAGQLKGRLQYMAPEQIRSRPVDRRADIYSTSVLY
                     WEMIAGRPLFDADNAGSMMMMVLEGTVPSLRAVDPSVSPALEWLILKGLRADPTGRFH
                     TALEMINAIEEAVGIAPAEEVADWVHRVAPASVRERVARISGAEVQHPIPAPLPAPGP
                     VPSSSSQAVAPPPKPTPRPKTKKLFVYAFGATFVAAIIAFWFALHHVPWLGPSLISVS
                     RTVFGDRFTAWIERVAHTADAGWAELQENDERPEAFWNLPANSATTVARFRPADMQPL
                     ISAPFARADGSWIPTETTSSPAVSFHTVLHLNGDHQDILARVVTIDRQNTKLVFAPAR
                     SARDGNEKTPADAATSPDDSWLLSIGLPTDSLGTGVDGAEISPALPNLCTLAELRDHS
                     VHINLWPQLLPLMPRMSWWRQTASCLVLAGTNAPALDTHARERSAALGISDNTNTLFV
                     VVGEKVDSDALARVMRYVGARFALQLDAPQRQVSVVMRKSEQRLEPLSPDPPTNPETN
                     PPPAPTYDTFYVFRSDR"
     gene            9265..>10140
                     /locus_tag="PK710_10375"
     CDS             9265..>10140
                     /locus_tag="PK710_10375"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOT10167.1"
                     /translation="MDAIRIRGGRPLHGRVRVSGAKNAALPILCTALLSSGESRLRNV
                     PNLRDINTLAALLRFLGRDTEVSEGVVRVGAEQGTIRPEAPYELVKQMRASVLVLGPL
                     LARYGKAKVSLPGGCAIGARPIDQHLKGLEAMGANIELDRGYVIAKGPKAMGGRLHGA
                     EYRFDVNTVTGTENLMMAAVLAKGRTVLDNCAREPEVEELAHTLNKMGAKVQGAGTSQ
                     IRIEGVEALEAFDHAIVADRIEAGTFMVAAAATAGNVLIEQAPMEHLEGFISKLRDAG
                     CRVEREGDGVRVTRQG"
BASE COUNT         2197 a         2655 c         3268 g         2020 t
ORIGIN      
        1 gctggggata tacgaaaatg agcgccaaag cgaagctcac cggcgatggt ggcgactcgg
       61 cggaaacgac gggactttgg atcatgccga tgtacggggt agcggtgctt cgcatcgatg
      121 tattggcgaa cgaagtgggg atacccctgg tgccctacct caaagggggg gtgggatatg
      181 cattatggaa aacgagtgat ggggccgggt tatcgaccta ccgacagggt gatgagacca
      241 tcgagggcaa agggcgctcg tacggttggc atttttcgcc ggggatcatg ttgcaactcg
      301 atccgttcga ccgtcatgcg gcccagcagc tagacaattc ggtgggcgtg aatcattcgt
      361 actttttctt ggaatggatg cgttcgcagc tcgatggttt tgggaaaaac gatcaaatgc
      421 gtgtgggaac gtcgacatgg gcggcgggtt tggcctttga attctgaggg atgacggggg
      481 gttcgagctg atcatcgggt atctccctcc gggccggcag gaaaaaataa atcgtgtaaa
      541 atcgctgggt tggctcgaca cgattcggac cgagggcaaa agggggccgt gaggcgatcg
      601 ccttgtagta ccgcgtattt tttcggtgcc acggcatggg cgggggagaa gttcgggggg
      661 acggcctggg ggcacgcgga tggggggttt gatttcgatg tcctaaagtt ggtcccgtcc
      721 aagatcctcg tggttgaatt gtccaaggcc acgtgttcga ttatcgtgcc aggcagatcc
      781 gtagtggccg cggacgttct ccccaagacg accgggcatt ggttttttgt gaatatcggc
      841 catggccaac gaaaacgacc acgaactcat gcaccgcgcg cgcgaaggcg atcgtcaggc
      901 gtttggcgag ctggtgcgga gacatcaaca gcgcattttc cgcttggcta tgcatatgtt
      961 gcatgacacg acggaggcgc aggacgtgac ccaggagacg ttcgtgcgag cttatcaggc
     1021 tctttcgcgt tttcagggca acagccagcc gtatacatgg ctgtacagga tcgcggtgaa
     1081 tctatcgctc aacatcattc gaatgcgcaa atcgcgtcgc tcagaagtcg acgacgacga
     1141 tccgcgtatc gaggcttggt tgtcggttca tggctcctcg ggcttgcggg gacccgcggt
     1201 agccaacgag cagcgggagc tggcgaaggc gctttgtgag ggtatcgatc aactcagtga
     1261 gacattacga accacgttat tgcttgtggc ggtggacggc gttgggcaag cgcaggcggc
     1321 ggaggtgctg ggttgttcgg agggaacggt ggcatggcga gttcacgaag cgcgtcgtaa
     1381 gcttcgattg caccttgctc atcttggttg gatggacggc acggagggcg agccatgagc
     1441 gatagccacg agatggacag gaagctcgac ggatggcttg cggattggcc ggtgccggag
     1501 cgcagtgata gggagtggga ggaatttggg agcaagctcg acgagcggct ctcgggattg
     1561 acgattggcc atggcgacga tgcctggctg caaccccctt tgcctgatca accgggcgag
     1621 ggagacagag aaaagctgct tgcaggaggc gtcagcatgt cggagcgtaa ttctgaaaga
     1681 ccccccaaaa agtcgctcaa ggacttcgcg cagcgcgtga gcgcgatacc cacggccgat
     1741 accaaatctg ccccgatttc gtcggtggga acgcccattc cgtcgtcagg tcctcgcgcc
     1801 tcatcgccat cgttgcgggc ctcggtggaa cgcatttctc atccaccgga agcacgagac
     1861 agtgattcag gcatcgtcga tttgaatgcg gttcgtgcca aggacgaacc ggattctggg
     1921 gcgcaacccg cggaaacggg gctattcgat ggggaaacga aagcacccgc gcccgcgcct
     1981 gccgcgaaaa aaacgtccat ggcgccagtc gtcggcggtg gcatcgtcgc tgtacttgct
     2041 ctggctgccg cgacggtgct cgtggtccgc acatcggaaa aaagcgctcc taaccatctg
     2101 gaatccacac ccgccgtggc ttccgtagcg gcaacaccag ccgcggaaca cgctggtctg
     2161 gcgtcgccgc ggtcggcatc gaatacggat caggagttgt cggtggaacg tttgggtgtt
     2221 gccgaatcca gtggcgaatc ggcggaggga caacctttcg gggcaaatgc tgccccgggt
     2281 cgagccgggg gttccgcggc agcggataag gccaagaccg aggcaagcgt tgctgacaag
     2341 gacgggaaaa aagaggattc acctgctacg ccggctgata ccaaagacct tgcgggagca
     2401 atggccacgg cggtgggggc gcatgaatcc aaaaaggatg atgggaaaaa gtcctcgaca
     2461 tcgtcggtag atccgggatc gttgccggaa acaccgtctc agggcgcggt tcagggagcg
     2521 atgggatcgg ttcgtgatgc ggcccgagcg tgtgtggcgg gacagacgga gtcatcgcgt
     2581 gcgacgatta cgtttggttc tagcggttct gtatcgagcg tgtcggtgtc gggaggagcg
     2641 gcgggcacgc cggcggcggg gtgtatccag gcggcgttga aacgtgcgcg ggttgggcct
     2701 ttcaaaaagg actcttttac ggtgagcatg acgattcgtc cgtaggggat tggtttacca
     2761 ttctggtttg aggtctcggc tcatgacctg gtggacgaag tgggcgatgg gtttgtggtc
     2821 tcgattttca tgtaagtatt cgtaaatctg ttcaaaaatg gctgcctcca caccgagttg
     2881 ccgaatcaaa tgataggcgg acttggaggt gaggaatcct tcgacgactt ggggttggcc
     2941 tgagaggatg gcatcgacgg actcgccttt tccgagtcga acgccgaagg ttcgatttcg
     3001 gctgaggtcg ccggtacacg taaggatgag gtcaccggcg cctgacagac cggatagggt
     3061 gagggggctt gcgccgaggg gcacaccgat tcgcgcgatt tgtgataagg cacgagtcat
     3121 gagtccagcg cgcgtattgt ggccgaagcc catgccatcg accgcgccca cggcgatggc
     3181 gacgacgttt ttcagcgcgc cgccgagttc cacccccacg acatcggtgc ttgtgtaaca
     3241 acgaaagtag gtggtggaaa aaatttcttg cacgcgtttg gccgtccgat gatcccaggc
     3301 ggcgacagtg acggcggtgg gcattccttt cgccacttcc atggcaaatg atgggccgga
     3361 aagcacgcaa tagattttat ggaagttgat gtcgagggca tccgaataca cgccggacat
     3421 ggtcatgagc gaatcgtttt cgattccttt ggaagcactg acgagcaagg agtcgcggtg
     3481 caacgcgggc ccgatttgtc gcgcgatggt tcgcacgtgg tggcttggca cggcaacgac
     3541 caccaggtct ttgtcttgga cgcaagacag caggtcggtg gtgggcgaga cgagagggtg
     3601 gattcggtga ccgggcaggt agatttcgtt gatacggctt tcggcgatgg attgcgccac
     3661 ctcggcttcg aagcaccaaa gcgacacgga atagcttttt ttggcgagtt ggtccgccag
     3721 agcgcagccc caggctccgg cccctaccac tccgacgcgc aatgttctat tcatgaccga
     3781 gcctccgcgt tgttgggttc tgcacggaac agaacatgat agaaccgttc gaatcaatct
     3841 cgatacgcaa gggaaaaaac agactggaca atggctagaa atcgttgtcg gccctcgcat
     3901 cgaacgtcat gaaaacgcct ctgctgatcg cgaggatccg ggaaacaaaa ggcagaacgg
     3961 aacaatcgtt gggacgcaag acaaaaccga ttgacaagtc gaccattgag gcgaatactg
     4021 cgccgccctg tcggtcccat cggcaggtgg agtggatcat ggtatcacct acgcagcaaa
     4081 cggaatcgat ccgacagcga aaagcgcgaa ggcgctgcaa aaacaacgca atagcgctca
     4141 agcgtcgtcc tacgccccat tttccgattc acctcgaggg ctacgacact tcggcccctg
     4201 acgccaagcc cactgcgcaa tccagcgaga agtagcgaga agagaagatg gcgaatcatc
     4261 catcagcgga acgccggaat cgtcagcgga tcaagcaaac gaaccggaac cgatcgatca
     4321 aaagcagcac acgcacggca cttcgtaaag cgcgagccgc gttggcggcg ggcgatgtca
     4381 aactagcaga gacactcgtg cgggatgtcg aaagccgact cgaccgtgca accaccaagg
     4441 gtgtcttgca cagaaaagcc gcctcccgcg tcaaatcacg cctgtccacg cagcttcata
     4501 agctctcgac agctagctga ttgctcacgc cacccatcgc ccttcgctgc tctaccaagg
     4561 gcgctcttgg tttcatctat ccaaggtcgg atgattgcgc ctattcgcga cgacgagatt
     4621 tgcgtcgcgt tggcggggag ggaagaatcc ccgcttcgat ctgagctgga tcgtcaacgt
     4681 ctcccgtcat ggccgcccga agcattttcg agtcacccat gggaacgaag agtacatcga
     4741 ttttttccgc cagattggtc gtatcttccc caagaacatg cacgggaact tgaaacgcgc
     4801 gttttaccgc ctcgaccatc atgatgaaag cttgtggatt tgcatgaacc acagaagctt
     4861 cgaccacgac agccgaagtg ggttcagcgc ccacgcgcac ggcggcttcg cataaatctt
     4921 cgacagatcg gatggttgcg gactgcccag ccgcgcgtgt gacgacagca acagcttcgg
     4981 ggctgccgat gacgagaacg gtttgtgcga tttgtgcgta aaacttccgt gggatggcat
     5041 atccccagtc acgaagaaag ttcgctacgt ccaccgcttg aaaacgtaaa tgccgccccg
     5101 gcgttcgaaa atggggaata caccctcgat ccacccaatt atgaatggtc ttcaggtcca
     5161 cttcgcatag agaggcgaca tccgatgctg tcagcagctt catgtccctt tcaatggcca
     5221 tttcccgcgt cctctcccgt gttgcaacgt ccggtggtca taccataacc gatgaaagac
     5281 tcgacgaata tagcacaagc agagcatcgg caggaatggg aaaatcaaac tcgttcaacc
     5341 aattgtcgag cgcaagggga atcaaggtag gttgcaacga attaagggaa attctccgag
     5401 gggttgggaa tacggttggg agcaccgggc gtgccgcctt gcaagtccac cgcaaaggaa
     5461 gcggggatgt ccggggcttc cggtcgcaag cgaatgatcg agccgccagg acgtttggag
     5521 gctatggggg gaacgaagga aacggcctgg ccttcgtggt cgaccaggcg caggggttca
     5581 cccgcgttgg acaagcctgc gatgccaacc gaggtcacgg gaaggaaaac ggtatgttcc
     5641 gcgggtgccg catcgaccca gctcgcaggg tcatatcctt gtccgacgat gagcccgaat
     5701 tgacctggct gcaaggtcac ggccggcagt tcgacacccc ctcccgcatc ctccagccgc
     5761 catccttgta ggttgatcgc ctgggagcca tcgttgaaca attccaccca ttcttgttgt
     5821 ggttcaggac ccaccggatt ggccatcacc tcattgatga ccacgcgcgc gcatggttca
     5881 gccgttttcc agtgtacctc gtaattacgc acttggccgg atacggacat gatgcgtgcc
     5941 tggcctcgga tctcagacga tggtggcagt ggtttcacgg tccaaggccg tgcggcacca
     6001 acggcgtcgg cttgaacgaa atccggcagg gtgagtccca ccagccaagg ctccttggcg
     6061 ccgaggatcg ccttattgtc ggaaatcacc gcacaaacgg gaccgagggg cgtttgcccg
     6121 gaaggacaag aagcttgcca attctcttcg aattgctgga gcgatagggg cgtaggatcc
     6181 agacgaaccc acgactgttg ccaccatatc aacggagcag gaaccaccac cgctccccca
     6241 acatgcgcgg tccattggct gcagcccacg ccaacaccat ccaccgctcc cccttgcaaa
     6301 gtaccatcga ttcccggagg aaattgcagg tgctgcggct ccgaaggcgc cagttcgagc
     6361 gcgggccagg tcaaacacca taccgccgtc attccagcgc gctgctcagg aggccagacg
     6421 cgccgaaggg ttggaatttc atcccgctcg atgacctccc atggtttatg ccatcccgtt
     6481 tcgggaacca cgagagaata gaccccgccc aaggataacg gacgttcggc acttaaatac
     6541 cattgcccct caatagtcca agttattgat tctattattc ttttttgaag cgcctcgctc
     6601 gggtttcctt gtcgcaggtc ccgaagattg cgttcggaca gttttccttc gacgagccgc
     6661 ggcggggatt tcggggcgtc gttcaggtta cgaacacgaa attgtacgat gggagcaatt
     6721 ttcccatcgc tttcgatgtc gggaatacaa tccaccttgg gttcgccgcg actgctcgtg
     6781 ttcgctcgct gaggggcttg cgccagatct ccacatccga gcagcagcat gctgcaaagc
     6841 aacctgattc gagcatgagt acacctcatg caaggcacat cggacgaatc gtctgcgggt
     6901 tgcgtgaaat cggcgccagg ctatcggtcg gaacgaaata cgtagaaggt gtcgtaggtg
     6961 ggagccggag gcgggttggt ttccggattg gtaggggggt cgggagataa aggttcgagc
     7021 cgttgttctg attttcgcat aacgacggag acttgccgtt ggggggcgtc cagttgcagg
     7081 gcgaaacgag cgccgacgta gcgcatgacg cgggccaggg catcagaatc gactttttct
     7141 ccaacgacga caaaaagtgt attggtattg tcgctgatgc ccagagcggc actacgttcg
     7201 cgcgcgtggg tatccaaggc tggcgcgttg gttccggcaa gcacgaggca ggaggccgtt
     7261 tgtctccacc atgacatgcg aggcataagc gggagcagtt gtggccatag attgatatga
     7321 acggagtggt cacgaagttc ggccagcgtg catagattgg gcaaagcggg cgatatttcg
     7381 gcgccatcga ccccggttcc gagcgaatcg gtgggcaagc caatgctcaa aagccatgaa
     7441 tcatcgggcg aagtcgcggc atcggcaggc gttttctcat tgccgtcgcg tgcggaacgg
     7501 gcgggggcaa aaacgagttt tgtgttttgc cggtcgatgg tgacgacgcg agcgaggatg
     7561 tcctggtggt cgccattgag atgcaagacg gtatgaaagc tgacggcagg cgaggaagta
     7621 gtttcggtgg gaatccacga tccatcggcg cgggcaaaag gcgcggagat gagcggttgc
     7681 atatccgcgg gacgaaagcg ggcaacggtg gtggcactgt tggcgggtag attccagaaa
     7741 gcctcgggac gttcatcgtt ttcttgtagt tccgcccacc cggcatcggc ggtatgggcc
     7801 actcgttcga tccacgcggt gaatcgatca ccgaataccg ttcggctgac gctgatgagc
     7861 gatggtccga gccatgggac gtggtgcaag gcgaaccaaa aggcgatgat cgcggccacg
     7921 aaggtggcgc cgaacgcgta gacgaaaagt tttttggttt tcgggcgagg cgtcggcttg
     7981 gggggtggtg cgaccgcctg cgatgaactc gatggcacgg gccccggagc gggaagcgga
     8041 gccgggatag gatgttgtac ctcggcgccg gagatgcgcg cgacacgttc acgaacggaa
     8101 gcaggggcca cgcgatgcac ccaatcggcg acttcttcgg cgggagcaat tcccacggct
     8161 tcttcgatcg cgttgatcat ttcgagcgcc gtgtggaagc gcccggtcgg atccgctcga
     8221 agtcccttta aaataagcca ttcgagagct ggcgagacgc tcggatccac ggcacgaagg
     8281 gatggtaccg taccttcgag gaccatcatc atcatgcttc ccgcgttatc cgcatcgaac
     8341 agcggacgtc cggcaatcat ttcccaataa aggacgctgg tcgaatagat gtcggcgcgt
     8401 cggtcgacag ggcggcttcg gatttgttcg ggcgccatgt attgcaagcg tcctttgagt
     8461 tgcccggcgc gcgtggtttg gaatcgtaca gacgctttgg cgacgccgaa gtcgagaacg
     8521 cgagcgactc catcgaatcc gaccatgata ttttggggag acacatcgcg gtggacgata
     8581 tgcaagggct ggccatcttg tcccaggact tcatgggcgg catgcaaccc cttaagaacg
     8641 tcggtgatga tggcggctac gacgcgatgg gggggataca gctttgcttc gtgcgcggat
     8701 tggatgaggc tgcccacggt atcgcccgcg aggtattcca tgacgatgat caggtcttcg
     8761 ccttcggctg cgaagtcgag gaccggaacg acattggggt gtcgaatgcg tgccgcgagg
     8821 ttggcctcat cgaggaacat ggccacgaag tccgcatcac gcgcgcaatg gggatgcatt
     8881 cgcttgatgg ccaccggttt ggcaaattgg ttttcgtcga ctcgacgtcc gtaatacacg
     8941 gtcgccatac cgccggatgc aatctgtccc tccagcacat agcgtccaat gacgaggggc
     9001 agtttgacag cggggagcac ggcggctcat catacctcgt accgcgagga atcgtagaca
     9061 tgatcgcgcc atgaagcggt tgatggatgc gaagggaccg ggctatccgt ttttgtctgg
     9121 gtaaatgatc tgagtttttc ttcgggggca gccgcgcccc gcaggggggc acaatcggtt
     9181 cgacgatggg ctaaggcggc gagggggact tgttctggat gtgctcttgg ggcaggctgc
     9241 gcccacaaag gaatttgcga tgtcatggac gccataagaa ttcggggcgg ccgtcccctt
     9301 catggccgag ttcgcgtcag tggagcaaaa aatgcggcgc tgcccattct atgcacggcg
     9361 ctgttgtcga gcggggaaag ccgtttgcgc aatgtcccaa acctacgtga tatcaatacg
     9421 ttagcggcgc ttttgcggtt ccttggcagg gataccgagg taagcgaggg ggtcgttcgt
     9481 gtgggagcgg aacaaggaac gattcggccg gaggcgccgt acgagttggt caaacaaatg
     9541 cgagcgagcg tgttggtact cggaccgctt ttggctcgat atgggaaggc gaaggtatct
     9601 cttccggggg ggtgcgcgat tggcgccagg ccgatcgatc agcatctcaa agggttggaa
     9661 gccatggggg caaacatcga gctggaccgt ggttacgtca tcgccaaggg cccgaaagcg
     9721 atggggggcc gattgcatgg cgcggaatac cgattcgatg tcaataccgt cacgggaacg
     9781 gaaaacctga tgatggcggc cgtattggca aaaggccgga cggtgttgga caattgtgcg
     9841 cgagaaccgg aagtcgagga gttggcacac acgctcaaca agatgggagc caaggttcag
     9901 ggggccggga cgagtcagat tcgaatcgaa ggggtggaag cgctcgaagc ttttgatcat
     9961 gccatcgtgg ccgatcgaat cgaggcggga acgttcatgg tggcagcggc ggccaccgcg
    10021 ggcaatgtac tcatagaaca ggctccgatg gagcacttgg aaggatttat atcaaaactg
    10081 cgagatgcgg gatgccgggt ggaacgtgaa ggcgatggag tgcgagtgac acgacaaggg
//
LOCUS       DAOSOR010000157        10057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6692_length_10057_cov_4.096881, whole genome shotgun sequence.
ACCESSION   DAOSOR010000157 DAOSOR010000000
VERSION     DAOSOR010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 10057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10057
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6692_length_10057_cov_4.096881"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="PK710_10380"
     CDS             <1..832
                     /locus_tag="PK710_10380"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOT10168.1"
                     /translation="IVPTLDPSWRAMTNRSAVTPWIVSLQTKNPIILQRFDMTFSVVL
                     AKDGRDRLVLSYPDKETGEVSLQGHAILYDGRIDVVGNRFDLEENNAWVIFTGDPAHP
                     QLNVTARWDAPDGTRVFADVTGPLRDPRIQFRSEPAMTQAEILGLLLFGPSSQGGATM
                     AAGTTEGEGNQSSDMSSGVSSGVAAAGINMLLQDLSPVVSTRFDTSRGQSPSPTLIVQ
                     VSRDVTAEATYVAEEATLDKADRYLVSFDWRFLRQWSLRITRGNVGTSVVDVLWQHRY
                     "
     gene            838..1737
                     /locus_tag="PK710_10385"
     CDS             838..1737
                     /locus_tag="PK710_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOT10169.1"
                     /translation="MTDCRAARLLVLLGFVLLGCSSNHHVSPWPRVESFQPLMTPSSL
                     EEQLERVASEMQSEGLTLVHEVCGRFDDVEGFCIRGYEGHDDLGHERNALRVATRYGV
                     VMALGPPSAEEAARGEGISWVVRLADRWDSGTDINDDGMPDVVVVRASKGLEIWGLHY
                     RGASPYPMDGVVPMTEAMDIDGDGRPELMAAVPTRAEPVKPQLVEILEFKSGRYRPDT
                     PLVRRYHAVKGEAMRDMPTEAAAVQMAWHRFRAGEDVADVLKALDERIARMGESSKAV
                     RASLRRWRNWLASTMAEERRGRE"
     gene            2130..3740
                     /locus_tag="PK710_10390"
     CDS             2130..3740
                     /locus_tag="PK710_10390"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOT10170.1"
                     /translation="MERRPLLGDEALALGAIHAGLSGAYSYPGTPATEIFEFIHHETR
                     KDPPAKDGGVHRVWSANEKVAYEEALGMSYAGRRAIVSFKHVGLNVAADPFMNSAVTG
                     IHGGLIVAVADDPGMHSSQNEQDSRVFANFALVPCLEPTTQQDAYDMARQAFDISEKF
                     GVPIMIRVVTRLAHSRSGVQVGERRKANELNPTTHAKGWTLLPQNARVQYAALVAKQP
                     EILAWSETCSWNKLRLNPQGGKIGYIVSGVAQNYFLANFEHDEQLPSHLVIGAYPIPK
                     ALIRRLFEAVDEVVVIEEGYPFIEDAIVGVLGPPSGKTVRGRFDGTIPRTGELDPDVV
                     RKAVGKEPLVHQPKPSLPIAGRPPRLCDGCPHIDAYDMISEMMDEHKELRVFGDIGCY
                     TLGALPPYNAAHSCVDMGASISMAMGAAQAGMRPVICTIGDSTFIHSGMTPLVGAAAQ
                     NTPMTVFILDNSTTAMTGGQDTMQTGPDLVRVVKGLGVDPEHIHVLEMRRKFHEENTN
                     VIRKELDYEGLSVIITVRECVITAAKKK"
     gene            3800..4633
                     /locus_tag="PK710_10395"
     CDS             3800..4633
                     /locus_tag="PK710_10395"
                     /inference="COORDINATES: protein motif:HMM:NF005324.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOT10171.1"
                     /translation="METNIILAGVGGQGIVSISYVIDMAALKSERFFKQAEVHGMSQR
                     GGAVSSHLRISDKPIHSDLVPRGKGTLVLSVEPLESLRYVEYLSPDGALVVSTDPFVN
                     IPNYGDVEEILDAIADIPSHTLIASDRLARQAGTAKAQNMVMLGAASPYLDFPDDVLE
                     NGIREAFAKKGEKVQGINVAAFRAGKAAGSAYRALIQNKVRSKQARSFIGRLKLGVLE
                     DDAIDSWSRVLTSEMAPAITELLEGKTLGQIRGTREIATRVLDASDASSRLPELLFQD
                     P"
     gene            4673..7033
                     /locus_tag="PK710_10400"
     CDS             4673..7033
                     /locus_tag="PK710_10400"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOT10172.1"
                     /translation="MSFDPTPIRRLLDSIAADNRFSLLEHEVYAVLEAAGCRVPAFIF
                     LSPSELPSEQALQRLGSSEVMIKVVSTQIAHKTDVGGVRKVEATPEAIRRAMREMLDT
                     IPTRFVQWLEAHPSLVPTDTSRSSPDAMRQAVHDSIRGFLVVEKVAMQQAGPGSEWLV
                     GLRHNREFGPVLTLGIGGVDTELLGEACRKGKAVVTGSADRLDISSLFALMKPSLAYQ
                     RLAGLTRGGQRLVSDSEIQRVIEAFHRIATVLGTDETEGWTITELEVNPFVVVDGTLV
                     AIDGLLKFRARSEPSSPRPAPSIDALLHPSSIGLIGVSSKGMNMGRIILRNVLDAGFD
                     KDRVYVVRPGQEQIDGVRCVPSVEALPERVDMFLLAVNADQVPDVIEDLAKHDKAVGV
                     ILIAGGMGEKQGGETIEARVKRALRDARSQGKPLVINGGNSLGVVSRPGKYHTLFIPP
                     NKLPLETTGRNNVAFISQSGAYMITRMSKMAWLSPRYAMSTGNQTDLSVTDYLAFLAG
                     DEALQTLAVYVEGFQDGDGLHFARAVEKAVASGKDVIFYKAGRTTEGKSATSGHTASL
                     AGEYDVCSAIVRRAGAHVAPSFEDFLHLVKLSSLLGHKRWAGPRLAAMSNAGYETVGI
                     ADSVRGEEWDLRLAQPNPDTRRKLEKALITGKIDSLVDVRNPLDVTPMANDQVHEEVM
                     RAFLDDPGVDMALCASVPLTPAMATLEHDSRPEWTLDSEGSLPNRLARVLTETDKPLV
                     GCIDSGELFDPMVRMLEAKGIPTFRSADVAIRTMGMYLAGRLRNGG"
     gene            7247..9079
                     /locus_tag="PK710_10405"
     CDS             7247..9079
                     /locus_tag="PK710_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT10173.1"
                     /translation="MRFKVVVDTVGLFALFVGALGLTSSAQAQPRVAGTNGEGMDTHL
                     FRPALDSKGFFTVNGADILGANDVSFGLVMDYGRNLMRLEDGHGDEQLVAHSFQGVFG
                     FNYGLFNVATLGISAPVHLMSGDPVEQVGPAAAPYDSGALDAQTFSGIALHSKLRLLR
                     PESGFGLALALQGGIPFSKATARNLGSDPKAWFWPQAIVETRVGSKDQLRIGANAGYR
                     IHDGKNPRFDQLEEGPFQYGNLLTGGLGISYRAMDALDLVADTYATQLFDGHSSSKQK
                     LSAEAVGGIKVFVESRSFLMLGAGRRYTPGFEAADLRLFIGFVFEPSIGDRDGDGIKD
                     DVDQCPDEPENYNGYQDEDGCPDVIPEPEEKPLPTVSDRDGDGIPDHEDKCPDEGGDV
                     IREKGPYYGCPDRDKDGIPDHLDSCPDEGGDVIRVPGPYYGCPDRDKDGIPDHLDKCP
                     DEPETYNGFEDEDGCPDVGKVVVEDNEILIMEKIMFETNSAEIRPESFDLLDAIATTL
                     KHHPEFLLVEVGGHADERGTDEHNLRLTRARSESVRKALIERGIADSRLKAMGYGEFC
                     PVDPAPNAEAWEKNRRVEFKILKTEDGDTGVATGCDRARQKGIQ"
     gene            complement(9163..>10057)
                     /locus_tag="PK710_10410"
     CDS             complement(9163..>10057)
                     /locus_tag="PK710_10410"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HOT10174.1"
                     /translation="SVGQLPHLSMHRIAVRTPNYITFSIPKRSGGNRLIQAPKRKLKA
                     AQKKVLSLLIHKLPVHEAAHGFRQGRSIRTGAEPHVHRRVVIRLDIKDFFPSLTFARI
                     RGYLIAMGYGYEVATTLACLCSEPQRQPVEVGGEIVHVPVGPRVAPQGAPTSPGLLNA
                     ICHKLDCRMQGLARAHGFAYTRYADDLTFSGDYPDRVGKILSRARAIIRSEGFEVNPS
                     KTLVMRRGKAQRVTGVTVNETLGLSRQQRRLVRAAIHQASFVSPMDGTSKARLLGKVA
                     YIQMLNQSQAEPLRRQTEKLT"
BASE COUNT         2100 a         2513 c         3177 g         2267 t
ORIGIN      
        1 aatcgttcct actctcgacc catcgtggcg agcgatgacg aaccgttccg ccgtaacacc
       61 ctggatcgta tcgttgcaga cgaagaaccc catcattttg caacgtttcg acatgacctt
      121 cagcgtggtg cttgcgaaag acgggcgtga tcgattggtg ctttcctacc ccgacaagga
      181 aacgggagag gtgtcactgc aaggtcacgc catcttgtat gacggtcgta tcgatgtggt
      241 aggcaatcgt ttcgatctcg aagagaataa tgcatgggtg attttcaccg gtgaccccgc
      301 gcacccccaa ctcaacgtga cggcgcgatg ggacgcaccg gatggaacga gggtttttgc
      361 cgatgtaacc gggcctttgc gtgaccccag gatccaattc cgaagcgagc cggcgatgac
      421 ccaagcggag attctcggcc tgttgctctt tgggccttcg agccaaggtg gggcgacgat
      481 ggcggcgggc accacagaag gcgaaggcaa tcagagttcg gatatgagca gcggtgtgag
      541 tagtggtgtg gcggcagcgg ggatcaacat gctgctgcag gacctcagtc ctgtcgtttc
      601 cacccgtttc gataccagcc gcgggcagag cccgagccca acgctcatcg tccaggtttc
      661 tcgtgatgtc accgcggaag ccacctatgt cgcggaagaa gccaccctcg acaaggctga
      721 ccgatatctc gtctctttcg attggcgttt tctccgtcaa tggtcgcttc gcatcacacg
      781 aggcaacgtg gggacatccg tggtcgatgt tctgtggcag cataggtatt gacgaggatg
      841 acggattgcc gagccgcacg tttgttggtt ttgttgggat tcgtgctgct tggatgcagt
      901 tcgaatcatc atgtgtcgcc ctggcctcgt gttgaatcgt tccaacccct gatgacgccc
      961 agctcgttgg aggaacaact cgagcgcgtg gcgtcggaga tgcaaagcga gggattgacg
     1021 cttgttcatg aggtatgcgg caggttcgat gacgtcgagg gattttgcat ccggggttat
     1081 gaagggcacg atgatcttgg ccacgagcga aacgcgctgc gtgtggcgac aagatatggt
     1141 gtggtgatgg cgcttggtcc tccttccgcc gaggaggctg cgcggggcga ggggatatct
     1201 tgggtggttc gacttgccga tcgctgggat tcgggcaccg atattaatga cgatgggatg
     1261 cctgacgtgg tggtggttcg tgcatcgaaa ggattggaaa tatgggggtt acattatcga
     1321 ggggcttccc cttatccgat ggatggggtg gtaccgatga cggaagcgat ggatatcgat
     1381 ggcgatgggc gtccggagtt gatggcagcg gtgccgaccc gagccgaacc cgttaagcct
     1441 caacttgttg aaattcttga gtttaaatcg ggaaggtacc gaccggatac gcctcttgtg
     1501 cggcgttatc atgcggtcaa aggcgaagcc atgcgcgata tgcccacgga agcggcagcc
     1561 gtgcaaatgg cctggcatag gtttcgagcg ggtgaagatg tggcggacgt gttgaaggcg
     1621 cttgatgagc gaatcgcgag gatgggtgag tcgtcgaagg cggttcgtgc atcgttgcga
     1681 cgatggcgaa actggttggc gtctaccatg gctgaagagc gtcgggggcg tgagtgaatg
     1741 ggattcgaaa gacggttgag gcaaggcagg gggatcctgg gtgcgcgtga ggtgttgtgt
     1801 cgttcgggga aaaatacgga tttcggcaag ttcgcaaggg ggggaaaggg ttgtacgatg
     1861 agggggaaaa ccgttcacgg ttgaatgggc tggaggtagg gtttgcgcga cgaccatggt
     1921 tgcccgttga aagacacgat agaattccgg tcgagtttta acgtgatggg agtggtcacg
     1981 ggtctcttac ggttcgttgc gccatgggtt tactttcgag cttcgaacca ttatgaacgg
     2041 ggttgaatat cgtgcagttg ccgccgaacg cgggcgctgc acgaggtgtt tgcataccgg
     2101 tcgcatgctt ggcgaccgat ggaggcacga tggaacggcg gccattatta ggcgacgaag
     2161 ccctcgcgct cggggccatt catgcgggat tgagcggtgc gtattcgtat ccgggaacgc
     2221 ctgcgaccga gattttcgaa tttatccacc acgaaacgag aaaggatcct cctgcgaagg
     2281 atgggggggt tcatcgcgtt tggtcagcga atgaaaaagt tgcgtatgaa gaagcgctcg
     2341 gcatgagtta tgcggggcgt cgtgccattg tgtctttcaa acacgtgggg ttgaacgtgg
     2401 cggcggaccc attcatgaac tccgctgtga cggggattca tggtgggctc atcgtggcgg
     2461 tggctgacga tccggggatg cattcgagcc agaacgagca agatagccgc gtgttcgcga
     2521 acttcgccct cgttccttgt ctggaaccga cgacccaaca agatgcctat gatatggcgc
     2581 ggcaagcgtt tgacatcagc gaaaagtttg gtgtcccgat catgattcgg gtggtgacgc
     2641 gccttgcgca ttcgcgttcg ggagtgcagg tgggggagcg tcgaaaggcc aacgaattga
     2701 atccgacgac tcatgcgaag ggatggacgt tgttgccgca aaacgcgcgt gtgcaatacg
     2761 ccgcgcttgt ggcgaagcaa ccggaaatcc tcgcctggtc tgaaacttgt tcctggaaca
     2821 agcttcggtt gaatcctcaa gggggtaaga tcggttatat cgtgtcgggc gtggcgcaga
     2881 attacttcct cgccaacttc gagcatgacg aacagcttcc ttctcatctg gtcatcgggg
     2941 cttatccgat acccaaagcg ttgattcgcc gccttttcga ggcggtggac gaggttgtcg
     3001 tcatcgaaga aggttatccg ttcatcgaag atgccatcgt aggagtgctt gggcctcctt
     3061 cgggcaagac ggttcgtggg cgattcgatg gtacgatacc gcgaacaggg gaactcgatc
     3121 ccgatgtggt gcgaaaagcg gtgggaaaag aaccgcttgt ccatcagcca aagccatcgt
     3181 tgccgatcgc aggacgtccg ccgcgtttat gcgatgggtg tccgcatatc gatgcctacg
     3241 acatgatttc cgagatgatg gacgagcaca aagagcttcg ggtcttcggc gatattggct
     3301 gttataccct tggcgcgctg cctccctata atgccgccca ttcctgcgtc gacatgggag
     3361 ccagcatcag tatggccatg ggggcagcgc aggcggggat gcgtccggtc atttgcacga
     3421 tcggagattc gacgttcatt cattcgggaa tgacaccgct ggtgggagct gccgcgcaga
     3481 atacgccgat gacggttttt atcctcgaca actccacgac ggccatgacg ggtgggcagg
     3541 atacgatgca aacgggtccc gacctcgttc gcgtcgtcaa aggtttgggt gtcgatccgg
     3601 agcatattca tgtgctcgag atgcgtcgta agttccatga ggagaacacc aacgtcattc
     3661 gcaaggaact cgattacgaa ggcctttccg tcatcattac cgtgcgcgag tgtgtcatca
     3721 ccgcggcgaa gaaaaagtag tctcgttcgt tcatatccgc gcgtgggggt tcgaaccacg
     3781 tgcatgggag aagaaactca tggagaccaa cattatcctc gcgggagttg gcggacaggg
     3841 gatcgtatcg atttcttatg tcatcgacat ggctgctttg aagagcgagc gattcttcaa
     3901 acaagctgaa gtgcatggca tgagccaacg cggtggcgcc gtcagctcgc atcttcggat
     3961 ttcggacaaa cccatccatt cggacctggt tcctcggggc aaaggaaccc tcgttctttc
     4021 cgtcgagcct ctcgaaagcc ttcgatatgt agaatatctt tcgcccgacg gggccttggt
     4081 ggtgagcacc gatccgttcg tcaacattcc caactacgga gatgtagaag aaattcttga
     4141 tgccattgcc gacattccat cgcatacgct cattgcatcg gataggttgg cgcgccaggc
     4201 cgggactgcg aaggcacaaa acatggtgat gttgggagcg gccagcccgt acctcgattt
     4261 tcctgacgat gtgctcgaaa acggaatacg tgaggcattc gcgaaaaaag gcgaaaaagt
     4321 gcaggggatc aacgttgccg cgttccgggc gggaaaggcc gcgggcagtg cctatcgcgc
     4381 gttgattcaa aataaggtgc gcagcaaaca ggcgcgaagc ttcattggca ggctcaagct
     4441 aggtgtgctc gaagatgacg ccatcgactc gtggtcgcgc gtattgacca gcgagatggc
     4501 ccccgcgatc accgagttgc tcgaaggaaa aacgcttgga cagattcgtg ggacgcggga
     4561 gatagccacg cgcgttctcg atgcctcgga tgcttcatcg cgtctgcccg aacttttgtt
     4621 tcaggatccc tgattccaga taaaccgtcc atttttgaaa cacgagatcg ccatgagctt
     4681 tgatcccact cccatccggc gcctgctcga ttcgattgcc gcggacaacc gcttcagtct
     4741 tttggaacac gaagtgtacg ccgtgctcga agctgccggt tgtcgcgttc ctgctttcat
     4801 atttctttct ccttccgaac tgccttcgga acaggcgcta caacgtttgg gctcctccga
     4861 ggtgatgatc aaagtcgtat ccacgcaaat cgcacacaaa acagatgtag ggggtgtgcg
     4921 aaaggtggag gcgactcccg aggcaattcg tcgggccatg cgagagatgc tcgacaccat
     4981 ccccacgagg ttcgttcaat ggttagaggc tcatccttcg ctcgtcccca cggatacgtc
     5041 tcgatcctcg ccagatgcga tgcgtcaagc ggtgcatgac agcattcgtg gttttctcgt
     5101 ggtggaaaaa gtagcgatgc aacaagcggg gccgggctct gaatggcttg tgggccttcg
     5161 tcacaaccgt gagtttggtc ccgtgcttac gctggggatt gggggagtgg atacggagtt
     5221 gcttggcgag gcgtgtcgca aaggcaaggc ggttgtgacg ggttccgcgg atcggctcga
     5281 tatttcctcg ttattcgcct tgatgaaacc ctcgcttgcg tatcagcgac tcgcgggatt
     5341 gacccgggga gggcaacgac tggtttctga ttcggagata cagcgggtca ttgaggcgtt
     5401 ccatcgaatt gccacggtat tgggtacgga tgagaccgaa ggatggacca tcacggaact
     5461 cgaggtgaat cccttcgtgg tggtggatgg aacgcttgtg gcaatcgatg gattgctcaa
     5521 gtttcgagcg cgttccgaac cttcctcgcc ccgtcccgca ccgtcgattg acgcgttgtt
     5581 gcacccgtcg agcattgggc tcattggagt gtcttcgaaa ggcatgaata tgggtcgcat
     5641 tatcttgcga aacgtgctgg atgcgggatt tgacaaagat cgtgtctatg tggtgcggcc
     5701 tgggcaggag caaatcgatg gcgtgcgttg cgtgccgtcg gtggaggcgt tgccggaacg
     5761 agtggacatg tttttgctgg cggtcaatgc ggaccaggta ccggatgtga tcgaggatct
     5821 ggcaaagcac gacaaggcgg tgggagtgat tttgatcgcg ggagggatgg gggagaaaca
     5881 agggggtgaa acgatcgagg cgcgagtcaa acgcgccctt cgtgacgccc gttcccaggg
     5941 aaaaccgctt gtgatcaacg gaggtaattc gttgggtgtg gtgtctcgtc ctggcaaata
     6001 ccacacgttg ttcattcctc cgaacaagct gccgctcgag accacgggac gaaacaacgt
     6061 ggcgttcatc agtcagagcg gtgcctacat gatcacgcgt atgagcaaga tggcgtggct
     6121 tagtcctcga tatgccatgt cgacgggcaa ccagacggat ttgagtgtga cggattattt
     6181 ggcgtttctt gcgggcgatg aggccttgca gacgttggct gtctatgtgg agggctttca
     6241 agacggagac gggttgcatt tcgcgagagc ggtcgagaaa gccgtggctt ctggcaagga
     6301 tgtcattttc tacaaagcag gccgcacgac ggagggcaag tcggcgacca gtgggcatac
     6361 ggcctcgctt gcgggcgagt acgacgtttg ctccgcgatc gtgcgtcgcg cgggggcgca
     6421 tgtggctcct agtttcgagg actttcttca cttggtgaaa ctatcgtctt tgctgggtca
     6481 taagcgctgg gccggaccgc gcctcgcggc catgtccaac gcgggctatg agacagtggg
     6541 cattgccgat agtgtgcgag gggaagaatg ggatcttcgg cttgcccaac ccaatccaga
     6601 tacgcgacgc aagctggaaa aggcgctcat caccggaaaa atcgattcgc tcgtcgatgt
     6661 tcgcaatcct ctcgacgtga ctcccatggc gaatgatcag gtgcatgaag aggtgatgcg
     6721 agcgtttctc gacgatccgg gggtggacat ggcgctatgt gcgtcggttc ccttgacgcc
     6781 cgcgatggcg acgctggagc acgattcgcg tccggagtgg acccttgaca gtgaggggag
     6841 tctgcccaac cgcctggctc gggtgctgac ggaaacggac aaaccgctgg tgggctgcat
     6901 tgacagtgga gagcttttcg atcccatggt gaggatgctc gaagccaagg gtattccaac
     6961 gtttcgttcg gcggatgtgg ccattcggac gatgggcatg tacttggcgg gtcggttacg
     7021 caacggtggt tgaaggcgct gtgggcattg ttgtcgaatg ctgggctgtg gtggcgcatc
     7081 gaccgatgat tgcgatgggc ggactacggt ggggggggcg tatctgccgt gcattgacat
     7141 cgccggtgag actctgatag tttcgctcct cccgaagtaa aaaagatctt tcttaacgag
     7201 atcttgccgc tataacgaac gccctttctt cccaccaaag gccatcatgc ggttcaaagt
     7261 cgttgttgat acagtcggtt tgtttgcgtt gtttgtgggg gcacttggtt tgacctcttc
     7321 tgcccaggca cagcctcgcg ttgcgggtac caacggcgag ggcatggaca cgcatctatt
     7381 tcgtccagct ttggattcca aaggattttt cacggttaat ggcgcggata ttcttggtgc
     7441 caacgacgtg agctttgggc tggtgatgga ttatggccgt aacttgatgc gtctcgagga
     7501 tggtcatggg gacgagcagt tggtggcgca ttcgtttcaa ggcgttttcg ggttcaacta
     7561 cggtcttttc aatgtggcta ctcttggtat ttcggcgccc gttcacttga tgtcgggaga
     7621 tccggtggag caagtggggc cggcagcggc gccgtatgat tcgggcgcgt tggatgcgca
     7681 gacgttctcg gggattgcat tgcattcgaa acttcgattg ctccggcccg aaagtggttt
     7741 tggcttggcg ctggctctgc aagggggaat tccgttttcc aaagcaacgg ctcgcaatct
     7801 cggatcggat ccgaaagcat ggttttggcc ccaggcgatt gtcgaaaccc gcgttggctc
     7861 gaaagaccaa cttcggatcg gcgccaacgc tggataccgc attcacgatg ggaaaaaccc
     7921 tcgctttgac caactcgaag aaggcccttt ccaatacggt aacttgctta ccggagggct
     7981 cggcatctct taccgggcca tggatgcgct cgacctcgtg gccgatacgt acgctactca
     8041 gctattcgat gggcattcga gttcaaaaca gaaactgtcg gccgaagcgg ttggcggtat
     8101 caaggttttc gtcgaatcgc gttcattttt gatgctcggt gccggtcgta ggtacacccc
     8161 gggcttcgag gccgcggacc ttcgcttgtt catcggcttc gttttcgaac catcgattgg
     8221 ggaccgagac ggggatggta tcaaggacga tgtggatcaa tgtccggacg agcccgagaa
     8281 ttacaacggg tatcaggatg aagacggctg tccggatgtg attccggagc ccgaagaaaa
     8341 accgctaccc accgtatccg atcgcgatgg cgatggtatt ccggaccatg aggataaatg
     8401 cccggatgaa ggtggcgacg tcatccgcga aaaaggcccg tattatggtt gtccggatcg
     8461 ggataaagac ggcatccccg atcatctcga ttcgtgtccg gatgaaggtg gcgatgtcat
     8521 tcgcgtgccg gggccgtatt acggttgtcc ggatcgggat aaagacggca tccccgatca
     8581 cctggacaag tgtccagatg agcccgaaac ctacaacgga ttcgaggatg aagacggctg
     8641 cccggacgtc gggaaggtgg tggtcgagga caatgaaatc ctcatcatgg aaaagatcat
     8701 gttcgagacc aacagcgcgg aaattcgtcc ggaatcgttc gacttgctcg acgcgatcgc
     8761 gacgaccctc aagcaccatc ccgagttcct gctcgtggag gtgggaggcc atgccgacga
     8821 gcgaggaacc gatgagcaca acctgcgctt gacccgagcc cgctctgaat ccgtgcgaaa
     8881 agctttgatc gagcgtggta tcgccgattc ccggctcaag gcgatgggct atggggagtt
     8941 ctgccctgtc gatccagctc ccaatgcgga agcctgggaa aagaaccgtc gcgtcgaatt
     9001 caagattctc aaaaccgaag acggtgacac aggcgttgct accggctgcg atcgcgctcg
     9061 acaaaaaggc attcaataag cgattcaacc gcggctggaa ttcccccttc gaggctcatg
     9121 catgcccaac gaaccaaccg tcttgcgtgg gtttgacgcg cgtcaggtca atttttccgt
     9181 ctgacgacgt aggggttcgg cttgcgattg gttgagcatt tggatgtaag cgaccttgcc
     9241 gagcaagcga gctttgcttg tgccatccat gggagacacg aacgaagctt ggtgaatcgc
     9301 ggctcgaacc agacgtcgct gttgtcggga taaccccaag gtttcgttga ctgtgacgcc
     9361 tgtcactcgc tgtgctttgc cccttcgcat gacgagcgtt ttgcttggat tgacctcgaa
     9421 tccttcggaa cggatgatcg cacgggcgcg agagagaatc ttaccaaccc gatccggata
     9481 gtcacccgaa aacgtgaggt catcggcata gcgtgtgtac gcaaacccgt gagcgcgtgc
     9541 gagcccttgc atacggcaat cgagcttatg acatatcgcg ttcagaagtc ctgggcttgt
     9601 cggtgctcct tgcggcgcga cccgtggtcc gacgggcacg tggacaatct cgcctccgac
     9661 ttcgaccggt tgacgctgtg gttcggaaca caaacaagcg agggtggttg ccacctcgta
     9721 gccatatccc atcgcgatca aatacccacg tattcgagca aacgtgagag aaggaaaaaa
     9781 atctttgata tcgagccgga tgaccacgcg tcgatgaaca tgcggctccg ctcccgttcg
     9841 aatggaccga ccctgtcgaa aaccgtgggc agcctcatgg acgggaagct tgtggatgag
     9901 aagggaaagc acttttttct gagcggcttt cagcttacgt ttgggcgctt gaatcaaacg
     9961 gtttccacca gaacgcttgg gaatggaaaa tgtgatgtaa ttgggtgtgc gtaccgcgat
    10021 gcgatgcatc gataggtgag gaagttgacc tacgctg
//
LOCUS       DAOSOR010000158        10032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6713_length_10032_cov_4.315626, whole genome shotgun sequence.
ACCESSION   DAOSOR010000158 DAOSOR010000000
VERSION     DAOSOR010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 10032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10032
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6713_length_10032_cov_4.315626"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2733
                     /locus_tag="PK710_10415"
     CDS             <1..2733
                     /locus_tag="PK710_10415"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT10175.1"
                     /translation="GRNLCSIVQARGVLPVSQVFSTALDILDALEDMHRQGIVHGDIL
                     PECILIGATEEGEVRAKVVNHVLGRLASVAIPRLACGGTASGTSRYMAPELAMGEPPS
                     VFSDIYSFGAVLYQMLTGIPPRRSSCCLLMERQWQKPVVPPSMVAKDRTISSAVDDVL
                     LRALDREPSRRFSSASEMRQVLWEAKNESPAVISTPTITVPSNGHRLFGRQQALDIVH
                     TIALMPVAYGTEDQGHPRGAVAVVVGSAGVGKTALLRAVRDRLEGSPVAVLRVDGRRA
                     LTRPLEPFITLVQDVLGVRRGDEPKTIDEVRKALVLRFGIEEQDCLRLLERVTDRESS
                     LSLTPDIIEREQVRSLRAFLGRMLASRPTVLVVEDADAMDTASLELTRDLMEASSTLA
                     LSVLVSCRSDPYPDWDAGYVTRLHLDALDEMASKSMLEHRTMGMVVPSDAMAMAVQWS
                     KGVPLLLELTSRAMRQREFLVEPDGTFKAPSTSQDLFGGLRQLVSIALRDRPVCVRQW
                     IFVVAIAGGAPIELLEAYESSQGAIEETIGACLETGWVSYSDEVMEFDNEGVRDVVRS
                     MIPEGTKRQMHAFVAKWLQQTTPARAPGEVIASHLVMGGDVLGAAQAYEKEAIALSSR
                     EEHVAATVIWGRAAQAWQMLRDEQALCRVAIARTEAFLAAGNGKAATEELSALERFEP
                     AGLDGLRARAMAAVASSQSNWDLAIQSLLRASALAVDNLDQQAWFDVEFCLGELLYRR
                     GELGAAESHATTCLDLAISIADSRGERATLVDGARIAQAATFLSKLRIETNRMDKARN
                     ALAMSLEHTAILGDEASASRLLANLAYACEPEDLEASLSYAKRALELALRAGDRMAAA
                     RIAINLGAYQAQSHAFEDALASYAMAKKLALAVGWARGVRLAKSAAEKIHSTSS"
     gene            complement(2770..3378)
                     /locus_tag="PK710_10420"
     CDS             complement(2770..3378)
                     /locus_tag="PK710_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT10176.1"
                     /translation="MPIELPTVRILIVDDDKAICDYMQTLLERDRFEVKTINDPFLVE
                     DELRQGHYHLIILDLMMPNMDGIELLGRIRKIDKDVAVVIFTGYPNLETAVASMKLDA
                     VDYVKKPFNVDEFREVLDRVMRKKGLSRTPEEQLHKVIGESIRSLRKEKELTLKQMSR
                     RTGLSVSLLSQIERAESSASLSSLYKIAAALDVRVQDLFGDY"
     gene            3642..5237
                     /locus_tag="PK710_10425"
     CDS             3642..5237
                     /locus_tag="PK710_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10177.1"
                     /translation="MIRFDKHSPWLDRAIVLGLYLLAVAVLAYHARQAGIGADESAYL
                     DAGRRVGEWFDRIGSAESSFFTQADRDAVWAPVHAVVNASTLLSALVWHVAFSGFDVL
                     GECVSLRLGTVLLNAFSVPLLYALLTPGLGRKVGGLSVLLWMLVVRAQHEASLANPGV
                     VAMTGSLAVLVAHGRARSAGRENRMGKSAAWMAVAGLLLGVGVAVSHATIVVLLVLFT
                     NSLWLQRKRLREMLRGGDVPVPSSMVFFAIFAPLAYGLATPWLWHDTAARIRPMLVRA
                     FSPGTQAQGIGHTALSLVLSTPMVIVLGALVGIAVTIGPSRWRGWGKKETTGDDETPA
                     LLIVGLLTGLGWTWLLPGSMQPKAPLWLFCLPFMVGLAAMGWERALRWGQRFLADPSW
                     RRRVGVGLWVSLAAGGVWLTVGRPLTSSAAFFPVFLGPRVVASSPAGLRLHDGSVVRA
                     FVPLIDELGVSETTVYSPSVPEDVWAGYQRWAGMRTKVRRVSDRERARLWVLDPGQEG
                     GNARLVGAVSRDAVMLVRLYDTR"
     gene            complement(5387..6262)
                     /locus_tag="PK710_10430"
     CDS             complement(5387..6262)
                     /locus_tag="PK710_10430"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT10178.1"
                     /translation="MIAMNMDDTRTGEMIGGRYLVGPRLGHGAHGEVYRARDTIDQQD
                     VAIKFLAHHLAHDTDYRLRLVREARIMSALKGLATVTIHGVIGADDGAPCVVMELMDG
                     KDLSDVLSERQKNEDYFSIDEVIRLFAPIVRTLDAAHAKGIVHRDLKPSNIFLCGGEV
                     DDSRIMDFGLAKAEDLSSITADQMLAGSPSYIAPEIWQRGAKEADSRSDIYSLAVVIF
                     ETLCFQVPVYRKELVQMLVAVTTPGNLASLHALRPSLPTHIDDWAQQALAIDPDNRFQ
                     TVVALWRALRSVLQH"
     gene            6426..6818
                     /locus_tag="PK710_10435"
     CDS             6426..6818
                     /locus_tag="PK710_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT10179.1"
                     /translation="MDNATNPRSKVSILIVDDNTMVLGLIKMVLEDMDFLVDTAGTAK
                     QAMACIDERRGLFDLVILDQQLPDSRGIDMMRRVKEQYPALRFMMVSGLMTEDVIQEI
                     KDAGGESVLGKPFDVAELMTAVEKALGE"
     gene            complement(6784..7947)
                     /gene="rlmN"
                     /locus_tag="PK710_10440"
     CDS             complement(6784..7947)
                     /gene="rlmN"
                     /locus_tag="PK710_10440"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOT10180.1"
                     /translation="MNAQRRFRHGDPVGPLARTPEQWKAWALTIGQRPFRGTQIFQWL
                     HARQQLDPDGMTDVPKAVRALLKTELEQHVPTLERVHEAADGTRKLVLRLTDHSAVES
                     VLIPMDAEDKGEDDTELEDEQDQACAQKQRVTECVSTQAGCAMGCVFCASGHPGLLRH
                     LTPDEIIAQVLIGRALLKPSQRLSNIVLMGMGEPLHNYDATVHAIQLMNCQEGLALSS
                     RRITISTVGLVPQIDQLGKDFQGRIGLAISLHAADDAVRTRLLPINRRYPLQMLMQAL
                     RRYPLPPRRRLTIEIALIDGVNDSEQMARSLVALLRGIPVKINLIPLNPVPHTALRPP
                     SEQRVEAFQEVLTRSGLSCFIRRRRGDDISAACGQLAGHPTSLIRQEPSPPRS"
     gene            complement(7944..8753)
                     /gene="mazG"
                     /locus_tag="PK710_10445"
     CDS             complement(7944..8753)
                     /gene="mazG"
                     /locus_tag="PK710_10445"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOT10181.1"
                     /translation="MPPFLEPRNGLGGNLPRLLLIMRKLLGPQGCPWDQQQTLSSLRR
                     YVLEEACEVIDAIDENDPDALREELGDLLLQIAFQSELSRNKGDFDIDRVIDGICDKL
                     VRRHPHIFSDTKVKNAEQVHQNWERIKATEKQRMSILDGIPTSLPALARAQRLGEKSA
                     RVGFDWPHLEGCREKVNEELSELDDALAAHDRESAAEELGDLLFALANLARHIGVDAE
                     MALRETNEKFVRRFQQVEQRVRVDHGGFESGPIDIDTLERYWQQAKQKEPQ"
     gene            9054..>10032
                     /locus_tag="PK710_10450"
     CDS             9054..>10032
                     /locus_tag="PK710_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOT10182.1"
                     /translation="MSDQDSTPTSNILQRVPTSILDEMRSSYLDYAMSVIIGRAIPDV
                     RDGLKPVHRRIMYSMHEQKIGPSGPHKKCARVVGDVIAKYHPHGDAAVYDALVRMAQD
                     FRMRHPLVDGQGNFGSIDGDPPAAYRYTECRLERISTELLLDLEKDTVDLAPNFDSSE
                     SEPVVLPAKFPNLLVNGSEGIAVGMATNIPPHNLGEVVTATIELIRNPDLSIEQLMQY
                     IPGPDFPTGGLIYGRTGIEQAYQTGRGSIIMRAKTDFEQIPGKDREQIVVTEIPFQVN
                     KARLHAKIGELIRERKIEGVAEVRDESDREGMRLVIELKKDAHGPVVLNQ"
BASE COUNT         1986 a         2401 c         3286 g         2359 t
ORIGIN      
        1 ggaaggaacc tttgctcgat cgtgcaagcg cggggggtgc tgcctgtttc tcaagtattt
       61 tcaacagctt tggatatttt ggacgccctc gaggacatgc atcggcaagg gattgttcat
      121 ggcgatatcc ttccggagtg cattctgatt ggggccacgg aagagggcga ggtgcgggcc
      181 aaagtggtca accacgtgct ggggcgtctt gcatccgtgg cgataccgag gctggcgtgc
      241 ggcgggactg ctagtgggac ttcgcgctac atggctcccg agttggcgat gggcgagccg
      301 ccttcggtgt tcagcgatat ttattcgttc ggtgctgtcc tttatcaaat gcttacggga
      361 attccgcccc gtcgttcatc ctgttgtctt ctgatggaac gccagtggca aaagcccgtg
      421 gttccgccat cgatggtagc gaaagaccgt accatttctt cggcggtgga cgacgtgctg
      481 cttcgcgctt tggacagaga gccatcgcga agattttcgt cggcctcgga gatgcggcaa
      541 gtgctgtggg aggcgaagaa cgaatcgccg gcggttattt cgacaccgac gattacggtt
      601 ccatccaacg ggcatcgctt gtttggtcgt cagcaagccc tggacatcgt gcatacgatt
      661 gcgttgatgc cggtggcgta tgggactgag gatcaggggc atccccgcgg agcggtggcg
      721 gtggtcgtgg gcagcgcggg tgtgggaaaa acggctttgc tacgcgctgt cagggaccga
      781 ttggaaggaa gcccggtggc cgtgttgcgc gtggatggtc gacgtgccct cacacgtccc
      841 ctcgagcctt tcattacgtt ggtgcaggac gttttggggg tgcgtcgtgg tgatgagcca
      901 aagactatcg acgaagtgag aaaggcgctc gtgttgcgct ttggaatcga agaacaagac
      961 tgccttcgtt tgctcgagcg tgtgacggat cgtgaatcgt cgttgagtct tacccccgac
     1021 atcatcgaac gggagcaggt tcgttcgctt cgggcttttt tgggtcgaat gcttgcctca
     1081 cggccgacag tattggtggt ggaagacgcg gatgcgatgg atacggcatc gctcgagttg
     1141 acgcgggact tgatggaagc atcgtcgacg cttgcgttat cggttcttgt ttcgtgccgg
     1201 agcgatcctt atccggattg ggatgctggg tacgtgacgc gacttcatct cgatgctttg
     1261 gatgagatgg catcgaagtc gatgttggag catcggacga tgggaatggt agttccgtcg
     1321 gatgcgatgg cgatggcggt tcaatggtcc aagggggtgc cgttgttgtt ggagcttacg
     1381 agccgggcga tgcgtcaacg tgagtttttg gtggagcctg atggtacttt caaagcgcca
     1441 tctacctcgc aggatttatt tggagggttg agacaactcg tatcgatagc gttgcgggat
     1501 cgcccggtgt gtgtgcggca gtggatattc gtcgtggcga tagcgggggg tgctccgatc
     1561 gaattgctgg aagcttatga gtcgtcgcaa ggggcgatcg aagagacgat cggggcatgt
     1621 ttggaaacgg gatgggtgag ttattcggat gaggtgatgg agtttgataa cgagggggtc
     1681 cgagacgtcg ttcgttcgat gattccggaa gggacgaagc ggcagatgca cgcgtttgtg
     1741 gcgaagtggt tacaacagac gacaccggca cgggcacctg gggaggtgat tgcttcgcat
     1801 cttgtgatgg ggggagatgt attgggagcg gcgcaagcgt atgaaaaaga agcgatagcc
     1861 ttgtcttcgc gcgaggagca tgtagccgcg acggtgatat ggggtcgagc ggcgcaggca
     1921 tggcagatgc ttcgggatga gcaagcgtta tgtcgcgtgg cgatagcgcg gacggaagcg
     1981 tttttggcgg cgggaaatgg caaagccgcc acggaggagc tttcggcgtt ggagaggttc
     2041 gagccggcag gattggatgg tttacgcgcg cgggcgatgg ccgcggtggc aagtagccag
     2101 agcaattggg atcttgcgat tcaatcgttg cttcgcgcca gtgcgttggc tgtggacaat
     2161 ctcgaccaac aggcttggtt cgatgtggaa ttttgtctcg gggaattgct gtatcgtcgg
     2221 ggtgaattgg gagctgcgga atcccacgca accacctgtt tggatttggc catctcgatt
     2281 gccgacagcc ggggcgagcg cgccacgctg gtggatggtg cgcgaatcgc gcaagcggcg
     2341 acctttttat ccaaactcag gatagaaacg aaccgcatgg ataaggcgcg gaatgctttg
     2401 gccatgtcgt tggaacatac ggccatcctg ggggatgaag catccgcttc gcgtttgctt
     2461 gcgaatctgg cttatgcctg tgaaccggaa gatttggagg cctcgctttc gtatgcgaaa
     2521 cgtgcgctcg agttggcgct tcgcgccggt gatcgcatgg cagcagcccg tatcgcgatc
     2581 aacttgggcg cctatcaagc gcaatcccat gcgtttgaag atgcgcttgc gtcgtatgcg
     2641 atggcgaaaa aactggcgct ggctgtcggc tgggcccgag gcgtgcggtt ggccaagtcc
     2701 gcggccgaaa aaatccattc cacgtcatct tgagacaaga cgcgggacat ggtgattcgg
     2761 gttttcgaat caataatctc cgaacaggtc ctgaacgcgg acatccaagg cggcggcgat
     2821 tttatataac gaggacaaag acgccgagga ttccgcgcgc tcgatctgcg acagcaaaga
     2881 aacgctcaac cctgttcgtc gggacatctg tttgagcgtg agttcttttt ccttacgaag
     2941 gcttcggatg ctttcgccga tcactttgtg gagctgctcc tcgggggtgc ggctcaagcc
     3001 tttttttcgc atgacccgat ccagcacctc acggaattca tccacgttga aaggcttttt
     3061 tacgtaatcc acggcatcga gtttcatcga agccaccgcg gtctcgaggt tgggatatcc
     3121 ggtgaaaatg accacggcaa cgtctttgtc gatctttcgg attcgcccca atagttcgat
     3181 gccatccatg ttcggcatca ttaaatccag aattatcaag tgataatgtc cctggcggag
     3241 ttcgtcttcc accagaaacg gatcattgat ggtcttgacc tcgaatcgat cgcgttcgag
     3301 cagcgtctgc atatagtcac agatcgcttt atcatcatcg acaatgagaa ttctgaccgt
     3361 agggagttcg atgggcacga caacctccgt gattgtaggc cacccaatgc ttggttttca
     3421 ctcatagcac aatttatggt cggctcctac gcttgttcgc aggaaagtat gaccgaaatt
     3481 cccagaatcg acgcctgtgg ggctttgaga aaaatgtgtt atttcaataa cttagccgat
     3541 aatggtttgc gtaggagcgg aaaacgcgag ggcgtgatag tttgcctcgg cgttttttcc
     3601 cgaaccttat tctcgaacca tcgacgagta gccgcgcagt catgatccgg tttgacaagc
     3661 attccccatg gctcgaccgt gccatagttc tggggttgta cctccttgcg gtagccgtgt
     3721 tggcgtatca cgcgcgtcaa gcgggaatag gggccgatga gtcggcctat ctcgacgctg
     3781 gccgacgggt tggggaatgg ttcgatcgga ttgggtccgc ggagtcgtcg ttttttaccc
     3841 aagcggatcg agatgctgtt tgggcgcccg tccatgcggt ggtgaatgca tcgaccttac
     3901 tatccgcgtt ggtgtggcac gtggctttca gcggtttcga tgtattgggc gagtgtgtgt
     3961 cgttgcgatt gggcacggtg cttctgaacg ctttctccgt acctttgctg tatgcgcttc
     4021 tcactcctgg tttggggaga aaggtgggag gtttgtcggt gttgttgtgg atgctcgtcg
     4081 ttcgcgcgca acatgaggca tcgttggcaa atccgggcgt ggtggcgatg acgggaagcc
     4141 ttgccgtgct ggtggctcat gggagagcaa gaagcgcggg cagggaaaac cgcatgggga
     4201 aaagcgcggc gtggatggcg gttgcgggat tgttgctggg cgttggcgtt gcggtttcac
     4261 atgccacgat cgtggtattg ctcgttcttt tcaccaattc gctttggctt cagcgaaaaa
     4321 ggcttcgaga aatgctgcgc gggggggacg ttccggttcc atcgtccatg gtatttttcg
     4381 ctattttcgc accgttggcc tatggcttgg caacgccgtg gttgtggcat gatacggcag
     4441 cgcggattcg gcccatgctt gttcgggcgt tctcgccagg gacgcaggcg caggggatcg
     4501 ggcataccgc gttgtcgctc gtgctatcca caccgatggt gatcgtgctt ggggcgctgg
     4561 tagggatcgc ggtaacgata gggccttccc ggtggcgagg gtggggcaaa aaggagacga
     4621 caggggacga tgagactccc gcgttgctca tcgttgggct cctgacaggc cttgggtgga
     4681 catggttatt gccggggtcg atgcaaccga aggcgccgtt atggctgttt tgtcttccgt
     4741 tcatggttgg gctagcggcg atggggtggg agcgtgcgct gcggtggggt caacgttttc
     4801 ttgcggaccc aagctggcgg cgcagggtcg gcgtggggtt gtgggtaagc cttgcggcag
     4861 ggggagtgtg gctgacggtg gggcggccgt tgacaagctc cgcggccttt ttcccggtct
     4921 tccttggacc tagagtcgtg gcttcttccc ccgccggact gaggttgcac gacggttcgg
     4981 tcgtgcgcgc gttcgtgcct ctgatcgatg agcttggggt ttccgaaacg acggtgtact
     5041 cgccttccgt accggaagac gtgtgggcgg ggtatcagcg ctgggcagga atgcgcacga
     5101 aggttcgaag ggtgtcggac cgcgagcgtg cgcggctgtg ggtgctcgac cctgggcaag
     5161 aggggggaaa cgcaaggctc gtgggtgccg tttcccgcga tgcggtgatg ctcgtgcgat
     5221 tatacgatac gcgttgaggc cagggcgcgg aacacgttga taaccatatg aattaattga
     5281 tcattaattg attgtggcgg cgatggggtt cgctcgtatg ctccgaggcc ctacggggga
     5341 aagcgcgttg gatagtgatt gggcaaccgg gtagtatcga ttgggttcag tgttgaagga
     5401 cggaccgcag ggcgcgccac agggcgacga cggtttggaa gcggttgtcc gggtcgatgg
     5461 cgagggcttg ttgcgcccaa tcatcgatat gggtgggcaa ggaggggcgc aaggcatgga
     5521 ggctggccaa gttgccgggg gtggtgacgg ccacgagcat ctgcacgagt tctttgcgat
     5581 aaacggggac ttggaagcag agggtttcga aaatcaccac ggcgagcgag tagatatccg
     5641 accgggagtc ggcttctttg gcgcctcgtt gccagatttc gggtgcgatg taggaagggg
     5701 agccggcgag catctggtcg gcggtgatgg aggacaagtc ctctgctttt gccagtccga
     5761 agtccatgat tcgtgaatcg tcgacttcgc cgccgcataa aaaaatattc gaaggtttca
     5821 aatcacggtg gacgatacct tttgcgtggg ccgcatccag ggttcgcacg atgggagcaa
     5881 acagtcttat gacctcatcg atcgaaaaat aatcttcgtt tttctgtctt tctgacaata
     5941 catcggaaag atctttacca tccatgagtt ccatgaccac gcagggggca ccgtcatcgg
     6001 caccgatgac gccgtggatt gtcacggtgg cgaggccttt caaggccgac atgatgcgtg
     6061 cttcgcgaac gagtcgaagc cgataatccg tatcatgggc gagatggtgg gccaggaatt
     6121 tgatggcgac gtcttgttgg tcgatggtgt cgcgagcgcg atagacttcg ccatgggcgc
     6181 catgtccgag gcgtggtccc acgagatagc gtcccccgat catttctccc gttcgtgtgt
     6241 catccatatt catggcgatc atgattctat cgacttattg ggtgagaagt gaaggaaccg
     6301 caaagcgcgt acgtatttgg aataggggag gaaaataagc tgtctaaaat cgcccatggg
     6361 tggcgtggtg cggtataaga caaaaggtgg gcttcggtgg acaccgtgtg ggcggttttt
     6421 gaaccatgga taatgcaacc aatcctaggt cgaaggtcag catactcatc gtggatgaca
     6481 acacgatggt gttgggactg atcaaaatgg tgcttgaaga catggatttt ctggtcgata
     6541 cggcgggcac cgcgaagcaa gccatggctt gtatcgacga gcggcgtgga ttgttcgatc
     6601 tggtgattct cgatcaacag cttcctgaca gccggggcat cgatatgatg cggcgtgtga
     6661 aagagcaata cccggctttg cgcttcatga tggtgagcgg attgatgacg gaagatgtga
     6721 tacaggaaat caaggatgcg ggaggggaat ccgtgttggg caagcctttc gatgtcgccg
     6781 aactcatgac cgcggtggag aaggctcttg gcgaatgagt gaggttggat ggcccgccaa
     6841 ctgaccgcag gcggcgctga tatcatcgcc ccgacgtcgt cgaatgaagc aggatagacc
     6901 ggatcgggtt agcacttctt gaaacgcttc gacgcgttgc tccgaaggag gacgcaaggc
     6961 agtgtggggc acgggattga gggggatcaa gttgattttg acgggaattc cacgaagcaa
     7021 ggcgaccagc gatcgtgcca tctgctcgct atcgttgacg ccgtcgatga gagcgatttc
     7081 gattgtcaac cggcgacgtg gaggcaacgg atatcgtcga agggcttgca tcaacatctg
     7141 cagcgggtag cgtcggttga tgggaagaag gcgtgtgcga acagcgtcgt cggcggcatg
     7201 aagcgagatg gctagtccga ttcgtccttg aaagtctttg cccaattggt cgatttgcgg
     7261 cacgagcccc acggtgctga tggtgatacg tcgggaagac agggcgagtc cttcctggca
     7321 attcatgagt tgaatggcgt gaacggtagc gtcgtagttg tgcaatggct cgcccatgcc
     7381 catgagcacg atattggaga gacgttggga aggcttgagc aaggctcggc caatcagcac
     7441 ttgggcgatg atttcatcgg gagtgaggtg acggagcaat cctggatgtc cgctggcaca
     7501 gaacacgcac cccatggcgc agcctgcttg ggtagaaacg cattcggtga cccgctgttt
     7561 ttgggcgcaa gcctggtctt gttcgtcttc gagttcagtg tcgtcctctc ccttatcctc
     7621 ggcgtccatg gggatgagaa ccgattccac ggcggaatga tccgtcaaac gcaagacgag
     7681 ttttcgggtt ccatcggcgg cttcgtgaac tcgttcgagg gtgggaacat gctgttcgag
     7741 ttcggttttc aacagggcac gcacagcttt gggaacgtcc gtcatgccgt cggggtcgag
     7801 ttgttgtctg gcatgcaacc actgaaaaat ttgtgttcct cgaaaaggcc gctgaccgat
     7861 ggttagtgcc cacgctttcc actgctctgg tgttcgagcc aaaggcccaa ccggatcgcc
     7921 gtggcgaaag cgacgttgtg cgttcactgt ggctcctttt gctttgcctg ttgccaataa
     7981 cgttcgaggg tatcgatatc gatcgggccc gattcgaagc cgccgtggtc gacccgaacg
     8041 cgctgttcga cttgttggaa tcgtcgaacg aatttttcgt tggtttctcg cagggccatt
     8101 tccgcatcga ctccgatatg gcgtgcgaga ttggcaaggg cgaacagcag gtcgccgagt
     8161 tcctccgccg ccgactccct atcgtgggca gccaaggcat catcgagttc ggacagttct
     8221 tcgttgactt tctcgcgaca gccttcgaga tgcggccaat cgaaccccac ccgtgcggat
     8281 ttttcgccga gacgttgtgc gcgagcaagc gcgggcagac tcgtgggaat cccgtcgagg
     8341 atgctcattc gttgtttttc cgtcgctttg atgcgctccc aattctgatg gacttgctcg
     8401 gcgtttttca ccttggtgtc gctgaaaata tggggatgtc gtcgaacgag cttgtcacag
     8461 atgccgtcga tcaccctatc gatgtcaaaa tcccctttat ttctggatag ttctgactgg
     8521 aaggcaattt gaagcaacaa gtcaccgagt tcttcgcgca gggcgtcggg atcgttttca
     8581 tcgatggcgt cgatcacctc acaagcttct tcgagcacgt atcttctaag ggaagagagg
     8641 gtttgctgtt ggtcccaagg gcagccttgg ggcccgagga gctttcgcat gatcaggagc
     8701 aaacgaggaa ggtttccgcc taatccgttt cgaggttcga gaaaaggggg catggagggt
     8761 gccatagcat ggtccgtgag gtgttctgca gcgcgaaacg agagcgagga tcgaaagaac
     8821 cggaataccg accgatgggg ggtaacggag gtggagcagg aatctctcat gatttcgcgc
     8881 tattggtttg gcaaggatcg gttgggcaga gtcgagctgc gaagactgcc attcacgttg
     8941 caggaagctc ttctctcgac tacttcaccg acagcctccg cggaggaccc acggttttgg
     9001 ggttccccta ggtctgagga gacctacctg aggacggccg gagaagccgc acgatgtccg
     9061 atcaggattc aacgcccacc tccaatatat tgcaacgtgt tcccaccagc atcctcgacg
     9121 agatgcgatc gtcgtatctc gactatgcga tgagcgtcat catcggtcgc gcgatccctg
     9181 acgtgcgcga cggtctcaag ccggtgcatc gtcggatcat gtattcgatg cacgaacaga
     9241 agattgggcc ttcgggaccg cataagaagt gtgcgcgcgt ggtgggtgat gtgattgcga
     9301 agtatcaccc gcatggtgat gcggcggtat acgacgcgtt ggtgcgcatg gcgcaggatt
     9361 ttcgcatgcg tcatcccttg gtggatgggc agggaaactt tggttcgatc gacggcgatc
     9421 ctcctgcggc ttatcgatat acggaatgtc ggttggagcg catatcgacg gagttgctgc
     9481 tggatctgga gaaggacacc gtggatttgg cgccgaactt cgactcctcg gagtccgagc
     9541 cggtggtgtt acccgcgaag tttccgaatt tgctcgtcaa cggatcggag gggattgcgg
     9601 tcgggatggc caccaatatt ccaccgcaca atcttgggga ggtcgtgact gcgacgatcg
     9661 agttgattcg caatcctgac ttatcgatcg aacaactcat gcagtacatt ccgggtcctg
     9721 attttcccac gggaggactc atctacgggc gcacgggtat cgaacaagcc tatcagaccg
     9781 gccgtggttc gatcatcatg cgtgccaaga cggacttcga gcagataccg ggcaaggacc
     9841 gcgagcaaat cgtggttacg gagattccat ttcaggtcaa caaagcgcgt ctccacgcca
     9901 agatcggtga actgatacgc gagcgaaaga tcgaaggagt tgccgaagtt cgagacgaaa
     9961 gcgatcgaga agggatgagg ctcgtcatcg aactgaagaa agatgctcat gggcctgtgg
    10021 tgctcaatca gt
//
LOCUS       DAOSOR010000159        10024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6722_length_10024_cov_4.198114, whole genome shotgun sequence.
ACCESSION   DAOSOR010000159 DAOSOR010000000
VERSION     DAOSOR010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 10024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10024
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6722_length_10024_cov_4.198114"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..618)
                     /locus_tag="PK710_10455"
     CDS             complement(64..618)
                     /locus_tag="PK710_10455"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HOT10183.1"
                     /translation="MAVPLRGEHAWYATALFSAVGSPYRSLGDLQGARVAWVDPKSMG
                     GHVVMKAWLRSQGIAVEDCFAEQQYFGGHEQVVAAVLEGRADVGATFAHPDPQGDGLV
                     SAGWGDASVQVIAVAGRIPSDVLAASPTLSSSQVGAVRDALTQDMNAKLRGAALALFE
                     ADQFAPADEAAMKSLAELVVHWDT"
     gene            complement(513..896)
                     /locus_tag="PK710_10460"
     CDS             complement(513..896)
                     /locus_tag="PK710_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10184.1"
                     /translation="MTQSDTLTVGMVLPTPGPGAKRPAVAVASVRLKLAVFCGALEDD
                     LSISKTDSVRVLPKTFDAYAPLLEAVSSRQVALAWLPPLLASRQVMSLPWPYLCEGNM
                     LGTQPRYSPRSARHTDRWGIYKARG"
     gene            1189..1560
                     /locus_tag="PK710_10465"
     CDS             1189..1560
                     /locus_tag="PK710_10465"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HOT10185.1"
                     /translation="MRSALEIEVQDVPQAASVRSWILRRAKKLAARHPDITKLRVAVN
                     TPHRRQSKGRKFRVQIDIGVPGTDIVAHNLPTAKCEQLYPALHEAFHRSERQLDELRE
                     TRRVRDRRRARKANPFAPRQS"
     gene            1887..3008
                     /locus_tag="PK710_10470"
     CDS             1887..3008
                     /locus_tag="PK710_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10186.1"
                     /translation="MAFIVGLWVGIHRVSWLGPALADAARTVVGPSAVAKVEDVVFGI
                     EDGWNRFWRSEQTPVVYWAAPSFSTADVSSSSLPPVVPSEPRFRPCDVGPMDTKTSLA
                     NDGVWIAIELSGREHEPCLFKTLLHPDGHRPWSTVALVAVDLASTGLHLVAGVQEPKA
                     TNPKAKTVVRSGLVPKADHGRLIAAFNGGFKAEHGALGMQIDALTFLPAQPWGCTISR
                     TSEDGLLIGSQNKIGDRLLGARWWRQTPPCLVEEGILAKGVLAEGNINWGKAVHGDTI
                     IRRSAIGIDERGTTLFVGIGEATSAGSMARAMRHAGAHTVAQLDVNGPFPKFLLFERG
                     GGQGRLIARPLCGGFPYSEQDYVEKPSARDFFYLTKRGL"
     gene            complement(3017..3712)
                     /locus_tag="PK710_10475"
     CDS             complement(3017..3712)
                     /locus_tag="PK710_10475"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10187.1"
                     /translation="MPNVDVLLAAPHAPELEGLESILGPTMSATVQGIHVVAKTVGLG
                     LPGVAAGTTQRLERYRPRAVILLSTCAAYGHRGLTPGQVVVAERLVIADAAVLKGWAS
                     FPEPMVGEIHTHAGLVGGLTTAGTRRVSAASSCSLTSDVQLAADIVARLGCDVEHNEV
                     FGVALACAAYQIPFAAVFGVAYEIGPKSQETWRTTHRTATMGAASLIAAWLRAGAVGV
                     PHGTPPESPITLL"
     gene            3936..6305
                     /locus_tag="PK710_10480"
     CDS             3936..6305
                     /locus_tag="PK710_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HOT10188.1"
                     /translation="MNQPFRHVAVLGAGVMGAAIAAHLANAGVRVLLLDIVPPNLSQD
                     DKKDPKKRNAIAVGGLERAVKAKPALFFHPSCASLVRTGNLEDDIHKLADCDLVIEAV
                     PELIDIKRSLFERVEKAIRPGTVVSSNTSGLPIRDLLEGRGPEFRKNFIVTHFFNPVR
                     YMKLLELVRGPETLDSVYERIVAFGQDVLGKGIVVGRDTPNFVGNRIGVYAMMVAIHQ
                     MLESGLAPEEVDAVAGPPMGRPKSAAFRTADLVGLDTFVHVADNCHRLLADDPQRDVF
                     LVPPFIRTMVERGQIGNKAKGGFYKKDKAKNVLTLDPATGEYRDKLAKSDVLGFCKSL
                     KDIDEVGARVRKLMSDTGPAGDYAWKVAAKTLLYAAGLVGEICDDVTAIDDAMRWGYN
                     WELGPFEMWDALGFARTVDRMQKDGFEIPASIRKMKDDGVKGFYDGDKIYDLVAGKQI
                     ARKTDPRTLPFSIVRGEKAIEKLEGASLWDTGDGVLAVTFHTKANSIDHDVIAMIEKG
                     VTRAENEARALVLFNEGEHFCVGANLMLIVTAAINKKYEDIRQVAAALQAAGQRMKYA
                     KVPVVTAPFSVTVGGGLELCMAAGHVQAAAETYCGLVEVGVGLIPAGGGCTNAMWRAL
                     ENVPEGAPADIYNLTTQVFKNIALAKVCTSALEGQRVGYFRSGDGITFDKARLLHDAR
                     KYAIGLAERGYHPPIPRSHKLFGESGIATFRMMVSALVAGGQATAHDGVVATRLAEVL
                     CGGVDGAAAPVTEERMLELEVEAFLSLCGEEKSIARMQAMLTTNKPLRN"
     gene            6432..7607
                     /locus_tag="PK710_10485"
     CDS             6432..7607
                     /locus_tag="PK710_10485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOT10189.1"
                     /translation="MREVVIAASYRSACGRAHKGTLALTRPDDLLAQVIAGVLERVPQ
                     IKPEMIEDVVIGCAMPEAEQGMNVARCSAMLAGLPQHVPAITTNRFCSSGLQAIATAA
                     HQIAFGEMDIALAGGVESMTMVPMGGNKLTASAAIMDRVAAVYTPMGITAENVAKRFE
                     VSRAVQDEFAYHSQIKAATASQNGLFRDEIIPVKATRYATKNGVRVRDDVVFDTEELP
                     RMDTTLEGLAALRPAFSATGSVTAGNSSPLTDGAAISLLTTKERAQQLGLEILAYFRG
                     FQVVGVDPEIMGVGPAYAVPKLLTRYNLTLDDIDLVEMNEAFASQSVYCLRQLGLPAE
                     KVNVNGGAIALGHPLGATGAKLTATLLHEMKRRNAKRGIVTMCIGGGMGAAGLFERP"
     gene            7751..>10024
                     /locus_tag="PK710_10490"
     CDS             7751..>10024
                     /locus_tag="PK710_10490"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT10190.1"
                     /translation="MPPSLPSPHPFRPRKIGRYLLHREIAFGGMATVHIGRLLGQVGF
                     SRTVAIKRMHDHCARDPEFVSMFIDEARLAARIRHPHVVPTLDVVALEGELFLVMEFV
                     QGETLARLIRRSVKARIPISLQVAAAVISGVLEGLHAAHEAIGEHGEFLGIVHRDVST
                     QNIMVGTDGVSRVLDFGVAKAINRIQTTRDGQIKGKIEYMAPEQIRGEAVDRRTDIYS
                     VGCCLWEMLVGRRLYVADNQVTLWGMVLQGNPPKPSSVNPNVTPELDAITMRGLEADP
                     SKRYETAEQMAIELEQNVGIATPREVGRWVQSLVSKKMQALDEVLAELDSVSKSISVF
                     ASDQTSAEHTALSAEQPAVPVPSPVQRHSSPNHPVVVPTPIPDADQPSQPRRPQASAP
                     VINVPRPPIPSVPEPRPPRRSSQPRISRLSPVPPPRQHQNKDNISNKLQPGGSSTDTP
                     PPTAMAPSNSESSKDAAALLPRTPRPSDSYLRAENESPADSAIAAQTAKSPDRTSAIS
                     FPLPSAGGGSPMLPTDFSEVAAEASEQHTPPTSEQGASTDPASAEGASSPPSGAEPAD
                     TVQAIASDSPGVAPLLDPSPVVSSDLFSGSTQDPFGFTRKKSKAKWWGAGGIGLGLAV
                     IAVLVGTWKGAQTTEPSAPSPDGSVSSSMPRVPDSVQVAEQASPVASASGSETQDAAP
                     ATVRLRLELSPRTAKVAVDGVSVSESEIRLPLSDKPVKIVAEAAGFSPMERAFVPSKD
                     GVVVFELKRLPSTVRPTT"
BASE COUNT         2071 a         2802 c         3047 g         2104 t
ORIGIN      
        1 cgtatcggcg cccgctcctt gtgcgcactg attcgaacgc gcaaaaatga ttcgggcaac
       61 cgactacgta tcccaatgaa cgacgagttc ggcgagggac ttcatcgcgg cttcatcggc
      121 aggggcgaat tggtcggctt cgaacaacgc cagggcggct ccacgcagtt tggcattcat
      181 atcctgcgtc aatgcatccc gtaccgcacc gacttgactc gaggaaagcg tagggcttgc
      241 ggcaaggaca tcggaaggaa tgcgtcccgc gacggcgatg acttgcacgg aagcgtcacc
      301 ccatccggcg ctgacaagac catcgccttg ggggtcagga tgcgcaaatg tagcgccaac
      361 gtccgcgcgg ccttcgagca cggctgccac gacttgttca tgccctccaa aatattgttg
      421 ctccgcaaag caatcttcca ctgcgatgcc ttgtgaacgg agccacgctt tcatcacgac
      481 atgaccaccc atgctcttgg gatcgaccca ggctacccgc gcgccttgta aatcccccag
      541 cgatcggtat ggcgagccga ccgcggagaa taacgcggtt gcgtaccaag catgttcccc
      601 tcgcagaggt acggccacgg gagtgacatg acctgccgcg atgccaacaa cggcggaagc
      661 caggcaagcg ccacttgccg agaagaaaca gcctctagca gcggcgcata cgcatcgaat
      721 gtcttcggaa gaacccgaac cgaatccgtt ttcgaaatcg atagatcatc ttccaacgca
      781 ccacagaaca cggccagttt gagtcggacc gacgcgacag ccacggctgg ccgcttggca
      841 ccaggaccgg gggtggggag aaccatgccc acggtaagag tatcggattg tgtcatgaaa
      901 gaacgatggt cttggtggac gacggggtca acgattcacc gtgaaaaccg aaggttatgg
      961 gggcggcggg acggcaaggg gcgctcgcct gctcggttcc gtcggatatc cacgtttcta
     1021 tttttttccg gctccaagcg gggaattggt aaaaactgtg taattacaaa tagttacaat
     1081 tattttcgac ctgggtcgcg atctgtggcg gttactggaa tgaaatcggt ccgatgcggg
     1141 ttgacctttt tatcaccggg gggttcccca attcaccagg agaacggcat gaggtctgct
     1201 ctcgagatcg aggttcagga cgtcccgcaa gcggcttccg ttcgaagctg gatactgcgt
     1261 cgcgcgaaaa aactcgctgc ccgtcatccc gacatcacca aactgcgtgt cgcggtcaac
     1321 acgccccatc gacgtcaatc caaagggcga aagttccgcg tgcagatcga tattggcgta
     1381 cccggaaccg atatcgtggc tcataacctt cccacggcca aatgcgagca actatatccc
     1441 gcgctgcacg aagcgtttca tcgatccgag cgccagctcg acgagttgcg tgagacgcgg
     1501 cgcgttcgtg accgacggcg cgccaggaag gcaaaccctt tcgcaccacg gcagtcctag
     1561 gattcgctgg caaaaagtac gtagcaagcc cacggttgac ggtggcttga cgatctggtc
     1621 ccatcgcgtc acgctttttg ggagattgta cggatgggtg atcagtcgga atctcaggca
     1681 ccagagtccg gaaaagggct cgtcaacgtt caggagcgca gcggcgacta cgatggtacg
     1741 tgcttgcgcc cccttgggct gtgcgaactg ggcggcgcct gcgatgtttg ctggtaccga
     1801 cccaatcatc cacggtttca aaatcataaa gacgagtgag gcgtcgacgg ctgaccaggg
     1861 ttgtgttcgg tgggatagcg cttttgatgg cgtttatcgt agggttgtgg gtggggatcc
     1921 atcgcgtatc atggctaggt ccggcccttg cggatgccgc gaggaccgtg gtgggacctt
     1981 ccgccgtggc caaggtggaa gacgttgttt tcggcatcga ggatggctgg aatcgctttt
     2041 ggcgttctga gcagacaccc gtggtgtatt gggctgcgcc ttccttttcg accgccgatg
     2101 tctcctcgtc gtctttgcct cccgtggtgc cttcggaacc tcgttttcgt ccttgtgacg
     2161 tggggccgat ggatacgaaa acctcgcttg caaacgatgg ggtatggatt gcgatcgagt
     2221 tgtcgggtcg tgagcacgag ccctgtcttt tcaagacgtt attacatccg gacgggcatc
     2281 gcccttggtc cacggtagcg ctggtggctg tggatctggc ttcgacaggg ctgcacctgg
     2341 tggcgggggt acaggagccg aaggcgacga acccgaaagc aaaaacggtc gttcgaagcg
     2401 ggttggtgcc gaaggcggat catggccggt tgattgcggc attcaatggt ggattcaaag
     2461 ccgaacatgg agcgctgggg atgcagatcg atgcgctcac ctttcttcct gcacaaccct
     2521 ggggttgcac gatatcgcgc accagcgaag atggtttgct gattgggtcg cagaacaaaa
     2581 tcggggaccg attgcttgga gcgcgatggt ggcgacagac gcctccttgt ttggtggaag
     2641 aaggcatatt agcaaaaggc gtactggcag aaggaaatat caattgggga aaagcagtac
     2701 acggtgacac gatcatccgt cgctccgcca tcggcatcga tgagcgaggc accacgctat
     2761 tcgtggggat tggagaggcg acatccgcgg gatccatggc ccgagcgatg cggcacgcgg
     2821 gggctcatac cgtggcgcaa ctcgacgtca atggtccatt tccgaagttc ttgttgttcg
     2881 agcgaggcgg tgggcaaggg cggctcatcg cgcggccgtt atgcggtgga tttccgtatt
     2941 cggagcagga ttacgtggaa aaaccttcgg ctcgggactt tttttatctg acgaaacgtg
     3001 gcctctgaac tgcccgtcaa agaagggtga tgggactctc gggcggcgtt ccgtgcggta
     3061 cgcccaccgc tcctgcccga agccatgcag caatcagcga tgcggccccc atcgttgccg
     3121 tccgatgggt tgttcgccac gtttcctgcg acttgggacc gatttcataa gccacaccga
     3181 aaaccgcggc gaaagggatc tggtaggcag cgcacgccaa agccacgcca aacacttcgt
     3241 tgtgttcgac gtcgcagccc aagcgagcca cgatgtcagc ggcaagctgg acgtcgcttg
     3301 tgagagagca agaggaggcg gccgatacgc gacgtgttcc cgcggtcgtc aagcctccaa
     3361 ccaatccggc atgggtatgt atttctccta ccattggttc cggaaaactc gcccagcctt
     3421 tgagcactgc ggcatcggcg atgacgagtc gttctgcgac gacgacctgt ccgggggtca
     3481 gaccacgatg gccatatgcc gcgcaggtgc tcaataagat gactgctcga gggcgatagc
     3541 gttcgagtcg ttgcgtggtg cctgccgcaa cgccgggcaa acccaagccc acggttttgg
     3601 cgacgacatg gattccctgc acggtggcgg acatggtggg accgaggatc gattcgagtc
     3661 cttccagttc gggggcgtgc ggggcggcta ggagcacatc gacgttaggc acaatcccat
     3721 gattactcta ctgcgcatcg aagatcagca gggtagatga gcttgcgtgt gtagcgcagt
     3781 gcgttgcgat taaccgacga gcagattagt gaatcgtctc aaatggttca cgttattcga
     3841 aattcatcgg tcattgccaa ggaacaaaat ttcgcgcaat ttagacgcgt cgcgtcgacg
     3901 gggcgcgatg agacaccaaa ccagagaggg ttcggatgaa tcaacctttt cgacatgttg
     3961 cggtgctagg ggccggcgtc atgggagcgg ccattgccgc gcaccttgcg aacgcggggg
     4021 tgcgtgtgtt gttgctcgac atcgtgccgc ccaacctcag ccaggacgat aaaaaggatc
     4081 cgaaaaagcg caatgcgata gctgtcggtg gattggagcg tgctgtcaaa gccaaaccag
     4141 ccctcttttt tcacccttcg tgtgcatcgc tcgttcgcac gggcaatctc gaggatgaca
     4201 ttcataaatt ggcggattgt gatcttgtca tcgaggcggt tcctgaactc atcgatatca
     4261 agcggtcgtt gttcgagcga gtcgagaagg ccatccggcc tgggacggtt gtatcgagca
     4321 acacatcggg tcttcccatt cgggatttgc tcgagggtcg gggtccggag ttccgcaaga
     4381 acttcatcgt gacgcatttc ttcaatccgg ttcgctacat gaaattgctc gaactggtgc
     4441 gtggtccgga aacgctcgac agcgtgtacg agcgtatcgt ggcgtttggg caagacgttc
     4501 ttggcaaggg gattgtggta gggcgcgaca cgcccaactt cgtgggcaac cgtattgggg
     4561 tgtacgccat gatggtggcg attcaccaga tgttggaatc ggggcttgct ccggaagaag
     4621 tggatgcggt ggcggggccg ccgatggggc gtcccaagag tgcggcattc cgtacggcgg
     4681 atctggtggg gctcgatacc ttcgtgcatg tggcggacaa ctgtcatcgg ctcttggccg
     4741 acgatccgca gcgtgatgtg tttttggtcc ctcctttcat tcggacgatg gtcgagcgtg
     4801 gccagattgg aaacaaggcc aagggtgggt tttataaaaa agacaaggcc aagaatgtgc
     4861 tgacgctcga tccggcaacg ggcgagtacc gggacaaact tgcgaaaagc gatgtccttg
     4921 gtttttgcaa gtctctcaaa gatatcgatg aggtgggggc tcgtgttcgc aagctgatgt
     4981 cggataccgg accggcggga gactatgctt ggaaggtagc ggccaagacg ctgttatacg
     5041 cggcggggct ggtgggggag atttgcgacg atgtgacggc gatcgatgat gcgatgcgct
     5101 ggggatacaa ctgggagctt gggcctttcg agatgtggga tgcccttggg tttgcgcgga
     5161 cggtcgaccg aatgcaaaaa gatggtttcg aaatccccgc atcgatacgc aagatgaagg
     5221 acgatggggt gaaggggttt tatgacggcg acaagattta cgatcttgtg gcgggcaagc
     5281 aaatcgcgcg caaaaccgat cctcgcacct tacctttttc gatcgttcgg ggcgaaaagg
     5341 cgatcgaaaa actcgagggg gccagcctgt gggatacggg cgatggggtt ctcgcggtca
     5401 cgttccacac caaggccaat agcatcgatc atgatgtcat tgcgatgatc gaaaagggcg
     5461 tgacgcgagc ggagaacgag gcgcgcgcgc tcgtactgtt caacgagggg gagcattttt
     5521 gcgtaggcgc gaacttgatg ctgatcgtca ccgcggcgat aaacaaaaaa tatgaagata
     5581 ttcgccaagt tgcagccgct ttgcaggctg ctgggcagcg gatgaaatat gccaaggtgc
     5641 cggtggtgac ggctccattc agtgtcaccg tggggggtgg tttggagcta tgcatggccg
     5701 cggggcatgt ccaggcagcg gctgaaacct actgcggact ggtggaagtg ggcgtgggtt
     5761 tgattcccgc cggtggcggt tgtacgaacg cgatgtggcg ggcactggaa aacgttccag
     5821 aaggagcgcc agcggatatc tacaacttga ccacccaggt gttcaaaaat atcgcgcttg
     5881 ccaaagtatg caccagtgcc ctcgaaggcc agcgcgtcgg ctacttccgc tcgggcgatg
     5941 gcatcacgtt cgacaaagcc cgtctgcttc acgatgccag aaagtatgcg atcggtcttg
     6001 ccgagcgtgg ataccatccg ccgattccta gaagtcataa actctttggc gaaagcggca
     6061 tcgctacctt ccgcatgatg gtctcggcgc tcgttgcggg cggacaggct accgcgcacg
     6121 atggtgtggt ggcgacgcgc ctggccgagg tgttgtgtgg tggagtcgat ggcgccgctg
     6181 ctcccgtgac cgaagagcga atgctcgaac tcgaggtgga ggcatttctc agcctgtgtg
     6241 gtgaggaaaa gagcatcgcc cgcatgcagg ccatgctcac caccaacaaa ccgttgcgca
     6301 actaattgaa ctgggtcttg cccgtgtgca tgaccccatc gaggctgatg aatcgaatga
     6361 atggacgcac ccacaagaag gccaaaaccc gattggaaat gcgtctttcc tcaacggaat
     6421 gagggtttct catgcgtgaa gttgtcatcg ctgcgtcgta tcgttccgca tgcggacgcg
     6481 cccacaaggg cacgctcgcg ttgacccgtc ccgacgatct gcttgcccag gtgattgctg
     6541 gagtgctcga aagagtgcct cagatcaagc cggagatgat cgaagatgtg gtgattggtt
     6601 gtgccatgcc ggaggccgag cagggcatga atgtggcgcg atgttccgcc atgcttgcag
     6661 gccttcctca gcacgtgccg gccatcacca ccaaccgttt ctgctcgagc ggattgcagg
     6721 ccatcgccac cgctgcccat caaatcgctt tcggtgaaat ggatatcgcc ctggcgggtg
     6781 gggtcgaatc catgaccatg gtccccatgg gcggcaacaa actcaccgcg agcgccgcca
     6841 tcatggaccg cgtcgccgct gtctacactc ccatgggcat caccgccgaa aacgtcgcca
     6901 agcgcttcga ggtctcgagg gccgtgcaag acgaattcgc ctaccacagc cagatcaaag
     6961 ccgcgaccgc ctcacaaaac ggacttttcc gggacgaaat catccccgtg aaagcaacgc
     7021 gttatgccac caaaaacggc gttcgcgtac gcgacgacgt ggtcttcgac accgaggaat
     7081 tgccgcgtat ggatacgacc ctcgaagggt tggccgcgct acgtcccgcg ttttccgcca
     7141 ccggctccgt aacggcgggc aatagttcgc cactgaccga tggcgcggct atctccttgc
     7201 tgacgaccaa agagcgtgct caacaactgg gtcttgaaat tctcgcctat tttcgcgggt
     7261 ttcaagtggt gggggtcgat cccgagatca tgggtgtcgg ccccgcttac gccgtcccca
     7321 agttgctcac ccgctacaac ttgaccctgg acgatatcga tcttgtggaa atgaacgagg
     7381 catttgcctc ccaatccgta tactgcctgc gccaactcgg gctgcccgcc gaaaaagtca
     7441 atgtcaacgg gggtgccatc gccctgggac atcccctcgg tgccacggga gccaagctta
     7501 cggctaccct tctgcacgaa atgaagcgtc gcaacgccaa acgtggtatc gtgaccatgt
     7561 gtatcggcgg cggtatgggt gcggcaggac tatttgaacg tccgtaactc cgattattcc
     7621 tttcacgtct ggctctgtct attttagcct cacccatgaa ggttgctttg cccgcaagca
     7681 ccgtttggga gtagcctacc cgcgctgccc catctgttcg atgttcaggt gatcccagag
     7741 gattgtcgcc gtgccgccgt cgttgccttc tccgcatccg tttcgaccga gaaaaatcgg
     7801 tcgttacttg ctgcatcgtg agatcgcttt cggaggaatg gccaccgttc atatcggtcg
     7861 tttgctcggc caagtcggct tctctcgaac cgtggccatc aagcgtatgc acgatcattg
     7921 cgcgcgggat cctgagttcg tgtcgatgtt catcgacgaa gctcgactgg cagcccgtat
     7981 ccgccatccc cacgtcgttc ctaccctcga cgtcgtggcg ctcgaaggcg agcttttcct
     8041 ggtgatggaa ttcgttcagg gagagacgct cgctcgcttg attcgtcgtt cggtcaaagc
     8101 tcggatccca atctccctac aagtggctgc cgccgtgatc tccggggtcc tcgagggact
     8161 ccatgcggcg catgaggcga ttggtgagca tggcgagttt ttgggcattg ttcatcgcga
     8221 cgtttctacc caaaatataa tggtagggac ggatggcgtg tccagagtcc tggacttcgg
     8281 tgtagccaag gccatcaatc gcatccagac gacccgtgat ggtcagatca aaggcaagat
     8341 tgagtatatg gcgcccgagc aaatccgcgg tgaagccgtg gatcgtcgga cggacatcta
     8401 ttcggtgggg tgttgtctct gggaaatgtt ggtgggccgc agactgtatg tggccgataa
     8461 tcaagtcacc ttatggggaa tggttctcca gggaaacccg ccaaagccat cttctgtcaa
     8521 tcccaacgtc actcccgaac tcgatgccat caccatgcga ggtctcgagg cggatccgtc
     8581 gaaacgctat gaaaccgccg aacaaatggc gatcgaactc gagcaaaacg tggggattgc
     8641 cacaccacgg gaggtgggcc gatgggtcca gtctctcgtg agcaagaaga tgcaagccct
     8701 cgatgaagtg ttggctgaac tcgacagtgt ctcgaagagt atttccgttt ttgcaagcga
     8761 ccaaaccagc gccgagcata cggcgctttc cgcggaacaa ccggccgtgc ccgtgccgtc
     8821 gcccgtgcag cgtcactctt ctccgaatca tccggtcgtg gtgcctaccc caatccctga
     8881 tgccgatcag ccttcacaac cacgtcgccc ccaagcgtcc gctcccgtca tcaacgttcc
     8941 acgtccgcct atcccgtccg tgcctgaacc gcggccgccc cgtcgttcat cccaacctcg
     9001 gatatcccgg ctttcccctg tccccccacc gcgccagcat caaaataaag ataatatttc
     9061 caataagtta caacctgggg gatcatcgac ggacacaccg cctcccaccg cgatggcgcc
     9121 ctccaattcc gaatcgtcga aagatgcggc ggcgttgttg cccaggactc cccgaccttc
     9181 cgacagttat ttgcgagccg aaaacgaaag ccctgcggat agcgccatcg ctgcacagac
     9241 agcaaaaagc cctgatcgta cgtccgcaat ttcgtttcct ctaccatccg ctggcggtgg
     9301 cagtccgatg ctccccacgg atttctccga ggtggccgcg gaggcatcgg agcaacatac
     9361 tccgccaact tctgaacaag gggcttccac cgatccagct tctgccgaag gagcatcctc
     9421 tccaccgtct ggggccgaac cagccgatac cgtgcaagcg atagcttcgg attccccggg
     9481 cgttgctccg ttgttggacc cttcccctgt cgtatcgtcg gatttgtttt cgggatcaac
     9541 gcaagatcca ttcggtttta cgcgcaagaa aagcaaggcg aagtggtggg gggcgggggg
     9601 aatcggtctt ggattggccg tgatcgcggt gcttgtgggg acatggaagg gcgcgcaaac
     9661 cacggagccg tccgctcctt ctcccgacgg gtccgtatct tcctcgatgc cgcgggttcc
     9721 ggacagcgtg caagtcgcgg agcaggcttc gccggtggcc tccgcgagcg gttcggaaac
     9781 gcaggatgcg gcgccagcca cggtgcgttt gcggctcgaa ctcagcccgc gtacggccaa
     9841 agtcgctgtg gatggggttt ccgtttcgga gtcggaaatt cgtcttcccc tatccgacaa
     9901 gcccgtgaag attgtcgcgg aggccgcggg attttccccg atggaaaggg cgtttgtccc
     9961 ttccaaggac ggagtcgtgg tgtttgaact caaacggctg ccttcgaccg tgagaccaac
    10021 cacg
//
LOCUS       DAOSOR010000160         9998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6740_length_9998_cov_4.805491, whole genome shotgun sequence.
ACCESSION   DAOSOR010000160 DAOSOR010000000
VERSION     DAOSOR010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9998
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6740_length_9998_cov_4.805491"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PK710_10495"
     CDS             <1..290
                     /locus_tag="PK710_10495"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1848 family protein"
                     /protein_id="HOT10191.1"
                     /translation="ASAMRMAPTRKGCGCAFARDIGAYHTCGHGCVYCYANESPEAGL
                     RHARQIKARSPHLGTGILSINSNGSINTGASSQLDLGLLSSQVSVDTEAMR"
     gene            303..1787
                     /locus_tag="PK710_10500"
     CDS             303..1787
                     /locus_tag="PK710_10500"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT10192.1"
                     /translation="MTDTGSTHDTAMWIGREVDAKYRILRLIGQGGMGAVYEAENLAL
                     GKRVALKIIDVESATQPDAVERFEREARMAAAIESANVVQVFDVGIAENRPYLVMELL
                     RGETLGARLQREGTLSVSDTVRMAAQVLRGLHRAHEKGVVHRDLKPENIFLARTDDES
                     WVAKIVDFGICKNTDRTGALGCHTLTQKGVILGTPHYMAPEQAQGLADLDGRADLWSV
                     GVIMFECLAGHRPFSGDNYEQVIIAICTQSPPSLRGVAGVADPIADVVSRAMRQSRDK
                     RFSSAREYLEALRLAVPESMQGELLGADTIRDREVNAKENDVLSEQNRLPRSGGGLNR
                     KRRLRSYLVGLAVTVGFVVSIAAWAVWNRGPVVGTGSVGSYAPAISADASAVIRVETN
                     VQGASVFVGDRQMVDGLVDGLRGETVEIRVQAPGYETQWRTLRFEGKQEVIRIELREQ
                     PKPQSASSSVSPTPSGPRNVTTSRPAGVGSGNLTGGLELKTELP"
     gene            2620..3933
                     /locus_tag="PK710_10505"
     CDS             2620..3933
                     /locus_tag="PK710_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10193.1"
                     /translation="MGKLLKTIFVGVLGAASFGLLACGSEPGSPSPSGPEVAGPVEAP
                     PLAPGVAKIGTWRGEWNATTQKLTFTEIEADNSAGFDVGMQPQGFDKVKSGKIRFSTE
                     YSYVGEEDDEDCPLGRVCGRVRVENISTRLLNSVYVEIVSLSPAEYTAHDFSDEVPPG
                     YPMSNQYGLWGYNNLGPGNAASRDWTFANPAGQSFSFSVRVLAIFTRTNYDLSFNDVS
                     EPGNTGTADWDNNSPVWRDACTHGTTLVSSSTSSFAASITMPFPFTITNKTFSTNVGE
                     LWVTSYGLGWSKAPEPILPASLPASGYINTLFPFWEQVEGAQVCHSTEGLLPNRRFIV
                     TWKNAHLKGLPNSNLTFSAVLQEKTDNVFFLYHRWSDGSDDCASGTSNRVRNESAAIG
                     IQGDNNYFPSNPVGLFSPHAANCPGSGYFVKFEAQPANKFFPPSP"
     gene            3976..4152
                     /locus_tag="PK710_10510"
     CDS             3976..4152
                     /locus_tag="PK710_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10194.1"
                     /translation="MDTKHNHPRDSENVQSNEARQPYEAPRVEKRRSLQQVTLTSGMG
                     QSAVSQAGFISETN"
     gene            4185..5201
                     /locus_tag="PK710_10515"
     CDS             4185..5201
                     /locus_tag="PK710_10515"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT10195.1"
                     /translation="MTDRCQLTCKHCLRDPGKRPTDLPLPLVRSSLEQAKCFLRSFHA
                     AFTGGEPVLHPEFSAILDAAVDLDYTWHMVTNGQRFERVIDTLLERPLRKEKLTALTI
                     SLDGADEATHDAIRSPGSYREVMKAASLCMAHEIPFAFNTTLHAKNVHQLESMGLLAS
                     QLGADRISFILFSATGTKHDAELYLSARQWRNVMDRIDRLAEALTIRVNIPEGFHRKQ
                     PWHVCAPFTGQQIHVDVHGRVILCCQHAGIPRDPNDNRDIVGSLYEMNLAEACRRFLG
                     IVHRFQEDKMLAMERGELKDDWDYFPCNYCMKYFGKPYWTSEGAAGPSAIRARRKKRL
                     PVLG"
     gene            5215..6192
                     /locus_tag="PK710_10520"
     CDS             5215..6192
                     /locus_tag="PK710_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10196.1"
                     /translation="MNLPRFVSFHDVVIERPGESLDADAFDALFRPFEPQNCGNSRAD
                     ITIVVEHRKEPFPVPSDLPPIFYFGHVQAYGADDVSVISDRYSRLDLRFDKKTLTMGL
                     CPQSEKSLPGVTQAMQHLGLGLMLREYGIFGLHAAGICFGEHALLLIGDCRVGKTTTT
                     LALSVHATGFLGDDRVLFRNDGKTTTLFSYPREFHVDDETAKAFALSVDPSARSMEGK
                     YALAPEHVFRVPHQPRHRGPITLLFPTILGDDTSLVVPMTKARAYGELLVASALTSVQ
                     GVRHRQENLDGLERLVNEGRTFEVHLGRDLLQRPEQASKELLRAVGVYP"
     gene            6189..7757
                     /locus_tag="PK710_10525"
     CDS             6189..7757
                     /locus_tag="PK710_10525"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT10197.1"
                     /translation="MKLLWSPRAATRLWRLWAASWPTIVLLVLLRSVWLLAIPIAVLS
                     VGTRVPVVLPMVAGTLFDALSLMLQQRLRRSLRTSCMVTSARDALGKQGGVPESQIHA
                     AFWAAYVSEYAISSDLPSMLAALMSTALILVFASFRMGLDLVLPVTGVLAIVGGLGFF
                     LHRRLRPRLHITVKNRARTADWLSAAERDAGEIGGTRSEEPFVAKVREATHNWCSAED
                     TVERKQIGHRFVLLLLLGIGLAFVLSYRGIDWMHLAFGNEGEQFSLRTVADVLLLVTA
                     IPVALKVARHANALLTAYGELAEMHPPDRVPRLSTQAWKEPPRGLVVRDLLVKYGDKL
                     ALHIPFWQASFEKPVGILGPNGSGKTTFASVLGGVLAPTEGNVLVGGVPSQQIDRNDV
                     AFVPQDPVLIEALTIEENIRLVVPLATTQQQEAILQRLGLHKPLDQKTGELSRGERRR
                     IALARALLKESQLLILDETDAWLDDAGRRILFEILQEETKKRAVVIVSHRQEVLTVVG
                     DVLRLDESHQMVVD"
     gene            complement(7771..8526)
                     /locus_tag="PK710_10530"
     CDS             complement(7771..8526)
                     /locus_tag="PK710_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10198.1"
                     /translation="MSVPRVMLEPGGQQETMRAFRFMVGTAAVAIFALGCGSTEDTHS
                     NSGKGDAMTDGQVQVDGATDAGKDGPVEVSPDVQGDVIPETQPDTPMEAEPDTPGEAQ
                     PDSPEEAQPDSPAEVEPDVLIDSPVDVAQDTAPDTANDVNFDADTGDAAEFQLVEVDL
                     DLRSNCIIEFSPSFVEVVAGNPIKVRVSNVSTSYATSLWSSSGSGVFGLPPGQSWDSE
                     EFCSGQYPMESHIQAFNHNDSSCNPVKLPIHCK"
     gene            8616..9050
                     /locus_tag="PK710_10535"
     CDS             8616..9050
                     /locus_tag="PK710_10535"
                     /inference="COORDINATES: protein motif:HMM:NF024862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HOT10199.1"
                     /translation="MVARFARRYGQARLAARLFRIRCGLSRRLALEPLDVLVRDLGRV
                     APRFGVSFEDALSVIRVVEPVVARCPWIENTCLFRALGRFAVLRSEGFSVEVLMGMGE
                     DGTSDTGHAWILVDGVAFDADDAHEASRMAVTFRSSSLESPG"
     gene            9047..9322
                     /locus_tag="PK710_10540"
     CDS             9047..9322
                     /locus_tag="PK710_10540"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOT10200.1"
                     /translation="MNSPRYSPNSRCLFTELRDGTAVLLHLDTKFYYTLNPTGVVVWK
                     GLSEAVTLDEIVNRIVSAFETDETTARSDIQKLLTELVDEELVEATS"
     gene            complement(9312..>9998)
                     /locus_tag="PK710_10545"
     CDS             complement(9312..>9998)
                     /locus_tag="PK710_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10201.1"
                     /translation="LRGGSTHENGLFPWERAVITSSSFPHEGRLLLGAVGGGRELPGL
                     LDLGFHVTAFEPNDVLVAGAQHLASTLHGAQVHQGSYADLIRAIHDGDGPLAEALSGP
                     PFDGIILGWGSLTHVIDEQERRELLTALRILAPTAPVLISFFLRSDKPNQGNSERLRH
                     WLRPWLDRAGAPYRPEPGVLVDPRFGFVYAFTEKEIYDLAFSCGYRVELLKPCDFPHA
                     LLVPIDPPTT"
BASE COUNT         2110 a         2580 c         2954 g         2354 t
ORIGIN      
        1 ctgcttcggc gatgcgaatg gctccgaccc gcaagggctg tggttgcgct tttgcccgcg
       61 atattggcgc ttatcatacc tgcggccatg gttgcgtgta ttgttatgcg aacgagtctc
      121 ccgaggcggg gcttcgccac gcgcgccaga tcaaagctcg ctctccgcac ctcggaaccg
      181 gaatattatc aataaattca aatggttcta tcaataccgg agcaagctcg cagctcgatc
      241 tgggcctctt gtcatcgcaa gtttcggtgg ataccgaagc gatgcgctaa cctggaggcc
      301 tcatgacaga cacgggttcc acccacgaca ccgcgatgtg gatcggccgt gaggtcgatg
      361 cgaagtaccg tatcctgcgg ctcattgggc aaggcgggat gggggcggta tacgaggcgg
      421 agaatcttgc tttgggaaaa cgtgtggccc tgaagatcat cgatgtcgag tcggccacgc
      481 aacccgatgc ggtcgagcgt ttcgagcgag aagcgcgcat ggctgctgct atcgaaagcg
      541 ctaacgtggt ccaggttttc gatgtgggga tcgccgaaaa cagaccctat ctggtcatgg
      601 aattgcttcg cggcgagact cttggcgcaa ggcttcaacg ggaaggcacg ctatcggttt
      661 ccgacacggt acggatggcc gcgcaagtgc ttcgcggcct tcatcgcgcg catgaaaaag
      721 gggtcgtgca tcgggacttg aagcccgaaa acatcttcct tgcccgcacc gacgatgagt
      781 cgtgggttgc caagatcgtg gacttcggca tctgcaaaaa caccgacaga accggggcct
      841 tggggtgcca taccctcacg caaaagggag tcattcttgg aacgccccat tatatggcgc
      901 ccgagcaggc gcaggggttg gcggatctgg atggtcgcgc ggacttatgg tccgtgggtg
      961 tgatcatgtt cgaatgtctg gccggtcatc gtcctttttc cggggacaat tacgaacagg
     1021 tgatcatcgc catttgtacg caatcgccgc cgtcgttgcg gggggtggcg ggcgtggccg
     1081 atcccatcgc ggatgtggtt tctcgagcga tgcgccaatc gcgggacaag cgtttttctt
     1141 cggcgcggga gtatctcgaa gcgttgcggc ttgcggtgcc cgagtcgatg caaggcgagc
     1201 tgctcggcgc cgatacgatt cgtgatcggg aggtcaacgc aaaagagaac gatgtgctgt
     1261 cggagcagaa ccggttgccg cggtcaggcg gtggactgaa tcgaaagcgg cgactgcgaa
     1321 gctatttggt ggggctggcg gtaaccgtag ggttcgtcgt ttcgatagcg gcctgggcag
     1381 tatggaaccg cggtcctgtt gtgggaacgg ggtcagtggg tagctacgcg cccgcgattt
     1441 ccgccgacgc ttcggcggtg atccgagtgg agaccaatgt gcagggggcg agtgttttcg
     1501 tgggagatcg gcagatggtc gatgggctgg tcgacggcct gcggggcgag acggtcgaga
     1561 ttcgtgtgca agcgccgggg tatgagacgc agtggcgcac gttgcgattc gaagggaagc
     1621 aagaggtgat acggattgaa ttgcgagaac agccgaagcc gcagagtgcg tcgtcgtcgg
     1681 tgtcccctac cccttcgggg ccgcgaaatg ttacgacttc tcgcccggcg ggggttggat
     1741 cgggcaatct caccggtggt ttggagttga agacggaact gccttgaggg gggggagagt
     1801 ggattgttgg ggagattacc ccatttcccc gggtctttcc ttcacggtca cgctgaccgt
     1861 cacgttcacg gttgcggccc acgcaaaccg gtcacggtca acctctgagc cattcgcgcg
     1921 tgcgggcggg tttgaaaccc gcccctacgg tgccggttgt tgccctgagc catcccctca
     1981 tttttccccg cgccaaaaat tctcagttcg tatcccttat tcaattcggc aaaaatgagg
     2041 catctatcag ctattggctg gattgcttgg ttcgcctggc tagcgggtag cgcggccgtg
     2101 agccgatcag catattctcc gtaactattg aacaaatatt ccttttttgc tctcccggac
     2161 atttcctggt ccgctatagt tcaatcaaat ggggtaatag aatacaaaaa tcgtgagtgg
     2221 aacaagcttg tggttgagtc tgtggtgaat tacaaatagc ttgttccccg atgacttgct
     2281 tgttgtgatg ggcaggggaa acgaggtgtc ggatattgtg gttttccacg cacggaatat
     2341 gctgacagga aagcatgggc gaaatacatg gaaaatagat ggatatcgac agggaatcag
     2401 acggggcgag aggttgcggc ttgacgaatt tgcgctagag tttctggatc acgattgaaa
     2461 atagtgggtc atgggggatg atgatgaatc tttcctcatg aggccgcatc gaaaaaatgg
     2521 tcgtgtcctg gttacgggca cgttgacggg aatcattcga cggaatacac tgagcaccgg
     2581 tttgcaagcg ggtgaatcgc gagggttgga ggcttgacca tgggaaaatt gttgaaaacg
     2641 atattcgttg gagttttggg cgcggcaagt tttgggctgc tggcctgtgg ttcggagcct
     2701 ggttcgccct cgccgtcggg gccggaggtt gcggggccgg tggaggctcc gccccttgcg
     2761 ccaggggtgg ctaagatcgg aacttggcga ggggagtgga atgctaccac ccaaaagctt
     2821 acgttcaccg aaattgaagc agataattcg gccggattcg atgtggggat gcagcctcag
     2881 ggattcgata aagtaaaatc ggggaaaatc cgttttagta cggaatattc atatgttggc
     2941 gaagaagatg atgaagattg cccccttgga cgcgtctgtg gtcgagtcag ggtagaaaat
     3001 atcagtaccc gtctgctcaa tagtgtgtat gtagaaatcg tctccttgtc acctgcagaa
     3061 tacactgcac acgacttctc cgacgaggtt ccaccagggt acccaatgtc caaccagtat
     3121 ggattatggg gctacaataa cctaggccct ggaaatgctg cttccagaga ctggacattt
     3181 gccaatccgg cgggacaaag ctttagtttt tccgttcggg tattggctat ctttacgcga
     3241 accaattacg acctttcttt caatgacgta agcgaaccag gaaacactgg gacggcagac
     3301 tgggataaca attcacctgt gtggcgagac gcttgtacgc atggaacaac acttgtttcg
     3361 agttccacct ctagttttgc cgcttccatt accatgccgt tcccttttac catcacgaac
     3421 aaaacatttt caaccaatgt cggagaactg tgggtaacct cctatggttt ggggtggagc
     3481 aaagcccctg agccaattct ccctgcgagt cttcctgcct cgggttatat taataccctt
     3541 tttcctttct gggaacaggt ggaaggggcg caagtctgtc attctacaga ggggttgttg
     3601 cccaatcgtc gattcatcgt cacatggaaa aatgctcacc taaagggcct tccgaacagc
     3661 aatttgacat ttagtgctgt attacaagaa aaaacggata acgttttctt cctttaccat
     3721 cgatggagcg atggttctga tgactgcgct tcaggaacta gcaaccgtgt acgcaatgaa
     3781 agtgcagcga ttggcattca gggagataac aactattttc catcaaatcc ggttggtcta
     3841 ttttcgcccc atgcggctaa ttgcccagga tcgggttact tcgtgaaatt cgaagcccaa
     3901 cctgccaaca agttttttcc tccaagccca taaaaccaca ccacgtcatt tcaatcgtct
     3961 ccaatgagtg aacgcatgga taccaagcat aatcaccccc gggactctga aaacgttcag
     4021 agcaatgaag cgcgccagcc ctacgaggct cctcgggtag aaaaacgacg ctccctccaa
     4081 caggtcaccc tcaccagtgg tatgggccag tcggccgtca gtcaggctgg tttcatcagc
     4141 gaaacgaatt aatccttttg gcaggcgatt ggattacgtt cgagttgaca gatcgctgtc
     4201 aactcacctg caagcattgc cttcgtgatc caggcaaaag gcccacggac cttcccctgc
     4261 ctctcgtccg cagttcgctc gaacaagcaa agtgttttct tcgctccttc cacgctgcct
     4321 ttacgggagg agagccggtt ttgcacccag agttttccgc cattctcgat gctgccgtcg
     4381 acctcgacta cacctggcat atggtgacca atggtcaacg gttcgagcgc gtcatcgaca
     4441 cccttctcga aaggccccta cgaaaagaaa agctcaccgc gctgaccatc agcctagatg
     4501 gcgcggatga agccacccac gacgccatcc gctctccggg aagttaccgc gaagtcatga
     4561 aagcagcctc cttgtgcatg gcccatgaga tcccctttgc tttcaacact actcttcacg
     4621 caaaaaacgt ccaccaactc gaaagcatgg ggctgctcgc ctcccaactc ggagccgacc
     4681 gaatttcctt catcctgttt tccgccacag gcacaaaaca cgatgccgag ctttatctat
     4741 cggcgcgtca atggcgaaac gtaatggatc gcatcgaccg gctggccgaa gctcttacca
     4801 tccgcgtgaa tatccccgaa ggattccatc gaaaacagcc gtggcacgtt tgcgctccct
     4861 tcacaggaca gcaaattcat gtcgacgtgc atggacgtgt cattttgtgc tgccaacatg
     4921 cgggtatccc ccgggacccc aacgacaatc gagatattgt aggcagtctt tatgaaatga
     4981 accttgccga agcatgccgt cgcttcttgg gcatcgtgca tcgattccaa gaagacaaga
     5041 tgttggcgat ggaacgcgga gaattgaaag acgattggga ctactttccc tgcaactact
     5101 gcatgaagta ttttggcaaa ccgtattgga cctcagaagg cgcagcggga ccttcggcga
     5161 tccgcgcccg acgcaaaaag aggcttcctg tgctaggctg acgccgcttt ggacgtgaac
     5221 ctacctcgct tcgtttcctt ccatgacgtg gtgatcgaac ggcccgggga gtcgttggat
     5281 gcagatgcgt tcgatgcgct atttcgacct tttgaaccgc agaattgcgg caattcccga
     5341 gcggatatta cgatcgtggt cgagcaccga aaagaacctt tcccagttcc ttccgactta
     5401 ccgcccatct tttacttcgg tcatgtgcag gcctacgggg cagatgatgt atccgttatc
     5461 tcggaccgat actctcggct cgatctgcga ttcgacaaaa agacgctgac aatgggtcta
     5521 tgcccccaaa gtgagaagtc gcttccgggt gtgacccaag cgatgcagca ccttggattg
     5581 gggctgatgc tccgcgagta tggtatcttt ggtttgcatg ctgcgggcat ttgctttggt
     5641 gaacacgctc ttcttttgat cggagactgc cgggtgggaa aaaccaccac aacccttgcc
     5701 ctttccgttc atgcaacggg atttcttggg gatgatcgcg tcctttttcg caacgatggt
     5761 aagacaacga cgctcttttc ctatcctcgg gaatttcacg tggatgatga gaccgcaaag
     5821 gctttcgcat tgtccgtcga cccaagcgcc cggagcatgg agggcaagta tgctcttgct
     5881 ccagaacacg tttttcgggt ccctcaccaa ccccgtcatc gaggtcctat cactctgctt
     5941 tttcccacga ttttggggga cgatacgtcg cttgtcgtcc ctatgacgaa ggctcgtgcc
     6001 tatggggaat tgctagtggc aagtgctttg acctccgtgc agggggttcg tcatcgacaa
     6061 gaaaatctcg acggtttgga gcgattggtc aacgaagggc gaaccttcga agtgcatctc
     6121 gggcgagatt tgttgcagcg cccggaacaa gcgtcgaagg agcttttacg cgcggtgggc
     6181 gtgtacccat gaagctgctc tggtctcccc gagcagccac acggctgtgg aggctctggg
     6241 cagcgtcatg gccgaccatc gttcttctcg ttctgctgcg ctccgtctgg ctgctcgcta
     6301 tcccgatcgc ggtcctttcc gtgggaacac gagttcccgt cgtgcttccg atggtggctg
     6361 gcacactgtt cgatgccctt tccttaatgc ttcagcagcg gctgcgtcgc agccttcgga
     6421 cttcttgcat ggtgaccagt gctcgcgatg ccctgggcaa acaggggggg gtaccggagt
     6481 cgcaaatcca tgcggctttc tgggcggcgt atgtgtcgga atacgctatt tcctccgatc
     6541 ttccgtcgat gcttgcagcg ctaatgtcca ccgcgctgat tctcgtattt gcgagttttc
     6601 gtatgggact ggatttggtt cttccggtca ccggcgtgct tgccatcgtg ggtgggcttg
     6661 ggttctttct tcatcgtcgt cttcgccctc gcctgcacat caccgtgaaa aaccgtgccc
     6721 ggacagcgga ttggctttct gccgcagaac gggatgcggg agaaattggt ggaacgcgct
     6781 cggaagaacc tttcgttgcg aaggtgcgcg aggccacgca caattggtgc tccgcggaag
     6841 acacggtgga gcgcaagcaa atcggacatc gttttgttct gcttttgctt cttggaatcg
     6901 ggctggcgtt tgtattgtct tatcgaggca tcgattggat gcacttggct ttcggaaacg
     6961 aaggcgaaca attctcgttg cgtacggtgg cagatgtcct tcttttggtc acagcgattc
     7021 cggttgcttt gaaggtggcc cggcatgcca atgcactatt gaccgcgtat ggggagcttg
     7081 cggaaatgca tcccccggat agagtccctc gtctttcaac acaggcttgg aaggagccac
     7141 cgcgagggtt ggtggtacga gatcttttgg tgaaatacgg agacaagctg gctcttcaca
     7201 ttcctttttg gcaggcatcc tttgaaaaac ccgttgggat cctgggaccg aacggctcag
     7261 ggaaaaccac cttcgcctcg gtcttgggtg gtgttttggc tcccaccgaa gggaatgtgc
     7321 tagtgggagg cgttcccagt cagcagatcg accgcaatga tgtggctttc gtaccgcaag
     7381 atcccgtact aatcgaagcg ctcaccatcg aagaaaacat tcgtctcgtg gttccccttg
     7441 ccaccacaca acaacaagaa gcgattcttc aacgtctcgg attgcacaag cccctggatc
     7501 aaaaaacggg agagctatcg aggggggaac gtcgaagaat tgcgttggca agagcgctgt
     7561 tgaaggagtc gcagcttctg attctcgatg aaacggatgc gtggctcgat gatgcaggaa
     7621 gacgtattct tttcgaaatc ctgcaagaag agacgaaaaa gcgggctgtg gtcattgttt
     7681 cccatcgtca ggaggtcctt accgtggtgg gagatgtgct tcgactcgat gaaagccatc
     7741 aaatggtcgt ggattgatcg atgggatggt ttacttgcag tggatgggaa gtttgacagg
     7801 attacatgac gagtcgttgt gattgaaggc ttggatatgg gattccatcg gatactggcc
     7861 ggaacagaat tcttctgagt cccatgattg ccccggagga agaccaaaca caccggaacc
     7921 gctggaactc caaagactcg tagcatagct ggtgctgaca ttgctcacac gcaccttgat
     7981 gggattgccc gccacgactt cgacaaaaga cggcgagaat tcgatgatgc aattggacct
     8041 gagatccaag tccacttcca caagctgaaa ctccgctgcg tctccggtat ccgcatcaaa
     8101 gttgacatca tttgccgtgt caggcgcggt atcctgggca acatcgacag gagaatcgat
     8161 aaggacatcg ggttcgactt cggcaggact atcgggttgt gcttcttcgg gactatccgg
     8221 ttgtgcttcc ccaggcgtgt caggctcggc ttccatgggt gtatcaggct gggtttccgg
     8281 tataacatcg ccttgaacat cgggggaaac ctcgacgggc ccgtccttgc ccgcgtccgt
     8341 ggcgccatcc acctgcactt ggccatcggt catggcatca cccttgccgg agttcgagtg
     8401 ggtatcttct gtcgagccgc atcccaaagc gaaaatcgcc acagcggctg ttccaaccat
     8461 gaatctgaac gcgcgcatcg tctcttgttg ccctccaggt tcgagcataa cacgcggaac
     8521 ggacatgtgg cactcgttcc ctcacggccg agcggtcttg gggcgttgat ttccaggcac
     8581 ttgtcagtcc tgatttccga gcccacactg ccagggtggt tgcaaggttt gctcgtcgct
     8641 acggccaggc gcgactcgct gcccgtctct ttcggattcg gtgcggcctg tcacgacgtc
     8701 ttgctttgga acctctcgat gttctggtcc gggaccttgg aagggtagcg cctcgtttcg
     8761 gcgtgagctt cgaggatgcc ctttccgtca tccgtgtggt ggaaccggtg gtggccaggt
     8821 gtccatggat tgaaaacacg tgtttattca gggccttagg tcggtttgcg gtattgcgga
     8881 gcgaagggtt ttcggtggaa gttttgatgg gtatgggaga ggacgggacg agcgacacgg
     8941 ggcatgcatg gattcttgtc gatggggttg cattcgatgc ggatgatgcg catgaagcct
     9001 cgaggatggc ggtcactttc cgctcctctt cgttggagtc accaggatga attcaccgcg
     9061 ttattcccca aattctcgct gtttattcac ggagttacgt gatggaacgg cggttttgct
     9121 gcacctggat acgaagtttt attacacgct caatcccacg ggtgtcgtgg tgtggaaggg
     9181 attgagcgag gcggtcactc tcgacgagat cgtgaatcgg atcgtgagcg cgttcgagac
     9241 ggatgagaca acggcgcgtt ctgatattca gaaactattg acggagcttg tggacgagga
     9301 gctggtggag gctacgtcgt aggtgggtcg ataggcacga gaagagcgtg ggggaagtcg
     9361 caaggtttga gtagttcgac gcgatagccg caggaaaaag cgaggtcgta tatttccttt
     9421 tcggtgaagg cgtagacgaa accgaagcgc gggtcgacga gcacgcccgg ttcgggtcga
     9481 tagggggcgc cagcacgatc cagccatggt cgaagccaat ggcgcaagcg ttcggagttg
     9541 ccttggttcg gcttatcgga gcgcaagaaa aagctgatga gcacgggagc ggtgggagca
     9601 aggatacgta gggccgtcag aagctcgcgt cgctcttgtt catcgatgac gtgtgtcaag
     9661 cttccccaac ctaggatgat gccgtcgaag gggggaccgc tcagggcttc cgcaaggggg
     9721 ccgtcgccgt catggatagc tcgaatgaga tccgcgtagc ttccttgatg cacttgggcg
     9781 ccgtgcaggg tggatgcgag gtgttgtgct ccagcgacga gcacgtcgtt gggttcgaaa
     9841 gcggtgacgt gaaagccgag gtcgagcagt cccggtagtt cccttcctcc gccaacagct
     9901 ccaaggagca atcgaccttc gtggggaaaa gaagaggagg taatgaccgc gcgttcccag
     9961 gggaacaggc cgttttcgtg ggtgctgccg cctcgaag
//
LOCUS       DAOSOR010000161         9923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6794_length_9923_cov_3.337860, whole genome shotgun sequence.
ACCESSION   DAOSOR010000161 DAOSOR010000000
VERSION     DAOSOR010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9923
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6794_length_9923_cov_3.337860"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="PK710_10550"
     CDS             <1..1041
                     /locus_tag="PK710_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HOT10202.1"
                     /translation="GTTNLVATGLTGPACGVVVNALATSSDANVLATPHIIATDNVPA
                     EISVGQNIPLQTNLGGLGSMGSLAGLAGGQAGSAASSLGALSSFGFGGFAAPRQDVGT
                     KIKVVPHINDSNEVRLELTEEISEQGAASGALGVVSITKRTAETTVVVRDQQTVVIGG
                     LMRDAVVSSESKVPVLGDLPLLGFLFRNSSNTKQKTNLLLILTPYIIRDQDDLRAIFE
                     RKMQERQEFLDRYFVFSDQKPYEPPKDFSRANGLVETIRQAQFQVQERKRLLEESRPD
                     AAKQHEPGVPIEWVPSSSGGGEQVARDPTSPPPAPHAPPAGGPPNRTRVAPIRVTPPA
                     KNVESPPNPEPR"
     gene            1046..2827
                     /gene="gspE"
                     /locus_tag="PK710_10555"
     CDS             1046..2827
                     /gene="gspE"
                     /locus_tag="PK710_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HOT10203.1"
                     /translation="MREDRFLGEVLVRHGLLSPERLEQLFELQREKGSDLIGLLVSGN
                     IVDEVSLHRALADEAGLPFVEKIDVEAIPTALATQLPITYAKAHRIIPIDETDSQITI
                     LCADPFSTATIDDVRALLSKRVDLKVATAEGIVDAINRVYEREVGHASDLKTESGEDA
                     DDDEIVDILDSDDDAPIIRWVRSLYRQAVKERASDIHIEPEEREVIVRYRIDGKLYVA
                     KKAPRQFMNSIISRVKIEAGLNIAEKRLPQDGRISFKIAGKSIDVRVSTIPTSRGFER
                     IVMRLLHKTAVLLDLTDLGFSSRDYALMDGLITKPNGIILVTGPTGSGKTTTLYACLN
                     RINKPNINILTAEDPVEYEIGGIHQVPVQAKIGLTFAAALRSFLRQDPDVIMVGEIRD
                     RETAEIAIHASLTGHLVLSTIHTNDAASAVTRLVEMGIEPFLVRSSVIGILAQRLVRV
                     LCPDCREAYHPSAYELSQLGIDPERSKARRARNISARYLPHGCDYVPVGTVEGQVPTV
                     YRPRGCPACMDTGYTGRKGIYELLMVNEFMAPLILRNADAQSIKQVAMERGMDSLRDD
                     GARKVLEGVTSVAEVLAATQEDVAVAE"
     gene            2832..4058
                     /gene="gspF"
                     /locus_tag="PK710_10560"
     CDS             2832..4058
                     /gene="gspF"
                     /locus_tag="PK710_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HOT10204.1"
                     /translation="MAVYEYRGIIIASGKATKGVRDADNPKQLRAMLRRDGVMLTSAT
                     EEAQAKAKKKREIDILAIFRRVNATDIAVMTRQLATLVRAGIPLVDAITALTEQVEKE
                     DLKRVLTNLRERLNEGTSFAQALDLHPKVFPPIFVNMVAAGEVSGTLEQVLERLADFL
                     ESQAQLKGKVSAALAYPALMLIIGTVLISVLMVAVVPKVTSIFESLDQALPWYTQVLI
                     AISDFLSGYWWLLLPTIALGVYLFNRWKKTKDGRLKWDTFTLKTPVFGPLLQMLAIAR
                     FAKTLSTLLSAGVPLLKAMEISNRVLTNALLERVVQDATGSIREGESIAVPLRRSGQF
                     PPMVTHMIAVGEKSGQLEEMLESVSKAYDAQVDTRVQMLTSLLEPLMIVLMGGSVGFI
                     AFAILMPLIQMNDFVK"
     gene            4077..4487
                     /locus_tag="PK710_10565"
     CDS             4077..4487
                     /locus_tag="PK710_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HOT10205.1"
                     /translation="MGKKRAKDRNIILPWEQRNAFFRRPGMSRARPVLMTIAVIVVFL
                     LIAQRERTASGVRATRASLLIVRRAVDTYRAEHGACPPDLMELERKNYIKQVPTDAWG
                     RPFLLTCPGRFDPAGYELSSAGPDGIPGGLDRIE"
     gene            4507..4917
                     /locus_tag="PK710_10570"
     CDS             4507..4917
                     /locus_tag="PK710_10570"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOT10206.1"
                     /translation="MHRSSRSFRLARAAARGVTLIEVLIVVAILSLIAAGVTVAVLPK
                     FRQAQIDTASTNVREIRGAVVRWRANRGGDECPTISQLVQDKEIDSASKTTDPWDSPY
                     KIECAEDETYVSSPGPDKKEGTKDDIIIPEKGNR"
     gene            4942..5652
                     /locus_tag="PK710_10575"
     CDS             4942..5652
                     /locus_tag="PK710_10575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOT10207.1"
                     /translation="MKTIARSRRHLDGFTLIELIVVVALMALFMGTIAFGMGAATNSR
                     VRGAATMIASGIRVAFSRSASTSKSLRAVFDLDQNSMLIEEADLPMLVRRDDVTGADG
                     ATGSTPQEQAAIQEAERLVKGPTAPRPLFKPITAFGFDAEDPSKGRELGRGVAFRRVE
                     VSHQEDPILSGRAYLYFWPGGQTERASIQVGRKGDTGDSGVISILVSPLTGRVRIETG
                     AKSMERPRDDSDREDRGF"
     gene            5652..6449
                     /locus_tag="PK710_10580"
     CDS             5652..6449
                     /locus_tag="PK710_10580"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOT10208.1"
                     /translation="MRDRGFTLLEVMVAVAILGLSLTVILSAQTGLYAAMTHAKNETV
                     AIGMARCKMGEIEEELLRLGYQETDVMEEGECCEDQTPARVRCKWKIERVELPDPPTF
                     DEMNPAGDGAGLDLSTPNTDLSSGMPGGAGALGVLANLGTSGGSALQSGGGIGALGSM
                     LGDASGGVAGLAPMVMSIVYPSLKPMLEASIRKISVTVLWKEGINDRELAVVQYVTNP
                     MRGGLGMGMEGAETDLLSGAFGPSESGATEPSGRTPRVSPMATSRGK"
     gene            6452..7153
                     /locus_tag="PK710_10585"
     CDS             6452..7153
                     /locus_tag="PK710_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HOT10209.1"
                     /translation="MGKRAIREAGRGVTLLEVIVSVAILAMVSLLIYGAFDGMAKGKR
                     GVEQINQRYREGRLAIRRLAREIPSAFLSAHQPYNQSLIVRETLFVGENTTPADRLDF
                     TSFSHRRVLRDSHESDQNEVSYFGSRDPDVPGKVDLARRESSLIDLEADRGGSVQVLA
                     EDIDLFDLEYLDPLSGLWQERWDSTSATGQLGRLPLQVKVTLVLRGGPSGQTIPFVAR
                     IALAMQSPLTFALPQ"
     gene            7150..8607
                     /locus_tag="PK710_10590"
     CDS             7150..8607
                     /locus_tag="PK710_10590"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOT10210.1"
                     /translation="MKHDRPIRCVTRLRSMRRRRSRRGVALIMVLAAITVLTVMLAEF
                     QDESSAELSGALSDRDALRAEYMARSGIQLARLLIAAEPTIRQAIAPLFLLMSGGKGT
                     APQIPVWEFSDRVLGAFNDSTGNEEFQSLAGVDLSQGRNLGIEGGRFELVIVDEDSKI
                     NVNMASRGDAFSQTRMAQQLLGLMAADQYKPMFEERDIDDQYSDALTICSAFIDWADP
                     DENYFNCDPRATVTASATAPEDSFYQMLKVPYRRKNAAFDSLEEIRMVRGVGDDFWAT
                     FVDPEPGNPKKRVMTVWGQGQVNVNTANAQTIWAIVCAGAPQATICVDPAQAQTFLTF
                     FSMAKGFTMGAPLFFSPKAFVSAMKGQGMVGPILTSLGIEPVQFLSEAETVKMVATES
                     KVFSIYADGVVPGYRRETRTRIHAVVDFRTAPAPGFGPGFGADGSAAGSMGNPPGSAA
                     GSSQFGADSSSLGEGAIQGAVAPNPGGTIIYWRME"
     gene            8619..>9923
                     /gene="pilM"
                     /locus_tag="PK710_10595"
     CDS             8619..>9923
                     /gene="pilM"
                     /locus_tag="PK710_10595"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOT10211.1"
                     /translation="MANLLGIDIAEDSVRVAWLRTGYRSISVVALEQQPIASYASVAD
                     AVRAVVGPMLSRGDSIATVLLGDKLFIRKVELPPTAMRQVAEVLPFELEALLPFDLED
                     AIFDSRVLPRQGPKAPIPILACAARLEQVKERIELVQQALSAEPERVDASSIALANLA
                     ALIPELAVPGPVMLVHLDGWTSDIAVLNAGKVEYVRTVSSGTEGLPASAKELARELRQ
                     SLFAWRSQGGAQPQVVYLTGPGSTLPGAEAYLSSELGVQMMALPAPRMEGMTPELQQK
                     LPSMMRAVALAMGLTRTQASVNLRQGPLSYERGFSYLQEKIPLLVGIFFLIFVSFLFS
                     TWMEWRSLSKQTDVLEEALAMVTKQVVGEAIRDPEMAEDAVSVTTSKADDPMPTVDGF
                     DVMIEISKAVPEDMKHDIEELDYQKGKVTIHGIVSTIPEAQQI"
BASE COUNT         2066 a         2392 c         3278 g         2187 t
ORIGIN      
        1 gggaccacca acttagtggc gactggattg acgggtcccg cgtgtggtgt ggtggtcaat
       61 gcgttagcga cgagcagtga tgcgaacgta cttgccacgc cgcatatcat agcgacggac
      121 aacgtgcctg ccgagatcag cgtggggcag aacataccgc ttcagacgaa tttgggggga
      181 ttgggtagca tgggtagcct tgcggggttg gcgggaggtc aggcgggatc ggccgcgtcg
      241 agccttggcg cgttgagcag cttcggtttc ggaggttttg cggcgcctcg ccaagacgtg
      301 ggcacgaaaa tcaaagtggt gccgcatatc aatgattcca atgaggtacg cctcgaattg
      361 accgaagaga tcagcgagca aggagcggct tcgggcgcgt tgggtgttgt ctcgattacg
      421 aagcgaacgg ccgagacgac cgtggtggtt cgcgatcagc agaccgtggt gattggcggt
      481 ttgatgcgcg atgcggtggt atcgagcgag agcaaggtgc cggtgctcgg ggatctaccc
      541 ctgcttggat ttttgttcag aaactcgagc aataccaagc agaagaccaa cttgctgctg
      601 atcctcacgc cgtacatcat tcgcgatcaa gacgatctgc gagcgatctt cgaacgaaaa
      661 atgcaggaac gtcaggagtt tttggatcgt tacttcgtat ttagcgacca gaagccgtac
      721 gagccgccga aagacttcag tcgggccaac ggattggtgg aaaccattcg tcaggcgcag
      781 ttccaggtgc aggagcgaaa acggctgctg gaagagagcc gtcctgatgc ggccaaacaa
      841 cacgagccgg gagtgcccat cgaatgggtg ccttcgagtt ctggaggcgg agagcaagtc
      901 gcgcgcgacc caacttcgcc ccctcccgcg ccccatgctc caccggccgg tggacctccc
      961 aatcgaacgc gggttgcgcc catccgggtc acccctcccg cgaaaaacgt ggaatctcct
     1021 ccgaaccccg agccaaggta gccgcatgcg tgaagatcgt tttcttggcg aggtgctggt
     1081 tcgacatggg cttctgtctc ccgagcggct cgaacagctc ttcgaactgc agcgcgaaaa
     1141 aggctccgat ctcatcgggt tgctcgtctc gggcaatatc gtcgatgagg tgtcgctgca
     1201 tcgcgcgctg gccgacgaag caggacttcc tttcgtcgaa aaaatcgatg tggaagccat
     1261 tcccacggct ctcgccacgc agttgcccat cacctacgcg aaagcccatc gaatcattcc
     1321 catcgacgaa acggattcac agatcacgat tttatgcgcc gatcctttca gtaccgccac
     1381 gatcgatgat gttcgagccc tgctgagcaa acgagtagac ctgaaggttg cgacggccga
     1441 agggatcgtg gatgccatca atcgggttta tgaacgagaa gttggacacg ccagcgatct
     1501 caaaaccgag agtggagaag acgcggacga tgatgaaatc gtcgatattc tcgattcgga
     1561 tgatgatgcg cccatcattc gttgggtgcg gtcgttgtat cggcaagcgg tgaaggagcg
     1621 tgcgagcgat attcatatcg agccggaaga gcgggaggtg attgttcgtt atcgtatcga
     1681 tggcaagctt tacgtggcga aaaaggctcc gcggcaattc atgaactcga tcatatcgcg
     1741 tgttaagatc gaggcggggt tgaatatcgc ggagaagagg ctcccgcagg atggccgtat
     1801 ttccttcaaa atcgctggta aaagcatcga tgtgcgcgtg tcgacgattc ccacgagccg
     1861 cggcttcgaa cgaatcgtca tgcgactatt gcataaaacc gccgtgttgt tggacctcac
     1921 ggacctgggg ttttcctcga gggattatgc gttgatggac gggctgatca ccaagcccaa
     1981 cggcatcatt ttggtgacgg gaccgaccgg tagtggcaag acgaccacgc tatatgcttg
     2041 tttgaatcgt atcaacaagc cgaatatcaa tatcctcacc gccgaggatc cggtggagta
     2101 tgagatcggc ggcatccatc aggtgccggt acaagcgaag attgggctga ctttcgccgc
     2161 ggcgttgcgg tcttttcttc gtcaggaccc ggacgtcatc atggtggggg aaatccggga
     2221 ccgggaaacc gcggaaatcg ccattcacgc ctccttgacg gggcatctcg tgttgtcgac
     2281 gatccatacg aatgacgcgg ccagtgcggt cacgcgtctg gtggaaatgg ggattgagcc
     2341 gtttttggtg cgttcgagtg tgatcgggat tctggcgcag cggctcgtgc gggtgctatg
     2401 ccctgattgc agggaggcat atcatccctc ggcctacgaa ttgagccagt tgggaatcga
     2461 ccccgaacgc agcaaggcgc gtcgtgcccg caatatatct gcccggtatc taccgcatgg
     2521 ttgtgattat gtgcccgtgg gaaccgtgga agggcaagtg cccacggtgt atcggccccg
     2581 aggatgtccc gcgtgtatgg ataccggcta tacgggaaga aaggggatct atgagctgct
     2641 gatggtcaac gaattcatgg cgcccttgat tttgcgcaat gccgatgcgc aatcgatcaa
     2701 gcaggtggcg atggaaaggg gcatggattc gttgcgagac gatggggctc gcaaagtgtt
     2761 ggaaggtgtg acgtcggtgg ccgaggtatt ggcggccacg caagaagacg tggccgtggc
     2821 ggagtaaggc gatggcagtc tacgaatacc gcggaatcat cattgcctcc ggaaaggcga
     2881 ccaagggggt ccgcgacgct gacaatccga agcagcttcg ggcgatgctg cgtcgcgatg
     2941 gggtgatgct gacctcggcg acggaggagg cccaggccaa ggcgaaaaag aagcgtgaaa
     3001 tcgatatctt agcgattttt cgtcgggtca acgcgacgga tatcgcggtg atgaccaggc
     3061 aattggccac gttggtgcga gcggggatcc ctttggtgga tgcgatcacg gcgctgacgg
     3121 agcaggtgga gaaggaggat ttgaagcgtg tgctcacgaa ccttcgagag cggctcaacg
     3181 agggaacgag ttttgcgcag gctttggatc tgcatcccaa ggtatttcca ccgattttcg
     3241 tgaacatggt ggcagcgggg gaggtgtctg ggacgttgga gcaggttctc gagcgtttgg
     3301 ccgactttct cgagagtcag gcgcaactca agggaaaagt atcagcggcg ctggcgtatc
     3361 cggcgctcat gctgatcatt ggaaccgtgc tcatcagcgt attgatggtg gcggtggtcc
     3421 cgaaggtgac gagtattttc gaaagcctgg atcaagcctt gccttggtac acccaggtgc
     3481 tgattgccat ctcggacttt ttatctgggt attggtggct gcttttgccc acgattgcgc
     3541 tgggggtcta cctctttaat cgatggaaaa aaacgaaaga cggacggttg aagtgggata
     3601 ctttcacgct caagacgcct gtattcggtc cgcttcttca gatgcttgcg attgcgaggt
     3661 ttgcaaaaac gttatcgacg ctcttgagtg cgggggtgcc tttgctcaag gccatggaaa
     3721 tttcgaatcg ggtgttgacg aatgctttgc tagagcgtgt ggtgcaagac gcgacggggt
     3781 cgattcgtga gggggagagc attgccgtgc ctcttcgaag aagtggccaa tttccgccca
     3841 tggttaccca catgattgcc gtgggggaga aaagcgggca gctcgaagag atgctcgaaa
     3901 gcgtatccaa agcctatgac gcgcaggtcg atacccgtgt gcagatgctt accagcttgt
     3961 tggaaccatt gatgatcgtg ttgatgggag gttccgtggg gttcatcgca tttgcgattt
     4021 tgatgccctt gatccagatg aatgacttcg tgaagtaatg gccgaggcga tgaaaaatgg
     4081 gaaaaaagcg tgcgaaggac agaaatatca tcctgccctg ggagcagcga aacgcttttt
     4141 tccgtcgacc gggcatgtct cgggctcgtc ccgtgttgat gacgattgcc gtgatcgttg
     4201 tatttttgct cattgcccag cgcgaacgaa ctgcctcggg agtacgcgcc acgcgagcgt
     4261 cgcttttgat agtgcggcgt gcggtggata cgtatcgggc cgaacatggc gcgtgcccgc
     4321 cggacctgat ggaactcgag cgcaaaaact atatcaaaca ggttcctacg gatgcgtggg
     4381 gcagaccttt tcttttgacc tgtcccggcc gcttcgatcc ggccggatac gaattgtcct
     4441 ccgcgggccc cgatggaatt cccggaggct tggaccgaat cgaataacgc catggagatg
     4501 aatgacatgc atcgtagttc tcgctcgttt cgtctggctc gcgccgccgc ccgcggtgtg
     4561 actctcatcg aagtcctgat tgtcgttgcg attctcagcc tcatcgccgc gggtgttacc
     4621 gttgcggtgc ttcccaaatt ccgtcaggcc cagatcgata ccgcgagcac caacgtccgt
     4681 gaaattcggg gggcggtcgt tcgatggcgc gccaatcgcg gaggcgatga gtgtccgacg
     4741 atttctcaac tcgtgcagga caaggagatc gacagcgcgt cgaagaccac cgatccgtgg
     4801 gattcgccct ataaaatcga atgtgcggaa gacgagacct acgtgtcttc ccccggaccc
     4861 gacaagaaag aagggaccaa agacgacatt atcatacctg aaaaagggaa tcgataatcg
     4921 gaactgaacg atgggaggcc tatgaaaacg attgccagaa gtcgacgaca tcttgatggg
     4981 ttcacgttga ttgaactcat cgttgtggtg gctctcatgg cgttattcat gggcacgatc
     5041 gcttttggca tgggggcggc tacgaattcg cgggttcgtg gcgccgccac gatgattgcc
     5101 agtggaatcc gcgtggcttt ttctcggtcc gcatccacct ccaagtccct acgcgcggta
     5161 ttcgacctcg accagaattc catgctgatc gaggaagcgg atttgcccat gttggttcgc
     5221 cgcgacgacg tgaccggtgc ggatggggcc acgggcagca caccgcaaga gcaggcagcg
     5281 attcaagaag ccgagcggct cgtcaagggg ccgacggctc cacgtccgtt gttcaagccc
     5341 atcacggctt ttggcttcga tgccgaggat ccgtccaaag gccgtgaatt gggacgcggt
     5401 gtggcgtttc gtcgtgtgga agtatcgcat caggaggatc cgatcctatc ggggcgggcg
     5461 tacttgtatt tttggccggg agggcaaacg gaacgagcgt cgattcaggt ggggagaaag
     5521 ggagatacgg gtgattcggg ggtgatttcg atcctcgtat caccgttgac ggggcgtgtt
     5581 cgtatcgaaa cgggggcgaa gtccatggag cgaccgaggg atgatagtga cagagaggac
     5641 agggggtttt gatgagggac cggggcttta cgctgctgga agtcatggtg gcggtggcca
     5701 ttcttggctt gagtctcacg gtgattttat cggcgcaaac ggggctgtat gcagccatga
     5761 cccacgcaaa aaacgaaacg gtggccattg ggatggcccg ttgcaagatg ggggaaatcg
     5821 aggaggaact cctgagactg ggctatcagg agacggatgt gatggaggag ggggagtgtt
     5881 gtgaagacca gactcctgcg agggtgcggt gcaagtggaa gatcgagcgg gtggaattgc
     5941 cggatcctcc cacgttcgac gagatgaatc ccgcggggga tggggcgggg ttggatcttt
     6001 ccacgcccaa tacggacttg tcttctggga tgcctggggg agcgggggcg ctgggggtgc
     6061 tggcgaactt gggtacgtca ggcggtagtg cgctccaatc tggggggggg attggagcgt
     6121 tgggttcgat gcttggggat gcttccggag gggtagcggg gcttgcgccc atggtcatgt
     6181 cgattgtgta tccaagtctc aagccgatgt tggaggcgag cattcgaaaa atatccgtaa
     6241 cggtattgtg gaaagaggga atcaacgacc gggaactggc cgtagtgcag tatgtgacca
     6301 atccgatgcg tggcgggttg ggcatgggaa tggaaggtgc ggaaacggat ctgctctcag
     6361 gagcttttgg tccttctgaa tccggggcga cggagccatc ggggcgaacg ccgcgtgtgt
     6421 ctcccatggc gacgagcaga ggcaagtagg catgggaaag cgggctatca gagaggctgg
     6481 acgcggtgtg acgttgctgg aagtcatcgt atccgtggcc attctcgcga tggtatcgct
     6541 gctgatttac ggggcgttcg atggcatggc gaagggaaag cgcggagtgg agcagatcaa
     6601 ccagcggtat cgagaagggc ggttggcgat tcgtcgtttg gcgagagaga taccgagcgc
     6661 ctttctttct gctcatcagc cctacaacca gagtctcatc gtgagggaga cgctatttgt
     6721 gggagaaaat actacaccag cggatcgtct tgattttact tcattttctc atcgacgggt
     6781 gttgcgagac tcgcatgaat cggatcaaaa cgaagtatcc tattttggct cgagggatcc
     6841 ggatgtgcct ggcaaggtag atttggcgcg aagagagtcg agtctcatcg atttggaggc
     6901 ggatcgaggg ggaagcgtgc aggtgctggc ggaggacatc gatttattcg acctcgagta
     6961 tctcgatccg ctatcgggct tatggcaaga gcgttgggat tcgacctccg cgacggggca
     7021 actcgggcgt ttacctttgc aggtcaaggt gacgttggtg ctgcgtgggg ggccgagcgg
     7081 tcagacgatt cctttcgtgg cgcgaattgc tctcgccatg caatctcctc ttacgtttgc
     7141 gttaccccaa tgaaacacga tcgtcccatc cgttgtgtga ctcgtcttcg ctcgatgcga
     7201 aggcgtcgtt cgcgacgagg tgtggcgttg atcatggtgc ttgcggcgat cacggtattg
     7261 accgtgatgc ttgcggagtt tcaggatgag tcttcggcgg agttgtctgg ggcgttatcg
     7321 gaccgggacg cgcttcgggc ggagtatatg gcgcgcagtg ggattcaact ggcacgtttg
     7381 ttgattgcgg cggaaccgac gattcggcaa gcgattgcgc cgctttttct gttgatgtcc
     7441 gggggcaagg ggacggctcc gcagataccg gtgtgggagt tttccgatcg ggtgcttgga
     7501 gcgttcaacg acagcaccgg aaacgaggaa tttcagtctc ttgcgggagt tgatctcagt
     7561 caggggcgca atctgggcat cgaaggcggt cgtttcgagc tggtaatcgt cgatgaagac
     7621 tccaaaatca atgtgaatat ggcatcacgg ggggatgctt tcagccagac acgcatggcg
     7681 cagcagcttt tgggtttgat ggcggcggat caatacaaac cgatgttcga ggagcgggac
     7741 attgacgatc agtattccga cgccttgacg atttgttcgg cattcattga ttgggcggat
     7801 ccggatgaaa actacttcaa ctgtgatccg agagcgacgg tgacggcctc ggccacggcg
     7861 ccggaggact ccttttatca gatgttgaag gtgccttatc gtcgcaagaa cgcggctttc
     7921 gacagtttgg aggagattcg gatggtgcgg ggggtagggg atgatttttg ggcgacgttc
     7981 gtggatccgg agccgggaaa tccgaaaaag cgtgtcatga cggtgtgggg gcaggggcag
     8041 gtcaatgtca acacggcgaa tgcgcagacg atatgggcga ttgtatgtgc gggggcgcct
     8101 caagccacga tttgtgtaga tccggcgcaa gcgcagacgt ttttgacatt ttttagtatg
     8161 gcgaagggat tcacgatggg agccccatta tttttttcgc ccaaggcatt tgtgagtgcg
     8221 atgaaggggc aggggatggt ggggccgatt ttgacgagtt tggggattga gccggtgcag
     8281 tttttatcgg aagccgagac cgtgaaaatg gtcgccaccg aaagcaaggt tttcagcatt
     8341 tatgccgatg gggtggtacc gggctatcgt cgtgaaacac ggacccggat ccatgccgtg
     8401 gtggactttc ggacggcgcc ggcgccagga ttcggccctg gtttcggtgc ggatggcagc
     8461 gcggcgggtt ctatgggaaa tcctccgggt tctgctgccg gctccagtca atttggagcg
     8521 gattcctcat ccttgggtga gggagccatt cagggggccg tggcacccaa cccggggggg
     8581 accatcatct actggcgaat ggagtaggaa cagacagcat ggccaacctt cttggcattg
     8641 acatcgcgga ggatagcgtt cgagtcgcat ggctacgaac tggctaccga agcatctccg
     8701 tggtggctct cgagcagcaa ccgatagcga gttatgcatc ggtggcggac gcggtgcggg
     8761 cggtggtagg gccgatgctg tctcggggag actcgatcgc cacggtattg cttggtgaca
     8821 agttattcat tcggaaggtc gagttgccgc ccacggcgat gcgacaggtg gcggaggttc
     8881 ttcctttcga attggaggcg ttgcttcctt tcgatctgga ggatgcgata ttcgacagtc
     8941 gtgtattgcc gcgacagggg cccaaagctc cgattcccat ccttgcttgt gcggcgaggc
     9001 tcgagcaggt caaagaacga atcgaactgg ttcagcaggc gttgagcgcg gagccggagc
     9061 gtgtggacgc ttcgagcata gcgttggcga acctcgccgc gttgattcct gaactggcgg
     9121 ttcccgggcc tgtcatgctc gtccatctcg atgggtggac gagtgatatt gcggtgctca
     9181 atgccggaaa agtcgaatat gtgcgcacgg tatcctccgg aaccgagggg cttcccgctt
     9241 ccgccaagga gcttgcgcgg gagttgcggc aaagcctttt tgcctggcgt tcccaagggg
     9301 gagcgcagcc acaggtcgtt tatctgacgg gaccgggatc gacgctgccg ggagcagaag
     9361 cctatctttc ctcggaactg ggggttcaga tgatggcgtt gccggcgccg cgcatggagg
     9421 gaatgacgcc ggaactgcaa caaaaactgc cttccatgat gcgtgccgtg gcccttgcca
     9481 tgggattgac gcgtacccag gctagtgtga acttgcgtca ggggcctctg tcttacgagc
     9541 ggggcttcag ctaccttcaa gaaaaaatcc ctttgttggt gggtatcttt ttcctcattt
     9601 ttgtgagttt tttgttctct acgtggatgg aatggcgctc gctcagcaaa caaaccgacg
     9661 tgttggaaga agcgctggcg atggtgacga agcaagtcgt cggcgaggcg attcgcgatc
     9721 cggaaatggc ggaagatgcg gtgagtgtga ccacttcgaa agccgatgat ccgatgccga
     9781 ccgtcgatgg cttcgatgtg atgatcgaga tttcgaaagc ggtgccggag gacatgaagc
     9841 acgatatcga agaactcgat taccaaaaag ggaaggtaac gattcacggg attgtcagca
     9901 ccatcccgga agcgcagcag att
//
LOCUS       DAOSOR010000162         9910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6804_length_9910_cov_3.493252, whole genome shotgun sequence.
ACCESSION   DAOSOR010000162 DAOSOR010000000
VERSION     DAOSOR010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9910
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6804_length_9910_cov_3.493252"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="PK710_10600"
     CDS             complement(<1..1181)
                     /locus_tag="PK710_10600"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOT10212.1"
                     /translation="MRIRDIALVCCVSVFGCKDPAASVSSEASTISPAQPTVAAAPSA
                     STADSGKPSTVSKINRVVLITIDALRGDQPWTGYTLAKTPVLSEFAERGIVYTRNYAI
                     ANTTGPAISGLLAVRYPTETDRDNCPLAGFTIGEGVGPVLSSAGVWTGAAHGHAYFGG
                     STAPKGGFEDWRVIDNVGGKMATRGAVTGPEVTELMLALLKDAPTDKPSFLWAHYLEP
                     HDSYVYHKDFPPSSDPRRGVYDGEVAFVDDQIGKVLAAIDASDMKDRTAVIVSADHGE
                     AFGEHERYRHGFTVFEEEVRVPLMLRIPGQAAKRIEVPRSSMDIPRTIAALLNVPAPA
                     RWRGHSLLEDLGTAPPPQRPVIIDVPELMNLPALQAVVMDKIKVIKSGPKWIVYDLEA
                     DP"
     gene            complement(1448..4345)
                     /locus_tag="PK710_10605"
     CDS             complement(1448..4345)
                     /locus_tag="PK710_10605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HOT10213.1"
                     /translation="MRKNYIFTGLVATLALTSVGVASAEPKLRIQVDQRGDFLLIGNT
                     MGHECLSGTPTPVKGAIDTACSSQTAAQRNDSSPDAFWNNDTLMGEAGKVSDARTTAY
                     LTIPSGATVTHAYLYWAGAQTTVDNEVQVRFGTETLPTTITATDSSTITVSYAAYQAV
                     ADVTDLVQAKGSGPYEVSGIATPEFYGVNNDVVFGGWSMVVLYRLATEPPRNLAVFDG
                     LERVSSGNNRTVKLSGFLVPNAGFDGKLGIVAYEGDASLTGDRLFFHSTSTVPPNNQA
                     LSTTNNPIDNFFNGTRTWLGDLVSNVGDLPQTTGGPQSFVGMDHDVVDVRSKLTAGQT
                     EAWVTATTNGDVYFPGIWVTSISTFKPDFTTSEKTVTDDNGGAVMVGDILTYTITVKN
                     TGNDIAVNTVLNDPLPDGVEFYGTSITVDGVARTAAVDTDVAEYDAAQHLVTVRLGDG
                     ATDTAGGDLGIGESSTISFKVRIKPGSTGLLENQAEITAEGQQGAPETTTPTDGNGTQ
                     PGTPPTGIHIDECATDADCDGSLLCNTASTPNTCVECLADNDCGGPNSGQLCDSNTHT
                     CIEGCNSETDNGCPSGLHCTSQGLAIGQCVACVEDNHCGGPNSGQLCDLDTYTCFAGC
                     NGEKDNGCPAGKVCTSDDDTVGSCVECVVHNDCGGPNSGQLCSSTNTCVAGCTGNVGE
                     GCPDPLVCSSSDSTVGSCVGCMTDSQCGAADSGKICHDTDHACVDGCRGENGNGCASG
                     LTCSSTDSSMGTCAQCFKDSDCGALDSGKICHDETKACIDGCRGEDGNGCPSGLTCTS
                     SDKTMGICAECMTDSDCGAVDSGRVCHDTTKVCVDGCRGEEGNGCPSGEECTSTDTSI
                     GTCEGTGGTGGSGGAGGSSGTGGEAGTGGGTAGTGGGTAGTGGTAGSGGTSGTGGKGG
                     STVFNAENYAAEGNGILCAASPAKRNGSETGWLAGLFALGAVAVMRRRRHS"
     gene            complement(4364..6175)
                     /locus_tag="PK710_10610"
     CDS             complement(4364..6175)
                     /locus_tag="PK710_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT10214.1"
                     /translation="MALRLKSIVTMGVLGASWFATGHEARADGLPLNRFDPAPMGDRM
                     FGVQSPYVAGHLTPHVGLILDYAHNPLVLRNTETDDKTGNIVTGQLFAHLNGTLPLFE
                     RLHLNVDVPFALYQDGENPSEAGSLPSASGAQMGEVRAGLRLRLLGEYHDAFQLGLAG
                     YAWLPTGPSDGYVGNGKVRGMPQLVVGGRSESVVWSAAAGPQFRAGQSFANVSDGTQL
                     NAGAGVGFLFGDKKQFQVGPEVYAAFTLSTDDKALHDDAARNSNVELLLDGRWRFLEH
                     FEAGLGVGPGLTTGLGTPSFRGVFMLGFTPSQEKAAGPYDTDGDGITDDLDACPNVPG
                     VPSDDPAKHGCPADRDGDGILDINDACPDVPGVATDDPKTNGCPPDRDGDGIIDSLDA
                     CPDVPGVATDDPKTNGCPPDRDGDGIFDAQDACPDVPGVASDDPQKNGCPPDTDGDGI
                     IDSEDACPTEAGPRDPDPKKNGCPTVHVTDKEIVILEQVQFDTNKATIRAVSDELLDK
                     VAAVFKDHPEILKVEVQGHTDNTGAPGYNKGLSQRRADAVVAALVKRGIDRARLVGKG
                     YGQEQPIADNGTDEGRQKNRRVQFQIIERQPKTEGTK"
     gene            complement(6442..7668)
                     /locus_tag="PK710_10615"
     CDS             complement(6442..7668)
                     /locus_tag="PK710_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOT10215.1"
                     /translation="MWACKVDSTGLGESGMYKIQTLNNISAAGLEKLPKDRYVVASQM
                     DGPDAILVRSAKMHDLEIPDSVKAIGRAGAGTNNIPVSLMTARGIAVFNTPGANANAV
                     KELVVASLLMAARNLGSGWLFARGLEGSDAEISKAVEAGKKQFSGFELPGRTLGVVGL
                     GAIGVKVANVARALGMRAVGYDPTITVQRAWQLDTDVLSAGSIEELLGLSDMVTVHVP
                     FSDETRHLIDATRIKAMRPGTVLLNFSRAGIVDDTAVVAALNEGHLHAYVCDFPSNLL
                     KNHPKVVTFPHLGASTKEAEDNCAIMVADQVREYLEHGTVNNSVNFPNAFLPRNSGYR
                     MAIVNSNVPNMVGQISTELAAVGLNILDMLNRSRGEIAVTLIDLDKPCPEERIEKIRN
                     ISGVLSVRCLGEEAHP"
     gene            complement(7780..8463)
                     /locus_tag="PK710_10620"
     CDS             complement(7780..8463)
                     /locus_tag="PK710_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10216.1"
                     /translation="MKRAGDSGRYGCCIRWSLWVAAWCAGMTAWLGGIHPADAQIKQP
                     GTHLRYGVEVEPHLLFMHDGHSWYGDDDGWGPGVRLSVPIIEDGPINTINNSLAIGFG
                     LDWAHFKGHCWSPAWRVRPSPNDPYYHYWYEKCSANEIWVPVVLQWNFFFTDFVSAMA
                     ELGPSIVRRSWEYYVPCPGGWCKDTEADLDLEPLIFHAGVRLHFSQSVGMTIRLGWPY
                     LSAGASLFF"
     gene            complement(8722..9354)
                     /locus_tag="PK710_10625"
     CDS             complement(8722..9354)
                     /locus_tag="PK710_10625"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOT10217.1"
                     /translation="MRWMIGFLSVMGVCAAGCDRRPEGALVDRPRAPLRTVGSNHGPS
                     SEAGGRESANRCMIPTPQQGPPPVGPAESCPIDPVFGGVTLEKAQVSFPQAQDAPVLE
                     VELARSPRERQRGLMYRTHMAENAGMLFDFPGRPEVQSFWMRNTCIPLDMLFIEEDGF
                     IAGIVENVPTLNEASRSVPCPVRYVLEVNAGWTRRHGVRAGQWVELPTSD"
     gene            complement(9454..>9910)
                     /locus_tag="PK710_10630"
     CDS             complement(9454..>9910)
                     /locus_tag="PK710_10630"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOT10218.1"
                     /translation="GPAHRVVIPEGFYFGLGINNCLAVDFHGHVNSGGRFSNHHSGIG
                     GGAIIWRGLSRGGIGYLCLKSTYRGLDGSQRSSIVPFMPEGTPISHVGPDLMGGREGA
                     RFYLVTEHGVARISGNSQSGFIRAVISVAHPDHRDELRFAAWEHFRVRV"
BASE COUNT         2217 a         3231 c         2412 g         2050 t
ORIGIN      
        1 gggtccgctt cgagatcgta cacgatccac ttggggccgg acttgatgac tttgatcttg
       61 tccatcacta cggcttgaag ggcgggcagg ttcatcagtt ccggtacatc gatgatcact
      121 ggacgttgtg gcggcggcgc tgtccccagg tcttccaata gcgaatgacc ccgccacctt
      181 gccggcgctg gcacattcag cagcgcggca atcgttcgcg gaatatccat gctcgaacgg
      241 ggtacctcga ttcgcttggc cgcttgtccc ggaatccgaa gcatcagcgg caccctcacc
      301 tcctcttcga ataccgtaaa tccatggcga taacgctcat gctccccaaa cgcctcccca
      361 tggtcagcgg atacgatgac agccgtacga tctttcatat ccgatgcgtc gatggcggca
      421 agcactttgc caatctgatc gtccacgaag gctacttcac cgtcataaac gccgcgccga
      481 gggtccgaac tcggcggaaa atccttgtgg taaacgtagg aatcatgcgg ttcgagataa
      541 tgggcccaca aaaacgacgg cttgtccgtc ggcgcatctt tcaaaagagc aagcatcaac
      601 tctgtcacct ccggccccgt caccgctcct cgtgtcgcca tcttcccacc gacgttgtcg
      661 atgacacgcc agtcttcaaa tcctcccttt ggcgctgtag agccgccaaa ataggcgtga
      721 ccgtgagcag cccccgtcca tactcccgcc gaggacaaca cgggccccac cccctcgcca
      781 atcgtaaagc ccgcgagcgg acagttgtcc cgatccgttt ccgtaggata gcgcaccgca
      841 agaaggcctg aaatcgcagg tcccgtggtg ttggcgatcg catagttgcg cgtatacacg
      901 atgcctcgtt cggcaaactc cgaaagcaca ggcgtcttgg caagcgtata ccccgtccac
      961 ggttgatctc cacgcaacgc atcgatcgtg attaaaacca ctcggttgat tttcgaaacc
     1021 gtcgaaggtt ttccggaatc ggcagtcgat gcgctcggag ccgcggcaac cgtgggctgg
     1081 gcaggagata tcgtagaagc ctcggaagat accgaagcag cagggtcttt gcacccaaag
     1141 acgctcacgc aacacacaag ggcgatatct cggatacgca tgaaagccga tgtagcaaga
     1201 catgccaagg gcgtcacttg caaatcgagg aaacgagcgt tctatgcgcg atggctcgaa
     1261 ccatcagcag caatggcatc cacgcatgcg cgtggcgtga acgttcggat cacgtttgca
     1321 gatgtttcgt gcgatcctat cgcatggcgc aagctatccc gatagggtca atatattcac
     1381 atggaggagg ttgatgagga gattgacagg cacacgctcc tttggcgcat gcccatcatt
     1441 cgacttctta cgaatggcgg cgacgacgca tcacagcgac agcaccgaga gcgaagagac
     1501 ccgcgagcca gcccgtttcg gaaccattgc gcttcgcggg gcttgccgca cacaggatac
     1561 cgttgccttc ggccgcgtaa ttttcagcat tgaagaccgt ggatccaccc ttgccacccg
     1621 tgccggacgt tccacctgaa ccagcagtgc caccggttcc agccgttcca ccaccggttc
     1681 cagccgttcc accaccggta ccagcttctc cgccagtgcc ggaagaaccg ccagcgccac
     1741 cggatccacc cgtgcctcca gttccctcac aggtcccgat gctggtgtcc gtcgacgtac
     1801 attcttcgcc agaaggacag ccattgccct cttccccacg gcaaccatcc acacagacct
     1861 tggtggtatc gtggcaaacc cggccgctat ccacggctcc gcaatcgcta tccgtcatgc
     1921 actcggcgca gatacccatc gttttgtccg acgacgtgca ggtgagtccc gacggacaac
     1981 cattgccgtc ttctcctcgg caaccgtcga tgcaggcctt ggtttcatca tggcaaatct
     2041 tgccgctgtc caacgctccg caatcgctat ccttgaagca ttgcgcacac gttcccatgc
     2101 tgctgtccgt cgaagagcac gtcagacccg atgcacaacc gttgccattc tctcctcggc
     2161 aaccatccac acaggcatgg tccgtatcat ggcaaatttt gccactgtcc gccgcaccgc
     2221 attggctatc cgtcatgcat cccacgcaag atcccaccgt ggaatcactc gaggaacaca
     2281 caagcgggtc gggacatcct tccccaacgt tgcccgtgca accagcaaca catgtgttcg
     2341 tcgaactgca aagttgcccg ctgttcggtc ctccacaatc gttgtgaacc acacattcga
     2401 cgcaagaacc caccgtgtcg tcgtccgaag tgcatacctt gccggcggga caaccattgt
     2461 ctttttcgcc gttgcatccc gcaaaacaag tgtacgtgtc gagatcacat agctgtccgc
     2521 tattcggacc accgcaatgg ttgtcctcca cacaggcaac gcactgtccg atcgccaagc
     2581 cttgggacgt acaatgcaat ccactgggac aaccgttgtc cgtctcgcta ttgcatccct
     2641 cgatgcatgt gtgagtgttg gaatcacaaa gctgtccgct attcggaccc ccgcaatcgt
     2701 tatcggcaag acactcgaca caggtattgg gtgtgctcgc cgtattgcac agcaagctcc
     2761 catcgcaatc cgcatcggtc gcgcattcgt cgatgtgaat acccgtggga ggcgttcccg
     2821 gttgtgtacc gttaccatcg gtaggcgtcg tggtttcagg agccccttgc tgcccttccg
     2881 cggtgatttc cgcctggttt tcgagcaatc cggtcgaccc gggcttgatt ctaaccttga
     2941 agctgatcgt ggaagattcg ccaatcccca agtcaccacc agccgtgtcc gttgcgccat
     3001 cgcccaaccg aaccgtcacc agatgctggg ccgcatcata ctccgccaca tcagtatcga
     3061 ccgccgccgt tcttgcaaca ccatccacgg tgatcgaggt tccgtaaaac tccacgccgt
     3121 cgggcaacgg atcgttgagc accgtattca ccgcaatgtc attaccggtg tttttcaccg
     3181 tgatggtata cgtcaggata tcgcccacca tgaccgcgcc accgttgtcg tcagtcacgg
     3241 ttttttccga tgtggtaaag tccggcttga acgtggaaat cgacgttacc catatccccg
     3301 ggaagtagac gtcaccattg gtcgtcgcgg ttacccaagc ttccgtttgt cccgcggtaa
     3361 gcttagacct cacatcgacg acatcatgat ccatgcccac gaaactctga ggtcctccgg
     3421 tggtctgcgg aagatcgcca acgttgctca ccaagtctcc tagccaggtt cgagttccgt
     3481 tgaagaagtt gtcgatggga ttattcgtcg tcgacaacgc ctggttattc ggtggcaccg
     3541 tggacgtcga gtggaagaaa agtcgatcgc cggtcagact ggcgtcccct tcgtaagcaa
     3601 cgatgcctaa tttcccatcg aaaccggcgt tgggaaccaa aaagccgctc agcttgacgg
     3661 ttctgttgtt tcccgaggac acccgctcga gtccgtcgaa tactgccaaa ttgcgcggag
     3721 gctccgtggc aagccgatac agcacgacca tcgaccaacc accaaaaacc acgtcattgt
     3781 ttaccccgta gaactcgggt gtcgcaatgc cacttacctc ataaggaccg ctacctttgg
     3841 cctgcaccaa gtcggtcacg tcggcaaccg cctgataggc tgcatatgat acggtgattg
     3901 tcgacgaatc cgtggctgtg atggtggtgg gcagcgtctc cgtaccgaaa cggacctgca
     3961 cctcattgtc gacggtagtc tgagcgcccg cccaatacag gtacgcatgc gtcacggtcg
     4021 cgccgctggg aatcgtgaga taggcggtgg tgcgcgcatc actcactttc cccgcttcgc
     4081 ccatcaaggt gtcgttgttc caaaacgcat ccggcgaact atcgtttctt tgagctgccg
     4141 tttgcgaaga gcaagccgta tcaatcgctc ccttcaccgg agtgggggtt cccgataggc
     4201 actcatggcc catcgtattg ccaatgagca aaaaatcgcc ccgttgatcc acttgtatcc
     4261 gcagtttcgg ttccgcggag gcaaccccaa ccgacgtgag ggccaaggtc gccacaaggc
     4321 ctgtgaaaat ataattcttt cgcacggtta gcctccttga gccttatttg gtgccttcag
     4381 tcttgggttg tcgttcgatg atctggaact gcacgcgacg gttcttctga cgtccttcgt
     4441 ccgtgccgtt gtcggcgatg ggctgttctt ggccgtagcc cttgcccacg agacgcgcgc
     4501 ggtcgatgcc tcgcttgacg agagcagcaa cgacagcatc agcgcgacgc tgcgacaggc
     4561 ccttgttgta tccgggcgct ccggtgttat ccgtatgacc ttgaacctcg accttgagga
     4621 tctcgggatg gtctttgaag acggcagcca ctttgtcgag caactcatcg ctcaccgccc
     4681 ggatggtggc cttgttcgtg tcgaactgaa cctgttcgag gatgacgatt tccttatccg
     4741 ttacgtgaac cgtcgggcat ccgttcttct ttggatcggg atcccgcggc cccgcctcgg
     4801 tcggacaagc atcctcacta tcgatgatgc catcgccatc ggtgtcaggc ggacaaccgt
     4861 ttttctgcgg atcgtcgctc gccacaccag gcacatccgg acatgcatcc tgcgcatcga
     4921 aaatgccgtc cccatcgcga tcgggcggac aaccgttggt cttcggatca tccgtagcca
     4981 cgcccggaac atccggacaa gcatccaaag aatcgatgat gccatcgcca tctcgatcgg
     5041 gcggacaacc gttggtcttc ggatcatccg tagccacacc cggcacatca gggcaggcgt
     5101 cgttgatatc caaaataccg tctccatccc ggtccgccgg acatccatgc ttggccggat
     5161 cgtcgctcgg caccccagga acattggggc acgcatccaa atcgtcggtg ataccatcgc
     5221 cgtcggtatc gtaagggccg gccgcctttt cctgggaagg cgtgaaacca agcatgaaca
     5281 cccctcggaa cgaaggcgtt ccaagccctg tcgtcagccc gggccctacc ccgaggccgg
     5341 cttcaaagtg ttcgagaaaa cgccagcgac cgtccagaag tagctccacg ttcgagtttc
     5401 gggccgcatc gtcgtgcaac gctttgtcat ccgtggagag agtaaacgcc gcatatactt
     5461 ccggccccac ctggaactgt tttttgtcgc cgaacaagaa tcccacgcca gcgcccgcat
     5521 tgagttgcgt accatcgctc acgttggcaa agctctgccc cgcgcgaaac tgaggccccg
     5581 ccgccgcgga ccagaccacg ctctccgagc gcccccccac tacgagctgg ggcatcccac
     5641 gcactttgcc gttgccgaca tagccatcgg aaggtcccgt cggcaaccat gcataaccag
     5701 ccaatccgag ttggaaagca tcgtggtatt cgccgagaag gcgaagacgc aagccagcgc
     5761 gcacttcgcc catttgagcg ccgctggcgg aaggcaagga accggcctcg gaagggtttt
     5821 cgccatcttg gtaaagcgcg aagggcacat cgacattgag atggagacgt tcgaacaggg
     5881 gtaacgttcc gtttaaatgg gcaaatagct gcccagtaac gatattaccg gttttatcgt
     5941 ctgtctcggt attacgcaat acaagaggat tatgcgcata atcgagaatc agaccgacat
     6001 gtggtgtcaa atgccccgct acatagggcg actgaacgcc gaacatacgg tcgcccatgg
     6061 gcgctgggtc gaaccgattg agcggaagac catccgctcg tgcctcatgc ccagtggcga
     6121 accaactcgc tccgagcaca cccatcgtca cgatgctctt tagtcgcaaa gccatgttat
     6181 caagccactc cctgctttgg cgtgacgcca aagccgccaa ttgcccgctg cccaccttct
     6241 cgcccatcga gacggtggat acagcgagaa ttattatgca tacctctccc tatctgcaaa
     6301 cgatagcttc ggtcaatcga tgatctcgtc gatttatttt gtgtaaaatg taaaatatga
     6361 ataaaatggt gagccgattt cagtatcttc ggccaacatg cccaccgcgc gatgcttcca
     6421 tcctcctgtc cacgtcgatt gtcatgggtg agcctcctca cctagacacc gaaccgataa
     6481 cactccggaa atattacgta ttttctcgat cctctcctca ggacaaggct tgtcgaggtc
     6541 gatcaacgtc accgcaatct ccccccgaga acgattcaac atatcgagaa tattgagccc
     6601 aaccgccgcc aattccgtgg aaatctgacc caccatgttg ggcacgttgc tgttgacgat
     6661 cgccatccga tacccgctgt tgcgaggaag aaaggcattg gggaaattga ccgagttatt
     6721 cacggtaccg tgttccaaat actcccgaac ctgatcggca accatgatgg cgcagttgtc
     6781 ctccgcctcc ttggtcgacg ctccaagatg aggaaaagtc acgactttgg gatggttttt
     6841 tagcaggttg ctcgggaaat cacagacata ggcgtgcaaa tgcccttcgt tcaaggcggc
     6901 gaccaccgcc gtatcgtcga cgattcctgc acgcgaaaag ttaagcaaca ccgttcctgg
     6961 cctcatcgct ttgatgcgcg ttgcatcaat taggtgtcgc gtttcatcag aaaaaggaac
     7021 atgcaccgtc accatatccg aaagccccag caattcctcg atgctgccgg cggataaaac
     7081 gtccgtatca agctgccacg cccgctgcac cgtgatggta ggatcatatc ctaccgcacg
     7141 catgcccagc gccctcgcca cgttggccac cttcacccca atcgccccca atcccacgac
     7201 gcccaacgtg cgcccaggta attcaaatcc gctgaattgc tttttccccg cctccaccgc
     7261 tttcgaaatc tcggcatcac tgccctcgag cccccgcgca aatagccagc cggaaccaag
     7321 attgcgcgcc gccatcaaca aactcgctac cacaagctcc ttgaccgcat tggcattcgc
     7381 ccccggcgtg ttgaagaccg caatcccccg cgccgtcatc aacgacaccg ggatgttgtt
     7441 cgtcccagcc ccagctcgac ctatcgcttt gactgaatcc ggtatttcca gatcatgcat
     7501 tttcgccgat cgaaccagaa tcgcgtccgg tccgtccatt tgcgaagcca ccacataacg
     7561 gtccttcggt agcttctcca accctgcagc cgaaatattg ttcaatgtct gaatcttgta
     7621 catgccgctt tcccctaagc ctgtcgaatc gaccttgcaa gcccacatcg acgattcttc
     7681 cccgatattc cgtaagtggt tgaattaata taaagatatc cccatatccc tgccacgaga
     7741 cattctggca tatttgattt atctgtaaat agttaatggt tagaaaaaca gggaggcccc
     7801 cgccgacaaa tacggccacc ccaaccgaat cgtcatcccc acgctctggc tgaaatgcaa
     7861 tcgaaccccc gcatgaaaaa tgagcggctc caaatccaaa tccgcttccg tatccttgca
     7921 ccacccccct ggacacggaa cgtaatactc ccaactccga cgaacgatcg acggccccaa
     7981 ctccgccatg gcgctcacaa aatccgtgaa gaaaaagttc cactgcagca ccacgggaac
     8041 ccaaatttcg ttcgcggagc acttctcata ccaataatga taatagggat cgttggggga
     8101 cggacgtacc cgccatgcgg gactccaaca atgccccttg aagtgagccc aatccaaccc
     8161 aaaaccaatc gccagcgagt tgttgatcgt attgatcgga ccatcttcga taatcggaac
     8221 cgataaccgc acccccgggc cccacccatc atcatcacca taccaactat gcccgtcgtg
     8281 catgaatagc aaatgaggtt ccacctccac accgtatcga aggtgagtgc ctggttgttt
     8341 gatctgtgca tccgcaggat gaatcccccc cagccacgcc gtcatccccg cgcaccacgc
     8401 cgcaacccac aaagaccaac gaatacagca accatacctg ccagaatcac cggctcgctt
     8461 catctctcca ccatacgccg gggcgcttcc cactgtaaat ccccccgtga agcttgtgac
     8521 cccacgaacc tcgttttatc ccgtgtttgc acggaccatt tccaaacttt ctccatctca
     8581 gccccaactt gctcaacccc aactcgcccc gaaacccaca catggctctt ttgctcaacc
     8641 cgcaccacac aaccgcgccg ctctacccct cccgaaagaa ccaacaacac tgacttcctc
     8701 gattttcctt ctcgtccccc atcaatccga tgtcggcaac tcgacccatt gccccgctcg
     8761 aaccccgtgg cgccgcgtcc atcccgcatt gacctcgagc acgtaccgga caggacacgg
     8821 caccgaacgc gatgcttcgt tgagggtagg gacgttctcc acaatccccg caatgaaccc
     8881 atcctcctcg atgaacaaca tatcgagagg aatacaggtg ttgcgcatcc aaaagctttg
     8941 cacctccggt ctgccaggaa aatcaaatag catgcccgcg ttttccgcca tatgcgtccg
     9001 atacatcaac ccccgctgcc tttcccgcgg agaacgcgca agctccacct cgagcacagg
     9061 ggcgtcttgc gcctgcggga aagatacctg cgccttttcc aacgtcactc ccccaaacac
     9121 cgggtcgatc gggcaagact cggcgggacc aactggcggg ggcccttgtt gcggcgtggg
     9181 aatcatacag cggttcgccg attcacgacc cccagcctcg gacgatggcc catgattcga
     9241 acccacggtt cgcagcggtg ctcgtggccg atcgacaagc gccccctcgg gacgcctatc
     9301 acaaccggcc gcgcatacgc ccatcacgga caaaaaaccg atcatccaac gcatgggaag
     9361 gacatatcac gaactcaacc cgacacgcat gggctactcg cttacccaag gcaagaaatt
     9421 cacgtcgcgg attcggcacc ctcgaagcct cggtcagacc cgcacccgaa aatgctccca
     9481 agccgcaaaa cgcagttcat cgcgatggtc cggatgcgcg accgaaatca cggctcggat
     9541 gaaacccgat tggctattgc cggaaattcg cgccactcca tgctcggtca ccaagtaaaa
     9601 acgagcccct tccctccccc ccatcaaatc gggtcccacg tgactgatgg gtgttccttc
     9661 cggcatgaac ggcacgatcg aagaccgttg actaccatcg agcccacgat acgtggactt
     9721 caaacaaaga tagccgattc caccccgtga caatccccgc caaatgatcg caccgccacc
     9781 gattccggaa tggtgattcg aaaaacgccc cccggaattc acgtgaccgt gaaaatcaac
     9841 cgccagacaa ttgttgatcc caagcccgaa ataaaaccct tcgggaatga ccacccgatg
     9901 cgccggcccc
//
LOCUS       DAOSOR010000163         9895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6819_length_9895_cov_3.999390, whole genome shotgun sequence.
ACCESSION   DAOSOR010000163 DAOSOR010000000
VERSION     DAOSOR010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9895
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6819_length_9895_cov_3.999390"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            394..1671
                     /gene="hemL"
                     /locus_tag="PK710_10635"
     CDS             394..1671
                     /gene="hemL"
                     /locus_tag="PK710_10635"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOT10219.1"
                     /translation="MDTVSKELFERARMSIPGGVNSPVRAFRAVGGIPIFIQQAHGSR
                     ILGADGTEYIDYVCTWGPSIVGHAHPTVVRAVQEAATRGLSFGAPTKAEVVFAEQVRV
                     RYPSIDRVRCVSSGTEATMSALRAARGFTGRDLIIKFEGCYHGHVDSLLVKAGSGLTT
                     LATPDSAGVPEAMAATTLSLPYNDLESLEKVFAERGDAIAAVIVEPVAGNMGCIPPKE
                     GFLAAILSLCSKAGAVSIFDEVMTGCRLAPGGAQERYRLRPDMTCLGKVIGGGLPLAA
                     YGGKASIMSVIAPEGKVYQAGTLSGNPVALAAGLATLELMDDLAYDRLEYLGDLLERG
                     LRSALADRGIPGCVQRVGSMLTLFFHPGPVERWDQAVQADTKRFAKWHAGMLSRGVYW
                     PPSQFEAAFLSVAHSEADIESTIKAARDAMEKL"
     gene            1750..1905
                     /gene="rpmG"
                     /locus_tag="PK710_10640"
     CDS             1750..1905
                     /gene="rpmG"
                     /locus_tag="PK710_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010364212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOT10220.1"
                     /translation="MRELIRLVSSAGTGYFYVTTKNKRTMAQKMKIKKYDPRVRKHVE
                     FTEAKMK"
     gene            2046..2957
                     /locus_tag="PK710_10645"
     CDS             2046..2957
                     /locus_tag="PK710_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT10221.1"
                     /translation="MDRSTVLIRPEDARKQPGIWLGAIRERASAVLQHVTGTDFEEKI
                     RRLEAYRGHERMDSFGLDLEWVRVALVAVAVLHRFYFRVDASGLENVPAGRVLLIANH
                     SGQLPIDAALVAASMFFDAEPPRVVRSMVDKWTQTLPGVCTLFARCGQVVGVPENAKK
                     LLDSEEALLVFPEGTTGISKPFSERYRLKDFGLGFMRLALETRTPIVPIAVLGAEEQY
                     INVGNLETLAKALRMPVFPIIPQWLLPGGLLPLPTKYRIRFGQPLMMTGDPDDEDAVI
                     EPKVEIVRSAIQSMLNRGIKERQHVFW"
     gene            2979..3953
                     /locus_tag="PK710_10650"
     CDS             2979..3953
                     /locus_tag="PK710_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOT10222.1"
                     /translation="MRKNSNATKTSRKETAAAPPVVLITGICGRLGQRLTRLLHRQQH
                     VIGIDRRPFLSFPKDVEHFAVDLRRKKMRDIFRGRDIDAVVHMGIMHNPRTSSAEHHT
                     WNVLGFQRLLENVAQFHVRKLIVLSGAAVYGARPDNPQFLEEDAPLLGGANWSGLRDL
                     IAVDMLAQSFFWKSPSTETVIFRPVHILGSVRNGSSNYLRLPRVPTLLGFDPMMQVIH
                     QDDVVEAIRMALRPGIRGIYNLAGPPPIALSHLIQIAGKPRFAIPHPMAEIAVRKLWK
                     IGMTSFPAPELDHIRYVCMVDDERARSVLGFQHRHDVNAAVRAVFETE"
     gene            3967..4221
                     /locus_tag="PK710_10655"
     CDS             3967..4221
                     /locus_tag="PK710_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10223.1"
                     /translation="MVGLMGVVTLSGELGCSSNSGEQPTAQRALPSGIPPAAARALGP
                     GAATISSTMDNVLPESPIDPRAPVDPDAGLAPPAKEPLPL"
     gene            4232..4462
                     /locus_tag="PK710_10660"
     CDS             4232..4462
                     /locus_tag="PK710_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10224.1"
                     /translation="MMTFSHLIVRAGMMALALGCSTAVPPPNLPAPEFEPPRPIEEPK
                     PEPGVDDAALDAPDVPLPQTTQPQTEQEPSPP"
     gene            4614..6878
                     /locus_tag="PK710_10665"
     CDS             4614..6878
                     /locus_tag="PK710_10665"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT10225.1"
                     /translation="MFRKNASDAFGFLASCGWVMVVWDTVALVQARQANLTWDFFSVL
                     MASAALTMAHVLVVGAVVAPMYAFALGDQRIATRLKNAIIAVSAALKTRSAPSAVSVH
                     ACLLAAPAALLGYVFVAVPLAMRVHEDVRVAINAAGASIVIHLLAVFAGALLFAVLRG
                     VWMSLLRPLASRRLTQIWLMRPWLTAAFLSLFALFAMVWAVVMGRRVLSALDLRMLWI
                     GLTVMVLGAILATVRTIPRLSKTTRRVIGWSAIIGGVIFTLAATFAVPYSSHARSMLV
                     AHTTFGTVFHASHNTLFDWDGDGYTTLFGGNDCAPFDPTIHPGAVDIPYNGIDEDCSG
                     EDFVFDVAPPLRRWDYPVPDRSKKPHIVLLTIDALNPLHLGFNGYDRPTSPHLDAFAK
                     HAVRFENAFAQGPSTRLSIPSLFTSLYDPQIKRAKLGRIPLAVLSENVTVAEILQKEG
                     YRTIAVVPSPYFTGWGGITQGFQEVDGSAAKLKQAHTSKEVSDAVIERLESVSEQGQP
                     VFLWAHYFDPHGPFDQPPDAPVFGTKAMDVYDAEIRFTDEHLGRVLSWIEEHWAEQDR
                     LLVVTADHGESFDDQHAVKHHGYDLNTSVLRIPMLIHMPIFAPHSVRTPVSLLDMAPT
                     FINLAQAKGEFDFQGSSLVPMLQGEEMAERVTFHTFFLPEFVKKQKDPLRMVSVRTDR
                     FNLVHDREKGVYSLFDFVQDQNETNNLFDADRATADHLMAQMQYWYYRVLDQGGSKEN
                     AGAATATPRPPIVR"
     gene            complement(6844..8835)
                     /locus_tag="PK710_10670"
     CDS             complement(6844..8835)
                     /locus_tag="PK710_10670"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOT10226.1"
                     /translation="MNRKYGWLSHFRRLGYLLLSGLIACGGNNSTSPSGSGQEDGADD
                     TFWVDGKADVGGIAEGSAEACGVLAVANDSTFEQLKTDVGLSVKAAKNIVAYRQGSDG
                     TLGTGDDGYFDSLDELDKVKYVGPVAFSKLATYAKGHDKACKIVDLQFISLSDFHGQL
                     DPITVAEVGKVGGAAALNYYFVKDRVANPRSLLLSAGDSFGASPPIAAFFDERPVIEA
                     MNRMRFDGEAPGNHSFDRGVPALQELIELADFPFVSANLTGVEDSMACETKPDKLCIA
                     PYQNYWVGGVKVAVIGLLTPEAPSLVKPGSFASIEVTDPAASAQAAKAKAAAEGASVF
                     VALTHIGGSPGAGGAAPTGPLMELAEQLDGFDLLIGGHTHNAIETTVGNLLVVENPSQ
                     GAKYSRVALSFDFAQRKVVHKTSEMLTPVVEGTVPDPAFDEFMAHYRTQLAQQLDLPL
                     AVAEGIFERGNNVERLQEVALGNLLTDALRARYQTDLALVNGGGLRAPMPSSYAPANL
                     SLRRPSPDYQAGPPFDLVVGDVFAVLPFVNDAAICTLTGKQVWAMLEHGLGFLPAPNG
                     GFPQVSGITVTFDSAAPAGNRVISIVLDNKTAIDKNDTTYSLVTSDFMYLGGDGYSML
                     SQADGYVADRLTDVLADYIEAVGTIAPTINGRLVDVASP"
     gene            complement(9049..>9895)
                     /locus_tag="PK710_10675"
     CDS             complement(9049..>9895)
                     /locus_tag="PK710_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOT10227.1"
                     /translation="DGIPDDRDQCRDEPEDFDGFQDEDGCPDPDNDGDGIPDSLDQCP
                     NEPEDFDGFQDEDGCPDLDNDGDGIPDSKDLCPNMAEDFDGYQDHDGCPDPDNDGDGI
                     PDEKDACPMSPEDVDGFEDEDGCPDLDNDGDGIPDSLDQCPNEPETINGIDDQDGCPE
                     PEAKDQTVLQGMTVAVIDPQRFAPGSSRMTAALRKQLLMMAQRALGVSPVQMIVVQTY
                     ADSPGTTERNENLAADRADAIRKELLEAGLPADKLTVAVGELTLARPVRASQFDMTVQ
                     RGKHD"
BASE COUNT         2082 a         2766 c         2799 g         2248 t
ORIGIN      
        1 tatctgggcc tgcttccgcc gaaggggaaa gtcctcccgc agtttgtacc aaaggcggat
       61 cgcaagccaa gccaagggcg ccgatggtaa cgagtcgcgt gagagcgagc gggtagggca
      121 tggcggcaag catagcatgg gaggcaaaag caaggattgt gtccaagctt ggcgatgatt
      181 tggggtgaac aggggcatga ggaatgggaa ttggcgaatc ccatgagcgg catttttcgg
      241 atgggacaag gaacgcgaga aggccgatgc caatcacgga tgatgtcggt cgtcgaaacg
      301 agccaagcag ccagttcgaa gaggttcatt tcagcctcca aagatccgct aaggtatcga
      361 cctagcgaga caatcaggct atacccggcc cccatggaca ctgtatccaa agaactcttc
      421 gaacgcgccc ggatgtccat cccgggcggt gtcaacagtc ccgttcgcgc attccgcgcg
      481 gtgggtggta ttccgatttt cattcagcaa gcgcacggtt cacgcatcct tggagcagat
      541 ggtaccgagt acatcgatta cgtctgtacg tggggtcctt cgattgtcgg gcatgcgcat
      601 ccgaccgtgg tgcgggcggt gcaagaagcc gcaacccgag gtctgagttt tggggcacct
      661 accaaagcgg aggtggtgtt tgccgaacag gtgcgtgtac gctatccgag catcgaccgc
      721 gtgcgttgcg tgagcagcgg aacggaagca acgatgtcgg cgttgcgtgc ggcccgtgga
      781 tttactgggc gcgacctcat catcaagttc gagggttgct atcacggtca cgtggattcc
      841 ttgctcgtca aagcgggaag cggcctaacc acgctggcga ctccagactc ggcgggcgta
      901 cctgaagcca tggctgcaac cactctatcc cttccctaca atgacctgga aagccttgaa
      961 aaagtgtttg ccgagcgagg cgatgccatt gccgcggtga tcgtggaacc cgtggccgga
     1021 aatatgggtt gtattcctcc gaaagaaggc ttcctcgcgg caatcctatc gctttgttcg
     1081 aaagcgggcg ccgtatccat tttcgatgaa gtgatgaccg gatgccgtct tgctcccggg
     1141 ggagcgcagg aacgatatcg tttgcggccc gacatgacgt gcctgggaaa agtgatcgga
     1201 ggcggtctgc ctcttgctgc ctatggcggg aaagcgagca tcatgtccgt gatagctccg
     1261 gaaggaaaag tctaccaagc cggtacactc agcgggaatc ccgtggccct tgcggcaggt
     1321 ctcgccaccc tcgaactcat ggatgacttg gcctatgacc gtctcgaata tttgggagat
     1381 ctgttggaac gagggttacg gagcgctctg gccgaccgag gtatcccggg ctgcgtgcag
     1441 cgtgtgggtt ccatgctgac tctatttttc catcctggac cggtggagcg ctgggaccag
     1501 gccgtgcaag cagataccaa acgcttcgca aaatggcatg ccgggatgct ttctcggggt
     1561 gtgtactggc ctccctccca attcgaagcc gccttcttgt ccgtcgccca tagcgaagcg
     1621 gatatcgaat ccaccatcaa agctgccaga gatgctatgg aaaagctctg agggaaaggg
     1681 ttgatcacga tccttcttgc cagatacaac gactaggctt atacagccgc ggcttttacg
     1741 aggaccccca tgcgagaact cattcgatta gtctcttccg ccggaaccgg ttacttctac
     1801 gtgaccacga aaaacaagcg tacgatggcg cagaagatga agatcaagaa atatgacccg
     1861 cgtgtgcgaa aacacgtaga gttcaccgaa gcaaagatga agtaaacacg atcgtctttg
     1921 catttccttt ccctggcttg tgccttggta gcgcaagcct tcttcgttaa tccgcgcaat
     1981 cgatttgcat cttcttttcc gcaaacactt tcagtgcttc gcggagcggg gtacgctacg
     2041 gcccgatgga ccgctctacc gttctcatcc ggcccgaaga cgcgcggaag caaccgggta
     2101 tttggttggg agccattcgt gaacgagcat ctgctgtctt gcagcatgtg acgggaacgg
     2161 acttcgagga aaaaatacgc cggctcgagg cctatcgcgg tcacgaacgc atggactcgt
     2221 tcggcttgga cttggaatgg gttcgtgtgg cgctggtggc cgtggccgtt ttgcaccgct
     2281 tctattttcg ggtggatgca tccggtttgg agaacgtgcc agcggggcgc gtccttctta
     2341 tcgcgaacca ttcggggcag cttcccatcg atgcggcttt ggtcgcagct tcgatgtttt
     2401 tcgatgccga gccccctcgg gtggtgcgga gcatggttga taagtggacc cagacgcttc
     2461 ctggggtttg cacactattt gctcgctgtg ggcaagtggt gggcgtaccc gaaaacgcga
     2521 aaaagcttct tgattccgag gaggcgctat tggtcttccc ggaaggaact accggtattt
     2581 caaaaccttt ttctgaacgc tatcgcctca aggacttcgg tcttggtttc atgcggctgg
     2641 cgttggaaac ccgaacgccc atcgtcccca tcgcggtgct tggtgccgaa gagcaatata
     2701 tcaacgtagg aaacctcgaa acgcttgcca aagcgttgcg aatgcctgtc tttcccatca
     2761 tccctcaatg gctgctgccg ggagggttgc tgcccttgcc cacaaaatat cgcatccgtt
     2821 ttgggcaacc tttgatgatg acgggcgatc ctgacgatga agatgccgtt atcgaaccca
     2881 aggtggaaat cgttcgaagc gcgatccaat cgatgctcaa tcgaggcatc aaagagcgac
     2941 agcacgtttt ttggtaggcg cggggaggct ttcgagtggt gcgtaagaat agcaatgcga
     3001 cgaaaaccag ccgaaaggaa accgctgcag caccaccggt cgtcttgatt accgggattt
     3061 gtggaagact cgggcagcga ctcactcgcc tgttgcatcg ccaacagcat gtcatcggca
     3121 tagatcgtcg gccgttccta tccttcccga aagatgtaga gcacttcgcc gtggacctgc
     3181 gtcgaaaaaa aatgcgggac atctttcgcg gccgcgacat cgatgccgtc gtgcatatgg
     3241 gcatcatgca caatccaagg acgagttcgg cggagcatca cacgtggaat gtgcttggtt
     3301 tccaaaggct tttggaaaac gtggcgcagt ttcacgtgcg aaaactcatc gtgctttcag
     3361 gagccgccgt gtatggcgca agacccgata atccccagtt tctcgaagaa gatgcgccct
     3421 tgttgggagg cgcgaattgg agcgggcttc gcgatctcat cgcggtggat atgctggctc
     3481 aatccttttt ttggaaaagc ccatcgaccg aaaccgtcat ctttcgtccg gttcatatcc
     3541 tgggctccgt gcgcaacggc tcttccaact acttgaggtt gcctcgtgtg cccactcttc
     3601 ttggcttcga cccgatgatg caagtgattc atcaagacga tgtggtcgag gccatccgta
     3661 tggcgctgcg accggggatt cgaggcattt acaatcttgc ggggcctccg cccatcgccc
     3721 tatcccatct catccagatc gctggaaagc ctcgctttgc cattcctcat cccatggcgg
     3781 agatcgccgt tagaaagctg tggaaaatcg gcatgacgtc gtttcccgca cccgagttag
     3841 accatattcg ctatgtatgc atggtcgatg atgaacgcgc tcggtcagtt cttgggttcc
     3901 agcatcgcca tgatgtcaat gccgcggttc gtgcggtttt tgaaacggaa taaccagttt
     3961 ttcatcatgg ttgggctgat gggggtcgta acgctgtcgg gagaactcgg ctgttccagc
     4021 aactcgggtg agcagcccac ggcacaacga gcgctccctt ccggaatccc accggctgct
     4081 gcgcgtgcgc tcgggccggg agcggccacc atctcctcga cgatggacaa tgtcttgcca
     4141 gaaagcccca tcgatccaag ggcacccgtg gaccccgatg ccggcctggc tcctcctgcc
     4201 aaagagccct tacccctttg atcggatcgt catgatgacc ttttcgcact tgatagtacg
     4261 cgccggaatg atggcgctcg ccctgggctg ttctacggcg gtgcccccgc cgaacttacc
     4321 cgcgccggaa ttcgaacccc cgcggcccat cgaagagcca aagcccgagc caggtgtcga
     4381 cgatgcggca ctggacgctc ccgatgtgcc actgccgcaa accacacaac cgcagaccga
     4441 acaagagccc agcccgccat gatccatggc gcgcgcagcg aggattgatt cgcgcttcgt
     4501 tccatggatc acggaacgaa gcagaaccta ccccgatgtg agtttacgca agaaacgagg
     4561 atgctacggt ctgtcgttcg tttggttttg gcccttgaaa taggagaaaa aggttgtttc
     4621 gtaagaacgc atcggatgct tttggttttt tggcatcgtg cggctgggtc atggtcgtgt
     4681 gggatacggt tgcgttggtg caagcacgac aagcaaacct aacctgggat tttttctccg
     4741 tgctgatggc cagcgcggct ttgacgatgg cgcatgtttt ggtcgtgggg gctgtagtcg
     4801 ctccaatgta tgcctttgcg cttggggatc agcgcatcgc aacgcggctg aaaaatgcga
     4861 ttatcgctgt gtccgcagcc ttgaaaacac gttcggcccc ttcagcggtt tctgtccacg
     4921 cttgcttgtt ggccgccccc gccgcgctcc ttggctatgt gttcgtggcc gttcccctgg
     4981 ccatgcgcgt tcatgaggat gttcgtgtgg ccatcaatgc cgccggtgcc tccatcgtga
     5041 tccatcttct tgcagtattc gccggagcac ttttgttcgc ggtgctccgc ggtgtctgga
     5101 tgagcctgtt gcgccccctg gcgtcccgtc gtctgacgca aatctggctg atgcgcccct
     5161 ggcttaccgc cgcttttctt agcttgttcg cgctattcgc catggtatgg gccgtggtga
     5221 tgggaaggcg tgttctaagc gcgctggact tgcggatgct ttggataggt ctcaccgtga
     5281 tggtcttggg agccattctc gccacggtga gaacgatccc acgactgtcg aaaacgacaa
     5341 ggcgtgtgat cggctggtct gccatcatcg gaggagtcat ttttacgttg gcggctacct
     5401 ttgcggttcc gtattcgtcc cacgcccgtt ccatgctcgt ggctcacacc acctttggca
     5461 ccgtgtttca tgccagtcat aacacccttt tcgactggga tggcgatggg tacacaacgt
     5521 tatttggggg taacgattgc gctccgttcg atcccacgat tcatccgggg gccgtggata
     5581 ttccctacaa tggtatcgat gaggattgct ctggtgaaga tttcgtattc gatgtggctc
     5641 ctcctttgcg acgttgggac tatccggttc ctgataggtc gaaaaaacct catattgttc
     5701 tgttgacgat cgacgcgttg aatccgctgc accttgggtt caatggatac gatcgtccca
     5761 catcgcctca tctcgacgct tttgcgaagc atgcggtccg tttcgaaaac gcatttgcac
     5821 agggacctag cacccgccta tccatccctt cgctgtttac ttcgctttat gatccgcaaa
     5881 tcaaaagggc caaactcgga cgaattcccc tggctgtact gtcggagaac gtgacggttg
     5941 ccgaaatcct acagaaggag ggatacagga ccatcgcggt ggtaccatcg ccgtacttca
     6001 cgggctgggg cggcattacc cagggattcc aagaggttga tggctccgcc gccaagctca
     6061 agcaagcgca tacctcgaaa gaagtctccg atgccgtgat tgaacggctg gagtccgtat
     6121 ccgaacaagg tcaacccgta tttttatggg ctcattactt cgacccccac ggacctttcg
     6181 atcagccacc tgatgcgccg gtgtttggaa cgaaggccat ggatgtgtac gatgccgaga
     6241 ttcggtttac cgatgagcat ttgggccgtg tgctgtcgtg gattgaagag cattgggcgg
     6301 agcaggatcg tctgctggtg gtgacagccg atcatggcga aagctttgat gatcagcatg
     6361 cagtcaaaca tcacggttat gatctcaaca cgagtgtgct tcgcattccg atgttgatcc
     6421 acatgcccat cttcgctccc cattctgttc gcacccccgt ttcgttgctg gacatggcgc
     6481 cgacgttcat caacctggcc caggcgaagg gcgagttcga cttccaaggc tccagtctcg
     6541 tgccgatgct gcaaggggaa gagatggccg aacgtgtgac gtttcacacg tttttcctcc
     6601 cggagttcgt caaaaaacaa aaagacccgc tgcgcatggt gtcggttcga accgaccgtt
     6661 tcaacttggt ccacgatcgc gaaaaaggtg tctattcgct tttcgatttc gtgcaggacc
     6721 agaatgaaac gaacaatcta ttcgatgcgg atcgcgcgac ggccgatcat ctcatggcac
     6781 agatgcagta ttggtattat cgggtattgg atcagggggg aagcaaagaa aatgctggtg
     6841 cggctacggc gacgccacgt ccaccaatcg tccgttgatt gtcggtgcaa tcgttcccac
     6901 cgcttcgatg taatcagcca ggacatcggt gaggcggtcg gccacatacc cgtcggcttg
     6961 cgaaagcatc gaatagccgt cgccgccgag gtacatgaaa tcactggtca cgagcgaata
     7021 ggtggtgtcg ttcttgtcga tggcagtttt gttatcaagg acgatggaga tgacgcgatt
     7081 gccggctgga gcggcagaat cgaatgtgac cgtgatgcca gagacctggg gaaatccgcc
     7141 attgggagct ggcaaaaacc cgagaccatg ctcgagcatg gcccacactt gcttgcccgt
     7201 cagcgtgcaa atggccgcat cgttgacaaa aggcagcacg gcaaaaacgt cgccaaccac
     7261 caggtcgaaa ggaggacccg cttggtagtc cggtgagggg cgacgcaacg acaagtttgc
     7321 cggggcgtag ctcgaaggca tgggagcccg caatccaccg ccattgacca gagccagatc
     7381 ggtttggtag cgagcgcgca gggcatccgt gagtaggttt ccaagggcta cttcttgaag
     7441 cctttccaca ttgtttcccc gttcgaatat cccttccgcc acggccaggg gaagatccag
     7501 ttgttgggcg agctgggtgc gataatgagc catgaactca tcgaatgccg ggtccgggac
     7561 ggtcccttcc acgacgggtg tgagcatttc cgatgtctta tgaacgactt ttcgctgcgc
     7621 gaaatcgaac gataaagcta cacggctgta cttcgctcct tggctgggat tttcgacaac
     7681 cagaaggttg ccgacggtcg tttcgattgc gttatgcgta tgcccgccaa tcaataggtc
     7741 aaaaccatcg agctgttcgg ccaactccat caaaggaccg gtgggagcgg cacctcccgc
     7801 gccaggggaa ccgccaatat gcgtcagagc tacaaagaca gaggctcctt ccgcagcagc
     7861 tttggcttta gccgcttgtg cggaagccgc tggatcggtc acctcgatcg aagcaaagct
     7921 tccaggtttg acgagcgaag gggcttcagg ggtcagaagc ccgatgaccg ccaccttgac
     7981 accgccgacc caataattct gatacggcgc gatgcagagt ttatcgggct tcgtttcaca
     8041 tgccatggag tcctcgacac cagtgaggtt ggcggagaca aacggaaagt ccgccaactc
     8101 gatcagctct tgcagcgccg gaacacctcg gtcgaagctg tggttgccag gagcttcccc
     8161 atcgaagcgc atccgattca tcgcctcaat cactggcctt tcatcgaaaa atgcagcgat
     8221 gggaggactc gccccaaaag aatctcccgc ggacaaaagc aggctgcgcg ggttggcgac
     8281 acgatctttc acaaaataat aattcaacgc cgctgctccg cccaccttgc caacctcggc
     8341 caccgtgatc ggatcgagct gtccatggaa atcagatagc gaaataaact gcaagtccac
     8401 gattttacag gccttatcgt gacctttcgc atacgttgca agcttcgaga aagccaccgg
     8461 ccccacgtac ttcaccttgt cgagttcatc caaagaatcg aaatacccat cgtcgccagt
     8521 ccctagcgtg ccgtccgatc cttgccggta agcgacgatg tttttcgcgg ctttgacaga
     8581 aagacctacg tccgttttga gttgttcgaa cgtgctatcg ttggccaccg ccagcacgcc
     8641 acaagcctcg gcgctcccct cggcaattcc tcccacgtcg gctttgccgt ccacccaaaa
     8701 cgtgtcgtcc gcgccatcct cttggcccga cccggaaggc gacgtcgaat tgttgccccc
     8761 acacgcgatg agcccggaaa gaagcaagta accaagccga cgaaaatgcg acaaccagcc
     8821 atatttccta ttcatttcga cgacatgccc tttctttccg caacgattac cgtacaggtc
     8881 aatggcggga tatgtagttg aataccgtac ctgtctgcta ggagcagata gttgaagtgg
     8941 gatggttatg caagctttga ggaggggtgt ttgggaaaag ccggaaacgg cgggggtatg
     9001 tatgaacgtg caacttcgcc aacctcagat ggcgcggcca acgagcagtc agtcgtgttt
     9061 cccccgctga accgtcatgt cgaactgcga agcgcgcacg ggacgcgcca gggtcaattc
     9121 tcccacggcg accgtgagtt tgtcggctgg caatcccgct tcgaggagtt ctttgcgaat
     9181 ggcatctgct cgatcggcgg caagattttc gtttcgttcg gtggttccag gcgaatcggc
     9241 ataggtttgc accacgatca tttggacggg agatacaccc agggctcgct gggccatcat
     9301 cagaagttgc tttcgtagcg cggcggtcat tcgcgatgag cctggcgcaa agcgctgtgg
     9361 atcgatcacc gctaccgtca tgccttgtaa cacggtttga tctttggcct ccggttcggg
     9421 acagccatcc tgatcatcga tgccattgat ggtttccggc tcgttcggac attgatccag
     9481 cgaatcgggg ataccatcac catcgttatc aagatccgga catccgtcct catcctcgaa
     9541 gccatcgacg tcctcgggcg acatgggaca ggcgtccttt tcgtccggaa tcccatctcc
     9601 atcattatcc ggatcgggac atccgtcatg atcctggtag ccatcgaaat cttcagccat
     9661 gttgggacat agatccttcg aatccgggat accatcgcca tcgttatcga gatcagggca
     9721 cccatcttca tcctgaaaac cgtcgaaatc ctccggttca ttcggacatt gatccagcga
     9781 atcagggata ccatccccgt cattatccgg atcaggacat ccgtcctcgt cctgaaaacc
     9841 gtcgaaatcc tctggctcat ctcggcattg gtctcggtcg tccgggatac catcc
//
LOCUS       DAOSOR010000164         9853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6861_length_9853_cov_4.905899, whole genome shotgun sequence.
ACCESSION   DAOSOR010000164 DAOSOR010000000
VERSION     DAOSOR010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9853
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6861_length_9853_cov_4.905899"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="PK710_10680"
     CDS             complement(<1..1170)
                     /locus_tag="PK710_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10228.1"
                     /translation="MKKTYILRSMMGIGAWLVFSACDQPKTTSSTVSSSPSPSVSAPK
                     VVVYNAIDRGTFNRVAVQMDIPIFWIEDKNGNRAIDPNEVASLQFYPTQGNWVDGGRF
                     TARFEEVYRQMQQWAKAPAFPKDLSDAEKTRRQLIIEELDQGQPTLVFSDFTKNSPQD
                     KVLIRHMLAAAKLIDDLHGMQRGLAPLETQIPKDDPSSHAAFRRNWGPKCGAPKTEKN
                     PACSAIPKLPDKIPVAMYPQEMQSEEGFCEKLAKQADGEKLMSPFVVVKKEGDKLVTE
                     PLTEAFQDLSEKVSKELRLASDGLGAEEAPFKEYLQAAAQAFLDNRWEPADEAWSKLN
                     ATNSKWYLRIGPDEVYWEPCSRKAGFHMSFARINEASVAWQKKLEPVQQEMEDELA"
     gene            1283..1960
                     /locus_tag="PK710_10685"
     CDS             1283..1960
                     /locus_tag="PK710_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase A"
                     /protein_id="HOT10229.1"
                     /translation="MPAPAFAPFAAAHITAPGSSLPLPALGHALPDGGLPRGAVVELS
                     SHGSSLASSLALYACACAQQQTWLKSGQPAWCAWIDPGQSLYAPGVAAHGVVLERLLV
                     AYPLPHTLPQTAVRMTLSQAFATLIIDTQGVVGFPLPLSLDRWPNIVRRLSLAARDGS
                     TCVILLTQRSRSQHLGLPVALRLELKQPKPGVLHVEVAKERQGRMGACAELAWTHLSN
                     PWCSQSA"
     gene            complement(1967..2971)
                     /locus_tag="PK710_10690"
     CDS             complement(1967..2971)
                     /locus_tag="PK710_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT10230.1"
                     /translation="MGRKQKSDLDPHKVIHVDFGMSRRLPHDLPQTPDQDLPPLPSAR
                     EPITDLFSGTEVCRLLGLSRGQLRSLDRTGVVSPSGRRRGRRAYTFSDLIALRTASHL
                     LEHNVRLRDVARAVTKLKHTLPKVIRPLSELRVVSDGTKVIVRGQDGAYEPSTGQMLL
                     DFEVETLRNDVVRVLRPTVSRQRTQSAYELYERASRLDESPATYDEAEELYRQALELD
                     PYLAIAYTNWGNICFRRCNDKAAERLYRRALEIDGKQPEAHYNLGYIMLERGEPGEAI
                     TYFQAAIASDPTFADAYFNLAMAFEHLGDEQQSRPHWQKYVQLEPHGTWAEIARKHLL
                     "
     gene            complement(3129..3566)
                     /locus_tag="PK710_10695"
     CDS             complement(3129..3566)
                     /locus_tag="PK710_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10231.1"
                     /translation="MNPWQGPGIRKVRHPHPETVRVLSFVQVGDAFWSSLLEDADVIS
                     EGATSPEGSSLVYRGSTSIMVRSDTMALVTHGSKGRAMIIDPHLRLRAMRIARREAQA
                     RAPGMLGPLQMDLFVHAEARALRIDIDVDAVVEHRASRMGREA"
     gene            3689..4057
                     /locus_tag="PK710_10700"
     CDS             3689..4057
                     /locus_tag="PK710_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10232.1"
                     /translation="MAKQPENYRGLDRRRHRILITENTEYHFRDGLCIAVRDRQTGEW
                     MTDHVALLRPVSGSLQILPNGAIRPNIGPPRIGEALFFATGGRDLITSPLLQEKRPSK
                     HVVDAYPAGLRGLRRRFSHH"
     gene            4127..4672
                     /locus_tag="PK710_10705"
     CDS             4127..4672
                     /locus_tag="PK710_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10233.1"
                     /translation="MACTSQRLLELLNEAGFRYWTAPNPEDGCLVSFMGNHATRPTVF
                     VHVRVDSDGSVLTAMVTAFLRVADHPSLPVLMQAMLHEAYLTVLGQWEMDPSDGEVRV
                     TVQLPLFDAEPTANQVARTVTAAARLADRAWPRLLAILETGQDPVLEEDDDDPARKEL
                     RRLQIRMQELLQAQRSKTDQN"
     gene            4682..5221
                     /locus_tag="PK710_10710"
     CDS             4682..5221
                     /locus_tag="PK710_10710"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HOT10234.1"
                     /translation="MGTTIQEITAIFKEANIHFDVDEDLDLIRSLWSRGESTVTLVVF
                     VLEDGELVQLRAPTLLQVDDDANKPLLFRAMLQMAYEIRLVQFEYDPADGEVSTCIDI
                     ALEDGKLTSAQLLRCCAVLLDVAFLAHKRLTTILETGKDPALDSEDVDEEEAEQRQSQ
                     LDAMAKLAALIRRGGGGEA"
     gene            complement(5225..6463)
                     /locus_tag="PK710_10715"
     CDS             complement(5225..6463)
                     /locus_tag="PK710_10715"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOT10235.1"
                     /translation="MGIIGVLFFVAIVVAAFILITIKRMLYVVPPNQAMILSGAKRKI
                     GNKVLGFRVVCGGRALRIPLLERVDFIDLRNINIDVEVRGAFSKGGIPLNVKAVANVK
                     LPGEEPMIHNAVERFLGRNRNEIMYIAKETLEGNLRGVLAQLTPEEVNEDKNRFTHIL
                     TEEAEHDLSKLGIKLDQLKIQNVTDDVGYLNSIGRIRGASLRQRAVIAETQAQSGAAE
                     QKALNWNASEIARYDAEIAIAEREMAKRIVDARTRRDAMIATAEGEVRAQIAKVMAEI
                     KRQQARAVQVERQLEADVVQPADAARLQMEEAAVGAAAAIVERGKAEAAALHQLVEAY
                     KKGGAAAKEVLVLQNILPMIQKISGAEHPVRIAKVTVLPRASGEQEPLARKIIGANEQ
                     LKAATGVDLASVAARITSKS"
     gene            complement(6463..7755)
                     /locus_tag="PK710_10720"
     CDS             complement(6463..7755)
                     /locus_tag="PK710_10720"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOT10236.1"
                     /translation="MMESSGVEGAALSAIVLGASVFTLILLGAMLLIIQRLLLICPPN
                     KVLIFSGRKHKLPDGSVVGYRVLHGGRGIRIPLLETVSELDMRIFPVQVTVQNAFSKG
                     GIPLAVQAIANVKISSDPRFLRSAAERFLDVPVQRLAEVAKDTLEGALREVLAELTPE
                     EVNQDRLKFADKLLSNAGDDFERLGLQLDVLKIQHVSDEQGYLTNLGREAIANMLRDA
                     ENAENAANQTIAEAQAAARTRAETALKNAEAQVLKKKNEYQTQLAKLEAEAASAENEA
                     TVDAETARSMAEQNLQEMRKQYEQLRLACDVVLPAQAKRRAEELLARGEASPFIETGK
                     ARAIALQAVVAEWTAAGLQGREIYVLNHLKELVEAAVQRTASTQIKEINVVDGGDAAS
                     YGSFVAAFPQVVSQIMQETGQAVGVDLGAMLQSRKAGG"
     gene            complement(7868..8449)
                     /locus_tag="PK710_10725"
     CDS             complement(7868..8449)
                     /locus_tag="PK710_10725"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOT10237.1"
                     /translation="MGIVYLAALILGLGTLTLQFILSHAGGADADAGGGDGADGTHEL
                     DDAHGGGAYGAAGVFLSMRFWTFALMAFGMVGSLLHYFDMAGFGLSLGLSVASGVLSG
                     LAASLVFRALRGSVSSTMGEQDTIGKMAKVTIPVKKGSLGKVRVMVKGKQVDMLAITD
                     ADSIETGDEVVVVEYRGEYACVEPIGKPSEPSE"
     gene            8602..>9853
                     /locus_tag="PK710_10730"
     CDS             8602..>9853
                     /locus_tag="PK710_10730"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOT10238.1"
                     /translation="MDPSRVEKALRLLHRSLGPSKIITSKDGCEPFARDESEAVGRTP
                     DAVVLASTPKDVVLTLDCARRAGVPVTPRCAGTGRTGGAVPLRGGIVLSTLGMGDIKE
                     INHHDRVAVVQPGVILEQFHDAVEREGLFYPPDPNSRASCGLGGNIAENAGGPRAVKY
                     GVTRDYVLGVEMAAMTGPLIRMGRRTVKGVVGYDMTGLFVGSEGTLGVVTEATLALLH
                     KPDMVLTLLAIFPTPHEAGRAVAAIFRSRICPRCVELMDAGALQAVRAQGVGIDPLAG
                     ALLLIEVDGHPAVVAEEAEGVRKILAEAPADEVHEAKNAQEAAGLAAARRTALSAVAR
                     VSPTTILEDTTVPRSHLAETFAEIERLKAKYQVRVATFGHAGDGNLHPTAVTDERNRK
                     EIENVHAFFAELYEKVLAIGGTVSG"
BASE COUNT         2026 a         3099 c         2596 g         2132 t
ORIGIN      
        1 cgcaagctcg tcttccatct cctgctgtac gggttcgagc ttcttttgcc acgccaccga
       61 agcctcgttg atacgagcaa aactcatgtg gaatcccgcc ttgcgactgc acggctccca
      121 atacacctcg tcgggcccga tccgcagata ccacttggaa ttcgtcgcgt tgagcttgga
      181 ccaggcctcg tccgcgggct cccatcggtt gtccaaaaac gcttgcgcgg ccgcttgcaa
      241 atactccttg aacggcgctt cctcagcccc caaaccgtcc gaggccaagc gaagctcctt
      301 ggataccttc tccgataaat cctggaacgc ctccgtcagc ggctctgtca ccagcttatc
      361 cccttccttt ttcaccacca cgaacggaga catcagcttt tcgccatccg cctgcttcgc
      421 caacttctcg caaaacccct cctcgctttg catttcctgc ggatacatcg ccaccggtat
      481 cttgtccgga agcttcggaa tcgcagaaca tgccgggttt ttctccgtct tgggcgcccc
      541 gcacttcggc ccccaattac gacgaaacgc cgcgtgactc gatggatcat ctttcgggat
      601 ctgcgtctcc aaaggcgcca atccccgctg catgccgtgc aaatcatcga tgagtttggc
      661 cgcggcgagc atgtggcgaa tcaatacctt gtcctgcggc gaattcttcg tgaagtcaga
      721 aaataccaag gtcggctgcc cctgatcgag ttcttcgatg atgagctgtc gacgcgtttt
      781 ttccgcatcc gatagatctt tgggaaatgc aggtgctttg gcccactgct gcatctgtcg
      841 ataaacctcc tcgaaacgtg ccgtgaatcg ccccccatcg acccaattgc cctgggtcgg
      901 atagaattga agcgaggcta cttcgttggg gtcgatcgcc ctgttgccat ttttatcctc
      961 gatccaaaaa atcggtatgt ccatctgcac cgcaacgcgg ttgaaggtgc cgcggtcgat
     1021 cgcattgtaa acgaccacct tcggagcaga gacggaaggg gaaggggaag aggaaacggt
     1081 gctcgatgtg gtcttgggtt gatcacacgc cgaaaagact aaccaagcgc cgatccccat
     1141 catcgatcgt aatatgtagg tctttttcat gggcttctcc agactgtgcg tggatgagca
     1201 cgttactgaa gatctccgaa gaagaaagag tcgagacgag aataaactgg caaaacgtaa
     1261 ccacggatcg tatgttcagt atatgcctgc cccagccttc gccccctttg ccgccgccca
     1321 tatcactgct cctggatcat ccttacccct gccagctctt ggtcacgctt tgcccgatgg
     1381 cggattgccc cgcggtgccg tcgtggaatt gtctagccac ggcagtagcc tggcctcctc
     1441 cctcgcgctg tacgcctgcg cgtgcgccca acaacaaaca tggctgaaaa gcggacaacc
     1501 cgcctggtgc gcttggatcg accccggcca atccctctac gctcccggtg tcgccgccca
     1561 cggcgtcgtt ctcgaacgtc tgctcgtcgc ttatcccctc ccgcataccc tgccgcaaac
     1621 tgccgtccgc atgaccctca gccaagcttt tgccaccttg atcatcgata cccaaggcgt
     1681 tgtggggttt cctttgccct tatccctgga tcgatggccc aatatcgtgc gacgcctctc
     1741 tttggctgct cgcgatggaa gcacatgcgt gatcttgttg acgcagcgaa gccgctcgca
     1801 acatctcggg cttcctgttg cgttacgctt ggaattgaag cagcccaaac ctggcgtgct
     1861 gcatgtcgag gtggcgaaag aacgacaagg tcgtatgggc gcttgcgcgg aattggcgtg
     1921 gacccatctt tccaatccct ggtgctccca atccgcgtga aataggtcag agcaagtgct
     1981 tgcgcgctat ctctgcccac gttccatgcg gttcgagctg cacgtacttc tgccaatgcg
     2041 gccgagattg ctgctcgtct cccagatgtt cgaacgccat cgccaagttg aaatacgcat
     2101 cggcaaatgt cggatccgat gcaattgcag cctgaaaata ggtaatagct tcgcctggct
     2161 ccccccgctc gagcatgatg tacccaaggt tgtaatgcgc ttccggttgc tttccgtcga
     2221 tctccaacgc ccgacgatac aaccgttcgg ctgccttgtc gttgcagcga cgaaaacaaa
     2281 tattgcccca attcgtatag gcaatcgcca aatagggatc caactccaaa gcctggcggt
     2341 acaactcttc ggcctcatcg taagtcgccg gactttcgtc gagtcggctc gctcgctcat
     2401 agagttcgta ggccgattgc gtacgttgtc ggctgacggt cggacgaagg acccgcacga
     2461 catcgtttct caacgtttcg acttcgaagt ccaaaagcat ttgacccgtg ctcggctcat
     2521 aagccccgtc ttgcccacgg acgatgactt tggtgccgtc ggataccaca cgcagttcgg
     2581 acagaggacg tatgaccttg ggaagcgtgt gtttgagctt ggttaccgca cgggccacat
     2641 cgcgcaaacg aacgttgtgc tcgagaaggt gactggccgt ccgcagcgct atcaagtcac
     2701 tgaatgtgta ggctcgacgt ccccggcgcc gtccggatgg ggatacgacc cccgttcgat
     2761 ccagggaacg gagttgacct cgcgaaagcc ccagcaatcg acagacctcg gtgcccgaaa
     2821 acaaatccgt aatgggctcc cgcgcggaag gtaaaggcgg caaatcctga tcgggcgtct
     2881 gaggcaggtc atgtggcaga cgcctcgaca tgccgaagtc cacatgaatg accttgtgtg
     2941 ggtccaaatc cgatttttgt tttcgcccca tcgtttcgac catcgtgcga cgataccgac
     3001 aactgcatgg cctgcgcggc cgcgtcaaga ggctcttagc caaaccaacc cccaattcga
     3061 ggacaagtcg tggtcatgac ttacggaaaa tcgaaaaacc tcattgaaat cgcttaaata
     3121 ccaatatttc aggcttctcg tcccatgcga ctcgcccgat gctctaccac cgcgtctacg
     3181 tcgatatcga ttcgaagcgc tcttgcttcc gcgtggacaa atagatccat ctgcagcggt
     3241 ccaagcatcc ccggagcccg agcttgcgct tctcgtctcg caattctcat cgctcgaaga
     3301 cgtaaatgag gatcgatgat catggcgcgc cccttcgaac catgagtcac caaagccatc
     3361 gtgtccgaac gcaccatgat gctggtcgaa cctcgataga cgagagatga accttccgga
     3421 ctcgtcgctc cttccgaaat gacatcggcg tcctccaaca gggaagacca gaacgcgtca
     3481 cctacttgaa cgaaggatag gacacggacc gtttccggat ggggatgcct gaccttccgg
     3541 atgccgggac cttgccatgg attcatgggg ggatcgtaac gaatccacgc tggctccggc
     3601 caaacaggat gctcgaaaac cagcaaactt ggccaatcgt ctctccccgg acaacccttg
     3661 gaccacccag cgtcaggagt gaccaggaat ggcaaaacag ccggaaaact acagaggttt
     3721 ggatcgtcgc cgtcaccgaa tcctcattac cgaaaacacc gaatatcact tccgtgatgg
     3781 cttatgtatc gccgtccgtg accgacaaac cggcgaatgg atgaccgacc atgtcgcctt
     3841 gttgcgtccc gtatccggaa gcctgcagat cctacccaat ggagccatcc gacccaatat
     3901 cggtccccct cgcatcggcg aagccctgtt ttttgccacc ggaggacgag atctcatcac
     3961 cagtccgctg ctgcaggaaa aacgcccttc gaaacacgtc gtggacgctt accccgcggg
     4021 tcttcgcgga ttgcgcaggc gattttccca ccactgacgc atccccgcca gcgcctcacg
     4081 tctttccttg ctcgacaata cacgggtttt gcgtccaaat ggttgcatgg cctgcacgtc
     4141 ccaacgcctt ctcgaacttc tgaacgaagc aggattccgc tactggacag ctccgaaccc
     4201 agaagacgga tgtctcgtgt cgttcatggg caaccacgct acccgcccga ccgtcttcgt
     4261 tcacgttcgt gtggactccg atggttccgt gctcaccgcc atggtgaccg cctttttacg
     4321 tgtggccgac catccctcgc tgcccgtact catgcaggcc atgctccatg aagcctatct
     4381 gaccgtactt ggacaatggg aaatggatcc gagcgatggc gaagtgcgtg tcacggtgca
     4441 gctccccctg ttcgatgcgg agcccacggc caatcaggtc gctcgcacgg tgacggcggc
     4501 cgcgcgcctt gcggaccggg cctggccgcg attgctcgcc attctcgaaa cgggacaaga
     4561 ccccgtgctc gaggaagacg atgacgatcc agctcgtaaa gagttgcgac gtctccagat
     4621 tcgtatgcag gagctgctgc aggctcagcg atcaaaaacg gaccagaatt gaggcaactc
     4681 catgggcact acgatccaag aaatcacggc cattttcaag gaagcgaaca tccacttcga
     4741 tgtcgacgaa gaccttgacc tgattcgaag cctgtggagt cgcggcgaat cgaccgtcac
     4801 cttggtggtg ttcgttctcg aagacgggga actcgttcag cttcgtgccc ccaccctgtt
     4861 gcaggtcgac gatgacgcca acaaaccgtt gttatttcgt gcgatgctgc aaatggccta
     4921 tgaaattcgg ctcgttcaat tcgagtatga cccagccgac ggtgaagtga gcacatgcat
     4981 cgatatcgcg ctcgaggacg gaaagcttac gtctgcgcag cttcttcgct gttgcgccgt
     5041 gctgttggat gtggcgtttc tcgctcacaa acgtctcacc acgatcttgg aaacgggcaa
     5101 agatcccgct ttggattccg aagatgtgga tgaagaagaa gccgagcagc gccaatccca
     5161 gctcgatgcg atggcaaaac tggcggcgtt gattcgacgg ggtgggggag gagaggcctg
     5221 aatatcagct tttcgaagtg atacgtgcgg caacgctggc tagatccaca ccggtggccg
     5281 ctttgagttg ctcgttggct ccaatgattt tacgagccaa cggctcctgc tcccctgagg
     5341 ctcgtggaag caccgtaacc ttggcgatgc gaaccggatg ctctgctccg ctgatctttt
     5401 gaatcatggg caaaatattc tgaagaacca gcacttcctt ggctgccgct cctccctttt
     5461 tgtaggcctc cacgagttga tgcaacgccg ctgcttccgc cttgccgcgc tcgacgatcg
     5521 ctgcggccgc gcctaccgct gcctcctcca tttgtagccg agccgcatcc gctggttgca
     5581 ccacatcggc ctcgagctga cgctccacct gcaccgcccg tgcttgttgg cgcttgatct
     5641 ccgccatcac tttcgcaatt tgtgcccgca cttctccttc cgccgtggca atcatcgcat
     5701 cccgtcgcgt ccgcgcatcc acgatgcgtt tggccatctc ccgctccgca atcgctatct
     5761 ccgcatcgta acgggcaatt tccgatgcat tccagttcaa cgccttctgc tccgccgcac
     5821 ccgattgcgc ttgggtttcc gcaatgaccg ctcgctgccg aagactcgca ccgcggattc
     5881 gaccaatcga attgaggtac cccacgtcat ccgtaacgtt ctgaatcttg agttgatcca
     5941 gcttgatgcc gagcttggac aggtcgtgct cggcttcctc cgtcagaata tgcgtgaaac
     6001 ggtttttatc ctcattcact tcctccgggg tgagctgcgc caacacccca cgcagattgc
     6061 cttccagggt ctctttggct atatacatga tttcattgcg gtttcttcca aggaaacgct
     6121 cgactgcgtt gtggatcatc ggttcttcgc cgggaagctt gacgttggcc accgccttga
     6181 cgttcaacgg gatgcctcct ttggaaaagg cgccacgaac ctccacgtcg atgttgatgt
     6241 tgcgaaggtc gatgaaatcc acccgctcca acaacgggat tctcagcgct cggccgccgc
     6301 aaacgacccg gaaaccaagc actttgttgc caatttttcg cttggctccc gacaggatca
     6361 tcgcctggtt gggcggaacg acatatagca ttcgcttgat cgtgatgaga atgaacgcgg
     6421 caaccacaat cgccacgaaa aacaaaacgc cgatgatgcc catcacccac ctgcctttcg
     6481 ggactgaagc atcgcaccca ggtccacccc caccgcttgt cccgtctcct gcatgatctg
     6541 actgacaacc tggggaaaag cagccacaaa agagccataa ctggccgcat ccccgccatc
     6601 gaccacgttg atctctttga tttgcgtcga ggctgtcctt tgtaccgcag cctcgaccag
     6661 ctctttgaga tggttcaaaa cgtaaatctc gcgcccttga agcccagcgg ccgtccattc
     6721 cgccacgacc gcttgcagcg ctatcgcacg ggctttgccc gtttcgatga acggtgaagc
     6781 ttctccgcga gccaaaagct cttccgctcg gcgttttgcc tgggcgggaa gaacgacatc
     6841 gcaggcgaga cgaagctgct cgtactgctt gcgcatttct tgaagatttt gctccgccat
     6901 ggaacgagcc gtctccgcat cgaccgtggc ctcgttttct gccgaggcgg cttcggcttc
     6961 gagcttggca agctgggttt gatactcgtt ctttttcttg agaacctggg cttccgcatt
     7021 ttttagcgcg gtctcagccc gggttcgtgc ggcagcctgc gcctcggcaa tcgtttggtt
     7081 cgccgcgttc tcggcgttct ccgcatcgcg aagcatgttc gcaatggctt cacgccccaa
     7141 attcgtcaag tacccctgct catcggacac atgctggatt ttcagaacgt cgagctgcag
     7201 acccaaccgt tcgaagtcgt ctcccgcatt gctcaacagc ttgtccgcaa acttgagacg
     7261 atcctggttg acctcttccg gtgtcaattc cgcgagcact tctcgcagcg ctccttccag
     7321 cgtgtccttg gccacctccg ccaaacgctg gaccggaacg tcgaggaacc gttccgccgc
     7381 gcttcggaga aagcgtgggt cgctcgatat cttcacgttc gcgatcgctt gtaccgccaa
     7441 cgggatgccg cctttggaaa aggcgttttg aaccgttacc tgaacgggaa aaatccgcat
     7501 atccagctca gataccgtct cgagaagcgg aattctgatt cccctgccac catgcaagac
     7561 ccgatatccc accaccgaac catctggaag cttatgcttt cttccgctga aaatgaggac
     7621 cttgttgggt gggcagatga gcaacaaacg ctgaatgatc agcagcatcg cccccaataa
     7681 gatcagagta aacaccgatg ccccaagtac gatcgcactc agggcggctc cttccacgcc
     7741 tgaactctcc atcatgggac aagagaatac acggcttacg ctcgctgcca agacatgacg
     7801 cttccccggt taccgagccg gtgacctgct cgtgtcgata tcccccggaa atcagaaaac
     7861 caccatccta ttcggatggc tccgatggct tgccgatggg ctccacgcaa gcatattccc
     7921 cgcgatactc caccaccacc acttcatccc ccgtctcgat agaatccgcg tcggtgatgg
     7981 ctaacatatc gacttgctta ccttttacca tcacacggac ctttccgagg gagccttttt
     8041 tcaccggtat cgtgaccttc gccatttttc caatggtgtc ttgctcgccc atcgtcgacg
     8101 acacacttcc tcgaagcgcg cgaaacacaa ggcttgccgc cagacccgac aaaacaccgg
     8161 aagccacgga tagcccgagg gataggccaa accccgccat atcaaagtaa tgcagcaacg
     8221 aaccgaccat gccgaacgcc atcaaggcaa acgtccaaaa acgcatcgat agaaaaacac
     8281 cggccgcgcc atacgccccg ccgccatgcg cgtcgtccag ctcatgcgta ccatccgcgc
     8341 cgtcccctcc ccccgcatcg gcgtccgcac cccccgcatg cgaaagaata aattgaaggg
     8401 ttagggttcc cagtccaagg atgagcgccg caagatagac gattcccacg ccctcgagct
     8461 tatcgctttc atcgccccaa cgaaaccgct tccttgcgtt ccgccctttt gtaacgccta
     8521 tccacccgtc gcccgttgta ctagcgttgg tggaaccagc gagaagccaa cgtgttcgaa
     8581 atccactccc ccttgccagc cgtggacccc agtcgcgtgg aaaaagcgct acgtttgttg
     8641 caccgctcgt tgggcccctc caaaatcatc acctccaaag acggttgcga acctttcgcg
     8701 agggatgagt cggaggccgt aggccgcacg cccgacgccg ttgtcttggc ttccaccccg
     8761 aaggatgtgg tgctcaccct cgattgtgcg cgtcgagcag gcgttcccgt gacgcctcgt
     8821 tgtgctggaa cgggcaggac tgggggtgcc gtacctttgc gcgggggaat tgtgctgagt
     8881 acgttgggca tgggcgatat caaggaaatc aatcatcatg atcgggtagc cgtggtgcaa
     8941 cctggtgtaa ttcttgaaca atttcatgat gcggtggagc gggaaggcct tttttacccg
     9001 ccggatccca actctcgtgc ttcttgcgga cttggcggaa atattgctga aaacgcgggg
     9061 ggcccaagag ccgtcaaata cggggtaacc cgggattatg tcctcggtgt cgaaatggca
     9121 gcgatgaccg gaccgttgat tcgcatgggg cgacgaacgg tcaaaggggt ggttggctac
     9181 gatatgaccg ggcttttcgt gggcagtgag gggactttgg gggtggtgac ggaagccacg
     9241 cttgccctgc ttcacaaacc agatatggtg ttgactttat tagcaatttt tcctacaccc
     9301 cacgaggcgg gccgggcggt ggcggccatt tttcgatctc ggatttgccc acgatgtgtg
     9361 gaactgatgg atgccggcgc gctgcaagcg gttcgggcac aaggggttgg catcgaccct
     9421 ttggcgggag cgctgctttt gatcgaggtg gacggccatc ccgcggtggt cgccgaggag
     9481 gccgaggggg tccgcaagat cctcgccgag gccccggccg acgaggtcca cgaggcgaaa
     9541 aacgcccagg aggcggccgg gctggccgcc gcgcgccgca ccgccctgtc ggcggtggcc
     9601 cgcgtgtcgc ccacgaccat cctcgaagac accacggtgc ccaggagcca cctggccgag
     9661 accttcgcgg agatcgagcg gctcaaggcc aagtaccagg tccgcgtcgc caccttcggc
     9721 cacgccggcg acgggaacct ccatcccacg gccgtcaccg acgagcgcaa ccgcaaggag
     9781 atcgagaacg tccacgcctt cttcgcggag ctctacgaga aggtcctggc aatcggcggg
     9841 acggtttcgg gcg
//
LOCUS       DAOSOR010000165         9826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6884_length_9826_cov_4.900931, whole genome shotgun sequence.
ACCESSION   DAOSOR010000165 DAOSOR010000000
VERSION     DAOSOR010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9826
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6884_length_9826_cov_4.900931"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..373)
                     /locus_tag="PK710_10735"
     CDS             complement(20..373)
                     /locus_tag="PK710_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOT10239.1"
                     /translation="MACELGWRSTDERVTLGFMDPRLRVMVAEGALERSSYYDLLRVA
                     PGGDPSSLQRAFHEFALAFHPDRHAHEDTTIQTAAKKVFERGVEAYTVLRNPTAAQLY
                     DKRLQEGMLRLPPRR"
     gene            487..1002
                     /locus_tag="PK710_10740"
     CDS             487..1002
                     /locus_tag="PK710_10740"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOT10240.1"
                     /translation="MNLQEALNIALDYERKVRDHYVNGMKAIDDPQGKKVFKTLAREE
                     QGHVDHLTRCLKQWTEKGIVDPESLTPVVQPGLAWIEKAKDELKTKHETREASANEIE
                     LLKVALELEMTTSAFYRDLIGKLPEQDQSLFADFLEIEEGHVAIVQAELDAVQGNGFW
                     FDLMEFSLEAG"
     gene            complement(1029..3140)
                     /locus_tag="PK710_10745"
     CDS             complement(1029..3140)
                     /locus_tag="PK710_10745"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOT10241.1"
                     /translation="MAEVQADFNTTKKILDSAGIPIVGLAACSREEALRVADEIGYPV
                     VMKLISPDIIHKTDVGVVLLDIADRQEAECAYDCIIENATRAGSKQIDGVLVQKQAKR
                     GFELLVGARQDAVFGPVTMVGYGGRFVELFRDVAPGVGVLTHSDVERMLEQTMAGRVI
                     EGFRGPALDKRAIIDLAINVSKLMASHPEIRELDLNPVIVYPEGFSIVDARMILGDPI
                     IHPRAEDLSEARLTSLKSIFDAKSVAVVGASRPGTIGGIILKNSSRLDKLYPINPRRE
                     TLMGLPCYPNISAIPEPVDVAVFAVPPHDTIRGFQEFCERGGKGAVIVSDGYAEIGRP
                     DLEEQLKAISEKHGVVYIGPNGLGLFDSFTGFNTLFLPLVRSQLPSRSGPIGIISQSG
                     GIGLELLEMAAEDNLPIGTWVSVGNASGISIPELLEHMGRDDRIKIIAVYMEGLRNGL
                     QFMEVGRRVAQKKPVIVIKGGMAGGAQATMSHTASLAGSFEAFKAACRQAGFFLLEEL
                     TEDPKILINILSILTTQKAAKNNRVGVVSVGGGAAILLADQITSHGMRLTEFEQDTKR
                     RLSELLIGKIHVANEEEKERIAARVAANPLDLFGDANDDRLLEAIRILNDDPNTDVIV
                     AGLYLQVPYLSEYIGERLADLQRELSKPLIISPRGLSPYVMRMRSYMTQHDVHTYTVP
                     TIKPLSIAIDIWRRYGTDFTV"
     gene            3288..4256
                     /locus_tag="PK710_10750"
     CDS             3288..4256
                     /locus_tag="PK710_10750"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HOT10242.1"
                     /translation="MGRIIMRGTLVALIAGLCLSCGDDENTSESSGGNAGTNTNTGGA
                     SAAGASGGPSAGNGGAGATGGTGGAGETGGSGGSGGSGGSGGASIWQPPPKTSWQWQL
                     TGTIDTSVDVTMYDIDLFETSQATIQALQQQGRIVICYFSAGSWENWREDANSFPSSV
                     LGKTLDGWPDEKWLDIRSSEVRNLMKIRLERAVTKGCDGVEPDNVDGYANDSGFPLKA
                     SDQLDYNRFLAKEAHDRGLSIGLKNDLDQVSALVQDFDWALNEQCLEWDECDALLPFI
                     NANKAVFHVEYGNASLANTVCPKTAPLKFSTLIKKLDLDAWRVACP"
     gene            complement(4258..5709)
                     /locus_tag="PK710_10755"
     CDS             complement(4258..5709)
                     /locus_tag="PK710_10755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT10243.1"
                     /translation="MTGTVLVIEDEYGVRTGIEQILRMEGFEVWSAACAREAIDHLKT
                     KTFDVALIDYQLPDVDGLTLLHQIQQHDGLTKTCMITAYANIDMAIAATRQGVDFFLP
                     KPFTPEDLVGVVNNLLKRRHVEIEAARLREAHEASLIALASEKSQTHSLVSCLRDAVL
                     VVNAAGHVVLVNPSMARLLGSNAEQVVGQPVMDVLAAPAFGPLREQLETPEEKRGVFE
                     CEIDDRAHMVRVVVFRDEEGTARGSIVTLSDISEVRRLAVEKSRFLRTLVHELRSPLG
                     AIRSLLEVVMDRSLGEEMNAYDSFLQRADARIENLVGMVGQLLTLSRVDEQRTTESCE
                     PMAPAQVVEEVAKLYEDQARSRGITIRCTLSDGDERVRLDRDEWQTIVGNLIGNAVKY
                     NKPNGMVEIVLQREQGWLRLDVRDTGIGMSSDGARRVFEEFYRERRAETRDTEGNGLG
                     LSIVQRLVQRKGGSIDVSSVLGEGTSFCVRLPV"
     gene            complement(5706..6128)
                     /locus_tag="PK710_10760"
     CDS             complement(5706..6128)
                     /locus_tag="PK710_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT10244.1"
                     /translation="MSHTIVFVDDDRDFLAAQSAFFAARGYVVHTAEREDRALELLHE
                     ITPNVIVLDLMMERADTGVALSHKIRQIEALRAVPIILLTGVVAATGRRLDLDVAALR
                     RWAGIDEYLDKPVTARQLLRAVESLIHRSNQDSASEEA"
     gene            complement(6125..8161)
                     /locus_tag="PK710_10765"
     CDS             complement(6125..8161)
                     /locus_tag="PK710_10765"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOT10245.1"
                     /translation="MNAAPVHEGRGEESGNVISTVLGKCRRCFTCVRACPAKAIRFEQ
                     GQARVVEERCIACGNCVVVCAQHAKEYRSGLEKTLELLGGEQPVAALVAPSFPAEFFE
                     LDAQRLVGALRKLGFAYVVEVAHGADLVSAAYQRQMPHRKGPCIASACPAVNEYIRKY
                     APHLVEKLIPVVSPMIATARAVHAVYGEHVRCVFMGPCVAKKREAWDPQVAGEVDEVL
                     TFREVRSLFERQGIVLDQQQDSDFDPPRAGLGRLYPLVGGLLKAMGHERNLLQPEAIV
                     ISGHQETLEVLGKLSGDESFASLIEPLMCRGCYSGPGMSPGRQRFERKQAVVAYVREQ
                     MKRGSEQDLAPSGDESISLSRVFVPDERRLAEPPEAELRRILAQTNKFSPSDELNCGA
                     CGYATCREKAVAVYHGLAEDAMCLPFMLEAAERVCEELKLPWLELREVQSHLVNSEKL
                     VSLGQMAAGIAHELNNPLGTILLYAGILRKKLGNREDLRHDLDLLSDESQRCKRIIGS
                     LLDFARQNRVKLESEKVARLFRDAVETSLANRDPNLQVRVEVSDDLQVEVDRDQLRQV
                     LDNLCKNAAEAMEGRQGHIVLRGRDGPREGTVILSVSDQGCGMSKEAQDMIFQPFFTT
                     KRIGKGTGLGLPIAYGIIKMHHGRIWVESEIGVGTTVFIELPRHRAQVARSVIE"
     gene            complement(8170..>9826)
                     /locus_tag="PK710_10770"
     CDS             complement(8170..>9826)
                     /locus_tag="PK710_10770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HOT10246.1"
                     /translation="LTAAKRAGIEIPSLCVLPCASTPAGACRLCLVEVEGESRLQTSC
                     TLPARDGLVIRAHTPRLLTIRRHIVELMLSNAKHDCAVCARQGSCELAHLARRLGVQQ
                     RRFSGIERTWPIDISSPALTRDPNKCVLCGRCVTVCHQGQGVGAIDFAGRGFTTRVSP
                     GFHSSLNGSACVFCGQCTRVCPTGALMEKSHVEGVVQALADPDCFVVAQIAPAIPATL
                     AEGGPEEPLATTLERLCDVLHRIGFHAVFDTTFAADLTVVEEATELVERIRHGGVLPM
                     FTSCSPAWVHYVETHAPHLIAHLSTCKSPQQMAASLIKRVLPSRLDPKPRRIVSVSIM
                     PCTAKKAEASDQGEVDFVLTTRELIKLMERFGIDWARSVGRRPLDSPFADSTGAGRLF
                     GGTGGVMEAAIRTVHKLITDKDLRDGWKIPGARGMGPIRDFSLNIEGLDLGFAVVSGI
                     GHVPALLKDIEAKRLSVHFVEVMSCPGGCVAGGGQPYDTNVDSVQQRIARLQEADRRA
                     RQRGSHTSPAIAALYEQVFGVAGGEASHRLLHRHYVDRSVEEG"
BASE COUNT         2232 a         3041 c         2533 g         2020 t
ORIGIN      
        1 gtgctccggc tgcgggacgt caccggcggg gaggcaggcg cagcatcccc tcttgcaagc
       61 gtttgtcata gagctgcgcc gcggttggat tgcgcaagac ggtataggct tccacccctc
      121 gctcgaacac tttttttgcg gccgtctgga tggtggtgtc ctcatgggca tggcggtccg
      181 gatgaaacgc caacgcaaac tcatgaaagg ctcgttgcaa ggagctgggg tccccacctg
      241 gcgcgacgcg cagcaaatca tagtaggaag accgctctag cgctccttcc gccaccatga
      301 cgcgaagcct tgggtccatg aatccaaggg taacccgctc atcggtcgaa cgccatccca
      361 actcgcaagc cattaaccta cgaaaacccg atttcccggt cgactcgatc gagcagttcg
      421 gagtatcaac gatagacgct cgcgttttat cgagactcga cgttcgaaaa cccacaagag
      481 ggtaaaatga atcttcaaga agcgttgaat atcgcgctcg attacgagcg caaggttcgg
      541 gaccactatg tcaacggcat gaaggccatc gatgacccac aagggaaaaa ggtcttcaaa
      601 acactagctc gtgaggagca ggggcacgtc gaccacctca cccgttgtct gaaacagtgg
      661 acggaaaaag gcatcgtcga tcccgagtcc ctcacgcctg tcgttcagcc cgggctcgcc
      721 tggatcgaaa aagccaaaga tgagttgaaa acaaaacacg aaacgagaga ggcatcggca
      781 aacgaaatcg agcttctcaa ggtggctctc gaactcgaga tgaccacaag cgcgttttac
      841 cgcgacctca tcggcaaact ccccgaacaa gatcaatcgc tcttcgctga ttttctcgag
      901 atcgaagaag gtcacgtcgc catcgtgcaa gcagaactcg atgccgtaca aggcaacgga
      961 ttctggttcg acctcatgga attctcgcta gaagcaggct gagtggcccc tcgccccacc
     1021 caaccgcgtt agaccgtaaa gtccgtcccg tatcgacgcc aaatatcgat cgcgatggac
     1081 aggggtttga tcgtaggtac cgtataggta tggacgtcat gttgcgtcat atacgatcgc
     1141 atgcgcatca cataggggga caagccacgg ggcgagatga tcaaaggttt gctcagctcc
     1201 cgttgcaggt ccgccaaacg ctctccgata tactcggaaa gatagggaac ttgcaggtat
     1261 agcccggcca cgatgacgtc cgtattcgga tcgtcattca aaatccgaat ggcctcgagc
     1321 aagcgatcgt cattcgcgtc accaaataaa tcgagcggat tcgcggctac ccgcgcggca
     1381 attcgctctt tttcttcctc gttggcgaca tgaatcttgc ctatcaaaag ttcggacaag
     1441 cgacgcttgg tgtcttgctc gaattccgta agacgcatgc catgggacgt gatctggtcg
     1501 gcaaggagta tcgcggcgcc accgcccacg ctcactaccc ctacacgatt gtttttcgct
     1561 gccttttgtg tagtgagaat cgataggatg ttgatgagta tcttggggtc ttccgtaagc
     1621 tcttcgagca aaaagaatcc cgcttgccga caggctgcct tgaacgcctc gaagctgccc
     1681 gcaagagatg ccgtgtgaga catggtggct tgcgcaccgc cagccattcc tcccttgatc
     1741 acaatgactg gttttttctg agccacacga cgcccgacct ccatgaactg aagtccattt
     1801 cgaaggcctt ccatatagac cgcgatgatt tttatccgat catcacgacc catgtgctcg
     1861 agaagctcgg gaatggaaat accgctggcg tttcctaccg atacccaggt cccgatcgga
     1921 agattgtcct cggccgccat ttcgagcagt tccaaaccaa tgccaccgct ttgcgaaatg
     1981 attccgatcg ggccgcttcg cgacggaagc tgggaacgaa ccaagggcaa aaagagcgtg
     2041 ttgaagcccg taaagctatc gaataggcca agtccgttgg gacctatgta tacgacccca
     2101 tgtttctccg aaatcgcctt gagttgttct tccaaatccg gacgaccaat ttcggcgtag
     2161 ccatcgctca caatgaccgc gcctttgcct ccgcgctcac agaactcttg aaagcctcga
     2221 atggtatcgt ggggaggaac cgcgaatacg gctacgtcga caggttcggg aatggcgctg
     2281 atgttggggt aacagggcaa tcccatcagc gtttcgcgcc tcgggttgat ggggtatagc
     2341 ttatcgagtc ggctcgagtt tttgagaatg attccgccga tggtgccagg gcgtgaggca
     2401 ccaacgacag cgacggactt ggcatcgaag atggacttga gactggtaag acgagcctcc
     2461 gacaagtcct ccgcacgcgg atgaatgatg ggatctccca aaatcatacg cgcatcgaca
     2521 atcgaaaacc cttcggggta gacaatcacc ggattgagat cgagttcgcg gatctccgga
     2581 tgcgatgcca tcaacttgga tacgttgatg gcaaggtcga tgatggcgcg cttgtcgaga
     2641 gccggtcccc gaaatccctc gatcacacgg cccgccatcg tttgctcgag cattcgctcc
     2701 acatccgaat gcgtaagaac accaactccc ggcgccacat ctctgaacaa ttctacaaat
     2761 cgaccaccgt agcccaccat ggtcacggga ccaaatacgg catcttgtcg ggctcctacg
     2821 agtaattcaa atccacgttt ggcctgcttt tgcaccaaca cgccatcgat ttgtttcgaa
     2881 ccagcccgcg tcgcgttctc gatgatgcaa tcgtaggcac actccgcctc ttgccggtcg
     2941 gcgatgtcga gcaaaaccac tcccacatcc gttttatgaa tgatgtcagg agaaatgagc
     3001 ttcatcacca ccggatatcc gatctcgtcc gccacccgga gcgcttcttc gcgcgagcaa
     3061 gcggcaagac ccacaatggg aatgccggcg gaatcgagga ttttcttggt tgtgttgaaa
     3121 tcggcttgta cttcggccat ctcgttgttc cttggacagc agttgagcgc gctgacccta
     3181 tcacgaacgc cagccgtgag aagagcgctc agttcgaact tccccaagag tgaatcgttg
     3241 gcatatcatc cacccgtggg gcaggattac gcgacgaagg agcaatcatg ggacggatca
     3301 tcatgcgtgg cactttggtg gctctcatcg ccgggctttg cctatcgtgc ggagatgatg
     3361 aaaacacgag tgaatcgtcc ggtggaaacg ccggaacgaa cacgaatact ggaggagcct
     3421 ccgcggcagg tgcttccggt ggaccctctg cgggtaacgg gggagcgggc gcaactggtg
     3481 gaaccggagg cgctggagaa acgggcggtt ccggcggctc gggcggttcc ggcggctcgg
     3541 gtggcgcatc catttggcag cctccgccca aaacatcgtg gcaatggcaa ctcaccggaa
     3601 cgatcgacac atcggtcgac gtgaccatgt acgacattga tttattcgaa acctctcaag
     3661 ccaccatcca agccctacag cagcagggcc gtatcgttat ttgctatttt tccgctggat
     3721 catgggaaaa ctggcgagag gacgccaata gcttcccttc ctccgtactc ggaaagacac
     3781 tcgacggttg gcccgacgag aagtggctcg atatccgaag ttcggaagtc cgaaatctca
     3841 tgaaaatacg cctcgaacga gcggtaacca aaggatgtga cggagtcgaa cccgacaatg
     3901 tcgacgggta tgccaacgac tccggcttcc ccctcaaagc ctccgatcag ctcgactaca
     3961 atcgttttct tgcaaaagaa gcgcacgatc gaggcctgtc gatcggactg aaaaacgacc
     4021 tcgaccaagt ttctgccctc gtccaagact tcgactgggc gctgaacgaa caatgcctcg
     4081 aatgggacga gtgcgacgcg ttgcttcctt tcatcaacgc caataaggcc gtatttcacg
     4141 tggaatacgg taatgcgagc ctcgccaaca cggtatgtcc caaaaccgct ccgctgaagt
     4201 tctccacgtt gatcaaaaaa ctcgatctag atgcatggcg agtagcctgc ccttgattca
     4261 tacaggcagc cgtacgcaaa agctcgtccc ttccccaagc accgaagaaa cgtcgatgct
     4321 ccctcccttt cgctgcacca atcgctgaac gatggaaaga ccaagaccat tgccttcggt
     4381 atcgcgggtc tccgcccgtc gttctcgata aaattcctcg aatacgcgcc gcgcgccatc
     4441 cgatgacatc ccaatgcccg tatcccgcac atcgagccga agccagcctt gctcacgctg
     4501 caagacaatc tccaccatgc cattcggctt gttgtacttg acggcgtttc cgatgagatt
     4561 gcccacgatc gtctgccatt catcgcggtc caatcgtacg cgctcatcgc catccgacag
     4621 cgtgcagcgg atcgtgatcc ctcgacttct cgcttggtct tcgtagagtt ttgcgacctc
     4681 ttccacgacc tgggcgggag ccatgggttc gcaactttcg gtggttcgct gttcatcgac
     4741 ccgagacaac gtgagcaatt gcccaaccat acccaccagg ttttcaatac gcgcatccgc
     4801 tcgttgtaaa aacgaatcat acgcattcat ctcttcgccc aagcttcgat ccatcacgac
     4861 ttcgagcaaa gagcgaatcg ctccgagagg ggaccgcaat tcatgaacga gagtgcgtaa
     4921 aaatcgggac ttttccaccg ccaaccgacg cacttccgag atatccgaca gcgttacgat
     4981 acttccacga gccgttccct cttcgtcccg aaacaccacc acgcgaacca tatgcgcgcg
     5041 atcgtcgatc tcgcattcga acacgcctcg tttttcctcc ggcgtttcca gttgttctcg
     5101 cagtggccca aatgcgggag cggcaagcac atccatcaca ggttgaccca cgacctgctc
     5161 ggcattcgat ccaagcaacc gcgccatcga gggattgacc aacacgacat gccccgccgc
     5221 attgaccacc agaaccgcat cccgcaaaca acttaccaac gagtgcgtct ggcttttctc
     5281 cgaagccaaa gcgatcagac tcgcttcatg agcctctcga agccgtgccg cctcgatctc
     5341 cacgtgacgt cgtttcaaca aattgttgac gacacccacc aaatcctctg gcgtgaaagg
     5401 tttgggtaga aaaaaatcca ccccctgccg cgtagcggca atcgccatgt cgatgttcgc
     5461 atacgccgtg atcatacacg tcttggtcaa accgtcatgc tgttggatct gatgaagcag
     5521 cgtgagacca tccacgtctg gaagctgata gtcgatgagg gcaacatcga acgttttggt
     5581 cttcaagtga tcgatcgcct cacgagcgca tgcggccgac cacacttcaa aaccctccat
     5641 ccgaaggatt tgttcaatcc ccgtccgcac accgtattca tcctcgatga cgagaacagt
     5701 gcctgtcatg cctcctccga cgccgaatct tgattggatc gatggatcag cgactcgacc
     5761 gcgcgaagca actgcctggc cgtcaccggc ttgtccaggt attcatcgat tcccgcccaa
     5821 cgacgaagcg ctgccacatc gagatcgagc cgacgacccg tggctgctac gacgccggtg
     5881 agcagaatga tcggcacagc acggagcgct tcgatttggc ggattttatg gctcagagct
     5941 acgcccgtgt cggcccgttc catcatcaag tcgagcacga tgacgttcgg agtgatctca
     6001 tgcagcagtt ccaacgcgcg atcttcgcgt tccgccgtat gcacgacata tccccgcgcg
     6061 gcgaaaaaag ccgactgcgc cgcaagaaag tcccggtcgt cgtccacgaa tacaatggta
     6121 tggctcattc gatcacactc ctcgctacct gggctcgatg cctcggcagc tcgatgaaca
     6181 cggtcgtgcc cacgccgatt tcggactcca cccaaatcct tccgtggtgc atcttgatga
     6241 ttccataggc aatcggaagt cccagccccg tcccttttcc aatgcgcttg gtcgtaaaaa
     6301 acggttgaaa aatcatatct tgcgcttctt tcgacatccc acagccctgg tccgaaacgc
     6361 tcagaatcac cgtcccctcc cgcggtccat ccctgccccg caacacgata tggccttggc
     6421 gtccctccat cgcctccgcg gcgttcttgc atagattgtc gagcacttga cgaagctggt
     6481 cgcgatccac ctccacttgc agatcgtcgg atacctcgac ccgcacttgc aggttcggat
     6541 ctcgattcgc caaggacgtt tccaccgcat cacgaaacag acgcgccacc ttttcggatt
     6601 cgagcttgac gcgattctgc cgtgcgaagt cgagcaaact accaatgatg cgcttacagc
     6661 gctgtgattc gtccgagaga aggtcgaggt cgtggcgcaa atcctctcgg tttcctagct
     6721 tttttctcag gatgcccgca taaagcaaga tcgttcccaa aggattgttg agttcatgag
     6781 cgatgcccgc ggccatttga ccgagcgaaa cgagcttctc cgaattgacc aggtgacttt
     6841 gcacctcgcg caattccaac cacggcagct tcaattcctc acatacacgc tcggccgcct
     6901 cgagcatgaa cggaagacac atcgcgtctt ccgcaagacc gtgatacacc gccaccgctt
     6961 tttctcgaca tgtcgcataa ccacacgcgc cacaattgag ttcatcgctc ggcgaaaact
     7021 tgttggtctg cgccaaaatg cgtcgaagct ccgcttccgg gggctccgca agacgacgtt
     7081 cgtccggtac aaacactctc gaaagcgaaa tcgactcgtc acccgatggc gcaaggtctt
     7141 gttccgagcc gcgtttcatt tgctccctaa cgtatgccac caccgcttgt tttcgttcga
     7201 accgttgccg tccgggactc atccccggcc ccgaataaca accgcggcac atcaacggct
     7261 cgatgagaga agcaaaagat tcatcaccag acagcttgcc caatacttcg agtgtctctt
     7321 gatgaccgct gatgacgatg gcttccggtt gtaaaagatt gcgctcgtgc cccatggctt
     7381 tgagcaatcc tcccaccaac ggatacaacc gtcccaaccc cgcccgggga ggatcgaaat
     7441 cactgtcctg ctgctggtcc aagacaatcc cttgacgctc gaacaacgag cgcacttcgc
     7501 gaaaggtaag aacctcgtcg acctcccccg ccacttgcgg atcccatgcc tcgcgctttt
     7561 tcgccacaca cggacccatg aacacacagc gaacgtgttc gccatatacc gcatgcacgg
     7621 cacgagccgt cgcaatcatc ggcgacacga ccggaatcag cttctcgacc aaatgggggg
     7681 cgtattttcg aatgtactcg ttcaccgcgg gacacgccga agcaatgcat ggccctttcc
     7741 tatgcggcat ctgtcgctga taggccgcgc tcaccaaatc agccccatga gccacctcca
     7801 ccacgtaggc aaagcccagt ttgcgaagcg ctcccacgag tcgttgcgca tccaattcga
     7861 aaaactccgc gggaaacgat ggtgccacca acgccgccac cggctgctcc ccgccaagca
     7921 actcgagcgt tttctccaat cccgaccggt actctttcgc gtgttgcgca cataccacga
     7981 cacaattgcc gcaagcgata catcgctctt ccaccaccct cgcctgcccc tgctcgaacc
     8041 gtatcgcctt cgccggacag gcccgcacac acgtgaagca ccgacggcac ttgcccaaca
     8101 ccgtactaat cacgttgccg ctttcctcac ctcgtccttc atggacggga gctgcgttca
     8161 tgagtccttt catccttcct ctacactgcg gtcgacgtaa tgccgatgga gcagacggtg
     8221 actcgcctct cctcccgcaa cgccaaacac ctgctcgtaa agcgctgcaa tcgcaggact
     8281 tgtatgcgag ccgcgctgtc gcgcccgtcg atccgcttct tgcaatcgcg cgatgcgctg
     8341 ctgaaccgag tcgacattcg tatcgtaagg ctgtccaccg cccgccacgc atcctccggg
     8401 acaggacatg acctcaacga aatgcacaga cagacgtttg gcctcaatgt ccttcagcag
     8461 ggcagggaca tgaccgatgc cgctcaccac ggcgaaccca agatctaacc cttcgatatt
     8521 caaggagaaa tctcgaatcg gccccatccc tcgcgcgcca ggtattttcc aaccgtcccg
     8581 caaatcctta tcggtgatca gcttgtgaac cgttcggatc gccgcttcca tgaccccacc
     8641 ggttcctcca aatagccttc cggctcccgt cgagtccgca aacggtgaat ccaacggacg
     8701 acgcccgacg cttcgggccc aatcgattcc gaaccgttcc atcaatttga tcaactctcg
     8761 cgtggtgagc acgaagtcca cttccccttg gtcgctggcc tcggcttttt tcgccgtgca
     8821 gggcatgatg gagacactga cgatgcgacg cggtttgggg tcgaggcggg agggtagcac
     8881 ccgtttgatg agcgaggcag ccatttgctg gggcgatttg cacgtggaaa ggtgagcgat
     8941 gaggtggggg gcgtgcgtct ccacgtaatg gacccacgcc ggagagcagc ttgtaaacat
     9001 gggaagaacg ccgccatggc gaatgcgctc gaccagttcg gtagcttcct ccacaaccgt
     9061 gagatccgcc gcaaacgtgg tatcgaaaac ggcgtggaag ccgattcggt gcaacacgtc
     9121 gcaaagacgc tcgagtgtcg tggccaaggg ctcttcaggt ccgccctcgg caagggtggc
     9181 ggggatggcg ggggcaattt gcgcaacgac gaaacaatcc gggtcggcca atgcctgcac
     9241 caccccctcg acatggcttt tttccatcaa ggcaccggtg gggcaaaccc gggtgcattg
     9301 gccgcagaac acgcaggcgg aaccattgag actcgaatga aatccaggac ttacccgtgt
     9361 ggtgaagccc cggcccgcaa agtcgatggc tcccacgcct tgcccttgat ggcatacggt
     9421 gacgcagcgc ccgcacaata cgcatttatt cggatctcgc gtcagcgccg gcgaggagat
     9481 gtcgatcggc cacgttcttt caatccccga gaatctgcgc tgttggacgc caagccttcg
     9541 cgccagatgg gccagttcgc agcttccctg gcgagcacat acagcgcaat cgtgcttggc
     9601 attcgagagc atcaattcca cgatatgacg acgaatggtg agcaggcgag gcgtgtgggc
     9661 tcgaatgacc agaccatctc gagccggaag ggtacaactc gtttgcaaac gggattcccc
     9721 ttccacctcg acaaggcata ggcggcatgc gcccgccggc gtggaagcgc agggaaggac
     9781 acaaaggctt gggatctcga tacccgcgcg cttggcagcg gtaagg
//
LOCUS       DAOSOR010000166         9817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6894_length_9817_cov_5.099365, whole genome shotgun sequence.
ACCESSION   DAOSOR010000166 DAOSOR010000000
VERSION     DAOSOR010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9817
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6894_length_9817_cov_5.099365"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2259
                     /locus_tag="PK710_10775"
     CDS             <1..2259
                     /locus_tag="PK710_10775"
                     /inference="COORDINATES: protein motif:HMM:NF023478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HOT10247.1"
                     /translation="AHLNFNIGPTLFAWLQTHAPATYARILQADRQSCFLHDGHGGAI
                     AQAYSHAILPLCSVRDRNTQIRWGIADFRYRFQRPPEAMWLPETACDLPTADALAQHG
                     MRYLILSPHQASRVRHRNQNEWRDVSDGSIDPRLPYRLALPHGRSIVCFFYDGPLAHA
                     MSFEAMLDTSRGMAERLRAGAKDNAENQLVHVATDGETYGHHKRFADRALAYFIRKEA
                     EAHGLRLTTYGAFLEQQPVDDEVELKNGEGTAWSCAHGLGRWIRDCGCNAGRNPGFRQ
                     AWRGPLRDALDGLRDDSAVVFEQAAGQWLRDPWAARDDYIHVILNNTSEARQAFLDHH
                     ALRQISSHERVRIFEMMEAQRMMQLMYASCGWFFDDLAGLETTQIMKYAGMAARLLES
                     ATGQSLFENFIDRLALAHSNLEKEGNGADIYRRHVAPLEVPATRRVARFASSTLLDNT
                     SSQVADPAFEIESLDRLCFQQVSQHLVLGRVRLRRTRTERSTLFLYGATSLPERDMHC
                     GVIECNDEKHFERIVQQAEKVFEQPSTPAIIRLIDSFFAPHFFSVRELSDAGRAEFIE
                     HTVTDLLERLATTYGYLYDEHRKTMQTFERVGAEVPVELRLVAEYTLARRLDAALLEA
                     SDPTDPGLERTVRDIAHQAQELGIRLVVPRSTLRLRQWLDQAAEQLQTDGSTARAQHA
                     SALLSLTESLGIRIPIDHLQDAIFNHLNHRVAWPEELQKRLAQQVGVQHPPHSPRDES
                     HEPTHGLAKTEA"
     gene            complement(2269..2814)
                     /locus_tag="PK710_10780"
     CDS             complement(2269..2814)
                     /locus_tag="PK710_10780"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOT10248.1"
                     /translation="MINLRPATVSRLRDRLKQTGARPSIVISSDQDVLAKAGLLPPEE
                     RTAVQGIDPIVETMFLMMAADGTVGEDEYNVIRGAVRELTENSIRTTTLNAMLESYQQ
                     ALEKEGQQARIESIAHRLGDDEAGCESAFVLAAAVAFADEVIDDTENELLNTFAEAIG
                     LSEDRANELLDELEGDWQNDD"
     gene            complement(3161..4576)
                     /locus_tag="PK710_10785"
     CDS             complement(3161..4576)
                     /locus_tag="PK710_10785"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT10249.1"
                     /translation="MTTSHLPRRPRTALGIKVAKSVLVATCCFSACCAICCNDGDDGM
                     SDPDAVAGLTDGPRARAQRDAAEDIVNQADAFEQRDVWRQADAADDNAGDAVAKPSLD
                     GAAKDVVEEQNKQDDSSGRCPEGMVYVEGDYCMTVTHQCTDSLADKTAVGALGFDDQC
                     IAYVPGSSKCGTARKKVSVHLKVVPGDKHDQDRADTKHAFGKKFLGAKAGDVINGCTV
                     ERILGFGRDSAVPGSIVQLDCPSVIRLAFCMDRYEYPNQPGATPARLASWYEAQETCE
                     AAGKRLCEEQEWTLACEGPERQPYPTGWTRGIWTADTEESRSGCNISRCYSNCLPKDQ
                     DANGRCTEYDPLLKRQASRPACYTEPKWISQQLPGQSEETKKKYFDMVDRASGDPNYP
                     FLLPSGSMSQCVSPYGVFDLTGNVDEWVQSFDYHESKISNLKGGHYLHNCRARCRPVT
                     TQHGPFYVQASIGFRCCADPR"
     gene            4793..5761
                     /locus_tag="PK710_10790"
     CDS             4793..5761
                     /locus_tag="PK710_10790"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HOT10250.1"
                     /translation="MHTVIVSAPLPGTAVARLTTAGHRVIQGDQPGGLGHEGIIRVLR
                     DHPDTSALIPLVSDRVDARVLDAGPSLRIVANYGVGIDNLDLVELKRRGIVATNTPGV
                     LTEATADFAMALILDACRNVSRGDRLVRAGLWKGWSPTQLLGPRVTGSTLGIVGFGRI
                     GQAVAARARGFSMRVLYSDPIRVSPQIEKDLQATYLSLGDLLAQSDIVSLHCPSTDST
                     RGLMNETRLRQMRPGSVLVNTGRGTCVEEAALAKLLKEGHLAAAGLDVYEREPMIHQE
                     LLDLPNVVLAPHLGSADLPAREGMAELSVSSVIAVLAGNEPAHRVA"
     gene            complement(5817..9584)
                     /locus_tag="PK710_10795"
     CDS             complement(5817..9584)
                     /locus_tag="PK710_10795"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT10251.1"
                     /translation="MSRSSQEHQLSLPSRLEVRRKLGEGAFGSVYEVHDRELQSRVAL
                     KCLERLDAHSLFRFKREFRELADIVHPNLVRLHDLFCFDSRWCFTMELIHGVSFFDHV
                     RPGAAYPNDAPTIDSEDYQTAVSGVLGSGGTVNTTRLRSALMQLTEGVGALHRAGKLH
                     RDLKPQNVLVTENGRVVVLDFGLLADIDEKGEQEAATEIVGTPAYLAPEAAAGLVHGA
                     ESDWYSVGVMLYEVLTGRLPFDGKPVSMLIRKQMADPVDPEPLLEPNQRHFARLSMQL
                     LARRPQDRPKLESIQAALVAASDHDEDSEISSFRGEDVASLITYQPSEYLVGREASLE
                     LMSKAFENVVQGKGTMLRGYGRSGMGKTSLVRFFVDDLRRAKRARVLSGRCFECESVP
                     YKALDSLIDALTQWLLARSCDEVVTYLPKDILPLARMFPVLRRIPVIAAMGDGEELEG
                     LDYHEVRKSAFSALRSLFGEVSRRKPVVLWIDDLQWGDEDSAAFFSNLLQPPDAPRVL
                     LILSHRSEDTETSPILRQIVHAQTRAAYRCFEVEVSPLSQDACERLASLILREQGSHD
                     SALPFALASESGGCPLFVLHLVRHALRLDGGSSPPTGVRLDDVLWEYLGTLSAPAARM
                     LRVVALAGRSIELSVAREVAGLSGELREALDVLRTQRLIRTQGVRSTDLVEPYHDRIR
                     EIVTSRMSLEERVSLHQALARCLESRGADPETLVVHYRGSGDRESAWRCAVAAAERAA
                     EALAFRRAADLYREALLLKPERRTYELRIKLADALANAGHGADASAAYLEALVTAPAQ
                     DVLEIRRKAAEQALLVGKVDEGLALIDDMLAAAGMKLTKTSLGALLSLLWRRAWLRVR
                     GVGFRGTYHHDPEILRRIDVGWALAVGLSNCDTIRGADLQVRALLLALSAGEPSRIAR
                     GLALQAGMLAVAGPKCHDKCMELLDASRALTSKLGDPFTLGWYHVGAAAVAYFEGRFG
                     DCISEGDKAMAAFTHCSGVSWERTTIRHYAIWSLMWIGHVGAAAKRTKAQLEAAFERG
                     DLYSATDLRLFTSNMAWLVDDTPQEARRAVDEAMEHWSRRGFHAQHYYALYAYGQIDL
                     YIGDGESALKRVDQAWSPLRASMLLQVQTVRIEMLHLRARSALAAVHQCPERATKLLR
                     RAKSDAAELAREYSIVAPPMAELIRAAIASIENQSERCVACLRKAIEGFEAAEMGQYA
                     NVARRWLAIRSHDEAALAATTAYMESEGIQNIERWTQMLAPGFANPQDRPH"
BASE COUNT         2197 a         3043 c         2507 g         2070 t
ORIGIN      
        1 gcccatctca atttcaacat tggacccacg cttttcgcct ggttgcaaac ccacgctcct
       61 gcgacctacg cgcgcatcct tcaggcggac cgacagagct gttttcttca tgacgggcac
      121 ggcggcgcca ttgcgcaagc ctacagtcac gccattcttc ccttgtgttc cgttcgtgac
      181 cgtaacacgc aaatccgatg gggaatcgcg gacttccggt atcggttcca acgaccaccc
      241 gaggcgatgt ggcttccgga aaccgcttgt gatctcccta cggcggatgc gctggcacaa
      301 cacggcatgc gatatctgat tctttctccc catcaggcca gccgtgtccg acatcgaaac
      361 caaaacgagt ggcgtgatgt ttccgatggg agcatcgacc ctcggctgcc ttatcgtctg
      421 gcattgcccc acggtcgatc catcgtgtgt tttttttacg atgggccgtt agcccacgcg
      481 atgagtttcg aggccatgct cgataccagt cgcggaatgg cagagagatt gcgcgcggga
      541 gcgaaggaca acgccgaaaa ccaactcgtt cacgtggcga cggacggtga aacgtatggg
      601 catcataagc gttttgccga tcgagcgctg gcctatttca tccgaaaaga agctgaagcc
      661 cacggtttgc gcctaacgac ctatggagcg tttttggagc agcaaccggt ggacgatgag
      721 gtcgagttga aaaacggaga gggcacggcc tggagttgtg cccatggcct tggacgatgg
      781 atccgggatt gcggctgcaa cgcggggcgt aaccccgggt ttcgtcaagc gtggcgcggg
      841 cctcttcgcg acgccctcga cggccttcgc gacgattccg cggtggtctt cgaacaagcc
      901 gccggacaat ggctacggga cccttgggca gcgcgcgacg actatatcca tgtgatactg
      961 aacaatacgt cggaagctcg gcaggcgttt ttggaccatc atgctttgcg acagatctct
     1021 tcccacgagc gtgttcgcat cttcgaaatg atggaagcac agcgcatgat gcaactcatg
     1081 tacgcgagtt gcggctggtt tttcgatgat ttggcgggac tcgagacaac acagatcatg
     1141 aaatacgcgg gaatggcagc acgattgctg gaaagcgcca ccggccaatc gctgttcgaa
     1201 aacttcatcg atcgtcttgc tctggcccat agcaacctgg aaaaagaggg caacggagcc
     1261 gatatatatc gtcgccacgt ggcacccctg gaagttcccg ctaccaggcg tgtcgctcgt
     1321 ttcgcatcga gcaccctgct cgacaataca tcttcgcaag tcgccgatcc ggctttcgaa
     1381 atcgaatcgc tggaccgcct ttgcttccaa caagtctcac aacatctggt ccttggccgc
     1441 gtacgtcttc gtcgcacacg cacggagcgc tccaccctct ttctatacgg tgccacgtcc
     1501 ctgcccgaac gcgatatgca ctgcggagtc atcgagtgca acgatgagaa acatttcgaa
     1561 agaatcgtcc aacaggccga aaaggtattc gaacaaccat cgacccctgc catcatccga
     1621 ctcattgatt cgttctttgc cccccatttc ttctcggtcc gtgaactgag cgatgccggt
     1681 cgagccgaat tcatcgagca taccgtcacc gaccttctcg aacgattggc gaccacctac
     1741 ggctacttat acgatgaaca ccgaaaaacc atgcaaacgt tcgagcgcgt gggtgccgaa
     1801 gttcccgtcg aacttcgatt ggtcgcggag tacacgttgg cgcgacgatt ggatgcagcg
     1861 ctcctagaag caagcgatcc cacggatcca gggctcgagc gaacggttcg ggacattgct
     1921 catcaagcgc aagagcttgg cattcgattg gttgtgccgc gcagcaccct gcgtctgcga
     1981 caatggctcg atcaagccgc ggagcagtta caaaccgacg gatccacggc tcgggcgcaa
     2041 catgcctcgg ccttgctttc gctcacggag tctctgggaa ttcgcattcc catcgatcat
     2101 cttcaggacg cgatattcaa tcacctcaac catcgggtgg cttggccgga ggaattacaa
     2161 aagcgtctgg cgcagcaggt aggcgttcag caccccccgc attcaccaag ggacgaatcg
     2221 cacgagccga ctcacgggtt ggccaaaacc gaggcttagt gccgcaagtc aatcatcatt
     2281 ctgccaatcc ccctccaatt catcgagcaa ttcgttggcg cgatcctcgc tcaggccaat
     2341 ggcctccgca aacgtattga gcaattcgtt ttccgtgtcg tcgatgactt catctgcaaa
     2401 agccactgcc gcagccaaca cgaaagcgga ctcacatccc gcctcgtcgt caccaagacg
     2461 gtgcgcaatg ctctcgattc gcgcttgttg cccttctttt tccaacgctt gttggtaact
     2521 ctcgagcatg gcgttcaagg tcgtggtccg aatactgttt tcggtgagtt cacgaacggc
     2581 gccccgaatg acattgtatt cgtcttcacc cacggttcca tccgcggcca tcatcagaaa
     2641 catggtttcg acgatcggat cgataccttg cacagcggtt ctttcttccg gagggagcag
     2701 tcccgctttg gcaagcacgt cttgatcgct cgaaatgacg atactcgggc gcgcacccgt
     2761 ttgcttgaga cgatcacgaa ggcgggacac ggtggcaggg cgaagattga tcatgaaagc
     2821 actccactcg atggggttgg gttgaccaaa aagagactct gctatcttcg atagacgggt
     2881 cgcagagtac aatttgcgaa tcccatcgac aatgatcacg ttggtttcac ggcagaatct
     2941 ttcggcttgg cacggtttgc gatggacgac tcctccgaac atcgcccgct cctccgatag
     3001 agcacggccc catgacaccc cggcgagccg aaccaatgaa ttcaaccgat cgtcctgctc
     3061 gaaccgatcg tgtcgaaccg taacgcctct cccacgagct tacgatgcaa agcaacccat
     3121 cacaccgatt tcccgacgct catttgccat gaaaccgaca ctatcgcggg tcggcacaac
     3181 accgaaatcc aatgctcgcc tgaacataaa acggcccgtg ctgcgtcgtt accggacggc
     3241 agcgcgcacg gcaattgtgc agataatgcc cccccttcaa gttcgaaatc ttcgactcgt
     3301 gatagtcgaa actctgcacc cattcatcca cgttgcccgt gagatcaaag accccatagg
     3361 gcgatacgca ttggctcatg gatccggaag gcaacaaaaa tggatagttc ggatcgccgg
     3421 aggcacgatc gaccatatcg aagtactttt tcttggtctc ttccgattga ccgggaagct
     3481 gctgcgaaat ccacttcggc tccgtataac acgcgggccg tgatgcttgt ctcttcaaca
     3541 gaggatcgta ttccgtgcat cgaccattgg catcctggtc cttcggaaga caattggaat
     3601 aacaacggct gatattgcat ccggatcggg attcttccgt gtctgcggtc caaatgccac
     3661 gcgtccaacc cgtcggataa ggttggcgtt cgggcccttc gcaagccagg gtccattcct
     3721 gctcttcaca aagccgtttt ccagcagctt cacaagtttc ttgggcctcg taccaactgg
     3781 caagacgcgc tggagtcgca ccgggctgat tcggatattc atagcgatcc atgcaaaacg
     3841 ctagacgaat gaccgaagga caatccaatt gaacgatgga gcccggcacc gccgaatctc
     3901 gcccaaagcc aaggattcgt tcgaccgtgc atccattgat gacatcgccc gctttcgcac
     3961 ccagaaactt ctttccgaat gcatgtttcg tatccgcccg gtcttgatcg tgtttgtctc
     4021 caggaacgac tttgagatga acggatactt tttttcgtgc cgttccgcat ttcgacgacc
     4081 cgggcacata agcgatacat tggtcatcga accccagcgc tccgacagcc gttttatccg
     4141 ccaagctgtc ggtacattga tgcgtgaccg tcatgcaata gtcgccttcg acatacacca
     4201 ttccctcggg gcatcgaccg ctcgaatcgt cttgcttgtt ttgctcttcc accacgtcct
     4261 tcgcggcgcc atccaaggaa ggttttgcta cagcgtcccc agcgttgtca tcggcggcat
     4321 ccgcctgcct ccaaacatcc cgttgttcga aggcatctgc ttggttgacg atatcctccg
     4381 cggcatctct ttgcgcgcgc gctcgggggc cgtcagtcaa tccggccacg gcatccggat
     4441 ctgacatgcc atcatcacca tcgttgcagc aaatcgcgca acacgcggaa aagcagcagg
     4501 tggcgaccag tacgctctta gccactttga taccaagtgc agtgcgggga cgacgaggca
     4561 agtgcgaggt tgtcatgatg cttccgaaac gtggctcgaa ggtatcaggc ttgtcggaag
     4621 gaaccaaaaa cgcggcctga attcatgtaa ctaattgttt tataatagtt tttcaaatta
     4681 tcccgagcgc atggttccct cctttcatcc atggtggtgc gtcgacgaca agacaagtca
     4741 tggcggccac gttgcggttt cgaattgccc cttcttggcg tacaaccggg ccatgcatac
     4801 tgtgattgtt tccgcaccct tgcccggcac tgccgtcgca agactcacca ccgctggaca
     4861 tcgcgtcatt caaggggacc aacccggagg tctcggacac gaaggaatca tccgtgtact
     4921 acgcgaccac cccgatactt cggctctcat tcctctcgtt tccgaccgtg tagatgcacg
     4981 agttctcgat gcggggcctt cccttcgtat cgtggccaat tatggcgtcg gtatcgacaa
     5041 tctcgatctg gtggaattga agcgtcgagg aatcgtggcc accaatacac ctggcgtgct
     5101 gacggaagcg accgcggatt ttgcgatggc attgatattg gatgcttgtc gaaacgtaag
     5161 ccggggcgac cgcctggtac gcgcagggct ttggaaaggg tggtctccca cccagctcct
     5221 tggtccccgg gtgacgggat caaccctcgg gatcgttgga tttggccgaa tcggtcaggc
     5281 cgtagccgcg cgcgcccgcg ggttttccat gcgcgtcctt tactccgatc ccattcgtgt
     5341 cagtccccaa atcgagaagg atctacaagc cacctatctg tctctcggcg atttgttggc
     5401 gcaaagcgac atcgtctcgc ttcactgtcc ctccacggat agcacacggg gattgatgaa
     5461 tgagacgaga ctgcgacaga tgcgtcccgg ttccgtattg gtcaacacgg gtcgagggac
     5521 gtgtgtcgaa gaagctgccc tcgccaaatt gctgaaagaa ggccatcttg ctgcagccgg
     5581 tctcgacgtc tacgaacgcg aaccgatgat tcatcaagag ctgttggacc ttcccaatgt
     5641 ggtgctggct ccccatctcg gcagcgccga cctgcctgcc cgcgaaggaa tggcggagtt
     5701 gagtgtatca tccgtcatcg cagtccttgc gggaaacgaa ccagcccatc gcgtggcctg
     5761 aagtcccctc cctttctcgc cggtatccat ccgcatgtct ttgacgcggc aacgcatcaa
     5821 tgcggtctat cctgagggtt ggcaaaccct ggagcgagca tttgagtcca ccgctcgatg
     5881 ttctgaatgc cttccgattc catataggca gtcgtggctg ccaaggccgc ctcatcatga
     5941 ctccgtatcg ccagccatcg acgagccacg ttggcgtatt gacccatttc ggcagcttcg
     6001 aatccttcga tggccttccg caagcacgcc acacaacgct cggattgatt ttcgatggaa
     6061 gcgatcgctg cgcgaatgag ttccgccatg ggaggagcca cgatggagta ttcgcgggca
     6121 agctccgcgg catcggattt ggcgcgacgc aacagtttcg tggctctttc tggacattga
     6181 tgaacggctg ccagcgcgct tcgcgctcga agatgcagca tctcgatgcg cacggtttgc
     6241 acttgcagca gcatcgaagc gcgtaaggga gaccaggctt ggtcgacgcg cttcaacgcc
     6301 gactccccat cgccgatata caagtcgatc tgtccatatg cgtatagtgc ataataatgc
     6361 tgggcatgga aaccacgacg agaccagtgt tccatggctt catcgacagc gcgacgagct
     6421 tcttgcggtg tgtcgtccac gagccatgcc atattgctcg tgaacagccg tagatcggtt
     6481 gcggaataca agtctcctcg ttcaaaagca gcttcgagct gcgctttggt tcttttggcg
     6541 gccgctccga cgtgcccaat ccacatcagg ctccagatgg cataatgtcg aatggtcgtg
     6601 cgttcccagg acacgccgga acaatgggta aacgctgcca tagccttatc gccttccgag
     6661 atacaatctc caaatctgcc ttcgaagtag gctacggctg ccgcccccac atggtaccaa
     6721 ccaagcgtga acgggtcgcc aagcttgctc gtgagcgctc gggatgcgtc gagcaactcc
     6781 atgcatttgt catggcactt gggccctgcc accgcgagca tcccggcttg tagcgcaaga
     6841 ccacgcgcaa ttcgcgaagg ttctccagcc gaaagagcta gcaacaacgc acgaacctgc
     6901 aaatccgcac cgcgaatcgt atcgcaattg gaaagcccca ccgcaagcgc ccagcccaca
     6961 tcgatccgtc gcagtatctc cggatcatgg tgataggtgc cgcggaatcc aaccccccgt
     7021 acacgcagcc atgctcttcg ccacaatagg gataggagag cccccaacga tgttttcgta
     7081 agcttcatgc ccgcggccgc aagcatgtcg tcgatcagag ctagtccctc atcgaccttt
     7141 cccaccagca atgcttgttc cgcagccttt cgtcgtattt ccaacacgtc ttgcgcagga
     7201 gccgtaacca acgcttcgag ataagccgcc gaggcgtccg caccatggcc agcgttggcg
     7261 agggcgtcgg ccaatttgat tctcaattcg tacgtgcgac gctccggttt gagaagcagc
     7321 gcctctcgat acaggtccgc tgcccggcga aacgcaaggg cttcggcagc cctttccgcc
     7381 gccgccaccg cacacctcca ggccgactcg cgatctcccg aacctcgata gtgaaccaca
     7441 agggtttccg gatctgcacc ccgactttcc aaacaacgag ccaacgcttg atgcaacgac
     7501 acacgctctt caagactcat tctcgacgtc acgatctcgc gaatccgatc gtgataaggt
     7561 tcgaccaaat ccgtcgatcg cactccttgc gtccgtatca aacgctgcgt gcgcaacaca
     7621 tcgagcgctt ctcgcaactc accggacaac cccgccacct ctcgggcgac tgacaactcg
     7681 atggaacgtc cagccaaggc caccacgcga agcattcgcg cggccggcgc ggataaggtt
     7741 cccaaatact cccaaagcac atcatccaaa cgaacccccg tcggagggct tgaaccaccg
     7801 tcgagcctta aagcatgccg aacgaggtgc aaaacaaata gtggacatcc gcccgattca
     7861 ctcgccaacg caaacggcag cgcgctgtca tggctgcctt gttcccgaag aatgagcgac
     7921 gccaatcgct cacaagcgtc ttgcgaaaga ggcgaaacct ccacttcgaa gcatcggtag
     7981 gccgcgcgcg tttgagcatg aacaatctgt cgaagaatcg gactcgtctc cgtgtcttcc
     8041 gaacgatgac tcagaatgag aagcacccga ggagcatccg gtggctgcaa aagattggaa
     8101 aaaaacgctg cactatcctc atcaccccat tgcaaatcgt cgatccacaa caccaccggc
     8161 ttgcgccgtg acacttcgcc aaacaacgaa cgcaacgctg aaaatgccga ttttcttacc
     8221 tcatgatagt caagcccctc caactcctcg ccatctccca tggccgcaat caccggaatc
     8281 ctgcgcaata cagggaacat cctcgcgagc ggcaagatat ccttcggaag atacgtaacc
     8341 acctcatcgc aagaccgagc caacaaccac tgcgtcaacg cgtcgatgag agaatccaac
     8401 gccttgtacg ggacagactc acattcgaaa catcgtccac tgagcacacg agcccgcttc
     8461 gcacgacgca aatcatcgac gaaaaagcgg acgagcgagg ttttccccat gccggatcgc
     8521 ccatagcccc gaagcatcgt cccttttcct tgcacaacgt tttcaaaagc cttcgacatc
     8581 agttcgaggc tcgcctcacg cccaacgaga tactccgatg gctgataggt gattagagaa
     8641 gcaacatcct cacctcgaaa cgacgaaatc tcgctgtcct cgtcgtgatc cgaagccgcc
     8701 acaagcgccg cttgaatgct ttcgagtttg ggacgatcct gaggacgacg cgcgagcaac
     8761 tgcatgctca atcgggcgaa atgccgctga ttgggttcga gcaagggctc ggggtccacc
     8821 ggatccgcca tctgtttccg aatgagcatc gatacgggct tgccatcgaa cgggagacga
     8881 ccggtcagca cctcgtacag catgacgccc acggaatacc aatccgattc cgccccatga
     8941 acgagaccag ccgccgcttc cggtgccagg tatgcgggcg ttcccacaat ctccgtcgcc
     9001 gcttcttgct cccccttctc atcgatgtcc gccagaagac cgaaatccag aacaaccaca
     9061 cgcccgtttt ccgtcaccaa tacgttttgc ggcttcaaat cacgatggag ttttcccgct
     9121 cggtgcaacg cccctacccc ttcggtcaat tgcatgagcg ccgaacgcaa tcgcgtcgta
     9181 ttcaccgttc cccccgaccc caacacacca gacacggccg tttgataatc ctccgaatca
     9241 atcgtcggcg cgtcgttcgg ataggccgcc cccggccgaa cgtgatcgaa aaaactcacc
     9301 ccatggatga gttccatcgt gaaacaccat cgcgagtcaa aacagaatag gtcatgcaga
     9361 cgcaccaaat tcggatgaac gatgtccgcc agctcacgaa actcccgctt gaagcgaaac
     9421 aacgaatgcg catcgagacg ctcgagacac ttcaaagcca cacgactctg caactcccga
     9481 tcatgcacct catacacgct gccaaacgcc ccctcgccca gcttgcgacg tacctccaag
     9541 cgagatggca gcgatagttg atgctcttgc gacgaccgtg acatggctcg atgggtaagg
     9601 ccgcttcaag atgccataac cggctagaaa gagatatttg actttttcct caatgtctat
     9661 cctcgcgctc cctcattgga cagactcatg gcgtcatcgt tccctcacgg ctatttataa
     9721 ggtgaatcgc tcatcggagc gcgagatttc aaagcaaaaa cgtatagtaa cgccatcgtc
     9781 acttgctccg cggctgtggt cgacgcgccg atccgaa
//
LOCUS       DAOSOR010000167         9796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6915_length_9796_cov_3.468330, whole genome shotgun sequence.
ACCESSION   DAOSOR010000167 DAOSOR010000000
VERSION     DAOSOR010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9796
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6915_length_9796_cov_3.468330"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..407
                     /locus_tag="PK710_10800"
     CDS             108..407
                     /locus_tag="PK710_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10252.1"
                     /translation="MSNKERTWSDMVRRIERELIEQGPSATDDRKVRFLTAAYLRDIR
                     DLLRKQSGRVVAPPAGSAWDVQDMPHQEEDGDTRPFAFIEELRVGRPQGDDPDKK"
     gene            complement(598..1317)
                     /locus_tag="PK710_10805"
     CDS             complement(598..1317)
                     /locus_tag="PK710_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10253.1"
                     /translation="MQEQDRPKPVLPKPKRDSKPVLSPIAEIPLPGTEEREYEPPPIF
                     EPASFPTPTVESTPVGKPEPTPEPEPEPTPEPEPTPEPEPTPEPEPAPEPKPEPKPEP
                     KPEPKPEPKPSPEAPKKKLDREAILAKYRARSAADPKTAELEKADPRHSEARRLARLF
                     ASEIVLYNPEGVQTGRQGKNLYRMLKKDIDRCRQVFKTRVDDDVRLKFDYLYDEIVKQ
                     VAEGDESRLGAEMPEPEAPRD"
     gene            complement(1774..2757)
                     /locus_tag="PK710_10810"
     CDS             complement(1774..2757)
                     /locus_tag="PK710_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10254.1"
                     /translation="MSPSRLTAGLCMLGFLGCSDAGGQLFEPGSGNAGTGGASNTGGS
                     GAAANGGSGGFLVDLDGGGNEAGPANDCADETKYVYVIDDKNRMYKFDPMIASKAAFS
                     LVGTMGCKGGEGPNSMSVGRDGYAYVLYGTQNPYSGGYNCAGVLKVDIKTAQCAGPSS
                     FQCGSAGFQKFGMGFATDSASTSSESLYLSNSLQPGLGRVDFTTGSVIRVGDLPGAAE
                     FTGNAKGELWGFFPDVIPPAVIQVDKTNGNVLTTFPLSPLPPLGTGGYAYAFAYWGGS
                     FYIFYQVNDYDSSTNVWRLDASGNVEKYIPNTGLRIVGAGVSTCAPIEPPK"
     gene            2883..5339
                     /locus_tag="PK710_10815"
     CDS             2883..5339
                     /locus_tag="PK710_10815"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOT10255.1"
                     /translation="MSIDRTLRVNAVGAMVVWLLSACSGGPSDVVFQADAGVSDVSQE
                     ANAGAGGVSGSGGTGGASAAGAAGTAGVAGIAGEAGHGAAPEDGGPDVSFTDVANEKE
                     VGFEYDAPPCSTGCDGGVCINGACCPLADACGDECCDTGQVCSFQHCVVPGTICIDAT
                     DCETDEYCEYSLGDPPQVPDAGTCVGGLSPATGRCLPKPPQCDPGVELVPGEPITCLP
                     VCEHRPQPGSFAPIVKAHWNKKDIMMAPVVIQLDDDNCDGVVDERDIPEMVFSTFVGG
                     DYNHNGTLWAISIVDGQIVEKWSFKPTTDAIHPGRSIAAGNIDGNPGNEIVVCTDNGK
                     VRAVDADGTPLWISGAGGCDMPSLGDLDGDGMPEVVVEGRILNGVDGSLKATMTPGPV
                     GSNIVLSDMDGDGLLDIISPAGIWGGDGQRKAESKIAGTYVAIGDFDLDGIPEVASIN
                     KSNHTLSVWRYDPSEPSGAKVLRTGIDINGTWPNTCPVGSSGHTTGGGPPTIADFNGD
                     GVPDVAVAGGIGYAVIDGTKILDPLVPANQTNLWLGKTQDCSSAATGSSVFDFEGDGK
                     AEVVYSDEQYLRVYDGTNGDVLFQECNTTGTLFEYPLVADVDNDGHADLIVIANAYSG
                     ITCPKDGSKQRGLRVFGTNTGRWVRTRRVWNQHAYHVTNVEEDGTIPTQEAPNWTQQR
                     LNNFRQNVQPLGEFGAPDLIVQVLPPPCNTEYALYARVTNIGQASVPAGVVVGFYAND
                     PLAGGTLLGSAVTSHPLYPAENEIVSYPYPNPPPGLSNGTTPLYAVVDDGNPPHAWVE
                     CRTDNNTGGPASGKCLGGGS"
     gene            5406..6653
                     /locus_tag="PK710_10820"
     CDS             5406..6653
                     /locus_tag="PK710_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10256.1"
                     /translation="MAKAFTVSCPNCGAGLQTDPRNTLTHQVSCQYCGTLVTMVPAAA
                     RKRTALGVPPVPTGPNVVLLDKPEVPKGQAVGFAITIGLAALLPMVIGLFAVVANSGI
                     FSELNSRFNPNLFPLECETGREVVIRKRNETRTDAFVFASTACTIRIVDSKLSGPYIV
                     KEGTGVKIIVENSELRVSKAVFQGGNNSSIELRKGSKVYADDAVFDGAQHSKLTIVGS
                     VVESKGSIFKSESFGEVNLKEKSRLKAAKHVLAGANSKLTVDNSEVVLGSSLVREGTD
                     YFTVDASNGSTIQSEAIAIVGGSYGTVRLSDSKLTGMTGAVDMGRGTVSLLEGATISS
                     RQDAVRMGDYGKVEVGQKARIESGAVAIRVGGHSDVSINGGMIKGIKAAIMADDYTTV
                     RLRRATVTGVKDIKGRYSKVIED"
     gene            6743..7981
                     /locus_tag="PK710_10825"
     CDS             6743..7981
                     /locus_tag="PK710_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10257.1"
                     /translation="MARVSSNNCPNCGAALKLDSRLQTVHSVSCQYCGTVVTVVPAAA
                     RRRTELGELPKIPSGPHVVMLDEKQVSPRAFGCVMAFVVFGIMGFILVGTVGSKFFPY
                     LNSRFNPWLFPMNCSTGEQIVIWGRTETSNEPLINASTFCKIRIIDSHLTAPLLVKGD
                     TGVEIIAENSELRVARAVLDGKTNAKLELRKGSKVHTNEYVVIGDTWASVSLSASSIE
                     SKGGVVKGENGPKLRARDKSRVVAAKAVMVGPDAEVLLDDSEMLLATSVFEEGADHVK
                     LSVNNRSTIRSENIAVMGGSHSEISVSASTITGKNGAIDMKDHDKLTLSDAATIRSPR
                     DAVQFQNHATVDVGSGSTIEAGTSALRVGKDANIRISGTVGGNTSAISAEDYSKVVLR
                     RATVRGPISVTGSYSSVKQE"
     gene            8112..>9796
                     /gene="glgX"
                     /locus_tag="PK710_10830"
     CDS             8112..>9796
                     /gene="glgX"
                     /locus_tag="PK710_10830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HOT10258.1"
                     /translation="MVYSFQQRTQRRVRSGHPYPLGATRLNEGVNFALYSKHATAVAL
                     LLFDDPHGEPTDVIVLPSRTRHVWHGFVEGIGHGQWYGYRVAGPFDPAMGHRFNPHKV
                     LIDPYAKALSHKMRNEDGLLLGFDRFAQEADLTLDERDNVRLVPKAMVVDDVFDWQGD
                     EPLDVPFRDLVIYETHVRGFTRHDSSKVAHPGTYLGLIEKIPHLLELGVNAVELLPVH
                     EFATEDFLRDKGLMNYWGYNTVSFFAPESSYASSDRPGAAIDEFKTMVRALHRAGIEV
                     ILDVVYNHSAEGNELGPTLSLRGIDNRTYYCLTGSPEQPMRRYVNFSGCGNSLNLSDP
                     IVLRLVMDSLRYWVQQMHVDGFRFDLASVLGREGGRFDRSASFFDVVTQDPVLNRVKL
                     IAEPWDIETFEIGNFPIEWCEWNARFRDTMRRFGRGDSGVLSDVGYRLTGSSDLYGHD
                     GRTPYHSINFITCHDGFSLADLVSYNRKHNEANGEDNRDGNDANESWNCGVEGPTTDP
                     EVLSLRRRLVRNHLCHLIFSLGTPMLLGGDEVLRTQGGNNNAYCQDNEISWMN"
BASE COUNT         2091 a         2406 c         3063 g         2236 t
ORIGIN      
        1 acacgaagcg cagccaaccg agaagtcttt ccaagtgtct ttgcctcttg acgtccgcta
       61 gccaacaccg ttggatgaat ggaccttcca aaacgaggtg tgctggtatg agtaacaagg
      121 agcgaacgtg gagcgatatg gttcgtcgca tcgaaaggga actcatcgag caaggccctt
      181 ctgccaccga tgaccgcaag gttcgtttcc tgacggcggc atatctgcga gacattcgtg
      241 acttgttgcg caagcaatcc gggcgcgtgg tcgctccgcc cgcgggaagt gcttgggatg
      301 tgcaagatat gcctcaccag gaagaggatg gcgatacgag accatttgcg ttcatcgaag
      361 agcttcgtgt aggacgtcca caaggcgacg atccggacaa gaagtaagga gtgagccacg
      421 gaaggttcga tgggcacgct gcgtacggga tgaaagcgtc gcgcatcgcg atttctcatc
      481 cgtcattcat tctgaaaatt caaatagtta tggttatcgg gggactggaa acgtgctgtc
      541 caatgaggag ggggccgcgt tggcgatcct gggattcgaa tcgtagacga acaagcctta
      601 gtctctgggg gcttccggtt cgggcatttc cgcaccgagg cggctttcat ctccttctgc
      661 cacctgcttg acgatttcgt cgtacaaata gtcgaacttc aagcggacat catcatcgac
      721 gcgtgtcttg aagacttgac ggcaacggtc gatatccttt ttgagcatgc gataaaggtt
      781 ctttccctgt ctgccggttt gtacgccctc ggggttgtac aacacgatct cggaagcgaa
      841 gagtcgggcg agacgacggg cttcgctatg tcgaggatct gctttttcta gttcggccgt
      901 tttcgggtct gcggcgcttc gcgctcgata tttggcaagg atggcttctc gatcgagttt
      961 cttttttggg gcttcagggc tgggctttgg ttccggcttt ggttccggct ttggttccgg
     1021 ctttggttcc ggctttggtt ccggcgccgg ttcaggttct ggtgtcggtt caggttctgg
     1081 tgtcggttca ggttctggtg tcggttcagg ttcaggttcc ggcgtcggtt caggcttccc
     1141 aacgggggtt gactctacgg tgggcgttgg gaacgaagcc ggttcgaaaa taggcggtgg
     1201 ttcgtattct cgttcttcgg ttcctggcaa agggatttcc gcgattggtg atagaacggg
     1261 cttcgaatcg cgtttcggct ttgggagtac gggtttgggg cgatcttgtt cttgcactgg
     1321 gctttcaggc ggttctgatt ctgctggggg tgcgaaaaga tcgacgggag gttgcgatgg
     1381 gggtttgctt gaaacatcgg ggaacagccc gcccccggtg gtgggtggtg attggtctgg
     1441 tgtaaggtca ggctgttgta tctcagtctt tggcgctggc tgtggcgttg atcgcgggct
     1501 gtcgctttca gggggcgtgg gttcccgttt cagttccgcg ggagcttttg tcgttggacg
     1561 tcgacgaaat agtaagccga ggataacgac aagcaggacg agggcggttg cgacgatggc
     1621 gaggtttata gggttcgaga gaagtggcat tgtgatcggc tcgcacaggg tacgtggtac
     1681 gttggggcca atggacctgg agagtaccat accgcacctt gcggtttggg atcattttcc
     1741 gggtgcaaac acgggcgaga aggctgaaaa aggctatttg ggcggttcga tgggcgcgca
     1801 ggtagaaacg ccggcgccaa cgattcggag gccggtattc ggtatgtatt tttcgacgtt
     1861 gccactggca tcgagtcgcc agacgttggt ggaactgtcg tagtcattga cttggtagaa
     1921 gatatagaag gatcctcccc aataagcgaa ggcgtaggcg taaccaccgg tgccgagggg
     1981 cggcagggga gaaaggggga acgtggtcag gacgttgccg ttggttttgt cgacttgaat
     2041 gacggcggga gggataacat cagggaaaaa tccccatagt tcgcctttgg cattgccggt
     2101 gaattcggcc gctcccggaa ggtcaccgac tcggatgacc gagccggtgg tgaagtcgac
     2161 acgtcccaaa ccaggttgga gagagttgga caagtacaac gattcgctgg aagtcgaagc
     2221 tgaatcggtg gcaaatccca tcccgaattt ttgaaatccg gcagatccgc attggaatga
     2281 ggaaggacca gcacactgag cggttttgat atcgactttg agaacgcccg cgcagttgta
     2341 tccgcccgaa tagggatttt gcgtgccata cagaacgtaa gcgtagccat cgcggcctac
     2401 ggacatggaa ttggggcctt caccgccttt acaacccatg gtgcctacga gggaaaaggc
     2461 cgctttcgaa gcgatcatgg gatcgaattt gtacatgcgg ttcttgtcat cgatgacgta
     2521 gacgtacttg gtttcgtccg cgcaatcgtt ggcgggtccg gcttcattgc caccgccatc
     2581 gagatccacg aggaaacctc cggaaccgcc gttggcggcc gcaccagagc cgcccgtatt
     2641 cgaggctccg ccggtacccg catttcccga tcctggctcg aatagctgtc cccctgcgtc
     2701 ggagcagcca agaaatccaa gcatgcacag cccggccgtc agtctgctag ggctcatcgt
     2761 gtttcctcca tgtttcctat agcggtagca cgagcgcttc cgtgcttcga gaacgttttt
     2821 tgcggatctg gtcccgttca tggatggtct gtcccgaggc gaagatgtgc tacgatcatc
     2881 gaatgtcgat cgatcgtact ttgcgcgtga atgctgtcgg cgcgatggtg gtttggctcc
     2941 tcagcgcttg ttccggcggt cccagtgacg ttgtgtttca ggccgatgcg ggcgtgtcgg
     3001 acgtttccca agaggcaaac gcgggcgctg gaggagtgtc tggaagcggt ggaacgggag
     3061 gggcttccgc cgcaggtgcg gcggggaccg caggagttgc tggaattgca ggggaagcgg
     3121 gccacggggc ggcgccggaa gatggtggcc ccgacgtgtc tttcacggat gtagccaacg
     3181 aaaaagaagt gggattcgaa tatgacgcgc cgccttgttc gaccggttgc gacggcggcg
     3241 tatgcatcaa tggcgcctgt tgtcctttgg cggacgcctg tggtgacgaa tgctgtgaca
     3301 cgggtcaggt ctgttcgttt caacattgcg tggtccccgg caccatctgt atcgatgcga
     3361 cggactgcga aacggatgag tattgcgaat actcactggg tgaccccccc caagtaccgg
     3421 atgccggaac ctgcgtgggt ggcctttctc ccgcgacagg acgatgcctg cccaaaccgc
     3481 cccaatgcga ccctggcgtc gagttggttc caggcgaacc gatcacctgt cttcccgttt
     3541 gtgaacatcg tccgcaacca ggctcgtttg cccccatcgt caaagctcat tggaacaaaa
     3601 aagacatcat gatggctcct gtcgtcatcc aactcgatga cgataactgc gatggtgtcg
     3661 tggatgaacg tgacatcccc gagatggtgt tttccacgtt cgtcgggggc gattacaatc
     3721 acaacgggac cttgtgggcc atatcgatcg tggatggaca gatcgtcgaa aaatggtcgt
     3781 tcaagccaac caccgatgcc attcatccag gccgttcgat cgcggcggga aacattgacg
     3841 gcaacccagg caatgagatc gtcgtgtgta ccgacaatgg caaggtgcga gcggtcgacg
     3901 cggacggcac accgttgtgg ataagcggcg cgggcgggtg cgatatgccg agtctcggtg
     3961 atctggatgg agatggcatg ccggaagtgg tcgtggaggg acgcattctc aacggcgtgg
     4021 atggttcttt gaaagcgacg atgacgcctg gacctgtagg ttcgaatatc gtgctttcgg
     4081 atatggatgg ggatggcttg ttggacatca tctctccggc gggaatctgg ggtggagatg
     4141 gacaacgaaa ggccgagagc aaaatcgctg gaacctacgt ggcgattggc gacttcgatc
     4201 tcgatgggat ccccgaggtg gcgagcatca acaagtccaa ccacacgttg agcgtatggc
     4261 ggtacgaccc ttccgagccg tcgggcgcaa aagtattgcg caccggaatt gacatcaatg
     4321 gaacgtggcc caatacctgc ccggttggga gttccggtca taccaccggt ggtggccctc
     4381 ccacgattgc cgacttcaat ggagatggtg tgccggatgt cgcggtagct ggaggaattg
     4441 gttatgcggt catcgatggg accaagatcc ttgacccgtt ggttcccgcg aatcaaacga
     4501 atttatggct tggcaagacg caagattgct cttcagccgc aacgggtagc tccgtattcg
     4561 acttcgaggg tgatggaaag gcagaggtgg tttattccga cgaacaatac ttgcgtgtct
     4621 acgatggaac gaatggggat gttctctttc aggaatgcaa tacgaccggc acgctttttg
     4681 agtatccgtt ggtcgcggat gtggacaacg atgggcacgc ggatctcatc gtgatagcca
     4741 atgcgtattc ggggatcacg tgtccgaagg atggttcgaa acagcgtgga ttgcgtgtgt
     4801 ttggaactaa cacgggaaga tgggtgagaa cgcgtcgggt gtggaaccag cacgcttatc
     4861 acgttaccaa cgtcgaggaa gatggaacga tccccacgca ggaggcgccg aattggacgc
     4921 agcagcggct caacaatttc cggcaaaatg tgcagccact gggagaattc ggtgcgccgg
     4981 acctgatcgt gcaagtgctt cctccaccgt gcaatacgga gtatgccctg tatgcgcggg
     5041 tgacgaacat cggtcaggca tcggttcccg cgggtgtggt ggtgggattt tacgcgaacg
     5101 atccgctggc cggagggacc ctgttgggtt ccgcggtgac gtctcacccg ctgtacccgg
     5161 ctgaaaatga aattgtctcc tatccgtacc ccaatcctcc gcctggattg tccaacggaa
     5221 cgacgccctt gtatgcggtg gtcgatgatg ggaacccgcc gcacgcctgg gtggaatgcc
     5281 gtacggacaa caacactggc ggaccagcga gtggcaaatg tctgggtggg gggagctgat
     5341 ttttcgtaat aatcaataaa aacatatggt tagatccgtt ttgtctctcc gggtaggggt
     5401 gaataatggc caaagcattt acggtttctt gtcccaattg tggggcgggc cttcaaacgg
     5461 accctcgaaa cacactcaca catcaggtca gttgccaata ctgtggaacg ttggtgacga
     5521 tggtgccggc cgcggcccgg aaacgaaccg cgctgggggt gccgcccgtg ccgacgggac
     5581 ccaatgtcgt tttgctcgac aaaccagaag tccccaaggg acaggcggtg ggatttgcaa
     5641 ttacgatcgg ccttgcggcg ctgctgccga tggtgatcgg attgttcgca gtagtggcca
     5701 attcgggcat attttccgaa ctcaactctc gtttcaaccc gaatttattt cccctcgagt
     5761 gcgaaaccgg gcgtgaagtg gtcattcgaa agcgtaatga aacgcggacc gatgcattcg
     5821 tttttgcatc gacggcttgt acgatccgca tcgtcgacag caaactgagc gggccgtata
     5881 tcgtcaagga agggacaggg gtaaaaataa tcgtagaaaa tagcgagtta cgtgtttcga
     5941 aagccgtttt tcaaggtggg aacaattcca gcatcgagct tcgaaaaggc tcgaaagtgt
     6001 atgcggacga cgcggtcttc gatggcgccc aacacagcaa gctgaccatt gtaggaagcg
     6061 tggtggaatc caaaggcagc atcttcaagt cagaaagctt tggtgaagta aatctcaagg
     6121 aaaaaagtcg cctcaaagcg gccaaacacg tgctggcggg tgcgaattcc aagcttacgg
     6181 tcgacaatag cgaagtcgtt ctgggatcga gcttggtgcg tgaaggcact gactatttta
     6241 cggtcgacgc tagcaacgga agcacgatcc aaagcgaagc gatcgcgatc gttggcggat
     6301 cctatggcac ggttcgtctg tccgatagca aactcacagg catgacggga gctgtggata
     6361 tggggcgagg cacggtatct ttgctggagg gcgcgacgat atcttcgcgg caggatgccg
     6421 tgcgaatggg ggattacggc aaggtggagg ttggtcaaaa ggctcgaatc gagtctgggg
     6481 ctgttgcaat tcgagtcgga ggccacagcg atgtgtcgat caatggggga atgatcaagg
     6541 gaatcaaggc tgccatcatg gcggacgact acacgacagt gaggctccga cgagcaaccg
     6601 tgacgggggt caaggatatc aaaggacgat attccaaggt gatcgaggac tgaggttttt
     6661 caaagggggc ggcaagatcg tccatcggag catcgacgga agcgttggtc aaacctatgt
     6721 tgacgttttc gccggaggtc acatggctcg agtgtcttcg aacaattgtc cgaattgcgg
     6781 cgcggcactg aagctggact cacgacttca gaccgttcac agcgtatctt gccaatactg
     6841 cgggacggtc gtcacggttg ttcccgcggc agcccgtcgt cgcaccgagc ttggcgaact
     6901 cccgaagatt ccctccgggc cgcatgtcgt gatgttggat gaaaaacagg tttcgccccg
     6961 ggcctttggc tgcgtgatgg catttgttgt tttcgggatt atgggattca tcctcgtagg
     7021 tacggtgggg agtaagtttt tcccctattt gaactctcgt ttcaatccgt ggctctttcc
     7081 catgaattgc agcactggag agcagattgt gatctgggga cgaacggaaa ccagcaacga
     7141 gcctctcatc aacgcttcca cgttttgcaa gatacgcatc atcgacagcc acttgacggc
     7201 tccgctcctt gtgaaaggtg ataccggcgt ggaaataata gcggaaaata gcgagcttcg
     7261 cgtagccaga gcggtgctgg atggaaaaac aaacgcgaag ttggagcttc gcaagggttc
     7321 taaggtccat accaatgaat atgtggtgat cggagacacg tgggcgagcg tttccctttc
     7381 cgccagttcc atcgaatcaa aagggggagt ggtaaaggga gaaaacggtc cgaagcttcg
     7441 tgcccgggac aagagccgtg tggtggctgc caaagctgtc atggtgggtc ctgatgccga
     7501 ggtattgctc gatgacagtg agatgctctt ggcgacgagc gtcttcgagg aaggagctga
     7561 tcacgttaag ctttcggtca acaatcgaag tacgattcga agcgagaaca tcgctgtgat
     7621 gggaggcagc cacagcgaga tctccgtttc tgccagcacc atcacaggaa aaaacggggc
     7681 cattgatatg aaggaccatg acaaactgac gttgtccgat gccgcaacca ttcgatcgcc
     7741 ccgcgatgcg gttcaattcc aaaaccatgc gactgtggac gttgggtcag gctcaaccat
     7801 cgaggctggc acttcagccc ttcgagttgg caaagacgcc aacattcgca tatcggggac
     7861 ggtgggcggg aataccagcg cgatctcagc ggaggactat tctaaggtag ttctacgtcg
     7921 cgctaccgtt cgtggtccga taagcgttac gggaagctac tcttcggtca aacaagaatg
     7981 aaccgaatgg gggctcgagg ttggtgagcg catacgtttt ttttagagac ctttgcgcga
     8041 tgacctccgc gttatcctga ttcatgcggg gatgttgtcc ttcgctcaaa gatcgatcca
     8101 ggatcgttct catggtgtat tcgtttcaac agcgtacaca acgtcgcgtg cgttccggtc
     8161 atccctatcc gcttggggcc acccggctga acgaaggcgt gaatttcgcc ctatattcaa
     8221 agcacgccac agccgtcgcg ttgttgctct tcgacgatcc gcatggcgag ccaacggatg
     8281 tcatcgtact accgtcgcga acacgccacg tgtggcatgg tttcgtcgag gggataggcc
     8341 acggacagtg gtacggctat cgggtggcag gaccgtttga tccggccatg ggtcatcgtt
     8401 tcaatccgca caaggtcctc atcgatccgt atgccaaagc gctttctcac aagatgagaa
     8461 acgaggatgg attgttgctc ggcttcgatc ggttcgcgca ggaagcggat ctgacattgg
     8521 atgagagaga taatgttcgt ttggtgccga aagcgatggt ggtggacgac gttttcgact
     8581 ggcaagggga tgagccactc gatgtaccgt ttcgggactt ggtgatctac gagacccacg
     8641 tgaggggctt tacccggcac gattcgtcga aggttgccca tccgggcacg tacctgggtc
     8701 tcatcgaaaa gattccgcac ttgctcgagc ttggcgtgaa cgccgtggaa ttacttcccg
     8761 tgcatgagtt tgccacggag gattttcttc gcgacaaggg gctcatgaat tattggggat
     8821 acaacacggt gtcttttttc gcgccggagt cttcctatgc gtcgagcgat cggcctgggg
     8881 ctgccatcga tgaattcaag acgatggtgc gtgcgcttca tcgagcgggc atcgaagtga
     8941 ttctcgacgt cgtttacaat cattcggcgg aaggcaacga actcggaccg acattgtccc
     9001 tgcgaggcat cgacaaccgc acgtattatt gtctcaccgg cagccccgaa cagccgatga
     9061 ggcgatacgt caacttcagc ggttgcggta attcgctcaa tctcagtgat ccgatcgtcc
     9121 ttcgcctggt catggactcg cttcgctatt gggtccagca gatgcatgtg gatggttttc
     9181 gattcgacct ggcatccgtg cttgggcgtg aaggagggcg attcgatcga tccgcctcgt
     9241 tcttcgatgt ggtgacacag gatccggtgc tcaatcgagt caaactcatt gcggagccct
     9301 gggacatcga gacgttcgag attggaaact ttcctatcga atggtgcgaa tggaacgcga
     9361 gattccgcga taccatgcgt cgattcgggc gtggcgattc cggggtgttg tcagacgtgg
     9421 gctatcggct cacggggtcg agtgatttgt atgggcacga cggtcggacg ccgtatcaca
     9481 gtatcaattt cattacctgt catgatggtt tttcgcttgc ggatctcgtg tcgtacaatc
     9541 ggaaacacaa cgaagcgaac ggggaagaca accgggatgg caatgatgcc aacgagtcgt
     9601 ggaactgcgg tgtggaaggg ccaacgacgg atcccgaagt attgtctctt cgtcgacgct
     9661 tggtgagaaa ccacttgtgc cacctgattt tttcgttggg cacgccgatg ttgctggggg
     9721 gcgacgaggt gcttcgaacg caagggggaa acaataacgc gtactgtcag gacaacgaga
     9781 tcagttggat gaactg
//
LOCUS       DAOSOR010000168         9776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6939_length_9776_cov_4.043103, whole genome shotgun sequence.
ACCESSION   DAOSOR010000168 DAOSOR010000000
VERSION     DAOSOR010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9776
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6939_length_9776_cov_4.043103"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..1868
                     /locus_tag="PK710_10835"
     CDS             87..1868
                     /locus_tag="PK710_10835"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HOT10259.1"
                     /translation="MRPLHPLAPCISALFIFALGCGQSSGEPFSEVTEQRFDPIIGGV
                     KDTSSPANDAVVMLYITSDPSNPNAGKGACTGTLIAPNVVLTARHCVSETKPTVSCYN
                     DVISDYPANWIYILKGVEPDTIYTWGKQVFRDTSKSLCGHDLALIVTNSPMTTMIPKK
                     VRVKNGPSVGETFKAVGYGLTNPNDDYSAGVRYYRNGVKVTGFTWGTGDRDFDGTVSI
                     CQGDSGGPALSSSDAVFGVTSRGGGCYENNNVWTRTDVYKSLIDQAMAAANASYTGED
                     GTKYGSGGSGGTGGTGGSGGSGGSGGSGGTGGKGGSGGSGGSGGTGGKGGSGGSGGTG
                     GKGGSGGSGGYGGSGGSGGYGGSGGSGGYGGSGGSGGYGGSGGSGGSEGEGGNPSIAC
                     GPNLPCADGSPCVQSLGHYYCTQVCSAPDLSCPPYYTCVPNLGACFKSEQCDNDVCAP
                     GLVCVDNGGKYCTTYCDSAGGCPLGYACDAKWNVCFKSSSPGCGPYNDCGEGKICVDD
                     RGKYCTTYCDAAGACPAGYACAPDSGVCFRQRVSSYDDDSTTSSSCAMTGRTLSGSLP
                     WTLVGFAAGLIALRRRQRACATDLFSG"
     gene            complement(1832..3043)
                     /locus_tag="PK710_10840"
     CDS             complement(1832..3043)
                     /locus_tag="PK710_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10260.1"
                     /translation="MESYELCDFLRLSGDINLQLNARGVRHQNILGILLNDCVVPSED
                     NEILRDVLKYLDMAYAMERRKLGPLAVLHPLRAAAVLARVSGKPVVLDLLTSLLHDKL
                     EDIPFKNTTPQAAAEAEKYFHEMLEKIDPERQWYLMERLDWLTRKPTDTYYSYIGHLL
                     DHAVQAPSLLRVKLADRLDNTLDMRVDVRDPIDGVDFFAIVFQLLYVNGFRGYRPQTK
                     DHPDGSPMNGAKRLYQLFKNAVLMSLIRKRNSELNATARRLFTLLARASMKEAERIVL
                     HIFGYHINDLQMQRELLLDVMRYAQEGGLDRVTPPGAKHPLDGHFVSCFEDTNSKIRE
                     ENLNRLYQDKPMMVRSALAFIVIFMNFMEDDNYFVAGIHEGGMEADTEPASRRATALH
                     YPENKSVAQAR"
     gene            complement(3334..5253)
                     /locus_tag="PK710_10845"
     CDS             complement(3334..5253)
                     /locus_tag="PK710_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOT10261.1"
                     /translation="MRVKTLCLRDCPDSCGLVATVEQGQLLRVDGDPDHPITKGTICA
                     RTRRFPHHPSQSERLLFPLIRTNCGFRQASWEEAIGSVADTLLAIRAQSGPEAILHAR
                     FSGSMGVLAGMVDAFFSWFGPVTTLRGSVCNDAGKVAQEMDFGISDGHDVTDLDHSRH
                     ALLWGKNPFVSHLHLVPWLQNVRKRGGQVVLIDPVYHASARLADRVIQPRPGSDAFLA
                     CAVARKILDDGRAVDLGSMADNAESYEALLRSRPMEIWARQADVNDKDIAYLADLGMQ
                     RPCAFLLGWGMARRSQGGAIVRAIDALCALSGNVGIAGGGASYRIDGRGVFSGVKIPR
                     ASRTIPEPQFARAILEATSPAIRAVWITSGNPVVMLPHAKKVEQALRTRECCVVVDSF
                     LTDTTECATIVLPTTTMFEKEDMVHAYGHGWLRASVRAVEPPAGVLSDLEIVQRLARA
                     IDERSGGETNLAARLEGTARQWKERMATPLLETGIDMDEVERKAVRNPFAPRVPFDGG
                     VFPTTGGRMHLITEVPEEAQADPAHPLWLMSSSVRDAQGSQWSIDLPDVWPATCHPDA
                     APGWEDGDVVTLCNEQATMQVQLRFDERQRRDVMLVAKGGPLSKGQCANALVFDTKTD
                     LGEGAALLDAKVRIT"
     gene            5432..6592
                     /locus_tag="PK710_10850"
     CDS             5432..6592
                     /locus_tag="PK710_10850"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT10262.1"
                     /translation="MPRYLRRALLATMFFGASVGIFQATLNNYLADIHHLGAEARGWL
                     ELPRELPGFLLFAIAGALLMLVRETKMAAIAMSLTAIGALGLGFMASNTFTLVVFIIL
                     WSLGDHILFAVEGPIALRLAKGRREGHRLGQFGGARNLGTIAGVGLVFVCARLWGDRF
                     GLFYTFVALSAAMAGVLYASLRSDEGQPPSRKLVVKKKYSIFYAISALFGIRKQIFLA
                     FGAWVLVDLHRVSVETIALLYFIAASLGVVLRPLLGDVIDWVGERMVLAVDELLLLGV
                     CLTYAFASDLLPAPYALYGLYAAYVLDSVLFALRIARTTYLKKIADDPADITPTISMG
                     ITIDHLVAMSLPVVSGYLWEAYGYRWVFLLAGAIALAGFFVCLRIRTPAVES"
     gene            6940..8973
                     /locus_tag="PK710_10855"
     CDS             6940..8973
                     /locus_tag="PK710_10855"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOT10263.1"
                     /translation="MTHSRTKRRYGRGVAVASVAAAVWLTPFTASAIHFDPQGVLAFE
                     PDAILTESFESFPTGSGIEVVEGDALDGKRYVVLRGSSLDDAAYIPLSADFSQQAYQV
                     RAFVRGEHPWYPIVILQYGVGPGNSLDYITLSPTGRMTSDGWVEIESAPMSVDGKRLP
                     FAVLMVISEEIQLDALEVTPANVAYQPTTSCSKTKPCGVDQVCADGYCRGGNSMVPAL
                     PSKTHRNLVVDVLQNKLRIAFGGVETRQQPLQQALAKMEAMRGAESSWQFWSTFTDAI
                     HLLRDSHTTAIGLPSFVGFADRPFPICFVEGDADKSKTGAPSTPPYADVLVSHVGPYG
                     NLGLKPGDRLVSVDGMHPVAWLRSHPQAAWIGYQATDPEVFSGEVESLPRAIPALAST
                     IEVIRCNPSGTCAKPETIDLATLEGQAAGAQPECDHRPAYHLAANNPDPDTHDFDDVR
                     FGLLADSAPGEDLYGMIWNDTDNSSGHQYQWEKAYSEFRTKAKGLILDHRRGDGGTAP
                     GAAYLTELSRPPLTISVWSTIGTLGLFDLPFGEAEGKALFDLWKLDEDRAWKVGSTSP
                     REDMRIAVLLARDVSGSDFFPLGVKGSVNTRLFGRRTLGAFSTFFTLEAQGFYYWSQA
                     SGDFVTEDGKTHIAHGVEPDVVLVPKQSDLMVGVDTVYERALAWVRCGKEVCP"
     gene            8970..>9776
                     /locus_tag="PK710_10860"
     CDS             8970..>9776
                     /locus_tag="PK710_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10264.1"
                     /translation="MSRQRHSLLHTSAAIISCSLLLWACGAKTDLLSGSSAQSGGSGG
                     TSQQGGSGGTSQQGGSGGEQTGGSTSAGEGGGGAAGEAGTGGVAGGPIVRTILQRDPF
                     ENVDDPHNLLLDGGFEFSGSLSIPWNSGGYSSLRFGHGVTCRSGIRCAIFDKNEGIFA
                     IVVSPKSSIMNVTLHAKHDPPSCDGLSLYVLDVYNPTGASAFVAKTSTELDSDGWCEW
                     KGWAPAIPNAFPVLYIDTVHSRVVIDDVVVSLPEDEEQANAGLRALQQAPR"
BASE COUNT         1978 a         2644 c         2916 g         2238 t
ORIGIN      
        1 atttttcatt ggttgtaaga atgcaggcag cccaaaaata tcgtctcctc atggtcaatt
       61 ccacagtcgc ggagtagagt ttgaacatgc gcccactcca tcctctcgcc ccatgtatca
      121 gtgctctttt catcttcgcc ctgggctgcg gtcagtcctc gggcgagcct ttttccgaag
      181 tcaccgagca gcggttcgac cccatcatcg gtggcgtaaa agatacgagt tctcccgcca
      241 atgacgctgt ggtgatgctt tacatcacct cggatccaag caatcccaac gccggcaagg
      301 gagcctgtac cggaacgttg atcgctccga acgtggtgct cacggctcgt cattgcgttt
      361 cggaaacgaa gccgacggtc agttgctaca acgacgtcat ctcggactat ccggcgaatt
      421 ggatttacat tctcaaaggt gtggaaccgg acacgatata cacttggggc aagcaggttt
      481 ttcgcgatac gtcgaaaagc ttatgcggac acgatctcgc gctgatcgtg accaatagtc
      541 cgatgaccac gatgattccc aagaaggttc gcgtgaaaaa tggaccttcc gtgggggaga
      601 cattcaaagc ggtcggctat gggttgacca atccgaacga tgattattcc gcgggcgttc
      661 gctactaccg caacggcgtc aaagtaactg gatttacttg ggggacaggc gatcgggact
      721 ttgatggcac cgtgtcgatc tgccagggag actcgggggg accggcgttg tcgtcgagtg
      781 atgcggtgtt cggagtcact tcacgaggag gaggatgtta cgagaacaac aacgtgtgga
      841 cccgcacgga tgtgtataaa tctctgatcg atcaggccat ggcagcggcc aacgcttcgt
      901 atacggggga agacgggacc aagtacggtt cggggggaag cggaggaacg ggaggaacgg
      961 gcgggtcagg cggttctggt ggttcgggcg gcagtggagg gacgggaggc aagggtggtt
     1021 ctggcggttc tggtggcagc ggagggacgg gaggcaaggg tggttctggt ggcagcggag
     1081 ggacgggagg caagggtggt tcgggtggct cgggcggtta tgggggaagc ggcggctcgg
     1141 gcggttatgg gggaagtggc ggctcgggag ggtacggggg aagcggtggc tcgggagggt
     1201 acgggggaag cggtggctcg ggaggcagtg agggtgaagg gggcaaccct tcgattgcat
     1261 gtggtcccaa ccttccttgt gccgacggga gtccttgtgt ccaaagctta ggacactact
     1321 actgcaccca agtgtgcagc gcgccggatc tttcctgccc gccttattac acctgtgttc
     1381 cgaatcttgg cgcttgtttc aagtccgaac aatgtgacaa cgacgtttgt gcgcccggat
     1441 tggtttgtgt ggataacggc ggcaagtatt gcacgaccta ttgtgactct gcgggtggct
     1501 gtcccctcgg atacgcatgt gatgcgaagt ggaacgtttg cttcaaatca tcttccccgg
     1561 gatgcggccc ttataacgat tgtggcgaag gaaaaatatg tgtggacgat cgcgggaagt
     1621 actgcacgac gtactgtgac gcggcggggg cctgtccggc cggatatgcc tgcgcgccgg
     1681 acagcggcgt atgttttcga caaagagtca gcagctatga tgatgactcc acgacttcct
     1741 cttcgtgcgc catgaccgga agaacgttgt ctggatcgtt gccctggacc cttgtcgggt
     1801 ttgcggctgg actcattgcc ttgaggcgac gtcaacgcgc ttgcgcgaca gatttgtttt
     1861 cagggtagtg taaggccgtt gccctgcgag aagcgggctc cgtatccgct tccatcccgc
     1921 cttcatgaat acccgccaca aaataattat cgtcctccat gaaattcatg aagatgacga
     1981 tgaacgctag cgcgctccgt accatcatcg gtttgtcttg gtagagtcgg ttgagattct
     2041 cttcccggat ttttgaattg gtatcctcga aacacgacac gaaatggcca tcgagtggat
     2101 gtttcgctcc tgggggagtc acacgatcca atccaccctc ttgtgcatag cgcatgacat
     2161 cgagaagcaa ttctcgttgc atttgcagat cattgatgtg atagccaaaa atatgaagga
     2221 cgatcctctc cgcttctttc atggacgcgc gcgcaagtag cgtgaacaac cttctcgcgg
     2281 tggcattgag ttcgctgttc ctttttcgga tcaacgacat gagtaccgcg tttttgaaca
     2341 actgatacag acgcttcgcc ccattcattg gcgatccatc tggatgatct ttggtctgcg
     2401 gacggtagcc tcggaatccg ttgacgtaca gcaactgaaa cacgatggca aaaaagtcca
     2461 ccccgtcaat ggggtctctt acatccacgc gcatgtcgag ggtgttgtcc aaacgatccg
     2521 ccaacttgac gcgcagcaaa gagggcgctt gaaccgcatg atcgagcaag tggccgatat
     2581 acgagtaata cgtgtcggtt ggttttcgag tcagccagtc caaacgctcc atcaagtacc
     2641 actgacgttc cggatcgatt ttctccagca tctcgtggaa gtacttctct gcctccgctg
     2701 ccgcttgcgg tgtcgtgttc ttgaaaggga tgtcctctag cttgtcgtgc agcagggatg
     2761 tgagcagatc caacacgacc ggttttccgg atacacgagc cagtacagcc gcggctcgca
     2821 aagggtggag aaccgccaag gggcctagct tgcgacgttc catggcatac gccatgtcca
     2881 gatacttgag cacatcgcgc aagatctcgt tgtcttcgga agggactacg cagtcattga
     2941 gaagaatgcc gaggatgttt tgatgcctca cacctcgcgc attcagttga aggttgatat
     3001 ctcccgataa ccgcaaaaag tcacagagtt cgtatgattc catggatcac ctgaaaacgc
     3061 accgttgtga caggctcgaa caccacagac ttgacgatcg ggcgcaacgg tagcgcatcg
     3121 taacggtcta gcgcgtcttc gtttgcccgc aaggatatct tcgaaagtaa cttcctttgt
     3181 gacccgagca tccatggtca ttgaaattgc ttcaaccaag caggaattct atcccccatt
     3241 cgtcgcgaca attctgtgac agcaaaagaa acagaaacga tggatgagtc cagcccgtgg
     3301 tgaatgacgt tcatggcaat cgtcctgtca tcctcacgta atccgcactt tcgcatccaa
     3361 cagcgcggct ccttctccca agtccgtctt ggtatcgaat accagcgcat tcgcacactg
     3421 tcccttcgac aacgggccac ccttggccac gagcataacg tcgcgtcgct gccgctcgtc
     3481 gaatcgcaac tgcacctgca tggttgcttg ctcattgcac aatgtcacca cgtcaccgtc
     3541 ttcccatccc ggtgcggcat cgggatgaca ggtggccggc cagacatcgg gaagatcaat
     3601 cgaccactga gacccttgtg catcgcgaac cgagctggac atcagccata gcggatgagc
     3661 agggtcagct tgcgcctcct cgggtacttc ggtgatgagg tgcatgcgtc cacccgtcgt
     3721 cggaaacacg ccgccgtcga aaggaacgcg gggagcaaat ggattacgaa cggctttgcg
     3781 ctcgacttca tccatgtcga ttcctgtttc gagcaagggt gtggccatgc gttccttcca
     3841 ttgccttgcg gtgccttcca aacgcgctgc caggttcgtc tcaccaccgc tgcgttcatc
     3901 gatggcccgc gccaatcgct gcacgatctc gaggtcagac aaaacgccag cgggaggttc
     3961 gacggctcgt acggaggctc gcagccatcc gtggccgtaa gcgtggacca tgtcttcttt
     4021 ttcgaacatc gtggtggtgg gaagaacgat ggtggcgcat tcggtggtat cggtgagaaa
     4081 cgagtcgacg accacgcagc attcgcgggt tcgcagcgcc tgttccacct tctttgcgtg
     4141 gggcaacatg acgactggat tgcccgatgt aatccacacc gcgcggatgg caggcgatgt
     4201 ggcttcgagg atggctcgcg cgaattgggg ttcaggtatg gttcgagacg ctcgaggtat
     4261 cttgactccg gaaaacactc ctcgcccatc gatccgatag ctcgcgccgc cgcccgcgat
     4321 gcccacgttg ccactcaacg cgcacaacgc atcgatggca cgaacgatgg caccgccctg
     4381 gcttcgtcga gccatccccc aaccaagaag aaacgcgcaa ggacgctgca tcccgagatc
     4441 ggctaaatac gcgatatctt tatcatttac gtctgcttgc cgggcccata tttccatcgg
     4501 gcggctccga agcaacgcct cataggattc ggcgttatcc gccatggaac caagatcgac
     4561 ggctcgcccg tcgtcgagta tttttctcgc caccgcgcaa gcaaggaagg catccgagcc
     4621 tggacgcggt tgaatcacgc gatccgcaag acgagccgag gcatgatata cggggtcgat
     4681 gaggacgact tggccaccgc gttttcgtac gttttgcagc catggaacga gatgtagatg
     4741 cgatacgaaa gggtttttgc cccaaagtag cgcatgacga ctgtgatcga gatcggtcac
     4801 atcgtgtcca tcggaaatcc cgaaatccat ttcttgcgcg actttgccgg cgtcattgca
     4861 tacggaacct cgaagggtcg tgacaggccc aaaccaggag aaaaaagcgt cgaccatgcc
     4921 tgccaacaca cccatcgatc cggagaagcg agcgtgaagg atcgcttcgg gccccgattg
     4981 cgcgcgaatc gccaacaacg tatcggcaac actgccaatc gcttcctccc agcttgcctg
     5041 tcgaaaacca caattcgtgc ggatcaaagg gaatagcaag cgctcgctct ggctgggatg
     5101 gtgaggaaaa cgccgcgtgc gtgcgcagat cgtgcctttc gtaatggggt gatcgggatc
     5161 gccatcgaca cgaagcaact ggccttgctc gacggtagcc acaaggccgc aggagtcggg
     5221 gcaatcacga agacataggg ttttcacgcg catggggtcc ttggctccga aggggttggt
     5281 cgtgcatcca gcgaggctcg tggcaacggg cgcatgagga tggcttcgat gctcgagagc
     5341 gaaatcctac gctctttgct gtggaatccg aaagcgaatg ccatgcgcct ctaggcgaat
     5401 cctgtcggtg ggtctaacgt cgtcgcctgt catgccgcgg taccttcgac gcgcccttct
     5461 tgccacgatg tttttcggcg cctcggtggg cattttccaa gccacgctca acaattacct
     5521 ggcggatatt caccaccttg gagctgaagc gcggggatgg ctcgagttgc cacgtgaact
     5581 gcccggattc ttgctcttcg ccattgccgg cgccttattg atgctggttc gggaaaccaa
     5641 aatggccgcc atcgcgatgt cgctcacggc gataggggct cttggcctgg gatttatggc
     5701 ttcgaatacc ttcacgctcg tggtgttcat catcctttgg tccctgggtg atcatatttt
     5761 attcgccgtg gaaggaccga tcgcgcttcg tttggccaaa ggtagaaggg aaggacatcg
     5821 actcgggcaa ttcggcggcg cacgtaatct tggaacgatc gcgggcgtgg gtttggtttt
     5881 cgtgtgcgcg cgcctgtggg gagatcgctt tggtttgttc tacacgttcg tggcgctcag
     5941 tgcggccatg gcaggagtgc tctacgcttc ccttcgttcg gatgaggggc agccgccgtc
     6001 gcgcaaactc gtggtcaaga aaaaatacag catattttac gccatctcgg cccttttcgg
     6061 cattcgaaag caaatctttt tggcgttcgg agcctgggta ttggtcgacc tgcatcgtgt
     6121 gagtgtggag accatcgcgc tgctttactt cattgcggca agtcttgggg tggtgcttcg
     6181 acccttgctc ggggatgtga ttgactgggt gggtgagcga atggtgctgg cggtcgacga
     6241 gttgctgctg cttggcgttt gtctgacata cgcatttgcc agcgatttgc tgcctgctcc
     6301 ctatgccttg tatgggttgt atgccgcgta tgttctggac agcgtgctat ttgcgcttcg
     6361 cattgcgcga acgacgtatc tgaagaaaat cgccgacgat ccagcggata taacacccac
     6421 gatttccatg ggtattacca tcgatcatct ggtggccatg tctcttccgg tggtctctgg
     6481 atatctttgg gaagcgtacg gatacaggtg ggtatttttg ttggcgggag ccattgcgtt
     6541 ggctggcttt ttcgtttgtt taaggattcg cacgcctgcc gtcgaatcct gaccacgaat
     6601 catccaaaac ccatggttgt gccgtcgctc gtgatggcgg tggttgcagt gtggtttcgt
     6661 gggggtggac gatccgggaa gggcagcggt atgcctgcgc cggggtcgcg aatagtggcg
     6721 cttggtgagt gggtcgtgcc ttgttgtgcc gatggcttga cgctttttcg acggaatgct
     6781 ttcgcctttg tgctgcggta acgccgcgca ttcatttgtg ttattcggct aacggttctc
     6841 gcgtagtatc catcaattgc cgtcagtttt gttggcacag gcattgcggt gacaggagcg
     6901 ctcgcttccg agccgttttt cgctcgatgg aggtttctct tgacccactc acgaaccaaa
     6961 cgacgatatg gccgtggggt ggctgtcgct tcggtggcag cggccgtgtg gttgacgccg
     7021 tttaccgcct cggccattca cttcgaccca cagggagtgc ttgcgttcga gcctgatgca
     7081 atccttacgg aaagcttcga atcgtttccg acgggctccg gtatcgaagt ggtcgaaggc
     7141 gatgcgctcg acggcaagcg atatgtggtg ttgcgggggt cttcgctcga cgacgccgct
     7201 tatatcccgt tgtctgccga cttttcgcag caagcctatc aggtgcgtgc tttcgttcgt
     7261 ggggagcacc cctggtatcc gatcgtcatc cttcaatacg gtgtggggcc tggcaattcc
     7321 ctcgattaca taaccttatc gcccaccggc cgcatgacga gcgatgggtg ggtcgaaatc
     7381 gaatccgcac cgatgtccgt ggatggcaag cggcttccct ttgcggtgtt gatggtcatt
     7441 tcggaagaga ttcaattgga tgcgttggag gtgactcccg ccaacgttgc ttaccagccc
     7501 acgacctcgt gttcgaagac caaaccttgt ggtgtcgacc aggtctgtgc cgatgggtat
     7561 tgtcgagggg gcaattcgat ggtccccgcg ctgccgtcga aaacgcatag gaacctggtt
     7621 gtggatgtgc tgcaaaacaa attgcggatt gctttcggcg gcgtggagac acgacaacag
     7681 cctttgcagc aagcgctggc aaaaatggaa gcgatgcgcg gggccgaaag ctcctggcaa
     7741 ttctggagta cgttcacgga tgccattcat ctgttgcgtg actctcatac cactgccatt
     7801 gggttgcctt cgttcgtcgg attcgcggac cgaccctttc cgatatgttt cgtggaaggc
     7861 gatgcggata agtcaaaaac cggcgcccct tccacgccgc cttacgccga cgtgctcgtt
     7921 tcccacgtgg gtccgtatgg caacctcgga ttgaaaccgg gagacaggct ggtttccgtc
     7981 gacgggatgc atccggttgc atggttgaga agtcatccgc aagccgcgtg gatcggatat
     8041 caggccacgg accccgaggt attcagcggc gaagtcgaat cattgccgag agccatcccc
     8101 gcccttgcct ccacgatcga agtgatccga tgcaaccctt cagggacgtg tgcgaagccc
     8161 gaaaccatcg acctggccac tcttgaagga caggccgcgg gggcacagcc cgagtgcgac
     8221 catcgtcccg cctaccatct cgccgccaac aatccggatc cggatacaca tgattttgat
     8281 gacgttcgtt ttggattgct cgcggatagt gcgccgggag aagacctcta cgggatgata
     8341 tggaacgata cggacaatag ctccggacac caatatcaat gggaaaaagc ctattctgag
     8401 ttccgaacga aagccaaagg gctcatcttg gatcatcgtc gcggggatgg gggaaccgct
     8461 cctggcgcag cctatctgac ggaattgtct cgtccgccac taaccatatc cgtatggtct
     8521 accatcggga cgctcggact cttcgatctt ccttttggcg aagccgaggg gaaggccttg
     8581 ttcgatctct ggaaactcga tgaggacaga gcgtggaagg ttggctccac ctcaccccga
     8641 gaggatatgc ggatcgcggt tttacttgcc cgagacgtga gtggaagtga ttttttcccc
     8701 ttaggcgtca agggttccgt gaatacgcga ctgttcgggc gaaggaccct gggagcgttt
     8761 tcgacctttt ttacgctcga ggcgcaaggc ttttactatt ggtcgcaggc ttctggcgac
     8821 ttcgttaccg aagacggcaa gacgcatatc gctcatgggg tggagcctga tgtcgtgctg
     8881 gttcccaaac aatccgatct catggtcgga gtcgatacgg tgtatgagcg cgccttggcc
     8941 tgggtgcgtt gcggaaagga ggtgtgccca tgagccgcca acgacattct ctgcttcaca
     9001 cttcggccgc gattatttcc tgcagcttgt tgctttgggc atgcggtgca aagacggacc
     9061 tgctgtcggg cagttccgca cagagcggcg gctcgggcgg aacctcgcaa caaggcggct
     9121 cgggtggaac ctcgcaacaa ggtggctcgg gtggtgagca gaccggcgga tccacgagcg
     9181 ccggagaggg aggcggcgga gccgcgggcg aggcgggcac cggaggagtg gcgggaggcc
     9241 ccatcgttcg caccatcctg cagcgtgacc cgttcgaaaa cgtcgatgac cctcataacc
     9301 tgctgcttga tggcggattc gaattctccg gaagcctatc catcccttgg aattccgggg
     9361 gctattcctc ccttaggttt ggacatggag tcacctgccg ctcgggcatc cgctgcgcga
     9421 tcttcgacaa aaacgaaggc atattcgcca ttgtcgtatc gcccaagtcc agcatcatga
     9481 acgtgacgct ccacgcgaaa cacgatcctc cgagttgcga tgggctgagc ctgtatgttc
     9541 ttgacgttta caatccgacg ggagcttcag ctttcgtcgc caaaacctct accgaactcg
     9601 attccgatgg ttggtgtgaa tggaaaggat gggcaccggc gatccccaac gcattccccg
     9661 tattgtacat cgacaccgtt cactctcggg tcgtcatcga cgatgtggtt gtgtcgctgc
     9721 ctgaagatga agagcaggcc aacgcggggc tacgcgcgct acagcaagcg ccaagg
//
LOCUS       DAOSOR010000169         9737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6972_length_9737_cov_4.258108, whole genome shotgun sequence.
ACCESSION   DAOSOR010000169 DAOSOR010000000
VERSION     DAOSOR010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9737
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6972_length_9737_cov_4.258108"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..1492
                     /locus_tag="PK710_10865"
     CDS             71..1492
                     /locus_tag="PK710_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10265.1"
                     /translation="MTLGVKGLFEAGAHARWVDTFVIHQGLIGFRALCERVVPTDTRY
                     LVLDLDRTLHWDKNIGELLGWELCAHAAYGMAGLEQAEAERKSNRFVFDHKHPWRSVH
                     YVARGARLWAYPGLSYGLAIKLGMRSKAVRPWLFRMLGQDPVEVIHGIVRNALMHHLS
                     EIPLDTARTLARRVWHRQAADQILDKQDLLWLRERCPHIRIIVSSASPKPVLDVAAEE
                     LGVDEIYYTSVEEHDGYLSAPHALDRWFMLWRTPKRICPPSQAHTNAGERKMLRLLDV
                     HPDFLDPGVVTVGMTDTSYREDHSWANYFKILVDLHSPTPFVPIVSGSSPLMEIHSAH
                     VMTQAEKQDGSCRRRNHVGPLDERRYCGSQLERQLPGLLSTVHRLNQRYQDEHRRLHH
                     DRLEIEGHLSTLWKLIEEDVDDYNASEGHRRDRVLRRLRHRLRQRHRWERRLIRTERP
                     LAELTHSLTELLSVARLSLEPTA"
     gene            1655..4207
                     /locus_tag="PK710_10870"
     CDS             1655..4207
                     /locus_tag="PK710_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOT10266.1"
                     /translation="MALFPFSQRFFLFLVGVLTILISIVLFSTALDELLEGEAPAAVA
                     VQPPVLPALSRHVMVFCIDGLRWDLAQNPSVAPNYAKAMREHTHGAVMASPVTMTSSA
                     VRGIGVGLRSDLEQIVMNLNASAVKYNHLLDNAKAAGIRTALAGDITWAQILGDFDEQ
                     VVDANGIALETDNSDEILDAARRMVQSETPPHLLIIHLIAPDHFGHAYGTLDMRYREL
                     IRKIDGRMQAFFDEISPRWTVLAVSDHGQTDTGTHGMATDITRRTPLFAFGPGIRPGV
                     ELGEVDQVDLSATLAVLLGISGPARGRGTALVEMLDINEEQAASVVCADARRVIHVAR
                     EDGQESVAERLATLANGCGTNAPAVKRAAAARATVRAYDRAIDEARSGQGRTASLVAG
                     LSVLFLLLAGAWSCETNGNTRFYWRKRLGLTLTATMISAITVALTLYVERMAQPYDDV
                     VRGVLFAVAGVALLLIVGFPSFAADWFHRMSVVGWALVVGAVAWSYPTNTPHLAYGVL
                     LLFGLLWLLAPRADLAEGRVRTIVSGRFRLPWWRVVLFGLALVALLPAGYYHGDPTPP
                     WLRAHPAWMLGLGIVALVGWLLAGATMRDCHASKARILIDGVVAMAVASSSLLLRRVI
                     PADVGLAAMFVLPAGAVIALLARRNTLALGLLFAGYGWVSRDAEVLPLASAAIGLEAV
                     LHTVVRAKEPGDVAIEHRPWAVVLLATTLFAAVFLVRIGLQGGLDLTNIDYGVGGFGD
                     PEVSQFRVGAAMIWKYVSATWLLLTLVLVALKEQARMVIAAFMVSYAVRAATVAWMLF
                     VGRTSFWTATRTMSEIPSALIVSIVAALLLWVTLWRKKGISEFEAGSERARI"
     gene            complement(4283..4504)
                     /locus_tag="PK710_10875"
     CDS             complement(4283..4504)
                     /locus_tag="PK710_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10267.1"
                     /translation="MPILELRCCACGIVFHRPASAAQQDQVSWRLSTPMALMSPALEV
                     ETLRCPRCHDLRVEPVLRSFRPDPSRSST"
     gene            complement(4770..6053)
                     /locus_tag="PK710_10880"
     CDS             complement(4770..6053)
                     /locus_tag="PK710_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10268.1"
                     /translation="MNPHRIGSALGLSASAGLSALFLMGCPIYPSATECYDHEDCPRG
                     MYCNDDNQCVRPPSDGFGGSPSQGSGGSGGATNSTTHCTQPSDCRVNETCGQDGQCHP
                     GDCYFSGCVSGFECVLHGYQYVCLPSGSGGSSGSSGSSGSSGSSGSSGSSGSSGSAGS
                     SGSGGSGGAPIPCANPNDCDAGQVCSEKGLCEDGSCDDHGCVHGWICENDSCVPNDPH
                     HCIFDQDCIPFGIGFLCVNGSCTPPEDQCTDKTQCPDPASQSCVNGKCLSTCTSSSDC
                     PDGFLCDVTLGICSLPAVGCTITSDCTDPDTVCVAGACVSRCSSNDTCSEHFVCVANG
                     CVPDQNPSFLCDVDGTQDVCSAGSICLHHSCYITCTPNPDSCATHPPDLNQCKSTTSS
                     SGSYHVCGSSTNLGDECDPSSPCSGVKVCIDGFCR"
     gene            6397..8406
                     /locus_tag="PK710_10885"
     CDS             6397..8406
                     /locus_tag="PK710_10885"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOT10269.1"
                     /translation="MEVLCEHCQTEYDFDDALVSERGTTVKCTHCGHQFRIYRRPESA
                     SSMPERWEVRTSGGGDLVFTSLRDLQRAITRGQVGRSDVLTRGGLPSRPVSEIAELAQ
                     FFPEEGRVPRFTSDRPMAAPFESRPPIPSIHREDDESDFDEATVPKISAGTDRTLSTR
                     EVLGEEPGFEPATQRRPGLAETPAPPAVAVGGIKPLDQVEIREGRPLAMTPTPSDVRM
                     SYASTEEGFTDPRFVTTPARRSGAIRWVAGVVVVGALGILAATVGRSYFEKATEPTAG
                     ITVDPRIAGLLESGHEQLREGDWESAKASFDKASVLAENDPHVLLALAQLTNIRSDQT
                     WLKTRLIDPSEKEVLRITQSELDTRTKQAIAASSRVEAQDDPESIAVRVDAFRLAGEI
                     SKAKALAAKISPHAGKADIAYVLAALEMCENKPDWPVVIERLRTSVSSEHGLGRARAA
                     LIYALAQAGQAEAAQAELATLEKQTRPHALLLDLKAFVARVSKEGPVSSAKPTGPIDP
                     SHLPPMPGANRDGPDRAGDDKIAAGSYQDLLARGHAARQRGDLDSAEQFYRAALAKNP
                     GDTEALAGLGDVAKARGDKSASMGYYEEVNKQNPGYVPALIGLADAKWDAGDKAGAVA
                     LYQQIVSQTGGQGPYAARARQRIAEASKPTEDPKRTNTSTDAAPP"
     gene            8403..>9737
                     /locus_tag="PK710_10890"
     CDS             8403..>9737
                     /locus_tag="PK710_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOT10270.1"
                     /translation="MKRSRTIAFGLVAFFVGAFAMACQGRSRLEPVAAPSEIPSEFAD
                     EPTTPSRPHVAEINFYSGLPEIDRAGLLGTPKKRSFADLIELLTRIEQGKESKIKGLF
                     IIFGPQQGFARTQELARIVGRIRKGGMPVVCHAHDLGNETYWIAANACDRIWLSPAGS
                     LETVGIAAQVLYAKRLLAELRIDVDMLQVGKFKGAVEPFTHDGPSDEARASLMQMLTS
                     VRHQWLEGIEQARTGASPQMELGPYSPKEAKDKGLVDAVGMASEAREEAKTRANVSQA
                     VVRFGPGAKTVDTSGIAELVRALSGAGGATDEPHVAVLRATGAIAATSSGALFSDGDG
                     ISERQLTQSLFQVGKDEAVRAVVLRIDSPGGSALASDLLWHQLMQLRKIKPLIVSIGD
                     MAASGGYYLACAGTRVFAESSSIIGSIGVVGGKLAIGRGLDHLGVSAEVFPAS"
BASE COUNT         1912 a         2441 c         3230 g         2154 t
ORIGIN      
        1 acacaatgtt tacgtttgac agaagtattc tcgacgtaca gtccgcgcat cgagccagaa
       61 ggatgatgcg atgacattag gcgtgaaagg attgttcgag gccggggcgc atgcgcgttg
      121 ggtggatacc ttcgtgatcc atcaaggatt gattgggttt cgcgcgctat gtgagcgtgt
      181 cgttcccacc gatactcgct acttggtgct cgatctcgat cgaacgctgc attgggataa
      241 aaacatcggt gaactccttg ggtgggagtt atgcgcccat gcagcttatg ggatggcagg
      301 tctcgagcaa gccgaagccg agcgtaagtc caaccgtttc gtattcgacc acaagcaccc
      361 gtggcgttcc gttcattacg tggctcgagg cgcgaggctg tgggcctatc ctggtctgtc
      421 ctatggattg gccatcaagc ttggcatgcg ttcgaaggct gttcgaccat ggctttttcg
      481 catgttgggg caagatcccg tggaagtcat ccatggcatc gtacgcaacg ccctaatgca
      541 tcatctatcc gaaattccgt tggataccgc tcgtactctc gcgcgccggg tatggcatag
      601 gcaggccgcc gaccaaatcc tcgacaagca ggatctgctt tggttgcgcg agcgatgccc
      661 tcatatccgc atcatcgtca gcagcgcgtc acccaaaccc gtgctcgacg tcgctgccga
      721 agagcttggg gtcgatgaga tttactatac gtccgtggag gagcacgatg gctacctcag
      781 cgctccgcac gcgctggacc ggtggtttat gttgtggcgg acaccgaagc ggatttgccc
      841 gccgtcgcag gcgcatacca acgcggggga aaggaaaatg ctccgcttgc tcgacgtgca
      901 tccggatttt ctcgaccctg gggtggtgac ggtgggcatg acggatacca gttaccgtga
      961 ggaccatagc tgggcgaatt atttcaagat tctcgtggac ctccacagtc cgacgccttt
     1021 tgttcccatc gtatcgggtt cttctccttt gatggaaatc cactcggccc atgtgatgac
     1081 ccaagcggaa aagcaggatg gatcctgccg tcgtcgaaat catgtcggcc ccctcgatga
     1141 acgtcgctat tgcggttcgc aactcgagcg gcaattgccg ggactattga gcacagtcca
     1201 tcggctgaat caacgatacc aggacgagca taggcggcta catcacgatc ggctggagat
     1261 cgagggacat ttgtctacat tgtggaagct gatcgaagag gacgtggacg attacaatgc
     1321 ttccgagggg cataggcggg atcgcgtact tcgacgtttg cggcatcgct tgcgacaacg
     1381 tcaccgatgg gagcggcgat tgataagaac cgagcggccg ctcgccgaat tgacgcattc
     1441 gctgaccgag ttgctaagcg tggcgcgtct ttccctggag ccaacggcat gacagacgac
     1501 atgatcttcg gggctccagg cggtggcgga ttgcaggggc gcgttggcgc gggacatcga
     1561 tgtctcgctg ggcaggatgt cccgatttcg tggcggactt gaaggaggca agcccgcgtg
     1621 ttaggcgcgt cgttggcacg agagtgacaa aacgatggcc ctatttccct tttcccagcg
     1681 gtttttcctg tttctggtcg gtgttttaac gattctgatt tcgatcgtgc tgttttccac
     1741 ggcattggat gaactcctgg agggtgaggc acccgcggcg gtggccgtac aacccccggt
     1801 actacccgcg ttatctcgcc acgtgatggt tttttgcatc gatgggttgc gatgggattt
     1861 ggcccaaaac cccagcgtag cgcccaacta tgccaaggcc atgcgtgagc atacccatgg
     1921 cgcggtgatg gcgagcccgg tcacgatgac ctcgtcggcc gttcggggaa tcggcgtcgg
     1981 gcttcgcagt gacctggaac aaatcgtcat gaatctcaac gcttccgcgg tgaagtacaa
     2041 tcacttgctc gacaatgcga aagcggcggg gattcgaaca gcgctggcgg gggatatcac
     2101 ctgggcgcaa attcttgggg acttcgacga gcaagtcgtt gatgccaatg gcattgcgct
     2161 ggaaacggac aattcggacg agattctcga cgcggctcgt cgcatggttc agtccgagac
     2221 tccacctcat ttgctcatca tccatctcat tgcgccggat catttcgggc atgcgtatgg
     2281 gactttggac atgcgctatc gggaattgat ccggaagatc gatgggagga tgcaagcgtt
     2341 tttcgatgaa atttcaccac gttggaccgt tttggcggtg agtgatcatg ggcagacgga
     2401 cacgggcacc cacgggatgg cgacggacat cacgcgtcgg acgccgttat ttgcttttgg
     2461 gcctgggatt cgtcctgggg tggaattggg ggaagtggat caggtggatt tgagtgcgac
     2521 tctcgcggtg cttcttggca tttcgggccc tgcccggggc cggggaacgg cgctggtgga
     2581 gatgttggat atcaatgagg agcaggcggc atcggtggtg tgtgcggatg cgcgacgggt
     2641 catccatgtg gctcgtgagg atgggcaaga gtcggtcgcg gagcgactgg ctacgctggc
     2701 gaacggctgt ggcacgaacg ctcccgcggt gaagcgagca gccgcggccc gggccacggt
     2761 gcgggcctat gatcgagcga tcgacgaggc gcgatccggc caagggcgaa cggcgagcct
     2821 tgtggctggg ttgagcgtat tgtttttgtt gctcgcggga gcgtggtctt gcgaaacaaa
     2881 tggaaatacg cgtttttatt ggagaaaacg tcttggcctg acattgacgg ccacaatgat
     2941 atcggccatt acggtagcgt tgacgcttta tgtcgagcgc atggcgcagc cttacgatga
     3001 tgtcgttcgc ggggtgttat ttgcggtggc tggtgttgcg ctgttgttga tagtcggatt
     3061 tccatctttt gcggcggact ggttccacag gatgtcggtg gtgggctggg cgctcgtcgt
     3121 gggagcggtc gcgtggtctt atccaaccaa cacaccacac ttggcgtatg gcgtgttgct
     3181 gttgtttggc ttgctgtggc tgcttgctcc gcgcgcggat ttagccgaag ggcgtgtgag
     3241 aacgatcgtt tcgggtcggt ttcggctgcc atggtggaga gtagtgctgt tcgggcttgc
     3301 gttggtggcg ttgttgcccg cgggttatta tcatggcgat cccacgccgc cgtggctacg
     3361 ggctcatccc gcgtggatgc tcggattggg aatcgtcgcg ctggtggggt ggttgttggc
     3421 cggggccacg atgagggatt gtcatgcctc gaaggcgcga atcctcatcg atggggtcgt
     3481 tgccatggca gtggcgtcaa gcagcctgtt gttgcgccgg gtgatacccg cggatgtggg
     3541 gcttgcggcc atgttcgtgt tgcccgcggg ggctgtgatt gcgcttttgg cgagacgaaa
     3601 taccttggcc ttggggttat tgtttgcggg atacgggtgg gtttctcgtg atgcggaggt
     3661 attgcctttg gcatcggcgg cgatagggct tgaagcggtg ttgcatacgg tggtccgggc
     3721 gaaggagccg ggggatgtgg ccatcgagca tcggccatgg gcggtggtat tgctggccac
     3781 gacactattt gccgcggttt tcttggtgag aatcggtctg cagggggggc tggacttgac
     3841 caatatcgat tacggggtgg gcggttttgg cgatcccgag gtttcgcaat ttcgcgtggg
     3901 ggcggccatg atttggaaat acgtatcggc gacctggctg ctattgacgc tcgtattggt
     3961 agcgttgaag gagcaagcgc ggatggtgat tgccgcgttc atggtaagtt atgcagttcg
     4021 agcggccacc gtcgcttgga tgcttttcgt gggccgaact tcgttttgga cggcgacccg
     4081 aacgatgagt gagatcccca gtgctctcat cgtttcgatt gtggcagcgc tcctgctttg
     4141 ggtcacgtta tggcgaaaga aagggatttc ggagttcgag gcaggttccg agcgcgcaag
     4201 gatttgatgc cgcgacccga gcaaggatgg cgagcgctgg ggacgccacc atgggtagcg
     4261 cccaatcacg aggatgaggc tctcacgttg atgagcgaga aggatcgggt cgaaacgagc
     4321 gaagcacggg ttcgacgcgc aggtcgtggc accgtgggca tcggagggtc tcgacttcga
     4381 gggccgggga catcagggcc atgggagtgg atagtcgcca ggagacttgg tcttgttgcg
     4441 ccgcgctggc aggacgatga aaaacaatgc cacaggcgca gcaccgtaat tcgagaatcg
     4501 gcatgattcg gccccctttc caaggatgtg acgcttttga gtgtgacgca tccgggggca
     4561 acaatccagt catgatggca cgtcaagcca atccatcgga cgtcgcgcat gagacaacgc
     4621 cccccacgcc ctcgctatcc atttgcgtat agtcctcctg tgtttcagaa gtctacgaag
     4681 gtcgtgccat cgacatggtt tggatcatgg cggagggaga ctctccggta ggatatgtct
     4741 aagcgggaat cgtggcggaa tggatgagat catcggcaaa atccgtcgat gcacaccttg
     4801 acgcccgagc aaggggagga aggatcgcat tcgtcgccca ggttcgtgct cgagccacag
     4861 acgtggtagg aacctgagga cgacgtggtg cttttgcatt ggttgaggtc gggggggtgg
     4921 gtagcgcagc tatccggatt tggtgtacat gtaatataac agctatgatg aagacagatg
     4981 cttcccgcac tgcatacgtc ttgggtccca tcgacatcgc aaaggaagct ggggttttga
     5041 tcgggtacgc agccattggc cacgcaaacg aagtgttcgg agcaagtatc gttcgagctg
     5101 cagcgtgaca cgcatgcacc ggcgacgcac acggtatccg gatccgtaca atcactggtt
     5161 atcgtgcaac cgacagcggg aagagagcag atgccgaggg tgacgtcaca taggaatccg
     5221 tcggggcagt cggaggagga ggtacaagtg gaaaggcatt ttccgttgac acagctttgg
     5281 gatgcggggt cggggcactg agttttgtcc gtgcattgat cttcgggggg cgtgcaagaa
     5341 ccgttgacgc agaggaagcc tatcccaaac gggatacaat cctggtcgaa gatgcagtga
     5401 tgggggtcgt tcggaacaca gctatcgttt tcgcatatcc atccgtggac acagccatgg
     5461 tcgtcgcagc taccgtcttc acacaggcct ttttcggagc agacttggcc tgcatcacaa
     5521 tcgttggggt tggcgcaggg gatgggggca ccgccgctgc cgcctgagcc actgctgccg
     5581 gccgagccac tgctgccgct cgagccgctg ctgccgctcg agccgctgct gccgctcgaa
     5641 ccgctgctgc cgctcgaacc gccgctaccg ctaggtaaac agacgtattg gtatccatgc
     5701 aacacgcatt caaatcccga aacacatcct gaaaaatagc aatcgccggg gtggcattgg
     5761 ccgtcttgac cgcaggtttc gttcactcgg cagtcggaag gctgggtgca atgggttgtg
     5821 gagttggtcg cgccaccgga gccgccagag ccttgggacg gggagcctcc gaatccatcc
     5881 gagggaggac ggacgcattg attgtcatca ttgcagtaca tgcctctggg gcagtcttcg
     5941 tgatcgtagc attcggtggc gctgggatag atcgggcatc ccatcagaaa gagggcggac
     6001 agtcctgccg aggcggataa tcccaacgct gagccaattc ggtgtggatt catggtttct
     6061 tttccccggg cggtgattgt tttgagaagc ctagcaagcg ttatgccttg aacaaggctg
     6121 cattttcctg ggaaagggaa agagagaccg tgaaaatccg tttacaccct tggtgtgaaa
     6181 ctcatgggtc acggccgagc ggttcgaatc cgtcggatgg ccttgtttgg gcaaggcatt
     6241 ctttggtttc ggattgctgg atacgactgg gagcaacgtg ctacggtgcg gtcctcattt
     6301 ccgcgttggc gcgattcgtt ccgttttggt tcgccccaag cttctttggg tgctaggagc
     6361 ggggcgatgg catcaaaaat tggcctttga cgaaacatgg aagtactctg cgaacattgt
     6421 caaaccgagt acgatttcga cgatgctctc gtttcggagc gtgggacgac ggtgaagtgt
     6481 acccactgcg ggcatcaatt tcgtatctat cggcggcccg aatcggcgtc ttcgatgccg
     6541 gagcggtggg aagtgcgcac gagcggtggt ggggatttgg tattcacgtc tcttcgagac
     6601 cttcaacgag cgatcacgcg ggggcaagta ggtcgttcgg acgtgttgac gcgcggggga
     6661 ttgccatcgc gcccagtatc ggaaattgcc gaacttgcac agttttttcc ggaagaggga
     6721 cgcgtgcccc ggttcacgag cgaccgtccg atggcggctc cgttcgaatc tcgtccgccg
     6781 attccttcca ttcaccgaga ggacgacgaa agcgatttcg acgaggcgac ggttccaaaa
     6841 atttcggcgg ggacagatag aacgctttcg actcgtgagg tattgggcga ggagccagga
     6901 tttgaaccag ccacgcagcg gcgtcctggg ttggcggaaa cgcctgcgcc tcctgcggta
     6961 gccgtgggag ggatcaagcc gctggaccag gtggaaatcc gcgaagggcg gccgttggcg
     7021 atgacaccga cccccagcga tgtgcgaatg agttatgcat cgacggaaga agggtttacg
     7081 gatccccgat tcgtcacgac cccggctcgt cggtctgggg cgatccggtg ggtggcggga
     7141 gtcgtggtcg taggggcatt gggaatccta gcggccacgg taggtcggag ttatttcgag
     7201 aaagcgacgg agccgacggc cggcatcacg gtggatcctc gcatcgcggg gctactcgaa
     7261 tcgggtcacg agcagcttcg cgagggtgat tgggaatcgg ccaaggcgag ttttgacaag
     7321 gcgagtgtgc tggcggaaaa cgatccgcat gttttgcttg ctttggcgca gcttacgaac
     7381 attcgttccg atcaaacctg gttgaagacc cgattgattg atccctcgga aaaagaagtg
     7441 cttcgcatca cccaatcgga actcgatacc cgaacgaagc aagccatcgc ggcatcgagt
     7501 cgcgtggaag cccaagatga tccggaatcg attgcggttc gggtggatgc gtttcgactt
     7561 gcgggggaga tatccaaggc gaaagctctg gccgcgaaga tctcgccgca tgcgggcaaa
     7621 gccgacatcg cgtatgtgct agccgccttg gaaatgtgcg aaaataaacc agattggccc
     7681 gtggtgatcg agcgattgcg cacgtcggtc tcgagcgaac acggtcttgg cagggctcgg
     7741 gcggctctaa tttatgcatt ggcgcaagcc ggtcaggctg aagcggcaca agcggagttg
     7801 gccaccttgg agaagcagac aaggcctcat gctttgctgc ttgacctcaa agcgtttgtg
     7861 gctcgtgtgt caaaagaagg gccggtgtct tcggccaaac cgacgggtcc gatcgatcca
     7921 tctcatttgc cacccatgcc gggggcgaat cgagatggtc cggatcgtgc gggggatgac
     7981 aagattgcgg cagggagtta tcaagacttg ctagcgcggg ggcatgcggc gcggcagcga
     8041 ggtgatctcg acagcgcgga gcaattttac cgtgctgctc tcgccaaaaa ccccggtgat
     8101 acggaagcct tggcgggact gggcgatgtt gccaaagcgc gcggtgacaa gtcggcatcg
     8161 atgggatact acgaagaagt caacaagcaa aaccctggat atgtgccggc tctcatcggt
     8221 ttggcggacg cgaagtggga tgcgggggac aaggcggggg ctgttgctct ttaccagcag
     8281 atcgtctccc aaacgggtgg acagggtcct tatgccgcgc gagcgcgaca acgtattgcc
     8341 gaagcttcca agcccacgga agatccgaaa cgcacgaata cttccacgga cgctgctcct
     8401 ccatgaaacg gtcacggacg atagcctttg gcttggtcgc ctttttcgtg ggtgcctttg
     8461 ccatggcgtg tcaggggcgt tcccggctgg agcctgtggc cgctccttcc gagattccga
     8521 gcgaatttgc ggacgaaccg acgactcctt ctcgccccca tgtcgcagaa atcaattttt
     8581 attctggatt gccggaaatc gatcgagccg ggctgcttgg cacgccgaaa aagcgttctt
     8641 tcgctgatct aattgaattg cttacgcgga tcgaacaagg caaagaatcg aaaatcaaag
     8701 ggcttttcat cattttcggg ccacaacaag ggtttgctcg aacgcaagag cttgcgcgaa
     8761 ttgtcggtcg cattcgaaaa gggggcatgc cggtcgtatg ccacgcgcac gatttgggca
     8821 acgaaaccta ttggattgcg gcaaacgcgt gtgaccggat ctggctaagt cccgcgggct
     8881 ccttggagac cgtgggtatc gcggcgcaag tcttatatgc aaaacgttta ttggccgaac
     8941 ttcgcatcga tgtcgacatg ctccaggtgg gaaagttcaa gggggcggtg gagccattta
     9001 cccacgacgg tccaagcgac gaagcgaggg cttcgttgat gcagatgctc acctcggtgc
     9061 ggcatcaatg gctcgagggg attgagcagg cacgaacggg agcctcaccg cagatggaat
     9121 tggggccgta ttctcctaag gaggccaagg acaaagggct cgtggatgcg gtaggtatgg
     9181 cgagcgaagc gcgtgaggaa gcgaagacgc gcgcgaacgt ttcgcaggcg gttgtgcgtt
     9241 ttggtccagg agccaagaca gtggacacat cggggatagc ggagcttgtt cgggcgttga
     9301 gtggtgcggg gggagccacg gatgagccgc atgtggcggt actgcgtgcg acgggagcca
     9361 ttgcggcgac ttcttctgga gcgttatttt cggatgggga tggcatatcg gaacggcaat
     9421 tgacgcagtc gttgtttcag gtgggaaagg atgaagcggt tcgtgcggtg gtgttgcgga
     9481 tcgattcacc ggggggctcg gcgttggcga gcgatctgct ttggcatcaa ttgatgcagc
     9541 ttcggaagat caagccgctc attgtatcca ttggggatat ggccgcgagc ggcggctatt
     9601 atctggcgtg cgcaggcaca cgggtgtttg cggaatcctc gagcatcatc ggttcgatcg
     9661 gtgtggtggg aggaaagctt gcgattggtc ggggtttgga tcatctcggg gtatcggcag
     9721 aggtcttccc cgctagc
//
LOCUS       DAOSOR010000170         9720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_6983_length_9720_cov_4.089084, whole genome shotgun sequence.
ACCESSION   DAOSOR010000170 DAOSOR010000000
VERSION     DAOSOR010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9720
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6983_length_9720_cov_4.089084"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="PK710_10895"
     CDS             complement(<1..990)
                     /locus_tag="PK710_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10271.1"
                     /translation="MSPLRRRRTIRNLLKSLPISFCFVSWVSTHAIAQQPTTTEPRHS
                     SFLEVRLALSESASAQLAQRRLRRYLSIELEEAGHLAAQGQGPLSDHVAYVWIDFPDA
                     GNVTIQTRVGRGAVATRTFALREGLRPDVTARLVAIATSEMIRSQARPTRIRKPKEPK
                     PLSPEQVELATRQHPALLFTGKGQVVWLPSVAGTVGGSGMETAFRLGLWKPFLSGSWL
                     VGDSKGGPTRWVEAGLGADRSFFASPKLRWELGLGAYGATVHFRDGYFADGGRDTWAA
                     RVSGRFGAERVLTGSTWLGIAFEPAMLLRSAAFQASGTTGHIDGFSLGLGVFLQ"
     gene            complement(932..2206)
                     /locus_tag="PK710_10900"
     CDS             complement(932..2206)
                     /locus_tag="PK710_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10272.1"
                     /translation="MKQRGPCPISRCDLRDHATQERIDRIWERIEADLPMVESAAPAP
                     SRWIIGGIAAVAASLTAGLLLGKLIWDQPAAAIEMLDPPAVASTPRESSYEVLAAATR
                     GQTYALPGGGQAVLSPGSILELLRQRSDGVELRLVQGDVTIDAAGVPPERTFAVFAGE
                     SLLSTQGGGRVLVRRDDHGFEVQVSQGDARLQSPQGERKLVQGEQVRTAWMHTPRPVA
                     RHLSSPLHSARVMPTPTPVALQESAPPVSNVPDWLSRGRAGDSEEALRLLRAQPGGID
                     GAIMAASDADSLWEIHDIAIRQDPAAACRALIRVAEAFPGDASAQLAAFKLGNYYRRA
                     GQHELASKWFALAAQSPEGVLAEDALCKQFRTAPNETQALRFAQEYLVKYPDGRCKEE
                     AERLIAGEASDDTGESSTEATDNTKPAQEPSD"
     gene            complement(2203..2880)
                     /locus_tag="PK710_10905"
     CDS             complement(2203..2880)
                     /locus_tag="PK710_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT10273.1"
                     /translation="MASPAQHRPHLRLLPAEPEESPRSREQTPVSRSLRSYADLQDAE
                     LVALGIQGNRQALEWLYRRHAAFAIHLAIRIEGSAREADDIVHDSFVRAFERLREIRD
                     PGAFRSWLGSIVVREVRTHMRRARLMRAIGLIRAGDPIDLDAIASPDASPSTRAQIAQ
                     LYALLQTLPVDDRIAWILRSVEGHDHDTIVRMTSCSLATVKRRIDRAQSYLDEHFVDS
                     QASENLE"
     gene            3078..3278
                     /locus_tag="PK710_10910"
     CDS             3078..3278
                     /locus_tag="PK710_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10274.1"
                     /translation="MSKWAIFAILTSSLALACGGAPATPEVPEAPEAPAAETPEAPEA
                     PAGETPEAPAGETPEAPEAPTE"
     gene            3726..5201
                     /locus_tag="PK710_10915"
     CDS             3726..5201
                     /locus_tag="PK710_10915"
                     /inference="COORDINATES: protein motif:HMM:NF023327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3419 family protein"
                     /protein_id="HOT10275.1"
                     /translation="MSLPPSWIPASRRIFGASSLSDACLEYSDTNEDIDLTLKTLDVQ
                     PGHVVVGVTANADLLLSALRYDPAFVAGFDLNPAQNALAELKRTAIHSLDYHDYLVLL
                     GYRTHPRRRKIFESLLPSLPPLVRDFWTREDMTQAVNEGLWRQGTATRKDLEQWQRHL
                     LQLGRFLGPQTMNVVLGLAGSRHDREQIRSHVAQVFPRYAHSLHHPHRPSPFNPFSFP
                     NGIINYPACSKYAWGLVPEEHIYPPLSRKDFELIRPNLHRACFITSSMLDALSTMPNN
                     AFHRMYLSNITEYFSISEERALVEALLDKACFGAVVVLLFIALSPGQRRDLLDHDSWS
                     DRGWNRLFFGTRRWSDMHNDFKDFVVRGSDFFPRWARELRQKQSRERIVTQAKNRLVD
                     SWRRFSTADYAEAGRSSLSSAHFLLKHAQDRWEEASQLSHPDYVLIENEARTAAFYSD
                     LFANLRASHAHRSFYPFDYAILHKVRRHGATYELPELTTSV"
     gene            5308..5808
                     /gene="tpx"
                     /locus_tag="PK710_10920"
     CDS             5308..5808
                     /gene="tpx"
                     /locus_tag="PK710_10920"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOT10276.1"
                     /translation="MSKVTFHGNPATLEGKFPSPGDKAPPFTLVNGKLENMDLTSFSG
                     KKKVLTINPSYDTPVCAKAAREFNTRAATINDAVVLAISADLPFAQGRFCTAEGIEGV
                     VPLSAFRSPTFAKDYGVLITDGPLAGLTARAVVVIDENDKVIYSELVGEIVNEPNYEA
                     ALASLR"
     gene            complement(5833..7275)
                     /locus_tag="PK710_10925"
     CDS             complement(5833..7275)
                     /locus_tag="PK710_10925"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOT10277.1"
                     /translation="MNPWVDELRALLDEPLNDAAPTAFDPRILSLPMEPMLESARRFG
                     TPQYFLEADQLRSIAAHFVQTMQTSVPRSHAFYALKSNDLPALGQIVHQQGLQADVAG
                     LFELQLALELGFGTILFSSPGKTREELELALQHPNRVILIVDNLSELKRIIDLARAMA
                     PPRPLRIGFRLCAAPSEDPSWSKFGIPIEQLRYAIDQAQTCPNLTWCGLHVHSSWNKT
                     PEKYINNIETIAAWLADQPSSVRQRMSFLDLGGGFYPEGQAVLHKAEDKGILLQTLAS
                     RLGSSMTSAKPAFDPYACTITAVEPLHVFAQRIGDSVRRRLEPIIPNIQIFFEPGRYI
                     ASHPTHIVLSVLAVKPGGAIVDGGSQMLGDDRFFEYCFAPIVNVTRPSRERRRFTLFG
                     PLCDPHDVWGYTLWGQSPEEGDVLAVVGQGAYSFSSAWRFIKAIPPYVVWDNGKLWQA
                     KRAETFRNRYAGCELPCGGTLAGPKGSPEG"
     gene            complement(7272..8558)
                     /locus_tag="PK710_10930"
     CDS             complement(7272..8558)
                     /locus_tag="PK710_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10278.1"
                     /translation="MESFILFPAYGIGAIELRCCIESLHQRHQLDRLLVVAPPTLLEP
                     LPPLHGIADDLSDAKRVVEKTDAWAHAHQAQFEAVVGIDEELHFSLSRTLATHFGLPF
                     PSARTCALASNKWLCKQAFVEHGVPTSAFTLLSEADLDAAATIGYPNVLKVLSGTQSQ
                     FLFRNDSPKDLIRNISRLKKATLNTFSDPRFEPQPLSDSGQFLYPKHQFLLESFVGGI
                     EFSCDFLVQADHIALLRITRKLPGPTLGLFGGYLLVPPPCLSHHGIDEETLISVCHRI
                     VRAFGIHHGVCMVDFKVDRGSIHVLESSIRPGFSAFNHLMAKLLGYTSLAVMIAAAMN
                     EPIPLGYPSSEGAVLYLIAPPGHENSPIDLSGLHERTHDLGIIATHVYDTEGTASDVD
                     STPLIRGYVLVQPSDSSGIDKLMNILPQIVRYETRP"
     gene            complement(8648..9325)
                     /locus_tag="PK710_10935"
     CDS             complement(8648..9325)
                     /locus_tag="PK710_10935"
                     /inference="COORDINATES: protein motif:HMM:NF022216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOT10279.1"
                     /translation="MEVPALAALRPAIVRRLLPAPPCVSYTIGMARESRPPGFDMTYR
                     PQGRESFGPPLRTWILPTLYLVLAIAFVGLVMAASLHTSDSWLFRYIVEGDSHRILGA
                     RTLAVLVLLGGIAAMLRTSMRGVVIHPDGVEYRDVFGLSWPKIKNLSWAEIDEIVFES
                     SNVALRSWEGERIWLPAVLDRQGLQRALERVAVARGIPMQGKSLRPIDEIEAELDEMA
                     GGHERAE"
     gene            complement(9504..>9720)
                     /locus_tag="PK710_10940"
     CDS             complement(9504..>9720)
                     /locus_tag="PK710_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10280.1"
                     /translation="WSYRGTGSPLQGVVQAEGTGNDSTTHLCGVDSQGAVFCRGNNQH
                     GQCGNHPGIEYTANPVLVNLSGSPKEP"
BASE COUNT         2207 a         2767 c         2746 g         2000 t
ORIGIN      
        1 ttgcagaaaa acacctaaac cgagggaaaa accatcgatg tggccggtgg tccccgatgc
       61 ctgaaatgct gcggaacgca acaacatggc aggctcgaaa gcgatgccta gccatgtcga
      121 cccggtcaac acacgctccg caccgaatcg gcccgaaact cgagcagccc aggtgtcccg
      181 tcctccatcc gcgaaataac catctcggaa gtgaaccgtg gcaccataag ctcccagtcc
      241 aagctcccag cgcagcttcg gagaagcaaa aaacgagcgg tccgcgccca atcccgcctc
      301 gacccaacgc gtaggtccgc ctttcgaatc acccacgagc cacgagcccg acagaaaagg
      361 cttccacaaa cccaaacgga aagccgtttc catccctgac ccaccgaccg ttcccgccac
      421 cgacggcaac caaacgacct gtcctttgcc ggtaaataac aaagcaggat gctggcgagt
      481 ggctagctcg acttgctcgg gagacagggg tttaggttct ttgggttttc gaattcgcgt
      541 gggacgagct tgcgaacgaa tcatttccga ggtggctatc gccacgagcc gcgcggttac
      601 gtcgggccgt aacccttccc gcaaggcaaa cgtccgcgtg gccacagctc cccgcccaac
      661 gcgtgtctga atcgtcacgt tgcccgcgtc cggaaagtct atccacacat aggccacatg
      721 gtcactgagc ggtccttgtc cttgggccgc cagatgcccc gcttcctcta attcaatcga
      781 caaataacgt ctcaaccgac gctgcgcgag ctgggccgat gcggattcgg acagggcaag
      841 acgtacttcg agaaacgaag agtgtctcgg ttcggtagtc gttggctgct gcgcgatggc
      901 atgggtcgaa acccacgata cgaaacaaaa actaatcgga aggctcttga gcaggtttcg
      961 tattgtccgt cgcctccgta gaggactcac cggtgtcgtc ggatgcctct ccagcgataa
     1021 gtcgttccgc ttcttcttta catcggccat ccgggtactt gaccaaatac tcctgcgcaa
     1081 agcgcagtgc ttgcgtttcg tttggtgcgg tacggaattg cttgcaaagc gcatcctccg
     1141 caagcactcc ctcggggctt tgggccgcca gggcaaacca cttgctggca agctcgtgct
     1201 gtccagcgcg acgataataa ttgcctagtt tgaaagcggc aagttgtgcg gacgcatccc
     1261 caggaaaagc ctcggcgact cgaatcaacg cacgacaagc cgcggcggga tcctgccgga
     1321 tggcgatatc atggatttcc caaagagaat ccgcgtcgga agccgccatg atcgctccgt
     1381 cgatgcctcc tggctgtgct cgaagcaatc gaagcgcttc ctcactgtca ccagctcgac
     1441 cgcgagacaa ccaatccggc acgttggaca ccggaggagc cgattcctgc aaggcaacag
     1501 gcgttggtgt cggcatgact cgcgcggaat gcaggggtga ggaaagatga cgagctacag
     1561 gccgaggcgt atgcatccaa gccgttcgga cttgttctcc ttgcacaagc ttacgctcgc
     1621 cctgcggtga ttgaagccgc gcatcccctt gcgaaacctg cacttcgaaa ccgtggtcgt
     1681 cgcgacgcac gagcacccgt cctcccccct gggtggacag cagcgactcg cctgcaaaca
     1741 ccgcaaacgt tctttccggg ggaactcccg ccgcatcgat ggtcacgtcc ccttgaacaa
     1801 gccgtagttc aacaccatcg ctgcgctgac gaagcagctc aaggatggag cctggagaca
     1861 acaccgcttg cccaccaccc ggtaacgcgt aggtctgtcc acgagtagcc gcggccaaaa
     1921 cctcataaga cgactcccgc ggtgtggaag cgacagcggg cggatccaac atctcgatcg
     1981 cggcagccgg ttggtcccaa atgagcttgc cgagcagcag ccccgccgta agcgaagcgg
     2041 ctacagcggc aattccaccg ataatccaac gagaaggggc aggcgcagcc gactccacca
     2101 tgggcaaatc cgcttcgata cgctcccaaa tgcggtcgat gcgctcttgg gttgcatggt
     2161 cacgcaggtc gcaacgagaa attggacaag gtcctcgctg tttcattcca aattctccga
     2221 cgcttgggaa tcgacgaaat gctcgtcaag atagctttga gcacgatcta tccgccgttt
     2281 gaccgtggcc aaggaacagg atgtcatccg cacgatggta tcgtgatcgt ggccttcgac
     2341 cgagcgaagg atccaggcga tgcggtcgtc gaccggcaaa gtttgaagca gagcgtaaag
     2401 ctgagcaatt tgcgcacgtg tggagggtga ggcatcggga ctcgcaatcg catccaaatc
     2461 gatggggtct ccggcacgaa taagcccaat ggcacgcatc aaacgcgcac ggcgcatatg
     2521 ggtgcgcacc tctcgtacca caatcgaacc gagccagctt cgaaaggcac ccggatctcg
     2581 aatctcccgt aacctttcga aagctctgac aaaagagtca tgaacgatat cgtcggcctc
     2641 tctggccgat ccttcgatcc gaatagccag atggatggca aacgccgcat gacgacgata
     2701 cagccactcg agggcttgcc gattgccctg aattcccaga gcaaccagtt cggcgtcttg
     2761 caggtccgca taggaacgca aagaacggga aacgggcgtt tgctcccgcg accgggggct
     2821 ttcttccggt tccgctggaa gaagccgtag gtgcgggcgg tgctgggcag gtgaggccat
     2881 ggacagtcct taatgattat ggacgctcga ggagggaaaa gtagctcacg gggatgtttt
     2941 cttttcaagg gtgtgtcgat ggaggaagaa aaaacaaaaa acttgatcca ccgtgagcca
     3001 ttttgggaag cttgtgcgtc tggtaggaga gccgtcgata acttaccaaa cggctcattc
     3061 caacgttgga ggaaaacatg agcaagtggg caatttttgc aattttgact tcttctcttg
     3121 cgcttgcctg tggcggcgcc cccgcgactc ctgaggttcc tgaggctccg gaagccccgg
     3181 ctgccgagac cccggaagcc ccggaagccc cggctggcga aactccggaa gccccggctg
     3241 gcgaaacccc ggaagccccg gaagccccga ccgagtaatt gccttttcgt tttacgacga
     3301 cttgacacgg tggtcctttg ggtccgccgt gtttcgtttt gtgcgccgca gaaagctgcg
     3361 cacaaaacaa aaaaaacgcg cgagggagct gggggggcag catcaacctc gcgcggcagc
     3421 atgcgcaaga cggggtggag ggggggggcg gaatcacccc gccttgcctg ctgtcggtca
     3481 acttagcact cttcgtgcca taagcaacca taaaaaatca ataggttagt cattggtggg
     3541 acaacgttgc cttgcaaaca tgaaatatta attgcagttt cgatgaattg ctgtatttca
     3601 agataattaa tttataggtt tttcttccga aaggacgtgt tcaaccaaga gcaaacgctc
     3661 cctcgtttca tcgcttcatt gaccttcagc tcgtttcttc ccttcctttg ccccacactt
     3721 tgcccatgtc cctgccccca tcctggattc ccgcctcacg acgcatcttc ggtgcttcat
     3781 ccctgtctga cgcttgcctc gaatactccg ataccaacga ggatatcgac cttaccctca
     3841 agactctcga cgtccaacca ggccatgtcg tcgttggtgt cacggctaac gccgacctcc
     3901 tgctttccgc cctacgttac gatccagctt tcgtcgccgg tttcgacctc aaccccgcac
     3961 aaaacgcctt ggccgaactc aaacgaaccg ccatccactc tttagactat cacgattacc
     4021 tcgtcttact cggctaccgc acccaccccc gccgacgaaa aatcttcgaa agcctccttc
     4081 ccagtctgcc gcctttggtc cgtgatttct ggacccgcga agatatgacc caagcagtca
     4141 atgaaggact ttggcgccaa ggtaccgcca cacgaaaaga cctggaacag tggcaacgac
     4201 acttactgca actcggccgt tttctcggcc cccaaaccat gaatgtcgta ctcggtctcg
     4261 ccggctccag gcatgaccga gaacaaattc gctcccacgt cgcccaggtc ttccctcgct
     4321 acgctcacag cctccaccac ccccatcgcc cctcgccctt caacccgttc tctttcccca
     4381 acggaatcat caactatccc gcctgctcca aatacgcctg gggtctcgtc cccgaagaac
     4441 atatctatcc ccccctatcg cgaaaagact tcgaactcat tcgccccaat ctacatcgcg
     4501 catgcttcat cacctcttcg atgctcgatg ctctttccac catgcccaat aacgcgttcc
     4561 atagaatgta tctatctaat attacagagt atttttcaat ttcggaagaa cgcgctctcg
     4621 tcgaggcctt gctcgacaaa gcctgcttcg gcgccgtggt tgtcctgctt ttcatcgccc
     4681 tctcccctgg acaacgacga gatctgctcg atcacgattc atggtccgat cgggggtgga
     4741 atcgcctctt tttcggtact cgccggtggt ctgatatgca caatgatttc aaggacttcg
     4801 tagtcagagg ctcggatttt ttcccgcgat gggcccgcga gcttcgacaa aaacagagcc
     4861 gtgaacggat cgtcacacaa gcaaaaaatc gactggtaga ttcgtggcga cgcttctcca
     4921 ccgcggatta cgccgaggcg gggcgctcct ccctctcctc cgcccacttt ctactcaagc
     4981 acgcccagga ccgatgggag gaagcctccc aactatccca tcccgattat gtgctcatcg
     5041 aaaacgaagc acgaacagcc gctttctatt ctgatctttt cgccaacctc cgcgcttccc
     5101 acgcccaccg ctcgttttat ccctttgatt acgctatctt acacaaagtg cgtcgacacg
     5161 gcgccaccta cgagttgccg gaattgacca cgagcgtttg acccatcctc gagccaagag
     5221 aggattgacg tcacccggat ccgaccacag gttcagcctc gctttcgtgg taccgttatc
     5281 caactcatcg aactgaagga gtttttcatg tccaaggtca cctttcacgg caatcccgca
     5341 actctcgaag gaaagtttcc atccccagga gacaaagccc cgccctttac cctcgtcaac
     5401 ggcaagctcg aaaacatgga tttgacttcg ttttccggca agaaaaaagt ccttaccatc
     5461 aacccgagct atgacacccc ggtctgcgcc aaagcggccc gggaattcaa cacgcgagcc
     5521 gccaccatca acgacgccgt cgtgctcgcc atcagcgccg acttgccctt tgcccaaggc
     5581 cgattttgca cagccgaagg aatcgaaggc gtcgtgcccc tgtccgcttt ccgcagccca
     5641 acctttgcca aagactatgg cgtgctcatc accgatggac ccctggcggg actgacggcc
     5701 cgcgcggtgg tggtgatcga cgaaaacgat aaggtcatct actcggaact cgtcggggaa
     5761 atcgtcaacg agccgaatta tgaagcggcg ctggcatcgc ttcgataaac accttttcgg
     5821 ccttacctcg cgttaccctt cgggactacc ctttggacct gccagggtgc caccgcaagg
     5881 taattcgcat cccgcatagc gatttcgaaa cgtctccgct cgcttcgctt gccataattt
     5941 cccgttatcc cataccacat aggggggaat cgctttgatg aagcgccaag cggacgaaaa
     6001 agaataagcg ccttggccga cgacagcgag cacatctcct tcttcggggc tctgtcccca
     6061 tagggtatat ccccatacgt cgtgggggtc gcataagggc ccaaaaagcg tgaaacgtcg
     6121 tcgttcccgt gaggggcgtg tcacattgac gataggagcg aagcaatact cgaaaaatcg
     6181 atcgtcgccg agcatttggc ttcccccgtc gacgatggca ccacccggct tcacggcaag
     6241 gacggaaaga acgatatggg tcggatggga agcaatatat cgccctggtt cgaaaaagat
     6301 ctggatattc gggatgatag gttctagccg gcgccgtacg ctgtcgccga tccgttgagc
     6361 aaaaacatgg agaggttcga cggcggtgat tgtacatgcg taagggtcga acgcgggctt
     6421 cgctgaggtc atggacgagc ccaaacgaga agccagcgtt tgaagcagaa ttcctttgtc
     6481 ttcggcctta tgaagaaccg cttgcccttc cgggtaaaat ccccctccaa gatcgaggaa
     6541 ggacatgcgt tgtcgcacgg acgacggttg atcggcgagc caggcggcga tggtttcgat
     6601 gttgtttata tatttttccg gggttttatt ccaactcgag tgaacgtgga gaccgcacca
     6661 agtcagattg gggcaggttt gtgcttggtc gatggcgtag cgcagttgtt cgatcggaat
     6721 gccgaacttg gaccaggaag gatcctccga cggtgctgca cacaggcgaa aaccgatccg
     6781 caggggccgt gggggggcca tggctcgagc caggtcgatg atacgcttca gctcggagag
     6841 attatcgacg atgaggatga cgcggttggg gtgttgaagg gccagttcga gttcttcacg
     6901 cgtttttcct gggctgctga acaaaatggt gccgaatccg agttcgagag cgagttgcaa
     6961 ttcgaaaagg cccgcgacat cggcctgcaa gccttgctga tgaacgattt gcccgagagc
     7021 gggtaagtca ttgcttttca aggcataaaa tgcatggctg cggggaacgg acgtttgcat
     7081 ggtctgcacg aaatgggcgg ctatcgagcg aagctggtct gcctccaaaa aatattgtgg
     7141 agttccaaaa cgtcgggcgc tttccagcat tggctccatg ggaagcgaaa gaattcgtgg
     7201 gtcgaatgcg gtcggagccg catcgttgag cggttcgtcg agcaaggccc gtagttcgtc
     7261 tacccaagga ttcatggcct tgtctcgtag cgaacgattt ggggaaggat gttcatgagc
     7321 ttgtcgatgc cgctcgaatc ggacggctgg acgagcacgt aaccacggat gagaggcgtc
     7381 gaatcgacgt cggaagcggt tccttcggta tcgtagacgt gcgtagcgat gattcccagg
     7441 tcgtgggtgc gctcatgcaa accggaaagg tcgatgggag agttttcatg ccccgggggt
     7501 gcgatgagat acaggacggc accttcggag gaggggtaac ccagcgggat gggttcattc
     7561 atggcggcgg cgatcatgac agccaacgag gtgtatccca gtagttttgc catgaggtga
     7621 ttgaaggcgc tgaatccagg gcgaatggac gattcgagga cgtggatgct accccgatcc
     7681 actttgaaat ccaccatgca cacgccgtgg tggataccga aagcgcgaac gattcgatgg
     7741 catacggaga tcaaggtttc ctcatctatg ccgtgatgcg agaggcaagg gggtgggacg
     7801 agcaggtatc caccaaaaag tccgagcgtg ggaccgggca gttttcgagt gatgcgaagc
     7861 aaagcaatat gatcggcctg gacaaggaag tcacagctaa attcgattcc tccgacgaaa
     7921 gattcgagga gaaactgatg ttttgggtaa aggaactgtc cggaatcgga taatggctgc
     7981 ggttcgaagc gcggatccga aaaggtgttg agggttgctt ttttcaaacg agaaatattt
     8041 cggatcaggt ccttgggcga atcatttcga aacaggaatt ggctttgggt gccggacaat
     8101 actttgagaa cgttcggata gccgatggtc gccgcggcat cgagatcggc ttcggagagg
     8161 agagtgaagg ccgatgtggg gaccccgtgt tcgacgaatg cctgtttgca cagccatttg
     8221 ttggaagcca acgcgcacgt tcgagcgctg ggaaagggca aaccgaagtg ggtggcgagg
     8281 gtgcgggaca acgaaaaatg aagttcttca tcgatgccca cgaccgcttc gaattgagcc
     8341 tgatgagcgt gtgcccacgc atcggttttc tccacgaccc gctttgcatc agataggtcg
     8401 tcggcaatgc catgaagcgg cggaagaggc tcgagcaagg tggggggagc cacgacgagc
     8461 agacggtcga gttgatgtcg ttggtgcagg gattcgatgc agcatcgaag ttcgatggct
     8521 ccgattccgt aggcaggaaa aaggatgaag gattccatgg gaagagagcc acggtagcaa
     8581 ggggggcagg gggaagatag acagtgaaaa gaagctggat ttggcgtgtt ggcgggacgc
     8641 aagggaatca ttcggcgcgt tcgtgtccgc ctgccatctc gtcgagttcg gcttcgattt
     8701 cgtcgatggg tcggagggat tttccttgca tggggatgcc gcgggccacg gccacccgtt
     8761 cgagcgctcg ctgcaatccc tgacgatcca gaacggcggg cagccaaatg cgctcgcctt
     8821 cccaggagcg aagggcaacg ttcgaggatt cgaatacgat ttcatcgatt tccgcccagc
     8881 tcaagttctt gattttgggc caagacagac cgaaaacgtc tcggtattcg acaccgtcgg
     8941 gatggatgac cacgcctcgc atgctggttc ggagcatggc cgcgatgccc ccgagcagaa
     9001 ccaatacggc taacgtacgg gcaccgagga tgcgatgcga atcgccttcc acgatatagc
     9061 gaaacagcca actatccgac gtgtgcaggc ttgcggccat cacgagaccg acaaaagcga
     9121 tggcgagcac aaggtagagg gtgggaagga tccaggtgcg caacgggggg ccaaaggatt
     9181 cccgtccctg gggacgatag gtcatgtcga agccgggagg gcgggattct cgagccatgc
     9241 ctatggtgta ggacacgcag gggggggcag gcaataagcg acgaacgata gcggggcgaa
     9301 gggcggcaag agcaggaacc tccatggttt catagaatcg agatgggttt tcggaccgcg
     9361 gttcgcttgg ggaggcattc gggggcccga gagaattgaa aaggattttt ccaagcgaga
     9421 tggcgaggag agtagcgtga taatgtgtaa attcaatagg ttacttctta tgggggagtg
     9481 gtgtcacgaa tgtgtcgcgg gttttacggc tctttcggcg agccggatag gttcaccaat
     9541 accgggttgg cggtgtattc gataccggga tgatttccac attggccatg ttggttgttg
     9601 ccccggcaaa acacggcacc ctgactgtct acgccacaga ggtgagtcgt gctgtcgttt
     9661 ccggtgcctt cggcttgtac cacaccctgc aggggcgagc cggttccccg gtacgaccag
//
LOCUS       DAOSOR010000171         9689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_7012_length_9689_cov_5.337035, whole genome shotgun sequence.
ACCESSION   DAOSOR010000171 DAOSOR010000000
VERSION     DAOSOR010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9689
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7012_length_9689_cov_5.337035"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PK710_10945"
     CDS             complement(<1..539)
                     /locus_tag="PK710_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10281.1"
                     /translation="MKLRTTIWIAAGIASLSMGCKGSPASPFNQLQEQPIMVYRLQNN
                     QQPVATTTPGTATTPSLIPGLPGLPIPPEFQPQVQQAQSMICQMLPPGVPGCPTAGSP
                     TTQPVITPATPMFEGYAILGQSQVMDPKMREEIIDIFGYEKSFSSRKSPCMYPEFGMA
                     FGSGQTANLLISYSCNQVQP"
     gene            complement(686..1486)
                     /locus_tag="PK710_10950"
     CDS             complement(686..1486)
                     /locus_tag="PK710_10950"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOT10282.1"
                     /translation="MGPNPMLDLLRKALPLLLLTACDRGDGTARPRVAFGGASSVDSS
                     TPTPTEPFVEDKQTRREREELVRAIETRERISAKVAAALREVPRHAFVPQDQWHRAYE
                     DTPLSIGYGQTISQPTVVAMMTEALDLQGNEVVLEVGTGSGYQAAVLANLVRRVETIE
                     IIEPLAKNASNALRRLGIQNVHVHVGDGYAGLPSLAPFDRIILTAAPPEVPRALLDQL
                     RKGGKIVLPVGPRFGVQDLLVIEKGPDGRLEQRNLGAVRFVPMVRHDE"
     gene            1804..3057
                     /locus_tag="PK710_10955"
     CDS             1804..3057
                     /locus_tag="PK710_10955"
                     /inference="COORDINATES: protein motif:HMM:NF001614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOT10283.1"
                     /translation="MGQSFTQKILARGAGKSSVAISEIVDVTPDVVMSHDNTAAISAT
                     FKRMGVERLDDPRRHVIVLDHCVPAASEKYAQNHKTIREFVAEQGIEHFYDIQRGICH
                     QVLPEQGHVRPGALIVGSDSHTTTYGALGAFAAGIGRSETAAIMAIGRIWLRVPETMQ
                     VRVKGAFADRVGAKDFILRLIGDLGADGGLYQAVEFVGPAIDDMAVDDRLTLCNMTAE
                     MGAKNGYMLPDEKTRAWLQARGVKDYEPVFSDPDTVYARVLDYDVSALEPQLAAPHTV
                     DNVHPVKEWAGKPIHQALLGTCTNGRLCDLHRAADLLRGKKIDPRVRMLVFPASVEVV
                     REAMRDGTLLTLSEAGAVIMNAGCGPCLGAHEGILAPGEVCISTANRNFRGRMGSRDA
                     DVFLASPETVAASALTGQITDPRTL"
     gene            3076..3576
                     /locus_tag="PK710_10960"
     CDS             3076..3576
                     /locus_tag="PK710_10960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOT10284.1"
                     /translation="MTIQGKVWKYGNDVNTDVIFPGKYTYTITDRKEMAAHALEDLDP
                     TFAKEVQAGDIVVAGSNFGCGSSREQAATCLKEAGVAAVIAASFARIFFRNAINEGLP
                     LIECPEAIGAIEAGQTVEVDFEGGILRVGDKTYQFPGLPESVMGIVREGGLIPYVRKQ
                     LAQRKA"
     gene            3707..4888
                     /locus_tag="PK710_10965"
     CDS             3707..4888
                     /locus_tag="PK710_10965"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOT10285.1"
                     /translation="MASLTVVTMPGDGIGKTVLPESLRLLDAAGFKADYVHADIGWDF
                     WCKEGNALPERTVALLEKHRLGLFGAITSKPKGDALKELDPALRDKGYVYFSPIVAMR
                     QRFNLDVCIRPCIAFPGNPLNFIRREGDGFVEPRVNAVIFRQNTEGMYAGVEWTDPPE
                     AVRGALQSHPKMKFFSSVEGKDLAISCRIVTRGACQRIARAAFEYAKTFGYRSVTVCE
                     KPNVLRETSGMMESVAKEVSADYPGIALWSTNIDAQMMWLTKNPEDYGVVVATNLFGD
                     VISDAFAGLVGGLGFACSANTGKDCAVFEPTHGSAPKYQELNPSIVNPIAMHLSACMM
                     LDHIGERGMADRIRGAIGAVIQEGKVRTYDMMRLTGGPKVFEKGACTTQQMTDAIIKK
                     L"
     gene            4898..5473
                     /locus_tag="PK710_10970"
     CDS             4898..5473
                     /locus_tag="PK710_10970"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT10286.1"
                     /translation="MIRAEDIQQLFGSQLARIENTSLRENVVKAWVIGCQRGGYENVD
                     QLLAMPFTLLTDCRGVTFVEHTIAVTEGSIGLAKAQHEAYRSMPYVIDMDRLIAGALL
                     HDVGKLVEIQQDGSGGFVKSRNGKCMRHPISGTVIAAEAGLPDEVLNIIACHAKEGEG
                     RPQVVETVLIHLADFATFDPLVMKAKGSLIE"
     gene            complement(5656..7266)
                     /locus_tag="PK710_10975"
     CDS             complement(5656..7266)
                     /locus_tag="PK710_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10287.1"
                     /translation="MRIVVLPLLCLPLCSCSTDDDSNQAPWPDAADASIAESGLEDQT
                     QESLPDITTDIPTDTAQDRIEEPVNSDSIDDVLTDNLEPIDVDPDTSCPGLVAEPDVD
                     PFLMRSASAGFGGVETVSSGGHQDVFLRSPGQLTRIGVRLDWGGTVVFFGLSANPASN
                     VIDANDTGRELQIALYDPTRIRQPCAWNASCLTSSESCGNSITFLGWNPVQGGDECNR
                     GAPVLSHGAVGDALQVVIQPLQWNPDWDRKDCTQTPCGGPGRPVDVTYQMHYRYLSEH
                     VVEIATQVSSQETISHPVTGQEWPTLYVSHGKNGPDLPVLLKADGQVVTIANPANDGF
                     FFENFDSPAPWVSFQNTLRDYGVGLAMDQGTRQYQGWHGNGVTAPYFHNVRARLAFGL
                     DAGAVVRGVSYLALGSFDTVKYELESVLSKRAPFGACEQPKSGSVHSFAKGSPIVVQG
                     WVLDNAPGTTIRVTIDDVLVASFAAEQERPDVCRTYPAYASCPNVGFQHSISTTSLDA
                     CAHLMRIIAEDIDGNARVLAERIIREQG"
     gene            7285..8280
                     /locus_tag="PK710_10980"
     CDS             7285..8280
                     /locus_tag="PK710_10980"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOT10288.1"
                     /translation="MRVGRTRDDKGKDFGGRPSSRYSAWVRWIHLGIGLGTLAIVSGL
                     AWLAVATRGCGRTKNGVDSEKEASQWPAPEASLHAPIIRAARGMTKIRLFVDPGHGAE
                     GNTGNLSCFCEKEQDFTLDLAKDMGTYLQQTGRFEVMLSRGVGEVVSYPDRVSASERW
                     KAHVFLSLHSDVRGRHESWTLNDGRTCPRSESAPGFSVLFSDEGGLATQRHILAQSLA
                     KRMSQAGFLAYLGEQYSMYEADPQVQGVFADRHPLDKRIFVLRQPSMPSILVETHNAW
                     DPREVRRWDERHTRTVFFQVVEATLIDFMTARPGELSSEKDKKASLPEADDAGGR"
     gene            complement(8287..9366)
                     /locus_tag="PK710_10985"
     CDS             complement(8287..9366)
                     /locus_tag="PK710_10985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT10289.1"
                     /translation="MLFVLPPFVKRVGGLHVAQCIEEAQQTVRSHLIHILLIGGSLAI
                     DGGDVLVRSLRERDPHAQVMVLRERNGEHLPTSFLAEGICDILHKPFDVARIPKQIHR
                     LVEVRNELERRAKMQRDADIRLRHADRMILLGTLVATVAHEVANPLSAIATNTSLVHE
                     LLSNSASAEDIPMLVEASKDSLSSIALIRDYLSRILRFARRDGASSFESDLANTLKTA
                     LLIVRCRAQDKNILLHADAMLPPPKVPHHPTLLAQAVVNALTNAIDAVQEHGNIWLRI
                     EECDDEVVVIVEDDGPGFAFDAKHRILDPFFTTKESGTGLGTTVIRQVMLEHQGTAEW
                     KARDSGGIQVRLRLPRHPVSHRTYK"
     gene            9522..9653
                     /locus_tag="PK710_10990"
     CDS             9522..9653
                     /locus_tag="PK710_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10290.1"
                     /translation="MIRVPIRPVSARPLLHERHLLICATLATDRVVCTAIPAKEVRT"
BASE COUNT         2159 a         2725 c         2781 g         2024 t
ORIGIN      
        1 ggctgaacct ggttgcacga ataggagatg agcaagttgg cggtttggcc gcttccaaaa
       61 gccattccga actccgggta catgcacggg cttttccggc tggagaagct cttttcatac
      121 ccgaaaatat cgatgatctc ttcgcgcatc ttcgggtcca tgacctggga ttgaccaagg
      181 atggcatagc cttcgaacat cggagttgca ggcgtaatga ccggttgtgt ggtgggagat
      241 ccggccgtgg ggcagccagg aaccccagga ggcagcatct ggcagatcat actctgtgct
      301 tgctgcacct gcggttgaaa ctccggcgga atcggaagac caggtagacc cggaatcaga
      361 ctcggagtgg tagccgttcc tggagtggtg gtggcaaccg gctgctgatt gttctgcaga
      421 cgataaacca tgatgggttg ttcttgaagc tggttgaagg gggaagcagg agaacccttg
      481 catcccatgg acaaggaagc gattccagca gcgatccaga tagtggtacg aagtttcatg
      541 ggcaccgatt ctagcagtgt ttggagaaag gagaagaatt cctttccacg agttgaatgg
      601 tttttagacg gtcggacaag cctgattgtt caacggctgt aggatggtat ttcaaactcc
      661 cttccgcaat cccttgcccc ctcaatcatt catcgtgtct taccatggga acgaaacgga
      721 cagcgccaag gtttcgttgt tcgagacgac cgtcaggtcc tttttcgatg acgagcaagt
      781 cctgcactcc aaatcggggg cccactggca agacgatctt tcctcccttt cgtaattgat
      841 cgagaagggc tcgaggtacc tcaggcggcg cggcagtcag gatgatgcga tcgaaaggtg
      901 ccaacgaagg caatcccgcg tagccgtcgc ctacatgaac atgcacattt tggatgccaa
      961 gacggcgaag ggcgttgctt gcgttcttcg ccaacggttc gatgatttcg atggtttcca
     1021 cccgtcgtac gaggttggcc aataccgcgg cctggtagcc ggagccagtc cccacctcca
     1081 acacaacctc gttgccctgc aagtccaacg cttccgtcat catcgccaca accgtcggtt
     1141 gggaaatcgt ctgcccatac ccaatcgata aaggcgtgtc ctcgtacgca cgatgccatt
     1201 gatcttgggg aacgaaagcg tgacggggca cctctcgcaa agccgctgct acctttgcgc
     1261 ttatgcgctc gcgcgtttcg atcgcgcgca ccaattcctc tcgttcacga cgggtttgct
     1321 tgtcttccac aaaaggttct gtcggtgtag gagtcgagga atccacggaa gaagcgcctc
     1381 cgaaagctac acgaggccgt gccgtcccat caccgcggtc gcaggcggtc agcagcaata
     1441 acggcaatgc ctttcgcagc aagtcaagca tcggattcgg tcccatgagc ttgtcgccca
     1501 caagacaccg ggtcaaccat gcaaaagggg ggcgaaaacc gaaacctcac cgaaccaatc
     1561 ctaaaaaaca caataaattt caacttgtta ccgatattgc ctcgatcctt cgacggcaaa
     1621 cccatgcttt gcggcggtcc gggtcttccc gttcgatgac gacacaatcc gggttgacct
     1681 ttgccacatc ctccgctttg ctgcgcacca ttgcaggatg ccgcacccaa ggagataccc
     1741 gactccttcg gcatcgcctt cagaccatcg ttttcgtccg ctcgtcaacc cagagaggtc
     1801 gccatggggc aaagctttac ccagaagata ctggctcgcg gagcaggcaa gtcctccgtg
     1861 gccatttccg aaatcgtcga tgtcacgccc gatgtcgtga tgtcgcatga caacaccgcc
     1921 gccatttccg ccacgttcaa gcgaatgggt gtcgaacggc tcgatgaccc acggcgccat
     1981 gtcatcgtcc ttgatcattg tgtgcccgcg gccagcgaaa aatacgccca gaaccacaaa
     2041 accatccgcg aattcgtggc ggaacaaggc atcgaacact tctacgacat ccagcgaggc
     2101 atctgccacc aagtcctccc cgaacagggg cacgttcgtc caggagcctt gattgtcgga
     2161 tccgattccc acaccaccac ctacggcgct cttggcgctt ttgcagcggg aattggacgc
     2221 agtgagacgg ctgccatcat ggcgattggt cgcatctggt tacgggttcc cgaaaccatg
     2281 caagttcgag tcaagggcgc gttcgccgat cgcgtgggcg ccaaggactt cattcttcgg
     2341 ttgatcggag acctcggtgc agatggcggt ctttaccaag ccgtcgagtt cgtgggaccg
     2401 gccatcgatg atatggccgt ggatgatcgt ctcaccctct gtaacatgac cgcggagatg
     2461 ggcgcgaaaa atggatatat gcttcccgat gaaaagaccc gggcctggct acaagcacgc
     2521 ggagtgaaag actacgaacc tgtgttcagc gatcccgata cggtgtacgc gcgcgtgctc
     2581 gactacgacg tatccgcgct cgaaccgcaa ttggcagcgc ctcataccgt ggacaatgtg
     2641 catcccgtga aagaatgggc aggcaagcca attcatcagg cgctgcttgg tacgtgtacg
     2701 aatggacggc tctgtgattt gcatcgcgca gcagatcttt tgcgaggaaa aaagatcgat
     2761 ccacgcgttc gcatgcttgt gttcccagcc tcagtcgaag tcgttcgcga ggccatgcgc
     2821 gatggcacgc tgctcactct ttccgaagct ggagccgtga tcatgaacgc cggatgtggc
     2881 ccttgtctcg gcgctcacga aggcatcctt gcaccaggag aagtctgcat ttccacggcc
     2941 aatcgcaatt tccgaggacg catgggcagt cgagacgccg atgtctttct ggcaagcccc
     3001 gagaccgtcg cagcttccgc cctcaccggg caaatcaccg atccacgaac cctctgatcg
     3061 cgcaggatta cgctcatgac cattcaaggt aaggtttgga agtacggcaa tgacgtgaat
     3121 accgacgtca ttttccctgg aaaatacacc tacaccatca cggaccgcaa agagatggcg
     3181 gcccacgcct tggaggactt ggaccccacg ttcgccaagg aagtccaagc aggggatatc
     3241 gtggtggcgg gaagcaactt cggctgcgga tcgtcgcgtg agcaggcagc cacatgcttg
     3301 aaagaagcag gggtcgctgc cgtgatcgcg gcttcctttg ccaggatttt ctttcgaaac
     3361 gccatcaacg aagggttgcc attgattgag tgccccgagg ccatcggtgc gattgaagca
     3421 ggtcagaccg tggaagtgga cttcgaaggg ggcatcctgc gggtcggaga caaaacctat
     3481 caatttcccg gtttgcccga gtcggtgatg ggcatcgttc gcgaaggcgg cctcatccct
     3541 tacgtccgca aacagctcgc ccagcgaaag gcgtaagcaa tcggtacgcc ccccgctgat
     3601 tccctttgac gcaaccgcac ttcagatcca cgcttccgcc ctcttcggga caccggcgat
     3661 gagagatgcg cggcgtcccc ctcacgcaaa tcattcagga ggatccatgg cgagtctcac
     3721 cgtcgtgacg atgcccggtg acggcattgg caaaaccgtg cttcccgagt cccttcgatt
     3781 gctcgatgcc gccggcttca aagccgatta cgttcacgca gacattggtt gggatttctg
     3841 gtgcaaagaa ggcaacgccc ttcccgaacg taccgtggcg ctgctcgaaa aacaccgtct
     3901 cggactgttt ggcgccatca cttccaaacc caaaggagac gctctcaaag aactcgatcc
     3961 cgctttgcgc gacaaaggct acgtgtactt cagtcccatc gtggccatgc ggcagcgctt
     4021 caacctcgac gtctgcattc gcccctgcat cgcattcccc ggcaatccgc tgaacttcat
     4081 tcgtcgtgaa ggagacggat tcgtcgaacc gcgagtcaac gccgtcattt ttcgccaaaa
     4141 tacggaagga atgtacgccg gcgtcgagtg gaccgatccc ccggaagccg tgcgtggcgc
     4201 gcttcaatcg catccgaaga tgaagttttt ctcctcggtg gaaggcaaag acctcgcgat
     4261 ctcctgccga atcgtgactc gcggcgcttg ccaacgcatc gctcgcgcag ccttcgaata
     4321 cgccaaaaca ttcgggtatc gttcggtcac cgtctgcgaa aaacccaatg tgctgcgaga
     4381 aacctcgggc atgatggaat cggtagcgaa ggaagtttcc gccgattacc caggcatcgc
     4441 cctttggtcg accaacatcg atgcgcaaat gatgtggctg accaaaaacc ccgaagacta
     4501 tggcgtcgtc gtcgccacca acctgtttgg cgacgtgatt tcggatgcat tcgccggatt
     4561 ggtgggaggt cttggcttcg cgtgcagcgc caataccgga aaagattgcg ccgtcttcga
     4621 acctacccat ggctccgccc ctaaatacca agagctgaat ccgtccatcg tcaatcccat
     4681 cgccatgcat ctttcggctt gcatgatgct cgatcacatc ggagagcgcg gcatggcgga
     4741 tcgaatccgc ggtgctatcg gcgccgtcat tcaagaaggc aaagtgcgca cctacgatat
     4801 gatgcgactt actggaggtc cgaaagtatt cgaaaaaggc gcatgcacta cccagcaaat
     4861 gaccgatgcc atcatcaaaa aactctgagg tgacatcatg attcgagcag aagacattca
     4921 acaactgttc ggaagccaac tcgcccgtat cgaaaacacg agcctgcgcg aaaatgtggt
     4981 caaagcatgg gtcatcggct gccaacgagg cggctatgaa aacgtcgacc aactgctcgc
     5041 catgcccttc accctgctca ccgattgccg aggcgttacg ttcgtcgagc ataccatcgc
     5101 cgtgacggaa ggctccatcg gcctggcgaa agcgcagcat gaagcctacc gatccatgcc
     5161 ttatgtcatc gatatggatc gactcatcgc cggagccctg ctgcacgatg tcggcaaact
     5221 cgtcgagatc caacaagatg gttccggcgg attcgtcaaa agccgaaatg gcaagtgcat
     5281 gcgtcatccc atctcgggga cggtgattgc cgcggaagcg gggctgcccg acgaagtgct
     5341 caacatcatt gcctgtcacg ccaaagaggg agaaggacgc ccccaggtgg ttgaaacggt
     5401 cctcatccat cttgcggact tcgcaacctt cgacccgttg gtgatgaaag ccaagggaag
     5461 cctcatcgaa tagtcggcgg ccttgtgtct tcgccccttc caaaaccttt tcttgcagcc
     5521 tcctaccacg gtctcgtcgc gagccattcc cttctcggag gggaagctgg cgaaagaaaa
     5581 ccttgtcggt aacaattatt atctaataaa atcaatagct tgcaacgatt gtatccttgc
     5641 tggtctccgg ctgcctcatc cttgctcgcg gatgatccgc tctgccaaaa cacgcgcgtt
     5701 gccatcgata tcctcggcaa tgattcgcat caaatgcgcg caggcatcca ggctcgtcgt
     5761 ggagatcgaa tgctgaaaac caacgtttgg acacgatgca taagccgggt aggtgcggca
     5821 aacgtcggga cgttcttgtt cggcagcgaa ggaggccacg agaacgtcgt cgatggtgac
     5881 gcggatcgtt gtgcctggag cgttgtcgag gacccatcct tgcacaacga tgggcgagcc
     5941 tttggcaaaa gaatggacac tgccactttt cggctgttca caggctccga acggcgcgcg
     6001 tttggataag acggattcga gttcgtactt cacggtatcg aaggaaccga gggcgagata
     6061 cgacaccccg cgaacgacag cacccgcatc gagaccgaag gccaatcggg cgcggacgtt
     6121 gtggaagtac ggtgcggtga cgccattgcc gtgccagccc tggtactggc gcgtgccctg
     6181 gtccatggcc aagccgactc cataatcgcg caacgtattt tggaaggata cccagggggc
     6241 gggagagtcg aagttttcga aaaagaaacc atcgttggca gggttggcga tggtgacgac
     6301 ttgcccgtcg gccttgagca acactggcag gtccgggcca tttttcccat gggacacgta
     6361 tagcgtaggc cattcttgac ccgttaccgg atggctgatg gtttcctggc tggatacctg
     6421 cgtggcgatt tcgaccacgt gctcggacag gtaacgatag tgcatttgat aggttacatc
     6481 gacaggtcgt cccggtcctc cgcaaggggt ttgggtgcaa tcctttctat cccaatcggg
     6541 attccactgc agaggctgaa tcacaacctg tagcgcgtca ccgactgcac catgggatag
     6601 aacgggcgcc ccacgattgc attcatcccc tccttgtacg ggattccatc cgagaaaggt
     6661 aatggagttg ccgcaggact ccgacgatgt caggcacgat gcgttccagg cgcaaggctg
     6721 gcgaatccgc gtggggtcgt acaaggcgat ttgtaattca cggcccgtgt cgttggcatc
     6781 gatcacattc gaggcagggt tggcgctcaa tccgaaaaac accacggttc ctccccaatc
     6841 gaggcggact ccgatgcgcg tgagctgtcc ggggctacgc aggaaaacgt cctggtgccc
     6901 tccgctcgac acggtttcga cgccaccaaa ccccgcgctc gcggaacgca tcaaaaatgg
     6961 atcgacgtcg ggttcggcaa caaggcctgg gcaggaagtg tcagggtcca cgtcgatggg
     7021 ctcgagattg tccgtgagga catcatcgat agagtcggag ttgacgggtt cctcgatccg
     7081 atcttgcgcg gtatccgtgg ggatatccgt ggtgatatcc ggcaaggact cttgggtttg
     7141 gtcttcgagg ccggattccg cgatcgaggc atccgcggca tcgggccagg gcgcttggtt
     7201 cgaatcatca tccgtggagc aagagcataa ggggaggcaa agaagcggaa gaacaacgat
     7261 tctcatctgg caaagataag acgaatgcgt gtggggcgca ccagggatga taagggcaag
     7321 gattttgggg gccggccctc gtcgcgttac agtgcttggg tgcgttggat tcatcttgga
     7381 attggtttgg gaacgctggc gattgtcagt ggacttgcgt ggttggcggt ggcgacacga
     7441 gggtgtggaa gaacgaaaaa cggggttgat tcggaaaagg aagcatcgca gtggccggcc
     7501 ccggaggctt cgcttcacgc ccctatcatt cgcgcggcgc gaggaatgac aaagattcgg
     7561 ctgtttgtgg atccaggaca tggtgccgag ggcaacaccg gcaacctatc gtgcttttgc
     7621 gaaaaagagc aggacttcac gttggatctt gcgaaggata tgggcacgta cttgcagcaa
     7681 acagggcgct tcgaggtgat gctaagtcgt ggcgttggtg aagtggtgtc ctatccggat
     7741 cgggttagcg cctccgaacg ttggaaagcg catgtttttc ttagtctgca ctcggatgtg
     7801 cgtgggcgac acgagtcatg gacattgaat gacggaagga catgcccgcg aagtgagagc
     7861 gcaccagggt tttccgtgct gttttcggat gaaggcggtc tggcaaccca gcgccatatc
     7921 ctggcgcaaa gcctggcgaa gcgaatgtcc caagccgggt ttcttgccta cctaggggaa
     7981 caatactcga tgtacgaagc cgatccgcaa gtgcaagggg tcttcgcaga ccgccatcct
     8041 ttggacaaaa ggatattcgt acttcgtcag ccaagcatgc catcaatcct cgtggaaacc
     8101 cataacgcct gggacccacg agaagttcgc aggtgggacg aaagacatac tcgaacggtg
     8161 ttttttcaag tcgtggaggc tacgctcatc gatttcatga cggcccgccc aggggaattg
     8221 tcgtcggaga aagacaagaa agcgagtttg cccgaggcgg acgatgccgg gggtaggtag
     8281 aaaaaatcac ttataagttc ggtggcttac gggatgacgg ggaagtcgta agcgcacctg
     8341 aatccccccg ctgtcgcggg ctttccattc cgcggttcct tgatgctcca acatgacctg
     8401 gcgaatgacg gtggtgccca agcccgtgcc ggattctttg gtcgtgaaaa acggatcgag
     8461 aatacgatgc ttcgcgtcga aggcaaaccc agggccatca tcttccacga tgaccaccac
     8521 ctcatcgtcg cactcctcga ttcgcaacca gatattgccg tgttcttgta ccgcatcgat
     8581 ggcgttggtc agagcgttga ccaccgcttg cgccaatagc gttgggtgat gaggcacctt
     8641 cggggggggc agcatcgcgt ccgcatgcag aaggatgttc ttgtcttgcg cgcgacaacg
     8701 cacgatgagc aacgcggttt tcaacgtgtt tgccaggtcc gattcgaagc tggaagcgcc
     8761 atcgcgacgg gcaaatcgaa gaattcggga gaggtaatcg cggatgagcg caatcgacga
     8821 caaggaatcc ttggaagctt ccacgagcat ggggatgtct tcggccgaag cggaattgct
     8881 gagcaactca tggacgagac tggtgttggt agcgatggcg gataggggat tagccacttc
     8941 atgggcgacc gtggccacca gcgtgccgag taaaatcatc cggtcggcat gtcgaagacg
     9001 aatatccgca tctcgttgca ttttggcgcg acgctcgagt tcgtttcgca cctcgaccaa
     9061 tctatggatc tgtttgggga ttcgggccac atcgaagggt ttgtgcaaga tgtcacatat
     9121 gccttcggcg aggaaagacg tggggaggtg ttctccattt cgttcgcgta agaccatcac
     9181 ttgcgcgtgg ggatctcgtt ctcgaaggct acgcacgaga acatcgcctc catcgatggc
     9241 aaggctgcct cctatcaaca ggatgtggat cagatgcgaa cgaaccgtct gctgagcttc
     9301 ctcgatgcat tgcgcaacat gcagcccgcc cacccgtttg acgaagggtg ggaggacaaa
     9361 aagcacctca gaatcattgt cgcaaagcag aatcgaaggg cgtgattgat cacccggtca
     9421 aactgacgct caagcaaacc ggtctttgag ccgttgctga ggggtttacg tgatagcgcc
     9481 atctcgtcgg gtcgccgcca ctgccacatc atggcggcga gatgattcgc gtgcccatcc
     9541 gccccgtgtc ggctcgcccc ctcttgcacg aacggcatct gctcatctgc gctacgctgg
     9601 cgacggatag agtggtatgt acggccattc ccgccaagga agtccgcaca tgaccaccac
     9661 cgattctctg ttctcatacg cccccagcg
//
LOCUS       DAOSOR010000172         9678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_7021_length_9678_cov_4.463889, whole genome shotgun sequence.
ACCESSION   DAOSOR010000172 DAOSOR010000000
VERSION     DAOSOR010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9678
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7021_length_9678_cov_4.463889"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="PK710_10995"
     CDS             <1..1170
                     /locus_tag="PK710_10995"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT10291.1"
                     /translation="GGESVGSSWLRCLWTTTASVLIDHGCAYLGWSGSFADGRWSRIR
                     GNKGFGDTLSVDGASNRCLGIVAGRASHAASAAPMMDGERAFWHDCNPFTHQWGPGIQ
                     CVIAGMIRLEPGSIVASAFSIVRLIGSGSMGTVYEARVIETGGRVALKFPHPAFGSSK
                     SALQRFHREAHATLAIDSPHVVRTYAVVKLKRGMPVLVMEYVDGTELADLIDRHEGPI
                     PLPLVLLLVKQIAIGLSAAHGAGVIHRDLKPSNVLVRNLRTAPVAKVFDFGLSVILSE
                     GGSRLTFSGSSFGTPQYMAPEQIHDTKHVDERADIYALGVMTYELLTGRWPFSGTTTQ
                     EIWHDALNSQPIALRSRRPELSQELCDIVMRAMARSKEDRFATADEFREALLPFF"
     gene            1194..1991
                     /locus_tag="PK710_11000"
     CDS             1194..1991
                     /locus_tag="PK710_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10292.1"
                     /translation="MSTTNGNPQKSTNTASSEDEDKQARIALQRIITMSLLAGLCPAI
                     PIPFLDDMALRFIRKRALRSEFERQGLRPGRAQLDVFLQERSRLLGCLSSILIYPIKK
                     LFRKVSFVLAVKDCVDAASWTFHTLWMVRHAMVKGLFSNADLGVEVDALRGLRLAVDA
                     ASQQVDTRPFHQMWKRALGASRAWIRQGGRALGKMIRFKGGRSTAPETVERAVQDVEP
                     LDIGEVDEVANRLGEQMWAERGYLRAVERAFETHWARYRAASASPTS"
     gene            2025..2825
                     /locus_tag="PK710_11005"
     CDS             2025..2825
                     /locus_tag="PK710_11005"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOT10293.1"
                     /translation="MLICNERQISDDFSFPGIHLFMAHVAFSRLIGVIVVAGLTSCRT
                     SETISESSERAPSPRISDSPDASDSAQAPPRHAPPVVTGKAELGKPAPLFSLLDEAGK
                     TVSLTDYRGKIVVLEWFNPFCPFVKASHERGSMKPAIEKAERQGAVWLAVNSAGPGKQ
                     GHEAVANAAAKKQWNMNYPVLMDATGEVGRAYGAQRTPHMFVIDSAGILVYMGAIDNS
                     PDGDRGSPKDGVLVSHVEEALEDIAMKRAVRTASTEAYGCTVKYGGDR"
     gene            2974..3465
                     /locus_tag="PK710_11010"
     CDS             2974..3465
                     /locus_tag="PK710_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10294.1"
                     /translation="MARAQPEPPPQHIPTDVHPPSPVRWKLVMGGLATTAGFYAVAQP
                     FSYAWPDSPGSRDLRIPVAGPWISVTNIRCRDTDPDCSGFLLWMRGIFTVLDGLGQAG
                     GLLISLEGALLETAEPSVDPSPKPRQEPSPKDEPPPRNLFIGPTPMGVGDRGLGISVS
                     GAF"
     gene            complement(3490..4347)
                     /gene="amrB"
                     /locus_tag="PK710_11015"
     CDS             complement(3490..4347)
                     /gene="amrB"
                     /locus_tag="PK710_11015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOT10295.1"
                     /translation="MTHSPTTTFGPTVAGRFYPQNPRELRSSVESFLAEGKSSPALGP
                     ARRIVALVAPHAGYVYSGSIAGMAYAAAQPQSVRTVVVLSPSHHGRRPFVCTLSADAY
                     DTPLGTVPIAKSIVDALVAKGDLVQYDDALFRPEHALDVHVPFVKVAFPGAQLVPLIV
                     PMMPRDRLEGLGKLLFQVIGHDPHALLIASSDLSHFYSYDEARTIDEAIVSELERNDI
                     ETVFLKHDERRGPCGIAPIMTAWAYARNFGSTGLVHRLSVKNSGDVYREGRDRVVGYA
                     ALALSVADP"
     gene            complement(4513..6153)
                     /locus_tag="PK710_11020"
     CDS             complement(4513..6153)
                     /locus_tag="PK710_11020"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOT10296.1"
                     /translation="MKEPESRPMQPCGDSNAHSGCGEDNSAEGRFHALVRLTRRLTDT
                     HELQELLPLVVREVLSVLKMERGVLLLDEGAGGYEAVVSCDNKDSGAYSPTDQDATRA
                     LFEEVKATRRPVIPCSRKASEGDSSFWNLGTVVGLPLLDRSRFLGVLCLDSATKHIQI
                     GGDGLPAYLAFLESVAAVAALAVTNARAHTRMLEAQKLSEDFGNLFDLDEVFDRALSR
                     IVQLTRAEQGFLFIRGPTGQLEQRVGLDRKGRYLPESEDRFVSKRIIRAAAETGEVIL
                     SDNVAEVDAFGGSDGSVIRLGLTSILCVPIRGRDSTLGVVYLENRLLIGVFDEEEKRL
                     VQLVADRAGTAIEAAKLLRQQRDILRALANAVEARDVGTSSHVQRVSHFAIAVGHCLG
                     LPNEQLMLLEQSALLHDIGKIGIPDRVLLKQGPLCEEEWRLMKEHPQLGVGIVSPVDL
                     PKEVLDGILYHQEAWNGRGYPFGLSGENIPLFGRIIAVVDAFDAITTDRPYRKARTIR
                     QAADELSRCAGEQFDPEVVWAFLSVLNEAMETDTALRP"
     gene            6384..7850
                     /locus_tag="PK710_11025"
     CDS             6384..7850
                     /locus_tag="PK710_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10297.1"
                     /translation="MGFFETLTGKKTFCSVCGVPVGSSCLRDATGEPLCSEACRDAMQ
                     RFSNAPPAMENAPEAAELTGKAPASPSPFPHPELVVFVFRTNTATAFFSLQDELIVAS
                     GEPKLQVQMWEGQGVSLVVVNGPAGSIQTYRRTLRTNLGRLAKAAKCRPERIRPDVTY
                     SNVSEMGEYHPDDKAIQRVCDKIRRGAFRRRSSTVLTSPAESTSPPTPRPRTASDQSL
                     LRNAIFQIDKHVTFDAKGEPQVQLEGVREAARLLEQLADRHPSDPEPATLETSAVLLD
                     TQHARCSTCIETLVQRFPDHAEARMIAMTRKSIFAFPPYDVGASLPDFLRKRIRGATL
                     QVVRQGCLAHAVLFVESSTAYPTSPAPVAYPLYVQSAGIPVFGVAVVFADGSRVETTV
                     AGFEEHDGHWSLAPKACYLLCADTLPVVTLVPWDAGSPKRANEPLSVAYAVFDPTSRE
                     AFVGCEATYRGLQPRAFPLHELSSALQHFRDKISSIGK"
     gene            7984..8760
                     /locus_tag="PK710_11030"
     CDS             7984..8760
                     /locus_tag="PK710_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10298.1"
                     /translation="MKAYWLGWIGLGALLALTGAKGDGCQPAPQQGEVCGVGDACAVG
                     LTCVHYYGIAGPSGPEFRSCEIPCTQGSDCPKDQACTLIADGPGHVCRPSSDAPCDPS
                     VEQSCGANEVCVADTLTDQGFCHLAGHGQSVGAGYACGGSIGNTCAQGLYCKGLAHDV
                     HGGTGVCAQMTCDDWRTEFDNFLASMQGCVTAEDCAAVSGTSCGCTRNVVVNKNGDYE
                     GFVAFVQQMNDAGCGRRTPCDCPAAQGFACVDGICGWNYQ"
     gene            complement(9084..>9678)
                     /locus_tag="PK710_11035"
     CDS             complement(9084..>9678)
                     /locus_tag="PK710_11035"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOT10299.1"
                     /translation="SKPLPIEVLSDRVMDHTLRKHVSMALGLVISFTAASAFAQDSDT
                     PGIGLGGDAAVFMPIGNLGDGVGPMLGALLKLEVPVNPGLEVTGRAGYLYGLEKNDCC
                     SMSDIPIWAGVRYFFDGSFENAYVGGELGLNMVTAKVEVMGVSASASDTDFGAGLSVG
                     FKPGDLDVRGTFSMLDLGHAGDSMLVGASVGYTFTHF"
BASE COUNT         2047 a         2574 c         2958 g         2099 t
ORIGIN      
        1 ggtggggaaa gcgtgggttc gtcatggttg cgttgtcttt ggacaacgac agctagcgtg
       61 ctcatcgatc acgggtgtgc gtaccttgga tggagtggat cgtttgcaga cggacgatgg
      121 agtcgaatcc gtggaaacaa ggggttcggc gatacccttt ccgtcgacgg cgcgtcgaat
      181 cgttgccttg gcatcgttgc cggccgagcc tctcatgctg cctctgcggc gccgatgatg
      241 gatggcgaac gggcattttg gcacgattgc aatccgttca cccatcaatg gggacctggt
      301 atacaatgcg tgattgcagg aatgattcga ctcgaaccgg gaagcattgt agcgagtgct
      361 ttttcgattg tgcggctcat cggctcgggg tcgatgggta cggtgtatga agcgcgagtc
      421 atcgaaacgg ggggacgcgt tgcgctaaaa ttccctcatc ccgcatttgg ctcgagcaag
      481 tcggccctcc aacgtttcca tcgagaagcg catgccacgc tagcgatcga ctctccccat
      541 gtagttcgca cgtacgcggt ggtgaagctc aagcgtggaa tgccggtatt ggtcatggag
      601 tacgtggacg gaacggagtt ggccgacctc attgatcggc atgaaggtcc gattccccta
      661 cctttggtgt tgttgttggt gaaacagatt gcgatagggt tatcggcggc gcatggcgct
      721 ggggtgattc atcgagatct caaaccaagc aacgtgcttg tgcgcaacct gcgaaccgca
      781 ccggtggcca aggtctttga ttttggtctt tcggtgatct tatcggaggg gggcagtcga
      841 ttaacgttca gcggttcttc ttttggaaca ccccaataca tggctccaga gcaaatccac
      901 gataccaagc atgtggatga gcgcgccgac atctatgctc ttggcgtgat gacgtacgag
      961 ttactcacgg ggcggtggcc gttttcgggg actacgacac aagaaatttg gcatgacgcg
     1021 ctcaacagcc agcccattgc gttgcgatcg cgtcgtccgg agctttccca ggagctttgc
     1081 gacatcgtga tgagagcgat ggcacgatcg aaagaagatc gtttcgcgac ggctgacgag
     1141 tttcgtgaag cgttgcttcc gtttttctga acctcggtcc ggggatcagg gtgatgagta
     1201 cgacgaacgg gaatccccaa aaaagcacca atacggcttc ctccgaagac gaagacaagc
     1261 aagctcgaat tgcgctgcag cgcatcatta cgatgagttt acttgcaggg ttatgcccag
     1321 cgattccgat tccattcctg gatgacatgg cgcttcgatt catccgaaag cgcgccttac
     1381 gttcggagtt cgagcgtcag gggttacggc ctgggcgtgc tcaactcgat gtatttttgc
     1441 aagaacgatc gaggctgctt ggttgccttt cgtcgatctt gatctacccg atcaaaaaac
     1501 tctttcggaa ggtgtcgttc gttcttgcgg tcaaggattg tgtggacgcg gcctcatgga
     1561 cctttcacac gctgtggatg gttcgtcatg cgatggtgaa gggactattt tcaaacgcgg
     1621 atctcggcgt agaggtggat gcgctacgag gattacgcct cgcggtggat gccgcatcac
     1681 aacaggtgga cacccggccc ttccaccaga tgtggaagag ggcgctggga gcaagccgag
     1741 cgtggatacg tcaaggtggc agggcattgg gcaagatgat tcgtttcaaa ggaggcaggt
     1801 cgaccgctcc agaaacggtg gagcgggctg tgcaagacgt ggaacctttg gatattggcg
     1861 aggtggatga agtggcgaac cgactggggg aacagatgtg ggcggagcga ggatatctcc
     1921 gagccgtcga acgcgctttc gaaacccatt gggcccggta tcgtgcggct tcggcctcac
     1981 caacgagcta aggaatccat ccgtcgaaac gatgcctggt gcggatgctt atttgcaatg
     2041 agcgtcaaat cagcgacgac ttttccttcc ccggcatcca tcttttcatg gctcacgttg
     2101 cgttttctcg acttattggc gttatcgtcg tggcgggatt gacgagttgc agaacatcgg
     2161 aaaccatcag cgaatcgtcg gagcgagccc catcgccccg aatcagcgac tcgccagatg
     2221 catcggattc ggcacaagct ccgccgcgcc acgcgccccc ggtcgtgaca ggcaaggccg
     2281 agcttggcaa accagcgccc ctattttctc ttctcgacga agcagggaaa acggtatccc
     2341 ttactgatta tcgcgggaaa atcgtggtac tcgaatggtt caatccattc tgtcccttcg
     2401 tcaaggcttc ccatgagcga gggagtatga agccggcgat tgagaaagcg gaacgtcagg
     2461 gggcggtatg gcttgcggtc aattccgcgg gaccgggaaa gcaggggcat gaagccgtag
     2521 cgaatgcagc ggcaaagaaa cagtggaaca tgaactatcc ggtgttgatg gacgcgacgg
     2581 gcgaggtggg acgagcgtat ggtgcccaac gcacgcctca tatgttcgtc atcgattcag
     2641 cggggatact cgtatacatg ggggccatcg acaattcgcc ggatggggat cgaggttctc
     2701 ccaaagacgg ggtgctggtt tcccatgtcg aagaggcgct cgaggacatt gccatgaagc
     2761 gggcggtgcg gacggcatcg acggaagcgt acggctgcac ggtgaagtat gggggagatc
     2821 gatagcggcg cgttgttttt taggtcaatc gacgttgtga tccataagat gcttgcttcg
     2881 ggggcgcatc gttgccaaac ggggggggtg acgaaaacgt gtcgaattgg ccagatctta
     2941 ttgggagtgg ttcccatcct atgggttagc tcggtggctc gtgcgcaacc agaaccgccg
     3001 ccgcagcaca ttcccaccga tgttcatccg ccttcgcccg tgcgctggaa gttggtgatg
     3061 ggggggcttg ccacgacggc ggggttttat gcggttgcgc aaccgttttc gtatgcgtgg
     3121 ccggactctc cagggtcgcg ggatttgcga ataccggtgg ccggaccttg gatttcggtg
     3181 acgaacatcc gttgtcggga cacggacccg gattgcagtg gatttttgct gtggatgcgc
     3241 ggcattttta cggtgctcga cgggcttggc caagcggggg gattattgat ttccctcgag
     3301 ggcgccctgc tggaaaccgc ggagccaagc gtcgatccat cgccgaaacc acggcaagag
     3361 ccgagcccga aagatgagcc accgccacgg aacctattca tcggccccac tcccatgggg
     3421 gtgggtgatc gcggtcttgg aatcagtgta tccggtgcgt tctgagcagc acataccgct
     3481 cgtgggttct catggatcgg ctacggaaag ggccaacgcc gcgtaaccga caactctatc
     3541 gcgtccttcc ctatagacgt cgccgctatt tttgacggac agtcgatgca ccaatcctgt
     3601 ggaaccgaag ttgcgggcat acgcccaggc cgtcatgatc ggagcaattc cgcatggtcc
     3661 tcgtcgttcg tcatgtttga gaaagacggt ttcgatatcg ttacgttcga gttcggaaac
     3721 gatcgcttca tcgatcgttc gagcttcgtc ataggaatag aagtgagata gatccgacga
     3781 cgcgatgagc agcgcgtgtg gatcgtggcc gatgacttga aacagcagtt ttccaaggcc
     3841 ttcgagacga tcccttggca tcatggggac gatgagaggc acgagttgcg ccccgggaaa
     3901 ggcaactttg acgaacggga cgtgaacatc gagagcatgc tcgggtcgaa acagcgcgtc
     3961 atcgtattgt accaaatcgc ctttggcaac gagcgcatcg acgatcgatt tcgcaatggg
     4021 cacggttccc aacggtgtgt cgtaagcatc tgcggaaagg gtgcaaacga aagggcgccg
     4081 tccatgatga cttggcgaca ataccacgac ggtacgaacg gattggggtt gggcggcggc
     4141 ataagccatg ccggcgatcg agccagagta aacatatccg gcgtgaggcg cgacgagggc
     4201 gacgatacgg cgggcgggtc ccaaggcggg agacgatttt ccttccgcga ggaacgactc
     4261 cacggaggag cgcagttcac gggggttctg tggatagaat cgaccggcga cggtgggtcc
     4321 gaaggtggtg gtaggcgaat gagtcatggc ggcatcgggg ctgggttgtt gtcgagaaga
     4381 attccgtgaa catccagagc atagaatcgg cagggtgata agaaagcttc ggcgatgcat
     4441 gcgtacggca gtgagcaaag cgcaagcgcg gtgaggattc aagagatggg gcaacttggc
     4501 atgtggagtt ggttacggtc gtagcgcggt atccgtttcc atggcttcat tgagcacgga
     4561 tagaaaagcc cagaccactt cgggatcgaa ttgttcgccg gcgcatcggc tcaattcgtc
     4621 ggcggcttgt cgaatggtcc tcgcttttcg gtagggtcgg tcggtcgtta tggcatcgaa
     4681 cgcatcgaca acggcaatga ttcgaccgaa aagggggata ttttcccctg ataatccgaa
     4741 gggatagcca cgaccgttcc aggcttcctg atgatacaag ataccatcga gcacttcttt
     4801 gggaaggtcg acggggctca cgattcccac gccgagttgg ggatgttctt tcatcaaccg
     4861 ccattcttct tcgcacaggg ggccttgttt gagcaacacc cggtcgggga tgccgatttt
     4921 gccaatatcg tgcagcaggg cgctttgttc gagcagcatg agttgttcat tgggaagtcc
     4981 gagacaatgg ccgacagcga tcgcgaagtg ggaaacgcgc tgcacgtggg agctggttcc
     5041 cacgtcccgc gcctcgaccg cgttcgcgag agcgcgcaaa atatcgcgtt gttgtcgcaa
     5101 caatttcgcg gcttcgattg cggtgccggc gcgatccgcg acaagctgaa ccaaccgttt
     5161 ttcttcttca tcgaatacgc caatgagcag tcggttctcg aggtatacaa ccccaagagt
     5221 cgagtctctg cctcgaatcg gaacacatag aatggatgtc agtcccaacc gaatcacgct
     5281 gccatcgctg cctccaaatg cgtccacctc ggcaacgtta tcggaaagaa tgacctcccc
     5341 ggtttcggct gctgcgcgaa tgattctttt gctgacgaaa cggtcttcgg attccggaag
     5401 atagcggccc tttcggtcca atcccacacg ctgttcgagc tggcccgtcg gtcctcgaat
     5461 aaaaaggaat ccttgttccg cacgagttag ctgcacgatg cgggaaaggg cgcgatcgaa
     5521 tacttcatcg agatcgaaaa gatttccgaa atcttcgctg agtttttgcg cttcgagcat
     5581 tcgcgtatgg gcgcgggcgt tggtcaccgc caaagcagcc accgcggcta cggactcgag
     5641 aaatgcgagg taagcaggca ggccatcgcc gccgatctga atatgcttgg tggcgctgtc
     5701 gagacaaagc acaccgagaa atcggcttcg gtcgagcaag ggcaacccaa cgacggtccc
     5761 caagttccaa aaggagctat cgccttcact cgcttttcgt gagcatggga tgacgggacg
     5821 gcgtgtggcc tttacctctt cgaacaaggc ccgtgttgca tcttgatcgg taggggaata
     5881 cgcaccgctg tctttgttgt cgcaagagac cacggcttca tagcctcccg ccccttcatc
     5941 gagcagcagc acgcctcttt ccattttcag cacgctcagc acttctcgca cgacgagcgg
     6001 cagcaattct tgtaactcgt gggtatcggt cagacgtcga gtcagccgaa caagggcatg
     6061 aaaacgccct tcggcagagt tatcctctcc gcaaccgcta tgcgcattgg agtcgccgca
     6121 cggttgcatg ggacgactct caggctcttt cacaccagtc tccttttcgc aaaaccaggc
     6181 ggttcacatc gtgcggtatt ctatcgcacg acgattgcag gtcgaagggt ggaattcggc
     6241 aagcgcgcgg gaatgtattg tttcggcatt cgcatcgaaa aacacactca ctggcccctg
     6301 gattccggct agggtaagct agagcaaccg agagtgctcg accgaaaggc acttgccgac
     6361 ggcgctcacg cagcgaatca cccatgggct ttttcgaaac cctcactggc aaaaagacct
     6421 tctgttctgt ttgtggagta cccgttggct cgtcatgttt gcgcgatgct acgggtgagc
     6481 cgctttgcag tgaagcctgt cgcgacgcga tgcaacgttt ttccaatgcg cctcctgcga
     6541 tggaaaatgc gccggaggcg gcggagctaa ccggcaaagc gcccgcgtcg ccgagtcctt
     6601 ttccccatcc ggaattggtt gttttcgtgt ttcgtacgaa tacggcgacg gcgttttttt
     6661 cgcttcaaga cgaactcatt gtggcgagtg gagagccgaa gcttcaggtg caaatgtggg
     6721 aaggccaagg ggtgtcgctg gtagtcgtca atggccctgc tggttccatc cagacgtatc
     6781 ggcgaacgtt gcgaaccaat ctcgggcgtc ttgcgaaagc ggcgaagtgt cggcccgagc
     6841 ggattcgtcc ggatgttacc tactcgaatg tgagcgaaat gggcgagtac cacccggatg
     6901 acaaggcgat tcagagggta tgcgacaaaa ttcgaagagg agcttttcgt cgccggtcat
     6961 cgactgtctt gacatcacct gcggagtcga cgtcaccgcc aacgcctcga cctcgcaccg
     7021 catcggatca atcgctgctg agaaacgcga tcttccaaat cgacaagcat gtcacgttcg
     7081 atgcgaaagg cgaaccgcaa gtccaactcg agggcgtgcg tgaagcagct cgtttgctcg
     7141 agcaactcgc tgaccgccat ccaagcgatc cagaaccggc aacactcgaa acctccgccg
     7201 tcctactcga tacgcaacat gctcgttgca gcacatgcat cgagacattg gtccaacgtt
     7261 ttcccgacca tgccgaagcg cgcatgatcg ccatgacccg caaatcgata ttcgccttcc
     7321 ccccttacga tgtcggcgcg tcacttcccg actttttgcg caagcgcatt cgtggtgcca
     7381 cgctgcaggt cgtgcgacag ggttgtttgg cgcacgccgt gctgttcgtc gaatccagca
     7441 ccgcgtatcc cacatctccc gcgcctgtgg cctatcccct ttacgttcaa tccgcaggga
     7501 tacccgtttt cggcgtggcg gtcgtgtttg ccgacggttc cagggtcgag actacggtcg
     7561 cgggatttga agagcacgac ggccactgga gcctggcgcc gaaagcgtgc tatctattgt
     7621 gcgcggatac gctgcctgtc gttacgctgg tgccgtggga cgcgggctcc ccgaagcgtg
     7681 cgaacgagcc gctttccgta gcgtatgcgg tgttcgaccc gacgagtcgc gaggctttcg
     7741 ttggttgcga ggctacgtat cgagggctac agccgcgggc atttccgctc cacgaactca
     7801 gtagtgctct ccaacatttt cgggataaaa tatcatcgat cgggaaatag cataaaaaac
     7861 gccgagtttc gctcgaccaa gtcctgtatt caaccacccc tcatatccta cttgacctct
     7921 attaaaacca aggtagtttt gcgccgtaca tataacgaca atcatcgttt atggagagga
     7981 ttcatgaaag cgtattggtt aggttggatt ggattgggtg cgttactcgc tttgacgggg
     8041 gccaaaggcg atggctgcca accggcgcca caacaagggg aagtatgtgg ggtgggagat
     8101 gcctgcgcgg ttgggctgac gtgcgttcat tactacggta ttgcggggcc ttcggggcct
     8161 gagtttcgca gttgcgagat tccttgcacg caagggtcgg attgccccaa ggatcaagct
     8221 tgcacgctga tcgcggatgg gccgggccat gtgtgccgac cgtcctctga cgcgccatgt
     8281 gatccttcgg tggagcagag ttgtggggcg aacgaggtat gcgttgcgga tacgctcacg
     8341 gaccaaggtt tctgccatct ggccgggcat gggcaaagcg tgggcgcggg gtatgcctgc
     8401 ggtggttcga taggaaatac gtgtgcgcaa gggttgtact gcaaaggcct cgcccatgac
     8461 gtacacgggg gaacaggcgt ctgtgcccag atgacttgtg atgattggag gaccgagttc
     8521 gacaacttct tggcgtccat gcaaggctgt gtcacggcgg aagactgcgc ggccgtgagt
     8581 ggaacgagtt gtggttgcac caggaacgtc gtggtcaata agaacgggga ctacgaaggg
     8641 ttcgtagcgt ttgtgcagca aatgaacgat gcgggttgtg ggcgaaggac gccctgcgat
     8701 tgccccgcgg cgcaaggatt tgcttgtgtc gatggcattt gcggttggaa ttatcagtaa
     8761 tcgaggcggt tcgacgttct gtcgaacgca agttcagcac cccatttccc tgagaatcat
     8821 ggccgaagat ttcgcgattg ccgcgaaaca cgagcagctt agtgacgacc tcctgtcccg
     8881 ggcaagccgc gtggggcagg cgcgagggca accggcgtga tgtcgcacgg tggtgcgctt
     8941 gcccacgcgt tgcgaatacg gggtgatgag catccttgtt tgctcgaacg caagctacag
     9001 gcgtggaaga tagcgcctgt cagcgagggg aagtggggag attccgggag tttcctcacg
     9061 ccgcgtttca gcagcgctgc attttagaaa tgggtgaacg tgtagccgac acttgcgccg
     9121 acaagcatgg agtcaccggc atgtccgagg tcgagcatgg aaaatgtccc ccgcacgtcg
     9181 agatcgccgg gcttgaaacc gacactgagt cccgcaccga agtctgtatc cgaggcgctc
     9241 gcggagactc ccatgacctc cactttcgcg gttaccatgt tcagcccaag ttcccctccg
     9301 acgtacgcat tctcgaagct gccatcgaaa aagtagcgaa ctcccgccca gatcggaatg
     9361 tccgacatcg agcagcagtc gtttttctcg agaccataga gatagccggc gcgaccggtc
     9421 acctcgagtc ctggattcac gggcacctcg agtttcagaa gtgcaccgag catgggaccg
     9481 acgccatcac cgagattacc gatcggcatg aacaccgcgg cgtcaccacc taacccgatc
     9541 cccggcgtat cactatcctg cgcaaatgcg ctcgccgcgg taaaggaaat gaccagcccc
     9601 aacgccatcg atacatgttt tcgaagggta tgatccatta cccggtcaga cagcacctca
     9661 atgggaagag gcttggaa
//
LOCUS       DAOSOR010000173         9628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_7060_length_9628_cov_4.430690, whole genome shotgun sequence.
ACCESSION   DAOSOR010000173 DAOSOR010000000
VERSION     DAOSOR010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9628
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7060_length_9628_cov_4.430690"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PK710_11040"
     CDS             <1..353
                     /locus_tag="PK710_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOT10300.1"
                     /translation="GITTKALLQAIDDELEQVRRAPPTEIEVQRAKANLEASLVFHIE
                     QIGHRADMFNLYAQHTGTADYFAQDIARYRALTASSLHRAAQSYLPRSDRLVVFVEPT
                     PGAPRAGRVKGGKP"
     gene            350..1891
                     /locus_tag="PK710_11045"
     CDS             350..1891
                     /locus_tag="PK710_11045"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOT10301.1"
                     /translation="MRHPSLVNTLLTIGFITVFYGCASSSSQTPMPTAPPIKPTSVEP
                     AEAQWQPTPDAAFRHQAPAPGAEAPWEAPIPLETRLSNGMRVWFIARPALPIVAIQVT
                     SPRGADQQPSPGMGAFLGAMLEQGTTTRNALQVSDAYLDIGASHGAWVDWDSTNAWIQ
                     VLPQHLDRGIEILADVFRNPSFAAEEVQRVRSQTIASIRQQNDQPRVLVDNAIARTLF
                     EGSPYGESLLGTESSVASITSQSLKALYRATVAPAESFVVVVGNTTQSEVTASLEKAF
                     GNWKSVAPARQRLRPASRQSKKIRVIDRKGSAQSTLAVTTVGVSRTTKDFDAVLIGNT
                     ILGGMFSSRLNLNLREKHAFTYGAYSMFDMRRGPGPFVATAAVDTPNTGAAIKEILNE
                     INTFRSSRVTEEELRLALGRQVKSLPGRFQSATTTARSIAALGVFGLANDEYRQRPGR
                     LERVTPAQVQEMASNRLNPNELQIIVAGDLSVIRPQLMALAYGVIEIIDPSTSKVLET
                     IADSP"
     gene            complement(1928..3751)
                     /locus_tag="PK710_11050"
     CDS             complement(1928..3751)
                     /locus_tag="PK710_11050"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT10302.1"
                     /translation="MNLFPGTMIAGMYRVDRRIGVGGMGEVWAGVRLSDNVGVAIKVL
                     LAHATDANEVQARFRREAQVLVRIRSPYVAQLIDFLTEDDGFILVMELIPGETLHAVL
                     AYDQALTIEATLDIVSDVLHGLRDLHAANIVHRDLKPGNIVLKPRPGQRPRATLIDFG
                     VSRIISAPEDEEEITAITRGDRVLGTLEYMAPEQILGSRTVTGTADLYAVGAMMYRAI
                     CGHHVFGDLQEGHLAVAKLNQDAPRMQTGRTDEIAERTQGLVARLLSRRLRDRFKSAE
                     EALVEVASIRSLYLDRNSLPQGELDDEPTFEKPWQRMTEGSARTSAPAVAVDYRAATP
                     LADPGAARPRMVEPEVVQPVASEPANAVSASYATGAPSATVGTSAHLMARPSPIPAPS
                     SQPSFEPTQQPPSVHLHASSSMQPISAARSAAPLGTKPHPRRPKEDNRIRTLFVALVL
                     FMLGSVGGGLFGVSYARGGLAFRILHRDQSGSALPSVSPEVAVPPALSMEPILIEEQR
                     IDSMEASHEQEATDAREESEPEPEPSPVLPHQIPPALSSGTESAEPSAGAVAATSPSG
                     PSSSSRPPTRRPPVVPPKNTAPKPMPTAPPAETKALLPEEP"
     gene            3944..4699
                     /locus_tag="PK710_11055"
     CDS             3944..4699
                     /locus_tag="PK710_11055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOT10303.1"
                     /translation="MNLVLLEPQDQRQDGCFELRGPRASHIREVLRAVTGQRLRVGML
                     EGSPGVGEVLEIDDERVCLRCEFDTLRPARPPIDLVLAVPRPKSLRKLLPEITAFGVD
                     RIVLLRTWRVEKPFLSSSILQPAVYRPLLHEGMMQGRHTREPHILIEPLFNPFVQDRL
                     DTYFSSAKHKIVAHPGAKRSLAQLEIEPTDRVVAVIGPEGGLVAYELDQLERHGFQTM
                     TMGPAPLRVETACVALMAQIELLRQRKRRCDEA"
     gene            complement(4704..5465)
                     /locus_tag="PK710_11060"
     CDS             complement(4704..5465)
                     /locus_tag="PK710_11060"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOT10304.1"
                     /translation="MNQVNAARLRDARQDVVEVFRRVHARGWIAATDGNASVLIDDHI
                     VATPTGIHKGYMQPNDLIIVDRHGRVVEGERRPSSEIEMHLAVYEQRPDVRAVLHAHP
                     THCIALSLAGVRLDACLLPEVVLTFGSIPTVPYTTPTTQEVPDEVRRWVRDYDALLLE
                     RHGSLTMGADIFDAYNKLERMEHVAEIVFRARLMGPVQSLTCEQISKLQHVVRALGLP
                     DRPLLDSPCSRGNNESSGQDIDALVHRVKAQLSRI"
     gene            complement(5462..6148)
                     /locus_tag="PK710_11065"
     CDS             complement(5462..6148)
                     /locus_tag="PK710_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10305.1"
                     /translation="MRTRLFFALLLISSGCSKQPMVVQEPVAEPVPEKPKLMMYGEIG
                     VLDEAQVRSTFERVWRGPMTDCQKQGGDMISGDTIVRLRVNHGGVVKWAYLKETNLGD
                     RNVEKCILDAVRNTTWPTPEGGEDGIAEQELPFADYADRPPVEWPADKAHSAVAQHAS
                     SLSSCRKAVAGSFVATIIVNKNGSVASVGIQQPDETADDAADCMVGVLRKITFPKTGS
                     WPAKVTFDIP"
     gene            complement(6169..7311)
                     /locus_tag="PK710_11070"
     CDS             complement(6169..7311)
                     /locus_tag="PK710_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT10306.1"
                     /translation="MEFDLTEEQKMIRDTARDFATREVEPKAALLDEKGIWPTEFVQK
                     MGELGFMGVAIPDEWGGSGMDTVSYVLAMEEISRACASTGVIMSVNNSLFCDPVLKFG
                     TEQQKREYLVPCASGAKLGSFALTEPMSGSDAQTMVTSVETQGDDLILNGSKNFITCG
                     NHADFVIVFAVSGRDGKRLKHHALLFPTDLPGVTKSPPDEKLGIHAAHSCTLYFENVK
                     LSKEHLLGPEHKGFKVAMTTLDGGRIGIAAQALGIARASLEKSIVYAKERKAFGGPIA
                     NLQAIQFMLADMATQLDAARLLTLRAAWLKDRGIRHSSESAMAKLLASEAATFITHKG
                     IQIHGGYGYTREYGLERHYRDARITEIYEGTSEIQRLVIAAHELRD"
     gene            7594..8838
                     /locus_tag="PK710_11075"
     CDS             7594..8838
                     /locus_tag="PK710_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10307.1"
                     /translation="MMKTGTKRSVILSSLPLLALGAMMIPGSACDKIEEVQGAVCCDA
                     DDFQVGGTISAKIGGGAQAQVAVQAIADLAGVASVMVDDLTVACRNIAQDLDAPEDKQ
                     KEADAKENKRDKVKAWCELAVAQIGSFKGSAKLTIKVDPPKCEASVSAKASCQGSCSA
                     EASCDIKANPPTCEGGSLEVACKGECTAKAGASLKCEGSCGGECKGSCEAQGGVKCAG
                     KCEGTCEGDGSAGNEGIQADGTCKGTCKGTCEVTAPGASCTGSCKGECKGSCTGSAEA
                     SVKCDGECKADFEPLKCTGGELKGGCQVDAKCEANCDASVKAKAECKPPAVVIDFQAA
                     ADAKLNKLKATLEANLGFIYGAEAKVKGLVDISGTLSGNVSGLVEIKAACLPAVAKAI
                     DGAVTDIGVSVQVTGSIVGSIK"
     gene            8922..9389
                     /locus_tag="PK710_11080"
     CDS             8922..9389
                     /locus_tag="PK710_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10308.1"
                     /translation="MRFVHGAFLSTAIVCLTASCYSTKVNTPQDEEDVFAGTSLDPNA
                     APVNEAPPPKNAKEPDREFIKDMLRRSADQAAKCNVEANAGPRSNVYVNVTFTRNGRV
                     SKIDVAPPHRDTPIGDCVQRAFEGIFVTGWQSNESVTMERIVDFNKKAQDPTP"
     gene            9417..>9628
                     /locus_tag="PK710_11085"
     CDS             9417..>9628
                     /locus_tag="PK710_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10309.1"
                     /translation="MVLGNYPTSIEPLHPTFAHRSHLWIKHDDATHPVYGGNKVRKLE
                     GLLRDARQIQARRIVTFGAAGSHHVL"
BASE COUNT         2123 a         2857 c         2672 g         1976 t
ORIGIN      
        1 ccggcatcac gacaaaagcc ttgctccaag ccatcgacga cgaactcgaa caggtacgcc
       61 gcgctccccc caccgaaata gaagttcaac gcgcgaaagc caacctcgaa gcctctttgg
      121 tgtttcacat cgaacaaatc ggacatcgcg cggatatgtt caacctctac gcgcaacaca
      181 ccggcacggc ggattacttc gcccaagaca ttgcaaggta ccgtgctctc accgcttcgt
      241 cgcttcatcg ggcagcacaa tcgtacttac ctcgctccga tcgcttggtt gtcttcgtgg
      301 agccaactcc tggcgcaccg cgtgcgggtc gtgtgaaagg agggaagcca tgagacaccc
      361 cagcctggtc aacacacttc taactattgg atttataaca gtattttatg ggtgtgcctc
      421 atcttcttcg caaacgccta tgccaaccgc tccacccatc aagccgacct ctgtcgaacc
      481 ggccgaagca caatggcaac ccacccctga cgctgccttt cgacatcagg cgcctgcccc
      541 tggtgcggaa gcgccctggg aggctcccat tcccctggaa acacgccttt ccaacgggat
      601 gcgcgtgtgg ttcatcgccc gccctgctct tcccatcgtc gccattcagg tcacatcacc
      661 caggggagcg gatcaacagc cttctcccgg tatgggtgcc tttttgggag ccatgctcga
      721 gcaagggacg acaactcgaa atgcccttca agtttcggac gcttacctag acattggcgc
      781 ttctcatgga gcttgggtgg attgggatag caccaatgca tggattcagg tgctccccca
      841 acatctggac cgagggatcg agattttggc ggacgtattt cgtaatcctt cgttcgctgc
      901 cgaggaagta caacgggtaa gatcacaaac catcgcttcg attcgtcaac aaaacgatca
      961 gcctcgagtt ctggtcgaca acgcgatagc gcgaacgttg ttcgaaggaa gtccgtatgg
     1021 tgaatcgttg ctaggcacgg aatcgtctgt cgcttccatc acgagccaaa gcctcaaagc
     1081 gttgtatcgc gccaccgtgg cgcccgcgga atctttcgtg gtcgtcgtgg gaaacactac
     1141 ccaaagcgag gtcaccgcct ccttggaaaa agcttttgga aactggaaga gcgtcgcacc
     1201 agcacgacaa cggcttcggc ctgcctcgcg acaatccaaa aaaatccgcg tgatcgaccg
     1261 caaaggatcg gcacaatcca cccttgccgt gacgacggtt ggggtttcac gaacgaccaa
     1321 ggacttcgat gcggtcctca tcggaaatac catccttggg ggcatgttct cgagccgact
     1381 gaacctgaat ctacgagaga aacatgcgtt cacctacggc gcttacagca tgttcgacat
     1441 gcgtcgtggg ccgggaccgt ttgtcgccac ggcagccgtg gacacgccga atacaggcgc
     1501 ggccatcaag gaaatactca atgaaatcaa tacctttcgt tcctctcgcg tcaccgagga
     1561 ggaacttcgc cttgccttgg gccgacaagt caaaagcctt cctggccgct tccaatccgc
     1621 aacgaccacg gcacgatcga tcgccgctct cggcgtgttc ggccttgcga acgatgaata
     1681 tcgtcaaaga cccggacgtc tcgaacgtgt cacgcctgct caagtacaag agatggcgtc
     1741 caaccggctg aatccaaatg agttgcagat cattgtggca ggagatctat cggtcatccg
     1801 ccctcagctc atggcgcttg cctacggtgt catcgaaatc atagacccat cgacatcgaa
     1861 agtcctcgag acgattgcag actcacccta ggcgcgagcc gtcgccgata cggccttggt
     1921 tacccaccta cggttcctcg ggcaacaacg ccttggtttc cgcggggggc gccgttggca
     1981 tcggtttggg agccgtattt ttggggggca caacgggtgg gcgacgagtt ggcggcctgg
     2041 aactggatga aggaccagaa ggagacgtgg cggccacggc tcccgcggaa ggttccgcgc
     2101 tctcggtgcc cgaagataga gccggaggta tttgatgcgg tagcaccggg ctcggctcgg
     2161 gctcgggctc cgactcttcc cgcgcgtcgg ttgcctcctg ttcgtgcgaa gcttccatcg
     2221 agtctatccg ttgctcctca atgagaattg gctccatcga cagcgcgggt gggactgcca
     2281 cctcgggcga gacggaagga agagcactcc cgctttggtc acgatgcaag atccggaatg
     2341 ctagccctcc acgggcatac gatacgccaa acaatccacc acccaccgag ccgagcatga
     2401 acaacacgag cgcaacgaac agtgttcgaa tgcgattgtc ttcctttggc cgacgcggat
     2461 ggggcttcgt tccgagcggt gccgcggatc gagccgctga tatcggttgc atggacgaac
     2521 tggcgtgcag atgaacggag ggaggctgct gcgttggttc gaaagaaggt tgggatgacg
     2581 gtgcagggat tgggcttggc cgcgccatga gatgagccga ggttcctacc gtcgccgacg
     2641 gagcaccggt tgcgtaggag gcagataccg cattggctgg ttccgaagct accggctgca
     2701 cgacctccgg ctcgaccatc cgtggccgcg cggcgccagg atcggcaagg ggagtcgcgg
     2761 cgcgataatc cacagccacc gccggcgcgc tggttctcgc ggacccctcg gtcattcgct
     2821 gccaaggctt ctcaaacgtg ggttcgtcat ccaactcgcc ttgcggcaat gagttccgat
     2881 ccagatacag cgaacggatg gaagcgactt ccaccaaagc ttcttcggca cttttgaatc
     2941 gatcccgcaa ccgccgagat agcaagcgag cgaccaaacc ttgcgttcgc tccgctatct
     3001 cgtccgttcg acccgtctgc attcgtggcg cgtcttgatt caacttcgca accgccaaat
     3061 gaccctcttg cagatcgcca aacacatgat gcccgcagat ggcgcgatac atcatcgcgc
     3121 ctaccgcata cagatccgcg gttcctgtca cggttcgaga ccccaggatt tgctcgggag
     3181 ccatgtattc tagggttccg agtacacgat ccccgcgcgt gatcgccgtg atttcttctt
     3241 cgtcttccgg cgctgaaatg atgcggctca cgccaaagtc gatcagagta gcgcgtgggc
     3301 gctgtcccgg acgtggtttc agaacgatgt tgccgggctt caaatcccga tgaacgatat
     3361 tggccgcatg caaatctcga agtccatgaa ggacatctga tacgatatcg agcgtagctt
     3421 cgatcgtcag tgcttggtcg taggccaaca cggcatgcaa cgtctcgccc ggaatgagtt
     3481 ccatcaccag gatgaaaccg tcatcttccg taagaaaatc aattagttgc gccacatacg
     3541 gactacggat gcggaccagg acttgcgcct ctcgacgaaa gcgggcttgc acctcgttcg
     3601 catcggttgc gtgagcaagc aataccttga tcgccacgcc gacgttgtct gaaaggcgaa
     3661 caccggccca cacctccccc atcccgccaa caccaatgcg acggtccacc cggtacatcc
     3721 cggcaatcat ggttcccgga aacaaattca cgtcgacgca ccatagtacg atacggcacc
     3781 gacgaggcga acttctcttc gcctcggggg gggtgattct acacaaaccg ccccaaagca
     3841 gccttgcctt gggcctatcg tgtggctcga aggtgatgtc aagatgctcc ggcagcctgg
     3901 ggcatgacat cgacaacaac ccgtgggatg aagggatagt cctatgaatc tcgtgctgct
     3961 ggaacctcag gatcaacggc aagacggttg tttcgagtta cgcggcccgc gggcctcgca
     4021 tattcgcgaa gtcctccgtg ccgtgactgg acagcggctc cgcgtgggca tgctcgaagg
     4081 gtcccctggg gtgggcgaag tgctcgaaat cgatgacgaa cgagtatgtt tacgctgcga
     4141 attcgacacc ctgcgaccgg ctcgcccccc catcgacttg gtgctcgccg tgccaagacc
     4201 caaatccctt agaaagctgc ttcccgaaat caccgctttc ggcgtggatc gaatcgtatt
     4261 gttacggacc tggcgagtcg aaaagccctt tctcagttcc tcgatcctcc aacctgcggt
     4321 gtatcgccct ttgctgcacg aaggcatgat gcaaggtcgt cacacccgag agccccacat
     4381 cctcatcgaa ccgctgttca atcctttcgt ccaagaccgt ctcgatacct atttttcaag
     4441 tgccaaacac aaaattgtgg cacatcccgg agcaaaacga agcctggccc agctcgagat
     4501 cgagccaacc gatcgcgtgg tagcggtgat cggtccggaa ggaggattgg tggcttacga
     4561 actcgatcaa ttggaaagac acggatttca aacgatgacc atgggccccg caccgctgcg
     4621 tgtcgaaacg gcttgtgtgg ccttgatggc gcaaatcgaa ctccttcgac aaaggaagcg
     4681 tcgttgcgac gaggcatgaa ggttcagatt ctggagagtt gcgctttcac acgatgcacc
     4741 aacgcatcga tgtcttgccc cgacgattcg ttatttccgc gggagcatgg cgaatcaagc
     4801 agcggacggt cgggcagccc caacgctcgg accacgtgtt gcaatttcga aatctgttcg
     4861 caggtgagag actgcaccgg acccatcaac cgggcgcgaa acacgatttc cgccacatgc
     4921 tccatgcgtt ccaacttgtt gtaggcatcg aagatatccg cgcccatcgt cagacttcca
     4981 tgacgttcga gaagcagagc gtcgtagtct cgtacccacc gacgtacttc atcaggaacc
     5041 tcttgggtgg tgggtgtcgt ataggggacc gtaggaatcg aaccaaaggt gagcacgact
     5101 tcagggagca agcatgcatc cagtcgtacc cccgcgagcg acaacgcgat gcaatgggtc
     5161 ggatgagcgt gaagcacggc gcgaacatcc ggacgctgtt catacacggc aagatgcatc
     5221 tcgatctcgc tcgatggtcg acgctcacct tcgacgacgc gtccgtgccg atcgacgatg
     5281 atcaggtcgt tcggttgcat atagccttta tgaattcccg ttggggttgc cacgatgtga
     5341 tcatcgatca gcaccgaagc gttgccatcg gtggcggcaa tccatccacg cgcatgaaca
     5401 cgacgaaata cctcgacgac atcttgccgt gcgtcacgta gccgagcagc gttcacttgg
     5461 ttcacgggat atcgaaagtg accttggccg gccagcttcc ggttttgggg aacgtgattt
     5521 tccgaaggac accgaccata caatccgcgg catcatcggc cgtttcatcg ggttgttgga
     5581 tgcccacgga agccacggaa ccgtttttgt tcacgatgat cgtcgccaca aacgaacccg
     5641 cgacggcttt gcggcaagag gaaagggatg aggcatgttg cgccacagcg gaatgagctt
     5701 tgtcagcggg ccattcgacc ggaggccggt cggcatagtc ggcaaacggt agttcttgct
     5761 ccgcaatccc gtcttccccg ccttctgggg tcggccatgt cgtgttacgc acggcatcga
     5821 gaatacattt ttcgacgttg cgatcgccca ggttggtttc cttgagataa gcccacttga
     5881 cgacgccacc atggttgaca cgcaaacgca cgattgtgtc gccagaaatc atatcgccgc
     5941 cctgtttttg gcagtcggtc atgggtccac gccacacccg ttcgaacgtc gaacggactt
     6001 gcgcttcatc gagcacgcca atctcaccgt acatcatgag cttgggcttt tctggaaccg
     6061 gttcggcaac cggctcttgc accaccatcg gttgtttcga acaccctgat gaaatcagga
     6121 gaagagcaaa aaatagacgc gttcgcatga gagtatcgtg gcggaatatc aatcgcgaag
     6181 ttcgtgagcg gcgatgacga gacgttgaat ctcgctcgtt ccttcataaa tctcggtgat
     6241 acgggcgtct cggtaatgtc gttcaaggcc gtattcacgg gtatatccgt aaccgccgtg
     6301 aatttggatt cctttatgag tgatgaaggt agctgcttca ctcgccagta gttttgccat
     6361 ggccgattcg ctcgaatgac gaatacctcg gtctttcaac catgcggccc taagcgtgag
     6421 caaacgcgcg gcatcgagtt gcgtggccat atcggccaac atgaactgaa tggcttgcaa
     6481 gttcgcgatc ggtccgccga aagccttgcg ctctttggcg tagacaatgg atttttcgag
     6541 ggatgcgcgc gcgatgccga gcgcttgcgc ggcaataccg atacgcccgc catcgagcgt
     6601 ggtcatggca actttgaacc ccttatgttc gggaccgagc aggtgttctt tcgacagttt
     6661 cacattctcg aagtacaggg tgcaagaatg cgccgcgtgg atgccaagct tttcgtcggg
     6721 cggagacttg gtgacgcccg gcaaatccgt cggaaaaagc aaagcgtgat gcttgagtct
     6781 ctttccgtcg cgtccactga ctgcgaaaac gatgacaaaa tccgcgtgat ttccacaggt
     6841 tataaaattc ttcgaaccgt tgagaatgag gtcgtcgccc tgcgtttcga cactcgtgac
     6901 catggtctgg gcgtcggagc cgctcatggg ttcggtaagg gcgaagctgc caagtttggc
     6961 accggaagca caaggaacga ggtattcgcg cttttgttgt tccgttccga atttcagaac
     7021 cggatcgcag aacagtgaat tgttgacgct catgatgacg ccggtggaag cgcaggcgcg
     7081 gctgatttct tccatcgcca gcacgtagct gacggtatcc atgcccgatc cgccccattc
     7141 gtcgggaatg gcaaccccca tgaagccgag ttctcccatt ttttgaacga actcggtagg
     7201 ccagatgccc ttttcatcga gcaaggcggc tttgggttcc acttcccgcg tggcgaaatc
     7261 ccgagccgta tcacgaatca ttttttgttc ttcggtgaga tcgaattcca tgttctgttc
     7321 cctggttgcc tactgtttcg tccgcaggtc gctcatggag gggcgtcgtc aggtctatta
     7381 ccgatgctgg caggtggagg caacccggat aaaatcggct cagggtaggc attcgttcag
     7441 tttcgggtgg ctccccacga aaaacgtcgt gacagacccc caaagacgcg gtatccctag
     7501 cgtgttattt catggaggat gggctgtgcg tcatcgagcg gcccaaggcc cgccggaagc
     7561 ggcggctggg atgagagtga aaaaggagtg cttatgatga aaaccggtac caagcgaagc
     7621 gtgattctat cgagcctgcc cttgcttgcc ctcggggcga tgatgatccc ggggtcggcc
     7681 tgcgataaga ttgaagaggt acaaggggct gtctgctgcg atgcggacga ttttcaagtt
     7741 ggcggaacca tctcggcaaa aatcggcggc ggtgcccaag cccaggtcgc cgttcaggcc
     7801 atcgcagacc tcgctggcgt tgcaagcgtc atggtcgacg acctcacggt agcttgccga
     7861 aacatcgcgc aagatctcga cgcaccggaa gacaagcaaa aggaagcgga cgccaaggaa
     7921 aacaagcgtg acaaggtcaa ggcgtggtgc gagctcgcgg ttgcccaaat tggaagcttc
     7981 aaaggaagcg ccaagctcac gatcaaggtc gatccgccca agtgcgaggc ttccgtctcc
     8041 gcgaaagcat cctgccaggg tagctgttcg gccgaggcaa gctgcgatat caaggccaat
     8101 cctcccacct gcgaaggtgg tagcctcgaa gtcgcttgca agggcgaatg caccgcgaaa
     8161 gcgggcgctt ctctcaagtg cgaaggctcc tgcggcggcg aatgcaaagg ctcctgcgaa
     8221 gcccagggcg gcgtcaagtg cgctggcaag tgcgaaggca cttgcgaagg tgacggttcc
     8281 gccggcaacg agggtatcca ggccgacggc acctgcaagg gaacttgcaa aggcacgtgc
     8341 gaagtcaccg cccctggtgc ctcctgcaca ggctcctgca agggcgagtg caagggctct
     8401 tgcacgggtt cggcggaagc ctccgtcaag tgcgatggcg agtgcaaagc cgacttcgag
     8461 cctctcaaat gcaccggcgg cgaactcaag ggcggttgcc aggtcgatgc caagtgcgaa
     8521 gcaaactgcg acgccagcgt caaggccaaa gccgaatgca aacccccggc cgtcgtgatc
     8581 gatttccagg ccgccgccga cgccaagctc aacaaactca aggctaccct cgaggcgaac
     8641 cttgggttca tctacggcgc ggaagccaag gtcaagggtc ttgtcgatat ctccggaacc
     8701 ctgtccggaa acgtcagcgg actcgttgaa atcaaggccg cctgccttcc cgctgtggcc
     8761 aaggccatcg acggcgcggt taccgatatc ggcgtgtccg tccaggtcac cggaagcatc
     8821 gtcggaagca tcaagtagcc catcctgccc cctcgtctcc ctctcgcaac ataacccctc
     8881 gcttctttcc ccttcgctca tcctcaggta aactgtagcc catgcgattc gtccatggcg
     8941 ctttcctgtc gacagctatc gtctgtctca ccgcaagctg ttactccacc aaagtcaaca
     9001 ccccacaaga cgaggaagat gtgtttgccg gcacatcgct cgatccgaac gcggctccgg
     9061 tcaacgaagc acctccgccc aaaaacgcaa aagaaccaga ccgggaattc atcaaagaca
     9121 tgcttcgcag aagtgccgat caggccgcga aatgcaatgt agaagccaat gcgggccctc
     9181 gcagcaacgt ctacgtcaat gtcaccttca cacgcaacgg tcgtgtctcc aaaatcgatg
     9241 tggctccccc ccatcgcgac actcccatcg gcgattgtgt tcaacgagcc ttcgaaggca
     9301 ttttcgtcac gggctggcaa agcaatgaat cagtcacgat ggagcgcatc gtcgacttca
     9361 ataaaaaagc acaagatcca actccttagt ctttccacgc cgctcgttgt catctcgtgg
     9421 tactcggcaa ctatcccacc tccatcgaac ctcttcaccc cactttcgct catcgctccc
     9481 atttatggat caaacacgat gatgccaccc accccgtcta cggaggaaac aaagtccgaa
     9541 aactcgaagg attgttgagg gatgcaaggc aaatccaagc acgccgtatc gttaccttcg
     9601 gagccgcggg tagccatcat gtgctaga
//
LOCUS       DAOSOR010000174         9623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_7067_length_9623_cov_4.627090, whole genome shotgun sequence.
ACCESSION   DAOSOR010000174 DAOSOR010000000
VERSION     DAOSOR010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9623
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7067_length_9623_cov_4.627090"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /locus_tag="PK710_11090"
     CDS             <1..1238
                     /locus_tag="PK710_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10310.1"
                     /translation="GALRWELLAPGLLVNAGLDPGENSLFVKAGVDAHLSVLDRMSEV
                     EEALARGGADAKGVDVAVIPMPEFIAAYERLRALEPRVFFVVGWSRGREALYAKQGSL
                     TNLPEGELTLRAEPGSSQAFLGLFSLQLAGAPPTRLRFELPSDKTPPDFEALSLMDIP
                     KVDAASGKLQLTSADAPMMVPIVAVAQAGFVDTHRAALTAWVRVWLEGEDRIRADAAT
                     SAREISKQGGAPDPLVLINRLGPLSWANLADNARWAGLSGRGALTTESLFQRGWEVWK
                     AAGLLSTPLPEAPASSPTIISALVRQLGTDQGTEKFNKNSDLPRGSHNDVLLRFAFSA
                     AEVDEDALAGEIGFLAGLFDRSTLRVAVRGTAAIHKAKAAKAVAAAIGRFGLAENRLQ
                     IASQPARTASATIEVLGAR"
     gene            1500..2228
                     /locus_tag="PK710_11095"
     CDS             1500..2228
                     /locus_tag="PK710_11095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOT10311.1"
                     /translation="MNENPSQRGLSSLLRVVKPYAKDLIFRSVVPNEDNRWLFELAFD
                     THWSAREAYEWARRSLIATPLFLSKCAVHGERISVDRIPYITACPRIELGSDIRISGE
                     IAIKGNSRRSPVLKIGNGVFLGHWTTFAVAERIEVGDFVAIGGNTYIADTEGHSHYNP
                     RQPIWTVPASDEDIEPVIIEDNVQISRDCMILKGVRIGARSVIGAGSVVRSNIPPDSV
                     VMGNPARVIKRMTPPEAEKADSSS"
     gene            2434..3672
                     /locus_tag="PK710_11100"
     CDS             2434..3672
                     /locus_tag="PK710_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HOT10312.1"
                     /translation="MNLDTIDIKAVARDFGTPFYLYDLDSALAHLHALQAGLPDYVDV
                     YFAMKANSNPDVLRAWLGHVAGLDVSSAGEIEMAVAAGHSPSAMSFAGPGKTEQELRV
                     AIERQVRLLSIESPTELRRASKVATELGKTATIAIRINPQFTPKEFTMKMGGTPSQFG
                     TAEEDAPALLEEAKTTKGVRLDGIHIFSGTQCLEIKGIIDNIRQTLAIASRLSEEHDL
                     RPSFVNLGGGFGVPYFAGQEAIDVHALAREVGELLRQAPTEHPRLRDTRFILELGRFL
                     IGTFGIYVARVVDVKQTRGKRFVVLDGGMHHCFPATGFFGQLVKKNYPVVNLSRADGE
                     PMAQELVGPLCTPMDSMARNITLPRLEIDDLVGILSAGAYCYAASPLLFLGHETPPEI
                     VVYEGAYKLSRARKAPTQLV"
     gene            3700..3960
                     /locus_tag="PK710_11105"
     CDS             3700..3960
                     /locus_tag="PK710_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOT10313.1"
                     /translation="MNNIREQIRTFILTTFMEGADADSLQDDTSLERAHIVDSAGMLE
                     IILFIEENYGFSVDNDDATPENFDTIDAMTSYVERKRAELGL"
     gene            4079..5554
                     /locus_tag="PK710_11110"
     CDS             4079..5554
                     /locus_tag="PK710_11110"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOT10314.1"
                     /translation="MSYPSNHLVFSVPFLVFSLILSGCATRTPAAQPPAPVSAPGSAS
                     TTMFGQSAPPLATPPVLAGTPDIASLVEKVQPAVVNITTSASVQMPPMIDPFEFFFGP
                     EGFGRPEGRTPFSIPFPSGPVERERRSLGSGFVIDADGYVVTNNHVIEDADEVMVRFA
                     DDRSFPARVIGRDGKLDVALLKLDGAGVLNPVVLGDSDALRVGEYVIAVGNPFGLGHT
                     VTMGIVSAKDRTIGAGPYDDFIQTDASINPGNSGGPLFNLRGELVGINTAIHRQGQGI
                     GFAIPINMVKDVVLQLRDKGHVQRGKLGITFQPVNEDIARALGLDRPRGALVSEVERG
                     AAADRAGLRVGDLIVEVNGTQLSRSHDLPRLIASQPPGSSVRLQFLREGKPQTVTITL
                     DALEADDKGVGKAAKGASPSRFGLTMQPHANGVVVTAVSGKVASRLVRGDIIQSVDRV
                     PVTTPEQVFAGLTRAKAEKRPALLQVRRGNTTLFVAVEASE"
     gene            5717..6916
                     /gene="ygeW"
                     /locus_tag="PK710_11115"
     CDS             5717..6916
                     /gene="ygeW"
                     /locus_tag="PK710_11115"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF005538.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HOT10315.1"
                     /translation="MTLTASFEDKLAKLASTPTGLHNKDFLLTWEHSDSEIAALLQVV
                     DLLETMYAQGISTRVFDTGLAVSLFRDQSTRTRFSFASATNMLGLTVQDFDEKKSQIA
                     HGETTRETANMISFMTETIGIRDDMFIHEGHKYMTEFASALEDGFRQGVLAQRPSVVN
                     LQCDLDHPTQSLADLQHLIRTYGGVENLRGKRVAMTWAYSPSYGKPLSVPQGIITLMT
                     RFGMDVVLAHPQGYDVIPETLEVAKRHANASGGRFTKVASMQEAFEGADIVYPKSWAP
                     FHVMEQRTKLLRENRSGDLAALEKECLENNKKFMDWECTWDMMKRTRDGRALYMHCLP
                     ADITDVSCEHGEVSADVFDFYRIPTYREASHKPFVIAAMILLTRFADPARVLRNAIER
                     GLPRRLG"
     gene            complement(6935..7702)
                     /locus_tag="PK710_11120"
     CDS             complement(6935..7702)
                     /locus_tag="PK710_11120"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HOT10316.1"
                     /translation="MKPHSIAIVLAVSTVSSASLSQVNVEVVRGDANRQGISGRVEAN
                     VTGNTGNTEGVIAGGSGRLQYRRRKHLVFFHAAGGYTRLNHSTSVERFLFHARYNYEI
                     APWVWLEAFGQVEHDAFRRLTNRELLGAGWRFRLVETEAIDLFYGTSYMSESENVNLA
                     DSDPHQSLTLAHRWNHYLTFSASLSQRLDTGFTAYVQPRFDQFSDYRILHESFFEVGI
                     SDVVSTRLSTWMRYDSDPPQDVKKADFRITNAFVAKF"
     gene            complement(7708..8403)
                     /locus_tag="PK710_11125"
     CDS             complement(7708..8403)
                     /locus_tag="PK710_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT10317.1"
                     /translation="MRTAKLLRSRILAPGVRELTFDPGPDFEFEAGQWISCRIPATYG
                     EDLARSYSLASAPRADGTFDLAVTRVDEGPGSTYLHSLDLGAEVTISRAQGFFTMDEF
                     ERPAIMIATGTGISPFRAMLQALDAQGAFPYPVCLLLGVRTEKDILYGDEFEALARQR
                     NLDFSVTLSQPSQWSGRRGYVQTHLPELVGRFQGQCDVYICGLSKMVKDVRRILKEEL
                     GLTKHQIHSERYD"
     gene            8598..>9623
                     /locus_tag="PK710_11130"
     CDS             8598..>9623
                     /locus_tag="PK710_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOT10318.1"
                     /translation="MSGHYLGVDIGAETIKVVGIEKRAGRVSVTTRLQQQHNKDPHGT
                     LASLLEHVDLVGSQGIAVTGRLQRMVRAEGVPTKAAMRRGVRLLHPDLEAATLLSIGA
                     HGFSVLEMGAHGEEWFQQNSRCSQGTGNFLSQLVKRFGLTVEEASALCNDIVDPAPLS
                     GRCPVILKTDMTHLANKGESRERILAGLYDAVCENVVTLVRPRLAPRDVILIGGVTRS
                     ARVRRMIERWLDGKGMRMIAFRPEHEVLEALGAASHAMEHPGDVASLSELLVTAEATK
                     LERVPALRDSLSRVRRVPRIEMTSWDQPRWVYVGLDIGSTGSKAVAIDETTGEPLWES
                     YLNTEGAP"
BASE COUNT         1994 a         2468 c         3050 g         2111 t
ORIGIN      
        1 tgggggcatt gagatgggaa ttgttggctc cggggctcct ggtcaacgcg gggcttgatc
       61 caggggaaaa tagcttattc gtcaaagcgg gagtcgacgc ccatctttcg gtgctcgacc
      121 ggatgagtga ggtagaagaa gcgcttgccc ggggcggggc ggatgccaag ggcgtcgatg
      181 tggcggtgat tcccatgccg gaatttatcg cggcgtatga gcgattgcgg gcgctcgagc
      241 cccgcgtgtt tttcgtggtg ggctggtcac gcggacgcga agccctgtat gccaagcaag
      301 gttcgctgac caacctaccg gaaggtgagc tgacgttgcg ggccgaacct ggttcctctc
      361 aggcattttt aggcctgttt tcccttcaac tggcgggcgc cccgcccact cgcctccgat
      421 tcgagcttcc cagcgataaa acaccgccgg acttcgaagc cctgagcctg atggacattc
      481 ccaaagtcga cgcggcctcc ggcaaattgc agcttaccag cgccgatgcc ccgatgatgg
      541 tgccgatcgt cgccgtggcg caggcgggtt ttgtggatac ccaccgggcg gcgcttaccg
      601 cttgggtgcg ggtatggctc gagggcgagg accgcattcg agcggatgcg gcaacttctg
      661 ccagggagat atccaagcaa gggggagcgc ccgaccctct ggtgttgatc aaccgtttgg
      721 ggccgttgtc ttgggccaac cttgcggata atgcgcgctg ggccggacta tccggacggg
      781 gagcactgac caccgaatcg ctttttcagc ggggatggga ggtctggaaa gccgctggct
      841 tgctttcgac accgctacct gaggcgccgg catcatcacc gacgatcatt tccgcgctcg
      901 tacggcaatt gggaaccgat caagggacag aaaaattcaa taaaaacagt gacttaccca
      961 ggggcagcca caatgatgtt ctgctgcgct ttgcgttttc tgcggcggag gtcgacgaag
     1021 acgcgttagc gggagaaatt ggatttttgg ctgggttatt cgacaggtcg acgctgcgag
     1081 tggcggttcg aggcacggcg gccatacaca aagcgaaagc ggcaaaggct gtggccgcgg
     1141 cgatcggtcg ttttggtttg gcggaaaatc ggctgcaaat cgcatcgcag cccgcgcgca
     1201 cggcatccgc cacgatcgaa gtgcttgggg cccggtgagc gaaggactca gctcgtcgtt
     1261 ttcggggagc caggcactgg ccaagttctt ataacccatt gatttaacat attaaatata
     1321 cagcatcccc tcgatgggac cctccgcccg ggccgaggcc gatgaggtgg ataagggcgt
     1381 ggcggcgatg agcttatgac gattttggtg ggcgaagggc gattggacat ggtttgggag
     1441 ggctatctct tgcccccggg cgttcgtcgg gctacgcaag ggggcatctg gtgctgccca
     1501 tgaatgaaaa cccatcccaa cgcggtcttt cttccctgct tcgtgtcgtc aagccttacg
     1561 cgaaggacct cattttccgc tcggtagtgc ccaacgagga caatcggtgg cttttcgagt
     1621 tggcgttcga cacccattgg tcggcccggg aagcctatga gtgggcgcgt cgctcgctca
     1681 ttgccacgcc attatttctg tcgaagtgtg cggtgcatgg cgagaggata tcggtagacc
     1741 gcattccgta tattacggcc tgtccgcgca tcgagttggg cagtgatatt cgcatttcgg
     1801 gggagatagc gatcaagggc aactccaggc gttctccggt gctcaagatc ggcaacggag
     1861 tatttttggg tcattggacg acgtttgcgg tggcggagcg gattgaggtg ggggattttg
     1921 tggccatcgg gggcaatacc tacatcgcgg ataccgaggg gcattcccat tacaatccgc
     1981 ggcaaccgat ctggacggtg cctgcatcgg atgaggatat cgagccggtg attatcgagg
     2041 ataacgtaca aatttcgcgg gattgcatga ttctcaaagg cgtgcggatt ggagcgcgct
     2101 cggtcattgg cgcgggcagc gtggtgcgtt ccaacattcc gcccgattcg gtggtgatgg
     2161 gtaatccggc gcgtgtcatc aagcgcatga cgccaccgga ggcggaaaag gctgattctt
     2221 cctcctgaga attccatttc ctcgccaatc tccgagtcaa cggctccttg ctgccatgcc
     2281 gggcgtgatg ggcgggagga ttcgggagca tacggttttg taggggtagc ggcgacgatg
     2341 gtggcgagaa gaaaacaaat gggggaaagc ggcgatagcc gagggcgtct cgggcttgac
     2401 gcaaagccgg ggtgggctca agggtgtggg gtcatgaatc tcgataccat cgatatcaaa
     2461 gcggttgcgc gggacttcgg gacgcctttt tacctatacg atctcgatag cgcccttgcg
     2521 catctgcatg ctttgcaagc tgggctgccg gattatgtcg atgtgtattt cgcgatgaag
     2581 gcgaattcga atccagatgt tcttcgagcg tggcttgggc acgtggcggg tttggatgta
     2641 tcttccgcgg gagagatcga aatggcggta gcggctgggc acagtccttc tgccatgagt
     2701 tttgcggggc cgggaaaaac ggagcaggaa ctacgggttg cgattgagcg gcaggtgcga
     2761 ttgctcagca tcgaatcccc cacggaactt cgccgtgcct cgaaagtggc caccgagctt
     2821 ggcaagacgg caacgatagc gattcgaatc aatccccaat tcactcccaa agaattcacc
     2881 atgaagatgg gaggaacacc gtcgcaattc ggtacggccg aggaagacgc tcccgcgtta
     2941 ttggaagagg ccaaaaccac caaaggcgtt cgtctggacg gcatccacat tttttccggc
     3001 acccaatgcc ttgaaatcaa agggattatt gacaatatcc gtcagaccct ggccattgcg
     3061 agtcgtttga gcgaggagca tgaccttcgt ccatcgttcg tgaacctggg gggcgggttt
     3121 ggggtaccgt attttgcagg acaggaagcg atcgacgtgc atgcgctggc tcgggaggtt
     3181 ggcgagctgc ttcggcaggc gcccaccgag catccgcggc ttcgggacac gcgattcatc
     3241 ctcgaattgg gacggtttct catcggcaca tttggcatat acgtagcgcg ggtggtggat
     3301 gtgaaacaga ctcgcggcaa gcgtttcgtg gtgctcgatg gcggcatgca tcattgtttt
     3361 cctgccacgg gttttttcgg gcaactcgtg aaaaagaact atccggtcgt caacctctcg
     3421 cgtgccgacg gggagccgat ggcgcaggag ttggtgggtc cgttatgtac tccgatggat
     3481 tcgatggcgc gtaacatcac gctaccgcgt ttggaaatcg atgatctcgt ggggattctc
     3541 agtgccgggg cgtattgtta cgccgccagc cccttgttgt tcctggggca tgagactccg
     3601 ccggagattg tagtatacga aggcgcgtac aagctatccc gcgcgagaaa ggcgccgacc
     3661 caattggttt gaacccaagg ttgtggagac acaaggccca tgaacaatat tcgcgaacaa
     3721 attcgcacgt ttatccttac caccttcatg gaaggggccg acgccgactc gttacaggac
     3781 gatacttccc tggagcgagc gcatatcgtc gactcggcgg gaatgctcga gatcatacta
     3841 tttattgagg aaaactatgg tttttcggtg gacaacgacg atgccacgcc ggagaacttc
     3901 gacacgattg atgcgatgac gtcgtatgtc gagcgcaagc gggcagagct tgggctgtga
     3961 ccgggggcgg tgatttgcgg ctgtttggct gattgacgca gcggtcgtga atatcgggaa
     4021 cagactgagc ctctcgttcg ttggaagggg ccgaacccgt tcaacgcagg gtggaacaat
     4081 gtcatatcct tcgaatcacc tggttttttc cgtaccgttt ctcgtttttt cgctcatttt
     4141 atctggttgt gcgacacgga cgccagcggc gcaaccaccc gctcccgtat cggctccggg
     4201 gtctgcctcc acgacgatgt ttgggcaatc ggcgccgccg ttggccacgc cgccggtatt
     4261 ggcgggaacg ccggatatcg cttccttggt cgagaaagtg cagccggcgg tggtcaatat
     4321 caccacgagt gcatcggtgc agatgcctcc aatgatcgat ccgttcgaat tcttttttgg
     4381 accggaaggc tttggtcgtc cggaagggcg cacgccgttt tccattcctt ttccatcggg
     4441 accggtcgaa cgggaacggc gttcccttgg ctcgggattc gtcatcgatg cggacggata
     4501 tgtggttacg aacaaccacg tcatcgaaga cgccgatgag gtcatggttc gtttcgcgga
     4561 cgatcggagc tttcccgcga gggtgattgg acgggatggg aagctggacg tagcgttgct
     4621 caagctggat ggggcgggag tgctgaatcc tgtggtgctt ggcgacagcg atgcgttgcg
     4681 agtcggtgaa tatgtgattg cggtgggcaa tccttttgga ttggggcata cggtgacgat
     4741 ggggatcgtg agtgcgaaag atcgcacgat cggagcgggt ccttacgatg atttcattca
     4801 aacggacgct tcgatcaatc ctggcaacag tggtggtccg ctcttcaatt tgcgaggcga
     4861 actcgttggc atcaacacgg cgattcatcg tcaagggcaa ggaattggtt ttgccattcc
     4921 tatcaacatg gtgaaggacg tggtattgca gctccgggac aaggggcatg tgcagcgggg
     4981 caagctggga attacctttc agcctgtgaa tgaagacata gcgcgagcgt tggggctcga
     5041 tcgtcctcgt ggtgcgctgg tatcggaggt ggaacgaggg gccgcggcgg accgggcggg
     5101 ccttcgcgtg ggcgatctca tcgtcgaagt caacggaacc caactatctc gcagccatga
     5161 cttgccgcgt ctcattgcct ctcagccgcc aggttcttcg gttcgcctcc agtttttgcg
     5221 ggaaggaaaa ccgcaaacgg tgaccatcac gctcgacgcg ctggaagcgg acgacaaggg
     5281 agttggcaaa gcggcaaagg gggcttcgcc ttcgagattc ggtttgacga tgcagccgca
     5341 tgcgaacggg gtggtggtga cggcggtatc gggcaaggtg gcgagtcgat tggtgcgggg
     5401 agatatcatt cagtcggtgg acagggtgcc ggtgacgact cctgagcagg tgtttgccgg
     5461 actgacgcgg gcaaaggcgg aaaagcgacc ggccttgctg caagtgcgaa gaggaaatac
     5521 cacgctattt gtcgcggtag aggcgtcgga atgagcccca cggggcgtgg atgccttggc
     5581 ctacctttgg tccggtgttg aaccgggctt ttccgtggcc tggcgtggag caagcaggtg
     5641 cgccaaagga tggttgtcgg aacaggttct ttttcgtaca aggggtgctt ctcattcgtt
     5701 cattgcgagg aagatcatga cgttgaccgc ttcgttcgaa gacaagctgg cgaagcttgc
     5761 cagcaccccc actggccttc ataacaagga ttttttgctg acttgggagc attcggattc
     5821 ggagatcgcc gcgttgctcc aggtggtcga ccttcttgaa accatgtatg cgcaagggat
     5881 ctcgacgaga gtattcgata cgggacttgc ggtttcgttg tttcgggatc agtcgacgcg
     5941 cacgcggttt tcctttgcaa gcgcgaccaa catgcttggt ctcacggtgc aggacttcga
     6001 cgagaaaaaa tcgcagattg cgcatggtga gacgacacgt gagacggcca atatgatctc
     6061 gttcatgacc gagacgattg ggatccgcga tgatatgttc atccacgaag gtcataagta
     6121 catgacggaa ttcgcctcgg cgctcgagga tgggtttcgg caaggggtgc tcgcgcagcg
     6181 cccatcggtg gtgaatctgc agtgcgacct cgatcatccc acgcaatcgt tggcggactt
     6241 gcagcacctg attcgcacct acggaggagt ggaaaacctt cgtggcaagc gagttgcgat
     6301 gacgtgggcg tattcacctt cctacggcaa acccttgagc gtaccgcaag gcatcatcac
     6361 cttgatgacg cgatttggca tggatgtggt gctggcgcat ccgcaagggt atgacgtcat
     6421 tcccgagacc ctcgaggtgg cgaagcgtca tgcgaatgct tctggaggaa gatttacgaa
     6481 ggttgcatcg atgcaagagg ctttcgaggg cgcggatatc gtatatccga aaagctgggc
     6541 gccttttcat gtgatggagc agcgcaccaa gctgcttcgg gagaatcgct ctggggacct
     6601 ggcggcgctc gaaaaagagt gtctcgagaa caacaagaag ttcatggatt gggaatgcac
     6661 gtgggacatg atgaagcgaa cgcgtgatgg gcgtgcgcta tacatgcatt gtttgcccgc
     6721 ggacatcacg gacgtgagtt gcgaacacgg ggaagtatcc gcggatgtat tcgattttta
     6781 tcgcattccc acgtatcggg aagccagtca taagccgttt gtgatcgccg ccatgatcct
     6841 tctgactcgc tttgccgatc ccgcgcgtgt attgcgaaat gccatcgaac gggggctgcc
     6901 tcgtcggctt ggctaacgga ttcatcgacc ccgttcaaaa cttcgccaca aaggcgttgg
     6961 ttatcctgaa atcagctttt tttacgtctt gcggcggatc gctgtcatag cgcatccagg
     7021 tcgaaagccg ggtcgagacg acatcgctga tacccacttc gaaaaacgac tcatgcaaaa
     7081 tccggtagtc cgagaactgg tcgaaccgcg gttgaacgta agcggtgaaa ccggtgtcga
     7141 gtcgttggct gagggaagcg gagaaggtca gataatgatt ccaacgatgg gcgagggtga
     7201 gggactgatg ggggtcggag tccgcgagat tgacgttctc ggactcggac atgtacgaag
     7261 tgccataaaa taggtcgatc gcttcggttt cgaccaaacg aaaccgccat cctgccccta
     7321 gcaattcgcg gttcgtcaat ctgcgaaacg catcgtgctc aacttgtcca aacgcctcca
     7381 accataccca tggggcgatc tcataattat atcgcgcgtg aaacaggaaa cgttcgaccg
     7441 aggtggagtg attgagccgc gtataaccgc cagcggcatg aaaaaacacg aggtgtttgc
     7501 gtcgacggta ttgcaaacga ccgcttccgc cggcaatcac accttccgtg ttgcctgtat
     7561 ttcctgtcac attggcctcg acccgtccgg atatcccctg acgattggcg tcgcctcgca
     7621 cgacttccac attgacctgc gacaacgatg cgctggaaac cgtgctgacg gcgagcacga
     7681 tcgcaatcga atgcggtttc atgacgatca atcgtatcgt tcggaatgga tctgatgctt
     7741 ggtcaatcca agttcctctt tgaggatccg ccgcacgtct ttcaccatct tcgacaggcc
     7801 acagatatac acatcgcact gtccttgaaa gcgtccgacg agttcgggca gatgggtttg
     7861 cacatagccg cgtcgtccgg accattgaga aggttgcgag agggttacgg agaagtcgag
     7921 gttacgttga cgagccaagg cttcgaattc gtcgccgtac aagatatcct tttcggttcg
     7981 gacgccgagc agtaggcaga cgggataagg gaaagcgcct tgggcgtcga gggcttgaag
     8041 catcgcgcga aaggggctga tgccggtgcc ggtggcgatc atgatggcgg gacgttcgaa
     8101 ctcgtccatg gtaaaaaaac cttgtgctcg tgagatggtc acttcggcgc cgaggtccaa
     8161 cgagtgcagg taggtacttc cggggccttc gtcgacgcgg gtgacggcga gatcgaaggt
     8221 tccatcggct cgaggagcgg aagcgagcga gtacgaacga gcgaggtcct ctccgtaagt
     8281 agctggaatt cgacaagaaa tccattgccc ggcttcaaac tcgaaatccg gtccgggatc
     8341 gaacgtaagc tcacgaacac caggcgcgag gattcgcgag cgaaggagct tggcagtacg
     8401 catgcggtgc caccctggat ccgagcgatg gtaaatgcaa ttgcaaggaa cgaatgaacc
     8461 gttcctgatc gggacaaccg cttggtgcat cttcgaatct cacggaacat atctgtttta
     8521 cagaatgcaa aaacggctag gggtagcgga attcctgaca gctatcccgg ctgacttggg
     8581 gtatgcccgt gggtctcatg agtgggcact atctaggcgt cgatattggc gcggaaacca
     8641 tcaaggtcgt cggcatcgaa aaacgcgcgg ggcgtgtttc ggtcacgact cgtcttcaac
     8701 aacaacacaa caaggatccg catggaacct tggcttccct tctcgagcat gtggacctcg
     8761 tgggttccca aggcattgcg gtcacgggtc gattgcagcg aatggtgcgg gcagaaggtg
     8821 taccgaccaa agctgccatg cgtcgtggag ttcggttgct tcatcccgac ctcgaagcgg
     8881 caaccctgct atccattggc gcccatggtt tttccgtgct cgagatgggt gcccacggag
     8941 aagagtggtt tcagcaaaac tccagatgtt cgcaaggcac cggcaacttc ctatcgcagc
     9001 tcgtgaaacg tttcgggctc acggtcgaag aagcctccgc cctgtgcaac gacatcgtgg
     9061 atccagcgcc tctttccgga cgatgccctg tcattctcaa gacggacatg acgcatttgg
     9121 ccaacaaggg cgagagccga gaacgtattt tggccggctt gtacgatgcg gtttgtgaaa
     9181 acgtcgtgac gctggtgcgg cctcgtcttg ctccccgcga cgtcattctc atcggtggcg
     9241 tcacgcgatc ggcgcgtgtt cgacgcatga tcgaacggtg gctcgatggc aagggaatgc
     9301 ggatgatcgc gtttcgtcct gagcatgaag tgctcgaagc tctcggcgcg gcgtctcatg
     9361 ccatggagca tccgggtgat gtggcatcct tgtcggaatt gctggtaacc gcggaagcca
     9421 ccaagctcga acgagtgcca gcgcttcgcg attcgttgag tcgtgtgcgc agagtgcctc
     9481 ggatcgagat gacatcctgg gaccagccgc gttgggttta cgtgggattg gatatcggct
     9541 cgacgggttc gaaggcggtt gcgatcgatg agaccacggg agaaccgctg tgggaaagct
     9601 atctcaatac ggaaggagcg cca
//
LOCUS       DAOSOR010000175         9568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_7111_length_9568_cov_4.827394, whole genome shotgun sequence.
ACCESSION   DAOSOR010000175 DAOSOR010000000
VERSION     DAOSOR010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9568
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7111_length_9568_cov_4.827394"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            317..2476
                     /locus_tag="PK710_11135"
     CDS             317..2476
                     /locus_tag="PK710_11135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT10319.1"
                     /translation="MLGDSVQSKTATPAPSDLTDIADRTTLQRVIEAFEAQTGVPIIV
                     VDTDGIVWTDSSNSQPQTIDPQTSRLRWVHCMQQLAHDPTMPRVQAIDDDRIREAYAP
                     IIVGNRLLGSYICGPVQIIEKEPSLPPASSIPAMTSAQFDRIASLLQAIASMQSSYGQ
                     RLAEQHATYLPCANASTDLHCLLNAMDDLVFIKDDQHRWVLVNDALCRFMRRRREEII
                     GKSDFDFFPEEEATEFWRMDDEVFRTGKPNINEERLTDGSGITHVISTKKTLLEGSDG
                     KRFLIGVIRDFTERHQEEVELTRHREHLEELVAERTMELSSANRMLQLEIDERLRAEA
                     EKREMQAQIVHQQKLEAIGRLAGGIAHDFNNLLTGVFGHISFALRDETMDPHARESLL
                     LVREAATRAADLIKQLLAFSRKQIIEPRIVDLNALIEKLRRLLDRVLGEDVKLITRYD
                     AHLGNVRVDPVQIEQVIVNLAINARDAMPKGGTLRIETMNRRIRDVGLRDDESMPPGN
                     YAVLVVSDTGVGMDEETKARIFEPFFSTKAKGQGTGLGLATVYGIVKQHRGTIFVTSS
                     VGQGSIFQVLLPCIDAPHEPVFDPSAETILGGTETILLVEDEQVVRQATEGMLEQLGY
                     HVIVAQDGPHALQASEQYQGIIHLLMTDVIMPGMNGKELAELLLQARPNLRVLFTSGY
                     TDDVIVHHGVLDNGVSFISKPFDSAELAGRVRKALES"
     gene            2574..3713
                     /locus_tag="PK710_11140"
     CDS             2574..3713
                     /locus_tag="PK710_11140"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT10320.1"
                     /translation="MNRIVIATVLSISVGALALACSATPRPEPLPPEEPTKALEPTST
                     PVAPEPPPLQEQPKEEPQDQSQPAVDAGPPACPEGMKLVEGEYCTEVEQKCLVEWFAP
                     QNKKRVCEQFEPSTQCVGARVPKRFCIDTYAWPNQAGARPEVMNNFYQAQVKCAAVGK
                     RMCTESEWTFACEGPEMKPFPYGYVRDPRKCNGDHQWDGPSMTKVAKRDPTELARLWR
                     GVPSGSQPHCISDFGVHDLPGNNDEVVAGEKPGAQFSSVHTGGPWYSGVRNQCRPKVY
                     THDEGFYYYFLGFRCCSEPDGQPNETRTPKQVRERMKWEVVERLARFSIAEMQQKLEL
                     KKQGQCTCSERDILCKTMCGTLLGPNAVDGDDSTRIFNVSRDKIR"
     gene            3856..4284
                     /locus_tag="PK710_11145"
     CDS             3856..4284
                     /locus_tag="PK710_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10321.1"
                     /translation="MRFSNSRKNDEGGFEPLARGAATPGHRPHAHKNPRKIHQLCAQV
                     RQVIDLCLAERWDHPLLERVSVVSVTPFPDASRLLILVSVEGDDPIDVQRLLATLRDL
                     RPVLRQEISMEINRKRTPEIVFDLIPDPPPPSFPPAPSED"
     gene            4396..4995
                     /locus_tag="PK710_11150"
     CDS             4396..4995
                     /locus_tag="PK710_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOT10322.1"
                     /translation="MSETTCSQCGAPLENEEEICSNCEATVDSVRSPTKAAPPPPRVA
                     TTPPPQAVEPTPAAARIVADIMIRKVATLFEGEPLEEAEKGFKKVRFRHVPVVGADGH
                     FIGLLSLRHLLRVLPSPFDPDAEARRSAILSQHRVGDIMARNVKTVQASTPLETAARL
                     MLDEKQDCLPVIDDENKLIGVVTLSDFVQLALHLLASQP"
     gene            5046..7322
                     /locus_tag="PK710_11155"
     CDS             5046..7322
                     /locus_tag="PK710_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10323.1"
                     /translation="MTEPVAALWIRVGQQLTALPATGWALAVGVLATITASLWWKPTH
                     LSSRWSSARWFVGGVVVCLAVAHAWSLRWIADDAFISFRYARNLVEGHGLVFNPGERV
                     EGYTNFLWTVLLAGAYAIGIPFGLSAIAFGLLSVVAVLWLTTRLSQLLSPYHHRPLIP
                     VAALLLASNYVFATFGTSGMETLFATALVLLSLERAIQKSYFQAGTAGVCAMMAHPDH
                     AIFFFALAVAIGLGRPSRTTIVRFALPFVLFFLPYFLVRWRYYDSLLPNTFYAKSADE
                     SYFTQGQVYVLISAVSAGIIGWLPLALTGLHTQRSRLFGRFLFIALPPYTLYLLKIGG
                     DFMLGRLLVPWLPLFAISADFAARYLFAKQRRLCATIGLTLAATSALPLRILHPAEKF
                     FHIADERTFYPVETIHPLRIDSPYSTWADQLISTFGEGSHGPLTAIGSIGIVAFQTRY
                     PMLDVFGLTDIEIARQPIVQRGRPGHEKLASPSLLLDRGVLLTDRNVFPPEYASWTEV
                     YPGFIPLHSLAYRTDLVTAIRKTTNSSFTDVPSRIERYEPPADDPYRVACDLWFFETY
                     YFAHQSPQARRQFIHRLLKNGKLSPNTAAFFELSDHDEPRGWSRSLTIDFAQVDKRSW
                     LRSGTAFDAFPTDGPILGQTRVSGHSGPLVNSFTSAQGDGALGMLQSPSFRLDGEVIT
                     LRVGGGKDATRLRAVLVVDGDEVMTATGCDSEILGRRVWDIAKFRGKSARLVVEDRSA
                     DGWGHILLDEFALWRSGS"
     gene            7319..9265
                     /locus_tag="PK710_11160"
     CDS             7319..9265
                     /locus_tag="PK710_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10324.1"
                     /translation="MTPTVPDDSPRLRHQAWFRMALVTFVAGALLLVHARSWMPFFAD
                     DGFISLRYAQRLLDGHGLTWNDGERVEGYSNLLWVLGCAGLGSLGIDLVNASRGLGLT
                     SALGALAALSLSFSGHRHRLLAPLVACVILALSGTLAIWSVGGLEAPMVACFLSWGLY
                     SVRRLLSTPQAQIKKISYIFTSLPFALLCVTRPDGAWIALGMALGIAIFAHGNEPHDG
                     SRKRWVHAAGWLLILPLGFVALQLLFRLSYYGAWLPNPAHAKLAFSYERWVSGSRSLL
                     DAAGPSKALLLLLGAALLPLVKDRQVRERILPFTPVFFGWLLYLVAIGGDIFPARRHL
                     VVPLILSAHAIAETISWYRNRSERHRHWAAIVCVGATILFGIDQAKDPENLRASQERW
                     EWDGQVIGTFFHRHFGPSGAMLASDSAGALPYFSKLPTIDMLGINDRYLATHRPSDFG
                     KGPLGHELGDGHYVWQRQPDLVVFGLPYGQRKALYRSGLEMQDLSGFERDYGLLTFEA
                     TGQTTVTSHVWVRRDSERLGVKRSADRVWIPGMYLAMAQAVAREDGEGNLGTVVSLRS
                     PGAARITLDSGTWRATLVSTGQPVEIGLKPKGQGAMIGTPPLNVSLSGAATHTIDVAV
                     RPLGAGMAHIRSMEFVRTARGPGD"
BASE COUNT         1987 a         2903 c         2544 g         2134 t
ORIGIN      
        1 cattcaagcc gcggaccgtg tcgccgaaca gttgtactcc gaaaactgtc cagcttttat
       61 cgattactac tataaaaaat cctgggctcc cgcacagggc ggagttctca cacaactacg
      121 aaaatcccgg aatcaataac cctccgacaa cgccttccgc cgctttcgtc cttccccgcg
      181 ttgcctagaa aactgcgctt ccttcctgct cgatgatggt acactccagc ccattcgtgg
      241 gccgcagcat ccgatatcca acggtgatgc ctcccaccac actcgtctca gccacgattc
      301 ggaaggggga tttttggtgc ttggtgactc agttcaatcg aaaacggcaa ctcccgctcc
      361 ttcggacctc acggatattg ccgaccgcac gaccctgcaa cgagtcatag aagcgttcga
      421 agcccaaacc ggagtcccta tcatcgtcgt ggacaccgac ggcatcgttt ggacggattc
      481 ctccaacagc caaccccaga ccatcgaccc gcaaacctcc cggttgcgat gggtccactg
      541 catgcaacaa ctcgcgcacg atcccacgat gcctcgggta caagccatcg acgacgatcg
      601 tattcgcgaa gcctacgccc ccatcatcgt gggcaatcgt ctgctcggaa gctacatctg
      661 cggtcccgtt caaatcatcg aaaaggagcc atcgctacct cctgcttcct ccattccagc
      721 catgacatcg gcccagttcg atcggatcgc ctcgctcctt caagcgatcg cctcgatgca
      781 atcctcttac ggccaacgac tggccgaaca acatgccacg tacttgcctt gcgcaaatgc
      841 cagcacggac ttgcattgct tgctcaacgc catggatgac ctggttttta tcaaggacga
      901 ccagcatcga tgggtccttg tgaacgatgc gctttgtcga ttcatgcgtc gacggcgcga
      961 agagatcatc ggtaaatccg acttcgattt ttttcccgag gaggaagcta ccgagttctg
     1021 gcgaatggac gacgaagttt ttcgcacggg gaagcccaat atcaacgagg agcgcttgac
     1081 ggacggctca gggatcaccc acgtcatatc gacaaaaaag acgttgctcg agggcagcga
     1141 cggcaagcga tttctgattg gcgtcatccg tgacttcacc gaacgtcatc aagaagaggt
     1201 cgagctgact cgacatcgtg agcatctcga agaactcgtg gccgagcgga ccatggaact
     1261 gtcgagtgcc aatcgtatgt tgcaactcga aatcgacgaa cgcttgaggg ccgaagccga
     1321 aaagcgagaa atgcaagcac agatcgtcca tcaacaaaaa ctcgaggcca tcggccggct
     1381 tgcgggagga atcgcccatg atttcaataa cttactaacc ggtgtcttcg gccatatctc
     1441 gtttgccctg cgcgacgaaa cgatggatcc gcatgccagg gagtcgttat tgctcgtgcg
     1501 agaagcggcg acccgagccg cggatcttat caagcaattg ctcgccttca gtcgaaagca
     1561 aatcatcgaa ccccgtatcg tcgacttgaa tgccctcatc gaaaagcttc gacgactttt
     1621 ggatcgagtc ctcggggagg acgtcaagct catcactcgc tacgatgcgc atctgggcaa
     1681 cgttcgtgtc gaccctgttc agatcgagca agtcatcgtc aatctggcca tcaacgcccg
     1741 ggatgccatg cccaagggcg gcacgcttcg catcgaaacg atgaatcgtc gaatccgaga
     1801 cgttggcctg cgcgatgacg aatcgatgcc tccaggaaat tacgcggtcc tggtggtgag
     1861 tgacacgggt gtgggtatgg acgaagaaac gaaagcacgt attttcgaac cgtttttctc
     1921 caccaaggcc aaaggacagg ggacgggact cggtcttgcc accgtctacg gtatcgtcaa
     1981 acaacaccga gggacgattt tcgtgaccag ttctgtcggt caaggctcca tcttccaggt
     2041 ccttctgccg tgtatcgacg cccctcacga acccgttttc gatccgtctg cagaaaccat
     2101 cctcggagga accgaaacca tcttgctcgt cgaagacgag caagtcgtgc gacaagccac
     2161 cgaaggcatg ctcgagcagc tcggatatca tgtcatcgtt gcgcaagacg gtccccatgc
     2221 tttgcaagcc agcgagcaat accagggaat cattcatctt ctgatgaccg atgtgatcat
     2281 gcccggtatg aatggtaagg agcttgccga attattgctt caggcccgtc cgaacctacg
     2341 tgtcctattc acctctggtt acaccgatga tgtcatcgtc catcacggcg tgctcgacaa
     2401 cggggtcagt ttcataagca aaccatttga tagcgcggag cttgccgggc gtgtacgcaa
     2461 ggccctcgaa tcgtgatccg taccatctca tcgtgaaccg gcgttcggct ttgacctttc
     2521 ggtcaagctg ggccatgacc ggcctcagcc acgagcccat ggaaagagga atcatgaatc
     2581 gcatcgttat cgcaacggtc ttatcgatat ccgttggtgc attggccttg gcctgctcgg
     2641 cgactccccg tcccgaacca cttccccccg aggaacccac caaagcgctg gagcctacga
     2701 gcacacccgt ggctcccgag ccaccgcccc tccaagaaca accaaaagag gagccgcaag
     2761 atcaatcgca acctgctgtc gacgcgggac cgcctgcctg tcccgagggc atgaagctcg
     2821 tggaaggcga atactgcacc gaagtcgaac aaaaatgtct cgtggaatgg ttcgcgcctc
     2881 aaaacaaaaa gcgtgtttgc gaacagttcg agccttccac ccaatgcgtc ggcgcgcgtg
     2941 tccccaagcg gttttgcatc gatacgtacg cttggcccaa ccaggccggc gcgcgaccgg
     3001 aagtgatgaa caacttctac caagctcagg tcaagtgcgc cgcagtgggg aagcgtatgt
     3061 gtacggaaag cgaatggacc ttcgcctgtg aaggaccgga gatgaaaccc tttccctacg
     3121 gctacgttcg ggatccgcga aaatgcaacg gcgatcacca gtgggatggt ccaagcatga
     3181 ccaaggtcgc caaacgtgac ccaaccgaat tggctcgtct atggcgcggc gttccgagcg
     3241 gttcccagcc ccattgcatc agtgacttcg gtgttcacga tcttcccgga aacaacgatg
     3301 aagtggtcgc cggggaaaaa ccaggcgcgc aattctccag cgtgcatacg ggaggcccct
     3361 ggtacagcgg cgtccgcaac caatgccgac ccaaggtcta cacccacgac gaagggtttt
     3421 attattattt cctcggcttt cggtgctgct ccgagcccga cggtcaacct aacgaaacac
     3481 gcacacccaa acaagtgcgc gaacgcatga aatgggaagt cgtggaacgg ctggctcgtt
     3541 tctcgatcgc agagatgcag caaaaactag agttgaaaaa gcagggccaa tgcacatgct
     3601 ccgaacgcga catcctttgc aaaaccatgt gcggtaccct gctcggccct aacgctgtcg
     3661 atggcgatga ttccacgcgc atcttcaatg tgagccgaga caagattcgg tgaacgttgc
     3721 cggattgctt cgagatgtcg cgctgtccta cccggaccaa agcgcgggtt gaatcaatcg
     3781 ctcgaccccc gtggcccgat catactcgtc tcgcatggtt gccgttttcg tcattttgct
     3841 tcacggtgat cacccatgcg attcagcaat tcgcgaaaaa acgacgaagg ggggttcgag
     3901 ccattggcac gtggcgcggc aacaccgggc catcgccccc atgcacataa aaaccctaga
     3961 aaaattcatc agttatgcgc tcaagtccgg caagtgatcg atctatgtct cgccgaacga
     4021 tgggatcatc cccttctcga gcgcgtgtcc gtggtctctg tcaccccttt tcctgacgcc
     4081 tcccgcttat tgattctggt atccgtcgaa ggcgacgatc ccatcgatgt gcagcgattg
     4141 ctcgccacct tgcgggatct gcgcccggtc cttcggcagg aaatatccat ggaaatcaac
     4201 aggaaacgca ctcctgagat cgtctttgac ctcatccccg acccaccgcc tccctctttt
     4261 cctccggctc cctccgaaga ttgatccatt gtatcgattc ctaccgacat ttccctcttc
     4321 ctttctgttc acgataggcc gttgacgctt cggtcctttg gcttcactat cccctagtac
     4381 cgggaggcat cattcatgag cgagaccacg tgttcccaat gcggcgcgcc cctcgaaaac
     4441 gaagaagaaa tctgtagcaa ttgtgaagct accgtggatt cggtacgttc cccgaccaaa
     4501 gctgccccgc cccctcctcg cgtcgcaacg acaccaccgc cccaagctgt cgaaccaacg
     4561 ccagccgccg ctcgtatcgt cgccgacatc atgatccgca aagtcgccac ccttttcgaa
     4621 ggcgaacccc tcgaagaagc ggaaaagggc ttcaaaaaag tcaggttccg gcatgtcccc
     4681 gtcgtgggag ccgacggtca tttcattgga ttgttgagtc tgcgccattt gctgcgcgtg
     4741 ctgcccagcc ctttcgatcc cgatgcggag gctcgaagaa gcgcaatcct atcccaacac
     4801 cgtgtgggcg acatcatggc gcgcaacgtc aaaaccgttc aagcctccac gcccctcgaa
     4861 acggctgcgc gactcatgct cgatgaaaag caagattgcc tacctgtcat cgacgacgaa
     4921 aacaagttga tcggggtggt gaccttgtcg gacttcgtgc agctcgcatt gcacctgctt
     4981 gcttcgcagc cgtagtcgct gatcattgac ccttccccgc ggcccgacca cgataggcac
     5041 caacgatgac cgagcctgtc gcggcattat ggattcgtgt gggccaacag cttacggcat
     5101 tgcctgccac tggttgggcg cttgccgtcg gcgtgctcgc gaccatcacc gcttctcttt
     5161 ggtggaaacc cacgcacctt tcttcacgat ggtcatcggc acgatggttc gtagggggag
     5221 tcgttgtctg ccttgccgtg gctcacgcct ggagcctgcg ttggattgcc gatgacgcct
     5281 tcatttcctt tcgatacgcg agaaatctcg tcgaaggtca cggactggtc ttcaacccgg
     5341 gagagcgcgt cgaagggtat accaactttc tatggaccgt tttattggct ggagcctacg
     5401 cgatcggaat ccctttcgga ctatccgcca tcgctttcgg cttactgtct gtcgtagcgg
     5461 tactttggct caccacacgg ctttcacaac tcctgagccc ctatcaccat cgtcccctca
     5521 ttcccgtggc ggctcttttg ctcgctagca attacgtgtt cgcgactttc ggaacatcgg
     5581 gaatggagac gctgttcgcg accgcgttgg tgctgctgag cctcgagcgc gcgatccaaa
     5641 aatcttattt tcaagccgga accgccggtg tttgcgccat gatggcccat ccggatcacg
     5701 cgatcttctt cttcgctttg gccgtggcga tcggcctggg ccgcccctct cgcaccacga
     5761 tcgttcgatt cgcactgccg ttcgtgctct tttttctacc ttatttcctt gtgcgctggc
     5821 gttattacga cagtctgctt cccaacacct tctacgccaa atccgcggat gaatcgtatt
     5881 tcacacaagg tcaggtttat gttctgatca gcgccgtatc cgccggtatc atcggatggc
     5941 ttcctttggc cctcacggga ctgcacacgc aacgatccag gttattcggt cgattcctct
     6001 tcatcgctct gcccccgtac acgctgtatc tattgaaaat tggcggcgat ttcatgctcg
     6061 gacgcctcct cgtcccgtgg cttccccttt tcgccatttc cgcggacttc gccgctcgtt
     6121 acctatttgc caaacaacga cgcctttgcg ccacgattgg cttgacgctt gccgccacct
     6181 ccgcgcttcc tcttcgtatc cttcacccag ccgaaaaatt ctttcatatc gctgacgagc
     6241 gaacctttta ccctgtcgaa acgattcatc ctttacgtat cgactcaccg tattccacat
     6301 gggcggacca attgatcagc acgttcggcg agggcagcca cggaccgctg acagccatcg
     6361 gcagcattgg gatcgttgca tttcaaacgc gctatcccat gctcgacgta tttgggctca
     6421 cggatatcga gattgcaaga caaccgattg ttcaaagagg gcgtcccggc cacgaaaaac
     6481 tcgcttctcc atcgctgctt ctcgaccgag gcgtgctatt gaccgaccgc aacgttttcc
     6541 ctcctgagta tgcctcctgg accgaagtct atcctggatt cattcccctg cactcgctcg
     6601 cctatcgaac ggatttggtg actgctattc gaaaaactac aaactcgagc ttcacggatg
     6661 taccttcgcg catcgagcga tatgaacctc ccgcggacga cccgtatcgg gtggcctgcg
     6721 acctgtggtt tttcgaaacc tattacttcg cgcatcaatc accccaagcc cggcgtcaat
     6781 tcattcatcg gcttttgaaa aacggcaaac tctctccaaa taccgctgca tttttcgaac
     6841 tttccgatca tgacgagcca cggggatggt cgcgaagcct gacgatcgat ttcgcgcaag
     6901 tcgataagcg atcgtggctt cgttcgggaa cggcattcga tgcctttccc accgatggtc
     6961 ccatcctcgg acaaacgcgc gtttcgggtc attccggccc tttggtcaac agcttcacca
     7021 gcgcgcaggg cgatggcgcc ctggggatgc tgcaatcacc ttcgtttcgg ctcgatggcg
     7081 aggtgatcac gcttcgtgtg ggaggcggaa aggacgcgac acgattgcgc gcggtgcttg
     7141 tcgtcgacgg cgacgaagtc atgacggcca cgggatgtga ttcggagatc cttggacggc
     7201 gggtctggga tattgcaaaa ttccgcggta aatccgctcg cttggtggtg gaggatcggt
     7261 ctgccgacgg atgggggcat attttgctcg atgagttcgc gttgtggagg tcaggctcgt
     7321 gactccaacg gtgccggacg attcccctcg gctacgacac caagcgtggt ttcgcatggc
     7381 tttggtgacg ttcgtagccg gcgccctgtt gcttgttcac gcccgctcct ggatgccatt
     7441 tttcgccgat gatggcttca tttccctgcg ttatgcccag cgcttgctcg acgggcatgg
     7501 tctgacatgg aacgacggcg aacgggtgga gggctattcc aacttgcttt gggttcttgg
     7561 atgcgcggga ttgggaagct tgggaatcga cctcgtcaac gccagccgag ggctcggatt
     7621 gacaagcgcc ctaggcgccc tcgcggctct ctctctatcc ttttccggac atagacacag
     7681 actcctggca cccctcgtcg cttgcgtgat attggcactg tccggcacgt tggcgatctg
     7741 gtccgtgggc ggattggaag ctccgatggt ggcttgcttc ttgtcctggg ggctgtattc
     7801 ggtccgtcgc cttctttcga ctccccaagc acaaatcaaa aaaatatctt atatattcac
     7861 cagcttacca tttgcgctct tgtgtgtgac ccgtcccgat ggggcttgga tcgcgctcgg
     7921 aatggccctt ggcattgcta tcttcgccca cggaaatgag cctcatgacg ggtcgcggaa
     7981 gcgatgggtt cacgccgcgg gatggctgtt gatcctgccg ctggggttcg tggcgcttca
     8041 gctactattc cggctctcgt attacggcgc ttggctcccc aatccggctc atgccaagct
     8101 cgcattctcc tacgaacgct gggtatcggg cagccgatcg ctgctcgacg ccgcgggccc
     8161 ctcgaaagcg cttctgctgc ttcttggggc cgccctgcta ccgttggtga aggacaggca
     8221 ggtgcgcgag cgcattttgc catttacccc ggtttttttc ggctggctgc tgtatctcgt
     8281 ggcaatcgga ggcgatattt tccccgcccg gcgacatctc gtcgtgccgt tgatcctgag
     8341 cgcccatgcc atcgccgaaa cgatatcctg gtaccgaaac agaagtgaac gccatcgaca
     8401 ctgggccgcg atcgtctgtg tgggagccac tatccttttc gggatagatc aggccaaaga
     8461 tcctgaaaac ctgcgagcga gccaagagcg ttgggaatgg gatgggcagg tcattggcac
     8521 gttctttcat cggcatttcg gcccgtccgg tgccatgctc gcttccgata gcgccggcgc
     8581 tttgccctac ttttccaagc tgcctaccat cgatatgctg gggatcaacg accggtatct
     8641 tgccacccat cgcccttcgg atttcggaaa aggaccgctc ggacacgaat tgggagatgg
     8701 gcactacgta tggcagcgcc aaccggatct ggtggtattt ggcctgccgt atgggcagcg
     8761 caaggcgcta taccgcagtg gtctcgaaat gcaggactta tctggttttg aacgagatta
     8821 tgggctgctg acgttcgaag ccacgggaca gactacggtc acatcccacg tctgggtacg
     8881 acgcgatagc gagcgattgg gagtgaagcg aagtgcggat cgtgtgtgga ttcccgggat
     8941 gtatctggcg atggctcaag ccgtggcgcg agaggatggc gaaggaaatc ttgggacggt
     9001 ggtaagcctt cgatccccgg gagccgcacg aatcaccctg gattccggaa cctggcgagc
     9061 gaccctcgta tcgacgggac agccggttga aataggcctt aagcccaaag ggcaaggagc
     9121 gatgatcggc acgccacccc tgaacgtctc gttgagcgga gctgccaccc ataccatcga
     9181 tgtcgcagtg cgtcctttgg gagccggtat ggcgcatatt cgctcgatgg aatttgtgcg
     9241 aacggcacgg gggcccggcg attgacgtca aaccgggcca tgccatggcc gcaaccgggt
     9301 tgtgcttgac ccaccgtcgg gcacggagtt gtatggcctg ctcaaaaacc aagcgtattg
     9361 aacattttcc cgtgtcggtt cacctccaag gcgaccttcg cttcccgcag gatgccgtga
     9421 gccacgaacc gatccaaagc cgtatcaaga ttgcgggcaa gcccggatcg atgtggcata
     9481 agcagcatag tttaccgcac gcccgcgtac cgtgcggata gccatcatga aggattgcga
     9541 aagccatcac ccatcgtcgc acccttcg
//
LOCUS       DAOSOR010000176         9535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_7141_length_9535_cov_4.555591, whole genome shotgun sequence.
ACCESSION   DAOSOR010000176 DAOSOR010000000
VERSION     DAOSOR010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9535
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7141_length_9535_cov_4.555591"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..730
                     /locus_tag="PK710_11165"
     CDS             245..730
                     /locus_tag="PK710_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10325.1"
                     /translation="MASARGQRGHNFAFTPKGYQESVRGLQRRFAALRLDDEDRPVHR
                     QSTRWCTPPVSARWSLELTEGADVCLRLCDGRVLVGRCLLATAALADDGIRWLSGFVV
                     LRLWGRTVPTRIARTRIVGAMPVGPHSWQERRAIIERQRRGEPAVLVARSGSRNSFFN
                     S"
     gene            complement(760..1038)
                     /locus_tag="PK710_11170"
     CDS             complement(760..1038)
                     /locus_tag="PK710_11170"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT10326.1"
                     /translation="MVRCAAKGAWSTCDAVRPKAVRDVLLELARRIRDARRAAGLTQE
                     AAASRANIDYKRWQRLEEGTVNPTVQTLVRVADALGTDLWHLVGERKR"
     gene            1096..1809
                     /locus_tag="PK710_11175"
     CDS             1096..1809
                     /locus_tag="PK710_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10327.1"
                     /translation="MTDPFKPRDENRRLKILALVCGGLFLVALPILFGDTMCSRQPST
                     VDRMLAEDQQQREQAAREEAARRKREAAEQAEREAADRAAREREQRVAAVAEQYRSAS
                     PAKRMAAVRDECSDRGGCDPLVLTGIAEAAANDGERKALEAYSAKVRKEHALKAGVPE
                     RELFARMYEKALLDQGLNPEKVTATGKYKTTLTVRIWLCSRQFLYDFEKGTIGQQARA
                     AGFTQVECAGGLEGWKQEL"
     gene            1960..2253
                     /locus_tag="PK710_11180"
     CDS             1960..2253
                     /locus_tag="PK710_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10328.1"
                     /translation="MSRQKNPDRSSVTSREGPSVAIRLTDAEYATLQAIVEEQQATAD
                     AAGIVTRVTPSSVLRSLIRAAAQARGLPAFSLQEGASGAAKPKARKTTRKAGA"
     gene            complement(3754..3826)
                     /locus_tag="PK710_11185"
     tRNA            complement(3754..3826)
                     /locus_tag="PK710_11185"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3791..3793),aa:Lys,seq:ttt)
     gene            complement(4009..4926)
                     /locus_tag="PK710_11190"
     CDS             complement(4009..4926)
                     /locus_tag="PK710_11190"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOT10329.1"
                     /translation="MMERKTILITGSEGLIGTALASRLATEGYAIRPLDIRHKSFLFQ
                     GDTTDLPVVKRALRGCCGVIHLAAVSRVLWGQQDPKRCIYTNVGGTKNILRAAIESAT
                     QPWVVLASSREVYGQPEHLPVTEDAPLRPMNIYAQSKVAAERLMGHARNAGIRTSIVR
                     FSNVYGSVRDHPDRVIPAFVRAALQGTALRLDGAEHAFDFTHVDDVVRGVSALVKLVE
                     QGESRLAAVHLVTGISTTLRELAQLVMELGGQTLPLIHAPARSYDVARFQGDPRRAAT
                     LLSWRAQVSLRTGLEKLMADHRASMTHPP"
     gene            5145..8675
                     /gene="recC"
                     /locus_tag="PK710_11195"
     CDS             5145..8675
                     /gene="recC"
                     /locus_tag="PK710_11195"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOT10330.1"
                     /translation="MAKLNVIRSNRLETLVDLMGERLRTQLPANPIAPIDVVVGSRGM
                     ERWLRHRLAMHFGICCNMHFPFPAMKFDALVTNILGESMVEREAWGGASLAFAILDVL
                     PLLVERQEFEAVHGYLASWDAPVSAKAFGLAGQLAAMFERYIDYRPELACAWSRGAQK
                     AAEDDSMHEWQRILWVEVQQRLERPHRAARVAAAIERLKAEQNSHREPLHLFGLTSLP
                     PVWLALIEAYAHHGDVDIYLPCASDSAWQFLRDCIRKDRARWREMSRDTMAEALREEY
                     RRSARPALHPLLLSMGRASRHFQIQLETFVDEENMTDQGRFEGFGRSALRRLQSDVLG
                     ATAPTERETLANTDDSLQFHACYGEIRQVEVLRDVLLGLLEDHRDLEPRDIVVLCPDI
                     ETLGPLITAVFSQGPSARHRKDGEPVQGADGWGPVGAPRIPFEVMDASIRRLNPVADA
                     LLRLLELAEGRFEASAVLDLLSLEPVHRRFSLDDKDLVMLREWVRSSGIRWGRDAADR
                     KRHGQPLDSQNTWRFGLRRLLLGVVLADDGRMVAGREANGEPTNVLPFDDMEGASTAG
                     LGKLVDFLNTLIEVTESLRRARPMTEWVSALEEALDRLTATSKAASWLTLRTRQAIRE
                     LGMDAGGSERLVTREAVTSALSRSMEVLSRATKEQCGAMVFGCLRPMRSVPYRVVCLL
                     GMDDGKFPRKNEMLAFDRTAMAPRAADPSPCDEDRLLLLEALLAAEDHFIVFYTGRDP
                     NTNEPRPACVPIGELRDVIDRTFLPPEGAATPSQWLTKQHPLQSFAESCFLSIHRNPM
                     QPEHPRPWSFDRRLLASATIARGSRVSPPFYPVPSEKKSVPEETIRLEDLISFFKNPT
                     AYLLRRRWKLWLEERPEAVEDREPIELAGLERWQLRQALLDKRLAGTLENEVLERMRA
                     EGRLPLGFAGEALVQGEASSIDALLEKTGIWRKNAKVPQQPAAPLSVDISLPSARLIG
                     TVHPIYGSLLFDFRFGGESTRTILGLWLRLLAWHASEKKAGKAVLALSDGGNTSKSVK
                     LIGYQAPDDCAQVLEPLVQIYRRGCVEPIKLFAGASWSFAWAARDLASKPKVFDKALP
                     DDAELLHKVNAAYEKALEAWFPAYNSPGDVGDPYVSRLFDGLPPMTDRKSKPVPLDLE
                     FARLSMTLWGPLLHHRVTERYVKNWIGDKP"
     gene            8672..>9535
                     /locus_tag="PK710_11200"
     CDS             8672..>9535
                     /locus_tag="PK710_11200"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOT10331.1"
                     /translation="MNTQVHNPVTTWDPMAPLDPGVMLLEASAGTGKTYNLVNWVLRL
                     IVQYHIPIEQQVVVTFTRAATAELKDRIRSRLQQAMTALGNPSSIDPSDIVMERLVAA
                     AQTSREHQEDVVQRIAAAQESFDACLISTIHGFCQRMLQHYAFEAQTDFDLTLMGDEP
                     ELAERLVDDWLSSELYGVDASRYEFLVNQCGFTRDTLLAIATVALREPDMTVVPTDPL
                     LAEGSPAWHRVRFVKWLRSEYDEVHRKRRTQSYIDLMRNLVRVLDKKKSDPSRCSLLQ
                     AVQHRYKVALID"
BASE COUNT         1812 a         2704 c         3135 g         1884 t
ORIGIN      
        1 cggtcccctt gcctttggat tgaggggtcc gttggggggt ggatctgaag cgcgatcgct
       61 gcgcacgcgc ccggcgtcga acgcgacgcc gcggagattg cctcggcggc cttcgccgac
      121 gtggcgcgcc agcttcgtct tgaagctacc gcgagcgacg cgccgtcggt aggtgtcgtg
      181 gttcccgggc gtgcgaccgt gaatcctctt tggtgcgcga tggccctgct tcctcgttga
      241 ccgcatggcg tcggccaggg gacagcgcgg gcacaacttc gccttcacgc cgaagggcta
      301 ccaggagagt gtgcgaggct tgcagcggcg gttcgcggcg ttgcggctgg acgacgaaga
      361 ccggcccgtg catcgccaga gcacgcgctg gtgcacgcct cccgtgtcgg ctcgttggtc
      421 cctggagctg accgaaggcg cggacgtttg cctgcggctc tgcgacggcc gggtgctcgt
      481 gggtcggtgc ctgctcgcca cggccgcgct ggccgacgac ggcatccgat ggctgtccgg
      541 cttcgtggtc ctccggctgt ggggtcgcac ggtaccaacg cgcatcgcgc gcacgcgcat
      601 cgtgggcgcg atgccggtcg ggccgcacag ttggcaggag cggcgggcca tcatcgagcg
      661 gcagcgcagg ggcgaaccgg cggttctcgt ggcacgttcg ggcagtagaa actcgttttt
      721 caactcatag gtgttcacgg tgttcaagcc gacacccgcc taccgctttc gctcacccac
      781 gaggtgccac aggtcagtcc ccagcgcgtc cgcaacccga acgagcgtct ggaccgtcgg
      841 gttcacggtg ccttcctcga gccgctgcca gcgcttgtag tcgatgttgg ctcgtgatgc
      901 ggcggcttct tgcgtgagcc ctgccgcccg gcgggcatcg cggatgcgac gcgccagttc
      961 aagcaggacg tcgcgaaccg ccttggggcg caccgcatcg caggtactcc acgccccttt
     1021 tgctgcacac cgtaccatag tacggtgtgc atcgcgccct gggcgcggtg ccctgatctg
     1081 agggaggcgg cgaagatgac cgatccgttc aagccccgtg atgagaatcg acggttgaag
     1141 atcctcgcgc tggtctgtgg gggcctgttc cttgtggcgc tcccgatcct cttcggagac
     1201 acgatgtgtt cccggcagcc gagcacggtc gatcgaatgc tcgcggagga ccagcagcaa
     1261 cgcgaacaag ccgcgcggga agaagccgcg cgtcgcaagc gcgaagctgc cgaacaggcc
     1321 gaacgggaag ccgccgaccg cgcggcccga gagcgcgagc agcgcgtggc cgccgtggcc
     1381 gagcagtacc gttccgcatc acctgcgaag cgaatggcag ctgtccgcga cgaatgctcg
     1441 gaccgcgggg gctgtgaccc gctcgtgctg accggcatcg ctgaagcagc ggcgaacgac
     1501 ggggagcgca aggcgttgga ggcgtactca gcgaaggttc gcaaggagca cgccttgaag
     1561 gccggggtgc ccgagcgaga gttgttcgcc cgcatgtacg agaaggcctt gctggaccaa
     1621 ggcctgaacc cggaaaaagt gactgcgacg gggaagtaca agacgacgct caccgtgcgc
     1681 atctggcttt gcagcaggca gttcttgtac gacttcgaga agggaacgat cggccagcaa
     1741 gcccgagcgg caggcttcac ccaggtcgaa tgcgcgggcg gcctggaggg gtggaagcag
     1801 gagctttgag ccggttggtt cgcaggtagc gcgtcataga gcagcacgtg aggctcacgc
     1861 acgggcggcg gcaggcccgg tgcggcgcgc agtcgctcac cgacatacac tatgaccatt
     1921 gtgcttgaca caatcatcgg tgctggtatc gttggaacga tgagcaggca gaagaacccc
     1981 gaccgaagca gcgtcacctc gcgggaaggc ccatccgttg ccatccggct caccgatgcg
     2041 gagtacgcta cgctgcaagc gattgttgaa gaacagcaag cgacggcgga cgcggcaggt
     2101 atcgtgaccc gcgtcacgcc atcgagcgtg ctccgctcat tgatccgagc ggcagcacaa
     2161 gcgcgtgggc ttccggcctt ctcgttgcag gaaggtgcat cgggggcggc gaagccgaag
     2221 gctcgcaaga cgaccagaaa ggcgggagca tgaaaccgaa ggcgttgcgg ctagtcgaag
     2281 gcggaaccgc ggcaccaaag ccggcaggcg gttcactccc gcggggtgac ggatggtggg
     2341 tgttcgagcg gcccgaccga agcggccggg cgctggtgct ccgctaccgg gacggcaagc
     2401 gatggcggga ccatcgcctt ccttcggacg tcagaaccga aacggcggcg ctcgaacagt
     2461 acgcgcggag cttcgttgcc gaagtcgcga aggaacggga gcgcatggcg gcggaagcga
     2521 aggaagccgc cggcccgacg ttcaagacct tcgcgcaacg ctggacgtcg ggaaagctcg
     2581 caaaggacca tcccgatcaa gtgcgggaga agcggagcgc cgacgacgac gaagcccggc
     2641 ttgaacgcta cgtctacccg gtgtgcggcg acacgccgat ccgggcgttc acgctggcgg
     2701 acgcggagcg cgtcatgtcg gagctaccgg gcgatttgtc gtccgcttca cggcggcatg
     2761 tggcccaagc tctcaaccgc gttctcaacc ttgcggtgta cccggcgaag ctccgcgacg
     2821 cgaacccgtt gcccaagggg ttcttgccga aggtgacgca ccgaaaggcg gaatcgtggc
     2881 tgtatgcgga cgaggaagcc cggcttgcgg gcgcggagcg cgtgcccctg ttcagccggg
     2941 ttctgtacgg cttccttgca cgggaaggca tgcggatctc ggaagcaacc ggcttggact
     3001 ggtccaacct ggacttgaag cgaggcgcgg tgacgctgga ccgcaacaag accgacgatc
     3061 cgcgcgcgtg ggcgctggac ccgtcggtcg ctagggcgtt gaagcggtac aaggagcgat
     3121 accgcgccga cgcggagccg tccgatccgg tgttctgcga cgcggacggg catcgcatcg
     3181 agaacggcac gacggtcgaa gccgacatat tccgcgagca ccttcggatg gcgggcgtca
     3241 cgcgccccga gctgttcccg gtcgaaggcc agcggcttcg cgcggggtgc cgacggcgca
     3301 aggacttcac gccgggcgag aaagccccgg cggcggacaa cgtacggttg ccgatccggt
     3361 tgcacgacct tcgcgcgacg ttcgtgaccg tctcgctggc gacgggcaag agcgagcgat
     3421 gggtgaagga ccgcaccggg catcgttcga gccagatggt ggaccgctac gcgcgagccg
     3481 cgcgcaccgt tgccgaagtc ggatgctcgg ggttcacccc gatggatctc gcgatcccgg
     3541 agcttcgccc tgacgaagcg gaacgggcat cgcaggagcc cgcttgcgcg acagcgccga
     3601 caccgcaacc ggagcgcgtt gacgcgacgg tgccgaacgc cctgccgcgg acacccctac
     3661 ccgaccgacc tacggattgt catgtggcgc agcatgtggc gcacttcgat gacgcggccc
     3721 ggcaattgat cgaaagtgcc ttgatttata gggtgggcca agagggactc gaaccctcga
     3781 ccgacggatt aaaagtccgc agctctaccg cctgagctat tggccctatc ggaaaaaaat
     3841 ttcgtgtctt ttaccagcag tcatccgctt gtcaatcgtc ttgcgcgaag atcgacgcgc
     3901 ttttctcact gacttcgtat tgtcaacggc gagcggcgtt ctgccacagt tcatgtcatt
     3961 cgtcgagggg ggaaaagatc aaaattatgt ttcctgaaag cgaaggtttc aagggggatg
     4021 tgtcatcgac gcgcgatggt ccgccatgag tttttcgagt ccggttcgta agctcacctg
     4081 ggcgcgccag gaaagcaggg tggccgcccg gcgcggatcg ccttgaaatc gagctacatc
     4141 atacgaacgc gcaggagcgt ggatgagcgg aagcgtctgg ccgcccagct ccatcacgag
     4201 ctgcgcaagc tcgcgcaatg tcgtggaaat gccggtgacc agatgaaccg cggcaagccg
     4261 tgattccccc tgttccacaa gcttgaccag ggctgaaacc cctcgcacca catcatcgac
     4321 atgggtgaag tcgaacgcat gctcggctcc gtcgagacgc aatgccgtgc cttgtagcgc
     4381 cgcgcggaca aaggccggaa tgactcgatc cgggtgatct cgcacgctgc cgtatacgtt
     4441 cgaaaatcgt acgatcgagg tacgaatccc cgcgtttcgc gcgtggccca tcagccgttc
     4501 cgccgcgact ttcgactgtg cgtagatatt catgggacgt agcggagcgt cttcggtaac
     4561 gggcaaatgt tccggttgac cgtagacctc acggctgctt gccaacacga cccatggttg
     4621 cgtggcggat tcgatggccg cgcgaaggat gtttttcgtg ccgcccacat tggtgtaaat
     4681 gcaccgtttc ggatcctgtt gtccccacaa cacgcgggag accgcagcga gatgaatgac
     4741 cccacaacag ccccgaagcg ctcgtttcac gactggcaga tccgttgtat cgccttgaaa
     4801 taaaaaggat ttatgacgaa tatccaaggg ccgaatcgca tagccttccg tcgcgagcct
     4861 cgatgccagt gccgtgccga tcaatccttc cgacccggtg atgagaatcg tttttcgttc
     4921 catcattttc ctacgacgaa agatcgactt ttctgctggc cacgcatacc cgattttggg
     4981 ctgcccgccg ttttctttga ggagatcatg aaagaggcga gcgcatttgt gcttgtctca
     5041 ggggccaggt tctacggtat tctcttcggg attaacacgg tgcttgggga tggctgatca
     5101 cccgcttgcg cgatgggtgt gattgacgtt gggaaggctc gttcatggcg aagttgaacg
     5161 tgattcgaag caatcgattg gagacgttgg tagacctgat gggggagcga cttcgaacac
     5221 agcttcccgc gaatccgatc gcaccgatcg atgtggtggt gggaagccgc ggcatggagc
     5281 gttggcttcg tcaccggctc gccatgcact tcgggatttg ctgcaacatg cactttccgt
     5341 ttcctgccat gaaattcgac gctttggtga cgaatattct cggggaatcc atggtggaaa
     5401 gggaggcctg gggtggggca agtctggcct ttgcgattct cgacgttctt cccttgctcg
     5461 tggaacgcca ggagttcgaa gccgtccacg gctatcttgc ctcctgggat gctccagtca
     5521 gcgccaaagc gttcgggctg gccgggcaat tggctgccat gttcgaacgg tatattgatt
     5581 atcgtcccga gcttgcctgc gcgtggagtc gaggtgcgca aaaagcggca gaggacgact
     5641 cgatgcacga gtggcagagg attctgtggg tcgaagtgca acaacgactg gagcgacctc
     5701 atcgagctgc acgggttgct gccgccatcg agcgcctgaa agccgagcaa aactctcacc
     5761 gggagccgtt gcacctattt ggtctgacga gcctaccacc cgtatggctt gcgttgatcg
     5821 aggcgtatgc gcaccacgga gacgtcgata tctatctgcc ttgtgcatcg gattccgcct
     5881 ggcagtttct tcgcgattgc attcgcaagg atcgagcgcg atggcgtgag atgagccgcg
     5941 atacgatggc cgaagcgtta cgggaagaat accgacgctc ggctcgtccg gccttgcatc
     6001 ctttgttgct ttcgatgggg cgggcgtctc ggcatttcca gatccaactc gagacattcg
     6061 tggatgaaga gaacatgacg gatcaaggtc gattcgaggg cttcggtagg tcggcgttgc
     6121 ggcgtttgca gtcggatgtg cttggggcaa ccgcgcccac ggagcgggag acattggcga
     6181 acacggatga ctccctgcag tttcacgcgt gctatggcga gattcgtcag gtcgaagtgc
     6241 ttcgcgacgt attgcttggg ttgttggagg atcatcgaga tctcgagcca cgcgatatcg
     6301 tggtgttatg cccggatatc gagacactcg gtccgctgat caccgcggta ttttcccaag
     6361 gaccttcggc gcgccatcgg aaagacggcg aaccagtgca aggggcggat ggttggggtc
     6421 cggtcggggc tccgcgcatt cctttcgaag tcatggacgc gagcattcgc aggctcaacc
     6481 ccgtggcaga tgcgctgttg cgtctgctcg aactggcgga aggacggttt gaagcctctg
     6541 ccgtgctcga ccttctctcc cttgaaccgg tgcatcggcg tttttccctg gatgacaagg
     6601 atctggtgat gcttcgagag tgggttcgaa gcagtggtat tcgttggggc cgagacgctg
     6661 ccgatagaaa acggcatggg caacctttgg attcgcaaaa tacgtggcga tttggcttgc
     6721 gccgcttgtt gctcggggtt gtgctcgccg acgatggccg gatggtggca ggccgggagg
     6781 cgaatggcga acccacgaac gtgttgccct tcgatgatat ggaaggggcg tcgacggcgg
     6841 ggcttggaaa gctcgtggac tttctgaaca cgctgattga agttacggaa tcactgcgca
     6901 gagctcgacc gatgacggag tgggtgagcg cgttggaaga ggctttggat cggctcacgg
     6961 cgacttcgaa ggccgcttcg tggctaacgc ttcggacccg gcaggcgatt cgagagttgg
     7021 ggatggatgc gggcggaagc gaaaggctcg tgacgcgcga agcggtgacg agtgcgttgt
     7081 cgaggtcgat ggaagttctg agccgcgcca cgaaagagca atgtggcgcg atggtctttg
     7141 gctgtttacg gcccatgagg agtgttccct atcgagtcgt ctgtttgctc gggatggatg
     7201 acggaaaatt cccacgtaaa aacgagatgt tagctttcga tcgcaccgcg atggccccgc
     7261 gcgcggccga cccatccccc tgcgatgagg accgtctttt gttgttggag gcgctgcttg
     7321 cggccgaaga ccatttcatc gtgttttata cgggccgcga tccgaatacc aacgaacctc
     7381 gacccgcctg tgtgcccatc ggcgaattgc gcgatgtcat cgatcggacg tttcttcccc
     7441 ccgagggagc agcaacaccg agccaatggc tgacgaaaca gcatccactg caatcatttg
     7501 ccgagtcttg ttttctttcg attcaccgaa atccgatgca accggagcat ccacgcccgt
     7561 ggagcttcga tcgtcggttg ttggcgagtg cgaccatcgc gcgcggctcg cgcgtatcgc
     7621 ctccctttta tccggttcct tcagagaaaa agagcgtgcc ggaagaaacg atccgcctcg
     7681 aggacctgat ttcgtttttc aagaatccaa cggcgtattt gttgcgtcgt cggtggaagc
     7741 tctggctcga ggagcggccg gaagcggtgg aggatcgaga gccgatcgag ttggcggggt
     7801 tggaaaggtg gcagttgcgt caagcgttat tggataagcg tttggcgggg acattggaaa
     7861 acgaggttct ggaacgaatg cgggcggaag gcaggcttcc tcttgggttt gcgggggaag
     7921 ctctggtgca aggggaggca agctccatcg acgcgctatt ggaaaaaacg ggtatatggc
     7981 ggaaaaatgc caaggtcccg caacagcccg ccgcgccgct atctgtcgat atctcgctgc
     8041 ccagcgcccg actcatcggc accgtacacc ctatctatgg atccttgctt ttcgattttc
     8101 gctttggggg cgaaagtacc aggactatcc ttgggctttg gctgcgcctg ttggcctggc
     8161 atgcctcgga aaaaaaggcg ggcaaggctg ttttggcgct atcggatggg ggaaatacct
     8221 ccaaaagcgt caaactgatt ggctaccagg cgcctgatga ttgtgcgcaa gttctcgagc
     8281 cattggttca aatctaccga aggggttgtg tggaacccat caagctattc gcgggggcgt
     8341 cgtggagttt cgcctgggct gcccgggacc tggcttcgaa gccgaaagta tttgataagg
     8401 cgctgccgga tgacgccgag ctgctccaca aagtgaatgc cgcgtatgag aaagcgttgg
     8461 aagcgtggtt tccagcctac aattcccccg gagacgtggg tgatccttac gtgagccgtc
     8521 tcttcgatgg cttaccaccg atgacggatc ggaagtcgaa acctgtgcct ctggatctag
     8581 aatttgcacg tctttccatg acgttatggg gtcccctgtt gcaccatcgg gtgacggaga
     8641 gatacgtcaa gaattggatc ggagacaagc catgaatacg caggtccaca atccggtgac
     8701 tacgtgggat ccgatggctc ccctcgatcc aggggtgatg ctgctcgaag cgagcgctgg
     8761 aaccggtaaa acctataacc tcgtcaattg ggtgctgcgg ctcatcgtgc aataccatat
     8821 tccgatcgag cagcaggtcg tggttacgtt cacgcgcgcc gccacggcgg aactcaaaga
     8881 ccgtattcgc agtcgcttgc agcaggcgat gactgcattg gggaaccctt ccagcatcga
     8941 tccgtccgat atcgtgatgg aacgactcgt ggccgctgcc cagacgtcca gggagcatca
     9001 ggaagatgtt gttcagcgta tcgcggcggc gcaggagtcg ttcgatgcct gtttgatttc
     9061 cacgattcat ggcttttgcc agcgcatgct ccaacattat gcctttgaag cgcagacgga
     9121 cttcgatctt acgttgatgg gcgacgagcc ggagctggca gagcggttgg tggatgattg
     9181 gctttcttcg gagctgtatg gcgtggacgc gagccgctac gaattcctcg tgaatcaatg
     9241 tggttttacg cgcgatactc tccttgctat tgccacggta gcgcttcgtg agcccgatat
     9301 gaccgtggtg cctacggacc ctctgctcgc ggagggcagt ccggcatggc atcgtgtgcg
     9361 tttcgtaaag tggttgcgat cggaatatga cgaggtccat cggaagcggc gaacgcagtc
     9421 gtatatcgat ctcatgcgca acctggttcg ggtgctcgac aagaaaaagt cggatccgtc
     9481 tcggtgttct ttgctgcaag ccgtgcagca tcggtacaag gtggctttga tcgat
//
LOCUS       DAOSOR010000177         9448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_7226_length_9448_cov_4.518471, whole genome shotgun sequence.
ACCESSION   DAOSOR010000177 DAOSOR010000000
VERSION     DAOSOR010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9448
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7226_length_9448_cov_4.518471"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PK710_11205"
     CDS             complement(<1..629)
                     /locus_tag="PK710_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOT10332.1"
                     /translation="MSVPHETVSRYSFFESMADAETIKLEYQQGTIAVWNASLEDDDL
                     PSGGKYDPRDGCLRFEGYAYPQLFRWLVRSGRPYEDKARGYQPLSLPRRVHHEPFPYQ
                     REALDAWVKHRRAGVVILPTGAGKTILALLAIELVQRSTLVVVPTLDLVSQWYDVLTR
                     AFGTDVGVVGGGDHDLRELTVTTYDSAQLQAERFGARFGLVVYDECHHLP"
     gene            688..1029
                     /locus_tag="PK710_11210"
     CDS             688..1029
                     /locus_tag="PK710_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10333.1"
                     /translation="MDDRFEEILNRLEAIERRLDEDDYRRGRDMGRWPDRRGEDGRGS
                     CCCHCHDRDHRRDDYPARNRDRGSMMARGFDEKRVVDLIVRLVSERVEQIISHAIRPT
                     PEPESESEQSP"
     gene            1134..1736
                     /locus_tag="PK710_11215"
     CDS             1134..1736
                     /locus_tag="PK710_11215"
                     /inference="COORDINATES: protein motif:HMM:NF016286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HOT10334.1"
                     /translation="MQPKPPDKQQLMNYLLAEAPSVFIHLDPRQEKVVVPPRFRKNPM
                     LILEIGLNMRVPIPDLNVDAEGVTCTLSFGGKPYWCQLPWPSVFALVGSDQRGYVWFE
                     QVPKEVADRYVSPSKSPAPKLKAVDDSEKSEPRTSDKRVVALHASKPSRPPAPDTTMP
                     DTDGNAAGVLEPLEDRPEPSQDDPKKKSKRKLPSYLRVVK"
     gene            complement(1753..2592)
                     /locus_tag="PK710_11220"
     CDS             complement(1753..2592)
                     /locus_tag="PK710_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10335.1"
                     /translation="MGMPRPTMNLSHPERVADLAFLACFRGRKFGRARFARLIQERIG
                     SSEATERFVANVPVELIALFFAREVRPVSDERAPVNECFELPDGGLLEFVGDNMEAAE
                     TIARLLLIHDEPRLDHVVELLIEHDRSWLHTLALEARDARLMELGFPDLDDVDRLYAP
                     WHAQLLIRKLQAWTENSAVQHMRLPRSKITAEALLAYTHDLVEPDRKIALERLHFLAL
                     CQAVHDTRRSIRKMTQDNLNESAATALRILAQGIREAGGDLALLSSPQMATVYRLGLA
                     TLR"
     gene            3178..3972
                     /locus_tag="PK710_11225"
     CDS             3178..3972
                     /locus_tag="PK710_11225"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOT10336.1"
                     /translation="MLRFFKITIYLAVLLIGQADNHVGHSMGWNGGTIGNIFQHQRAG
                     SSNDSVLGKATAPSRDITRLPAQKSKGKQQLAILLAQRHSVRSFSSTPLTLAHLGQLL
                     WAAQGMTHEGRRRTAPSAGALYPLELFVLVGRVEGLAVGVYQYEPQQHALRRVQDGDR
                     RQQVASAAWEQMWLASAPAMIVIAAVYARTAVKYGPRASRYVHIEAGHAAENLFLQAQ
                     DLDLATAVVGAFDDPQVVRILGFSNTIAPLLLMPVGMPHPSPMHPD"
     gene            complement(4353..5252)
                     /locus_tag="PK710_11230"
     CDS             complement(4353..5252)
                     /locus_tag="PK710_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOT10337.1"
                     /translation="MNDFDEKTRVTVVVSNPVEPGEKRINDCLVVIYTKETTLLGKRF
                     VLEQNPTTVGRGADNHIVLDGDSVSRKHCNFLRRGEAWFVVDERSTNGTYVNDDHISG
                     ECRLANGDRIKVGPTIFKFLSGTDVESKYHEEIYRLTIIDGLTQAHNKRYLFESLERE
                     IIRARRHSRPVSVVMFDIDHFKRINDDHGHLAGDVVLKELAQRVQGRIRRDELLARYG
                     GEEFVVMLPETELQGAQSLAETLRLKVMEAPFIFQAEPIRVTISLGCAMLTNEDRTAT
                     DLLKRADERLYDAKRGGRNRVCF"
     gene            complement(5402..7750)
                     /locus_tag="PK710_11235"
     CDS             complement(5402..7750)
                     /locus_tag="PK710_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOT10338.1"
                     /translation="MSPIAMTVILVATLSALAYSLHRRWQLLSVAKAPKDRTDRIPER
                     VWVTIKYAFGQYKMPYYPLAGVAHILIFFGFLVLLLRSLILWGRAYDPSFNFWILGPE
                     PVGGLPLGHLYDMAKDLAAIVVLVGSAVFVYLRVVVKDKRMSLHWEGLLILGIIMTMM
                     VTDILYDGAMLALRHLMPSLGCGMANEAMAATCASVSTVIAPVASVDIPIGFQWFAPA
                     GSVAAMALKGLSAETLVVLAHIGFWTHSTLVLVFANILPYAKHFHIITVMPNVFTANL
                     GPYGRLEPMAKDSEALMELVGNAAELDDPNEVKVGLVRPEHFSWKDVLDFYTCTECGR
                     CSDNCPAYNTGKALSPKQFTIDLRTLFYRNDTMLIDGVGPDEQHEHQDGEHGSEHDEH
                     GEQEHDEHHDKPMPDNPIPNPPIRRQVVNLVPDIIKPEVFWGCTTCRACEEFCPVMIS
                     YVDKMVEFRRNQVLVAGEFPGELAGPFEAMETNGNPWNLSRMDRAAWSEGLDVPTMAD
                     KPSTEVLYWVGCAASYDDRSKKVARAMVRLLKAANVDFAILGEEESCTGDSARRAGNE
                     YLFATLAEQNVAVLNGYQEQGGIKTIVTACPHCFNTLAHEYGDFGGHYQVVHHADFLL
                     RLLREGKLSPKRAVKGKVVFHDSCYLGRYNDIYESPRDALRDIPGLELVEPERYTRNM
                     GVCCGAGGAQMWMEEQNKDRMNVRRTLQLIETNAQTIATACPFCMTMITDGLKDQEKE
                     EQIRQLDIAEILAESCLGNADKESLPAVETSVDSPSSVAVAP"
     gene            complement(7888..8502)
                     /locus_tag="PK710_11240"
     CDS             complement(7888..8502)
                     /locus_tag="PK710_11240"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HOT10339.1"
                     /translation="MHLPFPSIFSHGLRDVMAWASLWLTMSCSSPAKPPSASPIELRR
                     FSGPPDTALDVACSPTGLELCFDAIDNNCNGAIDEGCGVQTGIIQFSIAWSDPTADID
                     LIVMDPSGETARVHKTTRLGLVKDRDCPGMKNECQGQNTENVVLTHHEVPRGVFRVMI
                     RCNALGQTSPPYRVQVGARVGQRSYATVLELEAMGAEKMMTFEL"
     gene            complement(8535..>9448)
                     /locus_tag="PK710_11245"
     CDS             complement(8535..>9448)
                     /locus_tag="PK710_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT10340.1"
                     /translation="TRAYKPLTEYIEDQKENEQARMQACFALGWVTPATKIKEIPEKI
                     TKFATSGDEKDQFIAGCYSETLARYSLPEANGELIKLLKSEMAPMLRLNIAQAIGFTG
                     VRPEDEQPLFELMSNPEVRNNAALALVVGGSEDVASRAVAAYADFPEEALDELRSDYF
                     RVFGYWSDRDFEEGRLYRFVRNAEAIRRVRVRKAPQEWAMFLLGRQFDNLQFDNGPHS
                     MTRVVLRFKLRNAAMTGDPSVRAGALSTLRFMREQGVLMELKQKGGDVGKEAGEALFL
                     LMNPKVVIDVEAPDIKTGGSKILPSNQ"
BASE COUNT         2205 a         2716 c         2507 g         2020 t
ORIGIN      
        1 ggtagatgat ggcattcatc gtagacgaca aggccgaagc gcgcaccgaa gcgttccgct
       61 tgtaactgcg cggaatcata ggtggttacc gtcagctctc gaagatcgtg atcgcctccc
      121 cccacgacac ccacgtcggt tccaaatgcg cgagtcagca cgtcgtacca ctgactgacg
      181 agatcgaggg tgggaaccac gacgagcgtg ctgcgttgaa caagttcgat ggcaagcagg
      241 gcgagaatgg tttttcctgc gcccgtaggc aaaatgacca cgcccgcccg tcgatgcttt
      301 acccaggcat caagagcttc ccgctgataa ggaaatggtt cgtgatggac tcgtcgcggt
      361 aacgatagag gctggtaacc tcgcgccttg tcttcgtagg gacgcccgct gcgcacgagc
      421 caccgaaata gttgagggta ggcgtagcct tcgaaccgta gacaaccatc ccgtgggtca
      481 tactttccac cggatgggag atcgtcgtcc tccagcgacg cattccatac cgcgatcgtt
      541 ccctgctgat actccagctt gattgtttcc gcgtcggcca tcgattcgaa aaacgaatag
      601 cgtgaaaccg tctcgtgggg aacagacacg cgacgatacg ttcaagccgc ccgtgtgata
      661 gagtctcctt ctccaaggag gtccacgatg gacgaccgat tcgaagagat cctgaaccgt
      721 ctcgaagcga ttgaacggcg cctcgacgaa gacgactatc gaagaggacg ggacatggga
      781 cgctggccgg acagacgcgg ggaagatggt agaggctctt gttgctgtca ttgtcatgac
      841 cgggatcatc gtcgcgacga ttatcccgct cggaatcgcg atcgcgggtc gatgatggct
      901 cgaggatttg atgaaaaacg cgtggtggac ttgatcgtgc gtctcgtttc agaacgtgtc
      961 gaacagatca tcagccatgc cattcgcccg acaccggaac cggaatccga gtccgaacag
     1021 agcccctgaa ggatcgaaat tcatgcgggc ttcgggcgtt atgcaggcag catcccgtgc
     1081 aatcgacggg cagggatagt atgtcgtggt tctcattgac ggagtaagtc accgtgcaac
     1141 ccaaacctcc ggataagcag cagctcatga actatctgtt ggcggaagcg ccatcggtgt
     1201 tcattcattt ggatccacga caagaaaagg tggtggttcc cccgcgtttt cgaaaaaatc
     1261 cgatgctcat tctcgagatc ggattgaaca tgcgagtgcc cattcctgac ttgaacgtgg
     1321 atgcggaagg ggtgacgtgt acgttgtcgt ttggaggcaa accgtattgg tgtcagttgc
     1381 cttggccgag tgtgtttgcc ttggtgggca gcgatcagcg aggttacgtt tggttcgagc
     1441 aggttcccaa ggaagtggcg gatcgatatg tgtctccatc gaaatcccct gcacccaagc
     1501 tgaaggccgt ggacgatagc gaaaaaagcg aaccccgaac ctcggacaaa cgagtggttg
     1561 cccttcatgc ctccaaaccg tcgcggccac ccgcgcccga taccaccatg cccgataccg
     1621 atggcaatgc cgctggtgtg ctcgagcctt tggaagatcg gcccgaacca tcacaagacg
     1681 atcccaagaa gaaatcgaaa cgaaaactgc cgtcttattt gcgggtcgta aagtaaagac
     1741 gaggcggtgg tttcatcgga gcgtggcgag acccagccgg tataccgtcg ccatctgagg
     1801 actggacaga agagccaaat ctccacctgc ctcacgaata ccttgcgcaa ggatacgtaa
     1861 cgccgtggcc gcagattcat tgaggttgtc ctgcgtcatc tttcgaatcg aacgtcgcgt
     1921 gtcatgcacg gcctgacata gcgccaggaa gtgcagacgt tcgagagcga tttttcgatc
     1981 gggctcgacg agatcgtgtg tgtaagcgag caaagcctca gcggtgattt tggaacgagg
     2041 caaacgcatg tgttgtaccg cgctgttttc cgtccacgcc tgcaacttgc ggatgagaag
     2101 ctgggcatgc cacggggcat acaatcgatc cacgtcgtcg agatcgggaa atccgagttc
     2161 catcaatcgt gcatcgcgcg cttccaacgc aagggtatgc aaccaacttc ggtcgtgctc
     2221 gatgagaagt tcgacgacat gatcgaggcg aggttcatcg tggatgagca acagtcgcgc
     2281 gatcgtctcg gccgcttcca tgttgtcgcc gacgaactcg agtagaccgc catccggcaa
     2341 ctcgaaacac tcattgacgg gcgcacgttc atcggaaaca ggtcggacct cgcgcgcgaa
     2401 aaacagggca atgagttcga ccggaacgtt cgccacgaag cgctcggtgg cctccgaaga
     2461 gccgattcgt tcttggatga gccgggcaaa tcgggctcga ccgaatttac gtccacggaa
     2521 acaggccaga aaagcaagat ccgccacacg ttcgggatga gaaaggttca tcgtgggccg
     2581 tggcattccc atcgactcac tcttgacctt tcccttctcg ccttttcgct tcgctgcctc
     2641 gggcgacttc gaacgtgccc gcgtctcgtc accgctccga tgaaccatcg gttgcaattg
     2701 tgttcgacgc ttgtcggaag acacacttcg acccgctgtc gatttcgagg atttggcagg
     2761 tttgtctgca cgccgtcttt ggaggcccgc catgcccggt gccctaatga acgtggcgag
     2821 attcgtcaag aaggcaaagg agtcgtccaa taaatagctg taatttcccg actttgccgt
     2881 gctcctccgc gtaccgatgc tagctcggga caaaaaacgc aagatgccag gctaaccacc
     2941 atccaaagaa aatatccgcg atggagccat ccccgaaccc ctatccgcca cccacgaagt
     3001 gtgatgatta gaaaatgaca aaaatcaatg agttgttgaa atagcgggag atgttgcgat
     3061 tttataatat acgatcggat aaatatcgta tgatgacgat tcggtgttgg cgacacgaat
     3121 catgaaatgt catatcgtga atgcgcaatc gatgacaagg ctttgggttc atattaaatg
     3181 ctcagatttt ttaaaattac gatttatctt gcggtcctgc tcattggaca agctgataac
     3241 catgtcggcc actcgatggg atggaacggc ggtacgattg gaaatatttt ccagcatcaa
     3301 cgagccggtt cgagcaacga cagcgtcttg ggtaaggcga cggctccatc gcgggatatc
     3361 acgcgactgc cggcacaaaa aagcaaagga aaacaacagc ttgcaattct actggcgcag
     3421 cgacattcgg tgcgttcttt ctcctccacg ccgcttaccc tggcgcatct cggacaactg
     3481 ctttgggcgg cacaaggcat gacccacgag ggtcgcaggc gtaccgcccc ctccgctggc
     3541 gccctctacc ccctcgaatt attcgtgctg gtggggcgcg tcgaaggact cgccgtcggt
     3601 gtttatcagt acgagcctca gcaacacgcc ttgagacgag ttcaggacgg tgatcgacgc
     3661 caacaggtag cttcggccgc atgggaacag atgtggctcg cctccgcccc cgctatgatc
     3721 gtcatcgccg cggtgtacgc caggacagcc gtcaaatacg gaccccgcgc tagccgatac
     3781 gtgcatatcg aagcagggca tgccgccgaa aacctttttt tgcaagccca agacctcgat
     3841 ctcgctaccg ctgtcgttgg cgctttcgac gatccccaag tggttcggat cctgggtttt
     3901 tccaatacta tcgcacccct attgctgatg ccggtcggaa tgccacaccc ctcacccatg
     3961 catcccgatt gagcgcttca cggcatgacg ataccttgtt atcgaaaaat aacgaattcg
     4021 catgacgaga tttcaagaat tgcgaaagca tatcgcgctc aatagggaat ggcgatggct
     4081 cgaagctgat tcatatttgg cattggtagt atggaatcaa ttggaaagcg cgctgatgac
     4141 tcaccatcga atcgatagga tcgctttcgt agaactcagt acaaacgcga atactggcga
     4201 cctagccatt catgttatta gctggaaaat atcagatccg ccttatgccg tcagcgttat
     4261 cgacatttca tcgccaacga cgatcctagc caagacaacc gagtaccgtc aggcagtgct
     4321 gtgcgcgttt tgtccatcaa ggccaaaaga catcagaagc aaacacgatt gcgaccacca
     4381 cgcttcgcat cgtacaaacg ctcgtcggca cgcttcaaca agtccgtggc ggtacggtcc
     4441 tcgttggtaa gcattgcaca cccgaggcta atggtgaccc ggataggttc cgcttgaaag
     4501 atgaagggcg cctccatcac cttgagacgc aacgtctccg caagagactg ggctccctgc
     4561 aactccgttt ccggtaacat caccacgaat tcctcgccgc catatcgcgc aagcaactcg
     4621 tcgcgacgta tccgcccctg cacccgttgg gccaactcct tgagcaccac atcgcccgca
     4681 agatgaccat ggtcatcgtt gatccgcttg aagtgatcaa tgtcgaacat gacgacactt
     4741 acgggtcgcg aatgccgtcg cgcgcgtatg atctcccgtt ccaacgactc gaagagatac
     4801 cttttgttgt gcgcttgcgt caggccatcg atgatggtca aacgatagat ttcctcgtga
     4861 tacttgctct cgacatccgt gccggaaagg aacttgaaaa tggtaggtcc gactttgatg
     4921 cggtccccat tcgcaagccg acactctccc gaaatatgat cgtcattgac ataggtcccg
     4981 ttggtcgaac gctcgtcgac cacgaaccat gcctcaccgc gtcggaggaa gttgcagtgt
     5041 tttcgcgaaa ccgagtcccc atccaagacg atatggttat ccgctccacg tccgacggtt
     5101 gtggggtttt gttcgagaac gaaacgcttg ccaagtagcg ttgtctcttt ggtgtaaatc
     5161 accacgaggc aatcgttgat gcgtttctcc ccaggctcga cgggattgga gacaacaacg
     5221 gttactcttg ttttttcgtc gaagtcattc acgctacaaa cacaggtcag tgattcgata
     5281 gtcgcaccaa gccgtggcgt attgccccag gccgtgcgga taaacctgta tagaaactaa
     5341 acgagatgat tcttcggcgt caagccaggg aagacggggg aggagcaaaa cagggggcag
     5401 gtcacggggc aacggcaacg gacgaagggc tatcgacgct agtttccaca gcgggcaagc
     5461 tttccttgtc ggcgttgccc agacagcttt ccgccagaat ttccgcaata tcaagctgtc
     5521 taatttgctc ttctttttcc tgatccttga gaccgtcggt gatcatggtc atgcaaaacg
     5581 gacaggcggt ggcaatcgtt tgggcgttgg tttcgatgag ttgaagcgtg cgccgaacgt
     5641 tcatgcggtc cttgttctgc tcttccatcc acatttgcgc tccccccgca ccacagcata
     5701 cgcccatgtt gcgggtataa cgctcaggct ccaccaattc gagacccggg atatcacgca
     5761 gcgcatcgcg cggagactcg taaatgtcgt tgtaacgacc aagataacag gaatcgtgga
     5821 agaccacttt ccccttgacg gcacgcttgg gtgacaattt gccttctcgc aggagacgca
     5881 gcaaaaagtc cgcatggtgg acgacctgat agtgtccgcc gaaatcccca tactcgtggg
     5941 ccagcgtgtt gaaacaatga ggacaggccg tgacgatggt tttgatcccc ccttgctctt
     6001 gatagccatt gagaacggcc acgttctgct ctgccaacgt ggcgaataga tactcgtttc
     6061 ccgctcttcg cgccgagtct ccggtgcatg attcctcttc tccgagaatg gcgaaatcca
     6121 cgttggccgc cttgagcaat cgcaccatcg cacgcgctac cttcttcgat cgatcgtcat
     6181 aactggccgc gcaccccacc caatacaata cctccgtgct tggcttgtcc gccatcgtcg
     6241 gtacatcgag cccctcggac caggctgcac gatccatgcg agataggttc caaggattgc
     6301 cattcgtctc catcgcttcg aaaggcccgg ccaactcacc agggaattcc cccgccacga
     6361 gcacctggtt gcgacgaaac tccaccatct tgtccacgta actaatcatt accggacaga
     6421 attcctcgca agctcggcag gtcgtgcatc cccaaaacac ttccggcttg atgatgtcag
     6481 gaacgagatt gaccacctgc ctgcgaatcg gcggattggg aatgggattg tccggcatgg
     6541 gcttgtcatg atgctcgtca tgctcttgtt cgccgtgctc atcgtgctcg ctgccatgct
     6601 caccgtcttg atgctcatgt tgctcatcgg gacccacacc atcgatgagc atggtgtcgt
     6661 tgcggtaaaa aagcgttcga aggtcaatgg tgaattgctt gggactcaag gcttttccgg
     6721 tgttgtaagc cgggcagttg tcactgcaac gaccgcattc ggtgcaggta taaaagtcca
     6781 aaacgtcttt ccaggagaaa tgctccggcc gcacaaggcc caccttcacc tcgttcggat
     6841 cgtcgagttc cgccgcgtta cccaccagct ccatcaacgc ttccgagtcc ttggccatcg
     6901 gctcgagacg accataaggg cctaggttcg ccgtaaatac gttcggcatg accgtgatga
     6961 tgtggaagtg cttcgcgtaa ggaaggatgt tggcgaacac cagaaccaac gtggaatgag
     7021 tccaaaatcc gatgtgcgca agcaccacga gcgtctcggc cgacaacccc ttcaacgcca
     7081 tggcggccac cgagccggcc ggtgcaaacc attgaaaacc aatgggaata tccacagacg
     7141 ccaccggcgc aatcaccgtc gataccgatg cgcacgtcgc tgccatcgcc tcgttcgcca
     7201 tcccgcaccc cagcgaaggc atgaggtgac gcaacgcaag catcgctccg tcgtacagaa
     7261 tatccgtgac catcatcgtc atgatgatgc ccaaaatcaa aagcccctcc caatgcaagg
     7321 acatgcgctt gtccttcacc acgacccgca agtacacgaa gaccgccgag cccacgagca
     7381 ccacgatcgc cgcgaggtct ttcgccatgt catacagatg ccccaacggc agccccccca
     7441 ccggctcggg cccaagtatc cagaagttga atgacggatc gtacgcccgg ccccaaagga
     7501 tcaacgagcg aagaagcaag accaaaaaac caaaaaagat gagaatatgc gcaacgcccg
     7561 caagcgggta atacggcatt ttatattgac caaacgcata tttgatcgtg acccacaccc
     7621 gctccggaat ccgatcggta cgatccttcg gagctttcgc gaccgacagc aattgccatc
     7681 gacgatgcaa gctgtatgca agggcagaca gcgttgcgac cagaatcacg gtcatcgcaa
     7741 tcggactcat ggcatcctct cccaccgcaa cggggattcg agcacagcac ggttgtgtag
     7801 gaaaaacttg tagaaaagag gacgcggtgc gtcaaacgaa tccaacgact cgagatggga
     7861 accggtcgtt gtcacgaatg cgtttgccta taactcgaac gtcatcatct tctctgcccc
     7921 catcgcctcc aactccaaca ccgtcgcata actgcgctgt ccaaccctcg cccccacctg
     7981 aacgcgatac ggtgggctcg tctgtcccaa agcattgcac cgaatcatca cacgaaaaac
     8041 tcccctgggg acttcatgat gggtgagcac gacattttcg gtgttttgcc cctggcattc
     8101 gtttttcatt cccggacaat cccgatcttt gacaagccct agacgcgtgg ttttgtggac
     8161 ccgtgcggtt tcgcccgacg ggtccatcac gatgaggtcg atgtcggccg tgggatcgga
     8221 ccaggcgatg gaaaattgaa tgatgcccgt ttgcacacca catccttcgt cgatggcccc
     8281 attgcaattg ttgtcgatcg catcgaaaca caattccaat cccgtggggg aacaagccac
     8341 gtccagggca gtatcgggtg gtcccgagaa ccgtcgcaac tcgatggggg aagcagaagg
     8401 aggcttggcg ggcgagctgc aagacatggt gagccaaaga cttgcccatg ccatcacatc
     8461 acgaaggccg tgggaaaaga tgctcggaaa agggagatgc acgcgaagga tgctagcgtg
     8521 atctcttttc gaggtcattg attggatgga aggatcttgg agccgcccgt tttgatgtcg
     8581 ggagcttcga catcgatgac gactttgggg ttcatcaaca gaaagagagc ttcgccagct
     8641 tcttttccga catcgccgcc cttttgcttg agttccatga gaacgccttg ctcgcgcatg
     8701 aatcgaaggg tggacaaagc gcctgcacgc acgctgggat ctcccgtcat cgccgcgttg
     8761 cgcagtttaa accgcaacac cacacgcgtc atcgagtgcg gcccgttgtc gaactgcaag
     8821 ttgtcgaatt gacgaccgag caagaacatg gcccactctt gcggagcctt gcgtacgcga
     8881 acccgtcgaa tggcctccgc gttgcgaacg aatcggtaca accgaccctc ctcgaaatcc
     8941 ctgtcggacc aatatccaaa gacacggaag tagtccgaac gcaattcatc gagagcttct
     9001 tccgggaaat ccgcgtaagc ggctaccgcc cgcgacgcca cgtcttccga accacccacg
     9061 accaacgcaa gagcagcgtt gttgcgcacc tccggattcg acatcaattc gaataacggt
     9121 tgctcatctt cggggcgcac accggtgaag ccaatcgcct gcgcaatgtt cagacgcaac
     9181 atcggagcca tctcggactt gagcaacttg atcagctcgc cattggcttc cggtaatgaa
     9241 taccgagcca gggtctccga ataacaaccc gcgatgaact ggtctttttc gtcgcccgaa
     9301 gtggcaaatt tcgtaatctt ttcaggtatt tccttgatct tggtggcggg cgtcacccag
     9361 cccaaggcaa agcacgcttg catgcgcgct tgttcgtttt ctttttgatc ttcgatgtac
     9421 tccgtcaacg gcttgtacgc ccgggtat
//
LOCUS       DAOSOR010000178         9324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_7332_length_9324_cov_4.333909, whole genome shotgun sequence.
ACCESSION   DAOSOR010000178 DAOSOR010000000
VERSION     DAOSOR010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9324
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7332_length_9324_cov_4.333909"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..1096)
                     /locus_tag="PK710_11250"
     CDS             complement(266..1096)
                     /locus_tag="PK710_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOT10341.1"
                     /translation="MVSRYISFHRCVGLACALGAAALLSACPAVYPEIKTPMRDARAG
                     QVLDPAPPSDVRWIAFKKATIPSLTRDGRRWGNELSSGLPDPYAKLLVNKGPLLETTV
                     QRATLQPTWPDAPAGNFRLRQEDRFRVEIWDARVINDHPIGIRDIGRLDSADISSGVL
                     DLETESGVRVQVAIEPAHARVGYGFFYELRTYDVYVTRVFEESPASRAGIRPGDQILF
                     IGEKLARDMTEGEIRTAFNASRPEGVKLTIKRANGARVDLVLKEGAVYPLFREIGTFR
                     "
     gene            complement(1146..2099)
                     /locus_tag="PK710_11255"
     CDS             complement(1146..2099)
                     /locus_tag="PK710_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10342.1"
                     /translation="MKRVITDNTTLVMLVLGFVVVLLVLLFRNAIVEQTASINPVNSL
                     QAASSTEAQPLQDTNALAPEVTTLSEILQIVTQKARKWRDDARLFRLYATEVKGDGTF
                     DKTRGEIQIILTSEAAEKSGETNGWRGMIRDGNLEATRIWLTPAPALDDRPKRLCSLG
                     EVAGKGAPERFTVDVQWARQQGRQSSLLLFTVEPTRWMVVADPYTCVVGERSSPKTLQ
                     EEQEDKERLVQDESGQEFDARAASESLRKAIERAGCKQGSKTVTGAVQVVFDKRGHAS
                     NVDFLSAMDESEKHCLQAVLMKIRVKPWNRGTGVAATRFAW"
     gene            complement(2151..3995)
                     /locus_tag="PK710_11260"
     CDS             complement(2151..3995)
                     /locus_tag="PK710_11260"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT10343.1"
                     /translation="MSFGVELAAGTIFATDYRVLRPLATGGMGAVYVAEQLSTGKQRA
                     LKLMHAELAWDARLRERFVQEARVGASIDSDHVVEVVGAGVDSVTGMPWLAMELLEGL
                     DLAAYVQQRGPLAFEEVREILSQLCHALAAAHRVGIVHRDLKPQNIFIARSRQQNMTF
                     KVKVLDFGIAKVVAAARHTSAATAVVGTPLWMAPEQTDSQAAVSPASDVWALGLIAFR
                     MLTARWYWMQAAGDSSSVMMLMREVLFEPIVPPSIRAAQMGLAHLIPQGFDAWFQRCV
                     DRNPQARFRDAAEAAQALMAVPGSHAPGSQPLPNSYYLSTNVPSAALPPASLVGASTA
                     VSPYSTASPQDASYFSQLPVQPAPTKRRSFLWIWMVLGIFVVLLAAAGIVLFLGAAAV
                     GIDASANDENNSQNMAGSYVIAQGLNPGGQGRYAGSATIQQLDSTSYRIRWDLTVGAP
                     VEGVGLVSGRVFGVGYGSTPDYTVAVYEIDAGTLRGIYTRPDMRGQVRREVLVGPAGL
                     VGVYQVKQSEQGAGGQVEIRPNGATFFLSRNYGTGVVQGTGIKMNDRLVVTWAKPEGK
                     GGVVAYQIGSGSLEGRWALLTSSLAGTEVLRKNKLGMETVLGTPSSRF"
     gene            4178..4627
                     /locus_tag="PK710_11265"
     CDS             4178..4627
                     /locus_tag="PK710_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOT10344.1"
                     /translation="MPVFRGVYEETFVVDVPLDKAKAHFGNLPMIGKNYDGVDRWEVI
                     DDSTLHVLLKPQEALGSSFRGEHTLKYTFGEQQTTWESEPGGNMRSRGHTRFESLGPT
                     RTRIVHRDEIECDIDINRLLAKALQPIVGLGIERGVKAYLSRMRKAL"
     gene            4636..5304
                     /locus_tag="PK710_11270"
     CDS             4636..5304
                     /locus_tag="PK710_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10345.1"
                     /translation="MPSACTTQQPRAFDRATLELARRRLSRADPKIRTIIRGVGPCTL
                     RTRPLGNHLTALVRSIVFQQLSGKAASTIFHRLLSLVPKRASASDYLRLSDESLRQAG
                     LSRPKIVYLRDLCTKIDNRSLRLDRIGSRTDIEVEQDLVQVRGFGRWSAHMFLLFQLR
                     RPDVWPVDDLGIRKALRLLDGLDSLPSAKTIAYRAEPWRPFRSVAAWYLWRSLETEVP
                     ADFG"
     gene            5404..6330
                     /locus_tag="PK710_11275"
     CDS             5404..6330
                     /locus_tag="PK710_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10346.1"
                     /translation="MSKRSLFGSVVGIAMLLWLAGCGSAQTAASPQSPHADATGYEAA
                     YAPSSAEAQPSYEPAGEAEALPAAPSSMSSRSSSVDGASMIQRPSPQERPGLGTAWGE
                     TRHSPTTSTRFVRDTAAPFAVASLWYNDRQGANAMARSTDYRSYQQGAVPVLSGAITI
                     SLRDDAGRRLDGFYAGGKDYVVGEDGMRYVIVIQNNTRFRYECVASVDGLDVIDGRTA
                     AYTKRGYLLQPHGVLEIDGFRRSADAVAAFRFSSVRSSYASKSGKGDRNVGVIGIAFF
                     NEEGAQPTWSDEEIQRRHDADPFPGRYAEPPQ"
     gene            6652..>9324
                     /locus_tag="PK710_11280"
     CDS             6652..>9324
                     /locus_tag="PK710_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10347.1"
                     /translation="MPSCPLHHGEQLEPKRKGWFCEECGAIVLGYDSIPPPEGTEFVK
                     HGPADTLSPTIKAHSLPCPAADAVTNATVSKGKPERLQRDDVTRWSIVEPAARSSRQT
                     LRALQSTKLSSTIREGHNDGDAVLWACLARPRRGLLVIGPSGVGKTTLLARIVDRALG
                     PETQEDHDDQGVDPRDAVIFLSGRLAYEGEKGIQATRRLCDAVMRCAGIQLGEFDSLH
                     QWMAHLAQSEAKDPKPRRRLWLVFDGIDEANDSDALLEAIDAFLPSLETYGWVRLVVS
                     MADRAYGAVAARGAEYTHGLCGAFVSERWWTWFEEPRSERVRPYLELKGLDETDASVL
                     YHTRQEQMASRSCLVPLCDLAHNIRNLLNIPLYQHLFHEAFRGTKAPPPGLDEVGLVD
                     AFLDALVEQVPGLDHTLSRLGKAMMRRRCASLPGELGRQWNAEWLSQRAERHLKTRTL
                     SLLDPVSELASASGLMVPEGDTTEDREPVAWRFAHERLGEQLLLREMLASIAPRTVPS
                     GEEIVAWAKTAAGSEHEAPFGMLQSALDRWVEQLIAKGETSFVGRLLDIEDRTLRARL
                     IATVLQALQNHESDEDVVSQHARLLAALGHAGMLRNRAPRLLAASWLPLSRLCKTPAS
                     SLGRATHRSIFDVARSAYDGRTSQDDRLAYGSSLLALADAARANEDFEDAHRYLAEAI
                     QLLTDAAAHKGADERMRSVLAAAHWGIAKIEHRQTRLRRARKALDTALKVSSLTDDER
                     QPLPAGQHQERLLSLLLMGDVLSAEGAPQEAAQAYEEALRASLAALAEMPFRSDLRYH
                     HANALIGLAQVAIDSDQPDVVRERFRSALGVLRKLIRREPQRPSYHLALIDTLHRLAK
                     VHRLAAENHEACQLGEEAAAIMLALVQAFPKQQDW"
BASE COUNT         2002 a         2845 c         2489 g         1988 t
ORIGIN      
        1 gtcgcaagag tccccacgcg caccgctcgc ttttcgagat aacacaacac agcgagttga
       61 gaggtggtta gagacgaatc atgcggcata cgaacggaag tgagcattct tcgcaccaac
      121 tctcccatgc gttcgatctg gctctcgata tccacgtcat ggggggcgat acaaagcggt
      181 gaggactcag gtcgcggcat tgtttcgtgg tgcgatataa ttcgcaacct gacgtatgaa
      241 aagcgacaat ttgggaatcg atggctcagc gaaacgtgcc aatctctcga aaaagcggat
      301 agacggcacc ttctttgagc accaaatcca cccgggctcc attcgcacgt ttgatggtca
      361 gtttcacgcc ttccggtcga gacgcgttga acgccgtgcg aatttccccc tccgtcatat
      421 cgcgagctag tttttctcca atgaatagga tttgatcacc gggacgaatc cctgcccgcg
      481 acgctggtga ttcttcgaaa acacgcgtca cgtatacgtc gtacgtcctc aattcataga
      541 agaatccata acctacccgt gcatgggcag gttcaatggc gacctgaacg cgaactccgg
      601 actccgtttc cagatcgagt acacccgacg atatatccgc actatccaac cggccgatgt
      661 ctcgaatgcc gatgggatgg tcattgatga cgcgggcatc ccagatttcc acgcgaaatc
      721 ggtcctcttg gcgaagacgg aagttgcccg cgggagcgtc gggccaggtg ggttgtaacg
      781 tggccctctg cacggtcgtt tcaagaagtg gtcccttgtt gaccaacagc ttggcgtacg
      841 gatcgggcaa tccactcgac agttcgttgc cccatcttcg cccatctcgt gtcagcgagg
      901 ggatcgtggc cttcttgaag gcaatccatc gcacatccga gggaggtgcg ggatcgagaa
      961 cttgaccggc ccgcgcgtca cgcatgggcg tttttatttc cggatatacg gcaggacatg
     1021 ccgaaagaag cgctgcagcc cccaaggcgc acgccaaccc tacgcagcga tggaacgaaa
     1081 tgtatcgcga taccatggcg cgctaggcta gcacgttgga gattcgatgg aagggtgagc
     1141 ctcacctacc aagcaaagcg agtcgccgcc acccctgttc ccctattcca aggcttgact
     1201 cgtatcttca tcaacaccgc ttgcagacag tgtttttccg attcatccat cgcagacaga
     1261 aagtccacgt tcgacgcatg cccacgttta tcgaatacca cctgaaccgc accagtcacg
     1321 gtctttgacc cttgcttgca tccagcccgc tcaatcgctt ttcgcaagct ttcggaagcc
     1381 gcccgggcat cgaactcttg tccggattca tcctgaacca accgctcctt gtcttcttgc
     1441 tcttcttgca acgtttttgg cgatgacctt tcccccacga cacacgtata cgggtccgca
     1501 accaccatcc agcgcgtcgg ttcgaccgta aacaacagca gcgacgattg tcgcccttgt
     1561 tgccgcgccc attggacatc gaccgtaaac cgctccggcg ctcccttccc cgccacctcc
     1621 cccaacgaac acaaccgctt cggtcggtcg tccaacgcag gcgcgggagt caaccaaatc
     1681 ctagtagcct cgaggttgcc gtcgcgaatc atcccacgcc aaccattcgt ttcaccagac
     1741 ttctccgcag cctccgacgt caatatgatc tgaatctctc ctcgcgtttt gtcgaaggtg
     1801 ccatcccctt tgacttccgt ggcatacagc cgaaatagtc gggcatcgtc tcgccatttt
     1861 cgagcctttt gcgtaacgat ctgcaagatt tccgataagg tcgtgacctc cggcgccaac
     1921 gcattggtgt cttgcagggg ctgcgcttcc gtactcgaag ccgcctgcaa cgagttcacg
     1981 gggttgattg acgcggtctg ttccacgatt gcgtttcgaa agaggagcac gagcagcacg
     2041 acgacaaaac cgagtaccag catcaccaac gtcgtattgt cagttattac ccgcttcatg
     2101 aatccatgaa ctcgccacga aagcgtttgt ttcccgacgc tgacaaagcc ttagaaccgt
     2161 gacgatggcg tacccaatac cgtttccatg ccgagcttat ttttgcgtaa gacctcggtg
     2221 cccgccaggg aactcgtcag aagagcccac ctgccttcca gcgatcccga accaatttga
     2281 taagccacca cgccaccttt tccctctggc ttggcccacg tcacaaccaa tcggtcattc
     2341 atcttgatgc cggtgccctg aaccactccc gtgccatagt tacgactcag aaaaaacgtt
     2401 gccccattgg gccgtatctc cacctgccca cctgcacctt gttccgattg tttcacctgg
     2461 tagacaccca cgagccccgc aggcccaacc aacacctccc gtcgtacctg cccgcgcata
     2521 tcgggccgcg tgtagatgcc gcgcaacgtg ccggcatcga tctcgtagac ggcgaccgta
     2581 taatcaggcg tagagccata tcccacaccg aacactcgac cagataccaa gccaacccct
     2641 tccacgggcg ctcccacggt caaatcccat cgaatacgat acgatgtcga atcaagctgt
     2701 tggatcgtcg cagaaccagc gtatcgtcct tgtccaccag ggttgagtcc ttgtgcaatg
     2761 acataggacc ccgccatatt ttgcgaattg ttctcgtcgt tcgcggaggc gtcgatgccc
     2821 accgcagccg cacccagaaa tagtacgatc cctgccgcgg caagcagcac gacaaagatt
     2881 cccaagacca tccatatcca caagaaactt ctgcgtttgg ttggcgcggg ctggacgggt
     2941 agctgcgaaa aatacgacgc atcctgtggt gaagcggttg aataggggga taccgctgtc
     3001 gaagctccca ccaacgacgc tggtggcaat gccgcggaag gcacgttcgt ggaaagataa
     3061 taggaattgg gcaatggctg ggagcccgga gcatggcttc ccggcacagc catcaacgct
     3121 tgcgccgctt ctgccgcatc ccggaagcga gcctgcggat tgcggtccac acatcgctga
     3181 aaccatgcat cgaacccctg tggaatcagg tgggcaaggc ccatttgggc agcccgtatc
     3241 gacggtggaa cgatgggttc gaagagcact tcccgcatca acatcatcac ggaggaagaa
     3301 tcacccgccg cctgcatcca ataccaccgg gccgtaagca tccgaaacgc gatgagccct
     3361 agcgcccaca catcgctggc tggagatacc gcggcttgag aatcagtctg ctccggagcc
     3421 atccataacg gtgtgcccac aaccgccgta gccgcggacg tatgtcgcgc tgccgcgact
     3481 actttcgcga tgccaaaatc cagcactttg actttgaagg tcatgttttg ttgtctggaa
     3541 cgggcgatga agatgttttg cggtttgagg tcccgatgaa cgatgcccac acgatgcgcc
     3601 gcggccagcg catggcataa ttggctaaga atttcgcgca cttcctcgaa cgccagggga
     3661 ccacgctgct gcacgtaagc agcgagatcg agcccctcca aaagctccat cgcgagccaa
     3721 ggcatcccgg tgaccgagtc cacaccagcg ccaacgacct ccaccacatg atcgctgtcg
     3781 atacttgctc ccacgcgagc ttcttgcacg aaccgttcgc gaagacgagc atcccaagcg
     3841 agttcggcgt gcatcagctt cagggctcgc tgctttccgg tggacaattg ctcggccaca
     3901 taaacagccc ccatccctcc tgtcgcaaga ggtcgcagaa cacgataatc tgtagcgaaa
     3961 atcgttccag cggccagttc gaccccaaag ctcatcctcc cgttgtagcg cgttacctcg
     4021 tcccggcaaa gaggctgtca tgtcgattct tctccccctg aatccccgcc tcatgatttt
     4081 caaaggactc cggttttccc atcgtcttca cgtcgttcgg gacatcttct tccgttcgtg
     4141 cgaaccttgc tccctgatgc gcatcgagag gtaaaccatg cctgtgtttc gcggcgttta
     4201 tgaagagact ttcgttgtcg atgttccttt ggacaaagcc aaggcccact tcggcaacct
     4261 gcccatgatc ggaaaaaact acgacggcgt cgaccgatgg gaggtcatcg acgatagcac
     4321 gcttcacgta ttgctcaaac cgcaagaggc cctgggctcc tcgtttcgag gcgaacacac
     4381 cctcaagtac acctttggag agcaacaaac cacctgggag tccgaacccg ggggcaatat
     4441 gcgcagccgg ggccataccc ggttcgagtc gctcggccca acgcgcacac gcatcgttca
     4501 tcgtgacgag atcgagtgcg acattgacat caaccgcttg cttgccaaag ccttgcaacc
     4561 catcgtaggc ctcggcatcg aaaggggcgt caaggcttat ctatcccgca tgcgtaaggc
     4621 cttatgaact cggtcatgcc ttctgcatgc acaacccagc aaccgcgcgc tttcgatcga
     4681 gccaccctcg aactcgcccg tcgacgattg tcacgagccg accccaaaat ccgaacgatc
     4741 attcgtgggg tgggcccctg caccctgcgt acgcgcccgt taggcaatca tttgacagcg
     4801 ctcgtgcgct ccatcgtttt tcaacagctt tcgggcaaag cagcctccac gatttttcac
     4861 cggctgcttt ctctcgttcc caaacgggca agcgcatccg attatttgcg tctcagcgac
     4921 gaaagccttc gtcaagcggg cttgtctcgt ccaaaaatcg tctatctacg tgatctttgt
     4981 acaaaaattg acaatcgttc ccttcgcctc gaccggatcg gctcccgaac ggatatcgag
     5041 gtggaacagg atctcgttca ggttcgcgga ttcggacgat ggtccgcgca catgtttttg
     5101 ctatttcaac ttcgaagacc ggacgtgtgg cctgtcgatg atctgggcat tcgcaaagcc
     5161 ttgcggttgc tcgatggctt ggactcccta ccttccgcca aaaccatcgc ctatcgagcc
     5221 gaaccgtggc gtccattccg cagtgttgct gcgtggtatt tgtggcgatc gctcgaaacg
     5281 gaagtacccg cggatttcgg gtgacccatc ggcgggctgg tggtcggcag aaaaaaaaca
     5341 aagactgtaa gacgcaacga gcaggcgcgt tttcgtgata ttcaacgcaa ataggaggcg
     5401 atgatgagca agcgttcgct cttcggttcg gtggtgggaa ttgccatgct gctttggctt
     5461 gcagggtgcg gctcggctca aacggcagcc tccccccaat cccctcacgc agacgcaaca
     5521 ggttacgaag cagcctatgc tccgagttcg gccgaggcac aaccatcgta cgaacccgcg
     5581 ggcgaggcgg aggccctgcc agccgctccc tcgtccatgt cctcccgaag ctccagcgtg
     5641 gatggcgcct ccatgattca acgcccatcc ccccaggagc gcccgggact cggcaccgct
     5701 tggggagaaa cacgccatag tcccaccaca tcgacccgat tcgtccgaga caccgccgcg
     5761 ccttttgcgg tggcatcgct gtggtacaac gaccggcaag gggccaatgc catggcgcgc
     5821 tcgaccgatt atcgatccta ccagcaaggc gccgttcccg tactcagtgg agccatcacc
     5881 atctcgcttc gtgacgatgc cggccgaaga ctcgatgggt tttatgcagg cggaaaagac
     5941 tatgtggtgg gggaagatgg catgcggtat gtcatcgtca ttcaaaacaa cacgcgcttt
     6001 cgctacgaat gcgtcgcctc cgtggatggt ctggatgtca tcgatggccg gaccgccgcc
     6061 tacaccaaac gaggatacct ccttcagccc catggagtgc tcgagatcga cggttttcgc
     6121 aggagcgcgg atgcggtcgc agcgtttcgc ttcagttcgg tccgaagctc ctacgcatcg
     6181 aaatcgggca aaggagatag aaacgtgggc gtgatcggga ttgccttctt caacgaggag
     6241 ggagcccagc ccacctggtc cgatgaggaa attcaacgtc gtcacgatgc cgacccgttc
     6301 cccggccgtt atgcagaacc tccacagtag tcattatcca cagtatccaa aaacggatag
     6361 tccgaaagcc ccgccttgga ccttcctgcc ctccatggcc gagaagcttt ccccacgact
     6421 gctctaacca tttgattttt cgttattatc acttattttc cccacccatg gggaaagccg
     6481 gatcgcttcg cggtttcgga aagaagcgaa ggattcattc gatttatttc cggaatgagg
     6541 tgccaattcg ctcacctttg tcgacctatc cgggctgttg tcttcgggga acgcaagcct
     6601 gtaggattct tttccgatcc cgtttacagt tggttccgtt acgaggactt catgccatca
     6661 tgcccgcttc atcacggtga gcaactcgaa ccaaaacgaa aaggttggtt ctgcgaagag
     6721 tgcggggcga tcgtgctggg atacgattcc atcccccctc ccgagggaac ggagtttgta
     6781 aaacatggac ctgccgacac cctatcaccg accatcaaag cgcattcctt gccttgtccg
     6841 gccgccgatg ccgtgacgaa tgccaccgta tcgaagggaa aacccgaaag actacaacgt
     6901 gacgacgtca cgcgctggtc gattgtggag cccgcggctc gttcgtctcg tcaaaccctt
     6961 cgcgccctgc aatccacaaa attatcctcg acgattcgtg aggggcataa cgatggggat
     7021 gccgtgcttt gggcatgttt ggctcgaccc cgacgtggtt tgctcgtgat tggaccttct
     7081 ggggtcggta agacaacctt gttggcgcgc atcgttgatc gcgcgttggg accagaaacg
     7141 caagaagacc acgatgacca aggggtcgat cccagagatg ccgtcatctt tctatccggc
     7201 cgcctggcgt atgaagggga aaaggggata caggccacgc gacgtctttg cgatgcggtg
     7261 atgcgatgcg cgggaattca gctcggtgaa ttcgattcgc tccaccagtg gatggcccat
     7321 ctggcgcaat cggaagccaa ggatcccaaa ccaagacgtc ggctgtggct ggttttcgat
     7381 gggatcgatg aagcgaacga cagcgatgcg ctgctggagg ccatcgacgc ctttcttcca
     7441 tctttggaaa catacggctg ggttcgcctg gtggtgtcga tggcagacag ggcttatggc
     7501 gcggtggcgg cccgtggtgc cgaatatacg catggcttgt gcggagcgtt cgtttcggaa
     7561 cgttggtgga catggttcga ggagccccgt tcggaaaggg taaggcccta cctcgagtta
     7621 aaaggattgg atgagacgga tgcgagcgtt ctgtaccaca cgcggcaaga gcagatggct
     7681 tctcgatcgt gtttggttcc gctttgcgat ctcgctcata acatacgaaa tttattgaat
     7741 attcctctat accaacacct gtttcacgaa gcgtttcgtg gcaccaaagc accgcctccg
     7801 gggctcgacg aagtgggatt ggtcgatgcc tttctcgacg cgttggtaga acaagtgccg
     7861 gggctcgatc acaccctgtc cagactcgga aaagccatga tgcgacgtcg atgcgcgagc
     7921 ttgcccggag agttgggacg ccaatggaac gccgaatggc tttcgcaacg tgccgaacgt
     7981 cacttgaaaa cccgcacctt gtcgttgctc gatccggttt ccgaattggc ttccgcttcg
     8041 ggattgatgg ttcctgaggg ggatacgacc gaggatcggg aaccggtggc gtggcgattc
     8101 gcccatgagc gtttgggaga acagctattg ttgcgcgaga tgcttgcttc gatcgcccct
     8161 cggaccgttc cgagtggaga ggagatcgtg gcatgggcca agaccgccgc gggttcggaa
     8221 cacgaagctc cgttcggcat gctgcaaagc gccttggatc gatgggtcga acaactcatc
     8281 gccaaaggcg agacctcctt tgtgggacga ttgttggata tcgaggatcg aacgttacgg
     8341 gcccggctca tcgccaccgt tttacaggct ctacaaaacc acgaaagcga tgaggatgtc
     8401 gtcagccagc atgcccgact cctggcggcg ttgggtcacg cgggaatgct gcgaaaccga
     8461 gcgccgcgtt tgcttgccgc ctcgtggctg ccgttgagcc gtctatgcaa aacccccgcg
     8521 tcgtcgttag gacgagccac ccatcgctcg attttcgatg tggctcgttc cgcctacgac
     8581 ggtcgaacgt ctcaagacga tcggcttgcc tatggttctt ccttgctcgc gctcgccgat
     8641 gcagctcgtg ccaacgaaga cttcgaggat gcccatcgct atcttgccga agccattcag
     8701 ctccttacgg atgcggccgc ccacaaggga gccgacgagc ggatgcgcag tgttttggct
     8761 gcggcccatt ggggaatcgc caaaattgaa caccggcaaa cacgcctccg tcgcgcacga
     8821 aaagccttgg atactgcttt gaaagtatcg agcctcaccg acgacgagcg tcaacctttg
     8881 cccgcggggc agcatcaaga acgccttctt tctcttctgc tgatgggaga cgtattgagt
     8941 gcggaaggcg ctccccaaga agctgcccag gcttatgaag aagcacttcg agcttccctc
     9001 gcggcattgg cagaaatgcc gtttcgctcc gacctgcgtt accaccatgc caatgctctc
     9061 atcggcctgg cacaagtcgc tatcgattcc gatcaacccg atgtcgttcg cgagcgattt
     9121 cgctcagccc tgggcgtatt acgcaaactc attcgccgag aacctcagcg accaagctat
     9181 catctagcgc tcatcgatac cctgcaccgt ctcgccaaag tacatcgcct cgccgccgaa
     9241 aaccatgaag cttgccagct tggcgaagaa gccgcggcca tcatgcttgc gctggtgcaa
     9301 gcgttcccca agcagcagga ttgg
//
LOCUS       DAOSOR010000179         9319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_7342_length_9319_cov_3.791343, whole genome shotgun sequence.
ACCESSION   DAOSOR010000179 DAOSOR010000000
VERSION     DAOSOR010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9319
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7342_length_9319_cov_3.791343"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="PK710_11285"
     CDS             complement(<1..881)
                     /locus_tag="PK710_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10348.1"
                     /translation="MREKRGVLVALALLGIGAAGCWDDEVTTNPVDAGVDVRPDTSLP
                     DAPLDTQTEAATETGTETGPETGPDTGKDVESETTNPDVVEDVVLPPPSGTIQVLTIN
                     DFHGQIDPITVKDKDGYTNYLGGVAYASTYFQKERAANPNTIILNGGDEVGASPTLSA
                     YGTDLRDEPAIVSLNYLGVNASNIGNHSFDDGTAHFKDLMEIADFDYVGVNVEDPKNE
                     LGGHVVKPYKMLTFGDPANPIKVAVVGVSNPETEEIVIAGHMGDVKITEPVAPANAAI
                     AQARADGAHVVLVTAHIG"
     gene            complement(912..3068)
                     /locus_tag="PK710_11290"
     CDS             complement(912..3068)
                     /locus_tag="PK710_11290"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT10349.1"
                     /translation="MRRRTPAIIAGLFLLAVGANCSLIASSELKNGIGASCSSDDDCQ
                     GGVCSDGLCSLECSTSDNCPDPAICISGLCKLGCIEDDACGEGQICEGNACQVGCRND
                     QKCGSGQICQNLTCVTGCRADEPCGAGKICEHNACVDGCRTDTSCGTGKICEQNTCVA
                     GCRTDNQCGEVSDGKICVDKECVTGCRNDDYCASQVGTICNTETNECVSGCKVDDTCG
                     KGFICEKKECVPGCRNNDGCLDGDYCSSEKQCKPTLKVAAVFSGDSTRPAEDALTASH
                     KLGLDQAVASADYVLFGKDRYRITDNASTAQAVEKAIGDAVAAGAKTITTHTPSANAQ
                     ALVAAAKFPNVNFILTGARDRNSLPNVGAYSGKSDQQWYATGRLAARRADKGTKCIGL
                     VLPTATRQIVRETNAFARGVASFDPDIKVVLRWLGATRDRDPSGQPTYTYKAQNYEFD
                     TATDGKLYREELLAAQLADMGCTVIGHRTDTQRVISFIDLIANRVNVAKPHPANYNLL
                     SLGVDMKDQCRTNANASGSWIPTCLGLPYWNWGPLYSKIFDEMNRDAWLGQETRWPFQ
                     VGASAIMKFELSPNTTTTGITTTDVNNVLAAVANDGWDKVFKGPYSFNGQRDLDRDGV
                     ADPDQNLTSTQKLSEEEVDRMCWFVQGVWELPLYKDIVLATVIPAMVPYGPPVSGQVT
                     ELNNTPASKDKYGDVATFITTKLSQNPSEVMSCPLN"
     gene            complement(3071..4042)
                     /locus_tag="PK710_11295"
     CDS             complement(3071..4042)
                     /locus_tag="PK710_11295"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOT10350.1"
                     /translation="MILRTGSASNLLFVLLLFSPLVVSTPAGAQTAPEEQDASEAAAR
                     YRRGLDLYNEENYRGALAEFRKSYEITGEYRVLYNVAQVCYQLQDYVCAVTSFEKYLK
                     DGGNNVSADRRQSVENEISKLRPRIATVSIAVNVEGASVVIDDIPVGKTPLDTILLSA
                     GAHRVAVSKEGYIPISQAIEVAGAEKRTIRLQLEPAGSSRVVVRETERIKEPESQWTT
                     LSYIGLGVGGALLVGGGVTGAMALSSSSKMEKEQYVGTPSDRAKDLQSDVQAYRLTSD
                     ILSAAGILTLGATLYFTLSRDVEPEKTPEPSVGLFVGPGSASFAGSF"
     gene            complement(4061..5728)
                     /locus_tag="PK710_11300"
     CDS             complement(4061..5728)
                     /locus_tag="PK710_11300"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT10351.1"
                     /translation="MARPRRLGKFRFIAEIGHGGMAEVFLAVMEGPARFSKLIALKIL
                     REQFAEQPEGREMFLNEARLAARLSHPNVVSVQEVGEEDGRLFIAMEYLQGQPMSRLM
                     SRIRKSEEELVPLPVLLRILSEALAGLAHAHECTDYDGTPLQLVHRDVTPHNIFVTYD
                     GTVKMLDFGIAKAVSVSTQTRAGTIKGKVSFLAPEQLLGEASLDLRVDIYALGCCLWE
                     ALAKQRPYQGMSDVAVMTRVASVGAPPIREAVPHIHPELERICMKAIAFRPEDRYQDA
                     LEFQSDLDQFILSLGQPIGRKEISAHMMRVFAEDRAKARALIEANLSAASLTSTDEFS
                     VASLATASGSWPDSSSSSRSGMDRNVEFSVPTELPAARKTKMWAVVVGVGGLLLVGAL
                     VIGLNSGDAATDTPPVTASASAEQPVGTIHLQVSAQPSLARMFLDGKTLATNPMELTV
                     PKDGEKHVIRAEAPGYVGKAEQIRFDRDQVIQFELEKHELPADTSAASTTAPPAKVGP
                     VVGPHPRGPKPTAEPAEPTTATPPPVPTPDPSKGKTKRKLDDDNPFE"
     gene            complement(5961..6932)
                     /gene="corA"
                     /locus_tag="PK710_11305"
     CDS             complement(5961..6932)
                     /gene="corA"
                     /locus_tag="PK710_11305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOT10352.1"
                     /translation="MVTRAHLVDSEGRYVIRDFSEVPALIRAKDRTVWIETDERRAEV
                     ESFLQEQMGIHPLTVEDIFFDQPTPKVEDYGDILYIVLHGIHREAQDPESLGTVEIDI
                     LIGPTWVFTHHPRSMRSVEGLRAELERNPRALLRGSAYVAHAIIDRLADDYYPVIEAF
                     DDDIDEVSRQIIEEPVPDILTRLFALRRSLQALRRVSTYQRDVLLRLSRGEFGVIPEH
                     ALPFFRDAYDHFVRIADHADSYRELVNASVEMYMSSAANRTNEIMRVLAIISTIMLPL
                     SFIAGVYGMNFEYMPELRWRWGYLFVWGLMVLVVVGLLTYFRRKRWL"
     gene            7266..8606
                     /locus_tag="PK710_11310"
     CDS             7266..8606
                     /locus_tag="PK710_11310"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HOT10353.1"
                     /translation="MSPSLRLASVCSLSLIFSATCGAGCQSAAGDSKPYVSTPENDAS
                     QDEPDANELDAVEEPEQETSFIDPDTGTKESGVVVVEPPCDNANLYGDEDGDGWSVAD
                     GDCNDCTPLMNPGAYDYPGSKVDEDCNGIVDDEPKGCDEGLPIEGNDPMDAVRALGLC
                     RTADPNAPMAKKSWGVLSARYVFPDGSTASRTPKQFGPHCVGDGQKGTPPNSLSRGIL
                     THFGNVTVPTGGKTMLALSTGVARSGSQGTSPAGAQMCTSSHTPPGFPTPSKAACPNQ
                     KIDEDDTAYDAIALEIEIRTPTNAEGFSFDFNFHTYEYPNFICSQYNDFFVALLWPVH
                     ATNVLHNNISFDPQGNPVSVNNGFLEACLPGTHGGKTFPCPLGIDELAGTGFDSRGAT
                     GWLRTTAPIEPGETIKLRFAIWDMGDDAYDSTVLLDNFTWAVESLPPFTDRPPK"
     gene            complement(8621..>9319)
                     /locus_tag="PK710_11315"
     CDS             complement(8621..>9319)
                     /locus_tag="PK710_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10354.1"
                     /translation="PSRVMEMLEPGSDSQRQALEGSGCETVEGADGNSYVIDAFEDAT
                     GIRMQVALGYAEPEVMGALVAAVLGDLVEEHDDPRGVALYPETYQPRARTWAGLLEEM
                     GDALDWAPLESAEVMLGDFGSELDFQGDLVSLMAQMQEQVSPEMLEKAMRFAQHLIGN
                     GQGTGLSQAEQELLPDMAQLPNFPMDFGTLAEQAQRLFEANPQLEQRLAEPLGMTDAV
                     VDDGDDESGEPRDS"
BASE COUNT         2058 a         2885 c         2351 g         2025 t
ORIGIN      
        1 ccgatatgag cggtcaccaa gacgacatga gcgccatcgg ctcgggcttg ggcaatggca
       61 gcgttggcgg gagcgacggg ctcggtgatc ttgacatctc ccatgtgtcc agcgatgaca
      121 atttcttccg tttcgggatt ggacaccccc acgacggcca ccttgatcgg gttggctgga
      181 tcgccgaaag tgagcatttt gtacggcttg acgacatggc cgccgagttc gtttttggga
      241 tcttcgacgt tgacccccac ataatcgaag tcagcgattt ccatcagatc tttgaagtgc
      301 gccgtgccat cgtcgaagct atggttgccg atgttgctcg cattgacgcc caaataattc
      361 aaagagacga tggcgggttc atcgcgcaaa tctgttccgt aggcgctgag cgtggggctt
      421 gcgccaacct catcacctcc attgagaatg atggtgttgg ggttcgccgc tctttctttc
      481 tggaagtagg tcgaagcgta agcgactccg cccaggtagt tggtatagcc atccttgtct
      541 ttcacggtga tcgggtcaat ctgaccgtgg aagtcattga tggtcagcac ttggatggtg
      601 ccggaaggag gcgggagaac gacatcctcg accacatcgg ggttggtcgt ttccgattct
      661 acgtctttcc ccgtatcggg tcccgtttcc gggccggttt ccgtacccgt ttccgttgct
      721 gcttcggttt gcgtatccag gggcgcatcg ggaagtgacg tatccggtcg cacgtcaacc
      781 ccggcatcca ccgggttggt ggtgacttcg tcatcccaac accctgccgc tccaatcccg
      841 agcagcgcaa gagcaacaag aacacctcgt ttttcgcgca ttgtctttgc ctttcctgag
      901 ctgattggtg gctagttgag cgggcagctc atgacctcac tcgggttttg cgacaacttc
      961 gtcgtgatga acgtcgcgac atctccatat ttatctttgg aagcgggagt attgttcaat
     1021 tccgtgacct gcccggaaac cggaggacca taaggcacca ttgcgggaat caccgtggca
     1081 agaacgatat ccttatagag cggtaactcc catactccct gcacgaacca gcacatacgg
     1141 tccacttcct cctcggagag tttttgggtg ctggtcagat tctggtcggg atccgcgaca
     1201 ccgtccctgt cgagatcccg ctgcccattg aaggaatagg gacctttgaa caccttatcc
     1261 caaccatcgt tggccaccgc ggctagcacg ttattgacgt ccgtagtcgt aatgccggtg
     1321 gtggtggtat tggggctaag ctcgaatttc atgatcgccg aggcccccac ctggaaaggc
     1381 catcgagttt cctggccgag ccaagcatcc cggttcattt cgtcgaaaat cttgctatat
     1441 agcggacccc aattccaata gggcaaaccc aggcacgtgg ggatccagga accggaagcg
     1501 ttggcgttgg ttcgacattg gtctttcatg tcgacgccga gagacagaag gttgtagttg
     1561 gcgggatggg gtttggcgac attcacccga ttggcgatga gatcgatgaa gctaatcacg
     1621 cgctgcgtgt cggtgcgatg cccgatcacc gtgcatccca tatcggctaa ttgcgcggca
     1681 agcagttctt cacggtaaag ctttccatct gtcgccgtgt cgaattcgta gttctgggcc
     1741 ttgtaggtgt aggtaggctg acctgatgga tctcggtccc tggtggcgcc aagccatcga
     1801 aggacgacct tgatatcggg gtcgaaggat gccacaccgc gcgcaaaggc attggtttcg
     1861 cgcacgattt gccttgttgc cgtaggaagg accaacccga tgcatttggt gcctttgtcg
     1921 gcacgtcgcg cggcgagtct acccgtggca taccactgtt ggtcgctctt tccagaatat
     1981 gctcccacgt taggcagtga atttcggtca cgggctcccg tcaaaatgaa gttgacgttg
     2041 gggaacttgg ccgcggcgac caaggcttgc gcgttcgccg acggagtatg ggtggtgata
     2101 gttttggcgc ccgcggcgac cgcatcgcca atggcttttt ccacggcttg cgcggtggaa
     2161 gcgttatcgg tgatacgata gcgatctttg ccgaacagca cataatcggc cgaagccacc
     2221 gcctggtcca atcccagctt gtgactcgcg gtcagggcgt cttccgccgg acgagttgag
     2281 tccccggaaa aaaccgcggc cactttcaat gtcggcttgc actgcttttc actactacag
     2341 tagtccccat ccaaacagcc atcgttgttc cgacacccgg gcacacactc tttcttttcg
     2401 catataaatc cttttccaca cgtatcgtcg accttgcacc ccgacacaca ctcattcgtc
     2461 tccgtgttgc agatcgtccc cacctggctg gcgcaataat catcgtttct gcatccggtg
     2521 acacactcct tgtccacaca gatttttccg tcggagactt caccacattg attgtcggtt
     2581 cggcatccag cgacacaggt attttgttcg cagattttgc cggtaccgca ggatgtatcg
     2641 gttcgacagc cgtcgacgca cgcgttgtgt tcgcagattt tgccagcgcc gcagggctca
     2701 tctgctcggc agccggtaac gcaggtaagg ttttggcaga tttgtccgga tccgcatttt
     2761 tggtcgttcc tgcatcctac ctgacaggcg tttccctcac aaatctgccc ctcaccacag
     2821 gcgtcgtctt cgatacagcc aagcttacac aagccggaaa tacagatagc cggatcggga
     2881 cagttgtccg acgtggagca ttcgagggaa cacaagccat cgctgcaaac gcctccctgg
     2941 cagtcgtcat ccgacgagca agaggctccg ataccgtttt tgagttctga cgaagcgatc
     3001 aaggagcaat tggctcccac cgcgagaaga aaaaggcctg caatgattgc tggagtacga
     3061 cgccgcatgg ttagaagctc ccggcaaagg acgcgctgcc tggtcccacg aaaaggccga
     3121 cgcttggctc aggggttttt tcaggctcca catcacggga caaggtgaag tacaaagtcg
     3181 ctcccagagt gagaatgcca gccgcggaga gaatatcgga ggtcaaccga taggcttgca
     3241 catccgattg caagtctttg gctcgatcac ttggcgttcc cacatactgt tccttttcca
     3301 ttttggaaga ggaactcaaa gccatggcgc cggtgactcc gccacccacg agcaaggctc
     3361 cccccacacc aagaccgatg tacgaaaggg ttgtccattg tgactcgggt tctttgatgc
     3421 gttcggtttc gcgaaccacc acccgggaag aacccgctgg ttccaattgc aagcgaatcg
     3481 tgcgtttttc ggcgcccgcc acttcgatgg cctgcgatat ggggatgtaa ccttccttcg
     3541 atacggccac ccgatgcgcc ccagcgctca acaaaatggt atcgagcggc gttttaccca
     3601 ccgggatgtc atcaatcacc acggaggccc cctcgacatt cacggcgata gagaccgtag
     3661 cgattcgggg cctgagtttg ctgatttcgt tttccacgct ttgacgacga tccgccgaga
     3721 cattgtttcc cccatctttg aggtatttct caaaagaagt gacggcgcaa acgtagtctt
     3781 gaagctgata acagacttgg gccacgttat agagcacgcg atactcgccc gtaatttcgt
     3841 aagattttcg aaactcggcg agagcgccgc ggtagttctc ctcgttatac agatcaagac
     3901 cacgacggta ccgggcggca gcctcggagg catcttgctc ttccggggca gtttgggcac
     3961 ctgccggtgt ggacaccacg agaggggaaa acagcaacaa gacgaacagt aggttcgatg
     4021 cgctaccggt acgcaggatc atgtccagct aacctcgctt tcactcgaag gggttgtcat
     4081 catccaattt ccgtttcgtt tttcccttac taggatctgg tgtggggacc ggaggcggag
     4141 tggccgtggt gggttccgca ggctccgcgg tcggcttggg cccccgagga tgaggaccca
     4201 ctacaggtcc aaccttagcc ggtggagccg ttgtcgacgc cgcggaagta tccgctggca
     4261 gctcgtgctt ctcgagttcg aattggatga cctgatctcg atcgaatcga atttgctccg
     4321 ccttacccac ataccctggc gcttccgcac gaatcacgtg cttctcgcca tccttcggta
     4381 ccgtcaactc catcggattg gtcgcgagcg ttttgccatc gagaaacatc cgagcaagac
     4441 tcggctgcgc cgatacttga aggtggatgg ttcccactgg ctgctcagcc gatgcacttg
     4501 ccgtcacagg tggcgtatcc gtggctgcat ccccggagtt caatccgatc accaaggccc
     4561 ccaccaacaa gagcccccca acccctacca cgacagccca cattttcgtt tttcgagccg
     4621 cgggtaattc cgtcggaacg gagaattcca cgtttcgatc catgccactg cgcgaagaac
     4681 tcgaagagtc aggccacgaa cccgacgcgg tcgccaaaga agccacagag aattcatccg
     4741 tgctggtaag cgatgccgcc gatagattcg cttcaatcag cgcacgagcc ttcgccctat
     4801 cctccgcaaa cacacgcatc atgtgggcgc taatctcttt gcgcccaatt ggctgcccca
     4861 acgacaagat gaattgatcg agatccgatt ggaattcaag agcatcctgg tatcgatcct
     4921 ccgggcgaaa cgcgatggct ttcatgcaga ttcgctcgag ttccggatga atatggggca
     4981 ccgcctcccg aatcggaggt gcccccaccg aagcgaccct cgtcatcacc gccacatccg
     5041 acattccttg gtatggacgc tgtttcgcga gcgcttccca caaacaacag cccaacgcat
     5101 aaatatccac ccgaaggtca agagaagctt ctcccaacaa ttgctcagga gccaaaaaag
     5161 acaccttgcc cttgatggtc cctgctcgcg tttgggtcga tacggagacg gctttggcga
     5221 taccgaaatc cagcatcttc acggtgccgt cgtaggtcac gaaaatgttg tgcggcgtga
     5281 catcccgatg aacgagttgt aggggcgtgc catcgtagtc cgtgcattca tgggcatgcg
     5341 caagacctgc caaggcttcg gacaaaatcc gcaacaacac cggcaaaggc accaattcct
     5401 cttcggactt gcgaatgcgc gacatcaatc gggacatggg ctgcccctgc aaatattcca
     5461 tcgcaatgaa tagacgcccg tcctcctcgc ccacttcctg aacactcacc acgttgggat
     5521 gactcaaacg cgcggccaaa cgcgcctcgt tgagaaacat ctcccttccc tctggctgct
     5581 cagcgaactg ctctctcagt attttgagcg cgatcaactt gctgaaccgc gcaggcccct
     5641 ccatgaccgc aagaaatacc tcggccatac caccgtggcc gatttccgcg atgaaccgga
     5701 acttccctaa tctgcgcggc ctagccatct cttgcttttt gacaccttcg acgtccgttg
     5761 tcacaaccta acgttcgcac atattgaacc gtaattccat cgtaatcaca aagccggggg
     5821 gctaaccttt ctccttattt tcaaggcaaa cccgttccgg acgccgcccc atcgcttccc
     5881 gaacaagaat attttctgaa cggtaatcat tcccgttatt tcatataaat tttataaaac
     5941 aatgaactaa cattatattt tcataaccaa cgcttacgac gaaaataggt aagaagtccc
     6001 acaaccacca aaaccattag cccccaaacg aataagtacc cccaacgcca ccgcaattca
     6061 ggcatgtact cgaagttcat cccgtacaca cccgcgatga atgacaaagg tagcatgatc
     6121 gtagagatga ttgcaagcac acgcatgatc tcgttggtcc gattggccgc cgacgacata
     6181 tacatctcta ccgaagcgtt cacgagttcc cgataggaat ccgcatggtc ggcaatccgc
     6241 acgaaatggt cataagcgtc acgaaaaaat ggtagagcat gctcgggaat caccccaaat
     6301 tcaccacgcg acagccgaag cagcacgtcg cgctgatacg tcgaaacacg acgcaacgcc
     6361 tgcaacgagc gtcgcaacgc aaataaccgc gtcaaaatat caggcaccgg ctcttcgatg
     6421 atctggcgac tcacctcgtc aatatcatcg tcgaacgctt cgatgaccgg ataataatca
     6481 tccgcaagcc gatcgatgat cgcatgggcc acatatgcag acccacgcaa aagagcacgg
     6541 gggttgcgct ccagctccgc tcgtaacccc tcgacggacc gcatggacct cggatgatgg
     6601 gtaaacaccc acgtcggtcc aatcaaaatg tcaatctcta ccgtccccaa cgactccgga
     6661 tcctgcgcct cccgatggat tccgtgaagc acaatataaa ggatatcgcc gtaatcctcc
     6721 accttgggcg tcggctgatc aaaaaagata tcctccaccg tcaaaggatg tatccccatc
     6781 tgctcttgca aaaaactctc gacctcggcc cggcgctcat cggtttctat ccacaccgta
     6841 cggtctttcg cccgtatcaa cgccggaacc tcggagaaat cccgaatgac gtagcggcct
     6901 tccgaatcaa ccagatgggc tcgtgtcacc atggcccgtc acttcccatc gctcgataca
     6961 accatccacg tcccatcgct tcgtgtctcg accccctcgc catgattcgt acgacccatg
     7021 gatcaccctt tctcttgctc cgaaaaccca tcatccaccc aaaccctacg gcgagttccg
     7081 aaggcgaatc aagccatcct cggacaaaat gaacctgctt tgcccccgta ttaccccaac
     7141 cccgctcgac cacctcgtca tcatgcacgt ttccgaaaca cccccacacc tcatctgttg
     7201 ctcgcggcgc gttggcgcta cactgtgatg ttggtgtgtg ttgtgccacg acgaaaggat
     7261 ccacgatgtc cccatcgctc cgcctcgcga gcgtttgctc cttatcgctt attttttcag
     7321 caacctgcgg tgccggttgt cagtccgcgg ctggcgattc caagccctac gtaagcacac
     7381 ccgaaaacga cgcctcccaa gatgaaccag acgccaatga actcgacgcg gtcgaggaac
     7441 cagaacaaga aacttcgttc atcgatccgg ataccggcac caaagaaagc ggtgtcgtgg
     7501 tcgtggaacc cccttgcgac aacgcaaacc tgtatggcga cgaagacggc gacggatggt
     7561 cggtggccga tggggattgc aacgattgta cgccgctgat gaaccccggc gcctacgatt
     7621 atcccggaag caaagtagac gaagactgca atggtatcgt ggacgacgag ccaaagggat
     7681 gcgacgaagg actgcccatc gaaggcaatg atcccatgga cgcagtacgc gccctggggt
     7741 tgtgccgaac cgccgatccc aacgctccca tggccaaaaa atcctggggc gttttatcgg
     7801 ctcgctatgt ctttccagat gggtccaccg cctcgcgcac cccgaaacaa tttggccccc
     7861 attgcgtagg cgacgggcaa aaggggactc ctcccaactc cttgtcacgg ggtatcttga
     7921 cccattttgg gaacgtgacc gtgccgaccg gaggcaaaac catgctcgcg ctgtccacgg
     7981 gggtcgcccg ttcgggctcg cagggaacct cacccgccgg cgcacaaatg tgtacgtcca
     8041 gccatactcc cccgggtttt cccaccccat cgaaagcagc ttgccccaat cagaaaatcg
     8101 acgaagacga caccgcttat gacgccatcg ccctggagat cgaaataaga accccaacga
     8161 atgccgaagg gttctctttc gacttcaact ttcataccta cgaatacccc aacttcattt
     8221 gctcgcaata caatgacttc ttcgtggctc tgttgtggcc cgtccatgcc acgaacgtgc
     8281 tgcataacaa catcagcttc gatccgcagg gcaatccagt gtccgtcaac aatggctttt
     8341 tggaagcctg cctacccgga acccacggcg gaaagacctt cccatgtcct cttggcatcg
     8401 atgaactcgc ggggaccggg ttcgacagcc gaggcgccac cggttggctt cgcaccaccg
     8461 cccccatcga acccggcgaa accatcaaac tccggttcgc catctgggat atgggcgatg
     8521 atgcctatga ctccaccgtg ctgctcgaca acttcacctg ggccgtcgaa agccttcccc
     8581 ccttcaccga tcgccccccc aaatgaacct cacgcatgat ctacgaatcc cgaggttccc
     8641 ccgactcatc gtccccatcg tccaccaccg catccgtcat gcccaatggc tctgccaaac
     8701 gctgctcgag ttgcggattg gcttcgaata ggcgttgggc ctgctcggcc agagtaccaa
     8761 aatccattgg aaaattagga agttgcgcca tatcgggcag cagctcttgc tccgcttggc
     8821 tcagacctgt cccctggccg tttccgatca agtgctgcgc gaaccgcatc gccttttcca
     8881 gcatttcggg cgaaacctgc tcctgcatct gcgccatcag cgacaccaga tccccctgaa
     8941 aatccaactc agaaccgaag tcccccagca tcacttccgc actttcgagg ggtgcccaat
     9001 cgagggcatc gcccatctct tccaacaacc ctgcccaggt tcgcgcacga ggttgatagg
     9061 tttctgggta caacgcaacc cctcgcggat cgtcatgctc ttcgacgaga tcaccgagaa
     9121 ccgccgccac gagcgctccc atcacctccg gctccgcata accaagcgca acctgcatcc
     9181 gaatgccggt cgcatcctcg aaagcatcga tgacatagct gttgccatcc gcaccttcca
     9241 ccgtctcgca cccagacccc tcgagcgctt gccgttggct gtccgatccc ggctccagca
     9301 tctccatcac acggctggg
//
LOCUS       DAOSOR010000180         9241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_7425_length_9241_cov_3.878837, whole genome shotgun sequence.
ACCESSION   DAOSOR010000180 DAOSOR010000000
VERSION     DAOSOR010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9241
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7425_length_9241_cov_3.878837"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PK710_11320"
     CDS             <1..320
                     /locus_tag="PK710_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10355.1"
                     /translation="YVGTAMENTARVELETALDSLRLTATTIAACTGTPPARDDLYFI
                     TVRLWTQLHGYIAGIHNHLIDYMHPDPASLKERMITRIKNSVVHEILALNPTAAREQP
                     TLQ"
     gene            336..1268
                     /locus_tag="PK710_11325"
     CDS             336..1268
                     /locus_tag="PK710_11325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOT10356.1"
                     /translation="MTAILTKHEPLLLEVKGNALDDGPGIRSVVFFKGCKLSCRWCHN
                     PESLSQNAELSFNVADCISGCTRCRGHCPEDAIMPGVGINRWACTRCFDCVPVCPSGA
                     LRQVGKPANVAELMAKLRADKPFYEASGGGVTFSGGEPTLAMELLGELAAACRAEGIH
                     TLLETAGYFRWEPFERGVLPHLNAIYYDLKLMDSEDHRAYCGVPNERILANFKRLVEL
                     SRAGTFDLQPRIPLVPDITATEENLTAIGRFLLDVGVRQVRLLDYNPTWVDKGPQLGI
                     ANKFTELDIMRTWLPRGLAARLRQHLVDLGLEAI"
     gene            1322..4372
                     /locus_tag="PK710_11330"
     CDS             1322..4372
                     /locus_tag="PK710_11330"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOT10357.1"
                     /translation="MAEPTAVKLPPVDPLDMRPFHPDPAVSDAARHSPQQLIVRGLLG
                     LMAKLYTWHPRLRDELRIKDGWIDLVVGIRTRSGSVRTGIRFRDGKAKAMGRIPEAVD
                     VVLVFKSDAEVTKFLTATPTEQVLMTLRNDFYVDGNTSSLNLFVYLISRLLFRQQIKN
                     LAKDLRNDNADRRTQAGATNPDLARQMRERADYRIATSQVDPGVKYLEDPYLRDFSLK
                     DFPRLAKFLNRHLDHDAEVCPELPKLLTEWHRKHGFERDSKGKPWHPVLRKALAYQYV
                     MENRRPIIHANDLIAGTTTTKETGVVVYPEGHGTMIWGELLTVPHRPLNPYGVSEETR
                     ELLHKDVFPYWSRRNFKEWVRDHYRSPLGQRIDDRFAVYFDWKTATISHTIPDFPKML
                     RLGTSGIIAEIEEAMAADSDEERNTTRRAFILALEGLGTYSRHLAAQAAYEAERESDP
                     SRAAELRRLAEICSQVVVGPARTLDEAVNSVWITWVGLHMESTNAGLSMGRLDQWLQP
                     YFEADMGKLETLEQRRAYTKRAIELVGDLMLRCTDHLPLTPDVANYYFGGSSSDQAIT
                     LGGVTPEGEDAVCDMTYIFLKVTEILSLRDPNVNARFHLGKNSQAYLKRLCEVNLITA
                     ATPSLHNDEVVGKALSRYGYEVRDVRDWAATGCVEPTLSGKHIGHTNFQMLSLVAALE
                     MALNDGWHPLMDWKVGPSTGSIEAGDFANFDQFFEAYRQQVAFLVDRSIEYNEMLGKA
                     HQQLRPTPLLSSLIEGTIASGKDVTYGGARYNSSGAACIGLVDVIDSMLVIQKLVFEQ
                     RVVSFADLKRAVDTNFANDPLLLAQVSHKVALFGSGNEDAVAMGNRITKMVQSLYDRY
                     TNYRGGRYAAGFWSMSTHVSYGTLAGALPSGRLAGKPFTPGLTPTPGASPNLLDNIRD
                     VARLDPANMPNNIAFNVKVVPSAHESRERTVDTMAAYVKSYFSLGGMQMQLNAVTSDT
                     LRDAMVHPENYRNLLVRISGYNAYFVTLNKQMQLELIERAEYGI"
     gene            4551..4760
                     /locus_tag="PK710_11335"
     CDS             4551..4760
                     /locus_tag="PK710_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10358.1"
                     /translation="MDRSLCDSAVDIGGSLEEDVEKRAACLGNHGMNLRALELGSGLA
                     VEGVAISRRFRSRPGHYGARSISPT"
     gene            complement(4730..6223)
                     /locus_tag="PK710_11340"
     CDS             complement(4730..6223)
                     /locus_tag="PK710_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10359.1"
                     /translation="MKRALCLAALVIVPPACAADRESHVSAGATNPVLDASIDPDDVS
                     DSYIPWDGSPEEPTPVDASDEIGGDSSIDSSKDAGEESAIEPFIPAELRVGLIHTGPS
                     PQTTPATWNAHLPKIVGDGAFWYAVHTHYPPSVADRYAAIMRREAGSALHAWVEVARI
                     AYPHQSPGIVMDISQRLHLIFDCMRPATQDVLCFPGGAGTTGRNSRFYHLIFSTRDGS
                     GALRFDTYSNHDEWTAESNGYQGIATTTDGVTVWALADSAWGRVVQWWYSGAQYGTIG
                     TLTAAPAYLLYPILTGHPSLGSSQLILYAGEFDPAGGNNASYLASTAYVGNTSSLSQL
                     FRRAPAQPVPGIVSAYPSDLEHDAQGTLYALSYLPKSEKQCTELLRFDSELAQPPTIV
                     PVGCVSNYAKLKFASHGALYLLDAGPGATISLGVSMDRGDSWTWHSVPITGLPSNGDI
                     RYVGYTPVKPYTSPLTYEPNRWVVFFSGLDESNGSVHSYVGEMDLAP"
     gene            6840..8414
                     /locus_tag="PK710_11345"
     CDS             6840..8414
                     /locus_tag="PK710_11345"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOT10360.1"
                     /translation="MSTSLDRRDLLQVMGYGAATVSLPFVAGCGRTEIQRLTSKSPSL
                     QEPGKDSGAVSVSHFDAFGVDEGLIRKVLSRALSRGASFSEIFLQHRVSHWVGMEDGE
                     VNRAYSEVGLGAGIRVIQGDTTGFSFTEDLTPGALLEAADMASAVADGPSSPRSITLT
                     PRIVVSRYTVETPWTEVGIDRKLPIVQRAEKAARNHDTRIIKVTVALQDETSHILIAN
                     SEGLLVADERPMGVLSVSCVGENKGKTETGYWTFAQRDGIRFLTPAHIDDTGTTAARN
                     TVLLFEAVSPPVGDMPVVLGPGNPGLLLHEAIGHGMEADFNRKGISIYANRIGKRIAP
                     EGVTIVDGGTGRQRGSLNIDDEGHPTEETVLVENGVLRTYLHDRISAQHFKAPRTGSG
                     RRESYCYPPVPRMRNTYMLAGKESPEEIIASVKKGIYAEIFTNGQVMIGAGDFTFYLK
                     HGRLIEDGKLTHVIKDANLIGNGPNVLEKVAMIGNDLKHITGAGYCGKDGQGVPVGFG
                     LPTLKVSGISVGGRGA"
     gene            8411..>9241
                     /locus_tag="PK710_11350"
     CDS             8411..>9241
                     /locus_tag="PK710_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10361.1"
                     /translation="MNATLMQLVENALILAKRQGAAAARASVWRMRESGVEWRDGKLD
                     RLRESVHKGLSISLFVDGRYSSHTTSDLRPEAMELFVAESVAMTKVLQPDPHRKLPPL
                     ERCQERFEGDLLLLDRDNLGVIDGAAQKKQAMELERAMRQAEGADLILSATGAMRVYH
                     TMSAMRDSQGTKGERESSVFARSASATVRGEGDRKPSGGDYAATHHYAELPSMDLMGK
                     EAVRRALAGIGETPWKSGKYPCIIENRVADRLLGGLLGPLGGNSLQQKRSYMSGKLGE
                     K"
BASE COUNT         1920 a         2410 c         2922 g         1989 t
ORIGIN      
        1 actacgtggg cacggcgatg gagaacacgg ctcgggtcga actcgagacg gcgctcgaca
       61 gtctccgcct gactgccact accatcgctg cctgcaccgg cacgccgcca gcaagggatg
      121 acctgtactt catcacggtt cgcctgtgga cgcagttaca tggttatatc gcaggcatcc
      181 acaaccacct catcgactac atgcatcctg acccggcttc tctgaaagag cgcatgatca
      241 cacggatcaa aaatagcgtt gtccatgaga ttctcgctct gaaccccacc gctgcgcgtg
      301 agcagccaac cctgcagtga ggagggatag cagcgatgac ggccattctg acgaagcacg
      361 aaccgttgct gctcgaggtg aaaggcaacg cgcttgacga cggtccgggc attcgctcgg
      421 tggttttttt caagggctgt aagctgtcgt gccgatggtg tcataacccg gaaagtttga
      481 gtcaaaacgc tgagttgtca tttaatgttg cggattgtat ttccggctgt acccgatgtc
      541 gtggccactg ccccgaggac gccatcatgc ctggtgtcgg catcaatcgt tgggcctgca
      601 ccagatgctt cgactgcgta ccggtctgtc catcgggggc tttgcgccag gtgggtaagc
      661 cagccaacgt cgccgagttg atggccaagc ttcgcgcgga taagcccttt tatgaagcct
      721 ccggaggcgg cgtcaccttt tccgggggag agccgactct agcgatggag ctgcttgggg
      781 aacttgccgc ggcctgtcgt gccgaaggta tacacaccct tttagagact gccggatact
      841 ttcggtggga gccgttcgaa cgaggggtgc tgccgcatct gaacgccatc tattatgacc
      901 tgaaactcat ggattccgag gaccaccgtg cgtattgcgg ggtgccgaac gagcggatcc
      961 tggccaattt caagcggttg gtggagctat cccgtgccgg gacgttcgat ctgcagccgc
     1021 gcattccgct ggtcccggat atcaccgcga cggaagaaaa cctgacggcg atcggccgat
     1081 ttctgctcga tgtgggggtt cgtcaagtcc ggttgctgga ttacaatcca acctgggtag
     1141 acaagggtcc tcaattggga attgctaaca aattcactga gttagatatt atgcggacat
     1201 ggctgccccg cggcctggca gcccgactgc gccaacatct cgttgatctc ggcctggagg
     1261 cgatatgacg ttccccaaca cctcttcgtc cacttcttcc ggaaccgagc atgccatgac
     1321 catggccgaa ccgaccgctg tgaagctgcc cccggtggac ccgctggaca tgcgcccatt
     1381 tcatccggac ccggcggtga gcgatgcggc gcgccacagt ccgcaacaat tgatcgtgcg
     1441 tgggctgctc ggtctcatgg ccaagctcta cacctggcac cccaggttgc gtgacgaatt
     1501 gcgtatcaag gacggttgga tcgatcttgt ggtcggcatt cgcacccgct ctggctccgt
     1561 gcgcaccgga atccggtttc gagatggcaa agcaaaggcg atgggccgca ttcccgaggc
     1621 cgtcgatgtc gtgctggtat tcaaatccga cgctgaagtt acgaaattcc tcacggccac
     1681 gccaaccgag caggtcctca tgacgttgcg caacgacttt tacgtcgatg gcaatacatc
     1741 atcgctaaac ctgttcgtat atctgatttc ccgactcctt ttccgccagc aaatcaagaa
     1801 tctggcgaaa gatcttcgta acgataacgc tgatcgtcgc acacaagccg gtgccacgaa
     1861 tcctgatctg gcccggcaga tgcgcgagcg tgcggactac cgcattgcga cttcgcaagt
     1921 cgatccgggt gtgaaatatc tcgaggaccc ctacttgcgt gatttcagtt tgaaggattt
     1981 cccgcggcta gctaagtttt tgaaccggca cctcgaccat gacgccgagg tatgcccaga
     2041 attgccgaaa ctattgaccg aatggcatcg aaaacacggt ttcgagcgag attcgaaggg
     2101 caagccctgg cacccggtgc tgcgcaaagc cctggcctat caatatgtga tggagaatcg
     2161 tcggccgatc atccatgcca acgacctcat tgccggcacg acaactacca aggaaaccgg
     2221 tgtggttgtc tatccggagg ggcatgggac gatgatctgg ggcgagctgc tgacggtgcc
     2281 gcatcgacct ctgaatcctt acggtgtctc ggaggagacc cgagaattgc tgcataagga
     2341 tgtattcccc tattggagcc gtcgcaactt caaggaatgg gtgcgagacc actaccgttc
     2401 gcctttgggc cagcgtatcg acgaccgatt tgcggtctat ttcgactgga aaaccgcaac
     2461 catctcacac acgatcccgg atttccccaa gatgttgcgg ctgggaacgt ccggcatcat
     2521 cgccgagatc gaggaagcga tggctgccga ctcggatgag gagcgtaata ccacccggcg
     2581 ggcattcatt ctcgcgctgg aggggttggg cacctattct cggcatttgg cggcgcaagc
     2641 ggcgtatgag gcggagcggg aaagcgaccc cagtcgggcg gctgagttgc gccgactggc
     2701 cgagatctgt agccaggtgg tcgtggggcc agcccgcacg ctcgatgagg cggtgaattc
     2761 ggtgtggatc acgtgggtgg ggctgcatat ggaaagcacc aatgccgggc tgtcgatggg
     2821 ccggttggac cagtggctgc agccatattt cgaggccgat atgggcaagt tggagacttt
     2881 ggagcagcgg cgggcctaca ccaaacgtgc gattgaactg gtgggtgact tgatgctgcg
     2941 ctgcacggac cacttgccgc tgactcccga cgtggccaac tactactttg gtggcagttc
     3001 ctcggatcaa gcgatcacgc tgggcggggt gacccccgaa ggcgaagacg cggtctgcga
     3061 tatgacgtat atattcttga aagtcactga aatccttagt cttcgcgacc caaatgtgaa
     3121 cgctagattc catctgggca aaaacagcca agcgtacctg aagcgtcttt gcgaggtcaa
     3181 tctgatcacg gcggcgactc cgtcgttgca taacgacgag gtcgtgggga aggccttgag
     3241 ccgctatggc tatgaggtac gtgacgtgcg tgattgggcg gcgaccgggt gtgtggagcc
     3301 gacgctgtcg ggaaagcata tcggccatac caatttccag atgctcagtc tggtggcggc
     3361 gctggagatg gcgttgaacg acggctggca tccgctgatg gattggaagg tgggaccatc
     3421 taccggttcg atcgaggctg gcgattttgc gaatttcgac cagtttttcg aggcttaccg
     3481 gcagcaggtg gcttttctgg tcgaccgatc gatcgagtac aacgagatgt tgggtaaggc
     3541 gcatcagcag cttcgtccga cgccactgct gtccagcctg atcgagggca ccattgcatc
     3601 gggaaaggat gttacgtatg ggggagccag gtacaacagt tctggggctg cgtgcatcgg
     3661 gctggttgac gtcatcgatt cgatgttggt gatccagaag ctcgtattcg agcaacgagt
     3721 tgtgtcattc gctgacctca agcgggcagt ggacacgaat ttcgccaacg atccgctgtt
     3781 attggcgcag gtatcccaca aagtagcgct ttttggctcg ggcaacgaag acgccgtggc
     3841 gatgggcaac cgcattacca agatggtgca gtcgctgtat gatcggtata ccaactaccg
     3901 gggaggccgt tatgcggctg ggttctggtc gatgtctacg catgtatctt acggcacgct
     3961 ggctggagct ttgccgtcgg ggcggttggc gggcaagcca tttacgccag gtctgactcc
     4021 cacaccgggg gcatcgccga acctattgga caatatccgt gatgtggcca ggttggatcc
     4081 ggccaatatg ccgaacaata ttgccttcaa tgtgaaggtg gtgccatcgg cccatgagtc
     4141 gcgggagcgg acggtcgaca cgatggccgc gtacgtgaag tcctattttt cgctgggggg
     4201 catgcaaatg cagctcaacg cagtcacgtc cgataccctt cgcgacgcga tggttcatcc
     4261 ggagaactat cgcaatttgc tcgtgcggat ttccgggtat aatgcctatt tcgtcacgct
     4321 gaacaagcag atgcagctcg agctgatcga gcgcgctgag tacggtatct aggccaacgc
     4381 gggtaagttc cagcgatctt tcctgctatg gtcctgtgat aatggcaccg cgggtggacg
     4441 tttttcgaca gggaggtctg cgaagcagtc ccgataagga cattggaatg agcgagatac
     4501 gacagatttg tcacatcccg gctgctcgtg cgcgggggag acgagctttc atggatcgta
     4561 gtctatgcga tagtgcggtg gatattgggg ggagccttga ggaggatgtt gaaaaacgag
     4621 cggcttgcct tggaaatcat gggatgaatc ttcgcgcttt ggagcttggc agcggtcttg
     4681 ctgtcgaggg ggtggccatc tcgcgacgct tccgttcgcg gcccgggcat tacggcgcca
     4741 gatccatctc gccgacgtag ctatgcaccg atccgttcga ctcatcgagt ccggagaaaa
     4801 acaccaccca tcgatttggt tcgtacgtaa gtggcgaggt gtagggctta accggcgtat
     4861 atcccacgta gcgaatatca ccgttggatg gcaatcccgt gatgggaacc gaatgccatg
     4921 tccagctatc gccccggtcc atgctcacgc caagggatat agtcgcgccg gggccggcgt
     4981 cgagcaggta tagcgctcca tggctagcga atttgagctt ggcgtagttg cttacgcatc
     5041 ccactggcac gatcgtgggc ggctgcgcga gctcgctgtc gaagcgaagg agttccgtgc
     5101 attgtttttc acttttcggt aaataggaga gcgcgtacaa cgtgccctgc gcgtcgtgtt
     5161 cgaggtccga gggataggcg ctgactatgc ctggaacggg ttgcgctggc gcacgacgga
     5221 ataattggct cagcgacgac gtatttccaa cgtaggcggt ggaagcgagg tagcttgcgt
     5281 tattgccccc cgcggggtcg aactccccgg cgtaaaggat gagttgtgaa gaaccgaggg
     5341 acgggtgtcc ggtcaagatt ggataaagca ggtatgcggg ggctgctgtc aacgtgccga
     5401 tggtgccgta ttgcgcaccg ctgtaccacc actgtaccac acgtccccag gcagagtcgg
     5461 ccagtgccca tactgtcacc ccgtcggtgg tggttgcgat tccctggtag ccgttggact
     5521 ccgcggtcca ttcgtcatgg ttgctgtagg tatcgaaacg gagcgcgccg ctgccatccc
     5581 gcgtggagaa gatcaggtgg tagaatcgcg agtttcttcc cgtcgtcccc gctcctcctg
     5641 gaaaacagag cacatcttgc gtggcggggc gcatacaatc gaagatgagg tgcagccgct
     5701 gcgatatgtc catcacgatc ccaggggact gatgcggata agcgatacgc gcaacttcca
     5761 cccaagcatg gagggcgctt ccggcctcgc gtctcatgat tgccgcataa cgatccgcta
     5821 cggagggggg ataatgggta tggaccgcat accagaatgc gccgtcgccc acgattttcg
     5881 gcaggtgggc attccatgtg gcaggtgtcg tctgggggga aggtccggtg tggatcagcc
     5941 ccacccgcaa ttcggccggg atgaacggct cgatggcgct ttcctccccg gcatccttag
     6001 acgagtcgat cgatgaatcc ccgccgattt catcggaagc atcgactggg gtgggctctt
     6061 cgggcgaacc atcccatgga atataggaat cagaaacatc atccgggtcg atgctggcgt
     6121 cgagcacagg attggtcgct ccggcggaaa cgtggctctc gcggtccgcg gcgcaagcgg
     6181 gtgggacaat gacgagcgcc gcgaggcaaa gcgcgcgttt cattctccaa ggatacagtg
     6241 atgcggtgat gggagcaagg tcgagttgtg cgggagtgga gagttccggc gactatctgg
     6301 ttgcaagaca acccgatgac gcggtttcaa agcgaatgag cgggacaggc tttcgtttcc
     6361 gcaaggttga acgcatcggc atgggtgatt aatgtgtaag caatggaaat aattgatgaa
     6421 tatgaatctg tgctcgtcca tggctcggga gtcttgtggc gtgggagtat ccggctatcg
     6481 acgccgctgc ggggaaaaac ctcggcatcg ccgacagagt cgtgatggcg caacacgagc
     6541 ccgccctggt cgagatccaa cctgtcgaaa aggctcaaca aggaagtatg tcaatgcggt
     6601 gaactcgtta tgatgcagta tcgacgtggt gggcgcttgg ggctgcagcg gtcgttttgg
     6661 gtgtagtcaa ccaccatggg aacgcgtcaa gggggtgttg cgttggcagg gcacatgctc
     6721 gaaaaagcat ggaagtcccg gcgggatcgg ccaagatgac gggtggagac tttttccgtt
     6781 ggcgtcttcg aaggttctcc ggtagaccag ggaaaaccct tggaaaatga ggcaaatcca
     6841 tgtccacttc tctggatcgc agggacttat tgcaagtcat gggctacggt gcggctaccg
     6901 tatcgctgcc tttcgtggcg ggttgtggtc gcacggaaat ccagcgtttg acttccaagt
     6961 caccgagttt gcaggaaccg ggcaaggatt cgggagcggt gagcgtgagt cattttgatg
     7021 ctttcggtgt cgatgaggga ttgatacgca aggtattatc ccgggcgttg tcgcgggggg
     7081 cttctttttc ggaaatattt ttgcagcaca gggtatcgca ttgggtgggt atggaagatg
     7141 gcgaggtcaa tagggcctat tccgaggtgg gtcttggcgc gggcattcgc gtgattcaag
     7201 gggatacgac cggattttca tttacggagg acctgacgcc cggggccttg ctcgaagccg
     7261 cggacatggc ttccgcggtg gccgatggcc cttccagccc gcgttcgata accctcacgc
     7321 ctcgtatcgt cgtgtctcgc tacaccgtgg aaacgccgtg gaccgaagtc ggcatcgacc
     7381 gcaagcttcc gatcgttcag cgggcggaaa aagcggcgcg aaatcatgat acgcgaatca
     7441 tcaaggtgac ggtggctctt caagatgaga cctcccacat cctgatcgcc aattccgagg
     7501 ggctgctggt cgccgacgag cggcccatgg gcgtcctttc cgtgtcctgt gtaggagaaa
     7561 acaagggaaa aactgagacg gggtactgga ccttcgccca gcgcgatggg attcgttttc
     7621 tcacgccagc tcatatcgac gacacgggaa ccacggcagc gcgcaatacg gtgttgcttt
     7681 ttgaagccgt gtctccgccg gtgggagaca tgccggtcgt ccttgggcca ggaaatccag
     7741 gactgctctt gcacgaggcc attgggcacg ggatggaagc ggatttcaat cgcaagggga
     7801 tttcgattta tgcgaaccgg attggaaaaa gaatcgctcc ggagggtgtg accatcgtgg
     7861 atgggggcac tggacgacag cgtggttcgc tcaacatcga cgatgaaggg catcccaccg
     7921 aggagacggt attggtggaa aacggcgtat tacgcaccta tttgcacgac cgtatttcag
     7981 cgcagcactt caaggcgccc cgtacaggct cgggacgtcg tgaatcctat tgttatccac
     8041 cggtgccgcg aatgcgcaac acttatatgc ttgcggggaa agaaagcccg gaggagatca
     8101 tcgcttccgt caaaaagggg atttacgccg agatcttcac caacggacag gtgatgattg
     8161 gtgccgggga ttttaccttc tatttgaaac acggaaggct catcgaagat gggaaactga
     8221 cccatgtgat caaggatgcg aacctcatcg gcaatggccc aaacgttctc gagaaggtgg
     8281 cgatgatcgg caacgatttg aagcatatca ccggggctgg ctattgtggc aaggatgggc
     8341 agggcgttcc ggtgggtttt ggattaccga ccttaaaagt gagtggtatt tccgtgggtg
     8401 ggaggggagc atgaacgcga cgcttatgca acttgttgaa aatgcgctta ttttggcgaa
     8461 acggcagggc gcagcggcgg cgcgggcaag cgtatggcgc atgcgggaaa gtggtgtgga
     8521 gtggcgcgat gggaagctcg accgcttgcg tgagtctgtc cacaagggcc tttccatctc
     8581 cttatttgtc gatgggcgtt attcttccca cacgacaagc gatttgcgtc cggaagccat
     8641 ggagcttttc gtggccgagt cggtggcgat gaccaaggtg cttcagccgg atccccatcg
     8701 caagcttccc cctttggagc gctgccaaga gaggtttgag ggcgatcttc tgctgttgga
     8761 cagggacaat cttggtgtca tcgacggtgc ggcgcaaaaa aagcaggcga tggagctgga
     8821 acgagcgatg cggcaggccg agggcgcgga tctcatcctt tcggctacgg gagcgatgcg
     8881 tgtctaccac acgatgagcg cgatgaggga tagtcagggc acgaaaggcg agcgggaatc
     8941 ttcggttttc gctcgctcgg cttccgctac ggttcgaggc gaaggggatc gaaaaccttc
     9001 cgggggagac tacgctgcta ctcatcatta tgccgaactt ccttcgatgg acctcatggg
     9061 caaagaagcg gtgcgaaggg cgttggcggg gattggagaa accccttgga agagtggtaa
     9121 gtatccttgt atcattgaga accgggtggc ggatcgtttg ctcggggggc tattggggcc
     9181 gttggggggc aactccttgc agcaaaaacg ctcgtatatg tcgggtaaac tcggagaaaa
     9241 g
//
LOCUS       DAOSOR010000181         9214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_7454_length_9214_cov_4.588711, whole genome shotgun sequence.
ACCESSION   DAOSOR010000181 DAOSOR010000000
VERSION     DAOSOR010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9214
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7454_length_9214_cov_4.588711"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3257)
                     /locus_tag="PK710_11355"
     CDS             complement(<1..3257)
                     /locus_tag="PK710_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10362.1"
                     /translation="MPRWFKRILGTFALMTVAACSGATGESCSGMQPLPAGFDPALRI
                     ENAASVRVTEAGFHFFETHLAPLATQLLTTMPNVENGVLSYPIERVDLNPGAGTNITV
                     CIDGPNEATDTCIAEIDIGSSSFSLSSANPSHIVAKGTVPLRLKVLPIEGTVLWIPGL
                     GVKGALSGGGKNDCNPNTMTFAKIPLQVAISIQVERNQNHKARVGYSKLSISEVNIDE
                     NEMTNALHFCGGGLDDDTLNALKPLVGGMVIGGFRDTVVETMNKKLCLATDPTKTPSC
                     PDGTTDKDGICTYADGACASMMLGLDGHVDLGALLASFSPGTKGALDVLFAVGGPGAR
                     SDDPASSWGDLNPVANGATLGMFGGVAPRPVSNCVTPVELTLPTGIPQPNELFGNAVP
                     GWTGDGPHVGFALSERFVNYALGAAYNSGLLCLGISTEQVGMLSSGLFSLIIPSFPYL
                     THQKQSASVALVVRPQQPPTITLGAGSDIKTDPLFRVALPQAVIDFYVWSSDRFVRAF
                     TVQFDLDVPINLTVSAEGLTPVLDKIYVNNAVVTNTDLLKDDPAMIASSLANLVEGLA
                     GDFLGNIPAFQLSDMLASFGLTLDLQQAGIKKLHKGNDNFLGVFAGLGIAPAITSQTS
                     STSVEILEKFTTYEGFQLVASSPSQRPRIVVQAYSSLDHGSNSVEYSYKVDRGLWHSW
                     RTSSRMLIDDPILMLQGNHTIAVKSRLVDQPRTEDPSPVLIDVRLDVLPPRIDIDWNE
                     DGTLRIDGSDIVSSRGSLVARIGFDDRDYGAWSSVEALASVQVPKGATMARVEMKDEE
                     GNVASKKQALIRGRASKIASSSEGCGCMVPRPGGWSQTVAWLVGLSLFGVGLRRHGRP
                     IRRALASVAITVLAGSWVGCSCSDDTETQEGKPDSGTPVCGSEGMDPCVVLEPGLVGA
                     YTSADVAPDGTLWVSGYNEADWEGNASYGDLVVGRWNGSSVDWVHVDGVPDEEVDKSV
                     YDVESWRGGLDSPGLDVGLWTSLKVDGAGLPRVAYFNLTNKSLEFASFDGTEWTVSNV
                     FRKADTEAGRYAKLLMVGGVPTIAFQLLEPGKDGYITSRVVLAHANSATPTGPGD"
     gene            complement(3416..4447)
                     /locus_tag="PK710_11360"
     CDS             complement(3416..4447)
                     /locus_tag="PK710_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10363.1"
                     /translation="MRTKLTIMTSLAVSTTLAMLMLAACGEEDKDGAGGPSTNTCWPS
                     GPGCGLDGVGRACMAWHDNAGSSTLSFRMSQLEVIKPDVLAGQFLQDNVVTKGVALNL
                     PQCFQEGSGRFSWLLEFDTANQRVKTGGAHVQLNPANGYCYINQVVEGFQTQPMEVPL
                     KLSNEGGETLFELSETIKDITVAIYLTDDESSAIILPLHEVMMSEGRISSDGNCIGAW
                     DGDHLYFDNDCKPDRNEGQKMQWVNGARLTGYIELEESDDVWIPEMTQTLCVALSGSA
                     LTYGEDFKEGGREGKRCARDDSGKIKAAEKADWCAASNTACSQPEADAFRLEGKFAAS
                     AVKVLASCP"
     gene            complement(4613..5212)
                     /locus_tag="PK710_11365"
     CDS             complement(4613..5212)
                     /locus_tag="PK710_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10364.1"
                     /translation="MNKLGCLWLGTVWLMACAPVENDNTDPWREVTAAPVVKPSCPAG
                     ERESVSLGNPFILVAQTVVETSSDHAPCSSFPHVWLMLESDACDVVLRSWHASMPCDA
                     GRFEALTSVTASTGAVREASASHVSSVHRVPIQGQDADGISEFVVDLTVDLSITPDRV
                     AIVQGAVGIPWENDALRFQAVGRLDDTSDAVPSRRLGCW"
     gene            5406..6065
                     /locus_tag="PK710_11370"
     CDS             5406..6065
                     /locus_tag="PK710_11370"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOT10365.1"
                     /translation="MFHSIIEHVFDPAWNAAHRVVINVGIVVILFLSGLGLPLPEDIP
                     LSAAGFTTFKQAGDEFVWWRYGLTFAMVVVPILLGDMCAWTLGRKYGLPIRDRFKILR
                     RVIDDKRVARVRSWFERYGGAAVFLGRQLAGVRFVTFFMAGVMRVTLPRFVFWDFIGC
                     VVSVPIWLTLGTLAARYGREWLLKASHTVGHTFLLIVLGAILILFLVTKLRARRENSN
                     T"
     gene            6154..6405
                     /locus_tag="PK710_11375"
     CDS             6154..6405
                     /locus_tag="PK710_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10366.1"
                     /translation="MNRLTPSAPTPSDPIDLGTTDELPAMGDWVEDTSVEPRDSAHDT
                     VPSPPPDGEYARITLPTVPSVGKLLDFLDEEATDPGSVG"
     gene            complement(6412..>9214)
                     /gene="metH"
                     /locus_tag="PK710_11380"
     CDS             complement(6412..>9214)
                     /gene="metH"
                     /locus_tag="PK710_11380"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOT10367.1"
                     /translation="FAEDGLLNIVGGCCGTTPDHIRAIGEAMKGLSPRVVPEHDGKTR
                     YAGLETLTVDETSNFIMIGERTNVTGSRRFAKLIMKAEYSRALEVALEQSRGGANILD
                     VNMDEAMLDAEAAMSTFLTWVASEPEIARLPMMIDSSKWSVIEAGLRCHQGKAIVNSL
                     SLKEGEESFVERARQVRRFGAAVVVMAFDEQGQADTADRKVEICSRAYRLLTERVGFP
                     PEDIIFDPNVFAVATGIEEHNRYAMEFIEAVRRIKAELPKAKVSGGISNLSFSFRGNE
                     PVREAFHAAFLYHAIRAGLDMGIVNAGQLAVYEDIPEELLRRVEDVLFDRRADSTDRM
                     IELAERYKGTGAKRSIDTSWREASVEKRIEHALVLGIVDHIEADVEEARVKYGRPLKV
                     IEGPMMDGMNRVGDLFGAGKMFLPQVVKSARVMKKGVAYLQPFLEAEKDDAQASRSKA
                     KLVLATVKGDVHDIGKNIVAVVLGCNNYEVVDLGVMVPANRIVEAAITHKADMIGLSG
                     LITPSLDEMVTVAKEMQRRELQIPLLIGGATTSRQHTAVKIAPAHQGVVAHVLDASRA
                     VGVVSQLRDPVQRRKFTEETFSEQDRLREIYGAKATRPMLSLQQARDRRKVIEFRAED
                     LARPDFIGRRVIEDVPIAQIEPFIDWTFFFLAWEMKASFPGILNSTRYGKAARELLDH
                     GKEMLRQMHEQRSVRCRAVYGFWPAHADGDDIVVYTDETRREEKLRFLMLRQQREKSR
                     AEEPYLCLSDFLTKVGSEQPDYVGAFAVTAGIGAEELVQRYEREHDDYRAIMTKALTD
                     RLAEALAEKLHAQARRDWGYGVGENFTNEDLIAERYRGIRPAFGYPACPDHTEKEKLF
                     SLLEASEIGVSLTDSFMMSPGASVSGIYLAHPAATYFSVGRIGKDQVQDYAKRKSTSV
                     TEVEKWLGSRLSYDPEDRR"
BASE COUNT         2007 a         2821 c         2403 g         1983 t
ORIGIN      
        1 caatcccccg gacccgtcgg agtggcggaa ttggcatgcg caagaaccac ccgcgaggtg
       61 atgtacccat ctttccccgg ttcaagcagt tggaacgcaa tcgtgggaac accaccaacc
      121 atcagtagct tcgcatatcg tcccgcttcc gtatcggctt tccgaaagac attggatacc
      181 gtccactcgg ttccgtcgaa cgatgcaaac tccagggatt tattggttaa attgaagtac
      241 gccacacgcg gaagacccgc gccatccacc ttcaagctcg tccataaccc cacgtccagc
      301 cccggcgaat cgagcccccc acgccaagac tccacatcgt agaccgactt gtccacttct
      361 tcatccggaa caccatcgac atgaacccaa tcgaccgaag acccattcca gcggccgacc
      421 acaaggtcgc cgtaggaggc gtttccttcc caatccgctt cgttataccc ggaaacccaa
      481 agcgtgccgt caggagccac atccgcagac gtataagcgc ccacgagccc aggctcgaga
      541 acgacacacg gatccatccc ctccgagccg cagactggcg ttcccgaatc cggttttccc
      601 tcttgcgttt ccgtatcatc gctgcaactg caccccaccc aggatcccgc cagcaccgtg
      661 attgccacac tcgccaacgc acggcgaatc gggcgcccgt gccgacgcaa gcccacgcca
      721 aacaagctga gtccaacgag ccaggccacg gtttggctcc agcctccggg ccttggcacc
      781 atgcatccgc aaccctcgga agaagaggcg atcttgctcg cacgaccccg aatcagcgct
      841 tgttttttcg aggcaacatt gccctcctcg tctttcattt ccacacgtgc catcgtggct
      901 cccttcggaa cttgcaccga agccaacgct tccacggaac tccaggcccc ataatccctg
      961 tcgtcaaatc caatccgcgc aaccaacgag ccacggctcg atacgatatc cgagccgtcg
     1021 atccgcaacg tcccatcctc gttccaatcg atgtcgatgc gaggaggcaa aacatccagc
     1081 cgcacatcga tgagaaccgg agaaggatct tccgttcgag gctggtcgac gagccgggac
     1141 ttgaccgcga tcgtatgatt tccctgcaac atgagaatcg gatcgtcgat gagcattcgc
     1201 gaagaggtgc gccagctatg ccaaaggcca cggtcgactt tgtaggaata ctcgacggaa
     1261 ttcgagccgt gatcgaggga agaataggct tgcacgacga tccgaggtcg ttgggagggg
     1321 cttgaagcca caagctgaaa cccttcgtaa gtagtgaatt tttcaagtat ttctacggac
     1381 gtactggagg tttgggaggt gatggctggc gcgatgccga gcccggcaaa cacaccgagg
     1441 aagttgtcgt tacccttatg tagttttttg attcccgctt gttgcaagtc caaggtcaat
     1501 ccaaaactcg ccaacatgtc agagagttgg aaggccggga tattgccaag aaaatctcct
     1561 gcgaggccct cgacaaggtt ggcgagggaa gaggcgatca tggccggatc gtctttgagc
     1621 aggtccgtgt tggtgaccac ggcgttgttg acgtaaatct tgtcgagaac gggtgtgagt
     1681 ccttcggcgg agacggtcag gttgatgggc acatccaggt cgaactgtac ggtaaatgcc
     1741 ctgacgaagc ggtcggaaga ccatacataa aaatcgatca cggcttgcgg aagcgccaca
     1801 cggaacaggg gatcggtttt gatatcggag cccgctccca aggtgatggt gggtggttgt
     1861 tggggacgga cgacaagcgc cacggatgcg ctttgttttt ggtgggttag gtaggggaag
     1921 gaggggatga tgaggctgaa caagcctgag gaaagcatgc cgacttgctc ggtggaaatt
     1981 ccgagacaga gcaaaccgct attgtacgcg gcacccaagg catagttgac gaaccgctcg
     2041 gacaaggcaa aaccgacatg aggaccatca ccagtccatc cgggaaccgc attgccaaaa
     2101 agctcattgg gctgcggaat gcccgtggga agcgtcaatt ccacgggtgt gacacaattg
     2161 gaaacaggcc gaggcgcgac gcctccgaac attcccaagg ttgcgccgtt ggccacggga
     2221 ttgagatcgc cccatgaaga tgcgggatca tcgcttcgcg cccctggccc tcccaccgca
     2281 aacaaaacgt ccagcgcgcc tttcgttccc ggtgagaacg acgcgagcaa ggccccaaga
     2341 tcgacatggc catcgaggcc tagcatcatc gacgcacacg ccccatccgc ataggtgcaa
     2401 atgccgtcct tgtctgtcgt tccatcgggg caggaagggg ttttggtggg gtccgtggcg
     2461 aggcatagct ttttgttcat cgtttcgacg acggtgtccc tgaagccacc gataaccatt
     2521 cccccgacga gaggtttgag cgcgttgagc gtgtcatcat cgaggccgcc tccacagaaa
     2581 tggagcgcgt tggtcatttc gttttcgtcg atattcactt cggagatgct gagtttcgaa
     2641 tagccaacac gagctttgtg gttttggttt cgctcgactt gaatggagat cgcgacctgc
     2701 agagggatct tggcaaacgt catggtgttg gggttgcaat cgttcttgcc gccgcccgaa
     2761 agcgctcctt tgacaccgag gccgggtatc caaagaaccg ttccttcgat cggaaggact
     2821 ttgagacgca ggggaaccgt tcctttggca acgatatggg agggattggc ggacgaaagg
     2881 ctgaacgagg aggagccgat atcgatttcg gcgatacacg tatccgtggc ctcgttgggc
     2941 ccatcgatgc aaacggtgat gttggtgccc gcgccggggt tcaagtctac ccgttcgatg
     3001 ggataggaaa gcacgccgtt ttctacattg ggcatggtag tcaacaattg cgtggccagg
     3061 ggtgcgagat gggtttcaaa aaaatgaaat ccagcttcgg tcacacgcac cgaagccgca
     3121 ttttcgatgc gaagagcagg atcgaacccc gccggaagcg gctgcatacc agaacagctt
     3181 tctccggtag ctcccgagca tgcggccacg gtcataaggg caaaagttcc aagaatacgc
     3241 ttgaaccaac gaggcatggt acctcccatc ctacgcgaca ccgaacgact atcctgacac
     3301 tatcctgacg aagtgtagct gcctggtcac caggggcgca acgccccccg acaacgaaca
     3361 cggagccatg ccctgtgttc gaagaaaatc aacgccaaca ttttcggcat tcgcgtcaag
     3421 ggcatgaagc aagcactttc acagcgctcg ccgcaaactt tccctcgaga cgaaaggcgt
     3481 cggcctccgg ttgtgagcag gccgtattcg atgcggcgca ccaatctgcc ttttccgctg
     3541 ctttgatctt gccgctatcg tctcgcgcgc atcgttttcc ctcacgcccg ccttctttga
     3601 agtcctcccc atacgtcagc gcactgcccg aaagcgcaac acataacgtt tgcgtcattt
     3661 ccggaatcca tacgtcgtcg gattcctcga gttcgatgta ccccgtgagc ctcgcgccgt
     3721 tgacccactg catcttctgc ccctcgttgc ggtccggttt gcagtcgtta tcaaaataca
     3781 gatggtcccc atcccaggca ccaatgcaat tgccatccga cgatatccta ccctccgaca
     3841 tcatcacctc atgaagaggc aagatgatcg cgctggattc atcatcggta agataaatgg
     3901 cgaccgtgat gtccttgatg gtttcagaca actcgaacag cgtttcccca ccctcgttgg
     3961 aaagcttgag aggaacctcc ataggttggg tttggaaacc ttcaacgacc tggttgatat
     4021 aacaatagcc attggccgga ttgagctgga catgcgctcc gcccgttttc acgcgctgat
     4081 tggccgtgtc gaactccaaa agccaagaaa atcggccaga accttcttga aaacactggg
     4141 gaagattcaa cgccacaccc ttggtgacca cgttgtcctg aagaaattgc ccagccaata
     4201 catccggttt gatgacttcg agttgcgaca tgcggaacga caacgtgctc gaacccgcgt
     4261 tgtcatgcca cgccatgcaa gcccgaccca caccatcgag accacagccc ggaccagaag
     4321 gccaacacgt gtttgtgctc ggaccgcccg caccgtcctt gtcttcttcc ccacaggccg
     4381 caagcataag catggcaagg gtggtactta ccgcaagcga cgtcatgatc gtaagcttcg
     4441 tgcgcattcg ttgaggcctc ctgttcgaac tgagcgatgc caagagcata gctggcctca
     4501 ctatggaacg cgacgtgttc ggtgatgatt ctcgaatgac gcaacgcgac ccgatgggat
     4561 gctgcaatgc gttgtgcatg cgaaatgcag tgttttcagc ccatgcgata gtctaccaac
     4621 atccaagcct tcgagaaggg acggcatcgg atgtgtcgtc gagccgaccc accgcttgaa
     4681 aacgcagcgc gtcgttttcc cacgggatgc ccactgctcc ttgcacgata gcgacccgat
     4741 ctggcgtgat cgaaaggtca accgtcaaat cgaccacaaa ctccgaaata ccatcggcat
     4801 cctgtccctg tatcggcact cggtgaacgg atgatacatg gctggctgag gcttcgcgca
     4861 cggcccccgt cgacgccgtc acgctcgtaa gcgcttcgaa tcgacccgca tcgcaaggca
     4921 tcgacgcatg ccacgatcga agaacgacgt cacaggcatc gctttccaac atcagccaaa
     4981 cgtgtggaaa actcgagcaa ggcgcgtgat cggaggatgt ctcgacaacc gtttgcgcca
     5041 ccaagatgaa cggattgccc aaggatacag attcccgttc gcctgccgga caggagggct
     5101 tcacgacggg agcggccgta acttctcgcc aagggtcggt gttatcgttt tcgacggggg
     5161 cgcaagccat cagccatacg gttcccaacc acagacaacc aagcttgttc atgaattcct
     5221 cagtggcgcg aaggcacacg gctatggcta aggcgggatt tggtcaagag tagatgtgat
     5281 gaaatgtcgt tatttcgagc atgttctcat tctgttcacg gaatgtgagc ggttgggcct
     5341 ttcgccacgc ggtagagtag gtaccaagtc gcggtaagct gtactagggt cccgcccggt
     5401 tcactgtgtt tcattcgatt atcgagcatg tttttgaccc agcctggaac gccgcccatc
     5461 gggtggtgat caatgtcggc atcgtggtga ttctgttcct ttccgggctt ggacttcccc
     5521 ttccagaaga tatccctctg tcagccgcag ggtttacgac cttcaaacag gcgggagatg
     5581 aattcgtgtg gtggcgctat ggcctgacgt tcgccatggt cgtcgtcccg attcttctgg
     5641 gagatatgtg tgcctggacg ctcgggcgaa aatatggttt gccgattcgc gatcgattca
     5701 agattttgcg tcgcgtgatc gatgacaagc gggtggcgcg cgtgcggtcc tggttcgaac
     5761 gatacggcgg tgccgcggtt tttttgggac gacagctcgc gggggtacgc ttcgtaacgt
     5821 ttttcatggc cggagtgatg cgtgtcaccc tgccaagatt cgtgttctgg gacttcatcg
     5881 gctgcgtcgt gagcgtcccc atatggctta ccctcggcac gctcgcggct cgctatggaa
     5941 gagagtggct cctcaaagct tcccataccg tgggacacac attccttttg atcgtccttg
     6001 gcgccatctt gattctcttt cttgtcacca aacttcgcgc cagacgggaa aattccaaca
     6061 cgtaatcgtc tacggatggt gtcgccgccc cttgacgccc cgttcgaagt gtttcaaacg
     6121 aatccttcga aacccgcgtt cgtgtaaggc cacatgaacc gacttacgcc atctgctcca
     6181 acaccatccg accccatcga cttgggaacc accgacgagc tgcctgccat gggcgattgg
     6241 gtggaagaca cgagcgtcga gccacgagat tccgcgcacg acaccgtccc ttccccgcct
     6301 cccgatggtg aatacgccag gatcaccttg ccgaccgtgc cttccgtcgg aaagctcctg
     6361 gactttctcg atgaggaggc taccgatccc ggttccgttg gctgagcctt ctcaacgccg
     6421 atcttcaggg tcgtacgata gtctcgaacc caaccatttt tcgacttccg tcacggacgt
     6481 tgattttcgt tttgcataat cctgcacctg gtcttttcca attcgcccca cgctgaagta
     6541 ggtggcggcc gggtgcgcca gatagatgcc gctcacgctc gccccaggcg acatcatgaa
     6601 actatcggtg agcgagacac caatttccga ggcttcgagc agcgaaaata gcttttcctt
     6661 ctccgtatgg tcggggcaag cagggtatcc aaaagcaggt cgaatgcctc gatagcgttc
     6721 cgcgatgagg tcttcgttcg tgaaattttc ccccacgcca tatccccaat cccttcgtgc
     6781 ttgcgcatgg agtttttccg caagcgcttc cgcaagtcga tccgtcaaag ccttggtcat
     6841 gatcgcgcgg taatcatcgt gctcacgctc ataccgttgc accaactcct cggctccgat
     6901 tcccgcggtg actgcgaagg ctcccacgta atcaggctgc tcactgccca ccttggtcaa
     6961 aaaatcagac agacaaagat aaggctcctc ggccctgctt ttctcccgct gctgacgaag
     7021 catcagaaac cgcaattttt cctcgcggcg tgtctcgtcc gtatacacca cgatgtcgtc
     7081 gccatccgcg tgcgccggcc aaaaaccata gaccgcgcga catcgcaccg agcgctgctc
     7141 atgcatttgc cgcagcatct ccttgccatg atccaataac tcacgagctg ccttcccata
     7201 ccgcgtgctg ttgagaatcc cgggaaaact ggccttcatc tcccacgcca aaaagaaaaa
     7261 tgtccaatcg atgaaaggct caatctgcgc aatcggtaca tcctcgatca cgcgtcgccc
     7321 gataaaatcc ggtcgcgcca ggtcttccgc gcgaaactcg atcacctttc gacgatcacg
     7381 agcctgctgc aacgacaaca tgggccgggt ggctttggcg ccataaatct cacgcaaacg
     7441 atcctgctcg ctgaacgtct cttccgtgaa ctttctgcgc tgcaccggat cgcgaagctg
     7501 cgataccacc cccactgccc tcgaggcatc gagcacatga gccaccacgc cctgatgggc
     7561 cggtgcaatc ttgaccgccg tgtgctgccg actcgtcgtg gctcctccga tgagcaaggg
     7621 aatctgcagc tcccgacgct gcatctcctt ggccacggtc accatctcgt ccaaacttgg
     7681 tgtgatcaaa ccgctcaaac cgatcatgtc cgccttatgg gtgatggccg cttccacgat
     7741 ccgattggcg ggcaccatga cgcccagatc cacgacttcg tagttgttgc agccaagcac
     7801 caccgcaacg atgtttttcc cgatgtcgtg gacatcgccc ttgaccgtgg cgagtacgag
     7861 tttggctttg gagcgcgatg cttgcgcatc atccttttcg gcttccaaaa atggttgcaa
     7921 atacgccacg ccttttttca tgacccgcgc acttttgacc acctgcggaa gaaacatctt
     7981 tccagcccca aacaagtccc caacccggtt catgccgtcc atcatcggac cctcgatcac
     8041 cttcaacgga cgaccatatt tcactcgcgc ctcttccacg tccgcttcga tatggtccac
     8101 gatgccaagc accagcgcgt gctcgatccg cttttccacc gatgcctctc gccagctcgt
     8161 gtcgatgctt cgcttggcgc cggttccctt gtaccgttcc gccaattcga tcatccgatc
     8221 ggtcgagtcc gcgcgacgat cgaacaacac gtcctcgaca cgacgtagca actcctccgg
     8281 gatatcctca tacaccgcga gttgccccgc gttgacaatc cccatgtcca ggcccgcgcg
     8341 aatggcatga tatagaaaag ccgcgtgaaa ggcttcccgc accggctcgt tgccgcgaaa
     8401 ggaaaaggat agattgctta tccctccact taccttggct ttgggtagct cggccttgat
     8461 gcgtcgaacc gcctcgatga actccatcgc atagcgattg tgctcctcga ttccggtggc
     8521 tacagcgaat acgttcggat cgaagatgat gtcctccggg ggaaatccaa cacgttcggt
     8581 gagaagtcga taggcacggc tgcatatctc cactttccga tccgccgtat ccgcctgacc
     8641 ttgttcatca aaagccatca ccaccacggc tgctccaaaa cgacggacct gccgcgctcg
     8701 ctcgacgaat gactcctccc cttctttcaa actcagcgaa tttacaatag cttttccctg
     8761 gtggcaccgt agccccgcct cgatcacgct ccacttcgag ctgtcgatca tcatgggaag
     8821 acgcgcgatc tcgggttcgc tcgctaccca cgtcagaaac gtcgacatgg ccgcttcggc
     8881 atccagcatg gcctcgtcca tgttgacgtc gaggatattg gccccaccgc gcgactgctc
     8941 cagcgccact tccaacgcgc gagaatactc cgctttcatg atcaatttgg cgaatcgacg
     9001 acttcccgtc acattcgtgc gctccccaat catgatgaag ttgctggtct cgtcgaccgt
     9061 caaggtttcc aaacccgcat accgtgtttt gccatcgtgc tcggggacaa cacgaggaga
     9121 tagccctttc atcgcttccc cgatcgcccg aatatgatca ggtgtggtgc cgcaacatcc
     9181 acccacgatg ttgagaagcc cgtcttcggc gaaa
//
LOCUS       DAOSOR010000182         9184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_7492_length_9184_cov_4.348450, whole genome shotgun sequence.
ACCESSION   DAOSOR010000182 DAOSOR010000000
VERSION     DAOSOR010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9184
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7492_length_9184_cov_4.348450"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="PK710_11385"
     CDS             complement(<1..898)
                     /locus_tag="PK710_11385"
                     /inference="COORDINATES: protein motif:HMM:NF013384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HOT10368.1"
                     /translation="MSRKQPISEPHKIKTVRRIAFPSIEERKRNLASAAFNVWRLTPS
                     QVTFDMCSAGTSALSQEQLAGQLIGDEAYAGARNFESLQLAVRDVFGHSYVCPTHNNL
                     GSVKLVVATMVPSGKTIPSNARTRADVLEPRGVQVPDIRDRDEKVFTGNVDLKKLEQL
                     IEKGNVALVGMQAFADGQHPFSLENLRAVHALCSRYGLRLVLDGSRVVENAWYIQRHE
                     VGQADLSIADIVKRIAKTASIFQIDGAQDAKCNTGGLLATDNPDDYEKVMNEVVVYEG
                     LHTYGGMAGRTMEVFARGLREMC"
     gene            complement(1276..2169)
                     /locus_tag="PK710_11390"
     CDS             complement(1276..2169)
                     /locus_tag="PK710_11390"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT10369.1"
                     /translation="MSWSRADVKVGFACNNRCLFCAQGHKREGCAHVPFQELVERMRQ
                     GRAASHELVLTGGEPSLHPDILALVREARQMGYQQVQLQTNGRRLAYASFVDALIQAG
                     VTEISPALHGPTADIHDALTRAPGSFRQTVAGIRVVRSRGLPIVSNSVVVRDNVEHLV
                     ALVGVLGKLGVGQAQLAFVHPVGTAYELFDRVVPRLSDVVEPIRACRKIAARYRMRLV
                     TEAIPLCFLRGMEELAVEDTIPNTTVIDLDGMPFDYSMWRPSEGKLHGPPCDACAAKG
                     RCEGPWREYPERFGWEEFRPL"
     gene            complement(2186..2929)
                     /locus_tag="PK710_11395"
     CDS             complement(2186..2929)
                     /locus_tag="PK710_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOT10370.1"
                     /translation="MVEHGEDGYDQPRSNMSNTDCMVEPARQDVEAVNDELARRHPID
                     DYYDRSIFPIRWIEKRRLAIIRSFVDERDGMRIAEVGSGGGHVLRMFRSSKLTAIDVS
                     NVFLDTARKNLRGYDVEFIKGEVDKLPVLPTGFDRVICTEVLEHTLDPQAILATIVRM
                     LAPGGRAVITVPHDPLIMKLKTMVRRSPAGWVLREKINWGGDHLHLHQWTPSSFRQLL
                     SRNYHVLEAAAAPSAVLPIRLCYLCAPKR"
     gene            3039..5531
                     /locus_tag="PK710_11400"
     CDS             3039..5531
                     /locus_tag="PK710_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10371.1"
                     /translation="MRFPALSPYTTLIIAAGLMMVLSTAACVSWRSRPPAEQQAAQAT
                     FGNVSTGRIAVHDDPGRPRFTSIVRQGDPAGAIAALVLTGGDGQASVALATIVAERLK
                     NAQGRPVEVHSDRLGYRISTLVQDPNEARAWVAALRNAFERPIQPDSPETKAAANALQ
                     SLRGSSPSRLELDAINACTGEIAIPASTTVLVPSTPDGARILEQWRASFHGAQRIAFA
                     TVGSSVLTTAVHEELNAGSPWPTVTPSEDPWPEADTVEAVFLTGSRTDTPVLTLAARV
                     SNTFAVTRLTETTFAASGPLAQRLAAATAWRLARSSATVRPRGACLALSVERDPALKD
                     VDLTLDAARVAAWLSHEMQGELQDAYADASVAGEIVLRTSNPQQAASIAAWWSHAARL
                     QAGPSRVAIALGLPPPKDIRDKSELGRTLTTALSSFQSSLPTFSASWQQPNIDTISRL
                     EEGQGEFWLLLANRCGTTAETGTDVGYSALAAIAAAHHHNGKDDVTLEPWIAQDGLGI
                     LAHASPKVGEHPSALARRVANAAASAMLGPPPPEYAIQEARHRLIAYLHTDGPQGEGF
                     GMLAKTLSPRQPAWLAPYGLYKTIVQADVQSITLRHAALASGPLRLAVLANSDAQQVP
                     SAARVTDRWIIHRSGSPRTCSPSPPPSSGPDATNIALHSDTSSQAWIGVAQPSPSPSD
                     RLMFAMLREILAGSEGMLPESFRSLDTSLRAEARTTGQRRMTALVLSFRGADEAVDRA
                     LQLAQMAMTRLRQGNISEQELRRTYTLVETNLLHQRLDPRVRIAQTWRQETSLPPMPS
                     IQEWKTWVARTVSDERIVTIRVRPHTSNSASN"
     gene            complement(5740..6339)
                     /locus_tag="PK710_11405"
     CDS             complement(5740..6339)
                     /locus_tag="PK710_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10372.1"
                     /translation="MTTTDSGPSASLDSLAQRFSPSMNAVTSPYGILCDLTFTFAVVA
                     AVGISTAAVFHYFPAIPGWVAIIPLAIPFLINAAAHLALCGARHRVVNWLMGVPFPVE
                     NVNAVLCGVGEQFDVTFEEAVPSRDALMQYLARASEDAYVLEIDESRRAMLARFGVVE
                     SKHNPHREAHRRFKRMQKVVSLALIPMHEEHRIERVLIV"
     gene            complement(6336..8141)
                     /locus_tag="PK710_11410"
     CDS             complement(6336..8141)
                     /locus_tag="PK710_11410"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT10373.1"
                     /translation="MAEDVFGIVGTLVGGAFNVEAVVAEGGFAVVYRAYHAAFHAHVA
                     LKCLKVPVVRRVDRREFLERFREEGEVMFRLSQSIPAVVRPLHVDAFETASGVFVPYM
                     ALEWLDGETLDVMIARRRADGLNPMSPKRVVRLLAPVARALQRAHRFPGKDGPVSIVH
                     RDLKPDNIAIATVNNEEVIKILDFGIAKVKSVASQVAGRQSQEAPAAVPFTPGYATPE
                     QWIPKRYGQTGPWTDVWGLALCAVEAMSGKPAIDGDHAAMMGTALDERRRPTPGSEGV
                     RVSDEVEAVFEKALAVDPRDRYHDVGRFWDDLEQALGLSSTRMLAGGLSSVLDPRSEG
                     PPPMREERIEAADHRLRKRISVSMDKSEEKRSPMSVPDLDPTPLSPGQPNTNLEVDLS
                     QEAKLAEIAPVPMPATSTAEQESVMDLAAPESSSSEGRAPASGALTDVQRIAEESSDH
                     HGMAEVRGEPSGIDLAVSPGEVRRVSSSGMRAVNPEQSKLALAVSPQDVRRTSNVAMR
                     AVRPAPPVPSANLGTRSDGYRPVSTLDSSKSKRLGIPIVLAVVGILVALAYYVNASMG
                     GGRWALGPVPVNWIAGLLTVAGTGMILVRLFWPEE"
     gene            complement(8260..8757)
                     /gene="purE"
                     /locus_tag="PK710_11415"
     CDS             complement(8260..8757)
                     /gene="purE"
                     /locus_tag="PK710_11415"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOT10374.1"
                     /translation="MRVLVVMGSDSDLAQMTPAWVVLQSLGIGYEVKVASAHRSPERL
                     VQIAQRAREDGFGVILAGAGGAAHLAGVMAAHTTLPVVAVPIAVGTLGGVDALLSAVQ
                     MPGGVPIASVGIGGARNAGLFAAAILSASDPELTQRLQRFRQEQSESVLAADQRVQAQ
                     LKGNT"
     gene            8877..>9184
                     /gene="rnpB"
                     /locus_tag="PK710_11420"
     ncRNA           8877..>9184
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK710_11420"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         2131 a         3026 c         2243 g         1784 t
ORIGIN      
        1 cacacatctc acgaagacct cgtgcaaaca cctccatcgt ccgccccgcc atccctccat
       61 aggtatgaag tccttcgtac acgacgactt cattcatcac cttttcataa tcatcggggt
      121 tgtcggtagc gagcaaccca ccggtattgc atttcgcatc ttgcgcccca tcgatctgaa
      181 aaatgctcgc cgtcttcgcg atccgcttga cgatatccgc aatactcaga tccgcctgtc
      241 ccacctcatg acgctggata taccatgcgt tttccaccac gcgagaaccg tcgagcacaa
      301 gccgaagtcc ataacgactg caaagcgcat gcaccgcgcg caggttctcc agactgaaag
      361 gatgctggcc atcggcaaag gcctgcattc ccaccaaggc cacattgccc ttttcgatca
      421 gttgctcgag tttcttgaga tcgacattgc ccgtaaacac cttctcgtcg cgatctcgaa
      481 tgtccggaac ctgaactccc cgaggctcga gtacatccgc ccgcgttcgc gcattgcttg
      541 gaatcgtttt tcccgacggc accatggtgg caaccaccaa cttcacactg ccaagattgt
      601 tgtgcgtcgg gcatacgtac gagtgcccaa aaacatctcg aaccgccaat tgcaacgact
      661 cgaagttcct cgctcccgca taagcctcat cgccaatgag ttgccccgcg agttgttcct
      721 ggctcaacgc actcgtcccc gccgaacaca tgtcgaacgt aacctgtgag ggcgtcagtc
      781 gccatacgtt gaacgcggcg gaggccagat tcctttttcg ctcctcgatc gacggaaacg
      841 caattctccg aaccgtcttg attttgtgcg gctcacttat tggctgttta cgactcatac
      901 gggacaccct ctccgttgga agacagcgcc actcgtggat tgctccgatc gaatcaatcc
      961 tccgtgacac accacgacca taggaaagaa tcgcggtgct gtcacgtcgc aagaaaccat
     1021 caatcgaggc taaaaatcgt gatattcaca ggcttgtccc tccttccatg gtagtcactt
     1081 cgcacctatc cccatggcag tgaccgaaag gcttggtcat aaggttgaaa cgaacctgaa
     1141 ggttttctcc actcgaaaac tgctaaaatc caagagcacg aaacaatcgg acgaacatca
     1201 ttccgtgatt gtgaaaactt gccacgactc ttgaacggca tcatccccta tcggccatgg
     1261 catcgaggaa cccactcaca aagggcgaaa ctcctcccag ccgaatcgtt ccggatactc
     1321 ccgccaaggc ccttcgcaac ggcctttcgc agcgcacgca tcacacgggg gaccgtgcag
     1381 tttcccttcc gatggacgcc acatactgta atcaaaaggc ataccatcaa gatcgatcac
     1441 ggtcgtattg gggatcgtgt cttcgaccgc caattcttcc attccccgca aaaaacacaa
     1501 aggaatcgcc tccgtcacca atcgcatccg gtatcgagct gcgatttttc gacaagctcg
     1561 aatgggttcc accacgtcgg acaagcgcgg gacgacacga tcgaatagct cgtacgccgt
     1621 gcccacggga tgcacaaatg caagctgggc ttgtccgact ccaagctttc ccaacacccc
     1681 caccaacgcc accaaatgct cgacgttgtc ccgaaccacg acactgttgg atacgatggg
     1741 cagcccccga cttcgtacga cacgaatccc cgcaaccgtt tgccgaaaac tacccggcgc
     1801 acgcgtcagc gcatcatgaa tatccgctgt cggaccatga agcgcaggag aaatctccgt
     1861 caccccggct tgtatcaaag catccacaaa cgaggcataa gccagccttc gtccgttggt
     1921 ctgaagctga acttgttggt atcccatctg cctggcttcc cgaaccaacg caaggatatc
     1981 cggatgcaag ctcggctccc ctcccgtcaa tacaagttca tgactcgccg cgcgcccctg
     2041 tcgcatgcgt tccacgagtt cctggaacgg aacgtgcgca cacccttctc gtttatgccc
     2101 ctgggcgcag aataagcacc gattgttaca agcaaaacca accttgacgt cggctcgaga
     2161 ccacgacatc acgcgcctct cattctcatc gtttgggcgc acatagataa cataggcgaa
     2221 tgggcaatac ggcagatggc gcggcggcag cctcgagcac gtgatagtta cgggaaagca
     2281 gttgtcgaaa actcgatgga gtccattggt gcaaatgcaa gtggtcaccg ccccagttga
     2341 ttttctctcg aagaacccat cccgctggag atcgccgcac catcgttttc aatttcatga
     2401 tgagtggatc atgcggaacc gtgatcaccg ctcgcccccc cggagcaagc atccgtacga
     2461 tcgtggctaa aattgcctga ggatccagcg tgtgctcgag cacttccgtg catatcacac
     2521 gatcgaatcc cgtgggaaga accggaagct tgtcgacttc gcccttgatg aactcgacat
     2581 cataaccgcg aaggtttttt ctcgccgtgt ccaaaaatac attcgataca tcgatggccg
     2641 tcagcttcga gcttcgaaac atgcgcagca catgcccacc cccagagccc acctccgcaa
     2701 tgcgcattcc atcacgctca tctacgaaag aacgaatgat cgccaatcga cgcttctcga
     2761 tccatcgtat aggaaaaatc gatcggtcgt agtaatcatc aatcgggtga cgccgcgcca
     2821 gttcatcgtt gacagcttcc acgtcctgcc gagcgggctc aaccatacaa tcggtattcg
     2881 acatattcga ccgcggctga tcatagccgt cttccccatg ttcaaccatg aaaaccgcct
     2941 gaaccacctg tccaacggcg accccgttcc tccccgcaat gcaggaaaaa ccttgtcata
     3001 acgccagttc gtaccgtagg acctttccat cgctcaccat gaggtttcct gccctttctc
     3061 cgtacacgac actcatcatc gcagccggac tcatgatggt gctgtccacc gccgcatgcg
     3121 tctcgtggcg atcccgccct cccgcagaac aacaagccgc ccaagccacc ttcggcaatg
     3181 tcagcaccgg acgcatcgcc gttcacgacg atccaggcag accgagattt acgtcgatcg
     3241 ttcgacaagg cgatcccgct ggagccatcg cagctctcgt gcttaccggc ggcgatggtc
     3301 aagcctccgt agccctcgcc accatcgtgg ccgagcgctt gaaaaacgcg caaggacgtc
     3361 ccgtcgaagt ccactccgat cgtctcggct accgaatcag cacactcgtg caagacccaa
     3421 acgaagcacg ggcatgggtc gccgcactcc gcaatgcctt cgaacgcccc atccaacccg
     3481 atagccccga aaccaaagcg gctgccaacg cgttgcaatc acttcgaggc tcatcgccat
     3541 ccagactcga actcgatgcc atcaacgcat gcacgggcga aatcgccatt cccgcctcca
     3601 caaccgtgct cgttccttcc acccccgatg gtgcgcgaat cctcgagcag tggcgcgcct
     3661 cctttcatgg cgcacaacgc atcgccttcg cgacggttgg ctcctccgtt cttaccacag
     3721 cagttcacga ggaattgaac gctggttccc cgtggccaac agtcacacct tcggaagatc
     3781 cttggccgga agccgacacg gtggaagctg tttttctcac aggatctcgc acagatacgc
     3841 cggtactgac cctcgcagcc cgcgtgtcga acaccttcgc cgtcacccgg ctcacggaaa
     3901 ccaccttcgc ggcttcaggc cccctcgccc aacgactcgc tgccgccaca gcctggcgac
     3961 tggctcgctc gagcgccacc gtccgccccc gaggagcctg cctcgctctg tccgtcgaac
     4021 gggatcccgc tctaaaagac gtggatttga cactggacgc ggctcgagtt gccgcatggc
     4081 tttctcacga gatgcaaggt gaattgcaag atgcctacgc ggacgcttcg gtcgcgggcg
     4141 aaatcgtgtt gcgcacctcc aacccccaac aagcggcttc catcgctgct tggtggtccc
     4201 acgccgctcg cctacaagct ggcccgtcac gcgtagccat cgctcttgga ctccctccgc
     4261 ccaaggatat ccgcgacaaa tccgaactag gccgcacgct cactacggcc ctttccagtt
     4321 tccaatcttc gttgccaacc ttctccgcct cttggcaaca gcccaatatc gatacgatat
     4381 cccgactgga agaaggacaa ggcgagttct ggctgcttct cgcaaaccgt tgcggaacga
     4441 ccgcagagac aggaaccgat gtcggctatt cggccctcgc ggcgatcgct gcagcccatc
     4501 accacaacgg taaggacgac gttaccctcg aaccatggat cgcccaagat ggcctcggca
     4561 tcctcgccca cgcttcacca aaagttgggg aacacccaag cgcgctggca cgacgcgttg
     4621 cgaatgccgc agcctcagcc atgcttggac cacccccccc ggaatatgcc attcaagaag
     4681 ctcggcatcg cctgatcgct tatcttcaca ccgacggacc gcaaggcgaa ggatttggca
     4741 tgctcgccaa aaccctgtca ccacgacaac ccgcttggct cgcaccctat ggattataca
     4801 aaaccatcgt tcaagccgat gtccaaagca taaccctgcg acatgccgcc ctggcttccg
     4861 gtcccctgcg attggccgtc ctggccaatt ccgacgcaca acaagtgcca tcggctgctc
     4921 gggtcaccga tcgatggatc atccaccgtt ccgggtcacc acgcacttgt tctccgagtc
     4981 caccaccgtc atccggccca gatgccacca atatcgccct gcactccgat acttcttcgc
     5041 aagcctggat cggagtcgcc cagccctccc cctccccatc ggaccgtctg atgttcgcca
     5101 tgctccgtga gatacttgcc ggttcggagg ggatgctgcc cgagtcgttt cgttccctcg
     5161 atacctcatt gcgcgcggag gcaaggacaa ccggacagcg tcgcatgaca gctttggtat
     5221 tgtcgtttcg cggagccgat gaagccgtcg atcgggctct gcaactagcc caaatggcaa
     5281 tgacccgact tcgacaagga aatatttcgg aacaggagct tcgacgaacc tacacgttgg
     5341 tcgaaaccaa tttgttacac caacgcttgg atccacgagt ccgcattgcc caaacttggc
     5401 gtcaagaaac gagcttgccc cccatgccaa gcatccaaga gtggaaaaca tgggtggcgc
     5461 gtacagtttc cgacgaacga atcgtcacca tacgcgtacg accccacacc agcaatagcg
     5521 catcgaatta gcctccgatc ctatcatgag cattcgctcg atgccatgac gctcccaacc
     5581 aagttgattt atcctccgtt tcgattcacc acgctctcgc actccaaata caacactgcc
     5641 ctcaacgcta gatcgttgtg tcgaatgata tacggcgaac gaatcatctc cgtgcgcgtt
     5701 cgtcctcaac cagccatggc gcatcgaacg atctacccat cacacaatga gcactcgctc
     5761 gatgcgatgc tcctcatgca tgggtatcag tgccaacgaa acgactttct gcattcgctt
     5821 gaatcgacga tgcgcttcgc gatgcggatt gtgcttggac tcgacaaccc cgaatcgagc
     5881 gagcatcgca cggcgtgact catcgatctc gagaacataa gcgtcttccg atgctcgcgc
     5941 caagtactgc atcagcgcgt cgcgggaagg caccgcctct tcgaaagtca cgtcgaattg
     6001 ctccccaact ccacataaca cggcgttcac attctcgacg ggaaacggaa cgcccatgag
     6061 ccaattgacc actctatgtc gcgcaccaca aagagccaga tgggccgcag cgttgatgag
     6121 aaaaggaatt gccaagggaa tgatggcgac ccatcctggg atcgcaggaa agtaatgaaa
     6181 cacagccgcg gtcgaaatcc caaccgcagc aacgaccgcg aaggtgaacg ttagatcaca
     6241 caggatccca taaggagacg tgaccgcatt catgctcgga gagaatcgct gggcgagact
     6301 gtcgagcgaa gccgacggac cagaatcggt ggttgtcatt cttcgggcca aaacagtcga
     6361 acgagaatca ttcccgtacc cgccacggta agcaatcccg caatccagtt gacaggcacc
     6421 ggtcccaacg cccagcgtcc ccctcccatg ctggcgttga cgtagtaagc aagagccaca
     6481 agaatcccga cgacagccag gacgataggt atgccaagcc tcttcgattt ggagctgtcc
     6541 aacgtagaaa cgggacgata cccatcactt ctcgttccca aattggcgga aggcacaggc
     6601 ggcgcaggac gaactgctcg catagccacg ttcgaggttc ttcgaacatc ctgcggagag
     6661 accgcaagag cgagcttgct ttgctcagga ttgaccgcac gcatccccga ggaagacact
     6721 cgtctcactt caccgggcga gaccgcaaga tcgatcccgg aaggttctcc gcgcacctcc
     6781 gccatgccat ggtggtcgga gctttcctcg gcgatccgtt gaacatcggt aagagcgcca
     6841 ctggccggag cacgtccttc ggaagaagaa gattcgggag cagccaagtc catgaccgac
     6901 tcctgttccg ccgtggatgt cgccggcatg ggaacaggtg caatctcagc aagctttgct
     6961 tcctgcgaaa ggtcgacctc caagttcgtg ttcggttgcc cgggtgaaag aggcgtggga
     7021 tcgaggtcgg gaacactcat gggagaacgt ttctcttccg acttatccat cgaaacagaa
     7081 attcgtttcc gaagccgatg atccgccgct tcgattcgct cttctcgcat tgggggcggc
     7141 ccttcgcttc gcggatcgag caccgaagac aatcccccag ccaacatacg cgtcgatgac
     7201 aaacccaagg cttgctcgag atcatcccag aatcgaccca catcgtgata ccgatcccgg
     7261 ggatcgacag ccaaagcttt ctcgaacacc gcctccactt cgtccgatac acgaacccct
     7321 tccgatccag gcgtaggacg tcgacgctca tccagcgcgg ttcccatcat cgccgcatgg
     7381 tccccatcga tggctggctt gcccgacatc gcctccacag cgcataacgc caacccccaa
     7441 acgtccgtcc aaggccccgt ctgtccataa cgtttgggta tccactgctc tggtgttgcg
     7501 tagccgggcg tgaaaggaac cgccgcgggc gcctcttgag actgacgacc tgcaacctga
     7561 ctcgccacgc ttttgacttt ggcaattccg aagtcgagga tttttatgac ttcttcattg
     7621 ttcaccgtgg caatggctat gttgtccggc ttcaagtcgc gatgcacgat ggatacggga
     7681 ccatctttgc cagggaaccg atgcgcccgt tgcaaagccc gcgcaaccgg cgcaagcaat
     7741 cgcactaccc gtttcggact catcggattg aggccatcgg cccgacgccg tgcaatcatc
     7801 acatccaacg tttcaccatc gagccattcg agcgccatgt agggaacgaa gacaccggag
     7861 gcggtttcga aagcatcgac atgcaatggg cgcacgaccg cgggaatgct ctgcgagagg
     7921 cgaaacatca cttcgccttc ctcccggaat cgttcaagaa actctcgtcg gtccacccgt
     7981 cgaaccacgg gtactttcaa gcatttgagg gcaacatgag catggaaggc ggcgtgatac
     8041 gcacggtaca cgaccgcgaa gccgccttca gcgaccactg cttcgacgtt gaacgccccg
     8101 ccgacgagcg tgccgacgat tccgaaaacg tcttccgcca tggggacacc tagcctacaa
     8161 gagcccgcgt gcggaaagaa gggccctggc gctttttgca cgggaataat ttcgaaaaat
     8221 cggaaaccct gtgcattggc aggcaaaaac gcgcttgatt cacgtgttcc cctttagttg
     8281 tgcctgtact cgttggtcgg ctgcaagcac gctttccgac tgctcttggc ggaaccgctg
     8341 aagcctttgg gttagttcgg gatcggaagc actcaaaatc gcggcagcaa acaagcccgc
     8401 gtttctagct cccccaatgc ccaccgacgc aattggcact ccccctggca tctgtaccgc
     8461 cgaaagcaaa gcatccacac cacctagggt tcccaccgcg atgggcacgg cgacgacagg
     8521 aagggttgtg tgggctgcca tgacacccgc tagatgcgcg gctcctccag caccggccaa
     8581 aatgacacca aaaccatcct ctctcgctcg ctgcgcaatc tgtacgagcc gctccggaga
     8641 acgatgcgcc gaggcaacct tgacttcata cccaatacca aggctctgca acacaaccca
     8701 cgctggagtc atttgcgcaa ggtccgaatc agaacccatc accaccaata ctcgcatcaa
     8761 actctccacc gctctgaatc caaagccctc attccaatac cagcatccac cgttgcgcga
     8821 acccaaggag aaagcaagat cccatccgcc cgaaatcctg ctatcatgcc cccgtctcag
     8881 aagaataggt gaccgtcggc cgaaaggcgg gaggaaagtc cgagctccac agggcagggt
     8941 gccgggtaac ccccggtgag ggcaacctca aggaaagtgc cacagaaaac aaaccgcgcc
     9001 cccggttcgt ccgggacgta agggtgaaaa ggtggggtaa aagcccaccg cgcattcggt
     9061 aacgagtgcg gcacggcaaa ccccacccgg agcaaggccg cataaaggga caatatagca
     9121 tcaccatgct gtgagaaggt cgccctccta acgtccccgg gttggccgct atcgatgcca
     9181 tggg
//
LOCUS       DAOSOR010000183         9172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_7506_length_9172_cov_4.017769, whole genome shotgun sequence.
ACCESSION   DAOSOR010000183 DAOSOR010000000
VERSION     DAOSOR010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9172
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7506_length_9172_cov_4.017769"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2876
                     /locus_tag="PK710_11425"
     CDS             <1..2876
                     /locus_tag="PK710_11425"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOT10375.1"
                     /translation="VALIDEFQDTDAMQWKIFRTLFSGEDQRLYLIGDPKQAIYGFRG
                     ANVHVYLKAKKEARGRVSTIVTNHRSDQRFVEAINHLMNRRGLFGQEGIEYQAVQAAI
                     RPQSPVDRLIPPDDWFSASNAPFQLRFLDRRLEGVAEATDADRRMPADLLYKNVPKRV
                     AEDIVLLLESGTRIFVERQALTGEDKTRCVEPSDIAVLTRTGKEANAIQQALGTAGVP
                     AVLMGADSVFASVEAFELLLWLEALDKAGHERAARAAATTHLFGRNAGLLAGIEAERP
                     EALEAWEAWMERLHRWRKRFENHGLLSTLRYALQDDLVAVDGEILQDATARLLMRPDG
                     QRRLTNVWHVAELMHGVEVTERRSLTGLIDWLKRERMNPSKETDAAELRLDRDDKAVR
                     ISTMHKAKGLQFPIVFVPYLWTGRLPKDTEPAVIVPETGDHAKRVCDLRDAADRQDSY
                     ELAVMESRKEAIRLLYVALTRARHRCVVYLGHINHLENSALAPVFHGHHAEGASIERG
                     VERAARENHSVLWKDLQKLASKSGSALEGNAPTISLTFCTPPEARIWKGQPGESGDLT
                     ARDFLRHGLDGSFRRLSYSALTYGKSVTYVDTDKDREGVDADEQTDDGDEAMVERQEQ
                     ENSEATDDQENDRSVSDPSSRWVPTIEVSEDSPNVPLAVMWGGADVGTFFHEVFEKAD
                     FRWAHPSQGADGGRTRLWELVRALLGVHGFDTQELTEPITAGLMDVLQTPLRSVLGDT
                     RLCDIAKEDRLDELSFDFPMAGGDRFRRGGPTKRVLSEAFVSALSLRLHEDNPPLDDD
                     APIRPAYLRGLGPFSKMVGYMTGSIDMVFRYPTEQGVRWFIVDYKSNRLDPQRQKRWP
                     IEHFGPEGMRYEMEQHHYYIQYHLYMLALHRYLRFRLGEAYAYERDVGGVYYLFFRGM
                     LGPATPVGRDTSHGCFFDKPPFRVIDALDRLFHDPCTFDGGQS"
     gene            2873..4921
                     /gene="recD"
                     /locus_tag="PK710_11430"
     CDS             2873..4921
                     /gene="recD"
                     /locus_tag="PK710_11430"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HOT10376.1"
                     /translation="MSLSLAPLLVGGSEGPFEQRLRPFWQRGVFASVDVHVVDHLGAL
                     VGESDPDVLLALALAVRAPRHGHICIDLAQMDTFAWQPESPREESALEPLPWPDKNAW
                     IAKVAASPLVRGAGQDRVTPFVLDGTILYSDRYFTYQSRLAQALRARAMQTQPIRDSV
                     GLRRGLEALFRSGAGEAFDRQRLGAAMALLGGLTVISGGPGTGKTHTIRAVLALAWAQ
                     WAVDHDPQRDGPGPLVALAAPTGKAAARMREALGAGLEAFVQQTGSLLPKRRNPEQLQ
                     VFMESLQPCTIHRLLGYNPERPTRFRHDAQWPLAHEMVIIDESSMVDLALMTKLVDAV
                     SPQARLVLLGDRYQLASVEAGTVLADICGPSTVKRMGISEDRVGELATYSGIDVAEQP
                     FLERKGERGIQDAIVLFEKNWRFDPSSGMGSFAKACLDELVEPSQVADILMKFCDSEV
                     AEQASSASKAIPETIRKVIVEGYRPYLERLLAGPKGNESLAAFHAAVLELFGSFRILC
                     AHRDGRWGVTGMNREVIRLLEESSEQTPLQRFDPSRVYWVGRPVMILRNDAIVQRFNG
                     DVGIVVKDEKGKTTVVFPEVGGVSYIAPSRLPEHQTVFAMTIHKSQGSEFDHCLVVLP
                     DRDSRILTKELVYTAVTRAKHTITMMAQRQVLVEALGRTIRRASGLEAKLWETGKGSA
                     "
     gene            5417..6781
                     /locus_tag="PK710_11435"
     CDS             5417..6781
                     /locus_tag="PK710_11435"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOT10377.1"
                     /translation="MLRRTLFALSLLTLFSSQACSQGDEDSSSQGTVTPDHAPKTARS
                     ELQRDPAPQVSDEAKSALVQGHNAFAFDMYDLIRQEEGANENMFFSPTSMTLALSMVY
                     AGARGTSATEMATALHFTLPAEQLFPAFNWLDQALGQRADKAYQDAQSDWENGKRGDP
                     PSADSFRLHIVNSIWGEQTMTFESPFLDTLAVHYGAGVYLSDFIHKPNDERLRINDWV
                     ASETRDLIKDLLPEATITDETRTVLVNAIHLKFPWQEKFSPSEDVNFSLLDGSLAAVP
                     AIEHSDTWPYYEKDGLQAVRVALEGSEIELVVMAPPKGQLGSFETGLTQESLATLFEG
                     MESQAVTLQIPILRFTTPSISLRSKLETLGMPSPFDPSRADFSAITKDESLYLSDVIH
                     KAMIGIDKNGIEAAAATAAIANELSSVADPVPFTVDRPFFLALVDAPTKTILFTGHIV
                     DPRP"
     gene            7034..>9172
                     /locus_tag="PK710_11440"
     CDS             7034..>9172
                     /locus_tag="PK710_11440"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT10378.1"
                     /translation="MNPISEHPHPESIPLAPWTAPPLRPFRSALEHLEALIARMANLA
                     AAHLARIPAERYYREKTRGEEILEAVSFATGTPPPFTEQRWAIADAQLEWIRAAEAVT
                     TIDLPFRRLAAAFDLSDIEQDILLLVAAPKIDPRYEEYFQRIDREIVDPTIRTAIAVL
                     GRSFDACVTMRKLFSRDNKLIKNSLLLAEVRGNGEGDFLHVALEVPRRIVGEVLGESH
                     VAEELVALSRLRTSNVQLDQVVLPREVKETVVSLVRNHEEFVQRRQAWGLDDVIPYGR
                     ALIMLFSGPPGTGKTMLAHAVASTVNKRLFCIDLPKLVEERASVESNLDAIFREARLL
                     DAVLFFDECEQIFLSRSLGNDAIPVLLTRLEQYDGVAILATNREETLDEALARRVVAK
                     IDFGKPTASAREQIWRKHLPEALPLADDVDLPKLAERFDLTGGYIKNAVLTAVLRCVS
                     QGSEQVRMEDLESGARLQVRVTPDIFQKLQRPDVSLDDVVLPAPMQDRIARFVQSARA
                     RTTVLQDWGLGRNLGNIGGMTALFSGPSGTGKSMAAEAIAAALDRLLIRCPLSSVISK
                     YVGETSRNIASLFQVARENQAVLVFDEADALLARRTRVQTSNDRFANAETGALLSEIE
                     RYEGVVVLTTNLREALDSALERRLQLRLTFPFPTAPMRTKIWRRLLGTETPLGADVDV
                     RKLGRAYELSGGSIRNAVLAAALEAATAPLG"
BASE COUNT         1866 a         2450 c         2815 g         2041 t
ORIGIN      
        1 aggtggcttt gatcgatgaa ttccaagata cggatgcgat gcaatggaag atttttcgga
       61 ccttgttcag cggcgaggat cagcgtctat acttgattgg ggatccgaaa caggccattt
      121 acggatttcg cggcgcgaat gttcatgtct atctgaaagc gaaaaaagaa gcccggggac
      181 gggtatccac gattgtgacg aaccaccggt cggaccagcg ttttgtggag gcaatcaatc
      241 atttgatgaa tcggcgaggg ctgtttgggc aggaggggat cgagtaccag gcggtgcagg
      301 cggcaataag accccagtcc cccgtggatc ggctcattcc cccggacgac tggttttccg
      361 cgtcgaacgc gccttttcag cttcgtttcc tggatcggcg tctggaagga gtcgcagagg
      421 ccacggatgc ggaccgccgc atgcccgcgg atttgcttta taagaacgtg cccaaacgtg
      481 ttgccgagga tatcgtgctc ctgctcgaat ccggtacacg tatttttgtc gaacgacaag
      541 ctctgacggg agaggacaag acgcgctgtg tcgagcccag cgacatcgct gtcctcacgc
      601 gcacgggcaa ggaggcgaat gccatccaac aggctcttgg cacggcgggc gtccccgcgg
      661 tgctcatggg tgccgatagc gtttttgctt ccgtggaagc tttcgaacta ctgctttggc
      721 tcgaggctct ggacaaggcg ggccatgagc gcgcggcgcg agcggctgcc accacccatc
      781 tttttgggcg caatgccgga ttgctggccg gtatcgaggc cgaacgaccc gaggccctcg
      841 aagcctggga agcctggatg gaacgtctcc accgttggag aaaacgcttt gaaaaccatg
      901 ggttactatc caccctgcga tatgccctgc aagacgatct cgtcgctgtc gatggggaaa
      961 tcctgcagga tgccaccgca cgattgctga tgcgtcccga tggccaacga cggctcacca
     1021 acgtatggca tgtagcggag ttgatgcatg gggtggaggt gacagagcga cggagcctta
     1081 cggggctgat tgattggttg aaacgagagc gtatgaatcc gagcaaggaa accgatgccg
     1141 cggagttgcg tctcgaccga gacgacaaag ccgtgcggat ttctacgatg cacaaggcca
     1201 aaggccttca attcccgatt gttttcgttc cctatctttg gaccggacgt cttcccaagg
     1261 acacggagcc cgctgttatc gtgccggaaa caggcgatca tgccaaacgc gtttgtgatc
     1321 ttcgggatgc ggcggataga caggatagtt acgagttggc ggtgatggaa tcgcgaaaag
     1381 aggccattcg attgctgtat gtggccttga cgcgggcgcg tcatcgttgt gtcgtctacc
     1441 tggggcatat caatcacttg gaaaatagtg ccctggcgcc ggtttttcac gggcatcacg
     1501 cggaaggcgc gagcatcgag cggggggtag aaagggctgc acgcgagaat cattctgtcc
     1561 tttggaagga ccttcagaaa ttggccagca agtcgggctc ggcactcgaa ggaaacgcgc
     1621 cgacgatctc gctgacgttc tgtacgcctc cggaagcaag gatctggaag gggcaacctg
     1681 gggagtcggg ggatcttacg gcccgagatt ttctgcgtca tggtctggat ggaagtttcc
     1741 gacgactgag ctattccgcg ttaacgtatg gaaaatccgt tacttacgta gataccgaca
     1801 aggaccggga aggtgtggat gccgatgagc agaccgacga tggcgacgag gccatggtcg
     1861 aaaggcaaga gcaggagaat tccgaggcga cggatgatca agagaatgat cgttccgttt
     1921 cggatccgtc gtcacgctgg gtgccgacca tcgaggtgag cgaagattcg cccaatgtgc
     1981 cgttggcggt gatgtggggg ggagccgatg tgggaacgtt ctttcacgag gtcttcgaaa
     2041 aggcggattt tcgttgggcg catccgagcc agggggcgga tggggggcgg acccggctgt
     2101 gggaacttgt ccgggcgttg ttaggcgttc atggattcga tacgcaggaa ctcacggaac
     2161 cgattaccgc ggggttgatg gatgtattgc agacgccttt gcgctccgtg ctgggcgata
     2221 cgcgcctttg tgatattgcg aaagaggatc gcctcgatga gctgagtttc gatttcccga
     2281 tggcgggagg cgatcgtttt cgccggggag gtcccacgaa acgcgtattg tcggaagcct
     2341 tcgtctccgc tctttcgctt cgcttgcacg aagacaatcc gccccttgac gacgatgctc
     2401 cgattcgtcc cgcctattta cgagggctcg gcccgttttc caagatggtg ggctatatga
     2461 cgggcagcat cgatatggtg tttcggtatc cgacggagca aggggttcgg tggtttatcg
     2521 tggattacaa gtcgaaccgt ctggatccgc agcggcaaaa gcgttggccg atcgagcatt
     2581 tcggacccga ggggatgcgt tacgagatgg aacagcatca ctattatatc caatatcatc
     2641 tttatatgct ggccctgcat cgttatctgc gatttcgtct gggcgaggcg tatgcgtacg
     2701 agcgggacgt gggaggcgta tattacttat ttttccgagg gatgctgggg cctgccacac
     2761 cagtggggcg cgatacgagc catgggtgtt ttttcgacaa accccctttc cgtgtcatcg
     2821 atgccctgga taggctattt cacgatcctt gcacgttcga tgggggtcaa tcatgagtct
     2881 ttcccttgct cccttgcttg tgggtggttc cgagggtccg ttcgagcagc gactgcgccc
     2941 tttctggcag cggggtgttt ttgccagtgt ggacgttcat gtggtggatc atctgggggc
     3001 gctcgtgggc gaatcggatc cggacgtgct ccttgcgctt gctttggccg tccgtgcgcc
     3061 tcgtcatggg catatttgca tcgatcttgc ccaaatggat acgtttgcgt ggcagccgga
     3121 gagcccacga gaagaatcgg cgctcgaacc gttgccgtgg cccgacaaaa acgcgtggat
     3181 tgccaaggtt gccgcaagtc cgctcgtgcg cggcgcaggg caagaccgcg taacgccctt
     3241 cgtcctcgat ggcaccatcc tgtattccga ccgatatttt acgtatcaat cgagattggc
     3301 gcaggcgctt cgggcgaggg ctatgcaaac gcaacccatt cgggattcgg tggggctgcg
     3361 tcgtgggctg gaggcgcttt tccgttccgg ggctggcgag gctttcgacc gacagcgatt
     3421 gggggccgcc atggcgttgt tgggtggatt gacggtcatt tccggaggtc ccggcacggg
     3481 gaagacccat acgatccgcg cggtattggc gctggcctgg gcgcaatggg cggtggatca
     3541 tgatccgcag cgcgatgggc cggggccttt ggtggcgctg gcggcaccca cgggaaaggc
     3601 tgcggcaagg atgcgggagg cgcttggggc gggactcgag gctttcgtgc aacagaccgg
     3661 gagcctgttg ccgaaaagaa gaaatccgga acaacttcaa gtatttatgg agtcgctaca
     3721 gccttgcacg atccatcgat tgctggggta taaccctgaa cggcccacgc gttttcgcca
     3781 tgatgcgcag tggccgcttg cccatgagat ggtgatcatc gacgagtctt cgatggtgga
     3841 tctggcgcta atgaccaaac tcgtggatgc ggtatcgccg caggcgcggt tggtcctgct
     3901 cggtgaccgg tatcaactgg cttccgtgga agccgggacc gttctggctg atatttgtgg
     3961 gccttcgacg gtgaaacgga tggggatatc cgaggacagg gtaggggagc tggccacgta
     4021 ttcgggtatc gacgtggccg agcagccgtt cctcgagcga aagggggaaa gggggatcca
     4081 ggatgccatt gtgctattcg aaaaaaactg gcgtttcgac ccatcgagcg gcatgggttc
     4141 gtttgcgaaa gcatgtctcg acgaactggt ggagccgagt caagtagctg atatactgat
     4201 gaaattttgt gattcggagg tggctgagca agcgtcctcc gcgtccaaag cgattccgga
     4261 aacgatccgc aaagtcattg tcgaaggata tcgtccttat ctcgaaagat tgctggcggg
     4321 tccgaaaggc aacgaatccc tggcagcgtt tcacgccgcg gtactcgaat tatttgggtc
     4381 ttttcgcatc ctatgcgccc accgcgacgg acgctggggg gtgacgggga tgaaccgaga
     4441 ggtgatccgt cttctcgaag aatcgtcgga gcaaacaccg ctgcaacgat tcgatccctc
     4501 gcgtgtctat tgggtgggtc gtcctgtgat gattcttcgt aacgatgcga tcgtgcagcg
     4561 gttcaacggg gatgtaggca tcgtggtgaa agatgaaaaa ggcaaaacca cggtggtgtt
     4621 tccggaagta gggggtgtta gctatatcgc cccctcccgt ttgccggagc atcaaactgt
     4681 tttcgcgatg acgattcaca agagccaggg gtcagaattc gatcattgtc ttgttgtcct
     4741 tccggatcgg gactcgcgca tcctcacgaa agaattggtg tataccgcgg tcacgagagc
     4801 caagcatacg atcacgatga tggcgcaacg acaggtgttg gtcgaggccc tgggacgcac
     4861 gatccggcgg gcttcaggat tggaagcgaa gctttgggaa acgggtaagg gatccgccta
     4921 ggtcaagcat gggcaggaat gagcaggttg caattgacgc aacgtatgat gaaatagtct
     4981 atggtagagc atgatagtat ggcacaacgt tatgtgataa ctcctcatgg gcttgcgatg
     5041 gcgtttgtgt caacgtaact gcttgcgttt gacctgtctg ctatcaattc tatgacgcaa
     5101 cgtatggcga tgcttccgcc tgtcgaagtc ggcctgtcga cggggaagat tcggtaacga
     5161 cgtgtcgagg tgagatacgc gcgaaggcgg cgggagccgt cggcctggtc ggtcggagag
     5221 gtttcccttc cccgcttcag tcccttccgc ccctacgatg ccgaagcaac accttttgtc
     5281 tcgatctgtg tctatctgtg ccaagcccgg cgcgtcatgg catggtttgg atgatatttc
     5341 ccaggtcttt gcgtgatacg gcttggcccg ttcgttgcta gccagcttat cattctcacg
     5401 aaaagcgagg ttggatatgc tacgccgcac cctttttgcg ttgagccttt tgaccttgtt
     5461 ctcaagccaa gcctgctcgc agggggacga ggattcttcc tcgcagggga cggtgacccc
     5521 cgatcatgcc cccaaaactg ctcgctccga actgcagcgg gatccggcgc cccaagtaag
     5581 cgacgaagcg aaatccgcgc tcgtgcaagg ccataacgca ttcgccttcg acatgtacga
     5641 cctcatccgc caagaagaag gggcgaacga aaatatgttc ttttccccta cgagcatgac
     5701 gttggccctg tcgatggtgt acgccggagc acgagggacg agcgccaccg agatggcgac
     5761 ggcccttcat ttcacgctgc cggcggagca gcttttcccc gctttcaact ggctcgacca
     5821 ggcccttggg cagcgggcgg acaaggccta tcaagacgct caatcggatt gggaaaacgg
     5881 taagcgaggc gaccctccct ccgccgatag tttccggctt catatcgtca attcgatctg
     5941 gggggaacaa acgatgacct tcgaatcgcc ttttctcgat actctggcgg tgcattatgg
     6001 cgccggcgtt tatctttccg acttcatcca caagcccaac gacgaacgac ttcgtatcaa
     6061 cgactgggta gcctccgaaa cccgcgacct tatcaaggac ttgctgcccg aggctaccat
     6121 caccgatgaa acccgcaccg tgctcgtcaa tgccattcac ttgaaattcc cctggcaaga
     6181 aaagttctcc ccgtcggaag acgtgaactt ctcgctgctc gacggctctt tggcggcggt
     6241 gccggccatc gagcattccg atacctggcc gtattacgaa aaagacggcc tgcaggccgt
     6301 gcgtgtggcg ttggaaggct ccgagatcga attggtggtc atggctccgc ctaaagggca
     6361 gttgggaagc ttcgaaacag ggctcaccca agaaagcctc gcaacgttgt ttgaaggcat
     6421 ggaaagccag gccgtcaccc tgcaaatccc gatcctccga ttcacaaccc cctcgatcag
     6481 tctgcgttcc aagctcgaaa ccctgggaat gccctctccc ttcgacccga gccgtgccga
     6541 tttttcggcc atcaccaaag acgaatcgct ctatctcagc gatgtcatcc acaaagcgat
     6601 gatcggaatc gataaaaacg gcatcgaggc ggcggccgcc accgcggcga ttgccaatga
     6661 gctatcgtcc gtggctgacc ccgtaccctt taccgtggat agaccctttt tcctcgcttt
     6721 ggtggacgct cccacgaaaa cgatcttgtt caccggccat atcgtcgacc cacgccccta
     6781 aaagcagttc tcgacgatgc ctgagacgcc gctcgaacca tctcccccaa tgaccatcac
     6841 tcaacactat aacagttgtt aaacttccaa aggatggacg gaaccgatgg atcgatccat
     6901 tttaccgcaa aggtcatgaa atatcgtgtg ttaattcaat agcttacata gttgttgcca
     6961 gcgtcgatcc gagggccgac aaaaaatatt tgggttcagt tgattgttgc ctccccccaa
     7021 ttgtatatac ggtatgaatc ctatttccga acatccccac ccagaatcaa ttcccctggc
     7081 tccttggacg gccccgcccc tgcgcccctt ccgctccgcg ctcgaacatc tcgaagcgtt
     7141 gattgcgcgg atggcaaatc ttgcggcggc tcacctggca aggattcccg ccgagcgata
     7201 ctatcgcgag aaaacccgcg gcgaagagat tttggaagcg gtctcgtttg ccacgggaac
     7261 gccaccgccc tttaccgaac aacgttgggc gatcgccgat gcccagctcg agtggatccg
     7321 cgcggccgaa gccgtcacca cgatcgatct tccgtttcgc aggctggcgg ccgctttcga
     7381 cttatcggac atcgagcagg acattttgct gctcgtcgcc gcccccaaaa tcgatccccg
     7441 ctatgaagag tatttccagc gcatcgaccg ggaaatcgtc gatcccacca ttcgtaccgc
     7501 cattgccgtg ctcggacgct ccttcgatgc ctgcgtgacc atgcgaaaac tgttttcccg
     7561 ggacaataaa ttaataaaga attccctgct cttagccgaa gtccgcggaa acggggaagg
     7621 cgacttcttg catgtcgccc tcgaggtgcc acggcgtatc gtaggcgaag tgcttggcga
     7681 atcccatgtc gcggaggaac tcgtggcgct gtcgcgtctg cgcacgtcga acgttcagct
     7741 cgaccaggtc gttttgccca gggaggtcaa agaaacggtg gtgtcgttgg tgcgcaatca
     7801 cgaggaattc gtgcagcgac ggcaggcatg gggattggat gacgtcatac cttacggcag
     7861 ggcgcttatc atgctttttt ccggtccgcc gggaacgggc aagacgatgt tggcccatgc
     7921 ggtggcttct accgtgaaca aacggctgtt ctgtatcgac ctgccgaaac ttgtggagga
     7981 gcgggccagt gtggaatcga acctggacgc gatttttcgc gaggctcgcc tgctcgacgc
     8041 ggtgttgttt ttcgacgaat gtgagcagat cttcttgtct cgctctttgg gcaacgacgc
     8101 gattcccgtg cttctcaccc gtctggaaca atacgatggc gtggcgattt tggcgacgaa
     8161 tcgtgaggaa actctcgatg aggcgttggc tcgccgggtt gtcgccaaga tcgactttgg
     8221 gaaacctacg gcatcggcgc gggagcaaat ctggcgcaag cacctgcccg aggcgcttcc
     8281 tttggcggac gatgtggact tgccgaagct tgcggagcgt ttcgatttga cgggggggta
     8341 tatcaaaaat gccgtgttga cggcggtgct gcgatgcgtt tcccagggct cggagcaggt
     8401 tcgcatggag gatctcgaat cgggggctcg tttgcaagtg cgtgtaactc ctgatatttt
     8461 tcagaagctg cagcggccgg atgtcagtct cgacgacgtg gtgcttccgg ctccgatgca
     8521 ggatagaatc gctcgtttcg tgcagtcggc tcgggcccga actaccgtgc tgcaagactg
     8581 gggacttgga agaaaccttg gcaatatcgg cggaatgacc gcccttttca gcgggccttc
     8641 gggcacggga aaaagcatgg ctgccgaagc gatcgcggcg gcgctcgatc gtctgctcat
     8701 ccgttgtccc ctttcctctg tcatttcgaa atatgttggg gaaacctccc gcaatatcgc
     8761 ttcgcttttc caggtcgcac gggaaaacca agcggtactc gttttcgacg aggcggacgc
     8821 gctattggct cgtcgcacca gggtgcaaac ctccaacgat agattcgcca acgcggaaac
     8881 aggggcgctt ttgtctgaga tcgagcgcta tgagggagtt gtggtattga cgacgaatct
     8941 gcgggaagcg ttggattcgg cgttggaacg tcggttgcag cttcggttga cctttccctt
     9001 tccgacagca ccgatgagga cgaaaatatg gcgtcggctg ctcggaacgg aaacgccgct
     9061 cggggccgat gtcgatgtgc ggaagttggg gcgggcgtat gaattgtccg ggggaagcat
     9121 ccgcaatgcg gtattggcgg cggcgttgga agcggcgacc gcgcctttgg gc
//
LOCUS       DAOSOR010000184         9168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_7514_length_9168_cov_4.561615, whole genome shotgun sequence.
ACCESSION   DAOSOR010000184 DAOSOR010000000
VERSION     DAOSOR010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9168
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7514_length_9168_cov_4.561615"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="PK710_11445"
     CDS             complement(<1..1059)
                     /locus_tag="PK710_11445"
                     /inference="COORDINATES: protein motif:HMM:NF037180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10379.1"
                     /translation="MTHRIEVGYRPGFPDPRGMDTLSTIRSFLSIPIREVRCFDVYTI
                     EADLSEQEVIRACDHLADPVTQQGAVGRLAPAPFDFVVSVGYRPGVTDAVGKSALVLL
                     QDMAGRSLAPSSAVFTSRLTFLSGVNEQQAHRIATELLANSLIERFFILSQSDYLASP
                     IDLSIPVINSTHTPVCGAIDLSGSDQKLERLSKERILSLSLEEMKAIREAYEEPDFLR
                     ARTVVGLGAFPTDAELECLAQTWSEHCKHKIFNATIDYYEQGQESKTIESLFKTYIRE
                     PTERIRKQQETRRGESWLVSVFHDNAGVIAFNDDYHLVYKVETHNSPSALDPYGGAMT
                     GVVGVNRDTFGTGLGADLL"
     gene            1218..1598
                     /locus_tag="PK710_11450"
     CDS             1218..1598
                     /locus_tag="PK710_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10380.1"
                     /translation="MGHWNMPLLAVCVASLSSAATASPITSVAPHALDETSPPPAQCV
                     QASQSMHPSRDDTLIITLDNECSVAVNCSVSWVVRCKDGQEHSYSRSARLPSGEKRSF
                     EASASVCSEAGWRITPPRWECQSK"
     gene            complement(1603..2247)
                     /locus_tag="PK710_11455"
     CDS             complement(1603..2247)
                     /locus_tag="PK710_11455"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOT10381.1"
                     /translation="MARQIIDITLALRPAMTVYEGDPPFRCRPLTQKIEANLDTYNTS
                     ALSFGTHTGTHIDPPRHFDNQGQSVDRLDLSTLCGPARVFDLRGRGCRIDEAVVRGCD
                     GLGVERWLLRTDSEGWADRPFDRRYAHLTLDAACWLRSQGVRLIGIDSPSVEAFDSPT
                     MPVHRMLLTRHPAVLVLEGLDLTLVQPGDYELFCLPLKLEDGDGGPARAILVRN"
     gene            complement(2381..3190)
                     /locus_tag="PK710_11460"
     CDS             complement(2381..3190)
                     /locus_tag="PK710_11460"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOT10382.1"
                     /translation="MTMTPRQARHHALIAREAAHAAGQVALKGFRSHARIERKQGAEL
                     VTPLDFAAEDALRKSLQSADPTIPIVAEEGGGSASHDRYWCADPIDGTTNFAHGHPFW
                     AVSIALIEQGVPVVGAVVAPALDIEWIGWEGGLTLRNGEPCSVSDRSSIDDALLATGF
                     PYDRQTSPDNNIREFAYLQKRAVGIRRCGSAAIDLCLVSDGSYDGYWEKKLRPWDIAA
                     GVVMVRAAGGMATALDGQPDNVLDGHIVATNGHLHARLVELLRQSRQSTDW"
     gene            complement(3187..4647)
                     /locus_tag="PK710_11465"
     CDS             complement(3187..4647)
                     /locus_tag="PK710_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOT10383.1"
                     /translation="MHVHLIAVAGTGMGALAGLFKELGHDVSGSDVRFDPPMGPALEK
                     WGIRLMQGFDPAHLDPAPDLVVIGNVCRSNNPEARAAIDRGLPYTDMAHGLADLVLEA
                     KTPVVIAGTHGKTTTSSLAAWLLFDAGKDPGMLIGGLPKNFEHSFRIPSRKGPGLPLA
                     DHPMPTRRTPFVIEGDEYDTAFFEKTPKFWHYRPEIAVITSIEYDHIDIYPTFEAYLH
                     AFATFVERIPSSGLLVASAASQTVVDVVSQHARCEVIWYALQGENWHGCTPHYLGALA
                     GADATGQAFDLFIGGVASGRFALPMPGSYNVSNAIAAAAVAMQGFGVSVHQVRESLTR
                     FQGVRRRQDLVGTPNRINIYDDFAHHPTAVEETLRGLRARHPQGSLFAVFEPRSATAC
                     RALHQDAYAQAFTAASHVVIAPLGRPDIAQSERLDVPRIIDELQRQGQWARHPESIDE
                     IIDLLVREARPGDTIALLSNGAFGGIHNRLIQALSR"
     gene            4893..5495
                     /locus_tag="PK710_11470"
     CDS             4893..5495
                     /locus_tag="PK710_11470"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT10384.1"
                     /translation="MQTLAHYPKTVSIVDGQVVIRPMAQEDFDGVRRFFEQLPRQDRL
                     FLRHDVLRPEVFSRWFAELDYGKKLPLMALHGEQIVGHALLDGEQRSWSPHVAEIRVV
                     VADAFKKRAVGTVLARELFDQAMARGYEKAMASMMDSQKAARRMFERMGFRVEAELKD
                     HVKDLDGKKHNLVLMSCMLEDAWEKMSELLQDFSPWVGWH"
     gene            5699..7057
                     /locus_tag="PK710_11475"
     CDS             5699..7057
                     /locus_tag="PK710_11475"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOT10385.1"
                     /translation="MAASRPKRPSGKLATLVLALLAVGFASSVYLTNLYIEATEALKG
                     GTKLDSFCNLSQGVNCETVAASEYSAFLGIPISVWGLEYFGLAIAIVLASHYRLLSLK
                     RWDSLLFALSCTGVPVTIVLGYISVTIIQSVCIMCVTVYVVNTAMVLLLAGLNRSRLK
                     EFVREGPMELLALATHANHRVVTVFAVILGLSQFLWVPRLFARPTHGEDAASTLAAVS
                     NAKTGTWKELPASRNTLGPVNAPIRIEEFTDFQCPFCGRAHDVLLEVVTRYPGKIHLI
                     HRDFPLDHHCNPAIPEPFHPDACGAAYFARCAADQDLFWPYEALLFHNQRKLKKAAML
                     SFAQTVGLDTTALQTCVESKVTHQAVLADIDEGIKRGVAGTPAFFVNGEAIVGARPIE
                     FWEEKIQTLLGLAPPASASSATLSTSAAPPSSALPAMSAAPSASNPHPSPAASASVTP
                     SP"
     gene            complement(7228..7953)
                     /locus_tag="PK710_11480"
     CDS             complement(7228..7953)
                     /locus_tag="PK710_11480"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT10386.1"
                     /translation="MRTLFASFFIASFGFFGCSTSEDSTQRDAETTHDAATDMAPPDA
                     KPDSHADVTPDQNVSPDVSPDVPAESSEPSLFATIVTSKGTIRVKLADDASPITVENF
                     QRYADAQFFDGLIFHRVIAGFMIQGGGMEPGMKTRAPLFPPIVNEASASGLSNLRGTI
                     AMARTNQPDSATSQFFINTVDNTFLDPGQSSPAGYAVFGNVVEGMDIVDEIEAVATHT
                     VGIYEDVPVEDVVIESLRVTTEP"
     gene            complement(7987..>9168)
                     /locus_tag="PK710_11485"
     CDS             complement(7987..>9168)
                     /locus_tag="PK710_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT10387.1"
                     /translation="PDETLPPPVSTPIATPRMVHRTPIIPPAVHPPHAIPHPGPISRS
                     PEIVASVTATSTVGSYPHAPTAPPLAPQPASLALPNRPTWPNTADLTSPRSTTTHPIV
                     HPTTASVPVFAPPASATTVRSQRGDYRVGPRVGAGAFGAVFECLGPFDQRYALKVLRP
                     SGRPIERVREDWASEVRRLLQLRHPNVVYIHDAFEWEGSFCVALEWCDHTLHDMLEQP
                     LQEELAIELSRQMLAAVQYLHDWDIVHDDLHPGNVLIQQADRPIVKLSDFGISAELRG
                     APTVRPEIVHHAIMAPELIAAGYTSKQSDLYQLGMMMYWMITGSPPLDRNIPYEMLVH
                     QISEGVARQRAEQLATPFGNIVAKMLRRRDAYRYSSAREVWMDLRALPAWQARQIFRL
                     R"
BASE COUNT         1947 a         2474 c         2823 g         1924 t
ORIGIN      
        1 gagcaggtca gctccaagtc ccgtaccgaa ggtgtctcga ttgacaccca ccacaccggt
       61 catcgctcct ccatacgggt ccagagcaga gggggaatta tgcgtttcga ccttgtaaac
      121 gaggtgataa tcgtcgttga acgcgatgac cccggcgttg tcgtggaata ccgagaccag
      181 ccagctttct ccccgccgcg tttcctgctg ttttcgaatt cgttccgtgg gctcccggat
      241 ataggttttg aacagcgatt cgatggtttt ggactcttgt ccctgctcgt agtagtcgat
      301 ggtggcattg aaaattttgt gcttgcagtg ttcgctccac gtttgggcga gacactcgag
      361 ttcggcatcc gtagggaagg cgccgagacc cacgaccgtt cgcgctcgca aaaaatccgg
      421 ctcttcataa gcctcgcgaa tcgctttcat ctcctcgagc gatagcgaca ggatgcgttc
      481 tttggagagt cgttctagct tctgatccga tccggataga tcgatcgctc cacaaaccgg
      541 tgtatgagtg ctattgataa caggaataga cagatcaatc ggacttgcca gataatcgga
      601 ttgggatagt atgaagaacc gttcgatcaa ggagttggca aggagttccg tcgcaatccg
      661 gtgggcctgt tgttcgttca ccccggataa aaaggtcaga cgcgaggtaa aaacggcgga
      721 ggatggtgcc aggctgcgac ccgccatgtc ttggagcaaa accaacgcgc tctttccaac
      781 ggcatcggtc acgccgggcc gataaccaac actcacaacg aaatcgaagg gcgcgggagc
      841 caatcgaccg accgcgccct gttgggtgac cgggtccgca aggtgatcgc atgcgcgaat
      901 gacttcctgc tccgatagat ccgcctcgat cgtgtacacg tcgaaacagc gaacctcgcg
      961 aatcggaatg gatagaaacg agcggatggt cgatagggtg tccatccccc gtgggtcggg
     1021 gaagccaggg cgataaccaa cctcgatgcg atgggtcatg gccgcttgct agcggagctt
     1081 tcggcagagg ggaaccatcg aaagacgagg tttcgaacag gaaaacaagt gatattgcaa
     1141 agtcaccgtg agccaggaat ctggcgggat tcataaagat cgggaactcg aaacaatggc
     1201 ggttgtcgct tccacccatg ggacattgga acatgccgct gctcgcggtg tgcgtcgcct
     1261 cattgtcttc ggcggccacc gcttctccta tcacctccgt cgctccccat gccttggatg
     1321 agaccagccc accgcccgct caatgcgtgc aagcatcgca atcgatgcat ccatcccgcg
     1381 atgacacctt gatcatcacc ctcgacaacg agtgcagcgt cgccgtgaat tgctccgtgt
     1441 cgtgggtggt tcgttgcaag gatggtcagg agcattccta ttcccgttcg gcgcgtttgc
     1501 catcaggcga aaagcgctcg ttcgaagcgt ccgcatccgt ttgttccgaa gctggttggc
     1561 gcatcacgcc accacgatgg gaatgccaat caaagtgagc gatcagttcc gaacgagaat
     1621 cgcgcgggca ggtccaccat cgccatcctc cagcttcaga ggcaaacaga aaagctcata
     1681 atcacccggc tgcaccagcg tcaggtccaa tccttcgagg accaacaccg cgggatgccg
     1741 cgtcaataac attcgatgaa ccggcatcgt cggactatcg aacgcttcca cggaaggcga
     1801 atcgatgccg atcaaacgaa ctccttgcga tcgcagccag cacgcggcat cgagggtcag
     1861 gtgagcgtat cgtcgatcga acggacggtc cgcccagccc tcgctgtccg tacgcaatag
     1921 ccatcgctcc acgccaagac catcgcaacc gcgaacaacg gcttcatcga tacgacaacc
     1981 ccgccctcgc aggtcaaaga ctcgcgcagg accgcaaagc gttgacaggt cgagccgatc
     2041 cacgctttgc ccttggttgt cgaaatggcg aggagggtcg atatgcgtgc ccgtatgcgt
     2101 gccgaaactg agcgcagatg tattgtaagt atctaaattt gcttctattt tttgagtgag
     2161 gggcctgcag cgaaacggtg gatccccctc gtacacggtc atcgccggac gaagcgcaag
     2221 ggtgatgtcg atgatttgcc tggccatggg tgaactcact ggattggaaa cccactattt
     2281 gtagcgcgat gcgtggattt cgagcctggg atcgaactcg aagcgtggag atacagactc
     2341 acgacaaccg tttttttggc gtggtacgtc ttcagaacgc ttaccaatcc gtcgattgcc
     2401 gactttgcct caacaactcg accagcctgg catgcaaatg cccattggtc gccacgatat
     2461 gtccgtccag cacattgtcg ggctgcccat ccagcgccgt cgccatgccc cccgcagccc
     2521 gaaccatcac cacgcccgcc gcgatatccc atggccggag ttttttctcc caatagccat
     2581 cgtaggagcc atcagaaacg agacaaaggt cgatagccgc tgaaccacat cgtcgaatcc
     2641 ctaccgcgcg tttctgtaaa tatgcgaatt ctcgtatatt attatcgggg ctcgtctgcc
     2701 gatcataagg gaatcccgtg gcgagcaggg catcgtcgat gctggaccga tcgcttacgc
     2761 tacagggctc accgttgcga agcgtcaggc caccttccca gcctatccat tcgatatcga
     2821 gggcgggcgc gacgacagcg ccgacgaccg ggactccttg ctcgatcagg gcgatggata
     2881 cggcccaaaa cggatggcca tgcgcgaagt tggtggttcc atcgatgggg tcggcgcacc
     2941 aatagcggtc gtgggaggcc gaaccaccac cttcctcggc cacgataggg atggttgggt
     3001 ccgcggattg caaagatttt cgcaaggcat cttcggcggc gaagtcgagg ggggtcacca
     3061 gttcggcccc ttgtttgcgt tcgattctcg cgtgggagcg gaaccctttg agggccactt
     3121 gccctgcggc atgggcggcc tcgcgagcga taagggcatg atgtcgagct tgtcgtggcg
     3181 tcatggtcat cgggaaaggg cctggatcaa tcggttgtgg ataccgccga aggcgccgtt
     3241 ggacaaaagt gcgatggtat cgcctgggcg ggcttcgcga acgagcaagt cgatgatttc
     3301 gtcaatgctt tccggatggc gtgcccactg tccctggcgt tgaagctcgt cgatgatgcg
     3361 agggacatcg agccgttcgc tttgcgcgat atcgggacgg cccaagggtg caatcaccac
     3421 gtgggaggcg gcagtgaagg cttgggcgta agcatcttga tggagggcac ggcacgccgt
     3481 ggcacttcga ggttcaaaaa cagcgaatag ggagccttgg gggtggcggg cgcgcagccc
     3541 tcgcaaggtt tcctccaccg cggtggggtg gtgcgcgaaa tcatcataaa tattgatacg
     3601 attgggagtc cccaccaaat cctgccgtcg acgtaccccc tggaatcggg tcaacgattc
     3661 gcggacctga tggacggaca caccgaagcc ttgcatggcg accgcggccg cggcgatggc
     3721 attgctcacg ttatacgagc cgggcatggg cagcgcgaac cgaccggaag ccacgccccc
     3781 gatgaatagg tcgaaggctt gaccggtagc atccgctccc gcaagggcac caaggtaatg
     3841 gggcgtgcaa ccatgccaat tttcaccttg taatgcatac caaatcactt cacagcgggc
     3901 atgctggctc accacatcca ccacagtctg gctcgcagcc gaggccacca acaatccgct
     3961 gctgggaatc cgctccacga acgtcgcgaa cgcatgaaga tacgcctcga acgtcggata
     4021 aatatcgatg tgatcgtatt cgatcgacgt gatgacggcg atttcgggcc gatagtgcca
     4081 gaattttggg gttttttcga aaaaagccgt gtcgtattcg tcgccttcaa tcacgaaagg
     4141 cgttcgacgg gtcggcatgg gatgatcggc caacggcaag ccaggacctt ttcgagaggg
     4201 gatacgaaaa ctgtgttcga agtttttggg caacccgccg atgagcatgc cagggtcttt
     4261 gcccgcgtcg aataacagcc acgcggccag ggaagaagtc gtggtttttc catgagttcc
     4321 ggcaatcacg accggcgtct tcgcctcgag cacgaggtcg gcaagaccgt gggccatatc
     4381 cgtatacggc aggccgcgat cgatggcagc gcgcgcttcc gggttgttgg atcgacaaac
     4441 gttgccgatg acgacgagat ccggggcggg gtcgaggtgg gcgggatcga agccctgcat
     4501 gaggcgaata ccccattttt ccaaagcagg tcccatggga gggtcgaagc gcacgtcgga
     4561 gccggaaaca tcatgcccaa gctctttgaa caaacccgcg agggcaccca taccggttcc
     4621 agcgacagca atcagatgca cgtgcatggg gctgcacgtt gtatccgttt tggacggctg
     4681 gcaagctttc gtcatggttt accgaagagg ccggagggcc ggatcgagag ccaacggaag
     4741 cgaaggtggg aaaaggtgag ttggcaggtt gttggggcga gacaactcac gtataattca
     4801 cgaatccgat agcacggggc tcaaacactg caaatcatat gtgatttcag catgttacaa
     4861 atatttcatc cggatgggag gagacctacg agatgcaaac gctggcgcat tatcccaaaa
     4921 cggtgtccat cgtcgatggg caggtggtga ttcgtccgat ggcgcaggag gattttgatg
     4981 gtgtacggag gtttttcgag cagttgccac ggcaggatcg gctgtttctt cggcacgacg
     5041 tgcttcgacc ggaggtattt tcccgttggt ttgcggaatt ggattacggc aagaagttgc
     5101 cgttgatggc gctacatggg gagcaaatcg tcgggcatgc gttgctcgat ggcgagcagc
     5161 gcagttggtc tccccacgtg gcggaaatcc gggtggtggt ggccgatgcg ttcaaaaagc
     5221 gggcggtggg gacggtgttg gcgcgggagc tattcgatca agcgatggcc cgggggtatg
     5281 agaaagccat ggcgagcatg atggactcgc agaaagccgc tcgcaggatg ttcgagcgca
     5341 tgggtttccg tgtggaagcg gaactcaagg accatgtcaa agacctggat ggcaagaagc
     5401 acaacctcgt tctcatgtca tgcatgctgg aggatgcgtg ggaaaagatg tccgagttgc
     5461 tgcaggactt ctcgccctgg gtgggctggc attgaggtga cccaaccgat gaagcaatgc
     5521 gctgcgcgca tgcagcaatc ctttgcgtgg gtgcatcgaa accgatcttg ccgcaagtgc
     5581 gatccttgtg gcgttagtca gcgaaaagtg gcactgagtg ttctcagttt cgttctcatg
     5641 cgtccacgtc atacccatcg aattcagctc accccacgac gtttcgagcg agggacccat
     5701 ggctgctagt cgtccaaagc gaccgtctgg aaagctcgca acccttgtcc ttgcccttct
     5761 tgctgtgggt ttcgccagca gtgtgtatct gacgaacctg tatatcgagg ccacggaagc
     5821 cttgaaggga gggaccaaac tcgacagttt ttgcaacctg agccaggggg tcaattgtga
     5881 aaccgtagcc gccagcgagt attcggcgtt tttgggaatc cccatatccg tatggggact
     5941 cgagtatttc ggtcttgcga tcgcgatcgt gctcgcctcc cactatcgtc tgctttcgct
     6001 aaagcgatgg gattcgctgc tattcgcgct gtcatgcacg ggcgtgcccg ttaccatcgt
     6061 tcttggctat atttcggtca ccatcattca atccgtctgc atcatgtgcg tgacggtcta
     6121 tgtggtgaac accgcgatgg tgttgttgct ggcggggctg aaccgcagcc gactgaaaga
     6181 gttcgtgcga gaggggccga tggaactgtt ggcgctggcg acccacgcca accatcgggt
     6241 cgtcacggtg tttgcggtga ttctcggtct atcgcaattc ctatgggttc caaggctttt
     6301 cgcacgtccg acccatggtg aggatgcagc ctcgacattg gccgcggtat ccaacgccaa
     6361 gacaggaacg tggaaagaat taccggcatc gcgtaacacg ctggggcctg tgaatgcgcc
     6421 gattcgcatc gaggaattca cggacttcca atgtcctttc tgtggcaggg cccacgatgt
     6481 gctgctcgag gtggtgacgc gttatcccgg taaaatccac ttgattcacc gggactttcc
     6541 gctcgatcac cattgcaatc cggcgattcc ggagccgttc catcccgatg cctgtggagc
     6601 ggcttacttt gcgcgatgcg ccgcggatca agacctgttc tggccgtacg aggccctgct
     6661 ctttcacaac caacgaaagc tgaaaaaagc ggccatgcta tcgttcgctc agaccgtggg
     6721 attggatacc acggcattgc aaacctgcgt cgaatccaaa gtcacccatc aggccgtgct
     6781 tgcggacatc gacgaaggga tcaagcgcgg agtggccgga acgccagcgt ttttcgtcaa
     6841 tggcgaagct atcgtcggcg cacgtcccat cgagttttgg gaagaaaaaa tccaaacctt
     6901 gttgggcttg gctccgcctg cttccgcttc ttccgcaacg ctttcgacca gcgccgcccc
     6961 tccatcttcc gcgcttccgg ccatgagtgc agcgccttcc gccagcaacc cgcacccctc
     7021 ccccgcggct tccgcatcgg tcaccccatc gccttgaatc cttcgcggct tccgcaagga
     7081 cgcacgggat tcatgtcgag gctcccagcg atttggattt tctcaagcct gtcccggatc
     7141 tgatatttat acaataaaat cagttagtta cactaatatc cgaaaatgga tgagcggtgc
     7201 gcaacggtcc gttttgccgc ttcggctcta gggttccgtc gtcacgcgga gggattcgat
     7261 caccacatct tcgacgggca cgtcttcgta gatgcccacg gtatgcgtgg ccacggcttc
     7321 gatttcgtcg actatgtcca ttccttccac gacgtttccg aagacggcat accccgcagg
     7381 cgaggattgg cctgggtcga ggaacgtatt gtccacggta ttgatgaaga actgggaggt
     7441 agcggaatcc ggctggttgg tgcgtgccat ggcaatggtt ccgcgaagat tggacaggcc
     7501 cgaggcggaa gcttcattga cgatgggcgg gaaaaggggc gcccgtgttt tcatacctgg
     7561 ctccatgcct cccccctgaa tcatgaagcc ggcgatcacc cggtggaaaa tcaatccatc
     7621 gaaaaactgc gcgtcggcat agcgctggaa attctctacg gtgatgggag aagcatcatc
     7681 agcaagcttc acacgaatcg ttcctttgga cgtgacgatc gtcgcgaaga gcgaaggttc
     7741 tgaggattcc gcgggcacat ccggcgacac gtcgggggag acattttgat cgggggtgac
     7801 atcggcatga ctgtcgggtt tggcgtcggg cggagccatg tcggttgccg catcatgggt
     7861 ggtttccgca tcgcgttgcg tggagtcctc ggaggtggag cagccgaaaa atccgaaaga
     7921 ggcaatgaaa aagctggcaa aaagcgtgcg catgaagacc tcgcgggaca aggggttggg
     7981 taaggatcaa cgaaggcgga agatctgtct tgcttgccag gcgggcaagg cacgcaagtc
     8041 catccagact tcccgagccg atgaatatcg ataagcatcg cgtcggcgca gcattttcgc
     8101 aacgatattc ccgaatgggg tagcgagttg ctcggctcgt tgtcgagcga cgccttcgga
     8161 tatttgatgt acgagcattt cgtaggggat attgcggtcg agaggtggcg agccggtgat
     8221 catccagtac atcatcatgc cgagttgata cagatcgctt tgtttgctcg tatatccggc
     8281 ggcgatgagt tcgggagcca tgatggcgtg gtgtacgatt tcggggcgta cggtgggagc
     8341 gccgcgcaat tctgccgaga tgccgaagtc ggagagtttg acgatgggcc ggtcggcttg
     8401 ttggatgagc acgttgccgg gatgcagatc atcgtgaacg atatcccaat cgtgaagata
     8461 ctggacggcg gcgagcattt gccgggataa ctcgatggca agttcctctt gcaagggctg
     8521 ctcgagcatg tcgtgcaagg tatgatcaca ccattcgagg gcgacacaaa acgagccttc
     8581 ccattcgaac gcatcgtgga tgtatacgac attgggatgg cggagttgga gcaagcgccg
     8641 gacttcactg gcccagtctt cccttacccg ctcgatggga cggccggaag gtcgaaggac
     8701 cttgagggcg tagcgttggt cgaacggtcc gaggcattcg aagacggccc cgaaagctcc
     8761 cgcgccaacc cgtggaccca ccctataatc acctcgttgg gaacggacgg tcgtggcgga
     8821 agcgggggga gcaaagacag gtacggatgc ggttgtgggg tggacgatcg gatgcgttgt
     8881 cgtggaacga ggcgacgtca ggtcggcggt gttgggccag gtcggtcggt ttggaagggc
     8941 aaggcttgcg ggttgtggcg caagcggagg tgcagttggt gcgtggggat aggaaccgac
     9001 ggtggacgtg gcggtcacgg aggcaacgat ttcgggcgag cgggagatgg gcccgggatg
     9061 ggggatggca tggggtgggt gaacggctgg agggataatg ggggtacggt ggaccattcg
     9121 cggggttgcg atgggagtgg agacaggagg cggtagggtt tcgtcggg
//
LOCUS       DAOSOR010000185         9141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_7544_length_9141_cov_3.870790, whole genome shotgun sequence.
ACCESSION   DAOSOR010000185 DAOSOR010000000
VERSION     DAOSOR010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9141
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7544_length_9141_cov_3.870790"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PK710_11490"
     CDS             <1..729
                     /locus_tag="PK710_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOT10388.1"
                     /translation="GGSGGCTAVGHDEDFDLVDDGCDNCPSFANQSQKDTDGDGLGDA
                     CEWHNNSDALSKVMYFEPFFESGWASSWDLNSSYKVGTDVLNVNNENCSSGNCGKNSY
                     WKTEISGLTPYAVETSLLYGSGSFGWAGVLLGESPGQNKWWGCFLDREQYSRTLELWE
                     NTGSSLVRRQWKTSVENTSTGSSVQRWLRAYWNGSSITCEYRNGNGAVAQTQPYSVSG
                     TMTGRAGVRVYSVNAEFHSFTVYR"
     gene            1086..2612
                     /gene="menE"
                     /locus_tag="PK710_11495"
     CDS             1086..2612
                     /gene="menE"
                     /locus_tag="PK710_11495"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HOT10389.1"
                     /translation="MAELNVNIGHWIRQNAIIYPHKIATKTGDRSFTYRQLAERMNRL
                     AHALIASGLKPGDRVALYLLNGYEYVEAVFACSAAGLIAVPLNWRLSQEELVFIMKDC
                     QPKALVFHAMFEQQAALLAAECPSVSLSLSVAHDGGPSPYESYLQTGDPSDAVNPAVG
                     GDDPHLIMYTAGTTGTPKGVILTHANCFWQSVNGWALGVSPDTVALVILPMFHVGGLN
                     GCVTPMIHIGATVILSPKFDPADVLETIERERVNGMVAVPAIYQMLYDHPKFNTTDLS
                     SIVAFISGGAPLGKELTRKYHERGLEFRQGYGLTETSPGVTGMGPGECLKKSGTAGRT
                     CTYVDVRIVGEDGKDLPKNEPGEVVIRGPNVMKGYWNRPEATAEVLRDGWFHSGDIGF
                     LDDDGFLTLVDRKKDMIISGGENVYPAEVEKVLLEHPAVAEAAVVGKPDEKWGEVPIA
                     FVSPVPGQTLDAQELLHFCSSRIAKYKTPKQVLIRDSLPHSAAGKILKQQLKREVREG
                     "
     gene            complement(2636..3565)
                     /locus_tag="PK710_11500"
     CDS             complement(2636..3565)
                     /locus_tag="PK710_11500"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT10390.1"
                     /translation="MPTPLNSSTDEVKSAPVVPISGGVAQENISLSAVFMVLGAGFFV
                     ALAALVKAASDEAAPMQAVFYRSVFSALPLLGIMRMQRVSVVGTRMKLLVVRGVLGFL
                     ALFCYLWAVSHIPLADVLALQQTNPVFVAFLAVWLLGERPRRSHYLLAGVCLIGAWLV
                     VRPTRGVASLDSLMALISALFSSGAYVTVRALTRTETTQRIVLWFSLVAAALSLPFVA
                     ADWHPLSLRSHGLLVGAGLLALLAQSFMTASYRRAPAHLASAFSYASVPLAYLAGLIF
                     WNERPDSLSHLGITLIVAAGIVLVVRMRPSGST"
     gene            3763..5175
                     /locus_tag="PK710_11505"
     CDS             3763..5175
                     /locus_tag="PK710_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10391.1"
                     /translation="MAPSITTSARQLAADWVGNSTALTFRNSRDSLKLLRMMSYVTHQ
                     TMEGSVFPAFEAALGMQEAFSGSVVAALRGDRKALEVAKESVDRVRAAAKYGKLVRRW
                     GKQLYGSATFEGEQVLSQGPFLQLSYLPPPTTEKQAPFALFHAGGGIPYGDRIFRFLP
                     ESNFFDRFLERGIPVYAVELRGDRKQIDYAGLTLEHLIEAIDSLSAIAFEHHGQRKMI
                     LEGYCGHGMQTLAFVAAKPRQATERFHAATTFVSPYDGRECSILAEFPSLMPEAMMDL
                     SFAIAKLIGQGYVPGDGMRMSLDLGLRTTFHKTPLAHVAAGWNETTYAKVRTVEDLSK
                     RQRRDLAGAYWISPESARRFALPIDLVRYSSRLFTKGIGPDGELPASYQGKSLSLKTI
                     LDDTPLPIIGFFGGRDVMIPDRTAFPILSLFGKRYRHIVHPQAGHISYVLSPKSWQIG
                     HKTSLEPNPIDVLHEVVAAS"
     gene            5288..6082
                     /locus_tag="PK710_11510"
     CDS             5288..6082
                     /locus_tag="PK710_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10392.1"
                     /translation="MRLLPYLLIGALLPLLPSCKACERESDEPPPLPPATTVTPEPTA
                     PATLTTVEPPKPGEIVRPELEDREDGITGKAITVTGAKANLQVPNDWNVAKADVQTAT
                     AADQKSRMGVTNYGAEGHSAALDKAMASTGLSGCTWQPTQSFSVGKDKLMAQAADGLC
                     QRNGAQVSTAFMATEGLLALGSWDEGADRTALFGSMRSVAKVKVGTGTSNLVACCRAL
                     AQNAKSQPPPQSGFMLQAAAACEAAARNNDASAVNAALRQFGMRCN"
     gene            complement(6254..6766)
                     /locus_tag="PK710_11515"
     CDS             complement(6254..6766)
                     /locus_tag="PK710_11515"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HOT10393.1"
                     /translation="MSRFKFLQAMFMVLGLVTMLGCSKPKPPQLTPEATSVTSVSGKG
                     IGIRVVLAAYNPNDFDLNTRSVEGTIKLGDTVTLGPITKPHGVKLPANKSTNVELELE
                     ATWGQAVQLAQLATSGPQVPYVVEGKVTIGGETLNVDLPFTIKGEVSQTQLIAAGLKG
                     LPPIPGLPLR"
     gene            6789..8276
                     /locus_tag="PK710_11520"
     CDS             6789..8276
                     /locus_tag="PK710_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dickkopf-related protein"
                     /protein_id="HOT10394.1"
                     /translation="MALTIPSVAPHPKNGAGVDPSSPTAMSHGTLCRWRLRMLPRIVA
                     CVVVGWALAGCSADSDSQRNYGHGGSSAAGASGGGGGTLDSGTTVPEADTPDTLSVNP
                     EASTDVFVPDGDSCLKTSAAAEPFPLDMYVMMDQSGSMSMPVNLMQPLGETQWGAVKK
                     AFVGFLNDPATPTGMSMGIQYFPLPIAPWEPLECSSETDCASGSICVKLDNGKHCVAK
                     CTSDANCPAVSECMSFDDGNGGTLKFCSNDSCDVSAYATPEVPIAPIPSVNAEILASL
                     NAHSPLTMTPSAPALEGAIQYARSWATQHPGHTTIVIFATDGQPTACSKAAFPINEVK
                     SIAAAGVNGSPSIRTFVIGVLSAGAGPLGNSLHEVAKAGGTNQALIVQVNQDMTAQFK
                     AALEAIRGAFMQCEFQVPNPGIPMDYDEVNVVYTAPNGEKFPVYYVGSLADCDPNMGG
                     WFYDTDPKIAEPTKILLCPQSCQFMQAHSGSIDIEIGCKTITPPK"
     gene            8426..>9141
                     /gene="phnD"
                     /locus_tag="PK710_11525"
     CDS             8426..>9141
                     /gene="phnD"
                     /locus_tag="PK710_11525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOT10395.1"
                     /translation="MHTLRRYVLALPLLLWIVVATSACKKQSTADAATSFVFVLSPSY
                     HAEPAKIAAMQKYVEENSGLKLEIRMADSQANAVASVGAATADAWLLPLFDYLFCHQE
                     YGVHAGLRLMRSGTGSFHGVIVTRADGPIEKLDQLAGKTIAFVDRYSTSGFVYPAKSL
                     RQAGVKPTTVFAQSHKNALEQLRTGKVDAAATYARAVEGDTSLRVIATTDPIPNEPIF
                     FRSDLDAEKRDKFIRAMTSFA"
BASE COUNT         2045 a         2721 c         2473 g         1902 t
ORIGIN      
        1 ggcggcagcg gcggttgcac tgcggtgggg cacgacgaag atttcgattt ggtcgatgat
       61 ggctgtgata attgcccgtc ttttgcgaat caaagtcaaa aagataccga tggagatgga
      121 cttggagatg cttgtgaatg gcacaacaat tcggacgccc tatccaaagt gatgtacttc
      181 gagccttttt tcgagagtgg atgggctagc tcgtgggatc tcaattccag ctataaggtt
      241 ggtactgatg tgctgaacgt aaacaacgag aattgttcct ccggtaattg cgggaaaaac
      301 tcatattgga aaaccgagat ctcgggtttg actccctacg ccgtggaaac ctcgctactg
      361 tatgggtctg ggtcttttgg gtgggccgga gtgcttttgg gggaaagtcc tggacagaac
      421 aagtggtggg ggtgtttttt ggaccgtgaa cagtactcca gaaccctgga gttgtgggag
      481 aatactggca gtagcctcgt tcgccggcaa tggaaaacga gtgtcgagaa cacatctact
      541 ggatcttccg tgcaaagatg gttgcgagcc tattggaatg gctcctcgat cacatgcgaa
      601 tatcgcaatg gcaacggcgc tgtggcgcaa acgcaaccgt attccgtgtc cggcaccatg
      661 accggacgcg ccggtgttcg ggtttatagt gtcaatgccg aattccactc tttcacggtt
      721 tatagataat caaatattat cataaatttt agatatttac ctctcgaccg cgaaggctcg
      781 cgttgttgca ctgcacaatt ctgcccttga cttttccctc ccctcgtcta ccctgcgtcc
      841 gcttgggaat ggctttccga agtcgaccgg cgcgtcgcac aacgcgatca aagcctcaga
      901 cgaccatgtc gtcacggctg ccgctcgatg gatcacgctt tgaaacgtta ccagaacatg
      961 atgcgccaca tgggatgcca agaccgaaaa ccgcatagac aaggcaatga catcacgttc
     1021 gggcgtgcga aacgcgatcg ataggaaccc ggggccgaca ccagcaaaga cggaggcgca
     1081 agagcatggc agagttgaac gtcaatattg gacattggat ccgtcaaaac gcgatcatct
     1141 atccccataa aatcgccacc aaaaccggcg accgctcgtt tacctatcgg cagctcgccg
     1201 aacgcatgaa tcgtcttgct catgcgctca tcgcatcggg attgaagcct ggagaccggg
     1261 tcgccctgta cctgctcaac ggctacgaat acgtggaagc cgtctttgct tgctccgccg
     1321 ccgggctcat cgccgtgcct ctcaactggc gtctcagtca agaagaactc gtgttcatca
     1381 tgaaagactg ccagcccaaa gctctcgttt ttcacgccat gttcgagcag caggccgcct
     1441 tgctcgccgc cgaatgcccc tcggtttcgt tgagcctttc cgtcgcccac gatggaggcc
     1501 catcgcccta cgaatcttat ctgcaaaccg gcgatccaag cgatgccgtc aaccccgccg
     1561 tgggcggtga cgaccctcat ctcatcatgt acaccgccgg taccacggga actccgaaag
     1621 gcgtcatcct cacccacgcc aactgcttct ggcaatccgt caatggttgg gcgctgggcg
     1681 tgagtcccga taccgtcgct ctcgtgatcc ttcccatgtt ccacgtgggc ggcctcaacg
     1741 gatgcgtcac acccatgatc cacatcgggg ctaccgtcat cttatcgccg aaattcgatc
     1801 ccgccgacgt gctcgaaacc atcgagcgtg agcgtgtcaa tggaatggtg gccgttcctg
     1861 ccatttatca aatgctttac gaccatccca agttcaacac gacggatcta tcttccattg
     1921 tagcgttcat ttcggggggg gcgccgttgg gcaaagagct gacgcgcaag taccacgagc
     1981 gcggcctaga attccgtcaa ggctatggat tgaccgaaac atcgccgggc gtcacgggaa
     2041 tgggaccggg cgaatgcttg aaaaaatccg gaaccgcggg acgcacgtgt acctatgtgg
     2101 acgttcgtat cgtgggagaa gatggcaaag acctgcccaa aaacgaaccg ggagaggtcg
     2161 tgattcgcgg accgaacgtg atgaaaggct attggaacag gcctgaggct acggccgagg
     2221 tgcttcggga tggctggttt cattcggggg atatcggatt cctcgacgac gatggatttc
     2281 tcacgttggt cgatcgcaag aaagacatga tcatttcagg aggggagaac gtgtaccccg
     2341 cggaggtgga gaaagtcctg ctcgagcatc cggccgtcgc ggaggcggcg gtggtaggaa
     2401 aacccgatga aaaatggggc gaagttccta tcgctttcgt ttctcccgtg cctgggcaaa
     2461 cgctcgatgc gcaggaattg cttcacttct gctcgagccg cattgcgaag tacaagacgc
     2521 cgaaacaggt attgattcgc gactcgctgc cgcatagcgc cgcgggaaaa atcctgaaac
     2581 agcagttgaa acgagaggtt cgcgaaggat aaacccaccc ttcgcactcc ctccgctacg
     2641 tcgagcccga cggtcgcatc cttaccacca gcacgattcc cgctgccacg atcaacgtga
     2701 tgccaagatg cgataggctg tccggtcgct cattccagaa aatgaggcct gccaggtagg
     2761 ccaacggcac gctggcataa gaaaacgccg aagccagatg cgccggtgct cttcgatacg
     2821 acgccgtcat gaagctttga gccaagagcg cgagcaaccc agcgccaacg agaagcccgt
     2881 gggatcgtaa cgaaagcgga tgccaatcgg cggccacgaa cggcaacgaa agcgcggccg
     2941 ccacgagcga aaaccacaat acgatgcgct gagtcgtttc ggttcgtgtc aacgcgcgga
     3001 ccgtgacata agctcctgac gaaaacagag cagaaatcaa ggccatcagc gaatccaggg
     3061 aggcaacgcc ccgcgtggga cgaaccacca gccatgcgcc gatcaggcat acgcccgcaa
     3121 gcaaataatg actgcgacgg gggcgttccc ccagcaacca aaccgcaaga aacgcaacga
     3181 atacggggtt cgtctgttgc aacgcgagta cgtccgccaa cggaatatgg gaaaccgccc
     3241 acagataaca aaacagggca agaaaaccca gaaccccgcg aaccacgagc agtttcatgc
     3301 gcgttcccac gacgctcaca cgctgcatac gcatgatgcc gagcaggggg agagccgaaa
     3361 atacggaacg gtaaaaaacc gcttgcatcg gcgccgcctc gtcggacgcc gctttcacca
     3421 aggccgccaa ggccacgaaa aaacctgccc ccagcaccat gaacactgcg gaaagcgaga
     3481 tgttttcttg cgcaacacca ccgcttatcg gaaccaccgg cgcggatttc acttcgtcgg
     3541 tggaggaatt cagcggcgtg ggcacggcga aaatgggtgc tttctccccc gcttttcgct
     3601 acccccctcc aaccccaagg tcaaggaaga ttcggttttg ccatgcagca tccatggccc
     3661 ggccttggct tccgcctctt gacaagaacc agattatcat cgcatgaaga tagaggccaa
     3721 tattgtgcgc cgcacaatca tgagcgtcga cgagaggagt tcatggcacc atcaatcacc
     3781 acttctgcac gacaactagc cgcggattgg gtgggaaatt ccacggcctt gacgtttcgc
     3841 aactcccggg actcgctgaa actccttcgt atgatgagct atgtgacgca tcagacgatg
     3901 gaaggcagcg tatttcctgc cttcgaagcg gcgttgggga tgcaagaagc cttttctggc
     3961 tctgtggttg ctgctcttcg aggtgatcgc aaagccctcg aggtggcaaa agagagcgtg
     4021 gatagggttc gtgcggcggc aaaatatgga aaattggtac gacgatgggg taaacaactc
     4081 tatggatccg cgaccttcga gggagaacag gttctttccc agggaccttt cctacagctt
     4141 tcctatttgc cgcccccaac gactgaaaaa caagcgcctt tcgcactttt tcatgcggga
     4201 gggggtatcc cctatggcga ccgcatcttt cgtttcctgc cagaatccaa cttcttcgat
     4261 cgctttctcg aacgcggcat tccagtgtac gccgtcgaat tgcgcggcga ccgcaagcag
     4321 atcgactacg ccgggctgac gttggagcac ctcatcgagg ccatcgattc tctatccgcc
     4381 atcgctttcg agcatcacgg acaacgaaaa atgatcctgg aaggctattg cggccacggc
     4441 atgcaaaccc tcgcgtttgt cgccgcgaaa cctcgacaag ccaccgaacg cttccatgcc
     4501 gccaccacct tcgtctctcc ctacgatggt cgagaatgct cgattcttgc cgaattcccc
     4561 tcgttgatgc ccgaggcgat gatggactta tccttcgcca tcgccaagct cattggacag
     4621 ggatacgttc ccggcgatgg catgcgtatg tctctcgacc tcggattgcg aacgaccttc
     4681 cacaaaactc cgctcgccca tgtcgccgcg ggctggaatg aaacgaccta cgcgaaagtc
     4741 cgcacggtcg aggacctgtc caaacgccag cggcgcgacc tggccggagc ttattggatc
     4801 tcgcccgaaa gcgcgcgtcg cttcgcctta cccatcgacc tcgttcgcta ttcgtcgcgc
     4861 ctgttcacca aaggaattgg ccccgacggc gaattgcccg cctcctatca aggaaaatcc
     4921 ctgtctttga aaaccatcct cgacgataca cccttgccga tcattggctt tttcggcggc
     4981 cgggacgtca tgatccccga tcgaaccgcc tttccgatcc tttctctttt cggcaagcgt
     5041 taccggcaca tcgtccaccc ccaggcgggg catatttctt atgtcttatc gccaaaatca
     5101 tggcaaatag gccataaaac ctcccttgag cccaacccca tcgacgtcct gcacgaggtt
     5161 gtggcagctt cataatgccc cccaagcgtg gaaacatcga acaacaaacg cactgaaacg
     5221 ttgacatcgt cgtcgttcaa gcattgaatc ttcatcgaac atcttggcga tgtcctcgga
     5281 ggataccatg cgattgcttc cctacctgtt gattggcgcg ctgctgcccc ttttgccctc
     5341 gtgcaaagca tgcgagagag aatcggatga gccgccgccg ttgccgccgg ccacgacggt
     5401 cacgccggaa cccacggctc ctgccacctt gaccaccgta gaacccccca aaccaggcga
     5461 aatcgttcgc ccggaactcg aggatcgaga ggacggcatc actggcaaag cgattaccgt
     5521 caccggggcc aaagcaaact tgcaagtccc caacgattgg aacgtggcca aagccgacgt
     5581 tcaaaccgcg actgcggcgg atcaaaagtc gcgcatgggt gtgacgaatt atggcgcgga
     5641 aggacattcc gcggcgttgg acaaagccat ggcttccacc gggttgtccg ggtgtacctg
     5701 gcaacccacc caatcctttt ccgtggggaa ggataagctg atggctcagg ccgcggatgg
     5761 tctctgccag cgcaacggcg cgcaagtcag tacggctttc atggccaccg aaggcttgtt
     5821 ggcgttgggc tcgtgggatg aaggcgcgga ccgtaccgcc ctgttcggct ccatgcgttc
     5881 cgtggccaaa gtcaaggtag gaacgggcac gagcaacctg gtggcttgct gccgtgcgct
     5941 cgctcaaaac gcaaaatctc aaccgccgcc gcaaagtggg ttcatgttgc aggccgccgc
     6001 cgcttgtgaa gccgccgcaa gaaataatga tgcttccgcc gtgaatgctg ccctgcgaca
     6061 attcgggatg cgctgcaact aaccctattc accgatccgc gcttccccat acgtcacttc
     6121 tccccttcgc cagcccaggc ctcgctcgac tccataaaat cctattccat gatcgtaacc
     6181 ttgcgtaatt caacctaaat ttagaaatcc ccccggaagg gaaagacggg gtcaaatgac
     6241 gtttcgatgc gatttaccgg agcggaagcc cgggaatggg tggaagccct ttcaatcccg
     6301 ctgcgatgag ttgtgtctgg ctcacttccc ccttgatcgt aaacggcaga tccacgttca
     6361 atgtttcacc accaatcgtc acctttcctt ccaccacgta gggaacctgg ggccccgagg
     6421 tggcgagctg ggctagctgt actgcctggc cccaggtggc ttccaactcc agctcgacat
     6481 tcgtgctctt gttggcgggg agtttgacgc cgtgcggttt ggtgatcggg ccaagggtta
     6541 ccgtatcgcc cagcttgatg gttccctcca cgcttcgtgt gttcaagtcg aaatcattgg
     6601 gattgtacgc ggcgagcacg acgcgaatcc cgatgccttt gccgctgacc gaagtgaccg
     6661 aagtggcttc cggggtcaat tggggcggtt tgggtttcga gcatccgagc atcgtgacga
     6721 ggcccaggac catgaacatc gcttgcaaga acttgaatcg actcatgcga cgattctagc
     6781 ggatagcggt ggcgttgacg attcccagtg tcgcgccgca tccgaaaaat ggtgcagggg
     6841 tggacccatc atcgcctacg gcgatgagcc atggtacgtt atgtcgttgg aggcttcgaa
     6901 tgctgccaag gatcgttgca tgtgttgtgg ttggctgggc gctggctggg tgcagtgccg
     6961 actcggatag ccaaagaaac tatgggcacg gaggttcttc cgctgcggga gcttctgggg
     7021 gaggaggggg caccttggat agtggcacca cggttcccga ggcggatacc cccgacacgc
     7081 tttccgtcaa cccggaggca tcgacggatg tattcgttcc cgatggtgat tcttgtttga
     7141 agacctcggc ggcggcggag ccattcccgc tggatatgta cgtgatgatg gatcaatccg
     7201 ggtcgatgtc gatgcccgtg aacttgatgc agcctttggg ggaaacccaa tggggcgcgg
     7261 tcaaaaaagc tttcgtcggc tttctcaacg atccggccac gccgaccgga atgagcatgg
     7321 gaattcaata tttcccgttg cccatcgcac cctgggagcc gctcgaatgc tcgtcggaaa
     7381 cggattgtgc ctccggttcg atctgcgtaa aactcgataa cggtaagcat tgcgtggcga
     7441 agtgtacgag cgatgccaat tgccccgcgg tatccgagtg catgagcttc gatgatggca
     7501 atgggggcac gctcaaattc tgtagcaacg attcttgcga tgtttccgcc tacgccaccc
     7561 cggaagtgcc catcgcaccc attcctagcg tcaatgcaga gatccttgcc tcgctcaacg
     7621 cgcatagccc acttacgatg accccgtcgg cccccgcgtt ggagggcgcc attcaatatg
     7681 ctcgctcatg ggcaacgcag catccggggc atacgaccat cgtcattttc gccacggatg
     7741 gtcagcccac cgcatgctcc aaagcggctt tccccatcaa cgaggtcaag agcatcgccg
     7801 ccgctggcgt gaatggtagt ccatcgattc gcacgttcgt catcggcgtg ctctccgcgg
     7861 gcgcgggacc tctgggcaat tctttgcatg aagtcgcaaa agcgggcggc accaaccaag
     7921 ccctcatcgt gcaggtcaac caagacatga ccgcgcagtt caaagcagcc ctcgaagcca
     7981 tccgtggcgc gttcatgcaa tgtgagtttc aagtgcccaa tccaggcatc cccatggatt
     8041 acgacgaagt caacgtcgtc tataccgccc ccaacggaga aaaattccca gtgtattatg
     8101 tcggttccct cgcagattgt gatcccaaca tgggcggatg gttctacgat accgacccga
     8161 aaatcgctga gcccaccaaa atcctgctgt gcccgcaatc ttgccaattc atgcaagccc
     8221 atagcggatc gatcgacatc gagatcggat gcaagacgat cactccgcct aaatagctaa
     8281 aataattcat ttaaattgag tcgcggccgc ctgctggaaa acctcttgcc gcttctcgaa
     8341 cccatcgatc ccaagcgttg tccacgatat gtccccgata gggttgccgc ctcgcgctcc
     8401 ccaggttaaa cgccccctgt tcaccatgca tacactcaga agatacgtgc tcgctctgcc
     8461 cttgctgctt tggatcgtgg tcgccacgtc agcctgcaag aagcaatcga cagcagatgc
     8521 cgccacctct ttcgttttcg tgttgtctcc gtcgtatcat gcggagcccg cgaagatcgc
     8581 tgccatgcaa aaatatgtcg aggaaaactc cgggctcaaa ctcgagatac gcatggccga
     8641 ttcgcaggcc aatgcggtgg ccagtgtcgg tgcggctacc gccgatgcgt ggctgcttcc
     8701 cttatttgat tacctgttct gtcaccagga atacggtgtt catgcggggc ttcggctcat
     8761 gcgttcgggc accggatcgt tccacggggt catcgtgaca cgggccgatg ggcccatcga
     8821 aaaactcgac caactcgcgg gaaaaaccat cgccttcgtc gatcgatact ccacctccgg
     8881 tttcgtttac cccgccaaat ccttgcgaca agccggtgtg aaacccacca ccgtcttcgc
     8941 gcaatcccat aaaaatgctc tcgaacagct tcgtaccgga aaagtcgatg ctgccgcaac
     9001 ctacgcgcgc gccgtcgaag gcgatacctc cttgcgcgtc atcgccacca ccgaccccat
     9061 ccccaacgaa cccattttct tccgttctga cctcgatgcc gaaaaacgag acaagttcat
     9121 ccgcgccatg acttcttttg c
//
LOCUS       DAOSOR010000186         9096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_7599_length_9096_cov_4.949784, whole genome shotgun sequence.
ACCESSION   DAOSOR010000186 DAOSOR010000000
VERSION     DAOSOR010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9096
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7599_length_9096_cov_4.949784"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="PK710_11530"
     CDS             complement(<1..1361)
                     /locus_tag="PK710_11530"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOT10396.1"
                     /translation="MTRNRLAKTIPRPNQMLRIVLPAFSGSLLFLSDYPAHLWPLQLF
                     AFLFWIGSLDSGPKKEGFRSAWLVPLGAGAILGVFYAAPLLVVTAFPWMMAIGLGIYT
                     VFGWMVASWIAYRVNRWPGLHGAFAVGAVVALVEWIATHAIPIWGTAQSFVRVWSESP
                     WAIQFVDITGGTGIVFVLVSAQAVVVRLLRKTLSPRRGLTSLAVMFGLITLYNAARWF
                     EPPISHLRVAAIGWVDDDMPLGARTPSPQVVETMVIPLVRKAVDQGAALVVCPEAPFM
                     TTTQTRDLLLEPLFMLAQQRDVALAIGYFDRTHDDNRVVLIDRKGAHPIEYRKTHRVP
                     FIERYQAGDGTRVDWQVRGNAVGAMICQDDNFEDLASGYGRDRRSIVLVPTNDWHAVK
                     DYHYENSRMRPIENRYGVIRAATNGISAVLSARGQVLAASDHFADGAGVVMADLPIYR
                     SSS"
     gene            1503..2759
                     /locus_tag="PK710_11535"
     CDS             1503..2759
                     /locus_tag="PK710_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10397.1"
                     /translation="MKAAASKPWLVRIVVCLVMFFSLSSASAAVRLQGQWDGHDPLVT
                     LDADQVTPGQALNLLANAAGWSIVAPDLGNRSLSLGVRDQPASSVLAMILSDTDYVAR
                     QDNDRVFITTEAVPPQEESHDSRQDRSAFGSDIRIEQGESVRNVVATGGNIDIFGHVQ
                     KNVNAFGGDVFVHENASIGGDVQVVGGTVHLADRSRIDGNVDVIGGSLERDASAVVRG
                     SISDNGTTIDATSLETKEHQASVFEPTSWMSQLVSDTQHAISRTVLLFLFGVVFWALL
                     SPQMQALEVEVAARPMRSLALGLVGLLTATLAAIVLIVTVVGIPFAAVGVITTIVVAY
                     VGACAVLSTVGQALLRHKTQNRYIHLAVGCALFLLLTAIPYMGFFVWSGVLLIGLGAV
                     VATRGMGLVPRKERYPDQGPYRTAPC"
     gene            complement(2897..3226)
                     /gene="trxA"
                     /locus_tag="PK710_11540"
     CDS             complement(2897..3226)
                     /gene="trxA"
                     /locus_tag="PK710_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOT10398.1"
                     /translation="MGLSKNVLAFTDSNFDTEVLKSDTPVLIDFTAAWCGPCKLLSPI
                     VDKLADELEGKIKVGKVDIDNSPNVTARYGIRGVPTVMVFKNGEAVGQHVGLTNRETL
                     LKLIENA"
     gene            3368..4348
                     /locus_tag="PK710_11545"
     CDS             3368..4348
                     /locus_tag="PK710_11545"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOT10399.1"
                     /translation="MTGSFRTILLVLLLGCAERPAHDRPSLLCSQPVAPLPSAADPVS
                     PPETPASAEPSPTAPLPIPEEMAGVPGGQFYVGQDNIGEMDERPRHLVTVKPFLLDRT
                     EVTHEAYYRCVEANVCRPHSSASTAANHFGDDRKFRTPTRPISGVSHEDARTYCAWVD
                     KRLPTEVEWERAARGNDERLYPWGNEEPTRDLAVFGGTITQPVGSTPRGAGPYGHLDL
                     AGNVWEWVEDHYDPYAYRRSSADVGVPGSCDEILATQDELRKTGQQGFTGSNPIPTEC
                     EFVLRGGAFNYGAKGLRSSNRVHHPGRFRLVMSGFRCARDWPTGPTESTE"
     gene            4345..5133
                     /locus_tag="PK710_11550"
     CDS             4345..5133
                     /locus_tag="PK710_11550"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HOT10400.1"
                     /translation="MNTKKPSILTAPRSAMPYSFLALSAMLTLVAGCQSKEEAVWIAC
                     DAPNKVDPSLPPAERGQALANLIGRDVKNRDVLALLESPEHPTEKANKLNELAKSYHI
                     ETCSLAALWSNVPTPTAALSQVPPPRFLPPVLEPQVLGELPMDAIQQTMQRQSSKLRA
                     CYEAGFRRDHSLAGVVLVRFVVGTEGNVIEATDAGSTLSDPQVVDCLLQEIRKTTFPK
                     PKNGNATVLFPIQLRLDATAAASASHAPASASSAPPSPPAASSP"
     gene            complement(5193..5807)
                     /locus_tag="PK710_11555"
     CDS             complement(5193..5807)
                     /locus_tag="PK710_11555"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10401.1"
                     /translation="MRTRNVWLAALVMSLAMGTVARANAQPRQRFTFEQLDKNGDGKI
                     VASEVPAWSWQRLSQADTNKDNAVTKAEFDAFRASRPGRGANWARPTFEQLDKNGDGK
                     IVASEVPAWSWQRLSQADTNKDNAVTKAEFEAFRASRPGRGANRARPTFEQLDKNGDG
                     KIVASEVPSWVWQRLSQADTNKDNAVTKAEFEARRTQRPRRAKP"
     gene            6057..6254
                     /locus_tag="PK710_11560"
     CDS             6057..6254
                     /locus_tag="PK710_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10402.1"
                     /translation="MLPSMMSTAKLPGQSPVISFDGISIPSITHVIAMVRFIHSAKIW
                     LIRDVRHSFDKRTNLSVFVSH"
     gene            6350..7663
                     /locus_tag="PK710_11565"
     CDS             6350..7663
                     /locus_tag="PK710_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10403.1"
                     /translation="MSTRWWLAASVLCSAFLGAVPAWGQAVFRGQEIYEMARSAIDIT
                     DNELRLHQANERFGTMYSHAAVTSRWAPGEFLMADGTKLGGNLARNQVLVGGGWGDTE
                     EGLFFLGAQIDQLVAVGQPGQEKQSDSERFKPGGVAQMLYYLGGSLYGVQASAGLLFN
                     QMGPEKLLLDGYGNFSARAGGSPRPGAPTDVQDPVRSQNPPNSLLFTVYDGHTGAYIA
                     MTYGGQQERTMSEDWMGNRTYRTEETGSGIQDVRVNVQPLQHYLPAWFGLPFVALTRY
                     DRPFSELTASTEEQSATKTTDFALGADDVLRGGLRWRTVINQDGRLRIAELGYVEEVR
                     NGEFGFLLGARAMAVNRTGTHEPSAEGFLKASYMRSEPDVDDKAVPSWVGVAVSYSYN
                     TPDSATFIPIPKAHVMGIQVIYGPTEVAKPLIPLVRAVEDREEDD"
     gene            7663..8874
                     /locus_tag="PK710_11570"
     CDS             7663..8874
                     /locus_tag="PK710_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10404.1"
                     /translation="MATWNRFSTIGAIIIAGGLPAACAPLEAPTNDSQGASRFVLEPL
                     LRQGSGFLCPPLELHFEFYAVGDDIDAEVSSVRQPDGSTLVFVSGTHTMTFANDTTSA
                     NFHCFGWFANHGVHSIEMPAAPPTSLPPTRLLPFTHGTLIQSWKTLWWKIGSPSAVEI
                     DIQNLSGIIGIRADRTGPMTMSDREGMVVFTRWTIDETPKANPDGDEVLFSIPAGSVL
                     GSVRYADEVAPGVFAWVQTMGESAQYFRTDRSTPIDANLSEFNIKFVERNPDTGHIVL
                     IGDAIHDWDVDGTGALITPPLPATSEGYAPWNIARRAVALSERSMPNPNAEQLRVPIS
                     YQGAFLEAETMVVHELPSTPCTDRESCRQVGEHYLLGAWQSANHRYGVYYSWPWLEDG
                     IRPLLVVAPLP"
BASE COUNT         1908 a         2832 c         2458 g         1898 t
ORIGIN      
        1 caagagcttg accgatagat gggcaaatcc gccatgacca ccccggctcc atcggcaaaa
       61 tgatccgatg ccgcaaggac ctgtccacga gcggatagca ccgcgctgat gccattggtg
      121 gccgcccgaa tgacaccgta acggttttca atcggtcgca tacggctgtt ttcgtaatga
      181 taatccttga ccgcgtgcca gtcgttcgtg ggaactagca cgatacttcg tctgtctcgc
      241 ccatatcccg acgccaagtc ctcgaagttg tcgtcttggc aaatcatggc gcccacggca
      301 tttccgcgca cctgccaatc cacacgagtg ccatcaccgg cctgatatcg ttcgatgaaa
      361 gggactcgat gggtttttcg gtattcgatt ggatgggccc ctttccgatc gatgagcacc
      421 acacgattgt cgtcatgcgt tcgatcgaaa tatccgatgg ccaacgccac atctcgttgt
      481 tgcgcgagca tgaaaagagg ctcgagcaag aggtcccgcg tctgggtcgt ggtcatgaac
      541 ggagcttccg ggcaaaccac caatgccgct ccctggtcca cagcctttcg aacgagggga
      601 atcaccatcg tttcaacgac ttgaggcgaa ggcgtgcgtg cgcccaaagg catatcgtca
      661 tcgacccacc caatcgcggc cacacgcaga tgggatatgg gtggttcgaa ccaacgggcc
      721 gcattgtata gggtgatgag tccgaacatc acggcgagag acgtcaggcc acgacgcggc
      781 gaaagggttt ttcgcaggag ccgaaccacc acggcttgcg cgctgacaag cacgaacacg
      841 atgcctgttc cgcccgtgat atccacgaat tgaatggccc aggggctctc gctccacacg
      901 cgcacgaagc tttgtgccgt cccccatatc ggaatcgcgt gcgtggctat ccattccacc
      961 aaagccacca ccgcaccgac ggcgaaagcg ccatgcaggc caggccatcg gttcacacga
     1021 taggcaatcc acgaagcgac catccaccca aacaccgtat aaatcccaag ccctatcgcc
     1081 atcatccacg gaaaggcggt cacgacgaga agaggggccg cataaaaaac accgagtatc
     1141 gctccagcac cgaggggcac aagccaggcg cttcgaaatc cttccttctt tggcccgcta
     1201 tcgaggctgc ctatccaaaa aaggaaagcg aaaagctgaa ggggccaaag atgcgcgggg
     1261 tagtccgata aaaagagcaa ggaaccactg aaggcgggca aaacgatgcg taacatctgg
     1321 tttggccggg ggattgtctt tgccaagcga ttccgtgtca tgcgtaacta cttgataata
     1381 tagaaatttt tcaaactgcc acccagtatc ttccttcggt tcgaaatttt tttctcccct
     1441 gccacgaatc ctcgcagcgg gcgccgacgc gcccttcccc ccatgacacg aaaggagcct
     1501 tcatgaaagc tgctgcctcc aaaccatggc tcgtgaggat agtcgtctgt ctcgtcatgt
     1561 tcttttctct gtcttcagct tctgccgccg tacgtttgca gggacagtgg gatggacacg
     1621 atccgctcgt cacgctcgat gccgaccaag tcaccccagg gcaggccctc aaccttctcg
     1681 cgaacgccgc gggatggagc atcgtcgctc ccgatctcgg gaaccggtcc ttgagtctcg
     1741 gcgttcggga tcaacccgcc tcctccgtcc ttgccatgat tctttcggac accgactacg
     1801 tggcccgcca agataacgac cgcgttttca tcaccaccga ggcggtcccg ccccaggaag
     1861 agagtcacga ctcgcgccaa gaccgctccg cgttcggcag cgatattcgg atcgaacaag
     1921 gtgaaagcgt tcgaaacgtt gtcgctactg gcggcaacat cgacatcttc ggccatgtcc
     1981 aaaagaacgt gaatgcgttc ggaggcgacg ttttcgtaca tgaaaacgca tccatcggag
     2041 gcgacgttca ggtcgtggga ggcacggtcc atcttgccga tcggtcccgc atcgatggta
     2101 acgtggacgt gataggagga agtttggagc gagatgcgag cgcggttgtt cgtggcagca
     2161 tctccgacaa cggcacgacc atcgacgcca cgagtctgga aacgaaagag catcaagcaa
     2221 gcgtattcga gcccacaagt tggatgagcc agctcgtcag cgatacgcaa catgccatct
     2281 ctcgaacggt gttactgttc ttgttcggtg ttgtattctg ggctttgctc tcgccgcaaa
     2341 tgcaagcgct tgaagtcgag gtagcggcgc gccccatgcg ctcgctcgct ctgggactcg
     2401 tgggcctgct tactgccacc ctggccgcca tcgttctcat cgttaccgtc gtgggaatcc
     2461 ccttcgccgc cgtcggcgtc atcaccacga tcgtcgttgc ctatgtcggc gcttgtgctg
     2521 tgctcagcac ggtcggccag gcgttgttgc ggcacaaaac ccagaaccga tacatccatc
     2581 tcgccgtcgg atgcgcgcta tttctgctgt tgacggcgat tccctacatg ggctttttcg
     2641 tatggtcggg tgtgttgctc atcggtctgg gtgcggtcgt ggctacccga ggcatggggc
     2701 tcgtgccaag aaaagaacga tatccggacc agggcccgta tcgaacggct ccttgttgaa
     2761 ccgcccaaca ctggacacgc ctcaccctac aggagcctcg aaaccacgag gctctttcgc
     2821 ttatggaccc caccgcggcc cgaagcatgg cagctacagc atcggcgatg ccccgagcga
     2881 tatcaatcgc acccgatcag gcgttttcaa tgagtttgag caaggtctct cgattcgtca
     2941 ggcccacgtg ctgacccacc gcctcgccat ttttgaacac catcaccgta ggaactccac
     3001 gaattccata ccgtgccgtg acattcggcg agttgtcgat atccaccttg cccaccttga
     3061 tcttgccctc gagttcgtct gcaagcttgt ccacgatggg agacaacagt ttgcaaggac
     3121 cacaccatgc cgccgtaaag tcgatcagca ccggagtatc gcttttcaac acctccgtat
     3181 cgaaattcga atccgtgaag gcaagaacat tcttcgacaa tcccatgatt ctctccgtga
     3241 ccaggtcccc aaagacctct ttccaaacgt tgaaacaagc ccttggatgc cgcgacccta
     3301 ggagccggtt gcgctggggt caaggatcgg gcatcggaag atccatctgc tcacccccac
     3361 acgcatcatg accggatcct ttcgaacgat cttgcttgta ttgttgctcg gttgcgccga
     3421 gcgccccgcc cacgaccgcc cttccctcct ctgctcacag cccgtcgcgc ccctgccgtc
     3481 cgctgccgac ccggtttctc cccccgagac accagcaagc gcggaaccat cccccaccgc
     3541 acccttgccg atcccagaag agatggcggg cgtaccggga gggcagtttt acgttggaca
     3601 agacaacatc ggcgaaatgg acgaacgccc tcggcacctg gtcaccgtca aaccgttttt
     3661 gctcgaccgc accgaagtaa cccatgaggc ttattaccgt tgcgtcgaag ccaatgtctg
     3721 tcgccctcac tcctctgcat cgacggcagc caaccatttc ggcgatgatc gaaagtttcg
     3781 cactcccaca cgtcccatca gcggcgtgtc ccacgaggat gccagaacct attgtgcatg
     3841 ggtggacaag cgccttccca ccgaagtcga atgggagcgc gctgcaagag ggaacgacga
     3901 gcgcctgtat ccctggggga atgaagaacc cacccgagat ctggccgtgt tcggaggcac
     3961 catcacgcaa cctgtgggca gcacccctcg aggcgctggc ccctacggtc acctcgacct
     4021 tgccggcaat gtctgggaat gggtcgaaga tcattacgac ccgtatgctt atcgaagatc
     4081 atccgccgac gtcggagtac ccggttcctg cgatgagatc cttgccacgc aggatgaatt
     4141 acgaaaaacg ggccaacaag gctttacagg tagcaatccc atccccacgg aatgtgagtt
     4201 cgtccttcga ggtggcgctt tcaactacgg cgcgaaagga ctgcgaagct ccaatcgagt
     4261 ccatcacccc ggacgatttc gccttgtgat gagcggattc cgctgtgcca gggattggcc
     4321 caccggtccc acggagagta ccgaatgaac acaaaaaagc catcgatcct gaccgctccc
     4381 cgcagcgcca tgccttactc atttcttgcg ctgtcggcca tgctcacgct cgtcgccggc
     4441 tgtcagtcca aagaagaagc cgtatggatc gcctgtgacg ctcccaacaa ggtcgatccg
     4501 tctctacctc ctgcagaacg gggacaggcc ttggcgaacc tgatcgggcg cgacgtcaaa
     4561 aatcgcgacg tgctcgcgct gctggaaagc cctgagcatc caactgaaaa agctaataaa
     4621 ctcaatgaat tagccaagtc ttaccatatt gaaacctgct cgcttgccgc cttgtggagc
     4681 aacgtgccaa cccctacggc ggctttgtcc caggtcccgc ctccacgctt tctcccgccg
     4741 gttctcgaac cccaagtgct gggtgaatta cccatggacg ccatccagca gaccatgcag
     4801 agacaatcgt caaaactccg cgcctgttac gaagctggct ttcgccgcga tcacagcctc
     4861 gcgggcgtgg tgctggtgcg tttcgtggtg ggaacagaag gtaacgtcat cgaagccacg
     4921 gatgcgggaa gcaccctttc cgatccacaa gtcgtcgatt gcttgctcca ggagattcgc
     4981 aaaacgacgt ttccaaaacc caaaaacggt aacgcaaccg tgctatttcc cattcaattg
     5041 cggcttgatg cgacggctgc cgcttcggct tcgcacgctc cggccagcgc aagttccgcc
     5101 cctccttccc cgcccgcggc gtcgagcccg tgaatcaaac gagcgaaaac gcggaggatg
     5161 cgtggttggt cgcgagggcc ttgtcatagg gttcagggct tggcgcgacg tggccgctgc
     5221 gttcgtcgag cttcgaattc cgctttggtt acggcgttgt ccttgttggt atccgcctgg
     5281 ctcaaccgct gccataccca ggaaggcact tccgaagcca cgatcttgcc atcgccattt
     5341 ttatccaatt gttcgaaggt gggacgcgcc cgattggctc ctcgacctgg ccgtgacgcg
     5401 cgaaacgcct cgaattccgc tttggtcacg gcattatcct tgttggtatc cgcctggctc
     5461 aatcgctgcc agctccaggc ggggacttcc gaggccacga tcttgccatc gccattttta
     5521 tccaattgct cgaaggtggg acgcgcccaa ttggctcctc gacctggccg tgacgcgcga
     5581 aacgcgtcga attccgcttt ggtcacggca ttatccttgt tggtatccgc ctggctcaat
     5641 cgctgccagc tccaggcggg aacttccgag gccacgatct tgccatcgcc atttttatcc
     5701 aactgctcga acgtgaatcg ctgccgaggc tgcgcgttgg ctcgagccac ggttcccatc
     5761 gccaaactca tgaccaaagc tgccagccat acattcctag ttctcattgg gcatcatctc
     5821 cttgccacga atgtgttcat tggtgcttac aacggagcac aggcacaaac ccgtcgcagg
     5881 attttttccg gcccaaatta cgaagtgcaa aacgactact tggacgtggc tcccccctaa
     5941 cgtacttcga tgtccatggc ctgggaccag tccaaaacaa tgtgtgcgag aagaccaccg
     6001 cactcgtctc gttctctatc cggatacgta tagatggctg cactgctcga aaagccatgc
     6061 ttccgtccat gatgtcgacc gcgaagctgc ctggacaaag tccagtgatt tcgttcgacg
     6121 ggatatcgat accttcgatc actcacgtca tcgcgatggt tcgattcatc cattcagcca
     6181 aaatctggct tatccgcgat gtgcgccatt cgttcgacaa gcgcacgaat ctatccgttt
     6241 tcgtttccca ttgacgcact gccgggtgat agtagtagcg ttgacagtgg tgcaatacct
     6301 tcaccgcttc cctttcacac cagcttccca tcccctgcag ggcagggccg tgagcacacg
     6361 gtggtggctg gcagcatccg tgctttgttc tgcttttctc ggcgctgttc ccgcgtgggg
     6421 acaggcggtc tttcggggtc aagagatcta cgaaatggcg aggagcgcca ttgacatcac
     6481 cgacaatgaa ttgcgtctgc atcaggccaa tgagcgattc ggcacgatgt attcgcacgc
     6541 tgcggtgacc agccggtggg ctccgggtga attcttgatg gccgatggca cgaagcttgg
     6601 aggaaacctg gcgcgaaatc aggtgttggt gggaggcggt tggggtgaca ccgaggaagg
     6661 attgtttttc ctgggagcgc agatcgacca gttggttgcc gtgggccagc ctggtcagga
     6721 aaaacagtcc gactccgaac gattcaagcc cggtggtgtc gcccagatgc tgtactacct
     6781 cggtggatcg ctgtatggcg tgcaagcctc tgcgggactc cttttcaacc aaatggggcc
     6841 tgagaagctt ctgctcgacg gatatggcaa cttttccgcc cgagccggtg gtagccctcg
     6901 gccgggtgcc cccaccgacg tccaggatcc ggtccgctca cagaatccgc cgaattcttt
     6961 gctatttacg gtctacgatg ggcacacggg cgcctatatc gccatgacct atgggggcca
     7021 gcaagagcgc accatgtccg aagactggat gggcaatcgg acctatcgaa ccgaggaaac
     7081 gggatctgga atccaagacg tacgggtcaa cgtacagccc ttgcagcact acttgcccgc
     7141 ttggttcgga ctgccgttcg tagccctcac ccgctacgac cgtccttttt ccgaactcac
     7201 tgcttccacg gaggagcagt cggcaacaaa aaccactgat ttcgcgctcg gtgccgatga
     7261 cgtgctgcga ggcggattgc gatggcggac cgtcatcaac caggatggac gccttcgaat
     7321 tgctgaactc ggttatgtcg aagaagtgcg aaacggtgaa ttcgggttcc tgctcggagc
     7381 acgtgccatg gccgtcaacc gcacgggtac ccacgagccg tccgcggagg gattcctgaa
     7441 agccagctac atgcgctcgg agcccgacgt agacgacaaa gccgtaccga gctgggtcgg
     7501 cgtcgcggtt tcctacagct acaacacgcc agatagcgca accttcatcc cgattcccaa
     7561 ggcccacgtc atgggaatcc aggtcatcta cggccctacg gaggtcgcca aaccgttgat
     7621 tccactagtc cgcgccgtcg aagaccgcga ggaggacgac taatggcaac ctggaatcga
     7681 ttctccacca tcggggccat catcatcgca gggggcttgc ctgcggcttg tgctcccctc
     7741 gaggctccca ccaacgattc ccaaggggca agtcggttcg tcctcgaacc gttgcttcgg
     7801 caaggctccg ggtttctatg cccacctctc gagctgcact ttgagttcta cgcagtcggc
     7861 gatgatatcg acgcagaggt ttcatcggtt cgccaaccag acggcagcac gctcgttttc
     7921 gtgtctggca cgcatacgat gacgtttgcc aacgacacga cctctgcaaa tttccactgt
     7981 ttcggatggt tcgccaacca cggggtacat tcgatcgaga tgcccgcggc accgccaacc
     8041 tcgctgcccc ccacacggct gctgcccttc acccacggta ccttgatcca aagctggaaa
     8101 accttgtggt ggaaaatcgg ctcccccagc gccgtcgaaa tcgatatcca aaacctttcc
     8161 ggaatcatcg gcattcgggc cgacaggacg ggaccgatga ccatgtccga tcgggaaggt
     8221 atggtagtgt ttacgcgctg gaccatcgat gaaaccccca aggccaatcc ggatggtgac
     8281 gaggtccttt tttctatccc cgccggctct gtgctgggat ccgttcgata tgccgatgaa
     8341 gtcgcgcccg gggtgtttgc gtgggtacaa accatgggcg aatcggccca gtacttccgc
     8401 acggatcgtt ccaccccgat agatgctaac ctatcggaat tcaatataaa attcgttgaa
     8461 cggaacccag atacggggca tatcgttctc atcggcgatg cgatccatga ttgggacgtg
     8521 gatggaacgg gcgcgttgat aacgccccca ttgccagcca cgtccgaagg gtatgcaccg
     8581 tggaacatcg cccgccgtgc ggtggctttg tcggagcggt ccatgcccaa tccgaatgcc
     8641 gagcagcttc gggttccgat cagctatcag ggcgcctttc tcgaagccga aacgatggtt
     8701 gtccacgagc taccctcgac gccttgtacc gatcgggaat cctgtcggca agttggcgag
     8761 cactatctgc tgggggcatg gcaatcggcc aaccatcgct atggcgtgta ctactcttgg
     8821 ccgtggctcg aagacggtat ccggcctttg ctcgtggtgg cgccgctgcc gtagtagggg
     8881 gtgggcactc tgaacagaga attcaacgtt attctgccgt gaaaagggta agctccccac
     8941 ccaatgcagt tccgccagat ctccccgtcc gccattttgc ttagcgttgg cttcatcgct
     9001 tgcacctcga acgagacggc cgtcaccaac ccggccatgg aaaagcccgt gcagcccacg
     9061 cccagcgcat cgccgccccc aagcgccacc catgcc
//
LOCUS       DAOSOR010000187         9096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_7601_length_9096_cov_4.179958, whole genome shotgun sequence.
ACCESSION   DAOSOR010000187 DAOSOR010000000
VERSION     DAOSOR010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9096
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7601_length_9096_cov_4.179958"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PK710_11575"
     CDS             <1..515
                     /locus_tag="PK710_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10405.1"
                     /translation="PNIVNRIYWPVGDPSRKRAYEWGERARVGHRYHDRILLVQGPLA
                     LVRRPGSLRIRIEASSLTAKDPGTPARIRSWLDQHIHVQGRPEWVFVKVHTHGAPEHQ
                     AESLLGKGGYEMHRELNARYNDGVRYSLHYVTAREMVNIAWAAMDGKTGNPNAYRDYE
                     LAPPPLVRRA"
     gene            complement(572..1165)
                     /locus_tag="PK710_11580"
     CDS             complement(572..1165)
                     /locus_tag="PK710_11580"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOT10406.1"
                     /translation="MRNLLVLTTLVTSLAACSPPPSNFPYEREPDPRRSEYVIGISDR
                     LSIRVWKNPELNTDIEVRPDGTITMPLLGDVRAVNLTPTELKKEIGKALAQYVKDAGA
                     VVTVAVTEVNSYKITVSGNVIQPGVYPSRQFLTVADAVALAGGPNRFAKVNEVILIRP
                     NPKGGVRRIPIDYEAVQKGESMQQNLVLLRGDTLFFP"
     gene            1216..2586
                     /locus_tag="PK710_11585"
     CDS             1216..2586
                     /locus_tag="PK710_11585"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOT10407.1"
                     /translation="MFILPGILGLVFLIFVRPFEFIEALQGIPMLYMAFGLAVFGYAV
                     DVRLGFSDVRPVPQLVWSGIFFVWCVITGLVKAPGELAGEALRLTVALILFYVVAHGV
                     RTFKMFEILASAVLVCSLFVCAVCVHQGLSERQCIALAPHEGRLSTGVADGRFCEEPE
                     QCYGPQAEPGYSYRCEKAGLLGTSAIGDRVRYLGLLQDPNEVSLVVAAALPIAFAFYQ
                     RRKTSGRLVLLIVSLLLVALTVLFSKSRGGQLVFLTVIGVYFIKKYGWKGAMLGALGA
                     LPILAFGGRGGEEASESSLERLECWYAGVSMFRADPIFGVGLGQFTQHHFLTAHNSYV
                     LAAGELGLVGTVVWVSIIWLSVKIPWVAMVDLENVPGADVARIWSVALLASMAGMAIG
                     VFFLSFNYHYILWLFVGLSGSFFMAVKGHLPEWRVPFGWKDITAVTLGSLWVIVVIFL
                     YTRGKI"
     gene            complement(2589..3323)
                     /locus_tag="PK710_11590"
     CDS             complement(2589..3323)
                     /locus_tag="PK710_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOT10408.1"
                     /translation="MPPRSVLITKRLIDVVAATIGLSMTLPLYPAIAAAIKLDSKGPV
                     FFKQTRAGRLLPRDPEDNDPTPRCETFTLVKFRTMGIDAEKKTGAVLAQKNDPRVTRI
                     GKFLRKTRLDELPQLWNVLRGDMSLVGPRPERPELLVNFALAIPFFEERMRDVKPGLT
                     GMAQVSLGYTGAIPDDSVLAAFRDTLQNPFELEEAEGALADDLRAKLLFDVAYTAALE
                     DFRSFLETELSVILKTPLIMIKSSGH"
     gene            3488..4042
                     /locus_tag="PK710_11595"
     CDS             3488..4042
                     /locus_tag="PK710_11595"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT10409.1"
                     /translation="MYHSLQWALPIVFSSTGGKPSRLWVERHWLRTGEFVILLRVPGT
                     LEYRELATRVVTTACKLIRESEQGKTSGLSEEFTYEAVSAVGEAFNNVALHGYENLPT
                     GDIIFEVSMFEDRLVIDVKDFGHAFHPDNAPEPDLGELPESGMGLFIIRAFVDELTYE
                     PGSPNCLRMVKRFGVRKNETRKVE"
     gene            4125..4427
                     /locus_tag="PK710_11600"
     CDS             4125..4427
                     /locus_tag="PK710_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT10410.1"
                     /translation="MMFSKTVEGDAIRIEISGQLDSMTAPELRPSFEALLQENPKRIV
                     LDLSGLRLIDSSGVGAIVSLFKQVRAAGGAFEVVGVQGQPRSIFQVLRLDRVFNIA"
     gene            4605..5663
                     /locus_tag="PK710_11605"
     CDS             4605..5663
                     /locus_tag="PK710_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10411.1"
                     /translation="MSDDWTDDEFGWREVRAELARIRRRARARWLSIGVISLVLTALV
                     VAKQARSERTFRSTVTLRVIEGQFDPDSAPPTSAQLQDYLWEVALSRSRLLEVMEAHD
                     LYPSLRKIDPLIALDEMRDVLDVKVLRNYFQVERDPDAPPRSARIALSYSHASPEKSI
                     EVARALANLMAEQEAKSRRATAEVALQSVKTSASALDDALVAARSELAQVTYQMSTAP
                     RDEAAGLAVRAIGLRKRILSLEEQKDLASSAVSSLGMQTEVEGKELGLRFEVIDPGRV
                     PRVVVSSSTRLIFIGFFTFLLLLPIASTAVAAYDLRVYDLEDLRRLGLEPFGHIPSFP
                     GYRQATLNDRLRQRTGAS"
     gene            5660..7168
                     /locus_tag="PK710_11610"
     CDS             5660..7168
                     /locus_tag="PK710_11610"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOT10412.1"
                     /translation="MIEKNTSKAMLAPRDVIHVALSILRRSARRWPVVALATVIGLIA
                     AFVLPKLMPPVFQSETLILYQETIQTENLLGAQQYAMESRRQIGMRLREMLLSRTNLE
                     QIIKAHNLYPSTVSSRGMIDAVEEFRAKIDCRVRENETFRIVYQGEDPDVVYEVTKAL
                     SESLVEQNRQYRTEQAQATKQFLEAEQKRMAADLSQKEQKLAMFLADHPEFAQDAATS
                     ASAAGASVRAAARSSATVDTGLDALERQARRLRSQLASPDPPPASAFMGPSIDPKAAE
                     AIAAAESALADARRDLQDKQSKYTQQHPDVVEAQNKVRAALARLNQAKAAARPASAPM
                     TETPSSPEERREELKTQLARVEAAIAAAKRGQSAAVPEDNSGESNRVVTLETQWSSLN
                     REVNETREQHEQIQTRLFRATMVATVEASGQASQMVVVDDAYKPKRPSRRGAKRTGAI
                     AAFVVLMVGATIALGLGLLDDRVYEERDLKKLALGPIVHVVPSPVRKGKKYG"
     gene            7161..8141
                     /locus_tag="PK710_11615"
     CDS             7161..8141
                     /locus_tag="PK710_11615"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HOT10413.1"
                     /translation="MADQPQGRPPQNSYAQAPGGWDQNAQRESPIARRPAGWRPPRDP
                     AQAALEATIVGSLNVADDEPTRLGQSLDTAGTVERAAAPKGAAAPAAQQRPPSEPPRA
                     ESAVRAGYVQVVATVVDPPPGTHNLPFIKDADSPVAASYRVLRHRLREANNPSTIAVT
                     SPGRSEGKTTCAINLAMALAEHGREKVLLVEANLRFPHLAAALGFTAPSCFGRQMAAH
                     LKSPLSPWEVVAAFFHNLHVLAVDPSSVGQWRMSAPGLKLAMDQLQASGYKHIIVDCP
                     SALGSADVNVIEDTTDGVLLTARPGKTTAESLRKTERHLAPANILGVVLT"
     gene            complement(8194..8694)
                     /locus_tag="PK710_11620"
     CDS             complement(8194..8694)
                     /locus_tag="PK710_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10414.1"
                     /translation="MGDDKDDYKQRVEREARMGRKFSLADAVGREGGGFFRGTSPFPR
                     LQQVQSELSHFVSDHVRDASGALRAVLIRHIMTNEPLISQHFEDPLAALEKILDRLLD
                     EGMYYDFVKEVDMEWGQLMLERPYFQEPGEPAHEHDEYTHESVRVELMMLREIARGAR
                     EEPSES"
BASE COUNT         1874 a         2307 c         2786 g         2129 t
ORIGIN      
        1 agcccaatat cgtgaatcga atctattggc cggtgggaga cccatctcgc aagcgggcgt
       61 atgaatgggg cgaaagggcg cgtgtggggc atcgatatca cgatcggatc ttgttagtgc
      121 aaggcccttt ggcgttggtg cgtcgtccgg gctcgttgcg cattcgcatc gaagccagtt
      181 ctcttacggc aaaagacccg gggacacccg cgcgcattcg ttcatggctc gatcagcaca
      241 ttcatgtgca ggggcgcccg gaatgggttt tcgtcaaggt tcatacgcat ggggcacctg
      301 agcatcaggc cgagtccttg cttggcaagg gggggtatga gatgcaccgt gagctgaacg
      361 cacgctataa cgatggggtt cgttacagtc ttcattacgt cacggcccgc gagatggtga
      421 atattgcgtg ggcggcgatg gatgggaaaa cgggaaatcc caatgcatat cgggattacg
      481 aattggctcc gcctcctttg gttcgacgag catgaataca tgtaaacgct ggaaacgatt
      541 ggatttttcg caagagcgat ggtgggatga ttcagggaaa gaagagggta tcaccgcgca
      601 gcagtacgag attttgttgc atcgattcgc ctttttgaac ggcttcgtaa tcgatgggga
      661 tacgtcgaac gccgcctttg ggattggggc gaatcaggat cacttcgttg actttggcga
      721 aacggttggg gccaccggcg agagcgacgg catcagccac cgtaagaaat tgtcgcgagg
      781 ggtaaacgcc gggttggatg acgttgccgc tgacggtgat tttgtaggag ttgacttcgg
      841 tgacggcgac ggtaacgaca gcgccggcat ctttgacata ttgagcgagg gcttttccga
      901 tttctttttt caattcggta ggagtgaggt tgacggcacg cacatctccg agcaagggca
      961 tggtgatggt gccatcgggt cgaacttcga tatcggtgtt gagttctggg tttttccata
     1021 cgcgaatgct gagtcggtcg gatatcccga tgacgtattc gcttcgtcgt ggatcgggtt
     1081 ctctttcgta ggggaaattg gacggaggcg ggctgcaagc cgccaacgat gtgaccaggg
     1141 ttgtaagaac gagtagatta cgcatgacca tcgcgcagaa ggtctaggcc acctgcgcct
     1201 cggatggaag gaccgatgtt cattctgcca ggaattctcg gacttgtgtt cctgatcttt
     1261 gtgaggccgt tcgagtttat cgaggcactg caagggatcc cgatgctgta catggcgttt
     1321 gggcttgcgg tgtttgggta tgccgtcgat gtaaggcttg gtttctcgga cgtgcggcct
     1381 gtgcctcagc tagtgtggtc cgggatattc tttgtctggt gcgtcatcac ggggctggtg
     1441 aaagcgccgg gggagttagc cggtgaagcg ctgcggctta cggtggcact gatcttattt
     1501 tacgtggtgg cgcacggtgt gcgtaccttc aagatgttcg agatattggc atcggcggtg
     1561 ctggtgtgtt cgctgttcgt ttgtgcggta tgtgtgcatc aagggctttc ggaaaggcaa
     1621 tgtatcgcgc tggcacctca tgagggaagg ctgtccacgg gggtagcgga cggacggttt
     1681 tgcgaagaac cggagcagtg ttacggacct caagcggagc cagggtattc ctatcgttgc
     1741 gaaaaggcag gattgttggg cacctcagcg attggggatc gcgttcgcta ccttggattg
     1801 ctccaagatc ccaacgaagt atctcttgtc gtggctgccg ccctgcccat tgcgtttgcg
     1861 ttttatcagc gtcgcaagac gtcggggcgg ctcgtgttgt tgatcgtatc gctgcttttg
     1921 gttgcgttga cggttctatt ttccaagtct cgtggtgggc aacttgtatt tctgacggtg
     1981 attggcgtct atttcatcaa gaaatatggg tggaaggggg cgatgctcgg cgcgttgggg
     2041 gcgcttccca ttttggcgtt tggagggcgt gggggagagg aggcgagcga gtcttcgctc
     2101 gagcgactcg agtgttggta tgcgggggtc agcatgtttc gcgcggatcc gatttttggc
     2161 gtgggattgg gccaattcac ccagcaccat ttcctgacgg cgcataattc ttatgtactt
     2221 gccgcggggg aacttggttt ggtggggacg gtggtgtggg tcagcatcat ctggctgtcg
     2281 gtcaaaattc cttgggtagc catggtcgat ctcgaaaacg tgccgggggc tgatgtcgct
     2341 cgcatttggt cggtggcgct gctggcctcg atggcgggca tggcgattgg cgtttttttc
     2401 ctgtccttca actaccacta catcttgtgg cttttcgtgg gactttccgg atcgtttttc
     2461 atggctgtca aagggcatct acccgaatgg cgtgtcccct ttgggtggaa ggacatcacg
     2521 gcagtgaccc tgggaagttt gtgggtcatc gttgtgatct ttttgtacac ccggggtaag
     2581 atctgaactc aatgtcctga cgatttgatc atgatgaggg gggttttgag aatgacggaa
     2641 agctccgttt ccaaaaagct gcgaaagtct tccagagcgg cggtgtaggc gacatcgaac
     2701 agaagtttgg cacgaagatc gtcggcgaga gcgccttctg cctcttcgag ttcgaatggg
     2761 ttttgtaagg tatcacgaaa cgcagccagc acactgtcgt cgggaatggc acccgtatag
     2821 ccgagtgaca cctgggccat tcctgtaagg ccaggtttga cgtcgcgcat acgttcttcg
     2881 aaaaagggga tggcgagtgc gaaattgaca aggagttcgg gtcgttcggg gcggggcccc
     2941 acgaggctca tgtctccgcg caggacattc cagagttggg gcagttcatc gagtcgagtt
     3001 tttctaagga atttgccgat tcgtgtgacg cggggatcat ttttttgtgc gagcaccgcc
     3061 cctgtctttt tttccgcatc gatgcccatc gttcgaaatt tcacgagagt gaaggtttcg
     3121 catcgaggtg tggggtcatt gtcttccggg tctctgggaa gcagacgtcc ggctcgagtc
     3181 tgtttgaaaa aaacgggtcc tttggaatcg agtttgatgg ctgcggcgat ggcgggatac
     3241 agcggaagcg tcatgctgag cccgatggtt gcagccacca catcgatgag tcgtttggtg
     3301 atcagaacgg aacgaggggg catcgaaatg ttcctgtggg actgtgtttg tcggcaagtg
     3361 aggtgaagtt actgtacctt tgactgggcc cagggtggta tcgtaccctt gtgtatcgga
     3421 aggcaacagt ctttcccgca gtgtataacg ttggttgtac gagacggcga ggggttcgac
     3481 ccatggtgtg tatcattccc tgcagtgggc tttgcctatc gtgttttcat cgactggggg
     3541 caagccttcg cggctttggg tcgaacgtca ttggctccgc acgggggaat tcgtgatttt
     3601 gttgagagtt cctggaacgc tagagtaccg tgaattggcc actcgtgtcg tgacgacggc
     3661 atgcaagctc attcgggaat ccgagcaagg aaaaacgagc gggttgtcag aagagttcac
     3721 gtacgaagcc gtttcggcag ttggcgaggc tttcaataac gtggctcttc atgggtacga
     3781 aaacttgccg acgggggata tcatttttga ggtttcgatg ttcgaggacc gtttggtgat
     3841 cgatgtcaag gattttgggc atgcttttca tccggacaat gcaccggaac ctgatttggg
     3901 tgaacttcca gaatcgggga tggggttatt catcatccgc gctttcgtgg atgaactcac
     3961 gtatgaaccg gggagtccga actgtttgcg catggtgaag cgatttggcg ttcgaaaaaa
     4021 tgagactcga aaggtggaat gacgatgcgg acggccctag acaatgacaa tgacaatggg
     4081 aagagggcgt tagcgtttcc ttccccctgt ggagtgaatg gatcatgatg ttcagtaaga
     4141 ctgtggaagg cgatgccatt cgcatcgaga tttcgggaca actcgattcg atgacagcgc
     4201 cagaattacg acccagcttc gaggctctcc ttcaggagaa tccgaaacgg attgtcctgg
     4261 atttgtcggg gcttcggctc atcgattcct ctggggtcgg tgcgattgtt tcgctattca
     4321 agcaagttcg agccgcgggg ggagcgtttg aagtcgtcgg tgttcaaggg cagccacgat
     4381 ccatcttcca ggtattgaga ctcgatcgcg ttttcaatat cgcctgacgc ctgcgtgtcc
     4441 cgaatacgtt tttccagatt ttggaaaatc caccaatggg ctccactcat ggtgagcggt
     4501 gcttggttcc ggtcaagggc cgtgggacga tgctgggcgt tggcatattg ccttggtcag
     4561 ttttaggaac gtacgaggta tggtgagaag aacgcgaacg acccatgagt gacgattgga
     4621 ccgatgatga atttggatgg agggaagtac gagccgagtt ggcgcgtatc cgcaggcgtg
     4681 cgcgtgcgcg gtggctttcc attggtgtga tttcactcgt acttacggct cttgtggtgg
     4741 ccaagcaggc gaggagcgaa cggacatttc ggtctacggt tacccttcga gtgatcgagg
     4801 ggcaattcga tcccgactcc gcgccgccca ccagcgcgca gcttcaagac tatttgtggg
     4861 aggtggcgct gagtcgcagc cggttgctcg aggtgatgga agcgcatgat ttgtacccga
     4921 gccttcgaaa aatcgatccc ttgatcgcct tggatgagat gcgtgatgtg ctcgatgtca
     4981 aagtgctccg aaattatttt caggtggagc gagacccgga tgctccgcct cgttccgccc
     5041 gtattgcctt gagttattct cacgcgagtc cagaaaaatc gatcgaggtt gcgcgtgctt
     5101 tggcgaacct gatggcggag caggaagcca agtcccgtcg tgcgaccgcg gaagtcgctt
     5161 tgcaatccgt gaaaaccagc gcttcggcgt tggatgacgc gctggtggct gcgcgttcgg
     5221 aattggccca agtgacctac cagatgagca cggcgcctcg agacgaagcg gctggtcttg
     5281 ccgtgcgagc catcggtttg cgcaaacgca tcctcagttt ggaagagcaa aaagacctcg
     5341 cgagttcagc cgtttccagt cttggcatgc aaacggaggt ggaaggcaag gaactgggac
     5401 ttcgattcga agtcatcgat ccaggacgtg tcccgcgggt ggtggtatca agctccaccc
     5461 gtctcatctt catcggattt tttacctttt tgttgcttct ccccattgca agcacggcag
     5521 tggctgccta cgacttgagg gtttacgatt tggaagatct ccgacgactt ggacttgaac
     5581 ctttcggtca tatcccttcg tttcctggtt atcgccaggc aacgctcaac gaccggcttc
     5641 gacagcgaac aggtgcatcg tgattgagaa aaacactagc aaggcgatgc tcgcccctcg
     5701 cgacgtgatc catgttgcgc tttccattct gcgccgttcc gctcgacgtt ggcctgtcgt
     5761 cgcgctcgcg acggtcatcg gattgattgc ggcgtttgtc ttgccgaaac tcatgcctcc
     5821 cgtctttcag tcggaaacac tgattcttta tcaggagacg attcagacgg aaaacctact
     5881 tggtgctcag cagtacgcga tggagtctcg acgtcagatc gggatgcgat tgcgtgagat
     5941 gttgttatcc cgaacgaatc ttgagcagat catcaaggct cataacttgt atccgagtac
     6001 ggtaagtagt cgtgggatga tcgatgcggt cgaggagttt cgcgcgaaga tcgattgtcg
     6061 cgttcgggaa aacgaaacgt ttcggattgt gtaccagggc gaggatccag acgtcgtgta
     6121 tgaggtgacc aaggcgttgt cggaatcgct cgtggaacag aatcgacaat accgaaccga
     6181 gcaagcgcaa gccacgaagc aatttttgga ggcggagcag aagcgtatgg ccgcggacct
     6241 ttctcaaaag gagcagaaac tcgcgatgtt tctggcggac catccggagt ttgcccagga
     6301 tgcagccaca tcggccagcg ccgccggagc atcggtacgc gccgccgcgc ggtccagcgc
     6361 caccgtggat acggggctcg atgcgctcga acgccaagca cgtcgccttc gttcgcaact
     6421 tgcatcaccc gatccacccc cagcttccgc cttcatggga ccgtccatcg atccgaaagc
     6481 agccgaggcg atcgctgctg ctgaatcggc gttggccgac gctcgaagag atcttcagga
     6541 caaacaatcg aagtacacgc aacaacatcc tgatgtcgtc gaggcgcaaa acaaagtgcg
     6601 cgctgccctt gctcggctca accaagccaa agcagccgca cgcccagctt ccgctcccat
     6661 gacggaaacc ccttccagtc ccgaggagcg tcgggaagaa ctcaagactc agctcgcacg
     6721 ggtcgaggcc gcgatcgccg cggccaaacg cggacaatcc gccgcggtgc cggaagataa
     6781 ttcgggcgaa agcaatcgcg tggtcaccct cgaaacgcaa tggtccagct tgaaccggga
     6841 agtcaacgaa acacgcgagc agcacgaaca aatccagact cgattgttcc gagcgaccat
     6901 ggtggcaacc gtggaagctt ccgggcaagc cagtcaaatg gtcgtggtcg atgacgcgta
     6961 caaacccaag cgtccatcac ggaggggggc caaacgcacc ggtgctatcg cggcattcgt
     7021 ggtgctcatg gtgggagcta ctatcgcctt ggggcttggc cttctcgacg accgtgttta
     7081 cgaagagcgc gatctgaaaa agctcgcctt gggccctatt gtgcatgtgg ttccgagccc
     7141 tgttcgaaag gggaaaaaat atggctgatc aaccccaagg ccgaccgcca caaaacagct
     7201 acgcccaagc cccgggagga tgggatcaga acgcgcaacg agaatctccc attgctcgcc
     7261 ggcctgctgg gtggaggccg ccgagagatc cggcccaagc tgccttggaa gcgacgatcg
     7321 ttggctccct gaatgttgcg gacgatgaac cgacgaggtt gggccaatcc ctcgatactg
     7381 ccggaacggt agagcgggcg gcagcaccca agggggctgc ggctccggct gcacagcaaa
     7441 ggccacccag tgaaccacca cgggcggaaa gcgctgtgcg cgcagggtat gtgcaggtgg
     7501 tggctacggt cgtcgaccct cccccaggca cgcataacct acctttcatc aaagatgcgg
     7561 attcgcccgt cgccgctagc tatcgtgttt tgcgtcatcg tttgcgcgag gccaacaacc
     7621 cttctaccat cgcggtgacg agccctggac gctcggaagg caagacgacc tgcgcgatca
     7681 atcttgccat ggcgctggcg gagcatggtc gggagaaggt gcttttggtc gaggccaacc
     7741 ttcgcttccc tcatttggcg gcagcccttg gattcaccgc cccttcttgt ttcggtcgtc
     7801 aaatggccgc ccatctgaaa agccccttgt ccccttggga agtcgtcgct gccttcttcc
     7861 ataacctaca cgttcttgcg gtagacccat cgtccgtcgg ccagtggaga atgagcgctc
     7921 caggactcaa gctcgccatg gatcagctcc aagcctccgg atataaacat attattgtcg
     7981 attgcccctc tgccctcggc tccgccgatg tcaacgtgat cgaagatacg acagatggtg
     8041 tgcttctcac cgcgcgcccg ggaaaaacca ccgccgagag ccttcgaaaa acagagcgcc
     8101 accttgctcc agccaatatt cttggtgtcg tgctcacatg aataatgagt ctcatgcgta
     8161 acgaggggcg tcgttccctt ccataaaaga gcgctacgac tcgcttggct cttcgcgggc
     8221 tccccgggcg atctctcgca gcatcatcaa ttccacgcga acggattcgt gcgtgtactc
     8281 gtcgtgctca tgggctggtt ccccaggctc ttgaaaatac gggcgctcga gcatcaattg
     8341 cccccactcc atatccactt cttttacgaa atcgtaatac attccttcgt cgagcaatcg
     8401 gtcgaggatc ttctccaaag ctgccagggg atcttcgaag tgctgggaaa tgagaggttc
     8461 gttggtcatg atatggcgga tcaatacggc gcggagtgcc ccgctggcgt cccgaacatg
     8521 gtcggagacg aaatgagata gttcggattg cacctgctgc agccttggga aaggcgaagt
     8581 cccacggaaa aaaccaccac cctcccgacc caccgcatcc gctagcgaga atttccgccc
     8641 catgcgcgct tctcgctcta ccctttgttt gtagtcatcc ttgtcatcac ccatggctac
     8701 gttctccaag cccacagtct tcgtcaagaa agccccaagg taaagcgctg tgaacgaatg
     8761 ctatgggtga tgaacgggga tggtggtcac ggctggtgcg acaggttgca caccggcgtt
     8821 gacactgcag ggagggagac aaagggttat cgatcatgga ccgggaatcc tcaatcatgc
     8881 cgaatccgga aaaggggcga ggcctgcgca ccaaggtggc ttaatcatgt tggttcacgg
     8941 ctcacgctcg agcttcaggg ggtaacgaac caatcccttg gtttccacat catgactggc
     9001 cccttgaccg tcgtggctag aatcatgata tcgtattcgg ggatatacca tgggggcgta
     9061 ctttgggaag aaagaacgca agatagttgg ccaagt
//
LOCUS       DAOSOR010000188         9045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_7657_length_9045_cov_4.114016, whole genome shotgun sequence.
ACCESSION   DAOSOR010000188 DAOSOR010000000
VERSION     DAOSOR010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 9045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9045
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7657_length_9045_cov_4.114016"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PK710_11625"
     CDS             <1..362
                     /locus_tag="PK710_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10415.1"
                     /translation="IHPTRLPSIPKATTTSRFRPRRRKPKHVWKRVVPPPRFPPSESF
                     ARPMALHRSALPALPKPVEDRFLSAFAILIVGLLVALGAGLSFLIVSESRIEALAPYI
                     SQPWATSPTAPASSHIP"
     gene            582..1118
                     /locus_tag="PK710_11630"
     CDS             582..1118
                     /locus_tag="PK710_11630"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HOT10416.1"
                     /translation="MAGAYKGKALSFWLRHRTKRRRKNRTASTPRSLHDVLTTLEPEI
                     FREQLTRCPITLAQWQEAVGLRLADRSSPRRLDPDGSLLIAVKSSVWAQELSMLSSMV
                     CQRLQSQGHDVRTVRFMVSSVQPERRGPGRVETRFVPKPACLPEDLQRDLDDVQDDAL
                     REAIRAAMMASLATKQSG"
     gene            complement(1126..1959)
                     /locus_tag="PK710_11635"
     CDS             complement(1126..1959)
                     /locus_tag="PK710_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT10417.1"
                     /translation="MHFRLRYLHHDFELPPGRFLIGRSTECQLSLDDPLVSRKHALLV
                     VTPDSIELQDLGSRNGVLVNDEKISTSRDVNHGDRITIGSQSMTIYAPPEEAPTVRAF
                     DIARGDLHVTVTAMDPVRVPVSESVEPKDEGTSKRDAFRLLGGVAEKALVLGRAEEAE
                     RLLSTMLDHVMQTVREGADIDDSLIEQAGYYAAKLAGATNKGAWVNYVIELYASKRKP
                     CSAATIDELHAVFRKVKDIDLAALRAYVDALREEATKLGPADRFLVQRIEGLERLVAA
                     Q"
     gene            complement(1962..2825)
                     /locus_tag="PK710_11640"
     CDS             complement(1962..2825)
                     /locus_tag="PK710_11640"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT10418.1"
                     /translation="MARFRLRIQLQEIDLVQGDTVLGRSPECHVTIEDPLVSRMHARI
                     VIQGSQATIQDLGSRNGVRVNGEPIDTVTGLRDRDRIRIGMQEFVFLAVSPKDPRIAS
                     KSTGFLQHCERCDYPYPEGSPVCPNCGYEATREPEESTLSGVVGDNKANWASQLLLEL
                     MDRALAVGRWEEAEQVLKRARAAVDERLAIGEMDPSQLSQLLGSASKIAQTLQAEHWV
                     TWIMESCARIRRAPSADVVARLRTVPLRHHDVVIRAIDAIVEEQVPAARDEALGIAAL
                     LQWRKELSVQE"
     gene            2977..3816
                     /locus_tag="PK710_11645"
     CDS             2977..3816
                     /locus_tag="PK710_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10419.1"
                     /translation="MRPRRLELVLFLMVTVPIGLAMAQSEPSSAETSTAASASNSAIS
                     APPIERPPEQLVWLHSGAVLRGAIIELVPGKTITLQLATGEIRQIAWDEIRQHQWVEA
                     SASATPSTPPVFSSPPAVTRSGVLIHLLGDHPHLRLETQPLQGGPWRFACDAPCNQSL
                     TVYQKNLRVTGPGIRPSNPFAIDAQQGEESLQVNAGSETSHVWGQRSLTAGIVLALAG
                     GLAYGLGRLEDSDEATLGGITSMSLGGLGILVSLPLLGTSTTTVRNGKGNRVGLGETP
                     HRW"
     gene            complement(3921..5012)
                     /gene="serC"
                     /locus_tag="PK710_11650"
     CDS             complement(3921..5012)
                     /gene="serC"
                     /locus_tag="PK710_11650"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOT10420.1"
                     /translation="MARVFNFNPGPAALPLAALERAQNEFTDFKGTGMSILEHSHRGK
                     VYDAVHNEAIGLIRELLDVPEDYHVLFLQGGASQQFAVVPMNLLPAGKSADYILTGAW
                     GKKAFAEAQVVGTARLAGSTAEEKFTRVTKQQELSLDPAAAYVHFTSNETIHGVQYLD
                     AFPETGNVPLVCDMSSDFLWRKIDVRKFGLIYAGAQKNIGPSGVTCVLVRKDLVEAGR
                     TDIPKIFRYKTQADENSLANTAPTFAIYMVRNVLLWLKEFGGLAAIEKQNREKAKLLY
                     GLIDEKKGYYKCPVSPESRSVMNIVFRLPTPELEAKLIADAAAAGFVGLKGHRSVGGI
                     RVSAYNAIPLEGIQKLVAYLDDFASKNKA"
     gene            complement(5220..5969)
                     /gene="recO"
                     /locus_tag="PK710_11655"
     CDS             complement(5220..5969)
                     /gene="recO"
                     /locus_tag="PK710_11655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOT10421.1"
                     /translation="MRTFASRAATFRTQALVVRTVPFAEADLVVTLFTQALGHLAAIA
                     PRARSTSPKNRRILEPMHTLDVVLRDRPAGALLSLVSSQIARPRLGILSDLDRMKTAG
                     TVLRWVRQTAPYRTPETDLWKLIEELLDALDQTALRASPRALLVVAGFRLLECLGYGL
                     DLGACVVCGRPCPPERPAFVDPVRGGLVCRRCGGAGRLLSGAIRQRMLLALQGQAEVL
                     EQDIAPLLGLLEAALVAHTNIRPEGAEAISR"
     gene            complement(5974..6402)
                     /locus_tag="PK710_11660"
     CDS             complement(5974..6402)
                     /locus_tag="PK710_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT10422.1"
                     /translation="MESIGKYLKRRREARGMSVGELSRATRIPAAALCRIESDQFDDL
                     PGEVFVRGFLKAYARAVGLETDEVLARYTSSRRVAWVTPLPFNTPIERPPTRRFGVAI
                     AFVLLLILFTLAVSIVLKPRGRDLPQELSQAEPSAFTLTG"
     gene            complement(6451..7311)
                     /locus_tag="PK710_11665"
     CDS             complement(6451..7311)
                     /locus_tag="PK710_11665"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT10423.1"
                     /translation="MIRTRQQKAGQPTLRGMVGRLCVGLGWVLPVILGLACGPSRDAQ
                     SPGAADPERQSIADYDVARDLFVARGNAREALKVALRAVELDDENADAAHLVSLIYLY
                     FCASSEADCRLDEAERYVRMAIKAKADLREAKNTLGVVLIEKKRYAEAVEVLEPLTKD
                     ILYSTPETAWGNLGWAYFLKGDTDKAIESLRRALALQPEFCVGAYRLALAYEKKGDHK
                     AALESVTRALETDRPECRGLQDAFRARARIAMRLGDHESARGDLERCRDLDAETSSGR
                     ECAAALAKWR"
     gene            complement(7739..8452)
                     /locus_tag="PK710_11670"
     CDS             complement(7739..8452)
                     /locus_tag="PK710_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOT10424.1"
                     /translation="MQDFGAAEKERLLARFGRPFEKGDVIFREGEQATEAYLLQEGSV
                     RLVKRVRAMERSLMVLKPNDLFGETALVEGAVRTSTTIALSDGVSLALDPSTFRRLLE
                     TSPAISLRLVQQLVRRLSDAEDQIEIMMLRDSQAKVVSVLLKLARAEQEPGTNTRILE
                     VSPMDLSSRAGLDVDTVKRGVHQLREGRYVKIVDERIEIPDMEALTKLYALLTLKDEI
                     RGSHSSHPPDDRGEGEDVR"
     gene            complement(8511..>9045)
                     /gene="tsaB"
                     /locus_tag="PK710_11675"
     CDS             complement(8511..>9045)
                     /gene="tsaB"
                     /locus_tag="PK710_11675"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOT10425.1"
                     /translation="EVLERSGAGLESIDRLAIGMGPGSFTGVRVGMGFAKGLGFARGI
                     GGVGVMSLEAMAYAARRIVGARRVFSLLDAKKGEVFVASFDGQGRRGLGPEHLARDRV
                     QRWMDTVEPWGKRVVVGEVAEELDLRNVELIRDRSCDLPGAEAMAALAVARAASDDLA
                     LLEPLYVRPPDITQPAG"
BASE COUNT         2029 a         2918 c         2258 g         1840 t
ORIGIN      
        1 gcattcaccc cacgcgtttg ccgtcgatac cgaaagccac aaccacatcc cgttttcgac
       61 cgcgtcgccg caaacccaaa cacgtgtgga aacgtgtcgt tcctccacct cgattccccc
      121 cttccgagtc gttcgccaga ccgatggctc tgcaccgatc agccctccct gccttaccca
      181 aaccggtgga agaccgcttt ctcagcgctt tcgccatatt gatcgtcgga ttgctcgtcg
      241 ccttgggagc aggcctgagt ttcctcatcg tctccgaatc acgcatcgaa gctctcgcgc
      301 cctatatctc gcagccctgg gctacctccc ccaccgcacc cgcatcatcc cacatcccct
      361 gaggcgtatc ccttcgcccg caaaaacaag gaataccacg gcaagttgat gagccgacag
      421 aaccacccgc gtaacaaaga ttatgcttgc catctggtga actcatccca gcgctctgcg
      481 ttcagaatgc gaaacccatc gacgcgacag ccgtgaagca ttaggttttt tcagcggaaa
      541 gacatagctg tccatcaagg accatcgcag ggatgaagag catggccggt gcctataaag
      601 gaaaagcgtt atccttctgg ctcaggcata gaacgaaacg tcgccgtaag aaccgtactg
      661 caagcactcc acgttcactt cacgacgtgc tcacgaccct cgagcccgag atctttcgcg
      721 agcaattgac acgatgtccc atcaccttgg cacaatggca agaagccgta ggacttcggc
      781 tcgccgatcg ctcatcgccg cggcggctcg accctgatgg ctccctattg attgccgtca
      841 aaagctcggt gtgggcacaa gagctgtcca tgctctcgag catggtttgc cagcggctac
      901 aatcacaagg tcacgacgtg agaaccgtcc gtttcatggt gtcgtctgtg cagcccgagc
      961 gacgtggacc tggtcgagtc gagacgcgat ttgttcccaa gccagcgtgt cttcctgagg
     1021 atttgcagcg cgatctcgat gacgtgcagg atgatgcgct tcgagaggct atacgtgcgg
     1081 ccatgatggc tagcttggcc acgaaacaaa gcggttgaat tcgattcatt gtgcggcaac
     1141 cagccgctcg agcccttcga tgcgctgaac gagaaaacga tcggccggtc caagcttggt
     1201 cgcctcctcg cgtaacgcat ccacataggc tcgcaacgca gcaaggtcga tatctttgac
     1261 tttgcgaaac accgcatgca gttcatcgat cgtggccgca ctgcatggtt tgcgcttcga
     1321 agcataaagc tcgatcacgt aattgaccca cgctcctttg ttcgtagctc ccgccaattt
     1381 tgccgcataa taaccagctt gctcgatgag gctatcgtcg atgtctgctc cttctcgaac
     1441 cgtttgcatg acgtgatcga gcatggtgga aagcaaacgc tcggcctctt ccgcgcgacc
     1501 aagaacaaga gctttttccg cgactcctcc cagcagtcga aaggcatccc gtttgctggt
     1561 tccttcgtct ttcggctcga ccgactccga cacgggaacg cgtaccggat ccatcgctgt
     1621 caccgttaca tgcaaatccc ctcgagcgat atcaaaggcc ctcaccgtcg gcgcttcctc
     1681 tggaggagcg tatatcgtca ttgactggga gccgatggtg atccgatcac catgattgac
     1741 atcgcgagac gtcgatattt tttcatcgtt gaccagcaca ccgttacggc tccccaagtc
     1801 ctgaagctcg atggaatcgg gcgttacgac aagaagagcg tgttttcgac tcacgagcgg
     1861 atcgtcgagc gatagctgac actccgtact ccttccgatg agaaaccgcc cgggaggcaa
     1921 ctcgaagtcg tggtgcagat atcgaagacg gaaatgcatg ttcactcctg tacggagagt
     1981 tctttacgcc actgcaggag ggcagcaata ccaagagcct catcgcgcgc ggccggaact
     2041 tgttcctcga caatggcgtc aatggcccga atcacgacgt cgtgatggcg aagaggaacc
     2101 gtgcgaagac gcgcaacgac atcggcggat ggagcgcgac gtattctggc gcagctttcc
     2161 atgatccaag ttacccaatg ttcggcctgc agggtctgcg cgattttcga agcgctgccc
     2221 aaaagctgac tgagctggga ggggtccatt tctcctatgg caagacgctc atcgacagcg
     2281 gcacgcgccc ttttcaatac ctgctcagct tcttcccaac ggcctacggc cagcgctcga
     2341 tccatgagtt cgagtaacaa ctgggaagcc caattggctt tgttgtcccc gacaacgccg
     2401 gacaaagtgc tttcctccgg ctctcgagtc gcctcatacc cgcaattcgg acatacaggg
     2461 ctaccctccg gatacggata gtcgcatcgt tcgcaatgct gaagaaaacc cgtggatttg
     2521 ctggcaatgc gtgggtcttt cggcgagacg gccaaaaaca cgaactcctg catcccgatg
     2581 cggattcgat cccgatctcg caacccagtc accgtatcaa tcggctcgcc attgacccgt
     2641 acaccattgc gcgaaccaag atcttggatc gtcgcttggc tcccttggat gacgatgcgc
     2701 gcgtgcatac gggatacgag cggatcctcg atggtgacat ggcattcggg acttctcccc
     2761 agcacggtat caccctgcac aagatctatc tcctgcaact ggattcgaag tcgaaaacgt
     2821 gccaaggccc agcctcaatc tatgacggac agcctcatca gcgtagcaac gcaagggcac
     2881 gtacgtcaaa gggtgcaagc cctatccgaa catccatgag accatgaggc tgcggaactg
     2941 gttgcagcca aggctagaac gtggtcaagc ctacgcatgc gcccacgaag attggaactc
     3001 gtgctttttc ttatggttac ggtacccatc ggccttgcga tggcgcaatc ggaaccaagc
     3061 agcgctgaga cgtccacagc cgcttccgcc tccaactccg ctatttctgc gccccccatc
     3121 gagcgtccgc cagagcaact ggtctggctt cactcgggag ccgtactgcg gggcgccatc
     3181 atcgaactcg tccctggcaa gaccatcacc ttgcagttgg ccacaggcga aatccgccaa
     3241 atcgcttggg atgagattcg acaacaccag tgggtggaag catctgcttc cgcaacaccg
     3301 agcacaccac ccgttttttc ttcaccaccg gccgtcaccc gctcgggggt gctcatacat
     3361 ttgcttggag accatcctca ccttcgcctc gaaacacaac ctttgcaggg cggcccttgg
     3421 aggttcgctt gcgatgctcc atgcaatcaa tccctgaccg tctaccaaaa aaaccttcgc
     3481 gttaccggcc ccggcattcg accttccaat ccatttgcga tcgatgcgca gcaaggcgag
     3541 gagagcttac aagtcaacgc aggctctgaa acgtcgcatg tatggggcca acgctcgttg
     3601 accgcgggaa tcgtgcttgc tctggcaggc ggcctggcct acggactcgg ccgtctcgaa
     3661 gactccgatg aagccaccct aggaggcatc acctccatgt ctctcggcgg tcttggtatc
     3721 ctggtctcgc ttccgttgct cgggacgagc acgacgactg ttcgaaatgg gaagggaaat
     3781 cgggtgggct tgggcgaaac gcctcatcgg tggtagccat gagacgggca tgggcatcgc
     3841 ttggccttcc catcagcagg aaatagccgc catgcgaagc cccacgccac catgagcaaa
     3901 gctctctcgg agcccttgcg ttatgctttg ttcttcgacg caaaatcgtc gagataggcc
     3961 accaacttct gaatgccctc gagcggaatc gcgttgtacg cggaaactcg aattcctccc
     4021 acgcttctat gtcccttcag gccgacaaac ccggctgccg cggcgtccgc aatgagtttg
     4081 gcctcgagtt cgggcgtcgg aagacgaaac acgatgttca tcaccgaacg gctctccggg
     4141 gaaacagggc atttatagta gccttttttc tcgtcaatca gcccatagag cagctttgct
     4201 ttctcgcgat tctgcttctc gatcgccgcg aggccgccaa actctttgag ccaaagcaag
     4261 acgttgcgaa ccatgtaaat cgcaaacgtc ggcgcggtat tcgcgaggga gttctcatcc
     4321 gcctgtgtct tgtagcggaa aattttcgga atatccgttc gtccagcttc gaccaggtcc
     4381 ttgcgcacga gcacacaggt cacccccgaa ggaccaatgt ttttctgcgc tcccgcataa
     4441 atgaggccga attttcggac atcgatcttt cgccagagaa agtcactgga catgtcgcag
     4501 acgagcggca catttccagt ctccgggaaa gcatcgagat actgcacgcc gtgaatcgtc
     4561 tcgttcgagg tgaaatgcac ataggcagca gcggggtcga gggaaagctc ttgctgcttg
     4621 gtcacgcgcg tgaacttctc ttcggccgtg gagccagcaa gccgcgcggt cccaaccacc
     4681 tgcgcctccg caaacgcttt ctttccccac gctccggtca ggatgtaatc agcgctcttg
     4741 cccgcgggca atagattcat cgggaccacg gcgaattgct ggctcgctcc cccctgcaaa
     4801 aaaagcacgt gatagtcttc aggaacgtcc aatagctccc gaatcagacc aatcgcttcg
     4861 ttgtgaaccg cgtcgtagac ctttcctcga tggctatgct cgagaatgga cataccggtt
     4921 cctttgaagt cggtgaattc gttctgggca cgctcgagcg cagcgagcgg tagggccgcc
     4981 ggaccagggt tgaagttgaa tactcgggcc atggctttgt cctttcccta agcacaatgc
     5041 ccaatggggg cttcgtgctc tgtcatgttc gttccacatc cgaacggaac ggccccctta
     5101 tacagcctca gttcccattt tgcacgcctc gtcgacctcg tcaggaaccc gggaaaattc
     5161 cacagcgagg cacgttctgg ccgcggtccg acaccgaaac gacaagcccc gagctgtcct
     5221 caacgcgaga tggcttctgc cccttccggc cggatattgg tgtgggctac caaagcagcc
     5281 tccaaaagcc cgagcaacgg cgcaatatcc tgctccaaca cctcggcttg cccttgcaaa
     5341 gcaaggagca tccgctggcg gatcgctccc gacaacaaac gccccgcacc accgcatcga
     5401 cggcatacca agccaccgcg aaccggatct acgaaagccg gacgctcagg ggggcatgga
     5461 cgcccacaca ccacgcacgc gcccagatcc aaaccatacc ccaaacactc gagcaatcgg
     5521 aagccagcca cgacgagcaa ggcccggggg gaggctcgaa gagccgtttg atccaatgca
     5581 tcgagcagtt cttcgatcaa tttccaaagg tcggtttctg gcgttcgata gggagccgtt
     5641 tggcgaaccc accgcagcac ggtgccagcc gttttcattc gatcgagatc ggacaggatc
     5701 cccaggcgcg gacgagcgat ctgggaggat acgagcgaca agagagcgcc cgcgggtctg
     5761 tcacgcaaca cgacgtcgag cgtatgcatg ggttcgagga ttcgtctgtt ttttggggag
     5821 gtcgatcggg ctcgcggggc tatggcagcc agatgaccga gggcttgggt aaaaagggta
     5881 acgacgagat cagcctccgc gaaggggacc gttcgcacga ccaacgcctg cgttcggaaa
     5941 gtcgcggccc gcgaggcgaa ggttcggatg atttcagcct gtcagggtga aagccgaggg
     6001 ttcggcctgg gatagttctt ggggaaggtc ccttccacga ggcttgagca cgatgctcac
     6061 tgccagcgtg aacagaatga gtagaagaac aaaagcgatc gcgactccga aacgacgggt
     6121 gggcggccgc tcgatcgggg tgttgaaagg tagcggagtc acccaagcca cacgacgact
     6181 cgacgtgtaa cgcgccaaca cctcgtcggt ttccagcccg acagcacgag cataggcttt
     6241 gagaaagccg cgaacgaaga cctctccagg caggtcatcg aattgatcgc tctcgattcg
     6301 acacaacgct gcggccggga tgcgggtggc gcgagatagt tctcccacgc tcataccgcg
     6361 cgcctcccgg cgccgcttga ggtatttgcc gatcgattcc atggtacctc tccacatcac
     6421 ccctctcgta aaagaagggc gatgctttgc tcatctccac tttgccaatg cggcagcaca
     6481 ctcgcgaccg gatgacgttt ccgcatccaa gtcccgacac cgctcgagat cccccctggc
     6541 cgattcatgg tcccccagac gcatggcaat tcgagccctg gcccgaaagg catcctgaag
     6601 acctcgacac tcgggacgat ccgtctccaa agctcgcgtc acagattcca acgcggcttt
     6661 gtgatcacct tttttttcat aagccaacgc aagacgataa gcaccgacac agaactcagg
     6721 ctgcaaagcc aacgcgcgac gaaggctctc gatggccttg tcggtgtcgc ctttcaagaa
     6781 ataagcccag ccaagattcc cccaagcggt ttccggcgtg gaataaagaa tgtccttcgt
     6841 cagtggctcg agtacttcca ccgcctccgc ataccgcttt ttctcaataa gaacgacacc
     6901 aagggtattt ttggcctcac gcaagtccgc tttggccttg atcgccatgc gaacataacg
     6961 ctccgcctcg tccaaccgac aatccgcctc cgatgatgca caaaaataca aatatataag
     7021 cgatacaaga tgcgccgcgt cggcattctc gtcgtccagc tctaccgccc gaagcgccac
     7081 cttcaacgcc tcgcgcgcat ttcccctcgc tacgaaaaga tcccgcgcga catcatagtc
     7141 cgcaatcgat tgacgctccg gatcagccgc tccaggagac tgcgcatcac gcgacggccc
     7201 acaagccaag cccaaaatca caggcaatac ccaccccaac ccaacacaaa gccgtcccac
     7261 catcccccgc aaggtcggtt gtcccgcttt ttgttgccga gttcgaatca tctttcactc
     7321 gtggttggaa tcgctcatcc caaaaacaac gacagtctat tcaaatcctc ccaaaaccat
     7381 ccctatggaa tccaagtacc gctcgctcct ctgccaaatc aaggtgtgcc tgtggttcac
     7441 actcatcatg ccgcaagtac cgcagcctcg acatgccgtc aactggatca cttcgatccc
     7501 ctgcggtttt gttcacaata ttcttattca taatgaaacg aatattcttg aataaacatc
     7561 taagtatagg aaattattga agattaatat catacgcgag ccatgaatcc caccactcgc
     7621 ccctggacga tttggccaac ttttttgagg accatcccct gctccccaga cgctccgcgt
     7681 cattcatgaa aacgacgcac cgcgtccatc cgcaacgatt ccccccgcac cctatttctc
     7741 agcggacgtc ttccccctca ccccgatcat ccggtggatg ggaactatgc gaaccccgaa
     7801 tctcatcttt tagcgtcaac aacgcataaa gcttcgtcaa cgcctccatg tcgggtattt
     7861 cgatgcgctc gtctacgatc ttgacatatc gcccctcacg caattgatga acgccacgct
     7921 tcaccgtatc cacgtcgagc cctgcacgac tgctcaggtc catcggagat acctcgagga
     7981 tcctcgtgtt cgtccccggc tcttgctcgg ctctggcgag tttgagcagc acgctcacca
     8041 ccttggcctg ggaatcgcgc aacatcatga tttcaatctg gtcttccgca tcactaagcc
     8101 tgcgaaccag ttgttggacc aaccgcaggg agatggcggg gctcgtttcg agaagccgac
     8161 gaaatgtcga tggatccagg gcaagactta caccatccga caaggcgatg gtcgtggaag
     8221 tccgaaccgc tccctccaca agcgccgtct cgccaaataa atcgttgggc ttgagcacca
     8281 tcaggctccg ctccatcgcc cgcacacgtt tcaccaaccg tacggagcct tcctgaagaa
     8341 gataagcctc cgtcgcttgc tctccttcgc ggaaaatcac gtcgcctttt tcgaaagggc
     8401 gcccaaaacg agcaagaagg cgttcttttt cagcggctcc aaagtcctgc atatcatcca
     8461 tcctaaaccg aacgactccg tatcgtcgaa tttcgaattc ggatagcccc ctaccccgcc
     8521 ggttgggtga tgtccggcgg gcgaacgtat aacggttcca gcaaagcgag atcatcgctc
     8581 gccgcgcgcg caaccgcaag cgccgccatc gcttccgccc ccggcaaatc acaagaccga
     8641 tcgcgaatta gctccacatt ccgcaaatcc agctcctccg ccacctctcc cacaaccacc
     8701 ctcttgcccc aaggctccac cgtatccatc caccgctgca cacggtcacg cgccaaatgc
     8761 tccggcccaa gaccccgtcg cccttgccca tcaaaactcg ccacgaatac ctcccctttc
     8821 ttggcatcaa gaagcgaaaa cacccgtcga gcccccacta tccgtcgcgc tgcataagcc
     8881 atggcttcca acgacatcac ccccaccccg ccaatccctc gcgcaaatcc caatcccttc
     8941 gcaaatccca tccccacgcg aacccccgta aacgatcccg gacccatgcc gatcgcaagc
     9001 cggtcaatcg actccaaccc cgcaccagaa cgctccaata cctcc
//
LOCUS       DAOSOR010000189         8950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_7768_length_8950_cov_4.032940, whole genome shotgun sequence.
ACCESSION   DAOSOR010000189 DAOSOR010000000
VERSION     DAOSOR010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8950
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7768_length_8950_cov_4.032940"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(776..1117)
                     /locus_tag="PK710_11680"
     CDS             complement(776..1117)
                     /locus_tag="PK710_11680"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOT10426.1"
                     /translation="MSNQSAEITPSVAHEILSLIEVAPGAVVSRTISKTKGGTLTLFA
                     FDAGEGLSEHAAPFDAFVQVLDGKLTLKIGGVPVEATPGTLVRMPANIPHGLHADAPT
                     RMMLFMLRDPN"
     gene            complement(1190..1771)
                     /locus_tag="PK710_11685"
     CDS             complement(1190..1771)
                     /locus_tag="PK710_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOT10427.1"
                     /translation="MAVTPSTMLPLGTRAPGFSLLNAVDGTRVALSDFREKRALLVMF
                     VCNHCPYVVHVREELTRLGADYLPQGVGIVAINANSSKTHPQDGPSAMKQLAQQLGWQ
                     FPFLFDETQQVAKAYRAACTPDFFLFDDRTRLVYRGQLDDSRPGNGIAVTGKDLRAAI
                     DALLAGRAVSDQQKPSIGCNIKWNAGNEPDYFG"
     gene            complement(1843..3051)
                     /locus_tag="PK710_11690"
     CDS             complement(1843..3051)
                     /locus_tag="PK710_11690"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOT10428.1"
                     /translation="MTRRTIAIAGWLVTLLVSGTASANGFFLWEASPSSVGQGGATVA
                     DGSEPATLLFNSAGLTRLDGFQLQANLYTYIANNTWKDPNTGEKQDAETGVFPIPSLF
                     ASYKPIDWLSVGVGGYSTFGLSLGWPDRWKGSTISQKSSLRSYSVQPAVAVGPFSGFS
                     LGAGLTVTRGSVKLERGLRLGNDYGTSTLGGVDTGYAPSVSVLFEAKEWFRFGAQYRH
                     KTVMTLDPGKIDFDVPAAYGQQLRDQNLTASITLPAMTQFGLRMQPGKNIEFEFDGFL
                     ILWHTYDELAFKFEDPTINQTQKTNWHDAWEWRIGGQYTMDELALRAGFIWDSTPIPD
                     ETLDPLLPDNHRMIPSVGVGYDFGMVRADAAYQFVYLLPREVNEPVNKFPGKYNAIVH
                     TIALGATMRL"
     gene            complement(3079..4494)
                     /locus_tag="PK710_11695"
     CDS             complement(3079..4494)
                     /locus_tag="PK710_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10429.1"
                     /translation="MLGSRRFALLWGVTLVVGGCDPNLGRDEPMEATRPIFDPAAGYV
                     PLPNDALLDPASGLLNIPVDESVDSALTQHIKGNLNRIDGWIPGSMITIPFDDKLDPS
                     SLTGDSVRLYDVTQVQSSASSLVRVDPSSYYVAFNVGREPAVQPPYTLYVRMKPVPLL
                     PPDFEMGHRYMVVVTNDVKDMQGRRVVGTPAMELLKSQTPLVNSFGRSQTILPDEDAA
                     ALEVLRLANAPALAAIGEEIDLATVVAHAGFTIQSNPMPAFNPMLLGYELPKPIGDPS
                     AANSKFGKPWVCFHHPIDGKTVSGHVKLFKRTPALVEVSMQASVGKVMNPDSAEKVLC
                     EQAVIIDAGALEESTTYQVVLTDGIKGLNGAASRQSSIFSLMASPTPLLDSSKTPVGL
                     NSPYIDSTFDALLTTGKDPSSATQEDWDKAYDMLVSDSALGTVEKWRQTYQPWLQDAA
                     SALGVERASITVTWVFTTAAP"
     gene            4651..4896
                     /locus_tag="PK710_11700"
     CDS             4651..4896
                     /locus_tag="PK710_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10430.1"
                     /translation="MGFNQEIPSTPRRFTLLPPPPTHPIAYEICQDSGWDWDEDSVFE
                     ATSTSSSPTLWQAPPTECGALAVDDIPYRTIASALAL"
     gene            complement(4909..6456)
                     /locus_tag="PK710_11705"
     CDS             complement(4909..6456)
                     /locus_tag="PK710_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOT10431.1"
                     /translation="MYCRCFAFIFSMTRTMGWIAAACIAVGCGNDSENPGPAAGGAGA
                     SDGGQVQSDAGGQGGTQGLEEAGTGGASGTGATGTAGASGGYTPEGGTEDVVADVMID
                     VPEDAVGSLDCERLVALDAALVAASNEGEKVSLLEQTLVDVEKSDGFPIRCAPDATFF
                     FRRPQGWGHPHLVGEFNGWDPTATPMVLVAEDLYRVTVAVDTGPHRMLYKFSDGVHWV
                     ADPMARRFGFDEHGEYSLVQGGNQRGHLERYLGVAGNGLSARPLTVYVPPGYEQSTQS
                     YPVLYAHDGQNLFDPGSIWGGWHLDDVIENLLSSGEIKPLIVVGVHNTTARMDEYTHV
                     QDVVSGQTVGGAAEMYFGLLATRVMPLVASHYRVKAGPENTWTMGSSLGGLVSLYVTL
                     KHPDVFGKAAGLSSTLGWGSIQAHNPTLIEMVPSLGKPDVVLYLDSGGTNGGGCVDSD
                     GDGIRDDNDMASDNYCETEQMRDVLAAEGYEFNKDLFHWHEMGAPHNEAAWSARVFRP
                     LRVLAAP"
     gene            complement(6679..8724)
                     /locus_tag="PK710_11710"
     CDS             complement(6679..8724)
                     /locus_tag="PK710_11710"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HOT10432.1"
                     /translation="MTESQKAKGLTREMQRDRFGCVEQRGSDGKGALKRDRLEGRTKR
                     MLTHRLQDKPWMKYVSTGILLASASLWAACSSDEGSVDKRSEVTNEDGDAPLSNLDDL
                     TAGAPSKADLPEEGKADAVYPPTYFDLVKLQSPVKSQGSRGVCSIFASTAHMEHLYIA
                     EGTIKTPDFSEQFLQWSVKNEVKAFRNTEGSSASENLRAIYQFGIVEEAAWPYESTKW
                     SSYNHPECSGEDSLPTECYTNGNPPEAAMGAKRWRIPQGRWINSNERSIKAHMFSKKT
                     AVEVGGTFYYQAWNHRGSPLPTNNAYWRKGYVTYPNDVDKQKSLEKRAGHGFLLVGWD
                     DTLEVQKRDGDGKLLTDSQGNPLMEKGFFIFKNSWGKGSFGVDNPHGDGYGYISYKYV
                     AELNAYVSGLPKIELPKEVCNDGEDNDMDGKTDCDDPDCAQDPSCVGSTQVFSGTGGA
                     IPDNDPKGLTSSIVVAKGGTISAMSVAVDITHTYSGDITIKLVREGGGEVVLQDRDGG
                     SEHNIKRVFGISDFNGQDAVGTWKLVVIDNAKLDTGTLNGWTLSITDCVGNDCASGAK
                     TYSNTESKPIDDGSSTGVFSNIVVDDEGEISAMKVFVDITHPEQIDLTIKLQRVGVPG
                     EAILQEAASADGPYVPRSYNVSNFLGQDAKGTWRLVVVDEAAGDSGTLNGWSLEIAR"
BASE COUNT         2054 a         2900 c         2104 g         1892 t
ORIGIN      
        1 ggctcaaaga ccggtttgct tgagcgtcag tttgaccggg tgatcaatca cgcccggtga
       61 gtgggaaaat cgaatacccc tcgccagtca agtcgaacct cggctccgtc ggccaacagc
      121 gtcgtcgcca cgcacggtga ccccagcatg cagactgtag ccgtcgatgt cggcgcaaaa
      181 cggctgggga gtgagcagta cgagtcgaga tctcgctttt ttcgaaagct tcgttcacta
      241 ttgtgtagaa gggaagacct gctcgcgtct cggaccggtg acctgcctga gccatcccct
      301 catttttccc ggactatttc ccttgttgtc gactgccgga aagtcgtacc catcaccact
      361 cggccaaggg gttcaagacc aggtcaaact caaggttttg gggtccacgt ccacgttcaa
      421 gttcatgtca aggaccaggt caatgtcaat gtcaatgtca atgtcaaggc caaggtttag
      481 gggattgtct cccatatccc ttgtttttca ggaaataatg aggggatctt tcagattacc
      541 cctgccttac ctcgatgagc cgacagcgag catccacccg aaatgggata acgatagtac
      601 accaccgaac caggtatgcg cccaacacga cacgaagtgc ctgctggcac catgcggggg
      661 tcatccggtc cgaggcgccg atcgtcatcc gcaacggtgg gccgactcgg gtattggtgt
      721 gatgaggatg gtcatcgcag tccgtgggcg caaatcaggc ttcgccccga ctgcttcaat
      781 tcgggtcgcg caacatgaac aacatcatgc gggtgggcgc atccgcatgc aaaccatgag
      841 ggatgttcgc aggcatacgc accaaggtcc ccggcgttgc ttctacaggc accccaccga
      901 ttttcaacgt aagtttgcca tccaacacct gcacaaacgc atcgaaaggc gccgcgtgct
      961 cacttagccc ctcgcccgcg tcgaaagcaa atagcgtgag cgtgccccct ttggtcttcg
     1021 aaatcgttcg gctcacgacc gctcctggcg caacctcgat gagagatagg atttcatgag
     1081 ccaccgacgg ggtaatctca gcagattgat tggacatgag ttctccttgt aaaggtcaac
     1141 ggaaccaata accgcaaagc tcctcctgcg ccatgacgct tgtcatgggt cagccaaagt
     1201 agtccggttc gtttcccgca ttccacttga tgttgcagcc aatgctaggc ttctgctggt
     1261 cactgaccgc acggcccgcg agcagcgcat cgatcgccgc ccgtagatcc tttcccgtca
     1321 ccgcaatgcc gttgcccgga cgagaatcat cgagttgccc tcgatacacg agccgagtcc
     1381 ggtcatcgaa aagaaaaaaa tccggggtac aagccgctcg ataggccttg gctacctgct
     1441 gtgtctcgtc gaaaagaaac ggaaactgcc agccgagttg ttgcgcaagc tgcttcatcg
     1501 ccgaaggtcc atcttgagga tgtgttttgc tgctgtttgc attgatcgca acgatcccga
     1561 ctccctgggg gaggtaatcg gcaccgagcc tggtcagctc ttcgcgcaca tgaaccacgt
     1621 agggacagtg gttgcacacg aacatcacga gcagcgcgcg tttttccctg aaatcactca
     1681 gcgccacgcg cgttccatct actgcattca gcaaagaaaa gccgggcgca cgcgtgccca
     1741 acggcaacat ggtagaaggt gtaacggcca tcgcgatgtc cttgcgctgc ttcgccccgg
     1801 ggtcaagccc ttccgcagat cacttgccct gcccctgtct gtctaaagtc tcatcgttgc
     1861 acccaaagcg atcgtatgaa cgatcgcatt gtactttccc gggaacttgt tgactggctc
     1921 attcacttcc cgaggcagca agtacacgaa ttgataagcc gcgtccgcgc gaaccattcc
     1981 aaaatcataa cccacgccca ccgagggaat catgcggtga ttgtcgggaa gaagcggatc
     2041 caatgtctca tctggtatgg gagtcgaatc ccagatgaat cccgcgcgaa gcgcaagctc
     2101 atccatcgtg tattgccccc cgattcgcca ttcccacgca tcgtgccaat tcgttttctg
     2161 tgtctgattg atcgtgggat cttcgaactt gaacgccaac tcatcgtagg tgtgccacag
     2221 aatcaaaaat ccatcgaact cgaattcgat gttcttgccg ggttgcatcc gcagaccaaa
     2281 ctgcgtcatg gcgggaagcg tgatcgatgc cgtaagattc tgatcgcgaa gctgctgacc
     2341 gtaggcggcg ggaacatcga agtcgatctt ccccggatcc aacgtcatga cggttttgtg
     2401 ccggtattgc gctccgaagc gaaaccattc tttggcttcg aaaagtacgg atacacttgg
     2461 cgcatatccc gtatcgactc cgccgagggt tgatgtgcca tagtcattgc ccagcctaag
     2521 accccgctcg agcttcacgg aaccgcgggt taccgtgagc cctgcgccca acgagaatcc
     2581 actgaagggt ccgacggcca ccgcgggctg cacggaatac gatcgcaacg aggatttttg
     2641 cgaaatcgtc gaccccttcc aacgatccgg ccacccaagc gaaagaccaa acgtggagta
     2701 gcctcctaca cccacagaaa gccaatcgat gggtttgtac gaagcgaaca acgagggaat
     2761 gggaaaaacg cctgtttccg catcttgttt ttcacccgta ttcggatcct tccaagtatt
     2821 gttcgcgatg taggtgtaga ggttcgcctg aagttgaaaa ccgtccaacc gcgtcaaccc
     2881 cgcactgttg aacaacagcg tggccggttc ggaaccgtcc gccacagtgg ctcccccttg
     2941 tcccacggaa ctgggactcg cttcccaaag gaaaaaaccg ttcgccgaag cagtcccgga
     3001 aaccaagaga gtcaccagcc atccagcgat ggcaatcgtt cgtcgggtca tcatcgtcct
     3061 ctatcccgtc cgatcccctc agggagccgc agtagtgaaa acccaagtga ccgtaatgga
     3121 tgcgcgctcg acacccagcg ccgaagccgc atcctgcaac catggttgat acgtctgtcg
     3181 ccacttctcc acggttccga gcgcgctatc gctcacgagc atgtcatacg ctttatccca
     3241 atcctcctgc gtcgcggaag aagggtcttt gccggtcgtg agcagcgcat cgaatgtcga
     3301 atcaatatag ggtgaattca acccaacggg cgtcttgctc gaatcaagta gtggtgttgg
     3361 tgaagccatc aacgaaaaaa tactcgactg cctagaagcc gcaccgttca atcccttgat
     3421 cccatccgtc aacacgactt gataggtcgt cgactcctcc aacgcgcccg catcgatgat
     3481 caccgcctgc tcgcatagta ctttctcagc actatcagga ttcatgacct tgcccacgga
     3541 ggcttgcatg gatacctcca ccaacgcggg cgttcgcttg aataacttca catgacccga
     3601 aaccgtcttg ccatcgatgg gatgatgaaa acaaacccac ggtttgccga acttgctatt
     3661 cgccgcgctc ggatcaccaa tgggtttggg aagctcatag cccaaaagca tcggattgaa
     3721 tgcgggcatc ggattggact gaatggtaaa tccggcatgc gccaccacag tcgcaaggtc
     3781 aatctcctcg ccaatcgcgg ccaacgcggg cgcattcgcc aaccgcaata cctcgagcgc
     3841 cgccgcatcc tcatccggaa gtatcgtttg cgatcgccca aacgaattga ccaacggcgt
     3901 ttgcgacttc aatagctcca tggccggtgt acccaccacc cgacgtccct gcatgtcctt
     3961 tacgtcgttg gtcaccacca ccatgtaccg atgtcccatc tcgaagtccg gtggaagcaa
     4021 cggtactggt ttcatcctca cgtaaagcgt gtagggcggt tgaaccgcgg gttctcgacc
     4081 cacgttgaac gccacatagt aggaagacgg atccacacgc accagcgacg aggcagaaga
     4141 ctgcacctgc gtgacatcgt ataaacgcac ggaatcccca gtcaaactcg acggatcgag
     4201 cttgtcatcg aacggaatcg tgatcatcga gccgggaatc caaccatcga tccgattcaa
     4261 gttgcccttg atatgctggg tcaatgccga atccacggat tcatcgaccg gaatgttcaa
     4321 caggcccgac gcagggtcga gcaacgcatc gttgggtaag gggacatacc ccgccgcagg
     4381 atcgaaaatc ggacgcgtag cctccattgg ctcatctcga ccaaggttgg gatcgcatcc
     4441 cccaaccaca agggtcactc cccaaagcag agcaaaacga cgactgccta acatgcccca
     4501 ggctcctccc ggtaccaatc atcgaacgtg aagtagcgcg agcataagac ccacgcgaac
     4561 cgggcgccag caaaagaacc acacgattta tttgcacgtc atgattcaaa aacttggcca
     4621 ctttttttcc gccatgccag acggacgatc atgggcttca atcaggaaat cccaagcacc
     4681 ccgcgaagat tcaccttgct tccccccccg cccacgcacc ccatcgctta tgaaatatgc
     4741 caggattcag gctgggattg ggatgaagac agcgtcttcg aagctacctc gacttcgagc
     4801 agtcccacgt tatggcaagc cccccccacc gaatgcggcg ctctcgctgt cgatgatatc
     4861 ccgtatcgca cgatcgcttc ggccctcgcg ctatgagacc tcacggcatc aaggcgccgc
     4921 caaaaccctc aacggcctaa acaccctggc actccacgca gcctcgttat gcggcgctcc
     4981 catctcatgc caatgaaata aatctttgtt gaactcatac ccttccgcgg ccaacacatc
     5041 gcgcatctgc tccgtctcgc aatagttgtc cgaagccatg tcgttatcat cgcggatccc
     5101 atccccgtca ctgtccacac atccaccccc attggttcct cccgaatcaa ggtacaatac
     5161 gacatccggc ttccccaacg aaggaaccat ctcaatcaac gtgggattat gcgcctggat
     5221 gctcccccaa cccaacgtcg aagacaaccc cgctgccttt ccaaaaacat ccggatgctt
     5281 caacgtaacg taaagcgaaa ccaatccccc aagcgacgat cccatcgtcc aagtattctc
     5341 cggtcccgct ttcacacgat aatgcgatgc caccaacggc atcacccgcg tcgccaacaa
     5401 cccaaagtac atctccgctg ccccccccac ggtctgcccg gaaaccacat cctgcacgtg
     5461 cgtgtactcg tccatacgcg ccgtggtgtt atgcaccccc accacgatca aaggcttgat
     5521 ttcgcctgat gacaacagat tctcgatcac gtcatccaag tgccagcctc cccatatcga
     5581 cccaggatca aacaggtttt gaccatcgtg ggcgtacaac accggataag actgcgtgga
     5641 ttgctcgtat cccggaggca catagaccgt gagaggacgc gccgacagac cattgccggc
     5701 cacaccgaga tagcgctcca aatgacctcg ttggttcccc ccctgcacca gcgaatactc
     5761 accgtgctca tcgaaaccaa aacgtctcgc catcggatcc gctacccaat gcacaccatc
     5821 ggaaaacttg tacaacatac ggtggggccc cgtatccacg gcaaccgtga ctcgatacag
     5881 gtcctcggcc acaagcacca tcggcgttgc cgtcggatcc catccattga attcacccac
     5941 gagatgcgga tgcccccacc cttgcggacg tctgaaaaag aaggtggcat cgggcgcaca
     6001 acgtatcgga aatccgtcgg atttttcgac atcgaccaat gtttgttcga gcagcgatac
     6061 tttctccccc tcattcgatg ccgccaccag cgcggcgtcg agcgccacca aacgctcgca
     6121 atccagtgaa ccgacagcat cttccggtac atcgatcatc acgtcagcca cgacatcctc
     6181 ggtccccccc tcgggcgtgt agccacccga tgctccagca gttcccgtgg ctcccgtacc
     6241 actcgctccc cccgttcccg cctcctccaa tccttgcgtt cccccctgcc ctcccgcatc
     6301 cgattgaacc tgccccccgt ccgacgcccc tgcccccccc gctgcgggac ctggattttc
     6361 cgaatcgttt ccacacccta cggcaataca cgccgcggca atccaaccca tcgttcgtgt
     6421 catcgaaaaa ataaatgcga agcaacgaca gtacatggtc atctcctcac gagaaatcca
     6481 agcccgtcgg gactacttat gcaagtgctt gtacataaag aacgtcaatt ggtgtgtcca
     6541 tatcgcacac cgtccgaggc agggcagaag tccgccagcg cgtcccgttg ccgcgcgagc
     6601 taaagaacgc gacagccccg acggctagcc tgcttccgat gacgtagata aagagggaat
     6661 caatccatcg atgggatgct agcgcgcaat ctcgagactc cagccgttca acgttccgga
     6721 atctcccgcg gcttcatcca cgacaaccaa acgccaggtt cctttggcat cttgcccaag
     6781 gaaattcgat acgttgtagg aacgaggaac gtacgggcca tccgccgatg ctgcctcttg
     6841 caagatcgct tcgcccggca ctcccacccg ttgcaacttg atcgtgagat cgatttgctc
     6901 cggatgcgta atgtcgacga acaccttcat cgcggagatt tcaccttcat catccacgac
     6961 gatgttggag aacactcccg tgctcgaacc gtcatcgata ggtttggact ctgtgttcga
     7021 ataggttttc gcccccgacg cacaatcatt gcccacacaa tccgtgatcg atagcgtcca
     7081 gccgttgagc gtgcccgtat cgagtttcgc gttgtcgata accacgagtt tccacgttcc
     7141 cacggcatcc tggccgttga aatcactgat accaaacacc cgcttgatgt tgtgctcact
     7201 gccaccgtcc cgatcttgca ataccacctc gccaccgccc tcacgcacga gcttgatggt
     7261 gatatctccg gaatacgtat gggtaatgtc caccgcgacg gacatcgcgc tgatggttcc
     7321 acctttcgca accacgatgg agctggtcaa tcccttggga tcgttgtcag gaatggcacc
     7381 accggttccc gaaaacacct gcgtcgaccc cacacacgat ggatcctgag cgcaatccgg
     7441 atcgtcacaa tccgtcttgc catccatatc gttgtcttcg ccgtcgttgc atacctcttt
     7501 gggcaattca attttcggca gccccgaaac gtaggcattc aattcggcga cgtacttgta
     7561 cgaaatatat ccgtatccgt ctccatgggg gttatcgaca ccgaaacttc ccttgcccca
     7621 actgttcttg aagatgaaaa agcctttttc catcaagggg ttaccttgtg aatcggtcag
     7681 cagcttgcca tctccatctc gcttctgcac ctcgagggta tcatcccaac cgacgagcaa
     7741 aaacccgtgt cctgctcgtt tttccaagct cttttgttta tcgacatcat tgggataggt
     7801 aacataaccc tttcgccagt aggcattgtt ggtgggcaac ggagaaccac ggtgattcca
     7861 cgcctggtag taaaacgtcc cccccacctc gaccgccgtc tttttgctga acatatgcgc
     7921 cttgatgctg cgctcgttcg aattgatcca acgcccttga gggatgcgcc aacgcttcgc
     7981 tcccatggct gcctcaggcg gattgccatt cgtataacac tcggtcggca agctatcttc
     8041 accactgcat tccggatggt tgtagctgct ccacttggtg gattcataag gccaggcagc
     8101 ctcttccacg atgccaaatt gatagatggc tctcagattc tccgaagcac tggacccttc
     8161 cgtgttgcga aacgccttca cctcgttttt caccgaccat tgaaggaatt gctcggaaaa
     8221 atccggggtt ttgatcgtcc cctccgcaat atacaaatgc tccatatgcg ccgtggaggc
     8281 gaaaatggaa cacacgccgc gactaccctg acttttgaca ggcgactgca acttcaccaa
     8341 atcaaaatac gtcggcggat acacagcgtc agccttgccc tcctccggca gatccgcctt
     8401 cgaaggcgcc cccgccgtca aatcatcaag attgctcagc ggcgcatccc catcctcgtt
     8461 ggtcacctcc gaccgcttgt ccacgctccc ctcatccgaa gaacaggcag cccataaact
     8521 agccgaagcc aacaagatcc cggtcgatac gtacttcatc cacggtttgt cttgaagcct
     8581 gtgagtaagc atgcgtttcg tccttccttc caaacggtcc cgcttcaaag cgcccttgcc
     8641 gtcagagcct ctttgctcca cgcatccaaa gcgatcccgc tgcatttccc ttgtcaatcc
     8701 tttggccttt tgactctccg tcatggacca tccatccaaa catggcacgc actttcgtac
     8761 gatggacatt aaaaattctt cccataacca tcattgcttg tggaacaaat ccgcattatc
     8821 cgcgatctgt aaaaatatcg acccccgccc caacggccag ttcacctgcc ccatccgcca
     8881 cttctctacc ccaacccctc accccaacca ccacccccac cctcggccac cccccccccc
     8941 cccccccccc
//
LOCUS       DAOSOR010000190         8923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_7800_length_8923_cov_4.724515, whole genome shotgun sequence.
ACCESSION   DAOSOR010000190 DAOSOR010000000
VERSION     DAOSOR010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8923
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7800_length_8923_cov_4.724515"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(782..1294)
                     /locus_tag="PK710_11715"
     CDS             complement(782..1294)
                     /locus_tag="PK710_11715"
                     /inference="COORDINATES: protein motif:HMM:NF022161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 family protein"
                     /protein_id="HOT10433.1"
                     /translation="MKEVKFDHIFECGIDTYWDKVFFDEAFNHKLFHELLQFSEWKDT
                     VTKTTDEVIERTVVVRPPVGDVPAAVRKVLGDNFGYQEHGRFDRKTKRYHVDVEPNVV
                     KDKTHIHGDIWLERIDDKRSRRLAVFSLEVKIMVVGKLIEDAIAKDMTRSFERGATFT
                     NEWIRDKGIV"
     gene            1437..2117
                     /locus_tag="PK710_11720"
     CDS             1437..2117
                     /locus_tag="PK710_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HOT10434.1"
                     /translation="MIRWFQYAMLIVRKDLRIEQRTREITTTSAFFAALVSITASVAM
                     QSGPDTATRVAPAVIWIATAFAAVLAVGKSWQREREDSALDGLLVSPIHRSAIFAGKT
                     IGISIFLLVVELIVLPIVALLFSIDMLSVAPGMALIVACALPGISSTGTLFGSMTVRT
                     RARDLVFASVFFPLLLPTLIAAVAATRELFSGAPVDALQDYLLIMLLFDVVFTAGGLA
                     LFGSLIEG"
     gene            2170..2793
                     /locus_tag="PK710_11725"
     CDS             2170..2793
                     /locus_tag="PK710_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10435.1"
                     /translation="MRLAILGPADGNRAALRSRAQSALDSLNPDRIVYLGVDGIFDEV
                     LRAWALELVQEDPSDDAVWDRALRTCVAADHQTLDKFLLRERKRAKLRTITCLPHATA
                     RTVELFSGIVTILIHDKANLDEEDILPAALLVFGKGREPLIHRIGHRTFICPGPLHHP
                     RGGTTLLAEQDGVIVASMIDAKGSVVQQETVASRNQGARLTVKGASS"
     gene            2790..3359
                     /gene="nadD"
                     /locus_tag="PK710_11730"
     CDS             2790..3359
                     /gene="nadD"
                     /locus_tag="PK710_11730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOT10436.1"
                     /translation="MTTVAVFGGSFNPPHIAHVMATVFVLSTKKHIDQLLVVPCFLHP
                     FSKALAPFEDRFAMCQLAFGWIPRVTVSPIERDLGGESRTIRTLRHLQAMHPTWTLRL
                     VIGTDIVGEIPRWDSFSEIERLAPPIVLSRKGFTDSNEPLVVFPPISSTEIRQAIARG
                     QFDSVRDSVPSLVFDYIVEASLYLRSDTP"
     gene            3356..4210
                     /locus_tag="PK710_11735"
     CDS             3356..4210
                     /locus_tag="PK710_11735"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOT10437.1"
                     /translation="MTSDPFLRVHIQGAGKIGRAFATALRKARWPVRLQSARRGRPRQ
                     VDADVVLLTVRDSQLLEEANAWVPIVPSHAVVLHAAGALEPRVLSVLRSRCRGIGQLH
                     PLISVVDTRGGIRFEGAYALIAGNRKACQCATQIARVVGMHPWRNHHLDRATYHAAAG
                     LMAGGTAALFYAATHILVQAGIAPRKAQHMLVPLLRSVADNIEHVGVPRALSGVIRRG
                     DVVTLRNHIEKLSSTAPRHLSLYLAASYTQLEMARQLGDTSENNLLDVERLIAASRGS
                     EALLLRES"
     gene            complement(4331..5185)
                     /locus_tag="PK710_11740"
     CDS             complement(4331..5185)
                     /locus_tag="PK710_11740"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOT10438.1"
                     /translation="MTDDRDAELSKLRKELRIMVRRLDQCQRNRKELGYLKDKLDHVL
                     LREEEARLEIEQKRAELVELNERLSMERKRSIALLESLVPIPIAQQLRESGKTQTCIA
                     SRSTVLCADLVDYSTIAQRMAPQDIVAALDLLFSQYGSVAVRNGMEPLRSTGGLFRCV
                     GGLFASEPNEQHIARAAVNTALEIIETTRQHACYLTPQGMSWHLRVGIHVGPVVGGLV
                     GDHRLSFDIFGETVELAWALCQASEAGRVHVSREVARACDGVFDVVARSPASDKDGNP
                     VEMYWVSP"
     gene            complement(5190..7409)
                     /locus_tag="PK710_11745"
     CDS             complement(5190..7409)
                     /locus_tag="PK710_11745"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT10439.1"
                     /translation="MYARGIEPGDIIADRYLVERFIARGGMAYVVAATHIGFGDRVAV
                     KVLFPEAASQPELVQRFLLEGRVARKITSPHVVAVTDMGTLHSGTPFMIMEYLDGTDL
                     AKILQCGPLPVELATQYILQVCEALAEAHTLGIVHRDLKPANIFLTKGADGEPLIKVL
                     DFGIAKTVGGLSSMTDTHSVMGSGPYMPPEQLESARAVDPRSDIWSLGVVLFELVSGK
                     QPFYAPSLPKVFSRILNGAPDHLDTVFPQAPEGLAEVIGRCLRRNREERYADVAEFAA
                     ALCPFTNGRGRRSIATVQSVWQRASESSPAPHTLDFGTADDQTVVLPRHSPVAAANPL
                     AADSTMPSWRAEAPEKSEPFSLVVAHSDDPDTEAAVTEVLTQCLWSLRDRTPKAALVF
                     AGIDHDHQILLDRIHDQWPGLAVIGCTTDGELSSLGQFKEDSIAVALFVSDKLSFGVG
                     LGTGVALDPEAAAQQAVRQARDQLGVEPVLCITTPASLIVSGVRVLDAVKKQLGQQFP
                     VFGGTAGDQLRLEYTYQFAGTSVVKDGLPVLLIGGPLRFGAGLSSGWRPIGTSGVVTK
                     ANANVLITIGEQRAADFFVERIGKFSTPAELRAMVVSHPFAVARNDGYYLRSTFTVDL
                     DQGVVHCLGDVPEGATVQIANGSRDDVLAGSLTSIEQAIQAFDGEKIGGILVFSCAAR
                     RMLLGDRVKEEVERIQTIAPPGLPIIGFYTYGELGPLHPGGEPEFHNMTCFSLVLGE"
     gene            7628..8503
                     /locus_tag="PK710_11750"
     CDS             7628..8503
                     /locus_tag="PK710_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10440.1"
                     /translation="MKRGIFFFALVLLILISGVPAAAQSGEKRSLLLEASQAWDSADL
                     NAAAELYDQALKQGGMFPADVLVAYVRIGTVSAAKGQNNAALSAFRVAAALDPNFELP
                     SEAGPKARAIYKQARKDAEQQGGKLEITAEVPSQSAPGAEFVVVAKIDEAFAPLIVDV
                     GITVQDPSVSTSTIKPWSTKKPADTKVQFEVPGKVVMAGANLLVRVDALDAHGNRWAS
                     TQARVKVDASSDPDRFALLETKPEKKKEQNGSGGFWASPWPWVIGGALIVGGTATYFI
                     VRPNDEVLVSAPAWK"
     gene            8569..>8923
                     /locus_tag="PK710_11755"
     CDS             8569..>8923
                     /locus_tag="PK710_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT10441.1"
                     /translation="MAQGRPPSQEPSFQGTDLSTGSSDAAYGTYFLGRYRVVDEIGVG
                     GMASVHLARMDGAGGFQKWVAIKRIHPHLIEDEQFIHMFLDEARIAARISHPNVAQVF
                     DLGVHENTYWIAMEYL"
BASE COUNT         1870 a         2462 c         2573 g         2018 t
ORIGIN      
        1 ccgctcacgc cacgcccgac tgcagcttgg ggatctgcgg catcaaaagc tgtgaaacgg
       61 attgggtcaa ttgcgacggt aacgtcgcga caggttgtga gatcaacatt tccttgcatg
      121 tcgatcactg tggtgggtgc agccgtattt gcagtgcaaa ccatattcct acgcgggcat
      181 gttccggcgg acaatgcact ggagcgtgcg aaagcgggtg ggcggactgc aacaacaaca
      241 aacagaccga tggttgcgag accaatatca gcaccaatat ctcaacctgc ggaggatgca
      301 acctagcgtg cagcgcaaac catgttcctt cgcgcacttg tgtcggcggc aaatgcagtg
      361 gcgggtgcga aagcgggtgg gcagattgtg acggcaacaa acagtcgaat ggttgcgaga
      421 ccagtatcaa cagcaatgtc tcacattgcg gaggatgcaa cctatcgtgc agcgcaaacc
      481 atgttccttc gcccacatgt accggcggcc aatgcactgg cgggtgcgaa agcgggtggg
      541 aagattgcga caacaacaaa cagtcgaatg gttgcgaagc caacaccagc acgaatccca
      601 accactgcgg agggtgcggc aattcatgcc cagccggttt caactgccag ggagcgttct
      661 gccggtgcct caccagccta agctgtttaa acggtggtga atgcactagc ggtcgttgcc
      721 gctgcgacgg aaccttatgt gattacggcc aagtctgctc gccgtccggt tactgcagtt
      781 tctaaacgat tcccttatct cgaatccact cgttggtgaa cgttgccccg cgttcgaaag
      841 aacgtgtcat gtctttggcg atcgcgtctt cgatcaattt tcccaccacc atgattttga
      901 cttcgagaga aaagacagcg aggcgtcggg agcgtttgtc atcgatccgt tccaaccaaa
      961 tatccccatg gatatgggtt ttgtccttga cgacattcgg ctccacgtcc acgtggtagc
     1021 gtttggtttt tcgatcgaac ctgccgtgct cttggtaacc gaagttatct ccgagcactt
     1081 tgcgcacggc ggcgggaacg tcgcccacgg gaggtcgaac caccacggtt cgttcgatca
     1141 cctcatcggt ggttttggtg acggtatctt tccactcgga gaactgaagc agctcgtgaa
     1201 atagcttgtg gttgaaagcc tcatcgaaaa acaccttgtc ccaataggtg tcgatgccgc
     1261 actcgaagat atggtcaaac ttgacttctt tcatgctacg ttctctcctt gcttcgaatc
     1321 gctgacatct catccgagac gcttgcgaca tgccttcgtt tcgattgcct actccatgta
     1381 tcttccggtg gatacccgtt tggcgatcgt aacgtgtccc cttgcgaaac atgctcatga
     1441 tccgctggtt tcaatacgcg atgctcatcg ttcggaagga cttgcgtatc gaacagcgca
     1501 cgagggagat aacgaccacg agcgcttttt ttgccgctct cgtttccatc acggcttccg
     1561 tcgccatgca atcaggaccg gataccgcca cgcgtgtggc gccggcggtc atctggatcg
     1621 ccaccgcctt tgcggccgtc cttgcggtgg gcaaaagttg gcagcgggag cgtgaggaca
     1681 gcgcccttga cggactgctt gtctcgccta tccatcgaag cgcgatattt gctggaaaaa
     1741 caataggaat ttccattttt ctgctcgtcg tcgaactgat cgtcttgccg atcgtcgcgc
     1801 tattgttttc catcgacatg ctgtccgtcg ctcccggaat ggcactcatt gtcgcctgtg
     1861 cgctacctgg catcagttcg acgggaactc tttttggttc gatgacggtg cgcacgcgtg
     1921 cgcgggatct cgtttttgca agtgtttttt tccctctatt gcttccgacc ctcatcgctg
     1981 cggtggcggc tactcgcgag ttattttccg gtgcgcccgt ggatgctttg caggattacc
     2041 tgctcatcat gcttctattc gacgtggttt tcacggcggg ggggttggcg ctatttgggt
     2101 cgctcatcga gggatgaggt ccgttccctt ccccagtcca gcccttgtcc gtgataagtt
     2161 catcctatca tgcgcctggc catcctcggt cccgctgacg gaaaccgtgc cgcgttacgc
     2221 agccgggctc aatccgctct cgattcgctc aaccccgatc ggatcgttta cctcggggtc
     2281 gatgggattt tcgatgaagt gcttcgcgcg tgggctttgg aactcgtgca agaagaccct
     2341 tcggacgatg ccgtatggga tcgagccctg cgcacttgtg ttgctgcgga ccatcaaacc
     2401 cttgacaagt ttttgcttcg cgaaaggaag cgagcgaagt tgagaacgat cacttgtctt
     2461 ccccatgcga ccgcaaggac ggtcgagcta ttttctggaa tcgtgacgat actcatccat
     2521 gacaaggcga acctggatga agaagacatc ttgcctgccg cgctgctggt gtttggaaaa
     2581 gggcgcgaac cattgatcca ccgcattggg catcgcacgt tcatctgtcc tgggcctctt
     2641 catcatcctc gtggaggaac gacacttttg gctgagcaag acggcgttat cgtggcttcg
     2701 atgatcgatg cgaagggaag cgttgtccaa caggagactg tcgcctcacg caaccagggg
     2761 gcgcgtctca ccgtgaaagg agcgagttcg tgacgacggt ggccgttttc ggagggagtt
     2821 tcaacccacc gcatatcgcc catgtgatgg caaccgtgtt tgtgctcagc acgaaaaagc
     2881 acatcgacca actgctggtc gtcccatgct ttctccaccc attttccaaa gctcttgccc
     2941 ctttcgaaga tcgattcgcc atgtgtcagc tcgccttcgg ctggatacct cgcgtgacgg
     3001 tcagtcctat cgagcgtgat ttgggcggcg aaagtcgcac catccgaacc ctccgacacc
     3061 tccaagccat gcaccccacc tggacgttac ggctcgtcat cggtaccgat atcgtcggag
     3121 aaatcccgcg ctgggattcg ttcagcgaaa tcgagcgtct cgcccctccc attgtgctgt
     3181 ccagaaaggg tttcaccgat tcgaacgagc ctctcgtggt gttcccacca atcagcagca
     3241 ccgaaatccg ccaagccatc gcccggggtc aattcgattc ggtgcgtgat tcggtaccat
     3301 cgttggtgtt cgactatatc gtcgaagcct cgctgtactt gcgaagcgac actccatgac
     3361 atccgatcct tttctgcgcg tacacatcca aggagcggga aaaatcggcc gagccttcgc
     3421 aactgctttg cgcaaggcgc gatggcctgt ccgcttgcaa tccgcgcgtc gtgggcgccc
     3481 tcgccaagtc gatgcggatg tcgttctgct caccgtgcgg gacagccaat tgctcgagga
     3541 agccaacgcc tgggtgccga tcgtgccttc ccatgcggtc gtgttgcatg ccgccggagc
     3601 gctcgagccg cgggtgcttt cggtcttgcg cagccgatgt cgcggcatcg gacaactcca
     3661 cccactgatt tcggtcgtcg acacgcgcgg aggcatacga ttcgaagggg cttatgccct
     3721 catcgccggc aaccgaaagg catgccaatg cgccacgcaa atcgcccgcg tggtcgggat
     3781 gcatccatgg cgaaaccatc acttggatcg cgcaacgtac catgctgctg caggactcat
     3841 ggctgggggt actgccgccc tattttacgc cgcaacgcat attttggtgc aggccggaat
     3901 cgctccacga aaagcgcaac acatgctcgt tccattgctc cgaagtgttg cggacaatat
     3961 cgaacacgtg ggtgttccgc gtgcgctatc gggggtcatc cgtcgcggcg atgtcgtaac
     4021 cctgcgaaat cacattgaaa aactatcctc caccgcccca cgacacctct ctttgtatct
     4081 ggccgcctcc tacacacagc tcgagatggc gcgtcagctt ggcgatactt ccgaaaacaa
     4141 cttgttggat gtggagcgcc tgatcgcggc tagccgtggt tcggaggcgt tattgttgcg
     4201 cgaaagctga aaacgaaaca tctcgacgcc ccatcgttca tgaatgtctg gctaacgtca
     4261 ccacaagcat gattgtcgcg aggggcaggc aaaagcgctg cgacgggtgt cggaacatcg
     4321 cgcaagggct tcagggtgac acccaataca tttcgacggg gttgccatcc ttatcggaag
     4381 ctgggcttcg cgcgacgacg tcgaatacgc catcacaagc tcgagccact tcacgagaca
     4441 catggacacg acccgcttcg ctcgcctggc acaatgccca cgcaagctcg acggtctcac
     4501 cgaaaatgtc gaatgatagt ctatgatcgc caaccaatcc accgacgacg gggccgacat
     4561 gaatccccac gcgaagatgc cacgacattc cttgtggagt caggtagcaa gcgtgttggc
     4621 gggtcgtttc gatgatttcg agtgcggtgt tcacggctgc gcgggcgatg tgctgctcgt
     4681 tgggttcaga agcaaacagc cctcctacgc agcggaaaag cccaccggtc gacctgagcg
     4741 gttccatccc gtttcggacc gccaccgaac catattgtga gaacaacagg tcgagagcag
     4801 cgacgatatc ctgcggagcc atgcgttgtg cgatggtcga ataatccacg aggtccgcgc
     4861 acagcacggt ggaacgtgat gctatgcacg tttgtgtttt tccgctttct cggagttgct
     4921 gtgcgatggg aatcggaaca aggctttcca aaagagcaat ggaccgtttg cgttccatgg
     4981 agagacgctc attgagttcc acgagttcgg ctcttttttg ttcgatttcg aggcgggctt
     5041 cttcctcccg aagcagcaca tgatcgagtt tgtctttgag ataaccgagt tctttgcggt
     5101 tacgctggca ttgatcgagc cttcgcacca tgattcggag ttctttccga agtttcgaaa
     5161 gctcggcatc tcgatcgtcg gtcaccgcat cattctccga gtacgagact gaaacaggtc
     5221 atgttatgga actcgggttc tcctccggga tgcaaggggc caagctctcc gtaggtataa
     5281 aatccaatga tcggcagtcc tgggggagcg atggtttgga tacgttccac ttcttctttc
     5341 acgcgatcgc ccaggagcat tctgcgagct gcgcaggaaa atacgaggat gcccccgatt
     5401 ttttcgccat cgaaggcctg gatcgcttgt tcgatcgagg tcaaagagcc ggcaagcaca
     5461 tcgtctcgcg agccattggc gatttgaacc gttgcgcctt ccggcacatc tcccaagcaa
     5521 tgcacgacgc cttggtcgag gtcgaccgtg aatgtcgaac gcaagtaata gccatcgttt
     5581 cgtgcgacgg cgaagggatg agataccacc atggcgcgaa gttcggcagg agttgagaac
     5641 ttgccgattc tttccacaaa aaagtctgct gcccgttgct cgccgatcgt gatgagcaca
     5701 ttggcattgg ctttggtgac gacgccgctt gttccaatcg gacgccatcc cgaggatagt
     5761 ccagcgccga aacgcagggg tccaccgatg agcagcacgg gaaggccatc tttgacgacc
     5821 gaggttccgg cgaattgata ggtgtattcg aggcggagtt gatcgcccgc ggttcctcca
     5881 aatacgggaa attgttgccc aagttgtttt tttaccgcat cgagaacgcg cactccgctg
     5941 acgatcaaac ttgcgggagt ggtgatacaa agaacgggct cgacgccgag ttggtctcgc
     6001 gcttgacgga cggcttgttg cgcggctgct tctggatcca gtgctacccc tgtccccaaa
     6061 ccaacgccga agctcagctt gtcggatacg aatagggcga ccgcgatgga atcctctttg
     6121 aactgcccca gcgatgaaag ctcaccatcg gtggtgcaac cgatcacggc aaggccgggc
     6181 cattgatcat ggattcggtc gagtaggatc tgatggtcgt gatcgattcc ggcgaatacg
     6241 agagccgctt tcggagtacg gtcgcgcaac gaccagaggc attgggtgag cacttcggtc
     6301 acggcagctt ccgtatccgg atcgtcgcta tgggccacca ccaacgaaaa aggttccgac
     6361 ttttccggag cctctgctct ccaactcggc atcgtactat cggcggcaag ggggttagcc
     6421 gccgccactg ggctatgacg tggcaaaacg accgtttgat cgtcggcggt accaaagtcg
     6481 agcgtgtggg gcgcgggact cgactcgctg gctctttgcc acacggattg gacagtagcg
     6541 atggaacgtc gtcctcgccc attggtaaaa gggcagaggg cggcggcgaa ttctgctaca
     6601 tccgcgtagc gttcttcacg atttctacgc aggcaacgtc cgatgacctc ggcaagacct
     6661 tcgggtgctt gggggaacac ggtatcgagg tggtcgggcg ctccgttgag aatgcgagag
     6721 aagactttgg gcaaggaagg ggcataaaag ggttgttttc cgctgacgag ttcgaacagc
     6781 acgacaccga gcgaccagat atcgcttcgc gggtcgacgg ctctagcgga ctcgagttgt
     6841 tcgggtggca tatagggacc ggagcccatg acggaatggg tatcggtcat cgaagagagt
     6901 ccgcccacgg tttttgcgat tccgaaatcc agcactttga tgaggggttc cccatcggcg
     6961 cctttggtaa gaaagatgtt ggcgggtttc agatctcgat gaacgatacc caaggtgtgg
     7021 gcttcggcga gtgcttcaca aacttgcagg atgtattgtg tggcaagttc gacgggcagg
     7081 ggaccgcact gaaggatttt ggcgaggtcg gtgccgtcga gatattccat aatcatgaaa
     7141 ggggtgccgg aatgcagggt tcccatgtcg gtgacggcga caacgtgggg gctggtaatt
     7201 tttctggcta cacgtccctc gagcaaaaaa cgttgcacga gttcgggttg ggaggctgct
     7261 tcgggaaaca ggactttgac ggccacgcgg tcgccgaagc cgatatgggt agcagccacg
     7321 acgtaggcca taccgccacg ggcgatgaat cgttcgacaa gatagcgatc tgcgataatg
     7381 tcccctggtt cgattcctcg tgcgtacatg ggcgctcctt tgaaagaggg ttctaagacc
     7441 gtgggagagg aatcggcagt ggggccaagg tgggagaatc tacgccaacg ttgtagaagt
     7501 ccctgtcagc gcgttgcttt tgaagtaaaa atcaagcatg attccgtggg tggcgacctt
     7561 gttctgggat atggattcat tgccccgtct gcctgcgcca ggggcccagt gccatgagga
     7621 ataacacgtg aaacgaggaa tttttttctt tgccctggtg ctgcttatcc taatttccgg
     7681 ggttcctgcc gcggcgcaat cgggtgagaa acggtcacta ttgctggagg cttcccaggc
     7741 atgggattca gcggacttga acgccgccgc ggaattatat gatcaagcct tgaaacaagg
     7801 gggaatgttc cctgcggacg tgctcgtagc ctatgtgcgc atcggtacgg tgagcgcggc
     7861 caaagggcag aacaacgcgg cgcttagtgc attccgtgtg gcggcggcct tggatccgaa
     7921 cttcgagctt ccttccgagg cggggcccaa agcaagggcc atctacaaac aagcgcgaaa
     7981 agatgccgaa caacaaggag gaaaactcga aatcaccgcg gaagttccct cccaaagtgc
     8041 tcccggcgcg gagttcgtcg tggtggcaaa aatcgatgaa gcctttgctc cactcatcgt
     8101 cgacgtaggc atcacggtac aagatccttc cgtatccaca tcgaccatca agccttggag
     8161 cacaaaaaaa ccggcggata cgaaagtgca attcgaggtt ccgggcaagg tggtgatggc
     8221 gggggccaac ttactcgtac gggtcgacgc gctcgatgcg catggaaacc gttgggcctc
     8281 tacgcaagcc cgggtgaaag tcgacgcctc ttcggacccc gaccgctttg cattgcttga
     8341 aaccaaacct gaaaaaaaga aggagcagaa tggttccggg gggttttggg cttcgccttg
     8401 gccgtgggtt attggtggtg cgctcattgt aggaggcacg gctacctatt tcatcgttcg
     8461 tcctaacgac gaagtattgg tcagcgcacc cgcatggaag taatctcctg aacctgtaga
     8521 atgtcgcagc ggtctttcgc tcccgtaagc gatatgtcac aaaatacaat ggctcaaggt
     8581 cgccccccat ctcaggaacc ttcttttcag gggaccgatc taagcacggg ctcctcggat
     8641 gctgcttatg gcacctactt tctcgggcgc tatcgggtgg ttgacgagat tggggtgggg
     8701 ggtatggcca gtgtgcatct ggcccgtatg gacggagcgg gggggtttca gaagtgggtg
     8761 gcgatcaaac ggatccatcc ccatctcatc gaggacgagc aattcattca catgttcctc
     8821 gatgaggctc gcatcgcggc gcgcatttcg caccccaacg tagcgcaggt gtttgatctc
     8881 ggtgtccatg aaaatacgta ttggattgcg atggaatacc tcc
//
LOCUS       DAOSOR010000191         8871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_7854_length_8871_cov_4.367287, whole genome shotgun sequence.
ACCESSION   DAOSOR010000191 DAOSOR010000000
VERSION     DAOSOR010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8871
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7854_length_8871_cov_4.367287"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..600)
                     /locus_tag="PK710_11760"
     CDS             complement(88..600)
                     /locus_tag="PK710_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10442.1"
                     /translation="MILRQLIQVSIIAVGALGCGYRSTLPSAAPSHWCVQTHLAPALY
                     PQASAALVFGVREALAQRGWLANCSNARVLRVQVLDVRFETEGLIAQSSAASARAMRV
                     VVSAAADIDRSPADRELMVTESYEVASMDSPLAQEQANDIGVVTAARQAGHRLVQKLA
                     HEPSVGTSSP"
     gene            complement(607..3087)
                     /gene="leuS"
                     /locus_tag="PK710_11765"
     CDS             complement(607..3087)
                     /gene="leuS"
                     /locus_tag="PK710_11765"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOT10443.1"
                     /translation="MSTTPKNQEDRYSPAFIEPKWQTYWDEHHTFRSVRRPGHPKLYV
                     LDMFPYPSGAGLHVGHPEGYTATDIVARHARMKGVDVLHPMGWDAFGLPAEQYAIQTG
                     THPSQKTKENIENFRRQLKMLGFSYDWEREIDTTQSDYVRWTQWIFLQIFHQGLAVQS
                     EIPVNWCSELGTVLANEEVIDGKSERGGHPVQRIPLRQWSLKITEYADRLVHDLECVD
                     WPDTKLKQINWIGRSEGCEIEFAVDQYPSSLRVFTTRPDTLFGATYVVIAPEHPLVET
                     ISAASCKDAVRAYVQAASARSDLDRVATSKDKTGVATGAFARHPLTGEKIPIWVADYV
                     LGSYGTGAVMAVPGHDERDFVFAQKHDLPIIEVVSPDGSLSESLLSPFIEDGISVRSG
                     AFDGLPTPEMKRRIIDHLERHHLGKASVSYKLRDWVFSRQRYWGEPFPIYFPVEVAND
                     GDPRKGDPHTIRYDQPIAVDESELPVNLPDLHDFRPSNDPRGPLARALDWVFFQRDGK
                     WFARETNTMPQWAGSCWYYLRFLDPRNDKRPFSEQSYDDWMPVDLYVGGSEHAVLHLL
                     YARFWHKVLFDIGLVKHREPFHKLVHQGMILGENNEKMSKSRGNVINPDDVVREFGAD
                     ALRAYEMFMGPLEQVKPWKTSGIQGVRRFLERVYKLCLSSEAEAKADEATLRLVHKTI
                     KKVGDDIEAMHFNTAVSALMILSNHLSTLQPVPMDAARTLVLLLSPITPHLAEELWQR
                     LGGTPSVSLQPWPTYDEALVREDVVEIGVQVNGKVRGRIALARDASEDEALALALEQE
                     NVRAHVPDGSVRKIIYRPAQILNIIVAK"
     gene            complement(3134..3619)
                     /locus_tag="PK710_11770"
     CDS             complement(3134..3619)
                     /locus_tag="PK710_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10444.1"
                     /translation="MNAILREMQEDIPLVRKPFVTIGYDEKPLGDDVSPAESYAPTCI
                     DDANAAPEIIVSETRPGLETMAVIDEELLRDARQALQRQSSETLRSAEVLELLTFVIL
                     DKSLPLSASMEERRTFVAERLWHRLPAGGINAVRRIDIRPADETALMMRVWCAVPKLT
                     P"
     gene            complement(3828..4151)
                     /locus_tag="PK710_11775"
     CDS             complement(3828..4151)
                     /locus_tag="PK710_11775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT10445.1"
                     /translation="MPTYEYKCAACGHEWEEEQRIVDAPLDTCPACKEKTAHRQISAG
                     NFILKGGGWYADLYSSPSKKGGNGGASKSSQPSHSSQPSKEPATPSSSASSPAANTAD
                     SSKTS"
     gene            4363..7956
                     /gene="smc"
                     /locus_tag="PK710_11780"
     CDS             4363..7956
                     /gene="smc"
                     /locus_tag="PK710_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOT10446.1"
                     /translation="MYIKKLEISGFKSFVDRTVVHFDHDVTGIVGPNGCGKSNIVDAI
                     RWCMGEQSPRQLRGKSMGDVIFSGSESRKPFGFAEVTITFSNSDPEIARTLPIEYRDY
                     PEIAVTRRLYRDGTSEYLVNKTQVRLKDLNDLFLGTGVGSKAYSIIEQGKIGLIVSAK
                     PEDRRLLIEEAAGITKYKHRKKQAEIKMDLTRQNLLRVGDIVTEIEHNMASLKRQAAK
                     AKRYLDYRREHDDLVLHEASHRLLAILAVEKVERGAVEQTRERVEQERTELGTRQSVL
                     EAGRAECVLLENQVDKDQNAAFLADNEVRTCDAKAQRSMDRLGVLRERQQAAAQERQR
                     LRIEAERLQKEQAAVRENLSKFEQTEAEHAERLRIEEDKLRLREHELREVETRTSWLR
                     TTMAKSSASAAACQERLSAFDQRFEDMRLRRERLIIDSERFREDLKEVQDRRASLAQS
                     VVTLDQDLQSATQKVSDYSTQLQGLRERMVASDRHVESVKSELSQSRGQLRALEEVHA
                     RLEGVGSGTRSLMDTRDPCIVGMVADLIEPHEDCLVPFAALMGDRLQTVVVRDMDRAT
                     VLLRELAARKAGRASVIFCSPPFVATAWDVKAAGVLGPLLHRLRYPREYEALMHALVG
                     DALLVENTDTARSLRSQGVQATLVTVEGTVYHRDGRISGGAGDEVAAALVQQKLRLRE
                     MRQQVVEQEKRADTALASHESLRKEILELGDQLERQRSAERDLQLEKVTLDHDLRRFD
                     DQMTTVNSRLGEIEKEVEALDMRLDDTALERDEVERTLLRSQAEAKAVAEQLEVAEQE
                     SRNFTEQVSLQKETCTERKIEFVRIREQASSMRATQERLDRSIHELLDRDHGLEQEIT
                     SSSVEQGHVAAELYSLKERMVEASIAAKASHAEFERRKQSLDAARSALAVHETEIKEL
                     RDRLDQDVEKQRRHEMALQKLELEKQHLFEAIAEKFRGLRLATVVGDYHKRPRPDDEH
                     RARIEELGNLIDRMGPVNLDAVEDYDKASERYTFYTTQKQDLEKALSDLEQAIQQMNR
                     ESRRLFRNTFDSVNERFQEIFPRMFQGGKATLVLTNPEDLLETGVEIRAQPPGKKVGS
                     IELLSGGEKALTAVSLIFAIFQHKPSPFCVLDEVDAPLDEANVTRFNDLVRSMTDRSQ
                     FILITHIKRTMQSVDVLYGVTMQEPGVSRLVSVKVNPAAQPRWVAGAEAVA"
     gene            7985..8500
                     /locus_tag="PK710_11785"
     CDS             7985..8500
                     /locus_tag="PK710_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10447.1"
                     /translation="MMIGIRRLRWLGTCRRHAACLVTWLVAGLTVLTMAGCGDDDETK
                     AVIPLGPPSLSVVFPSDGACVMFHDDEVPAVRVRIEAQNWLLRPIGYCGETYSQCGHA
                     VFFVDGNEMIRSASLVTDVPFEKLSSPTGKHHLRVELRNDDDVVVLDRMKEPLEAEVS
                     IEAVEAGQACP"
     gene            complement(8517..>8871)
                     /locus_tag="PK710_11790"
     CDS             complement(8517..>8871)
                     /locus_tag="PK710_11790"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOT10448.1"
                     /translation="TLVSTGEAGLGDPEKSKSTKRGIFRIDRKHLTATMSSSEVGEEF
                     ELNDIPFVQYFEGGYALHAAYWHDDFGVPKSHGCINLSPDDARRLFFWTEPRIPPDWH
                     SVQRSLTGSVVFVHP"
BASE COUNT         2051 a         2256 c         2667 g         1897 t
ORIGIN      
        1 aatcatggtg ttagcacggt atcacccaac ggcactcagg ccaacgagag cataatttat
       61 tcgtaaatac aaatagttac aaatgcatca aggagacgaa gtgccgacgg aaggctcgtg
      121 ggcaagcttt tgaacgagcc tatggccggc ctgtctcgcc gctgtgacca ccccaatatc
      181 gttggcttgt tcctgcgcaa gcggcgaatc catcgacgcc acctcgtatg attcggtcac
      241 catcagttcc ctatccgccg gtgaacgatc gatatccgca gcggcggaga cgacaacacg
      301 catggcgcgg gcggatgccg ccgaagattg tgcaatcagc ccttccgttt cgaatcgaac
      361 atcaaggact tggactcgaa gcacccgagc gttgctacaa tttgccaacc atcctcgttg
      421 cgccagcgcc tctcgcacac cgaaaacgag tgcggcagat gcttgggggt acaaagccgg
      481 agccaaatgt gtctgtacac accagtgact tggcgctgcg gacgggagag tcgatcgata
      541 gccacaaccg agcgccccca ccgcgatgat cgacacctgg atgagctggc gaaggatcac
      601 ggctcgctac tttgccacga tgatattgag aatttgcgcg ggtcgataga tgattttgcg
      661 cacggagcca tcggggacat gagcacgaac gttttcttgt tccagggcga gagcgagggc
      721 ttcatcttcg gaagcatcac gcgccaaagc gattcttcct ctcactttcc cgttcacttg
      781 caccccgatt tccacgacat cttcgcgaac gagggcttca tcgtaggtag gccacggttg
      841 taaggatacc gatggcgtgc cgccaagacg ttgccacagt tcttccgcaa gatgaggtgt
      901 gatgggcgac aacagaagga ccagtgttcg cgccgcatcc atgggcaccg gttgcagcgt
      961 agaaaggtga ttcgacagaa tcatcaaggc gctgacagca gtattgaagt gcatcgcttc
     1021 aatgtcatcg cccactttct tgatggtttt atgcacgagc cgcagagttg cctcatcggc
     1081 cttcgcctcc gcttcggaag acaagcacag cttataaacg cgctcgagaa aacgtcgcac
     1141 cccttgaatt ccacttgtct tccagggttt gacctgttcg aggggcccca tgaacatctc
     1201 gtaggcgcga agggcatcag cgccgaattc gcgaaccaca tcgtccgggt tgatgacatt
     1261 tcctcgagat ttcgacattt tttcattgtt ttccccaagg atcatccctt gatggacgag
     1321 cttgtgaaac ggctctcgat gcttgaccaa gccgatatcg aacagcacct tatgccagaa
     1381 ccgcgcatat aaaagatgca gcacggcatg ctcgctaccg cctacataca gatcgacggg
     1441 catccaatcg tcgtaggatt gctctgaaaa cggccgcttg tcgttacgcg ggtccaggaa
     1501 acgaaggtag taccagcacg aaccagccca ttggggcatg gtattggtct cgcgagcaaa
     1561 ccactttcca tcgcgttgaa agaacaccca atcgagggcg cgagccagag gcccccttgg
     1621 atcgttgctt ggacggaagt cgtgaaggtc tggcaaattc acgggtagtt cggattcatc
     1681 gacggcgatg ggttggtcgt aacggatggt atgggggtca cctttgcggg gatcaccgtc
     1741 atttgcgacc tcgacgggaa aatagatggg aaagggttcg ccccaatagc gttgccggga
     1801 aaacacccaa tcacgaagtt tatagctgac ggacgctttt ccgagatggt gtcgttccag
     1861 gtgatcgatg atgcggcgtt tcatttcggg ggtcggcaaa ccatcgaacg ctccggaacg
     1921 cacggagatc ccatcttcga taaacgggct taatagcgat tcgctcaggc ttccatcggg
     1981 actgacgact tcgatgatcg gaagatcgtg tttttgcgcg aagacaaagt cgcgctcatc
     2041 gtgcccgggc acggccatga cggcgccggt tccataggaa ccgagtacat agtccgcaac
     2101 ccagatcgga attttctctc cggttaacgg atgacgtgca aatgcgccgg tggctacacc
     2161 tgttttgtct ttggaagtgg ccactcgatc cagatcgctt cgcgcggaag cggcctggac
     2221 ataggctcga acggcatctt tgcacgaggc ggcagagatg gtttccacga gggggtgttc
     2281 gggggcaatg accacatagg ttgcgccgaa tagggtatcg gggcgcgtgg tgaagacgcg
     2341 aagggagctt ggatattggt cgacggcgaa ctcgatctcg catccttcac tgcgaccgat
     2401 ccagttgatt tgcttgagtt tggtgtcggg ccaatccacg cattcgagat catggaccaa
     2461 tcggtctgca tactccgtga tcttgagcga ccattgtcga aggggaattc gctgcacagg
     2521 atggccgcca cgttcgcttt ttccatcgat gacttcttca ttggcaagga cagtgccgag
     2581 ttcggagcac caattgactg ggatctcgga ttggacggca aggccttgat ggaagatttg
     2641 tagaaaaatc cattgcgtcc aacgaacata atccgattgg gtggtgtcga tttcccgttc
     2701 ccaatcgtaa gaaaatccga gcattttgag ttgtcgtcgg aagttctcga tgttttcttt
     2761 cgttttttgg gacggatggg ttccggtttg gatggcgtat tgttccgcgg gcaggccgaa
     2821 agcgtcccaa cccatgggat gcagaacatc gacgcctttc attcgggcgt gtctggccac
     2881 gatatcggtt gcggtatacc cttccgggtg acccacgtgc agacccgcac ccgaggggta
     2941 gggaaacatg tcgagcacgt agagtttggg atgcccgggt cgacgcaccg agcgaaacgt
     3001 atggtgttca tcccaatacg tttgccactt gggttcgatg aaagcggggc tgtagcggtc
     3061 ttcttgattc ttcggcgtag ttgacatgag cgcccaccgt gtagcacacg gaccgaggac
     3121 agggcgcgga aagttaaggt gtcagttttg gcacagcaca ccacacgcgc atcatcaacg
     3181 ccgtttcatc cgcaggacga atgtcgattc ttcgaacggc attgattcca ccggcaggca
     3241 gtcgatgcca aagacgttcc gcgacgaacg tacgtcgttc ttccatcgaa gcagacaggg
     3301 gcaaggattt gtcgagaatg acgaaggtca gcaactccaa gacttcagca cttctgagcg
     3361 tttcggaaga ttgccgttgc agggcttgcc gtgcatcacg cagcagttct tcgtcgatca
     3421 ccgccatggt ttccaaccct ggacgagttt cggagacgat gatttcggga gccgcgtttg
     3481 catcgtcgat acacgtgggg gcataggatt ccgcaggaga cacatcgtcg ccgagtggtt
     3541 tttcgtcata tccgatggtg acgaaaggct ttcggaccag cggaatatct tcctgcattt
     3601 cgcgaagaat cgcgttcaag gtggcacgac cgacaggtcg ttgctcgaga gatatttctg
     3661 gtggcggggg tttggatgaa cgcccggacc gacctttccc aagttttcgg gagcgacccc
     3721 ctggattttt tgggatcatg agtattggag gcgatactag cactattctt cgcctggggt
     3781 acacgttccg aaaggcgcat cgaaacggag cctccgcttg cttaacccta ggatgttttt
     3841 gaggaatcgg cggtgttggc cgctggagaa ctagcggagg atgaaggggt tgcaggctcc
     3901 ttggaaggtt gggaagaatg ggaaggctgg gaagacttgg aggcaccccc atttcctccc
     3961 tttttcgaag ggctcgaata caagtccgcg taccagccac cgcctttgag gatgaaattg
     4021 ccagccgaaa tttgtcgatg ggctgttttt tccttacagg cggggcaagt atcgagcggg
     4081 gcatcgacga tccgttgttc ttcctcccat tcgtggccgc aggcggcgca cttatactcg
     4141 taagtaggca tagtcagaca cctttcctgc acggcgtagc atcaatccgc cgcgacgcac
     4201 gcttcccgat gcgtcttgcg aatcattctg tcaagagtcc atccactgtc tgaacgttcg
     4261 aaagccttgc gctccgaacg cagtacaggt agcaggaggg gcaggaccat atatgtcacg
     4321 tcatcaaatg acacccacgg tccgttcacg tcagtccaca tcatgtatat caagaagctc
     4381 gaaatctctg gcttcaagtc attcgtcgac cgcacggtgg tccacttcga ccacgatgtg
     4441 acggggatcg tcggacccaa cggatgcggg aagtcaaaca tcgtggatgc catccgttgg
     4501 tgtatgggag agcaatcgcc tcgtcaattg cgaggcaaat cgatgggcga cgtcattttt
     4561 tcgggttcgg aaagccgaaa accattcggt ttcgccgaag tcaccatcac gttttccaat
     4621 tcggatccgg aaatcgcccg aacgctgccc atcgaatacc gggactatcc cgagatcgcg
     4681 gtcacccgca ggctctatcg cgacggcacg agcgagtact tggtaaacaa gacgcaagtg
     4741 cgactcaagg atctgaatga tctatttttg ggaaccggcg tggggagcaa ggcgtattcg
     4801 atcatcgagc aggggaaaat cgggttgatt gtctcggcaa aacctgagga tagacgtttg
     4861 ctcatcgagg aagcggctgg catcaccaag tacaagcatc gaaaaaagca agcggagatc
     4921 aagatggatc tcacgcgcca gaacctgctg cgggtaggag atatcgtcac cgagatcgag
     4981 cacaacatgg cgtcgttgaa gcgtcaggcg gccaaagcca agcggtatct cgactaccgc
     5041 cgagagcacg acgatcttgt gttgcatgag gcttcacacc gattgcttgc cattttggcg
     5101 gtggaaaaag tggagcgggg tgcagtcgaa cagacccgtg agcgggtcga gcaagagcga
     5161 acggaactcg gcacgcgcca atcggtgctg gaagcgggcc gagcggaatg cgtcctcttg
     5221 gaaaaccagg tcgacaagga tcaaaacgct gcatttctcg ctgataacga agtacgaacc
     5281 tgcgatgcca aagcgcagcg ctcgatggac cggctggggg tgcttcgaga acgccaacaa
     5341 gccgctgccc aggaacggca gcgactacga atcgaggcag aacgacttca aaaagagcaa
     5401 gcggctgtcc gcgaaaacct gtcgaaattc gaacagacgg aggcggagca tgctgagcga
     5461 ttgcgcatcg aggaagacaa gcttcgcttg cgcgaacacg aattgcgcga agtggaaacg
     5521 cgaacgagct ggctccgaac gacaatggcg aaaagcagcg cctcggccgc cgcttgccaa
     5581 gagcgtcttt ccgccttcga ccagcgtttc gaagatatgc gcttgcgtcg agaaagactg
     5641 atcatcgatt ccgaacgttt tcgcgaagac ttgaaggaag tgcaggaccg gcgtgcatcg
     5701 ctcgctcaat ccgtcgtcac actggatcaa gacctgcaat cggcgacaca aaaggtgagt
     5761 gactattcca cgcaactgca aggcttgcga gagcgtatgg tagccagtga ccggcatgtg
     5821 gagtcggtaa aaagtgaatt gagtcagagt cgagggcaac ttcgcgcttt ggaggaggtg
     5881 catgcgcgtc tcgaaggcgt gggatcggga acaagatcgc tcatggatac ccgtgatcca
     5941 tgcatcgtgg gaatggtggc ggatctcatc gaaccgcacg aagattgtct tgttccgttc
     6001 gcggcgttga tgggagacag actccagacc gttgtcgtac gtgatatgga ccgagccacc
     6061 gtgctgcttc gggaacttgc ggcacgcaaa gcgggtcgag cgagcgtcat cttttgtagt
     6121 ccaccattcg tcgcgacggc gtgggatgtg aaagccgcgg gggtccttgg accgctgctg
     6181 catcggttgc gctatccccg cgaatatgag gctcttatgc acgccttggt gggcgacgcc
     6241 ttattggttg aaaataccga cacggcgcga agccttcgat cacaaggtgt gcaagcaacg
     6301 ctcgtcaccg tggagggaac ggtatatcac cgcgatggcc ggataagtgg cggggctggg
     6361 gatgaggtcg cggcggccct cgtccagcag aagctgcggc ttcgtgagat gcgccagcag
     6421 gttgtcgaac aggaaaagcg cgcggatact gccttggcat cgcatgaatc acttcgaaaa
     6481 gagatactcg agttggggga ccagctcgaa cggcagcgaa gcgcagagcg cgatttgcag
     6541 ctcgaaaagg taacactcga ccatgatctt cggcgtttcg acgatcagat gaccacggtg
     6601 aactcgagac ttggtgagat tgaaaaggaa gtggaggcgc tcgacatgcg tctcgacgat
     6661 accgcgctcg agcgtgatga ggtggaacga accttgctcc gatcgcaggc tgaggcgaaa
     6721 gctgtcgcgg aacaactcga ggtggcggag caggaatctc gcaactttac tgaacaagta
     6781 tcgctacaga aagagacctg tacggagcgg aaaatcgagt ttgtgcgcat tcgagagcaa
     6841 gcgagttcga tgcgtgcaac ccaggaaaga ctggatcgtt ccatccatga actgctcgat
     6901 cgagaccatg ggctcgagca agagatcacc tcctcgtccg tagagcaagg gcatgtcgcc
     6961 gcggaattgt attcgttgaa agaacgaatg gtcgaagcat cgatcgcggc aaaagcctcg
     7021 catgcagaat tcgaacgacg caaacaatcg ttggatgcgg cacgcagcgc gctggcggtt
     7081 catgaaaccg aaatcaagga attgcgtgat cggttggatc aagacgtgga aaagcagcgc
     7141 cgtcatgaga tggcgcttca gaagttggaa ctcgaaaaac agcacctatt tgaggcgatt
     7201 gcggagaagt ttcgaggtct tcgtttggca acggtagtgg gggattatca caagcgtccg
     7261 cgcccggacg acgaacatcg ggcacgcatc gaggagctgg gaaacctgat tgatcgaatg
     7321 ggtccggtga atctcgatgc ggtagaagac tatgacaagg caagtgagcg atacacgttt
     7381 tacacgacgc aaaaacagga tctcgaaaag gcgctatcgg acttggagca ggcaatccag
     7441 caaatgaacc gggagagtcg tcggttgttt cgcaacacct tcgatagtgt caacgagcga
     7501 tttcaagaga tttttccgcg tatgttccaa ggcggcaaag ccaccttggt gctgacgaat
     7561 ccggaggatt tgctcgaaac aggggtagag atacgagcgc aaccgccggg gaagaaagtg
     7621 ggctcgatag aattgctcag tggtggcgag aaggcgctga cggcggtatc ccttattttc
     7681 gctattttcc agcataaacc atcacctttt tgcgtgctcg acgaggtgga cgcgccgctg
     7741 gacgaggcca atgtgactcg gttcaacgat ctggtgcgtt cgatgacgga ccgttctcaa
     7801 ttcatattga tcacgcatat caaaaggacg atgcagtcgg tggatgtatt gtatggggtc
     7861 acgatgcaag agcccggtgt atctcgtttg gtgagtgtga aggtgaaccc ggcagcacag
     7921 ccgcgatggg tggcgggtgc ggaggcagtg gcgtagcgtt cgatgggaga ttagtctgtt
     7981 ggccgtgatg attggaattc gtcgtttgcg ttggcttggc acctgtcgaa gacacgctgc
     8041 ttgcttggtt acgtggctcg tcgcggggct gacagtgttg accatggcgg ggtgcgggga
     8101 tgacgatgaa accaaggcgg taatcccact cggtccccct tccctatcgg ttgtttttcc
     8161 ttcggacggg gcatgtgtca tgtttcacga tgacgaagtt ccggcggtcc gggttcgcat
     8221 cgaagcgcag aactggctat tgcgccccat cggctattgc ggcgaaacgt attcacagtg
     8281 cgggcatgcg gtattttttg tcgacggaaa cgaaatgatt cgcagcgctt ccctcgttac
     8341 cgacgttccg ttcgagaagc tgtcgtcgcc aacgggaaag catcatcttc gggtggaact
     8401 acgcaacgac gacgacgttg tcgtcttgga tcgaatgaaa gagcctttgg aggctgaggt
     8461 atcgatagaa gcggtggaag cggggcaggc gtgtccctga tgtggattgg gccgggtcaa
     8521 gggtgaacga aaacgacgga tccggtgagc gagcgttgta ccgagtgcca atccgggggg
     8581 attcgaggct cagtccagaa aaatagccgt ctggcgtcat ctggcgagag gttgatgcaa
     8641 ccgtggcttt tgggcacccc gaagtcatca tgccaatagg cggcgtggag tgcgtagccg
     8701 ccttcgaagt actgcacgaa ggggatatca ttgagttcga actcttcgcc gacttcggag
     8761 gaggacatgg tggcagtcaa gtgttttcga tcgatccgaa aaattccacg tttggtcgat
     8821 ttagactttt ccggatctcc gagcccggct tctccggtcg aaacgagggt c
//
LOCUS       DAOSOR010000192         8830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_7899_length_8830_cov_3.927635, whole genome shotgun sequence.
ACCESSION   DAOSOR010000192 DAOSOR010000000
VERSION     DAOSOR010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8830
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7899_length_8830_cov_3.927635"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="PK710_11795"
     CDS             <1..1034
                     /locus_tag="PK710_11795"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10449.1"
                     /translation="LFSMPNRLVATFSLFGILALLPNCQLFVQSDLDKVYCEQEGVIG
                     PPACPSGASCQRGQCVMCSNAETCGDGIDNNCNGLRDEGCEDAGSGDGDAPWDADDAP
                     ATQDVFEPPEPNLDGRVTTGLQVLYAFMEGQGTTVKDYAPNLDPIDLKIESFDHVKWK
                     PGGGLHIAGATRIAASNTQRLIDACRKQNAITVEAWIEPEPNEPQSFAPFASLSFSSS
                     LCNFTLAQQNHQFAFRLRTTNSDNQGMPTLLSETNAVTDTLTHLVVVRNQSGLVRFVV
                     DGVEKGKQQRNGSFSNWGSNAMLTVGNAFSEDPTWLGTLYLIAVYCHPLMDSDIAKNY
                     AVGKPTARQ"
     gene            complement(1046..2347)
                     /locus_tag="PK710_11800"
     CDS             complement(1046..2347)
                     /locus_tag="PK710_11800"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT10450.1"
                     /translation="MVEEAEQAVPNYVSSHLPQSPLISKGYVVADKYELVAMIGQGGM
                     GTVWLARHIHLERKVAVKFISIIHIESEEMLRRFDGEAKAVASLHSRHVVQVIDNGEL
                     EDGTPYMVMEYLEGESLHARIERQGAVSIDDAERIFRHVGMALSVAHDKGIIHRDIKP
                     ENIFLTISPEESDYVAKVLDFGVAKMTQARYASHARTRTGTIVGTPMFMSPEQVRGLK
                     TVDHRADVYSMGALAFTMLTGSSVFDGKTFGELLFQICSEPLPKLTDRVPGLPVAVDT
                     WFETSCARSPADRFQSVQEQVKAFSQALGKSGRASFSDDEIKLDDGRLSRSSPTTEKK
                     PSEREAAPETDTEPKTPSLLAEANSSEPQERKSTLSSPAVEAPSVRKLSSPASQPSRF
                     GMPLRIVAALLLIALVAAGSFFLGQRTGTQPKDSSTTPASS"
     gene            2661..3434
                     /locus_tag="PK710_11805"
     CDS             2661..3434
                     /locus_tag="PK710_11805"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT10451.1"
                     /translation="MSESIFDHTAFNARVFHPREDEGSAPPGAEDLIIPVEHDIRLHV
                     RVYPAPSAQRAVLLFHGNGELVADYDFVAHHYLRMNTTLAVVDYRGYGRSEGDPRFRT
                     MIEDAPKVVEALQPVLSRETRLPLVVLGRSLGSACAAEIAKRSPDGVVGLVFDSAFSN
                     LKAFALRRGRQYDVPLSEQDYEAFCPLRKLASCHLPILIMHGAQDTLLAPTEAQMLFD
                     ATASARKRIVFIEEYGHNDLFYAPAYWQNLTSFLEEIAS"
     gene            3431..4075
                     /locus_tag="PK710_11810"
     CDS             3431..4075
                     /locus_tag="PK710_11810"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOT10452.1"
                     /translation="MITPKALIFDLDGLLVDSEPIWFEVEGGFLAEFGHIWTIEEAHA
                     CMGQGTPNTLRIWKQRYGIPVDIPRDTERIIDRMIARAHEMPLKTGAIQLLEHARQQR
                     CPMAVASSSPQKLISSVLAAKQIDSFFRAIVSGQDVPCSKPAPDVFLRAAECLDIDPA
                     YCVVLEDTLAGTRAGIAAGMRVAAVPSSNPHEIASLATWVLRDLKQAIDVLFGS"
     gene            complement(4138..4611)
                     /locus_tag="PK710_11815"
     CDS             complement(4138..4611)
                     /locus_tag="PK710_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10453.1"
                     /translation="MNEVNDRCLNCGASLTPEDYMLPACRFCGTAYPHHLEAAKLAGQ
                     VHAVMRGVVGPMPPMPQQGGGSAPVVVVPGMQPIAHASSLYVHQSVSLGASPPATGGP
                     VASGGSSPAPWGDPRFDHRNHSRLSMWIWVTVIVFLLLAFLGFLAGFFFVFGMLR"
     gene            complement(4608..5498)
                     /locus_tag="PK710_11820"
     CDS             complement(4608..5498)
                     /locus_tag="PK710_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10454.1"
                     /translation="MNDPYSPIQGISLERYAELSAEISDHLNDTEAQMRIVESLGVSR
                     ADWQAAVAGWTARMQDMSLMGQVATRYMSLYQAALSKKKGSVDVSYDDWVALNAAVQA
                     YGIEGALRAYGIDMTTWTMIAGTWNSRIGQNMMAYQSFHPSVTAEAQRIRSGAAHRPV
                     TFLRQAGGSGSSNAATPAATPQAATPQAAAQQYQNQMVGLQVQADVAQAVAQANAQAA
                     AAYGDVSKNVGFLGRGVLGAMGMGAIAKGIGPGMTVMVAWSNGNRYPGQVVQVSGGKV
                     LVAFQNGQQMWVPENAVSAQ"
     gene            complement(5708..6850)
                     /locus_tag="PK710_11825"
     CDS             complement(5708..6850)
                     /locus_tag="PK710_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT10455.1"
                     /translation="MLDFTLSEEQKALTDMAHRFAKEKIVPIAAQCDEKSEFPVDVFQ
                     EAWELGLVNPTIPESYGGPGLHELDNCLIAEELAWGCIGVETTIMANTLGLTPILLAG
                     SEEQKKKYLGMLASEPKFCAYATTEAGAGSDVAALKTKFTKVGDDYVLNGEKAWITNA
                     NLASFFVIFATIDPALKHKGIAAFIVDRDTPGLHPGKHEDKLGQRASDTATVTLEDVR
                     VPKQNLLAPEGKGFKLAMETFNQSRPGIAAGASGLMRRCLDECVAYAKERKTFGTEIG
                     NHQMIQAMLADMAIRIEATQLLYRKAAWNLDHGVREPIVSSFAKAFGADSAVQTATDA
                     VQIFGGNGYVREYPVEKLYRDAKLLQIYEGTSQIQRIVIARQLLAG"
     gene            7173..>8830
                     /locus_tag="PK710_11830"
     CDS             7173..>8830
                     /locus_tag="PK710_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOT10456.1"
                     /translation="MTDQIEMTPEEGSQEESFASRFEQTTAASGFGKEGEIVKGRVIQ
                     IARDHVVVDIGGKSEGVIPLSEFSDGSEKALSPGELVDVFIEARENDDGLVSLSKEKA
                     DKMKVWDEISAACERDEIIDGTISQRVKGGLQVTIRGGVKAFLPGSQVDLRPIRNLDK
                     LIGQTHQFKVIKFNKKRGNIVLSRRVLLEKERDEMKAKTLETLSEGQTVIGTIKNITE
                     YGAFVDLGGIDGLLHITDMSWGRVNHPSEVFNVGDEVTVKVLKYNAETERVSLGLKQT
                     QEDPWNHAEEAYPPGKRVHGRVMSITDYGAFVELEPGVEGLIHVSEMSWTKKVKHPSK
                     MLEPNQDIECQVLEVDSKNKRISLGLKQLEPDPWSMFTERYHPGDKIGGRVRSLTDYG
                     VFVGIEEGVDGMVHKSDLSWTVKVNNPGDLFHKNDEVEAIILSINHDDKKVSLGVKQM
                     WDDPWPDMLTNFPPGKVLQAKVVSIVDYGIFVRMADGIEGLIPISEVVEDENNKIKLG
                     DEVEAEISNVDVVDRRITLTMKDIGQHPVQEIAAPKAAQASTLG"
BASE COUNT         2028 a         2721 c         2219 g         1862 t
ORIGIN      
        1 acctgttttc catgcccaat cggttagttg ccaccttttc gctgttcgga atcctggccc
       61 tattgccgaa ctgccaactt ttcgtgcagt cggacctcga caaggtgtac tgcgaacagg
      121 agggagtcat tggccctccc gcatgtccct cgggtgcctc ttgtcagcgt gggcagtgcg
      181 tgatgtgctc gaacgcggaa acctgcggtg atggaatcga taataattgc aatgggttac
      241 gcgatgaggg gtgtgaggat gcgggcagcg gcgatggtga tgcgccgtgg gatgctgacg
      301 atgctcccgc gacacaagat gttttcgaac cgccagaacc caatctcgac gggcgcgtga
      361 caaccggatt gcaagttctt tacgcgttca tggaagggca ggggacaacc gtcaaggact
      421 acgctccgaa cctcgatccc atcgatttga agatcgaatc tttcgaccac gtgaaatgga
      481 aacctggtgg cggcttgcat atcgctggtg ctacccgcat agcagcctcc aacacacaac
      541 gcttgatcga tgcctgccgc aagcaaaacg ccatcactgt cgaagcttgg atagaacccg
      601 agccgaatga accgcagagc tttgcgcctt tcgctagcct gtctttcagc tcttccctct
      661 gcaacttcac gctcgcacag caaaaccatc aattcgcatt tcgtctgcga acaacgaatt
      721 ccgacaacca gggaatgcca acgcttctat ccgaaaccaa tgccgtcacg gatacgttga
      781 ctcatctcgt cgtcgtccgg aatcaaagtg gcctggttcg gttcgtcgtc gacggcgtcg
      841 aaaagggaaa acagcagcga aacggttcgt tttccaattg gggatcgaac gccatgctca
      901 ccgtgggcaa cgccttttcc gaagatccaa cctggcttgg caccctgtat ttgatcgccg
      961 tatattgcca tccgctgatg gatagcgata tcgccaaaaa ctacgccgtg ggcaaaccaa
     1021 cggcgcgcca atgaaatctt gttcgtcagg atgaagcagg cgttgtcgac gagtctttgg
     1081 gttgcgttcc cgtccgttga ccgagaaaaa atgatccggc ggcaacgagc gcgatcaaaa
     1141 gcaatgccgc cacgatacgc agcggcatgc cgaagcgcga aggctgactc gcgggagaag
     1201 aaagcttacg aaccgatggc gcttcgaccg cgggcgatga caaagtcgat ttccgttctt
     1261 gcggctccga cgaattcgcc tctgccaaaa gggaaggtgt cttgggttcc gtgtccgtct
     1321 cgggcgcagc ctcacgctcg gatggtttct tttccgtggt gggagaagaa cgtgacaagc
     1381 gtccatcatc gagcttgatt tcgtcatcgg aaaacgatgc gcgccccgac ttgcccaacg
     1441 cttgagaaaa cgcttttact tgttcctgca cgctttggaa acgatccgcg ggcgaacgag
     1501 cacaggatgt ttcgaaccac gtgtccaccg cgacgggaag ccccggaacc cgatccgtca
     1561 atttcggcaa tggctcggag catatctgga acaacaattc cccaaacgtt ttcccatcaa
     1621 aaaccgaact acccgtaagc atcgtgaatg ccaacgcgcc catcgaatac acatccgcac
     1681 gatgatcgac agtcttgagc ccccgcactt gttccggact catgaacatc ggtgtgccca
     1741 cgatcgtccc cgtgcgcgtt cgagcatggg aggcgtatcg agcttgcgtc attttcgcca
     1801 cgccgaagtc caataccttg gccacgtaat cagactcttc aggcgagatg gtgagaaata
     1861 tgttttcggg tttgatgtcc cgatgaatga tgcccttgtc atgggcaacg gataaggcca
     1921 tgcctacatg gcgaaaaatt cgctccgcat catcgatgga taccgctccc tgccgctcga
     1981 tccgcgcgtg caacgactcc ccttcgagat actccatgac catataaggt gtgccgtcct
     2041 ccaattcacc gttgtcaatg acttgaacga catgccggga gtgaagagat gccaccgctt
     2101 tggcctcacc gtcaaagcgt cgaagcattt cctccgactc gatatggatg atggaaatga
     2161 acttgaccgc gacttttcgc tccagatgaa tgtggcgcgc gagccagacc gtacccatgc
     2221 cgccttggcc gatcatcgcc acgagttcgt acttgtcagc aacgacatag cctttgctga
     2281 tgagcggtga ttggggcaga tgactgctta cgtagttggg gacggcttgc tccgcttctt
     2341 caaccatcag aacacgacct catggttctc cgtgccatca cgagcaccat gccgaaaagt
     2401 ccgcggcgga ttatcccatc gcccgcgaca tcggtccact tcaatcctat ccatgacgaa
     2461 tccccacgcg cgcgattggc atgaaaaaaa acaggcttcc aaaattgtca tcggcctgct
     2521 gccgatcacg ctaccaggaa gaccgcccat cgaactataa cctgttgatt ttatatgtaa
     2581 tttgcttgcc attgacccgt gctatcgtcg aagcaatact gctcgcaacc ttcccatctt
     2641 ccacgcgatc gcaccgattc atgagcgaat ccattttcga tcatactgct ttcaatgctc
     2701 gggtatttca ccccagggag gacgaaggct ccgcgccccc aggtgccgaa gacctcataa
     2761 tccctgtcga acatgacatc cgcctgcatg ttcgtgtgta tcccgccccc tctgcccaaa
     2821 gagccgtcct cctttttcac ggcaatggcg aactcgtcgc ggattacgac ttcgtggctc
     2881 accattacct gcgcatgaac acgaccctgg ccgtcgtcga ttatcgtggc tacggacgta
     2941 gcgaaggcga tccccgtttt cgaacgatga tcgaggacgc acccaaagtc gtcgaagcgc
     3001 tccaacccgt gctctcacgc gaaacccgat tgccccttgt cgttctgggt cgctctcttg
     3061 gcagcgcctg tgccgcggaa atcgcaaaac gttctcccga cggtgtcgtg ggtttggtct
     3121 tcgacagcgc cttttccaac ctgaaagcat tcgcccttcg acgcggcagg caatacgatg
     3181 ttcctctttc ggagcaggac tacgaagcct tttgtcctct tcgaaaactc gcctcatgcc
     3241 acttgcccat cctcatcatg catggcgccc aagacaccct tttggctccc accgaagccc
     3301 agatgctttt cgacgccacg gcctcagcgc gcaaacgcat tgttttcatt gaagaatatg
     3361 gccataacga tctcttctac gcccccgctt attggcaaaa cctcacatct tttttggaag
     3421 aaatagcttc gtgattaccc ccaaagcgct cattttcgac ctcgatggct tgttagtcga
     3481 ctcggaaccg atatggttcg aagtcgaagg cggtttcctg gcggaattcg gccacatctg
     3541 gacgatcgaa gaagctcacg cctgtatggg gcagggtacc ccaaacaccc ttcgtatctg
     3601 gaaacaacgc tacggcatcc ccgtcgacat cccacgggat acggagcgga tcatcgaccg
     3661 aatgatcgcc cgtgcccatg aaatgcctct aaaaacaggg gcaattcagc ttctcgagca
     3721 cgcgcgtcaa cagcgttgcc ccatggctgt cgcttcgtcc tctccccaaa aactcatctc
     3781 ctccgtgctg gccgccaaac aaatcgattc gttttttcga gcgatcgtgt cggggcaaga
     3841 cgttccctgc tcgaagcccg cgcccgatgt cttccttcgc gccgccgaat gtctcgatat
     3901 cgaccctgcc tattgcgtgg ttctggaaga taccctggcc ggcacacgcg ccggaattgc
     3961 cgcgggaatg cgcgtggccg ccgtgccttc ctccaacccc cacgagatcg cttccctcgc
     4021 cacatgggtc cttcgcgacc tgaagcaagc catcgacgtt ctttttggat cgtgatggct
     4081 cgccatcctt tgtcctgcca tccagctaca caacgcaacc cacgcaacag gtggcaatca
     4141 cctcagcata ccaaacacga agaagaatcc tgcaaggaag cctagaaagg cgagcaagag
     4201 aaacacgatg acggtaaccc aaatccacat cgataagcgc gaatgattgc gatgatcaaa
     4261 acgtgggtcg ccccacggcg caggggagga cccaccactc gcaacaggtc cgcccgtcgc
     4321 cggaggactc gcgcccaacg atacagattg gtgaacataa agggatgacg catgcgcaat
     4381 cggctgcatc cccggtacca ccaccacagg cgcagagcca ccgccctgtt gcggcatggg
     4441 tggcattggt cccacgaccc cccgcatcac cgcgtgtacc tgcccagcca gtttcgccgc
     4501 ttcgagatga tggggatacg cggtaccgca aaaacgacac gccggaagca tataatcctc
     4561 aggcgtgaga cttgctccgc agttcaaaca ccgatcattt acttcgttca ctgcgcactt
     4621 accgcgtttt caggcaccca catctgctga ccattctgaa aggcgacgag aactttcccc
     4681 ccggatactt gcaccacctg ccctggatat cggttcccat tcgaccacgc aaccatcacg
     4741 gtcattcccg gcccaatgcc cttggcgatc gctcccatcc ccatggcccc gagcactcca
     4801 cgacccaaaa aacctacgtt cttcgacaca tcgccatagg ccgccgccgc ttgcgcgttg
     4861 gcttgcgcca ccgcttgcgc cacgtctgct tgcacctgaa gacccaccat ctgattctga
     4921 tactgctgcg ctgctgcttg aggcgttgct gcttgaggcg ttgctgccgg agtcgctgca
     4981 ttcgatgaac cactgcctcc agcctgccgg agaaacgtca cgggacgatg agcagcgccg
     5041 ctgcgaatac gctgcgcttc cgctgtcacc gacggatgaa acgactgata cgccatcata
     5101 ttctgaccga tacgcgagtt ccacgttccc gctatcatcg tccatgtcgt catgtcgatt
     5161 ccatacgcac gcaaggctcc ctcgatccca taagcctgaa ccgccgcgtt gagcgcgacc
     5221 cagtcgtcat agcttacgtc cacgctcccc ttcttcttcg acaacgcagc ctgatacaac
     5281 gacatataac gtgtagctac ctgacccatc aacgacatgt cttgcattcg cgccgtccag
     5341 ccagcaacgg cagcctgcca atccgcacgg gatacaccaa gactttccac aatgcgcatc
     5401 tgcgcctcgg tatcattcag atggtcagaa atctcggcgc tcaactcggc ataccgctcg
     5461 agcgaaatac cttggatggg ggaatacgga tcattcatga agtgaaacga tacgcggatc
     5521 caacggcgtg tccaggccag cagacagaaa tcgttgaaga gaacgcagaa aaaaagaacg
     5581 gtcgatcgac cttgccttcg cgctccccgc ccactgttcc cgaaatccaa tgaacgttcc
     5641 ccagcgccgt cctgggaaaa ggggatcgaa tcatggttat gttattgatt taaaacataa
     5701 atttgcatca cccggccaga agctgccggg cgatcacgat gcgctgtatc tgactggtgc
     5761 cttcataaat ctgcagcaac ttggcgtcgc gatacagctt ctccaccgga tactcgcgaa
     5821 catacccgtt gccgccaaag atttgcaccg catccgtggc cgtttgcacc gcgctatccg
     5881 cgccgaacgc tttggcaaac gaagatacga tcggctctcg cacgccatga tcgaggttcc
     5941 acgccgcttt gcgatacaac aattgcgtgg cctcgatgcg aatcgccatg tccgcaagca
     6001 tggcctgaat catctgatga ttgccgatct ccgtgccgaa ggtcttgcgc tctttcgcat
     6061 aagccacgca ttcatccagg caacggcgca tcaatccgct tgctcccgcg gcgattccgg
     6121 gacgcgattg attgaacgtt tccatcgcca gtttgaatcc tttgccttcg ggagcgagta
     6181 gattttgctt gggaacccga acgtcctcca aggttacggt agccgtatcc gatgctctct
     6241 ggcccaactt gtcttcatgt ttgcccggat gaaggcctgg cgtatcgcga tccacgatga
     6301 atgccgcgat gcccttatgc ttcagcgccg ggtcgatggt cgcgaaaatc acgaaaaaag
     6361 aagccaaatt ggcattcgtt atccaggcct tctccccgtt gagcacgtag tcatcgccga
     6421 ctttggtgaa tttcgttttc aaggccgcga catcgctgcc cgccccggct tccgtcgtgg
     6481 cataggcgca gaacttcggc tccgaagcca acatgccgag gtattttttc ttttgctctt
     6541 ccgaaccggc gagcaagatc ggcgtgagcc ccagcgtatt ggccatgatc gtggtctcga
     6601 ccccgatgca accccacgcc aattcctcgg cgatgaggca gttatccagc tcgtgaagcc
     6661 caggtccacc gtaagattcg gggatcgtgg gattgaccaa ccccagttcc caggcttcct
     6721 ggaagacgtc gacaggaaac tcgctttttt cgtcacattg cgccgcaatg ggaacgattt
     6781 tttcctttgc aaaacgatgc gccatgtcgg taagcgcctt ctgctcctcc gacaacgtga
     6841 aatcgagcat ctgtagcctc ctttgcgatg atgtcgccac attaccgcat cacgaatgcg
     6901 atgcgaaccc ccgcaacgtc atcccgtggg gcatctagcc cgcgatcatt cccgtatcag
     6961 ccgaattgga ggctcgccag ccccccaccg gggcgctacg ggcctccaag tgctttcccc
     7021 cacgacaggc cgcccgggcg aaaccgtcat ccagggactt tttttgcttt gacttgcccc
     7081 tttccgtgaa gtacgcacct cgcgggttcg gtcgcccagg gtgtacccgc gcgtccgaat
     7141 actactagga aagctgaggg taaacataca acatgacaga tcagatcgag atgactcccg
     7201 aagaagggag ccaagaggaa agcttcgctt cacgtttcga acagacaacg gcggccagtg
     7261 gttttggtaa agaaggtgaa atcgtcaaag gacgcgtgat ccaaattgct cgtgatcacg
     7321 tggtcgtcga catcggtggt aagtccgaag gcgtgatccc cctatcggaa ttctccgacg
     7381 gatcagaaaa ggcgttgtcg cccggtgaac tcgtggatgt tttcatcgaa gcccgggaaa
     7441 acgacgacgg cctcgtctcc ttgtccaagg agaaggccga caagatgaag gtctgggacg
     7501 aaatctccgc tgcttgcgaa cgcgacgaaa tcatcgatgg caccatcagc cagcgcgtca
     7561 aaggcggact ccaggtcacc atccgcggcg gcgtcaaagc tttccttcct ggttcgcaag
     7621 tcgacttgcg acccatccgc aatctcgaca aattgatcgg ccaaacccat caattcaagg
     7681 tcatcaagtt caataaaaaa cgcgggaaca tcgtgttgtc tcgacgcgtg ctgctcgaaa
     7741 aagaacgcga cgagatgaaa gccaaaaccc tcgaaaccct ctccgaagga cagaccgtca
     7801 tcggaaccat caaaaatatc accgaatacg gcgctttcgt cgacctcggc ggtatcgatg
     7861 gcttgttgca tatcaccgat atgtcctggg gacgcgtcaa ccatccgtcg gaagtattca
     7921 acgtcggcga cgaggttacc gtcaaggtcc tcaagtacaa cgccgaaacc gaaagggtca
     7981 gcctcggtct caaacaaacc caggaagacc cgtggaatca cgccgaagaa gcgtatcccc
     8041 ccggcaaacg cgtccatggc cgcgtgatgt ccatcaccga ttatggcgcc ttcgtcgaac
     8101 tcgagcccgg tgtcgaaggc ttgatccacg tttccgaaat gagctggacc aagaaagtca
     8161 aacaccccag caaaatgctc gaacccaatc aggatatcga atgccaggtg ctcgaagtcg
     8221 attcgaaaaa caagcgaatc agccttggcc tcaaacaact cgaacccgac ccgtggagca
     8281 tgttcaccga acgatatcac cccggtgata agatcggcgg acgagtccgt agcctcaccg
     8341 actacggtgt cttcgtcggt atcgaagagg gcgtcgatgg catggtccac aaaagcgact
     8401 tgtcctggac cgtcaaagtc aataaccccg gcgatctctt ccacaaaaac gatgaggtcg
     8461 aggccatcat cctgtcgatc aaccacgacg acaagaaagt atccctcggc gtcaagcaga
     8521 tgtgggatga cccatggccc gatatgctca ccaacttccc tcccgggaaa gttctgcagg
     8581 ccaaggtcgt cagcatcgtc gattacggca ttttcgtgcg catggccgat gggatcgaag
     8641 gcctcattcc catctctgaa gtcgtcgaag acgaaaacaa caagatcaaa ctcggcgacg
     8701 aagtagaggc tgaaatctcc aatgtcgatg tcgtcgatcg ccgtattacc ctgaccatga
     8761 aggacatcgg tcagcacccg gttcaggaaa tcgccgctcc caaggccgcg caagcctcca
     8821 ccctcgggga
//
LOCUS       DAOSOR010000193         8802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_7936_length_8802_cov_3.756717, whole genome shotgun sequence.
ACCESSION   DAOSOR010000193 DAOSOR010000000
VERSION     DAOSOR010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8802
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7936_length_8802_cov_3.756717"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PK710_11835"
     CDS             complement(<1..287)
                     /locus_tag="PK710_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10457.1"
                     /translation="MTLSRPTMTPPRHPRAVAMLIAWLILAIPSAAYASDVADRFTSA
                     LEKGPIYAALAAALGGFLTSLTPCVYPMIAVTVSVFGARQSKSRREAMLLST"
     gene            289..411
                     /locus_tag="PK710_11840"
     CDS             289..411
                     /locus_tag="PK710_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10458.1"
                     /translation="MNKPYGTGGCVCVGGSSALVSEPSAGAIDSGGKIIEGIFV"
     gene            complement(389..682)
                     /locus_tag="PK710_11845"
     CDS             complement(389..682)
                     /locus_tag="PK710_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10459.1"
                     /translation="MSPSRFAPLVLISLLAFGCRYATEKDCEHIIDRIVELELKEQGI
                     TNPDLIEQRKAETRRKKRDELITGCVGKRITDAAMTCIDNASSSKDITQRCLR"
     gene            769..1506
                     /locus_tag="PK710_11850"
     CDS             769..1506
                     /locus_tag="PK710_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT10460.1"
                     /translation="MRERAKPEDWTPETMLQRAIAAKTATTRAKYARLGLSTEQPLDR
                     TTQSLLLRQLYLSYFESENFEKALEIAEQILALGIMLDVCRQDMARTLLSLGQLDAAV
                     EHLRLAARAAPASRRSFHWWTLGSVLYLAGRLEEAEGALRRAVRWGTTARPLYDAQLA
                     LIQLEMGQSVADVDDVVERLEEAPCGQGYGRFVLGMLCLRAGFASKGEHYLRLFLKRL
                     HQGRKATALSLSGEIAIAQEQLRRLAG"
     gene            complement(1510..1842)
                     /locus_tag="PK710_11855"
     CDS             complement(1510..1842)
                     /locus_tag="PK710_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10461.1"
                     /translation="MTCLRLSALALCVGCAALPPPTLPPHPAGCPVTVYRVGVPKNVE
                     FTTLGDVQAPCGKNDADSDCIRALQDEVCKLGGNIVVEVPEAPEPQSDSMLRYRGVAG
                     TTEDPSAR"
     gene            complement(1839..2738)
                     /locus_tag="PK710_11860"
     CDS             complement(1839..2738)
                     /locus_tag="PK710_11860"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOT10462.1"
                     /translation="MHFQDLILTLQAFWANHGCVIVQPYNSEVGAGTYNPATFLRALG
                     PEPWNVAFVEPSRRPSDGRYGENPNRLQQFHQLQVILKPSPLDLQDLYLESLRAIGTE
                     PLAHDVRFIEDDWESPTLGAWGLGWQVWIDGLEVSQYTYFQQVGGIDCKPVTGELTYG
                     LERIAMYLQDVDNVYDLQFNDRVRYGELFQRGEWEWSSYNFEFADVAAHFAAFDRCEA
                     EAKRLLSLSKDPLRTLVLPAYDYVVKAAHHFNVLDARGAIGVTERQRFIGRVRLLARQ
                     VAESFVAQRQALGFPLLHLPESP"
     gene            2931..3080
                     /gene="rpmH"
                     /locus_tag="PK710_11865"
     CDS             2931..3080
                     /gene="rpmH"
                     /locus_tag="PK710_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOT10463.1"
                     /translation="MKRTYQPHNRSRLRTHGFRKRNSSASGRKVLRNRRLKGRKRLAP
                     TTYKK"
     gene            3098..3484
                     /gene="rnpA"
                     /locus_tag="PK710_11870"
     CDS             3098..3484
                     /gene="rnpA"
                     /locus_tag="PK710_11870"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOT10464.1"
                     /translation="MIAEDHRSLRLTRDRRLRHRRDFVRIQSTGVRVTTEHFVFLMAP
                     RTAPAPSRLGITVTKKVGCAVVRNRAKRLIRETFRTLSERLPRDVDVVVIVRKALHGQ
                     TTNSVMREWLAVEALLHRRAAMGRST"
     gene            3481..3723
                     /gene="yidD"
                     /locus_tag="PK710_11875"
     CDS             3481..3723
                     /gene="yidD"
                     /locus_tag="PK710_11875"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOT10465.1"
                     /translation="MIVKLLLLMIRAYQLTISPLLGPVCRFEPSCSRYTMACLRDHGL
                     FRGSWFSIKRICRCHPFHPGGYDPPPLPTSGHRPHS"
     gene            3742..5550
                     /gene="yidC"
                     /locus_tag="PK710_11880"
     CDS             3742..5550
                     /gene="yidC"
                     /locus_tag="PK710_11880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOT10466.1"
                     /translation="MERSTILRWIIIALAIWLAYTYLPKLWGGGNGSSSFQPLGPESA
                     CSTHSPCVPEVCDAEGKNCKPAERKPETYCELQGDRFSAQFTTQGASLKSLRLQGERY
                     RNPSDSPKGAGEPIDLVTSPDVEQRRPLRFDWRDQGATGKDDAQVLYDAFDWELKENS
                     PSQCVFTYTDNMVELRKVVKPGSRPFEIDIEASVKNLSENKRKHALSVEVDTWRYQSE
                     VESHFGRPSPFMTHVSCAHDNKNTSKAITDFEPKDFNDPEFESGWFINRGQIQHAAVS
                     DSYFAQALIPLEPAANRCLLQIEERWNAGQFTEKSKDPNYGAAYRARLSYPPQELSKG
                     EASTYRITAFLGPKERDVLSSAASGKYGLDQLVDLGFFSVIARVLLRYLVWLHAVIGN
                     WGIAIILMTISIRILLFPLTWKSIQAGLAMRKLRPELDAINKKYANDPQGKNIATMEL
                     WKTHKVNPLGGCLPVMFQMPVWWALFTSLQTAVELYHTPFLWFQDLSARDPYFIIPFI
                     LGGTMILQQKMMPMQMDPMQQKMMTYVMPAVFTAMMLFLPSGLAVYMLTNAIIGILQQ
                     LGIEKYYASTGVAAATAGSGITVREKPDKSLPDTKK"
     gene            5555..6202
                     /locus_tag="PK710_11885"
     CDS             5555..6202
                     /locus_tag="PK710_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOT10467.1"
                     /translation="MEPFDHDASRPSHDPFEAFDDEDNAEPYETTFDQGQAEEGEAME
                     NENDPTASDFDRNQPPDERAQSAKDFLRQVLIEMDMDVSIERHPPRPDSSIDEIHLEI
                     VGHDVGRVIGKKGQVLSALQYLVNRAVNRPGLPRRHIVLDAEGYRHRRETNLASLARR
                     LGEQAAKEGKIITFEPMSPRDRRVVHLALAKFPHVVTKSDGEGEERRVRIIPVRR"
     gene            complement(6359..7252)
                     /locus_tag="PK710_11890"
     CDS             complement(6359..7252)
                     /locus_tag="PK710_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10468.1"
                     /translation="MNRTLRPSQWCLWLISPLMLVGSAWAQSDEPLPPLPADPDAPPV
                     DLPPATPVAPPAAQPSPPASPLYPPAPGSGYGTPAQYGYPSPPPAPPVPPAQPPTGVY
                     LHDGFYLRMALGLGLHNITVAPHPGDTIHASTQNLLFDVGVGGTPMDGFAIGGRILEM
                     FAANPSFGSDSAEKDLSRNTSLSVLQFFTDVYPTPDRGLHFMAAVGPATYSFSRDTKD
                     SEEFSVMGAAFSIGAGWEAWVGQQWSMGGMLALHWMVARDSNIRLLAPSSSRNASNTE
                     SDEDVSLDALMPSLTWTITYH"
     gene            complement(7249..8691)
                     /locus_tag="PK710_11895"
     CDS             complement(7249..8691)
                     /locus_tag="PK710_11895"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOT10469.1"
                     /translation="MIDRLERLLGRENVVSDPSALAAYDDDYSEVESVLPAAVVLPTT
                     VPQVQAIVRFAGETGICLTPRVAGTNVGGLAIPTPRSIVVDFRRMNRVLEVYPDDRVA
                     VIEPGVTQQQLKDHLDSLGLPLTFGFSLGPRRSSVLANCCLDGLHNRSLKYGAMGQSV
                     SGFEVVLADGSLMRTGAWALSDIPFARSPLPDLSGLFVGWQGTTGLVTKVGFHLAHKH
                     PFSERLFVLAYSVRGTFEAMRRLSRMEFCDDIGGLSWPTGKMMLGVTKPHPIADDGEP
                     RFFLYVDLTAERPEQLVLKRQMLEDVLSELRQEGQRYEEPIGIETLVTLNPAMGAFAS
                     FPTDLGFLTDHPGGGLTWVGTYGPLSRFDDAAHEGIEVMVRHGFAPAIVSRSMKEGHF
                     GVLRFLSLFDKKKPQEIEIVKRVNLELLHAMTARGFIMYKTPSWALREWLSHIDPGFL
                     SMMRKVKTLLDPQGIFNPGKLLLPWPENEG"
BASE COUNT         1987 a         2410 c         2617 g         1788 t
ORIGIN      
        1 gtggacagga gcatggcctc gcgccgggac ttggattgtc gcgcgccgaa tacgctgacg
       61 gtcacggcga tcatcgggta cacgcaaggg gtgagactgg tgagaaagcc tcccagggca
      121 gcggctaacg ccgcgtaaat aggtcctttt tcgagagccg aggtaaatcg atccgccacg
      181 tcggaagcgt aggccgcgga aggtatggcc aagatgagcc atgcgatgag catggcgacg
      241 gcacgggggt ggcgtggggg ggtcatcgtg ggacgagaca aagtcatcgt gaacaaaccc
      301 tatggtacgg gaggatgtgt ttgcgtaggg ggctcatcgg ccctcgtgtc agagccaagc
      361 gcgggcgcca tcgattcagg gggaaaaatc atcgaaggca tctttgtgtg atgtctttcg
      421 aggaggaggc gttgtcgata caggtcatgg cggcgtcggt aattcgcttg ccgacgcagc
      481 cggtgatgag ttcatcgcgt ttttttctac gagtttccgc ttttcgttgt tcgatgaggt
      541 ctgggttggt gatgccttgt tctttgagtt cgagttcgac gattcgatcg atgatatgct
      601 cacaatcctt ttcggtggcg tagcggcaac cgaaggcgag cagggagatc aacacgagcg
      661 gagcaaaccg ggagggggac acgcgcggca gtgtactcga ccctcgtccg cgcgcaacca
      721 tggtggattt ctggacgaat tatgcaggct tgctacgcta aaaccatagt gcgcgagcga
      781 gcgaaacctg aagactggac gccggaaacc atgttgcagc gagccatcgc ggcaaaaacg
      841 gccacgacaa gagccaagta tgcacgtctt ggattgtcga cggagcagcc tttggatcgg
      901 acgacgcaat cgttgttgct tcgtcagcta tatctttcgt atttcgagag tgagaatttc
      961 gaaaaagcgt tggagattgc ggagcagata ctggcattgg gcatcatgct ggacgtctgt
     1021 cgtcaggaca tggcgcggac gttgttgtcg ttggggcaat tggatgcggc ggtagagcat
     1081 ttgcgattgg cggcgcgcgc agctccagcg agccgtcgtt cctttcattg gtggacgttg
     1141 ggcagtgtgc tttacttggc ggggcggctg gaagaagcgg aaggggcgtt gcgacgagcc
     1201 gtgcgttggg ggacgacagc tcggccgtta tacgatgccc aactggcatt gattcagctt
     1261 gaaatggggc aatccgtggc cgatgtggac gatgtggtgg aaaggctgga ggaggctcct
     1321 tgtggtcagg gttatggtcg gttcgtgctc ggcatgcttt gtcttcgcgc aggttttgct
     1381 tcgaaaggcg agcattactt gcgtctgttt ctcaaacgac ttcaccaggg gcgcaaagcc
     1441 acggctcttt cgttgtccgg agagattgcc atcgcccagg agcaacttcg acggctggct
     1501 ggttgaggct catcgagcgg aggggtcttc ggtggtgccg gcgacaccgc ggtatcggag
     1561 catggaatcg gattgtggtt cgggggcctc ggggacttcg acgacgatgt tgccgccgag
     1621 tttgcatact tcgtcttgca aagcgcgaat gcaatcgctg tcggcatcgt tttttccgca
     1681 gggagcctgg acatcaccga gggtggtgaa ttcgacgttt ttggggactc cgacgcggta
     1741 gacggtgacg gggcagccgg cagggtgtgg tggtaaggtt ggagggggaa gagcagcaca
     1801 ccccacacaa agcgccaagg cagacaaacg taggcaggtc atggggactc cggaaggtgc
     1861 agcaggggaa agcccagggc ttggcgttgt gcgacaaagg attcggcgac ttgacgtgcg
     1921 agcaagcgga cgcgtccgat gaaccgttgt cgttcggtga cgccaatggc gccgcgggca
     1981 tcgaggacgt tgaagtggtg tgcggctttc accacgtaat cataagctgg caagaccagg
     2041 gttcgaaggg ggtctttgga tagggaaagc agacgtttgg cttcggcttc gcagcggtcg
     2101 aaagcggcaa aatgcgcggc tacgtcggcg aattcgaagt tgtaggaaga ccattcccat
     2161 tcgccgcgtt ggaatagttc gccgtagcgg acgcggtcat tgaattgcag gtcgtacacg
     2221 ttatcgacat cttgcaggta catggcgata cgttcgaggc cgtaggtgag ttcgccggtc
     2281 acgggtttgc agtcgatgcc accgacttgt tggaagtagg tgtattgtga gacttcgagc
     2341 ccatcgatcc acacttgcca acccaaaccc caggcaccga gggtagggga ttcccaatcg
     2401 tcttcgatga agcggacatc gtgggcaagc ggttcggtgc cgatggcccg caaggattcg
     2461 aggtacaagt cttgaaggtc gaggggggag ggtttgagga tgacctgcag ttggtgaaac
     2521 tgctgtaaac gattgggatt ttcgccgtag cggccatcgc tgggtctgcg ggacggttcg
     2581 acgaaagcga cgttccacgg ttctgggccc agggcgcgca ggaaggtagc ggggttgtag
     2641 gtacccgctc ccacttcgga attataaggc tgaacgatga cgcagccatg attggcccag
     2701 aaggcttgca gcgtgagaat caggtcttgg aagtgcatcg gtggttccgt tgtcgagggt
     2761 cgatttaccc tgcctcgacc ccagcgtaaa ccagaagtga aaggatgaaa tagcaacttg
     2821 cctgacgtgg ggtcgagcgg ggccccatcg gttcgaactt cgggcgttca taggtcttga
     2881 ctacatgccg tcaagcgtgc cagagtcccg gcccctttga ggtagcagcg atgaaacgca
     2941 cgtatcaacc ccataatcga agccgtcttc gcacccacgg ctttcgtaaa cgcaattcct
     3001 cggcctccgg acgcaaagtt ctgcgcaatc gtcgtttgaa gggacgcaag cgcctcgccc
     3061 ccacgaccta caagaagtag tgtctgcctc ttgcgcggtg atcgctgaag accatcgttc
     3121 tttgcgtttg acgcgcgacc gacggcttcg tcaccgtcgg gacttcgtcc gcatccaatc
     3181 gactggagtt cgcgtaacga cggagcattt cgtgttcttg atggcccctc gcacagcccc
     3241 tgctccgagc cgacttggca ttacggtcac gaaaaaagtc ggttgtgcgg tggtgcgcaa
     3301 tcgggccaaa cgactcatcc gcgaaacgtt ccgaacccta tccgagcgat tgccccgcga
     3361 cgtggacgtg gtagtcatcg ttcgcaaagc cttacacggt caaaccacaa atagcgtcat
     3421 gcgcgaatgg ttggcggtcg aagctttgct acatcgtcgt gcagccatgg ggagaagcac
     3481 atgatcgtca agctgctttt gctaatgatt cgcgcgtatc agcttacgat ttcgccgttg
     3541 cttggcccgg tctgccggtt cgagccttct tgctcgcgat atacgatggc ttgccttcgt
     3601 gaccacggtt tatttcgcgg ctcatggttt tccatcaagc gcatctgcag gtgccatcct
     3661 ttccaccctg gaggctacga tcctcctccc ttgccgacct ccggccaccg tcctcattca
     3721 tgaggccgag ttgaaagcaa catggaacga agcacaatcc tccgctggat catcatagct
     3781 ctcgcgatct ggctcgctta tacgtacctg cccaaattat ggggcggggg aaatggctcg
     3841 tcgtcgtttc aacctcttgg accggaaagc gcatgcagca cgcattctcc ttgtgtcccc
     3901 gaggtatgtg atgccgaagg caagaactgc aaacccgcgg agcgcaaacc cgaaacctat
     3961 tgtgaactgc aaggggaccg gttttcggca cagttcacga cgcagggagc ctctctcaag
     4021 tccttgcgtt tgcaagggga gcgctatcga aatcccagcg attcccccaa aggcgccggt
     4081 gaacccatcg atctggtcac ctcaccggat gtcgaacaac gaagaccgct gcgattcgat
     4141 tggcgagatc aaggtgccac gggcaaagac gatgcgcagg tattgtatga cgctttcgat
     4201 tgggagctga aggaaaacag cccttcgcaa tgtgtgttca cctacacgga taacatggtc
     4261 gaattgcgaa aggtcgtgaa gcccgggtcg cgtccgtttg aaatcgacat cgaggcgagc
     4321 gtcaagaatc tatcggaaaa caagcgaaaa cacgcgctat cggtcgaagt ggacacgtgg
     4381 cgttatcaat ccgaagtgga atctcatttc ggacgtccat cgccattcat gacccatgta
     4441 tcttgtgccc acgacaataa gaatacgagc aaggcgatca ccgatttcga acccaaggac
     4501 ttcaatgacc cggaattcga gtccgggtgg ttcatcaatc gaggacagat ccagcacgcc
     4561 gccgtatcgg attcttattt cgcgcaagcg ttgattccgt tggagcccgc cgccaatcgt
     4621 tgcctgctgc agatcgaaga acgttggaac gctggacaat tcaccgaaaa atcgaaagac
     4681 ccaaactatg gtgcggccta tcgcgcacgc ttgagctatc cgccccaaga gctttccaaa
     4741 ggcgaagcct ccacgtaccg cattacggct tttctcggtc ccaaagagcg cgacgttctc
     4801 agcagcgccg cttccggcaa atacggtctc gatcaactcg tcgacctagg atttttcagc
     4861 gtcatcgccc gagtgctgct ccggtatctc gtctggctgc acgcggtcat cggcaattgg
     4921 ggaattgcca tcatcctgat gaccatcagc attcgaatcc ttctatttcc cctgacatgg
     4981 aagtcgatcc aagcgggact cgcgatgcga aaactccgtc ccgaactcga tgccatcaac
     5041 aaaaaatacg ccaacgatcc ccagggcaaa aacatcgcaa ccatggagct ttggaaaacc
     5101 cacaaggtca atccgctcgg tggatgcttg cccgtgatgt tccaaatgcc cgtgtggtgg
     5161 gcgctattca cctccctgca aacggcggtc gagctttatc acacgccatt tctttggttt
     5221 caggatctat ccgcgcgaga cccatacttc atcattccct tcatcctcgg cggcaccatg
     5281 attttgcagc aaaaaatgat gcccatgcag atggacccca tgcagcaaaa aatgatgacg
     5341 tacgtcatgc ccgcggtctt caccgccatg atgctctttt tgccctcggg tctcgcggtc
     5401 tacatgctga ccaacgcaat catcggaatc ttgcagcaac tcggcatcga aaagtattac
     5461 gcttctaccg gtgtcgccgc cgctacggcc ggctctggca tcaccgtacg cgaaaaaccc
     5521 gacaaatccc tgccggatac caagaaataa ttccatggaa cccttcgacc acgatgcctc
     5581 tcgcccctcc cacgacccgt tcgaagcttt cgacgatgag gacaacgccg agccctatga
     5641 gacgacgttc gaccaagggc aagcggagga aggagaggcg atggaaaacg aaaacgatcc
     5701 caccgcatcc gacttcgacc gcaatcagcc ccccgatgaa cgcgcacaat ccgccaagga
     5761 cttcctgcgc caagtcctca tcgaaatgga tatggacgtt tccatcgaac gacatccacc
     5821 acgaccggat agctccatcg acgagatcca tctcgagatc gtgggccatg atgtgggaag
     5881 ggtgattggc aaaaaaggac aggtattgtc cgctttgcaa tatctggtca accgagccgt
     5941 caatcgaccc ggactgccca gacgacatat cgtgctcgat gcggaaggct atcggcatcg
     6001 acgcgaaacc aacctggcct cgttggcccg acgactcggc gaacaagcag caaaagaggg
     6061 gaaaatcatc accttcgaac ccatgagccc gcgcgatcgc agagtcgttc acctcgcgct
     6121 cgccaagttc ccccacgtcg tcaccaaaag cgatggggaa ggcgaagaac gtcgcgtgcg
     6181 catcatcccg gtgcgacgat agtagggatt gtcggagggt tgtcgcgacg cgcgcccgag
     6241 ccaaggggtg accgggagcg tgaagttgct tttcgcatca acgccacagc gtcctttccg
     6301 agctttcgga cagcgcgcaa ctatttgaaa ttacatcaat ttcccctatt cgctcccatc
     6361 aatgataggt gatggtccag gtcaaactgg gcatcagcgc atcgagcgat acatcctcat
     6421 cagactcggt attcgaagcg ttgcgggagg atgagggagc caacaaccgg atgttcgagt
     6481 cgcgggcgac catccaatgc agcgccaaca tgccacccat gctccattgc tgtccgaccc
     6541 acgcttccca accggcaccg atggagaagg ccgcccccat gacggagaac tcttcggaat
     6601 ccttggtatc tcggctgaag gaatacgtgg cgggaccgac tgctgccatg aagtgaaggc
     6661 ctcgatcggg cgttggatac acatcggtga agaattgaag aaccgaaagc gatgtatttc
     6721 ttgacaggtc tttctccgca ctgtcggagc caaagcttgg attggccgcg aacatctcca
     6781 agattctccc cccaatcgcg aagccgtcca tgggagtgcc tcctactccg acatcgaata
     6841 ggaggttttg tgtagaggca tggatggtgt cgcctggatg gggggcgacg gtaatgttgt
     6901 gcaaccccaa gcccaaagcc attcgcaagt aaaacccatc atggagatac acgccggtgg
     6961 gaggttgggc aggaggtacg ggcggcgcgg ggggtggact tggataaccg tattgggcgg
     7021 gcgttccata accggagcca ggggcagggg gatatagggg cgaagcgggt ggtgacggtt
     7081 gagccgcggg cggagccacg ggtgtagcag ggggaaggtc gacgggcgga gcatccggat
     7141 cggcaggaag cggaggcaac ggttcgtctg attgtgccca agccgagccc acgagcataa
     7201 ggggtgagat cagccacaaa caccattgtg atggtcgcag ggtacgattc atccttcgtt
     7261 ttccggccac ggcagcaaca gcttaccggg gttgaagatc ccttgcgggt cgagcagggt
     7321 tttcactttc cgcatcatcg acaaaaaccc cgggtcgatg tgggataacc attcccgcaa
     7381 cgcccaggag ggggttttat acatgatgaa tccacgagcc gtcatggcgt gcagcagttc
     7441 gagattgaca cgcttgacga tctctatttc ctgcggtttt ttcttgtcga aaaggctcaa
     7501 aaatcgcagc acgccgaagt gcccttcttt catggaacgc gacacgatcg cgggagcaaa
     7561 tccatgacgc accatcactt cgatcccctc gtgggcagcg tcgtcgaaac gtgacaacgg
     7621 accgtaggtc cccacccacg tcaatccccc cccaggatgg tcagtcaaaa aaccaaggtc
     7681 cgtgggaaac gaggcaaagg cccccatcgc aggattcaac gtcacgagcg tctcgatgcc
     7741 aatcggctcc tcgtatcgtt gtccctcctg acgcagctcg gaaagcacgt cttcgagcat
     7801 ttgacgtttc aatacgagtt gctcgggacg ctccgcggtt aggtccacgt aaagaaaaaa
     7861 acgaggctcg ccgtcatcgg cgatgggatg aggtttggtc acgccaagca tcattttgcc
     7921 ggtaggccag gacaagcctc cgatatcgtc gcaaaactcc attcggctga gtcgacgcat
     7981 ggcctcgaag gtgccccgca ccgaataggc aagcacaaac aaacgttcgg aaaacggatg
     8041 cttgtgggca agatgaaatc caaccttggt aacaaggccg gtggttcctt gccatcccac
     8101 gaacaatccc gatagatcgg gcagcggact gcgcgcaaaa gggatgtccg acaaggccca
     8161 cgcccctgtc cgcatcaaag aaccatcggc aagcacaacc tcgaaccccg atacggactg
     8221 gcccatcgcc ccgtacttga gcgaacggtt gtgcaaccca tccagacaac aattcgccag
     8281 aacagaagag cgtctcggac ccaaagaaaa accgaacgta agaggcagcc ccaacgaatc
     8341 gaggtggtcc ttgagctgtt gttgcgtaac ccctggctcg atgacggcga cccgatcgtc
     8401 gggataaacc tcaagcacac ggttcatgcg acggaagtcc accacgatgg aacggggtgt
     8461 ggggatggcg agccctccca cattggttcc cgccacccga ggcgtcagac aaatgcctgt
     8521 ttcccccgca aatcgaacga tggcttgtac ctgcggtacc gtcgtcggca ataccaccgc
     8581 cgcgggcaac acagattcca cttcgctata gtcatcgtcg taggcggcaa gggccgaagg
     8641 gtcggagacg acattttcgc gtccgaggag gcgttcgaga cgatcgatca tgcgccagct
     8701 cctggcggtg atacggaagg ggaagcggga atcgcgggta ccggggcggg aaccttgacg
     8761 tttttgatca tgaggggaag caaacgaagc aaggcgaagg ga
//
LOCUS       DAOSOR010000194         8785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_7958_length_8785_cov_4.403322, whole genome shotgun sequence.
ACCESSION   DAOSOR010000194 DAOSOR010000000
VERSION     DAOSOR010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8785
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7958_length_8785_cov_4.403322"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="PK710_11900"
     CDS             <1..1031
                     /locus_tag="PK710_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10470.1"
                     /translation="MGRFFVHQGQSSLDNLDALAPWILRLRRQARTRAPTRLIAAEKL
                     LVDAIFDVSQHPEEPLRWQQILLSLANIESVFVSGTGFAAGPVPPLNPKWVQAADDGS
                     PEFRLAVAFALQRIRQGKPDGVRRHWLPLNRQQRFETTGDRGSALARRPDVVMFGRDG
                     IGDALAVVERRLVEASQSGQRQWMLEAARRTDASLSDLTALLSGEVDITHTMNLAKAL
                     MAIDPYEWQRQPAPPSPPSFEQRWPDDSWVAIRLALLPWPLDHVQIPTDPAIVRRLSA
                     GDTASAVRLALQRLRAAGLRCPFSIPVGNPDGRLWGAALAFPISLRSARQLARRFDPA
                     STITEMMP"
     gene            1028..2035
                     /gene="cas7u"
                     /locus_tag="PK710_11905"
     CDS             1028..2035
                     /gene="cas7u"
                     /locus_tag="PK710_11905"
                     /inference="COORDINATES: protein motif:HMM:NF021156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated RAMP protein
                     Csb1/Cas7u"
                     /protein_id="HOT10471.1"
                     /translation="MSVDFQTLSTLPRLLFSIPLAPLQGSRFQPTGFPSLGAATFQTS
                     EGTSLLVESAQSMANRLEMTIWDEASQDVIETVKGISHVKVMREGKFLTDTILEAHRL
                     NSPYLLVESSKAFFNLLKEELGGLETGPINRRILAETLLKFDIGSLLHGVFLAKKELA
                     GGRLRIARAISAFIEADGAKVAASGGVKNDHVNPSGDTKAGFGNVPFARDEFTADTIT
                     LYVNIDLAQIRGYGLGKDVEELLIALALFKLRRLLSSNLRLRTACDLKATPTTIEASE
                     PKGFVLPTESDLATLVKEKIQATKDKMKVSEAKYDEPMKKGKEEPEDKPSDDRSENHE
                     D"
     gene            2038..3630
                     /gene="csb2"
                     /locus_tag="PK710_11910"
     CDS             2038..3630
                     /gene="csb2"
                     /locus_tag="PK710_11910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csb2"
                     /protein_id="HOT10472.1"
                     /translation="MVTIVFRFPGGRYHATPTGHHVNEGWIEWPPSPWRVLRALIATG
                     FTKLHWVTIPDPARSLIEMLADTLPEYVLPRAGTGHTRHYMPLASIDKGREKTTLVFD
                     SWADVGKGQLAIRWNVILPEEQTQLLRNLVESMSYLGRAESWVAGSLADDSWSPPPDS
                     LRAYPCEGSNRPGAGWEQVALLAPEPANEYSRWRESQMACMPQVTEKPKKKGKKKSTK
                     HESDIPEDLIACLLVDTTWLEASGWNQAPGSRKALYWRPFRALEPGAPLRRRAMHNAP
                     PVEAMLLALSTPSGNMHALPPITRVLPQAELLHRSLVSHIARLSPSHHGTLTGKSENG
                     SPRRGHSHVHLLALDLDSDGHLDHFLLWAPAGLDAACQEAVRATRKTYMKGGVGELQL
                     AISGAGSLGDLRTAPGPEGKNLLQSIGVSPVWTSATPLVLPRHLKSKGRHTLEGQIQE
                     ELASRGLPPATSIEILSQQQDFLRRFPHFVRVRRSGPPPPADQGYALSLSFDQPIAGP
                     LCLGYGSHFGLGRFVTSSPPPS"
     gene            complement(3906..5165)
                     /locus_tag="PK710_11915"
     CDS             complement(3906..5165)
                     /locus_tag="PK710_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOT10473.1"
                     /translation="MAPKIVFDSTQRFDCRDCNARCCRLPVAIPLNTQEIERFTKEPW
                     VRERVGDAGMAWIAKGVVPIRDVDQMPQCVFLDDDELCSMQKKLGHDFLPQTCQSFPF
                     GFVSNEKDQLVAQMSQLCPSIRDGIGDLVTVQRLRDKLSQKGGSERMSKAMATRHGRI
                     LTQSQYMRVVHKWLERLERQAPPLELLSELYDWTKAFEDALPGSSEKPDDAGVTEALA
                     RADETQLFRFQLSTSLPFQARTLHAYVLGGLSYPSRVALHHRIDPRSAMQSLRVLWTK
                     LKWILCRGSVHVLYIRNPVPLGSVDGVERFLGTEKARPINRLLRLVLERRQIFRDPRF
                     LMEVIIDLALSTIVISRYARCRAASEGRTEVQAADIHEGISIVELALLGHASQRQAGA
                     TLRNMRRTLIAARENYLRLLASENSTS"
     gene            complement(5232..6305)
                     /locus_tag="PK710_11920"
     CDS             complement(5232..6305)
                     /locus_tag="PK710_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10474.1"
                     /translation="MRMVATIVTVALSICGCSGPDTSALVANDASPGVVVDASVSNDA
                     SGADGNMNDREIEASMDDVAPADSSFVHDGPSVFTFFHGIPNARALRLCFLAGKATSM
                     VPLPVLAMPSDTLGLLYGSAFSAQSLEGADLAAQDIQPVVYTGAVETIQGRRCDELEA
                     VPEKITRTILPVVPAGTLSRGRSVLMIAAGCAPMQVEDPDTQSVCGDDYSSEKGNVTM
                     MVASMDRSSPAGAMGVQVISASKATEKLGLDHVTAFPYKATVLAASLSPGKIAPRPPS
                     KELSVANLGTSSQENLLRVFVDNSMTPVAESLLTDALDRGGLSLSSLQDGKDYTVVVA
                     GPNPGRQGDWFHSMVITVLPSDP"
     gene            6503..7675
                     /locus_tag="PK710_11925"
     CDS             6503..7675
                     /locus_tag="PK710_11925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOT10475.1"
                     /translation="MDLAKCGSGDTSEARHGCVGDRFRYAGTVRGARSLGFLIPIAAR
                     VYSRIPQETLGLGTLTSAVLRAVDDRNGWFDLADTDAFRLCHGEGDRVPGVVLDRYGT
                     IVVARFDGEAISTWSEMLLGSLWPALKARGFTALAVRDPTRGNASVRVFAGERIPDTI
                     TVRERGMKMVVDLMKGQKTGAFLDQRENRRRIRALASRARVLNLFSYSGGFSCAAALG
                     GAAHVTSVDIAHAAHAIAHETFRQNGIDPNAHSFITADVFLFLERAIDSKERFDLVIS
                     DPPSFAPNEKSLPKALHAYRRLHEACLRVVAKGGLFCAASCSSHVTLDAFIGTLDDEV
                     LAGRPFRIVETFGQPADHPTVPGWPEGRYLKFVVMAEAAARQASPSHYRDENPCAQ"
     gene            7831..>8785
                     /locus_tag="PK710_11930"
     CDS             7831..>8785
                     /locus_tag="PK710_11930"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT10476.1"
                     /translation="MELTLFIDHQCNLRCTYCYNGEKFTRPMSLQTAKKAVEVALSLG
                     STPFFDVSFFGGEPMLRMDLIRDIVEYCHERLQSLRPKPPLRFVMNTNGTLLTDDAID
                     FMRSCGNFSVFVSLDGDKAVHDRFRIHASGQGSFDDVVAGIHRLRKAKIRYQLVGVIT
                     AQTASSLGRTARTLMSLGGDKAVLAPNFRDEWTDASIAALHQGLSDAGRVWMDVFRSG
                     KKFALEPLHTKILTHLKGGIPCPSRCRIAGSELCVSPSGKIYPCAQMVGEDDNDSLVV
                     GHVDEGMDMDRVLELQRKKDRVETVCEPCALRDRCQSHCGCR"
BASE COUNT         1893 a         2658 c         2365 g         1869 t
ORIGIN      
        1 cgatgggccg atttttcgtc catcaaggcc aatcatctct cgataacttg gacgcactgg
       61 caccgtggat cctacggttg cgtcgtcagg cgcgaactcg ggcccccacc cggctcatcg
      121 ctgccgaaaa actgcttgtc gatgcgattt tcgacgtctc ccagcatccg gaggaacccc
      181 tccgatggca gcagattctc ctttcccttg ccaatatcga aagcgtattc gtctcaggaa
      241 ccggttttgc cgcgggccct gtcccgccgt tgaatccgaa atgggtgcag gccgccgatg
      301 acggatcccc ggaatttcgt ctggcggttg cttttgcatt acaacgtatt cgtcaaggaa
      361 agcccgatgg agtgcgtcgc cattggcttc ccctcaatcg acaacaacgc ttcgaaacca
      421 cgggagacag gggatcggct cttgctcggc gcccggatgt cgtgatgttc ggaagggacg
      481 gcatcggcga cgctttagct gtggtcgagc gtcgcctcgt ggaggccagt caatccggcc
      541 aacggcaatg gatgctcgaa gcagcgcgac ggacggatgc ttctctctcg gaccttaccg
      601 cactactttc cggggaagtc gatatcaccc ataccatgaa tctggccaaa gccttgatgg
      661 ccattgatcc ttatgaatgg cagagacagc ccgctcctcc atcgcccccg tcgtttgagc
      721 aacgctggcc tgacgactcc tgggtagcga tccgactggc attgctcccc tggcctttgg
      781 accatgtcca gattccaaca gatcccgcca ttgtgcgtcg gctttccgcg ggcgataccg
      841 catccgccgt gcgattggct ttgcagcggc ttcgtgccgc gggattgcgc tgtcctttct
      901 cgattcctgt aggaaatccg gacggtcgcc tgtggggcgc cgcccttgct tttcccattt
      961 ctcttcgttc ggcacgccaa ttggcacgcc gcttcgatcc ggcttccacc attacggaga
     1021 tgatgccatg agtgttgatt ttcagaccct ctccacccta ccacggttgc tcttttccat
     1081 ccctcttgcg ccattacaag gcagtcgatt tcaaccgacg ggttttccca gcctcggagc
     1141 ggctacgttc cagacctcgg agggaacgag cctgctggtg gaaagcgcgc agagcatggc
     1201 caaccgattg gaaatgacga tctgggacga ggcaagccag gacgtcatcg agaccgtgaa
     1261 aggaatctcc cacgtaaaag tcatgcggga aggcaaattt ctcaccgata ccattttgga
     1321 agcccatcga ttgaatagcc cctacttgct agtggagagc agcaaggcgt ttttcaatct
     1381 gctcaaagag gaactggggg gcctggaaac aggaccgatc aaccgccgaa tcctagcgga
     1441 aacgctcttg aaattcgata tcggttcgct gctgcacggc gttttcctag caaaaaagga
     1501 gttggccggc ggaagactgc ggatagcgcg agccatttcc gcatttatcg aagcggatgg
     1561 cgccaaagtg gccgcctccg gaggggtcaa gaacgaccat gtcaatcctt cgggggatac
     1621 caaggccgga tttggtaacg ttcctttcgc tcgcgacgag ttcacggccg acaccatcac
     1681 gctgtacgtg aacatcgatc tcgcgcaaat tcgaggctat ggcctgggaa aagacgtcga
     1741 ggaactcctc atcgctttgg cccttttcaa actccgtcgc ttgctttcct ccaatctgcg
     1801 tctgcgcacg gcctgtgact tgaaagccac cccgacgacc atcgaagcca gcgaacccaa
     1861 aggattcgtt cttcccacgg aatcggatct ggcaacgctg gtaaaagaaa aaatccaggc
     1921 cacgaaagat aaaatgaagg tatccgaagc gaagtacgat gaaccgatga agaaaggaaa
     1981 agaggagcct gaggacaaac catcggatga ccgctcggaa aaccacgagg attgaccatg
     2041 gtcaccatcg tattccgatt tcctgggggt cggtaccatg ccacgcccac aggtcatcac
     2101 gtgaatgaag gatggattga atggccgccg tcgccatggc gcgtgcttcg ggccctcatc
     2161 gctacagggt ttaccaagct tcattgggtc accatccctg accctgcgcg cagcctcatc
     2221 gaaatgctgg cagacacgct tccagagtac gtccttcccc gcgccggcac gggtcatacc
     2281 cgccattata tgccactcgc ctctatcgac aaaggacgag agaaaaccac tctcgtcttt
     2341 gatagctggg ctgatgtagg gaaaggacag ctcgcaatcc gctggaacgt gattcttccc
     2401 gaagaacaaa cccagcttct acggaacctt gtggaatcca tgagctatct cggacgagcg
     2461 gagagctggg tggcgggatc tcttgccgac gattcctgga gcccccctcc cgattcgcta
     2521 cgagcttatc cctgtgaggg ttcgaatcgc cccggtgctg gatgggaaca agtagcattg
     2581 ctcgctccgg agcccgcgaa cgaatacagc cgctggcggg aatcgcagat ggcttgtatg
     2641 ccgcaagtca ctgaaaagcc taagaaaaaa ggcaaaaaga agagtacgaa acacgaatcg
     2701 gacatcccgg aagacctgat agcctgcctg ctggtcgaca ccacatggtt ggaagcatcc
     2761 ggctggaatc aggctcccgg atccagaaaa gcgctgtact ggcgtccttt tcgagccctc
     2821 gaacccgggg ctcccttgcg tcgaagagcg atgcacaatg ctccccccgt cgaagcgatg
     2881 ctcctcgctc tatccacgcc ttcgggaaac atgcatgcgt tgcccccaat cacacgtgtc
     2941 cttccgcaag ccgaactcct gcatcgttcc ctcgtcagtc atatcgctcg cctgtctcct
     3001 tcccatcacg gaaccctcac aggaaagtcg gaaaacggct ctccgcgccg aggccacagc
     3061 catgtgcatc ttcttgccct ggacctggat agcgacggcc acctggacca ttttttgttg
     3121 tgggctccgg ctggactcga cgcggcctgc caggaagcgg tacgagctac ccgaaaaacc
     3181 tatatgaagg gaggagtggg ggaattgcaa ctggctatca gcggggcagg ctcccttgga
     3241 gatctacgca cggctcccgg gccggaaggc aaaaacttac tccaatccat tggagtgtct
     3301 cccgtttgga cttctgccac gcctttggtt ctaccacgac acctcaaatc caaagggcgc
     3361 catacgctgg aaggccagat tcaagaggag cttgcgtccc gagggctgcc ccccgctaca
     3421 agcatcgaaa ttctatcgca acaacaagat ttcctgcgcc gttttcctca cttcgtccgc
     3481 gttcggcgtt ccggtccccc tccccccgcc gaccagggtt atgcgttaag cctgagtttc
     3541 gaccaaccca tcgctggtcc cctatgcctc ggctatggaa gtcatttcgg tctcggccgg
     3601 ttcgtaacct cctcccctcc cccgtcatag gcgcttcaag aagagagagc ggatacttgg
     3661 acggcgtggt gccacgtcgg cccatatgct caaccgcaca aggggtgttg ggtcgcgaca
     3721 agccggcttc gttcaaggca acatccggta ccacacgggc gggtttgaaa cccgccccta
     3781 catgcgaatg aatgccgcat ggaacgccga cgaatccgga caaccaatcc gctccctcag
     3841 ccatcccctc atttttcccc gcgcctttcc ttcaccctgc gacagatgga aacaagcacc
     3901 taccatcacg aggttgaatt ttcactcgcc agcaaacgaa gatagttctc tcgcgccgca
     3961 atcagcgtgc gacgcatatt gcgcagggtc gctcccgctt gccgttgcga cgcgtggcct
     4021 agcaaggcca actccacgat actgattcct tcatggatat ccgccgcctg cacttccgtc
     4081 cgcccttccg aagccgccct gcaccgcgca tatcgggaaa tcacaatggt cgatagcgcc
     4141 aaatcgatga tgacctccat caaaaagcgc ggatcgcgga aaatttgcct tcgctcgagg
     4201 accagccgca gcaaccggtt gatcggacgc gctttttccg tccccaaaaa ccgttccacc
     4261 ccgtccacgc tccccagcgg cacgggattg cgaatataca atacatggac cgagccccga
     4321 cagagtatcc acttgagttt cgtccatagc acccgcagcg attgcatcgc agaccgaggg
     4381 tcaatccgat gatgcaacgc cacccgcgac gggtagctca atccccctag cacgtaggca
     4441 tggagcgtcc gcgcttgaaa cggcagactc gtgctgagtt gaaaacgaaa tagctgcgtt
     4501 tcatccgcgc gggccaacgc ttccgtcacc cccgcatcgt cgggcttctc cgacgatccc
     4561 ggcagcgcat cttcgaacgc tttcgtccaa tcatagagtt cagacagcaa ttcaagggga
     4621 ggcgcctgcc gttcgagccg ttccaaccac ttatgaacga cacgcatata ttgactctgg
     4681 gtcaaaatac gcccgtgtcg ggtcgccatc gccttggaca tcctctccga tccccctttt
     4741 tgggatagct tgtcacgcaa acgctgtacc gtaacaaggt ccccaattcc atcgcgaatc
     4801 gaagggcaaa gctgggacat ctgagccacg agctggtctt tttcgttgct cacgaaccca
     4861 aaggggaaag actggcaagt ttgcggcaaa aaatcgtgac ccagcttctt ttgcatgctg
     4921 cataattcgt cgtcatcgag aaaaacacat tgcggcatct ggtcgacgtc acgaatgggc
     4981 accacgcctt tggcaatcca cgccatccca gcgtccccca cccgttcccg aacccaaggt
     5041 tccttcgtaa aacgctcaat ctcttgcgta ttcagcggaa tggccacggg cagcctgcaa
     5101 caacgagcgt tgcaatcgcg acaatcaaat cgctgcgtgg aatcaaatac tatcttggga
     5161 gccatgaact atgccacaaa tgccatcagt tatcgtcgac caagccgcga gccaaacggc
     5221 ccttggaaac atcacggatc gctcggtaac accgtaatga ccatcgaatg gaaccaatcg
     5281 ccctgccgcc cgggattcgg ccccgccacc actaccgtat agtcttttcc gtcctgcaaa
     5341 gacgacaagg acaatccacc gcggtcgaga gcatcggtca acaacgattc ggccacgggc
     5401 gtcatcgagt tatccacaaa cacgcgaagc aggttttcct gggaactggt tcccagattc
     5461 gccacgctga gttccttcga tggcggtctc ggcgctattt ttccgggtga aaggctcgcg
     5521 gcgagcaccg tcgccttata aggaaacgcc gtaacgtggt cgagacccag tttttctgtc
     5581 gccttcgaag ccgaaatcac ctgcacgccc atcgcaccgg ccggtgaaga ccgatccatc
     5641 gaagccacca tcatcgtcac atttcctttt tccgatgaat aatcatctcc gcatacgctc
     5701 tgcgtgtccg ggtcctctac ctgcatcgga gcgcaacccg cggcaatcat cagcacgcta
     5761 cgcccccgcg acaatgttcc cgcgggtacg acgggcagaa tagtccgcgt aattttttcg
     5821 gggacagcct ccaattcgtc acaacgacgg ccttggatgg tttcgacggc acccgtatag
     5881 acgaccggct ggatgtcttg cgcggcaagg tctgcgcctt cgaggctttg ggcggaaaac
     5941 gcgctaccat acagcaagcc aagggtgtcg gatggcatgg caagcacggg gaggggaacc
     6001 atgctcgttg cttttccagc caaaaagcaa agccgaagcg cccgtgcatt cggaatccca
     6061 tgaaaaaacg taaaaaccga cggcccatca tgtacgaaac tgctatcggc cggcgcgaca
     6121 tcgtccatgg aagcctcgat ttcgcgatcg ttcatattac catccgcacc tgatgcatcg
     6181 ttcgagacgc tggcgtccac cacgacccca ggcgaggcat cgttggctac gagcgcgctg
     6241 gtatccggtc cactgcatcc gcagatcgac agagcaaccg tcacgatggt tgcgaccatt
     6301 cgcatgttct gttcatagcg cgccctcggc cacggatcga gagttgatgc atgctctttg
     6361 gtttcatcct gctgctttcg aaccaaagag tgtgcaacga taggctttgg ggattcccac
     6421 agatgaacgg aaaagcatta cctgcacctt tgcgaatttc gatcgacgcg cgttcggccc
     6481 acgtgattcg gcgtgggcat ccgtggatct ggcgaagtgc ggttcgggcg acacctcaga
     6541 agctcgacac gggtgcgttg gtgaccgttt tcgatacgcg gggacagttc gtggcgcacg
     6601 gtctttggga ttcctcatcc ccattgccgc ccgagtatat agcaggattc cgcaagaaac
     6661 gttgggtctt ggcacattga cttccgccgt tcttcgggcg gtcgatgatc ggaatggctg
     6721 gttcgatctt gcggacaccg atgcgtttcg gctctgtcat ggggaaggag accgggttcc
     6781 gggggtggtg ctcgatcgct atgggacgat tgtcgtggcg cgattcgatg gagaagcgat
     6841 cagcacctgg agcgagatgc tgctcggttc gttatggccc gctttgaaag ccagggggtt
     6901 tacggcgctc gccgtgcgag atcccacccg cggcaacgcc agcgtccgcg ttttcgccgg
     6961 ggagcggata cccgatacca tcacggtccg cgagcgcggc atgaaaatgg tggtggactt
     7021 gatgaagggg cagaaaaccg gtgcctttct cgatcagcgg gagaatcgca ggcggatccg
     7081 cgcgcttgct tcgcgggcgc gagtcctcaa tcttttcagt tattcaggcg ggttttcttg
     7141 tgcagcggcg cttggagggg ctgctcatgt cacctccgtg gatatcgccc acgccgccca
     7201 tgctatcgct catgaaacgt ttcgacaaaa cggcatcgat ccgaacgctc attctttcat
     7261 tacggccgac gttttcctat ttctcgaacg agccatcgac tccaaggagc ggttcgatct
     7321 ggtcatcagc gaccccccat cgttcgcgcc taacgaaaag tctctcccca aagccctgca
     7381 tgcgtatcga aggttgcatg aggcctgcct tcgtgtggta gccaaaggcg ggttattttg
     7441 cgccgcttcc tgctccagtc acgtcacgct cgacgccttt atcggcacgt tggatgatga
     7501 agtcctcgcc ggacgtccgt ttcgcatcgt ggaaaccttt gggcagcccg cggatcatcc
     7561 tacggttccc ggctggccgg aagggcgtta cctcaagttc gtggtcatgg cggaagccgc
     7621 ggcgcgccaa gcttcaccat cacactatcg cgatgaaaac ccctgcgccc agtaatgctc
     7681 cgatcgatat tcgaccttgg gctgtcatga gcggttgtaa tcgttcgcgc gacttgtccc
     7741 catcgacgat ttcgatgggc ttgtggtcgc gaacgtcctt cggaaatgtc aggatgcatc
     7801 ctgctttctc gatccggagg caggacctcg atggagctga cactatttat cgatcatcaa
     7861 tgcaatcttc gatgcaccta ttgctacaat ggagaaaaat tcacgcgccc catgtccctg
     7921 caaaccgcca aaaaagcggt agaagtagcg ctttccctcg gtagcacacc ctttttcgac
     7981 gtctcgtttt tcggcggaga acccatgctc cgcatggacc tcatccgcga tatcgtcgag
     8041 tattgccacg aacgcctgca atcccttcgt cccaagccgc cgttgcgttt cgtcatgaac
     8101 accaacggca ccttgctcac ggacgatgcc atcgacttca tgcgatcgtg cggcaacttc
     8161 tcggtgttcg tatcgctcga cggcgacaag gcggtgcatg atcgatttcg catccacgcg
     8221 tcgggacaag gaagtttcga tgatgtcgtc gcgggtattc atcggttgag aaaggcgaaa
     8281 attcgctatc aactggtggg agtgatcacg gcgcagaccg cctcttcgct cggacgaacg
     8341 gctcgtacgc tgatgtcgtt gggcggggat aaggcggtac ttgcaccgaa ttttcgggat
     8401 gagtggaccg atgcctccat cgcggccttg catcaaggat tgtcggatgc gggacgagtg
     8461 tggatggacg tatttcgttc cggaaaaaaa tttgccctgg agccattgca taccaaaatc
     8521 ctcacccatc tcaaaggcgg tatcccctgc cccagtcgat gtagaatcgc aggttcggag
     8581 ctgtgcgtga gcccttcggg aaagatttat ccttgcgcac aaatggtggg agaagacgat
     8641 aacgacagct tggtggttgg ccatgtcgac gagggcatgg acatggatcg agtgctcgaa
     8701 ttgcaacgaa agaaagaccg agtcgaaacg gtttgtgaac cctgcgcttt gcgtgaccgg
     8761 tgtcaaagcc actgcggatg tcgac
//
LOCUS       DAOSOR010000195         8779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_7970_length_8779_cov_4.375745, whole genome shotgun sequence.
ACCESSION   DAOSOR010000195 DAOSOR010000000
VERSION     DAOSOR010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8779
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7970_length_8779_cov_4.375745"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..325)
                     /locus_tag="PK710_11935"
                     /pseudo
     CDS             complement(197..325)
                     /locus_tag="PK710_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740562.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
     gene            complement(540..1430)
                     /locus_tag="PK710_11940"
     CDS             complement(540..1430)
                     /locus_tag="PK710_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT10477.1"
                     /translation="MVLSLLVEKIGAYCAAMLLEGKVALVTGGGRGVGRAVAMAMAAQ
                     GARVVVNDLGCGLDGSGEDESVADEVVEQLRSTGAEAVASHDDVATGLGAKAAVQTAI
                     DAFGDLDILVYSAGISRDKTLLKLDEASWDQVMGVGLKGAFLTMQAAARAMISFGHGG
                     RMVVMSGLSGYLGNFGQGNQAAALAGVHGLMRTAAIEWQKHKITVNAVAPLAKTRMTE
                     ALPLLQGFENLTVEHVVPAVLALTSDLCGDRSGTVLAATGARIYSFRFVESAGKFKDE
                     AAGVFTPEEVDEHWPSIVKI"
     gene            1485..3254
                     /locus_tag="PK710_11945"
     CDS             1485..3254
                     /locus_tag="PK710_11945"
                     /inference="COORDINATES: protein motif:HMM:NF013151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10478.1"
                     /translation="MVHDKIAVVDFDGFHAHLIATQIRRFHVLSEILDPSTPPSALGA
                     YRGIILSGCFPTPGQPVNPAWLALPLPILGLGPAQTERGKSHQRRSLALRFTSDCTLT
                     GHLPHEMAVDIDVPPLALPEGFREIGVIIQPDGTMRHNAAIAHQSHKHYGLPLFPGDG
                     PRDDSLWLENFVLRICGCQPTWTIEQVLEEQIATIRQNVGDRGVFLLVSGGVDSTVCA
                     SLLGKALGPDRIHLLHIDNGMMRKNESSHVIAEFKRLGLARHLHFVDASDRFLSNLAG
                     VIEPEKKRLIIGNTYLEVFEAEAERLQLSDMLLGQGTIYPDTVETGSSGAPAVKTHHN
                     RVPLVEKMIAQGRVIEPIRDLYKVEVRELGERLGVDPSLVWRHPFPGPGLGVRTLCSD
                     GTLPQGHEQARWQPLIDAQTGPVGMHGLLMPIRSVGMTDHQRTYEQPVFLWGKATEEK
                     IIRLSENVFRQIPGINRCVVDLTGKGASRMQPLAASLTRQRLDLLREADEIVMKGLAH
                     HGLERAIWQCPTVLLPLSIDGKGQELVVVRPVYSERAMTARPAWLPEPLVSELLAEIL
                     ALPGVSGLALDVTPKPPGTIEWE"
     gene            complement(3681..5099)
                     /locus_tag="PK710_11950"
     CDS             complement(3681..5099)
                     /locus_tag="PK710_11950"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT10479.1"
                     /translation="MWYRNSFLLLVEGSVVLAWVGGCRLGESPDDFSKGTGEPKEAGP
                     QGGFGGSGASNDSIRCEKTPTGCEPFGGHGERLFGCCDGDLRWWCEPVEGIWTLRSED
                     CGQKGLNCGYDAKEAMLRCVSGAGGAGGATGGASGATGGAGGATGGAGGATGGAGGAT
                     GGSGGSTIYGVPGPSCEGMTGKECQGKNCCSSILVPGGVFPMGRSENGSDACSVGMQY
                     CKTDEQPEHDVTVSDFYLDEFEVTVGRFRKFVDQFDGTLPSDGAGEHPLIPGTGWHST
                     WNHLMPSSKADWQSHLKCDPVYPTWRDTKGDTEQHPINCVSWFDAFAFCVWDGGRLPT
                     EAEWEYAAVGGIDNRLYPWGSVEPSPQLAVFDCQYDGKTGCSFADIAKVGSKPAGAGR
                     WGHKDLAGNMVEWVLDVYDEGWYSAEGNKCNNCASFSEGESSVCRGGCWSYVEPSWLR
                     GAVRLSFSSVHGLFPVGFRCAR"
     gene            complement(5789..6700)
                     /locus_tag="PK710_11955"
     CDS             complement(5789..6700)
                     /locus_tag="PK710_11955"
                     /inference="COORDINATES: protein motif:HMM:NF006077.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOT10480.1"
                     /translation="MTAAFEYGQAFSRNIGWITEQEQQVLRETRVAIAGMGGVGGSHL
                     LTLTRLGIGRFSLSDFDRFGVENFNRQAGARLSSVGRPKLETLVEMALDINPHLDIRT
                     FPQGIGAHNVDHFLSGASVYVDGLDFFAMDARRAVFSACHRLGVAATTAAPLGMGTAV
                     LNFLPGKMSFEQYFRLDGLPEAEQYLRFLVGLAPARIHQGYLMDPSRVSLQARSGPST
                     PMACELCAGVAATQVLKIVLKRGRLLAAPWGLQFDAYENRLATTWRPGGNRHPLQRLA
                     IAVGRWLLAGHLKPARQALPPSTEPQT"
     gene            6862..7545
                     /locus_tag="PK710_11960"
     CDS             6862..7545
                     /locus_tag="PK710_11960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT10481.1"
                     /translation="MASIVAENVSKSFRQGREFVHVLEDVSLSVDPGEVVALEGPSGS
                     GKTTLLSILGCILTPSKGRVVIDGHEVLASDGSKLAEVRKRFIGFVFQQYNLFAALSA
                     LENVELALNIKGVRGPRARQAAQKALEDVGLGDRMGFLPRDLSGGQKQRVAIARALVG
                     SPSVLLADEPTANVDSRAGQAILEIFRTLAKRDARALLIVTHDPQVRTVTDRVLCIRD
                     GRLTSESGT"
     gene            7548..8465
                     /locus_tag="PK710_11965"
     CDS             7548..8465
                     /locus_tag="PK710_11965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT10482.1"
                     /translation="MGMRRAFVWAGGGMAVVTAAVAGWLLFGGPDVWSSAEAAAAQSA
                     PAMEPTPAIRAEGRVSTYPGDQVHLGTELGGTITRLHVREGETVKKGDILVEFDDTTL
                     RAALGEAKAGVHAHNARAGFHVKETQRLKTLSDSGAVPPRELDRAKSERNATFAETAV
                     ALSTVKRIEADLAKTVLRAPIDGTIIACSVEQGETLPPMAQLLTIANLDRLRIVAEVD
                     EFDAARVELGSEATITAEGLDTSWDAKVEEVPGALVPKGLKPRDPARPVDTRVLLVKL
                     GLLQPTPLKLGQRVEVRIHLPPTHASEGR"
     gene            8470..>8779
                     /locus_tag="PK710_11970"
     CDS             8470..>8779
                     /locus_tag="PK710_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10483.1"
                     /translation="MLERLRDALIDRRIRSKAAIHHTPLDRYRVRIARDVHQHEDAFR
                     LLHVAYAFQGIENIRSDKLRITPQHVLPEATVFVAYEGEQCVGTMTVTLDSPAGIPVE
                     K"
BASE COUNT         1876 a         2844 c         2306 g         1753 t
ORIGIN      
        1 tccaagcctc ttcccattga ggtgctgtct gaccgggtaa tggatcatac cctacgacac
       61 gcttctttgt tgttcgaatg tctcgagcgc cgcgacaggg caaaccgctg cccggcacac
      121 cgtggatgac ttgccctgcc ttgggtggat tccatgactt tgacatcacc gaatactctg
      181 ccgtcacccc catctgcccc cacggataca gccaattttc atcccctccc actgccgcat
      241 attcccactg cgcttctgtc ggaagcctgc ccccgtccca cgcgcaatac gcaaatgcct
      301 cgtaccagct tacgtaattg atgggatgtt gcgacgtatc cgtttccggt tcgcgccaag
      361 tggaaatgga ctgtcctaca acaccatacg gtttcggaag ccacatcgat atcggctctt
      421 ttgccctgcc ggaaaacctt accgaaacaa gtcaatccca gtccccgaaa acccgatggc
      481 gtcgatccac ccccccatcc ttctgccaag cggtggatca agaaaatacc ttatgtttct
      541 catatcttga caatggatgg ccagtgctcg tccacctctt cgggcgtgaa cacccccgcg
      601 gcctcatcct tgaacttgcc tgcgctttcc acgaaacgaa acgagtaaat ccgcgcgccg
      661 gtggccgcta gcaccgtccc cgaacgatct ccacacaagt cgctcgtcag cgccaagacc
      721 gctggtacca catgctccac cgtcagattc tcgaaccctt gcaacaacgg caacgcttcc
      781 gtcatccgtg ttttcgcgag cggtgccacc gcattgaccg taattttatg cttttgccac
      841 tcgatcgcgg ccgtacgcat caacccatga acacccgcca gggcggccgc ttgattgccc
      901 tgtccaaaat ttcccaaata ccccgatagg ccgctcatca ccaccatgcg ccccccatgc
      961 ccaaacgaga tcatcgcgcg ggcggctgcc tgcatggtca aaaacgcccc cttcaacccc
     1021 acccccatca cctgatccca cgatgcctcg tccagcttca gcaacgtctt atcccgcgaa
     1081 atccctgcac tatagaccaa tatatccaga tccccaaacg catcgatggc cgtctgcacc
     1141 gcggctttgg cccccaaccc ggtagccacg tcatcatggc tcgccacggc ctcggccccc
     1201 gtcgaacgca attgttccac cacttcatcg gccacgcttt cgtcctcgcc ggagccatcc
     1261 aagccacacc ccagatcgtt gaccaccaca cgcgccccct gggcagccat cgccatcgcg
     1321 accgctcggc ccacgcctcg ccctcccccc gtcaccaacg ccacttttcc ttcgagtaac
     1381 atggccgcgc agtatgctcc tattttctcc acaagcaacg aaagaaccac ccctccgaac
     1441 ctcttcgtgc tatcccatcc ccaccgccag ccccggatat gcccatggtt cacgataaaa
     1501 tcgccgtcgt cgacttcgat gggttccatg cccatctcat cgccacccag atccgacgtt
     1561 ttcacgttct ttccgaaatc ctcgacccca gcacgccccc atcggcgttg ggcgcctacc
     1621 gcggcatcat cctatccgga tgcttcccca cccccggcca gccagtgaat cccgcctggc
     1681 tcgcgttacc ccttcccatc cttggtctcg gaccagccca gaccgaaagg ggaaaatccc
     1741 atcagcgccg ttcgttggcc ctacgattca cctccgattg cacactcacg ggccatctac
     1801 cccatgaaat ggccgtggac atcgacgtgc cacccctcgc tttaccggag ggatttcgtg
     1861 aaatcggcgt cataatccaa cccgacggca ctatgcgtca caacgccgcc atcgcccatc
     1921 aatcgcataa acactatggt ttaccgcttt ttccaggcga tggcccccgc gacgatagcc
     1981 tttggctcga gaatttcgtt ctgcgaatct gcggctgtca acccacctgg acgatcgaac
     2041 aggtcctcga agaacaaatc gccacgattc gccaaaacgt tggggatcga ggtgtatttc
     2101 tactcgtttc cggcggcgtg gattccacgg tttgcgctag cctgctcgga aaagcactcg
     2161 gtccggaccg tatccacctg ctgcatatcg acaacggtat gatgcggaaa aacgagtctt
     2221 cccacgtcat cgcggagttc aaacgtctgg ggctggcacg ccatcttcac ttcgtcgacg
     2281 cctccgaccg ctttttatcc aacctggcgg gcgtgatcga accggaaaaa aagcgtctca
     2341 tcatcggcaa cacgtatctc gaggtcttcg aggccgaagc cgaacgtctg caattatcgg
     2401 atatgctgct gggccagggg accatttatc ccgatacggt cgagacgggc tcctcgggcg
     2461 cccccgcagt caaaactcac cataaccgcg ttcctctggt tgaaaaaatg attgcccagg
     2521 gccgcgtcat cgaaccgatc cgtgacctgt acaaggtgga agtcagagaa ttgggagaaa
     2581 ggcttggcgt cgatccaagc ctcgtctggc gacatccgtt tccaggaccg gggctcggcg
     2641 tccgaacgct ttgctccgat ggcaccctgc cccaagggca tgagcaagcc cggtggcagc
     2701 cgctcatcga tgcacaaacc ggcccggtgg gaatgcatgg cttgctgatg cccattcgat
     2761 cggtgggaat gacggaccac cagcgaacct acgaacagcc tgtttttcta tgggggaaag
     2821 ctacggagga aaagattatc cgcctgtcgg aaaacgtgtt tcgtcaaatt ccaggcatca
     2881 atcgctgcgt ggttgacctc accggaaaag gcgcgagccg gatgcaaccg ctggccgcca
     2941 gccttacgcg ccaacggctg gatttattac gcgaagccga tgagatcgtc atgaaagggc
     3001 ttgctcacca tggactggaa cgcgcgatct ggcaatgtcc tacagtgcta ttgcccctgt
     3061 cgatcgatgg gaaaggccag gaattggtcg tcgtgcgccc cgtgtatagc gagcgagcca
     3121 tgaccgcacg tcccgcgtgg ttgccggaac cgctcgttag cgagctacta gcggaaatcc
     3181 tggcactgcc gggggtgtcg gggttggcgc tggatgtgac gcccaagccc cccggtacga
     3241 tcgaatggga ataggggcgt aacgcttctt tgccacgcag ggcgcacgcg acgatccgca
     3301 ggcgaccatc ctagccgagc agcagcctat cctggctggg ctaaccatga cgcacctcgc
     3361 acagggtgag aaaccccggt agcccgcgag catcaccccg cagcgtacaa ataactgtat
     3421 ttattgatgt ttttatcagg attgaccaaa atggactcaa ggactaggtc aaagtcaaag
     3481 gccaggggat tccctgctcc tatccctttg caattcagga aaaatgaggg gatctggtag
     3541 gaggcgaccg agcctacggc tcgagcgcga tccagacgtt gtctgacagc ttcgggattc
     3601 tgccacgtcg acgcgaatca tgtcattccg aaggacaagg gggcaacagg catataaggt
     3661 cgcgggttcg agcaagaggg tcatctggcg caacgaaacc cgacaggaaa gagcccatgg
     3721 acagacgaaa aggagagacg aacggcgccg cgtagccacg atggctcgac atagctccaa
     3781 cagccgccgc ggcacacgct gctctcccct tctgagaaac tggcgcagtt gttacacttg
     3841 tttccttctg cactgtacca accctcgtcg tacacatcca gtacccattc taccatattg
     3901 cccgcaaggt ccttgtgtcc ccaccgacca gcgcctgccg gtttggatcc cactttcgca
     3961 atatccgcaa aggagcaacc cgttttccca tcgtattggc agtcaaatac cgcaagctga
     4021 ggactcggct ccacactgcc ccagggatac agcctgttgt cgatgcctcc gaccgccgcg
     4081 tactcccatt ccgcttccgt cggaagccga cccccatccc acacgcaaaa tgcgaaggca
     4141 tcgaaccaac tcacgcaatt gatcggatgc tgttctgtat cgcctttcgt atcacgccac
     4201 gtgggataga ccgggtcgca cttcagatgg ctctgccaat ccgctttgga agacggcatg
     4261 agatggttcc aggtgctatg ccaacccgtc ccgggaatca gaggatgctc gccggctccg
     4321 tcggacggta gcgttccatc gaattggtcc acgaacttgc gaaatcgacc taccgtgacc
     4381 tcgaattcat ccagataaaa gtccgatacc gtcacatcat gctcgggctg ttcatcggtt
     4441 ttgcaatact gcattccgac tgaacatgca tcactgccgt tttcgcttct gcccatcgga
     4501 aacacgccac ctggcacgag aatgctcgaa caacagttct ttccttgaca ctccttgccc
     4561 gtcatccctt cgcaggaggg ccccgggacg ccgtatatcg tggagcctcc gctgcctcca
     4621 gtcgcgccgc ctgctccacc ggtcgcgccg cctgctccac cggtcgcgcc gcctgctcca
     4681 ccggtcgcgc cgctcgctcc accggtcgcg ccgcctgctc cacccgctcc ggacacgcaa
     4741 cgcaacatgg cttctttcgc gtcgtatccg cagttcaatc ccttttgccc acagtcctcg
     4801 gaacgaagcg tccagattcc ctccaccggc tcgcaccacc accgtaggtc gccatcacag
     4861 caaccgaaca atcgctcccc gtggcctccg aagggttcgc agccagtcgg tgttttctcg
     4921 catcgaatac tatcgttcga ggctccgcta cctccaaatc ctccttgagg accggcttct
     4981 ttcggttccc cggttccttt ggaaaaatca tctggggatt cccccaaccg acatccgcct
     5041 acccaggcca atacgacgct tccttcgacc agcaacagga atgaatttcg ataccacata
     5101 aatttacacg aaaccggcga gttgataggg cgtgttgttt tcccaatcat accaatcacg
     5161 agatcgtcac ctgggatatc cccggcccat gccaatcacg acgaacaata gcgtagcccc
     5221 gccgtgttgg caatgtcgca cattgcggtc cgatagggcc agggcccacg gtcaccgccc
     5281 acgcgaaacg gtcaccaaac tgggctgaca atgacgttcc tcgcggggaa tgaaggcctc
     5341 gccgagcctc cgcagaaggc ctcgtggccc gcgggtatcg tcgtcacact gcacgcaact
     5401 agacgtattt acaggtgttt gtataagggc tggtcatgat ggggctcagg tgctggtcga
     5461 cagtgacact ccttcgtttg ctcttcgggg cgaacggtcg gggcaaagga ctgttatgat
     5521 gaaagaggcg cttgccttgg cgtgccaggt tgccgccatc agggccctga ccttgtcaaa
     5581 caaagcgtct ggttcgaata cccagcgtca cgaaacccga gctattcgtt ccgagcgaaa
     5641 ggcgggtgga ggccaagcat aaaggcgtgt taaatcagta tgttgacact atccccgggg
     5701 aatgaccgac gagttgcgga tggacgccat gtaccgcctt gcggaaggaa ggtctattat
     5761 tccctcccct cgacggagga acgcaacctc atgtttgcgg ttcggtggat ggcggcaagg
     5821 cttgtcttgc gggtttgagg tgaccggcga gcaaccatcg gcccacggcg atggcgagcc
     5881 gttgcaaggg gtggcgattt ccaccgggtc gccaagtggt ggcgagacgg ttttcgtagg
     5941 cgtcgaactg caatccccag ggggccgcga gcaatcgtcc acgtttcagc acaattttga
     6001 gcacctgggt ggccgccacg ccggcacaga gttcacacgc catcggggtt gaaggccccg
     6061 accgggcttg taggctcacg cgagacggat ccatcaagta accttggtgg attcgagcgg
     6121 gtgccagccc cacgaggaat cgaaggtatt gttccgcttc aggcaaacca tcaaggcgga
     6181 aatactgctc gaacgacatt tttccgggaa gaaagttgag cacggcggtt cccatgccca
     6241 gcggagccgc ggtagtcgcc gccacgccca gccggtggca agcagaaaac accgcgcgcc
     6301 gggcgtccat ggcaaagaaa tccaagccat ccacgtacac gctcgcccca gacagaaaat
     6361 gatccacgtt atgcgcccct attccttggg gaaacgtccg gatatcgagg tgcggattga
     6421 tatccaacgc catctcgacc agggtttcga gtttgggcct gccgaccgaa gacaaccggg
     6481 ctcccgcttg ccgattgaaa ttctccactc cgaatcgatc gaagtccgat agagaaaatc
     6541 ggccgattcc tagccgggta agtgtcaaca ggtggcttcc gcccacaccc cccatacccg
     6601 cgatggcgac cctcgtttct cgcaagactt gctgctcctg ctccgtgatc cagccgatat
     6661 tgcgggaaaa cgcttgccca tattcaaaag ctgccgtcat tcacactcct atcgtctcca
     6721 ggaaagcggt gctcccatac cgaccggttt tctgatcaaa aaaacaaaaa tgatttcgac
     6781 gttcgattca gaatctccgc ccgttcaccg cactgcgtcc atagaccgat catcgaccgg
     6841 tccccgcccc acggaatcaa tatggcaagc atcgtcgctg aaaacgtgag caagtccttc
     6901 cgacaaggca gggaattcgt gcatgtcctt gaagatgtct cgctttccgt ggatccggga
     6961 gaggtggtag cgctggaggg cccgtcgggc tccggaaaaa ctacgttatt atctattctt
     7021 ggctgcatct tgaccccttc caaaggcagg gtcgtcatcg acggccatga ggtattggcg
     7081 agcgacggtt ccaagctggc ggaagttcgg aaacgattca ttggttttgt atttcagcaa
     7141 tacaatcttt tcgcggcgct atcggcgttg gagaatgtcg aactcgcgct caacatcaag
     7201 ggtgttcgcg gtccgcgggc tcggcaggcg gcacaaaagg cgctcgagga cgtggggctc
     7261 ggcgatcgga tgggattcct ccctcgggac ttgtccggtg gacagaagca gcgcgtggcc
     7321 attgctcgcg cgctggtcgg ttcgcccagc gtattgctcg ccgacgagcc caccgcgaac
     7381 gtggattctc gtgcggggca ggctattttg gagattttca ggacgttggc caagcgcgac
     7441 gcgcgtgcgt tgttgattgt cacccacgac ccgcaagtgc gcacggtcac cgatcgggtg
     7501 ctgtgcatcc gcgatggtcg tttgacctca gaaagtggaa catgaccatg ggaatgcgac
     7561 gagctttcgt gtgggccgga ggcggaatgg cagtggtgac cgctgcggtg gctggatggt
     7621 tgcttttcgg cggtccggat gtgtggtcat cggccgaggc agcggccgcc caatccgctc
     7681 cggccatgga gcctacaccc gctattcgcg ccgaggggcg tgtcagcacc tatccgggag
     7741 accaagtgca tctgggcacg gaattgggcg gtaccatcac gcgtcttcat gtccgtgagg
     7801 gggaaaccgt aaaaaagggc gatatcttgg tggaattcga tgatacgacc ctgcgcgcag
     7861 ccttggggga agccaaagca ggcgttcatg cccacaatgc ccgagctgga ttccatgtca
     7921 aagagaccca gcgcttgaaa accctatcgg atagcggagc cgtaccccca cgggaactcg
     7981 atcgcgccaa aagcgagcgc aacgcgacct tcgccgaaac cgccgtcgcc ctgtccaccg
     8041 tcaaacgcat cgaagccgac ctggcgaaaa ccgtcctgcg cgcccccatc gacggcacca
     8101 tcatcgcctg ctcggtcgaa caaggcgaaa ccttaccgcc catggcccaa ctgctcacga
     8161 tcgccaatct cgatcgctta cgtatcgtcg ctgaagtgga tgaattcgat gccgcaaggg
     8221 tcgaactcgg ctccgaggca acgatcaccg cggaaggact ggacacctcc tgggatgcca
     8281 aggtggaaga agtgcctgga gccctcgtgc ccaaaggcct gaaacctcgg gatcctgccc
     8341 gacccgtcga cacacgcgtg ctgctcgtca aactcggatt gttgcagcca accccattga
     8401 agcttggaca acgggtggaa gttcgcatcc atctaccccc cacccacgcc tcggagggcc
     8461 gctaaagcca tgctcgaacg cttgcgcgat gcactgatcg accgtcgaat ccgctccaaa
     8521 gcggccatcc accacacccc cctcgaccga taccgcgttc gtatcgccag agacgtgcac
     8581 caacacgaag acgctttccg attgctgcac gttgcctatg ccttccaagg catcgaaaat
     8641 atccgaagtg ataagttgcg gatcaccccg caacacgtcc tgccggaagc caccgttttc
     8701 gtggcatacg aaggggagca atgcgtgggc accatgacgg taaccctcga ctcacccgct
     8761 ggaattcctg tagaaaagg
//
LOCUS       DAOSOR010000196         8764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_7990_length_8764_cov_4.523941, whole genome shotgun sequence.
ACCESSION   DAOSOR010000196 DAOSOR010000000
VERSION     DAOSOR010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8764
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7990_length_8764_cov_4.523941"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PK710_11975"
     CDS             <1..250
                     /locus_tag="PK710_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10484.1"
                     /translation="EGGTGGGGSGNNCDSCMESKCGNEISACENDAQCTGLLSCFDNC
                     EDMDCMEKCVNDNPSGAALLKNVFDCMDKNCQAECADE"
     gene            436..573
                     /locus_tag="PK710_11980"
     CDS             436..573
                     /locus_tag="PK710_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10485.1"
                     /translation="MTNAVRNLLLTQRGIVFVSSHGPRLREIQQQAQQIVQDAFQTAG
                     A"
     assembly_gap    1303..1402
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1403..1624
                     /locus_tag="PK710_11985"
     CDS             <1403..1624
                     /locus_tag="PK710_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10486.1"
                     /translation="AVPTKAAAAAATSVAVPTRAVAAAVTSVAALTKVAAAAATSVAA
                     PTKAVAVVPVPMIARAARWPNAPAKSMLA"
     gene            2241..4823
                     /locus_tag="PK710_11990"
     CDS             2241..4823
                     /locus_tag="PK710_11990"
                     /inference="COORDINATES: protein motif:HMM:NF025803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10487.1"
                     /translation="MTNTVRSLLLTRRGIVFVSSHGPRLPDSLLQALDLELSQLGYIL
                     SSRIRDRLSRCNLEELNEFRSWTITTLAAWLGADQKHKPLFRRFPRGIPSNTVELWWK
                     KILVHYLQADGQTCLFCRRIGTTHVLDPCMHVVCDHCFDGSNYSACPICEHHVNRDSP
                     FFRPTPERPLPSETIVFRLIDLGNDESVETKSLFVSLCERKQPLSPVDRDALFCIVKE
                     RPSQVLSWLPPTIAVRQNIAIVLGTLFSVCESSEVLPHAKRLVTTATDVLRLLAVLSG
                     TDGSLMQETIFRSFANPDDSDELCVHTARRLGMSPSSLIKQKIKVPVRVHRFKVAKLP
                     RPLRRALLGLLESLPADSLVEDMLRHRAYWVWVGEFLHPHEYASRFPNVARAFQIVRK
                     KASDGTPAPRFRTWPSRLEQTLRTGDADAMLSVLSERPGEYARRFDFVLRQARDERAQ
                     RRYAEQLISKINVLSTPWLVAFRSYLPTRIDKAPIRVFWPKGRGAYGVSRPDTRTALP
                     RPIIEPVIQAIDKELLARFGSKRAFAAGWIDEGLRTIMVPMQEATTSRSAVSLPRGSR
                     VPLDRGRLIRLFLHWCEPAHGAWSTDLDLSVGLYDESWRYVGVCSYYELQAKGPDGSV
                     IAQSAGDLRDAPWPDGATEFVDIHADRAQAASVRYVVMVVNRYGGMACSQLERCFAGL
                     MLRDDPMGKHFDPRTVALKFALNSESGVFMPLVLDLRDRVLHWLDVQVAGQLERNNVE
                     TSNTAIQKICPEQMTYFASGVRPCLFDLGLLHAAARCRRVTLRGRNIVEFLRKPEESV
                     NDFHARLVRGEGGVCRSAPADEQEPMFALLYRGDVELPEGSDVYALFREQLTPNLQAS
                     DLLS"
     gene            complement(4899..6017)
                     /locus_tag="PK710_11995"
     CDS             complement(4899..6017)
                     /locus_tag="PK710_11995"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOT10488.1"
                     /translation="MTWPSWDGGRPWARYEENEVETAPFPEATRTCTTDTVPIPDRYW
                     DFFRRLRRRIVSIVLALDRHDAVHQASAMAFWLFLGLIPLAAILGWAVTRFTGTEIRR
                     SVLESLISVTPEPALGLVDEQMHRLGDGTEAVAPLSLLGFLWLASGGVHTAIVAIQIA
                     QAGHARSWLRNRSLALAFVLMLVLVATGSTTLLVLASPTWHRIIRGGFVEAGWMQVVR
                     YGALPTATIIAILGTATFFRFASMSPEADVQKRIWPGAIITGLSWVALSWGFSAYVRT
                     IGRYPIFYGSLAAVALLMLWLWLSSFLVLLGSEVNLQIEGTRKTIAPPSIHWPKGWHL
                     RRPIAASPSKQPTNNNDAAPPAADPIESPSGANEPGGS"
     gene            6148..7122
                     /locus_tag="PK710_12000"
     CDS             6148..7122
                     /locus_tag="PK710_12000"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOT10489.1"
                     /translation="MHRWHRSTRIREGDIRQRPNYVFREGPPIAGFERGKRQALMVGQ
                     GKSKKLCYKPRVEGRFMYADVHTHLTHRAFSGEQDDVAVRAARAGLDCVIVNGLEPRS
                     NREVLTLCARHPHLYPALGIYPVDAVAASIDRASWTYGFEPPDVFDVDEEMDFIDSVA
                     DQLVALGECGLDQYWVRDQAAEQERVLRRFCQIALAHDKPLILHSRKAEQRTLDIVLE
                     MGITRADFHCFGGKLKLAKQIAEKGFYLSIPPVVIRSQAFQRIVQEVPLDRLLTETDA
                     PYMGPDAGERNEPSTIPRAVAAMAELVHLPVEQVRDILRQNCRDLFGV"
     gene            complement(7169..>8764)
                     /locus_tag="PK710_12005"
     CDS             complement(7169..>8764)
                     /locus_tag="PK710_12005"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT10490.1"
                     /translation="QVARLLGAGEVGEVYDVRDMTTGYAYALKLLRPEITQSPDAWAA
                     LCGDAQRAAALESDAIAKAYEFQTEPTLNAPYVLGEYVTFPSLHTIVSEQGPMGLAEL
                     DAILRLVAQSLDLAHRNGIVHRAIKPQNIFANPIDARSWQVRITDFGMGAARAYSPPP
                     PGWTATPGWLSAEQADPSTAPSPTMDVYALGLVAFYALTGRSIFLACRTTPPDLNILW
                     AEMTAPLPPASQRARELGAVLSPTLDSWFAKALAVSPSQRFSSAGEMSHALFQLVGSS
                     QHVPTMRPSGGAAPGPAAPVPEPPSPVIHNYDAQAQPAQGYPQNQQYAPQSLPAAQDV
                     TGAPMPPLPPAAPAAAVMPDTDEIEFKKSPNKLLIPVLIGAAMAFVVLLGIGAWYLFS
                     GKDTPAPAVSSASASAPTVATTVPEAAPPEPEKEASAPEAAPEEKPKDALITFSCTPE
                     CEEIWCDNKKVADFKDGVRLAGGNHICLGKTKGYNPAKDSFTVTPGEDMKRELTLVKT
                     VYSAPPSQPPPAKTCGTLLNPCK"
BASE COUNT         1815 a         2420 c         2539 g         1890 t
ORIGIN      
        1 cgaaggcggc accggaggcg gcggcagtgg caacaattgc gatagctgca tggaatcaaa
       61 atgcggcaac gagatcagcg catgcgaaaa cgatgcgcaa tgcaccggcc tcctgtcctg
      121 cttcgataac tgcgaggaca tggactgcat ggagaagtgc gtcaatgaca atcctagcgg
      181 agctgccctg ttgaaaaatg ttttcgattg catggataag aactgtcagg ccgaatgcgc
      241 ggatgagtga caggcctccc cttcatcttt cttgagcgcg aaccaccata cagcgctcgc
      301 ttttctcagt atttgcctca tccaattgct gaccgcccct gatggctcga ctgtccaagc
      361 ccacgccatc atcaatctcc acatggtcct ctcccacgag cagcgctcgg attttcggat
      421 agccgacccg tgccgatgac caacgccgtt cgtaaccttc tgctcacaca gcgcggtatc
      481 gtcttcgtat cctcgcacgg cccgcgtttg cgcgaaattc aacagcaggc gcaacagatc
      541 gtgcaggatg ctttccaaac ggccggggcg tgataacgcc acaaactcct cacttaccca
      601 tctcatttta tccgtatccg gatattcatc atcgccgatt acgaggcacg cggcagagat
      661 gtcgtgatgc aaggctcgag tgcaaggctc gattatcacg cgaggccctc gcggaactgc
      721 aagacagctc atgcatccat caacgcgatt ccatggttcc ccggacaacc ctcatggctc
      781 ctgacacggc ccaggcatcc aaaagaagtc aacgcttcgc accggtatta aactcaataa
      841 tttcccatag ttaagacaat ttccctggat ggagggttcc ttgaatgggt cctgtcccca
      901 tggcttttcg attcgcggta gccatcttgt ccccatcccg atccggtgca ttgtcgtttg
      961 aacaatgctt tccacctttg catcacctcc ccgttgtcca atggtttttc gtttcactcg
     1021 ccgccgggca tttgctcatc ggtcattcaa ttcggaaata actcttgggg aggaaaggat
     1081 attcgtgtcg gtgcgtatca gcatatacca cttatagtac gagggatatt tcgatgaaaa
     1141 aattgatctt ggtcggtttg atgggagtgc tggcgtcgac ggtttgggct tgttccgacg
     1201 atagcgacga tagcaaggat ggcagcggcg gagccaccaa cacgggaggc ggcagcaacg
     1261 tcggtggcgg ttccaacgaa ggcggcagcg gcggcagcaa cgnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nngcggttcc aacgaaggcg gcagcggcgg cagcaacgtc
     1441 ggtggcggtt ccaacgaggg cggtagcggc ggcagtaacg tcggtggcag ctctaacgaa
     1501 ggtggcagcg gcggcagcaa cgtcggtggc ggctccaacg aaggcggtag cggtggtgcc
     1561 agtaccgatg attgcgagag ctgccagatg gccgaatgcg ccagcgaagt caatgcttgc
     1621 ctagcgagtc agcaatgcca agactttgtg aaatgcaaca gcgcttgcac ggacaatgat
     1681 tgccggaacg cttgtttgaa tgagtatccg gatggagcag agctgtgggt taacggggta
     1741 caatgcacac ttgacaagtg taaggaacct tgcgggttca gtagttgacc gccgattcac
     1801 ctccttcagc tcatagagca ggcaaagcag cacgcgcttt ttcccgtcgt tcacctcatc
     1861 ccatccctat ccacccctga tggctcgagc gtgttcgggg tcacaccgtc acggttttgc
     1921 gcattgtcat ttcacgccag cgacgctccg acgttccgat agcttgacca accagatgta
     1981 ctcggtcccc gaccgaagcc gcgtcgattt cggtcgcgct ccggatggtt cggccatttt
     2041 tttgcgaagc ctcggaaatg gaaaagcgtt cgtgccgaac gatgcgtgaa aaaaaacacg
     2101 gaaaacccca aacgccaaag caatgaaacc actgctattc ttcgtcactt cctgctatct
     2161 tcgtaggccg gaggctgagc ccaggcttcc ttctactttg aaccagcctg ggcgatttcc
     2221 tcctatttca taaggatagg atgaccaaca ccgttcgtag cctccttctc acacggcgcg
     2281 gtatcgtatt cgtatcctcg cacggcccgc gcttacccga ttcccttctg caagccctcg
     2341 acctcgagct atcccaactc ggatatatcc tgtccagccg gattcgcgat cggctttccc
     2401 gttgcaacct cgaagaactc aacgagtttc gatcctggac catcaccacc ctggctgcat
     2461 ggctcggcgc cgatcaaaag cacaagccct tgtttcgccg atttccccgc ggtattccct
     2521 ccaataccgt cgagctgtgg tggaaaaaaa tccttgtcca ctacctgcaa gccgatggcc
     2581 aaacctgcct gttttgccgt cgaatcggca caacccacgt gctcgaccct tgcatgcatg
     2641 tcgtgtgtga ccattgtttc gacggctcga actacagcgc ctgtccgata tgcgaacacc
     2701 acgtcaaccg ggatagcccg ttttttcgcc cgacgcccga acgtcccctg ccctcggaaa
     2761 ccatcgtctt tcggctcatc gacctgggca acgatgaatc cgtcgaaacg aaaagccttt
     2821 tcgtgagtct ttgcgaacgc aagcaaccgc tttcccccgt ggatcgggat gcgctttttt
     2881 gtatcgtgaa agaacgtcct tcgcaggtgt tgtcatggct gccgcccacg atcgccgtgc
     2941 ggcaaaatat cgccatcgtg ttgggcacgc tcttttcggt gtgtgaatcg tcagaggtgc
     3001 tgccccacgc caaacgcttg gtcacgaccg ccaccgatgt gctgcgtttg ctcgcggtcc
     3061 tttccggtac cgatggatcg ttgatgcaag aaacgatctt tcgctctttc gccaaccccg
     3121 acgattccga tgagttatgc gtgcatacgg ctcgacgcct cgggatgtct ccgtcgtcgc
     3181 tcatcaagca aaaaatcaag gtaccggttc gcgtccatcg tttcaaagta gccaaactcc
     3241 cccgcccgtt acgtcgtgcg ctgctcggcc tgttggagtc gttacccgcc gattcccttg
     3301 tggaagatat gctgcgtcac cgcgcctatt gggtttgggt cggtgagttt ctccacccgc
     3361 atgagtatgc ttcgcgattc ccaaacgtgg cccgggcgtt tcagatcgtg cgcaaaaaag
     3421 cctccgacgg aacacctgcg ccgcggtttc gcacgtggcc ttcgagactc gaacaaaccc
     3481 tccgaaccgg ggatgcggat gcgatgcttt cggtgctttc ggagcgtccg ggcgagtatg
     3541 cgcggcgctt cgatttcgtg cttcggcaag cgagagacga gcgggcccag cggcgttatg
     3601 ccgaacaatt aattagtaaa atcaatgtat tatcgactcc ctggctcgtg gctttccgca
     3661 gttatcttcc cacgcgaatc gacaaagcgc cgattcgggt gttttggcca aagggtcgcg
     3721 gggcgtacgg cgtatcgcgt ccggatacgc gcacggctct tccacgtccc atcatcgagc
     3781 cagtcattca ggccatcgac aaggagcttc ttgcacggtt tggttcgaaa cgagcgtttg
     3841 cggcgggatg gatcgacgaa gggcttcgga cgatcatggt gccgatgcag gaagccacga
     3901 cgagtcgttc ggcggtatcg ttgcctcggg gctcacgggt gccgctggac aggggtcgtc
     3961 tgattcggtt gtttttgcat tggtgcgagc ccgcccacgg tgcgtggagt acggacctgg
     4021 atctatcggt cgggctctat gatgaatcat ggcgatacgt gggggtatgt tcgtattatg
     4081 aattgcaggc gaaagggccc gacggttcgg tgatcgcgca gagcgctggg gatttacggg
     4141 atgctccctg gcccgatggt gcgacggaat ttgtggatat tcatgctgat cgggcgcagg
     4201 cggcttcggt tcggtacgtg gtcatggtgg tgaaccgtta tggggggatg gcttgtagcc
     4261 agctcgagcg gtgttttgcg ggattgatgc ttcgggacga tccgatggga aagcatttcg
     4321 atccgcgaac cgtggcgttg aagttcgccc tgaatagcga gagcggggtt tttatgcctc
     4381 tcgtattgga tcttcgcgat cgggtgctcc actggctcga cgtgcaagtc gcgggtcagt
     4441 tggaaaggaa caacgtggag acttcgaata cggcgatcca gaagatttgt cccgagcaaa
     4501 tgacgtattt tgcgagcggc gttcgccctt gcttgttcga tctcggtttg cttcacgcgg
     4561 cggcacgatg caggcgcgta acccttcgcg gccggaatat cgtcgaattt ctgcgaaagc
     4621 ccgaagagag cgtgaacgac tttcatgcgc gtctggtgcg aggggaagga ggcgtgtgtc
     4681 gctcggcccc cgcggatgag caggagccaa tgtttgcgct cctttatcga ggagatgtcg
     4741 aattgccgga aggcagcgat gtctatgcgt tgtttcgcga gcagctcaca cccaatttgc
     4801 aggcaagcga tctattgtcg tgagggaaag ccgcgcctgg cactgagtct tcagcatccg
     4861 agcagtgctt ttgtatatta ttgaaaataa aggctatttc aggaaccccc gggttcgttt
     4921 gcacctgatg gggactcgat ggggtcagcg gcgggtgggg cagcgtcgtt gttatttgtg
     4981 ggttgtttcg atggggaggc tgcgatcggc cgtcgcaggt gccaaccttt gggccaatgg
     5041 atggagggag gcgcgatggt ttttctggtt ccttcgattt gaaggttcac ttcggagccg
     5101 agcaatacga gaaagctgct gagccatagc cagagcatga gcaaggcgac ggcggccagg
     5161 cttccgtaga agatggggta tcgcccgatg gtgcggacgt acgcggagaa tccccaagac
     5221 aaggcgaccc aggacaggcc ggtgatgatg gcgccgggcc agattcgttt ttggacatca
     5281 gcttcggggc tcatcgaagc gaatcggaaa aaggtggcgg tgcccaagat ggcgatgatg
     5341 gtggcggtgg gaagggcgcc atagcgcacg acttgcatcc atcctgcttc gacaaatcca
     5401 ccgcgaatga ttctatgcca cgtgggcgac gccagaacga gcagggtggt ggaaccggtg
     5461 gcgaccaaca ccagcatcaa gacgaaagcg agagccaacg agcgattgcg cagccaactg
     5521 cgtgcgtgtc ctgcttgtgc gatctggatg gcaacaatgg cggtatgaac gccaccggaa
     5581 gcgagccaaa gaaaaccgag caagctcaag ggagccaccg cttccgtgcc atctcccagc
     5641 cgatgcatct gttcgtcgac cagcccgaga gcaggctcag gggtcaccga aatcagcgat
     5701 tccaggacag aacgccggat ttcggtaccc gtgaagcgag tgaccgccca cccaaggatc
     5761 gcggctagcg gaatgagccc aagaaaaagc caaaacgcca tggcgctcgc ttgatgcacg
     5821 gcatcatgac gatcgagggc cagcacgatc gagacgatac gccgacgcaa tcggcgaaag
     5881 aaatcccaat atcgatcggg gatgggcact gtatccgttg tacacgtcct cgttgcctca
     5941 gggaaggggg cggtttcgac ttcgttttcc tcgtatcgcg cccatggtct acccccatcc
     6001 cacgaaggcc atgtcatcgc ggcctccaat tatacatata cggacatttg aatccttcct
     6061 ttccgctttc ataaaatgta ctaatactaa aggataatac acttatgcct caaatcgtta
     6121 ttacccatat atgtatcatt tatctggatg catcgatggc ataggtctac acgaatccgg
     6181 gaaggcgata tccgacaacg tcctaattac gttttccgcg agggcccacc catcgctggt
     6241 ttcgagcgag gcaaacgaca agcgctcatg gttgggcagg gcaagtcaaa gaagctgtgc
     6301 tacaagccgc gtgtcgaagg acgattcatg tacgcggacg ttcatactca tttaacccac
     6361 cgggcttttt cgggcgaaca ggacgatgtg gcggtgcgtg cggcccgggc tggtctcgat
     6421 tgcgtcatcg tcaacggcct ggaaccgcgt tccaatcgcg aagtgcttac cttatgtgct
     6481 cggcacccgc atttgtatcc agctcttggt atctatccgg tcgatgcggt ggcggcttcg
     6541 atcgatcgcg cctcgtggac gtacggattt gagcctcccg atgtattcga tgtggacgag
     6601 gaaatggact tcatcgattc ggtggccgat cagttggtcg cccttgggga atgcggtctg
     6661 gatcagtact gggtgcgtga tcaggctgcg gagcaggagc gcgttttgcg acgtttctgt
     6721 cagatagccc ttgctcatga caagcccctc attttgcatt cgcgaaaggc cgagcaacgc
     6781 acgctcgaca tcgtgctcga gatgggcatc acgcgcgctg actttcattg tttcggggga
     6841 aagctcaagc tggcgaaaca gatcgccgaa aaggggtttt atctatccat cccaccggtg
     6901 gtcatccgtt ctcaagcctt tcaacgcatc gtgcaagaag tgccgctcga tcggttgctc
     6961 acggaaacgg atgcccccta tatgggaccc gatgccgggg aaagaaacga gccttcgacg
     7021 attccccgag cggttgcggc catggcggaa ttggtacacc tgccagtcga acaggttcgg
     7081 gatatcctgc ggcagaactg tcgcgattta tttggtgtat gaaggcaaaa gggggtgagc
     7141 cggacggaat atttccccgc gagatggatt acttgcaggg attgagcagg gttccgcagg
     7201 tttttgcggg aggcggctgg gacggaggcg cggaatacac ggttttgaca agggtgagtt
     7261 cgcgtttcat atcctcgccg ggcgttacgg tgaaggaatc cttggcgggg ttgtaaccct
     7321 ttgtttttcc aagacaaata tggtttcccc ccgccagacg caccccgtct ttgaagtcag
     7381 cgaccttctt gttatcgcac caaatttctt cgcattccgg cgtacaagag aaggtgatca
     7441 aggcgtcttt gggtttttcc tcgggggcgg cctcgggtgc agaagcttct ttttccggct
     7501 ctgggggcgc agcttccggg acagtcgtcg ccacggttgg ggcggaagcc gaagccgagc
     7561 ttacggcagg agccggggta tccttgccgc tgaaaagata ccaggcgccg attcccagaa
     7621 gcacgacaaa agccatggcc gcgccgatca agaccggaat cagaagcttg ttcggcgatt
     7681 ttttgaattc gatctcgtcg gtatccggca tgaccgcggc ggcgggggca gcaggcggca
     7741 acggaggcat gggcgcaccc gtcacatcct gcgccgcggg tagcgactgc ggcgcatatt
     7801 gttgattttg tggatatccc tgcgcaggtt gcgcttgggc atcatagttg tggatcacag
     7861 gcgagggcgg ttccggcacc ggcgctgcgg gacccggcgc cgcaccgccg ctcggtctca
     7921 tcgtcggcac atgctgggaa gaacccacca attggaagag cgcgtggctc atctccccag
     7981 cgctcgagaa tcgttgcgaa ggactgaccg caagagcttt ggcaaaccag gagtcgagcg
     8041 taggcgatag gaccgccccc agctcacgag cgcgttggga agcgggaggc aaaggcgcgg
     8101 tcatctccgc ccacaggatg ttcaaatcag gcggcgtcgt ccggcaggcc aggaaaatcg
     8161 aacgccccgt cagcgcataa aacgctacca atccaagcgc atacacgtcc atcgtcggcg
     8221 aaggcgcggt ggaaggatcc gcttgttcgg cggacaacca tccgggcgtg gcggtccaac
     8281 cagggggagg cggcgaataa gccctcgccg ctcccattcc gaaatccgtg atccgaactt
     8341 gccaggaacg cgcatcgatc ggattggcaa aaatattttg cggcttgatg gcgcgatgca
     8401 cgattccatt tcgatgcgca aggtcaaggg actgagccac caatcgtaag atggcgtcga
     8461 gttccgcaag tcccatcggc ccctgctccg acacgatcgt atgcaacgat gggaacgtta
     8521 catactcgcc cagcacataa ggcgcattca atgtcggttc tgtctggaat tcataagcct
     8581 tggcaatggc atcggattca agcgccgcgg cccgttgcgc gtcaccacag agtgcggccc
     8641 acgcatccgg actttgcgta atctctgggc gtaatagctt cagagcatac gcatagcccg
     8701 tcgtcatatc gcgcacgtcg tagacttcac cgacctcgcc ggcaccgaga agccgcgcca
     8761 cctg
//
LOCUS       DAOSOR010000197         8753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8002_length_8753_cov_4.455967, whole genome shotgun sequence.
ACCESSION   DAOSOR010000197 DAOSOR010000000
VERSION     DAOSOR010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8753
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8002_length_8753_cov_4.455967"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PK710_12010"
     CDS             <1..227
                     /locus_tag="PK710_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10491.1"
                     /translation="LYVYLDKDKDVVECSLLAASSVLPAEELDSANLNWGEAQIPNGK
                     DDSRWVTIEVRHVSGPCSEQYSWEVTAKGN"
     gene            complement(280..1614)
                     /locus_tag="PK710_12015"
     CDS             complement(280..1614)
                     /locus_tag="PK710_12015"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT10492.1"
                     /translation="MSARNQDDVESVPVYVIESIPVHGDHRVSLEEREREAIPTPAHL
                     RLGPELGHGAMGHVHSALDRNLLRHVALKRLDKHHVSVDMYRDGFIAEAQITGQLEHP
                     NIVPVHELAVGDNGVPYFTMKLVRGADFKQWLANPERPPGSVARLEQGLEIIIKICDA
                     VAYAHHRGVIHRDLKPENIMVGDFGQVYLMDFGLARLMKTKPASGARAQMEAHGPVGT
                     VTYMAPEQALGDPDEMDERSDVFGLGALLYELVSGQTPYGRPRNMNDAFFRATGGKVI
                     PLKTACAGVPVPLRLEEIVMKAVAPAKSDRYQSVVELQKDLRDLLRGGIHLPTETFAP
                     GSMIIREGDRGDKAYMITQGTCCAYRVVDGQRETLTTMSVGEVFGEMALLLDEPRAAS
                     VEAVDRVTVLVLDKKTMNEGIGVNAWTSALVRAMAHRFRNLEQQVRDSGMRR"
     gene            complement(1673..2254)
                     /locus_tag="PK710_12020"
     CDS             complement(1673..2254)
                     /locus_tag="PK710_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOT10493.1"
                     /translation="MTTAPCKTWILAAAMLLSACTPKPTTETMNTNASSQQSLQLSSS
                     AFEPASSIPTRYTCEGEDLSPPLSWKGIPEGTQSLVLIVDDPDAPDPAAPKMTWVHWI
                     LYDIPPSCESLAEGIRQLPAGTKQGINSWKKTGYGGPCPPIGNHRYFHKLYALDKTLA
                     SMETPDKDAVVAAMQGHILAQAVLMGTYQKKGK"
     gene            complement(2432..3364)
                     /locus_tag="PK710_12025"
     CDS             complement(2432..3364)
                     /locus_tag="PK710_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10494.1"
                     /translation="MKASRDNGTSVKTSRASRWLDGWQAGLIVVAIGVLVVSIVIPRP
                     RLPQEIPSPRPNWAKLGEVARQDSRLATLAEQEPLPFEVRQVGESFRSFGLAVAAGDA
                     ASTQRIQLLLQQQIAAVREPDLLLRLRAWQTQDFLKEVAAFETTGNESQGLRELGGEI
                     VRIAQQAGWLVPRGSGYHWLIDDFTRQVLFRKRWNEILHLQRAPFLLSIDEERVFYAF
                     LFRFPGHHSWHPDPLRRCQSANEQLLKKVTAFGAIDPSYPTDYARGVLLLRLDRPQLA
                     VEPLAQFLDNNPDGPYTLHARNALRFAQNQLLPP"
     gene            3410..3724
                     /locus_tag="PK710_12030"
     CDS             3410..3724
                     /locus_tag="PK710_12030"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOT10495.1"
                     /translation="MIEKRKYPRQKVELAATLSEEGTGEAVEGRVQDLSIGGMFFLCH
                     SKLPFGTKVKISVDFGPSLGTQQLPAVVRWTTPQGVGLQFGLLGARETHAIAQTLQKK
                     KA"
     gene            complement(4030..5127)
                     /locus_tag="PK710_12035"
     CDS             complement(4030..5127)
                     /locus_tag="PK710_12035"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOT10496.1"
                     /translation="MNQPLDARWLKKVAQKIAETSPEFDWDRVETAVFQRLEREPAPR
                     APSSPAAYGRAVTAGLIAAAVAALTLSVSQTPRHDLSTALAGPTVHDAVPADIVATHE
                     PQAFEHEQWARFHLHAPGRLRVLRMDDRVSLHLLEGKLEVDVSRRDALEAVTIRAGNV
                     RVAVHGTLFSVELREGVVQTQVQQGSVVVGPASRGNTEGWLLIGPSTGYFDATIGQRV
                     AGHSYVDQPLVLHRSETPSSPPVIVQPTQPQRDLSAPSPVASPKIRPPQPLPNALDDH
                     LAATTLQAIRQNITACYRRHLPSSNEGVTIHAQIHISIHVAPDGHVTFARFDPPLSPR
                     AQSCASEAVQNAAFPSARESSVLELSMQLSP"
     gene            complement(5124..5852)
                     /locus_tag="PK710_12040"
     CDS             complement(5124..5852)
                     /locus_tag="PK710_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT10497.1"
                     /translation="MNSPSPTLAAFPVVGYLQPTDLLICQFRFLIRTIKTVAAFAPKV
                     DPLISRCQLGDKTAFRELFHRHRGDVGRIVQRMLGPVPEVEDIIQDVFIQVYRTLQDF
                     QGNSKFSTWLYRVAVNTVLMHRRAQQSRPVLVAESTRPVPNDRLEPDEEAARRERIRA
                     FYAVLDRLPEKKRTVFILHEIEGMNPKEIASLVHAPALTVRTRLFYARRDILRMMQQE
                     PALASLASELLGSDDDQPSSEEES"
     gene            6387..8477
                     /gene="fusA"
                     /locus_tag="PK710_12045"
     CDS             6387..8477
                     /gene="fusA"
                     /locus_tag="PK710_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOT10498.1"
                     /translation="MIRDQRFIRNIGISAHIDSGKTTLTERILFYTQRIHAIHEVKGK
                     DSVGATMDSMDLERERGITIQSAATHCFWQNHAINIIDTPGHVDFTIEVERALRILDG
                     AVLVLCAVAGVQSQSVTVDRQMRRYGIPRVAFINKCDRSGANPLRVANQLRDKLGLNP
                     VLLQLPIGLESHFEGVIDLVHEQAIYFDGPQGQTVRNEPIAEAFRAEALRAREAMLDA
                     VSMFSDELTEAMLEDRVTVELLNQAIRKATIAQQITPVFVGSAYKNKGVQALLDGITS
                     YLPAPGDRVHHANSVDDGHEVPLTGSAQDPLVLMAFKVDDGRHGQLTYLRIFQGTLKR
                     SSTILNMRTQKKHRVGRLVRMHAEEMEDIDHAIAGDIVAMFGIDCASSDTFTDGTVSL
                     SLASMHVPTPVISLTVKTDTKNLAGLSKALRRFGKEDPTFRVASDPETGETVISGMGE
                     LHLEVYLERMRREYSVPVEVSPPRVAYRETITAAAVFDYTHKKQTGGAGQYGRVGGRI
                     EVNTEEEFTFVDATVGGVIPREFISSCEKGFRSCLNKGPLIGAPLVNVRVIVNDGAAH
                     AVDSSDVAFQEAARGAFRQVIGRCKPQILEPVMKVVVETPAEFQGNVVGTLLKRRGII
                     VGTTEENGFVQIEAEVPLAEVFGYSTVLRSSSQGKGEFSMEFSRYCPVPASTAEDLIR
                     SYQERQRNTKDGAS"
     gene            8474..>8753
                     /locus_tag="PK710_12050"
     CDS             8474..>8753
                     /locus_tag="PK710_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10499.1"
                     /translation="MITQEVIERSPIRALEQAISGGLEPQQLGVIVARAGVGKTACLV
                     QIGLDALLCGKTVLHVSNESPVVHLRSYYDELFRGIEQGTGLEDSPTVL"
BASE COUNT         1879 a         2144 c         2836 g         1894 t
ORIGIN      
        1 atttgtacgt ctacctcgac aaggataaag acgtcgtgga gtgttccctg ctcgctgctt
       61 catcggttct tcccgcggag gaattggatt cggccaactt gaattgggga gaagcccaaa
      121 tccccaacgg caaagacgac agtcggtggg tcaccattga ggtacggcac gtgtcgggcc
      181 cctgtagcga gcagtattct tgggaagtga cggcgaaagg caattgacgc acgccaccga
      241 tcgccttgga agcataacta accggattta cgcggaatat tagcgtcgca tgccggaatc
      301 acgcacttgt tgttcgagat tgcgaaaacg atgggccatg gctcgcacca aggccgaggt
      361 ccaggcattg acgccgattc cttcattcat ggtttttttg tcgagtacga gaacggtgac
      421 ccggtcgacg gcttccacgg aggctgccct cggttcatcg agcaacaggg ccatttcccc
      481 aaacacttcc cccacgctca tggtcgtcag tgtttctcgc tggccgtcga cgacgcggta
      541 ggcgcagcaa gtgccttggg taatcatgta ggctttgtcg ccgcgatctc cttcccgaat
      601 gatcatactg cctggggcga acgtttccgt gggcaggtgg attccgcctc tcagcaggtc
      661 tcggaggtct ttttggagtt cgacgacgct ttgatagcgg tcggatttgg cgggggcgac
      721 tgctttcatg acgatttctt cgagtcgcag gggaacgggg actccggcgc aggcggtttt
      781 taagggaatg acctttccgc ccgtggcgcg aaagaaggca tcgttcatat ttcgggggcg
      841 tccgtagggg gtttggccac ttaccaactc atacagcagg gcgccgaggc cgaagacatc
      901 ggaacgttca tccatttcat ccgggtcacc gagggcttgt tctggagcca tgtaggtaac
      961 cgttcccacc ggaccgtggg cctccatttg ggcacgcgcc ccggaggcgg gtttggtttt
     1021 catcaggcgg gcgaggccaa agtccatgag gtaaacctgt ccgaaatcac ccaccatgat
     1081 gttttctggt ttcaagtcgc gatggatgac gccccgatgg tgcgcatagg ccaccgcatc
     1141 acaaattttt ataatgattt caagaccttg ttcaagacgc gccacggaac cgggcggcct
     1201 ttcgggattg gcgagccatt gtttgaaatc cgcgccgcgc acgagtttca tggtgaagta
     1261 cggcacccca ttgtcaccca cggccagctc atgcacgggg acgatattcg gatgttcgag
     1321 ttgcccagtg atttgtgctt ccgcgatgaa accgtcgcgg tacatatcca cggaaacatg
     1381 atgtttgtcg agtcgtttga gggcgacgtg gcgcagcaag ttacggtcga gggcggagtg
     1441 gacatggccc atggcaccgt gtcccaattc ggggccgaga cgcagatggg cgggtgttgg
     1501 gatggcctct cgctctcgct cctctaggga cacgcggtgg tcgccgtgaa cgggaatcga
     1561 ttcgatcaca taaaccggaa cgctttcgac atcgtcttgg ttacgggcgc tcatggcggg
     1621 ttctcctgac tacgtcgcgg cttgccaccg caagcccgaa cgcaccaacg ggttatttac
     1681 cctttttttg gtaagtgccc atcaaaaccg cttgcgcgag gatatgacct tgcatcgcgg
     1741 ccacgacagc gtccttgtct ggtgtttcca tcgaggccag ggttttatcg agagcgtaga
     1801 gtttatgaaa gtagcggtga ttgccgatag gggggcaagg tccgccgtat cccgtttttt
     1861 tccagctatt gattccttgt ttggttcccg cgggcagttg gcgaattcct tcggccaagc
     1921 tttcgcaaga aggggggata tcgtaaagaa tccagtgaac ccaagtcatt ttcggagccg
     1981 cggggtccgg agcatcgggg tcatcgacga tcaacaccag gctttgggtg ccttccggga
     2041 tgcctttcca cgacaaaggg ggcgaaagat cctcgccctc gcaggtatat cgagtcggaa
     2101 tcgacgatgc aggctcgaaa gcggaagagg atagttgaag ggattgctgg gaagaagcgt
     2161 tggtgttcat ggtttcggtg gtgggtttgg gagtacaggc agacaagagc atggctgccg
     2221 cgaggatcca agttttgcat ggggcggttg tcatcgattt tttccttggc cttggcgaat
     2281 gggttagccc tgggtattcg agacgaatgc caccggatgg tgcgcgttgc ggcatggcaa
     2341 acggcagtag tataagggat ccgcgccacg ggggggcagg gaatggaaaa caagggcaat
     2401 ggccggccgc gcgtcggaaa cgcatgcgtc attacggagg caatagctga ttttgtgcaa
     2461 accgaagcgc gttgcgtgca tgtagcgtat agggaccatc gggattgtta tcgagaaatt
     2521 gagccagcgg ttcgaccgcg agttgcgggc ggtccaggcg aagaagcagc acgccacgag
     2581 cataatccgt gggataagat ggatcgatgg cgccaaaagc cgtcactttt ttcagcaatt
     2641 gttcattggc cgattggcat cggcgcagcg gatcgggatg ccaggaatgg tggccgggaa
     2701 agcggaacag aaacgcgtaa aagacccgct cttcatcgat ggacaacaag aagggagcgc
     2761 gctgcagatg caggatttcg ttccatcgtt ttcggaacag gacctggcga gtgaaatcat
     2821 cgatcaacca gtggtagcca gagccgcgcg gcacgagcca cccggcttgt tgcgcgatac
     2881 gcacgatctc tccccccaac tcccgaagcc cctgggattc atttcccgtc gtttcgaagg
     2941 cagctacttc tttcaaaaag tcctgcgttt gccaggcacg cagacgcaag agcaggtcag
     3001 gctcccgcac ggccgcaatt tgctgctgca gcagcagttg tatcctttgg gtactcgcgg
     3061 catcgcccgc tgcgaccgct agtccgaaag atcgaaacga ctcaccaact tgtcggactt
     3121 cgaaaggcag cggctcttgc tcggccaagg tagcaagtcg gctgtcttgc cgagccacct
     3181 cacccagctt ggcccaattg ggtcggggag aagggatctc ctgggggagt cgtgggcgag
     3241 gaatgacaat ggaaacgaca agcactccga tggcaacgac gatgagaccg gcttgccatc
     3301 catcgagcca acgagaagca cgagacgtct tgacagaagt gccattgtct cgcgaagctt
     3361 tcatggcctt cggacccatg ttccgaggat ggaaagagca ggacgacccg tgatagaaaa
     3421 gcgcaagtat ccccgccaga aagtggaact agcagcgacc ttgtcggagg aagggaccgg
     3481 tgaggccgtc gagggccggg tacaggattt gagcattggt ggcatgttct ttctatgtca
     3541 cagcaaactc ccgttcggaa caaaagtgaa gatctcggtg gactttggtc catcgctagg
     3601 gacacagcaa cttccggcgg tggtccgatg gacaacgcca cagggggtgg gcttgcagtt
     3661 cgggctattg ggtgcgcgag agacgcatgc cattgctcaa acactccaaa aaaagaaggc
     3721 atagggtaat gattgggcca atagggatgc tgcaagcgcg atatccatgc catggccggt
     3781 tttccgatcc tgtcgtgaaa acgctttccg tgcggtgaaa acggattttt ttggcacccc
     3841 atcgctcggt gggattcacg cacggaaatg ggctatgcgc gaccggttca tggctcgtgt
     3901 cgtatcgccg ctcgcgcatt tttcccttta ttaacgaata gttgaatagg attcgtcgat
     3961 tccgagagtt gcgatacggc gcaggcaagc agagattccc tatttcctgc tcgccgggga
     4021 gctgtcgtct catggggaga gctgcatcga tagttcgagc acggatgatt ccctggcgct
     4081 agggaacgcg gcgttttgca cggcctcgct cgcgcaggat tgggctcgtg gtgataacgg
     4141 aggatcaaaa cgggcgaaag tgacatgacc atcgggagcc acatgaatcg agatatggat
     4201 ctgggcgtga atggtgacgc cttcattgga gctgggcagg tgacggcggt agcaagcggt
     4261 gatgttctga cgaatggcct gcagggtggt ggcggcgagg tggtcatcga gagcgttggg
     4321 cagtggttgt ggcgggcgaa ttttcgggga agccacgggg gaaggggcgg acaggtcgcg
     4381 ctgcggctgg gttggttgga ctatgaccgg aggggaggat ggggtttccg aacggtggag
     4441 cacgagcggt tgatccacat aggaatggcc tgccacacgc tgtccgatgg tggcgtcgaa
     4501 atagccggtg gaaggcccga tgagaagcca tccttccgta ttgccgcgcg aggccggtcc
     4561 cacgaccacg gagccttgct gcacctgcgt ttgcaccacg ccctcgcgga gttcgacgga
     4621 aaacagcgta ccatgaaccg caacccgcac attgccagcg cgaatggtga cggcttccag
     4681 ggcatcacga cgggacacgt cgacctcgag ctttccttcg agtaagtgaa gcgatacgcg
     4741 gtcatccatg cgcaaaaccc gtagacgtcc tggggcgtgc aaatggaaac gagcccattg
     4801 ctcgtgctcg aaagcctggg gttcgtgggt agcgacgatg tcagcaggca cggcatcgtg
     4861 aaccgttgga cctgccaagg cagtggacag gtcatgacgg ggggtttgag aaacggaaag
     4921 ggtgagggcg gcaacggccg cggcgatcaa gcctgcggtt acggcgcgtc cataggccgc
     4981 gggagacgac ggggcgcggg gggcgggctc tcgttccaat cgctgaaaaa cagcggtttc
     5041 tactcgatcc caatcgaatt ccggtgatgt ctcggctatc ttttgcgcga cctttttcaa
     5101 ccatcgggca tcgaggggct ggttcatgac tcctcctcgg aagatggctg gtcgtcgtca
     5161 gagcccagca actcgctcgc cagcgaggca agggcgggct cctgctgcat catgcgcaaa
     5221 atatcgcgtc gggcgtaaaa aagtcttgtt cgcacggtca gcgctggagc gtgtacgagc
     5281 gaggcgattt ctttcgggtt cattccctcg atttcgtgca gaatgaacac ggttcgtttt
     5341 ttttcgggca agcggtccag cacggcataa aaagcacgga tgcgttcgcg gcgggcggcc
     5401 tcctcatcgg gctcgagcct atcgttaggc accgggcgag tggattcagc gacgagaaca
     5461 gggcgtgatt gctgggctcg gcgatgcatg agcacggtat tgacggcgac gcgatacagc
     5521 caagtcgaga acttagaatt gccctgaaag tcctgaaggg tacgataaac ttggataaat
     5581 acgtcttgaa tgatgtcttc cacttccgga accgggccga gcattcgttg cacgatgcgt
     5641 cccacatccc cacgatgtcg atgaaacagc tcgcggaacg cggttttatc gcccaattgg
     5701 caacgggaga tcagggggtc cactttggga gcgaacgcgg ccacggtttt gattgtacga
     5761 attaaaaaac ggaattggca gataagaagg tctgtaggtt ggagatagcc aacgacggga
     5821 aaggcggcaa gggtgggaga gggggaattc accgggctcg tttcatgaga tgctcgcgat
     5881 ttttggattg ttcatggggt atggacgaca aggatttggg tggaaggtcg cggcctcggt
     5941 tggcggtgat aacgtgaaag aacccacgga agatgggatt tccaatctta ttgaaacagt
     6001 gattacgctt gtggttggag agggcaggcg tgtgagtaag caagaggagg gggagtcacg
     6061 ggcggggatg ccaccgtagg gggcggattt ggggttaaaa tccgtttgcg gtggaggatc
     6121 agggcaagac acgtcgggga cggattttca tcacgtccat cacatccgca gcgatcaggc
     6181 ccaccaccaa ggtcaatgcg agaacgagca tgcgggcaac catgcgggtg tgggttggaa
     6241 ggggccaagt attcgacaaa actattttat atattttgcg ggagatcctg ttccgaccga
     6301 gcttgcgcga taaactcgtg tccgctagat gcccgcggtc gcgaaggcgt tctgcttgcc
     6361 gtgactttcc cccacgtggt gctgttgtga tacgagatca acgattcatt cgaaacattg
     6421 ggattagtgc tcatatcgac tcgggcaaga cgacgctcac ggagcggatc ttattttaca
     6481 cgcagcggat ccatgcgatt catgaggtaa aaggcaaaga cagtgtggga gcgacgatgg
     6541 actcgatgga tctcgagcga gagcgtggga tcacgattca gtctgccgcg actcattgtt
     6601 tttggcaaaa ccatgcgatc aacatcatcg acactccagg gcatgtggac ttcaccatag
     6661 aggtggagcg agcgcttcgc attttggacg gtgcggtatt ggttttgtgt gcggtggcgg
     6721 gggttcaatc gcaatcggtc accgtggatc ggcagatgcg tcgatatggg attccacggg
     6781 tcgcattcat caacaagtgt gatcgttctg gggcaaaccc gcttcgtgtg gcgaatcaat
     6841 tgcgggacaa gcttgggctc aacccggtat tgcttcagtt gcccatcggt ttggaaagcc
     6901 acttcgaagg tgtcatcgat ctcgttcatg agcaggcgat atatttcgat ggtcctcagg
     6961 gacagacggt acgcaacgag cccattgccg aggcgtttcg ggccgaagcc ttgcgcgcca
     7021 gagaagcgat gctcgatgcg gtatcgatgt tttccgacga gttgacggag gcgatgctcg
     7081 aggacagggt gacggtcgaa ttattgaatc aggcgatccg caaggcaacg atagcgcaac
     7141 agatcacgcc ggtattcgtt ggatcagcct acaaaaacaa gggagtgcag gcacttctcg
     7201 acggtatcac gagttatctt cccgctcctg gggatcgggt tcatcatgcc aattcggtcg
     7261 acgatgggca tgaggttccg ttgaccggct ccgcacaaga tccgctggta ttgatggcat
     7321 tcaaggtgga cgacggtcgt catggtcaac tcacctactt gcgtattttc caaggcacgc
     7381 tgaaacggtc ttcgaccatt ttgaacatgc ggacgcagaa aaagcatcgt gtggggcgtc
     7441 tggtgcggat gcatgccgag gagatggagg acatcgacca tgcgattgct ggggatatcg
     7501 ttgcgatgtt cgggatcgac tgtgcgtcct cggatacgtt cacggacggg acggtatcgt
     7561 tatcgttggc gtcgatgcat gtgccgacgc ctgtgatttc gttgacggtc aagacggaca
     7621 ccaaaaattt ggcggggtta tcgaaggcat tacgaaggtt tgggaaagag gatccgacat
     7681 tccgggtggc atcggatccg gagacgggag agacggtcat ttcggggatg ggggagttac
     7741 atctcgaagt atacttggag aggatgcgaa gggagtattc ggtaccggtg gaggtgagtc
     7801 cgccacgggt agcgtatcgg gagacgatca cggcggcagc ggtatttgac tacacgcata
     7861 aaaaacagac aggtggagcg ggtcaatacg gtcgggtggg aggacggatc gaagtgaaca
     7921 cggaggaaga atttacgttt gtggacgcga cggtgggggg agtcataccg cgggagttca
     7981 tctcttcgtg tgagaagggg tttcggtctt gcttgaacaa ggggcctttg attggcgcgc
     8041 ctttggtgaa tgtgcgtgtg atcgtaaacg atggtgcggc ccacgcggtt gattcgtcgg
     8101 atgtggcgtt tcaggaggcg gcgcgggggg catttcgcca ggtcattggg cgctgcaagc
     8161 cccaaatatt ggagccggtg atgaaggtgg tggtggagac gcccgcggaa ttccagggga
     8221 atgtggtggg caccctgctc aaacgacgtg gcattatcgt gggcacgacg gaagaaaacg
     8281 ggttcgttca gatcgaggcg gaggtaccct tggcggaggt gtttgggtat tcgacggtgt
     8341 tacggtcgtc gtcccaggga aaaggcgagt tttccatgga gttttcccgc tactgccccg
     8401 ttcccgcgag tactgccgag gacctgattc gcagctatca agagcgtcaa cgaaacacaa
     8461 aggacggtgc atcgtgatta cgcaggaagt cattgaacgc agtcccattc gagctttgga
     8521 gcaggcgata agcggtggtt tggagcctca gcaacttggc gtcatcgttg cgcgcgctgg
     8581 agtcggcaag accgcttgtc tggtgcagat cggtttggac gcgctgttgt gtggcaaaac
     8641 ggtgctgcac gtgtcgaatg aatcacccgt ggtgcatctt cgctcgtatt atgatgagct
     8701 gtttcgtggg atcgagcagg ggacggggct tgaggattct cccacggtat tgc
//
LOCUS       DAOSOR010000198         8730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8032_length_8730_cov_4.422363, whole genome shotgun sequence.
ACCESSION   DAOSOR010000198 DAOSOR010000000
VERSION     DAOSOR010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8730
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8032_length_8730_cov_4.422363"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="PK710_12055"
     CDS             complement(<1..1024)
                     /locus_tag="PK710_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOT10500.1"
                     /translation="MSVFPANIHRRILVVDDEPALRDTLSILFRRESYDVVTAPGCRS
                     AIEAIQQADPPFPVVLTDLRMPDGDGFEVLEAAKARSEATEIIVMTAFHEHAFDAMRR
                     GAYDFVSKPFPSLREVLERVGKAAEKAGIVAENVALRAQVEAAGDKSWVARSPGMLRV
                     MEIIEKVADTKTTVLITGESGTGKERVARLIHEKGGRAHKPFLVVNCGALPESLMESE
                     LFGHERGAFTGASSKHLGMLRAADGGTLLLDEVGELPTSLQVKLLRVLQEKKVRPVGG
                     TTESEVDVRILAATNADIETAVSEGRFRKDLYYRLNVIRLVLPPLRERPEDLERLVDS
                     FLRRFSA"
     gene            complement(1118..3019)
                     /locus_tag="PK710_12060"
     CDS             complement(1118..3019)
                     /locus_tag="PK710_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10501.1"
                     /translation="MDDKQSRLAEQRRVWYAVIVAWLAALVAVNLVAYVPTHDGPQHI
                     YLGHLLNHFSDPGSIYPLYFRPARPLAALGFSSIFCPLDDVLGWQIAFRLSLSVIVSM
                     WGWSVWALARTIDPRRAIVGVMGFATAFQWTLYMGFFSWMISMALGLGTLAVALRLPW
                     SVQRRVLIALLLAIHAVAHPFPALVTSLVLVTLVLFHHATRRWWRELIWLALMGIPVV
                     LVIAYANGWFGSETVSLPAGEPSPERSFAMVMRDALNLFVGGPYWRSIPVSLATAFAV
                     GWGAWRWKTRRSGATDRALWLVGSLLLLIFFWAPLHLGTWEFFSPRFLAPASLLLWVA
                     LPLETLSRTAYLVVFSLVCVHGGAALAWSTRFHRELFNRSADILALAREPIRRHGPRL
                     PMIVDTRLGYGNEMDRWYPYFEPSLNVGTLFAIEQGGVVPYTFTSVPQLHGLVFSEEG
                     KSRMPAAPYRQVYGPVYRAAVAQSNQHVASAAMVSWASFGSSFEDVVYIGPRHELETL
                     VSRGYRVEKHRGDGAILRFEGCPTDVAVMVSGPLPHALTVEYGWLPVTLHSYSMTAPT
                     GTQPIENKINFSFSGMPCGPVWVRAWLDRDDTGTLTSADRVCNGTDREGRMHFEITHE
                     TRHVVCSLD"
     gene            3098..4675
                     /locus_tag="PK710_12065"
     CDS             3098..4675
                     /locus_tag="PK710_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10502.1"
                     /translation="MSFLSSPCRAYWLAVVLLGCGDAASSPSPTPEPSTLDASTTAAS
                     ISLEPSSTITMAPGQTTEITVIVEPPDVYVVRFALLGESLDASLDVDKRYTSANGHTS
                     VKLVASESSTTFRLRASAGPTAVAEVPVSVSNEGFASVLVLPSYKGIRSTPQWTASAL
                     AGWTCAQIPGQPPDDGPIVGVGSSKGIRLESLPVGPTIAVTLRSQQAVGGCTEVTNLA
                     PGESREVSVKVSDMPIRSDDTRLDITMASDPLEAIPLLDELTDSFLQRLFPSKNSEAK
                     TLLDAMQSELPASVDFDQARLFGGWDALVAAWLDKSGQSLREHIQAWVDDGLLPLRQG
                     EWLTGRLTTIAGSNTHGVLNLERFHGLAHERTGIPTEIVVSVGFEPGDIMMVGGKISW
                     LPSRFVGAAADVGAKNIVGTADAIDALVALIGCNQLPALLSSTYPLSPSCDAQCLESA
                     CHDAVSSLWNGACHSSAISLDTVQLSFTISGTATVNGDAAVIGLDAAWVGTQSHGDEE
                     MLVRGNVRGSLPSTQSR"
     gene            4684..6654
                     /locus_tag="PK710_12070"
     CDS             4684..6654
                     /locus_tag="PK710_12070"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOT10503.1"
                     /translation="MKLPLEWIVTALFSIGTLTVLAFLFRTPSIPTSPHLEKHHGETS
                     AIAAPPIDSIPSLGPSFDEDASLPAHAVSIASYTLQANLDTRSHVIEGKGTIHWVNRS
                     STSITELPIHLYANAFRNEASRFLSDRRPGGRGTQRIRDFGFIDVLSFVVSSHGNDNL
                     WPKAIRADSAFPSDDTDMLVPLERPVAAGESIDIEVAWRTKLPSIVERMGYHDTFHMA
                     AQWYPKIAKHESDGRFAHFSFDRLSEFYADFGTYDITISVPSTFLVGATGPCVSDIIE
                     GDRRIVRHVQADIHDFAFAAWDGFREVRRQVDGIDVRLLFPPGYERVATEQLDVTTFG
                     LRYYGKRFGSYPYAMLTIVHPPSGAGEAGGMEYPTLFTTGGSWWERGPNGMARSVTLH
                     ELAHQYFQGMVATNEATWPMLDEGITSFVELDALDTGWPDAGAFSLSDYGVSAATMAR
                     WFGLTAGTDDIIAKPAAEFSSGHAYARLVYWRSALLWRTLDRVFDGTIMQALGRYARR
                     YRFTHPEPEHLLSAVRDVAGKQAEDALRISLFERGWIDVAINDAFCGSLSCQAIVSRR
                     GNLELPVDVLWIHTDGSRVMTRWEGKEVQTTLHKEGLIPVRHIVLDPEHKILLDDDLS
                     NNAIFLGQRPRAWRTRSYISYLAILGLQAVMP"
     gene            6651..7523
                     /locus_tag="PK710_12075"
     CDS             6651..7523
                     /locus_tag="PK710_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10504.1"
                     /translation="MIAVYRLRWLPAVPYAMAGYAIRLALAFVLIHPIASVVERTLAG
                     WPANEHMMFHSGSLLFSEVLRLHGVAFHGLLEQLALGALLMIPIGIVIHAFVIASFVD
                     PDTRDLKAIASLAIARMLPVAVVSLLSLLAISGMMILSWVIREGVRSMLPFDTRTNDM
                     LSLGSILVGALAVVVSLVMTDLARIATIRDKLGVAEAIALALHSVRKRPFEIFGAFGA
                     RVSASIFLAMVGLTFPLMFGIETSIPASCWIIVQQVIVVVMSCLRLSWLARAASFQPR
                     LSQQGSIDLEIPMR"
     gene            complement(7479..>8730)
                     /locus_tag="PK710_12080"
     CDS             complement(7479..>8730)
                     /locus_tag="PK710_12080"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOT10505.1"
                     /translation="TDSFEVTVFDGRLPSGVDITLLEVPGCFDREGIYGAGDEDYPDN
                     DMRFALFSRAVVALLLSRMRAGSPFGIVHLHDWPTSLVPLFGKRLGNQDLEECTKFVL
                     TLHDLTHQGLSDASKLDQLGIGGDLFHPDGVEFYGKINILKAGILFSDAMTTVSETYA
                     REILKSPHGRGLEGVLSAHKNKLTGIINGIDYSIWNPTIDPHLPSRFSGQDPSQKTQC
                     KGRLLQELGLALRAQRPLLAFAGTLHEQNGIDLLLDALPEIMRCDVGLVVAGHGTGAI
                     NARLEEAARKWKDDFVFLSSPSDPMLHRIFGGADIVLVPSRFEPCGVYQRYAHRYGAV
                     PVVHATGGLIDTVVDCDATLETGSGFVFDEPTAQSLVSAVQRAVASYALPGWARLRKR
                     VMNLDLGWDRSAHRYLEIYRSLLR"
BASE COUNT         1885 a         2624 c         2319 g         1902 t
ORIGIN      
        1 cggcggaaaa acgtcggaga aatgaatcca ccagacgctc caggtcctcc ggacgctccc
       61 gcagcggggg caaaaccagg cgaatcacgt tgagtcgata gtataggtct tttcgaaatc
      121 gaccttccga taccgccgtc tcgatatccg cattggttgc cgccaggatt cggacatcga
      181 cttccgattc cgtggttccc cccacgggtc gcactttttt ctcttgtaat acccgcagca
      241 gcttcacttg aagagaggta ggcaactctc ctacttcatc caataatagc gttcccccat
      301 ccgccgcccg cagcatcccc agatgtttgg aggaggctcc tgtgaaagcg ccccgctcat
      361 gaccgaaaag ctcgctttcc atcaacgatt ccggcaaagc cccgcagttg actaccaaaa
      421 atggcttgtg ggcgcgccct cctttttcgt ggatcaaacg cgccacccgc tccttgcccg
      481 tcccgctctc ccccgtaatc aacaccgtgg tcttcgtatc cgctaccttc tcgattatct
      541 ccatgactcg aagcatcccc ggacttcgag ccacccagga cttgtccccc gccgcttcca
      601 cctgcgcgcg caatgctacg ttttccgcga caattcccgc tttttccgcg gctttgccca
      661 ccctctcgag cacttctcga aggctgggaa atggcttcga cacgaaatcg tatgcaccac
      721 gacgcatcgc atcgaaagca tgctcgtgaa acgcggtcat cacgatgatt tccgtcgctt
      781 cgctccgcgc tttcgccgct tccagcactt cgaacccatc gccatcaggc atcctcaggt
      841 ccgtcaacac caccggaaac ggaggatcgg cttgctgaat cgcctcgatc gctgaacgac
      901 accccggcgc ggtcacgaca tcgtaggatt cccgacgaaa taatatgctc agcgtgtctc
      961 gcagggcggg ctcatcgtcg accaccaaga tccgtcgatg aatgttagcc gggaatacgc
     1021 tcattcacac catcttactc gcggaatccg cgaaggtcac gcttcgttca cccattacct
     1081 ggttttccct ttgcaaagcg ccgtctgcat caccaactca atccaagctg cagacaacgt
     1141 gtctggtctc gtgagtgatt tcgaagtgca ttcgaccctc acggtccgtc ccgttacaaa
     1201 ctcgatccgc ggacgtcagg gttcccgtat catctcgatc cagccaagct cgtacccaaa
     1261 ccggcccgca aggcatgccc gaaaatgaaa aatttatttt attttcaata ggttgcgttc
     1321 cagtcggcgc tgtcatggaa taggaatgga gcgtaacggg cagccaacca tattcgaccg
     1381 tcagcgcatg cggcagtggt ccggaaacca tgaccgccac atcggtgggg cagccttcga
     1441 atctcaggat cgccccatct cctcgatgct tttccacgcg gtaccctctc gaaaccaacg
     1501 tttcgagttc atgacggggt ccgatataca cgacatcttc gaacgaagag ccaaaggaag
     1561 cccagcttac catggcggca gacgctacgt gctgattgct ttgcgccacg gcagcacgat
     1621 acacgggacc gtaaacctgt cggtatgggg cagccggcat gcggctcttg ccttcctcgc
     1681 tgaacaccaa tccgtgtagc tgcggcacgg aggtgaacgt gtagggaacg acgcctcctt
     1741 gctcgatggc gaaaagggtc cccacattca gggagggttc gaaatacgga taccaccggt
     1801 ccatttcgtt cccgtagcca agtcgtgtat cgacaatcat gggcagacgt ggtccgtggc
     1861 gccgaatcgg ttcccgagca agggcgagaa tatcggcgga acggttgaaa agttcgcgat
     1921 ggaaccgagt ggaccaggcc agggccgcgc ccccatgaac acaaacgagc gaaaaaacaa
     1981 cgagatacgc tgttcgggag agggtctcca aaggcagagc gacccacagc agcaaggaag
     2041 ctggagccaa aaaacgggga gagaagaact cccatgtgcc caggtgaaga ggtgcccaaa
     2101 aaaagatgag cagaagcaat gagcccacca accataaggc tcgatccgtc gcgcctgaac
     2161 gacgggtctt ccatcgccac gcgccccacc ccaccgcaaa agctgtcgcc aaggaaacag
     2221 ggatacttcg ccaataggga ccgcccacga ataggttcaa agcatcacgc atcaccatgg
     2281 caaacgatcg ttcgggggaa ggctcccctg ccggcaagga gaccgtctcg gagccaaacc
     2341 aaccgtttgc gtaggcgatt accaaaacta ccggtatccc catcaaggct agccagatga
     2401 gttctcgcca ccaacgtcgt gtcgcgtgat gaaacagcac caaagtaacc agcaccagcg
     2461 aggtaacgag cgccggaaat ggatgagcca cggcatggat cgcgagcagc agcgcaatca
     2521 acacgcggcg ttggacactc caaggcagtc gaagggccac cgccaatgtt cccagcccca
     2581 gggccatcga gatcatccag gagaaaaacc ccatatacaa cgtccattga aaagccgtgg
     2641 cgaatcccat gaccccaacg atggcacgcc gcggatcaat ggtgcgcgcg agggcccata
     2701 cgctccaccc ccacatcgat acaatcaccg aaagactgag ccgaaaagcg atctgccacc
     2761 ccaaaacgtc gtcgaggggg cagaaaatcg acgaaaaccc caaagcggca agaggccttg
     2821 ccggacgaaa gtacaacgga tagatggatc ctggatcgga aaagtgattc aggagatgac
     2881 cgaggtaaat gtgttggggg ccatcatggg tgggcacgta cgcgacgagg ttcaccgcca
     2941 ccagcgctgc caaccaggcc acaatcacgg cgtaccaaac ccttcgttgc tcagccaacc
     3001 gcgattgttt atcgtccatt cagttttatt ctctccgatg cctccctatc ttaccatgct
     3061 cgtcgcctgt cccgatccgc gctatccttg ggaatagatg tctttccttt cttccccttg
     3121 ccgtgcttat tggcttgcag ttgttctgct cggctgcggt gatgcggcat cctccccctc
     3181 ccctacccct gaaccttcca cgcttgacgc atccaccaca gcggcatcca tctcgctcga
     3241 gccatcctcc accatcacga tggcgccggg acaaaccacc gagatcacgg tcattgtcga
     3301 gcctccagac gtctatgtcg ttcgattcgc gctgctaggc gaatccctcg atgcctccct
     3361 cgacgtcgac aaacgataca catctgcgaa tggacatacc tctgtcaagc tggtggcgtc
     3421 cgaatcttcc acgacttttc gattgcgagc ctcggctgga cccactgccg tggccgaagt
     3481 cccagtgtcc gtgagcaacg agggttttgc tagcgtgctg gtgctccctt cctacaaggg
     3541 tattcgaagc acaccccaat ggacggcatc ggctctggca ggttggactt gcgcacaaat
     3601 tcccggacaa cctcccgatg atggccccat cgttggcgtt ggatcttcta aagggattcg
     3661 tctcgaatca ctacccgtcg gacctaccat cgcggtcacg ctccgctctc agcaagcggt
     3721 tgggggatgt acggaagtca ccaacctggc gcccggtgag agtcgagaag tttccgtcaa
     3781 agtatcggat atgccgatcc gttccgatga tacgaggctc gatatcacca tggcttccga
     3841 tcccctggaa gccattcctt tgctcgatga actcacggat tcgtttctgc aacggctgtt
     3901 tccttccaaa aactccgagg caaaaacgct tctcgacgcg atgcaatccg aattgcccgc
     3961 ctccgtcgac ttcgatcaag cccgactttt tggtggttgg gatgccctgg tcgcagcctg
     4021 gctcgacaaa agcggacaat ccttgcgaga gcatatccaa gcatgggtcg atgacggtct
     4081 gttgccactt cgtcaggggg aatggttgac aggccgactg accaccatcg cgggcagcaa
     4141 cacgcatgga gtactaaatc tcgagcgatt tcatggactt gcacacgaac ggacgggcat
     4201 tcccaccgag attgtcgtca gcgttgggtt cgaacccggg gacatcatga tggtcggtgg
     4261 aaaaatctcc tggctcccct ctcgtttcgt cggtgcggca gccgatgtcg gcgcaaaaaa
     4321 tattgtggga acagcggatg ccatcgacgc tttggtcgcg ctcatcggct gtaaccaact
     4381 tcccgcgttg ttgtcctcga cctatccgct ttcgccgtcg tgcgatgccc aatgcctcga
     4441 atccgcttgt catgacgccg tctcttcgct ctggaacggt gcttgtcata gttccgccat
     4501 cagcctggat accgtccagc tttccttcac catctcagga acagccacgg tcaacggcga
     4561 tgccgcggtg atcgggctcg atgcggcatg ggttggcacc caatcccacg gtgatgaaga
     4621 gatgctcgtt cgcggcaacg tccgtggtag cctcccctct acgcaatcgc gctgaaggcc
     4681 tccgtgaagc ttcctctcga atggatagtg accgctctat tttccatcgg aacgctcacc
     4741 gtattggctt tcctttttcg aaccccatcc attcccacgt ctccccatct ggaaaaacat
     4801 cacggcgaaa cgtcggccat cgccgcgcct cccatcgatt ccattccatc gctcggccca
     4861 tccttcgatg aagatgcttc ccttcccgct catgccgtat ccatcgcgag ttatacgttg
     4921 caagccaatc tcgacacgcg atcccacgtg atcgaaggaa aaggaacgat tcactgggtc
     4981 aatcgatcct caacgtccat caccgagctt cccattcacc tttatgccaa cgcgttccga
     5041 aatgaagcat cgcgttttct atccgatcgc cgtcccggag gacgtggcac ccaacgcatt
     5101 cgagacttcg ggttcatcga cgttctaagc ttcgtggttt ccagccacgg caatgacaat
     5161 ctttggccga aagccatccg ggcagatagc gcgtttccat cggacgacac ggatatgctc
     5221 gtgccactcg agcgacccgt ggctgccgga gaatccatcg acatcgaggt cgcctggcga
     5281 accaagctcc cgtccatcgt ggaacgcatg ggatatcacg atacctttca catggcggcc
     5341 cagtggtatc cgaagatcgc aaaacacgaa tccgatggcc gttttgctca cttctctttc
     5401 gatcgtcttt cggagtttta cgcggacttc ggcacctacg acatcaccat ctcggttcct
     5461 tccactttcc tcgttggtgc cacgggccct tgcgtatccg acatcatcga aggggatcgc
     5521 aggatcgtcc gtcacgtgca agccgatatc cacgactttg cgttcgcggc atgggatggc
     5581 tttcgggagg ttcgacggca ggtggatggc atcgatgtgc gactgctttt tccgccggga
     5641 tatgaacgtg tcgccacgga gcaactcgat gtgaccacgt ttggtcttcg gtattatggg
     5701 aaacgattcg gctcctatcc ctatgcaatg ctgacgatcg tacatcctcc ttcgggagcc
     5761 ggggaagcgg gcggtatgga ataccccacc ttattcacga ccggtggatc gtggtgggaa
     5821 cgtggtccca acggtatggc tcgttccgtc acgctccatg aactcgcgca tcaatacttt
     5881 caaggcatgg tcgccaccaa tgaggcgacg tggccgatgc tcgacgaagg aatcaccagc
     5941 tttgtcgagc ttgatgctct cgatacggga tggccagacg ccggagcctt ctcgctttcc
     6001 gattatggcg tttccgcggc aacgatggcc cgctggtttg gtttgaccgc gggaacggat
     6061 gacatcatcg caaaacccgc tgccgaattt tcttctggac acgcttatgc gcggctcgtt
     6121 tattggcgat cggccctgct gtggcgaacg ctcgatcgcg ttttcgacgg cacgatcatg
     6181 caagctctcg gtcgttacgc gcgtcgctat cgattcaccc atcccgagcc cgaacatctc
     6241 ctttccgccg tgcgtgacgt tgcgggtaag caggctgagg acgctcttcg catcagcttg
     6301 ttcgagcgag gctggatcga tgtagccatc aatgatgcct tctgtggttc cctatcgtgc
     6361 caagcgatcg tcagccgacg gggcaacctc gagcttcctg tcgatgtgct gtggatccac
     6421 acggatggtt cgcgtgtgat gactcgttgg gagggaaaag aagtgcaaac gacccttcat
     6481 aaggaggggc ttatccccgt ccgccatatc gtattggatc ccgaacacaa gattttgctc
     6541 gacgacgatc tttccaacaa cgcgattttc ctcggccagc gtccacgcgc gtggcgaacc
     6601 aggagttata taagttacct cgcgatcctc ggtctgcagg cggtgatgcc atgatcgcgg
     6661 tgtatcgcct tcgatggcta cccgccgtac cgtatgccat ggctggatac gcgattcgac
     6721 tcgccctggc cttcgtactg attcatccca tcgcatcggt cgtggaacgc acgttggccg
     6781 gatggccagc gaatgaacac atgatgttcc actccggatc cttgcttttc agtgaggtgt
     6841 tacgtctgca cggagttgcc tttcatggtc ttctcgaaca acttgctttg ggggcattgt
     6901 tgatgattcc gatcgggatc gtcattcacg cgttcgtgat cgcatcgttc gtcgatcccg
     6961 ataccagaga ccttaaggcc atcgcttcgt tggccatcgc acggatgctt cccgtcgccg
     7021 ttgtatccct tctctccctg cttgccatca gcgggatgat gatactttcg tgggtcattc
     7081 gcgaaggtgt tcggtccatg cttccgttcg acacgcgaac caacgatatg ctgagtttgg
     7141 gttcgatcct tgtgggagcc ttggctgtgg tggtcagcct tgtgatgacc gatctcgctc
     7201 gaatcgcaac gatacgagac aagcttgggg tcgcagaagc gattgcattg gctcttcaca
     7261 gtgttcgtaa gcgccccttc gagatattcg gagcctttgg agcgcgtgta tcggccagta
     7321 ttttcctggc gatggtggga ttgacgtttc cgctgatgtt cggaatcgaa accagcatcc
     7381 ctgcgtcatg ttggatcatc gtgcagcagg tgattgtggt ggtgatgagc tgtctcaggc
     7441 tttcatggct tgcgcgggcc gcgtcgttcc aaccccggct atcgcaacaa ggatcgatag
     7501 atctcgagat accgatgcgc tgaacgatcc caaccaaggt cgaggttcat cacgcgtttt
     7561 cgaagccggg cccagcctgg aagggcatac gacgccaccg cgcgttgcac cgcggaaacg
     7621 aggctttgtg ccgtcggttc gtcgaatacg aatccggatc cggtttccaa agtcgcatca
     7681 caatcgacga ccgtgtcgat caatccaccg gtggcatgaa ccacaggcac ggcgccgtag
     7741 cgatgggcgt agcgttggta cacgccacaa ggttcgaagc gcgaaggaac gagcacgata
     7801 tccgctcctc cgaagatccg atggagcatc ggatccgagg gtgatgagag aaatacgaaa
     7861 tcgtctttcc attttcgcgc ggcctcttcg aggcgggcgt taatagcgcc tgttccgtga
     7921 cccgccacga cgagaccgac gtcgcatcgc atgatttcag gtagagcatc gagaagaaga
     7981 tcgattccat tctgttcgtg gagagtcccg gcgaaggcga gcaacggtcg ctgcgcgcga
     8041 agtgccagcc caagttcttg aagcaatctg cctttgcatt gcgttttttg ggaaggatct
     8101 tgtccactga agcgagaggg aagatgcgga tcgatggtgg gattccagat gctgtaatcg
     8161 atcccgttga tgattccggt cagtttattt ttgtgtgcgg aaagtacccc ttcgagcccg
     8221 cggccatgag gggacttcaa gatttcgcgc gcataggttt cggagaccgt ggtcatggcg
     8281 tccgaaaata aaatcccagc tttgaggatg ttgatcttgc cgtaaaactc gacaccatca
     8341 ggatgaaata gatccccacc aatcccgagc tgatcgagtt tggaagcgtc ggacaatccc
     8401 tggtgcgtga gatcatgcaa ggtaagcacg aatttcgtgc attcttccag gtcttggttc
     8461 ccgagtcgtt tgccaaacaa aggcaccaac gacgttggcc aatcgtgcag atggacgatg
     8521 ccgaaaggtg aacccgcgcg cattcgggaa agcaacaagg caacgaccgc tcggctgaac
     8581 agagcgaaac gcatatcgtt gtcggggtag tcttcgtcac cggcgccata gataccttcg
     8641 cgatcgaaac atccaggcac ttcgagcaag gtgatatcga caccggaagg caaacgtcca
     8701 tcgaagacgg tgacttcgaa agagtcggtg
//
LOCUS       DAOSOR010000199         8534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8234_length_8534_cov_4.064748, whole genome shotgun sequence.
ACCESSION   DAOSOR010000199 DAOSOR010000000
VERSION     DAOSOR010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8534
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8234_length_8534_cov_4.064748"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..2015)
                     /locus_tag="PK710_12085"
     CDS             complement(48..2015)
                     /locus_tag="PK710_12085"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOT10506.1"
                     /translation="MYFRTEGYPRAARNMLAIVVLLGVACSAKPSAPRYPESAGPSSS
                     EQKKPEISRDPGPSPLASVAPTAEGWFDPSGLLVSLRAAERDATAQRAGLQSLADLPL
                     YDLQVSIDPGAGTYEVEQETYVTNTYGKELSTLVFRLYGNPSSANAGPTRVRVLHGDC
                     GDSVCHLRQEPPSVVTVSLQEALGPGKRLRIRFKVTGLLERVSPSQTGLLAQSLSGIL
                     GGDGGGQESTYGTMSVCEGFVSMGAFFPVLARWEGEAWERVAPRTVGDISLDALALIR
                     ARVDAPPLARVVTPGFAVRSVLFNDNQGRIRRRHEFAAAMVRDFAIFAGEGLDTVERR
                     AGEVVVRSTFRPESRKVGERVAELAASALEVFERRFGPYPYRVLEVVEAPLKEGAGGM
                     EYSGMAVLASMLYRPFGKDDPLGGLLGLLDGNDNPMVSVLDDTLEFTTAHEVAHQWWH
                     VLVGSDSSAYPFVDESLAQYSTLIYFEDRYGKERARQLADSQVRLNYIGMRLMGQGDG
                     PVMRPASSFSPLSYAGLVYGKGPFFYREARKTMGDEAFFSALRRYADDHRFRMASSKG
                     PMPYLTQHGREVSRLSQRWLEQTHGDTDLGPVTVQAVLEAVLGKQAAAMTPAIEQLLR
                     TLGTSSPDQSGPSPKDSLQMLQQLERMLDSF"
     gene            2132..3019
                     /locus_tag="PK710_12090"
     CDS             2132..3019
                     /locus_tag="PK710_12090"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT10507.1"
                     /translation="MILSAKIVPLLRKHGPDPHDGRMQEPFEQRVKRWLVAYADSFRE
                     LDALHPMLEFKLGHSARVAAYARLVAQASGWSAEETGLGESAGWLHDVGRFTQWVEYG
                     TYQDALSIDHAQRSREVVQGSSWLETLPDAHQRRLLDAIGHHNKRELPASIDQDSLPL
                     CHLVRDADKLDILDLTHRMLMDGTLQEAQPQLSGEPYVSDELIEEIQETGRGSYAKMR
                     TLMDFVVVSVAWAWDFQFAATARLVLEQEMMAKLEPYLPSTERVGQLLRQARVQLEKS
                     SAGVRLFESERKVLGEPIG"
     gene            complement(3150..3635)
                     /locus_tag="PK710_12095"
     CDS             complement(3150..3635)
                     /locus_tag="PK710_12095"
                     /inference="COORDINATES: protein motif:HMM:NF019122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10508.1"
                     /translation="MAVEVCAALKNAYAMAVGIGAGVHERMGGRASPVAHHNYEAAVF
                     AQACIEMGQLLRMLGENTDHVAHLAGAGDMLVTTSGGRSSRLGRWLGLGYSFEQAREK
                     MKGETLESAELVRVLGSALAGLVARGKLKEGSLPLMAHLFEVIVGGKTVDMPFHLFFR
                     S"
     gene            complement(3608..4198)
                     /locus_tag="PK710_12100"
     CDS             complement(3608..4198)
                     /locus_tag="PK710_12100"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10509.1"
                     /translation="MAKIVVLGAGMMGTALCTPIADAGHDVRLVGTMLDTAWVEAMKR
                     DRIHPKVGVPLPDAVRVYDLTELDAALQGVSMVGVGVSSSGVRWAAEVLGSRILPEIP
                     VIAVTKGLEQGEAGELRVLPDVFREALPEAKRGMQPCAIAGPCIAGELARRVPTAVVF
                     TGRDRAALEQFATAFPHRIIIFASRRMWWRWRYARR"
     gene            complement(4238..5287)
                     /locus_tag="PK710_12105"
     CDS             complement(4238..5287)
                     /locus_tag="PK710_12105"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HOT10510.1"
                     /translation="MSRSCFLLAAALAATVFVSSPAQAVYQCGPEKDECICGGNNPFP
                     CCDNGGNCTWWAWQSACCHWGVIVPMLGNANTWAGVAANDPAYEVLSYPVPNSIACRA
                     SGAYGHVAWVTSVNGSKITVSEMNCWGNYGVRDWSYDASFFDGGYIVLKSSLCDCSEG
                     QEQSQSCGTCGTQTRPCAANCKWGDWSACAGPDPGGPCDTGLLGACASGELRCIEGNE
                     TCSQTVTASEEVCDGVDNDCDGQVDEDDVCATPPGDAGADGSAGGSAEAGRPDSAADS
                     TADTAGEAGGTGPDAGKKPGSAWAQNNDEASGCACITTGNTKNFSNIGGVLILLALAL
                     GRRRTRSSRKRCESC"
     gene            complement(5284..5838)
                     /locus_tag="PK710_12110"
     CDS             complement(5284..5838)
                     /locus_tag="PK710_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10511.1"
                     /translation="MRRYLFLASLVLSACSPDASEPVAKQKEQALQPSNSVSRTFTDP
                     HQRWRLVLPKQAVVRTTRFDPSTPVHKVKERLEILSDDRLVLRIDIWTNPEDLSPLGW
                     IDTHATYLRNQEITLQQSYIGPENRLVVLADQPRSCQSSHIITAFLSVGKSMVSMTCV
                     DGDDPSQRTIFENALVSFAAGEQP"
     gene            6020..>8534
                     /locus_tag="PK710_12115"
     CDS             6020..>8534
                     /locus_tag="PK710_12115"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT10512.1"
                     /translation="MVPITAEASPLGRYRLWGRIASGGMADVYAGCVRGEAGFERVVA
                     IKRMASALAQDEEFVAMFLHEARLAAQIRSVHVVQTLDLGRDHEGVPYLVMDLVVGAT
                     ASRLIKRAAERSLPIPVDVVLSIIQDTCQGLHDAHEAVSREGNALGIVHRDVSPHNIL
                     IGEDGGARVADFGISKAFDSSTDATRDHMRGKLAYFSPEQARGDSLDRRSDVFSAGIV
                     AWEMLTGLRLFRAELPVRTLSRVLYGPIEPVNSWRKEVPEPVARVVERALERDLSARY
                     ATAMEFASALRKAALEAGIEPQRSAVSEFLVTCCEAEIEHIRALLRSRTSWRPDSPMP
                     SAAPRSEPSLRLDETAPDPLFSSKSEPVTQPFAMVQGQSGTFSAQLPRPRAKRLAVAL
                     ALLALLGAIAAILVIAPKGTTPAVSSLSVVQGPPTPPSVRPVLRIGTSFFRSIPSSPL
                     APAVFWSLIEPSTDGRAKAIFVRQRPSLENGLARILPGGRVEVRWELLPGLRWSDGTL
                     VRADDLLISHTLMPSSRVLDARRIDESIAVLVWSDHWAEAVEGVEPLAASVLEPHLQS
                     EGGQAAREYRNTYPTPGLGPYRIAEVRVGEWMVLEPNPYFAGPPPSMSRIELRCTQDT
                     TKLVESFERGDVDLIVPNAITVDDAEALAKRRPDAVHFRASPHYVLVQPDMNHPLLAS
                     LEVRKALLSAIDRGKLTSKLYNRTDLIAHCPAVGLGPGDVATYPFHRARALAELERLK
                     VKGVTFPLFHSHWAMDVRIATMLRDDLQAAGLKITLSPVDHVSDLVRNGHHGGLALYS
                     LRDVGPDSVAAHFNIPMVGTDYDLSVRHSGFDEGVASLLE"
BASE COUNT         1743 a         2600 c         2433 g         1758 t
ORIGIN      
        1 ccacaaaaaa tgcctagcac tcacgcgcaa gaccgaaaga gtatcgttca aaacgaatcg
       61 agcatccttt cgagttgctg cagcatctga agtgaatctt tgggcgaagg cccggattgg
      121 tctggcgacg aagtacccaa cgtacgcagc aactgctcga tcgcgggcgt catggcggcc
      181 gcttgcttgc ccaacaccgc ttcgagcacc gcttgcacgg tcaccggccc caggtccgta
      241 tccccatgcg tttgctccaa ccaacgttgc gatagacgcg atacttccct tccgtgttgc
      301 gtcaagtagg gcatcggccc tttcgaagat gccatccgaa aacggtgatc gtcggcgtaa
      361 cggcgcaacg cgctgaaaaa cgcttcatct cccatggttt ttcgcgcttc tcgataaaaa
      421 aatggccctt tgccatatac caatcccgcg tacgaaagcg gtgaaaacga ggaagcaggt
      481 cgcatcactg gaccatcccc ttggcccatc agacgcatgc cgatgtagtt caaccggact
      541 tgcgagtcgg ccaattgccg cgcacgttct ttgccataac ggtcttcgaa atagatcaac
      601 gtgctgtatt gcgccaggga ttcatccaca aacggatagg cggacgaatc actgccgacc
      661 aacacatgcc accattgatg cgccacctca tgcgcagttg taaattccag ggtgtcatcg
      721 agaacagaaa ccatgggatt atcgtttcca tcgagcaaac caagcaatcc accgagagga
      781 tcgtccttcc caaaagggcg gtagagcata cttgccaaca cagccattcc agaatattcc
      841 atccctcccg ccccttcttt cagcggtgct tcgaccactt cgagaaccct atacggatag
      901 gggccaaatc gtcgctcgaa aacctccaac gccgaggccg ccagctccgc cacgcgttca
      961 cccaccttcc ggctctcggg gcgaaaggtc gaacgcacca cgacctctcc ggcccggcgt
     1021 tcgaccgtat ccaacccttc tcctgcaaaa atcgcgaaat cgcgaaccat cgccgccgcg
     1081 aactcatgcc ttcgacgaat tcgcccctgg ttgtcgttga acaacacgct gcgcacggca
     1141 aagcctgggg tcaccacacg ggccagcggt ggcgcatcga ccctggcccg aataagcgcc
     1201 agcgcatcca aactgatatc ccccacggtg cgcggcgcga ctcgctccca ggcctcgcct
     1261 tcccatctgg caagtacggg gaaaaatgcg cccatcgaca cgaaaccctc gcatacggac
     1321 atcgtgccat aggtgctttc ttgtccgccg ccatccccgc ccagaattcc cgacaagctc
     1381 tgcgccaaca ggccagtttg cgaaggcgac acgcgctcga gaagccccgt caccttgaat
     1441 cggattcgca atcgttttcc tggcccgagc gcctcttgca acgagacggt gaccacggat
     1501 ggcggttctt gccgcagatg acagacgcta tccccacagt ccccatgcag cacgcgaacc
     1561 cgtgtgggcc cagcgttcgc gctgctcgga ttgccgtaca accgaaaaac aagcgtggac
     1621 agctccttgc cataggtgtt ggtgacgtag gtttcttgtt cgacttcgta cgtacccgcg
     1681 cctggatcga tggatacctg aagatcatac aagggaaggt ccgcaagcga ctgcagtccg
     1741 gcgcgttgcg ccgtggcatc tcgctccgcg gctcgtaagg agacgagaag ccccgaagga
     1801 tcgaaccatc cctcggcggt tggtgccacg ctggcgaggg gggaagggcc gggatcccgc
     1861 gaaatttctg gttttttttg ctcggaggac gatgggccgg ccgattcggg atagcgggga
     1921 gcggaaggct tcgcgctaca ggccacgccc agcagcacga cgatcgcgag catatttcgc
     1981 gctgcccggg ggtatccttc cgttcgaaag tacacggcta tctcctttgc gaagcacgag
     2041 tgtagcacgg acgaaagagc tgttccgtgg ggattgcccg gttttgtcag ccctttcgta
     2101 gtttccacgc gaatggacag ccattcttat catgatcctc agcgccaaaa tcgtgccgct
     2161 cttgcgcaaa cacggccccg acccccatga tggccgaatg caagagccct tcgaacagcg
     2221 ggtgaaacgc tggttggttg cgtatgcgga tagctttcgc gaattggatg cgctccatcc
     2281 catgctcgaa ttcaaactcg gtcattccgc gcgggtggcc gcttatgcac ggctcgtagc
     2341 gcaggcgagc ggctggtccg ccgaggaaac cggcctcgga gaatcagccg gatggctgca
     2401 cgatgtcggc agattcaccc aatgggtgga gtacggcacc taccaggacg ctctttccat
     2461 cgaccatgcc cagcgctccc gcgaggtcgt acaaggcagc tcatggctcg aaacgctgcc
     2521 cgatgcgcat caacgacgtt tgctcgacgc catcggccat cacaacaaga gggaacttcc
     2581 cgcttcgata gaccaagata gccttccgtt atgccatctg gttcgcgatg cggataaact
     2641 tgatattctg gacctgacgc atcggatgct gatggacggc accctgcagg aagcccagcc
     2701 ccaactatct ggcgaaccat acgtaagtga tgaattaatc gaagaaattc aagaaaccgg
     2761 tcgaggcagt tatgccaaaa tgcgaacgct catggatttc gttgtcgtat cggtagcgtg
     2821 ggcgtgggat ttccaatttg ccgccacggc tcgtctcgtg cttgagcaag aaatgatggc
     2881 gaagctcgaa ccgtatttgc catcgaccga gcgggtgggg caattgttgc ggcaggcgcg
     2941 ggtgcaattg gagaaaagca gtgcgggggt acggttattc gagtcggagc ggaaggtgct
     3001 tggtgaaccg atagggtaac gccggtggcg cggctggtgt gggacaatgg gtcatagcga
     3061 agagacgagg cgcgagggga aaagggatgg ttctcgattc cgtgccttca tcgttttccc
     3121 ttcattccct cgaacccccg gtggaccagc tacgagcgaa aaaataggtg aaacggcata
     3181 tccacggttt tccccccgac tatgacctca aaaagatgtg ccatgagcgg caacgaaccc
     3241 tctttcaact tgccccgcgc cacgagccca gcaagggcag agcccaacac acgcacgagt
     3301 tcggcgcttt ccagcgtttc ccccttcatt ttctcacggg cctgctcaaa actataccca
     3361 agcccaagcc agcgccccaa ccggctcgaa cgacctccgc tggtcgtcac cagcatatcc
     3421 cccgcccctg ccaaatgagc cacatggtct gtgttttcac caagcatgcg caataactga
     3481 cccatttcga tacacgcctg cgcaaatacc gccgcttcgt agttgtgatg ggccaccggc
     3541 gatgcccttc cccccatccg ctcatgaacg cccgctccaa tccccaccgc catcgcatag
     3601 gcgtttttca acgccgcgca tacctccacc gccaccacat ccgtcgagac gcgaatatga
     3661 taatacggtg tggaaaagcc gtcgcgaatt gctcgagagc cgccctatcc cttccggtaa
     3721 atacgaccgc cgtgggaact cgacgcgcca gttcaccagc aatacacggt cccgcgatgg
     3781 cacagggctg catgcctcgt ttcgcttccg gcagtgcttc gcggaaaacg tcgggcaaaa
     3841 cccgtagctc cccagcctcg ccttgctcca gcccttttgt cacggcgatc acaggaattt
     3901 ctggcaatat gcggctgccc aacacctccg ccgcccaacg cactcccgac gaactcacgc
     3961 ccacgcccac catcgacacc ccttgcaggg ccgcatccaa ttccgtcaga tcatatacgc
     4021 ggaccgcatc gggcaaaggc actcccacct tcgggtgaat acgatcccgc ttcattgcct
     4081 ctacccacgc cgtatccagc atcgtcccca ccagccgaac gtcgtgcccg gcatctgcta
     4141 tcggcgtgca tagggccgtg cccatcatcc cggcgccaag gactacaatc ttcgccatca
     4201 ggttgttctc cgaccggctc gcaagggtag ctcaggctca gcaggattcg caacgcttgc
     4261 gggagctgcg cgttcgccgt cggcccaaag ccagcgcaag caggatcaat accccaccga
     4321 tattcgaaaa attctttgta tttccagtag ttatgcaagc gcagccgctg gcttcgtcgt
     4381 tgttctgcgc ccaggctgac cccggttttt tgcccgcatc cgggccggtg cctcccgctt
     4441 ctcccgcggt gtcggcggta ctgtccgcgg cactgtcggg acgtccggcc tccgcggagc
     4501 ctcctgcgct accatccgca ccagcgtcgc cggggggcgt ggcgcaaacg tcatcctcat
     4561 cgacttgccc atcgcaatcg ttatccaccc cgtcgcaaac ctcttcggaa gccgtcacgg
     4621 tttgcgagca ggtttcgttg ccctcgatgc agcgaagttc accggaagcg caggcgccga
     4681 gcaatccggt atcacagggc ccgcctggat ccggtccggc gcaggcgctc cagtctcccc
     4741 acttgcaatt tgcggcgcat ggccgcgttt gcgtgccgca ggtgccacag ctttgcgatt
     4801 gctcttgtcc ctcagagcag tcgcaaaggc tgcttttcaa cacaatatag cccccgtcga
     4861 agaaggaagc atcgtaggac caatctcgaa cgccgtagtt tccccaacag ttcatctcgc
     4921 tgaccgtgat cttcgagcca ttgaccgagg tgacccaggc cacgtggccg taggcaccgg
     4981 aggcgcgaca ggcgatgctg ttgggtacgg gataggacaa cacttcgtag gcggggtcgt
     5041 tggccgcgac accagcccag gtattggcat tgccgagcat gggaacgatg acaccccaat
     5101 ggcagcaggc ggattgccac gcccaccagg tgcagtttcc cccgttatcg cagcagggaa
     5161 aaggattgtt ccctccgcaa atgcattcat ctttttccgg gccgcattgg tatacggctt
     5221 gggccggaga actgacgaac acggtcgcgg ccaaggcagc agccaggaga aaacaagagc
     5281 ggctcatggt tgttctcctg ccgcgaaaga aaccagcgcg ttttcgaata tggttcgttg
     5341 cgaaggatcg tccccatcga cgcacgtcat gctcaccatg ctctttccca ccgaaagaaa
     5401 cgcggtgatg atatggctgg attgacaaga gcgcggctgg tcggccagga cgacaagacg
     5461 gttttcgggg ccgatatagc tttgttgaag cgttatctct tggtttcgaa ggtaggtcgc
     5521 gtgggtatcg atccacccga ggggtgataa gtcctcgggg ttggtccaga tatcgatgcg
     5581 aaggacaagc cgatcgtcgc tcaagatttc gagacgctcc ttgaccttgt ggacaggggt
     5641 ggaagggtcg aaacgggttg tccgaacgac agcttgcttt ggcagaacca atcgccatcg
     5701 ctggtgagga tcggtgaagg tacgggaaac cgaattcgag ggttgcagcg cctgttcttt
     5761 ctgttttgcc acgggttccg acgcatccgg ggagcaggca gagagaacga gcgacgccag
     5821 aaagagatat cggcgcatac tccaatcatc cccttgtacg agcgcttggg caaggaagat
     5881 gggcgggggt tggaaaaaga gaagcgattt tttgatgggc tatcggggca aacaatcgct
     5941 tccagaagcg agagcgaggg agaaaaaccc ttggttggcg aagcttgtcg ggcgaccatg
     6001 gtgatggtag cgtgcgtggg tggttcccat cacggcagaa gcatccccgc tggggcgcta
     6061 tcgtctttgg gggaggattg cgtcaggggg aatggccgac gtttacgccg gctgtgtgcg
     6121 gggagaagcg ggtttcgagc gtgtggtggc catcaagcgt atggcgtcgg ccttggcgca
     6181 agatgaagag ttcgtcgcca tgtttttgca cgaagctcgg ctcgcggcgc agattcgcag
     6241 tgtgcatgtc gtgcaaacgc tggacttggg gcgcgaccac gagggggtac cgtatttggt
     6301 gatggatttg gtggtagggg ccacggcctc gaggctcatc aaacgagcgg cagaacgctc
     6361 cctgcccatt cccgtggatg tggtgttgag cattatacag gatacatgtc aggggcttca
     6421 tgatgcccac gaagccgtat cccgcgaagg aaatgcgttg gggatcgtgc atcgggacgt
     6481 ttcgccgcac aatattttga ttggagaaga tgggggagcg cgggttgcgg attttggtat
     6541 ttccaaagct tttgattctt ccacggatgc cactcgcgat catatgagag gaaaactggc
     6601 gtatttttct cccgagcaag cacggggcga tagcctggac cgtcgctccg atgtcttttc
     6661 cgccggtatc gtagcctggg aaatgttgac gggattgcgt ttatttcgcg cggagttgcc
     6721 ggtgcgaacc ctgtcgcgcg tcttgtatgg tcccatcgag cccgtgaatt catggagaaa
     6781 ggaagttccc gagccggtag cgcgggtggt ggagcgcgcg ttggagcgag atttatcagc
     6841 tcgctatgcg acggcgatgg aatttgccag tgccctgcga aaagcggcgt tggaggcggg
     6901 gatcgagccg caacgctcgg cggtgagtga gtttttggtc acgtgttgcg aagcggaaat
     6961 cgagcatatc cgtgcgctgc tccgctcgcg aacgtcgtgg aggcccgact cgcctatgcc
     7021 cagtgccgcg ccgcgctcgg agccttcctt gaggctcgat gagacagccc ctgacccctt
     7081 gttttcgagc aaatccgagc ccgtcaccca accgttcgcc atggtccaag ggcaatccgg
     7141 caccttttcc gcgcaacttc cgcggccacg ggcgaagcgc ctggcggttg cgctggcttt
     7201 gcttgcccta ctcggtgcca tcgcggccat tctggtcatc gcgcccaaag gcacgacccc
     7261 cgccgtctca tccctctcgg tcgtacaggg gcctcccaca cccccctccg tgcgtccggt
     7321 cctacgtatc ggaacctctt ttttccgcag tattccctct tcacccctcg ccccggcggt
     7381 gttttggtct ttgatcgaac catccaccga tggtcgtgcc aaagcaatct tcgtgcgcca
     7441 gcggccgtcg ctcgaaaacg gtctggctcg gattcttccc ggcggccggg tggaagtgcg
     7501 ttgggaactc ttacctgggt tacgctggtc cgatggaacc cttgtgcgag ccgacgattt
     7561 gctcatttcc catacgctca tgcccagcag tcgggtcctc gatgcacggc gtatcgacga
     7621 atcgatcgcg gtattggtat ggagtgacca ttgggcagaa gccgtcgaag gagtcgagcc
     7681 gctcgcggcc tcggtcctcg aaccgcattt acaatccgaa ggggggcaag cggcccggga
     7741 gtatcgcaac acgtatccca cacccggatt ggggccgtat cgcattgccg aggttcgtgt
     7801 cggggagtgg atggtcctcg agcccaatcc ctattttgcc ggtccgccgc cttcgatgtc
     7861 gcgtatcgag cttcgatgta cccaggacac taccaaattg gtggagtctt tcgaaagggg
     7921 cgacgtcgat ctcatcgttc ccaacgccat taccgtcgac gatgccgaag cgctggcaaa
     7981 acgtcgtccc gacgccgtac atttcagagc ctctccgcat tatgtgcttg ttcaacccga
     8041 tatgaatcat cctcttcttg cttccctgga ggtaagaaaa gccttacttt ccgcgatcga
     8101 tcgcggaaaa ctcacgagta agttatataa tcgaaccgac ctcatcgccc attgtccagc
     8161 cgttgggttg gggccagggg atgtggccac gtatcccttc catcgcgcgc gcgctttggc
     8221 ggaactcgaa aggctaaaag tcaaaggggt gacttttccg ctttttcaca gccattgggc
     8281 gatggacgtg cgtatcgcca ccatgcttcg cgacgatctt caagcggcgg ggctcaaaat
     8341 caccctttca ccggtcgatc acgtgtcgga tctggtgcga aacgggcatc acggagggct
     8401 tgcgctctat tctttgcgtg acgtcggacc ggattcggtg gccgctcatt tcaacatccc
     8461 catggttggc acggactacg acctttccgt tcggcactcc ggcttcgacg aaggggttgc
     8521 ttccctgctc gagc
//
LOCUS       DAOSOR010000200         8511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8264_length_8511_cov_5.250000, whole genome shotgun sequence.
ACCESSION   DAOSOR010000200 DAOSOR010000000
VERSION     DAOSOR010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8511
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8264_length_8511_cov_5.250000"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PK710_12120"
     CDS             <1..275
                     /locus_tag="PK710_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10513.1"
                     /translation="TGVRVLTICPGHTPTEFQLVSGVNERPVRTASQTAKDVVREALT
                     ALERGRDTLVVTGLPNRITTQAPRLIPRRWMGRMIERAFRPRVRDR"
     gene            complement(289..7290)
                     /locus_tag="PK710_12125"
     CDS             complement(289..7290)
                     /locus_tag="PK710_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10514.1"
                     /translation="MDPKDGKQGNGDGMPVEWDDEELVTGSSYFSDDELHTILEPSPD
                     RPEPPADATANDGIPRDDSGWDAVVESEPTQLQASDLEESLAFAGSDDEEPVTRRPDG
                     ADAEESGLSYEQAYEVSEPTPVPVPPTASIGAGSIRSSAPALGRVSARSIPPPPFATP
                     SMMPPPRQYDDSAPALNSYQDFRRETTRLARTRDYRSIAALHESALAHATWATSDDMQ
                     INLLLDLAKLYRDRIIDHEHAQDAFERLIARRPGHNEAMEYLREAYETKGDMRRLHDL
                     FASAVDTEWSPERRIELTRSAARIALDHLEDPSTAARDWERLLDLGDMDGQVTVELSQ
                     VYREAERWPDLGEFLENRAAACAGTTRVAILREAIEAFLSGARSPERAEALIGQVLEE
                     SPDDPIALASLASVRSQQGQWDALERVAERPMAQAPLAAKLDVLRLTAELLTGAGEHE
                     RAATAYLKVLETAPNDRNAVTARENHLRRKGDHEGLLQFLIVRANKAKDAEDKASLYE
                     RAARVADEFLGAPEMAADLFQQSVEAKPNNPRAYEALIALYERLNSVEGVTRALEGLA
                     SITQDSKARAAVLRRLGEHYAYRANNDDQAQRCWLEVAAILPDDLPVQKELNGIHRRR
                     GNFAALDEALTRQLWRTTDVQSALELSREVARNLDENLQLTTKNTRAWLHVLDLAPTA
                     QDALQALTRTFIDSDSTEVHGYLEARLSNAIREGDDAQRNDIGLQIAQLWEKRGDRLA
                     AMAAYERVRAWAPCDDRVIEPLVRLHAIDNTRAAASVLEIAAASTQGPADSREYLERI
                     MPLIPEDQPRQRFFLLHRLHRCHASTGLGEVVDAATTAEAWKDLAGLYERLAESTESP
                     EMRRAFRIHLARVCEENLANPHRAFTALQSLSLLSTNEDDRLALTRLAESTGRWEDLL
                     SVLDATLRPDLSAEERQSLLQQRAQICEQRLEDPHRAFLELQRLVQGQQGGELDDNES
                     RALEQMQRIAIEHGLLRELEAVYGELWDRAPNHEARARVARARQVIFRDHLQDSSAAV
                     QQALLILRLRPHDEVVAQEVLDASEALGVWDQTLPILEGVWRAQGDHADKLQLLADLY
                     QHKCHQPVRAAELLAEALRLSPDDDSILHKLSALGQQTGLWHRIVSAIRLAAAKAHDS
                     GRALHLAQQVAALYAEKLEDREASLDTHRWILQIWPDEIASLETVINAHREADEIEDL
                     RVRLEQWVDRVADVSRHIERWLEIGRLCRHRLHDSAGALVAFSNVIELDPSNDEAAES
                     MRALGNVSLPMGLRRKQVRLELRRASDQRRIELLQTLSQLEQQMGETDAAIAALRELF
                     ALDGGRDLALAPLAALLREAERWEELADLEEQAAGHAEPEKALEHLRASLRVSESHLD
                     DPARLERLLRHLLERMPADNDTFFHLTRLLRNTGRFQDLFNEMEGRLANFGDKHELHE
                     RLALRREIIRLLHLENNDADRAEALLRAHPEKPARPDPDDAAWLSAIMLARADHTRYI
                     EQRRRHLVKLPPRLQALVICHLAEHSDQYLKAKGRVLALYREARTIDPQNTLANDALR
                     GLGRGVKTWRSTAALLPQPDEESLSNEQRAAQLFQIGQQHLPTDSIHAISWFERAVAV
                     NPNHVEAWDALTTVALDRHDYEYAYTAALEATRAYERTTRPSVSQEASWHANRLAKTA
                     HLARLAKCDEHANELSSAAYAIDPTVPSAALFVADARFEAGFTDAAGEVYATIVQRLG
                     ESLEPKQRAHALHRLAQSKLEKGELDAAYEGLREALSTVPLFPPALGTMAEVLRKQQH
                     PVSAALHELKALLVTRDTDKRGVICRRLGELCDGELKRPDEAGAWFELAVEAGIDDKP
                     IMRRLLEHFHRTGRAQQALITIGELIESTTDPMELAELWATRGSILADHDLDAAEEAL
                     DIALSFNPAHPTALASLCNVLEGRGDYEQLAALLDARSETGTVQERVAVLHDLAEMSF
                     EKLDDEARGEMYLQRIIELAPSAFALQRLLEIVRRNPARQAEQLPLLAKLMATDLSPD
                     ASLCDRIIEASQLIFNAGHRQWTWALLSSLMGAAPVDPWTKGTLAELRREFERFDSLT
                     LIHPYVASTLGALQSPEPLQAALADLCARVFLRSDDAPGALVDARTGPGKVFERVAEQ
                     LGLTARLVRSPDGTPPAGVLAGEIPTVVIRTDLLAASPGELAFIFARGLMLARPECLA
                     LASVPENDRTLLLGALQTATNESAPPVDDPTVAALASAFSGKLSQEESMTWQRHLSDP
                     DAASRLITTVFEDMELAAMRVAAVAAGDSRTAMRAIARLNPDTRRPPGVARLEEFNSY
                     FQSVPMMGQLFAFFASEDFARVLSASA"
     gene            7532..>8511
                     /locus_tag="PK710_12130"
     CDS             7532..>8511
                     /locus_tag="PK710_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT10515.1"
                     /translation="MPLSELPPPMGTTSGASAGQESPLIRVPPPGPMSRHWAQRLARV
                     ECRALFHPEDTPSDPIVYAFSLGSNVADVDGNRYVDLAAGFGALLLGHGASRPSRSLE
                     MQLQRTWTGLGDLYPSDAKVALLERLVSLYPKGPSRALLCQSGSDAVTAAIKTAVLHT
                     DRPGIIAFEGAYHGMGYGPLAACGYRESFRSPFARQLNPFVRFAPYPHNESDLDRALQ
                     SVQHHLAHGDIAAIIVEPVLGRGGCIDPPASFLPSLFTLAHQAGALLIADEIWTGLGR
                     AGAMLASNRQNVVADILCLGKGLGGGLPISACLAAEDVMNAWTRNPGVIHT"
BASE COUNT         1737 a         2548 c         2402 g         1824 t
ORIGIN      
        1 agaccggagt acgtgttctc acaatctgcc cagggcatac acccaccgaa tttcagctcg
       61 tgtccggcgt gaatgagcgt cccgtccgaa cggcatctca aactgcgaaa gacgtggttc
      121 gcgaggctct tacagctttg gagcgaggac gagacacatt ggttgtgacg ggattaccga
      181 atcgtattac gacgcaggct cctcgattga ttccgcgaag gtggatgggt cggatgatcg
      241 aacgggcgtt tcgtccgagg gttcgagata gatgagggtg ggcagcactc aagcggatgc
      301 agaaagcacg cgggcaaaat cttcgctagc gaaaaacgcg aatagttgtc ccatcatcgg
      361 tacagactgg aaataactat tgaactcctc gagtcgcgca acgccgggag gtcgtcgagt
      421 atccggattg agtcttgcga tggcgcgcat ggccgtgcgt gaatccccgg ccgccaccgc
      481 ggcgacacgc atggcggcca gttccatgtc ctcaaaaacg gttgtgatga gcctcgaggc
      541 tgcatccggg tcggatagat ggcgttgcca cgtcatcgac tcttcttgac ttagctttcc
      601 cgaaaaagcg gaagccaaag ccgcgacagt cggatcgtcc accggcggcg ctgactcatt
      661 ggtcgcggtc tgcagggcac ccagcagcag agtgcggtca ttttcaggaa cgctcgccag
      721 ggcgagacac tcaggtcgtg caagcatgag acctctggcg aagatgaaag ctagttcccc
      781 aggagaagcc gcaagcaagt ccgtccggat gacgaccgtg gggatttccc ccgcgagcac
      841 cccggcaggt ggcgtgccat caggtgaacg aacgagccgc gcggtcaatc caagttgttc
      901 tgctacgcgt tcaaagactt tgccgggtcc ggtacgagca tccaccaaag caccaggggc
      961 atcgtcactt cgcaaaaata cgcgagcgca tagatcagcg agagccgcct gcagcggttc
     1021 aggactctgc aaggcgccga gagtcgacgc aacgtacgga tggatgagcg tgaggctgtc
     1081 gaagcgctcg aattcccttc gtaattcggc aagcgtgcct ttggtccaag ggtcgacggg
     1141 cgcggcaccc atgagcgaag agagcaaagc ccacgtccat tgtctatgtc ccgcattgaa
     1201 aatgagttgg gaagcttcga tgatgcgatc gcaaagcgat gcatccggtg aaaggtccgt
     1261 agccatgagc ttggccagca acggtagctg ttccgcttgt cgagcagggt tgcggcgaac
     1321 gatctcgaga agtcgttgga gggcgaacgc cgacggagcg agttcgatga tgcgctggag
     1381 gtacatttcg ccacgtgctt catcatcgag cttttcgaag ctcatttcgg caagatcatg
     1441 gagaacggcc acacgctctt gcaccgtacc tgtttccgat cgcgcatcca aaagagccgc
     1501 caattgctcg taatctcccc gtccttccag cacgttacat aacgatgcga gcgcggtggg
     1561 gtgtgcgggg ttgaacgaca aagcgatatc gagcgcctct tcggccgcgt ccagatcgtg
     1621 atcggcgagt atcgaacccc gagtagccca cagttccgcc aattccatcg gatcggtcgt
     1681 tgattcaatg agttctccaa tggtgatgag ggcttgttgg gcgcgtcctg ttcgatggaa
     1741 gtgctcgagc aatcgtcgca tgatgggctt gtcatcgata ccggcttcga cagcgagttc
     1801 gaaccatgca cccgcttcgt cgggacgttt gagttcacca tcacatagtt cgccgaggcg
     1861 acggcaaatg acaccacgtt tgtcggtatc tcgtgtgacg agcagagctt tgagttcatg
     1921 aagtgcggca gaaacggggt gttgctgttt gcgcaggacc tcggccatcg taccgagcgc
     1981 ggggggaaac aggggtacgg tggaaagggc ctcgcgcaaa ccctcgtacg cggcatcgag
     2041 ttcgcctttt tcgagtttcg attgagcgag acgatgcaga gcgtgtgcgc gctgtttggg
     2101 ctctaggctt tcaccgagcc gctgcacgat tgtcgcgtat acttcgccgg ccgcatccgt
     2161 gaacccggct tcgaatcgag cgtcagcaac gaagagcgcc gcggacggta cggtggggtc
     2221 gatggcgtag gcggccgagg acaattcgtt tgcgtgttca tcgcattttg ccaaccgggc
     2281 taggtgagcc gtttttgcaa gcctgttggc atgccatgag gcttcttgcg aaaccgatgg
     2341 tcgtgtcgtt cgttcataag cgcgcgttgc ctcgagagcc gcggtgtatg cgtactcata
     2401 gtcgtggcga tccaacgcaa cagtcgtgag tgcatcccac gcctccacat gattcgggtt
     2461 cacggcgacg gcccgctcga accacgaaat ggcgtgtatg gaatcggtgg gcagatgttg
     2521 ttgtccgatc tggaataatt gtgcggcacg ctgttcgttg gacagggatt cttcatcggg
     2581 ctgaggaagc aaggccgcgg tggagcgcca tgtcttaacg cctcggccca atccccttag
     2641 ggcatcgttt gccagggtgt tttggggatc gatagttcgt gcttctctgt acaaagcaag
     2701 cacgcggcct ttggccttca agtattggtc actatgctcc gccagatgac aaatcaccag
     2761 cgcttgcaac ctcggaggaa gcttgacgag atgccgacgc cgttgctcga tgtatcgagt
     2821 atgatccgct cgcgcgagca tgatcgccga tagccatgca gcgtcgtccg gatcgggtct
     2881 tgccggtttt tccggatgcg cgcgaagcaa tgcctctgca cgatccgcgt cgttgttttc
     2941 gagatgcaac aatcgaatga tttctcgtcg cagcgccaac cgttcatgca attcgtgctt
     3001 gtcaccgaaa ttggcgagac gtccttccat ctcgttgaat agatcttgga atcgtcccgt
     3061 gttacgcaga agccgggtca agtggaagaa agtgtcgttg tcggcgggca tgcgttcgag
     3121 aaggtgtcgc aacaatcgtt ctagccgcgc aggatcatcg aggtggcttt cacttacgcg
     3181 cagcgaggca cgaagatgct ccaaagcttt ttcgggctct gcatgaccag cggcttgctc
     3241 ttcgagatcg gcaagttctt cccagcgctc agcctcccgc agcagtgccg ctagaggggc
     3301 caaggcgaga tcccgaccac catcgagtgc aaacaactcc cgaagcgcgg caatggcagc
     3361 atcggtttcg cccatttgct gttcgagttg cgaaagggtt tgtagcaact cgatacgcct
     3421 ttgatcggag gcgcggcgaa gctccaatct gacttgcttt cgccgaagtc ccatcggaag
     3481 cgagacattg ccaagcgctc gcatcgattc agccgcctcg tcgttggaag gatccaattc
     3541 gatgacattg gagaacgcta cgagcgcacc ggcggagtcg tgcaaacgat ggcgacatag
     3601 cctgccaatc tcgagccatc gctcaatgtg gcgtgataca tcggcaaccc gatcgaccca
     3661 ttgctcgaga cgaactcgca agtcctcgat ttcatcggct tcgcgatgcg cgttgatcac
     3721 cgtttcgaga gaagcgattt cgtcaggcca tatttgaaga atccaacgat gggtatcgag
     3781 gcttgcttcc cgatcttcga gtttttccgc gtatagtgcc gcaacttgct gtgccaaatg
     3841 cagcgcgcga ccggaatcat gggctttcgc cgcggccagc cgaatcgcac tgacgatgcg
     3901 gtgccaaaga cccgtttgct gtcccaatgc ggatagctta tgcagaatcg aatcatcgtc
     3961 gggcgataac cgaagcgctt ccgccaataa ttcagcggct cggacgggtt gatggcactt
     4021 gtgttggtac aaatcagcca gcaactgaag cttgtctgca tgatctcctt gcgctcgcca
     4081 tactccttcc aagatgggta gtgtttggtc ccataccccc agcgcttcag aagcgtcgag
     4141 cacctcttgc gctacgactt cgtcatgagg tcgcagacgt agaatgagca acgcctgctg
     4201 tacggcggca gaagagtctt gaaggtgatc gcggaaaatg acttgtcggg ctctggcgac
     4261 acgcgcgcgc gcctcatggt tcggcgctcg atcccatagc tcgccgtaca ccgcttcgag
     4321 ttcacgaagc aatccgtgct caatggcaat acgctgcatc tgttcgaggg ctcgagactc
     4381 gttgtcatcg agttcaccgc cctgttgccc ttgcacgagc ctttgcaact cgagaaatgc
     4441 acgatgtgga tcctccagcc gctgctcaca aatctgcgcg cgttgttgaa gcagggattg
     4501 tcgttcttcg gccgaaagat ccggtcttaa cgtggcgtcg agtaccgaaa gcaagtcctc
     4561 ccaacgtccg gtggattcgg ccagacgcgt caacgccaat cgatcgtcct cattggtcga
     4621 cagcaaagaa agcgattgca aagcggtgaa cgcccgatgg gggttcgcaa ggttttcttc
     4681 gcaaacacgc gcaagatgaa tgcgaaaagc acgccgcatt tccggtgatt ccgtcgattc
     4741 ggcgagccgc tcataaagcc cggccaagtc cttccaggct tctgccgtcg ttgccgcatc
     4801 cacgacttcc cctaacccgg tcgaagcatg gcagcggtgc aatcgatgaa gcaaaaagaa
     4861 gcgctggcga ggttgatcct cagggatcag cggcatgatg cgttccagat actctcggga
     4921 atccgccggc ccttgcgtgc tcgctgccgc aatctccaac accgatgcgg ctgctcgcgt
     4981 gttgtctatc gcatgaagac gaacaagcgg ttcgatgacc cgatcgtcac aaggcgccca
     5041 agcacgaacc cgctcataag cagccatggc tgcaagccga tctcctcgtt tttcccaaag
     5101 ctgagcaatc tgtaacccaa tgtcgttgcg ctgtgcgtcg tcgccttctc gtatcgcgtt
     5161 ggatagacga gcctccaaat agccatgcac ttccgtcgag tcggaatcga tgaacgtacg
     5221 agtcagcgct tgaagagcgt cttgtgccgt gggtgcgaga tccaatacgt gcaaccacgc
     5281 tcgcgtgttt ttggtcgtca attgaagatt ttcatccaga ttccgagcta cttcacgaga
     5341 aagctcgagg gcggattgaa catccgtcgt gcgccaaagt tgacgcgtga gagcttcgtc
     5401 cagagctgca aagttgcctc tacgacgatg tattccattg agttccttct gcacgggaag
     5461 atcgtcagga aggattgcgg ctacctcgag ccaacagcgt tgcgcttgat cgtcgttgtt
     5521 ggcgcggtaa gcatagtgct ctccgagccg acgcagaacc gcggcgcgtg ctttcgaatc
     5581 ttgggtgatc gaagccaatc cctcgagggc ccgggtgacg ccttccacac tgttgagccg
     5641 ttcgtacagg gcgatgagag cctcgtaggc acgaggattg ttcggtttgg cctcgacgct
     5701 ctgttggaat aggtccgcgg ccatttccgg tgcgccaagg aattcatcgg ccacacgggc
     5761 tgcccgttcg tataacgatg ccttgtcttc cgcatctttc gctttgttcg cgcgcacgat
     5821 gagaaattgc agaagtccct cgtgatcacc ttttcgacga agatggtttt cccgagcggt
     5881 tactgcattt cggtcgttgg gcgccgtttc cagcactttc aaataggcgg tagccgctcg
     5941 ctcatgttct cccgcgcctg tcagcaattc cgccgtaaga cgcagaacat cgagcttggc
     6001 tgccaaaggc gcctgagcca tgggccgctc ggctactcgc tcgagcgcat cccactgccc
     6061 ttgttgcgat cgaacgctcg ccaaagaggc caacgcaatg ggatcgtccg ggctttcctc
     6121 gaggacttgc ccgatcaacg cctcggctcg ctccggactc ctcgccccag aaagaaacgc
     6181 ttcaatggct tcgcgaagga tggctacacg agtcgttccc gcgcacgctg ccgctcgatt
     6241 ctcgagaaac tctccaagat cgggccagcg ctccgcttcg cgatacacct gcgacaattc
     6301 caccgtcacc tggccgtcca tatcgcccag atccagaagc ctctcccagt cccgagctgc
     6361 cgtagatgga tcctccaagt gatcaagcgc tatgcgcgcc gccgaccgcg tcaactcaat
     6421 gcgacgctcc gggctccatt ccgtgtccac ggccgatgca aataaatcat gcagtcgtcg
     6481 catgtccccc ttcgtctcgt aggcctcccg aagatactcc atcgcttcgt tgtgaccagg
     6541 ccgacgcgct atcaaacgct cgaaggcgtc ctgcgcgtgt tcgtgatcaa tgattcgatc
     6601 tcgatagagc ttggcaagat cgagcagcag attgatctgc atgtcatcgg aggtagccca
     6661 cgtggcatgc gcaagcgccg attcatgcaa cgccgcgatg gaacgataat cccgcgttct
     6721 tgccaagcga gtcgtttcac gacgaaagtc ctggtaagaa ttcaacgcgg gagcagaatc
     6781 atcgtattgc ctgggaggcg gcatcatgga cggcgtggca aagggaggag gagggatcga
     6841 acgggcggaa acccgaccca aagccggcgc cgacgaccga attgacccag ctccgatcga
     6901 agccgtgggg ggaaccggca caggcgttgg ttccgacacc tcatacgctt gctcatacga
     6961 caaaccagac tcctctgcgt cggccccgtc cggacgtcgt gtgacgggct cctcgtcgtc
     7021 actccccgcg aaggcaagag actcctccaa atcagaggct tgcagttgcg tcggttccga
     7081 ttctacgaca gcgtcccaac ccgaatcatc tctgggtatg ccgtcgtttg ccgtggcatc
     7141 cgctggtggt tcgggccgat ccggcgacgg ctccaagatc gtatgaagct catcgtcgga
     7201 gaaataactc gaccccgtga cgagttcttc gtcgtcccat tcgaccggca ttccatcccc
     7261 attgccctgc ttgccgtcct tcggatccat cgtccgcatc tccgtatttc caagtgccga
     7321 gtttttcggc cggaagcctt cgaaaccgtc cgcgtctcca actacgatcg gtgacgtagc
     7381 cttgatcgtt agcacaggcg cgtagcgaag attggcaaaa actggcgaaa ttccacgagc
     7441 ggtgctccat cgtttcctgc cgcaccgacc cgaaaccatt cccaatcgtt cctaggtctg
     7501 ttttttctca ctgcctgcta cactacccgc catgcctctc agcgagcttc ctcctccgat
     7561 ggggaccacc tcgggcgcaa gcgccggtca ggaatctccc ctcattcggg ttccaccccc
     7621 aggtcccatg tcgcgccatt gggcacaacg actcgcgcgc gtcgaatgcc gtgccctgtt
     7681 ccaccccgaa gataccccgt ccgaccctat cgtctatgcg ttttccctcg gctccaatgt
     7741 cgccgacgtc gatggaaacc gctacgtaga cctagccgcc ggtttcgggg cccttcttct
     7801 cggccatggt gcctcacgcc ctagccgttc gctcgaaatg cagttgcaac gcacctggac
     7861 cggactcggc gacttgtacc cctccgatgc caaagtggca ttgctcgaac gcctggtatc
     7921 cctttacccc aaaggtccat cgcgagcact attgtgccaa tcggggagcg atgcggtgac
     7981 cgcagccatc aaaaccgccg tgctgcatac cgatcgccca ggaatcatcg ccttcgaagg
     8041 cgcctaccac ggaatgggtt acggcccctt ggcggcttgc ggctatcgcg aaagctttcg
     8101 ctccccgttt gctcgtcaac tcaacccctt cgtgcgattt gcaccctacc cacacaacga
     8161 atccgacctc gatcgagccc tacaatccgt ccaacaccat ctcgcccacg gtgatatcgc
     8221 agccattatc gtggaacccg tgctcggacg cggcggatgc atcgacccgc ctgcctcctt
     8281 ccttccttcc cttttcaccc tcgcccatca ggccggagcc cttctcatcg ctgacgaaat
     8341 ctggaccggg ctcggtagag caggcgccat gctcgcttcc aatcgccaaa acgtcgtcgc
     8401 cgatatcctc tgcctcggca aaggtctcgg cggtggtttg cccatctccg cctgcctcgc
     8461 ggcagaagac gtcatgaacg catggacccg aaatcctggc gtcatccata c
//
LOCUS       DAOSOR010000201         8509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8268_length_8509_cov_4.783653, whole genome shotgun sequence.
ACCESSION   DAOSOR010000201 DAOSOR010000000
VERSION     DAOSOR010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8509
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8268_length_8509_cov_4.783653"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PK710_12135"
     CDS             <1..571
                     /locus_tag="PK710_12135"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOT10516.1"
                     /translation="ASYAGTNTARDFVETPSQMFEEWAFRREILDTFAKHHQTGKPIP
                     DSLFNALIRSRAFGRALHTQRQVSLASLDLAYHSRPTPMETDRVFAEVMAKTQKFAYL
                     PNTHFQATFGHLMGYDAGYYSYQWALVIALDALTRFEKEGYTNAKVAGEWRRTVLEQG
                     AGDDENKLVERFLGRPHSLEAYGRYLRAQ"
     gene            complement(576..983)
                     /locus_tag="PK710_12140"
     CDS             complement(576..983)
                     /locus_tag="PK710_12140"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HOT10517.1"
                     /translation="MTFDEIEACLRADGWPVETLRDHSLRTGFQGISKAFPVFVYTEG
                     SYVVFAIVPYRKLPGDEALARTLMDTLLRLNDQMVFAKFSVDEDGDAILSVEYPMSYL
                     NESSLRDALDVLAYYANQHWEPLSAMGAVPALR"
     gene            complement(1198..2361)
                     /locus_tag="PK710_12145"
     CDS             complement(1198..2361)
                     /locus_tag="PK710_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT10518.1"
                     /translation="MNKAFDIFNPTEEHQQLRENVRRFAEAEMDGQAASYDEKESFNE
                     ALFRKFATQLGLFGLTVSEKDGGAGMDVTASVLVHEELSRFDPALTLSFLAHEVLFVN
                     NFYHCSNEQQRERYLGKVIDGTWIAGMAMTEPEAGTDVLAMRTRALRKGDRFVLNGTK
                     QFITNGPYGNVFLVYAKTGDGPKDVSAFVVESSFAGFSVGKKESKMGMRASPTSCLVF
                     EDMEVPASNLLGSEGGALVHMMRNLEIERLTLAAQSVGIALRCCDVMTQYAVAERKAF
                     GQPLAEFGQIQRLLSESYAHTQAARALVYATALRISPEHRESLGAAASKLVATTIGET
                     VARNAIQVLGGYGYTRDYPVERLLRDAILLSIGGGTNEAMQKNITRDLARAYR"
     gene            2845..3402
                     /locus_tag="PK710_12150"
     CDS             2845..3402
                     /locus_tag="PK710_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10519.1"
                     /translation="MPFALSVAFDRRLVLYPQGQASRAEHRARCSELVLWCGGDIGRV
                     IDSPSASVNAFASYILRIKGIREGTEQVMVVLTSTNPLALTVVDLRRRLETQIPSLRT
                     ARWLIYLDPESDPFFEASAIRLGHLTGPLDEAPVWLGPQDSPFLVLSTRLRNRRFVCQ
                     GLEELDESILMRSGERLLVPFRTNR"
     gene            3497..3958
                     /gene="yajC"
                     /locus_tag="PK710_12155"
     CDS             3497..3958
                     /gene="yajC"
                     /locus_tag="PK710_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOT10520.1"
                     /translation="MSHFTMDLQTVPDAPGRGEAPQPAQHTESAAPPTHGDAPPVGAG
                     GPFGGMGMLLIMFLPMILIIFLLNRSNSKKQRELESKLKKGDRVITSGGIIGRLVEIS
                     PNSKYIKLEVTSGVKIEMLKSAIQGLDTGDVAALSKDTSDKTDKDKLENKK"
     gene            3983..6070
                     /gene="secD"
                     /locus_tag="PK710_12160"
     CDS             3983..6070
                     /gene="secD"
                     /locus_tag="PK710_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOT10521.1"
                     /translation="MLQHILQFGFPALGLACAVGGYLSKPRRSSLWTAAIAGILAGVA
                     AHYESFWTMVTLVLIMLWAAITAMKVIDFAWRFKAGMVVTVFLFAVLSFWPTAWTMSG
                     GKVPCPQYIRDNVSFRLVAGLDLRGGLRLVYTVDVAEAIRDKRDRFYDEMRGMLATSF
                     GFHEGDKLPSREALAKLVDKVEMIKPKNDASVIVLKFKDPADASKIDEAFNRRFQYDM
                     QMIKDPSGTEFTYKIKTDIESHIRERAVTQAKETINRRVDELGLREASVVVRDEDIII
                     EVPGEDEKAFQEIRDIISRTARLEFKILDDDTDYIGSLKSKISDDQLPEGVSLDNMES
                     APVGKGKTNPIHYATVTKKQGETMQEARGRLKAFLEKHEVPNDRELGYGVIYDTDPDT
                     YRQTEKGWRTYFLYSRADITGDQIRDAAAMPDQDSSLGGWHVTLTFTDSGGDRFEEIT
                     GANVKRRFAIILDEKVESAPVIQTRIAGGHAQITMGASDPQTQLEDSRKLELVLRSGA
                     LPAPIMPSNEQRIGPSLGRDSIDQGVKAALVGSILVLGFMFMYYSRAGLIANLAVVAN
                     LFLQLAVLASFGASMTLPGIAGLALTIGMSVDANVLINERIREEQRAGKSPRAAVEAG
                     YEKAFSAIIDGNLTTLISALILAQYGTGAIKGFAITLMVGLVSNLFTAVFFTRVVFDF
                     LVRFRKIKTLRMG"
     gene            6083..7321
                     /gene="secF"
                     /locus_tag="PK710_12165"
     CDS             6083..7321
                     /gene="secF"
                     /locus_tag="PK710_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOT10522.1"
                     /translation="MEFFKPGRYFDFMGQKNIWFAVSVVLCLTSLVMFFTKGLNFGTD
                     FRGGTEIEIAFKKEIAPNDIRNAVQKLGFSAPDVIHVTDETNPYRFMLRVQEVSTIDD
                     AMQTKIQDALCFASASGGELPADRCPKDKQPTELKISPGGDKISLRYDKEPDLAQIAA
                     AIKTVEGVELRAAENNPQIVSPRDMKVEIQLKSKGDQLMDGLRSSLGAETVPDDPMRV
                     EWVGPKAGKLLRDAALKSVGISLLLMMAYVAFRFDMRFAPGAIVALAHDVLIVTGIFV
                     VTGREFTLTTVAALLTLVGYSINDTVVVYDRIRENLGKHRGKTFTTIINISVSEVISR
                     TILTSTTTLLSLMSFLILGSGAIKDFAFALFLGIIVGTYSSIYIASPLTEVIDRRIFG
                     GAKSSPGQKSRPRKREAAVV"
     gene            7368..>8509
                     /locus_tag="PK710_12170"
     CDS             7368..>8509
                     /locus_tag="PK710_12170"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOT10523.1"
                     /translation="MLPSAASESARRLASALRITTTFADLLERRGFGDDERTRSFLNP
                     KLGQLTLPTPMADRDAAIDRIVHAIRTSESMVLFGDYDCDGITSVTILTEIIRALGGR
                     VQPCIANRFQGGYGLSDIAVDRVLSRTPSLLITCDCGSSDHERLERLKKHGVDTVVID
                     HHLVPEERLPAVAFLNPRRPDCAFPYKHLASCGLALSLAAGLRAKLSPGLDVRQWLDL
                     VAVGTVADMVPLDGDNRALVRAGMRVLEQGNRIGFQALAELARISLANITSETLSFDV
                     APRINAPGRLGHPQDALDLLLSTDAMQARGLAAKLEAARTERKRIQEQILLEAMKDIE
                     ENQWQKAPALVLARPSWHPGVVGIVASQLVEQFGRPAVVIGFDAGVG"
BASE COUNT         1884 a         2594 c         2229 g         1802 t
ORIGIN      
        1 cgcctcgtat gccggcacca acaccgcaag ggacttcgtg gaaacgcctt cgcaaatgtt
       61 cgaggaatgg gcctttcgac gcgaaatcct cgataccttt gccaaacacc atcaaaccgg
      121 aaagcccatt cccgacagcc tattcaacgc cttgatccgc tctcgagcct tcggtcgagc
      181 ccttcacaca cagcgccaag tgtccctggc atccctcgac cttgcctacc actcgcgccc
      241 caccccgatg gaaaccgatc gcgtcttcgc agaagtcatg gctaaaacac agaaattcgc
      301 atacttaccc aacacccatt tccaggctac cttcggacat ctgatgggct acgacgccgg
      361 atactacagc taccaatggg cgctcgtcat tgccctcgat gcactgacac gcttcgaaaa
      421 agaaggctat accaacgcca aagtcgcggg agagtggcgc cgcaccgtgc tcgaacaagg
      481 cgcgggcgat gatgaaaaca agcttgtgga gcgatttcta ggacgccccc acagcctcga
      541 agcctacgga cggtatcttc gcgctcaatg acgattcacc gcaacgctgg gacggctccc
      601 atggcggaca gcggctccca atgctgattg gcgtagtaag ccagcacatc gagagcgtcg
      661 cgcagactgc tttcgttcaa ataactcatc ggatactcga ccgacaggat ggcatcccca
      721 tcctcgtcca cggaaaactt ggcgaatacc atctgatcgt tgagccgaag cagcgtatcc
      781 atcagggttc gagcaagcgc ctcgtcccca ggtagctttc gatacggaac gatggcaaag
      841 accacataag agccttcggt atagacgaag acggggaaag ccttggatat tccttggaaa
      901 cccgtgcgaa gggaatggtc gcgaagcgtt tcgacaggcc atccatcggc acgcagacag
      961 gcttcgattt cgtcaaaggt catccgcggt accttatctt tccggcagca ggggatatag
     1021 tccgccatcc ggtaggagga tgtccgccag caggtgagtt gcgcaaacca tcgagggtcg
     1081 agcgggcgaa agcgcggggg aaaaagaggg gcgtttccaa gataactatt cgtaattatt
     1141 agacattata ggctaccgga aaccttcgtt ctcgcggcgc agacggtggg acgctgctta
     1201 tcgataggcc ctcgccaggt cccgggtgat gtttttttgc atggcctcgt tggttccccc
     1261 cccaatcgat aaaaggatgg cgtcacgcaa caaccgttcc acgggatagt cccgtgtgta
     1321 gccgtaccca ccgagcacct gaatggcatt tcgcgcgacc gtttccccga tggtcgtggc
     1381 gacgagtttg gaggcggcgg ctccgaggct ttcgcgatgc tcgggagaaa tgcgaagggc
     1441 ggttgcgtag acgagggcgc gggctgcctg ggtatgggca taagactcgg aaaggaggcg
     1501 ttggatttgc ccgaattccg ccagcggttg accaaaagct tttcgctccg ccaccgcgta
     1561 ttgcgtcatg acgtcacagc aacgaagcgc gatacccacc gattgagcgg ctagggtaag
     1621 acgttcgatc tccaggttgc gcatcatgtg aacgagcgcg cctccttccg aaccgagaag
     1681 gttgctggcg ggaacctcca tgtcttcgaa gacgaggcac gaggtcggcg aagcgcgcat
     1741 gcccattttt gattcttttt ttccgacgga aaacccagca aatgagcttt ccacgacaaa
     1801 agcacttacg tctttgggcc cgtcgccggt tttggcatag accaaaaaaa cgttgccgta
     1861 agggccattg gtgatgaatt gcttggtgcc attgagcacg aatcgatcgc cttttcgaag
     1921 ggcccgggtg cgcatggcaa gcacgtcggt tcctgcctcc ggctccgtca tcgccatgcc
     1981 ggcaatccac gtgccatcaa tgacttttcc gagataccgt tcgcgctgct gctcgttgct
     2041 acagtgatag aaattgttaa cgaaaagcac ctcgtgggcg agaaaggaga gcgtcaacgc
     2101 cggatcgaat cgagaaagct cttcgtgaac caacacgctc gcggtcacgt ccatacccgc
     2161 accaccgtct ttttccgaaa ccgtgaggcc aaacaggccg agctgcgtgg caaattttcg
     2221 gaataaggcc tcgttgaacg attctttttc atcgtacgac gcggcctgcc cgtccatttc
     2281 agcctccgcg aaacgtcgca cgttctcacg caactgctga tgctcttcgg tggggttgaa
     2341 aatatcgaaa gccttgttca tgagccctcc tcgtttcatg tcgtcgaact cccacgaatt
     2401 gccagagcag ccgacacgac gaaagcggtt ttgtaccccc gtcggttaaa cgatgcaatc
     2461 gggttggcgc ccagccatgc cgctgctctg cccatcgacg ataggatcgg agttcctttc
     2521 ggtatcgaaa gagtgagcac actcgccgca ggcgatcacg gaaaccaacc aaaacccctg
     2581 gtttcgttga ctaaccgcat ccgtggagca gaataatagt catattttac caaatactcg
     2641 agagatagtt tgggtaacca agatgaacgt atggttccgg ccagcacctc gatggggctt
     2701 cataaatgtt tgaacaaccg atggattatg gcatgatgaa ttcaacgaac gcagaaatca
     2761 atcctacagc atactttaag tcttatattc tgattattcc atattcttat attaatcacc
     2821 gattcaacct cgaggattgc tgtcgtgccc tttgcgctgt ccgtagcgtt tgaccgtcga
     2881 ctcgtcttgt acccgcaagg ccaggcctcg cgtgctgaac accgggcccg atgcagcgaa
     2941 ttggtattgt ggtgcggagg ggatatcgga cgggtcatcg attcacccag cgcctcggtg
     3001 aacgccttcg cctcctatat cctcagaatc aaagggattc gcgaaggtac ggaacaggtg
     3061 atggtggtgc ttaccagcac caatccgttg gcgctgacgg tggtggatct gcgacgacgg
     3121 ctcgaaacgc agataccttc gctacggacg gcgcgatggt tgatctatct cgacccggaa
     3181 tccgaccctt tttttgaagc ttcggccatt cgattggggc atttgaccgg ccctttggac
     3241 gaggcccccg tatggctcgg cccgcaagat tccccctttc tcgtcctcag tacccgcctg
     3301 cgcaaccgtc gtttcgtctg ccagggattg gaagaattag atgagtccat tttgatgagg
     3361 tccggggaac ggcttctggt accgttccgc acgaaccgct aacgtccgcg tctcggccag
     3421 ttttttcatc ccccccattg cgtcgggttg cacgtcgcca cggaagccgc tatagcccgc
     3481 actccccgga ggaatcgtga gtcattttac catggatctt cagaccgttc cggatgcgcc
     3541 cgggcggggc gaggcccctc aacctgccca gcataccgaa agcgcagccc cccccacgca
     3601 cggggatgct ccccccgtcg gtgcaggtgg accgtttggc ggcatgggca tgttgctgat
     3661 catgttcctg cccatgatcc tcatcatctt cctgctcaat cgatccaatt ccaagaagca
     3721 acgggaactc gaatcgaaac tgaaaaaagg cgaccgcgtg atcacctcgg gtggcatcat
     3781 cggtcgtctg gtggaaatct ctcccaattc caaatacatc aagctcgagg tcacctccgg
     3841 ggtaaagatc gagatgctca aaagcgcgat tcaaggcctc gatacgggag acgtcgccgc
     3901 gctgtcgaaa gacacctcgg acaagactga taaagacaag ctcgaaaaca aaaaataatc
     3961 gcccactgac ggcttcctcg ccatgttgca acatattctg caattcggtt ttcccgctct
     4021 cggcctcgcc tgcgccgtcg gcggctacct cagcaaacct cgtcgttcct cgctctggac
     4081 cgccgccatc gcggggatcc tcgccggtgt cgcggcgcat tacgagtcct tctggaccat
     4141 ggtcaccctc gtgttgatca tgctctgggc cgccatcacc gccatgaaag tcatcgactt
     4201 cgcgtggcga ttcaaagcag gaatggtcgt caccgtcttt ctcttcgccg tgctcagctt
     4261 ctggcccacc gcgtggacca tgagcggcgg aaaagtcccg tgtccacaat atatccggga
     4321 caatgtctcc ttccggctcg tggcgggact cgacctgcga ggcggcttgc gactcgtcta
     4381 cactgtcgat gtcgccgagg ccatccgaga caaacgagac cgtttctacg acgaaatgcg
     4441 cggcatgctc gccacatcct tcggcttcca tgaaggcgac aagctgcctt cccgcgaagc
     4501 cctcgccaaa ctcgtcgaca aagtcgaaat gatcaaaccc aaaaacgatg ccagcgtcat
     4561 cgttctcaaa ttcaaagacc ccgccgacgc ctccaaaatc gatgaggctt tcaatcgtcg
     4621 tttccaatac gacatgcaga tgatcaaaga cccctcaggg accgaattca cctacaaaat
     4681 caagaccgac atcgagtccc atatccgcga gcgcgccgtc acccaggcca aggaaaccat
     4741 caaccgacgc gtcgacgaac tcgggttgcg agaagcttcc gtggtcgtgc gcgacgaaga
     4801 catcatcatc gaagtgccgg gcgaagacga aaaagcgttt caagaaattc gcgacatcat
     4861 cagcagaacc gctcgcctcg agttcaaaat cctcgatgac gacacggatt atatcggttc
     4921 cctcaaaagc aaaatcagcg acgaccaact gcccgaaggc gtctctctcg acaacatgga
     4981 aagcgctccg gtcggtaagg gcaagaccaa cccgatccat tacgcgacgg tgaccaagaa
     5041 gcaaggagag acgatgcaag aagcgcgggg tcgtctcaaa gccttcctcg agaagcatga
     5101 agtccccaac gatcgcgaac ttggttacgg cgtcatttac gatacggatc cggatactta
     5161 tcgacaaacc gaaaagggat ggcggactta cttcttgtat tcccgcgcgg atatcacggg
     5221 tgaccagatc cgcgatgcgg ccgccatgcc agaccaggat tcctctctcg gtggctggca
     5281 cgtcaccttg accttcacgg actccggtgg cgatcgtttc gaagaaatca caggcgccaa
     5341 cgtcaaacga agattcgcca tcatcctcga tgaaaaggtc gaaagcgccc ctgtcattca
     5401 aacacgcatc gccggcggcc atgctcagat caccatgggt gcttcggacc cgcaaaccca
     5461 gctcgaagac tcgcgaaaac tcgaattggt gcttcgttcc ggcgccttgc ccgctcccat
     5521 catgccttcc aacgagcagc gcatcggccc ttccctcgga cgagactcga tcgaccaagg
     5581 tgtgaaagcc gctctggtcg gatccatctt ggtgctcggc ttcatgttca tgtactactc
     5641 tcgcgccggc ctcatcgcga acctcgccgt ggtagccaac ctctttttgc aactcgccgt
     5701 tctggcaagc ttcggcgctt ccatgacgct gccaggcatc gccggcctcg ccttgaccat
     5761 cggtatgtcc gtcgacgcca acgtgctcat caacgaacgt atccgcgaag agcaacgagc
     5821 agggaaaagc ccacgggccg ccgtcgaggc aggctacgaa aaagcttttt ccgccatcat
     5881 cgacggcaac ttgacgacct tgatctccgc gctcattctc gcgcaatacg gtacaggagc
     5941 catcaaggga tttgccatca cgttgatggt cggcttggtg tccaacctat ttactgctgt
     6001 gttctttacc cgtgtcgtat tcgactttct ggttcgattc aggaagatca agacgctgcg
     6061 gatgggatga gggagccaga ccatggaatt cttcaaaccc ggtcgctact tcgacttcat
     6121 gggccagaaa aatatctggt tcgccgtcag tgtcgtattg tgcctgacct cgctcgtcat
     6181 gttcttcacc aaaggcttga actttggaac cgacttccgc ggcggtaccg aaatcgagat
     6241 cgccttcaaa aaggaaatcg cccccaacga tattcgaaat gcggtccaaa agctcggttt
     6301 cagcgctccc gacgtgattc acgtgaccga cgaaaccaac ccttaccgct tcatgcttcg
     6361 tgttcaggaa gtgtccacca tcgatgacgc gatgcagacc aaaattcaag acgctctctg
     6421 cttcgcttcc gcctccggtg gcgaattgcc cgccgaccgc tgccccaaag acaagcaacc
     6481 caccgaactg aaaatctcac ccggaggcga caaaatctcc ctgcgctatg acaaagagcc
     6541 cgatctggca caaatcgccg ccgccatcaa aaccgtggaa ggggtggaac tccgcgcagc
     6601 agaaaacaat ccgcagatcg tgagcccccg ggacatgaaa gtggagatcc agctcaagtc
     6661 caaaggcgac caactcatgg acgggcttcg ctcatccctt ggtgccgaaa ccgtccccga
     6721 cgatcccatg cgagtcgaat gggtaggacc caaagccgga aagcttctgc gcgacgccgc
     6781 cctcaaaagc gtgggtatct ctcttttgct tatgatggct tacgtcgcct tccgattcga
     6841 tatgcgcttc gcaccaggcg ctatcgtcgc gctcgcccac gatgtactca tcgtcactgg
     6901 cattttcgtc gtcaccggtc gtgagttcac cctgaccacc gtcgcggcgc tcctcaccct
     6961 cgttggatac tccatcaacg atacggtggt ggtctatgac cgcatccggg agaacctcgg
     7021 gaaacaccga ggaaaaacct tcaccaccat catcaacatc agtgtttccg aagtcatttc
     7081 ccgaacgatc ttgacgtcga ccacaacctt gctgtcgttg atgtcgtttt tgattctcgg
     7141 ctccggcgcc atcaaggact tcgccttcgc ccttttcctc ggcatcatcg tgggaactta
     7201 ctcgtctatc tacatcgcct ctccgcttac agaagtcatt gaccgtcgca ttttcggggg
     7261 agccaaatcc tcaccaggcc agaaatcgcg cccacgcaaa cgcgaagcag cggtggtgta
     7321 gcctggctca tcccccttcc cctccgtcca acgcttcgat gggattcatg ctcccgtctg
     7381 ccgcctccga atccgcccgg cgtcttgctt ccgcattgcg catcaccact accttcgccg
     7441 acttgctgga gcgtcgaggc tttggcgatg acgagcgcac acgttccttt ttgaacccga
     7501 aactcggcca gctcaccctt cccacaccaa tggccgatcg agatgcggcc atcgatcgca
     7561 tcgtccacgc catccgcacc tccgaatcca tggttttatt tggcgattac gattgcgatg
     7621 gtattacttc cgtcaccatc ctcacggaga tcattcgcgc ccttggcgga cgtgtgcagc
     7681 cttgcatcgc caaccggttc caaggcggct acgggttgag cgatatcgca gtagataggg
     7741 tgttgtcccg caccccttcg ctgttgatca catgcgattg cggctcgagc gaccacgaac
     7801 gtctcgagcg cttgaaaaaa catggcgtcg atacggtggt gatcgaccac cacctggtcc
     7861 ccgaggagcg gcttcccgcg gtagctttcc tcaatccccg aagaccagat tgtgcctttc
     7921 cttacaagca cctggcctct tgcggtctcg cgctcagctt ggctgctgga ttgcgggcca
     7981 aactatctcc cggactcgat gttcgccaat ggctcgatct cgtggcggtc ggtacggttg
     8041 cggatatggt gcccctcgac ggcgataacc gggctttggt gcgcgcgggg atgcgggtgt
     8101 tggagcaagg aaatcgtatt ggatttcaag cacttgcaga attagcgcgc atttcgttgg
     8161 cgaatatcac ctcggagact ctgtcctttg atgtggcccc gcgcatcaac gcgccggggc
     8221 gtctgggcca tccccaagat gcccttgacc ttctccttag taccgatgcg atgcaggctc
     8281 ggggtttggc agccaaactc gaagcggccc ggacggaacg caagagaatc caggaacaga
     8341 tcctgctcga ggcgatgaaa gatatcgagg aaaatcaatg gcagaaggcc ccggccctgg
     8401 tgctcgcaag accgtcctgg caccccggcg tggtggggat cgtggcttcc caattggtgg
     8461 agcaatttgg ccgtcccgcg gtcgtcattg gattcgatgc gggtgttgg
//
LOCUS       DAOSOR010000202         8507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8273_length_8507_cov_4.269285, whole genome shotgun sequence.
ACCESSION   DAOSOR010000202 DAOSOR010000000
VERSION     DAOSOR010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8507
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8273_length_8507_cov_4.269285"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="PK710_12175"
     CDS             <1..1166
                     /locus_tag="PK710_12175"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT10524.1"
                     /translation="MSNHWFPLLFLNSWEMRGDDGIGATPTIVPRGNVLFPFHELPGG
                     DESRAMKWYRIPRCVMVVGVTTACGGPVSVATVAEEPKGVRDSSAPTATLHPESVDSG
                     VAEPLEPVEAEPPCPSDMVHIEHEFCPDVDRKCLVTEYNKPNRLTLCHHFEPGSTRCK
                     TERVPLSFCIDKYEYPNRQGGHPPWMVSWYDAQATCRSLGKRTCWDWEWVAACEGPDE
                     TPFPYGWKRDNTMCNIDNLWIEPRLSAAYSNDRDVALRELSRLDQSVPSGSMPGCVSG
                     YGVYDLTGNFDEWVTRAERDGHKSRWAGLKGGAWGHVRNACRPMTTSHPPEFTYYFIA
                     FRCCKEPNGASAFVPPGSSLPPEVAPQDKAPIPVPIHPPGPSQRKVEPQRSRR"
     gene            1200..2498
                     /locus_tag="PK710_12180"
     CDS             1200..2498
                     /locus_tag="PK710_12180"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOT10525.1"
                     /translation="MSAVVVVGAQWGDEGKGKIIDYLAEFADVVARFAGGPNAGHTLV
                     VDGQKTVVRLIPSGILRPDKTCLMGPGMVIDPKRLVDEIDELSRRGVSVSGRLFISYR
                     AHLILPYHLAADVLRERGDSKDQIIGTTKRGIGPAYEDKVARRGLPMAILRDLDRAQE
                     RIEAALETWAPYFQSRKEPLPDVRDLVADLVPLAERIVPFLADTSALLEDSLRQGKTI
                     LFEGAQGTLLDIDHGTYPYVTSSSATAGGVCTGSGIGPRVISRVIGAAKAYVTRVGSG
                     PFPTELFDDTATHLQQVGGEFGSVTGRPRRTGWLDLPALRYAVRVNSLDSLVITKLDV
                     LTGLNPLRVCVAYDTPEGRTSVLPVDRFDHLQGVTPVYETLPGWDEPLDQIRAFEDLP
                     DAARQYLHFIESACGVPCDLVSIGPGRDAIIVRNSPFSSR"
     gene            2608..4977
                     /locus_tag="PK710_12185"
     CDS             2608..4977
                     /locus_tag="PK710_12185"
                     /inference="COORDINATES: protein motif:HMM:NF019145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10526.1"
                     /translation="MRRVLALFTSTLALSLAASVSAAPSRFVIDVPSDQRVSSQDPVA
                     SARKILIENTGLPSKARLVLDQHRFVGGLRVMRFQQQYDGIPVYGRGASIAVDIHGVA
                     RLASAKIETRLPDSTVANITPSAAAQTASKACGLPASASHTRLLLWPTHQGARLAYSV
                     VPPSLLPIPYVPVVIVDAHSGQVLSKLNLVRYKNLANVHEFNPVSSPSTIQVELPIGD
                     SLNVPQNDLLVSFNCVDTQKVRKVDYNGFKFDVHVCELLQNAEADETDEDFLQYKLED
                     HTSGGDPFSQLSVFYHGAKVYEYFKQFDPAFELEATSKPLFLIANLMLPAGLDTMDLN
                     KMKDTSLPLEPFSNAFSVGWDPVFGQLMSMIWPEITGGAIAFGQGNTVDYAYDGDVVY
                     HEFGHSVVGSTIKLVGWWHLDEQGASAAPGAMNEALADYFSSAITGDPATGEYASQET
                     GDDAIRHLDNTNTCPASLTGEVHFDSEFFSAALWAARSKLTTDADRFAFDEAIFTALT
                     TAASGDLDFHELAELFVTAVSSSSLGASAGDDLASQFSSRGVLPTCQRLFVFDGSPIV
                     SQAPAMANSFISAGKNMFQAGNVLPYAPGTFQVEVPLPDDATSFETSFDQLIASSGMN
                     PLDPGTPFKPALIVSFDKPLEFDWANQKTNASDPVDCEKLLSYRMGATIAIPQGASKA
                     YVMLVNKGDKDGYFTALSFTFLGETESDAGVDAQQDVQEPTEAGNTDAQESEDAKPPT
                     PSSGGLSDDDDGCGCRTVTGHSQSSWLVGLLALAGMSLMRRSNRKSHCR"
     gene            4977..5927
                     /locus_tag="PK710_12190"
     CDS             4977..5927
                     /locus_tag="PK710_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10527.1"
                     /translation="MVPTLLAGATVVLGVVLASERGPTTSSSDRLRTAAGASGRPAEC
                     RKTVRGALTIWDHAREPQRHRYCDLLGRAHGLLDSSPEAARDAASLANQIFPGKAAPH
                     VLMGRAYVRMRDDQQALAAFGKARQLDPRSIEEPGALHDLAMTQVRAGNRQEATLTYR
                     ILVPRLDLYSNVNERVHVLLEAGCLAMSRGPEYADEAISLLTLARNQPLTKHSPVVLA
                     MLGLALDRALLPEQTEAVLDLLQRAGGASALSAIDPATLDYLADPSEWLAAVAISWER
                     QDPARAVALWQQYLDSQPAEPKAARAKARMAGLRKPAGGP"
     gene            5944..6885
                     /locus_tag="PK710_12195"
     CDS             5944..6885
                     /locus_tag="PK710_12195"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT10528.1"
                     /translation="MKRHSLAAMALFLCSGFSYGREPCVWQVARDPRLATDVQALRMA
                     EQLLVRHASQDSPLASSRLREALRILERADAAHSPDPRVRFLYGRLLTRSERHEQALR
                     VLSAAIDEAPDHPSVADALFSLAICHASLGRTQEEIAAYQRLLQRQWVPERRSIVQSN
                     LAESLMFVGRIEEAIQTYREAIATDDGNILARWGLAVALDRAGDSMGAFEQVNEALVR
                     DPLADELDNPLVFFVPAYDRFWYFALWAMSRAHGEHDALLRADHWQAAAQYWQRYIEA
                     APAEDRWVAVARVRRDQCVRSLGMVHRRAHPRKGTPR"
     gene            complement(7329..>8507)
                     /locus_tag="PK710_12200"
     CDS             complement(7329..>8507)
                     /locus_tag="PK710_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10529.1"
                     /translation="HPTESARPSSMIPRDSLYAERIHWTGAPTRVVAAPLHRSMAWLL
                     SGMSVIATLFAIAGSQVQSVGTGKLVLFAAWTATFALLVRTLPVWWAQAARFTVTDRH
                     VIWQSGHFKRTVQRKGISFARITWHRRNPRVGDLDLVRAVPTGALHRRLTLTLQGIGS
                     PHRVWSIVRGAQAVTGQSDGGNVPPEQRLDEGEAVQWQAAPQRSWRTVLPLTTRRTLT
                     TALGVLCLMMGVRTIFVGASTIGRLLEAGLSWGSLGLLSLLGGIGLTVVMLVLLGVWF
                     LHVGMTRKPWMDTKTRYLLTNQRFIMVRGRQELHIHRAAIVDMADRKGLYGGHDVYLI
                     LDGPQSRALSAQGAFGPAEGVRGFRPMLQAMTAEQVQSLRAALFESTTPSSPQAPPQS
                     "
BASE COUNT         1684 a         2435 c         2502 g         1886 t
ORIGIN      
        1 cgatgagtaa ccactggttt cccttgctat ttctgaattc ctgggagatg agaggcgacg
       61 atggtatcgg agcaacacca accatcgtgc ctcgtggtaa cgttcttttt ccttttcacg
      121 aattgccggg tggggatgaa agtcgcgcca tgaaatggta tcggataccg cgttgcgtca
      181 tggtggtggg ggtcacaacc gcgtgtggtg ggcctgtttc cgtggcgacg gtggcggagg
      241 agccaaaggg ggtgcgggat agttccgcgc ccacggcgac cttgcatccg gagtccgtgg
      301 attctggcgt tgcagagccg ctcgaaccgg tcgaagccga gcccccttgc ccttcggaca
      361 tggttcatat cgagcacgaa ttctgtccgg atgtggatag aaaatgcctc gtgaccgaat
      421 acaacaaacc caatcgtctc accctatgtc atcatttcga gcccggctcc acgcgatgta
      481 agaccgagcg ggttcccttg tccttttgca tcgacaagta cgagtacccc aatcggcagg
      541 ggggtcatcc tccatggatg gtgtcgtggt acgatgccca ggccacgtgc cgaagccttg
      601 gaaaaaggac ttgttgggat tgggaatggg tggccgcttg cgaaggtccc gacgaaacgc
      661 cctttcccta tggttggaag cgtgacaata ccatgtgcaa catcgacaac ctgtggatcg
      721 agccccgtct ttccgcggcc tattccaacg atcgagacgt ggccctgcgt gagttgtcgc
      781 gtctggacca aagcgtgcct tccggctcca tgcccggttg tgtatccgga tatggtgtat
      841 acgacttgac gggcaacttc gatgagtggg tgacgcgggc ggagcgtgac gggcataagt
      901 cgcgctgggc ggggctgaag ggcggcgcct gggggcatgt ccgtaacgct tgtcgcccga
      961 tgaccacgag tcaccctccg gaattcacgt actacttcat cgcgtttcga tgttgtaagg
     1021 aacccaacgg tgcatcggcc ttcgtgcctc cgggttcctc tttgccaccc gaggttgcgc
     1081 cgcaagacaa ggctccgatt cccgtgccca tccatccacc aggtccttcc caacgcaagg
     1141 tcgaacccca acggtccagg cgttgatggg tcgtattcgc ttcatccagg agatgtccca
     1201 tgtccgctgt cgtcgtcgta ggtgcccaat ggggcgatga aggaaaagga aaaatcatcg
     1261 attatctggc ggagttcgcg gatgtcgtgg ctcggttcgc gggcggaccc aacgccggac
     1321 ataccctcgt ggtcgacggc caaaaaacgg tcgttcgtct tatccctagt ggaattctac
     1381 ggcccgacaa gacctgtctg atgggtcctg gcatggtcat tgaccccaaa cggctggtcg
     1441 acgagatcga tgaattgtcc aggcgtggtg tatccgtatc cggacggctt ttcatttctt
     1501 atcgcgcgca cctcatcctt ccttatcatc tggcagcgga tgtgctgcgc gaacgaggcg
     1561 attccaaaga tcagatcatt ggcaccacga aacggggcat tggtcctgct tatgaggata
     1621 aggtggcccg gcgcggtctt cccatggcga ttttgcgcga tctcgatcgc gcgcaagaac
     1681 ggatcgaagc ggctttggaa acatgggctc cttattttca aagtcgaaaa gagccgttgc
     1741 ccgacgtgcg ggatctcgtt gcggatctgg ttcccctcgc ggagcggatc gtgccttttc
     1801 tcgccgatac gtcggctctg ctcgaagaca gccttcgaca aggaaaaaca atcttattcg
     1861 aaggtgccca gggaaccctg ctggacatag accacggtac ctacccgtat gtgacatcct
     1921 catcggccac ggccggaggt gtttgtacgg gttccggaat tggtcctcgc gtcatatccc
     1981 gtgtgattgg tgctgccaaa gcctatgtga cccgcgtggg aagcggaccg tttcccaccg
     2041 agcttttcga tgataccgcg acccaccttc agcaggttgg cggtgaattt ggttcggtga
     2101 ccggccgccc ccgtcgtacc ggttggctcg acttgcctgc cctgcgctat gccgtgcgcg
     2161 tcaatagcct cgattccctc gtgatcacca aactcgacgt gctcacgggc ctgaacccgc
     2221 tgcgggtctg tgtcgcttac gacacccctg aaggaagaac ctccgttctt cccgtcgatc
     2281 ggttcgacca cctgcaaggc gtcactccgg tttatgaaac tctgcccgga tgggacgagc
     2341 ccctggacca aatccgtgcc ttcgaggacc tgcccgacgc cgcgcgccag tacctgcact
     2401 tcatcgaatc ggcttgcggt gtgccctgtg acctcgtgtc catcggcccg ggacgggatg
     2461 ccatcatcgt tcgcaactcc cccttctcgt cacggtagcc gccccgaatc caagctcact
     2521 gccatttcac accggttgca cgtcgagctt gcacttccta cactctgcaa ggtctgccga
     2581 atgccatcga tctcggaggt atatgccatg cgacgtgttc ttgccctgtt caccagtacg
     2641 ttggctctat cgctagcggc ttccgttagc gctgctccct ctcgtttcgt gatcgatgtg
     2701 ccatccgatc agcgcgtatc gtcgcaagat cccgtcgcct cggccagaaa aatcctcatt
     2761 gaaaacactg ggttaccatc caaagctcgt ctcgttctcg accagcatcg cttcgtgggc
     2821 ggacttcgcg tgatgcgctt tcaacagcaa tatgatggca tccccgtgta tggtcgaggc
     2881 gcatcgatcg cggtggacat ccacggtgtc gcacgtctgg cttcggcgaa aatcgaaacc
     2941 cgcttgcccg actccaccgt ggcgaacatc acaccgtccg cggcggccca gaccgcttcg
     3001 aaagcctgcg gattgcccgc ttctgcttcg catacacgcc tgctcctctg gccgacacat
     3061 caaggcgcgc gtctcgccta ctccgtcgtt cccccgtcct tgctgcccat cccttacgtt
     3121 cccgtcgtca tcgtcgacgc tcactccgga caagtcctgt cgaagctcaa cctcgttcgg
     3181 tacaagaatc tggcgaacgt acacgaattc aatcccgtct cttctccgtc gacgattcaa
     3241 gtcgaacttc cgatcggtga ctcactcaac gttcctcaaa acgacctgct cgtatcgttc
     3301 aattgtgtgg atacccagaa agttcgcaag gtcgattaca acggattcaa gttcgacgtt
     3361 cacgtgtgcg agctgttgca aaacgcggag gcggacgaaa ccgacgaaga ctttttgcaa
     3421 tacaaactcg aggatcatac gtcggggggc gatcctttct cacagctttc ggtcttttat
     3481 catggggcga aagtgtacga atacttcaag caattcgacc ccgccttcga actcgaagcc
     3541 acgtccaagc ctctgttttt gattgccaac ctcatgctgc ccgcgggcct ggacaccatg
     3601 gacctcaaca aaatgaagga tacgagcctg cctctcgaac cgttttcgaa cgcgttttcg
     3661 gtcggttggg atccggtatt tgggcagttg atgtccatga tttggccgga gattaccggt
     3721 ggcgcgattg ctttcggtca gggcaatacg gtggattatg cctacgatgg cgatgtcgtc
     3781 tatcacgaat tcggtcattc ggtcgtcggc tccaccatca agctcgtggg atggtggcac
     3841 ctcgatgagc aaggggcttc ggccgcgccg ggagccatga acgaagcgct ggcagactat
     3901 ttctcgagcg ccatcacggg cgatcccgcc acgggggaat atgcaagtca ggagacgggc
     3961 gacgatgcca ttcgtcatct ggacaacacc aatacctgcc ccgccagcct tacgggcgag
     4021 gtgcattttg attcggagtt tttctccgcg gcgttgtggg cggcgcgttc gaaattgacc
     4081 acggatgccg accggttcgc gttcgacgag gcgattttca cggcgcttac cacggccgcg
     4141 tccggggacc tcgattttca tgaactcgcg gagctattcg tgaccgcggt tagttcttcc
     4201 tctctcggcg cttccgcagg ggatgatctt gcatcgcaat tttcgtcgcg tggtgtgctt
     4261 cccacctgcc aacgcctctt cgtattcgat ggatcgccca tcgtctcgca ggccccggcc
     4321 atggccaatt ccttcatttc cgctggaaaa aatatgttcc aagcaggaaa tgtcctgccc
     4381 tatgcccctg gcacgttcca ggtggaagtg cctttgcccg acgatgcaac ctcgttcgaa
     4441 acgtctttcg accaattgat cgcttcgtcg ggaatgaatc ctttggaccc gggcacgcct
     4501 ttcaaaccag cgctcatcgt cagtttcgac aagccgctgg agtttgattg ggcgaatcaa
     4561 aaaaccaatg cttcggatcc cgttgactgc gaaaaactac tttcctatcg aatgggcgcg
     4621 acgatcgcta tcccgcaagg cgcttccaag gcgtatgtga tgctcgtcaa caagggtgat
     4681 aaagacggat atttcacggc gttatcgttc actttcctcg gtgaaacgga atcggatgcg
     4741 ggggtggatg cccagcagga tgtgcaagaa cccacggagg caggaaacac cgatgcccag
     4801 gagtcggagg atgccaagcc tcctacgcct tcgtcgggcg gcttgagcga cgacgacgat
     4861 ggttgtggtt gtcggaccgt gacaggtcat tcccagtcgt cgtggctcgt gggtttgctt
     4921 gccttggcgg gaatgagtct catgcggcga tcgaaccgaa agagtcattg tcgataatgg
     4981 ttcctacact cctggcgggt gcaacggtgg tgttgggtgt ggttcttgcg tccgagcgag
     5041 gaccgacgac atcgtcgtct gatcggttgc gcacggcggc gggagcatcg gggcgcccgg
     5101 cggagtgtcg aaagacggtt cgaggtgcat tgaccatttg ggatcatgca cgggagccgc
     5161 aacggcatcg gtattgtgat ctgctggggc gagcccatgg gttgttggac tcttcccccg
     5221 aggcagctcg cgacgccgcc tctcttgcaa accagatatt tccgggcaaa gcggcgcccc
     5281 atgtcctgat ggggcgggcg tatgttcgca tgcgcgatga tcagcaggcg ttggctgctt
     5341 tcggaaaagc gcgccaactc gatccgcgaa gcatcgaaga gcccggtgcg ttgcatgatc
     5401 ttgcgatgac gcaggtgcgc gcgggcaaca ggcaagaagc caccttgacg tatcgcatcc
     5461 tggtgcctcg actcgacctg tattcgaatg tcaacgagcg cgtgcatgtg ctgctcgagg
     5521 ccggttgctt ggcgatgagc cgtggccccg agtacgccga cgaagccatt tcgcttctta
     5581 cactcgccag aaatcagccc ctgaccaagc atagccccgt tgttcttgcc atgctgggcc
     5641 ttgccctgga ccgtgcgctt ttgcctgagc agaccgaggc cgtgttggac ttgctacaaa
     5701 gggctggagg tgcttctgcc ctttcggcga tcgatcccgc gacgttggat tatcttgcgg
     5761 accccagcga atggttggcg gcggtggcca tctcgtggga gaggcaggat ccggctcgtg
     5821 ccgtcgctct gtggcagcag tatttggata gtcagcccgc cgagccgaaa gcggcacgcg
     5881 ccaaagcacg gatggctggg cttcgcaagc cggccggcgg accatgaaca tggaaagcct
     5941 tttgtgaaac gccattctct tgccgccatg gctctttttc tatgcagcgg cttttcgtac
     6001 gggcgagagc cgtgcgtgtg gcaagtggct cgcgatccgc gtctcgcaac cgatgtccag
     6061 gccttgagaa tggccgaaca gttgctcgtt cgacacgcct cccaagacag tccccttgcc
     6121 agttcgagac tgcgggaggc cttgcgcatt ctcgagcgtg ccgatgccgc ccattcccct
     6181 gacccgcgcg ttcgcttctt atacggacga ctgctcacac gatccgaacg acatgagcaa
     6241 gccctgcgcg tattgtccgc cgccatcgac gaggctcctg accatccgag tgtcgccgat
     6301 gccctttttt ccctagcaat ttgtcatgcc agccttggac ggacccaaga agaaattgct
     6361 gcataccaac gattgctcca gcgccaatgg gttccggaac gacgttccat cgtacaaagc
     6421 aatctcgcgg aaagcttgat gttcgtgggg cgtatcgaag aggcgataca aacctatcgg
     6481 gaagcgattg cgaccgatga tggcaatatc cttgcccgtt gggggctagc ggtggccctc
     6541 gatagagcgg gtgattccat gggcgctttt gaacaagtga acgaggcgct tgttcgcgat
     6601 ccgttggcgg acgaactcga caatcctctc gtttttttcg tgcccgccta cgataggttc
     6661 tggtacttcg ccctgtgggc catgagtcgg gcccatggcg agcatgatgc ccttctccgg
     6721 gccgatcatt ggcaagcggc ggcgcagtat tggcaacgat acatcgaggc ggcgcccgcg
     6781 gaggatcgat gggtcgccgt ggcgcgcgtg cgacgtgatc aatgtgttcg aagcttgggt
     6841 atggtgcatc ggcgggctca tccaagaaag ggcacgccac ggtagtcgca gcaatcgttg
     6901 gggttcggat aggagggaag ggggccacag acgattgggc aacgctcatg actgggggga
     6961 gcgtggggcg tgtgggtgat cgttcggcga cttgaagcac ctatgtcgag tatatacaat
     7021 tatgtatata ggtgccgtgg atgatggccc tgagaggagt cgcaagaatt tgtccaccgt
     7081 ttcttccgct cttctagaag cttaaaatat ataataaata caaatagtta atcaaaatag
     7141 tatttcaagc gggaagcaaa tgcaagcggg attggagcat cgtatggtgc gagagatgat
     7201 ggaaagatga tgtgttgagt agctatacct attcgtataa tttcggtggt gaatattggg
     7261 ttgtgtccga gcgatgaggg aagggaaagt gagtaacgcg agtcgttgcg ccctacaagg
     7321 atcggaaatc agctttgggg tggggcctgt ggtgatgaag gcgtggttga ttcgaagaga
     7381 gcggcgcgaa gcgattgaac ttgctcggct gtcatcgctt gcaacatcgg acggaatccg
     7441 cggacacctt ccgcggggcc gaaggctcct tgggctgata gcgcgcgtga ttggggacca
     7501 tccaatatca ggtatacgtc gtgtccaccg tacaatcctt tgcgatccgc catgtcgacg
     7561 attgctgctc gatgtatgtg aagttcttgc cttccgcgca ccatgatgaa tcgttggttg
     7621 gtgagcaaat atcgagtctt ggtgtccatc cagggtttgc gggtcatgcc gacatgaagg
     7681 aaccacacgc ccagaaggac caacatcacg acggtcaatc cgatgcctcc gagcaacgag
     7741 agcagtccga gcgagcccca tgacagaccc gcttcgagaa gccgtccgat ggtagaggct
     7801 cccacgaaga tcgtgcgcac acccatcatc aaacacaaaa cgccaagggc cgtggtcagc
     7861 gttcgtcgag tcgtcaaagg gagcacggta cgccacgaac gttggggagc ggcctgccac
     7921 tgcaccgctt ccccttcgtc caatcgttgt tccgggggga cattgccccc gtccgactgg
     7981 ccggtgaccg cttgcgcacc gcgcacgatg gaccataccc gatgaggaga acctatccct
     8041 tgcagggtga gcgtgagacg tcgatgcaac gctccggtgg ggacggctct taccaagtcc
     8101 agatcgccca cccgcgggtt gcgccggtgc catgtgatac gggcgaacga aatacccttg
     8161 cgttgcactg tccttttaaa atgtccgctt tgccaaatca catgacgatc ggtcacggtg
     8221 aaacgcgcgg cttgggccca ccaaacgggt agtgtacgta ccaaaagcgc gaaagttgcg
     8281 gtccaggctg caaaaaggac aagctttccc gtgcccacgg attggacttg tgagcccgcg
     8341 atggcaaaga gggtggcaat gaccgacatg ccggaaagca accaggccat gctccgatgc
     8401 aacggagctg ccaccacgcg ggttggcgct cctgtccagt ggattcgttc ggcatacaga
     8461 gaatcccgag ggatcatcga actcgggcgc gccgactcgg tgggatg
//
LOCUS       DAOSOR010000203         8496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8291_length_8496_cov_4.251629, whole genome shotgun sequence.
ACCESSION   DAOSOR010000203 DAOSOR010000000
VERSION     DAOSOR010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8496
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8291_length_8496_cov_4.251629"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PK710_12205"
     CDS             complement(<1..190)
                     /locus_tag="PK710_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10530.1"
                     /translation="MATRFLPSSGGRARAIVFFCFMVVVWMLPTQARALPEQSSTPDD
                     IPGMVAVDLEDSLSQPELE"
     gene            368..2002
                     /locus_tag="PK710_12210"
     CDS             368..2002
                     /locus_tag="PK710_12210"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOT10531.1"
                     /translation="MIVATLASAQEPEGTDVPEDTAEGTAETSVPAEPKAEPESQETP
                     LNSNGSDSSGASSSTPSATLRPSKQEPCPACENAGALGYDDSVHLRYELERIVVRGNA
                     RTRERIILRYVKLRAGDIVDVDQPEWELLRYRLLGTGFFQRVSLSLRKGSQRGKVILI
                     IDVVERNTVVVSDLWMGLSADADTEGNARPLTAYAGADLAETNLGGTGITLGAGMGFA
                     QDQLALRTRFFDPSVMGSTWMATASLLYNDALEFYGNRDVLYDDPSNAIEKVRNHAVV
                     QYQRFGGLVGVGWDLSAAWQAWVDYRLERLDATLPLAASHRRGLDTEPIDFGLHRGLS
                     VLSTVRSTLQYDTRDSPILPTRGSHTTFMAEASLTPMGSDYPFQRYTLSTSKWFSLPW
                     AHVIRVGVFGGAVVGEAPIFERFYVGDFSDLLPSRMLGLNVDRRPPPNFLGTSIVEVR
                     YGDYATKVLGEYRIPLYRGSSAVYGIDFFGAGGVYAVASERDLRDPPRGYSGLARIPV
                     DLTFNLGLQIDTNAGGFTFAFSNILGFIPVRSEATP"
     gene            2002..2667
                     /locus_tag="PK710_12215"
     CDS             2002..2667
                     /locus_tag="PK710_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10532.1"
                     /translation="MRRGIALTAAMAVGTVAWVVHAQEQPTVPSKLPERRAVIVRDTT
                     SKALSASFGYRDVVDGEARRKLQSGLTTVITLAGGVFEAEGGDRPVAGTGIWQSCRVT
                     FDVWNEVYRLQITRAEGDRKAIAVNLEGVLRRCTEAHALVVQGTLTPGSKYFLAATVE
                     VNPVSQEMLEQIRRWVSRPPGTGAVSPGDSLFGSFVGLFVARIGKADRSLSFRTQTFV
                     VPP"
     gene            2918..3955
                     /locus_tag="PK710_12220"
     CDS             2918..3955
                     /locus_tag="PK710_12220"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOT10533.1"
                     /translation="MRDRNQLAPGGRVLAAVLCGLLATSAMAPYAFAQPKGSAPAAAA
                     EKQKRDAARAHYEAGKAKLDAKDFAGALTEFQAANNLIPSPQAQEKIALCYDGMNDVS
                     HAIQAYQTFVDQAKDNEKLKTEVDNAKNRIEALKNAPVAVAIVTEPASAQVQVDGQPQ
                     MGATPLDLNLTPGTHKVRVSADGFEPAEKDVEVTVGGAKFTVSFTLNPVAGPVAEPTP
                     PPPATSTAATVSTSGNKSNLPAYITLGVAGASAIVGTIFGIKALSNKSDFNDTPTTEL
                     ADDAERNALISDMTFGVAITLGVTGTVLLLSNDSSGQEQAAKDARSQKTMRLTPFVGP
                     SGGGAAATWRF"
     gene            complement(4074..5705)
                     /locus_tag="PK710_12225"
     CDS             complement(4074..5705)
                     /locus_tag="PK710_12225"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT10534.1"
                     /translation="MTPTRRDVLLSLLGASGCSLLGGKRKPFPVGTVVGADVVLGHEL
                     KGNISIPDVNQPDESTDVVIVGGGISGLSSAWRLSQDGRLSFVLLELEPELGGNARFG
                     TSPITSFPWGAHYVPVPHADNRVLIRLLSEMDELDLEQSERLGEPVGKETSLVAELEE
                     RVFYQGSWHAGLLPHAAMTPQDLEQLHRFHQEIDRWVAFRDASGRRAFTLPVANASDD
                     AQVTSLDRISMAAWMDQRGLRSVPLRWLVDYSCRDDYGCGLEDTSAWAGIFYFAARVQ
                     QPGEKSQPFLTWPEGNGRIVRHLQSLVAHRARRQQLVVDVAPRKGGLWVTMLDGPSRT
                     QHTICADHVVMAIPRFVQQRVIRSMRDRPRREPQFDTSPWLVANLTLRQRPRERGLPA
                     AWDNVLYDSPSLGYVVATHQQGRCFGPTVWTYYYALTDSNPQRARQKLFDATKEEWVE
                     VVLTDLSRAHPDLHECVQRVDLMRWGHAMIRPTPGFVWSEARRRAGESMENGKLHFAH
                     TELSSVALVEESIHHGVRAAEKVLAARFEAVTSWF"
     gene            complement(5702..7204)
                     /locus_tag="PK710_12230"
     CDS             complement(5702..7204)
                     /locus_tag="PK710_12230"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HOT10535.1"
                     /translation="MLPFLTYLTVLLVALCGLVYQFIAGTISSYLLGGGITQYSLVIG
                     VFLTALGVGAYLSKLVEQQVARRFIEVELALAIVGGLAAPSFFLAFSKAGYFRVVLYS
                     IVFLEGALIGLEIPLLVRLLRRRVQFKDLVARALAFDYIGSFLAGILFVFVFLPTLGM
                     IHTGIAFGILNAIVALFGTWLFAPSLSNPPRLRIQSLAVLAVLAGVFIGANRMTTTFE
                     SLLYSDPIVLAHQSRFQRIVVTAGRGGHHLFLDGNLQFSSVDEYRYHEALVHPVFMTR
                     TEHARVLVLGGGDGLAVREILRYPDVQHVTLVDIDQGITDLALHHPIFVKLNHGSMSH
                     SKVRIVHDDAMVWLREGTDRFDIVIIDFPDPNNYTLGKLYTTTFYRLVMARLQPEGAL
                     VVQSTSPLLARRSYWCIVATLEEVGLHVLPYHAFVPSFGEWGFALASTASIAAPSGES
                     LPVGLRYLDDNTLKSLFILSPDMAREPVQVNRLSSQALVRYYESDWRATQ"
     gene            complement(7235..>8496)
                     /locus_tag="PK710_12235"
     CDS             complement(7235..>8496)
                     /locus_tag="PK710_12235"
                     /inference="COORDINATES: protein motif:HMM:NF025158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HOT10536.1"
                     /translation="IGDQRFHVLGKVQLQHDMGGVWEEWYLGFDNGQWGWLAYAQGLF
                     MVTWKVQPSPSVPPMNQVSLEMPINFGPRGIFRIVEYKNATIASAQGELPFAPKAGDI
                     RYYADLHGPQQGFATIDWGQGTEPVEVFIGYQLLESQLRITEQVERPHQSVHLEGLQC
                     PGCGAPLKVHGGNRIERIACVYCGTISETASQQIIARQEAARTQPLIPIGASGTLAEQ
                     NYTVIGYMLKSTVFEEEVFTWSEYLLYGPGVGFRWLVDDEGTWRFVSPLNASDIDLSG
                     FPSSIGYGGKVFRMRNQNEGFTDYVLGEFYWKVQVGESTQNMDFSNGSDVVSREAGSN
                     EVHYSHAPAIPWSVIAQAFGLPNTAASSPTSSASCRGCVTAVAWVIVILVVLALLGIF
                     VCVGESGDSSPGVRGSPAYGGGWSSGK"
BASE COUNT         1897 a         2429 c         2475 g         1695 t
ORIGIN      
        1 cttccaattc gggttgagac aggctgtctt ccaaatcaac ggccaccatc cctgggatgt
       61 cgtcgggcgt ggacgactgt tcgggcaacg cccgggcttg ggtgggaagc atccaaacca
      121 cgaccatgaa gcagaaaaaa acgatggccc gcgcgcgacc gccggacgat ggcaaaaaac
      181 gggtagccat gaggtccctc cccaagacag gaagtggttt gacacacaag catagtgcga
      241 ccttgggaga aagcatctgt tgtcatccga aagcgttccg cattcggcat cccggtcggc
      301 atcgtggtag gctcctgggg tgagcagaag aaggatcgcg acgtccgggt tcggcttcgt
      361 cgccctcatg atcgtcgcga cgttggcttc ggcccaggag ccggaaggga cggatgtgcc
      421 cgaggatacg gctgagggta cggcggaaac gagcgttccc gcggagccga aagccgagcc
      481 cgaatcacag gaaactcctt tgaattcaaa cggttccgat tcatccggag cctcttcctc
      541 aacgccaagc gcaacgcttc gcccatcgaa gcaagaacct tgccccgcgt gcgagaacgc
      601 tggggccttg ggttacgacg acagcgtgca tcttcgttac gagctggaaa gaatcgtggt
      661 tcgaggcaat gcacgcacgc gcgagcggat catccttcgc tacgtcaagc ttcgcgccgg
      721 cgacatcgtg gatgtggatc aacccgaatg ggagcttctt cggtatcgtt tgctgggcac
      781 gggttttttt caacgggtga gtctttcgct gaggaaaggc tctcaacgag ggaaagtcat
      841 tctcatcatc gacgtggtcg aacgaaacac ggtggtggtg agcgatctgt ggatgggcct
      901 atccgccgat gcggatacgg aaggcaatgc gcgacctttg accgcatacg cgggtgcgga
      961 tcttgccgaa acgaatttgg ggggaacggg aatcacgctt ggggctggca tgggattcgc
     1021 gcaggaccaa ctcgcccttc gcacccgatt tttcgatcct tcggtgatgg gttccacctg
     1081 gatggccacg gcttcgctgc tgtacaacga tgctttggaa ttttatggca accgcgatgt
     1141 gctttacgac gatccgtcga atgccatcga aaaggtgcgc aatcacgcgg tcgttcagta
     1201 tcagcggttt ggtggcttgg tgggggtggg ctgggacttg agcgcggcgt ggcaagcatg
     1261 ggtggattat cgcctcgagc gcttggatgc gacacttccg ttggcagcat cgcatcgtcg
     1321 aggactcgac accgaaccga tcgattttgg tttgcatcgt gggttgagcg tgttgtcgac
     1381 ggttcgttcg acattgcaat atgacacgcg ggattcgccg attttgccta cgcggggttc
     1441 ccatacgacg ttcatggcgg aagcatcgtt gacgcccatg gggtcggact atccgtttca
     1501 gaggtatacg ttatcgacct cgaagtggtt ttcgcttccg tgggcgcatg tgatacgagt
     1561 gggcgtattt ggcggggcgg tcgtggggga ggcaccgatt ttcgagcggt tttacgtagg
     1621 ggatttcagc gatttgttgc ccagtcgcat gcttggactg aacgtggatc ggaggcctcc
     1681 tcccaacttt ctcggcacgt ccatcgtgga agtccgttat ggcgattatg cgacgaaggt
     1741 tcttggcgaa taccgaattc cgctttatcg gggttccagc gcggtatacg ggatcgattt
     1801 ttttggtgct ggaggcgtgt acgcggtcgc ctcggagcgt gacttgcgtg acccaccgcg
     1861 cggttattcc gggctcgcgc gaattcctgt cgatttgacg ttcaaccttg gcttgcagat
     1921 cgacacgaat gccggagggt tcacctttgc tttctcgaac attttggggt tcatccccgt
     1981 tcgaagcgag gcaaccccgt gatgcgtcgt ggcattgccc tgaccgcagc catggccgtg
     2041 gggacggtag cttgggtcgt ccatgcgcaa gagcaaccga cagttcctag caagttgccc
     2101 gaacgtcggg cggtgatcgt gcgggatacg acgagcaagg ctctttcggc ctcattcggt
     2161 tatcgcgacg tggtggatgg ggaagcgcgt cgcaaactac aaagcgggct tacgacggtg
     2221 atcacgctgg cgggaggagt attcgaggct gagggcggag acaggccggt ggcgggaacg
     2281 gggatttggc aatcctgtcg ggtgacgttc gatgtgtgga acgaggtgta tcgattgcaa
     2341 atcacgagag cggaaggaga tcgaaaagcg attgcggtca acctcgaggg tgtgttgcga
     2401 cgatgcacgg aagcgcatgc cttggttgtg caaggaacgt tgacaccggg gagcaagtat
     2461 tttttggcgg ccaccgtgga ggtcaatccc gtatcccaag aaatgctaga gcaaatccgt
     2521 cgatgggtca gtcgaccacc gggcaccggg gcggtatcgc caggagactc cttgtttgga
     2581 tcgttcgtgg gactttttgt ggctcgtatc ggaaaagcgg accggtctct ttcctttcga
     2641 acacaaacat tcgtggtccc accgtaaggg cgtcgacgga ttatcccgat tcggacacaa
     2701 tgggcaaaat gggcaaggag cttcaaacgg tagacgtacc gaaggcccgg acgaatgtgt
     2761 ggctctagcg cggcagacga tggttttcga tatcgaatgg atttctgtcg tcttaccttc
     2821 gacagggtag acaagggatg gggcgtgctg gtagcctggg tcgagtcttc atacattcat
     2881 caaggatccc agtttgcagc agggaggacg aacgagcatg cgtgaccgaa atcaactggc
     2941 cccgggaggt cgtgtcctcg cggccgtgct atgcggtctg cttgcgacga gcgcgatggc
     3001 gccgtacgcg tttgcacagc ccaagggaag cgcacccgcg gctgccgcgg agaaacagaa
     3061 gcgcgatgcg gcccgtgccc actacgaggc gggcaaggcc aaactcgacg ccaaagattt
     3121 cgcgggcgcc cttaccgagt ttcaagcagc caacaacctc atcccttcgc cccaagcgca
     3181 ggaaaaaatt gctctctgct acgatgggat gaacgatgtc tcccacgcca tccaggccta
     3241 tcaaactttc gtcgaccagg cgaaagacaa cgaaaagctc aagaccgagg tagacaacgc
     3301 caaaaaccga atcgaagcgc tgaaaaatgc acccgtcgcc gtggccatcg tcactgaacc
     3361 cgcctcggcg caagtgcagg tcgatgggca gcctcaaatg ggagccaccc ccctcgacct
     3421 caacctcacc cccggcaccc ataaagtccg agtgtccgcg gatggcttcg aacctgccga
     3481 aaaagacgtg gaagtcaccg tcggcggggc aaaattcacc gtctcgttta ccctcaatcc
     3541 agtggcgggg cccgtggccg aacccacgcc gcccccgccc gccaccagca ccgccgccac
     3601 cgtatcgacg tcgggcaaca aatccaatct tcccgcctat attaccctcg gcgtcgccgg
     3661 cgcgagcgcc atcgtgggaa ccatcttcgg catcaaagct ctcagcaaca aatccgactt
     3721 caatgacact cccaccaccg aactcgccga tgacgccgaa cgaaatgctt tgatctccga
     3781 catgaccttc ggcgtcgcca ttaccttagg tgtcaccgga accgttttgc tgctttccaa
     3841 tgactcgagc ggtcaagaac aagcagccaa agacgctcgc tcccaaaaga ccatgcgcct
     3901 cactcctttc gtgggtccct ccggaggagg agccgccgcc acctggcgct tctaatgcct
     3961 gctccccact tcaaaattcc ataattattt ccaatagtta tacatatatc gcgcgcccgt
     4021 gcgtgcttgt ggtgccttct ggcctcgcct cacgacaagc cgattctttt cgatcagaac
     4081 cagctcgtaa cggcttcgaa tcgcgcggcc agcacctttt ccgcggctcg cacaccgtga
     4141 tgaatcgact cttccacgag cgctacactc gaaagctcgg tgtgagcaaa gtgaagcttt
     4201 ccgttttcca tcgactcacc cgcacgcctg cgcgcttcgc tccaaacgaa tccaggcgtg
     4261 ggacggatca tggcgtgtcc ccatcgcatc aggtccacgc gctgcacaca ttcatgcaag
     4321 tccggatgag cgcgcgataa atccgtcaac accacctcga cccactcctc tttcgtggcg
     4381 tcgaacagct tctgtcgcgc ccgctgggga ttggaatccg tcagcgcgta ataataggtc
     4441 cacaccgtag gaccgaagca tcggccctgt tgatgggtcg ccaccacgta tccgagcgat
     4501 gggctatcgt acaacacgtt gtcccaggcc gcgggaagac cccgctctcg cggtcgttgt
     4561 cgaagcgtga ggttcgccac gagccagggt gacgtatcga actgcggttc ccttcgaggc
     4621 ctgtcacgca tcgaccgtat cactcgttgc tgcacgaaac gcgggatggc catcaccacg
     4681 tgatcggcac aaatggtgtg ctgggtacgg ctcggaccat cgagcatcgt cacccataaa
     4741 ccgcccttgc gcggtgccac atccaccacg agctgctgac gtcttgcccg atgggccacc
     4801 agcgattgca agtgccgtac gattcgacca ttgccctccg gccaggtaag aaacggctgt
     4861 gatttttctc cgggttgttg gacccgcgcg gcgaagtaaa atatccccgc ccacgcgctg
     4921 gtatcctcga gcccgcagcc gtaatcatcg cggcacgaat aatcgaccag ccaacgcaac
     4981 ggcacgcttc gcaaaccccg ctgatccatc caggcagcca tggatatgcg atcgaggctc
     5041 gtcacctgcg catcgtcgga ggcgttggca acaggcagcg tgaaggctcg tcttccagag
     5101 gcatcccgaa acgctaccca tcggtcgatt tcttggtgga atcgatgaag ctgttcgaga
     5161 tcctggggtg tcatggccgc atgaggcagc aatcccgcgt gccaactacc ttgataaaac
     5221 acgcgctcct ccagctctgc caccagcgag gtttcttttc ccacgggttc tcccaaccgc
     5281 tcgctttgct cgagatcgag ttcatccatc tcgctcaaca agcgaatcaa cacgcggtta
     5341 tccgcgtggg gtacggggac ataatgcgcc ccccacggaa acgaggtgat gggcgacgta
     5401 ccaaatcgcg cattacctcc aagctccggt tccaattcca acaaaacgaa ggacaaccga
     5461 ccgtcttggg atagtctcca cgccgaagac aaaccggata ttcctcctcc gacaatgacc
     5521 acatccgtgg attcatcggg ctggttgaca tccggtatac ttatatttcc cttcaattca
     5581 tgtcctagaa caacatcggc ccccaccaca gtccccaccg gaaatggctt tcgcttacca
     5641 ccaagcaagc tacatcccga agcacccaat agggatagta agacatccct tcgcgtcggt
     5701 gtcattgggt cgccctccaa tccgattcat aatatcggac caacgcctgg ctgctcaatc
     5761 ggttgacttg caccggctcc cgcgccatgt ccggcgataa aataaacaat gatttcaatg
     5821 tgttatcatc tagatagcgc aaccccacgg gcagggactc ccccgaggga gcagcaatgg
     5881 aagccgtgga cgccagcgca aatccccact ccccgaacga tggcacgaac gcatggtacg
     5941 gaagaacatg caatcccact tcttccaacg tcgccacgat gcaccaatac gatcgtcgcg
     6001 ccagcagcgg cgaggtggat tgcaccacca gggctccctc aggttgcaga cgagccatca
     6061 ccaaccgata aaaggtcgtc gtatacagct ttcccagcgt gtaattgttc gggtcgggaa
     6121 agtcgatgat cacgatatcg aagcggtccg ttccttctcg aagccacacc atggcatcgt
     6181 cgtggacgat ccgtaccttc gaatgactca tcgagccgtg gttgagcttc acgaaaatgg
     6241 gatgatggag cgccaggtcg gtgatgccct gatcgatatc gacgagcgtg acgtgctgca
     6301 cgtcggggta acggaggatt tcgcgtacgg caaggccatc gccgcctccc aagaccagca
     6361 cacgcgcatg ctcggttctg gtcatgaata cgggatgcac cagcgcttcg tggtatcgat
     6421 actcgtcgac agaagagaac tgtaagttgc catcgagaaa tagatggtgg ccgccccgtc
     6481 ccgccgtcac gacgatgcgt tggaatctcg attgatgggc gagcacgatg gggtcggaat
     6541 acaggaggga ttcgaaagtg gtggtcattc gattggcgcc gataaatacg ccagcgagca
     6601 ccgcaagcac tgcaagggac tgtatacgaa ggcgaggggg attggacagg gaaggagcaa
     6661 aaagccaagt tccgaacaag gcaacgatcg cattgagaat gccgaaagca attccggtgt
     6721 ggatcatgcc aagggtgggc aaaaaaacga atacaaacag aattccagcg agaaaggagc
     6781 cgatatagtc gaaggccagc gcgcgagcga ccagatcttt gaattgcaca cgacgtcgca
     6841 gcaatcggac gagcagcggg atttccagac caatgagagc gccttcgaga aagacgatgg
     6901 agtaaagaac gacacgaaaa taacctgctt tcgaaaacgc gagaaaaaac gatggagcgg
     6961 cgagtcctcc gacaatcgca agcgcaagct cgacttcgat gaatcgtcgg gcgacctgct
     7021 gttcgacgag tttggacaga taggctccca cacccagggc ggtgagaaac acgccgatca
     7081 ccaaggaata ctgtgtgatt ccacctccga gcaggtagct cgaaatggtg ccggcgatga
     7141 attgatacac cagaccgcaa agagccacca acaggacggt caggtatgtg agaaacggca
     7201 acatgatgtg aggcgatggg atgtttcgcg ggccctattt tccactgctc cagccaccac
     7261 cgtaggcggg cgaaccgcga acgccaggcg acgagtcacc cgactccccc acacacacga
     7321 atatgccgag gagcgcaagg acaacgagga taacgatcac ccatgcgacc gcggtcacgc
     7381 agcctcggca cgacgccgat gaagtgggag aggaagctgc ggtgttggga aggccgaagg
     7441 cctgtgcgat gaccgaccat ggaatagcgg gggcgtggga atagtgaacc tcgttggaac
     7501 cggcttcgcg cgagacgacg tcggaaccgt tggaaaagtc catgttttgg gtggattcgc
     7561 cgacctgaac cttccaataa aattcgccga gcacgtagtc cgtgaagccc tcgttctggt
     7621 tgcgcattcg aaaaaccttt cctccatagc cgatggagga aggaaagccg gatagatcga
     7681 tgtccgacgc attcaatggg gacacgaatc gccaggtgcc ttcgtcgtcc acgagccagc
     7741 gaaaccccac gcccggaccg tagagaagat actcgctcca ggtgaagact tcttcttcga
     7801 aaacggtgga tttgagcatg tagccaatca ccgtgtagtt ctgttccgca agcgttcccg
     7861 aggcgccgat gggaatgagg ggttgggttc tcgccgcttc ttgtcgagcg atgatttgtt
     7921 gcgatgcggt ctccgagatc gtgccgcaat acacgcaagc gatgcgttcg atgcggtttc
     7981 ccccatgcac tttcagaggg gcgccgcaac cgggacattg aagaccttcg agatgaacgg
     8041 attggtgggg acgctcgact tgttcggtga tgcgcagttg tgattcgagc agttgatatc
     8101 cgatgaatac ctcgaccggt tccgtgcctt gcccccaatc gatcgtggca aacccctgtt
     8161 gtggtccgtg caaatccgcg taatagcgga tgtcaccggc cttgggagcg aagggcaatt
     8221 caccttgtgc cgaggcaatg gtggcgtttt tgtattcgac gatgcgaaag atgcctcgcg
     8281 ggccgaagtt gatgggcatc tcgaggctga cttgattcat gggaggcacg gatggggagg
     8341 gctgcacctt ccaggtgacc atgaacagcc cctgggcata cgcgagccaa ccccattggc
     8401 cgttgtcgaa gccgagatac cattcctccc aaacgcctcc catgtcgtgc tgcagttgga
     8461 cttttcccag cacgtgaaag cgctgatcac cgatgg
//
LOCUS       DAOSOR010000204         8486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8297_length_8486_cov_3.921599, whole genome shotgun sequence.
ACCESSION   DAOSOR010000204 DAOSOR010000000
VERSION     DAOSOR010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8297_length_8486_cov_3.921599"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..2559)
                     /locus_tag="PK710_12240"
     CDS             complement(19..2559)
                     /locus_tag="PK710_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10537.1"
                     /translation="MSPLYFDLAQRRRLAMYLALALLAASTLHLEVLITKLFSNKLEH
                     HYTFAVISIALLGFGVGGVYVHLLSHRYSPEKEADPLRLWPWALGYAASVPAVVALFL
                     VLPLDPAFPGLVGLISLPVYFLLFAVPFFFAGVCVSALLVAKGMRPTRVYFWDLFGAG
                     MGAVLGPLGLSLVGGYGSVVAASLLGFVGALILRSLEPKRRLRELALGTTAMALSCSA
                     VLLVPRVLQSTLHFDMVSFKHAPLKAEFMTFGGAVQTYWNPIARIDVSPTGESHIGGF
                     RYGLSRKDWSEPLWGRMVLVDGSANTRQFALGEKPTREGMLGRTLWAIPYVLRPETER
                     VLVLGPGGGIDLLVGKAYEIDEIDAAELNPDMYSLLLGRPEDPERLAYTRFLKSDDIT
                     DVRIFQAEARHFVRSQAGQRPYDVVLASGVDTLTAIQTAGNALSENYLYTKDAVKDYL
                     SVLSPRGQLALTHWHLEPPTLALRMFATFLEVLDEQGVDDPGRRICVVSEGFWENAIV
                     KKGEDYTQQEVERLRQFAADAGFQVLWDPFIDGDAIVKRPGDVMFRRLGRASRAERQA
                     LLEAYPYDVRPVTDDRPYFYWIRGKRGAPSPSGWLYPMASAQWLLVLALAAAVGMVVV
                     PWLVARRRGQDGRGALRVMPYFLLCGFAFILAENGLFLLLSLFVGGPLYSLSIVLPSL
                     LMGYGLGSLVVLQVVSANRAGALRLAMIYAAAFFLLWVFAHWAVPAGMGWSMETRMGT
                     AVGVVIAFGAVLGLAVPWYMEILKVRSQADRSLAWLWGMSAAANVVGAMLFVPICERL
                     GVQGTFFLAAACYVTALGWAAVHGGPDSVLAPLRSRFFTVGKKIANEG"
     gene            2781..4481
                     /locus_tag="PK710_12245"
     CDS             2781..4481
                     /locus_tag="PK710_12245"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOT10538.1"
                     /translation="MKPKNCHWFALPFVGMVILASACGDGESSGTVVSNAGTGGGKTS
                     DAGQESGVGGYGGTGGDAATGGTGASAGTGGSVGPGGTGGGTSGSGGAEVGGSAGQAG
                     SSGGAAGNAGSEGGSGGSPPPPCTTRITYGSSWIHGDNHPDDFDIASGIITWDGSCQA
                     DGGNSYATLSNGWKPYFKGKGTCVIALDYEGCQGVPASCESRVTYGPKWMPAPNHPDY
                     FDDVQGALTWNGACHNDGSASYATLSNGWKPHFSGNSACDLAFRYTQCGGLYVNPVVG
                     KDCPDPGVVFDGTQYVMACTGGGSGDAFPLRHSDDMVHWKWKGYIFPTKDRPSWAVSH
                     FWAPEIHKIGSKFVAYFTAKHKNGALSIGAATASAALGPYKDIGKPLLNNPSMGLIDA
                     THYEDPQGKHYLLWKEDGNAVGKPTPIYGQELASNGLSLVGSPVKLITNDMAWEGALV
                     EGPWLIDHGGYYYLFYSANGYASASYGVSVARASSPLGPYKKAPAPLLRTAGAWAGPG
                     HGSILTTPRGETWHIYHSWQAGHVGKDPGRVVLIDRVFWDGDWPTMLGAPSSVSLPPP
                     "
     gene            4635..5909
                     /locus_tag="PK710_12250"
     CDS             4635..5909
                     /locus_tag="PK710_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOT10539.1"
                     /translation="MTPTTHFDLTPWLCLVLGSIALTSTPLGCKSDHPPSPNNTAAGS
                     ARASAPKVSTSSARKPKVPPATAPGPSWNPSVGTRQDITDLAADAYHVAIGDVDGDGQ
                     GDLVFVDGQRIRVVTPSGRLVGEAEVSAGIQVLRTTDLDGDGRAEIVAGWGRSRHHPT
                     ATTRVTVHRLQGNTLREEVIVAPTTTRPDVATIVPTAKNSLAIAYFDSKYEVNLVHAH
                     RQQARWTLETLGQVRMAMSLAWGDVDGDGSSDLVVGRVYGETQGGDGDAFILKAKGQR
                     TALPTLRGVRGLAVADMDGDGKSEIYLGDGWHQDYGKQAKGLLSRITWAGGEYRKEVV
                     EDTAGQYTLWDIFPADIDGDGSKELITRGSAYVRAYRFRKGRWQAVAFGGMSRGIAVG
                     PLDGSAGDEVVLLGNRCEVVSLLDVNWADIER"
     gene            5912..7303
                     /locus_tag="PK710_12255"
     CDS             5912..7303
                     /locus_tag="PK710_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10540.1"
                     /translation="MRRFLLLAATGLGVSSGCDSVGVANPSRPASSVHEAFGVQPGAV
                     REAFDGPKAGDSRQLTQQARLVDEGQDPPWRWDEQVARVLDSTTLARELGYRPPRGTE
                     AYAAIIQHNELGLSYAFFDYDKGAFSQSFWPASTVKVLAALAALDWLRIHGFSGQAEV
                     RWRNGFSDRVNAIVDRSVRLSSNEDYDRTLQIAGLDHLNGRWLTAERGFPNTVIQAAY
                     AHSNVRTPPSFTLTEGDKQWVVPSRTTGPLDKCSGARVQCATLFELTEAVRRVVLRDL
                     IPPQERFELSSEDSNALQEALCQAKPSFFARGVRAVLGPDARICHKPGWVPGKDYLDH
                     GVMEAADGRRFLLAAAMPDPGDGSAQGRLPVLAEKILRVITRLEMEPFYLQPTAGAEI
                     RVKKEAGGLRVQGEGFDEVQVYIDGTGVDRVRRDEETFFAEVTTESWQDKLMTVRGYR
                     QGAIVGVRNLVLP"
     gene            complement(7745..>8486)
                     /locus_tag="PK710_12260"
     CDS             complement(7745..>8486)
                     /locus_tag="PK710_12260"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT10541.1"
                     /translation="IFIDELDAVGRRRGSGVGSAHDEREQTLNQLLSCMDGFETDDRV
                     VVIAATNRPDILDQALVRPGRLDRRIRIPLPDAETRLAILRIHTAPMPLADDVSLQKL
                     ADRCEGVNGAMLENLANEAALLAVRRCRAQGEKISVAREDFEHALQKISKITDAFDSL
                     DQVLVESATQLAKPSGPVVVEATLHDGTRVQGELVWVDGTFLKLKHNGESRIVAKRQI
                     HTLTALSGTESVRELVADPWAAQPTDVA"
BASE COUNT         1839 a         2453 c         2540 g         1654 t
ORIGIN      
        1 cacgacaggc ggtgggggct agccctcgtt cgcgatcttt tttccgacgg taaaaaaacg
       61 cgaacgaagc ggggccagca ccgagtcggg gccgccatgg acggctgccc agcccaaggc
      121 agtcacgtaa cacgccgctg ccaaaaaaaa cgttccttgc acacccaagc gctcgcaaat
      181 cggtacgaac aacatcgctc ccaccacgtt ggccgcggcg ctcatccccc acaaccacgc
      241 cagactacgg tccgcttggc ttcgaacttt caggatctcc atgtaccacg gcacggcaag
      301 gcctagcacc gcgccaaagg caatcactac ccccaccgca gtccccatcc tcgtttccat
      361 ggaccaaccc atcccggcgg gcaccgccca atgggcaaaa acccacagca gaaaaaacgc
      421 cgccgcatag atcatcgcca accgaagggc ccctgcccgg ttggccgaaa ccacctgcag
      481 caccaccaga ctgcccaatc cgtagcccat caacagcgat ggcaacacaa tgctcaacga
      541 gtacaaaggc cccccgacaa acagcgacag cagcaaaaac aagccatttt ccgcgaggat
      601 gaacgcaaaa ccgcaaagca aaaaatacgg catcacgcgc aaagcccctc taccatcttg
      661 ccctcgacgc ctcgccacca gccacggaac gaccaccatc cccaccgcgg cggccagcgc
      721 caagaccagt agccattgtg ccgatgccat cggatacaac caccccgagg gactcggagc
      781 cccccgtttt ccccggatcc aataaaaata gggacgatca tccgtcaccg gacgcacgtc
      841 gtaaggatac gcctccaaca gcgcctgtcg ttccgcccgg gaagctcgcc ccaaccggcg
      901 aaacatgacg tcgccaggcc gtttcacgat cgcgtcccca tcgatgaacg ggtcccatag
      961 cacctgaaag cctgcatccg cggcgaattg gcgcaaacgc tccacttctt gttgtgtata
     1021 atcttcccct tttttcacaa tcgcgttttc ccaaaacccc tcggatacca cacaaatccg
     1081 acgccccgga tcgtcgaccc cttgctcatc caatacctcc aagaacgtcg caaacatgcg
     1141 cagcgcaagc gtgggaggct ccaaatgcca atgcgtcaac gccaactgtc cgcgaggcga
     1201 aagcaccgac aaataatctt tcaccgcatc tttcgtatac aggtagtttt cgctcagcgc
     1261 attgcccgcc gtctgaatgg ccgtcagtgt atccacaccg ctcgccagca cgacatcgta
     1321 ggggcgctgc cctgcctggg aacgcacgaa atgccttgct tccgcttgaa aaatgcgaac
     1381 gtcggtgatg tcgtcggact tcaaaaaccg cgtatacgca agccgttccg ggtcttctgg
     1441 acgccccaac agcagcgaat acatgtccgg attgagttct gccgcatcta tctcatcgat
     1501 ttcataagct tttcctacaa gaaggtcgat gccgcccccg gggccgagca ccaagacgcg
     1561 ttcggtttcc ggacgcaaca cgtagggtat cgcccatagg gtccttccga gcattccttc
     1621 gcgcgtcggc ttttccccta aagcgaattg acgggtattg gcgctgccgt ccaccagcac
     1681 catgcgtccc cacagcggct cagaccagtc tttgcgggat aaaccatagc gaaacccgcc
     1741 aatatgagat tcgcccgtcg gactgacgtc gattcttgcg atcggattcc aataggtctg
     1801 aaccgcgcca ccgaaggtca tgaattctgc cttcaacggc gcgtgtttga aagacaccat
     1861 gtcgaagtgc aacgtggatt gcaaaacccg aggtacaagc agcacagccg agcacgaaag
     1921 cgccatggcc gtcgttccaa gcgcaagttc ccgcaaacgt cgtttcggct cgaggcttcg
     1981 aaggatgaga gcgccgacga atccgaggag ggaggcggcc acgacactac cgtagccgcc
     2041 tacgagcgag agccctaacg gtcccaagac ggcgcccatt ccagctccga aaagatccca
     2101 aaaataaacc cgcgtgggac gcatgccttt ggcgacgagc aaggcgctca cgcagacgcc
     2161 tgcaaaaaaa aacggaacag cgaaaagcag gaagtaaacg ggcagggaaa tgagcccaac
     2221 gagccccgga aatgcgggat cgagcggaag gacgagaaag agcgcaacga ctgcgggaac
     2281 ggaagcggca taccccaagg cccagggcca gagccgaagg gggtccgcct ctttttctgg
     2341 agaataccta tgagaaagca gatgcacgta caccccaccc acaccgaaac cgagaagggc
     2401 aatggagatc acggcgaacg tgtaatgatg ctccagtttg ttcgaaaaca gcttggtgat
     2461 caggacttcc aggtgcaggg tcgacgcagc caacaacgcc agcgcgagat acatcgccaa
     2521 gcggcgtcgt tgggcaaggt cgaaatagag cggggacatg cttcgcagtt cggagcctac
     2581 gagagggctt ggaggttggc tagactggat ttgcgggcgc agggaattgc catgccgaat
     2641 ggcagccgga agcaatgtcc ttctggcaat cggagatcac aggcgagaca tggcagatcc
     2701 caattttttt acggaaccaa gggaaataag aggattagtc aatgggcttg ggggcacgat
     2761 gtgtgcaaca attgaagccg atgaagccca aaaactgcca ttggtttgca ctgccgttcg
     2821 tggggatggt gatacttgcc agtgcttgtg gcgacggtga atcgtcaggc acggttgtat
     2881 cgaatgccgg cacgggaggg ggcaaaacct cggatgcggg acaagaaagc ggcgtggggg
     2941 ggtacggagg gacaggtggg gacgcggcaa cgggcggcac cggtgccagc gcaggaacgg
     3001 gaggctcggt cggtccaggg gggactggcg gtggtacatc gggctccggt ggagcggagg
     3061 tgggagggag cgcgggacag gcaggcagca gcggcggagc ggcaggcaac gcgggaagcg
     3121 agggcggtag tggaggctcg ccccccccgc cttgcaccac gcgcatcacc tacggttcct
     3181 cctggattca cggcgacaac catcccgatg acttcgacat cgcttctggc atcattacct
     3241 gggatggcag ttgccaggcc gatggcggca actcgtacgc gacgctttcc aacgggtgga
     3301 aaccctattt caaaggcaag ggcacctgcg ttatcgccct tgattatgaa gggtgtcaag
     3361 gcgtgcccgc gtcttgcgaa tcccgggtca cgtacgggcc caagtggatg cccgccccca
     3421 accatcccga ctatttcgac gatgtccaag gggctctcac ctggaatggg gcctgccaca
     3481 atgatggttc cgcctcctac gccacgcttt ccaacggatg gaaaccccat ttttcgggca
     3541 atagcgcctg cgacctggcg tttcggtaca cacaatgcgg cggactttat gtcaaccccg
     3601 tggtgggcaa ggattgtccc gacccagggg tcgttttcga tggaacccaa tatgtcatgg
     3661 catgcacggg cggtggcagt ggcgacgctt tcccgcttcg tcactccgac gacatggtcc
     3721 attggaaatg gaaggggtat attttcccta ccaaggacag accaagttgg gccgtaagcc
     3781 atttttgggc acccgaaatc cataaaattg gctccaaatt cgtcgcctat tttaccgcca
     3841 aacacaaaaa tggcgcgttg agcatcggcg cggccaccgc ctccgccgct ctaggccctt
     3901 acaaagatat cggcaaaccc ctgctcaaca acccttccat gggactcatc gatgccacgc
     3961 attatgaaga cccacaagga aaacactatt tgctgtggaa agaggatggg aatgcggtag
     4021 gcaaacccac tcccatctac ggtcaagagc ttgccagcaa cggtttgtcg ctcgtcggct
     4081 cacccgtaaa actcatcacc aacgacatgg cctgggaagg agccctcgtc gaaggcccct
     4141 ggttgataga tcacggcggc tactactact tattctatag cgcaaacggc tatgccagcg
     4201 cttcgtacgg agtcagtgtg gcacgggctt cctctcccct gggaccttat aaaaaagcgc
     4261 cggcccccct gctacgaacc gccggcgcct gggctggacc aggacatggc tccatcctaa
     4321 ccaccccccg tggcgaaacc tggcatatct atcattcctg gcaggccggc cacgtcggca
     4381 aagacccggg acgtgtcgtg ctgatcgacc gtgtgttctg ggatggggac tggcccacca
     4441 tgctcggcgc cccttcgagt gtctcactgc ctcctccctg acccctgccg gtatccgatt
     4501 aacttattga ttttacacat attttcttca gctcccccga ctcgttctcc ttgtgctaca
     4561 ccaaggcccc agcccccatc gattcctatt ccaccaaggt agactatcgt tgcatggcgg
     4621 aaaaggaggc ccggatgacc cccacaaccc attttgattt gaccccttgg ctatgcttgg
     4681 tcctcggcag tatcgcgctg accagcaccc ccctgggatg caaaagcgat catccccctt
     4741 ccccaaacaa taccgccgcc gggtccgcgc gcgcttccgc tcccaaggta tctaccagtt
     4801 ccgcgcgaaa accaaaagtg ccacccgcaa ccgctccagg gccatcgtgg aacccttccg
     4861 tgggcacgcg gcaagacatc accgatctgg cagccgatgc atatcatgtg gccatcggag
     4921 atgtcgacgg cgatgggcag ggggatttgg tatttgtcga cggccagcgg attcgcgtgg
     4981 tgactccttc cggtcgcctg gtgggggaag ccgaggtttc tgcgggaatt caggtgcttc
     5041 ggacgacgga tttggatggc gatggccgag ccgagatcgt cgcaggatgg ggacgctcgc
     5101 gccaccaccc cacggctacg acgcgggtga ccgttcatcg tctccagggc aatacccttc
     5161 gagaagaagt catcgttgca ccgacgacga ctcgcccaga cgtcgcgacg atcgtgccga
     5221 cggccaaaaa ctcgcttgcg attgcctatt ttgacagcaa gtacgaggtg aatctggttc
     5281 atgcccatcg acagcaggcc cgatggacgt tggaaacgct gggtcaggtg cgtatggcca
     5341 tgagcctggc ctggggggat gtggatgggg atgggtcttc ggacttggtc gtaggccgcg
     5401 tgtatggcga gacacaaggg ggggatggcg atgcgttcat cctgaaagcc aagggacagc
     5461 ggacagccct tcccacgctt cgaggggttc gcggtcttgc tgtcgcggat atggacgggg
     5521 acgggaaaag cgaaatctat cttggagatg gctggcatca ggattatggc aagcaagcga
     5581 aggggcttct ttcgcgaata acttgggcgg gcggagaata tcgaaaagaa gtcgttgaag
     5641 atacggctgg gcaatacacg ctatgggata ttttccccgc ggatatcgat ggcgatggga
     5701 gcaaggagtt gatcaccagg ggcagcgcgt atgttcgcgc gtatcggttt cggaaagggc
     5761 gctggcaagc ggttgctttt ggcggcatga gccgggggat tgccgtgggc cccttggatg
     5821 gcagcgctgg cgacgaggtg gtgctgcttg gcaaccgttg tgaggtggtg agtttactag
     5881 atgtgaattg ggccgatatc gagagatgaa cgtgcgtcgg tttctgcttc tggctgccac
     5941 gggacttggg gtatcgtcgg gttgcgattc cgtaggggta gcgaatcctt cgagacctgc
     6001 gtcgagcgtc cacgaagcct ttggcgttca acctggggcg gtgagggaag cgttcgatgg
     6061 gccgaaggca ggcgactcgc ggcagctcac gcagcaagcc cggctggtgg acgaaggcca
     6121 ggatccgcca tggcgttggg acgagcaggt tgcccgggta ttggattcga ccacccttgc
     6181 gcgggagctt ggatatcgac caccgcgagg tacggaagcg tacgcggcca tcatccagca
     6241 caatgaattg ggactgtcct acgccttctt cgattacgat aagggcgcgt tttctcaatc
     6301 tttttggccc gcttccacgg tcaaggtgct ggctgctctc gccgcgctgg attggctacg
     6361 gattcatgga ttcagcggcc aggccgaggt tcgctggaga aacggttttt ccgatcgggt
     6421 caatgccatc gtggaccggt cggtgcgtct cagttccaac gaagactacg atcgcacgtt
     6481 gcagatcgcg ggtttggatc acctgaatgg ccgttggctc acggcggaac ggggctttcc
     6541 gaataccgtc attcaggcgg cttatgccca ttccaacgtc aggaccccac ccagtttcac
     6601 acttacggaa ggggacaagc agtgggtcgt accctcgcgc acgacaggtc ccctcgacaa
     6661 gtgcagcggc gcaagggttc aatgcgccac tcttttcgag ctgacggaag cggtgcgtcg
     6721 ggtcgtgctt cgcgatttga ttccgcccca ggagcgtttc gagctatctt ccgaggattc
     6781 gaacgcactg caagaagctc tatgccaggc aaaaccgtcg ttttttgccc gcggggtgcg
     6841 cgccgttctt ggaccggacg ctcgcatttg ccacaagccc ggctgggttc ccggcaagga
     6901 ttacttggat cacggggtca tggaagccgc ggatggtcgt cgcttcttgc tggcggcggc
     6961 gatgcccgat cctggggatg ggtccgcgca agggcggctt ccggtgttgg ccgagaaaat
     7021 ccttcgggtt atcactcgct tggaaatgga gcccttttat ttgcagccga cggcaggcgc
     7081 ggagatccgt gtgaaaaagg aagctggcgg gcttcgtgtg caaggcgagg ggtttgacga
     7141 ggtgcaggta tatatcgatg gcacgggagt ggatcgggtg cggcgtgacg aagagacatt
     7201 ttttgccgag gtgacgacgg agtcgtggca ggataagctc atgacggtgc gcgggtatcg
     7261 ccagggcgcc atcgtagggg ttcggaatct ggtattgccc tgacaaatcc ccttatattt
     7321 tcctgaatta gaaagggata cgagctgatg attcttgggt cttgattttg gctcggtcct
     7381 tggcaccccc cttggagcat ccccaacagt tacgctgggc tgttaaacat tggattggcg
     7441 ttatgcggtg aacagtatgg gatgactgtg accgtttttg tgtaccgtgc gccgtgaccg
     7501 tgaccgtgac cgtgaccgtt ttcgtgcgcc gcgggccgtg accgtgacca agcgatggca
     7561 gatagttgtt tcgaactgtc gtagggtgaa ggaaaggctt ggggaaaatg aggggatagc
     7621 tcagggtttt gccctgatcg tctcgtcatg agccaaaggg ggacaggcga tgggcagggt
     7681 tggggggagc cgagcgccat cgaaagaagg ggggaaatca gataatagct tttatttcaa
     7741 taggttatgc cacatccgtg ggctgggcgg cccatggatc ggctaccaat tcacgcacgg
     7801 actccgtccc tgatagtgcg gtgagggtat ggatttgccg tttcgccacg atgcgcgatt
     7861 caccgttgtg tttgagtttc aggaaggtcc catccaccca cacgagttct ccctggacgc
     7921 gggttccgtc gtgaagggtg gcttcgacca ccaccggtcc ggaaggtttg gcgagttgcg
     7981 tggccgattc gacgagcacc tgatccaaac tgtcgaaagc atccgttatc tttgatattt
     8041 tttgcagggc atgctcgaaa tcttcgcgag ccaccgatat tttttctcct tgcgcgcggc
     8101 atcgtcgcac cgccagaaga gcggcttcgt tggcaaggtt ttccagcatg gcgccgttta
     8161 caccctcgca acggtcggcg agtttttgca gggagacatc gtcggccagg ggcatggggg
     8221 cggtatgaat tcgtaagatc gcgagccgtg tctcggcgtc gggcaacggg atacggattc
     8281 gacgatcgag tcttccgggt cgaaccagag cctggtcgag gatatcgggg cgattggtgg
     8341 cggcgatgac gaccacgcga tcgtcggttt cgaacccatc catgcatgac agcaattgat
     8401 tgagggtttg ctcacgttcg tcgtgggcgc tgccgacgcc ggaacctctg cgacggccca
     8461 cggcatcgag ttcatcgatg aagatg
//
LOCUS       DAOSOR010000205         8483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8302_length_8483_cov_4.905553, whole genome shotgun sequence.
ACCESSION   DAOSOR010000205 DAOSOR010000000
VERSION     DAOSOR010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8483
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8302_length_8483_cov_4.905553"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PK710_12265"
     CDS             complement(<1..728)
                     /locus_tag="PK710_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT10542.1"
                     /translation="MSKYARTIVIWVVLVALFLVVWKFLSTEEKHLEFREFTVFLQDV
                     VADRIASVRVLGNRLIVTPEEGLVYETVGVLDSETMEDLTRQGASIHYGQEPQETSVS
                     TSFLYWAGAVAVLVVVALVLRRMRGSGGGLITLGKSRARILPENRTATFAQVGGANEA
                     KKDLQDIIDFLRSPATWKSAGIRLPRGVLLEGPPGCGKTLLARAVAGEAKVPFFFVSA
                     SEFVEMFVGVGAARVRDTFETASK"
     gene            1029..2087
                     /locus_tag="PK710_12270"
     CDS             1029..2087
                     /locus_tag="PK710_12270"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT10543.1"
                     /translation="MTRLTLYSLAIELTWACNLRCSHCYNPLHISKSLDSSTDRLLER
                     IDKIFDAVQVGHITLTGGEPLSYPRLFEVLAALKRHGVPCQMISNGTLLDAAYAKRLV
                     EAKLRGIQISLHGPDAQTHEDYTRTQGSFERAVAGVQAANQAGLHVTGCIVVTRRNAH
                     WVGATLGLWQKLGVQRVALSRFSPSGLASQHVARLLPSIAELMTAFEQATPYARGGMR
                     LFCTMPIPPCAMDTKLYAPIRFGSCAVGTTKQEFALSPGGALRFCTLHTQAIGGVEDI
                     LDVEDVSSLFHHEQVVTYREGAPSFCRGCYYERSCGGGCGAASMSVYGHREQPDPLLW
                     QHVDDAFREGLDRQREGG"
     gene            complement(2741..4378)
                     /locus_tag="PK710_12275"
     CDS             complement(2741..4378)
                     /locus_tag="PK710_12275"
                     /inference="COORDINATES: protein motif:HMM:NF041328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_6577-like cysteine-rich protein"
                     /protein_id="HOT10544.1"
                     /translation="MYRRKIGVTLQNCLCFIALIFMVSGCPKEEGCPEGQEMCAGKCV
                     TLATDPYHCGSCGKSCGPGMACSSGVCGASCQPDMSVCMGKCVDYQTDSLNCGACGHQ
                     CESGTRCSSGICGPTCQPHMSLCMGKCVDYQTDSLNCGACGNQCGSGMTCSYGICGVS
                     CPLDLSLCGNKCVNYQTDIHNCGYCGYECGPGLQCASGNCVPTCAEPLKQCGDRCVDI
                     TNDPEHCGSCNNKCGSGQICAGLSGCQDLSCPQLCPPDQTLCSGHCVDTMGDVLHCGG
                     CGNACGAEEACMAGVCTAGTFRIVSLDTQCRIVDDTSKLDAPRGPIAFGEGLVFYSGA
                     SRVLSYDFADYWAEIMSQEAIPGLTSDLASGKLWALSTNQGWAVAAGATITKIIPVIN
                     EMPLVAGTPIPLSESFQIPAGSGIFAGFGHVAVHTGTELIVIDTANGEVTRLASSALQ
                     AAPCQGPFYSGIIEMHADGMRLTYVQASTSAIVRTRPGDGATQVVLQGIDFADMCGIT
                     VDLSRSRWCFHLAQPSQEFDTQPGQSATYCCDAVFAM"
     gene            complement(4847..6247)
                     /locus_tag="PK710_12280"
     CDS             complement(4847..6247)
                     /locus_tag="PK710_12280"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOT10545.1"
                     /translation="MPKPKDAQTQEHDLENLLADISHENTGAERWWSKSELIEFIGKS
                     LNAPADSAVARIRAAAKTNGWIGKVSVHCVGQRRGTRYAFGRKPENAPVAHEGKRMPA
                     GSTSEELGGIRDFPVPANPAEAGSVQVFHGDNLEIIRGFDDQSFDLIYIDPPFNTGRP
                     QSRTRIRTERDEKGDRVGFQGKRYRTTVVGVSGYADVFDDYMAFLVPRLHEAYRLLKP
                     TGSLFLHLDYREVHYAKVALDQIFGRDSFINEIIWAYDYGARATHRWSPKHDNILWYA
                     KDPHQYCFNYEEIDRIPYMAPSLVGEEKAARGKTPTDTWWHTIVSPNGKEKTGYPTQK
                     PLGILSRIVRIHSKKGDKLLDFFAGSGSFGQAAASHGRDVTLVDNNPEAIEVMRRRFA
                     NYLCTFPRDDAGLESSAGLPRTPPTPSLPFGTDTNSGHPEVEKAAPKKAPSRSGSRKA
                     ITRKSTGTGTKQGSLF"
     gene            6588..>8483
                     /gene="uvrA"
                     /locus_tag="PK710_12285"
     CDS             6588..>8483
                     /gene="uvrA"
                     /locus_tag="PK710_12285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOT10546.1"
                     /translation="MEPEWIEIDGARQHNLRIEHLAIPKKKLVVFTGVSGSGKTSLAF
                     DTLYAEGQRRYVESLSSYARQFLGVMEKPAFEKLRGLSPTIAIEQKSASNNPRSTVGT
                     ITEIYDYLRVLYARIGIQHCPRCEEVVAPLTIEQAVRELREIDRDVWLLATIVENRKG
                     EHREVLEGLRQRGFLRVLVDGTLERLDSLVTLTKARKHTVQLVVDRVNPHKVSVSRLT
                     DSVETAMREGQGSLLAVCPHGRMPTVRLSQHRACGSCGIGLPDLSPQSFSFNSPLGMC
                     PTCHGMGRRWEMDARLVVPNESLSIRQGAIEPWASIMERGSGWSYEIFSGLEKAFGID
                     FDRPWKDLSKKQRDIVLRGTQGRRIEIEHRHRSGSGTWSIEFEGVLNTMLRRYSETRS
                     DAMREYYQKYFSDAPCSDCEGRRLRKESLSVRVGGRSIAEVVGMSVDRAREHFEALSL
                     HGQDLIVAREILREIKGRLDFLRSVGLGYLSLDRAGPSLSGGEAQRIRLASQLGSELS
                     GVMYVLDEPSIGLHARDNLRLIRTLERLRDTGNTVLVVEHDAQMMLHADYVVDFGPGA
                     GEQGGQVVFSGTPEQLRASDTSTAQYLSGKKQIALPWKRRKARGKLVVRGARANNLKG
                     IDVAFPLG"
BASE COUNT         1751 a         2195 c         2622 g         1915 t
ORIGIN      
        1 ttttttgagg ccgtttcgaa ggtatcgcgc actctggcgg cacctacgcc cacgaacatt
       61 tcgacgaatt cggaagcgga gacgaagaag aaggggacct tggcttcgcc ggcgacggcg
      121 cgagcgagca aggttttgcc gcagccgggg gggccttcga gcaggacacc tcggggcaat
      181 cgaatgcccg cgcttttcca tgtggcgggg gaacgaagaa aatcgatgat atcctggagg
      241 tcttttttcg cctcgttggc gccgccgacc tgggcaaacg tggccgtacg attctcgggc
      301 aggatgcgcg cacgactttt tccaagggtg atcagcccac ccccactgcc ccgcatgcgc
      361 cgcaacacga gggctaccac gaccaaaacg gcgacagcgc ccgcccaata gaggaatgag
      421 gtggagacac tcgtttcctg gggctcctgc ccgtagtgga tcgaagcgcc ttgccgtgtg
      481 aggtcttcca tggtttcgga atccaacacg ccaacggttt cgtagaccaa accttcttcg
      541 ggggtgacga tcaatcggtt accgagcacg cgaacgctgg cgatacggtc tgccaccacg
      601 tcctgcaaaa aaactgtaaa ctcacgaaat tccaaatgtt tttcttccgt ggacaggaat
      661 ttccaaacca ccagaaatag cgccacgagc actacccaga tgacgatggt gcgagcatac
      721 ttggacatat cggcaaaata gcagagtggt gcaggcagga gaaagcgatt gatgattcgg
      781 aaagaggcgt cggcggaaaa agggataaag cggttcgaat cacggcgctt cgatccggtc
      841 catgaggatg catgcgggag atggcctgtg attattgggc cgagttctgg cctagggcga
      901 aggccacggg ctattttggg tcatcgagga tcccaacacg cggaagactc gagaatatcg
      961 cctattttca tggctgcgat ttcgagcttt gatggttttg cgaatcggac ggtcgaatca
     1021 aggtatccat gacgcgtctt accctctaca gcctggcgat tgaactcacc tgggcgtgca
     1081 acctgcgctg ttcgcactgc tacaatccgt tgcatatatc gaaatcgttg gactcgtcga
     1141 cggatcggct cctcgagcgt atcgacaaga tttttgatgc ggtgcaggtg ggccatatca
     1201 cgttgacggg cggcgagccc ctcagttatc cgcggctttt cgaagttctc gccgcgctca
     1261 aacgtcatgg ggtgccttgt cagatgattt cgaacggaac gttgttggac gcggcgtatg
     1321 cgaaacgtct tgtcgaggcg aagctgcgag gcattcagat ctcgctgcac ggaccggatg
     1381 cgcaaaccca tgaagattac acgcgaacac aagggtcttt cgaacgagcg gtcgcggggg
     1441 ttcaggcggc gaaccaggcg ggactgcatg tcaccggatg catcgtggtc acgcgtcgca
     1501 atgcgcattg ggtgggcgcg acccttgggt tatggcagaa gcttggggtt caaagggtgg
     1561 cgttgagccg attcagtccg tcggggctgg catcccagca tgttgcgcgg ttgttgccat
     1621 cgattgctga gctgatgacg gcgttcgagc aagcgacgcc gtatgctcgc ggcgggatgc
     1681 gattgttttg cacgatgccg attccgccgt gcgcgatgga tacgaagctt tatgcgccga
     1741 ttcgattcgg aagttgcgcg gtgggaacca cgaagcagga atttgcgttg agtccaggtg
     1801 gggctttgcg attttgcacg ttgcacacgc aggcgattgg gggggtggaa gatattctgg
     1861 atgtcgagga tgtgtcttcg ctgtttcatc acgagcaggt ggtgacctat cgggaagggg
     1921 cgccttcctt ttgccgggga tgctattacg aaaggagttg tggggggggc tgcggggcgg
     1981 catccatgtc ggtatacggt catcgagagc agccggatcc attgttgtgg cagcatgtgg
     2041 acgatgcgtt tcgagaaggt ctcgaccggc agcgcgaagg aggctgagac gagggcgggg
     2101 ttcgatggcc cgttagcggg ttacggtgag ggggcgagag aaaaaagcgg ataacaggct
     2161 tcagcgtcga gaggccctct aagccatccc ctcattttcc ccgagccttt ccttcacggt
     2221 cacggcccgc ggcgcacgaa aacggtcacg gtcacggtca cggcccacgg cgcacaaaaa
     2281 cggtcacggt catcccatat tgttcaccgc agaacaccaa gccagtgttc agtagcccag
     2341 ggcaactgtt caattcagga aaaatgaggg gatctgtcag gagcccctca cccgctgaaa
     2401 cctatgcaac tatttggaat cattatacaa ttttctattc ctgcaacatg gccccttgca
     2461 tggtgacgat ggaaaatggc gacgatgtcg agcgatgctt tggggcgcgt tcaatggtga
     2521 tatgggcttt gtcacgaggg gaatgtcagg atggtggtgg gaagaaggca gcaggaggcg
     2581 tggaggggca gagcttatcg gataagccag aaacaggtgc caggatgacg ggcgcggcaa
     2641 gggatgttgc gtcaggggct gatggggatc gtgttgagga tttcctggcc ggatggcttg
     2701 ctacgttggc gcggctttgc tgccggtggc tcaggttccc ttacatcgca aatacggcat
     2761 cgcagcagta ggtagcggac tggccgggct gggtgtcgaa ttcctgggaa ggctgggcca
     2821 ggtggaagca ccatcggctt ctggacaggt cgacggtgat gccgcacata tcggcgaagt
     2881 cgattccttg caggaccacc tgggtggcac cgtctccggg gcgcgtgcgg acgatagcgc
     2941 tggtgctagc ctggacgtac gtcagcctca tgccgtcagc gtgcatttca atgataccgg
     3001 agtaaaaggg gccctggcag ggggcggcct gaagcgctga gctggcgagg cgggtgactt
     3061 cgccattggc cgtatcgatg acgatcaatt cggtgccggt atggacggcc acgtgtccga
     3121 agccggcaaa aatacctgac cccgctggga tttggaagct ttccgacaac ggaatcggtg
     3181 ttcccgccac gaggggcatc tcattgataa cagggataat tttcgtgatg gtagcgccag
     3241 cggccactgc ccatccctga ttggtggaaa gggcccataa cttcccgctt gcaaggtccg
     3301 aggtgagacc gggtatggct tcctggctca tgatctcggc ccaataatcc gcgaaatcat
     3361 agctcagcac tcgggaagcc ccggagtaga agaccaatcc ttccccaaag gcgatggggc
     3421 ccctgggtgc gtcaagcttg gaggtatcgt ccacaatccg gcattgcgta tccagggaga
     3481 cgatgcggaa cgttccggcg gtacagacgc ccgccataca ggcctcttcg gcaccgcaag
     3541 cgttgccgca cccgccgcaa tgcaggacgt cgcccatggt gtcgacgcag tggccgctgc
     3601 aaagcgtctg gtctggggga catagttggg gacagcttaa atcctggcag ccggaaagac
     3661 cggcacatat ctggccgcta ccgcatttat tgttgcaact tccgcagtgt tcggggtcgt
     3721 tggtgatgtc cacgcatcga tcaccgcatt gctttagcgg ctcagcgcag gtgggcacac
     3781 agtttccgct ggcgcactgc agccccggac cgcattcgta gccgcaatat ccgcagttgt
     3841 ggatgtcggt ctgataattg acacatttgt ttccgcacag actcaggtcc agcgggcaac
     3901 tcactccaca tatcccgtag ctgcacgtca tccccgaccc gcattggttg ccgcatgcgc
     3961 cgcagttcag gctgtcggtc tgatagtcca cgcacttgcc catgcacaga ctcatgtgtg
     4021 gctggcaagt cggcccgcat atcccgctgc tgcacctcgt ccccgactcg cattggtggc
     4081 cgcatgcgcc acagttcagg ctgtcagtct gataatccac gcacttgccc atgcatacac
     4141 tcatgtccgg ctgacaactc gccccacata ccccgctgct gcacgccatc cccgggccgc
     4201 atgacttgcc gcaggaacca caatgatacg gatcggtggc caacgtcacg catttccctg
     4261 cgcacatctc ctgcccttca gggcaccctt cctccttcgg gcaacctgat accatgaaga
     4321 tcagggcaat aaaacataag caattttgaa gagttacccc aattttgcga cgatacatca
     4381 tgcctctctc ctgttcccgt ccgtgatccg attcacgcca ccatcaccat acctagaacc
     4441 tgatcctcat tttagcctga ctttcctgtc aatgcaagcc ttcccctagg ccgagacgat
     4501 aagcatattc aataatattc gttagatggt aaatggtatg gcctcacctt ttcgacacac
     4561 ttatttgtgc gagtttcacc cacttttgcg tcatggaaaa gagctttggt ccgcggtcga
     4621 ctctgccaaa accgagaaaa taactcggag acaatccttt gaaggtccga aatccaagtt
     4681 caggttgccg ttgctgccaa agtcgaaatc aagatcgagg ataactctct gattgaatct
     4741 ctaccccatg ccagaaatca cgagggctct tgtcgacgat aggggcatgg tcgcgattcg
     4801 gccacgatga aaagcagcgt taggaggcat tattatacgt aacctatcag aataagcttc
     4861 cttgttttgt ccccgtccct gtcgatttgc gggtgattgc tttcctgcta ccggaacgac
     4921 tgggcgcttt tttgggagcg gctttttcga cctcaggatg gcctgaattc gtgtcggtgc
     4981 cgaaaggcaa agatggagtc ggaggagtcc ttggcagccc ggcggaagat tcgagaccgg
     5041 catcgtcccg aggaaaagtg catagatagt ttgcgaatct cctgcgcatg acctcgatgg
     5101 cttccgggtt attgtccacc agggtaacgt ctcgtccatg gcttgccgcg gcctgcccaa
     5161 acgaaccgct gccggcaaaa aaatcgagga gtttatcacc ttttttcgaa tggatacgaa
     5221 cgatccgaga caggattccc agaggtttct gcgtgggata gccggttttc tccttgccgt
     5281 tgggactgac aatggtgtgc caccaggtat cggtcggagt cttgccccga gccgcttttt
     5341 cctcgcccac gagagacgga gccatgtacg gaatacggtc gatttcctcg tagttgaaac
     5401 agtattggtg aggatctttg gcgtaccaga gaatgttgtc gtgtttgggg gaccaccgat
     5461 gggttgcccg tgcgccgtag tcataagccc agatgatttc gttgatgaag ctgtcgcggc
     5521 caaaaatctg gtccagggcg accttggcat aatgaacttc gcgataatcc aggtgcagaa
     5581 ataggctccc cgtgggcttg aggagacggt aggcctcatg aaggcgggga acgagaaacg
     5641 ccatgtagtc gtcgaaaaca tcagcgtatc cgctcacccc caccacggtg gttcggtatc
     5701 gttttccttg aaaaccgact cgatcgccct tttcgtcgcg ctctgttcga atacgggtac
     5761 gcgactgtgg acgtccggtg ttgaagggcg gatcgatgta gatcagatcg aaagattgat
     5821 cgtcgaaacc gcggataatt tcgagattgt caccatggaa tacttgcacg cttcccgctt
     5881 cggcggggtt ggcaggaaca gggaaatccc ggatgcctcc cagctcctca gaagtgctgc
     5941 ccgcgggcat gcgttttccc tcgtgcgcta caggcgcgtt ttctggcttt cgtccgaagg
     6001 catagcgagt gcctcgtcgt tgccccacgc aatggacgga aacctttccg atccatccat
     6061 ttgttttggc cgcggcacga atccgcgcaa cggccgagtc tgccggggca ttcaatgatt
     6121 tgccaataaa ttcaataagt tctgatttac tccaccagcg ttctgcgccg gtattctcat
     6181 ggctgatgtc ggcgagcagg ttttccaggt cgtgttcttg ggtctgagcg tccttgggtt
     6241 tcggcatggg ctgctcctgt ccacggattg cggttgaaac gaagatgtac cgtcatcaca
     6301 ccgcggggat tctccagcca tcctttcaga aatccgattt gcttttcgtt atcgcgacca
     6361 cggcgttttt cgcaaccctt gagccgtacc ccgtagcgtg aatggtcgag gtgtcaggtc
     6421 attcgaacgc gattcgcgag tcaacgcaac acgggggcga tgggtttcgg gagcctgtcg
     6481 atcggtatcc acgaggtcca tcggcgtgcg gcggtgccag cggcttgttc tcgactgaaa
     6541 aacggaatag gctgaacgtt ctttcagtat cttttggagt catgtggatg gaaccagagt
     6601 ggatcgagat tgatggggct cggcagcata acttgcgcat cgagcatctt gccattccca
     6661 agaaaaagct cgtggtattc acgggggtga gtggttcggg caagacctcc ctggccttcg
     6721 atacgctcta tgcggagggc cagaggcgtt atgtggagag tttgtcctcc tacgcgcgtc
     6781 aattccttgg ggtgatggaa aagccggcgt tcgagaagtt gcgggggctg agtcccacga
     6841 tcgccatcga gcagaagtcg gcatcgaaca atccgcgttc gacggtgggg acgatcaccg
     6901 agatttatga ttatttgcgt gtgttatatg cccggattgg gatccagcat tgtccgcgat
     6961 gcgaggaggt ggtggcaccg ttgacgatcg agcaagcggt tcgcgagctt cgggaaatcg
     7021 atcgggacgt atggctgctc gcgaccatcg tggaaaaccg aaaaggcgag catcgcgagg
     7081 tgttggaggg gttacgtcaa cgaggttttt tgcgcgtgct cgtggacgga acgttggagc
     7141 gtctggattc gctcgtgacg ctgacgaaag cgcgcaagca tacggtgcaa ttggtggtgg
     7201 atcgggtgaa tccccataag gtttcggtgt cacgtcttac ggattccgtg gagacggcga
     7261 tgcgcgaggg gcagggttcg ctattggcgg tctgtcccca tggccggatg cctaccgttc
     7321 gtttgagcca gcatcgggcg tgtggctctt gcggcattgg acttcccgac ctttctcccc
     7381 agagtttttc cttcaacagt ccgttgggaa tgtgtcccac gtgtcatggg atggggcgtc
     7441 gatgggagat ggacgccagg ttggtggtgc cgaatgaatc gttatcgatt cgacaagggg
     7501 cgattgagcc ttgggcatcg atcatggagc gagggtcagg gtggagttat gagattttct
     7561 cgggattgga gaaggctttt gggatcgatt tcgatcgtcc ctggaaggat ttatcgaaga
     7621 aacaacggga catcgtgtta cgagggaccc aaggtcgtcg catcgagatc gagcatcggc
     7681 atcgctcggg ttctggcacg tggtcgatcg agttcgaggg ggtattgaac acgatgcttc
     7741 gtcgatattc ggaaacgcgt tccgatgcga tgcgcgagta ttatcagaag tatttttcgg
     7801 atgcgccatg ctcggattgt gaggggcggc gtttgcggaa ggaatctctt tccgtgcgcg
     7861 tgggcggtcg aagcattgcc gaggtagttg ggatgtcggt ggatcgggcg cgcgagcact
     7921 tcgaagcact atccctacac ggacaggacc tcatcgtggc acgcgagatt ctccgggaaa
     7981 tcaagggtag attggatttt ttgcgttccg tgggtctcgg gtatctttcg ttggatcggg
     8041 ctggaccatc gttgtcggga ggggaagcgc agcggattcg attggccagt cagcttggaa
     8101 gcgaattgtc tggtgtgatg tacgttctcg atgagccatc gattgggttg cacgcccggg
     8161 acaatcttcg gttgatccgc acgttggagc gcttgcggga tacgggcaac acggtattgg
     8221 tggtggagca tgatgcgcag atgatgcttc acgccgatta tgtggtggat ttcggtcctg
     8281 gggcgggcga gcagggcgga caggttgtgt tttccggaac gcccgagcaa ttgcgtgcca
     8341 gcgacacgtc gacggctcag tatttgtcag gcaaaaagca aatagcccta ccttggaagc
     8401 ggaggaaggc acgaggcaag ctggtggtgc gcggggctcg agccaacaac ttgaagggca
     8461 tcgatgtggc gttcccattg ggc
//
LOCUS       DAOSOR010000206         8472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8318_length_8472_cov_4.400261, whole genome shotgun sequence.
ACCESSION   DAOSOR010000206 DAOSOR010000000
VERSION     DAOSOR010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8472
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8318_length_8472_cov_4.400261"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PK710_12290"
     CDS             complement(<1..604)
                     /locus_tag="PK710_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10547.1"
                     /translation="MKCMHCSWVIASIVLVMGCGGGQSAANAPAEAPSAAPTQQPKPA
                     EPEAKPEPEKFDVDFVEAKPSAAVDKPPRVTIDVPGFNQFLPASLVANTRIRFKVQGF
                     SDAPEGSYLQFILDNKPFRPVTDPNEKIMLTDLAGAEGLADGEHIIAAFMSYPTHEAI
                     KSEKGIAVRRFYVGKRSDGGWDSNRSPLLLLASPHGTVAED"
     gene            complement(726..1742)
                     /gene="cydB"
                     /locus_tag="PK710_12295"
     CDS             complement(726..1742)
                     /gene="cydB"
                     /locus_tag="PK710_12295"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOT10548.1"
                     /translation="MPDLPTTWFVLVGVLLTGYAILDGFDLGVGAWHLFTKDDKERRV
                     MLNAIGPVWDGNEVWLVTGGGALFAAFPEVYATAFSGFYEALMLLLFCLIFRAVSIEF
                     RGKQPMLWWRRLWDYGFSIGSLVAALLLGVAVGNVAWGLPLDERHEYAGSFVGMLHPY
                     ALLVGVTTVALFMMHGSLYLYVKSDGSLQDKARRWARAALIGFVVCFVLTTAATFWAV
                     PHMVDRFTTYPALFVLPVLIVLMLGNIPRELRLGRGFRAFLSSCIAMAALAGIFGLGM
                     YPNLIFANPVEHSLHIHNAASSTKTLTVMLTIAAIGMPLVLAYTSFIYWVFRGKVKID
                     ATSY"
     gene            complement(1742..3109)
                     /locus_tag="PK710_12300"
     CDS             complement(1742..3109)
                     /locus_tag="PK710_12300"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOT10549.1"
                     /translation="MDVDLLARLQFAFTLMVHYVFPPLSIGLGVLLVAMEATYVKTRD
                     PDYEQMTKFWVRVFGLIFAVGVATGIVMEFQFGTNWSTYSRFVGDVFGSPLAAEGIFA
                     FFLESGFLALLLFGWNRVKPGVHLFATAMVALGSHLSAVWIVIANSWQQTPAGHHIVG
                     EGSQARAEIVDFWAVVFNPSSVDRISHTIMGCWQAGAWLVLSVSAYYLLRGRHEKIAK
                     KSMQLALVLALVSSLGQLATGHTSAEGVGANQPAKLAAFEGHYEASAPADLYLFGWVD
                     EKNEKVIGIGIPGMLSMMLHRDFKAPVTGLRAFAPEDRPPVQIVFQSYHAMVGIGMIL
                     IGLAAGGLMLLRGGRLYRMRWLLWTFVAAVLLPQLANQLGWMSAEVGRQPWIVYGLLR
                     RVDGLSRVVNANQVLWSIIMFALIYALLMAVFLFLLDKKIKHGPGPDEEATVADGMLT
                     EGRDA"
     gene            3331..4842
                     /locus_tag="PK710_12305"
     CDS             3331..4842
                     /locus_tag="PK710_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IG family 5'-nucleotidase"
                     /protein_id="HOT10550.1"
                     /translation="MSSSMLKPEQLALPFPEIQTAPRTIIARVHRIFANQSMKMGDVE
                     WIGFDMDHTIAMYDPSALDSLCIHSMIDRLRQQGFPHAPTNVDPSFCIRGLVIDKRLG
                     HILKLDRYRVVRKGFHGLRELSPDQLAKTYEARRIRVGTPQFFGIDTLYARSEASLFA
                     AWIQSLEQQRHSIDPSHLFDQIRTCADGLFRDGTIPRLITDDLRRFIRKDPQLASTFH
                     KLRSGGKKLFLLTNSPWPFTHAVMDYLLGSEMPEYPTWRHYFDIVITSANKPQFFLER
                     QPLIEQDGDSKRPVVLRASTSFERGKSYEGGNLSDFTRLLGVPGDRILYVGDHIYGDI
                     LRSKKEASWRTGLVLHELDDELRAHEASLPDIQRLQDLTSQREELEDELRVHQTLFKD
                     QSRQLESVRATRDIAMTSAVEVQRQLEKRTVERVRGLLQSNESETRDIEARIEQRFHP
                     TWGPLLKEGTELSSFGQQVEAYACVYTSRVSNLLFYSPIQHFRSPRDLMPHEF"
     gene            complement(6485..6586)
                     /locus_tag="PK710_12310"
                     /pseudo
     CDS             complement(6485..6586)
                     /locus_tag="PK710_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263577181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
     gene            complement(6896..7189)
                     /locus_tag="PK710_12315"
     CDS             complement(6896..7189)
                     /locus_tag="PK710_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10551.1"
                     /translation="MGRPNFSICGGFAALGGGGDPREKEGRTGGGGDVRTGGGTPEPG
                     GGGTVVRAGGGLLARSSSCAGGAINVDSSSPTVLVGGGRTGGSRLDRVIFGKE"
     gene            7206..8147
                     /locus_tag="PK710_12320"
     CDS             7206..8147
                     /locus_tag="PK710_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10552.1"
                     /translation="MSQLPGIASTKNITVRPTGQPPLAPTSDQPLPKSVGLKVLGMWI
                     GGAAVIGLLVSAIGAWWFLPKPQTHHHPIASATVASPTRTRSTPSGPASTESETTPSA
                     LSLPSLAERAAQGDPDAIDQIERRPAPNRTFNESLAWARGKSVFKHRQLELIAKELRA
                     DLPLSADRRTLAKLKPFTEDPETATEALAILASLRTPLAVDMLYDVWVRRRDGTIAFE
                     LAKSLLYTPEIREAASDALAVVLDLRAASTCEQVAELLPRVQQRADRRALPMLGKLTS
                     RYGCGRLGGHDCYPCLRGSNAVNDAIAAVGNRPAPDI"
     gene            complement(8192..>8472)
                     /locus_tag="PK710_12325"
     CDS             complement(8192..>8472)
                     /locus_tag="PK710_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HOT10553.1"
                     /translation="GLPRSLSNKGRMLVRGTTAPIVGNVSMDMTMIDMTDVPGASLRD
                     EVAVLGPQQGPLGSDCITADEIANHADTISWEILTGISRRVPRFFREP"
BASE COUNT         2062 a         2776 c         1964 g         1670 t
ORIGIN      
        1 gatcctctgc caccgtccca tgagggcttg caaggagcag caaaggcgat cggttactat
       61 cccatccccc gtcgcttcgc ttgcccacat aaaaccttcg tacagcaatg cctttctcag
      121 acttgatcgc ttcgtgagtc ggatagctca tgaacgcggc aatgatatgc tcaccatccg
      181 caaggccctc cgctcccgca agatccgtaa gcatgatctt ctcattcgga tccgtcacgg
      241 ggcgaaatgg cttgttatca agaatgaatt gtaaatacga gccttccgga gcatccgaaa
      301 agccctgtac tttgaagcga attcgcgtgt tcgccacaag agacgctgga agaaattgat
      361 tgaagcccgg cacgtcgatc gtaacccgag gaggcttatc cacggcagcc gatggtttgg
      421 cctccacaaa atcgacatca aatttttcgg gctctggctt cgcctcgggc tcggccggtt
      481 taggctgttg cgtaggagca gccgagggag cctccgcggg ggcattggcc gcggattggc
      541 cacctccgca ccccatcacc agcacgatag acgcaatgac ccaagaacaa tgcatgcact
      601 tcatcaatca acctttcgat gaaccaatcg ctcggcgatg cttagagaag gttacccgcc
      661 aggtcaacgg gtgtccgccg actcccccaa ggcaagtatt ccacggtggg agaattgacg
      721 atgcttcagt agctcgtggc gtcgattttt actttgcctc gaaacaccca gtagatgaac
      781 gaggtatacg ccaacacgag cggcatcccg atggcagcaa tggtgagcat caccgtcaaa
      841 gttttcgtag aggaagcggc gttgtgaata tgcagcgagt gctcgacggg attcgcaaaa
      901 atcaggttcg gatacatgcc gagcccaaaa attcctgcca acgccgccat ggcgatacac
      961 gatgataaaa acgcccgaaa cccgcgtcca aggcgaagtt cccgtggaat attccccagc
     1021 atcagcacaa tgagcacagg caatacaaaa agcgcgggat acgtggtgaa cctgtcgacc
     1081 atatgcggca cagcccagaa tgtggctgcc gtggtgagca cgaaacagac gacgaaacct
     1141 atcaaagccg cccgcgccca gcgtcgcgcc ttgtcctgta atgaaccgtc gctttttacg
     1201 taaaggtaca aacttccgtg catcatgaac agcgccacgg tcgtcacccc taccagcagc
     1261 gcgtaaggat gcagcattcc cacgaaagat ccggcatatt catgccgctc atccaacggc
     1321 aacccccagg ccacattgcc cacggccacc cccaacaaca gcgccgccac gagactgcct
     1381 atcgaaaaac cataatccca taaccggcgc caccacagca tgggttgttt gccgcggaac
     1441 tcgatcgaaa ccgcacgaaa gatcaaacaa aacaaaagca gcatcagcgc ttcgtaaaat
     1501 cccgaaaaag ccgtggcata gacttctgga aacgcggcaa ataacgctcc gcctcccgtc
     1561 accaaccata cctcgttgcc atcccacaca gggccaatgg cgttgagcat cacccgacgc
     1621 tccttgtcgt ctttggtgaa aagatgccac gctcctaccc ccaagtcgaa accatcgagg
     1681 atcgcatagc ccgtaagcag tacccccacc aaaacaaacc atgtcgtcgg tagatcgggc
     1741 atcacgcatc cctcccttcc gtcagcatcc catcggccac cgtcgcttct tcatcgggac
     1801 caggcccatg tttgattttt ttgtccaaca aaaacaagaa gacggccatc aaaagcgcat
     1861 agatcaaagc gaacatgatg atggaccata gaacttggtt ggcgttcacc acccgactca
     1921 gaccatcgac tcttctgagc aacccataca cgatccatgg ttgccgtccg acttccgcgg
     1981 acatccaacc gagttgattc gcgagttggg gaagcagcac ggcggcaaca aaggtccata
     2041 aaagccaacg cattcgatac aaacgccctc cccgcagcaa catgagcccc cccgccgcaa
     2101 gaccgatgag gatcatgccg atacccacca tcgcgtgata ggactgaaac acaatctgca
     2161 ccggtggccg atcctcaggc gcaaacgcac ggagcccggt cacgggagct ttgaagtccc
     2221 gatgcaacat catgctgagc atccccggaa ttcctatgcc gatgaccttc tcgttttttt
     2281 cgtcgaccca accgaacaaa tacaaatccg cgggcgccga agcctcgtag tgtccctcga
     2341 acgccgcaag cttggccggt tgattggccc ctaccccctc cgcgctcgta tgcccggtcg
     2401 ccaactgccc aagcgaagaa accaacgcca acacaagggc aagctgcatc gactttttgg
     2461 caatcttttc atggcgcccc cgcagtaaat aataggcgct caccgagagc accaaccatg
     2521 cccccgcttg ccaacacccc atgatcgtat gggaaatccg atccaccgag gaagggttga
     2581 ataccacggc ccaaaagtcg acgatttcgg ctcgcgcctg acttccttcg ccaacgatat
     2641 gatgccccgc aggcgtctgt tgccaagaat tggcgatcac gatccacacc gcgctcaagt
     2701 gactccccaa cgccaccatc gccgtcgcaa aaagatgcac gcccggcttc acccgattcc
     2761 aaccaaagag cagcaacgca aggaatcccg actccaaaaa gaacgcaaat atcccttccg
     2821 ccgccaaggg actcccaaag acatccccca cgaatctcga atacgtcgac cagttcgtcc
     2881 caaactgaaa ctccatcaca atgcccgtcg ccacgccgac agcaaagatc aacccgaaca
     2941 cccgcaccca gaacttcgtc atctgctcgt agtccgggtc ccgggtcttc acgtacgtcg
     3001 cctccatggc caccaacagc accccaagcc cgatgctcaa cgggggaaat acgtaatgca
     3061 ccatgagggt gaaagcgaat tgcagtcgtg caagcagatc cacgtccatg gggactcctc
     3121 ggaaaggcaa gacgccgggt ctagtcgcaa accgtcctag agcttggccg cttggcggtc
     3181 aaccgatccg ctacaaccca ggaaatcaca gccattcgag aatagcccat cgcggaaaag
     3241 agccgatctt ttgcactcgc cacaccacct ttctcgaatc atagtctatg gattcaggct
     3301 caagccttct tgctctacgc tacgctcagc gtgagttcat cgatgctcaa accggaacag
     3361 ctcgccctgc ccttcccaga gatccagacc gccccgcgca cgatcatcgc gcgggttcat
     3421 cgaatcttcg ccaatcaaag catgaaaatg ggggatgtcg agtggatcgg cttcgacatg
     3481 gatcatacga tcgccatgta cgacccatca gccttggatt cgctttgtat ccattcgatg
     3541 atcgataggc ttcgccaaca aggtttccct cacgccccca ccaacgtcga cccctccttt
     3601 tgcatccgag gcttggtcat cgacaaacga ctcggccata tcctcaaact ggaccgatat
     3661 cgagtcgttc gaaaaggatt ccacggtttg cgggagcttt cgcccgacca gcttgccaaa
     3721 acctatgaag cccgacgaat ccgcgtgggg acaccacaat tttttggcat cgatacgctt
     3781 tacgcacgaa gcgaggcttc tttattcgcc gcatggatcc aatcgctcga gcagcaacgc
     3841 cacagcatcg acccttccca tctgttcgac cagatcagaa cctgcgccga cggtcttttt
     3901 cgtgatggca ccatccctcg gctcattacg gatgacctca ggcgcttcat ccgaaaagac
     3961 ccacaactcg cctccacctt tcacaaacta cgttccggcg ggaaaaagct ctttcttctc
     4021 accaactcgc cgtggccttt cacccatgcc gtcatggatt acctgctcgg cagcgaaatg
     4081 cccgaatatc ccacctggag acactacttc gacatcgtca tcacctcggc caacaaacca
     4141 caattcttcc tcgaacgtca gcccctcatc gaacaagatg gagactcgaa acgccccgtt
     4201 gtcctgcgcg cttcgacctc gttcgaacga ggaaaatcct atgaaggcgg caatctgtcg
     4261 gacttcacac gattgctcgg cgttcccggt gaccggatct tatatgtggg cgaccatatc
     4321 tacggcgata tcttgcgttc gaaaaaagaa gcctcctggc gcacgggcct cgtgctgcac
     4381 gaactcgatg atgaacttcg agcccatgaa gcctcccttc ccgatatcca acgcttgcaa
     4441 gacttgacct cccaacgaga agagttggaa gacgaacttc gtgtccacca aacgcttttc
     4501 aaagatcagt cacgacaact cgagtcggtt cgcgccacac gggacatcgc catgacttcc
     4561 gctgtcgaag tgcaacgcca actcgagaaa cgtaccgtcg aacgcgttcg cggtttgctc
     4621 caaagcaacg aaagcgaaac ccgcgatatc gaggcgcgga tcgaacaacg cttccaccca
     4681 acctggggcc ccctgctcaa agaaggcacg gaactgtcca gcttcggaca acaggtagaa
     4741 gcctacgctt gtgtctatac gtcccgcgtt tccaatttgc ttttttattc tcccatccag
     4801 cacttccgaa gcccgagaga tctcatgcct cacgagtttt gatcgtcgcc tccgaatgaa
     4861 aataccaatc ccctttccac ggccccaaca tcgatcaatc gtccaatgtc ttgcccgtga
     4921 aatacgtcct gtcaaagacg gtcggcaagg cgtgaagcgg cacgttaacc ttcgtcttcg
     4981 tccagcgcgt gggagcatac atctccccgg tgttgaggta tttttggttt ttgctgttga
     5041 tgacgcctga atctcgcttg tagggcatgg taggctggcc aaattcatcg aataccgcgt
     5101 atcgataatc caaaccaccc gccccttccg ctaatccccc ctgcgatgaa aagtacttga
     5161 atcccaccac ccgttcattc caaaccgaat actccgtgcc ttggatatcg atgaacgata
     5221 ggaaaatcac cgcgtgaccc gttccattgg tgcgattcag attgatgaat gatcccggcc
     5281 ggagctgctc gaaagatacc gtctcaccca tcccaaaatg cacgagcgca tccgcggtcc
     5341 catacgagtc cagcgaatga ttgacccaaa tatgtgcctt gatgtgatcc gaatgcagcg
     5401 tctgccaact ctcgatagga agaaaatcaa aaaccgtcgt gtctcccgta tcctccgccc
     5461 atatcgtcat cgccgtgaga atcacttcca tcgccgccgc cacacacatc gtcttcgctc
     5521 cccccgttcg gtagatcgtt ccgtacgttc catacggtat gtcatgcgtc aacaccgccg
     5581 agtcgtatcc gagcaaccga tactgcgcat cgaggtaatc gaccgctgaa agcacatagt
     5641 catgaaagta atcccctggc ggcaccttgc gcggacgaag actcaatcgc aaagaatacg
     5701 ggcctgccag ttcttggccc gacgatacga atgtgtccgc cgtcacgtag tacgttcccg
     5761 gctccaacac cgcgtataac gccgtattcc ctcgtttttc caacgtgaga ggctcgatgg
     5821 acgacagcag atgcaagtcg acatccgttc cttgcggctc ggtaaaagca atcgtagcgg
     5881 caagccgcgc tggctcgtcg atgcggaatc gatacacaat ctcacgaccg gattcatcga
     5941 ggtgttcgaa cccaggataa gcgtcaatcg catcggatat cgcctcccgt gtgtcccgat
     6001 tatcatgaaa aacgaaaggc aaaggcagcg cggcgtccgg atctttgcca atgacgatcg
     6061 gatcatccaa cgtccccgca tgccatgccg taaaccccac cgtcaaatca tagggccccg
     6121 ccatcgcgcc actgctgctc acgtacgtgt ccaacacaag gtagtaccga ccaggttcga
     6181 gtagttcatc gacacttcga tcatccctcg ccacaagcgc gatcggatcc acggaagaca
     6241 acaaatgtac gtcaacgtcc gtccccgacg gctccaacgc taaagatgcc ttgacgcgtg
     6301 ccttgtcttg tagagcaaac atatacacga attctggacc tgattcgttc gtcttttcga
     6361 accccggata gctgtcgacc accgatgagg tcgcgtcttt cgtgttgcgc gcatcatgaa
     6421 aatccggcag tcgaggatcc gcgggaatga aaaacggatg ctcgaaggtt ccgtcttgat
     6481 actgacaagc atccccttga ccatccccat ccgtatccat ttgatctgga tttggcatat
     6541 ccgggcagtt gtcgacgtca tccggaaccg agtccccatc catatccaca atcgggacac
     6601 tgccctcggt ttgcgccgcc tccgccgccc cttcacgggc actatcctcg ggcacccccg
     6661 acgcatcggc tgccgaattg ccggaatctc ccccccaaga ccatgaagaa gcgtcatcac
     6721 ccgaactaca agcggataat gacaacagca aaccaattcc ccatatcaat cgatatccca
     6781 tggacatggc agatctcgct tgtgaaagga tcgcttctag ctaggttgac acatcggcac
     6841 gccaatgcat ggtacaacca ttccattgcc catttcccat cacggactca tcgggttact
     6901 ctttcccaaa aatgacgcgg tccaatcgac tccctccggt tcttcctccg cccaccaaga
     6961 cggtgggaga cgatgagtcg acattgattg cgcctccagc acaactgcta gagcgagcga
     7021 gcagaccgcc tcccgcacgc acaacggtcc cccctccccc cggctccggg gtcccaccac
     7081 ccgtacgcac atcaccccct ccaccggttc gcccctcttt ctctcgtgga tcaccgccgc
     7141 caccgagcgc tgcgaaaccg ccacatatcg aaaaattggg acgtcccaca ccggaatcgt
     7201 gggaaatgtc gcaactccca ggcattgcct ccaccaagaa cataaccgtt cgccccaccg
     7261 ggcaaccacc tcttgcccct acctccgacc aacccctgcc caaatccgtc ggcctgaaag
     7321 tattgggaat gtggatcggt ggcgcggccg tcatcggcct tttggtcagc gccatcggag
     7381 catggtggtt ccttcccaaa ccccaaactc atcaccaccc catcgcctcc gccaccgtcg
     7441 cctccccgac acgtacacgc tcgactccca gcggccccgc ctcgacagaa tcggaaacca
     7501 ctccttccgc actatcattg ccctctcttg ccgaacgggc agcacaagga gaccctgatg
     7561 ccatcgacca gatcgaacgg cgacccgcgc ccaaccgcac tttcaatgaa tcgttagcct
     7621 gggcgcgagg caagtccgtc ttcaaacacc gtcagttgga gttgattgcc aaagaattac
     7681 gcgccgatct tcctctgtcg gcggaccgac gtaccttggc aaaactcaaa ccattcaccg
     7741 aagaccctga aaccgccacc gaagcccttg ccatcctcgc ttctttacga acacctctgg
     7801 ccgtcgacat gctttacgac gtgtgggttc gacgacgaga tgggacgatt gccttcgagc
     7861 ttgcgaaaag cttgctgtat acaccggaga ttcgcgaggc cgcctcggat gccctcgctg
     7921 tcgttctcga tctacgcgct gcctccacat gcgaacaagt cgcggaattg cttccacgag
     7981 tacaacaacg agccgatcga cgcgcgttgc ccatgcttgg caaactcact tcgcgatacg
     8041 gctgcggccg gctgggaggt cacgattgtt acccttgctt gcgaggctcc aacgcggtga
     8101 acgacgccat cgcggcggta ggaaaccgtc cggccccaga tatttgaatc aggataacgg
     8161 aaccgtccgc gccacgaccg ctcacgacaa atcacggttc tctaaaaaac cgtggaactc
     8221 gacgagatat tcccgtgaga atctcccatg agatggtatc cgcatggttg gcaatctcat
     8281 cggcggtgat gcaatcagaa ccgagcggcc cttgttgagg accaagcacc gcaacctcat
     8341 cgcgcaacga agccccaggc acatcggtca tatcgatcat cgtcatgtcc atcgagacat
     8401 ttcccacgat cggagctgtc gttccgcgca cgagcatccg tcccttgttg gacaagctcc
     8461 gcggcaaacc at
//
LOCUS       DAOSOR010000207         8446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8349_length_8446_cov_5.133953, whole genome shotgun sequence.
ACCESSION   DAOSOR010000207 DAOSOR010000000
VERSION     DAOSOR010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8446
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8349_length_8446_cov_5.133953"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PK710_12330"
     CDS             complement(<1..314)
                     /locus_tag="PK710_12330"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT10554.1"
                     /translation="MKRGDIVAGKYRLKRVLGKGAQGHVWEGVHEQMGTPLAIKMLDP
                     NLAGRADYVSRFKREARAAAALRSPHVVQIFDFGIWQEVPYIAMELLDGEDLGERLDR
                     VRR"
     gene            482..>2144
                     /locus_tag="PK710_12335"
     CDS             482..>2144
                     /locus_tag="PK710_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10555.1"
                     /translation="MLPLSSLLLQRGVATLAEVETALARQSVQGGDLVTNLLEVARVD
                     EAKLIETIADFEGLPAAPTGPLPRPEPDALSVVPITVASKIPMLPQRLHGDCLSVVVA
                     EPIPRSMEQELSFSLGIQFEQSLSCLSRVLQGLAAAYRVPLHRRHARVVARLDGTDDP
                     YPSVAPPSPVLSSLVPSRLSHGPAASSEEPSHEPWVRPPVTVQSSAALSRALAANAST
                     KARRGPFELIAAREALESAGTNTEIVAIFFDFAKQFFEYVALFMVRDDLAEGLAAAGP
                     GVSRDRIRSIGVPLELPGILSRARDEKAHVLDIPANDGIDAVFRQDLQRPMRARVLVA
                     PLAVRSRVIALLYGDDGRSDVDLNLVGDVLALVPLVSSALETLILQKKLQQRSKPSQG
                     AKTPEKPVEKRKRTTLRGLPVQDTRPQTIPPVVVPSASPGPKSSTTSPFVPVVEPPKA
                     PPVPNIEDVLPPDPMADHPAVAEPEVGLRSPSTLLGLGGDSSDAVVRELSDLESEPSP
                     AAVQEPEKALEADTLEQEGAAPDEDETGAASEPSPAAVQEPEKALE"
     assembly_gap    2145..2244
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2245..3690
                     /locus_tag="PK710_12340"
     CDS             <2245..3690
                     /locus_tag="PK710_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10556.1"
                     /translation="VQEPEKALEVDTLEQEGAAPDEDEPVAASEPSPAVVQEPEKALE
                     ADTLEQEGAAPDEDEPVAASEPSPAAVQEPEKALEVDTLEQEGAEPDEPLAEPPTVEE
                     GIREDAAAEKTVNEDDAPSISYSEMDEYDGGDLMNVVLAELQRSPTPEAVEESTQAVV
                     RGPLEVPKSQSPADLGLPQVILNLEDEVEELVDKLESTDIPESQAESIRAELRSYGQG
                     WIEPVLSRFPGRAVKIPELFTDESLMVSERGGLLRFVVEQGSMVAQRLLDRMAESGPE
                     TRVWLVLTLAEIGGKEAREAMNLALFDSHERVRSAARRVLRSVVGLRAWANGVRSRVR
                     KIATEDTASDRRLLAIQALGEIREGSSVPILLGALDSDVAAIREAAVDGLRSITRQSF
                     DDHPEAWKTWWKDNSQRARYDWLVDALCASDERQREEANLELIDIVGENLGFDPMASE
                     SAQERAIQAYRTWWDDEARRSSKSKRKPNAS"
     gene            complement(3816..5234)
                     /locus_tag="PK710_12345"
     CDS             complement(3816..5234)
                     /locus_tag="PK710_12345"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT10557.1"
                     /translation="MMAKDDRFRITFLGATRTVTGSMHRLDVRQHGFLIDCGMFQGRR
                     HESMHRNRNLPLKAVQADALLLTHAHIDHCGNIPTLVKRGFVGNVHCTSATADLCGAM
                     LKDSARLQLADARWLNEKYADDPNWEPIEPLYDEDDVKRAMERFIPHDYEEVFELSPV
                     AHAHFIDAGHVLGSASAIIDVKLNGHRRRVAFSGDIGRRNMPILRDPIPPQNPDYVVM
                     ETTYGNRMHAPSELMQDQLYDVIKTALSRGGKIIIPSFALERTQEIVFALNQLRKAKR
                     LAAIPVYVDSPLAHNLTQVFRRHGECFDSETIAFDDEHGDPFGFDMLTFVNSVDESKQ
                     LNEKPGPMIIISASGMCEGGRIVHHLRNSIEDDKNTIVIVGYQAQHTLGRRIVERHPR
                     VRIFGVDRDLFAQVRVLNAFSAHADRDELLWWTDACGSQVRQHFLVHGDEDQSQAFQG
                     HLEQRHKRAVVPRENEVIDLVY"
     gene            5440..5922
                     /locus_tag="PK710_12350"
     CDS             5440..5922
                     /locus_tag="PK710_12350"
                     /inference="COORDINATES: protein motif:HMM:NF015941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HOT10558.1"
                     /translation="MELHVVKLDKPEEVNLVFGMSHFIKTVEDLHEALATAAPGIKFG
                     IAFCEASGPRLLRHSGNDDAMRKLAIDNLEKVNAGHSFLIMLSGVFPIHVMRAIRSVP
                     EICRIYCATANPVEVIVAQTEQGRGVMGVIDGSPTVGVEDDEQIAKRKGFLRTIGYKL
                     "
     gene            5927..6802
                     /locus_tag="PK710_12355"
     CDS             5927..6802
                     /locus_tag="PK710_12355"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT10559.1"
                     /translation="MDYVGAIFRPPSEADSMLLQVTVGCSHNKCSYCAMYREKRFRPK
                     PWNVIEKDILEAASIGPRFRKVFLCDGDALILPMGRLVEILTALRQHLPWVERISLYG
                     DTRSVLRKSVSELSQLRQLGLTMVYHGVESGDDEVLRAVEKGGTRSEVVQTAKCLREA
                     AMAHSVIIMLGLGGVQRSEQHIKATASLLSEIDPPFVGVLTTTVVPGTPLAQAEHAGS
                     FVLPSKFDMLRELLYLIENARFSKCRFSSNHASNYLPVRCMLPRDRQSVLSALRAVID
                     KGDDSVLRPEWSRGL"
     gene            complement(6837..7217)
                     /locus_tag="PK710_12360"
     CDS             complement(6837..7217)
                     /locus_tag="PK710_12360"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOT10560.1"
                     /translation="MSHDSVPPPVDRRTSIRQAANTPVALLCESWAMLALIEDVSESG
                     LLLLTQEPIEVGTTVQVHLLLGSHVHPPLKATASIVRSERREISTSYWTHQVALQTDH
                     REGPWKQALDEIVQRQASLRNTDI"
     gene            complement(7273..8232)
                     /locus_tag="PK710_12365"
     CDS             complement(7273..8232)
                     /locus_tag="PK710_12365"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT10561.1"
                     /translation="MPKHPCLSSFITLTAVTMAIAACTEPSKTLPEAAMSDTAPSASS
                     APSPPTAQPVPPTSSAAAEPSTPCPKDMVLVPAQKPYCIDRWEAVVVDKKSGRRFSPY
                     YSPLPKSARSIRDIWQKKRLEVGDATARAMPLPPLPEWQLSDDTIPMAVSLPGQTPQG
                     YLSGKVARMVCENAGKRLCSPSEWVHACRGVANRPFPYGDTYQQGACNIFRFTHPAGA
                     LHDDVTTGHLDPRLNQVKDKQGLLLRPTGETKTCASQWGNDAVYDMVGNLDEWVEDEK
                     GMFLGGFFSRSKKDGCASVVKNHPNEYWDYSLGVRCCRAMQEQ"
BASE COUNT         1756 a         2238 c         2480 g         1872 t
ORIGIN      
        1 cggcgtactc gatccaggcg ttccccgaga tcttcgccgt cgagaagctc catggcgata
       61 taaggaacct cctgccagat accgaaatcg aagatttgta ccacgtgcgg agatcgtagg
      121 gccgctgccg cccgcgcttc ccttttgaaa cggctcacat aatccgctcg gcccgccaag
      181 ttcgggtcga gcattttgat ggcaaggggc gtgcccatct gctcgtggac cccttcccac
      241 acgtgtccct gggcgccttt gcccaagacc cgtttgagac ggtacttgcc tgctacgatg
      301 tcaccgcgtt tcacggcatt ccttgcgaga tagggtagac gcaattgaat gaatctgccc
      361 agagaacgga cgaaatatac tccaaaaccc aaactatcgc gccgcttcac ccagaatttg
      421 acaatgtatg tgaatacgcg acaagctgat aagtcacgaa gcgagagaag gtcgttcgtc
      481 gatgttgcct ttgagttccc tgctcctgca gcgaggtgtt gccaccctcg ccgaggtgga
      541 gactgccctc gctagacagt cggtacaggg tggtgatctc gtcaccaacc tgttggaagt
      601 ggctcgggtc gacgaagcca aacttattga aaccattgct gattttgaag gattgcccgc
      661 ggcacccaca ggcccgctcc cacgaccgga acccgacgcc ttatccgtcg taccgatcac
      721 cgtcgcctcg aaaattccca tgctccctca gcggttgcac ggtgattgtc tgtccgtggt
      781 cgtcgctgaa ccgatcccgc ggagcatgga gcaagaactc tctttttctc ttggaatcca
      841 gttcgagcaa tccctctctt gtctctctcg cgttcttcaa ggcctggccg ctgcctatcg
      901 cgtcccgctg caccgacgac atgcccgtgt ggtggctcgt ctcgatggca ccgatgatcc
      961 ctatccaagc gtggctcccc cgtcgcccgt gctaagctcc ctcgttccgt ctcggctttc
     1021 ccacggacct gccgccagtt ccgaagagcc atcgcatgag ccgtgggttc gacctcctgt
     1081 cacggttcaa tcatcggccg ccttgtcccg tgcgcttgcg gcaaatgcgt ctaccaaagc
     1141 acggcgtggc ccctttgaac tgattgccgc ccgggaagcc cttgaatccg ccggcaccaa
     1201 caccgagatc gtcgccatct tcttcgactt tgcgaaacaa ttcttcgaat atgtcgcatt
     1261 gttcatggtg cgcgacgacc tggcggaagg attggccgcc gcgggaccag gcgtgagccg
     1321 agaccgcatt cggagcatcg gcgttcccct cgaactgcca ggtatccttt cccgtgctcg
     1381 cgatgaaaaa gcccatgtgc tcgatatccc cgcgaacgat gggatcgatg ccgtttttcg
     1441 acaagaccta caacgcccga tgcgcgctcg cgtgctcgtt gcccctctgg ccgttcgctc
     1501 ccgtgtcatc gccttgttgt atggcgatga tggtcgttcg gatgtcgatt tgaatcttgt
     1561 cggcgatgtg ctcgctttgg tgcctctggt aagttcggcc ctcgaaacac tgatcctcca
     1621 aaagaaactt cagcagcggt ccaagccatc ccaaggagca aagactccag aaaaaccggt
     1681 tgaaaaacgt aaacgcacga ccttgagggg actgcctgtt caagataccc gcccccagac
     1741 cattcccccg gtcgtcgttc cttccgcttc tccagggccc aaaagctcta cgacatcgcc
     1801 ttttgttccg gtggtcgagc ctccaaaggc ccccccggta cccaatatcg aagatgtttt
     1861 gcctccggac cccatggctg accaccccgc tgtcgcggag ccagaggtag gcttgcgcag
     1921 tccaagtacg ctgctcggcc tgggagggga ttcgtcggat gccgtcgttc gagaactgtc
     1981 ggacttagaa agcgaaccct cccccgccgc agtccaagaa cccgaaaaag cgttagaagc
     2041 cgatacgttg gagcaagaag gggcggcgcc ggacgaagat gaaaccggtg ccgcgtcgga
     2101 gccctccccc gccgcagtcc aagaacccga aaaagcgtta gaagnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnngtccaa gaacccgaaa aagcgttaga agtcgatacg
     2281 ttggagcaag aaggggcggc gccggacgaa gatgaacccg tcgccgcgtc ggagccctcc
     2341 cccgccgtgg tccaagaacc cgaaaaagcg ttagaagccg atacgttgga gcaagaaggg
     2401 gcggcgccgg acgaagatga acccgtcgcc gcgtcggagc cctcccctgc cgcagtccaa
     2461 gaacccgaaa aagcgttaga agtcgatacg ttggagcaag aaggggcgga gccggacgag
     2521 ccgcttgcag aaccccccac ggtggaagaa gggattcgag aggatgctgc cgcggaaaaa
     2581 acggtaaacg aggacgatgc cccaagcata tcgtattccg aaatggatga gtatgacggg
     2641 ggcgacttga tgaatgtcgt tcttgcggag cttcagcgtt caccgacccc agaggcggtg
     2701 gaggaatcca cccaagcggt ggttcggggg cctttggaag ttccgaaaag ccagtctccc
     2761 gccgatttag ggctaccgca ggtcattttg aatttggaag acgaggttga agaattagta
     2821 gacaagttag aatctacgga tattcctgaa tcgcaagccg aatctatccg ggcggagctg
     2881 cggtcctatg ggcaagggtg gattgagccg gtgttgagtc gatttcccgg gcgtgccgtc
     2941 aaaattcccg agttgttcac cgatgaatcg ttgatggttt cggagcgagg gggcttgctg
     3001 cgtttcgtgg tggaacaagg cagcatggtg gcgcaacggc tattggatcg catggctgaa
     3061 tcaggtccgg agactcgtgt atggttggtt ttgacgcttg cggagatcgg agggaaagaa
     3121 gcacgggaag cgatgaattt agcccttttc gattctcacg aacgcgtgcg gagtgcggct
     3181 cggcgagtgc tgcggtccgt ggtcggtttg cgtgcgtggg ccaacggtgt tcgctccagg
     3241 gtgcgaaaaa tcgcgacgga agacaccgct tccgaccgtc ggctgctcgc cattcaagcg
     3301 ctcggcgaga ttcgagaagg ctcatcggta ccgatcctat tgggagcctt ggactctgac
     3361 gttgccgcga tccgagaagc tgcggtcgat gggttgcgct ccatcacccg gcaatcgttc
     3421 gatgaccacc cagaagcttg gaagacgtgg tggaaagaca atagtcagcg agcccgatac
     3481 gattggctcg tcgatgccct ttgcgcgagc gacgagcgtc aacgggagga agctaatctg
     3541 gaactcattg atattgttgg tgaaaattta ggattcgatc cgatggcctc ggagtccgcg
     3601 caagagcgtg ccattcaagc gtatcgaacc tggtgggatg acgaggcgcg tcgttcgtcg
     3661 aaaagcaagc gcaagcccaa tgcttcttga gcgggggacg gaaacgctga agtcgagctt
     3721 ggggtttggg gcgaatggga gttggccgta ggcaagtcga tggggattcc gatggtcgtg
     3781 gaggacgaag cggttgggta gccgcgtttg ggttgtcaat acaccaggtc gatgacttcg
     3841 ttttcccgag gaacgacggc tcttttatgg cgctgttcga gatgaccttg gaaggcttgg
     3901 ctctgatcct catccccatg caccaagaag tgctgccgaa cttggcttcc acaagcatct
     3961 gtccaccata ggagttcgtc gcggtccgcg tgagccgaga aagcattgag tacgcgcacc
     4021 tgcgcgaaaa ggtcgcggtc gactccaaag attctcacgc gcggatggcg ttccacgatg
     4081 cgtcgcccca acgtatgctg tgcctggtag cctacgatga cgatcgtatt tttatcatct
     4141 tcgatgctat tgcgaagatg gtggacgatt cgaccgcctt cgcacatccc ggaggcgctg
     4201 atgatgatca tggggccggg tttttcgttg agttgtttgg attcatcgac cgaattcacg
     4261 aacgtgagca tgtcgaaacc aaaagggtct ccgtgttcgt cgtcgaaggc gatggtttca
     4321 gaatcgaaac actcgccatg ccttcgaaaa acttgggtga ggttatgggc gagaggtgag
     4381 tcgacgtaga ccggtatggc ggccaaacgt ttggcttttc gcaattggtt gagagcaaag
     4441 acgatctctt gggttcgttc gagggcgaag ctcggaatga tgatcttgcc tcctcgtgac
     4501 aaggccgttt tgataacgtc atacaactgg tcttgcatga gttcggatgg ggcatgcatc
     4561 cgattgccgt aggttgtttc catgacgacg tagtccggat tctgcggcgg aatcggatcg
     4621 cgtaaaatcg gcatatttct tcgaccaata tcgccggaga acgcaacgcg acgtcgatgg
     4681 ccgttgagtt tgacatcaat gatggcggat gcagatccga gtacatggcc cgcatcgatg
     4741 aagtgggcgt gggcgacggg cgatagttcg aagacctctt cataatcatg agggatgaag
     4801 cgttccatgg cccgcttcac gtcgtcttcg tcgtacaggg gctcgatggg ttcccagttt
     4861 ggatcgtcgg cgtatttctc gttgagccaa cgcgcatcgg ctagttgcaa tcgcgccgag
     4921 tctttgagca tggcgccgca caggtccgcg gtagcgcttg tgcaatggac attgccgacg
     4981 aatcctcgtt tgacgagggt aggaatgttg ccgcagtggt cgatatgagc gtgcgtgagc
     5041 aaaagcgcat cggcttgcac ggctttgagc ggaagattcc gattgcgatg catggactca
     5101 tgccgacgtc cttggaacat accgcaatcg atgagaaaac cgtgctgtcg gacatcgagc
     5161 ctgtgcatgg atccggtgac ggttcgtgtg gcgcccagga acgtgattcg aaaccgatcg
     5221 tcctttgcca tcattgagaa tgacctccac gtggtatgcc gaagaccttg ttgtaactcg
     5281 aattgccggt ccagttcgag gtccgttatg gtggactcgt aaaaagcgcc gtatcgatga
     5341 gaaaatagtc attgattacc gggcaaactg gttgtctggt ctccaaggct tgaaccccgc
     5401 gtctaccctc gtacgatcgc aatatttcag gaggattcca tggaacttca tgtcgtcaag
     5461 ctcgacaaac cggaagaagt caatctcgtc ttcggcatgt ctcacttcat aaaaaccgtg
     5521 gaagacttgc atgaggcttt ggcgaccgcc gcgcctggaa tcaagttcgg catcgctttc
     5581 tgtgaagctt ccggaccgcg gctgctccgc cacagtggaa acgatgacgc catgagaaag
     5641 ctcgccatcg acaacctcga aaaagtcaac gcgggtcact catttctcat catgctttct
     5701 ggcgttttcc ctatccatgt gatgcgtgcc attcgcagtg tccccgaaat ctgcagaatc
     5761 tattgcgcca ccgcgaatcc cgtcgaagtc atcgttgccc aaacggaaca agggcgtggt
     5821 gtgatgggag tcatcgatgg ttcgccgacg gttggcgtgg aagacgacga gcagatcgcc
     5881 aagcggaaag gttttttgcg gactatcggg tataagcttt aggcgcatgg actacgtggg
     5941 cgccattttt cgtcctccca gtgaggccga cagtatgctt ttgcaagtga ctgtcggttg
     6001 cagtcacaac aagtgcagct attgcgcgat gtaccgtgaa aaacggtttc gccccaagcc
     6061 atggaacgtt atcgaaaagg acattctcga ggctgcgtcc atcggaccgc gctttcggaa
     6121 agtgtttctt tgcgacggcg atgcgctcat tcttcccatg ggccgattgg tcgagatctt
     6181 gacggccctt cgtcaacatc ttccctgggt tgagcgaatc agcttgtatg gggacactcg
     6241 gagcgtgctt cgaaagtccg tttccgaact gagccaatta cgtcaactag gtctgacgat
     6301 ggtgtatcac ggtgtggagt ccggtgacga tgaagtcctg cgcgcggtgg aaaaaggcgg
     6361 cacgagaagc gaggtcgtgc agacggcgaa gtgtttgcga gaagcggcaa tggcacattc
     6421 tgtcatcatc atgctcggtc ttggaggtgt gcaacgatcc gagcagcaca tcaaagccac
     6481 cgcttcgttg ttgtctgaaa tcgatccacc tttcgtgggg gtgttgacca cgaccgtggt
     6541 gccgggcaca ccccttgccc aagccgagca cgcaggctct ttcgtgttgc cgagcaaatt
     6601 cgacatgctt cgagaattgc tctatcttat tgaaaacgca cggttttcga agtgccggtt
     6661 ttcgtccaat catgcatcca actatcttcc cgttcgatgc atgcttccga gggatcggca
     6721 gtccgtgttg tccgcgctgc gtgcggtcat cgacaaagga gacgatagtg tgctcaggcc
     6781 ggaatggtca cgagggctgt agatcctggt gatggatcga ccttgtggac acaccatcat
     6841 atatcggtat ttcgcaaact tgcctgacgt tgcacgatct catctagcgc ttgtttccac
     6901 ggaccttcgc gatggtccgt ctgcagcgcg acttggtggg tccaatacga tgtgctgatt
     6961 tcccggcgct ccgatcggac gatcgaagct gttgctttca acgggggatg cacatgagaa
     7021 ccgagtagca aatgaacctg aaccgtcgta ccgacttcga tgggttcttg ggtaagcagc
     7081 aacaggccag actcactgac gtcttcgatc agcgccagca tggcccacga ttcacatagc
     7141 aaggcgaccg gagtattcgc ggcttgtcga atggacgttc gtcgatcgac gggaggagga
     7201 acggagtcgt gtgacatggg tcagctcttt ggaaaggtgc agcattgtat catgggccat
     7261 cgtcatggct gtctattgtt cttgcattgc gcggcagcat cgcactccca aggagtaatc
     7321 ccaatactca ttggggtggt ttttgacgac ggacgcgcag ccgtcttttt ttgatcttga
     7381 gaaaaatccg cctaaaaaca tgcctttttc atcttcgacc cattcatcga gattgccgac
     7441 catgtcgtag acggcgtcat ttccccactg gcttgcgcaa gttttcgttt ctccggtggg
     7501 gcgtagcaac aggccctgtt tgtctttgac ttggttgagt cgggggtcga gatggcctgt
     7561 ggtgacatca tcgtgcagcg cgccggcggg gtgagtgaaa cgaaaaatat tgcatgcgcc
     7621 ttgttgatag gtgtcgccgt aggggaaggg tcggttggca actccgcggc aggcatgcac
     7681 ccattccgaa ggggagcaaa ggcgcttgcc ggcgttttcg cagaccatgc gcgcgacttt
     7741 tccggaaagg taaccctgcg gagtttggcc ggggagcgac acggccatcg gaatggtatc
     7801 gtcgctgagt tgccattctg gcaaaggtgg taacggcatg gcccgggccg tcgcatcccc
     7861 gacttcaagg cgtttcttct gccagatatc gcgtatcgaa cgagcgcttt tgggtaacgg
     7921 agagtaatac ggcgaaaaac gccgtcccga ttttttgtcc acgaccacgg cttcccatcg
     7981 atcgatgcaa tagggctttt gggcggggac aaggaccata tcctttgggc atggcgtgga
     8041 aggttccgca gcagcgctcg acgtcggtgg aacgggttgg gcggttgggg gcgaaggtgc
     8101 cgaagaagcg ctgggggcgg tgtctgacat cgcggcttct ggcagggttt tggatggttc
     8161 cgtgcaagct gcgatagcca tggttacggc ggtgagtgtg atgaatgagg ataggcaggg
     8221 gtgtttcggc atgagaacca accatcgttg cgtgctgcgg cgtgcttttt tggggggatg
     8281 accctacact tgcggcgagg agcccatgtc cctgagaatc gaccaggacc actcgcgttt
     8341 tcgtgccatc gtacgtggca aaattcgcga aaatctcaag aaatacgtgt ctcacggcga
     8401 gatgattggc cgcaagggca aagacctggt gaccattccc atcccc
//
LOCUS       DAOSOR010000208         8440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8358_length_8440_cov_4.033989, whole genome shotgun sequence.
ACCESSION   DAOSOR010000208 DAOSOR010000000
VERSION     DAOSOR010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8440
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8358_length_8440_cov_4.033989"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PK710_12370"
     CDS             complement(<1..559)
                     /locus_tag="PK710_12370"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HOT10562.1"
                     /translation="MSSDRLVPRGRLSRLAHLAAAGTRLGTRKLRGSNESLSAADTAR
                     VLGDLRGLAAKFGQMAGYVDGVLPAAQRSTYGEALASLFQHAAPSPREHIESLVRSEL
                     GAYAHELFASFGEQPVASASIGQVHRARLHDGTDVAVKVQHPGIRNALQSDLANLGWL
                     ESLFGSYLGAGFNAHGVVEEFRERFL"
     gene            complement(643..1251)
                     /locus_tag="PK710_12375"
     CDS             complement(643..1251)
                     /locus_tag="PK710_12375"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT10563.1"
                     /translation="MSLKQLRLSARERSQVQTHQRLLDQGLRLFATQGISRTKAIDIA
                     RAASVAVGTLYLHFQDKDGLLHEILITGLGKLRAPLRALAEERHPSYSHSLRRHVEVM
                     VDFVNNRPDLSKVMFHPEVLHTPVGKDVVDFMTLTMENLLREGAACGNVRKDIDPVVA
                     AQALAGMLLHVLWWWAQDPSHAPQHHVVDTLAAYHLATLLNN"
     gene            1748..2671
                     /locus_tag="PK710_12380"
     CDS             1748..2671
                     /locus_tag="PK710_12380"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOT10564.1"
                     /translation="MTANQDAGESVLVAKEFLSSKVTKYVVRARDIARRRRPGQFVIV
                     RSFAESERIPLTIADGDEQEGTITLIVQEVGKSTAEMAHMMRVGDRFADVLGPLGMPT
                     HIEKVGRVIAVGGGIGIAPLHPIVQGFRKAENEVIGIIGARSRDLLIMEEEMKRACHR
                     LRIVTDDGSYGDKGLVTDALRAEVEASGKPALCVAIGPLVMMQAVSKVTKELGIKTLV
                     SLNPVMVDGTGMCGGCRVTVHNETKFVCVDGPEFDAHGVDFDELRRRQAFYAPQERGA
                     YDLFLKKHPFGAEHCKVHRLVDEAQRGKEAK"
     gene            2668..4158
                     /gene="gltA"
                     /locus_tag="PK710_12385"
     CDS             2668..4158
                     /gene="gltA"
                     /locus_tag="PK710_12385"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOT10565.1"
                     /translation="MSTAHDKAVVTPKIDLPAGAEPKLSNKERMAIPRQVMPEQSAEE
                     RIYNFLEVPLGFEENVARIEALRCIKCKKPTCIDGCPVGINIPGFLEKIAEGDFEGGA
                     RVLKQDNILPAICGRVCPQEEQCEKQCIVGKKNAPVAIGRLERFLADWEREHNKVQPL
                     VPGEPTGKKVAIVGAGPAGLTAAADLVRFGHKVVLYEALHKPGGVLVYGIPEFRLPKK
                     IVEFEVQQLEKAGVELHNSVVIGQTITMTELQETYDAIFVATGAGLPYFMNLPGENLN
                     GIYSANEYLTRVNLMKAYDPDSDTPVAKSKNVAVVGGGNVAMDGARTAKRLGADNVYL
                     VYRRTRKEMPARVEEVEHAEEEGIKFFFLHSPIRYEGDAQGRVCKAICERMELGEPDA
                     SGRRRPVPTGEYVDLEVDTVIVAIGNGPNPLIPRTTEGLDTKKWGNIKAEKATGRTSL
                     RGVWAGGDIVLGAATVILAMGAGRTAAKSIDDYLKTGDWPEVTLDG"
     gene            complement(4341..5672)
                     /locus_tag="PK710_12390"
     CDS             complement(4341..5672)
                     /locus_tag="PK710_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOT10566.1"
                     /translation="MAHLRLPLLRETFVILAFFLAACSGTVEKEEAQNDTGGSSGAGG
                     QAGSGGTSGSGGVAGSGGTSGSGGIAGSGGSSGSGGVAGSGGTSGSGGGDPITPLPGV
                     ATDITLEVYSDGWGTLLDTLTLATEGSIVIDVKEPNPYSDPPAYYIYAKAKGFYTELY
                     NCTKGQSIDVDLDAVPEEPNALAGVMFAKQSYFSDCYFANASVNLIDPSGVTRILQTD
                     SQGRYGIRELPLGDYKIAFDYQGMPFSFDLVNGPGTDYADLGFMEPMQAAAPNLYLYP
                     TSNTQVKVSLSFPQGGSVIESIPDYGTGWDVLVSPEGIIDNTFDYLFYEASLPPNVTT
                     SEGWMLDGAHLDASLRELLERVGFVGREVQDFLDYWVPILEGSPYYAVYPQDPEKLVT
                     LHISPAPEKILRALWFIRPLNHTINIVEPTPPEPFQRQGYVAAEWGVLRGI"
     gene            complement(5937..7193)
                     /locus_tag="PK710_12395"
     CDS             complement(5937..7193)
                     /locus_tag="PK710_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOT10567.1"
                     /translation="MACSRIRTAHEIAGILVVCLAACSGSISEDTPAGAGGASGSVGQ
                     AGSAGASGSSGASGLGGSSGTAGGEPIAPVPGVATDITLKVYSNGWGELLDTLTLATE
                     GSIVIDIDEQNPYSDPPSYDIYATAKGYYTELYHCNKGESIDVDLDAVPNEPNALAGV
                     IFAQQSYFADCYFDNATVNLIDPTGGSRTLQTDSQGRYGIRNMPLGDYTISFTYQGMP
                     FSFDLVNTAATDYADLAFKEPMQADAPNLYLYPTENTRLHVSLRFPQGGAIIDSVPDY
                     GNGWDVLVSPEGIIDDTFGYLFYEASLPPTVTTAQGWLLDGTRFEASMQELLERIGFV
                     GREVQDFLDYWIPILEGSRWYAVYPQDPDKLITLDISPAPDRILRSLLFIRPLNHKID
                     ILEPAPPEPFSREGYVAAEWGVLQGI"
     gene            complement(7258..>8440)
                     /locus_tag="PK710_12400"
     CDS             complement(7258..>8440)
                     /locus_tag="PK710_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10568.1"
                     /translation="MPPPTGATTLVTPAMTGQLQSLIFNASPHETFVFASGTYALEGA
                     YLWVRAPGVTLRSESGNPEDVVLDGGYQSSEIVTIAASDVTIAELSIRRAFTHPIHVT
                     GSDTGPTVNTRIYRVFITDPREQAIKINPSSNPVHFADDGEIACSRIVMTDAGRGHVS
                     DCYTGGVDAHQARGWLLRDNEIEGFWCESGLAEHAIHFWRGCRDTMVERNVLVDNARG
                     VGLGMATSGSARTYPDNPCPAAGGAYVDHYGGIVRNNFIVGKRPELFSSQDGFDTGIG
                     LWSACNAVAVHNTIVATNSLFSAIEWRFSTSTGIRILNNLATHALRERDGASAESQGN
                     IDNAALSQFEDVAAANLHLKAGSSAIDHGVSVGPGLCAEDIDGDLRDATPDVGADERL
                     P"
BASE COUNT         1901 a         2188 c         2588 g         1763 t
ORIGIN      
        1 cgaggaaacg ctctcggaat tcctcgacta cgccgtgagc attgaatccc gcaccgagat
       61 agcttccgaa aagagattcc aaccaaccca ggttggccaa gtcgctctgc aatgcgttgc
      121 gaatgccagg atgctgcacc ttcacggcaa cgtccgtacc gtcatgcaat cgggctcgat
      181 gcacttgccc aatggatgcg gaagctaccg gctgctcacc aaaagacgcg aatagttcgt
      241 gtgcataagc gccaagctcg gaccgcacga gactttcgat atgttctcga ggtgacggtg
      301 cggcgtgttg gaatagggat gccagcgctt ctccgtaggt gcttcgttgt gccgcgggaa
      361 gtacgccatc cacataaccg gccatttgac cgaacttggc tgctagacca cgcaaatcgc
      421 caaggactcg tgcggtatcg gccgcgctga gggactcgtt tgatcctcgc aattttcgcg
      481 ttcctaaccg agttcccgcg gccgcgagat gagcaaggcg ggacaagcgt ccccgtggca
      541 caagacgatc ggaactcacg atgatggatg gttcgaaggg gaggggaacg aggccaagtt
      601 ggatgtggta ttgcaggagc acatgctgtc tggagccgga cgttagttat tgagcaaggt
      661 agccagatgg taggctgcca gggtgtcgac gacatggtgt tgaggtgcgt gggaagggtc
      721 ttgtgcccac caccatagaa catgcagcag cattcccgcg agagcctggg cggctacgac
      781 gggatcgata tcctttcgaa cattaccgca agctgcgcct tcgcgaagca ggttttccat
      841 ggtcagggtc atgaagtcga cgacatcttt gcctacgggg gtgtgcaaga cttccggatg
      901 aaacatcact ttggataaat ctgggcggtt attgacaaaa tcgaccatca cctcgacatg
      961 gcgtcgaagc gagtggctgt aggatgggtg gcgttcttct gccaaagccc gcaggggagc
     1021 acgaagtttt ccaagtcctg tgatgaggat ttcgtggagc aaaccatcct tgtcctggaa
     1081 gtgcagatag agagtaccga cggctacgga agcagctcga gcgatatcga tggctttggt
     1141 tcgggatatg ccctgggtag cgaagaggcg aaggccctgg tcgagcaagc gttgatgggt
     1201 ttgcacctgg ctgcgttcgc gggcggacaa acgaagctgt ttcaaagaca tggggcaacc
     1261 ttcccgagcg ggttcgtgtt tctgaatcaa cgttgattca ctgaaccaac atatatcaac
     1321 gagcgctcat ggagaagggc ctacagattg ggagagaaac tgcggcgggt tggaaaagaa
     1381 gaccgtgatg ggggtgatga cggttctgtc gagcttttcc gacggtgtga catggtggag
     1441 gagcagcagc ctttgcgctg gggcgtggaa aggcgaagag ttcaggggag agaacgggga
     1501 ggatcttaca atttaatgtt ttaaaacaat agtttggatg aatcaaggac ggtggacggt
     1561 tgaccccttc taccgcgaaa ggggatcacc ttgccgcaca cggctcttga tcagggacgt
     1621 gggcaacgct atcaagagat ttgtcgagtg tccacgacac caccgtcgtg ggatcgaccc
     1681 accaaaccgg tgaaaggcga ttcatcgccg cgccgagatg caacccttgt agcaaggaac
     1741 ttactccatg accgcgaacc aagacgccgg ggaatccgtt ctggttgcca aggaatttct
     1801 atcttccaaa gtcaccaagt acgtagtgcg cgcccgggat atcgcccgtc gacgtcgccc
     1861 tggacagttc gtcatcgtgc gcagcttcgc cgagagcgag cgaattccgc tgacgattgc
     1921 cgatggggat gagcaagagg gaaccatcac gttgattgtc caggaggtgg gcaaatcaac
     1981 ggcagagatg gcgcatatga tgcgcgtggg agaccgtttt gccgatgtgc ttggtcctct
     2041 gggtatgccc acgcatatcg aaaaagtggg acgagtgatt gcggtgggag gcggcattgg
     2101 tatcgctccg ctgcatccca ttgtgcaagg cttccgcaaa gcggaaaacg aggtgatcgg
     2161 gatcatcggc gcgcgctccc gcgatctgct catcatggaa gaggaaatga aacgcgcttg
     2221 tcaccggctt cgtatcgtga ccgacgatgg gagttatggg gacaaaggtc ttgtgacgga
     2281 tgcgttgaga gcggaggtgg aagcctcggg caaaccagcg ctatgtgtcg ccattggccc
     2341 gctggtgatg atgcaagcgg tgtcgaaggt caccaaggag cttggcatca aaaccctggt
     2401 cagtttgaac ccggtcatgg tcgatggcac gggaatgtgt ggtggctgtc gcgtcacggt
     2461 ccacaacgaa accaagttcg tttgcgtgga tggaccggag ttcgatgccc acggcgtcga
     2521 cttcgacgag ctacgtcgtc ggcaagcttt ttatgcacct caggagcgag gggcctacga
     2581 tttgtttttg aaaaagcacc cgttcggcgc cgagcattgc aaagtgcata ggttggtaga
     2641 cgaggcgcaa cgagggaagg aagcgaaatg agcacggcac acgacaaggc ggttgtcact
     2701 ccgaagatcg atttgcccgc gggcgcagag cccaagcttt ccaacaagga gcgcatggcc
     2761 atcccgcgtc aggtcatgcc agaacaaagc gccgaagagc gaatctataa ctttctcgaa
     2821 gtgcccctgg gcttcgagga gaacgtcgct cgtatcgaag cacttcgttg catcaaatgc
     2881 aaaaagccaa cctgcatcga tggttgcccc gtaggcatca atatccccgg atttctcgag
     2941 aaaatcgcag aaggggattt tgaagggggc gcccgtgtcc tcaagcaaga caatatcctt
     3001 cccgccatct gcgggcgtgt ctgcccccag gaagagcaat gcgaaaagca gtgtatcgtc
     3061 ggaaaaaaga acgctcccgt ggcgatcgga cgtctcgagc gctttctcgc ggattgggaa
     3121 cgggagcaca acaaggtgca accgctcgtt cccggagaac ccacgggaaa gaaagtggcg
     3181 atcgtaggag ctggtccagc ggggctcacg gcagcagccg acctcgtgcg attcggccat
     3241 aaagtcgtcc tttacgaagc cctgcacaag cccggcggtg tgctcgtcta cggtatcccc
     3301 gaattccgct tgccgaaaaa aatcgtcgaa ttcgaggtgc agcaactcga aaaagccggc
     3361 gtcgaactgc ataacagtgt cgtcatcggc cagaccatca cgatgaccga actgcaagaa
     3421 acctacgacg ccatcttcgt cgccacgggc gctggtttgc cctatttcat gaaccttccg
     3481 ggtgaaaacc tgaacggcat ctactccgcc aacgagtacc tcacgcgagt caatctgatg
     3541 aaagcctatg accccgactc cgatacgccg gtggccaaaa gcaaaaatgt ggcggtcgtc
     3601 ggagggggga acgttgcgat ggacggggca agaacggcca aacgtctcgg agcggataac
     3661 gtctatctcg tgtatcgccg cacgcgcaaa gagatgcccg cccgcgtgga agaagtcgaa
     3721 cacgcggaag aggaggggat caagttcttc ttcctgcata gccccattcg ctatgaaggc
     3781 gatgctcagg ggcgcgtttg taaagcgatt tgcgagcgaa tggaactcgg cgagcccgat
     3841 gccagcggtc gtcgccgccc cgtgcccacc ggcgagtacg tggatctcga ggtggatacg
     3901 gtgatcgtag cgattggcaa cggcccgaat ccgttgattc cgcggaccac cgaagggctc
     3961 gacaccaaaa aatggggcaa tatcaaagcg gaaaaggcca ccggtcgcac gagccttcgg
     4021 ggcgtctggg ccggagggga tatcgtgctc ggggccgcca ccgtgatttt ggccatgggc
     4081 gctgggcgta cggcggccaa gtcgatcgat gattacctca aaaccggcga ttggcctgag
     4141 gtcaccctcg acggttgatt tcgcttatcg tatcgaaatg ggctggacgg ttctcggcta
     4201 gccccctgtc ccttgcctgg agatgagatg gaggcacaac attagaggtg tgccgaggct
     4261 gaagacgaac taaggtatat tgttgtaatt acgtaggaaa agatcatacg ttgcgcagtg
     4321 ttgaccatgc tggggatgga tcaaataccg cgcagcacgc cccattccgc ggctacatat
     4381 ccttgacgtt ggaaaggttc gggcggggtg ggttcgacga tattgatggt gtggttcagg
     4441 ggtcggatga accatagggc gcgcaggatt ttttctggcg ctggggagat atgcagcgtc
     4501 acgagttttt ccggatcttg gggatagacg gcataatagg gagagccttc gaggatggga
     4561 acccaatagt cgaggaagtc ctgaacttct cttccgacga aaccgacgcg ttccagcagt
     4621 tctcgcaggg aggcgtcgag gtgggcgcca tcgagcatcc aaccttcgga agtggtgaca
     4681 ttgggcggga ggcttgcttc gtagaaaagg taatcaaagg tgttatcgat gattccttcc
     4741 ggggaaacca gcacatccca gcccgtaccg tagtcgggga tggattcgat gacggaacct
     4801 ccttggggga agctcaggga gactttcact tgcgtgttcg aggtggggta taggtagaga
     4861 ttgggggcag cggcttgcat gggttccatg aaccccaggt cggcgtaatc cgtacccggg
     4921 ccattcacca ggtcgaaaga aaagggcatc ccttggtaat caaaggcgat tttgtagtct
     4981 cccaggggca gctcccggat tccatagcga ccctggctat cggtttgcaa aatcctggtg
     5041 acgcccgagg ggtcgatgag gttcaccgag gcgttggcga agtagcaatc cgaaaaatag
     5101 gattgtttgg caaacatcac cccggcgagg gcattgggtt cctcaggaac ggcatcgagg
     5161 tccacatcga tgctctggcc tttggtgcaa ttatatagtt ccgtatagaa gcctttggct
     5221 ttggcataga tataataggc ggggggatcc gaataggggt ttggctcttt gacatcgatg
     5281 acaatgctgc cttcggtggc gagggtgagg gtatcgagca gcgttcccca gccatccgaa
     5341 tagacttcca gggtgatatc ggtggccact cctggaagcg gtgtgatggg atctccgcca
     5401 cctgacccgc tggtgccgcc cgagcctgcg acgcctcctg aaccgctgct accgcccgag
     5461 cctgcgatgc cacctgaccc gctggtgccg cccgagcctg cgacgcctcc tgaaccgctt
     5521 gtccctccgc ttcccgcttg accgccggct cctgatgagc ctcctgtgtc gttctgtgcc
     5581 tcttcctttt ctacggttcc ggaacaggcc gcaagaaaaa acgcaaggat tacaaatgtt
     5641 tctcgcaata ggggtagacg aaggtgtgcc atggctattc ctcagaggtg ttctatgagc
     5701 agttgtaaag caaatgtgga gccaacttgt atgaatgctc gaggttcatc gtggtgacga
     5761 agcgttccga acgctgcccg cgggcttggt attgagccgg aaacatcgtg ccattccagt
     5821 tcgcggtgga gtaggaaata cgcaatgtca agcgtttgtg cgattgcgct ccctgaaaaa
     5881 ctctcgattg acgatgctca atcacaagat gttgcctatt tttgagtcga attggttcaa
     5941 atgccctgaa gaacacccca ttccgcggcg acataacctt ctcttgaaaa tggttcggga
     6001 ggggctggct cgaggatatc gatcttgtgg ttcaaaggtc gtatgaacag caaggagcgg
     6061 aggattctgt ccggagcagg agaaatatcc agcgtgatga gcttgtccgg gtcttgggga
     6121 tagaccgcgt accatcggga gccttcgaga atcgggatcc aataatcgag gaagtcctgc
     6181 acctcccttc cgacaaagcc gattcgttcc aacaattctt gcatcgaggc ttcgaatcgg
     6241 gtgccatcga gcagccaccc ttgggcggtg gtcaccgtgg ggggaagact tgcctcgtaa
     6301 aaaaggtaac cgaaggtgtc atcgatgatt ccctccgggg aaacaagaac atcccaaccg
     6361 ttgccgtaat caggaaccga gtcgatgatg gcgccacctt ggggaaaacg caacgacacg
     6421 tggagccggg tgttttcggt cggataaagg tataggttgg gcgcatcggc ttgcatgggt
     6481 tccttgaaag ccaggtcggc ataatccgtg gcagccgtat tcacgaggtc gaaggaaaag
     6541 ggcatcccct ggtacgtaaa agagatcgta tagtcgccaa gcggcatgtt tcggatgcca
     6601 taccggcctt ggctatcggt ttgtagcgtc cgggaacccc ccgtggggtc gatgaggttc
     6661 accgtagcgt tatcgaagta acaatcggcg aagtagcttt gttgagcaaa tatcactcct
     6721 gcgagcgcat tgggttcgtt ggggacagca tcgagatcca catcgatgct ctcgcccttg
     6781 ttgcaatgat acaactcggt ataatagcct ttggctgttg cgtagatatc ataagaggga
     6841 ggatccgaat aagggttttg ttcgtcaata tcgatgacaa tgctgccttc ggtcgcgagg
     6901 gtgagcgtat cgagcagctc tccccatccg ttggaataga cttttaacgt gatgtccgtg
     6961 gcgacccccg gtacgggggc gatgggctca ccgccagccg tgccgctgct cccacctaac
     7021 ccactggcac cgctggaacc acttgctcca gcgcttcctg cctggcccac gcttcccgag
     7081 gcgccaccgg caccagcagg agtgtcttcc gatatcgaac ctgagcaagc tgcaagacat
     7141 acaactagga tcccagcgat ttcgtgggct gtgcggatac gtgaacaggc catggtaacc
     7201 tccccggaag caatcaccgg acaaactgta aaagtgtaca cgatgccttt gcttcggtca
     7261 aggcaagcgt tcgtcggcgc ctacatcagg tgttgcgtct cgtaagtctc cgtcgatatc
     7321 ttccgcgcac agaccaggac ccacggatac cccgtggtcg atggcggagg agcccgcttt
     7381 gagatgcaag ttggcagcgg cgacatcctc gaattgtgaa agagccgcat tgtcgatatt
     7441 tccttgcgat tcagcggagg caccgtcgcg ctctcgcagc gcatgggtgg cgagattgtt
     7501 gaggatacga atcccggtag acgtggaaaa gcgccattca atcgcggaaa ataggctgtt
     7561 tgtagccacg atggtgttgt ggacggccac cgcgttgcag gcggaccaga gcccgattcc
     7621 cgtatcgaaa ccgtcttgcg atgagaataa ctcggggcgt tttcctacga tgaagttatt
     7681 tcgaacgatc cctccgtaat ggtcgacata ggctccgccc gcggcggggc aaggattgtc
     7741 cgggtaggtt cgagcagagc cggaggtggc catcccaagt cccactcccc gggcgttgtc
     7801 gaccaataca ttgcgctcca ccatggtatc ccggcatccg cgccaaaagt ggatggcgtg
     7861 ttccgccaag ccggattcgc accaaaagcc ctcgatttca ttgtcccgaa gaagccatcc
     7921 ccgggcctga tgggcatcga ctcccccggt atagcagtcg ctaacgtgcc ctcttcccgc
     7981 atcggtcatg acgatccggc tgcacgcgat ctcgccgtcg tcagcgaaat ggaccggatt
     8041 cgagctggga ttgattttga tggcctgttc gcgagggtct gtgatgaata cgcgataaat
     8101 gcgtgtgttg accgtagggc cggtgtccga accggtaaca tgaatggggt gggtaaaggc
     8161 acggcgaatg gacaattcgg cgatggtcac gtcggaagca gcaatggtga cgatttcgct
     8221 cgattgataa ccgccatcga gtacgacatc ttcaggattt ccgctttcag atcgcagggt
     8281 tacgcctgga gcgcggaccc ataggtaggc gccttcgagg gcataggttc cgctcgcaaa
     8341 cacgaaagtc tcatgagggg aggcattgaa aatgagtgat tggagctggc ccgtcatggc
     8401 cggggtcacg agggtcgttg ctccggtggg aggaggcatc
//
LOCUS       DAOSOR010000209         8399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8417_length_8399_cov_5.047459, whole genome shotgun sequence.
ACCESSION   DAOSOR010000209 DAOSOR010000000
VERSION     DAOSOR010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8399
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8417_length_8399_cov_5.047459"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1491)
                     /locus_tag="PK710_12405"
     CDS             complement(<1..1491)
                     /locus_tag="PK710_12405"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOT10569.1"
                     /translation="MREPAPSFFAKMRSHATSLLLVVIATALGLYVWIDRDQPSTTQI
                     EARKGNLLPIFRQDDLREIVIEQGPHKITLARRETADAGEATYDLIEQDGKPQDAVTA
                     DQFAVDKLVHALQDASPLRHVEGQDLKSLGLDPPLHMLTVNLGSVVYRIGIGDEAKSP
                     KNARFVSLEGQGAYVLSPASAQDFMRPIDSYRTRRIVPVGVTELAAIEVAGSQGTLRL
                     ARGSWGGFRVESEPAKPRASSLRLDRIYRAFADLNAEHFVDPKLASRIVDGAERKIRL
                     SLTPKSGPRVELVLAGACPIHNDTSVDASTDNPPSAAKLVVAIRTAPTPVHACVPSSV
                     FDDLDLAPASVVDRRVFRGTADEVEELRIVQGDRELELARAGTGWHERKPQDSELADE
                     DVAGYLAALLTVEGTIAIEADEKKLGLSPPHATIRLQHPSLDSLDIPPQQVEIGGRVT
                     TQEGASIAVRRREDGAILLVPASTAPLFEPSTAHMRSTALLQVGAQQ"
     gene            complement(1488..3113)
                     /locus_tag="PK710_12410"
     CDS             complement(1488..3113)
                     /locus_tag="PK710_12410"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HOT10570.1"
                     /translation="MSDTSRNHRSDDGVVTRSTKAVSALGVLAAMAIALLVNIVSARH
                     YARWDFTTAKLYTLSHATLSTVRGLEQNVDVQVLLSASDPLYGSVKNLLEVIRSNTTR
                     LSVTFVDPDRYPAEFVAIQQKYDIVAGRSEDGRVVTDAAIIVASRDRHWFITAEDLVD
                     FSEVDDGRTKSKLEQSITGGIRAVLGGKRSRICFTSGHGEYSLDDSSGQGLGEFRDRL
                     QKNNYDAETIDTTRSDPAKALEACDAVVVAGPSTAFSQTESDALMARMRAGMSGFFLL
                     NPIFDTERKTQLPTGLESVTRLFGIGLANDFVFELDDDSRVPRGTGEIFFPSLEAHAT
                     TEGLVGLSMAVTGLRVVAIRSRSLDVVGDGVRPVTILKTSERAFGMKNFHAWAEKGGE
                     PTPTVDDRRGPLAIGLAAELANAPDSSQTHGARLVVLGTANLVLGQNWRDMALRGNAL
                     LAGNIMSWVAARPPIVDIPAKITPAATLRITEASLQEIVRYVLLFMPGAALLIGVAVY
                     LRRRSRDDRSDHGEKKKGSLSHDDSSSENEENR"
     gene            complement(3110..3856)
                     /locus_tag="PK710_12415"
     CDS             complement(3110..3856)
                     /locus_tag="PK710_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT10571.1"
                     /translation="MTGFWPIFKRDLFALFVTPMAWALMVIFLLLEGLNFYWLTVHFA
                     NQVDLAVDHGPLQWFFGETVLFYLPLLMLPPALTMRSFAEERRAGTIESLLTAPVSTP
                     GIVLAKWCASFVTYLVAWAPTVLYIVILRRAGEVDWRVVASSYLGVILIGASFLAIGV
                     LMSAMSKSQFVAVLLSLAVTVGLFILGIGEFVFDRGLAHDICSHVSIWSQMADFSKGQ
                     IDSRYVVFHLSLIVLPLFICVRVVDSWRWG"
     gene            complement(3853..4857)
                     /locus_tag="PK710_12420"
     CDS             complement(3853..4857)
                     /locus_tag="PK710_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT10572.1"
                     /translation="MIEVKHLTKSYGSVQAVHDVSFTVGDKEVVGFLGPNGAGKTTTL
                     RIIAGFLGATRGRVTVDGIDTVRDPVRARYRIGYMPENVPLYPEMRVGEYLQFRAELK
                     GVPRARRKQAVERAMDDVRIHDHAQVLIGNLSKGYRQRVGLADALVARPSVLVLDEPT
                     AGLDPNQIREVRELIRTVAEQHAVLLSTHIMSEVEATCDRALVIDRGRLVAQGTLDAL
                     RTLRRSGATRFVVRGDGARAVEVLKNTGEIHDVRDVRRIADAVVLEARWAAMADPTRE
                     TEQAVHRLVAADLKLQEVSPVKTSLEDVFALLTEEEPRESDPSVEVEDSAASREEVEP
                     "
     gene            complement(4891..6915)
                     /locus_tag="PK710_12425"
     CDS             complement(4891..6915)
                     /locus_tag="PK710_12425"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOT10573.1"
                     /translation="MAAPSDPSSRSPRMASQDVPRRDRDQNTHGLQEIDAEQLRASVI
                     DGEKRDHGTLDSDLESAATIESVPNSKSSADTSSTPLDSPEIGDSHDQPNPGSPQKPV
                     VHMGLVVDPDVADTDDVPLDQLSSEPPPIVRQRRRRSIPEQPSLRPHTAQALPSTSEL
                     LDPVAVPPPIPRQSPRVAAPTVLSPRMTAVFGVLFGLAGIVAVFAALNRVSPQSANAS
                     PRAKPEEVASATPRLTATRALDQLPDFEEDGPPIPGPWRVASLADDANLRTIKGVVGL
                     EPFVTTLQAKGVSKAETYRILAAFKKHDAFKRLRKADQFTVVLDRSTGKVVGFELEIS
                     ALEVYQAKPNEEGLLVGTRLDMKVGSRPRVAALRISEGDLASNLKRGRLRESVTAIID
                     RAFDGRASVASMPTGSTLRVILQEKTALGRFAEYEYVEALEYVSSKPDTKPLRLYRFK
                     DGGRYGYFNQDGKEPYRGGWRMPCPGAPITSPFNPKRLHPVLKTIRPHQGTDFGAPTG
                     TPIHATSFGTVDWVGPRGPAGNLVILKHPGNIDSYYMHMSRFAPGLKKGDKVETFQVI
                     GFVGSTGRSTGPHLHFGIKKNDVWIDPMSLQLDGDRLVSASLRSDFEKVKAELDKRLD
                     AIELPAELAPTSAPEPKVDPSEDPALPRENYDDDELEPGTVHDEPYDDPQ"
     gene            7083..7811
                     /locus_tag="PK710_12430"
     CDS             7083..7811
                     /locus_tag="PK710_12430"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT10574.1"
                     /translation="MDERLKQQLAIGREYYQKREFDKAEPILREVLEQYERFADVHNM
                     LGVIAHERGDFAQAEKHLERATELNPHYTEALLNLAVTYNDQSKYEAGRSVYKRIRAT
                     RERSADNLDPFVRGKIANMHAELAQAYEDAGLRSDAIRELERAVSMCPTFADLQTRLG
                     TLHRDSGDLEQARFHYEAARNANPRYPLARLMLGVTLLALNEVEAAKAEWRAVLEHDP
                     GNKSATMYLRMAENIPRTSQPPDI"
     gene            8169..>8399
                     /locus_tag="PK710_12435"
     CDS             8169..>8399
                     /locus_tag="PK710_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10575.1"
                     /translation="MNTHTHHAATSEPVVSPFVSSNPFETDDSNNATTYVLTKSGPDI
                     DAKEFERPDVVAIEIMIQWGENVLHIEHLSPPR"
BASE COUNT         1895 a         2466 c         2347 g         1691 t
ORIGIN      
        1 ctgctgtgct cccacctgta acaatgccgt ggatcgcatg tgtgccgtgg acggctcaaa
       61 cagcggcgcg gtggaagcgg gaaccaacag gatcgcaccg tcttcgcgtc gacgaaccgc
      121 gatcgatgca ccttcttgcg tcgtgactcg tccgccgatc tcgacctgtt gaggcggaat
      181 atcgagtgaa tccagcgacg gatgctgcaa gcggatggtt gcgtggggag gggacaagcc
      241 aagttttttc tcgtcggctt cgatggcgat agttccctcc acggtgagca gggccgccag
      301 gtatccagcg acatcctcat cggcaagctc gctatcttgc ggcttgcgtt catgccagcc
      361 ggttcccgcg cgcgcaagct ccagttcgcg atctccctgc acgattcgta gctcttctac
      421 ctcatcggcc gtaccgcgaa acacgcgtcg atcgacgacc gatgcaggtg ccaagtccag
      481 atcgtcgaac acggacgatg gcacacaggc atgaacgggt gtaggggcag tgcggattgc
      541 cactacgagt tttgcggcgg atggaggatt gtcggtggaa gcgtcgacgg acgtatcgtt
      601 gtggatgggg cacgcaccag ccagcaccaa ttcgacgcga ggacccgatt tcggagtcaa
      661 cgacaggcga atttttcgct ccgccccgtc gacgatccga gaggcgagct tgggatcgac
      721 gaaatgctcg gcgttcaagt ccgcgaaagc acgatagatt ctgtcgagac ggagggatga
      781 agcgcgaggt ttggcaggct cgctttccac ccgaaaacca ccccaagacc cacgggccag
      841 acgcagggtg ccttggctgc cggctacttc gatcgctgcc aattccgtca ccccaaccgg
      901 cacgatgcgc cgcgttcgat agctgtcgat cggacgcatg aagtcctggg ccgacgcggg
      961 ggataataca taagcgccct gcccttcaag actaacgaac cgcgcatttt tcggtgactt
     1021 ggcttcatcc cctatcccga tccggtagac gacgcttccc aggttcacgg tcaacatgtg
     1081 aaggggcgga tcgagtccca aggacttcaa gtcctgtccc tccacgtgtc gaagcggtga
     1141 tgcgtcttgc agggcatgaa ccagcttgtc taccgcgaat tgatccgcag tcaccgcgtc
     1201 ttgcggcttg ccatcctgct cgatgagatc gtaggtcgcc tcgcccgcat cggccgtctc
     1261 acgacgcgcg agggtgattt tgtgaggccc ttgttcgatc acgatctccc gcagatcgtc
     1321 ttggcgaaag atgggtaaaa gattgccctt tctcgcttcg atttgcgtgg tggagggctg
     1381 gtctcgatcg atccatacat acaggccgag cgccgtggcg atgaccacca gaagaagaga
     1441 ggtggcatgg gaacgcatct tggcgaaaaa cgaaggcgca ggttctctca tcgattttcc
     1501 tcgttctcgc tggaagaatc gtcgtgagac aaagagcctt tcttcttttc accatggtcc
     1561 gatcgatcat cgcgagaacg tcgtcgcaag tacacggcaa cgccgatcaa caaggccgcg
     1621 ccgggcatga aaagcagcac atagcgaacg atttcctgca gagaggcttc cgtgatccgc
     1681 agggttgccg ccggagtgat ttttgcggga atgtcgacga tcggcgggcg cgcagctacc
     1741 caggacatga tatttcccgc aagcaaagcg tttcctcgca gcgccatatc acgccaattc
     1801 tgaccgagca cgagattggc cgttcccagc accaccaaac gcgcgccatg ggtctgagaa
     1861 gaatccggag cgttggctag ttccgcggca agaccgatcg ccaacggccc ccgtcgatca
     1921 tccacggtcg gcgtgggttc cccgcctttt tcggcccacg cgtgaaagtt tttcatgcca
     1981 aacgcgcgtt cgctcgtttt caggatcgtc accggccgca ccccatcgcc aaccacatcc
     2041 aaagaacgcg atcggatggc taccacccgc aaacccgtca cggccatgga caatccaacg
     2101 agtccttctg tcgtggcatg ggcttcgagc gatgggaaaa agatttctcc cgtgccccga
     2161 ggcacgcgag aatcatcatc caactcgaat acgaaatcgt tggcgagccc gataccaaat
     2221 agccgcgtta ccgactccaa ccctgtcggc aactgcgttt ttctttccgt gtcgaaaatg
     2281 ggattgagga ggaaaaaccc gctcattccg gcccgcattc gcgccatcag ggcatcgctc
     2341 tccgtttgcg aaaatgccgt gctcggtcct gctaccacca cggcatcaca cgcttccaac
     2401 gccttggccg gatcgcttcg cgtggtatca atggtttctg catcataatt atttttctga
     2461 agccggtctc gaaactcccc aagaccctga ccactgctgt catcgagtga atactcccca
     2521 tggcccgagg tgaagcaaat gcgacttcgc ttgccaccca acacggcacg gattcccccc
     2581 gtgatggatt gctcgagttt cgacttggtc cgcccgtcat ccacttcgga aaaatccacc
     2641 agatcctcgg ccgtaatgaa ccaatgacga tcccgagagg ctacgatgat ggccgcatcg
     2701 gtaacgacac gaccatcctc cgaacgtccg gccacgatgt cgtacttttg ctgaatcgca
     2761 acaaattcag caggatagcg atcaggatcc acgaaggtga ccgacaggcg tgtcgtgttc
     2821 gagcgaatga cctccaacaa gttcttcacc gacccataca gcgggtcgga agcggacagc
     2881 agaacctgaa catcgacgtt ttgctcgaga ccacgaaccg tcgaaagggt tgcgtgacta
     2941 agcgtgtaca gcttcgcggt ggtgaagtcc catcgcgcat aatgccttgc ggaaacgatg
     3001 ttgaccaaca gagcgatcgc catggccgca agcacaccca aagccgatac ggctttcgtc
     3061 gatcgagtca ccacgccgtc gtcagaacgg tgattgcgcg aagtgtcgct catccccacc
     3121 tccaagaatc caccacccgg acacaaatga acaggggtag gacaatgagc gagaggtgaa
     3181 atacgacgta acgggaatcg atctgacctt tggaaaagtc cgccatttgc gaccaaatcg
     3241 aaacgtgcga gcagatgtca tgggcgaggc cgcgatcgaa aacgaactca ccaatgccaa
     3301 ggatgaaaag ccccacggtg acggcgagag acagaaggac cgccacgaat tggcttttgc
     3361 tcatcgcgct catgagcacg ccgatggcta gaaaagaagc tccaatgagg atgaccccca
     3421 ggtacgaaga ggcaacgacg cgccaatcga cttccccggc gcgtcgcaga atgacgatat
     3481 aaagaacagt aggagcccag gctaccaagt aagtcacgaa cgaggcacac cacttggcaa
     3541 ggacgatacc tggtgtagag accggggctg tgagcaagga ttcgatggtt ccggcgcgtc
     3601 gctcctcggc gaaagagcgc atggtgagag cgggcggaag catgagcaac ggaagataaa
     3661 acaggacggt ttcaccgaaa aaccattgta gggggccatg gtcgacggca aggtcgacct
     3721 ggttggcgaa gtggacggtc aaccaataga agttgagtcc ttcgagcaac aggaagatga
     3781 ccatcaatgc ccaggccatg ggcgtgacga aaagtgcgaa caagtcgcgt ttgaagatgg
     3841 gccaaaagcc ggtcacggtt ccacctcttc gcgggatgcg gcggaatcct cgacctcgac
     3901 ggatgggtct gactctcgag gttcttcctc cgtaagcaag gcaaatacgt cttcgagcga
     3961 ggttttcacg ggggatactt cttgcaactt caagtcggcc gcgacaaggc gatgcacggc
     4021 ttgctcggtt tcacgagttg ggtctgccat tgccgcccat cgcgcttcga gaacgacagc
     4081 gtccgcgata cgcctcacgt cgcgcacatc gtgaatttca cctgtatttt tgagtacttc
     4141 cactgcgcgg gcaccatccc cacgaacgac gaatcgcgtg gcccccgaac ggcgaagggt
     4201 tcgaagtgca tccagtgtcc cttgcgccac gagacgccca cggtcgatga ccaacgcgcg
     4261 gtcgcaagtc gcttccacct ccgacatgat atgcgtcgaa agcaaaaccg catgctgctc
     4321 ggctaccgtt cgaatgagtt cgcgcacttc gcgaatctga ttgggatcga gccctgccgt
     4381 cggttcatcg agaaccaaca cggaaggacg agctaccaag gcatccgcca agccaacccg
     4441 ttgccgatac cctttggaaa ggttaccgat gagcacctga gcgtgatcat ggatgcgaac
     4501 gtcatccatg gcgcgttcga ccgcttgctt gcgtcgcgct cgaggcactc ctttgagttc
     4561 cgcgcgaaat tgcaagtact cccccacgcg catttcagga tacaagggga cattttcggg
     4621 catgtacccg atgcgataac gcgcgcgcac cgggtcccga accgtatcga tgccatccac
     4681 cgtcaccctg ccgcgggtag ctcccaaaaa accggcaatg atgcgcaacg tggtggtttt
     4741 tcccgctccg tttgggccaa gaaatcccac cacctccttg tccccgaccg taaaagagac
     4801 atcgtggacg gcttgcacgc tgccataact cttggtcaag tgtttgactt cgatcatcgg
     4861 tgactcaggt cataccacga ccccccacgc tcattgcgga tcgtcatacg gctcgtcgtg
     4921 aacggtccca ggctcgagtt catcgtcgtc atagttctca cggggcaaag caggatcctc
     4981 ggagggatcc actttgggtt ccggtgccga cgtcggcgcc aactccgcag gaagctcgat
     5041 ggcatcaagc cgcttgtcca actccgcttt cactttctcg aaatcgcttc gcaacgaagc
     5101 actcaccaga cggtcaccgt cgagttgcaa cgacatgggg tcgatccaaa cgtcattttt
     5161 cttgattccg aaatgaagat ggggaccggt ggatcgtccc gtcgacccta cgaaaccgat
     5221 gacttgaaag gtttctactt tgtcaccttt cttcaagccc ggtgcgaagc ggctcatgtg
     5281 catataataa gagtcgatat ttccggggtg tttcaggatc acgaggttgc cggcgggccc
     5341 ccggggacca acccaatcca ccgttccaaa cgatgtcgca tgaatcggag tcccggtcgg
     5401 agcaccaaag tccgtgcctt gatgcggacg aatcgttttg agtacgggat gcagtcgttt
     5461 cggattgaaa ggcgatgtaa tgggtgcgcc aggacacggc attcgccacc caccgcgata
     5521 aggctccttg ccatcctggt tgaagtaccc ataacgccca ccatctttga aacggtataa
     5581 acgtaagggt tttgtatctg gtttcgagga gacatactcg agggcctcga catattcgta
     5641 ttccgcaaat cgaccgagag ccgtcttctc ttgcaaaatc acacgcaacg tggatccggt
     5701 cggcatgctc gccacgctcg cacgaccatc gaaagcccgg tcgatgatgg ccgtcaccga
     5761 ttcacgcagc cgtccccgtt tcaggttcga agcgagatcc ccttccgaaa tccgtagcgc
     5821 ggccactcgt ggtcgtgacc ccactttcat atcgagccgc gtgcctacca gcaaaccctc
     5881 ctcgttgggc ttggcctgat acacttccaa ggcgctgatc tcgagttcga atcccacgac
     5941 cttgcccgtc gaacgatcca atacgaccgt aaactggtcg gcttttctca gacgcttgaa
     6001 cgcatcatgc tttttgaatg ccgcaagaat tcgataggtt tccgctttgc tcaccccttt
     6061 ggcttgcagt gtcgtcacga atggctccaa tcccaccaca cccttgatcg ttcgcagatt
     6121 ggcatcgtcc gcgagcgaag cgacgcgcca agggccggga ataggcggac cgtcttcttc
     6181 gaagtccggc aactgatcca gcgcccgcgt ggccgtcaac cttggagtcg cggaggcgac
     6241 ctcttcaggc ttcgccctcg gactggcatt ggccgactgc ggactcacac ggttgagcgc
     6301 tgcaaaaacc gccacaattc ccgccaaccc gaacaaaaca ccaaataccg cggtcattcg
     6361 cggtgataaa accgtcggcg ccgcaacccg tggcgattgt cgaggaatcg gtggcggtac
     6421 cgccacggga tccaacaact ccgacgtcga agggagcgct tgggccgtat gaggccggag
     6481 agacggctgc tccgggatag accgacgtcg gcgctgtcgc acaatcgggg gaggctcgga
     6541 actaagctga tctaaaggca catcgtcggt atccgccacg tccggatcga ccaccaaacc
     6601 catgtgaaca acgggttttt gcggagatcc aggattcggc tggtcgtggc tatcgccgat
     6661 ttccggtgag tccaagggtg tcgaagacgt gtcggccgag gatttcgaat tggggacgga
     6721 ttcgatagtt gctgccgatt cgagatccga atcgagggtt ccatgatcac gcttctcgcc
     6781 gtcgatgacg gaggcacgaa gctgttcggc atcgatttcc tgcaaaccat gagtgttctg
     6841 atcgcggtcc ctgcgcggaa cgtcttggct tgccattcgc ggagagcggg aagaggggtc
     6901 ggagggagct gccacgccca cgggttacca ggcgacggga aagggagcaa ccaaggttgt
     6961 tggcaagatt catcgggtgc ggtcgcgttc cagtgtcatc aaaatgccac aattatgctt
     7021 tacttggggt tttctaagct ggaaggggtg acacggggcc cggaataggc gaatcatgag
     7081 gcatggacga gcgcctgaag cagcagttgg ccatcggtcg cgagtattat caaaaacgcg
     7141 agttcgacaa ggcggaaccg atccttcggg aagtgttgga gcagtacgag aggttcgccg
     7201 acgttcataa catgctgggt gtgatcgcgc atgagcgagg ggacttcgcg caagcggaga
     7261 agcatttgga gcgggcgacg gaactcaatc cgcattacac ggaagcgtta ttgaatcttg
     7321 cggtgacgta taacgatcag tcgaagtatg aagcgggtcg gtccgtgtac aagaggattc
     7381 gcgcgacgcg agaacgcagt gcggacaact tggatccatt cgtacgcggc aagatagcga
     7441 atatgcatgc ggagcttgcg caagcttatg aggatgcggg actgcgcagc gacgccattc
     7501 gggagttgga gcgggcggta tcgatgtgcc caacctttgc tgatttgcaa acacgattgg
     7561 gtacactgca tcgtgattct ggggacctgg agcaagccag gtttcattat gaagccgcgc
     7621 gcaacgcgaa tcctcgctat cctctagctc gtttgatgct cggtgtgacc ctgctcgcgc
     7681 tcaacgaagt ggaagccgca aaagccgagt ggcgcgccgt tctcgaacat gatccgggaa
     7741 ataaaagcgc gaccatgtat ctacggatgg cagaaaacat cccccgaacg tctcaaccgc
     7801 ccgacatctg attgcttcca cgtatcgatc gtcgaaaacg cgcatcccaa tcatctcatg
     7861 acggtgcgtc gacgatcacc gcacgcaaat atcgaatctc gcttccccac gaatcgcctt
     7921 cgatatctgc cctccctgta tcttcacaac gtcgatgcat tccatcggat tgcgcatatt
     7981 ttttttgcgc ctgggaactc ttgcctccga atcgatgtcg ttcgcatcag ttcgctcaag
     8041 agggcacccc gcgcagtcac ccatcgatac ggtgacgtga gtttggaaga actacacacc
     8101 ggcaagacgc gcgagtcctc cgagcaaaga caccgaccca cttttacgtg acgagtcgag
     8161 gaggcaccat gaatacgcac acccatcatg cagctacatc cgaaccggtt gtctcccctt
     8221 tcgtgtcgag caaccctttc gaaaccgacg attcgaacaa tgcaacgacc tatgttctaa
     8281 ccaagtccgg gcccgatatc gatgccaagg aattcgaacg acccgacgtt gtcgccatcg
     8341 agatcatgat tcaatggggg gaaaacgttc ttcatatcga gcatctatcg ccgcctcgc
//
LOCUS       DAOSOR010000210         8343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8484_length_8343_cov_4.630550, whole genome shotgun sequence.
ACCESSION   DAOSOR010000210 DAOSOR010000000
VERSION     DAOSOR010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8343
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8484_length_8343_cov_4.630550"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PK710_12440"
     CDS             complement(<1..260)
                     /locus_tag="PK710_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10576.1"
                     /translation="MWTWIRKRWRKNAWAPEQLSSWRINDARLGLPPRAVKSENPPYI
                     PRDWHRAGWHELLFWASVFNLPLAPILAVLAGFEGDALLAVIL"
     gene            350..1216
                     /locus_tag="PK710_12445"
     CDS             350..1216
                     /locus_tag="PK710_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10577.1"
                     /translation="MSEPLHDSKWNVFTALDAFSLGSPMLLCRGKMMENLRLIRLMMV
                     ACLASCGRETEKKTEPPPVPNAVAPTVAVSSAKPAHVEPVRIETVPVAGDLPVFVLRG
                     ATAARRMVFLHGHCSHALGFLQSFPHAAARHGTIVAVQADKACADENYREWTDTPERL
                     DRRIEAAFRTLGSSDSLDGLAILGYSSGAVLAEMLAHRSPKRYSNVVLIGGPQKPVEW
                     RLRRTRSTAMVAGERDRQDLMQMGARELQLSGIPSKFFILPGAKHGQMGPQAEAVMEQ
                     VLTWLWDNEREI"
     gene            complement(1217..2893)
                     /locus_tag="PK710_12450"
     CDS             complement(1217..2893)
                     /locus_tag="PK710_12450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOT10578.1"
                     /translation="MKKLFQLPSDIGWRDQILGATMAILVTAWLLSTADAIGFARDEG
                     FYFRAATQYAEWFNLLVSDPSRAFSRATIDRIWGYNHEHPSLIKSLFSLSWLAFHEKW
                     KVFSHASDAFRLPAMALSGVAVWVTYLFGARVYSRTAGLIAACLFLLTPRVFYHAHLA
                     CFDLPIVALWTLCIYIYWRSIEKGGLGWALLAGVVYGLTLDTKHNAWILPAVFLPHAV
                     LVVHRHLRRDWKDQILRVPTSLLAMAIVGPLVFWALWPWMWHDTAARLREYFDFHFNH
                     VYYNMEFLGTNYFDAPSPRAYMPLMIAATVPSITLLLCAVGAGQKVRGALGWLRAWKQ
                     NTLTEPERPTYPDMLFALAIIAAISPWFFAKTPIFGGTKHWMTAYPFMALFAAHGLEL
                     SLRALSSVLTRWSTRWVAVAKTAVVAFVLAGPLAITMHAHPFGLASYMPWIGGTKGGA
                     SLGLNRQFWGYTSQNASEQFLAEHAKQGATVFIHDTAWDSWARMIDEGRIRSDLRGVG
                     APSQGDYALVQHELHMNEVEFQMWVAFGTRAPVYIVTHDDVPIVSIYQRP"
     gene            complement(2890..3804)
                     /locus_tag="PK710_12455"
     CDS             complement(2890..3804)
                     /locus_tag="PK710_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10579.1"
                     /translation="MAWIQRWWFVPVLLVAPLELGLHISQTRPVLKPEDWTAVRSQLE
                     AQVQPSDLVVVSPSWLEPLGRLQLGDGLMTVQRVARADESRFPVAIEVSLGSARHPAL
                     ASWHLEQEQSWGALQMRRLRNPDPHPVIDDLVSHATAHQMDVSLVRGEAVQPCEWRHG
                     QPVTGPLGFGPAQPSDRYHCRNTWVGETINADLEYKPRRCIHAPPPGGQEVLRLRWKS
                     VRFGTHLLGHHALYVEAERDRKGPAVRIVFSSDDETLGEAIHEDGQGWSSFAFDTTSR
                     AGKTAELQAEISSTRADRRTYCFEVTTR"
     gene            4200..4568
                     /gene="hypA"
                     /locus_tag="PK710_12460"
     CDS             4200..4568
                     /gene="hypA"
                     /locus_tag="PK710_12460"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOT10580.1"
                     /translation="MGSGFRPRAVHELSIAQSILDTVLEQADKHGAVRVLRIHLRIGQ
                     LTAVVQEALDMAFEVLTRDTIAAHARITVERVPWRVRCSRCEHEYSVQNDLPTCSRCG
                     HLGGETLGGRELQIVEMDIE"
     gene            4565..5224
                     /gene="hypB"
                     /locus_tag="PK710_12465"
     CDS             4565..5224
                     /gene="hypB"
                     /locus_tag="PK710_12465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOT10581.1"
                     /translation="MTTVRVIRDILEANDRIARDNAGLFSRSGVFVINLMSSPGAGKT
                     TLLERTIERLAQRMKIGVIEGDIQSSIDAERIERHGVKAVQINTGGACHLDGNMIRDA
                     LDEFDLDTLDLLVVENVGNLVCPAEFQVGEDMKVMLLSVTEGDDKPLKYPLMFQQSRA
                     LVIHKIDLLPYVNCSVEAIEQRARALRNDIEIFQVSSTRGDGLEPWVEWLASQVANKR
                     G"
     gene            5217..7511
                     /gene="hypF"
                     /locus_tag="PK710_12470"
     CDS             5217..7511
                     /gene="hypF"
                     /locus_tag="PK710_12470"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOT10582.1"
                     /translation="MVDLRRVRVELQGVVQGVGFRPFVFDLACRWNLSGWVLNTSSGV
                     TLEVQGERLDLEAFLHALVDQPPPLAFIVEVHTSDRNPIPEQQGFEIRKSHAFAQRSA
                     IMPPDTCVCDDCLAELHDPKDRRYHYPFINCTHCGPRYTIIRDIPYDRDKTSMASFTM
                     CEACRREYDDPHDRRFHAQPNACFACGPRIWLTDATGATVPSDDPIRQTSAWLREGKI
                     VAIKGLGGFHLAVDATQPEAVQCLRERKQREYKPLAVMCRDLQEARRHVLVTELASKL
                     LLSRHRPIVLLPKRAEAGLAEQVAPGSADLGIMLPYTPLHVLLLEQGPATLVMTSGNV
                     SEEPIAIGNEEAIERLATIADYFLLHDRDILVRNDDSVLAPSGTQPVFFRRSRGYVPL
                     PVMLREPGESVLGVGAELKNTVCLTRGRMAFMSQHVGDLSNVAAVEAFKDTIDRLQRI
                     VDARAVCVGVDMHPDYPSTGWAESLDIPRVYVQHHHAHVAACLAEHGESGKAIGLALD
                     GTGLGTDGAIWGGEVLLADLESFQRMGHLGYVRMPGGDQAARQPWRMAVSYLIEAFGA
                     EWQRHCPQSLLAIDASRRQAVATLVERGFQAPWTSSTGRLFDAMAALCGLHLEAKFEA
                     QAAMALEMTAGQAMHDDDVWPVSWGSGEVLCLRSSSFIRHGVEDLRKGVPISSVAMRF
                     HRSLAEAFVNVCVVVRERTGCELVAAGGGSMQNRLLSSLLVRGLRSRGFRVLVPERLP
                     PGDGGIALGQAVVARTRWTKLGVN"
     gene            complement(7513..>8343)
                     /locus_tag="PK710_12475"
     CDS             complement(7513..>8343)
                     /locus_tag="PK710_12475"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT10583.1"
                     /translation="SLHWLDTHHVDAVVIATPGPWHTQHALTALQAGLDVFVEKPMAT
                     QATDARKLAAAFHATDRIGMVGHLMHHHGAIHSLFEAIRQGRIGTPLAFHSARLCRPN
                     SRDVDGSLLFSLAPHDISLLRALDASAIRHIDTKWGRMGSLLPNIANLHLRLSSGLEA
                     HIVVSRAHHTKVRKTTIVGEHASIVFDDVTSGPKLTLRHHGKSEALDFDPTPPLRTEI
                     REFIHCIRTRKNPTIGTREGLEVVEILERAQAIAMRHDDRNVTRKSSSPSFPWKDFAL
                     "
BASE COUNT         1781 a         2231 c         2533 g         1798 t
ORIGIN      
        1 aggatcaccg cgagcagtgc atcgccttcg aaccccgcga ggacggccaa aatcggagcc
       61 aaaggaaggt tgaaaacgga agcccagaac agcaattcat gccatccggc ccgatgccaa
      121 tcccgcggaa tataaggagg attttccgat ttgaccgccc gtggcggcag gcccaatcga
      181 gcgtcgttga tgcgccacga gcttagctgc tcgggggccc aggcgttctt tcgccatcgc
      241 ttccgaatcc acgtccacat cgtttccgtg tcctgtcaca gaccttcagg atcaacaaat
      301 aaagtctcgt cgacgtgtgt ccgacaccca cgatgaccgc accatttcca tgtccgaacc
      361 cctacacgat tcgaaatgga atgttttcac agcgttggac gcgttttccc tcggctcccc
      421 catgctactc tgccggggca aaatgatgga aaacctccgc ctcatccgat tgatgatggt
      481 tgcgtgcctc gcgagttgcg gacgagaaac cgaaaaaaaa accgagcctc cccctgtgcc
      541 gaatgcggta gctcctaccg tcgcggtatc ctccgcgaag cctgcccacg tagagccggt
      601 gcgaatcgaa accgtgcccg tggcggggga cctgcctgtt ttcgtccttc gtggggcaac
      661 agccgcgcga cgcatggttt ttttgcatgg gcattgcagc cacgccctgg gttttttgca
      721 atcgttccca catgccgccg ctcgccatgg cacgatcgtg gccgtacaag cggataaggc
      781 ctgcgctgac gaaaactatc gcgaatggac ggatactccg gaacggctcg atcgacggat
      841 cgaagcggcg ttccgaacgc ttggttcctc cgactccctc gatgggctgg ccattcttgg
      901 gtattcgtcg ggagcggtgt tggccgagat gctcgcccac cgatctccca agcgctattc
      961 gaacgtggtg ttgattggag gccctcaaaa acctgtggaa tggcgacttc gacggacccg
     1021 ctcgacggcg atggtggccg gtgaacggga tcgacaggat ttgatgcaga tgggcgcaag
     1081 ggagctgcaa ctatcgggaa ttccttcgaa attttttata ttaccgggtg ccaagcacgg
     1141 ccaaatgggt ccccaggcgg aagccgtgat ggagcaggtt ctcacttggc tttgggataa
     1201 cgaacgcgaa atctagttac ggacgctggt aaatgctcac gatcggaacg tcgtcgtgcg
     1261 tgacgatata aaccggcgca cgcgtcccaa acgccaccca catctggaat tcgacttcat
     1321 tcatgtgaag ttcgtgttga acgagggcat agtctccttg ggacggggct cccacgcctc
     1381 gcagatccga acggatcctt ccttcgtcga tcatccgtgc ccaggaatcc cacgccgtat
     1441 catgaatgaa gaccgtggct ccttgttttg cgtgttctgc caggaattgc tcgctggcgt
     1501 tttggctggt gtatccccaa aattgcctgt ttaatccaag gcttgcgccg cctttggtgc
     1561 ctccaatcca gggcatgtag ctggccaaac caaagggatg cgcatgcatc gtgatcgcca
     1621 ggggaccggc gagcacgaag gccacgactg ctgttttggc aaccgcaacc caccttgtgg
     1681 accatcgcgt caagaccgag gataaagccc gtagggaaag ctccagacca tgtgcggcga
     1741 agagcgccat gaacggatag gccgtcatcc aatgtttggt tccaccaaaa atcggggttt
     1801 tggcaaaaaa ccacggggaa atggccgcga tgatcgccag ggcaaagagc atatcgggat
     1861 acgttgggcg ctctggttcg gtgagagtgt tttgtttcca cgctcgcaac catccaagcg
     1921 cgcctctgac cttttgaccc gcgcccacgg cgcataacaa aagcgtgatg ctgggaaccg
     1981 tagccgcgat catcagcggc atgtacgcgc ggggcgaagg ggcatcgaag tagttcgtcc
     2041 ccagaaactc catgttgtaa tagacatggt tgaagtgaaa atcgaagtac tcccgcaaac
     2101 gagccgcggt gtcatgccac atccacggcc ataaggccca gaacaccagg gggcccacga
     2161 tagccatggc gagcagggag gtaggaacgc gcaggatctg atctttccag tctcgacgca
     2221 gatgccggtg tacgacgagc accgcatgag ggagaaatac cgctggcaag atccaggcat
     2281 tgtgtttggt gtcgagggtc aacccgtaga caacgcccgc caacagcgcc catccaagcc
     2341 ctcctttttc gatagaacgc caataaatat aaatacatag cgtccacagg gccacgatgg
     2401 gaaggtcgaa gcaagccagg tgggcatgat agaaaacgcg tggggtaagc aagaaaaggc
     2461 acgcggcgat caagcccgcc gtacgtgaat acacgcgcgc gccgaatagg taggtaaccc
     2521 atacggccac gcccgaaagc gccatcgccg gtaaacgaaa cgcgtcgctg gcgtgggaaa
     2581 acaccttcca tttctcgtga aacgctagcc aggacagaga aaacaggctt ttgatgagag
     2641 aaggatgctc gtgattgtac ccccaaatcc gatcgatggt cgcccgtgaa aaggcacggg
     2701 atggatcgga gacgagcaag ttgaaccatt cggcatattg tgtggccgca cgaaaataaa
     2761 acccttcatc acgcgcgaat ccaatggcat ccgccgtgga aagcagccaa gccgtcacga
     2821 gaatcgccat cgtggcgccg aggatctgat cgcgccatcc aatgtccgag gggagctgaa
     2881 agagcttttt caccgggtgg ttacctcgaa acaataggtt ctacggtctg cgcgggtaga
     2941 cgatatctca gcttgcaatt ccgccgtttt gcccgcacgg gaggtcgtat cgaaggcaaa
     3001 cgaactccat ccttgtccgt cctcgtggat ggcctcgccc aacgtctcgt cgtccgaaga
     3061 aaaaacaatg cgtaccgctg ggcctttgcg atctcgttcc gcttccacat aaagggcgtg
     3121 atgacctagc aaatgcgttc cgaaacgaac ggatttccat cgcaatcgca gcacttcctg
     3181 gcctccggga ggcggcgcgt ggatgcatcg acgaggcttg tactcgaggt ccgcgttgat
     3241 cgtctcaccc acccaagtgt tgcggcaatg gtaccgatcc gaaggttgcg cgggtccaaa
     3301 cccgagaggt cccgtcacag gttgcccatg tcgccattca cacggttgca cggcctcccc
     3361 ccgtaccaaa gacacatcca tttggtgtgc cgtcgcatgg gaaaccagat cgtcgatcac
     3421 cggatgagga tccgggttgc gcaagcgacg catttgcagc gctccccatg actgctcttg
     3481 ttcgagatgc cacgaagcca gggccgggtg acgagccgat ccgagggaaa cttcgatggc
     3541 tacgggaaaa cgggattcgt ccgcacgcgc cacgcgctgc accgtcatga gcccgtcacc
     3601 gagttgcaag cgccccaaag gttcgagcca cgatggtgac accaccacga gatccgatgg
     3661 ttgaacttgc gcttccaact gcgagcgcac ggccgtccaa tcctccggtt tcaagaccgg
     3721 tcgcgtctgg ctgatatgca aacccaactc aagcggtgcc accaacaaca ccggaacaaa
     3781 ccaccatctt tgaatccagg ccatgaacct ggatttcgag gtggtaccaa ggttggatcg
     3841 agcgtcttcg ttcacgcggt tttccatgcg tggcagcgag gggcattcct agcacaacct
     3901 tgcggacaag cgatcttccc ttggttgggt tctcgcgagc cgacgcatgg ttcccgaaat
     3961 ctggtgtctc ccgcgcctcg tcatcgcgaa aaataaaggc tccgtcttcg ggtggacacc
     4021 taaaatattc atttaaatca atagcttatc tcataaacgg aaggccttgg gagcagcttc
     4081 gccccccgtc cggttgtcgt tgctcaaccg cgccaatccg cgggctaggg acgatatggc
     4141 agcggaattc cggtggattt gggtgatagg tcttggtggg gtgacgagga ttgacgccca
     4201 tgggttcggg ctttagacca cgcgctgtgc atgaactctc gatagcccag tcgattctcg
     4261 atacggttct cgagcaagcg gacaagcacg gagcggttcg tgtccttcga atccatcttc
     4321 ggatcggcca attgacggcc gtcgtgcaag aggctctcga tatggcattt gaagttctca
     4381 cgcgcgatac gattgcggct cacgcgcgaa tcacggtgga acgggttccg tggcgtgttc
     4441 ggtgttcgcg gtgtgaacat gagtatagcg tgcaaaacga tctgccgacc tgttcacgat
     4501 gtgggcattt ggggggtgag acgcttggtg gacgcgagct gcagatcgtg gaaatggaca
     4561 tagaatgact actgttcgtg taattcggga catcctcgag gccaacgatc gcatagcccg
     4621 ggacaatgcg ggtctatttt cccgttccgg cgtattcgtg atcaatctga tgagttcgcc
     4681 aggagcgggg aagaccacct tgctcgagcg aaccatcgag cgtcttgctc aacgcatgaa
     4741 aattggcgtg atcgaaggag acattcaaag ttccatcgac gccgagcgta tcgaacgcca
     4801 cggtgtgaaa gcggtgcaga tcaacacggg aggtgcctgt cacctggatg gaaatatgat
     4861 ccgtgatgcc ctcgatgaat tcgatctcga caccttggac ttgctcgtgg tggaaaacgt
     4921 cggaaatctc gtctgtccgg ctgaatttca ggtaggcgag gacatgaaag tgatgttgtt
     4981 gagtgttacc gagggagacg acaagccgct caaatatccc ttgatgttcc agcaatcccg
     5041 agcgttggtg atccacaaaa tcgatctgtt gccttatgtg aattgctccg tggaggccat
     5101 cgagcagcgg gctcgggcgc ttcgcaacga tatagaaatc ttccaggttt ccagcaccag
     5161 aggcgatggc ttggaaccat gggtcgaatg gctcgcctcg caggtggcaa acaagcgtgg
     5221 ttgatctccg acgcgttcgt gtcgaacttc aaggagtcgt gcaaggcgtg ggcttccgcc
     5281 ctttcgtctt cgatcttgcc tgtcgatgga atctttccgg gtgggtgctc aatacctcga
     5341 gcggagtcac cctcgaggta caaggcgaac ggctggacct cgaagctttc ctccatgcgc
     5401 tcgtcgacca gccccctccc ttggcattca tcgtcgaggt gcatacctcc gatcgaaatc
     5461 ccatcccaga acagcaagga ttcgaaattc gaaaaagtca cgctttcgcc caacgttccg
     5521 ccatcatgcc cccggatact tgtgtttgcg atgattgcct ggccgaactg cacgatccca
     5581 aagatcgtcg ctaccactac ccgttcatca attgcaccca ttgcggccct cgttacacca
     5641 tcatccgcga tatcccttac gatcgagaca agacgtcgat ggcttctttc accatgtgtg
     5701 aagcttgccg tcgtgaatat gacgatccgc acgatcggcg gtttcacgcg cagcccaacg
     5761 cttgctttgc gtgtgggcca cgtatctggc tcacggacgc cacaggagcc acggttcctt
     5821 ccgacgatcc aatccgtcag acgagtgcat ggcttcggga aggaaagatt gtggccatca
     5881 aaggtcttgg ggggtttcat ctcgcggtcg atgcgacgca gcctgaggcg gtgcagtgcc
     5941 ttcgtgaaag aaaacaacga gaatacaagc cgttagctgt tatgtgccgc gatctgcagg
     6001 aggcacgacg gcatgtgttg gtcacggagt tagcttcgaa gctgctgttg tcgcggcatc
     6061 gtccgattgt gttgctgccc aagcgagcgg aggcagggct ggcggagcag gtagccccag
     6121 gcagcgccga cctgggcatc atgcttccct acacgccttt gcacgttctg ttgttggagc
     6181 aggggcctgc cacgttggtg atgaccagtg gcaatgtatc ggaagaaccc atcgcgatcg
     6241 gcaacgagga agcgatcgag cgattggcta ccatcgcgga ctatttcctc ttgcatgacc
     6301 gggacatctt ggttcgcaac gacgactctg tgttggctcc cagcggaacc caaccggtgt
     6361 tttttcgccg ttcgaggggc tatgtgccac tgccggtgat gttgcgtgag ccgggcgaaa
     6421 gcgttcttgg ggtgggcgcg gagttgaaaa acacggtgtg tttgacgcga ggacgcatgg
     6481 cttttatgag ccagcacgtg ggggatttat ccaatgttgc ggccgtggaa gcgtttaaag
     6541 acacgatcga tcgtttgcag cggatcgtcg atgcgcgcgc cgtgtgtgtg ggtgttgaca
     6601 tgcatccgga ctatccttcg acgggatggg cggaatctct tgatattcca agggtttatg
     6661 tgcagcacca tcatgcccac gtagcggcct gtttggcgga gcatggggaa agcggtaagg
     6721 cgattggttt ggccctcgat ggtacggggc ttggtacgga tggggcgatc tgggggggtg
     6781 aagttttgtt ggcggatttg gagtcgtttc aacggatggg acatttggga tacgtgagaa
     6841 tgccgggggg agatcaagcg gctcggcaac cctggagaat ggcggtcagc tatttgatcg
     6901 aggcgttcgg tgcagagtgg caaaggcatt gtccgcagtc gttattggcc atcgatgcgt
     6961 cgcgaaggca ggcggtggcc acgctggtag agcgtgggtt tcaagcgccg tggacatcga
     7021 gtacggggcg gcttttcgat gcgatggcgg ccttgtgtgg gctgcatctg gaggcgaaat
     7081 tcgaggcgca ggcggcgatg gccttggaga tgacggctgg gcaagcgatg cacgacgatg
     7141 atgtgtggcc ggtgtcgtgg gggagtggtg aggttttgtg tctccggtcg tcgagcttca
     7201 ttcgtcacgg tgtagaggat ctacgcaagg gcgtaccgat atcctccgta gccatgcgtt
     7261 ttcatcggtc gttggcagaa gcttttgtga acgtttgcgt ggtcgtcagg gagcgaacgg
     7321 gatgcgaact cgtagctgcg ggaggtggca gtatgcaaaa ccgcttgctg tcttcgctgt
     7381 tggttcgcgg gctacgatcg cggggatttc gtgttcttgt gcccgagcgt ttgcccccgg
     7441 gggatggtgg aattgcgttg ggacaagcgg tggtagcaag aactcgttgg acgaagttgg
     7501 gagtcaatta ggtcaaagag cgaagtcttt ccatgggaag gagggactgc tggatttgcg
     7561 tgtcacgttt cgatcgtcgt gtcgcatggc gatagcttgc gcacgttcca agatttccac
     7621 gacctcgagc ccctcacgcg ttccgatggt cgggttcttt cgcgtacgaa tacaatgaat
     7681 gaattcgcgt atttcggtac gaagcggtgg tgtgggatca aagtccagcg cttcgctttt
     7741 tccgtgatga cgcaacgtga gtttcggacc gctcgtgaca tcgtcgaata cgatcgaggc
     7801 gtgttctccc acgatggttg tttttcgtac tttggtgtgg tgtgcgcggg agactacgat
     7861 atgagcttcg agtccgctcg aaagccgcag atggaggttg gcaatattgg ggaggaggct
     7921 tcccatccgt ccccatttcg tgtcgatgtg acgaatggca gaagcgtcga gggctcgcaa
     7981 caacgagata tcgtgagggg caagggaaaa gagaagggag ccgtcgacat cgcgggagtt
     8041 cgggcggcag agtcttgcgc tatgaaaggc gagaggtgtg ccgattcgac cttgtcgaat
     8101 ggcttcgaac aagctgtgaa tcgcgccgtg gtgatgcatg agatgaccca ccatgccgat
     8161 tcgatccgtg gcatgaaagg cggccgcaag cttgcgtgca tcggtggctt gggtggccat
     8221 gggtttttcg acgaatacgt ccaatcctgc ctgtagggcg gtgagagcgt gttgggtatg
     8281 ccatggcccc ggtgtggcga tgacgaccgc atcgacatgg tgagtgtcga gccaatgcag
     8341 cga
//
LOCUS       DAOSOR010000211         8336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8499_length_8336_cov_4.148291, whole genome shotgun sequence.
ACCESSION   DAOSOR010000211 DAOSOR010000000
VERSION     DAOSOR010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8336
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8499_length_8336_cov_4.148291"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            347..1708
                     /locus_tag="PK710_12480"
     CDS             347..1708
                     /locus_tag="PK710_12480"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HOT10584.1"
                     /translation="MAIITISRGTRSGGTQLAQHLGERLGYKVLSREVIVDAAKTYGV
                     SEDRLADGLEHTPGLWARFTGHEENYLLAIQATLADHVSEGNVIYHGLAGRFLLSGLP
                     GVLRVRLIAPLSYRVSATAAHLGITRDQALMHIQKIDHEREKWVRSLYGEDWADPNHY
                     DIVINLGDMSMDTATSLVQCMASSKEFAWTKEAQRQVKDFALRNRVRAHLRFRSPWPE
                     LPVNVKVKDGIVLLSGNNAFEALRHDVEQFVRGIEGVTLHSPTEKSSPATEHAHGELY
                     ARDIMIPLNRYPHVRDSDSVRDAIGAIASSAVRLEDGFTISPRYVLVLDANQSLVGVL
                     SRRDLIRGLAPGFLNVLHTLENVSSVVPPVADSFDHAFAWNSLLSSGALENAKERVSA
                     VMLPPKAVLHTHDTLGTVVSAMLHHKVDLIPVQDQGLIVGVVLMTDVFDSVAQFIMEG
                     GRS"
     gene            complement(1731..3563)
                     /locus_tag="PK710_12485"
     CDS             complement(1731..3563)
                     /locus_tag="PK710_12485"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOT10585.1"
                     /translation="MSALRIAIASGKGGTGKTTVATSLAVALQMAGKRVVFADCDVEE
                     PNGHLFLRPQITSSRDVTLPVPEVDLAACTFCGRCAEVCRYSAIVVLPNQVLTFPKLC
                     HSCGACTLACPENAIREIPRAIGVVESGQAEGLSFVQGRLNVGEAIAPPVTRAVQEGL
                     PECDVVIVDAPPGTSCPVIESVKSADVVLLVTEPTPFGLNDLELAVQMVRTLGIPFGV
                     AINRADGGDGQVVAYCEREGIPVVLELPNDRAVASAYARGEMAVRAKPVWESKFINLF
                     NELYKLSRQKVSPVPAPLELEKVSASPPEVNPIRLGPSHPVQELVVISGKGGTGKTSV
                     SASLFALAGQAAIADCDVDAADLHLVLNPEIRQQHAFSGGSTAIVLPERCTSCGICAE
                     HCRFDAIGPAFDAPTYVVNPIACEGCGVCELVCTERAIEMPARINGKWFISETAYGPM
                     VHARLGIAQENSGKLVSLVRKEAKAVAQRTKKSLLISDGSPGIGCPVIASISGASLVL
                     VVTEPTLSGLHDLERVVALTRQFRVRAVVGINKADIHEPMARRLEDRAKELGLPVVGK
                     IRYDDAVTRAQVQRIPVVLGPDSAAAQDIRAMWETLQPLLFEAS"
     gene            complement(3560..3907)
                     /locus_tag="PK710_12490"
     CDS             complement(3560..3907)
                     /locus_tag="PK710_12490"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT10586.1"
                     /translation="MSKSFPQGKTNGIPLSKAHPWPWNQASSGLQGTLDASAIEVFSS
                     CPGPNHAPVAVLADEKQCIGCGFCLGACAYMAISFDVLPVIHPGLCQGCGDCVQGCPV
                     GALELVARKDRHA"
     gene            complement(4001..4360)
                     /locus_tag="PK710_12495"
     CDS             complement(4001..4360)
                     /locus_tag="PK710_12495"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOT10587.1"
                     /translation="MIIAVSALAPSLDAEVDPRFGRCGYFVLVNTDDMSFQAIENANA
                     ARGGGAGIQSAQLVAKHGAKTVLTGSCGPNAHETLTAAGIGVAVGCSGTVRSAVQQFQ
                     EGKLSLAKEPNVAGHHA"
     gene            complement(4374..4661)
                     /locus_tag="PK710_12500"
     CDS             complement(4374..4661)
                     /locus_tag="PK710_12500"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOT10588.1"
                     /translation="MPGGDGTGPFGQGPRTGRGAGWCAGGAGAGFGGGRGRRMRGHRG
                     VRGWGYGGPMADPAPVDGRQSLATPFDALLEKLDDLSERLARLESREPKPE"
     gene            complement(4700..5071)
                     /locus_tag="PK710_12505"
     CDS             complement(4700..5071)
                     /locus_tag="PK710_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10589.1"
                     /translation="MLVAVTTHQDRVSPVCDVARCFLVVETDGNREIGRSAVTLDAMA
                     LYDRARRMTSFGWDVLICGAISRPFQMMLTAGGLRVIPNTCGAVQDVVRAFFDGSIDH
                     GSFRMPGCGCCGRGRRRRRGR"
     gene            5230..6636
                     /locus_tag="PK710_12510"
     CDS             5230..6636
                     /locus_tag="PK710_12510"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT10590.1"
                     /translation="MPSRPSKTVRAAPAGQGNCRDPVLDSINEGVFTVDGDWRITTFN
                     RAAEKITGISREEAIGQRCSEVFRATICEGSCALKQAIATQKPVVNTTVTIIDARGRR
                     IPIKVSAAILRGEDGKALGGVETFQDLRQMEELRKQLLDKQKYMDIVGKSAPLQRLLQ
                     LLPAVAESDAPVLIQGPSGTGKELFAKAIHQLSPRKDGPFVAVNCGALPDTLLESELF
                     GYVAGAFTGANKDKPGRFALAHQGTLFLDEIGDISPALQVRLLRVLQEMTFEPLGAVK
                     SIHTNVRIISATHRNLTDLVQQGTFREDLYYRIRVVGLDIPPLVNRREDIPLLMDHFI
                     DTLNRLHGFEVAGISPEAYALLLEHDFPGNIRELRNVLEHAFVLCHQGLIRPEHLPAY
                     LRRDPALPPSPPHRTSLHLPSSEREMIQAALQKHTGNRALAARELGIDPSTLYRKIKR
                     LKLPLPERDGRGKRSWLP"
     gene            6799..>8336
                     /locus_tag="PK710_12515"
     CDS             6799..>8336
                     /locus_tag="PK710_12515"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOT10591.1"
                     /translation="MARADTKQAEVLHEYAAIRVLLIDEEERFRVYLRSLLDTRGFDI
                     READSPEEGMRILRQEHQDVVIFDRKMSTMSGVDMLREIKRVAADTQVVMLTGHASKN
                     SAIDKIPGVFAFLEKPCETEGLVATLRAAARHRQQSAALLSSRPKPSSVWAWLKGTHN
                     FRPGVMILGAILFTMTCLAPTPPTLLSFLNVSRLEQRQSDPIASYARYSRMKPGETIA
                     AQYSTFARQQKKVVAPDGTVIFRPLDAQEVGFRARVMLGILVLAGLFWATGALPFTMT
                     AMLVGFLMYVFRVFSPDMVAKAFAKDSVLFILGILALSVGIMNSKLDRRLGLLLLRTS
                     TSLPRYLFFFLPLLAVCASFLSEYALVAIITPTLMLAYRAALDSSGIAKDRSLAVLLV
                     LSMCFAANQGGPGSPAAGGHNAIMMGILADYGKAPSFSNWLTYGLPFVPVMALVIASY
                     FFFVVYRKVSTQSLNIAAILQAESKNQGPLSRRELITIATLVLVLLLWLLGDAVDTGF
                     GLGGP"
BASE COUNT         1802 a         2655 c         2157 g         1722 t
ORIGIN      
        1 caggtcgaaa agatactcgc cctgggactt ctcctccatg tccccaataa tcttgagaac
       61 ttgattgttc tgctcgagca gcacaagaaa acgtttgaat tgctcttgaa cgtctcttag
      121 ctgcgcttcg gcttcctgtc gctgcggcgg gagtgtcacg gcagggtctc tcttattttc
      181 cttcgaacca cgactccccc tgctctcgca tggaaatggg gccaaagcaa cgtctcccac
      241 cctccacacc ccccaacgag cccctctccc cctgtcgcaa ccctcgacgc tctacccttg
      301 tccaactatg ttcggtacga ttcaatcgat tcaatagggt ggattcatgg cgatcatcac
      361 gatctcgaga ggaacacgca gcggcggcac ccaactcgcc caacacctgg gcgaacgact
      421 cggttacaaa gtgctgagcc gagaggtcat tgtcgatgcg gccaaaacct atggcgtatc
      481 cgaagacaga ctcgccgacg gactcgaaca tacccccggt ctttgggctc ggttcacagg
      541 acacgaagaa aattacctgc tcgcgatcca agccaccctc gcagaccacg tttccgaagg
      601 aaatgtcatc taccacggac tggctggccg cttcttactc agcggcttgc caggcgtctt
      661 gcgcgtccgc ctcatcgccc ccttgtccta ccgcgtaagc gccaccgccg ctcacctcgg
      721 catcacccgc gaccaggccc tcatgcacat tcagaaaatc gatcatgaac gcgaaaaatg
      781 ggtgcgaagc ctgtacggtg aggattgggc cgaccccaac cattacgata tcgtcatcaa
      841 cctgggcgat atgagcatgg ataccgccac cagcctcgtg caatgcatgg cctccagcaa
      901 agaattcgcc tggaccaaag aagcccaacg ccaggtcaaa gacttcgccc tgcgcaatcg
      961 cgtccgagcc catcttcgct tccgctcccc ctggcccgaa ctgcccgtca acgtcaaagt
     1021 caaagacggc atcgtcctac tctccggcaa caacgccttc gaagccctac gccacgacgt
     1081 cgaacaattc gtgcggggaa tcgagggagt cacactccat tcccctacag aaaaaagctc
     1141 tcccgcaaca gagcatgccc acggcgaact gtacgcccgc gatatcatga tcccgttgaa
     1201 tcgctacccc cacgttcgcg attccgactc cgttcgcgat gccatcggtg ccatcgccag
     1261 ttcagccgtc cgtctcgaag atggcttcac catttcaccc cgctacgtcc tcgtgctcga
     1321 tgccaaccaa tcgctggtcg gcgtcctgag ccgacgtgac ctaatccgtg gtctcgcccc
     1381 cggcttcctc aatgtgctgc atactctcga aaacgtatcc agcgtcgtac cccccgttgc
     1441 ggattccttc gatcacgcct tcgcctggaa ttcccttctg agcagcggcg ccttggaaaa
     1501 cgccaaagaa cgcgtctccg ccgtcatgct cccccccaaa gccgtccttc atacccacga
     1561 caccctcggc accgtcgtca gcgccatgct ccaccacaaa gtcgacctca ttcccgtgca
     1621 agatcaagga cttatcgttg gcgtggtact catgaccgac gttttcgatt ccgtggctca
     1681 gttcatcatg gaaggcggcc ggtcgtaaga actaactatc cgaaaatgga ttatgaagcc
     1741 tcgaaaagca gcggttgcag cgtctcccac atcgcccgaa tgtcctgcgc cgcggcggaa
     1801 tccggcccca atacgacggg aattcgctgc acttgcgccc gcgtcaccgc atcgtcgtaa
     1861 cgtatttttc caaccaccgg caagcccagc tccttcgctc gatcctccaa tctcctggcc
     1921 atcggctcgt gaatatccgc cttgttgata cccacgaccg cccggacacg aaactgcctg
     1981 gtcagcgcca cgacccgctc gagatcatgc agccccgata aggtaggctc cgtcaccaca
     2041 agcaccaaac tcgcaccact gatcgacgca atgacgggac aacctatccc cggtgaacca
     2101 tcgctgatga gcaacgattt tttcgtccgt tgcgccacgg ctttggcttc cttacggacc
     2161 aaagaaacca gcttgccact gttttcttga gcaattccaa gacgagcatg aaccatgggc
     2221 ccgtacgccg tctcggagat gaaccacttg ccattgatcc gcgcgggcat ctcgatggcc
     2281 cgttccgtac agacaagctc gcaaacccca caaccctcgc aagcgatggg attgaccacg
     2341 tacgtcggag catcgaacgc agggccgatg gcatcgaaac gacagtgttc cgcgcatatt
     2401 ccacaagaag tgcagcgctc gggcaacacg atcgccgtgc ttcctccgga aaatgcatgc
     2461 tgctggcgaa tctccgggtt caacacgaga tgcaaatccg cggcatcgac atcacaatcc
     2521 gcaatggccg cctgtccggc aagcgcaaat agggaagccg aaacgctcgt ttttcccgtg
     2581 cctcccttgc cactgatgac caccaattct tgaacagggt gggaaggacc cagtcgaatg
     2641 ggattgacct ccggcggcga cgccgagact ttttccagct cgaggggtgc cggtaccggg
     2701 cttaccttct ggcgggataa cttatataac tcattgaaaa gattgataaa tttgctttcc
     2761 cagaccggtt tggcgcgcac ggccatctcc ccccgcgcat acgcgcttgc cacggctcga
     2821 tcgttgggca actccaacac caccgggatg ccctctcgct cgcaatacgc aacgacttgc
     2881 ccgtcccccc catccgcacg attgatcgct acaccaaaag gaatcccgag cgttcgcacc
     2941 atctgcacgg caagttcgag atcgtttaaa ccaaagggcg tgggctcggt gaccagcaac
     3001 accacatcgg cggacttcac cgattcaatc acgggacagg aggtgccggg gggcgcgtcg
     3061 acgataacca catcgcattc gggcagtcct tcctgtacgg cccgcgtcac cggcggtgcg
     3121 atggcttctc ccacgttcaa acgcccttgc acaaaagaga gcccttcggc ctgaccgctt
     3181 tcgacgacgc cgatggcgcg tgggatttct cgaatggcgt tttcggggca ggccaacgta
     3241 caagctccac aactgtgaca gagtttggga aaggtcaaaa cctggttggg taggaccacg
     3301 atggcggaat aacgacagac ttccgcgcaa cggccgcaaa acgtgcaggc cgcgaggtca
     3361 acctcgggca cgggaagggt gacatcgcgg gatgacgtga tttggggacg aaggaagaga
     3421 tgaccgttgg gctcttcgac atcgcaatca gcgaaaacta ctcgttttcc cgccatttgg
     3481 agagccaccg ccaggctggt ggcgaccgtg gtttttccgg taccgccttt tccactggca
     3541 atggcaattc gcagagcgct catgcgtgcc tatcctttcg cgcaacgagt tcgagagcgc
     3601 cgacgggaca tccttgcaca caatcgccgc agccttggca caggcctgga tggatgacgg
     3661 gaagaacgtc gaagctgata gccatatagg cgcaggcccc caaacagaaa ccgcagccaa
     3721 tgcattgctt ctcatcggcg aggacggcta ccggggcatg gttggggccc ggacaggatg
     3781 aaaacacctc gatagcagaa gcgtcgaggg tgccctgcaa gccggaggaa gcttgattcc
     3841 aaggccatgg atgagccttg ctcagaggta tgccgttggt ttttccttgg ggaaaagact
     3901 tactcatatc gacctaacct tcataccgat ccgtcgcgag cgaacgactc gcgtttcggc
     3961 tcgcgaccga cggctgcatc gccaaccatg attcccagcg tcaggcgtgg tgaccggcca
     4021 cgttcggctc tttggcgagc gatagttttc cttcttggaa ttgttgtacc gcggaacgca
     4081 cggttccgga gcaccccacg gccacaccga taccggcagc cgtcagggtt tcatgggcat
     4141 tggggccgca ggaaccggtc aacacggttt tggcgccgtg tttggccacg agttgggcgg
     4201 attggatacc cgcgccgccg cctcgcgccg cattggcgtt ttcaatggct tgaaacgaca
     4261 tatcatcggt gttcaccaac acgaagtagc cacaccggcc gaaacgcgga tccacctcgg
     4321 catcgagcga gggggcaagg gcagaaacag cgataatcat tgtatttcct ttattattcc
     4381 ggctttggct cacgggactc cagccgagcg agccgttcgg atagatcgtc gagcttttcc
     4441 aaaagggcgt cgaagggcgt tgccagcgat tgcctcccat cgacaggtgc gggatcagcc
     4501 atggggcccc cataacccca gcccctcact cctctatggc ctcgcatgcg acgccccctg
     4561 ccacctccga atcctgcacc agcgccaccg gcgcaccatc ccgcgccacg acccgttctc
     4621 ggaccttgtc caaaaggacc agtaccatct ccacctggca tgtcttactc cttcctacat
     4681 ctcgcttatg cgagaacaat catcggccac ggcgacgacg tcgcccgcga ccacaacagc
     4741 cacatccggg catccgaaag gaaccgtgat cgatagaacc atcgaaaaag gccctcacga
     4801 catcttgtac tgccccgcat gtgttgggaa tgacccgcag ccctccggcc gtgagcatca
     4861 tctgaaacgg ccgcgagatg gcaccacaga tgagcacatc ccaaccaaac gaggtcatgc
     4921 gtcgcgcccg atcgtacaag gccatcgcat cgagggtcac ggcgctcctt ccgatttccc
     4981 ggttgccgtc ggtttccacc acaagaaagc atcgggccac atcacagacg ggcgagacac
     5041 ggtcctggtg ggtggttaca gcgacgagca cgcaaaacgg gagagcaatc ttcgggccaa
     5101 tagctaatat cgctaaatct tccttagaac gcgccgattt cgaaaagcgt gggggtgaat
     5161 aatcgtgcat attgcatcaa acaagttgtt ccaattgcaa tttgcatgat tgtggctaac
     5221 gttgaaatca tgcccagccg cccatcgaaa accgtccgcg ccgccccagc gggacaaggg
     5281 aattgtcgag atcctgttct ggactcgatc aacgaaggcg tattcacggt cgatggggat
     5341 tggcgcatca ccactttcaa ccgggctgcg gagaaaatca ccggaatttc tcgggaggag
     5401 gcgattgggc agcgatgcag cgaagtgttc cgcgccacga tatgtgaagg ctcttgtgcc
     5461 ttgaaacagg ccatcgccac ccaaaagccc gtggtcaata cgacagtaac catcatcgac
     5521 gcgcgggggc gtcgcattcc catcaaggtt tccgcggcca ttttgcgggg agaggatggc
     5581 aaggccctgg gaggtgtcga aacgtttcag gacctccggc agatggaaga actgcgcaaa
     5641 caactgctcg acaagcaaaa gtacatggat atcgtaggga aaagcgcccc cctccaacga
     5701 ttgttgcagt tgcttcccgc cgtggccgaa agcgatgccc ccgtgctcat ccaaggtccg
     5761 agtggaacgg gaaaagagct atttgccaag gccattcacc aactatcccc gcgaaaggat
     5821 gggcctttcg tggcggtcaa ctgcggcgcg ctcccggata ccctgctcga atcggagtta
     5881 tttggctatg tggcgggggc tttcacggga gcgaacaaag acaaacccgg gcgcttcgcc
     5941 cttgcccacc agggcactct atttctcgat gaaatcggcg atatctcccc cgccctgcaa
     6001 gtgcgtctgc tgcgtgtcct acaagaaatg accttcgaac cgctgggagc ggtgaaatca
     6061 attcacacca atgtgcgcat catcagcgca actcaccgaa atctaacgga tcttgtacaa
     6121 caaggaacct tccgggaaga cctgtattac cgaatccgtg tcgtcggctt ggatatcccc
     6181 ccgctcgtga atcgtcgtga ggatatccct ttgctcatgg atcacttcat cgacaccctg
     6241 aatcgactgc atggcttcga ggtcgccggt atctcccccg aagcctatgc cctgctgctg
     6301 gaacatgatt ttcccggcaa tatccgcgaa cttcgcaacg tactggaaca tgctttcgtc
     6361 ctatgtcacc aaggactcat ccggccggaa caccttcccg cctaccttcg tcgcgaccct
     6421 gccctccccc cttccccacc ccatcgaaca tccctgcacc tgccaagctc cgagcgggaa
     6481 atgatccagg cggctctgca aaaacataca ggaaatcgag cgcttgcagc tagagaactg
     6541 ggcattgacc ccagtaccct ctatcgaaaa atcaaaaggc tgaaactccc gttgcccgaa
     6601 cgcgacgggc gcggcaagcg ctcttggctc ccttgacccg ctttttgcct gtcgtggcaa
     6661 catagcaaaa aacgcgatac catattgata gctatttcga ttcccaccta tcatcaacca
     6721 aggcaccgtt tcactccatg aagcggattc cggatttccc accatttttc gcagctaccc
     6781 cataggagcc gctcgctcgt ggcccgtgct gataccaaac aagcggaggt tcttcacgaa
     6841 tacgccgcga ttcgggtgct cttgatcgac gaggaagagc gctttcgcgt ttatttgcgc
     6901 tctcttctcg acacccgggg attcgatatc cgcgaggcag acagcccgga agagggaatg
     6961 cggatccttc ggcaagagca tcaggatgtg gtcattttcg accgaaaaat gtccacgatg
     7021 tcgggagtgg acatgctgcg ggagatcaag cgtgtggccg ccgacacgca agtcgtcatg
     7081 ctcacggggc atgcgagcaa aaattccgcg atcgacaaga ttcctggagt gtttgcgttc
     7141 ctggaaaaac cctgtgaaac cgaggggtta gtagcaacac tgcgcgcggc ggcccggcat
     7201 cggcagcagt ctgcggctct cctttcctcg cgccccaagc cctcttcggt gtgggcctgg
     7261 ttgaagggaa cccataattt ccgaccaggc gtgatgattc tcggagcgat cttgttcacc
     7321 atgacgtgtc tcgctcccac gccgcccacg ctcctgtcgt ttttgaacgt gtcacgcctg
     7381 gagcagcggc aatccgaccc catcgcctcg tacgctcgtt attcgcgaat gaagcctggc
     7441 gaaacgatcg ctgctcagta tagcacgttc gccaggcagc aaaaaaaggt cgtggctccg
     7501 gacggcactg ttattttcag gcctctcgac gcacaagaag tggggtttcg cgcgagggtc
     7561 atgctcggaa tcctggttct cgcgggtctt ttttgggcaa ccggcgcgct gcccttcacc
     7621 atgaccgcga tgctcgttgg cttcctcatg tatgtatttc gtgttttttc tcccgatatg
     7681 gtggccaagg cattcgctaa agactccgtg ttgttcatcc tcggaattct cgccttatcc
     7741 gtgggaatca tgaattccaa gctcgaccgt cgcctgggct tgctgctgtt acgcacgagt
     7801 acatccttgc cccggtatct cttctttttc cttcctttgc tggccgtatg tgcctctttt
     7861 ctatccgaat acgctctggt agccatcatc acgcccaccc tgatgctggc ttatcgggcg
     7921 gccctggatt cctcgggcat tgccaaagac cgttcccttg cagtgctgct ggtcctatcg
     7981 atgtgttttg ccgcgaatca aggaggaccg ggctcccccg ccgccggcgg acataatgcg
     8041 atcatgatgg gcatcctcgc ggactatggc aaagctccgt cgttttccaa ctggctcacc
     8101 tacggcctgc cattcgtccc cgtcatggcc ctcgtcatcg cctcgtactt tttcttcgtc
     8161 gtttatcgaa aggtctccac ccaatccctc aatattgccg ccatcttgca ggccgaatcc
     8221 aaaaaccaag gccccctgag ccgtcgcgaa ctcatcacga tagccacgct cgtgctcgtg
     8281 ttgttgcttt ggctccttgg agacgcggtg gatacggggt ttggtttggg agggcc
//
LOCUS       DAOSOR010000212         8328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8511_length_8328_cov_3.959628, whole genome shotgun sequence.
ACCESSION   DAOSOR010000212 DAOSOR010000000
VERSION     DAOSOR010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8328
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8511_length_8328_cov_3.959628"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="PK710_12520"
     CDS             complement(<1..1109)
                     /locus_tag="PK710_12520"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOT10592.1"
                     /translation="MADMDPVKILIDVGIIALLIVGISQFRTPRGARRGNATAALALL
                     LAIVLVLYRNDIVDVGILAVSLIIGGLVGTFIAMKVTMIQIPAMVAFQHGAGGIAACA
                     IAYVELTRATPPTPVGEVSGYLGLTIGAATFSGSMLASAKLAGKLRQTPTVLSGHNAI
                     LLAMLAAIIGLCVYASTGVGVPEAATTLSAALVGALVLSVALGYTFAVRIGGADMPVL
                     ISFLNATAGLAAAFCGVVIQNQLLIACGATVAASGSILTAIMCKAMNRSFIKVLSGGK
                     MAVAALPAKKTEPKPKKEEAPEAAEPASADTDDKPSEEIEKKEEQAKPETKAEPKTRT
                     PEEAMAEAKTALREAESVIIVPGYGMALARAQFQVA"
     gene            complement(1173..1484)
                     /locus_tag="PK710_12525"
     CDS             complement(1173..1484)
                     /locus_tag="PK710_12525"
                     /inference="COORDINATES: protein motif:HMM:NF024179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOT10593.1"
                     /translation="MTELALMLVVFILSFVIGYVLISRVPPLLHTPLMSMTNAISAVT
                     ILGALLLFAQPSMDLDEKVLGAIALITAAFNLVGGFVITDRMTGLFKSHHEPSGGGGK
                     K"
     gene            complement(1517..2677)
                     /locus_tag="PK710_12530"
     CDS             complement(1517..2677)
                     /locus_tag="PK710_12530"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOT10594.1"
                     /translation="MHIVIPKEILNAEKRVAAIPETVKKYVAKGFTVTVESGAGAGVF
                     ITDEEYAAAGAKIEKDVEKLFASAEVILKVKQPIFNTAVNKHEVEMMPKGSTLVTFLH
                     PAAPDSHDMVRMLRDRGVTAFTMDGIPRISRAQRMDALTSMSTVTGYRAVLDGAELLP
                     RFLPMIGTAIGPIKPANALVVGAGVVGLQAIATAKRLGAVVKAWDIREAARTEAGSLG
                     AKVVGFDVPKELAIAEGGYAKLLPPDWLKKEQEAIMDVVAESDLIVLSALVPGTLAPT
                     IVTKEAVERMKPGSVIIDVSIDQGGNCALTKPGQEILHNNVRVCGVQNIPGRMAMHAT
                     WLYANNMFYYIDNLYKKVPGGPDLEDEIPRTSLVTMQGKIVYQPALDAMGGR"
     gene            complement(2710..3483)
                     /locus_tag="PK710_12535"
     CDS             complement(2710..3483)
                     /locus_tag="PK710_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10595.1"
                     /translation="MNMNPLEVKAKQGQGNYTEIIIKIGSTVVGTRFDELIPCHFPIQ
                     TSQPVYVEIPPSGSKGSTSSFFAETAASSFPENDYPHSIRFPLYQRQENISLCHWEAF
                     VSDASMALSQVPVIAIAAGALCLEADAVVRKYSVLPVWNDTSSVLVRLAVMQNQLSEA
                     SLNPPWQMTVSEGMPLCMGMEQFENNLAAADGNPQSPFELHFTPWLLGARLLGSEGKP
                     IAHRVWQRAWTFDSAEEFFAALRRITGSLRGTVLPKDAA"
     gene            complement(3889..5616)
                     /locus_tag="PK710_12540"
     CDS             complement(3889..5616)
                     /locus_tag="PK710_12540"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT10596.1"
                     /translation="MTTLLQTRAEFGRFRPIAEIGRGGMAEVHLAHTQGPVGFHKLVV
                     LKLVREHLAEDPDAKTMLMDEARLAGRLNHPNVVQTLEVTEIGASPVIVMEYLEGQSF
                     SAILRRAQAEGKPLDLAACLHIVCEALQGIHYAHQLKDFDGSALNLVHRDVSPHNIFV
                     TYDGRVKVLDFGIAKAAITQSETRAGVLKGKVAYMAPEQALGMKVDHRADIYAMGAVL
                     WQIVTGRALWKELPEGQALYKLVQGNVPRPSTINPDVDPRLESCCMKALSTAPEDRYP
                     NALALAEDLESILDSLGRPSTRSLGVVVEGLFDDVRKETQALIEKQLKQHAHGSEDQS
                     SPKLMASGGWHRTAFRSESAVETGDESASRARVSSAAPIIVSSGVLPPKRNRWIVAAA
                     SAGALVVAFGLWTALKPQQPPVAQTAPSAVPSSQVMVAIAASPSAEPVQTSVEITVSP
                     DSARVFVDDVEVKERPARVSFERADSLHRIRAEAEGYKTQQLEVQADSPKSIQLQLAE
                     MPRTVVASSDVKTRPARTTKSDTARETAADIVVPPPPAAVPPAPPPDRQPADSARDRI
                     KNLDRTTPW"
     gene            5941..7176
                     /locus_tag="PK710_12545"
     CDS             5941..7176
                     /locus_tag="PK710_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10597.1"
                     /translation="MDVVTNAKVAFVLERYALDDVSEICLRTRAMATALAERGHDVTV
                     LTTCSRYPGQWNNDVAEGESTIDSLSVVRFRLEQRLVHRLDRPAKWLASYNTLAAKVW
                     GHSVGPVCPGYRPYLDQFGPLFDAVFFTGAWGYLVSEGMGRVRNAVLCPAPWEDRTHQ
                     GKQAATLLRQARGVALASSAEEKRLRTATEYPWSCPTFVIGAAPDPLPSPSYRSKRLT
                     LVDGPFLLAFGHYSPAADRLLEAFQLFRQAHGDTSFEDDRSERMSAKNVRLVLAGDDR
                     FPHDPGNGVVSLGPVDDTARESLMRHALAVVHADPCIRLSTGLLSAWMQGCPTLIHAG
                     STDLQPVLDAIGKECVFDSALTFAAGAASLLSSPGPRRAYGARAQHMVQRVFSPRKLG
                     ECLEQCVRAVMGRAKGQPV"
     gene            7246..8211
                     /gene="galE"
                     /locus_tag="PK710_12550"
     CDS             7246..8211
                     /gene="galE"
                     /locus_tag="PK710_12550"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOT10598.1"
                     /translation="MTVLVAGGAGYIGSHFVRCLEEHGESAVVLDDLSTGFAEAIPDH
                     VPLVRCAIGDRAAVRSVLEEVAPEAVVDFAGLIQVGESMRSPDRYYGVNVSQTIAFLD
                     EIVRSGVKTFVFSSSAAVYGNPIRVPIDEDHPKEPINPYGATKLIVEMAARDYARAYG
                     LRVACLRYFNAAGAHADGTLAERHDPETHLIPLAIDAGLGRRPELTVFGDDWSTPDGT
                     CVRDYIHVMDLAEAHWLAIEKLRNDTPTLSLNLGTGNGYSVRQIIDAVSDVLGKAVPY
                     HIGPRRDGDPPSLVASSNRARNVLGWNPTRSQLDTIVQDAVRSRT"
BASE COUNT         1757 a         2302 c         2427 g         1842 t
ORIGIN      
        1 gcgacttgga attgcgcgcg cgccagggcc atgccgtagc cgggaacgat gatgacgctt
       61 tcggcttccc gcagggcggt tttggcctct gccatcgctt cttccggtgt gcgggttttg
      121 ggctcagcct tggtctccgg cttcgcctgc tcttctttct tctcgatttc ctcagagggc
      181 ttgtcatcgg tgtcagccga tgctggttca gcagcctcgg gcgcttcttc tttcttgggt
      241 ttgggttccg tctttttggc gggcaatgca gcgacagcca tttttccgcc ggaaaggact
      301 ttgatgaagc tgcggttcat cgccttgcac atgatggcgg tgaggatcga accggaagca
      361 gcgacggtgg caccgcaagc gatgagcagt tggttttgga tgacgacacc gcagaaggca
      421 gccgcaagac cggcagtagc gttcaaaaac gagatgagaa ccggcatgtc cgcgccgccg
      481 attcggacgg caaaggtgta accaagcgcg acggagagga ccagggctcc gacaagggca
      541 gcggaaagtg tcgtcgccgc ttcgggcacg ccaacacccg tggaggcgta gacgcacaga
      601 ccaatgatgg cggcgagcat ggcaagcagg atcgcgttat ggccggacaa tacggtcggg
      661 gtttgcctca gttttccagc caatttggcg ctggcgagca tactgccgct aaaggttgcc
      721 gctccgatgg tcagcccgag atagccggag acttcgccaa cgggggtggg gggcgtagca
      781 cgggtcaact ccacgtaagc gatggcacaa gcagcgatac ctcccgcgcc gtgctggaaa
      841 gctaccatgg cagggatttg gatcatcgtg actttcatgg caatgaaagt accgacgagc
      901 ccaccgatga tcagggagac ggcgaggatg ccgacgtcca cgatatcgtt gcggtacaac
      961 acgaggacaa tcgccagaag tagcgccaac gctgccgtgg cgttaccacg tcgcgcgcca
     1021 cgaggcgttc ggaactgtga gataccgacg atgagcaatg cgatgattcc gacatcgatt
     1081 aggatcttga ctgggtccat gtcagccatg atgaactcct cgaaacacgc caaggtcgtt
     1141 gctggtgaag caaagggcaa taggttgctt ggtcattttt tgccccctcc tccactgggc
     1201 tcatggtgcg atttgaagag ccctgtcatg cggtctgtaa tcacgaagcc accgaccaag
     1261 ttgaaggcgg ccgtgatgag cgcaatggcg ccaagtactt tttcgtcgag atccatcgac
     1321 ggttgcgcaa acagcaagag cgcgccgagg atggtcacgg ccgagatggc gttggtcatc
     1381 gacatcaacg gcgtgtgcag aagcggtggc acgcgcgata tgagcacgta gccgatgacg
     1441 aacgacagaa tgaatacaac taacatcaat gccaactcgg tcatgacaca tcctccgggt
     1501 tgccaaggtt atgggatcaa cgcccgccca tcgcgtcgag cgcgggttgg tacacaatct
     1561 tgccttgcat cgttacgagt gaagtgcgcg ggatctcgtc ctcgagatcg gggccgcccg
     1621 gcaccttctt atacaggttg tcgatgtagt agaacatgtt gttcgcgtac agccaggttg
     1681 catgcatcgc catccggccg gggatgttct gcacaccgca aacgcgcacg ttgttgtgca
     1741 gtatttcctg acccggtttg gtcagcgcgc agttgccgcc ttggtcaatg gaaacatcga
     1801 tgatcacgga tccgggcttc atgcgctcga ccgcttcctt ggtcacgatg gtgggagcga
     1861 gcgttccagg aacgagagcg gacagcacga tgagatcgga ctccgcaacg acgtccatga
     1921 tcgcttcctg ttcttttttc aaccagtccg gaggaagcag cttcgcataa ccaccttcag
     1981 caatggcaag ctccttgggc acatcgaatc caacaacctt ggcgccaagg cttcccgcct
     2041 cggtgcgagc cgcctcacgg atatcccacg ccttgaccac ggcaccaaga cgcttggccg
     2101 ttgcaatggc ttgaagaccg acgacaccag caccaacgac gagagcattg gcgggcttga
     2161 tcgggccaat ggcggtgccg atcatcggca aaaagcgcgg gagaagttcc gcgccatcga
     2221 gtacggcgcg atagccggtg acggtgctca tggaggtgag cgcatccatt cgctgcgcac
     2281 gggagatacg cggaatgccg tccatagtga aggccgtgac accacgatcg cgaagcatgc
     2341 gaaccatgtc gtggctatcg ggagcggcgg gatgaaggaa ggtgaccaac gtcgacccct
     2401 tcggcatcat ctcgacctcg tgcttgttga cagcagtgtt gaagatcggt tgcttgacct
     2461 tgaggatgac ctcagcgctg gcgaaaagct tctcgacatc cttctcaatc ttcgcaccgg
     2521 cagcggcgta ctcttcgtcg gtgatgaaca caccggcgcc tgcgccggat tcgacggtga
     2581 cggtgaagcc tttggcgacg tacttcttta cggtttcagg aatggcggct acgcgtttct
     2641 cagcattcag gatttccttc gggatgacga tatgcatgaa actggctcct tgttgatgaa
     2701 cggtccgaat taggctgcat ccttggggag aacggtcccc cgtaagctgc cggtgattcg
     2761 acgaagggca gcaaaaaatt cctcggcact atcgaatgtc cacgcgcgct gccagacgcg
     2821 atgggcgatt ggtttgccct cgctgcctag cagacgagcg ccaagcaacc aaggtgtaaa
     2881 gtgaagttcg aaggggcttt gtgggttacc gtcagcagcg gcgagattgt tctcgaattg
     2941 ctccatgccc atgcaaaggg gcatgccctc cgatacggtc atctgccatg gggggttgag
     3001 ggaagcttcc gatagttggt tttgcatcac ggcgagacgg accaggacgc tcgatgtgtc
     3061 gttccatacc ggcagaacgg aatacttccg taccactgca tctgcttcca agcagagcgc
     3121 gccggccgcg atggcgatga cgggaacttg gctcaacgcc atacttgcat ccgaaacgaa
     3181 agcctcccaa tggcagagcg aaatattctc ttgccgttgg taaaggggaa aacgaatcga
     3241 atgggggtag tcgttttctg gaaacgaaga tgcggcggtt tccgcgaaaa agcttgaagt
     3301 cgaacctttc gacccgcttg gggggatttc tacgtatacg ggttggcttg tctgtatcgg
     3361 aaaatggcaa ggaatgagtt catcgaaccg cgtcccaacg accgtgcttc caattttaat
     3421 tataatttcc gtatagttac cttgcccctg cttcgctttc acttccagtg ggttcatgtt
     3481 catggcagcc aaccctcgac ggaatcccac gacggaacca gcccgctcga tacggaatcc
     3541 gttcggttca tgctccgctg tgtttggcgc agatgggcaa tggatgcgca accgtcagcg
     3601 tgagtaggtt cgacgctagg ccgcggtttc cgtgggcacc atcgggggca accggataat
     3661 gatgggagat gaacaggagt cggcgcttcg caaaagcgga tatgtcttcg acaaggtggg
     3721 ctgaagtaac acctttgttc taaggttatc tcgtcttatt ggatgagatt gatcagaaaa
     3781 ttccctagct cgttttcgaa gagagtacag catttttcac gataggactc ttcgaacaac
     3841 ccgatggctc gccaggcagt agctctgtca gcaggcaaat ccgcgatatt accacggggt
     3901 cgttcgatcg aggttcttga tccggtcacg cgcggaatcg gcaggttgac gatccggggg
     3961 cggcgcaggg gggacagccg cgggaggcgg tgggacgacg atatcagcag cagtttcccg
     4021 agcagtatcc gatttggtcg ttcgagcggg tctcgttttg acgtccgagc tggcaaccac
     4081 cgtacgaggc atttccgcaa gttgcagttg aatcgacttt ggcgaatcgg cttgcacctc
     4141 caattgctga gttttgtaac cttcggcctc cgctcgaatc cggtgcagac tgtccgcgcg
     4201 ctcgaaagac acacgcgctg gtcgctcctt gacttccaca tcgtcgacga agaccctcgc
     4261 gctatctggc gaaacggtaa tctccacgga ggtttggaca ggctccgcag aaggggatgc
     4321 cgctattgcc accatcactt gcgaggaagg cacagcggat ggggcagttt gagcaacagg
     4381 gggctgctga ggcttgagtg ctgtccacag cccgaaagcc acgacaagag ccccggcgct
     4441 tgcggcggca acgatccaac gattgcgttt tggcggaagc acaccggaac ttacaataat
     4501 gggtgcagcg ctagaaaccc gtgcccgaga ggcggactca tcgcccgttt ccacggcgct
     4561 ttcacttcgg aaggccgttc tatgccaacc gccgctcgcc atgagcttcg gggatgattg
     4621 atcctcggaa ccatgggcgt gctgcttgag ttgcttttcg atgagcgcct gggtttcttt
     4681 tcgaacatcg tcgaatagtc cctcaaccac cacgccgagc gatcgcgtgg agggacgtcc
     4741 taacgaatcc aagatgcttt ccagatcttc agcgagagcc aaagcgttgg ggtaacgatc
     4801 ctccggcgcc gtagacaagg ctttcataca acacgattcg agacgagggt cgacatcggg
     4861 attgatggtg ctgggacgag gcacattgcc ttgaacgagt ttataaaggg cctggccttc
     4921 gggcaactct ttccaaagcg cgcgtcccgt gacgatttgc cataatacag ctcccatcgc
     4981 ataaatgtcg gctctatggt cgaccttcat gcccagcgct tgttctgggg ccatataagc
     5041 cactttacct ttgagcacac ccgccctggt ttctgactgc gtaatggccg ctttggcgat
     5101 gccaaaatcg agcactttga cccgcccgtc atacgtcacg aaaatattgt gaggcgaaac
     5161 gtcgcgatga acgaggttaa gggcagaacc atcgaagtcc ttgagttggt gggcgtaatg
     5221 gatcccttgg agagcttcgc aaacgatgtg gaggcaggcg gccagatcca gcggcttgcc
     5281 ctccgcttgc gcacgtcgaa ggatagccga aaatgactgt ccttctaaat attccatgac
     5341 aatgaccgga cttgctccaa tttcagtaac ttcaagagtt tgcacaacat tgggatgatt
     5401 gagacgccct gccagacgag cctcgtccat aagcatggtt ttggcatctg gatcttccgc
     5461 aaggtgttct cgaacgagtt tgaggaccac caacttatga aaaccaaccg gtccttgcgt
     5521 atgagcgagg tggacttcgg ccatgccgcc gcgtcctatt tcagcaatcg gacggaaccg
     5581 cccgaattcg gcccgagtct ggagcaaggt cgtcatggga ttcacctcgt aggaagtgcc
     5641 ccgtgttctg caacttatgg gccgtcctcc caaccgaagc caaccgttca aacagaaaga
     5701 agatccgata acaccatccc tcaagacgat ttcatgtgtg aaaacggaag gtccgttccc
     5761 tttcacgatc gcgttataat atattacgta tcggtatatt taaccgttgc ccagttcctt
     5821 cgcttctgcc atctctcccc tgccccgttc gccttcctgt tctgttgtcg gtgaaaactg
     5881 ccccttcgtc cccaccgcgt tgaccctaac cgcaattcca aggcatgaac caaccatacc
     5941 atggatgtcg tcacgaacgc caaagtagca tttgtgctcg aacgatatgc cttggacgac
     6001 gtgagcgaaa tttgccttcg cacacgcgcc atggctaccg cgttggcaga acgaggacac
     6061 gacgtcaccg tgctgaccac gtgcagccgt tacccaggac aatggaacaa cgatgtcgcc
     6121 gaaggggagt cgaccatcga ttcgctttcc gtggttcgct tccgtctcga acagcgcctc
     6181 gtgcatcgac tggaccgtcc cgcgaagtgg ctcgcgtcgt acaataccct agcagcgaaa
     6241 gtgtggggcc attcggtggg tccggtctgt ccggggtatc gcccttatct cgaccaattc
     6301 ggacctcttt tcgatgccgt ttttttcacg ggggcgtggg ggtacttggt atccgaaggg
     6361 atgggccgtg tacgtaacgc cgtgctttgt ccggcaccgt gggaggatcg aacccaccaa
     6421 ggaaagcaag ccgcaaccct gcttcggcag gcgcgcgggg tcgcactggc ttcttctgca
     6481 gaagaaaaac gattacgaac cgcgacagag tatccgtggt cttgtccgac tttcgtgatc
     6541 ggtgcggccc ccgatccgct tccttcccca tcttatcgtt cgaagcgact gacgctggtc
     6601 gacggccctt ttcttcttgc gttcggacat tacagccccg cggcggatcg gttgctcgaa
     6661 gcgttccaat tgtttcgtca agcacacggg gatacttcct tcgaagacga ccgttccgaa
     6721 cggatgagcg caaaaaacgt tcggctcgtt cttgcgggtg acgacagatt cccccacgac
     6781 ccaggcaatg gcgtcgtatc gctgggaccg gtggacgata cggctcgcga atccttgatg
     6841 cgccatgcct tggcggtagt gcatgcggat ccctgtattc gattatccac gggcttgtta
     6901 tccgcgtgga tgcaaggatg cccaacgctg attcatgcgg gcagtaccga tttgcagcca
     6961 gtactcgatg cgattggcaa agaatgcgta ttcgactcgg cattgacctt tgcagcaggg
     7021 gccgcttcgt tgctatcgag cccaggtcca cgacgagctt atggcgcgcg tgcgcagcac
     7081 atggttcagc gggtcttttc gccgagaaaa cttggcgaat gcctggagca atgtgttcgc
     7141 gcggtcatgg gacgcgccaa gggtcagcca gtttgatgca tggggattga cccatggtcg
     7201 acagaccttg gtttgtcgct ctgctctttg gtacaagacc tcaccatgac agttctggtt
     7261 gcgggtggag caggctacat cggatctcat ttcgtgcgtt gcctcgaaga gcatggcgag
     7321 tccgcggtgg ttctcgacga tctcagcacg ggtttcgccg aggctatccc tgaccacgtt
     7381 cctctggttc gttgtgccat cggcgatcgc gcggcagtga gatccgtcct cgaagaagtc
     7441 gcccccgaag ccgtcgtgga tttcgctgga ttgattcaag tcggtgaatc gatgcgatcg
     7501 cctgatcgat actatggggt caatgtttcg caaaccattg catttcttga tgaaatagtc
     7561 cgatcgggcg tgaaaacgtt cgtgttttcg tcgagcgctg cggtctacgg aaaccccatt
     7621 cgtgttccca tcgacgaaga tcaccccaag gagcccatca atccctatgg agccaccaaa
     7681 ctcatcgtgg aaatggcggc gagagattat gcgcgagcgt acgggcttcg cgtggcttgt
     7741 ttgcgatact tcaacgcggc cggagcgcat gcggatggta ccctggcgga acgtcatgat
     7801 ccggaaaccc atctcatccc gctcgccatc gatgccggct tgggacgtcg tccggaacta
     7861 acggtgttcg gcgatgattg gagcacgccg gatggcactt gtgtgcgtga ctatattcac
     7921 gtgatggact tggccgaagc gcattggctg gcgatcgaga aacttcgcaa cgataccccc
     7981 acgttgtcgt tgaacctcgg aacgggcaat ggttattcgg tacgtcagat catcgacgct
     8041 gtgagtgatg tgcttggcaa agcggttccc tatcatatcg gaccgagacg cgatggcgat
     8101 cctccttcgt tggtggcgtc atcgaatcgg gctcgcaacg tgcttggatg gaatcccact
     8161 cgctcgcaac tcgatacgat cgtacaagac gccgtgcgca gccggacatg aatcatgatt
     8221 gactgattcg cattcctcgg tgctgctcag ccatccatca tgagtttgga agctgattgg
     8281 ttccgtgaga acagagcttt gacttccggt ttcgaaagct gaatcaac
//
LOCUS       DAOSOR010000213         8319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8530_length_8319_cov_4.643030, whole genome shotgun sequence.
ACCESSION   DAOSOR010000213 DAOSOR010000000
VERSION     DAOSOR010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8319
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8530_length_8319_cov_4.643030"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..563)
                     /locus_tag="PK710_12555"
     CDS             complement(192..563)
                     /locus_tag="PK710_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10599.1"
                     /translation="MDRGEHARVLLIVEDRADVGLAFRRYFSRYFDSVLLASTPAQAE
                     SILQGEPSPTHMICDHWLGEGIPVGLILLSKWRAQFPSLKRCIIVSGSEIEPLSLPPG
                     VDAVFNKPADMSRIREFLLAA"
     gene            complement(634..1899)
                     /locus_tag="PK710_12560"
     CDS             complement(634..1899)
                     /locus_tag="PK710_12560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT10600.1"
                     /translation="MASWWESQWTSFVSSYDAPTAMVQSNPKAILIGIGSLVAGYLAI
                     GYVPAIASLAGLRDPWIPALFAILGAFCSYLAWRHQATGPIGSTCMLLDTALYTSSLT
                     LAAVRSEYPFSLGFSIALALFLLAFPSRVYSLTWLIALATTGPSVVITALWGHDATVA
                     LVVWAACSLAITASYRTGQQRALVQQNLRLRNALGTADQVAEHTMETALAASLLDIGH
                     FLHELRNLRAVEQTNLQFVREEGKLEGDVLEALDDAIAAQDKENALISEAIERLRRKA
                     TPAKKSFSLHDVLSRFVHEQPEGLALTLDFQAPPFQMQGDPRHVLAVLSNLIRNSQRA
                     GATQARIALEATANAKAVALVVEDNGPGLRESQIPELFKPFTSYGEGLGLGLYLTSRY
                     VQLLGGQIEGENRPSGGAVFRILLPGKPV"
     gene            complement(1990..2994)
                     /locus_tag="PK710_12565"
     CDS             complement(1990..2994)
                     /locus_tag="PK710_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10601.1"
                     /translation="MRRQGVPTDALTSQAASVHPPPSFQAAWSSLFVRGTFNDWEATP
                     MHLEGDNEWAVEIVSNTSKTESFTFDVFGDGRVTLGDDDGDQTAIVPAGIDASALNML
                     PIQLSVDGQSGRVSTIYVDEEHATAHVPVCCLSKGSEATLSIDRVVKEQRWKGEVTWV
                     VDGSVDPIVTQLRWVEASLEDMGMLEVEVFSDRWEADHLVTSPYSDVGVYLGDWPAGN
                     LLGHTGNTGEALFYLAPVTVVLRSPYDTGMGRALYLTGASSYLGDWQTATRMVWADGQ
                     RKWQRSLLLGLPFKIMLADWSQGEHISTSEGQWEQGPNRVEEPPQGTSWSEMTVYPIF
                     "
     gene            3132..3872
                     /locus_tag="PK710_12570"
     CDS             3132..3872
                     /locus_tag="PK710_12570"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT10602.1"
                     /translation="MTPHWSDGFFEGPWVPLHLSFRSPGDIARESEAVVAFLDLPTGC
                     SVVDIPCGPGDHAIELARRGFHVTGIDRSEVLLEHAKRRASKAAVQAVFEHGDMRDVR
                     LGKSFDALLCMWGSVGYFDNAGDRAQIETFWNLLRPGGVMLIDISPLEGILLNFEPRD
                     THRVGSMLVTQERSYDGLRQCIDARWTFEEQGQRIVKHTSMRLYTVRQWREFLETIGF
                     EDVQIIDPQTGSAFALGSFHAWIRARRP"
     gene            3872..4765
                     /locus_tag="PK710_12575"
     CDS             3872..4765
                     /locus_tag="PK710_12575"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOT10603.1"
                     /translation="MGIWRKGVVWVALGWVIPGQALADDSPWRVHYGREAALTGVFVA
                     GSLAFSTLEVDTTTRWKHELIPWDESVRGRHSAGASARSDMLAALTVAAPVMAVVGDG
                     MNESAAHRGMIYAESLSVTLLLQSAAKYLVQRPRPYVYSSELRGRDSDDKDSHLSFFS
                     GHSAMVFTAAVAGSFLFGVQSHDEVSRATLWGAELTMASATANLRVRAGKHYFSDVIV
                     GSVVGAAVGASLPRLYLDNVNSYRPSEVEVGAMVGGVVVGTASAWLIPVPQDVSMDCA
                     GMYWMPMGLPTGLGWSVVGIF"
     gene            complement(4770..5426)
                     /locus_tag="PK710_12580"
     CDS             complement(4770..5426)
                     /locus_tag="PK710_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10604.1"
                     /translation="MTTIRRAAPHILVALSLAIGAAGWMTARMLRQGELELRTAQALA
                     ATGELEQATIHARKAASYFVPNAPHVPAAYAQLISIAQLAEGRGDTQTALFAWNAVRT
                     AAYSSRWISVPHQQEVAIADASIARLTSRQPVPYGANQDPDARQKKMLDLLSRQNYPR
                     MPWVFALLGGFVAVSVGLLHIGWHGLNHPKANTLPRLRVGIALTAFGLVAWALALWNA
                     "
     gene            complement(5423..6355)
                     /locus_tag="PK710_12585"
     CDS             complement(5423..6355)
                     /locus_tag="PK710_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT10605.1"
                     /translation="MLQRISTIALNTYREAARARILYGLVGIALATAMYAVVVGAYTL
                     RSAPRVVSDLGGASISLYSIIVAIVLAATSLHRELEYKTIFPILARPVRRSEYIVGKT
                     LGNLLTLAVFVAIDGFVVLLTLAAMSGRSVPLIVGIGLGSIAVLAFLVFKLRSLGTFL
                     PIPWAFTMLVIASWLASPYPDERRVVLGMCLLTLFEASIVTSIATVFASFSSPFLTAV
                     LTLGVFVVGRQADSLAQLPVKVFGQAIHDVGVFLSKIVPNLHVYVPARPLLTGEAVDS
                     HLGPYFAMSGLQSLGWTVGLLTFSILIFQKRDFL"
     gene            6426..>8319
                     /locus_tag="PK710_12590"
     CDS             6426..>8319
                     /locus_tag="PK710_12590"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HOT10606.1"
                     /translation="MFRLLEIRCRKREQMVIKIGSSTIGILCVGLGVACSAGGGDGPG
                     GGNQAAAGSSGAGGTTPWAGGSGGGESSLYCTPGAWSCSGKTYYLCGQDGLTHEQETQ
                     CPQACKAGVGCVACESGTRSCDGTVSMVCAADGSGWIPGRDCADWGTTCGTSGFCDDA
                     CAQAERTDSYVGCEYWPTPLANTSELSYDVFDYRVVVGNPNTSKAHVIVTRGSATAWE
                     GDVSAGGLVEIPLPWIDGQSFGIPGDAWKSIAVMDGAYRLRSDQPVTVAQFNPFEYSA
                     GGTFSYTNDATLLLPSHVLTGDYINLTYVPLSLTRGATGMVPTPPSSSKFADYLAVVG
                     VSPGVTNVRIQVSSHTAGEQSNRFGPTGPAGWIEFSVQRGEVVHVAAAPPANCAQGRP
                     GYSREEDCFANICDYQDTCFEHEHDMTGSRVVADRPVQVFGGHVCAYVPYMSQACDHL
                     EVQVPPIQTWGKQYVSRPMTDGAISGQNLVRVVAAFDGTEVTVSPPQGGIDKIVLDAN
                     RWVEFMASGPFSVSGTNAIMVGQFLLGQSFPDPEAQRGDPAMTVLVPFEQYRKDYIFV
                     TPSSYSQEKNGQNYLLIIRPPGMALSLDGSAVTATWEAMGGREVGIVAVDGGTHTITG
                     ESAFGMI"
BASE COUNT         1755 a         2159 c         2580 g         1825 t
ORIGIN      
        1 tggaggaacc cctaacgctt gggctagagc gatatttggt ttttttcttt ttctcggatt
       61 tacgcttgcg atttcttttg atattctggc aattttggtg gcctcgctcc gtaagctcgg
      121 gaggctccat cggggccatt gatccaatga agtagtgatt tggaggtggt tcggacagcg
      181 aacaggggcg atcaggcagc gagcaagaac tcacggatcc gggacatgtc ggcgggtttg
      241 ttgaacaccg cgtccacgcc cggaggcaag ctgagcggtt cgatttcgga gccagacacg
      301 atgatacagc gtttcaggct tggaaattgc gctctccact tcgacagaag gatgagtccc
      361 accggaatgc cttccccgag ccaatggtcg caaatcatat gggtgggcga tggctctcct
      421 tgcagaatcg actcggcttg cgcgggtgta gaggcaagca gcacggaatc aaagtagcga
      481 ctgaaatagc gccgaaacgc caagcctaca tccgctcgat cctcgacgat aagcaatacc
      541 cgtgcatgtt cccctcgatc catgattctc caaatatacc agccccactt cgatattcaa
      601 atcgatattt caggtttctg gatgaccctg gattcaaacc ggtttgcccg gtaggagtat
      661 acgaaaaaca gccccgccac tcggtcgatt ctcaccttca atctgtcctc ccaacaattg
      721 gacatagcgg ctcgtcaaat acaaccccag ccccaatcct tccccgtagc tcgtaaatgg
      781 tttgaataat tcaggaattt ggctttcccg caatccgggg ccattgtcct ccaccactaa
      841 cgccacggct ttcgcattgg ccgtcgcctc cagggcaatt cgagcctgtg tggccccagc
      901 ccgctgtgaa ttgcgaatga ggttgctcaa caccgctaga acatgccgag ggtcaccttg
      961 catctgaaaa ggcggcgctt gaaaatcgag cgtcaacgca agcccctcgg gctgttcatg
     1021 gacgaaacgg gatagtacat cgtgaaggga aaagcttttc ttggctggag ttgcttttct
     1081 tcgcagacgc tcgatcgctt ccgaaatgag agcgttttcc ttgtcctggg ctgcaatggc
     1141 atcgtccaag gcttcgagga catcgccttc gagctttcct tcttctctca cgaattgcag
     1201 attcgtctgc tcgaccgcgc gcagattccg caattcgtgc aaaaaatggc cgatatcgag
     1261 caacgacgcc gccaaagccg tttccatcgt gtgttccgcg acttgatccg cggtgccaag
     1321 tgcatttcga agacgaaggt tctgctggac caacgcgcgt tgctgacccg ttcgatacga
     1381 tgcggtgatc gccagagaac aagctgccca tactaccagg gccaccgtcg catcatgccc
     1441 ccacagcgcg gtgatgacca ccgatggccc ggttgtcgcc aacgcaatca accaggtcag
     1501 agaatagaca cgcgaaggaa acgcaagcaa aaatagggct aaagcgatag aaaatcccag
     1561 cgaaaacggg tattcggaac gaacggcggc gagggtcagc gacgatgtat acagcgccgt
     1621 gtccaacagc atgcacgtgc tgccgatcgg acccgtggct tgatgacgcc atgccaggta
     1681 ggaacaaaac gctcctaaaa tagcaaatag ggcgggaatc caaggatctc gaagcccagc
     1741 cagcgatgcg attgccggaa catatccaat cgcaagatat ccggccacaa ggcttcctat
     1801 cccaatcagg atggccttgg gattggattg caccatcgcc gtcggggcgt catacgacga
     1861 tacgaacgat gtccactggc tttcccacca gcttgccatg aatcctatgg taataaccaa
     1921 gcacgacgcg cttgtccatg cccactcatg aactccgaca ggcttattct ttccgtacgg
     1981 taaaaacgct caaaaaatcg ggtataccgt catctcgctc cacgaggtgc cctgcggtgg
     2041 ttcctccacg cgattcggcc cctgctccca ttgcccctca ctcgtcgaga tgtgttcgcc
     2101 ctgcgaccaa tccgccagca tgatcttgaa agggaggcca agcagtagac tccgctgcca
     2161 cttccgttgg ccatcggccc aaaccatgcg cgttgccgtt tgccagtcgc ccagataaga
     2221 actggccccg gtgagataaa gggcgcgacc cattcccgta tcgtaaggcg agcgaagaac
     2281 aaccgtgacc ggggcaagat agaacaatgc ttcgccggtg tttccggtat gcccgagcag
     2341 gtttccggcc ggccagtccc caaggtacac accaacgtcc gaataaggag aggtaaccag
     2401 gtggtctgcc tcccaacgat ccgaaaacac ctcgacttca agcatcccca tgtcctcgag
     2461 ggaggcttcg acccaccgaa gctgcgttac gatcgggtcc acactaccat cgaccaccca
     2521 cgttacttcc cccttccagc gttgctcctt caccacacga tcgatagaaa gtgtcgcttc
     2581 cgatcctttc gatagacagc aaaccggcac atgggcagtt gcatgctcct catccacata
     2641 aatggtgctt acgcgaccag attgtccatc gacggatagt tgtattggca gcatgttcag
     2701 ggccgatgca tcgatgcccg cggggacaat cgctgtctgg tcgccgtcgt catctcccaa
     2761 ggtcacgcgc ccgtcaccaa aaacatcgaa cgtgaagctt tcggttttgg aggtgttcga
     2821 aacgatttca acagcccact cgttgtcccc ttccaggtgc atgggtgtcg cttcccagtc
     2881 gttgaaagta cctcgaacga aaaggctgga ccatgcggcc tgaaaggatg gtggtggatg
     2941 cacactcgct gcctgggaag tgagcgcatc ggtggggacc ccttgacgcc gcaagccatc
     3001 aagcaagcta gcagcccaac gaggatagcg atacgagaga gaagagatct aacgtacctc
     3061 ggttgtgagg cacgttcgat atgtactgtg ccacattgtt tgtcaacgag gatttgttta
     3121 cattcaaccc catgacgccc cattggagcg atggtttttt tgaaggcccg tgggtgccgt
     3181 tgcatttgtc cttccgttct ccgggggata ttgcgcggga gtcagaggcg gtggtggctt
     3241 ttctcgactt gccgacggga tgttcggttg tcgatattcc ttgcggtcca ggggatcacg
     3301 cgatcgaact ggcgcgtcgg ggttttcacg tcaccgggat cgatcgctcc gaagtcctgc
     3361 tggagcatgc gaagaggagg gcgtcgaaag cggctgtgca ggcggttttc gagcatgggg
     3421 atatgcgcga cgttcggttg ggaaagtcat tcgacgcgct tttatgtatg tgggggagtg
     3481 tgggttattt cgacaacgcg ggggatcgcg cccagatcga gacattttgg aatttgcttc
     3541 gtcctggggg ggtgatgttg attgatattt cacctttgga ggggattttg ttgaatttcg
     3601 agccgcgcga tacccatcgt gtaggctcga tgttggttac ccaagagcgg agctacgatg
     3661 ggttgcggca gtgcatcgac gcccggtgga cctttgagga acagggccag cgcatcgtaa
     3721 aacacacgtc gatgaggcta tataccgtgc ggcaatggcg tgaattcctt gaaaccattg
     3781 gcttcgaaga cgtgcagatc atcgatccgc agaccgggag tgcgtttgct cttggctcgt
     3841 ttcacgcctg gatacgggca aggcgacctt gatgggaatt tggcgaaaag gagtcgtttg
     3901 ggttgcgttg gggtgggtca tcccggggca agcgctggcc gacgattccc cctggcgtgt
     3961 ccattacggg cgagaggctg ccttgactgg cgtttttgtg gcgggctcgt tggctttctc
     4021 aaccctcgag gtggatacga cgacccggtg gaaacatgaa ttaataccgt gggatgaatc
     4081 ggttcggggg cgtcattcgg cgggggcttc cgcgcgaagc gacatgcttg ccgcgttgac
     4141 ggtggcggcg ccagtgatgg ctgtcgttgg cgatggaatg aacgaatccg cggcgcatcg
     4201 aggcatgatt tacgccgaat cgttatccgt gaccctattg ttgcagtcag cggcgaagta
     4261 tctggttcag cgtccaagac cctatgtgta ctcctccgag ctgcgcggga gggacagcga
     4321 cgacaaggac tcccatctat cgtttttttc ggggcattca gcgatggttt ttaccgcggc
     4381 ggtggcgggc tcctttcttt ttggtgttca gagccatgat gaagtctccc gtgccacgtt
     4441 atggggggca gaactcacga tggcgagtgc gacagcaaac ctgcgtgtcc gagcagggaa
     4501 gcattatttc tcggatgtga tcgtcgggtc tgtcgtaggc gctgctgtgg gcgcgagttt
     4561 gccacgatta tacctggaca acgtgaactc ctaccgtcct tccgaggtgg aggtcggggc
     4621 gatggttggg ggagtggtgg tgggaacggc gtcggcgtgg ttgattccgg ttccgcaaga
     4681 cgtttccatg gattgcgctg ggatgtattg gatgccgatg ggcctgccga cggggttggg
     4741 ttggtcggtg gtggggatat tttgacagtt taggcgttcc acagggcgag ggcccaggca
     4801 acgagaccga aagcggtgag ggcgataccg acacggagtc ttggcaaggt gttggccttg
     4861 gggtggttga gtccatgcca tccgatgtgc agcagaccga cggatacggc gacaaatccg
     4921 ccgagcaacg caaataccca gggcatacgc gggtaatttt gacggctgag caagtcgagc
     4981 attttttttt ggcgtgcatc cgggtcctgg ttggctccgt aggggacggg ttgtcgagaa
     5041 gtcagtcttg cgatggaggc atccgcgatg gcgacttctt gctggtgggg gacagaaatc
     5101 cagcgactgg agtaggcggc ggttcgaacg gcattccagg caaaaagagc ggtttgggta
     5161 tcgcctcttc cttcggcgag ttgcgcgatg gagatgagtt gggcataggc ggcggggacg
     5221 tggggagcgt tcggaacgaa gtaggaagcg gcttttcttg cgtgaatggt ggcttgttcc
     5281 agctctccgg ttgcggcgag ggcttgtgcg gtacgcagtt cgagttcacc ctgtcgcagc
     5341 atgcgcgcgg tcatccaccc tgcggcgccg atggcgaggc ttagcgccac gaggatatga
     5401 ggggccgcgc gtcgaatcgt agtcacagaa aatcacgttt ctggaagatg agaatcgaaa
     5461 aggtgagaag cccgacggtc cagccaaggc tttgcagtcc ggacatcgcg aagtacggac
     5521 caaggtgact atcgaccgct tctcccgtca gcaagggcct ggcaggtacg tatacgtgca
     5581 aatttgggac gattttcgat agaaatacgc caacgtcatg aatggcctga ccgaatacct
     5641 tgacgggaag ttgcgcaaga gaatcggcct gtcgaccgac gacgaagacg ccaagggtta
     5701 gcacggcggt caaaaacgga gatgaaaacg aagcgaaaac ggtggcgatg ctcgtcacga
     5761 tcgacgcttc gaaaagggtg agcaaacaca taccgagcac gacgcgtcgc tcgtcggggt
     5821 agggggaggc gagccacgat gcgatgacga gcatggtaaa cgcccatgga atgggcagga
     5881 acgtaccaag ggacctgagt ttgaacacca gaaaggccag cacagcgatg gaaccgagtc
     5941 cgatgccaac gatcagtggt acggatcttc cgctcatggc ggcgagggtg agcagcacga
     6001 cgaagccatc gatggcgacg aatacggcga gggtaagcaa gttgcccagg gtttttccca
     6061 cgatgtattc ggaacgtcgc accgggcggg cgaggatggg aaagatggtt ttgtattcga
     6121 gttcgcgatg gagggatgtt gccgcaagaa cgatagcgac gatgatggaa tacagagaga
     6181 tggaagcgcc accgaggtcg gagacgacac gcggtgcgga gcgaagggtg taggctccca
     6241 cgacgacggc gtacatggcg gttgccaggg cgataccgac gaggccgtac aggattctcg
     6301 cgcgtgccgc ttcgcgatag gtgttcaacg caatcgtact gatccgctgc agcatggctt
     6361 tggttgttgg taagccgact tgagctttgg tgcaaccttt gtggtatgtt caacgcgagt
     6421 gcggtgtgtt tcgcctgctt gaaatccgtt gccgaaagcg tgaacagatg gtgatcaaaa
     6481 ttggttcgtc gacgattggg atcctttgtg ttggtttggg cgtagcttgc tccgcgggtg
     6541 gcggggatgg ccctgggggc gggaatcaag ccgccgctgg ctcttcggga gctggaggga
     6601 caacgccttg ggctggaggc tctggtggag gagagtcgtc tttgtattgc accccggggg
     6661 catggtcttg ttccggtaaa acctattatt tatgtgggca ggacggactt acccatgagc
     6721 aagaaacgca atgtccccag gcctgtaagg ctggggtggg atgtgtggct tgtgagtcgg
     6781 ggacgcgctc ttgcgatggc acggtgtcga tggtttgcgc tgccgacgga tccggttgga
     6841 taccgggcag ggattgtgcg gattggggga cgacatgcgg tacatcggga ttttgcgatg
     6901 acgcgtgtgc gcaagcggag cgaacggatt cgtatgttgg gtgcgaatac tggccgacgc
     6961 ccttggccaa tacctcggag ctttcgtacg atgtattcga ttatcgggtg gttgtaggta
     7021 atccgaatac ttccaaggct catgtgattg tgacgcgggg tagcgccacg gcatgggaag
     7081 gggatgtttc cgccggtggg ttggtggaga taccgcttcc ttggatcgac ggacagtctt
     7141 ttggaattcc gggggatgcg tggaagagca ttgcggtgat ggacggcgcg tatcgtttgc
     7201 ggtcggatca accggtgacg gtggcgcagt tcaatccttt cgaatacagc gccgggggaa
     7261 cgttttccta tacgaatgac gccaccttgc tgctgccttc ccatgtgttg acgggtgatt
     7321 atatcaactt gacgtatgtg cctctcagct tgactcgggg cgcgacagga atggttccca
     7381 ccccgcccag ttcctcaaaa ttcgcagatt atttggctgt tgtgggtgtt tccccaggag
     7441 tgacgaacgt acgcattcaa gtgtcgtcgc atactgcggg ggaacagagc aaccgttttg
     7501 gtccgacggg tccggctgga tggattgagt tttcggtgca gcgtggggag gtggtccatg
     7561 tggccgcggc accgccagcg aattgcgcgc aaggccgccc cggctattcg cgagaagaag
     7621 actgtttcgc gaatatctgc gactaccaag acacctgttt cgagcacgaa cacgatatga
     7681 caggcagtcg ggtcgtagcg gatcgtcctg tgcaggtttt cggtggacac gtatgtgcct
     7741 atgttccgta catgagtcag gcctgtgacc acctggaggt ccaggttccg cccatccaaa
     7801 cctggggcaa acaatatgtc agccgcccga tgaccgatgg tgcgatctcc ggtcaaaatc
     7861 tcgtgcgtgt ggtagcggct tttgacggca ccgaggtgac cgtcagtcct ccgcaaggtg
     7921 ggatcgacaa aatcgttctc gatgccaacc gctgggtgga gttcatggcg tccgggccgt
     7981 tcagcgtctc cgggaccaac gcgatcatgg ttgggcaatt cttgctcgga caatcgttcc
     8041 cggaccccga agcgcaacga ggggaccccg ccatgaccgt gctggttccc ttcgagcaat
     8101 accgcaaaga ctacatcttc gtcacaccat cctcgtacag ccaagaaaag aacgggcaaa
     8161 actacttgct catcattcgt ccgccaggca tggctctgtc gctcgacggt tctgctgtca
     8221 ctgccacctg ggaggccatg gggggacgag aagtgggcat cgtggctgtc gatggcggaa
     8281 cgcataccat cacgggtgag tcggccttcg ggatgatcg
//
LOCUS       DAOSOR010000214         8292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8571_length_8292_cov_4.566590, whole genome shotgun sequence.
ACCESSION   DAOSOR010000214 DAOSOR010000000
VERSION     DAOSOR010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8292
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8571_length_8292_cov_4.566590"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PK710_12595"
     CDS             complement(<1..538)
                     /locus_tag="PK710_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10607.1"
                     /translation="MGSWIVRAVVGALLVATGGCSANDSDGVNSAGGSGKTDGGGGSG
                     GGSGVDGGGGTFYIDASNDSPPPTLGCSADLQNVIDANGTVLESCAPSQGCAGGKCVA
                     PCEAAAASQGNVGCDFVVPTPSFMPLITQPCFAVFIANNWPRPVQINVTYAGQSLPVT
                     QFARIPSTNPNPASWESVP"
     gene            complement(679..2256)
                     /locus_tag="PK710_12600"
     CDS             complement(679..2256)
                     /locus_tag="PK710_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT10608.1"
                     /translation="MSWKSIVFSSGKRVLETGPILRWMSDDRVMRVANTVMDAPGRVR
                     AAAGKAAEAWQVLIHGYDLPEMDPSMMPDDAMMARPAKPSKVVKPTREEGPEEESSAA
                     TVDEQKLADQIAERTSLAGQAGRDVFQKCYDFKAADNARKMGVYPYFRPLDFNDGPEA
                     VLEGRRVLMLGSNNYLGLTTHPKVREAALEAIRKYGTSMTGSRLVNGSMKLHKEFEEK
                     LAAFHGKEAALVFTTGYQVNIASLSALLSNKHTVALIDRSDHASIYDGVRLGVAVGAR
                     MLRYKHNDPESLDRQLESLDENTGAFVVTDGVFSAEGEIANLPELVKVAKKHRARIMV
                     DDAHALGVIGPVGRGTAAYFDIEKDVDLIGGTFSKSLASIGGWLVGERKVLEYIQHFA
                     SSFMFAASAAPPNVAAAMAALEVMQEESWRIDDLRKNFTFMREELIKMGWDVGHSQTA
                     VIPLYVRKDLRTIMVWKELLDNYGVYTNPFISPGVPPKQACLRTSYMATHTQEQLERA
                     LEAFEAVGKKFGILGKS"
     gene            complement(2454..3173)
                     /locus_tag="PK710_12605"
     CDS             complement(2454..3173)
                     /locus_tag="PK710_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10609.1"
                     /translation="MKRFDRHGGWCAGLVLLVSGIAVSSLAIAQGQHAAAGSGSASAR
                     PNVGYAPDPPPIRSASQWLLTLQYQRGKVRLLDARRVVLARPAVTARRMGRYAVELLS
                     GPTVIERVRFEFPLIGADELAGKPRPMQAPPSFESKATVTYRVMLPDTPRASRARFVD
                     RATGESFMIPWPPIRPASVPGRHQDAGKDHIGDEKADAHEADGGTTDGDKRRDGGGPD
                     GARDANFHRDIGSLDVIPLDS"
     gene            complement(3221..4162)
                     /locus_tag="PK710_12610"
     CDS             complement(3221..4162)
                     /locus_tag="PK710_12610"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOT10610.1"
                     /translation="MAERTHSSRWTVEFACSLDHVLARMSGSAEALRDGRVFVDGKRV
                     FDPLLPLEPGAFVDVFPAPPEQPRNLHVLSTHENLIAVMKPAGLSTIPDQRGHAASLL
                     DAVAKALGERDLSRVHPTSRLDRGVSGVVVFALGKQAREIMQQARQQNRYQRHYVALV
                     AQPPTPSQGKIDAPIGRGKRPTERSVHGKNAVNSITYFQTIDRLPQMAMVAVEPQTGR
                     THQIRVHLSHANAPLLGDDTYGGPRRLVLPRGAVLRFDRVALHAAWVKIAWPDGTPWH
                     VEAPMDEVLMRWWSRAGGHPDSWNRAWQPLSVDMGST"
     gene            complement(4164..6788)
                     /locus_tag="PK710_12615"
     CDS             complement(4164..6788)
                     /locus_tag="PK710_12615"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOT10611.1"
                     /translation="MTQTVWLYPIENAPIDPAEVGGKGMRLAWLHRHGFRTPRGWILP
                     ATVFSTTIASQIPSDLHPRALIQSMNRADALSQIGRARERWLSAPLPPSLVQELEFLW
                     ETVSADAPWGLAVRSSATCEDSELTAMAGLATSVLGVRGVDGLIHAIREVWASAFLPR
                     SLQHLAFRGIRDLAMGVVLQVMVRAEASGVMFTRPPAGTDDSVWASDERLINATLGLG
                     APVVNGAVVPDVVRLRKNTRAVVHQTIAVKDRSLMIGDHGPDYVSVPLPRACEPALST
                     VALHELSSLADALEQVENNAFDVEFAVQNDEVFLVQARPIVGSGFPPGGDENTVWSRA
                     NVGEALPGVATPLTWSVAQDFSERGFHQAFRQLGCVVPRGATMVANIHGRFYLNLTSF
                     MRIAAQVPGLRPERFVDLGGGTTVPQLEDQIRDVSHAGFLRRLPITASRVWIERTQLA
                     SRVARFERSAGVRHRAWTASALQQLDDRRLANAFRDVLQQLDHTGTLMLACASSSLGS
                     HLALEALLRLLSVSEPQTTAQSLTAGVDELESAKPGMAIAKIASLVRRNQLIKQGILS
                     ASYQRIEDIPQGPVRHELLRFVEDFGDRAVREAELATARWAEDHSQIMGMLAVALHNP
                     DSQERAGPLRARRRADHTMARLERELPWWKASAIRTLVGQARQTMILREKMRAWVTRC
                     LGAIRRVALEIDCRLRKQDPRLEPGSAFFCTTQELMAAMDKGSMDLFQIVQMRRAEYA
                     RDERRADPPVTFVGRPRSVVLPPAGKEWLRGLGASPGVVEGKVRLLAAGNELKEGIEP
                     GEILVARTTDVGLTPLFLLAAGVVTELGGPLSHAALVAREYGVPAVVNVAGVTVALRT
                     GERIRIDGDRGIVERLEA"
     gene            complement(7004..8164)
                     /locus_tag="PK710_12620"
     CDS             complement(7004..8164)
                     /locus_tag="PK710_12620"
                     /inference="COORDINATES: protein motif:HMM:NF024151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10612.1"
                     /translation="MRYIAWSVVFGLALIGCGSSTAESLLEPSSVQADSGTQDVAPDI
                     TADQAAEATDHPDVSDDVIVDVALEVASDIAPEVVDEPVETVPDPMVSPDVPVTEWKG
                     KLSPGSMVSKEMVGFRPTADPPSGELYPLVVFARDHQAQKDSYKKTMKHIAQFGYVTA
                     SVDYDLGLLDPDHHAPVENLKRAIDVLTTKPPDNVGNVVDPKRIAAMGHAVGGKAAVW
                     LALEDPRVSAVITLSPVDDDPGSLVGSSHRPSLAPEQMGKFKVPALFLGAALGSSSNS
                     CAPSASNACRFFESVPDKTTAWLGVLEAFGHLQFIDDYDCLLLCGSCPRGPKAEHEKR
                     QLVFRGLSVAFLQLQFRHASGYLSWLEGTERTSLETANFLLDTAEQGNFCKP"
BASE COUNT         1727 a         2457 c         2437 g         1671 t
ORIGIN      
        1 cggggacgct ttcccaggac gctggattcg gattcgtgct gggaatgcgt gcgaattgcg
       61 tgacgggaag agactgcccc gcgtacgtaa cgttgatctg gacaggcctc ggccagttgt
      121 tggcgatgaa tacggcgaaa cagggttgcg taatgagggg catgaacgat ggtgtgggaa
      181 cgacgaagtc gcagcctacg tttccttggc ttgccgcggc agcttcgcag ggggctacgc
      241 attttcctcc cgcgcatcct tgcgatgggg cacagctttc gagcaccgtg ccgttggcat
      301 cgatgacgtt ttgcaggtca gcactgcatc ctaacgtggg aggtggagag tcattgctgg
      361 catcgatgta aaacgtgcca ccgccgccat ccacgcccga tccgccgccg gaaccgcctc
      421 ccccatcggt tttccccgac cccccggccg aattcacacc gtccgaatca ttggcagaac
      481 aacccccggt ggctaccaac agggcaccca ctaccgcacg cacgatccat gaacccattt
      541 gtcacctcac cgcgaacgag tctccatgtc gattgtagcg caggggcggg cgagcaacgt
      601 ggatagaatg cacagacggc ggcgtccgtg gatcctccgg atcggcgctg tcgaaaattt
      661 tctagtaaat ctgatgggtt acgacttgcc caggatgccg aatttctttc ccacggcctc
      721 gaacgcttcg agcgcgcgtt cgagttgctc ttgggtgtgg gtcgccatat agctcgtgcg
      781 aagacaagct tgcttgggtg gcaccccagg actgatgaag gggttggtgt atacgccgta
      841 gttgtcgagc aactccttcc acaccatgat cgtgcgaaga tctttgcgta cgtataaggg
      901 gatcaccgcc gtttgactat gccccacatc ccatcccatc ttgatgagtt cctcacgcat
      961 gaacgtaaag tttttgcgta ggtcgtcgat gcgccaagac tcttcttgca tcacttccaa
     1021 cgccgccatg gccgccgcca cattcggtgg tgccgcactc gccgcaaaca tgaaggatga
     1081 tgcaaaatgt tgaatatatt caaggacttt tcgttcaccc accaaccagc cgccaatcga
     1141 agccagggat ttggagaacg ttccgccaat caagtcgaca tccttttcga tatcgaaata
     1201 agccgccgtg ccgcgcccta cgggaccaat gacccccagc gcatgcgcat cgtcgaccat
     1261 gatgcgcgcg cgatgttttt tcgccacctt gaccagctcc ggtagattcg cgatctcgcc
     1321 ctctgccgaa aataccccat cggtgacgac gaacgcgccg gtgttttcgt cgaggctttc
     1381 gagttgacga tccaacgact cggggtcgtt gtgtttgtag cgaagcatgc gcgcgccaac
     1441 ggccacaccc agtcggacgc catcgtagat ggaggcgtgg tcgctgcgat cgatcaaggc
     1501 gacggtatgt ttgttggaaa gcagggccga caaggaggcg atattgactt ggtaacccgt
     1561 ggtgaacacg agggctgctt cttttccgtg gaaagcggca agcttctctt cgaattcctt
     1621 gtgcagtttc atcgagccgt tgaccaggcg cgaccccgtc atgctcgtgc cgtacttgcg
     1681 gatggcttcg agcgctgctt cccgcacttt cggatgcgtc gtcaaaccaa ggtagttgtt
     1741 ggagccgagc atcaacacgc gacgtccttc caacacggct tcggggccat cgttgaagtc
     1801 cagagggcgg aagtaaggat acacacccat cttgcgcgcg ttgtccgcgg ccttgaaatc
     1861 gtagcacttc tggaagacgt cgcgacccgc ctgaccggcc agcgatgtac gctccgcgat
     1921 ttgatccgcg agcttctgtt catccaccgt cgccgcggat gattcttctt ccggcccttc
     1981 ctctcttgtt ggctttacga ctttggaagg ctttgctgga cgcgccatca tggcgtcgtc
     2041 gggcatcatc gatggatcca tttcgggaag gtcgtagcca tggatcaaca cttgccaggc
     2101 ttccgctgct tttccagccg ccgcgcgaac acgacccggc gcatccatga ccgtattggc
     2161 cacgcgcatg acgcgatcat cactcatcca tcgaagaatg ggaccggttt ccaatacgcg
     2221 tttgccagaa ctaaagacga tgcttttcca actcatgaag cttatccatt gagtgagtgg
     2281 ggcggcatgg ccaaccacga cgatccacga ttcagcggcg ggaagctagg caacggtgtg
     2341 ggcggctgtc aatcgaggca acggatgttg tcgtgatcgt cgtaagatcg gaagtcaggt
     2401 gcaaaaacca tttcgcagcg agcaggatcg atatcgacag cccatggcct cgtttacgaa
     2461 tcgaggggga tcacgtccaa cgagccaata tcccgatgga aattcgcatc ccgcgcccca
     2521 tccggtcctc caccgtccct tcgcttatcc ccatcggtcg tgcccccatc cgcctcatgc
     2581 gcatcggcct tctcgtctcc aatatgatct tttcccgcgt cttgatgacg cccaggcacc
     2641 gacgcgggcc gaatcggcgg ccaaggaatc atgaagcttt ctcccgtcgc ccggtccacg
     2701 aatcgagcgc gcgatgcacg aggcgtgtct ggtaacatca cccgatacgt caccgtggct
     2761 ttgctttcga aactcggtgg ggcttgcatc ggtcgcggct tgcccgcgag ttcgtcagca
     2821 ccgatcaaag gaaactcgaa acgcacgcgc tcgatgaccg tcggcccact gagcaattcg
     2881 accgcgtacc gtcccatgcg acgagcggtc accgcgggtc gagcaagcac aacccgccgc
     2941 gcatcgagca gccgaacctt tcctcgttgg tattgcagcg tgagcaacca ttgagaggcc
     3001 gagcgaatgg gcggagggtc gggcgcatat cccacgttgg gccgcgcgct cgcgctaccg
     3061 gagccggccg ccgcatgctg cccttgggcg atcgccaacg acgaaaccgc aattcccgaa
     3121 acgagcagga ccaaccctgc acaccaccca ccatgtcgat cgaaacgctt catcctttga
     3181 tcccgcaagt cagcacgcaa agcttcccac gatcgctgtg ctacgtcgag cccatgtcga
     3241 ccgacaaggg ttgccacgca cggttccagc tatccgggtg accacccgct cgagaccacc
     3301 atcgcatcaa cacttcatcc atcggagctt ccacatgcca aggagttcca tcgggccaag
     3361 caatcttcac ccacgccgca tgcagcgcaa cgcgatcgaa acgtaggacc gctcctcggg
     3421 gcaatacgag ccttctggga cccccatagg tatcatcccc aagcaacggc gcgttggcat
     3481 gagaaagatg aacgcgaatc tggtgagtgc gcccggtttg cggttcgacg gccaccatcg
     3541 ccatctgcgg cagtctgtcg atggtttgaa aataagttat tgaatttaca gcattttttc
     3601 catgaacgct ccgttccgtg gggcgtttcc ctctgccgat gggcgcgtcg attttgccct
     3661 gcgatggtgt tggcggttga gcaaccaacg ccacgtagtg acgttgatag cggttctgct
     3721 gtcgcgcttg ctgcattatt tcgcgggcct gcttgcccag tgcgaagacg acgacgccgc
     3781 tcacgccacg gtcgagccga gaggtaggat gcacgcggct gaggtctcgc tctcccaacg
     3841 ctttcgcgac agcatcgagc aaacttgcgg cgtgtcctcg ctgatcggga atcgtcgaca
     3901 ggcccgcggg tttcatgacg gctatcaagt tttcatgggt cgatagcacg tgcaggttcc
     3961 ggggttgttc gggcggcgca gggaacacgt ccacgaaagc acccggttcg aggggcaaga
     4021 gcggatcgaa tacgcgtttc ccatccacga atacgcgacc atcgcgcagg gcttcggccg
     4081 acccgctcat gcgcgccagc acgtgatcaa gcgagcaagc aaactcgaca gtccagcggg
     4141 aagaatgggt gcgttcagcc atgtcatgcc tcgaggcgct cgacgattcc tcggtcgcca
     4201 tcgatgcgaa ttcgctctcc cgtacgcaag gcgacggtga ctccggctac attgacgacc
     4261 gcgggcacac catattcgcg tgcgaccagg gctgcatggg aaagggggcc tcccaattcg
     4321 gtgacgacgc ccgcggcgag taaaaaaagc ggcgtgagac caacatcggt ggttcgagcc
     4381 accaagattt ctcccggctc gatcccttct ttcagttcgt ttcccgcggc aagcaaacgg
     4441 actttgcctt cgaccacgcc agggcttgcg ccaagtccgc gcaaccattc ttttcccgcg
     4501 ggaggcagca caaccgaccg cggacgtccc acgaacgtga cgggggggtc cgcacgacgt
     4561 tcgtcgcgag catattcggc gcgacgcatt tgtacgatct gaaatagatc catgctcccc
     4621 ttatccatcg ccgccatgag ttcttgcgtc gtgcaaaaaa aagcagatcc gggttcaagc
     4681 cgtgggtctt gtttgcgtaa ccgacagtcg atctcgagag cgacccttcg aatggctccc
     4741 aaacaacggg ttacccaggc gcgcattttt tcgcgcagta tcatggtctg gcgtgcctgc
     4801 ccgaccaacg tgcgaatcgc agaggctttc caccacggca gctcacgctc gagcctggcc
     4861 atcgtgtggt ccgcacgcct tcgcgcgcga agggggcccg ctcgttcctg cgaatccggg
     4921 ttgtgcaatg cgaccgcaag catccccatg atttgggaat gatcttccgc ccaacgagcc
     4981 gtggccagct ccgcttctcg tactgcacga tccccaaaat cctctacgaa tcgaagcaac
     5041 tcgtggcgaa cgggaccttg gggaatgtct tcgatccgct ggtaggaggc ggataaaatc
     5101 ccttgtttta tcaattggtt acgacgcacg agggaagcga ttttcgcgat ggccatgcct
     5161 ggctttgcgc tttccaattc atctacaccc gctgtcaagg attgcgccgt ggtttgcggc
     5221 tcgctcacgg acaaaagccg aagcagcgct tcgagcgcca aatgactgcc gagcgacgac
     5281 gaagcgcagg cgagcatcaa ggttcccgtg tggtcgagct gttgcagcac atcgcgaaac
     5341 gcgttggcca agcgacgatc atcgagttgt tgcagcgccg aggcggtcca tgctcgatgt
     5401 cgaacgccag cacttcgttc gaatcgagct acccgagagg ccagttgtgt tcgctcgatc
     5461 catacccgtg atgcggtgat gggcaaacga cgcaaaaagc cagcgtggga gacatcgcgg
     5521 atctggtctt cgagctgggg caccgtcgtg cctccgccca gatcgacgaa cctttcgggg
     5581 cgcagtccgg gcacctgggc ggcgatacgc atgaacgagg tgaggttgag gtaaaagcgt
     5641 ccatggatgt tggcgaccat cgtggctccg cgggggacaa cgcatccaag ctggcgaaat
     5701 gcttgatgaa atcctcgttc cgaaaagtct tgggcaacgg accaggtcag cggggtggcg
     5761 acgccgggca gggcttcgcc cacattggcg cgagaccaga cggtgttttc atcgccgccg
     5821 ggagggaagc cggaccccac gatgggcctg gcttgcacga ggaagacctc atcgttttgg
     5881 acggcgaatt cgacgtcgaa ggcgttgttt tctacttgtt cgagagcgtc cgcgaggctg
     5941 gagagttcat gcagggcgac ggtagaaagg gcgggttcgc aagcgcgggg caaggggacc
     6001 gaaacatagt cggggccatg gtctccgatc atgagcgacc ggtctttgac ggcaatggtt
     6061 tgatgcacga cggctcgggt atttttacgc aggcgaacga catccggcac gaccgcgccg
     6121 ttgaccaccg gagcaccgag acccaacgtg gcgttgatga gacgttcgtc ggaggcccag
     6181 accgaatcgt cggttcccgc gggagggcgt gtaaacatca cccccgaggc ctccgcgcgg
     6241 accatgactt ggagaacgac gcccattgcg agatcgcgaa tgccgcgaaa ggcaaggtgt
     6301 tggagagagc gggggagaaa agcgctggcc catacttcac ggattgcgtg gatgagtcca
     6361 tcgacgcctc ggacgccgag cacggaggtg gcgaggccag ccatagcggt caattcggag
     6421 tcctcgcagg tggcgctcga ccggacggca agcccccagg gtgcgtccgc gctcacggtt
     6481 tcccaaagaa attcaagttc ttgcacaagg gacggaggca gcggcgcgga tagccatcgt
     6541 tcgcgggctc gcccgatttg ggataacgca tcggcgcgat tcatgctctg gatcagcgcg
     6601 cgagggtgga gatccgacgg gatttgggaa gctatcgtgg tggagaaaac ggtagcggga
     6661 agaatccagc cgcgcggtgt gcgaaagccg tggcgatgaa gccatgcgag ccgcataccc
     6721 ttgcctccca cctcagccgg gtcgatggga gcgttttcga tgggatacag ccaaaccgtt
     6781 tgagtcacgt cgggaccatc gtagccgaaa gccaagattc atcaccatgg agatggtggg
     6841 gagagtgcgg ggaaacgccg cccttaccgt ccgcgcttcg gccgattttc ggcggctccg
     6901 atgggtgttc gctattcatc cccctaagaa acatttgtca ggcttgttgg cttggcattt
     6961 tcgattcttg tggggagaag cgagggccga gggcaagagg gaatcagggt ttgcagaaat
     7021 ttccctgttc cgcggtgtcg agcaagaagt tggcggtttc caacgaggta cgttccgttc
     7081 cctcgagcca gctcagataa cctgacgcat ggcggaattg tagttgtaga aatgccacgg
     7141 aaagacctcg aaacacgagt tgtcgctttt catgctctgc tttgggccca cgaggacacg
     7201 aaccgcacaa gaggagacaa tcgtaatcgt cgatgaattg caaatgaccg aatgcttcga
     7261 gcacgccgag ccaggcggtc gtcttgtcag gtacggactc gaaaaaccga cacgcgttgg
     7321 aggcggaagg ggcacaggaa ttgctcgaag aaccgagggc agctcccaaa aatagcgcgg
     7381 gaaccttgaa cttacccatt tgctcggggg cgagggacgg acgatggctg ctgcccacca
     7441 aggaaccagg atcgtcgtcc accgggctga gggttatcac cgcggatacg cgcggatctt
     7501 ccagggcaag ccatacggcc gccttgcccc ccaccgcatg ccccatggcc gcaatgcgct
     7561 tgggatcgac cacattgcct acattgtctg ggggtttggt cgtaagaacg tcgatcgctc
     7621 ttttgaggtt ctcgaccggt gcgtgatggt ccgggtcgag taaaccaagg tcataatcca
     7681 ccgatgccgt cacatatccg aactgcgcga tatgtttcat ggttttcttg tagctatctt
     7741 tttgtgcctg atgatcgcga gcgaaaacaa cgagaggata tagctcgccc gagggtggat
     7801 cggccgtggg ccggaagccc accatctcct tggaaaccat gcttcccgga gacaacttgc
     7861 ctttccattc tgttacgggg acgtccggcg acaccatggg atcggggacc gtctcgaccg
     7921 gctcatccac gacttctggc gcgatatccg acgcaacttc caaggctacg tccacgatga
     7981 catcgtcgct cacgtccgga tggtcggtgg cttccgccgc ctggtcggcc gtgatatccg
     8041 gtgccacgtc ttgtgttcca ctatctgcct ggacactgga gggttcgagt agcgactccg
     8101 ccgtggagct gccacatccg atcagcgcca acccaaaaac cacactccac gcgatatatc
     8161 tcatcgtctt tattcatgac acactcacga aatagagcaa tagagcatgt acccaaagca
     8221 aggaaagaaa gcaggattgc agcgagaagc cagactgaaa aatttgaaaa ccgctttggt
     8281 tacaaatagt ta
//
LOCUS       DAOSOR010000215         8279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8595_length_8279_cov_4.330982, whole genome shotgun sequence.
ACCESSION   DAOSOR010000215 DAOSOR010000000
VERSION     DAOSOR010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8279
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8595_length_8279_cov_4.330982"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PK710_12625"
     CDS             <1..810
                     /locus_tag="PK710_12625"
                     /inference="COORDINATES: protein motif:HMM:NF022932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT10613.1"
                     /translation="TRDMAEPLVVQTLVQLGFSLNESRAYHALLLENPSTGYEVGARA
                     KIPRSAVYGVLRRLVQAGLARAVAQRPERFAPVPVDHVVALLKKRFDTSAQEFERAVR
                     TLDVTPSIPDAFSVRGYDRVLEEAERMIHGAKSSLIISGWPREIHKLIAEIDKASKRK
                     VFLVVFSHAKLPKMKGSIFSYELEEAALEAFWKHRLTIVADDTRTLIASVENNERDHA
                     VISETVSLAEVATSQIALDITLLSQRTHQDVEKVMVRMLGERVGRLDTLLS"
     gene            914..1516
                     /locus_tag="PK710_12630"
     CDS             914..1516
                     /locus_tag="PK710_12630"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HOT10614.1"
                     /translation="MSLSHYLQGITRSANTIFEGLAVTFSHLLREPVTIQYPDRTTRP
                     VRDMLPERYRGFLEVQMDICSACKRCERACPIDCISIDTQKHPETKKLMVTRFDIDVS
                     KCMFCGLCAEACEEGATGAIRHTREFEGSVGVIDALVFRFVPPGEQFPMYRTPKDKSE
                     IPLGQVGPHAREARERALRDNPALFDSLRQQTTGEGNQPG"
     gene            1578..2099
                     /locus_tag="PK710_12635"
     CDS             1578..2099
                     /locus_tag="PK710_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOT10615.1"
                     /translation="MSDGYLHIFAFYVLAALTLGSAGIVALSRNIIYSTISLLGTFLG
                     VAGLYISLSADFLAAVQILVYVGGTLTLLLFAVMLTAKIEDVRSSNTSRGRWAAFIVI
                     GLLLLVLGRVATTTAWPTDLREPMPSTAKLGHAFLSEYLLPFEIASVLLLGAMIGSVI
                     LARRAVKRESEEE"
     gene            2103..2411
                     /gene="nuoK"
                     /locus_tag="PK710_12640"
     CDS             2103..2411
                     /gene="nuoK"
                     /locus_tag="PK710_12640"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOT10616.1"
                     /translation="MEIGLLHYLTVAAVLFGFGLFCIITRRHAVGILLGVELILNAAA
                     LNFVAFEHYVAGGTTGQVFTVFIICLAASEAAIALAIVLQVFRAHRTIVADQIQDMKH
                     "
     gene            2665..5286
                     /locus_tag="PK710_12645"
     CDS             2665..5286
                     /locus_tag="PK710_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOT10617.1"
                     /translation="MPSYSGPLQTVVESTTLWLVVFFPVLGAILNAAVGWKWQRSAAG
                     QEISKKLHIGSLPVSGVAVGAMVLSFGLAVWHVVQLVQLPESQRNLLCHLWPMVRVGS
                     FDLSFDLALDPLSAVMLLVVTGVGLLIHVYATGYMETEPSYWRFFSYLNLFIFAMLLL
                     VLGDNFFVMFFGWEGVGLCSYLLIGFWYKDYKKASAGTKAFLTNRVGDFGFLTGLFVL
                     LWALGGSWNEQGYQPDIAPRLVPVTVDHDAGHGEAQPPAAETTTKGVQNAYITFTSFP
                     GAKVFINGDTVGPLVAPFVRYELPLLSDRNSFVIVMGGATEDLRLDNVQLVAGKETIL
                     SPVGTVLSFRDLSNQMVIQDKVTGKRPFFERLKGKSLWGIAAITLACLGFFIGATGKS
                     AQIPLYVWLPDAMAGPTPVSALIHAATMVTSGVYMIARLNFLFSMSSTASGVVAIVGA
                     CTALFAATIGFFQYDIKRVLAYSTVSQLGFMFIGVGVGAYWAGIFHVMTHAFFKACLF
                     LTAGSVIHGMHAVEHDADRAQDMRVMGGLKGRMPRTRLAYLISCLAITAAPIPLLAGF
                     WSKDEILWKAFSTANTGFVPGMLIWAMGLTAAIGTSFYMWRSYYLTFEGPFNWTPEKD
                     NEPGKPFAVASKVLSEKMLPKVHESPPVMTTVLLILAALSAVGGAIFGFNTHHLGYHH
                     GEPGFMPPLLEHWLHPVTQHATVSFKDFGSSALIIEHGLMWSSVLLAIGAWALARKRY
                     GANRSRDWETQERALPGWKLLNAKYYVDEIYHATVIRGVLTLRLIMRDFDKHVVDAIV
                     NFTGVMTKFTAKVDGAIDHYLVDGAVNLVAQSCLQIGARLRKIQTGQIQNYAYGVLGG
                     VATLAVIVFLIRYYGAR"
     gene            5331..7574
                     /locus_tag="PK710_12650"
     CDS             5331..7574
                     /locus_tag="PK710_12650"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOT10618.1"
                     /translation="MSKFVSRLYWVTFVSWLVVLVGATALAQTPPGMPSEATTPEGIQ
                     PVPTANPPLPSHVSPRLMPGPRVVEPPVRTILPGQPELRIEGKRGGPLEMVWDGKVFT
                     GTFEVHNVGTGNLMVTRVAVRQGDNGPWLPPGTSAQLEGNQERVLIAPDASHKVMVRW
                     EPGDQAQLKEWYGHVLVETNAADQQPLAMGVHAELPLGGAPWLFNHILSWLVFLPILG
                     MLAIFALYLAGYKKDENLKWGVLGLMGVELAGAIWLFSMFNRNVGRWAGNEGFQFIER
                     GVWIRSLNVEYFVGVDGTSISMVLLTALVSFVAVLASFSVKTQLKGYWAMFLLLVTGM
                     MGVFVALDLFLFYVFWEVMLLPMYFLIGIWGGPRKEYAAIKFFLYTLAGSVFMLLAFI
                     AMYYTSGPAWLVDGSTVAHTFSIPELMRVDFLNLGQPILGLMFVKVVWVVLFIAFAIK
                     VPMFPFHTWLPDAHVEAPTAISVILAGVLLKMGTYGMLRLNFGLLPEATQWAAPAMAV
                     FGVISILYGAFCAMAQEDLKRLVAYSSISHMGFCLLGMGAMTPHGIEAAIVQMFNHGT
                     ITAMLFSLVGVIYDRAHTREIGKFGGMATEMPFYSAFFGFAFMASLGLPGLSGFIGEA
                     LTFVGAFPVYPALTILAASGVIFSAAYHLWAMQRIFLGKFRESWRSSHALEEFGGKFP
                     EINLREIMTLAPMAVVVLILGFWPRPLLDLIDAGVLDLTALVNPPVASQTATYSEAAR
                     QFLALLP"
     gene            7593..>8279
                     /locus_tag="PK710_12655"
     CDS             7593..>8279
                     /locus_tag="PK710_12655"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOT10619.1"
                     /translation="MQTLDNLQSLSFFLPEFALIAGVLLVVFWDLMARGTSRIIGIVS
                     FALAALATSCIISVGYLVNSLPSQKLFYGLLAFDAYANLFRIVFACIGAIIIIFAVPA
                     PETGAGTEKRKGAGEFFALLLVITLGLNLMVMSRNLLMIYLSIELVSVLSFVLAGFKL
                     KDRRSSEGALKYVIFGGMASGVMLYGMSWLYGLAQSLDLAEIAARVALETANEGHIPL
                     AILVGVICMLA"
BASE COUNT         1522 a         2281 c         2365 g         2111 t
ORIGIN      
        1 accagggaca tggctgaacc cttggtcgtc cagacactcg tgcaactcgg ctttagcttg
       61 aacgagtcgc gcgcgtacca tgctttgcta ttggagaacc cgagcacggg ctatgaagtg
      121 ggcgcccgcg ccaagattcc tcgctccgcg gtctacggtg tattgcgacg tttggtgcaa
      181 gcgggattgg ctcgggcggt ggcacagcga cccgaacgtt tcgcaccggt tcccgtcgat
      241 catgtagtcg ccttgttgaa aaaacgtttc gatacctcgg cccaagagtt cgaacgagcc
      301 gtccggactc tcgacgtcac gccttcgatt cccgatgcct ttagcgtgcg aggttacgat
      361 cgcgtgttgg aggaagcgga acggatgatt cacggtgcca agagttcctt gatcatcagt
      421 ggctggcccc gcgaaatcca taagcttatc gcggagatcg ataaggcctc caaacgcaag
      481 gtctttctcg tggtcttttc tcacgccaaa ctccccaaaa tgaaaggctc gatcttcagt
      541 tacgaactgg aggaggctgc gctcgaggcg ttttggaaac atcgattgac catcgtcgcg
      601 gatgacacac gaacgctcat cgcttcggtg gaaaataatg agcgagacca cgcggtcatc
      661 agtgaaaccg tatcgctggc ggaagtcgcc acgagccaga tcgctttgga tatcaccctg
      721 ctgtcccagc gaacccatca agacgtggaa aaggtgatgg ttcgtatgtt gggtgagcgg
      781 gttggacgct tggacacctt gctatcgtga gccaggggca tcggatcgtt atttcccctt
      841 gcagcgctcg acgctatggt gggacgctgt ggctgcgctt gatccatcga gctttcggtc
      901 ccatggaatc atcatgagtc tgtctcatta cctccaagga atcacgcgat ccgccaacac
      961 gattttcgaa gggttggcgg tcacgttttc acatcttttg cgggagccgg tgacgattca
     1021 ataccctgat cgcaccacgc gtccggttcg tgacatgctt cccgaacggt atcggggttt
     1081 tctcgaagtg cagatggata tttgttcggc gtgcaaacgt tgtgaacgag cttgtcccat
     1141 cgactgcatc tcgatcgata cacaaaaaca tcccgaaacg aagaagctga tggtgactcg
     1201 cttcgacatc gatgtgtcca aatgcatgtt ctgtgggctg tgtgcggaag cttgtgaaga
     1261 aggcgcgacg ggagcgattc gccacacgcg cgaattcgaa ggaagcgttg gcgtcatcga
     1321 tgccctggtg tttcgattcg tgcctccggg agagcagttc ccgatgtatc gaactcccaa
     1381 agacaagtcc gagatccctc ttgggcaggt tggcccgcat gcaagagagg cgcgggaacg
     1441 tgcgcttcga gacaatcccg cactattcga ttccttgcga caacagacca cgggtgaggg
     1501 aaatcaaccg ggctgaggct catcggcagc agcctcttgc ggtgttgcgg gcgacgatct
     1561 agcctgccac cgctcccatg tccgacggct atctccacat cttcgcgttt tatgttcttg
     1621 cggcgcttac gttgggttcc gcgggaatcg tggcgctttc tcgtaacatc atttactcca
     1681 cgatctcgct gctcggaaca ttcctcggcg tcgctgggct gtatatctcg ctgtccgcag
     1741 acttcctcgc cgcggtgcaa atcctcgtgt atgtcggcgg caccctcacg ttgctcttgt
     1801 tcgccgtcat gctgaccgcc aaaatcgaag atgtgcgatc gtcgaatacg tcgcgaggac
     1861 gatgggcagc gtttatcgtc atcggtctgt tgctgctcgt gctcggacga gtggctacca
     1921 cgaccgcctg gccgacggat cttcgtgaac cgatgccttc gacggccaag ctcggtcatg
     1981 cctttctcag cgagtacctg cttcctttcg agattgcctc ggtgttgctg ctcggtgcca
     2041 tgatcggttc cgtcatcctt gcccgtcgtg cggtcaagcg tgaatccgag gaggagtgaa
     2101 ggatggaaat cggactccta cattatctca ctgttgccgc agtcctcttc ggttttggcc
     2161 ttttctgcat catcacgcga aggcacgccg taggcatcct gctgggcgtg gaactgatcc
     2221 tcaatgccgc ggcgctgaac ttcgtcgctt tcgagcatta tgtggcggga ggcacgacgg
     2281 gccaggtctt tacggtgttc atcatctgct tggccgctag tgaggcggcg atcgctttgg
     2341 ccatcgtatt gcaagttttc cgtgcccacc gaaccattgt cgccgaccag atccaggata
     2401 tgaagcactg ataatctaga attccagcga ttactgtcga cgtaacgagg ggtgaggccc
     2461 ttgccatagg gtcatattcg tgctcatttc acgttattcc tcccaacccc ctccatctgc
     2521 tttgaatcct ctcggtttgt tgcgtctttg ctcgtccgtg atgcttgacc cctcactcca
     2581 ccgtccgcta acgtgccccc gccttcgcgc ccagtgcgat gcggctgtcg cacgtcaacg
     2641 gcttgaccgc gaggtttcac gaagatgcct tcctactcag gccctctgca aacggtcgtc
     2701 gaatcgacaa cgctctggct agtggtgttt tttccggtcc tcggcgccat cctgaatgct
     2761 gctgttggct ggaaatggca acgatccgcc gcgggccagg agatttccaa aaagctgcat
     2821 atcggtagcc ttccagtctc tggtgttgcc gttggcgcga tggttctgtc tttcggcctc
     2881 gcggtgtggc acgtcgtgca actcgttcaa ttgcccgagt cgcagcgaaa tctgctgtgt
     2941 catctatggc cgatggttcg agtgggttcc tttgacctgt cgttcgatct ggccctcgat
     3001 cctctttccg ccgtcatgct tcttgtcgtt accggcgtag gcttgctgat ccacgtatat
     3061 gccactggat atatggaaac ggagccatcg tattggcgtt tcttcagcta cttgaacctg
     3121 ttcattttcg cgatgttgtt gcttgtgctc ggtgacaatt tctttgtgat gttctttggt
     3181 tgggaagggg tggggttgtg cagttatctg ctcattggct tctggtacaa ggattacaaa
     3241 aaggcttccg ctggcacgaa agcgtttctg accaaccgtg tgggtgactt tggtttcctc
     3301 acggggttgt tcgttttgct ttgggcactt ggtgggtcgt ggaacgagca gggctatcaa
     3361 ccggacatcg cgccgcgttt ggttcctgtc acggtcgatc atgatgcagg tcatggtgag
     3421 gcgcagcccc ccgcggctga aaccacgacc aaaggcgtgc aaaacgccta tattacgttc
     3481 acatcttttc caggggccaa ggtcttcatc aatggtgaca cggtgggccc gttggtggcc
     3541 cccttcgttc gttatgagtt gccgttgcta tccgatcgca actcgttcgt catcgtcatg
     3601 ggtggtgcga cggaagatct tcgtctcgac aacgtgcagc tcgtcgccgg aaaagagacg
     3661 atcctatctc ctgtgggcac ggtcctatcg tttcgtgatc tatccaatca gatggtgatt
     3721 caggacaagg tgacgggcaa acgtccgttt tttgaaagac tcaaaggaaa atccctgtgg
     3781 ggaattgcgg cgataaccct ggcgtgtctt gggtttttca tcggcgcaac cggaaaaagt
     3841 gcgcagattc ccctgtatgt ttggctcccg gatgcgatgg ctggtcccac gcccgtgtcc
     3901 gcgctcattc atgcggccac gatggtgacc tcaggcgtgt atatgatcgc acgactgaat
     3961 tttctgtttt cgatgtcttc cacggccagt ggagtggtgg ccatcgtggg ggcatgcacc
     4021 gcccttttcg cggcaaccat tggcttcttc cagtatgaca tcaagcgagt gctggcctac
     4081 agcaccgtaa gtcagcttgg gttcatgttc atcggggtag gggtcggtgc ttattgggcc
     4141 ggcatcttcc acgtgatgac gcatgcgttt ttcaaagcct gcctctttct caccgccgga
     4201 tcggtcattc atgggatgca tgctgtcgag catgatgccg atcgtgcgca agacatgcgt
     4261 gtgatgggag ggctcaaagg caggatgcca cgcacgcgat tggcctattt gatttcctgt
     4321 ctcgcgatca cggcggcgcc catcccactt ttggccgggt tctggtcgaa agatgagatc
     4381 ctttggaaag cattttccac agctaatact ggatttgtac cggggatgct catctgggcg
     4441 atggggttga ccgcggccat tggcacttct ttttatatgt ggcgcagcta ctacttgacc
     4501 ttcgagggcc ccttcaactg gacaccggag aaggataacg agccaggcaa gcccttcgcc
     4561 gttgcctcca aggtactaag cgaaaagatg ctgccgaaag tgcatgaatc accgcctgtg
     4621 atgacgacgg tactgttgat tctcgcggct ctttccgcgg ttggcggtgc catcttcggc
     4681 ttcaacacac atcacttggg ctatcaccac ggtgagccag ggttcatgcc gccgctattg
     4741 gagcattggc tccatcccgt gacacaacat gcaacggtat ctttcaagga ttttggttcg
     4801 agtgccttga tcatcgagca tgggttgatg tggagttccg tgctgttggc gattggagct
     4861 tgggccttgg ctcgtaaacg gtatggcgcg aatcgatcac gggattggga aacgcaagag
     4921 cgagccctgc cgggctggaa attgctcaat gccaagtatt acgtcgacga aatctaccac
     4981 gctaccgtga tccgtggggt ccttaccctg cgcctgatca tgcgggactt cgacaagcat
     5041 gtggtcgatg cgatcgtcaa cttcacgggc gtgatgacca agttcactgc caaggtcgat
     5101 ggcgcgatcg accactacct cgtggatggc gcggtgaatt tggtggcgca gtcttgtctg
     5161 caaataggcg cgagactgcg aaagattcag accggtcaga ttcaaaacta cgcgtatggt
     5221 gtgttgggtg gtgtcgccac cttggcggtc atcgtgtttt tgattcgtta ctacggggca
     5281 cgctgatgaa agcgtcacct tggggttctc agatagggag ccacttcgcg atgagcaagt
     5341 tcgtatcgag attgtactgg gtcaccttcg tttcctggct tgtggtgttg gtgggggcaa
     5401 cagccctcgc acaaacacca cctggtatgc cgtcggaagc tacgactccg gaggggatac
     5461 aaccagtccc gaccgcgaat ccaccgcttc cttcccacgt ctctccgcgc ctcatgccgg
     5521 ggcctcgcgt ggtggagccg cctgttcgaa ccatacttcc cggccagccg gaacttcgca
     5581 tcgaaggcaa acgaggcgga ccgttggaaa tggtctggga cggaaaggtg ttcacaggca
     5641 ccttcgaagt ccacaatgtc ggtacaggca atctcatggt gactcgtgtg gccgtgcgtc
     5701 aaggagacaa tggcccatgg ctgccccccg gaacgtccgc gcaactcgaa ggcaaccaag
     5761 aacgcgtgct gatcgctccc gatgcatcgc ataaggtcat ggtgcgctgg gagcccggtg
     5821 atcaggcaca gctcaaagaa tggtatgggc atgtgttggt ggaaaccaat gctgccgatc
     5881 agcagccgct agccatgggc gttcatgcgg aacttccttt gggaggcgcg ccttggcttt
     5941 tcaatcacat cctgtcttgg ctggtgtttt tgcccatcct tggcatgctg gccatttttg
     6001 ccctgtatct cgcgggatac aagaaagacg aaaacctcaa atggggtgtg ttgggactca
     6061 tgggagtcga gttggctggc gccatttggc tgttttcgat gttcaatcgc aacgtggggc
     6121 gctgggcagg taacgaagga tttcaattca tcgaacgcgg agtgtggatc cgttctttga
     6181 acgtcgaata cttcgtgggt gtcgatggca cttccatttc gatggtgctt ctgacggctc
     6241 ttgtgtcgtt cgtagccgtg ttggcgtcgt tcagtgtgaa gacccagctc aaaggctatt
     6301 gggccatgtt cttgctgctg gtcacgggaa tgatgggcgt gttcgtcgct ctggatctgt
     6361 ttttgtttta cgtattctgg gaggtgatgc tgctgccgat gtacttcttg atcggcattt
     6421 ggggtggacc tcgcaaagaa tacgcggcca tcaagttctt cctgtacacg ctggcaggct
     6481 ccgtcttcat gctgctggcc ttcatcgcaa tgtattacac cagcggtccc gcatggctcg
     6541 tcgacggcag tacggttgcc catacgtttt cgattcctga gttgatgcgt gtggacttct
     6601 tgaacctggg ccagccgatc cttggattga tgttcgtcaa ggtcgtgtgg gtggtgctgt
     6661 tcatcgcttt tgccatcaaa gtcccgatgt tcccgttcca cacgtggttg cccgacgcac
     6721 acgtggaagc gccgacggcc atcagtgtga tcctggcggg agttttgctg aagatgggca
     6781 cctacggcat gcttcggctc aacttcgggc ttttgcccga ggccacgcag tgggccgccc
     6841 cggcgatggc tgtttttggt gtcatcagca ttttgtatgg cgctttctgc gcgatggcgc
     6901 aggaagatct caaacgattg gtggcttatt cgtcgatcag tcacatgggg ttctgtctgc
     6961 ttgggatggg ggcgatgacg cctcatggca tcgaagcggc tatcgtgcag atgttcaacc
     7021 acggtacgat cacggccatg ctcttttcgc tggtgggtgt gatttatgac cgtgcccaca
     7081 cgcgagagat aggcaagttc ggcggtatgg ccacggaaat gccgttttac tcggcatttt
     7141 ttggctttgc ctttatggcc tccctcggtc ttcccggctt gtcgggcttc atcggtgaag
     7201 ctttgacgtt cgttggcgct ttccccgtct atccggcttt gaccatcctc gccgcctcgg
     7261 gtgtcatctt cagtgccgcc tatcacctct gggctatgca gcgcatattc ctcggaaaat
     7321 tccgtgaaag ctggcgcagc agccacgcgc tcgaagaatt cggaggaaag ttcccggaaa
     7381 tcaacctgcg cgagatcatg acccttgctc ccatggccgt cgttgtgctg atccttggct
     7441 tctggcctcg acccctgctc gatctcatcg acgcgggagt tctcgacctg acagccctgg
     7501 tcaatccgcc tgtcgccagc cagactgcaa cctattccga agccgcccgg caattcctgg
     7561 ctttgcttcc ctgacgtcgc gaggagatgt cgatgcaaac cctcgacaac ctgcagagcc
     7621 tgagcttctt cctgccggaa ttcgccctca tcgctggcgt gttgctcgtt gtgttctggg
     7681 acctcatggc tcgaggcaca tcgcgcatca tcggcatcgt gtcgtttgcc ttggccgcct
     7741 tggccacgtc ttgcatcatc tccgtgggct atttggtcaa ttccctgccc tcgcaaaagc
     7801 tgttttatgg cttgcttgcc tttgatgcct acgcgaactt gtttcggatc gtattcgcct
     7861 gcatcggcgc catcatcatc attttcgcgg taccagcgcc cgaaaccgga gcgggaaccg
     7921 aaaaacgaaa gggcgccggt gagttctttg ccctgcttct ggtcatcacc cttggactca
     7981 acctcatggt catgagccga aacttgctca tgatttatct gtccatcgaa ctcgtaagcg
     8041 ttctgtcgtt cgtcctggct ggcttcaaac tcaaagaccg acgctcctcc gaaggcgccc
     8101 tcaaatacgt catcttcggt ggaatggctt ccggcgtcat gctctatgga atgagttggc
     8161 tctacggact cgcgcaatcg ctcgacctgg cggaaatcgc ggcccgtgtt gctttggaga
     8221 cggcaaacga aggccatatt cccctcgcca ttctcgtagg cgtgatctgc atgctcgcg
//
LOCUS       DAOSOR010000216         8274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8607_length_8274_cov_4.721256, whole genome shotgun sequence.
ACCESSION   DAOSOR010000216 DAOSOR010000000
VERSION     DAOSOR010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8274
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8607_length_8274_cov_4.721256"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1370
                     /locus_tag="PK710_12660"
     CDS             <1..1370
                     /locus_tag="PK710_12660"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT10620.1"
                     /translation="FHVSLPQARRDIAYYLRLQEEGAEGVRLLDVTSPETGKFDYKVY
                     TRVVRNTTNRSPSEFKEMQREEIIAERMRQLIASRASITDTEAFDVYQREKSSAKLEV
                     VRLDRDYFAAKYIDTSEKAVESWAKEHTKDIDDSWNARKSSFPAGCVKARHILVKVQS
                     TTVPQGHPREEAQSLIEKARSRIAGGELFPVVASSMSEDSASADKGGDLGCIVKGKYP
                     QAFDDAVFSQKKPGLIDTVIETNYGFHLVDIEMIVSENAQTAEAQGRMQIAKELMLAV
                     ETEKLISETAKHIQESAQKGTALQQAIDTVLAKLDEERGIKKKAAKKEEEETPTEPGR
                     PFIETTASFSQLDGNPIDGVARGQNVVEMAFRLSKPGEVGSDLVRLNNGYAVIQLKER
                     TSASREDFDKERHEYAARLLYVKQQDLLVSYIEQLRKAAEKEITIAEKWVQEPKRRSG
                     SDDDE"
     gene            1385..2680
                     /locus_tag="PK710_12665"
     CDS             1385..2680
                     /locus_tag="PK710_12665"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOT10621.1"
                     /translation="MQLSNLKVHRTTLDRGLRLVCVEQPLLHGICVSLHVPAGPRFEQ
                     TSNNGCSHMLEHMLFRGTPEHPSAHALAVAIERLGGTLYGATYTDHGHMSVTLPSETF
                     ERGLELFAHVVTQPVFADMDVERGIIREEILEGLDEDGQCIEPDNLTRAVMFPSHPLG
                     MPITGTLATLDTLGETVLRQHHARLYTANHAVLCIAGPIEAKRCEQLVAAAFQRMPAG
                     QPLALQPPTAPNGPRFLYTESQGSQTDLRLAFRAPGRNDPSEPAAELLVRLLDDGMST
                     RLYARICDEMGLCYDVSAEYEPFEDTGVLDLAATVRHERAPLVMRELCGIVAQLADQG
                     PSEEEMRVAKNRAHWGLESLLDHAEGLADYHGLSELHGLERTLIERRDKVERVRRDEV
                     VEVARRVFSPGQGAAVAVGMLKKTQRKEIEMALASLRNG"
     gene            2839..3282
                     /locus_tag="PK710_12670"
     CDS             2839..3282
                     /locus_tag="PK710_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10622.1"
                     /translation="MRDRVYLLTFGLMMIVFTGLGGCSALGACDRSDDGNPADPYYDG
                     TAEGGLYMSSPWTGPLLPFPGGKRYRLYHHLGCTPRLLECWASFNSSGIENGSIAPPA
                     GNMCVVQEINDEFIQIKNDTCSDMYVLVTAEASCEKTEPTSDGGT"
     gene            3279..3593
                     /locus_tag="PK710_12675"
     CDS             3279..3593
                     /locus_tag="PK710_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10623.1"
                     /translation="MKRAWVLLISIVLACQSDPIESPEPAAALGRCCAEARSRVETVK
                     DRETAQFQKWCNACRKGDSKASCLSAAKNVLHATQNAFGEYGMPVSCSAMMQSLQESG
                     IH"
     gene            3802..4092
                     /gene="groES"
                     /locus_tag="PK710_12680"
     CDS             3802..4092
                     /gene="groES"
                     /locus_tag="PK710_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOT10624.1"
                     /translation="MNIRPLHDRIIVKRVQEEEKTKGGIIIPDTAKEKPIEGEIVAVG
                     NGKIMEDGSVRKLEVKAGDRILFGKYSGTEVKIDGEERLIMREDDVLCVVER"
     gene            4132..5745
                     /gene="groL"
                     /locus_tag="PK710_12685"
     CDS             4132..5745
                     /gene="groL"
                     /locus_tag="PK710_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456581.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOT10625.1"
                     /translation="MSAKEIVYTEAARGLILNGVNALADAVKVTLGPKGRNVVIEKSF
                     GSPTITKDGVTVAKEIELENKFENMGAQMVREVASKTSDVAGDGTTTATVLAQAIFRE
                     GTKLVAAGHNPMEIKRGIDKAVSTLVESLKSTAKPTKDPKEIAQVGTVSANGDETIGK
                     LLADAMEKVGKEGVITVEENKSAETTLDVVEGMQFDRGYLSPYFVTDPERMEVSLEDA
                     YLLISEKKVTSMKDLLPVLEAIAKQQKPLLIIAEDVEGEALATLVVNKLRGTLSCVAV
                     KAPGFGDRRKEMLKDISVLTGGQVIAEELGLKLENVTISDLGKAKRIQIDKDNTTIVG
                     GAGNKDKIKGRIAEIRTQIENTTSDYDREKLQERLAKLVGGVAVVKVGAATEMEMKEK
                     KARVEDALHATRAAVEEGIVAGGGVAFLRAQASLDKLQVSDEQRVGVQIIRRAIEEPT
                     RQIVENAGEEGSIIVKAIKDGKGNFGYNAQTGEFGDLMQMGVIDPLKVVRTALENAAS
                     VSGLLLTTEALVAEKPKDDKEGAPSMGDY"
     gene            6020..6463
                     /locus_tag="PK710_12690"
     CDS             6020..6463
                     /locus_tag="PK710_12690"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10626.1"
                     /translation="MIQQAVMQVLYPIFAPTFSDNSHGFRPGKGTHGSLAIAAGRRAA
                     RPGVRFASRTRIGLPRATAASTRHRARRAVETAWFRAGRARPTAVTPQLCSHSADNRA
                     GPVRTRVARGGRTKAARSNDEASGARGSCNIARGSPRSGPSTKNI"
     gene            6916..7434
                     /locus_tag="PK710_12695"
     CDS             6916..7434
                     /locus_tag="PK710_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10627.1"
                     /translation="MGDAVKDAKLSDSVSGQIPIWLDVGYRIDESLFVGAYGQYGITM
                     VKDCPDGVDCSASDIRFGVQGHYHFMPGESFDVWAGLGIGYEMLSSSAKAGGHEASST
                     LSGFEFANLQVGGDFQVAEGMGVGPFVSFSLGQYGKVKAEVPGAGTSDGDIEDKGMHQ
                     WLLLGVRGSFQL"
     gene            7991..>8274
                     /locus_tag="PK710_12700"
     CDS             7991..>8274
                     /locus_tag="PK710_12700"
                     /inference="COORDINATES: protein motif:HMM:NF019984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HOT10628.1"
                     /translation="MAELPGVGGLNFLHRLKFNRTKSAVARPEERTFLGNTITRCDDQ
                     PQLEVAAKSVQRLQAKLKPLFRQGREMKLANTNGRNNRIIRGWTAYFRLS"
BASE COUNT         1900 a         2174 c         2511 g         1689 t
ORIGIN      
        1 gttttcatgt ttcgctgccc caggcgcgaa gggacatcgc ctattatctt cgtctgcaag
       61 aggagggggc cgaaggggtg cgtttgctcg acgtgaccag tccggagacg ggaaagttcg
      121 attacaaggt gtatacccgg gttgttcgca acaccaccaa ccgtagtcca tcggagttca
      181 aggagatgca gcgtgaggaa atcatcgccg agcgcatgcg tcaactcatc gcgtcacggg
      241 ccagcattac ggatacggaa gctttcgacg tatatcagcg cgaaaagagc agtgccaagc
      301 tcgaagtcgt gcgactcgac cgggattatt tcgccgcgaa gtatatcgat acatcggaaa
      361 aggccgtgga gagctgggcc aaggaacaca ccaaggacat tgatgattcc tggaacgctc
      421 gtaaatcatc gtttccggcg ggctgtgtca aagcgcgtca tattttggtg aaagttcagt
      481 ccaccacggt tcctcaaggt catcctcggg aagaagcgca atccctcatc gaaaaggcac
      541 gttcgcggat tgccgggggt gaattgttcc ccgtggtggc ttcgtcgatg agcgaggatt
      601 ccgccagcgc ggacaagggc ggcgaccttg gctgtatcgt caagggcaag tatccgcaag
      661 ccttcgatga tgccgttttt tctcagaaaa aacctggtct tatcgatacc gtgatcgaga
      721 cgaactacgg ttttcatctg gtcgacatcg aaatgatcgt cagtgaaaat gcgcagacgg
      781 cggaggcgca ggggcgcatg caaatcgcca aggagttgat gctggcggtt gaaaccgaaa
      841 agctcatttc cgagacggcc aagcacattc aagaatcggc gcagaagggg acggcgcttc
      901 agcaagccat cgacacggtg cttgcgaagc tcgacgaaga gcgtgggatc aagaaaaagg
      961 cggcgaaaaa agaggaggaa gagacgccta cggaaccggg tcgccccttc atcgagacca
     1021 cggcctcttt ctcacaattg gatggcaacc ccatcgacgg tgtggcgcgt ggacaaaacg
     1081 tggtcgaaat ggcttttcgc ctgtccaaac cgggggaggt cggctccgat ctcgtacgcc
     1141 tcaacaacgg ttatgccgtc atccaactca aagagcgcac ttccgccagc cgagaggatt
     1201 tcgacaaaga gcgccatgaa tacgcggcgc gcttgctcta tgtcaaacag caagacttgc
     1261 tcgtcagcta catcgagcaa ctgcgaaaag cggctgaaaa agaaatcacc atcgccgaaa
     1321 aatgggtgca ggaacccaaa cggcgtagtg gttcggatga cgacgagtag gtgctgtcga
     1381 gcccatgcaa ttgtcgaatc tgaaggtcca tcgcacgacc ttggatcgtg gtttgcggct
     1441 ggtttgtgtg gaacaaccgt tgctgcatgg aatctgtgta tccctgcatg taccagccgg
     1501 tcctcgtttc gagcaaacga gcaacaatgg ctgctctcat atgctcgaac acatgctgtt
     1561 tcgtggcacg ccagagcatc ctagtgccca cgcgcttgct gtcgccatcg aaaggctcgg
     1621 tgggactttg tacggggcta cgtacaccga ccacggacac atgagtgtca cgctcccttc
     1681 cgaaaccttc gaacgaggtt tggaattatt cgctcacgtc gttacacaac cggtgtttgc
     1741 ggacatggac gtggagcggg gcatcatccg cgaggagatc ctcgaaggac tcgatgaaga
     1801 tgggcagtgc atcgaaccgg acaatttgac acgagctgtg atgttcccat cgcatccgct
     1861 cggcatgccg atcaccggaa cgcttgccac gttggacacg cttggtgaaa ccgtgcttcg
     1921 ccagcaccac gctcggcttt acacggcgaa tcatgccgtg ctttgcatcg cgggtcccat
     1981 cgaggcgaag cgatgtgagc agcttgtcgc cgcggcattt caacggatgc ccgcggggca
     2041 acctcttgca ttacagcctc cgaccgctcc gaatggaccg cgttttctgt ataccgaaag
     2101 ccaagggagc caaaccgatc ttcggttggc gtttcgtgcg ccggggcgca acgacccttc
     2161 cgaaccggcc gccgaattgc tggttcggtt gctcgatgat gggatgtcca cacggcttta
     2221 tgcccgtatc tgcgatgaaa tggggttgtg ctacgacgtt tcggcggaat acgaaccgtt
     2281 tgaggatacg ggagttctcg acttggccgc aacggtacgt catgaacgcg caccgctggt
     2341 gatgcgggaa ttgtgcggta tagtcgcgca gttagccgat caaggaccca gcgaagaaga
     2401 gatgcgcgtg gcaaagaacc gcgcccattg gggattggaa tcgttgctcg accatgccga
     2461 agggctagcg gattaccatg ggctatcaga gcttcatggt ttggaacgaa cgctcatcga
     2521 acgacgggac aaggtcgaga gagtgaggcg tgatgaagtg gtggaggtag cgcgtcgcgt
     2581 gtttagccct gggcaagggg ctgcggtagc ggtgggcatg ctcaaaaaaa cgcagcgcaa
     2641 ggagattgag atggccttgg cttcgttgcg gaatggatga gtctggtgaa ccacgcctgg
     2701 actccagggc atggaccgtt atgatgcgcg ttgtgatgcg cggttgcctt tttggttgcc
     2761 tttccacccg aatcaaaacg gtacgccagc cgtttttcgt cgatagcggc gctgatgctg
     2821 gagaaatagg agcggcccat gcgtgaccgg gtctatctgc tgacgtttgg cttgatgatg
     2881 atcgtgttca caggtcttgg aggttgttcc gcgcttggcg cctgtgatcg gtcggacgat
     2941 gggaatccag cggatcctta ttacgatggg accgcagagg gcgggttgta catgtcgtcg
     3001 ccgtggacag gtccgttatt gccgtttcct ggcggaaagc ggtatcggct ttatcatcat
     3061 ttgggttgca cccctcgctt gctggaatgc tgggcatcgt tcaattcgag cggtatcgaa
     3121 aacggttcca tcgcacctcc cgccggcaat atgtgcgtcg tgcaagagat caacgatgag
     3181 ttcatccaga tcaaaaacga cacctgctcg gatatgtacg tgctggtaac cgcggaagca
     3241 tcgtgcgaaa aaacggagcc gacatcggat ggtggaacat gaaacgagcg tgggtattgc
     3301 tgatttcgat cgtcctggcc tgtcaatcgg accccatcga gtcaccggaa ccggcagcgg
     3361 cgttaggccg atgctgcgcg gaagcgaggt cgcgggtgga gacggtgaaa gacagggaaa
     3421 cggcgcagtt tcagaagtgg tgcaacgcct gtcgaaaagg ggattccaag gcttcctgtt
     3481 tatcggcggc gaaaaatgtg ttgcatgcga cccaaaacgc ttttggggaa tacggcatgc
     3541 ccgtatcgtg ctcggccatg atgcagagtc tccaagaatc agggattcac tgaaacgatt
     3601 catcccctgc atatacgttc aggaaaatcg ggctaaaaat cgtagcgaac cgccccttga
     3661 cacccgcaaa tccgtcacta ccttccgctc cgccttcgct agcactcatc tgaatcgagt
     3721 gctaacaacg ggcgactgga aggagcgcac ggccctttcc gactcgacac tatctcagcc
     3781 aggcacggca cggaggaaag catgaacatt cgaccgctgc atgatcgcat catcgtcaag
     3841 cgtgttcagg aagaagagaa aaccaagggt gggatcatca tcccggatac cgccaaggaa
     3901 aagcccatcg agggtgagat tgtcgccgtc ggcaacggca agatcatgga agacggcagt
     3961 gttcgcaagc tcgaggtcaa ggctggtgac cgaatccttt ttgggaagta ttcgggcacg
     4021 gaggtcaaga tcgacggtga ggagcggctc attatgcgtg aagacgacgt gctatgcgtg
     4081 gtcgagcgct gaggcgaatg gttctatctc tgcaaagcac ggagtaaagc gatgagtgca
     4141 aaggaaattg tctacacgga agcagctcgt ggcctcattt tgaacggcgt caacgcgctt
     4201 gcggacgcgg tcaaggtcac cctcggcccc aagggtcgca atgtcgtgat tgaaaagagc
     4261 tttggttctc ccacgatcac caaggacggc gtcacggtgg cgaaggaaat cgagcttgaa
     4321 aacaagttcg aaaacatggg agcgcaaatg gttcgggagg tcgcttcgaa gacctcggac
     4381 gtagctggcg acggcaccac gacggccacg gtgctcgctc aggcgatttt ccgcgagggc
     4441 acgaagctcg tcgcggcggg ccacaacccg atggaaatca agcgtgggat cgacaaagcg
     4501 gtcagcacgt tggtcgagtc gctcaagtcc acggcgaaac ccaccaagga tccgaaggag
     4561 atcgcgcagg tcggtacggt cagcgccaac ggcgacgaga ccattggcaa gctgcttgcc
     4621 gacgccatgg aaaaggttgg caaagaaggc gtcatcacgg tcgaggagaa taagagtgcg
     4681 gaaaccacgc tcgatgtggt cgaaggcatg caattcgacc gcggttattt gagcccttac
     4741 ttcgtcacgg atcccgagcg catggaagtt tccctcgagg acgcctactt gctgatttcc
     4801 gaaaagaagg tcaccagcat gaaggacctg cttccggtgc tcgaggcgat tgccaagcag
     4861 caaaagcctt tgctcatcat cgcggaagac gtcgagggcg aggccctggc taccctcgtg
     4921 gtcaacaagc tgcgcggcac tctttcctgc gtcgcggtga aagctcccgg cttcggtgac
     4981 cgacgcaagg aaatgctcaa ggatatttcc gtacttacgg gtggtcaggt gatcgccgaa
     5041 gaactcggcc tcaagctcga aaacgtcacc atcagcgacc tcggcaaagc caagagaatc
     5101 cagatcgaca aggataacac caccatcgtc ggtggcgccg gcaacaagga caagatcaag
     5161 ggacggatcg ccgagattcg tacgcagatc gaaaatacga cgagcgatta cgatcgagag
     5221 aagctgcaag agcgtctggc gaagctcgtg ggcggcgtgg ccgtggtcaa ggttggcgcg
     5281 gctaccgaaa tggaaatgaa agaaaagaaa gcccgcgtgg aagatgccct gcacgcgacg
     5341 cgtgccgcgg tcgaagaagg gattgtcgcc ggtggcggcg tggctttcct gcgagcccag
     5401 gcttccctcg acaaactgca ggtctccgat gagcagcggg ttggcgtcca aatcattcga
     5461 cgcgccatcg aagaacccac ccgccagatc gtcgaaaacg ctggcgaaga aggctctatc
     5521 atcgtcaaag ccatcaagga tggcaaaggc aacttcggat acaacgcgca gacgggcgaa
     5581 ttcggtgacc tgatgcaaat gggcgtcatc gacccgctca aggtcgtgcg cacggctctc
     5641 gagaacgcgg ctagcgtttc cgggttgctg ttgaccactg aggcgctcgt ggcggaaaag
     5701 cccaaggatg ataaagaggg cgctccgtcg atgggcgatt actaatccga atcacctgag
     5761 caatgactga caaggggctc gcgcatagcg agcccttttt ttgttgggtt caatcgggcg
     5821 gcccatagcc tgtggagata tgtttatcaa tattatccga tagttacaaa tagtgccgcc
     5881 ccgtgatcca gccgttcttt gctcttgttt cgggtgttcc ggcatcggta cttatgttcc
     5941 gcaagctatc cgcaaggaag aaatacccaa agcagacggt ggaaccagga cgttgggtat
     6001 tccgacggtg gtggatcgga tgatccaaca ggcggtgatg caggtactgt atcccatttt
     6061 cgccccgacc ttttcggaca atagccacgg ctttcgaccg ggaaagggga cacacgggtc
     6121 gcttgccatc gctgcagggc gtcgtgctgc tcggcccggc gtccggtttg ccagtcgaac
     6181 ccgtatcggg ctgccacgtg cgacagcggc atcgacgagg catcgagcgc gacgtgctgt
     6241 tgaaaccgca tggtttcgtg ctggtcgtgc tcggcccacg gcagtaactc cacagctttg
     6301 cagccacagc gccgacaatc gagccggtcc ggtgcgtact cgagttgcac ggggcggccg
     6361 caccaaggca gctcggtcca acgacgaagc ctcaggtgct cgtggctctt gcaacatcgc
     6421 gcgaggcagt ccgcgcagcg gcccatccac aaaaaatatc tgagtagggc ggcgacggtg
     6481 gtgacgtgtt gggaacgcac aggtgtgtga agctaccgaa taggttggga gtcacgggga
     6541 tccatagcgt gccaaccaat tgaaatcatg agggttgcag accaattggc gcgaccgttt
     6601 tgccagcgcc ggtgatgact ctgctggaac aatgccaagt cacaccgaaa attttgtagc
     6661 tgctctgaac aacgaataga atcttgacga ttgcagaaat agccctgata tcggggatgt
     6721 cgttcggagg gaattttctc ctcgcaaccc cgtagatagg ggtggctcac aacacaggtt
     6781 cattctgttt catagaaagg cacaattatg agaaagctcg ttattgcttc tgtaactttt
     6841 tcggttctgg cacttgctgg gaccgcttcc gccgaaggtt tcgaattggg agcccgtctt
     6901 ggttacggtt tgcccatggg agatgccgta aaagacgcca agctcagcga cagtgtttct
     6961 ggccagatcc ccatctggct cgatgttgga tatcgtatcg atgaaagcct tttcgttggc
     7021 gcttatggcc aatacggcat cacgatggtg aaagactgcc cggatggggt agattgctcc
     7081 gcgagcgaca ttcgcttcgg tgtacaaggc cactatcatt ttatgcctgg cgagagcttt
     7141 gatgtgtggg ctggtctagg aattggctac gaaatgctca gctcgtcagc gaaggctggt
     7201 ggacatgaag catcgagcac cttgtcgggg ttcgagttcg ccaatctcca agtcggtgga
     7261 gatttccaag ttgccgaagg catgggtgtg ggccccttcg tgagcttctc tctcggccag
     7321 tacggcaaag tcaaggctga agtgcccggg gctggtactt ccgatggtga cattgaagac
     7381 aagggcatgc accagtggct gttacttggt gttcgcgggt ccttccagct ctgagcccaa
     7441 acgtctctgc taggcttcgc ctgtgggcga gcggttgacg aattgctcac cccttcccgc
     7501 ttgcatcctc gcgacaatac ttatttttct cgccagccgg cttttgggaa gccaagtcct
     7561 gacaggaatc atcacgggcc gttcggggcg caggcatgat gaactgaacc tgcgctcgca
     7621 agtatggata cgctgccacc ccttttatca acggtgtctt caccgttttt tccggaagga
     7681 aggtatcgaa aatggggtcg agacggatgg aggaaggggg gagtcatggg ggcattagta
     7741 aaaattgtgt aaattcaata ggttaaagat cagcaggttg ccagactccg gggggcatgg
     7801 ttggcaggga gcgaacggca agcagcgcgc ctcgatcgag cagctcgcgg ctggtaactc
     7861 gatggatcag tttcaaccgc tccccaattc tcaacggcgg tatggtatgg tcacccacga
     7921 cgtcgccgcc gcgaagcgat gagacaccca gttcatctcg aggctgaatt ccaagcattc
     7981 catgtcaccc gtggcggagt tgcctggggt gggaggcctg aacttcttgc accgactgaa
     8041 gttcaaccgg accaagagcg cggtggctag gccggaagaa cgcacgttcc tcggcaacac
     8101 catcacccgg tgcgacgatc agccgcagct cgaggtcgca gccaagtcgg ttcagcggct
     8161 gcaagccaag ctcaagccac tgtttcggca aggccgcgag atgaaactcg cgaacaccaa
     8221 cgggcggaac aaccgcatca ttcgtggttg gaccgcttac ttccgactca gttg
//
LOCUS       DAOSOR010000217         8247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8636_length_8247_cov_3.904419, whole genome shotgun sequence.
ACCESSION   DAOSOR010000217 DAOSOR010000000
VERSION     DAOSOR010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8247
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8636_length_8247_cov_3.904419"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PK710_12705"
     CDS             <1..543
                     /locus_tag="PK710_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10629.1"
                     /translation="FVYPARIVQFLDVGDDWGLQIGLNAVHGTSTFAPEKENRSEAYG
                     ADLLLKWRPIGYGESGFVYVKWTAEGWSRAVQVPQDLWRDIGGYSDLVFGLSKEWNTG
                     VRGEIWRRVGGDAPSADNGRAAFGLDMQRASAQVSYMPSHFSRVRMQYSLENAELLGT
                     SFAGFLQLEVSAGAHGAHTY"
     gene            524..943
                     /locus_tag="PK710_12710"
     CDS             524..943
                     /locus_tag="PK710_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10630.1"
                     /translation="MALIRIDSRRFSEGDNGMNGLNLGAKSACVLGLSLLVSLSACLT
                     EDGKGGEAPIDCGEHGSAHAGHCHCDHGFLFDGTTCVSPDAITEECQEHDDEHADEHE
                     HHHHGACVCPAEGTCPCDGEVESIAGKDYCIPDLHAH"
     gene            complement(956..1402)
                     /locus_tag="PK710_12715"
     CDS             complement(956..1402)
                     /locus_tag="PK710_12715"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOT10631.1"
                     /translation="MSTPDLEQFRKQRLREAGLRATPSRLAVLELLDRSSVPLSHGEV
                     VQVFRAKPWDRATLYRNLLDLVRGRLARRIDVGDRIWRFEIATPHPHDTSHPHFVCTT
                     CKQISCMPGIDLRVIRSEDIPKAVQNQEIDVEIRGLCDICRSRSKR"
     gene            complement(1383..3278)
                     /locus_tag="PK710_12720"
     CDS             complement(1383..3278)
                     /locus_tag="PK710_12720"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOT10632.1"
                     /translation="MQLLAASIVPLLLGPPLVWVLQRARWAAQAIDAFVLTSVAGLVL
                     LSILPGAIAHAGLIAVVAAVLGAILPLLGGSVFAANPAARTITLAVGLVGLFVHASLD
                     GVALAAHDHGQEEGMPLAFAVILHRLPVGLAIWWLVRPKLGLHIALAAVASIAIASVV
                     GFFWAHEAHDWMHGTSLSAFQALVAGSLLHVAAGHSVAPEKPARKEAWQLASAVGGLI
                     GIGIVIVITTAHPLPHAVDTCMSFQRTLLSLASVSAPPLLVAYCLSAVFRVCLPDRHG
                     AWLRGNSALSQTFRGISAGASANTCACTLNPRYESLVRRGIPPSAAIAFLVATPVLGV
                     ASLLVSFPLLGMELTVVRISTGVFLAAVIGLILGKALSRGPGLAASVQPLPTWKPRLQ
                     EGFHYGFVDVPDRTLPWFVAGIVAAAMMEPMLDPAVLARLPSGLDIPLLALFGTPFFF
                     SALGATPVAAVLLHKGVSLGAVIALFLTGPAANISTFALLRALHGRRVAIAFFFILVS
                     AAMAIGYVVHIAFPGKTVSLHALTPSPVHNAALALLALIAVFSLLRQGAHGFVSQVVE
                     LRDHHHHDHEHRGPAPHGHEPQHHDHAHDHEHSCAHHPGHEHPGAHDHDHQHSCGHGH
                     EHEHAGP"
     gene            complement(3369..4178)
                     /locus_tag="PK710_12725"
     CDS             complement(3369..4178)
                     /locus_tag="PK710_12725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HOT10633.1"
                     /translation="MPSVDWLLSKKPIIAMIHVGALPGTPRATESPNALERRAREEAE
                     MYLSAGVDALALENMHDIPYLRGSGGPEIVASMTRIACALRRLTDKPLGLQLLAGGGK
                     EALAVALAAELQFIRVEGFVFAHVADEGYIEACAGELLRYRRMIGAEAIAVFADIKKK
                     HCSHAITADQDIVETAHAAEFFLADGVIVTGRSTGDPADLDELRRVKAASNLPVLVGS
                     GVTVDNVHEYLPHADALIVGSTFKQQGHWARPVDASRVEKFMRRVHQIRQS"
     gene            complement(4308..6248)
                     /locus_tag="PK710_12730"
     CDS             complement(4308..6248)
                     /locus_tag="PK710_12730"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HOT10634.1"
                     /translation="MLGYSYLRLALALPLVAVIGCSSSEDSSPAEQPKPSLDGSVPTD
                     ASDASDASTDVPPDLPIDASEEADALEQPDTQACEPPPILQIDEDFFVDISEESGIQD
                     ENFVENPPVSIPINDHSRLGFADINGDGFDDIVMHSLFPNPKAGIPFEHLVFVNQQDG
                     TFTNVSDASGLRDIQAGFFAFADVDNDGDQDVFAGLDITDLPNMRHQLLLNDGKGVFT
                     VLADSGLEKITQTVASNAVFGDFDGDGDLDLFIANGQTSYLALDLLWLNDGTGVFTNV
                     TATHLKGNRAAPSNGAVACDYDNDGDLDLFVSVYGVSQGGAQNFLFENDGTGKFKDVA
                     VERGFASLPTGNYWTESTGYGTEPEPGKGPGTYVGGNGFGIDCADVNHDGLMDILITN
                     ISHPSSTDASRKWSDPTQLLINQGAAAGWTFKNEFLERGLPFNEGDVDGALMDYDNDG
                     RIDISISRDKKYESSYTEPDQKSWFGLMHQQPDGTFKSVGVKSGINDPQLEWLRMKGA
                     QNHAWSDIDHDGDLDLLVGGRDTGGGRPNFLFKNIVGSQNRWLAIRMIGDGVKVNRDA
                     IGARVTIQDDERVFLREVRATRGMYNSMDTRVLHFGLGILGCSYQIEARWPDGTTVSL
                     DPQAVGMNRYVTLRYPDAIEIP"
     gene            complement(6349..7392)
                     /locus_tag="PK710_12735"
     CDS             complement(6349..7392)
                     /locus_tag="PK710_12735"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOT10635.1"
                     /translation="MGNVRMIRIMTGLVTIPFFIACHGDETETQGHGGPSVDAGADIQ
                     TGADAHNDAGLGGSGGEGQAGNGGTATGGGGSGGSTGGGAGMGATGGSGGSTDASIDT
                     AAPDAISDVMADGIAPSETGPQPSFRDDFDGTEIDSAVWQVATWNEHGGKTGKERCYV
                     QNGHLVLEFVYDTAYYQANGLFLSSAIQTRETFLYGKWEARLKPAEQPGILNSFYTID
                     WGDGSGTKEEIDIEFLTKSFKGSTGQVHYAVHAAGKSSFDTNPDVPLAFNPSADFHIY
                     GFEITPTEIRWTVDGVVMKEYTYVDPYPFITSPYQLKLNAWSQMAGWVGGPPAANTVC
                     KYYIDWIQFTPYP"
     gene            complement(7597..>8247)
                     /locus_tag="PK710_12740"
     CDS             complement(7597..>8247)
                     /locus_tag="PK710_12740"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOT10636.1"
                     /translation="CQMSNNSFEASPGGSGGLYHGGGIPLHLTNSTFSGNTARQGHGA
                     AIQVESSQGTVLHLTSSTFVDNEAKGNAGAIFAGSPVHVLNCTFARNKADYAPAIFRG
                     GNATVSLKNTIFFDNRTDNAYSALACHESFTDEGGNLQWPAIKKSGKEDLPCAAGILF
                     ADPLLSDLADYGGPTLTMALEAGSPAIDLGSDCPEKDQRGAARIGPCDSGAVEFQP"
BASE COUNT         1797 a         2272 c         2487 g         1691 t
ORIGIN      
        1 ttcgtgtacc cagctcgcat cgtacaattt ctcgatgtgg gggatgattg gggattgcag
       61 attgggctca acgccgttca tggcacctcc acctttgccc cggaaaaaga aaaccgcagc
      121 gaagcgtatg gtgccgacct gttgttgaaa tggcggccca ttggctatgg ggaaagcggt
      181 tttgtgtatg tcaagtggac ggcggagggc tggagccggg ccgtgcaggt tccccaggac
      241 ctatggcgcg atatcggcgg atattcggac ctggttttcg gtctgtccaa agagtggaat
      301 accggcgtgc gaggcgagat atggcgaaga gtggggggcg atgcgcccag cgccgacaat
      361 ggccgcgccg ctttcggtct cgacatgcaa cgagccagcg cgcaggtcag ctatatgccg
      421 agccatttct cacgggttcg gatgcaatac tcattggaaa acgcggagct tttgggaacc
      481 agtttcgccg gctttctcca actcgaggtt tccgcgggcg cccatggcgc tcatacgtat
      541 tgattcgagg cgcttttccg agggagacaa tgggatgaat gggttgaatc taggtgccaa
      601 gagcgcttgc gtgctggggc tgtcgctgct tgtttcctta tccgcttgcc ttaccgagga
      661 tgggaaaggg ggcgaagcgc ccatcgattg tggcgagcat ggaagcgctc acgcgggcca
      721 ttgtcattgc gatcatggat ttcttttcga tggcacgact tgtgtctcgc ccgatgccat
      781 caccgaagaa tgccaagagc atgacgatga gcacgccgac gagcatgaac atcatcatca
      841 cggcgcatgc gtgtgtccgg cggaaggaac ctgcccctgt gatggcgaag tggagtccat
      901 cgcgggcaaa gactactgta tccccgactt gcacgcccat tgagccgagg cgcgttcacc
      961 gtttcgaacg cgaacggcaa atgtcgcaaa gtccgcgaat ttccacatct atttcttgat
     1021 tctgcacggc tttggggatg tcttcgctcc gaatgacgcg aaggtcgatc ccgggcatgc
     1081 atgaaatctg cttgcacgtg gtgcaaacga aatggggatg cgaggtgtcg tggggatgcg
     1141 gcgtggcgat ttcaaaacgc cagattcgat cacccacgtc gattcggcga gcgagcctcc
     1201 cgcgtaccaa atcgagcaag ttgcggtaca acgtggcccg atcccagggt tttgcgcgaa
     1261 aaacctgcac gacctcccca tggctcaaag gtacggaaga gcgatcgagc aattccaaga
     1321 cggccagccg cgaaggcgtg gcacgtaaac ccgcctcacg aagtcgttgc ttgcgaaact
     1381 gctcaaggtc cggcgtgctc atgctcatgt ccgtgcccgc atgaatgttg gtggtcatga
     1441 tcgtgcgcgc ccggatgctc atggccaggg tggtgtgcgc atgaatgctc gtggtcatga
     1501 gcatggtcat ggtgttgcgg ctcatgcccg tggggagctg gcccacgatg ctcatggtca
     1561 tgatgatggt ggtcacgtag ttcgaccacc tgggatacga aaccgtgggc gccttgccga
     1621 agcagagaaa aaacggctat caaggcgagc agcgcgagag ctgcgttatg cacggggctt
     1681 ggcgtcagcg cgtgaagtga aacggttttt cctggaaacg cgatatgtac gacgtaacca
     1741 atagccatcg ccgcagaaac caagatgaaa aaaaaggcaa tcgctaccct tcgtccgtgc
     1801 agggcgcgca acaaggcaaa cgtggagata ttggccgcgg ggcccgtcaa gaaaagagcg
     1861 atcacggccc ctagcgatac acccttgtga aggagcacgg cagccacggg cgtggctccc
     1921 agggcggaaa agaaaaaagg tgttccgaat agggcaagca gcgggatatc caggcctgaa
     1981 ggaagccttg ccaacaccgc tggatcgagc atcggttcca tcatggccgc ggccacgatg
     2041 cccgccacga accaaggcaa ggtacggtcc ggaacatcga cgaaaccgta atggaatcct
     2101 tcttgaagcc ggggtttcca cgtgggcagc ggttgaacgg atgcggcaag accggggcct
     2161 cggctcaaag ctttgccaag aatcagcccg atgaccgcgg cgagaaacac acccgtcgag
     2221 atgcgcacga cggtcaactc cattccaagc aaagggaaag aaacgagcag ggaagcgacg
     2281 ccaagaactg gcgtggccac caaaaatgcg atggccgccg atgggggaat gccacgacga
     2341 accaggcttt cgtagcgggg gttgagcgtg catgcgcaag tattggccga ggctcccgcg
     2401 ctaatgcccc gaaacgtttg tgataacgcg gagttgccgc ggagccaggc gccgtggcgg
     2461 tccggaagac acacgcgaaa taccgcggaa aggcaatagg ccacgagaag agggggagcg
     2521 ctcacgctgg cgagggaaag caaggttcgt tggaaactca tgcatgtatc caccgcgtgg
     2581 ggaagaggat gggcggtggt gatgacgatc acgatgccga ttccgatgag cccaccaaca
     2641 gcggaagcca gttgccaggc ctctttgcgc gccggttttt ccggggccac cgagtgtcct
     2701 gccgccacat gcaacagaga gcctgcgacc aaggcttgaa acgccgaaag actcgtgccg
     2761 tgcatccaat cgtgggcctc atgggcccaa aagaatccaa cgaccgaagc gatagctatg
     2821 gacgcaacgg cggctagggc aatgtgcaac cccaacttgg gacgcacgag ccaccagatg
     2881 gcgagcccca ccggaagacg gtgaagaatc accgcgaagg caaggggcat tccctcttct
     2941 tggccgtgat cgtgagcggc aagagcgact ccatcgaggg aagcgtggac gaacaagcct
     3001 accagaccca ccgccagggt aatggttcgc gccgcgggat tggcggcaaa gacagaaccg
     3061 cccaacagcg gaaggatcgc ccccagcacc gccgccacca cagcgatgag accggcatgg
     3121 gcaatggctc ccggaagaat gctgagaagg acgagccccg ccacggaagt aagcacgaag
     3181 gcatcgatgg cctgggcagc ccagcgagcg cgctgcagaa cccaaaccag tggtggtccg
     3241 agcaaaaggg gaacgatgct ggcagccagt aattgcataa gagttgcagg tggccttatc
     3301 accggaccta aggcatgaca atgagggatg caaaggcagg aggcttgccg aggggcctgg
     3361 atgagcggct agctttgacg gatttgatgg actcgccgca tgaatttttc gacgcgagag
     3421 gcatcgacgg gtcgtgccca gtgtccttgc tgcttgaagg tggaaccgac gatcaaggca
     3481 tccgcgtgcg gcaggtactc atgcacgttg tcgacggtaa cgcccgagcc gacgagtacg
     3541 ggaagattcg aggctgcttt tacacgccgc aattcatcga gatctgcggg atctccggtc
     3601 gaacgtccgg tgacgatcac cccatcggcc aggaaaaact ccgccgcgtg ggcggtctcc
     3661 acgatgtctt gatctgccgt gatggcgtgg gaacagtgtt tctttttgat atccgcgaac
     3721 accgcgatgg cctcggcgcc gatcatccga cgataccgaa gcaactcacc cgcacaggct
     3781 tcgatatagc cttcgtcggc gacgtgggcg aagacgaatc cttcgacacg gatgaactgc
     3841 agttcggcgg cgagggcgac ggcgagggcc tcttttcctc ctcctgccag gagttggagt
     3901 ccaagaggtt tgtccgtgag gcgacgcagg gcgcaggcga ttctcgtcat cgaagcgacg
     3961 atttccggtc ccccgctgcc tcgtaagtat ggaatatcat gcatgttttc tagcgccagg
     4021 gcatcgactc cggcggatag gtacatctcg gcttcttcgc gggcgcgacg ttcgagggcg
     4081 tttggcgatt cggtggcgcg tggcgttcct ggcaaagccc cgacatgaat catcgcgatg
     4141 atgggttttt tcgatagcaa ccaatcgacg cttggcattt tttcgttccg gttcgtgagg
     4201 ggatgactgc gaagccctta gcacgggtgt tgctcatggg tcgaacgcga tggtgtgggc
     4261 atcgaaaagg agcgagatat ttcgttgtga aaagcgagag gaagggatca aggaatttcg
     4321 atcgcgtcgg ggtagcggag ggtcacgtat cggttcatgc ccacggcttg ggggtcgagg
     4381 gaaaccgtgg ttccatccgg ccaacgggct tcgatttgat aggaacaacc gaggattcct
     4441 agcccaaagt gaagcacgcg cgtatccatc gaattgtaca tgcctcgggt ggcgcgcact
     4501 tcacgcagga aaacgcgctc gtcgtcttgg atggtgacac gcgcgccaat cgcatcgcgg
     4561 ttgaccttca ccccatcacc gatcatgcga atcgccaacc aacggttttg ggatcccacg
     4621 atatttttga acagaaaatt cggtcttccc ccgccggtgt cccgacctcc caccaacaag
     4681 tcgaggtcac catcgtggtc gatgtccgac cacgcatgat tttgcgcccc tttcattcga
     4741 agccattcga gttgtggatc gttgatcccg cttttgactc ccacactctt gaacgttcca
     4801 tcgggttgtt gatgcatgag tccaaaccag gacttctggt ctggctccgt gtacgaactc
     4861 tcgtattttt tatctctgga aatggatata tcgatgcgac catcgttgtc gtaatccatc
     4921 aacgcgccat ccacatcccc ttcgttgaaa ggaagtccgc gttcgagaaa ctcgtttttg
     4981 aacgtccagc ccgccgcggc cccctggttg atcaagagtt gcgttggatc cgaccatttt
     5041 ctcgaagcat ccgtcgacga aggatgggaa atattggtga tcagaatatc catgagccca
     5101 tcgtggttga cgtcggcaca gtcgatgcca aacccattgc cgccgacgta ggtccccggc
     5161 cccttgcccg gctccggctc ggttccgtag cccgtgcttt ctgtccaata attgccagtc
     5221 ggaagacttg caaacccgcg ttccaccgca acatccttga attttccggt accatcgttc
     5281 tcgaaaagga agttctgggc tccaccttgg ctcacaccat atacggatac aaaaaggtcc
     5341 agatcgccat cgttgtcata gtcgcaagcg accgcgccat tcgaaggcgc ggcgcgattt
     5401 cctttcaaat gcgttgccgt cacgttggtg aacacaccgg tgccgtcgtt gagccacaaa
     5461 agatccaacg ccaagtaact cgtttgccca ttggcgatga atagatcgag atccccatcg
     5521 ccatcgaagt caccgaatac ggcattggag gctaccgtct gcgtgatttt ttccaacccc
     5581 gagtcggcca ggaccgtgaa tacacccttg ccgtcgttga gcagcaattg gtgccgcatg
     5641 ttgggcaagt ccgtgatgtc gagtcccgca aaaacatctt gatcgccgtc gttgtccacg
     5701 tccgcgaacg caaaaaaacc tgcctggatg tcgcgaagcc cggaggcatc gctcacattg
     5761 gtaaacgttc cgtcttgctg gttgacgaag acgagatgtt cgaaaggaat gcccgccttg
     5821 gggttgggaa agaggctatg catgacgata tcgtcgaaac catcgccatt gatatccgcg
     5881 aagccaaggc ggctatggtc gttgatggga atgctaaccg gtggattttc cacgaaattt
     5941 tcgtcttgga tgccgctttc ctccgagatg tccacgaaaa aatcctcgtc gatttgcaag
     6001 atggggggag gttcgcacgc ttgggtatcc ggttgttcca gcgcatccgc ctcctcggag
     6061 gcgtcgatag gcaaatccgg cgggacgtcg gtagaggcat cggatgcatc ggatgcatcg
     6121 gtgggcacgg agccatccag cgaaggcttg ggttgttccg cgggagacga atcctccgaa
     6181 gaagagcatc cgatgacggc aacgaggggt agcgccaacg caaggcgaag atatgagtat
     6241 ccaagcatgc ggcaagcata tcattgattg cgcttggttc cccggaggtt tcgtgctcgg
     6301 tgacggcctt attccccggc cactgccggt tgaacgctac gatggggctc aaggataagg
     6361 agtaaactga atccagtcga tatagtactt gcagacggta ttggcggctg ggggcccgcc
     6421 cacccaaccg gccatttggg accaggcatt gagcttcaat tgatagggcg acgtgatgaa
     6481 agggtagggg tccacgtacg tgtattcttt catgacgacc ccatcgaccg tccatcggat
     6541 ttcggtgggg gtgatttcga aaccgtagat atgaaaatca gcggaggggt tgaacgcgag
     6601 gggtacgtcc ggattggtat cgaaactgct ctttcctgct gcatgaaccg cgtaatgcac
     6661 ctggcccgtc gaacccttga acgatttggt gagaaactcg atgtcgattt cctctttggt
     6721 tccggagccg tcgccccaat caatcgtata aaaggagttg agaattccgg gttgctcggc
     6781 gggtttgaga cgggcttccc atttgccgta aagaaaggtt tctcgggtct ggatggcgga
     6841 actgaggaaa aggccgttcg cctggtagta agccgtgtcg taaacgaatt ccaagacaag
     6901 atggccgttt tgcacgtagc agcgctcttt gcccgtcttt ccaccatgct cgttccaggt
     6961 cgcgacctgc cagacggcgg aatcgatctc cgtaccgtcg aagtcatcgc gaaatgatgg
     7021 ttgaggccct gtttcggaag gggctatgcc gtcagccatc acgtcggaaa tcgcgtcggg
     7081 agccgcggta tcgatgctgg cgtcggtact ccccccgctt ccacccgttg cacccatgcc
     7141 tgcgccgccg ccggtcgatc cgccgctacc gccacctccc gtggccgttc caccgttgcc
     7201 tgcttgtcct tcgccgccgc tgccacccag gcctgcatcg ttgtgggcat ccgctcccgt
     7261 ttgtatatcc gcgcctgcat cgaccgaagg accaccatgg ccctgggttt ccgtctcatc
     7321 accatgacaa gcgatgaaaa atgggatcgt cacaaggccg gtcatgatcc gaatcatccg
     7381 tacgttgccc atcctccacg ctcctttcca tcggccccaa tttcgacccg ctcacctgca
     7441 ggaatgagtt tatcccacgg tttgccggtt gaccatccgc aatcttggct gggctgaaac
     7501 ggcgcactgc ggcgaaaggg atgtggctgc gggaatcata cagacgctag gtggaatttc
     7561 atggggagtc tgatgggagg gacgggcgaa tcgaatttag ggttggaatt cgacggctcc
     7621 tgaatcacag gggcctatcc gagcggcccc ccgctggtct ttttctgggc aatcggaacc
     7681 caaatcgatg gctggactgc ccgcctcgag cgccatggtc aacgttgggc cgccataatc
     7741 cgcgagatca ctgagcaaag gatcggcgaa aaggattccc gccgcgcagg gaaggtcttc
     7801 tttgccgctt tttttgatgg ccggccattg aaggttgccc ccttcatccg taaaagattc
     7861 gtgacacgcg agggcgctat aagcgttgtc ggttcgatta tcgaaaaaga tggtgttctt
     7921 gagcgatacg gtggcgttgc cccctcggaa aatggcgggg gcataatcgg ctttgttgcg
     7981 cgcaaacgta caattgagga catgcacagg actgcccgca aaaatggctc cggcgtttcc
     8041 tttggcttca ttatcgacaa acgtgctgct cgtcagatgc aaaacggttc cctgggacga
     8101 ttcgacctga atggcggcgc catggccttg acgagcggtg ttgccggaga acgtcgaatt
     8161 ggtcaggtgt agcgggattc caccaccatg ataaagcccc cctgagcctc cggggcttgc
     8221 ttcaaaggag ttgttgctca tctggca
//
LOCUS       DAOSOR010000218         8227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8662_length_8227_cov_5.083700, whole genome shotgun sequence.
ACCESSION   DAOSOR010000218 DAOSOR010000000
VERSION     DAOSOR010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8662_length_8227_cov_5.083700"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PK710_12745"
     CDS             <1..235
                     /locus_tag="PK710_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10637.1"
                     /translation="AGTFTYPDASAAQTASTSLEQIAQLSSYARLLSLLGIQPPIQDL
                     KVRVVDQDVQFIAAINVQGASSLMEWWASSLRR"
     gene            246..932
                     /gene="cmk"
                     /locus_tag="PK710_12750"
     CDS             246..932
                     /gene="cmk"
                     /locus_tag="PK710_12750"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOT10638.1"
                     /translation="MTSYHVVTIDGPAGAGKSTLACALASRLGYVYLDTGAIYRALAL
                     ACQRASVPWCSEAQVHQVAIDLVSRNRIRFLKQQGDQRLYLDDEDVSDAIRTPEVSSG
                     ASTVSAHPDVRTALLSLQREVANQSNVVVEGRDTGTVVFPKAEVKFFLTASLEQRAKR
                     RFEELTAKGVVVNYDDTLIAMRERDERDEKRAAAPLRRADDAIELDTTPMTIEQVLGF
                     MIEHVRKRIL"
     gene            939..1847
                     /locus_tag="PK710_12755"
     CDS             939..1847
                     /locus_tag="PK710_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10639.1"
                     /translation="MTGVARVTIWAGFLIGALACTPSPPQDIPKGWTPTLEQLIPADV
                     DLVVRVDLETTRQQGVYTLFLSLVEQSGLSSSVFDLIAGCGSDATTATFALRYGPDVT
                     DGDLMVVFAGSRQSYGIPCGAVGWKSAGKKRDLQLFEPQSSSSERTAAALMIRSDSGQ
                     IMLVTPGQLDSLIRLLKDGPDMDRTTISDHKATTTIHIHRPMVPEAWKSKAPSLARVA
                     RGLRQAQVRLLVARAQEVSVQADFVYHDDSAALQAGQLLERVRDALLAADSEVSNIIG
                     QSAHATLRGDNLHVEFLVPLPTADSP"
     gene            complement(1868..2116)
                     /locus_tag="PK710_12760"
     CDS             complement(1868..2116)
                     /locus_tag="PK710_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10640.1"
                     /translation="MTHHATSQATPTVSSTIPGEFRPVEQAAPQRDGTVLMIEAYAFV
                     WMILMIFVLTGYLRQRRLDARIARLESAIDRVIEREQP"
     gene            complement(2119..2853)
                     /gene="ccsA"
                     /locus_tag="PK710_12765"
     CDS             complement(2119..2853)
                     /gene="ccsA"
                     /locus_tag="PK710_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOT10641.1"
                     /translation="MKSCASARSSARLLDIAFVSLLTLATLGVLITVHLIFLRTPVEA
                     QMGIVQKIFYFHVPSAYAMYLGATACFLGSTAYLFRISDRRDALARAGAEVAVAFGAV
                     VLATGPLWAVKAWGHYWTWEPRLTTSLLSVLVYVAYLLLRSFGGGGEGERKFAATLGF
                     LGALNLPLIHFSVRKWSGQHPEVVTGQGGGLQHVDMRITFIIAMLTFTFLAGTLLWFR
                     FRLWSTEARLRRLEKEALEFNVGEES"
     gene            complement(2895..3515)
                     /locus_tag="PK710_12770"
     CDS             complement(2895..3515)
                     /locus_tag="PK710_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10642.1"
                     /translation="MRQSVQPHDLIAMLRRLLKMAPPDSEDALFAHRHLAELTVETEP
                     WKAALSARRLIAANPNDDQGWALLALAMSLMSHYRAAERAYRKAIAISPRNPWYAHNL
                     GHLLDVVLDRPNEGLPLLAFAHRMEPQETEIASSYAHVLGRVGKTEDARKLLRRALRQ
                     GGTSEQRALLTWLDEACDGRPSKSRRRKTKTSTENSSSSHRKNRRA"
     gene            complement(3617..5812)
                     /locus_tag="PK710_12775"
     CDS             complement(3617..5812)
                     /locus_tag="PK710_12775"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT10643.1"
                     /translation="MTEASEHVQIEDTPQLPSFEQMPLNDDVKHALAEMGYTHPAPVQ
                     HAVYDPAVRGLDVVVQARTGTGKTAAFGIPLVDRMIKRSVRSPQALVLTPTRELALQV
                     SREIERIGSKRDIRSATVYGGAPIQRQIDALQGGAQIVVGTPGRVLDHLRRGTLQTQH
                     LRILILDESDEMLSMGFERELNAIVDHLPEPRQTLLFSATLPPEIVKMASSRLHDPRF
                     IYLSGDQVGALEILHFVYFVQKDKSDALLRLLEVENPESALIFCNTRDDTERVAGMLQ
                     RAGYDADWLNGDLPQSDREKVMTRSKEGHLRLLVATDVAARGIDISHLTHVINFDFPD
                     SAETYVHRTGRTGRMGRTGTALSLITPHDIGALYYLRLTYKIRPFEKQLPSEGELKTR
                     AHADVISLLNEAFSSQPHPDDMALARRLLTHDRSEFIIAGLLRNHLGDRLKSEEEAAE
                     ARRAKRPAPVKVHAPDESSDEKIREASADASRKQHTPPLPPSSEQRPVEEPEQDEDSV
                     DSPPTQEYPRAEDQPKDDDDLPTDPPAEPEVDLGHAVESDANDDSPERDDSAHDSHGF
                     EDEEPTGKHRTVPEMDLPRYEISPMTQEDLDLIRDIQQKQQQEQPVGHAALADWFPPA
                     EDNDDQPILPEKGAELPPSEDDFDEQDELEVFVNVGRRDGVRPGDFVAVLAEARGIQR
                     ADIGRIRIRDKHTFIAVRAEVIDDAVRAIQGRAFGDRVATAARARMRED"
     gene            complement(5943..6488)
                     /locus_tag="PK710_12780"
     CDS             complement(5943..6488)
                     /locus_tag="PK710_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10644.1"
                     /translation="MTIHRAWGHKALMMQDRQDRQERNARKKGRRGVMIGYFVFVVVV
                     IAVCTVQITTQAVGRLGGISSSTPIDCDRGIESLMAAVERARSAAATSHETETKAVAL
                     FRASLEPEWSRRGDIERACAGQPDRLEALDAVIHLGFAEEHAVRRDAIELSDIRRKAA
                     SLLRRPASPSLSSSTQRESWP"
     gene            complement(6556..>8227)
                     /locus_tag="PK710_12785"
     CDS             complement(6556..>8227)
                     /locus_tag="PK710_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10645.1"
                     /translation="RQAFADRAGRVMGLAAETASAQMGMPGLGEDGPPPAAGATPTAQ
                     PNAPATHAASGGEELGVLPSLEASIPEDPLKLSKVVKKTIGSDADNEFELGRDTETTY
                     SWYGPYYQEKSGQYRFRTVFPFWLEREQPKDTASFISPFYYRRRSPNVDADVVFPLYW
                     NMRENESRTHVIGPLAWRKAPGESDHWLAPLVFQGTRKDGSYLHIPPLLTFTSQTHTS
                     GFNLVGPGYCSWKGGSSCSLSSARDIDFGIAPLYFYGRSETSEYELIPPLLHYYRYDD
                     IGEKSLNLWGPLLWSHTRERSAFHVLPLFYHLWGEDEDHVTLFPVFHYGHKGRSSLFV
                     NPLYMTSTGDKGEKTFVTWGYARYRGRTELDMITPLYWHYHDPDIGEDTHLLLPLLYR
                     SVSPRQRDLAVFPLWGHFNRFGIKKTTWITPLFQHSRGLTGWETNIHPVLYMGRNQDR
                     THLVVAPIFWDFASPKSRSTVAFPVFWRFDDGNSVSQLLLNTYYQERKLRTGLDWQFH
                     FFPAFSYGETPDGHWWKVMYGLAGYTRRGEMAKIYAAWIPFLLSGDDD"
BASE COUNT         1825 a         2295 c         2294 g         1813 t
ORIGIN      
        1 cgctggtact ttcacctatc cggatgccag cgccgcccaa acggcaagca cctccctgga
       61 acaaatcgcg caactttcgt cgtatgcccg actgttgtcg cttctcggca ttcaaccgcc
      121 cattcaagat ttgaaagttc gcgtcgtcga tcaggatgtt caattcatcg cggcgatcaa
      181 cgttcaggga gcaagttcct tgatggaatg gtgggccagt tcgttgcgtc gatgaggagg
      241 aaaccgtgac atcgtatcat gtcgttacga tcgatggtcc tgcgggagcg ggaaagtcca
      301 cactcgcgtg tgcgttggcc tctcgtcttg gatacgtata ccttgacacc ggtgccatct
      361 accgtgccct cgctctggct tgccagcgcg cttcggtacc atggtgttcg gaggcccagg
      421 ttcatcaagt ggcgattgat ttggtgagcc gcaatcgaat tcgattcttg aaacaacaag
      481 gggaccaacg actgtatctc gatgacgaag acgtctccga tgccattcgt actccggaag
      541 tgagtagtgg cgcgagcacg gtgtcagccc atcccgatgt tcgcacggca ttgctgtcgt
      601 tgcagcggga ggtagccaac caaagcaatg tggtggtgga agggcgagat accggcacgg
      661 tcgtatttcc aaaagccgag gtgaagtttt ttctaacagc ttctctcgaa cagcgcgcca
      721 agcggcgttt cgaggaactg acggcaaaag gtgtggtcgt caactacgac gacacactga
      781 tcgcgatgag ggagcgagac gaacgcgatg agaaacgcgc cgcagctccg ttgcgtcgtg
      841 ccgacgatgc catcgagttg gacaccacgc ccatgacgat cgaacaggtg cttggtttca
      901 tgatcgagca tgttcgcaag cggattttat aacgatccat gacaggtgtt gcgcgtgtca
      961 ccatttgggc aggctttctg atcggtgcct tggcatgcac gccgtctcca ccccaagata
     1021 ttcccaaagg atggacacca acgctcgaac agttgattcc ggcggatgtc gatctcgtgg
     1081 ttcgggtcga cttggaaacg actcgccaac aaggcgttta cacgttgttt ctgtctttgg
     1141 tggaacagtc gggtctatcg tcatccgtat ttgatctgat cgcaggctgt gggagcgacg
     1201 ccacgaccgc tacctttgcc ttgcgctacg gtcccgatgt caccgatggc gatctgatgg
     1261 tcgtgtttgc tggttcgagg caatcctacg ggattccttg cggcgccgtt gggtggaaat
     1321 ctgcggggaa gaaacgtgat ctacaattgt tcgaaccgca atcgtcatcg tcggagcgta
     1381 ccgcggccgc cctcatgatt cgttcggatt cgggacagat catgctggtg actccaggtc
     1441 aattggactc gctgatccgt ttgctgaaag atggaccgga tatggatcgc actactattt
     1501 ccgaccataa ggcgacgaca acgattcata tccatcgtcc gatggtgcca gaagcctgga
     1561 aaagcaaagc gccgtcgctt gcacgagtgg cgcgaggtct gcgtcaggcg caagttaggt
     1621 tgttggtggc tcgtgcgcag gaggtgtccg tgcaggctga tttcgtttat cacgatgatt
     1681 cggcggcgct tcaggcagga caactcctcg aacgtgtgcg tgacgcgctc ctggcggccg
     1741 actcggaagt gtcgaatatt attggacaat ctgctcacgc caccctgcgt ggcgataacc
     1801 tccacgtaga attcctcgtg cctttaccca ctgccgattc accgtagccc gtcaggaatc
     1861 gtcgttctca aggttgttcg cgttcgatga cgcgatcgat cgcactttcc aatcgcgcga
     1921 tgcgggcgtc gagacgacgc tgtcgtaggt atcccgtcaa gacgaagatc atgagaatca
     1981 tccaaacgaa ggcgtatgct tcgatcatca gcacggtgcc atcgcgctgg ggtgcggctt
     2041 gttctacagg ccgaaactcg ccaggaatgg ttgaagatac cgtgggagtg gcttgcgagg
     2101 tcgcgtggtg agtcatccct acgactcctc cccgacattg aattcaagag cttctttttc
     2161 caagcggcgt aggcgtgctt ccgtggacca aaggcgaaac cgaaaccaaa gaagcgttcc
     2221 tgccaggaag gtaaacgtca gcatggcgat gatgaaggtg attcgcatgt ccacatgctg
     2281 aagacctcca ccttgtcctg tgacgacttc gggatgttgt ccgctccatt ttcgaacgga
     2341 aaaatggatg aggggaaggt tcagcgctcc aagaaaacca agggtagccg cgaacttacg
     2401 ctcgccctct ccacctccgc caaaggaacg taacaacagg taagcaacgt aaaccaacac
     2461 cgacaatagg gaggtcgtca gccgaggctc ccatgtccaa taatgtcccc aagctttgac
     2521 ggcccataaa ggtcctgtgg caagaacgac ggcgccaaac gccacggcca cttctgcacc
     2581 cgcgcgcgcc aatgcatcgc gacggtcact gattcgaaaa agataggcag tcgaaccgag
     2641 aaaacacgcg gtcgcgccca ggtacatggc gtacgcggaa ggtacgtgga aataaaatat
     2701 tttttgaacg attcccatct gcgcttccac cggtgtgcgc aaaaaaatca agtggaccgt
     2761 gatgagaacg ccaagcgttg ccaacgtgag taacgaaacg aaagcgatat cgagcaagcg
     2821 agctgaggat cgagcagatg cacaggactt catgaggaca tgcatttcga acgcgcgagg
     2881 cccgaacctt cgtatcaggc cctacgattc ttgcggtgag atgaggagga gttctccgtc
     2941 gaagtcttcg ttttacgacg ccgtgatttg cttggccttc cgtcacatgc ttcgtcgagc
     3001 caggtaagaa gcgcgcgctg ctcactcgtt cctccttgtc gaagggcacg ccgcagtagc
     3061 tttcgcgcgt cttcggtttt tccgacacgc cctaacacat gcgcgtaaga ggaagcaatt
     3121 tcggtctcct ggggctccat tcgatgggca aaggcgagca aagggagacc ttcgttgggc
     3181 cgatcgagca caacatcgag caaatggcct agattatggg cataccaagg attccggggc
     3241 gagatcgcga tcgccttgcg atacgcgcgt tcggcagcac ggtaatggga catgaggctc
     3301 atggcgagtg ccagcaatgc ccagccttga tcgtcattgg gattcgctgc aatgagacga
     3361 cgagccgaaa gtgcggcttt ccatggttcc gtttcgaccg tcaattccgc gagatgccga
     3421 tgcgcgaaaa gggcatcctc gctatcggga ggagccattt tcaagagacg ccgcagcata
     3481 gcgatgaggt catggggttg cacggattgg cgcattacgc actctgcact gtgtcggagc
     3541 tttttgacct ccaggtcttg cgcgtccttg cccataatca atagatacct tcgcaatagc
     3601 gttcgtgcaa cgcgtactag tcttctcgca tcctggccct ggcggcggtg gctacacgat
     3661 cgccgaaggc tcgcccttga atggcgcgca cggcatcgtc gatgacttca gctcgaacag
     3721 cgatgaacgt atgcttgtcc cgaattcgaa tgcggccaat atccgctcgt tgaatccctc
     3781 gcgcctcagc aagaacagcc acgaaatctc caggacgcac cccgtctctg cggccgacgt
     3841 tgacgaacac ctcgagttcg tcttgctcat cgaaatcatc ctccgaaggc ggtagttccg
     3901 ctcctttttc tgggagtatc ggttggtcat cgttgtcttc ggcaggagga aaccaatcgg
     3961 ccaacgcagc atggcccacg ggttgttctt gctgttgctt ttgctgaatg tcccggatca
     4021 aatcgagatc ctcctgtgtc atgggagaga tctcgtatcg cggcaaatcc atttcgggca
     4081 cagtacgatg ctttcctgtt ggttcctcgt cttcgaaacc atgcgaatca tgagcggaat
     4141 catcgcgctc gggggagtcg tcgttcgcat cactctcaac cgcgtgcccc aaatcaactt
     4201 ccggctcggc cggagggtcc gtgggcaagt catcatcgtc tttgggttga tcttcagccc
     4261 ttggatactc ctgggttggc ggcgaatcga ccgaatcttc gtcctgctcc ggttcttcga
     4321 cgggtcgttg ctctgacgac ggcggaagag gaggggtgtg ttgtttcctg gaagcgtccg
     4381 cagaagcttc gcgtattttt tcatcagagg attcgtcagg agcatggacc ttgacaggcg
     4441 cgggccgttt ggctcgacgc gcttctgcgg cttcttcctc ggatttcagc cgatctccga
     4501 gatggttgcg caacaatccg gcgatgatga attcacttcg atcgtgggtg agaagcctac
     4561 gcgcaagcgc catgtcgtcg ggatgcggct gcgaagaaaa cgcttcattc aacagggaaa
     4621 tcacatccgc gtgagcgcgc gttttcaact ccccttccga cggaagctgt ttttcgaagg
     4681 ggcgaatttt ataggtaaga cgaagataat agagcgcgcc gatatcatgg ggcgtgatca
     4741 aactcagggc ggttcccgtt cgtcccatcc ttcctgttcg ccctgttcga tgaacgtagg
     4801 tttccgccga gtccgggaag tcgaagttga tgacgtgagt cagatgcgag atgtcgatgc
     4861 cacgagcggc aacgtccgtg gccacgagca agcgaaggtg accttctttg ctgcgcgtca
     4921 tcaccttctc tcgatcggat tggggaagat ccccattgag ccaatcggca tcgtagccag
     4981 cgcgttgaag cataccggcc acccgctccg tgtcatcgcg cgtgttgcaa aatatgagag
     5041 cactttcagg gttttccacc tctaacagcc gcaacaaggc atcgcttttg tctttttgaa
     5101 cgaagtacac gaagtgcaga atctcgagag cccctacctg atcacccgat aagtaaatga
     5161 atcgcgggtc atgcaatcgc gatgatgcca tttttacgat ttcgggaggt aacgtcgcgg
     5221 aaaacaaaag cgtctgacgc ggctccggaa gatgatccac aatggcattg agttcccgtt
     5281 cgaaccccat cgacagcatc tcgtcggatt cgtccaaaat caatatgcga agatgctggg
     5341 tttgcagcgt tccccttcga agatggtcga gaacacgccc aggcgtaccc acgacgattt
     5401 gagcgccgcc ttgcagcgcg tctatctgac gttgtatggg cgcgccgcca tatactgtgg
     5461 cagaacggat gtcgcgcttg cttccaatcc gttcgatctc tcgcgatact tgtaacgcca
     5521 actcccgagt cggtgtcagc accaacgcct ggggtgatcg gacactgcgc ttgatcatcc
     5581 ggtcgaccaa agggataccg aatgccgcag tttttcctgt tcccgtccta gcttgcacca
     5641 ccacgtccaa cccgcggacc gctggatcgt atacggcatg ctgtacgggc gccggatgcg
     5701 tgtatcccat ctcggccaag gcatgcttga cgtcgtcatt caggggcatc tgctcgaacg
     5761 aaggcagttg cggtgtatct tcaatctgaa cgtgttcgga agcttcagtc ataggttttc
     5821 tcgcgacgat cgaggtcgtt ggaagtaaaa gacagcacgg gacaaaacca gccgtagacc
     5881 gtagctagcc cttggcgtcg atggatcgca agaggctttg ctgtaaaccg gcgaaaagat
     5941 ggttagggcc aagattctcg ttgcgttgaa gaggataggg aaggggaggc gggtcgacga
     6001 agcaaggatg cggctttgcg gcggatgtcg gataactcga tggcgtcgcg ccgcacggcg
     6061 tgttcttcgg caaaacccaa gtgaatgacc gcatccaagg cttcgagacg gtccggttgg
     6121 cccgcacagg cgcgttcgat atctccgcgg cgagaccact ctggttcgag agacgcgcga
     6181 aatagcgcga ctgctttcgt ttcggtttca tgggacgtgg ccgccgctga acgggcacgt
     6241 tccacggcag ccatcaacga ttcgattcct cggtcacaat caatcggcgt gctcgacgaa
     6301 attccaccaa gccttcccac cgcctgcgtc gtaatctgca cggtgcaaac ggcaatgacc
     6361 acgaccacaa acacgaaata cccgatcatc actccacgac gacccttttt tcgggcgttt
     6421 cgttcttgcc ggtcctgccg gtcttgcatc atcaaagcct tgtgacccca agcacgatga
     6481 attgtcaacg gtattagaaa taccgcgtcc agtccctagc tttcgacaaa gcaagcgcga
     6541 gtcacgcatc cgcgatcaat cgtcgtctcc gctcaacaag aacggaatcc aggccgcata
     6601 tattttcgcc atctctccgc gtcgcgtgta gccagcaagc ccgtacatca ccttccacca
     6661 atgcccatcg ggcgtctccc cataagaaaa cgccgggaag aagtgaaact gccagtctag
     6721 ccccgttcgc aactttcgct cttgataata ggtattcagc agcaattggg agacggaatt
     6781 cccatcgtcg aaccgccaaa acaccgggaa cgcaaccgtg ctcctgcttt tgggcgaagc
     6841 gaaatcccag aatatcggcg ctacgacgag atgggtgcgg tcttgattac gccccatgta
     6901 tagcaccgga tggatattcg tttcccatcc cgtaagccct cgagaatgct gaaatagcgg
     6961 cgttatccac gtcgtttttt tgatcccgaa acggttgaag tgcccccaaa gtggaaagac
     7021 cgccaagtcc cgctgacggg gactcaccga tcggtaaagc aacggcaaca acaggtgcgt
     7081 gtcctcgcca atatccggat catgataatg ccaatacaat ggcgttatca tatccaactc
     7141 cgttcgaccc cggtaacgtg cgtaacccca cgtaacgaaa gtcttttctc ccttgtcccc
     7201 cgtcgaggtc atatacagcg ggttgacgaa taaggaagag cgtcccttgt gtccgtaatg
     7261 gaaaaccgga aatagggtga cgtggtcttc atcctctccc catagatgat agaacaacgg
     7321 aaggacatga aacgcgctgc gttcgcgcgt gtggctccac aacaaggggc cccacaaatt
     7381 caacgatttt tcgccgatgt catcgtatcg gtaataatgc aaaagcggag gaatgagttc
     7441 gtattccgag gtttcggagc gaccgtagaa gtacaggggc gcgattccga aatcgatgtc
     7501 ccgcgcggaa ctcaggctgc acgaagatcc ccctttccac gagcaatacc ccggccccac
     7561 caagttgaat ccactggtat gggtctgcga ggtgaaggtg agcaaaggcg gaatatgtaa
     7621 ataagatccg tcctttcgtg tcccctgaaa gaccaaaggc gcgagccaat gatccgattc
     7681 cccgggcgct tttcgccacg cgagaggtcc gatcacatgg gtacgactct cattttcgcg
     7741 catgttccaa tacaagggaa agacgacatc cgcatccacg ttgggactgc gcctgcgata
     7801 ataaaaaggt gagataaaag aggccgtgtc tttgggctgc tcgcgttcga gccagaaagg
     7861 aaacaccgtg cgaaaacggt attggccgct tttttcctga taataagggc cgtaccagga
     7921 ataggtggtc tcggtatcgc gtcccagttc gaactcatta tccgcgtccg aaccgatcgt
     7981 ctttttcacg accttggaca gcttcagcgg gtcttctgga atcgaggctt cgagagaggg
     8041 aagcactcca agttcctctc cacccgaagc cgcatgcgta gccggtgcat tgggctgcgc
     8101 tgtaggagtc gcccccgcgg caggtggagg cccgtcctcc ccaagacctg gcattcccat
     8161 ctgcgccgag gctgtctccg ctgccagccc catgacgcgc ccggcgcggt cggcgaaagc
     8221 ctggcgc
//
LOCUS       DAOSOR010000219         8226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8665_length_8226_cov_4.155795, whole genome shotgun sequence.
ACCESSION   DAOSOR010000219 DAOSOR010000000
VERSION     DAOSOR010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8226
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8665_length_8226_cov_4.155795"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1743
                     /locus_tag="PK710_12790"
     CDS             <1..1743
                     /locus_tag="PK710_12790"
                     /inference="COORDINATES: protein motif:HMM:NF040163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5722 domain-containing protein"
                     /protein_id="HOT10646.1"
                     /translation="VDAESGVADGSAGTGGSAGVGGSAGVGGTSGEAGAAGKGGAAGA
                     GGIGGSGGAAGSAGTGGSAGTAGSGGTAGAGGTGGSGGSGGNGLPYPTRNAYKIKGLQ
                     PDFWPNPDEISGNNTGGVAMNLVWAHWEPSKKAPPCASNEEEFDGHCFVVNSKVDKAI
                     ADWTARGLVVTAVVYGVPAWARISSGCSPVSPGFEIFCAPKNANDYGRFTGMLARRYN
                     GLHGHGRIADFVIHNEVNANDWFDIGCGQGKPCDPKAWMDTYAANYNAAYDAVKAEQT
                     EAKILISLEHHFGSSYDAPAANNPLLSGQTMLTGLASRVGSRAWRVAYHPYPPNLLAP
                     AFGPDDWPRVTYGNLGTLAGWLRKTFPNTPSAWEIQLTESGVNSLAPQSSQAAQADGV
                     CRSLRNVLGTPGIESYIYHRMTDHPAETAAGLGLGLHDDKGHAKEAWAVWALANRNDL
                     NPPKLSCGFEHLPYTRLTRSYHSSRGHWASSRIAPSGFKTEQSWRLLRAPKAGTKMLY
                     ECLVGSHNMLSTSVNCENQHPMGPVGYIYETKQSGTVALHRCRIGAGSDHFVSTDPGC
                     EGQKFEQLLGYVLP"
     gene            2053..3084
                     /locus_tag="PK710_12795"
     CDS             2053..3084
                     /locus_tag="PK710_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10647.1"
                     /translation="MRDPTRFERLLLGLPEDLAARVRALLPSTHFEAAHAHLQRFAAA
                     MRIGSLATGTWRGQDISGEDIHIHVQAESVAFEDATVQLHVKVGLGFQVPSLRKPKVY
                     KLGPVEESVSTPKLPRIDISSLAGGLSTTFTLSSVEADEVIADVAPIANANAGTIVVE
                     DIDLQELSIPQGPSASLQLDHAKVERAVLREASVANCKTARAGLAEPLTASDVVVKGA
                     RIVNGRATASGQDLDLGFEVQLPALTIKTFPSMPAAIDRLVTRLSVRIEPKVVFQVGK
                     LKLEGMTLTTRVGTLRVKALSVPLQMHELTMEDLHVQEAKAEGLEIHTRDEAQEADDA
                     KGLLPSQEE"
     gene            complement(3203..4558)
                     /locus_tag="PK710_12800"
     CDS             complement(3203..4558)
                     /locus_tag="PK710_12800"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT10648.1"
                     /translation="MSDADFIYGLLAPGTWVEERYQIGELIGKGGSGLIFQALDTTNG
                     QPVALKTLNADLLSHPSAVTRYHREAKAAGSIGHPNICTVSDVGQLPDGRPFLVMELL
                     TGRSLADCIRDDGALPFSQVLNILTQTLSALEAAHEKKIVHRDIKPDNVFLTDRIQGI
                     TAVKLLDFGIAKSLDENAELSLTRTGMVVGTPFYMAPEQARGEKLDHRVDLYACGVLL
                     YEMLTGHRPYHATNYNALMVQVLSSPPPDPRHIRPAIPAGFAAAVQRAMAKDRRERYP
                     NARSFLAALWELRAAIAEGPRPEEVERIAQAIQSPAHRPFESNDSLEIPVFHEAEPQP
                     PISLGMVENIDSETVTQPFVRPEQLRFKRPASRSSFSDVTEVMMDAPWLEHHPPSEPP
                     APLSSSSQVAPSSWDDEPTRVDNELPSTPKSGTASTTPKPQPSKVPRPAIRAPRPKPK
                     S"
     gene            4796..5293
                     /gene="gpt"
                     /locus_tag="PK710_12805"
     CDS             4796..5293
                     /gene="gpt"
                     /locus_tag="PK710_12805"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: protein motif:HMM:NF006613.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HOT10649.1"
                     /translation="MTHPYRQDFFVSWEELHRDTRILCQALLDQQTDWHGIVAITRGG
                     LIPAAIVARELDIRLVDTVCVVSYAGREGREQTDEVRVLKQVSGDGTGLLLIDDLVDT
                     GQTASVIRTMLPKAFFATVYAKPDGRSQVDLCVREVTQDTWIRFPWDMALDFAVPLVE
                     RVRQK"
     gene            5348..5680
                     /locus_tag="PK710_12810"
     CDS             5348..5680
                     /locus_tag="PK710_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10650.1"
                     /translation="MGTELVLCSQCGRHIRNSEETCPFCSSPRVAMHTGMLAAAVTAV
                     MMGGCGPGPVAVYGPPPQSANPPTSEQQRPSPSDQDNQGNADQDGDRPEYLEPPVAVY
                     GPPPDSDP"
     gene            5782..7464
                     /locus_tag="PK710_12815"
     CDS             5782..7464
                     /locus_tag="PK710_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10651.1"
                     /translation="MQGQDEEETVTPKRSRIQRLLAACFEAIRIRIVGFCRPRSWGQR
                     HAWMLAFFALACLSLGLIWLPPLPPFVDSPSVAYSGMVLHALQKGDPFFSSWYEARPG
                     AVSHLAFYRLFGLLLNVLSPVHAIKLTVSVVVLALPLGMRSLLRAMGRSEWLCLAGFA
                     LAFNLQLAMGYLPFLLAIPLLPLCLAWVENNAKGMRWWRVVALILTLSVSPFFHLLIT
                     PVLFACVGLWVVLSQHSFRRVAYHGAAMTVAFAAVIWLVPKPRTPLPAHVSQAMGTLS
                     LSEHLDHFDGDFLNWTINGWDALSTPLFFAAVIATISLTSVIGRPTESQSLWRRFCEY
                     RAEIMAALMFAGYMASPTSVHYPWVAWSIDTRLTVFVGMFLVMVPRAVPATMRQALRM
                     APILVFSFFHLFSLVGPFRAFAHATRGLVEVSHSIPSHSIVLPVFSHEYMQDAKRWRF
                     HGFHGFINQHLVRWSAVLSTSLQPYSFCDLPFHPLICTQQLPMPGARNPMPGLSAHAK
                     NYEYVVMLTNQPDKQREHLRLIEKQGFEKMSESGDWTLWKRKTEANQTKTEK"
     gene            complement(7597..>8226)
                     /locus_tag="PK710_12820"
     CDS             complement(7597..>8226)
                     /locus_tag="PK710_12820"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOT10652.1"
                     /translation="RDHLRTALSALQAPEMAGSFPVECAEAVASALAIVFSAAKGQDW
                     QEQDRLREASHKATERRAENNDSMVPIALSRRRGASNKAPEVLAPQAEVALERRQADR
                     VLLEADVGLVSESQFYAGLSMDLSVGGVFIATYQRKPIGAPVALSLVLPGGHLVWAKG
                     VVRWIREAAGDENVPGIGVEFTEVEPADMKAIRQFCKMRAPLLFDADDV"
BASE COUNT         1685 a         2377 c         2378 g         1786 t
ORIGIN      
        1 gtcgacgcgg aatctggtgt tgcagacggg tcggcgggaa ccggtggctc agcgggtgtt
       61 ggaggttccg cgggcgttgg gggaacgagt ggggaagcag gagcagcagg caaaggtgga
      121 gccgccggcg cgggaggcat cggtggttcc gggggagcag cgggttcggc gggaaccggg
      181 ggttccgcgg gaacagcagg cagcggaggg accgcagggg cggggggcac cgggggttcg
      241 ggcggttcag gagggaacgg gctgccctat ccaacaagaa atgcctataa aatcaagggt
      301 ttacaacccg atttctggcc aaaccccgac gaaatctccg gcaacaacac cgggggcgtc
      361 gccatgaatc tcgtatgggc ccattgggaa ccgagcaaga aagccccccc ttgcgctagc
      421 aacgaagagg agttcgacgg ccactgcttc gtcgtcaatt ccaaggtgga taaagccatt
      481 gcggattgga ccgcccgcgg actcgtcgtc acggcggtcg tatatggagt gcccgcgtgg
      541 gcgaggattt catcgggctg ctccccggta tccccggggt tcgaaatctt ctgtgcacca
      601 aaaaacgcca atgattacgg acgcttcacg ggtatgctgg cgcgtcgtta caacggtctt
      661 cacggacacg ggcgcatcgc ggacttcgtc atccacaacg aagtcaacgc caacgattgg
      721 ttcgatattg gctgcgggca aggaaagcca tgcgatccca aagcgtggat ggacacgtat
      781 gcggccaatt acaacgcggc ttacgatgcc gtgaaggccg agcaaaccga agccaagatc
      841 ttgatctcgt tggagcatca tttcggctcg agttatgatg ctccagccgc gaataacccg
      901 cttttatccg ggcagaccat gctgacgggg ctggcttcac gggttggctc ccgcgcctgg
      961 cgggtggcgt atcaccccta ccctccgaac ctcctggctc ctgcttttgg gcccgatgat
     1021 tggcctcgcg tgacgtatgg aaatctgggg acgctcgcgg gatggcttcg aaaaaccttt
     1081 cccaacacgc ccagcgcctg ggaaatccag cttacggaaa gcggcgtgaa ctccttggcg
     1141 ccgcagtcca gtcaagccgc ccaggccgat ggggtctgcc ggtcgttgcg caacgtgctc
     1201 ggaacccccg gaatcgaaag ctatatctac catcgcatga ccgatcatcc cgcggaaacg
     1261 gctgcgggac ttggcctcgg acttcacgat gacaaaggcc atgccaaaga agcctgggca
     1321 gtctgggccc tcgcgaatcg aaacgacctc aatcctccca agctatcttg cggcttcgaa
     1381 cacctcccgt acacgcgtct gacgcgctcc tatcattcca gccgcggcca ttgggcctct
     1441 tcgcgcatcg ccccgtcggg attcaaaacc gaacagtcat ggcgtctgct gcgagccccc
     1501 aaagccggaa ccaagatgct gtacgaatgc ctggtcggta gccacaatat gttgtcgacc
     1561 tccgtcaatt gcgaaaacca gcatccgatg ggccccgtgg gctatattta cgaaaccaag
     1621 cagagcggga ccgttgcgtt gcatcgctgc cgaattggag ccggttcgga tcatttcgtg
     1681 tccacggacc caggatgcga aggacagaag ttcgaacaat tgcttggata tgtgcttccg
     1741 taaggcatgg ggccagtcca ttcatcccct tgctccatgg ttcgcccaaa aatagcccag
     1801 ccttccccct ggtagcgtct gacgatgagc gtggccacga cggcccttta ggatggtttg
     1861 gcatacggat gcaggccagc cgcttgcgaa acaacggctg attcccgttt tgagcgttct
     1921 gaacgaggcg aggccaaggc cccctccacc cccgcacggg aaccaacgcc taaaaacacg
     1981 gtcgcgcctc ttcgaatcga cctcagcttc tgggtatggc ttgcccattt gctgatatgg
     2041 ttctttgcaa acatgcgcga ccccacccgt ttcgaacggc ttctgctcgg tctgccagag
     2101 gatctggcgg cacgagtgcg agccctcttg ccatcgactc atttcgaggc ggcccatgct
     2161 catcttcagc gattcgctgc ggccatgcgc attggttcgc tggccaccgg aacgtggcga
     2221 ggccaggaca tttccggcga agacatccat atccatgtcc aggccgaatc cgtcgccttc
     2281 gaagatgcca ccgtgcagct tcatgtcaaa gtcggcctcg gcttccaggt ccccagtttg
     2341 cgaaagccca aagtctacaa actcgggcct gtcgaggaaa gcgtttccac cccgaaattg
     2401 cctcgcatcg atatctcttc cctcgccgga ggcctgtcca cgacgttcac cttgtcgagc
     2461 gtggaagccg atgaagtgat cgcggacgtc gctcccattg ccaacgccaa cgcggggacc
     2521 atcgtggtgg aagacatcga cctgcaggag ttgtcgatac cgcaaggtcc ttccgcaagc
     2581 ttgcagctcg atcatgccaa ggtcgagcgc gccgttcttc gagaagcgtc ggtagccaac
     2641 tgcaaaacgg cccgcgcggg tcttgccgag cccctcacag cgtcggatgt cgtcgtgaaa
     2701 ggcgcaagga tcgtcaacgg tcgagccacc gcctccggtc aggacttgga ccttggattc
     2761 gaggtccaac tgcccgcgtt gaccatcaaa acctttcctt cgatgcccgc cgccatcgat
     2821 cgtctcgtga cgcgtctttc ggtaagaatc gaacccaagg ttgtttttca ggttggaaaa
     2881 ttgaaattgg aagggatgac gttgacgaca cgcgtgggca cgctgcgcgt gaaagccttg
     2941 tcggttccct tgcaaatgca tgaattgacg atggaagacc ttcacgtgca agaagcgaag
     3001 gccgaagggc tagagattca tacccgtgat gaggcccagg aggcggacga cgcgaagggc
     3061 ctcttacctt cccaagagga ataaatatac aataatttca actagataca ccgtagttta
     3121 tggtgttttt tccagcgcat aagaagatgg cggacgaagt ggggcagaac gaagtgctgt
     3181 ccgatgagct gtctgcctga ggctaggatt tgggcttggg acgcggggct cgaatcgctg
     3241 gtcgtgggac tttggaaggt tgcggcttgg gggtggtgga agcggtgccg cttttgggtg
     3301 tggatgggag ttcgttgtcg accctggtgg gttcatcatc ccacgaagaa ggggcgactt
     3361 gggaggagga actcaagggc gcgggcggtt cggaaggggg atggtgttcg agccagggcg
     3421 catccatcat cacttcggtg acatccgaaa agctcgagcg cgaggcaggg cgtttgaagc
     3481 ggagttgttc gggccgtacg aaaggttgcg tgacggtttc gctatcgata ttttctacca
     3541 tgcccaggga gatggggggc tggggttcgg cttcgtgaaa cacaggaatc tcgagggaat
     3601 cattggattc gaagggacga tgggcagggg attggatggc ctgcgcgatt ctttcgactt
     3661 cttcggggcg gggtccttcg gcaatggcgg ctctcaattc ccagagcgcg gcgagaaaac
     3721 tgcgtgcgtt gggatagcgt tcccgtctgt cttttgccat ggcgcgttgc acggcggcag
     3781 cgaaaccggc ggggatggcg gggcgaatat ggcgtggatc cggcggcggg ctcgacagca
     3841 cctgcaccat caacgcatta tagttcgtgg cgtgatacgg gcgatggccg gtgagcattt
     3901 cgtagagcag cacgccgcag gcgtacagat ccacgcgatg atcgagtttt tctcctcgcg
     3961 cttgttcagg cgccatataa aacggcgttc ctacgaccat ccccgtgcgt gtcagagaca
     4021 attccgcgtt ttcgtcgagg gacttggcaa ttccaaaatc cagcagcttg accgccgtaa
     4081 tcccttgaat tcgatcggtg agaaaaacgt tatcgggttt gatgtcccga tggacgattt
     4141 tcttttcgtg cgccgcttcg agagccgaca gggtttgtgt caagatattg agcacttgag
     4201 aaaacggcag cgcgccatcg tctcggatgc aatccgccag cgatcgaccc gtcagtaatt
     4261 ccatgacgag aaaaggtcgt ccatctggaa gctgtccgac atccgacacc gtgcagatat
     4321 tgggatgccc gatgcttccc gcggccttgg cttcccggtg ataacgggtc accgcggaag
     4381 gatgggacaa aaggtcggcg ttgagggttt tcaaggccac cggttgaccg ttcgtggtgt
     4441 ccagcgcttg aaaaatcagt ccacttccgc ccttgccaat gagttcacca atctggtagc
     4501 gctcttcgac ccatgttccc ggtgcaagca gcccatagat gaaatccgca tcgctcattc
     4561 agtcacgtcc ttgatccacg gtgccgcgac gaacgggatt tgacggctcc tcagggcaga
     4621 tcatcaaggc aaccatacgc aaccccgcgc gtgtcgtcta gctttctcga tagccacaac
     4681 accgaagtcc aaccgcaaac gggcagccct gctgggctcg tggaaggaaa cgtggttgca
     4741 gccccaagcc acttgagtct acccatggtt tatccgtcca agcgaggcca ccatcgtgac
     4801 gcatccgtat cgccaagact ttttcgtgtc atgggaagaa ctgcatcggg atacacgaat
     4861 cctttgccag gccctgctcg atcagcaaac cgactggcac ggcattgtgg ccatcacccg
     4921 aggcggcctg attccagcag ccatcgtggc cagagaactg gatattcgtc tggtcgatac
     4981 agtctgtgtg gtcagctacg cggggcgaga aggtcgggag caaacggatg aagtacgcgt
     5041 attaaaacag gtttccggag atgggacggg gttgttgttg atcgatgatc tggtggatac
     5101 gggtcagacg gcatcggtga tccgaaccat gcttcccaag gcgtttttcg ccacggtgta
     5161 cgccaaaccc gatggtcgat cgcaggtcga tctttgtgtt cgggaagtga cccaggatac
     5221 gtggatccgc tttccttggg atatggcgct ggattttgcg gttcctttgg tggagcgggt
     5281 gcgtcaaaaa tagccattgc atggatgaaa cggctcccac gacgggatgt cgtgatagga
     5341 tggcaggatg ggtaccgaac ttgtgttgtg ctcacaatgc ggacgccaca tacgcaacag
     5401 cgaagaaacg tgtccgtttt gttcttcccc tcgggtggcg atgcatacgg gtatgctcgc
     5461 cgcggcggtg acagccgtga tgatgggggg atgcggtccc ggtcccgtcg ctgtttacgg
     5521 ccctcctccg caatccgcga acccgccgac cagcgagcag cagcggccct ccccttccga
     5581 ccaagacaac caaggcaacg ctgaccagga tggcgatcgc cccgaatacc tagaacctcc
     5641 tgtcgcggtc tatggtcctc cacccgactc ggatccttga tttcgacggg tgaaacacaa
     5701 cggctcccga cgttttcagg gcttttccac accagccatg atgtcgtgcg caatcctccc
     5761 aacgttggat gaaggctgag catgcaaggg caagacgagg aagagacagt cacgcccaag
     5821 cgttctcgaa tccaacggct gctcgccgca tgtttcgagg ctatccgaat acggatagtt
     5881 gggttctgcc gtccgcgttc gtggggacaa cgccacgcgt ggatgcttgc gtttttcgcg
     5941 ctcgcctgtc tttcccttgg tctgatatgg ctgccccccc tgccaccgtt cgtcgattcc
     6001 ccctctgtcg cctactcggg tatggtgctg cacgcattgc aaaagggcga tcctttcttt
     6061 tcgtcgtggt acgaagcgcg tcccggtgcc gtcagtcatc tcgcgttcta tcgtctattt
     6121 gggctcctgc tcaatgtctt atcccccgta catgccatca aactgacggt cagcgttgtc
     6181 gtgctggcgc tgccccttgg catgcgctcg ttgcttcgcg cgatggggcg aagcgaatgg
     6241 ctttgccttg cgggatttgc gctggctttc aacctccaac tcgccatggg atacttgcca
     6301 tttttgctgg cgattccctt gctgcccctg tgtttggcgt gggtcgaaaa caatgcaaag
     6361 ggtatgcgat ggtggcgcgt ggtggcgttg atcctcaccc tttcggtttc gccattcttc
     6421 catctgttga tcacacccgt gctatttgct tgcgttgggc tctgggtggt gttgagtcag
     6481 cattcctttc gtcgcgtggc gtatcacggg gcagcgatga cggtggcttt cgcggccgtg
     6541 atctggctcg tcccaaaacc tcgaacccct cttcctgccc acgtttctca agccatggga
     6601 acgctctctc tcagtgagca tctcgatcat ttcgacggtg atttcctgaa ttggacaatc
     6661 aatggctggg atgcgttatc gacgccactt tttttcgctg cggtgatcgc aacgatatcc
     6721 ttgacgagcg tgattggcag accgacggaa tcgcaaagtc tgtggcgaag gttttgcgag
     6781 tatcgcgcgg aaatcatggc ggcattgatg tttgcgggat atatggcctc cccgaccagc
     6841 gttcattatc cctgggtagc atggtccatc gatacgagac tgaccgtatt cgtcggcatg
     6901 ttcctcgtga tggtgccacg cgcggtgcca gccacgatgc gtcaagctct acgtatggcc
     6961 ccaattttgg tgttttcctt ctttcatctt ttcagtttgg tgggaccttt tcgtgctttt
     7021 gcccacgcca cacgaggatt ggtcgaggtg tctcactcca taccctccca ttcgatcgta
     7081 ttgccggtat tttcccacga atatatgcaa gatgcgaaac gttggcgctt ccatggcttc
     7141 cacggcttta tcaaccagca cttggtgcga tggagcgccg tgctttccac gagcttgcaa
     7201 ccctattctt tctgcgatct gcccttccat ccgttgatat gcacgcaaca acttcccatg
     7261 ccgggagccc gtaaccccat gccagggttg agcgcccatg ccaaaaacta tgaatacgtg
     7321 gtgatgttga ccaaccagcc ggacaagcaa cgagaacacc ttcgcctgat cgaaaaacaa
     7381 gggtttgaaa aaatgagcga aagcggcgac tggaccttat ggaaacgaaa aacggaggcg
     7441 aaccaaacga aaaccgaaaa atagcgttgc gcaaaacgca agtcattcct gcagggctta
     7501 tcccacgctc ccttccaaaa gaaccgcggg cgcatgaaaa tccgatagat ccgacccacg
     7561 tttctcgagc ccctcagcca gggcaccgtc acgccctcac acgtcgtcgg catcgaataa
     7621 tagcggtgcc cgcatcttac aaaattgcct gatcgccttc atatccgcag gttccacctc
     7681 ggtgaattcc acaccgattc caggaacgtt ctcgtctcca gcggcttctc gtatccaccg
     7741 cacaacccct ttggcccaca ccaaatgccc ccctggcagc accaacgaca acgccaccgg
     7801 cgcaccaatc ggttttcgtt gatacgttgc gataaaaacc ccgccgacgc tcaaatccat
     7861 cgacaatccg gcataaaact gagactcact gaccaaaccc acatcggctt cgagcaaaac
     7921 ccgatccgct tgccgtcgtt ccagcgcaac ttccgcttgc ggtgcgagaa cttccggtgc
     7981 tttgttgctt gccccacgac gtcgcgacaa ggcaatagga accatcgagt cgttgttttc
     8041 cgctcgtcgt tcggttgctt tatgggatgc ttcgcgcagc cgatcttgct cttgccaatc
     8101 ctggcctttg gcggcggaaa atacgatcgc cagggcagaa gccaccgcct ccgcgcattc
     8161 caccggaaaa gaccccgcca tttccggcgc ttgaagcgcg gacaacgctg tccgaagatg
     8221 gtcccg
//
LOCUS       DAOSOR010000220         8191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8715_length_8191_cov_3.760939, whole genome shotgun sequence.
ACCESSION   DAOSOR010000220 DAOSOR010000000
VERSION     DAOSOR010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8191
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8715_length_8191_cov_3.760939"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..2131)
                     /locus_tag="PK710_12825"
     CDS             complement(95..2131)
                     /locus_tag="PK710_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10653.1"
                     /translation="MLSALGCANGEEAENISNAKPQDASSDTAGDVVKDGAKDVADEG
                     VEEGGPTECVPKTCAQLEAECGSAPDGCGGKIDCGECMSGQVCGGGGPNRCGTSACFP
                     KSCVQLGAQCGYISDGCATAIDCGTCAAPQTCGGGGKSNECGCTPKTCSQLGASCGVV
                     PDGCNGTLNCGDCAGGQVCGGAGPNVCGTGECTPKTCAQVGASCGYASDGCSKALDCG
                     KCDPPDQCGGSGVANQCGCTPKTCGQLGANCGPVDTGCGSADCGSCDAPDTCGGGGVP
                     NQCGCTCSLPNAVTSCLGGVCMIKSCNAGWADCDGIADNGCEANLATNKNNCGSCGKI
                     CADVHGAAVCVAGDCNILCDSGWGNCDGNAANGCETDTRSDPAHCSGCGRACSTQNFN
                     PACEAGKCTGSCHDGYADCNNDKLIDGCEANTSSDPANCGGCGIVCSSQNISVTCAQG
                     ICNGVCATGYDDCNGNKQTDGCETHLLNNLDACGSCGNSCRSPMPPNVASVACNGTTC
                     EVESCESSYVDEDHQFANGCECLSDTVSDSCSTPTLLGTIGLSTWTSPTYSIMPAGDR
                     DWFRGTLNMSVSTCTQRPRIRLLDQSGLLRMAVYDSVSCPPSSGYACGTGNSTTGIRT
                     WEFGHSATCQDMGTIDPTPVTGSYSTISTSFLIEVYSSDSAASCLPYQLEFTRY"
     gene            complement(2342..3877)
                     /locus_tag="PK710_12830"
     CDS             complement(2342..3877)
                     /locus_tag="PK710_12830"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOT10654.1"
                     /translation="MNHLRTFTGSLPILFSFGALFHCSTPDSNAEADSLTQPVVGGVP
                     AQPSDYPSTVAVTNVSGDPFCSGTLIAPSVVVTAAHCVEKRSPSSMRVVYGHTVPEQA
                     PASERRIVTQAVAHPHYNTNAPVDAYGLGPMHDIGVLILQDPIENGIVAPVLPNAQVD
                     AVLYPNRTLHIAGYGIYNTASQASGVLYKGLTPHIRHVPTELLGGRSGEPDACFGDSG
                     GPAYVVDQNTLWLVGATSRAWEHAVTPCGHATVYTLVSPYVDWIETVAGPLSGGNTDG
                     GFNGGGWDGGTEPDAPLLDGNPSCVPLNSVCHPITNEGCDTAAGEACRFDALMGTLGC
                     FPGPNTVPSGKICDQTSRFCQYGYFCGASIRCEKICCSDDDCPDGVPCTPIISLIGNI
                     GTCGPVTIPADAAVPDEASADASADVEDAQKADVQQPEEGGTLDGFADTSSESVPTIP
                     EASSDGELDTSEQKTDADFIPPDAIQPGCACAQHPSGALRSTGLLWLGACVFGWIRRR
                     RRS"
     gene            complement(3881..4744)
                     /locus_tag="PK710_12835"
     CDS             complement(3881..4744)
                     /locus_tag="PK710_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10655.1"
                     /translation="MNRCAVVLSPHLDDALLSCPGWILERSRNGCIPVVAVPFSERGH
                     GNKEMHCAESAMATLGALVRSGGFAHAAARGALFNSYSKQLCQPMDLDTVGHVMAWFH
                     ALIDELTPSEVLAPLGVDQHVDHRISHQIAMDLIDKYPRISFSFFEERPAVYVQEAIW
                     LRLAHLNMASHEFESLPMTVRCERFFQSYFGHPATRLQVRPGDPSPIVVHWLEVFARG
                     TQSPMATIHGICSSWPLDLLSDWLVALDSLPSTSCGFIGSPLYVQWAAVEYAKQLGFL
                     GSYVERSWKLG"
     gene            5004..7061
                     /locus_tag="PK710_12840"
     CDS             5004..7061
                     /locus_tag="PK710_12840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT10656.1"
                     /translation="MAKKALIIDVEPTLTDLLGLFLRRYGFEVIATHDGLVGFERITV
                     EKPDLVVMDVLLPRIRGHELCWRMKQDPSLATIPVIVMSSIYEGYAYMDQAQKAGADA
                     FMLKPIEPRAFVEKVAKLVGPLPRPTQHDKPLHHQLWDIRQRYEKELPHKIAQIEAGY
                     RKAVGDANRRLLAEVHMATHSVVGTAGSLGLHDYTALAKPFELHVVRAMGADGPFTQE
                     EEREGIELLYALRALFSGEVQHVSQAPAPAPAPKIDVSSAPPPSSSLITPRAKTVAVI
                     EHDAELAQNLHRVLSQFGYAPRVFPMTIDAIPMVANEDVKALVVDMSMPIRRGGGVEF
                     VTELRNATNRVPPVVFLAPKEDWDTRARAVRAGGDAFIVKPVDTDRLIDALGGLTTCN
                     DAGPNRALIIADVARTADHLAHVLRTEGMLAQGITNPSHTLCAIAALLPDVIVVDIRG
                     DQQAAVEMVQVLRQHHHYSHIPFVMLSLAPAFQAQVRLVGWGPDDFVDRAKGSEYVAA
                     VVQARSRRARLLQAFTLRDALTGLPSHLRMMEELRRSVVQASRERQELCYAYADLDFT
                     GKINDEYGIAAGDQMIRDFGALLRERLRRTDIVGRLGSDGFGILLPNTKAWAAARVLD
                     EIRMAFGARHFRGTTGFIPSVSVGVAEYPRCEDAGSLHSAVERALFAAKESGRNRVEI
                     GTC"
     gene            complement(7097..>8191)
                     /locus_tag="PK710_12845"
     CDS             complement(7097..>8191)
                     /locus_tag="PK710_12845"
                     /inference="COORDINATES: protein motif:HMM:NF015910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOT10657.1"
                     /translation="LVMPTRRVKLVMLAVAAVLLWLAIVRKFEPLLLVPIGFGAMMVN
                     LPVNGLMTPPHGEEVGGLFYYLSYGIRLEIFPPLIFLGLGALTDFGPLLQNPKTLLLG
                     GAAQFGVVATFIGARMLGFTGPEAAAVGIIGGADGPTSIYLSAKLAPHLMGPIAVSAY
                     SYMALVPVIQPPIMRALTTKKERAIRMEDSASAPQWLRISFPIVVTVVCALLVPPSTA
                     LIGVLMFGNLLRECGVTERLSRAAQNELIDLITILLGLCVGMTMSGESFLEVRTLMIL
                     GLGCVAFAISTAGGVLVGKLMSRLPGRPLNPLIGAAGVSAVPMAARVAHNVAQEEDPN
                     NYLLMHAMGPNVAGVIGTAVAAGAFLALLK"
BASE COUNT         1896 a         2312 c         2316 g         1667 t
ORIGIN      
        1 agctcatggc cgactggctg atggcccacg gcaagatgga actcatggag atgtttggcg
       61 cgtatttgtg acgcattctt cgtgtgttga gcaattagta gcgcgtgaat tcgagctggt
      121 agggcaagca cgaggcagcg gaatccgacg aatacacctc gatcaaaaat gaggttgaga
      181 tcgtggagta cgatcctgtc acgggcgtgg gatcgatggt tcccatatct tggcatgttg
      241 cggaatgccc gaactcccag gttcgaattc cggtggtgga gttgccagtg ccgcaagcgt
      301 acccggaaga cggcgggcat gaaacggaat catagaccgc catacgaagc agcccgctct
      361 gatccaacaa tcggatgcgc ggacgctggg tacaagtcga gacactcatg ttgagtgtcc
      421 ctcgaaacca gtcacgatca cccgccggca tgatggaata agtaggcgaa gtccaagtgg
      481 ataggccgat ggtgcccaac agtgtgggtg tcgaacagga atccgaaacc gtatcgctca
      541 ggcattcgca gccattggca aattgatggt cttcgtcgac ataggaagac tcacagcttt
      601 ctacctcgca agtcgtgccg ttacacgcga cggacgcgac gttgggaggc atgggagagc
      661 ggcaactatt tccgcaactt ccgcaggcat cgaggttgtt cagcaaatgg gtttcacagc
      721 catccgtttg tttgtttcca ttgcagtcat catagccagt cgcacagacg ccgttgcaga
      781 tgccctgcgc gcaagtgacg gagatattct gagaggaaca gacgatcccg caaccaccac
      841 aattcgcggg gtcgctcgag gtattggctt cgcaaccgtc gatgagcttg tcattgttgc
      901 agtccgcata tccgtcatgg cagcttccgg tacattttcc tgcttcacaa gcggggttaa
      961 agttctgggt gctgcatgcc cttccacaac cggagcaatg ggcgggatcg gagcgggtat
     1021 cggtttcgca accgtttgcg gcgtttccat cgcaattacc ccaacccgaa tcgcatagga
     1081 tattgcaatc gcccgcgaca catactgccg cgccatggac atcggcacat atttttccgc
     1141 aagagccgca gttatttttg ttggttgcga ggttggcttc gcacccgttg tcggcgatgc
     1201 catcgcaatc agcccagccc gcgttgcagg acttgatcat gcatacgcct cccaaacaag
     1261 aggtgacggc attgggcaac gagcaggtgc atccgcattg gttgggaacg ccgcctccac
     1321 cgcaggtatc cggggcatcg caggaaccac aatcggcgct accacatccg gtatcgacag
     1381 ggccgcagtt ggcgccaagt tgaccgcagg ttttaggagt acatccacac tgattggcga
     1441 caccagatcc accgcattgg tcgggaggat cgcactttcc acaatccaag gctttgctgc
     1501 aaccatccga ggcatagccg cacgaggctc cgacttgcgc gcaggttttg ggagtacact
     1561 ccccggttcc gcagacattg gggcccgctc ctccacagac ttgaccgcct gcacagtcac
     1621 cgcaattcaa ggttccgttg cagccgtcgg gcaccacacc acaacttgct ccaagctgag
     1681 agcaagtttt gggagtgcaa ccgcattcat tggattttcc gcctcctcca caggtttgcg
     1741 gtgcagcgca ggtcccgcaa tcgatggctg tcgcgcaacc atctgaaata tatccacatt
     1801 gcgcgccaag ctggacgcaa gacttgggaa aacaagcgga ggtcccacag cgattcggcc
     1861 cacccccacc acagacctgg ccactcatac actcgccaca atcaattttt cctccacagc
     1921 catccggtgc cgaaccacac tcggcttcga gttgcgcgca cgttttgggc acacattccg
     1981 tgggcccgcc ttcctctacc ccctcgtcgg ccacgtcctt ggccccatcc ttgaccacat
     2041 caccagccgt gtccgaagaa gcgtcttgcg gcttggcgtt ggatatgttc tcggcctcct
     2101 caccgttggc gcaaccgagg gcgctgagca tcaataggag gaaaaacagg ggacggatcg
     2161 gtctttttac ctcccttgca ccgacagatc ccacgagtct tcgaccgctc acccttggaa
     2221 agacggaagg cagcatacat caaagaatac gggaatcccg accaccgctc aagcgcaggt
     2281 cgccacagaa cgtgaattgg gacggatttc gagcacccca gggggtcaaa accaaacgcg
     2341 tttacgaacg tctccgtcgc cgaatccagc cgaaaacgca cgctccaagc cataggagcc
     2401 cggtcgaacg caaagcgcct gacggatgct gagcacatgc gcaccccggc tgaatggcat
     2461 cagggggaat gaagtccgca tcggttttct gctctgatgt gtcaagctcc ccatcggagg
     2521 aagcttccgg gatcgtgggc acgctttcgg acgacgtatc ggcaaatccg tcgagggttc
     2581 ccccttcttc tggctgttgg acatcagcct tttgggcgtc ttccacatca gcagaagcat
     2641 cagcagaagc ctcatcgggc acagcggcat ccgcgggtat ggtgaccgga ccacaggtcc
     2701 cgatatttcc gatcagcgaa atgatgggcg tacacggcac cccatcggga cagtcatcgt
     2761 cggagcaaca tattttttcg catcgaatcg aagcaccaca aaagtatccg tactgacaga
     2821 atcgcgaggt ttgatcgcag attttccctg aaggcaccgt gttggggccg ggaaagcatc
     2881 cgagcgttcc catcagggca tcgaagcgac aagcctcacc agccgccgta tcacaacctt
     2941 cattcgtgat ggggtgacac acactgttga gcggcacgca agaagggttg ccatcaagca
     3001 gcggagcatc cggttcggtg ccaccatccc atcctccacc attgaaaccg ccgtcggtat
     3061 tacctcccga tagtgggcca gccacggttt cgatccaatc gacataggga gaaacaagcg
     3121 tgtagacggt cgcatgacca caaggcgtca cggcgtgttc ccaggctcga gatgtagctc
     3181 ccacgagcca gagggtattt tgatcgacga cataagcggg gccaccgcta tcgccgaagc
     3241 acgcatccgg ctcacccgag cgtcctccaa gcaactccgt gggaacgtga cgtatgtgag
     3301 gggttagccc tttgtaaaga acacccgatg cttgggacgc cgtgttgtag atgccataac
     3361 cagcgatgtg taacgtccga ttgggatata acacggcatc cacttgcgcg ttgggaagca
     3421 ctggagccac gattccattt tcgatcgggt cttggagaat caacacgccg atgtcatgca
     3481 tcggtccaag gccatatgca tccacaggag cgttggtgtt gtagtgcggg tgagcgacag
     3541 cttgtgttac gattcttcgt tccgaggcgg gagcttgctc gggaacggtg tgtccataaa
     3601 ccacacgcat actcgatgga ctgcgttttt cgacgcaatg ggccgcggta accacgacgg
     3661 atggggctat cagcgtacct gagcagaagg ggtcaccgct tacgtttgtg acggctacgg
     3721 tagagggata atcggaaggt tgcgcgggga ctccccctac gaccggttgc gtcagcgaat
     3781 cggcttcggc gttcgagtcg ggagtcgaac aatgaaataa ggccccgaaa gaaaagagga
     3841 tcgggagcga accagtgaat gtgcggagat gattcatacg ttatcccagc ttccaagatc
     3901 gctccacgta ggagccaagg aatccaagtt gttttgcata ctcgacagca gcccattgga
     3961 catacaaggg cgacccgatg aatccacagg aagtggaagg caacgagtcc aacgcgacca
     4021 gccaatctga taacaggtcc aaaggccacg aggagcaaat accgtgtatg gtagccatcg
     4081 gggattgtgt tcctcgagcg aagacttcga gccagtgaac cacgatcgga ctaggatcgc
     4141 caggacgaac ctgcagcctc gtggcgggat gtccgaaata ggattggaaa aaacgctcac
     4201 aacgaaccgt catcggtagg ctttcgaatt cgtgagacgc catgtttaga tgcgcgagac
     4261 gcagccatat cgcctcttgg acatagacgg caggacgctc ttcgaaaaac gaaaaggata
     4321 ttcgaggata cttatcgatg aggtccatgg ctatttgatg cgagatccta tggtcgacat
     4381 gctgatcgac accgagggga gccaagactt cggaaggagt gagttcatcg atcaaagcat
     4441 ggaaccacgc catcacgtgc ccgacggtat ccaggtccat gggctggcat agttgtttgg
     4501 agtaggaatt gaatagcgca ccgcgagctg cggcatgggc gaagcctccc gaacgaacaa
     4561 gtgcgcccag agttgccatg gcggattccg cgcaatgcat ttctttgttg ccatggcctc
     4621 gctcactgaa cggcacagca accacgggaa tacaaccgtt tcgcgagcgt tctaggatcc
     4681 aaccgggaca ggaaagcagc gcgtcatcaa ggtgaggaga gagcacaacg gcacaacggt
     4741 tcatgctcgg acctgcttcc gtcctatcac gaaagaagcg gcgaatccga tgtcgcagcg
     4801 ctcgcaaagc gtcgctttgt ctccccatcc acgcgggagc ccaacccacc ggatcatgaa
     4861 aggagcaaag cggtccatgg tatttgcttg ccctcacggt ggatgaatgt ccagagccaa
     4921 gggacagtta tctcttcctc ttgcgagccg ttgggctacg atcgggcgac agtaccgacg
     4981 gcatatgggt ttacctacgg cagatggcga aaaaggcgtt gatcattgat gtcgagccaa
     5041 ccctcacgga cttgttaggt ctgtttttga ggcgctatgg tttcgaagtg atcgcgaccc
     5101 atgatgggct ggtcggattc gagcgcatca cggtagagaa gccagatttg gtggtgatgg
     5161 atgtgctgct tccacggatt cggggccacg agttgtgctg gcgaatgaag caagatccgt
     5221 ctcttgccac gatcccagtc atcgtcatga gttccatcta cgaaggctat gcctatatgg
     5281 atcaggcgca aaaagcggga gcggatgcgt ttatgctcaa gcccatcgag ccgcgagcgt
     5341 tcgtcgaaaa agtcgccaag cttgtcggtc ctttgcctcg tccgacacaa cacgacaaac
     5401 cattacacca tcaactatgg gatattcgtc agcgatacga aaaagaactt cctcacaaaa
     5461 tcgcgcaaat cgaagccggt tatcgaaaag ccgtgggaga cgccaatcgc cgacttctcg
     5521 ccgaagtgca tatggcaacc catagcgtgg tgggcacggc cgggtctctt gggcttcatg
     5581 attacacagc ccttgccaag ccttttgaac tgcacgtggt tcgcgccatg ggagcggatg
     5641 ggcctttcac gcaagaagaa gaacgagaag gcatcgaact gctttacgcc ttacgtgccc
     5701 tgttctctgg cgaagtccaa catgtcagcc aagctcctgc ccccgcaccg gctcccaaga
     5761 tcgacgtttc ttccgctcca cccccttctt cgtccttgat caccccccga gccaaaaccg
     5821 tcgccgtgat cgaacacgat gcagaactcg cacaaaacct ccatcgtgtt ctcagccaat
     5881 ttggttacgc gccacgtgtt tttcccatga ccatagacgc tattcctatg gtggccaacg
     5941 aggatgtcaa agcgctggtc gtcgatatga gcatgccgat tcgacgtgga ggcggcgttg
     6001 aattcgttac cgagcttcga aacgcaacaa atcgggttcc tcctgtcgtc tttctcgctc
     6061 ccaaggaaga ttgggacaca cgtgcccggg ctgttcgtgc gggaggtgac gcattcatcg
     6121 tcaaacccgt ggacaccgat cgcctcatcg atgcccttgg cggcttgacc acgtgcaacg
     6181 acgcgggacc caaccgtgca ttgattatcg cggatgttgc gcggacagca gaccatcttg
     6241 cgcatgtgtt gcgcacggaa ggcatgctgg ctcaaggcat cacgaatcca tcccatacgc
     6301 tatgcgccat tgcagcgctc cttcccgatg tgatcgtggt cgatattcgc ggcgatcaac
     6361 aagcggcggt cgaaatggtt caagtgcttc gtcagcacca tcattactcg cacattccct
     6421 tcgtgatgct ttcgttggcc cccgcatttc aagcgcaggt tcgcctggtc ggttgggggc
     6481 cggatgattt cgtcgatcgc gcaaaaggtt cggaatacgt tgccgccgtc gtacaggctc
     6541 gatcgcgtcg agcacgcctg cttcaagcgt tcacgcttcg agatgcgctg acgggattgc
     6601 cgagccatct gcgaatgatg gaggaacttc gtcgcagcgt ggtgcaagcg tctcgggagc
     6661 ggcaagagct ttgttatgct tatgcggatc tggatttcac gggcaagatc aatgacgagt
     6721 atggaatcgc ggctggtgat cagatgatcc gtgactttgg agcgttgctt cgggaacggc
     6781 tgcgaagaac ggatatcgtg gggcgccttg gcagcgatgg ttttgggata ttgcttccca
     6841 acacaaaagc gtgggcagcg gcacgagttc tcgatgagat ccgaatggct tttggagcgc
     6901 ggcattttcg cggcaccacg ggtttcattc cttcggtaag cgtaggcgtg gcggagtatc
     6961 cgcgatgtga agacgcgggt tctttgcaca gcgcggtgga acgagcgctg tttgcggcca
     7021 aggaatcagg gaggaatcgg gtcgagatag gaacctgctg acggaagctt ggcaggcgca
     7081 atggggtcga taacgcttac ttgagcaggg ccaaaaatgc gcccgcggcg acggctgttc
     7141 caatgactcc cgccacgtta ggccccatgg catgcatgag caagtagttg ttggggtctt
     7201 cctcttgagc cacgttatga gccacacggg cagccatggg gactgcggat acacccgcag
     7261 cgccgatcaa tggattgaga ggacgacctg gcaaccggct catcaacttt ccgaccaaca
     7321 cccctcccgc cgtggagatg gcgaaagcaa cacatccgag cccaagaatc atcaacgtgc
     7381 gcacttccaa aaagctctcg ccactcatcg tcatacccac gcaaaggcca agcaagatcg
     7441 tgatcagatc gatgagttcg ttttgtgctg cgcgggacaa gcgttccgtc accccacatt
     7501 ctcgcaatag attgccgaac atcagcactc cgatgagcgc cgtcgaaggc ggcacgagca
     7561 aagcacaaac gacggtaacc acgatcggaa acgaaatcct tagccattgg ggcgcggagg
     7621 ctgagtcttc catccgaatc gcacgctctt tttttgtcgt gagagcacgc atgatgggtg
     7681 gctgaatcac ggggaccaat gccatgtagc tataggcgga tacggcaatg ggtcccatca
     7741 agtgcggagc cagtttggcc gaaagataga tcgatgtggg accatcggca ccaccgatga
     7801 ttcccacggc tgcggcctcg gggccggtga atcccagcat gcgagcacca atgaacgtgg
     7861 ccacgacccc aaactgggcg gcccctccta gcaatagtgt tttcggattt tgcaacaaag
     7921 gcccgaagtc cgtgagcgca ccaagaccta gaaatatcag cggaggaaag atctcgagac
     7981 gaatgccgta gctgagataa taaaacagac cgcctacttc ttctccgtgg ggaggtgtca
     8041 tcaacccatt gacgggaaga ttgaccatca tcgctccgaa gccgatcggc acgaggagca
     8101 acggttcgaa ttttcggacg atagccagcc acagcaacac cgcggcgacg gcgagcatga
     8161 ccaatttcac gcgcctcgtc ggcatcacca a
//
LOCUS       DAOSOR010000221         8177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8735_length_8177_cov_4.117582, whole genome shotgun sequence.
ACCESSION   DAOSOR010000221 DAOSOR010000000
VERSION     DAOSOR010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8177
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8735_length_8177_cov_4.117582"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..379)
                     /locus_tag="PK710_12850"
     CDS             complement(185..379)
                     /locus_tag="PK710_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10658.1"
                     /translation="MKTSPRNMIGTVTTLLWLGVGVYTIMYGASQAWKAGGFLIVAVG
                     VLRGALLLRDWNKTKDRLDA"
     gene            497..1639
                     /locus_tag="PK710_12855"
     CDS             497..1639
                     /locus_tag="PK710_12855"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT10659.1"
                     /translation="MKSPFEIGAIRPPSEAASLLVRVTRNCPWNRCAFCPVYHNAKFS
                     IRGVDDVKQDIREMAAIARMIQDVSVSLGFEGEVTWEVRKALAARKDLVPGTAQVTSF
                     LAQGAKHAFLQDANSIVMPTEDLLEVLAFLRETFPTIERITSYARAHSIVRKKSEDLK
                     AICEAGLNRVHVGLESGSDRVLTLVRKGVSAEQHIEAGLRAKQAGMELSEYYMPGLGG
                     RSLWREHAIETARVLNAIDPHFIRLRTTAIVPGTVLEDMEKLGQWEPMDDEQVIEELE
                     LLLEHLDVSSELSSDHILNLLAELQGKLPEIKPRLISTIHRFRSMSPNHRRAFILARR
                     GGMVERLDEMQDEAAQESAMLLLESVENHYGGDLPAAVRDLMAHFV"
     gene            complement(1676..2278)
                     /locus_tag="PK710_12860"
     CDS             complement(1676..2278)
                     /locus_tag="PK710_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10660.1"
                     /translation="MMKPISNPLRWLVGMGGCVLALGCKTPREAEPPIAEAAHDLAPS
                     SATESSTVDTSPPAAVSSAQQPAMAPSTEPSATPEPTPEPLPAVDVKNIGMHIGGGPN
                     DRESKQPIGDSVKPHFDALRACWKHVAEPDKPGDFGVDLLIPRQGGKAEVSHPRTAIQ
                     GEGFSDCVVGVFASIDYLKPKTGKTMVSYSVRFTPRSSDR"
     gene            complement(2275..3078)
                     /gene="thiD"
                     /locus_tag="PK710_12865"
     CDS             complement(2275..3078)
                     /gene="thiD"
                     /locus_tag="PK710_12865"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOT10661.1"
                     /translation="MTPIALTIAGSDSGGGAGIQADIKTFSALGVYGCSVITALTAQN
                     TRGVRGVFGVDPGFVRAQMDAVFDDISVSAVKIGMLGTAAIIRAVAEGLERWQPRWIV
                     LDPVMVAKGGDRLLDVDALTALRERLIPLASLITPNLPEAADILGSPMAEDLDSMVST
                     ARALQNLGARAVLLKGGHLGGNASPDFFAWDAEEQWLESRRHATQNTHGTGCTLSSAI
                     TASLALGMPLDAAVSKAKQYIDAAIAAADSLRIGHGHGPVHHFFKQWSS"
     gene            3367..3600
                     /locus_tag="PK710_12870"
     CDS             3367..3600
                     /locus_tag="PK710_12870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT10662.1"
                     /translation="MPVYEFICDECQHEFEELVFSMDEPVSCPHCGSGGANKVLSRFA
                     FKCGSTFRSSSSKGDSCCGCHPGPSGCAGCRHG"
     gene            complement(3652..5424)
                     /locus_tag="PK710_12875"
     CDS             complement(3652..5424)
                     /locus_tag="PK710_12875"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HOT10663.1"
                     /translation="MALVPAACSAGSGDQNLGSGGGGSSETDADPPEDSGKSDVGEAS
                     ATDVVEDKTCALTCSEDYRAVVGCDDEIEQLCAFDQMCAKGECLPACEAADQVRSSVG
                     CEYFPVVMDGYANAINGCFAVFVANTWPTAAHLTVDFHGSSLPIEQFGYLPKGSGTKI
                     TYEPIEPSEGIPPGQVAILFLAGPGNNPAGAVECPESPAIKNGSAQIKGTGKGFAFHL
                     ISDVPVVAYQILPYGGGNAAVTGASLLLPTGVWDTNYVAANAFRPATMGTEPSLNIVA
                     MKDGTEVRILPNVDVQGGGGVASTKANEVLTIQLSRGQQVQITQEKELTGSPIESNHP
                     IALMAGHECMQLPVDKDYCDHAEQQIPPVRALGSEYVAAPYRPRVGASEAVGYRIIGA
                     VNGTQLSFDPASVHPAKTLQMGEVAELSATQPFVVRSQGATHPFLIAAYMSGSKAVKD
                     GYGDPDFVRLVPPKQYLQRYVFFTDPTYPETNLVVVREKGAKGFSDVELPCAGKLDGW
                     EPVGTEGRYEFTRIDLVRHDFQPQGGCNNGRHEMKSEAPFGLTVWGWGTTETTSFTRN
                     VSYGYPAGESVVQLNDIYVPSVPK"
     gene            complement(5564..5809)
                     /locus_tag="PK710_12880"
     CDS             complement(5564..5809)
                     /locus_tag="PK710_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10664.1"
                     /translation="MSDVLLVIVVAAAFALMMTAQVAILAGLVRRKQWWRALSGLLMP
                     PLSLYWAHREGMRVRGIVWLAASIVYVAMVALAIWRG"
     gene            5978..7852
                     /locus_tag="PK710_12885"
     CDS             5978..7852
                     /locus_tag="PK710_12885"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HOT10665.1"
                     /translation="MSRTYSLALCVAGALALCASLPQLETAQAAAPAPPLLDVQQLKP
                     GDKGYGLTVFSGTEPERFDVEVIGVLTNFLPHQDLILVKTNHPRLQVAKVVAGMSGSP
                     VFIHGKMVGAYAYGWQFGEESVAGVTPIGAMLDELRRPIPPEAIQPLPSSPPARARKP
                     TAGIQMPAGHKFAGNPMSYSLEGHARQLAALTASPGSDHTLRPTPVVTPLMVGGLNDA
                     SLSVLSQHLSPLGLEPVQAGGAGRAEADAPSRFVDGGAIGVQLIAGDISAAGIGTVTR
                     VEGNRLVAFGHPMMNSGVSRLPTAISRIHWILASKMRSFKIGSPIRPVGSLINDRQAA
                     IVVDSTVEAPVIPLDLDIRGVQGAPHTRWTMQLAQERFMAPLLLAVALGNAIDSTTSE
                     RRDVSWHAHTTLRVRGHGEITLHDYGVAVGGTPDAGTFFRSRAVTAMGSLLSNPWQPV
                     VIDSIRSRVDIRFARDLLELRGAVPIDTEVEAGRKARIRLQLVPYEGPPEFRTVEVAI
                     PRELAGNTVEIEFTPGHQEAPPVAIPENITELLSVLPRQAYPPDSLVGGVRVGGHGVA
                     FRGQVATRLPPGALDTLRPLSSTIAPEPVPSYARTLIPLGKFVIGSTTVRVRVRDVLR
                     "
     gene            7876..>8177
                     /locus_tag="PK710_12890"
     CDS             7876..>8177
                     /locus_tag="PK710_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10666.1"
                     /translation="MNRTSVLARLVALITASFVMAQTSEASAVGTRTFRLDTLEELKG
                     GELEGISVDSLGRVRAGFVLGNLPVGDASAVWCSLVMPDGSVLLGTGNDGKVFRVH"
BASE COUNT         1649 a         2556 c         2233 g         1739 t
ORIGIN      
        1 tacagcaagc gccaaagaag atatccgccg agacaatgaa aacgatcgaa aaggtggctg
       61 ctcttgctcg ggcggatctc atgcaccagc gcaaacgtcc catgacgatc cgcagcgtgg
      121 tgccacgcga cgttcgcatc gctccgtgga ttcgatgaaa ccaatctcgt ccgcgccctt
      181 cggttcaagc atcgagccga tccttcgtct tgttccaatc tcgaagcagt aacgctcctc
      241 gcaagactcc cacggctacg atcaaaaatc cgcccgcttt ccacgcctga ctggcaccat
      301 acatgatcgt gtacacaccc acaccgagcc aaagcaacgt ggtcaccgtt ccgatcatgt
      361 tcctcgggct tgttttcatg aatcaaaccg tttgccacga tcggcccttc gccgccacaa
      421 tcatctgtca tgacgcatct tatcggtgtc ggggggattg cgtatgacgg tgctcgacgc
      481 cagatgggac gagaagatga aatcaccttt cgagattgga gcgattcgtc ctccctccga
      541 agcggccagc ttgctcgttc gtgtcacgcg aaactgtcct tggaaccgct gcgccttctg
      601 tccggtttat cacaacgcga agttttcgat tcgcggtgtc gatgatgtca aacaagacat
      661 tcgtgagatg gccgcgatcg caaggatgat ccaagacgtc tccgtatcgt tgggcttcga
      721 gggcgaggtg acctgggaag tgcgcaaggc gcttgccgct cgcaaagacc tggttccagg
      781 cacggcgcag gtaacgtctt ttttggcgca aggagcaaaa cacgcgtttt tgcaagatgc
      841 caacagcatc gtcatgccta ccgaagatct gctcgaagtc ttggcgttct tgcgcgaaac
      901 ctttcccacc atcgaacgca tcaccagcta cgcgcgggct cattcgatcg tccgtaaaaa
      961 atccgaggac ctgaaagcca tctgcgaagc tggtctcaat cgtgtccatg ttgggctcga
     1021 atccggaagc gaccgtgttc tgaccttggt tcgcaaaggt gtctcggccg aacagcatat
     1081 cgaagctggt ttgcgggcaa aacaagccgg tatggaactg tcggaatact atatgccagg
     1141 gctcggcggc cgctcgttgt ggcgcgaaca cgccatcgaa accgcccgcg tcctcaatgc
     1201 catcgatccg cacttcatcc gattgcgaac gacggccatc gttcccggaa ccgtgctcga
     1261 agacatggaa aagcttggtc aatgggaacc gatggacgat gaacaagtca tcgaggaact
     1321 cgagctattg ctcgaacacc tcgatgtttc ttccgaactc agcagcgatc atatcctgaa
     1381 cctgctcgcc gaattgcaag gtaagctgcc tgaaatcaaa cctcgcctga tctccaccat
     1441 tcatcgattc cgcagcatgt cccccaatca tcgccgcgct ttcatcttgg caagacgggg
     1501 cggcatggtc gaacgtctcg acgagatgca agacgaagcc gcgcaggaaa gtgcgatgct
     1561 gctgctcgaa agcgtggaaa accactacgg tggtgacctg cccgccgcgg tcagggacct
     1621 gatggcgcat ttcgtatgat cgatggcgca ggcttggcgt atcgggaaaa aagcttcagc
     1681 gatccgatga tcgcggcgtg aaccgtacgg aataactcac catcgtcttg ccggttttgg
     1741 gcttcaagta atcgatactc gcaaacacgc ccaccacgca gtcgctgaag ccttcccctt
     1801 ggatggccgt tcgcggatgg gagacttccg ccttgccgcc ctgccgtgga atgagcaagt
     1861 cgacgccaaa atcccccggt ttgtctggtt ccgccacgtg cttccaacaa gcacgcagcg
     1921 catcgaaatg cggttttacc gaatcgccta tcggttgttt gctctctcga tcgttgggac
     1981 ccccgccgat gtgcatgcca atgtttttca cgtccacggc cggtagtggc tcgggcgtgg
     2041 gttcaggcgt ggccgacggt tcggtggatg gtgccattgc gggctgttgc gcggaagaaa
     2101 ccgccgctgg cggagatgtg tcgaccgtcg atgattcggt cgctgaggag ggggcgaggt
     2161 cgtgcgcggc ttccgcgatc ggtggttctg cttcccgggg tgttttgcat cccaaggcca
     2221 ggacacaacc tcccatcccc acgagccacc ggagagggtt cgaaataggc ttcatcacga
     2281 tgaccattgc ttgaaaaaat gatgcaccgg tccgtgccca tggccgatgc gcaggctgtc
     2341 ggccgcggca atcgctgcat cgatatattg cttcgccttc gatacggctg catcaagtgg
     2401 cattcccaaa gccagcgatg cggtgatggc ggaagacaat gtgcatcccg tcccatgggt
     2461 gttttgggtc gcgtggcgac ggctctcgag ccattgctct tcggcatccc aagcaaaaaa
     2521 atccgggctc gcgtttcccc ccaaatgccc tcctttgagc aataccgcgc gggctccaag
     2581 attctggagg gctcgtgccg tgctcaccat cgaatcgaga tcctcggcca tgggtgaacc
     2641 caggatatct gccgcttccg gcaagttcgg ggtgatgagc gaagccaggg gaatgagacg
     2701 ttctcgcagg gccgtgagcg catctacatc caacaaccgg tcgccccctt tggctaccat
     2761 caccggatcg agcacgatcc atctcggttg ccatcgttcc aacccttccg ccaccgcgcg
     2821 gatgatggcg gcggtgccca gcatgccgat cttcaccgcg gatacggata tgtcgtcgaa
     2881 taccgcatcc atttgtgctc ggacgaaccc tgggtccacc ccgaaaaccc cgcgcacccc
     2941 ccgcgtattt tgcgcggtca gcgcggtgat gaccgagcaa ccatataccc cgagcgcgga
     3001 aaatgtcttg atatccgcct ggattcccgc gccgccgccg gaatcggaac cggcaatcgt
     3061 tagtgcaatc ggtgtcatct ctgccatcac gtgccgcgaa cacaagcgca tgcgcatcgg
     3121 gaaggcaagc gaatcgacag ggaatcatcg attcccccct gctcatccat tttggctcga
     3181 gcctttccga tcggctcgaa tccgatatcg aggtggcaat gatacgtagc ggaccgattc
     3241 acagtccggc gcttttcgtt cggtctttga acgaggctca tcatggagga gcgaccccac
     3301 cgtcctcatc tgcatcgtga cgatgcccgg cgcaggtgct accttcccgt cgcgaggaat
     3361 agcacgatgc ctgtttacga attcatttgt gatgaatgcc aacacgaatt cgaagaactc
     3421 gtgttttcga tggacgaacc cgtctcttgt ccccactgcg gttccggtgg cgcaaacaaa
     3481 gtcctgagcc gctttgcttt caagtgcggc tcgaccttcc gctcctcttc ttccaaaggc
     3541 gactcctgct gcggttgcca tcctggccca agcggctgcg cagggtgtag acatggttga
     3601 cccggtccca agcggaatcc aaaaaaatac aattatttca attagttatg cctatttcgg
     3661 caccgaaggc acgtagatgt cattgagctg aacgacgctc tcgcccgcgg gatacccata
     3721 cgacacattg cgcgtaaacg acgtcgtctc tgtcgttccc cagccccata ccgtcaggcc
     3781 gaagggagct tcggatttca tctcatgcct gccgttgttg caaccgcctt ggggttgaaa
     3841 gtcgtgtcga accaggtcga ttctcgtaaa ttcgtatcgt ccctcggtgc ccaccggctc
     3901 ccaaccgtcg agtttgcccg cgcacggcag ttccacatcg gaaaaccctt tcgctccctt
     3961 ttctcggacc acgaccagat tcgtttcggg ataggtcgga tcggtgaaaa acacataccg
     4021 ctgcaagtat tgtttgggag gcaccaatcg aacgaagtcc ggatccccgt agccgtcttt
     4081 cacggccttg gaaccggaca tatacgcggc gatgaggaac ggatgggtag ctccctggct
     4141 tcgcaccacg aacggttgcg tggcggaaag ctcggctact tcacccatct ggagcgtttt
     4201 ggcgggatgg acgctggccg gatcgaacga tagctgtgtg ccattgactg cgccgatgat
     4261 gcgatacccc acggcttccg aagcgcccac acggggccga tagggcgcgg ccacatattc
     4321 gcttccaagc gcgcgaaccg gagggatctg ctgctccgca tggtcgcaat aatccttgtc
     4381 cacgggtagc tgcatgcatt catgcccggc catcaacgca atcggatggt tgctctcgat
     4441 ggggcttccc gtaagctcct tttcctgcgt gatttgcacc tgctgtccgc ggctaagctg
     4501 gatcgtcaac acctcgttgg ccttggtgct cgccacaccg cctccgccct gcacatcgac
     4561 attcggcaaa atacgcactt ccgtgccgtc tttcatggcg acgatattga gcgatggctc
     4621 ggtgcccatg gttgctgggc gaaacgcgtt ggccgcgacg tagttggtgt cccatacgcc
     4681 cgtgggaaga agcaaagaag cgcctgttac ggccgcgtta ccgccaccgt aggggaggat
     4741 ttggtaggcc acgacgggca catcgctgat caggtgaaaa gcaaatcctt ttcccgtgcc
     4801 tttgatctgg gcagaaccgt ttttgatcgc gggagattcg gggcattcga cggctcccgc
     4861 gggattattt ccaggtcccg caagaaataa gatggcgact tgtccgggcg gtattccctc
     4921 cgagggctcg atgggctcgt aggtgatctt ggtgccgctg cctttgggca aataaccaaa
     4981 ctgctcgatg ggtagcgaag agccgtgaaa atccacggtc agatgagcgg ccgtcggcca
     5041 ggtattcgcc acgaataccg caaagcagcc gttgatcgcg ttcgcgtagc catccatgac
     5101 cacggggaag tattcgcatc ccacgctcga ccgcacttga tcggcggctt cgcaggcagg
     5161 caggcattct cccttcgcgc acatctggtc gaacgcacaa agctgctcga tctcgtcgtc
     5221 gcagcctacc accgcgcgat agtcctccga acacgtcagc gcacaggttt tatcctcgac
     5281 cacatcggtc gccgacgctt cccccacgtc cgatttgcct gaatcttccg gggggtccgc
     5341 atcggtttcc gaactgcccc cgcctccgct gccgaggttc tgatcgcctg aaccagcgga
     5401 gcacgctgcc ggaaccaatg ccatcgatgt caacaagcac aagcgaaaaa aagtggcgaa
     5461 agccatgccg tccacctcgg gaaatctcga atgaaaacac actgtagtac aagtttggcg
     5521 tttcgccatt tcgtatcctt cgcccccgca tgcacgtatc gtctcatcct cgccaaatcg
     5581 cgagcgctac catggccacg tacacgatac tggctgccaa ccacacgatc ccgcgcaccc
     5641 tcatcccttc ccgatgcgcc caatacagcg ataggggggg catgagcagg ccggacaagg
     5701 cgcgccacca ttgttttcga cgaaccagac ccgcaaggat cgcgacctgc gccgtcatca
     5761 tcaacgcaaa tgccgccgcg accacgatta ccaaaagcac atcggacatc gaaaaggctc
     5821 atcgcatcct acgacgcaag cgggttgcag gccacccttg tctcccgacc cggaacccgt
     5881 tcggccaaga gcggctttgt cggccatttg ggaggtatgc acaatccctt tcgcaatgga
     5941 cgagacagcc gcccgacttc aggtatgctc aggcaccgtg tccaggacct attctcttgc
     6001 tctatgcgtt gccggcgctc ttgctctttg tgcatcgctt ccccagctcg aaacggcgca
     6061 agccgcggct ccagcaccgc cccttctcga tgttcagcaa ctcaagcctg gcgacaaagg
     6121 atacggtctg acggtctttt cgggcaccga acccgaacgc ttcgatgtgg aagtcatcgg
     6181 tgtgctcacc aacttcctcc cccaccagga cctcattctt gtcaagacga atcatccgcg
     6241 gctgcaagtg gcgaaagtcg tcgccggcat gagcggaagc cccgtattca tccatggcaa
     6301 aatggtcggc gcctacgcct acggttggca attcggcgaa gaatccgtgg cgggcgtgac
     6361 tcccatcggg gcgatgttgg atgaattgcg acgccccatc cctccagaag ccatccaacc
     6421 gctgccatct tctccaccag cgcgagcacg aaaaccaaca gccggaatcc aaatgcccgc
     6481 tggtcataaa tttgctggaa atccaatgag ttacagcctg gagggccacg caaggcaact
     6541 ggccgccctc accgcttctc cgggttcaga ccataccctt cgtcccactc cggtagtcac
     6601 gcccctgatg gtgggcggct tgaacgacgc ttccctttcg gtgctttctc agcatctttc
     6661 tccccttggg ctcgaaccgg tgcaggcggg aggggcaggt agagccgagg ccgatgcccc
     6721 ctcgcgtttc gtcgacgggg gggccattgg tgtgcaactc atcgccggtg atatttccgc
     6781 cgcgggaatt ggaacggtca cgcgcgtgga aggcaatcgc ctcgtcgcct tcggacaccc
     6841 gatgatgaac tccggcgtct cgaggctacc taccgcgatc tcgcgtatcc actggattct
     6901 cgcatcaaaa atgcgcagct tcaaaatcgg ctcgcccatt cgtcccgtgg gttcgctcat
     6961 caatgaccgc caagccgcca tcgtggtcga ttccacggtc gaagcgcctg tgatcccgct
     7021 cgacctcgat attcgcggcg tccaaggcgc tcctcatacc cgttggacca tgcaactcgc
     7081 gcaagaacgg ttcatggctc ccttgcttct ggccgtcgcc ctcggcaatg ccatcgactc
     7141 caccacttcg gagcgtcgcg acgtttcatg gcatgcccat accaccttgc gcgtgcgagg
     7201 acatggggag ataacgttgc atgactacgg tgtcgcggtg gggggaactc ccgacgctgg
     7261 tacgttcttt cgttcacgtg cagtcaccgc gatgggaagc ttgttgagca acccctggca
     7321 acccgttgtg atcgacagta tccgctctcg agtggatatc cgtttcgctc gcgaccttct
     7381 cgaattgcgt ggcgccgtcc ctatcgatac ggaagtcgag gccggacgaa aagcgcgtat
     7441 tcgtcttcaa ctcgttcctt acgaaggccc ccccgaattc agaaccgtcg aagtggccat
     7501 tccacgggaa ctagctggaa atacagtgga aattgaattc accccgggcc accaggaagc
     7561 tcctcccgtc gccatccccg aaaatatcac cgaacttctc tccgtgctgc ctcgtcaagc
     7621 ctaccctccg gattcgctcg tcggtggcgt gcgtgttggc ggccacggag ttgccttccg
     7681 gggacaagtc gcgacgcgac tgcctcctgg cgctctggac accttgcgcc cgctctcttc
     7741 caccattgcg ccagaacccg tcccgtccta cgcccgcact ttgattcccc tcggaaagtt
     7801 cgtgatcggt tcaaccactg ttcgtgtccg cgtccgcgac gttctccgat gagtcccatg
     7861 gagcccggat tgtcaatgaa tcgcacatct gttctcgctc gtctcgtagc acttatcacc
     7921 gcttctttcg tgatggcgca aacctcggag gcaagcgccg tcggcacacg cacctttcgt
     7981 ctcgacaccc ttgaagaatt gaagggcgga gaattggaag gcatcagcgt ggattcgttg
     8041 ggaagggttc gcgcgggctt cgtgctcggc aacctgcccg tgggcgatgc ttccgccgta
     8101 tggtgctcgt tggtcatgcc cgatggttct gtgctgcttg gcacgggaaa tgacggcaaa
     8161 gtctttcgtg ttcacca
//
LOCUS       DAOSOR010000222         8176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8736_length_8176_cov_4.551041, whole genome shotgun sequence.
ACCESSION   DAOSOR010000222 DAOSOR010000000
VERSION     DAOSOR010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8176
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8736_length_8176_cov_4.551041"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2410)
                     /gene="lon"
                     /locus_tag="PK710_12895"
     CDS             complement(<1..2410)
                     /gene="lon"
                     /locus_tag="PK710_12895"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOT10667.1"
                     /translation="MFFQNDGDRPSAPSRRQPIPLLPLRDIIVFPAMVSQLFVGRDRS
                     IAALDDAMGRGKEIFLAAQKSAKTNTPAPDEIYSVGTVSVIQQMLRLPDGTVKVLVEG
                     KHRACIKQFTQIEPYFLVSIEDIVETEANGVEVEALVRSIQASFDIYVKLNKKVQPEV
                     LMTVQSIEDEGRLADTILAHLPSIKLSERQALLEMADVKSRLERLFEVMQAEIEILQV
                     ERKIRSRVKKQMEKTQKEYYLNEQMQAIQKELGGERDEFKNEIQELEDQLAAKKMSEE
                     ASSRVEKEIKKLRMMHPTSAEATVVRNYIDWILGLPWGETTEERFQLDEAEEILEEDH
                     YGLKKIKERILEYLAVQALTHKLKGPILCFVGPPGVGKTSLAKSIARATGRKYVRLSL
                     GGVRDEAEIRGHRRTYIGALPGKIIQGLKKAGSNNPLFLLDEVDKMSTDFRGDPAAAL
                     LEVLDAEQNHAFNDHYLDLDYDLSDVMFITTANTLKGIPIPLQDRMEIIELGGYTEFE
                     KLHIAQRYLLPRQRKDCGLDNVDMHLTEGAIRTIIHHYTKEAGVRALEREIANICRKI
                     ARKVVKEGPDKRHEIAARAVPLFLGVPKFRLSKAEEQNEIGLTNGLAVTSYGGDLLSC
                     EVTVLTGKGKLLITGLLEKGMEESAQAAMSYVRSRAEAFGLDPDFYQKVDVHVHFPEF
                     VPKDGPSAGVTMATSLASALMKVPVRNNLAMTGEITLRGRVLPIGGLKDKLLAAHRAR
                     LDTVIIPKENRKDLREIPRRVLRSMRIVLASHMDDVLREALMVDHPIAIFGPGSDVIE
                     YRNGE"
     gene            complement(2458..3714)
                     /gene="clpX"
                     /locus_tag="PK710_12900"
     CDS             complement(2458..3714)
                     /gene="clpX"
                     /locus_tag="PK710_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOT10668.1"
                     /translation="MDRRRDDRMNGNLSCSFCGKSQKEVKKLIAGPTVYICDECIGLC
                     NDIINEEVEKDESAAGAAPTPKPSEIKTVLDQYVIGQERAKKILSVAVHNHYKRIEAR
                     HGSDDVELQKSNILLLGPTGCGKTLLAQTLAKLLNVPFAIADATTLTEAGYVGEDVEN
                     IIVQLLQNADHDVERAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKVIEGT
                     VANVPPKGGRKHPQQDFLQVDTTNILFICGGAFVGLDGIIQRRIGQNTLGFGADIHSR
                     KDLKLGELFEQVQPEDLLKFGLIPEFIGRLPVIATLHELDEAALVDILTKPRNSLQRQ
                     YQKLFDIDGIKLKFTKGALTAVAREALERKSGARGLRAILEAAMLDIMYEIPSKVGVK
                     EVIINEDVVTKHEPPLIVYQKEAELA"
     gene            complement(3853..4503)
                     /locus_tag="PK710_12905"
     CDS             complement(3853..4503)
                     /locus_tag="PK710_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOT10669.1"
                     /translation="MSQRPSTRSQAIATIERARDFIPYPQVVETTHRGERSWDIFSRL
                     LKDRIVFLGTEINDDVANIIIAQLLFLESEDPDKDVQLYINSPGGVVTAGLAIFDTMQ
                     YVRCPVSTICIGQAASMGAVLLCAGGKGRRFALPHSRVLLHQPMGGARGQASDIEIQA
                     REIRHLKDVLIQILVDATGKTAEQIAKDIDRDFYLGAEQAKEYGIVDEVFQPKKKA"
     gene            complement(4660..5955)
                     /gene="tig"
                     /locus_tag="PK710_12910"
     CDS             complement(4660..5955)
                     /gene="tig"
                     /locus_tag="PK710_12910"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOT10670.1"
                     /translation="MMQVTVERLSPVLIEFHVTVPVERVKASMKRALDNLARSAHIKG
                     FRKGKAPQEVLQHLFGERIAADVSNRLVEDTLNAALKDKNIRPISSPQIEKPKPNPSE
                     DFAYTARFEVTPDIEKVDYEGLEVKRTAYPVSEAMIDREIEKLRLRYANLVVPEPARP
                     AQKQDVVTFDFVLEVGGKQVQDGRGVDTTAEVGSANLLPELSEAFEGKEAGASFDVAL
                     TFPEAYQRQEFRGMKGVFHITLKEIKQRVLPELDDEFAKDVGSYDTLDALRQDVRQKI
                     EKANAERAENEVAEALVVELCKRNPVPVPPSLVDQQARMQENEILERARTQGNNVQSV
                     SPDMRQRIRMDAEIKVRAGLLMAEIAKAQKLQITDDDIQKAYVELAEQTGQNVNKIKV
                     QYRDPKQREMLIGMILEDKVLTIIEQAAKIEQVTGPEAE"
     gene            complement(6188..6352)
                     /locus_tag="PK710_12915"
     CDS             complement(6188..6352)
                     /locus_tag="PK710_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10671.1"
                     /translation="MARSYSSWAEFEREEIRPSFKIGFSIEDLEDHAFERDRYDDIEG
                     DDFLDPDDIY"
     gene            complement(6396..6587)
                     /locus_tag="PK710_12920"
     CDS             complement(6396..6587)
                     /locus_tag="PK710_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10672.1"
                     /translation="MVGIKRTTKMSAALEEACLAPGAERAMVTVFWMTSRHKVRSQVL
                     SLDGMRKGRAVGLMTGKGM"
     gene            6753..7529
                     /locus_tag="PK710_12925"
     CDS             6753..7529
                     /locus_tag="PK710_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT10673.1"
                     /translation="MTTVEHKANVIAIANQKGGVGKTTTAVNLAASVAVAERRTLLID
                     CDPQANASSGVGVTPRSLEVSLYDTLIGRASLRDILRPTAVEHLWVAPATQDLAAAEV
                     ELVDEPDRAMRLKQALEAVLDDYDYIFIDCPPALSLLTINALVAADKVIVPMQCEYYP
                     LEGLTYLMSTIDRIRGALNPSLKLEGILLTMYDPRNLLARQVAMEIRSHFHVFESIIP
                     RNVRLSEAPSHGTPALLYDARAKGAQSYLSLARELLGDEA"
     gene            7526..>8176
                     /locus_tag="PK710_12930"
     CDS             7526..>8176
                     /locus_tag="PK710_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOT10674.1"
                     /translation="MSSEKKRALGRGLDALLPTVSTISSSSGASYGDRSVFTCPIETL
                     HPQQGQPRQRMDGSKLDELAASLKEHGLIEPLVVRRSPSSPDRFEIIAGERRWRAAQR
                     AGLKELLVVVKDVSPNTAFELALIENLQREDLDPIEIAEAIERLIKEYGYTHETVASR
                     LHKDRTTITNALRLLKLPPRVRTMVVEGKLSEGHARALLGALDDASIESLADKAVRG"
BASE COUNT         1722 a         2357 c         2177 g         1920 t
ORIGIN      
        1 gttcgccgtt cctatactcg atcacgtcgc ttccgggacc aaaaatcgca ataggatgat
       61 caaccatcag cgcttcccgc aaaacgtcgt ccatatgaga agcgagcacg attcgcatcg
      121 aacgcaagac acggcggggg atttcgcgaa gatctttccg gttttccttg ggaatgatga
      181 ccgtatccaa ccttgcccga tgagccgcca gcaatttatc cttgagccca ccaatcggca
      241 aaacgcgtcc acgcaaggtt atttcgcccg tcatcgcgag attgttgcgg acgggtactt
      301 tcatcagagc cgaagccagg ctcgtggcca tggtgactcc cgcgctcggc ccatctttgg
      361 gcacgaactc cgggaaatga acatgcacat ccaccttttg ataaaagtcc ggatcgagac
      421 caaacgcctc ggcacgagaa cgaacatagc tcatcgccgc ctgagcgctt tcttccattc
      481 ctttttcgag aaggccggtg atcagcagct tgccttttcc cgtgagaacg gtcacttcgc
      541 aagaaagcaa gtccccgcca taggaggtta cggcaagacc gttggtaaga ccgatctcgt
      601 tttgttcttc cgctttcgac agacgaaatt tgggaacgcc aaggaagaga ggcacggcgc
      661 gggccgcgat ttcatgacgt ttgtccggcc cttctttgac gacctttcta gcgatttttc
      721 ggcatatatt tgcgatctct cgttcgaggg cgcgaactcc cgcctccttg gtgtagtggt
      781 ggatgatggt gcggatggct ccttcggtga ggtgcatatc cacattgtcc agcccacaat
      841 ccttgcgttg ccgaggcagc aggtagcgtt gcgcaatatg tagtttttcg aactccgtat
      901 atcctcccaa ctcaatgatt tccatgcggt cttgcaaggg gatgggaatg cctttgaggg
      961 tgttggcggt ggtgatgaac atcacgtcgg acaggtcgta gtcgaggtcc aggtagtgat
     1021 cgttgaacgc gtggttctgc tctgcatcga gcacttcgag caaggcagcg gcgggatcac
     1081 cccgaaaatc cgtggacatt ttatccactt cgtcgagcaa aaatagcgga ttattcgagc
     1141 cggctttctt caggccttga atgatttttc cgggcaatgc accgatatac gtgcggcgat
     1201 gtccccgaat ttccgcttcg tcgcgcacgc cccccaaaga gagacgaaca tattttcggc
     1261 ccgtggctct ggcgatgctt ttggcgagac ttgtttttcc gacgccggga gggcccacga
     1321 aacagaggat ggggcctttg agtttgtgag tcagggcctg aaccgcaagg tattcgagga
     1381 tacgttcctt gattttcttg agaccataat ggtcttcctc gaggatttcc tcggcctcat
     1441 cgagctggaa tcgctcctcg gtcgtctctc cccaaggtag gcccaaaatc caatcgatgt
     1501 agttgcgaac gaccgtcgct tccgcgctgg tcggatgcat cattcgcaac tttttgattt
     1561 ctttttccac ccgcgaagag gcctcttcgc tcattttttt ggccgcgagt tggtcttcca
     1621 gttcttgaat ttcgttcttg aattcgtctc gctcgccacc caattccttc tggatggcct
     1681 gcatctgttc gttgaggtaa tactccttct gcgtcttttc catttgcttt ttgacgcgcg
     1741 atcgaatctt gcgctcgacc tgaaggattt cgatttcggc ctgcatgact tcgaacaggc
     1801 gttcgaggcg tgatttgaca tccgccatct cgagcaaggc ttgacgctcg gacagtttga
     1861 tggagggcaa atgggcgagg atggtatccg ccagccgacc ttcgtcttca atcgactgga
     1921 ccgtcatcag cacctcgggc tgcacttttt tgttgagttt gacgtagata tcgaagcttg
     1981 cctggatgga gcgcacgaga gcctcgacct ccacaccgtt ggcttcggtt tcaacgatat
     2041 cttcgatgct cacgaggaag tagggctcga tttgcgtgaa ttgcttgatg cacgcacgat
     2101 gttttccctc gaccagaacc ttgaccgtcc catcaggcaa acgcagcatc tgctgaatga
     2161 cagagacagt ccccaccgaa tagatttcgt cgggggccgg ggtgttggtt tttgcgcttt
     2221 tttgtgcagc gagaaagatc tctttgccgc gccccatcgc atcgtcgagg gccgcaatgg
     2281 accggtcccg ccccacgaaa agctgcgaga ccatagcggg aaagacgatg atatcgcgca
     2341 ggggcagcag agggatgggc tgtctccggc tgggcgcgga ggggcgatca ccgtcattct
     2401 ggaagaacat gggtcactcc tcgacacgga ggtcgaaaga aagaacaggg ggaaggctca
     2461 ggctagctca gcctccttct gatacacaat gagcggcggt tcgtgtttgg tcacgacatc
     2521 ttcgttgatg atgacttcct tcacgcccac cttggacggg atctcgtaca tgatatcgag
     2581 catggcagcc tcgaggatgg cgcgaagtcc gcgcgcgcca gactttcttt cgagggcttc
     2641 tcgggcgact gccgtcagcg cgcctttggt gaacttgagt ttgatcccat cgatatcaaa
     2701 gagtttttgg tattggcgct gcaagctatt gcgcggtttg gtcaggatgt ccacgagcgc
     2761 tgcttcatcc agttcatgca aggttgcgat gaccggcaac cgaccgatga actcgggaat
     2821 gaggccgaat ttgagcaagt cctcgggctg cacttgctcg aacaactccc cgagcttcaa
     2881 gtccttgcgg gaatgaatat ccgcgccaaa gccaagggta ttttggccga tccggcgctg
     2941 gatgatgcca tcgagcccga cgaacgctcc cccacaaatg aataggatat tggtggtatc
     3001 cacctggagg aagtcctgct gaggatgctt acgtccacct ttgggaggaa cattggccac
     3061 cgtaccttcg atgactttga gcaaagcttg ctgcacacct tcgccggaca catcgcgggt
     3121 gatgctagga ttgtcgctct ttcggctgat cttgtcgatt tcatcgatgt agacgatgcc
     3181 ccgttgtgcc cgctccacat cgtgatcggc gttttgcaaa agctgaacga tgatgttttc
     3241 cacgtcctcg ccaacataac cggcttccgt caacgtcgtg gcatccgcaa tggcaaacgg
     3301 aacgttgagc aacttggcca gggtttgtgc aagcagcgtt tttccacaac cggttggacc
     3361 cagcagaagg atattggact tctgaagttc cacgtcatcg ctgccgtggc gtgcttcgat
     3421 gcgtttgtag tggttgtgaa cggcgacaga gaggatcttt ttggcgcgtt cttgtccaat
     3481 cacgtattgg tcgagaacgg ttttgatttc agaaggcttc ggcgtgggtg cagcccctgc
     3541 ggcggattca tccttctcca cctcttcatt gatgatatcg ttacaaagcc cgatacattc
     3601 gtcgcagatg tacacggtcg ggccagcgat gagttttttc acctcttttt gggacttgcc
     3661 gcaaaaagag cagctcaagt tgccgttcat tcgatcgtca cgccgtcgat ccacgttaac
     3721 ctcgacaaat cgcgttgtac ggaagccgtc tgcgtccgct cgcgtagtct agatccccaa
     3781 gagcactgtc gcaagggaac ggcatggcga gcccatgcct ccctcaccgc cctacgttca
     3841 ccaaagcccg aatcaagcct ttttcttagg ctggaaaacc tcgtcgacga ttccgtattc
     3901 cttggcttgt tccgccccca aataaaagtc ccggtcgatg tctttggcga tttgttctgc
     3961 cgtttttccc gtagcgtcga ccagaatttg tatgaggaca tctttgagat gacggatttc
     4021 tcttgcttga atctcgatat ccgaagcctg tccccgcgct ccccccatgg gttgatgcaa
     4081 caacacccga gaatgcggaa gcgcaaatcg tcgccccttt ccacccgcgc aaagcaagac
     4141 agcgcccatg gacgccgcct ggccgatgca aatggtggat accgggcacc ggacgtactg
     4201 catggtatcg aaaatggcga ggcccgcggt cacgacccct ccagggctgt tgatataaag
     4261 ctgcacgtct ttgtcagggt cttcgctttc aagaaataga agctgtgcga tgatgatgtt
     4321 ggccacatca tcgttgattt cggtgccaag aaacacgatg cggtccttga gcagacggct
     4381 aaagatgtcc cagcttcgtt cccctctatg ggtcgtttcc accacttggg gataagggat
     4441 gaaatcgcgt gcgcgctcga tcgtggcgat ggcttgcgaa cgagtgctgg gtcgctggct
     4501 catggtacct cctcacgact taccttgcgg gagagaatcc cgcgagagac gaatccgaat
     4561 ataccgcgca agcccgccca tcgctccatg gaatgaggcg aagagcgagc caggcgataa
     4621 aaaacaaacg cacaatccag gaaaggtgac gcggtggggt cactcggcct ccgggccggt
     4681 gacctgctcg atcttggcag cctgctcgat gatggtcagg accttatctt cgagaatcat
     4741 cccaatcaac atttcccgtt gcttggggtc acgatactgc actttgattt tgttgacgtt
     4801 ttggcctgtt tgttcggcga gttcgacata ggctttctgg atgtcgtcgt cggtgatttg
     4861 cagtttttga gctttcgcga tttcggccat caacagaccg gcgcggactt tgatttcggc
     4921 gtccatccgg atgcgctggc gcatgtccgg agaaacggac tgcacgttat ttccctgggt
     4981 tcgagcacgc tccaggattt cgttttcctg catccgggcc tgttgatcga caagagaagg
     5041 cggaacgggg actggatttc gtttgcacaa ttcgacgacg agcgcttcgg ccacctcgtt
     5101 ttcggctcgc tccgcgttgg ctttttcgat tttttgccgg acgtcttggc gcaaggcatc
     5161 gagcgtatcg taagagccca catccttggc aaattcatcg tcaagctcag gcaagacacg
     5221 ctgcttgatt tccttgaggg taatgtgaaa tacgcctttc attccgcgga attcctgtcg
     5281 ttgataggct tccgggaacg tgagcgccac atcgaaactc gccccagctt ccttaccctc
     5341 gaacgcctcg ctcaattcag gcaatagatt ggcgctgccc acctctgcgg tggtgtccac
     5401 accacgccca tcctggactt gctttccccc cacttcgagc acgaaatcga acgtcacgac
     5461 gtcttgtttt tgcgctggac gcgcgggctc gggcaccacc aggttggcgt agcgcaaacg
     5521 aagtttttcg atttctcggt cgatcatggc ctcggaaacc ggatatgcgg tccgcttgac
     5581 ttccaacccc tcgtaatcga ccttttcgat gtcaggagtc acttcgaaac gagccgtata
     5641 agcaaaatcc tcggaaggat tgggcttggg cttttcgatc tgaggactgg aaatggggcg
     5701 aatgtttttg tccttcagcg ccgcattcaa cgtatcttcc acaagacggt tggagacatc
     5761 cgcggcaatc cgctcaccaa aaagatgctg cagcacctct tggggagctt tgccttttcg
     5821 aaagcccttg atgtgagccg atcgagccag gttatccaag gcacgcttca tcgacgcctt
     5881 gacccgctcg acgggaaccg taacgtggaa ctcaatgagt actggggaaa gtctctctac
     5941 cgtgacctgc atcatgggtc tcccatcgca agcgcagcga cggttatgcg ttcggctcat
     6001 gccatgacga gcgaacccgc cacgcgggcg ggtgctcgtg ccacgctacg gtcaccacgt
     6061 caagcctttc ggtcatcatt tgaggaacga aggaacaaaa tagcgagccc tggcgggagg
     6121 cgaccgccag ggctctcgtc ccggaaggac atgcaccgac tcggaatcga aaaaaaagcg
     6181 attcagttca atagatatcg tccggatcga ggaaatcgtc tccctcgata tcgtcatagc
     6241 gatcacgctc aaacgcgtgg tcttccaaat cctcaatgga aaacccgatt ttgaaactcg
     6301 gtcggatttc ttcccgctca aattctgccc acgagctgta gctcctcgcc atctccctat
     6361 tctcctcctg ccgtcctacc cctaggtgaa cagcctcaca tccctttgcc tgtcatcaac
     6421 ccaacggccc ggcctttgcg catcccgtcc aacgacaaga cctgcgaccg gactttatgc
     6481 cttgatgtca tccagaacac ggtcaccatc gcccgctccg ccccaggcgc taagcaagct
     6541 tcctccaacg ccgcgctcat tttggtggtt cgttttatac ctaccatgtc gcacctggta
     6601 ggatactgta cgcaccagac tgacactggc caagaatccc gacgacgaat tttaaaatcg
     6661 tcgaacttcg cttgatttcg cggacttgaa gcgcctatcc cacatcggcc ttgccgaacc
     6721 accgcctcga aggctaatgc cctgttcgcc ccatgacgac cgtggaacac aaagccaacg
     6781 tcatcgcgat cgccaaccaa aagggcggcg tcggcaaaac caccaccgcc gtcaacctcg
     6841 cggcaagcgt ggccgtggcc gaacgacgaa cgctactcat cgattgtgac cctcaggcca
     6901 atgcatcgag cggtgtcggt gtgactccga gaagccttga agtcagcttg tatgacacgc
     6961 tcatcggaag agcctcccta cgagacatcc tccgtcccac cgccgtcgaa cacttatggg
     7021 ttgcgcctgc cacacaggat ttggcggctg ccgaagtcga attggtcgac gagccggatc
     7081 gggccatgcg tttgaaacaa gccctcgaag ccgtgcttga cgactacgat tatattttca
     7141 tcgattgtcc gccggccctt agtttgctca ccatcaacgc cttggtggcc gccgacaagg
     7201 tcatcgtacc catgcagtgt gagtattatc cgctcgaagg gctcacgtac ttgatgtcca
     7261 cgatcgaccg gatacgaggg gcgttgaatc catcgctcaa gctcgagggc attttgctga
     7321 cgatgtacga cccgcgcaat ctacttgcac gacaagtcgc gatggagatt cgaagccact
     7381 ttcatgtttt cgaatccatc attccgcgca atgtccgact gtcggaagcc ccttcccacg
     7441 gaacccctgc tctcttatat gatgcgagag ccaaaggagc acaaagctat ctgagcctcg
     7501 cccgcgaatt gttgggggat gaagcatgag ttcggagaag aagcgcgcac tcggaagggg
     7561 gttggatgcg ctacttccca ccgtctccac catcagctcc tcatccggag caagctatgg
     7621 cgaccgctcc gtttttacat gccccatcga aacgctccat ccgcagcaag ggcagccccg
     7681 gcagcgcatg gatggctcca agctcgatga actcgccgca tctctcaaag aacacggtct
     7741 catcgaaccg ctcgtggttc gtcgaagccc gagttctccc gatcgattcg aaatcatcgc
     7801 gggcgagcga cgatggcggg cggcgcaacg cgcagggctc aaggaactgc tcgtcgtcgt
     7861 caaagacgtg tccccaaaca cggctttcga actggcgctc atcgagaacc ttcagcgcga
     7921 ggacctggat cccatcgaga tcgccgaagc catcgagcgg ctcatcaaag aatacggtta
     7981 cacgcacgaa accgtcgcca gtcgccttca taaggatcga acaaccatca cgaacgcgct
     8041 acgtctgctc aagctccccc cccgtgtccg aacgatggtc gtcgaaggca agctgtccga
     8101 aggccacgct cgcgctcttt tgggagcctt ggatgatgcg tcgatcgaat cgttggcgga
     8161 caaagccgta cgcgga
//
LOCUS       DAOSOR010000223         8169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8747_length_8169_cov_4.649988, whole genome shotgun sequence.
ACCESSION   DAOSOR010000223 DAOSOR010000000
VERSION     DAOSOR010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8169
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8747_length_8169_cov_4.649988"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PK710_12935"
     CDS             complement(<1..350)
                     /locus_tag="PK710_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10675.1"
                     /translation="MSDGNEPKDASQGNEALQRLQELRETSASARHAIGSYVSDLMDR
                     LTSLNEQETEAWEGVLESVKGWRQEQSRTIAMMQDLERRHFELENQYEKAGFELAAER
                     QRGDDLEARIRVLD"
     gene            552..2603
                     /locus_tag="PK710_12940"
     CDS             552..2603
                     /locus_tag="PK710_12940"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOT10676.1"
                     /translation="MRSIRLAFILPLLPLFCSSEPTIIQAAEKKPFNVRDLVQLERAS
                     SPVLSPDGRSVVFVLRTTDMEANKGRTDLWMMSTDGKNMRRLTTHEANDWNPRWMGPK
                     TIGFLSTRSGSSQIHALEIDGGEARQLTSLPIDIETFQSTPDGSLIVFSAAVYPECKT
                     IECTVEKDKKIKESKATGRVYDKLFFRHWDTWKDGKRNHLFALRTTGGEPVDLMKGLD
                     ADCPLLPFGGIEDYTIAPDGQNVAFSMKKPMGSQEAWSTNDDIWLVPTDGSASPKNIS
                     EANEARDSMPVYSPDGKSLAYLAMKQPGYEADRYRVVLYDLASGSQKVLTEAWDRSPD
                     SLTFGAKGARLWVTADHFGQHSIFSIDVRSGKEKLVIKDGSNSSPMETDRGVLYLRND
                     LRSPADFFVASASGGNERRLSTINQKRLESVGFGDAEPFSFQGAKGDTVYGYVVKPVG
                     FEANQKYPIAFLIHGGPQGSFGNSFHYRWNPQTYAGAGYASVMIDFHGSTGYGQAFTD
                     AIRGDWGGAPFEDLMKGLDAAVAKYPWLDGQRACALGASYGGYMINWIAGHTDRFKCL
                     VAHDGNLDERMAYFDTEELWFPEWEHQGTPWENPEGYAKHNPIDHVKNWKTPMMVVHG
                     SLDYRVVDTQGFSVFTVLQRKGIPSKLLHFPDENHWVLKPHNSILWHDEVLGWLDRWL
                     K"
     gene            complement(2711..2953)
                     /locus_tag="PK710_12945"
     CDS             complement(2711..2953)
                     /locus_tag="PK710_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10677.1"
                     /translation="MNRFMLIGLALVFSASVAGCFIVKSPPPEQQQQQQQEAPATPAT
                     TTASSAAPAASSAQPAGPAIPRIFGGSGAKDAGAAD"
     gene            3071..5596
                     /gene="hrpB"
                     /locus_tag="PK710_12950"
     CDS             3071..5596
                     /gene="hrpB"
                     /locus_tag="PK710_12950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HOT10678.1"
                     /translation="MQPFPIDSILQDLETALQIHPRAIVQAPPGSGKTTRVAPYLASR
                     ENGHVIVLEPRRLAARLAAQRVAEERNESIGKTVGYHVRFDSTIRPCTRIHYVTDGVF
                     VRQLLANPSLDGIETVVIDEFHERRLNTDWILGWLLNLQRTLRHDLKVVVMSATLDSH
                     ALTRYLEPCSQLQTQGKQFDVAISFSNTPDSRPLALRVASAVRSLAQQGLDGHLLVFL
                     PGAAEIRETQRACEPIAERYHLDTFALHGSMPSEEQDRAVAPSATPKIILSTNVAESS
                     ITIEGIAAVIDSGLARVAGHAPWSGLPTLNTLPISKASAIQRAGRAGRTRHGQCIRLF
                     SEADWMRRPDHDLPEIQRADLAEVALVLRGLGIQSSDAFPWLDPPAPNAWAAAETLLG
                     DLGALRHDGSLSETGQQLLRFPVHPRIARILLESERRGVADQTATAAALMTEKHLMTP
                     RRFSSHAHQGPHRNPSANLIDALDHFHGRSARSVAERSDHAPACDDFARRELDKVRRA
                     LLSVVHASTPAPANQEEAVACCVLTGYPDRVARKVRGRLVTLTVGGNAWVDSEEENVP
                     NFLVAIDAQQSQEGMNRRLIVRSYCAIDPWHVLDLFPDRIEEMTEYRWNESTDCVETI
                     ERWLYRGLVLDETRLARVASEQASHVLAEHVLALGPHAFAPERELTALLGRVDLLRKL
                     GLPMPALDMRVVHAVLREICEGKQCLSQVREAGLMQALQGMLSSQQRQMMELMAPKTL
                     RLPCGKELEIHYEVGQPPWAASRLQDFFGMTQSPRVAEGKVAVTLHLLAPNQRAVQVT
                     QDLESFWSLHYPKLRGQLSRRYPKHAWPEDGRKARPPAPGRLR"
     gene            5779..6276
                     /locus_tag="PK710_12955"
     CDS             5779..6276
                     /locus_tag="PK710_12955"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT10679.1"
                     /translation="MAERLEHKQSQSVSGVHLWLLLWKATKAQEAYARRSVEATGLCL
                     TDFGILEALLHKGPLPVNTLGKKILISSGSMTAAVDRLERQGLVERRDSTTDRRIRMA
                     HLTPKGEALIRPLFTVHARDMEQAFFPLAPSERAALAELLRKLASPTPRQAPRKPART
                     RPSRS"
     gene            6279..7199
                     /locus_tag="PK710_12960"
     CDS             6279..7199
                     /locus_tag="PK710_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOT10680.1"
                     /translation="MWVQTTQNHPISSASALSRPIEGIYKAPPLHWVGDGFRVAGYFS
                     SIPDALRKLDPFLLLDYHPAHTYSPTDRPRGVGVHPHRGFETVTIAFQGSVAHHDSTG
                     AGGVIGPGDVQWMTAASGILHKEYHEAGWAAQGGPFQMAQLWVNLPKAHKMDPPQYQP
                     IVANQMGVVSLPENAGEIRVIAGETFGVKGPAKTHTPISLLDVRLHAKGHLALPFPSS
                     YNVGMLVMQGEARLGETHTIVQHDFVVFEHSDDPIRIEAASDTQLLMLAGEPIREPVV
                     SYGPFVMNTEREIQQAFADFRSGKFGHLED"
     gene            7395..>8169
                     /locus_tag="PK710_12965"
     CDS             7395..>8169
                     /locus_tag="PK710_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOT10681.1"
                     /translation="MVSSRKKIKTLRISSWNVNGLRAAAGKGFLDWLMQSKNDIIGLQ
                     ETRAREDQLDAPLRSPKGYHAHFVAAQRPGYSGVGLLARKRWNSLETSLGIEDFDIEG
                     RLQIAQFGKLWVVNGYFPNGSGKERDNSRVPFKLAFYQKLFEVLEPLRANGGRVLVMG
                     DFNTAHREIDLARPKQNENTSGFLPEERREFDRWCQAGWVDTFRHFEPGAGHYTWWSQ
                     RYGVRQKNIGWRIDYILASQGAMQFVENAGIQSAVQGSDH"
BASE COUNT         1860 a         2464 c         2146 g         1699 t
ORIGIN      
        1 tgatcgagca cacgaattcg cgcttcgaga tcatcccccc gctggcgctc cgccgccaac
       61 tcgaaaccag ccttttcata ctggttttct agctcaaaat gtcgtcgctc caagtcctgc
      121 atcatcgcga tcgtccgcga ttgttcctgc cgccagccct tgacactctc gagcactccc
      181 tcccacgctt ccgtctcctg ctcgttcaac gacgtcaggc gatccatgag gtccgatacg
      241 tacgacccga tcgcatgcct cgccgatgcg ctggtttcgc gaagctcctg caatcgttga
      301 agcgcctcat tcccttggga agcatccttt ggctcatttc cgtccgacat ctggccctcc
      361 acactctctt ccctagccat gccgttggca gcaatctacc gtcaaacctc gtgcttggcg
      421 atcgaaacct gcgaacaacc acttgttccc aacaaggaaa tacaacgact tgaagtccca
      481 cgccgtattc tcgattcccg aaggcgggtt cctgattatg gtgaggacct cattccaact
      541 cggaggttac cgtgcgctcc attcgtttgg cgttcatcct acccctgcta ccgctgtttt
      601 gctcctcaga gccaacgatc atccaggccg cggagaaaaa acctttcaat gtccgcgacc
      661 tcgtgcagct cgaacgggcc tccagccccg tgctctcccc cgacggacga tccgtcgttt
      721 tcgtcctgcg aacgaccgat atggaagcga ataaaggacg cacggaccta tggatgatga
      781 gcaccgacgg caaaaacatg cggaggctca ctacccatga agccaacgac tggaaccctc
      841 gatggatggg ccccaaaacc atcggctttc tatccactcg gtcgggatcg tcacaaatcc
      901 atgctctcga aatcgatggc ggtgaagctc gacaactcac aagccttccc atcgacatcg
      961 agacattcca atccacacca gacggttcgc tcatcgtttt tagcgcagcc gtctatccgg
     1021 aatgcaaaac gatcgaatgc accgtcgaga aagacaaaaa aatcaaagaa tccaaagcca
     1081 ccggccgcgt ctatgacaag ctgtttttcc gtcattggga tacgtggaag gatggaaaac
     1141 gcaaccatct tttcgctctg cgcaccaccg gaggcgagcc agtcgacttg atgaaaggac
     1201 tcgacgccga ctgcccgttg cttcctttcg gaggaatcga agattacacc atcgcccctg
     1261 acgggcagaa cgtcgcgttt tctatgaaaa aaccaatggg cagccaggag gcctggtcga
     1321 ccaacgacga catctggctt gtccccaccg atggttccgc atcgccaaaa aatatttcag
     1381 aagccaacga agcacgagac tcaatgcccg tgtattcccc agatgggaaa agccttgctt
     1441 atcttgcgat gaaacagccg ggctacgagg cagatcgtta tcgtgtggtg ttgtacgacc
     1501 ttgcctcggg ttcgcagaaa gtgctgacgg aagcatggga tcgttccccg gattctctta
     1561 cgttcggcgc caaaggagcc cgtctttggg tcaccgccga ccacttcggc caacactcca
     1621 tcttctcgat cgatgtgcgt tcgggaaaag aaaaactcgt gatcaaagac ggcagcaaca
     1681 gttcgccgat ggaaaccgat cggggcgttt tgtatctccg caatgatttg cgttccccgg
     1741 ctgacttttt tgttgcgtca gcgtcgggag gcaacgaacg acgactatcc accatcaatc
     1801 aaaaacgttt ggaatccgta ggattcgggg atgcagaacc cttttccttc caaggagcaa
     1861 aaggagatac cgtctacgga tatgtggtca aacccgtcgg attcgaagca aaccaaaagt
     1921 atccaatcgc gtttttaatc cacggaggcc cccaaggctc cttcggaaac tcttttcatt
     1981 accgatggaa cccgcaaacc tatgcaggcg cgggctatgc ctccgtcatg atcgacttcc
     2041 atggatccac cggatacgga caggctttca ccgatgccat ccgcggcgat tggggcggcg
     2101 cacctttcga agacctcatg aaaggcctcg acgcggctgt cgcaaagtat ccttggctcg
     2161 atggtcagcg agcttgcgcc ctcggcgctt cctacggcgg ctatatgatc aattggatcg
     2221 caggccatac cgaccggttc aaatgtttgg tcgcccatga cggcaacctc gacgagcgca
     2281 tggcctactt tgacaccgaa gagctatggt tccccgaatg ggaacaccaa ggtaccccgt
     2341 gggaaaaccc cgagggatac gccaaacaca atcccatcga ccacgtcaaa aactggaaaa
     2401 ctccgatgat ggtcgtgcat ggttcgttgg attatcgcgt cgtcgatacc caaggattca
     2461 gcgtattcac cgtgcttcaa cgcaaaggca tcccctccaa actcctgcac ttccccgacg
     2521 aaaaccattg ggtactcaaa ccccataata gcattctatg gcacgatgaa gtgcttggtt
     2581 ggctggaccg atggctcaaa taagcactgc gcatatccct tccgctaacc aaatacggat
     2641 tgccatcggc aaatatataa ctacttgaaa ctacacgaaa agtttcgatc gtgtatccga
     2701 tgtcgagccg tcaatccgca gctccggcat ccttcgctcc gctcccaccg aagatccgag
     2761 gtatcgccgg cccggctggc tgcgcagagg aagctgcagg agccgccgac gatgcagtcg
     2821 tcgtggctgg ggtggccggt gcttcctgct gctgttgctg ctgctgctcc ggtggcggag
     2881 acttgacgat gaagcatcct gccaccgatg ccgagaatac cagagccaga ccgatgagca
     2941 tgaacctgtt catgatgcca tatccttgtc gctacgatgg gctccgacgt ctgaccgtaa
     3001 cccaatcctt tccccatgct gtcccttgcg gttctcgttt gtcaacgtta gctagtttcg
     3061 acaagggatc atgcaaccct tccccatcga ttccatcctg caagaccttg aaaccgcgct
     3121 acaaattcat ccgagggcca tcgtgcaagc ccccccgggt tcaggaaaaa ccacacgcgt
     3181 tgccccctat ctcgcctccc gggaaaacgg ccatgtcatc gtgctcgaac ctcgacgact
     3241 cgctgcaaga ctcgccgcgc aacgcgtcgc agaagaaaga aacgaatcca tcggaaaaac
     3301 cgtgggctac cacgtgcgct tcgactccac cattcgccct tgtacccgca ttcattacgt
     3361 caccgacggc gtcttcgttc gccaactgct cgcgaaccca tcgctcgatg gaatcgaaac
     3421 ggtcgtcatc gacgagttcc acgaaaggcg attgaacacc gattggattc tcggatggtt
     3481 gctgaacctt caacgcaccc tacgccacga cctgaaagtc gtggtgatgt cagccaccct
     3541 cgattcccac gcgctgacac gatacctcga gccctgtagc caacttcaaa cgcaaggaaa
     3601 gcaattcgac gtcgccattt ctttttcgaa tacgcccgac agccgccccc ttgctctacg
     3661 cgtggcctcc gccgtcagat ccctggccca gcagggtctc gacggtcatc tcctggtgtt
     3721 tcttcccggt gccgcggaga ttcgcgagac gcaacgggct tgtgaaccca ttgccgaacg
     3781 ctaccatctc gatactttcg ccctccacgg aagcatgcct tcggaggaac aagaccgcgc
     3841 cgtggctccc agcgccacac caaaaatcat cctatcgacg aatgtcgctg aaagttcgat
     3901 taccatcgaa ggaatcgcgg cggtcatcga cagcgggctg gctcgggtgg ccggccatgc
     3961 tccctggtca ggcctgccca cgctaaatac cctgcccatt agcaaggcat ccgcaatcca
     4021 gcgggcaggt cgcgccggac gaacgaggca tggacagtgc attcgcctgt tttccgaagc
     4081 cgattggatg cgacgtcccg accatgatct tcccgagatc caacgagccg atctcgcgga
     4141 agtagccttg gttcttcgag gattgggcat ccagtcttcc gatgcattcc cctggctcga
     4201 cccgccagcg cccaatgcat gggccgcggc ggaaaccctt ctcggggacc tcggagccct
     4261 tcgacatgat ggttcactat ccgaaacggg acaacaactc cttcgctttc ccgtccaccc
     4321 ccggatcgca agaatactgc ttgaaagcga acggcgaggt gtcgccgacc aaaccgctac
     4381 cgccgcggct ttgatgaccg aaaaacacct catgactcct cgtcgctttt cttcccacgc
     4441 tcaccaaggc ccccaccgca atccgtccgc caacctcatc gacgccctcg atcacttcca
     4501 tggccgatcg gcgcgatccg ttgcggagcg ttcggaccac gcacccgcct gcgatgactt
     4561 cgcaaggcga gaactggaca aagtacgtcg tgctctgctc tctgtcgtcc atgcatccac
     4621 tcccgccccc gcaaaccagg aagaagccgt ggcatgttgt gtgctgacgg gctatccgga
     4681 tcgggtggca agaaaagtcc gcggacgtct cgttacgctc acggtgggag gcaatgcctg
     4741 ggtcgattcg gaagaagaaa acgtgccgaa ttttcttgtg gccatcgatg cgcaacaatc
     4801 gcaggaaggc atgaatcgac gactgatcgt gcgctcctat tgtgcgatcg atccgtggca
     4861 cgtgcttgac cttttccccg accgaatcga agagatgaca gaataccgct ggaacgaatc
     4921 gacggattgt gtggaaacga ttgagcgatg gctttaccga gggctcgtcc tcgacgaaac
     4981 acggttggca cgcgtcgctt ctgaacaggc ttcccacgtg ctcgcggaac acgttctcgc
     5041 gctggggccc catgcgtttg cgccggaacg cgaattgacg gctctgcttg ggcgtgtgga
     5101 cttgttgcga aaacttggac tgccgatgcc agcactggat atgcgcgtcg ttcacgccgt
     5161 gcttcgcgaa atttgcgaag gaaaacaatg tcttagccag gttcgggaag ctgggttgat
     5221 gcaggcgttg caggggatgc tttcttcgca acagcggcaa atgatggagc ttatggcacc
     5281 gaaaaccttg cggctgccgt gcggcaaaga actcgagatt cattacgaag tcggtcaacc
     5341 cccgtgggcc gcgtcgaggc tgcaggattt ttttggaatg acgcaaagtc cccgggtagc
     5401 ggaaggaaaa gtagcggtga cactgcattt gttggcaccg aatcaaaggg cggtgcaggt
     5461 cacgcaagac ctggaaagct tttggagcct tcattatccg aaacttcggg gacagctcag
     5521 ccgacgttat ccgaaacacg cctggcccga ggatggccga aaagcgcgcc ctccagctcc
     5581 gggcagactc cggtgatgca cggtctgctt gctcgaagtt accgctagtc atcggccgac
     5641 cgctggcctt ccctgcggaa tatgcgctaa attcgctcat ttcagaactc tttccccggg
     5701 ctcgggggtc ggcacccttg acgatccacc cgacccaatt atcttgacgt aaagataatt
     5761 tcaaacaaga cgattggcat ggcagagcgg cttgaacaca agcaatctca atccgtcagc
     5821 ggcgttcacc tgtggctgct gttgtggaag gcgaccaaag cgcaggaagc ctatgcgcga
     5881 cgcagcgtgg aggccaccgg gctttgtttg acggactttg gaattttgga ggcgcttttg
     5941 cacaaagggc cgcttccggt gaataccctt ggaaaaaaga tccttatttc cagtgggtcc
     6001 atgacggcgg cggtggatcg tttggagcgg caaggactgg tcgaacgccg ggattccaca
     6061 acagaccgac gaatacgcat ggcgcaccta acacccaaag gagaggcgtt gattcgaccg
     6121 cttttcaccg ttcatgcgcg ggatatggaa caagcttttt ttcctctcgc gccgtcggaa
     6181 agggcggctt tagcggaact gttacgcaaa ctggcgtccc ccactccgcg acaagcgccc
     6241 cgcaaacctg cacgaacacg accttcaagg agctaattat gtgggtccag acaacacaga
     6301 accatcccat ttcttcggca tcggctcttt cgaggccaat cgaaggcatc tacaaagccc
     6361 ctccgcttca ttgggtgggc gacggcttcc gcgtggcggg ctatttctca tcgattcccg
     6421 atgcattgcg caagctcgac ccctttctct tgctcgatta ccatccggcc catacctaca
     6481 gccccacgga ccgtccccgc ggggttgggg tgcatcccca tcgtgggttc gagacggtga
     6541 cgatagcatt tcaaggaagc gtggcgcatc acgatagcac gggggccgga ggggttatcg
     6601 gcccgggaga tgtgcaatgg atgacggcag cttcgggaat tttgcacaaa gaataccatg
     6661 aagccgggtg ggccgcccag gggggaccct tccagatggc gcagctttgg gtaaaccttc
     6721 ccaaagcgca taaaatggat ccgccccaat atcagcccat cgtagccaac cagatggggg
     6781 tcgtatcttt gcccgaaaac gccggggaaa tccgggtcat cgctggcgaa acctttggtg
     6841 tgaaaggccc cgccaaaacc cataccccca ttagcttgct cgacgttcgt ctccacgcca
     6901 aaggccacct cgccttgcct tttccttcga gctacaacgt cgggatgctc gtcatgcaag
     6961 gcgaggcaag gctcggcgaa acccatacca tcgtccaaca tgatttcgtg gtgttcgagc
     7021 attccgacga cccaatccgc atcgaagcag ctagcgatac gcaacttctt atgcttgccg
     7081 gagaaccgat acgagaacct gtggtatcct atgggccttt cgtcatgaat acagagcggg
     7141 agattcaaca agctttcgcc gacttccgaa gcgggaaatt cggtcacctc gaggattgat
     7201 cttccccctg tccgcctcgt tcccgcgaac cgagacaaca ataagtaatt gaaatacaaa
     7261 tagttacacc attaccccac ggactgggtc gccacgaccg aacgccgcca ccgtggattc
     7321 caagcagcaa gccgcggtgg gtggatatcc aattgctcga cccgtcaata aggaaactgc
     7381 gctacggttc gcccatggtt tcgtcccgga aaaagataaa aactctgcgc atatcgtcct
     7441 ggaatgtgaa tggtttgcga gcagccgcgg gcaaaggatt cctggattgg ctgatgcaaa
     7501 gcaaaaacga tatcatcggt ttgcaagaga cccgtgcgcg cgaagatcaa ttggacgctc
     7561 ctcttcgctc gcccaaaggc tatcatgctc atttcgtggc agcgcagcgt cccggctata
     7621 gtggagtcgg gcttctagcg cgcaaacgct ggaattcgct ggaaacgtcg ctggggatcg
     7681 aggatttcga catcgagggg cgcttgcaaa tcgcccagtt tggaaaactt tgggtcgtga
     7741 acggatactt cccgaacggc agtggaaaag agcgagacaa cagccgtgtt cctttcaagc
     7801 tcgcgtttta tcaaaaacta ttcgaggtct tggagccgct gcgcgccaac gggggccggg
     7861 tgctggtaat gggcgacttc aacacggccc accgggagat cgacctcgcc agaccgaaac
     7921 aaaacgaaaa cacaagcgga tttttgccgg aagaacgtcg ggaattcgac cgatggtgcc
     7981 aggcaggatg ggtcgatact ttccgtcatt tcgagcctgg agccggtcat tatacctggt
     8041 ggagtcagcg ctacggcgtt cgacaaaaaa acatcggctg gcggatcgat tatattcttg
     8101 caagccaggg agccatgcaa tttgtggaaa atgcggggat tcagagcgcc gtccagggaa
     8161 gcgaccatt
//
LOCUS       DAOSOR010000224         8165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8758_length_8165_cov_3.841800, whole genome shotgun sequence.
ACCESSION   DAOSOR010000224 DAOSOR010000000
VERSION     DAOSOR010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8165
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8758_length_8165_cov_3.841800"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..2384)
                     /locus_tag="PK710_12970"
     CDS             complement(45..2384)
                     /locus_tag="PK710_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EB domain-containing protein"
                     /protein_id="HOT10682.1"
                     /translation="MKHEENYARFIVILALVSTACGGDDLECGPGTHPYGDRCVADVS
                     SCGDGTKLVDGQCVPLCADNEIWTGKHCVPDYQCAPGTSPVAGQCVPTCTKDEYWNGQ
                     SCSKTPRCALGTVFDENTGSCVPNELICAKGTHLDNGQCVPDTSACGDGTHLENGQCV
                     PNRLPAPDVVESTDPTGKVTFDLPDPGKSITLGGTVDTPIDLNGDSIVDADYDRFSFS
                     AQAGTWIRVHATSEGAAHPAFALVSDDTDAQGQVLYARYAVNPLGVDTEREFYLPRSG
                     NYTLVVSDYTHVTSDLFGWLALPVGGDDFTYLITVDNLGDPTPTPIASVPASKTGDSG
                     AGALAFYQMKDLKLHDARVAQHMGKPSGMMASDTFGALMLFGPKGTLVRESLSYSLTG
                     DATILFAATEPGDHLLVVDHLLSIGPLTDYELRVEQVDTVDCGAANCAAGTLAQGTSK
                     LLYWDLKEDNVFAMGAKVPDTESSSLQVQRMDAGLVPVGEKTSASKYGPAKIRTYIEK
                     DQRLYLLLTGYNGNAIASYELDVKTPTVDRLTSATTHSGLKVHEMPLDIYPSAGMGRW
                     DAAAGQVILSTSFTTAWPNWTQPLEEYLTLGFEPLPKLRDTTDPAFPNSILPPLMAYM
                     PKAGRFLYQTKDDAGGDIVGASYDTTFHALTPISLATPTAGNPVSKTNQGLIPTAGWS
                     LFGFSVPKGQTVALKVTPKGTEPLQAEAWIVTPGRHQYTSGNHSWVADKEEIGLGILA
                     RQAASAPGQEVQLLVEAPYDDTQLVFIRNIDAGDPTGRA"
     gene            complement(2401..5139)
                     /locus_tag="PK710_12975"
     CDS             complement(2401..5139)
                     /locus_tag="PK710_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10683.1"
                     /translation="MRSPLCFEWIGVVGLVFTIIGCGGDDTECGPNTVEKDGKCVSKI
                     QSCAPGTHLVSSRCVPLCTDAEIWDGSSCVPKTQCGPGTRLQADRCVSVCEPTQTWNG
                     TACETMTECAPGTSYNDITGECEPNEKACAPGSSWVDGQCVSDLACGPGTKAENGQCV
                     PEGLPVADVVESSEPDGAAEFKIPNPGKTIILGGVIDEPPGFGYPDWDTFVFDAKAGT
                     YLQLEAYSAGPLRPAFVLESKARHPDGSPRFSRYGAQGEGVVTRREVYLPFDGTYELR
                     VTDHDHLLASLFSNVGALPVGGDNYAYVVEIQNLGAPKPKKINTLPATESGDLADGSL
                     YFYSFPGAKAATVVSGRSVGVPPPAKVSDIFRALMAVNGSGQSIHERVTSQTQQDTEV
                     LVLGGSSEPMIVQDHLLAIGPHQEFLLEMDEIVPKDCTKNDCKSGSLADNEQALWKWN
                     LAEGDFLTVGAYLPNENRLMKASLLDSNLQFTMDDSWVSPWDVGVGYLYASEATSAYL
                     WLREVEGEKVNEYLVDARVVKTPAFSSGQTYKNLPLHEMPPYTLRNAGIGHFKGEAGK
                     VVFFSGFSTKPEGAWQAPQELLMTPHLEPMGPVIDTKAWNFPDGFVTPLFGYIKDDGH
                     YLHYVFDGGPPFSGGTYETRMTIRNTFPLGKPAQGAPIEVSNHNLSQGLALYTFEANA
                     KQYVDITVTPTLISGLVPDLWIFNFGRPEFAFVNYRWVGDMSSPRLGLIHRETGTSSK
                     AITAGYQSPYDGITVLLVQSSGGSGSITDVFGLKIETPPPPGNDTCAKAGPLTLDSSG
                     HTSFGWNMSSATNTITHSGCTDYPSDGPDTFFSVNLKEGETIDIEMSTDTFSASLYLF
                     TDCSQVKATCRGASEYGKPRRVTYTVPPGGGGKYIIGADSHEHAGWFDMDVKVSKH"
     gene            5418..5591
                     /locus_tag="PK710_12980"
                     /pseudo
     CDS             5418..5591
                     /locus_tag="PK710_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6188..7840
                     /locus_tag="PK710_12985"
     CDS             6188..7840
                     /locus_tag="PK710_12985"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOT10684.1"
                     /translation="MSCTDWMFGPFPLAVFGLLGMSASLSMIAGCGEDSSGPSYWVHG
                     DGGDERTAGDAGDLSGKDASDDSVSDHEAPPKPDGSYPEGDVPDSSASQDGQTEGAAT
                     GSCDGVDCSGHGTCSTSTGACECHEGFEGVACETLNGDYGKRVKIVEGLADPDVLKLT
                     EERFVLSGTSEGQATDFRFRQSSDLIHWDEFKVYRPSDIDPAHRYCWMWAPAIVQEAG
                     GIALYFSALQLGKFDGPCPPIEQTGKDVATFRAFASGDTFDFGKPEPLFPGTKGPRTY
                     TAAVCPELCGNAIRIDPAVLDGRLYYVYFAGGNNVTSIRLSDASDQLFHTGPDIKGAR
                     DPDEGIINEAPEAFSRQGRLYLFFSTADFRGPYTTRYMIGNTAADLRRSLTVAHRLTT
                     PAYGLNGKLVETHGHNSVTTWRGETFNFFHVGVFHQGNLVRRDTYRQRIVWKDDGTAV
                     NQNAVHVSWNSLGGAYVYSFDLVLRDGSTIGPCIGSGILGKATSVTYTGVCKDANDRL
                     VHKAEVGAFRIYASSGGGVYHKVGEVSYDGYSDTVSLTISPP"
BASE COUNT         1719 a         2104 c         2438 g         1904 t
ORIGIN      
        1 tcaaagaccg gcttgcttga gcgtcagttt gaccgggtga tcaatcacgc ccttccggtg
       61 ggatctcccg catcgatgtt tcggatgaaa accagttgag tatcgtcgta gggcgcttcg
      121 acaaggagtt ggacttcctg tccgggtgcg gaagctgctt gtcgtgccaa tatcccaaga
      181 ccgatttcct ctttgtctgc cacccaggaa tggtttcctg aggtatattg atggcgtcct
      241 ggtgttacga tccaggcttc cgcttgcaaa ggttccgtgc ccttcggagt gactttcaag
      301 gcgacggttt ggcctttggg gaccgagaag ccgaagagtg accagcccgc ggtggggatc
      361 aatccctggt tcgtcttgct tacgggattt ccggcggtgg gggttgcgag ggaaataggt
      421 gtgagagcgt gaaacgtggt gtcgtacgaa gcgcccacga tatctcctcc ggcgtcatct
      481 ttggtttggt acaagaaacg tccggccttg ggcatatagg ccatcaacgg cggcagtatg
      541 ctgttcggga acgccgggtc ggtcgtgtcg cggagtttgg gcaaaggttc gaatccaagg
      601 gtgagatact cttcgagggg ttgggtccaa tttggccaag ccgtggtgaa gctcgtggac
      661 aggatgacct gtccggccgc ggcatcccat cggcccattc ccgcggaagg gtagatatcc
      721 aagggcattt cgtgaacttt gagaccggaa tgggtggtag cggacgtcaa tcggtccacc
      781 gtgggtgttt tcacatccag ttcatagctg gcgatggcat tgccattgta gccggtcagc
      841 agcaggtaaa gacgctggtc tttttcgatg tacgtgcgaa tcttggcggg accgtatttg
      901 cttgcacttg ttttttctcc gacaggaacc aggcccgcgt ccatcctttg cacctgaaga
      961 gaagacgatt ccgtatcggg aaccttggct cccatcgcga aaacgttgtc ttccttcaaa
     1021 tcccaataca gtagtttgct cgtgccctgg gcgagggtgc ccgcagcgca attcgcggct
     1081 ccacaatcca ccgtatccac ctgttctacc cgcagttcat aatccgtcaa gggtccaata
     1141 ctgagcagat ggtccaccac cagcagatgg tcgcccggct cggtggccgc aaacaggatg
     1201 gtggcatcgc ccgtgagcga ataggaaaga ctttccctca ccaaagtccc cttcggaccg
     1261 aaaagcatga gtgcgccgaa cgtatcactg gccatcatgc ccgatggttt gcccatatgt
     1321 tgagccaccc gggcgtcatg gagtttcagg tccttcattt gataaaacgc cagggcgcca
     1381 gcgccagagt ctccagtttt ggacgccgga acggaagcga tgggggttgg ggttggatcg
     1441 cccaggttat ccacagtgat caagtaggtg aagtcatcgc ctccgacggg caaagcgagc
     1501 catccgaata gatcgctcgt gacatgagta taatcggaga cgacaagggt atagttaccg
     1561 gatcggggaa ggtaaaattc cctttccgta tccacgccga ggggattgac ggcatatcga
     1621 gcataaagga cttgtccttg cgcatcggta tcgtctgaaa ccagggcaaa ggccggatgc
     1681 gcggctccct cggaggtggc atggacgcgg atccaggtgc cggcctgagc ggagaaagaa
     1741 aagcggtcat aatccgcatc tacgatggaa tccccattga gatcgatggg tgtatccacg
     1801 gttcctccca aggtgatcga cttgccggga tccggcaggt cgaaggtaac tttgccggtg
     1861 ggatccgtgg actccacgac atcgggtgcg ggaagacggt tggggacgca ttgtccattt
     1921 tcgagatggg tgccgtcgcc acaggccgaa gtatccggta cgcattgccc gttatccagg
     1981 tgggtaccct tggcgcaaat cagctcgttt ggaacgcaac tacctgtatt ttcgtcgaaa
     2041 accgttccaa gggcgcagcg gggagttttc gaacagcttt ggccgttcca atactcgtct
     2101 ttggtacagg tgggaacgca ttggcccgcc acgggtgatg ttcctggagc acattggtag
     2161 tccggaacgc aatgttttcc tgtccaaatc tcgttatcgg cgcataaggg gacgcactgg
     2221 ccatcgacaa gtttggtgcc atcgccacag gaggagacgt ctgccacgca acggtctccg
     2281 taggggtggg ttccagggcc gcattccagg tcatctccgc cgcaggccgt cgacaccaag
     2341 gccaggatga caatgaatcg cgcgtagttt tcctcgtgtt tcatgttctg ctcctgtttc
     2401 tcagtgtttc gagactttga catccatgtc gaaccagccg gcatgctcat gggaatcggc
     2461 tccaatgatg tatttcccac caccaccggg aggaacggtg taggtcacgc ggcgaggttt
     2521 gccgtattcc gaggccccgc ggcaagtggc tttcacctgg gagcaatcgg tgaataagta
     2581 gagcgaagcg ctgaatgtgt cggtggacat ctcaatgtca atcgtttcgc cttctttcaa
     2641 attcacactg aaaaacgtgt cggggccatc ggaggggtag tccgtgcagc ccgaatgggt
     2701 gatggtattc gtggcgctcg acatattcca accaaaagag gtatggccgc tggaatccaa
     2761 ggtaaggggg cccgccttgg cgcaggtatc gttgcctgga ggtggaggcg tttcgatttt
     2821 caatccgaat acgtcggtga tggagcccga accgccggac gattgaacga gcaataccgt
     2881 aataccatcg taaggggatt ggtatccggc ggtgatggcc ttgctcgatg ttccggtttc
     2941 tcgatgaatc aggcccaggc gaggcgaact catgtccccg acccagcgat aattgacgaa
     3001 tgcaaattca ggacgcccga agttgaagat ccaaagatcg ggaaccaatc cggatatcaa
     3061 ggttggagtg accgtaatat ccacatactg cttggcgttg gcctcgaatg tgtagagggc
     3121 caggccttga gacaagttat gattgctcac ctcgatcgga gcgccctgcg ccggcttgcc
     3181 caaaggaaac gtatttcgta tcgtcatccg cgtttcgtag gttcctcccg aaaacggggg
     3241 gcctccatcg aaaacgtaat gaagataatg cccatcgtct ttgatataac caaatagcgg
     3301 cgtgacgaat ccatcgggaa agttccacgc cttggtatcg atgaccggtc ccatgggttc
     3361 gagatgaggt gtcatcaaca attcttgggg agcttgccag gcaccctccg gtttcgtcga
     3421 aaatccgctg aaaaacacca ccttcccagc ttcccccttg aaatgtccga tacccgcgtt
     3481 ccgcaaggtg tacggaggca tttcatgcag cggaaggttt ttataggtct ggccggacga
     3541 aaaggcgggc gttttcacca ctctggcatc tacgaggtat tcattcactt tttctccttc
     3601 gacctctcga agccatagat acgcagatgt ggcttccgaa gcgtataggt agcccacccc
     3661 gacatcccaa ggactcaccc aactgtcatc catggtgaac tgcaagttgc tatcgagcag
     3721 ggaggctttc atcaaccgat tttcattggg taaataagct ccgacggtca gaaaatcgcc
     3781 ttcggcgagg ttccacttcc acaaggcttg ctcgttatcc gcgaggcttc cggatttgca
     3841 gtcgtttttc gtgcaatcct tagggacgat ctcgtccatt tcgagcagaa actcttggtg
     3901 ggggccgatg gcaagcaagt gatcctgcac gatcatcggt tccgaggagc cgccaagcac
     3961 gagcacttcc gtgtcctgtt gggtttggga agtcacccgc tcatggatag actgaccact
     4021 tccgtttacc gccatcagag cgcgaaaaat atcactgacc ttggcgggag ggggaacgcc
     4081 tacggaacgg ccgctgacta ccgtcgcagc tttggctccg ggaaatgaat aaaagtacaa
     4141 ggagccatcg gcgagatcac cgctctccgt ggctggtaac gtattgattt ttttaggttt
     4201 tggtgctccg aggttctgaa tctccaccac ataagcgtag ttgtcgcccc ccaccggaag
     4261 cgctccgacg ttcgaaaaaa ggcttgctag caagtggtca tggtccgtca cgcgaagttc
     4321 atacgttccg tcaaagggca gatagacctc ccgccgtgtg acaacgccct cgccctgtgc
     4381 gccatatcgg gagaaacggg gcgatccatc cggatgtctc gctttggatt caaggacgaa
     4441 agcggggcgc agggggcctg cggaataggc ttcgagttgc aagtacgttc ctgctttggc
     4501 gtcaaagacg aaagtgtccc aatctggata tccgaatccc gggggctcat cgatgacacc
     4561 cccaaggatg atggttttcc ccgggttggg gattttgaat tcggccgcac cgtcgggctc
     4621 gctggactcg accacatcgg cgaccggcaa tccttccggc acgcattgtc cgttttcagc
     4681 cttggttccc ggtccgcagg cgaggtctga tacgcattgg ccgtcgaccc aggaactgcc
     4741 gggcgcgcat gccttttcgt tgggttcaca ctcgcccgtg atgtcgttgt aggatgtgcc
     4801 gggcgcgcat tcggtcatgg tctcacacgc cgttccattc caggtttgcg tgggctcgca
     4861 aacggaaacg catcggtctg cttgcagtcg ggtgccggga ccgcactggg ttttggggac
     4921 gcaggagctg ccgtcccata tttcggcgtc tgtacataag ggaacgcatc gggaactcac
     4981 gaggtgggtt ccaggtgcgc aagattggat tttggaaaca cattttccat ctttttccac
     5041 ggtgtttggg ccgcactcgg tgtcatcgcc gccacagccg atgatggtga aaacgagccc
     5101 tacgacccca atccactcga agcacaaggg tgagcgcatg atcacatcct tcccgaaaaa
     5161 gtgccgatgc atggtagcat gaaggcgctg gtcctgcggg tgtagaaatg gggtaggcga
     5221 ggcgaatacc catggcctat gattatgtcg caggccatga ttacctgaaa attcaatttt
     5281 gattatatac agggttttca agaatctgat tgagtgatga catgggcggg cgtggaggat
     5341 gaagttggca tttgccccac gacctgcttc cgtaggcgtc agtggcgccc catgaccaca
     5401 ctcggaaggg taatatgata aggggcagaa agcgccatat ccttgtcgac gggcacggtc
     5461 tggtgttgat tgtcagtgta acgccagccg atgggcaggg tcgtgatggc gctattccgt
     5521 tgattcacgg tgctcccagc gcatttccaa tgatacagat aattctggtg gatggcgcgt
     5581 acgggggata ggtcatcgac ttggccgagc caccaacggg tattgttctc gaagtgaccc
     5641 aacgcagtgc cgagatcaaa ggttttgcgt cagtgcgtaa gcacggagtc atggggcgga
     5701 gtttcggttg gatgggaggt tatcgtcgca cgaggaaggg ccacgagcgc tattgtatga
     5761 acgaagaaaa cgtgatcgag ggggtcatgt tcgcgattgg gtagcgcaga ctgcgacccg
     5821 ggcccgacgc gcgggcagcg gtgctgcgga ttcgtcgcac cgcctgattt gatttcaacg
     5881 tacgacaaaa cgaaacgcag ctcgaacgac ggagttgttg cgcaggggtg cgtcagcgat
     5941 gtctccggag cccctgtggt ggccaagtga cccaatgtat tggcacgcca aacgggaacg
     6001 tgcgcggctc tggtacgaaa gttcaggcta ttcggttaaa cagcctctca gagctgtaag
     6061 cagagccatt cgtgtctcgc tttttctctt ctttccgcaa atcagcacgc gggatttctt
     6121 tcacccgcga tggtgtagct ttcggctgtg gattgacacg ccttcgtttt ccacaaatca
     6181 tgaagaaatg agctgtaccg attggatgtt cggaccgttt ccgctcgccg tgtttggctt
     6241 gcttggcatg tccgcttcgc tgagcatgat cgcgggttgt ggtgaggatt catcgggacc
     6301 atcgtattgg gttcacggcg atgggggtga tgagaggacg gcaggtgacg cgggagatct
     6361 gagcggcaag gatgcttccg atgattccgt ttcggatcat gaggctcctc cgaagcccga
     6421 cggctcttat cccgagggag atgtgccgga ttcttctgcg agccaagatg gacaaaccga
     6481 aggggctgcc actggttcct gtgatggcgt ggattgttcg gggcatggca cctgctcgac
     6541 ttcgacggga gcttgcgagt gtcatgaagg attcgaggga gtcgcgtgtg agacgttgaa
     6601 cggcgattac ggcaaacgag tgaagattgt ggaaggtctt gcggatcccg atgtgctcaa
     6661 gctcactgaa gagcgtttcg ttctatcggg tacgtccgag gggcaagcca cggactttcg
     6721 tttccggcag tcttccgacc ttatccattg ggatgaattc aaggtatatc gtccgagcga
     6781 tatcgatcct gcccatcgat attgttggat gtgggcaccg gcgattgtgc aagaggctgg
     6841 agggattgcg ctgtactttt cggcgcttca gcttggcaaa ttcgatggtc cttgtcctcc
     6901 catcgagcag accggcaagg atgtggcgac ctttcgagct tttgcgtcgg gagatacgtt
     6961 cgacttcggc aaaccggagc cactatttcc agggacgaaa ggcccgcgga cctataccgc
     7021 ggcggtttgc ccggagttgt gtggcaacgc cattcgaatc gatcccgccg tgttggacgg
     7081 tcgtttgtac tacgtgtatt tcgcaggtgg aaacaatgtc acgtccattc ggttgtcaga
     7141 cgcttccgat caactttttc atacgggacc ggatatcaag ggagcgcgag atcccgatga
     7201 agggatcatc aacgaagctc ccgaggcgtt ttcgcgccaa ggccgcctgt acttattctt
     7261 cagcacggcg gattttcggg gaccctatac aacgcgatac atgatcggca acaccgccgc
     7321 ggatttgcgt cgaagtctga cggttgctca tcgactgacg acgcctgctt atggattgaa
     7381 cggcaaactt gtggagaccc atggacacaa ttccgtgacg acctggcgag gcgaaacttt
     7441 caactttttc cacgtgggcg ttttccatca aggcaacctg gttcgacgag atacgtatcg
     7501 tcaacgtatt gtctggaaag acgatggtac cgcggtcaac cagaatgcgg ttcacgtctc
     7561 ttggaactcg cttgggggcg cctatgtcta ttcttttgac cttgttttgc gggatgggtc
     7621 gaccatcggg ccatgcattg gttcggggat tttgggcaag gcaacttccg tgacgtacac
     7681 gggggtatgc aaagacgcga atgatcggtt ggtgcataag gcggaagtgg gggcgttccg
     7741 aatttatgcg tcttcgggag gtggggtata tcacaaggtc ggggaggttt cttacgatgg
     7801 ttatagcgat accgtgtcgt tgaccatcag cccgccctga tcatttttcc aagcacagga
     7861 ttccatcgcg acgcactcga tggcaaagtg tgtggtgtcg tgaaaagatt tggtgcggca
     7921 ctcggtgcag cgaggtgaat cccgaagcca ttgcctcccg aaggcctccc gatgggatct
     7981 tgtcgccgcc cgtgtttgac aaacccaacg atgcgacagg tgcgcgccgc gccttggccg
     8041 aaaatcaggg taagccaacc ttgcatcttc tgtgcattgg caagcggcgt tttgcacggg
     8101 agttgtcgtg ccggccttgg atggggaacg aaaaatataa ataaaatcaa ttagttacat
     8161 ctgtc
//
LOCUS       DAOSOR010000225         8163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8763_length_8163_cov_4.595708, whole genome shotgun sequence.
ACCESSION   DAOSOR010000225 DAOSOR010000000
VERSION     DAOSOR010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8163
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8763_length_8163_cov_4.595708"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PK710_12990"
     CDS             complement(<1..480)
                     /locus_tag="PK710_12990"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOT10685.1"
                     /translation="MGKIFVTGANGFVGSRLVRALVDRGEIVRALVRPGSSIGSLQDL
                     PQDRIEYCPGDITVEHEVYRGLAGCDRMFHVAAVYKMYSPDPRQVLEPAILGTETALE
                     AARKRGTKKVVVTSSVAAIGVNPRPEPMDETWDFNVENTETYIVAKWRSEQKALSFVA
                     "
     gene            807..3305
                     /locus_tag="PK710_12995"
     CDS             807..3305
                     /locus_tag="PK710_12995"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOT10686.1"
                     /translation="MTVESAESEVVSLRKLAKQIADNRRESLTTAHLLVAIASRTSPA
                     ADLLMDRKLDVETLMRLARASTEDSEDAIGRVIRESTTIARNTGFREPGAIHLLVALL
                     RNRKLGAHRALLQSGIDMARLHASSLAIANGFVEPRRVVAREARRASPHVHQHSVPES
                     APAAESTRSRVGKGVIVPLFPPRPTPTAVATPLPRTTEPPAQTLEERPTAHGKSALKP
                     HRPGNRKAKQQDLRLSSTPEERYQLDPKVFPTLAAISRNLTLAALAGQLDPVLCRDME
                     MEQALDVLAKRRANNPLLLGPPGVGKTTIARGLAGHIVDTASSDPRVLLEIPVSHLLS
                     GTAARGSLAERMAAIGKEVSAAQGKIVLVIDEIIQLFQMDNGEVSNDLKLALAQGQLP
                     LIGTCTPEEYRKIVEVDPTLAKGFTVVEIEEPDASRAVEMVLGVCAGLEDHHGLPVEK
                     EAVAAAVGWSVRYLRGSALPEKALSVLDLAGARAKRRGNTSIDDAVVAEVVSEMADIP
                     IERLLQTDRDRMLALDTLLAQNVVGHSEALARIATILRRNAAGLRSQRPIGSFLLLGP
                     TGVGKTETAKAIAHALFHGFDAMTRLDFSEFAEPHALARLIGAPPGYVGHESGGQLTE
                     AVRRRPYQVLLLDEFEKAHREVQQAFLQVFDEGRMTDGRGRTVDFSHTVIVMTSNLGA
                     MEAREAAESRSIGFSSNKGSRSTAVQDVVMTAAKNALPPELYNRIDEILFFQPLTRDE
                     VKQVAHRQLEALSRRLMEAREVQIVWTEEGIDMLLERGGYEPSLGARPVKRAIARLVE
                     APLAEMLLREELKAGDAVQVGATTDGKIELLVLD"
     gene            3598..5883
                     /locus_tag="PK710_13000"
     CDS             3598..5883
                     /locus_tag="PK710_13000"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOT10687.1"
                     /translation="MNHESNHPASIAPDDEQGIDIFAILRTVRKRWMSIAAITLAISL
                     AVTFYSLGQTKIYEAQTMMLFDPNPPRPLGRQVETVVDMGNNNYWNNQEYYETQYRLL
                     KSLRLGKAVVEALGLQHDLAFLQMVPADGKTVPMDPMPSVEDAAQILVNRVTVTPVKD
                     SRLATVAYQDADPARAQRIVMTLAETFVAFNLSDAQQSTSTAVDWLNSQLDKLKEGLD
                     SSELALHEYKLDKKILSTDLDAQSNILREEMQQLSSALTAARIKREELAARHGELAKV
                     NPEDPSDLPATELLQSSVLQRLRTEYINALRERDALLATGKGINHPDVRAAEGNVATN
                     RAAVIAEIRNIQGSVARDLAVVRRQEAGLSGLLQTAKNNAFDLNLLAIEYNRLNRTKE
                     NSEKLYSVVLERSKEGDLARMLQVNNIRVVEPPTLPRAPIRPRMLINTLVGILAGLVL
                     GLGAAFGREMLDRSVKTQADLEGILGLNFLGLIPAVSSDEANKSKYYGYGRRRRHGKT
                     EPAPTSLQPDLIVHQAPLSGPAEAARAIRTNLRFMSADEPFRVVLITSASPSEGKTTV
                     ASNLAIAMAQAGQKTLLLDADLRKPRVHRIFGKSNDRGLTVALLDRTSLTDDLLCTEI
                     PDLFVLPAGPIPPNPAELLQSEKFAELLAELRNRFDRIVIDSSPVGPVTDAAILTTQV
                     DGTVMVVRAFKTTRDVVADAKRTLQTVGGRVVGAVLNAVNLDRSEYKGYVYYSSRGYY
                     RSTEEKSESQPAASNPETKQS"
     gene            6104..7873
                     /locus_tag="PK710_13005"
     CDS             6104..7873
                     /locus_tag="PK710_13005"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOT10688.1"
                     /translation="MNLLLIFSLLAAAVLFPLDAFAETPRSATYDYSPYEQQAIDLTL
                     ERLGLTIEPYPEGKTIERIEIVSLEVIEPRDPAPGFLNIFHTLSRRSTIERELLLHPG
                     SAFRKVVVDETARNLRALPQLSVVLCVPVRGTSPNHVGLVVITKDIWSLRLNSDIQVS
                     SGGLELFVVQPSEINLFGSHHTASARFELNPAAWSTGALYIVPRLTDHLLSASASANI
                     IVNRDSGQTEGSFGGISLGRPLIMSQTPWSWQTSTSWRVQTTRRFVAARLATFDATVT
                     PEDDEIPYEYRTRRWLHRSSVTRSYGWAVKNDVSFGAEYDRRQYALPPSHARAEALQE
                     FRDKRMPTSFTRLSPFVEYAGYTTDFMRVLDFETLGLQEDFRLGHSAIVKLYPASEAL
                     GSTRTLFGAFAAAQYTLPFVDGLVRANIESATEWEPDGLASGSIQGRWRVITPRLGFG
                     RLLVDALVLHRYRNFLNELEILGGQGRLRGYPSNYFVGQDVVVNNLEFRSRPLEILTL
                     QLGGAVFYDAGVIRDRGKPFDVYQSTGFGMRALFPQLDRVVFRVDVGFPLAEGGLPAG
                     VAPAAFYAALEQAFPMPNITSPP"
BASE COUNT         1699 a         2478 c         2231 g         1755 t
ORIGIN      
        1 agcgacgaaa gaaagcgctt tttgctcgga gcgccacttc gccacgatat acgtttccgt
       61 gttttcgaca ttgaagtccc aggtctcgtc catgggctcg ggccgtgggt tgaccccaat
      121 ggcggcgacg ctcgaagtga ccactacttt ttttgttcca cgctttcgcg ccgcctcgag
      181 ggccgtctcc gtaccgagga tcgcgggctc caacacctgc cgcggatccg gtgaatacat
      241 cttgtacacc gccgccacgt ggaacatccg atcacaaccc gcaagcccac ggtaaacctc
      301 gtgctcgacc gtgatatccc cagggcaata ctcgatgcga tcttgcggta ggtcctgcaa
      361 actgccgatc gaactaccgg ggcgaaccaa ggcccgcacg atttcgccgc ggtcgaccag
      421 ggcccgcacc agccgcgatc ccacgaaccc attggcaccc gtgacgaata tttttcccat
      481 gagtccttgg gtatcacggc ccccgcaaga aagggagact tcatgtaaca atgtgaaaat
      541 attgcatttt cattccttcc ttggccgagc ttcgacccac cccatgaggc aataccccgg
      601 gattcatcag cccgatcctc ccccctttca tgcgcgtcga aggcttgccc ggtgcaattt
      661 ctcctcgacg ttccgagcta tcgcttgatt gccctcttaa atttattgta tttcgtgata
      721 aaaacgaaat cgacgccgcc aatcctcaac cgagtaggag acaaacttgg cccggagccc
      781 ccgctattgc gacatatccc ctacccatga ctgtcgaatc cgccgagagt gaggtcgtct
      841 ccctgcgcaa attggccaag caaatcgccg ataaccgtcg agaaagcttg accaccgcgc
      901 atttattggt cgccatcgcg tcaagaacca gtcccgcggc ggatcttctc atggaccgca
      961 agctggatgt ggaaactctg atgcgtctgg cccgagcttc gacggaagac tccgaggacg
     1021 ccatcggtcg agtcatacgg gaatccacga ccatcgctcg gaacaccggt ttccgtgagc
     1081 ctggtgccat ccacctgctc gtggccttgc ttcgcaatcg gaagctcggt gcccaccgcg
     1141 cccttttgca aagcggtatc gatatggccc ggctgcatgc ctcttcgctg gcgattgcca
     1201 acggtttcgt ggaaccccgg agggtagtcg ctcgtgaggc tcgtcgtgct tcgccccacg
     1261 ttcatcagca cagcgtgccg gaatcggcgc ccgcggccga gtcgacgcga tcacgagtgg
     1321 gcaagggcgt catcgtgccg ttgttccctc cgcgtccaac gcccacggcg gtggcgacgc
     1381 ctttgcctag aacgacggag ccgcctgccc agaccctgga ggaacgcccg acagcccacg
     1441 gtaaatcggc tctgaaaccg catcggccag ggaatcgaaa ggccaagcag caggaccttc
     1501 ggctttcttc caccccagaa gagcggtatc agctcgaccc caaggttttt ccgacgctgg
     1561 cggcgatctc acgcaatctc acgctagccg ccctggccgg gcaactcgat ccggtactgt
     1621 gccgagatat ggaaatggag caagcgttgg acgttttggc caaacgtcgc gccaacaacc
     1681 ccttgctcct cggcccccca ggcgtgggaa aaaccacgat tgcacgggga ttggccggcc
     1741 atatcgtcga tacggcttct tcggatcctc gggttttgtt ggaaatccca gtttcccact
     1801 tgctttcagg caccgcggcc cgcggttccc ttgcggaacg catggccgcc atcggcaagg
     1861 aagtcagtgc ggcccagggc aaaatcgtct tggtgatcga tgaaataatt caattatttc
     1921 aaatggataa cggagaggtg agcaacgatc tgaaactcgc tcttgcccag gggcaacttc
     1981 ccctcatcgg tacctgtacg cctgaagaat accgaaaaat cgtggaagtc gaccctaccc
     2041 ttgccaaagg attcacggtc gtggagatcg aagagccaga tgcttcccgt gccgtggaga
     2101 tggtgctggg ggtatgcgcg ggcctcgaag accatcatgg cttgcctgtc gaaaaagagg
     2161 cggtcgcggc ggccgtggga tggtccgttc gataccttcg agggtcggcg cttcccgaaa
     2221 aagcgctttc cgtgctcgat ctagccggcg ctagagccaa acgccgcggc aatacctcca
     2281 tcgacgacgc cgtggtggcg gaagtggttt ccgagatggc cgatattccc atcgaacggc
     2341 tattgcagac cgaccgggac cgaatgcttg cgctcgacac ccttctcgcc caaaacgtgg
     2401 tcgggcatag cgaagccctg gcgcggattg cgaccatctt gcgacgcaat gcagccgggt
     2461 tgcgtagcca gcggcccatc ggttcgtttc tattgctcgg gccgaccggg gtgggcaaaa
     2521 ccgaaaccgc caaggccatc gctcatgcat tattccacgg tttcgatgcc atgacgcggc
     2581 tggatttttc tgaatttgcg gaaccgcatg cgttggcacg actcattggc gcacccccag
     2641 gctatgtggg gcacgaatcc ggtggacaac tcaccgaagc ggtacgccga cgaccctatc
     2701 aagtccttct gctcgatgaa ttcgagaaag cccatcggga ggttcaacag gcgtttttgc
     2761 aggtattcga cgaaggacgc atgaccgatg gtcgcggtcg tacggtcgac ttctcccata
     2821 cggtgatcgt gatgacgtcg aatcttggtg cgatggaggc tcgggaagcg gcagaaagcc
     2881 gttccatagg gttttcgtcg aataaggggt cacggtccac ggccgtgcag gacgtcgtga
     2941 tgacagcggc gaaaaacgcg ctcccaccgg aattgtacaa tcgaatcgac gaaatcctat
     3001 ttttccaacc acttacgaga gatgaggtga aacaggtagc ccatcgtcaa ctcgaagcgt
     3061 tgtctcgacg cctgatggag gcgcgggagg tgcaaatcgt gtggacggaa gaggggatcg
     3121 acatgctttt ggaacggggc ggatacgaac cgagtcttgg ggcgcgtccg gtcaaacgag
     3181 ccatcgcgag actggtggag gctccgctgg cggagatgct gttgcgcgag gaattgaaag
     3241 ccggagatgc ggtgcaggtg ggagcgacca cggatggaaa gatagaattg ttggtgctcg
     3301 actgacatca atcggtgcta caaaacctat ccgtgcgctt cgaaccggac ccggcctccc
     3361 atccccacct cgcccgcgag agcaaatatc ggctccagag ccgatcttcg ttcttgcgac
     3421 cctcccttct cctgactaca aaccccgacc aaagggctga accggaacca ccggggatgc
     3481 aaccaatggt atccaatgat ttcggagtcc acggcatcat cttcagggta gaaccgaagc
     3541 cgatgtgcgg gcaccacatc caatgacaca cgatatcaag acgtttttgg actttccgtg
     3601 aatcacgaat ccaaccatcc ggcttctatt gcccccgatg atgaacaggg gatcgacatc
     3661 tttgcaatct tgcgcaccgt gcgcaaacgc tggatgtcca tcgcggccat caccctggcc
     3721 atatccctcg ctgtcacctt ctattccctc gggcagacca aaatctacga agctcaaacc
     3781 atgatgcttt tcgaccccaa tccccctcgt cccctcggac gtcaggtcga gaccgtggtc
     3841 gatatgggca ataataacta ttggaataat caagaatatt atgaaactca gtatcgtctg
     3901 ctgaaatccc tgcgcttggg aaaagcagtg gtggaagccc ttggattgca acacgatttg
     3961 gcatttttgc aaatggtgcc ggccgacggc aaaaccgttc cgatggatcc gatgccttcg
     4021 gtcgaggacg ccgctcagat tcttgtcaat cgcgtgacgg ttacccctgt caaagacagc
     4081 cgtcttgcca cggttgccta tcaggacgcc gatccggctc gtgcgcaacg tatcgtgatg
     4141 accttggccg agacgtttgt ggctttcaac ttgtcggatg cccaacaatc cacgagtacg
     4201 gccgtggatt ggctcaacag ccagctcgac aagctcaaag aggggctcga ctcgagcgag
     4261 cttgcgctgc acgagtataa gctagacaaa aagatcctct ctaccgacct ggatgcccaa
     4321 tccaatatcc ttcgtgagga aatgcagcag ctcagctcgg ctttgaccgc ggcgcgaatc
     4381 aagcgagagg aattggccgc gcgccatggc gagttggcca aagtcaatcc ggaagaccct
     4441 tccgacctgc ccgccacgga acttctccaa agttccgtgt tgcaaaggct tcgaaccgaa
     4501 tacatcaacg cccttcgaga acgggatgcg ctgctagcga caggcaaagg cataaaccat
     4561 ccggatgttc gcgccgccga aggaaacgtc gccaccaacc gcgctgccgt catcgcggaa
     4621 atccgcaata tccaaggctc cgtagcgcgc gatcttgccg tcgtgcgacg acaagaagcc
     4681 gggctgtcgg gattgctcca aacggcaaaa aataacgcct tcgacctgaa tcttctcgcc
     4741 atcgagtaca atcgcctcaa ccgtacgaaa gaaaatagcg aaaaactcta ttccgtcgtg
     4801 ctggagcgtt cgaaagaggg cgatcttgcc cgcatgcttc aggtcaacaa catccgtgtc
     4861 gtagaacctc ctaccttgcc tcgggctccc atccgtccac gaatgctcat caatacgctc
     4921 gtgggcatcc tcgcgggatt ggtccttggt cttggcgccg cgtttggaag ggaaatgctc
     4981 gaccggtccg tgaaaaccca ggccgatctc gaaggaattc tcggcctcaa cttcctgggt
     5041 ctcattcccg ccgtctcctc cgatgaggcc aataaatcta agtattatgg gtacgggcga
     5101 cgacgaaggc atgggaaaac cgagccggct cccacttcct tgcagccgga cttgatcgtt
     5161 caccaggccc ctctcagtgg acctgcggaa gccgcgcgcg ccattcgaac caacctgcgt
     5221 ttcatgtccg ccgatgagcc ttttcgcgtg gtgctcatca ccagcgccag cccatcggaa
     5281 ggtaaaacca cggtggccag caacctcgcc atcgccatgg ctcaggcagg gcaaaaaacc
     5341 ctgttgctcg acgcggactt acgaaaaccc cgggtccacc ggattttcgg aaaatcaaac
     5401 gatcgggggc tcacggtggc tttgctcgat cgaaccagcc tcaccgacga cttgctctgc
     5461 acggagattc cggatctatt cgtgctcccg gcggggccaa ttccccctaa cccagcggaa
     5521 ttattacaga gcgagaagtt cgccgaactt cttgccgagc ttcgaaaccg tttcgaccgc
     5581 atcgtgatcg atagctctcc cgtgggtccc gtgaccgacg ccgccatcct caccacccag
     5641 gtcgatggca ccgtcatggt cgttcgcgct ttcaaaacca cccgcgacgt ggtggccgat
     5701 gcaaaacgaa ccctccaaac cgtcggagga cgcgtcgtgg gtgcggtgct caacgcggta
     5761 aatctggatc ggtcggaata taagggatat gtgtattatt cgagccgggg gtactataga
     5821 agcaccgagg agaaatcaga gagccaaccc gctgcctcga atcccgaaac caagcagtcc
     5881 tagccatggg caagccgtgt ggagccacgg cgcgcgcctt ccattcccat cgtgctccgt
     5941 acttatgtaa cctccctaga tattcccctc ttcgaccaca ccaatttccg ccttccctcc
     6001 aaacaccaag gttcgtcaag gccctgaccc ccttgtgctt ccgccggact ttctcgaaat
     6061 cccgtcccct tgcccgcaac ctcgtctatc atgactccac ggaatgaacc ttctgcttat
     6121 cttttccctc cttgccgcgg ctgttctctt tccgctcgac gcttttgctg aaacgcctcg
     6181 ctccgccacg tacgattact ctccttatga acaacaggcc atcgatctca ccctcgagcg
     6241 cctcggtctc accatcgaac cgtatcccga aggaaaaacc atcgagcgaa tcgaaatcgt
     6301 atccctcgaa gtcatcgagc cacgggatcc agctccagga tttttgaata ttttccatac
     6361 gttatcgcgt cgctccacca tcgagcggga gcttctcctg cacccgggct ctgccttccg
     6421 gaaggtcgtc gtcgacgaaa cggctcgtaa tctccgcgcc ctgccccaat tgtccgtcgt
     6481 gctatgcgtg cctgttcggg gcacctcccc caaccacgtg gggcttgtgg tcattaccaa
     6541 agatatttgg agccttcgtc tcaactccga tattcaggtt tccagcggtg ggcttgagtt
     6601 atttgtggtg caaccctcgg aaatcaacct attcggctcg catcataccg cttccgctcg
     6661 attcgaactc aaccccgccg cctggtccac gggcgctttg tatatcgttc ctcgcctgac
     6721 cgatcacctg ctttccgcga gtgcaagcgc caatatcatc gtcaatcggg atagtgggca
     6781 aaccgaaggc tcttttggag gaatctcgct gggccgtccg ctcatcatga gccaaacccc
     6841 atggtcatgg cagaccagca cctcctggcg tgtgcaaact acccggcgat tcgtggccgc
     6901 ccgtctggcc accttcgatg ccacggtgac cccggaggac gacgagatac cctacgaata
     6961 tcggacaagg cgatggctgc accgttcgag tgtgacacgg tcctacggat gggccgtgaa
     7021 gaatgatgtg tctttcggtg cggaatatga tcgtcgccag tacgcgctgc ccccttccca
     7081 cgcgagagca gaggcgttgc aggagtttcg agataagcgg atgccgacca gtttcacgcg
     7141 tctgagcccg tttgtggaat atgccgggta tacgacggac ttcatgcggg tgctggactt
     7201 tgaaaccctt ggattacaag aagattttcg gttggggcat tccgctatcg tcaagctata
     7261 tcctgcttcg gaagcgcttg ggtccacccg tacccttttt ggtgcgttcg ctgcggcgca
     7321 gtatacgctg ccgtttgtcg atgggctcgt gcgggcgaat attgagagtg ccacggaatg
     7381 ggagcccgac ggtcttgcga gcggttcgat tcaaggccgt tggcgagtga tcacgccgcg
     7441 tctaggcttt gggcgtctgt tggtcgatgc gttggttctt catcgatatc gaaatttcct
     7501 caatgaattg gagatcttgg gtgggcaagg gcgattgcgg gggtatccga gcaattattt
     7561 cgtggggcag gacgtggtcg tcaacaacct tgaattccgg agccgccccc tcgagatcct
     7621 caccttacaa cttgggggcg cggtgtttta tgatgctggg gtgatacggg atcgtggcaa
     7681 acccttcgac gtctaccaat ccacgggttt tggcatgcgt gcgctttttc cccaactcga
     7741 tcgggtcgtt ttccgggtcg atgttggctt tcctttggca gaagggggac tgcccgcagg
     7801 ggtggccccg gctgcgttct atgccgcttt ggagcaggcc tttccgatgc ccaacatcac
     7861 ctcgccccct tgatgggcct ctgcatagcc gtcggattca accatcttcc gccctgacag
     7921 atcccctcat tttttcctga atgggaaagg gatacgagct gaggattctt gggtcttgat
     7981 ttgggctcgg tccttgactc cccccttgga gcacccccaa cagttgccct gggctcataa
     8041 gcattggact ggtgttctgc ggtgaacgat atgggatgac cgtgaccggt tttgtgtggc
     8101 gtgcgccgtg accgtgaccg gttttgtgtg gcgtgcgccg tgaccgtgac cggttttgtg
     8161 tgg
//
LOCUS       DAOSOR010000226         8163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8764_length_8163_cov_3.710039, whole genome shotgun sequence.
ACCESSION   DAOSOR010000226 DAOSOR010000000
VERSION     DAOSOR010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8163
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8764_length_8163_cov_3.710039"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PK710_13010"
     CDS             <1..429
                     /locus_tag="PK710_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10689.1"
                     /translation="LADNDWPALWLIAMAMESECDVLPWHADSTAFRICKQISVLQRQ
                     QEEAALRKRLDEIIADGLSLSEMESAKRKIVEMEARRMSDPKAIANMLSRWDASQADG
                     VGDRLRAVTDIRSAARRFLDAKSVKGVLVPMEPQKSNIVN"
     gene            542..1198
                     /locus_tag="PK710_13015"
     CDS             542..1198
                     /locus_tag="PK710_13015"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT10690.1"
                     /translation="MKPEVRYLSGFALIAVGAAGLVGMNVWTVPELPKGRDGCQEPTG
                     SEQRAVVSATVEQAVSAEAPVPRLQEMADAGAVIGDAEADVMRQEITSWTTVPDGTEL
                     PSIRFEPQSQVLSAEMSRNVEPIAAYLRNHFGMKVVLVGHGDEGMDAAEYVQLGRRRS
                     SAVLRMLVDLGVTVARIGIEPPNVEEGRIVTKGIPPGAMELRIEPRFVQPKKGDNDGP
                     "
     gene            1188..1940
                     /locus_tag="PK710_13020"
     CDS             1188..1940
                     /locus_tag="PK710_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10691.1"
                     /translation="MAPEWVILVTSTVCALLMTLGGVLVGWAKGEMGCWSHTQKLQAS
                     LSSAQRSAQEALAKADAAKVEADSLRTKANQHAEDISRDTKAPASEELMARLEATEAK
                     LSKALISADEADKEFTIVREKLDKTEKRLAEANRRLADRGDDDGGMAGALEAERTTTK
                     RLTERLEKVEKERDDAMERVKSLASKLRAQEDAATKAPSEQQMEALRQEVEVAKERVA
                     VSERVMEGVRARSAMLSQELKKVKAELAALKG"
     gene            complement(1962..4196)
                     /locus_tag="PK710_13025"
     CDS             complement(1962..4196)
                     /locus_tag="PK710_13025"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT10692.1"
                     /translation="MSREPKAKKPPMGPTDAEGTSSSGADAWGTYFLGPYRVVDEIGV
                     GGMATVELARMDGAGGFRKWVAIKRIHPHLTEEQQFINMFLDEARIAASISHPNVAQV
                     FDLGKDGNTYWIAMEYLHGEPLREIMRINEEQETSIQPELAARLIADAAEGLHAAHEL
                     RGKNGQPLNLVHRDVTPHNLFITYDGVVKVVDFGIAKVTGSLSSTHAGTIKGKVAYMS
                     PEQVRGRPIDRRADVFALGVVLWEMTTGRRLFRVDSDLATLSRVEACVVPPPSALDAD
                     YPPELEAVVLKALQREPETRYQTARELSRALQHFLMGARTFVGPEDISAYVSDMFSDR
                     IRKREDHLRWAAEVTQTISIRQALTEEAPGDMLDLSKLPRDTGESDVLDFDTLRDQPA
                     PEVHGIPSPLGSGDNPPELEKTQAQRAGLASPSPASALFTPPPRHSDSLSSTEAVIIS
                     DPSVENAHDDDDEEAATLVANSPLAMQAIATELLRSPIGSPLGDPTASTDGMETRVQP
                     AQFTAPAPILDPSRRPQPSATDWASPRPIPRTFDVHSSPAAPTMQLSAVRPGISPLVV
                     FAAAVGAGFIAAGLGAIWIVKNKTETPNDPPSVTLSSPLASPPLSTQLPVTTKTVRTV
                     ASPPTPSEQPTPSVPSARSTQDQSTYPSGDRKKTSASKTPDPPIDPGSLDETARPGFL
                     SIHCKPACDQIVAGGRTFSGSISRVPMQPGSYRVHCIRGSVTKILSVIVVSDEHSSQG
                     VQME"
     gene            complement(4320..6635)
                     /locus_tag="PK710_13030"
     CDS             complement(4320..6635)
                     /locus_tag="PK710_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HOT10693.1"
                     /translation="MSNRWLRFGLLVGCLTACGTAGDNTRNYASAKPDAADDVIIDAT
                     GDPSLDKDTPPPKETGPEVSADTEPDTPADVVETGPETTTPDVEQEAEAEAGCPTGTT
                     GPNCDACADGYFECGASCIENCEDCPGKTLNCDGQCVTACHTCPGQPAACGQTCMSSC
                     ATCDNKPLDCQGTCIANCDACPEKPLQCNAKCVATCNACPGQPATCGQACLASCATCD
                     NKPLDCQGTCVSECAQCAGADYSCQGACIASCMSCAGQQATCETTHECLSTCSSCSGS
                     CLECPAGYHACPNGCHIDGQDDPALGCAYTCDDQPCPIPENGDSICSMGNCDIVCDTP
                     AGFIKVGSQCLCDADHGFVKHPTLPLCVCENGFKGCGTFCASVTDPAYGCSHTYCLAC
                     PTPPHTLTTFCNASGECDFTCDDASGYIKNSSGTDCVCPSGLFDNGTSCGCEPGLKYC
                     NGSCVSASTPNNGCAGPSCDPCPTPAHATAICDAGACSFVCDASGHWKKSGNQCICDN
                     GYTEVGGECVTACLGSTYGSSCYWYVGTAATWDQAQANCAAQGGYLASIANSAENTFV
                     RSLGSDRIWIGLRDHSTDQRTFSKADDCTQNIMLSGDGGTYFGSTTNSWDDIDPCNIS
                     SDSDDIFGIEVTTPGIYVFSTANTSWDTILGLYRRVANTSGYTSCLGSNLACDDDSGP
                     GTRSLIIRYLDTGSYTLVLDGYYYSWGSYQLDVRRFDLVDGTIHQWANWASDEPNNSS
                     SAEKCVEMNTAGTWNDLSCASTRSYVCERPM"
     gene            6739..8130
                     /gene="glmM"
                     /locus_tag="PK710_13035"
     CDS             6739..8130
                     /gene="glmM"
                     /locus_tag="PK710_13035"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOT10694.1"
                     /translation="MTSENASTDRELFGTDGIRGVANVAPMTPELALRLGRAITYVAG
                     KGKSRRPRIVIGKDTRLSGYMLETAMASGICAMGGRVMLSGPIPTPAVAHLTVSMRAD
                     AGVVISASHNPYSDNGIKVFGSDGFKLPDEQEAEIERLLRSSDIDASAPTGEGIGSAI
                     RVDDAKGRYIVFAKQSIPKEYTLDGLRIVVDAAHGAAYSVAPSVFMELGADVISLGCE
                     PDGKNINDKVGALYPASVQQQVVNHRAHIGIALDGDADRVIIVDDAGHIVDGDAIMAL
                     YGIERLRAGTLPKATVVATVMSNLGLERAIENAGGKLVRTAVGDRYVVEEMRKSGYMF
                     GGEQSGHLIFLEHASTGDGIIAALQVLLIMLRRGRALSELVPGCLQRVPQLLKNVSLS
                     DRKPIQEMPLLMRAIQKVENELGREGRVLVRWSGTEPKLRVMVEGPDEARIATYVNDM
                     IEQASRDIPPSNN"
BASE COUNT         1763 a         2100 c         2565 g         1735 t
ORIGIN      
        1 ctggctgaca acgactggcc ggcgttatgg ttgatcgcaa tggcgatgga atcggagtgt
       61 gacgttcttc cgtggcatgc cgattcgacg gcgtttcgaa tatgcaaaca gatatccgtt
      121 ttgcagcgac agcaggagga agcagcgttg cgcaagcgtc tggacgaaat catcgccgac
      181 ggcctgagcc tatcggaaat ggaaagcgcg aagcgcaaga tcgtcgagat ggaagcgagg
      241 cgcatgtccg atccgaaggc gatagcgaac atgctttcgc gatgggatgc atcacaagcc
      301 gatggggtag gcgacagact gcgtgcggtc acggatatca ggtcggcggc gagacgattt
      361 ttggatgcaa aaagcgtcaa gggggtgctt gtccccatgg aaccccaaaa gtctaacatc
      421 gtgaattaat tgaataatta taggttccgg cgaactgtgg ggtgggattg ggcttggcgg
      481 aacgaaaagc gcccaggatt gtttgccaag caggcatcga tcgggtgtat gaacggcatg
      541 gatgaaaccg gaggtgcgtt atttaagtgg ttttgcgctg atcgcggtgg gcgcggcagg
      601 gcttgtgggc atgaacgtgt ggacggtgcc ggaactgccg aagggccgtg atgggtgtca
      661 ggagcccacg ggcagtgagc agcgcgcggt agtctcggcg acagtcgaac aggctgtatc
      721 ggcagaggct cccgtgccga ggctgcagga aatggcagac gctggtgccg tgataggcga
      781 tgccgaagcc gatgtgatga ggcaagaaat cacgtcgtgg acgaccgtgc cggatggcac
      841 cgaattgcca tcgattcgtt tcgagcctca aagccaggtt ctcagtgcgg agatgagccg
      901 gaacgtggag ccgatcgcgg cctatttgcg caaccatttt gggatgaaag tggtgctcgt
      961 ggggcatggc gatgagggca tggatgcagc ggagtatgtt cagcttggcc ggcgtaggtc
     1021 ttctgctgtg ctgcgcatgc tcgtggattt gggggttacc gtggcacgga ttggtattga
     1081 accgcctaac gtggaagagg ggcggatcgt aacgaaaggc ataccaccag gggcgatgga
     1141 gctgcgcatt gaacctcggt tcgtgcaacc gaagaaaggg gacaacgatg gcccctgagt
     1201 gggtgatcct cgtaaccagc acggtatgtg ctctactcat gacccttgga ggggtgctcg
     1261 ttgggtgggc gaagggggag atggggtgtt ggagccatac ccaaaaactg caggcatctc
     1321 tatcttcggc gcagaggagc gcacaagaag ccctggcgaa ggcggatgca gccaaggtcg
     1381 aggcagattc tcttcgcacg aaggccaatc aacacgcgga ggacatatcg cgcgatacga
     1441 aggctcctgc ttcggaagaa ctgatggctc gtctcgaagc gacggaagcc aaactgagca
     1501 aggcattgat ttccgccgac gaagccgaca aagagtttac gatcgtacgc gagaaactcg
     1561 acaagacaga gaaaagactc gccgaggcca atcgtcgttt ggcggatcgc ggagatgacg
     1621 atggggggat ggctggcgcg ctcgaagccg agcgaaccac gacgaaacgg cttacggaac
     1681 gactcgaaaa ggtggaaaaa gagcgtgatg acgccatgga gagggtcaag tcattggctt
     1741 ccaagttgag agcacaagag gatgcagcga ccaaggcgcc ctcggaacag cagatggagg
     1801 cattgcgtca agaagtggaa gtcgccaaag agcgagttgc ggtttcggaa agagtgatgg
     1861 agggagttcg ggcgcggtcg gcgatgcttt ctcaagaact caaaaaggtg aaagcggaac
     1921 ttgcggcgct caaaggttga gaacgtcgtg tgcggcgggg gtcattccat ctgcacgcct
     1981 tgagagctgt gttcatcgga aacgacgatc acggacaata tcttggtgac gctgccacgg
     2041 atacaatgaa cgcggtagga accgggctgc atgggaactc gggagatcga gccggagaag
     2101 gttcttccac cggccacgat ctggtcgcat gcgggtttgc aatgaatgga aaggaacccc
     2161 gggcgtgcgg tttcgtcgag actgcctgga tcgatgggag gatcaggggt tttggaagca
     2221 gaggtttttt ttcgatcccc gctgggatac gtagattgat cttgtgtaga gcgtgccgac
     2281 ggtacggaag gggtgggttg ttcggacgga gttgggggcg atgcgacggt tcgaacggtt
     2341 ttcgtggtaa cgggcaattg cgtggacagt ggtggggagg caagtggtga cgatagcgtt
     2401 accgagggcg ggtcgtttgg tgtttcggtt ttgtttttta cgatccagat ggcgccgagc
     2461 cctgcagcga tgaaaccagc tcccacggcg gcggcgaata cgaccagcgg tgaaatgcct
     2521 ggtcgtactg cggaaagttg catggtagga gccgcgggcg aagaatggac atcgaaggtg
     2581 cgcgggatgg gtctgggaga tgcccaatcc gtggcgcttg gttgaggacg acgggatggg
     2641 tcgaggattg gcgcgggagc ggtgaattgt gcgggttgaa cgcgcgtttc catcccatcg
     2701 gtcgaagcgg taggatcgcc caaaggactg ccgatggggc ttcgcaatag ctccgtggcg
     2761 atcgcttgca tggcaagcgg ggagttcgca acgagcgtgg cagcctcttc gtcgtcgtca
     2821 tcatgcgcat tctccacgga tgggtcggaa atgatgactg cttccgtgga tgaaaggctg
     2881 tcgctgtggc gtggtggcgg cgtaaacaaa gcggaggcag gcgatggtga ggcaaggcct
     2941 gcgcgctgtg cttgggtttt ttcgagttcg ggagggttgt cgccggaacc caggggggaa
     3001 ggtattccgt ggacttcggg agctggttga tcgcgcaacg tgtcgaagtc caaaacgtcg
     3061 gattcgcccg tgtcccgcgg aagcttggac agatcgagca tatcgccggg agcttcctcc
     3121 gtgagagctt gccgaatcga gatggtttgt gtgacttccg cagcccaacg caaatggtcc
     3181 tctcgctttc ggatgcgatc ggaaaacata tccgagacgt aagcggagat gtcctcggga
     3241 ccgacgaagg tgcgggcgcc catcaggaag tgttgaaggg ctcgcgatag ttctcgggcg
     3301 gtttggtagc gcgtttcagg ctcccgttgc aatgccttga gcacgacggc ttcgagttcc
     3361 gggggataat ctgcatccaa agcggaagga ggtggcacca cacaagcttc gacacgactc
     3421 aaggtggcca aatccgaatc cactcggaag agcctccgcc ccgttgtcat ttcccacagc
     3481 accacaccaa gcgcgaacac atcggctcga cgatcgatcg gacggcctcg cacttgttcc
     3541 ggagacatat aggccacttt gcctttgatg gtgccggcat gggtgctcga aagactgccg
     3601 gtgactttgg cgatgccaaa atccactacc ttgacgacgc catcgtaggt gatgaaaagg
     3661 ttgtgagggg tgacatcgcg atgaaccagg ttcaacggtt gaccattttt cccgcgcaac
     3721 tcatgcgccg cgtgaagccc ttcggccgcg tctgcgatga gtctcgccgc aagctcgggt
     3781 tggatcgacg tctcttgttc ttcgttgatc cgcatgattt cccgcaacgg ctcgccgtgc
     3841 aggtactcca tcgcgatcca ataggtgttt ccgtctttgc caaggtcaaa gacctgcgcg
     3901 acattggggt gcgagatgct tgcggcgatt ctcgcctcat cgagaaacat gttgatgaac
     3961 tgctgttcct cggtcagatg cggatgaatt cgtttgatcg ccacccattt tcgaaagccg
     4021 cccgcgccgt ccattcgcgc aagctccacc gtggccatcc ctcccacacc gatttcatcc
     4081 accacgcgat aggggccaag aaaataggtc ccccaagcgt ccgcgccgga actggaagtt
     4141 ccttctgcat ccgttggccc catgggcggc tttttggctt ttggctctcg actcatgctg
     4201 gcaacggcac cacgtacacg tagaagataa cattagcctc aagccgcggt gggaggctag
     4261 tcgctagcga aaggaaacgc tccttggaat gaccgatttg tccaatcatg aattaatttt
     4321 tacatgggac gctcacatac ataagaccgc gtagaagcac agctaaggtc attccaggtg
     4381 cctgcggtat tcatttccac gcacttttcc gctgaactag agttgttggg ctcgtcggaa
     4441 gcccaatttg cccattggtg aatcgtaccg tccacgaggt cgaaacggcg tacatccagt
     4501 tggtagctac cccaggaata atagtagccg tccaacacca gcgtgtacga gcccgtgtcc
     4561 aagtaacgga tgatcagcga tcgggtacca ggtcccgagt cgtcgtcaca ggccaggttg
     4621 gatcctaggc aagatgtgta tcctgacgta ttggcgacgc ggcgatacag tccgagaatc
     4681 gtgtcccaac tcgtgttggc ggtggaaaat acgtagattc ccggagtcgt tacctcaata
     4741 ccgaagatat cgtcactgtc ggaggaaata ttgcagggat cgatgtcgtc ccaactattc
     4801 gtcgtggagc cgaaataggt tcctccatca ccgctaagca tgatattttg cgtgcaatca
     4861 tcggctttgg agaaggttcg ttgatccgtc gaatgatcgc gtaatccaat ccagattctg
     4921 tcgcttccca ggctccgtac gaaggtgttt tccgccgagt tggcgatcga ggcaagataa
     4981 ccgccttgtg ccgcgcagtt cgcctgcgct tgatcccagg tggctgccgt gcccacatac
     5041 caatagcaac tcgaaccgta cgtgcttccg agacaagccg ttacgcactc tcctcccact
     5101 tccgtgtagc cgttgtcaca gatgcattga tttccagatt ttttccaatg tccggaagca
     5161 tcgcaaacga aactgcaggc ccccgcatcg catatcgccg tggcatgggc cggtgtgggg
     5221 catggatcgc aggatggtcc cgcgcaaccg ttgtttggtg tcgaagcgct gacgcaactg
     5281 ccattgcagt atttcaatcc gggttcacaa ccgcatgacg tgccattgtc gaataagccg
     5341 cttgggcata cgcagtcggt gccggacgag ttcttgatgt acccagaagc gtcgtcacac
     5401 gtaaaatcgc attcgcccga ggcgttgcag aatgtcgtca gcgtatgagg gggagttgga
     5461 cacgccaaac aataggtatg ggaacagccg tacgcggggt ctgtcacgct ggcgcaaaac
     5521 gtaccgcatc ctttgaaacc gttttcgcat acgcaaagcg gaagcgtggg gtgtttgacg
     5581 aaaccatggt ccgcatcaca taaacactgg cttccgacct tgatgaatcc agcgggcgtg
     5641 tcacaaacga tatcgcagtt gcccatcgaa caaatcgaat cgccgttctc agggatcgga
     5701 caaggttgat cgtcgcatgt ataagcgcat cccagtgccg ggtcatcctg cccatcgata
     5761 tgacaaccgt tgggacaagc gtggtatccg gctgggcatt cgagacagct accggagcag
     5821 gaactgcaag tgctcaggca ctcgtgggtc gtttcacagg tagcttgttg ccccgcgcaa
     5881 ctcatgcatg aggcgataca agcgccttgg caagaatagt cggcgccagc gcattgcgca
     5941 cattccgaca cgcatgttcc ttggcaatcg aggggcttgt tgtcgcaagt cgcgcacgaa
     6001 gccaagcacg cttgcccgca ggtggctggt tgccctgggc atgcattgca tgtcgccacg
     6061 cattttgcgt tgcattgcaa gggtttttcg ggacaagcat cgcaattggc gatgcacgtt
     6121 ccctggcaat cgaggggctt gttgtcgcaa gtcgcgcacg aagacatgca cgtttgccca
     6181 caggcggctg gttgtcccgg gcaagtatgg caagccgtca cgcactgtcc gtcacagtta
     6241 agtgtttttc ccgggcaatc ttcgcagttt tcaatgcagg aggctccgca ttcgaagtat
     6301 ccgtcggcac acgcatcaca attcggtccc gtggttcccg tagggcatcc tgcctcggct
     6361 tccgcttctt gttcgacgtc gggcgtggta gtttccggcc ccgtttccac cacatcggca
     6421 ggcgtatcgg gctcggtatc cgctgaaacc tccgggcccg tctctttcgg ggggggagtg
     6481 tctttgtcga gcgatgggtc gccggtggca tcgatgatga catcatcggc cgcgtcgggt
     6541 tttgcggatg cgtagtttct cgtgttatcc cccgctgtac cgcaggcagt caggcatccc
     6601 acgagcagtc caaaccgaag ccaacggttg ctcatggaag ggatcatacc atgagtcatg
     6661 cctcgatcga acgtgggagc cgttcttaat tcagttcccc cttggcgtcc aagggcgaga
     6721 ttgtgtaacg tcccggagat gacgtccgaa aacgcatcca ccgaccggga attatttgga
     6781 acagatggga ttcgtggcgt ggcgaatgtc gcgccgatga ctccggagct ggctctgcgc
     6841 ctgggccgtg ccattacgta cgtggcaggc aaaggcaagt ctcgccgacc gcggatcgtc
     6901 ataggaaaag atacgcgatt gtcggggtat atgcttgaaa cggccatggc gtccggtatt
     6961 tgcgccatgg ggggcagagt catgctctcg gggcccatcc caacacccgc ggtagcccac
     7021 ctcaccgtca gtatgcgcgc cgatgcggga gtcgtgattt ccgccagcca caatccgtat
     7081 tcggataacg gtatcaaagt ctttggcagc gatggcttca agctgccgga cgaacaagaa
     7141 gccgaaatcg aaagattgct gcgttcgagc gacatcgatg ccagcgctcc taccggagaa
     7201 ggaatcggtt cggcgattcg agtcgatgac gcaaaagggc gttacatcgt tttcgcgaaa
     7261 caaagcattc ccaaagaata caccctcgat ggcctgcgta tcgtcgtcga tgcagcccat
     7321 ggcgcggcct acagcgtggc accctcggtt ttcatggagc ttggcgccga tgtcatttcc
     7381 cttggttgtg agcccgacgg aaaaaacatc aatgataagg tgggggcttt gtatccggcc
     7441 tccgtgcaac aacaagtcgt caatcatcga gcgcatatcg gtatcgcgct tgatggtgat
     7501 gccgatcggg tcatcattgt cgatgatgcg gggcatatcg tggatggcga tgccatcatg
     7561 gcgctatacg gtatcgagcg gctgcgagcg gggacgttgc cgaaggctac ggtcgtggcc
     7621 acggtgatgt cgaatctcgg acttgaacga gccatcgaaa acgctggagg aaagcttgtt
     7681 cgaacggccg tgggggatcg gtacgttgtg gaggagatgc gcaaatccgg ctatatgttt
     7741 ggaggggagc aatcgggtca cctcattttc ctcgaacatg cctccacggg cgatgggatc
     7801 attgcggctt tgcaggttct gttgatcatg ctgcggcgcg gtcgcgctct ttcggaactc
     7861 gtgccgggat gcctgcagcg tgttccccaa ctgctcaaaa acgtttcgct ttccgatcga
     7921 aagcccattc aggaaatgcc gttgttgatg cgtgctatcc aaaaggtcga aaacgaactt
     7981 gggcgtgaag gtcgcgtgct cgttcgatgg agcggaacgg aacccaaatt gcgcgtcatg
     8041 gtcgaaggtc ccgatgaagc tcgcattgca acctacgtca acgatatgat cgaacaagcc
     8101 tctcgcgaca tccctcccag caacaactga cgtggacatg cttcatgcgc aggggcttcg
     8161 acg
//
LOCUS       DAOSOR010000227         8162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8767_length_8162_cov_4.641668, whole genome shotgun sequence.
ACCESSION   DAOSOR010000227 DAOSOR010000000
VERSION     DAOSOR010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8162
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8767_length_8162_cov_4.641668"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PK710_13040"
     CDS             <1..680
                     /locus_tag="PK710_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10695.1"
                     /translation="GGSGGGEPGGSGGGEPGGSGGAPTGGSGGAPTGGSGGGPSSGYG
                     NLTKVSFSSGFIFDESKLNDQSYLQAHMSGIQMTPAFTGSYTSANAAIPPGGAQTLAL
                     AIHFAPNGLGVLQQSGQGQTVVAPMIQFMFASDVVASGPVDVGLEQTSGAMLLVMDPV
                     GANDACVAAIGIGQVQVTQAQNTNAADGGQLAFNGSNIALYHPSDTPYGNIETELAQQ
                     IAVCPFK"
     gene            742..2361
                     /gene="nadB"
                     /locus_tag="PK710_13045"
     CDS             742..2361
                     /gene="nadB"
                     /locus_tag="PK710_13045"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOT10696.1"
                     /translation="MTLSCDYLVIGSGVAGLAFALEAANHGHVIVVTKQRADESNTRY
                     AQGGIASVLSQKDSFASHMQDTLTAGAGLCHPLAVQVCVEEGPARIDWLVQQGVEFST
                     KPNGTQKTDLDLGREGGHSERRVAHAGDMTGKEIVRVLDAAARAHPNIQLLEHHTAVD
                     LIMLPEYGGPDICGGAYVLDASSEHVLTIRAHATMLCTGGSGKVYLYTSNPDVATGDG
                     VAMAYRAGAEIANMEFYQFHPTCLFHSQAKSFLISEALRGEGAILRLLDGTPFMKKHH
                     ERADLAPRDIVARSIDYEMKRTGSDYVLLDITHRPASFVRERFPGIHATCQQWGIDIT
                     STPIPVVPAAHYMCGGITTDMHGRTTIPGLWALGEVACTGLHGANRLASNSLLEGLVL
                     AHRASLKLSSQIDALRSRPHVTVKDWEVGDAVSSDEKVIVSQNWDELRRFMWNYVGIV
                     RSATRLQRAARRIALLQDEIHEYYWRYLVTRDLIELRNIATVAELIVASAASRQESRG
                     LHFTLDYPDKDDRFLDDTVMKRGVSARLRGV"
     gene            complement(2386..2598)
                     /locus_tag="PK710_13050"
     CDS             complement(2386..2598)
                     /locus_tag="PK710_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10697.1"
                     /translation="MGSVGPDVVDAAGEDACGEVVVSGERAFREVGELAPERAGAEQQ
                     ASSIDTIHKHHNAMASAYPQRGASAK"
     gene            2657..3391
                     /locus_tag="PK710_13055"
     CDS             2657..3391
                     /locus_tag="PK710_13055"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOT10698.1"
                     /translation="MAIAASPYEDTRILVLMYHAFDRGKEPLTVDGWQFERQLLWLRD
                     NGIAIVPTSALAMFLRKEKPLPKRAAVITIDDGDKSVHAVAWPILKKLEVPFTLALPT
                     KLMNENKKHRMLTWDQVREMVDSGLCEVASHGHEHKSIVHFSNEQAVRQLETSRDILE
                     KETGQRPIAFVYPLGAFDARTSALVEKVGYSMAFKASGAPIGMDCCDPYALPRMGIVH
                     QDTVWSLGWYFSPSFLGRMTRLPRGR"
     gene            complement(3432..5156)
                     /locus_tag="PK710_13060"
     CDS             complement(3432..5156)
                     /locus_tag="PK710_13060"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HOT10699.1"
                     /translation="MSLRNKSAARTIAFRLSLPLIGIIFGACVLDAEGRGTSSVSCGN
                     EILEEGEECDGSLVADMTCMTLAYSGGELRCSKTCTFDVSRCGRCGDGIREGNEQCDG
                     NDLAGQTCASVIGLADGELRCTSQCRWDLTNCSKCLNGVREGNEQCDVIVPSTATCET
                     VVGRQSGEVRCSDDCTFDTTRCHTCGDGIIEGPEECDGNELGAATCETFHYASGVLSC
                     KNDCTLNKSQCLVPSADWGDLQWRFRRLITVPNKLVDETLSGFPLLVTVVDPSLAAHV
                     TSSADFRFTLGGSTTVLAHEIVSWNSENGALVAWVRLPALSALFDTKILMYYGNPSPP
                     SPAPSIPVWTDGFSAVWHLDEEDAATSHVNSAADRFHGAKAGNHAVAGKVGRAQRFDG
                     QNQHIQIVKADGISLGNSNVTISAWIRTKAHQRAGLVTKSPAAQWPPESKAFGMLDNV
                     VVVTNGFGVWLSSTKPVNDNQWRYVVWTQQKDVVGLNERWILYVDGVQNGKGDYTTGE
                     DGTGHSLWIGSPMGTLLTNYWKGDIDEVSISNVARSAAWIAASYRNQNAPESFIIVGD
                     EQTVLVGP"
     gene            5246..5926
                     /locus_tag="PK710_13065"
     CDS             5246..5926
                     /locus_tag="PK710_13065"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOT10700.1"
                     /translation="MGVFFQSATSNAFRIVQVAFIAISAALAYSYVASARDGELRKSC
                     TSLCTMAPDYAGEDRMAPDFELTTTRGSRFRLADQRGKTVLLVFWNTACDACKKQMPA
                     LRQLASTLSEEPRFQLLTVAVDESAAEVVSVLEQHTGSKDPFAVALDPDAKVVTGRYG
                     TKMYPETWLIDPAGKIRLRFDGPRDWSSPIAMDVLRGLSRDESCPMTIESMVARGPGA
                     RICQEIMP"
     gene            6059..7876
                     /locus_tag="PK710_13070"
     CDS             6059..7876
                     /locus_tag="PK710_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10701.1"
                     /translation="MTRMQAIGMGVAGALALLVMSCAADEGETSRAYTPPCGPSNCTG
                     CCNAADQCITTPSASFCGRHGAYCVSCGPGQSCSLGTCVDDVCSPSNCAGCCQNGQCV
                     SGDLESACGTGGIECSICKANESCVTGACVASSVCDANNCGNGCCIKGVCMPGRTGEA
                     CGKGGIQCEQCSEAQQCEDQVCRATVCDADSCKNGCCYQGKCMAGTSAGACGTGGVPC
                     KKCSDGEQCPKGSCVTVTCDASTCSGCCDANGNCQLGVNQAACGKGGEACVSCDANQV
                     CIQSTCTTQTQNCGPSNCSGCCNEQGKCVKGNTAAECGVSGGACTECGSGRACVDGQC
                     TCNASSCPTGCCQGDQCLSGSQQSACGKNGSSCAVCSGTDKCVNGSCSSTCGPSSCVG
                     CCQGNECKGGSSTSACGTNGQACETCVNDQQCVNGTCNSATTCNAANCNGCCKSGVCQ
                     AGTSDSQCGSGGKVCSVCKWYQYCSAKKCSFDPSSLWFVDIVEVTLEQTSYKWDVGSA
                     EESKPDLFVEFSTGSVSHTTATVWNSYTAVYNEYMFLVPASDLMTEIHYVVKDRDTVF
                     HDTVCDITEVIYQSEIENGSATIYTSCVNGMVTLKLKFY"
     gene            7928..>8162
                     /locus_tag="PK710_13075"
     CDS             7928..>8162
                     /locus_tag="PK710_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10702.1"
                     /translation="MQFTSWMRMAGLVLLAAGCCKNTSEQTPQPVPDPPVGRIDSPIV
                     NTVDTPVPPSPPPPTPAGTMTDPNRPGRGLDGLP"
BASE COUNT         1760 a         2338 c         2275 g         1789 t
ORIGIN      
        1 ccggtggctc cggtggtggc gagcccggtg gctccggtgg tggcgagccc ggtggttctg
       61 gcggtgctcc gaccggtggc tccggcggtg ctccgaccgg tggctccggc ggtggaccaa
      121 gctccgggta cggcaacctc acaaaggtca gcttcagcag cggattcatc ttcgacgagt
      181 ccaaactcaa cgatcaatcc tacttgcaag cgcacatgtc gggcatccag atgacccctg
      241 cgttcacggg ctcttatacc tcagccaacg ctgcgattcc tcccggtgga gcacaaactc
      301 tagcgttggc catccacttt gctcccaatg ggctcggtgt gttgcagcaa tcgggacaag
      361 gacaaaccgt cgtggcgccc atgatccagt ttatgttcgc gtccgatgtg gtcgcgagcg
      421 ggcccgttga tgtcggcctc gagcagacct ctggcgccat gttgctcgtc atggatccag
      481 tcggtgccaa cgatgcctgc gtggctgcca ttggtatcgg tcaggtccag gtgacccagg
      541 cccagaacac gaacgctgcc gatggcggcc agctagcgtt caacggctcc aatatcgctt
      601 tgtaccatcc atccgatacc ccgtacggca atattgagac tgaactggcc cagcaaatcg
      661 ccgtctgtcc gttcaagtaa tctcccctac cccctgcccc atgcattgcg aggctacgca
      721 attttgctag cctccgcgcc catgaccctt tcctgtgatt acctggtcat cggcagcggt
      781 gtggccgggc tcgcgttcgc cctcgaagcc gccaaccacg gccacgtcat cgtcgtcacc
      841 aaacaacgcg ccgatgaatc caacacccga tatgcccagg gagggatcgc aagcgtatta
      901 tcccaaaaag atagcttcgc ttctcatatg caagacacgc tgacggcagg cgctggtctc
      961 tgccatcccc ttgccgttca ggtatgtgta gaagaagggc ccgcgcgcat cgactggctc
     1021 gtccaacaag gggttgaatt ttctacgaaa ccaaacggta cgcaaaaaac ggacctcgat
     1081 cttgggcggg agggcggcca ttccgagcga cgcgtagccc atgcgggcga catgacgggc
     1141 aaagagatcg ttcgtgtcct cgatgcagcg gctcgtgccc accccaatat ccagttgctc
     1201 gaacaccaca ccgcggtcga cctcatcatg ctaccggaat atggggggcc cgatatctgc
     1261 ggtggagcgt atgtcctcga tgcttccagc gagcatgtcc tgacgatccg cgctcacgcc
     1321 accatgcttt gcaccggggg aagcggaaaa gtctatcttt atacctcgaa tcccgatgtc
     1381 gccacgggag acggcgtggc tatggcttat cgtgcgggtg ccgagatcgc gaacatggaa
     1441 ttttatcaat tccatccaac gtgcttgttc cattcccagg ccaagagctt tctcatcagc
     1501 gaagcgttgc gaggagaagg cgcgatcctt cgtctgctgg acggaacccc attcatgaaa
     1561 aagcatcatg aacgcgcgga cttggcccct cgtgacatcg tcgcccgttc catcgactac
     1621 gaaatgaaac gaaccggttc ggactacgtt ttgctcgaca tcacccaccg cccagcctct
     1681 ttcgttcggg aacgtttccc cggaatccat gcgacttgcc agcagtgggg catcgacatc
     1741 acctcgactc cgattcccgt ggttccagca gctcattata tgtgcggcgg cattaccacc
     1801 gacatgcatg ggcgtacgac cattccgggc ctttgggctt tgggggaagt ggcatgcacg
     1861 ggtcttcacg gtgccaatcg acttgcctcc aactccctgc tcgaagggct ggtgttggcc
     1921 catcgcgcaa gcctcaaact cagcagtcaa atcgatgcgt tgcggtcgcg cccccacgtg
     1981 acggtcaaag attgggaagt gggtgatgcc gtgtccagtg acgaaaaggt cattgtatcg
     2041 cagaactggg atgagttgcg acgcttcatg tggaattacg tcgggatcgt tcgatcggca
     2101 acccgattgc agcgcgcggc acgcaggatt gcattgctgc aagacgaaat ccacgagtac
     2161 tactggcgtt acctggtgac ccgagatctc atcgagctac gaaacatcgc caccgtggcg
     2221 gaactcatcg tggcatccgc cgcatcacgg caagaaagcc gcgggttgca cttcaccctc
     2281 gactatccgg ataaggatga tcgcttcctc gatgatacgg tgatgaagcg tggcgtctcc
     2341 gcgcggttgc gtggagtgtg aaagccagcg ctgaacagca accgcttatt tggcggatgc
     2401 cccccgttgc ggataggctg aagccatggc gttgtggtgt ttgtgtattg tatcgatgct
     2461 gcttgcttgc tgctccgcgc ctgctcgctc cggggctagt tctccaactt ctctgaacgc
     2521 acgttcgccc gagacgacga cttccccaca agcatcctct cccgccgcgt ccacgacatc
     2581 cgggcccacg gagcccatgc catcagagcc ttcggtggtc ctttcgccgg aacagccaga
     2641 ttccccaaat gaaacgatgg ctatcgctgc gtcaccctac gaagacacgc gtattctcgt
     2701 gctcatgtat cacgctttcg atcgagggaa agaaccgctc acggtcgatg gctggcagtt
     2761 cgaacggcaa ttgctttggc tgcgcgacaa cggcatcgcg atcgtgccaa cctctgccct
     2821 ggcaatgttt ttacgaaagg aaaaacctct tccgaaacgc gccgcggtca tcaccatcga
     2881 tgatggggac aagagcgtgc atgcggtcgc atggccgatt ctaaaaaaac tagaggttcc
     2941 ttttaccctc gcgctgccca ccaagctcat gaatgaaaac aagaagcatc gaatgctgac
     3001 gtgggaccaa gttcgagaaa tggtggactc cgggctgtgc gaagtcgcaa gccacggaca
     3061 cgagcacaaa agcatcgtcc acttctccaa cgaacaagct gtccgacagc tcgaaacctc
     3121 gagggatatt ctagaaaaag aaacgggcca gcgccctatc gcttttgtgt atccgttggg
     3181 agcattcgac gctcgcacga gcgcgctcgt ggaaaaagtg ggatacagta tggcgttcaa
     3241 agcctctggt gctcccatcg ggatggactg ttgcgatcct tatgcgcttc cccgcatggg
     3301 catcgttcat caagacaccg tctggtcgct tggttggtac tttagtccaa gttttctcgg
     3361 acgtatgacg cgcttgcctc gaggccgttg aagcgcttgc ccttcgtgat tgaaacgcga
     3421 tgcgccgtat cttacggacc caccagcacc gtttgttcgt ctcccacgat gataaatgac
     3481 tccggagcgt tctggttacg atagctcgcg gcgatccaag cagcacttcg cgccacgttg
     3541 ctgatggata cttcgtcgat atcacccttc caataattcg tcagcagtgt ccccatgggg
     3601 cttccaatcc aaagactatg tcccgttcca tcttcgccag tggtatagtc ccccttaccg
     3661 ttctgcaccc cgtccacata aagtatccac cgttcgttga gaccgacgac atctttttgt
     3721 tgagtccaga ccacgtatct ccattggttg tcgttgacgg gtttcgtgga actcaaccac
     3781 actccgaagc cattggtgac gacaacaacg ttgtcgagca tcccaaacgc tttgctctcc
     3841 ggtggccatt gcgcggcagg agatttggtt accagcccag cacgttgatg cgccttggtt
     3901 cgtatccagg ccgatatcgt aacatttgaa tttcccaggg aaattccatc ggctttcacg
     3961 atctggatgt gctgattttg cccgtcgaaa cgctgcgcgc gtcccacttt tcccgcaacg
     4021 gcatgattac cagctttggc tccgtgaaaa cgatcggcag ccgaattgac atgcgatgtc
     4081 gcggcatctt cttcgtccag atgccatacc gcggaaaaac catctgtcca aaccgggata
     4141 gaaggcgctg gtgaaggcgg cgacgggttg ccgtaataca tgagtatctt ggtatcgaat
     4201 aacgcgctaa gggcaggcag gcgtacccac gccacgagtg ccccattttc cgagttccac
     4261 gacacgattt cgtgagcaag tacggtcgta ctgcccccca gggtgaaacg aaagtccgca
     4321 gatgacgtga cgtgggcggc aagcgacggg tccaccacgg ttacgagtaa ggggaagccc
     4381 gacaaggttt catcgacgag cttgttcgga acggtgatga ggcgtcgaaa gcgccattga
     4441 agatcgcccc aatccgctga gggaacgagg cactggctct tgttcagggt gcagtcattt
     4501 ttgcaggaca gcactccgga tgcgtagtgg aaggtctcgc atgtcgctgc tcccagctcg
     4561 ttcccatcgc attcttctgg cccttcgatg atcccatcgc cacaagtatg gcagcgtgtc
     4621 gtatcgaacg tgcaatcgtc agagcatctc acttcgccgc tttgtcgacc cacgacagtt
     4681 tcacacgtgg cggtgcttgg cacaatcacg tcgcattgtt cgtttccttc gcgaacgcca
     4741 ttgaggcact tggagcagtt cgtaagatcc cagcgacatt gggaagtgca acgcaattcg
     4801 ccgtctgcaa ggccgatcac ggaagcacag gtttgcccgg ccaggtcgtt tccgtcacat
     4861 tgttcatttc cttcgcgtat tccatcgcca caacgaccgc atctggacac atcgaatgtg
     4921 catgtttttg agcagcgaag ttctcccccg gaatatgcca gcgtcatgca agtcatatcg
     4981 gcaacaagcg agccatcgca ttcttcgcct tcctcgagga tttcgttgcc acacgacacc
     5041 gaagacgttc cgcgaccttc cgcatcgaga acacaagcac cgaaaatgat tccaatcagc
     5101 ggtagcgata atcggaaagc aatggtccga gcggctgact tattgcgcaa cgacacgacg
     5161 gctcatctta tcatcttgct gggcagaatg cacaacgact ccatgggttg accgaacgga
     5221 gccttcccac caagattggg tcgtgatggg tgtatttttc caatctgcca cgagcaacgc
     5281 gtttcggatc gttcaagtcg cattcatagc gatatccgct gctcttgcgt attcctatgt
     5341 ggcttccgcg cgtgacggcg aattgcgcaa gtcctgcacg agtctgtgta cgatggcccc
     5401 tgactacgcg ggagaagacc gaatggcccc ggacttcgag ttgaccacaa cccgaggttc
     5461 tcgttttcgc ctcgcggatc aacggggaaa aaccgtcttg ctggtgtttt ggaacacggc
     5521 ttgcgatgcc tgcaaaaagc agatgccagc cctgcgacag ctcgcatcga ccctgagcga
     5581 agaaccacga ttccagcttc ttaccgtagc cgtcgatgag tcggcagccg aggtggtctc
     5641 tgtcctcgag cagcatacag gcagtaagga cccttttgcc gtcgccttgg atccggatgc
     5701 caaggtggtg acaggacgtt atggaacgaa gatgtatcct gaaacgtggc tgattgatcc
     5761 agccggtaaa attcgcttgc gtttcgatgg accgagagac tggtcgagtc cgatcgccat
     5821 ggatgtgctt cgtggtttgt cacgtgatga gtcgtgtccc atgacgatcg aatcgatggt
     5881 ggcccggggt cctggcgcga ggatctgcca agagatcatg ccgtgattct gtggcaatcg
     5941 gcctagtttt cacgaaggga agcgagaaga actgccctct gtcgcgatcg cgaatcgtca
     6001 ccgtttcagc gcttaccaaa ggataaaagg ggtggtatgc cttgatggga ggcatgccat
     6061 gacacgaatg caagcaatcg gaatgggagt cgcgggggct ttggctctgc tcgtgatgag
     6121 ttgcgcagcg gatgaaggcg agacatctcg cgcgtacacg ccgccttgtg gaccttcgaa
     6181 ctgcaccggc tgttgtaatg ctgccgacca atgcattacg acaccttccg cttcgttttg
     6241 cgggcgccac ggagcttatt gtgtttcttg tggacctggg cagtcctgct ctttgggcac
     6301 gtgtgtcgac gacgtatgca gcccgtccaa ttgtgcgggg tgttgtcaaa acggacaatg
     6361 tgtgagtgga gacttggaaa gtgcatgtgg aacgggcggg atcgaatgtt cgatttgcaa
     6421 agcgaacgaa agctgtgtga cgggtgcttg cgttgcgagc agcgtatgtg acgccaacaa
     6481 ctgtggaaac ggttgctgta tcaaaggtgt atgcatgccg ggccgcacgg gggaggcttg
     6541 tggaaagggg ggaatccagt gtgagcaatg ctcagaggcg caacaatgtg aggatcaggt
     6601 ttgtcgagcc acggtttgcg acgcggattc ttgcaaaaac ggttgttgtt atcagggcaa
     6661 gtgtatggct ggaacgagtg ccggagcatg cggaacaggg ggtgttcctt gtaagaaatg
     6721 cagcgacggt gagcaatgcc cgaaaggttc ttgcgtcacg gtgacatgcg atgcttcgac
     6781 gtgttcgggg tgttgcgatg cgaacggcaa ttgtcagctt ggcgtcaacc aagccgcctg
     6841 tggaaaaggc ggagaagctt gtgtttcctg cgatgccaac caggtttgta tccaatcgac
     6901 atgcacgacc cagacacaga attgtggacc ttcgaattgt tcaggatgct gcaacgaaca
     6961 gggtaagtgc gtgaagggaa atacggcagc ggaatgcgga gtgagtggcg gtgcctgcac
     7021 ggagtgtggc tcgggccgag catgtgtcga tggccaatgt acgtgcaatg catcctcttg
     7081 ccccacgggc tgctgtcaag gcgaccaatg cctgtccggc tcgcaacaat ccgcatgcgg
     7141 aaaaaatggt tcctcctgcg ccgtttgctc aggaacggac aaatgcgtca acggctcttg
     7201 cagttcaacc tgtggtcctt ccagttgcgt gggctgttgc caagggaatg aatgcaaagg
     7261 gggaagctca acctccgcct gtggaaccaa tggtcaagcc tgcgaaacct gcgtcaatga
     7321 ccagcagtgc gtaaacggca catgcaacag tgccaccacg tgcaatgccg ccaactgcaa
     7381 cggatgctgc aagtccggcg tgtgtcaagc cggaacctcg gacagccaat gcggttctgg
     7441 cggcaaagtc tgttccgtct gcaagtggta ccaatattgt tccgccaaga aatgctcttt
     7501 cgacccctcc tcgctgtggt tcgtcgatat cgtcgaagtg accctcgaac aaacctccta
     7561 caaatgggac gtaggctccg ccgaggaatc caaacccgac ctattcgtgg aattcagcac
     7621 tggctcggtt tcccacacca ccgccaccgt ttggaattcc tatacggccg tttacaacga
     7681 gtacatgttt ctcgtccccg cttcggattt aatgaccgaa atccattatg tcgtaaagga
     7741 ccgagacacg gtctttcacg acaccgtatg cgacatcacg gaggtcatct atcaatccga
     7801 aatcgaaaac ggctcggcca cgatctacac gtcttgtgtc aatggcatgg tgaccttgaa
     7861 actcaaattc tattgaatcg cagttctgcc gtgacaaaac gcggtgtcca aacgtagtct
     7921 gaagaccatg caattcactt cctggatgcg aatggcaggg ctcgtattgc tcgcagcggg
     7981 ctgctgcaag aacacatcgg aacaaacacc acaacctgta ccggatccgc ccgtaggcag
     8041 gatagacagc ccgatcgtca acacggtcga cacacccgtt cctccctcgc ctcccccgcc
     8101 caccccggca ggtacgatga ccgatcccaa tcgtccggga aggggtctcg atggtctccc
     8161 cg
//
LOCUS       DAOSOR010000228         8140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8801_length_8140_cov_3.845269, whole genome shotgun sequence.
ACCESSION   DAOSOR010000228 DAOSOR010000000
VERSION     DAOSOR010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8140
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8801_length_8140_cov_3.845269"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PK710_13080"
     CDS             <1..314
                     /locus_tag="PK710_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10703.1"
                     /translation="TGKPVGDDLRRGDKTAIAQQAFRLLNPEQREAFDAVFGCPEATD
                     EQIAQVTEMLTEQGVRGAVVERIHDLIHEAISALVGAPIDPAPRSMLLGFCSILASRI
                     D"
     gene            334..1224
                     /gene="mvk"
                     /locus_tag="PK710_13085"
     CDS             334..1224
                     /gene="mvk"
                     /locus_tag="PK710_13085"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HOT10704.1"
                     /translation="MAGEAKASGKAILLGEHAVVHGVPALAISIGQGARASARLAPTA
                     ALQVRDKAGSWEIEKDSDLDRAFHALVKEVGVIADVHAHVDIPTRAGLGSSACLGVAL
                     ARALLTCRGEPFDAPRVMQAAMVWERVFHGNPSGVDVAVAVQGGCIEYSRSTGATPVP
                     LAQPIPLCIGDTGDRSCTRQMVQRVSLVLDRQFDGGRHGLEAIRSCVETARKALVTAD
                     WQGVANAMHDNQQWLVDLGLNTPNSDKLCEIALAAGALAAKITGAGGGGCVIALAPRR
                     QQEVLDAWRASGFEAFAIHP"
     gene            complement(1235..4297)
                     /locus_tag="PK710_13090"
     CDS             complement(1235..4297)
                     /locus_tag="PK710_13090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HOT10705.1"
                     /translation="MPPFAELCARSCFSFLRGASHPEEMVEQAYKLGLHAIGLCDTDG
                     LYGLVRAHQATQKHPIKLIVGASLHLEDHPGQLCLLARTRKGYQNLCRLLTIAHANKP
                     KGGARLDPTTLPAHAEGLFAILVPPQQAALCQNVRQAFAPEHVRLAVERQMTPDQSAQ
                     ESLAVSLAEQFRLRIVATGQPLFHCASRKPVADVLCCIREKTTLDEAGTRLLPNTEAR
                     LSSPERMTRLFADHPEWVEESVHVADGCTFSLSELQYRFPFDHVVRPGESPDEALRRL
                     VRERLGWRYPQGAAASVLTQIEKELSLIQQLEAAPYFLSVYEIVEIARQKQILCQGRG
                     SAANSAVCFVLGITAVDPARSNLLFERFMSLERKDPPDIDVDFEHERREEVIQEIYRR
                     YGRERAAMVAEVISYRAKSALRDVGKAFGLSLEQVDRLSGIVAHGWESIEQGEQRLHE
                     SGFDPRDPRIASVIKISQQIQGFPRHLSIHVGGFILSSCSLHEVAPIEPARMPGRTVI
                     PWDKDDLEALGFFKVDILALGMLTCIRKALAVVMPDRDPFTALASIPAEDPAVYEMFG
                     RADTVGVFQIESRAQMAMLPRLRPQRFYDLVIEVAIVRPGPIQGGMVHPYLARRSGKV
                     RVDCPHPCLGPILDRTLGVPLFQEQVMQIAIVGAGYSGGEADQLRRDMAAWRKHGRLL
                     EHRERLLRGFEKTGISKRFGERLFKQIQGFGEYGFPESHAASFALLVYASGWLKAHHP
                     AAFCAALINSQPMGFYSPATIVRDARGHGVQVLPIRVNASDWDCKLEHGHPANVRLGF
                     RLLRGLGKAAGSSIENARIKGPFLDFNDFLLRTALRKDAVEILAEAGAFEDLVPERRQ
                     ALWKARAPRSGSLFAHEPIREPDVALPALRPVEQLVLDYGRVGFSLQDHPMRHLRSKL
                     EQTLFAEQLQHMRHGSKVAVAGVVINRQRPSTASGVVFMTLEDETAMISLVLWPSVFE
                     AYRHILTHSKILWVQGTLDRREGVTHILVRAARRLDSPVGRIQSRDFR"
     gene            complement(4339..5253)
                     /locus_tag="PK710_13095"
     CDS             complement(4339..5253)
                     /locus_tag="PK710_13095"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT10706.1"
                     /translation="MSAVFHRSIQPLTRHLGGRHGQIPAHPLSSPDSYSVAARDLSFH
                     ILEWTGSDPPLVFFHGLNNNAWSWARVASLFAPKRRILALDSRGHGHTAAPASGYSLE
                     DTTQDIKAVFDTLGLDTVDLAGHSWGGKVAMHFTCTHPTRVHRLALADPVPPAGFNRL
                     LQAFPGLISLALRAERGPFPNHQAWEKAGKRLVYLQPWDAWDQKLWSAAFRQTAHGAY
                     HHVLPESAFQEIILHALQQDITDKLGALRMPVLLMRPTLTLSFFPGELRSFRKALPHR
                     IEKRIAGDHTFIHTNPFDTHDILQKFFS"
     gene            complement(5346..6887)
                     /locus_tag="PK710_13100"
     CDS             complement(5346..6887)
                     /locus_tag="PK710_13100"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HOT10707.1"
                     /translation="MPPRRITAIVLPHLLCELASAMAACRDPFAVILQDSAASQPLRA
                     RTRLDAVSDAAHRAGIRAGQSLAEAQAHLPCLSVLQLSPLHVQQTLARIAETALRFCP
                     HVEISYPDALYLDMTGATHLYGSESSALHEVRTTVERLGHVARVAMAQGPRIAEMVAR
                     QGPHNEQVVAPFEERDQIQGFAISALPIDPAIAQWFTRVGVITLGDLLRLPRAQVTAR
                     LGDKAPELLDLAAGHDPRPLHAYTPAAVLCEQAAWEQGLTDLSALVFALHRLVQNMTH
                     RLQGRGQALMGLRLILEHDPAIARFQEAAPESSTQVTLPVPIDRAEPLLRILRARLET
                     LSLPAPVVGLRLETSQLVAAPRIQLTLSHQSSAPADALALLVAELSADLGMERVGTLA
                     LVDSYRPETRSCLHPPPLPSFASPPSLPSFSFAQVTQILPKPIEITKQILRPGLSIVL
                     DSQSLRIEQMQFHQRLSGIEWWGPSSFNRDYYMIWLQNASTGGRGWFFRDRIRDVYYL
                     HGWFD"
     gene            complement(7286..>8140)
                     /locus_tag="PK710_13105"
     CDS             complement(7286..>8140)
                     /locus_tag="PK710_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10708.1"
                     /translation="APYKARTVTFHLPDFIDIVVNAGDSRHPLGATIGQSLPNWGPVA
                     NEGRGRTVVMSNLYTDPDSQLIRKEQAESMLDAATAAKRSSAQDPSLLSTILHEATHN
                     FGPSHEYKVQGKTDDQHFGGPLASTMEELKAQTGALWYIAMLEKRKIIEPELAQQTYV
                     DSLIWALNHIARGMYTDDKKPKTYSHVSAIQVGFLMQEGALTFDPAAPAVNGKDKGAF
                     TLHFDKLPAAIDKMMKVVGGIKAKGDRKQAEELVKKYVDGDMVPMAIIAERSLRHPKA
                     AFVYGLAL"
BASE COUNT         1857 a         2309 c         2350 g         1624 t
ORIGIN      
        1 agacaggaaa acccgtgggc gacgatctgc gaagaggtga caaaaccgcg attgcccagc
       61 aggctttccg attgctcaac cccgagcaac gagaagcttt cgatgccgtt tttggatgcc
      121 ccgaggctac cgacgaacag atcgctcagg tgaccgaaat gctcacggaa cagggagtcc
      181 gcggagccgt cgtcgagcga atccatgatt tgattcatga agccatttcc gcgcttgtgg
      241 gagcccccat cgaccccgcc ccccgttcca tgctccttgg cttctgttca atccttgctt
      301 cccgcatcga ttgagccatc ccggtgtacg agtatggctg gtgaagcgaa agcaagcgga
      361 aaagcgattt tgcttggtga acacgcggta gtccatggcg tccccgcgtt agccatcagc
      421 atcggacaag gcgcccgagc ctcggctcgc ctggctccta cggcggctct gcaggtgcga
      481 gataaggctg gatcgtggga aatcgaaaag gactccgacc tggatcgagc gtttcatgct
      541 ctggtgaaag aggtgggcgt gatcgccgac gttcatgccc atgtcgatat cccaacccgc
      601 gccggactcg gctcatccgc ttgccttggc gttgctctgg ctcgcgccct tctgacctgc
      661 cgaggcgaac cgttcgatgc cccgcgagtc atgcaagcag ccatggtgtg ggagcgcgtt
      721 tttcacggaa atccttcggg ggtcgatgtg gccgtggccg ttcaaggcgg gtgcatcgag
      781 tactcccgct ccacgggagc aaccccggtg ccgcttgccc aacccatccc cctttgcatc
      841 ggcgatacgg gcgatcgctc ttgcactcga cagatggtgc aacgcgtgag ccttgtgctc
      901 gatcgacaat tcgatggtgg ccggcatgga ctcgaagcca tccgatcatg cgttgaaacc
      961 gccagaaaag cgcttgtcac cgccgattgg caaggcgtgg ccaacgccat gcatgacaac
     1021 caacaatggc tcgtcgacct ggggctgaac acgccaaata gcgataagct ctgcgaaata
     1081 gcccttgccg ccggcgccct cgccgcgaaa atcacagggg ctggaggcgg tggttgcgtc
     1141 atcgccctcg caccacgtcg acagcaagag gttctcgacg cctggcgcgc ttcaggcttc
     1201 gaggcttttg ccatccatcc atgatcgttt cccatcaacg aaaatctcgg gattgaattc
     1261 tgcctaccgg tgaatccaaa cgccgcgcgg cgcgtacgag gatatgcgtg actccctcgc
     1321 gcctgtccaa cgttccttgc acccacaaaa tcttgctgtg cgtcaagata tgccgatacg
     1381 cttcgaatac agaaggccaa agcacaagac tgatcatcgc cgtttcatcc tcgagcgtca
     1441 tgaaaacaac cccgctcgcc gtcgatggac gctgccgatt gatcaccaca cccgccacgg
     1501 caaccttgga gccgtgcctc atgtgctgga gttgttcggc aaacaaggtt tgctccagct
     1561 tcgaacgcaa atgccgcatg gggtgatcct gcagcgaaaa acccacgcgc ccgtaatcca
     1621 agaccagttg ttcgacagga cgaagggctg gcaacgctac gtctggttct cgtatcggct
     1681 catgggcaaa aagtgaacct gaccgcgggg ctcgggcttt ccacaaagct tgacgacgct
     1741 ccggcacgag gtcttcgaag gcgcctgctt ccgccaaaat ctcgacggca tccttgcgca
     1801 atgccgtacg tagtaaaaaa tcgttaaaat ccaggaaagg gcctttgatc cgtgcatttt
     1861 caatgctcga acccgccgct ttgccaaggc cccgaaggag acgaaacccg agccgcacgt
     1921 tggcaggatg gccatgctcg agcttgcaat cccaatcgct ggcgttcaca cggatcggca
     1981 gcacttgcac cccatgtcct cgggcatcgc gcacgatcgt cgcgggacta tagaatccca
     2041 tgggctggct gttgatcaaa gcggcgcaaa acgccgccgg atggtgagct ttgagccagc
     2101 cggaagcata aacgagcaag gcaaaagagg ccgcatggga ttcgggaaat ccgtactccc
     2161 caaatccttg gatctgtttg aaaagacgtt ccccaaagcg cttcgagatc cctgtttttt
     2221 caaaaccccg gagcaatcgt tcccgatgtt ccagcaaccg accatgcttc ctccacgctg
     2281 ccatatccct gcgcaattga tcggcctcgc cccccgaata ccccgccccc acgatcgcga
     2341 tctgcatgac ttgctcttga aatagcggta ccccaagcgt tctatccaaa atcggaccaa
     2401 gacaaggatg aggacaatcg acccggacct tgccgcttct gcgcgcaaga tacggatgca
     2461 ccatgccccc ttgaatcggc cccggacgaa cgatcgccac ctcgatgacg agatcataaa
     2521 accgttgggg ccgaagccgc ggcaacatgg ccatctgcgc ccgactctca atctgaaaaa
     2581 cacccaccgt gtcggcccga ccaaacatct cataaacggc tggatcctca gcgggaatcg
     2641 aggccagggc cgtgaaagga tcccggtcgg gcatcaccac cgccagcgct ttgcggatac
     2701 acgtgagcat cccaagagcc aaaatatcga ctttgaaaaa tccaagagcc tccagatcat
     2761 ccttgtccca tgggatcacc gtgcgcccgg gcatgcgagc aggctcgatc ggagccacct
     2821 catgcaaaga acaggaagac aagatgaatc caccgacatg gatcgataga tgcctcggaa
     2881 aaccttgaat ttgttgtgaa atttttatga ccgaggcgat cctggggtcc cgtggatcga
     2941 aacccgactc atgaagccgc tgctcgccct gctcgatcga ttcccaaccg tgggcgacga
     3001 tcccagagag ccgatccact tgttccaacg acaaaccgaa ggctttgcct acgtcccgaa
     3061 gcgcgctttt ggcccgatag ctgatcacct ccgcaaccat ggcggctcgc tctctgccat
     3121 aacgacgata aatctcttga atgacctctt cacgacgctc gtgctcgaag tccacatcga
     3181 tatcgggcgg atcctttcgc tccaaggaca tgaaccgctc gaagagcaag ttggaacgag
     3241 cagggtcgac ggcggtgatg cccagcacga aacagacggc gctgttggcc gcgctgccgc
     3301 gtccttggca aaggatctgc ttttgccttg cgatctcgac gatctcgtag acgctcaaaa
     3361 agtacggcgc tgcttccaat tgctggatga gactcagctc cttttcgatc tgagtaagta
     3421 cgctcgcagc agctccctgg ggatatcgcc atccaagccg ctcgcgcaca agcctgcgaa
     3481 gagcctcgtc cgggctttcc ccaggcctta cgacatgatc gaaagggaaa cgatactgca
     3541 attccgataa agaaaatgtg cagccgtccg ccacatggac ggattcttcc acccactcgg
     3601 gatggtctgc gaaaagacgc gtcatgcgct ccggcgagga taggcgggcc tcggtattcg
     3661 gtaataaacg cgtccctgcc tcgtcgaggg tggttttttc gcggatacaa cataaaacgt
     3721 ccgcgacggg ctttcgcgag gcacaatgaa aaagcggctg ccccgtcgcc acgatccgaa
     3781 gccgaaattg ctcggctagg ctcacggcca aagactcctg cgccgattga tccggagtca
     3841 tctgccgctc gaccgccaat cgcacgtgtt cgggagcaaa agcctgacgc acgttctgac
     3901 aaagtgcggc ttgttggggc gggacaagga ttgcgaacaa cccctcggca tgggcgggca
     3961 gggtcgtggg atccagacgc gctccccctt tgggtttgtt ggcgtgagcg atcgtgagca
     4021 gacgacaaag gttttggtat cctttgcgcg ttcgtgcgag caggcaaagc tgtccggggt
     4081 gatcttcgag atgcagagag gcgcccacga tgagtttgat ggggtgcttt tgggtggcct
     4141 ggtgagcccg aacgagaccg taaagaccat cggtatcgca tagaccgatc gcgtgcaggc
     4201 cgagcttata cgcttgttcg accatttcct cgggatggga tgccccgcgc aggaacgaaa
     4261 agcaggagcg agcgcaaagt tcggcgaaag gcggcatata cctgaacata atagcaggtg
     4321 ttgggggagc aagacggatt aggagaaaaa tttctgcaaa atatcgtggg tatcgaaggg
     4381 attggtgtga atgaaggtat ggtctccggc gattcttttc tcgatccgat gcggcaacgc
     4441 cttgcgaaaa gaacgcaatt cgcccgggaa gaaggataag gtgagggtgg gacgcatcag
     4501 aagcactggc attcgaaggg ctccgagctt atccgtgatg tcttgctgca aagcgtggag
     4561 gataatttct tggaatgccg attcaggcaa tacgtggtgg tacgccccat gggcggtttg
     4621 gcgaaaggcc gcggaccaaa gcttttgatc ccacgcatcc caaggctgca agtaaacgag
     4681 gcgttttccg gccttttccc atgcctgatg attcggaaac ggcccccgct cggctcgcaa
     4741 ggcaagagaa atcaatccgg gaaaagcctg aaggagccgg ttgaacccgg caggcggcac
     4801 cggatccgcc agagcaagac gatgcacccg ggtgggatgc gtgcaggtaa agtgcattgc
     4861 cactttgcct ccccaggaat gccccgccag gtccaccgta tcgagcccta acgtatcgaa
     4921 aaccgctttg atatcttggg tagtatcttc caacgaatat cccgaggcag gggccgcagt
     4981 atggccgtga ccccgtgaat cgagagccaa tatccttcgt tttggcgcaa aaaggctagc
     5041 cacgcgcgcc cacgaccagg cattgttgtt caaaccatga aaaaacacca agggtggatc
     5101 cgatccggtc cattccaaaa tatggaaaga tagatctcgt gccgctacgg aataggagtc
     5161 gggcgaagaa aggggatggg cggggatttg cccatgacga ccgcctagat gccgcgtgag
     5221 cggttggatc gaacgatgaa acacagcgga catgccgagg atcgacatga ccgacggaat
     5281 agcatgaaat aaaacggatg acaggtccat ccggaggcgg ctttggcttg cctcgtcgtg
     5341 accgctcaat cgaaccaacc gtgtaaataa taaacgtcgc ggatccggtc gcgaaaaaac
     5401 caaccgcggc cccccgtcga agcgttttgt aaccaaatca tataataatc ccggttaaag
     5461 gaagaaggac cccaccattc gatgccggaa agacgctgat gaaactgcat ctgctcgatg
     5521 cgcaacgatt gggaatcaag cacgatcgaa agcccggggc gtaggatttg ttttgtgatt
     5581 tcaatcggtt ttggaaggat ttgggtcacc tgcgcaaaag aaaaagaggg taaagaaggt
     5641 ggagacgcaa acgatggcag gggaggggga tgcagacaag atcgcgtttc aggccggtag
     5701 gaatcgacca gcgcaagcgt tcccacccgc tccatgccca agtccgcgga aagctcagcc
     5761 accaataggg caagagcatc ggcaggggcc gagctttggt gcgatagtgt gagctggatg
     5821 cgtggcgcgg cgacgagttg gctcgtttcg agccgaagtc ccacgacggg cgccggcagc
     5881 gataaggttt cgagccgggc ccggaggatg cgaagcaaag gctcggcgcg atcgataggc
     5941 accggcaacg tcacctgtgt gctcgattca ggcgcagctt cttggaatcg agcgatggcg
     6001 gggtcgtgct cgagaatgag gcgtagtccc atcagggctt gccctctgcc ttgtaatcga
     6061 tgggtcatgt tctgcaccag acgatgcagg gcgaagacca gcgccgacag atccgtaagg
     6121 ccttgctccc acgcggcttg ctcgcaaagg acagcggcag gggtataagc gtgcagggga
     6181 cgagggtcgt ggcccgcggc taagtccaac agttccgggg ctttgtcgcc gagtcttgcc
     6241 gtgacctggg cccgcggcag ccggagcaga tcgccaaggg tgatgactcc gacccgcgtg
     6301 aaccattgag cgatagccgg atcgatgggc aaggcggaaa tggcaaagcc ctggatctga
     6361 tcccgttctt cgaaaggcgc taccacttgt tcgttgtggg ggccctgacg cgccaccatc
     6421 tcggcgatgc gaggtccctg cgccatcgcc acccgcgcga catgccccaa tcgctccacc
     6481 gtggttctca cctcgtgaag ggcagatgat tcgctaccat acaaatgggt ggcccccgtc
     6541 atatcgagat aaagcgcatc tggataagag atttcgacgt gagggcagaa gcggagcgcg
     6601 gtttcggcga tgcgggccag agtctgttgc acgtgcagag gggaaagctg aagaaccgac
     6661 aggcaaggca aatgggcttg ggcttccgca agactttgcc ctgcgcgtat tcccgctcgg
     6721 tgggcagcat cgcttaccgc atccaaccgt gtccgggctc gcaagggctg ggaagcggca
     6781 gaatcctgca gtatcaccgc gaaaggatcg cgacatgcgg ccatggcgga ggccagttca
     6841 caaagcaaat gaggcaagac gatggcagtg atacgtcggg ggggcatact gaacatgcta
     6901 tcaggttttg caaaggatgc caccagggaa aaatggttct tggcgttgat ggtgttgcca
     6961 aaagacagcg tgcggtcggg ctgtcgcggg cttttggata cggcctattt gccgaacctg
     7021 attcacgcaa cgttttgtcg aaaaacaata taatgttgcg gcaacgtatt atgatttctg
     7081 gatggtgtga acgcagatat tattgcaatt tataggggaa aacatataac gttgcttctt
     7141 caaatttcaa agattgctct tttgggcgaa gcagttctga tggaaggcag gccgggcttt
     7201 tgggcccggc cttggaggtt gtgggaggga atggatgaaa ttgtagacgc aacaacttgt
     7261 gttgcgcgac gagatgtctg cgattctaca gggcaaggcc gtagacgaag gccgctttcg
     7321 gatgccgcaa ggagcgctcg gcaatgatcg ccatcggaac catatcgcca tccacgtatt
     7381 ttttgacgag ttcctcggct tgcttccgat ctcctttggc tttgatcccc cctaccactt
     7441 tcatcatctt gtcgatggcc gcggggagtt tatcgaaatg caaggtaaag gctcccttgt
     7501 ctttgccgtt gaccgcgggt gccgcaggat cgaacgtcaa ggccccctcc tgcatgagaa
     7561 aacccacttg aatcgccgat acatggctgt atgtcttcgg ctttttgtcg tcggtataca
     7621 tcccccgggc aatatggttc aacgcccaga tcagcgaatc gacataggtt tgctgcgcca
     7681 gctccggttc gatgatcttg cgcttttcga gcatcgcaat gtaccacaag gctcccgttt
     7741 gggctttcag ctcctccatc gtgctcgcca acggaccccc aaaatgctga tcatcggttt
     7801 tgccctgaac cttatactcg tgcgatggcc cgaaattatg ggtggcttca tggagaatcg
     7861 tcgacaacaa agaaggatcc tgggcggaag aacgcttggc cgccgtggcc gcatccagca
     7921 tcgactccgc ttgctcttta cgaatgagtt ggctgtccgg atccgtgtat agattgctca
     7981 tcacgactgt ccgaccgcgc ccttcattgg ccacgggccc ccaattggga agactctgcc
     8041 cgatggtcgc tccaagagga tgcctggaat caccagcatt gacgacgata tcgatgaaat
     8101 ccggcaaatg aaacgtaacc gttcgcgcct tgtacggggc
//
LOCUS       DAOSOR010000229         8114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8834_length_8114_cov_4.772304, whole genome shotgun sequence.
ACCESSION   DAOSOR010000229 DAOSOR010000000
VERSION     DAOSOR010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8114
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8834_length_8114_cov_4.772304"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PK710_13110"
     CDS             complement(<1..689)
                     /locus_tag="PK710_13110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT10709.1"
                     /translation="MHVLVVDDDPEFRELIRRMLEPFHAVREAQQGLEALRSMENDRP
                     TLVLLDMSLSDVDGVHVLRQMQLDPDLADIPVIIVSGMDDIHHKVLALEAGANDYLVK
                     PIIREELLARIAVQLRRSSGSLTSTGTQRLPSVSGASLQKNSIIDDRYIVTGVLGKGG
                     MGIVYEAIQIQLGRPVAIKVLREGTRRKVHRAKRLEREAQIVGSLGHAHICQVYDMGN
                     LEDGSPYVVME"
     gene            complement(1001..1285)
                     /locus_tag="PK710_13115"
     CDS             complement(1001..1285)
                     /locus_tag="PK710_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10710.1"
                     /translation="MNSRAFASSSKCFLVILLSGHERELAASATMPPTSKARSASTAT
                     IDAAAHDHLEDAGLIGEGGREDEGGMGGKWTQYVAILQSSFSNAWFDVRF"
     gene            1326..1571
                     /locus_tag="PK710_13120"
     CDS             1326..1571
                     /locus_tag="PK710_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10711.1"
                     /translation="MDSLEYERWTDWHLRAELQFSASAQVDEYLVQAHKHRSLRDDYC
                     GSQDPAGGARYYLPASNACGTLRRSSPMMLSVWCYTR"
     gene            complement(1768..3210)
                     /locus_tag="PK710_13125"
     CDS             complement(1768..3210)
                     /locus_tag="PK710_13125"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOT10712.1"
                     /translation="MYRQWVMGSMVGIFSFGWAACGSDDADPVVAEDRDGASLTDSAT
                     VPDGSSVADSSTPPDGSSPPDSSTPPNGCLDPNAINVADYGAKANAHFFDPSTKKYYQ
                     DANHTVPSEDATQAINEAIQDASKKGIVNVCIPDGTYLIKAEAASATHQFEYSTHGGI
                     QLQDNVTLTMSSGVKLVMNTGSQGGYAFISIDHKKNVKVIGGRIEGDKDTHPPDKHNY
                     CYGINIANGSSDVLIEDVDISNCEDDGIMIADYLEPGYGGGITDHVRIHKVKMHHNGR
                     QGLSITSGTNISVTECELSHQTKHDPKSGIDIELENFEFRGVKNVTIARNTFEGNTFA
                     AIVVSDMRNELPQSLSENIVITENTIAGDRWGVVVPGWVKGLEISNNTVTIDPVNYPC
                     SAGITTSAPDSDSVLIRGNHITSTHKECTIGILLWEGNNHVDVLENHIIKPYEGIVVV
                     DTPATVKDNVIEGTVVPISGAPQDGNTIIP"
     gene            3830..4549
                     /locus_tag="PK710_13130"
     CDS             3830..4549
                     /locus_tag="PK710_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10713.1"
                     /translation="MMHPISIPFHRIPVAFLALLLTAGCGDEKTSSDTTPHDSGMKEN
                     DAQTGGQGGSAGTAAEGGAGGSDCSLQDKEAQTQSARNALREQVPCFSGSFAVDKNGG
                     IHLSFFLSTDFNGPRALLCHTDSNVSVSKAHEQAASKALEGLDLSCLKGLYGIDIAGG
                     ACEQRDTLEELLAACPPWWEHVNEELGIVFDENGQAVSLKPAEGKPSEPEIETCVLDA
                     LAGKTFPCLANLEVCAGNPIP"
     gene            complement(4945..5394)
                     /locus_tag="PK710_13135"
     CDS             complement(4945..5394)
                     /locus_tag="PK710_13135"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOT10714.1"
                     /translation="MERYGVLVARTLLAVIFIISGFGKLADLSGTAAYIGSTGLPAPM
                     LLALLAGVSELVGGVMVVVGFQTRLAAIGLALFLVPATVLFHNPFGLEGAEAHMQQIH
                     ALKNIAIAGGFLALAVHGAGAISLEAWLGRRVRPVEGAPTAPPVVSG"
     gene            complement(5408..5953)
                     /locus_tag="PK710_13140"
     CDS             complement(5408..5953)
                     /locus_tag="PK710_13140"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOT10715.1"
                     /translation="MSLEKWEIDSSHSGIFFSVRHMVIAKVRGQFSRWSGNIVIEDGD
                     MARAKVEVVIDASSIDTGVADRDAHLKNPDFLDVENFPELTFQSVRVEKQGEENFRLI
                     GNLTIHGVTREVVLDAESAGQTKDPWGNVRAGYAAKTSINRKDFGLTWNQVLEAGGVM
                     VGDRITIEIEVEAVKQEAAKE"
     gene            6131..7765
                     /locus_tag="PK710_13145"
     CDS             6131..7765
                     /locus_tag="PK710_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT10716.1"
                     /translation="MKAADLDLRELLHFEPKGGVLTFAGQRALLFDAVALGLLRRELV
                     KSLGLSGARGMLTRFGYAHGWRTAETLKNAFPWDDESEWRRAGGRLHALQGMVTFELP
                     SRATNTGPEAFAEAIWHDSYEAEQHLLHLGRAEEPVCWTLTGFASGYLSYCNGREIYC
                     IEERCRGKGDAVCTLVGRTREDWGDAITPHLPFYKKECLEEALAQVATELKRVEERLR
                     ARKKVLGHDAPESTGSGVVVQSEAMLRVLDLARRIAKVDSTVLITGESGVGKERLARF
                     IHDESVRTGGPFVAINCGAMPEGLLESELFGHVKGAFTGATQDRPGLFEAANGGTLLL
                     DEIGEVSPPMQVKLLRALQEREVRRVGENKSRKVDVRLLAATNRDLLSEVHGARFRQD
                     LYYRLRVVELRVPPLRERRDDILALARTFLASASERMGRKVTSFTPGAANQLLRYDWP
                     GNVRELENAMERAVALAKTSQIDIDDLPEEVGIAIPSAYAPGDVRPLADVERDYILAV
                     VRANQGNKAAAAEQLEIGVATLYRKLKQYESVGGVS"
BASE COUNT         1819 a         2348 c         2123 g         1824 t
ORIGIN      
        1 ttttccatga cgacatacgg ggatccatct tcgagatttc ccatgtcgta gacttgacaa
       61 atatgggcgt ggccgaggga accgacgatc tgagcttctc gctccaagcg cttggctcta
      121 tggacttttc gacgagtgcc ttcgcgcaaa actttgatgg cgaccgggcg tccgagttgg
      181 atctggatag cctcgtacac gatgcccatc ccgcctttgc caagaactcc cgtcacaatg
      241 tatcggtcgt cgatgatgga gtttttttgc aggcttgcgc cactgacaga aggcaaacgt
      301 tgtgtgcctg tcgaagtcaa cgaaccagag gaacgacgaa gctgaacggc gattcgtgcg
      361 agcagttctt cccgaatgat cggtttgacc aggtaatcgt tggctcccgc ttccaaagcc
      421 agcaccttgt ggtggatatc atccatgccg ctgacgatga tcacgggaat atcggcaaga
      481 tccggatcca attgcatctg gcgcaacaca tgcacaccat cgacgtccga taggctcata
      541 tcgagcaaga ccagggtagg acggtcattt tccatgctgc gcaaggcttc tagaccttgt
      601 tgtgcttcac gcaccgcatg gaacggctcg agcatgcgcc gaatcagctc gcggaattca
      661 gggtcgtcat caaccacaag cacgtgcatg gtgttctcgg gacgcggtcc aagtcataag
      721 gcttgcacga atccgtccgt gggggaagca caatcccgtt agggtggact tacctatccg
      781 taatgggata tgaagcgcgt gcgccaacgt agcgttgaaa aatacgcgac aagagtgcga
      841 cggaagacac gacaacctgg catctcattt tcccagcttt gtcgagcgat gcgacgtgct
      901 cttccaccgt agcggtcatc cagcgagggg tcatccgcgt ctttggatcc taccttgttg
      961 tatcaagtac gtccaaagtg tacggcaccg tccatcacat tcaaaaacgc acatcgaacc
     1021 aggcattcga aaaagacgat tgcaaaatcg cgacatattg ggtccatttc ccccccatgc
     1081 ccccttcatc ctcccgccca ccttctccta tcaacccagc gtcttccagg tggtcgtggg
     1141 ctgccgcgtc gatcgtcgcc gtactagcgc ttctggcttt gctggtcggg ggcatcgtag
     1201 cgctcgctgc caactccctt tcatggcccg acaacaaaat caccaaaaag cacttcgaag
     1261 acgaggcgaa agcccgcgag ttcacctcaa tacacctccc cttccccctt ccccacgatg
     1321 tcgtcgtgga ctcactcgaa tacgagcgct ggacagactg gcacttacgc gccgagcttc
     1381 agttttcagc aagcgcgcaa gtggacgaat acctcgtaca agcccataaa caccgctctc
     1441 ttcgcgacga ctactgtggc tcccaagatc ctgccggcgg cgcccggtat tacctccctg
     1501 cctcgaacgc atgcggcacc ctccgccgct cttcaccaat gatgttatcc gtatggtgct
     1561 atacgcgctg acgcttcccg gtatcttatg gaaccatccc ctcttttcct gttggcagat
     1621 ccttcgtcaa cgataggcac aaggcgagat ttgggctgga gataaggcca ggttcgtcaa
     1681 aatcatcgtg atcgccaact tctcggtgcc atagtacgga aacgtcacac caacccgccg
     1741 cgggaaacct tcgtaaggca gaaataacta tggaataatg gtgttgccat cctgaggcgc
     1801 accgctgatg ggcacgacgg taccttcaat cacgttgtcc ttgaccgtcg cgggcgtgtc
     1861 taccaccaca atcccttcgt agggcttaat aatgtgattc tcaaggacat ccacgtggtt
     1921 atttccctcc cacaacagaa taccaatcgt gcattcctta tgcgtcgagg tgatatggtt
     1981 accgcgaatg agcacggagt ccgaatccgg agcagaagtc gttatccctg ccgaacaagg
     2041 atagttgacg ggatctatgg ttaccgtatt attcgatatc tccaggcctt taacccagcc
     2101 cgggacaacg actccccaac gatcgcccgc tattgtattc tccgtaatga cgatgttttc
     2161 ggaaaggctt tgcggcaact cgttgcgcat atcgctcacc acaatcgctg caaaagtgtt
     2221 cccctcaaac gtattacggg caatcgtgac attttttaca ccacgaaact caaaattttc
     2281 cagttcgata tcgatgccac ttttgggatc gtgcttggtt tgatgagata attcacactc
     2341 agtcacgcta atattcgtcc cgctcgtgat actcaaacct tgtctaccat tatgatgcat
     2401 cttgaccttg tggatacgga catgatccgt tattcctccg ccataccctg gctccaaata
     2461 atcggcaatc atgatgccat cgtcttcgca attcgatatg tcgacgtcct cgatcaacac
     2521 atcgctcgat ccattcgcga tgttgatccc gtagcaatag ttatgcttat ccggtggatg
     2581 cgtatccttg tccccttcga tacgacctcc gatcactttt acgttcttct tatgatcaat
     2641 acttatgaac gcatatcctc cttggctacc agtgttcatc accaatttga caccggaact
     2701 catggtaagc gtaacattgt cctggagctg gatccctcca tgggtagagt attcgaactg
     2761 atgcgttgca gaggctgctt ctgctttgat cagataagtc ccatcgggga tacacacatt
     2821 gacgattccc tttttcgaag cgtcttgaat ggcttcgttg attgcttgcg tcgcatcctc
     2881 agaagggaca gtatgattcg cgtcttgata atactttttg gtcgatggat cgaagaaatg
     2941 ggcgttcgct ttcgcaccat aatccgcaac gttgatggca ttcggatcaa gacaaccatt
     3001 gggcggggtc gaactatccg gtggagacga accgtcaggt ggagtcgaac tatcggctac
     3061 gctagaacca tcagggaccg tcgcgctatc ggtcaggctt gcaccatccc ggtcctctgc
     3121 taccacggga tcagcatcgt cgctgccgca tgcagcccag ccaaaagaaa aaatacctac
     3181 catgctcccc atgacccact gccgatacat ggttacctcc ttatttcagg actaataaca
     3241 cagttccgct accttgccaa ttcaattcct ccagcctatc aaaggcagtt tgagcgaaaa
     3301 actgctccag tcctgagcct tcccctcttt atcctcgagc ctttccttcg ccttcaacgg
     3361 atgatgacgc ataagcgggc aaaagaacaa gcatgctcgc tctggaagcc gaatagccgc
     3421 acgataccgt cgcggatgtc aaacgcgccg cattgcgctg catcgagggc acgaatggcc
     3481 tcctcgacaa agaaaggttt cgtaacccgc gcctatctac acgatgcgtt catatatcgg
     3541 taattactac tactttgata agccttggcc aacgtgggat acaaggtgcc ctaccggcag
     3601 cgcaacgtcc aagacaacac gtggctcatg cttgaacgag gggctcggag gcgggatgag
     3661 ggatgggaac gcaagatcga ccacacacgt tttgtggggg cgataccgcg cttccgtttg
     3721 gttcggaccc ggattcttgc tatgatggga gccatgacac ggccatcgcc ttccgtcttg
     3781 caaaaaacgg cacagacaat gaaggttcca ccttcacaat aagggctcta tgatgcatcc
     3841 catctcgata ccattccatc gaatcccagt tgctttcctt gccctgctgc tgacagccgg
     3901 atgcggagac gagaaaacgt cgtccgacac gacacctcac gactccggga tgaaggaaaa
     3961 cgacgcgcag actggcggtc aaggcggaag cgcaggcaca gcagcggagg gaggcgcggg
     4021 aggttccgat tgcagccttc aagacaagga ggctcaaacc caaagcgcgc gcaacgcgct
     4081 tcgggaacaa gtgccatgct tctctggatc cttcgcggtc gacaagaacg gtggcataca
     4141 cttaagcttc ttcctttcca ccgactttaa cggccccaga gcgttgttgt gccatacgga
     4201 ctcgaacgta tccgtgagca aggcccatga acaagccgct tccaaagcgc tcgaaggctt
     4261 ggatctttcg tgtttgaaag gattatatgg aattgatatc gctggtggtg cttgcgagca
     4321 gagggatacg ttggaagagc tattggccgc ttgcccacca tggtgggagc acgtaaacga
     4381 agaattagga atcgttttcg atgaaaacgg ccaggcggta tctctcaaac cggcagaagg
     4441 gaaaccgtcg gaacctgaga tcgaaacctg cgttctcgac gcccttgctg gcaagacgtt
     4501 tccctgcctg gcgaatcttg aggtgtgtgc cggcaacccc attccttgaa ggtgcttgca
     4561 gcaggtattt gactcttctt ccacgaaggt tgtcacgctc gacacgtgat tttctctacg
     4621 ggttgggtgc cattggcggt tctgacgatc aacaagggca gccagggcgc acgattccac
     4681 tcatggttga ttttgccgct tgacgctcat cagaagcagc ttcagactgc cgctttctca
     4741 tggccttttt cccgacgatg cgcgacgtgc attgccatta tcagggctgg gcagcaccta
     4801 tttgcacagg ttttggagaa aagctggaga attcgctaaa cggcggggtg ttcggtggct
     4861 tggggaacgg aacctggact gcttcaaacg aaaaaagggt aactttttgt aaccgcttga
     4921 tataaaagga ttttctggaa cggatcagcc agacacgacc ggtggggccg tcggcgcacc
     4981 ctccacgggc cgcacgcgcc gtccgagcca ggcctccagg gaaatcgctc ccgcgccatg
     5041 gaccgccagc gccagaaaac caccggcaat ggcgatattc ttgagcgcgt ggatctgctg
     5101 catatgcgcc tcggcgccct cgagtccgaa cggattgtgg aaaagcaccg tggccggcac
     5161 gaggaaaagc gcaagcccga ttgcggcgag tcgggtttgg aagccaacca cgaccatcac
     5221 gccccccacg agttctgaca ccccggcaag caaagccaaa agcatgggcg cagggagccc
     5281 cgtcgaaccg atatacgctg ccgtacccga aagatccgcg agttttccaa accccgagat
     5341 gatgaaaatg accgccagca aggtgcgtgc aaccagtaca ccgtaacgtt ccatcgttgt
     5401 tatcctttca ctccttcgca gcctcttgct tgacggcctc gacttcgatt tcgatggtga
     5461 tgcgatcccc aaccatcaca ccaccagcct cgagcacctg gttccacgtc aacccaaagt
     5521 ccttgcgatt gatcgaggtt tttgcggcat aacctgcccg cacgttgccc caggggtctt
     5581 tggtctgtcc cgcggactcg gcatccaaca ccacctcacg ggtcactccg tgaatcgtca
     5641 ggttcccgat aaggcggaag ttttcttcac cttgcttttc aaccctcaca ctttggaagg
     5701 tcaactccgg aaagttctcc acatcgagaa agtccggatt cttgaggtgc gcgtcgcgat
     5761 ccgcaacgcc cgtgtcgata ctcgaagcgt cgatcacgac ctcgaccttg gcccgggcca
     5821 tgtctccgtc ctcgatgacg atgttgccgc tccagcgtga gaattgcccg cggactttgg
     5881 caatgaccat gtgtcgaacc gagaaaaaaa ttccggagtg gctgctgtcg atttcccact
     5941 tttcaagtga cattttcgta ctccttagga atgaggaagg gccgtcgata ggcccgttgt
     6001 tttcgcctcg tcttcagcaa ggagcgggcc aagttgaatt tacctgaaaa tataggtgat
     6061 tctcatcttt cttctggtgc tgttcttctc aaaatgataa attgtttctc aaaatgacaa
     6121 ggactcgccc atgaaagctg cggatttgga cctgcgagaa cttctacact tcgaacccaa
     6181 aggaggcgtg ctgacgttcg ccggacagcg cgctttgctc ttcgatgccg tcgctttggg
     6241 ccttttgcga cgggaactcg ttaaatctct cggcctttcc ggcgcccggg gcatgctcac
     6301 ccgcttcggc tacgcccacg gatggcggac tgccgagacc ttgaaaaacg cctttccctg
     6361 ggacgacgaa tccgagtgga gacgcgcggg cgggcgcttg cacgctctgc aaggcatggt
     6421 caccttcgaa cttccttcgc gagccacaaa caccggacca gaagcgttcg ccgaggcgat
     6481 ttggcatgat tcctacgaag ccgagcaaca cctgctgcac ctcggtcgcg ccgaggagcc
     6541 cgtatgctgg accctcaccg gttttgccag cggctacctt tcctattgca acggacgtga
     6601 aatctactgc atcgaagagc gctgccgtgg caaaggcgat gctgtttgca cccttgtcgg
     6661 gcgaacacgc gaggattggg gggatgcgat cacaccgcac ctgccgtttt ataaaaagga
     6721 gtgcctcgag gaggcgctcg cacaagtggc aaccgaactc aaacgtgtcg aagagcggct
     6781 tcgcgcgcgc aaaaaggttc tcggccatga tgcaccggag tcgaccggct caggggtcgt
     6841 ggtgcagagc gaagcgatgc tgcgcgtgct cgatcttgcg cgacgcatcg ccaaggtgga
     6901 ttctaccgtg ctgattacgg gcgaaagtgg cgtgggcaag gaacgcctcg cgcggttcat
     6961 ccacgacgaa tccgttcgca ccggcggccc ttttgtcgcc atcaactgcg gcgccatgcc
     7021 cgagggcttg ctcgaatcgg aactgttcgg ccatgtcaaa ggcgcgttca ccggcgccac
     7081 ccaggaccgt cctggccttt tcgaagctgc gaatggcggg acgcttttgc tcgatgagat
     7141 cggcgaggtc tcccccccca tgcaggtaaa gttgctgcgc gctttgcaag aacgcgaggt
     7201 tcgtcgggtt ggggaaaaca aaagccgtaa ggtggacgtg cgcttgctcg cggcaaccaa
     7261 tcgtgacctg ctctccgaag tgcatggcgc acgctttcgg caagatctct actaccgcct
     7321 gcgtgtcgtg gaattgcgcg tgccgccgct tcgtgagcgt cgcgacgata tcctcgcgct
     7381 ggcgcgaacc tttctcgcat cggcttccga gcgaatgggt cgcaaggtca cgagtttcac
     7441 cccgggtgcc gccaaccagc tccttcgtta cgactggcca ggcaacgtcc gggaactcga
     7501 aaatgccatg gaacgcgcgg tcgcccttgc caagacgagc cagatcgata tcgatgacct
     7561 gccggaagag gtcgggatcg ccattccgag tgcctatgcg ccgggagatg tgcgaccgct
     7621 cgcggatgtg gagcgcgact atatccttgc ggtcgttcgg gccaaccagg gcaacaaagc
     7681 cgcggctgcc gaacagctcg aaattggggt ggcaaccctg taccgcaagc tcaaacaata
     7741 cgagtccgtg ggaggtgtgt cctgaagggc aaggtcaacg catcgaaggc tgcataccgg
     7801 aaattcatgg gttttgcggt cggtgcaaag caatcgcaaa acagcaaaaa actcctataa
     7861 aatcatatcg ttagctagtt ctcatcccag aagtcgtacg tatcgatttt tgtagctgat
     7921 cgtgatccgt tttcgctccc tcaagcgtct atatatcgta gacaacgacg actctactcc
     7981 aaggatgaaa acctatgcgt aagatgtttg agcctcagtt gcccctgctc cagcgctgga
     8041 tcgataagcc tcatgccctt gaacgtaggt ttcgcgcccc ttcaattcgt tgagtggcag
     8101 tccagcgcga taat
//
LOCUS       DAOSOR010000230         8086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8880_length_8086_cov_4.552982, whole genome shotgun sequence.
ACCESSION   DAOSOR010000230 DAOSOR010000000
VERSION     DAOSOR010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8086
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8880_length_8086_cov_4.552982"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..3553)
                     /locus_tag="PK710_13150"
     CDS             complement(86..3553)
                     /locus_tag="PK710_13150"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT10717.1"
                     /translation="MQPGTRLAGRYDAIRELGRGGMGIVYACVDIVTGEKVALKLLHT
                     ADGTPLPGTAAWFWAEARALAYLNHPGIVRARDFGVLRNNAPFLAMDIAPGLSLVDLL
                     GETELSWPAIWAITDQLFAALAHAHARGVIHGDLKHSNIMVEVLPEDLKVRVLDFGLA
                     WLLRDRFDHRIDGTAREGPMVRPHAGTVGWMAPEQIRGAIPHVGPATDLYALGCVLFQ
                     LLTGNEPYEASELEEIQRLHRNAPVPEVSLAPDVPAAVAPFVQRLLAKRPWHRFAFAA
                     DARRVFAGFRPEGPMSSWSLPVLANDDDVPTLRPPPSRDTELKPTFEIPITDQSPPGL
                     LGFGPARCIGREDKKAELCESLDQLCKAERGACHMVLLTGPAGVGKSHLAEWLCEDAH
                     ERGVAIPLRARHNRIPTPFDGIVGAVMGYLGLENADRTTIERVLMNLWQVRKEDDDAL
                     VWVAAVAEWLHPTAPGSQTEVGPSGKRFVINSHQIRWAIMRYVLTWLGQERPLLLWLD
                     DLHMAGGRDFNWIEQLEKQPLPVRMLILATTPTRGPEAAVVQQPRILELVEKLGARCL
                     EIEPLTALEMRDLLLSSAALDEGVINLAFRRSRGIPLFALQLIHAWANSGALRMRNGR
                     YFAEGVAEDEVPPTTAALWEERLSTLPEEALRAAMAASALGGDVRSEVLVPLLASLEL
                     SAFETISLLEQTQLLTKVGKDRYRWPHGLLQEHLLAKLSEQHDASRVLKQAAFALASY
                     HPAAGTRRIVRHRVTNLLRANEESEAVRVMIDFVSRSWERTRDVSATISDLRLIEGLP
                     RGSFAAMYNRWLAEALRHAGQLDEAHSLASLARRSFSYLNDPFHEAQCLRLLGHIASE
                     RGSPRDGREMVVQARATMNALHEPWGRAQCDVLLGELDYLLGNHALAIRELEAAIEPL
                     EQAQDILGRGQCQVLLAFIDLARNDAYGARKHLVSARQGFDRIGYRLGTAQCDVALAH
                     ADHRLLELESARARGQNALSAFQLLGTPRGQTAALRVIAMAALDAGDLVDAEQRATEA
                     LALFEQLGDPWGLAESKLLLAQVALARGAPEAREILQSIEIHRVQEPEPRQHWHLTMA
                     WWLAREGAFDEALHHLEQAKLTFGDERVGDHALPLSSRVASVPWPDAYAQRLDAVLLK
                     GSVSPKQEP"
     gene            3780..5195
                     /locus_tag="PK710_13155"
     CDS             3780..5195
                     /locus_tag="PK710_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT10718.1"
                     /translation="MLFRRKLATALLTAGVVLTPLAEVARANDEPAPSHERTPRSQTP
                     SARRSGYSLQQCVAFAERNYPTIAQARAKANYYRAQLDEARSAPFSQWKASAGIALAP
                     TSRGTSVYSPDTDVALKSNMGLAWRTNVEGVIPLFTFGKISNLIDAAENQVELGEHQI
                     AKERNQVRLDVRKAYYGLLLARASSTLLTEAISKLDKAIDKLAQQVEDGDADEFDLFK
                     LQTYRAEIDARMAEALRYESVALVSLAFLTGIQTKFDINTDQLEPSPHVLGPVARYLQ
                     AARVHRPEINMARAGVRAREAQASLARAKMYPDLALGMSAGWTRAPEIDDQLNPYVKD
                     NANFVSLGFGLVMQWNLDLLPAAARLEQARAQLEETRAIERMALGGVGVEVETAYAEV
                     ASAQKREQAYGRAESLAKKWLIGVQQGIDLGTEEEKALIDPAKEYAQQRFNHLNAIMD
                     LNVAMSKLALVTGWDAIAPQG"
     gene            5210..5803
                     /locus_tag="PK710_13160"
     CDS             5210..5803
                     /locus_tag="PK710_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10719.1"
                     /translation="MIHPLQRLTPLLFMGLLFACEDKTKTSSPTAASTSAPAHPSGLS
                     PDPLPPLKLESFQGLGLSFTYPKPYQVETVTTNPQIHQIAIDHNQEPGVLVIRFDPSH
                     PAKPMVLDEIGEATRQRMDAQATMTPSTLMVAGQRVEARTIHSTQLGLVSSTDVVAIV
                     TIKGTNYVVLTHTADADAQRAQRMFDTVLQSLAGQSP"
     gene            complement(5851..>8086)
                     /locus_tag="PK710_13165"
     CDS             complement(5851..>8086)
                     /locus_tag="PK710_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOT10720.1"
                     /translation="GVAGTAGDAGPEDVSVEDVSSDVVDDDPSSDISPDIADTGPPSC
                     PVTFKYKPGGPATKIVVAGEWQGFDLATAPVMSPDGTGSYQVTVNLEPGLYAYKVVVD
                     GDWRLDASQPRRKYVGTPLVENSAIKVADCRMPSWRVETSEVTRPSAGEGTYQAVLRF
                     VDGIEGTGPDIKGYSFALQHNGTDKPVSLTPDNSGDVVVSLTGLQDGKYRLLATPRAG
                     NGREGTPLRLVFWVEREPFSWKDALIYMVLTDRYRDGDASNNPGATQGADSRGDWQGG
                     DLQGVRDSIVDGTLDALGVRAIWLTPFQTNPAGAFSASDGVHKVTGYHGYWPVKARQV
                     DPRIGGETALRELVREAHAHGIRILQDFVLNHVHQEHEYVASHPTWFRTGCVCGTNNC
                     DWTAHALDCMFASYMPDIDHRVPEANAAFVSDAVWWLDEFDLDGLRVDAVKHVEESAT
                     RNLAAEVRETFEAAGTRYFLMGETAMGWNECADPCNDENYGTISRYIGPFGLDGQFDF
                     VLYHGVSYRTFAEDSKGMLHADYWFQHGMSKYPADAIMTPYIGSHDTPRFSTLADPDN
                     ASKAGNQWENIATSPSQVLPYQRTRIGFAWLLNLPGAPLLYYGDEYGQWGGADPNNRL
                     MWKKPSSLTSDEQDTLAFVQKLGMARKNVPALRRGDYVSLFVTENTLIFGRKTSSGSA
                     IVALTRAGSAQSVTVPDVTTKVGIASGTVLHDHLGGPNVTVSNGSISLSIPAKGAVVL
                     SQSP"
BASE COUNT         1853 a         2517 c         2124 g         1592 t
ORIGIN      
        1 aaacgctacc cctgtcgggt accaccgaca cataactatt gaattttaca acatattact
       61 ggaatcccac gacgaaatcc ggttctcagg gttcttgttt cggtgagacg cttcctttca
      121 gcaacaccgc atcgagacgc tgtgcataag catctggcca cggcacggag gcgaccctcg
      181 aagacaacgg aagggcatga tcgcccactc gttcatcgcc aaaggtcagc ttggcttgtt
      241 cgagatgatg taaggcctcg tcgaacgcgc cttcacgggc gagccaccaa gccatcgtca
      301 agtgccaatg ctgtcggggt tcaggttctt gcacgcgatg gatttcgatg gactgcagga
      361 tttcacgagc ctctggcgct ccccgagcga gcgcgacctg tgccagcaga agtttgcttt
      421 ccgccaatcc ccaggggtct ccgagttgtt cgaaaagcgc aagggcttcc gtggcccttt
      481 gctccgcgtc caccaggtct ccggcatcga gagccgccat ggcaatgacg cgcaaggcag
      541 cggtttgccc gcgaggggtt cccaacaatt gaaacgccga caatgcgttt tgtcctcgtg
      601 ctctcgcgga ctcgagttcc aatagccgat gatccgcatg ggcaagagcc acatcacact
      661 gcgcggtacc aaggcgatac ccaatgcggt cgaatccctg ccgagcggat acaaggtgtt
      721 ttctcgcacc gtaagcatca tttctggcga gatcgatgaa cgcgagcaac acctgacact
      781 ggcctcgacc aaggatatct tgtgcctgtt ccaaaggttc gatggccgct tcgagttcgc
      841 gaatggccaa cgcatgattt ccgagcaggt agtcgagttc tcccaacaaa acatcgcatt
      901 gagcgcgtcc ccaaggctcg tgaagcgcat tcatcgtggc gcgagcctgc accaccatct
      961 cccgtccatc tcgtggagaa cctctctccg atgcgatatg gccgagcaaa cgcaggcatt
     1021 gcgcttcatg aaaaggatca ttgagatagg agaaggatcg tcgtgccaac gaagcgaggg
     1081 agtgggcctc atcgagctgc ccggcatgac ggagcgcttc ggcgagccat cgattgtaca
     1141 tggcggcgaa gcttccgcgc ggcagccctt cgatgaggcg aaggtcgctg atcgtagcgc
     1201 tcacgtctcg cgtgcgttcc caagagcgag acacgaaatc aatcatgacg cggacggctt
     1261 cgctttcttc gttggcgcgc agcaagttgg tcacgcgatg acgaacgata cgacgtgtgc
     1321 cggcagcggg gtgataagag gccaacgcga aggcagcctg tttcagaacg cgggaggcgt
     1381 cgtgctgttc ggatagtttc gcgagtaagt gttcttgcaa aaggccgtgc ggccagcgat
     1441 agcggtcctt accgactttc gtgagaagtt gggtctgttc gagcagcgag atcgtttcga
     1501 aagccgacaa ttcgagcgat gcgagcaagg ggaccagaac ctcgctgcgc acatcgcctc
     1561 ccagcgccga tgccgccatg gcggctcgca atgcttcttc gggcagtgtg gagagacgtt
     1621 cttcccacaa cgcggcggtg gtaggcggca cttcatcttc cgctaccccc tccgcgaaat
     1681 agcgaccatt gcgcatgcgc agggcgccac tattcgccca ggcatgaatg agctgcaaag
     1741 cgaaaagggg aataccgcgg gaccgacgga acgccaagtt gatcacacct tcgtcgagcg
     1801 ccgcggaaga aagcagcaag tcacgcattt cgagcgcggt caggggctcg atttcgaggc
     1861 aacgagcgcc gagtttttcc acgagttcga ggattcgcgg ctgttgcacc acggctgcct
     1921 caggacctcg tgtgggcgtc gtagccaaaa tcagcattcg cacgggaagc ggctgctttt
     1981 cgagttgctc gatccaattg aagtcacgcc cacccgccat gtgcaaatca tcgagccaca
     2041 acagcaaagg ccgctcctgc ccaagccagg tgagcacgta gcgcatgatc gcccatctaa
     2101 tctgatgaga gttgatgacg aatcgtttgc ccgaaggtcc gacttccgtc tgcgagccgg
     2161 gggccgtcgg atgcaaccat tccgcaacgg ccgccaccca taccaacgcg tcgtcatctt
     2221 ccttgcgaac ttgccaaagg ttcatcaata cgcgctcgat cgtggttcgg tcggcgtttt
     2281 cgaggccgag ataccccatc accgcaccca cgataccgtc aaatggcgtt ggaatcctgt
     2341 tgtgtcgagc acgcaagggg atggcaaccc ctcgctcgtg agcatcttca cacaaccact
     2401 ccgcaagatg actttttcca acacccgcgg gccccgtgag cagcaccatg tgacacgcac
     2461 cgcgctcggc cttgcaaagc tggtctagcg attcacacag ttctgctttt ttatcctctc
     2521 gaccgatgca acgagcaggc ccaaacccta gcaacccagg gggcgactga tccgtgatgg
     2581 gaatctcgaa cgtgggcttg agttccgtat cccgcgacgg tggcggccgg agcgtaggaa
     2641 cgtcatcatc gttggcgaga acgggcaagg accaagaact catcggtcct tctggacgga
     2701 aaccagcaaa tacccgtcga gcatcggccg caaatgcgaa tcgatgccac ggtcgtttgg
     2761 caagcaagcg ctggacgaaa ggagcgaccg ccgcggggac gtcaggtgcc aaagacactt
     2821 cgggcaccgg agcgttacga tgcagcctct ggatttcttc caactccgac gcttcgtagg
     2881 gctcattgcc ggtgagaagc tgaaacaaga cacatcccaa ggcatagaga tccgtggcag
     2941 ggcctacgtg cggaatggca ccccgaattt gctccggagc catccacccc acggtccccg
     3001 catgcggacg aaccatcgga ccttctcggg cagtgccgtc gatacgatga tcgaagcggt
     3061 cacgtaataa ccaggccaaa ccgaagtcca gcacacgcac cttcaaatcc tcaggcaaca
     3121 cctcgaccat gatgttgctg tgtttcagat caccatggat gactcctcgc gcgtgtgcat
     3181 gggcgagagc cgcaaaaagc tggtccgtaa tggcccaaat cgcaggccaa ctcaattccg
     3241 tctcccccaa taagtcgacc aaagacaaac ctggcgcgat atccatcgca agaaatgggg
     3301 cgttgttgcg cagcacccca aagtcacggg cccgaacgat tccaggatgg ttcagatacg
     3361 caagcgcacg cgcctcggcc caaaaccatg cagccgttcc cggcagcggt gtaccatccg
     3421 ccgtatgcag tagcttgagc gctactttct cgcccgtgac gatatcgacg caggcgtaca
     3481 caatgcccat gccgccgcgc ccgagttcac ggatcgcgtc gtagcggccc gcgagacgag
     3541 tccctggttg caagacgttg tccacatggg caagtgtaat cgaccctcgt tcatcacggc
     3601 aatccgaagg tccgttcttc cgcccattgg gttaccttga ttcaccacgg caaggggaag
     3661 gacgaacagc aagcaacgta aaacagccgt gcttcctaca ggacaccatc gttcttgggg
     3721 taaccaaaac gcaagggctt ctccccgcac cgtgatctgc tagggtacgc gagccatcca
     3781 tgcttttccg acgcaaattg gccacggcgt tgttgactgc gggagtcgtt ttgacaccgc
     3841 tcgcggaagt ggctcgcgcg aacgacgagc ctgcgccaag ccatgagagg acgccacgga
     3901 gccagacacc ttctgcgcgg cgttccgggt attcgctgca acaatgtgtt gctttcgcag
     3961 aacgtaacta cccgacgatc gcccaggcac gagccaaagc caattactac cgcgcccaac
     4021 tcgacgaagc tcggtcggca cctttcagcc agtggaaagc aagcgctgga atcgccctgg
     4081 ctcccacctc cagaggtacg tcggtgtata gcccggatac ggatgtcgcc ctcaaatcca
     4141 atatgggcct tgcctggcga accaatgtcg aaggggttat ccccttgttt acattcggga
     4201 aaatatccaa cctcatcgat gcggccgaaa accaagtcga gttgggcgag caccagattg
     4261 ccaaagaacg caaccaagtg cgcctcgacg ttcgcaaggc gtattacggg ctgttgctcg
     4321 cacgcgcttc ctccaccttg ttgaccgagg cgatcagcaa gttggacaag gccatcgata
     4381 agcttgcgca acaggtggaa gacggtgacg ccgacgagtt cgacttattc aaactgcaaa
     4441 cctaccgcgc ggaaatcgac gctcgcatgg ccgaagcctt acgatacgaa tcggtcgctc
     4501 tcgtgagtct tgcgttcctc acgggcattc aaaccaaatt cgatatcaat accgatcaac
     4561 tcgaaccttc ccctcacgtg cttggtcccg ttgcccgata tcttcaagcc gcccgtgttc
     4621 atcgtccgga aatcaatatg gctcgcgccg gggttcgcgc cagagaagca caagcttccc
     4681 tcgctcgcgc caaaatgtat ccagatctcg ccctgggaat gagcgcgggc tggacacgcg
     4741 ccccagaaat cgatgaccaa ctcaacccgt atgtcaaaga caatgccaac ttcgtttccc
     4801 tcggttttgg acttgtcatg cagtggaact tggatctcct tccggcggct gcccgcctcg
     4861 agcaagcccg cgcgcaactc gaagaaaccc gagccatcga acgaatggcc ttgggtggcg
     4921 tgggcgttga agtcgaaacc gcatacgccg aagtcgctag cgcgcagaag cgggaacaag
     4981 cctacggtcg agccgaaagc ctcgcgaaaa agtggctcat cggtgtgcaa caaggcatcg
     5041 atctaggcac ggaagaagaa aaagcactca tcgatcccgc caaagaatac gctcaacaac
     5101 gcttcaatca cctcaatgcc atcatggacc tgaatgtagc gatgtccaaa ctcgcccttg
     5161 tcaccggatg ggacgccatc gccccgcaag gatgaccccc cattagacca tgattcatcc
     5221 gctccaacgc ctcacaccat tgcttttcat gggccttctc ttcgcctgcg aggacaaaac
     5281 caaaacctct tcgcccaccg cggcctcgac ctccgcaccg gcccatccct cgggactgtc
     5341 ccccgacccc ttgcctcccc taaaactcga atcatttcaa ggtcttggcc tttccttcac
     5401 gtatcccaaa ccctaccaag tcgaaacggt aaccaccaac ccccaaattc accaaatagc
     5461 catcgatcac aaccaagaac ccggtgtgct cgtcatccga ttcgatccct cccatcccgc
     5521 caaacccatg gtcctagacg aaatcggcga agctacccgt cagcgtatgg acgcacaagc
     5581 taccatgacg ccttccactc tcatggtagc tggccaacgc gtcgaagcac gaaccatcca
     5641 ttccacccaa ctgggattgg tgtcttccac ggacgtggtg gccatcgtta ccatcaaagg
     5701 aacgaattat gtggtgctca cgcataccgc cgatgcggat gcgcagcgag cccagcgcat
     5761 gttcgacacg gttctacagt cgctcgcggg ccagtctccc tgacgttcac ggcgtgagat
     5821 acgtgcgact atcgaaggac gttctttccc tcatggcgat tggctcaata cgaccgcgcc
     5881 tttcgcggga atcgacaacg atatcgagcc gttggacacc gtaacgttcg gacctcccag
     5941 atgatcgtga agaaccgtac cggaagcgat ccctaccttg gtggtcacat cgggcacggt
     6001 caccgattgt gcgctcccgg cacgcgtaag cgccacgatg gcggaaccgc tcgatgtttt
     6061 tcttccaaat atgagcgtgt tttcggtgac gaacagagaa acgtaatccc cacgacgcaa
     6121 agcggggaca ttctttctgg ccataccaag tttttgaacg aacgcgaggg tgtcctgctc
     6181 atcggatgtg agcgaagaag gctttttcca catgagccgg ttgttgggat cagcgccacc
     6241 ccactgaccg tattcgtcgc cgtaatacaa tagcggtgcg ccaggaagat tgagcaacca
     6301 cgcgaatcca atgcgcgtgc gctggtacgg caacacctgg gagggcgaag ttgcgatatt
     6361 ttcccattga ttgcccgctt tcgatgcatt gtcaggatcc gcaagcgtcg aaaaacgagg
     6421 cgtatcatga ctgccgatat acggcgtcat gatcgcatcc gccggatatt tgctcatgcc
     6481 gtgctggaac cagtagtccg catgaagcat ccctttgctg tcttctgcaa acgttcgata
     6541 agaaacaccg tggtacaaca cgaaatcaaa ctgaccatcg agaccgaaag gaccaatgta
     6601 acgtgaaatc gtcccgtagt tctcatcgtt gcaagggtcc gcgcattcgt tccaccccat
     6661 ggcggtttcg cccatgagaa aatagcgcgt tccagccgct tcgaaggtct cgcgcacctc
     6721 cgccgccagg ttgcgcgtgg cggattcctc cacatgcttg accgcatcga ctcgcaatcc
     6781 atcaaggtcg aattcatcca accaccacac cgcatcggaa acgaacgccg cgttcgcctc
     6841 cggaacgcga tgatcgatgt ccggcatgta gctggcgaac atgcaatcga gggcatgagc
     6901 ggtccaatca caattgttgg ttccacatac acatccggtg cggaaccacg ttgggtgcga
     6961 agccacatat tcatgctctt gatgaacgtg attcaacacg aagtcctgaa ggatgcgaat
     7021 tccatgggca tgggcttcgc gaaccagctc acgcaaagct gtctctccac cgatgcgagg
     7081 atcgacttgc cgtgctttta caggccaata gccgtgataa cccgtgacct tatgcacacc
     7141 atcggaggcc gaaaaagccc ccgctggatt ggtctgaaac ggtgtgagcc aaatggcccg
     7201 aaccccaagc gcatcgagcg tcccatccac aatggagtct cgaacgcctt gtaagtctcc
     7261 cccttgccaa tccccccgtg aatccgcccc ttgcgtcgct cccggattgt tcgatgcatc
     7321 cccatctcgg taacgatccg tcaaaaccat ataaatcaag gcgtctttcc aggaaaacgg
     7381 ctccctttcc acccaaaaca ccaatcgtaa cggcgttcct tccctgccat tgcccgctcg
     7441 tggcgtcgcc aacaaccgat acttgccatc ttgcagccca gtcaaagaca ccacgacatc
     7501 accgctattg tccggcgtca gagaaaccgg tttgtcggtt ccgttgtgtt gtaacgcaaa
     7561 cgaataaccc ttgatatctg ggcccgtccc ctcgattcca tccacgaagc gaaggaccgc
     7621 ttgataggtt ccctctcccg ccgaaggacg agtcacttca ctggtttcga ctctccacga
     7681 tggcatccta caatccgcaa ccttgatcgc cgagttttcc acaagcggtg tgccaacata
     7741 ttttctgcgt ggttgagagg catcgagccg ccaatcgccg tccaccacca ccttgtacgc
     7801 gtaaagccca ggctcgagat tgacggtcac ctggtaggaa cccgttccat caggactcat
     7861 gacgggcgca gtagccaaat caaatccctg ccactcgcct gccaccacga tcttggtggc
     7921 aggtccacct ggtttgtact tgaacgtcac cggacaagac ggaggacccg tgtcggcaat
     7981 atccggcgaa atatccgaag aaggatcgtc gtcgaccacg tcggaggata cgtcttcgac
     8041 agacacatcc tcaggtcccg catctccagc ggtgcctgcc acccca
//
LOCUS       DAOSOR010000231         8085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8883_length_8085_cov_3.842092, whole genome shotgun sequence.
ACCESSION   DAOSOR010000231 DAOSOR010000000
VERSION     DAOSOR010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8085
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8883_length_8085_cov_3.842092"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="PK710_13170"
     CDS             complement(<1..823)
                     /locus_tag="PK710_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10721.1"
                     /translation="MATKSLYSVPVGCGALVAFAMLFGVSPGYADEPYKATERPVLNE
                     PGEVTAVVDAFDDDDLFDLHLTLGYQHMWKNANIVRETSIDQGSLSSGGYVADTMNVA
                     KYSGSTSRLNTRVDVGLYKDVALYLRMPIILNDSSKLSGLDGSDRVQSVVLAGMPGEQ
                     LFRLPFESPDRSGIEYLALGFDVNIMNQARDATKPTWLFGFEGRFSLSEPMHACGAGG
                     GNQGNKQVSCADPSDVNRDGTAGNYAYTDHTGQTYQLYGTKGRERSAGVSRGTTGI"
     gene            complement(1008..2381)
                     /locus_tag="PK710_13175"
     CDS             complement(1008..2381)
                     /locus_tag="PK710_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOT10722.1"
                     /translation="MGTNILFHAETDVGRKRDHNEDNFLVDKGLSLFMVADGMGGHAA
                     GEIASALAVRTIHEKIKGERTLLDDFLSGASGASRVTVKGVLNLLEYSVQQACKHIYE
                     AAQVDVAKRGMGTTLSLLLVCGNRGFIAHVGDSRIYLVRNGKGRQITEDHTVFNELVK
                     RGKLSRDQIEKVAQKHAITRALGVYEQVEVDTLSFELLPGDQLVLCSDGLHGYLDSMQ
                     ELEAPLAMPEGQAARALIAMANEKGGKDNITVVLVRVVGEGTAMEAQRAQRLALKREV
                     LMRMPLFGKLSERELMRVMQVAEVRTYEPGQQVIRDGDRGDELFIMLSGQVKVFKGDA
                     ELSTLGQGEHFGEMALIRSQPRSADVMAETKSEIIAINRTDFFDILRKEHEMAVKLLW
                     QFLGVLADRLDRTTKDLRTAREEMEIEDITADIFRDEQSEDTVSAEEQDIHSRPTIPN
                     TINRDRE"
     gene            complement(2476..3363)
                     /locus_tag="PK710_13180"
     CDS             complement(2476..3363)
                     /locus_tag="PK710_13180"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HOT10723.1"
                     /translation="MKNPADLLRSIPMFEGLDDQDVEDLAAELVERRYSAGQVVFEAG
                     EAGDTMYLVAEGTVNIHLPGDPTTRVSLADLTVGKVFGEVALFDDKPRSASAQAMTDT
                     VLLELGRTVLTSYLERRPRAAMAILRTMSERLRQTNAMLSERAARNVIEEFEKGMGWA
                     DRLADRVAELNGSWSFILILSGLTVGWVLINIPGVVFSHAPDEYPYQFFNLLLAVLVA
                     LQGPLIVMSQNRQAAKDRKEAETDFKVNLKNEVNIETLLKELREFRALAAGRRPGAVP
                     TGNVKPERSGLSANPRMDA"
     gene            3465..4394
                     /locus_tag="PK710_13185"
     CDS             3465..4394
                     /locus_tag="PK710_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT10724.1"
                     /translation="MNEAIAAQCDAAFLILDHAVARAEGAPTTAPNLRVTPDFPLATR
                     TAPQQVRLRLDRTLMAPDPESGLDALLDYGVLDALLPEVSALVGFGDGEWRHKDVWKH
                     TKQVVRQAEARLSVRWAALLHDVGKVKTRSISADGQVHFFGHAELGARMFDRIGKRLG
                     FFEPPLHRSIRFLILHHLRANQYEKDWTDSAVRRFAREIGAELDDLLCLSRADITTKR
                     PEKKRRGLQQIAELSERIRELAVLDARVPPLPSGVGNEIMDAFGIPPSRLIGQIKQAL
                     EQAIEEGELPGHEPSAFYVAFLKENRTRFGLPE"
     gene            complement(4404..4979)
                     /locus_tag="PK710_13190"
     CDS             complement(4404..4979)
                     /locus_tag="PK710_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10725.1"
                     /translation="MGKMIIPMVLGGLLILGLGCSDPVFVSFPDGHQPEMTDEPSSEA
                     ATASEPQPSQDSVEAEAPSSPWPFQDAGRGEEEAQERQEGGVVEGEADAEDAMPSTME
                     PLESKVFFIQPSEGAALSNPLFFEIGARHVDYVQIFSDSIPLTELWEPVLTKSGRLWI
                     SEPGFHSLRLVGYDQQHRERAWDEITITVTE"
     gene            complement(5130..5732)
                     /locus_tag="PK710_13195"
     CDS             complement(5130..5732)
                     /locus_tag="PK710_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10726.1"
                     /translation="MRTSDCVAGGWLISAGLAAMACEGGLPEPGVQAPNDPVQVSLPA
                     PKMEKLGDYEFEALATLKLEARVLGKERYWFGRESELAPYDLALGWGAMSDSAIYGKL
                     EIEQYGRWYHYRWDAKGPPIPVDEIIRHSGNMHVIPANGDVKSALAAVCRHDIVRLEG
                     YLVEIRGSDGWRWRSSLSRQDSGAGSCEVMVVKKVELRPP"
     gene            complement(5762..6262)
                     /locus_tag="PK710_13200"
     CDS             complement(5762..6262)
                     /locus_tag="PK710_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10727.1"
                     /translation="MKHQPENVRYFRVELVLRYTRISCMPYRATFPAAKVVQAQHADD
                     AVRQACLCLDRDLEDILAQVNALNVSEVWVDILSVQLLKQAQDPVWNPWWFPTDQAIP
                     SYIIVRSVGEAGLSKESPTPEKSPESSDPLPCYGRPIDKEYRRTTAVVTTSAENWEAS
                     GPAMGE"
     gene            complement(6259..6822)
                     /locus_tag="PK710_13205"
     CDS             complement(6259..6822)
                     /locus_tag="PK710_13205"
                     /inference="COORDINATES: protein motif:HMM:NF016102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HOT10728.1"
                     /translation="MRYFAFLLLAFVLEVFVLSAVGSWIGVWKTLGLMLVTGVLGGVL
                     VKRGGIRVWSLVRQSLSEGQLPSDGILSGFLILAAGVFLLLPGFLTDLFGLLLLVPRF
                     RCMVAERLRAVMEKKARQGVMQVVALDPQGWEQAPEQHGWMPGAAEFFSKSGHGEVVD
                     TEGVEIQEGLLLGPGSSDDSIREDEEP"
     gene            complement(7007..>8085)
                     /locus_tag="PK710_13210"
     CDS             complement(7007..>8085)
                     /locus_tag="PK710_13210"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT10729.1"
                     /translation="AVDRSTGLLRGKTGAVRLAISAGVPIVPVGVSGTGRALPPEIYP
                     RLEVLRMPGASPIRIRFGEPIDYSQYHGQEVDKEVLRELTDELMRRISELVDHRSNYA
                     PIEVPIPAPPAYEKVGVLLLHGFTSSTDTVDGLLPFLEKQGIDYERPILRGHGTRYQD
                     MRGVTARDWYVDADRALLQLWNRVDRVVVVGLSMGGLVGLELAMRHPDKIAGLVTVAA
                     ALKFKDPLAMFTSLLSKGVRYWPSPESFNDPTLAKRCTNYPMFATDSFASLYDYSRRI
                     AERLPEVHVPIRILQSKKDQIIAPESANIIYEKVSSPIREMVWFEHSGHEMMQDMEAQ
                     DVFREIMAFVSYFQRDPTAVSSPD"
BASE COUNT         1797 a         2647 c         1960 g         1681 t
ORIGIN      
        1 cgataccggt ggtgccacga ctcacccccg cggaacgctc cctccctttc gttccataga
       61 gttgatacgt ctgcccagta tgatccgtat acgcgtaatt acccgccgtc ccatcgcgat
      121 tgacgtcgga tggatctgca caggaaacct gcttgttgcc ttgattgccc ccccccgcac
      181 cacaggcatg catcggctca ctgagactga accttccctc gaaaccaaac aaccacgtgg
      241 gtttcgtggc atcccgtgcc tggttcatga tgttcacatc aaaccctagc gcgagatact
      301 cgatacccga tcggtccgga gattcgaagg gcagacgaaa caactgctct cccggcatcc
      361 ccgcaagcac gacgctctgc acccgatcgc ttccatcaag acccgacaac ttgctcgagt
      421 cattcaagat gatcggcatg cgaaggtaaa gcgcgacatc cttgtacaac cccacatcca
      481 cccgcgtgtt gagcctcgaa gtcgagccgg aatacttcgc cacgttcatc gtgtccgcca
      541 cgtatccgcc cgacgacaat gagccttggt caatcgaggt ttcacgcacg atgttggcgt
      601 tcttccacat atgttgatag ccaagggtga ggtgcaaatc gaaaaggtcg tcgtcgtcga
      661 aagcgtcgac tacggccgtc acctcaccag gctcgttgag tacgggccgt tccgttgcct
      721 tatacggctc gtccgcataa ccgggcgaaa caccaaaaag catcgcgaac gcaacgaggg
      781 ccccacatcc caccggcacc gaatagagcg atttcgtggc cattaagcac atgtccgtgt
      841 cgcggacggt aatggcctgg gtgatgggat gtcaatgaat tactccccga cgggccgatc
      901 cttgggtttc cccgaacgtc cgacgctgga tttcatggct ttggattcga ggcttgccga
      961 agggaaaaag cgcggtcgaa cctttggggc aagggagcga gtccctttca ttccctatct
     1021 cggttgatcg tattcggaat ggtgggacga gaatgaatgt cctgctcctc cgccgacacg
     1081 gtgtcttcgg actgttcatc gcgaaaaata tccgcggtga tgtcctcgat ttccatttcc
     1141 tcccgcgccg ttcgcaagtc tttcgtggtc cgatccaacc ggtcggccag aacaccgaga
     1201 aattgccaga gcaatttgac ggccatctcg tgctcttttc gtaaaatatc aaagaaatcc
     1261 gtacggttaa tcgcaatgat ctcgcttttg gtttcggcca tgacatccgc gcttcggggc
     1321 tggcttcgaa tcaacgccat ctccccaaaa tgctccccct gtcccaaggt cgacaattct
     1381 gcatcgccct tgaacacttt gacctgaccc gacagcatga tgaaaagctc atcacctcga
     1441 tccccgtccc gaatcacttg ttgtcctggc tcgtaggttc gcacctccgc cacttgcatc
     1501 actcgcatca attcacgctc ggatagcttg ccgaacaacg gcatgcgcat caacacctcg
     1561 cgcttgagcg ccaaacgctg agcacgctgc gcttccatcg ccgtgccctc gcccaccacc
     1621 cgcaccaaca ccaccgtgat attgtctttt cccccttttt cattggccat cgcgatgagc
     1681 gctcgagccg cctgcccctc cggcatcgca agaggtgcct cgagttcttg catgctatcg
     1741 aggtagccat gcagcccatc cgaacacaat acgagctggt cgcccggcag caattcgaac
     1801 gatagcgtgt ccacctctac ctgttcgtaa acccccaacg ctcgcgtgat cgcatgtttc
     1861 tgcgcaacct tctcgatttg atcccgcgac aacttacccc gtttgaccag ttcgttgaaa
     1921 accgtatggt cttccgtgat ctgacgcccc ttgccgtttc gtacgagata gatccgcgaa
     1981 tcgcccacgt gagcaatgaa accccggttc ccacacacca aaagaagcga caacgtcgtg
     2041 cccattcccc gtttggccac atccacttgg gccgcttcat agatgtgctt gcacgcctgc
     2101 tgaaccgaat actccagcag gttcaacacc cccttgaccg tcacccgcga agcccctgaa
     2161 gctcccgaca gaaagtcgtc gagcaacgtt cgctcgcctt ttatcttctc gtgaatcgtc
     2221 cgaaccgcca gcgcactcgc tatctcaccc gccgcatgcc cccccatccc gtctgccacc
     2281 atgaacaacg ataatccctt gtcaacaagg aagttgtctt cattgtggtc gcgtttgcgt
     2341 ccaacgtccg tctcggcgtg aaacaggatg ttcgtcccca tgcctcgttc cgtcgtgtga
     2401 ttgataaccg accatactca atcgccatgg tcgatgcgag ccctggcgca cgttggccca
     2461 acccatccgt gtcgctcaag catccatccg cggattcgcc gacagtccag accgctccgg
     2521 tttgacgttt ccagtcggta cggcccctgg tcgacgcccc gccgccaacg ctcggaactc
     2581 tcgaagttcc ttgagcaatg tctcaatatt gacctcgttc ttcaaattca ctttgaagtc
     2641 cgtctccgct tcctttcgat ccttggctgc ctgtcggttc tggctcatca cgatgagcgg
     2701 cccttgcaac gccaccaata ccgcaagcaa taaattgaaa aattgatagg gatattcgtc
     2761 gggcgcgtgc gaaaacacca ctccaggaat gttgatgagc acccatccta ccgtcaaccc
     2821 cgataggata aggatgaacg accaactgcc attcaactcc gccacccgat cggccagacg
     2881 atccgcccag cccatcccct tttcaaactc ctcgatcaca ttgcgtgccg ctcgctcgct
     2941 caacatcgcg ttggtctgac gcaacctttc ggacattgtt cgtaaaatgg ccatcgccgc
     3001 ccgcggacga cgttccaagt acgatgtcaa cacggttctt cccaactcca aaagcaccgt
     3061 atcggtcatg gcttgggcac ttgcactccg cggcttgtcg tcgaatagcg cgacttcccc
     3121 aaatactttt cccacggtca agtccgccaa cgacacgcgc gtcgttggat cgcccggcag
     3181 atgaatattc accgtgccct cggcgacaag gtacatcgta tcgcccgcct cccctgcttc
     3241 gaatacgacc tggcccgccg aatagcgtcg ttccacgagt tccgcggcga ggtcctcgac
     3301 gtcctgatca tcgagcccct cgaacatcgg aatgcttcgg agcaggtcag cggggttttt
     3361 cacgacccgc tcagtgtggc gcgcctttcc ctcacttgca atacttgcga tggcggaagg
     3421 cgactctccc tttcgttcct tgcccaatgt gctagacgac gcgcgtgaac gaagccattg
     3481 cagcacaatg cgacgctgcc tttctcattc tcgaccatgc cgtcgcacgc gccgaagggg
     3541 cgcccaccac cgcccccaac ctccgcgtca caccagattt tccactcgct acacgcaccg
     3601 ctcctcaaca agtgcgcctc agactcgacc gcacgctcat ggcccccgac ccggaaagcg
     3661 gcctcgatgc tctgctcgat tacggtgtgc tcgacgccct gcttccggaa gtctctgcct
     3721 tggtcggatt cggggatggc gaatggcgtc ataaagacgt ttggaaacat acgaaacaag
     3781 tcgttcgaca agcagaagct cgcctgtccg tgcgctgggc tgcccttttg cacgatgtgg
     3841 ggaaagtcaa aacacgaagc atcagcgccg acggccaagt tcatttcttt gggcatgcgg
     3901 agttgggcgc acgcatgttc gaccgcattg gaaaacgatt gggatttttt gagccccccc
     3961 tgcaccgaag catccgcttt ctcatcttgc accaccttcg cgccaaccaa tacgaaaagg
     4021 attggaccga tagcgcggtg cgcagattcg cacgggaaat cggcgccgaa ctcgatgacc
     4081 tgttgtgcct ctcgcgcgct gatatcacca ccaaacgccc ggagaaaaaa cgccgcgggc
     4141 tgcagcagat cgcagaactt tctgaacgaa ttcgcgaact tgccgttctc gacgcccgcg
     4201 tgcctcccct gccctccggc gtcggcaacg aaatcatgga cgccttcggc atcccccctt
     4261 cacgcctcat cggccaaatc aaacaagccc tggaacaagc catcgaagaa ggcgaactgc
     4321 cgggtcacga accctccgcc ttctacgtcg ccttcctcaa agaaaaccga acccgattcg
     4381 gcctgcccga atgacccgac gcatcattcc gtcacggtta tcgtgatctc atcccaagcg
     4441 cgttcccgat gctgctggtc gtagcccacg agccgaagcg aatgaaaacc aggctctgaa
     4501 atccacaaac gccccgactt cgtcaacaca ggctcccaca actccgtcaa aggaatcgaa
     4561 tccgaaaaaa tttgtacgta atccacgtga cgagcgccaa tctcgaaaaa gaggggattc
     4621 gatagcgccg ccccctccga cggttggatg aaaaacactt tgctttccaa gggttccatc
     4681 gtggagggca tggcatcctc cgcgtcggct tccccctcga caacaccccc ctcctgcctt
     4741 tcttgggctt cctcctcgcc tcgccccgca tcctggaacg gccatgggga tgacggcgct
     4801 tccgcttcga ccgagtcttg agaaggctgc ggttcagaag ccgtggccgc ctcagaggaa
     4861 ggttcatcag tcatttcagg ttgatgaccg tccggaaatg aaacgaaaac cggatccgaa
     4921 catcccaaac ccaagatcaa caacccgcct agcaccatgg ggataatcat cttgcccatg
     4981 cccaaggaac tacagcacat atacctatat tgccagatat aaaaatacga ttagctacat
     5041 tgtagcaata ctgcgtttgt ccgctatgtt caaacacccc cgtggcaaag gacaccattt
     5101 cctcgcctcc ccgcctgaaa aaggcattgt catggggggc gaagttccac ctttttgacc
     5161 accatcacct cgcaggaacc agcccccgag tcctgccgtg atagcgacga gcgccatctc
     5221 cacccgtcgc tcccccgaat ttccacaaga tagccttcga gacgcacgat atcgtgtctg
     5281 cacacggctg ccagcgcgga tttcacgtcc ccattggcgg gaataacgtg catattgccg
     5341 ctgtgccgaa taatttcatc cacggggatc ggaggtcctt tggcgtccca acgataatga
     5401 taccaacggc catattgctc gatttccagt tttccataga tggctgaatc gctcatcgcc
     5461 ccccagccaa gcgccaggtc gtagggtgcc aattccgatt cacgaccaaa ccaatagcgc
     5521 tccttaccaa gcacccgcgc ttcgagcttc agggtcgcaa gcgcttcgaa ctcgtaatct
     5581 cccaactttt ccatcttggg agccggtaac gaaacctgca ccggatcgtt gggcgcttgc
     5641 acgcccggct cgggaaggcc gccttcacag gccatcgcgg ccaatccagc cgaaataagc
     5701 catccgcccg ccacgcaatc gctggttcgc atgccatggc ccgatcgaat tcgcctgccg
     5761 gctattcacc catcgcagga ccgctggctt cccaattctc tgccgacgtc gtcaccaccg
     5821 ctgtcgtcct gcggtattcc ttgtcgatgg gtcgcccata acacggcaac ggatcggagc
     5881 tttctgggct cttttccggg gttgggctct ctttcgacag cccggcttcg cccacagaac
     5941 gcacgatgat ataagaagga atggcctgat cggtgggaaa ccaccatgga ttccaaaccg
     6001 ggtcttgcgc ttgtttcagc aactgtacgg aaagaatatc cacccaaact tcactgacat
     6061 tcaacgcgtt gacttgcgcc aaaatatctt cgaggtccct atcgaggcat aaacaagctt
     6121 gacgaaccgc atcatcggcg tgttgcgctt gaaccacttt cgcagcggga aatgtcgctc
     6181 gatacggcat gcaactgatc cgtgtgtaac gcaaaaccag ctcgactctg aagtaccgaa
     6241 cgttttcggg ctgatgcttc acggttcttc gtcctcccgg atactgtcgt ccgaactacc
     6301 cggacccaac agcaatcctt cttgaatttc gacaccttcc gtatccacga cctcgccatg
     6361 cccgcttttt gaaaaaaact ccgccgcgcc aggcatccag ccgtgctgtt cgggggcttg
     6421 ttcccatcct tgcggatcga gggcaacgac ctgcatgacg ccttgtcgag cttttttctc
     6481 catgaccgcg cgcagacgct ctgcaaccat gcaccgaaaa cgcggaacga gcaaaagaag
     6541 accgaaaagg tccgtcaaaa aacccggaag aagcaagaaa accccggctg cgagaatgag
     6601 aaatccgctc agaataccat cgctcgggag ctgaccttcg gataacgact gccgcaccag
     6661 gctccagacc cgaattcccc cccgcttgac cagcacgcca cccagcacgc cggtgacgag
     6721 catgaggccc aaggttttcc agacgccaat ccacgatcca actgccgaca gcacgaatac
     6781 ttcgaggaca aaagccagca gcagaaaggc aaaatagcgc acgcccctac cctagccacg
     6841 acaaggtcga gttccaaagt agaacgacac caggggggag aagattttcg tttcgcttat
     6901 aagtaattat atttattgtg ctttttgggg ttcttcgaag tcgatccccc cgcgaatccc
     6961 acggctatcc cccaaccgcc cgtcgaccac gagccgccga aatccatcag tcgggtgaag
     7021 agaccgcggt cggatcacgc tggaaatagc tcacgaaggc catgatttcc cgaaagacgt
     7081 cctgggcttc catgtcttgc atcatctcgt gaccgctatg ttcgaaccaa accatttccc
     7141 gaatcggtga actgactttt tcgtaaatga tattggccga ttccggcgca atgatttgat
     7201 cctttttcga ctggagaatg cggatgggga catggacttc cggcaaacgc tcggcaattc
     7261 gccgcgaata atcgtaaagg gaagcgaaag agtccgtggc gaacatcgga tagttggtgc
     7321 aacgcttcgc caaggtcggg tcgttgaaag actcgggcga cggccagtac cggacccctt
     7381 tggaaagcag agacgtaaac atggcgagcg ggtctttgaa cttgagcgcc gccgccaccg
     7441 tgaccaaacc cgcgattttg tcgggatgac gcatggcaag ctcgagcccc accaatcctc
     7501 ccatgctcag accgaccacc accacccgat ccacgcgatt ccacaattga agaagcgcgc
     7561 ggtccgcatc cacataccag tcccgcgccg tgaccccccg catgtcctga taccgcgtgc
     7621 cgtgaccgcg cagaatcggc cgctcatagt cgatcccctg cttttcgaga aatggtagca
     7681 gcccatcgac cgtgtccgtc gaggacgtga atccatgcaa tagcagcaca ccaacctttt
     7741 cgtaggcggg cggcgcggga atgggcacct cgatgggagc gtaattgctg cggtgatcga
     7801 cgagttcgga gatgcgacgc atcaactcat cggtgagttc cctcagcact tccttatcga
     7861 cttcttggcc gtggtattgg gaataatcga tgggctcgcc gaaccgaata cggataggag
     7921 aggcgccagg catgcgcaaa acctcgagcc gtggatagat ttccggagga agcgcccgcc
     7981 ccgtgcccga cacccccacc gggacaatgg gaacacctgc gctgatggcc agtcgcaccg
     8041 cgcccgtctt gccccttagc aaacccgtcg accgatcgac tgcac
//
LOCUS       DAOSOR010000232         8073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8897_length_8073_cov_4.679346, whole genome shotgun sequence.
ACCESSION   DAOSOR010000232 DAOSOR010000000
VERSION     DAOSOR010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8073
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8897_length_8073_cov_4.679346"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1428)
                     /locus_tag="PK710_13215"
     CDS             complement(<1..1428)
                     /locus_tag="PK710_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10730.1"
                     /translation="MSRFFGLVRWALVLTAWWVASCAGLQTQPLSPTERARRDGPASS
                     DPEVVGRWLLSELISPDADFKRADRARKRLGDLGGAGMIASLARGLDAEAHGRPEPSA
                     RAYLEAWKSARTSSDPLAPVVAWYATNRILRIQNSSRWLWNDAKSFVVDSMNAPGSLG
                     WRARGELVEWWARESYREARKDMLEQVADLHGCSKHVRLAGPFGRGIPADRHRSFEAE
                     KPGRWPLFFSPSEQRPQVVPRVLETKRVGCEITPAQAVHDGVFYAETFFELQADEEVL
                     ISVQAARAVFVDDALVLERDPRKWGIWPSFGVRLKLTAGRHRLVARIEQAFTSIRLQR
                     RDGTPLHAKTSDDPSAPYSVVAPVKLADPNVLNRYIRDGDVVRPKDDVEAFLAAYLAH
                     VEGQDDVGSVLMEPLVSQIEVAAPIALAQQALLVSNDPAFPEGTGRDLARELHQKVVE
                     KDPSVWRSRHWLSVDGARKRGLAVVA"
     gene            1741..2301
                     /locus_tag="PK710_13220"
     CDS             1741..2301
                     /locus_tag="PK710_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10731.1"
                     /translation="MSKLPLSYEAYLEEEWLEKPEWITLGWTYWRRGRGVLMGIGIAG
                     FILFFLPWVKVISPESLTLSGTDLARALPWIWACPVAWLMLVTTAVSRRSVAKMRGAR
                     VATALFCAVPVVTIVVLILTPPTSRWIPIRFDYSWWMYATGLLGAMGLPFSVLFGGKL
                     NNLPLPAGVVPPAIENKPVQEPSADG"
     gene            complement(2509..4743)
                     /locus_tag="PK710_13225"
     CDS             complement(2509..4743)
                     /locus_tag="PK710_13225"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HOT10732.1"
                     /translation="MESAKTRAVLLFLLVMAFGVLLFGGYIMHKDKPPIPKEVVTASG
                     QTIYSEKDILAGQNYFYSRGGQHIGSIWGHGAYLAPDWSADYLHRMGLFVAARHLGKT
                     AEEAAAFSQTDLEQLDVGTRARITALATEEQKTNRLDAGSGRLTFTDYQAEAHAALAS
                     YYTNLFQQGNERMGLQPGIVDTAENGKAVASFFSWLAWAAGTNRIGESHTYTSNWPFD
                     PLVGNHAMPGALIWSIVSVILLILGVAGAIFLYQRYVHDDDYKSEPVTTLEEPSPTPS
                     QKATLAYFLIAIALFVIQIGMGAVTAHFTVEGTSFYGIAIGEILPYAAVRTWHLQLAV
                     FFIATCFLAAGLFIGPFVGKEPKHQGKLVWTLLAAVVVVVVGALGGTYASVKGTFGGE
                     GFWLGHQGYEYIELGRVWQVLLIVGMIIWLVLVVRAIRPALSDEKDAGGITHLLLYSA
                     IAIPAFYMVGLLYGKGSHLSDAEYWRWWVVHLWVEGFFEVFATVFMAFLLTRIGVVSS
                     RFALRAVYFSISLYLGSGVIGTFHHLYWTGAPTPIVALGAVFSALEVVPLTLVGFEAV
                     HNLRAAGTTKDNPYRWPIYFFVSVAFWNMLGAGVFGFLINPPIALYYIQGINTTPIHS
                     HTALFGVYGLLAISLMLFSMRHIVAKSAWSDKLLKFSFWGLNGGLALMTLVSLVPSGF
                     YQFYYAVRDGMWYARSPEIASGPVIRNLAYARVVPDLIFALGALLLLVFVARAILQSR
                     ASKT"
     gene            complement(4755..5408)
                     /locus_tag="PK710_13230"
     CDS             complement(4755..5408)
                     /locus_tag="PK710_13230"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT10733.1"
                     /translation="MLDAQQTPSQPLSPRQSQIVDAAFRLIATEGPRRFTVGLLAKEV
                     GLTGGAIYRHFDSMDAIVDAVVDRVGRLLFENFPPQADDPIRRLQLFFYCRARSILGN
                     PYLSRLLLSDHLLQAGRASQSQRLNEFKRQSHSFVIGCLREAQHNGSLVDEMSPEAGA
                     VVILGSVLALSHTTAKLTQESERERLFHQVWSGIERMLRRSELAMNSMHRRRPSVGK"
     gene            5572..6171
                     /locus_tag="PK710_13235"
     CDS             5572..6171
                     /locus_tag="PK710_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10734.1"
                     /translation="MANYDSLVVALESGASGSEIAAWLEGMSIQQRVRVVAAVKGPWV
                     GKLFDRVRDAEPLVIDDLVPATTPAGATITYAGRNSLPLFSRFQKRFTRSADGLVFGY
                     NHQPFRIAGKVTGPGYFVVADAGEQRPGELLFDYTQPPPFEPSGWPPYRPNDVLGARF
                     VFMDMHDYVRRVARGVVVGSAFRRGKDQNTYFSLTSIAG"
     gene            6399..7835
                     /locus_tag="PK710_13240"
     CDS             6399..7835
                     /locus_tag="PK710_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10735.1"
                     /translation="MLIDMRQRRRCVLMFTVVVTLFLLAAPSMGQQASDSDVPSDYAD
                     TDPSAVEVFREELAPYGMWVEDPDYGLVWIPSVEVVGPEFIPYMSAGYWGVTEQGDWI
                     WVSDYTWGWAVFHYGRWVWIPQRGWAWIPGRTYAPAWVVWRVGEPGYDYIGWAPMPPS
                     YYWYGGVAVSLWVIPPPYYVYCETRYVFAPHVHSHRLSGYRAHEASRHTRPHRPASPS
                     LRTDRHAARPHRSPTMEQARVPADAVPRTPATAHPRLATSRSSQPSASRLTTAPSTTS
                     GVPRARPSSSLAAVSQPVYGGMPSNALPTNRRASPLAARPMQPSASYGRMDVSRSSRT
                     LGSSSAPSSLGARPIIPRTDGHRPSAYDGQRRGDPGMVRGHSMDRGSSSRPSYSQPGS
                     TSSVGSRSSGAVTPRPQPSRVSGSMHPVRESTGGSSMRSSMSPSYRAPAASPAPAPTG
                     TTNNTYKQGGSSKGRGSVSGHGPRGGRR"
     gene            7862..>8073
                     /locus_tag="PK710_13245"
     CDS             7862..>8073
                     /locus_tag="PK710_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10736.1"
                     /translation="MFDAFLTYGERILRVSHPSCWGGYALVTAVFLSVSGCIIESGPC
                     DECDCNENGDGSGGYAGGGNGYGGTGG"
BASE COUNT         1648 a         2198 c         2498 g         1729 t
ORIGIN      
        1 cgcgaccacg gcgagtcctc gtttccgcgc accatcgacg ctcaaccaat gtcgagagcg
       61 ccagacactc gggtcttttt ccacgacttt ttggtgtagt tcccgcgcca aatcgcgacc
      121 ggttccttcg gggaacgctg ggtcattgct caccagaagc gcttgctgag ccagtgctat
      181 cggagcagcg acctcgatct gagacaccaa aggctccatc agcacgctgc ccacgtcgtc
      241 ttgcccttct acatgcgcca agtaagccgc gagaaacgcc tccacatcgt ctttgggccg
      301 caccacatcc ccatcgcgaa tatatcgatt caacacattg ggatcggcga gtttgacagg
      361 cgcaacgaca ctgtagggtg ctgaaggatc atcactggtt ttggcgtgta gcggcgtgcc
      421 atcacggcgt tgaaggcgaa tggaagtgaa tgcttgttcg atgcgcgcga ccagacgatg
      481 tcttcccgcg gtcagtttca agcgcacgcc aaaagaaggc cagatacccc attttcgggg
      541 atctcgttcg agcaccaaag catcatcgac gaaaacggct cttgcggctt gcaccgagat
      601 gagcacttct tcgtcggctt gcagttcgaa aaaggtttcg gcgtaaaaaa cgccatcatg
      661 gacggcctgc gcgggggtga tttcacatcc gacgcgtttc gtttccaaca cgcgaggaac
      721 cacctggggc cgctgttccg agggtgaaaa aaataggggc caacgacctg gtttctccgc
      781 ttcgaaggag cgatggcgat cggcggggat acctcgacca aacggacctg ccaatcgcac
      841 gtgtttcgaa caaccgtgca agtccgcaac ttgctcgagc atatccttgc gcgcctctcg
      901 ataagactcc cgagcccacc attccaccaa ctcgcctcgc gctcgccatc cgagggaacc
      961 gggcgcattc atcgaatcca cgacgaaaga cttggcgtcg ttccatagcc atcgactgga
     1021 gttttgtatt cggaggattc ggttggtggc ataccatgcc accacgggtg ccaacggatc
     1081 gctcgacgtg cgagccgact tccacgcttc gagatacgcg cgggccgaag gctcaggacg
     1141 cccatgcgct tcggcgtcca gcccgcgggc caaggaggca atcattccgg ctcccccaag
     1201 gtcgccgaga cgctttcggg cacggtccgc gcgcttgaag tcggcatccg gagagatgag
     1261 ttccgacagc agccaccgtc caacgacttc ggggtcggag gaagccggac catcgcggcg
     1321 cgcccgttcg gttggcgaaa ggggttgggt ttgcagcccc gcgcaggatg caacccacca
     1381 tgctgtcagt accaacgccc atcgtaccag cccaaaaaat cgactcatgc cagcacgtgc
     1441 ctaccgcagc ccggctccga ccgcatctgc ccaagtgaaa gaagtgccat tttgttcgac
     1501 ctgcctcatc gctttcgagc gctttacgct tcgtttcatg gcaaatcggc aatttctttg
     1561 tggccaggcc gtttgggtcg gttggtacac cgcacggctt tgccggattc gccatcacag
     1621 aaaaaagtaa gctacactgc cggtgtcttg agcgaccttc ttgcctgccc cttttgccgt
     1681 gagcttttct ccaaggaaga gactcgaacc tgtcctacgt gcgggctccc gctctctccc
     1741 atgtcgaagc tgccgctatc ctacgaagcg tatctcgaag aagaatggct cgaaaaaccc
     1801 gaatggatca cgctcggctg gacttactgg cgtcggggac ggggtgtgct catgggcatt
     1861 ggtatcgctg gattcatcct gtttttcctt ccctgggtga aggttatctc ccccgaatcc
     1921 ttgactcttt ccggaaccga tctcgcgcgt gcattgccgt ggatctgggc ctgtcccgtg
     1981 gcctggctca tgctggtgac aacggccgtg tcgcgtcgat ccgtggccaa aatgcgtgga
     2041 gctagggtcg ccactgctct gttttgcgca gtaccggtcg tgaccatcgt cgtgttgatc
     2101 ctgacgccac cgacgagccg ttggattcct attcgtttcg actattcctg gtggatgtat
     2161 gccacggggc tgcttggcgc gatgggactt cctttcagtg tgctgtttgg cggcaagcta
     2221 aacaacttac ccttgcctgc gggtgtggta cctccagcca ttgaaaacaa acctgtgcaa
     2281 gagccatcag cggatgggta accagggacc gcgtaggata aaacctgaat aattaccata
     2341 agttatgact atgcggtccc ctgactggcg tggagaatca agcggggcca tcaagggctg
     2401 cacgatgggg cgatgacgac gatttcaggg caagagtccg tgatcatcgt tgggcaggaa
     2461 gggaaagtgg ggagggaaaa acggtgggcg aatcgttcga cagaccgatc aggttttgct
     2521 ggcgcggctt tgcaagatgg ctcgtgctac gaaaaccaga agcagcaggg cgcccaacgc
     2581 gaagatgagg tcgggaacga cgcgtgcgta ggcgaggttg cggatgacgg gaccggaggc
     2641 gatttcgggg cttcgggcgt accacatgcc atcacgcacg gcgtagtaga attggtagaa
     2701 gccggagggc acgaggctca cgagggtcat gagagccagt ccgccgttga ggccccagaa
     2761 gctgaacttc agcaatttgt cggaccaagc ggacttggcc acgatgtggc gcatggaaaa
     2821 gagcatcagg gagatggcga gcaagccgta cacgccaaat agggctgtat gcgagtggat
     2881 cggcgtcgtg ttgatgccct ggatgtaata cagcgcgatg ggaggattga tgaggaagcc
     2941 gaacacgcca gcgccgagca tgttccagaa cgccacggag acgaagaagt agataggcca
     3001 gcggtaaggg ttgtctttgg tggttccggc ggcgcgcaag ttgtggacgg cttcgaagcc
     3061 gacgagggtc aggggcacga cttccagtgc ggagaagacg gcgccgagag cgacgatcgg
     3121 tgtgggtgcg cctgtccaat acagatggtg gaaggtgccg atcacgccgc tgccgaggta
     3181 aagggagatg ctgaagtaca cggcgcgaag tgcgaagcgg gagctaacca cgccgatccg
     3241 cgtgagcaaa aacgccatga acacggtagc aaagacctcg aagaagcctt ccacccacag
     3301 gtgaacgacc caccagcgcc agtactcggc atcgctcaag tggcttcctt tgccgtaaag
     3361 caggcctacc atgtagaagg cgggaatggc gatggcgcta tacagaagca agtgggtgat
     3421 gccgccagca tctttttcgt cggaaagggc gggacgaatc gcacgcacga ccagcacgag
     3481 ccagatgatc atgccgacga tgagcagcac ctgccagacg cgtcccaatt cgatgtattc
     3541 gtagccttgg tgtccgagcc agaagccttc accaccgaag gttcctttga cggaggcgta
     3601 ggtgccaccg agggcgccaa cgacgaccac gacgacggcg gctagcagtg tccacacgag
     3661 tttgccttga tgttttggct ctttgcccac gaacgggccg atgaacaggc cggcggcaag
     3721 aaagcaggtg gcgatgaaga acacggcgag ttgcaagtgc cacgttcgta cggccgcata
     3781 aggaaggatt tcgccaatcg caatgccgta aaagctcgta ccttcgacgg tgaagtgggc
     3841 ggtgacggcg cccattccga tttgaatcac gaatagcgcg atcgcgatca agaagtaggc
     3901 gagcgtagct ttttggcttg gtgtcggcga aggctcttcg agggtcgtca ccggttcgct
     3961 cttgtaatcg tcgtcgtgaa cgtatcgttg gtagaggaaa atggcgccgg ccacaccaag
     4021 gatgagcaag atgacgctca cgatggacca gatgagagcg ccaggcatgg cgtggttgcc
     4081 cacgagcggg tcgaagggcc agttactggt gtaggtatgg gattcgccga tacgattggt
     4141 gccggcggcc caggcgagcc aggagaagaa gcttgcgacg gcttttccat tttcggcggt
     4201 atcgacgata ccgggctgaa ggcccatgcg ttcgttaccc tgttggaaca agttggtgta
     4261 gtaggaggcc agggctgcat gggcttcggc ttggtagtcg gtgaaagtca gccgtccgga
     4321 gcctgcatcc aagcggtttg ttttttgctc ttcggtagcg agggctgtga tgcgagcgcg
     4381 cgttccgacg tccagttgtt cgaggtcggt ctgggaaaac gcggcggctt cttcggcggt
     4441 tttgccaagg tgtcgagctg cgacgaacag ccccatgcga tgcaggtaat cagcagacca
     4501 gtcgggtgcg agataagcgc cgtgacccca gatgctgcca atatgctgcc cgcctcgact
     4561 ataaaaatag ttctgaccag cgaggatgtc tttttccgag taaatggtct ggccgctggc
     4621 ggtgacgacc tccttgggga tgggaggttt gtccttgtgc atgatgtatc ctccgaagag
     4681 aagtacgcca aaagccatga cgagtagaaa cagcagaacc gctctcgttt ttgcgctttc
     4741 catcaatatc tcctttattt tccgacgctg ggacggcgtc gatgcatcga attcatggcc
     4801 agctcggagc gtcggagcat tcgctcgatt ccggaccata cttggtggaa gagacgctct
     4861 cgttcggatt cctgggtgag tttggcggtc gtatgagaca aggcgaggac ggagccgagg
     4921 atcacgacgg cgccggcttc gggactcatc tcgtcgacca acgagccgtt gtgttgtgct
     4981 tcgcgaaggc aaccgatgac gaaggaatgg gattgccgtt tgaattcgtt gaggcgctgg
     5041 gattgggacg cgcgacccgc ctgcaacaag tgatcggaca gcagcaaccg ggaaagatag
     5101 ggattgccga gaatcgagcg ggctcgacag taaaagaaaa gctggagtcg tcggatggga
     5161 tcatcggctt ggggagggaa attttcgaat agaaggcgtc ccacacgatc cacgacggca
     5221 tcgacgatgg catccatgct atcgaaatga cggtagatgg ctccgccggt gaggcccact
     5281 tccttggcaa gaagtcccac ggtgaaacgt cgtgggcctt cggtggcgat cagccgaaac
     5341 gccgcatcga caatttgtga ttgccgtggg gaaaggggct gggatggagt ctgttgggcg
     5401 tccagcattg gcggcggagg ctatgtgaat aaatatttac ttacaagctc tatgatcact
     5461 ttttctttct tcctgtgtaa atctctaaaa aatcgacaat ttagtatata tgaaattata
     5521 tactcaaatc ggaagccgca tttttgtgtg ttggcggggt atgatggggg gatggcgaat
     5581 tacgattctc tcgtcgtggc gctcgagtcg ggagctagcg gttctgagat tgcggcatgg
     5641 ctcgagggca tgtcgataca gcagcgggtg cgggtggtgg cagcggtgaa agggccttgg
     5701 gtaggaaaac tattcgaccg ggttcgcgac gccgagccgc tcgtgattga tgaccttgtg
     5761 ccggccacga caccggcggg tgcgacgatc acgtatgcgg gacgcaactc gcttcccttg
     5821 ttctcacgtt ttcaaaaacg attcacgcgt tctgccgacg gactggtatt tggttacaat
     5881 caccaacctt ttcgcatcgc cggcaaagta accggacccg gatacttcgt cgttgcggat
     5941 gcaggcgaac agcgtcctgg agagttgctt ttcgactaca cccaaccgcc ccctttcgaa
     6001 ccgtcgggat ggcctcctta tcgacccaac gatgtcttgg gcgcgcgttt cgttttcatg
     6061 gacatgcatg actatgtccg acgtgttgcg cggggagtgg ttgtgggctc ggcatttcga
     6121 cgaggcaagg atcagaatac ctacttcagt ttgacgtcga tcgcgggttg agcggtgtct
     6181 gttttctcac gggccccacg ctccctaccc attcaatcta tccgtatttg tatactcaaa
     6241 tcacccgtat cacgtagtat ccttgggttc gtatccttcg ggaccgtcga tggtattcgt
     6301 ttggctacct cccttacccc gtgaaaaccg atctgtggcg agctacggaa tctccaatgg
     6361 catggcgctt gccctggcat cgagacagga gacggttcat gctaattgac atgaggcagc
     6421 gacgacgatg tgttctgatg ttcacagttg tggtgacact ttttcttcta gccgctccat
     6481 ccatgggtca gcaagcgtcg gacagcgatg tgccgtcaga ctacgctgac acggatccgt
     6541 cggcagtcga agtgtttcgg gaggagctgg cgccctatgg aatgtgggtg gaagaccctg
     6601 attacgggct cgtttggatt ccgagtgtcg aggtggtagg cccggagttc atcccttata
     6661 tgagcgctgg gtattggggt gtgacggagc aaggggactg gatttgggtg tctgactaca
     6721 catgggggtg ggcggttttc cactacgggc gctgggtttg gataccgcaa cggggttggg
     6781 cgtggatacc tggacgaacc tacgcgccag cttgggtagt atggcgcgtg ggggaaccgg
     6841 gatacgatta cattgggtgg gcgccgatgc cgccatcgta ctattggtac ggtggggtcg
     6901 ccgtatccct ctgggtcatt cccccgccct attacgtcta ttgcgagaca cgttacgtgt
     6961 ttgctccgca cgtccattcc catcgtcttt ccggataccg agcgcatgag gcttcgcgcc
     7021 acacgcgtcc ccatcgaccc gcgtcgccgt cgttacgcac cgaccgtcat gccgcaagac
     7081 cccatcggtc tccaacgatg gaacaagcac gcgttcccgc ggacgctgtg cctcgcacac
     7141 cagccactgc gcatccgcgg ctcgcgacat cacgatcgtc gcagccgtcc gcttcgcgtc
     7201 tgacgaccgc gccatcgacg acatcgggag tccctcgcgc cagaccatct tcttctctcg
     7261 cggcggtgag ccaacccgta tacgggggca tgccatcgaa tgcgttgccg accaatcgga
     7321 gagcttcgcc cctggcggcg cgccccatgc agccgtcggc atcgtacgga aggatggatg
     7381 tctcgcgttc ttcccggacc ttggggtctt ccagcgcgcc ttcctcgttg ggtgcccgac
     7441 ccatcattcc tcgaacggat gggcaccgtc cttccgctta cgatgggcaa cggcgtgggg
     7501 atccgggcat ggttcgtggt cattccatgg accggggttc atcgtctcga ccctcgtatt
     7561 cacaaccggg atcgacgagt tccgtcggat ctcgatcgtc gggagcggtg acgcctcgtc
     7621 cgcagccgag tcgtgtttcc gggtcgatgc atccggtcag ggagtcgacg ggggggtcat
     7681 cgatgcgttc ttcgatgtcg ccttcctatc gagcgccggc agcatctccg gcgcctgccc
     7741 ccacgggtac gacgaacaat acctacaaac agggtggaag tagcaaaggt cgcggttccg
     7801 tatcgggtca tggtccccga ggcggccgtc gctagttgag tgcaacatcg gaggagctac
     7861 catgttcgat gcatttctta cttatggtga gcgtattttg agggtgtcgc atccatcttg
     7921 ctggggtgga tacgccttgg taacggcggt ttttctgagt gtatccggct gcatcatcga
     7981 atccggtccc tgtgatgaat gcgattgtaa tgaaaatggc gatggttcgg ggggatatgc
     8041 tggagggggg aatgggtatg gcggcacggg tgg
//
LOCUS       DAOSOR010000233         8057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8922_length_8057_cov_3.988628, whole genome shotgun sequence.
ACCESSION   DAOSOR010000233 DAOSOR010000000
VERSION     DAOSOR010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8057
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8922_length_8057_cov_3.988628"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PK710_13250"
     CDS             <1..498
                     /locus_tag="PK710_13250"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT10737.1"
                     /translation="FAQSLPAASRQMKTLAGKLCAKVLDFGMARLLWTARETDPSLAL
                     GTPVYMAPEQLMNYNVTFAADIYSSGITLYQMLTGQPPFVGNNLREVAKHILRGKLPD
                     VRTFRPDIPADLVTVLSKSTALLVENRYTSARHFRHELYAVGKKLKLARNLMKDSRPW
                     WKKLV"
     gene            551..1381
                     /locus_tag="PK710_13255"
     CDS             551..1381
                     /locus_tag="PK710_13255"
                     /inference="COORDINATES: protein motif:HMM:NF012369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT10738.1"
                     /translation="MEAIAIYGKGGIGKSVVATHLSAELARRGLRVLHVGCDPKADSS
                     CRLVGSHHIKTVLDAMRKDPDDIVESEFLKPGRLGIDCIEAGGPEPGSGCGGRGVARA
                     IEIICELNMLDRGTYDAAVFDVLGDVVCGGFAAPMRKGVANKIYIVISEESMALFAAN
                     NIARAVLANASNGVVLAGLIANLRSSDEAAHARVQRFAQRIGSTIVMILPRSPLIANA
                     EQYLLTAVEQYPDSDIAISFQRLATHCLSASPTSQTALTPMEQEEFFQFLQEGPSVAS
                     "
     gene            complement(1413..2528)
                     /locus_tag="PK710_13260"
     CDS             complement(1413..2528)
                     /locus_tag="PK710_13260"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT10739.1"
                     /translation="MNDLDPWLTQASVWTCVRRLARGQPCPSPLSIELHLGTACNLAC
                     PECISRPILDTGRFEPRRAISLAQEIVDLGTRSVVLSGGGEPLLHPSVDEVLRTLSEG
                     GVASALVTNGTLLSRHFDAVVKYPQRVWISVDAATARLYSLFRPSRHGAPSVPFDKMI
                     DDIGRLASARRGNVAFSFLLLGRDTERGIVTNATELAQAARIARDLGCKTFEVNVGIG
                     RDMLPISSLRSVSSIVSEQLASLRTLVRQDFHIHVSPWVESVARGESLELLGKRRGER
                     CRAAEVRALVSPRGVYLCAQQLDEEFARYGDPTQNTLREIWQSPVRQDAMDRTMQHSA
                     CDSCLSMGLQERLSQGISYVREGAMEPIIHPDEDWFL"
     gene            complement(2525..3931)
                     /locus_tag="PK710_13265"
     CDS             complement(2525..3931)
                     /locus_tag="PK710_13265"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOT10740.1"
                     /translation="MTVDESAKEASTDDLSVRAALPRTVGSTVAINAVPDAYLLIDSP
                     HCTYRRIAYVQGNHDYMSTLAWYPDTPRVTNTELTPLDVIRNRDEAIVRELLSLTEHP
                     QAGVVLVDAMAMALITATDYDRLCARAKEKTTKPVLRVPHRSLTSDHLTGYADVMEAL
                     ASGLPLHPNPSKTGNRVGLVGYYWDRNEGDHRGNMTELERIADALGLEWTTPWLSGSP
                     SSALRDIENAQWIVSLPYGRKAAQILAKRLGCPLLRAEYPFGLAASLRFIVALGVMTG
                     RLSAALTFVQAELETILPRLAWVVPFALLHRRIGFLGDPHLVLGMQEMASLWGCTMPF
                     AATSCLPRHARDSAPDYGNALHDPSIPEDVRTLMNIDQPVDAPSNFLSYPHVRELVRE
                     LEHLAQEQRLDVLVTNSLGFLTKGIGIVEFGFPSYHTHALIDRPFVGLLGFLTMMERV
                     VNALRAHEVQMAQSSGGV"
     gene            complement(3918..5726)
                     /locus_tag="PK710_13270"
     CDS             complement(3918..5726)
                     /locus_tag="PK710_13270"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOT10741.1"
                     /translation="MTPGVPMPTEAIVCGAFTRERVERLLGLDIEPITYRIEKAQWTH
                     GRLEIVVRPVDEPADDIELVLEQPSPQQQAFLRGPEVCLWARQRTLPPALARRLTRLA
                     SQRLARLRFSDLLKVIHLDPDVERASHQSEAPAPDGISTASVMTQAHTHPSLFADFFA
                     ASEFRRSRGDMLDVFSAHSLVCHGDTECAYCSVVAPRPRNALVRLPVLETIRAIGRWH
                     QSTKGFNESPDITDITCSDMTEQDVILGAPGKLTAALIDADRKRKRDALLVIGLCVPD
                     VIGENEDAIVQSYRKTTSTPVLTVPASPRSWGWLVQNMLETRRAPHDHDSPPEPNRIN
                     LIGFGDNPGVREIYNLLKIQGIIVNRHVLPRLAMVAIRDLHRATMNVYLPNEYWERAY
                     SYLKQDAGRIHLQCDGPYGIAGTKRWLESIHHALGMPASEVVGCKDVLLERWEQLRAQ
                     AKQHRLGLVVPWRERNTLTDSRKTWGIPLVDFLTEMGFGIDIFVATEGSDSVDDMDFW
                     DSPETSVTIVHSLHELLQSLERNLCEAVFSNYVFDWRLVRSGKMPFSTLEMQMGYEGS
                     VETLRRLLAVCRTPLYRAGRRFMQGVGPNTYRHDSG"
     gene            complement(5986..6831)
                     /locus_tag="PK710_13275"
     CDS             complement(5986..6831)
                     /locus_tag="PK710_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10742.1"
                     /translation="MSSHLLSARAPGDGADRPDATHQDRRYVYVARSRRAGGLSLGID
                     LTPEGHCSFSCIYCQASHPPTSHPELAVDVPRMREDLRLRLQQDNGELKDIVLAGSGE
                     PTMVPNLADALEAIVDVRDELRCPLPIKLFTNGRHLGKDAVHQAVGRFAQRGGQVWIK
                     LDGASEETLATINGRRFAVQEHLRDLWQFAKQHPVGLQTLLCHGPGLPDPSHVVEEVT
                     HAIAEGLSQGARITEIHLLTLSRRPADERAASRLQPVPLASLEEFALQIQKVISIPIL
                     VFPSA"
     gene            6939..7442
                     /locus_tag="PK710_13280"
     CDS             6939..7442
                     /locus_tag="PK710_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10743.1"
                     /translation="MTSPCRCPDLRCADTEHILPATDEEQVLMVVEVGLVAAMMDNAI
                     PESESTALVTAIRMLPGMRNLSDSDVNLVLSRAGTRAQEGDIWLCDVSRRLTHPGLRR
                     VAFRMASMFCAWDGVIDDKEQGYLNFLATVFEFSPAEADALFAQATGQRGSASVVPPP
                     AEMSETG"
     gene            7548..>8057
                     /locus_tag="PK710_13285"
     CDS             7548..>8057
                     /locus_tag="PK710_13285"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HOT10744.1"
                     /translation="MQAPTKKVLPENAYRKLEPGEVYEPIVPHDDNRAEVTVWSLSLG
                     VVMVALFAAAGIYMALRAGNAIETAIPIAIMAVFFGKLNRVRSTILENVMVQSIGQAS
                     GVVAAGAAFVIPALYINQLQPSFWHIFFATAIGGFLGVVLIIPLRKYFVADQHGELPF
                     PEATATNEIL"
BASE COUNT         1768 a         2346 c         2238 g         1705 t
ORIGIN      
        1 ttcgcacaga gcttgcccgc agcatcacga cagatgaaga cgttggcggg caagctctgt
       61 gcgaaagtgc tggactttgg gatggccaga ctcctttgga ccgcgagaga aaccgatccc
      121 tccctggcgt tgggaactcc cgtgtacatg gcgcccgaac aattgatgaa ctacaacgtc
      181 acgtttgcgg ccgatatcta cagttcgggt attacgttgt accagatgct gaccggtcaa
      241 cctcctttcg ttggcaacaa tctgcgcgaa gtcgccaaac acattctccg cggtaagctg
      301 cccgacgtgc gcaccttccg tcccgacatc cccgccgatc ttgtcacggt cttgtccaaa
      361 tccaccgccc tgcttgtcga aaaccgttac accagcgcca ggcactttcg gcatgaattg
      421 tatgccgtcg gaaaaaaact caaacttgca cgaaacctca tgaaagacag tcgtccttgg
      481 tggaaaaaac tcgtttgaac catctgatcc atcgcaagac ttcgtcgttc gccccatatc
      541 atccagcatc atggaagcca tcgcaatcta cggaaaagga ggcatcggaa aaagcgtcgt
      601 ggccacgcac ttgagcgcgg agttggctcg tcgtggactt cgcgtcttgc atgtcggctg
      661 tgaccccaaa gccgattctt cctgtcgttt ggtgggaagc caccacataa aaaccgtgct
      721 cgatgccatg agaaaagatc ctgacgatat cgtcgaaagc gagtttctta agccaggacg
      781 gcttggcatc gattgtatcg aagccggtgg gcccgagccg ggatctggat gtgggggtag
      841 gggagtcgct cgcgccatcg agatcatctg cgaattgaat atgctcgacc gcggaaccta
      901 tgacgccgct gtcttcgatg tactcggtga tgtcgtttgc ggcggatttg ccgcccccat
      961 gcgcaaaggc gtcgccaaca aaatctatat cgtcatctcc gaagaatcca tggcgctatt
     1021 tgccgcaaac aatatcgctc gcgccgtgct tgccaatgcc tccaatggcg ttgtgctcgc
     1081 cgggctcatc gccaacttgc gatcctcgga tgaggcagcc cacgcccgtg tccagcgatt
     1141 tgcacaacga atcggttcga ccatcgtgat gatcctgcca agaagtcccc tgatcgccaa
     1201 cgccgaacaa tatctcctta ccgctgtcga gcaatatccc gattcggata ttgccatcag
     1261 tttccaacgc ctggcaacgc attgcctcag cgcctccccg acttcccaaa ctgcgcttac
     1321 gcccatggaa caagaggagt tttttcaatt tctgcaagaa gggcccagcg tggcttcctg
     1381 actatcacag cgacatcgtt ggcaacacaa ttctacagga accaatcctc atccggatgg
     1441 atgatcggtt ccatggcacc ttcgcgaacg tacgatatcc cttgggacaa cctctcttgc
     1501 agccccatcg acaaacagga atcacacgct gaatgttgca tcgttcgatc catcgcgtct
     1561 tgccgaaccg gactttgcca gatctcgcgc aacgtgtttt gcgtgggatc cccatacctt
     1621 gcaaactcct catccaactg ctgcgcgcat agatacacac cacgagggct caccaatgcc
     1681 cgcacctccg cggctcgaca acgctccccc cttcgtttgc ccagcaattc caaagactcg
     1741 cctcgcgcaa cgctctccac ccatgggctc acatgaatgt gaaaatcctg tcgtaccagg
     1801 gtccgcaaag acgcaagctg ctccgaaacg attgacgaaa ccgaccgcag cgaggagatc
     1861 ggaagcatat cgcggccgat gcccacattg acctcgaacg tcttgcaccc aagatcgcgc
     1921 gcgatccgtg cggcctgcgc caactccgtg gcgttcgtca ctatcccgcg ctccgtatcg
     1981 cggccaagaa gcagaaacga aaacgctacg tttcctcgcc gtgccgacgc aagcctgcca
     2041 atgtcgtcta tcattttgtc gaaaggaacc gaaggcgcgc catgccggga cggtctaaat
     2101 agtgaataaa gtcgcgctgt tgcggcatct acggagatcc ataccctttg tggatacttc
     2161 accaccgcat cgaagtgccg agacagcagc gtgccgttgg tcaccaacgc gctcgccacg
     2221 cctccttccg aaagagtccg aagaacctcg tcgacactcg gatgcaatag cggttctcct
     2281 cccccggata gcacgacgct ccgcgtgccg agatcgacga tctcctgggc cagcgaaatc
     2341 gctcgtcgag gctcgaatcg ccccgtgtcc agaatggggc ggctgatgca ttccggacac
     2401 gcaagattac acgctgtacc gagatgcaac tcgatgctta gcggcgaagg gcagggctgc
     2461 cctcgggcaa gacgacgaac gcacgtccat accgacgcct gggttagcca cggatcgagg
     2521 tcgttcatac ccctcccgaa gactgcgcca tctgcacctc atgcgctcga agggcgttga
     2581 ccacgcgttc catcatcgtc aagaaaccga gcaaacccac gaacggccga tcgatgagcg
     2641 catgggtatg gtaggaagga aaaccgaatt cgacgatgcc tatccctttc gtcaaaaatc
     2701 ccaggctatt ggtgaccaac acgtcgagcc gttgttcttg cgcaagatgc tcaagctcgc
     2761 gaacgagttc ccgtacgtga ggataggata agaagttcga aggagcgtcc acgggctgat
     2821 cgatgttcat gagcgtgcgc acatcctctg ggatggatgg gtcatgcagg gcgttgccgt
     2881 agtctggcgc ggaatctcgc gcgtgccgag gcaaacacga cgtggccgcg aacggcatgg
     2941 tgcatcccca tagggaagcc atttcctgca ttcccaatac gagatgggga tcgcccaaaa
     3001 aaccgattcg tcgatgcagc aaggcgaagg gaactaccca cgcaaggcgc ggcaaaatcg
     3061 tttcgagttc cgcttgcacg aacgtcaatg ccgcggacag tcgtcccgtc atgaccccaa
     3121 gtgccacgat gaatcgaagc gaagccgcga gaccaaaggg atactccgcc cgcagcagcg
     3181 gacaaccaag acgcttggcg aggatctgcg ccgctttcct gccataagga agcgatacga
     3241 tccattgtgc gttttcgatg tctcgaagcg cggatgatgg cgagccggac agccaaggcg
     3301 ttgtccattc gagccccaac gcatcggcga ttcgctcaag ttctgtcata ttaccgcgat
     3361 ggtccccttc gtttcgatcc caatagtacc ccaccaagcc cacgcgattg ccggtcttcg
     3421 agggattggg atgcaacggc aacccggacg caagcgcctc catcacatcg gcatatcccg
     3481 ttagatgatc gctcgtcagg gaacgatgcg gaacacgaag cactggcttc gttgtcttct
     3541 ctttcgcccg agcgcaaaga cgatcgtagt cggttgctgt gatgagcgcc atcgccatgg
     3601 catcgaccaa caccacaccc gcttgcggat gctccgtcaa cgacaacaat tcccgtacga
     3661 tggcctcatc tcgattgcga atcacatcga gtggcgtcaa ctccgtgttg gtcacccgcg
     3721 gcgtgtccgg ataccacgca agcgtcgaca tataatcatg attgccttgc acataagcaa
     3781 ttcgacgata cgtgcaatgc ggggagtcga tgagcagata tgcatccggc accgcgttga
     3841 tcgcaaccgt ggaacccacc gtgcgcggca aagccgcacg aacggaaaga tcgtccgtcg
     3901 aagcttcctt ggctgattca tccactgtca tgacgatagg tgttcgggcc tacaccctgc
     3961 ataaaacgcc ggcctgcccg atacaacggc gtgcgacaca cagccaacaa tcggcgtaac
     4021 gtttccacgg agccctcgta gcccatctgc atctcgaggg tcgaaaacgg catctttcca
     4081 cttcgaacga gcctccaatc aaaaacatag ttggagaata ccgcttcaca taagtttcgc
     4141 tccaacgatt gcagcaactc atgcagcgag tggacgatcg tcaccgacgt ttctggggag
     4201 tcccagaagt ccatgtcatc gacgctgtcc gatccctcgg tggccacgaa aatgtcgata
     4261 ccgaacccca tctccgtcag aaagtccacg agcggtatac cccacgtttt tcggctgtcc
     4321 gtcagcgtat tgcgctctcg ccacggaacc acgagcccga gacggtgttg cttggcttgc
     4381 gcgcgcagtt gttcccaccg ttccaggagt acgtctttac atcccacgac ttctgaagcc
     4441 ggcataccga gcgcatgatg gatggattcc agccaccgtt tcgttccggc gattccataa
     4501 ggcccatcac attgcagatg aatgcgcccc gcatcttgtt tcaagtaaga gtaggcacgc
     4561 tcccaatact cattcggaag atagacgttc atcgtcgcgc gatgcaggtc acggatcgcg
     4621 accatcgcga gccgggggag cacgtggcga ttaacgataa tcccttgtat tttcaaaagg
     4681 ttatatatct cccgtacccc cggattgtcc ccgaatccaa tcaagttgat acggttcggt
     4741 tcgggcgggc tatcgtgatc atgaggagcc cgtcgcgttt ccagcatgtt ctggaccagc
     4801 caaccccatg atcgagggga agccggaacg gtcaataccg gtgtggatgt ggtctttcga
     4861 taactttgga cgatcgcgtc ttcgttttcg ccaatgacgt cgggaacaca taggccgatg
     4921 accaacagcg catcgcgttt cctttttcgg tctgcatcga tcaaggcggc ggtgagtttg
     4981 cctggcgcgc ccaagatcac gtcttgttcg gtcatatcag agcaggtgat atcggtgatg
     5041 tccggagatt cgttgaaccc tttcgtgctt tggtgccacc gtccgatggc gcggatcgtt
     5101 tcgaggacag gcaaacgcac caaggcattg cgcggacgag gcgcgaccac ggaacaatac
     5161 gcgcattccg tatcgccgtg gcaaaccagc gagtgcgcgc tgaagacatc gagcatgtcc
     5221 ccccgcgagc ggcgaaactc cgaagcagca aaaaaatccg cgaatagcga tggatgggta
     5281 tgcgcctggg tcatcaccga tgctgtcgaa atcccatctg gcgcaggtgc ttcgctttga
     5341 tgggaagctc tttccacgtc cggatccaga tgaatgactt tgagaaggtc ggaaaatcgg
     5401 agcctggcaa gccgttgcga tgccagccgc gtgagtcgtc gagcgagcgc agggggcaag
     5461 gtccgctgac gcgcccacaa gcacacttcc ggtcctcgaa gaaaggcttg ttgctgtggt
     5521 gagggctgtt ccaacaccag ctcgatatca tccgcgggtt cgtcgaccgg ccgcacgacg
     5581 atctcgaggc ggccatgggt ccattgcgcc ttttcgatgc ggtaggtgat gggctcgata
     5641 tccaacccca gcaatcgttc cacccgttca cgggtgaatg cgccacacac gatagcttcg
     5701 gtgggcatgg gcacgccggg ggtcatgctc cttgcgtgcg ctacccatct cacgaagtca
     5761 agtcatcgtg gcggttcgag ggtataggac cgaccgtgcg gagccttcgg cgaaggctga
     5821 aggaaaagcc acgggggtcg aaccgaggcg agatgggccg ggacaaaggg gcctggagtt
     5881 gacctcggat cttgacgttg agattgaggc aaccttggag gccaccaagc cggtcgaatc
     5941 acatccgcgc tgagttcatg gtttcgggat tgtcgagacc agcgttcaag ccgatggaaa
     6001 tacaaggatc ggaatcgata taaccttttg aatttgcaaa gcaaattcct caagcgaggc
     6061 aagcggaaca ggctgcaagc gcgatgctgc tcgttcatcc gctggacgtc ggctcagcgt
     6121 gagtaaatga atttcggtga tgcgtgcgcc ttgagacaat ccttccgcga tagcgtgcgt
     6181 gacttcttct accacgtggc ttggatcggg cagtcccggt ccgtggcata acagcgtctg
     6241 caatcccacc ggatgttgct ttgcaaactg ccatagatcg cgcaaatgtt cttgcacggc
     6301 gaagcgccta ccgttgattg tcgcaagtgt ctcttcgctg gcaccgtcga gtttgatcca
     6361 tacttgcccg ccgcgttgtg caaaacgccc gacagcttgg tggaccgcat cctttccgag
     6421 atgccgacca ttggtaaaca gcttgatggg tagggggcag cggagttcgt cgcgcacatc
     6481 gacgatcgcc tccaaggcat cggcgagatt gggcaccatc gtgggttctc cggagccagc
     6541 cagcacgatg tctttcaatt ccccattgtc ctgttggaga cgaagccgta ggtcttcacg
     6601 catgcgcggg acatcgacgg ccagttcggg atgggatgtg ggcggatgcg aagcctggca
     6661 ataaatacac gaaaagctgc aatgtccctc gggtgtgaga tcgataccca gcgacaggcc
     6721 tccggcacga cgagaccgcg ccacgtagac gtatcgacgg tcttggtgcg tggcatcggg
     6781 ccgatccgcg ccatcgccgg gcgcgcgggc ggagagaaga tgagatgaca tggcaaccgg
     6841 gtgtctaatg cgctacagca tcgttcgcca gagacattga agccaacgtg aacttcctgt
     6901 tgcgagcgtg atgcatgtcc gccaacctga cgcaaacgat gacgtcccct tgccgctgtc
     6961 ccgatcttcg ctgcgcggat actgagcata ttctccctgc caccgatgaa gagcaggtgt
     7021 tgatggttgt cgaagtgggg ttggtggcgg cgatgatgga taatgcgatt ccggaatctg
     7081 aatcaaccgc tctggtgacc gcgattcgga tgctgcccgg gatgcgcaat ttatccgata
     7141 gcgacgtgaa tctggttctt tcgcgagcgg gaacgcgcgc tcaggaaggc gatatctggc
     7201 tttgtgatgt gagccgtcga ttgactcatc ctgggttgcg gcgtgtggcc tttcgcatgg
     7261 catcgatgtt ttgcgcgtgg gatggggtca tcgatgacaa agagcaggga tacttgaatt
     7321 ttttagcgac agtattcgaa ttttcacctg ctgaggctga tgcattgttt gcgcaggcta
     7381 ctggacagag gggttcggcg agtgttgttc ctcctccggc ggagatgagc gagacaggtt
     7441 gattcgcgca attcccagaa atacttcacc agtccgattc cggtgtcaca gttccccgac
     7501 gaaggtgctt ttctggtagt agggggggag ccatacggga gcccttcatg caagcaccga
     7561 ccaagaaagt ccttccggag aacgcgtatc gcaaactcga gcccggggaa gtgtacgaac
     7621 ccatcgttcc ccatgacgat aatcgggcgg aagtaaccgt ctggtcgctg tcgctaggcg
     7681 tggtcatggt ggccctgttc gctgccgcgg gaatttatat ggctcttcgc gctggaaacg
     7741 cgatcgagac ggcgattccg attgcgatca tggcggtgtt tttcggcaaa ttgaacaggg
     7801 tgcgatcgac catcctcgag aatgtgatgg tccaatcgat tggtcaagct tctggggtgg
     7861 tggcggcggg cgctgctttc gtcattcccg cgttgtacat caatcaacta cagccttctt
     7921 tttggcatat tttctttgcg accgcgattg gcggctttct cggtgtggtg ctcatcatcc
     7981 cccttcgaaa gtacttcgtg gctgatcaac acggtgagtt gcctttcccg gaagccaccg
     8041 ccaccaatga aatcctt
//
LOCUS       DAOSOR010000234         8049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8932_length_8049_cov_4.459720, whole genome shotgun sequence.
ACCESSION   DAOSOR010000234 DAOSOR010000000
VERSION     DAOSOR010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8932_length_8049_cov_4.459720"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="PK710_13290"
     CDS             complement(<1..781)
                     /locus_tag="PK710_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10745.1"
                     /translation="MPWMLGVLTLVTAVALASCDFKRPPYAVLGHEIGDINCHDGLDN
                     DGDGLIDCEDPDCIYESTWCGEFIPLVEEDYEPEPDLRLVRRYCRMKLPDGADNPYYD
                     IKKCKSAGKAILAVCHDSIDNDFDGQFDCGDKGCSNIQENCCAKEFTNETCSDGIDND
                     QNGFTDCQDFSCRHGLYVTVCVENTDALCSDGIDNDGDGKTDCKDSNCQGTAYCKGKD
                     PENTDAKCSDGIDNDGDGRIDCVDKDCQALDICKPDGRAEDT"
     gene            complement(799..1752)
                     /locus_tag="PK710_13295"
     CDS             complement(799..1752)
                     /locus_tag="PK710_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10746.1"
                     /translation="MVRVSRSVWIWLVCVALSILAVDEASAEPATLVFLNNVPAAVTF
                     NDGDSFRVLEGPNTGSKARLAGFNTLESHGPVHQWGGWHYKELYALAKMATLNARRGV
                     WHCESKDLAADGYGRILWYCLDLAEDQIRHGFAHAMTVSDEPANPRLLAAQHDAIRHR
                     RGMWAKGVPTYVLTSIHSADEGFGKKTYNRLVNTVDGHSKRWYHNAIFSECQDVCHHP
                     TELPMNEAYRVVSELRADAAVAPYVRGIDDILVALSVNTFIQNGFVPKIFGDNAKVQA
                     ALESMKSKGRFASVRSIKGACAIHVDFKRRYVRPKPACLQW"
     gene            2066..3133
                     /locus_tag="PK710_13300"
     CDS             2066..3133
                     /locus_tag="PK710_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10747.1"
                     /translation="MRYRTSVVLVLWLAACGSDDATDDFGGAPDGTSTESSTLDATSD
                     SSPADSHGESDADAHTDGGIDGEPDVGSDVATDVVTDVATDVATDVVADVVADVVADV
                     VADVATDVVTDVATDVVTDVVADVVTDVAEASSGIISVRVGSKNDALASYLMNLSPPA
                     WNIFYGETVPVGRHFDSGHVSYHTAFRFVGVAIPKGAPIASARLRFFPTNEVDSSHAL
                     WLNVYAERTGNSAPFDPSNYTSGRPDQRLKTLSFIDRWLVRCNASCTDMTEYDCPQRK
                     LDCWNRSVAFEVPKDLRSLVQEVVDQADWVSGNALTIFLINSATDADGSKYESSRSIT
                     GYDIARGAEYSPELTITLGSP"
     gene            complement(3139..3825)
                     /locus_tag="PK710_13305"
     CDS             complement(3139..3825)
                     /locus_tag="PK710_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10748.1"
                     /translation="MRSAPRVTVFHEAGQRLTLYDYGTRQELIFGKVPILTSAALGTE
                     RDFGALAKTLCTRSCPRILIGGLGFGATLASVLQVTGQQAEVIVAEKLATVVELVSGP
                     LAHLNPGVLQDPRTQLVQQDVIEVLERERNLDLILLDVDNGPDWASFRSNASLYGDRG
                     LRTAFKALRHGGAYALWSGYPADRFIKRLRRAGFEPLVIPLYERRKLQARAYVGKKPP
                     QKPKSVRPRG"
     gene            complement(3890..5569)
                     /locus_tag="PK710_13310"
     CDS             complement(3890..5569)
                     /locus_tag="PK710_13310"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOT10749.1"
                     /translation="MISFSIDPAVAEQQMHAIIYYLTAFGYVDGDFDRSEKAFIREYI
                     GKLVAKRADDALDPKTASGIRAELVEKWTKHFHEELDRIDQGIQADFLESVAEGEDAR
                     DFVISRLKLRCFELFTGFDDDNRSQLLSLVDELMMADGVIHPNERQFRDELFDLLNAP
                     VDLDPADIIPLESGAVVLEPQVRLAPQQIDHPFFQRSEFDYERNPEVFKKQAEQDLDL
                     IQRTLAKLQQQRDKGKGALGDAVHVDEFKGRDPFLDGHVYVHPSKPGMNCDILVLGDL
                     HGCYSCLKAALLQADFFRKVQAYHDDPVHNPDMKLVLLGDYIDRGRFSYNGVLRTVMQ
                     LFLTVPDHVYMLRGNHEYYVELNGRIYGGVRPAEAINALQDIAPQEMFRAFRSLFEAL
                     PNTLLFDRTLFVHAGIPRDDTLALKWLGLSSLNDPEIRFQMLWSDPSDADVIPTALQQ
                     SSARFPFGRLQFKKFMARLGCSSMIRGHERVTSGFKTIIDDGDVVLLNLFSAGGAQNN
                     DLPPDSTYREITPMALTIRYRDGISQLTPFEIDYARYNDPQHNAFFRNQIR"
     gene            5568..6551
                     /locus_tag="PK710_13315"
     CDS             5568..6551
                     /locus_tag="PK710_13315"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HOT10750.1"
                     /translation="MDGQLNRTSKRIHRLRYLELFCRRSQQKHRDSPMFFPWVRPFLI
                     LFFLLMGTSISTRAHAFCWASTCQPNGVICEHPSSLDCGSPLYWPRECVGFSIQQDGT
                     SYLSFEEASSAIVAGFNVWLEPFCNGQPPGIGLIEMLNVSCDRVEYNSSAGNANVIVF
                     RDKLWTHPESPDKIAVTTITFSPSTGEIYDVDIEINSNQHLFTVSEPTEAYDLVSIMA
                     HEAGHFFGMGHSPEWDATMFEMYEPGSTQQRTLSIDDQRGMCTMYPPWPIDLARCNPI
                     PRHGFSPECRSEQPEHGCTISHSVHHSHAQRWSMLLVALLVFGFSIRRRMH"
     gene            6674..7000
                     /locus_tag="PK710_13320"
     CDS             6674..7000
                     /locus_tag="PK710_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10751.1"
                     /translation="MTTNGHESNGQSFVVGDDGSVTLWLGESCIQTTAKQAYHALMAV
                     LLESDASEADQHAAETLRLFLSEMDFASLRSRYPAMAGGVDCRVRIHLLDERQCLWEI
                     LRNDRG"
     gene            6997..>7353
                     /locus_tag="PK710_13325"
     CDS             6997..>7353
                     /locus_tag="PK710_13325"
                     /inference="COORDINATES: protein motif:HMM:NF025306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4213 domain-containing protein"
                     /protein_id="HOT10752.1"
                     /translation="MNPEQHQSVVHHIVDRLLPTAGDVILADARVGLGYTAVLLQDGR
                     AGLAFTFRQQAVGGCSVFRRRRPLAGSRIADLLALATCTDPLEAAIGLACANALINSP
                     SESTSEGDVLERLEPRS"
     assembly_gap    7354..7363
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7364..7790
                     /locus_tag="PK710_13330"
     CDS             <7364..7790
                     /locus_tag="PK710_13330"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HOT10753.1"
                     /translation="VAMVGWFGPLIEPLRNRTRSLTIYEQDTESHRNSLPAHKAIEGL
                     GHCHIAILTSTCLLNGTADGLLQAARGCREVVLLGASTPLLPEVFQPQGVTMLSGVVI
                     TDADEVLRVVSEAGGTRQFSPYVRKVVVPMSRDQTEKAP"
     gene            7787..>8049
                     /locus_tag="PK710_13335"
     CDS             7787..>8049
                     /locus_tag="PK710_13335"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT10754.1"
                     /translation="MTSSTDEISITLVVDNRAAEGLAVEHGFSAWIEAGDFRILFDTG
                     QGMALPINAHNLGIEPASAHAVVLSHGHYDHTGGLSDILVTSR"
BASE COUNT         1742 a         2268 c         2229 g         1800 t
ORIGIN      
        1 gcgtatcctc ggctcttccg tctggtttac agatatcgag cgcttggcaa tccttgtcca
       61 cacaatcgat tcgcccgtca ccgtcgttgt cgatgccgtc cgaacatttg gcgtcggtgt
      121 tctccgggtc cttgcccttg caatacgctg tcccttgaca attcgaatcc ttgcaatcgg
      181 tcttcccgtc gccatcgttg tcgataccgt cggagcataa agcatcggta ttttccacgc
      241 agaccgtcac gtacaaccca tgtcggcagg agaagtcctg gcagtccgtg aaaccgttct
      301 ggtcgttgtc gatgccatcg gagcacgttt cgttggtgaa ttccttggcg caacaattct
      361 cttggatgtt cgaacacccc ttgtcgccac aatcgaattg accatcgaag tcgttgtcga
      421 tggagtcgtg gcataccgcg agaatggcct tgcccgcgct cttgcacttt ttgatgtcgt
      481 aatagggatt gtccgcacca tcaggcagct tcatgcggca ataacgcctg acaagtcgca
      541 ggtccggttc cggttcataa tcctcctcga ccaaaggaat gaactcacca caccacgtcg
      601 attcatagat gcaatcggga tcctcgcagt cgatgagtcc gtcaccatcg ttgtcgaggc
      661 cgtcatggca gttgatatca ccgatctcgt gaccgagaac ggcataagga ggacgcttga
      721 agtcgcaact ggcgagggca actgccgtca ccagcgtgag aactccgagc atccacggca
      781 tgtaattagg cttgttgttt accactggag acacgctggt ttgggtcgaa cgtatcgacg
      841 tttgaagtct acatgaatgg cacaagcgcc tttgatggag cgaacagaag cgaaacggcc
      901 tttggatttc atgctttcga gcgcggcctg cactttcgcg ttgtcgccaa atattttggg
      961 aacgaacccg ttctgaatga aggtattgac cgaaagagcg acgaggatgt catcgatacc
     1021 gcggacataa ggtgccaccg cggcatcggc gcgcagttcg ctgacgacgc gataagcctc
     1081 gttcatgggc agttccgtcg gatgatggca tacgtcttgg cactcggaaa aaattgcgtt
     1141 gtgataccaa cgcttggaat ggccatcgac cgtattgacg agtcggttgt aggttttttt
     1201 tccgaagccc tcgtcggcgg aatgaatgga ggtaaggacg taggtgggca cgcccttggc
     1261 ccacattcct cgacgatggc gaattgcgtc gtgttgtgcg gcgagcaatc gcggattggc
     1321 cggttcatcg gatacggtca tggcatgggc gaacccatga cggatctgat cttccgccag
     1381 gtcaaggcag taccaaagga tccggccgta gccatccgcc gcgagatcct tcgattcgca
     1441 gtgccagacg ccgcgacgag cgttgagggt ggccatcttc gccaacgcgt atagctcctt
     1501 gtaatgccaa cctccccatt gatgcacggg accgtgactt tcgagcgtgt tgaatccggc
     1561 gagacgcgcc ttggagccgg tgtttggtcc ctccaatacg cgaaaactat caccgtcgtt
     1621 gaacgtaacc gccgcgggta cattattcaa aaacacaagg gtggctggtt ccgccgaagc
     1681 ctcatcgacc gccaatatcg acaaagcgac gcagaccagc cagatccaca ccgatcgaga
     1741 aactcgcacc atgcacctct tctcgccgcg caggtcaatc cgcggatacg gatcagcgcg
     1801 cgaggcacgt gaaagacagt agaaatcgtt aatacctctg gtgttgttga agtcaatcat
     1861 tacaagacgg aaaccgtggt gttccaaacg taaacgaaaa agaaccgtct ttcggctact
     1921 tatccacaca tgggccttgt cgtagcccaa aagtcgaggc acggctccca ggtgcgcaac
     1981 ttccaggctt ctttggttcc cgtggcttcg accggcaaca cttgccacga cttttccata
     2041 tggtacggct acccaagtga tcgctatgcg ttaccgaact tccgtcgttc tcgtcctttg
     2101 gctagcggct tgcggctccg atgatgccac cgatgacttt ggtggggctc cggacggaac
     2161 ctcgaccgaa agctcgacgt tggatgcgac gtccgactca tcgcctgcgg atagccacgg
     2221 ggaaagtgat gcggatgcgc ataccgacgg cggtatcgat ggggaacccg atgtggggtc
     2281 agacgttgcg acggatgtag tgacggacgt tgcgacggat gtggcgacgg atgtagtggc
     2341 ggatgtagtg gcggatgtag tggcggatgt agtggcggat gtggcgacgg atgtagtgac
     2401 ggatgtggcg acggatgtag tgacggatgt agtggcggat gtggtgaccg atgtggcgga
     2461 agcaagcagt ggcatcatat ccgtacgggt ggggagcaag aacgatgcgc tggcttccta
     2521 tctcatgaac ctgtctcctc cggcctggaa cattttttac ggtgagacgg tgccggtggg
     2581 acggcacttc gactcgggcc atgtctcgta tcacaccgcg tttcggtttg tgggtgtggc
     2641 gattcccaaa ggcgctccca tcgcttcggc tcgccttcgc tttttcccga ccaacgaagt
     2701 ggattcgtcg catgccttgt ggctcaacgt ctacgcggaa cggacgggca attccgcgcc
     2761 cttcgatccg tcgaattaca cgagtggcag acccgatcaa cggttgaaaa cactatcctt
     2821 catcgatcgt tggttggttc gatgcaatgc ttcttgcacc gatatgactg aatatgattg
     2881 ccctcagcga aaacttgatt gttggaatcg aagcgtggcg ttcgaggtac cgaaagacct
     2941 tcggtccctg gtgcaagaag tggtggatca ggccgattgg gtttccggca atgcactgac
     3001 gattttcctc atcaactccg caacggatgc agatggttcc aagtacgaga gttcacggtc
     3061 catcacgggt tacgatatcg ctcgcggcgc agagtactcc cctgagctga ccatcacgct
     3121 tggcagtccg tgacagggtc atccacgggg gcgcacggat ttaggttttt gcggtggctt
     3181 ttttccgaca taggcacgcg cttggagctt ccgtcgttca tacagcggga ttacaagcgg
     3241 ctcgaatccg gcgcgtcgta gccgtttgat gaaacggtcc gcgggatatc cgctccataa
     3301 ggcgtaggca cctccgtgtc gcaaggcctt gaaagccgtg cgcaatcctc ggtctccgta
     3361 caggcttgca ttggaacgaa aggaagccca atcaggaccg ttgtccacat cgagcaaaat
     3421 gagatccaaa ttgcgctcgc gctcgagcac ctcgatgaca tcctgttgca cgagctgggt
     3481 gcgagggtcc tgcaacacac ccggattcaa gtgggcaagc ggtccggaca ccagctctac
     3541 gactgtcgcc agtttttcag ctacgatgac ttccgcctgt tgcccggtga cttgcaacac
     3601 gctggcgaga gtggctccaa atccgagacc accgatgagg atacgagggc aagagcgcgt
     3661 gcaaagcgtt ttcgccaacg cgccgaaatc ccgttccgtg cccagcgccg cactcgtcaa
     3721 aatcggcact tttccaaata tcaattcttg acgcgtgccg taatcataga gcgtcaaccg
     3781 ctggccggct tcgtgaaata ccgttacccg aggagcagat cgcacggtca gaaactagag
     3841 catacgatgg tcagaagcca gcacggcagc ggctcctgcg ccgcggtggt catcggatct
     3901 ggttgcgaaa aaaggcgttg tgctgtgggt cgttgtaccg ggcataatcg atctcgaagg
     3961 gcgtgagctg tgaaattccg tcgcgatatc gaatggttag cgccattggg gtaatctccc
     4021 ggtaggtgct atccggggga agatcgttgt tttgggctcc ccccgcggaa aaaaggttga
     4081 gcagcaccac atcgccatca tcgatgatgg ttttgaaccc cgacgtcacc cgctcatgac
     4141 cacgaatcat gctcgagcag cccagccgcg ccatgaattt tttgaattgc aaccgtccaa
     4201 aagggaaacg agcactggat tgctgtagtg cggtaggaat gacgtccgca tcgctaggat
     4261 cgctccacag catttgaaaa cggatctccg gatcgttgag ggaactgagt ccgagccact
     4321 tgagggccag cgtgtcatcg cggggaatgc cggcatgcac gaataaggtg cggtcaaaga
     4381 gtagcgtatt gggaagcgct tcgaacaagc tgcgaaaggc gcgaaacatt tcctgaggcg
     4441 caatgtcttg caaggcgttg atggcctcgg ctggacgcac gccgccgtag atccgcccgt
     4501 tgagttccac gtaatactcg tggttgcccc gcaacatata gacgtggtct ggcacggtca
     4561 ggaaaagctg catcaccgta cgcaacaccc cgttgtaact gaagcggccg cgatcgatgt
     4621 agtcaccgag aagcaccagc ttcatatctg ggttatgcac ggggtcgtcg tggtaggcct
     4681 ggacttttcg gaaaaagtcc gcctgcaaca gcgcggcctt caaacatgaa taacacccgt
     4741 gaagatcccc cagcaccagg atgtcacagt tcatgcctgg cttcgaagga tgcacataga
     4801 catggccgtc gagaaaagga tcccgccctt tgaattcatc gacatggacc gcatccccga
     4861 gggctccttt gcccttgtct cgctgctgtt ggagcttcgc cagggtgcgt tggatgagat
     4921 cgaggtcctg ttcggcttgt ttcttgaaca cctccgggtt gcgttcgtag tcgaattccg
     4981 aacgctgaaa gaacggatga tcgatctgtt ggggagccag acgaacctgg ggttcgagca
     5041 ccaccgcacc cgactcgagg ggaatgatat ccgctggatc caggtcgacg ggcgcgttca
     5101 acaggtcgaa tagctcatcc cgaaactgtc gctcgttggg atgaatgacc ccatcggcca
     5161 tcatcaattc atccaccaag gacaacaatt gggagcggtt atcgtcgtcg aaaccggtga
     5221 ataattcgaa acatcgcagc ttgagccgtg atatcacgaa gtctcgggca tcctcgcctt
     5281 ccgccacgga ttccaaaaaa tccgcctgaa tgccttggtc gatccggtcg agttcttcat
     5341 gaaaatgctt ggtccacttt tccacaagtt ccgcgcggat acccgaagcc gtcttcggat
     5401 ccaaagcatc gtcggctcgc ttcgccacga gcttgccaat atactcacga atgaaagctt
     5461 tttcgctgcg gtcgaagtcg ccatccacat agccgaaagc ggtcaagtag tagatgatgg
     5521 cgtgcatctg ctgctcagct acggcaggat cgatcgagaa actgatcatg gacggccagc
     5581 ttaacagaac ctcgaaacga attcatcgtc tgcggtatct cgaattattt tgccgaagga
     5641 gtcagcaaaa acaccgcgat tcccctatgt ttttcccatg ggttcgcccg tttctcattc
     5701 tctttttcct gctgatgggg acttcgatct cgacacgagc gcacgcgttt tgctgggcga
     5761 gcacgtgcca acccaatggc gtcatctgtg agcacccctc ttccctcgat tgcggctcgc
     5821 ccctttattg gcccagagaa tgtgtcggtt tcagcatcca acaagatgga acctcctact
     5881 tgtcgttcga agaggcatcc tcggccatcg tggctggctt caacgtctgg ctcgagcctt
     5941 tttgcaacgg ccaaccacca ggcatcggcc tcatcgaaat gctgaacgtg tcttgcgatc
     6001 gcgtcgaata caactcctcg gcaggtaacg cgaatgtcat cgtgttccgc gacaagcttt
     6061 ggacccaccc cgaatccccg gacaaaatcg cggtcaccac gataaccttc agccccagca
     6121 ccggagaaat ctacgatgtc gatatcgaaa tcaacagcaa ccaacacctg tttaccgtga
     6181 gcgaacccac ggaagcctac gatctcgttt ccatcatggc ccacgaagcc ggccactttt
     6241 ttggcatggg tcactctccc gaatgggatg ccaccatgtt cgaaatgtac gaaccagggt
     6301 cgacacaaca acgtaccctg agcatcgacg atcaacgagg gatgtgtacg atgtaccctc
     6361 cgtggcccat cgatctcgct cgctgcaatc caatccctcg ccacggattc tcccccgagt
     6421 gtcggtccga gcagccagaa cacggttgca ccatcagcca ttcggtgcat cattcccacg
     6481 cgcaacgatg gtcgatgctc cttgtcgctc tgctcgtctt cggtttttcc atccgtcgcc
     6541 gcatgcattg aatatctccc ccaactcgct tccccctcgt tgtcgcgttg caccattgct
     6601 acgttcgggc atatttctct tcggcatttc gcatcgaatc ccatcgtcgt gatgcaagat
     6661 cgccccatcc tcgatgacaa cgaatggtca cgaaagtaac ggacagtcgt tcgtggttgg
     6721 cgatgatgga agcgtcacac tatggcttgg agaaagctgc attcaaacga ccgcgaaaca
     6781 agcctatcac gcactcatgg ccgtcttgct cgagtccgac gcctccgaag ccgaccagca
     6841 cgcggcggaa acattgagac tatttctttc ggaaatggat ttcgcttcgt tgcgatcccg
     6901 atacccggcc atggcgggag gcgtggactg tcgcgtgcgg atccacctgc tcgatgaaag
     6961 gcaatgccta tgggaaatcc tgagaaacga tcgcggatga accccgagca acaccaatcc
     7021 gtcgttcatc acatcgtgga tagactcctt cccacggcgg gtgatgtcat cctggcggat
     7081 gctcgtgtcg gccttggcta taccgcggtg ctgctgcagg acggaagagc gggattggcg
     7141 tttacgttcc gacagcaggc cgtcggtggg tgctcggtgt ttcgtcggcg acggcccctt
     7201 gctggctcgc ggattgccga ccttcttgct cttgccacct gcaccgaccc cttggaagcc
     7261 gcgattggat tggcttgcgc caatgcgctc atcaattctc cgagcgagtc gacatcagaa
     7321 ggcgatgtgc tggaacgact cgagccgaga tctnnnnnnn nnncgtggcg atggtggggt
     7381 ggtttggacc gttgatcgaa ccgcttcgaa atagaacacg gtcgttgacc atctacgaac
     7441 aagacaccga atcccatcgt aattccttgc ccgcgcacaa ggccatcgaa ggactcggtc
     7501 attgtcacat cgcgatcctg acttccacgt gtttgctcaa cggaaccgca gacggtttgt
     7561 tgcaagccgc cagagggtgt cgtgaggtgg tcttgctcgg tgcctccaca cccctgcttc
     7621 cagaggtttt tcaaccacaa ggagtcacga tgctatccgg tgtggtgatc accgatgccg
     7681 atgaagtgct tcgcgtcgtt tccgaagcag gaggaacacg ccagttttct ccgtatgtga
     7741 gaaaagtggt cgttcccatg tctcgcgatc agacggaaaa ggcaccatga catcgtccac
     7801 cgatgaaatc tcgatcacgt tggtcgtcga caaccgagcc gcagaaggct tggcggtaga
     7861 acatggcttc agcgcgtgga ttgaagcggg cgactttcgc atcctattcg ataccgggca
     7921 agggatggcg cttcccatca atgcccataa ccttggaatc gaaccggcct ccgcccacgc
     7981 ggtggtgctg agccatgggc attacgacca taccggcggt ctttccgata ttcttgtaac
     8041 atcgcggaa
//
LOCUS       DAOSOR010000235         8006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_8995_length_8006_cov_4.840901, whole genome shotgun sequence.
ACCESSION   DAOSOR010000235 DAOSOR010000000
VERSION     DAOSOR010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 8006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8006
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8995_length_8006_cov_4.840901"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1918
                     /locus_tag="PK710_13340"
     CDS             <1..1918
                     /locus_tag="PK710_13340"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT10755.1"
                     /translation="ISCYSSYDYLKTMLMGETLRRFVPRARLVVGGYHPSARPEDFTG
                     NDSPFDYVVIGDGERPLRGLVDALIRGKPPTQRVLPVEPVQRSDRIPDLDWSLLDRYR
                     PVARKVASQAQLFLSRGCPYDCSFCMERAKRCTAWRAYEPERAVEELHRLHAFLDLSS
                     WTLFVTDALFGCSKSWRRVFFEQFVRKPIPVRKIWLLTRADLLEREDLEWMRRANIAP
                     GFGIESGDPSQLARIQKAGSPQQFLDHFRQVANWARELDLPFGANVIVGHPGETEETL
                     RASHAYLESVFLSGDRTTGFLSIDPFRFYPGSGIDQNLESWKRLTGMTVHRPAWWHDG
                     DQDYLSEWVDPSFELDFRKTLRLRFELFGPLLRKIASHFSVRDPEEAYFFRSVEDAVD
                     QHEPYRHLRAIGLWHLWNELVGYESDTRKRVVDDEELAQVAREARRQTLRDRRFDGIA
                     PALHEALVWVPRERFVRVEDIDRSAQDRAFLLNESGEATLSAMHAYAMSFQALALKEG
                     DTLVDLGGGTGYGAAIAAHVVGIHGAVLTMDVDEGLSALAANILAQYGNIRVECANAH
                     DVSRWKGATKVSVGFAVKKIPDLWIESLDPGGKMVVPVGEARSQQLMLVERKPGGVKM
                     TSLGPVRYVLDRGD"
     gene            complement(2129..2473)
                     /locus_tag="PK710_13345"
     CDS             complement(2129..2473)
                     /locus_tag="PK710_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10756.1"
                     /translation="MVHADGVENDSNASCGGIRVKDRLSRKLDGFLSKFSRECFNAET
                     LVTDAENSNYRRAMVDKLAATLNNPADNEEFVKWLIASIYKGRRLNTVLARLKDLLRQ
                     AMEATRMRSRLP"
     gene            2756..4768
                     /gene="tkt"
                     /locus_tag="PK710_13350"
     CDS             2756..4768
                     /gene="tkt"
                     /locus_tag="PK710_13350"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOT10757.1"
                     /translation="MQTIDQLCVNTLRIMAADMAQRANSGHPGFPMGAAPMGYVLWQR
                     HLLIDPSAPTWPDRDRFILSPGHGSPLQYSLMHIAGFEDVPIEALKAFRQWGSKTPGH
                     PEFGLTAGVEATTGPLGQGAANAVGMAIAERMLAHRFNRPGHEIVDHHTYAIVSDGDM
                     MEGVSAEAASLAGQLGLGKLICLYDANDVTLDGPTSLTFSVEDVGKRYEAYGWHVVFV
                     ADGNHDLEGLDRALSEARAETQRPSMIIVKTTIGYGSPNKQGSNASHGAPLGVEELAL
                     TKKNLGWDPSWHFHVPEAVRAHIGKGMQRGHEARVAWENRFRAYEKAYPELAEQWKLA
                     WEGALPDDWDVDLPSFSPGEDQPTRNASGKALNAIASRVPWLVGGDADIGSSTQSQIK
                     QGGSFDGVTGAGRNIHFGVREHAMTAIANGMAYHGAVRPLVSTFFTFVDYMRPSIRIA
                     ALAHLPILMVFSHDSLAVGEDGPTHQPVEQLASLRLMPNVIVLRPADPNETTEAWRWC
                     MQYSDGPVALVTTRQKVPVIDRAKYAPACGLHRGAYILTDANAPADVVVIATGSEVSL
                     ALQAHEQLASEGIAVRVVSMPSWEIFQSQPSSYIEQVLPSDIPRVAVEAGVSFGWERW
                     VKHRRALVTVDQYGSSAPGHVAMEKYGFHVHRVVAAIRQVIAEKKS"
     gene            4773..>8006
                     /locus_tag="PK710_13355"
     CDS             4773..>8006
                     /locus_tag="PK710_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10758.1"
                     /translation="MDLAAHELALRHAAGAVVVDPRFLRRVIKYHHRLSGLVPHDQCY
                     PIAKTELLALAGAEELGLPATDIPDPVILIARPSPRFLRTRSDTEVITRLWRDVFHAR
                     VHLALARRQADGCLDDVQIRKRIELIGPTEFNEIRAILSHDDLVLPPGTDREVYVEFV
                     AVYLELRHFAPRLLVTTFPGLSAFDKVDEAIALDVDPLPLLSKGRPVAVQQALGPTGT
                     VGPWSPISVRQPLVAPSKEIPSKAYRRLEGRARKAQEVGNDVRAVLHWAVACGALEPQ
                     LRQQAEESLQQCCGHLGQRLHAALGSSIDRADDIDWPSLIVVLAHRAAADSSRIRYGI
                     EARVLYTLQRASLAAEKQHEAVDVASYILSRGKRKIVRKLESTKELRVARAFQVALAR
                     AQHVRLTSSERKRLTDALGHAVRIADRRTREALRPRFRRVFDQVGLVATSTPEQLSRD
                     KLIEELMDQVLLSGYLTFPQLRDAISRNQLKLADLKGSSEWIHGDALLEADRRLETQL
                     DGIYRRSDIYLRGMQKASSLSFGTRVGRVISLYVLLPLGAAFVLLEGVGHLVGPLWRL
                     FGAAAPNVLTLTSFFVTSTILFGILHSEPLRAFGMQILEMLGVVIAWVFFRIPRAILT
                     IPAVQQWFARPGVRLVLRRIVVPILLAVVAFYLTPLRKEDWFLGLAGSLATFVTVSLL
                     AGTRIGQRVEDYVLEQLVPTWQVLSHQWIPQLLQLIARSFAVAMEWLLRGMIRVDELL
                     RYQQGHHGLWTVVRASFGLCWAMVAYVIRVYVTLLVEPELNPLKHFPIITVAHKLTLP
                     FAPQMLAALQIPLSPLGPIVGGAIAGVTAFLAPSVTGFLAWELKENYKLYRATIPDRL
                     VPARFGAHGETMRGLLVAGIHSGTLPKLYERLRRAASREHEQVASQIARVADGKSIEG
                     SGAAKFREGIAEVERAIRRFVERELLAYLLKSSRWRFGPVSIASVELSSNRIRIRLTC
                     EQLGRDACEIAFEQQGGRILAGMPSVGFVDVLVRRSRTSARIFENALAGFYQRAEVDL
                     VREQIEEELGEGTHFEIAEEGLVCWPGNDYRTEYAYGVDAKRPKALAP"
BASE COUNT         1591 a         2129 c         2435 g         1851 t
ORIGIN      
        1 gatatcctgt tattcctcgt acgattattt gaaaacgatg ctgatgggcg agactttgcg
       61 gcgctttgtc ccgcgcgctc gccttgtcgt gggggggtat catcccagtg cgcgacccga
      121 agattttacg ggaaatgact ctcctttcga ttatgtcgtg attggcgacg gcgaacgtcc
      181 gttacgcggt ctggtcgatg cacttatccg ggggaaaccg ccgacccagc gggtgttgcc
      241 ggtcgagccg gtccagcgat ccgacagaat tccggacctg gattggagct tgctcgatag
      301 gtatcgtccg gtagcgcgaa aggtagcgtc tcaagcgcag ctatttcttt cccgaggctg
      361 tccttacgac tgctcgtttt gcatggagcg ggcgaaacga tgcacggcat ggcgcgcgta
      421 cgagcccgaa agggcagtag aggaattgca tcgcttgcat gcttttctgg atctttcgtc
      481 gtggacgctt tttgtgaccg atgcgctctt tggatgttcg aaatcgtggc gccgcgtgtt
      541 tttcgaacaa ttcgttcgaa aaccgattcc cgttcgcaag atttggctgc tcacccgcgc
      601 tgatcttctg gaacgggagg acctcgagtg gatgcgtcgt gcgaatatcg ctccaggctt
      661 tggtatcgag tccggagacc cttcgcagtt ggcacgaatt cagaaggccg gttctccgca
      721 gcaatttttg gatcattttc ggcaggtggc gaactgggcc cgcgagcttg acttgccttt
      781 tggcgccaac gtcatcgtcg ggcacccagg agaaaccgag gagactctgc gagcttctca
      841 tgcctatctc gaaagcgtct ttctcagtgg cgatcgaacg actgggtttt tatccattga
      901 cccatttcgt ttctacccgg gctccgggat cgatcagaat ctcgaatcgt ggaagcgtct
      961 tacgggaatg acggtgcatc gtccggcatg gtggcacgat ggtgaccaag attatttatc
     1021 cgaatgggta gacccttcgt tcgaactcga cttccgaaaa acgcttcgct tgcgctttga
     1081 attatttggt cctctgttac gaaagatcgc ttcgcacttt tccgtaagag accccgaaga
     1141 agcgtacttt tttcgatccg tggaagatgc cgtagatcag catgagcctt accggcattt
     1201 gcgagcaatt ggtttatggc atttatggaa tgaattggtc ggatacgaat cggatacgag
     1261 aaagcgggtc gtggatgatg aggagcttgc ccaggtagcc agagaggcgc gtcggcaaac
     1321 acttcgggac cgaagattcg atggtatcgc ccccgcattg catgaggctc tggtgtgggt
     1381 gccgagagaa agattcgtac gggtggaaga catcgatcga tccgcgcaag atcgtgcgtt
     1441 tcttctgaac gaatccggtg aagccacctt gtcggccatg catgcctatg cgatgtcgtt
     1501 tcaggccctt gccttgaaag aaggagacac gctcgtcgat cttgggggag gtacaggcta
     1561 cggagccgcg atcgcggctc atgtcgtggg aatacatggg gcggtgttga cgatggacgt
     1621 ggacgaaggg ttgtcagctt tggctgcgaa tatccttgcg caatacggta atattcgcgt
     1681 ggaatgcgcc aacgctcacg acgtatcgcg ctggaaaggg gctaccaagg ttagtgtggg
     1741 gtttgcggtg aaaaaaattc cagacctttg gatcgaatcc cttgatcctg gcggcaagat
     1801 ggttgttccg gtaggagaag cccgcagcca gcaactcatg ttggtggaac gcaagccggg
     1861 tggtgtgaaa atgacaagct tgggaccggt gcgttacgtg ttggatcggg gagattaagg
     1921 gaggctggcc gcggagggca gcgggttatg cgccgcgggc aaccgtagcg acgcattgcg
     1981 ttgtcttgaa atccccttcg cgccacggtt ggcggctggt ttcgcgccgg gtgcgtgatc
     2041 gcgctgacgg cttatgtcgt tgaggatcga gttggttttc tacaggcgtt cggatttcga
     2101 aaaggcctgt aggctcgagt ttgctccatc agggtagcct actccgcatc cgcgtggcct
     2161 ccatcgcctg tctgagcaga tccttgagcc ttgccagcac cgtgttgagc cgtcttccct
     2221 tgtagatgct ggcgatcagc cacttgacga actcctcatt gtcggccgga ttgttcaagg
     2281 tggccgcgag cttgtccacc atcgctcggc ggtagttgct gttctcggcg tcggtgacca
     2341 aggtctcagc gttgaagcac tcgcggctga acttggagag gaagccatcg agctttcgag
     2401 acaagcgatc tttgacccgt atcccaccgc aggaagcatt cgaatcgttc tcgacgccat
     2461 cagcgtgaac catcaagcgc cgtgatccgt acgcgcggca gtacttccgc ttgcctcttc
     2521 tagattgatg taacctattg attaataacg ataaatatcg gtatcaacaa gagccttccc
     2581 tcgcgctccg cgcaccgacg agcaccacgt gttgacctgg tccctgccat ggtgtcacag
     2641 cgtttttggc aagtgattcg ctgaaaccac acaacttcct tccccagtgg ccacccgcgc
     2701 cctttcgggt taaggaagcg tcgtcgatga ccaaggagac gggatgcacg gggaaatgca
     2761 aacgattgat cagctatgcg tgaacaccct tcgaatcatg gccgcggaca tggcgcaacg
     2821 agccaattcg ggacaccccg gttttccgat gggtgcagct ccgatgggct acgtgctttg
     2881 gcaacgtcat ctgctcattg atccatccgc gccgacgtgg cccgatcgcg atcgattcat
     2941 tttgtctcca ggccatggaa gccctttgca atacagtttg atgcatatcg cgggattcga
     3001 ggatgtaccc atcgaagcgt tgaaagcttt ccgacaatgg ggcagcaaga caccggggca
     3061 tcctgagttt ggactcaccg cgggcgtgga agccaccacg ggaccgttgg gtcagggggc
     3121 tgccaatgca gtgggaatgg cgatcgccga gcgcatgctc gcgcaccgat tcaatcgtcc
     3181 gggtcacgag atcgtggacc atcacaccta cgccatcgta tccgacggcg acatgatgga
     3241 aggcgtatcg gcggaagccg cgtctctcgc ggggcaattg gggttgggta agctgatatg
     3301 cttgtacgac gccaacgatg tcacgttgga tgggcccacg tcgttgacct tttccgtgga
     3361 agacgtggga aaacgctatg aagcgtatgg gtggcatgtc gtgttcgtgg cggatgggaa
     3421 ccacgatctg gaaggtcttg atcgtgcgct atcggaagcc cgtgccgaaa cacaaagacc
     3481 gtcgatgatc atcgtgaaga cgacgattgg atatggttca ccgaataaac aagggtcgaa
     3541 cgcatcgcat ggggcacctc tcggtgtgga agaactcgcg ctgacgaaga aaaacctggg
     3601 ttgggatccg tcgtggcact ttcatgtgcc ggaggccgtt cgcgcgcata ttggcaaagg
     3661 gatgcagcgc ggccatgagg cccgagtcgc ctgggaaaat cggtttcgcg cgtatgaaaa
     3721 ggcgtacccg gagctggcgg aacaatggaa actggcatgg gaaggagcgt tgccggatga
     3781 ttgggatgtg gacttgccga gcttttctcc cggtgaggat caacccacga gaaatgcctc
     3841 tggcaaagcg ctcaacgcca tagcgtcgcg cgtgccatgg ctggtcggag gcgatgcgga
     3901 tatcggcagt tcgacacaaa gccaaatcaa acaaggtggc tcgttcgatg gcgtgacagg
     3961 cgcggggcgc aacattcatt ttggggtacg cgaacacgcc atgactgcca ttgctaatgg
     4021 catggcttat cacggtgccg ttcggcccct cgtatccacg ttctttacgt tcgtggatta
     4081 tatgcggcct tccattcgta tcgcagcgtt ggcgcatctt cccatcctca tggttttttc
     4141 gcatgactcg ctcgcggtag gcgaagacgg tcccacccac caacctgtcg aacaactcgc
     4201 atcccttcgc ctcatgccga atgtcatcgt tttgcgaccg gccgatccga atgaaaccac
     4261 cgaagcgtgg cgttggtgta tgcagtattc cgatggtccc gtagcgctcg tgacgacccg
     4321 ccagaaggtt ccggtcatcg accgcgccaa gtatgcaccg gcttgcgggc ttcatcgagg
     4381 agcttacatc ctgacggatg caaacgcgcc cgcggacgtt gtcgtgattg ccaccggttc
     4441 ggaggtttcc cttgccttac aagcacacga acagcttgct tccgaaggca ttgccgttcg
     4501 cgtggtgtcg atgccaagct gggaaatttt tcaatcgcaa ccgtcgagct atatcgagca
     4561 ggtgcttcct tcggatatcc cccgcgtggc cgtcgaggct ggagtcagtt tcggctggga
     4621 aaggtgggtg aaacatagac gtgcgcttgt gaccgtggac caatatggaa gctcggcccc
     4681 tggacacgta gcgatggaaa aatacggatt tcatgttcat cgagtggtgg ccgcgattcg
     4741 ccaggtgatc gcggaaaaga agtcgtaatc gcatggatct tgccgcccac gaactcgctt
     4801 tgcgtcatgc cgctggagcg gtcgtggtgg accctcgctt tcttcgtcgc gtcatcaaat
     4861 accatcacag gctctcggga ttggttcccc acgaccagtg ttatccaatc gcgaaaaccg
     4921 aattgctcgc gctcgcgggc gcggaagagt tgggattgcc agcgaccgac attcctgatc
     4981 ccgtcattct catcgcgcgt ccgagtcctc gtttccttcg cactcgttcc gacacggagg
     5041 tcatcacgag actgtggcgt gatgtatttc acgcgcgtgt gcatcttgcg ttggctcgca
     5101 gacaggcgga tggttgcctc gacgatgtcc agatccggaa gcgtatcgag ctgatagggc
     5161 cgacggaatt caatgaaatt cgcgcgattt tgagtcatga cgacctggtt cttccgccgg
     5221 gcacggaccg tgaagtgtat gtagagttcg ttgcggttta cctggaactc cggcacttcg
     5281 ctcctcgact tctggtcacg acatttcccg gattgagtgc gtttgacaaa gtggatgaag
     5341 ccatcgcgct cgatgtcgat cctttgcctt tgctatcgaa agggcgcccg gtcgcggttc
     5401 aacaagccct gggaccgacg ggaacggtgg ggccttggag tcccatctcc gttcgacaac
     5461 cgctggttgc cccatcgaaa gaaatccctt ccaaggctta tcgaaggctc gagggccgcg
     5521 cgcggaaagc acaagaggtt ggcaatgacg tgcgggccgt gctgcattgg gcggtggcgt
     5581 gtggagcact cgaaccacaa ctacgacaac aagccgaaga gtcgttgcag caatgttgtg
     5641 ggcatctggg ccagcgcttg catgcggcgc tgggctcttc catcgaccgg gccgatgata
     5701 tcgattggcc gtcgcttatc gttgttcttg ctcatcgtgc cgccgcggat tcgagtcgga
     5761 ttcggtacgg gatcgaggca agagtgttgt acacgttgca acgtgcatcg ctcgcagccg
     5821 aaaagcagca cgaggctgtc gacgtggcgt cgtatatcct ttcgcgcgga aagcgaaaga
     5881 ttgtaagaaa gctggagtcc acgaaggagc tgcgagtggc tcgagccttt caagttgcgt
     5941 tggctcgggc acaacacgtg cggctcactt cttccgagcg aaaacgactc acggacgctc
     6001 tcggtcatgc ggttcgaatc gctgatcgaa ggactcggga ggcgcttcgt ccacgttttc
     6061 gacgtgtgtt cgaccaggtg gggctcgtgg cgacgagtac cccggaacaa ttgtcccgcg
     6121 acaaactcat cgaggagttg atggatcaag tcctgctcag tggatatctg acttttccgc
     6181 aacttcgcga tgccatctcc cgcaatcaac tcaaactagc tgatctgaag ggttcctccg
     6241 aatggatcca tggcgacgcg ttgctcgaag ccgaccggcg gctcgaaacg caactcgatg
     6301 gcatctatcg tcgaagtgac atttacttac gaggaatgca aaaggcgagc agcctgtcgt
     6361 ttgggactcg cgtgggaaga gtcatctcgt tgtatgtgct gcttccgttg ggcgcggcgt
     6421 tcgtgctgct cgaaggcgtg gggcatctgg tggggccgct gtggagactc tttggcgccg
     6481 ctgcgccaaa cgtgttgacc ctcacctcgt tttttgtaac atcgactatc cttttcggga
     6541 tacttcatag cgaaccgctt cgggcctttg ggatgcaaat cctggagatg ctcggcgttg
     6601 tcatcgcttg ggtgttcttt cgcattccta gggccatcct caccatcccc gcagtgcagc
     6661 aatggttcgc tcgcccaggc gtacgactcg tcctgcgtcg gattgtcgtg ccgattcttt
     6721 tggctgtcgt tgcgttttat ctcacaccct tgcgaaaaga agactggttt ctcgggctgg
     6781 cgggttcgct ggcgaccttt gtcaccgtga gtctgctggc gggtacgcga atcggccagc
     6841 gagtcgaaga ttacgtgctc gaacaactcg tgcccacatg gcaagtgctg agtcaccagt
     6901 ggattccgca actgcttcag ctcattgcaa gatcgtttgc cgtcgcgatg gagtggttgc
     6961 tacgaggaat gattcgcgtg gatgaactgc tgcgctatca gcaggggcac catggtctgt
     7021 ggacggtcgt gagggcttcg ttcgggttgt gttgggccat ggtggcgtac gtgattcgcg
     7081 tgtatgtcac gctgctggtg gagccggaac tcaatccgct gaagcatttt ccgatcatca
     7141 ccgtggctca taagctcacg cttccttttg ctccgcaaat gcttgccgcg ctgcaaattc
     7201 cgctgtcgcc gctgggcccc atcgtcggag gggccatcgc gggcgtgacg gcgtttctcg
     7261 cgccaagtgt cacgggcttt ctagcgtggg aactgaaaga gaattacaaa ctgtatcgag
     7321 ctacgatccc tgaccgtctg gtgcccgcgc ggtttggcgc tcatggggaa acgatgagag
     7381 gccttctcgt ggcgggaatt cactcgggaa cactgcctaa actgtatgaa cgattgcgac
     7441 gcgccgcctc tcgagaacac gaacaagtgg cctcgcaaat cgcgagagtg gccgatggca
     7501 aatcgatcga gggcagtggt gcggccaagt ttcgcgaagg gattgccgag gtggaacgag
     7561 ccattcgacg cttcgtggag cgagagctgt tggcttatct tttgaaatcc tcacgctggc
     7621 gcttcggtcc ggtttctatc gcaagcgtgg aattgagttc caaccgtatt cgtatccgat
     7681 tgacgtgcga gcagcttggt cgcgatgctt gcgagatcgc tttcgaacag cagggaggac
     7741 gaattctggc agggatgccg tccgtaggat tcgtcgacgt gctcgtgcgg cgctcacgaa
     7801 cgtcggcgcg catcttcgaa aacgcactcg cagggtttta tcagcgcgcg gaagtcgatc
     7861 tggtgcgcga gcaaatcgaa gaagaattgg gcgagggaac gcatttcgag atcgccgagg
     7921 aaggcctcgt atgctggccc ggaaacgatt atcgaacgga gtatgcctac ggcgtggatg
     7981 ccaagcgccc caaagccctg gctccg
//
LOCUS       DAOSOR010000236         7999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9009_length_7999_cov_4.529204, whole genome shotgun sequence.
ACCESSION   DAOSOR010000236 DAOSOR010000000
VERSION     DAOSOR010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7999
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9009_length_7999_cov_4.529204"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2377
                     /locus_tag="PK710_13360"
     CDS             <1..2377
                     /locus_tag="PK710_13360"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOT10759.1"
                     /translation="YLFDLNYIRSSLEDIRAAVGQIIEQLVLLSDGRCRPLRQRFEQI
                     DGQIARMFPDYRKLEPTAYVLWFDEIGREHALSVGSKNAQLGEMRSRLGMTVPNGFAV
                     TAWAYKRFFDANGLSDRIRSLVQNADVCSFASLVEVSEKVRAMVMEARVPADLSEAIV
                     QAFQRLVAGSSGKRVAVRSSGLEEDSLFSYAGQYATLLNVGASEAVSAYRQVVASKYT
                     PQAIYHYLSHALGEAELAMSVGYVQMVDARASGVVYTRDPVYPDTDSLWIHAVLGLGA
                     YLVDGTLTPDAFNYSRSDLVEQERQIVEKKVRLVLDSSGGTVQERVPDEEQTRPSVSE
                     KELRELAQLGLRLEEHYGVPQDIEWAIDAQGSLQLLQTRPLQLVKRSTTPRTFEVDGK
                     DVLLEGATTASPGAGAGPVYQVRTSVDLLGVNDGMVVIAPNPFPGLITIMGKARALVT
                     EVGGVASHMATLSREYRLPTLVGASGAMGIPNGTSVTVDASGGRILAGLHFDLAEARV
                     AETNNLFEDTPLFQGFRRILDLVAPLHMLHPSDPSFLPENCRTLHDITRYAHQKSMEE
                     MFRRVSTLETTAEFGQPVVSELPLPVRVLYVDRPMTDLGEGKGVTMQEIDSEPMLAIW
                     SGMREQGWPSGPSSTRTPFPTRLKTRGKRSEPFQYTVKSYAVLGTHYMMLCLHLGYHF
                     TTIEALCTEDANKNYVRLQYKDGGASLERRVRRIRLVATLLSRLGFENQTEADFLDAL
                     YSHADCETVQDKLRLLGRITMLTKQLDMALSSDAITEWYTKDFLSKLGLES"
     gene            2523..3074
                     /locus_tag="PK710_13365"
     CDS             2523..3074
                     /locus_tag="PK710_13365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT10760.1"
                     /translation="MPQHILAVDDEPHMLKLLARIVEDQTPYQMTTTSNSLEVPALLE
                     AQSFDVVVTDLRMPGLDGFDIVRMVNERGLGAHVIMITAFGGMEAAMKALSEGVFEYI
                     HKPFRKEAFLFSLHRAMQSAKTRREALRLEEMIAMEPYGEARRAFDREYVRRLQEREP
                     NDLSAQAERSGLDPNWIDGLRHG"
     gene            3123..3635
                     /locus_tag="PK710_13370"
     CDS             3123..3635
                     /locus_tag="PK710_13370"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOT10761.1"
                     /translation="MKPKTAGELMIPLDQYPHLPYWFTLRQAIAEMEKSEFNVDGRKS
                     LPRVVLVFDERYQLLGLVRRRDILRGLEPPFLTRPADDDFAALRDPNLLELSYDNMID
                     GIRDRAERPVRDVMRPFRATADHEDPLMKLIHTMVEHNLHLLPVLKEGSVAGVVRTVD
                     VFREVARLVL"
     gene            3664..5190
                     /locus_tag="PK710_13375"
     CDS             3664..5190
                     /locus_tag="PK710_13375"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOT10762.1"
                     /translation="MSDTSVAAPTAGLPSKPDKIQLATPHEPFDWKRLLFMGIGLGLF
                     FLVYYAPPFPDAVDPKGEIFALSKEGKAAIALFLLAGTWWVFEVVPIGITSILIGVFQ
                     ALFMIRKPDVAFSDFMAPSVWFIFGSLIIGMTFSKTGLTKRMAYKMLGIVGERTSMIY
                     LGSFLMTGAMTLVMAHTAVAAAVFPLLMAIYSLYDDGEKPTNFGKGLFIGMAFTAGAG
                     SIITLLGAARGAVAIGFFKDIAGREITFFGLTKYMLPLGVTMIIGIWLLMLLLFRPEK
                     KTIPGLKQRAQHLYQTLGPMSRHEILAIIIVLAAVFVLSATSFFPVLKAYDKSAIILV
                     ATLLFFILKILTVKDLEDVPWNIVLLFGGAMSIGFCLWQTGAAKWIAIHWLTMFQGAH
                     WFIFVMGIAFFVLVMTNFIMNVAAIAISLPVALVIAPYLGVAPEVVLYASLATAGMPF
                     MLLVGAAPNAIAYGSKQFTSGQFFRAGIPASIMLLVILGLFILFIWPLMGMPVTVAQT
                     "
     gene            complement(5213..6148)
                     /locus_tag="PK710_13380"
     CDS             complement(5213..6148)
                     /locus_tag="PK710_13380"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HOT10763.1"
                     /translation="MKALRAVYGKTLARLGAEKPEIVVLDADLSKSTKTSDFAKAFPK
                     RFHDMGIAEQDMLATAAGMATAGLTPFASTFAIFGTGRAWEQLRNSVCYPELNVKLVA
                     THGGITVGEDGGSHQGIEDLALTRVIPNLRVIVPADPMETEQVIETVASTRGPFYVRL
                     PRGSAPDIHPADYRFQIGKACVLRQGADVTLVGIGLMVKVALDAASLLAQQGIEATVI
                     NASSLKPLDSDTLEQAARATGAVVTIEEHSIIGGLGGAVAEALAERFPVPIVRQGLND
                     VFGQSGTAESLLVHYGLVPEVTVQHAKRAIALAKG"
     gene            complement(6145..>7999)
                     /locus_tag="PK710_13385"
     CDS             complement(6145..>7999)
                     /locus_tag="PK710_13385"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOT10764.1"
                     /translation="SHPSYRIGDSMESSDKKPVLVVGSSNFDLMIQTERLPGPSETLP
                     GGDYRAGPGGKGANQALGLLLLGSPVRFLSRVGNDHYGRQILAYLEEQGLDISHVSID
                     STAPTGLALVTLDAMGTRTIVVAPGANATLSEEHVDDFLKEAPDGSVLVTQLEIPVPT
                     VAHLLRLAKRNNLRTVLHASPAVTFPLALLRLVDVLVLNQSELHVISGRSAQHLETAR
                     EAADHLLAAGVGTVLVTMGTRGVLVAQRKGAWPPSMEEESRFVPGYKVNVKDGNVASS
                     AFIAGFVHHLSAPGQCEDLRAAIRFGLATMAVSLGSPRGETSMATEQETREFLSSASS
                     VVSLRPTKQGEKIQMMANRATSIRKRIIRMLFAARSGHPGGSLSIVELLTTLYFHTMV
                     YNPKEPNWPHRDRLVLSKGHAAPALYSTLIEAGFLDPALEGELRKLGSPLQGHPDLKK
                     CPGIDMSTGSLGQGLSAANGMALAARHNHGGYRVYVILGDGEVQEGQVWEAAMTAAHY
                     GLDNLCAVVDYNALQIDGNISQVKSPIEPLADKWKAFGWYVLNVDGHDLVDLCAAFDR
                     ARELKGKPTMIVAHTIKGHGVSFMEGVIEYHGSTLSEDEVRRALRELEEEG"
BASE COUNT         1675 a         2032 c         2390 g         1902 t
ORIGIN      
        1 gtatcttttc gacctgaatt atattcgttc gagtctcgaa gacattcggg cggcggtggg
       61 gcagattatc gagcaattgg ttttgttgag cgatggtcga tgtcgtcctt tgcgccagcg
      121 attcgagcag atcgatgggc aaatcgctcg tatgtttccc gattatcgaa agctcgaacc
      181 aaccgcatac gtattgtggt tcgatgagat tggccgtgag catgcgctga gtgtgggcag
      241 caagaatgcg cagttggggg agatgcgctc acgtcttggc atgacggtac cgaacggatt
      301 tgcggtgacg gcatgggcgt ataagcggtt ttttgatgcc aatgggttat cggatcgcat
      361 tcgttcgctg gtgcaaaatg cagacgtttg ctcgtttgcg agcctggtcg aggtaagcga
      421 aaaagtccgg gcgatggtga tggaggcgcg ggtgccagcc gatttgtcgg aggcgattgt
      481 ccaggcattt caacgtcttg tggccgggtc ctcgggcaag cgcgtagccg ttcgatcgag
      541 cggattggag gaagacagcc tgttcagtta tgcggggcag tacgccacgt tgttgaatgt
      601 gggtgcgagc gaagcggtat ccgcgtatcg acaggtggtg gccagcaagt acacgccaca
      661 agccatttac cattatttga gtcatgccct gggggaagcg gaactcgcga tgagcgtggg
      721 gtacgtgcaa atggtggacg cacgagccag tggcgtcgtg tatacgcgag atcctgtata
      781 tcccgatacg gatagtttat ggattcacgc ggtattgggt cttggagcgt atctggtcga
      841 tggcaccttg acgccggatg ctttcaacta ttcgcgctcg gatctggtgg agcaagagcg
      901 tcaaattgtg gaaaaaaagg ttcgattggt gctggattct tcagggggga cggtgcagga
      961 gcgagttccc gacgaagagc agacgaggcc atcggtgtcg gagaaggagc ttcgagaact
     1021 ggcgcaattg gggcttcgtc ttgaagaaca ctatggggtg ccgcaggata ttgaatgggc
     1081 catcgatgcg caaggtagtc ttcagttgtt acaaacgcgg ccattgcaac tcgtgaagcg
     1141 ttcgacgaca ccgcgaacgt tcgaggtgga tgggaaggat gtgcttttgg aaggggcaac
     1201 gacggcgagt ccgggagcgg gggctggtcc tgtttatcag gtgcgaacga gtgtcgattt
     1261 gcttggggtc aacgatggca tggttgtgat tgccccgaat ccttttccgg gtctgattac
     1321 gattatggga aaggccaggg cattggtgac cgaagtaggg ggagtggcga gccacatggc
     1381 aacgctttcg cgagaatatc gattgccgac cttggtggga gcttccggcg ccatgggcat
     1441 acccaacggt acgtcggtga cggtggacgc gtcggggggg agaattttgg cgggtctgca
     1501 tttcgatctc gccgaggcgc gcgtggcgga gacgaacaat ctttttgagg ataccccttt
     1561 atttcaaggg tttcgtcgaa ttctggacct ggtagctccc ttgcacatgc tgcacccttc
     1621 ggatccttcg ttcctgcccg agaactgtcg aaccctgcac gatatcacga gatatgctca
     1681 tcaaaaatcg atggaagaga tgtttcgtcg ggtctcgacg ttggagacaa ccgctgaatt
     1741 cggtcaaccg gtcgtcagcg agttaccttt gcccgtgcgt gtgttgtacg tcgaccgacc
     1801 catgacggat ctcggagaag gcaaaggggt gacgatgcag gagatcgaca gcgagccgat
     1861 gttggcgatc tggtcgggga tgcgagaaca aggatggcca tcggggccat cgtcgacccg
     1921 gacgccattt cccacgcgtc tcaagacccg gggcaagcgg tcggagcctt ttcaatatac
     1981 cgtgaaaagc tatgccgtat tgggcactca ttacatgatg ttgtgtttgc accttgggta
     2041 tcacttcacc acgatcgagg cgttgtgtac ggaagatgcg aacaaaaact atgttcgatt
     2101 acagtacaaa gacggtggag catcgttgga acgccgggtg cggagaatcc gtctggtggc
     2161 caccttgttg tcgcgtcttg gtttcgagaa tcaaacggaa gcggattttc tcgatgcgtt
     2221 gtattcgcat gcggattgcg agacggttca ggacaagctt cggttgttgg gacgcatcac
     2281 gatgctgacg aaacaacttg atatggcgtt gtcgagcgat gccattacgg agtggtatac
     2341 gaaagatttt ctgtcgaagt tggggcttga aagctgagga tgttccaaga cttgggatgg
     2401 aaagggtcaa tggtgagcta ttcacgatag cgaatcccgt agaaaacagt ttgagggctg
     2461 cgctatggat ttggaagtcg cgtgaggatt cacgagactt ggacccacct tagggaaggc
     2521 ccatgccaca gcatattctc gccgtcgatg atgaacctca tatgctcaag ctgttggcga
     2581 ggattgtgga ggatcagacg ccttatcaga tgacgacgac gtcgaattct ctcgaggttc
     2641 ctgcgttgct cgaggcgcag tctttcgacg tggtggtgac ggatttacgg atgcccgggt
     2701 tggatggttt cgatattgtc cggatggtga acgagcgcgg gctaggggct catgtgatca
     2761 tgatcacggc gtttggtggg atggaagcgg caatgaaagc attgtcggaa ggggtatttg
     2821 agtatattca caaaccgttt cgcaaggagg catttctgtt ctcccttcat cgtgcgatgc
     2881 aatcggccaa gacgcggcgc gaggctcttc gactcgagga gatgatagcc atggaacctt
     2941 atggggaagc acgccgggcc ttcgatcgag agtacgttcg acgtttgcag gaacgggaac
     3001 cgaatgattt atcggcgcaa gccgagcgca gtgggctcga tccgaattgg atcgatggat
     3061 tgaggcatgg ttgatccttg gtgggggctc gtagccttca accaatgaaa aggagcgatg
     3121 ccatgaagcc gaagactgcg ggagagttga tgatcccatt ggatcaatat ccacatctcc
     3181 cctactggtt tacgcttcgt caggccatag cggagatgga aaagtcggag ttcaacgtag
     3241 acggccgcaa gtctttgcct cgtgtggtgc tggtctttga cgaacggtac cagcttttgg
     3301 gattggttcg tcgacgagac attttgcgag gattggagcc gccgtttctt acgcgaccgg
     3361 cggatgacga tttcgcggcc ttgcgggatc cgaatctgct ggagctgtct tatgacaaca
     3421 tgatcgatgg gattcgtgat cgagcagaac gacctgttcg tgatgtgatg cggccctttc
     3481 gtgccacggc tgatcatgaa gacccgttga tgaagctgat ccacacgatg gtcgagcaca
     3541 acctacatct tcttcccgtg ctcaaggaag ggagtgtggc aggggttgtg cgcacggtgg
     3601 atgtgtttcg cgaggtcgcg aggttggtcc tctgagagcg tttgacgttg ggaggaatgc
     3661 tacatgagtg atactagcgt tgcggctccg acggctggtt taccatccaa accggataaa
     3721 attcaactcg ccacacctca tgagcccttc gactggaagc gcctattgtt catggggata
     3781 ggtcttggtt tgtttttcct tgtgtactac gccccgccct ttcctgatgc ggtggatcca
     3841 aaaggggaaa tcttcgcgtt gtcgaaggaa ggcaaagcgg ccattgcctt gtttttgttg
     3901 gctggaacgt ggtgggtatt cgaggtcgta cccattggca ttacctcgat cctgattggc
     3961 gtgttccagg ccttgttcat gattcgaaaa cctgacgtag ctttttccga cttcatggcg
     4021 ccatcggtct ggttcatttt cggttcgctc atcattggaa tgacgttttc gaaaaccgga
     4081 ctgaccaaac gaatggcgta caaaatgctc ggaatcgttg gggagcgaac gagtatgatc
     4141 tatctcggca gctttttgat gacgggagcg atgacgctcg tgatggcgca tacggccgtg
     4201 gctgctgccg tatttccttt gctcatggcg atttatagct tgtacgacga tggggaaaag
     4261 cccacgaact tcggcaaagg gctattcatc ggcatggcgt tcacggcagg agccggcagc
     4321 atcatcacct tgcttggagc agctcgcggc gcggtggcga tcggtttttt caaagatatt
     4381 gctggtcgtg aaatcacatt cttcggattg acgaaataca tgctccctct tggggtaacg
     4441 atgatcatcg ggatctggtt gttgatgctc cttctgtttc gaccggaaaa aaagacgatt
     4501 cctggactaa aacagcgcgc gcaacacctg tatcaaaccc tggggcccat gtcgcgccac
     4561 gagatcctgg cgatcatcat cgtgcttgcg gcagtattcg tcttgagcgc tacatcattt
     4621 ttcccagtgc tcaaggctta tgacaaatcc gccatcattc ttgtcgcgac tctcttgttt
     4681 ttcattctca agatattgac agtcaaagat ctcgaggatg tgccttggaa catcgtgttg
     4741 ttgttcggag gggccatgag catcggcttt tgcctgtggc aaacgggtgc tgccaagtgg
     4801 atcgccatcc attggctcac catgttccag ggagcccatt ggttcatttt cgtcatggga
     4861 atcgcgtttt tcgtcctggt gatgaccaac ttcatcatga acgtggcggc cattgcgatt
     4921 tctctgcccg tggctctagt cattgccccg tatctcgggg tggctccgga agtcgttctt
     4981 tatgcctcct tggcgacagc ggggatgccc ttcatgctat tggtaggcgc ggcacccaat
     5041 gctatcgctt acggcagcaa gcagtttacg tcgggtcagt tctttcgggc gggcatcccc
     5101 gcgagcatca tgcttttggt gatacttggc ctgttcatcc tgtttatctg gccgttgatg
     5161 ggaatgcccg tgacggttgc gcaaacgtaa cgaggtggtt ggctggtgtt cgttagcctt
     5221 ttgccaaggc gatcgctcgc tttgcgtgct gtacggtaac ctcgggaacc aagccataat
     5281 gaacgagtag cgattcggcc gtaccgctct gtccaaacac gtcgttcagg ccttgccgca
     5341 caattggaac gggaaatctt tctgccagag cttcggctac cgcgccgcca aggccgccga
     5401 tgatgctgtg ttcttcgatc gtgaccacgg caccggtcgc tcgagcggct tgttccagcg
     5461 tgtcggaatc cagcggtttg agactcgaag cgttgattac ggtggcctcg attccttgtt
     5521 gcgcgagcaa gctggcggca tccaaagcaa ccttgaccat caggccgatg ccgaccaagg
     5581 tcacatccgc gccttgtcgc agcacgcagg cctttccaat ttggaatcga tagtccgcgg
     5641 gatgaatatc cggggcggat ccgcgcggaa gacgcacgta aaacgggcct cgggtcgatg
     5701 ccacggtttc gatgacctgt tcggtttcca tcggatcggc agggacaatc acgcgaagat
     5761 tgggaatgac acgagtcaag gccaagtcct cgatgccttg atgactgcct ccgtcctcgc
     5821 cgaccgtgat accgccgtgg gtcgccacga gtttgacgtt gagttctgga tagcaaacgg
     5881 aatttcgtag ctgttcccag gctcggcccg tgccgaaaat cgcaaaggtc gaggcaaagg
     5941 gggtgagccc ggcggtagcc attcccgctg cagtcgcgag catgtcctgc tccgcgatac
     6001 ccatgtcatg gaaacgtttg ggaaatgctt tggcgaaatc cgatgtcttg gtcgacttgg
     6061 acaagtccgc gtccaatacc acgatctcgg gcttctcagc gccgaggcga gccaacgtct
     6121 tgccatatac ggctctcaat gccttcatcc ttcttcctcc agctcccgca atgcgcgtcg
     6181 aacctcgtcc tcgctcaagg tcgagccgtg atactcgatg actccctcca tgaacgagac
     6241 gccatgccct ttgatcgtat gcgccacgat catcgttggc ttgcctttca gctctcgcgc
     6301 tcgatcgaaa gccgcgcaca aatccaccaa atcatgccca tcgacgttga gcacgtacca
     6361 tccaaatgct ttccacttgt ccgccaacgg ttcgatgggg gacttgactt gggagatatt
     6421 gccatcgatt tgcaaagcat tgtaatcgac cacggcacat aaattgtcga gcccgtaatg
     6481 ggcggcggtc atcgcggcct cccatacttg cccttcttgc acctcaccat cgcccaaaat
     6541 gacatagacg cgatagccac catggttgtg gcgtgcggca agggccatcc cattggccgc
     6601 gctcaatcct tgtcccaagg aacccgtact catgtcgata cccggacatt ttttgagatc
     6661 cggatgcccc tgcagcggac tgccgagctt tcgaagctct ccttcgagcg cgggatcgag
     6721 aaacccagct tcgatgagcg tgctgtaaag cgctggggca gcgtgccctt tgctcaacac
     6781 caacctatcc cgatgcggcc aattgggctc tttcggattg tagaccatcg tatggaaata
     6841 caaggtcgtg aggagttcta cgatcgacag agagccaccc ggatggcccg agcgagccgc
     6901 aaaaagcatc ctgataatcc gtttacggat actcgtcgcc cggttggcca tcatctgaat
     6961 tttttcccct tgtttggtgg ggcgcaggga tacgaccgac gaggccgaag acagaaattc
     7021 tcgcgtctcc tgctctgtcg ccatcgatgt ttcgcctctc ggactgccca acgacacggc
     7081 catggtcgcc aaaccaaagc gtatggccgc tcgaaggtcc tcgcactgtc cgggtgcgga
     7141 tagatgatga acgaatccgg caatgaatgc gctggaagcc acattgccgt ccttgacgtt
     7201 aaccttatat ccaggaacga accgactctc ctcttccatc gatggaggcc atgcgccttt
     7261 gcgttgcgcc acgagaacgc cccgggttcc catggtgacc agcaccgtgc ctacgcccgc
     7321 cgcaagcaaa tggtcggcag cttcccgcgc tgtttccaaa tgctgcgcag atcgtccgct
     7381 gatcacatga agctcggatt gattgagcac caacacgtcg accagccgaa gcaaggccag
     7441 ggggaaagtc acggccggag atgcgtgcaa taccgttcgt agattattgc gttttgcaag
     7501 acgcaataga tgcgccacgg tgggaacggg aatctcgagt tgcgttacca gcacggaccc
     7561 atccggagct tccttcaaga agtcatcgac atgctcttcg gaaagcgtgg cgttggcacc
     7621 aggcgccacg acgatcgttc gcgtgcccat cgcatcgagc gtcacgaggg caaggccggt
     7681 cggggcggtc gaatcgatag atacatgaga aatatcaagg ccttgctctt cgaggtaggc
     7741 caggatctga cgaccgtaat gatcgtttcc cacgcgggaa agaaagcgga cgggggagcc
     7801 gagcaataga agcccgagag cctgattggc gccttttcct ccgggtccgg cacggtaatc
     7861 ccctccgggc aacgtttccg aaggccctgg cagccgttcg gtctgaatca tgagatcgaa
     7921 gttcgaactg ccgaccacga gaaccggctt tttatctgac gattccatac tgtctccgat
     7981 gcgatacgaa gggtgcgac
//
LOCUS       DAOSOR010000237         7988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9030_length_7988_cov_4.786966, whole genome shotgun sequence.
ACCESSION   DAOSOR010000237 DAOSOR010000000
VERSION     DAOSOR010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7988
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9030_length_7988_cov_4.786966"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="PK710_13390"
     CDS             <1..1245
                     /locus_tag="PK710_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT10765.1"
                     /translation="AKFSVDGTQPRILLGTSPSCAIQLNDPAVSRRHAALDVHGSKLR
                     VTDVGSSNGTRVGNVRVVDAYVEVGDKIRVGFTTLQVAACDETAHPILSTRDHFGSVV
                     GASVAMRRLYPLCERLAASDVSLIIEGETGTGKEVLAESLHQQGPRAGGPFVVFDCTA
                     VPPNLIESALFGHEKGAFTGAVSTRPGVFEQAHRGTLLIDEIGDLELALQPKLLRAIQ
                     RMEVLRVGSRHPIKVDVRVIAATRRDLDREIQAGRFRDDLFFRLNVARIELPPLRRRK
                     GDAELLARHFCMLLGAPADALPERLLRAFHDYEWPGNVRELRNAVSRFLALGDLADFG
                     PGLERGVHSSTDDHNEGDFLDDVVAMHLPLQDARQQVVEEFERRYVQHAIERHAGSTS
                     QAATEAGVAKRYFRLLRARYTK"
     gene            complement(1393..3288)
                     /locus_tag="PK710_13395"
     CDS             complement(1393..3288)
                     /locus_tag="PK710_13395"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HOT10766.1"
                     /translation="MSHSVFHPGRSMRWLGVTGVLSCCWIGCEVDEADVEQPQTPSIA
                     LPDLGKADGWQPALNPEDCPGGAPTLPNGKPATWTIIHYAAADNNLESALLEDINEME
                     AGHRGTPNVNIVVQLDKRTERGVWRYHIKPDTDRSTIHSSLVGYSEEEPDSGDWRTLA
                     AFGQWAAMCYPADYYAVIIGGHGSGWSGSEDDARSASVLAETQAEGQVARSIAPDDSH
                     GTSMNIEELQRGLTEIRKATKRQDDPDWLNRLAFYGSDACLMATFEVTYDLRNTATYI
                     IGSEETEPGQGWPYSTIIRDLTERPSYYAQRPHELTKSIVRYYGASYGPSGAAKKNKG
                     ITLSAVDTSSMTKAKNAFELIADALLGLAQDDAAFGEALWEARTKTYNFYGFVDMTLL
                     FDSLQTILWDQALIPKPGQHWKGDPRAKDLAQGLNTLLKDVWPDLVVQVARGEKYPQA
                     GGLSLYFPTDLCGGWTGGPKIDVYANSGIGKATAWDELLIALIEKKNGSNKTYVAKGS
                     ATLQAEELPAAEFSVDCRFSRGQFTLRGSDSGSSFSATMNAQDGLSVTRVTWSRRTEL
                     GTQWIELPWNAEPIPMPGDYAPDQAYEAHQVSIPLQTRDSSTNTTQPLPTLLSVSCPA
                     PTMKYCD"
     gene            3515..5932
                     /locus_tag="PK710_13400"
     CDS             3515..5932
                     /locus_tag="PK710_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10767.1"
                     /translation="MRRETALCLGLFAVSAPCLLLELIQTRIFSYSLHPLLAYSAIAL
                     AMLGFGLGAVALTLRPDFAGSHPHRRLSLLSLALGVSTLLVHLLFARVSTRVIPVETL
                     WVSPGWTALALLPCVIPFAIAGMITTIVLQSKLGRIGWIYFWNLAGSALGCALMMLLL
                     QPMGAERIVAFCAVAAFGAAAIFSWPDKSRLRWIGVGMALVALGLTPFADRWIAYQPD
                     SNDLLYLYAAHHKVRLEREYSRWDPVGRIEVLRHEIPQLHVLDPVSYRTITNDSGAMA
                     LLLEAGPAGWGKEIFEQSIYSTPYRLKEKPEVLVIGVGGGIDIHAALHWGAQRVVGVE
                     VSGTTLHILQGPYANYSRFHEAGDRVRLVHADGRAYARATHERFDIVQMSGVDTFTMH
                     SASSMVTAEDYLYTVDAFEDFLALLKDDGVLAVTRFGDEAMNLSCLAAAALRRHGVTA
                     PQRHIFAVQQSSTSGIVVKRTPLTHHEIETLTHVGDRKEPNGVRIPHYDDVGLKASDP
                     VNVRYPSNPTTDDRYLRFFSAMGEGRELQVLRELRNPFVPPTDDRPYYMLLGIWMASM
                     HKMHPIVMTLQAITGLIALTSLLLIAVPWLRVRRTSRVGFRVMASVVTYFFGLGVGFM
                     LLEVGLIHRTIVFVGSPAASVSVVIASILMASGLGAYVSDRLRWPWARCLAFAFGGLI
                     ATGLLLWWGADVLFGLLVGQSAWIRWLAAALAIAPGGFFAGWFFPIGLRVIGNRSEAL
                     VPWAIAINGFASVLGSLATLSLGVSFGFKTVFLIGVGAYAVAVATLLPLSWGRRAGDA
                     MKVCSRG"
     gene            complement(5988..6536)
                     /locus_tag="PK710_13405"
     CDS             complement(5988..6536)
                     /locus_tag="PK710_13405"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOT10768.1"
                     /translation="MHAIEILMGEHKRIAELLTLLNTGAERIVANRPPPRAFFEVGVQ
                     LARQYADQAHHFKEEYVLFGLLAQKHHGELDGHIERHRNQHEQCRDLLNATSSALSAY
                     EANKEEAIKIVHRNITEYVRTLRSHIRSENEVFFPLAQTTLSQADQDQLMEEYRRFEA
                     KHADSEKNLNEGLAQMRSMLEG"
     gene            6684..7070
                     /locus_tag="PK710_13410"
     CDS             6684..7070
                     /locus_tag="PK710_13410"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOT10769.1"
                     /translation="MSESMPIVRPMSEAAETAVERSRGATIQVLIGPNEGAPRFFTRR
                     FTIQPGGRIPEHRHAEIEHEQVVLEGEMVIGLGQEQHRVRAGDTIFIPAGVSHWYENR
                     STVPIRFLCMVPRSDNYQTEWLEPPA"
     gene            complement(7098..>7988)
                     /locus_tag="PK710_13415"
     CDS             complement(7098..>7988)
                     /locus_tag="PK710_13415"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT10770.1"
                     /translation="QDDAYLHEVALRSFCLDRHEVTVAAYRECVRRGDCEEPFHNNFS
                     CTWDLPERDNHPINCVDFSRAQRACLAMGKRLPTEEEWEYAARGGSENRTYSWGESAP
                     SGKLSCYNHRETCPVDAHPAGAFGLLGMTGNVWEWTSSSFVKYAGWVPAGQWRVYRGG
                     SYSRRFPKWMKTWVRNRFRPEQWGAHLGFRCAADLSGTVCPAHTTPLENDPTRCLHPN
                     ESIPRPPPTEAGPIADDAPPPVVTRSPSFDEDCQRFKPSTPHAYDIRGSSFAQRQKET
                     AKRGCSNRDVGAKFNSVCCP"
BASE COUNT         1543 a         2243 c         2310 g         1892 t
ORIGIN      
        1 gccaagtttt ccgtggatgg aacccagccc cggatcctac tcggtacgtc cccctcctgc
       61 gcgatccaac tcaatgatcc agccgtctcc cgacgccacg ccgcactgga cgtccacggc
      121 tcgaaacttc gcgtcaccga tgtgggctcg agcaacggga cgcgggtggg aaacgtgcgt
      181 gtggtcgatg cctacgtgga ggtgggcgat aaaattcgcg tcggtttcac gacattgcaa
      241 gtcgccgctt gcgatgagac cgcccatccg atcttgtcga cacgcgacca tttcggttcg
      301 gtcgtggggg ccagcgtcgc catgcgaagg ctatatccgt tgtgtgagcg actcgctgcc
      361 tccgatgtct cgctcatcat cgaaggcgaa acaggtacgg gaaaagaagt cctcgccgaa
      421 tcgcttcacc aacaaggacc gcgggcaggg gggcctttcg tggtcttcga ttgcacagcg
      481 gtaccaccga acctcatcga atctgccctt ttcggccatg aaaaaggagc tttcaccggc
      541 gccgtatcca cccgaccagg cgtcttcgag caagctcatc gtggtacctt gctcatcgac
      601 gaaatcgggg acctggaact tgctttgcaa cccaagttgc tgcgagccat tcaacgaatg
      661 gaagtgctac gagtcgggtc ccgacacccc atcaaagtcg atgttcgcgt catcgccgcc
      721 acacgacgag acctcgatcg cgaaatacag gccggcagat ttcgtgatga cctgtttttc
      781 cgtctgaatg tggcccggat cgaattgccc ccgctgcgac gccgaaaggg tgatgcggaa
      841 ctgctggcac gccacttctg tatgctgctt ggcgcgcccg ccgacgcact ccccgaaagg
      901 ctgttgagag ctttccacga ttatgaatgg cctggaaacg tccgggaatt gcgcaacgcc
      961 gtatcgcgct tcctcgcctt gggagatctc gcggacttcg gccccggatt ggaacgtggc
     1021 gtccattcat cgaccgatga tcacaacgaa ggtgactttc tcgacgatgt ggtcgccatg
     1081 catctgccct tgcaagatgc ccgtcaacaa gtcgtcgaag aattcgaacg ccgatacgtc
     1141 cagcacgcca tcgaacgaca cgcggggtcc acgtctcaag cagctaccga agccggagtt
     1201 gcgaaacgat actttcgatt gctccgcgct cgatacacca agtaatcaca cacacggaac
     1261 gcgttacgca atcgcgtccc cagtgatggg gtaacatatt gaaattacac agtattttga
     1321 attagtccgc ccttgtccga ggcgcctggc tgctggacga acccccagga agaacagttt
     1381 catccgggtc gatcaatcgc agtacttcat cgtaggggcg gggcaggata cgctgagcaa
     1441 ggtgggcagc ggttgcgtgg tgttcgtgga agaatccctg gtttgtaggg gaatactcac
     1501 ctgatgagct tcgtacgcct ggtccggtgc atagtcgcca ggcatcggta tcggttccgc
     1561 attccaggga agttcaatcc attgggttcc cagttcggtc ctcctcgacc acgtgacccg
     1621 cgtcaccgac aagccatctt gtgcattcat cgttgcagag aagctggagc ccgaatctga
     1681 gccacgcagc gtgaattgcc cacgcgagaa gcggcagtcg acggagaatt cggcagccgg
     1741 cagttcttcg gcttgtaagg tcgcggaacc tttcgccacg taggttttgt tgcttccgtt
     1801 tttcttttcg atgagcgcaa tgagcaattc gtcccaggcg gtggctttgc cgatgccaga
     1861 gttggcgtag acatcgatct tgggtccgcc tgtccaacca ccgcaaagat ccgtgggaaa
     1921 atagaggctc aggccgccgg cctgcggata cttttcccct cgtgcgacct ggaccacgag
     1981 atcaggccag acgtccttca acaaggtgtt gagcccttgc gccaggtctt tggctcgtgg
     2041 atcccctttc caatgttgcc cgggcttcgg gatcaaagcc tggtcccaaa gaatggtttg
     2101 caaactatcg aaaagcagcg tcatgtccac aaatccatag aaattgtagg tcttcgttcg
     2161 agcttcccag agcgcttctc caaacgcggc atcgtcttgc gcaagaccta gcaacgcatc
     2221 agcgatgagt tcaaaagcgt ttttggcttt cgtcatcgac gatgtatcca ccgcgctcaa
     2281 ggtgattccc ttgttctttt tcgcggctcc ggaaggaccg taggaagcgc cgtagtaccg
     2341 gacgatggac ttggtgagtt catgggggcg ctgggcataa taggaagggc gttcggtcaa
     2401 gtcgcggatg atcgtgctat atggccaacc ttggccaggt tcggtttctt ccgatccaat
     2461 gatgtaggtt gccgtattgc gaaggtcgta cgtgacttca aaggtggcca tcaaacacgc
     2521 atcgcttccg taaaacgcca accggttcag ccaatccgga tcgtcttgcc gtttcgttgc
     2581 cttgcgaatc tccgtcagac cacgttggag ttcctcgata ttcatcgagg ttccgtggct
     2641 atcatcggga gcgatgctgc gagccacctg tccttcggct tgggtttccg ccagaacgga
     2701 ggcagaacgt gcatcgtctt cgctccctga ccaacccgaa ccatggccgc cgatgatgac
     2761 ggcataataa tccgcggggt agcacatcgc ggcccactgg ccgaaagcgg caagcgttcg
     2821 ccaatcgccg ctatccggtt cctcttcgga ataaccgacg agagatgagt ggatcgtgga
     2881 tcgatcggta tcgggtttga tgtgatatct ccacacgccc cgttccgttc gcttatcgag
     2941 ttgtacgacg atattgacgt tcggcgtgcc ccggtgcccg gcttccatct cgttgatgtc
     3001 ttcgagcaag gccgactcga ggttgttatc cgccgccgcg tagtggatga tggtccaggt
     3061 tgccggtttt ccgttgggca acgtgggagc gcctcccggg caatcctccg ggttgagggc
     3121 aggttgccaa ccatcggctt tgcccagatc aggaagcgca atcgatggcg tttgtggttg
     3181 ttccacatcc gcttcatcaa cctcacaacc gatccagcaa cagctcagca cacccgtcac
     3241 gccgagccaa cgcatcgagc ggccggggtg aaaaaccgaa tgactcatat cgcctctgta
     3301 tctcaatgaa gttcggtggg tcgaatacca ccgctctccg atgataggtt ttcatcggaa
     3361 taagcgaagt gttggtaatc gtcctatctt atacgacatc agaggaaata tctttccatt
     3421 ttgaatactt ttcggtcgtt ttggttccca tgcgttgcgg ctgtggcgca gacaaaggac
     3481 ctcccacggt cgcaccctgt gctacgaccc ccccgtgcgc cgtgaaacag ccctttgcct
     3541 tggtctattt gccgtttcag ccccttgcct gctgctcgag ctgattcaga cgcggatttt
     3601 ttcgtattcc ttgcatccgc tccttgcgta ttcagcgatc gctctggcga tgttgggctt
     3661 cgggttgggt gccgtggcct tgacccttcg gcctgatttc gccggctccc atcctcatcg
     3721 acggctctcc ttgctgagcc ttgctcttgg cgtatccacg ctgctcgttc atctcttgtt
     3781 cgcgcgtgta tccactcgtg tcattcccgt cgagacgctt tgggtcagtc cgggttggac
     3841 agcgctggcg cttttgcctt gtgtgattcc cttcgccatc gccgggatga tcaccaccat
     3901 cgttcttcaa tcgaaactcg gacgaatcgg atggatctac ttttggaacc tagccggatc
     3961 cgcgctgggc tgtgcgttga tgatgctatt gcttcagccc atgggtgcgg aaaggatcgt
     4021 tgcattttgt gcggtagcag ccttcggtgc cgcggcgatc ttttcctggc ctgacaagag
     4081 tcgcctgcgc tggattggcg tgggcatggc tctcgttgcc cttggtctga ctcccttcgc
     4141 cgatcgatgg atcgcctatc aaccggattc caacgatctg ctgtatctgt acgctgctca
     4201 ccacaaggtc cggctcgaac gagaatactc acgatgggat cccgtcgggc gcatcgaggt
     4261 gttgcgtcat gagattcctc aattgcacgt cctcgatcct gtctcttatc gcaccatcac
     4321 caacgacagt ggagccatgg cattgctgct cgaggctgga cctgctggat ggggtaagga
     4381 aattttcgaa cagagcattt acagcactcc ctatcgtctc aaagaaaaac ccgaggttct
     4441 cgtcattggc gtgggcggag ggatcgacat ccatgccgcc ttgcattggg gtgcccaacg
     4501 ggtcgtgggt gtcgaagttt ccggcacgac attgcatatc ttgcaggggc cttatgcgaa
     4561 ttattcgcga tttcacgagg ctggcgaccg cgttcggctc gtgcatgcgg atggtcgcgc
     4621 ctacgcacgg gcgacccacg agcgtttcga tatcgttcaa atgagcggcg tggatacctt
     4681 caccatgcat tccgcaagct ccatggtgac cgcggaagat tatttgtaca ccgtcgatgc
     4741 cttcgaagat tttttggcgc tgctcaaaga tgatggcgtg ctcgcggtca cacgcttcgg
     4801 cgacgaagcc atgaatttgt cttgtctcgc cgcagccgcc cttcgacgac acggagtgac
     4861 cgcgccccaa cggcatatct tcgctgtcca gcaaagcagc acctccggca tcgtggtcaa
     4921 gcgcacacct ttgacccacc acgaaatcga aacgctcacg cacgtgggag accgaaaaga
     4981 acccaacggc gttcgaatcc cgcattacga cgacgtggga ttgaaagcga gcgatcccgt
     5041 caacgtgcgt tatccatcga atcccacgac cgacgaccgg tatcttcgat tcttctcggc
     5101 catgggagaa ggacgcgagt tgcaagtcct tcgagaactt cgaaatccct tcgttccacc
     5161 caccgacgat cgcccttatt atatgttgct cgggatttgg atggcgtcga tgcataagat
     5221 gcatccgatc gtcatgacat tgcaggcgat cacagggctc atcgcattga cgtccctttt
     5281 gctcatcgcg gtgccgtggt tgcgcgtacg tcgaacgagt cgcgtgggat ttcgggtcat
     5341 ggcgagtgtg gtgacgtatt ttttcggtct tggggtaggg ttcatgctgt tggaggtcgg
     5401 gcttattcac cgtaccatcg tgttcgtggg cagcccggcg gcgtccgtga gtgtggtgat
     5461 cgcatcgatt ctcatggctt ccgggcttgg ggcgtacgtc tcggatcggc tgcgatggcc
     5521 ttgggcgcgt tgcctcgcat ttgcgtttgg aggcctcatc gcgacagggc tgctgctttg
     5581 gtggggcgcg gacgttctat tcggcttgct cgtgggacaa tcggcttgga ttcgttggct
     5641 ggcagcggct ttggccattg caccgggcgg tttttttgct ggatggttct tcccgattgg
     5701 gcttcgcgtg atcggaaatc gttctgaagc gttggtgccc tgggccattg cgatcaacgg
     5761 ttttgcttcg gttttgggtt ccctggccac gctgtctttg ggcgtatcct ttggatttaa
     5821 aacagttttt ttgattggtg tcggagccta cgccgtggcg gtggcaaccc ttttgccgct
     5881 ttcatggggg cggcgcgctg gtgacgcgat gaaggtctgc tcgaggggat gaatcgatcg
     5941 tgatgggttg aaccgcggga gtgatcgcgg ttcggatgtt gctgcgatca gccttccagc
     6001 atggatcgca tctgggcaag accctcgttg aggttctttt ccgaatccgc gtgcttggct
     6061 tcgaaccgac ggtattcctc catcaattgg tcctgatcgg cttgtgacaa agtggtctgg
     6121 gcaaggggga agaagacttc attttccgag cgaatatggc ttcgaagggt gcggacgtat
     6181 tccgtgatat ttcgatgcac gattttgatg gcttcttctt tattggcttc ataggcggac
     6241 aaggccgagg atgtggcgtt gagcaggtca cggcattgtt cgtgttgatt gcgatggcgt
     6301 tcgatatggc catcgagttc gccgtggtgt ttttgcgcga ggagcccaaa gagcacgtat
     6361 tcttctttga agtggtgagc ttggtcggcg tattgtcgag cgagttgaac accgacttcg
     6421 aaaaacgctc gcggaggagg ccgattggcg acgatccgtt cggctcccgt gttcagaagg
     6481 gtgagcagtt cggcaattct tttgtgttca cccatcaata tctcaatggc gtgcatggtg
     6541 acctcccgca attggttgga ggaccgcaac atggacttgg acgggcggtt cgtcaattgg
     6601 ctgctgtttc gtcatcgtaa caagatgggg gaaacccatc cgtggccgtt gacacgggca
     6661 tcacgacgta caaaaacccg gccatgagtg aatcaatgcc gatcgttcga cccatgtcgg
     6721 aagccgccga gactgccgtg gaacgaagtc gcggcgcgac cattcaagtg ctcattggcc
     6781 caaacgaagg agcgccgcgg ttttttaccc gacgattcac cattcaacca ggtggacgca
     6841 ttccggagca tcgtcacgcc gagatcgaac acgagcaggt cgtgttggaa ggcgaaatgg
     6901 tgatcggact aggacaggag cagcataggg tgcgggcggg tgataccatt ttcatccccg
     6961 cgggcgtttc ccattggtac gaaaatcgtt ccaccgtgcc cattcgtttt ctttgcatgg
     7021 tgccccgcag tgacaactac cagacggaat ggctcgagcc tcccgcatga cccgcctcga
     7081 aaacggtaga tgcccgttca ggggcagcaa acgctgttga atttggcgcc aacgtctcga
     7141 ttcgagcacc cccttttcgc tgtctccttc tgccgctggg caaaggatga gccccgaatg
     7201 tcgtaggcgt ggggtgtgga tggcttgaaa cgttggcaat cttcatcgaa agaaggcgag
     7261 cgggtgacga ccggcggggg cgcgtcatcg gcgatgggtc cggcttccgt cggaggcggt
     7321 cgaggaatgg attcgttggg gtggagacat cgggtgggat cgttttcaag cggtgtggtg
     7381 tgggcaggac agaccgtccc ggaaagatcg gcggcgcagc ggaatccgag atgcgctccc
     7441 cattgctccg ggcggaaacg gttgcgtacc caggttttca tccacttggg gaaacgtcgc
     7501 gaatagctgc cccctcgata cacacgccac tgcccggcag gaacccagcc cgcatacttg
     7561 acaaaagaac tcgacgtcca ttcccacacg tttccggtca tgcccaaaag accaaaagca
     7621 cccgcgggat gcgcgtcgac aggacaggtt tcacgatgat tgtagcaaga cagcttcccc
     7681 gagggtgcgc tttcacccca agaataggtg cggttttcgc ttccccctcg tgcggcatac
     7741 tcccattctt cctccgtggg cagccttttt cccatggcca aacatgcgcg ttgggcgcga
     7801 gaaaaatcca cgcagttgat gggatgattg tctctttccg gtaggtccca ggtacacgaa
     7861 aaattgttgt gaaacggttc ctcacaatct ccccgtcgca cacattcacg gtaggcagcc
     7921 acggtcacct cgtgccgatc gagacaaaac gatcggagtg ccacctcatg aaggtaggca
     7981 tcgtcttg
//
LOCUS       DAOSOR010000238         7964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9065_length_7964_cov_4.260463, whole genome shotgun sequence.
ACCESSION   DAOSOR010000238 DAOSOR010000000
VERSION     DAOSOR010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7964
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9065_length_7964_cov_4.260463"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(750..1841)
                     /locus_tag="PK710_13420"
     CDS             complement(750..1841)
                     /locus_tag="PK710_13420"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOT10771.1"
                     /translation="MHERSHVSLAELTTIGLGGPAKRLIDIDDETELEQVLGELANES
                     DEVFILGGGSNVIVSDGGWPGTVIRPAFRRFDATKEGCEVLLHLGAGEVWDDVVRECV
                     QRRWAGVSCLSGIPGWVGASPIQNIGAYGTELSQVLVEVHAFDRDAKRHVIFPAADCA
                     LGYRSSRFRGNARHVIEKVVLRLRADGGDIIRYPELARSLGVREGASVNPAEVREAVL
                     ELRRGKGMVVDPRDTESRSAGSYFVNPIIDAQALQRLEARALELGIVTEAGLVPRYPN
                     PDGRFKVPAAWLIEKAGFHKGMTVGGVGISKKHALALVNRGGATSDLLRLEASIFHRV
                     MDRFGVRLQREPIFVGEEMEDTPKSCAGP"
     gene            complement(2029..3210)
                     /locus_tag="PK710_13425"
     CDS             complement(2029..3210)
                     /locus_tag="PK710_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HOT10772.1"
                     /translation="MKTRWSLAVGLGILAFSATSSATPPTETAKDIVCRGKSGEGFSY
                     AWGGECWCGQGCKPDLTNCEPGKCTPKAGSTGCPECTHSGKYGADCSGFVSKAWQVPK
                     PFPVEACDVDRYVASSFTKNHEFWNVVPMSSLQPADAAASSTHVILVIGEKDSQGNHE
                     VVEAKGCNYGIVRQSRSFSSSYNGARRINITTCECASGEEQSRECGDCGTEHRSCADG
                     CSWSAWSACEGPDPTGEQASCTVEGAKGACAVGVKQCVAGWLTCQAAQATEEICDGID
                     NDCDGEIDNGTPESLGEGKACNHTCGRGETVCIDGALQCVMPGASTPNDPCDSGVGDS
                     GVGGQDGSPTSEINSPGTWHEPESVSGCACHVGSARGNGSLWGLLAAAAAVWGFRRRS
                     R"
     gene            complement(3207..4178)
                     /locus_tag="PK710_13430"
     CDS             complement(3207..4178)
                     /locus_tag="PK710_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10773.1"
                     /translation="MRLRPNAVLTVILASCTLSLVAPRTAEAEPIVSHDPMTWVFPWD
                     KTVGRIIGQEADSEGPKSFVIKPDGGALILDQVNCRVLDTDPKGNLIQTLALPAPTFD
                     DIELVNENVMVLADRLVSKTLLLMDLQGHPLQQIEIQGRGITHSGLVTAMIARTDGVW
                     LEVEHRHSVKVLDRALQPCERQIITGRPVANGWNLQGKLDGRGEAIVSIGRRIHRETM
                     RSVSLKGQLPIRRIVWLDMDAKGQVHVVLHEAQFATESPYPVQKEQYAWVVLDESLQE
                     ISRTISPWVLTLYDQRVECRLGPDGRMWQMAFDTNGVRLIDWGRGTP"
     gene            complement(4280..5425)
                     /locus_tag="PK710_13435"
     CDS             complement(4280..5425)
                     /locus_tag="PK710_13435"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742118.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOT10774.1"
                     /translation="MAEVIVTQAPDGEEMSLNMGPHHPSTHGVLRFILHTDGEIISRA
                     IPDVGFLHRSIEKIGERNTLAGFMPYTDRVDYLAAMFCNEVWAIAAEKLLAVEVPPRA
                     QYLRCIAGELNRIISHLIGLGCMAMDLGAFTPFVQALREREFVNDFIEELCGARLTYN
                     YCRIGGVSHDVPNDTWKSRVLEWIEHFLPMVDEFDRLITYNEIFVQRCANIAVISGKE
                     AIDWGLVGPNLRGSGVPRDVRRDDPYGVYPQLEFEIPIGRGKMGTVGDCFDRFLVRVD
                     EMRESAKIVRQALLRMDEHPKGEIRGTLPKKLKFDGEVYAHVESARGDLGCYVIGHGQ
                     EQPYRVRFRTGSFTAMGIIEPKSPGLFVADMVALISSFDVVAPEIDR"
     gene            complement(5473..6210)
                     /locus_tag="PK710_13440"
     CDS             complement(5473..6210)
                     /locus_tag="PK710_13440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOT10775.1"
                     /translation="MDSKNIFEALQAKFGEDVVFGYTPASLASTDDYFFVSPDGIVEV
                     ARHLRDEPTLAFDFLECLTGVDYPDAFVRTPQEEQTSEKGTIHVVYHLRSYDKKHRAV
                     MKASLSRTDPRIATLCPIWSAANWQERECFDLLGVHFEGHPDLRRIMLPDDWVGHPLR
                     KDYEEAPFYQGIPTTRPNPLAMLAKKAASEAAKPAAKAKAEPQATAKEKPKAAAKTQE
                     KPVSESATAKEAEKQDKSKDDGDTIGS"
     gene            complement(6237..6767)
                     /gene="nuoB"
                     /locus_tag="PK710_13445"
     CDS             complement(6237..6767)
                     /gene="nuoB"
                     /locus_tag="PK710_13445"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF011393.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HOT10776.1"
                     /translation="MEARYAGEVGSVIQTNVDQTLNWLMNWGRAHSIWYLLFGLACCA
                     IELMQTGGPRADFERFGAAPRPSPRSSDLIMIAGTLTYKMALRVRRLFDQMSEPRYVI
                     SMGSCANCGGLFQYGYSVVKGVDQILPVDVYIPGCPPRPEALLEGLIKIQQKIKSEKY
                     LQERYGASRSVSKTSR"
     gene            complement(6798..7262)
                     /locus_tag="PK710_13450"
     CDS             complement(6798..7262)
                     /locus_tag="PK710_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOT10777.1"
                     /translation="MLFEYASVGAFFLIAILFVFGALLFGRLIRPNRMYSKKLETYEC
                     GEAPVGQAWFNFNPRFYIVALIYIIFDIEIAFIYPVATVFKQWVTQGMGWFALVEIIM
                     FIGILLIGLAYVWMKGDLEWIRTLRSDRRGPQDVRSDGSHPNALPETAPQEI"
     gene            7503..>7964
                     /locus_tag="PK710_13455"
     CDS             7503..>7964
                     /locus_tag="PK710_13455"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOT10778.1"
                     /translation="MTSSHFAVIMAGGSGTRFWPASRHRNPKQFLALGPNPSEPLLLG
                     TVRRMTAVVPPSNILIATGAHLVEHTRQCLPFLPTENILAEPTARNTAPCIAWANAVI
                     RRRDPNAIVAVLSADHVATDEVGFQSAVTHAMQVAASGIITTLGIQPTRPEA"
BASE COUNT         1672 a         2429 c         2144 g         1719 t
ORIGIN      
        1 gttgccgacg ttccaccctt gccagcagtt cctcctccgc cctgctcagg gccgtcgtcg
       61 ctaccacagc agaccacggt gaaaacggct gccgcggtca tcaggatgcg cagcacggga
      121 agccttcgaa gacgttcatg cgatgttttc atgcgatcac ctgttgattc cctcatcgtt
      181 gtcacggcgc gcagccaatg cgcacgagta tgtcgaactc agagcggaca gcaaacgttc
      241 gtgggtgcgg cagaccaaac gcatggatta agggatcaat gtgacaccca aaacggaggc
      301 ttatcaacgc aattcgttgc ggagggctcc gccggtcgtg gcgcagggac aatcgtagcg
      361 agtgtcgtgc ggtctatggc ctgtagcgtt tctctgttct tgcttttctc acggtcttat
      421 ggcgtcgcca gccctccact ccggcccatc ccccacgccg cggggctggg aacgagaagc
      481 agggtagtac aagcgccacg caacgagaaa actgtgcttt cgcaggccga tggttcgttc
      541 gccgagccac cgctcggcaa acatatcgca ccgcagaatg gtttttgctc atagaaggga
      601 gccacgacgg cctggctgaa ttaccgatgt gactccgagc gcaatctccg gaggatttgt
      661 ctcgacagga attgtaattt cgctttattt tcaataggtt atattctgtg aagccttcgt
      721 gaaccccttg tccgggaatg ctttcccgat catggccccg cacaactctt cggcgtatct
      781 tccatttcct cccccacgaa aatcggctct cgctgaaggc gaacgccaaa cctatccatg
      841 acccgatgaa aaatactcgc ttcgagacga agcaagtccg acgtggcccc gccacggttg
      901 accagagcaa gcgcatgctt tttcgaaata cccacgccgc cgaccgtcat acctttgtga
      961 aatccagcct tttcgatgag ccaggccgcg ggcactttga accggccgtc ggggtttgga
     1021 taccgcggca caagaccagc ctcggtaacg attccaagct cgagagcccg cgcttcgaga
     1081 cgttgcagcg cctgggcatc gatgatcggg ttgacgaaat aggaacccgc gctgcgcgat
     1141 tccgtatccc gtggatccac gaccatccct ttgccacggc gtagttcgag caccgcttcg
     1201 cgaacctccg cgggattcac cgatgctccc tcccgtaccc caagcgaacg agccagttcg
     1261 ggatagcgaa tgatgtcacc gccatcggcg cgcaaccgaa gcacgacttt ttcgatgacg
     1321 tgacgagcat tgccccgaaa cctgctgctt cgatatccca acgcgcagtc ggctgcggga
     1381 aatatgacgt gccttttcgc gtcgcgatcg aaggcgtgta cctcgacgag cacctgagac
     1441 agttccgtgc catacgcgcc gatgttttgg atgggcgatg cgcccaccca tccgggaatg
     1501 cccgacaaac aagacactcc cgcccatcgt cgttgaacgc actctcgcac gacgtcatcc
     1561 cagacttccc cggcgcccag atgaagcaag acttcacagc cctccttggt cgcatcgaac
     1621 cgacgaaaag ctggacgaat caccgttccg ggccaacccc catcggaaac gatcacgttg
     1681 ctgccgccgc cgagaatgaa tacctcgtcg gactcgttcg ccaattcgcc gagaacctgc
     1741 tcgagttccg tttcatcatc gatatcgatg aggcgttttg cgggaccacc caggccgatg
     1801 gtggtcagct ccgcaaggga aacgtgcgaa cgctcatgca tgcgacggtc gtagaatgcc
     1861 gtggctgcct cgggcaagtc cttggcaaaa ttgtcttcgt gtgatgggcg atgattcgat
     1921 tcccaccgtt gttggagaaa atacggatga tgatcgtttt gtttcgggcg ttgcggagct
     1981 tatcgcgatc gcggcctggg cggatggttc ttgcaaagcc ctaatcgctc atcgggaacg
     2041 tcgtcggaag ccccatacgg cagccgcggc ggccaacaat ccccacaggc tcccattgcc
     2101 acgggcgctg cccacatggc atgcacaccc actgacggac tcgggttcat gccacgtgcc
     2161 aggagaattt atttcagaag taggtgagcc gtcctgcccc ccgaccccgc tatccccgac
     2221 tccgctatca caggggtcgt tgggtgttga ggctcctggc atcacgcatt gcaatgcgcc
     2281 atcgatgcag accgtttccc ctcggccgca cgtgtgattg caagcctttc cttccccaag
     2341 ggactccggc gtgccgttat cgatctcccc gtcgcagtcg ttgtcgatgc catcgcagat
     2401 ttcttccgtg gcttgcgcgg cctgacatgt caaccatccc gccacgcact gcttcacacc
     2461 gaccgcacag gcgccctttg ccccttcgac ggtgcaactc gcctgctctc ccgttggatc
     2521 gggtccttcg caagccgacc aagcagacca ggaacaaccg tccgcacagc ttcgatgttc
     2581 cgttccgcag tcaccgcatt cacggctttg ctcttcaccg ctggcgcatt cgcacgtggt
     2641 gatgttgatc cttcgggcgc cgttatagct cgacgaaaag ctcctgcttt gacgaacgat
     2701 gccgtaattg cagcccttgg cttccaccac ctcgtggttg ccctgactgt ctttttctcc
     2761 aatgaccaag atcacatgag tgctgctggc ggccgcatcc gcgggctgca agctgctcat
     2821 cggaacgaca ttccaaaact cgtgattttt cgtgaagctc gacgccacat accgatccac
     2881 atcgcaagct tctaccggga aaggcttggg tacttgccat gctttggaga cgaaacccga
     2941 gcaatccgcg ccgtacttgc cgctatgggt gcattccggg cagccggtgg accccgcttt
     3001 cggcgtgcat ttgcccggtt cacagttggt caggtcaggc ttgcatccct ggccacacca
     3061 acactcccct ccccaagcgt aggaaaatcc ctccccggat ttgccccgac acacgatatc
     3121 tttcgcggtt tccgtaggcg gcgtggcgct cgacgtcgcc gaaaaagcca gtatcccaag
     3181 acctacggca aggctccatc gcgtcttcat ggcgtccccc ttccccaatc gatcagccgg
     3241 actccgttcg tgtcgaaggc catctgccac attcgcccat ccggccccaa tcgacactcc
     3301 acacgttgat cgtatagggt caatacccag ggactgatcg ttcgcgagat ctcctgcaat
     3361 gactcatcca gcacaaccca tgcatattgc tccttttgca caggataggg cgattctgta
     3421 gcaaattgcg cttcgtgcaa caccacatga acctgcccct tggcatccat gtccagccat
     3481 acgattcgcc ttataggaag ctgtcctttt agggatacgg aacgcatggt ttcgcggtgg
     3541 attcgacgcc cgatcgatac gatggcctcg cctcgtccat cgagttttcc ttgtaaattc
     3601 catccgttag ccacagggcg tcccgtgatg atctgccgtt cacaaggctg cagggctcgg
     3661 tcgagcactt tcacgctgtg tcgatgctcg acttccagcc ataccccatc cgttcgcgcg
     3721 atcatcgccg tgaccagtcc actatgcgta atgccccttc cttgaatctc gatttgttgg
     3781 agagggtgac cttgtaagtc catgaggagc agcgttttgg acacgagccg gtcggccagc
     3841 accatcacgt tttcgttcac caactcgata tcgtcgaagg taggcgccgg gagcgccagc
     3901 gtttggatga ggttgccctt cgggtcggta tcgagcaccc gacagttgac ttgatccagg
     3961 atcaaagctc ccccatcagg tttgatgaca aaacttttgg gcccctcgct atcggcttct
     4021 tgtccgatga tacggccgac ggtcttatcc catggaaaga cccaggtcat tgggtcgtgc
     4081 gagacgatgg gctcggcttc ggccgtgcgt ggagcgacaa gacttagcgt acaggacgca
     4141 agtatcacgg ttaggacagc gtttgggcgc agacgcatgt ttcacctctt cgttctgctc
     4201 tttacggcct ttcgcaatca tagcgcaaaa ccgggcaaga ggtggcccag cattgacaaa
     4261 ggcaaggtgt attgagcagt cagcggtcga tttcgggagc gacgacatcg aagctggaga
     4321 tgagggcgac catatccgct acgaacagac cggggctttt gggttcgatg atgcccatgg
     4381 cggtgaacga accggtgcgg aagcggacac gatagggttg ttcttgtccg tggccgatga
     4441 cgtagcagcc caagtcaccg cgagcgcttt ccacatgggc ataaacctcg ccatcgaact
     4501 tcagtttctt gggcagggtg ccccggattt ctcccttggg atgctcatcc atgcgcagca
     4561 acgcctggcg aacgatcttc gcggactcgc gcatctcgtc gacacgaacg agaaaacggt
     4621 cgaagcaatc gccgacggtt cccatttttc cgcgtccgat ggggatttcg aattcgagtt
     4681 gcgggtagac cccgtaaggg tcatcgcgac gcacgtcgcg tgggactccg cttcctcgca
     4741 agttggggcc gaccagtccc caatcgatgg cttctttgcc ggaaataacc gcgatattgg
     4801 cgcatcgttg cacgaagatc tcgttgtagg taatgagccg atcgaactca tcgaccatgg
     4861 gaaggaagtg ttcgatccac tccaggactc ggcttttcca tgtgtcgttg ggcacgtcgt
     4921 gggagacgcc accgatgcgg caatagttgt aggtgaggcg cgctccgcac aattcctcta
     4981 tgaaatcatt gacgaattcg cgctcccgca gcgcttggac gaagggggtg aacgctccca
     5041 ggtccatggc catgcagcca aggccgatga gatggctgat gatgcggttg agttctcccg
     5101 cgatgcatcg caggtattgg gctcgtgggg gaacttcgac tgcgagcaac ttttcggcag
     5161 cgatggccca cacctcgttg cagaacatcg ccgccagata gtccacgcgg tcggtatagg
     5221 gcatgaagcc cgcgagggta tttcgttccc cgatcttctc gatggaacgg tgcaaaaaac
     5281 ccacatcggg aatggcacgg ctgatgatct cgccgtccgt gtgcaggatg aatcgcaaca
     5341 cgccgtgggt cgacgggtga tgcgggccca tgttcaggct catctcttcg ccgtcgggcg
     5401 cctgggtcac aatgacttca gccatgattt ccactcgagt tcggtttcgt cgatccggtc
     5461 gggttggggg cttcaggacc cgatggtatc gccatcatcc ttggatttat cttgtttttc
     5521 cgcttcctta gccgtagccg attcggagac aggcttttcc tgggtttttg cagcagcttt
     5581 cggcttttct ttggctgtgg cctggggttc ggctttcgct ttcgcagcag gtttggcagc
     5641 ctccgatgcc gctttcttcg ccagcatggc gagaggattg ggacgcgtgg tgggaatacc
     5701 ctggtaaaaa ggcgcctcct cataatcctt gcgcagcgga tgcccaaccc aatcgtcggg
     5761 aagcatgatg cgacgaaggt ccggatgccc ttcgaagtga acgccaagaa ggtcaaagca
     5821 ttcgcgttct tgccaattgg ccgccgacca gatgggacat aaggtggcaa tccgagggtc
     5881 ggtgcgggac aacgatgctt tcatgaccgc ccgatgcttc ttgtcgtagc tccgcaaatg
     5941 gtagaccacg tgaatcgtcc ccttctcgga ggtctgctcc tcctgcggcg tgcgcacgaa
     6001 agcgtcggga tagtccacgc ccgtcaaaca ttcgagaaag tcgaaagcga gcgtgggctc
     6061 atcacgaaga tgccgcgcca cctccacgat tccgtcaggg gaaacgaaaa aatagtcgtc
     6121 ggtcgaagcc agactggcag gggtgtagcc gaagaccacg tcctcgccga atttcgcttg
     6181 aagggcttcg aagatgtttt tggaatccat ctgtcgttcc gcttatcgct aaaggctcat
     6241 cgggatgttt tggagacgct ccgagaagcc ccgtaacgct cctgcaagta tttctcgctc
     6301 ttgatttttt gctgaatctt gatgagcccc tcgagaaggg cctcgggcct cggaggacat
     6361 cccgggatat acacatccac cggaaggatc tgatcgacac ctttgacgac cgaataaccg
     6421 tattgaaata gccctccgca gttggcgcaa gaccccatgg aaatgacata tcgcggttcg
     6481 gacatctgat cgaaaaggcg tcgaacacgc aaagccatct tatacgtcaa cgtcccggcg
     6541 atcatgatga ggtcgctcga acgaggactc ggacgaggag cggctccaaa acgctcgaaa
     6601 tcagctcgcg gaccaccggt ttgcatcagc tcgatcgcac aacatgcaag accaaatagc
     6661 aggtaccaaa tgctgtgagc acgaccccaa ttcatgagcc agttgagcgt ttgatccacg
     6721 ttggtctgga tgaccgagcc gacctcgccg gcatagcgag cttctatgcc agtcgtcgaa
     6781 tgggttgaag aagtcaccta gatctcctga ggtgccgttt caggcaacgc gttgggatga
     6841 gagccatcgc tgcgaacgtc ttgagggccg cgacgatccg aacgcagcgt tcgaatccat
     6901 tcgagatcgc ctttcatcca tacgtaggcc aaaccaatca gcaaaatgcc gatgaacatg
     6961 atgatttcca ccaaggcaaa ccaccccata ccctgggtta cccactgttt gaaaacggtc
     7021 gcaacaggat agatgaacgc aatctcgata tcgaagatga tgtaaatcag cgccacgata
     7081 taaaaacgtg ggttgaagtt gaaccacgct tgccccacgg gtgcttcgcc acactcatac
     7141 gtttcgagct tcttgctata catacgattc ggccggatca atcgaccgaa aagaagcgct
     7201 ccaaacacga agagaatcgc gatcagaaaa aacgcgccta cactcgcgta ttcgaacagc
     7261 atctcatcgt ccttccacgt tcgcgcttcg ctcgcgaact tgcacgaccg cccgctcgaa
     7321 agcgcgacgg tacggaagga aacttaggag acatgggggc caagggtcaa gggtagcggc
     7381 accatccctg cccgcctacc gctcccccaa cgaacgatcc gtcatgggaa gccagattca
     7441 atgacaattc cgggctttcg ccgttgctcc cttgccctat tatgcctaat ttgaggaaaa
     7501 ctatgacgtc cagccatttc gcggtcatca tggccggtgg gtccggaact cgcttctggc
     7561 ccgcctctcg ccatcgaaat cctaaacaat tccttgcctt gggccccaac ccctccgaac
     7621 ccctgttgct cggaaccgtg cggcggatga ccgctgtcgt ccccccatcc aatatcttga
     7681 ttgccaccgg tgcccatctc gtggagcaca cgcgccaatg cctccccttt ttgcccacgg
     7741 aaaacatcct tgccgaaccc accgctcgca atacagcccc ctgcattgcg tgggcaaacg
     7801 ctgtgattcg acgacgcgac cccaacgcca tcgtggcggt cctgtccgcc gatcatgtag
     7861 ccacggacga agttggattt cagtccgccg tcactcacgc gatgcaagtg gccgctagcg
     7921 gaatcatcac aacgctcgga attcaaccga ctcgaccaga agca
//
LOCUS       DAOSOR010000239         7963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9068_length_7963_cov_4.542362, whole genome shotgun sequence.
ACCESSION   DAOSOR010000239 DAOSOR010000000
VERSION     DAOSOR010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7963
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9068_length_7963_cov_4.542362"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            677..1834
                     /locus_tag="PK710_13460"
     CDS             677..1834
                     /locus_tag="PK710_13460"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOT10779.1"
                     /translation="MIAVAPMPELTDLILEVGGHELQVCMQCGTCTAVCPWPRVKPFS
                     PRQVIRYMAFGFEGFEQEDLWNCVTCRTCVQRCPRGIDLLEVMRAARAVMLESGSGPR
                     TFSGPLASLRTEGNPWAGERQERYGFAAPRPVPPYDTDKDYLFFSCCTQSYDPRNRRV
                     GTAMLDILNTAGVRYGCLGAAESCCGDQARRIGAQDVYDDVKQQNMTLFEEHGVRELI
                     VASPHCLQTINQDYAKGTELHATHYATFFHRLVQDGHLILSKPIEAKVTYHDPCYLGR
                     HAGEYEAPRELLRAIPGLQLLEMPRNRERSLCCGGGGGGIWLDVPSEERFSVLRVQEA
                     LRTGAEIIATACPYCTAMLEDAIKNLDVEHALRVLDIAELVAQSMTTEAGQ"
     gene            1840..5235
                     /locus_tag="PK710_13465"
     CDS             1840..5235
                     /locus_tag="PK710_13465"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT10780.1"
                     /translation="MQERIGFYICHCGINIASRVRCPEVAEYVATLPDVVVSRDYLFM
                     CSDPGQELIEKDIPAYGLTRVVVASCSPRMHENTFRGAVARGGLNPFRCFHHVCVREH
                     VSWVHTDMDEATEKAKTLVRAGVRRVARQADLFPNTFPVNPNTLIVGGGIAGMQAALD
                     IASAGYHVYLVEKQPTIGGHMLQYDKTFPTLDCAACIGTPKMVSVGQNKNIDLLTYAE
                     VEELSGFIGNYTARVRKKARYIDASKCTGCGECTKVCPVDKPNEWDVGTLKRHAVYRS
                     FPQAVPITFVIDKNDRAPCVQTCPAQTNAQGYIALIKAGKYLEATQLILEKLPFPGSL
                     GRVCPAPCEAVCRRKKVDEPLSIRNLKRFAADQVDWDSLPLPPIEKKSEDDRVAVIGS
                     GPAGLSAAYFLARMGYPVSVFEALGVAGGMMRTGIPDYRLPPAILEREIKYIERLGVN
                     LHLNTPIGKDRTVDDLFHQGFRAVFVATGTHGDAKLGIPGEDAQGVMAGVAFLKRQNL
                     AGDARVGEDVVVIGGGAVAIDVARVARRIGAKRVRLYCLEACHEMPAWEEEVHAAKAE
                     GIEIHNTWGPAQILVSEGKARGIEFKRCTRVFDDKKRFSPAYDEAQRQEVCCDTVLVA
                     IGQRPDTSWAEGSNVPLHPRGYVQADERTFATERPGLFAGGEVYTGPSIVVQAVANGH
                     EAAISIDRYLRGEDLLEARPERPKGEQWNPIPDDALPTPRAKMPEIALDQRQGFEEVE
                     LGFTEAQALEEASRCIACGTCSECMLCVANCKAQAIDHSMKDEVLNLDVGSVIVATGF
                     DPLDPTPMHEYGYGKYSNVYTNLEFERLSNATGPTAGALLMRDPENRYHFTSPPSSVA
                     ILHCIGSRDVNHHEYCSRTCCMYALKYAHLLKDKVGHDVLVYNFYIDMRCFGKGYEEF
                     YRRIQGEGVRMVRGKATRVTDEAESPEEEGKLVVEAEDTLTGKLLRVPVDMVVLCTAM
                     EPRKDTVDVARTFGISIGGDGFFLEEHPKLEPVSTATAGVFLAGACQGPKDIPDTVAQ
                     AKAAASMAQALTSLGRVEVQPMVSSIDEDICVGCKVCIGLCPYSAIEFDDRRGISVVN
                     DAVCKGCGSCAGYCPSGAAKIRHFTDRQIFGEIEGLLVARSAS"
     gene            5232..5660
                     /locus_tag="PK710_13470"
     CDS             5232..5660
                     /locus_tag="PK710_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOT10781.1"
                     /translation="MSEKQFEPNIVAFLCNWCTFTAADLAGTSRLIYPSNVKIIRMMC
                     SGMVDPKYVLKSFLEGADGVFIGGCWPGDCHYINGNMKARRRVALMHEVLERFGIERD
                     RLWLRWVAASEGNAFADTVTAMTGRLKELGPCRVSREAVA"
     gene            5704..6819
                     /locus_tag="PK710_13475"
     CDS             5704..6819
                     /locus_tag="PK710_13475"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOT10782.1"
                     /translation="MGQYTAIQTEGHINQAIADVLGKLLDKGVVSAVLVPCQQPHRKA
                     VMQTLVRDPEALAHIDPFAPVVPTNSARLVATLTHHNDAPVAAVVRSCEVRAFLELVK
                     LHQGCADNLLIIGIDCYGRYENKDYFALAAHQEDLTTRFLMSMASGAGGSDGVVLGAP
                     LANACRMCEYPVAEPVDIRLCAIGTDVTQQVWFEWVSEKGLRAMETLALPSQEQGPQA
                     RESAIAKLRKERMAHRDAELAAFRSKTDTMTGLADVVAACINCYNCRVACPACYCKEC
                     VFVTDTFRHDGEQVMRWAFHRGHLKMPTDTVFFHVTRMLHMSTLCVGCGQCSSACPND
                     IPVMELFRSVAERTQARFAYVPGSLLTRKTAAGGVSR"
     gene            6864..7916
                     /locus_tag="PK710_13480"
     CDS             6864..7916
                     /locus_tag="PK710_13480"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT10783.1"
                     /translation="MVAVVEDKTNLAEESPSFGSVLIVGAGVAGIRAALDLAQVGYRV
                     WMTDASPALGGILSKLDYQFPNDHCGMCKMLPLVGREQASQFCMRKSLFHDNIEILPF
                     TDVVSIRGEPGAFQVDLLEHARWVDSDVCIGTGLCASVCPLEAPEAFNQGLTRRKAIY
                     RPVPHNLPNLWAIDAELCDRCGKCVEICPVGAIHLDAPDKRSTLAVDAVIVATGTGLY
                     DPSQQGDLSSYGLSPDVVTSLQFERLLSGTGRYDGELRRPSDGRVAKKIAWLQCVGSR
                     NRAQGRDYCSSICCMFALKEAVLAHEKGGEGVDTAIFYMDMRTFGKDFQRYRQQAEQK
                     HGVRLIRCRVHTVDKT"
BASE COUNT         1672 a         2259 c         2361 g         1671 t
ORIGIN      
        1 aaaaaacttg gccaactatc ttgcgttctt tcttcccaaa gtacgccccc atgctgtcgt
       61 caccgatacc ggaagcgatc ttccatcctt ggtgcggatt ctgactcttc gatcgacaag
      121 cttttgtcca gattccagag tgcgacgtaa aggacagact tgatcgcata agtcgcttcg
      181 caacacctcc cggcatggac gaccaatcgc ctcttccggg gaaaatcctg tcgtcgtttc
      241 cgcactacga ttgaaagagg taatgatccc attgggatct accgtgaata ctccttgatc
      301 aatcacatca aggagcagag gaaaatatct tgggtcgagt ttacccacgc gagacatctc
      361 cccactttgc tccaagtgtc gcagatacgc aagctgtcgt gcgacagtgt cgcaatccgc
      421 tacagcagcg cccgatgaac cggaccgaat attgacgact gtttctcatt ctgtccacgg
      481 aattcaaacc tcactttcga ttcctaagca cagggcgtgc cgttctggcc cgttggcacg
      541 tctcatgctc acccgctgcg gccaaagcga gtgtgctcgg tccatcgaat agcacactcg
      601 gttttcgcat cgactttcgt gcgaatttca gatttcgcga aggggggcgt gtcccttctt
      661 cgtggaggaa gactcgatga tagcggttgc cccgatgccc gagctaaccg atctcatcct
      721 ggaggtcggc ggccacgaac tgcaagtctg catgcaatgc gggacatgta ccgcggtatg
      781 cccgtggccc agggtcaagc ccttcagtcc tcggcaggtc atccggtata tggccttcgg
      841 cttcgaaggc ttcgaacagg aagacctatg gaattgcgtg acctgtagaa cttgtgtgca
      901 gcgatgcccc cgcggaatcg acctgctcga agtcatgcgc gccgcccgcg ccgtcatgct
      961 cgaatcgggc agcggacccc gcaccttctc cggccccctc gccagcctgc gaaccgaagg
     1021 caatccctgg gccggtgagc ggcaggaacg ctacggtttc gcggccccgc ggcccgtgcc
     1081 cccctacgac accgacaagg attacctatt ctttagctgc tgcacccaat cctatgaccc
     1141 gcgcaatcgt cgtgtcggca ccgccatgct cgatatcctg aataccgcag gcgttcgcta
     1201 tggctgcctc ggagccgcgg aaagttgctg tggagaccag gcccgaagaa ttggtgccca
     1261 agatgtctac gacgacgtca aacagcaaaa tatgacgttg ttcgaggaac atggagtgcg
     1321 tgaactcatc gtcgcttccc cccattgcct gcagaccatc aaccaggact acgcaaaagg
     1381 caccgaactt cacgccaccc attacgccac cttcttccat cgtctggtcc aagacggcca
     1441 ccttatcctt tccaagccca tcgaagccaa agtcacctac cacgacccct gctatctcgg
     1501 gcgccatgcg ggcgaatatg aagcgccccg tgaattgttg cgcgccatcc cggggcttca
     1561 gctcctcgaa atgccacgca atcgggagcg cagcctgtgc tgtggcggcg gaggcggcgg
     1621 catctggctc gacgttccga gcgaggagcg gttctccgtg ttgcgcgtgc aggaagccct
     1681 gcgcacgggc gcggaaatca tcgccaccgc ctgcccctat tgcaccgcca tgctcgaaga
     1741 cgccatcaaa aaccttgatg tcgagcacgc cctgcgggtc ctcgacattg ccgaactcgt
     1801 ggcgcagtcc atgaccaccg aagcgggaca gtaaagacca tgcaagaaag aattggcttt
     1861 tacatctgtc attgcggcat caacatcgct tcccgcgtcc gatgtcccga agtggccgag
     1921 tacgtcgcca ccctgcccga cgtggtggtg tctcgggatt accttttcat gtgctcggat
     1981 cccggccagg aactcatcga aaaggatatc cccgcttacg gtctcacccg cgtcgtggtg
     2041 gcctcctgct ccccccgtat gcacgaaaat acgttccgag gcgcggtcgc ccgcggcggc
     2101 ctcaatccct tccgttgctt tcaccacgtt tgcgttcgag aacacgtctc ctgggttcat
     2161 acggatatgg atgaggccac ggaaaaagcg aaaaccctgg ttcgggcggg cgttcgacgc
     2221 gtggcgcggc aggcagacct gttccccaac accttccccg tcaaccccaa taccctgatc
     2281 gtaggcgggg gaatcgccgg aatgcaggcc gccctcgata tcgcctccgc cggataccac
     2341 gtttatctcg tcgaaaaaca gcccaccatc ggtgggcata tgctccaata cgacaaaacc
     2401 ttccccacct tggattgcgc cgcctgtatc ggtacgccga aaatggtgtc cgtcggacag
     2461 aacaagaata tcgacctgct gacctatgcg gaggtagagg aactaagcgg atttattggt
     2521 aattataccg ctcgggtccg aaaaaaggcc cgctacatcg atgcctccaa atgcaccggc
     2581 tgcggcgaat gcaccaaagt ttgccccgtc gacaaaccca acgaatggga cgtcggaacc
     2641 ctcaagcgcc acgccgtcta ccgctccttt ccccaagccg ttcccatcac cttcgtcatc
     2701 gataaaaacg acagggcccc gtgcgtgcaa acctgccccg cccagaccaa tgcccaaggg
     2761 tatatcgcct tgatcaaagc gggaaaatac ctggaagcca cccaactcat cctcgaaaaa
     2821 cttcctttcc ccggttcgct tggacgagtc tgcccggctc cttgcgaggc cgtatgtcgt
     2881 cgcaagaagg tcgatgaacc tttatctatt cgaaatctaa aaagatttgc ggcagaccag
     2941 gtcgactggg acagtcttcc tctgccccct atcgaaaaaa agagcgaaga tgatcgcgtc
     3001 gctgtcatcg gtagcggccc cgcgggcttg agcgccgcct acttcctcgc gcgaatgggt
     3061 taccccgtat ccgtgttcga agccctcggc gtcgcaggcg gaatgatgcg caccggcatc
     3121 cccgattacc gcctgccacc agccattttg gaacgcgaaa tcaagtatat cgagcgcctc
     3181 ggggtgaact tgcacctcaa cacccccatc ggcaaggaca ggaccgtcga tgacctgttc
     3241 catcaaggct tccgcgccgt atttgtcgct accggtaccc atggcgatgc caaactcggg
     3301 ataccaggcg aggatgccca aggggtcatg gccggcgtgg cctttctgaa acgccaaaac
     3361 ctcgctggcg atgcccgcgt gggcgaagac gtcgtcgtca tcggcggcgg cgcggtcgcc
     3421 atcgatgtgg cacgggtggc gcgtcggatc ggcgccaaac gtgtccgcct gtattgcctg
     3481 gaagcttgcc atgaaatgcc ggcctgggaa gaggaagtcc atgcggcaaa agccgaaggg
     3541 atagaaatcc acaatacgtg gggacccgcg caaatcctgg tttccgaggg caaggcgcgg
     3601 ggcatcgagt tcaaacgttg cacccgcgtg ttcgacgaca aaaagcggtt ttctcccgcg
     3661 tacgacgaag cgcagcgcca agaggtttgc tgcgacaccg tgctcgtggc catcggtcag
     3721 cgtcccgaca cctcgtgggc cgaaggaagc aacgttccct tgcacccccg cggctatgtg
     3781 caggccgatg agcgcacctt cgccaccgaa cgtcctggcc tgttcgccgg cggcgaagtc
     3841 tataccgggc cctcgatcgt cgtgcaggcc gtcgccaacg ggcatgaagc cgccatttcc
     3901 atcgacaggt atcttcgagg agaagacctg ctcgaagcaa gacccgagcg tccgaaaggg
     3961 gagcaatgga acccgattcc cgacgatgcc ttgccgaccc ctcgagcgaa gatgcctgaa
     4021 attgcgttgg atcagcggca ggggttcgag gaggtggaac tcggttttac cgaggcccag
     4081 gccctcgagg aagcctctcg ctgcatcgcc tgtgggacct gctccgaatg catgttgtgc
     4141 gtcgccaact gcaaggcgca ggcgatagac cacagcatga aagacgaagt cttgaacctg
     4201 gacgtgggct cggtcatcgt ggccacgggc ttcgaccccc tcgaccccac gccgatgcac
     4261 gagtacggct atggtaagta ctcgaatgta tatacaaatt tggagtttga acgcttgagc
     4321 aacgcgacgg ggccgacggc gggagcgctg ttgatgcgcg atcccgaaaa ccggtatcat
     4381 tttacgtccc cgccgagcag cgtggccatc ttgcattgta tcggcagccg ggatgtgaat
     4441 catcacgaat actgtagccg cacctgctgc atgtacgcct tgaagtatgc ccatttgcta
     4501 aaggataaag tggggcacga cgttctcgtc tataactttt atattgatat gcgctgtttt
     4561 gggaaaggat acgaggagtt ttatcgtcgg atccaggggg aaggggtgcg catggtgcgt
     4621 ggaaaagcca cgcgtgtgac cgatgaggcg gagagcccgg aggaggaagg caaactggtg
     4681 gtagaagcgg aggatacgct gacaggcaag ctgcttcggg tgccggtgga catggtggtt
     4741 ctttgcacgg cgatggagcc gcgaaaagac acagtagatg ttgcgcgaac atttgggatt
     4801 tcgatagggg gcgatgggtt tttcctggag gagcatccca agttggagcc ggtttcgacc
     4861 gcgacggcgg gtgtgttttt ggcgggtgct tgtcaggggc cgaaggatat tccggacacg
     4921 gtggcgcagg ccaaagcggc cgcgagcatg gcgcaggcgc tcacgtctct tggacgggtc
     4981 gaagtgcagc cgatggtatc gagcatcgat gaagatatct gcgtgggctg caaggtatgt
     5041 attggattgt gtccctattc ggccatcgaa ttcgatgatc gacgggggat atcggtggtc
     5101 aacgatgcag tgtgcaaagg atgcggcagt tgcgccggct attgtccgag cggagcggcg
     5161 aagatacgac attttacgga tcggcagatt ttcggggaaa tcgaaggtct tttggtggca
     5221 aggagcgcgt catgagtgaa aagcagttcg agcccaatat cgttgcgttt ctgtgcaatt
     5281 ggtgcacgtt taccgccgcc gatctcgccg gaacctctcg tctcatctac ccgtcgaacg
     5341 tgaaaatcat ccgcatgatg tgtagcggca tggtcgaccc caagtacgtc ctcaagtcct
     5401 ttctcgaagg ggccgacggt gtattcattg gcggctgctg gccgggggat tgccattata
     5461 tcaacggaaa tatgaaggcg cgtcgacggg tggcgctgat gcatgaggta ttggagcggt
     5521 ttgggatcga acgggatcgg ctgtggctcc gttgggtggc cgcctccgag ggcaatgcgt
     5581 ttgccgatac cgtaacagcg atgacgggcc gactcaagga actcggcccc tgtcgagtga
     5641 gtcgggaagc agtggcttga tcgagcggct aacccacgag cgcggaggct acgacggagt
     5701 gccatgggac agtatacagc gatacagacg gaaggtcata tcaaccaagc gattgccgac
     5761 gtacttggta aacttctgga caagggcgtg gtatcggcgg tgctcgtgcc ttgtcagcaa
     5821 cctcatcgaa aagcggtgat gcagacgctg gtgcgggatc cagaagcgct tgcgcatatc
     5881 gatccctttg caccggtggt gcccaccaat tcggctcggt tggttgcgac actgacgcat
     5941 cacaacgacg cgccggtggc cgcggtagtg cgaagttgcg aagtgcgggc tttcctcgaa
     6001 cttgtcaaac tccatcaagg atgcgcggat aatctgctca tcatcggtat cgattgttat
     6061 ggtcgttatg agaacaagga ttatttcgcc ctggcggccc accaagagga tttgacgact
     6121 cggtttttga tgtcgatggc gagcggagcc ggaggctcgg acggcgtggt tctcggggca
     6181 cctttggcga atgcctgccg gatgtgtgaa taccccgtgg ccgaacccgt cgatatccgc
     6241 ctttgcgcga taggaaccga cgtgacccaa caggtgtggt tcgaatgggt atcggaaaaa
     6301 ggccttcgtg cgatggaaac gctcgcgctg ccttcgcagg aacaaggccc ccaggcaagg
     6361 gaatcagcca ttgccaagct tcggaaggag cggatggccc atcgggatgc cgagttggcc
     6421 gccttccgaa gcaaaactga caccatgacc ggattggcgg acgttgtggc ggcctgtatc
     6481 aactgttata actgccgtgt ggcttgcccg gcctgctatt gcaaggaatg tgtgttcgtg
     6541 acagatacgt ttcggcacga cggagagcag gtgatgcgct gggcgtttca ccgtggtcat
     6601 ctcaaaatgc caaccgatac tgtctttttt catgtcacca ggatgctgca catgagcacc
     6661 ttatgcgtgg gttgtgggca gtgtagcagc gcttgcccga acgatattcc ggtgatggaa
     6721 ctgtttcgga gtgtggcgga gcgcacccag gcccggtttg cctatgttcc ggggtcgctc
     6781 cttacaagaa aaacagccgc tggcggtgtt tcacgctgaa gaattcgatg acgttaccgg
     6841 ccaggtgaaa tagcgaggta cagatggtcg cggtggtcga agataagaca aacctagcgg
     6901 aagagtcccc ttctttcggc tcggtgctca tcgtcggggc tggagtggct ggcattcgcg
     6961 cggccctcga tctcgcccag gttggttatc gggtgtggat gacggatgct tccccagcct
     7021 tggggggcat tttgtccaaa ctcgattatc aatttcccaa cgatcactgc gggatgtgca
     7081 aaatgcttcc cttggtggga cgcgaacaag cctcgcagtt ttgcatgcga aagagccttt
     7141 tccacgataa tatcgagatc ttaccgttca ccgatgtcgt gtcgattcgc ggtgagcccg
     7201 gcgctttcca agtcgacttg ctcgaacacg cgcgctgggt ggatagcgac gtttgcattg
     7261 gcacgggatt gtgcgcttct gtatgcccat tggaagcccc ggaagccttt aaccagggcc
     7321 tgactcgtcg caaagctatc tatcggccgg tcccccataa cttacccaat ctctgggcga
     7381 tcgatgcgga gctttgcgat cgatgcggca aatgcgtcga aatttgcccc gtgggagcca
     7441 tccatctcga tgcccccgac aagcgctcga cccttgccgt ggatgcggtg atcgtggcca
     7501 cgggtacggg gttgtatgac ccttcgcagc agggggatct cagcagctat gggctttccc
     7561 cggacgtggt gacgtccctt caattcgagc ggttgttgag cgggacgggg agatacgatg
     7621 gtgagttgcg tcggccttcg gacgggcggg tggcgaaaaa gatagcgtgg ctgcaatgcg
     7681 tggggtcgag aaatcgcgct caggggcggg attattgctc ttcgatttgt tgtatgtttg
     7741 cgctcaagga ggccgttctg gcgcatgaaa aaggggggga aggtgtcgat accgctattt
     7801 tttacatgga tatgaggacg tttggaaagg attttcagcg atatcggcaa caagcagagc
     7861 aaaagcacgg cgtgagactc atccgctgcc gggttcatac ggtggataaa acgtaggttt
     7921 cgcgcccctt caattcgttg agtggcagtc cagcgcgata att
//
LOCUS       DAOSOR010000240         7892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9174_length_7892_cov_4.148654, whole genome shotgun sequence.
ACCESSION   DAOSOR010000240 DAOSOR010000000
VERSION     DAOSOR010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7892
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9174_length_7892_cov_4.148654"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PK710_13485"
     CDS             complement(<1..383)
                     /locus_tag="PK710_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10784.1"
                     /translation="MVVREQRANATKVERWRRTALAGIIVLSTCLILAFLFSCQRKPT
                     PSLQADSAPSSSASSSAQSWRVWPENEPIIDSHVHIVPTEQGITRALEVFEQVGIGPF
                     VVKSAGFVGSPKYKATLALSQALRGR"
     gene            1063..3825
                     /locus_tag="PK710_13490"
     CDS             1063..3825
                     /locus_tag="PK710_13490"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HOT10785.1"
                     /translation="MAERKSLTGVALGALSNASYADELYEQYRRDPSSVGPEWQTFFQ
                     GFDLASCPRTCVAATQARGQSKVASLIYAYRNQGHLIAKLDPLGNNPTSHPALALETY
                     GLSEEDLPKVFDTGHLGGPQRATLLDILAILKETYCESIGVEYLHIQDVKVRRWLQAQ
                     MEPRRNQPQLSNTAKRQILQNLVDAELFETFIQSRYPGQKRFSLEGAESLIPAVHAIV
                     ELAPDLGVEDFVMGMAHRGRLNVLANILDKSYAQIFSEFEDNFLPESVAGDGDVKYHR
                     GFSSVHVNHAGKPLNISLTSNPSHLEAVNPVVLGRARAKQRRLGDTVIRSRVLPLLIH
                     GDAAFAGQGLVAETLNLSGLPGYTTGGTIHLVVNNQIGFTTGPAEARSTMYSTDVAKM
                     IEVPIFHVNGDDPDAVVYVAELALRFRQEFHRDVVIDMVCYRRHGHNEGDEPAFTQPV
                     LYRKIKDRPSIRRLYQRRLIDDGVLEAVEGEQIADKLKAQLQTAFEEVKRTGGVPEQL
                     AFQDAWEGLDNPYSHTPVETGAAHTVLVDVARALSAIPDGFSLNPKVAKKLPETRKAV
                     EEHGLVDWAGAELLAFGSLLHEGVPVRLSGQDSARGTFSHRHSVWQDMDSQESYIPLR
                     HIHETQARFCVYNSMLSEAAVLGFDYGYSLDEPHMLVLWEAQFGDFANGAQVIIDQFI
                     VSSLSKWKRASGLVMLLPHGYEGQGPEHSNAYLERYLAACAEDNIQVCNFTTPAQYFH
                     ALRRQVRRNFRRPLIVMSPKSLLRNPQAVSPVDDLVTGRFHELLDDPAPPKAPRRLVL
                     CSGKVFYDLANRRKRDGIDDVAIVRVEQFYPYSDDLWNQIESKYDGVQEVLWVQEEPQ
                     NRGGWTFAALHFQRFFPRHSLRYVGREASASPAVGSLRRHQIEQEQLIRDALGLADSH
                     KPVRGA"
     gene            3843..>4289
                     /locus_tag="PK710_13495"
     CDS             3843..>4289
                     /locus_tag="PK710_13495"
                     /inference="COORDINATES: protein motif:HMM:NF004309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HOT10786.1"
                     /translation="MTMIIQVEVPQVGESITEGVLVEWLKRDGDRVVTDDPLFTLETD
                     KVTMTINAEHAGTLKILVPAGQTVKIGQVVAEVDTQPTEASPQAVPQTPGPAFHPIAG
                     APQARNKLAQTDLAELAPSVRRLVQEHELDPKAIEATGRDGRITKGD"
     assembly_gap    4290..4299
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4300..5180
                     /gene="sucB"
                     /locus_tag="PK710_13500"
     CDS             <4300..5180
                     /gene="sucB"
                     /locus_tag="PK710_13500"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF004309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006554 - lysine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HOT10787.1"
                     /translation="EVGQVAVGQIGDVLAHVAREPVPMQQPPQPTPPEVVTTPVQDAP
                     PPVKRLAVLRTTEDIQSRQTRTPMSPLRQRIAERLVQAKNDTALLTTFNEADMSHVIA
                     LRARYKEAFEKKHGVALGFMSFFVKAVVDALQTVPELNAQIQGDEIVQNHFYDIGVAV
                     GTERGLVVPVVRDCDQLSFAGVEKQIAALAKKVRDKTITLPELSGGVFTISNGGIYGS
                     LLSTPILNPPQSGILGMHAIKKRPIAIGDEVVVRPMMYLALSYDHRLVDGREAVTFLK
                     RIVDCIENPERLLLEI"
     gene            5185..6579
                     /gene="lpdA"
                     /locus_tag="PK710_13505"
     CDS             5185..6579
                     /gene="lpdA"
                     /locus_tag="PK710_13505"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOT10788.1"
                     /translation="MDTFDLVVLGAGPGGYVCAIRASQLGLKTAIVEKEPSLGGTCLN
                     VGCIPSKALLESSELLHVARSELAAHGISTGPVAFDLSKMMERKRGIVSQLTDGIGML
                     MKKNKVEVVRGRGVLQGSGNVSVHTSEGVRKLESKAICLAMGSVPVELPFLKFDGTHV
                     VSSTEALSFEAIPKHLVVVGAGAVGLELGSVWSRLGAKVTVIEMLPTITPFADEQAAK
                     TLKKSLEAQGLDIRLETKVKGAVIDGGQVHVSFETKEGNAETIVCDRVLVSVGRKPCV
                     DTAGLAEAGVRLEQGKVWIDERFQTTLPHVYAIGDLVRGPMLAHKASEEGVAVAEIVA
                     GKPATIQYDLIPNVVYTHPELAQVGLTEQQAKQQGIAYKVGKYFYRANGRAKSLGAED
                     GFVKILADRTTDRVIGAHIVGARASELIGELVLGMEFRTAAEDIARTVHAHPSLAEMI
                     KEAALAADRLAIHA"
     gene            complement(6700..7029)
                     /locus_tag="PK710_13510"
     CDS             complement(6700..7029)
                     /locus_tag="PK710_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10789.1"
                     /translation="MNLNRFRIDAPRIPLSPPRTGFSPLRSLPSVDSSDFFELERMAA
                     TLDRSQRFRAESQIDLQPYTLLLNLQLKTARSHRKQLDATFFRSATRLIENIAEVMQP
                     KTRPRSK"
     gene            complement(7110..>7892)
                     /locus_tag="PK710_13515"
     CDS             complement(7110..>7892)
                     /locus_tag="PK710_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10790.1"
                     /translation="QLHFGRLLDRLDCSSAEQLTRRIDVLSNHSETYEAYLGRILDHA
                     YEMPDLLHVKLMDRLDNTFDIHLQHPGITKFNFYRAVFDMLFLPNFGGVTMGRFHFMP
                     DAREGVMLLSQLFKDLIFLTLIRKEKLDTLDSTTRRLFIGLAVAGIREAQWLALEIFN
                     TCITSVPNQREILLEVMQYCTQGGIEGVHLESRGGRLDGMLLSVFAAQIEGKRRELLT
                     ELFEDHERLALMVLTFIVVFASYINDPEYTVRGIGRDGMSAQ"
BASE COUNT         1766 a         2022 c         2384 g         1710 t
ORIGIN      
        1 cgtcctcgaa gggcctgcga cagggcgagg gtagccttat atttcggcga gccgacaaag
       61 ccagcgcttt tgacgacaaa cgggccgatt ccgacttgtt cgaaaacctc caacgctcgc
      121 gtgatgcctt gctcggtggg aacgatatgg acatgggaat cgatgatggg ttcgttctcg
      181 ggccaaaccc gccaagattg tgccgacgaa ctggcggaag aactgggagc cgaatccgct
      241 tgcaggcttg gggttggttt tcgttggcag gaaaacaaaa aagctaaaat caaacaagta
      301 cttaggacaa taattcccgc caaggccgta cgtcgccaac gctccacttt cgttgcattt
      361 gctcgttgtt ctcgaaccac catctcgtgc ccttcatctt gccttgcgct tcaccgtcat
      421 gaaagcattg gcccgacgat gcaagcgttg cgtcgaacgc gcaaaaccat cgtagcacgc
      481 gctccgaccg cctcgcctgc cctttggccc acctcgatgg catccgaaaa cctcaacctc
      541 cacaccaggc gttgaggaac gtcgtaacgg ctttgatgga gcaatcgctg ggaaagccgg
      601 ggaatgcgag agagcgacga acgatagccg ccggatgggt gtgaataacg agattcggtg
      661 tatggctcgg gggcgtcctc ataacgccgg aggaagcgct gggcatgcgt gccaggcatc
      721 ttcaatatca cgaaaattca aggcataacg atggtgtcat ggtctttggc gcaaaggcgg
      781 gcggtgggat acgcagcgag gggtgttgtg ttatcaagtt gggcgttgga ttggcagcgg
      841 gaatcccggg gctccttgga cggttaaaac gcttgtgtcg ataggatggg gcgaggggga
      901 ctggggagat ggaggcgata cgcatgcttg atccgcggaa ggagtcaggt aagctcgggg
      961 ccgttatctt ccggcagctt cttcaaccgg ctttggcatg cgattttccg tgtccttggc
     1021 gcatcgaatt ccggtcgtgt gcaagcaagg agtcatctca tcgtggctga acgcaaatcg
     1081 ttgacgggtg tcgcgcttgg cgcactgtcc aacgcatcgt acgcggatga actgtacgag
     1141 caatatcgtc gagatccatc cagtgtcgga cctgaatggc agacgttttt tcaagggttc
     1201 gacttggcgt cttgtcctcg gacatgcgtt gcggcgacgc aggcgcgggg gcaatcgaag
     1261 gtggcgagcc tcatctatgc ctatcgcaac caagggcatc tcatcgccaa gctcgatcca
     1321 ttgggcaaca acccgacatc gcatcctgcg ctagctctcg agacgtacgg gctatcggaa
     1381 gaggatttgc ccaaagtatt cgatacgggc catcttggtg gcccgcaacg agcgactctt
     1441 ctcgatattc tagcgattct caaagaaacc tactgcgagt ccatcggggt cgagtacctg
     1501 catattcaag atgtgaaagt gcgccgttgg cttcaagcgc agatggagcc gcgtcggaat
     1561 caaccgcagc tttcgaatac ggcaaagcgg cagattctgc agaatctcgt ggatgccgag
     1621 ctattcgaga ccttcattca atcccgttat cctggtcaga agcgattctc cctcgaggga
     1681 gcggagtcgt tgattcccgc ggtgcatgcg atcgtcgaac tcgcgccgga tcttggggtg
     1741 gaggacttcg tgatggggat ggctcatcga ggccgcctca atgtcctggc gaacatcttg
     1801 gacaagtcgt atgcgcaaat cttctcggaa ttcgaggata atttcctgcc cgagagcgtg
     1861 gcgggagacg gtgacgtcaa gtatcatcgg ggattcagct cggttcacgt caaccatgcg
     1921 ggcaagcctt tgaacatcag tttgacgtcg aacccgagcc atctcgaagc cgtcaacccg
     1981 gtggttctgg gtcgagcccg agccaagcag cgaaggcttg gagatacggt gatacgaagc
     2041 cgggtgcttc ccttgttgat tcacggcgat gcggcgtttg cgggtcaggg tttggtggcg
     2101 gaaaccctca atctatccgg gttgcctggg tatacgacgg ggggcacgat ccatctggtc
     2161 gtcaacaatc agattggctt caccaccggt cccgcggaag cacgttcgac catgtattcc
     2221 acggacgtcg ccaagatgat cgaggttccc atctttcacg tgaatgggga tgaccccgat
     2281 gcggtcgtgt atgtggctga gttggcatta cgattccgcc aggaatttca ccgcgacgtg
     2341 gtcatcgaca tggtgtgtta tcgtcgacat ggtcacaacg agggtgacga accggcgttc
     2401 acgcaaccgg tattgtatcg aaaaatcaaa gatcgaccgt cgattcgaag gttgtatcag
     2461 cgaaggctca ttgacgacgg cgtgttggag gcggtcgagg gcgagcagat agcggacaag
     2521 ctcaaggcac aattgcagac ggcgttcgaa gaagtgaagc gaacgggagg ggttcccgag
     2581 cagcttgctt ttcaggatgc gtgggaggga ttggacaatc cttattccca tacgccggtg
     2641 gaaactgggg cagcgcacac ggtattggtg gatgtggctc gcgcgctatc ggcgattccc
     2701 gatgggtttt cgctcaatcc caaagtggcg aaaaagcttc cggaaacgcg caaggcggtg
     2761 gaggagcatg ggttggtgga ttgggccggt gctgaattgc tagcgtttgg gtcgttgctc
     2821 cacgaaggcg tgccggttcg tttgagcggg caagacagtg cacggggaac gttttcgcac
     2881 cggcactccg tttggcagga catggattcg caggaatcct acattcctct tcgccacatt
     2941 catgaaacac aagcgcgatt ttgtgtgtac aacagcatgt tgtccgaagc tgccgtgctt
     3001 ggattcgatt atgggtattc tctcgacgaa ccccatatgt tggtgctttg ggaagcgcaa
     3061 tttggggact tcgccaatgg cgcacaggtc atcatcgacc aattcatcgt gagttcctta
     3121 tcgaagtgga aacgagcctc ggggctcgtg atgcttcttc cgcacggcta cgaaggacag
     3181 gggccggagc attccaacgc gtacttggag cgttatctcg ccgcgtgcgc ggaagacaat
     3241 atccaagtct gcaacttcac cacgcccgcc caatacttcc acgccttgcg tcgacaggtt
     3301 cgtcggaact tccgacgacc gctcatcgtg atgtcgccca agagcctatt gcgaaatccg
     3361 caggccgtat ctcccgtgga cgacctcgta acagggcgct ttcatgagtt gctcgacgat
     3421 cctgctccgc cgaaagcgcc tcgcaggctc gtgctatgca gtggcaaagt cttctatgac
     3481 ctggccaatc gtcggaaacg cgatggaatc gacgatgtag cgattgtccg ggtggagcag
     3541 ttctatccgt attcggatga tttgtggaat cagatcgaga gcaagtacga tggggtccaa
     3601 gaggtgctgt gggtacagga agagccccaa aatcgcggcg ggtggacatt tgcggcattg
     3661 catttccaac gctttttccc tcggcattca ttgcgttacg tgggtaggga ggcgtcggca
     3721 agccctgccg ttggctccct tcgtcgacat cagatcgagc aagagcaact catccgcgat
     3781 gccctggggc ttgcggattc tcataagccg gtgcgcggag cgtagggata ccaggagtcc
     3841 taatgacgat gatcatccag gttgaagtac cccaagttgg agaatcgatc accgaaggcg
     3901 tgttggtcga atggttgaaa cgagacggtg atcgggtggt caccgacgat ccgcttttca
     3961 cgttggaaac ggataaggtc acgatgacca tcaacgccga gcacgcgggg acgctcaaaa
     4021 ttctggtgcc cgccggtcag acggtcaaga tcggacaagt cgttgccgaa gtggacacgc
     4081 aacccacgga agcgtcacca caagcggttc cacaaactcc cggaccggca ttccatccca
     4141 tcgcgggtgc gccccaggcg cgaaataagc tcgcacagac ggacctggcc gaactcgcac
     4201 catccgtacg tcgtctcgta caagaacatg aactagaccc taaggccatc gaagccacgg
     4261 gtcgagacgg ccgtatcacc aagggcgatn nnnnnnnnng tgaagtaggc caggtcgcgg
     4321 tcgggcagat tggcgatgtg ctggcgcatg tcgcgcgtga gcctgtgccc atgcagcaac
     4381 ctccccaacc cacaccgcct gaagtcgtaa caacgcccgt gcaagatgct ccgcccccgg
     4441 tcaaacgtct ggcggtgttg cgaacgacag aagacataca atcgcgtcag acgcgaacac
     4501 cgatgtctcc gttgcgtcaa cggattgccg agcggctcgt gcaagccaag aacgataccg
     4561 cgttgctgac gacgttcaac gaagccgata tgagtcacgt catcgcgctt cgcgcgcgat
     4621 acaaggaagc gttcgaaaaa aagcacggcg tggctctcgg attcatgtcc tttttcgtca
     4681 aagccgtggt ggacgccctg caaaccgtcc cggaattgaa tgcgcagatt cagggcgacg
     4741 aaatcgtgca aaatcatttt tacgacatcg gggtggcggt aggcacggaa cgaggtttgg
     4801 tggtgccggt cgtgcgtgat tgtgatcagc tatctttcgc gggggtggaa aagcagattg
     4861 cggcactggc caaaaaagta agagacaaga ccatcacgtt gcccgaattg tcgggcggcg
     4921 tgttcaccat ctccaacggt ggaatttatg ggagtttgtt gtcgactccg attctcaatc
     4981 ctccccaaag cggaatcctt ggcatgcatg cgatcaagaa acggccgatc gccataggcg
     5041 acgaagtcgt ggtgcgtccg atgatgtacc tggctctttc ctatgaccat cgtttggtgg
     5101 atggacgcga agcggtaacg ttcctcaagc gtatcgtgga ctgcatcgag aatcccgagc
     5161 gccttttgct ggagatctaa cagaatggat actttcgatc tggtagttct tggagctggt
     5221 ccaggcggat atgtctgtgc cattcgtgcg tcgcaactcg ggttgaaaac agcgattgtg
     5281 gagaaggagc cttcgctagg aggcacctgt ttgaacgtcg gctgcattcc ttccaaagca
     5341 ctgctcgaat cgtcggaact gctgcatgtc gcacgttccg aactcgcggc ccatggaatc
     5401 tccacaggtc cagtcgcttt cgatctatcg aaaatgatgg aacgaaaacg cggcatcgtc
     5461 agccaactca ccgatggcat cggtatgttg atgaagaaga acaaggtcga ggtggttcga
     5521 gggcgcgggg tgctgcaagg aagcggaaac gtcagcgtgc atacctccga aggtgtgcga
     5581 aagctcgaat ccaaggcgat ctgtcttgcc atgggcagcg tgcccgtcga attgccattt
     5641 ctgaaattcg atggaaccca cgtggtgtcg agcaccgagg cgttatcgtt cgaggccatt
     5701 cccaagcatt tggttgttgt aggagcaggg gcggtgggtt tggaactggg aagtgtttgg
     5761 tcccggctcg gggccaaggt aaccgtgatc gaaatgcttc cgacgatcac accttttgcg
     5821 gatgaacaag ccgcaaaaac cttgaagaaa tcgctcgaag cccaggggct cgacatacgc
     5881 ctcgaaacca aggtcaaagg agcggttatc gacggcgggc aggtccatgt gtcgttcgaa
     5941 accaaagagg gaaatgccga gacgatcgtc tgcgatcgcg tattggtttc cgtgggacga
     6001 aaaccttgtg tggatacggc gggattggcg gaggcgggcg ttcgtctcga gcagggcaag
     6061 gtatggattg atgagcgatt tcaaacgact ttgccccatg tgtatgccat tggggatttg
     6121 gttcgcggac ccatgctggc ccataaagcg tcggaagaag gagttgccgt ggcagagatc
     6181 gtggcaggca aaccagccac gattcagtac gacctgattc cgaacgtggt gtatacgcat
     6241 ccggaacttg cccaggtggg attgacggag cagcaagcca agcaacaagg cattgcctac
     6301 aaggtcggga agtattttta ccgggcgaat ggacgggcaa aaagtcttgg agcggaggat
     6361 gggtttgtca aaatccttgc cgaccgcacc accgatcgcg tgattggcgc gcatatcgtg
     6421 ggagcgcgag cttcggaact cattggcgag ttggtgctcg gcatggagtt tcgcacggcg
     6481 gcggaagaca tcgcgagaac cgtccatgcg catccatccc tggctgagat gatcaaagaa
     6541 gctgctcttg cggctgaccg gttggccatt cacgcctgat cccgtatctg cccgttttcc
     6601 ccaatcgacc ccgcggcatc taaaaaattc tttcaatttc aatgcgatag agaatttgta
     6661 cccctgcgct gggtatgtac ttcgctcgtc aaagggtcgc tatttcgatc gtgggcgcgt
     6721 ctttggctgc atgacttcgg cgatgttttc tatcaagcgc gtggcacttc gaaagaaggt
     6781 agcatccaat tgttttcgat ggcttcgggc ggttttgagc tgaaggttca acagcaaggt
     6841 gtacggctga aggtcgatct gggattcggc gcgaaaacgc tgggagcgat ccaatgtggc
     6901 ggccattcgt tcaagctcga aaaaatccga tgagtctaca gatggcaagg aacgaagggg
     6961 agaaaaccct gttctaggcg ggctgagcgg tatccgcggg gcgtcgatac gaaaacggtt
     7021 gaggttcacg atgggtcgct ttcttgtcgg aacgttggcg atgcgtaccc aaccgatgag
     7081 ccaccggaaa tgttcccgag acggcggagc tattgtgcgg acatgccatc ccttccgatt
     7141 cctcgcaccg tatattcggg gtcgttgatg taagaggcga acaccacgat gaatgtgagg
     7201 accatcagag cgagtcgttc atggtcttcg aacaattccg tgagcaattc tctgcgtttg
     7261 ccttcgattt gagccgcgaa aacgctgagc aacatcccat cgagccgtcc tccccggctt
     7321 tcgaggtgga ctccttcgat accgccctgg gtgcagtatt gcatgacttc gagcaggatt
     7381 tctcgttggt tcggaacact ggtgatgcag gtattgaaaa tttccagggc aagccattgc
     7441 gcctcgcgga tgccggcgac agcaagcccg atgaacaggc ggcgtgtggt ggagtccaac
     7501 gtatcgagct tttcttttcg gatgagcgta aggaaaatga ggtctttgaa aagctgggat
     7561 agcaacatca cgccttcgcg cgcatcaggc atgaaatgga atcgacccat ggtcacgccg
     7621 ccgaagttgg gcaaaaacag catgtcgaat acggctcgat agaagttgaa cttggtaatc
     7681 ccagggtgtt gcaagtggat atcgaaggtg ttgtccaggc gatccatcag tttgacatga
     7741 agcagatcag gcatttcata agcgtgatcg agaatccttc ccagatacgc ctcataggtc
     7801 tcactatgat tggatagcac gtcgatacgc cgcgtgagct gttcggccga agagcaatcg
     7861 aggcggtcga ggagacgacc gaagtgaagt tg
//
LOCUS       DAOSOR010000241         7883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9188_length_7883_cov_3.688171, whole genome shotgun sequence.
ACCESSION   DAOSOR010000241 DAOSOR010000000
VERSION     DAOSOR010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7883
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9188_length_7883_cov_3.688171"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PK710_13520"
     CDS             complement(<1..371)
                     /locus_tag="PK710_13520"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT10791.1"
                     /translation="MKRHLLFAVFVYFCSFFLGCLSFYPDRPPGTPKMGEFTRVGNEV
                     LRFVDTQPGPPSPAAKDAKKDKGTVVLVHGFGATIEEWVLVIPALKEAGYRVLALDLL
                     GHGWSGRPDADYSILAQARRVG"
     gene            complement(387..1382)
                     /locus_tag="PK710_13525"
     CDS             complement(387..1382)
                     /locus_tag="PK710_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10792.1"
                     /translation="MAISLLVATGCKGEGEDTRECNVHADCASAVCRPDGTCEPITID
                     DAGQETSLDTGPDETDAHEVTDTPEELPPSDGDSPGCKPNGDGTITAAEMPLGPGFNA
                     MFRVSKGVSGFSSEPDCSSGTCLWDLLDLGGVTTDEPSATFALDQKWFAGIKGFENAT
                     YTSRLTDFKLTFGGLSVCDQVQYGVFEVNPSGLFMLGIVSEYAADGTALVYDKPVPLI
                     VFPFHVGTSWTTDTVARGKLCNSIFDYAISQTYTSTVDKLGNVKTPYGTFESVLRINT
                     LMERHLGVGVTATKAVTHTYVTECFGTIAVAVSNEGVSSPEFSGASEVRRLANLP"
     gene            1876..3303
                     /locus_tag="PK710_13530"
     CDS             1876..3303
                     /locus_tag="PK710_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10793.1"
                     /translation="MESTGSASIRAPRTVVANAVDKGISLWYPGVHGTIDTFRTATYT
                     SEHFLDGTVFPAVQAVVGLQEQLAKGFVGTLRGDSTWKQYGKDTWDRFRAGARFRRLV
                     NTMGKELFGSARFAGETVLAENDYFTLTYIPAKEGTAAHIPLFHVGGTIPYGDRLFRL
                     LPEANFYGRFIERGMPVYTMELRGDRYQNNYSSLTLAGLVDSIERMSAVAYRHAGRKM
                     VLEGYCGHGLQALAYLAAKPEDAEEKLCAVAFFVSPIHGPDCATIADFPQRVPASWAK
                     MQTAWLQRTSGYVPGDSMRISLDLALKKNFVKTPMGQFFDGWNQAELAHVRRMEDLSA
                     GQRKMLAGAYWISADNGNRFPIPVDLSRYATAMFVEGISKQGDIPFRYQDKPLSLSGF
                     LSRTSMPVIGFYGGTDPMIPDRTAFILMDLLKERYQHVVHPNAGHISYVFSASQWEKG
                     HKRAFRPNPIDVLLTATGSSEAENR"
     gene            3380..4165
                     /gene="fabG"
                     /locus_tag="PK710_13535"
     CDS             3380..4165
                     /gene="fabG"
                     /locus_tag="PK710_13535"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOT10794.1"
                     /translation="MAIHSGLEDKVVIITGAASGLGRAGALRFAQEGARVAAWDVDEN
                     KAKDLKTAIEQAGGKGLFQFVDVSRSESVNKAVDEVVAAWGRVDVLINNAGIVRDSQL
                     VKWKDGQVAAQMADDAFNRVIDVNLRGVFHCTRAVVPEMIRQGGGAIVSTSSVVGLYG
                     NFGQTNYVAAKAGVIGMTKTWARELGKYKIRANVVAPGFIGTEILTSMPPKVLENMVA
                     HTPLGRIGDPKDIAEAYVWLASDAASFVTGAVLSVDGGLVNGT"
     gene            4299..5888
                     /locus_tag="PK710_13540"
     CDS             4299..5888
                     /locus_tag="PK710_13540"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT10795.1"
                     /translation="MLRGDILGERSRLTPDRTALVYVPTKERISYGRLNERATACARA
                     WIHHFGLRKGDRVAILANNRVEFIEAYFAAIKSGIVLVPLSTKLTASELAFILGDSGA
                     RMLIHASCAASAVRVLEQRMVLEHWVALDEPVVAQDQSYPRMRDAYLRQDLALPRVEP
                     EDLCALLYTSGTTGHPKGVMIPQRMVSWNAYNTVVSWQLRDTDVSPVFMPLYHAGGLG
                     VFVAPLFAIGGTIVLHDGFHAAEILRTVEKERCTVILGVPTVFWFLLRSDAFDTTDWS
                     SVRWLISGGAPLPVKLVEAFRAKGLVLRQGYGLTEVGVNCFSMTNEEATLYAGAVGRP
                     MMFTQARIETQRGEEAETDEVGELMLRGPHVSCGYWDHPEANAEVYDSEGWFRTGDLA
                     TRDRTGMVRIVGRRTDMYISGGVNVFPPEVEAEILRHDAVADVAVVGVPDAIWGETGV
                     AFVLLRPGHRLDKQVLLQSLRQRLASYKIPKTIVFVASLPRTSYGKIRKRELREAYSN
                     GQWEALNPTAAQEEETATAVA"
     gene            5979..>7883
                     /locus_tag="PK710_13545"
     CDS             5979..>7883
                     /locus_tag="PK710_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10796.1"
                     /translation="MASSPALTGSAIRLYLALLLSPRPVSISRCGAFGFADLGTLLKL
                     LSQAVELGWVQEAVRPAGHFQWQDHRHTENLFSQASPEDWKAVFEALDLSSWGLGVAR
                     EAAQSGAWPRARVFYWALVVGLRAHPDLFDKARWLEVVMEALRLFRREVNWLSDEIQA
                     LALEQAVQLGDERAESVLLAIRAYEMLRCGRAGEAMAWCDRALEVSARLDDPETRQHV
                     LLYVANNWMLMGRLREALSMLESFLGDIPPDLLAPSQTIARDTLMEASIATLANLYAW
                     TGKDERAQDLLLRMVEVGKQTRQPLLVKMGSIYLAWYHADRDDLEQARHWSREALAAL
                     EPDDAVTAYCWFASQPAAWVAYRDSDFPLVKDLLERGHHSRVSSGHTHFQNSYLLEVL
                     AELRRQGGSIEGLEFEEELARLTVSPDQRMKGVGLRFRAAQKMESGQGDASEDLRDSM
                     ELLESAGAVPELLRTLRMAASFAARQGNQKEAAALQERLRDVGQFGTTKASAGGSDAR
                     ESHVARAIVDLGRLTQLSHRREGFFGEIVARICRELAVERCALLDSTEEVRVLAARGG
                     DRAWLDSLALRVKGPGGEVVRSVEAGEGGGQLLLIPYRIDELGRKGWICLENHVRPPL
                     LGPGDGPWLES"
BASE COUNT         1592 a         1959 c         2567 g         1765 t
ORIGIN      
        1 ccgacgcgcc gggcctgggc aagaatcgaa taatccgcat ccggacggcc cgaccaacca
       61 tgaccgagca agtccaaggc aagtacacga tatcccgctt ctttcagggc gggaatgacc
      121 aatacccatt cctcgatggt agctccgaat ccgtgaacga gaaccactgt acccttgtcc
      181 ttttttgcgt cttttgcggc aggggacggc gggccgggtt gcgtatcgac gaatcgaagg
      241 acctcattcc ccaccctggt aaactccccc attttggggg ttcccggcgg cctatctgga
      301 taaaacgaca ggcatcccaa aaaaaaggag cagaaataaa cgaaaacggc aaagagtaga
      361 tgtcgtttca tggggacggt tcgctatcag ggaaggttgg cgaggcgacg gacttcggag
      421 gctccgctaa attctgggct tgacactccc tcgttggaca ccgcgaccgc gatggttcca
      481 aagcattccg taacgtaggt atgcgtgacg gctttggtgg cggtgacgcc aacacccaga
      541 tgcctttcca tgagggtgtt gattcgcagc acggactcga aggtgccgta aggcgttttg
      601 acgttgccta gtttgtccac ggtcgaggta tacgtctggc ttatggcgta atcgaatatg
      661 gagttgcaga gctttcctcg ggcgacggtg tcggtggtcc acgaggtacc gacatgaaag
      721 ggaaagacga tcagaggcac gggtttgtcg tagacgaggg cagtgccatc ggcggcgtat
      781 tcggatacga ttccgagcat gaacaggcct gagggattga cctcgaagac tccgtattgc
      841 acctggtcgc agacggacag tcctccgaag gtgagtttga agtcggtcag acggctggta
      901 taggtggcgt tttcgaagcc tttgatgcct gcgaaccatt tttggtcgag cgcaaaggtg
      961 gcggatggtt catctgtggt cacgccgccg aggtcaagca aatcccatag gcacgttccc
     1021 gaactgcaat cgggctcgga ggaaaagccg gatacgcctt tggagacgcg gaacatggcg
     1081 ttgaatcctg ggccgagggg catttccgcg gcggtgatgg tgccatcgcc attgggtttg
     1141 caacctggag aatccccatc gctaggaggt aattcctctg gcgtgtccgt gacttcatgc
     1201 gcatctgtct cgtcaggacc ggtgtcgagc gaggtttctt ggccggcgtc gtcgatagtg
     1261 atgggctcgc aggtgccgtc ggggcggcag acggcggagg cgcaatcggc gtggacgttg
     1321 cattcccgcg tatcttcacc ttcacctttg cagcccgtgg cgacgagaag ggatattgcc
     1381 atggcgcatc ctaacgcgca gaggcgggag gggtcgtgca ggggtcgcgc ggttgaggtc
     1441 atgaacgtcc tgccttggta ggaaacgagc caagtactgg cttttggggc gaaattgtcg
     1501 aatgcggact gtggataagc aatatacggg ccatgctgtt ctttcgtcga tagagtcgat
     1561 ggcacgggag gaagattcgg tgaacctaac gattccgcgg tggtagcgga cgttccccgg
     1621 tggggaaggg gtttttcgaa aagggtcgtt gcctgtgcgc atcgacggta tgttgtcact
     1681 ttaccaaatc ttgtcacgag cgtcacccca gggaagcgat gtaggtgaaa aaaacgggtt
     1741 ccgtttggag gcgcgtgttg gcacggtagg tgcttcggtt gggaacggaa ccgccaagtc
     1801 cgaccactag gggtcgtgaa tcgggggtcc gcttacgctg agcagcgatc tcagcgtctc
     1861 attgaggagt ctacgatgga atcgacagga tcggcttcga ttcgcgctcc gcgcaccgtg
     1921 gttgccaacg cggtcgacaa ggggatatcc ctttggtacc ccggtgttca cggtacgatt
     1981 gatacgtttc gtacggctac gtatacctcg gagcattttc tcgacggtac cgtatttccg
     2041 gccgtacagg ccgtggtagg cttgcaagag cagcttgcca agggctttgt aggtactttg
     2101 cgtggtgata gcacctggaa acaatacgga aaagatacgt gggatcgatt tcgtgcgggt
     2161 gcgcgctttc gccgtctcgt caatacgatg ggaaaagagc tattcggctc cgcacgtttt
     2221 gcgggcgaga cagttcttgc ggaaaacgac tattttacgt tgacctatat cccggcgaaa
     2281 gagggaacgg cggcgcatat accattattt cacgtgggcg gtacgatccc ttacggcgac
     2341 agactctttc gattgcttcc ggaagcgaat ttttacggcc gtttcatcga gcggggaatg
     2401 cccgtctata cgatggaatt gaggggtgac cgctatcaaa acaactattc gtcgctgacg
     2461 cttgccgggc tcgtggactc catcgagcga atgtccgccg tggcgtatcg gcacgcgggg
     2521 cggaagatgg ttctcgaggg ctattgtggg cacgggttgc aagccttggc ctatctggcc
     2581 gccaaaccgg aggatgcgga agaaaaactg tgcgcggtag cctttttcgt atcaccgatt
     2641 cacggaccgg attgcgctac gatcgcggat tttccgcagc gggttcctgc ttcgtgggcg
     2701 aagatgcaaa ccgcgtggct tcagcgaacg agcggctacg tgccgggcga tagcatgcgg
     2761 atttctctgg atttggcgct gaaaaagaac ttcgtcaaaa cgccgatggg gcagttcttc
     2821 gacggttgga atcaagcgga attggcgcat gttcgacgga tggaggactt gagcgcgggc
     2881 cagcgaaaaa tgttggcggg tgcttattgg atctccgcag ataacgggaa tcgatttccc
     2941 attcccgtcg acttgagccg ctatgcgacc gcgatgtttg tcgagggcat atccaaacag
     3001 ggcgatattc cgttccgcta tcaagataag ccattatcgc tgtctggctt cctatcccgt
     3061 acgtccatgc cagtcatcgg attttatggg ggaacggatc caatgattcc cgatcggacg
     3121 gcatttattc tcatggattt gctgaaagag cgataccagc acgtggtgca tccgaatgcc
     3181 gggcatatat cgtatgtatt ctccgcatcc caatgggaaa aagggcataa acgggcgttt
     3241 cgtccgaacc ccatcgatgt cctgctcaca gcgactggaa gctccgaggc ggagaatcga
     3301 tgatggtgaa cgcagggcgg atctaaaaat acaaacaaat caattggtta cgacgttggg
     3361 ggggaggaga taggaaatca tggctatcca tagtggactc gaagataagg ttgtcatcat
     3421 cacgggtgct gccagcggat tgggccgggc cggagcgctg cggtttgcgc aagaaggcgc
     3481 gcgtgtcgcg gcgtgggatg ttgacgaaaa taaggcgaaa gatttgaaaa ccgctatcga
     3541 acaggcgggg ggaaagggac tttttcaatt cgttgacgtc agccgaagcg aatccgtcaa
     3601 caaggctgtc gatgaggtcg ttgccgcctg gggccgtgtc gacgtgttga tcaacaatgc
     3661 aggaatcgtt cgagactccc aacttgtcaa atggaaggac gggcaagtgg ctgctcagat
     3721 ggcggacgat gccttcaacc gtgtcatcga cgtcaatttg cgtggtgtat ttcattgcac
     3781 gcgggcggtg gtgccggaga tgattcgtca aggcgggggt gcgatcgtca gcacatcgtc
     3841 ggtggtgggc ctgtacggca actttgggca aacgaattat gtggccgcca aagcaggtgt
     3901 gattgggatg acgaaaacct gggcgcgtga attgggaaaa tataagatcc gagccaatgt
     3961 ggtggctccg gggttcatcg gaacggagat ccttacctcg atgcctccga aagtccttga
     4021 aaatatggtg gcgcatacgc cgttggggcg catcggcgac cccaaggata tcgcggaagc
     4081 ctatgtttgg ctcgcttccg atgcggcaag ctttgtgacg ggagcggtgc tttcggtcga
     4141 tggaggattg gtcaacggca cgtgagcggt ggggttgtcc atggttggcg gccgctgcga
     4201 tggcaccggc ggagaaggct cgacgacacc cttggaaatc agggacgata gggggtgacc
     4261 tggggtggtg agcgctaggt tgcccaacga ggcatacgat gttgcgtggt gatattcttg
     4321 gggagcgttc tcggttgacg cctgatcgga cagcgctggt ttatgttccc acgaaagagc
     4381 ggatcagcta tgggaggttg aacgagcggg ctaccgcttg tgcccgggcg tggatccatc
     4441 acttcggtct tcgaaaaggc gatcgggtcg ctattctggc caataatcgg gttgaattca
     4501 tcgaagccta tttcgcggcc atcaaatcgg ggatcgtcct cgttcctctg agtaccaagc
     4561 tcacggcttc agagcttgcc ttcatccttg gcgatagcgg cgcaagaatg ctgatccacg
     4621 cctcttgcgc tgcgtccgcc gtacgggtgt tggagcaacg tatggtgtta gagcattggg
     4681 tagctctcga tgagcccgta gtggctcagg accaatcgta tccacgtatg cgcgatgcgt
     4741 atctcaggca agatctggct ctgccgcgcg tggagccaga ggatttatgc gccctactct
     4801 atacgagcgg tacgaccgga caccccaaag gtgtcatgat tccacaacga atggtgtcat
     4861 ggaacgcgta caataccgtc gtgagctggc agttgcggga taccgatgta agtccggtgt
     4921 tcatgcccct ttatcacgcg ggtgggcttg gtgtgttcgt ggctcccctt tttgccattg
     4981 gtggcaccat cgtgctgcac gatggtttcc acgccgctga gatcctgcgc acggtggaaa
     5041 aagaacggtg tacggtcatc cttggggtgc ccacggtttt ctggtttctg cttcgaagcg
     5101 atgctttcga taccacggat tggtcttccg tgcgatggct gatatccggt ggcgcgccct
     5161 tgcccgtcaa gctcgtggaa gcttttcgcg ccaaaggtct cgtcttgcgt caaggctatg
     5221 gcttgacgga agtcggggtc aattgttttt cgatgaccaa tgaagaggca acgttatatg
     5281 ctggcgcggt gggccgtccc atgatgttca cgcaggcgcg aatcgagacg cagcggggcg
     5341 aagaggctga aacggatgag gtgggggagc tgatgctgcg cggaccccat gtttcgtgcg
     5401 ggtattggga tcacccggag gcaaacgcgg aggtttacga ttcggagggc tggtttcgaa
     5461 ccggcgacct ggcaacccga gatcgaacgg ggatggtgcg gattgtggga cgacgcacgg
     5521 atatgtatat ttcggggggc gtcaatgtct ttccaccgga agtcgaagcg gaaatcctgc
     5581 ggcatgatgc ggtggcggat gtggcggtcg tgggggttcc ggatgcgatt tggggggaga
     5641 caggggtagc gtttgtcctt ctgcggcccg ggcatcggct cgataagcag gtgctcttgc
     5701 agagcttgag gcagcgcctc gcgtcgtata agatcccgaa aacaattgta tttgtggctt
     5761 cgttgccacg cacttcctac ggaaaaatcc gaaaacgcga gcttcgcgaa gcgtatagca
     5821 acggccaatg ggaagccctg aacccgacgg ccgcgcaaga ggaggaaacc gccaccgcgg
     5881 tagcgtgata cgaaagggca agaaggcggc ctgacccgct caccccctcg tctcttcgat
     5941 gactttcccg tcggtctgtg gcaggatgcc tcccacccat ggcatccagt cctgccttga
     6001 ccggttcggc catccgcctt tatctggctc tgttgctgag ccctcgtccg gtatcgatct
     6061 cgcgttgcgg tgcgtttggc ttcgcagacc tgggaacgct actcaaactc ctgtctcaag
     6121 ccgtcgaact tggctgggtg caggaagcgg tccgtcccgc gggccatttc caatggcaag
     6181 atcatcgcca tacggaaaat cttttttctc aagcgagtcc cgaggattgg aaagccgttt
     6241 tcgaagcgct cgacctatcg agttggggcc ttggggtagc gcgtgaagcg gcccaatcgg
     6301 gagcctggcc gcgcgcccgt gtgttctatt gggctctggt ggttgggctt cgcgctcacc
     6361 cagacctatt cgacaaggcg cgctggctgg aagtggtgat ggaagccctt cggctttttc
     6421 gtcgggaagt caattggtta tccgatgaaa tccaggccct ggccctcgag caagccgtcc
     6481 aactcggcga tgaacgagcc gaatccgtgt tgctggcgat tcgcgcctat gagatgctcc
     6541 gctgtggtcg ggcaggggag gcgatggcgt ggtgtgatcg tgccttggaa gtctccgccc
     6601 gcctcgacga tccggaaacg cgtcaacacg ttttgctgta tgtggcgaat aattggatgt
     6661 tgatgggacg tttgcgcgag gccctgtcga tgctcgagtc gtttttgggg gatattcctc
     6721 ccgaccttct tgccccatcg cagacgattg cccgcgacac gctgatggaa gcgtcgattg
     6781 cgacgcttgc caatctttat gcgtggacgg ggaaagacga gcgtgcgcag gacttattgc
     6841 ttcgcatggt cgaggtgggt aagcagacgc ggcagccgct gctcgtgaag atgggcagca
     6901 tttatttggc ttggtatcat gcggatcgtg atgatttgga gcaagcgcgc cattggtctc
     6961 gagaagcgct ggcggctttg gagcccgacg atgcggtgac ggcgtattgt tggtttgcct
     7021 cgcagcccgc ggcgtgggtg gcttatcggg attcggattt cccgttggtc aaagacctgc
     7081 tggaacgagg tcaccattct cgcgtgtcct cggggcatac gcactttcag aacagctatt
     7141 tgcttgaggt tctcgccgag ttgcgacgac aaggggggag tatcgaaggg ttggagttcg
     7201 aggaggaact ggcgcgtttg acggtaagcc cggatcagcg catgaagggc gtggggctga
     7261 ggtttcgagc cgcacaaaaa atggagtcgg ggcaagggga tgcctccgaa gacctgcgag
     7321 atagcatgga attgctcgaa tcggcgggag ctgttcctga attgcttcga acgctgcgca
     7381 tggccgcctc cttcgccgca cgccaaggca atcaaaaaga ggctgccgca ttgcaagagc
     7441 ggcttcgtga tgtggggcaa ttcgggacga ccaaagcctc cgcggggggg agtgacgcaa
     7501 gagagagtca cgtagcgcga gcgatcgtgg acctcgggcg ccttacccaa ctttcccatc
     7561 gtcgggaagg cttttttgga gaaattgtgg caagaatctg cagggaactc gcggtcgaac
     7621 ggtgtgcgtt gctcgattct accgaggaag tacgtgtgtt ggcagcgcgg ggaggggatc
     7681 gggcctggct cgattccctc gctttgcggg taaaaggacc tggaggggaa gtggttcgca
     7741 gcgtggaagc tggggaggga gggggccaac tcctgcttat tccttatcga atcgatgaac
     7801 ttggacgtaa aggctggatt tgtttggaaa atcatgttcg gccaccgctg ttggggcctg
     7861 gggatggccc ctggctcgag tcc
//
LOCUS       DAOSOR010000242         7874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9201_length_7874_cov_4.860084, whole genome shotgun sequence.
ACCESSION   DAOSOR010000242 DAOSOR010000000
VERSION     DAOSOR010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7874
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9201_length_7874_cov_4.860084"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1582)
                     /gene="glmU"
                     /locus_tag="PK710_13550"
     CDS             complement(170..1582)
                     /gene="glmU"
                     /locus_tag="PK710_13550"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOT10797.1"
                     /translation="MTELNAIVLAAGHGKRMKSAVPKVMHPVAGRPLVYYPISAALTA
                     GAHRVIVVVGHEREQVMPYLARAFGDRVVIAIQHEQNGTGHATAQALPVLPQRAHRVL
                     ILCGDTPLLAADDLRSLAKTLDSHPDAPLAMLTCTVDDPTGYGRIFRDENGRISEIRE
                     HRDLDNDAQRAIREVNPGVYCANVDFLKEALTQIDSNNAQGELYLTDIVALAAKQGGT
                     IGFAADARSLVGVNDRAQLAATETVMQARIIEKWRRSGVTIRDGAVIHDTVKIAPDAT
                     IENGVTLRGTTTVSAGAVVDVGCVIEDSFLGENTIIRPYTVVSYSRLEAGAHVGPFAH
                     IGAGTMIGTQSQVGNFVETNKTILHPHAKANHLAYIGDAEVGQSATIGAGTIFCNDDG
                     YQKHRTRIGPGAFIGCNSKLVAPVDVGKNAYVATGTTVTQDVPDEALAIGRAQQENKA
                     DYAPTLRHQLKTAARVKHET"
     gene            1769..5665
                     /locus_tag="PK710_13555"
     CDS             1769..5665
                     /locus_tag="PK710_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10798.1"
                     /translation="MRSLSRLFGLMWVPAFCAVSCAGNFDSTRDERPFERHSTLGQEV
                     FGVLCDRVGASVLAEDIEGRSYHALCHPDHNGKYANAVDAFQLPPVSGSAAVVRNLAI
                     AKLERMANRRQDIIRAMDVIAPDIEIDDPYPPKGSTGKRRVRLHKALAELLQRLNPLY
                     DSNPLEAVGGTPEPLFPATTQALARVFASLADNRDAQEALAYMGGRKGYRPAAAALGA
                     IQPVLSYPHLRALSQQSVRMLSPGGPARKQFTQLLNVVHEEMRSSKPSLPLGVLQIDD
                     PEGIAQPNRPRDNLEVLQHVLLATSPRFGTESSQPGLLVMRDYRGFALVTGNTPGVLG
                     SVPAPFADANGDGLADVDAFGRFIGLQGTPVAVDAPFLVPGEPRIRPADPYGRAVLDD
                     GAPAYQYIDTTQTLFSSLMRDMSALLDPDQVNERETMMYALAGATVLFGDRVPSMTYT
                     YGEGKNARTIEYTGFDPDTSPIVDLVHAFGQVLADPQSDDFLAQMIDLFENHENLMAR
                     VVGMALDLKRIADEHPEAGLPRESFFWDHLASVLGKISSAGLDDSDPDAVGLLEDVLL
                     SLTNDDTLALSDVYAKFMTYRDVLNYNRHNINGPAWNYTTNTLNPPKTPVNRALPATG
                     DNMSMFHRSMQIVFDSTRTTSCNKAGAKVYLDAEVIKKFSMNLVYPDDGVFSVLCPST
                     KKDPVDWCDIFQIDDLTQFYLQSIVEDDPKLPASENKGKARLIIKDACLSSLDWATDM
                     DDAFERSSGIEGMSTRPTHQALNRLVFFGADASNLSMPDLDPMRHDEGLLNQKLNQFI
                     WGLQEPMGTPLCAKNSAGVNVCTTAETTLRARNPGALFLWEQFGFAEANRPVLRAFYQ
                     HDREDLFAEMIDVMYFHMPASDHGPECSKTGSWNRDSSSFNPRYCAESGVVTYEPLIV
                     EQLGMDLLPTVHTLLETVAAQKIDSKRYRKALGRGGIQRRGTEVMAAMTRLLFDPDLA
                     AKRKITYRSGTRSTVWNDGQTKMSQTTPFAMLAEAFNRIDDRFALAKDYEPTDRENRQ
                     AQWKKARSQLVDRFLAVEGKGSQARFVNPAIPKAIVMVLRTLREQVNAHCPDREKGVE
                     CTWAKHDMSRKVAEVLADPLFAAIIDLVDELRKDPSRIEIERLAQYLLEAVESDETMR
                     KVLTSAVDLIMVLRDGQTLPPLFNVLSSLSTPESDPAATGSADVTLQLLNVLSRDPDD
                     TTGPDDPLVFDRYRAFDHLVANLVKPIDPSQPSKTALEVLIDVAADIHRADSASDEPL
                     TAEDYRLIADNVRRFLIDDTRGVEQLYEVVRGAKGD"
     gene            5665..7125
                     /locus_tag="PK710_13560"
     CDS             5665..7125
                     /locus_tag="PK710_13560"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOT10799.1"
                     /translation="MIKQAGRMRWAHAAGSAMLLMAMSSGAGAGGLYTTERGVRPMGR
                     GGAFVAGADDLGAVFFNPAGITHASRQVLGDVGFVLMHSKFTRVARVWQTDPNTGQPT
                     GQSWNHTFPTVEGSSQILPVPTVALSYDFGIEGAAFALGVWAPYSASARYEAKVAGVP
                     NPGRYMLLNLDGSALAVPGVWGAYQITSALSVGAGFEMLAGKYRSVSMMNACLPDRWM
                     CAPEAPDYDALTMIDVGPILAPSGNFGIQFDPHPHVRLGASFQLPFYVRAPARSKVRI
                     PSAAVFQDAYQQGDEATVEMDFPWVGRVGVEGRWEKFRGEVAYVYEAWSMHDEIKITP
                     KDIVLRDVLTFPDDYRVTPQTILRNFQDTWSIRLGGETWIELGSYQLDLRAGVMYERS
                     AIPKPYLSTLTIDLDKIIVGLGGGIHVTPTWRIDWMVARTFNTPVDVSPHEAKIEMHT
                     PVRANQPDPSMRDHVNAGHYEANATLLGLGLAVNYL"
     gene            complement(7521..>7874)
                     /locus_tag="PK710_13565"
     CDS             complement(7521..>7874)
                     /locus_tag="PK710_13565"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOT10800.1"
                     /translation="ILPSTHLPGSMAVADRIWREVRGQSFAGETGQPCRVTVSIGVAM
                     YPSPDIKTKESLLRAADTALMTAKREGSDRVCVFQQHGTVYTPDMVPIVRRQEEGPRE
                     RNRHDSNPPSSRSKP"
BASE COUNT         1552 a         2004 c         2539 g         1779 t
ORIGIN      
        1 tgtcggcggt aatatgtagt aaattcaatg tgttactgtt gaaatggagg cctggtggcg
       61 agaggggggc tttcgaaggc cagtcatcga ctcgtggtct tcatggcacg ggtgatgctg
      121 gtggttggga tggtggcgac gggggccgtt aggcttgggg ggaatcgggt taggtttcgt
      181 gtttgacgcg ggcggcggtt ttgagttgat gacggagtgt gggggcgtag tcggctttgt
      241 tttcttgttg ggcgcgaccg atggcgaggg cttcgtcggg gacgtcctgg gtgacggtgg
      301 ttcccgtggc cacgtaggca ttttttccga catcgacggg ggcgacgagt tttgaattgc
      361 agccgatgaa ggcgccgggg ccgatgcggg ttcgatgttt ttggtaacca tcgtcgttgc
      421 agaagatggt accagcgcca atggtggcgg actgtccgac ttctgcgtcg ccgatatagg
      481 ccaggtgatt ggctttggcg tggggatgca ggatggtttt gttggtttcg acgaagttgc
      541 cgacctggga ttgtgtgccg atcatggtgc cggcaccgat atgggcaaac gggccgacgt
      601 gggcgccggc ttcgaggcgt gagtaggaca cgacggtata ggggcgaatg atggtgtttt
      661 cgccgaggaa agaatcttcg atgacgcagc cgacatcgac gacggcacct gcgctgacgg
      721 tggtggtgcc tcgaagggtc acgccgtttt cgatggtggc gtcgggggcg attttgaccg
      781 tgtcatggat cacggcgccg tcgcgaatgg tcactcccga gcgtcgccat ttttcgatga
      841 tccgagcttg catgacggtt tcggtggcgg cgagttgggc gcggtcattg acgccaacca
      901 gcgagcgggc gtcggcggcg aagccgatgg tgccgccttg tttggcggcg agggcgacga
      961 tatcggtgag atacaactcg ccttgtgcgt tgttcgaatc gatttgggtc aaggcttctt
     1021 tgagaaagtc gacgttggcg cagtacacgc cggggttgac ttcacggatg gctcgttggg
     1081 cgtcattgtc gaggtcccga tgttcgcgaa tttcgctgat acgcccattt tcatcgcgaa
     1141 agattcgtcc atagcccgtg ggatcgtcga cggtgcaggt gagcatggcg aggggggcat
     1201 cggggtgtga atcgagtgtt ttggcgagag aacgcaggtc atcggcagcg agcagggggg
     1261 tatcgccgca taggatgagg actcgatggg ctcgttgggg caggacagga agggcttggg
     1321 cggtggcgtg gcccgtaccg ttttgttcat gttggatggc gatgacgacg cggtcgccga
     1381 aggcgcgggc gaggtaaggc atgacttgtt cgcgttcgtg gccgaccacg acgatgacgc
     1441 gatgggctcc tgccgtgagt gccgcggaga tggggtaata gacgagcggt cttcctgcca
     1501 cgggatgcat gaccttgggc acggcgcttt tcatgcgttt cccatgccct gccgccagaa
     1561 cgattgcatt gagttcggtc attcttctgg cactagttcc ggctgctgtc gggtgcaagt
     1621 tgggggtgag gcaaggtcgg gatgcgggag ttttgtgctt cgaaaatggg taagtcgtcc
     1681 gatgaccagg tcccggcgat ttctttctcc ggacatcgtg gaaattgcta cacttaacgg
     1741 tcgacgtccc agcgaaaggg ttgaacgcgt gagaagcttg tcgcgcctat tcgggttgat
     1801 gtgggtgcct gcattctgtg cggtatcctg tgcgggcaac ttcgattcca cgcgcgacga
     1861 gcgtccgttc gaacgccatt ccaccttggg acaagaagtg tttggtgtgc tgtgtgatcg
     1921 cgtgggcgcc agcgttcttg ccgaggacat cgaaggcagg tcgtatcacg cgctttgcca
     1981 tcccgatcac aacggcaagt acgccaacgc ggtggatgcg tttcagcttc cgccagtgtc
     2041 gggctcggcg gcggttgttc gcaacttggc gattgccaag ctcgaacgca tggccaatcg
     2101 acgacaagac atcattcgtg cgatggacgt gattgcgccg gacatcgaga tcgacgatcc
     2161 gtatccgccg aaagggtcga cgggcaagcg gcgtgtgcgg ttgcacaaag ctcttgcgga
     2221 actgctgcaa cgcctaaatc cgctgtacga ttcgaacccg ttggaagcgg tgggtggcac
     2281 acccgagccg cttttcccgg cgaccaccca agctcttgcg agggtatttg cttctcttgc
     2341 ggacaatcga gatgcgcaag aggcgcttgc gtacatggga ggacgaaagg ggtatcgtcc
     2401 ggcggccgcg gcgcttggtg ccattcaacc cgtgttgtcc tatccgcact tacgcgcttt
     2461 gtcgcagcaa tcggtgcgta tgctcagccc cggggggccc gcgcgaaaac agttcacgca
     2521 attgctcaac gtcgttcatg aagaaatgcg ctcgtccaag ccatcgttgc ctttgggcgt
     2581 tttgcaaatc gacgatcccg aaggtattgc gcaacccaat cgtccacggg acaacctgga
     2641 agtgcttcag cacgtgttgc ttgccaccag cccacggttt ggcacggaat cgtcgcaacc
     2701 ggggttgctc gtgatgcggg attatcgcgg ttttgccttg gtgacgggca atacgccggg
     2761 ggttttgggt tctgttcccg cgccgtttgc ggatgcgaat ggcgatgggc ttgcggatgt
     2821 cgatgcgttt gggcgattca ttgggcttca gggcacaccc gtagcggtgg acgctccttt
     2881 tctcgttccg ggagaaccgc ggattcgccc ggcggatcct tatgggcgtg cggtgctcga
     2941 tgatggtgcg ccggcgtacc aatacatcga tacgacccag accctatttt cctcgctgat
     3001 gagggatatg agcgcgctgt tggatcccga tcaggtcaat gagcgtgaga cgatgatgta
     3061 tgcgttggct ggcgccacgg tgttgttcgg cgatcgagtg ccatccatga cgtacacgta
     3121 tggggagggc aagaatgctc gaacgatcga atacacggga ttcgatccgg atacctcgcc
     3181 gatcgtggat ctcgttcatg cgtttgggca agtgttggcg gatccgcaaa gcgatgactt
     3241 tctcgcgcag atgatcgatc tgttcgagaa tcacgagaac ctgatggcgc gtgtggtggg
     3301 catggccctg gacctcaagc gcattgcgga cgagcatccc gaggcgggtt tgcctcggga
     3361 gtcttttttc tgggatcatc tcgcatccgt gcttgggaag atatcgtcgg cgggactcga
     3421 cgacagtgat cccgacgcgg ttggattgct cgaggatgtt cttctttcgc tgacgaatga
     3481 cgacacgctt gcgttatcgg acgtgtatgc gaagttcatg acgtaccggg atgtgctgaa
     3541 ctacaaccgg cacaatatca atgggccggc gtggaactac accacgaata ctctcaatcc
     3601 gcccaaaaca cctgtgaatc gggcacttcc cgcgaccgga gacaacatga gcatgtttca
     3661 tcggagcatg caaatcgtgt tcgactccac gcgcacgacg tcgtgcaaca aggcaggggc
     3721 gaaagtttat ctcgatgcgg aagtcatcaa gaagttttcg atgaacctcg tttatccgga
     3781 cgatggcgtg ttttcggtgc tgtgtccctc gacgaaaaag gatcctgtcg attggtgtga
     3841 tatttttcaa atcgacgatc tgacgcagtt ttatttgcaa agcattgtcg aagacgatcc
     3901 gaagcttccg gcctccgaaa acaaaggaaa ggcgcgcttg atcatcaagg atgcttgtct
     3961 gtccagtctc gattgggcca cggacatgga tgatgccttc gagcggtcca gtgggatcga
     4021 ggggatgtcg acccgtccca cgcaccaggc gctgaatcgt cttgtgtttt tcggagccga
     4081 tgcctcgaac ctatccatgc cggatctcga tcccatgcgg catgacgaag ggttgttgaa
     4141 tcagaagctc aaccaattca tttgggggtt gcaagagccg atggggacgc cgctgtgcgc
     4201 gaaaaactcg gccggggtga acgtttgcac cacggcggag acaacgcttc gtgcgcgcaa
     4261 tccgggagcc ctgtttttat gggagcagtt cggattcgcc gaggcgaatc gtcccgtgtt
     4321 gcgtgccttt taccaacacg atcgggaaga cctcttcgcc gagatgatcg acgtgatgta
     4381 ttttcacatg ccggccagcg atcacggtcc cgagtgttcg aagacaggca gttggaatcg
     4441 cgactcttcg agtttcaatc cgaggtattg cgcggaaagc ggtgtggtga cgtatgagcc
     4501 gctcatcgtg gagcaactcg gcatggatct attgcccacg gtgcatacgt tgctcgagac
     4561 ggtggctgcg cagaagatcg acagcaagcg atatcgaaaa gcgcttggtc gtggtgggat
     4621 ccagcggaga ggaacggagg tgatggcggc catgacgagg ctgcttttcg atccggatct
     4681 tgctgccaag cgcaagatta cttatcgaag tggcacgaga agcacggtat ggaacgatgg
     4741 gcagaccaag atgtcgcaaa ccacaccgtt tgcgatgctt gcggaggctt tcaaccgcat
     4801 cgacgatcgg tttgccttgg cgaaagatta tgagcccacg gatcgagaaa accgtcaggc
     4861 ccagtggaaa aaggcgcgct ctcaattggt ggaccgattc ctcgcggtgg aaggcaaggg
     4921 ctcgcaagct cgattcgtca accctgcgat tcccaaagcg atcgtgatgg tgctgcgcac
     4981 gttgcgtgag caagtcaacg ctcattgtcc ggatcgagaa aaaggggtgg agtgtacgtg
     5041 ggccaagcac gatatgtctc gcaaggtggc ggaggttctt gccgacccgc tatttgcggc
     5101 gatcatcgac cttgtcgatg aactgcgaaa agacccgagt cgaatcgaaa tcgaacgact
     5161 tgcgcaatac ctgctggagg ctgtggaaag cgatgaaacc atgcgcaagg tgctcacctc
     5221 cgcggtcgat ttgatcatgg tgctgcgtga tgggcaaacc ttgccgccgt tgttcaatgt
     5281 gttgtcgtcg ttatcaacgc cagaaagcga tcccgcggcc acgggcagcg cggatgtcac
     5341 gttacaactg ctcaacgtgt tgtctcgcga tcccgatgac acgacgggcc ccgacgatcc
     5401 gttggtgttc gaccgttatc gcgcgtttga tcatctggta gccaacttgg tcaagccgat
     5461 cgatccatcg caaccttcca aaaccgcatt ggaggtgttg atcgatgtgg ctgcggacat
     5521 tcatcgcgcc gatagtgcca gtgatgaacc gttgacggcg gaggattatc gattgatagc
     5581 ggataatgtg cgacgattcc tcatcgatga tacccgcggg gtggagcagc tttacgaagt
     5641 ggttcgcggg gcgaaagggg actgatgatc aagcaagcgg ggcggatgcg atgggctcac
     5701 gccgcgggca gcgccatgct tctgatggcg atgagttcgg gtgcaggggc gggaggattg
     5761 tataccaccg agcgtggtgt gcgtccgatg ggaagaggtg gcgcgtttgt cgcgggagcc
     5821 gatgacctgg gcgcggtatt tttcaatcca gcgggcatca cgcacgcgag ccgtcaggtg
     5881 ctcggcgatg taggattcgt gctgatgcat tcgaagttca cacgcgtggc gcgcgtgtgg
     5941 cagaccgatc ccaacacggg tcagcccacg ggccaatcgt ggaaccatac ctttcctacc
     6001 gtcgaaggtt cctcgcaaat ccttcctgta cctacggttg ccctcagcta cgattttgga
     6061 atcgaagggg cggcttttgc ccttggcgtg tgggcgccct attccgcaag cgcgcgatat
     6121 gaggcgaaag ttgccggtgt ccccaatccc ggccgctata tgttgttgaa tctcgatggc
     6181 tcggcactcg cggtgcccgg cgtatgggga gcctaccaaa tcacaagtgc gctatcggtg
     6241 ggtgccggat tcgaaatgct tgccggtaaa tatcgctccg tatcgatgat gaacgcatgt
     6301 ttgccggacc gatggatgtg cgctccggaa gcacccgatt acgatgccct cacgatgatc
     6361 gatgtgggtc ccatcctggc cccaagcgga aatttcggca ttcaattcga tccccatccc
     6421 catgttcgac taggcgcttc gtttcaactg cctttttacg tgcgcgctcc ggcacgttcc
     6481 aaagttcgaa tccccagcgc cgccgtgttc caagatgcct atcagcaagg cgatgaggct
     6541 acggtcgaga tggactttcc atgggttgga cgggtgggtg tcgaaggtcg gtgggaaaaa
     6601 tttcggggtg aagtggccta cgtgtacgaa gcctggtcca tgcacgatga aatcaagatc
     6661 acaccgaaag acatcgtgct tcgcgatgtg ctgaccttcc ctgatgatta tcgtgtcacg
     6721 ccgcaaacga tcctgcgcaa cttccaggat acctggtcga tccgtctagg aggagaaaca
     6781 tggatcgaat tgggctcgta tcaactcgat ctgcgtgcgg gcgtgatgta cgaaaggtcc
     6841 gcgattccaa agccttatct gtcgaccctc accatcgatt tggacaagat catcgtaggg
     6901 ctcggtgggg gcattcatgt cacgcccacc tggcgaatcg actggatggt ggcccgaaca
     6961 ttcaacacac cggtggatgt ttctccccat gaggcgaaga tcgagatgca tacgcccgtg
     7021 cgggccaatc aaccggaccc gtcgatgcgc gatcatgtga acgcggggca ttatgaagcg
     7081 aacgcgacct tgctcggcct tggtcttgct gtgaactatc tgtgagttcc ccaaagacac
     7141 ttgccgtcag ggcatgagct ttcaagctgc catcaagccc ctacccgctg ctagatcgcc
     7201 atcacgcgag ggtctgacaa ctgggccatg cctggaggga acgacaacgg aacggccatt
     7261 gccgaatcac tcaagccata acatgttgaa attattatat ttacaccgtt ctctccccgg
     7321 tgttgccgca gacatggggc gggtgggggg cgttggctgt acgtgagggg caaaagccga
     7381 tttccttgct tcgcgtagcg agggacaaaa aatactgtgg ggatagatgt tcgataatcg
     7441 ctaataaaat ccaatcgttg caatgactcg ggaagcgagg cttcccggtt ttccctgggg
     7501 gcaatgccga gacaaggact tcacggtttg gagcgggacg agggaggatt ggagtcgtgt
     7561 cggtttcgtt ctcgcggacc ttcttcctgg cgacgaacga taggcaccat atcaggggta
     7621 tagacggttc cgtgctgttg aaagacgcag actcggtcgc ttccttcgcg ctttgcggtc
     7681 atcagagcgg tgtcggccgc acggagcagg gattcttttg ttttgatatc cggagaggga
     7741 tacatggcaa cgccgatcga cactgtgacc cggcagggtt gcccggtttc cccggcgaag
     7801 ctttgcccgc ggacttcccg ccagattcgg tcagcgacgg ccatcgagcc gggcaaatgg
     7861 gtggatggca gaat
//
LOCUS       DAOSOR010000243         7853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9226_length_7853_cov_4.665555, whole genome shotgun sequence.
ACCESSION   DAOSOR010000243 DAOSOR010000000
VERSION     DAOSOR010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7853
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9226_length_7853_cov_4.665555"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1560)
                     /locus_tag="PK710_13570"
     CDS             complement(<1..1560)
                     /locus_tag="PK710_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding EGF-like domain-containing
                     protein"
                     /protein_id="HOT10801.1"
                     /translation="MASDRCPPCGAHGVCDEALGKCGCEAAYGGSQCDRCATGFIRVD
                     DQCVPGMCGDNGDCNDGNPCNGEETCSVDHICVKGDAVDCGPGGACRTSDGRCDCQAG
                     WSGERCDGCAEGYVMREGRCVIAPCETDTDCDDENPCNGKETCNASGECEQGSAMDCG
                     PNGTCEPGSGRCECDLGYEGEDCSRCADGFADFAGQCLRKNCTTDADCSDQKACNGRE
                     VCTSEKVCAPAPAVICGENAVCREPSGSCVCNDGFVWQEQSCKAVSCEMPQAPTLSII
                     HTGAELTFRLSGQEPIEVGATADPKALEPDTWMDGPKWLLTNDGLQRVFARKKGGGCA
                     ADSTFAFTYQVQPKYPPAAGIPGTTAIAKDDPRIVGWATGWVEPVAYGTGVDPAWKTP
                     EKAVGPAQGTSTDIVSLGEGGTITLTFAPPIGNGPGPDFVVFENSFNDTFLELAYVEV
                     SSNGVDFVRFDCAYLAGGSVGGFSGHDSTVMGSLAGSYRQGYGAPFDLDALMMKPEVR
                     SGFIQLTQIRYV"
     gene            complement(1665..2975)
                     /locus_tag="PK710_13575"
     CDS             complement(1665..2975)
                     /locus_tag="PK710_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10802.1"
                     /translation="MLLSKLGSSDRKRSTRFAKAIVVVTSMATGTAIATNACNVESPE
                     GTGGTDVTAGACGRGIIVVNSDYQSTNLSLVAIDGTVLSSSFLSSASTASGLSAPLGG
                     DVVTPTQWQRGDEIVLLDRYPASVLTWVDVRSSTPTGQLSLATGFAANPRDYLLLNPT
                     KAYVTRYQPNLAAGTGNHDEGSDIVVVDPLTRAVTTRLSLRSAMQDAPAVYYPYPDKM
                     LRVGNEVAVLLGAYSSDFMSSASSRIVRIDPQDDRILEVVVLDGMHGCTELALSPSED
                     EIAVSCSGEFAGDSVATVAESGVVLVSAKDEWKERRRFPAAKFGLGPLGSGLAYASDT
                     TVVFTTAGELTEAGDTKREDALLVVDTDSGQFEVALRSSAKPFTFGNVRCASRCAVCF
                     VADASRSAGVVHRYEVNEMGFLGERQEIIVDRTIGLPPRHLGWF"
     gene            complement(2950..5013)
                     /locus_tag="PK710_13580"
     CDS             complement(2950..5013)
                     /locus_tag="PK710_13580"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT10803.1"
                     /translation="MSVVHSSFPPSLLFAFVFAMVTIAHAQAANDDQPRASEKDNDQP
                     VIDVTVRGSRVRSPASSRDPTAASTVISGASLRCTGCATAEVLSQAPGVQITRTGSGA
                     ELATASIRGATSAQTPVLLAGIPINDDVAGTADLSLIPLWMIDRVEVFRGHAPGFAGQ
                     LGLGGAVFFEPRLPSRSGGGLSQTVGSYGELSTAITGSLKAKGAAALIALQHAQARND
                     YPYTDDRGTRFETGDDVRRRRDNADFVMNDAWAVGRMDLGTGADLTIVGNALDREQGV
                     TGLAVVPAHHARASVQRGLTGLTTRVPCAAAKHGEAQGPCLLSLSSAVVTAHTRLSDP
                     LGELSLPSEHVSTKAIRFLQQAQIQHRLGDNHEIGVLGSQAIERLGIDLDDRQGLQAA
                     RYTTRLAASATFALSPDASIHGVGGAECHTTQGSGPSSSCGVFEPYGRAGARWLAAPW
                     LLLLGNAGHYIRPPTLGELYGTSPTVRGTPTLVAENGQNLDAGVRLSKEGQTSTDLAA
                     HLEAFVFSRWVNDLIAYQRTSFGVIRPFNVGSARFVGIEIATRAVWLNHLRHDLTVTL
                     LDPRDTTDGNALTNDILPFQSRMMLSNRFEVFSEPAWSALRLDRAAIALRLQHRSSRF
                     ADPAGLIVIDAHTTFDVEESMDFFDRSLSVRLALRNLFDQPQFDTVGYPLPARSYHAT
                     FEAWF"
     regulatory      complement(5063..5252)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5280..6284)
                     /gene="galT"
                     /locus_tag="PK710_13585"
     CDS             complement(5280..6284)
                     /gene="galT"
                     /locus_tag="PK710_13585"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOT10804.1"
                     /translation="MSELRFNVVTKEWVIIAPERAARPSDHTPPSKPKQPAYRPTCPF
                     CPGNESMAATEVMRLTAPDGSWKTRAIRNLFPILAKAQRAPVHHHVGLQHTVEGFGAH
                     EVIIETPHHDLGLALLEPDQMKAIIETYRDRYNACRQDPHIAHVVLFKNHGAAAGTSL
                     VHPHAQIVGTPVVSYQVRDRIRTLEDHLALYGECVLCRMLAEEIDARSRLLHLSDSFV
                     AFIPYAALSRYHIWIFPRRHIARFGDMTDDEVSDLADTLRTVLRQLHFGLGDPAFNYL
                     VRSAPRSCAQEEFHWYISIVPRIGQAAGFELGSGIFVNTSYPEDSASYLRSVDISEKD
                     "
     gene            7482..>7853
                     /locus_tag="PK710_13590"
     CDS             7482..>7853
                     /locus_tag="PK710_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOT10805.1"
                     /translation="MMNQYCDLHELGWSEWFAQRADCRSPDTVARVAAVDRDQLVLVD
                     QDGSFRAKLAGRYLHHHHLNQEMPCVGDWVCVGKQSGDDFGVIHALLERRTSLRRKSA
                     GTTTEYQMIAANVDYVLIVQSC"
BASE COUNT         1706 a         2335 c         2244 g         1568 t
ORIGIN      
        1 cacataacga atctgcgtga gttggatgaa gccggaacgc acttccggtt tcatcatcaa
       61 tgcgtcgaga tcgaagggcg cgccgtatcc ttgccgatag cttcccgcta gcgaacccat
      121 gacggtcgaa tcgtgaccgg agaagcctcc gacggagcca ccggcaagat aggcgcaatc
      181 gaatcgcacg aagtcgacgc cattgctgct tacttccaca tacgccagtt cgaggaaggt
      241 atcgttgaag gaattctcga aaaccacaaa gtctggcccg ggaccattgc cgatcggggg
      301 ggcgaacgtc agcgtgatgg ttcctccttc cccaagcgac acgatgtctg tcgaggttcc
      361 ctgcgcgggc ccgacggctt tttcaggtgt tttccatgcc ggatcgacac cggtcccata
      421 cgcgacaggt tccacccagc ccgtcgccca tcccacgatg cgggggtcgt ctttggcgat
      481 ggccgtcgtt cctggaatcc ccgcggcggg agggtatttc ggctgcacct ggtaggtgaa
      541 agcaaaggtc gaatccgcgg cacaaccgcc gccctttttc cttgcgaata cccgctgcaa
      601 cccatcgttc gtcaacaacc acttcggacc atccatccag gtgtccggct ccagggcctt
      661 cgggtcagcc gttgctccca cctcgatggg ttcttgaccg gaaaggcgga aagtcaattc
      721 cgcgccggta tgaatgatcg atagcgtggg tgcttgcggc atttcgcagg acacggcttt
      781 gcaagattgt tcctgccata cgaagccatc gttacaaacg cagcttcccg agggctccct
      841 acacacggcg ttttccccac aaatcacggc gggggcagga gcgcatacct tctccgacgt
      901 gcatacttca cgtccgttgc acgccttctg atctgagcaa tccgcatccg ttgtgcagtt
      961 ttttcgaaga cattgcccag cgaagtccgc gaatccgtcc gcacacctgc tgcaatcctc
     1021 cccttcatat ccaaggtcac attcacaccg tcccgaacct ggctcgcacg tgccgttggg
     1081 tccgcagtcc atcgcgctgc cttgctcaca ttcgcccgag gcgttgcagg tttcttttcc
     1141 gttgcacggg ttctcatcat cgcaatccgt atcggtttcg cacggagcga tcacacaacg
     1201 cccttcgcgc atgacgtatc cttccgcgca accatcacat cgctccccac tccaaccggc
     1261 ctgacaatcg caacgtccat cactcgttcg acaggccccc cccgggccgc agtccacggc
     1321 atcccctttc acgcaaatat ggtccacact acaggtttcc tcaccgttac aagggtttcc
     1381 atcgttgcaa tcgccattgt cgccgcacat acctggcacg cattggtcat ccacgcgaat
     1441 gaaacctgtc gcgcaccgat cacattggct tcccccatac gccgcctcac acccacattt
     1501 tcccagcgcc tcgtcacaga caccatgcgc tccacaagga gggcaacgat cggacgccat
     1561 ataagaatcc gagcaggata ttccaagcgt cacccaacac atcacgcaaa aaccaatgca
     1621 atctcgtgcc ttcatcgatg ccatcgcttt tctttcatcg ctcatcagaa ccaccccaga
     1681 tggcgaggag gaaggccaat cgttcgatcc acgatgattt cctggcgctc acctaaaaac
     1741 cccatttcat tcacctcata gcgatgcacg acccctgccg atcgtgacgc atcggccaca
     1801 aaacacaccg cgcatcgcga tgcacagcgg acattgccga acgtaaatgg cttagccgac
     1861 gatcgaagcg ccacttcgaa ctgtccggaa tcggtatcca cgacgagcag agcgtcttct
     1921 cgtttcgtat ctcccgcttc ggtcaattcc cccgcggtgg tgaatacgac cgtagtatcg
     1981 gaggcatacg ccagcccgct tcccaacggt ccgaggccga atttcgcggc gggaaaccgc
     2041 ctccgctcct tccattcgtc ttttgccgat accagcacga caccggattc cgccacggta
     2101 gccaccgaat cacctgcaaa ctctcccgag caagacacgg cgatttcatc ctccgaggga
     2161 gacaaagcca attccgtaca tccgtgcatc ccgtcgagca cgacgacctc gagaattcga
     2221 tcgtcttggg gatcgatgcg aacgatccga gaggatgccg acgacatgaa gtcggaagaa
     2281 taggctccca gtagcacggc cacctcattt cccacccgca gcatcttatc cggatacgga
     2341 tagtacacgg ctggcgcgtc ttgcatggcg gagcgcagcg ataggcgtgt cgtcacggct
     2401 cgcgttagcg gatcgacgac aacgatgtcg ctcccttcgt cgtgattgcc ggtccctgca
     2461 gccaggttcg gttgatagcg agtcacatag gctttggtgg gattgagaag cagataatcc
     2521 cgcggattgg cagcaaatcc ggtagccaaa gacaattgtc ctgttggcgt tgaggaacgc
     2581 acatccaccc aggtaagaac ggatgcggga taacgatcga gcagaacgat ctcatcgcct
     2641 cgttgccatt gtgtgggtgt tacgacgtct ccgccaagcg gcgcggacaa ccccgacgcc
     2701 gtggaagcag aagataggaa agaggaggac aataccgtgc catcgatggc caccagcgac
     2761 aggttggtcg attgataatc ggaattgacg acgatgatgc cgcggccaca agcacctgcg
     2821 gtaacgtccg ttccccccgt tccctcgggg gactccacat tgcatgcatt ggtagcgatc
     2881 gcggtgcccg tggccatcga cgtgacgacc acgatggcct ttgcgaatcg cgtcgaacgc
     2941 ttgcgatcgc tagaaccaag cttcgaaagt agcatggtag ctccttgcgg gaagcggata
     3001 accaaccgtg tcgaattgcg gctggtcgaa aaggtttcga agtgccaggc gaacggaaag
     3061 ggagcgatcg aaaaagtcca tcgactcttc tacatcgaag gtcgtatgcg catcgatgac
     3121 gatcaacccc gcgggatctg caaaacgcga cgaacgatgc tgtaggcgaa gggcgatggc
     3181 agcacggtcg aggcgaaggg ccgaccaggc gggctcactg aacacctcga atcgattgga
     3241 gagcatcatt cgcgattgaa acggcaggat atcgttagtc aaagcattgc cgtcggtcgt
     3301 atctctaggg tcgagcaagg tgactgtcag gtcatgtctg agatggttga gccatacggc
     3361 tcgggtggct atttcgatcc ccacgaagcg ggcggaaccc acgttgaacg gacgaatcac
     3421 cccgaagctc gtgcgttggt acgcgatcaa gtcgtttacc catcgggaaa acacgaaggc
     3481 ttcgagatga gcggcgagat cggtgctcgt ctggccctct ttcgacagac gtacgcctgc
     3541 atccaggttt tggccgtttt ccgcgacgag cgtaggtgtg ccgcgaactg tgggggacgt
     3601 gccatagagt tccccaagag tcggaggcct tatgtagtga ccggcgttgc cgagcagcag
     3661 caaccaggga gcggcgagcc agcgcgcgcc cgccctgcca tagggttcga aaacgccgca
     3721 ggacgacgat ggtccagagc cttgcgtggt atggcattcc gctccaccga caccgtgaat
     3781 cgacgcatca ggggaaagcg caaaggttgc ggaagccgcc aaccgcgtgg tgtaacgcgc
     3841 cgcttgcaag ccctgtcgat cgtccaaatc aatgccgagc cgttcgatgg cttgtgaacc
     3901 caatactccg atttcatggt tgtcgcccaa acgatgctgg atttgagctt gctgaaggaa
     3961 gcgaatcgct ttggtagaca cgtgttccga agggagagac agctctccta aaggatcgct
     4021 gagccgcgtg tgagcggtaa cgaccgcgct cgataaggac agcaggcacg gcccctgggc
     4081 ttccccatgt ttggcggcgg cgcaaggaac gcgcgtggtg aggccggtca atccacgctg
     4141 aacggatgcc cgtgcatgat gagcggggac cacggcaagc cccgtgactc cttgctcacg
     4201 gtccagggcg tttcccacga tggtcagatc ggctccggta cccagatcca tcctgcccac
     4261 ggcccaggca tcgttcatca cgaagtcggc gttgtcgcga cgccgtcgta catcatcgcc
     4321 ggtttcgaat cgggttcccc tgtcatccgt atacggatag tcattgcgcg cttgcgcatg
     4381 ctgcaaagcg atcagggcag cagctccctt tgctttcaga ctgccggtga tggccgtcga
     4441 aagttcaccg tagctaccga ccgtttgcga tagtcctccg ccagaacggg atggcaagcg
     4501 cggctcgaag aatacggcgc ctcccaggcc aagctgtccg gcaaaccctg gagcatggcc
     4561 tcggaacacc tcgacgcgat cgatcatcca taaagggata agcgacaaat ccgccgtgcc
     4621 cgcgacgtca tcgttgatcg gaatccctgc caacaacacg ggagtttgcg cgctcgtcgc
     4681 cccgcgaatc gaagccgtag ccaactccgc gcccgacccc gtgcgagtaa tctgaacgcc
     4741 tggcgcctgg ctcaatacct ccgcagtagc gcaacccgta caacgcaaag aggcgccgga
     4801 tatcaccgtc gatgccgccg tgggatcacg ggaagaagca ggagatcgaa cgcgcgagcc
     4861 acgaaccgtc acgtcgatga ccggctggtc gttgtccttt tcggaggcgc ggggttggtc
     4921 gtcgttggca gcctgcgcat gcgcgatggt taccattgcg aaaacgaagg caaacagcag
     4981 cgagggcgga aaagaggagt gaacgaccga catgaatcca cttctggagc gagcgcgcgc
     5041 tccgcaaggg ggtgaccgtt tcaaaaacgg tgtgtcgtcg aacaggtctt ccgacttccc
     5101 gctgcctcac gtcgccttcc cagcttgcgc cagtggcttc atgacgtgag ggataacgtg
     5161 gttacggcgg cggctccgtg ccggatttgc accggcttcc ctgggtggtt ggaccacccc
     5221 cccgcgaagg ggttcgaaga cagcggcatc caagcacacg cgggcgctag gtgcaagcat
     5281 cagtcttttt cggagatgtc cacggagcgc aaatacgacg cgctgtcttc cggataggat
     5341 gtattgacga aaatccccga tcccaattcg aatcccgccg cttgcccaat tcgcggcacg
     5401 atcgagatat accaatggaa ttcctcttgc gcgcaggagc ggggcgccga acgaaccaga
     5461 taattgaatg ccggatcgcc gagcccaaaa tgaagctgcc gcaataccgt gcgcagggta
     5521 tccgccaggt ccgatacttc gtcgtccgtc atgtcaccaa accgcgcgat atgacgtcgg
     5581 gggaagatcc aaatatggta acgcgacaag gcggcgtagg gaatgaacgc cacaaacgaa
     5641 tcggaaaggt gcaaaagacg cgaacgggcg tcgatctcct ctgcgagcat tcgacacaac
     5701 acgcattccc cgtacaaggc gagatggtct tccaaggtgc ggatacgatc tcgcacttga
     5761 taggaaacca cgggcgtacc cacgatctgc gcgtgcggat gcaccaagga agttcccgcg
     5821 gcggcaccgt gatttttgaa gagcaccacg tgagcgatat gcggatcttg gcgacaagcg
     5881 ttatagcgat ctcgataggt ttcgatgatg gctttcatct gatcgggctc gagcaacgcg
     5941 agccccaggt cgtgatgcgg tgtttcgatg atgacttcgt gagcgccaaa gccttcgacc
     6001 gtgtgttgca atcccacgtg atgatggaca ggcgcgcgct gtgctttggc gaggatggga
     6061 aatagatttc ggatggctcg ggttttccaa gaaccatcag gggccgtgag acgcatgacc
     6121 tcggtggcgg ccatggattc gtttccaggg caaaacgggc atgtgggacg ataggcgggc
     6181 tgtttgggtt tcgagggggg ggtgtgatcg cttgggcgtg cagcacgttc cggcgcaatg
     6241 atgacccatt ccttggtgac gacgttgaaa cgtaattccg acatgttccc cgtggtgagt
     6301 atagtgcgtg acgtggagcg gagagaagac gtttgtcgct cgcgcatccc cttggctgtc
     6361 aagcatggca ggggcaaacg atacgtttgg ggatagcaag cccgtcggta ggtcgtcctc
     6421 gagtcgatcg attcattcca cggggcccat aggcgtactg tcgggcactt tctcgaccac
     6481 gacgcgggca agatcacatc caccgtgagt tttgccttca aacatgtaca ccatggcagg
     6541 ataggacatg tcgacaacgg gagcgttgca ctttattttt acggaattgc agttggagcc
     6601 tcgagccaac gtcaccaacg cgaccgatct ttcgttgtga caatcggtgg cgcgaatgaa
     6661 ccctccacaa ccgtatcgat ccgcatcgac cgcgaaccac ttcaggtttg ccttacattc
     6721 cgggccgctt tgcccactgg ggcaatgccc cttgggtccg caagcaaact cgcaactacc
     6781 atacgggtcc ttgtaaatgg accgattgct tttccgataa cacccaaacc aacttgtcga
     6841 aatagcccat tggccggaat ctcattgggc gtgctctcgt tggcgctatt acgaacgctt
     6901 ggtggctgca agggcttcga gtcgaatcca tcgcacaacg gcggaggtgc cacttcgatc
     6961 tgtatcgtat gcgtcgccac gggagcagcg tcttgtaagt atccgtatag ggataggcaa
     7021 cgttgaccaa gttccgaaaa cgaatacgtg atgtcatgcg acccttcctg ggtcggatcc
     7081 catgagagcg gtatttcatg aacgccgtcg gcaatgagtg ccacccggat gacattggcc
     7141 gcctctaacg aaaaagaaac cggattgtgt accgtgggcc atcgacaggc gaagtgatgg
     7201 ccacataagg tgtgggtgtt tcaccgccct ggccgacagc tcctgcctgt cgcgaccatc
     7261 ccgcggtgcc agcgccctgg cccgatgcac cggcgttggc cccggcagtt cctgctgctg
     7321 gcattcggcc agaagcgata cttcaccaag gtcaccgcat agctgacgtt ggcgaccttg
     7381 ctattccatt gggttcgcca caaaatgctg cgatgagcgg gactcgcgac accctcgtta
     7441 cgcgccgtag catcgatgac attcaatgat cgaaacagaa tatgatgaat cagtactgtg
     7501 acttgcatga gctgggctgg agcgagtggt ttgcgcaacg ggcggactgc agatcgccgg
     7561 ataccgttgc ccgcgtagcg gcggtcgacc gcgaccaatt ggtgctcgtg gatcaggacg
     7621 ggagttttcg ggcaaagttg gccggccgtt acttgcacca tcaccacttg aaccaagaga
     7681 tgccgtgcgt aggcgactgg gtttgcgtgg gaaagcagtc tggcgacgat tttggggtaa
     7741 tccacgcgct actggagcgt aggacttctc tacgcaggaa atcggctggc accaccactg
     7801 agtaccagat gattgcagcg aacgtggatt acgtgctcat cgtgcagtct tgc
//
LOCUS       DAOSOR010000244         7775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9355_length_7775_cov_4.341192, whole genome shotgun sequence.
ACCESSION   DAOSOR010000244 DAOSOR010000000
VERSION     DAOSOR010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7775
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9355_length_7775_cov_4.341192"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PK710_13595"
     CDS             <1..445
                     /locus_tag="PK710_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10806.1"
                     /translation="EFLADQVEKLSEGFRFLPSPRHLVPYVVETLLYWGIAAAGMWLL
                     AWGCGIEDMTFVRSCALLGVLGLGIIVPGAPGYFGAFQGSIYAGLALYFVESQVLGPG
                     AAFVFILYVTQLGVMFLMALVAVLLDRNSVAATPLSVTTSGDRLA"
     gene            429..977
                     /locus_tag="PK710_13600"
     CDS             429..977
                     /locus_tag="PK710_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10807.1"
                     /translation="MTVWHDNQGKLAHYDRACILYAMMTRGQMLGTWAAMALAGLATL
                     CWITADAQAKASYTCPYSLTQTYSAALRLIRVDNGFAVTERDPDAAYLMFEYESRESG
                     SRVTPGAIEMVPNGEGVMVVVKLPKMPRYHEEVLLDALKRKLQSDYGEPPSKQKPKAD
                     ERPDGGTDAAPDGSARKGKPHR"
     gene            complement(1163..1239)
                     /locus_tag="PK710_13605"
     tRNA            complement(1163..1239)
                     /locus_tag="PK710_13605"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1203..1205),aa:Asp,seq:gtc)
     gene            complement(1320..2507)
                     /locus_tag="PK710_13610"
     CDS             complement(1320..2507)
                     /locus_tag="PK710_13610"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOT10808.1"
                     /translation="MGIVGRHVGWLFGAFTMFGVAMIACSGTDGGSADFGSGAGAHKG
                     GSGGSSGKGGAGGSGGSNAIDGGEDVIVFDTGFTGDREIDPDSSCAELTLQAEAVPLD
                     MYFMVDVSGSMSGSNITALKNGLTNFFNDAQSAGLGAAAQRFPIAKYNDPYDETCDQN
                     AYAKPAVPWAQLPNAPLVSWINSLNASGYTPTIPALNGSVQACHDRLTTMPTHKCAVV
                     LVTDGMPEGGCPPTSSAAEAPLGQVAAKAWADGIPVFAIGFPGLNYVGEAVLNTIAAQ
                     GGTGTPVIISGGNVGQAFIDALEDIRGTALGCEYQMPKAEAGTVNPTLVKVTYKAGTA
                     SQPDTVPRVDSAKDCATGDGWYYDDNTSPSKLIMCPSTCDKMQNDEGGEVQILLGCSS
                     NQR"
     gene            complement(2614..4293)
                     /gene="ettA"
                     /locus_tag="PK710_13615"
     CDS             complement(2614..4293)
                     /gene="ettA"
                     /locus_tag="PK710_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOT10809.1"
                     /translation="MSVEFVFTTVGLRKVTSTGKEVLRAMSLSFLPGAKIGVIGPNGS
                     GKSTLLRIMAGQDKDYFGEAFAGKGVTIGYLPQEPELDPSLDVRGNVELGVKETRDLL
                     VRFEQLSTKLGEPLDDDEMNDVMDEFGRVQEAIDHAKGWDLDRNLEIAMDALRLPAGD
                     ASVQTLSGGERRRVALCRVLLERPQMLLLDEPTNHLDAESVAWLERHLQEYEGTVIAV
                     THDRYFLDHVAEWILELDRGFGRPYKGNYSGWLEQKQTRLAQEEKQESSRQRTLQREL
                     EWIRMAPRARQAKGKARLNAYEKLLSEDHGERVAQGEIVIPPGERLGDKVIVAEGLRK
                     GYGDRLLIDDLSFIIPKGAVVGIIGANGAGKTTLFRLIVGQERPDAGTLHLGDTVQLA
                     YVDQSRAQLDPSHSIWEEISGGQDTVRLGKRDVNSRAYVGSFNFKGADQQKPVGLLSG
                     GERNRVHLAKLLRSGGNVLLLDEPTNDLDVDTLRSLEEAILSFAGCVLVISHDRWFLD
                     RIATHIMAFEGDSNVVMLEGSYQDYEKDLLARRGVDAVDPHRIKFRKLIRD"
     gene            4645..4914
                     /locus_tag="PK710_13620"
     CDS             4645..4914
                     /locus_tag="PK710_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10810.1"
                     /translation="MTPQKLAPYILRTLARHQQEGRGANLDTLVQELGVRRADVRQTV
                     SALHREGYVDALRMRLTLLGFAIGTALSPMNLPPLRTRRGIVAAA"
     gene            5094..6338
                     /locus_tag="PK710_13625"
     CDS             5094..6338
                     /locus_tag="PK710_13625"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT10811.1"
                     /translation="MGDSESLPVEVGRYQIEKVLGQGAMGRVLLAHDPVLDRAVAIKI
                     LRTDLDISAEMRDGLLRRMRNEARAAARVVHPNIVTLHDMIEDPSLGLCLVFEFVSGE
                     TLKDRVSKGPSPALEMARVAREIGSALSLAHDAGVLHRDIKPENIMLAPYGAKVADFG
                     IARVPDSTLTRTGGLLGTPAYSAPETISGSNFSPLSDQFSLAATLYEAISGLRAFPGD
                     DAVTVAARIGTEEPKPIAQSLGLDPRVDQVLGRALSKDPLRRFATCAAFGEALAAALE
                     IGTMLDSHKVPETLAPTRAIGLSASVGLGGAAVGALIAVLLYSLMRPPSPTPFDIVAA
                     RASGSSSTAPALASTDTTSRARTKPRPVDTDPSWVPTIAYRDAGSDGHADRDASKSID
                     DSGPISDANQEAAKSPKERDGG"
     gene            6702..7322
                     /locus_tag="PK710_13630"
     CDS             6702..7322
                     /locus_tag="PK710_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10812.1"
                     /translation="MKTMRWLAIVTLVAGASLIACGDDEDDNGKNSAGSGGATGGTGG
                     GVTGGTAGEQTGGTGGEETGGSGGGVTGGSGGGVTGGTGGGETGGTGGGETGGTGGGA
                     GAAGSAGQGGGGGEINPCVLKCISDHPAEWKALVDLMAPCVCEKGVCDDVCETSFCAD
                     PIDYEPPADCASCALAKGESTCQAEVVQCLGDSNCSSILTCLLACK"
     gene            7549..>7775
                     /locus_tag="PK710_13635"
     CDS             7549..>7775
                     /locus_tag="PK710_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10813.1"
                     /translation="MKISHWLIVASFIAPVFACSSSDDNKKSNTNPGEGGSGGSGGGV
                     LVGGSGGGSNTGGGNTGGSNTGGSNTGGSNTG"
BASE COUNT         1626 a         2273 c         2168 g         1708 t
ORIGIN      
        1 tgagttcctt gccgaccagg tggaaaaact atccgaagga tttcgattcc ttccatcacc
       61 acgacacctc gttccttacg ttgtcgaaac gctgctgtat tgggggatag ccgccgcggg
      121 aatgtggctg ctggcatggg gttgcggaat cgaagacatg acgttcgttc gttcgtgcgc
      181 gctactcggt gtgctcggct tgggaatcat cgttcctggg gctcccggtt acttcggcgc
      241 gtttcaaggc tccatttacg cgggtcttgc gctgtatttc gtggaatctc aggtcctcgg
      301 tcccggagca gctttcgtct tcatcctcta cgtcacacag cttggcgtga tgtttctgat
      361 ggccctcgtg gctgtgttgc tcgaccgaaa ttccgtagcc gcaacgcccc tgtcggtgac
      421 gacttccggt gaccgtctgg catgacaatc aagggaagtt ggcccattac gacagggcgt
      481 gcatcctgta cgcgatgatg actcgcggac aaatgctcgg aacgtgggct gcgatggcac
      541 ttgcagggct agccacgctt tgctggataa ccgcggatgc gcaagccaaa gcttcttaca
      601 cctgtcccta ttcgctgaca caaacctata gcgctgcctt gcggctgatt cgcgtggaca
      661 acggttttgc agtcacggaa agggatcccg acgccgcgta tctcatgttc gaatacgaga
      721 gtcgcgaaag tggctcgcgc gtcactccgg gcgccattga gatggttcct aacggcgaag
      781 gagtcatggt ggtggtcaag cttccgaaga tgccccgcta tcacgaagag gttctcctcg
      841 acgctctcaa gcgcaagttg cagtccgact acggggaacc accatccaaa caaaagccaa
      901 aagccgatga gcgaccggat ggtggaacgg atgccgcacc ggatggttcg gcgcgcaaag
      961 gcaaaccaca tcggtaaaaa ataatgcgtc gaccacgcag ccacacatcg atcggccttc
     1021 ggtcggtgcc gtgcttccag cgttcggcga atcttgcgta acttaatgaa ttaattgtta
     1081 ttttcgcatt tccgaagatg gcgtcctcgt ttgcccatcg aggccacaaa acgcgaaaag
     1141 ctctgccttg gcagagcttt tctggcgggg cggacgggac ttgaacccgc ggcctccggc
     1201 gtgacaggcc ggcgttataa ccaacttaac taccgcccca atgcggtact gtcgaagtgg
     1261 gtcggggtta tggtcggagt tgatctgttt gtcaagccca aagcgatgaa tcgatttggt
     1321 taacgttggt tgctggaaca accgagcaga atctgcactt ctcccccctc atcattttgc
     1381 attttgtcac aagtcgaagg gcacatgatc aacttcgaag gggacgtgtt gtcgtcgtaa
     1441 taccaaccgt ctcctgtcgc gcaatccttc gcgctatcca cgcgaggaac tgtatccggc
     1501 tggctcgcgg ttcctgcctt gtacgtgact ttcaccaggg tcggattcac ggtccccgcc
     1561 tcggctttgg gcatctgata ctcacaccct aacgccgtac cacgaatgtc ttccaacgca
     1621 tcgatgaaag cctgccccac gttaccccct gaaatgatca cgggggtgcc ggttcctccc
     1681 tgtgccgcaa tcgtattaag tacagcctct ccgacataat tcaatccggg gaaaccgatc
     1741 gcaaacaccg ggatcccgtc agcccatgct ttcgccgcca cttgtcccaa cggtgcctcc
     1801 gctgccgaac tcgtgggcgg gcaccccccc tccggcatcc cgtccgtcac caataccacc
     1861 gcacatttat gcgtgggcat ggtggtcaag cgatcatggc aagcttgcac agagccattg
     1921 agcgcgggaa tggttggcgt gtagccgctg gcgttcaggc tgttgatcca actcaccagc
     1981 ggtgcattcg gaagctgagc ccaaggtacc gctggcttcg catacgcgtt ctgatcgcaa
     2041 gtttcgtcgt acgggtcgtt gtacttggca atgggaaatc gttgtgctgc cgcgcccaag
     2101 ccggccgatt gcgcgtcatt gaagaagttg gtgagaccgt ttttcagggc ggtgatgttc
     2161 gatccactca tggacccgga cacgtcgacc atgaaataca tgtccaaagg aacggcttcg
     2221 gcttgcaacg tcagttccgc gcacgacgaa tccggatcga tttcccgatc tcccgtgaag
     2281 cccgtgtcga aaacgatcac gtcttcccca ccatcaatcg cattggatcc gcccgagccg
     2341 cctgctcctc ccttccccga cgacccgcca gagccgcctt tatgggcccc cgctccggac
     2401 ccgaaatccg cgcttccgcc atcggtacca ctgcacgcga tcatggcgac gccaaacatc
     2461 gtgaatgcac cgaacagcca acccacgtga cgaccaacga tacccatatc gtttctcccg
     2521 aaacaaagtg tgcgatggat gaaagatagc agattgaacg aggcagaacc acgacaacgc
     2581 gccagcagcg tcattatcct accggaacat gaatcaatcg cggatgagtt tgcgaaactt
     2641 gattcgatgc gggtcgaccg catccacacc ccgtctcgct agtaggtctt tttcgtagtc
     2701 ctggtagctt ccctcgagca tcaccacatt cgagtctccc tcgaacgcca tgatatgggt
     2761 ggcgattcgg tcgaggaacc aacggtcatg gctgatgacc agcacacaac ccgcgaatga
     2821 taggatggcc tcttccaacg agcgaagcgt atcgacatcc agatcgttgg tgggttcatc
     2881 gagcagcagc acgttgccac cgcttcgaag cagtttggcg agatggacgc ggttgcgttc
     2941 tcccccggac agcaacccca ctggtttttg ctggtctgcc cctttgaagt tgaagcttcc
     3001 cacgtaggcg cgggaattga cgtcgcgctt tcctaggcgg acagtgtcct gaccgcccga
     3061 aatctcctcc caaatcgaat ggctggggtc gagctgggcc cgagactggt cgacatacgc
     3121 aagctgcacg gtatcgccga gatgcagggt tccggcatcg ggtcgttcct gtcccacgat
     3181 caaacgaaat agcgtggttt tgcccgcgcc attggctccg atgatcccca cgactgcccc
     3241 tttggggata atgaatgaca agtcatcgat gagcagacga tcgccgtatc cttttcgtaa
     3301 cccttcggcc acgatgacct tgtcgccgag ccgttcacca gggggaatga cgatctcacc
     3361 ttgcgcaacc cgttccccat gatcttccga aagcagcttt tcataggcgt tcaagcgcgc
     3421 ttttcctttg gcttggcgcg cacggggagc catgcgaatc cattcgagtt ctcgttgtaa
     3481 cgtccgttga cgggaagact cttgcttctc ttcttgcgca agccgcgtct gcttctgctc
     3541 caaccaaccg gaataattac ccttatacgg acgaccaaag ccacgatcga gttccagaat
     3601 ccattccgcc acgtgatcca aaaaataccg atcgtgcgtc acggcaatca cggtcccctc
     3661 atactcttgc aaatgccgtt cgagccaagc caccgactct gcatccaaat ggttcgtggg
     3721 ctcgtcgagc agcaacatct gtggcctttc aagcaacaca cggcataagg caacacgcct
     3781 ccgctctccc cccgacagcg tctgcacgct tgcatctccc gcgggcaaac gcaacgcatc
     3841 catcgcgatc tccaagttgc gatcgagatc ccagcccttg gcatgatcga tcgcttcctg
     3901 caccctgcca aactcgtcca tcacatcgtt catctcatcg tcatcgagag gctcgccgag
     3961 cttcgtgctc aactgctcga aacgaacgag cagatcccga gtctccttga cgcccagttc
     4021 gacgttgcct cgaacgtcga gacttggatc gagttcgggc tcttggggca gatacccgat
     4081 ggtcacgcct tttccagcaa aggcctctcc gaaataatcc ttgtcttgcc ctgccatgat
     4141 ccgcagcaag gtgctttttc ccgatccatt gggtccgatg actccaattt tcgcaccggg
     4201 aagaaaggac aaggacatgg cgcgaagcac ttccttgccc gtggaagtca cttttcgcaa
     4261 acccaccgtg gtgaaaacga attcgaccga catgcgccct tccttgtcgt taccgtcccc
     4321 ggctgtcaat tgacttcgcg gtcacaccaa aaaaccgcaa tcccgcttcc cctaggaaaa
     4381 gcagcggcgc aagggatgtc gtttttttcg caactcggcc gactggctgt ccgatggcga
     4441 gacatgcact ttcgaaaaat ggttgggaat agaaaagccc tcgaatcgtt ggttccccaa
     4501 ggtgcccctc acggcatccc tgcaccgtgt tggggcttgg gccataaggg gtttcatgaa
     4561 aacaccgccg cccttggcag gggccccgat caatcttttg tcaaaaactg tacaaatgtt
     4621 cagagagact ttaaggctgc tcccatgact cctcaaaaac tggctcccta catcctgcgc
     4681 acgcttgctc gacaccaaca agagggtcga ggggccaacc tggacacctt ggtgcaagaa
     4741 ctcggcgtac gtcgagcgga tgtgcgtcaa accgtatccg cattgcatcg agaaggttat
     4801 gtcgatgccc ttcgcatgcg tttgaccttg cttggttttg cgatcggcac ggcactatcc
     4861 cccatgaatc ttcctccttt gagaacgcgt cggggcatcg tggcggcggc atgattcaaa
     4921 aaatcgtgcc accatcgtca tgaataacga attcattgaa ccgcgagttt ttgggctggc
     4981 cgagcaaaaa gaccgcgaaa ggaaggaaag ggcttggttg acggacttgg ttccgccatg
     5041 cttgatcgcg ctcggtggtg tcctttaagg gttcgctatc gacttttatg ctcatgggtg
     5101 attcggaaag cctacctgta gaggttggac gctatcagat cgaaaaggtc ctcggccagg
     5161 gtgccatggg acgcgttttg ctcgcgcacg atccggttct cgatcgagcc gtagccatca
     5221 agattttgcg tacggacctc gatatcagcg cggagatgcg cgacggattg ctgcgacgga
     5281 tgcgcaacga agcgcgggca gccgcacgag tcgtgcatcc gaacatcgtc acgctgcatg
     5341 atatgatcga ggacccgagc cttgggcttt gtctcgtttt cgaattcgtg tcgggagaga
     5401 ccttgaaaga tcgtgtctcg aaaggtcctt cgccggctct ggaaatggcg cgagtggctc
     5461 gtgaaatagg atctgccttg tctctcgctc acgacgcggg cgtgttgcat cgtgatatca
     5521 aaccggaaaa catcatgctt gccccttatg gggcgaaagt cgcggacttc gggattgccc
     5581 gagttccgga ttccacgttg acgcgcacgg gaggattgtt gggcacgcct gcctacagcg
     5641 ctccagaaac gatcagcgga agcaacttca gcccgttgag tgatcaattc tcgcttgcgg
     5701 caacgttata cgaggcgatc tccgggcttc gagcatttcc gggagatgat gcggtcacgg
     5761 tcgcggctcg catcggaacg gaagaaccca agccgatcgc ccaatcattg gggcttgatc
     5821 cgcgagtcga ccaagtgctc ggacgtgccc tcagcaagga cccgctgcgg cgtttcgcca
     5881 cgtgtgccgc attcggggaa gcgttggcag cggccctcga aatcggcacc atgctcgaca
     5941 gtcacaaggt tcccgaaacg cttgcaccca ctcgcgccat aggtctttcg gcaagcgtcg
     6001 gacttggtgg agcggccgtc ggcgccctca tcgcggttct tctctattcc ctgatgcgcc
     6061 ctccaagccc aacccctttc gatatcgtgg ccgcaagagc ctccggctca tcgtcgactg
     6121 cccccgccct tgcctccacc gatacaacct ctcgtgcccg aaccaaacct cgtcccgtgg
     6181 atacggaccc ctcgtgggta cccaccattg cctatcgcga cgccggctcc gatgggcatg
     6241 cagacaggga tgcaagcaag tccatcgacg attccgggcc catttcggat gcgaatcaag
     6301 aggcggcgaa atccccaaaa gagcgagacg gcggttgacg gcggtgcagt cgcgtagtct
     6361 tcaagaacgg gcgtgccgtg ggcgaaattc ccaaaagagc gagacggcgg ttgacggggc
     6421 acttacgcgt ctatttcgaa gcacgggcag cccctcgctg cacggcatgg atcaggtgag
     6481 aggaaaagga gtcagaaaag catgacttct acccaccgtt ggattcccct gcttccgctt
     6541 atcgaagata agcatttgta atttatgggt attttgtttt ttcaaaccca ttcttgccct
     6601 gatgcccatt ttcctcgtac cttgactcat atctcggctg ggtcatactc caatatccag
     6661 tctttcgtgg cgaatgccac tcgtcttgga gggatataac gatgaaaaca atgcgttggt
     6721 tagcgatcgt gactttggta gccggagctt ctctgattgc ttgtggtgac gacgaggacg
     6781 ataatggcaa gaactccgcg ggttcgggcg gagcgactgg agggacgggt ggaggagtga
     6841 cgggcggaac tgctggtgaa caaactggag ggacgggtgg tgaagagacg ggcggaagcg
     6901 gtggtggagt gacgggcgga agcggtggtg gagtgacggg cggaaccggt ggtggagaga
     6961 cgggcggtac tggtggtgga gagacgggcg gaaccggtgg tggtgcgggt gcagcgggta
     7021 gcgccggcca gggcggtggt ggaggcgaga tcaacccctg cgtattgaaa tgcatttctg
     7081 atcacccggc tgaatggaaa gctttggtcg atctgatggc tccgtgtgtt tgcgaaaaag
     7141 gggtctgtga cgacgtctgt gaaacttctt tctgtgcgga tcccattgat tatgagcctc
     7201 cagccgactg cgcgagctgc gcgctggcga aaggcgagag cacctgtcag gccgaagttg
     7261 ttcaatgcct cggcgacagc aattgttcca gcatactgac gtgtctattg gcctgcaaat
     7321 agtgtagttc ttccctcctg tggcccgatt cccccgtgcc gaacaaccca tcgactgtcg
     7381 cgccatcggg cgcggaaaaa aacgcgggtg cccccttgga gcgatcctga ttcacagttt
     7441 ttccccataa gaacaattct tttgccgcgc gcgcttgaca gagggccttt cacacgatac
     7501 gccaaacgaa gctcgatcgc gggcaggtgt accttaggag gccctctcat gaagatttct
     7561 cattggctca tcgtcgctag ctttatcgct cccgtctttg cttgctcctc atcggacgac
     7621 aacaaaaaat ccaatacaaa tcctggcgag ggaggaagtg gcggctccgg tggtggcgtc
     7681 cttgttggtg gctccggcgg cggcagcaac accggcggcg gcaacactgg tggcagcaac
     7741 actggtggca gcaacactgg tggcagcaac actgg
//
LOCUS       DAOSOR010000245         7775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9356_length_7775_cov_4.041969, whole genome shotgun sequence.
ACCESSION   DAOSOR010000245 DAOSOR010000000
VERSION     DAOSOR010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7775
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9356_length_7775_cov_4.041969"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="PK710_13640"
     CDS             <1..735
                     /locus_tag="PK710_13640"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOT10814.1"
                     /translation="RDHQLVDITRLGLNQIEISADAVRIGATVTLAELVAAAQIPATA
                     GEVLRKVARGIASAPLRNMITVGGGIAHIVYWADMPVALLALDATVEVQRAKEKPVQI
                     PIDFCLRDGKKTWDGGLITSVRVPLDSDKISFGYERFTRTASDYAFASACVTLRNVGG
                     VAKRVRVVLGAVQGRPSRLASVEALVEGQAITPQLLETIEKNVHSSVQVAPNFRAPAE
                     YRRELAAVLVGRALDSAWAQASREEG"
     gene            735..1220
                     /locus_tag="PK710_13645"
     CDS             735..1220
                     /locus_tag="PK710_13645"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOT10815.1"
                     /translation="MRISTRINGVARQFDCQPGDTLFDVLRRMGLRGVKEGCDREGTC
                     GACTVLLDGKAVLSCITPAPKVAGREVLTIEGLGDVASPHPIQVAFADAGAVQCGYCS
                     PGMILSTKSLLDRTLVPTQEQIAEALSGNLCRCTGYVKIFDAVRDAAAALQQEKKDNE
                     R"
     gene            1210..3540
                     /locus_tag="PK710_13650"
     CDS             1210..3540
                     /locus_tag="PK710_13650"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOT10816.1"
                     /translation="MSADRTRDLQVVGHSVRKVDSLGLACGQELYVADYATPDTLVGY
                     VVPSPYAHARIKRIDATRARAIAGVHAVLTHEDIPRIAHTTAGQGYVEPSPYDNFVLD
                     SKVRFVGDRVALIAAETLEIAEEAGKALDIEWEMLDPIFDPDHAMDEGAPIIHDEPEA
                     HMPIPVPYDPKRNMAAGANMGDGDIEQALRTSPKVYSRDFSTQYAQHCPIEPHVTLGY
                     LDARNRVVLITSTQVPFHARRITAQAVGIPVKRVRVIKPRIGGGFGAKQEVLLEPLVA
                     SLVLATGRKILMQLSRAEEFLSRTRHAIKVGIDGGFNEDGTINGLRMRVQSNTGAYGA
                     HALTVMCNCGSKVLPLYRMKAVAFDAKAVYTNLPVGGAYRGYGATQAAFAMECFLDEV
                     AEDLHLDPIEIRRRNHILPGEGSPVFAALGEGKPGVEQKIGSSGLAECLTLGAKAIGW
                     EEKRSKPGTGTLRRGIGMCALMQGSAIPEIDLGAVHIKMNDDGSFNLTCGATDLGTGS
                     DTILAQIAAEVLGVDETEVLVYSSDTDLTPFDVGAYASSTTYLSGEASRRAALAVAEQ
                     IREIASEMMESHPDPATLVLKDKAVVAPDGSRVTLGEVGLRSLYQANQRQVAATGSAI
                     SHKSPPPFSAHFVEVTVDIQTGQVRVVKYVAAVDCGTAINPKLAEGQVEGSVLNGISY
                     ALTEEYQFDDKGRMRNTGFNDYKIFGTQDTPEIQTILVPTWEETGPYGAKSVSEICIN
                     GALPAISNAIYDAVGVRLREGPFTPDRVLAAIRQKA"
     gene            3800..5782
                     /locus_tag="PK710_13655"
     CDS             3800..5782
                     /locus_tag="PK710_13655"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOT10817.1"
                     /translation="MALLGLAIALYGCGSCAATPPTQLFPMSKRPYRIPGYSVCLFAT
                     LVGCGSDPSEQSSSPPPVVDKPPEAVPLLLVDRAPSLLPSAGPVEGAWAAFADLDGDG
                     RPDIAQPTSQGLLLYWNGESKFEKASASALPSEFTAPKKPAEDEEPIVFQQVLAGDFD
                     ADGLSDLLIVSTGKTPLRLLTHKDARSFQSQTIAVPEGSILGHAAVADIDGDGDTDVI
                     VTLGGEASGSTGAPNALLLINDGKGKFADQSLARLVAPGLSSRGVAVGDVDGDGAPDL
                     VFSGDTTGHRLLLNDGKGYFRDAPPDALPAGQAPGGKIPAMGDLNGDGALDILIPSSQ
                     ANQVLINDGKGNFTDETAFVLGSQPGKANVAVLVDLDRDTLLDVVLAGPSVPLRILRN
                     DGTGRLFDYSANMVPQGPAASEVVSLGVADVDGDKDPDLFVSRQGLASPWLLVNWHPE
                     PMDDTDGDGIPDEIDNCPKEPNPDQANSDAYAFNCSTARQCKTATGCVLKVFGESSYL
                     LCDEPKTWQAARAFCRSRGADLVVIDSKEENELLAASGIPTAWIGLSDQQTEGSFLWV
                     NGKSLTTAYWNDDEPNDSGGAEDCVGMFTSGETAGLWNDFSCDAEHAFICEEKTFRGP
                     QDPGDACDVCPNVYDPNQKDSDADGVGDACTTESPE"
     gene            5791..>7775
                     /locus_tag="PK710_13660"
     CDS             5791..>7775
                     /locus_tag="PK710_13660"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOT10818.1"
                     /translation="MQRWMLVAGLVVLPTGGLLAACGDTEQVAEITVISVEPAEGSAR
                     GGERLILRGSGFDASARVRFGEAAATEVRFISSSQLEVVTPLHLAGAVDITVQAAGAE
                     ATLPRGFAFLPLELSFREAPAWYLPTFEDTPTDVLAQDFDGDGHPDLLLSRPNRPALF
                     LPNSGTGSFAGATVVPQTPDAGVDADAGQAEGGDGGDFEGGTDAQPAEAGASDAGDST
                     GAALAKTWTRHTARMLSTDIDGDGDLDVILCNRGGQPHALAKNIGKGEFSIVEGAFPE
                     TSDECRDAVLTDVDGDGLEDVVVVGAGKVGGGKSYLRVYRRVSSGDSVSFVASNNTEK
                     EDKLAETSCATVTGTAEVEVSSKLSRTTAAQGNVSCRIGFETAADTGSLEAWFALPKL
                     PVLLDAITFSARLETGSQALRVLVRDANGEVFAWDAGTVDATGWKSIRASKLGSWQAW
                     EGGDGVMDLPLDAVGVSLSLSSAATSALFLDALALEVPAIGKVYIDDFERKEFTHAWT
                     DMRTQISVGDLDGDGLGDLLIASAAQGAQSPLVLLRNASVAGEIAFRPVLSGALEALT
                     EPVAATALLDVDEDGDLDIVVGVVAGQDRYFSNDGKGYLFDDTLAMMPVDRVDAVSMT
                     VVDLDLDGRQDLLIANDRAVDRVYLGRGASGFRDATP"
BASE COUNT         1517 a         2109 c         2492 g         1657 t
ORIGIN      
        1 cgagatcacc agctcgtcga catcacacga ctcggtctga accagatcga gatctctgcc
       61 gatgccgtgc gaatcggagc cacggtcacc ctggcggaac tcgtcgccgc tgcccaaatc
      121 cccgccacgg cgggtgaggt tttgcgaaag gtcgcacggg ggatcgcctc cgctcctttg
      181 cgcaacatga tcacggtcgg cggaggcatc gcccacatcg tgtattgggc cgatatgccc
      241 gtcgcgctgc tagctctcga tgccacggtc gaggtgcagc gcgccaaaga aaaacccgtt
      301 caaatcccca tcgatttctg tttgcgcgac ggaaaaaaga cctgggatgg cggattgatc
      361 acctccgtgc gcgtgccgct ggactccgac aagatttcgt ttggctacga acgctttacg
      421 agaacggctt cggattatgc gtttgcatcc gcgtgcgtga cgctacggaa cgtcggtggt
      481 gtagcgaagc gagtgcgagt ggtgctgggt gcggtgcagg ggcgtccatc gcgcctggca
      541 tctgtcgaag cccttgtgga aggacaggcc attacgccac aactactgga aaccattgag
      601 aaaaacgttc attccagcgt tcaggtcgcg cccaacttcc gcgcgccagc ggagtatcgt
      661 cgcgagcttg ccgcggtgtt ggtgggaagg gcgctcgatt cggcctgggc tcaggcgtcg
      721 cgggaggaag ggtgatgagg atatcgactc gaatcaacgg tgtggctcga caattcgact
      781 gccagcctgg cgataccctt ttcgatgtat tgcgtcgcat gggattgcga ggggtcaagg
      841 aaggctgtga ccgcgagggg acttgtggcg cgtgtacggt gctgttggat ggcaaggcgg
      901 tattgagttg catcacgccg gctcccaagg tggctggccg tgaggtattg acgatcgagg
      961 ggttgggaga cgtggcttct cctcatccga tccaggttgc ctttgcggac gcgggggcgg
     1021 tgcaatgcgg gtattgctcg ccgggcatga tcctatccac caagtcgctg ctcgaccgga
     1081 cgttggtgcc gacccaggag cagatcgccg aggcgctttc gggcaatctt tgccgatgca
     1141 cggggtatgt gaagattttc gacgccgttc gcgatgcggc cgcggccttg caacaggaga
     1201 agaaagacaa tgagcgctga tcgcaccaga gacttgcagg ttgtcggcca ttccgttcgc
     1261 aaggtcgatt cccttgggtt ggcgtgcggt caagagctgt acgttgccga ctacgccacg
     1321 ccggacaccc tggttgggta cgtggtgccc agcccttatg cccacgcccg catcaagcgc
     1381 atcgacgcca cccgtgcgcg cgcgatcgcg ggcgttcacg cggtgcttac ccacgaggat
     1441 atccctcgta ttgcccatac gacggctgga caggggtatg tggagccttc gccttatgac
     1501 aacttcgtgc tcgacagcaa agtgcgattc gtgggcgatc gggtggccct gattgccgcg
     1561 gaaaccctcg aaattgctga agaagctggg aaagccctgg acatcgaatg ggagatgctc
     1621 gaccccattt tcgatcccga ccatgcgatg gacgagggcg cgcccatcat tcacgatgag
     1681 ccggaagccc atatgcccat tccggttcct tatgacccca agcgaaacat ggccgcaggt
     1741 gccaatatgg gagacgggga tatcgaacag gcccttcgca cctcccccaa ggtttattcc
     1801 cgggatttca gcacgcaata tgcccagcac tgccccatcg agccgcatgt gaccctgggt
     1861 tacctcgatg ctcgcaatcg tgtcgtgttg atcacctcga cgcaggtccc gttccacgcg
     1921 cgacgcatca ccgctcaggc tgtcggcatc ccggtcaagc gggtgcgggt gatcaagccg
     1981 cgcattggcg gcggatttgg cgccaagcaa gaagttttgc tcgagccttt ggtggcgagt
     2041 ctcgttcttg ccacgggccg caagattctg atgcaactca gccgggccga ggaattcctt
     2101 tctcgaaccc gtcatgccat caaggtgggt atcgacggtg gtttcaacga ggatggcacc
     2161 atcaacggcc ttcggatgcg tgtgcagtcc aacaccggcg cctatggtgc ccacgcgctt
     2221 acggtgatgt gcaattgcgg ctccaaagtc ctgcccttgt accggatgaa ggcggtggcc
     2281 ttcgacgcca aagcggtata caccaacctg cccgtgggcg gagcctatcg cggttatggt
     2341 gccacccagg cggctttcgc gatggaatgc ttcctcgacg aggtcgcgga ggatctgcac
     2401 ctcgatccga tcgaaattcg ccgtcgaaat catattttac ccggtgaagg atctccggtt
     2461 ttcgccgccc ttggagaagg caaacccggt gtcgagcaaa aaattggttc ctcggggctg
     2521 gccgaatgct tgacccttgg ggccaaggcg atcggttggg aagaaaaacg cagcaagcct
     2581 ggaacgggaa ccttgcgacg gggcattggc atgtgcgcgt tgatgcaagg ctccgcgatc
     2641 ccggaaatcg acctgggagc cgttcatatc aagatgaacg atgacggttc gttcaacttg
     2701 acgtgtggag ccacggactt gggaacgggc tccgacacga ttttggccca aattgccgcc
     2761 gaggtgctcg gagtcgatga aaccgaggtg ctggtatatt cctccgatac ggatctgact
     2821 ccgttcgatg taggggctta tgcctcgagc accacctact tgtcgggaga ggcgtcgcgt
     2881 cgcgcggccc tggcggtagc cgagcaaata cgcgaaattg cttcagaaat gatggaatcc
     2941 caccctgatc ccgccacctt ggtgttgaaa gacaaagcgg tcgtggcgcc ggatggttcc
     3001 cgggtaacgc tcggtgaggt aggattgcgt tcgctctacc aggccaatca gcgtcaggta
     3061 gccgccaccg gctcggcgat ttcgcataaa tcaccgcccc ccttctctgc ccatttcgtg
     3121 gaggtgacgg tcgatatcca gactggacaa gtgcgtgtgg tcaagtatgt agccgcggtg
     3181 gattgcggca cggccatcaa ccccaagctg gcggaaggac aggtggaagg ctccgttctc
     3241 aacgggattt cctacgctct tacggaagag tatcaattcg acgacaaagg gcgtatgcgc
     3301 aatacgggat tcaacgatta caagatcttc ggaacgcagg atacgccgga aatccagacg
     3361 attctcgtcc ccacctggga agaaaccggg ccctatggtg ccaagagcgt atccgaaatc
     3421 tgtatcaatg gcgctcttcc cgcgatttcg aatgccatct atgatgccgt aggcgtacga
     3481 ttgcgggaag gcccgtttac gccggaccgg gtcctcgccg ccatccgtca aaaagcctga
     3541 cattcttatc ctgtcaattc ccgcccaccg acaagcttgg tgacggcgaa cgctggttct
     3601 gcccgcaatc aatcactcat ccatccccgt gaagggcaag gggcagtccg caaggtctac
     3661 gcccatgact atgatccaac tgatcccagt gggatacgga agaacgcgcg aagagtcgtc
     3721 gtttgtcgtc gaacggaagt tgttttttaa ttttttcaga ttttcaacca caccccgctt
     3781 caaaccacga gccgatcgaa tggcactgct tggcctggcg attgctttgt acggatgtgg
     3841 ttcctgtgcg gcaacgccgc ccacccagct ttttcccatg tccaagcgcc cttatcgaat
     3901 accgggatac tccgtttgcc tgttcgcgac gcttgtcgga tgcggttctg atccgtccga
     3961 acagagttct tcccctcctc ctgtcgtaga caagcctccg gaagcggtgc ctttgctctt
     4021 ggtggaccgg gccccttccc tattgccatc ggctggaccc gtggaaggag cctgggcggc
     4081 ttttgcagac ctcgatggcg atggtcgtcc cgatatcgcg caacccacct cccaggggct
     4141 actcctatat tggaatggtg aatcgaagtt cgagaaggct tccgcttcgg ccttgccatc
     4201 ggagttcacg gcccccaaaa agccggcgga agacgaggaa cctattgtat ttcaacaagt
     4261 attggcaggg gatttcgacg cggatgggtt gtctgatttg cttatcgtgt ccacgggaaa
     4321 aacgccgttg cgccttctta cccacaagga cgcccggtct tttcaatccc aaacgattgc
     4381 cgttcctgaa ggttccatcc ttgggcatgc ggcggtggcc gatatcgatg gggatgggga
     4441 tacggatgtg attgtgacgt tagggggcga ggcatccggg tcgaccgggg cgcccaacgc
     4501 tcttctgttg atcaacgatg ggaaagggaa gttcgcggat caaagcctgg cgaggctcgt
     4561 ggcaccgggg ctttcgagcc gtggggttgc cgtgggcgat gtggacggag atggcgcgcc
     4621 ggacctcgtt ttttcgggcg ataccacggg acatcgcctg ctgctcaacg atggaaaagg
     4681 atattttcgc gatgcgcccc ccgacgctct tcccgcggga caggctccag ggggaaaaat
     4741 tccagcgatg ggagacctga atggggatgg agccttggac atcctgatcc cttcgtcgca
     4801 agccaatcag gtcttgatca acgatggcaa ggggaatttc accgatgaaa ccgcctttgt
     4861 gcttgggtcg cagccaggaa aagcaaacgt ggcggtcctt gtcgacctcg accgtgacac
     4921 gttgttggac gtggtcttgg cggggccctc cgttcctctg cgaatcctgc gcaacgacgg
     4981 cacggggcga ttgttcgatt attcggccaa catggtgccc caggggccgg ccgcttccga
     5041 ggtggtttcc cttggtgtgg cggacgtgga tggggacaag gatccggacc tattcgtgag
     5101 tcgccaggga ctcgcatcgc cctggctgct ggtcaattgg catccggaac cgatggatga
     5161 cacggatggc gatggtatcc ccgacgaaat cgacaattgt ccgaaagagc ccaatcccga
     5221 ccaagccaat tccgacgcgt atgcgttcaa ttgctccacg gccaggcaat gcaaaacggc
     5281 caccggatgt gtgctgaagg tctttggaga atcctcctat ttgttgtgcg acgagccgaa
     5341 aacctggcag gcggcgcgcg ccttttgccg atcccggggt gcggatctgg tcgtgatcga
     5401 ctccaaggaa gaaaatgaac tgcttgctgc ctcgggcatt cctactgcct ggattggatt
     5461 gtccgaccag caaacggaag gctcgttcct gtgggtcaat gggaaatcgt tgaccacggc
     5521 ctattggaac gacgacgaac ccaatgattc cgggggcgcg gaagattgcg tggggatgtt
     5581 taccagcggc gaaaccgcgg gtttgtggaa tgacttttcc tgcgatgccg agcatgcatt
     5641 tatttgtgag gaaaaaacct tccgcgggcc acaagaccca ggcgatgcct gcgatgtctg
     5701 cccgaacgtc tacgacccca atcaaaaaga ctcggacgcg gatggcgtgg gcgatgcctg
     5761 cacgacggag tcaccggaat gaggagaacg atgcaacgat ggatgcttgt ggccggactt
     5821 gtcgtattgc cgaccggtgg actgctggct gcgtgtgggg atacggagca agtagctgaa
     5881 atcacagtga tttctgtaga gccggcggaa ggctcggctc gtgggggcga gcgtttgatc
     5941 cttcgcgggt cgggattcga tgcgagcgcg cgtgtacgct tcggagaggc ggcggcgacc
     6001 gaggtacgat tcatttccag cagccaactc gaagtcgtca cgcctcttca cctggccggg
     6061 gccgtggata tcacggtgca ggccgcggga gccgaggcaa ctttgcccag gggctttgcg
     6121 tttctgcccc tcgaattatc ctttcgggaa gctcctgcct ggtacttacc cacgttcgaa
     6181 gataccccta ccgatgttct tgcgcaggat ttcgatggcg acggtcatcc ggatttgctc
     6241 ctttcccgcc cgaaccgtcc cgcccttttt cttcccaata gcggcacggg gagtttcgct
     6301 ggtgctacgg tcgtcccgca aacgcctgat gcgggcgtgg atgccgacgc aggacaggcc
     6361 gaaggcggag acggcggcga tttcgagggg gggacggacg cacaacccgc ggaagcggga
     6421 gcgtcggatg ctggagactc gaccggtgcc gctttggcaa agacatggac gcgccatacg
     6481 gcgcggatgt tgtccaccga cattgatggc gatggggacc tcgacgtcat cctttgcaat
     6541 cgggggggcc agcctcatgc tttggccaaa aatataggga aaggcgagtt ttcgatcgtc
     6601 gagggcgcat ttccggaaac atcggatgag tgtcgcgacg ccgtgcttac cgacgtggat
     6661 ggggatggat tggaggatgt cgttgtggtg ggggcgggga aagttggcgg gggaaagtcg
     6721 tatttgaggg tgtatcgtcg ggtttccagc ggcgattccg tttcttttgt agcttccaat
     6781 aatacagaaa aagaggacaa gctggcagaa acaagctgcg ctacggtcac cgggacggcc
     6841 gaggtggagg tatcgtcgaa actatcgcgt acgacagccg cccagggcaa cgtatcgtgt
     6901 cgaattggct ttgaaaccgc ggcggatacg ggaagcctcg aggcctggtt tgcgttgccg
     6961 aaattgccgg tattgctcga tgcgatcacg ttcagcgcca ggctcgaaac cggttcccaa
     7021 gcgcttcgcg tattggtgcg cgatgcgaac ggagaggtat ttgcgtggga tgcggggacg
     7081 gtggatgcca cgggctggaa gtcgatacgt gcttcgaagc tcggttcttg gcaagcgtgg
     7141 gaggggggag atggcgtgat ggatttgcct ctcgatgcgg tgggggtatc gctgtccttg
     7201 tcgtcggcgg cgacatcggc gttgtttttg gacgcgttgg ctctcgaggt gcctgccatc
     7261 ggcaaggtat atatcgatga ttttgagcga aaggagttca cgcatgcgtg gacggacatg
     7321 agaacgcaga tttcggtagg ggatttggat ggggatgggc ttggggattt gttgatcgcc
     7381 agcgcggcgc aaggagcgca atcgccgctc gtattgttgc gcaatgcgtc ggtagcgggg
     7441 gagattgcgt ttcgaccggt tctttccggg gcgctcgagg cgcttaccga accggtggca
     7501 gcgacagcgc tactggatgt ggatgaggat ggggatctgg atatcgtggt cggggtggtg
     7561 gcgggtcagg atcggtactt ttccaatgat gggaaaggct atctattcga cgataccttg
     7621 gcgatgatgc cggtggatcg ggtggatgcg gtgagcatga ccgtggtcga tttggatctc
     7681 gatgggcgtc aggatctgtt gattgccaat gatcgagcgg tcgaccgtgt gtacctgggg
     7741 cggggcgcat cgggatttcg ggatgcgacg ccctg
//
LOCUS       DAOSOR010000246         7772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9363_length_7772_cov_3.970066, whole genome shotgun sequence.
ACCESSION   DAOSOR010000246 DAOSOR010000000
VERSION     DAOSOR010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7772
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9363_length_7772_cov_3.970066"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..593)
                     /locus_tag="PK710_13665"
     CDS             complement(3..593)
                     /locus_tag="PK710_13665"
                     /inference="COORDINATES: protein motif:HMM:NF018627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10819.1"
                     /translation="MSLSMIHPQPVIGLDVDGVLADLVQAVLDAVHQRTGKRFFPEDV
                     QRFDLKSTLGELWTVAHDVLTQPDFVRSLPPYPYAIEGVHRLRQLGRVVFVTTPFAPN
                     PTWSHERSQWLEHHTGAPHHDIAHITDKTLFCGHLLIDDSPAQLQAWTATQRPAIRVV
                     RPWKHDAPGLPARDWHEIVALTAQLLDSPSSRQDPH"
     gene            822..>1184
                     /locus_tag="PK710_13670"
     CDS             822..>1184
                     /locus_tag="PK710_13670"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOT10820.1"
                     /translation="MRHGFAPGRICSHWEDQMRILINGRTVDEDFIERVEEISEQNIK
                     RCMQCGTCSASCPMEADMDLSPRVVMHFTQMGLRDVVQGANTVWICASCHTCEARCPR
                     GVDLPKIMEAIRLLTLRKN"
     assembly_gap    1185..1194
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1286..2188
                     /locus_tag="PK710_13675"
     CDS             1286..2188
                     /locus_tag="PK710_13675"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HOT10821.1"
                     /translation="MRHSMKVSYYPGCTLKTKARNLEDTAIGALAVLGVELVELPRWN
                     CCGAVTSLAQDDLLHMLAPVRDLIRAKEQGSDKLVTICSMCYNTLARANLLMRNDAEK
                     RDALNRFMDEEPDYAGEVEVIHLLNFIRDEVGWDKLRAAVRRPLTGLRVAPYYGCTLV
                     RPAEVAIEPAVDATVFEKFIEALGAQLVHNPGAFDCCGSYQILSHPKQALAASAELLG
                     KAKNKGADVLALSCPLCDYNLGKRQNELVADHGLESKLPTIYFTQLLAVALGLDSSLC
                     HFELNHEGTRSLLEERNLISTVAV"
     gene            2276..3052
                     /locus_tag="PK710_13680"
     CDS             2276..3052
                     /locus_tag="PK710_13680"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOT10822.1"
                     /translation="MSTTAPIGEDKIVAKVTSKHVPIYVMGKRYDVPDSLTIMKAMEY
                     AGYKFIRGCGCRGGICGACGTVYKKPDDYKIHVGLACQTVVQEGMHLTQIPFYPATRT
                     VFDYEQTPAVAEAVFKAYPELFRCVACNACTKICPMDVPVMDYVAAIKRGDFEKATEL
                     SFDCIQCGLCASRCMAEMAQYHIAQMVRRIYGGKIKPKATHLAVMVDNVKAGKFKDGL
                     EQLKKADVATLKQLYMSKDAREIEPNMSEDEWVPTNNAYL"
     gene            3066..4739
                     /locus_tag="PK710_13685"
     CDS             3066..4739
                     /locus_tag="PK710_13685"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOT10823.1"
                     /translation="MPYPQALKELIKKVEATRPERVARKKKGEEVPFMSLDERAATLQ
                     FHPDVKEEGRRALLVGPSKGYRLAHEYADLLEAMPRVDPDKVDLSTPHIETDVLVIGG
                     GGAGTAAALLAQEQGAKVLVATKLRHGDANTMMAEGGIQGCSKGHKDSPYYHYLDVMG
                     GGHFVNVPELVETLVTEAPDVLQWLEHLGMNFSKFPDGRLKTMHGGGTSRKRMHYAAD
                     ITGAEIMRTIRDEARNRAADITVLEFSPAVELILDDQGKCAGALLYNMETEEYLVVRA
                     KAVVMATGGSGRLHVQSFMTTNHYGATFDGVVMAYRAGVPVCFLHTIQYHPTGVVFPE
                     QAEGILITEKFRGIGANLVNVEGNQFVNEREPRDVEAAAIIKECTKKGLGVPTPTGRF
                     GVWLDSPMIEDLHGPGTVEKEFPGKHILYERFGIDISKEPMLIYPTLHYQNGGLEYQS
                     DGSTRIAGLYVAGEVGGGVHGENRLMGNSLLDILVFGRLAGAAAGVYAKAKGASLGKL
                     SLDHVRVFTKELQESKVGADRVSPLLLPDYTLLDTKSRQLTSHYVGSLR"
     gene            complement(4742..5893)
                     /gene="argE"
                     /locus_tag="PK710_13690"
     CDS             complement(4742..5893)
                     /gene="argE"
                     /locus_tag="PK710_13690"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003474.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HOT10824.1"
                     /translation="MVAPDRTEMMRALVATASASSFDPAKDEGNQGVIDLLASWLSDL
                     GFSIELQTLSGTAASRSNLIARLGDGPGGLVLAGHADTVACEHGRWSFDPYRLTLDHG
                     RYYGLGIADMKVFFAVAIEAAQRFSRHSLREPLYLVATADEECAMLGAHKLVEAGKPR
                     ARWAVLGEPTSLEPVRMHKGMMMTRVRLTGKSGHSSDPKGGASALDGMLAVCNAMVAW
                     REELASHRDDNFRVAIPTLNLGTLHGGDHPNRICGQCELQMDLRPLPGMDITRLRTTM
                     RERIEKAIAFHDLQLEIDALYPGTEPLCTRSESPLVQFAELVTEKRATSAGYGTEAPH
                     YARLGMDVVVLGPGDIAQAHQPDEYVQASRLDPMVDILSRIIRRFCVED"
     gene            6085..7062
                     /locus_tag="PK710_13695"
     CDS             6085..7062
                     /locus_tag="PK710_13695"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT10825.1"
                     /translation="MMLWLTWAGLSCSLDRSGKSAGGISPHDAAAPETEIPDSDFPIV
                     DVMDDAPQKVDGQAGSSGQGDSGVGGNGQAGSSVQAGAAGSAIDAGINVVCPDKGGKM
                     VALPGGVCIDKTEVTRAAYSAWQPMAVKDMQPKECQWNESVEPDPQCMNHPKDVCKKD
                     CDEHPQVCIDWCDAWLFCRSHGKRLCGSWSTASSTDIFDDYDDEEKDVWYYACSSGGA
                     NDWTFGDQVPEPRPCNFGHNSDSGSTVAAGSMMDCQSPNPLFAGVFDLTGNAAEWQDS
                     CAASKGKKDDCRLRGGSYDRGAPSNRCDSERVEKRDTASFATGFRCCWP"
     gene            7121..7453
                     /locus_tag="PK710_13700"
     CDS             7121..7453
                     /locus_tag="PK710_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HOT10826.1"
                     /translation="MTIQIFGSPKCKRTRAAERFFKERKIRVQMIDLAEQNLSPGEFD
                     AVKNHVGLEAMIDASSKEYKRLGLQHLSFDLEQKLREHPLLLMTPVVRHGSEVTVGAK
                     PDVWKTWI"
     gene            complement(7585..>7772)
                     /locus_tag="PK710_13705"
     CDS             complement(7585..>7772)
                     /locus_tag="PK710_13705"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10827.1"
                     /translation="ELRDDRALFFVDHMAAGMYHYRYLARATTIGRFVMPSTRVESMY
                     QPEIFGRTGATMIDVKP"
BASE COUNT         1746 a         2000 c         2355 g         1661 t
ORIGIN      
        1 ggttagtgtg gatcttggcg ggacgatggt gaatcgagca gttgagcggt gagggcgacg
       61 atttcgtgcc agtctcgagc gggcagacct ggagcgtcat gcttccaagg tcgaacgacg
      121 cggatggcgg ggcgctgtgt ggctgtccat gcttgcaact gcgctgggga gtcatcgatg
      181 agtaggtgac cgcaaaacag ggtcttgtcg gtgatgtggg cgatatcgtg gtggggagcg
      241 ccggtatgat gctcgagcca ttgcgagcgt tcgtgagacc aggttgggtt gggagcaaac
      301 ggggtggtga cgaacacgac gcgtccgagt tggcgcaagc gatggactcc ttcgatggcg
      361 taggggtaag ggggcagcga gcggacgaag tctggttgtg tgaggacatc atgggcgacg
      421 gtccatagtt cacccagggt acttttcagg tcgaagcgtt ggacgtcttc gggaaagaac
      481 cgtttgccgg ttctttggtg aacggcgtcg agaacggctt gaacgaggtc agcgaggacg
      541 ccgtcgacat cgaggccgat aacgggttgt ggatgaatca tgctgaggga cacggtgagc
      601 gaggaagcag catctagtcg atgggaatat tgttagtcga ggctaatcat cgataggcag
      661 accaaaggaa aaggaaggca aaagcaatga tcagtggctt gccgacgtct tgtgattgaa
      721 gtaacgaaaa agagtccttg gcgcatgtgg atcgtcagct tgaatctcaa tgaggattgg
      781 gcgacaggat gacggattcg tggcgcgaag aggagagact catgaggcat gggttcgctc
      841 cgggacggat ttgtagtcat tgggaggatc agatgcgtat tctcatcaac ggtcgcacgg
      901 tggacgagga cttcatcgag cgcgtcgagg agatcagcga gcagaacatc aagcgttgca
      961 tgcaatgcgg cacgtgttcg gcgtcatgtc cgatggaagc ggacatggat ttatcgccgc
     1021 gcgtggtgat gcattttacg cagatgggtt tgcgcgacgt ggtgcagggt gcgaatacgg
     1081 tttggatttg cgcttcttgc catacgtgcg aggcgcgctg tcctcgaggg gtcgatctgc
     1141 cgaagatcat ggaggccatt cggctactga cgcttcgtaa gaacnnnnnn nnnnaggatg
     1201 cggtcgatcc gaggcagctc gaggcgacgg tcattgatga agctccgcaa gcggcgatgg
     1261 ttggctgttt tcgcaaactc acggcatgag gcattcgatg aaagtaagct actatcctgg
     1321 ctgtacgctc aagaccaagg cgcgcaatct cgaggacacg gcgatcggag cgttggcggt
     1381 attgggggtc gagcttgtcg aattgccgcg atggaactgt tgtggggcgg tgacatcgct
     1441 agcacaagac gatcttctgc atatgttggc gccggttcgt gacttgattc gtgcaaaaga
     1501 acaaggaagc gacaagctcg tgaccatctg ttcgatgtgt tacaacacgt tggctcgggc
     1561 gaatttgctg atgcgcaacg atgcggagaa gcgcgatgcg ctcaatcggt tcatggacga
     1621 ggagccggat tatgcaggcg aggtggaggt tatccatttg ctcaacttca ttcgggatga
     1681 agtcggatgg gataaacttc gcgcggcggt caggcgtccg ttgactggct tgcgggtggc
     1741 gccttactac ggatgcacgt tggtacggcc cgcggaagtg gcgatcgagc cagcggtgga
     1801 cgctacggtg ttcgaaaaat tcatcgaagc cttgggcgca cagctcgtcc acaatcccgg
     1861 cgcgttcgac tgttgcggtt cctaccaaat cctgagccat cctaaacaag cgctcgcggc
     1921 ttccgccgag ttgctcggaa aagccaaaaa caagggagcc gatgtgctcg ccttgagttg
     1981 tccgttgtgt gattacaatc tcggaaaacg gcaaaacgaa ctcgtggccg atcacggtct
     2041 cgaatccaaa ctaccgacga tctacttcac acaattgctt gccgtggcat tggggctcga
     2101 ttcgtccctt tgccacttcg aactcaatca tgagggaacg cgttccctgc tcgaagagcg
     2161 aaacctgatt tcgacggttg ctgtttgatg gttttgctgg cgcttcgatg gagtgtcagc
     2221 gagcttgggt accaccatcc ccggggtggg gtgagagctt gaacgagagg tctagatgag
     2281 caccactgca ccgattggcg aggacaaaat tgttgccaag gttacctcga agcacgtgcc
     2341 aatctacgtg atgggcaagc gctatgacgt gcccgactcc ctcaccatca tgaaggcgat
     2401 ggagtatgcg ggatacaagt tcatccgagg ctgtggatgc cgtggcggca tctgtggcgc
     2461 ctgcggaacc gtgtacaaaa aaccagatga ctacaagatc catgtcgggc ttgcgtgtca
     2521 gacggtggtg caagagggaa tgcatctcac gcaaatcccg ttctatcccg cgactcgaac
     2581 cgtcttcgat tacgaacaga ctcctgccgt agccgaggcc gttttcaagg cctatccgga
     2641 actgtttcgc tgtgtggcct gcaatgcttg cacgaaaatc tgcccgatgg acgtgcccgt
     2701 catggactac gtggccgcca tcaaaagagg cgacttcgaa aaggccacag agctttcttt
     2761 cgactgcatt caatgtggcc tttgtgcgtc acgctgcatg gccgaaatgg cgcagtatca
     2821 catcgcccag atggttcgtc ggatttacgg gggcaagatc aagcccaagg caacccatct
     2881 tgccgtgatg gtcgataacg tcaaagccgg gaagttcaaa gacggtctcg aacaactcaa
     2941 aaaagccgat gtagccacac taaaacaact ttatatgtct aaggacgctc gcgaaatcga
     3001 accgaatatg tccgaggacg agtgggttcc caccaacaac gcatacctct gaggagtgat
     3061 tggtcatgcc ttacccgcaa gctttgaaag aactcatcaa gaaggtggag gcaactcgtc
     3121 ccgagcgcgt ggcgcgaaaa aagaagggtg aagaagttcc cttcatgtcg ctcgacgaac
     3181 gagcggccac gttgcaattt cacccggatg tgaaagagga agggcgtcgc gcattgctcg
     3241 tcggaccgag caaaggatat cgcctcgccc atgaatacgc ggatctgctc gaagcaatgc
     3301 cccgcgtaga cccggacaaa gtcgaccttt ccaccccaca tatcgaaaca gatgtcctcg
     3361 tgattggagg tggaggcgct ggcaccgcgg ctgctcttct cgctcaagag cagggagcca
     3421 aagtccttgt cgccaccaaa ctccgtcacg gtgatgccaa caccatgatg gccgaagggg
     3481 gcattcaagg atgctccaag ggacacaaag actcgcctta ctatcattac ctcgacgtca
     3541 tggggggcgg ccacttcgtc aacgtcccgg aactcgtgga aaccctcgtt acggaagctc
     3601 ctgatgttct ccaatggctc gagcacctcg gcatgaactt ctccaagttc cccgatggac
     3661 gtctcaagac catgcacggt ggtggcacct cgcgaaaacg gatgcattac gccgccgata
     3721 tcaccggcgc cgaaatcatg cgcacgattc gcgacgaggc tcgtaatcgc gccgcagata
     3781 tcacggtcct cgagttctcg cccgcggtcg aactcatcct cgacgatcag ggcaagtgtg
     3841 ctggtgcttt gctatacaat atggagaccg aagagtactt ggtcgtgcgc gccaaagcgg
     3901 ttgtgatggc gacgggcggc agcgggcgtt tgcatgtcca gtccttcatg acgaccaacc
     3961 attacggcgc caccttcgat ggtgtggtga tggcgtatcg tgccggggtg cccgtttgct
     4021 tcttgcacac gattcaatat cacccgaccg gggtcgtatt ccctgagcag gccgaaggta
     4081 tcctcatcac tgaaaaattc cgcggtattg gagccaacct cgtcaatgtg gaaggcaatc
     4141 aattcgtcaa tgagcgcgaa ccgcgcgatg tcgaagccgc ggccatcatc aaggagtgta
     4201 cgaaaaaggg acttggcgtg cctacgccca ctggccgctt cggcgtgtgg ctcgactctc
     4261 cgatgatcga agaccttcat ggtccgggaa ccgtggaaaa ggagttccct ggcaagcata
     4321 tcctttacga acgtttcggt atcgatatct ccaaagagcc catgctcatt taccccacgc
     4381 tccattatca aaacggtgga ctcgaatacc aatccgacgg ctcgacccgt atcgctgggt
     4441 tgtacgtggc gggagaagtc ggcggtggcg tgcatgggga aaaccggctc atgggcaatt
     4501 ccttgctcga cattctcgtg ttcggccgac tcgctggtgc ggccgcgggg gtgtacgcaa
     4561 aagcaaaagg ggcttcccta ggaaaactat ccctggacca cgttcgtgtc ttcaccaaag
     4621 aacttcaaga aagcaaggta ggagccgacc gagtctctcc cttgttgctc ccggactata
     4681 cgcttctgga taccaaatcc cgccagctca ccagccacta cgttggcagt ctgagatagc
     4741 ttcaatcctc aacacagaat ctccgtatta tccgactaag gatatcgacc ataggatcga
     4801 ggcgcgaggc ttgcacatac tcatccggtt gatgggcctg ggcgatgtcg ccagggccca
     4861 acacgacgac gtccatgcca agccgtgcgt aatggggagc ttctgttccg tagcctgctg
     4921 aagtcgcacg cttttccgtg actagttccg cgaactgcac gaggggagac tccgaccggg
     4981 tacacaaggg ttccgttccc ggatacagag catcgatctc gagttgcagg tcatgaaaag
     5041 caatggcttt ttcgattcgt tctcgcatcg tggttcgaag acgcgtgata tccattccgg
     5101 gaagcggtcg aagatccatc tgcaactcgc attgtccgca aatccgattc ggatgatcgc
     5161 caccatgcaa cgtcccgaga ttgagcgtgg gaatggccac acgaaagttg tcatcgcggt
     5221 gggaagcaag ctcttcgcgc cacgcgacca tggcattgca tacggcgagc atgccatcca
     5281 gcgcgcttgc tccccccttg ggatcgcttg aatggcctga tttacccgtc aggcgcaccc
     5341 gtgtcatcat catgcctttg tgcatgcgga ccggttcgag cgatgttggc tcccccaaaa
     5401 cagcccaacg cgcgcgtggt ttccctgctt ccacgagctt gtgggcgccg agcatcgcgc
     5461 attcttcatc cgcagtagcc acaaggtaca gaggttcgcg aaggctatgg cgtgagaatc
     5521 gttgagcggc ttctatggca acggcgaaga aaaccttcat gtcggcaatg ccaagcccgt
     5581 agtagcgacc gtgatccaag gtaaggcggt agggatcgaa ggaccatctt ccgtgttcgc
     5641 aggctaccgt atcggcatgg cccgcaagca cgaggccgcc agggccatcg ccaagccgcg
     5701 cgatgagatt gctgcggctc gcagcagtac cagatagtgt ttggagttcg atggagaatc
     5761 caagatcgga taaccaagaa gcgagaaggt caatgacacc ctgattgcct tcgtctttgg
     5821 ccggatcgaa actgctcgcg gaggcggtgg ccacgagcgc tcgcatcatt tcggttctat
     5881 cgggagcgac catggcgcca tgttggcata tttcccaact tcgagctttt gttctccgca
     5941 acaagagcgg ggaaatgact ttcctggttt tggggtagac tgtggtttgt ctatggaatg
     6001 gatggctgtc agggtacgct cgagggtgat gacatgagag gctatgggcg aagttcgatc
     6061 cggttgcttc atccttgcat cttgatgatg ctttggttga cctgggcggg actgtcttgt
     6121 tcgctcgacc gatccgggaa aagcgcgggc ggcatttccc ctcatgatgc cgcggcacca
     6181 gaaactgaga taccagactc ggacttcccg attgtggatg ttatggacga tgcgccacag
     6241 aaggtggatg gccaggccgg ctccagtgga cagggcgaca gtggcgtggg tggtaatgga
     6301 caagccggtt caagcgtcca ggctggtgcg gctggcagtg cgatcgatgc gggtatcaac
     6361 gtcgtatgtc ccgacaaagg ggggaagatg gtggctctgc cgggaggcgt atgcatcgat
     6421 aaaacggaag tgacccgcgc ggcctatagc gcctggcagc ccatggcggt caaggatatg
     6481 caaccgaagg agtgccaatg gaatgagtcc gttgaaccag atccacagtg catgaatcac
     6541 cccaaagacg tgtgcaaaaa agactgcgac gaacaccctc aggtctgtat cgattggtgt
     6601 gacgcatggt tgttttgccg ttcccatggc aaacggttgt gtgggtcatg gtctaccgct
     6661 tcgtcgacgg acattttcga tgactatgac gatgaagaga aggatgtatg gtattacgcc
     6721 tgttcatcgg gaggcgcgaa cgactggacc ttcggcgatc aggtccccga accgagaccc
     6781 tgcaatttcg gccacaattc ggactccggt tccaccgtag ccgccggatc gatgatggat
     6841 tgtcaatcgc caaacccact atttgccggc gttttcgatc tgacgggcaa tgcggcggaa
     6901 tggcaagact cctgtgccgc aagcaaggga aaaaaagatg attgccgttt gcgcggcggc
     6961 tcttatgacc gtggagcccc gagcaatcgc tgcgattcgg agcgggtcga aaaacgggat
     7021 accgcttctt tcgctaccgg attccgatgt tgctggccct gaccaccttg ctcttgcgat
     7081 acgatggctg gatgtatcaa aaacgatgag ggtggacgtc atgacgatcc agattttcgg
     7141 ctctcccaaa tgtaaacgga cgcgagcggc cgaacgcttc ttcaaggaac gaaaaatccg
     7201 cgttcagatg atcgatctgg ccgaacaaaa cttgagtcct ggagagttcg atgccgtcaa
     7261 gaatcacgtt ggactcgaag cgatgatcga cgcatcgagc aaagaataca aaaggctggg
     7321 tctgcaacat ttgagtttcg acttggagca gaaactgcgg gagcatccgc tgctgcttat
     7381 gactcccgtg gtgcgtcatg gatcggaagt gacggtggga gccaaacccg atgtgtggaa
     7441 gacgtggatc tgatcaccga atccgctatc gaccccacgg atggagcaat ccttccgtgg
     7501 gtttcgagat gtctcctttg cgatgtaact atttgatatt gttgataatt taaggaaagc
     7561 ggacggctgg ggaacggtga ggcgtcatgg cttcacgtcg atcatcgtgg ctccggttcg
     7621 accaaatatt tcgggctggt acatcgattc aacacgcgtc gaaggcatga cgaatcgccc
     7681 gatggtcgtg gctcgagcga ggtatcgata gtggtacatg cccgcggcca tgtggtcgac
     7741 gaaaaacagc gcgcggtcat cgcgcagttc ct
//
LOCUS       DAOSOR010000247         7764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9376_length_7764_cov_4.218835, whole genome shotgun sequence.
ACCESSION   DAOSOR010000247 DAOSOR010000000
VERSION     DAOSOR010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7764
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9376_length_7764_cov_4.218835"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="PK710_13710"
     CDS             <1..808
                     /locus_tag="PK710_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10828.1"
                     /translation="VRLDEKLKISQPVRDVHVFWDHILVRGANRFDETWLAAACIKPG
                     ATGIEAPVDVGMLPESGANRPPTETDLPMVGCRTDKTKVVRVRGDSHDFLSFFVADKW
                     TKPVKTSRTGGDLSCRRVEAYLTRFDSPAGAGMLESSIVVEKCSPAACQTSELTIEEM
                     FKGTLGLAPASAPLIAQIEGKILVVWTAAERGGLRLRLAPIDALAKTPDTVLFDDLVQ
                     EGKVQKTSSIAEVRLLARASFAVLLIRTSQGLHALHIDAEGRMQLLKVES"
     gene            complement(883..2085)
                     /locus_tag="PK710_13715"
     CDS             complement(883..2085)
                     /locus_tag="PK710_13715"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOT10829.1"
                     /translation="MEAQGEGMKYGHGEKTVLLLAASVIVIAGVKIVGPLLVPLALAV
                     VITAVSLPVVRFLERMRFPSPVAILLAVLLDVSVVGALLSLLGNSLGAFYNALPRYQD
                     RLTHVLRNDVVWLDQHGIHLSREFNEHVSTFGKVIPMVGSLVSSVASAVSTTLFVFLL
                     VVFLLFEINRWQVKIRYAIGDPGADLRRFTTAARELQKYLFVKTAISAATGLLAGIWV
                     AVLGLDFPVLWGLLAFVLSYIPTIGSILAGIPPILLAWIQLSPGAAIAVTAAYLLLTF
                     LFGSVIEPRVMGRALGLSPLVVLISVVFWYWIWGPVGALLSAPLTMGVKIALSNTPDV
                     RWAAIMLGSTRWVEEQQSEWEHDKPVLTPRPGQFCLPSSAQSRRTDSIVAPPPASRPS
                     SKISCNYK"
     gene            2268..3476
                     /locus_tag="PK710_13720"
     CDS             2268..3476
                     /locus_tag="PK710_13720"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOT10830.1"
                     /translation="MTGTFEAFQISDRVFWVGAIDWSIRDFHGYLTDRGTTYNAFLIK
                     GDQKNVLVDTVKKPFYEEMYSRIASVIDPSTIDTVISHHAELDHSGSLPALVAAVRPT
                     NVLTSKAGVDALSQHFSLDVPVQAVPSGTTMELGDIRLTFHDTRMLHWPESMVSWLDG
                     DDILFSQDGFGMHLASSQRWTDELNAELIHRETAKYYANILMPFAGPVSKVVGKLGPV
                     IQKAKIIANEHGPMWRSGIEELLGWYNRWSERKPTRKVVITYASMWQSTATMARAIAE
                     GAAQGGMQVALMGLDTAHRSDIAMELLDAGALVVGSPNLNGFMYPTVADLLTYLKGLK
                     PKGLVGAAFGSYGWSPAALDQVQAALQEIKMDLVAEPLGVKYVPTTQDWQACRQLGKT
                     VAERVQSLCG"
     gene            complement(3492..5198)
                     /locus_tag="PK710_13725"
     CDS             complement(3492..5198)
                     /locus_tag="PK710_13725"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOT10831.1"
                     /translation="MRRAIYITVLFGVTVTACGSNSDPSPHKASQDAGIDTTDLDVQS
                     DDAFEASDDSSPDVPADVVEDVVQQDVIQDAADPRFEPIVDSLEKERMKFGAPGVALA
                     IVEGGKLSFSTGLGSKGENNNDPVHDTTLFRIASITKPLTATAVLQLVERGDIDLEQP
                     ITTYLPTLDFAKDPSWASSIRIKHLLTHESAIVDALEIDSTRSDDHLKEVMLGPYAEN
                     GYLMAPPGRMYNYSNPGYMFAGLVAETVGGDYYRLRMHDEVLLPQGMDRTYFLPSEVL
                     ADGDYALGKTVHWTTGQPLTVEPDTYDNAWGRPAGYAWSSVLDLAKFIQFLRDGNDGV
                     LAHSLREEMMKPQVSTKEFLDRLAYGYGLMVQQGAFLGSFYDIKIVQHAGALPGFAAD
                     MYWVPDCDLGFITLANTDGAYFISTFVLALQTLCSLPAPTTPPDITVNPSTFTQFEGV
                     FHDPFNVGTMVVTKDGNDLNIELPTLDDLDIPYEHRLTPTLPNNFLFYVQGIPLPLTF
                     LFDDQGQVEYLRTRPFVGKKAEQDGGTPPPPPPIHKLSRNGLLQRFHYSNIERSSVIF
                     RR"
     gene            complement(5230..7383)
                     /locus_tag="PK710_13730"
     CDS             complement(5230..7383)
                     /locus_tag="PK710_13730"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HOT10832.1"
                     /translation="MTLLPLEQVLRAARCPRLFILEKEGVPVQDEGQVVLHDALSQTL
                     DHLVTTAHRNDSLLSSLAAIPPSEKDLARACWRHAYDYIYPRLIPLLPQIDGDTLYQV
                     DQLLRSACATFAELLGAAAGLAQSAQEAIDKTLSLSDPTLRVDLGSVVVEGSVAMACC
                     HAPTAIPWIVNTSFQRPEPRSHEVLGMIYALDRKARNGKSSIVLVQIQPDDVRRLEVS
                     HPDITQQTQLVTWVSQLERWREQDATGPADTTICKTCPAQQPCWTRWGRTLPDVEQLP
                     RRGSVARSHRPPPASRANSESASPRAQTALTPLPIWLGQERETGKAARLEPSDIQKHL
                     AIFGTSGSGKTCLAKCIIEEAAIAGIPCIVFDRQGDLVQLAATATLGDDDLTSRKKAW
                     LEHAQIRLYTPGSDVGQRTSLRPLWFPEPTLDEATRRLCRIAMAEVLLGGLPVPETWA
                     SLAQEYLAQVLETPSGSSSLAGLITQLRNPSELRVEPFLRSRTRRETLALLIQSLLDG
                     KLGPLFTQGPRLRIEQLVESTTPGKAPVNVIWLPGLGDALTQQRFVITLLVDVFTWMA
                     RQESSSRRLLIHIDEASPFLPPMGDPSSKQLLRKLLQEDSRGGVGLLLGSHNLTDVDY
                     KLLAQSGTVLLGRLGSTLDKARVRKMLPPSGGFDPTGAAEMLTNATVGRFLTSRISRS
                     NTPLVLQARMPITYHGRPWGEPEIRKHVQPNEQDS"
     gene            complement(7380..>7764)
                     /locus_tag="PK710_13735"
     CDS             complement(7380..>7764)
                     /locus_tag="PK710_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10833.1"
                     /translation="APLAAFEQLLNAAATGDVPLAEEEIRRAVFDIDAIDRVVGNLIG
                     AAFTDDGVSRGDTRQWQETILALLRNRAAFISLPKLVRELEAPAEAIHQATEALVSRG
                     LAHVALDRKRIPIVMLRPEAMVETA"
BASE COUNT         1719 a         2042 c         2375 g         1628 t
ORIGIN      
        1 ggtcaggctc gatgagaagc tcaagatatc ccaacccgtg cgtgatgttc atgtgttttg
       61 ggatcatatt ttggtgcggg gcgcaaatcg attcgatgag acctggttgg cggcagcgtg
      121 tatcaaaccg ggagccacgg ggatcgaagc cccggtggac gtagggatgc tgccggagtc
      181 gggagccaat cgtcctccca ccgaaacgga tcttccgatg gtgggatgtc gaaccgacaa
      241 aacgaaggtg gtgcgagtgc gcggtgatag ccacgatttc ttatcgtttt tcgtggccga
      301 taagtggacc aagccggtga aaacatcccg aacgggaggc gatctttcgt gtcgtcgtgt
      361 cgaagcgtac ttgactcgtt tcgattctcc tgccggagct ggaatgctcg agtcgtcgat
      421 cgtggtggag aagtgctcgc cggccgcgtg tcaaacttct gaacttacga tcgaggagat
      481 gttcaagggc acgctgggtc ttgcgcccgc gtccgcgcca ttgatcgcgc aaatcgaagg
      541 caagatactc gtggtgtgga ccgccgccga gcgtggtggt ttgcgattgc gcctggcacc
      601 aatcgatgcg ctcgcgaaaa ccccggacac ggtgctattc gacgatctgg tgcaagaagg
      661 aaaggttcaa aaaaccagca gtattgcgga ggtgcgactc ctggcacggg caagcttcgc
      721 cgtgctgctc atcagaacgt cccaagggct tcatgcgttg catatcgacg ccgaagggcg
      781 catgcagctt ctcaaggttg aatcctgaga gggagtgaaa accctgttgg gtacgaaagg
      841 cacctgccgg tgtgacgaaa cagattgtcg gtatatagct aactacttgt aattacacga
      901 tattttagag cttgggcgcg acgctggagg gggagcgacg atgctatcgg ttcgtctgct
      961 ttgcgccgac gagggaagac agaattgtcc cggtcgcggc gtgagaacgg gtttgtcgtg
     1021 ttcccattcg ctttgttgtt cttcgaccca gcgagtggaa cccagcataa tggccgccca
     1081 tcgaacatcg ggggtgttgg aaagcgcgat tttcacaccc atggtgagcg gtgcggaaag
     1141 caaggcgccg acgggacccc aaatccagta ccaaaaaacg acggagatga gcacgacgag
     1201 gggggataat cctagggcgc ggcccatgac tctgggttcg ataacgcttc cgaacaggaa
     1261 ggtgaggaga aggtaggcgg cggtgacagc gatggccgct ccggggctga gttgaatcca
     1321 tgcaagcaat atggggggaa tcccggcgag aatgcttccg atggtgggga tatagctgag
     1381 cacaaaggca agaagtcccc agagcacggg aaagtcgagc ccaaggacgg ccacccagat
     1441 tcccgcgagc aatcccgtgg cggcgctgat ggcggttttg acgaacaagt atttttgcag
     1501 ctctcgtgcc gctgtggtga accgacgaag gtctgctccg ggatcgccga tggcgtagcg
     1561 aattttgact tgccaacgat tgatctcgaa cagcaggaac accacgagca aaaacacgaa
     1621 aagcgtggtg gacacggcgg atgccacgct cgacaccagc gagcccacca tcggaatcac
     1681 cttgccgaag gtagagacat gctcattgaa ctcgcgggaa agatggatcc cgtgttgatc
     1741 caaccacacc acatcattgc gcaacacgtg ggtgagacga tcttgataac gaggcaaagc
     1801 gttatagaaa gctccgaggg agttgcccaa caaggacaaa agggcgccga ccacgctcac
     1861 atcgagcaac accgcgagaa gaattgccac gggggacggg aagcgcattc gttcgaggaa
     1921 gcggaccacg ggtagggaca cggccgtgat gacgaccgcc agggcaagag ggacgagcaa
     1981 gggtcccacg attttgactc cagcgatgac gatgaccgaa gccgcgagca gaagaaccgt
     2041 cttttctccg tggccgtatt tcatcccttc gccttgcgct tccatgcgcc gaaatccatg
     2101 agcagcgtag cccaattgga tcgcggacgc acgtcatctg cgtgtcgtcg cgtcgttgca
     2161 acggctcgaa gcatgagtca ggaaggatat gtctggaacc tggaggcgcg acgtggcatc
     2221 gaagggctga acgagggccc gtttcccttt accaaggagt gctatcgatg accggcacgt
     2281 ttgaagcctt ccagatatcc gaccgggttt tttgggtagg agccattgat tggtctatcc
     2341 gcgactttca tggatacctc acggatcgcg ggacgaccta caatgcgttc ctcatcaaag
     2401 gagaccaaaa gaacgtactg gtcgataccg tcaaaaagcc tttctacgaa gagatgtatt
     2461 cacgcatcgc ttccgttatc gatccttcca ccatcgatac ggtgatttct caccacgccg
     2521 aactcgacca ttccggaagt cttcctgcgt tggtagccgc cgttcgtccg acgaatgtgc
     2581 tgacgtccaa agcgggggtc gatgcgcttt cccagcattt ttcgctcgat gtacccgtgc
     2641 aagcggtacc gtcgggcacc acgatggagc taggggacat tcggctgacc ttccatgaca
     2701 ctcgaatgct tcactggccc gaaagcatgg tgtcctggtt ggacggcgat gacattctgt
     2761 tttctcaaga cggattcggc atgcatctgg cgtcgtcgca gcgatggacc gacgaactca
     2821 atgctgaact catccatcgg gaaaccgcga agtactacgc caacatactc atgcctttcg
     2881 cgggacccgt atcgaaagtg gttggcaaac tgggtcctgt cattcagaaa gccaagatca
     2941 tcgccaacga gcacgggccg atgtggcgtt cgggcatcga ggaattgctg ggttggtaca
     3001 accgatggag tgagcgaaag cccacgcgca aggtggtgat cacctatgct tcgatgtggc
     3061 agagcacggc aaccatggcg cgcgcgatcg cggaaggtgc ggcgcaaggt ggcatgcaag
     3121 tggccttgat gggattggac accgcccacc gatccgacat tgccatggaa ctgctcgacg
     3181 ccggtgcgct cgtggtgggt tctcccaacc tcaatggatt catgtatcct accgtcgccg
     3241 accttctcac ctatctcaaa gggttgaaac ccaagggcct ggtcggtgcc gcgtttggat
     3301 cgtatgggtg gagccccgcc gccttggatc aagtgcaagc agcgctgcaa gaaatcaaaa
     3361 tggatctcgt ggctgaaccc ttgggggtca agtacgttcc caccacacaa gactggcaag
     3421 cctgccgaca actcggaaaa accgttgccg aacgcgttca atccctttgt ggatagatgc
     3481 gtagcgctgg attaacgtcg aaatattacg gaacttctct cgatattcga ataatggaac
     3541 cgctgaagca gtccgtttcg ggatagcttg tggatcggag ggggcggagg gggagtccct
     3601 ccgtcttgct ctgccttttt gcccacgaac ggacgagtcc gcaagtattc cacctgaccc
     3661 tggtcgtcga acaagaacgt cagcggcagc ggtattcctt ggacatagaa caagaaattg
     3721 ttgggaagcg tgggagtgag acgatgttca tagggaatgt caaggtcgtc tagcgtgggc
     3781 aattcgatgt tcaaatcgtt gccgtctttg gtgacgacca tcgttcccac gttgaaggga
     3841 tcatggaaga ctccctcgaa ttgggtgaag gtcgaaggat tgacggtgat atcgggtggt
     3901 gtggttggcg ctggaagcga acacagagtc tgcagcgcca agacgaacgt actgatgaag
     3961 tacgctccat cggtgttcgc gagtgtgatg aaaccgaggt cacagtccgg aacccaatac
     4021 atatcagccg cgaaacctgg tagcgcacca gcatgttgta cgatcttgat atcgtaaaac
     4081 gaccccaaga aagcgccttg ctgcaccatc aacccgtagc cataggccag acgatccaaa
     4141 aactccttcg tgctcacttg aggcttcatc atctcctcac gaagagaatg agcgagcacg
     4201 ccgtcgttgc catcccgtaa aaactgaatg aatttagcta gatccaagac agaagaccag
     4261 gcgtaccccg ccggcctgcc ccaggcgttg tcatacgtat cgggctccac cgtcagcggt
     4321 tgccccgtcg tccaatgaac cgtcttgccc aaggcataat caccatcggc caatacctcc
     4381 gatggcaaga aatacgttcg atccatcccc tgaggcaaca acacctcgtc gtgcatccga
     4441 aggcgatagt agtctccgcc caccgtttcc gcgacgagac ccgcgaacat atatccgggg
     4501 ttcgagtagt tgtacatgcg cccgggggga gccatcaaat agccattttc agcgtagggt
     4561 ccaagcatga cctctttcag gtggtcatcg gatcgggtgc tgtcgatttc cagggcgtcc
     4621 acgatggcgg attcatgagt caatagatgt ttgatccgaa tcgacgaagc ccacgaaggg
     4681 tccttcgcaa aatcgagggt cggtaagtag gttgtgatgg gttgttcgag atcgatgtct
     4741 ccacgctcga cgagttgaag cacggcggtg gcagtcaagg gtttggtgat ggaggcgatg
     4801 cggaacagtg tggtgtcatg aaccggatcg ttgttgttct cccccttgct ccccaacccc
     4861 gtggaaaagc taagttttcc cccctccaca atggccaggg ccacgccagg agcgccgaat
     4921 ttcatgcgct ctttttccaa ggaatcgaca atgggctcga agcgaggatc ggctgcgtcc
     4981 tggatgacgt cctgctggac gacgtcctcg acaacgtccg ctggcacatc gggcgaagag
     5041 tcgtcgctgg cctcgaaggc atcatccgat tgaacgtcga ggtccgtggt atcgatgccg
     5101 gcgtcttgcg aagctttgtg gggcgaagga tccgagttgg agccgcaagc agtcacggta
     5161 acgccgaaca acacagtgat atagatggct cttcgcatgg gtcacagcgt gtcatgcatg
     5221 acgtttgcgt caagagtctt gctcatttgg ctgcacgtgc tttcgtatct ccggctcccc
     5281 ccaagggcga ccatgatagg taatgggcat ccgtgcttgc agcaccaacg gggtattgga
     5341 tcgactgatt cgggaggtga gaaaacgccc gaccgtcgcg ttcgtgagca tttcagccgc
     5401 ccccgtgggg tcgaaaccac cgcttggggg aagcatttta cgaacgcgcg ccttgtccag
     5461 ggtcgatccc agacgtccga gaagcactgt cccagattga gcaagaagct tgtaatcgac
     5521 gtccgtgagg ttatgggaac ctagcagcaa ccccacgcca ccgcgtgagt cttcctgaag
     5581 aagttttcga agcagttgct tcgacgatgg gtctcccatg ggaggaagaa aaggactcgc
     5641 ttcatcgata tgaatgagca ggcgccgcga actggactct tgcctggcca tccacgtgaa
     5701 cacatccacc aatagcgtga tgacgaaacg ctgttgcgtg agtgcatccc caagccccgg
     5761 caaccagatc acgttgacag gagctttccc tggcgtggtc gattccacga gttgttcgat
     5821 acgaagacgc ggtccttgtg tgaagagcgg gccgagcttc ccatcgagaa gggattgaat
     5881 cagcaatgcc aacgtttctc gtcgcgttct ggaacgaaga aagggctcga cgcgcaattc
     5941 cgacgggttt cgcaattgcg tgatgagacc cgcgaggctg gaggaacccg atggggtttc
     6001 aaggacttgc gcgagatact cctgggcgag gctggcccag gtttctggaa cgggtaaccc
     6061 tccgagcaac acctccgcca tggcgatacg acaaagtcgt cgtgtggcct catcgagggt
     6121 gggttcggga aaccacaaag gacgaagaga agttcgctgt ccgacgtcgg accctggtgt
     6181 gtacagtcgg atttgggcgt gttcgagcca tgcttttttt cgcgaggtca ggtcatcatc
     6241 gcccaaggtg gccgtcgccg ccaattgtac gagatcccct tgtcgatcga agacgatgca
     6301 gggaatgcca gcgatggcag cttcctcgat gatgcatttc gccaaacagg tttttccgga
     6361 accggatgtt ccaaagatgg cgagatgctt ttggatatcg gagggttcga gacgtgcggc
     6421 tttgcccgtt tccctctctt gccccaacca gatgggtaat ggagtgagag cggtttgggc
     6481 gcgcggtgac gcgctctcgc tgtttgcccg agaagccggt ggtggacggt gggagcgggc
     6541 gacagagccg cgacgaggta gttgttcgac atcaggtagt gttcgtcccc atctcgtcca
     6601 gcacggttgt tgcgcggggc aggttttgca aatcgtcgtg tccgcgggac cggtggcgtc
     6661 ttgttctcgc catcgttcga gctgtgaaac ccatgtaact aattgagttt gctgggtaat
     6721 atctggatgc gatacctcga gtctgcgcac gtcgtcgggt tgaatctgca ccaggacaat
     6781 cgacgatttc ccattccgcg cctttcggtc cagcgcgtat atcattccga gaacctcatg
     6841 ggatcggggt tcgggtcgtt ggaaagaagt attgactatc cacgggatag cggtgggcgc
     6901 atggcagcag gccatcgcca cggatccttc gaccacgaca ctacccaggt ccacgcgtag
     6961 ggttgggtcc gataaggata gcgttttgtc gatggcctcc tgcgcggatt gggcgaggcc
     7021 tgccgcggcg ccaagcagtt cagcgaaggt agcgcatgcg cttctcagta attgatccac
     7081 ctgatagagg gtgtcgccgt cgatttgtgg aagaagggga atcagtcgag gatagatgta
     7141 gtcataagcg tgtcgccaac aggcgcgggc gaggtctttc tccgaaggtg gtattgcggc
     7201 gagcgatgaa agaagcgaat cgtttcgatg ggcggttgtc accagatggt caagggtttg
     7261 ggagagtgcg tcgtgcagga cgacttgtcc ctcgtcttga acgggaacac cttctttttc
     7321 gaggatgaac aatcgagggc atcgtgcggc tcgaaggacc tgttcgagag gaaggagggt
     7381 catgctgttt ccaccattgc ctcggggcga agcatgacga tcggaatgcg tttccggtcg
     7441 agggcaacat gggcgaggcc gcgggatacg agcgcctcgg tggcttgatg gatggcttcc
     7501 gcgggggctt cgagttcgcg gacgagcttt ggcagcgaaa tgaaggctgc ccggttgcgc
     7561 agcagggcca gtatggtttc ttgccactgg cgtgtgtcgc cccgggagac accgtcatcg
     7621 gtgaaggcgg ctccgatcag gttgccgacg actcgatcga tggcatcaat atcgaacacc
     7681 gcacgtcgaa tctcttcttc tgcgagggga acgtcgccgg tggctgccgc attgagaagt
     7741 tgttcaaacg ccgccaaagg agcc
//
LOCUS       DAOSOR010000248         7749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9405_length_7749_cov_4.980244, whole genome shotgun sequence.
ACCESSION   DAOSOR010000248 DAOSOR010000000
VERSION     DAOSOR010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9405_length_7749_cov_4.980244"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PK710_13740"
     CDS             complement(<1..676)
                     /locus_tag="PK710_13740"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT10834.1"
                     /translation="MKPRLPEVIITHAVLLLMSTVVLYPVLWVLKLALTPGGQLGADP
                     NPLPLPDSFYLGNFGAFLFAKDFNGNPLFWYQLGNSLLISAVATLIGVLCALSAAYGF
                     SRFTFAGRETGLKLMLISQMFPAVVTAVPLLFILDTLGLFGTTAGLVLIYSTASVPFC
                     IWMLKGFFDTLPRDLEESARLDGAGHWIIFFKIMLPLVRPGIAVTALFSFMTAWNEFI
                     MASIFLD"
     gene            complement(673..2931)
                     /locus_tag="PK710_13745"
     CDS             complement(673..2931)
                     /locus_tag="PK710_13745"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT10835.1"
                     /translation="MSEGGKTRFGWGMLVLVALVIVLFRPVTQLLRPQPTGVKIIVWH
                     SQRGQEREVLEDLLRRFNEEHRGQVYVEPLGVPDNSFKDKLVRNIPRGSGPDLFIRPH
                     NELGEFHDEKILRVVERGSLPFAPDAYVDKLLDGLSIEGALVGYPLTFKGLFQFYNTQ
                     LCPHGPPRDASELEEWKQKLPSGVVPLAYDASNPFFHAPFFIGAGGELFGQDGKTFTL
                     FEEPGLKSYRWPGQWKRSGVLPPEPNYNEMVRLFQSGKAATIIVGPWYQPSGEIGASG
                     TWDIAPLFQIWGKPTGSFVTVEGVYVSARTRHPEQAAQVASFLAGAVGEKERFDRLGL
                     PPVTRAAYDKLDPSPSSSRQVAMVRVQRQSLEHGIVTPNSARMAAVWTPATDLLSASL
                     AGRDFDKAVGDARYMLARVDEPKPPAADWKPYGIVLVVLLAVGTALVAMRVRRGVHGP
                     EAMRAKLTGFHARAALPYLLPGLLAVALLVFVPLIVGAGLSLFTHEHGTFTFTGLQNF
                     GRILFPPLERAFQSRSFYFALAVTVLWTLANVVLHVAIGVMLALLLRPSWNRLRTLYR
                     LLLILPWAIPNYITALMWKGMFNAQVGAVNVLLSPFGFEGYNWFDKFLPAFTANLVTN
                     TWLGFPFMMVITLGALQSIPGELEEAAMLDGATRWQRFRLVIFPHVRPALLPAIILGS
                     VWTFNMFNIIYLVSGGEPNSQTDILISEAYRWAFERGQRFGYAAAYSVLIFIFLLFYS
                     RVTERLQKDGAS"
     gene            complement(2928..4082)
                     /locus_tag="PK710_13750"
     CDS             complement(2928..4082)
                     /locus_tag="PK710_13750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT10836.1"
                     /translation="MTYSLTFDRVGKRYDQTVVLSDVTFRTEPGEFVVLVGPSGCGKS
                     TLLRLVAGLETITDGEIRAGDRVLNDVAPQDRGVGMVFQSYALYPHMSVRDNLAFPLY
                     VKGVSAEEQKRAVQEVAGILGLEPLLDRLPRQLSGGQRQRVAIGRCLVRKPEIYCFDE
                     PLSNLDAALRAQIRVEIKALQRKLKKTTLYVTHDQVEAMTLADRIVVLNRGIVQQVGS
                     PEQLYTRPANRFVARFIGTPSMTLVRGKVQQGRFRSGQLVVPLGLEDGEVLVGVRVED
                     VCVLTKPGGSDAAVEESDSFSATIRAVEPVGESGYLHLSVQGIELEGDDSKGTEADSG
                     QSLLASVPGHAAFGYRVGSEVMIKIRSERCHAFHRTTGEALTLSASGEEP"
     gene            4196..5011
                     /locus_tag="PK710_13755"
     CDS             4196..5011
                     /locus_tag="PK710_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT10837.1"
                     /translation="MSEYANDEEDFRWSLTFDDSVREATRAIMGTDRRSVARSVSAIL
                     GMLGAFFLLLLPTVSGADSNPSPSAPHRTPFVVHFGDSFVQAGLQQNLRPRFQALGAR
                     YITEAKTSSWLATWASGQQVDNLYWGYRPELFLVTLGANDLLFQPAEKRAYLVREIVQ
                     KMRGTPCIWIGIPIWETAPTEFVEMIRRESAPCRYFDSNQITTRISRQADKRHPDAAG
                     GQFWADTFWAWYQTQRDPQRGPWALKPAPPEEHAPRPDPDPWNRNSAASSPLP"
     gene            5064..7025
                     /locus_tag="PK710_13760"
     CDS             5064..7025
                     /locus_tag="PK710_13760"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HOT10838.1"
                     /translation="MASESQWRWVDRNGVQRVLSFDTLADALRNGALGRDVPVWREGM
                     PAFMPAGWVPELENACTQAPLTTAADAAAVGEISPPTQASLEAFQRLINPQKSSKDPR
                     QSPTVPSGKSMRTAILGSQAPHVAARPLPPSPMAAPRKPRPHEKSNPLDPPSQASHES
                     TTANAPTPSSPPAQSSPPIDTPPATPLAIQHSGQRSSSEQIETSRTAMVPSTPIDPSS
                     PLISTETHPSHFDQTMPSNVMRAEFERLAQRRRLLRILFASLSAIVLLIGVLALIALR
                     TRNSKLPQPETAPTTALSVESIPDAAPAQSAVPLAASTTPEPSVSPSEKSEKACKFQG
                     IRKRLVVGASKDIPMEVWSDPSERLVAVGFASRNHTALGFVLDPKTLTPERSFTKQAS
                     LHLTRVVPLNAQGTITFETDEQAPAAAVLNPLTVACSPPARLGTFKGSLTIRRASDTI
                     PEILWKLPFGDTLEAMRALPIPGKGIAVVFRANHELWLGWISEDHKAVGDLQKIQGVG
                     EKTGTPSLGWNGRRVLVAFADLPSDQAPWAVRVAEAAFGDTNITSSPLKFNVEKQEVP
                     RISPAVLGLADGRWVLVFTEGSAGSRSVRLQTFDSDLQPLEEPCAISKTGDNAGQGLA
                     TVGKTGGVVVYLTSIGPFYEVWAAGIDCQ"
     gene            7067..7738
                     /locus_tag="PK710_13765"
     CDS             7067..7738
                     /locus_tag="PK710_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10839.1"
                     /translation="MLNLVGCLPRHPSPPPSIEETAVGQGERFETGLTVYDEYFSTVH
                     QLHGEVVNAERQETDAISTLASVLDLLPTAPAAQVLRKLRERLPTLPAMELVTHDPIQ
                     GKPPSATVRLVHRGWPKENVKSMMLVLEASANANLDIAWRMKEIPERCQRMSDVGKEL
                     IHTVEHDFAREPMERRDQIRREFEASFQILGGMAASAEEIHQRTQGFTKDLEQALTVS
                     GSGIE"
BASE COUNT         1799 a         2462 c         2039 g         1449 t
ORIGIN      
        1 cgtcgagaaa aatcgacgcc atgatgaatt cgttccacgc ggtcatgaac gaaaataggg
       61 ccgttaccgc gatgcccgga cggaccaacg gcagcatgat cttgaaaaag atgatccaat
      121 gaccagcgcc gtccaggcga gccgattctt cgagatcccg cggcaacgtg tcgaaaaatc
      181 ctttgagcat ccagatgcaa aagggaaccg aggccgtgga gtaaatgagc accaaccccg
      241 ccgtcgtgcc gaaaagcccg agcgtatcca gaatgaatag caacggaacc gccgtgacga
      301 ccgcgggaaa catctgggag atgagcatga gtttgagtcc cgtctccctg cccgcgaagg
      361 tgaaacgaga aaagccatag gcggcggata gcgcgcacaa cacaccgatg agcgtggcta
      421 ccgcgctgat caacagggag ttgccaagct ggtaccaaaa caggggattg ccattgaagt
      481 ccttggcaaa taggaaagct ccgaaatttc ccaggtaaaa ggaatccggc aggggaagcg
      541 gattggggtc ggcgccaagc tgacccccag gcgtcagcgc gagcttcagc acccataaaa
      601 ccggatacag caccacggtg gacatgagca gaagcacggc gtgggtgatg atcacttccg
      661 gcaagcgagg cttcacgacg caccatcctt ttgcaaacgt tcggtcacac ggctgtagaa
      721 cagcaagaaa atgaagatga gcaccgagta ggccgcggca tagccgaaac gctgcccgcg
      781 ctcgaacgcc caacgatacg cctcgctgat gaggatatcg gtctgtgagt tgggctctcc
      841 tccgctgacc aggtagatga tgttgaacat attgaaggtc catacggagc ccagaatgat
      901 cgcgggcagc aaagccggcc gtacatgagg gaaaatgacc aggcgaaaac gctgccatcg
      961 cgtggcgcca tcgagcatcg ccgcctcttc caactcacca gggatcgact gcagcgcgcc
     1021 cagggtaatc accatcatga aaggaaaacc gagccacgta ttggtcacca gatttgccgt
     1081 gaaggcggga agaaatttgt cgaaccagtt gtaaccctcg aatccaaaag gagaaagtag
     1141 cacattgacc gcgcccacct gcgcgttgaa catgcccttc cacatcaacg ccgtgatgta
     1201 attcggaatc gcccagggca agatcaacaa cagccgatac agcgttcgca atcgattcca
     1261 cgatgggcgc agcaacaacg ccagcatcac gccgatggcc acatgcaaca ccacattcgc
     1321 aagcgtccac aatacggtca ccgccagcgc gaagtaaaaa gaacgggatt gaaacgctcg
     1381 ttccaggggc ggaaatagaa ttcttccgaa gttttgcagg cccgtgaagg tgaacgtgcc
     1441 gtgctcatgc gtgaaaagag atagccccgc gccaacgatc agaggaacga atacgagcag
     1501 cgcgaccgcc aacaaccccg gcaacaagta gggcaaggcc gctcgcgcgt gaaagcccgt
     1561 aagcttcgcg cgcatcgctt cgggcccgtg aacaccacga cgaacccgca tcgccaccag
     1621 agccgtgccc accgccaaca acaccaccag cacgatccca taaggcttcc aatccgccgc
     1681 ggggggcttg ggctcatcga ctcgagccag catatatcgc gcgtctccta ccgccttgtc
     1741 gaagtcgcgc cccgccagac ttgccgaaag caagtccgtc gccggcgtcc ataccgccgc
     1801 cattcgagcg ctgttgggcg tcacaatgcc atgttcgaga gactgtcgct gcacccgaac
     1861 catagccact tgccgggagg aagagggcga aggatcgagc ttgtcgtagg ccgcccgcgt
     1921 gaccggcggc agcccgagcc gatcgaatcg ctctttttct cccaccgcgc cagcaagaaa
     1981 cgaggccacc tgagcggctt gttccggatg tcgcgtccgc gcggaaacgt atacgccttc
     2041 gaccgtaacg aaagaacccg tgggtttgcc ccaaatctga aacaacggcg cgatatccca
     2101 cgtgccggaa gcgccgatct cccccgatgg ctgataccag ggacctacga tgatggtggc
     2161 cgcttttccg gactgaaaca gacgaaccat ttcgttgtaa ttcggctccg gcggcaacac
     2221 cccggagcgc ttccactgcc ccggccatcg gtagcttttc aaccccggtt cttcgaaaag
     2281 cgtaaaggtc ttgccatcct gtccaaaaag ctctccgccc gctccgatga aaaacggtgc
     2341 atgaaaaaat ggattgctcg catcatacgc aagcggcacc accccgctgg gcaacttctg
     2401 tttccactct tccaattccg acgcatcccg aggcggtcca tgaggacata attgcgtgtt
     2461 ataaaactga aaaagtcctt tgaatgtaag gggatagcca accaacgccc cctcgatgga
     2521 tagcccgtcg agcagcttgt cgacataggc atccggcgca aatggcagcg acccacgctc
     2581 gaccacccgc aaaattttct cgtcgtgaaa ctcgcccagc tcgttatgag gccgaatgaa
     2641 caagtcggga ccagaaccgc gagggatgtt gcgaaccagt ttgtctttga aagaattatc
     2701 gggaacgcct aggggctcca cgtagacttg accgcgatgc tcttcgttga aacgccggag
     2761 caaatcctcc agcacctcgc gctcttgccc tcgctgcgaa tgccatacga tgatcttcac
     2821 accggtgggt tgcggccgga gaagttgggt caccgggcga aaaagcacga tcaccaacgc
     2881 cacgagcacc aacatgcccc acccgaagcg ggtcttgcct ccctcgctca cggctcctct
     2941 ccagacgcgc ttaacgtcaa tgcctcgccg gtcgtccgat gaaaagcatg gcagcgctcc
     3001 gaccgaattt tgatcatcac ctcactgccc acgcgataac cgaaagccgc atgcccaggc
     3061 acgctcgcta gcagactctg gccactatcc gcttccgtac ccttgctatc gtcgccttcc
     3121 aattcaattc cttgcacact caagtgcaag tatccgcttt cccccacggg ctccaccgct
     3181 cgaatcgtcg cggaaaaact atccgactct tccacggccg cgtcgctccc gcccggtttc
     3241 gtcaacacac agacgtcttc cacgcgaact ccgacaagga cttcgccgtc ttcgaggcca
     3301 aggggcacaa cgagctgccc actgcgaaag cgtccttgtt gcaccttgcc tcgcaccagg
     3361 gtcatggatg gggttccgat gaagcgcgca acgaaacgat tcgcgggtct ggtgtataac
     3421 tgctcgggcg atcccacctg ctgcacgatg cctcggttca acactacgat ccgatccgcg
     3481 agcgtcatgg cttcgacttg atcgtgggtg acgtataggg tcgttttttt gagtttgcgc
     3541 tgcaacgcct tgatttcgac gcgtatttgt gcgcgcaggg cggcatcgag attcgacaag
     3601 ggctcatcga aacagtagat ttcaggcttt cgtaccaagc atcgtccaat ggccacgcgc
     3661 tgtcgttgtc ctcccgaaag ctggcgcgga agacgatcga gcaacggttc gaggccaaga
     3721 atcccggcca cttcctgcac cgcccgtttc tgttcctcgg cggaaacccc tttgacgtaa
     3781 aggggaaacg ccaggttgtc ccgaacgctc atgtgcggat acagcgcgta actctgaaaa
     3841 accatcccca cgccacgatc ctgcggcgcc acgtcgttca gaacgcgatc gcccgcgcga
     3901 atctcgccgt cggtgatggt ctcgagtccc gccaccagac gcaacaacgt ggatttcccg
     3961 cagccggatg gccccacgag aacgacaaac tcgccaggtt ccgtgcgaaa agtcacgtcg
     4021 gataacacca ccgtttggtc gtatcttttt ccgactcgat cgaaagtgag gctgtaggtc
     4081 atggatcgga gtgatgggct ggtcgtggga gtggacgggc cttgctcctg cgacaagtga
     4141 atgggaacac cagaccccga tcagcgtcaa ttgtcaattc aggagtgagt tacgggtgag
     4201 cgaatatgcg aatgatgaag aggattttcg ttggagcttg accttcgacg attctgtgcg
     4261 agaggctaca cgagccatca tgggaacgga tcgaaggagc gtagctcgaa gtgtctcggc
     4321 aatccttggg atgcttggcg ctttttttct cttgctattg ccaaccgtat ccggcgctga
     4381 ttcgaaccca agccccagcg cgccgcatcg caccccattt gtcgtgcatt ttggggactc
     4441 tttcgtgcaa gcaggcctac aacagaacct tcgtccacgc ttccaggctc tgggagcccg
     4501 ctatatcacc gaagcaaaaa ccagctcctg gctcgccacc tgggcctccg gacaacaagt
     4561 agacaacttg tactggggct atcgtcccga actattcctc gtgaccttgg gggccaacga
     4621 tcttctgttt cagcccgccg aaaaacgcgc gtacctcgtg cgcgaaatcg ttcaaaaaat
     4681 gcggggaacc ccttgtatct ggatcggtat tcccatctgg gaaaccgcac ccaccgaatt
     4741 tgtcgaaatg attcgtcgag aatcagcccc ctgccgctat ttcgactcca atcagatcac
     4801 cacgcgcatc agccgacaag cggacaagcg tcatcccgac gccgccgggg gacagttttg
     4861 ggcggacacg ttttgggcgt ggtaccaaac ccaacgggac ccgcagcgag gaccgtgggc
     4921 gctgaaacct gccccgccgg aagaacatgc tcctcgcccc gatcccgacc cttggaaccg
     4981 aaattccgcg gcttccagcc cccttccttg agaaaactca gactattgcc cctccctggg
     5041 ccgcgtccgc taagatacgc atcgtggctt ctgaatcgca atggcgctgg gtcgaccgaa
     5101 acggagtgca gcgagtcctg tccttcgaca ccctcgctga tgccttgcgc aacggcgctt
     5161 tgggtagaga tgtgccggtt tggcgcgagg gaatgcccgc attcatgccc gccggatggg
     5221 tgcctgaact cgaaaacgcc tgcacccaag ctcccttgac cactgctgcc gacgccgcag
     5281 ccgtgggcga aatctcccca cccacccagg cctccctgga agcctttcaa cgtctcatca
     5341 atccccaaaa atcgtcgaaa gatccgcgac aatcccccac ggtcccctcc ggcaaaagca
     5401 tgagaaccgc catcctcgga tcgcaagctc cccatgtcgc ggcccgaccc ttgcccccaa
     5461 gccccatggc ggcaccccga aaacctcggc cccacgagaa atccaaccca ctcgaccctc
     5521 cgagccaagc ctcccacgaa agcaccacgg ccaacgctcc tacaccatct tctccaccag
     5581 cccaatcctc ccctcccatc gatacgcccc ctgccacacc cctcgcaata caacacagcg
     5641 gtcaacgaag ttcctcggag caaatcgaaa cgtcccgcac agccatggtc ccttccacgc
     5701 ctattgaccc ttcttcccca cttatcagca cggaaactca cccttcccac ttcgaccaaa
     5761 ccatgccgtc caacgtcatg cgcgccgaat tcgaacgcct cgcccaacga cgccgcctgc
     5821 ttcgtatcct cttcgctagc ctctccgcca tcgtgttgct catcggtgta ctggccctca
     5881 tcgctttgcg gacgcgcaac agcaaattac cacagccaga aaccgcccca accaccgctc
     5941 tatccgttga aagcatcccc gacgccgcac cagcgcaaag cgcggttcct ttggcagctt
     6001 ccaccacccc cgaacccagc gtctcccctt ccgaaaaatc agaaaaagct tgtaaatttc
     6061 aagggatacg aaaacgactc gtcgtgggag cttcgaaaga tatccccatg gaagtctggt
     6121 ccgatccctc ggaacggctc gtcgctgtcg gtttcgcctc tcgaaaccat acagccctcg
     6181 gcttcgtcct cgacccgaaa acgttgaccc cggaacgctc ctttaccaag caagcttccc
     6241 ttcacttgac gagggtggtt ccgctgaacg cgcaaggaac catcacgttc gaaaccgacg
     6301 aacaagcccc tgcggctgcc gtcctgaatc cgctcaccgt tgcctgttcg ccaccggcga
     6361 gactgggcac gttcaaaggt tccctcacga tacggcgcgc gtcggacacc atccccgaaa
     6421 tcctctggaa acttcctttc ggggacacgc tcgaagccat gcgcgcgcta cccatccctg
     6481 gcaaaggcat cgcggtggta tttcgagcca accacgaatt atggttggga tggatctccg
     6541 aagaccataa agccgtcggc gatctgcaaa aaatccaagg ggtcggcgaa aaaaccggaa
     6601 ccccatcgct cggatggaat ggccgacgcg tgctcgttgc cttcgcggac ttgccctctg
     6661 accaggcccc atgggctgtg cgcgtggcgg aagctgcttt cggcgatacg aatatcactt
     6721 cgtcgccact gaaatttaat gttgaaaaac aagaggttcc aagaatttcc cccgctgttc
     6781 tcgggctggc cgacggtcgc tgggtgcttg tatttaccga aggatctgct ggctcccgaa
     6841 gcgttcgcct gcaaaccttc gacagtgatt tgcagcctct cgaagagcct tgtgccattt
     6901 cgaaaaccgg cgacaatgcc ggacagggcc tggcgactgt tggaaaaacg ggtggagtcg
     6961 tggtgtactt gacctcgatc ggtccgtttt acgaggtatg ggccgcggga atcgattgcc
     7021 aatgatcaaa atggccccat cgcgccgttg gtttttgttt ctatgtatgt tgaacctcgt
     7081 cggctgcttg ccacggcatc cgtcgccccc tccttctatc gaagaaacgg cggtcggaca
     7141 gggagagcgt ttcgaaaccg gacttacggt ttacgatgag tacttcagca cggttcacca
     7201 acttcatggg gaggtcgtga acgctgaacg gcaagaaacc gacgcgattt ctacgctagc
     7261 gtcggtgttg gatctattgc cgacagcccc cgcggcgcag gtgttgcgaa agctgagaga
     7321 acgtttgccc acgctgccgg ccatggaatt ggtcactcat gatccaatcc aaggaaaacc
     7381 tccctcagcc accgtacggc tcgtccaccg agggtggccc aaagaaaacg tcaaaagcat
     7441 gatgcttgtg ctcgaggcca gcgcaaacgc gaacctcgac atcgcctggc gcatgaaaga
     7501 gattcccgaa cgctgccaac gcatgagcga tgtgggcaag gaactcatcc acacggtcga
     7561 gcacgatttc gcgcgtgaac cgatggagcg acgcgatcag attcgacgag agttcgaggc
     7621 ttcctttcaa attctcgggg ggatggctgc ctcggcggag gaaattcatc aacgaacaca
     7681 agggtttacg aaggatttgg agcaagcgct gacggtgagc ggttcgggga tcgaatgaac
     7741 gtcaaccgc
//
LOCUS       DAOSOR010000249         7749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9408_length_7749_cov_3.385755, whole genome shotgun sequence.
ACCESSION   DAOSOR010000249 DAOSOR010000000
VERSION     DAOSOR010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9408_length_7749_cov_3.385755"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="PK710_13770"
     CDS             <1..1009
                     /locus_tag="PK710_13770"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT10840.1"
                     /translation="IILDDVSVRASNVLGNVGRGFKVAMEVLNAGRLSLASGCLGSCK
                     RLIRLTSERAQERHAFGRRIAEFELIKEKIAWMMGETWALESMTYLTAGLVDAHVSDY
                     SLESAICKIFGSETLWRIASEALQVAGGAGYMASLPFERMLRDARVNSIFEGTNEILR
                     LYIAQSGMQAPARAMSEVNHAIRQPIKGFGLLSTFAVQKVRSSFGRKRLGRVHPLLAH
                     EAEKFDSFSAELAKLVEQTIRSHGKELKERQLLQRRLANMAIDLYALAACLYRTTRDI
                     ERFGEQGAALALSLTHAFSQDAERRLTHNLVGMNKNRDTDDMNIAAQALAAGKYPFDV
                     I"
     gene            1437..1889
                     /locus_tag="PK710_13775"
     CDS             1437..1889
                     /locus_tag="PK710_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOT10841.1"
                     /translation="MLRRWSDVWNDFDQTLAWMEGVRRQMDRTFSGPALGLDLRASRG
                     FPCTNLVDEGNEYILRAEVPGLSEKDIKVTVHQDVLTLSGARESEAPEGYTTHRRERQ
                     PLRFSRSFSFPSRVDLEKVKASVKNGVLTVHLAKVEEAQPRQIAVTTE"
     gene            1919..2305
                     /locus_tag="PK710_13780"
     CDS             1919..2305
                     /locus_tag="PK710_13780"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOT10842.1"
                     /translation="MTTESNLTKRQETQPEVTRSERLETPPVDIFENQNEILLVADMP
                     GVTKEDLQVRVDKGVLVLEGKKTFEMPKGLLNCETEPCMYTRSFSLPETIDGDRISAK
                     LELGVLTVHLPKRESVKPRQIQVTGG"
     gene            2422..2640
                     /locus_tag="PK710_13785"
     CDS             2422..2640
                     /locus_tag="PK710_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10843.1"
                     /translation="MTFEEAIFWLDEQGGRWSTHASGSTVQVIVSLGGHQVQAPVERL
                     LAEQVRQAFIQAVQAIRSTVSHGRSRRT"
     gene            2656..3576
                     /locus_tag="PK710_13790"
     CDS             2656..3576
                     /locus_tag="PK710_13790"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOT10844.1"
                     /translation="MIPKVGLQLLSPHLVRCGTLYVAMGIFLSGLGLVSCSHEPHAPP
                     ETKNTPRLIASGASAELPVVFQHQVAPLPRWSHQGVPDDALQAIVVTSPSWKSYHGQL
                     RRFERQDYTRAWNRADAGAVAVSLGRYGLGWGRGLHPEQKGGPVKGEGDHRSPAGIFD
                     LGEARGFAKELPPGTTWPFQQSANRSRCMDNPRSIAYNTFISTEAMLLPVSVGVARRE
                     TVFEMMLFVMHNTSPVLRGAGSCVFLHVWAKPESPTQGCVAMSRESLQRILPWLDLER
                     RPVLVQLPEHEYRRFEKDWSLPTLDPEDSG"
     gene            complement(3609..5651)
                     /locus_tag="PK710_13795"
     CDS             complement(3609..5651)
                     /locus_tag="PK710_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10845.1"
                     /translation="MKPFNRSSHRPSPLVNATDPAVWCLFLTTLYVSLTATSFARLYH
                     HNVIDDAYISFQYARNLVLGQGLVFNPGQYVEGYTNFLWVVVLAPFYALSQSLGTDFV
                     RMAIGISVALSMLNLALLFAIARHLFPRDWFATSVTIVLCGVDNAYLGYAISGLENHL
                     LIFLSLTCLWAWLRTHKLRGLWVGLLLAMTCMTRPDAGLFVFAFVLATIPSLLWKKPS
                     PPQIPQPATLAQIGLALGTFIIVFGLYFGWRVHYYDSWLPNTYYLKIGNEINAIERGW
                     HYLRTFAEDRFYIPALALLALPWLRHTVVRWLLFWLLLHAAYVVYVGGDFYSGHRFLL
                     VLLPAIALLIGGLIHHGRKRISSTPLAHWFMAHPPWMAFIIGITHGTVALGFCLLSAR
                     GFERGPYTHEISLYGEQVHNNIKFSKWLGSYSHAGESIVAGDIGATGFFTNLIVHDVY
                     GVIDPAIARMKVNNFGQGKAGHEKRGSRDYLLSHHPTYVKWGYVPGDLRPYGYYVFTD
                     FPPGFQQPGLWVREDLGEGSLLHDPNTRLDLHNLSGWRQSGNAFENAPTLRPVAGQEH
                     VFGHSGPYLNSFTESLGDRATGTITSPPFELQGDLLLLWVGGGRDPQRLRVSLIVEGT
                     RVASATGHNHEVLGRRAWNITSFKGKTAILEVVDDSPDVWGHIMVGGIRQWLKAKP"
     gene            5731..6552
                     /locus_tag="PK710_13800"
     CDS             5731..6552
                     /locus_tag="PK710_13800"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOT10846.1"
                     /translation="MPTVSKSVTEQQARVGELSESLEDYLEIILQLQQEKRVARVRDI
                     AAKKGVSNASVSNALHRLSRDGFVNHQAHEFVELTDLGLDLAHRILRRHEFLVSFLRD
                     VLQVSETGAERDACALEHHLSSETLEALSTFYQFVCSCPRVGVGFLQAYKACCTRKEG
                     ATDVGEGLGCANAGPEGSRRCEAGGKEEDDLLPLSELGAGHVGLVVRLKAPRLLRKRL
                     VDAGFLPNVEVEVRRSDLEHRGVQLVVQGCEMLMDAQEAAVVLVRPIAPKKNDLA"
     gene            6606..>7749
                     /locus_tag="PK710_13805"
     CDS             6606..>7749
                     /locus_tag="PK710_13805"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOT10847.1"
                     /translation="MSDRQIVSSKTFCRRRLSWRTFGMVGATLVAMTILLWIAIHRVP
                     WLGPWLADGVRAVVGPAPVAWAEDVAYGWQDRVNRWRHGKDAPHTYWEVPGDWVPVAS
                     ASPVPAAAESSPPCFPPKNFEPPFRVVAASGDGVWVPVDGRASVGGQSVMFKTMVHPD
                     HERPYTVLALVAMDVSKVRIHAVAGTLEPRSTTFPESLRTGIVPEAQRPSLIAAFNGG
                     FQTIQGGYAMMVDGHRIGEPRADACTVGLYRDGTIRIRSWPDLEAETDRMTAYRQTPR
                     CLVENGQRHPELENRYNTSWGAVVDGGTVIRRSALGVSKDGNVLYFGMGDSLTARSLA
                     DGLKAAGAHDVAQLDVNHVFPRFVFFAEGARGLEAVAPLCPGFSFSP"
BASE COUNT         1669 a         1924 c         2405 g         1751 t
ORIGIN      
        1 catcatcctc gatgacgtat cggtgcgggc gagcaacgtg ctaggaaacg tggggcgcgg
       61 tttcaaagtc gcgatggaag tgctcaatgc aggacggctt tctctggctt ctggatgtct
      121 gggaagctgc aaaagactca tccgattgac cagtgaacga gcgcaggaac gacatgcgtt
      181 cgggcggcgc atcgccgagt ttgaactcat caaagaaaaa atcgcgtgga tgatgggaga
      241 gacctgggcg ttggagtcaa tgacatacct gaccgctggg ctcgtggatg cgcacgtctc
      301 ggactattcc ctcgaaagcg ccatttgtaa gatattcggc tccgaaaccc tgtggcgaat
      361 agccagcgaa gcgctgcaag tggccggagg agctggatat atggcttctc ttcctttcga
      421 aaggatgctt cgggatgcgc gggtaaactc gatcttcgaa ggaaccaacg aaattcttcg
      481 gctgtatatc gcccagagcg gcatgcaagc gccagcccgt gccatgagcg aggtgaatca
      541 tgccattcgt cagcccatca aaggattcgg cttgttgagt accttcgcgg tgcaaaaagt
      601 ccgctcttct ttcggccgga aacgattggg acgcgtacac ccgctgctcg ctcacgaagc
      661 cgaaaaattc gattccttct ccgccgaact cgccaaactc gtcgaacaaa ccattcgttc
      721 ccacggaaaa gaactcaaag aacggcagct tcttcagcga cgattggcca atatggccat
      781 cgatctttat gcccttgccg cctgtttgta ccgaaccaca cgtgatatcg aacggtttgg
      841 tgagcaagga gcggctctcg ccctctcgct gacccacgca ttctcccaag atgccgaacg
      901 tcgtctcacg cacaaccttg tgggaatgaa caaaaaccgg gataccgacg acatgaatat
      961 cgcggcacag gctcttgccg cgggaaagta cccctttgat gtcatttgat ggcggtcggg
     1021 gatacttcgg gtaacgggag cgccactgtt ttcgcgacaa cgaaaccttt tccttgcagc
     1081 gctgcaatcc tacaaaacgg agtctgttcg aactgcttga cttctctatg gattcagttt
     1141 ggcaccattc atgcttacta tttcgggtgc agcgtcggac gcgacaagcc ttgtccctga
     1201 cgttgtccca tcttctcaac gcccggtaga cgcatagccc ccctcgtccc gggcgttgct
     1261 ccttttgtcc tcctccacga gccgctgcaa ggaacgtgcg caacaaccat tgacaccagt
     1321 tttcttgtgc ataccttgtc aatcaccgat tggttatcca aggggcattc accgaagcat
     1381 cgtcgcgggt agcacgattg ccggtcagcc cgtttcccca atcatggagg tgcatcatgt
     1441 tgcgtcgatg gagtgacgta tggaacgatt tcgatcagac tcttgcatgg atggaagggg
     1501 ttcgacgcca aatggaccgg acgttttcgg gaccggcgct tgggctcgac ctgcgcgcat
     1561 cccgtggctt tccatgcacc aatttagtgg atgagggcaa cgaatacatc cttcgcgccg
     1621 aggtgcccgg cttgtcggaa aaggatatca aagtcaccgt tcaccaagat gtgcttaccc
     1681 tatccggtgc ccgagaaagc gaagcgcccg agggatatac gacccatcgt agagagagac
     1741 agccccttcg gttttcacga tctttctcct ttccgtcgcg tgtcgacctc gagaaggtca
     1801 aggcatcggt caaaaacggc gtgttgaccg tgcatcttgc caaggtggag gaggcccaac
     1861 ccaggcaaat cgccgtgaca accgaatgag tagaagggtt tagaaaggag tgattgccat
     1921 gacaacggaa agcaacttga cgaaacggca agaaacacaa ccggaagtca cgcgttcgga
     1981 gagattggaa acgcctccgg tcgacatttt cgagaaccaa aacgagattc tcctggtggc
     2041 tgacatgcca ggggtgacga aagaagacct gcaggttcga gtcgacaagg gagttctcgt
     2101 cctggaaggg aaaaagacct ttgaaatgcc gaaagggctg ctcaattgcg aaacggagcc
     2161 ttgcatgtac actcgctctt tttcgctgcc cgaaaccatt gatggagaca ggatttcggc
     2221 aaaactagag cttggtgtgc ttacggtcca ccttcccaaa cgtgagtccg ttaagcccag
     2281 gcaaattcag gtcacggggg gctgaaggct cgggaagcgt tttgtgaaat cgtcgtcggc
     2341 ccgggaacaa tatccgcacc cttcctgttg cttcccacat tctgagccgg ctttgcagtc
     2401 ggtttggaat gtgagggagc catgaccttc gaagaagcga ttttctggct ggatgaacaa
     2461 ggaggacgat ggtcgaccca tgcctccggt tccacggtgc aagtcatcgt ctcgctgggt
     2521 gggcaccagg tccaagcacc ggtggaacga cttctggccg aacaggttcg tcaggctttc
     2581 attcaagccg ttcaagcgat tcgctccacg gtatctcatg gaaggtctcg tcgaacttga
     2641 ccttttctgc ttaacgtgat tcccaaggtt ggattgcaat tactctctcc tcatctcgtt
     2701 cgctgtggga ccctttatgt ggcgatgggg atctttttgt ctggtttggg gctcgtgtct
     2761 tgttctcatg agcctcatgc gccacctgaa accaaaaaca ccccccgctt gatagcctcc
     2821 ggggcttctg ccgaactgcc cgtggttttc cagcatcagg tagctccctt gcccagatgg
     2881 tcacaccaag gcgttccaga cgatgcgctg caggcgattg tcgtgacgag cccctcgtgg
     2941 aaaagctatc atgggcaatt gcgacggttc gagcgtcagg actatacgag ggcgtggaat
     3001 cgtgcggatg cgggtgctgt tgccgtttcc cttgggcgat acgggcttgg atggggaagg
     3061 gggcttcacc cggagcaaaa aggtgggccg gtcaagggcg agggggatca tcgttccccc
     3121 gcgggcattt tcgacctcgg cgaagcacga gggttcgcca aagaattgcc tcccggcacg
     3181 acgtggccgt ttcaacagtc ggcaaaccgt tctcggtgca tggataaccc aaggtccatc
     3241 gcgtacaata cgttcatttc caccgaggcg atgcttttgc ccgtctccgt gggcgttgcc
     3301 cgacgcgaga cggtattcga aatgatgcta ttcgtgatgc acaacacctc cccggtgctt
     3361 cggggggcgg gttcttgtgt gtttttgcat gtttgggcga agcccgagag cccgacccaa
     3421 ggttgtgtag cgatgtcgcg ggagagcctc caaaggattc taccttggct ggatttggaa
     3481 cgacgacccg tattggtgca acttccggag catgagtacc ggaggttcga aaaggattgg
     3541 tcgttaccta ctttggatcc ggaagatagt ggttaagcct gctggctcac gcgcgacggg
     3601 ctcacgtttc atggctttgc tttgagccat tggcgaattc cgcctaccat gatgtgtccc
     3661 caaacgtccg ggctatcgtc gaccacctcg agaatcgcgg tttttccttt gaaagaagtg
     3721 atgttccagg ctctacgccc caggacttca tggttgtgtc cggtggcgga tgccacacgg
     3781 gtaccttcga cgatcaacga tacgcgaagg cgttgtgggt ctcttcctcc gcctacccaa
     3841 aggagcaaca agtctccttg aagctcgaag ggtggagagg tgatggttcc ggtggctcgg
     3901 tcgcccagcg attcggtaaa cgagttcagg taggggcctg aatgtccaaa gacgtgctct
     3961 tgtccggcga cgggtcgcaa ggttggggcg ttttcaaagg cgtttccgga ttgtctccat
     4021 ccgctgagat tgtgtaagtc caggcgtgtg ttggggtcgt ggagcaagga accttcaccg
     4081 agatcctcgc gaacccacag cccaggctgt tgaaatcccg gcgggaaatc ggtgaagaca
     4141 taatagccat agggacggag atcacctggg acatagcccc atttcacata ggtggggtgg
     4201 tgggaaagca ggtaatcgcg cgagccgcgt ttctcatggc ctgcttttcc ttggccgaaa
     4261 ttatttactt tcatgcgagc aatcgcgggg tcgatcactc cgtagacgtc gtggacgatc
     4321 agattggtga aaaagcccgt ggcaccgata tctcctgcca cgatgctttc cccggcgtgg
     4381 gaataagagc ccaaccactt ggaaaatttg atgttattgt ggacttgctc gccgtataga
     4441 gagatttcgt gggtgtaagg gcctcgttcg aagccgcggg cgctgagcag gcaaaaacct
     4501 aaggcaacgg tgccgtgggt gatgccgatg atgaatgcca tccagggggg atgtgccatg
     4561 aaccagtggg cgagtggtgt ggaggagatg cgttttcgtc catggtgaat gagccctccg
     4621 atcaaaaggg cgatggcggg aaggagcacc agaaggaatc gatggccgct gtagaagtcg
     4681 ccgcccacgt acacgacata agcggcatga aggagaagcc aaaaaagtag ccatcgcacg
     4741 acggtatgac ggagccaggg caaggcgagc agggcgaggg cagggatata gaatcggtct
     4801 tcggcgaacg tgcggaggta gtgccagcct ctttctatcg cattgatttc attgcctatt
     4861 tttaaataat aggtgttggg aagccaagaa tcataatagt gaactcgcca tccgaagtag
     4921 agtccgaaaa cgatgatgaa ggtgccaagg gccaatccga tttgggccaa ggtagcgggt
     4981 tgcggtatct ggggtggcga gggttttttc caaagcaggg atgggatagt ggccaaaacg
     5041 aaggcgaata cgaacaatcc tgcatcgggg cgagtcatgc aggtcatggc aagcaaaagg
     5101 cctacccaaa gtccacggag cttatgggtg cggagccatg cccacaggca agtgagggaa
     5161 agaaagatca gcaggtggtt ttcgagaccg ctgatggcat acccgaggta ggcattgtcc
     5221 acgccgcaaa gtacaatggt gacggaggta gcgaaccaat cgcgagggaa aagatgccga
     5281 gcgatggcga aaaggagcgc aaggttgagc atggaaaggg caacggaaat gccgatggcc
     5341 atgcgaacga agtcggttcc gaggctttgc gacagggcgt agaagggggc tagcaccacg
     5401 acccatagaa agttggtata accttccacg tactgtcctg ggttgaacac caatccctgg
     5461 cctagcacga ggttgcgggc gtattggaac gagatatagg cgtcatcgat gacattgtga
     5521 tggtagaggc gggcgaacga tgttgcggtc agggatacgt acagggtggt gagaaatagg
     5581 caccacacgg ccgggtctgt ggcattgaca aggggtgagg gtcgatggga ggagcggttg
     5641 aagggtttca tgatcgacgc aatcggccgt gctgcgagaa gtactagtat ttcccttgac
     5701 ttacatttgc ctggcctata cttgttagca atgcctaccg tttccaaatc tgtcacagaa
     5761 cagcaagccc gagtaggcga gctatccgaa agtctcgaag attatctcga gattatactt
     5821 caacttcagc aggagaagcg agtcgcgcgg gttcgtgaca ttgctgccaa aaaaggggtg
     5881 tcgaacgcga gtgtatccaa cgcgttgcac cggctttcgc gcgacggatt tgtgaatcat
     5941 caggcgcatg agttcgtgga gctgacggac ttgggtttgg atttggcgca tcggatttta
     6001 cgtcgacacg agtttttagt ctctttttta cgtgatgtgt tgcaggtttc ggagacggga
     6061 gcggagcggg atgcgtgtgc gctcgagcat catctttctt ccgagactct cgaggcgctt
     6121 tcgactttct accagtttgt atgttcttgt cctcgggtgg gagttggttt tttgcaggcc
     6181 tacaaggcat gttgcacgcg caaggagggt gccacggacg tgggggaggg gctgggttgt
     6241 gccaacgcgg ggccggaagg ttcccgccgt tgtgaagctg ggggaaagga ggaagatgat
     6301 ttgcttccgt tgagcgaatt aggggcgggg catgtggggc tggtggttcg tctcaaagcg
     6361 ccccgattgc ttcgaaaacg cttagtggat gcaggttttc ttcccaacgt ggaggtggag
     6421 gttcggcgtt ccgatctgga gcatcgaggg gttcaactcg tggtgcaggg ctgtgagatg
     6481 ctcatggacg cgcaagaggc ggcggtggtt cttgttcgtc ccatcgcacc gaaaaaaaac
     6541 gatttggcgt gacggcgtgg ggttgtctcg acgtcggggg ttgtgcgcag taggcttgcg
     6601 tagttgtgag tgaccgtcag atcgtatcgt ccaaaacgtt ttgtcgccgt cggctatcgt
     6661 ggcgtacgtt cggcatggtg ggagcgactt tggtcgcgat gacgatattg ctgtggatag
     6721 cgattcaccg cgtgccatgg ttgggcccgt ggttggccga cggtgttcgg gctgtggtgg
     6781 gtccagcgcc cgtggcatgg gcagaggatg tagcgtacgg ctggcaagat cgcgtgaatc
     6841 ggtggcgaca cggtaaggat gcgccgcata cgtattggga agtcccaggc gattgggtgc
     6901 cggtagcttc ggcgagtcct gtgccagcgg ctgccgaatc ttcgcctcct tgctttccgc
     6961 caaaaaactt cgaaccgccg tttcgtgtcg ttgcggcttc aggggatggc gtgtgggtac
     7021 cggtcgatgg tcgggcgagt gtcggaggac aatccgtgat gttcaaaacg atggttcatc
     7081 ccgaccatga gcgcccctat accgttctgg cgctggttgc gatggatgtg agcaaggtcc
     7141 gaattcatgc ggtggcgggc actttggagc cgagaagtac gacgtttccc gagtcgctgc
     7201 gaacagggat cgttcccgaa gcgcagcggc cttctctcat cgcggcattc aatggcggtt
     7261 ttcagactat ccaaggggga tatgccatga tggtggatgg gcatcgaatc ggtgagcctc
     7321 gtgctgatgc atgcaccgtg ggactttatc gagacggcac gattcggatt cgttcgtggc
     7381 cggacctaga ggccgagacg gatcgcatga ccgcatatcg tcaaactcct cggtgtcttg
     7441 tcgaaaatgg gcagcgtcat cccgaattgg agaaccgata caatacgtcg tggggtgcgg
     7501 tagtcgatgg gggaacggtc attcgacggt ctgcgttggg tgtgagtaag gatggaaacg
     7561 tgttgtattt cgggatgggg gattcactga ccgcccgttc gcttgcggat ggtctgaaag
     7621 ctgcgggtgc gcatgacgtg gcgcagctcg atgtcaatca tgtgtttcct cgcttcgtgt
     7681 tttttgccga aggcgcgcga gggctggaag cagtggcgcc tttatgtccg ggattcagtt
     7741 tctccccgg
//
LOCUS       DAOSOR010000250         7740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9413_length_7740_cov_4.823162, whole genome shotgun sequence.
ACCESSION   DAOSOR010000250 DAOSOR010000000
VERSION     DAOSOR010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7740
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9413_length_7740_cov_4.823162"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="PK710_13810"
     CDS             complement(<1..810)
                     /locus_tag="PK710_13810"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT10848.1"
                     /translation="MSQLLRMTAPLQEWIQDARRAFARRDYHQALMLLNRAAAVVIEE
                     ELGEEYLVPILRAVGDARCGLGDWQGAQDHYREALERMEPLGLAESADAAGLLCNLGY
                     CLDQQQRFEDSEPLYVRALKLYVRNFGNDHPILVSVLTNMAFRLTMLDDLAGARGLFE
                     RALDIMLRYVDAHHLALVEPLNNLGEVVRRQGDAELAAELLLRAVGILEQKVPDDGHE
                     DLDVLLRNAARALGDAGRQEEAMALLQRNIRCAGAGCSVVMERLPDACLQDE"
     gene            complement(878..1921)
                     /locus_tag="PK710_13815"
     CDS             complement(878..1921)
                     /locus_tag="PK710_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10849.1"
                     /translation="MSLKSWMESYSIWAFAFGYFACYVPYSLITKMLTKGLVPGSEGQ
                     AISGFAVLPISAAAAAVGMVIFVSAMRWWKYANHVRVLGVNIPTPSRWTLLSGLCATC
                     TVGTTTLAYTFEGVSIVFAMLLMRGGVLVIAPIVDAISGRKVRWFAWAGLGMSILALL
                     VSLVDTKSYAMPFICVIDIIVYLASYFVRLQFMSRLAKSDDADTNRRYFVEEQMVATP
                     ALAVILAIYALLGSGNLAEELSKGFTAYWGQSIAIAMVIVGFLSQGTGVFGNLIYLDR
                     RENTYCVPVNRSSSILAGVVASYLLLIFPGQRAPGASELIGAALVIGAIVFLTIPPMM
                     EKRRQRAQQAAST"
     gene            2474..4543
                     /locus_tag="PK710_13820"
     CDS             2474..4543
                     /locus_tag="PK710_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10850.1"
                     /translation="MHSLRLRTVLGLGLLGIAACTPASLPPSSTHPTVVATPPSALPP
                     DLSPVKDPNSIVLVARLRNLQDSLGTMERMFRLPISLTDELQKVVAERAGDALQMDAG
                     VDVAVALSPASTDTDPKFFGAVSIPMANLEGALSQLEKRGNVVHLAPGVYRAKFERPQ
                     SEDGESSDSPTSESETPKEYRCDLAASLGNAPARLVCSEDEQALDSLRPWLTRGLPLQ
                     PTAKEDLEVHLRFAPLRERYVPVARTHASDYATTAKQWMETILNIRDPKLLMLPERVM
                     HESITFVEDMQEASFFGDFIPETNEISTRVTIAFRSRNSWFTQVYTDANGRSGPPPEI
                     FWRLPKDADNATYASWVDPAHFEGFNDTARSLMSHALELTPLSAQSKSAIDTFIASIP
                     LHKTVAVSARGEARRTTSAGNVTTARDAVREFEQFFQSIIGWTILGMEAPSAPYADHF
                     KKGVATMEQLMHEARKDAHLGPEMRKATWIPQIRWRSPFPGYPQGTTGIEITASFDSK
                     DIWSFANTKPGTPHPPGAAARGSITLHGVVIPNGPNRTWIGISADPKTLQEKSRVVVS
                     GPSQNTLASLADLEPLRQSSTIGGGFIIYGSMVDNMMKTFPQNPSVDMRLVHQSLETM
                     PNQLRTPVLQLHTATAGDTPTNTIELRLQRGTVDDLANLIGFVLSPQGQQLLEQLRKT
                     DIPLDVP"
     gene            complement(4615..5112)
                     /locus_tag="PK710_13825"
     CDS             complement(4615..5112)
                     /locus_tag="PK710_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10851.1"
                     /translation="MSHDDSSDMPAWARYLLLGVFFLLVLPIAIYGMYRQIFVAQEIA
                     PRIQKDLLDPYQAALAQGDFEKARSTYTTTEYQQRVSVEEYRQGIEKFRNEFGEISLL
                     SLHECTETKEPGRPWFQRCVVHYHTSKGVVAVSFEVVEQKGQYLIDRTFRYGPAGQSR
                     VEAVL"
     gene            complement(5161..7101)
                     /gene="treZ"
                     /locus_tag="PK710_13830"
     CDS             complement(5161..7101)
                     /gene="treZ"
                     /locus_tag="PK710_13830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HOT10852.1"
                     /translation="MYPLGDPSLGAVYLGNQRCLFRVWAPFVARIQVHLHEPDGRRVG
                     LQPRPFGYHQAVVEGVAPGTRYSYLLDDAIERPDPASRCQPEGVHGPSEVVDPSFPWT
                     DTGWCGLQLEDYILYEMHVGCFTPEGTFDAAIAQLDSLVDLGITAVELMPVAQFSGER
                     NWGYDGVYPYAVQHSYGGANGLKRFVDACHQRSMAVVLDVVYNHFGPEGCYVQDFGPY
                     FTDQVRIPWGSAINFDGPHCREVRRFFVENALHWVSDYHIDALRIDAVHAIKDFSARP
                     FLEELAEALHRRGKSLGRRVYCIAESDLNDPRLVLPSSQGGYDMDAQWSADFHHALHV
                     LLTGEQEGYYEDFGSFSQLVRAVQEGWAYAGDYSAYRKRPHGRGPRDIRAGQLIVYCQ
                     NHDMVGNRRAGERLAALVSFEALKLAAGAVLLAPYIPLLFMGEEYGEIAPFQYFVHHG
                     DPSLIDAVRRGRKEEFATFRWQQEPPDPQEEETFLRSKCDLGLRDRQEHEVLFRLYRQ
                     LIHLRKTVPALRVLDKRRTYVRGFEEERVLFIRRWVHPYDQDDRDRRIGDRNAEGREV
                     IIAMNVSGASVTRSLPFSSGRWRLLMDSASSLWACPSESSVRAEHGGGPASVRDQFVS
                     QGEVTVSLRPYAFAVFSRVEET"
     gene            complement(7223..>7740)
                     /locus_tag="PK710_13835"
     CDS             complement(7223..>7740)
                     /locus_tag="PK710_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HOT10853.1"
                     /translation="GRPAFCIRAVLAATLSSCYGIYNGFELCENRAIPGREEYLDSEK
                     YEYKVWDWDRPGHIKPLITKLNQARHQHPALQEYDNLRFHAADSNDILVYSKATPKRD
                     NVILAVVNLNPFEVRESLVHVNPWELGKGDNEPYGVRDLLSGQRWTWQGWTNYVRLDP
                     RHQPAHLFVIE"
BASE COUNT         1838 a         2286 c         2056 g         1560 t
ORIGIN      
        1 ctcgtcttgc aagcatgcat cgggcaagcg ttccatgacc accgaacacc ccgcgccagc
       61 acaccgaata tttcgctgca acagcgccat cgcctcctct tgtcgtcccg catcaccaag
      121 cgcgcgagct gcattgcgaa gcagcacgtc cagatcctcg tgaccgtcgt cgggcacttt
      181 ctgctcgagg atacccacgg cgcgtaacag caactcggca gcaagttcag catctccttg
      241 cctgcgaacg acttccccca gattgttgag tggctccacg agagcgagat gatgagcatc
      301 gacataccgc agcatgatgt cgagagctcg ttcgaacaac cctcgcgccc cagcgagatc
      361 atcgagcatc gtcaaacgaa aagccatgtt ggtgagcacc gaaacgagga tcggatgatc
      421 gttaccaaag ttccgtacgt acagtttcag cgctcgtaca tacagcggtt ccgaatcctc
      481 gaaacgctgt tgctggtcca ggcagtagcc cagattgcac aacaatcccg ccgcatccgc
      541 ggattccgca agccctaggg gctccattcg ttcgagggct tctcgataat ggtcctgcgc
      601 accttgccaa tcaccaagcc cgcaccttgc atcccccacg gctcgaagaa tcggcacaag
      661 atactcctct ccaagctctt cttcgatgac gacggccgcc gcacgattga gaagcatcaa
      721 ggcttgatga taatcacgac gagcaaatgc tcgccgcgcg tcttgaatcc attcttgcag
      781 cggcgcggtc atacgaagaa gctgggacac aatcctttcg gtgtaaagag cacggcgcgt
      841 tgccaatgag atcgttacga aagggaagtg atggaactca agtcgacgcc gcctgttgtg
      901 ctcgttggcg tcgtttctcc atcatgggtg gaatcgtgag gaaaacgatg gctccgatga
      961 ccagcgccgc gccaatgagt tcactggctc ccggcgcacg ttgtccaggg aaaatcagca
     1021 gcaaatacga agctaccaca ccagcgagga tactcgagga tcgattcaca ggaacgcaat
     1081 acgtgttttc tcttcgatcg aggtagatga gattgccgaa caccccggtg ccttgagaaa
     1141 gaaaaccaac gatgaccatg gcgatggcaa tggactgtcc ccaataagcc gtgaatcctt
     1201 tgctcaattc ctcggcgaga ttgcccgaac ctaaaagcgc gtaaatcgca aggatgaccg
     1261 caagggcggg ggtcgccacc atctgttctt ccacgaaata gcgacgattt gtatccgcat
     1321 cgtcggactt ggcaagacgg ctcatgaact gaaggcgaac gaagtagctg gcaagataga
     1381 cgatgatgtc gatcacgcaa ataaagggca tggcgtaaga cttggtatcc acgaggctca
     1441 cgagaagagc gaggatggac atgccgagac ccgcccaggc gaaccagcgc actttgcgtc
     1501 cactgatcgc atcgacaatg ggcgcgatga cgagcacacc accgcgcatg agcaacatgg
     1561 caaaaacgat gctgacccct tcgaacgtat acgcaagggt cgtcgttccc accgtgcagg
     1621 tggcacaaag accggataga agcgtccagc gggaaggcgt gggtatgttg acgccaagga
     1681 ctcgaacgtg attggcgtac ttccaccatc gcatggcgct gacgaaaatg accattccga
     1741 cggctgcagc ggcggccgag atcggcagca ccgcgaaacc ggagattgcc tgaccttcac
     1801 tacccggaac caatcctttg gtgagcattt tggtgatcag ggaatagggc acgtaacacg
     1861 cgaaataacc gaacgcgaaa gcccagatgc tatacgattc catccagctt ttcagggaca
     1921 tgaaacgcct ccatcgcaac gttcgtcagc gatacatcga accaaaggac cttcgaacgt
     1981 tgacgaaaca ccttccttct ccatcggtgt cgaaccgaat gacaagcacg ttccgtggaa
     2041 ttgttggacg aacggacctt gttggatccc accgaccggt ctttgcttcc gacaatcaaa
     2101 tcccaagtgt ttttttagtc ggcgaaagaa tgggttggag atcaaggtat tgtccacaat
     2161 cacaacgaag cgctcagttt ggcaccaggg ttacacgatg acaacaatcg tgtcgaaaaa
     2221 ggactcctcc gatcttcgat tcccgcgatg agcactgcct tttccatcga aaacgcggtt
     2281 tcgtcatcag tcgatcggtc agacttccac ccgcttgcct gtatggtttg tcgaccaacg
     2341 acaccatgcc tcgatccatg gatccggaat tccgcgcctc gaggctcgac cgaaaccgca
     2401 cgagtcgctg gtaccacgcc ggccggtcca aaaaagacaa tcgcatcgcg agcgaatcct
     2461 ttattctttg cccatgcatt cgcttcgtct tcgcaccgtt ctcgggctcg gcttgctcgg
     2521 catcgccgct tgtacgccag cttccctccc gccttcttcc actcatccca cggtcgttgc
     2581 cacaccgccg tctgccttgc caccggactt gtcaccggtg aaggatccta attccatcgt
     2641 gctcgtggca cgccttcgta accttcagga ttcgttgggg acgatggaac ggatgtttcg
     2701 gcttcccatt tccctgacgg acgaactgca aaaggttgtt gcggaaagag cgggagatgc
     2761 acttcagatg gatgccggcg tggatgttgc cgtcgccctg tcacctgctt ccacggacac
     2821 ggatcccaag ttttttgggg cggtttccat cccgatggca aacctcgagg gcgcgctatc
     2881 acagctcgaa aagcgcggga atgtcgtgca tctcgcacct ggcgtctatc gagccaagtt
     2941 cgagcgcccc caatcagagg acggcgagtc gtccgactca ccaacctcgg agtccgaaac
     3001 acctaaagaa tatcgctgtg atttagcagc tagccttgga aatgcgccgg ctcgattggt
     3061 ctgctcggag gatgaacaag ctctggattc gctgcgaccc tggcttaccc gaggcctgcc
     3121 cctgcaaccc acggccaagg aagacctcga agtccacctt cgattcgcac cgctgcgtga
     3181 gcgatatgtg cctgtggctc gaacgcatgc ttccgattac gcaacgaccg caaaacagtg
     3241 gatggaaaca atcctgaaca tccgcgatcc aaagctgctc atgcttcccg aacgggtgat
     3301 gcacgagagc atcacttttg tcgaggatat gcaagaagca tcgtttttcg gcgacttcat
     3361 ccccgaaacg aatgaaatca gcactcgcgt cacgatcgcg tttcggtcgc gcaactcctg
     3421 gttcacccaa gtgtacacgg atgccaatgg acgatcggga ccgccaccgg aaattttctg
     3481 gcggttgccc aaagatgctg ataacgccac ctacgcatcc tgggtcgatc ccgctcattt
     3541 tgaaggtttc aacgatacgg ctcggtcgct gatgtcgcac gcgctggagc tgactccgtt
     3601 gagcgcgcaa tccaagtccg ccatcgatac gttcatcgcg tccattcctc ttcataaaac
     3661 ggtggctgta tcggcgagag gagaagcgcg acgaacgaca tccgcgggga acgttaccac
     3721 ggcgcgcgat gccgtccgcg aattcgaaca gttctttcaa tcgataatcg gctggacgat
     3781 cctagggatg gaagcgccct cggctcctta tgcggaccac ttcaaaaaag gggtggccac
     3841 gatggagcaa ctcatgcatg aggcacgaaa agacgctcat cttggtccag aaatgcgaaa
     3901 ggccacgtgg attcctcaaa tccgatggcg aagcccattt cccggctatc cccagggtac
     3961 gactggcatc gagatcaccg cttcctttga cagcaaagac atctggagct tcgcgaatac
     4021 caagcccggc accccccacc ccccaggggc tgcagcccgc ggcagcatca cgttgcatgg
     4081 cgtcgtcata cccaatggcc ccaatcgcac atggatcggg atttccgccg accccaaaac
     4141 actgcaagaa aaatctcgcg tcgtggtgtc cggtccctcc caaaacaccc tcgcgtccct
     4201 ggcagacctc gaaccccttc gccaatcctc cacgattggg ggagggttta tcatctacgg
     4261 ctcgatggtc gataacatga tgaaaacctt ccctcaaaac cccagcgtcg acatgagatt
     4321 ggttcatcaa tcgctcgaaa ccatgccaaa ccaattacgg acccccgttc tccaactgca
     4381 tacggctacc gctggcgaca ccccgaccaa tacgatcgag ctacggttgc aacgcgggac
     4441 cgtggatgac ctcgccaacc tcattggatt cgtactctcc ccccaaggac aacaactgct
     4501 cgaacagcta cgaaagaccg atatcccgct cgacgttcct tagccgtcga ttacaagggg
     4561 ttgttcgtcg gcaccagtgg ctcggagcga aaccagtccc tgccaccgcg aggttcataa
     4621 gaccgcttcg acacggcttt gacccgcggg tccataacga aatgtcctgt cgatgagata
     4681 ttggcctttc tgttcgacaa cctcgaagct gacggcaact acccctttcg aggtgtgata
     4741 gtggaccacg caacgctgga accaagggcg gccaggctct ttggtttccg tgcattcatg
     4801 cagcgacaat aaagatatct ccccgaattc attacggaat ttctctatcc cttgtcggta
     4861 ttcctcaaca cttacgcgct gttgatactc cgtggtggta taggtcgagc gtgctttctc
     4921 gaaatccccc tgagcaagag cagcctggta gggatcgagc aggtcttttt gaatgcgcgg
     4981 cgcgatttcc tgggcgacga agatttgtcg atacatgcca taaatggcta tcggcagcac
     5041 gagcaagaaa aacactccga ggaggagata tctcgcccaa gcaggcatat cggaggaatc
     5101 atcgtgagac atgagcggca gtgtgccaga ggcggggctg ggagcgcaac cacgacggtg
     5161 tcacgtttct tccacgcgcg aaaaaacagc gaaggcataa gggcgtaacg aaacggtgac
     5221 ttcgccctgg gaaacgaatt ggtctcgtac ggaggcaggg ccgcccccgt gctccgcccg
     5281 aacggaggat tccgaggggc aagcccacaa actgctcgcg gaatccatga ggagccgcca
     5341 gcgcccggaa gaaaaaggaa gagaccgcgt cacggaagcc ccactcacat tcatggcgat
     5401 aatgacctca cggccctcgg cattcctatc gccgatgcgc cggtcccgat cgtcttgatc
     5461 gtaaggatga acccaccggc gaatgaacaa tacccgctcc tcttcgaagc cgcgaacgta
     5521 cgttctgcgc ttgtcgagca cgcgaagggc cggaacggtt ttccgaaggt gaatgagctg
     5581 tcggtacaag cgaaagagca cctcgtgttc ctgccgatcg cgcaagccga ggtcgcattt
     5641 gctgcgaaga aacgtttctt cctcttgcgg atcgggcggc tcttgctgcc agcgaaacgt
     5701 ggcgaattct tctttgcgtc cccgacgcac ggcgtcgatg agcgaaggat ctccgtgatg
     5761 gacgaagtat tgaaacggcg ctatctcgcc gtattcttcc cccatgaata gcaatggaat
     5821 atagggagcg agcaggaccg ctccggccgc caatttgagg gcttcaaagg aaaccaatgc
     5881 ggccagacgc tcccctgccc ttcgatttcc caccatatcg tggttttgac aatacacgat
     5941 cagttggccc gcgcgaatat cccttggacc acgaccatgt ggtcgttttc gatacgccga
     6001 ataatcaccc gcgtaagccc atccttcctg gaccgctcgc acgagttggc taaacgaacc
     6061 gaagtcttcg taatagcctt cctgctcccc tgtgagcaac acatgcaagg catgatgaaa
     6121 atccgcggac cattgggcgt ccatatcata gccgccttgg gaggagggta acaccagccg
     6181 ggggtcgttc agatcgcttt cggcaatgca gtacacccga cgaccgaggc ttttgcctct
     6241 tcgatgtagc gcctcggcca attcttcgag gaacggtcga gcggaaaagt ccttgatggc
     6301 atgaaccgca tcgatacgaa gggcatcgat gtggtaatcc gacacccagt gcaacgcgtt
     6361 ttccacaaaa aagcgtcgta cttcccgaca atgggggcca tcgaagttga tggcgcttcc
     6421 ccaggggatt cgcacttgat cggtaaaata aggaccgaaa tcctgtacat aacagccttc
     6481 gggcccgaaa tggttgtaca cgacatcgag gaccacggcc atcgaacgct gatgacacgc
     6541 atccacgaat cgcttcaatc cgttggctcc tccataggaa tgctgtaccg cgtagggata
     6601 tacaccatcg tatccccaat ttctttcgcc cgagaactgc gccacaggca tgagttccac
     6661 cgcggtgata cccaagtcca ccaggctatc caactgcgcg atggcggcgt cgaacgttcc
     6721 ctcgggggta aaacagccga catgcatttc atagagaata taatcttcga gctgtagccc
     6781 gcaccaaccc gtatcggtcc acggaaagga tggatccact acttccgaag gcccatgcac
     6841 gccttcgggt tgacaacgcg aagcgggatc gggccgttcg atggcatcat cgagcaggta
     6901 agaatagcgt gtgccaggcg caactccttc cacgaccgct tggtggtagc cgaaaggccg
     6961 cggctgaaga cctacccggc ggccatcggg ctcatgcaaa tgaacttgta ttcgagccac
     7021 gaacggagcc caaacgcgaa atagacaacg ttggttaccg aggtacactg cgccgagact
     7081 cggatctccc aatggataca tggcttgacg tcctttcaag ccccgacggg gccaaggcca
     7141 accgcgggcc gaacaagata gcaagggata gcgagtcgcg gagagaaaat cgacgtcgtg
     7201 ccgatgggaa tggatataaa aatcactcga tgacaaaaag gtgagcgggt tgatgccgcg
     7261 ggtccaatcg gacgtagttc gtccacccct gccacgtcca tcgctgccca gaaagcaaat
     7321 cgcgaactcc gtagggctca ttatcccctt tgccgagttc ccaaggattg acatgaacga
     7381 gtgactcgcg cacctcgaag ggattgaggt tgacgaccgc gagaatgacg ttgtcccgtt
     7441 tcggagtggc tttgctatat acgagaatat cgttgctgtc ggccgcgtgg aagcgcagat
     7501 tgtcgtattc ctgcagggcg gggtgctggt ggcgcgcttg attcagcttg gtaatgagtg
     7561 gtttgatatg gccggggcgg tcccaatccc atactttgta ctcatatttt tccgaatcga
     7621 ggtattcctc ccgtccagga atcgctctat tttcacagag ttcgaagcca ttgtagatgc
     7681 cgtagcaaga actgagcgtg gcggcgagca cggcccgaat acaaaaggcc ggacgtcctc
//
LOCUS       DAOSOR010000251         7731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9427_length_7731_cov_4.628973, whole genome shotgun sequence.
ACCESSION   DAOSOR010000251 DAOSOR010000000
VERSION     DAOSOR010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7731
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9427_length_7731_cov_4.628973"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(367..1830)
                     /locus_tag="PK710_13840"
     CDS             complement(367..1830)
                     /locus_tag="PK710_13840"
                     /inference="COORDINATES: protein motif:HMM:NF040132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10854.1"
                     /translation="MFGRYAKWLPFLWTWFAWGCASEQGETSALPSGPLHEAGTDSSS
                     CKPATCLGLGAECGKAPDGCGGVVSCGQCSGGLYCGGDGPNRCGANACVAKTCVSAGA
                     QCGLVSNGCDDVLSCGECVAPLVCGGGGKDNQCGCRAKTCVELNAQCGMVDDGCGMMV
                     DCGACPAGQHCGGEKPNVCGSTACVPKTCQEIGASCGIVSDGCSGTLDCGSCVSPETC
                     GGSGVPNHCGCLKKTCDALNAQCGSVSDGCGGTLSCGDCKTGETCQANECQCANGWKP
                     CGSQCIPNSSCCTDTDCTGDQICVGQACSCPPSTKLCGNQCIPNSSCCSDSDCSGLFV
                     CAKPGDPCSCRTSPSRYPIYRSYRSNGDHLFSVSPDEGTPHGYTSEGVRFYVYAQPCQ
                     WGLVPLRRLYHETKVAHTCATEQSEINALVSQGWRLEGSLGCIATSADCSATALYHLI
                     YASSDLHMFTTSVTERDYMVTDGWTLKGITGYVWGVP"
     gene            1948..3282
                     /locus_tag="PK710_13845"
     CDS             1948..3282
                     /locus_tag="PK710_13845"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT10855.1"
                     /translation="MGHFQDLFPSDCTTLPLFNGELLVSRSHALFCAIPATHVSLVHE
                     LLDGSDDVNLPDSLVNSLRGHGFGGPPRTAKPPSPSVQIQLTNACNLACSYCCTNSGV
                     PRQQEIDRSTAFAIIDDVLSVHGPGTRVSLLGGEPLVVPWAIDLAECIVQHGLQLTIF
                     TNGLLLHDEPLAKRLGALGKQGAQVRVSLAGATAESCDRVSHRPRFDAVIRGIHTLSR
                     FGPPAHVDLMLLPDQVEEVAQQLATLRALLPPQTPIALGIAYLSGREKGANLFASRAE
                     LERALDRISFEAGEVIPAARPQPLADRREGCSCALGHHLHVRSDGTLFTCFKMEERVG
                     DLRSMSFSQGLRSLQKHARPVELLAYCKDCPLATLCGGGCRSENLQYTGDGDVPVCGP
                     WRVRVLSELLAEDHVTALEWPATHLLAEAHARGIEGPAALYPVVPSRHLIDT"
     gene            complement(3288..4106)
                     /locus_tag="PK710_13850"
     CDS             complement(3288..4106)
                     /locus_tag="PK710_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10856.1"
                     /translation="MKSKSLPSLLRKLHEDPEQMAAFLLERSGLPGPRANLELAWTFA
                     DGAKEFNKTVGWKEQMMQWASISPAAAPTNHPQEYLPFVAIQALAELHPEENASGRRL
                     IESMLRQAANDPRWRMREGCAFGLQRIAMNDIQELKSILQHWLEQPSLLEHRAALVAL
                     AHPPLLTNKDTVSFAFGIGDAALQACLSDPRDKEAHRVLRKALEFAPSVFVAADPDRG
                     FPWLERWADKGTLQVAKIVAANLRKARLSRKFPDRVQDVAMRLEACDYSEMPCE"
     gene            4293..7199
                     /locus_tag="PK710_13855"
     CDS             4293..7199
                     /locus_tag="PK710_13855"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOT10857.1"
                     /translation="MIIHGFQLVDERYVAEIKSQARLYRHVQTGAQLLSLINDDENKV
                     FGITFRTPPKDSTGVAHILEHSVLCGSRKYPVKEPFVELLKSSLKTFLNAFTYPDKTC
                     YPVASQNTQDFYNLIDVYLDAVFFPNLTPEVLMQEGWHFEAESESSPLTIQGVVFGEM
                     KGVYSSPDSVLGETTQQSLFPDNTYGLDSGGNPRDIPRLTFEAFKAFHENYYHPSNAR
                     VYFWGDDDPDERLRLVDAYLQQFQAKKIDSTIAIQQPFSSPRYIEKKYASSEPSETDR
                     AMVTVNWLLPEVTDPQTTLAFQILDHALLGTPASPLRKALIDSGLGEDLTGAGVETDL
                     RQMYFSTGLKGIDRNRAEDVETLITKELQALVDQGIPRDLLDASLHSTEFHLREANTG
                     SYPRGLIFMLQSLRSWLYDADPLGPLAFEKPLESIKAKVREGGYFEKLIRDHLLENPH
                     RSRVLLVPDPEEGARRDAQEQQFLSERRKGLTDDDVRQVMEQARHLKERQQTPDSEEA
                     LATIPTLSIEDLPRLGKRIAQERLPLGTQEEAVCFHDQNTFGIAYLDIAFDMSVVPQQ
                     DIPLLPLFGRALLETGTAQQDFVTLSRRISANTGGIWPDMFLSTTQQGTTSAWFMLRG
                     KAMLDKGEELTRIMREVLQSARFEDRERIRQLLLEEKAQMEMGIIPSGHGMVDARIRS
                     RYSEAAWLSEQTGGISYLFFVRDLLSRFEEMWPSVLERLQGLRRRLFNQRHIRVNITL
                     DEAGLGQFKPHVEALFHELPNERVETQTWDRSVDLTSEGLTIPAQVHYVGKGGNLRDQ
                     GYTFHGSALVIGRFLRTSWLWDQVRVQGGAYGAFCRLDRLSGVFTFTSYRDPNLERTL
                     RVFDETAEALARVSLPRKELEKNILGAIGDLDAYMLPDAKGHVAFLRSLTGQTQETLD
                     QIRREVFETEEQHFRDFAAALGRLKEQSTLAVLGGEESIRKVAGPLGLHVIPVL"
     gene            7371..>7731
                     /locus_tag="PK710_13860"
     CDS             7371..>7731
                     /locus_tag="PK710_13860"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOT10858.1"
                     /translation="MRTYESVGVAVPEILLPKQELSKTKWAVVACDQYTSEPEYWQKV
                     EQVVADAPSTLRLIYPEVFLSESAADAEKRISQIRANMQAYLEAGNLVGHDGMIYVER
                     LAGGRIRKGLVLCVDLER"
BASE COUNT         1618 a         2186 c         2229 g         1698 t
ORIGIN      
        1 tatcgcgctg gactgccact caacgaattg aaggggcgcg aaacctacgt tgaactggcg
       61 cgctgcggtg agcaacagag gatgactgcg agcgtggcca cgcgatggta ggtacttgtt
      121 tccggctgtt gacgggcgaa ggaaaggatt gggggaaatg aggggtgaat ttgctgggaa
      181 ttgggaattg ggaactagca actagagact gaactaggaa ctaggaacta gcaactagca
      241 accggtttgt tgcgactgga aaacggcttg gccgtatgcc tgcacaccac gtctttgtgt
      301 ttttccttgc ggtcccctgg ggcctttccc gttccacaga ggcctattta gcaccgccga
      361 tgcgacctac ggcacacccc atacgtagcc cgtaatcccc ttgagcgtcc aaccatcggt
      421 aaccatatag tctcgttctg ttacactcgt ggtgaacatg tgtaaatccg aggaggcata
      481 gatgaggtga tacagagcgg tggcggaaca gtctgccgaa gtggcgatgc atcctagcga
      541 gccttcgagt ctccacccct ggctgaccag agcgttgatt tcggactgtt cggtggcgca
      601 ggtgtgagcg accttcgttt cgtggtacag ccggcgaaga ggcaccaggc cccactgaca
      661 gggctgcgca tacacgtaaa acctcacgcc ttccgacgtg tagccgtgcg gggttccttc
      721 gtcggggctg accgaaaaca gatggtcacc gttggaacga taggagcgat agatcggata
      781 ccggcttggg ctggttctgc aagagcaggg gtcaccaggt ttggcacaaa cgaacaatcc
      841 gctgcaatcc gagtccgaac agcaagaaga gttgggaatg cattgattgc cacaaagctt
      901 ggtggacgga gggcacgagc acgcctgtcc gacgcaaatc tgatctcccg tgcagtcggt
      961 atcggtgcag caggaggagt tgggaatgca ttgggagccg catggtttcc acccgttggc
     1021 gcattggcat tcgttggcct ggcaggtttc tcccgtcttg cagtcaccgc acgacaacgt
     1081 acccccacag ccgtcggaaa cacttccgca ttgcgcgttg agagcatcac aggttttttt
     1141 caggcagcca cagtggttgg ggaccccact gccgccgcag gtttccggcg aaacacaaga
     1201 gccacaatcg agcgtgccgc tacatccatc ggatacgatc ccacaactgg cgccaatttc
     1261 ctggcatgtt ttggggacac acgcggtcga tccgcagaca ttgggttttt ctccgccgca
     1321 atgctggccc gcggggcatg cgccgcaatc gaccatcatg ccgcacccat cgtcgaccat
     1381 tccgcattgg gcgttcaatt ccacgcaggt tttggcgcga cacccgcatt gattgtcttt
     1441 tccgccgccg ccgcaaacga gcggagcaac acactcaccg caagacagca catcatcgca
     1501 accgttggat acgagcccgc attgcgcccc cgcggacacg caggttttgg ctacgcacgc
     1561 gttcgcgccg caccgattcg ggccatcgcc accacaatac aggccgccag agcactgtcc
     1621 gcaagacacc acgccaccac agccatccgg tgcctttcca cattccgctc ccaatcccaa
     1681 gcaggttgcc ggtttgcacg acgacgaatc cgtcccagcc tcgtgcaacg gccccgacgg
     1741 caacgcggac gtttctccct gctcggacgc acaaccccat gcaaaccacg tccacaaaaa
     1801 agggagccac ttcgcatatc tcccgaacat ggtaaacatt atgcccaagg ctccgaactc
     1861 cgcaatgccc gcccgtgcaa agcgcaaaag ccaggaagcc catcggttga caaaaccagc
     1921 cgacccgtcg aagacaacca atgagtcatg ggtcactttc aagatctatt tccttccgac
     1981 tgcaccacgt taccgctgtt caatggcgaa ttgttggtgt cgcgttccca tgcgctattt
     2041 tgtgccatac cggcgacgca cgtgagcctg gttcacgagt tgctcgacgg ttcggatgat
     2101 gtcaacctgc cggattcatt agtgaattct ctgcgtgggc acggcttcgg aggtccccct
     2161 cgaacggcca aaccgccttc gccttccgtg cagattcaat tgaccaacgc ctgcaatctc
     2221 gcttgttcct attgctgcac caactcgggc gtgcctcgcc aacaggaaat cgaccgatcg
     2281 actgcctttg ccattatcga tgatgtgctg agcgtgcatg gccccggtac acgcgtatcg
     2341 cttctcggcg gagaaccgct tgtcgtcccg tgggccatcg accttgcgga atgcattgtc
     2401 caacacggct tgcagctcac catttttacg aacggtttgc ttctgcacga cgagccgttg
     2461 gcgaaacgtc tcggagcgct gggaaaacaa ggcgcgcaag tgcgtgtgtc tcttgcggga
     2521 gccaccgcgg aatcctgcga tcgggtttcg caccggccac gattcgatgc cgtgattcgc
     2581 ggtatccata cgctttcgcg ctttggcccc cctgcccatg tcgacctcat gcttttgccc
     2641 gatcaggtcg aggaggtcgc ccaacaactg gcgactcttc gtgcgctgtt gccacctcaa
     2701 acgcccatcg cgcttgggat tgcctactta tctgggcgtg aaaaaggcgc gaatctcttt
     2761 gcttcgcggg ccgagctcga acgcgctctg gaccggatct cgttcgaagc gggagaggtc
     2821 attcccgccg ctcgaccgca accgcttgcc gatcgtcggg aaggctgttc gtgcgcgttg
     2881 gggcatcatc tgcacgttcg ctccgatgga accttgttca catgtttcaa aatggaagag
     2941 cgcgtggggg accttcgcag catgagcttt tcgcaaggtc ttcgaagtct tcagaagcat
     3001 gctcgacctg tagaattgct ggcctattgc aaagattgtc cgttggcgac gctctgcggg
     3061 ggtgggtgtc gttccgaaaa ccttcagtac acgggcgacg gtgatgtgcc ggtatgtggc
     3121 ccctggcgcg ttcgtgtgct cagtgaattg ctcgccgaag atcacgtgac cgcgctcgaa
     3181 tggccggcca cgcacttgct tgcggaagcc catgcgcgag gaatcgaagg ccccgcggcg
     3241 ttgtatcccg tggtgccctc ccgtcatctc atcgatacat aaatgcgtta ttcacaaggc
     3301 atttccgaat aatcgcaggc ttcgaggcgc atcgcgacat cctgcactcg atcggggaat
     3361 ttccggctca atcgcgcttt tcgcaggttc gcggccacga tcttcgccac ttgcagcgtt
     3421 cccttatccg cccatcgctc cagccaggga aagccgcgat ccgggtcggc ggccacgaag
     3481 acggagggag cgaattcgag tgcttttcgc aggactcgat gagcctcctt gtcccgcgga
     3541 tcagaaagac aggcttgcag ggctgcatcc ccgataccga aagcgaagga tacggtgtct
     3601 ttgtttgtga gcagcggcgg atgggcaagc gccaccaacg ccgcacgatg ctcgagaagc
     3661 gacggttgtt ccaaccaatg ttgtaagatc gatttcagct cctggatgtc gttcatcgca
     3721 attcgttgca acccgaacgc gcatccttcg cgcattctcc agcgagggtc gttcgccgcc
     3781 tgtcgaagca tgctttcgat gagccgacga ccggacgcgt tttcttccgg atggagttcc
     3841 gccagagctt gtatggccac gaaagggagg tattcctggg gatgattggt gggggcggcc
     3901 gcgggcgaga tcgaggccca ctgcatcatt tgttctttcc agcccacggt tttgttgaat
     3961 tccttggcgc catccgcgaa ggtccaagcc aattcgagat tggcgcgagg tccgggcaac
     4021 cccgaacgct ccagaagaaa cgcggccatc tgctcagggt cttcgtggag ctttcgcagc
     4081 aacgagggca ggcttttcga tttcaccgat cgggtcgcta tcatcgttga aagtcggagg
     4141 caatgcggag catggcctca gcagggcatc ggcaagacag ctcttacgat ttggaaggga
     4201 gaggaacgag aacgccactt tcccttgtca ttcttctgca ttaggcattg tgttgctttt
     4261 gcattcattc tgatcgcctt gtcgaggaca tcatgatcat ccacggtttt caactcgtcg
     4321 acgaacggta tgtcgccgag atcaagtccc aagcgcgtct gtatcgccac gtgcagaccg
     4381 gtgcgcaatt gctgtctctc atcaacgatg atgagaacaa ggtttttggc atcacgtttc
     4441 gcacgcctcc caaggactcg acgggtgttg ctcacatcct cgaacactcg gtgttgtgtg
     4501 gttcgcgcaa atatccggtc aaagagcctt tcgtggagtt gctcaaaagc tcgctcaaaa
     4561 cctttctcaa tgccttcact taccccgaca agacctgtta tccggtggcg agccagaaca
     4621 cacaggattt ctacaacctc atcgacgtct atctggatgc cgtatttttc cccaacctca
     4681 cgcccgaggt gttgatgcag gaaggatggc atttcgaagc cgaatccgag tcctcgccgc
     4741 tcacgataca aggcgtggtg tttggagaaa tgaaaggcgt gtattcgtcg ccggatagtg
     4801 tcctcggaga gacgacccaa caatcgcttt tcccggacaa tacctatggc ctcgactctg
     4861 gcggtaatcc ccgcgacatt ccgcgtctca cgttcgaagc gttcaaagca ttccacgaga
     4921 actactatca cccctcgaac gcgcgcgtgt atttttgggg cgacgacgat ccggacgagc
     4981 gtcttcgcct ggtcgatgcg tatcttcagc aatttcaggc aaaaaagatc gattcgacga
     5041 ttgctatcca acagccattt tcttccccgc gatacatcga aaagaagtat gcgtcgtcgg
     5101 agccttccga aaccgaccgc gcgatggtca cggtcaattg gctattgccc gaagtcaccg
     5161 atccgcagac caccctggcg tttcaaatac tcgatcacgc gcttttggga acaccggctt
     5221 cgcccctgcg aaaagctctc atcgattcgg gcttggggga agatctcaca ggggcggggg
     5281 tcgaaaccga tctacggcaa atgtactttt ccaccggcct caaaggcatc gatcggaatc
     5341 gagccgaaga cgtcgaaacg ctcatcacca aggaacttca ggcgttggtg gatcaaggga
     5401 ttccccggga cttgctcgac gcttcccttc actcgacgga atttcatctt cgggaagcga
     5461 atacggggag ctaccctcgc ggactcatct tcatgctgca atcgcttcgt tcgtggttgt
     5521 acgacgccga tcccttgggc ccccttgcgt ttgaaaaacc gttggaatcc atcaaagcca
     5581 aggtccgcga gggcgggtat ttcgaaaaac tcattcgcga tcatcttctc gaaaacccgc
     5641 atcgatcccg ggtgttgctg gttcccgacc ctgaagaagg ggctcgtcgc gacgcgcaag
     5701 aacaacaatt tctttccgag cgcaggaaag gtcttactga cgacgacgtc cgccaggtga
     5761 tggagcaagc gcgtcatttg aaagaaagac aacagactcc ggatagcgag gaagcccttg
     5821 ccaccatccc cacgttatcg atcgaggatc taccgcgtct cggcaagcgc atcgcgcaag
     5881 agcgcctgcc gctgggaacg caagaagagg ctgtgtgctt ccatgaccaa aacacctttg
     5941 gaatcgcgta cttagacatt gccttcgata tgtcggtcgt gcctcaacaa gacattccgc
     6001 tcttgccctt gtttggacgc gcgctgctcg agaccgggac ggcgcaacaa gatttcgtga
     6061 cgttgtcgcg tcgcatcagc gccaatacag ggggaatctg gccggatatg ttcttgtcga
     6121 cgacccagca aggcacgacg agcgcgtggt tcatgctgcg tggtaaggcc atgctcgaca
     6181 agggagagga actgacgcgg atcatgcggg aggtgcttca gagcgcgcgc tttgaagatc
     6241 gagagcgcat tcgtcaactc ttgctggagg aaaaggcgca gatggagatg ggcatcattc
     6301 catcgggcca tgggatggtc gatgcgcgga ttcggtcacg atattccgaa gcggcgtggc
     6361 tcagcgagca gacgggcgga atcagttatc tattttttgt tcgcgatctt ttgtcgcgct
     6421 ttgaggaaat gtggccttcc gtgctcgagc gattgcaggg gttgcgtcga cgactgttca
     6481 accagcggca tattcgtgtg aacatcacgc tggacgaggc ggggcttggt cagttcaaac
     6541 cgcatgtcga ggcgttattt cacgagcttc cgaacgagag ggtggaaaca caaacctggg
     6601 atcgcagtgt ggatctgacg tcggaaggac tgaccattcc cgcccaggtt cattatgtgg
     6661 gcaagggtgg aaatctgcgc gatcaggggt atacgttcca cggttcggcg ctggtgatcg
     6721 ggcggttttt gcggacctca tggctttggg atcaagttcg tgtgcaaggc ggggcgtatg
     6781 gcgcgttctg tcgtctggac cggctgtcgg gagtatttac gttcacgtcg tatcgggatc
     6841 cgaatcttga acggacgttg cgggtgttcg atgagacggc ggaggcgctg gcgcgagtgt
     6901 ctttgccgcg caaggagttg gagaaaaaca ttctgggagc gattggggat ctggacgcgt
     6961 atatgttgcc cgatgccaag gggcatgtgg catttttgcg cagtttgacg ggtcagacgc
     7021 aggaaacgct cgatcagatc cgaagggaag tcttcgagac cgaagagcag cacttccggg
     7081 attttgccgc ggcgttgggt cgactcaagg agcagagcac gttggctgtt cttggcggag
     7141 aagagtcgat tcgaaaggtt gccggacccc ttgggcttca cgtaatcccg gttctctagc
     7201 ttcgaccgtc cacgatttcg cgccacttct ggtatcaccg ggacaaccta ttcatcacgg
     7261 gactgtgcta gggtgcgtcg catctcgatg agcggtgctc gaaaacatcg tccgggcaac
     7321 gacacctcat ctattttcgt tcgccggttt cgaaccggtt aagaggcatc atgaggacgt
     7381 acgaatctgt cggcgtggct gtgccggaaa ttcttcttcc gaagcaagag ttgtcgaaaa
     7441 cgaaatgggc cgtggtcgcg tgtgatcagt acacttcgga gcccgagtat tggcagaagg
     7501 tcgagcaggt ggtggccgat gcaccatcca cgctgcggtt gatctacccg gaagtatttt
     7561 tatcggagtc tgccgcggat gcggaaaagc gaatttctca gatccgcgcc aacatgcaag
     7621 cctatctcga agcgggaaat ctcgtgggtc acgacggcat gatttacgtg gagcggttgg
     7681 cgggcggccg catccgaaag ggattggtat tgtgtgtcga tctcgagcgt t
//
LOCUS       DAOSOR010000252         7701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9475_length_7701_cov_5.112216, whole genome shotgun sequence.
ACCESSION   DAOSOR010000252 DAOSOR010000000
VERSION     DAOSOR010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7701
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9475_length_7701_cov_5.112216"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PK710_13865"
     CDS             <1..463
                     /locus_tag="PK710_13865"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOT10859.1"
                     /translation="SHAIMRQESAFNPEAQSPVGAQGLMQLMPTTADRTAQRMGKPFD
                     LRRLRSPGMNITLGMEYLSMLLRIFDGHVPLAVGAYNAGPMAVGRWLQGAEQTPLDIW
                     VALVPYRETRHYIWRVMSNWLRYRYLLEGEASIPVLGLHLPGSIRIPDDAY"
     gene            complement(469..4404)
                     /locus_tag="PK710_13870"
     CDS             complement(469..4404)
                     /locus_tag="PK710_13870"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT10860.1"
                     /translation="MEETFGRYKLLEPLGQGGMAEVFKAKSFGVEGFEKILVIKRILP
                     QLASNQVFVDMFVHEAKLAVRLSHANIVQVFDLGRVVNDRDVPSYYIAMEYVAGLDIA
                     SLLKRLRRRSEILPVDLCLFVAAEVAKGLDHAHRRRDEQMRPLGIVHRDVSPQNVLLS
                     WEGEVKVTDFGIAKARDLMEDRSDDNKLHQLKGKYAYMSPEQARNQDVDARSDIFSLG
                     AMLYEMIAGVNPFSAATPFETLRRVQAAEFPPIELLREDVSKDLAQIVHRALAKNPED
                     RFENSGRFYEELLGYLYSSGTRYTANDLAEYLSDFRELDAEELKARDVLQDLGPLSDE
                     PDATPVEVPSTAGDHGSAYRSVSSPGFTSTPTAGERREVTVLVLTFAERDWTFPHHLR
                     ARVKDTLARYGARLIDEEPLQLVSLFGLGDTDGRETETAVRCALVLLRQLSAGKVSPS
                     AGVHVGRIMLHASGEPARDTRLMSLVSEAQALARAPEQGCAVSAAVTREIRGLFFLEP
                     LTDVSRAGLTSGAMLVGDLRAPDEVIGKFVGRKNQLRRVGEILAAATRRRLRIVSIQG
                     EQGIGKTRFLYEVHRRLRKGDYNVGFYLASCPPRGRENSLSGLTAMLEVLCGVREGDP
                     LERVLGVEPRLRALGLPDEERNAVLGQLGVKRIGQTGPNLPPLRAAFARMVQRLCEDR
                     LHAFVLDNAHALDEDTLNVLQGSTLRLAATRAVFIMAARTPVQHKLFQHTSHEKIVLD
                     ELDDSEMARLVAVRSGLKTPSPDLIEFFRSRAGGHPLFIEELVKELIETGALVVQNGM
                     AESVKLTGDVAVPRPLRTLMAARVARLEPNHKLALFAAAILGDPIDTTVLATMLGLSL
                     QHNEHTIAALEERALLRRTASSSASLASPMLRESVLESLPVETRRDLHKSAADAYESV
                     FGTRIEQKAHRVAYHLAEYGDRNRAADFFAMSGRRRLRSGQLEAAVHDWVEAFDLSDL
                     EQRNAQELCEWLGDLRAAVHAVRAAPALPRILTRVLSRIDAEANPKRCVIARIHSAAM
                     LGAVHQFDSAQEYIDHALVLADQRVELRRSALLTGAEIAFRRGLFRHALDLYREVSSL
                     GELEGKEAYRLEIGFANTMAAQGNRAEGLTHLDRAQQLSDPTDLADAVEREKIRGLIH
                     YFTRDFRSAAAAFERGADLARASGLSYEVALALHNAGDALVWLGNFARSHVVFCESLS
                     TCEEYGYERLAAHNRMYLAFLEADKGNNDTTVLRELIAIAEAHGYLWDVLDGRHLLGT
                     LAFRRGDTDTARTELGIVQALARDNHNQLLEHSASEMLEQLDREANSTR"
     gene            complement(4479..5414)
                     /gene="miaA"
                     /locus_tag="PK710_13875"
     CDS             complement(4479..5414)
                     /gene="miaA"
                     /locus_tag="PK710_13875"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOT10861.1"
                     /translation="MSASFLLETDDNVLLCVVGPTASQKTALAIRLCEQWDGEVIGAD
                     SVQIYQHFDIGSGKPTARELAQAAHHLVGIVDPREPVDAARYAQLAEASIRDVRARGK
                     VPIVCGGTFLWVRSLVYGLAPAPPASPIVRKKLDKEREQWGDAAMHARLQQVDPITAR
                     RLNPRDWVRVQRALEVFDLSGRPLSEFHQEHQRQAPRYQPRFVGIRWTPAELETRIRH
                     RVRSWLASGWIEEVESLIARGYRHTRPMASVGYRQIVDVLEGRESHEQLLDQIVRATK
                     VFARRQRTWLREVDVQWLDPDSSERPTLELREKDD"
     gene            complement(5411..7267)
                     /gene="mutL"
                     /locus_tag="PK710_13880"
     CDS             complement(5411..7267)
                     /gene="mutL"
                     /locus_tag="PK710_13880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOT10862.1"
                     /translation="MGAILRLSDDLCNQIAAGEVIERPASVVKELVENAIDAAASRIQ
                     IDIGAGGVGLVRVTDDGVGMDEQDARLAVERHATSKIQAVDDLLSLTTYGFRGEALPS
                     IASVSRFTLRTRRRDVDGGIEVTVQAGQVRMTPCGMAPGTIVEVQDLLYNVPARRKFL
                     KSTNAESAAITGVVESLALSQPTVSFALSRDGRACRQWLRASSRHQRAIASRPDDQLE
                     AVTGHRGPLTVEAYLSPPQRARTGAAGLAILINDRVVRDRTLARVVAQAYGSVLEPGR
                     YPVGAVYVDIDPALVDVNVHPQKAEVRFADARAVQDALFRIVTDGLAQAFGLAPASRA
                     FIITPPTAKPSLSSTTPPWLRPSSSSDSPSGQSTVSGDGVGGAHDADPWGLRPERPVP
                     ATAAQPSLGLQPGPWVTPSVAVLPDQSMVRNQEISYGQLRFLAQLRLMFLLCEADDAV
                     VVIDQHAAAERVTFAKLRDAYHKRSVSSQRLLVPATLTLDAEDVAFVETQAEVIESTG
                     LEIRVVGPSQGMVTAVPQILTRADPARLARDLLDEVRRAGGRGFSAAVDLALATMACH
                     GSIRAGDPVSPQEAQSLLSALDDVDFGGHCPHGRPLLMRIRFEELERLVGRR"
     gene            complement(7270..>7701)
                     /locus_tag="PK710_13885"
     CDS             complement(7270..>7701)
                     /locus_tag="PK710_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10863.1"
                     /translation="RISKEASTKALEEHQQRMVPLAVANILLSALLIVACARALAAKR
                     NAHHLALQAVAANILYAIVDYFVSAPVRHAVLTATTSQTLPADAHELDPTQLASAVAW
                     SVRILSAMHVAILGLVAFALTRPRTLAFFHGVEEISPPEEP"
BASE COUNT         1691 a         2158 c         2278 g         1574 t
ORIGIN      
        1 atcccatgcg atcatgcgac aggaaagcgc gtttaacccg gaggcgcagt ctccggtcgg
       61 cgcccaaggg ctgatgcaac tcatgcctac gaccgcggac aggacggcac agcgtatggg
      121 caaaccattc gaccttcgtc gacttcgttc cccaggcatg aatatcaccc ttggcatgga
      181 gtatttatcc atgcttcttc gaatatttga cggtcacgta cctcttgccg taggtgcgta
      241 taacgcgggt cctatggcgg tgggacggtg gctccaaggc gcggagcaaa caccgctgga
      301 tatttgggta gcgttagtcc cctaccgaga gactcgtcac tatatctggc gggtgatgag
      361 caactggtta cggtatcgct acttgctcga aggcgaggca tcgatacccg tgttggggtt
      421 gcatcttcct ggttcgatac gcattcccga cgatgcgtat tgagggaatc atcgggtgct
      481 gttggcttct cgatcgagtt gttcgagcat ttcggaggcg ctgtgttcga gcaattggtt
      541 atggttgtca cgggccaaag cctgcacgat tcccagttcg gtgcgtgccg tatcggtatc
      601 ccctcttcga aatgcgagtg ttcccaacag gtgccgacca tcgagaacat cccacaagta
      661 gccgtgagct tcggcaatcg caatgagttc acgaagaacg gtagtgtcgt tgttgccctt
      721 atcggcctct agaaatgcga ggtacatgcg gttatgagcg gcaagccgtt cgtagccgta
      781 ttcttcacaa gtggataagg attcgcagaa aacgacgtga gagcgtgcga agttgccgag
      841 ccagacgagc gcatcgccgg cgttatgaag cgcgagggcg acttcgtagg aaagtccgga
      901 agcgcgggcg aggtcggcac ctctttcgaa agcagcggca gcggatcgga agtctctggt
      961 gaagtagtgg atgaggccgc gaattttttc tctttcgacc gcatccgcga ggtccgtggg
     1021 gtccgagagt tgttgagcgc ggtcgagatg cgtgaggcct tcggcacgat tgccttgcgc
     1081 ggccatggta ttggcaaagc cgatctccaa gcgatatgct tctttgcctt cgagttcacc
     1141 gagggaggag acttctcgat acaagtcgag ggcgtgtcgg aacagaccgc gacggaaagc
     1201 gatttccgct cccgtgagca aggcggagcg acgcaactcc acgcgctggt cggcaagcac
     1261 gagggcatgg tcgatatact cttgtgcgga atcgaattga tggacggcgc caagcatagc
     1321 ggcgctatgg atgcgtgcga tcacgcagcg tttgggattt gcttccgcat cgattcggga
     1381 aaggacgcgt gtaaggattc gtggcagcgc gggtgccgca cgaaccgcat ggacggccgc
     1441 gcgcaagtcg ccgagccact cacatagttc ttgggcgttt ctttgttcga ggtcactgag
     1501 gtcaaaggcc tccacccaat cgtgaaccgc ggcttcgagt tgcccggagc gcaggcgtcg
     1561 gcgacccgac atggcaaaaa agtccgcggc gcgatttcga tctccgtatt cagccaagtg
     1621 ataagccaca cgatgcgctt tttgttcaat tctggttcca aaaacggatt cgtacgcatc
     1681 ggccgcggac ttatgtaagt ctcgtcgggt ttccacgggc aatgattcga gtacggactc
     1741 tcgaagcatg ggtgaggcga gcgaggcgga cgaggaggcg gtgcgacgca gcagcgctcg
     1801 ttcttcgagg gcggcgatgg tgtgttcgtt atgctgcagg ctcaggccca gcatggtggc
     1861 aagcaccgtg gtatcgatgg gatctccaag gatagcggcg gcaaatagcg ccaatttatg
     1921 attgggttcg aggcgagcca cccgcgcggc catcagcgta cgaagaggcc gaggaaccgc
     1981 gacgtcgccg gtgagtttga cactttccgc catgccgttc tgcacgacga gcgcgcctgt
     2041 ttcgatgagt tccttgacga gttcttcgat gaacagcggg tgtcctcccg cgcgagatcg
     2101 gaaaaattcg atgagatcgg gggatggtgt tttcagtccc gagcggactg ccacgagccg
     2161 tgccatctcg gaatcatcga gttcatcgag cacgatcttc tcatgggacg tatgttggaa
     2221 gagtttatgc tgcaccggcg tgcgcgcggc catgatgaaa acggcacgcg tcgcggcaag
     2281 acgcagggtc gatccttgca gcacattgag ggtgtcttca tcgagcgcat gggcgttatc
     2341 caaaacgaaa gcgtgcagac ggtcttcgca caagcgctga accatacggg caaaagccgc
     2401 acgcaagggg gggagattgg gacctgtttg gccgatccgc ttcactccca gttggccaag
     2461 aaccgcattg cgttcttcgt cggggaggcc gagagcacga agacgaggct cgacccccag
     2521 cactcgttcg agcggatcac cttcccgaac gccgcataaa acttcgagca tcgccgtcaa
     2581 acccgacagg gaattttctc ttccccgggg tggacacgat gccaagtaaa acccaacgtt
     2641 gtaatcgcct tttcgaagac ggcgatgcac ttcatataga aatcgggttt taccgattcc
     2701 ttgttcacct tgtatggaga cgattcgcaa tcgtcggcgt gttgcagcgg caaggatttc
     2761 tcccacacgg cggagttggt tttttcgtcc gacgaacttt ccaatgactt catcgggggc
     2821 tcgaaggtca ccaacgagca tggcgccgga tgtcaatccc gcgcgagaaa catcggtgag
     2881 cggttcgaga aagaaaagtc cgcggatttc gcgtgtgaca gccgcggaca cggcacagcc
     2941 ttgttccggt gcgcgggcaa gcgcctgggc ttcgctgacc agcgacatca atctcgtgtc
     3001 tctcgccggc tctcccgagg catgcagcat gatgcgaccg acatgaacgc ccgcgctcgg
     3061 agatactttc ccggcgctga gttggcgcaa aagtacgagt gcacaacgaa ccgccgtttc
     3121 ggtttctcgt ccatccgtat cgccgagtcc aaaaaggcta accaactgca agggctcttc
     3181 gtcgatgaga cgggctccgt agcgcgcaag ggtatctttc acacgagcac gcagatggtg
     3241 tggaaacgtc caatcccttt cggcaaacgt caggaccaac accgtaactt cacgtcgttc
     3301 cccggcggtg ggcgtgctgg tgaagcctgg ggaactcacg gatcgatacg cggaaccatg
     3361 gtcaccggcc gtggagggca cctccaccgg tgttgcatcc ggttcatcgg acaaaggtcc
     3421 caggtcttgg agcacatcgc gcgctttgag ttcttccgca tccaattcgc ggaagtccga
     3481 aagatactct gccaagtcat tggccgtata acgcgtgccg gaggaataca aatacccaag
     3541 aagctcttcg tagaatcgac cggaattttc gaaacgatcc tcgggatttt tagcaagcgc
     3601 tcgatgcacg atctgggcaa gatccttgga tacatcctca cgaagcaatt cgatgggagg
     3661 aaattccgcg gcctgcaccc ggcgaagtgt ctcgaagggc gttgccgcac tgaacggatt
     3721 gacccccgcg atcatttcat aaagcatggc accaagcgaa aaaatatcgc tgcgcgcatc
     3781 aacgtcctga ttgcgcgctt gctcaggact catgtacgca tacttaccct tgagctgatg
     3841 aagcttattg tcgtcggatc ggtcctccat gaggtcacga gccttggcaa ttccaaagtc
     3901 cgtaaccttc acctccccct cccacgacaa caatacgttc tgcggtgaaa cgtcgcgatg
     3961 aacgatgccc aaaggacgca tttgctcgtc tctacggcga tgggcatgat cgaggccttt
     4021 ggcgacttcc gccgcgacga aaaggcatag atctacgggc aagatttcgg aacgacgacg
     4081 caaacgtttg agcaaagagg caatgtcgag ccccgccacg tactccatgg caatgtaata
     4141 cgatgggaca tcacgatcgt tgacgacgcg acccaagtcg aagacttgga cgatattggc
     4201 atgcgagagg cgtacggcga gtttggcctc atgcacgaac atatcgacaa agacctggtt
     4261 ggaagcgagt tgtggcaaga tcctcttgat gacgaggatt ttttcaaatc cttccacgcc
     4321 aaagcttttt gctttgaaca cttcggccat tccaccttgg ccgagcggtt caagtagctt
     4381 gtatcgcccg aacgtctctt ccacggccag agtacctcaa agtcccctta tgagtatctt
     4441 tcgtagctta tcacccttct ttggattggg cgaatcgttc aatcgtcctt ttcgcgcaat
     4501 tccaatgtgg gtctttcgct actgtctgga tccaaccatt gcacgtccac ctcccgcaac
     4561 catgttcgtt gccgtcgggc aaataccttg gtggcacgca cgatttgatc gagcagttgc
     4621 tcatgactct cgcgcccttc caggacatcg acgatttgcc gataacccac cgatgccatg
     4681 ggacgcgtat gacgatagcc acgcgcgata agtgattcga cttcctcgat ccatcctgac
     4741 gcaagccagg accgtacacg atgacgaatc cgggtttcca actccgcggg tgtccatcga
     4801 atccccacaa agcggggttg ataccgcggc gcttgacgtt gatgctcctg atggaactcg
     4861 gataaagggc gtccgctcag atcgaagact tccaacgcac gttgaacgcg aacccaatcc
     4921 cttggattca gccgccgcgc cgtgatcggg tcgacctgtt gcaaacgagc atgcatcgcc
     4981 gcatcccccc attgctcacg ctctttatcg agtttttttc ggacgatggg cgaagccggt
     5041 ggcgctggcg cgagcccata caccaaggaa cgaacccaaa gaaacgtccc gccgcagacg
     5101 atcggaacct ttcctctcgc gcgcacatcc cggatcgacg cttccgcaag ttgcgcatat
     5161 cgagcagcgt ccaccggttc ccgtgggtcc acaatcccta cgagatgatg ggcagcttgc
     5221 gccaattctc gagctgtcgg cttgccagaa cctatgtcga agtgctgata gatctgcacg
     5281 ctgtccgcac cgatcacctc accgtcccat tgctcgcaaa gacgaatcgc cagagctgtt
     5341 ttttgcgatg ccgtgggccc cacgacgcaa agcaaaacgt tgtcatcggt ttccaaaaga
     5401 aacgatgcac tcatcgtcgt cccaccagac gttcgagttc ttcgaaacga atgcgcatga
     5461 gcaaaggacg accgtgagga caatgcccac caaagtccac gtcatccaac gcggacaaca
     5521 aggattgggc ctcttgcgga gatacgggat caccggcgcg aatcgatcca tggcaggcca
     5581 tggtggccaa cgccagatcg actgcggcgg aaaagccacg cccacccgcg cgtcgaacct
     5641 catccaacaa atcacgagca agccttgccg gatcggccct cgtcaagatc tgagggaccg
     5701 ccgtgaccat cccttgcgaa ggccccacca cacgaatttc caatccggtg gactcaatca
     5761 cctccgcttg ggtttccacg aacgccacat cctccgcgtc caacgtcagc gtggcgggaa
     5821 cgagtaagcg ctgagaactc accgatcgct tgtggtaggc atcacgaagc ttggcaaacg
     5881 tgactcgttc cgccgcggca tgctgatcga tcaccacgac ggcatcgtca gcctcacaca
     5941 gcaaaaacat gagccgaagt tgcgccagaa aacgaagttg gccgtaacta atctcttggt
     6001 tacgaaccat cgattgatct ggcaatacgg ctaccgacgg cgttacccac ggtccaggct
     6061 gcaagccgag cgatggttga gcagcagtgg cgggcacggg tcgctcaggt cgtaaccccc
     6121 aaggatcggc gtcgtgggcc ccccctaccc catcgcctga aaccgtggat tgtccggatg
     6181 gtgagtccga agaggaagac ggacgcaacc acggtggcgt ggtcgaagac aacgagggct
     6241 tcgcagtcgg aggggtaata atgaatgcgc gagaagcggg tgcaagaccg aatgcctgag
     6301 caagaccatc ggtaacgatg cgaaacagag catcctgtac ggcgcgggca tcggcaaaac
     6361 gcacttctgc tttttgggga tgcacgttga catcgaccag ggcagggtcg atatccacgt
     6421 acacggcgcc gacaggataa cgtccgggtt cgagcacgct tccgtaggct tgcgctacca
     6481 cacgagccag ggtccgatcg cgaaccacac ggtcattgat gagaatcgcg aggccagcgg
     6541 caccggttcg agcacgttgt ggagggctaa gataggcttc tacggtcaga ggtcctcggt
     6601 gacccgtcac ggcttcgagt tggtcatcgg ggcggcttgc gatggctctt tggtgacgcg
     6661 aagatgctcg cagccattgt cggcaagcgc gcccatcgcg gctgagcgcg aaggagacgg
     6721 tgggttgtga gagcgcaagg ctttctacga ctccggtgat ggcggcggat tcggcgttgg
     6781 tggatttgag gaactttctt cttgccggca cgttgtagag caggtcttgc acttcgacga
     6841 tcgttcctgg cgccataccg caaggggtca tgcgcacctg accagcctgc acagtcactt
     6901 cgattccgcc atcgacatcg cgacggcgcg tgcgcaatgt gaatcgggac acggaggcga
     6961 tgctcggcaa ggcttcgcct cggaagccat aggtggtcag ggacaataag tcatcgacag
     7021 cttgaatttt gctggtggcg tgtcgctcta cagcgagtcg ggcatcctgt tcatccatgc
     7081 cgacaccatc atcggtcacg cgcaccaaac cgacacctcc cgcgccaata tcgatctgga
     7141 tgcgcgaagc agcggcgtcg atggcgtttt cgacgagttc tttgacgacg ctggcgggtc
     7201 gttcgatgac ttctccagcg gcgatttgat tacatagatc gtcggataaa cgaaggatcg
     7261 cgcccacgtt cagggctcct ccggaggcga tatttcttcg acaccgtgga agaaagcgag
     7321 ggttcgcgga cgggtgaggg cgaaagccac gaggccgagg atggcgacat gcatggcgga
     7381 caagatacga acggaccatg caacagcgga agcgagttgc gtaggatcca attcgtgagc
     7441 gtcggcaggc aaggtttgcg acgtggtcgc ggtgagcacg gcatggcgaa caggcgcgga
     7501 gacgaagtaa tccacgatgg cgtacaggat attggcggcg acggcttgga gcgcgaggtg
     7561 atgagcattg cgtttggcgg caagggcgcg ggcacaggcg acaatgagca aagcggagag
     7621 caagatattg gccacggcaa ggggcaccat gcgctgttga tgttcttcga gagctttggt
     7681 ggaagcttct ttggaaatgc g
//
LOCUS       DAOSOR010000253         7701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9476_length_7701_cov_4.333508, whole genome shotgun sequence.
ACCESSION   DAOSOR010000253 DAOSOR010000000
VERSION     DAOSOR010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7701
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9476_length_7701_cov_4.333508"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1808)
                     /locus_tag="PK710_13890"
     CDS             complement(<1..1808)
                     /locus_tag="PK710_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10864.1"
                     /translation="MIASSAVAFIVLLVLGFFPLVGGPGYESSLVCGLLLPSLAAVAA
                     AFFSKVPDTQPSEVFVRSIENALLLSTVALLTTLLHGLRVGFCDASTGITLFLLGPAV
                     GAVMGACWGFVVGQVMPSLGSRRLRNILLIMLSLLGPALGVGISLIRFYTSPMVFAYD
                     PFFGFFSGTLYDTNVTDNLVTLLTYRAGSACSIIAVGSAAFLFHRSESGRLHFSKQRH
                     PGVLWLSVATTMTSVILNIEGSRLGHWQTANTISNALGASLFDDRCELIYPRSVDHEL
                     AKLLLRDCSTQLYEVKTALEIDSAPRIRVYMFHSPEQKRMLTGAGRTSVAKPWRKEVY
                     VHLDEYPHPILGHELAHVLAGTWARGPFSVAGRLGGWWPDPGLIEGIAVAASPDDDVL
                     TPHHWSSAMLKLDVLPSLDTVFALGFLNKNSSMAYTVAGAFVQWIADRHGMQAIRRWY
                     AGERLEEVAHDSLQNLEEAWRNDLRQVTLSEAELAYAKARFDRPGVFQRRCPHTVDAL
                     NDEGQVLASQGDCSTAMLRFGQALKLDPVHPRTRMNQASCAQRSLGFAEAKGQWKAIE
                     DDTMLPDTTRYRANEALADLYFMQGQAAKADEIYTS"
     gene            complement(1884..2066)
                     /locus_tag="PK710_13895"
     CDS             complement(1884..2066)
                     /locus_tag="PK710_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10865.1"
                     /translation="MPLPASTNTSSVLSSVPHESVPQAVALPTISHVILPLTRILDRT
                     ASLVLFMENAIKIACH"
     gene            2088..3989
                     /locus_tag="PK710_13900"
     CDS             2088..3989
                     /locus_tag="PK710_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10866.1"
                     /translation="MVHCVGCGALNTLEGARFCVQCGARLPVQMEPSEVAPSPAPIDV
                     RFPAPVPGPPPAHPQQPTPESLRMMTGARELAAPSSYSFVGNRSVERPSIHDESYAAQ
                     TSGTHAQRLADAVAQASLTQSHSEPRGPTDLGPSERSIESSLHGPEVSKPSHEPLVFA
                     QTISRAASTPVPELATRTLPAIEHVADGVSKPSTEPRVVVKSVPDKAALADTVSVSSL
                     SSSPVDAAEPQASSVPKGEQSRIGSPQRPMYSPIVKKAAASAFGFDAPEDDLSWAPPP
                     SGQEPAVSPLESSPSRSFDSPVVVASPSKSAEATFKQEVNEADIAGLLDDIDAGFDRI
                     VSSPGVDAKVEMTEGESQEVIELFTNIAAGYMRPVRDFMVELGLGEPPREWIDVVKPA
                     VASLGKSGKVMGLSELGAATEVFLEALTSVENSEGTRFTAEHVKELTEAFELLAAELP
                     GAFNLEQERERREPIIVQSLLRQIPDVRKVALDKLYSANLTTLSMYYHAKPYDIAQAA
                     GLSEELAARIVSHFSRYREQTSVVTPDLERSQEHEKLTALMTLLQQQNDDFEAASKSW
                     SAHAMQDKKRLRKERNDTVLEINLLLARLGEVDLVKQIEKLPLHAKVRALQAYLDEHK
                     EHVAADSRR"
     gene            4017..5255
                     /locus_tag="PK710_13905"
     CDS             4017..5255
                     /locus_tag="PK710_13905"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOT10867.1"
                     /translation="MADYSRDQVLAKVKSGEGLARADLRGLDLSETELLSVDFRRSDL
                     EGANFENAKLDEANLANANLVEAYLKGASLRGAKLDKADLDSANLERANLQGADLKRV
                     SLEGATLTEANLSQALVINASLEMADMGGATLEGASLSHAQMNEAYLGAVKAKAADLS
                     NADLSSANLEEANFESANLTEANLTDTKAPGAVFAKACLLRINATNASLAGADLSFAD
                     LRGADFSNADLHGATLTGAKVFGATFSHANLDDLLLEHLDISSDGDDSRRISGEAAMQ
                     WLQSGRPSAEIPGRRYFGKGDVMRNAALEFGDNSLVEIESRFEKCSITLGDGTELIVG
                     EAGVLRSCTIKGAGNIIIHGRFLEDNSPGIIGPKRLIVSSRGAIHATVKQPEGHTQFA
                     FEPGSKLRLNIVRPDDEKGR"
     gene            5262..5630
                     /locus_tag="PK710_13910"
     CDS             5262..5630
                     /locus_tag="PK710_13910"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOT10868.1"
                     /translation="MADLKSGEKQTLVEEGTQFKGSLTSSCPVVVRGKIEGEIETPSL
                     TISETGAVHGKVTVGAVVSMGDIAGEYDADSVQLSGSVQDNTVIRAKTLEVKLSSEKG
                     KMEVVFGNAKLSVGDEPEDS"
     gene            complement(5669..6046)
                     /locus_tag="PK710_13915"
     CDS             complement(5669..6046)
                     /locus_tag="PK710_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10869.1"
                     /translation="MTAEPSEATPFHATWQVGSCEVEMRQGLGSTYRFAEAQRSRVEQ
                     ELQRSLSECMQKRGGVQGTLYVQAKVAESGALSEVVVSPGGAIPTDLVGCMSQSLDGL
                     RLASPHEGGSSLMVFVVSACALP"
     gene            complement(6270..7160)
                     /locus_tag="PK710_13920"
     CDS             complement(6270..7160)
                     /locus_tag="PK710_13920"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT10870.1"
                     /translation="MLATLPSRSRSMVIVGIVVSCAKMSCAKTDPVETKSSSSVEQVV
                     LSASAEPVEHAESMVLERPKDQCPSSMILVQSSFCIHRWEAHLIDDRGQMHDPYRVPP
                     FPEQMGEWRAHALPGVVPQGYTGRDQAEIACKNAGFRLCRQHEWQIACRGPQGMIFPY
                     GTQHRIERRCNTHKFPGPSHLVLYFYPHTTWSTSEMNDPRINQVPDGLAPTGQYVDCT
                     NAYGTFDMVGNLQEWVFDLVSEKEHKGNAIALGDHYMGQGRNYEGCEARNVAHGYYPN
                     EPTRNQRDYSTGFRCCADPQ"
     gene            7253..7654
                     /locus_tag="PK710_13925"
     CDS             7253..7654
                     /locus_tag="PK710_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10871.1"
                     /translation="MKNVQTRPPSFPQAPLHQPLAWEKQTQWQVLCGDTHHWRMGMYS
                     PKETSRAELHNLERHDCPELFVLLHGSLTLVIAESSGLREIVLEPHVPVLVAAPHAGY
                     CPRGPYSGVALVVERDSFDTEYRPARSWIES"
BASE COUNT         1815 a         2188 c         2123 g         1575 t
ORIGIN      
        1 aaactcgtgt agatctcgtc ggctttggca gcttgtccct gcatgaagta caaatccgcc
       61 aacgcttcgt tcgcacgata tcgggtggtg tctggaagca tcgtgtcgtc ctcgattgct
      121 ttccactgtc ctttggcttc cgcgaacccg agcgaacgtt gggcgcatga tgcttgattc
      181 attctcgtcc gaggatggac gggatcgagc ttcaacgctt gtccgaatcg cagcatggcg
      241 gtgctgcagt ctccttgcga tgcgagcact tgtccttcgt cgttgagggc atcgaccgta
      301 tgtggacagc gacgttgaaa cacacctggt cgatcgaaac gcgctttcgc ataggcaagt
      361 tcggcttcgg aaagcgtgac ttgtcgtaga tcgtttcgcc atgcttcttc gaggttttgc
      421 agactgtcgt gagcaacttc ctccagccgc tcacctgcgt accagcggcg tatggcctgc
      481 atgccgtgcc ggtcggcaat ccattgcacg aaggcaccgg cgaccgtgta ggccatcgat
      541 gagtttttat tcaaaaaacc aagggcaaac acagtatcca aagaggggag aacatcgagt
      601 ttcagcatgg cggaagacca atgatgcggt gtcagcacat cgtcatccgg tgaggcagcc
      661 acggcgatgc cttcgatcaa tccagggtcg ggccaccagc cgccgagtcg gccggctacg
      721 gaaaaaggac cacgagccca tgttcccgcg agcacatggg caagctcatg acccaggatg
      781 gggtgtgggt attcgtccag gtgaacgtag acttctttgc gccagggctt ggcgacagag
      841 gtccgccccg cgccggtcaa catccgcttt tgttccgggc tgtggaacat atagacacga
      901 atgcgtggag cagaatcgat ttcgagcgcg gttttgactt catatagctg ggtcgagcag
      961 tcacggagca acagcttcgc caactcatga tccaccgatc gtggataaat gagttcacaa
     1021 cgatcgtcga acaacgatgc tccaagcgcg ttggatatgg tattcgccgt ttgccaatga
     1081 cccaggcgac tgccttcgat gttgaggatg acgctggtca tggtggtagc aacactcagc
     1141 caaagcacgc ctgggtggcg ctgcttggaa aagtgcaaac gacccgattc gcttcgatgg
     1201 aagagaaagg cggcgctgcc gacggctatg atcgaacagg cgcttcctgc ccggtaggtc
     1261 agcagcgtga caaggttatc cgtcacgttc gtgtcataga gtgttccgga aaaaaatccg
     1321 aaaaacggat cataagcaaa caccatggga ctcgtataaa aacgaattag gctgatcccc
     1381 acgcccaacg ccggacccaa tagcgagagc ataatcagca aaatatttcg caatcttcgg
     1441 gaaccaagcg atggcatcac ctggcccacg acgaatcccc aacaagctcc catcacggcg
     1501 ccgacggccg gaccaagtag aaatagtgta atgccggtgc tggcgtcaca aaacccaacc
     1561 cgaagaccat gaagcaaggt tgtcaacaac gctaccgtgg agagtagcaa cgcgttttcg
     1621 atcgaccgga cgaatacttc gctgggttga gtgtccggta ccttggaaaa aaacgcagcc
     1681 gctacggccg cgagcgacgg gagcagaagc ccgcagacca acgacgattc gtagcctggg
     1741 ccgccgacga ggggaaagaa cccgagcacc aacagcacga tgaacgcgac cgcggaactc
     1801 gcgatcacgg gcggtgatcg ataaagagga agcatgcctt tcaaactagg cctatccctc
     1861 gaaccccaga gaaacaaggg ggatcaatga caagcgattt ttatggcgtt ttccatgaac
     1921 aacacgagac tcgccgtccg atcgagaatc cgtgtcaacg ggaggatgac gtgtgatatc
     1981 gtcggaagtg cgactgcttg cggtaccgat tcgtgaggca ctgacgaaag gacagatgag
     2041 gtgttggtgg atgcgggcag aggcatgagg cctcgatgga ggcgaacgtg gtccattgcg
     2101 taggttgtgg cgcactcaac acgctggaag gagcacggtt ttgtgttcaa tgcggtgccc
     2161 ggctcccggt tcaaatggaa ccgtcggagg ttgctccttc tcccgcgcct atcgatgttc
     2221 gctttcctgc gccggtgcct ggtccaccgc cagcacaccc ccaacagcct acccctgaat
     2281 cactcaggat gatgactggt gcgagagagc ttgccgcgcc ttcttcgtat tccttcgtag
     2341 gaaaccgttc cgtggagcga ccatcgattc acgatgaatc ctacgccgca caaacgtccg
     2401 gcacgcatgc ccaacggctg gcggacgccg tagcgcaagc atccttgacg caatctcatt
     2461 cggaaccccg cggtcctact gacctcggcc cttcggaacg aagcatcgag tcgtccttgc
     2521 acggaccgga agtatcgaaa ccatcgcacg agcctttggt attcgcgcag acgatctccc
     2581 gtgctgcttc cacgccggtg cctgaactgg cgacgcgcac gttgcccgcg attgaacacg
     2641 ttgcggacgg cgtttctaag cccagcacgg agccgcgggt cgtcgtgaaa tccgttccgg
     2701 acaaagccgc cttggccgat acggtgagtg tttcgagcct atcaagctct cccgtcgatg
     2761 cagcggaacc acaggcatct tcggtcccta agggagaaca atcacgtatc ggttcacctc
     2821 agcgcccgat gtattcgccc atcgtgaaaa aagcagcggc gagcgcattc ggcttcgacg
     2881 ctcccgaaga tgacttgtct tgggctccgc cgccctcggg tcaggagccc gcggtatccc
     2941 cgctggaatc atccccatct cggtctttcg acagcccggt tgtcgtggcg tcaccgtcaa
     3001 aaagtgccga agcaacgttc aagcaagagg tgaatgaagc ggatatcgcg ggactgctcg
     3061 acgatatcga tgcgggattc gatcgaatcg tttcgagtcc tggtgtcgat gcgaaggtgg
     3121 aaatgaccga gggggaatcg caagaagtca ttgaattatt tacgaatatc gctgctgggt
     3181 acatgagacc ggttcgtgat ttcatggtag aattggggct tggtgaaccg cctcgtgagt
     3241 ggatcgatgt ggtcaaaccc gccgtggcat ccctagggaa atccggcaaa gtcatgggtt
     3301 tgtcagagct tggagcggct accgaggtgt ttctcgaagc gttgacgagc gtggaaaaca
     3361 gtgaaggaac gcggttcacg gcagagcatg tcaaagagtt gaccgaggca ttcgagctgt
     3421 tggcagcgga acttccgggt gcattcaacc tcgagcaaga gcgcgaacgc agggaaccca
     3481 tcatcgtgca atcgttgcta cggcagatcc cggatgtgcg aaaggtcgct ctcgacaagc
     3541 tgtattcggc aaatcttaca actctgtcga tgtattatca cgccaagccg tatgacattg
     3601 ctcaagcggc agggttgagt gaggagttgg cggctcggat cgtttcgcat ttttcgcggt
     3661 atcgtgagca aacctcggtg gtcacgccgg acctggagcg ctcgcaggaa cacgaaaaac
     3721 tcacggcgct gatgacgcta ttgcagcagc aaaacgatga cttcgaagcg gcatcgaagt
     3781 cgtggtccgc gcatgcgatg caggataaga aaaggttgcg caaagagcga aacgatacgg
     3841 tattggagat caacctcctt ctcgcaaggc tgggggaggt ggatctcgtc aagcagatcg
     3901 agaaactgcc gttgcacgcc aaggtgcgtg cattacaagc ctatttggat gagcataagg
     3961 agcacgtcgc ggccgattcg cggcgttgaa aaacaaggaa gccacaagga ttaacgatgg
     4021 cggactactc ccgagaccaa gtgcttgcga aagtaaaaag cggtgagggg cttgcacggg
     4081 ccgacttgcg gggcctcgat ctgtccgaaa ccgaactact ttctgttgat ttccgacgat
     4141 cggacctgga aggggccaac ttcgagaatg caaaactcga tgaggccaac ctcgcgaacg
     4201 ccaatttggt agaggcgtat ctcaaagggg cgagcctgcg gggggcaaaa ctcgacaaag
     4261 cggatctcga tagcgccaac ctcgaacggg ccaaccttca aggggccgat ctcaaacgcg
     4321 tttcgctcga aggggcaacg ctgacggaag cgaatctgtc gcaggccctc gtcatcaacg
     4381 caagcctcga aatggcggat atgggagggg ctaccctcga aggtgcttcc ctttcgcatg
     4441 cgcagatgaa tgaggcttac ttgggagccg tcaaagccaa ggctgccgac ctcagcaatg
     4501 ccgacctgtc cagcgccaat ctcgaagaag ccaacttcga aagcgccaat ctcacagaag
     4561 ccaacttgac cgacaccaaa gccccaggag ccgtttttgc caaggcttgc ctattgcgta
     4621 tcaatgccac gaacgcatcg ctcgcgggag cagacttgtc cttcgccgat cttcgaggcg
     4681 cggacttctc caacgccgac ttgcatggcg ccaccctcac cggggccaaa gtctttggcg
     4741 ctacgttctc tcacgccaac ctcgatgacc tgctgctcga gcacctcgat atcagttccg
     4801 acggcgacga ttcgcgtcgg ataagcggtg aagcggccat gcaatggctc caaagcggtc
     4861 gcccttccgc cgaaatccct ggccgacgct acttcggaaa aggggatgtc atgcgcaatg
     4921 cggctctcga attcggcgat aactccttgg tcgaaatcga aagcagattc gaaaaatgct
     4981 cgatcacact cggagacgga accgaattga tcgtcggaga agccggcgtg ttacgaagct
     5041 gcaccatcaa aggcgccggc aatatcatca ttcatggccg atttctcgaa gacaatagcc
     5101 cgggtatcat cggacccaaa cgcctcatcg tcagctcccg tggggctatc catgccaccg
     5161 tcaaacaacc cgaaggccat acccaattcg cttttgaacc tggttcgaaa ttacggctca
     5221 acatcgtccg cccggacgac gagaaaggta ggtagcgtcg aatggctgat ctcaagagcg
     5281 gggaaaaaca aaccctcgtc gaggaaggaa cccagttcaa gggctcgctt acatcctcct
     5341 gccccgtcgt cgttcgggga aagatcgaag gagaaatcga gaccccttct ctcaccatca
     5401 gcgaaacagg tgccgtccac ggaaaggtta ccgtaggtgc cgtcgtttcc atgggggata
     5461 tcgccggaga gtatgacgcc gattccgtgc aactatccgg atccgtacag gacaataccg
     5521 tgattcgagc caaaaccctc gaagtcaagc tttcttccga gaaaggaaaa atggaagtcg
     5581 ttttcggaaa tgcaaagctt tccgtcggcg atgagcccga ggactcctga tcgtcaccgc
     5641 ctttgatgca tgccttgccg aacccgctct agggcaaagc acacgcgctt accacgaaca
     5701 ccatgagtga tgatccgcct tcgtgaggcg acgctagccg taaaccatcc aacgattggc
     5761 tcatacaacc aaccaaatcg gtaggtatcg caccaccggg agatacgacc acctccgaaa
     5821 gcgctcccga ttcagcgacc tttgcctgaa catagagcgt tccttgcacg ccgccacgtt
     5881 tttgcatgca ctccgacagt gaccgctgta gttcttgctc cacacgactc cgctgcgctt
     5941 ccgcaaaacg atacgtggac ccaagtcctt gccgcatctc cacttcacac gacccaacct
     6001 gccaagttgc atgaaacggt gtcgcctccg atggttcggc agtcatactc gtttgctttg
     6061 aggaggagcc ttccgcctgt gcaaattcgg aaggaggctt cgccccagca caacccattg
     6121 ccgctgcacc ggccgttgaa atcaatgcga tgaggattcc caagatccaa aaccagcggc
     6181 gctgtcgtcg tcccatcgcg cccaagacta acctgggcag aacgattcga ccagtcatca
     6241 tgccgtggtg tgacgccaat atctttcgat cattgcggat ctgcacaaca acgaaacccc
     6301 gtggaatagt cccgttggtt gcgagtgggt tcgtttggat agtagccgtg agctacgttt
     6361 ctggcttcac agccttcata atttcgtcct tgccccatgt aatggtcgcc cagggcaatg
     6421 gcgtttccct tatgctcctt ctctgacacc aaatcgaaaa cccattcctg taaattgccc
     6481 accatatcga acgttccgta ggcattcgta caatcaacat attggcccgt gggcgcaaga
     6541 ccgtcgggaa cttgattgat gcgcgggtca ttcatctcgg acgtgctcca cgtcgtgtga
     6601 ggatagaaat acaacaccaa gtgggaaggg cctgggaatt tatgcgtgtt gcatcgccgt
     6661 tcgatacggt gctgagtccc gtacggaaat atcatccctt ggggaccgcg acaggcaatc
     6721 tgccattcgt gttgcctgca aagccgaaag cccgcatttt tacaggcgat ctcagcttgg
     6781 tcgcgacccg tatacccttg cggtacaacg ccaggtagtg catgtgcgcg ccattctccc
     6841 atttgttcgg gaaacggcgg aacacgataa ggatcgtgca tctgtccccg atcatcgatc
     6901 aaatgagctt cccaacgatg aatacagaac gatgattgaa ccaggatcat cgaggaagga
     6961 cattggtcct tggggcgttc gagaaccatc gattcagcgt gttcgacagg ctccgcactt
     7021 gccgaaagca caacctgttc cacagaagat gacgacttcg tttcgacggg atccgttttt
     7081 gcacaagaca tttttgcaca agacacgaca atgcccacaa tcaccatcga acgggatcgg
     7141 gatgggagcg tcgcgagcat ctgtcgtgca taatctttcc tccccaaagg tccaagctcg
     7201 ttgcgcaaaa cacgtggctg acaccgtttg tacttcttga taaagcaaag atatgaaaaa
     7261 cgtccaaacg aggcctccct cgtttcccca agctccgctt caccaaccgc tggcctggga
     7321 aaagcaaacc caatggcagg ttctttgcgg cgatacccat cattggcgta tggggatgta
     7381 ttcaccgaaa gaaacgtctc gcgcggaact tcataacctc gaacgccatg attgccccga
     7441 gttgttcgta cttcttcacg gaagcttgac ccttgtcatc gccgaaagca gcggcttgcg
     7501 ggaaattgtg ctcgagcccc atgttcccgt gctcgttgcc gccccccatg ccggctattg
     7561 ccccagggga ccgtattccg gcgtggccct cgtggtcgaa agggattcgt tcgacaccga
     7621 ataccgccct gcgcgttcgt ggatcgaatc atgaacgctg acaacgcctc ttcgaacgat
     7681 cccatcccga cggtctgtgt t
//
LOCUS       DAOSOR010000254         7662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9554_length_7662_cov_3.997239, whole genome shotgun sequence.
ACCESSION   DAOSOR010000254 DAOSOR010000000
VERSION     DAOSOR010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7662
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9554_length_7662_cov_3.997239"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="PK710_13930"
     CDS             <1..1168
                     /locus_tag="PK710_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT10872.1"
                     /translation="DQAKSAAKALFDRLAPNDDFSLITFSTDAQVRVQNTRVAAAKAR
                     VHGAIDEIEATGGTNISEGLRLGYAQAKSGGIPADAVRVVFLLSDGRANSGITSPQRL
                     SQLALEAFQDGIQTSTFGLGTDYDGPLMSAIASDGAGGYYYLRDGQQIAPALSTELDR
                     RLDPAATAVEIRIRWSQGIDVLQVYGSRRLNEAEAARVRAIEVAADDQAAKRDRIKKD
                     RQDDTQGGMRFLIPAFARDDNHVMLFKIRVPAGVEPRQVALIELKYKDRGTKRNFSEE
                     VRVNLPYANSDAQSAQSIQPQVARTVQGFAAGETLMEAARFVSHGDRPRALALLTERE
                     QILRKAAESLQEPGFRRDADRLARFRSHLDTNAGVGESLVLAMLLETAGRAHLR"
     gene            1194..2048
                     /locus_tag="PK710_13935"
     CDS             1194..2048
                     /locus_tag="PK710_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10873.1"
                     /translation="MPSTTFPIRLDTPAVHVPIEPGGKLILRGYFHSTHDGSIVDAAT
                     TTWPSGAPGGASVDAAGLIDWQAGGFHLVSRDPQTHEVQLLATDGPAPACDLNHVQAP
                     CLPLRAHHQALSRLMTVSEWTSSLKGGIQGEVVAPPVYAPVTTIASTMKPVLIVSGVG
                     LLVLAMLLFVGWQFRRWSRSAPVELRRLAKRVRDKALRADPILSGPLAPALDSMIKAI
                     ESSKVDPVSEEGQRIVKVLRRVDATLDEKAAENKACDERKMADELVSEVEIALEAAGE
                     AARLGPQR"
     gene            complement(2119..3426)
                     /locus_tag="PK710_13940"
     CDS             complement(2119..3426)
                     /locus_tag="PK710_13940"
                     /inference="COORDINATES: protein motif:HMM:NF011655.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HOT10874.1"
                     /translation="MKFPGRRKYTHFFPVKARDPLLNRAGIETFSHRTHVVGIDQTLV
                     DIEAHVSDDFLQRHDLSKGHSLVVEAGRAEAIYAELSEYGLIMQEHAGGTVGNTLHNY
                     SVLADDNSILLGVMSESIHVGTSGYRYLCHTSSRVNLDHLQPVDGPIGRCFALITPDH
                     ERTFAINEGAMNQLRPQSIPAQAFEHASALLITAYLMRCKPSDPMPESALRAIALAKR
                     ARVPVVLTLGTKHVIADRPEYWKAFIRDNVNIVAMNEEEGMALTGHEDPLLACDATLD
                     LTDLVLCTAGPEGLYMAGHVDEKAKRQTRYPLLPGAIPEFNRYEFSRAMLREHCKSPV
                     RAFAYIAPYMGGPNRIANTNGAGDGALAAVLHDIAANRHHRENVPNSSKHTFQFLTYS
                     SLAQVCRYANRVSFEVLAHGSPRLTHGLPEREDSLKEEAYWAQ"
     gene            complement(3596..4612)
                     /locus_tag="PK710_13945"
     CDS             complement(3596..4612)
                     /locus_tag="PK710_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10875.1"
                     /translation="MRHWFWIIPATLLVGNLNACSSNEQSGNDSNSHPAGSGGADAST
                     DSGQVDTSVDDADASANPVDGQAESMGGSGGGSTTEDASDAGGPFKTGSVGLSQTAMT
                     VNGTEYVSYSFFAQFMRVTNDPENSPCEIQEQGDSRIVDCATSTIDGGTMAYEKVSAG
                     PVSLAGANIPLTLTPDATGTYEVRTGQERLWTKGASLTISAQGGEVPAFQTTLTGVGS
                     AVVSVPAAASGSTMTISTKEPLALAWSGAEGGTMTATLSRNDANKARSVTISCSFDGA
                     KTTGTVPASVMAMIPPGPLGSLVLMGGDNRRVDVSDWTIYVSETVVAVTTQGTSAAWS
                     VTFE"
     gene            4784..6118
                     /locus_tag="PK710_13950"
     CDS             4784..6118
                     /locus_tag="PK710_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10876.1"
                     /translation="MLGRSVKAIAILLVGMSAACSAGGGNSGVSGSHAGGNTLEEGGI
                     GGAGNASTGGSGGLLIPESGPVDATQHVATLQGRVVAPEGTIPISGALVYLAYSPPPP
                     IPDGVFCDECVKLDPDDEYTLTSPDGSFSLLAHSTGSRYLVVQKGQFRKVRPIQIEAG
                     DQQVDPEFTRLPARMNKAAGEDIPKMAIIVGKWDSIELSLAKLGLAKIVPGFLGSTVD
                     PATAGFDMIQGVFPPDLSDPMKNPTAFLKNYDYLKRYHIIFIPCSGSDGTTCTDTVPD
                     NKTVQDNLKRFVSEGGKLYVTDYSYEYVRRPWPGFVNWDTETSATGSACLSGEYDAPA
                     TVEDQEMREWLLAQGIDQFEVEANWTRISALHTLPGFDPKGAPIDVTPKTWVKGSQNP
                     ATISFEDRCGRVLFSTYHTEGSGESALLAQERALLYVLLEVSVCVGPPIPPK"
     gene            complement(6334..6606)
                     /locus_tag="PK710_13955"
     CDS             complement(6334..6606)
                     /locus_tag="PK710_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10877.1"
                     /translation="MTRSKLTATATLTGVSSWAFRKDSLSHIRALRSYHESSVRAESD
                     CRWPVEKKRSLDDTMRIDEDTLGTNVDAAGERFGQIPSFFLNWKGI"
     gene            6587..>7662
                     /locus_tag="PK710_13960"
     CDS             6587..>7662
                     /locus_tag="PK710_13960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOT10878.1"
                     /translation="MSLLLVTARFVLSESIGFGDAEALYASYSLFGQATYLDHPGLIG
                     WLGSWFLNEQGVADPVSIHRFTSIAATAIPWLGGLAARFAGASARGAILAVLAIAIVP
                     ETAIGLFAFSPDLPLAIAWLGALGLAALALRSHPSSLRSFAATLGAGLCTGLACAAKV
                     TGILLAIALVATWSSRPARERLRTFAPYFAVLLGIIVTLPMLQREISLGWPMLHHRLW
                     HTQVGFGLTLRNVGALLGGQALYLTPFVAIAIVAIAIDLARQPHNDAVDRLLFYTTIP
                     PFVALATLTILSRVAEPHWVAPAYLGLALHLGRRADSALVSRRLALASVFTSLLAIAV
                     VFLVVRFPVLPKILGDSYDPKLDIT"
BASE COUNT         1590 a         2370 c         2050 g         1652 t
ORIGIN      
        1 ggaccaagcc aaatccgcgg ccaaagcctt gttcgaccgg cttgctccca acgatgattt
       61 ttctctcatc acgttctcga ccgatgccca ggtgcgtgtg caaaacacac gcgtggcagc
      121 cgccaaagcg cgtgttcacg gtgccatcga cgagatcgaa gctacggggg gaaccaatat
      181 cagcgaagga cttcgtctgg gctatgcaca agcaaaaagc gggggaattc cagcagatgc
      241 cgtgcgggtg gtgttcctcc tttccgacgg tcgagccaat tccggtatca ccagccccca
      301 gcggttgtcc caacttgcgc tcgaagcttt ccaggacggg attcaaacca gcaccttcgg
      361 tctcggcacc gattacgacg gtcccttgat gagcgccatc gcttccgatg gtgccggcgg
      421 ctattactac cttcgcgacg gacagcaaat cgcaccggcc ctttccaccg aattggaccg
      481 acgcctggac cccgccgcta ccgccgtgga aatccgcata cgctggagcc agggaatcga
      541 tgtgcttcaa gtgtatggct cacggcgtct caatgaggcg gaagccgctc gggtgcgcgc
      601 gatcgaagtg gccgcggacg atcaagcggc aaagcgtgac cgcatcaaaa aagaccggca
      661 agacgatacg cagggcggga tgaggttttt gattcctgct ttcgctcgcg atgacaacca
      721 cgtgatgctg ttcaaaattc gtgtccccgc tggtgtcgaa ccacgccagg tggccctgat
      781 cgaactcaaa tacaaagacc ggggaacgaa acgaaatttc tccgaggaag tgcgtgtcaa
      841 cctgccatac gccaacagcg atgcccaaag tgcccaatcc atccagccgc aggtcgcacg
      901 aacggtgcaa ggattcgccg cgggcgaaac cttgatggaa gcggcccgat tcgtgagcca
      961 tggggaccgt ccccgcgccc tggccttgct caccgaacga gagcaaatcc ttcgcaaggc
     1021 cgcggaatca ttacaagaac ctggatttcg acgagatgcc gatcggcttg cgcgttttcg
     1081 ttcccatcta gacaccaacg ccggggtagg cgaatctctc gtgctcgcca tgcttctcga
     1141 aaccgcggga agggctcacc ttcgctgaca cttccatcgt aggctgatac accgtgcctt
     1201 ccacgacttt tcccattcgc ctcgacactc cagccgttca tgtacccatc gaacccggcg
     1261 gcaaactcat ccttcgtggc tatttccact cgacccacga tggctccatc gtggatgccg
     1321 cgacaaccac ctggccttcc ggcgcaccag gcggcgctag cgtggatgca gccgggctca
     1381 tcgattggca agctggtggg tttcacctcg tttcgcgcga tccccaaacc cacgaagttc
     1441 agttgctggc taccgatggt cctgcccccg cctgcgatct caaccacgtc caagcgccct
     1501 gcctgcccct tcgcgctcac catcaggccc tgagccgcct gatgaccgtt agcgaatgga
     1561 cctcatcgct caaaggcggc atccaggggg aagtggtagc gcctcctgtc tacgcaccgg
     1621 tcaccacgat cgcctccacc atgaagccgg tgctgattgt cagtggagtt gggctgctgg
     1681 tacttgccat gctccttttc gtggggtggc agtttcgacg ctggtcccgg tcagctccgg
     1741 tcgaattgcg aagattggca aaacgcgtgc gagacaaagc attgcgcgcg gatcccattc
     1801 tttcaggccc tcttgctcca gccctcgatt cgatgatcaa agccatcgaa tcgagcaaag
     1861 tcgatccggt gtccgaagaa ggacaacgca tcgtgaaggt gctgcgtcgt gtcgacgcca
     1921 cgctcgatga aaaggcggcg gaaaacaaag catgcgacga gcgaaagatg gcggacgaat
     1981 tggtttcgga agtggaaatc gcgctggaag cagcgggaga agcagcacgt cttggaccgc
     2041 aacgatgaca gccatcaaaa acggttatcg gagccgctcc tatcatgtcc gaccccgacg
     2101 actccgcatg tagtctgatc attgagccca ataggcttct tctttcagac tatcctcgcg
     2161 ttccggcagc ccgtgggtca gccgtggtga accatgggcc agcacttcga agctaacccg
     2221 attggcgtac cgacaaacct gcgcaagcga cgaataagtc agaaattgaa atgtatgttt
     2281 cgatgaattc ggcacgtttt cgcgatgatg ccgattggca gcgatgtcgt gcaacaccgc
     2341 agccaatgcg ccatctcccg ctccattggt gttggcgatc cggttggggc cacccatgta
     2401 gggagcgata tacgcaaagg cgcgcaccgg tgatttgcag tgttctcgca gcatcgcgcg
     2461 actgaattca taacggttga actcgggaat ggcgccagga agcaggggat aacgtgtctg
     2521 ccgtttcgcc ttctcatcca cgtgtcccgc catgtacagc ccttccggac ccgccgtgca
     2581 aagcaccaaa tccgtcagat ccaaggtggc gtcacacgct agcagtgggt cttcatgccc
     2641 tgtcaacgcc atcccttctt cctcgttcat cgccacgatg ttcacgttgt ctcgaatgaa
     2701 tgccttccag tattccggtc gatcggcaat cacgtgtttc gtgcccagcg tgagcaccac
     2761 cggaacccgc gctcgttttg ccaaagcgat ggcccgcaac gcggactccg gcatgggatc
     2821 ggacggtttg cagcgcatga gatacgcggt gatcagcaat gccgatgcat gctcgaacgc
     2881 ttgcgcagga atgctttgcg gacgaagttg gttcatcgca ccttcgttga tcgcgaaggt
     2941 gcgttcatga tcgggcgtga tcagcgcaaa acaccgacca atcggaccat ccacgggctg
     3001 caggtggtcg agattcacgc ggctcgaggt atgacaaaga taccgatacc ctgaggtgcc
     3061 cacgtgaatg ctctcgctca tcacccccaa caggatcgag ttatcgtcag caagcaccga
     3121 gtagttgtgc aaggtattgc ccaccgttcc ccccgcatgc tcctgcatga tgagtccgta
     3181 ctcggatagt tccgcgtaaa tcgcctccgc acgaccggct tccaccacca acgaatgccc
     3241 cttgctcaaa tcatgacgct gtaggaaatc gtcggataca tgagcctcga tatcgaccaa
     3301 agtctgatcg atacccacga catgcgtacg atgagaaaac gtctcgatcc ccgcccgatt
     3361 gagcaaggga tctcgcgctt tgacggggaa aaaatgggtg tacttgcgac gtccgggaaa
     3421 tttcatggcg cccttggcga aaagaaaccc gcaccctact gtcagcgatc gtcgctttca
     3481 acctccgtcc cgcctgtcgc cctcccaggc acgacgaatt taatagaacg aagggggaga
     3541 gaaaaacata cggacggtct accgcgcttg cctgcgaaac gaacgcttcg ctccgtcact
     3601 cgaacgtcac cgaccacgcc gcggacgttc cttgggttgt cacggctacc acggtctcgc
     3661 ttacgtaaat cgtccaatcg gatacatcga cgcgccgatt atcccccccc atcaagacca
     3721 aggagccaag aggcccgggc ggaatcatcg ccatgacgga agctggcacc gtgcccgttg
     3781 tcttcgcgcc gtcgaacgaa caagaaatag tcaccgatct tgccttgttt gcatcattgc
     3841 gagaaagagt agcggtcatg gtccctccct ccgctccaga ccacgcaagc gccaatggct
     3901 ctttggtgga aatcgtcatg gtcgatcccg atgcagcggc cgggaccgaa accacggccg
     3961 acccaacgcc cgtgagtgtg gtctgaaacg ccgggacctc gccgccttgc gcggaaatgg
     4021 tgagcgatgc ccctttcgtc cacagacgct cctggcctgt ccgcacctcg taggttcccg
     4081 tcgcatccgg tgtgagcgtc aagggaatat tggcccccgc aagcgatact ggacccgcac
     4141 tcactttctc gtaagccatg gtgcccccat cgatcgtcga agtcgcgcag tccacgattc
     4201 gggaatcccc ttgttcttgg atttcgcaag gtgaattctc cgggtcgttc gttacccgca
     4261 tgaattgtgc gaaaaacgaa tagctaacgt attccgtccc gttgacggtc atcgccgttt
     4321 gggacaagcc cacgcttccg gttttgaaag gtcctcccgc gtcactggcg tcttcggtgg
     4381 tgcttccccc gccggaacca cccatgcttt cggcctggcc atctacagga ttcgcgcttg
     4441 catccgcatc atccacactc gtatcgacct gtcccgaatc ggtggaagca tccgctccgc
     4501 cgctgcctgc tggatgcgaa ttcgagtcgt ttccgctttg ctcattgctg gaacaagcgt
     4561 tcaaattgcc aaccaatagt gtcgctggaa tgatccagaa ccaatgtcgc attgaaaact
     4621 ctccctgtgc atcgtggatg atgaccgaat gaatggctgg tgtcctacct tttctgatca
     4681 ccacctcgtt caaccgcaag gttagaaaac aaccaacgcg gcttggcctc tgcggatggc
     4741 ctgcgctatg atgatttgta ttttgcggcc tgtggaggtg accatgctag gtcgttcggt
     4801 caaggcaatc gctatccttt tggtgggaat gtctgctgcc tgttccgccg gagggggcaa
     4861 ctccggagta agtggctccc atgccggggg aaacaccctt gaggaaggag ggataggcgg
     4921 tgcgggtaat gccagcacgg gaggctccgg cggcttgctc atccctgagt cgggccccgt
     4981 ggatgcgact cagcacgtgg ccacgctcca aggacgagtc gttgcccctg agggcacgat
     5041 tcccatctcg ggcgcgttgg tgtacttggc ctattcacct ccaccaccga ttccagatgg
     5101 cgtattttgc gatgaatgcg tcaagttgga tcccgacgac gaatacacgc tgacgagtcc
     5161 ggatggttcg ttctcgcttc tggcccattc gacgggctct cgatacctcg tggtgcagaa
     5221 aggccaattc cgaaaggttc gacccatcca aatcgaggct ggtgatcagc aggtagaccc
     5281 agagttcacg cgtcttcccg cgagaatgaa caaggccgct ggagaagaca tccccaaaat
     5341 ggcaatcatc gtgggtaaat gggattcgat cgagctatcc ctcgccaaac taggtttggc
     5401 gaaaatcgtt cctggattcc tcggttcgac ggtcgacccg gctaccgccg gattcgacat
     5461 gattcagggc gtgtttcctc cggatctatc cgatccgatg aaaaacccaa cggcgtttct
     5521 gaagaattac gactacttga aacgctatca catcattttc attccttgtt ctggaagcga
     5581 tggcacgacg tgtacggata cggtgccgga caacaaaacc gtacaagaca atctcaagcg
     5641 tttcgtcagc gaagggggaa agctgtatgt cacggattat tcttatgaat acgtgagacg
     5701 gccgtggccg ggctttgtga attgggatac ggaaacctct gccaccggtt ccgcatgcct
     5761 ttcgggcgaa tacgatgctc ccgccactgt cgaggaccag gagatgcgtg agtggttgct
     5821 cgcccagggg atcgatcaat tcgaagtgga agcgaattgg acgagaatat ccgcccttca
     5881 cacccttcct ggcttcgacc cgaaaggggc tcccatcgat gtcacaccca aaacctgggt
     5941 gaaaggcagt caaaatcccg cgaccatcag cttcgaagac cgttgcggac gcgtcctttt
     6001 ttccacctat cataccgaag gaagtgggga aagcgcgttg ctcgcccaag aacgtgcctt
     6061 gctttacgta ttgctcgagg tcagcgtttg tgtcggtccc cccatcccgc cgaagtaaac
     6121 actccctgag ccatcccctc atcttttctc gagcctttcc ttcacgatca cagcccacgg
     6181 tcacggcgca cggcacacaa aaacgctcac ggtcatccca tatcgttcac cgcaaaacgc
     6241 cagcccaatg aataatcgcc cagggcaact gttgggggtg ctcaaacggg ggctaaagga
     6301 ccgagcccaa atcaagaccc aagaatcttc agctcatatc cctttccaat tcaggaaaaa
     6361 tgaggggatc tgtccgaaac gctcccctgc cgcgtccacg ttggttccca acgtgtcttc
     6421 atcaatccgc atcgtatcgt cgaggcttcg tttcttttcg acaggccaac ggcaatcgga
     6481 ctccgcacga accgatgatt cgtgatagga gcgcaacgca cgaatgtggg aaagactatc
     6541 cttccgaaat gcccaagacg acacgcctgt caaggtagcg gtggcggtga gtttgctcct
     6601 tgtcacggcg cgcttcgttc tgtcggaaag tatcggtttc ggtgatgcag aagcacttta
     6661 cgcttcctac tctcttttcg gacaggccac ctacctggat catcccggcc ttatcggatg
     6721 gctcggttct tggtttttga acgaacaagg cgtcgctgat cccgtctcga tccatcgatt
     6781 caccagcatt gccgccacgg caatcccttg gctgggtggg ctcgcagctc ggttcgccgg
     6841 tgcatctgcc cgaggagcca ttcttgctgt tcttgccatc gccatcgtgc cagaaaccgc
     6901 catcggattg ttcgcgtttt ccccagacct tcctctcgcc atcgcatggt tgggagcgct
     6961 tggtctggca gcgttggcgt tgcgctcaca cccctcttcc cttcgaagct tcgccgctac
     7021 tctcggcgca ggcttatgca ccggcctcgc ctgcgcagcc aaagtcacgg gcattctcct
     7081 tgccatcgct ctggtagcca cctggtcctc ccggcccgca cgcgagcgtc tgcgcacatt
     7141 cgccccctat ttcgctgtct tgctcggcat catcgtcact ttgcccatgc tacagcgaga
     7201 gatatccctg ggatggccca tgctccatca tcgcctgtgg cacacccaag tcggtttcgg
     7261 acttaccctg cgcaacgtgg gcgcattgtt gggggggcaa gctctttatc ttaccccctt
     7321 cgtcgccata gccatcgtgg caatcgcgat cgatctggcc cgacaaccgc acaacgatgc
     7381 ggtggaccga ttgctttttt acacaaccat cccacctttc gttgcccttg cgacgctcac
     7441 catcttgtcg cgcgtggcgg aaccccattg ggtcgcgcct gcctaccttg gcctggcttt
     7501 gcaccttgga cgacgagccg atagcgcgct tgtgtcgcgt cgactcgccc ttgcctccgt
     7561 gttcacctcg ctgctcgcca tcgccgtggt tttcctcgtc gtccggtttc cggttttacc
     7621 gaaaattctc ggtgattctt acgatcccaa gctggacatc ac
//
LOCUS       DAOSOR010000255         7646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9581_length_7646_cov_4.345804, whole genome shotgun sequence.
ACCESSION   DAOSOR010000255 DAOSOR010000000
VERSION     DAOSOR010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7646
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9581_length_7646_cov_4.345804"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PK710_13965"
     CDS             complement(<1..224)
                     /locus_tag="PK710_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10879.1"
                     /translation="MQRKQSYSWLAASLGAAFVLFAGACGGDDSDSPPQGKPGVSGSG
                     GGAGEAGMAGEAGTSGGGTGGEETGGGGTGG"
     gene            complement(580..3345)
                     /gene="ppdK"
                     /locus_tag="PK710_13970"
     CDS             complement(580..3345)
                     /gene="ppdK"
                     /locus_tag="PK710_13970"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOT10880.1"
                     /translation="MMSKYVYFFGGNKADGKAAQKNLLGGKGANLAEMVNIGIPVPPG
                     FTLTTEVCTEYFKNGGKFPAELEAQVNEALAKTEQLLGKKYGDPTNPLLFSVRSGARA
                     SMPGMMDTVLNLGLNDEIAAGLAKKTGNEKFVWDAYRRFITMYSDIVLEVSKEHFEAD
                     LEASRREVATRKGIDVTKHNAEELKTLVPDSELGAEDFRKLVGVFKEIAKKHTGKPFP
                     TDPKEQLWGAIRAVFGSWNNQRAKTYRKMHDIPEEWGTACNVQAMVFGNLSDASATGV
                     AFTRDPSTGENVFYGEWLPNAQGEDVVAGIRTPFNVTKAAAEKGGKPSSSLEEKMPQA
                     YKILEGIYHKLEQHYHDMMDIEFTIEEGTLFMLQCRVGKRTAAAAVRMAVEMHKEGLI
                     SKEEAVLRVDPASIDQLLHPTLDPKAPKKTLATGLPASPGAASGMVVFCADEAEKQAA
                     EGKKVLLVRIETSPEDIHGMHAAQGILTARGGMTSHAAVVARGMGKCCVAGVSALNID
                     YDANQMQITVYEGKTAVETVTVKQGDFLTLDGSAGKVYQGEVPTIEAQLTGYFGELMQ
                     WADEVRTLKVRTNADTPHDAQVARKFGAQGIGLCRTEHMFFGDDRIAVVREMILSDTV
                     EQRKKALDKLLPFQREDFIGIFKAMDGLPVTIRTLDPPLHEFLPNKAEQIAELAKTMG
                     VSQDKLHHKVEELHEFNPMLGHRGVRLAISYPEIASMQMRAIIEAAVACANEGVDVQP
                     EVMIPLAMTKRELDLMKAEFDAIAKEVLPKAKKPVNILFGTMIELPRAALCADELAKT
                     AEFFSFGTNDLTQTTMGLSRDDAGRFLDEYVKTTVFAKDPFQTLDVEGVGQLVRTAVE
                     RGKQTRPDIKLGICGEHGGDPASIHFCYQVGLAYVSCSPFRVPIARLAAAQAALVDKG
                     GKVASTA"
     gene            complement(3351..5432)
                     /gene="glyS"
                     /locus_tag="PK710_13975"
     CDS             complement(3351..5432)
                     /gene="glyS"
                     /locus_tag="PK710_13975"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOT10881.1"
                     /translation="MSDTENLLFEIGVEELPASYVERALIVLPDLFEERLRQARLSHT
                     GIRALGTPRRLAVQVYDLALRQPDLSERVQGPPVQVALDKEGKPTKAALAFARKIGCE
                     VDDLERVDSGKGVYLMGTRQEEGKPTIELLPRILEEICAAIPFRKSMRWANGDIAFGR
                     PVRWILALLGGQVVDMSFAGVRSGRETMGHRFLAPEPIDLPDPQDYVELLRDAHVIVD
                     PFERRDVMMSQLREACEEAGTVLVEDEFLERENVNLVEEPQVITGSFDEAFLALPEDV
                     ILAVAKGHQRYFCARREDGELAPMYLAVAGTALKPDMVRRGNDRVMRARLSDARFFFE
                     EDMKVRLDSRFERLGGIVFQKRLGSVRDKVERMERLVVRLGRSLGLSEDRIETAQRGA
                     HLCKCDLASLMVGEFPELQGEMGAVYALGQGETEAVARVISKHYQPKGASDATADDDP
                     AALVALADRFDTLVGCFSVGLSPTGAADPFALRRACLGVLRTALDRGFALSIREAIEV
                     AYEGVEGLEVDLPMGEVVSKLLEFFRERLRGLLVEHHPHDVVDACLASGFDNPVDVRA
                     RVVALAGLEPSVRTRAGEVFKRATNIAKEAPSGAPRDPASMGESVHEAEIATFRGLED
                     LDVALQNAHNRRDFPAAFAAIAAFAPLLAEFFTHVLVMDEDKVLRNNRLRLMRTISER
                     CSGIAHFNMLT"
     gene            complement(5586..6362)
                     /gene="rph"
                     /locus_tag="PK710_13980"
     CDS             complement(5586..6362)
                     /gene="rph"
                     /locus_tag="PK710_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOT10882.1"
                     /translation="MDDNDESVKKDPRKIRTVQIIPHFHRLAEGSVLYKAEGTVVLVT
                     ASIDENVPEFMRGRGVGWITAEYQMHPRANPSHREKRDGRGKAISGRTQEIQRLIGRS
                     LRAAIQPDRLGERTITIDCDVLEADGGTRTASITGAWVALVLALSTLHDRGVLTKPVL
                     RDQVAAVSVGHVDGQHVLDLCYAQDSRARVDLNLVATGRGAIVEIQGTAEGEAIPRAQ
                     MDALVDLALEGIGMLMDVQREVLEQAGIDLSALMIRDEGV"
     gene            complement(6402..>7646)
                     /locus_tag="PK710_13985"
     CDS             complement(6402..>7646)
                     /locus_tag="PK710_13985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT10883.1"
                     /translation="LRPRIFERIRQSATSKVLTSTDGKARQMSDQIVAASGELEAFLA
                     EDRAMLASLFEALETADAPRAKVLRDGVMAHEIAGARRTRVLKEQVESEMDQRIAAAR
                     DRERRALHSLLLLAGLTLLVGFGMTLYARRVLRPLAAVTDRARAVAAGDLTSQKVVAA
                     RDEIGELAATFESMVEAISKANEARLQSERLAAVGRMAAHVTHEIRNPLSSIGLNLEL
                     LEDELAPVNGSDEPKQLLQAIRRQVDHLSALSEEYLRLARPPQSRLEAEGLPEMVREV
                     SSFVRPELDKAGIELNLELPEQASDVWLDEQQIRQVLVNLIRNAREAMSKGGKLRIAM
                     HEFEQWAELWVEDTGPGIADDVRMNIFDPFYTTKNRGTGLGLAITRQVIDSHGGSIHC
                     EPVEPHGTRFRIRLRRADVADR"
BASE COUNT         1672 a         2619 c         1832 g         1523 t
ORIGIN      
        1 cctcccgttc caccccctcc cgtttcttca ccacccgttc cccctccact ggtcccagcc
       61 tccccagcca tccctgcttc ccccgcgcca ccgcccgagc cagacacccc tggtttgcct
      121 tgcggagggg aatccgaatc atcccctccg caggcccctg cgaaaagaac aaacgccgcg
      181 cccagcgagg ccgcgagcca tgaatacgat tgcttgcgtt gcatcaagac ctccaaagca
      241 tccacacgtg ttgccaaacc acagcagcaa gaaaggtcca tccacgaatc ttcatcgcct
      301 gtggaaccat cgccaaatcc ctcaaatcgg ggatgtgcga acgatcgtag cgcgtttgct
      361 caaggcttgc cgttacaaag gtgggccccc tcaccgtttg ccaaaccccc gagcgggtaa
      421 cgaccttacc tttgcgcgat agggagccaa agacgcacga ttgaggggcg aaaaccatcg
      481 ccggacaata gtcaagtcat tgaattaact agttaaatac aggggtcgaa ggacggcagg
      541 gggggctggc gggttggacg ggagaaggga aaggcagtgt tacgcggtgc tggctacctt
      601 gcctcccttg tccaccaaag ccgcttgcgc cgctgcaaga cgcgcgatcg gaacacggaa
      661 gggagaacaa gacacataag ccaaccccac ctggtagcag aagtgaatcg aagccgggtc
      721 accaccatgc tcaccacaaa ttccgagctt gatgtccggt cgggtttgct tgcctcgctc
      781 cacggctgtt cgaacgagct gccccacccc ctcgacatcg agagtctgga atgggtcctt
      841 cgcgaagacc gtcgttttca cgtactcgtc caaaaaccgc ccggcgtcgt cgcgagacaa
      901 ccccatcgtg gtttgcgtca agtcgttggt gccaaacgag aagaattcag cggttttcgc
      961 aagctcatcg gcacaaaggg ccgcgcgcgg cagttcgatc attgtcccga aaaggatatt
     1021 gaccggcttt ttggccttgg gaagtacttc cttcgcaatg gcatcgaact cagccttcat
     1081 caggtccagc tcgcgcttgg tcatcgccag cggaatcatc acttccggtt gcacatcgac
     1141 gccctcattc gcacaagcga cagccgcttc gataatcgca cgcatctgca tggacgcaat
     1201 ttcgggatag ctgatggcaa gacgcacacc gcgatgtcca agcatcgggt tgaactcatg
     1261 caactcctcg accttgtgat ggagcttgtc ttgagacaca cccatcgtct tcgccaactc
     1321 ggcgatttgc tccgccttgt tgggaaggaa ctcatgcaaa ggcggatcga gcgttcgaat
     1381 cgttaccggc aggccatcca tggccttgaa aatgccgatg aagtcctcgc gctggaacgg
     1441 aagcagctta tcaagagcct ttttccgctg ctccaccgta tcgctgagga tcatctcccg
     1501 caccaccgca atgcggtcgt cgccaaagaa catatgctcg gtacggcaca acccaatccc
     1561 ctgcgcccca aacttacgag ccacctgcgc atcgtgaggc gtatccgcat tcgtacggac
     1621 tttgagcgtt cgcacctcat cggcccactg catcaactca ccaaaataac ccgtgagttg
     1681 cgcttcgatc gtcggcacct cgccttgata gaccttgcct gccgaaccat cgagggtcag
     1741 gaagtctccc tgcttgaccg tcaccgtctc cacggcggtc ttgccctcat acaccgtgat
     1801 ttgcatttga ttggcgtcgt aatcgatgtt cagagcggaa acgcctgcga cgcagcactt
     1861 gcccatgccc cgggccacca ccgccgcatg gctggtcatc cctccacgcg cggtcaaaat
     1921 gccttgcgcc gcgtgcatgc cgtgaatgtc ctccggcgag gtttcgatgc gaaccaacaa
     1981 caccttcttg ccttcggccg cttgtttctc cgcctcgtcc gcgcagaaca ccaccatccc
     2041 actggcagct ccagggctgg cgggtagacc cgtagctaac gtctttttgg gggctttcgg
     2101 gtcaagggta ggatgcaaaa gctgatcgat ggaggcagga tcaactcgca acaccgcttc
     2161 ttctttcgag atgagcccct ctttgtgcat ctcgaccgcc atgcgcaccg ccgcggccgc
     2221 cgttctcttt cccacacgac actgcaacat gaacaacgtg ccctcttcga tcgtaaactc
     2281 gatgtccatc atgtcgtggt agtgctgctc gagcttgtga tagatgccct cgagaatctt
     2341 gtaagcctga ggcatttttt cctcgagcga tgagctaggc ttgccacctt tttccgcggc
     2401 cgccttcgtg acgttgaaag gcgtgcgaat gcccgccacc acgtcttctc cctgcgcgtt
     2461 gggaagccat tcgccataaa acacgttttc gcccgtggat gggtcgcgcg taaacgcaac
     2521 gcccgtcgcg ctcgcatcac tcaagtttcc aaagaccatc gcctgtacgt tgcaggcagt
     2581 cccccactcc tcggggatgt cgtgcatctt gcgataggtc ttcgcacgct ggttgttcca
     2641 actgccaaac accgcgcgga tcgcgcccca aagctgctct ttcggatcgg taggaaatgg
     2701 cttgcccgta tgctttttcg caatctcctt gaatacaccc accagcttgc ggaaatcctc
     2761 cgccccaagc tccgaatccg gaaccaaggt cttgagttct tccgcgttgt gcttggtcac
     2821 atcaatgcct ttgcgcgtgg ccacttcacg acgcgacgcc tcgagatccg cttcgaagtg
     2881 ctctttgctc acctcgagaa cgatgtccga gtacatggtg atgaaacgac gataggcgtc
     2941 ccatacgaac ttttcattgc ccgttttctt ggcaagtccc gccgcaatct cgtcgttgag
     3001 accaagattg agcaccgtat ccatcatgcc tggcatcgaa gcccgagcac cgctgcgcac
     3061 actgaagagc aacggattgg tgggatcgcc atattttttc ccaagcaact gctcggtctt
     3121 cgccaaggcc tcattgacct gggcctcgag ttccgcgggg aatttaccgc cgtttttgaa
     3181 gtactccgtg cagacctccg tggtcagggt gaagcctggc ggcacaggaa tcccaatgtt
     3241 caccatctcc gccaggttgg ctcccttgcc accaagcagg ttcttttgcg cagccttgcc
     3301 atcggctttg tttccgccga aaaaatatac atacttggac atcatcgacc tcacgtgagc
     3361 atgttgaagt gagcgatacc cgagcaacgc tcggaaatgg ttctcatcag acgcagcctg
     3421 ttgtttctca acaccttgtc ctcgtccatc accaggacat gcgtgaaaaa ttcggcaagg
     3481 aggggagcaa aggccgcgat ggcggcaaag gccgcgggga aatctcgcct gttgtgcgca
     3541 ttttggagcg cgacgtcaag gtcttcgagg cctcgaaagg ttgcgatttc cgcttcgtga
     3601 acgctctcgc ccatcgaagc gggatcccgc ggggccccgg atggcgcctc tttcgcgata
     3661 ttcgtcgctc gtttgaatac ttcccccgca cgggtccgta ccgagggctc caatcccgcc
     3721 aacgctacca cccgcgcccg aacgtccacc ggattgtcaa agcccgacgc caaacacgca
     3781 tccaccacgt cgtgaggatg atgctccacc aacaatccgc gcaaacgctc ccgaaaaaat
     3841 tccagcagct tcgaaacgac ctcacccatc ggcaagtcca cctcgaggcc ctccaccccc
     3901 tcatacgcaa cctcaatcgc ctcccgaatc gataacgcaa acccccgatc gagagccgtg
     3961 cgcaataccc ccaaacaggc gcgacgcagc gcaaaaggat ccgcagcccc cgtgggactc
     4021 aaccccacgg aaaaacaccc caccaaagta tcaaaccgat cggccaacgc caccaaagcc
     4081 gcgggatcgt catccgcggt cgcatccgaa gcccccttgg gctgataatg cttcgaaatg
     4141 actcgcgcca cagcctccgt ttccccttgc cccaacgcat acaccgcccc catctccccc
     4201 tgcagctccg gaaactctcc caccatcagc gatgccaaat cacacttgca aagatgcgcc
     4261 ccgcgctgcg ccgtctcaat ccgatcctcg gacaggccaa gcgatcgccc caaccgcacg
     4321 accaatcgct ccatccgctc caccttgtcg cgcacgctgc caagccgctt ctgaaaaaca
     4381 atgcccccaa gccgctcgaa acgcgaatcc aaacggacct tcatatcctc ctcaaagaaa
     4441 aatcgcgcgt cggacaaacg cgcccgcatc acccgatcgt tgccccgacg caccatatcg
     4501 ggcttcaacg cagtacccgc tacggccaaa tacatcggcg ccaactcccc atcctcacga
     4561 cgcgcacaaa aatacctttg atgccccttc gccaccgcca gaatcacatc ctccggcaac
     4621 gcaagaaaag cctcgtcgaa ggaacccgta atcacctgcg gctcctcgac caagttcaca
     4681 ttctcccgct cgagaaactc atcctccacc aacaccgtgc ccgcttcctc acaggcctcc
     4741 cgaagctgac tcatcatgac atcccgacgc tcgaacggat ccacgatcac atgcgcgtca
     4801 cgcaataact cgacgtaatc ctgcggatcg ggaaggtcga tgggctcggg tgccaagaaa
     4861 cgatgcccca tcgtctccct gccactgcgt accccggcaa aggacatatc caccacttgt
     4921 ccacccaaaa gcgcaaggat ccagcgcaca ggacgaccaa aagcgatgtc cccgttggcc
     4981 catcgcatgg atttgcgaaa aggaatggcc gcacaaattt cctcgagaat tcggggaagt
     5041 agctcgatcg tgggctttcc ttcctcctgc cgcgtgccca tcaaatacac acccttgccc
     5101 gaatccacac gctccaggtc atcgacctca caaccaatct tgcgcgcaaa ggccagcgcc
     5161 gccttggtcg gcttgccctc cttgtccaac gcaacctgca ccggaggtcc ctggaccctc
     5221 tcactcaaat ccggctgccg taacgcaaga tcataaacct gcaccgccaa tcgcctcggc
     5281 gtacccaacg cacgaatccc cgtatgcgat agcctcgctt gccgcaaccg ctcctcaaaa
     5341 agatccggaa gcacaatcaa agccctctcc acatacgacg caggaagctc ctcgacgcca
     5401 atctcgaaca gcaaattctc cgtgtctgac atgcagtctc gcctccaatg gcaacgtgcg
     5461 gcggcgccaa cctaacaaat caccgcccca tgggcatctc cgattgcatc cccccatcca
     5521 ttctataacg caatgcgtta taacctcaaa acaacgcacc tacccccgtc gacaatccaa
     5581 aaccatcaca ccccctcatc ccgaatcatc aacgcggata gatcgattcc cgcctgttcg
     5641 agaacctcgc gctgcacgtc catgagcata ccaatgcctt cgagggcaag atcgacgagc
     5701 gcgtccatct gggcgcgggg aatggcttct ccttccgccg tgccttgaat ctccacgatg
     5761 gcgccgcgtc ccgttgccac aaggttgaga tccacccgcg cacggctgtc ctgcgcatag
     5821 caaaggtcca aaacatgctg gccgtcgaca tgccccacgc tcaccgcggc aacctgatcg
     5881 cgcaacacgg gtttcgtcaa cacgcctcgg tcgtgcagcg ttgacaatgc caggaccagc
     5941 gccacccaag cccccgtgat cgaggcggtt cgcgtgccgc catcggcttc gagaacatca
     6001 caatcgatgg ttatggttct ttctccaaga cgatccggtt ggatcgccgc ccgaagggat
     6061 cgaccgatga gccgctggat ttcctgcgtt cgaccggaaa tggctttgcc tcgcccatcg
     6121 cgcttttcgc ggtgagaagg gttggcccgc ggatgcattt ggtactccgc cgtgatccat
     6181 cccacacctc gaccccgcat aaactccggc acgttttcat cgatcgatgc ggtaaccagc
     6241 accacggtgc cttccgcctt gtaaagcacg gagccttccg caagacgatg aaagtgcggg
     6301 ataatctgca cggtccgaat ctttcgggga tcttttttta cactctcgtc gttgtcatcc
     6361 atggtccttc acctccgcgg cctgccgaaa aaccttgacg atcacctatc ggcgacatcc
     6421 gcgcgccgca atcgaatccg aaaacgagtc ccatgcggct caacgggctc gcaatgaatc
     6481 gagccgccat ggctgtcaat cacctgcctc gtgatcgcga gtccaagccc cgtgccgcga
     6541 ttttttgtag tgtaaaatgg atcaaagata ttcattcgca cgtcgtccgc aatgcccggc
     6601 cccgtatcct ccacccacaa ctcggcccat tgctcgaact catgcatcgc aattcgtaac
     6661 ttccccccct tgctcatcgc ctctcgtgca ttgcggatca agttcaccaa cacctgacga
     6721 atctgctgct cgtctagcca cacgtccgac gcttgctccg gtagttcgag gttcaattcg
     6781 atccccgctt tgtccaattc cgggcgaacg aaactcgaga cttctcgaac catttcgggc
     6841 agtccttcgg cttccaaccg ggattggggc gggcgagcca aacgcaagta ttcctccgac
     6901 aaggcgctga gatgatccac ttgtcgacga atggcctgaa gcaattgctt cggctcgtcg
     6961 gaaccgttca ccggcgccaa ctcgtcttcc aacagttcga gattcaatcc gatagacgat
     7021 aaagggttgc gaatctcatg ggttacgtgg gctgccatgc gccccacggc cgcaagacgc
     7081 tcggactgca aacgagcttc gttggctttc gaaatcgcct ccaccatcga ctcgaacgtg
     7141 gctgccaatt ccccgatctc atcacgagca gccaccacct tctgcgatgt caaatcaccc
     7201 gcggccaccg ccctcgcacg atccgtcact gcggccagcg gacgaagtac ccggcgcgca
     7261 tacaaggtca tcccaaaccc taccaaaagc gtcaatcccg cgagaagcaa caagctatgc
     7321 aaggctcggc gctccctgtc ccgagcagca gcaatgcgtt gatccatttc ggactcgacc
     7381 tgttctttga gcaccctcgt tcgacgcgcc cccgcgattt cgtgcgccat gaccccatca
     7441 cgcaggactt tggctcttgg agcatcggcg gtttcgagcg cctcgaatag ggaagccaac
     7501 atggcccggt cttccgcaag aaatgcctcc aactcccccg aagccgccac gatctgatca
     7561 ctcatctgcc gcgctttgcc gtcagtcgac gtaagcacct tcgaagtcgc agactgacga
     7621 attctctcga aaatacgcgg acgtaa
//
LOCUS       DAOSOR010000256         7634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9597_length_7634_cov_4.139992, whole genome shotgun sequence.
ACCESSION   DAOSOR010000256 DAOSOR010000000
VERSION     DAOSOR010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7634
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9597_length_7634_cov_4.139992"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1438)
                     /locus_tag="PK710_13990"
     CDS             complement(<1..1438)
                     /locus_tag="PK710_13990"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HOT10884.1"
                     /translation="MGILLCVANLQCHFDRADRWDDTPPLAQPSCEPGTMRCQAGLQR
                     CDRIDGRTRWNIIDDCASRGLVCAPSLLSCVACLPSARDCDEQTVLVCRQDGSGFDPA
                     GFCDTTDGYACRNGLCTHLCSQASVVKSNVGCEYWAADLDNANVSPDSNAAAQQFAVV
                     VSNPQSDLFASVVVELDDAPVGAPSQPRTVGEASIPPYGLHVFLLGPREVDGSAHGSF
                     DTGPGTALTRQAYRVQSHVPVVAYQFNPLDNVNVFSNDASLLKPVEALPGSGLMELGY
                     VVLGWPQTIAITDDPDTNFNPVSPIQLQAFVTMIGTRPDTHVRFTSATHVVAGENIGP
                     LAPADTLEVVLQPFDVLNLETGDFLSDFTGSLIESDQPIVVFSGAEAADAPHFKRLAD
                     RSCCADHLEEQLDPLRTAGTTFIAPHSPNRSKAVAAAGALISVVPEPEYYRVLALSAE
                     GPTTIVTNLPEPDDQWVLPSRGAYVDLVS"
     gene            complement(1547..1837)
                     /locus_tag="PK710_13995"
     CDS             complement(1547..1837)
                     /locus_tag="PK710_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10885.1"
                     /translation="MKLHLHQFGNHRLRCQFEDPETARALWNEFIPAVLFHVFDIKRL
                     EPNTELPVLHGQGFVFQNKLLTAFGEKFDVVIVDSEGLKSLANRVTSDGSTN"
     gene            complement(1989..2255)
                     /locus_tag="PK710_14000"
     CDS             complement(1989..2255)
                     /locus_tag="PK710_14000"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOT10886.1"
                     /translation="MIGKKYIVRGRVQRVGFREFVRKKAVSLGIIGSARNLPDGTVAI
                     HAGGVPAALEAFEIELHRGPRLSRVDTVEGSPADLPTDGTFTTR"
     gene            2374..2673
                     /locus_tag="PK710_14005"
     CDS             2374..2673
                     /locus_tag="PK710_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10887.1"
                     /translation="MPSSTRLRARSPKPRAHATTPNYPRQSILVGVAMSLTACMGAAP
                     GPYAPATPSQTVTVKSEDSRFAQPPPDDVVEDTAQPVPPPDHDIPPPGEAPGPFE"
     gene            complement(2831..3508)
                     /locus_tag="PK710_14010"
     CDS             complement(2831..3508)
                     /locus_tag="PK710_14010"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOT10888.1"
                     /translation="MSDDPTSHSSVHPDEGVSDFEYPCHHTVGVGPHPLPWPEDDRLD
                     PEYLRNGDRRNVLDNFRYWKNEAIVAVLDTRRNPLHVAIQNWEHDFNIGSIVRTANAF
                     NVAGVHIVGRRKWNRRGAMVTDRYLTVHHHADEPQFFSWLRDRQIVPIGVDNFENAVP
                     LETFRLPRDCCLIFGSEGPGLTPRMVAGCEQLVAISQYGSTRSVNAGAAAAITMYHWS
                     LQHLGPS"
     gene            complement(3902..4312)
                     /locus_tag="PK710_14015"
     CDS             complement(3902..4312)
                     /locus_tag="PK710_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10889.1"
                     /translation="MTSLAHRHASPNMIGYHSYLPRAKTLMEPFRQQPQPKEQLLAPE
                     RLHAQNEALARYVDSARRKAFGRNDKYSIAATPDNIQIPWRYVLPIIVGFIVGRMLPT
                     FHVSFERSLLIGLLSALGLFAVAFLQDVIRWIRR"
     gene            complement(4379..4714)
                     /locus_tag="PK710_14020"
     CDS             complement(4379..4714)
                     /locus_tag="PK710_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10890.1"
                     /translation="MASLFARILARPRVNPTPSPFEIVARAAPGPNTVIRFVDEPFRV
                     SPLSVLIVNSPKPPIRPLPVYDVDGGPFAELVLRRSPDRELPRFELTFPEGITPSLPS
                     FESHSVSFA"
     gene            complement(4780..6159)
                     /locus_tag="PK710_14025"
     CDS             complement(4780..6159)
                     /locus_tag="PK710_14025"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOT10891.1"
                     /translation="MRVPGLVFASEAMMADIRKDASLDQVAHVAHLPGIVHHSLAMAD
                     IHWGYGFPIGGVAAFDEHDGVISPGGVGYDINCGVRLLRTRIPLQDVQPHLPALSSEL
                     LRAVPSGVGSKSHRTLSDQELETVLVQGAAWAVTSGFGRTADLDVTEERGTMRGANPG
                     TVSERAKERGLSQLGSLGSGNHFCEIGWVADVFDEQAANAFGLSQGCITVLLHSGSRG
                     LGYQICDDSLRTMLAASEKYQIELPDRQLCSAPLHSPEAIAYLHAMTCAVNYAFANRQ
                     MLTHFVRQSLVRFFAEPEPSLGLDLVYDGCHNVAKWERHNVDGKPRRLCVHRKGATRA
                     FPAGHPDVPKAYQHVGHPVLVPGDMGRYSYVVVGTQQALHDTWGSACHGAGRVLSRQA
                     ALKMSKKKKIFETLQAKGVWVRALSKRTVAEEIPEAYKDVADVVDVMTRAGLVRPVVK
                     LEPLAVIKG"
     gene            6349..6531
                     /locus_tag="PK710_14030"
     CDS             6349..6531
                     /locus_tag="PK710_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10892.1"
                     /translation="MTDGSWASGGRNSIVAGDAVLGRIGNRGKGEAIIEPWHAFVSGL
                     ILNRIVTLGIPTCAGV"
     gene            6578..>7634
                     /locus_tag="PK710_14035"
     CDS             6578..>7634
                     /locus_tag="PK710_14035"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOT10893.1"
                     /translation="MTPSMLALLAIIVVAFVLFIGGWVRADLVGMLVLSTLALTGLVT
                     PQQAVAGFGNPAVITVWAMFILSAAMTRTGVAHRLGKPLLRFAKSREPTIIVVLMIVS
                     SLLSALINTVTVAAILLPPTMELIRRSGRPASRLLMPLALGCLLGGPFTSISTTPNIL
                     ATEALKAAGLEPFGLFDFTGVTAAIVVVGTAFVAFWGRHLLPKRSPSAATDELGRSYD
                     LAAHLFSTQLPENSPLLGKTLAESHLASALHLTVVAIQRRGALLLAPRADEVLSLGDR
                     LIMHGRPDHLRRFHGSEHLRIESREELAGLEPLCGAIGQGVVHEGSPLVGLTMVQSDL
                     RREHGVHVLALGGCDGCI"
BASE COUNT         1635 a         1983 c         2377 g         1639 t
ORIGIN      
        1 aagagacaag atcgacgtaa gctccccgag acggaagcac ccattgatcg tctggttcgg
       61 gcaggttggt aacgatggtc gttggcccct cggcagacag ggcgagaacg cggtagtatt
      121 ctggttcggg aaccacggag atcaacgcgc cggctgctgc gacggctttg cttcggttgg
      181 ggctatgcgg cgcaatgaac gtcgtacccg cggttcgcag cggatcgagt tgttcttcga
      241 ggtggtcggc acagcaagaa cgatccgcga ggcgtttgaa atgcggagca tcggcagctt
      301 ctgccccgga gaaaacgacg atcggttgat ccgattcgat cagcgaacct gtgaaatccg
      361 acaagaaatc accggtttcc agattgagga cgtcgaaggg ttgcagcacg acctccaggg
      421 tatcggctgg tgcaaggggg cctatatttt ctccagccac cacatgcgtt gcggaggtga
      481 aacgaacgtg ggtatcgggg cgcgttccga tcatggtgac gaacgcttgg agttgaattg
      541 gcgacactgg attgaaattg gtatccggat cgtcggtgat ggcaatggtc tgaggccaac
      601 cgagcacgac gtatcccaat tccatcaagc cgctgccggg cagtgcttcc acgggcttga
      661 gcaaggaggc atcgttggaa aacacattga cgttatcgag tggattgaat tgatacgcga
      721 ccaccggcac atggctttgc acgcgatagg cttgtcgggt cagggccgtg ccgggtccgg
      781 tatcaaaaga gccgtgggca ctgccgtcca cttctcgtgg tccgagcaaa aacacgtgca
      841 atccgtaggg cggtatagag gcttccccca ccgttcgtgg ttgggaaggc gctcctacgg
      901 gcgcatcgtc gagttccaca acgacactgg caaacaggtc ggattgagga ttggaaacaa
      961 cgacagcaaa ctgttgtgcc gcggcgttgg agtctggact gacgttggca ttgtcgagat
     1021 cggcagccca gtattcacag cccacattgc tttttacgac agaagcttgg gaacaaagat
     1081 gcgtacacag tccatttcgg catgcgtatc cgtccgtggt gtcgcaaaaa cccgcggggt
     1141 cgaagcctga cccatcctga cgacaaacca gaacggtctg ttcatcgcag tctctggctg
     1201 aaggcaagca cgccacgcac gaaagcaacg acggggcgca caccagtcca cgagaggcgc
     1261 aatcgtcaat gatgttccag cgcgtgcgtc catcgatccg gtcacatcgc tgcagccctg
     1321 cctggcatcg catggtgccg ggttcgcagc ttggttgcgc gaggggaggc gtgtcatccc
     1381 agcggtcagc gcgatcgaag tgacactgaa ggttcgcaac acacagcaag atacccattg
     1441 ccaagggaaa agatttcgac cctgtggctg gagccatcat tctacacgcc catgcccctc
     1501 atcgcccttg cctccgaaca acgggaaatg gccgagcgaa cacgcgctaa ttggtgctac
     1561 cgtcgctcgt gactcgattg gcaagagact tcaagccttc ggaatccacg atgacgacat
     1621 cgaatttttc accgaaggcc gtcagcaatt tgttttggaa tacgaatcct tgaccgtgca
     1681 aaacgggcag ttcggtattc ggctcgaggc gtttgatatc gaagacatga aaaagcacgg
     1741 cgggaatgaa ttcattccaa agcgcgcgag cggtttcggg gtcttcaaat tggcaacgca
     1801 gacggtggtt tccgaattga tgcaaatgca gcttcatgcg tggctcgtga atgggttgaa
     1861 aagatgcttc gtcgatcgga gccagctttc gtcccggctc agaatctgcc gcatcacggg
     1921 caagacaagc agacaagccg gttgggtgca agcgacgatc aggctctgac ccccaggagc
     1981 atcaccgttc atcgggtcgt gaaggtgccg tccgtgggca gatccgcggg tgagccttcg
     2041 accgtatcga cgcgcgacag ccgcggtccg cgatgaagtt cgatttcgaa cgcttccaag
     2101 gcagcgggaa cgcccccggc atgaatggcc acggtgccgt cggggaggtt acgcgccgag
     2161 ccgatgatgc ccaaagatac ggccttttta cgcacgaatt cacggaaacc cacgcgctgc
     2221 actcgtcctc gaacgatata tttttttccg atcacgatat gagccccaag gtgtggttgg
     2281 tagcgcacag cccgggcgac gtccagaagg gaggcatcgc aaacggtgta agatcacggt
     2341 acgctcgatc gttaacggca aaaggacaac gccatgcctt catcgacccg actacgcgcc
     2401 cgttcgccca agccaagagc ccacgccacc actcccaact atcctcgcca aagcattctc
     2461 gttggggttg cgatgtctct cacggcttgt atgggagccg caccaggtcc atacgctcca
     2521 gctacgccgt cccagaccgt aacggtgaag tcggaggatt cgagatttgc gcagccgccc
     2581 cccgacgatg tcgtagaaga cactgcccag cccgttccgc ctccggatca cgatattcct
     2641 ccccccggag aggcccccgg tccattcgaa tgacagccgt atccgtcctc acccctggcc
     2701 ccacggaacg gagcatatta attcgaggcg cgcgatactg tgctccgtaa acatcatcgg
     2761 caacgtcccc tgcccttttc cctttccatc acgcctggtg ttccatcgtg ggtttcattt
     2821 cgcgggtcca tcacgaaggc ccgagatgct gaagcgacca atggtacatg gtgatcgcgg
     2881 cggccgctcc ggcgttgacg gaccgtgtgg aaccatattg gctgatggcc acgagctgtt
     2941 cacagccagc caccattcgt ggcgtcaggc caggcccctc ggaaccgaaa atcagacagc
     3001 aatctcgggg caagcgaaac gtctccaaag gaaccgcatt ttcgaaatta tccactccga
     3061 tgggtacgat ttgcctgtca cgcaaccatg aaaaaaactg cggttcatcc gcatgatgat
     3121 gaaccgtcaa gtaccggtcg gtgaccatgg caccgcgtcg gttccacttg cgacgcccca
     3181 cgatatgtac gccggcaaca ttgaaggcat tggcggtacg gacgatggaa ccgatgttga
     3241 aatcgtgttc ccagttttgg atggcgacgt gcaaagggtt tcgtcgagtg tcgagcacgg
     3301 cgacgatggc ctcgtttttc caataccgga agttgtccaa cacgttacgc cggtcgccat
     3361 tgcgtaagta ttccgggtcg agccgatcgt cttccggcca cggcaacgga tggggtccaa
     3421 ccccgaccgt gtgatggcac gggtattcga agtcactcac gccctcgtct ggatgaacgg
     3481 agctgtgaga agttgggtca tcgctcatgg aactactaat ttaactatgt ttttcaatat
     3541 gttatcgcat ttgcgaaggc tttagcttcc ggaaggaagc gccagagggt tggtagttca
     3601 ttggagattc gttcgtcaag gcggcgagga ttttctcaca gggcgagcgc atcgattatc
     3661 gatgcccatg gaaagaggtt tccgcagcta ggcagccacc taacagggca gcgcacgggt
     3721 caaggtctcc gacctgcggg gagtggctgt ctaacgacct ttttcagaag tcagaactcc
     3781 aaggcgcttg ccctgaattt tttccggctc gaccggaaag ccagttcaat tctcggggaa
     3841 aacgttgagc atgaaaaaaa acgtggtggc attgaaaaac atcacggagg ggcgaatgag
     3901 ttcatcgtcg gatccaacgg atcacgtctt gcaggaaggc cacggcaaac agaccaagtg
     3961 ccgatagcaa accgatgaga agggatcgtt cgaaagaaac gtggaaggtg gggagcatac
     4021 gtccgacgat gaagcccacg atgattggca agacataacg ccaggggatt tggatattgt
     4081 cgggtgttgc ggcaatggaa tatttgtcgt tgcgaccgaa agctttgcgt cgcgcgctat
     4141 ccacgtacct cgcgagagct tcgttttgcg cgtgaagtcg ttctggggca aggagttgtt
     4201 ctttgggctg tggctgttga cgaaacggtt ccatgagcgt tttcgcacgg ggcagatagc
     4261 tatggtagcc aatcatgttc ggggaagcat gtcgatgagc aagcgaggtc atgccgactc
     4321 cgaaggtggc acggcgacgg tcgtaaggca gatggatcga agaggcagga gcggatagct
     4381 atgcgaagga gacggaatgc gactcgaaag agggcaagct tggtgtaatt ccttctggaa
     4441 aggtgagttc gaagcgaggc aattcgcggt cggggcttcg tcgcaacacg agttcggcga
     4501 aaggaccgcc atcgacatca tacacgggca acggtctgat tggcggtttg gggctattga
     4561 cgatcaacac ggaaagaggg gagacacgaa agggttcgtc cacgaaacga atgacggtgt
     4621 ttggcccggg agccgctcgc gcgacgattt cgaagggcga tggggtggga ttcacccggg
     4681 gtcttgccaa gatacgtgca aacagcgatg ccaagggaac ctcaaaccag agttacgctc
     4741 aatgaatcat aggaaaacga ataattcagc tatatggggt taacctttga tgacggcaag
     4801 gggttcgagt ttcacgacag gtcggacaag tccggcgcgt gtcatgacgt caacgacatc
     4861 cgccacgtcc ttatacgctt ctgggatttc ttccgcaacg gttcgcttgg acaaagcgcg
     4921 cacccagact cctttggcct gaagagtttc gaaaatcttt ttctttttcg acatcttgag
     4981 agctgcctgg cgggaaagaa cccttccagc cccatggcag gcggaccccc aagtatcgtg
     5041 caaggcctgc tgggttccta ccaccacata actatatcgc cccatatcac cggggacgag
     5101 cacggggtga ccgacgtgct ggtacgcttt gggaacgtct ggatggcccg cggggaaggc
     5161 ccgggtggct ccttttcgat gcacacacag ccgccgtggt ttgccatcga cgttgtgtcg
     5221 ttcccatttt gcgacgttgt ggcagccgtc atacacaagg tccaacccca gggaaggttc
     5281 tggttcggcg aagaaacgaa cgagcgactg tcgaacgaag tgcgtgagca tctgacgatt
     5341 ggcgaaggcg taattgaccg cgcaggtcat agcgtggagg taggcgatgg cttctgggct
     5401 atgcagggga gcggagcaca gttggcggtc gggtaattcg atttggtatt tttcggaagc
     5461 ggcgagcatc gtacgcagcg agtcatcgca gatttggtaa ccgaggccgc ggcttccgga
     5521 atgcaggagc acggtgatac atccttggga gaggccgaat gcgtttgcgg cttgctcgtc
     5581 gaatacgtca gcgacccagc caatttcgca gaagtggttt ccggagccca aggagccgag
     5641 ttgagaaagg ccgcgttctt tggcgcgctc ggacacggtg ccggggtttg ccccacgcat
     5701 ggttcctcgt tcctcggtga catcgaggtc cgcggttctt ccgaatccgg acgtgacggc
     5761 ccaagcggcg ccttgaacca gcacggtttc gagttcttgg tcggaaagag tccggtggct
     5821 tttcgagccg acgccggaag gcacggcgcg caaaagttcc gaggaaagcg ccggaagatg
     5881 gggttgcacg tcctggagcg ggatcctggt tcgaaggagg cgcacgccgc agttgatatc
     5941 gtagccaact cctcctgggg aaatgactcc gtcgtgctca tcgaaagcgg cgacgccacc
     6001 gatggggaaa ccatatcccc aatggatatc ggccatggcc aaggagtggt ggacgatgcc
     6061 tggcagatgg gcgacatggg cgacctgatc gaggctcgcg tctttgcgga tatcagccat
     6121 catggcttcg gaggcgaata ccaggccggg cactcgcatc tttccttggc atggaatccg
     6181 aatacggacg tcgtcgatac gttgaacgcg gatagcagag cacatggggg tggtcagggc
     6241 gggagcttgg caccatggga aagcgtcacc aaggtcaatc ctacaattgc tgttgtcgta
     6301 cggcaatgtg agggcagcta taagatggcg cgagggacaa ggttgacagt gacggacggc
     6361 tcttgggcaa gcggcggtcg gaattccatc gtggcaggag acgcggtgct tggcaggata
     6421 ggaaaccgtg gtaagggcga agccatcatc gaaccatggc atgcatttgt atccggcttg
     6481 attctgaacc gaatcgtcac ccttggtatc ccgacgtgtg cgggggtgtg attcgtttgg
     6541 ctgaagctac ttctttcatc gtgtggggag actggtcgtg acgccatcga tgctcgcgct
     6601 gctggccatc atcgttgtgg cgtttgtgtt gttcattggg gggtgggttc gcgccgatct
     6661 cgtggggatg ttggtattgt cgacgcttgc gcttacgggg ctggtcacgc cgcagcaagc
     6721 ggttgcgggt tttgggaatc cggcggtcat tacggtatgg gcgatgttca tattgtcggc
     6781 ggcgatgacg cggacgggag ttgctcatcg tcttgggaaa ccgctgcttc gttttgcgaa
     6841 aagccgtgaa ccgacgatca tcgtcgtgct gatgattgtg tcgagcctgt tatcggccct
     6901 catcaatacg gtgacggttg cagctatttt gttgccgccg acgatggagt tgattcgtcg
     6961 tagcggtcgt cctgcttccc ggttgttgat gccgctagcg ttggggtgtt tgcttggggg
     7021 tcctttcacg agcatttcga cgacgccgaa cattttggct acggaggctc tcaaggcggc
     7081 ggggctcgag ccatttgggt tatttgattt tacgggggtc acggcggcga ttgtggtcgt
     7141 agggacggca tttgttgctt tttgggggcg tcatcttctt cccaaacgtt ccccgtctgc
     7201 agccaccgat gagcttggca ggtcgtatga cttggcagcg catctatttt cgacgcaact
     7261 acctgaaaat tcaccacttt tagggaagac gctcgcggaa agccacttgg cctccgcgtt
     7321 gcacttgacg gtcgttgcga tccaacgtcg gggggcgttg ctattggcac ctcgtgctga
     7381 tgaggttttg tcgttggggg atcgactgat catgcatggg cgtccggatc atctgcgacg
     7441 ttttcatggc agcgagcatc ttcggattga atcgcgagag gaattggcgg ggctggaacc
     7501 gttatgtgga gcgatcgggc aaggggttgt gcatgagggt tcaccgcttg tggggctcac
     7561 gatggtgcag agtgatttgc ggcgagagca tggtgttcat gtacttgcgt tgggggggtg
     7621 tgatggttgt atcg
//
LOCUS       DAOSOR010000257         7626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9609_length_7626_cov_3.437459, whole genome shotgun sequence.
ACCESSION   DAOSOR010000257 DAOSOR010000000
VERSION     DAOSOR010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7626
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9609_length_7626_cov_3.437459"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="PK710_14040"
     CDS             <1..932
                     /locus_tag="PK710_14040"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HOT10894.1"
                     /translation="KRAPAMADNRTLIRVLIAVFATFLVFASSDALAYCRTTTCDPSS
                     TNCDPPPGQACTTIGKELIWPNRCVGYSLQRDADPSVPFDTFARTTQRVFQSWLAADC
                     GQGKGPSIDVYDLGPIACDAVEYNRYAGNANIIMFRSSSWPYGLSQTLALTTVTFNTE
                     NGHIYDVDIEVNTAQVDVTTTDENVKYDLEAILAHEVGHFFGLAHSADKTATMYSKYS
                     QGSLDLRSPEPDDHEGLCAIYPPERKSQPCDPTPRHGLQQTCGDGQAPEEQGCSACAL
                     PAESMQQKRNWVWLVIVALGLGWRRASRRVSRG"
     gene            925..2088
                     /locus_tag="PK710_14045"
     CDS             925..2088
                     /locus_tag="PK710_14045"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HOT10895.1"
                     /translation="MVDSASVWWIDTADHLGAWLRQVHDVNRIAVDVESNGMFAYQPD
                     VCTIQFAWELPDGSTRAAIVDTLAIPLPALSSLLGDPFRLKVIHDLAFDARILNTRGI
                     VLRSVRDTSVAATYLRKPGSGLASLLAAEFGIQVSKEKQNSDWSQRPLSTESLAYLAD
                     DVYHLLRLDTRLDQQVQQAGIADEVATETAYRLMNALEESAPPSAPHLRIRGAERLGG
                     VQTRILEAIAAARETWAMEKNLPPQRLLADKLLLSMAQHPPRSMANLMRVGLVRKLTE
                     KQRTELLQAILFAEQDAKDHPEQFRKPVAPPMLPVAQRIANKNLEKRLQTWRSAEAER
                     RDVHEQVVLPTHCIRWLLSRKPTTEEQIASMPGLGKARAQRYAGRLLSLVTGE"
     gene            2085..3206
                     /locus_tag="PK710_14050"
     CDS             2085..3206
                     /locus_tag="PK710_14050"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10896.1"
                     /translation="MSNKNKHLLVCGAEPSSDRIASMTLSALSNAGANLQSFGVGGEA
                     SQASGMEVLEPLTGLAAMGWIPATRRAWALQRAFRVLVRECRRRRPGQALLIGFTSFN
                     QALGRELRSMGIRVLWCVAPQVWAWRPSRLDTLRHSVDRLAVLFPFEEPLWRRHGYDV
                     HYVGHPAVELTTYADSTSFPPRLAVMMGSRVQEVSASISPFLQAARRWLDQHPDGQAH
                     VIVSPALPSGWRRRCLAAVQSKGISYFLADRRDGAAVQLEAYSLALCVSGTACLEAAL
                     SGALPVIAYRCDPVSAFVVKKLLRTPHIGLPNILLESRVFPELVQGQMRADRLVAALE
                     QLRESDRARRACQQLRKLLTVGDGLGFGERMARLVQEGL"
     gene            complement(3266..4039)
                     /locus_tag="PK710_14055"
     CDS             complement(3266..4039)
                     /locus_tag="PK710_14055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT10897.1"
                     /translation="MLAFWPDVLEPILSALHPTSLVEIGSEAGKTTRLLLNWAKDNDA
                     RLIAIDPAPAFDIEAVQRTYEGIFQCFRLPSLVALSSVERFDVVLIDGDHNWYTVFHE
                     LQLIQTLCQQSGQPFPLVFMHDVAWPYGRRDLYYAPSTIPEPYRHPYARQGISPTCSR
                     LLPQGGFNARMYNALEEGGPRNGVLTAVEDFLAQSEASFEWVVIPAVFGLGILLPSWL
                     AQANPLVAQQIRVWATPEVQRFIQRLEMARIAMLTGVSA"
     gene            complement(4201..4989)
                     /locus_tag="PK710_14060"
     CDS             complement(4201..4989)
                     /locus_tag="PK710_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10898.1"
                     /translation="MSFRFNWALACAGFALALWACGSDDENAKEGQGGSSGSGGSGGQ
                     DPGKTVRLGATFLDYLSKNPIEGLEVCTLEPPGRDPGCAKTIATGQIVADGFPTHFEV
                     LFKLSKDDYLTTLIPAVTGEQDDTASVLYPVTASGAKLLFSTASLTMEPGKGAISFIA
                     DQSKPGESKEAGGQEGVGVTISPSSGTLAYLNGVTLDTNATATFASGLGVFVNVDPGE
                     YTLTFSHASKTCASRLGWSTAQSNVSKIPVKADMITYVLHQCGQ"
     gene            5158..6060
                     /locus_tag="PK710_14065"
     CDS             5158..6060
                     /locus_tag="PK710_14065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOT10899.1"
                     /translation="MTLAAPKSPEKLRTSLPRALPVGGPWLPPSHGPQVPFAKPFVKW
                     AGGKRKLVPLLLRHAPKTFGTYHEPFVGGGALFYALRPSRAVLTDANARLVRTYRGIR
                     DDVEGVIERLRSYPHDHDFFMKLRKTKVDEGSDADVAAWFVYLNKIGFNGLYRVNRNN
                     EFNVPFGDQPNPTICDEDNLRACSRTLRQTDIEVQDFSEVLTRARKGDLVYFDPPYLP
                     LSASSSFVAYTQGGFGPQDHERLRDVALMLKRRGVHVLLSNSAHPLVRELYGKHFHIE
                     EVLAARAINSRGDRRGPIRELVIH"
     gene            complement(6258..7487)
                     /locus_tag="PK710_14070"
     CDS             complement(6258..7487)
                     /locus_tag="PK710_14070"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOT10900.1"
                     /translation="MRNTVRLIPILLAVGALGACDETKTSSSKAAPSASFRVPPGQEA
                     PADVAAAPADAQKTAGGTAFKVLVKGTGDHKPTENDAVALEYTAWTPEGKPFGSSKTM
                     GRPARVGLAGVVPGLAEVLQDMTRGEERRIWIPAAQALKGWPHPPEATAITMDVKVID
                     IIAGPKKIPVPEDVAAPPADAKKTKTGLASKVLSEGTGKVHPKASDTVKVHYSGWTTD
                     GSMFDSSEARGQPASFPLNGVIAGWTEGVQLMVEGEKRRFWIPVELAYNNMPGKPAGM
                     LVFDIELLKIVRPPETPKDVAKPPTDAKKTESGLYSKVLVPGKGTKHPTATSRVKVEY
                     SGWTTDGKMFDSSITRGQPASFSLMSVIAGWTEGLQLMVEGEQRRLWIPEELAYKGQR
                     GKPKGMLVFDVELLEIQ"
BASE COUNT         1497 a         2098 c         2337 g         1694 t
ORIGIN      
        1 ggaagcgcgc tccggccatg gcggataatc gaacgttgat tcgtgtgctc atcgcggtgt
       61 tcgcgacgtt cttggtattc gcgtcttcgg atgctctcgc ttattgccgc accacaacct
      121 gtgatcccag ctcgaccaac tgtgatccgc cccctggaca ggcctgcacc accatcggca
      181 aggaactgat ttggccgaat cgatgtgtgg gctacagcct tcagcgggat gccgaccctt
      241 ctgttccgtt cgatacgttt gcgcgaacca cgcaacgagt attccaatcg tggctagccg
      301 cggattgcgg ccaaggcaaa ggtcccagca tcgatgtcta cgacctggga cccatcgctt
      361 gcgatgccgt cgaatacaat cgttacgcgg ggaatgccaa tatcatcatg ttccggtcgt
      421 cgtcatggcc ctatggcttg tcgcaaaccc ttgccctcac gaccgtgacc ttcaatacgg
      481 aaaacgggca tatttacgat gtggacatcg aggtcaacac ggcgcaggtc gatgtcacca
      541 ccacggacga gaatgtcaaa tacgatctgg aagcgatcct tgcgcatgag gtagggcatt
      601 ttttcgggct tgcgcattcg gcggacaaga cggcgacgat gtacagcaag tattctcaag
      661 ggtcgctcga tcttcgtagt ccggaacccg atgatcatga agggctttgc gcgatttatc
      721 ctcccgagcg aaaatcgcag ccttgcgatc cgacaccgcg tcatgggttg cagcaaacgt
      781 gcggcgatgg gcaggctccc gaggaacaag ggtgttcggc ctgcgcgctg ccggcggaat
      841 caatgcagca aaagagaaac tgggtctggc tcgtgatcgt ggccttagga ttgggctgga
      901 ggcgcgcgag tcgacgggtg tcgcgtggtt gatagcgctt ccgtgtggtg gatcgatact
      961 gccgaccacc ttggagcgtg gttgcggcaa gtgcatgacg taaaccgcat cgccgtcgat
     1021 gtggaaagca atgggatgtt cgcctatcag ccggacgtat gcaccatcca attcgcgtgg
     1081 gaattgccgg atggcagcac tcgtgcggcc atcgtcgata cgcttgccat cccccttccc
     1141 gcgttatcgt cgttactcgg tgatccattt cgactcaaag tcatccatga cctggccttc
     1201 gatgcaagaa ttctcaatac gcggggcatc gtgctgcgat ccgtgcgaga tacgtcggtg
     1261 gccgccacgt atttgagaaa gccgggctcg gggctcgctt cgctgctggc agcggagttc
     1321 ggcattcagg tgagcaagga gaagcaaaat tccgattggt cccaacgccc cttatcgacc
     1381 gaatctctcg cttatttggc ggatgatgtc tatcacttgt tgcgtctcga cacacggctg
     1441 gaccagcaag ttcagcaagc gggtatcgcc gacgaggtgg cgacggaaac cgcgtatcgc
     1501 ttgatgaacg cgctcgaaga gtcggcgccg ccctctgcgc cgcatcttcg cattcgtggg
     1561 gccgaaaggc tcggtggtgt ccaaacccgc attctcgagg ccatcgcagc cgcgcgggaa
     1621 acctgggcca tggagaaaaa tctgcctccc cagcgattgc tagctgataa attgctcctt
     1681 tccatggcgc agcatcctcc ccgttcgatg gcgaatctca tgcgcgtggg tctcgtgcga
     1741 aagctcacgg aaaagcagcg gaccgaactg ctgcaagcga tcctattcgc cgaacaagac
     1801 gccaaggacc atccggaaca gtttcgaaag cccgtcgccc cgccgatgct tcccgtcgcg
     1861 caacggatcg ccaacaaaaa cctcgaaaaa cgcttgcaaa cctggcgaag cgcagaagcc
     1921 gagcgtcgcg atgtccacga acaggtcgtc cttcccaccc attgtatccg gtggctgctg
     1981 agccgaaagc caacgacgga agagcaaatc gcaagcatgc cggggttggg aaaggcaagg
     2041 gcacaacggt atgctgggcg gctgttatcg ttggtaacag gggaatgagt aataaaaaca
     2101 agcacttact tgtttgtggc gcggagcctt cctcggatcg catcgcgtcg atgaccttga
     2161 gtgctttgtc gaacgctggc gccaacctgc agtctttcgg tgtaggggga gaggccagcc
     2221 aggcatcggg catggaggtg ctggaaccac tgacgggatt ggcggcgatg ggttggattc
     2281 cagcgacacg tcgcgcctgg gccctgcagc gggcgtttcg tgtgcttgtg cgggaatgtc
     2341 gtcggcgacg ccccggtcag gcgttgctca tcggatttac tagttttaat caggcgttgg
     2401 ggcgtgagct gcggtcgatg ggcattcgcg tgctctggtg tgtggctccg caggtgtggg
     2461 cgtggcgtcc ttcacgcctg gatacgttac ggcacagtgt cgatcgattg gcggtgcttt
     2521 tccctttcga agagcctttg tggcgccggc acggctacga tgttcattat gtgggtcatc
     2581 ccgcggtgga gttgacgacg tatgcggatt cgacgtcgtt tccgcctcgg ctggcggtga
     2641 tgatgggcag tcgggtgcag gaggtatccg cgagtatatc gccttttttg caggcggctc
     2701 ggcgctggtt ggatcaacac ccggacgggc aggcgcatgt catcgtatcg ccggcgctgc
     2761 cttcggggtg gcggcgacga tgcttggcag cagtacaatc gaaggggatt tcgtatttct
     2821 tggcggatcg gcgggatggg gcggcggtgc agttggaggc gtattccctg gcgctttgcg
     2881 tttcggggac cgcctgtctg gaggcggcgc tatcgggggc cctgcccgtg atcgcgtatc
     2941 gttgtgaccc tgtatccgct tttgtagtga aaaaacttct tcgtacgccg catatcggat
     3001 tgcccaacat cctgctggag tcccgagtat ttcctgaact cgtccaaggg cagatgcggg
     3061 ccgatcgttt ggttgcggct ttggagcagt tgcgagagag cgatcgggcg aggcgggctt
     3121 gtcagcaatt gcggaaactg cttaccgtgg gagatgggct tggttttggc gagcggatgg
     3181 ctcgtctcgt acaagagggg ctatgaacga acaaactgtg ggcgggccga agggcatgga
     3241 tgggttatga ttttggggtg agaatctagg ctgatacgcc cgtgagcatg gcaattcggg
     3301 ccatttcgag cctctggatg aaccgctgga cctcgggggt ggcccagacc cggatttgct
     3361 gcgctaccag gggattggct tgcgccagcc aggagggcaa caagattcca aggccaaaaa
     3421 ccgccggaat cacaacccat tcgaaggatg cttccgactg ggccaaaaag tcctccacgg
     3481 ccgtgagaac accgttgcgc ggcccccctt cctccaacgc gttatacatg cgcgcattga
     3541 accctccctg gggcaacaac cggctgcagg tgggggagat gccctgccgc gcatagggat
     3601 gccgatacgg ttccggaatg gtgctcggtg cgtagtacag gtcccgacga ccatagggcc
     3661 aggccacatc atgcataaag accaggggaa acggttgccc cgattgctga cagagcgttt
     3721 gtatcaactg aagttcgtga aataccgtgt accaattgtg gtctccatcg atcagcacga
     3781 cgtcgaagcg ctccacggaa gagagcgcca caaggctagg taagcgaaaa cattgaaaaa
     3841 ttccttcata cgtccgttgg acggcctcga tgtcgaacgc gggtgccgga tcgatggcga
     3901 tgagccgggc gtcgttatct tttgcccagt tgagcaataa tcgggtagtt tttccggctt
     3961 ccgagccgat ttccacgaga ctcgtcgggt gaagggccga aaggatcggt tcgaggacgt
     4021 cgggccaaaa tgcaagcatg gggggattat cggcacggcc gcggtccgac ggcaagggga
     4081 agcatgacaa ccttggtggc gaggcggcgg cagggtatag cggcattccc tccgcgattc
     4141 accatatgcc gaccgatgga aacagggatg ggtggctggg ttgggagcga tccgtcgggt
     4201 tcattgcccg cattggtgca ggacataggt gatcatatca gcttttacgg gaatcttgct
     4261 gacgttggat tgagccgtcg accatcccag ccgcgaggcg caggttttcg aagcatggga
     4321 gaacgtaagg gtgtattcgc cggggtccac attgacgaaa acgccgaggc cggaggcgaa
     4381 ggtggcggta gcgttggtat ctaaggtgac gccgttgaga taggccaggg ttccgctcga
     4441 cggtgagatg gtgacgccga cgccttcttg tcctccggct tccttggatt cgccgggttt
     4501 tgattggtcg gcgatgaaag aaatggcgcc tttgcctggc tccatggtca aggaggcggt
     4561 cgaaaacagc agtttggcgc cggaagccgt gacggggtag agaacggaag cagtatcgtc
     4621 ttgctctccg gtcacggcgg ggatgagggt ggtcaagtag tcgtctttgc tgagtttgaa
     4681 gaggacttcg aagtgggtgg ggaatccatc ggctacgatt tgtcctgtgg cgatggtttt
     4741 cgcgcagccc gggtctcggc ccgggggttc gagggtgcag acttcgaggc cttcgattgg
     4801 atttttggat agataatcca aaaacgtggc gccgagtcga acggttttgc ctggatcctg
     4861 gccgccggaa ccgccggaac cgctgctgcc gccctggccc tcttttgcgt tttcgtcatc
     4921 gcttccacac gcccacaagg ccaacgcaaa ccccgcacaa gccaaagccc aattgaatcg
     4981 aaatgacatg attgtctcct gatatactgt ccagggggaa gatatcctag cccagacgct
     5041 ccgatatcac gcttgaggga ggcaaacaag caagcctttc gcaatggatg aaaacatccg
     5101 ataacttggc tgtatcggcc ttcctggggc atgtcacggg gtggaaagga tcggcggatg
     5161 accctcgctg ccccgaaatc acctgaaaaa ctaaggactt ccctgcccag ggccttgccc
     5221 gtcggtggcc cttggctgcc cccttctcat ggtcctcagg ttcccttcgc gaaacctttc
     5281 gtcaaatggg ctggtggaaa gcgaaagctc gtcccgctgc tgcttcgcca cgctcccaaa
     5341 acgttcggca cctaccacga acctttcgtg gggggaggag ccctttttta cgccctccga
     5401 ccttcccgcg cggttctcac cgatgccaac gcccgtctcg tacgcaccta tcgcggcatt
     5461 cgtgatgacg tggaaggagt catcgaacgg ttgcgaagct atccccatga ccatgacttt
     5521 tttatgaaac tacgaaaaac caaggtggat gagggcagcg acgcggacgt ggccgcgtgg
     5581 tttgtctact tgaacaaaat aggcttcaat ggcctgtatc gtgtcaaccg caacaatgaa
     5641 ttcaacgtgc cttttggtga tcagcccaac cctaccatct gcgacgaaga caatctgcgc
     5701 gcttgctcac gcacccttcg gcaaaccgac atcgaagtgc aggacttttc cgaggttttg
     5761 acccgcgctc gaaaaggaga ccttgtttac ttcgatccac cgtatctacc gctcagcgca
     5821 agctcttcgt tcgtggcata tacccagggg ggattcggcc cccaagacca cgaacgactc
     5881 cgggatgtcg ctcttatgct caagcgccgc ggcgtgcatg ttctgctttc taattccgcc
     5941 cacccgctcg tgcgagaact gtacggaaaa catttccaca tcgaagaagt cctggcagcc
     6001 cgggccatca atagccgcgg agatcgtcga ggacccatcc gcgaattggt catccattga
     6061 ccccgtttct cgttcgtttg gtataaaatt caataaatat aaatagttgc catgacttcc
     6121 gcgattcggc acaaggtccg tgggcgcttc ctttgaggcg tgatggtttg cgacaaaggg
     6181 aaggtggaga aagggggaat tttacgctcg agccctgggt atcctacgag gggaacacag
     6241 ggtccgttgc gcgtaagcta ttgaatttca agcaattcta cgtcgaatac gagcattccc
     6301 ttgggctttc ctcgctgccc tttataggcc agttcttcgg gaatccatag ccggcgttgt
     6361 tcgccttcga ccatgagctg gagtccttcg gtccatccgg cgatcacgga catgagagaa
     6421 aacgatgcgg gctgcccccg tgtgatggag ctatcgaaca ttttgccatc ggtcgtccag
     6481 cccgagtatt cgactttgac acggctggtg gccgtgggat gcttggttcc ttttccgggg
     6541 acgagcactt tcgaatacag gccgctttcg gttttttttg cgtcggtggg gggtttggcc
     6601 acgtctttgg gtgtttctgg gggacggacg attttgagca gctcgatgtc gaatacgagc
     6661 attccggcgg gttttccggg catattgtta tacgccagtt ccacgggtat ccagaatcgt
     6721 cgtttttcgc cctcgaccat gagttgcacg ccttcggtcc acccggcgat gacgccgttc
     6781 aaggggaagg aagcgggttg tcctcgggct tcggaagaat cgaacatgct gccatcggtg
     6841 gtccagccgc tataatgcac tttgaccgta tcgcttgcct tggggtggac ctttcccgtg
     6901 ccttcggaca agaccttgga tgcgagtccg gttttggttt ttttcgcatc ggcgggggga
     6961 gcggctacat cttcgggaac cggaattttc ttgggtccgg cgatgatgtc gatcaccttg
     7021 acatccatcg tgatcgcggt tgcctccggc ggatggggcc atcctttgag cgcctgggca
     7081 gcgggaatcc agatgcgtcg ttcttctccc cgggtcatgt cttgcagcac ttccgccagg
     7141 ccgggcacga cacctgccaa accgacccgc gcgggtcttc ccatcgtttt ggagcttccg
     7201 aagggttttc cctcgggagt ccaggcggta tattcgagtg cgaccgcatc attttccgtg
     7261 ggtttgtggt cgcccgtgcc tttgacgagc actttgaatg ccgtgccgcc ggccgttttt
     7321 tgcgcgtcgg cgggagcagc ggccacatcg gcaggggctt cttgccccgg aggaacccgg
     7381 aacgaagcgg agggggccgc cttcgaagaa ctcgttttgg tttcatcgca agcgccgagc
     7441 gctccgacag ccaagagaat agggatgagt cgtaccgtgt tgcgcatggc cgggcactcg
     7501 aacacgttcc gtgccgttcg tcaaagggga tagtccatcc taccgcaaaa aatcttctcc
     7561 cgcggtcgtc aatccacccg tgggtcccca ttgcaatctc cccacccacg tcgtcttgcg
     7621 gtaccg
//
LOCUS       DAOSOR010000258         7616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9628_length_7616_cov_4.612088, whole genome shotgun sequence.
ACCESSION   DAOSOR010000258 DAOSOR010000000
VERSION     DAOSOR010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7616
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9628_length_7616_cov_4.612088"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PK710_14075"
     CDS             <1..231
                     /locus_tag="PK710_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10901.1"
                     /translation="HQVLIEGDAACHPEPEQGEQRYEMGGVALGYNVEVSDALELISQ
                     LYFDVPQAHESVSTGVMLGLIGTLPSPLATNP"
     gene            338..3613
                     /locus_tag="PK710_14080"
     CDS             338..3613
                     /locus_tag="PK710_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10902.1"
                     /translation="MRVSFRRILAVSMAAVVAGVLAACGSASSKPSIVTSPVPAVSAL
                     PAWSYHPASPQRLLSRLDLEDAHRLFVGAGGERWLVDLRAAVATAAPILADQDLIAVS
                     RLENGSFMFVGKEGSIFTSPSPLGSLTLAHRLEPIPMRIVAAGQRIIALHWDGSAVQS
                     FDGGKTFGKIELGKGHPYDVALGPEGHAMILGFPEKLWLSKGQGDWTVAPTPPVGAGF
                     VGLDARGALVAQGIRTSVVWDGLSEVPVQQARRFDPPALDLLVDLEVGPSATALLRGS
                     ATITDGQYVEVARREEKGLWHLGRGKLTERLTWVPIPDTDVCRHLFVDAKGHKLVLAC
                     LMGARKGVRFPYLRLFRSDDGGNTFSWQPTTLVGDEEGVSLTPIGDALILAGVCKPDS
                     DGVCVPGPPVRLTGDETSKKYVNSISSIIPPLLGRVTRVVAIADRLFAVGLAKTGEPV
                     VLVSEDQGKTFRSRSIDLSTWVDGQASLSAMHQGKLVVGERQEMSWVFGRKKGDVWVV
                     FDATGRVMSARLVPADHKLIAAGSRALAIHVDDGTVLESLNGGETYEHLTRFPSSMTK
                     ESLDAWCEKDGCVIGETFSRAGWRGKEGGVVESQERATPSKEPSYRTTISCRVVDDVT
                     GVVPNAIHLPNATSVHRGATAWSVVFSDSKTASAGVAHATSDRGQRVTPVLLLPPKPN
                     AAGVAVHARTQVEGAAALRYTFATDADGSARIGSPMRVEVAWDNQLEGSVYRTTLPTP
                     EPLRAGDVTIANDVATANAAILSITSDGIHVCPHAQCDPNSEPSFFVHRRGAVEVVPP
                     IPWPSEGLGGALALNHELIRLGGKNVSVALLQHRPVVLRSRERRDGSYQFDALALAPL
                     KAKMEGIDDFMIWSYLSTSTMGLSYLLYKPEAGLVRAYVLRFDTSEGVAEVLEAPTPK
                     NLSDPPVSCTEAQRRDSFRIVSPRISGTEHRVVVQHGSHRYVMTTDLSVLYGSPSSAC
                     VDALDARSDRRPEFRALISMRDMQHSWLLAKQGTTLRWWGLACLFDPDAVLKESEETP
                     IPVPITDEKDCAEVFEKLSRLIGHDNFPREILLQLCQQQKIDIPCVRSITDASQMARC
                     IRP"
     gene            complement(3643..4848)
                     /locus_tag="PK710_14085"
     CDS             complement(3643..4848)
                     /locus_tag="PK710_14085"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOT10903.1"
                     /translation="MEGMIGADRALKLVLQHVPQPRSELCSSERALGRYLAETIVATH
                     DVPSFCRAKMDGFAVRVHDAGKEIHVRDVVGAGHTTARIVESGHGIQIMTGAPCPTGT
                     EAIVMIEDIVRVEDRIRLPENIILHQHMEAVGELCQQGNILVQPPARITPWLIGVLAA
                     FDRGAIHVFRLPRMALISTGDELATAGEALRPGQIRDSNGIMLRSLAQEQGVDDTTLL
                     HASDSHKSLLSAMNSAEHADIVVLSGGVSMGLFDLVAEVLEHLGATIVFRKVQQTPGK
                     PMLFAVAGEKLFFGLPGKPQSTHFCFSRYVAPALRAFAGDPLPVWEGVGILAADSPCE
                     GNGTRFIPMQAQFHPQHFVALYPFDKAQIRGFFGSSQANAYVRVEPHVHRLRKGEPMP
                     FLWVSRMPE"
     gene            complement(4877..6130)
                     /locus_tag="PK710_14090"
     CDS             complement(4877..6130)
                     /locus_tag="PK710_14090"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HOT10904.1"
                     /translation="MDASEIEVALEELEQRVERLRALFEQYFMGIEKIEPSVLRKDVE
                     RRFWILRRTQIRNTAMRFKLNTISQRYNTYQQYWQRSMREIEAGTYRRHLVRAEKRFG
                     QEALTIAARKRLGKAGADAADADKTERDTRAQKAIEQVPDLDSFDVDMDFQDMLETTA
                     PNTADAHPLNELDDLDSLMGLSTAPSYHEPPRPPPPPRPASQRQSPSSFHTTQKQDEF
                     GDIGGIFSEDDDEQTAPHVRLPWPSNPAPEPTDRVASVPMGSKPVAGAVPRPLGPPAP
                     PARPTIPSASAAPVRTRPAAPPHSPAPPIPAAAPVRPPPAPPSAPSRPAVRAAPPTRA
                     SQQPSTPTSTSNLTDERVREIYREYVNAKRQCNESTSNVTEASLAASLRSSETKLSEK
                     HKGRRIDYEVVIKDGRAVLKPVVKS"
     gene            complement(6171..6962)
                     /locus_tag="PK710_14095"
     CDS             complement(6171..6962)
                     /locus_tag="PK710_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10905.1"
                     /translation="MPRITPRFARVLLTIAVGITACVPSQPSDSKPDAAPKTTKKAES
                     KPRTAPTHSAGRRSTPPNEQPPDPLLTAPFEDRFDRTDIGSDWRSLGNSWSIKNGKLC
                     VRQARNQGIWLRRRLPINARIEFEAVSESPDGDIKAEYWGDGLSGATSVSYTNATSYL
                     TIFGGWKNSFHVLARIDEHAPNRLEVRTNLTSPQEREKPVTPGRSYTFRVERTDGKTI
                     SWWVDGHLIHRLEDPEPLVGRGHDHFGFNNWEVPICFDNLMITPL"
     gene            7068..7403
                     /locus_tag="PK710_14100"
     CDS             7068..7403
                     /locus_tag="PK710_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10906.1"
                     /translation="MSDERKPSEDFKDGLELIFRAARSAVKQVDVSKIDKSLDKAISQ
                     ATRVATTVGRVVADELNRMSNKPPPWQKQSGDTSENAPAAGDEGRVWASEEQQVDPEK
                     AENEADEES"
BASE COUNT         1522 a         1987 c         2346 g         1761 t
ORIGIN      
        1 catcaagtgt tgatcgaagg ggatgccgct tgtcatcctg aaccggaaca gggggagcag
       61 cgatacgaaa tgggcggggt ggcgttgggg tataacgtcg aggtatccga tgccctcgaa
      121 ctcatctctc aactgtactt cgatgttccc caggcgcacg agagtgtgtc aacgggtgtg
      181 atgctgggtt tgattgggac gcttccctcg ccattggcga caaatccgta agaatcctgg
      241 atcgtcggcc ttagttctag cgaccaggca ttcactgttt ccgtggaccc aaagaagtta
      301 cgccgtgtac gtgccgagcc gtgtacgatg acgcgtcgtg cgtgtctctt ttcgtcgaat
      361 cctcgcggta tcgatggctg ctgtcgtcgc tggtgtcttg gccgcctgtg gtagtgcatc
      421 ctcgaaacct tcgatcgtga caagtcctgt cccggccgtg tcggccttgc ccgcctggtc
      481 ctatcatccc gcctctccgc aacggcttct ttcacgtttg gatctcgaag acgcccatcg
      541 cttgtttgtc ggggctggcg gagaacggtg gttggtcgat ttgcgtgcgg cggtagccac
      601 ggcagcaccg atactcgcgg atcaagatct cattgccgta agccgtcttg agaatggctc
      661 gttcatgttc gtgggaaagg aaggctctat cttcacttcg ccttccccct tggggtcgct
      721 gaccctggcg catcgtctcg aacctatccc gatgcggatt gttgcggcag gccagcgtat
      781 catcgcattg cactgggatg gcagtgccgt gcaatcgttc gacggtggga aaacgtttgg
      841 caagatcgag ttgggtaaag gtcatcccta cgacgttgcc cttggtcccg aggggcacgc
      901 catgatcctc gggttccccg agaagttatg gttgtcgaaa gggcaagggg attggacggt
      961 cgctcctaca cctccggtgg gtgctggttt cgtcggattg gatgcacgcg gtgccctggt
     1021 ggcacaaggg attcgcacct ccgtggtgtg ggacggcctc tccgaggtgc ctgtccaaca
     1081 ggctcgtcga ttcgacccgc ccgccctgga tctgctcgta gacctcgaag tggggccgag
     1141 tgcgacggct ctactccgag gcagcgccac gatcaccgat gggcagtacg tggaggtggc
     1201 gaggcgagaa gaaaaagggc tgtggcatct cggacgcgga aaactcaccg aacgcctcac
     1261 gtgggtgccc attcccgata ccgacgtatg tcggcatctg tttgtcgacg ccaaaggcca
     1321 caaactcgtg ttggcgtgcc tgatgggtgc gcgcaaaggt gtccgattcc cctacttgcg
     1381 gcttttccgc agcgatgatg gcggtaacac gttttcttgg cagcccacga ccctcgtagg
     1441 tgatgaagaa ggcgtatcgt tgaccccgat cggggatgct ttgatccttg ccggagtatg
     1501 caaaccggat tcggacggag tctgtgttcc gggccccccc gtgcggctca ccggggacga
     1561 aacctcaaaa aaatatgtaa attcaataag ttccatcatt ccgccgctgc tcggtcgcgt
     1621 cacgagggtc gtggcgatcg cggaccgact cttcgccgtc gggctggcga aaaccggaga
     1681 gcccgtcgtt ctcgtatccg aggatcaagg aaaaaccttt cgttcgcggt cgatcgatct
     1741 ttcgacatgg gtcgacggtc aggcatcgtt gtccgcgatg caccagggca agcttgtcgt
     1801 gggggaacgg caagagatgt cgtgggtatt tggaaggaaa aagggagatg tctgggtggt
     1861 gttcgatgcc actggtcgtg tcatgtcagc gcgattggtg cctgcggatc ataagttgat
     1921 tgccgcgggc tcacgggctc ttgcgattca tgtcgatgac gggaccgtgt tggagagttt
     1981 gaacggtggt gaaacgtatg agcacctgac gcgatttccc tcgtcgatga cgaaagaaag
     2041 cctggatgcc tggtgtgaga aagacggttg tgtcattggc gaaacgtttt ctcgcgctgg
     2101 ttggagaggg aaagagggtg gggtggttga gtcgcaagag cgcgcgacgc cttcgaaaga
     2161 gccatcttat cgaaccacga tttcctgtcg cgttgtcgac gatgtgacgg gagtcgtgcc
     2221 gaatgccatt caccttccta acgcgacgag cgtccaccgg ggagcaaccg catggtcggt
     2281 ggttttcagt gattcgaaaa cggcgtcggc gggtgtcgct catgcgacaa gcgatcgggg
     2341 tcaacgcgtg acgcctgtcc tgttgttgcc tccgaaaccc aatgctgcag gggtggcggt
     2401 gcatgcgcga acgcaagtcg aaggagctgc tgctttacgt tatacgttcg cgaccgatgc
     2461 cgacggatcc gcgcgaattg gaagtcccat gcgcgtggag gtggcatggg acaaccagtt
     2521 ggaaggatcc gtgtaccgaa ccaccttgcc aaccccggag ccattgcgtg caggagacgt
     2581 cacgatcgcc aatgatgtag ccactgcaaa tgctgccatc ctgtcgatta cttccgacgg
     2641 tattcatgtc tgtccccacg ctcaatgcga tccaaactcg gaaccatcgt ttttcgtcca
     2701 tcgccgagga gcggtggagg tagtgcctcc cattccttgg ccgtccgaag ggctgggtgg
     2761 tgctttggct ttgaatcacg aactgattcg ccttggcggg aagaacgtat cggttgcctt
     2821 gcttcagcac cgccccgtgg tgcttcgttc cagggagcga agggatggct cgtatcagtt
     2881 cgatgcgttg gcgttggcgc ctttgaaagc caaaatggag ggaatcgatg attttatgat
     2941 ttggtcgtac ttgtccacct cgaccatggg attgtcgtac ctgctgtata agcccgaagc
     3001 gggattggtc cgtgcctacg tgttgcgttt cgacacgagc gaaggcgtgg cagaggttct
     3061 ggaagctccc acgccaaaga atctttcgga cccccctgtg tcttgcacgg aagctcaacg
     3121 acgtgactcg tttcgtatcg ttagcccgcg gatctcgggt acggaacacc gcgttgtcgt
     3181 gcagcacggt tcgcaccggt atgtcatgac gacggatcta tccgtgcttt atggaagtcc
     3241 atcctcggct tgtgtggatg cgttggatgc gagaagcgac cgacggcccg aattccgtgc
     3301 tttgatctcg atgcgcgaca tgcagcattc ctggttgttg gccaagcagg gaaccacgtt
     3361 gcgttggtgg gggcttgcct gcctattcga tccggatgcg gtgttaaaag aatcggaaga
     3421 gacgccaatt cctgttccga tcactgacga aaaggattgc gccgaggttt ttgaaaagct
     3481 gtcccggcta ataggtcacg acaatttccc gcgcgagatc cttcttcagt tatgccaaca
     3541 acaaaaaatc gacattccct gcgttcgttc catcaccgat gcttcccaaa tggcgaggtg
     3601 tattcggcca tagaacacca agacattctt tcggtgattg gatcactctg gcattcggct
     3661 tacccacaag aagggcatgg gttcgccttt tcgtaaccga tggacgtgcg gttcgacgcg
     3721 cacgtaggcg ttggcttgtg acgagccgaa aaacccgcgg atttgtgctt tatcgaaggg
     3781 atagagagcc acaaagtgtt gggggtgaaa ctgcgcctgc attgggatga aacgtgtacc
     3841 gttgccttcg caagggctat cggcagcaag aattccgaca ccttcccaca cgggcaaagg
     3901 gtcgccagcg aaggctcgta gggcaggagc gacatagcgt gaaaaacaaa agtgtgtgct
     3961 ttgcggtttt cctggaaggc cgaagaacaa tttttcgccc gcaactgcaa ataacatagg
     4021 ttttccaggt gtttgctgca ctttgcggaa tacgatggtg gcgccaaggt gttcgaggac
     4081 ttcggcaacg agatcgaata ggcccatcga cacgccgccg gaaagcacga cgatatcggc
     4141 atgctcagcc gagttcatcg ccgaaagcag ggacttgtgc gaatcgctgg cgtgcaagag
     4201 cgtggtatca tccacgcctt gctcctgcgc gagactgcgc aacatgattc cgttggagtc
     4261 tcgaatctga cccggccgca aggcctcacc agccgtcgcg agttcatccc ccgtggaaat
     4321 aagggccatt cgaggtaagc gaaatacgtg gatcgcaccg cgatcgaaag cagccagcac
     4381 gccgatgagc caaggcgtaa tccgtgccgg aggctgtacg aggatgtttc cttgttggca
     4441 cagctccccc acggcctcca tgtgttggtg aaggataatg ttttcgggca accgaatacg
     4501 atcctcgaca cgcacgatgt cttcgatcat gacgatcgcc tcggtgcccg tcgggcaggg
     4561 ggcgcccgtc atgatttgta tcccatggcc tgattccacg attcgtgcgg tggtgtgccc
     4621 ggctcccaca acgtctcgaa cgtggatttc ttttcctgca tcgtggacac gaacggcaaa
     4681 gccatccatt ttcgcgcggc aaaacgaggg aacgtcgtga gttgccacga tggtttccgc
     4741 gagataacga ccgagcgctc gctccgagga gcaaagctcg gatcgcggtt gtggcacgtg
     4801 ttgaagaacg agcttaaggg ctcgatcggc accgatcatg ccttccatgg attcgattcc
     4861 tccatgaaca ctgcacctag cttttcacaa cgggtttgag taccgcccgg ccatccttga
     4921 tgacgacttc gtagtcgatc cgacgtcctt tgtgcttttc gctcaatttc gtttccgaac
     4981 ttcgaaggct tgctgccaga gacgcttcgg tgacgttgga cgtcgattcg ttgcactgcc
     5041 gttttgcgtt gacgtattcg cgatagatct cgcgaacgcg ttcatcggtc agatttgacg
     5101 tggaagttgg cgtgcttggc tgctgcgaag cgcgcgtcgg aggagcagct cgtacggcag
     5161 gtcgtgaagg cgcggaaggt ggtgctggag gtggtcgaac cggagcggct gccggtatgg
     5221 gaggcgcagg tgaatggggg ggcgcggcgg gtcgagttcg aacaggagcc gcagatgcgg
     5281 aaggaattgt cgggcgcgca ggaggcgcgg gagggcccaa cggtcgaggt actgctcctg
     5341 ctacgggctt tgaccccatt ggcaccgaag caaccctatc ggtgggttcc ggagccggat
     5401 ttgacggcca cggcaatcga acatgaggcg cggtttgttc gtcgtcgtct tctgagaaga
     5461 tgccgccaat atctccgaat tcgtcctgtt tttgggtggt gtgaaacgac gatggagatt
     5521 ggcgttggga ggctgggcga gggggtggcg gtggacgcgg cggctcatga taggacggag
     5581 ccgtgctcaa tcccatcagc gagtcgagat catcgagttc attcaacgga tgagcatctg
     5641 cggtattcgg tgccgtcgtt tcgagcatgt cttggaagtc catgtcgacg tcgaaagaat
     5701 ccaggtcggg gacttgttcg atggcttttt gggcgcgagt atctcgttcg gttttgtcgg
     5761 cgtctgctgc gtcggctcca gcttttccaa gacgttttcg ggcggcgatg gttagggcct
     5821 cttgtccaaa tcgtttttcg gcgcgcacga gatgtcttcg atacgtgccg gcttcgatct
     5881 cgcgcatact tctctgccag tattgctgat aggtgttgta gcgctgactg atcgtattga
     5941 gcttgaagcg catcgccgta tttcggattt gtgttcggcg caaaatccag aagcgtcttt
     6001 cgacgtcctt gcgcaatacg gacggctcga ttttttcgat gcccatgaag tattgctcga
     6061 acaaggcacg gagacgctcg acgcgttgtt cgagttcttc gagcgcgact tctatctcgc
     6121 tcgcgtccac ggttatgaat ccttccctcg ttgcagcatg acgcaatgcc tcacaggggg
     6181 gtaatcatga ggttgtcgaa acagattggg acttcccagt tgttgaatcc gaagtgatca
     6241 tgcccacggc caacgagtgg ttcgggatct tcgaggcggt gaatcagatg cccgtctacc
     6301 caccaagata tcgttttccc atccgtgcgt tcgacccgaa atgtgtagga acgtccaggg
     6361 gttacaggtt tctcgcgctc ttggggggag gtcagattgg tccggacttc gagccggtta
     6421 ggagcgtgtt catcgattcg ggcgaggacg tgaaacgaat ttttccatcc accgaaaatg
     6481 gtcaggtagc ttgtggcatt cgtatacgaa accgaagtcg ctccgcttaa accgtcgccc
     6541 cagtactccg ctttgatatc tccgtctggg ctttccgaaa cagcctcgaa ttcgattcga
     6601 gcgttgatgg gaagacggcg tcgcaaccag attccttggt ttcgcgcctg gcgaacacat
     6661 agttttccgt ttttgatgct ccaagaattg cccaaggaac gccaatccga accgatatcc
     6721 gtgcggtcga agcggtcctc gaagggtgcg gtaagaagtg ggtcgggcgg ctgctcgtta
     6781 ggtggggtcg agcgccggcc cgcggaatgg gtaggggctg ttcgcggctt ggattcggcc
     6841 ttttttgttg ttttgggggc cgcatccggc ttggaatccg agggttggct gggaacacaa
     6901 gccgtgattc ccacggctat cgtgagcaaa acccgggcaa atcgaggggt aatcctaggc
     6961 acgccaagca tcctagcaca cccttttcgt agggaaggaa cgtcctgttg tcatgacacg
     7021 tcattgaggg ttgcgtttgc aggtcgagct ggccattgtg ttggttcatg agtgacgagc
     7081 gcaaaccatc cgaagatttc aaagacggtc tagagttaat ttttcgcgcc gcgcgttcgg
     7141 cggtgaaaca agtggatgta tcgaagatcg acaagagcct cgacaaggcg atctcccaag
     7201 ctaccagggt agcaaccacg gttgggcgtg tcgtggccga tgaactgaac cggatgtcga
     7261 acaagcctcc cccttggcaa aaacaatccg gtgacacgtc agaaaacgcg ccagccgcgg
     7321 gggatgaagg acgtgtatgg gcaagcgaag aacagcaggt cgacccggaa aaagccgaaa
     7381 acgaagctga cgaagaatca taagtccctt gatccgaaca aggggatgaa cgatgagcgg
     7441 ctggctatca tctagccagc gcatcacgtt tcgtctgcac ttgtccccga tttggattct
     7501 tctttcaagc gtttggcttc ggccaatcgt tctcggatga gttccttgac tttgggtaga
     7561 ttgtaaaacg ctgggcgaag accgtcggac gactgagcta cctcccaagc gacatg
//
LOCUS       DAOSOR010000259         7606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9649_length_7606_cov_4.151238, whole genome shotgun sequence.
ACCESSION   DAOSOR010000259 DAOSOR010000000
VERSION     DAOSOR010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7606
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9649_length_7606_cov_4.151238"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(350..640)
                     /gene="cas2"
                     /locus_tag="PK710_14105"
     CDS             complement(350..640)
                     /gene="cas2"
                     /locus_tag="PK710_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOT10907.1"
                     /translation="MRQTYVVAYDISDPKRLRKVYKTMRGYGEHVQLSVFRCELTEME
                     RLELRGKLGEIIHHREDQVLFVDVGPAHGRAMTSFSSIGRPYGQAPLLAIVV"
     gene            complement(651..2516)
                     /gene="cas1"
                     /locus_tag="PK710_14110"
     CDS             complement(651..2516)
                     /gene="cas1"
                     /locus_tag="PK710_14110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HOT10908.1"
                     /translation="MSNKVSSAQSPCPAKQPRAKTLPWEPQLPSSAPELVPARMINEA
                     LYCERLMYLEWVQGEFSHNTFTVEGKLVHRRADEPAGRLPPRPDESIEDSPASDDDIP
                     YAARSVWLSSEKLGITAKIDVVQGNESGEVIPIEYKRGKAPEYDWIRNPERAQLAAQA
                     MLLREHGYICNEAALYFAGSRRRVSIALEPSLLAMVVSTVRRIKELTAKGQIPPPTKD
                     EQKCQGCSLVGICLPDETRLLHDRPEEGEPASLAQQLSLYPELTGPLEPDPWQLAGTG
                     EAALTRRLHPARDDKMPLYIQEQGAQIRVEGELIIVRRQAGAAIEVRLPMLSQVMVLG
                     NVQITTQAIRALMERDIPLTYFTYGGWMYSRVRGLSSKNVELRVAQVRAAMDPQFCLK
                     VARGLVVGKVKNSRTMLRRNAIGVTAQALSELDQLAYKAERAEALESLLGLEGTAART
                     YFGEFTRMLKSKVEPAFELDGRNRRPPRDPVNAMLSFAYSLLTKEFAVTLETVGLDPM
                     LGFYHQTRFGRPSLALDLMEEFRPIVADSVVVGVVNNGVLKPDDFVVAPTGVAIASAG
                     RKKFIEAYERRMDQLVTHPVFGYRISYRRVIEVQARLLGRLLLGEIEAYPSFRTR"
     gene            2719..3699
                     /locus_tag="PK710_14115"
     CDS             2719..3699
                     /locus_tag="PK710_14115"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOT10909.1"
                     /translation="MGQGSAAETVAAIFLAFVEQRTWRQAELAQRIGVSVPWLRRRLL
                     SLQTAGMPLERDDETPQVYWSVPRGWFPGGIVLTGEQVVDLLPIVLRAPKSKRQQRVL
                     RLLAGSGLDVSSQQTVVAGTLTALEDSVLGVLEQGAATKTVTRLRYQSHSDAVAHWRH
                     VSVQRVMVGQPVRFVGLCHKATQLRWYRLDRVSAAELAGQEPWQSVSVEVLDKFISES
                     VGGFHGGETARTLCFSVRDPESRWVADNLPAGWQGKFTENGLEVQAQSASLHQVARFV
                     VSLGEAALCETNELQAEVQRLALGALHTSQARLNIHSVATNESSLWDSKS"
     gene            3730..6402
                     /locus_tag="PK710_14120"
     CDS             3730..6402
                     /locus_tag="PK710_14120"
                     /inference="COORDINATES: protein motif:HMM:NF037707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HOT10910.1"
                     /translation="MMVGEYQPWFEKIAGQNAKPHGWQQRLGESPCCENRLIRIPTGF
                     GKTLGVLSAWLFHRVVSNNDTWPRRLIWCLPMRTLVEQTLAEAEAACERAGLLWNGQG
                     SPQGLVGVYPLMGGMDGGQWHLWPDEYAILIGTQDMLLSRALNRGYGAGRARWPMDFG
                     LLSQDALWVMDEVQLMDVGLITSAQLQAFRQQDEKEKTCFRPCRTWWMSATLQPGWLE
                     SPDTHAWLPELPSLRIDSSSRTGPLWEVKKPLRLEKEVAQPRMLAAAVAQRHIELGSG
                     TQGPTLVVVNQVDRAVDVYQALRANRSLKNTDIRLVHSRFRPAERKRWREEFLCKQQC
                     QPGTNRIVVATQVVEAGVDFSAALLFTDLAPWASLVQRVGRVARWGGQGEVVVVDTAP
                     QNDGAAAPYTLDELEASRTALTQLADISPISLETFEESASPELVQSLYPYDPDHLLLR
                     HEIDELFDTAPDLSGADVDVSRFIRSGEERDVSVFWAEVPEKGVPSANRHPQRDALCS
                     VPFLRAQEWLCGKQTKTKRAPRLAKRRSAWVWDWLEGTWKEAERQDIYPGQVILVDAN
                     LGGYDWNEKTASGTGWNPTAPGPVKIVEGESTLPEDEADASQDNERLSLQAYRTIASH
                     SIDVATLARNTSTRLVPTLQDLFELAGRWHDAGKAHPAFQGSIRADGRPDRQDLAKAP
                     ENAWPRAFLYQSLQGDRRPGLRHEMASALALFAVLKEHEPLHPALLGPWKKLLEQLGT
                     QQQADKTASKPPTELEKRVLALDADSFNLLAYLVCCHHGKVRLSFHVSPSDQKAQAQG
                     GPLTIRGIRTGDRLADCLQGQPCDQAWLLDLSPSTVGLNPTTGMSWTERALGLLEDYG
                     PFALAWLEALMRAADQRASRKITKDPLLKGESLP"
     gene            6399..>7606
                     /gene="csx17"
                     /locus_tag="PK710_14125"
     CDS             6399..>7606
                     /gene="csx17"
                     /locus_tag="PK710_14125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csx17"
                     /protein_id="HOT10911.1"
                     /translation="MNIHQLRGCTPEPLAFYLKALGALRLVSEQVDSQARGFWKDECF
                     HLVTRLDADGLMNFFLHDYQPSPIVAPWNKGSGFYQTKDPGIYPVETSSSERFAPLRD
                     GIQAARRLIDEIAKADAEVRKIKDETKTGTSSERARLRSDPEYKQRLAKAERRFKDAK
                     QLLIPRCRKNWRGREREWFDAALVLDGDLTPVFPALLGTGGNDGRLDFTNNYYQRLAD
                     LFVLDSPEGKPQPSTRGWLLSALWGTPLPGAISGVVGQFMPGSAGGANTSNGPTGSAH
                     LNPVDFVFTLEGAINFRSAATKRLDGRSRVQASVPFAFPSNAAGYTTAAVSDEGGRGE
                     QWMPLWDQPLTYQELLHFLKEGRARLGSEQVQESLDFAQCIARLGTARGIVAFQRFGY
                     VERNGQSNLAV"
BASE COUNT         1698 a         2209 c         2110 g         1589 t
ORIGIN      
        1 caatggggcc gccgctaaaa gcagcggaaa atgtcgagat cgcgcgcgag catcttgatc
       61 gcatcgtcgc ttcaatgggg ccgccgctaa aagcagcgga aaataggcgg acgatgcacc
      121 gacgaccccg cggcggatca cctgcttcaa tggggccgcc gctaaaagca gcggaaaata
      181 cgtgtcgcag attacaccga aattcggtag cttgcaaggc attttgcgag agcccccgct
      241 ccgaggtccc ggcaagggct cctggaacag gttcgcttct tccgttttca aggacctgga
      301 taggggatcg agcacccccc agcttttcgt catcacacga ccgctcgaat cataccacga
      361 tagccagcaa aggcgcttgc ccatagggcc gcccgatcga agaaaaactc gtcatcgccc
      421 tgccatgcgc cggccccaca tccacaaata gtacttgatc ctcacggtga tgaatgatct
      481 cccctagctt tcctctcaat tccaatcgct ccatctccgt caactcacag cgaaacaccg
      541 aaagctgcac atgttcccca tagcctcgca tggtcttgta caccttccgc aacctcttcg
      601 ggtcgctgat gtcgtaggca accacgtagg tctgtctcat cgccgcctcc ttaccgcgtc
      661 cgaaatgatg gataggcttc gatttcccca agcaacaaac gccccagcaa acgcgcctgg
      721 acttcaatca ccctccgata gctgatccga taaccaaata ccggatgcgt caccaattga
      781 tccattcgac gttcataagc ttcgatgaac ttttttcttc ccgccgaagc aatcgccaca
      841 cccgtgggag ccacgacgaa atcgtcgggc ttgagcacgc cgttgttgac aaccccaacc
      901 accacggaat ccgccacgat gggtcgaaac tcctccatca gatccagcgc caacgaaggc
      961 cttccaaagc gagtctgatg ataaaaaccc aacataggat ccagacccac cgtctccaaa
     1021 gtgaccgcaa actccttggt caacagcgag taggcgaaag aaagcatcgc attgaccgga
     1081 tctcggggag gtcgacgatt gcgaccatcc aattcgaacg ctggttcgac cttcgacttc
     1141 aacattcggg taaattcacc gaaatacgtg cgcgccgccg ttccctccaa ccccaacaac
     1201 gactcgaggg cttcggctcg ctccgccttg tacgcaagct ggtccaattc cgacaacgct
     1261 tgcgccgtta ccccgatcgc gttgcgccgg agcatcgttc gactgttttt cactttgcca
     1321 accaccagcc ctcgcgccac cttcaggcag aactgcggat ccatcgccgc ccgcacctga
     1381 gccacccgaa gttccacgtt tttgctgctc aacccccgga cccggctata catccagccg
     1441 ccgtaggtaa aataagtcaa cggaatatcc cgctccatca aggctcgtat cgcctgggtc
     1501 gtgatctgca cattgcctaa caccatgacc tgcgacaaca tgggaagacg aacctcgata
     1561 gcagcccccg cctgccgccg cacgatgatc agctccccct cgacccggat ctgcgcccct
     1621 tgctcctgaa tataaagtgg cattttatca tctcgcgcag gatgaagccg tcgcgtcagg
     1681 gccgcttccc ccgtccccgc aagctgccag ggatccggct cgaggggtcc cgtcagctcc
     1741 ggatacaacg acaattgctg cgccaacgat gccggctccc cctcctcggg ccgatcgtgc
     1801 agtagcctcg tttcgtcggg caggcagatt cccaccagag aacacccctg gcacttttgc
     1861 tcatctttgg tgggaggagg tatctgtccc ttcgccgtga gttccttgat tcttcgcacc
     1921 gtgctcacca ccatcgccaa caaagaaggc tccaacgcga tgctcacacg gcgtcgagat
     1981 cccgcaaaat acagcgctgc ttcgttgcag atatacccat gctcccgcag caacatggcc
     2041 tgggcggcga gttgcgctcg ctccgggttt cgaatccaat cgtattccgg agccttgcca
     2101 cgcttgtatt caatcgggat gacttctcca ctctcgtttc cctgaacaac gtcgatcttg
     2161 gccgtgatcc ccagtttttc agaggacaac cacacagaac gagcggcata cgggatgtcg
     2221 tcgtccgaag cgggtgaatc ctcgatggac tcatccggac gcggagggag ccgaccagcg
     2281 ggctcatcgg cgcgacgatg cacgagcttt ccttccaccg tgaacgtgtt gtgggaaaac
     2341 tcgccctgca cccactcgag gtacatgagc ctctcgcaat acagggcttc gttgatcatc
     2401 cgcgcgggaa cgagttccgg tgcagacgaa ggaagttgcg gttcccacgg cagagttttc
     2461 gcccgcggtt gtttcgcggg acagggagat tgagctgaac tcaccttgtt ggacatgaat
     2521 cccctccatg gtcacgaaga ccggaccctt atactccgtt tggagtatgc cctattaccg
     2581 tcggaccgca aggatccggc ttcgaggcag caatttttcc ttggatactg tcgaaaacaa
     2641 ctagccatat ctcctatcct cccctatact tggccgccgt cattcgtcgg atcaattcac
     2701 gaggtcaagg ggcttatcgt gggacaggga agcgcagcag agacggtagc agccattttc
     2761 ttggcgttcg tggagcaacg aacgtggagg caggccgaac tggcccaacg aattggggtt
     2821 tccgttccat ggctccggag gcgtcttcta tccctgcaaa cggctggcat gcccctcgaa
     2881 agggacgacg agactcccca ggtctattgg agtgtgcctc ggggctggtt tcctggcgga
     2941 attgtgctga cgggagaaca agtggtcgat ctgcttccca tcgtgttgcg cgcgccgaaa
     3001 agcaaaagac aacaacgcgt gcttcgcttg ctggcaggat ccggattgga tgtgagttcg
     3061 caacaaacgg tggtggcggg aacactgacg gcgctcgaag atagtgtgtt gggagtattg
     3121 gaacaagggg ccgctacgaa gacggtgacg cggcttcgct atcaatcgca ttccgacgcg
     3181 gtcgcgcatt ggagacacgt ttccgtgcag cgcgtgatgg taggtcaacc cgttcgtttt
     3241 gtgggcctat gtcataaggc aacgcaatta cgttggtatc ggctcgatcg cgtgagcgcg
     3301 gcggaattgg ccggccaaga gccgtggcag agcgtgagcg tcgaggtttt ggacaagttt
     3361 ataagcgaaa gcgtgggagg cttccacgga ggggaaacgg caagaacgct ctgcttttcg
     3421 gtacgcgacc ccgaatcccg atgggtggcc gacaatcttc ccgcgggttg gcaagggaaa
     3481 ttcacggaaa acggtctgga agttcaggcg caaagcgcat ccctccacca agtggcgcgg
     3541 ttcgtcgtga gcctcgggga agccgccctt tgcgaaacca acgaattgca ggcggaggtt
     3601 caaaggttgg ccctgggagc cctgcatacg tcccaggcaa gactgaatat tcattcagta
     3661 gcgactaacg agtcgagtct ttgggacagc aagtcgtgat acgcttcttc caaccatgac
     3721 gtgggagtga tgatggtggg cgaatatcaa ccctggtttg agaaaattgc tggacagaac
     3781 gcaaaacctc atgggtggca gcaacgactc ggcgaaagcc cttgctgtga gaatcggctc
     3841 atccgaattc ccacaggctt cgggaaaacg cttggcgttc tttctgcctg gcttttccac
     3901 cgtgtcgtct ccaacaacga cacctggccg cgaaggctca tctggtgcct tcccatgcga
     3961 acgctcgtgg agcaaaccct ggccgaggcg gaagcggcgt gcgaaagagc tggtttgtta
     4021 tggaatggcc agggcagtcc ccaaggcctt gtcggggtat atccgctcat gggcggcatg
     4081 gatggcggcc aatggcacct ttggccggat gaatatgcca ttctgattgg cacgcaagac
     4141 atgctccttt cccgcgcctt gaatcgcgga tatggggccg gacgagcgcg atggccgatg
     4201 gacttcggac tgctcagcca ggatgccttg tgggtcatgg atgaggtgca actcatggac
     4261 gtaggcctca tcacgtcggc gcagcttcaa gcgttccgac agcaagatga aaaggagaaa
     4321 acatgctttc gtccgtgccg aacctggtgg atgagtgcaa ccctgcagcc cggctggctc
     4381 gaaagcccgg acacccatgc atggctcccg gagcttccct cccttcgaat cgactcctcg
     4441 tccaggacag gtcccttgtg ggaagtcaaa aaaccgttgc ggctcgagaa agaggtcgct
     4501 caaccccgta tgctggctgc tgctgtcgct caacggcata tcgagcttgg ctctggaacc
     4561 caaggcccga ccttggtggt cgtgaatcaa gtcgaccggg cggtggatgt ctatcaagcc
     4621 ttacgcgcga atcgcagctt gaaaaacacg gatattcgtc tggttcatag ccggtttcgg
     4681 cccgccgaac gaaaacgatg gagagaggaa ttcctctgta aacagcagtg tcagcccgga
     4741 accaatcgaa tcgtggtggc aacccaggtg gtagaagccg gtgtggattt ttcagcagct
     4801 ttgcttttta ccgacttggc cccctgggcg agtctggtgc agcgagtagg ccgcgtggcg
     4861 cgctggggag ggcagggaga agtcgtcgtg gtcgataccg ctccccaaaa cgatggtgcc
     4921 gcagcgcctt atacgctgga tgaattggag gcttctcgga cagcactcac gcaactagcg
     4981 gatatttcac ctatttctct tgaaacattc gaagaaagcg caagtccgga gcttgtccaa
     5041 agcctctatc cctatgatcc ggatcacttg ttgttgcgtc atgaaatcga cgaactcttc
     5101 gatacggctc ccgatctttc cggcgccgat gtggacgtga gccggttcat ccgctcgggg
     5161 gaagaacggg atgtctcggt cttctgggca gaagttccgg aaaagggcgt cccttccgcc
     5221 aatcgacatc cccaacggga tgccctatgt tccgtgcctt ttctgcgtgc ccaggaatgg
     5281 ttgtgcggca aacaaaccaa gaccaaaaga gcgcctcgtc tcgcgaagcg aagaagcgca
     5341 tgggtttggg attggctgga aggcacgtgg aaagaagctg agcggcagga tatttatccc
     5401 ggtcaggtca ttttggtgga tgccaacttg ggcggttacg attggaatga gaaaacagcc
     5461 tcaggaaccg gttggaatcc cacagcaccc ggtccggtca agatcgtcga aggggagtca
     5521 accctgccgg aggatgaagc agatgcttcc caagacaacg aacgtcttag tttacaggca
     5581 tatagaacga ttgcctccca cagcatcgat gtggccactt tggctcgcaa cacgtccacg
     5641 cgattggttc caacgctgca agaccttttc gaattggctg ggcgatggca cgatgcaggc
     5701 aaggcccatc ctgctttcca aggttccatt cgggccgatg gccgccccga ccgtcaagac
     5761 ctcgccaaag cgccggaaaa cgcctggccg cgcgcttttc tctaccaatc cctccaaggg
     5821 gaccgacgcc cagggttacg acacgaaatg gcaagcgcct tggccttgtt cgcagtcttg
     5881 aaagaacatg aacctcttca tccagccctg ctgggaccat ggaaaaaatt gctggagcag
     5941 ctcggcacgc aacagcaagc cgacaaaacc gcttccaaac caccgacaga actcgaaaag
     6001 cgtgtgctcg ctttggatgc ggattcgttc aacctgctcg cttacttggt ctgttgccat
     6061 cacgggaaag tacggctcag ttttcatgtg agtccaagtg atcaaaaagc ccaggcccag
     6121 ggaggtccgc tgacgattcg cggcatccgc acgggagaca ggcttgcgga ctgtctgcaa
     6181 ggacagcctt gcgaccaggc atggcttctt gacctttccc cctccacggt cggcctcaat
     6241 cccaccacag gcatgagttg gaccgagagg gcgctcggat tgctcgagga ttacgggcca
     6301 tttgctttgg cgtggctcga agcgctcatg agagcagcgg accaaagagc ctcacgaaag
     6361 ataacgaaag atcccttgct gaagggagaa tcgctgccat gaacattcac caattgcgag
     6421 gttgtacgcc tgaacccttg gccttttatc tcaaagctct tggagcgctc cggctcgttt
     6481 cggaacaagt ggattcccaa gcgcggggat tttggaaaga tgaatgtttt cacttggtta
     6541 caagactcga cgcagacggg ctgatgaact tttttctcca cgattatcaa cccagtccca
     6601 tcgtggcccc gtggaacaaa ggttcgggct tttaccaaac aaaggaccct ggcatctatc
     6661 cagtagaaac atcctcttcg gagcgattcg ctcctttacg cgatggaatc caagccgcaa
     6721 gacgactcat cgacgaaatt gccaaggcag atgctgaagt tcgaaaaatc aaggatgaaa
     6781 ccaaaacagg gacaagcagt gaacgcgctc gattgcgctc cgatccggaa tacaaacaac
     6841 ggttggcgaa agcggaacga cgcttcaaag atgcaaaaca gctactcatt ccccgatgcc
     6901 ggaagaattg gcgaggacgg gaacgtgaat ggtttgatgc ggcgcttgtt cttgatggcg
     6961 acctgactcc ggtgtttcct gcccttcttg gtaccggggg aaatgatggc agactggact
     7021 ttacgaacaa ctattaccaa cgtctggcgg atctattcgt gctggattct ccagaaggta
     7081 agccacagcc tagcactcgt ggatggctgc tctcagcatt atgggggact cccctgcctg
     7141 gcgcgatttc cggggttgtg gggcaattca tgcctggatc ggctggtggt gccaacacat
     7201 cgaacggtcc cacgggctcg gcgcacctca accctgtcga ttttgtattt accctggaag
     7261 gtgccatcaa cttccggagt gctgccacca aacgtcttga cggtcgttcg cgtgtgcaag
     7321 cttccgtgcc ttttgctttt ccgtcaaatg ctgctggata caccacggca gcggtgagtg
     7381 atgagggtgg tcggggggaa caatggatgc ccttgtggga tcaaccgctg acgtaccagg
     7441 agcttctgca ttttttgaag gaaggacgag cgcgccttgg ctcagaacaa gtgcaggaat
     7501 cgctcgattt cgcacaatgc atcgcacggt tgggcacggc tcgtggcatc gtagctttcc
     7561 aacgctttgg ctatgtcgag cgaaacggtc agtcgaacct ggcggt
//
LOCUS       DAOSOR010000260         7581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9690_length_7581_cov_4.561520, whole genome shotgun sequence.
ACCESSION   DAOSOR010000260 DAOSOR010000000
VERSION     DAOSOR010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7581
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9690_length_7581_cov_4.561520"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..487)
                     /locus_tag="PK710_14130"
     CDS             complement(170..487)
                     /locus_tag="PK710_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOT10912.1"
                     /translation="MTKSELIEFIAARAELSKARAEMVVNCVFDAMTEALRNGEGVEI
                     RGFGSLKARAHKSYQGRNPRTGKSVNVPPKLRPFFKVGKELKELVNASRSSGSIKDNG
                     DET"
     gene            complement(569..2659)
                     /locus_tag="PK710_14135"
     CDS             complement(569..2659)
                     /locus_tag="PK710_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10913.1"
                     /translation="MTPFNSILDVAWPADSAAWIAFFLGALVLATPWFLRAFPRLSLP
                     CWVGVWAFVSALLSVAYIHTYLRGGPRIIDATGYWLEARGFASGMVTWIADEPTAAIR
                     GRFLVHHPSAEGLRVGVLFPPGYPALLALGFLLHVPWLVGPILASAFTVSSCLLAHRL
                     TRRDDIVRIVAVLSALNACLRYHTADTMSHGLAAVLLTTSVVVALHASNHRWGYWLVC
                     GALTAWLAATRPATALALVVVGGPLVARFMLMHRRLAPLWSLLGMLPVFFLTVLHQHA
                     LTGAWFTSSQGLYYSLADGPEGCFRYGFGSGIGCLAEHGPYVASVAPDGFGLRAAFTT
                     TGRRLYLHLADLLNYAPFAIFLPLGVIQGWREKNTWLAVAMPLALIAAYAPFYFDGSY
                     PGGGARLLVDAMAFEHLLMALGLGAWGRAFRNPARGAHWAVAMATSLSLLGFALHGSR
                     MHQCLREREGGRPMYEPSVVRDALGAEPRGLLFVDSDHGFFLGFDPSATDPKRSLVVA
                     RARYDSRDYLRWNRLGRPPTYRYVYRYGQEESVVRIEPFMPTEPHGSYVFEAEAEWPA
                     LAQKGGYAVPTPLMAQPCIPSGRVLSLVRTGEEARVMTEIPVPSPGTYWIQGFFVANE
                     PVPSLRSRLWSEDMDVAMGVGAAGTATGDGRYCVATKRVPVHVFKKHIMWDVLTTDSW
                     VALDRVELQVLEAK"
     gene            complement(2656..4767)
                     /locus_tag="PK710_14140"
     CDS             complement(2656..4767)
                     /locus_tag="PK710_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10914.1"
                     /translation="MFRRFFSHILAESTLRRLSVIVYPLFSGWLVLAIARVFYGTMLE
                     ATGGEWSAPLDDVFIHFDFARSTARGYPFQWSEGNGYSSGNTSLTYPFILAFGYWVGF
                     RDARLMMWAAIVACCCIFALLMLIPRLARGLPFAARFLLPIGVFSVGALSWSLFSGME
                     VAWFLAVWAIALSYALNHRHGSARQRPGFGWKLGLAGAVLVATRPEAATSIAVLGLTA
                     AIFVARSTSRLRPALATLVRAGAPSIATLVIQALVNRWLTGDFAAAGARVKLAWYHPY
                     MPPQEKWETYLDHLKYSFLRNIDYHFSVDPRIGWIPVVLAVIALLSARTRTSAAILLS
                     SAVSFLCLVAMNGQVRWQNERYTMPAVAWLLIAASLGLGVLLFRRPSRLPWFTSVPRV
                     VIALALVITFGVCQAPRMRDQIHFFAKASKNIRDQHITTGRLLRHHIHPTPQRILVGD
                     AGAIMYASDLPGLDIIGLGGFHGMPFARASTHGLAAIIELIERIPNQDRPDIFAIYPS
                     WWSTLPLWFGKQIAAVPVRDNVICGGDEKVIYAADWTLLNTGELPFSLDDNEIITDWL
                     DVADLINEHEHDYRFPSPDAGYVELRILTEPTQGRDVLDGGRRIPENGTESFRLTSQR
                     LASSMRLIVRTAPAFPGKVEVSMDGHFIGELTLEPSDTWVELSLPLPSPLKPNQTATF
                     TMTARSTSRWVNYHVWLVASP"
     gene            4934..6706
                     /locus_tag="PK710_14145"
     CDS             4934..6706
                     /locus_tag="PK710_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10915.1"
                     /translation="MRWFVEINSLGKPEQKAVRSCLEASQWSVAYQEIRRSSGQDDSL
                     GYLSIELLDDGVRIVDPKSRQRYEIRRAPDDAAVTVANTENTVTTASERVSERPSEPK
                     PLSQPPAHMPSATPLARPYSRAPSVPPPTTQPRIPSVPPQAPPHVKTPSVPPPAPAVP
                     QPIPTPPPPAGLAPIPNEPYQVLARREEQPSKTSPLTYREYAYAIAEGSTEKEAETFI
                     FQRFEEIKDELASTRSAKLVNLAVFDHAFSDRPKRPPLVTLVWKDWKGNPEIRYTSRP
                     GVSIPPPSLGRPSSQPAAEPPSVASNPPQPIETPSAVSSLPKELPSSVPPAAPSHEKE
                     VSTAATDEQPARSRAADSSSPSSDSSHYTPASTPVAKRQRRSDAPQPATASSGQAAEK
                     AQPVSRSEKVPPSPLSDRQARKSSQSGEIIADIFESMHDLYFLRDAYEGADFVIALAL
                     DKMRCTLGMAQLYDINRREFVVAHVVGPNAQDTLGHRTSEHDPLLVEIMNRRLPFIMQ
                     GTDPRIDRGRWKQLGGSPHSILACSVAQGGRFLGILEVAHEDRKKTFRKADVDAMAYI
                     AQSFAEFIAKIGLQFGSVTPFNPK"
     gene            6719..>7581
                     /locus_tag="PK710_14150"
     CDS             6719..>7581
                     /locus_tag="PK710_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOT10916.1"
                     /translation="MAERTRREVLLTFLLTPAVVLGVVALAVITFRTTFQIETLRQQS
                     VLEATVSVANEKADRLDKRIIEEDNVVVAEADVTILGSLARRWLPTASRETPTVRAVV
                     IMRADGDHDVYDFATQNPGPEDDAFRRMLVHRLLPHLDLQQGAAEQLRHLHRTLDGRS
                     YLISYWQREHEGRKYLVVAWHDVPRIVHDLFPRMLEDATGHARVHVVDEEGRTVYGQP
                     TVGGVFTVGRPFETTLYGWRLYATLTTAEELSESVERRRELEMVSVAVSFLVVAAGVI
                     IVVVAAERERR"
BASE COUNT         1785 a         2298 c         2110 g         1388 t
ORIGIN      
        1 aggctcgagt tgcaaaggtt cgtgctgcga cgacggttcg atgactttcg aggacgacga
       61 tatcgccggc tgacacgcgc tcgcatacac cgatgccagg ctgccaagcc acgcaagcaa
      121 gtgcgcgccg aaatcacgac gcgtcgccgc accgagcgtc atccgactct taggtctcgt
      181 cgccgttgtc tttgatgctc cccgacgagc gactcgcgtt gaccaattct ttgagttcct
      241 tgcccacctt gaaaaagggg cggagcttgg gcggaacatt gaccgacttg cccgtgcgag
      301 ggttgcggcc ctggtaagac ttatgcgccc gggccttgag actgccaaag ccccgaatct
      361 caacgccttc tccgtttcga agcgcctcag tcatggcgtc gaacacgcag ttgacgacca
      421 tttccgcacg tgccttgctc aactccgcac gcgctgcgat gaactcgata agctcactct
      481 tcgtcatggg tgccattccg tgggcatagt tgtcgccctg atcgaaacgg cactgtatca
      541 cgagccgtcc tttcgcaagc cagaaaagtc acttagcttc caatacttgc aactcaaccc
      601 gatccagcgc cacccacgaa tccgtagtga gcacgtccca cattatgtgt tttttgaata
      661 catgaaccgg gacccgcttc gttgccacac aatatcgacc gtcccccgtt gccgttcccg
      721 cagcacccac ccccatcgcg acatccatat cctcggacca gagccgcgac cgaaggcttg
      781 gaacaggctc atttgccaca aaaaatccct gtatccaata agttccaggc gatgggaccg
      841 gaatctccgt catgacacgg gcttcttcgc ccgttcgcac caaagatagg acacgtccgc
      901 tgggaatgca tggctgcgcc atcaacggag tcgggaccgc atatccccct ttttgtgcca
      961 acgcgggcca ctccgcttcc gcttcgaaaa cgtaggaccc gtgaggttcg gtgggcatga
     1021 agggctctat tcgtaccacg gattcctctt gcccatatcg atacacgtat cggtaggtcg
     1081 gtggacgtcc caaccggttc catcgaagat aatcgcgaga atcgtagcgc gctcgggcaa
     1141 cgaccaacga ccttttcggg tcggtagcac tagggtcgaa gccgagaaaa aaaccgtggt
     1201 ccgagtctac gaaaagcagt cctcgtggtt cggcgccaag agcatcacgc accaccgacg
     1261 gctcgtacat gggtcggccg ccctcgcgct cccgcaaaca ttggtgcatt cgactgccgt
     1321 gcagcgcaaa gccgagcaga cttagcgaag ttgccatcgc caccgcccag tgtgctcccc
     1381 gggctggatt gcggaacgct cggccccaag ctccaagccc aagtgccatg agcagatgct
     1441 cgaaagccat cgcatcgacc agcaatcgag cacctccccc cggataggaa ccatcgaagt
     1501 aaaacggtgc gtaagcagcg atcaatgcca agggcatcgc cacggccagc caggtatttt
     1561 tctctcgcca cccctggatc acgccaaggg ggagaaatat cgcgaaaggc gcgtagttca
     1621 gcaaatccgc cagatgcagg tacaaccgcc ttccggtcgt cgtaaaggca gctcgtaggc
     1681 caaacccatc cggtgccacg gaagcaacgt agggaccatg ttctgccaaa cacccaattc
     1741 ctgatccaaa accgtagcgg aaacagccct cgggcccatc ggctagcgag tagtacagcc
     1801 cctgactgct ggtgaaccac gcccccgtca gcgcatgctg atgcaacacg gtcaaaaaaa
     1861 atacgggcag cattcccagc agcgaccaca acggtgccaa gcgccggtgc atgagcatga
     1921 agcgcgcaac caagggaccc ccaaccacga caagagcgag agccgtggct ggacgggtgg
     1981 ctgccaacca agccgtaagc gctccacaaa ccaaccaata tccccatcga tggttcgacg
     2041 catgaagggc caccaccacg gaagtggtta gcagcacggc cgctagcccg tgagacatgg
     2101 tgtccgcggt atgatagcga aggcaagcgt tgagggcgga taacaccgca acgatgcgaa
     2161 cgatatcgtc tctgcgtgtc agccggtgcg cgagcaggca tgacgacacg gtgaaagcgc
     2221 tggccaggat tgggcccacc aaccaaggga cgtgcagcag gaatccgagc gccaacagtg
     2281 cgggatagcc agggggaaag agcacaccga cacgaagtcc ctcggcgctt gggtggtgga
     2341 caaggaaacg acctcgaatg gcggcggtgg gctcatcggc gatccatgtc accatccccg
     2401 aagcaaagcc gcgagcctcg agccaatacc cggtagcatc gatgatgcga gggcctccac
     2461 gaagataggt gtggatgtag gccacggaca gcaaggcaga tacaaacgcc caaacgccaa
     2521 cccagcaggg gagcgaaagc cgcggaaaag cacggaggaa ccatggggtt gccaacacca
     2581 aagccccaag gaaaaacgcg atccacgccg ctgaatccgc gggccaagcc acatcgagga
     2641 tcgagttgaa cggtgtcacg gggaagcgac caaccagaca tgataattca cccagcgcga
     2701 agtcgatcgc gccgtcatcg tgaatgtcgc ggtttggttt ggtttcagtg gagaaggtaa
     2761 cggtaacgac aattcgaccc acgtatccga aggttcgaga gtcaactccc cgatgaagtg
     2821 gccgtccatc gatacctcga cttttcccgg aaaagcaggg gcagtccgca cgatcagacg
     2881 catcgacgag gccagccgtt gggatgtcaa acgaaaagac tccgtgccgt tttcgggaat
     2941 tcgacgccca ccgtcgagca cgtctcgtcc ttgggtcggc tctgtcagga ttcggagttc
     3001 gacgtaaccc gcatctgggg aaggaaaacg ataatcgtgt tcatgctcat tgatcagatc
     3061 ggcgacgtcg agccaatcgg tgatgatttc gttgtcatcg agggagaaag gcagttcacc
     3121 cgtgttgagc aacgtccaat ccgcggcgta gatgaccttt tcgtcaccgc cacaaatgac
     3181 attgtcccgc acgggaacgg cagcgatttg ttttccgaac cagaggggca gggtgctcca
     3241 ccaagaggga tagatggcga aaatatcggg acggtcttgg tttgggattc gttcgatcaa
     3301 ttcgatgatg gctgcgaggc cgtgggtgga agcgcgggcg aacggcatgc cgtgaaaccc
     3361 tccaaggccg atgatatcga gccctggtaa gtccgaggca tacatgatgg ctcccgcatc
     3421 tccgacgagg atacgttgtg gagtggggtg gatatggtgt cgaagtagac gaccggtcgt
     3481 aatgtgttga tcgcgaatat ttttagaggc ctttgcaaaa aagtggatct ggtcgcgcat
     3541 gcgcggtgct tggcacacac cgaaggtgat gaccaacgcc agggcgatga ccacccgtgg
     3601 caccgaagtg aaccacggca gccgcgaagg acgtcgaaat agaaggactc ccagccccaa
     3661 ggatgcggcg atgaggagcc aagcgaccgc tggcatggtg tagcgctcat tctgccatcg
     3721 gacttgtccg ttcatggcaa cgagacaaag gaaggagacg gcggaagaaa gcaggatggc
     3781 ggcggaagtg cgggtacgag ccgagagcaa tgcgatgacg gccaaaacga ccggaatcca
     3841 accgatacga ggatcgacgg agaagtggta atcgatattt cgcagaaaag agtatttcag
     3901 atgatcgagg taggtttccc acttttcttg gggtggcatg tacgggtggt accaagcgag
     3961 tttgacgcgg gcacccgcgg cggcgaagtc accggtgagc caacggttga cgagagcctg
     4021 aatcaccaag gtggcgatgg agggggcacc tgctcgtacc aaggtagcca aggcgggtcg
     4081 caaccgtgag gtggagcgag ccacgaaaat ggcggctgtc aatccgagca cggcaatgga
     4141 agtggccgcc tccgggcgtg tagccacgag aacggctccc gccaatccga gtttccatcc
     4201 aaaaccggga cgttgccggg cggagccatg acgatggttc agggcatacg atagggcgat
     4261 ggcccacacc gcaagaaacc aggcgacttc catgccgcta aacagtgacc aggacaacgc
     4321 gccgacagaa aaaacaccga tgggcaaaag aaacctggcg gcaaagggca ggccgcgagc
     4381 caagcgtgga atgagcatca acaatgcgaa aatgcaacag caagccacga tggcggccca
     4441 catcatgagt cgcgcatctc gaaaaccaac ccaatagcca aacgcaagaa tgaacggata
     4501 ggtcagactg gtgttgccgc tcgagtaacc attgccctcc gaccattgga atgggtaacc
     4561 gcgcgccgtc gatcgagcaa aatcgaagtg aatgaacaca tcatccaacg gtgcggacca
     4621 ctcacccccg gtagcctcca gcatggttcc gtaaaatacc cgggcgatcg ccaggaccaa
     4681 ccatcccgaa aacaggggat agacaatcac cgagagacga cgaagcgtcg attcggccag
     4741 gatatgggaa aaaaaacgtc gaaacatcga tagcgggtaa gtcttttcat agacacaatg
     4801 agccgcattg aacataaata gcgcgcgtcg ggctcatctt gtcatcgtgg cttcgcgaac
     4861 accgttgacc ctttctgaag cactcggtac accattcggc gcggcgtgtc gcccccttgg
     4921 tcgtagaggc cccatgcgtt ggttcgtcga gatcaattcc ctgggaaagc cagagcaaaa
     4981 agcggtccgt tcctgcttgg aagcttccca atggtccgtg gcatatcaag agattcgccg
     5041 ttccagcggg caagacgatt ctttgggata tctttccatc gagttgctgg atgatggtgt
     5101 gcgaatcgtc gatccaaaat cgcgtcagcg atacgaaatc cggcgcgcgc cagacgatgc
     5161 tgccgtcacg gtggcaaaca ccgaaaatac tgtgactacc gcctcggaac gtgtttccga
     5221 gcgtccttcg gaacccaagc cactgtccca gccgcccgcg cacatgcctt ccgcgacgcc
     5281 tttggctcgg ccctattccc gtgccccatc ggttccgcct cctaccacac agccacgaat
     5341 tccttccgtt ccgccgcaag caccgcctca tgtaaagact ccttcggttc cacccccagc
     5401 tccggctgtt ccgcaaccaa tccccactcc acccccgcca gcagggctcg cacccatccc
     5461 caacgaaccc tatcaggtgc tcgccagacg cgaagaacaa ccgtccaaaa cctctccgct
     5521 cacgtaccgc gaatacgcct acgccattgc cgaaggttcc accgaaaagg aggcggagac
     5581 cttcattttc caacgtttcg aggaaatcaa agacgaactg gcctccactc gctcggccaa
     5641 actcgtcaat ctcgccgtat tcgaccacgc cttttccgat cgacccaaac gcccgccgct
     5701 ggtcacactc gtgtggaaag attggaaagg caatccggaa atccgttata cctcacgtcc
     5761 cggcgtgtcg atcccgccgc catcgctcgg gcgcccctcg tctcaacctg ccgccgagcc
     5821 cccatccgtc gcctccaacc caccgcaacc catagaaacc ccatcggcag tttcgtcact
     5881 accgaaagag ttaccatctt ccgtcccccc cgcggcccct tcccacgaaa aagaagtctc
     5941 cactgccgca acggatgaac aacccgctcg ctcccgtgcc gcggatagct cttcgccatc
     6001 ctctgattca tcgcattaca ccccagcttc cacaccggtg gccaaacgcc aacgtcgaag
     6061 cgatgctccg caacccgcca cggcatcctc gggtcaagcg gcggaaaaag cgcaaccggt
     6121 ttcccgatcg gagaaagtgc cgccttctcc cctatccgat cgacaagctc gtaagtcatc
     6181 gcaaagcggt gagatcatcg ccgatatttt cgaatcgatg catgatcttt actttttgcg
     6241 agatgcctat gaaggcgcgg atttcgtgat cgctttggcg ctcgataaaa tgcgctgcac
     6301 cctcggcatg gcccaacttt acgacatcaa tcgccgcgag ttcgtcgtgg ctcacgtcgt
     6361 cggtcccaac gcgcaagaca cgctcggcca tcgtacgagc gagcatgatc cgctgcttgt
     6421 cgagatcatg aatcggcggc ttcctttcat catgcaaggc acggatccgc gtatcgatcg
     6481 aggtcgttgg aagcagcttg gaggttctcc ccacagcatt ctcgcttgct ccgtggcgca
     6541 aggagggagg tttttgggga ttctcgaggt ggcccacgag gatcggaaaa aaaccttccg
     6601 aaaagccgac gtggatgcca tggcctatat cgcacagagc ttcgcggaat tcattgccaa
     6661 gattggcctt caattcggca gcgtgactcc gttcaatccg aagtgaccga gcttttcgat
     6721 ggccgagcgg acccgtcgag aggtgttact caccttctta ctcactcccg ccgtggtgtt
     6781 gggtgtggtc gcgctcgctg tcattacctt ccgtaccacc ttccagattg aaacccttcg
     6841 acagcaatcc gtgctcgaag ccaccgtgtc ggtcgcgaat gaaaaagcag accgcctcga
     6901 caaacgcatc atcgaagaag acaacgtcgt ggtggccgaa gccgatgtca ccatcctagg
     6961 ctccctggct cgtcgttggc tccccaccgc ttcgcgcgaa accccaaccg ttcgagccgt
     7021 cgtcatcatg cgagccgatg gcgatcacga cgtatacgat tttgcgactc agaatccagg
     7081 acctgaagac gatgcatttc ggcgcatgct cgttcatcgt ctcctgcctc atctcgactt
     7141 gcaacagggg gctgcggaac agcttcgtca ccttcaccgc acgctggatg gacgctcgta
     7201 tctcatcagc tattggcaac gagaacacga gggcaggaaa tacctcgttg tggcatggca
     7261 cgatgttcca cgcatcgttc atgacctatt tccccgtatg ctcgaggatg ccaccggaca
     7321 tgctcgcgtc catgtcgtcg acgaagaagg cagaacggtg tacggacaac ccaccgtggg
     7381 tggtgtgttt acggtgggac gcccgttcga aaccacgttg tatggctggc ggttgtacgc
     7441 aaccttgacg accgcggaag aactgagcga gagcgttgaa cgacgacgag aattggagat
     7501 ggtttccgtg gcggtttcat ttctcgtggt ggcggcgggg gtcattatcg tggtggtggc
     7561 tgcggagcga gagcgacggt t
//
LOCUS       DAOSOR010000261         7561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9728_length_7561_cov_4.447242, whole genome shotgun sequence.
ACCESSION   DAOSOR010000261 DAOSOR010000000
VERSION     DAOSOR010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9728_length_7561_cov_4.447242"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..1469)
                     /locus_tag="PK710_14155"
     CDS             complement(252..1469)
                     /locus_tag="PK710_14155"
                     /inference="COORDINATES: protein motif:HMM:NF017442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HOT10917.1"
                     /translation="MLTSDLIRVRRRGQRIEPLVLSPRQFQAAIPLAEALINVFQSNI
                     GSTMEELDEALRKVADAEPNRMQVAGFIKLLRDRCEIGTAPECDPAALRAEVFRLATQ
                     QRRALGIREPFHRSAVIDACAARHHCTPEQLESMLFADLPGAQLLSSMQPITPIDLLH
                     RYNLALAQGILLRSTQVTIHLVPTTATRIRQLIRAMRFRRLMFQITGSAQSGYEIVLD
                     GPMSLFESTQRYGIQLALFLPALVAEEGWSLKARLRWGKERKEAVLSISDQDGFVSPY
                     RKDSTELEEISTLVESFSRLDCPWSVRRSSRIFHLQNQSLFVPDLVFEKDNKLRVYLE
                     AFGTWNRDAVFDRVQILQQELGERFILAVSKKLRVSPEIACDDFPLRILVYGSAISAH
                     SVWRLLEDIVKDV"
     gene            complement(1560..2759)
                     /locus_tag="PK710_14160"
     CDS             complement(1560..2759)
                     /locus_tag="PK710_14160"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOT10918.1"
                     /translation="MRVSNLVTVTLSCLCSIALGCGDDTTHHEGGGDAATPPNTGGQT
                     NTGGQAGAGGSTSTGGQAGAGGSTSTGGQAGAGGGGPTAGWLYTIDGDPHIYLDGGGS
                     TGVWMGRGVNLDDLFLCGYNYGLWMDSPHDGEHALLGLIDAMMASWKPNFVRVSLGMN
                     SYSPVVHWQAGSDYTTAMTNVIEHLGSYAGVFVLVSLRSDTTMVEPGGGECGQGDDAV
                     CLPSNATDDVYRALVQSFHDKPYVLFGIANEPGGNGSSNSDIRARMEHAVSVIRAQED
                     ALGSPHHLIAVQGNQWTSQIAFYAADPLSFDNVVYEYHAYPPIASEYTFSNIPVIIGE
                     YGPSGSDTSFSLGFFADVEAKQIPNLAWSLSPYSNCAPDLVAVTHDASLTTTAWGDVV
                     KAYLQAH"
     gene            complement(3201..3533)
                     /locus_tag="PK710_14165"
     CDS             complement(3201..3533)
                     /locus_tag="PK710_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10919.1"
                     /translation="MTIARNKIYEGKSGLSLGGQGGIYPFGGVRDLAIFENVFQVDSD
                     AIVLNGDSNQTKRFQVWDNTGSSSYKRPDWVSGDYWASNADGASYGSRNDQPWMSEQF
                     AWEAFDRY"
     gene            complement(3505..5505)
                     /locus_tag="PK710_14170"
     CDS             complement(3505..5505)
                     /locus_tag="PK710_14170"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOT10920.1"
                     /translation="MRRDVDVQLRNLAQVNEITRREAKRGNAVIFAWIAASLLNLACS
                     SDRTGDGANAAGGNADAGVSGAGGAGPQKPPQNDGGDAWHETGDAGEAGFSGTGGTGE
                     AGVAGAGGTGGAGGTEEQHLPQIPCAHSSAPGFGSVGDENPNQGPRSPGHVVAAQGPT
                     QAESRYALITWDRNDSSATGYEVLRDGNVIASLKIEQDAWDDTCYLDMKVTPGVHEYA
                     VRALRGTTPGKRSASYRISILQDADFGSIFEVDSFGGSEDSDRIEAAIEAAQTAGGGV
                     VQFAPRTYTLSRPIVVAGNRIVLRGAGMDDTFLQPSYPGASGDDACASTERIVYFNTK
                     LSDLGNLLAAPIQQGERVVNVTDSSALAVGNILYFSEVQPEQTPADFQNAGIIQDPGT
                     GKDQRYPNESNEIVAIDGLQLTFKYPFSHDFSMNTPLQRFERGLDNRIESLTIQGRSE
                     NEQTWYDGLNLKGVNGHAADVRVRWTNRRLVQVQGHGTRLVGFHGPYGGPRGGESGIC
                     RYKIHVYRATNALIVGSTMGMPNDDRNLSFITIQQSTRGMVRNSIFLKNQTYGVNEHG
                     KGSRHLLVENNYFSVGTPNRAGVLLGNESWGFSGPMIIRNNTFEDNDRDIHITQNSYE
                     VRMLDNISRRNGRQFLRASGWAGYGTGVLRFDAHDHRAQQDL"
     gene            complement(5910..6533)
                     /locus_tag="PK710_14175"
     CDS             complement(5910..6533)
                     /locus_tag="PK710_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10921.1"
                     /translation="MKEVRTTVKALLIVFVGLGCGGIAETSGSDGEGGQAGKGGTGGK
                     AGSGGAPSGGSGGIGGSGATGGSGGMPTGGTGGIGGNGAMGGSGGMPTGGSGGSLSQC
                     ESEGGVCIAVAVTPNACVQCPSTTGQLLVPAPYPDTTRGCTTEGVGASPWCCVTVPPV
                     GDCVSSGGACYPITETAWEPCPLGWDPIYTPCPNGDICCAPGPGCEP"
     gene            complement(6713..>7561)
                     /locus_tag="PK710_14180"
     CDS             complement(6713..>7561)
                     /locus_tag="PK710_14180"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT10922.1"
                     /translation="APAPPRHLSGKHHLPGESYIYIARTSIAPFRLGLTATLERADGR
                     HALLDDYLGPVVYRIEIPELSGSYLADYETITLQVELDAAERDEYERARKVYTSFVRS
                     RNISFSASDGWRQFLIESSRCAEGRQAFQAYRRQKELAQGAVGKLRVLEGLLHQHRQE
                     RMLIFTQDNATAYAISRRFLVPIITHRTKPKERAAILDGLRQGEYCAVVTSKVLNEGV
                     DVPEANVAVVLSGSGSVREHVQRLGRILRRAEGKQAVLYEVVSSSTAEQWVSQRRRDH
                     GAYRGG"
BASE COUNT         1642 a         2292 c         1990 g         1637 t
ORIGIN      
        1 caccgtggga aagtgcgcgg cctggcatgg atgaggatcg aaaacagcgg tcgatataat
       61 tcccctatag ccgaccattc gttatcaccc aacgcaaacc agtccctccc atgaccccag
      121 ggatttccct cgtacgaggg tgctgttgga gatgatcgac gcaacaaagg gcttcaggtt
      181 gatgaaggtt cgtctgcctg gggatcatcg gcagaaatcc gctggcgttt tcctcgccct
      241 cctggaaacc gtcagacgtc tttgacgata tcttcgagca atcgccatac cgaatgggcc
      301 gagatggcgg agccatacac caagatgcgc aatgggaaat catcgcaagc gatctccggg
      361 ctcacgcgca attttttgct gactgcgagg atgaatcgct cgcccaattc ctgttgaagg
      421 atctgaacgc gatcgaaaac cgcgtcccta ttccaggttc cgaaagcttc gaggtagacg
      481 cgcagcttat tatctttttc gaaaaccagg tccggaacga atagactctg gttttgcaag
      541 tgaaaaatgc gcgaggagcg tcgcacggac catgggcagt cgaggcgcga gaaggattct
      601 acgagagtgc tgatctcttc gagttccgtg gagtcctttc ggtagggtga tacgaagcca
      661 tcctggtcgc tgatgctaag caccgcctcc ttacgttctt tgccccaacg aagcctggcc
      721 ttgaggctcc atccttcctc ggccacgagc gcaggaagaa ataaggccaa ttgaatgccg
      781 tatcgctgcg tggattcgaa tagactcatg ggaccatcca gcacgatttc gtatccggat
      841 tgtgctgagc ccgtgatttg aaacatcaat cgacgaaacc tcatggcacg gatgagttgt
      901 cgaatgcgcg tggccgtggt ggggacgagg tgaatcgtga cctgggtgga gcgcagcaag
      961 atgccttgcg ccaaggcgag attgtaccga tgcaacagat cgatgggcgt gatgggctgc
     1021 atactcgaaa gaagctgtgc tcccggaaga tccgcgaata gcatggactc gagttgttca
     1081 ggcgtgcagt ggtgccgggc cgcacaagca tcgatgaccg ccgaacggtg gaacggttcg
     1141 cggattccaa gcgcgcgccg ctgttgcgtg gccaagcgaa acacctccgc gcgcaacgcc
     1201 gccggatcgc attccggggc tgtcccgatc tcacaccgat cacgtaataa cttaataaat
     1261 ccagcaactt gcatacggtt gggctctgcg tccgcgacct tgcgaagagc ctcgtccagc
     1321 tcttccatgg tggagccgat gttgctttga aaaacgttga tcaacgcttc cgcgagaggg
     1381 atggccgcct gaaattgcct gggagacaaa accagcggct cgatgcgttg accgcgtctt
     1441 cgaacacgaa tgagatcaga ggtgagcaaa ccagcttacg tgtaatcgtc aaaccctcac
     1501 cgcgtcaatc ccaaggccat ggcagggcaa gacgaccgca tttcattccg ccgcgaccgt
     1561 cagtgcgcct gaagataggc cttcacgacg tctccccacg cggttgtcgt cagcgaggca
     1621 tcatgcgtca ccgcaacgag atcaggcgcg caattcgagt agggcgacag ggaccaggca
     1681 agattcggaa tctgcttggc ctcgacatct gcgaaaaatc ccagtgaaaa tgacgtgtcc
     1741 gaaccacttg ggccatactc gccaatgatg accggaatgt tcgaaaacgt gtattcggag
     1801 gcgatgggcg gatatgcatg gtattcgtaa acgacgttgt cgaaagacag cggatcggca
     1861 gcgtaaaacg caatctgact cgtccactgg ttgccttgca ccgcaatgag gtgatggggg
     1921 gaaccgaggg catcttcttg cgcacgaatg accgaaaccg cgtgctccat ccgcgcacga
     1981 atatcgctat tcgacgaacc attgcccccc ggctcgttcg caatcccaaa caggacataa
     2041 ggcttgtcat gaaacgactg aacgagcgct cgatatacgt cgtccgtcgc gttcgacggc
     2101 aggcatacgg cgtcgtctcc ttggccgcat tcacctccac ccggctcgac catcgtggtg
     2161 tcactgcgaa gcgatacgag cacgaacact cctgcatacg agcccaggtg ctcgatgacg
     2221 ttcgtcatgg ccgtggtgta gtcactaccg gcttgccaat gcacgacagg gctatagctg
     2281 ttcatgccga gggagacgcg gacgaaattg ggtttccacg aggccatcat ggcgtcgatg
     2341 aggccaagca aggcgtgttc gccatcatgg gggctgtcca tccaaaggcc gtagttgtac
     2401 ccgcataaga acaggtcatc gaggttgacc ccgcggccca tccacacgcc cgtcgacccg
     2461 cccccgtcga ggtaaatatg cgggtcgccg tcgatggtat aaagccagcc cgcggtaggt
     2521 cccccgcccc ccgcgcctgc ttgacctccg gtgctcgtgc ttccccccgc gcccgcctgg
     2581 ccgccagtac tggtgcttcc ccccgcgccc gcctggccgc cagtgtttgt ctgcccgccc
     2641 gtgttaggag gagtggctgc gtcgccaccg ccttcgtggt gagtcgtatc gtccccgcag
     2701 cccaaagcaa tactgcaaag gcacgagagc gtaaccgtaa ccaagttgga aacccgcatt
     2761 ggttctactt taccgcaagc ggcaatcaga ggcgagcagg gaaatcgaac agcttttacc
     2821 gctgagccct cccgtcgttt ttccaatcac ggtcacggca taggcaatac actcacagtc
     2881 atcccctgtt gaacaccgca atgcgctcgc ctcttgtttt atagcatatc gtgtcatcgt
     2941 actccgatga ggcgcttgac caaaaccatc aaagcaaacc cgtccagcta aagtgtaaac
     3001 gagccctgtc agcataggta ccaaaagtgt aaactatgta ttgtcttgga ccttcaagtt
     3061 catgtcaagg accaggtcaa actcaaagtc aacgtcaagg tcaaagctta ggggattgtc
     3121 gcccatatcc cttgtttttc aggaaaaaat gaggggatct atcagggaca tgagagcgcc
     3181 acgtaaaaac acgggctgac tcaataacgg tcaaacgctt cccaagcaaa ctgttccgac
     3241 atccatggct gatcgttacg gctgccgtac gacgcgccat ccgcattgct tgcccaataa
     3301 tcaccactca cccaatcagg ccttttgtag ctcgaactgc ctgtattgtc ccaaacctgg
     3361 aaccgtttgg tttggttgct gtcaccgttc agcacaattg catcgctgtc cacttggaaa
     3421 acgttttcaa aaatcgcaag gtctcgcacg cccccaaagg gatatatacc gccctgccca
     3481 cccagactaa gccctgactt gccttcatag atcttgttgc gcgcgatggt catgcgcatc
     3541 gaaccgtaat actccggttc cgtatccggc ccatccggaa gcgcgcagaa attggcgtcc
     3601 gtttctccgc gaaatattgt ccagcatgcg gacttcgtac gagttctggg tgatgtgaat
     3661 atcgcgatca ttatcctcga acgtgttgtt gcggatgatc atggggccgc tgaatcccca
     3721 tgactcattg cccaataaca ccccggcccg attcggggtt cctaccgaaa aatagttatt
     3781 ttccacgagc aaatggcgag atcccttccc gtgctcgttc acgccataag tttgattttt
     3841 caaaaagatc gaattgcgaa ccatgccacg agtcgactgc tggatcgtga tgaacgacaa
     3901 attcctatca tcgttgggca ttcccatcgt agagccaaca atcaaagcat tcgtcgcccg
     3961 gtaaacatgg attttgtaac ggcaaattcc cgattctcct cccctcgggc caccataagg
     4021 cccgtgaaaa ccaacaagac gcgttccatg accttggacc tggaccaacc gacgattcgt
     4081 ccatcgaacc cgaacgtctg cagcatgccc gttgactcct ttcaggttca gtccgtcgta
     4141 ccaggtttgc tcgttttcac ttcgcccttg aatcgtgagc gactcaatcc gattgtcgag
     4201 tccgcgttcg aatcgctgca agggcgtatt catgctgaag tcatgggaaa atgggtactt
     4261 gaacgtaagt tggagcccat cgatggcaac gatttcgttg ctctcattgg ggtagcgttg
     4321 gtctttgcct gtccccggat cttggatgat ccctgcattt tggaagtctg ccggtgtttg
     4381 ctccggctga acttccgaga aataaaggat atttccaacc gccagggccg aactatctgt
     4441 cacgttgaca actcgttccc cctgttggat gggagcagca agaaggttgc cgagatcact
     4501 tagtttcgtg ttgaaatata cgattctttc ggtgctcgca caggcgtcat cgccggaagc
     4561 tcctggatac gatggctgca aaaaggtatc gtccatgcct gctccacgca gaacgatacg
     4621 gttgcccgcc accacgatgg gtcgtgacag cgtatacgtg cgcggcgcga attggaccac
     4681 gcccccaccc gctgtttgag ctgcttcgat agcggcttcg attcggtcgg aatcctcgga
     4741 acccccaaag gaatccacct cgaaaataga tccgaagtcc gcgtcctgaa gaatggaaat
     4801 tcgataactt gcagacctct tcccaggggt tgtcccgcga agggcccgaa ccgcgtactc
     4861 atgaacccca ggcgtaacct tcatgtccaa gtaacaggta tcgtcccacg catcctgctc
     4921 gatcttcagg cttgcaatca cgttcccgtc gcgaaggact tcatagcctg tcgcgctgga
     4981 atcattgcgg tcccaggtga tgagagcata cctgctttcc gcttgtgtgg gtccttgggc
     5041 agccacgacg tgcccgggcg agcgaggccc ttggtttggg ttttcatcgc ccacactgcc
     5101 gaaaccgggg gcggaagaat gggcacaggg tatctgcggt agatgttgct cttccgtacc
     5161 ccccgcaccc cccgtacccc ccgcacccgc cacaccagcc tctccagtgc ctcccgttcc
     5221 cgaaaagcca gcttcccctg catcccccgt ttcatgccat gcatctcccc catcgttttg
     5281 cggcggcttc tgtgggcccg caccccccgc ccctgaaaca cccgcgtctg cgttgccccc
     5341 cgcggcgttg gccccgtctc ctgttcggtc actcgagcaa gcaaggttca acaagcttgc
     5401 cgctatccac gcgaagatga cagcattgcc gcgcttggct tctcgtctcg ttatctcgtt
     5461 gacttgcgcg aggttccgca attgtacgtc cacatccctt ctcatgacat tctccgcgag
     5521 aggatgactg cgcctttctg acccatcatc gaagcatggt ggcatagttc gtttcgaacg
     5581 gctacaaggg ctggcttctc ggtcataagc agcgtgagga ctgccaggga aaggtagata
     5641 ttcgcttcgt tttggagcgg gaacagcgag gatgagcccc cagggccccg acttgccccg
     5701 aaatggagtg ataaaaatct atgtaatatc aatagcttat aagaatgaag gcaccagcag
     5761 tgctatccag cgctttggct ctttcgcagg ggcgttcgag cggaagccaa tgtcgcatga
     5821 acgccaacgt gcgattcctc gtcgtgctcg cagacctcga aacgtgaact acgtcctagg
     5881 ctaggttccg ccgttcgcct cgatggtcct catggctcgc agcctggccc tggcgcgcag
     5941 cagatgtcac cattaggaca aggcgtgtag atggggtccc agcccaaagg acacggttcc
     6001 catgccgtct ccgtaatggg atagcaagcg cctccgctgc tcacacagtc accaacgggt
     6061 ggtacggtaa cgcaacacca tggcgaggcc cccacgcctt cggtcgtgca tcccctcgtg
     6121 gtatccggat aaggagccgg aacgagcagt tgtccggtgg tggacgggca ttgcacgcat
     6181 gcgttggggg ttacggcaac ggcaatgcag acgcctcctt cagattcgca ttgggagagg
     6241 cttccccctg agccaccggt cggcatccca ccgctgcctc ccatggcgcc attgccgcca
     6301 atcccacccg tgccaccggt tggcatcccg ccgctgcctc ccgtggcgcc actgccgcca
     6361 atcccacccg agccaccaga aggagcgcct ccgctgcctg ctttcccacc cgtgccgcct
     6421 ttgcccgctt gccccccttc tccgtcggaa ccggacgttt ccgcgatacc accacaaccc
     6481 aacccgacaa aaacgataag aagcgccttt acagtggttc tgacttcttt catttcacgg
     6541 tctccttgac cttcctccaa gataccgcat gcgctccttg gattcctgaa attacatttc
     6601 tctggggtac cgtttccatc gacgcaatcg agatcgtagc ggccatcgta tccagttctt
     6661 atccgttttt caaagggtcc gtgatcgcaa cacgctgccg ccgccagcgc catcacccgc
     6721 cccgatacgc cccgtgatct cgtcgacgct ggctcaccca ttgctctgcc gtgctcgagg
     6781 aaaccacctc gtacaacacc gcttgtttcc cctcggcacg acgcaaaatg cgacctagcc
     6841 gctgcacgtg ttcccgtacg gacccacttc ccgacaatac gaccgcgaca ttggcttccg
     6901 gaacatccac cccttcgttg agcactttgg acgtcaccac ggcgcaatac tcgccctgcc
     6961 gcaaaccatc caagatcgcg gcgcgctctt tgggtttggt ccgatgggtg atgatcggaa
     7021 ccaaaaaacg acgggaaatc gcgtaggctg tcgcgttgtc ctgcgtgaaa atcaacatcc
     7081 gttcctgccg atgttggtgt agcaacccct cgagcacacg aagcttgccc acggctcctt
     7141 gcgcaagctc tttttgacga cgataggctt gaaatgcttg tcttccttcc gcgcagcgcg
     7201 acgattcgat caagaactga cgccatccat cggaagcgct gaaggagata tttcgcgagc
     7261 gcacaaacga cgtatagact tttcgtgccc gctcgtattc gtctcgctcc gccgcgtcta
     7321 actccacctg taaggtgatg gtttcataat cagccaggta actacctgat aattcaggga
     7381 tttctatgcg gtagacgacg gggccgaggt agtcgtcgag cagcgcatgt cgcccatccg
     7441 ctcgttcgag agtcgccgtc aggcctaatc ggaaaggggc aatcgacgtc cgagcgatgt
     7501 agatatagga ttctccaggt agatgatgct tcccagaaag gtgacgtggc ggggcaggtg
     7561 c
//
LOCUS       DAOSOR010000262         7531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9779_length_7531_cov_4.707865, whole genome shotgun sequence.
ACCESSION   DAOSOR010000262 DAOSOR010000000
VERSION     DAOSOR010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7531
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9779_length_7531_cov_4.707865"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2000)
                     /locus_tag="PK710_14185"
     CDS             complement(<1..2000)
                     /locus_tag="PK710_14185"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOT10923.1"
                     /translation="MALGPDRGSATVGSSKSSTPARSASFDRTAEKVIAIVAEKTGYP
                     TDMLALDLDLEADLGIDTVKQAEIFAELRSVFGLPRRDDLKLRDYPTLEHVIGYVREN
                     IGEESTAVAPSSDGAVSPSAPPALDPVAEKVIAIVAEKTGYPTDMLALDLDLEADLGI
                     DTVKQAEVFAELRAAFELPRRDDLKLRDYPTLGHVIGYVRQGRPDLTPAIPMAPSAPA
                     SAQTPIAREADDLSKQVLEIVAEKTGYPTDMLEWELDLEADLGIDTVKQAEIFADVRE
                     RFQLPRSSDLKLRDYPTLGHVVRFVRDNLSRSAPMAGADPVVSRESDAEVSTSTASLR
                     RLVPTPMVRLPLSACKPTTAMIRAEQRVIVVGDLAGIATKLVELLVKKRVKVLHVDNA
                     ISSEALVKAVTDWAGGQPVDGVFFLPALDRQMSLDEMSVDAFLGVMDRHVRGMYGLAR
                     ALYDSFDRGGTYFMTATNLGGTFGYGATAAKDPCAGPISGFTKAFARERDKAVVKVVD
                     FDPTESKETIARRLLEEVERDPGAVEVGYQSSLRYGITLVEQQCPDTINEEYLTSESI
                     VLVTGSGGAITSAIVADLASTSHATFHLLDLPPAPRMDSPDLDRVSTDREGLKREIMD
                     RLRAGSRERVTPVMIERELARIEREAAARDGIRAVEKAGGKAV"
     gene            complement(2001..7148)
                     /locus_tag="PK710_14190"
     CDS             complement(2001..7148)
                     /locus_tag="PK710_14190"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOT10924.1"
                     /translation="MSYEALETAERAVAIIGVGAVLPDAPNAKKFWENVVSARYSITD
                     VPEDRWSSAEYYDPDPKAVGKTYSKIGGWVRDFSFDTLKYRIPPRVAAAMDMAQMWGI
                     AAAHEALTDAGHPDRPLDTDRTAVIVGNAMGGQLHYETTFGVVAPPSISRVLRVSKTG
                     KGLDSSTYSALERELVDGLRGLVPGITEDSMPGELANVVAGRIANAFDLHGPNYVCDA
                     ACASSLAALDAAIEGLLARHYDAVVCGGVDRNMGPATFVKFCKVGALSPDGSRPFAKG
                     ANGFVMGEGAAMFVLKRLADAERAGDRIYAVIRAVGGASDGKGKGITAPNPEGQKRAV
                     LRAYERAGFALSTVTQIEAHGTSTIVGDVAEVAAINELYRGVDLPKRSVALGSIKSQI
                     GHLKAAAGAAGLLKGVLAIHHKTLAPSAQFVEPNPNIRFEDGPFFVNTQARPWERPAQ
                     GLRRLGVSAFGFGGTNFHVCLEEHVPEMLTRRGLQVQVPGSAERTTASSMARSSQEHA
                     EPPPMLRGLLMAHGNTLGEVRSRLVEIAQEASKGITPPYELPSAEAMTAPERIAIDFG
                     SAKELETSVAKAIECIDDDRPAAWRLARGKSVFRGYEAPGKIAFLFPGQGSQYVNMLR
                     ELRDISPTVRRIFDEADDVMRDRLDGRTLTQLIFVDSTDKELLANAEEALRHTTVTQP
                     AMLAADNALASLLQDFGIFPDMVIGHSLGEYAALVAAGVMPFADALVAVSARAREMAN
                     VSLADSGKMAGVIGQYDKIPPVLEKVGGYVVAANRNSYKQTVIAGASDAVDKASDMLR
                     DAGLRVIPLNVSHAFHSNIVAPASEPLRRVIQGLRVSPPRLPVVANVDGKFYPMDPAC
                     REAIIDRLARQIASPVEFVDGLETLYESGVRVFVEVGPKKVLASFAEEVLSERGDIVA
                     LATNNPKKGPEASFGAALAYLYSIGRGNPRLEANTPIAAPVELTTRVEKPREPIASEP
                     PPSAPMTVPMAPPSGSPSLDTRTTLELGRIFADFLDRGMRVYRNGQSEGAREASPGVT
                     PHAQGSVVITGASVGLPGVEHRLFDDEGVERILRGDQLIGPIPKPLQDLMVDKNVVRL
                     IKSETEGPRLDLITENSDVLHLAGRRGDFDLVKDFGVAEERADAYDITTAMAIAAGLE
                     ALRDAGIPLVRRYRKTSKGTFLPDRWMLPEPLADETGVVFASAFPGYNRFAELMQEYQ
                     EYRAARARVEMLERVRMHMLRTDTAAIDAMIAEERGKLEANPYRFERKFLFEILAMGH
                     SQFAELIGARGPNTQVNSACASTTLAIATAEDWIRSGRCSRVIVIAADDVTSDSMIDW
                     IGAGFLATGAATTEKDVTQAALPFDRRRNGMILGMGACALVVESHDAVRARGMQGIAE
                     ILASDIANSAFHGTRLDVNHICSVLDRVVATAESRHGIDRRQIASHTVFISHETYTPA
                     RGGSASAEVHALRTAFGDAASDIVIANTKGFTGHPMGVGIEDALAVKVLESRVVPPIP
                     NFREVDPELGTLNLSRGGSYPVDYALRFSAGFGSQLAITLYRRVAGKHERLVDASRYK
                     DWLRVMSGYPDADTEVDHRTLRVRDQGVPSELAQSTGWKYGQVPINRVMAAETSASAT
                     TVAPSYPPAGRHDPISVAPPSMSTDAAAPAERSDLSARVLAIVAEKTGYPTDMLALDL
                     DLEADLGIDTVKQAEIFAELRSVFGLPRRDDLKLRDYPTLERVIEYV"
     gene            complement(7175..>7531)
                     /locus_tag="PK710_14195"
     CDS             complement(7175..>7531)
                     /locus_tag="PK710_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10925.1"
                     /translation="REARLTRHQHVLFRMGELATRAETAAVMCRWALMDDHDPTRGDA
                     SALRAMARIYARETLTAIHQQAMGWLRASDAVSASQSAAIAAQMGIDALYKGYGGWLD
                     DMNHVARVTLGMEASA"
BASE COUNT         1589 a         2400 c         2039 g         1503 t
ORIGIN      
        1 accgcttttc cacccgcctt ttccacggcg cggatgccgt cgcgcgccgc cgcctcgcgc
       61 tcgatgcggg caagctctct ttcgatcatg accggcgtga cccgctctcg agaaccggcc
      121 cgaagcctat ccatgatttc gcgcttgagt ccctcccgat cggtcgagac tctatccaaa
      181 tccggtgagt ccatccttgg agctggagga agatccaaca gatggaacgt agcatgggag
      241 gtcgaagcca aatcagcgac aatagctgaa gtaattgctc ctccagagcc ggtgaccaat
      301 acgatgctct cggacgtaag gtattcctca ttaatcgtat cgggacactg ttgttccacc
      361 agcgtgattc cgtatcgaag gctactctgg tatccaactt caactgctcc gggatcccgt
      421 tcgacttcct ccagcaggcg cctggcaatg gtttcctttg attcggtcgg atcgaaatcg
      481 accaccttga cgaccgcctt gtctcgttcg cgagcaaaag ctttcgtgaa tccactgatg
      541 ggtcccgcac aaggatcctt ggccgccgtc gctccgtatc caaacgtgcc cccaagattg
      601 gtggccgtca tgaagtacgt ccccccacga tcgaacgagt cgtaaagagc gcgcgcaagc
      661 ccatacatcc ctcgcacatg gcgatccatc acgcccaaaa acgcatccac gctcatctcg
      721 tcgagagaca tttgacgatc gagcgccggc aggaaaaaca cgccatcgac aggctggccg
      781 cctgcccaat ccgtcaccgc cttgaccagc gcctccgacg agatggcatt gtcgacgtga
      841 agcaccttga ctcgcttttt gacgagcaac tccacgagtt tcgtcgcgat tcccgccagg
      901 tcccccacca cgatgactcg ctgctccgct cgaatcatcg ctgtcgttgg cttgcatgcc
      961 gaaagaggca aacgaaccat cggtgtcggc acgagtcgac gcaacgaagc ggtcgacgtc
     1021 gatacttccg cgtccgattc gcgcgacacc accggatccg cacccgccat tggcgcggac
     1081 cgagacaagt tatcgcgaac gaatctcacg acatgcccga gcgtcggata gtctcgcaac
     1141 ttcaaatcgc tcgagcgagg aagttgaaag cgctcccgca cgtcggcaaa gatctcggct
     1201 tgcttgaccg tatcaatccc caagtccgcc tcgagatcca gttcccattc gagcatatcc
     1261 gtcggatatc ccgtcttttc cgctacaatc tcaagaactt gcttcgataa atcgtcggct
     1321 tctcgggcga tgggcgtttg tgccgaggcg ggcgctgacg gtgccatggg tatcgccgga
     1381 gtcaaatccg gacgcccttg gcgaacgtat ccgatgacgt ggccgagcgt tggatagtcg
     1441 cgaagtttca agtcatctcg acggggaagc tcgaaagccg cgcgaagctc ggcaaatacc
     1501 tcggcttgct tgaccgtatc gatacccagg tctgcttcca gatcgagatc gagggccagc
     1561 atatcggtcg gataccctgt cttttccgcc acgatcgcga tcaccttttc ggcgaccgga
     1621 tcgagcgcgg gtggagcgga tggagacacc gcgccgtcgg aggaaggcgc cacagccgtc
     1681 gactcttcgc cgatgttttc acgaacgtat ccaatgacat gctcgagggt cggatagtcg
     1741 cgcagcttca aatcatcgcg acgaggcaag ccgaataccg atcgcagctc cgcgaaaatt
     1801 tcggcttgtt tgactgtatc gatacccaga tccgcttcca gatcgagatc gagagccagc
     1861 atatctgtcg gatatcctgt cttttccgcc acgatcgcga tcaccttttc ggcggtacgg
     1921 tcgaagcttg cggagcgggc aggagtcgag gatttcgaag aacccaccgt tgccgaaccc
     1981 ctgtccggcc ccaaagccat tcaaacatat tcgattactc gctcgagcgt cgggtagtcc
     2041 cgtagcttca aatcatcgcg acgaggcaac ccaaataccg atcggagttc ggcgaaaatt
     2101 tcggcttgtt tgacggtatc gatgcccagg tccgcttcca gatcgagatc gagggccagc
     2161 atatccgtag gatatccagt cttttccgcc acaatcgcga gcacccgggc actcagatcg
     2221 cttcgttcgg caggcgccgc ggcatccgtg gacatcgacg gcggagcgac cgaaatggga
     2281 tcgtggcgac cggcgggggg gtacgacggc gcaaccgtcg tggcgcttgc cgatgtctct
     2341 gcggccatca cgcgattgat gggcacctgc ccgtatttcc atccggtgct ctgggcaagt
     2401 tccgaaggga ctccttgatc tcgaacccgc agggttcggt gatccacctc agtatccgca
     2461 tcgggatatc cggacatgac gcgaagccaa tccttgtagc gggaggcatc gactagccgt
     2521 tcgtgcttac ccgctactct tcgatatagg gtaatggcga gttgcgaacc aaatcctgcc
     2581 gaaaaacgaa gcgcataatc cacggggtag ctcccgcctc gtgacaagtt gagcgtaccc
     2641 aactccggat cgacttctcg gaagttggga atagggggaa ccacacggct ctccagcacc
     2701 ttgaccgcta gggcgtcttc gatgcccaca cccatcggat ggcccgtaaa ccccttggtg
     2761 ttggcaatga cgatatcgga agcggcatca ccaaaagccg tgcgaagcgc atgcacttcc
     2821 gccgaagcgc taccgccccg cgctggcgtg tacgtttcat gggagatgaa cacggtatgg
     2881 gaagcaatct ggcgacggtc gatgccgtgc cgggattcgg ccgtggcgac cacccggtcc
     2941 aacaccgaac agatatggtt cacgtcgagg cgtgttccgt gaaacgcgct attcgcaatg
     3001 tccgaagcaa gaatctcggc gattccctgc atgcctcgtg cccgaacggc gtcgtgggac
     3061 tccaccacca aggcgcaggc tcccatgccc aaaatcatgc cattgcgacg acgatcgaac
     3121 ggcagcgcgg cttgcgttac atccttctcc gtcgtggcgg cgcccgtggc caaaaatccc
     3181 gcgccaatcc aatcgatcat gctgtcgctg gtgacatcgt cagccgcaat gacaatgacc
     3241 cgggagcagc gaccacttcg gatccaatcc tcggccgtgg caatcgcgag agtcgtcgac
     3301 gcacaagcgg agttgacctg ggtattggga ccacgcgcgc caatgagttc cgcgaactgc
     3361 gaatggccca tcgcaaggat ttcaaaaaga aatttccgtt cgaatcgata gggattggcc
     3421 tcgagcttgc cgcgctcttc ggcaatcatc gcgtcaatgg cggccgtatc cgtccgcagc
     3481 atgtgcatgc gtacacgctc gagcatctcg acccgcgcgc gggcggctcg atactcttgg
     3541 tattcctgca tgagttccgc aaagcggttg tatccaggga atgcggaggc aaacaccaca
     3601 cccgtttcat ccgcgagagg ttcagggagc atccaccgat ccggaaggaa cgtccccttc
     3661 gatgtcttgc ggtatcgtcg caccagcgga ataccagcgt ctcgcaacgc ttccaaccct
     3721 gccgcaatcg ccatcgcggt cgtgatgtcg tacgcgtcgg ctcgctcttc ggcgacgcca
     3781 aagtctttca ccaggtcaaa gtcgccgcga cgccctgcca aatgaagtac atcggagttc
     3841 tccgtgatga gatcgagacg cgggccttcc gtctcgcttt tgatcaaacg aaccacgttt
     3901 ttatcgacca taaggtcttg taagggctta ggaatcggtc cgatgagctg gtctccacgt
     3961 aggattcgct cgactccctc atcatcgaac aggcgatgtt ctactcccgg caggcccaca
     4021 ctcgcccccg taatgacgac cgatccttgg gcatggggtg ttacgccggg gctcgcttct
     4081 cgcgcgccct cggactgtcc atttcggtat acacgcattc cgcgatcgag aaagtccgcg
     4141 aaaatacgtc ccaattccag ggtcgtccgc gtgtccagag aaggggaacc cgaaggaggg
     4201 gccatgggaa ccgtcatcgg agcggacggt ggaggttccg acgcgatcgg ctcgcgcggc
     4261 ttttccaccc tggtcgtgag ttccaccggc gcagcaatgg gagtattcgc ttcaagacgc
     4321 ggatttccac gaccaatcga atataggtag gcgagagccg caccgaagga agcctcgggt
     4381 ccctttttgg gattgttggt ggccagagcc acgatgtctc ctcgctccga aagaacctct
     4441 tccgcgaacg aagcgagcac ttttttggga ccgacctcca caaacacacg cacaccagat
     4501 tcgtatagcg tctcgagacc atccacgaat tcaacgggcg atgcgatttg acgagccaat
     4561 cgatcgatga tggcttcacg acaggccgga tccatcggat agaacttgcc atcgacgttg
     4621 gccacgaccg gaaggcgggg aggagagacc cgcaagcctt gaatgacacg acggagcggc
     4681 tcactggccg gggccacgat attggaatga aatgcgtgag aaacgttgag cggaatcaca
     4741 cgaagaccgg catcgcgaag catgtccgac gccttgtcca ccgcatcgct ggctcccgcg
     4801 atgaccgttt gtttgtacga gttacgattg gccgcgacca catatccacc caccttctcg
     4861 aggacgggag gaatcttgtc atattggccg atgactccgg ccatttttcc ggagtccgcc
     4921 agcgatacgt tggccatctc cctcgcacgc gcactcaccg ccaccagcgc atcggcaaat
     4981 ggcatgactc ccgccgctac caaagcggcg tattcaccca agctatgacc gatcaccatg
     5041 tcgggaaaaa tcccaaagtc ctgcagcagg ctcgccaagg cattatctgc cgcaagcatg
     5101 gccggctggg tcaccgtggt gtgacgcaac gcctcctcgg cgttcgccaa tagctcctta
     5161 tccgtggaat ccacaaaaat gagctgcgtc agggtgcggc catcgagccg atcacgcatc
     5221 acatcgtcag cctcatcgaa aattcgacga accgtggggg aaatatcccg cagttcgcga
     5281 agcatattga catattgaga gccttgacct gggaagagaa aggctatctt tccaggggct
     5341 tcgtagccgc ggaacaccga ttttccacgg gccaaccgcc atgccgccgg acgatcatca
     5401 tcgatgcact cgatggcctt cgccacgctc gtttcgagtt ctttcgcgga gccaaagtca
     5461 atcgcgatgc gctcgggcgc agtcatcgct tccgcggaag gaagctcata cggcggtgtg
     5521 atccccttgc tggcctcttg cgcaatctcc acgagacgtg atcgcacttc gccaagcgtg
     5581 tttccgtgag ccatgagaag cccacgcagc atcggcggcg gctcggcatg ctcttgcgac
     5641 gatcgagcca tgcttgacgc ggtggttcgc tccgcgcttc caggcacctg cacttgcaaa
     5701 ccgcgtcgag tcagcatctc cggcacatgt tcttcgagac agacatggaa gttggttccc
     5761 ccaaatccaa acgcactcac gcccaatcgt cgaagccctt gggcaggacg ttcccacggt
     5821 cgcgcttggg tattgacgaa gaagggcccg tcttcgaagc ggatattcgg gtttggctcc
     5881 acgaactgcg cggagggcgc cagggtttta tgatggatgg ccaacacgcc tttgagaaga
     5941 cccgcggccc ccgccgcggc tttcaagtga ccaatctgcg acttgatgga accaagcgcc
     6001 accgaacgct tcggtaagtc aactccgcga tacagctcat tgatggccgc gacctccgcc
     6061 acatccccca cgatggtcga tgttccatgc gcttcgattt gtgtcaccgt agacagagca
     6121 aatcccgcac gttcgtacgc ccgaagcacg gcgcgttttt gcccttccgg attgggagcc
     6181 gtgatgcctt tgcctttgcc atcacttgct cctcctaccg cgcgaatcac cgcgtagatt
     6241 cgatctccag cccgttccgc atcggccagc cgcttgagca cgaacatcgc cgcgccttcg
     6301 cccatcacga aaccattggc gcctttggcg aacggtcgcg acccatccgg agacaaagcc
     6361 ccgaccttgc aaaacttgac gaaggttgcc ggccccatgt tccgatcgac acctccacaa
     6421 acgaccgcat cgtaatggcg cgccaatagt ccttcgatcg ctgcatccag cgccgcgagt
     6481 gacgacgcac aggcagcatc acaaacataa tttggaccgt gcaaatcgaa ggcgtttgcg
     6541 atgcgtcccg ccaccacgtt ggccaactcc ccgggcatcg aatcctcggt gataccggga
     6601 acgagaccac gcaatccgtc gaccaactcc cgctcgagcg ccgaatatgt cgaggaatca
     6661 aggccctttc ccgttttcga caccctcagc acccgcgata tcgaaggcgg agccaccaca
     6721 ccaaaggtgg tctcgtaatg aagctgccca cccatcgcat tacccacgat gacggctgtc
     6781 cgatccgtat cgagggggcg atcgggatgc ccggcatccg tgagcgcttc atgcgcagcc
     6841 gcaatccccc acatctgcgc catatccatg gccgcagcca cacgcggagg aattcgatac
     6901 ttgagtgtat cgaacgaaaa gtcgcgtacc catccaccga tcttcgagta cgtcttaccc
     6961 acggcctttg gatctggatc gtaatactcc gcggaagacc aacgatcttc cggcacatcg
     7021 gtaatgctgt agcgtgcgga gacgacgttt tcccaaaatt tcttggcatt cggcgcatca
     7081 ggaagcacgg ctcccacacc gatgattgcc actgcccgct ccgccgtttc gagtgcttcg
     7141 tagctcattg ctctattaac tcctcgtcgt ttcctcaggc gctcgcttcc attcccagcg
     7201 tgacccgagc gacgtggttc atgtcgtcaa gccaaccacc atagcccttg tacagagcat
     7261 cgattcccat ttgcgcggca atcgccgcac tttgcgatgc gctcaccgca tccgaggcgc
     7321 gcaaccatcc catcgcttgt tgatggatgg ccgtgagtgt ttcgcgcgcg tagatccgag
     7381 ccatggctcg aagcgccgag gcatcccccc gcgtcggatc atgatcatcc atcaacgccc
     7441 agcgacacat caccgccgcc gtttccgctc gagtcgccag ctcgcccata cgaaacagca
     7501 cgtgctgatg ccgagtcagc cgcgcctctc g
//
LOCUS       DAOSOR010000263         7516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9803_length_7516_cov_4.287093, whole genome shotgun sequence.
ACCESSION   DAOSOR010000263 DAOSOR010000000
VERSION     DAOSOR010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7516
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9803_length_7516_cov_4.287093"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(255..1391)
                     /locus_tag="PK710_14200"
     CDS             complement(255..1391)
                     /locus_tag="PK710_14200"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT10926.1"
                     /translation="MKSSRAWMVVLLTVGASGCQQDSHPACSLSETWQAFAAFHQHCV
                     DEANKTPPKPVEPPWTEQDDALPDTLEGQREEILRKLRERMGVSEEAITKLRDAFGTY
                     RTGQGNPKVTKHPMTRAECRAIRESAETFPASEVCGEKNMVALFNPSKHETEKDATVC
                     IDQFEFPNIACEYPMTWVQAQQAQRICKALGKRLCDAHEWEGACYGELRSPEEEYAFT
                     LDRENMSGLHNFNNKKNGWIRWAYGEQKNHKLCATTSRKSDKCVASEWEYCGSNTFPA
                     GAFPKCVSRFGVYDQHGNAAEHMSLPMKPEQLGSRGGIGRTEMKGSWFIFDFYEAHID
                     DCRWRAPDWHGSEVMHHNSHANYHLGFRCCKDIAPSPSTQTTKD"
     gene            complement(1451..3097)
                     /locus_tag="PK710_14205"
     CDS             complement(1451..3097)
                     /locus_tag="PK710_14205"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOT10927.1"
                     /translation="MKLSLHIDNDLVRGLWMAGIFAVAGAIIGLLALPPGPRRIDPDA
                     PGPNFTLLGQRIPSSADADERALQLVRRYANANITVKIPGGGEHLIARSALGAQVQRS
                     RLMALLEQMRDPASPLRQHASTQDTEGIALPVPLALSDERALATLLELKDHVDRPAID
                     ARLDLESRKLLPEQYGIQLEVYGTLARLHHAARTGQDEIEAVVSQLEPRLRSEQLGNV
                     TFENVLGYFETAYARGAKHELRTYNLRLAASRLDGHVILPGEVFDFNEVVGPRDEAHG
                     YKIAPVIAEGELVDGIGGGTCQISGTLHAAAYFAGLEIVARRAHTRPSSYIKMGLDAA
                     VSYPTITLKLKNNFSFPVVLHETVRDGVVRAEILGPKRDLTVTFVRKIMSVQAYQEVE
                     RNDPKLPAGVRKLSQRGVPGFKVQKHRILRDGPFAVRERSQDTYPPTTQIVRVGTGDM
                     PREAVDVQDDPHPEYTADEYLVITQGPGILTRGVDRDTPGGPMVENRTPGKTGRAGWM
                     KESGFPVFKAEPAQESNEDGDGKKDRKRDKKGEKKAEKKG"
     gene            complement(3414..3911)
                     /locus_tag="PK710_14210"
     CDS             complement(3414..3911)
                     /locus_tag="PK710_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10928.1"
                     /translation="MKTIPLLVGLITLGLLSACSDDDADQSMGQEHNGLYQITKYQSK
                     DGCSEQQPWVDQAPKSQYFKLNAASLMGTRILEWHWCSGPSENTCDEAIDLVGSFVWD
                     QGQWKSSITWAMGSAPDCSLGSTQGIPVFSSTGMELTIRTSEGQVQLQGSEKCKTELV
                     DKYRD"
     gene            4219..5910
                     /locus_tag="PK710_14215"
     CDS             4219..5910
                     /locus_tag="PK710_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10929.1"
                     /translation="MASESEPQGEARPRIPRSMLAVLLTSVLAILGHLPSITNGFVID
                     DFPLLVHNPHVRTLRGLFVILRSDLFVASAEPRAFSYYRPLSGLSYWVTWQAFGDNPT
                     LHHVFNLGLHAVVVGCFTHFMIRSGLRIYTSMLAASLFAIHPATADIVAYLGGRQDLL
                     GWVTIFVSAILILRCKNFWAQASVAFFGSLCAGFFREYFWIAPLLFFGIFELISGERQ
                     ASSDSSTKEARRGNKQRAWVLVAGILAVLVSLGIRNVLGIGQLVNEWNDSTMVMGCVG
                     STALRLIHNTVAPTDLALNVSCTPLADTTAWLLLGGGALVLLAPVVWLAKTNARTKAV
                     LACAGVFIMASGAVIHGFHGARYGVISDRYTYSFLVGALLFAASLTEHVHQRFRLPAR
                     AKPSLLRPLVPWILLATPLSLLPLTWARDQDWKSDTTLQIAMWRDRPHDPESQYAEAL
                     RLIDQGDYEAAYPLCVAYRNAAPQAERASLCIAGYLYLKGRAEEAVPLACHFARTRPG
                     NVQGRHLCLSCLLATGRAEEATALISDWKAMFGESSEMIEAQEVLQRMKQQAPAP"
     gene            6039..>7516
                     /gene="uvrC"
                     /locus_tag="PK710_14220"
     CDS             6039..>7516
                     /gene="uvrC"
                     /locus_tag="PK710_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOT10930.1"
                     /translation="MIPETLVSKLESLPSKPGCYLFKNAEGTVIYVGKATSLRSRVRS
                     YFQQRSTDSRAQLPFLIRSVVDVETIITASEKEATILEDSLIKQHKPKYNVKLRDDKS
                     YLSLRLDERHEYPRLELVRRPSADGARYFGPHHSATAARRTLHFINKHFHLRTCSDAE
                     LVSRKRPCLQYHIKRCPAPCVIDIDREHYASNVSAVMLFLEGRHDEVSAQVEQRMREA
                     SRRMDFELAAVYRDQLRAVQTVRETQRVVAIKDVDQDVIGLHRDADLCELALLFIRKG
                     RVVDTATIPMHKVEVGDEEVIGAFLLHYYGEQGPAAFAIPDEVIVPVLPESPKGVQEW
                     LTERRGRKVQLLHPQRGPRSQLLALAMDNAAHAFAEKRRAKDDVQERLLDLQRRLRLP
                     TIPRVVECLDISHHAGKDTSGGLVRMVDGQFDTTGYRSYHVHHAAEGDDYAAIYDVLS
                     RRFRRAMQQEQERSDEESRHDDPSKRMPWESPDLLVVDGGRG"
BASE COUNT         1527 a         2139 c         2068 g         1782 t
ORIGIN      
        1 gtgcgtggag gggcgctcaa cagcgagcag gatgagcttc gctgtgacta cgacttggcg
       61 ttcaacccgt tcgacacatc tccaagtatt ggttttcgtt gctgttttcc gtaaaaaggg
      121 cgatagggag acataagcgg tagaccacga ttcctcaccg aatcccacga gggcaatgct
      181 tcgacgaatg ctcgcacctt gcgagttgct cacgctcgtt ggcggtgaaa cgtcggcttc
      241 ggcagggcaa tcgttcagtc tttggtggtt tgggtcgagg ggctcggagc aatatcttta
      301 cagcatcgga atcccaagtg gtagttggcg tggctgttgt ggtgcatgac ctcgctgccg
      361 tgccaatcag gcgcgcgcca acggcaatca tcgatatggg cctcataaaa atcgaaaatg
      421 aaccagcttc ctttcatttc cgtgcggcca atccctcctc ggcttcctag ctgttcgggc
      481 ttcatcggta ggctcatatg ctcggccgca ttgccatgct gatcgtacac tccaaaccgg
      541 ctcacgcact tggggaatgc gccagcggga aacgtattgg atccgcaata ttcccattcg
      601 ctcgcgacgc atttgtcgga cttacggctc gtcgtggcac atagtttgtg atttttttgt
      661 tcgccgtacg cccagcgaat ccatccgttt ttcttgttgt tgaagttgtg gagcccggac
      721 atgttttcgc ggtcgagtgt gaatgcgtat tcttcttcgg ggctcctcag ttcgccataa
      781 caggcgcctt cccattcatg agcatcgcat agccttttgc cgagagcttt gcagatacgt
      841 tgtgcttgct gcgcctggac ccatgtcatc ggatactcac aagcgatgtt tgggaattcg
      901 aattgatcga tgcacacggt cgcatctttt tctgtctcgt gtttcgatgg attgaacaac
      961 gcgaccatgt ttttctcacc gcaaacctca ctggcgggaa acgtctcggc gctttcgcgg
     1021 atggcgcggc attcggcgcg tgtcatcgga tgcttcgtga ctttgggatt gccttggccg
     1081 gttcgatagg tgccaaatgc gtcacgaagc ttggtgatgg cttcttccga tacgcccatt
     1141 cgctctcgga gctttcgaag aatctcctcg cgttgcccct cgagcgtatc gggcaaagca
     1201 tcatcttgtt cggtccaagg aggttcgaca ggtttgggcg gtgttttgtt ggcctcatcg
     1261 acacaatgct gatggaacgc agcaaaggcc tgccacgttt cggatagcga acaggcgggg
     1321 tgcgaatctt gttggcaacc gctcgcaccc acggtcagaa ggacgaccat ccaggcacgc
     1381 gaagatttca tgggaaccgc ttcgtagcat tgcgaaagaa gggggcaaaa aaggttgcga
     1441 tcatcgcttg ttatcctttt ttctccgcct ttttttctcc cttcttatcc cgtttccgat
     1501 ccttttttcc atcgccatct tcgtttgatt cttgcgccgg ctcggctttg aacacaggaa
     1561 agccagattc tttcatccat cccgcgcgcc ccgttttccc gggagttcga ttctccacca
     1621 tcggcccccc cggcgtgtcc ctatccactc ctcgcgtcaa tatcccaggg ccttgtgtga
     1681 tcacgaggta ttcgtcggcg gtatattcgg ggtgaggatc atcttgtaca tcgacagcct
     1741 cgcgcggcat gtctccagtc ccaacgcgca cgatttgggt ggtgggcggg taggtatcct
     1801 gggagcgttc gcgaacggca aaggggccat cacgcaagat gcgatgcttt tgcactttga
     1861 atccgggcac gcctcgctgg gatagttttc gaacgccggc gggcagcttc ggatcgttgc
     1921 gttcgacttc ttgataagct tgcacgctca tgatctttcg cacgaaagtg acggtgagat
     1981 cacgcttggg accgagaatt tcggcgcgaa ccacgccatc ccgaacggtt tcgtgcagca
     2041 cgacagggaa gctgaagttg tttttcaatt tgagggtgat ggttggatac gaaaccgcgg
     2101 cgtcgagacc catcttgatg taggaactgg gacgggtatg ggcgcgacga gccacgattt
     2161 ctagaccggc aaaataagct gccgcatgca gtgttcccga gatctggcaa gtcccgccac
     2221 caataccatc caccaattcc ccctcggcaa tgaccggcgc aattttgtac ccatgagcct
     2281 cgtcgcgagg tcccaccacc tcgttgaaat caaatacctc gccgggcaaa atcacatgac
     2341 catcgagacg cgaagccgca agccgtaaat tatacgtccg aagctcgtgt ttggcgcccc
     2401 tcgcatacgc cgtttcgaag taccctaaca cgttttcgaa agtgacattg ccaagctgct
     2461 ccgaacgaag tcgaggttcg agctggctca ccaccgcctc gatctcgtcc tgtcccgtac
     2521 gcgccgcatg gtgcaagcgt gcaagcgttc catacacttc gagctggatc ccgtattgct
     2581 ccggaaggag tttgcgactt tccagatcga gacgtgcatc gatcgctggc cggtccacgt
     2641 gatccttgag ttcgagcaac gtggcgaggg cccgctcatc gctcaaagca agcggaacgg
     2701 gaagagcaat cccttccgta tcttgggtgg atgcatgttg cctaagagga ctggcgggat
     2761 cgcgcatttg ctcgagcaga gccatgagcc gagagcgttg cacctgcgcg ccaagagccg
     2821 accgcgcaat gaggtgttca ccacctccag gaatcttcac cgtgatgttg gcgttggcgt
     2881 agcgtcgaac caattgcaaa gctcgttcat ccgcatccgc actggaagga attcgttgcc
     2941 cgagcagcgt aaagttgggg ccaggagcat ccggatcgat tcgtcgcggt ccgggcggaa
     3001 gggcgagaag accaatgatg gcaccggcca cggcgaatat cccggccatc cacaaaccgc
     3061 gaacaaggtc attatcaatg tgcagcgaca gtttcatccg ttcttcgaac ctgcgctccg
     3121 atggcgcaaa gctctctgta gcaacctgcc ttatgcgatg ccagagccgt gatctcgaca
     3181 gcggatgcgc ctggacatga ctcatcgcgc ctcggcccgt gaaaaggcga taccttgggc
     3241 cgtgtcctcg cctgccatct ttggctcatg agcgatattt tattagcaaa tacagtaagt
     3301 tacacatcaa ccgaaacaag cgatccgcgc tttgccagcc atcgcatcga cgagccttcc
     3361 atggtgggtc acaagcgatt ggcttcgagc aattccatgc gctcacaggt aagctaatcg
     3421 cggtacttat cgaccaattc ggtcttgcac ttctcgctgc cttgcagttg cacctgccct
     3481 tccgaggtgc ggatggtcaa ttccataccc gtactggaaa agacagggat accctgcgtc
     3541 gaccccagcg agcagtcggg agcggacccc atcgcccagg ttatcgaact cttccattgt
     3601 ccttgatccc aaacaaatga gcccaccaaa tcgatggcct cgtcgcacgt gttttcactt
     3661 ggccccgaac accaatgcca ttcgagaata cgggtcccca tgagggaagc tgcgttgagc
     3721 ttgaagtact gcgacttcgg cgcttgatcc acccacggct gctgttcgct acagccgtct
     3781 ttcgattggt acttggtgat ctgataaagt ccgttgtgtt cctggcccat ggactgatcg
     3841 gcgtcatcat ccgagcaagc gctcagcagt cccagggtta tcaggccaac aagcaaggga
     3901 atggttttca tgatgctctc ctttggcggg cccatcgtcc caatgatctc gaccaacaac
     3961 gaagctgcca tcggccgaca ggagccacta agattccatc ccatggtacg aggcaaatgg
     4021 taagggaaca agatcgcgtc ccgcctgtat agggactgct ggtgaaagta tgcgatgacc
     4081 aacgtgaact cccagcatct ccctgcgcta ccacgacgat gagatctgtt cacaacacgc
     4141 cggtggccgc ggaggggaaa caagtcttga ctctcctttt cattagcttc ggaattcttt
     4201 tggcgcgaag ctctcgtcat ggcttcagag tcggaaccac aaggagaagc tcgacctcgc
     4261 atacccagat cgatgctggc agtcctgttg acctcggttc tggctatcct tgggcacctg
     4321 ccatcgatca cgaacggctt cgtcatcgat gactttccat tgttggtcca caatccccac
     4381 gtcagaactt tgcgaggatt attcgttatc ctacgttcgg atctgttcgt cgcttcagcc
     4441 gaacctcgcg cgttttctta ttatcggccc ctgtcagggc tgtcttattg ggtgacgtgg
     4501 caggctttcg gtgacaaccc cacactgcac cacgtcttca acctgggatt gcacgccgtc
     4561 gtcgtggggt gcttcaccca tttcatgatt cgttcgggcc tgcgcatcta cacatcgatg
     4621 ctcgcggcga gcctgtttgc cattcatcca gcgactgccg atatcgttgc gtaccttggc
     4681 ggacgacaag accttctcgg ttgggtaacc atttttgtca gtgcgatcct gattctccgc
     4741 tgcaaaaact tttgggcgca agcttcggtg gctttcttcg gttcgctatg tgccggtttt
     4801 tttcgcgaat acttctggat agctcctctt cttttctttg gaattttcga gcttatttct
     4861 ggcgaacgac aggcatcgag cgattcgtcg accaaagagg ctcggagggg gaataaacaa
     4921 agagcctggg tcttggtggc tggaatcctg gccgtccttg tatccttggg gattcgaaac
     4981 gttcttggga tcggacagct cgtcaacgaa tggaatgaca gcaccatggt gatgggctgc
     5041 gtggggagta cggcattgcg cctcattcac aacacggtgg cacccaccga tctggctctc
     5101 aacgtctcat gcaccccgct tgctgatacc accgcatggc tgctgctcgg ggggggagcg
     5161 cttgtcctgc tcgcccctgt cgtttggctt gcgaagacca acgctcgcac caaagccgtg
     5221 cttgcttgcg cgggtgtctt catcatggct tccggtgccg ttatccacgg ctttcacggg
     5281 gcgcgctacg gagtcataag tgaccgatat acctactctt ttttagtcgg cgcccttctg
     5341 tttgcagctt cgctcacgga acacgtccat cagcgtttcc gcttgcctgc gcgggcgaaa
     5401 ccatccttgt tgcgccccct tgttccgtgg atactgcttg cgacgcctct atccttgctt
     5461 ccgctgacat gggcgcggga ccaggattgg aagagcgata ccacattaca gattgcgatg
     5521 tggcgagatc gccctcacga ccccgagtcc caatacgccg aggccctgcg actcattgat
     5581 cagggcgatt acgaggctgc ctatccgctc tgcgtagcct atcgcaacgc tgctccccag
     5641 gccgagcgcg ccagtctatg tatcgccggt tatttgtatt tgaagggaag agccgaggaa
     5701 gccgtccccc tggcctgcca ttttgctcga accagaccgg gcaatgtcca ggggcgacat
     5761 ctatgtctct cttgccttct ggccaccggt cgagccgagg aagccacggc gttgatttcc
     5821 gattggaaag ccatgttcgg tgagtcttcc gaaatgatcg aggcgcaaga agtcctgcag
     5881 cgaatgaagc agcaagcccc cgctccctga ttcccctcac gatgccgttg ctgaccccta
     5941 acatgcaaac cacaggtccg tcttgcatcg atccgcgaat cggcgtctgg gcgacgcgaa
     6001 gctctttgta gcaacgtgcg ttgtgctatg ccaaagccgt gatcccggag acactcgttt
     6061 ccaagctcga atccttacct tccaaacccg gttgttacct attcaaaaac gccgaaggaa
     6121 cggtgattta cgtcggaaaa gcgacaagct tgcgctcccg tgttcgttcc tacttccaac
     6181 aacgctccac ggattcacgc gctcaactgc cctttctcat tcgttctgtc gtcgatgtcg
     6241 agacgatcat taccgccagt gagaaggaag cgaccattct cgaagactcg ctcatcaaac
     6301 aacacaagcc aaagtacaat gtcaaacttc gcgatgacaa aagctacttg tcgttgcggc
     6361 tggatgagcg gcacgagtac ccacgcctag agttggttcg tcgtccatcc gccgatggag
     6421 cgcgatactt cggcccgcat cattcggcga cggcagcgcg acgaaccctg catttcatca
     6481 acaaacactt tcatcttcgc acgtgctcgg atgccgaact cgtgtctcgc aagcggcctt
     6541 gtttgcagta ccacatcaaa cgctgccctg ctccttgcgt catcgacatc gaccgagagc
     6601 attacgcttc gaacgtcagc gcggtgatgt tgtttttgga aggaaggcac gatgaagtat
     6661 ccgcgcaggt cgagcagcga atgcgggagg catccaggag gatggacttc gaattggcag
     6721 ccgtgtatcg agatcagctt cgcgccgtgc agacggttcg tgaaacacaa cgcgtggtgg
     6781 cgatcaaaga tgtcgatcag gatgtgatcg gtttgcaccg tgacgcggat ctatgtgaac
     6841 ttgcgctgct gttcatacga aagggccgcg tggtggatac ggcgaccatt ccgatgcaca
     6901 aggtggaagt cggggatgag gaagtcatcg gagccttcct cttgcactac tacggtgagc
     6961 aggggcccgc ggcatttgcg atcccggatg aggtcatcgt tcccgtgctg cccgaaagtc
     7021 caaagggcgt tcaagaatgg ttgacggagc gtcgaggaag aaaagttcaa ttgctccacc
     7081 cgcaacgagg cccccgctct caattgctag cgcttgccat ggacaatgct gcgcatgcgt
     7141 ttgccgaaaa acgtcgtgca aaggatgacg tgcaggagcg tctgttggat ttgcagcgtc
     7201 gccttcgatt acccacgatt ccgcgtgtgg tggagtgttt ggatatctcc caccatgcag
     7261 gaaaagatac ctcggggggc ttggtgcgca tggtcgacgg gcagttcgat acgacgggat
     7321 accgttccta tcacgtgcat cacgcggccg aaggcgatga ttatgcagcg atttacgacg
     7381 tgttgtcgcg acggtttcgt cgggccatgc agcaggagca agagcgatcg gatgaggagt
     7441 cgcggcacga tgatccgagt aagcgtatgc cctgggagag tccggacttg ttggtggtgg
     7501 atggcgggcg gggtca
//
LOCUS       DAOSOR010000264         7509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9822_length_7509_cov_4.726858, whole genome shotgun sequence.
ACCESSION   DAOSOR010000264 DAOSOR010000000
VERSION     DAOSOR010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7509
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9822_length_7509_cov_4.726858"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PK710_14225"
     CDS             complement(<1..667)
                     /locus_tag="PK710_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10931.1"
                     /translation="METGRLRLGELLVDARIISREQLESVLQLQKKDGRRLGSLLVEQ
                     GLINETQLTQILSQQLAVPWVSLYHVDFSKKLLSLVPSHVAQKYCLIPIYVRHLRGQG
                     NTLYVAMDDPTNATALHECASWSGLPTRAMIASPSDILQAIEIYYGVASSCAETVREG
                     TSHTPAPSPAAQATTPAIADEPPEIQSDEPSHEPLAAESRTEQASEQPSIQQPETTSA
                     AQPT"
     gene            838..3483
                     /locus_tag="PK710_14230"
     CDS             838..3483
                     /locus_tag="PK710_14230"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT10932.1"
                     /translation="MRICPQCSEGCDASHRFCPACGYPVGAVSASDSDPLIGTTLPGG
                     YVILEVIGVGGMGKVYRAEQTALGRTVAVKIVHPSLLSDENASARFITEARTSSLLNH
                     PNSVSVIDFGKREAGQLYLVMEYLRGRDLARVMYEEGPLSFIRIIDILRQVLAALSEA
                     HHLGIIHRDLKPENIVIEPMRTGGDFVKVVDFGLAKILSGDKNPGITATGIVCGTPDY
                     MSPEQGRGDPLDARSDLYSVGVILFQLLTGRLPFEAESPTQVVLMHLTTPAPDPRAVA
                     PERDIPPALALVATSALAKKREDRYQDADAFSAALMAVKAQFQPAPIVSGFPNDATTS
                     CAVCGATVPRTQRFCGECGAPTGISSKPTPRMSIEPTHLALPDSGVHLPLVGRDEDLA
                     WLADRHREASEKLVAARVIGPSGVGKTRLIETFVDGLSPDHHLIVVTGPDPWWAGVAF
                     YALQKVIRDLTEPLGLGVSNGLADVPEDVHEGLAVIRQRSSPSGVDSLQVRRSVAAAL
                     RWSLRKAADHHPGHRLVVCIHDLHRIDGPSRNAFADVVGEALDRSVLVIAEHITGFDA
                     HWPGHHPARLLQGLSPETVEPLLSGKGGLVILAPGRSVLPMFVEQMVRFTEEAGTAPP
                     NHLADLVALRVERLHAGARKVLQAIAVLGHRATVSNIATLAVDTDIERGVNELIAAGL
                     IHKNDSGIVISHSLIREVASATIPAAVRRELHAHAANLALDLEMPMEARGLHALLAQD
                     SFEALLLLDQMGEAAMRWDDVDGAVLAFRRCLEVARREIFRGELDDPLRAVVIFSRKL
                     GDALSRSGDITDADGVLREALDLTDPMGEDRSKLLFSLANLEMRRSRSSDAQRYLRQA
                     IDVAHRSGLHGLVQQYELMSRDWAS"
     gene            3706..4311
                     /locus_tag="PK710_14235"
     CDS             3706..4311
                     /locus_tag="PK710_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HOT10933.1"
                     /translation="MDSDLVEAVDKLKAILDKRTISFSLGAASRDVVDRVTQRLKLPP
                     RYRSFLLEANPTELQTVTPVESVQFIPAEELLQEQVGFSLSEDLKHLSPGKDGSWRRS
                     WIIIAYGVLGDPYFLDTSQGDAEGDCPVFTAMSGTERWDPSLAASSFVQFLQILTTAM
                     EVADGFGEAELGVDDEDVFREALAPRIRTLDPAALRAGHWT"
     gene            4358..6856
                     /locus_tag="PK710_14240"
     CDS             4358..6856
                     /locus_tag="PK710_14240"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT10934.1"
                     /translation="MRVLIKVGRRCNNDCVFCHAADRRRGIEPLALLESKIRRARDKG
                     ATMVILSGGEPTVHPDLLPLAECVAHHGLQLGLVTNGRRLADASLVDDLLRHNLTFVY
                     VSLHGARASLHDAMVRTKAFEQTLRGIHQVAGRVQELTVNTVVTRDNLFELRQIVELL
                     EPLPNIGVKFTFPQPKGAAFEHFDAVIPSLSEAAHYVADAISSAQARGSPVRFGFEGF
                     PLCLMPRLESLHDDLLTRNIRSMMEPEDEDLFPVDDLLFTKLPQCENCENRAACPGVY
                     REYIRRRGDEEIVGDAHAATSHIVVEQAHLREQAAHERRHWVRLSWACNNRCRFCLDR
                     ELSRASHRSEQDIGNDILQGRREGAERLILSGGEPTTHPKFISWIRFGKRAGYGWVQA
                     ITNGRMLSYPRFLAEAIRAGLDEITVSLHGHTASLHDQLVGVSGAFEQAVRGLRSALA
                     SKRLVVNVDIVVNAHNVDHLPEMLETFVGWGVREFDLLHLIPFGGAWESTSGDLFYDL
                     AQHGDSIRRALEFCREQGVQVWLNRFPPPHAEGFEYLIQDPVKLQDEVRGRASSFEAL
                     VTGGPHLPCRDPKRCRLCYLQGLCDDFERFVALSRESFVPALRVDENVPKELLARLPK
                     TDRLEIVAADPQSALALASTLHARSVSLRLDDYEDLSKYVDDGGCFGGMALREVVVSR
                     SGQWKAALEGANTDLVVLLHRDLMPLFASFHECANRLILEQPTYHKLTTSLRNDVDLR
                     SLFQTLGKAIRTRGIARCLSGLEPLKRWDWLEARFMRPDGTIDPQQLTRWYGRDRFFA
                     KSLRCKDCRWNDDCVGMHINWLRAHGFAQLIPQH"
     gene            complement(6861..>7509)
                     /locus_tag="PK710_14245"
     CDS             complement(6861..>7509)
                     /locus_tag="PK710_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10935.1"
                     /translation="VSGFVAHWPQHGGVCHCIGVRILFVIPFLAIVPMSCKASVQTDV
                     HVGTHESAQQADEFDEAPRVADVQPTLRTEYFGIARRMTLRPAQRTATCSCVAAVVGS
                     GDDPNFEWYGDKPDIGPDALVVGVSAEGIPCEHRGRGPSIAAVDREGQDVVIVLEEFK
                     DTRPIAAGAIIPNPGPSGSIYLRAQGKAPYGRPEAGRGLRGLCKIGTGSETTNVP"
BASE COUNT         1485 a         2158 c         2166 g         1700 t
ORIGIN      
        1 ccgttggttg agctgctgag gtggtttccg gctgctggat ggacggctgc tcgcttgcct
       61 gttccgtgcg ggattctgcg gccaatggtt cgtgggacgg ttcgtccgat tggatttccg
      121 gtggctcgtc ggcgatggca ggtgtggtgg cttgcgccgc aggggaagga gcgggagtgt
      181 gcgaggtgcc ttctcgaacg gtttccgcgc aagacgaagc cacgccgtag taaatctcga
      241 tggcttgcag gatgtcggaa ggggaggcga tcatggctct tgtgggcaag ccggaccaac
      301 tggcgcactc gtgtaacgcc gtggcgttgg tgggatcgtc catggccaca tataaggtgt
      361 tgccctgtcc ccgcagatgg cgaacgtaaa tggggatcag gcaatacttc tgagccacat
      421 gcgagggcac caagctcaac agctttttcg aaaagtccac gtggtacaga gaaacccacg
      481 ggaccgccag ttgttgcgac aggatttgcg tgagttgtgt ctcattgatc aatccttgct
      541 cgaccaacag cgaccccagc cgtcgaccgt ctttcttttg cagttgcaat accgattcga
      601 gttgttctcg agagatgatg cgcgcatcca ccaacaactc accaagacgt agtcggccag
      661 tttccatcat ggatcagggt agcgcaaccc cgcgaaagtg gccgaattgt tgatgatatt
      721 gtggatgagg ggcagaaagg aatattttcc agccatatcg gaactgttat cctttctcct
      781 cgaatcgccg ccagttgttt cctaaacgcg agatcatggt aaggtgcgag atctcagatg
      841 cgaatatgcc cgcaatgttc ggaagggtgt gatgcttcac accggttttg ccctgcctgt
      901 ggctatccgg ttggtgcggt tagcgcctcg gattccgatc cgctgatcgg aacgacactt
      961 ccgggaggct acgtcatcct tgaagtcatc ggtgtgggcg gaatgggcaa ggtgtatcgc
     1021 gccgagcaaa cagccttggg gcgtacggtt gccgtcaaaa tcgtccaccc ctcccttttg
     1081 tcggacgaaa acgcttcggc acgattcatc accgaggccc ggacatcgag tttgctcaat
     1141 catcccaact ccgtatccgt cattgacttc ggaaaaaggg aagcgggtca actctatttg
     1201 gtcatggaat acttgcgggg gcgtgacctg gcccgcgtca tgtacgaaga aggaccgctg
     1261 tctttcatcc gcatcatcga tattttgagg caagtgctgg cggctctgtc cgaagcccac
     1321 caccttggaa ttattcaccg agacttgaaa cccgaaaaca tcgtcatcga gcccatgcgc
     1381 acggggggag atttcgtcaa ggtcgttgat ttcgggttgg ccaagattct ttccggagac
     1441 aaaaaccccg gcattaccgc caccgggatt gtttgcggaa cgccggatta tatgtcgccg
     1501 gaacagggcc gcggtgatcc tctcgatgca aggagtgacc tgtattccgt cggtgtgatc
     1561 ctattccagt tattgacggg aagactgccg ttcgaggcgg aatctccgac gcaggtggtg
     1621 ctgatgcatt tgacgacccc cgcgccggac ccgcgtgcgg tggctcccga gcgtgacatt
     1681 ccgccggccc tcgcgttggt ggcgacgagt gctttggcga aaaaacgcga ggatcggtat
     1741 caggatgccg acgctttttc cgcggcgctc atggccgtca aagcgcaatt ccagccggcg
     1801 ccgatcgttt ccgggttccc gaacgatgcc acgacttcat gtgccgtctg cggcgcgacg
     1861 gtgccacgaa cccagcggtt ttgtggcgaa tgcggagcgc ccaccgggat ctcctcgaaa
     1921 cccacccctc ggatgtccat cgaaccaacc cacctcgccc ttccggactc cggggttcat
     1981 ctgcccttgg tggggcgcga cgaagacctg gcatggcttg cggaccgcca tcgtgaagcg
     2041 tcggaaaagc tcgtggcagc ccgcgtgatc ggaccttccg gcgtcggaaa aacccgattg
     2101 atcgaaacgt tcgttgatgg tttgtccccc gaccaccatc tcattgtcgt gaccggccct
     2161 gacccatggt gggcgggagt agcgttttat gcgcttcaaa aagtcatccg tgacttgacc
     2221 gaaccgcttg gtcttggtgt atccaacggg ctggcggacg tgcccgaaga cgttcacgaa
     2281 ggactcgcgg tgattcgcca gcgatcttca ccgagtgggg tcgactcctt acaggttcga
     2341 cgatccgtgg cggcggcgtt gcgttggtcc ttgagaaaag ccgcggacca ccatcctgga
     2401 caccgccttg tcgtctgcat tcacgacttg catcgtatcg atggaccgag ccgcaacgcc
     2461 ttcgccgatg tggtggggga ggccctggac cgttccgtgc ttgtgatcgc tgagcacatt
     2521 acgggatttg atgctcactg gccggggcat catccggctc gtttgttaca agggctgtcg
     2581 cctgaaaccg tcgaaccttt gctttccggc aaaggagggc tggtcattct ggcgccaggc
     2641 agaagcgtgt tgccgatgtt cgtcgagcag atggtccgtt tcaccgagga ggcaggaacg
     2701 gccccgccca accatctggc cgacctcgtg gctttgcgtg tggaaagact ccacgccggc
     2761 gctcgaaaag tcctacaggc aatcgccgta ttgggtcatc gagccaccgt atcgaacatc
     2821 gccaccctgg ccgtggatac ggatatcgaa cgcggcgtga atgaattgat cgctgccggt
     2881 ctgattcaca aaaatgactc cggtatcgtc atttctcatt ccctgatccg agaagtcgca
     2941 agcgccacca ttccggctgc cgtacgacgc gagctacacg cacacgctgc caaccttgcg
     3001 ctcgacctcg agatgcccat ggaagccaga ggcttgcatg cgttgctggc gcaagactcc
     3061 tttgaagcct tgctcttgct cgaccagatg ggcgaagccg ccatgcgatg ggacgatgtg
     3121 gatggcgctg tcctcgcctt ccgccgatgc ctcgaagtgg cgcgtcgcga aatcttccgc
     3181 ggtgaactcg atgaccccct tcgtgccgtt gttattttta gccgaaaact gggagatgcg
     3241 ctctcccgct ccggggatat tacggatgcc gacggtgtgt tgagggaggc cctggatctc
     3301 accgatccca tgggggaaga tcgatctaaa ctattgtttt ctttggcaaa tcttgagatg
     3361 cgacgcagcc gatcttcgga tgcgcagcgg tacttgcgtc aggccatcga cgtggcgcat
     3421 cgctcggggc tgcatggatt ggtgcagcaa tacgagctta tgagtcggga ttgggccagt
     3481 tgactgccgg cgagagaagg ggaaacgtca atttttagcg taaattacgg tcgggggcag
     3541 ggtgagattc ctgttttggc tttggtgctt cgatgtgtcg ttgggtccgt ggcgtgaggc
     3601 atcgtcataa tttgatagtt gcccccttcg ctccggaggc aacttgcgat agactcttcc
     3661 gccctgcata gccgcgtacc ttttttcttc taagagggtt caacggtgga cagcgatctg
     3721 gtcgaggccg ttgataagct caaggccatt ttagacaaac ggacgatttc attctctctc
     3781 ggcgcagcat cgagagacgt tgtggaccgc gttacgcaac ggttgaaact tccacctcgt
     3841 tatcgctcct tcttgttgga ggcgaacccg acagaactgc aaacggtgac tccggtcgag
     3901 agtgtccagt tcattcccgc tgaggagttg cttcaagaac aggtgggctt ctccctatcc
     3961 gaggacctca aacacctgtc tcccggcaaa gatggctcgt ggcgtcgaag ttggatcatc
     4021 atcgcctacg gtgtgcttgg ggacccatac tttttagaca cctcccaagg tgatgccgaa
     4081 ggagattgtc ccgtatttac cgccatgagc ggaacggaac gatgggatcc cagcctcgcg
     4141 gcctccagtt tcgttcaatt ccttcaaatc ctgacgaccg ccatggaggt tgccgacggg
     4201 tttggggaag ccgagcttgg ggtcgacgat gaggacgtgt ttcgggaagc gttggcgcca
     4261 cgaattcgaa cgctcgatcc ggccgccctg cgtgcgggcc attggacgta attcgccgtc
     4321 gctggcttct atcctccgca tgttctaaac cgttgttgtg cgtgttctga tcaaggttgg
     4381 ccgtcgctgc aacaacgatt gcgttttctg tcacgccgcc gaccgacggc gcgggatcga
     4441 accgctggcc ttgctcgaaa gcaaaatacg ccgggcccgc gacaaaggcg cgaccatggt
     4501 catcctgtcg gggggtgaac ccaccgtgca tccggacctg ctaccacttg ccgaatgtgt
     4561 ggctcaccac gggttgcaac ttggtctcgt caccaacggt cgacgtcttg ccgatgcttc
     4621 cctggtcgac gatctgctgc ggcacaattt aacattcgtc tacgtttcgt tgcacggcgc
     4681 gcgcgcttct ctgcacgatg ccatggttcg cacaaaggct tttgaacaga cgctccgcgg
     4741 gattcatcag gttgctggac gcgtgcaaga gctgactgtc aacacggtcg tcacgcggga
     4801 caacctcttc gagcttcgac aaatcgtcga attgctcgag ccccttccca atatcggtgt
     4861 caaattcacc ttcccccagc ccaagggtgc tgccttcgag catttcgatg ccgtgattcc
     4921 ctccctgtcg gaagccgccc actatgtcgc ggacgccatc tcttccgccc aggcccgcgg
     4981 ttctcctgtg cgattcggat tcgaaggttt tcccctgtgt ctcatgccaa gactcgaaag
     5041 ccttcacgac gatctgctca cgcgcaacat tcgcagcatg atggaacccg aggacgagga
     5101 cctgttcccg gtcgatgacc tcttgttcac caaactgcct caatgcgaaa attgcgagaa
     5161 tcgtgctgca tgtcccggcg tgtaccgtga gtacattcgt cgaagagggg atgaggaaat
     5221 cgttggtgat gcccacgcgg ccacgagcca catcgttgtg gaacaggccc acctacgcga
     5281 gcaagctgcg catgaacgac gccattgggt gcggttgtca tgggcttgca acaatcgttg
     5341 tcgattctgt ctcgatcggg aactgtctcg tgcttctcat cgctccgagc aggatattgg
     5401 aaacgatatc cttcagggac gtcgtgaggg cgccgaacgt ttgatcctgt ccggaggaga
     5461 gcccacgacc cacccgaaat tcatatcatg gattcggttt ggaaagcgtg cgggctatgg
     5521 ttgggtgcaa gccattacga acggacggat gctgagttat ccgcgatttc tcgccgaggc
     5581 gattcgagcc ggcctcgatg agatcacggt atcccttcac ggacacacgg catccttgca
     5641 cgatcagctc gtgggtgttt caggcgcatt cgaacaagcc gtgcgcggcc ttcgatcggc
     5701 cctcgcgtcc aaacgactcg tggtcaatgt cgatatcgtc gtcaacgccc ataacgtcga
     5761 ccatttaccc gaaatgctag aaaccttcgt aggttgggga gttcgggaat tcgacctgct
     5821 tcatctcatt ccgttcggtg gcgcttggga atccacgtct ggcgacctgt tttacgacct
     5881 tgcccaacac ggggattcga ttcgacgcgc cctcgaattt tgccgagagc aaggcgtgca
     5941 ggtatggctc aatcgatttc ctccacccca tgccgaaggg ttcgagtatc tcattcaaga
     6001 tccggtcaaa ctccaagatg aagtgcgagg aagagcctcg tccttcgagg cgctcgtgac
     6061 cggaggaccc catcttcctt gccgagaccc caaacgctgt cggctttgtt acttacaagg
     6121 attatgcgac gattttgagc ggttcgtggc cttgtctcga gaatcattcg tcccggcgct
     6181 tcgcgtggat gaaaacgttc cgaaagaact gcttgcacga ttgccgaaaa ccgatcgact
     6241 cgaaattgta gccgccgacc cgcaatccgc ccttgcgctc gcttccacac tccacgctcg
     6301 tagcgtgtcg ttgcggttgg acgattacga ggatttatcg aaatacgtcg atgacggcgg
     6361 gtgcttcgga gggatggcgc tacgcgaagt cgttgtatcc cgatcgggtc aatggaaagc
     6421 ggcgctcgaa ggtgccaaca ccgatctcgt cgtgctcctt caccgtgacc tgatgcccct
     6481 gtttgcttcc ttccacgaat gcgcaaatcg tctcatcctc gagcaaccga cgtatcacaa
     6541 acttacgaca tcccttcgaa atgatgtcga tttgcgttcg ttgtttcaga ctctcggcaa
     6601 agcaattcgc actcgcggta tcgcccgttg tctttccggc ctggaaccgt tgaaacgatg
     6661 ggactggctc gaagcgaggt tcatgcgacc cgatggtacg attgatccgc aacagttgac
     6721 ccgttggtat ggtcgagaca gatttttcgc gaaaagccta cgatgcaagg attgtcgctg
     6781 gaatgacgat tgcgtgggaa tgcatatcaa ttggttgcgt gcccacggat tcgcacagtt
     6841 gatcccccag cactgatgca tcaggggacg ttggtcgttt cgcttcccgt cccgatcttg
     6901 cacaaacctc gaagcccacg tcctgcctcg gggcgaccgt acggcgcttt gccttgagcg
     6961 cgaagataaa tcgaacccga gggaccagga ttggggatga tggctcccgc cgcgatcggg
     7021 cgcgtgtcct tgaactcctc gagaacgatc acgacgtctt gcccttcccg atccacggcg
     7081 gcaatcgatg gcccccttcc tcgatgctca cacggaatcc cttccgcact aacccccacc
     7141 acgagcgcat cgggcccaat atcgggcttg tctccatacc actcgaagtt gggatcatcg
     7201 cccgaaccca ccaccgcggc gacgcaggaa caggtcgcgg ttcgctgtgc cgggcgaagt
     7261 gtcatacgcc tcgcgatacc gaaatactcc gttcgaagag tcggttggac atcggcgacc
     7321 cgaggcgcct cgtcaaattc gtctgcctgc tgcgccgact cgtgagttcc cacgtgaaca
     7381 tcggtttgca ccgaggcttt gcaggacatc gggacgatag ccagaaatgg gatgacaaat
     7441 agaattcgca cgccgataca atggcacact ccgccgtgct gtggccaatg agcgacgaaa
     7501 ccagataca
//
LOCUS       DAOSOR010000265         7502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9839_length_7502_cov_4.207063, whole genome shotgun sequence.
ACCESSION   DAOSOR010000265 DAOSOR010000000
VERSION     DAOSOR010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7502
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9839_length_7502_cov_4.207063"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PK710_14250"
     CDS             <1..452
                     /locus_tag="PK710_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10936.1"
                     /translation="VFEPPPMPPVLAPPVFEPPPVPPVLVPPVFEPPPMPPVLVPPAL
                     VPPAPAVGLPAAPAAPAEPPAALEESFSSVEQAAKAVPTAQITNTIALIEYLLALYPM
                     GAKDTPAGDFEGKEFLLVYDFMVAAGVRASMRSCSRHSERSNPSGSW"
     gene            466..1425
                     /locus_tag="PK710_14255"
     CDS             466..1425
                     /locus_tag="PK710_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10937.1"
                     /translation="MLYSLTVFRVGGLAIDWLVLALCVGCGEVPSGYASNAKNSDAGA
                     ETGACAPKTCVQLDVTCGLVLDGCGGMLDCGTCAQGQTCGGAGPNKCGIGVCTPKTCV
                     QLGAACGSLSDGCASVVDCGECEDGKICGGAGIPNQCACVPQVTCASMGAECGSIVDD
                     CGNSLSCGTCGSGLYCGIQTANRCDPEPCTPVSCQMYGFECGIIDDGCGGTLDCGPCA
                     STELCFSNVCCVSHAFQPCTYSCQVLMKTVLGYSQYESVWCSSEDQSLASIANCSEPD
                     PDPCTTYHLEPNCGLIPGYRWCDVTCYRHTQCDGTADCYGNCG"
     gene            1522..3015
                     /locus_tag="PK710_14260"
     CDS             1522..3015
                     /locus_tag="PK710_14260"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT10938.1"
                     /translation="MTRKKPESSSDDSDWLTTAEVAALLRVHPKHVYRLLHQGMPARR
                     VGGQWRFSRDAIQRWADQRGEPLAESSSGTAATTTTLIAAEANEVSRAVVAALAQSTG
                     LRLATVASSTTESIEALSSGEVTAAIVRAVEPVQAACATVRIALGSRIVGLLKPVAAT
                     TAEGVVAIPATLAYLPKKRTAQDVACSFLPVDGELDACASLIRGDVDAAFGSEAVARQ
                     FGLGFEPILSEPWQLVVRVDAMEEPALSRLCIAAQTGLQTHLEKVLSDVAEHGGRMHL
                     ERGIQPGPSQAVAISDTWVAEKPSRKAPMAVRWTILARDRAEQMLDLVDVLRHRGVSV
                     GGFLQLPSGPASAKPMGYDLFRLRHPERLPIAERNLTGRRNQGERFCELTFHKNALVR
                     ACEWLREDVETSDVLIVDGVGRLEQRGQGLFPALAWARMQPRSTMIVLSSRWNRVPQV
                     TQRLALSGPQVTELSFGHHDATPPHIIEHIMRACKKSPRSHRVVEGS"
     gene            3012..4100
                     /locus_tag="PK710_14265"
     CDS             3012..4100
                     /locus_tag="PK710_14265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT10939.1"
                     /translation="MTVQRHLPSTRWSTAMGTGKSSILRRAVAEAIEAGERPITWLPN
                     VSDVGLRTERVQFPDMDVATRLGAEVFSGKLIRNLSSGERQRVRLALALSTQGGLVAF
                     DEPCRHLDPEHVEALSRLLLDRAGSGTRVLVSDLRRQLSPEIFEEDIGENGDDGPVRP
                     VSSPEGRDSLGVDIPIPFSRAYQGKPRGTMRLELEKGSLIGLRGRNGSGKTSLLEALA
                     RALRQAGKSVGYSRQEPEHQVFACTPKAELNALSHRYLSKNIDDFANRLDIESWLDTP
                     TVRLPLGVLSLLGTWIALEMGRDCVLLDEPSQGLDLRRARILAEELAAFAGQGARIVV
                     ASHDPELIRVATRTWSVREGILEVDGER"
     gene            4097..4693
                     /locus_tag="PK710_14270"
     CDS             4097..4693
                     /locus_tag="PK710_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10940.1"
                     /translation="MRNLETYRFWGYAATFGALWGAVEITVGSFLHALRIPFSGVVLA
                     GFGAALLIALRRVFPVPGVMIAAGLVCAGVKLLSPAGAVVGPMVAILAESLLVEATVR
                     WRGATILSGSVAGASAALWSVLQKLVTQTLFFGMPVLGIYQGIARQAERTLRLPASEG
                     LAAVGLFLSIVVIGGAMFGVVGAWVGKATCRRLVQAES"
     gene            4690..5298
                     /locus_tag="PK710_14275"
     CDS             4690..5298
                     /locus_tag="PK710_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10941.1"
                     /translation="MNFGRLSLLVAVALLVGLLLVPAVWHLPIGIAGWWILRWGARDV
                     HRNLGRPWRWLQVLFVLTVLGGFFGPADTQWLSVSWSWAGVLAGCTMVVRAFALVSLM
                     SLITSVLPVRRWVERVQHPVLRRLIEVVVVAANLVPVQLNALGVASMTLKERRPGLLN
                     FPLRIWLLAVHSALHAAMLAESVALDMAIAAHNASDQGKISS"
     gene            5295..5783
                     /locus_tag="PK710_14280"
     CDS             5295..5783
                     /locus_tag="PK710_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10942.1"
                     /translation="MKFPLHLVLFAVTMGLTAACGDEQSVDSGQPSPSNDGATGVIVT
                     VTLENKSVPVDLGTLGTTAYKGVELVKLSDVWAASGIGVDPTTLEFEFVSADGFRPSS
                     KGCDDLAGSNIDKGFIHPTSRNITWDETLGLRGCYAVNDTEQMNGHVPEKKTGDTDAA
                     DR"
     gene            5780..>7502
                     /locus_tag="PK710_14285"
     CDS             5780..>7502
                     /locus_tag="PK710_14285"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT10943.1"
                     /translation="MKRAAAVGVLLAFLGTANAWSHPSAPDSDEALPEDEEDPSGEEP
                     RPLEVVVQGRRPGTRGTSVQKLGASTLRQWGTTSVSEALERLPSAVAASGGRGERIIS
                     LRGFDQRQILITIDGAPIQVPYDGQLDLGKFPLGLVDHVTVVKGSGSLLLGPNGLGGA
                     VDVATRRAGEGPMLSLAAETAPFSAERLSAVASGNQGPVGLLAGAAYENVLYYPLSDS
                     FAPTFNEGGGRRENSDRRSLSFLGKARWQVDEEQEVAASLWRLDGRFGVPPGVYDLSR
                     RFWRWTDWHVDTYTVAHGYRGEHLTTDETLYISMVGNTLDIYDGADYHWQELPASGTS
                     VYDDRVMGGNARIAYRFSCRDRCVTARGWVGGKRDIHRTRDKDDAPWIEVGTTTISVA
                     GQVDGPLGEPASWLVGTQVDAEFPDAIASGVAPKSAMGVGPMAALNWQPGRMLDMTAS
                     VAHRTRFPTLKERFSSAFGLLAPNPALSPERATNTSVDATFRPIEGLRVDVGIFESEV
                     TDLVVQVPREQGMLQWQNTGRARIYGIESVLLTRPTPGLEMWGGWAAMNTRRLDGPAE
                     RIAYRPEH"
BASE COUNT         1388 a         1960 c         2489 g         1665 t
ORIGIN      
        1 cggtgttcga gcccccgccc atgccgccgg tgctcgcgcc cccggtgttc gagcctccgc
       61 ccgtgccgcc ggtgctcgtg ccgccggtgt tcgagcctcc gcccatgccg ccggtgctcg
      121 tgcctcccgc gttggttccg cccgcccctg ctgtcggact tcccgcggcg ccggctgccc
      181 ctgctgaacc tccagcagcg ctggaagaat cgttttcgtc ggtcgaacag gcggccaagg
      241 cagtgcccac ggcacaaatc acaaatacaa tcgccctcat cgagtacctc ctcgcgcttt
      301 atccgatggg agcgaaggat acaccggctg gtgatttcga agggaaggaa tttttgctgg
      361 tctacgattt catggttgcc gcaggggtca gggcttcgat gaggagctgc tcgagacatt
      421 ccgagcgaag caacccttcc ggttcatggt gaacaggtct cggccatgct atactcgctc
      481 accgtgtttc gagtcggtgg ccttgcaatc gattggcttg tccttgcgct ctgcgtgggc
      541 tgcggcgagg tgccatcggg atatgcgtcc aatgcaaaaa attctgatgc aggtgcggaa
      601 acaggggcct gcgcgcccaa aacctgcgtg caactcgatg taacctgcgg actggttctc
      661 gatggttgcg gagggatgct ggattgcggt acctgcgcac aaggtcagac ctgtggtggc
      721 gcaggtccta ataaatgcgg gattggagtc tgcaccccca aaacctgcgt gcagcttgga
      781 gctgcttgtg gaagcctttc cgatggttgc gccagcgttg tcgattgtgg tgaatgcgag
      841 gacggcaaaa tttgtggagg cgcgggaatc ccgaaccaat gcgcttgcgt gccccaggtg
      901 acgtgcgcct cgatgggggc cgaatgtggc tcgatcgtgg acgactgcgg caactccttg
      961 tcttgtggaa cctgcggatc gggattgtat tgcggtatcc agaccgccaa tcgctgcgat
     1021 ccagagcctt gcacgcctgt gagctgccag atgtatgggt tcgaatgtgg catcatcgac
     1081 gatggttgtg gcgggacact cgactgcgga ccttgtgcat cgacagagct atgtttttcc
     1141 aacgtttgtt gcgtttccca tgcctttcag ccttgcacct attcgtgtca ggttctgatg
     1201 aaaacggttc ttggttattc ccaatacgag tccgtgtggt gcagcagtga agatcaatcg
     1261 cttgctagca tcgccaattg ctccgaaccg gatccggacc cctgcacgac ctaccatctg
     1321 gagccaaact gtggcctcat tcctggctat cgctggtgcg atgtgacctg ctaccgccat
     1381 acgcaatgcg atggaaccgc ggattgctac gggaattgcg gatgatgagg ggattgaccg
     1441 tgttcttggg gaagccaccc tagacgatgt agaaaattgt tgcaagttgt tgcatgctga
     1501 ggtaggcact cttccggtcc gatgacacga aaaaaaccgg agtcttcctc tgatgacagc
     1561 gattggctga cgaccgcgga ggtggccgcc ctgctgcgcg ttcatcccaa acacgtgtac
     1621 cggctgcttc atcaaggcat gcccgcgcgt cgcgtgggag gacaatggcg tttttcccgc
     1681 gatgccatcc aacggtgggc ggatcaacgg ggagaaccgt tggcagaaag ctcatcaggg
     1741 acagcggcca ctacaacgac attgatcgcg gccgaagcca acgaggtgtc gcgggcggtg
     1801 gtagcggccc ttgcgcaatc cacggggcta cggcttgcca cggtcgcttc cagcacgacg
     1861 gaaagcatcg aagcgctttc gtctggcgaa gtgacggctg ccatcgttcg tgccgttgag
     1921 ccagtgcaag cagcctgtgc cacggttcgc attgcgctgg gctcccgtat cgtggggctg
     1981 ttgaagcctg tagcagcaac gaccgcagaa ggggtggtgg ccattccggc cacgctagcc
     2041 tatctaccca aaaagcgaac tgcacaagat gttgcgtgtt cattcttgcc cgtggatggg
     2101 gaactggatg cttgcgcatc gctcattcga ggcgacgtgg atgcagcgtt cggttcggag
     2161 gccgtggctc ggcaatttgg gcttggtttc gaacctatcc tatccgagcc ctggcaactc
     2221 gtcgtccgtg tcgacgccat ggaggagcca gccctatcgc gcttgtgcat tgcagcccaa
     2281 accggattgc aaacgcatct ggaaaaggtg ctgtcggatg tggccgagca tggggggaga
     2341 atgcatctgg aaagagggat tcagcccggg ccgtcgcaag cggtggcgat aagcgacaca
     2401 tgggttgcgg aaaaaccatc acgaaaagcg ccgatggccg tgcgctggac cattttggcg
     2461 cgggaccgag cagagcaaat gcttgatctt gttgatgttt tacggcatcg gggagtaagt
     2521 gtgggcgggt ttttgcaact tccctccgga cccgcttcgg ccaaaccgat gggctatgac
     2581 cttttccgcc ttcgccatcc ggaacgtctc cccattgccg agcgaaactt gaccggccgt
     2641 cggaaccaag gagagcggtt ttgcgaactg acctttcata aaaacgcgct ggttcgagct
     2701 tgtgaatggt tgcgggaaga tgtggaaacc tcggatgtgc tcatcgtcga tggcgtaggg
     2761 cggctcgagc agcggggcca ggggcttttc ccagcccttg cctgggcgcg aatgcagccc
     2821 cggtccacca tgatcgtgct ttcgtcgcga tggaaccggg ttccccaggt cacccagcgc
     2881 ctggctcttt ccggacctca ggtgacggaa ttatcctttg ggcaccacga cgcgaccccc
     2941 ccgcatatca tcgaacatat tatgcgcgct tgtaaaaagt cacctcgttc ccaccgtgtt
     3001 gtcgaggggt catgactgtc cagcgtcacc ttccctcgac tcggtggtcg actgccatgg
     3061 gcacgggaaa gtcgtcgatt ctccgacgcg cggtggccga ggccatagaa gccggggagc
     3121 gtcccatcac ctggctgcca aacgtatcgg atgtaggtct tcggacggaa cgagtgcaat
     3181 ttcccgatat ggacgtggcg actcgtctgg gagccgaggt tttttcggga aaacttatcc
     3241 gaaacctgtc atccggggag cggcaacggg ttcggttggc cctggcgctc tcgacccaag
     3301 gggggctcgt tgccttcgac gagccgtgtc gtcacctgga cccggagcat gtcgaggcgt
     3361 tgagtcgtct acttctcgat cgtgcggggt cggggacgag ggtgttggtg tcggatcttc
     3421 gtcggcagct ttcgccggag atatttgagg aggatatcgg ggaaaacggg gatgatgggc
     3481 cggtgcggcc ggtttcgtcg cccgagggca gggattcgct aggagtggat attcccatcc
     3541 cgttttctag ggcgtatcag ggaaaaccgc gaggaaccat gcgtctggag ctggaaaaag
     3601 gctcgttgat cgggcttcgc ggacgaaatg gcagtggcaa aaccagtttg ctcgaagcgc
     3661 tggcgcgcgc tctccgtcag gcaggaaaga gcgtgggata tagtcgtcaa gagccggagc
     3721 atcaggtatt tgcctgcacg ccgaaagcgg agttgaacgc gctgtcgcat cgctatctat
     3781 ctaaaaatat tgacgatttc gcgaatcggt tggatatcga atcatggctg gacacgccca
     3841 cggtgcggct ccctcttggg gtactcagtc ttctcgggac atggattgcg ctcgagatgg
     3901 ggcgtgattg tgtgctactc gatgagccga gtcaggggct cgacctgcgc cgggcgcgaa
     3961 ttttggcgga agaattggcg gcttttgccg ggcaaggggc gcggatcgtg gtggccagcc
     4021 atgatccaga attgatccgg gtggcgactc ggacctggag cgtacgtgaa ggaatccttg
     4081 aagtcgacgg tgagcgatga gaaacctgga aacctatcga ttttggggat atgcagcaac
     4141 gtttggtgcg ctgtggggag ccgtggagat cacggtcggc tcgttccttc acgcgctaag
     4201 aattcctttt agcggtgtag tcttggcggg gttcggcgcg gcgctgctca ttgctttgcg
     4261 acgagtcttt ccggttcccg gggtgatgat agctgcaggg ttggtgtgcg cgggcgtgaa
     4321 attgctttct cccgccgggg ctgtcgtggg gcccatggtg gcgatactgg cggaatcgct
     4381 gttggtggag gccacggttc ggtggcgagg agcgacgatc ctttccggta gcgtggcggg
     4441 agcttcggcg gctttgtggt cggtgctcca aaaactcgtg acccagacgt tattcttcgg
     4501 gatgccggta ttggggattt accaagggat cgcgcggcag gccgaacgga ccctgcgtct
     4561 gcctgcctcg gagggactcg cggctgtcgg gctatttctt tccatcgtgg tcattggtgg
     4621 cgcaatgttt ggtgtcgtcg gagcatgggt gggaaaggcc acgtgccgtc gactcgttca
     4681 ggcggaatca tgaacttcgg acgtctctcg ctgctcgttg ccgtcgcgtt gctcgtggga
     4741 ttgctgctcg tcccggcggt gtggcatctg cccatcggca tcgcgggatg gtggatcttg
     4801 cgttgggggg cccgagacgt ccaccgcaat ctgggtcgac catggcgatg gctgcaagtg
     4861 ttattcgtat tgactgtctt gggaggtttt ttcggccccg cggacacgca atggctttcc
     4921 gtttcgtggt cttgggcagg tgtccttgcg ggttgcacca tggtcgttcg agcttttgcg
     4981 ctggtttcac ttatgtctct catcacttcc gtgctccccg ttcgtcgctg ggtcgaacgg
     5041 gttcagcatc cagtcctgcg aagactcatc gaggttgtcg tggtcgcggc gaatctcgtt
     5101 ccggtgcaac tcaacgctct gggggttgcc tcgatgacct tgaaagagcg acgccccggt
     5161 ttactcaatt ttcccctgag aatctggcta cttgccgtgc atagcgcgtt gcacgccgcc
     5221 atgctcgcgg aaagcgtggc cttggatatg gccattgccg cccataacgc gagtgatcaa
     5281 ggaaagatat cgtcatgaag ttccctttgc atttggttct gttcgccgta acgatggggc
     5341 ttactgccgc ctgtggagat gagcaatcgg ttgactcggg tcagccgtcg ccatcgaacg
     5401 acggcgcaac cggagtgatc gtcacggtga cgttggagaa taagtcggtt ccggtggatc
     5461 tgggaaccct ggggacaacg gcttataaag gggtcgaatt ggtcaagctc tcagatgtct
     5521 gggcagcctc cggaattggg gtggatccaa cgactctcga gttcgaattt gtgtcggccg
     5581 atgggttccg gccatcgagc aaaggatgcg atgaccttgc gggaagcaac atcgacaaag
     5641 ggttcatcca tccaacgagc cgcaacataa cgtgggatga aacactcgga ttacgaggtt
     5701 gttatgcggt gaacgatacg gagcagatga acgggcacgt accggagaaa aagactggcg
     5761 acacggacgc tgccgatcga tgaaacgcgc tgcggccgtc ggggtattgc ttgcttttct
     5821 ggggacggca aacgcctgga gccatccttc ggctccggat agtgacgagg ccttgcccga
     5881 ggacgaagag gatccatccg gggaagaacc tcgtcctctg gaagtcgtgg tgcaggggcg
     5941 ccgtccggga actcgtggaa cgagcgtgca aaaactcggt gcctcgaccc ttcggcaatg
     6001 gggaaccacg agtgtttcgg aggcgttgga acgattgccg tccgcggtag ccgcatcggg
     6061 tggccgtggg gaaaggatca tatcgttgcg gggcttcgat cagcgtcaga tattgataac
     6121 gattgatggc gcaccgatcc aggtcccgta cgatgggcag ctcgacttgg ggaaattccc
     6181 gcttggattg gtggatcacg tgacggtcgt gaaagggtct gggtcgttgt tgctgggtcc
     6241 caatggtttg gggggagcgg tagacgtggc aacgcgacgc gccggcgagg ggcctatgct
     6301 ttctctggcg gcggagacgg ctccattttc ggcggaacgg ctttcggccg tcgcctcggg
     6361 aaatcaaggg ccggtgggcc tgcttgcggg ggcggcttac gaaaacgtat tgtattatcc
     6421 gctttccgat tcgtttgcgc caacgttcaa tgaaggaggt gggcgacgag aaaactcgga
     6481 tcgtcgaagc ctgtctttcc ttgggaaggc gcgatggcag gtggatgagg aacaggaggt
     6541 ggcggcgagt ctttggcgtc tcgatggccg atttggggtg cctcccgggg tttacgacct
     6601 gagtcgtcga ttttggaggt ggactgattg gcatgtggat acgtatacgg tggcccatgg
     6661 ttatcgagga gagcatctga cgacggacga gacgctatat atcagtatgg tgggcaatac
     6721 cctggatatc tacgatggag ccgattacca ttggcaggag cttccggcat cgggaacgag
     6781 cgtctacgac gatcgcgtga tgggtggaaa tgcccgaatt gcttatagat tttcttgtcg
     6841 ggaccgttgt gtgacggccc gaggttgggt gggggggaag cgagatatcc atcgtactcg
     6901 ggacaaggat gacgcgccgt ggatagaggt gggaacgacg acgatatcgg tagcggggca
     6961 ggtggacggt cctttggggg agccggcgtc gtggcttgtg ggcacgcagg tggacgcgga
     7021 gtttccggat gccattgcat caggtgttgc gccaaaatcg gccatggggg taggaccgat
     7081 ggcggcgttg aattggcagc caggcaggat gctcgacatg acggcaagtg tcgcgcatcg
     7141 aactcgtttc ccgactctca aagagaggtt cagctcggct tttgggttgc ttgcgccgaa
     7201 tcccgcgttg tctccggaaa gggccacgaa cacaagcgta gacgcaacgt ttcgtccgat
     7261 cgaagggctt cgggtcgatg tggggatatt cgaatccgag gtgacggacc ttgtggtgca
     7321 ggtgcctcgg gaacaaggca tgttgcaatg gcagaatact ggacgggctc gcatctatgg
     7381 tatcgaatcc gttctgctca cgcgccccac gccagggctc gaaatgtggg gggggtgggc
     7441 cgcgatgaat acgcgaaggc ttgacggtcc ggccgaacgg attgcctatc gtccggagca
     7501 ta
//
LOCUS       DAOSOR010000266         7429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_9986_length_7429_cov_4.342826, whole genome shotgun sequence.
ACCESSION   DAOSOR010000266 DAOSOR010000000
VERSION     DAOSOR010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7429
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9986_length_7429_cov_4.342826"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..801)
                     /locus_tag="PK710_14290"
     CDS             complement(157..801)
                     /locus_tag="PK710_14290"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT10944.1"
                     /translation="MGSSNTSSRIARRRAAQRAAILSIAAQRFAEHGVDRVRLDQIAD
                     EADVARNTLYSHFSTKEALVEAIVRPGLEQGLSALRKLRGKEPRQRIDGVLEAYLQLW
                     RQHRDSLRVASRLQSFPSGNLAELHQAFAQGVLTALRSAARAGILRVKNPTIAARLVM
                     RVAIPLLELYLGYPNAEQLFHDSMQGLLLLDQPPPKTPTKPSRNPKKQTRPSSR"
     gene            914..2161
                     /locus_tag="PK710_14295"
     CDS             914..2161
                     /locus_tag="PK710_14295"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT10945.1"
                     /translation="MAEHRSSKLVTLPRIALLLVALTVIMVFFWHRSRGPLVQVVAAQ
                     RHAVVDRVVASGKVLPEARIHLGTVVLGTVVEVSAREGEGVKKGQVLIGLDDTEAQAL
                     VQQAKASLQQAEARLGQVGGVSARVALEAVAQAAAKKEQARSDLERERSLAEQGASTQ
                     ERVDAASKAFEIASSAHAAALAQSSSLGPGGGDHRAAVSQVALARAMLDAAKARLAQT
                     RVVAPAEGIVLTRDVEPGDVVQAGRTLMTLAQAGWTGLTVQVDEKNLSLLRLGQRATA
                     SADAFPLESFEAIVDYIAPSIDAGRGTIEVRLRVDKPPSYLRTDMTVSIAIDAGTRQN
                     VLLVPGAAVRDATTPSPYVFLLEENHLSKRDIRLGTRAGDAVEVVAGLEGGEPVVLTL
                     DVGLQQGQRVRSHVVSEMQYRAF"
     gene            2151..3368
                     /locus_tag="PK710_14300"
     CDS             2151..3368
                     /locus_tag="PK710_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT10946.1"
                     /translation="MLFEWFVSLRFLRAGKTQTLLIFVGVAFGVAVMVFVGALIDGLQ
                     ANLIRQTLSAQPHVIVRPPERDPRDASAKTDALQALRVEKAVQHTATIDQWQVVEQIV
                     RSVPGVTATAPTVAGSAFVSKGSISRSVALRGVDPTTYIQIIPIDQKIRRGSFRLVGT
                     EAVIGTELAKDLGVSVGDKLRMVSTEGRDEIVSISGVFDIGNKDLNQRWVFVSIRSAQ
                     TLLELPGSVSTIEAKVNDVFRADWVASQAAARTGLDADSWMKLNAQLLIALKSQSSSS
                     YMIQFFVMLTVMLGIASVLVVSVVQKSKEIGILKATGTTTRSITKIFMIQGAMVGIVG
                     SLLGSLVGTGLSLLFASLAKNSDGSPTFPVDLGPTRFALAFGVALTVGLLAAVAPARR
                     AASLDPAKVIRYG"
     gene            3361..4062
                     /locus_tag="PK710_14305"
     CDS             3361..4062
                     /locus_tag="PK710_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT10947.1"
                     /translation="MADTLLRLQNVVKAYGNRVLTTVIHGVDFTVTQGELTALIGPSG
                     SGKSTLLNLMGLLDRPTSGLVEVRGQDTSRLDDLALARFRGRSIGFVFQFHHLLPAFT
                     ALENVLLPLWSDRGRVSRVERQHAYSLLERVGMADYAKHKATDLSGGQQQRVAIARAL
                     VMDPAIVLADEPTGNLDSQTADKVFSLLREFGREKGCAMIVVTHDARIAARCDRVVKL
                     VDGRIVSDERRESTT"
     gene            complement(4109..5134)
                     /gene="tdh"
                     /locus_tag="PK710_14310"
     CDS             complement(4109..5134)
                     /gene="tdh"
                     /locus_tag="PK710_14310"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HOT10948.1"
                     /translation="MKALIKAKPEVGIWMGEVPKPEIGPNDLLIRIRKTAICGTDIHI
                     YHWDDWAQKTIPVPMVVGHEFAGEVVEIGSAVEGFHIGDRVSGEGHITCGYCRNCRAG
                     KRHLCRNTKGVGVNRQGCFAQYLSLPAFNAFKLPDSISDDQAAVFDPFGNAAHTALSF
                     DLVGEDVLITGAGPIGCMAAAICKHVGARFVVVTDVNPYRLELASKLGASRVVNVRQQ
                     KLEDVMNELGMTEGFDVGLEMSGTPSAFQQMLATMNHGGRIGLLGIPPKETLIDWNHV
                     IFKGLIIKGIYGREMFETWYKMSAMLQSGLNIQPIITHRFAIDDFQAGFDVMRSGQSG
                     KVVLCWD"
     gene            complement(5310..7355)
                     /locus_tag="PK710_14315"
     CDS             complement(5310..7355)
                     /locus_tag="PK710_14315"
                     /inference="COORDINATES: protein motif:HMM:NF021379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HOT10949.1"
                     /translation="MDNHSETTEALTPMAEPSTANEGPGESNTSEPSEGEKAPRNSSN
                     YAETIGKILRSLTAFGLMGFSSVLYLQQLRGKWVDKFIIGNTLDIEARNLLLLTLVLG
                     AGFAMVLPGAFLLWKRSQAAVERIQRLARILSPLVVVGAIPAIFRWKPWTEEPIGLSV
                     AILVVGFILEKTLRLSLEAFPENRWRGLAHRLIAIRDRFPRLTHYAPLLIVVAGSLFY
                     AIFMSVMALRLHWRLETRAFDLGGYQSLFVNALSGHPFRCPANVMPTGDWSSLKGHAE
                     ISIYALLPLYALYPRAETLLVIQSSLLGLAAIPLFLTLARHLPRSLAAVVALGYLAFP
                     SLQAANLFDFHMQPVAIFFVLWVIYFLDARRYVWLAIALPIALGCREDIGIGLTIGAI
                     FLVFSGYRPIAGAIVALVSVCYFVVMKFILMPMAGEWWFENIYKDLFPPGQATYFGVV
                     KTLLTNPIFVLKTLLTQDKLIHILRILTPVFFLPLRRTALWWLLIPGAFFTILTTGYK
                     PTVSPTFQYVGHWIPYLFVATGLALAAIGKYQGKIHQAAASITLIVVTLACTYNWGAM
                     MQRNHMVAGWGRVQLGPLTEEEKVKLQQLEELIALIPVDASVGASETEHPHFYQQITV
                     QTLRYSYDKPDYILYRINTGKFGSDHGKKQVNEGNYVQIESRGPFILLERKDRISKN"
BASE COUNT         1512 a         1850 c         2442 g         1625 t
ORIGIN      
        1 tgggcggggt gagccgacgc gagcgacaag gcgaccaggg gaatcgatag ggagcgaggg
       61 ttgagcacga ggggagtata gaggcatttg ggaaaaggcg gatcggcgtt acggggcagc
      121 agtgtttctg aataatttca atatgttgag cgcaaattac cgagacgagg ggcgcgtttg
      181 ttttttggga ttgcggctgg gtttggtggg ggttttaggg gggggttggt cgagcaggag
      241 caagccttgc atgctgtcgt ggaacaattg ttcggcgttg gggtaaccga gatagagttc
      301 gagcaagggg atggcgacgc gcatgacgag gcgagcggcg atggtgggat ttttaacgcg
      361 caggattccg gcgcgggcgg cggagcgcaa ggcggtgaga acgccttggg cgaaggcttg
      421 atgcaattcg gccaggttgc cggaggggaa gctttgcaga cgggaggcta cgcgtaagga
      481 atcgcggtgt tggcgccaaa gttgaagata agcctcgaga acaccgtcga ttcgctggcg
      541 aggttccttg ccccgcagtt tgcgaagggc ggacagtccc tgttcgaggc cagggcgaac
      601 gatggcttcg acgagcgctt ctttggtgga aaagtggctg tacaaggtgt tgcgcgccac
      661 atccgcttcg tcggcgatct ggtctaggcg aacgcggtcc acgccatgct cggcgaagcg
      721 ttgggcggca atggagagaa tggcggcacg ctgcgcggcg cgacgtcggg cgatccgaga
      781 ggatgtgttg gagctaccca cgaaggcagc ttgacaatat gatctcattg tgtcaactat
      841 gaactcatga gttcaagttc gaatcctttc gttcgaatct ttgactcccc tgccaccgca
      901 ggttgtttcc ctcatggctg aacatcgctc ctccaagctc gtgaccctgc cgcgaatcgc
      961 gctgctgctc gtcgccctga ccgtgatcat ggtgtttttt tggcatcgct cccggggtcc
     1021 gttggtgcaa gtcgtcgccg cccaacgaca tgctgtcgtc gatcgggtgg tggcctcggg
     1081 caaggttctt cccgaggcgc ggattcatct gggcacggtc gtgcttggga cggtggtgga
     1141 agtgtccgcg cgcgaaggcg agggcgtgaa aaaaggtcag gtattgatag gcctggatga
     1201 tacggaggcc caggctctcg tgcagcaggc caaagcctct ttgcagcaag ccgaggccag
     1261 gctgggacaa gtcggcggcg tgagcgcgcg tgtggcgctc gaggccgtgg cgcaagcggc
     1321 cgccaaaaaa gaacaagcgc gttccgatct cgagcgagaa cgatcgttgg ccgagcaagg
     1381 ggcttccacg caagaacggg tcgatgcggc aagcaaagcg ttcgagatcg cctcgagcgc
     1441 gcatgcggca gccctggcgc aatcatccag tctcgggccg ggaggtgggg atcatcgcgc
     1501 ggcggtctct caagtcgccc ttgcccgtgc gatgctcgat gccgccaagg cgaggcttgc
     1561 gcaaacccgt gtcgtggctc ccgccgaggg aattgtactc acgcgtgatg tcgagcctgg
     1621 agacgtcgtg caggccgggc gcacgctcat gacgcttgcg caggcgggct ggacggggtt
     1681 gacggttcag gtcgatgaaa agaacttgtc gttgcttcgt cttgggcagc gggcgactgc
     1741 ttcggcggat gcattcccct tggaatcctt tgaagccatt gtcgattata ttgccccttc
     1801 catcgacgcg ggtcgtggga cgatcgaagt tcgacttcgt gtcgacaagc cgccttcgta
     1861 tttgcgaacg gatatgactg tatcgattgc gatcgatgca ggtacgcgac aaaacgtatt
     1921 gctcgttccg ggagcggcgg tgcgcgatgc cacgaccccc agtccgtatg tttttctgtt
     1981 ggaggaaaat cacctgtcca aaagggatat tcgtcttgga acgagagcgg gtgacgccgt
     2041 ggaggtggta gcgggcctcg agggtggcga gcctgtcgtg ctgaccttgg atgtgggttt
     2101 gcaacaaggc cagcgcgttc ggtcccacgt tgtatcggag atgcagtacc gtgctttttg
     2161 agtggtttgt ttccttgcgg ttcttgcgag ccggcaagac gcaaacgttg ctcatcttcg
     2221 tgggagtggc gttcggtgtg gccgtcatgg ttttcgtggg agctttgatc gacggcttgc
     2281 aagccaattt gattcgtcaa acgctcagcg cccagcccca tgtgatcgtt cgtccgccgg
     2341 agcgtgaccc tcgtgatgcc tctgccaaga ccgatgcgct tcaagcgctt cgggtggaaa
     2401 aggcggtgca gcatacggcg accatcgatc aatggcaggt agtcgaacag attgtccgaa
     2461 gcgtgcctgg ggtgaccgcc accgcgccga ccgtggcggg ttcggctttc gtcagcaaag
     2521 ggagcatcag ccgttcggta gccttgcggg gcgtggaccc caccacatac atccaaatca
     2581 tccccatcga tcaaaagata cgtcgcggtt catttcgtct ggtgggaacc gaagctgtca
     2641 tcggcactga gcttgccaag gatctggggg tgagtgtggg agacaagctg cgcatggtga
     2701 gcacggaagg ccgcgatgaa atcgtatcga tttcgggtgt tttcgatatc ggcaacaagg
     2761 acctcaacca gcggtgggta tttgtctcca ttcgttcggc gcaaaccttg ttggagttgc
     2821 ccggcagcgt gtcgaccatc gaagccaagg tcaacgatgt gttccgcgcg gattgggttg
     2881 cctcgcaagc cgccgcccgc acgggactcg atgccgatag ctggatgaaa ctcaacgcgc
     2941 agcttctcat cgccctcaag tcccaaagca gttccagcta catgatccaa ttcttcgtca
     3001 tgcttaccgt catgctcggt atcgccagcg tgctcgtggt atccgtcgtg caaaagtcga
     3061 aagaaattgg gatattgaaa gcgacgggga ccacgacgcg cagcatcacc aagatcttca
     3121 tgattcaagg agcgatggtg gggattgtgg ggtctttgct cggatcgctg gtgggcacgg
     3181 gcctatcgtt gttgtttgcc agtcttgcga aaaactcgga tgggtcgcct acctttccgg
     3241 tggatttggg gcccactcga tttgctctcg cctttggtgt ggctctcacg gtgggtcttt
     3301 tggcggcggt ggcaccggct cgtcgcgccg caagtctcga cccagccaag gtcattcgtt
     3361 atggctgata cgttgctccg gctgcagaat gtggtaaaag cgtatggaaa tcgtgtactt
     3421 acaacggtca tccacggtgt cgatttcacg gtcacgcagg gagaactcac cgctctgatt
     3481 ggaccctcgg ggtctggaaa aagcaccttg ctcaacttga tggggctgtt ggatcggccc
     3541 acgtcgggcc ttgtcgaagt gcggggtcag gatacctcac ggttggacga tcttgcgctg
     3601 gcgcgatttc gcggacgctc gattggattc gtctttcaat tccatcatct attgcctgca
     3661 tttacagcgt tggaaaacgt tttgctgcct ctttggtcgg atcgggggag ggtttcgcgg
     3721 gtggaacggc agcatgccta ctcgctgctc gaacgggtgg ggatggcgga ttacgccaag
     3781 cataaggcga cggatttgtc ggggggtcag cagcagcggg tggcgattgc gcgtgctctt
     3841 gtgatggatc ccgcgattgt gcttgcggac gagcccacgg gtaatttgga tagtcagacg
     3901 gcggacaagg tattttcgtt attgcgggag tttggaaggg agaagggatg cgcgatgatc
     3961 gtggtcacgc acgatgcgag gattgcggcg cgttgtgatc gcgtggtgaa gttggtggat
     4021 ggccgtatcg tgtcggacga gcgtcgtgag tctacgacat gaggggattc aggtattttg
     4081 ctggggcatt tcgatacgga gcgcgggatc aatcccagca gagcacgacc ttgccggatt
     4141 gtccggagcg catcacatcg aatcctgctt ggaagtcatc gatggcaaag cgatgggtga
     4201 tgatcggttg gatattcaat ccgctttgca gcatggcgct catcttgtac caggtctcga
     4261 acatctcacg cccgtaaatc ccctttataa tcaagccctt gaagatgacg tggttccaat
     4321 cgatgagcgt ctccttggga ggaatgccga gaagtccgat gcgcccgccg tggttcatgg
     4381 tggcgagcat ttgttggaaa gcggaaggcg tgccggacat ttcgaggcca acgtcgaaac
     4441 cttcggtcat gccgagttcg ttcatcacgt cctcgagttt ttgctggcga acgttcacga
     4501 cgcgggaagc gccgagtttt gaagccaatt cgagacgata ggggttgacg tcggtgacga
     4561 ccacgaagcg tgctccgacg tgcttgcaaa tggcggcggc catgcatccg atgggtccgg
     4621 cgcccgtgat gagcacgtct tcccccacga gatcgaagga tagggcggta tgggcggcat
     4681 tgccgaacgg atcgaaaaca gcggcttgat cgtcgctgat gctgtcgggg agcttgaaag
     4741 cgttgaaggc gggtaaggac aagtattgtg cgaagcatcc ttgacgattg acgccgacgc
     4801 ctttggtatt gcggcaaagg tgtcgttttc cggcgcggca attgcggcaa tatccgcagg
     4861 tgatatggcc ttcgcccgag acgcgatccc ctatgtggaa tccttcgacc gcggagccga
     4921 tttcgacgac ttctccggcg aattcatggc ccacgaccat gggaacgggg atggtttttt
     4981 gggcccaatc gtcccagtgg tagatatgga tatcggtccc gcaaatcgct gtttttcgga
     5041 tccgaatgag caagtcgttg ggtccgattt ccggcttggg cacctctccc atccagatgc
     5101 ctacctcggg cttagccttg atgagcgcct tcatcgcacc gacctccagt cgaagaattc
     5161 catcaaccga tgacagcgaa agccaaccgg ctggacttcg catccacggt tcgatctggc
     5221 ttgctagcac ggtggatggc cgatttcatc tgttctcgat gggcgctggg agtctgttgt
     5281 ttcggctcgt gttttcaaac gcggtgggat cagtttttcg agattcggtc tttgcgttcg
     5341 agcagtatga aaggcccgcg gctttcgatt tgaacgtagt tgccttcatt gacctgcttt
     5401 ttgccgtggt cggagccaaa ttttccggta ttgattcgat agaggatgta atcgggcttg
     5461 tcataggaat agcgaagcgt ttgcaccgtg atttgttgat agaaatgggg gtgttcggtt
     5521 tccgaagctc cgaccgatgc atcgacggga atcaacgcga tcaattcttc gagttgttgc
     5581 agcttgactt tttcctcttc ggtaaggggt ccgagttgaa cacgtcccca accggcgacc
     5641 atatggtttc gctgcatcat cgcgccccaa ttgtaggtgc aggcgagggt aacgacgata
     5701 agggtgatgg atgccgcggc ctgatggatt ttgccttggt atttgccgat ggcggccaaa
     5761 gccaggcctg tggctacgaa aaggtaaggg atccaatgcc ccacgtattg gaacgtgggt
     5821 gatacggtgg gtttgtatcc cgtggtcaag atggtgaaaa acgcaccagg aatgagcagc
     5881 caccaaagcg cagttcgtcg aaggggcagg aaaaaaacag gggtcaagat gcgaaggata
     5941 tggatgagtt tgtcttgggt gagcagggtt ttcagcacga agatggggtt ggtaagcagg
     6001 gtttttacga caccgaagta ggtggcttga ccgggaggga acaggtcttt gtagatattt
     6061 tcgaaccacc attcgccagc cataggcatg aggatgaact tcatgacgac gaagtagcag
     6121 acggatacaa gcgccacgat ggcgcccgcg atggggcggt aaccggaaaa cacaaggaaa
     6181 atcgcaccta tcgtcaagcc gatgccgatg tcttcccggc agcccagggc gatgggcagg
     6241 gcgatggcga gccacacgta gcgtcgggca tcgaggaagt agatgaccca gaggacgaaa
     6301 aaaatcgcca ccggttgcat gtgaaaatcg aagaggttgg cagcttgcaa cgaggggaag
     6361 gcgaggtaac caagggcgac gaccgcggcg agggagcgtg gaagatgccg ggccagggtg
     6421 aggaataggg ggatggcggc cagaccgagc agcgagcttt ggatgacaag gagcgtttcg
     6481 gcgcgggggt acagggcata cagggggagc aaggcgtaga tcgagatttc cgcatgtcct
     6541 ttgagcgaag accaatcgcc cgtgggcatg acattggcgg ggcagcggaa cggatgtcct
     6601 gacaaggcgt tgacgaacag gctttggtag ccaccgaggt cgaaggctcg cgtctcgagg
     6661 cgccagtgca ggcgcaacgc catcacgctc atgaaaatgg cgtaaaacag agagcctgcc
     6721 accacgatga gcagcggggc atagtgcgta agccgtggaa agcgatcacg aatcgcgatg
     6781 aggcgatggg ccaaaccacg ccatcggttt tccggaaaag cctccaagga aagccgtagt
     6841 gttttttcga ggatgaagcc aaccacaagg atggcgacgc tcaaaccgat gggttcttcg
     6901 gtccagggtt tccaacgaaa aatggcgggg atggcaccga ccaccacgag cgggctgagt
     6961 attcgcgcga gacgttggat gcgttcgacc gcggcttgcg accgtttcca caacaaaaat
     7021 gcgccaggca ggaccatggc gaatcccgcg cccaacacca gggtcagcag caacaggttg
     7081 cgcgcttcga tatcgagggt gttgccgatg atgaacttgt cgacccactt gccccggagc
     7141 tgttgcaagt acagcacgga ggaaaaaccc atcagtccga aagccgtcag gctgcgaagg
     7201 attttcccta ttgtttctgc atagttagat gagttccggg gggctttttc cccctcggaa
     7261 ggttccgacg tattcgattc ccctggccct tcgttcgccg tggatggctc tgccatggga
     7321 gtcagcgctt cggtagtctc agaatggtta tccatcgtgg tgcgacaaag cgaatcagcg
     7381 tgttaggcca ttttgaccgg tggttagcct agcaggtcgg ttgcgatgg
//
LOCUS       DAOSOR010000267         7405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10034_length_7405_cov_3.742585, whole genome shotgun sequence.
ACCESSION   DAOSOR010000267 DAOSOR010000000
VERSION     DAOSOR010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7405
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10034_length_7405_cov_3.742585"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PK710_14320"
     CDS             <1..560
                     /locus_tag="PK710_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10950.1"
                     /translation="RCIVFGQILAREKASLIATTFMAYEAPQISFEGPCCALFALGSS
                     HHPPEVKPHVDGAGRSWNCDIRFYPGYRSKNTPVSNRPWDPGADPEEYARSGLYPPDW
                     IEVNLDRQREDGSKLVQRRYALTVAGRALNFETLSERMTNLVWMLRTALEIPHYSPDA
                     VQRVREAWKERCNSQEAQLLHLATV"
     gene            588..2177
                     /locus_tag="PK710_14325"
     CDS             588..2177
                     /locus_tag="PK710_14325"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOT10951.1"
                     /translation="MMGVGDFFKKPIRDLFIASPQGAAGSIIWRYPEQSIPSGAKVTV
                     RSDETAVFFKDGKSMGILEAGVYSLDSRNIPFLTEWLVSPMTGGNYCVTELFFVRRSE
                     ILHGTGSRELGSFSDVGTQLLVTLFFQARFGLQVHQPVTLIETLAGQRVTAGGQVEEW
                     LNGRVGSLMSAVVGQIMATAPVMQVVSNQYSEQIGQFVIDNARHQFDHQGLRITRFVD
                     LRLQLSGESRQELNKISHQRATLAVEREEMELARDPGYVRYHLVKGQRAMLEGIGKGA
                     ASGAGMAMMALPPLMAPLPQPVVTPSFRAENTPLQNLGVHRPRQLTTSQRYFLRLGHN
                     MQGPYNKRQLILHAEALGVGPSEIEIRQEGSTNWQILDEIDELAEAWMSRPSQQDKPR
                     SSGQPASQGFESALKVALQDGELSNEALALLVSLACASGLAVDAAAARAYVISRARAC
                     GAKVPESEAEKPKAAAPPPIPVGPPVFSYSNGIQQLDGLTAEAIARRVQALPDGVHLV
                     WRTGMDGWKSASEVEEIASLL"
     gene            2884..3837
                     /locus_tag="PK710_14330"
     CDS             2884..3837
                     /locus_tag="PK710_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10952.1"
                     /translation="MGLLLSTHGGWVLAEEPVPEKANKHQDAAIAESLFLQGRQAAEE
                     LRWEEACAKFEASLRLVRTLGSLANLAWCHENRGKSASAWAVYRDLLSAAHDKGDTKR
                     ENFALQRLQDLEPRLARIQIIVNHSPEGLQVRFGDQVLAEGAWNCAIPVDPGSLHLRA
                     EAPGYISQDWTVEVPDGVRIIETEVPMLRRVVTPPVKARETEATKEVSTETGDDTLAY
                     IFAATAVAGLGVGAYSGIMTLVKKAEGDELCTGRYCSEEGLEAHESSKRYANVATISI
                     GAALLLGGGSVWLFASGSQDPARSASVSTNNGYYFQVGTVF"
     gene            complement(4067..5617)
                     /locus_tag="PK710_14335"
     CDS             complement(4067..5617)
                     /locus_tag="PK710_14335"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT10953.1"
                     /translation="MMPSFSPQDLIGCLVDGRYRLETLAGQGGFGSVYRAHHVRFCGS
                     VAVKILRIPSYLDAEARKCLVASFEGEGRLLFELSALHPAIVQVKESGVLFFQGLSFP
                     YLVMEWLDGLTLETDIRNRQSEGYGPRDLEEAIQILNPIAEALALVHSQGIAHRDIKP
                     GNVFLSRVGTALTPKLLDFGIAKVLSPAGTQSAMYAPTSDGRGAFTELYGAPEQWFCR
                     LGATGPWTDVYAFALLLVEMLSGKRPHLVQTTKQLAGAAIDPEIRPTPRAAGVDVPDA
                     VETVFQRALAVDPRERPRSVGEFWHELLEASRFVPSAFATPLPYDTTVLLPAKPQDPI
                     SVHPAENGIMTGVTESASHPPTSRTPPLGLHPPVSGDFNPKIIGPHPPSRRQIVGKWL
                     SPIAVSVVLALLTLLGLSRSSTPRPQAASQPTFSEKPSPNPLTTQIMAAPIPSASISR
                     STMIQPAAMPAPTFDQPQNARPPVRRSPIMPAKPAHSNASPSIAAPTNDSSEPATLSV
                     HDVLQSWR"
     gene            complement(5614..6972)
                     /locus_tag="PK710_14340"
     CDS             complement(5614..6972)
                     /locus_tag="PK710_14340"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT10954.1"
                     /translation="MSDLTQTNQAVTQGATPPRGNRLSSKKQGEPGLVQIFPREADAA
                     PATWKITHRDVAGRSSECPWRLEDTKVSRKHLVLEPAPEGFLVSDLGSRNGTYIDGVP
                     LGSGSQALVHFGSVLRVANTLLLAVPTVQSYATPPRAFSAASGVSKAVVAGPTLSAVW
                     DEAARIAGEPHPVLVVGENGSGKEVLAQIIHAVGHPDKPLVARNVGAIPEGLFESELF
                     GHARGSFTTAVQSRLGAFRSANHGVLFLDEIADLRLDHQVKLLRALDDGLIMPVGEDR
                     PIKVNTRVVAATNKDLRQLVIEGRFREDLFFRLATVTLRVPPLRERKDEIILLALRML
                     GAQSGGFRMSTDTAEVLALAPWPGNVRNLLRVMLEACRQATKENRSIIEPSDLPDLEP
                     LGSDDSENRPVLTTEKLRTAVLRSHGVIKRAALSLGISRSSFYEECKRLGIDPRELRK
                     RS"
BASE COUNT         1606 a         1733 c         2462 g         1604 t
ORIGIN      
        1 gacgatgtat cgtgttcggt cagatactgg caagggaaaa ggcatctttg atagccacga
       61 cattcatggc ctatgaggcg ccgcagattt ccttcgaagg gccgtgttgt gcgttatttg
      121 cgctgggatc gagtcatcat cccccggagg tgaagccgca tgtggacggt gcggggcgct
      181 catggaattg cgacattcgt ttttatccag gttatcgttc caaaaataca ccggtatcga
      241 atcgtccttg ggatccgggg gccgatccgg aagaatacgc gcgctcgggg ctttatcccc
      301 ccgattggat cgaggtgaat ttggatcggc aacgggagga tgggagcaag ttggtgcagc
      361 gtcgttatgc cttgacggtg gcggggcggg cgctaaactt tgagactctg tcggagcgta
      421 tgactaattt ggtttggatg cttcggacag cgttggagat acctcattac agtccggatg
      481 cggtacaacg ggtacgagag gcgtggaaag aaaggtgtaa cagtcaggaa gctcagttgt
      541 tgcacctggc aaccgtataa tttccagaga gactggaaag gaagacgatg atgggtgttg
      601 gggatttttt caagaaaccg attcgcgacc tattcatcgc ttctccgcag ggggctgcgg
      661 ggagtattat atggcggtat ccggagcaat cgattccttc gggggcgaaa gtgaccgtac
      721 ggtcggatga aacggctgtt tttttcaaag acggcaagtc gatggggatt ctcgaggctg
      781 gagtgtattc gctggacagc agaaacattc catttcttac ggaatggctc gtttccccta
      841 tgacgggggg caactattgt gtgacggagt tattttttgt tcgtcggagt gaaattttgc
      901 acggtacggg gtcgcgggaa ttggggagtt tttcggatgt tggcacgcag ctcctggtga
      961 cgttgttctt tcaggcgcgt tttgggcttc aagtgcatca gccggtgacg ttgatcgaga
     1021 cgttggcggg gcagcgagtg acggcggggg ggcaggtgga ggaatggctc aacggacggg
     1081 tgggttcgct gatgagcgcg gtggtggggc aaatcatggc aacggcgccc gtgatgcagg
     1141 tggtatccaa tcagtattcg gagcagattg ggcagttcgt gatcgataat gcgcgtcatc
     1201 aatttgatca ccaggggctt cgcatcacgc gtttcgtgga tctgcgattg cagctttcgg
     1261 gcgagtcgcg tcaggaactc aacaagatat cgcaccagag ggcgacgctg gcggtggagc
     1321 gggaggagat ggagcttgcg cgggatccgg gctatgtgcg atatcatttg gtgaaaggcc
     1381 agcgggcgat gctggagggg attgggaagg gtgcggcctc gggcgcgggg atggcgatga
     1441 tggcgttgcc gccgcttatg gctcccctgc cccagcctgt ggtcactcca tcgtttcgag
     1501 cggaaaatac gccgcttcag aacttggggg tgcatcgtcc tcgccagttg acgacctctc
     1561 agcgctattt tttgcgtttg gggcataaca tgcaggggcc ttacaacaag cggcaattga
     1621 tactgcatgc ggaggcgctt ggagttggac cgtcagaaat cgaaatacga caagagggga
     1681 gcacgaactg gcagatactt gacgagatcg atgagctagc ggaagcgtgg atgagccggc
     1741 cgagccagca agataagcct agaagttctg gtcaacctgc gagccaggga tttgagtcgg
     1801 cgctgaaggt agcgcttcaa gatggggaat tgagcaacga ggcgctcgcg ctgttggtgt
     1861 cgctggcgtg tgcgtcgggg cttgcggtcg acgctgctgc tgctcgggct tatgtcatca
     1921 gtagggcgcg tgcgtgtgga gcgaaagttc cggagtcaga agccgagaag cccaaggcgg
     1981 cggcacctcc tccgataccg gtggggccgc ctgtattcag ttattcgaat gggatccagc
     2041 agttggatgg gctaacggca gaagcgatag cgcgtcgtgt gcaagcctta cccgatggag
     2101 tgcatttggt gtggcgaact ggcatggacg ggtggaagtc cgcgtcggaa gtcgaggaga
     2161 tcgcgtcgtt gctttgaacc cgcgggcaga atgctggtgg gattttccaa agacgattgg
     2221 aataccggtg cgggagcata gaccatatag tcatgcagca acgttttata cataccacaa
     2281 cgtattgtgt cgattctgga gttgattgcg ggtattgggc cttggcgttt cggggaattg
     2341 tcgagggaag gatggtggtt ttggaggttg cgcttccgtc atggatgagg cggcgtcggc
     2401 gctttgtgct accagcaacg ttttatgatt ttggatagga atgagatgat gggggtagag
     2461 gcgtggaagg agctacgagc ggaatagccc atggcaaggg tccaagtcag ccagttttcg
     2521 ttcacgaagc catccggaga agggcaacgt caatgtcgaa gacttcgggg gaaatgagtt
     2581 ttcgatacca ggattgcgaa aaaaaatgcg taggggaaaa ggaaaggatg cctcaggtgt
     2641 agtagaaaaa tgcggggctg gtcgtgggga aaaaggacga gattccttcc gtatttctct
     2701 gtaaaggcca agttcccctt accttgggat aacgttcatc atattctggc tcagataccc
     2761 aattcttgat aaaaaatgat gacgatgcga ggaaattcgg gacttatccc ttcggcggac
     2821 atggcaagct gggggcaccg gacggcacgc ttttgtgcca ggcttctcga atattcgttc
     2881 tggatgggat tgctcctctc cacgcatggc ggttgggtat tggccgagga gcccgtcccc
     2941 gaaaaggcca acaaacatca ggatgcggcc attgcggagt ccttgtttct tcaaggccgg
     3001 caggctgcgg aagagctgcg atgggaagaa gcctgtgcga aattcgaagc gagtcttcgt
     3061 ttggttcgca ccttgggttc tcttgccaat ctcgcttggt gccatgaaaa ccgcggaaag
     3121 agcgcatcgg cgtgggcggt ataccgggat ttgctgtccg cggcccacga caagggggat
     3181 acgaaacgag agaatttcgc gttgcaaagg ctgcaagacc tcgagccacg gctggcgagg
     3241 attcagatca tcgtgaacca ctctccggaa ggattgcaag tgcgctttgg cgatcaggtc
     3301 ctggcagaag gtgcttggaa ttgtgctatt cccgtcgatc cgggaagcct tcacttacga
     3361 gcggaggcac ccggctatat atcccaggat tggactgtcg aggtccccga tggggtgaga
     3421 atcatcgaaa cagaagttcc gatgcttcga cgggttgtga ccccccccgt gaaagcacgg
     3481 gagacagaag caacaaagga ggtgtcgacg gagacagggg atgacacgct tgcctatatc
     3541 tttgcggcca ccgcggtcgc gggattggga gtgggtgcgt attcgggaat catgacattg
     3601 gtaaaaaaag cggagggcga cgaattgtgc actggcaggt attgcagtga agaggggctc
     3661 gaggcccatg aatcctccaa gcgctacgcg aacgtagcca ccatttcgat aggtgcggct
     3721 ttgctcttgg gagggggatc cgtctggtta tttgcctctg gctcgcagga cccggctcgc
     3781 tctgcctcgg tttcgaccaa taatggctat tattttcaag tgggtaccgt cttttgacct
     3841 cttgtcccgg ttggcgctgt tttcatgaac atgacgttcg agggaatcgt ggcagaaggg
     3901 ctccggctgt atgtatctcc ttattttaaa aggcatttct tgtgattggc atggcggaat
     3961 tggctttcga ctgttcgcca gggctcggag cttaggtatt tcgacctgag tggggttcgg
     4021 gccgcctcgg cgcaacagag gggagatgaa acgaggattg gatgctttat cgccacgact
     4081 gcaggacatc atgaacggag agggtggcag gttcgctact gtcgttcgtt ggcgcggcga
     4141 tcgagggtga ggcgttggaa tgagcgggtt tggcgggcat gataggggat cgtcgaactg
     4201 gcgggcgagc gttttggggt tggtcgaagg tcggcgcggg catggcagca ggctgaatca
     4261 tcgtggagcg ggagatcgaa gccgagggaa ttggggcagc catgatttgc gtagtaagag
     4321 ggttaggcga aggcttttcg gagaaggtgg gttggctggc ggcttggggc cgcggggttg
     4381 aagatcggga caatccgaga agggtaagca gcgcgagcac gacggatacg gcgatgggag
     4441 acaaccattt tcccacgatt tgtcggcgag agggggggtg gggcccgatg attttggggt
     4501 tgaaatcacc ggaaaccggg ggatggagtc cgaggggggg agtacgcgag gtgggaggat
     4561 gggaggcgga ttcggtaaca ccagtcatga taccgttttc cgcggggtgt acggaaatgg
     4621 ggtcttgcgg ttttgcggga agcagaacgg ttgtatcgta gggaagtggc gtggcgaacg
     4681 cagaggggac aaatcgggaa gcctctagga gttcgtgcca gaattccccg acggatcgcg
     4741 gtctttcgcg ggggtccacc gcgagtgctc tttggaaaac ggtttcgacg gcatccggga
     4801 cgtcgacgcc tgcggcgcgg ggggtggggc gaatttccgg gtcgatggcg gcgcctgcga
     4861 gttgtttggt ggtctgcacg agatgcggcc gcttgccgga aagcatttcg accagaagaa
     4921 gggcgaaagc ataaacatcg gtccagggcc ccgtggcacc cagtcggcag aaccactgtt
     4981 cgggggcgcc atatagttcg gtaaatgcgc cgcgtccgtc ggaggtggga gcgtacatgg
     5041 cgctttgggt gccagcggga gacaggactt tggcgattcc gaagtcgagt agtttggggg
     5101 tcagagcagt ccctacacgg gataaaaaga cgttacccgg cttgatatca cggtgggcaa
     5161 ttccttggga gtggacgagg gcaagggctt ctgcgatggg attgaggatt tggatggcct
     5221 cttcgaggtc gcgagggcca tatccctcgc tctgtcgatt gcggatgtcg gtttcgagcg
     5281 tgaggccgtc gagccattcc atcaccagat agggaaagga aaggccttgg aagaagagaa
     5341 caccgctttc tttgacttga acgatggccg gatgaagtgc ggaaagctcg aaaagcagtc
     5401 gcccctcacc ttcgaaagaa gccacgaggc attttctcgc ctccgcatcg agataagagg
     5461 gaattcgcag gattttcaca gctacggagc cgcagaaccg cacatggtga gcgcgataga
     5521 cggagccgaa accgccttgt ccggccaggg tttcgaggcg ataccggccg tccacgaggc
     5581 atccgatgag atcctggggg gagaacgacg gcatcatgat cgttttcgaa gctcccgggg
     5641 atcgatacca agccgtttgc attcctcgta aaaggaggat cgcgagatac ccaggctaag
     5701 agccgcgcgt ttgatgacgc cgtgggagcg caggacggcg gttcgcaact tctccgtcgt
     5761 caagacggga cgattttcgg aatcgtccga gcccaatggc tcgaggtcag gtaaatccga
     5821 gggctcgatg atggagcgat tctccttcgt cgcctgccga caggcttcga gcatcacacg
     5881 cagcagatta cggacgttac caggccaagg ggccagggcc agcacttccg ccgtatccgt
     5941 ggacattcga aaaccgccgc tttgagcccc aagcatgcgc aacgcgagaa ggatgatttc
     6001 gtctttgcgt tcgcgcaggg gaggcacacg caaggtcaca gtggccaggc ggaaaaacag
     6061 gtcttcccgg aaccggcctt cgatcaccaa ctgccgaagg tccttgttgg tggctgccac
     6121 cacccgggta ttgactttga taggtcggtc ttctcccacc ggcatgatga gtccgtcgtc
     6181 caaggcacgg aggagtttga cctggtgatc gaggcgtaag tcggcgattt catcaaggaa
     6241 aaggacacca tggttggcag agcgaaaggc gcccaaacga gattggacgg ccgtggtgaa
     6301 cgagcctcgc gcatgcccaa atagttcgga ttcgaaaagg ccctcgggga ttgcgccgac
     6361 attacgagcc accaggggtt tatccgggtg gccgacggcg tggatgatct gcgcgagcac
     6421 ttcttttcct gagccattct caccaacgac gagcacggga tgcggttccc cggcgatccg
     6481 agcggcttca tcccagacag cggagagggt aggtcctgct acgacagctt tcgaaacgcc
     6541 ggacgcggcg gaaaaagctc gagggggcgt tgcgtaggat tggacggtgg gaacagccaa
     6601 gagcaaggta ttggcaacgc gcaataccga gccgaaatgc acgagagcct ggctgcccga
     6661 cccgagagga acgccgtcga tgtacgtacc attacgactg cccaagtcgg agaccagaaa
     6721 tccctccgga gccggctcca agaccagatg cttacgagat actttcgtat cttcgagacg
     6781 ccaggggcat tcggagcttc gtccagcgac atcgcggtga gtgattttcc acgttgccgg
     6841 agctgcgtca gcctcgcgcg ggaaaatctg aacaagtcct ggttctccct gttttttgga
     6901 ggacaagcga ttcccccgcg gaggggtagc tccttgcgtg accgcttgat tcgtctgcgt
     6961 caggtcggac atccccgttc gctcctcaca ctggcaaggt agcgattctt ttatcaggat
     7021 ttacacgaaa tctcaagcca gggtatctgg aaaaacgcgt tatgagggga tgtgtgacat
     7081 tgaacgccca cgacgttgca tatatttaat agcttatact caatatatac tgaataagaa
     7141 cgctacaagc aggaaaatac ttattgaaaa ggcttacagg caggtagata gcgtgagatg
     7201 aagagagtcg agacagcggc gaaacttatc agtgtttgaa cataatggcg cgtttcgcgc
     7261 tggtccgagt atcgtaaaac cgtcaatgaa acaacaactt acaattatac ggaccccttt
     7321 ttcacctctt catcgtccgc cacgggaacc tcttgcggct gcgcttccgg ctcttgcacc
     7381 gctaccctca tcccgggctc cgacg
//
LOCUS       DAOSOR010000268         7398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10051_length_7398_cov_3.548686, whole genome shotgun sequence.
ACCESSION   DAOSOR010000268 DAOSOR010000000
VERSION     DAOSOR010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10051_length_7398_cov_3.548686"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PK710_14345"
     CDS             complement(<1..689)
                     /locus_tag="PK710_14345"
                     /inference="COORDINATES: protein motif:HMM:NF018165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOT10955.1"
                     /translation="MKDYQTLCYKHAENKPMPSIDALEKIVSIARSILFPGYFGNSAI
                     NSSTIKKHISRNVWELEELLTNQIFAGLCTTCDNYSEKNKTLAQTIAYQFIERLPDIR
                     KALFTDVQATYENDPAAKSSMEIIFCYPAIRAISNYRIAHELLKLQVPIIPRIITEMA
                     HSETGIDIHPGATIGNYFTIDHGTGVVIGETTIIGDHVKLYQGVTLGAKNFPIDENGK
                     PIKGIPRHPII"
     gene            complement(753..1274)
                     /locus_tag="PK710_14350"
     CDS             complement(753..1274)
                     /locus_tag="PK710_14350"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT10956.1"
                     /translation="MLKTIVITGPESTGKTTLAKELAAHFGADWVPEYARTYIETLGR
                     PYTYDDVEQIAIKQKEQFDAALNAKGNYVFFDTYLFITHVWFLHVYQREPVWIKSALK
                     ECQADLFLLCRPNIPWEYDPIRENPNNRDFFYDWYKRELLHYGKKIIEIDRLGKQRTE
                     QAIAAVNLLTDTY"
     gene            complement(1484..1930)
                     /locus_tag="PK710_14355"
     CDS             complement(1484..1930)
                     /locus_tag="PK710_14355"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT10957.1"
                     /translation="MGDDVCGLVGRTVDGKVRVDDCIGEGGFAVVYRGFHLVFEQPVA
                     LKCLKVPSHFDSEAQASFLTRFREEGKMLFRLSQHAAVVRVFDIGEVETSRGEQVPYL
                     VLEWLDGEELSDLLSRRNAQGLAQNSFIVGYPTANSNGTRHSNRDR"
     gene            2030..3415
                     /locus_tag="PK710_14360"
     CDS             2030..3415
                     /locus_tag="PK710_14360"
                     /inference="COORDINATES: protein motif:HMM:NF020678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HOT10958.1"
                     /translation="MPLRPRLEACVWSCHTARLLLESMTMAYRPFATIALLSLVFGCS
                     SESAPSSVGKEAGSDAALDAVEEPNASDDGHGDTSVTNDSNTEADVSDEPIVDGGQEA
                     KDDADAGEEPTWPGPPWFGCTPHDKPATATLVTAFDHENQYFNPQDKRTIDAVVNFPQ
                     GAWERIDLIVELTCPEDGDCDNWDRFANLMLVDNAGTANEEVLELERYITPFNIGLCI
                     RTDVTRLAPRLTGTKTLRSFIDTWVGPNEPIHGHGWRTTVQFVFHPGTPSSTTVPSSV
                     VSLWPFETIDVGDPQRPVAEQAGTRTVLIPAGTKRAELRVVATGHGQGNRFNCAEFCR
                     LEHEFTVNGTAFSFDPWRDDCSKNPIGPKQAGTWKFSRAGWCPGAYTMPTIIDITGAV
                     QPGQEATITYDSMSPIHGEYENTCRPDAGDANNKCVGCAFDNSAGNCDFNGGSHTPPH
                     HRLTTQLLLYP"
     gene            complement(3498..4784)
                     /locus_tag="PK710_14365"
     CDS             complement(3498..4784)
                     /locus_tag="PK710_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10959.1"
                     /translation="MGCSQESEDTSSSKKQGDASSGVEDSQGSQGDGSVSDGNPTDSG
                     MDTEGDILVDTSIDLPDDTAFDQSIDGVTDVLDAEETGESSSCKSHPPEVLPYDPKHF
                     SLNQPLGSAVTLDPRSDAVVERLALNTTLRAVGLGTHGESPSVYEVSDSDPLYTVHVS
                     SVPTPELFRVPAGAVQGGGSDSPSELLDRNHPAYGPFVELRLWQAVFDHTKQTITASG
                     GGLFHYNSDGKLLNPDGSPSVGTPFKGWGTGSGLSYLAGLIRPEEVIAGEICHALRFS
                     YSNCHFTDTFRPPATKTDQPKGCPLSLAERGDPASWMEMGMRLQLDPAVDCETRTVPK
                     RNASHDVTEETRMLRMICRALQRYGMVALDGTVPGGLLIYAEDTSTAAWDTILGPPDV
                     HARYSFMLRDQHSATDGFQRDEHSGIPWHRLRVVVP"
     gene            complement(5111..6775)
                     /locus_tag="PK710_14370"
     CDS             complement(5111..6775)
                     /locus_tag="PK710_14370"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOT10960.1"
                     /translation="MRNEARIDLTGQSLCIPGGVWLASGRGESGSAGGMLYSTAMTNT
                     AVLKVCGDDVRITGLRIVGPDPTTCPPQYPNQCEKTGPNCAYCTPPSLGIRNDKYVRC
                     EIDNNELTAWSYAAINLGSGADHRVHHNDIHHTQRRGLGYGVVLNATAGDAVDVLVEW
                     NRFDYNRHAIAGSGASGQDYTSRHNLVLPHSIGHVFDMHGENERVGNGDPGAGGKMLI
                     HENIVLVADQYSLAVRGRPAQGAWFYENCLARATPGEAARQLYFFGNFHVDESPSGAA
                     PNRYGQSASSCAHLRWCFAPGGSAPFAYLSRASDPMEELALGDFDGDGKTDVFRSKNG
                     RWEWSRGGVELWATLATSTITLSSLRFGDFDGDGKTDVFHATGSEWHVSYGGSSAWQT
                     LRTASESTSQLAFGDFDGDGKTDVFTASGGQWKVCFGGKGTWQSLASSGVALGSLGFG
                     DFDGDGKTDVFHGDGSAWRVSWAGTSSWEKLTDSSYGIDSLLLADIDGDGITDILRSD
                     GSTWWVSRSATQTWERLRISSLDVRKLRVGDFDGDGLPDVFQAQCL"
     gene            complement(6753..7220)
                     /locus_tag="PK710_14375"
     CDS             complement(6753..7220)
                     /locus_tag="PK710_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10961.1"
                     /translation="MHRLLLPLSFMVCFVSCGGGEEVAHAPGPGFDGGQGADDAAQGD
                     SATGTDSAPWESGQSDGPQQGLEASPDALDAPFDAPEEAAPVDCQTYRTRHDLSSPPE
                     SASWRWGGRLRLSGPARAQSVVYRRGLHAARAVECLGKRRSGYDRLCEKRGTD"
BASE COUNT         1673 a         2141 c         1881 g         1703 t
ORIGIN      
        1 tcgataatag gatggcgcgg aatacctttt atcggtttgc cattttcgtc tatcgggaaa
       61 ttttttgcgc ccaacgttac gccttggtac agtttaacat ggtcgccgat aatggtcgtt
      121 tcgccgataa caacaccggt gccgtggtca atggtaaaat agttaccaat agtagccccc
      181 ggatgtatat ctatgcccgt ttccgaatgc gccatttcgg taataatgcg cggaataata
      241 ggcacttgca attttaatag ttcgtgcgct atgcggtagt tgctgatggc gcgtatagca
      301 ggatagcaaa aaataatttc catactgctt tttgcggcgg ggtcgttttc gtaggtagct
      361 tgaacgtcgg tgaacagggc tttacgaata tcgggcagtc gctcaataaa ttgataagca
      421 atggtttgtg caagagtttt atttttttcc gaatagttgt cgcaagtagt gcatagcccc
      481 gcaaaaattt gattagtcaa aagttcttcc aattcccaaa cattgcgtga aatgtgtttt
      541 ttgatagtag aggaattgat ggctgaatta ccaaaataac ccggaaaaag gatgctgcgt
      601 gctatagaaa cgattttttc taaagcgtca atactcggca tcggcttgtt ttcggcatgc
      661 ttgtagcaca atgtttggta atctttcatg ttagccaatt gttcaatgat aaggtctaaa
      721 ttttgcttat tgttatccat aatcaaaaac ggttaatatg tgtcagtgag taaatttact
      781 gcggcaatgg cttgctctgt gcgttgtttt cccaatcggt ctatttcaat aatttttttt
      841 ccgtaatgaa gaagttcccg tttataccaa tcgtaaaaga aatctctgtt gttgggattt
      901 tctcttattg ggtcgtattc ccatggaata ttgggacggc atagcaaaaa taaatctgct
      961 tggcactctt tcagagcgga ttttatccat acaggttcgc gttggtaaac gtgtaaaaac
     1021 caaacgtgcg tgataaacag gtaggtgtca aaaaacacgt aattgccttt tgcattgagc
     1081 gccgcatcaa attgttcttt ttgttttata gcaatttgtt ccacatcgtc gtaggtgtac
     1141 ggtctgccaa gtgtttcaat gtatgttcgt gcatattcgg gtacccaatc tgcgccgaaa
     1201 tgtgctgcca attctttggc taatgtggtt ttacctgtcg attcgggacc tgttataaca
     1261 attgttttta acacaaaatt tatctacctt tgtcgcaaat aataatatta tgtgcaaaga
     1321 tattttaaaa atttcattta tcggcatatt tctgctgttt tctctgcaaa tttttgcgga
     1381 aaagccgaat ttgaatggtt tcgattttaa aggcacatat tattttaata gccattccaa
     1441 caaagcatcg cgcggttatt ctcgattttg cgatgaaaca acactaccta tctctattgc
     1501 tatgccttgt gccattgcta ttggcggttg gatagccaac gatgaaacta ttttgcgcga
     1561 gtccttgggc attgcgacgg ctcaacaggt cactgagttc ttcgccatcg agccactcca
     1621 gaacgaggta cggaacctgc tcgccgcgtg aagtctccac ttcgccaata tcgaatacgc
     1681 gtacgaccgc ggcatgctga gacaagcgaa acagcatttt tccttcctca cgaaaacgcg
     1741 tgagaaaaga cgcctgcgcc tcgctgtcga aatgggatgg aaccttgagg cacttgaggg
     1801 ctaccggctg ctcgaacaca agatgaaagc ctcgatacac cacggcaaat ccgccctcac
     1861 cgatacagtc gtccacccgc accttgccat ctacggttcg accgaccaat ccgcagacat
     1921 cgtcgcccat ggtatgcctt gcgggtaccg caagctgcca aaggggtcaa cgttcgactc
     1981 gccgcccaag cccttgccgc cgcatcttcg tggggggcac catgacacaa tgcctttgcg
     2041 cccacggctc gaggcttgcg tatggtcttg tcatacggca cggttgttgt tggaatccat
     2101 gaccatggca taccgcccct ttgcgaccat cgccttgctt tcgctcgttt ttggctgttc
     2161 gtcggaatct gccccttcct ccgtgggtaa ggaggcaggc tcagacgccg cgctcgatgc
     2221 cgtcgaggag cccaacgcca gcgatgatgg ccacggcgat acatccgtca cgaatgattc
     2281 gaataccgaa gccgatgtgt cggacgaacc catcgtggat ggggggcaag aagcgaaaga
     2341 cgacgcggat gcgggcgagg agcctacctg gccgggcccc ccctggtttg gatgcacgcc
     2401 ccacgacaag cccgcgacgg ccacgctcgt caccgctttc gatcatgaaa accaatactt
     2461 caaccctcag gacaagcgga ccatcgatgc cgtggtgaat ttcccacaag gcgcctggga
     2521 gcggatcgat ttgatcgtgg aattgacctg tccagaggat ggcgattgtg acaattggga
     2581 ccggtttgcg aatctcatgc tcgtggacaa tgccggtacc gcgaacgaag aggttctcga
     2641 attggagcgg tacatcacgc cattcaacat cggcttatgc atccgcacgg atgtgacgcg
     2701 gctcgcacca cgccttacgg gcaccaaaac cctccgctcg ttcatcgata cctgggtagg
     2761 gcccaacgaa cccatccatg ggcatgggtg gaggaccacc gtccagttcg tattccaccc
     2821 tggcacaccg tcatcgacga ccgttccgag ttctgtcgta tcgttatggc cgttcgaaac
     2881 catcgacgtt ggtgacccgc aacgtccggt cgctgagcag gcgggaacgc ggactgtctt
     2941 gattcccgcg ggcacgaaac gggcggaatt gcgtgtcgtg gcgacggggc atgggcaggg
     3001 aaaccgcttc aattgcgctg aattctgcag actcgagcat gaattcacgg tgaatggtac
     3061 cgcgttttcc tttgatccgt ggcgcgacga ttgctccaag aatccgattg gacccaaaca
     3121 ggcgggtaca tggaagttct ctcgggccgg ctggtgcccc ggcgcgtata ccatgcccac
     3181 catcatcgat atcaccggag cggttcagcc aggacaagaa gcgacgataa cctacgattc
     3241 catgagcccc attcacggcg aatatgaaaa tacctgtcgt cccgacgcgg gggatgcgaa
     3301 caataagtgc gtgggctgcg ccttcgacaa cagcgccgga aattgtgact tcaatggtgg
     3361 ctcacatacc ccgccccatc atcgtcttac gacgcagttg ttgctgtatc cttgatggcg
     3421 gaattggcga tcgtgcatag cttgcaaacg cacgtttatc cgcatgatat gggcaatgag
     3481 aaggtccaat atggccttta cgggacgacc actcgcagac gatgccacgg tattccactg
     3541 tgttcatctc gttggaaacc gtcggtcgcg gaatgctggt cgcggagcat gaaactataa
     3601 cgcgcgtgaa catccggggg acccaagatc gtatcccacg cggccgtact cgtatcttcc
     3661 gcataaatca aaagaccgcc cgggaccgtg ccatccaacg ccaccatccc gtaacgctgc
     3721 aaggcacgac aaatcattct cagcatccgc gtttcttccg tcacgtcgtg cgaggcattt
     3781 cttttaggaa ccgtgcgagt ttcacagtcc acggcaggat cgagttgcaa ccgcatcccc
     3841 atttccatcc aggaagcagg atcccctctt tctgctaggg aaagagggca acccttgggc
     3901 tggtcggttt tggtggccgg cggacgaaac gtatccgtaa agtggcaatt cgaataggaa
     3961 aaccgaagag catggcagat ttcaccggcg atgacctctt cgggacgaat gaggcccgcc
     4021 aaataactga gtcctgaacc cgttccccaa cctttgaacg gggttcctac actgggggaa
     4081 ccgtcgggat tcaatagctt tccatcggaa ttataatgga ataatccacc tccgctcgcc
     4141 gtgatggtct gcttcgtatg gtcgaatacc gcttgccaaa gccgcagttc cacgaaagga
     4201 ccatacgcgg ggtggttcct atccaatagc tccgagggag aatccgaccc tcccccctgc
     4261 acggcacccg ccggcacccg gaacagctcc ggtgtgggaa ccgatgatac atggaccgta
     4321 tacaacggat ccgaatcgct tacctcatag accgatggcg attccccatg ggttcccaac
     4381 cccaccgctc gcaacgtcgt attcaaagca agacgctcca ccacggcgtc gctccttgga
     4441 tccagcgtaa ccgcacttcc cagcggctgg ttgagagaaa aatgctttgg atcataaggc
     4501 agcacttccg gaggatggct cttgcaagaa ctgctttccc cagtttcttc ggcatcgagg
     4561 acatccgtga ccccatcgat cgattggtcg aacgcggtat cgtccggcag gtctatgctg
     4621 gtatctacaa ggatgtcccc ctcggtatcc atccccgaat cggtgggatt gccgtcagaa
     4681 acggacccat cgccctgcga cccctgggaa tcctccaccc cgctcgacgc atccccttgc
     4741 tttttgctag aggatgtatc ctccgactcc tgggaacagc ccatgacagc taatccgagg
     4801 agagcgccca tcacccctgg cctcgctacc acgaggatag gtcgatgatt accgataatt
     4861 cccatatccc accctatcat gccccgtgtg gcgtcgcaac cccgcataaa acgcatattc
     4921 cgggcaattc accgggcccc ggcgctcccc ccaacgtctc actccagacg cgcctgctga
     4981 cgccatggct tcctacttgg ccgcatcttt cccccccctt gtcgccattg ccgataacaa
     5041 ggataaccga ttgattttca attgaaattt gtctatgcca tgcggcgagc tttccggctg
     5101 cacccgtccc tcacaggcat tgtgcttgaa acacatcggg caagccatcc ccatcgaagt
     5161 cccctacccg aagcttgcgt acgtccaagg aactgatgcg taaacgttcc caggtttgtg
     5221 tggcgctccg cgaaacccac caggtcgagc cgtcgctacg aaggatatcg gttatcccgt
     5281 cgccatcgat atcggccaat aataaggagt ctattccata ggaagaatcg gtgagttttt
     5341 cccacgacga ggtccccgcc cacgatacgc gccatgcgct gccatcccca tgaaatacat
     5401 cggttttccc atcaccgtcg aagtccccga accccaagga gcccagcgcc acgccggagg
     5461 aggcgagcga ttgccatgtg ccttttcccc cgaaacaaac cttccattgc ccgccagagg
     5521 cggtaaatac atcggttttc ccatcgccgt cgaaatcccc gaacgctagt tggctggtcg
     5581 actccgacgc cgtgcgaagc gtttgccagg cgctcgaccc cccatacgat acatgccatt
     5641 cgctgccggt ggcgtgaaat acgtccgttt tcccatcgcc gtcgaagtcg ccgaatcgca
     5701 gggaggaaag cgtgatggtc gaggtggcga gggtagccca gagttccacg cctcccctgg
     5761 accattccca gcgcccgttt ttgctgcgaa acacatcggt tttcccatcg ccgtcgaagt
     5821 ccccgagggc cagctcttcc atcgggtcgg aagcccggct caagtacgca aaaggagcgc
     5881 ttcccccggg agcaaagcac caacgaagat gcgcacaact cgatgccgat tggccgtagc
     5941 ggttgggcgc ggcccccgac gggctttcat ccacatggaa gttgccgaaa aaatagagtt
     6001 gtcgcgcggc ttccccaggc gtggcccgtg ccaggcaatt ttcataaaac cacgccccct
     6061 gcgcaggtct accgcgaacc gcgagactat attggtccgc caccaacacg atattctcgt
     6121 gaatcagcat cttgcccccc gcgccgggat cgccgtttcc cacgcgttcg ttttccccat
     6181 gcatatcgaa tacatggccg atggagtgag gcaaaacgag attgtggcgg gacgtataat
     6241 cctgccctga tgccccgctt cccgcaatgg catgacggtt ataatcgaag cgattccatt
     6301 ccaccagcac atcgaccgca tcgcccgcgg tggcattcaa cacgacacca tagcccaggc
     6361 cccggcgctg cgtatgatga atatcgttgt gatgaacccg gtggtcagcc ccggaaccca
     6421 aattaatcgc cgcataactc caggctgtca attcattatt gtcgatctcg caacgcacat
     6481 acttgtcatt tcgaatcccg agactggggg gcgtgcaata ggcacaattg ggccccgtct
     6541 tttcacattg attgggatat tgcggtggac aggtggtcgg gtcgggcccc acgatccgaa
     6601 gccccgtgat gcgtacgtcg tcgccacata ccttgagaac cgccgtattc gtcatcgccg
     6661 tggaatacag cataccgccg gcactgcctg actccccgcg cccactggct aaccaaaccc
     6721 ccccaggaat acaaaggctc tgacccgtca gatcaatccg tgcctcgttt ctcacataga
     6781 cgatcgtacc cgctacggcg ttttccaagg cattcgacag ctcttgcagc gtggagacct
     6841 cgacggtaca cgacggattg ggcgcgagcc ggtccggata gtcggagccg ccccccccac
     6901 cgccagctag ccgattcggg aggggaagaa aggtcatggc gggttcgata ggtctggcaa
     6961 tcgaccggtg ccgcctcctc gggagcgtca aacggggcgt cgagagcatc gggcgatgcc
     7021 tctagccctt gctggggacc atcgctttgc ccactttccc agggggctga atcggttccc
     7081 gtcgcgctat cgccttgcgc cgcgtcatcg gccccttgcc ccccatcgaa tccggggccg
     7141 ggcgcgtgag cgacttcctc gcccccaccg caactcacga aacacaccat gaaactcaac
     7201 ggaagcagga gccgatgcat gatgaaccac ctgatgatgg agaaccgtcg ccacaatgcg
     7261 gtctgatcat tgataatgat gtcacgagcg catgctcgat gctgcccatg gatgcttccc
     7321 atcgccaatt agagaaataa ttgtccgctg gcagataccg cggcctggcc cgcgattttc
     7381 acgcgcaggc attcgttt
//
LOCUS       DAOSOR010000269         7395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10058_length_7395_cov_4.370300, whole genome shotgun sequence.
ACCESSION   DAOSOR010000269 DAOSOR010000000
VERSION     DAOSOR010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10058_length_7395_cov_4.370300"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5922)
                     /locus_tag="PK710_14380"
     CDS             complement(<1..5922)
                     /locus_tag="PK710_14380"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT10962.1"
                     /translation="MEQVGALTERIKQLKTIAASMHEGAFHTRLIPEIDKILALPDEL
                     SSASVGEREAVTRQVREYEQTFDRLGYTLLEQLMPSVGVSPVFQIVQAADSFKSWLRA
                     RVGQSYQRDGTSAIFDQHAFETLTRPSLSIGTPPVTAGTQRLVSRIVFGREVTGLVNH
                     IETVSSDPDSMRDLLAMVDDILCGSLHYCTAAEEAAFLRDAADFTGFEGGRILNRVVI
                     TESPDRVVELLRDHVFDKFNPGSSSPVHVVEGVDRLDAQLVAHMQAEPNHVFVVKVTG
                     IAHWLFQPPSGENIWRSVLGRLVLVDASERARRSNATMVYTLFPHVAKTLRNVQTGFA
                     GRPANTQLQLRKILERFDSTTLHTFGNDLDQRIAFLEREGVRQGSLDAMRTLEWKRDG
                     LLDYLTLRKLRRFVHLLETVSDGTDSQREHMATQMRTAVSSHWLHAFYGGLPEDRYEP
                     VVLPGGGRGALAIVGDYHFDRVQRAVDAFRSESLPECIARLKDLKQRLAIPGGSTDEI
                     EAAMKQSQMRSMSPTQRVTTDAGISLPEHLSRSVLYRAIDTARKVTARTRASIDRVTY
                     GNVTGGLASVVKHTMSQAGFGALHGHLEQLVGEHVQRADHNLRNYLLPIQDIIRAVHK
                     ATDELKGELDPIAIGDIEGMLKSIDQASFFPTLILPELSWTYGDVFPEKHFPIRWTKR
                     IPLNRRHEMDPLALLQWIEELRYLLRRFPEVFSLACESMLLVINSPHNPTGVVYRRET
                     TLRLLQIAAEYGITVVDDNAYHKVVTSKVKAREGDACAAQLYERHWHQFTQPVSLITV
                     GATTKALQGSGDRTGFVVSNMPQVIEYAQRRASEPHLMSLYLTQLKLESGLAVKRWTS
                     ALEQISGDLLSPTASQPPTQRFHSLLEQQLQQADQDDFPIPLFEELLDAYEQMLRLQR
                     RGATVTDLSRHMSSTVSRIKNLRLERALRSDVELRIKQVREAKRRAWPEAPDIVPEGA
                     FYYCIRLCPSDDDRGLLAFLRSLSAFTKVDVTYAGKGLVRLSLGGAIDGSPSSYERLG
                     RSVQVYIEILRKHWTAYDSQGRTPSALQTIFAPGNMNLDRVVADLHPLVDIYPSTSSS
                     RHGHVISRDERGTVYCIEEGRSVADKFFVEVVPCQSVRELMTSRAFHVVYRRLLRKVV
                     HRHPALADLSFERVESQYGPHACQRAYQDRQYIDDLFRTLMREMYELWHSGSSCKVLT
                     VRLEATHPTEKVAALHGIDRRLNELINELMHAFEVPDEVVSDSASFDIGYEVISSIRA
                     HRDMPDYAKTLIERCCFAVATTALNPSPNVVTGASKRVADPRYGFTRRDGDGHLAPGL
                     DHFRARLERFSRRADLRDYISKAVQVGPFQMLLQIHKSCVHLLSDELRLFPQIVEIQS
                     QQALQDVPWDGILLFGLPSKVLGDAYRTGYILDRDRAGHLLPVAWVSREDATDYVGFL
                     KKSLLTIHNERVKAMGGMPVHGAMFTITFKNGLRKTLVFSADSGTGKSETITAMMEQI
                     IAATGNASEVRRVDILASDMLSLWQGSDRQVYAFGTETGDFLRLNDITEGWKAHFGDL
                     LKRGAYSNADHPKNARVTIGGLCDVRDVLSPTRVNGFFYINNYEAPLTSAVELSADPH
                     HVLKNVLVRGLRKNKGTSGDQPSLRAGLEFAHQSPLVVRFRHDIDALIDFEERVSHGI
                     SRTFLVIHDSASDAFAARELVRSAFQGKWIKHNNQSLRIDDVSYDVLHNTFWIDTDDG
                     KLPLDRTVYDQVYEPLTSTFCGNPFMEPAGVDKILETFAELMYQAKVHTGVIRTQLAR
                     SGFEHAGPARAAEDIITFLLEDEEVNERFQRNKEKVHRAMQRTFAGVLEAGSNLPVEL
                     EGYNLLLLEEHESTHVAFLDQDDHPFTLSTPFYQVDSQDGSPMGSFIPTMTLPENGNV
                     LRDICLHRDHASRAHDFSADLSAYHGIKYWGSLEELTFQVLMINGVVGIGSTDDEVAR
                     FPIEVIKARRVAEHIA"
     gene            6275..7393
                     /locus_tag="PK710_14385"
     CDS             6275..7393
                     /locus_tag="PK710_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT10963.1"
                     /translation="MSQFREFSLDAVRTDGWFERIGQGIGSFRALCDIVGDRFFAFSM
                     ITGARITALTVDRRNPDDTLVDFVVGGEGDDGDTDAQRLPLGDFRRRLVAALLIEGPP
                     APPPMRDTDVEALQLHVGVRYLLLAPLYGYSLQSLVIENDRSMLRVLVDGRESTFDLM
                     LFQQRLRAHVRDELERVSSGQRSTIDLAKVAEAELASKEGDWRRVTKLLGTWPAPLAI
                     FLRTPEGQMIAPETSSLIAKGLGLLGSACVHLGEIERAEEIYRLGVQYGQEGPVSAEI
                     FTQFGEALLMDGRPGEAIGLLRRALALGADPKRVQPRLARAFRLRHRFVAARACLLAA
                     LDAGVDVVDVWDDIRPVREALGDAASRLPVELPSSLDS"
BASE COUNT         1564 a         1846 c         2299 g         1686 t
ORIGIN      
        1 tgcgatatgc tctgctaccc ttcgggcctt gatcacttcg atcggaaatc tcgcgacttc
       61 atcatcggtg gatccgattc ccacgactcc gttgatcatc aagacttgaa aggtgagttc
      121 ttcgaggctt ccccagtact tgatgccgtg gtaagcggaa aggtcggcgg aaaagtcatg
      181 agcgcgcgat gcatgatcgc ggtgaagaca gatgtctcga aggacattgc cgttttctgg
      241 cagtgtcatg gtggggatga acgaacccat gggcgaaccg tcttgggagt cgacctggta
      301 gaaaggggtg gacagggtga aggggtggtc gtcttgatcg agaaaggcga catgggtgct
      361 ttcatgttct tcgagcaaga gcaggttgta tccttcgagt tcgacgggca agttgcttcc
      421 ggcttcgagc accccggcga acgtgcgctg catggcgcgg tggacttttt ctttgtttcg
      481 ttggaagcgc tcgttgactt cttcgtcttc gagcaagaag gtgatgatat cttcggcggc
      541 acgggcggga ccagcgtgct cgaagcccga gcgagcgagt tgtgttcgaa tcactccggt
      601 atgtaccttg gcctggtaca taagctccgc gaaagtttcg aggatcttgt cgactcccgc
      661 gggttccatg aacggattgc cgcagaacgt gctggtcaac ggttcataga cttgatcgta
      721 aaccgtgcga tccaaaggga gtttgccgtc gtccgtatcg atccaaaagg tgttatgcag
      781 cacatcgtag ctgacatcat cgatccgaag tgattggttg ttgtgtttga tccatttgcc
      841 ttgaaaggca gaacgcacca gttcacgagc cgcaaacgca tcggatgcgc tgtcgtgaat
      901 cacgagaaac gtgcgagaaa tcccatggga aacccgttct tcgaagtcga tcagggcatc
      961 gatatcgtgg cgaaagcgga cgaccaacgg agattgatgg gcgaattcga ggccggcgcg
     1021 caaacttggt tgatcgccac tcgttccttt gtttttgcgc agaccgcgga cgagaacatt
     1081 tttgagcacg tggtgcgggt ccgcggaaag ctcgacggcg ctagtgagag gtgcttcgta
     1141 gttgttgata taaaagaaac cattgacccg tgtgggggac agcacgtctc gaacatcgca
     1201 taatccgccg atggtgacac gggcgttttt gggatggtct gcattggaat aggcgccgcg
     1261 tttgagcaga tctccgaaat gtgctttcca tccttcggta atgtcattga ggcgaagaaa
     1321 gtcaccggtt tcggtgccaa acgcataaac ttgccggtcg gaaccttgcc aaagcgacag
     1381 catatcgctg gcgagaatgt cgacacgtcg cacttcggaa gcgtttccgg tagcggcaat
     1441 gatctgctcc atcatggccg tgatggtttc ggacttcccg gttccgctat ctgctgaaaa
     1501 tacaagggtt tttctgagac catttttgaa tgtgatggtg aacatcgcac cgtggaccgg
     1561 catcccgccc atcgccttga ctcgttcgtt atgaatggtg agcaaggatt tttttagaaa
     1621 cccgacgtaa tcggtggcgt cttctctcga aacccacgcc acgggaagaa gatgaccagc
     1681 ccgatcccga tccaaaatat agcccgtgcg ataagcatcg cccaatacct tggagggcag
     1741 tccaaacagc aaaattccgt cccatggcac gtcctgcaac gcttgttggg attgaatttc
     1801 gacgatttgc gggaagagcc tgagttcgtc gctcaataga tgcacgcaag atttatgaat
     1861 ctgaaggagc atttggaaag gaccgacttg caccgctttg cttatgtagt cccgcaggtc
     1921 tgcgcgacgg ctaaagcgtt cgagtcttgc gcggaagtgg tcgaggccag gcgccaggtg
     1981 tccatcgcca tcgcggcgtg taaaaccgta gcggggatcg gctacgcgtt tggatgcgcc
     2041 cgtgacaacg ttgggactgg ggttcagggc tgtggtagcg accgcgaagc agcagcgttc
     2101 tatcagtgtt ttcgcgtagt caggcatgtc gcggtgagcg cgaatcgacg agatgacttc
     2161 gtaaccgatg tcgaacgaag cgctatcgga aacgacttca tctggcactt cgaaggcgtg
     2221 catgagttcg ttgatgagtt cgttgaggcg acggtcgatg ccatgcagcg cggcgacttt
     2281 ttccgtgggg tgagtagctt cgagtcgcac ggtaagcact ttgcacgagc ttccgctatg
     2341 ccagagttca tacatttcgc gcatcagggt gcgaaataga tcgtcgatat actgacggtc
     2401 ctgataagcc cgttggcatg catgagggcc atattgactc tcgacacgtt cgaagctcaa
     2461 gtcggcaagc gcgggatggc gatggacgac cttgcgcagc agtcttcggt agacaacgtg
     2521 gaaggctcgg ctcgtcatca gttcgcgtac ggactggcat ggaacgactt cgacaaaaaa
     2581 cttgtcggca acggaccggc cttcttcgat acagtagacg gttccgcgtt cgtcgcgaga
     2641 aatgacgtgc ccgtgtcgtg aggagctggt ggaggggtag atgtcgacaa gggggtgtaa
     2701 gtcggcaacg acgcggtcca ggttcatgtt gcccggtgca aatattgtct gcagcgcgga
     2761 gggggttcgg ccttgggagt cataggctgt ccaatgtttt cgtagaattt cgatgtacac
     2821 ctgcacggag cgaccgagac gttcgtaact cgacggggag ccatcgatgg cgccgccgag
     2881 ggaaaggcgc acaagccctt ttccggcata cgtgacgtcc actttggtaa acgcgctgag
     2941 ggaacgaaga aaggcaagca ggccgcgatc atcatccgaa gggcataagc gtatgcagta
     3001 ataaaaagcg ccttcgggga cgatatcggg agcttcgggc catgcgcggc gcttggcttc
     3061 gcgaacctgt ttgatccgaa gctccacatc tgaccgcaaa gctcgttcga gccgcagatt
     3121 tttgatccga gacacggtgc tactcatgtg gcgggacagg tcggtgaccg tggcgccgcg
     3181 acgctgaaga cgaagcatct gttcgtaggc gtcgagaagt tcttcgaaaa gagggatggg
     3241 aaagtcatcc tggtcggcct gttggagttg ttgttcgagc aacgagtgaa agcgttgtgt
     3301 aggaggttgg gaggccgtag ggctcagcaa atcaccggag atctgttcaa gcgcgctcgt
     3361 ccatcgtttc acggccaatc cggattcgag cttgagttgc gtcaagtaca aagacatcaa
     3421 atgtggttcg gaggcacgtc gttgcgcata ctcgatgact tgcggcatgt tggagaccac
     3481 aaatcccgtg cgatctccag acccttgcag tgctttggtg gtggctccca cggtgatgag
     3541 cgaaacgggt tgggtgaatt ggtgccaatg acgctcatac agttgtgcgg cacaagcatc
     3601 cccttcgcgc gctttgacct tggacgtgac cactttgtga taggcattgt catcgacgac
     3661 ggtgatgccg tattcggcgg caatctgcag caaccggagc gtggtttcac gtcgatacac
     3721 gacgcccgtg ggattgtggg gcgagttgat gaccagcagc atgctttcgc aagccaagga
     3781 aaacacttca ggaaagcgtc ggaggagata tcgcaattcc tcgatccact gcagcaatgc
     3841 aagcggatcc atctcatgcc ttcggttcaa ggggatgcgt ttggtccatc gtatgggaaa
     3901 gtgtttttcc ggaaagacgt ccccatacgt ccaagacagc tctggcagga tcaacgtggg
     3961 gaagaagctt gcttgatcga tggacttgag catgccctcg atatcgccga tggcgatggg
     4021 gtcgagttct cctttgagtt cgtcggtagc cttgtgaaca gcacggatga tgtcctggat
     4081 aggcagcaag taattgcgaa gattatggtc cgcccgctgg acgtgttcgc caacgagctg
     4141 ttccaggtgc ccgtgcagag cgccaaatcc cgcttgagac atggtatgtt tgacgacgga
     4201 agcgagtccg ccggtgacat tgccatacgt aacacggtcg atgcttgctc gcgttcgcgc
     4261 cgttaccttg cgcgccgtat caattgcacg atacaatacg gatcgggata gatgctcggg
     4321 caggctgatt ccggcgtcag tcgtcacccg ctgggtgggg gacatagagc gcatctgcga
     4381 ttgtttcatc gctgcttcga tttcgtctgt gcttcctcca ggaatggcga gacgttgttt
     4441 gagatccttc aatcgtgcga tgcattcagg caaggattcc gagcgaaagg catccacggc
     4501 gcgttgcacc cggtcgaaat gataatcccc cacgatcgcc aacgcaccac ggcctcctcc
     4561 gggcagcacg accggttcgt atcgatcttc gggaagacct ccataaaagg cgtgaagcca
     4621 gtggctcgat acggcagtgc gcatctgcgt ggccatgtgt tcgcgctggg aatcggtccc
     4681 atcactaacg gtctcgagca gatggacgaa ccggcggagc tttcggagcg tcaaatagtc
     4741 cagcaagcca tcgcgcttcc attccagcgt acgcatggca tcgagggaac cttgtcgaac
     4801 tccttctcgt tcgagaaagg cgattcgttg gtcgaggtcg ttgccaaagg tatgcagggt
     4861 ggtcgaatcg aagcgttcga gaatttttcg taactgaagc tgggtgttgg cggggcgacc
     4921 cgcgaagccc gtttggacgt ttcgcaacgt cttggcgacg tgcggaaaca acgtatacac
     4981 catcgtcgca ttcgaccggc gcgcgcgttc ggacgcgtcc acgagaacca atcgacccaa
     5041 tacgcttcgc cagatgtttt cgccggatgg tggctgaaat agccagtgcg caatacctgt
     5101 gaccttgacg acgaaaacgt gattgggctc ggcttgcatg tgggcgacga gttgtgcatc
     5161 gagacggtcg acgccttcga cgacatgcac gggagaggag cttcccggat tgaacttatc
     5221 gaaaacgtga tcgcgaagga gttcgacgac gcggtctggg ctttcggtga tgacgactcg
     5281 attgaggatg cgaccgcctt cgaagccagt gaaatcggcg gcgtctcgaa ggaaggcagc
     5341 ttcttccgcg gccgtgcaat agtgcaaact tccgcaaaga atatcgtcca ccatggccaa
     5401 taggtcgcgc atggagtcgg gatcggaaga aaccgtttcg atatgattga cgagcccggt
     5461 aacttctcgt ccgaaaacga tgcgcgacac aaggcgttgg gtaccggcgg tgactggtgg
     5521 tgttccaatg gatagacttg gacgagtcag ggtttcgaaa gcgtgttgat caaaaatcgc
     5581 ggaggtgccg tcgcgttggt agctttgtcc aacccgcgcg cgtaaccagc ttttgaatga
     5641 atcagcggct tggacgattt gaaagacagg ggatacgccc acggagggca tgagttgttc
     5701 gagcagggtg tatccgagac ggtcgaacgt ttgttcgtac tctcgaacct gtcttgtaac
     5761 ggcttctcgt tcgccgaccg aagcgctgct caactcatca ggaagagcga gaattttgtc
     5821 gatctcgggg ataaggcgtg tatgaaacgc gccttcgtgc atcgaggcgg cgattgtctt
     5881 gagctgcttg atccgttcgg tgagcgctcc tacctgttcc atggtgacca tggtatggga
     5941 aagcgcggcg aagagggcaa tccctcacga acaaacggcg gttctgtcgt ggcttttccg
     6001 ggaattgacc gcgaaaactc gcgccgtcca acgaggttgc gctgaaccaa accgcgaatt
     6061 tctgcggttt tcgtcgtcgt tcggagggac aaaactcgtc gatccgtcgg aaacataaga
     6121 ttgaaggggt aagcatactc ggaagggccg gggatagcgt gcggccgctc gagcgtaacc
     6181 ccttggatgt cggtagggtt ttcgtcgacg ggccatcacg tggcgtgaca accatctcta
     6241 tgcgtgcaat cctggggctc ttcgagcatc acccatgtcg cagttccgag aattttccct
     6301 ggatgcggtt cgcacggacg gctggttcga gcgcataggt caggggattg gcagcttccg
     6361 agcgttgtgt gacatcgtag gagatcgttt ttttgcgttc tcgatgatca cgggtgcccg
     6421 cattacggcc ctgacggtgg atagaagaaa tcccgacgac acgctggtgg attttgtcgt
     6481 tggcggtgaa ggggatgacg gcgacacgga tgcgcaacgg cttcctcttg gggattttcg
     6541 aagacggttg gtagcggcat tgttgatcga ggggcctccg gcacccccgc cgatgcgcga
     6601 tacggatgtg gaagcgcttc aacttcatgt gggtgttcgt tatctgctgt tggctcccct
     6661 gtatggttac agcctgcagt cgctcgtgat cgaaaacgat cgatcgatgt tgcgtgtatt
     6721 ggtcgatggc cgcgagagca cgttcgattt gatgttgttt caacagcgac ttcgcgcgca
     6781 cgtgcgggat gaactcgaac gggtttcctc cggacagcgt tcgaccatcg atttggcgaa
     6841 ggtagcggaa gcggaattgg cttcgaagga aggggattgg aggagggtca cgaagctatt
     6901 ggggacatgg cctgcaccgc ttgcgatttt tttgcgcacg ccggaaggac agatgatagc
     6961 cccagagacc agctcgctga tcgcaaaggg tttgggattg ctcggtagtg cttgtgttca
     7021 tcttggcgag atcgagcggg ccgaagaaat ctaccggttg ggcgttcaat atgggcaaga
     7081 aggacccgtg tcagccgaaa tattcaccca gtttggagaa gccttgttga tggatgggcg
     7141 ccctggggag gccattggac ttcttcgacg ggccttggct ctcggcgcgg atccgaaacg
     7201 agttcaaccg cggcttgcta gggcttttcg cctccgccac cgatttgtcg cggcgcgtgc
     7261 ctgccttttg gccgctctcg atgcgggagt cgacgtggtg gacgtgtggg acgatatccg
     7321 acccgtgcgg gaagcgctcg gtgatgccgc gagtcgcttg cctgtcgaat taccctcgag
     7381 cctcgattcg tagga
//
LOCUS       DAOSOR010000270         7389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10073_length_7389_cov_4.972457, whole genome shotgun sequence.
ACCESSION   DAOSOR010000270 DAOSOR010000000
VERSION     DAOSOR010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7389
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10073_length_7389_cov_4.972457"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="PK710_14390"
     CDS             complement(<1..752)
                     /locus_tag="PK710_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOT10964.1"
                     /translation="MPSSLEMSSYASGSSSAIICSEKTCSDLQWGRVLEALAQRAKGP
                     LGHRAALDLTFCATRQQVTQALGQVKEAYALLQAGKPLPTGGAPILSPILERLRAGGA
                     LTPNELLSVAQLLSKARYLQRFLQEQKSRAPLLHESCATDPQLDSLIDQIQPCFEPDG
                     SWSDRASSRLRELRQERHATRQRLLDKLHDIMQRYASTLQDDFYTERQGRFVLPVRVD
                     SHERFIGIVHGTSASGQTLFMEPRAIIALGNKL"
     gene            complement(770..988)
                     /gene="rpsU"
                     /locus_tag="PK710_14395"
     CDS             complement(770..988)
                     /gene="rpsU"
                     /locus_tag="PK710_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOT10965.1"
                     /translation="MPSEAVQCKPLEVTVGDKGIERAIKHLKRKMAAEGILRELKRRR
                     HYMKPSVKRRKKESEAARRRRKRVRAEI"
     gene            complement(1125..1979)
                     /locus_tag="PK710_14400"
     CDS             complement(1125..1979)
                     /locus_tag="PK710_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10966.1"
                     /translation="MRFNRYLRPASVVRQSILVTPSIIDPDAGLPKGPTFFFEPVYDP
                     FDRLVVFQLTARSRWVPSTLHTVRLFSPKDDGDMTGFRAFDGAPLKETESYSFMTGER
                     ESEPRDDRLPPVRYCEQDEGSDALPAVATVLRSSCGRAGCHGSSPALGLGLSTRTALQ
                     TTAVRVVARQTMTGASVSATASTPSRFGDDMPRIDPGNAANSYLVYKLLIHPQNHPGL
                     HDGDTPDPWLGGLTPSGPPSYDELSRLRSWFVHGEPMPLEGHLSAHETRAIVRWIIHG
                     APTSDCLP"
     gene            complement(2204..2458)
                     /locus_tag="PK710_14405"
     CDS             complement(2204..2458)
                     /locus_tag="PK710_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10967.1"
                     /translation="MEELTLYLLENMYLDFQGDISLETVRNFLREDDSPEARRLLTKI
                     IEENGVDEMLLTLADCLKDSISTGIRTEVIHEALNMYSDS"
     gene            complement(2579..3835)
                     /locus_tag="PK710_14410"
     CDS             complement(2579..3835)
                     /locus_tag="PK710_14410"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOT10968.1"
                     /translation="MRTSRIQVVWLGVSLLIAAGLAAMSGDRALARAGGVVAPAKQVS
                     SSVGGGSSPSELIFPARTIPIRFDHAAHHQRGATCLHCHPSAKTSDSTAKRVGWSVAA
                     CDRCHGTNHRDLAAEQPTECAFCHEGYEANEANRVRRSVVPSARLHFNHRVHAARNIG
                     CAQCHGAVQTVGKTSRDHLPRMQKCLTCHGLPDGSRGDAKAGCPTCHLTTASGRLQTS
                     FPSGKLLPPRWMGAVEHGPDFVHTHAVVAANDSRFCARCHSEADCLRCHDGRERPRQV
                     HPNDYLSMHAVAAKQSAQRCNSCHHHESFCKNCHLRAGVTLTGPGWNRAHRGRVHPPA
                     AIFVSTPITGRHHSVEARRNLPACVGCHGERDCVGCHATRGGGGIGVNPHPAGFSSRC
                     ASALARNPRPCFVCHDPDDALIFRCR"
     gene            complement(3832..5310)
                     /locus_tag="PK710_14415"
     CDS             complement(3832..5310)
                     /locus_tag="PK710_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10969.1"
                     /translation="MLRRRAPRHWATVATFCLAGSWAAAAQAFEVEVDSETTAQGYQL
                     RNAWGDPVLSRRRFLQTLGLHVVGIGENENAAASSGLDMSFHMRMRLDADFAIQPFES
                     NYADYPSAFVPGLQRAPVDLVIGYFDIRNLAGGWLSARLGRQIIVDPLGWWSLDGALL
                     RAELPVFLALEAYGGFEQRAGLPLSTGRFERDGVWRGDRTSLDANAYPEFLEAGLAPA
                     HGFVLESIDLPVVHARVAYRRVWNTGKVATSPFSEDRVGMPSTTDGMRISQERLGVSA
                     DALVEELGALRTGLIHDFPRAQITSWYATLDGFVTSWLTTGIDVDHVIPTFDGDSIWS
                     FFSTEPTTTLLGRGDVGLTNRWRLAVAGGVRWVDIESETSTTMLDVLGRASVRHDHDG
                     GRVGMGSLTDRGERGSRDGVDVFADQWFEDRFLVSGRVSVADWRDAMRTDRSTTSLGY
                     VLGGGFRVGDETMARLEWEHDTNELVGQRYRILASLQLLVSR"
     gene            complement(5345..6811)
                     /gene="pepD"
                     /locus_tag="PK710_14420"
     CDS             complement(5345..6811)
                     /gene="pepD"
                     /locus_tag="PK710_14420"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HOT10970.1"
                     /translation="MTSPLSHLEPIGLWKHFDSLRAIPRPSGDESKVIEYIEANAKRM
                     GWGVRRDAVGNICLQVPSSQGREGQPIVVLQAHLDMVCEKEVGSDFDFSRAPIDVSVH
                     GDWVSSKGTTLGADNGIGVAAALAVCEDTTVEHGPIELLFTVDEEGSLSGAKQLDPTL
                     VRGRILLNLDAENDDQIVIGCAGGCDTEIEYGARRAMAPEGHIPVKLSVGGLLGGHSG
                     LSIHENRGNAIRILARILRRYLREQEIYVERIEGGNKSTTIPREAYAVFFAPEKYLSR
                     AQQLIDEVSEAFMREIGVVDSGLRVMLERTATPSVSPFDCSSTRRLVRLLNALPHGVL
                     AMSRAHPTQVHSSTNLASVRTDVDRVTLTMSSRSMLQWSLDGVLNQIDAVSRLAGAAT
                     EETSAYGPWHVHEQSTAMALALAAFEKVYGTRPRCTVIHGGLECGPLSERLPGIDMIS
                     FGPRIENAHTPTERVSISSVQRFYSVLGDMLTRVGTHA"
     gene            6955..7269
                     /locus_tag="PK710_14425"
     CDS             6955..7269
                     /locus_tag="PK710_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10971.1"
                     /translation="MNDKSRTISRFRTGRPMVLLMGLTACSGLAKEDPQSPTPADPSL
                     AASSIVICPKDSSYDPGRNGCLAASSAPSASQAATPAFRASPPSEPVEEEPNERDMQD
                     ED"
BASE COUNT         1623 a         2243 c         2026 g         1497 t
ORIGIN      
        1 agtttgttgc ccagcgcaat gatggcccgc ggctccatga acaaggtttg accgctcgcg
       61 ctcgtgccat gcacgatacc gatgaaccgc tcgtgcgaat ccacacgcac cggcaatacg
      121 aatcgcccct gccgctccgt gtagaagtcg tcttgcaacg tcgacgcgta gcgctgcatg
      181 atgtcatgga gcttgtcgag aagacgctgc cgtgtggcat ggcgctcctg tcgaagctct
      241 cgcagacgcg acgaagcccg atcgctccag gacccatcgg gttcgaaaca gggttggatc
      301 tggtcgatga ggctgtcgag ttggggatcg gtggcgcaag actcatgcaa aagtggcgcg
      361 cgactctttt gttcctgcaa aaaacgttgt aaataccgag ccttagataa tagctgggca
      421 acgcttagca gttcgttggg cgtcagagcg ccacccgccc gaagccgctc caggatgggc
      481 gataagatgg gagccccccc cgtcggcaat ggttttcccg cctgcagtaa cgcgtacgct
      541 tccttcacct gcccaagcgc ttgcgtgact tgctgtcgag ttgcgcaaaa tgtcaggtca
      601 agagccgccc ggtgtcccaa cggacctttc gcccgctggg ccagcgcttc gagcacacga
      661 ccccactgca gatcagaaca agtcttttcc gagcagataa tggcagacga ggatcctgaa
      721 gcgtaggaag acatctcgag gctgctaggc atgagcagcc tgggtaaccc tagatctcag
      781 cacgcacgcg cttgcgacgc cttcgcgctg cttcgctctc cttcttgcgg cgtttgaccg
      841 acggcttcat ataatgacgt cgtcgcttga gttcgcgaag gataccttcc gcggccattt
      901 tgcgcttcag gtgtttgatt gcacgctcga tccccttatc accgacggtg acctcgaggg
      961 gtttacactg cactgcctcg gacggcattt tgattcgaac ctccaaaggg aaaggacgcg
     1021 ggaccatgcc agaggaaagc gtggtgggca agcctcgtga gcgatggggg gtattttttt
     1081 gcggctgacg gggggctttg atacgttttc gtcgtcggcc gccgtcaggg aaggcaatcg
     1141 gaggtaggcg ctccgtggat aatccatcgg acgatcgccc gcgtttcgtg ggccgatagg
     1201 tggccttcca agggcattgg ttcaccgtga acgaaccagg aacgcaatcg agaaagttcg
     1261 tcgtaagagg ggggaccaga aggtgtcagc cccccaagcc acggatcggg ggtatcgcca
     1321 tcgtgcagcc ccggatggtt ttggggatga atcaaaagct tatacaccaa gtagctgttt
     1381 gctgcgttcc cggggtcgat ccgcggcata tcgtcgccaa atcggcttgg tgtgctcgct
     1441 gtcgccgaca ccgaggcgcc cgtcatggtt tggcgcgcca cgacgcgaac ggctgtcgtt
     1501 tgcaaagcgg ttcgcgtgga aagccccaac cccagggcgg gagaagaacc gtggcatccc
     1561 gcacgcccac acgatgaccg caataccgtg gccacggcag gtaacgcatc actgccttcg
     1621 tcctgttcac aatagcgaac gggcggcaat cgatcgtcgc gaggctctga ttcacgctcg
     1681 cccgtcatga aagaatagct ttccgtttct ttcaggggcg cgccatcaaa agctcgaaag
     1741 cccgtcatgt cgccgtcatc tttgggagaa aaaagtcgaa ccgtatgcaa ggtggaaggg
     1801 acccaccgag aacgagcggt caattgaaag accacgagcc ggtcgaaggg gtcgtagaca
     1861 ggctcaaaaa aaaatgtggg acctttggga agcccagcgt ccgggtcgat gatgctgggg
     1921 gtgacaagga tggattggcg tacgacgctt gcaggccgca ggtatcggtt gaagcgcaag
     1981 cgaagggccc gttcgagggg aaaggacgat tcggagggct gaggttcgga ttcgaggacg
     2041 agcaattcag gcgggtcgaa acgcgagtcc ggtgcgtcgc gatcgcaagc aaaaagcgcg
     2101 gcgatgaggc atgcgatgaa tagatgtgtg gaaataggaa cggtgaataa ccgttttttc
     2161 gtggttcgaa cgcgatgggc gctcgatggc gaagctggtt ggctcaggaa tccgaataca
     2221 tattcaaggc ttcgtggatg acttccgttc gaattccggt ggaaatactg tctttcaagc
     2281 aatccgccag ggtgagcaac atctcgtcca ccccgttttc ctctatgatt ttcgtcaaaa
     2341 gccgcctcgc ttccggactg tcgtcctcgc gtagaaagtt tcggaccgtc tcgagtgaaa
     2401 tgtcgccctg gaagtcgaga tacatattct ccagaaggta cagcgttagc tcctccacag
     2461 gcgtcctcct tgcacaacat tgcgctgggt aacctcaggc atggttcgct atacccgccc
     2521 gaccgggtgg tcggcaagga gtaatcgcgg cttgctgccc cgggcgttgc cttattgctc
     2581 atcgacagcg gaaaataagc gcatcgtcag ggtcgtggca aacgaaacaa gggcgaggat
     2641 ttcttgccaa cgccgatgcg catcgagaag aaaaaccggc aggatgcgga ttgaccccaa
     2701 ttcctccacc acctcgcgtg gcatgacatc ctacacaatc gcgttcccca tgacagccga
     2761 cacaagcagg taagtttcgt cttgcttcca ccgaatgatg ccgaccggtg atcggagtcg
     2821 aaacgaatat cgccgctggt ggatgaaccc ttcctcgatg ggcccgattc catccggggc
     2881 cggtgagggt tactcccgca cgcaggtgac agtttttgca aaatgactcg tgatgatggc
     2941 acgagttgca acgctgcgcg ctttgcttcg ccgctaccgc atgcatcgat aaataatcat
     3001 tcggatgcac ctgccgcgga cgctctcgtc catcatggca acgaaggcaa tccgcttcgc
     3061 tatgacaccg cgcacaaaaa cgcgaatcgt ttgccgccac caccgcatgg gtatgaacga
     3121 aatccggacc atgctcgacc gcgcccatcc accgcggtgg cagcagtttt cctgaaggaa
     3181 acgaggtttg aagacgtccg gatgcggttg tcaaatggca cgtggggcaa ccggccttcg
     3241 catccccacg ggatccgtcg ggaaggccgt ggcaagtcaa gcatttttgc atgcgtggca
     3301 gatgatcacg ggatgttttc cccacggttt gcaccgcccc atgacattgc gcacaaccga
     3361 tatttctcgc cgcatggacg cgatgattga agtgcaaccg agccgaggga accaccgatc
     3421 gccgtacccg attcgcttcg tttgcttcat aaccttcatg gcaaaaagcg cattccgtgg
     3481 gctgttccgc cgccaggtcc cggtgattcg tcccatggca tcggtcacac gccgccaccg
     3541 accagcctac ccgcttcgct gtgctgtcac tcgttttggc ggaaggatgg caatgaagac
     3601 aggtagcccc ccgctgatgg tgcgccgcat gatcgaaacg aatgggaatc gttcgagcag
     3661 gaaagatcaa ttcactcgga ctgcttccgc cgccgaccga cgaggaaacc tgcttcgcgg
     3721 gggctacaac cccaccggca cgggccaacg ctctatcgcc ggacatcgcc gccaagcccg
     3781 ccgcaatcag caaactcacg cccagccata caacctggat tcgactcgtt ctcaccggct
     3841 caccagcaat tgcaacgagg ccagaatgcg atatcgctga cctaccaact cgttcgtgtc
     3901 atgctcccat tcgaggcgcg ccatcgtttc atcccccact cgaaaaccac ccccaagcac
     3961 atagcccaag gacgtcgtcg aacgatccgt acgcatcgca tcacgccaat ccgccacact
     4021 cacccgtccc gataccaaaa accgatcctc gaaccactga tccgcaaaaa catcgacccc
     4081 atcgcggctg ccgcgctccc ctcggtccgt aagacttccc attcctactc gcccaccgtc
     4141 gtgatcatga cgcaccgagg cccgacccag cacatcgagc atcgtggtag acgtttcgct
     4201 ttcgatatcc acccaacgaa ctccacccgc gaccgccagc cgccaacgat tcgtcagccc
     4261 cacatcaccg cgccccaaca aggtcgtcgt tggttccgtc gaaaaaaaac tccagatcga
     4321 atcaccatcg aacgtgggaa tcacgtggtc gacgtcgatg ccggtcgtta gccatgaggt
     4381 aacgaatcca tcgagcgtgg cgtaccaaga tgtgatttgc gctctcggga agtcgtggat
     4441 caatccggtt cgcaaggctc ccagctcttc gacaagggcg tcggcggata caccgagacg
     4501 ctcctgggag atgcgcattc catcggtggt cgaaggcatt cccacgcgat cttcagaaaa
     4561 cgggctggtg gcgactttgc ctgtattcca tacgcgacga tacgccacgc gagcgtgaac
     4621 gaccggcagg tcgatgcttt cgagcacgaa tccgtgggcg ggagccaatc ctgcctcgag
     4681 aaattcagga tacgcattgg catcgaggga ggttcgatcg ccgcgccata cgccgtctct
     4741 ttcgaatcga cccgtggaaa ggggtagccc cgcacgttgt tcaaagcctc cgtaagcttc
     4801 gagggcgaga aataccggta gttcggcgcg aagcagcgca ccatccaacg accaccaccc
     4861 cagcggatcg acgatgattt gtcgtcctag cctggcgctc aaccagcctc ccgcgaggtt
     4921 gcgaatatcg aagtagccga tcaccagatc gaccggcgcc cgttgcagtc ccggaacgaa
     4981 agccgatggg taatccgcgt agttgctttc gaacggttgg atcgcgaagt ccgcatcgag
     5041 gcgcattcgc atgtgaaacg acatgtcgag gcccgaggaa gcagcggcat tttcgttttc
     5101 gccaatgccc acgacatgca agccaagggt ttgcaaaaac cgacgacggg acaggaccgg
     5161 gtccccccac gcattgcgaa gttgatagcc ttgcgccgtc gtttcagaat cgacttcgac
     5221 ttcaaaagcc tgggcggcag cagcccacga gcctgccaag cagaaagtcg ctacggtcgc
     5281 ccaatgccgc ggggcgcgtc gtcgaagcat ggctgtcgca caatccagcg gaccggcaga
     5341 ggtgtcaagc gtgggtgcca acacgggtca gcatatcgcc caggaccgaa taaaaacgct
     5401 gaaccgatga aatactcacg cgttctgtgg gtgtatgtgc gttctcgatc cggggaccaa
     5461 aagaaatcat gtcgattccc ggcaatcgct cactcaaagg accgcattcg agcccaccat
     5521 gaatgaccgt gcagcgaggc ctggtcccat acaccttctc aaacgccgca agcgcaaggg
     5581 ccatcgccgt ggattgttca tgcacatgcc agggtccgta cgccgacgtc tcctccgtgg
     5641 ccgctcccgc aagcctggat accgcatcga tctgattcaa tacgccatcg agactccatt
     5701 gcagcatcga acgactactc atcgtcaagg tgacacgatc cacgtccgtc cgaaccgacg
     5761 caagattcgt ggaactatgc acctgggttg gatgggctcg actcatggca agcacaccgt
     5821 gaggcaatgc gttgagcaat cgaacgagtc gtcgcgtgga tgagcaatcg aaaggggaaa
     5881 cggaaggggt ggcggtacgt tcgagcatga cccgcagacc ggaatcgacc accccgattt
     5941 cccgcatgaa ggcttcgctc acttcatcga tcaattgttg agcgcgcgac aaatattttt
     6001 ctggggcaaa aaataccgca tacgcctctc gcggtatcgt cgtcgactta tttcccccct
     6061 cgattctctc cacgtaaatc tcttgttcgc gcaggtaacg gcgcaaaatt cgcgcaagaa
     6121 ttcggatggc gtttcctcgg ttttcatgga tcgaaagacc cgaatgtccc ccaagcagcc
     6181 cccccacact cagtttgacg gggatatgcc cttctggggc catggcccga cgcgccccat
     6241 attcgatttc cgtatcgcat cccccagcgc atccgatcac gatctgatca tcattttccg
     6301 catctaaatt caataagata cgtccgcgaa ccaacgtggg atcgagctgt ttcgcgccgc
     6361 tcaaactccc ttcctcgtcg accgtgaata gcaactcgat gggtccatgc tcgaccgtcg
     6421 tatcttcaca caccgcaaga gctgccgcga cgccaatgcc gttgtcggcg ccgagcgtgg
     6481 ttcccttgga cgatacccaa tcaccatgga ccgatacatc gatgggagcg cgactgaagt
     6541 cgaaatcact gcccacttcc ttttcgcaca ccatatcgag atgcgcttgc aaaaccacga
     6601 taggttgccc ctcccggcct tggctcgatg gtacttgcaa acaaatattg cctaccgcat
     6661 cccgtcgcac cccccaaccc atgcgtttgg cattcgcctc aatatattcg atcaccttgc
     6721 tttcatcgcc agaaggccgg ggaatggctc gaagcgaatc gaaatgtttc cacagaccga
     6781 tgggttccaa gtggctcagc gggctcgtca cgggttctct ttcctttcgc gatgggttct
     6841 ccaccctctg ttgccggaat tgtcacgacg atggatcgga agcaagcttt gtcgctgcct
     6901 gcccttgacg aattcgttga ccctgccgtg ttcccattcc agtctttgcc cacgatgaac
     6961 gataagtcac ggaccatttc caggtttcgt accggcaggc cgatggtact tctgatggga
     7021 ttgaccgctt gttctggcct ggcgaaagaa gatccgcaat cgccgacccc cgccgacccc
     7081 agcctcgcgg cttcgagtat cgtcatctgc cccaaggata gctcctacga tcccggccgt
     7141 aacggttgct tggcggcctc ttctgcgcct tcggcgtccc aagcagccac ccccgctttc
     7201 cgcgcctcgc ctccctctga gccggtagaa gaagaaccga acgagcgaga catgcaagat
     7261 gaagactgat ttcaccgttt tctgcgcatc ccctattgct agttgctagt tgctagttgc
     7321 tagttcctag ttccaactag tactacagcc acgaagcacg ttccgcttat ttatggaaat
     7381 aaattggta
//
LOCUS       DAOSOR010000271         7385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10079_length_7385_cov_4.109141, whole genome shotgun sequence.
ACCESSION   DAOSOR010000271 DAOSOR010000000
VERSION     DAOSOR010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7385
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10079_length_7385_cov_4.109141"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PK710_14430"
     CDS             complement(<1..742)
                     /locus_tag="PK710_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10972.1"
                     /translation="MAASDPALPFPTVPWLWHSGWVLARHFPVFFVLFGSLLAGCDDK
                     PPKPSWPPPPPARTDLQEDASLSKIFGIDASETPVDPPAPAGDFKRELAAFTSLDACV
                     ADRAPFDSVVGDAIDALGYDTLQRDSCRVLQAAREKNAALCEPILSSSLRHHCQSTVA
                     VTLANPLLCPMVGANHDPLCIALARRDDRLCVFVASTDRVTCRAILSREPSACGQDAR
                     CARKAQRWKTFLPDNIERPQLGTRATVRM"
     gene            877..1971
                     /gene="recA"
                     /locus_tag="PK710_14435"
     CDS             877..1971
                     /gene="recA"
                     /locus_tag="PK710_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOT10973.1"
                     /translation="MLDQKDRQKALEVAIAGVEKEFGKGAIMRLQDGQTLQPDLPVVS
                     TGSIGLDVALGIGGYPRGRIVEIFGPESSGKTSLTLHAIASAQAQGGVAAFIDAEHAL
                     DLNYAKRLGVKTDELLISQPDFGEQALEIADMLVRSSAVDVIVVDSVAALVPKAEIEG
                     DMGDSHMGVQARLMSQALRKLTGTVARSQTLLMFINQIRMKIGVTFGSPETTTGGNAL
                     KFYSSVRLDIRRIGSIKETSPDRREPVVVGNRTRVKVVKNKMAPPFREVEFDILYGKG
                     INQIGDLVDLGVEEQIVEKSGAWYSYGGERLGQGREAAIKFISEHPEIQSKIRSQVLG
                     KHGLERRIQTEEPSKVATAEEDKTKKTKRS"
     gene            2136..2627
                     /gene="sixA"
                     /locus_tag="PK710_14440"
     CDS             2136..2627
                     /gene="sixA"
                     /locus_tag="PK710_14440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HOT10974.1"
                     /translation="MMKLFMMRHGTAEDADHVGEVQDFDRALSMRGRKRIRQMGRALR
                     ENWLLPDLIASSPLVRALQTAEILAASLDPNEPVIVRRELAPQGDIFALVKELMDSEI
                     ESALLVGHEPSLSEFVTSLLGDESWGGGFTKGMIVALRVGRDGVAKALFLMDTKRLEP
                     IPL"
     gene            2759..4390
                     /gene="pruA"
                     /locus_tag="PK710_14445"
     CDS             2759..4390
                     /gene="pruA"
                     /locus_tag="PK710_14445"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOT10975.1"
                     /translation="MLDAMMLPPPPVNEPVTAFEPGSKAREQLKAELARLSSSPVEFG
                     HFIAGREVRTGKIVEVHAPHNHSLLLAKRFKGDESEVKLAIDAALKAKHDWARMPFSA
                     RAAIFLKAADLLATKYRNLTNATTMLGQSKTAHQAEIDSACELIDFWRFNVHFAARLM
                     QDQPLSSPGTWNISEQRPLDGFVLAITPFNFTSIAGNLPTAPAIMGNTVVWKPAETAV
                     LSAWAIMQCLNEAGLPAGVVNLVMGPGRVVGPLCLPHPQLAGVHFTGSTAVFQDIWRT
                     IGNNIANYKSYPRIVGETGGKDFIFVHPTADVAAVATAIVRGGFEFQGQKCSAASRVY
                     APKSLWSEIKDRVIAMVREIGVGDVTDFRNFMGAVIDAAAFRDHKAYIDLANSDSSCT
                     VLTGGTYDDKVGYFIQPTFIETSNPKHKLMQEEIFGPVVTAWVYDDAKVDEALDLLDD
                     TSPYALTGAVFAQDRAAIEYVMQRLRFSAGNFYINDKPTGAVVGQQPFGGSRASGTND
                     KAGSLLNMMRWASPRSIKETFVPPTSHRYPFMDAE"
     gene            complement(4589..5179)
                     /locus_tag="PK710_14450"
     CDS             complement(4589..5179)
                     /locus_tag="PK710_14450"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HOT10976.1"
                     /translation="MRSTFLLISLLTLPLACTPSQSPPSEPGAPSPAEHGTAQPTPSE
                     EPQQPSSASPEPAAEPASGSLHLPASTASAWNTGQSDGQPGKADRGIADYQKIIQNDR
                     EKFRSCYDAALAKRKDIKGRVTLVWVLDPAGAVRTADDHKPHIDPNASDFYDEFLEAC
                     MVGAVKTLTFPPSRRGMDSTVRYPFDFQPQGRPPSR"
     gene            5335..6669
                     /locus_tag="PK710_14455"
     CDS             5335..6669
                     /locus_tag="PK710_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10977.1"
                     /translation="MNTHIVLTSMAHRGGWFRRLSVAWLVVGLIACAACQPAKDPSSL
                     RSPHAQKWFDRARNSFQQMDVEDAQDAIGQALRLAPQDPEIRLLAANLAIAKLDYAEV
                     LRLTEGVSDSKALAIRGRAQWYLGDIQATSATLEALLRDPDTHDPWAKAISKLANHGT
                     GRKPYRMEGDIVASVEMPRVRGTAMVVPVEIDGEQALALLSTGTAEVVLDKTQRAEPS
                     WISVRIDGRVEFQDVPAVTQDLSGVSRQLGAPIKAMFGVNFLRNANVTFDFQGRQFVV
                     RQFSPPRPPRATDIPVHYIRGGGMMVRSALASDGSASLYVDSSQMFPLALDTDGWRKA
                     GVDPKTLTPFEGDPKLRHGIVPLVRLGAFDIPQVAAVLSNEIEGLEQVLGLNVDGVVG
                     SGLLGGFRVTLGDSGKVLWVEEDLVTESGLNPAPSPKPADDDTANPPVAPGP"
     gene            6685..7197
                     /gene="coaD"
                     /locus_tag="PK710_14460"
     CDS             6685..7197
                     /gene="coaD"
                     /locus_tag="PK710_14460"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOT10978.1"
                     /translation="MVNDKSKPNAGKLVVYGGSFDPVTIGHLDIIHRAVSLFDRVVVA
                     IGQHPTRQPFFSVEERLALLRGVTADLPSVEVTSFEGLLVDFCLQRKADAIVRGLRAV
                     TDFENELQIAHANADLEPCIDTIFLPTRTNYGFISASLVREIASHGGDVSRYAPPLVC
                     EALARKYASR"
BASE COUNT         1532 a         2057 c         2224 g         1572 t
ORIGIN      
        1 ccatgcgaac agtggctcgg gtgcccagtt gcggacgctc gatgttgtcg gggagaaacg
       61 tcttccatcg ctgcgctttg cgtgcgcacc gggcgtcttg tccgcacgcc gatggctccc
      121 gcgataaaat ggcgcgacac gtgacccgat cggtcgatgc cacgaacaca caaagccgat
      181 cgtcgcgtcg ggcgagcgcg atacacaacg gatcgtgatt ggcgcctacc atcggacaaa
      241 gcaagggatt ggcgagggtg acagcgacgg tgctttggca atgatgtcgt aaggaagaag
      301 agaggatggg ttcacataag gcagcgtttt tctcgcgggc ggcttgcagt acgcgacagg
      361 agtctcgctg gagcgtatcg taaccgagag cgtcgatggc atcacccacc accgagtcga
      421 agggggcgcg atcggccacg caagcatcca gggacgtgaa agcggctagt tcgcgtttga
      481 agtccccggc cggagcaggg ggatcgaccg gcgtttcgct ggcgtcgata ccaaaaatct
      541 tcgaaagact cgcatcctct tgcaggtccg tccgggctgg gggaggaggc ggccaggaag
      601 gtttcggggg tttgtcgtcg cagcctgcca gcagcgaccc aaatagcacg aagaaaacag
      661 gaaaatgacg agctagcacc catccactgt gccacagcca gggcaccgtg gggaaaggga
      721 gcgcggggtc ggacgctgcc attattcttg ccatctgaac aaaagagcag tatttaccca
      781 tacgatgagc aagatatgag gctcgtgcag cgacggcccc gaggctgttg cgcttcaaga
      841 ccgtggacag gatccacgtg cgtggaggaa atgatcatgc tcgaccaaaa agaccggcaa
      901 aaggcgctgg aagtcgcgat cgcaggcgtg gaaaaagaat ttggcaaggg cgcgatcatg
      961 cggttgcaag acggtcaaac gcttcaacct gatttgcctg tggtgtctac aggttcgatc
     1021 ggtttggatg ttgctctggg aattggtggc tacccccgag gccggatcgt tgagattttc
     1081 gggccggagt cgagtggaaa aacctcgctc acgctgcatg ccatcgcgag cgcccaagcg
     1141 cagggggggg tggccgcctt catcgatgcg gaacatgccc tcgacctgaa ttatgcaaaa
     1201 cggctgggcg tgaaaaccga cgaactactg atcagccagc cggacttcgg cgaacaagct
     1261 ttggaaatcg ccgatatgct ggtccgctcc agtgccgtgg acgtcatcgt cgtggattcg
     1321 gtcgccgctc tcgttccgaa agcggagatt gaaggggata tgggcgattc ccatatgggc
     1381 gttcaggccc ggttgatgag tcaagcgttg cgaaagctca caggcacggt ggcaagatcg
     1441 caaaccctac tcatgttcat caaccagatc aggatgaaaa tcggagtgac ttttggcagt
     1501 cccgaaacga ccaccggcgg caacgcgctg aagttttact cgagcgtgcg gctcgatatt
     1561 cgtcgcattg gctcgatcaa agaaacctcc cccgatcgaa gagagcccgt ggtcgtgggc
     1621 aaccgcacca gggtcaaggt cgtgaaaaat aagatggctc cccctttccg ggaggtggaa
     1681 ttcgacatcc tttacggcaa gggaatcaat caaatcggcg acctcgtgga cctcggtgtc
     1741 gaagagcaga tcgtcgagaa aagtggtgcc tggtactcct acggtgggga gcgactcgga
     1801 caaggtaggg aagccgccat caagttcatt tcggaacatc ccgaaattca atcgaaaatt
     1861 cgtagtcagg tcctgggcaa gcatggtctc gagcgacgga tacagaccga ggagccttcc
     1921 aaggttgcca ccgccgaaga ggacaaaacc aaaaagacca aacggtcatg aaaggcgtgt
     1981 gagcgaatca tcgattcacg gtaccatccg gtcgttgggt ttgctggcaa ctgcggtggt
     2041 acgaatcatc gtagtgattc gctttgggct ttttgcccgg tcatgggatt tggtatctgc
     2101 gctgacgctc accctttctt ttcttaggat ctccaatgat gaaactgttt atgatgcggc
     2161 atggaactgc tgaagacgcg gaccacgtgg gggaggttca ggacttcgac cgggcgttga
     2221 gcatgagagg gcgcaagcgg atacgccaga tgggtcgggc ccttcgtgaa aactggcttt
     2281 tgcccgacct catcgcttcc agtcctctgg tgcgggcgct gcaaaccgcg gagatcctcg
     2341 cggcctcgtt ggatccaaac gaaccggtga tcgttcgccg agaactggcg ccgcaggggg
     2401 atatctttgc tcttgtcaag gagttgatgg actcggagat cgagtctgcc ttgctggtcg
     2461 gccatgaacc gtctctttct gaattcgtga cctcgctttt gggcgacgag tcttggggtg
     2521 gaggtttcac gaaaggaatg attgtggcgc tccgtgtggg aagagacggc gttgccaaag
     2581 ctttattttt gatggatacc aaacggttgg agccaatacc cctgtgaaga caggggggac
     2641 gcatggtggg tgtggttgcg gatgattccc ttggctctca ccgcacggca gggtaggctg
     2701 gctgtgggtc gaggtagcag cctccccata accgtaatta cctccccagg agcaagtcat
     2761 gctggatgcg atgatgcttc ccccgccgcc ggtcaatgaa cctgtcacag ctttcgaacc
     2821 cgggagcaag gcccgtgaac aactcaaggc cgagcttgcg cgactgtcgt cttccccggt
     2881 cgagttcggt cacttcatcg caggacgtga agtgcgaacg ggcaagatcg tggaggtgca
     2941 tgcaccccac aaccattcgt tgcttcttgc caaacgattc aaaggcgatg agtcggaagt
     3001 caagcttgcg atcgatgccg cattgaaggc aaagcatgat tgggcgcgca tgcccttttc
     3061 ggcccgtgcg gcgatctttc tcaaggccgc ggatttgttg gcaaccaagt accgcaacct
     3121 gaccaatgcc accacgatgc tcggccaaag caaaacggcc catcaagcgg aaatcgattc
     3181 ggcttgcgaa ctcatcgatt tctggcgctt caacgtccat ttcgccgctc gtttgatgca
     3241 agaccagccc ctgtcgagtc ccggaacctg gaacatctcc gaacagcgtc cgctcgatgg
     3301 cttcgtgctt gccatcacac ccttcaactt cacctccatc gcgggcaact tgccaaccgc
     3361 tcccgcgatc atgggcaata cggtggtgtg gaaaccagcg gaaacagccg ttctttccgc
     3421 ctgggccatc atgcaatgcc tgaacgaagc gggcttgccc gcaggggtcg tcaatttggt
     3481 catggggccc gggcgagtgg ttggacctct atgtctgcct catccacagc tcgcgggtgt
     3541 ccacttcacc ggttccaccg ccgtgttcca ggatatttgg aggacgatcg gcaacaacat
     3601 cgcgaactac aaaagctacc cgcgtatcgt cggcgaaacc ggcggcaagg acttcatttt
     3661 cgttcatccc acggctgatg tggcagcggt ggccacggcc atcgttcgcg gtggtttcga
     3721 attccaagga cagaaatgct ccgcggcatc gcgggtctac gcaccgaaat ctctgtggtc
     3781 cgagatcaaa gaccgcgtga ttgccatggt tcgtgagatc ggggtgggcg atgtgaccga
     3841 cttccgcaac ttcatgggcg cagtcatcga cgctgctgcc ttccgcgatc acaaggccta
     3901 tatcgacctc gccaattccg actcttcgtg taccgttctg acgggcggca cctacgatga
     3961 caaggtggga tacttcattc agcccacgtt catcgaaacg tccaacccca agcacaaact
     4021 catgcaagag gaaatcttcg ggcccgtggt gacagcgtgg gtgtacgatg atgccaaggt
     4081 cgatgaggcg ttggacctgc tcgacgatac gagcccgtac gcgttgacgg gtgcggtatt
     4141 tgcccaggat cgcgccgcca tcgaatacgt gatgcagcga ttgcgatttt ccgccggtaa
     4201 cttctacatc aacgacaaac ccaccggtgc ggtggtgggc caacagcctt tcggaggaag
     4261 tcgcgcctcc ggaaccaatg acaaggcagg aagcttgctc aacatgatgc gatgggcttc
     4321 cccgcgatcc atcaaagaaa ccttcgttcc gcctacctcg catcgctatc cgtttatgga
     4381 tgcagaataa cggcaatacc gcgttattcc acaataaatc gtcttagcta gatcgttgta
     4441 tccctgtaca ttcaaggatt catggtgcct tcgttcgcgc cctccccatc cgttacgcct
     4501 ttcgctcgtg caatccctat ccgtaccagg gatgtggctt cgaacggtgg cgttcgtaca
     4561 agacggccgt aggggccagc ccttgggctc aacggcttgg aggacggccc tgcggctgaa
     4621 aatcgaaggg gtaccgaacc gtcgagtcca tgcctcgtcg cgaaggcggg aaggtcaacg
     4681 ttttgaccgc gcccaccatg catgcttcga ggaactcgtc gtagaaatcc gaggcgttcg
     4741 gatcgatatg gggcttgtga tcatcggcgg ttcgaacggc gccggcgggg tcgaggaccc
     4801 ataccaacgt tacccgtcct ttgatgtctt ttcgtttggc caaggcggca tcgtaacagc
     4861 ttcgaaactt ctcccggtcg ttctggatga tcttttggta atcggcgatg ccccgatccg
     4921 cttttcctgg ttggccatcg ctctgtccgg tgttccatgc cgaagcggtg gaagcaggga
     4981 ggtggagcga accggaagcc ggttccgcgg ccggttcggg ggatgcggac gatggctgtt
     5041 gcggttcctc ggacggcgta ggttgcgccg tgccgtgttc ggcggggctg ggggcgccgg
     5101 gttcggaagg gggagattgg gagggggtac aagccagcgg tagtgtcaac aggctgatca
     5161 gtaaaaaagt ggaacgcatt gctgaagact acgacgattc ttggtcgttg gtcgagaagc
     5221 ttggcggtcc gttctcgacc tgcatcccga tatcggctat ctcctggcct cggcccgctt
     5281 ccgtgggttc gggatatatc gtctcgacac ggtacgcgcg ctgctcagat tcccgtgaac
     5341 acccatatcg ttttgacttc tatggcgcac cgtggtggtt ggttccgacg cctttcggtc
     5401 gcatggctcg tcgttggtct tatcgcatgt gccgcgtgcc aaccggccaa ggaccccagt
     5461 tcgcttcggt cgccccacgc ccaaaagtgg ttcgatcgag cgcgaaactc tttccaacaa
     5521 atggacgtgg aggatgccca agacgccatc ggtcaagcgt tacgtctggc cccccaggat
     5581 cccgaaatcc gcttgttggc agccaatctc gcgatcgcca agctcgatta tgcagaggtt
     5641 ctacggttga ccgagggggt ttcggactcc aaggcgcttg ccatccgggg acgcgcacaa
     5701 tggtatctcg gcgacatcca agcgaccagc gctaccctcg aagcgctgct gcgcgacccg
     5761 gacactcacg atccttgggc aaaagctatc tccaaactcg cgaaccacgg aacaggacgc
     5821 aaaccctatc gcatggaagg ggatattgtg gcgtcggtgg agatgccgcg cgtgcgtgga
     5881 acggcgatgg tcgtgcctgt cgaaatcgat ggtgaacagg cgctcgcgct gctgtccacg
     5941 ggcaccgccg aggtggtgtt ggacaaaacc cagcgagcgg agccctcctg gatttccgtg
     6001 cggatcgacg gacgagtgga gtttcaagat gttcccgcgg tgacgcaaga cctgtccggg
     6061 gtttcccgcc aactcggagc gccgatcaaa gccatgttcg gcgtcaactt ccttcgtaac
     6121 gccaatgtca ccttcgactt ccaaggccgt caattcgtcg ttcgtcagtt ttctcctccg
     6181 cgtccgcccc gtgccaccga catcccggtt cattatatcc gtggtggagg aatgatggtg
     6241 cgaagcgctt tggcatctga cggctccgcc tctctttatg tcgattcatc gcaaatgttt
     6301 ccgttggccc tcgataccga cggatggcgc aaagctgggg tggaccccaa aacgcttacc
     6361 cccttcgagg gagacccaaa acttcgccat ggcattgtcc cgctggtgcg cctgggtgct
     6421 ttcgatatcc cgcaggtggc ggcagtatta agtaatgaaa tcgaagggtt agagcaagtt
     6481 ctgggcctga atgtcgacgg ggtggtcggc tccggattgc tcgggggatt tcgtgtgacc
     6541 cttggagatt ccggcaaagt cctatgggtc gaagaagacc tcgtgactga gtccggactg
     6601 aatccggcac catccccaaa acccgccgat gacgacactg caaacccgcc cgtggcaccg
     6661 ggcccctgag gggtggaagg tccgatggtt aacgacaaga gcaaaccgaa cgctggcaaa
     6721 ctcgttgtct atggtggaag tttcgatccc gtgaccatcg gtcatttgga tatcattcac
     6781 cgtgctgtca gcttgttcga ccgagtcgtg gtggcgatag gccagcatcc cacgcgtcaa
     6841 ccattcttta gcgtggaaga acgtcttgct ttgcttcgcg gagtgaccgc cgatcttccc
     6901 agcgtggagg tgacttcttt cgaaggattg ttggtcgact tttgtttgca gcgcaaagcc
     6961 gatgccattg tccgggggct gcgggcggtc acggacttcg aaaacgagtt gcaaatcgcg
     7021 catgccaacg ccgacctcga gccatgcatc gacaccatct ttctaccgac acgaaccaat
     7081 tatggcttca tctccgcgtc gctggtccgt gagatcgcga gccatggtgg cgacgtttcc
     7141 aggtatgcac cgcccttggt ttgcgaggca ttggcccgca aatacgcgtc gcgctaagta
     7201 ccccttcttt tcattgggca aacagcgaga ccgtaaagcc tcgtggcgtg tacgggtgat
     7261 tgtcaggatc gaaagcgata tcttgcagca cggagccggt gttggtggct acgacgagaa
     7321 tgcctttggc gcctgtcaac gtttcgatgt tggcttcgac gctagtgcct cgttcttcgg
     7381 tcata
//
LOCUS       DAOSOR010000272         7377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10093_length_7377_cov_4.246790, whole genome shotgun sequence.
ACCESSION   DAOSOR010000272 DAOSOR010000000
VERSION     DAOSOR010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7377
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10093_length_7377_cov_4.246790"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            333..2363
                     /locus_tag="PK710_14465"
     CDS             333..2363
                     /locus_tag="PK710_14465"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HOT10979.1"
                     /translation="MSNPYPHLLAPLDLGFVTLRNRVLMGSMHTGLEDSIWNYGKLAA
                     YLGARARGGVALIVTGGYAPNLSGRVAPLAGKLTHRFETIKHRPMTRAVHDEGGRICL
                     QILHAGRYAFHPLSVAPSAIRSPISPFKPRALSDRQIRKTISDFVRCAQLAREAGYDG
                     VEIMGSEGYLINQFLAERTNQRTDAWGGTFDHRTRFAVEIVRQTREALGPDFIVIFRL
                     SMLDLVPRASDWQQVVTLAKQIERAGATMINTGIGWHESRVPTIATVVPRAAFTWVTR
                     KLKTEVGIPLITSNRINAPDVAEEALARGDADMVSMARALLADPDFVRKAEQGRADEI
                     NTCIACNQACLDHVFANRRASCLVNPRACRETEIRLHPVRRRKRVAVIGAGPAGLSCA
                     TAASERGHQVELFEASPRIGGQFNLARCVPGKEEFGETIRYFNNKIEKIGVSRHLGHR
                     ATAAELLSLDFDVIVLATGTQPRKASIPGIDHPSVVSYADVITGAVSVGKRVAILGAG
                     GIGFDVAEVLTHTEPSTSQDIEAFLRVWGVDTTVRAPGGVVETTKAGQTSPRQVYLLQ
                     RKRGKPGVNLAKTTGWIRRATLERRGVEMIPGATYQSIDDAGLHIIVDGISRTIEVDH
                     IVPCVGQVPLRDLEEELVAVKKPVHLIGGAAKAAELDAKRAIEEGLRLAAAL"
     gene            complement(2406..3254)
                     /locus_tag="PK710_14470"
     CDS             complement(2406..3254)
                     /locus_tag="PK710_14470"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HOT10980.1"
                     /translation="MFSYVDPTVKRDLLREGKLIFLDEYGRECADHKHPNPLISMVGP
                     VPLPVSLCPGEPRFHWYAWVKHSDLHRIEQVVEQVRLEGPRMLFALLSESMAVNSVLL
                     FGDFEHARAPLVRVHSNCLTGDVFGSMRCDCGPQLQHALERIHNEGAGALVYMAGHEG
                     RGIGLWAKAITYLLQDAGQDTYQANEKLGLPADSRDFHDAALVVRYFRGANDDIRLLG
                     NNPMKREALEAVGLRVTQEPQVVGLTRFNIRYLTSKQQKGHIIPKDALDSIAPASAAD
                     DDVHGT"
     gene            complement(3312..5171)
                     /locus_tag="PK710_14475"
     CDS             complement(3312..5171)
                     /locus_tag="PK710_14475"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOT10981.1"
                     /translation="MPRSFVAKLGIACSSLAWILCVAPEAKAAPMHVSFVDVDSASYG
                     SYVGVYGKGFGDTKGQISFGGATSIDMPLWSDSLIIAKIPAGAQTGIVKVTAGSEQAE
                     SPESLKIHSGAIFVVSSAGSDSNSGDTEHPFQTLHKAMSVVQPGDTVLIQAGAYDEPD
                     LDTSIPLPAMYFRNNSGGTAEKPITWRGYGADVPVIRASHSLSSTSPVLLVEGEYLRF
                     ARIEINGTNNTSSGVSVWASNTWVVGLDVHSFGETGILVGETDATMVAANRIHNGGTR
                     ADLDHGILMLGQHATIRNNDISDLPNGYGIFLQYQSQEFAYVFENFIHDIAGGGIGLS
                     RVGGNNRIFNNIVWKAGLSQGCRCALQVAYGQAASEPSSTDRIYYNTFVGPGVAGLFV
                     ADREGSIEMHGNIFSEFKAGIRIDDTGPWPYLSTSHNLWHGQKGAPQFKLGGPWIDFA
                     QFQSQTGQEDGSLVADPKLVDPEAGDMHLSAGSPAIRAGGGPDRPASDFDGVARPSEA
                     VAPDMGALQFDGPGGSGGSAGSGGSGGDPDSGSAGSGAEAGSGGSSEGGSGNASGSGG
                     SGGGASAGKGAASQNDTSDDDGGGCGCTTPSSPTHSPLALLVALGALAWRRRR"
     gene            complement(5282..6700)
                     /locus_tag="PK710_14480"
     CDS             complement(5282..6700)
                     /locus_tag="PK710_14480"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOT10982.1"
                     /translation="MITVPVSSPAPHRLCRVLIVSKEASLRRDLARTLMSEHEMLSAE
                     DEATARSLLSSTSVDVLLFDAAGFDPDLPTLLLESHPALTLVLLTDLYPNPDTPWPQR
                     LFLTLPRSACPPESMAQVVHRAQQYKQLTERIAARERQPDRAAPVQIVGSSRAMDPVF
                     ELAHAAATCAIPVLLVGEKGTGKETLARAIHHQGHGASGPLVVVECTAMAPESLTREL
                     FGPLDSALRPPLAVAAHEGTLLLANIESLTLESQAQLVRLLQYGEIAADQAQPQHANV
                     RVMATTTQDLRPLVRAGTFRQDLLIPLKTLTIRIPPLRQRPDDIPLLAYHFVKRHADR
                     MGRAFDRISPDALRILAQHRWPDNVRELEAVMARAAAFSRGNVILPSDIARAIERAIS
                     PVDLEFDAKSQEFSFDPGVWDLPYSEAKEQARTSFDRQYVQQMMRRAGGNISEAARQS
                     GLDRSNFRRIMRKTADRRDDGS"
     gene            complement(6910..>7377)
                     /locus_tag="PK710_14485"
     CDS             complement(6910..>7377)
                     /locus_tag="PK710_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10983.1"
                     /translation="VAPFVNPAPPIRVLPSPAMPVPPRSAVLLLLGLTFASCRSSEAD
                     PSPPAPAMPASWAVKDDQTYSHNQREFLEIEGRLKGRVKALRITLYEVDHHLVRLHTI
                     VPMNSEEADKMYRILANKKHPASYLRKDEIIYEFSGPEQVQDQIERAHDLLLP"
BASE COUNT         1513 a         2061 c         2258 g         1545 t
ORIGIN      
        1 ttcttacgcc ccggtcctaa tgtggaacga agaaaggatc tgtagtacta gttcctaccc
       61 ccgtctccca tccccccagc atgtccaggc acgactcccc ctcgtctcca ccatcgcacg
      121 atacaccgaa atggctcttg cccttggtag cgaaccaagg attcgaaaag cggcttgctc
      181 aagactcgcc cgggttgttc cgcgccgtgg tacttcgttc gatatgttgt aactattgaa
      241 taatattata ttattcaaga attcgaccgg gcgttttccc gatggggcgc gtcgttctgg
      301 aacaatgcca gtgtcctgtc catactgctg ccgtgtccaa tccctatccc catctgctcg
      361 cgcccctcga tcttggtttt gtcaccctgc gcaaccgtgt gctcatgggg tcgatgcata
      421 cggggctcga ggactcgatt tggaattacg gcaagcttgc ggcgtatctt ggcgcgcgcg
      481 cgcgcggtgg tgtggctctc atcgtgacgg gcggctatgc acccaatcta tcgggaaggg
      541 tggcgccgtt ggcaggcaag ctcacccatc gtttcgagac catcaaacat cgcccgatga
      601 cacgcgccgt gcatgatgag ggcgggcgta tctgccttca gatcttgcac gcgggccgtt
      661 atgcctttca tcccctttcg gtagctcctt ccgccattcg ctctcccatc agccctttca
      721 agcctcgcgc tctttccgat cggcagattc gaaaaaccat ttccgacttc gttcgctgcg
      781 cccagctcgc cagagaggcg gggtacgacg gcgtggaaat catgggctcc gagggctacc
      841 tcatcaacca attcctcgcc gagcggacca accaacgcac ggacgcatgg ggaggaacgt
      901 tcgatcatcg aacgcggttt gccgtcgaga tcgtacgcca aacacgagag gcgttgggac
      961 cggacttcat cgtcatcttt cgtctttcca tgctcgattt ggttccgaga gcaagcgact
     1021 ggcaacaggt cgttacgctc gccaaacaga tcgaacgcgc gggggcaacg atgatcaata
     1081 ccggcatcgg atggcatgag tcccgcgttc ccaccatcgc tacggtagtg cctcgcgctg
     1141 ccttcacctg ggtgactcgc aagctaaaaa cggaagtggg gattcccctc atcacgagca
     1201 accgcatcaa tgcgcctgat gttgcggaag aggcgctggc acggggcgat gcggatatgg
     1261 tgtcgatggc gcgggcgttg ctcgccgacc ccgatttcgt tcgcaaagcc gagcagggaa
     1321 gggccgatga gatcaacacg tgcattgcct gcaaccaagc ttgtctcgac catgttttcg
     1381 ccaatcggcg cgcgtcgtgt ctggtcaatc cgcgcgcgtg tcgtgaaacc gagatccgtt
     1441 tgcatcccgt tcgaaggcgt aagcgcgtgg cggtgatagg ggccggtccg gcggggttgt
     1501 cttgcgccac ggccgcctcc gaaagaggcc atcaagtaga attgttcgaa gcctcccctc
     1561 ggataggcgg ccaattcaac ctcgcccgat gcgtgccggg caaagaagag ttcggcgaga
     1621 cgattcgtta tttcaataat aaaatcgaaa agataggtgt aagccggcat ctcggacatc
     1681 gggctacggc ggccgaattg ctctcgcttg acttcgatgt catcgtcctc gccacgggaa
     1741 cccaaccgcg aaaagcttcg attcccggta tcgatcaccc cagcgtggtt tcctacgccg
     1801 atgtgatcac cggagccgta tccgtgggaa aacgcgttgc catccttggc gcgggaggga
     1861 tcggtttcga tgtggcggag gtgctgaccc acacggagcc ttcgacgagt caggacatcg
     1921 aggctttcct tcgcgtttgg ggagtcgata ccacggttcg cgcgccggga ggcgtggttg
     1981 agacgacaaa agccgggcaa acatcgccca gacaggtgta tttgctacag cggaaacggg
     2041 ggaagccagg agtcaatctg gccaaaacca ccggttggat ccgtcgagcc accctcgagc
     2101 gacggggcgt cgagatgatt cccggggcga cgtatcaatc gatcgatgac gccggacttc
     2161 atatcatcgt ggatgggata tcccgcacga ttgaggttga ccatatcgtg ccgtgcgtag
     2221 gccaagttcc gttgcgggac ctggaggaag aactcgtggc cgtgaaaaag cccgtccatt
     2281 tgatcggggg ggccgcgaaa gccgcggaac tggatgccaa acgcgcgatc gaggaaggtc
     2341 ttcgtttggc tgctgctttg tgaaaacctc gcatcgatca caacgcgacg gctccccacg
     2401 gcagcttatg ttccatggac atcatcgtct gccgcgctgg cgggagcgat gctgtcaagg
     2461 gcatctttgg ggatgatatg tcccttttgc tgcttggaag taaggtagcg gatgttgaat
     2521 cgagtcagcc caacgacctg gggttcttgg gtgacgcgta agccaaccgc ttccaacgct
     2581 tcgcgcttca tcgggttatt tcccagcagg cgaatatcgt cgttggcgcc tcgaaagtaa
     2641 cggacgacca aggcggcgtc gtggaagtct cgagaatcgg cggggagccc cagtttttca
     2701 ttggcttggt aggtgtcttg tccagcgtct tgcagaaggt aggtgatggc tttggcccac
     2761 aggccgatgc ctcttccttc gtgtcctgcc atgtatacca acgctcctgc gccttcgttg
     2821 tggattcgtt cgagggcgtg ctgcaattgg gggccgcaat cgcagcgcat cgagccaaaa
     2881 acatcgcccg tcaggcagtt ggaatgcacg cggaccagag gggcccgcgc gtgctcgaag
     2941 tcaccgaata gcagcacgga attgaccgcc atcgattcgg acagcagggc gaaaagcata
     3001 cgaggccctt cgaggcgaac ctgttcgacg acctgttcga tgcggtgcaa gtccgaatgt
     3061 ttgacccaag cataccagtg aaagcgaggt tctcccggac ataagctgac gggaagaggc
     3121 acggggccca ccatgctgat gagtggattg ggatgcttgt ggtccgcgca ttcgcgtccg
     3181 tattcatcca aaaaaatcag tttcccctcg cgaagcaaat cgcgcttcac cgtcgggtct
     3241 acataagaaa acatggcggg accatggtca aaggccccgc gattgccaag cgttttcgtg
     3301 gaacgatgag gttagcgtcg acggcgccag gccagggccc cgagcgctac gagcaacgcc
     3361 aagggtgaat gcgtggggga ggagggggtt gtgcatccac agccgccgcc atcgtcatcg
     3421 gaggtgtcat tctgggatgc ggcacctttg ccagcgctgg ctcccccgcc cgagccaccg
     3481 gagccgctgg cgttgccgga gcctccctcg ctcgaaccac cggagccggc ttctgctccc
     3541 gagcccgcgg agccgctgtc cggatcgccc cccgagcctc cagaacccgc ggagcctccg
     3601 gaaccacccg gaccgtcgaa ttgtaaggct cccatatccg gggcaacagc ctctgaaggc
     3661 cttgcaactc catcgaaatc cgatgctggc ctgtccggtc cgcccccggc gcgaatcgcc
     3721 ggactgcccg ctgacaaatg catgtccccc gcctcgggat cgacgagctt ggggtccgct
     3781 accaacgatc catcctcttg accggtctgg gattggaatt gagcgaaatc gatccaaggt
     3841 ccgccgagct tgaattgggg cgcgcctttt tgtccgtgcc agaggttatg cgacgtggac
     3901 aaataaggcc acgggccggt gtcatcgatg cggatgcccg ctttgaattc cgaaaagatg
     3961 ttgccgtgca tctcgatgga gccttcccga tcagcgacga acaggcctgc caccccggga
     4021 ccgacgaagg tattgtaata aatgcggtcg gtggacgagg gttcactggc agcttgtccg
     4081 taagcgactt gcagtgcgca acggcatccc tggctcaacc cggctttcca tacgatattg
     4141 ttgaagattc tgttgttgcc gccgacgcgg gacagtccaa tgccaccgcc cgcgatgtcg
     4201 tgaatgaagt tttcgaaaac atacgcgaat tcttgcgatt ggtactgcaa aaaaatgccg
     4261 taaccattgg gtaggtcgct gatatcgttg tttcggatgg tcgcgtgttg cccaagcatc
     4321 aggatgccat ggtcgagatc ggcccgtgtt ccaccgttgt gaatacggtt ggcggcaacc
     4381 atcgtggcgt ccgtttcccc caccaagatt ccggtttctc caaaggagtg tacatcgaga
     4441 cccacgaccc atgtattcga agcccacacc gaaaccccgc tggaagtgtt gttcgtgcca
     4501 ttgatctcga tacgggcgaa acgcaaatac tcgccttcga cgagcagcac ggggctggtg
     4561 gatgataggg aatgagaggc tcggatgacg ggtacgtccg cgccatagcc tcgccaagtg
     4621 atcggttttt cggcggttcc tccactgttg ttgcgaaaat acatggctgg caacggaatc
     4681 gaggtatcca gatcgggttc gtcgtaggcc ccggcctgga tcaacaccgt gtcgcccggt
     4741 tgcacgacgg acatggcttt gtgaagtgtt tgaaaggggt gctccgtatc gcccgaattg
     4801 ctatcgcttc cggcggaaga caccacgaat atggctccgg aatgaatctt gagcgattcg
     4861 ggtgattcgg cctgttcgga tcccgctgtc actttgacga tgccggtttg ggcgccggcg
     4921 ggtattttcg caatgatgag gctgtcggac caaagcggca tgtcgatcga cgtagcacca
     4981 ccaaacgaga tctgaccttt ggtgtctccg aagccctttc cgtatacacc cacgtaagag
     5041 ccataactcg ctgaatccac atcgacgaag ctcacatgca tgggggctgc ctttgcctcg
     5101 ggcgccacac atagaatcca ggccagtgag ctacaagcaa tgcctagctt cgcaacgaat
     5161 gatcggggca tgttctcgac ttctttctcc aatcgtcgca actgtcaatg aatcataggc
     5221 tagcacgttc gcccccgcac tcaccagggc tagcgaatcg cttcgagaaa gcccgtagga
     5281 ctcacgatcc gtcgtctcgt cgatccgcgg tttttctcat gattctccgg aagttggaac
     5341 gatccaatcc gctctgacgc gcggcttccg aaatatttcc gcccgcccgt cgcatcattt
     5401 gctgcacata ttgtcggtca aacgacgtgc gagcttgttc tttggcttcc gaatagggaa
     5461 ggtcccatac accaggatcg aacgaaaact cctgcgattt cgcgtcgaat tcaaggtcga
     5521 cgggtgagat ggctcgctcg atggcgcgtg cgatatccga cggcaagatg acgtttcccc
     5581 gggaaaaagc cgcggctctt gccatcaccg cttccaattc gcgaacattg tccggccagc
     5641 gatgctgagc tagaattcgc agcgcgtcgg ggctaatccg gtcgaaagcg cgtcccatcc
     5701 ggtcggcatg gcgcttgacg aaatgatacg ccaacaaggg gatgtcgtcg ggtcgttggc
     5761 gcagcggggg aatccgaatc gtcagtgttt tcaaggggat gagtagatcc tgacgaaagg
     5821 tccccgcgcg aacgaggggt ctgaggtctt gggtggtggt ggccatcaca cgcacgttgg
     5881 cgtgttgggg ttgcgcctga tccgcggcga tttcaccgta ttgcagcagg cgaacgagct
     5941 gggcctggga ttcgagggta agcgattcga tatttgccaa caacagcgtg ccttcatggg
     6001 ccgctacggc gagcggtggg cgaagggcgg aatccaaagg accaaaaagc tcgcgtgtca
     6061 ggctttcggg agccatggca gtgcattcca cgacaacgag cgggccagaa gcgccgtgcc
     6121 cttggtggtg aatggcgcga gccaacgttt cctttcctgt ccccttttcc cccacgagca
     6181 acacggggat ggcgcaagtc gctgccgcgt gcgccaattc gaataccgga tccatcgcac
     6241 gggaagaacc tacgatttgc accggggcag ctcgatccgg ttggcgctcg cgcgccgcga
     6301 ttcgctccgt gagttgcttg tattgctgag cgcgatgcac gacctgcgcc atggattccg
     6361 gtgggcaagc gcttcgggga agggtaagaa acagtcgctg cggccaggga gtatccggat
     6421 tcggatacaa gtccgtgaga agcacgagcg tcagggcagg gtggctttcc aacaaaaggg
     6481 ttggcaggtc agggtcgaaa cccgccgcat cgaaaagcaa tacatccacc gatgtgctgg
     6541 aaagcaaaga cctggcagtc gcttcgtctt cggccgagag catctcatgc tcgctcatga
     6601 gggtgcgtgc gagatcgcgt cgcaacgacg cttctttcga aacgataagc acacggcaaa
     6661 gacgatgggg agcaggggaa gaaacaggca cggtaatcac ggtgatgcgt tgttatggcc
     6721 gaggattcaa ggggtgaaac ggtttcgtca agccaagggc atggggcggg tatcgctgcg
     6781 agctgccgaa agccgggtca ttggatggag gagtcggaat gtttcgcgaa agtcaggggc
     6841 agattggtga aaatatgtgt aaatacaaat agataacgta tgcggatacg gatggcctct
     6901 tctctgggat catggcagga gcaggtcatg agcccgttcg atctggtcct gaacttgctc
     6961 ggggcccgag aattcataga tgatttcgtc ttttcgaaga taggaggcgg ggtgcttttt
     7021 gttggcgagg atacggtaca ttttgtcggc ttcctcgctg ttcatgggga cgatggtgtg
     7081 gagtcggacg aggtggtggt cgacctcgta cagggtgatt cgcagggctt tgacgcgacc
     7141 ttttagtcgt ccttcgattt cgagaaactc gcgttggttg tgggaatagg tctggtcgtc
     7201 tttgacggcc cacgacgcgg gcatggcggg ggcagggggg gacgggtcgg cttcggacga
     7261 ccggcatgag gcgaaggtca ggccaaggag caagagcacg gcagagcgtg gaggaacagg
     7321 catggcgggg gatggtagca cgcggatggg cggtgctgga ttgacgaagg gagccac
//
LOCUS       DAOSOR010000273         7376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10097_length_7376_cov_4.407868, whole genome shotgun sequence.
ACCESSION   DAOSOR010000273 DAOSOR010000000
VERSION     DAOSOR010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7376
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10097_length_7376_cov_4.407868"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..3016
                     /locus_tag="PK710_14490"
     CDS             29..3016
                     /locus_tag="PK710_14490"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT10984.1"
                     /translation="MNVVPAALSGVHATVLTDREKVLQTAQKYVEKKRFDRAIAEYQK
                     IVREDPNDARTLLKIGDLQARTQDFDAAIATYERVARYYAAQGFLLKAIAVYKQIRES
                     IRKQPAELTEKYAHIIPQLAQLYQDLGLTSDALAAYDEYAAHLQRSGRNHEAIEIFQK
                     IVEINGNNPLTRLRLAEALLGQDRTDEALSQYSMASEILLGMGRSDDALKVIERMLFQ
                     KLDPALARRAAEIYLDRGRDDDGMQALSKLQICFQADNRDLNTLMLLSRAFEAIGQAP
                     KATEVRKEIARIARDKGEFAIAKEAAQALIKEAPEDEAVQALARSILAAERASAFPAP
                     RSEPPPSVQVSTVTSEDAVIEVSEEAVVFSDMVSVELEIESSDAAEPEGVLEVSAVHQ
                     EDFGLGDGFEELEPLTDTEVGVRPTMDEAPRSIRTVRVPEALEPVEEVGIEPFDMAAY
                     AQQVLANASAFRSHHLIDQAIETLRAGLDVQPDAFDLRFALKEMLIEAGDYSSACEEL
                     MIIAGMYLKAQDVQSGAQLLSEVLALDPLHIGARDLLVHLGYDISSWTDRSSRSAIDS
                     VPHSTSDFMQEVDSFSGGYERAEPLPSYDLEEINPSYAMSSVPAAEQADYSSLLTADD
                     PFAAMDGMQRMPFQTLLDDPFVEKEPLPSFPLSDEDRGPESMDDLHIGFAAPSSVRED
                     YFGAEFQQDASGFGQEDSHSPRVEATDISLSPLAGTRGFRGGDSLEDALEEADFFASR
                     GLFEDAMGVLEEQMGRFPNHPLLLERAREIAEAMAGEASDDHRQSQAPYLESAHDNAF
                     DIAASLEQMDVEPFSPQPQIPEGFGKPVDVEEVFAKFKEGVKAQVSESDSQTHYDLGM
                     AYKEMGLHSDAIAEFTMAARDAKRECVCWSMIGLIRTEIGDLDGAIEAFIRGLHAEHK
                     TVEQELALYFELGAIYQSRDNPQEALYYFDKVMRRDPGFRDVSQRVRTLQPSTQPARP
                     AASDMDDFDQAFADLFDAGKPSR"
     gene            3168..3551
                     /locus_tag="PK710_14495"
     CDS             3168..3551
                     /locus_tag="PK710_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT10985.1"
                     /translation="MRLCATHRIAVLLSTSVVACGGYVDRGRNLYEQGRYIESAEVLQ
                     HYEQEIGSEPPSRQAEYATYRGLSMLVLGNYEEARRWMLQAYAIGRTYPGSILPAWQR
                     DLDRGWRQLQPHLLSPQGQSAPPTR"
     gene            complement(3581..5641)
                     /locus_tag="PK710_14500"
     CDS             complement(3581..5641)
                     /locus_tag="PK710_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HOT10986.1"
                     /translation="MGESKMSMVDAIAKMQNYDLYRDLNWEGSFEDYLQIVRDKPQVT
                     RNAFQRVYDMIVSHGTEEYIDNKKRLVRYNFFRDEANHEADAIYGLDIPLMRLVHVFK
                     AAAQGYGPEKRVVLLHGPVGSSKSTIVRLIKKGLEQYTRIPEGALYTFRWVNLREMGL
                     STQEDSMNCPMHEEPLRLIPQSWREKTIDDLGLSNDRFRVRVQGDLDPACRFIYRHLL
                     QAYQGDWAKMISKHVRVYRLVMSEQDRIGIGTFQPKDEKNQDSTELTGDINYRKIAEY
                     GSDSDPRAFNFDGEFNIANRGIVEFVEVLKLDVAFLYDLLGASQEHKIKPKKFAQTDI
                     DEVIIGHTNEAEYTKLLNNEFMEALRDRTIKIDIPYITRLSEEIRIYEKDFSAQKVRG
                     IHVAPHTLEVAAMWAVLTRLEDPKKHSLSLIQKLKLYDGKVLPGYTQDNVKELRKESK
                     REGMEGISPRYVQDKISNVLVNDMGEGTINPFMVLNELEKGLRHHSLISSDEQRKRFA
                     EAIGIVKLEYEDIVKNEVQRAISADDDSIDKLASNYIDNVKAFTLREKVRNRYTGQDD
                     EPDERLMRSIEEKIDIPENRKEDFRREIMNFIGALAVDGKRFDWRTNERLRTALQRKL
                     FEDQKDSIKLKTLVASVVDKKTQEKIDTIKDKLIQYYGYNEVSATDVLNFVASIFARG
                     DAKE"
     gene            complement(5976..6311)
                     /locus_tag="PK710_14505"
     CDS             complement(5976..6311)
                     /locus_tag="PK710_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10987.1"
                     /translation="MASKFSDFLTEKKIDPRSVLAASKMIERLRPEDRAIRLKERIAR
                     RSEDGMPEEMKKNRVKPKSGKPVTRPAMQAALEGKAISGPLKTRLVRAVNHILQVKKL
                     NQVDIRTLF"
     gene            6414..7259
                     /locus_tag="PK710_14510"
     CDS             6414..7259
                     /locus_tag="PK710_14510"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOT10988.1"
                     /translation="MSTQSITGLLDEAVSAIRLVHAQRPTIGVVLGSGLGAFGDTLED
                     ASRIGYQDIPNMPMSRVVGHAGVLSLGRVENVPVACMQGRVHLYEGHEPERVVFGVRM
                     LARLGCEAVLITNAAGGVHPSLLAGDLMLIIDHINMTGRNPLVGPNIDEFGTRFPDMT
                     EAYDRGLCEVARKAARIEGISLHEGVYQANLGPTYETPAEIRMARTLGADAVGMSTVP
                     EVIALRHMQVRTAAISCITNLAAGISSHPLDHKEVEQVANRTRADFVRLIRRFVILSA
                     QEIAS"
BASE COUNT         1507 a         1927 c         2158 g         1784 t
ORIGIN      
        1 cgtagatttc catgaatggc cgcgcgctgt gaatgttgtt cctgcggccc tctccggagt
       61 ccacgctacc gtgctcaccg accgagaaaa ggtcctccag actgcccaga agtacgtcga
      121 gaagaagcgg tttgaccgag ctatcgccga gtaccaaaag attgtccggg aggatcccaa
      181 cgatgcgcga acgttgttga agatcggtga tcttcaagcg cggacacagg atttcgatgc
      241 ggcgatcgcc acctacgagc gggtagcgcg ctactatgcg gcgcaggggt ttttgctcaa
      301 ggccatcgcc gtatacaaac agattcggga gagcatacgc aagcagcccg ccgaactgac
      361 ggagaagtac gcgcacatca tcccgcagtt ggcgcagctt tatcaggacc ttgggctgac
      421 gagcgatgcg cttgcggcct acgacgagta tgcggctcac ttacagcgtt ccggtcgaaa
      481 tcatgaagcc atcgagatct ttcagaagat cgtcgaaatc aacggcaaca atccactcac
      541 acgattgcga ttggccgagg ctttgttggg gcaagacagg acggacgagg cgttgagtca
      601 gtactcgatg gcttcggaaa ttctgctcgg gatgggccga agtgacgacg cgttgaaggt
      661 gatcgagagg atgctttttc aaaagctcga tccggcgttg gcgcgtcgag cggcggaaat
      721 ctatctcgat cgtggtcgtg atgacgatgg catgcaggct ctttcgaagc tgcagatttg
      781 ctttcaagcg gacaatcgag atctcaacac gttgatgttg ctttcgagag cgtttgaagc
      841 gattgggcag gcaccgaaag ccacggaagt gcgcaaggag atcgcgcgaa tcgcaagaga
      901 caagggcgag tttgcgattg cgaaagaagc ggcgcaagcc ttgatcaaag aggcgcccga
      961 agacgaagcg gtgcaagccc tggcgcgctc gattctcgcg gcagagcgcg catcggcatt
     1021 tcctgcgcct cgttccgagc cgcctccgtc ggtgcaggtt tcgacggtaa cgtccgagga
     1081 cgccgtgatc gaagtgtcgg aagaggccgt tgttttctcc gatatggtga gtgtcgaact
     1141 ggaaatcgaa tccagcgatg cggccgaacc cgaaggggtc ctcgaggtta gtgcggttca
     1201 ccaagaggac tttgggcttg gtgatgggtt tgaggagttg gagcctttga cggataccga
     1261 ggttggcgtt cggcccacga tggacgaagc tcctcggtcc atccgaacgg ttcgtgttcc
     1321 cgaagctctc gagcctgtcg aggaagtggg tatcgaacct ttcgacatgg cggcttatgc
     1381 gcagcaagtc cttgcgaacg cgagcgcatt tcgaagtcac cacttgatcg accaggccat
     1441 tgaaacctta cgggctggtt tggatgtgca acccgacgct ttcgatctgc ggtttgcgtt
     1501 gaaagaaatg ctcatcgagg ccggggatta ttcgagcgct tgcgaagaac tcatgatcat
     1561 cgcgggcatg tatctcaagg cgcaagacgt gcaatcgggt gcacaattgt tgtccgaagt
     1621 tcttgccctc gatcccctcc atatcggcgc acgagacctt ttggttcatc ttgggtatga
     1681 catttcttcg tggacggata ggtcttctcg ttcggcgatc gacagtgttc cgcattccac
     1741 ctctgatttc atgcaggagg ttgattcgtt ttccggaggt tacgaacgag ccgaaccgtt
     1801 gccttcctat gacttggaag aaatcaatcc ttcttatgcc atgtcatcgg ttcctgccgc
     1861 ggagcaggct gattattcgt cgctgttgac ggccgatgat ccttttgcag cgatggatgg
     1921 aatgcagcga atgccattcc aaacgctgct cgatgatcct tttgtggaaa aggaaccgct
     1981 gccgagtttc ccgttgtcgg atgaggatcg ggggccagaa tcgatggatg acttgcacat
     2041 cggttttgct gctccttcct cggtgagaga agattatttc ggtgctgaat ttcagcaaga
     2101 cgcctccggt ttcggacagg aggattcgca ttcacctcgc gtcgaagcga ccgatatatc
     2161 gctttcgcct cttgcgggaa ccagagggtt tcgcggaggg gattctctcg aagatgccct
     2221 tgaagaagcg gatttttttg cgtcacgggg actattcgag gatgcgatgg gggtgctcga
     2281 agagcaaatg gggcgctttc ccaatcatcc attgctgttg gagcgtgcaa gagagatcgc
     2341 tgaagccatg gccggggaag ccagcgacga tcataggcag tctcaagctc catatttgga
     2401 gtcagcgcat gacaatgctt tcgacattgc cgcttcgctc gaacagatgg atgtggagcc
     2461 gttctcaccg caaccgcaga ttcctgaagg ttttggcaag ccagtggatg tggaggaggt
     2521 tttcgccaag ttcaaggaag gtgtgaaagc gcaggtttcg gaaagtgata gccaaaccca
     2581 ctacgacttg gggatggcct acaaagagat ggggcttcat tccgatgcca tcgccgaatt
     2641 cacgatggcc gcgagagatg caaaacgcga gtgtgtatgt tggtccatga tcgggttgat
     2701 tcgcaccgaa atcggggact tggatggcgc gatcgaagcc ttcatccgcg gcttgcacgc
     2761 ggagcacaag accgtggaac aagaactcgc gctgtacttc gaacttggcg ccatctatca
     2821 gtctcgggat aacccacaag aagcgctgta ctacttcgac aaagtgatga gacgggatcc
     2881 ggggttccgg gacgtttccc aacgtgttcg aacgttgcag ccatcgacgc aaccggctcg
     2941 acctgccgct tcggatatgg acgacttcga tcaggctttt gcggacttat tcgacgcagg
     3001 caaaccttct cgctgaacct gcgtgagtgg cgagaagcga tggtagtggg cgcattgccc
     3061 tggagccgat gctcgatacg atcgtgatgt gctttacacg ggtaccgtag cgtgcctgtc
     3121 gggccatgat acgctacggt gaagaccgga tgagccatgg gtgttcgatg agattgtgtg
     3181 caacacaccg gattgccgtt ctgttatcga cgagcgttgt ggcttgtggc gggtatgtcg
     3241 acagggggcg aaatctttac gaacaagggc gctatatcga atcggcggaa gtgctgcagc
     3301 attacgagca ggagattggt tccgagccgc cgtctcgaca agccgagtac gcaacctatc
     3361 gaggactatc gatgctcgtg ctgggaaact atgaggaagc gcgtcggtgg atgttgcaag
     3421 cgtacgcgat tgggcgaacg tatcccgggt cgatactacc ggcttggcag agggatctcg
     3481 atcgagggtg gcggcagttg caacctcatc ttttgtcgcc gcagggccaa agcgcgccac
     3541 ccacgcgcta aaccgtatgc ggaaaacgtt tagccaacgg ttattctttg gcgtcgccgc
     3601 gggcaaaaat cgaagccacg aagttcagga cgtccgtggc gctcacctcg ttgtagccgt
     3661 agtactggat gagtttgtct ttgatggtat cgattttttc ttgggttttc ttgtcgacca
     3721 ccgaagccac gagggtcttg agtttgatgg agtctttctg atcttcgaat agctttcgtt
     3781 gaagagcggt ccgaagtcgt tcattggttc gccaatcaaa gcgctttccg tcgaccgcaa
     3841 gggctccgat gaagttcatg atttctcgac ggaaatcctc ttttcggttt tcggggatgt
     3901 cgatcttttc ttcaatggat cgcatgagtc gctcatcggg ttcatcatct tgcccggtat
     3961 agcgatttcg caccttttct cggagtgtga aggctttgac gttgtcgatg tagttggaag
     4021 cgagtttgtc gatcgagtca tcatcggcgc tgatggcgcg ctgcacctcg tttttgacga
     4081 tgtcctcgta ttcgagtttg acgataccga tggcttccgc gaagcgcttt cgctgttcat
     4141 cgctgctgat gagcgaatgg tgacgcaaac ctttttcgag ttcgttgagg accatgaagg
     4201 gattgatggt gccttcgccc atgtcgttga cgaggacatt ggagattttg tcttggacgt
     4261 agcgggggct aattccctcc atgccctcgc gtttggattc tttccgaagc tctttgacgt
     4321 tgtcttgtgt atatccgggc aggactttgc catcgtagag cttgagtttt tgaatgagtg
     4381 acagggaatg ctttttgggg tcctcgagcc gggtgagtac ggcccacatg gctgccacct
     4441 cgagggtatg gggagcgaca tggatgccgc gcactttttg tgctgagaag tccttttcgt
     4501 agatgcggat ctcctcggat aggcgtgtga tgtagggaat atcgattttt atcgttcggt
     4561 ccctcagcgc ttccatgaac tcgttgttga gaagttttgt gtattccgct tcattggtat
     4621 gcccaatgat gacttcgtcg atgtctgttt gcgcgaattt tttcggcttt attttgtgtt
     4681 cttgagaagc gccgagcaga tcgtacaaga aggcgacatc gagtttgaga acctcgacga
     4741 attcgacgat tcctcgattg gcaatgttga attcaccatc gaagttgaaa gcgcggggat
     4801 cgctgtccga gccatattcc gcgatctttc tgtagttgat gtcgcccgtc agttcggtgg
     4861 agtcttggtt tttttcgtct ttgggttgga aggtcccgat gccgatgcga tcctgttcgc
     4921 tcatgaccag acggtagact cgcacgtgtt tgctgatcat tttcgcccag tcgccttggt
     4981 aggcttgcaa caaatggcgg tatatgaagc ggcaagcggg atcgagatcg ccttgaacac
     5041 gaacccgaaa ccgatcgttg gataaaccga gatcgtcgat agttttttcc cgccaggatt
     5101 gcggaatgag acgcaggggc tcttcgtgca tggggcaatt catgctgtct tcttgtgtgg
     5161 acagccccat ttcccgcagg ttgacccatc gaaatgtgta taaggcacct tccgggattc
     5221 gcgtatattg ttccaagcct tttttgatca ggcgaacaat ggtggacttg gacgatccga
     5281 caggcccgtg aagtaatact acgcgttttt ccggaccata tccttgggcc gcggctttga
     5341 acacgtgaac caggcgcatc agtgggatgt ccagcccgta gatggcgtcg gcctcatgat
     5401 tcgcttcgtc gcggaaaaag ttatatcgaa cgagccgttt cttgttgtcg atgtactcct
     5461 cggtcccatg gctgacgatc atgtcgtaca cacgctggaa cgcgttgcgc gtgacctgcg
     5521 gcttgtcgcg cacgatttgg aggtaatcct cgaaggaacc ctcccaattc agatcccgat
     5581 acagatcgta gttctgcatt ttcgcgatcg catcgaccat gctcatctta gattcaccca
     5641 tcgttaacct atcctcattg atcagggttg gtctgctcga cgctggatcg cggtccagtc
     5701 gccaaagctc aggtgctcag tcgcgagagg gtgtaagcat atcaggttag cgacgccacg
     5761 aggggttccg tcgccaaatc ctcccatggc tgcccccaaa aggcgctcga gaagggatga
     5821 ggggcgtatg atgcggtcct tggcgcgagg tggcagagaa tccataaggg ttgcccgagg
     5881 ggggatgaca aaacacaccc caaggcatcc caagcccgga accatgacac cctagctcgg
     5941 cggaaccatc caccgcgcgt gtgcgtgaac aggttttaga aaagcgtgcg aatatccact
     6001 tgattcagct ttttgacctg aaggatatga ttgacggctc gcacaagcct cgtcttgagc
     6061 ggaccggaaa tggctttgcc ttccagcgcc gcctgcatgg cgggtcgtgt aaccggcttt
     6121 cccgacttgg gcttgacccg attctttttc atctcctcgg gcatcccgtc ttcgctacga
     6181 cgggcgattc gctctttgag ccggatagcg cgatcctcgg gacgtagtcg ctcgatcatc
     6241 ttggacgcgg ccagcacgct acgggggtcg atcttctttt ctgtgagaaa gtcgctaaac
     6301 ttcgaagcca tgcggtgctg tctagccaaa acgctgcccc aacgccagcc cttcgtgccc
     6361 aaatccaaca tcctggctcc cctttgccgt catccgttgt agcctcctgc cccatgagta
     6421 cccaatccat caccggcctt ctcgatgagg ccgtctccgc tatccgcctt gttcacgccc
     6481 aacggccgac catcggcgtg gttctcggct ccggtctggg cgcgttcggc gataccctcg
     6541 aggatgcctc ccgcatcgga taccaggata tccccaatat gccgatgtct cgggtcgttg
     6601 gacacgcggg cgtgctgtcc ttgggacgcg tcgaaaacgt gcccgtcgct tgcatgcaag
     6661 gacgtgtcca cttgtatgaa ggccacgaac ccgaacgggt cgtgtttggc gttcgtatgc
     6721 tcgcccggct cggttgtgaa gccgtgctga tcaccaatgc ggcgggcggc gtacacccat
     6781 cattgcttgc cggtgacttg atgctcatca tcgaccacat caacatgaca ggccgaaatc
     6841 ctcttgtcgg cccgaatatc gatgagttcg gcacacgctt tcccgacatg accgaagcct
     6901 acgaccgtgg tctttgcgaa gtggctcgca aggccgcgcg aatcgaaggc atctcattgc
     6961 atgagggcgt gtaccaggcc aaccttggtc caacatacga aacgcccgcg gagattcgaa
     7021 tggcacgaac actcggcgcc gatgcggtag ggatgagcac ggtcccggaa gtcatcgctc
     7081 ttcgacacat gcaggtgcgc accgcggcca tctcttgtat caccaatctg gccgcgggta
     7141 tctcttcgca tcctctcgat cacaaggaag tcgagcaggt tgccaaccgt actcgcgcgg
     7201 acttcgttcg cctcatccga cgcttcgtta tcctatccgc acaggagatc gcatcgtgaa
     7261 tcgctctgcc atcgactggg atgcattggt cgatgccgct acggacgctc gatcgaatgc
     7321 gtatgttccg tattcgacgt ttcccgtggg agcggcgatc cttacgggtt cgggcc
//
LOCUS       DAOSOR010000274         7374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10101_length_7374_cov_4.638065, whole genome shotgun sequence.
ACCESSION   DAOSOR010000274 DAOSOR010000000
VERSION     DAOSOR010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10101_length_7374_cov_4.638065"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PK710_14515"
     CDS             <1..347
                     /locus_tag="PK710_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10989.1"
                     /translation="RLLGIQQGVEQGVQQGLEQGVKQGVKQGVKQGLEQGVKQGVKQG
                     VKQGVKKGLDKGLAPLVRLYERRLGRALSSRERTALQERLLKAGPERVGDVVLDLSAQ
                     QLADWLADSNAL"
     gene            585..1613
                     /locus_tag="PK710_14520"
     CDS             585..1613
                     /locus_tag="PK710_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOT10990.1"
                     /translation="MNAQSPHLPAVPAVAEAAELLHATQEAVGRLRQAVARAVVGQEQ
                     VVDLMLVTLFARGHALLVGVPGLAKTLLVSSIARALDLRFGRIQFTPDLLPADITGTD
                     VLHEELDDNGRAVRRLRFLPGPVFANLVLADEVNRTPPKTQAALLQAMQERHVTVGTV
                     THALPDPFQVFATRNPIEQEGTYALPEAQLDRFLLEIHVDYPSESEERQIAHKTTSGR
                     PVDIQPVLQGDEVRQIGSLVPRIPVTEEAVQLAVRWSRATRPGPDSIEMVRDYVRFGA
                     GPRGSQALVLCAKARAATRGEAVADVHDVRELLIPALRHRLVLSYRAEADGVQSADVL
                     HAIQRAVK"
     gene            complement(1796..1868)
                     /locus_tag="PK710_14525"
     tRNA            complement(1796..1868)
                     /locus_tag="PK710_14525"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1833..1835),aa:Gly,seq:ccc)
     gene            complement(1906..2250)
                     /locus_tag="PK710_14530"
     CDS             complement(1906..2250)
                     /locus_tag="PK710_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10991.1"
                     /translation="MKRSLAHAIASQPVDPVHRALVRARRARKKGQARHEVHALREAC
                     AHEEWDATLWTMLGAACMRQQRWDEAAAALRHALWLRERTDEPKRAMVTRKLLGLAQR
                     GAGVSTTLPFRR"
     gene            2334..3554
                     /locus_tag="PK710_14535"
     CDS             2334..3554
                     /locus_tag="PK710_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10992.1"
                     /translation="MDACEHGARTKNRFSFGFFAFFAVVAHGFGCGSPGEGVARPPPP
                     GWDGGQQDADGHDAQCSPKTCSMLGAQCGHVPDGCGGALACGQCPPNMVCGGSGPNQC
                     GTASCVPRTCVQLGACCGTVSDGCSQILDCGQCTPPEICGGTGVPNSCAPPCEEASKS
                     CAGTCVALDDPAFGCEMPLDPSTPCAPCPSAPNATPACQHGSCALACSPLWGDCDANP
                     ANGCETDLNSVDHCGDCKTTCSTTAGQPICTNGTCDVLIPTWSFTHWRVDQPSSTCAE
                     WGKVDEIQFLVAGTWAKNHATGYASGTVADKKVLSIDHSGDSSTYGVAWHAFDGTNAP
                     WQSFSNNSYFWQGSEFIRLQVAQPIEAQSVQVGTFWGSTRCVRLSGSHDGIQWTILGS
                     ACNTDCNSAVTFKP"
     gene            complement(3653..5224)
                     /locus_tag="PK710_14540"
     CDS             complement(3653..5224)
                     /locus_tag="PK710_14540"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOT10993.1"
                     /translation="MKTATAMVAVLMTVGCGGDRVEPIAPTPTRASTPQGNETAVAPD
                     IVVEKEELPPSEQTSVVVEPEPPLPRLGSVSHFTWIYKNTSRKQQPIGYVRPGTTVVL
                     DGQEPQGGSDCQSGRWYAVRPKGFVCEDGTVTLDVDSPLFVALTEVVRPREHVLPYRY
                     GYSMGAPMYGRFPSEKEREREAREYGRVDTLSRTEKIKSSYGELAMSDILPATDPVPV
                     FATPELPRLRGYTAALVRKTIPSGSLLSFGSAVESEGRTYLLSPDLTWVPADRVRPFR
                     ETTFHGIELGAQVRLPIGWTRSVPRAKMRKLQDGTMEPTGESWPPRTVLSLTGTVHTS
                     QGKRYHETQEGHWIRDTDVSVVEEKTTLPSTIQPGQKWIEFSSSRGTLTLYEGKTAIY
                     STLASPGRGGGAPSSKMSIEDLVQGSYTPLGTYRITFKTFSTTMTPEAVPNPKKHWIQ
                     DVPYTQYFLRPFAIHTAYWHEDFGMPKSGGCINLSPQDARYVFAWTEPVLPQDWSGVS
                     SSPEMGLGTMLVLRR"
     gene            complement(5369..6964)
                     /locus_tag="PK710_14545"
     CDS             complement(5369..6964)
                     /locus_tag="PK710_14545"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOT10994.1"
                     /translation="MDACALWERYKTYLVDHPSLGLRLDISRMKFGPHFFEEMAAPVE
                     RAFDAMAQLEKGAVANPDEDRQVGHYWLRTPSLAPTPEIATQIEHTVARIETFAQAVH
                     SGKIRPPGADRFTDILHVGIGGSALGPQLAQGALGKSTDAMRIHFLDNTDPDGIERVL
                     STLGAEGLSRTLVLVVSKSGGTKETRNGMLEVQAAFRKLGLAFGPQAVAITAEGSALS
                     KVATQENWLEQFPMWSWVGGRTSQTSAVGLLPAALQGFDIKAFLAGARDCDEITRLRD
                     ARRNPAMVLALMWLYSRSAGVAKDMVVLPYKDRLELFSRYLQQLIMESLGKSRDLSGN
                     VIQQGISVYGNKGSTDQHAYVQQLREGIHNFFVTFIDVVHDAQAAPTFVEDGQITSGD
                     YLLGFMLGTRRALYDNDRESMTLTIDRMNPRALGVLIALFERAVGYYATLVGINAYHQ
                     PGVEAGKVAANEVISLQKQVMQSMRSSKQAATVEAIATSIGRPDQAETIFKILEHLSA
                     NPGRGIKKHPAETPFNARFEAVD"
BASE COUNT         1538 a         2283 c         1961 g         1592 t
ORIGIN      
        1 gccggctgct ggggattcag caaggggtcg agcagggcgt ccagcagggt ctggagcaag
       61 gcgtcaagca gggcgtcaaa cagggcgtca aacagggcct ggaacaaggc gtcaagcaag
      121 gcgtcaagca aggcgtcaag cagggcgtaa aaaaaggcct ggacaaaggg ctcgctccgc
      181 tcgttcgtct ttatgaacga cgtcttgggc gcgcgttgtc gagccgcgaa cgaacggcat
      241 tgcaggagcg tttgctcaaa gcagggccag aacgcgtggg cgatgtcgtg ctggatttgt
      301 ccgcccagca gcttgcggac tggcttgcgg attcaaacgc cctctgatag ctcgtcgcat
      361 tttggctatg atacccgagc cttgagccac cgacttccaa taccttttct ctggaggaat
      421 ccttcgatgg atgatcgtcg aaaaacctca ttcccccatt ttttgagggg atgaagacac
      481 gaacgacgag cggtctcctc gagacggaag cattctctgt gaccaaggct tgcgcggtga
      541 gacgcaaagg tgcatgaaag ctcggcccat tatcgaggat gcccgtgaat gcccagtcgc
      601 cccatcttcc cgctgttccg gctgtggcgg aagcagcgga attgctgcac gcaacccaag
      661 aagcggtagg ccgccttcgg caggcggttg ctcgcgctgt cgtcggacag gaacaagtcg
      721 tggacttgat gctcgtgacg ctttttgctc gaggacatgc tttgctcgtt ggcgtgccgg
      781 ggcttgccaa aacccttctg gtatcgtcga ttgcacgtgc tctggacctt cgattcggtc
      841 ggatccaatt cacaccggat ttgcttcccg ccgacatcac cggcactgac gtcctgcacg
      901 aagaattgga tgacaacggc cgtgccgttc ggcgccttcg ttttctaccg gggccggtct
      961 ttgccaatct ggttcttgcc gacgaggtga atcggacacc tcccaaaacc caggcggctc
     1021 tgttgcaagc catgcaagag cgtcatgtga ccgtcggaac ggtaacccac gcgttgcccg
     1081 acccgtttca ggttttcgcg acccgcaacc ctatcgaaca ggaagggacg tatgcgttgc
     1141 cagaagccca actcgaccgg tttttgttgg aaatccacgt ggattatccc agcgaatcgg
     1201 aagagcggca gatcgcgcat aaaaccacat ccggtaggcc cgtcgacatt cagcccgtct
     1261 tacagggcga cgaggtcagg caaatcggaa gtctggtgcc tcgtatccct gtgacagaag
     1321 aagcagtgca acttgcggtt cgatggtcga gagccacacg tcctggaccg gatagcatcg
     1381 agatggtgcg cgattatgtt cgcttcggcg caggtccgcg cggaagccaa gctctggttt
     1441 tatgcgcaaa ggcccgggct gcaacacgag gagaggcggt agccgacgtc catgatgtgc
     1501 gcgaattgct gattccagcc ctgcgccatc gcttggtgct cagttatcgc gcggaagccg
     1561 atggcgtaca aagtgcggat gtgcttcatg cgattcagcg cgcggtgaaa taattgaccg
     1621 tagtcggcca tgcgccaacc cccaccgccg ccgtatcgtc gcttgccgtc tctttctaat
     1681 cggatgccct tcgcgaattc tctccgcgtt cgaaacgact cgttcccctc tgcgccggaa
     1741 cgcccgggac aaacgtaaat ggccggaatc aataaagatt ccggccaaaa aagggagcgg
     1801 gacacgggac tcgaacccgc gacccccagc ttgggaagct agtgctctac caactgagct
     1861 agtcccgcga agggatgcat gaagtactct tgccctgacc gcttgtcaac gacggaatgg
     1921 tagggtcgtc gatacgccag ccccccgttg cgctagcccc aaaagcttgc gggtaaccat
     1981 cgcccgcttg ggctcgtcgg tgcgctctcg cagccacaag gcatgccgaa gcgccgctgc
     2041 cgcctcgtcc caacgttgtt gacgcataca agcggctcct agcatcgtcc acaacgtagc
     2101 atcccattct tcatgcgcac aggcctcccg caacgcatga acctcatgcc gtgcctgccc
     2161 cttttttctc gctcgtcgcg cacgaacgag cgccctatgc accgggtcga caggttgact
     2221 tgcgatggca tgggccaagg atcgcttcat gctcctacag tcgagtgatc gccgagaatg
     2281 gccaagtttg cgtctcgcct tcaaacgtgt atcctccgct tttcaggtgc ttcatggatg
     2341 cttgcgagca tggagcgcgg acgaaaaaca gatttagctt tggctttttt gcgtttttcg
     2401 ctgtcgttgc ccatggcttt ggctgcggaa gcccaggaga aggagtcgcc agaccgcctc
     2461 ctcctggctg ggatggggga cagcaggatg ccgatggcca tgatgcgcag tgctcgccca
     2521 aaacttgttc catgctcggt gcgcaatgcg gccacgtgcc agacggttgt ggtggcgcgt
     2581 tagcgtgcgg acaatgccct cccaatatgg tgtgcggagg ttccggtccc aatcaatgtg
     2641 gcaccgcttc ctgcgttcct cgaacctgcg tccaactcgg agcatgctgc ggcaccgtat
     2701 cggatggatg cagtcaaatc ctcgattgcg gacaatgcac accgccagaa atatgcggcg
     2761 gcaccggtgt gcccaactca tgtgctccgc cctgcgaaga agccagcaaa tcctgtgcgg
     2821 gaacctgcgt cgccctcgat gaccccgcat tcggctgcga aatgcccctc gacccatcca
     2881 ccccctgcgc cccttgccct tccgccccca atgccacacc ggcctgtcaa cacggctcct
     2941 gcgccctcgc ctgttcgcca ctgtggggtg actgcgatgc aaaccccgcc aacggctgcg
     3001 aaaccgacct caactccgtc gaccattgcg gcgattgtaa aaccacttgc tcgaccaccg
     3061 cgggacaacc gatttgcacc aacggaactt gcgatgtgct gattcccact tggtctttca
     3121 cccattggcg ggtcgaccag ccctcctcga cttgcgccga atggggaaaa gttgatgaaa
     3181 ttcaattcct tgtcgcggga acgtgggcca agaatcatgc gacgggctat gcctcaggca
     3241 ccgtagcgga taaaaaagta ctcagcatcg accattctgg tgactcctcc acctacggag
     3301 ttgcctggca cgccttcgat ggtaccaacg ctccctggca aagtttcagc aataactcgt
     3361 atttctggca aggatccgaa ttcattcgct tgcaagtcgc tcagcccatc gaagcccaat
     3421 ccgtccaagt agggactttc tgggggtcga cacgatgcgt ccgcctctcg ggctcccatg
     3481 atggcatcca gtggacgatt ctaggttcgg cctgcaatac agactgcaac tccgccgtga
     3541 ccttcaaacc ctgagccaaa ggattgccaa accgcccacc ttgcatcgat gcgtggactt
     3601 gtgtctaact attgaatatg attgaataat ttctgtttgg ggcgggcgct acctaccgac
     3661 gaagcaccaa catcgttccc aaccccatct ccggactgct cgatacccct gaccaatcct
     3721 gcggaagcac gggctccgtc cacgcaaata cataccgcgc atcctgtggc gatagattga
     3781 tgcatccacc acttttcggc atcccaaagt cctcatgcca ataggcggtg tggatcgcaa
     3841 acggacgaag aaaatattgc gtatacggaa cgtcttggat ccaatgcttc ttcggattcg
     3901 gtaccgcttc cggtgtcatc gtcgtcgaga acgtcttgaa tgtgatgcga tacgtcccca
     3961 agggcgtata acttccttgt accagatcct cgatcgacat cttgctcgag ggcgctcctc
     4021 ctccccgtcc cggtgaagcc aacgtagagt aaatagctgt ttttccttca taaagcgtca
     4081 gcgtcccccg gctcgacgaa aactcgatcc acttctgccc cggttgaatc gtcgacggca
     4141 atgtcgtttt ctcttccact accgacacgt cggtatcccg gatccaatgc ccctcttgcg
     4201 tctcatgata ccgcttgcct tggctggtat gtaccgttcc tgtaagtgac aacaccgttc
     4261 gcggaggcca tgactcgccc gtcggctcca tcgtgccatc ctgcaatttg cgcatttttg
     4321 cccgtggaac cgaacgcgtc cacccgatgg gaagacgaac ctgagcccca agttcgattc
     4381 catgaaatgt ggtttcccga aatggacgaa ccctatccgc cggaacccat gtcagatccg
     4441 gactcaataa ataggtacgg ccctccgatt ccaccgccga gccaaacgac aacaaggatc
     4501 ccgagggaat cgtcttgcgc accaaagccg ccgtataccc tctcaatcga ggcaattcgg
     4561 gcgttgcgaa gaccggaacg ggatccgtgg ccggcaagat atccgacatt gccaattccc
     4621 cataactcga cttgattttt tccgtacggg acaaggtatc gacccgtcca tactcccgcg
     4681 cttctcgctc ccgttctttt tccgagggga atcgtccgta catcggcgcc cccatcgaat
     4741 acccatatcg ataaggtaaa acgtgctctc gcggacgaac gacctccgtt aacgcaacaa
     4801 ataacgggga atccacatcg agcgtgaccg ttccgtcctc acatacgaac cccttgggcc
     4861 gtacggcata ccatcgtccc gattgacaat cactaccgcc ttgcggctcc tgcccatcaa
     4921 gaactaccgt cgttcccggg cgcacgtatc cgatgggctg ctgcttacga ctcgtatttt
     4981 tgtagatcca cgtgaaatga gacacggagc cgaggcgtgg caacggcggt tccggttcga
     5041 ccaccaccga agtctgctcg gatggtggca gctcttcttt ttccacgacg atatccggcg
     5101 ccacagccgt ctcgttgcct tgcggtgtgg atgctcgtgt gggtgtcggg gcgatgggct
     5161 ctacccgatc tcccccacat cctactgtca tcagcacggc caccatggcc gtcgctgtct
     5221 tcacgccatt cgttgaacac aaggccggcc tgaaacaagc ggccaagcgc atcttgggtt
     5281 gcccatcatc gtggacgaca ccacgcatca tcgacgtcat cggtttcgtt tttccactgc
     5341 acacgagaga tttcaataga tatgccgctc aatccaccgc ttcgaaacgc gcattaaaag
     5401 gtgtctctgc cgggtgtttt ttgatccctc gaccgggatt ggcgctcaaa tgctcgagga
     5461 tcttgaatat cgtctccgct tgatcgggtc gtccaatgct tgtggcaatc gcttccaccg
     5521 tagcggcttg tttcgacgat cgcatgctct gcatgacttg tttttgcagt gaaatcacct
     5581 cgttcgccgc gactttcccc gcctccacgc ccggctgatg ataggcattg atccccacca
     5641 gcgtggcgta atatcccacc gctcgctcga acaaggcgat gagcactccc agcgcccgcg
     5701 gattcatccg atcgatcgtc aaggtcatcg attcccgatc gttgtcgtac agtgctcgac
     5761 gagtcccgag catgaatccc agcagataat cccctgacgt gatctgccca tcttccacga
     5821 acgtgggagc cgcttgcgcg tcgtgaacca catcgatgaa cgtgacgaaa aagttgtgaa
     5881 tcccctcacg gagttgctgc acataggcat gttgatcggt cgatcccttg ttgccataga
     5941 ccgaaatacc ttgctgaatc acgttgccgg aaagatcgcg gcttttgccg agcgactcca
     6001 tgatgagctg ctgaagatac cgggagaaaa gctccaatcg gtctttgtag ggtaaaacaa
     6061 ccatatcttt ggccaccccg gcggaccggg aatacagcca catcaaggcg aggaccatcg
     6121 cgggatttcg acgagcatcg cggagcctgg tgatctcgtc acagtcgcgg gctcctgcta
     6181 aaaacgcttt gatatcaaag ccttgcaaag cagcgggcag cagtcccacc gccgaggttt
     6241 gcgaggtgcg acctcccacc cagctccaca tcgggaattg ttcgagccaa ttttcttggg
     6301 tcgcgacttt cgacagagcg cttccttccg cggtaatcgc aaccgcctgg ggaccaaatg
     6361 caaggcccag ttttcggaaa gccgcttgca cctcgagcat tccgttgcgc gtttccttgg
     6421 tccctccgga cttggaaaca acgagcacca aggtgcggct caatccttcg gcaccgaggg
     6481 tcgaaagcac gcgctcgata ccatccggat ccgtattgtc gaggaaatgg atccgcatcg
     6541 catctgtgct ttttcccaag gctccctgcg cgagttgcgg gcccaacgcc gaacctccga
     6601 tccctacatg caggatatcg gtgaatcgat cggctcccgg gggcctgatt ttccccgaat
     6661 gcaccgcctg ggcgaacgtt tcgatcctgg ctaccgtgtg ttcgatctgt gtcgctatct
     6721 ccggtgtagg agccagcgat ggcgtccgaa gccaataatg acccacttgc cgatcctcat
     6781 cgggattcgc taccgctcct ttttcgagtt gcgccatcgc atcgaaagcc cgttccaccg
     6841 gcgccgccat ctcctcgaaa aaatgtgggc caaacttcat tctgctgatg tccagccgaa
     6901 gccccaaact cggatgatcg acgagatatg tcttgtaacg ctcccacaat gcgcacgcgt
     6961 ccatgctgtc ctccaatcgg cagagcgaat tcgtcgatcg agaaaggcaa accgtcaagg
     7021 cccttcgacc gattaatagt tgcccttccg ggggcccgcc gcaatcttcc cccaacgcct
     7081 cctccatcct gcatgaatcg aagcgaaagc cttcttcacc atcatcgcgt atcgtgaacg
     7141 tggtcgaaga aaaccatgcg cgttgccttc ctatctggac ttccccaccg tagagccgcc
     7201 gcgcggaagc gaaatcaccc gtggatggat ggttcggtga ccggggtacg gttcggtatg
     7261 tcggccccaa aaaagcacaa aaacctgctt tcagcagcgg tctatcgtgt agaagggccg
     7321 agactggaag gccgtcgcgc tctggtctat ggacccgtat cccatttcgt cagg
//
LOCUS       DAOSOR010000275         7369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10108_length_7369_cov_4.431091, whole genome shotgun sequence.
ACCESSION   DAOSOR010000275 DAOSOR010000000
VERSION     DAOSOR010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7369
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10108_length_7369_cov_4.431091"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="PK710_14550"
     CDS             complement(<1..1045)
                     /locus_tag="PK710_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10995.1"
                     /translation="MKVPPAIDSDSDDVVWALQTAAALVERNELQDALTWLRRAARAA
                     AEQDDVRSLELARIAADFADSMAPTRRVELPVDGQEADSTSRLSSVVSSAETFFEDDV
                     VTSAPPISVLQSGSPERGWVSSTPSTIKTAPPVAAMAEPTLVSPEYTQPIEIVAKKSV
                     PLPPLPPSANEVPTREPAAAAHVADAAGAVDTTWTSEQDTISLEEIPSTSVMTEGQAD
                     DIEPAVVEPSKASGFVVPPPPPMHRNRSLTSAEGKQETGARPTERPIVPPPPARDRGL
                     NKRAPSKLPPPGVKPPPLRFEEPLVVDDSPGEAAAVRFPTPPPRKMPSEEDKPTVDLD
                     LSQAEAFVDMPDDL"
     gene            1396..3426
                     /locus_tag="PK710_14555"
     CDS             1396..3426
                     /locus_tag="PK710_14555"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOT10996.1"
                     /translation="MNQVSSFIARLVACFDDVRDRDTSLIVAYAALEQYARKQVDFDR
                     LLDHVEKTESRGQSDRAPADILRQAVARWLEAISGLSPSALAYLPCELWLALTPLEKR
                     KRFGEFYTPRWLVELALQQVGWPHASDRLIDPTCGAGAFLAGAALALRQDPKRLTENA
                     RRLSGADIHPLAVTGARIACLCAVADLLEPDQPFEPNIHVADLFDPPPFCPHDVVVGN
                     PPWLRFSELPPSLRRQVADAAQHYRLVPSSSFHGGSELDTAAVCAYRMLDKHLKSEGK
                     AALVMPSTVLRSAAAASFRRFSLPDGTPLRLEHITDFGPLRVFPDAANHTTLLLFTKG
                     HPQASIIPASWTSHPRAPSYQAGLSDALQAIELVSGIARPVGKSRALACIPEPAVCLE
                     GSCSHVRGRKGVTTDLNGAYFVRILGPGSEPERLRVINDVTSRGKPVATHVFEVEQQL
                     VFPLLKGSKQIHPFRIDTPSIAILIPNRSVTRIPSEEEFSKRYPAAYAHFLWVEEQTN
                     GALRARSTYRRMLSSRDAFFSVYNVGDYTFAPFKVVWAEIAKTLVAGIASSSSLPYCS
                     TPKTIVPDHKVYFAPFEQLEPALFLCALLSSTLVRQYVDAITEKLQVGSLLDRINLPC
                     FDPNDEDHQLLVQFARQPQPIDPKQLDSYAKRVIERRSQDGNTTLLLNFNSL"
     gene            complement(3444..5732)
                     /locus_tag="PK710_14560"
     CDS             complement(3444..5732)
                     /locus_tag="PK710_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOT10997.1"
                     /translation="MSQEQQVAFDPVPQVAADLKLAPQSVAAVVRLLEEGATVPFIAR
                     YRKEVTGGLEDVAIVAIQERASYVKELEERRQVILTEIEKQGKLTDDLRQAIEGCSEK
                     ARLEDLYLPFKPKRRTRATIAKEKGLEPLSDRIWAQGDGDPAKEAVAFVNEAKGVTDV
                     EAALAGARDICAERVSEDADLRAIVRDTYMTTGMLVVSKAKEHVDAVTKFDTYASFEQ
                     AMASLPSHRFLAIRRGEQEGILRSKLRIDEQQVGDTLVTRLKVRKDNPWGKHLELAIR
                     DALTRLMMPSVESDVRVDRKLEADKAAVEVFALNLRQLLLAAPLGTKTVVGIDPGQRT
                     GCKVAVVDHTGKLIDHAVLYLVQGDRALDEARKSLRTMCKTHQPAAIAVGNGTHGRET
                     EAFVREVLVSEGLQEVFAVSVSEAGASVYSASDIARQEFPDLDVTVRGAISIARRLQD
                     PLAELVKIDPKSIGVGQYQHDVHQPMLARKLDQVVESCVNLVGVELNTASVPLLSRVA
                     GLSATVAKRIVAYRDKQGAFRSRKQLLEVTGIGPRTFEQAAGFLRIRDGEHPLDASAV
                     HPERYALVEQMAKDLGVSLPTLVGNGVWAGKINWKRYVSQDVGEPTLKDILAELAKPG
                     RDPRDSFEPPKFRDDVRTMEDLTVGMVLEGVVTNVTAFGAFVDVGVHQDGLVHVSKLA
                     DRFVRDPNEVVKVGDRIVVRVMEVDLARKRISLSARSHDHPSPPSASQEARSSDRGKP
                     HKRPEQAKSFSNHPFAKLLPKG"
     gene            complement(5897..6868)
                     /locus_tag="PK710_14565"
     CDS             complement(5897..6868)
                     /locus_tag="PK710_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10998.1"
                     /translation="MMRASRSAWIYWTILCVLMSSPALGQSAASQQQAVAKYKRGRTL
                     IEKKDYEGALQELTASYALMESPNTMLLMAHAQRELGREQQAVELYETVVKDASQRVA
                     NGEARYKKTEEDAQEWLDRLSERYGKVSIRLVNASGATVWLGREQVEVSGDSLGPLWW
                     KAGPVQVQVKDSEGRTKRVVVEVRAGTTVTQTIDFGERITEEAPREPVVSLPEKPGKS
                     IPTASWVTAGVGVAGFATFAVFGSSAKSKASELEACSPRCGEAERDVADAGKRDQRIA
                     NIGAAVGGVGLLSALGFYLLQSEEPSETVNVSLGPRGMLVQGTFRGW"
     gene            complement(6865..>7369)
                     /locus_tag="PK710_14570"
     CDS             complement(6865..>7369)
                     /locus_tag="PK710_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT10999.1"
                     /translation="PPIRSSQPSHSGPISIPSLETATPRPVSHDESSMPSLGTSPTGT
                     AFEAVPSFSKYRYLPIVLISSLVLGTLVVLIVAIISSNRGTSQPMVVLSTGSSWVSQH
                     AAPEVKSTEIFPQPVEPMTNDEDAKASEPQPTDRSVEVKPVTPPVTTPYRVPTSTKKA
                     PYLPDLP"
BASE COUNT         1489 a         2317 c         1930 g         1633 t
ORIGIN      
        1 gaagatcgtc gggcatgtcc acaaacgctt cggcctgaga tagatcgaga tcaacggtcg
       61 gcttgtcttc ctccgacggc atctttcgcg gtggtggtgt tggaaaacgg acggcagcag
      121 cttcccctgg cgagtcgtcg accacgagag gctcttcgaa tcgtaggggt ggaggcttga
      181 cgcctggagg cggcaacttg gacggcgcac gcttgttcaa cccacgatcc cgcgccggtg
      241 gcggaggcac gatgggtctt tccgtcggcc gcgccccggt ctcttgcttg ccttcggcac
      301 tcgtcaacga cctgttgcgg tgcatcgggg gcggaggcgg aaccacgaat cccgaagcct
      361 tggatggttc gacaacggcc ggttcgatgt cgtcagcttg cccctcggtc atgacgctcg
      421 tggaaggtat ctcctccaac gagatcgtat cctgttccga cgtccacgtg gtatccaccg
      481 cgcccgcggc atcggcaaca tgagccgccg cggcaggttc acgcgttggc acctcgttcg
      541 ctgaaggtgg taagggcggt aagggaactg attttttagc aacaatttca atgggttgtg
      601 tgtattccgg agaaacgagg gtgggctcgg ccatcgccgc aaccggcggc gccgtcttga
      661 tggtcgaggg cgtggaactc acccaccctc gttccggact cccgctttgc aacaccgata
      721 tcggtggcgc actcgtcacc acatcgtctt cgaaaaatgt ctctgcagaa gacaccaccg
      781 aagacaagcg cgacgtggag tcagcctctt gcccatcgac cggaagctcg acccgtcggg
      841 tgggggccat cgaatccgca aaatccgctg cgatccgggc aagctcgagc gatcgaacat
      901 cgtcttgttc cgccgcggca cgagcggcac gacgaagcca ggtcaatgca tcctgcagct
      961 cgtttcgttc tacgagcgcg gccgctgtct gcaaagccca gacgacatca tcggagtcgg
     1021 agtcgatagc aggagggact ttcaccgaaa cacctcacct ggccccggag tgtacccaat
     1081 aataggagca aagaggatgg ttccttccct ttctggggtc gaatgagaaa tgggtaatcc
     1141 tacatcggtt gaccagcgcg gtattgtcac catccttcga atcgccatga gccatcccgc
     1201 gcgatggcag atggggaaaa acaggctaga tccgacggag aagcgtttcg gattgacgat
     1261 ggggggcagc tatgaaaaca gcagagcgcc tgtcggtatt cggctcgtcc cagggaaccg
     1321 cttgcgaccc gtggcatgat ccggatatcg cgttgaccca atatttctgc catcggtacg
     1381 tccagtttca tcaacgtgaa tcaggtttct tctttcatcg cccgtctggt tgcctgcttc
     1441 gatgacgtga gggatcgtga tacatcgctc atcgttgctt atgcggcctt ggagcaatac
     1501 gcgagaaaac aagtcgattt cgatcgattg ctggaccacg tagaaaagac cgagtctcga
     1561 gggcaatcgg atcgagcgcc tgcggatatc cttcggcaag cggtggctcg atggctcgaa
     1621 gcgatttcgg ggttgtcgcc ttccgctctt gcttacttgc cctgcgaatt atggttggca
     1681 ctaacccccc tcgagaagcg caaacggttt ggtgagtttt acaccccccg ttggctggtg
     1741 gaacttgccc tccaacaagt aggctggccc cacgcttccg accgtctcat cgatcccacc
     1801 tgtggcgcag gagcctttct cgccggagcg gctctcgccc tgcgccagga tcccaaacgc
     1861 cttactgaaa acgccagacg gctctccggg gccgacatac acccgctggc cgtgaccggt
     1921 gcccgcatcg cctgcctctg cgccgtcgca gacctactcg aacccgacca acccttcgaa
     1981 cccaatatcc acgtcgccga tcttttcgat ccaccccctt tctgccccca cgacgttgtc
     2041 gtcggaaacc ctccttggct acgcttttcc gagcttccac cgtccttacg ccgtcaagtc
     2101 gccgatgcag ctcaacacta ccgtctcgtc ccctcatcgt cgtttcatgg tggcagcgag
     2161 ttggatactg cggcggtctg tgcctaccga atgctcgaca agcacttgaa atcagaggga
     2221 aaagccgcgt tggtgatgcc gagcaccgtg cttcgttccg cggccgccgc gtcgtttcgt
     2281 cgcttttcgt tgcccgatgg cacccctctg cggctcgaac acatcacgga cttcggccct
     2341 ctgcgcgtct ttcccgatgc cgccaaccat acgacactcc ttcttttcac aaagggacat
     2401 ccacaagcgt ccattatccc cgcctcgtgg acgtcccatc ctcgtgcgcc gtcgtatcaa
     2461 gctggcctgt ctgacgccct tcaagccatc gagcttgtct ctggcatcgc acgtccggtc
     2521 ggaaaaagcc gcgctctcgc ttgcatcccg gagcctgcgg tttgcctcga gggctcttgc
     2581 tcccacgttc gcgggagaaa aggcgtcacc accgatctga acggcgccta tttcgttcga
     2641 atccttggtc ccggatcgga accagaacgg cttcgggtca tcaatgatgt gacgtctcgc
     2701 ggaaaacctg tcgccaccca cgtgttcgaa gtcgaacaac aactcgtatt tcctttgctc
     2761 aaaggttcga aacaaattca tccctttcgt atcgacacac catcgatcgc cattttgatt
     2821 cccaatcgtt cggtgacccg catccctagc gaggaggagt tttcgaagcg ttatcccgct
     2881 gcctatgccc attttctgtg ggtggaggag caaacgaatg gcgcgctccg ggctcgctcc
     2941 acctaccgac gcatgctgtc ttcacgcgat gctttttttt ccgtgtacaa cgtgggtgac
     3001 tacacgtttg ctcccttcaa agtggtgtgg gcggaaatcg caaagacgtt ggtagccggc
     3061 atcgcgagtt cgagttccct accctattgc tccacgccca aaacgatcgt tccggatcat
     3121 aaggtgtact tcgctccttt tgagcaactc gaacccgccc tctttttatg tgccctgctg
     3181 tcgagcaccc tcgttcgtca gtacgtcgat gccatcacgg aaaaacttca agtaggctct
     3241 ttgctcgatc gcatcaacct tccgtgtttc gatcccaatg acgaagacca tcaactcctg
     3301 gtgcagttcg ctcgccaacc acaacccatc gatcccaagc agctcgactc gtatgcgaaa
     3361 cgggtcatcg agcgccgttc gcaggatggc aatacaactt tattattgaa tttcaatagt
     3421 ttataacaga aaggaaagta cgttcatccc ttggggagca gcttggcgaa gggatgattc
     3481 gaaaaggatt tggcctgctc ggggcgttta tggggcttgc ctcgatccga agagcgcgcc
     3541 tcttgggagg ccgatggcgg cgatggatga tcatggctcc ttgccgatag ggaaatccgc
     3601 tttcgagcga gatcgacttc catgacgcgc acgacgattc gatcgccaac cttcacgact
     3661 tcgttggggt cacgcacgaa tcgatccgcg agtttggaaa catgaacgag tccatcctga
     3721 tgaactccta cgtccacgaa agcgccaaac gcggtgacgt tcgtcacgac cccttcgagc
     3781 accatcccca ccgtaaggtc ctccatcgtt cgcacgtcat cgcggaactt cgggggttcg
     3841 aacgaatcac ggggatctcg tcctggtttg gcgagttccg caagaatatc tttcaacgtc
     3901 ggctccccga cgtcctggga aacgtacctt ttccagttga tttttcctgc ccatacgcca
     3961 ttgcccacca gggtcggcaa cgacacgcca agatctttgg ccatttgttc gaccaacgcg
     4021 tagcgctcgg ggtgtacggc actggcatcc aatggatgct caccatcacg gatccgcaaa
     4081 aatcctgcgg cttgctcgaa cgtacgtggt ccgatgcccg tcacttccaa gagttgtttt
     4141 cgcgatcgaa aggctccttg cttgtcccga tacgccacga ttcgcttcgc tacggtggcg
     4201 ctcaacccgg ctacccgaga caagagcggc acactcgccg tgttgagttc cacccccacc
     4261 aagttcacac agctttccac gacctggtcg agcttgcgag ccagcatcgg ctgatgcaca
     4321 tcgtgttggt actgacccac accaatggac ttgggatcga tcttcacgag ttccgccaag
     4381 gggtcttgta gccgacgcgc gatactaatg gcccctcgga cggtgacatc caagtctgga
     4441 aactcctgtc gtgcgatatc cgacgcggaa tacacgctcg ctccggcttc gctgacggaa
     4501 acggcaaaga cttcctgcag accttcggaa acgaggactt ctcgaacgaa cgcctcggtc
     4561 tcacgaccat gggtgccatt gcctaccgca atggcggcgg gctgatgggt cttgcacatc
     4621 gtccgcaagg atttccgcgc ctcatcgagc gctcgatccc cttgcaccaa gtacaatacc
     4681 gcgtgatcga tgagcttgcc ggtgtggtcg acgaccgcca ctttgcatcc cgtgcgttgc
     4741 cccggatcga tgcccacgac ggttttggtt ccaagcggtg cggctagcaa aagctgtcgg
     4801 aggttgaggg caaaaacctc gacagccgct ttatccgcct cgagtttgcg gtcgactcgc
     4861 acatcacttt ccaccgatgg catcatcaac cgcgtcaacg catctcgtat cgcaagctcg
     4921 aggtgctttc cccacggatt gtctttgcgc actttcaatc gcgtcacgag cgtatcgccg
     4981 acctgctgtt catcgatccg caatttggac cggagaatcc cctcttgctc cccacgacga
     5041 atcgccagaa atcggtggga aggaagcgat gccatggctt gctcgaacga cgcatacgtg
     5101 tcgaatttcg taaccgcgtc cacatgctct ttggctttcg acaccaccag catgcccgtc
     5161 gtcatatagg tgtcacgaac gatggcgcgc aggtctgcat cctcagagac tcgttccgca
     5221 caaatatcgc gcgcccccgc caacgccgcc tccacgtccg tcacaccctt ggcttcgttc
     5281 acgaacgcga ccgcctcttt cgccgggtca ccatcgccct gcgcccaaat ccgatcggac
     5341 aacggttcga gtcctttttc tttcgcgatc gtcgctcgag tccttcgctt cggtttgaaa
     5401 ggaagataaa gatcttccaa ccgcgccttt tccgaacacc cctcgatcgc ttgtcgaaga
     5461 tcgtccgtca gcttgccctg tttttcgatc tcggtgagga tgacctgcct tcgttcctcc
     5521 agctctttca catacgacgc tcgctcttgg atggccacaa tggccacatc ttcgaggcct
     5581 cccgtcacct ccttccgata gcgggcaatg aagggaactg tcgctccttc ttcgagaaga
     5641 cgaacgactg ccgccacgga ttgtggggcc aacttcaagt cggcagccac ttgcggaaca
     5701 ggatcgaagg caacttgttg ttcttggctc acggagcagc cctttacgac gactcgcctt
     5761 tttggtcgag cttgacaacg cgcgatcgtg ccgttccttg ggttcacgac tcgcgagcga
     5821 agggaatagc tcccaccatt cgctggatca ggtcgttgtg gctcgaacct gacgagctac
     5881 agatggccga cgatcgctac catccgcgaa atgttccctg taccagcata ccccgaggac
     5941 ccaacgacac gttgaccgtc tcggaaggtt cctcagactg cagaagatag aagcccagcg
     6001 cggaaagcaa tccaacgccg cccaccgccg cgccgatgtt ggcgatccgt tgatctcgct
     6061 tgcccgcgtc cgccacatct cgctccgctt caccacaccg aggcgaacaa gcttccaact
     6121 cggaagcctt gcttttggca ctggatccaa acacagcaaa cgtcgcaaac ccagcaaccc
     6181 caacgcccgc cgtcacccaa gaagccgtcg gaatcgattt cccaggcttt tccggaagcg
     6241 aaaccaccgg ttccctcggt gcttcttccg tgattcgctc cccaaaatcg atggtctgcg
     6301 tcacggttgt acccgcgcga acttccacaa caacccgttt cgttcgccct tcactgtctt
     6361 tgacttgcac ctgaaccgga cccgctttcc accataatgg tcctaacgaa tcccccgata
     6421 cttctacttg ctccctgccc aaccacaccg tcgctcccga cgcattcacc aatcggatcg
     6481 ataccttgcc ataccgctcg gataaccgat cgagccattc ctgcgcatct tcctcggttt
     6541 ttttgtaacg cgcctcaccg ttcgcaaccc tctgcgaagc gtccttgacc accgtttcgt
     6601 acaattccac ggcttgttgc tcccggccaa gctcccgctg ggcatgggcc atcagcaaca
     6661 tcgtgttggg gctttccatc aacgcatacg aagcggtcaa ctcctgcaac gctccttcat
     6721 aatccttttt ttcgatgagc gtccggcctc gcttgtactt tgcgacagcc tgttgctggg
     6781 aagccgcgga ctgccccaac gcgggtgaag acatcagaac acacagaata gtccaataaa
     6841 tccaagcaga tcgagatgct cgcatcatgg caggtccgga agatacgggg ccttctttgt
     6901 cgaagtcggc acacgataag gtgtcgttac aggaggcgtc acgggtttaa cctccaccga
     6961 tcgatccgtg ggttgtggtt cagaagcttt ggcatcctca tcgttggtca tcggttccac
     7021 gggctgcgga aatatttcag tcgactttac ctcgggtgcc gcatgctgcg atacccaaga
     7081 cgaccctgta gacagtacca ccatcggctg cgaagtgcct cgattcgaac tgattatcgc
     7141 tacaatcaaa acgacgagcg ttccgagtac caaggaagaa atcaagacaa tcggtagata
     7201 acgatacttc gagaaggatg ggacagcctc gaacgcggtt cccgtgggac tggttccgag
     7261 cgacggcatc gacgattcgt cgtgggatac tggccggggt gttgccgttt ccaacgaggg
     7321 gatggatatc ggaccggaat gagagggctg cgacgatcga atggggggg
//
LOCUS       DAOSOR010000276         7348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10139_length_7348_cov_3.939668, whole genome shotgun sequence.
ACCESSION   DAOSOR010000276 DAOSOR010000000
VERSION     DAOSOR010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7348
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10139_length_7348_cov_3.939668"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PK710_14575"
     CDS             complement(<1..487)
                     /locus_tag="PK710_14575"
                     /inference="COORDINATES: protein motif:HMM:NF036823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOT11000.1"
                     /translation="MNEAYCQLFLRETRHGILAGVKLPPPTDDLHVSPLLTEKEKEHV
                     LWLPVLRRAAWMGGRVALRLAARHFGIELDSVFSTHRGAPSLPPGLVASISHKRRIAV
                     ALLARSSTFTVGVDVEELDRPRPKIARLVLRTPESHRIENLPHAQRWPAIITTFSIKE
                     SI"
     gene            complement(573..1076)
                     /locus_tag="PK710_14580"
     CDS             complement(573..1076)
                     /locus_tag="PK710_14580"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT11001.1"
                     /translation="MKTIVAAIDFSDTTTIVIDTAVQLAHALHAEIVLFHATPPSGTW
                     LMAGLDGLTAAGMLHPVLWERQQQDILQQHLDNLITQVQKQGLTVRSHTTSAVQRNAI
                     CQALESLHPDFIVIGSHRHGSLYELLGPGICSRIVRRAPCPVVVVHSQPSASSSPAKH
                     SNTNANA"
     gene            1293..2645
                     /locus_tag="PK710_14585"
     CDS             1293..2645
                     /locus_tag="PK710_14585"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HOT11002.1"
                     /translation="MKRSFPSSFLFGTATSATQVEGHCTTSDWYDFASQPGRVNNGDA
                     PHPACDVWHRFEQDVDLIKDFGYNAHRFSIEWARIEPSPGRFDDDAIERYRRMLGALR
                     DASIAPMVTLLHFSLPQWIANQGGFLNPSFASLFERFVNRSVERLGDLVDRWVTINEP
                     NVTAALGYFVGIFPPGHRRPWQAWRAQEAMLDAHARAYRAIHERAGRCGWNAEVGVAH
                     HLRVVEPWDSSSPTDRWASDLFEQTFNRAFLNALCGLELSRAERSMLALTRTKRRDVR
                     DTHDFFGLNYYGRFKVRVGRNGTYGIPIKLEASPGAEISDVGWEVYPEGLGRFLREWS
                     ERCGRPVYVTENGIADSRDAQRASFLVRHLAQVADAMQDGVDVRGYFHWSLLDNFEWT
                     EGYEPRYGLVEMQADTLNRCPRPSAAVYGQIASKKEIDDGLWEQYGTVPERAWRAGGV
                     "
     gene            complement(2810..3976)
                     /locus_tag="PK710_14590"
     CDS             complement(2810..3976)
                     /locus_tag="PK710_14590"
                     /inference="COORDINATES: protein motif:HMM:NF014027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HOT11003.1"
                     /translation="MTVFKKEEVARDERLFRDPEEFGLEPLEPVDIRKIDDFDGLLAA
                     MARTSFGGRQVGVAADVLTSMFSDRTCKRVLTVTGAMTVGKMTLLIVELIERGLVDII
                     ITTGALQAHGMIEGLGLKHYKLDAAAPRELHDDAYLAKVGFNRVTDTIELEKNFYAAE
                     MVVEEALRDIYEPVPESERPVTLAGSDVMRSLGRVLERRFPGNRSILSTAERMGVPIF
                     VPAWTDSELFLETEIFNYRAELAGRRRLIQVASYLDWREYQDMMTSHGGSLGILTIGG
                     GVPRNWAQQLGPQLDRLQNEFGRWPVKRFAYGVRICPDPAHFGHLSGCTFAEGKSWYK
                     FLPQARTAEVLADATIVWPLLMLGVFQRLDKIAAAQRDSGTQPAAKTYPFKTRS"
     gene            4218..5786
                     /locus_tag="PK710_14595"
     CDS             4218..5786
                     /locus_tag="PK710_14595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOT11004.1"
                     /translation="MKVCTTVEMHAIEAYALSELAIPSFLLDENAAFSAGRVLRHHPE
                     FPTSVPVLIACGPGLVGAAGLALARQLHAAERQVQVLLASDTTGQQNWLDNVQTTRRY
                     GVSCNPIAAFDQLTSDLSQPCVVVDALSGAVSNDSTDVHAFIDAVNERSVPVLSLDIP
                     SGICPDTGRLTNHFVRACATLAFGLPKRGNFLSPGASYQGRLYVTTMGLPPHFLAAPD
                     VKVELVKPCELPTRVAHGHKGTFGDILFIAGAGTYYGAPGLAALASLKAGAGYARLAC
                     PASMIPALATFAPEVVFAPQRQTLEASLTRDNLSSLLSIAQAVDFVVVGPGLSLHPET
                     QGLVRDILVSLPKPLLVDGDGLSAIVDHPDLLHGRSSPTVLTPHPGEMARLLHISMRQ
                     VLDEPIEIARQAAVRFQAIIVLKGPHSIVAHPNGETSLNTSGNSGMGTAGSGDVLTGT
                     IAAMAGLGLPLRKAVQTGVFIHGFAGDLASETMGADGMTARDILFHLPEAIRIYRKSH
                     ASVLADHHGACTVI"
     gene            complement(5794..5919)
                     /locus_tag="PK710_14600"
     CDS             complement(5794..5919)
                     /locus_tag="PK710_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11005.1"
                     /translation="MVRTARMGPTVRMAPTVLTVPTARMAPTGRGSWCSSPREAS"
     gene            complement(6126..6632)
                     /locus_tag="PK710_14605"
     CDS             complement(6126..6632)
                     /locus_tag="PK710_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11006.1"
                     /translation="MKVSRRQCVGWLGATVALAPVAVASVQNGETQSEVSDCCDSLTA
                     AALLSPLVVGSSIGRWQITALGALHAGAVSVQLSGLDGQLFHIDICARDDAPGAPEPP
                     AHTDHCDLFIANEGKGNDPTVEDQGLAAMALAEVIRTHENTTDLHGLMTLRARLDLHG
                     ANVVRRCV"
     gene            complement(6756..>7348)
                     /locus_tag="PK710_14610"
     CDS             complement(6756..>7348)
                     /locus_tag="PK710_14610"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOT11007.1"
                     /translation="VLSACERTLKGLTERGASVVEIDIEGLNLARLAHLVTIVAEMAA
                     SQQAQGVQKSQYGLDTRLNFALGQALTASDYVHAQRHRAAICDYFSRIFEHLDGIVTP
                     TTACTAPIIHEDALSGESNLTLLGKLMRFVAVANLTGLPAITFPAGYDEQGMPIGLHV
                     LGRAWQEHRLLRIAHVSERLVERRSPRTYYRLLDGR"
BASE COUNT         1560 a         1940 c         2153 g         1695 t
ORIGIN      
        1 agatcgattc tttgatcgag aaggtggtga tgatggcggg ccagcgttgc gcatggggaa
       61 ggttttcgat gcgatgggac tccggggtcc gaagaacgag gcgtgcaatt ttgggtcgtg
      121 gtcggtccag ttcttcgaca tcgacaccga ccgtgaaggt ggaggagcga gcgagcagtg
      181 caacggcgat ccggcgttta tgggagatgg aagcaacgag cccaggggga agacttgggg
      241 cgccccgatg ggtggaaaac acggagtcga gttcgattcc gaaatgacga gccgcgagcc
      301 gtaatgcaac acgcccgccc atccaggccg cgcgtcgaag cacgggcagc cacaaaacat
      361 gctctttctc tttttcggtc aacaagggcg agacatgcaa gtcgtcggtc gggggtggta
      421 gcttgacacc agccaggatg ccgtggcgcg tttccctcaa aaatagttgg caatacgctt
      481 cattcatgga tcaaaacgaa gtgtcgcctc aaagcggcga gccgacgagt gggcggcatg
      541 catactgcca atgattgcca tgggggtgtt gtctatgcat tggcattggt gttggaatgc
      601 ttggccggtg acgaagatgc ggatggttgg gagtgaacga caacgacggg gcagggggct
      661 cgtcgaacga ttcgtgagca aattccgggg cctagcaatt cgtacagaga gccgtggcga
      721 tgagagccaa tcacgatgaa atccggatgg agtgattcga gggcttggca gatggcgttt
      781 cgttgaaccg cgcttgtggt atgggagcgg acggtcaacc cttgcttttg gacttgggtt
      841 atcaggttgt cgaggtgttg ctgtaaaata tcttgttgtt gtcgttccca aagaacgggg
      901 tggagcatac ctgcggcagt cagaccatcc aggccggcca tgagccaggt tccagaaggg
      961 ggagtggcat ggaagagaac gatttccgca tgcaacgcgt gggcgagttg tacggctgta
     1021 tcgatgacga tggttgtggt gtctgaaaag tcgatggcgg caacgatggt tttcatggtc
     1081 gcgtcccctt ttcattcatg acacggcagg ggtcgtatcg aaaggtggag cacccaatcc
     1141 gtattgtggc acggggtgat gaaaaatgcc cagaatcgag gttgaattcg tgggatggga
     1201 gggttggctt tcaggattgg gtttacaacg tctcgttgtt ggcgcagcgt ttgcggtgac
     1261 gcagtggatg cgatgcgagc ggaatgcgag tgatgaaacg ttcttttcct tcctcgtttt
     1321 tatttggaac ggcaacctct gccacacagg tggaagggca ttgcacgacg agtgattggt
     1381 atgattttgc ctcgcaaccg ggccgcgtga acaatgggga tgctccccat ccggcttgtg
     1441 acgtttggca tcgattcgag caagatgttg atttaattaa ggattttgga tataacgcac
     1501 atcgtttttc gatcgagtgg gcacgtatcg agccgtcgcc gggtcgattc gacgacgacg
     1561 cgatcgaacg atatcggagg atgctgggag cgcttcgtga tgcatcgatc gcgccgatgg
     1621 tgacgctgtt gcacttttcg ctcccgcagt ggattgcgaa ccaaggaggg tttctcaacc
     1681 catcgttcgc gtcgcttttc gagcgcttcg tgaaccgttc cgtcgaacgt ttgggggact
     1741 tggtggaccg ttgggtgacc atcaacgaac cgaatgtcac ggcggctctt ggatatttcg
     1801 tcggaatttt tccgccagga caccgtcgtc cttggcaagc ctggcgagcg caggaagcca
     1861 tgctcgatgc gcatgcccga gcgtatagag ccatccacga gcgcgcgggg cgttgtgggt
     1921 ggaatgccga ggtaggggtg gcgcatcacc ttcgtgtggt ggaaccttgg gatagcagca
     1981 gccccaccga tcgatgggca agcgacttgt tcgagcaaac gttcaatcga gcgtttctca
     2041 atgcgctttg cgggctcgag ctttctcgtg ctgagcgaag catgctggcg ctcacaagga
     2101 caaagcgtcg tgatgtgcgt gacacgcacg actttttcgg actcaattat tacggacggt
     2161 tcaaagtacg agtggggcga aacggtacct atggtattcc gatcaagctc gaggcctcgc
     2221 cgggggcgga aatatcggat gtcggttggg aggtgtatcc tgagggcctg ggccggtttt
     2281 tgcgtgaatg gtcggagcgc tgtgggcggc ctgtctatgt gacggaaaat gggattgcgg
     2341 attcgcgtga tgcgcagcgc gcgagttttt tggtgcgaca cctggcgcag gtggctgatg
     2401 cgatgcaaga tggggtggat gtgcgggggt acttccactg gtctctcctt gataacttcg
     2461 aatggacaga ggggtatgaa ccacgatatg gacttgtcga gatgcaggcc gatacgctga
     2521 accgttgtcc tcgtccgagc gcagccgtat atggacaaat cgcatcgaaa aaagaaatcg
     2581 acgatggtct atgggaacaa tacggtaccg tgcccgaaag agcttggaga gcgggtggag
     2641 tataacgagc cttgggagag gcgaatggct ctggggaagg cagccgatag gctcggtctc
     2701 gtctagatta caatgtaact gtggcaacgg acaatgagga taggcgctac gtggttattg
     2761 ttcgatggaa atgagcgaat cgatagggcg tattcggaga ggagatgatt taggagcgtg
     2821 ttttgaatgg ataggttttt gcggcaggtt gggtcccgga gtcacgctgc gccgcggcga
     2881 tcttatccaa ccgttgaaaa acgcccagca tcaacaaagg ccaaacgatc gtggcgtccg
     2941 cgagcacctc cgccgtccgc gcctggggca aaaacttgta ccagcttttg ccttcggcaa
     3001 acgtgcatcc ggaaagatgt ccaaaatgcg cgggatcggg gcagatgcgc accccatagg
     3061 cgaaacgttt gacgggccaa cgaccgaatt cgttttgcaa tcggtcgagt tggggaccaa
     3121 gttgttgcgc ccaattgcgg ggaactccac ctccaatggt gagaatcccc aacgagcctc
     3181 cgtgagaagt catcatgtct tggtattcgc gccaatccag ataggaagcc acctggatca
     3241 accggcgacg tcccgcgagt tcggctcgat aattgaatat ttcagtttcg agaaaaagct
     3301 cggaatccgt ccacgctggg acaaatatgg gaactcccat gcgctcggcg gtcgatagaa
     3361 tcgagcgatt gccgggaaag cgacgctcca acactcttcc gaggcttcgc atgacatccg
     3421 agcccgccaa agtcacgggg cgttctgatt ccggtaccgg ctcgtaaata tcccgaagcg
     3481 cttcctccac caccatctct gccgcataaa agtttttttc taattcaata gtatcggtga
     3541 cgcggttgaa acctactttg gcaagatagg catcatcatg aagctcccgg ggggccgcgg
     3601 catctagctt gtaatgcttg agccccaacc cctcgatcat gccgtgcgcc tgcaaagctc
     3661 ctgtcgtgat gatgatgtcg acgagtcccc gctctatcag ttcgacgatg agcaatgtca
     3721 tctttccaac cgtcatcgca cccgtaaccg tcaacacccg cttacacgtc cggtctgaaa
     3781 acatcgatgt tagcacatcg gccgcaaccc ctacttgcct acccccaaac gatgttcgag
     3841 ccatggcggc cagcaaccca tcgaaatcat caatctttcg aatatccacc ggctccagag
     3901 gctctaaccc aaattcttcg ggatctcgaa ataatcgctc gtcccttgcg acctcttctt
     3961 tcttgaatac cgtcacaccg tactcctcgc tgcatttccc atcagattgt gttacccgct
     4021 gtttcccatc ggggcaaccc tttcaggcca atttgattct tgaaatatgt aaactaagta
     4081 aataatgata aaaatttatt cgtttattat gtataatatg gatattgtaa aatagtagga
     4141 tcgtgtcaat tattatgtgt cgtttctttc gacaactcac gctggaagcc gtccgtggac
     4201 tcacgcagga ttagccaatg aaagtctgca caaccgtcga gatgcatgcg atcgaggcgt
     4261 acgccttgtc ggagttggca attccatcct tcctccttga cgaaaacgcg gcgttttcgg
     4321 ccggtcgagt tctgcgtcac catcccgagt tcccaacttc tgttcccgtg ttgattgctt
     4381 gtggcccggg acttgtcggg gccgcaggcc tcgcgcttgc tcgacagctt catgcggcgg
     4441 aaaggcaggt tcaggtcctg ctcgcgagtg acaccacagg acagcaaaat tggttggaca
     4501 atgtccaaac cacccgccgc tacggtgttt cgtgcaaccc catcgccgcc ttcgaccaac
     4561 tcacgtcaga cttgtcccaa ccttgtgtcg tcgtcgatgc gctgagcgga gcggtttcca
     4621 atgattcaac cgacgtgcat gcattcatcg atgccgtcaa cgaacgatcg gttcctgtct
     4681 tgtcgttgga cattccttcc ggcatttgcc cggataccgg caggctgaca aatcacttcg
     4741 tacgtgcctg cgcaaccttg gctttcggct tgcccaaacg aggcaatttc ctgtcgccgg
     4801 gcgcgagtta tcagggccgg ttgtatgtca cgacgatggg tcttccgccg cactttcttg
     4861 ccgcacccga tgtgaaggtg gaattggtaa aaccttgcga gttgcctacg cgtgtggcgc
     4921 acggtcacaa gggaacattt ggcgacatct tgttcatcgc aggcgcggga acgtattacg
     4981 gcgcacctgg attggcagct ttggcatcgt tgaaagcagg ggcgggttat gctcgcctcg
     5041 cttgcccggc ttccatgatc cctgctcttg ccacctttgc cccagaggtc gtttttgctc
     5101 cccaacgaca aacccttgag gcttcgttga cgcgcgataa cctatcgtcg ctgctttcta
     5161 ttgcacaagc ggtcgatttc gtggtggttg gaccaggctt gtcgttgcat cctgaaaccc
     5221 aaggtttggt tcgcgatatt ctcgtttctt taccgaaacc gcttttggtc gacggggatg
     5281 ggttgagcgc gatcgtcgat catcctgatt tgcttcacgg gcggtcgtcc ccgaccgtat
     5341 tgacaccgca tccgggtgaa atggcacgct tacttcatat ctcgatgcgg caagtgctcg
     5401 atgaaccgat cgagatcgct cgtcaagccg cggttcggtt tcaagccatc attgtcttga
     5461 aaggccctca tagtatcgtt gctcacccga atggagaaac gagcctgaac acaagcggca
     5521 attccggcat gggcacggcg gggtcgggag atgtgcttac gggcacgatc gccgcgatgg
     5581 ccgggttggg attgcccttg cggaaggcag tgcaaacggg cgtttttatt catggttttg
     5641 ctggcgatct cgcatcggaa acgatgggag cagatggcat gactgccaga gacattctat
     5701 ttcatttgcc ggaagcgatt cgcatctatc gcaaatcgca tgcatcggta ttggccgacc
     5761 accatggtgc atgcacggtg atatgaggct tcgttaggat gcttcgcgcg gggaactgca
     5821 ccagctacca cgacccgtgg gagccatgcg agccgtggga accgtgagaa ccgtgggagc
     5881 catgcgaacc gtgggaccca tgcgagccgt gcgaaccatg agaagcatga gagcagtgct
     5941 gcgcgggaaa cgtcggatcg tttggatccc ccataacctc tccgttgcat gcatcgcgcg
     6001 cgcggtcgat gctccggttt tcgtcctcag aaagcatgcc ccggggacgt ctttcgtacc
     6061 gggggggagc cttgcgttgt gaagccatct cgttcctcca aagcttgtct tcgaccgttc
     6121 cgacgttata cgcacctacg cacgacgtta gcaccgtgaa gatcgagccg ggccctgagg
     6181 gtcatcagac catgtaaatc cgtggtattt tcatgggttc gaatgacttc cgccaatgcc
     6241 atggccgcca atccctggtc ctcgacggta gggtcattgc ccttgccttc gtttgcgatg
     6301 aatagatcac aatgatcggt gtgggccggg ggctcgggag ccccaggagc atcatctctt
     6361 gcgcagatat cgatatgaaa aagctgcccg tccaatccag agagctgaac cgaaaccgcg
     6421 ccggcatgca gagcgccaag tgccgttatt tgccaccgac caatagaaga ccccaccacg
     6481 agcggcgaaa gcaacgccgc cgctgttagc gaatcgcaac aatcgctgac ctctgattgg
     6541 gtttctccgt tctggacaga ggctacggcg accggtgcga gagctacggt tgctcccaac
     6601 caccccacgc attgtcgacg agaaaccttc atgatgaatg actcctgcga aatgttcgcc
     6661 aaacccgttc tgtcgtgatg gcgttccttc aagaataaca ccgcgcctgt aggtggggca
     6721 agccgtcttt cccttgatta cctgatttgc tgcgcctaac ggccgtcaag caaccgatag
     6781 tatgtccgtg ggcttcggcg ttcgactaac ctttccgata cgtgtgcgat tcgcaacagc
     6841 cgatgctcct gccacgctcg tcccagcacg tgcagtccaa tcggcatgcc ttgttcgtcg
     6901 tagcccgcgg gaaatgtgat ggccggcagc ccggtcaggt tggccaccgc aacgaagcgc
     6961 atcaatttgc cgaggagcgt caggttcgat tcccccgata gagcgtcttc gtgaattatg
     7021 ggtgccgtgc aggctgtcgt gggagtcacg atgccgtcta aatgctcgaa aatacgcgaa
     7081 aaataatcgc aaatcgctgc ccgatgcctc tgcgcatgaa cgtagtcgga tgccgtcagt
     7141 gcttgaccaa gggcaaagtt gagacgggtg tccaacccat actgactctt ctgaacgccc
     7201 tgagcctgtt gggaagctgc catctcagcc acaatcgtca ccaggtgggc gagcctcgcc
     7261 aagttcaacc cttcgatgtc aatctcaacc accgacgccc cccgttccgt caaccctttc
     7321 agtgttcttt cgcatgccga tagcactt
//
LOCUS       DAOSOR010000277         7326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10173_length_7326_cov_4.469261, whole genome shotgun sequence.
ACCESSION   DAOSOR010000277 DAOSOR010000000
VERSION     DAOSOR010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7326
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10173_length_7326_cov_4.469261"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1723
                     /locus_tag="PK710_14615"
     CDS             <1..1723
                     /locus_tag="PK710_14615"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOT11008.1"
                     /translation="AGVQRGDRVAILSTTREEWVVADLAILMAGAVCVPVYPTLTAEE
                     TAYIIENSGSTVIFVENPQQFETLTTRPASTVLAQLKAIVVFDCQSLRDIPHHESQRS
                     LTCFEVSQLVHVPSQVLADFVSLGETLLDDPEKARQLDERISNVSGDDLASIVYTAGA
                     VGRPKGVMLTHANFAFEIRTARETLEVSSRDEQLLFLPLAHIMGKLLYVMHYEIGGVI
                     SFAENMLRAVDNAAEINPTFFGSVPRVFEKYYAVTEKKAREEGKVKERLYRWALDVGH
                     RTAKLRRKGITPSLPLVIEHRYADKLVLSRIRSRFGKGLRFAISGGAPLAAELAEWFE
                     AIGITILEGYGLTETTGASNINRPYRSKFGTVGPPLNGVKNRIADDGEILLRGDNLMK
                     GYWNDPEATAEAFHEEGWLHTGDIGYIDADGMLKITDRKKDLIITAAGKNVAPQNIEN
                     FLRQSPWIAHGVVIGDGQRYLTALISLNHQALTRWAAENGRPSDPDSLVMDPEVRAMI
                     QLDIDSINHRLARYETIKDFALLADQFSIASGELTPTGKIKRNVIAKRYASTIEQLYA
                     KGGDPST"
     gene            complement(1816..2664)
                     /locus_tag="PK710_14620"
     CDS             complement(1816..2664)
                     /locus_tag="PK710_14620"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOT11009.1"
                     /translation="MSIESERLEDELRFIVCAFREGLLWQRTCGASGLPAAPPSRKNT
                     APAFDAPIRNEQQGKASGGVESSKGSSSSIDSSPALPVADRLNLSVEQRLARLSDVAT
                     QIKTCERCGLCRGRKNVVYGTGAIDVDIMIVGEGPGVEEDLQGIPFVGKAGQLLDKML
                     AAMGYARSQVYIGNVVKCRPPNNRVPEDDEIGACMPYLREQVELVQPKVMVAMGGTAV
                     RGLFGLGGITRVRGRWKLYQGKIPVMPTFHPAYLLRNESAKREVWDDLKQVLRHLNRE
                     VPKREK"
     gene            complement(2851..3651)
                     /gene="cobA"
                     /locus_tag="PK710_14625"
     CDS             complement(2851..3651)
                     /gene="cobA"
                     /locus_tag="PK710_14625"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOT11010.1"
                     /translation="MSEREVLGVVHLVGAGPGAADLITVRGHRLVMSCDALVYDHLVA
                     AELVEASQAKLKIYVGKVAGQHAMPQAKINALLVDLATRRGGPSQIVRLKGGDPFVFG
                     RGGEEAFACLERGIPFSVVPGVSSGIAAPAAVGVPLTHRDFTRGVMFITGHARGDGDL
                     DLPWEALVSSRFTLVFFMGVSTIERIASELIGHGLDASTPALVVQEATTPSQRHVTAE
                     LSDVANAVRIAEIKAPALLIVGQVAGLAPQLEGRRLPYAPSKPSSHRL"
     gene            complement(3648..4355)
                     /locus_tag="PK710_14630"
     CDS             complement(3648..4355)
                     /locus_tag="PK710_14630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HOT11011.1"
                     /translation="MRTVHTRAPSVRERRLSKLFPLFLSLANAPVLVVGAGKIAKHKA
                     QSLLRCDALVTMVAPDACEEVRAMALAGKVVFYARAFREQDVDGKRLVIAATGDATIN
                     ETVMRRCRALGVWVNVVDDPSRCDFHIPAVVERGKVQVAISTGGASPALSRMLKEQLG
                     GHLEPSLGAYVELVEQARGRIKQLMSDRGYEERRMANEAVLAAGARERLAAGDEQGAR
                     RLIDEVVEEIRVQKVEG"
     gene            complement(4584..6245)
                     /locus_tag="PK710_14635"
     CDS             complement(4584..6245)
                     /locus_tag="PK710_14635"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT11012.1"
                     /translation="MKSDYADLLVLGASAAGLKCAARYRRLCPTARIVVLDAQEEISV
                     GACGLPYVISGDIDDPDALRQTPFDVLRDEHYFRVYKRIEVSTGIRAESIDTKQREVR
                     ALRLADGSNVSFRYERLLLAMGAAPKSLAGVRVGSERVRFVKTMDDARTIRALLEKGK
                     VESAAIVGAGFIGCEMAEAFRSMWGCEVTLIEAADQILPGMLDADLASVVQRELESNS
                     VRVLLRSAVLGIEEGQGVTIRLPQESIEVDLVVVGIGVSPRTELALEAGIEVGSCGGI
                     VVDERMRTNVPNVYAAGDCVECRSVVTGRAMLATLGSLANRQGRVAADHMAGKVSRFG
                     PVARSAVVKVMDVHAAATGMTHGEAVRAGMRVCSVWGTFGDRAHYYPDEQRWFGRMVW
                     ERATSRLVGLQVVGRGDVTRSVDVFSSVLLRGGQVADLLDLEFAYAPPFASALDPLHH
                     LAAMAMAHEQDGIEALGPEEPVIGTCVDLRSVNESKAAPSEEAAMHVPMQELWEREAE
                     LPEGDLSLLCERGPRSMDAVRGLVGKRAARVVYLAGGQGFRSKKL"
     gene            complement(6312..>7326)
                     /locus_tag="PK710_14640"
     CDS             complement(6312..>7326)
                     /locus_tag="PK710_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11013.1"
                     /translation="EHSHRGGSGPADRENVDSFLRAQHALGEWTSHGCETAVHLLENA
                     HQISPRDPLLMAWLALARFRSWYLLVHASEGVATEGLRLSRLALDINENLGEAHLGLS
                     IYALYNANWIVAARRAEEAIRCNPALSEAHWIIGVLHCAANRVEQGLGMIDLALRLEP
                     RNLAALWDAAYVEALCGHRARAAEYLDKADQVYLNHPETLLARIRIGLWMKERMMIAW
                     ARENMASVGGVATAFQSAAIRMFLEPEPDEEVGTLTALAAGEDTPLAARARIMQLVAE
                     RMAIGGMVDDAWSALRAASAHAIDAIWFMRCPVLERMASMPAFLSLRSHIAARAAQVF
                     EAP"
BASE COUNT         1675 a         2544 c         1700 g         1407 t
ORIGIN      
        1 tgcaggcgtg caacggggcg acagggtcgc catcctgtcc accacacgcg aagaatgggt
       61 cgtcgccgac ttggcaattc tcatggcagg cgcggtttgt gtccccgtct accccacgct
      121 caccgcggaa gaaaccgcct acatcatcga aaacagcggt tcaaccgtga ttttcgtcga
      181 aaacccacag cagttcgaaa cactcaccac gcgccccgct tccaccgtac tcgctcaact
      241 caaagccatc gtcgttttcg actgccagtc cttgcgcgac atccctcatc acgaaagcca
      301 gcgctcgttg acttgtttcg aggtttccca actcgttcac gtcccttcgc aggtccttgc
      361 ggacttcgtt tccctgggtg aaaccctgct cgacgatccg gaaaaagcca ggcaactcga
      421 tgaacgtatc agcaacgtat cgggcgatga cctggccagt atcgtttaca cggctggcgc
      481 ggtggggcgt cccaagggcg tgatgttgac gcatgccaac ttcgcctttg aaattcgtac
      541 ggcccgtgaa acgctcgaag tctcttctcg cgacgagcaa ctgctatttc ttcctcttgc
      601 ccacatcatg ggcaagctgt tgtatgtgat gcactacgag atcggcggcg tgatctcgtt
      661 tgcggaaaac atgctgcgcg cggtggataa cgctgcggaa atcaatccca ccttttttgg
      721 ctccgttccg cgcgtcttcg agaaatacta tgccgtgacg gaaaagaaag cccgcgagga
      781 aggcaaagtc aaagagcgcc tctatcgctg ggcgttggat gtggggcatc gcacggcgaa
      841 actccgacgc aaaggaatta cccctagcct tcctctcgtc atcgagcatc gatacgccga
      901 caaactcgtg ttgtcacgta ttcgctcgcg cttcggaaaa ggactccgat ttgccatctc
      961 cggcggcgcc ccgttggccg ccgagcttgc cgaatggttc gaagccatcg gtatcaccat
     1021 cctcgaaggc tacgggctta ccgaaactac cggcgcatcc aatatcaatc gtccttatcg
     1081 ctccaaattc ggcaccgtgg gcccccccct caacggtgtc aaaaatcgaa tcgccgacga
     1141 tggagaaatt ctgttgcgcg gcgacaactt gatgaaaggc tattggaacg accccgaggc
     1201 caccgcggaa gcattccacg aggaaggctg gcttcatacg ggtgatattg gctacatcga
     1261 tgccgatgga atgctgaaaa ttaccgatcg aaaaaaagat ctgatcatca ccgcggcagg
     1321 caaaaatgtc gccccgcaaa acatcgaaaa ctttctccgc caatcaccat ggatcgccca
     1381 cggcgtggtc attggcgacg gccaacgata cctcaccgca ttgatttccc tcaatcatca
     1441 ggccttgacc cgatgggccg cggaaaacgg gcgtccaagt gaccccgata gcctcgtgat
     1501 ggaccctgag gttcgagcga tgattcaact cgatatcgac agcatcaacc atcggctcgc
     1561 acgatacgag accatcaaag acttcgctct gcttgccgat cagttttcca tcgccagtgg
     1621 cgaattgacc cccaccggaa aaatcaaacg caacgtcatc gccaaacgat acgcttccac
     1681 catcgaacag ctttacgcaa aaggcggcga tccatcgact tgaattccat cgcaaaggcc
     1741 tcgcgatacc cgcttccccc ctacgaaaat gaatgatcac ggtacgctcg aaccgtacat
     1801 tcctccgccc tgctatcact tttcccgctt gggtacttca cgattcaaat gacgaaggac
     1861 ctgtttcaga tcgtcccaga cctcacgctt cgcagactca ttgcgaagca aatacgccgg
     1921 atgaaatgtc ggcatcacag ggatttttcc ttgatacagc ttccatcggc cccgcacccg
     1981 cgtgatgccc cccaacccaa ataatccacg caccgccgtc ccgcccatcg ccaccatgac
     2041 cttgggctgc accaactcca cttgttcacg aaggtacggc atgcacgctc caatctcatc
     2101 gtcttccgga acccgattgt tgggcggtcg acacttcacc acatttccta tgtacacttg
     2161 ggatcgcgca taccccatcg ccgcgagcat cttgtcgaga agctggcccg ctttgcccac
     2221 gaaaggaatc ccttgcaggt cttcctcaac cccaggccct tctcccacga tcatgatgtc
     2281 cacatcgatg gcccccgtcc cgtacacgac attttttcga ccccggcaca gaccacaccg
     2341 ctcgcaggtc ttgatctgag tcgccacatc gctcagtctc gccagccgct gctccacgga
     2401 taagttcagt cgatccgcaa caggcaaagc cggagacgaa tcgatcgacg aagacgagcc
     2461 cttcgacgat tcgacgccgc cggacgcctt cccttgctgc tcgttgcgaa tcggtgcatc
     2521 gaacgccgga gccgtgtttt ttcttgatgg tggggccgca ggcaatccac tcgccccaca
     2581 cgttcgttgc cacaacaatc cttcccgaaa tgcacaaacg atgaaccgaa gctcgtcctc
     2641 cagccgctcg gattcaatac tcatccttca tgcatgcgat ggatgctcgt cgatctcaag
     2701 acccaaccga ccgatcatga ccaaacgaca cgatcgaacg taattcatca cgccatcacg
     2761 agaccctgcc ggaacatctt tcgtcccgaa accgcgggag caaacgacca ggctgccaaa
     2821 aaactgatgt cgaacggtat ggcaatccgc tcagagccga tggctcgacg gtttcgaggg
     2881 agcataaggc aagcgcctgc cctcgagttg cggtgctaac cccgccacct gcccgacaat
     2941 caacaatgct ggagctttga tttccgcgat tcgaaccgca ttggccacat cggaaagctc
     3001 cgccgtgacg tgccgttgcg agggagtcgt cgcttcctga actaccaacg cgggcgtgct
     3061 ggcatccaaa ccatgcccta tcagctcgga ggcaattcgc tcgatggtcg acacgcccat
     3121 gaaaaatacc agcgtaaatc gcgagcttac caatgcttcc cacggcaaat ccaaatcccc
     3181 atcgcccctc gcatgacccg tgatgaacat gactccccga gtaaaatccc tgtgcgtaag
     3241 cggcaccccc acggccgcgg gagccgcaat tcccgaactt acccccggca ccacggaaaa
     3301 gggaatccca cgctccaaac acgcaaacgc ctcttctcct cctcgcccga atacgaatgg
     3361 atcgccccct ttcaaccgaa cgatttgcga tggacctcct cgacgagtcg ccaagtccac
     3421 aagcaatgca ttgatttttg cctgtggcat ggcgtgttgc cccgctacct tgcccacgta
     3481 aatcttcagc ttcgcctgac tcgcctccac gagttctgcc gccacaagat ggtcataaac
     3541 caacgcatca caggacatca cgagacgatg accacgcacc gtgatgagat cggccgctcc
     3601 cggccccgca cccacaaggt ggaccacacc aagtacttca cgctcactca tccttccacc
     3661 ttctgaactc ggatctcctc cactacctca tcgatcaatc gtctcgctcc ctgctcatct
     3721 cccgccgcca gtcgctcgcg agcccccgcc gccaacaccg cttcattcgc catccgacgc
     3781 tcttcatatc cccggtccga catgagctgc ttgattcgtc ctcgggcttg ctccaccaat
     3841 tccacatacg cgcccaacga aggctcgaga tgcccgccaa gttgctcttt caacatccgt
     3901 gacagcgcgg gagatgcccc ccctgtcgag atcgccactt gaaccttccc gcgctccacc
     3961 acggccggaa tatgaaaatc gcaccgcgac ggatcgtcca cgacattgac ccatacgccc
     4021 aacgcccgac aacgacgcat caccgtttca ttgatcgtcg catctcccgt cgccgcaatg
     4081 acaagacgct tgccgtccac atcttgctca cgaaaggcgc gcgcataaaa aacgactttg
     4141 ccggccagtg ccatcgccct gacctcttcg cacgcatccg gtgccaccat cgtcacgagg
     4201 gcgtcgcacc gcagaagcga ttgggccttg tgtttcgcaa tcttccccgc gccaaccacc
     4261 agcacggggg cgtttgcaag actcagaaat aggggaaata gcttcgacaa tcgacgctct
     4321 cttacgcttg gtgctctcgt gtggaccgtt cgcacgaacg tgtcaactcc tatgtccttt
     4381 gccgtccatc caagcccacg catgggcccg ctgcaccact cgggtggctt tggcgcatcg
     4441 tccaatcgcc gccgcatccc tacccgtgct tccgcgacct atttgccttc ccacgcgcgc
     4501 aatgcatcga ttggaaccac cctccgctcg atccccctgc cacggcgcag agcggcatta
     4561 taattgtaac atattgaatt aaatcataat tttttcgatc tgaacccctg gcctcccgcc
     4621 agatacacca cgcgagccgc ccgtttccca accagccccc gaacggcatc catcgaccgc
     4681 ggccccctct cacacaataa gctcaaatcc ccctcgggaa gttccgcctc acgctcccaa
     4741 agttcctgca tcggcacgtg catggccgct tcttcactcg gggcagcttt ggattcgttc
     4801 acgctccgaa ggtcgacgca tgttccgatc acgggctcct caggccccaa agcctcgatc
     4861 ccatcctgct cgtgcgccat cgccatggcc gcaagatgat gaagaggatc gagtgccgaa
     4921 gcaaaaggcg gcgcataggc aaactccaag tccaacaggt ccgccacctg ccctcctcgt
     4981 aacaacacac tggaaaatac gtcgacggac cttgttacat cgcctcgccc caccacttgc
     5041 aatccaacca atctgcttgt ggcacgctcc cataccatgc gcccaaacca ccgctgctcg
     5101 tcgggataat aatgggcccg atcgccaaag gtcccccaaa cactgcatac acgcattccc
     5161 gcacgcacgg cttccccatg cgtcattccc gtcgcggccg catgcacatc catcaccttg
     5221 actacggcgc tcctcgccac cgggccaaaa cggctgacct tgcccgccat gtgatcggca
     5281 gccacgcgcc cttgtcgatt cgccaacgac cccaacgtgg caagcatggc ccgacccgtc
     5341 accaccgaac gacattccac acaatcgcca gccgcataga cattcggtac gttcgtgcgc
     5401 atccgttcgt cgaccacaat gcccccgcag gagccaacct cgattcccgc ttccaacgca
     5461 agctccgttc gaggcgaaac cccaatgcct accaccacca aatccacctc gatggactcc
     5521 tgcggcaatc gaatcgtgac gccttgccct tcctcgatcc ccaggaccgc ggaacgcaac
     5581 aacacgcgca ccgagttgga ttcaagctct cgctgcacca ccgatgccaa atcggcatcc
     5641 aacatgcccg gaagaatctg gtccgcggct tcgatgagcg tgacctcgca tccccacatc
     5701 gaacgaaagg cttctgccat ctcgcaacca atgaaacccg ctcccacgat cgccgccgac
     5761 tcgacttttc ctttttcgag caaggcacga atcgttcgcg catcgtccat cgttttcaca
     5821 aaccgcaccc gttcgcttcc tacacgcacg cccgcaagcg atttcggagc cgcccccatc
     5881 gccaacaaca ggcgctcgta acgaaaagac acgtttgatc catccgcaag acgcaaagcc
     5941 cgaacctcac gctgcttcgt gtcgatgctt tcggcccgaa tccccgtgct cacctcaatc
     6001 cttttataaa cgcgaaagta atgttcatca cgcaacacat cgaatggcgt ctgacgcaac
     6061 gcgtccggat cgtcgatatc gccggagatc acatacggca gcccgcaggc tcccacggag
     6121 atttcctcct gcgcatccaa aacgacaatt cgtgcggtag ggcaaagacg ccgatagcga
     6181 gctgcacact tgagcccggc ggcggaagct ccaagaacca aaaggtccgc ataatctgac
     6241 ttcatgatga cacctaactc gaacgaccga gtgttcgcgg tcgccggggc agtatacctc
     6301 cccgccacgc gctagggagc ctcgaagacc tgggccgctc gtgcggcaat atgcgaccgt
     6361 aaagaaagga aggcaggcat cgaggccatg cgctcgagca ccggacaacg catgaaccaa
     6421 atggcgtcga tggcatgagc cgatgctgca cgaagtgcgc tccaggcatc atcgaccatg
     6481 ccgccaatgg ccatgcgctc ggcaacgagc tgcatgatgc gagcacgtgc tgccaaaggc
     6541 gtgtcctcac ctgccgcaag cgcggtcagg gtgcccactt cctcatcggg ctccggttcg
     6601 aggaacatac gaatcgccgc ggattgaaag gccgtggcca caccacccac cgaggccatg
     6661 ttctcgcgcg cccacgcaat catcatgcgc tccttcatcc atagcccaat acggatacgc
     6721 gcaagcaatg tctctggatg attcaaatac acttgatcgg ccttatccaa atactcagcc
     6781 gcacgagccc tatgtccaca aagggcctct acgtaagccg catcccataa ggccgccaag
     6841 ttgcgaggct ccaagcgaag cgctaaatcg atcatcccga gcccttgttc cacgcgattg
     6901 gcggcacaat gcaaaacgcc gatgatccaa tgcgcctcgg aaagagccgg gttacaacga
     6961 atcgcctcct ccgcacgccg cgccgccacg atccaatttg cgttatataa cgcatagata
     7021 ctgagcccca ggtgcgcttc gccgagattc tcattgatgt ccaatgccaa cctcgaaagt
     7081 cgaagcccct ccgtggccac accttcggaa gcgtgaacga gcaagtacca cgaacgaaat
     7141 ctcgccagcg ccaaccaagc catcaacaaa ggatcccgcg gactgatctg atgcgcgttt
     7201 tccaacaaat gcacggcagt ttcacaccca tgcgaagtcc actcccccag cgcgtgttgc
     7261 gctctcaaaa acgaatcgac attctcccga tccgccggac cactgccccc gcgatgacta
     7321 tgctcc
//
LOCUS       DAOSOR010000278         7303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10212_length_7303_cov_4.399696, whole genome shotgun sequence.
ACCESSION   DAOSOR010000278 DAOSOR010000000
VERSION     DAOSOR010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7303
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10212_length_7303_cov_4.399696"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PK710_14645"
     CDS             <1..257
                     /locus_tag="PK710_14645"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOT11014.1"
                     /translation="AVCAGSCGSLVELAVSKGVDLYLTGEMSHHNAFRAASRNMTVVC
                     VLHSHSERLSLQRLKKRLQHALPNLDVMVSQTDNDPFDIC"
     gene            289..1191
                     /locus_tag="PK710_14650"
     CDS             289..1191
                     /locus_tag="PK710_14650"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOT11015.1"
                     /translation="MPMTHAGGNVPIRAVFGGLTDVGHKREHNEDHILVRVDLGLFIL
                     ADGMGGHNAGDVASKLATSSLVSFFEATRGQTYFGIPPEGYDHLDVDGLRLLFGIIKA
                     NEDVYRVSAAAGAHHGMGSTIVATYITRDGTVHIGHVGDSRCYRISDGEIEQLTHDHS
                     FVNDVLRAKPDMDPEQLAHLPQNIITRALGMREEVEPSIRSEPTIPGDVYLLCSDGLS
                     GQVSREEIHRIVGTGQNPQQACQMLVDASNAAGGKDNVSVIIIRVDPAQPRIPAPSVA
                     VCRRCGSPNTPGVATCQRCGSPMH"
     gene            complement(1240..4944)
                     /gene="hrpA"
                     /locus_tag="PK710_14655"
     CDS             complement(1240..4944)
                     /gene="hrpA"
                     /locus_tag="PK710_14655"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HOT11016.1"
                     /translation="MASHSQSPVHFPPELPISGRVQDIARAIDQHPFVIVCGETGSGK
                     TTQIPKICLAMGRGLRARIGCTQPRRIAATSVASRVADELGVELGHEVGFKIRFSDRT
                     SPDTYVKFVTDGILLAELRSDPWLNGYDTIIVDEAHERSLNIDFLLGCLKRIAPKRPD
                     LRVIISSATLEVDRFAAYFADAPILAISGRTYPVEVVYHPPASDCDLVDAVADAVDEI
                     TELGPQRDVLIFLPGEREIHEVFSILIARDYPDTVVLPLYGRMPQSEQHKVFQSLRQR
                     RIVLATNVAETSLTIPGIAYVVDSGLARINRYNPRNGMTQLQVERISQASANQRTGRA
                     GRMRSGVCYRLYSEEDFMSRPAFTMPEVQRVGLAGVILHMKTLGLGQVDAFPFLDPPS
                     KRAISDGYRVLEELGALDAQGEPNELAFQLSHFPIDPRLAKMLLAGQREGCLREMLVV
                     ASSLSIQDPRERPVLAKHKADECHRRFHVEGSDFAGILKLWDWQQNLWETVSRTRYRK
                     HCRDHYLSPNRMQEWRDLHNQLRERCSELNMQPNELAANTEAIHRAALAGLLGRMGVW
                     QPEKRSYVGARQTRFVIHPSSALAKKPPAWVFAAEIVETSQPFARVAAILDPAWVESI
                     AGPLCKRTYQDAHWEQRPARVIAKEQVSVLGLPVIRDRRVHYGPIDPKVAREIFLLHA
                     LVRQEFKSKGAFVEHNKRIMDEARRLRDKARKSEMIADDDVLLPFFDNRVPSDVYCGK
                     TFEDWRRQAEKINPRFLFLSLSDVMLGEVADLTPENYPDSISLWGVDFPLRYRFAPGD
                     VDDGITVTLPLSVLARADAEVFEWTIPGWHEEKILLLLQSLPKAFRKALAPMAPLARD
                     IASKRKPFEGPMLRTLSHDVSTIKGVTIPLDAWRPEGLPDHLRFLFRIVDETGRVLGE
                     GRDLKPWQDRLSRPAKELWAKTARCSWERSGLTSWSFGTLPETMPMGAAGGTGYAFPA
                     LVDEVVSVSLRAQPSKSAAIQATRGGLRRLLLIAMGETVERVMRQIPESLPLLTLAAR
                     VADHGLQLREQLVICALDDAFELREPSRYPRDKPAFEACLRTGRPLIPSRIQLVSKVA
                     TEMGTAVAKVESMLENLSGKPGAARSTLDDVRDQLAHLLPKAMLLHVPMERWMHFPRY
                     LKGMQIRLERLVHDPRRDMDKAAKVVPIWQSFHQRKAALLAGGLAERDLDAFRWLIEE
                     LRMCVFAPEIRNPISVSPQKVAEIWKNMGG"
     gene            5234..5563
                     /locus_tag="PK710_14660"
     CDS             5234..5563
                     /locus_tag="PK710_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11017.1"
                     /translation="MLDRRQRRSEKREEALSLFLEAERKRLGVRGLALGTEQGQLIAG
                     AGIGLKRLAAIGASIHTHATSSVLSPSLATWGLRIHDHQFIVTCLGRKFTANLGEGIR
                     RILLACD"
     gene            complement(5601..>7303)
                     /locus_tag="PK710_14665"
     CDS             complement(5601..>7303)
                     /locus_tag="PK710_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11018.1"
                     /translation="TDGERVEGCTNFLWVVMIAALGLVFDDLIVPSRVLGFLLMTVAM
                     GSVVWAARGQRWRDSAGIWMVALAMASMGPIAAWAAGGLEVSLITAGLGVAIALGYEI
                     IAREGRDLRFGLGLGIVLSCLSLTRPDGTLFTASFCLGLVLARGNRRLVWKNAVVAGA
                     MSTASFAALSVFRRLYYGAWVPNTSFKLLVPGSRLEDGWKYISRDHAIWSPLLLLFGL
                     AIVVALCSPHTRRRVVLVIPSLLVWLTYVLFIGGDFMPQRRHWVPALFLFIAIAAEAV
                     RYLVSVRGAALVAWYLTFATPALLLWIQRDDWAVADANNAHWYWTGRPIGLFFQAAFG
                     PKDPLVAVDPAGSLPYFSKLRSLDMLGLNDRFLATHPPELTGKEQVGHELGNGAYVLE
                     RRPDIVVFRLPYGSLTPFFRSGKEMVALPVWQQEYQPFYYRTPTESVEGVAFLRHIGG
                     PLAPRWEDGHLNLPLWLLATSQSARVTLDQGGQMQLILAKGAKAIYRKLSLEGSRCEV
                     EIDATDTVELTVRTDSSAHVARPNAPTSFSTKTPTVSLEIKTTKHEGVLRMVEFACDT
                     "
BASE COUNT         1687 a         2185 c         1932 g         1499 t
ORIGIN      
        1 ctgccgtatg tgcgggctct tgcggttcac tcgtggagtt ggcggtgagc aaaggggtgg
       61 atttgtatct cacgggtgaa atgagccatc ataatgcctt tcgagcggcc agccgaaaca
      121 tgacggttgt gtgcgtcctg cactcccata gcgagcgact gagtttgcag cgtttgaaaa
      181 aaagacttca gcatgcgctg cccaacctcg atgtcatggt gagccaaacg gacaacgacc
      241 ccttcgacat ttgctaatgg atccaggaaa cgaaccgagt atgatggcat gcctatgact
      301 cacgcaggcg gaaacgttcc gatccgagct gtatttggcg gattgaccga cgttggacac
      361 aagcgtgaac acaacgaaga ccacattctc gttcgggtcg acttgggatt gttcatcctt
      421 gcggatggca tgggtggcca taatgcaggg gatgtagcga gcaagcttgc tacctcttcg
      481 ctcgtaagtt ttttcgaagc cacacgaggc caaacgtatt tcggaatacc gccggaagga
      541 tacgatcacc tggatgtcga tggcttacgt ctgctgttcg gaatcatcaa agccaacgag
      601 gatgtctatc gagtctcggc tgccgccggg gcccaccacg gtatgggttc gaccatcgtg
      661 gcgacctaca tcacgagaga cggaaccgtt catatcggcc acgtcggtga tagtcgatgt
      721 taccggatct ccgatgggga gattgaacaa ctcacgcacg accattcgtt cgtcaacgat
      781 gttcttcgcg caaaacccga tatggatccg gagcaattgg ctcatcttcc ccagaacatc
      841 atcacccgcg ctctaggcat gcgcgaagaa gtggagccgt cgattcgatc ggaacccacg
      901 attcccggcg acgtatacct attgtgttcg gatggcttga gcggccaggt cagccgtgag
      961 gagatccatc gcatcgttgg aaccggacaa aatccacagc aagcttgtca aatgctcgtg
     1021 gacgcctcga acgccgctgg tggaaaagac aacgtttcgg tcatcatcat tcgcgtggat
     1081 cccgctcaac cgcgtattcc agctccatct gtggccgtat gccgtcgctg cggttcgccc
     1141 aatactcctg gcgtggcgac ctgtcagcgt tgtgggtcgc cgatgcattg aagcttgctt
     1201 atggcgtccc cggcacgacc ccaagggttc cttccgggtt catcccccca tgtttttcca
     1261 tatttccgct accttctggg gagacacgga gattggattt cgaatttccg gtgcaaaaac
     1321 acacatccta agctcttcga tcagccatcg aaacgcgtcc agatcacgtt ccgccagccc
     1381 gccagccagc aaggcggcct ttcgctggtg aaacgactgc cagataggaa cgactttcgc
     1441 cgctttatcc atgtcgcgac gcgggtcgtg aaccaatcgt tccaggcgta tctgcatccc
     1501 cttcagataa cgtgggaagt gcatccaccg ctccatcggt acgtgcagaa gcattgcttt
     1561 gggcagcaga tgcgccaact gatcacgtac atcatcgagg gtcgaacgtg ccgctcccgg
     1621 ttttcccgac aggttttcga gcatcgactc tactttcgcc accgcggttc ccatttcggt
     1681 cgcgactttg ctaactaatt gaattcgcga tggaattagt ggccggcccg tacgcaaaca
     1741 cgcttcaaac gccggcttat cccttggata gcgagatggt tcgcgcagtt cgaacgcatc
     1801 atccaacgca caaatcacaa gctgttcccg aagctgcagc ccatgatccg ccacgcgcgc
     1861 ggcaagggtc aagagcggca atgactccgg tatctggcgc atcacccttt ccaccgtctc
     1921 gcccatcgct atcaagagca atcgtcgcaa acctccacgg gtggcttgta tggcagcgct
     1981 tttcgaaggt tgcgcgcgca acgacacact gaccacctcg tcgaccaacg cgggaaacgc
     2041 ataacccgtt ccccctgccg cccccatggg catggtttca ggcagcgtgc cgaagctcca
     2101 acttgttaga ccgctccgtt cccacgaaca gcgagcggtt ttggcccaca attccttggc
     2161 cggcctcgac agacgatcct gccatggttt caaatctctt ccttcgccca acacgcgccc
     2221 cgtctcatcg acgatccgga acaagaaccg aagatgatcg ggaagaccct cgggacgcca
     2281 cgcatcaaga gggatggtaa cgcctttgat ggtggaaaca tcgtgcgaca acgtccgcag
     2341 catcggtccc tcgaatggtt ttcgtttcga ggcgatgtca cgggccagcg gtgccatcgg
     2401 agccaacgcc ttgcgaaatg ctttcggcag agactgtaac aaaagcagta ttttttcctc
     2461 atgccatccc ggtatggtcc attcaaacac ttccgcatcc gctcgagcca gtaccgacaa
     2521 cggcaacgtc accgtgatcc catcgtcgac atctcctgga gcaaagcgat aacgaagcgg
     2581 gaagtcgact ccccatagcg atatcgaatc gggatagttc tcgggcgtca gatccgcaac
     2641 ctctcccagc atcacgtccg acagagacaa aaacaagaac cgtggattga ttttctctgc
     2701 ctgtcttcgc caatcttcaa aggtttttcc gcagtacaca tcgctgggaa cgcggttgtc
     2761 gaaaaaaggc aataacacat cgtcatccgc aatcatctcg ctttttcttg ccttgtcccg
     2821 aagccgtcgc gcttcgtcca tgatcctttt gttatgctct acgaacgctc ctttcgactt
     2881 gaattcctga cgaacgaggg cgtgcaacaa gaagatctcc cgagcgacct tgggatcgat
     2941 gggaccatag tgaacgcgtc gatcccggat gacaggaaga cccaatacgg atacttgctc
     3001 cttggcgatg acccgtgcgg gacgctgctc ccagtgggca tcttggtacg ttctcttgca
     3061 caacggaccg gcgatagact ccacccaggc tggatcgaga atggccgcga cacgagcaaa
     3121 gggttgggag gtttccacga tttccgctgc aaatacccag gcgggcggtt tttttgccag
     3181 ggccgaggat ggatggatca cgaagcgcgt ttgccgcgca cccacatacg atcgtttttc
     3241 cggttgccac acgcccatcc gccccagcaa tcccgcgagc gcggctcgat ggatggcctc
     3301 cgtatttgcc gctagctcgt tgggttgcat gttcaattcg gagcaccgct cgcgaagttg
     3361 attgtgcagg tctctccatt cttgcatgcg attgggcgac agatagtgat ctcggcagtg
     3421 ttttcgatac ctagttctcg acacggtctc ccatagattc tgctgccagt cccatagctt
     3481 cagaatcccg gcgaagtccg atccttcgac gtgaaaccga cgatgacact catcagcttt
     3541 atgttttgca agcactgggc gttctcgcgg atcctgaatc gaaagcgacg aggcgacgac
     3601 cagcatttct cgcagacatc cttcgcgttg gccggcgagc agcatcttcg ccaatcgcgg
     3661 atcgataggg aaatgcgaca gttggaaagc aagctcattg ggctccccct gggcatcgag
     3721 cgcgcccagt tcctcgagca cacgataccc atcgcttatc gcccgtttcg aagggggatc
     3781 gagaaaggga aatgcatcga cttgtcccaa acccaacgtc ttcatgtgga gaatgacgcc
     3841 cgcaagcccc acgcgctgga cttcgggcat cgtgaacgcc gggcgagaca tgaaatcctc
     3901 ttcggaataa aggcggtaac agaccccgct gcgcatgcgc cccgcccttc ccgttcgctg
     3961 attcgcgctc gcctgggaga tgcgttccac ttgaagctgc gtcatcccat ttcgaggatt
     4021 gtaccgattg attcgcgcca aaccgctatc caccacatag gcaatccccg gaatcgtcag
     4081 agaggtttcc gccacattgg ttgccaagac gatccgacgt tgccgcaagc tttgaaacac
     4141 tttgtgctgc tcgctttgcg gcatacgacc gtataatgga agcactaccg tatcgggata
     4201 gtcacgagcg atgagaatac tgaaaacctc gtggatttcc cgttctcctg gaagaaatat
     4261 cagcacgtcc cgttgcggtc caagttcggt tatctcgtcg accgcatccg ccaccgcatc
     4321 aaccagatcg caatcgctgg cgggaggatg atagacaact tctactggat acgtccgacc
     4381 tgagatggca aggatcggcg cgtccgcgaa ataagcggca aaccggtcga cctcgagcgt
     4441 ggctgaactg atgatgacac gcaagtccgg tcgttttggg gcaatccgct tcaaacagcc
     4501 tagcagaaaa tcgatgttta gacttcgttc gtgcgcctcg tcgacaatga tcgtgtcata
     4561 accgttcaac catggatcac tccgcaattc cgccaataag atgccgtcgg tgacaaactt
     4621 cacataggtg tctggtgagg tgcgatcgga aaaccggatc ttgaatccca cctcatgccc
     4681 cagttcgaca ccgagttcgt ccgccacacg agatgccaca ctcgttgcgg caatacgacg
     4741 aggctgggta cagccgatcc tggcgcgaag acctcgaccc atggcgaggc aaatcttcgg
     4801 aatctgtgtt gtttttcccg atcccgtctc cccgcagaca atgacgaaag ggtgctgatc
     4861 aatggcgcgg gcaatgtcct gcacccgccc actgatgggc aattcaggcg gaaaatgtac
     4921 cggagactgc gaatgggaag ccacgaaata ctccgtcgta gcatcttttg aacgattcgt
     4981 tagcggtgag atgtcgagat cgtcaacccc tggcaccgtc ccaacgcagg taatggttcg
     5041 accgcgcatc cggacgctaa cattttcatg tgtttcatcc gattaaccga tcatgacggc
     5101 gatcgggatg ctttcttcca tgacccgacg aagcctgccg taccgtaagg cagcgaaaag
     5161 aagaagaagg aagaatcgcg gaggcgatcg tcaaattgtt ggccgtttct gctgatagtc
     5221 tcaggatgcc gccatgttgg atcgtcgtca gcgaaggagc gagaaacgag aagaagcgtt
     5281 gagcctgttt ctcgaggcgg aacgaaagcg cctgggagtt cgtggtctcg cgctcggcac
     5341 ggaacaaggt caactcattg cgggtgcagg tatcgggctg aaacgactcg ccgcgatcgg
     5401 agcctcgatt cacacacacg ccacgtcttc cgtgctttca ccgtccctcg ccacctgggg
     5461 actgcgcatc cacgaccatc aattcatcgt cacgtgcctc ggccgcaaat tcaccgccaa
     5521 tctcggagaa gggattcgtc ggatcctatt ggcctgtgac tgaaagcttc cttggcaaac
     5581 tcgagcctcg aaggattcac tcaagtatca caggcgaatt cgaccatgcg caatacgccc
     5641 tcgtgtttcg tcgtcttgat ttccagggac accgtgggcg ttttggtcga aaacgacgtg
     5701 ggcgcgttcg gtcgtgcaac atgagcgctg gaatcggttc gaaccgtcag ctccacagtg
     5761 tccgtcgcat cgatctcgac ctcgcaccgc gatccctcga gtgacaattt gcgataaatg
     5821 gctttggcac ctttcgccag gatgagttgc atctgtcccc cttgatcaag cgtaactcga
     5881 gccgattgcg aagtggcaag aagccaaagg ggcaggttaa ggtgcccgtc ttcccatcgt
     5941 ggtgcaagcg gaccaccgat atgacgcagg aaggcgacac cttcgacgga ctccgtcggc
     6001 gtacgataat agaagggctg gtattcttgc tgccagaccg gaagagccac catttctttt
     6061 ccggagcgaa agaacggagt caaagagccg taaggcagac ggaaaacaac gatgtcgggt
     6121 cgccgttcga gcacatacgc accgttccct aactcgtggc cgacttgttc cttgccagtc
     6181 agctctggcg gatgcgtagc gagaaatcgg tcgttgagtc cgagcatatc cagggatcga
     6241 agcttggaaa agtacggcag ggagccggcc ggatccacgg cgaccaacgg gtctttgggc
     6301 ccgaaagccg cttgaaaaaa caaaccaatg ggccgtcccg tccaatacca atgcgcattg
     6361 ttggcatcgg caaccgccca atcatcacgc tgaatccaca gcaacagagc aggcgtcgcg
     6421 aacgtcaagt accacgccac cagggccgcc cctctcacgg agacgagata ccgaaccgcc
     6481 tccgcggcga tggcgatgaa caggaacaag gccggaaccc aatgccgacg ttgcggcatg
     6541 aagtctccac cgatgaacag gacatacgta agccacacca acaggctcgg gatcaccaga
     6601 acgacgcgtc gacgcgtgtg cggcgagcac aacgccacca cgattgccaa cccaaacaac
     6661 agcaacaagg gtgaccatat tgcgtggtcg cgggaaatat acttccaccc gtcttccagc
     6721 cgagatcctg gaaccagcag cttgaacgag gtattgggaa cccacgcacc gtaatagagg
     6781 cgtcgaaata cggatagagc agcaaacgat gctgtgctca tcgctcccgc taccacggcg
     6841 tttttccaca cgagtcggcg atttccccgc gccaacacca gtcccaaaca gaacgaagcc
     6901 gtaaacagag tgccatccgg tctcgtcaac gagaggcaag aaagcacaat gccaagtcca
     6961 aggccgaacc gaaggtctcg tccttctcgc gcgatgattt cgtatccaag ggcaatggcc
     7021 acaccaaggc cagccgtgat aagcgatact tcgagccccc ccgcggccca agcggcaatc
     7081 ggtcccatcg acgccatggc cagggccacc atccatatcc ccgcggaatc cctccatcgc
     7141 tgcccccggg ccgcccaaac caccgatccc atggccacgg tcataagcaa aaatccgagg
     7201 acccgtgagg gaacgatcaa atcatcgaac accaaaccga gcgccgcaat catcacgacc
     7261 caaagaaaat tcgtacaccc ctccacacgt tcgccatccg tcc
//
LOCUS       DAOSOR010000279         7296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10223_length_7296_cov_4.294849, whole genome shotgun sequence.
ACCESSION   DAOSOR010000279 DAOSOR010000000
VERSION     DAOSOR010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7296
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10223_length_7296_cov_4.294849"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..544
                     /locus_tag="PK710_14670"
     CDS             74..544
                     /locus_tag="PK710_14670"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOT11019.1"
                     /translation="MRKNQLMLPRPSRHRVRIPCQVVRLRDFKLVADCIENLSLGGIL
                     VGPADPVLTGEPLFLSFRLPCSQHWIDTEATVARVIHGRREGDQTRQLGIKFELLSPS
                     NRDAVRAQLLRMPPIPPCSRPGRRDTASTMNRLSVGSGWSRSSFGHALIRWWDR"
     gene            596..1396
                     /locus_tag="PK710_14675"
     CDS             596..1396
                     /locus_tag="PK710_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11020.1"
                     /translation="MYVVGIAQLAGELQNEVPLLAADLGMAPYDARMLLAPGIPAIVL
                     LSAEKDRAMSLVSKLRERGHDALAFDTRGLVSNGQMVQPRQITISAEGLTSADRYAQH
                     LNFADIFLLVRATHTTTTETSSETTKKSFSLGRAVMTGGLVMRKKTTQTDSAHSYDKI
                     EVLYLYPSTASSPWLLAETGTRYLGLGDRLAPTERANFLKVVDLIRSRAEQARFDDRL
                     VGRKTPTHLLQTTLQTMGDKDRLTTSTNASAVDLLCHLTAMWHFKEAR"
     gene            complement(1440..2159)
                     /locus_tag="PK710_14680"
     CDS             complement(1440..2159)
                     /locus_tag="PK710_14680"
                     /inference="COORDINATES: protein motif:HMM:NF037435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HOT11021.1"
                     /translation="MEPRLDRKGRGPAPDDEACFGTAQVPMLRAAIEDLCWLLSRDYP
                     HEASLKLVGDRYGLTARQRKAVQRSSCSDEARDARVAKRWKGKDLAGADVSIDGFNCI
                     IVTESLLSGAPVFRGRDGALRDMASAHGTWRSLGITNTSVRMLLGVLKQWHAGRVRWW
                     LDQPVSNSGRLRQRLVSLGKEEGIDWEVETVADPDRVLLGCSDVVATADARILDGCSR
                     WVDLPAEVAALTPQVWLVDVS"
     gene            complement(2164..2994)
                     /locus_tag="PK710_14685"
     CDS             complement(2164..2994)
                     /locus_tag="PK710_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11022.1"
                     /translation="MISRPFTNWKLLRGWHDAPQIPLHLEQAARRVLAIPPEETLCVL
                     YDDTLFATGENGFVLTPHRICWKAMGEASVSLLRTSLRTRDVYAKANRVFVGAGELPL
                     TGWNEAMTASLAERLRQWIGQKYLARQAYRVSPPTRQHWTVDEALKRVQPWLTPSKRM
                     HVYPRIASERVKKIRLVFDLASTETVVAVRDTSLLRSGMEGVMLTTACVLGWSAFEKP
                     YKIQWVDLAMESVGARGGVPSGGRCIGSYGKGFVSHGGRDPRIGATMSRTLFRMTVRG
                     "
     gene            complement(2991..3704)
                     /locus_tag="PK710_14690"
     CDS             complement(2991..3704)
                     /locus_tag="PK710_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11023.1"
                     /translation="MEKHIALCLEDLNASHHDERFVQCVALYGREPGLCLDGQGCPLW
                     QTKTDAVCELWRSADGRLVLWKLPNTSSIIVRRASRVLEVPPEKPVILLDKDEIDVGS
                     KTLRVHLHGTTSRMDAPRPLVTRPQRNAFRVAASTVALGATLVASTAGSAQESPRIEV
                     REHPPAPPRMPDPPEPPDAGDHADNTQPANSESGAVLIEDDAQDSAAGDETIEVRETP
                     PAPPPPIRSGGCCSKQPGT"
     gene            3903..>7296
                     /locus_tag="PK710_14695"
     CDS             3903..>7296
                     /locus_tag="PK710_14695"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOT11024.1"
                     /translation="MTRLARFAGFALSIALTASPVIAAPTVNPPDDDSWLSLHVVLQG
                     PCVLDVLDLRQPFGSAGNIDRARRQLDSLRQQHEWMTEQVESLGGEVVASIKRLANVL
                     QVRIPARRASRLTALSGVVRVESVPYYERSLSSAVPFVGAPRAWETPGATGAGIRIGI
                     IDTGIDYLHADFGGSGNPQDYTNNDRTIIEPGTFPTAKVVGGWDFVGDKYNGSSGIKP
                     DPDPLDCFEEESTVIAGGHGTHVAGIAAGMGVNLDGSTFSSSYLASLDPKTFRVFPGV
                     APEASLYALKIFGCDGGTNMVISAIDWAVDPNDDGNFSDHLDVLNMSLGGSYGLQSQT
                     EAEIVKNATLAGMLLVVAAGNDGDTFFVTGEPATYTETLSVAATVDEISYLALRIDSP
                     SSIAGNIPCAEGAFSRPLIETGSISGTLVATEPADACSDLQNASDLSGRIAYIKRGNC
                     YFVDKVQRAVDAGALAAVIVDNVVQNEPFSMGGDGSQKPIPAVLIRNTDGTSLSTHLA
                     GGVNVTLDAHNTYVADIPSDQIASFSSRGPRSIDGRLKPDVAAPGLAILSAGVGSGTD
                     PRETSGTSMACPMAAGAAAVLRQAHPSLSAFDIKAMMMNTTAPMANPKGIPVPVSLGG
                     AGRIRVDDAARQTVTVAAAEPPGAVSLSFGTILTAQTVTTQQDLVISNHGLAPRVFDL
                     SAAPTYPLEGASISVSPSTVTVDPQATATVTVSLTLDPDKLPIEAPDPHTPATLSMGN
                     ESYPRHFITEFGGSIVLSDQENPSLRVPFHAIVRAADRHAAGEAAQCIVGDDPPISIP
                     IQGQGAHKEPVVSVFQHLLSRPLPILPPIPPGEATIVQVGIANDLASVGSFKDGKLYI
                     AVAVAGQWTTPAKGPLSLFSIAIDTTGDFVEDYTVVAEPSSPYRFRDVLRSVTYRQSQ
                     GARVGRADLNAARRDELNTEPYFNSVLIFPVPLAALSELSEDNAAFSFRVTTQQPSFP
                     FLLDRTDWITHDPTKAPVDTARAGYRGMPLYGPGDAVRVYLHREHWDAQGFPSLLLIH
                     HSNELGKRFEVVTLADVGAVYPSDLEVSQNAPVEMNALASSTWPIYVTHQQGSEARDV
                     KLSVSATNGAKIIALSSDSGTCTLSNCTFEKLEDGAQANIEVT"
BASE COUNT         1508 a         2191 c         2029 g         1568 t
ORIGIN      
        1 tcgaaatcgc agtcgcatta ttggacacgt gtgcctacct ttggatacac taagcttcta
       61 atccgaggtc atgatgagaa aaaatcaact gatgttgccc cgtccatccc gccatcgagt
      121 ccgtattcca tgccaggtcg ttcgcctgcg agatttcaaa ttggtggcgg attgcatcga
      181 aaacctgtcc ttgggcggaa ttctggtggg cccggcggac ccggtgctca cgggagaacc
      241 tctatttttg tcgttccggc tcccctgctc ccagcactgg atcgacacgg aagccacggt
      301 ggcaagagtc attcacgggc gtcgggaagg agatcaaacc cgccaattgg gaatcaagtt
      361 cgagttgttg tcgccttcga accgggacgc ggtgcgagca caactgctcc gaatgccacc
      421 cattcccccc tgctctcgtc ctggacgacg tgatacggca tccacgatga accgtctcag
      481 tgtcggttcc ggctggagtc ggtcttcgtt cgggcatgcg ctcattcggt ggtgggaccg
      541 ctgacgatcc ctgtgcctct ttcgttcttg ggcgtcatcc tagaaggaaa cgacgatgta
      601 cgttgtggga atagcgcagt tggctgggga actacaaaac gaagtacctt tgcttgcggc
      661 ggatctcgga atggccccct acgacgctcg aatgctgctt gcaccgggaa ttcctgcgat
      721 cgtgctcctg tcggcggaga aagaccgtgc catgagcctt gtttccaaac tgcgtgaacg
      781 cggccatgat gcgctggcat tcgatacccg agggttggtt tcgaacggtc agatggtgca
      841 gcctcgacag atcacgatat ccgcggaagg cctgacgagt gcggatcggt atgcgcaaca
      901 tctgaacttc gcggatattt ttctgctggt gcgagccacc cacacgacga ccacggaaac
      961 gagtagtgaa accaccaaaa agtctttcag cctcggaaga gcggtgatga cgggcggact
     1021 cgtcatgcgg aaaaaaacga cgcaaacgga ctccgctcat agctacgaca aaatcgaagt
     1081 gttgtacctt tatccttcga cggcaagcag tccgtggttg cttgccgaaa cgggaacacg
     1141 atatctcggg ctcggtgatc gacttgcccc caccgaacga gccaattttc tcaaggtagt
     1201 cgatctgatt cgttcacgcg ccgagcaggc gcgattcgac gatcgactcg tggggcgaaa
     1261 gactcccacg catctattgc agaccacgct gcagacgatg ggcgacaagg ataggctgac
     1321 gacttcgacc aacgcttcag ccgtggatct gctgtgtcat ttgaccgcga tgtggcactt
     1381 caaagaggca agatgaagcc tcgttttctc cgctcgacgg gctcggtgta ttgcctccat
     1441 caagaaacat cgaccagcca cacctgaggc gtaagagccg cgacctccgc cggcaagtcc
     1501 acccatctcg aacaaccatc gagaattctt gcatccgcgg tggccacgac atcggagcaa
     1561 ccgagcaaca cccggtcggg gtccgcgacg gtttccactt cccaatcgat accttcttct
     1621 ttgcccaacg acaccaatcg ttgtcgtaac cggccgctgt tggatacagg ctgatcgagc
     1681 caccagcgca cgcgtcccgc atgccattgt ttcaaaacgc caagcagcat gcgcacactg
     1741 gtgttcgtga taccaagact tcgccacgtg ccgtgagcac ttgccatatc ccgtaacgca
     1801 ccatcgcgcc cacgaaatac gggcgccccg gaaagcaacg attcggtaac gatgatgcaa
     1861 ttgaatccat cgatgctcac gtcggctcct gccagatcct tgcccttcca tcgcttcgcc
     1921 acgcgagcat cacgagcctc atccgagcat gaactacgct gtaccgcctt gcgctgacgc
     1981 gccgtgagcc cataccggtc tcccacgagt ttgagcgaag cttcgtgcgg gtagtcccgt
     2041 gacagaagcc aacacaggtc ctcgatcgcg gcgcgcaaca tcgggacctg ggccgtaccg
     2101 aaacacgcct catcatcggg agcaggtccc ctgcccttgc gatccaacct tggctccacg
     2161 gcatcaaccc ctgacggtca ttcgaaatag ggtacggctc atcgtagcgc caatgcgcgg
     2221 atcgcggccg ccatgggata cgaatccttt cccatacgaa ccgatgcacc ggccacctga
     2281 aggtacgccc ccccttgccc cgacggactc catcgccagg tcaacccatt gaattttata
     2341 cggtttttca aaagccgacc atccgagcac gcacgctgtc gtaagcatca ccccttccat
     2401 tccacttctc aaaagtgacg tatcccgcac agcaaccacc gtctccgtcg acgccagatc
     2461 gaaaaccaat cgaatttttt tcacacgctc cgaagcgatt cgtggataca catgcattcg
     2521 cttggatggt gtcaaccacg gttgcaccct tttcaacgct tcgtccaccg tccagtgttg
     2581 ccgtgtgggg ggtgacactc gatacgcttg ccgcgcaaga tacttttgac cgatccactg
     2641 acggagccgt tcggccaacg atgcggtcat ggcctcgttc cagcctgtca aaggcagttc
     2701 gcccgcgccc acgaacacgc gattggcctt ggcgtacacg tcgcgggttc gcagcgaggt
     2761 gcggagcaaa ctgaccgaag cctcgcccat cgctttccag cagatccggt ggggcgtaag
     2821 cacgaagcca ttttcccccg ttgcgaacaa cgtgtcgtca tacagcacgc ataaggtttc
     2881 ttccggtggg attgcgagaa cgcgccgcgc ggcttgctcg aggtgcagcg ggatttgtgg
     2941 tgcatcatgc catccgcgca aaagcttcca gttcgtgaat ggccgtgaga tcatgtgccg
     3001 ggttgtttgg aacagcatcc gcccgaccgg atgggtggag ggggcgctgg aggtgtttcc
     3061 cggacttcga tggtttcatc acccgctgcg gaatcttgcg catcgtcttc gatgagcaca
     3121 gcgcctgatt cggaattcgc cggctgtgtg ttgtcagcgt gatccccagc atcaggaggt
     3181 tcggggggat caggcatgcg agggggcgcg ggagggtgtt ctcgcacctc gatccgaggc
     3241 gattcttggg cggaaccggc tgtcgaagcc acaagcgtcg cgccgagggc cacggtcgac
     3301 gccgcaactc gaaaagcgtt tcgttgcggt cgtgtgacaa gcggccgtgg agcatccatg
     3361 cgactcgttg ttccatgaag atgaacacga agtgttttgg agccgacgtc gatttcgtcc
     3421 ttgtctagca aaatcacggg tttttcaggg gggacttcga gaacccgcga tgcgcgacga
     3481 acgatgatgg aactggtgtt gggtagtttc cagagcacga gccggccatc agccgagcgc
     3541 cacaattcac aaaccgcgtc cgtttttgtc tgccacaatg ggcaaccttg tccatcgagg
     3601 caaagcccgg gctcgcgccc gtaaagcgct acacattgta cgaagcgttc gtcgtgatgg
     3661 cttgcgttca ggtcttcgag gcatagcgca atgtgttttt ccatgggatt gaagcatacc
     3721 agaatgattg taacaggcgt aggaggcggt cccggtgcct tgggatattt tgaaaattcc
     3781 agtgtttctg aggatatcga agcatccccc cacaacgagt ccaccgttgc ttgaccaact
     3841 tatttccctc gaacggcgct aaccgcattc ccttccttac ccactactcc tagtctgacg
     3901 ccatgactcg tcttgcgcga ttcgccggtt ttgcgctttc catcgcgctc accgcttctc
     3961 ccgttatcgc ggctccgacc gttaaccctc ccgacgatga ttcgtggctc tcgcttcatg
     4021 tcgtattgca agggccgtgt gttctcgatg tgctggattt aaggcaacct tttgggtccg
     4081 cgggaaacat cgatcgagca cgacgacaac tcgactctct tcgccaacaa cacgagtgga
     4141 tgacagagca ggtcgagtcg ttggggggcg aggtagtcgc cagtatcaaa cgacttgcca
     4201 atgtattgca ggttcgcata cccgcgcgac gagcctcgcg gctcacggcg ttatccgggg
     4261 tcgttcgtgt ggaatcggtt ccgtattacg agcgatcctt gagttccgcg gttcctttcg
     4321 tgggggctcc gcgggcgtgg gaaacgccag gagccaccgg agcaggtatt cgaattggta
     4381 tcatcgacac cgggatcgat tatttgcatg cggatttcgg gggaagcggc aacccgcagg
     4441 actacaccaa caacgaccgc acgatcatcg agccgggcac gtttcccacc gccaaagtcg
     4501 tgggtggatg ggatttcgtc ggcgacaaat acaatggcag cagcgggatc aaacctgatc
     4561 ccgatccatt ggactgtttt gaagaagaat cgacggtaat cgctggcggt catggcaccc
     4621 atgtggcggg cattgccgcg ggaatgggcg tgaatctcga tggcagcacg ttttccagct
     4681 cctatctcgc atccctcgac cccaaaacct ttcgggtttt tccaggagtc gcacccgagg
     4741 caagcctgta tgcgctcaag atcttcggct gcgatggggg caccaatatg gtcatttccg
     4801 ccatcgattg ggctgtcgac cccaacgatg acggcaactt cagcgaccat ctcgatgtgt
     4861 tgaatatgtc cctcggaggc tcttacgggc ttcaatccca gacggaagcg gagatcgtaa
     4921 aaaacgcaac cctggccggt atgctgctcg ttgtcgccgc gggcaatgat ggggacacgt
     4981 tcttcgtgac gggcgaaccc gcgacctata ccgaaacgct gtccgttgcc gcaaccgtcg
     5041 atgagatttc gtatctcgcg ttgcgcatcg attccccttc ttccatcgcg ggaaacatac
     5101 cgtgcgccga aggggctttc agtcgtccac taatcgagac gggatccatc tccggaacgc
     5161 tcgtagctac cgaacccgcc gatgcctgca gcgacctgca gaatgcttcg gacctctctg
     5221 gcagaatcgc gtatatcaag cggggaaatt gctatttcgt cgataaggtg cagcgagcgg
     5281 tcgatgcggg tgcccttgcc gcggtcatcg tggacaacgt ggtgcaaaac gaaccttttt
     5341 cgatgggcgg tgacggatcg caaaagccga ttccggccgt attgattcgc aataccgatg
     5401 gcacatcttt gtccactcat cttgcgggag gcgtgaacgt caccctggat gctcacaaca
     5461 cgtacgtggc agatattcct tccgatcaga tcgcgagctt ttcctctcgt ggcccgcgct
     5521 ccatcgatgg ccgtttgaaa cccgatgtgg cggcaccggg gctcgccatc ctttctgctg
     5581 gcgtgggttc aggaaccgac ccgcgcgaaa cgagcggaac gagcatggca tgtcccatgg
     5641 ccgcgggcgc tgcggcggtc cttcgtcagg cccatccatc gctctccgcg ttcgatatca
     5701 aagccatgat gatgaacacc accgcaccga tggctaaccc gaaagggatt cctgttcctg
     5761 tttcgttggg cggtgcggga cggattcggg tcgacgacgc ggctcggcag acggtaacgg
     5821 tggccgcggc ggaaccgccg ggtgccgtat cgctttcttt cggcaccatc ctcacggcac
     5881 agacggtgac cacacagcag gacttggtca tttccaacca cggcctggca ccacgggttt
     5941 tcgatctttc cgcggcacca acctatcccc tcgaaggcgc ttcgatctcg gtgagtccga
     6001 gcacggtaac ggttgatcca caagcaacgg ccacggtgac ggtgagtctc accctcgatc
     6061 cggacaagct tccgatcgaa gcacccgatc cgcatactcc cgccacgttg agcatgggaa
     6121 atgaatcgta tcctcgccat tttattacag aatttggcgg ctcgatcgtg ctctcggacc
     6181 aggaaaaccc aagtcttcgt gtcccctttc atgccatcgt tcgcgcggcg gatcgacatg
     6241 cggccggcga agcagcccaa tgcatcgtgg gcgacgatcc ccccatcagc atccccattc
     6301 aaggacaggg cgcccacaaa gaacctgtcg tctccgtgtt ccaacacctg ttgtctcgtc
     6361 cgttacccat tttgcctccc attccccccg gtgaagccac catcgtgcag gtgggtatcg
     6421 ccaacgatct tgccagcgtt ggctcgttca aggacggaaa attgtacatt gccgtggcag
     6481 tcgcgggaca gtggacgact ccagccaaag gtcccttgag tctgttctcc atcgccatcg
     6541 ataccacggg cgacttcgtg gaagactata cggtcgtcgc ggagccttct tcaccttacc
     6601 ggtttcgcga tgtcctgcga tccgtcacat accggcaaag ccagggtgct cgcgtaggac
     6661 gggcggactt gaatgccgcg cgtcgtgacg aactcaacac ggagccgtac ttcaacagcg
     6721 tattgatatt tccggtgcca ctcgcagcgc tttcggagtt gtcggaagac aacgccgcgt
     6781 tttcttttcg cgtgactacg caacaaccct cgttcccatt cctattggat cgaaccgatt
     6841 ggataaccca cgatcccacg aaagcccctg tggacacggc acgcgcgggc tatcggggta
     6901 tgccgttata cgggccgggc gatgccgttc gtgtttatct ccatcgcgaa cattgggacg
     6961 cacaaggctt cccttccctc ctgttgatcc atcacagcaa tgaattgggg aagcgtttcg
     7021 aggtggtgac tctggcagat gtgggcgccg tgtaccccag cgacctcgaa gtcagtcaaa
     7081 acgcaccggt ggaaatgaac gcattggctt cgtcgacgtg gcccatttac gtcacgcatc
     7141 agcaaggcag cgaagcgcgt gatgtgaaac tatcggtttc agctacgaat ggcgccaaaa
     7201 tcatcgccct gtcttcggat agtggcactt gtacgctttc gaactgcacg ttcgagaaac
     7261 tcgaagacgg tgctcaggca aacatcgagg tcaccg
//
LOCUS       DAOSOR010000280         7287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10243_length_7287_cov_4.577295, whole genome shotgun sequence.
ACCESSION   DAOSOR010000280 DAOSOR010000000
VERSION     DAOSOR010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7287
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10243_length_7287_cov_4.577295"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..1664)
                     /locus_tag="PK710_14700"
     CDS             complement(210..1664)
                     /locus_tag="PK710_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11025.1"
                     /translation="MKRFPWNWACLAGVVVLACGEDDSSTTGAGASGDAASDSALHSD
                     SPVASDSGDVGADSGADSAVDSGSDTAAESGADSGSDTGVDSGSEAGSPDSGTGSTVY
                     PDDPNILSTIDSIGDNTAVELEPYKLGGARVDEWVKNAGNRAPHRRDFCNKIVYAPDR
                     QTGMYAGMNHGVPHKLNDAWEYHLGSNTWYLLYYPEEMLIKPRPAGWYQENIMIEDGY
                     LQTRTHGMPAGSHTWDGLTYEPTIRRLLWANVGEKYFPMETYLAETGQTQAEVEAQLL
                     PGTRLWMYDPPQGRWFKQLGDAPKPTTRTEGASLEYVSHLKKTVWYTAQWNESGMWLY
                     DAGSNTWESLEPNNGVSLYHSGENAFPQAELQMRYSEKHKKLVAVRRKFTYEYDFGTN
                     TWTKVVEDAANEANDARTIFVYDSVNDVFLLLDPKIPSLRSYSITTQAWTTLTPGGAP
                     FPSTYSAGYFDPVHNVMVVYDSKRSVTWLYRYRQ"
     gene            complement(1882..5907)
                     /locus_tag="PK710_14705"
     CDS             complement(1882..5907)
                     /locus_tag="PK710_14705"
                     /inference="COORDINATES: protein motif:HMM:NF005829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HOT11026.1"
                     /translation="MTTVVLAEKPSVARDIAAVLGARRKQYGSLEGDGYVVTWAMGHL
                     VGIAEPHEMNPSWKTWRADLLPLIPQRWDLVLHPETRDHFERVKSLLNAPSVERVVCA
                     TDAGREGELIFRYIYRVARCRKPVLRLWISSLTPEAIRKGFRNLRPSAHFDCLAQAAE
                     ARSRADWLVGMNLSRAYTLRHDDGVLSVGRVQTPTLSMIVDREIAIRDFVPQPYCEVQ
                     ATFNAQTGAYVGTWFDPKRESDRNAPPQRLDGDGVQAETIRARCEGKDGIIESVEGTD
                     KATPPPLLYDLTELQRHANRLFGLRAETTLAVAQSLYEKHKLITYPRTDSRYLPTDVA
                     NSLGPVLDAIAESYGTAVAEGSGTTPLSSRFVQDAKVTDHHAIIPTSNPKKDAQLSRD
                     ELRIYDLICRRLLMAWHQPHETRTTTVVTTVRSTGAVDRFRSSGTMVTKLGWKVLDIA
                     SKKTEQDLLPDGLAVGLRCPVEKVEVLHKETKPPARFNDASLLTAMETAGKLLEDREL
                     EKAMRERGLGTPATRAAIVETLVQRGYVQREGKALVATERGISLVAMVDERIKSPSLT
                     GEWELALKRMEQGDGNLQGFMTGIETLVRDVVQDAKSGKPHRHEPTTTERSPRQALGQ
                     DMAMASETGAAHDQAEEKMASGKETMRANATLGERWKTANSPLSAPTGDLEELLRDRF
                     GFKGFRPFQREVCESTVQGDDGLLVMPTGSGKSLCYQLPGLARGGVTLVISPLIALME
                     DQAGKLSAAGFRAEEVHSGRTREQSRQACRAYLRGEIDFLFIAPERLAVTGFPEMLAR
                     RKPSLIAVDEAHCISHWGHDFRPDYRLLGDRLPLLRPSPVLALTATATIRVQDDIVKQ
                     LGIPTAQRFIRGFRRDNIGIEVLECKPADRAALVRTALSQQDRLPAIVYVPTRKMADQ
                     LGASLGREMRAGTYHAGLETDARARVQEAFASGQLDVIVATVAFGMGIDKANIRTVIH
                     TALPGSIEAYYQEIGRAGRDGKPSRALLLFSWADRHLLDMFLEKSYPPIETIQRLLDA
                     LPRQGLPRESWMQRPRLEPAVVEVAVNKLWIHGAVQIDGDDWVRHARPDWQQDYLDIR
                     AHREAQIEAMFDFARPGTCRMVRLIRHFGDRDDDRACGLCDICQPAACVAARFRRPTG
                     QEVQQMTKMWLELESTRGIAAGTLYRKLFEGSSVQRDRFETWVDALAKAGAIEVIEDS
                     FEKDGKLLRYRKLLKKQQVSFEQVQLPDPAATTEAQVRKGKTRILPSKKPTKTTQPEP
                     PYNPAVAERLRTWRLEQARAKRIPAFRIMTDRTLYAIASQLPSSMETLLLVPGVGPKL
                     ADKYGPKILQLVRRGSSPRSR"
     gene            6122..>7287
                     /locus_tag="PK710_14710"
     CDS             6122..>7287
                     /locus_tag="PK710_14710"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11027.1"
                     /translation="MKASYTARSSARAIHLLAMISLVTAHCGTEQPSSSGVSGTAGSN
                     GGQGGHGVAGDASSTGCEKDTWQCEGTVAWQCDAAGEVQSSLDCAWKGKVCATGLGCV
                     TCHPGSASCSEGIGSSCTEDGTRVETYACDPLQGMRCEPDGCKGACSPSKLGRTHVGC
                     DFWPTITSNTAWSDWFAFGVFVINTTELPAVLTVTRGSEVIATRSLPASGTQTIELPW
                     IRDLKGQDADWEGNVSPSSSSTLSKSNAGAGAYRLRSDQPVVVLQFNTLGAGKDKAAA
                     PGCPIDASSGECLSYSNDASLLLPTSSAGSNHVLMGWRSWPLDSSQGNSRGIGDFASI
                     TALHEGTVVAVTPATRLLSLEPSQGQGFEGGITHEYQLDPGDVLQLISDPSIPNA"
BASE COUNT         1429 a         1978 c         2181 g         1699 t
ORIGIN      
        1 gtccgcccca gggcttgcgt ccctgtcggt gggcgcgtgg aaaggttcgt cctgcccatc
       61 gctgccgcaa tgcaggagcg ccatactcga aacaagcgaa acgacaatac gacgtagacg
      121 catcgtgctt ttccactcca tggcgcgaac gttggaattt tgctactctt gcgtatttcg
      181 agcaccacgc cgaaaccaaa gatgcggttt cattggcgat agcgatacaa ccacgtcaca
      241 ctccgtttgc tgtcgtacac aaccatgaca ttatgaacgg ggtcgaagta cccagccgaa
      301 taggtggagg gaaaaggagc gccccctggc gtaagtgtcg tccaggcctg ggtggtaatg
      361 ctgtaagaac gaagcgacgg gattttggga tcgagcaata gaaatacatc gttgacacta
      421 tcatagacaa atatcgtacg cgcgtcgttc gcctcgttgg ccgcgtcttc caccaccttg
      481 gtccaggtat tggttccaaa gtcgtattca tacgtgaatt tgcgtcgtac cgcgacgagt
      541 ttcttgtgtt tttcgctgta gcgcatctgc aattcagctt gtgggaaagc attctcgccg
      601 ctgtggtaca ggctgacgcc gttgttgggt tcgagcgatt cccacgtatt cgagcccgca
      661 tcatacagcc acattccaga ttcgttccat tgggcggtat accaaaccgt ctttttgagg
      721 tgtgaaacat actcgagcga ggctccctcg gttcgagtgg taggttttgg agcatcgcct
      781 agttgtttga accaacgacc ttgcggggga tcgtacatcc acagtcgggt acctggaagc
      841 aattgcgctt ccacttcggc ctgtgtctga cccgtttcgg ccaaatacgt ttccatgggg
      901 aaatactttt ccccgacatt ggcccaaaga aggcgccgta tcgtgggttc gtaggtcaag
      961 ccatcccagg tatgagaacc ggcaggcatc ccatgggtcc gcgtctgaag atagccatcc
     1021 tcgatcatga tattctcttg ataccagccc gcaggacgag gtttgatgag catttcttcc
     1081 ggataataaa gcaagtacca cgtgttggaa ccgaggtgat actcccaggc gtcattcagc
     1141 ttatgcggca ctccatggtt cattccggca tacattcccg tttgcctgtc cggcgcatat
     1201 acgatcttgt tgcaaaaatc ccgtcggtga ggtgccctgt tgcctgcatt ttttacccat
     1261 tcgtcgacgc gcgccccgcc caatttgtac ggctcgagtt cgacggccgt attatcaccg
     1321 atggaatcaa tcgtactgag aatgttgggg tcatccggat aaacggtact tccggtgccg
     1381 gaatcaggag atcccgcttc agaacccgaa tcgacgccgg tgtcggaacc ggagtccgca
     1441 ccggactcag cggccgtgtc ggaaccggag tcgacggccg agtcagcccc cgagtcagcc
     1501 cccacgtcac ccgaatccga ggccacggga ctgtccgaat gcaaggccga gtctgaagca
     1561 gcatcgccgg aggcgccagc gccggtcgtc gaagaatcgt cttccccgca ggccaaaacg
     1621 acaaccccag ccaagcaggc ccagttccat ggaaaacgtt tcatttccga ttatctcccg
     1681 cacctgcgtg aagcacgatt catgcccgcg gggaattgga cgtaactcat tgaaaactaa
     1741 tattaaaaat atttgcctgc gtcacgattg tcgcgtgcga gactggacgg atcagtcgat
     1801 gtggtccgag gatgtcgcgg gtctagcgcc atgtaggtaa gccgcagcgg cgactggcgt
     1861 cccggcgtgc ggaggcgagg gtcaacggga gcgtggactc gagcctcttc gcaccaattg
     1921 caggattttc ggcccgtact tatcggcgag tttcggcccg acgccgggga cgagcagcag
     1981 cgtttccatc gaagaaggca gttgcgaggc gatggcgtac agcgttcgat cggtcatgat
     2041 tcgaaacgcg ggaatccttt tggctctggc ttgttccagg cgccaggttc gaagacgttc
     2101 cgcgacggcg gggttgtagg ggggctcggg ttgtgtggtt ttcgttggct ttttgctggg
     2161 gagtattcgt gttttccctt tacgtacttg cgcttcggtg gtggccgccg ggtcgggaag
     2221 ctgaacctgt tcaaaagata cctgctgttt tttcaacagc ttacgatacc gaaggagttt
     2281 tccgtctttc tcaaaactat cctcgatgac ttcgatggca ccggctttcg cgagcgcgtc
     2341 gacccatgtt tcaaagcggt cccgttgcac cgaggaccct tcgaaaagct tacgatacaa
     2401 ggtgccggcg gcgatacccc gcgtggactc gagttcgagc cacattttgg tcatctgttg
     2461 aacttcctgg ccggtggggc gccgaaagcg tgcggctacg catgccgcgg gctggcagat
     2521 atcgcaaagc ccgcaagcgc gatcatcgtc gcgatctccg aaatgacgga taagcctgac
     2581 cattctacag gttccagggc gcgcgaagtc gaacatggcc tcgatctgtg cttctcggtg
     2641 ggcgcgtatg tcgaggtagt cctgttgcca atcgggtcgt gcgtgacgca cccaatcatc
     2701 cccatcgatt tgcaccgcgc cgtgaatcca gagtttattg acggcgactt ccacgacggc
     2761 gggttcgagg cgaggccgtt gcatccatga ttctcgtggc aagccttggc gcggtagtgc
     2821 atcgagcaag cgctgtatgg tttcgatggg cgggtacgat ttttcgagga acatgtcgag
     2881 caggtgtcgg tctgcccaag agaacagcag caacgctctc gagggtttgc catcacgtcc
     2941 ggcccggccg atttcttgat aatacgcctc aatggagccg ggtaaggcgg tatggatgac
     3001 ggtacggata ttggctttgt cgattcccat accgaacgcg acggtagcca cgatgacgtc
     3061 gagttgtcct gaggcgaaag cttcttgcac gcgcgcccga gcatcggttt ccaaacccgc
     3121 gtgataggtt ccggcgcgca tttcgcgtcc aaggcttgca ccgagttggt cggccatctt
     3181 gcgtgtgggc acgtagacga tggcagggag acggtcctgt tgcgacaggg ccgtgcgcac
     3241 gagcgcagct cggtccgccg gtttgcattc cagcacttcg atgccgatgt tatcgcgtcg
     3301 aaatccgcgg atgaatcgtt gagccgtggg aataccgagt tgtttgacga tatcgtcttg
     3361 cacccgaatg gtagcggtgg cggtaagcgc gagcacggga cttgggcgaa gaagcgggag
     3421 gcgatctccg aggagtcgat agtcgggtcg gaagtcgtgt ccccaatggg atatgcaatg
     3481 ggcttcatcc acagcgatca gcgacggttt tcgtcgcgcc aacatttcgg gaaaccccgt
     3541 gacggcaagg cgttctgggg caatgaagag aaaatcgatc tcacctcgca aatacgcacg
     3601 acatgcttgt ctggactgtt cgcgcgttct tccggaatgc acttcttcgg cgcggaatcc
     3661 cgcggcgctg agcttgcctg cctgatcctc catcaacgca atcaacgggc tgatgaccaa
     3721 agtcactcca cctcgcgcca atccgggaag ttgatagcac aaggactttc cgctgccggt
     3781 gggcatgacg agcaatccgt catcgccctg caccgtggat tcacaaacct ctcgttgaaa
     3841 ggggcgaaag cctttgaatc cgaatcggtc tcgaagcagc tcctccaagt cccccgtcgg
     3901 tgccgaaagc ggtgagttgg ctgtcttcca tcgttcacca agggttgcgt tggctctcat
     3961 cgtctctttg ccagaggcca ttttctcttc tgcctggtcg tgtgctgcgc ccgtttcgga
     4021 ggccattgcc atgtcttgtc ccaacgcctg ccgaggggat cgctctgtcg ttgtcggctc
     4081 gtggcgatgg ggtttaccgc tttttgcgtc ttgcaccaca tcacgaacga gcgtttcgat
     4141 tccggtcatg aatccttgta ggttgccatc cccctgttcc attcgtttga gagccagttc
     4201 ccactcaccg gtgagagagg ggcttttgat tcgttcgtcg accatggcga caagggagat
     4261 tcctcgttcc gtagcgacga gagctttgcc ttctcgttgg acatatcctc gttgaaccaa
     4321 cgtttcgacg atggctgccc gggtggcggg ggtaccgagt ccccgttccc gcatggcttt
     4381 ttccaattcc cggtcttcca gcaatttgcc tgcggtttcc atcgcggtaa gcagcgaagc
     4441 gtcattgaat cgtgcgggtg gtttggtttc tttgtgcaac acctcgacct tttccacggg
     4501 gcagcgcagt cccacggcca aaccgtcggg aagcaggtct tgctccgttt ttttcgatgc
     4561 gatgtccaag accttccatc ccagtttcgt gaccatggta cccgaagacc ggaaccgatc
     4621 gacggcgccc gtggaccgga ccgtggtcac cacggtggtc gtccgggttt catggggctg
     4681 atgccaggcc atgagcaagc gacgacaaat cagatcatag atgcggagtt cgtcgcggga
     4741 tagttgagca tccttttttg gattcgatgt tggaatgatg gcatggtggt cggttacttt
     4801 ggcatcttgt acgaagcggg aagacagggg cgtagttcct gacccttctg caactgcagt
     4861 tccgtaggat tcagcgatgg cgtccagcac cggtcccagg gagttggcca cgtcggtggg
     4921 aaggtatcga ctatccgttc tcggatatgt gatgagtttg tgtttttcgt agagtgattg
     4981 cgcgacggca agggtagttt cggcacgaag tccgaacagg cgattggcgt gacgttgaag
     5041 ctcggtgaga tcgtataaca ggggtggcgg cgtggccttg tcggtcccct cgacggactc
     5101 gatgattccg tcttttcctt cacaacgcgc gcgaatggtt tctgcttgca cgccatcgcc
     5161 atcgaggcgc tgggggggtg catttcgatc ggattctcgt ttcggatcga accacgttcc
     5221 tacgtacgct cctgtttgag cgttgaaggt agcttgtact tcacaatagg gttgcgggac
     5281 gaaatcacgg attgcgattt ctctatccac aatcatgcta agcgtgggag tttgcacacg
     5341 ccccacggac agcacgccgt cgtcgtggcg aagggtataa gcgcgtgaga ggttcattcc
     5401 cacgagccaa tcggcgcgag aacgggcttc cgccgcctgg gcgaggcaat cgaagtgagc
     5461 gcttgggcgc aggtttcgga agccttttcg gatggcctct ggggtcaggg aagaaatcca
     5521 caatcgaagt actggttttc ggcaccgtgc cacgcgatag atgtaccgaa agatgagttc
     5581 gccttcgcgc cccgcatcgg tagcgcaaac gaccctttcg acggaaggcg cgttcagcag
     5641 gcttttgacg cgttcgaagt ggtcgcgcgt ttccggatga agaaccaaat cccatcgttg
     5701 gggaatcaag ggcaacaggt cagcccgcca cgttttccac gaagggttca tttcgtgggg
     5761 ttcggcgatc cctaccaggt gacccatggc ccacgtcacc acatatccat cgccttcgag
     5821 cgacccgtat tgcttgcggc gcgcgcccaa gacggcggcg atatcgcgag ctacggaagg
     5881 tttttcggca agaacgacgg tcgtcacggt accctcacgg tcctagcgca cgcggcgtgc
     5941 gacgaacagg tttcggacat ggcgctcgtt cgttgcgaac gattcgtgcc ctaccccaac
     6001 ccaagcggac actcaggacc gtggtgattc acgatttcct tctcccctgc actgcccatc
     6061 gcctttgaaa aataatcgtg gcaaaatatg gagttatttg cttttggtcg tgcgccctct
     6121 catgaaagcg tcgtacacag cccggtcttc cgcaagggcc attcatctat tggctatgat
     6181 ttccctggtt acggcccatt gcggaaccga gcagcccagt tcatccggtg tttccgggac
     6241 tgctggatcg aatggtggcc agggaggcca tggggtcgct ggtgatgcct cgtcgacggg
     6301 ttgcgaaaaa gacacatggc aatgcgaagg aaccgtggcg tggcaatgtg atgcggccgg
     6361 agaagtgcag tcgtccctag attgcgcatg gaaggggaaa gtatgtgcga cggggctcgg
     6421 ctgcgtgact tgtcatccgg gttcggcgtc gtgttcggaa ggaattggtt cgtcgtgtac
     6481 ggaggacggg acccgcgtgg aaacgtatgc ttgcgatccg ctgcaaggaa tgcgttgcga
     6541 gcccgacggc tgtaaggggg cctgtagtcc atcgaagctg ggtcggactc atgttggttg
     6601 tgatttttgg ccgacgatca cgtcgaacac cgcatggtct gactggtttg ctttcggggt
     6661 atttgtgatc aacacgacgg agcttcccgc ggtattgacc gtgacgcgag gaagcgaggt
     6721 catcgcgacg cgctcgttgc ctgcatcggg cacgcagacc atcgagttac cttggattcg
     6781 cgatctcaag gggcaggatg cggattggga aggaaacgtg agcccatcgt cttcgagcac
     6841 gttatcgaaa agcaacgcgg gtgcgggagc ttatcgtctt cgttccgatc aacctgtggt
     6901 tgttctgcag ttcaatacgt tgggtgctgg caaggacaaa gcggcggccc caggttgtcc
     6961 gatcgacgca tcgagtggtg aatgtttgtc gtattcgaac gatgcgagtt tgcttcttcc
     7021 tacttcttcc gccggttcga atcatgttct catgggatgg aggtcgtggc cgctggattc
     7081 gagccaaggc aacagccgtg gcattgggga tttcgcatcc atcaccgcgt tgcacgaggg
     7141 aacggtggtt gcggtaactc cggcgacgcg tcttctctcc ctggagccga gccaagggca
     7201 aggattcgaa gggggaatca cgcatgagta tcagcttgat cctggtgatg ttttgcaatt
     7261 gatttccgat ccatcgatac cgaatgc
//
LOCUS       DAOSOR010000281         7266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10285_length_7266_cov_4.516295, whole genome shotgun sequence.
ACCESSION   DAOSOR010000281 DAOSOR010000000
VERSION     DAOSOR010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7266
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10285_length_7266_cov_4.516295"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PK710_14715"
     CDS             complement(<1..357)
                     /locus_tag="PK710_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11028.1"
                     /translation="MTESTVRYGRDVWMALAAVGWADGKLDSEEADAIVRTALDEGIE
                     LEEIADIEEATQNPIDLGDIDLSKLTKEDRLFIFAVANWLARIDGEVTENERAALKKL
                     GETLKIPEKPQLHAEHI"
     gene            complement(501..1334)
                     /locus_tag="PK710_14720"
     CDS             complement(501..1334)
                     /locus_tag="PK710_14720"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HOT11029.1"
                     /translation="MTDVVVCLQCGGIVGAVDDEQACVCPPPSLQAAQLQCPSCGGHL
                     REGARACPYCRATVATLRCPSCSAWNLTSAHHCHTCGKGLDSASSTTQTTSTLACPRC
                     QSHLVNRRYADLEVNECDQCGGLHIEPSMLDRIVQARDASTGLRLALPKREFQKEISV
                     SYVRCPVCERMMNRQVFGRFSGIIVDVCRDHGVWFDAGELAEVLLFVSRGGLERARQR
                     EAEELRETARSARSEALRASMGREITQAGSLDSRGSGKMASLSGMEFIRVLRDIWRGL
                     S"
     gene            1560..2402
                     /gene="infC"
                     /locus_tag="PK710_14725"
     CDS             1560..2402
                     /gene="infC"
                     /locus_tag="PK710_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOT11030.1"
                     /translation="MPRPHFDRRQREPQTRINHRIRADPVRVIGSDGNMLGVLSNREA
                     IRAAQEQGLDLVEVNPKAEPPVCKIMDFGKFKYEEKKRASEAKRKQTTVELKEVKLRP
                     KTDDHDLNFKLRAARRFVEAGNKVKFVVRFRGREIAHPEQAQVQLTWLLEQLEDIANA
                     DSRPAMEGRTMTVLVSPKLQVLQAVSAQKAAEAEKKRIAARKAKSEEDPEGATQEQQT
                     DADQDDLDHESENRSDEPKVPECDSDTFDIFDDDDVVDRSDDDEDLIGEFNLMFDGSK
                     ESDD"
     gene            2589..2873
                     /locus_tag="PK710_14730"
     CDS             2589..2873
                     /locus_tag="PK710_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11031.1"
                     /translation="MADLAHDPLFVRALGVTVGMAGFMLGRVSWQAAAGPRRMTGLLI
                     AIPLLVLALFLVVNPTYAVGWHTTGRTVFIPWIFGVPGVSALLLSRWRVS"
     gene            2858..3673
                     /locus_tag="PK710_14735"
     CDS             2858..3673
                     /locus_tag="PK710_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11032.1"
                     /translation="MARELSQQPSSLTCYPVDIISACHAEPLMTVRKRAFILLFFAIS
                     CGIAAAVLPRLLLNPLATLLASSLGWLAVAPPAFEPALTSTPPSIESASHHCPSQPAP
                     PAGQKPPKNSPARPSGGILVRQDVVRSAVQRGIRPTASPVAATHDHPAGLIVYGWGAA
                     GAGLADGDIITIVGGRQPRSVDDVVIAVAGAYKSPVKAVSGQIWRKGKTLSVTVELPT
                     DNPRSTTRPSASTSSQPLTASPSIRKREVRTFSMPRLVRPSCAQCQRPDNTPP"
     gene            complement(3564..4130)
                     /locus_tag="PK710_14740"
     CDS             complement(3564..4130)
                     /locus_tag="PK710_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11033.1"
                     /translation="MRLIKGHTIALPWVCALGCAGPTAPPSSPATSHVTGIEEFFPLS
                     EGLTLTYQTETHDGVKDMSMVQVHRVHSGLAQMRTGSGVQRLEVTRDSIRKEGGGYFL
                     RMPLQTGASWDGENGGTTVVRSTNMTMTVPAGTFKGCVHTEEEVPEQRARVTVVYCPG
                     VGIARMKVEQDGAWKTFELRAYGSKATP"
     gene            complement(4142..4456)
                     /locus_tag="PK710_14745"
     CDS             complement(4142..4456)
                     /locus_tag="PK710_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11034.1"
                     /translation="MADETDTSSEKETKGDTDRSPFTQDFPDDPDLQLLVAAFEQGRY
                     DIVRARAPKLAQTTDNPEVAKAARELRRRLDADPLSVRLLLATLLLLVVLTSWAYFAH
                     PH"
     gene            complement(4498..4785)
                     /locus_tag="PK710_14750"
     CDS             complement(4498..4785)
                     /locus_tag="PK710_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11035.1"
                     /translation="MRIARYVIVLTVLGCSGADGTAMVRMQAARDFVCAESTVDVHKR
                     VDGSYSAVGCGKSGRYQAICEGVRCSVSKQGEETKTPPSMGLMPESRVSEP"
     gene            4873..5379
                     /locus_tag="PK710_14755"
     CDS             4873..5379
                     /locus_tag="PK710_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11036.1"
                     /translation="MLRLLWSIALAGIVGGALAALIQGLGMVSLSGFVAYPVAAVMGI
                     VIGLVAGKPIWAEGARIEALLKSIFGAILATGLMFALRTWVDFDVSSLGFSETNFGLL
                     PATAFPSIALILSLLFEVDNMFGKGEENKDKKRIATSSATNQRIETADQVEQEEASTQ
                     ANGARRKR"
     gene            5389..6198
                     /locus_tag="PK710_14760"
     CDS             5389..6198
                     /locus_tag="PK710_14760"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOT11037.1"
                     /translation="MSLGRGLLILASVGALALLARSVLIAPIPLWIAMAAFGSYVVIA
                     AAGYFQPRLEMYADVVWRGPSRVAQVALTFEGGPHPRHTREVLKLLESFEAKATFFVY
                     GHKVEAHPEVANEIVARGYGLGIRGYAPDRWLNFRSPNQIMQDLDKALTAIDNATATR
                     PILFRPPHGHIGPRLDTAATRLGLTLVLGSVRASDAFSKVSSKNIIARVQRGLRPGSI
                     IVLHDAVEGDDCRPASLDALAGVLGAIHAQGLRCVDIVQLLEVDEEPSHRR"
     gene            6351..>7266
                     /locus_tag="PK710_14765"
     CDS             6351..>7266
                     /locus_tag="PK710_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11038.1"
                     /translation="MKITNISKRFLSYLLVPGAVLSCSSDPETEGPVVLDAATDSSHD
                     GGMDGSEEIDGSVPDVATDTPSEPTEDSDAPDSALPVTCVSPSHADPAGTDVWNDTLG
                     KATVSIKDRGACHRTYTLSTTAALRHPAEHPNPREIVEMTGWPTLRTGQDMFDALYAL
                     TLQEVRDNSVDVIHDYGFNHGASVPCGSGGCFETGRLWNYVWTRDTAYAVDLGLAAVD
                     PARSLNSLVFKLSERRAGGDAQIVQDTGTGGSYPTSTDRVVWALGASELLRQLDGPSR
                     QAFRDRALEALRNTIEHDRQVVFDSSDGL"
BASE COUNT         1504 a         2151 c         2008 g         1603 t
ORIGIN      
        1 gatatgctcg gcatgaagct gtggtttttc gggaattttt agggtttccc caagcttttt
       61 caaagctgct cgttcgtttt cggtgacctc accatcgatc cgtgcgagcc aattggctac
      121 cgcgaagatg aacaatcgat cctctttggt gagtttgctc aagtcgatat caccgagatc
      181 gatcgggttt tgggtcgctt cttcgatatc cgcaatttct tccagttcga taccctcatc
      241 gagcgccgtt cgaacgatgg catccgcttc ttccgaatcg agcttaccgt cggcccatcc
      301 caccgcagcc aaagccatcc atacgtctcg tccgtatctc accgtagatt ctgtcattgc
      361 accctcccaa gttggaagat gctaaccata gaacacctgc gcactcatcg ccaggggtga
      421 ggagcagcca gctcgattcc accagctttg ctgtggtgat taggattttt atgcggttga
      481 cagaaagatg ggcagaagtc tcacgataga ccgcgccaaa tatcacgcaa cacgcgaatg
      541 aattccattc ctgataacga agccattttc ccggagcctc ggctatcgag ggatcctgct
      601 tgggtaatct ccctgcccat cgatgctcga agcgcttccg aacgagcact cctcgcggtt
      661 tcgcgcagct cttccgcttc acgctggcga gcccgttcca atcctcctcg cgacacgaac
      721 aacaatacct ccgccaattc cccagcgtcg aaccatactc catggtcgcg acagacatcg
      781 acgatgatgc cggaaaaacg gccgaaaacc tgtcggttca tcattcgttc gcaaacgggg
      841 cagcgcacgt aggatacgga gatttccttt tggaattcac gctttggcaa ggcaaggcgc
      901 agtccggtgg acgcatcccg ggcctgaacg atgcgatcga gcatgcttgg ctcgatatgc
      961 aagccaccac attgatcgca ttcgttgact tcgaggtcgg cgtagcgacg attgacgaga
     1021 tggctttggc accgcgggca cgcgagtgtg gaggtggttt gggtggtgga agacgccgaa
     1081 tcgagtcctt tgccgcacgt atgacaatga tgggcggatg tcaggttcca cgcggagcaa
     1141 gagggacaac ggagggtggc cacggtcgcc cgacaatacg ggcaggctct tgctccctcc
     1201 cgcagatggc cgccgcacga cgggcattga agttgggcgg cctgaaggga tggcgggggg
     1261 catacgcatg cttgttcgtc atcgacggct ccgacgattc ccccgcattg cagacataca
     1321 accacgtcag tcatgtcgta catattgcga gcaatcgagg gaaggttcaa tgtcagtcct
     1381 tggaggggcg gtaagcaggc tgttcgaaac cgatcgacag accgggggtt ggtcgcgtac
     1441 acaggggcgt tgcccggggg gcagtcctgg gtccgatggc ccttgccccc atcggcgttt
     1501 acaactcctt tttccttgcc agatcatccg ctttcgggga ggctccgagg gccaataata
     1561 tgccgagacc ccatttcgat cgacgtcaac gagaaccgca gacccgtatc aaccaccgga
     1621 tccgtgccga ccccgtgcgt gtcatcggct cggatggaaa catgcttggc gtgttgtcca
     1681 accgggaagc cattcgcgcg gcgcaagagc aaggattgga cctggtcgag gtcaatccca
     1741 aagccgaacc ccccgtgtgc aaaatcatgg acttcggcaa attcaaatat gaagaaaaaa
     1801 agcgcgcgag tgaagccaaa cgaaaacaaa cgaccgtcga actcaaagaa gtgaaactcc
     1861 gtcccaaaac ggatgatcac gacctaaatt tcaaactccg tgccgcacgg cgcttcgtgg
     1921 aagctggcaa taaggtgaag ttcgtcgtgc gtttccgtgg acgtgaaatc gctcacccgg
     1981 aacaagcaca agtccaactt acgtggttgc tcgaacaact cgaggatatc gcgaatgcgg
     2041 actctcgccc cgccatggaa ggccgcacca tgacggttct cgtttcaccc aagcttcagg
     2101 ttctgcaggc cgtgtctgcg caaaaagccg ccgaagcgga aaaaaagcgt atcgccgctc
     2161 gaaaagccaa gagtgaagag gaccctgagg gcgcgacaca agagcaacag accgacgcag
     2221 accaagacga tctcgaccat gagtcggaga accgcagcga cgagcccaag gtacccgaat
     2281 gtgactctga tacgttcgac atcttcgatg acgatgatgt cgtcgatcgc agtgacgatg
     2341 acgaagacct catcggtgag ttcaacctca tgtttgatgg ttcgaaggaa tccgacgact
     2401 agaatgaagg agagcagctc ctacgacacg ttttctcatt tcgtattgca tcttctcgat
     2461 attccgtgac cacgccactt gctccaggcc cacgacgttt tcgctgggca ccgttgccga
     2521 ccatatctct gttggtcgga agcgtcttgt gtgcgttcgc cctgacttgt tccgtattcc
     2581 caaaactcgt ggctgacctt gcccacgatc ccctcttcgt ccgtgcgctc ggtgtcacgg
     2641 tgggtatggc cggattcatg ctcggacgcg tttcttggca agcagccgcc ggacctcgtc
     2701 gcatgaccgg gttgctcatc gctatcccct tgctcgtgct ggctctattt ctggtagtca
     2761 atcccaccta tgccgtcggg tggcatacca ccggaagaac cgtattcatt ccgtggatat
     2821 ttggcgttcc cggtgtgtcg gcattgttgc tgagccgttg gcgcgtgagc tgagccaaca
     2881 accatcatca ctcacgtgct atcctgtcga tatcattagt gcctgtcatg ccgagccttt
     2941 gatgaccgtg agaaaacgag cgttcatcct tctttttttc gctatttcat gcgggattgc
     3001 cgctgcggtg cttcctaggc tcctgctcaa tccattggcc actctccttg cttcgtcctt
     3061 gggatggctc gccgtcgccc ccccggcttt cgaacctgct ctgaccagca cgccaccgtc
     3121 gatcgaatcc gcttcccacc attgcccgtc acaaccggcc cctcccgctg gacagaaacc
     3181 tccgaaaaac tcgcccgctc gaccatccgg cggaatcctc gtgcgtcaag atgtcgttcg
     3241 ctccgccgtg caacgaggaa tccgacctac cgcttctcct gtcgccgcta cccatgatca
     3301 tcccgcgggg ctcatcgtct atggctgggg tgccgccggt gccggactcg cggatggcga
     3361 catcattacc atcgtaggcg gacgacaacc ccgctccgtc gatgatgtcg ttattgccgt
     3421 cgcaggtgcc tataaaagcc ccgtaaaagc cgtaagcgga cagatctggc gtaagggaaa
     3481 aaccctgagc gtcaccgtcg agttgccaac cgataaccct aggtcaacga cgcgccccag
     3541 cgcatcgact tcatctcagc ctctcacggc gtcgccttcg atccgtaagc gcgaagttcg
     3601 aacgttttcc atgccccgtc ttgttcgacc ttcatgcgcg caatgccaac gcccggacaa
     3661 tacaccaccg tgaccctggc ccgttgctcc gggacttctt cttctgtgtg aacgcaacct
     3721 ttgaacgtgc ctgccggaac ggtcatcgtc atgttcgtgg aacgaacgac tgtcgtacca
     3781 ccgttctccc cgtcccacga ggcacccgtt tgcaaaggca tacgtaggaa atatccgcca
     3841 ccttcttttc gaatgctgtc ccgcgttact tctagtcttt gcacgccaga gcccgttcgc
     3901 atctgcgcaa gaccgctgtg tacgcgatgc acctgcacca tcgacatatc tttgacgccg
     3961 tcgtgtgttt ccgtttgata cgtgagagtc aacccctccg atagggggaa aaactcttca
     4021 attccggtaa catgcgacgt tgcgggagag gaagggggcg cggtgggacc cgcacaacca
     4081 agcgcacaaa cccatggcag cgcgatcgta tgacccttta tcaaacgcat gcccaccacc
     4141 ctcaatgcgg atgggcgaag taagcccatg aggtcaagac gacgagtaga aggagagtag
     4201 cgagtaacaa ccggacggat aggggatcgg catcgaggcg tcgtcgcagt tcgcgggccg
     4261 ctttcgccac ttctggattg tccgtggttt gtgcgagttt cggagcccgc gcccgtacga
     4321 tgtcatatcg tccttgctcg aaggctgcca cgaggagctg aagatccgga tcgtcgggaa
     4381 aatcctgagt gaatggtgag cgatccgtat ccccctttgt ttccttctcg gagctggtgt
     4441 cggtctcatc cgccatagct tcgccttagc acctggcccc ctagaccgcg agggcgatca
     4501 gggctcggaa acccttgatt cgggcatcaa gcccatggat ggtggcgtct ttgtttcttc
     4561 accctgcttg gatacagaac atcgaacccc ttcgcatatg gcttgatacc gtccgctttt
     4621 gccgcatccg acggccgaat acgaaccatc gacccttttg tggacatcga ccgtggattc
     4681 ggcacaaacg aaatctcgag ccgcttgcat tcgaaccatg gccgtcccat ccgcgccaga
     4741 acaccccaaa accgttagaa cgatgacata ccgtgcgatt cgcatgctcg gccctcatgg
     4801 gtaacaacgc caagagccac tgtacaccaa cggagcattg acggcagggg tctgtggagt
     4861 caccctcgca gcatgcttcg actgctgtgg agtatcgcgc ttgcgggaat cgtgggaggg
     4921 gctcttgccg ccctgatcca gggattggga atggtctctc ttagtggttt cgtggcttat
     4981 cccgtcgctg ccgtgatggg tatcgtgatc ggattggttg ccggaaaacc catctgggcg
     5041 gagggagcgc gcatcgaggc tttgctcaag tcgatatttg gagcgatcct tgccacggga
     5101 ctgatgtttg ctcttcgcac ctgggttgat ttcgacgttt cttccctcgg gttcagcgag
     5161 acaaatttcg gtcttcttcc cgcaacggct tttccttcca tcgccctcat cctgtcgctg
     5221 ctgttcgagg tcgataatat gttcggcaag ggcgaggaga ataaggacaa gaaaagaatt
     5281 gctacttcgt cggctacgaa ccagcggatc gaaacggcag accaggtcga acaagaagaa
     5341 gcgtccaccc aggccaacgg cgcacgacga aagcgatgag cgcacacgat gtcgttgggt
     5401 cgtgggttac tcatccttgc tagcgtcgga gccctcgccc tgcttgctcg atccgtgttg
     5461 attgctccta tcccgttatg gatagcaatg gccgcattcg gcagttacgt cgtgattgct
     5521 gcggcggggt atttccagcc aaggctcgaa atgtatgccg acgtggtctg gcgtggccct
     5581 tcccgcgtgg cgcaagtggc tcttacgttc gaaggaggcc cccatccgcg gcatacgcgc
     5641 gaggtgctga aactcctcga atccttcgaa gccaaagcaa cgttttttgt ctatggccac
     5701 aaggtcgaag cgcatccaga ggtggccaac gaaatcgtgg ctcgaggcta tggccttggc
     5761 attcgaggtt acgctcctga ccgatggctg aattttcgaa gcccaaacca gatcatgcaa
     5821 gatctcgata aggcgctcac cgcgatcgac aacgccacag caacccggcc tatccttttt
     5881 cgtccacccc acggtcacat tggccctcgc ctggatacgg ccgcaacacg tttaggcctg
     5941 acgctcgtgc tagggtccgt tcgcgcttcc gacgcattct ctaaggtttc atcaaaaaat
     6001 attatcgctc gcgtgcaacg agggttgcgt ccgggttcca tcattgtctt gcacgacgcg
     6061 gtcgagggcg atgattgccg accggccagt ctcgacgcgc tcgcgggggt ccttggagct
     6121 atccatgcac aagggttgag atgcgtggat atcgtgcaat tgttggaagt tgatgaggag
     6181 ccatcccacc gtcggtagtg cttcgaccgg ggtggccctc cgtgacgcgt cactctatcc
     6241 ttgttgcacg tccctcggcc atcgccgtga ctgggcgttc gtcataggtt gtcttggcgc
     6301 tttgcgcccc gatatgtcac aagagccatc ggatgtgcgg tggagtcccc atgaaaatca
     6361 cgaatatttc caagcgcttt ctttcgtacc tgctggtgcc tggcgcggta ctttcctgtt
     6421 cctcggatcc tgagaccgaa ggacccgtcg tgctggatgc cgcgaccgat tccagtcacg
     6481 atggcggtat ggatggttcg gaggaaatcg atggcagtgt gccggacgtt gcaacggaca
     6541 ccccgtcgga accgaccgag gactcggatg cgccggacag cgctttgcca gtcacgtgcg
     6601 tgagtccctc ccacgcggat cccgcgggaa cggacgtgtg gaatgacacc ttgggcaaag
     6661 ctaccgtttc gatcaaagac cgcggcgcct gccaccgcac ctacacgctt tccacgaccg
     6721 cggcgttgcg tcatccggcc gagcatccca atccaagaga gattgtggaa atgacgggct
     6781 ggcccacgct tcgcaccggt caggatatgt tcgatgcgct gtacgcactg acattacaag
     6841 aagtgcgaga caatagcgtc gacgtcatcc atgactatgg gttcaaccac ggtgcgagtg
     6901 ttccctgtgg aagcggtggt tgtttcgaaa cgggacgttt gtggaattac gtatggacac
     6961 gcgataccgc gtacgccgtc gatctcggac ttgccgccgt cgacccagca cgaagcctca
     7021 actcactcgt attcaagctc agcgaacgtc gagcgggtgg tgatgcgcag atcgtacaag
     7081 acaccggaac gggaggctcg tatcccacaa gcacggatcg cgtcgtgtgg gcgctcgggg
     7141 catcggaact tttgcggcaa ctcgacggcc catcacggca agcatttcga gatcgtgcgc
     7201 tcgaggcgtt gcgcaatacg attgagcatg atcgccaagt cgttttcgat tcgtcggatg
     7261 gtctat
//
LOCUS       DAOSOR010000282         7256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10305_length_7256_cov_3.579086, whole genome shotgun sequence.
ACCESSION   DAOSOR010000282 DAOSOR010000000
VERSION     DAOSOR010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7256
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10305_length_7256_cov_3.579086"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PK710_14770"
     CDS             complement(<1..450)
                     /locus_tag="PK710_14770"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HOT11039.1"
                     /translation="MKYLIGTMLGWMLPVCFVSAAQAADIYVDARNGGKENGTITAPY
                     RTIGAAVKAASSGDRIQIAAGNYAENLHLKKPLTLQGGFVGAQAQTYAAGGSGDFSRS
                     EPSKQESVINGNAEAPVILAEGTIETTLDGLVLRGGKSGVESSAYPED"
     gene            518..2098
                     /locus_tag="PK710_14775"
     CDS             518..2098
                     /locus_tag="PK710_14775"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOT11040.1"
                     /translation="MASQSTIIRKTVGGAAWTVFTSIGSRIIGAVGTIVVTYFISPEI
                     AGEVKVAYILVVLSHELTSFGIGQFVASKPKESPDVVWHATVLHLAIGIPAILLSIPV
                     AYLWSGFFGMPGLMAYIPGLVVAVLFERLHIIPERVLTRELDFRPVSLARGIAEVVYT
                     VLVIGLAMVWTRIGLTPESIGLSMVYANIAKYAFMFTYNAWASKKDVWLTPAKWSWEK
                     ARAIFRFGIPISIAGLSSYASRNLDTLVFAKLFGPAPTGAYDKAYNLADIPAVQVGEQ
                     IGDVLLPSFAQMEEEDRKKALVRSTALLSVIIFPLATGLGATADTLIKTILPQEWWSV
                     GPMLAILSGLAIVRPVGWTIMSYLQACDRPKTVMIIGILKVLVLLAAVALFGKLGQGL
                     GGEAGDEIGPLWACVGVSVGFLFHAISGMWVIKRKDGIPLSKFARACTPPLIACVPMV
                     LAVLGARWLWSRYGIEVRGLAFFVEVIVGAIVYVPAAMLLARETSHDLIHLVKDQIRA
                     RRGEAISVLPPVSSDDLE"
     gene            complement(2437..3879)
                     /locus_tag="PK710_14780"
     CDS             complement(2437..3879)
                     /locus_tag="PK710_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11041.1"
                     /translation="MRRLLFPFSVLLSTLVLHSAAPVHAQQREILDQKLNSAGEGYTV
                     LRVWGSRQQIGYAIGATFADDIVSAFDEVRDMSGNYYSVLRGAMTSLTFLPKGVEEEF
                     NGLVEGVKSVRPEADVDATDMKVLNTFGDWSYACRSHSCWGSRVEAPYSTLSTRRLDF
                     GSPLAVVHHHVLYAIEPSDEPVRWVNLAWPGFVSVVTGVNEYGSLVSVHDFQSSVVAD
                     PGVLSRTMAARHLLSSVPALPIEEHLAWAQQQLSDQPIATGTFINFYAPNGYGGVFTC
                     APGKVCSSPRTPQQNYRDGEVLITTNSQTDGHSTPSGASFLDPYYDTPGPKTLESHFG
                     VMVSDELHMMTVGFRSKGDMRLLAHGRTSEGWTPRIDVEWSTLFPTPTNDAGADAEPP
                     DAPISMDATADSESEIAVESGADSSNSAPPDSSDDPSNSSDEPSEGGCGCKVNGSPST
                     RSWYVFPLGFLALGVFAQRRRGGCAIVDRG"
     gene            4041..5795
                     /locus_tag="PK710_14785"
     CDS             4041..5795
                     /locus_tag="PK710_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11042.1"
                     /translation="MKSKLLSSTLGGLFALMLFVGCSAESDDGSAKPGQTGFSRPPAC
                     YGKCDGPSAGTFLSPYEADLEALHRTYGGAPLKSVEQAFSVQMDLGEVSFTAPTHLFG
                     GPVNIIPYADEDALSDAAGRFFPHRDEIIAKVFPPGSVGFAIKHHRPSHRTISLGGSA
                     ASMKENMKLQDTHIGIVVGIEWNGQPGAITINNPQSYQQGLFGDDDYPMIFVRPVFPG
                     YVSSQLQSAFMDNIRTMMVGFNTVSNFPGDYNGGDPLAANSPEKLQTHVEMMVRAIAG
                     DAAAEQFFRRPENLIYCAELAFVATSAGLLFPLNDTTMIPLVGQQVWDTFQSELHKHN
                     AHERSAFTEQNENELVSSVTLSIAPADLQPIAEYAPANMIEAERQKLAFKPMTMADIV
                     QHFMRTHVPREQMGESLAPVQGQLLGLMRPGLLEAMGMDRLPSSDPRRQAVDALFDNM
                     VQVVSTPYGSYAEFQQAIAPLMDQARAMTGPRDDSGTGLFVPPSLFHVVTQGKHNGGL
                     LQLAYEGHGLHVTQVKLKANPSQPPATPEPVVEPNEPFAGSCVGSCGGVSPDVSCACD
                     DLCVQLGDCCEDYDQHCQ"
     gene            6179..>7256
                     /locus_tag="PK710_14790"
     CDS             6179..>7256
                     /locus_tag="PK710_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11043.1"
                     /translation="MDPKSISQVLETLTQQSWLHGVKAGLAILACVFIFLESQAHTIT
                     EEVRARLLKRLPLVLVGGGLAVIVLQQLADSAKALAVFKNGAIPILGVFAVAIGLFAI
                     GVHGIRFGVMHDRAKQIIGAVLATVAIVFFFLFYHLSYKDYYHRWEFFHYYMGSKYTK
                     ELSYKRIYICAAIADAETGNQTAVKRRKMRDLSATNLLIPSKPFLEEPEICKSHFSEA
                     RWEDFKKDLQFFRRVSGGGYWKDMQKDHGYNPPPIWTIAGHYLSSLQPASSEYFKLLA
                     GLDVTMTALLFLAIGWAFGWRVTIVALLFWGTQEPAPFYWTGGAFLRQDWFFLAIMAA
                     CLARKRYFFLAGFALAYSAGLRIFP"
BASE COUNT         1512 a         1992 c         2094 g         1658 t
ORIGIN      
        1 gtcttccgga taagcgctgg attcgacgcc gctttttcct ccccggagca ccaggccatc
       61 gagggtggtt tcgatggttc cttccgcgag aatcacggga gcctctgcat ttccgttgat
      121 cacagattct tgtttggaag gctcgcttcg ggaaaaatct ccagaaccgc ccgcggcata
      181 cgtttgggct tgtgctccca cgaatccgcc ttgcaaggtc agcggcttct ttaagtgtag
      241 gttttccgca tagttaccag cggcgatctg aatacgatcc cctgaagagg cggctttgac
      301 ggcggcgccg atggtgcggt agggggccgt tatcgtgccg ttttcttttc ccccattgcg
      361 ggcatccaca taaatatcgg ctgcttgtgc cgctgaaacg aagcacaccg gcagcatcca
      421 gcccagcatc gttccgatca ggtatttcat gggctgagcc taacgcgacc gacgaaaggt
      481 cgcaatcacg acaacaagtc ggttatacac gcccatcatg gcttcgcagt ccaccatcat
      541 tcgaaaaacc gtaggaggag ctgcctggac ggtcttcacg agcatcggct cgcgcatcat
      601 cggcgcggtg gggaccatcg tggtcacgta cttcatctcc ccggagatcg ccggtgaagt
      661 gaaagtcgcc tatattctcg ttgtattatc gcacgagctg acttctttcg gcatcggaca
      721 attcgtagcc tccaagccga aagagtcgcc agacgtcgta tggcatgcca cggtgctaca
      781 cctggcgatt ggtatccccg ccatcttgtt atcgatcccg gtagcttacc tctggtcggg
      841 ctttttcggc atgcctggat tgatggcgta tatccccgga ttggtcgtcg ccgtcttgtt
      901 cgaacggctg catatcatcc cggagcgcgt gctcacacgc gaattggatt ttcgccccgt
      961 cagtcttgca cgaggaatag cggaggtcgt ctacacggtg ctcgtcattg ggttggcgat
     1021 ggtatggacg cgcatcgggc tgaccccgga aagcatcggg ttgagcatgg tgtacgcgaa
     1081 catcgcgaag tacgcgttca tgttcaccta caacgcctgg gcttcgaaaa aagacgtctg
     1141 gctgacaccc gccaagtggt cctgggaaaa agcaagagcc atctttcgtt ttggcattcc
     1201 catttccatc gcaggtttgt cgtcgtacgc ctcgcgcaat ctcgacacgc tcgtgtttgc
     1261 caaacttttt ggtcccgccc ccacgggagc ttacgacaag gcgtataacc ttgcggatat
     1321 tcctgccgtt caagtggggg aacagatcgg tgatgtcctt ttgccgtcct ttgcgcagat
     1381 ggaggaagag gatcgaaaaa aggcgctcgt tcggtcgacg gctttgttgt ccgtgatcat
     1441 cttccctctt gccacgggtt tgggggcgac ggctgacacc cttatcaaaa cgattctacc
     1501 ccaggagtgg tggtcggtgg gtccgatgct cgcgattctc tccggtcttg ccatcgttcg
     1561 tccggtgggc tggacgatca tgagttattt gcaagcgtgt gatcgtccta agacggtgat
     1621 gatcatcggt attttgaagg tgttggtgtt gctggccgcg gtggcgttgt tcggcaagct
     1681 cggtcaaggg ctcggaggag aggcgggcga cgagatcggg ccgctgtggg cctgcgtggg
     1741 tgtgagtgtc ggttttcttt tccacgcgat ctccggcatg tgggtgatca aaaggaaaga
     1801 tgggattccg ttatcgaagt tcgcgcgcgc ctgcacgccg ccgttgatcg catgcgtgcc
     1861 gatggtcctc gccgtattgg gagcgcgatg gctatggtcc cggtacggca tcgaggttcg
     1921 tgggctggcc tttttcgtgg aggtaatcgt tggagctatc gtctacgttc ccgctgccat
     1981 gttgctggcc cgagagacat cccacgactt gatccatttg gtcaaagacc agatccgggc
     2041 gcgtcgggga gaggccatca gcgttcttcc gccggtcagc agtgacgatc tggaataggc
     2101 gtgaggtatc gaggatacct gacttcaccg gagtgatggc tcaggtcgca ggcgtaatcg
     2161 cgagcgaccc atgaaaccga agctgacgga gcactgacga aaatcccgaa tcatcccaat
     2221 acggatagga gtgaaagctc ctcccggtcc cctttccccg cattttcccc cggtttttcc
     2281 ttcacggtcc acgatcacga tcacggtcac ggtcacgccc cacgagaaac gatggcggtg
     2341 gtccaaggcg gtcaaggacc gagcccaaat caagaataaa aattctcagc tcgaatccct
     2401 ttccaattca ggaaaaatga agggatctgt ccagcctcat ccccgatcga caattgcgca
     2461 gccgccgcgg cgacgttggg caaatacgcc aagtgcaagg aatcccaaag gaaaaacgta
     2521 ccacgaccgg gtggaagggg agccattgac cttgcatcca catccccctt ccgacggttc
     2581 gtcggatgaa ttggaggggt cgtctgatga atcggggggg gcggagttgc tggaatcggc
     2641 accagattcc accgcgatct cactttccga gtccgcggtt gcgtccatgg aaataggtgc
     2701 gtccgggggc tccgcgtcag cgccggcatc gttggtcggc gttggaaata gcgtcgacca
     2761 ttccacatcg atccggggcg tccagccctc gctcgtgcgg ccatgcgcca acagccgcat
     2821 atcgcctttc gaacgaaatc cgaccgtcat catatgcagt tcatcactca ccataacacc
     2881 gaagtggctt tcgagggtct tgggcccggg ggtgtcgtaa tacgggtcca aaaaactggc
     2941 tcccgacggc gtgctatgac cgtccgtttg actgttggtg gtgatgagca cctccccatc
     3001 ccgatagttt tgttgagggg ttcgtggaga cgaacaaacc ttgccaggag cgcaggtgaa
     3061 aacgccaccg tagccgttgg gcgcatagaa gttgatgaac gttcctgtgg caatgggctg
     3121 gtcggaaagc tgttgctggg cccaggcaag gtgctcctcg atgggaagag caggaacgga
     3181 ggaaagcagg tggcgggctg ccatggttcg ggaaagcact cccgggtcgg caacgaccga
     3241 ggattgaaaa tcgtgtaccg aaaccagcga accgtattca ttgacgccag tcaccacgct
     3301 gacgaatccc ggccatgcca gattgaccca ccgaaccggc tcgtcggaag gctcgatagc
     3361 gtaaagcacg tgatggtgga cgacagcaag aggcgaaccg aagtccaggc gtcgcgtgga
     3421 tagagtcgaa taaggagctt cgacgcgtga tccccaacag ctatgggacc ggcaggcata
     3481 cgaccaatct ccgaacgtat tgagcacttt catgtcggtg gcgtccacgt cggcttctgg
     3541 ccgcacggat ttgacacctt cgaccaagcc attgaactcc tcctccacgc ctttcggcaa
     3601 aaaggtcagg gaagtcatgg ccccgcgcag gacagaatag tagtttccgg acatgtctcg
     3661 cacttcatca aaggcagaaa cgatgtcatc ggcgaaggtg gctccgatgg catagccgat
     3721 ttgctgacgc gatccccaga ctcggagcac cgtgtagcct tctcccgccg aattcagttt
     3781 ttgatcaagg atttcacgct gttgcgcgtg aaccggtgcg gcggaatgaa gtacgagtgt
     3841 cgataagaga acggaaaacg gaaaaagaag gcgtcgcatg aagatcgttt tccacggtct
     3901 gcatggcgag tcaaggcggg cacctcggga ttctttttcc atctttgggc cgggcatgaa
     3961 aaatggtact ggggctttgc cgcttcctca tgatatggag cattttggaa ataactgggg
     4021 cttatcgaga gggtgccatc atgaaatcaa aacttctttc atcgactctt gggggattgt
     4081 tcgcgctgat gcttttcgtc gggtgctcgg cggagtctga cgatggctcg gcgaagcctg
     4141 ggcagactgg gttttccagg ccgccggcgt gttacggcaa gtgcgacggc ccctctgctg
     4201 gcacgtttct gagtccttat gaagcggatc tcgaggcgct acaccgcaca tacgggggtg
     4261 ctcccctgaa aagcgtagaa caggctttca gcgtccaaat ggaccttggg gaggtatcct
     4321 tcacggcacc gacgcatcta tttggcggtc ctgtcaacat cattccttac gcggacgaag
     4381 acgctttgtc ggacgccgcg gggcgatttt tccctcatcg cgacgagatc atcgccaagg
     4441 tatttcctcc cggaagtgtc ggctttgcca tcaaacacca tcgcccttct catcgcacca
     4501 tatcgctagg gggaagcgca gcgagcatga aagaaaacat gaagctgcaa gatacccata
     4561 tcgggattgt ggttgggata gaatggaacg gtcaacctgg ggctatcacg atcaacaatc
     4621 cacaaagcta tcagcagggg ctatttggcg acgatgatta tccaatgatt ttcgtccgcc
     4681 ccgtgttccc cggctatgta agtagccaat tacagagcgc ttttatggat aacatccgga
     4741 cgatgatggt gggtttcaac acggtctcga attttccggg ggactacaac ggcggcgatc
     4801 cgttggcggc caacagtccc gaaaagctgc aaactcatgt ggaaatgatg gttcgcgcca
     4861 ttgctggcga tgccgcggcc gaacagttct tccgtcgccc ggagaatctc atttattgcg
     4921 ccgaactggc ctttgtggcg acgagcgcgg gattgctgtt ccctctcaac gacacgacca
     4981 tgatcccgtt ggtagggcag caagtatggg acacgttcca atccgaactc cacaagcata
     5041 atgctcacga acgttccgcc tttacggagc agaacgaaaa cgaacttgtt tcatcggtca
     5101 cgttgtcgat agccccggcg gatcttcagc ccatcgccga atacgcccct gccaacatga
     5161 tcgaagcgga gcgccaaaaa ctcgctttca agcccatgac catggcggat atcgttcagc
     5221 atttcatgcg aacccacgtt cctcgtgagc aaatgggaga atcgcttgct cccgtgcagg
     5281 gacagctcct cggcctgatg cgtcccggct tattggaagc gatgggtatg gatcgccttc
     5341 cctcctcgga ccctcgtcgc caggctgtcg atgcgctttt cgacaatatg gtccaagtcg
     5401 tttctactcc ttatggaagc tacgccgaat tccagcaagc cattgctccg ctcatggatc
     5461 aagcgcgcgc gatgaccggg cctcgcgacg attcaggaac ggggttattc gttcctccaa
     5521 gcctatttca tgtcgttacc caagggaaac acaacggtgg actgctgcaa ctcgcctacg
     5581 aaggtcacgg gctgcacgtc acgcaggtca agctcaaagc caatccttcg caacctccgg
     5641 ccaccccgga acctgtcgtg gaacccaatg aaccgttcgc tggttcatgt gtcggttctt
     5701 gtggtggcgt ctctcccgac gtaagctgcg cttgcgacga cctctgcgtt caactgggtg
     5761 attgttgtga ggattatgac cagcactgcc agtgattttg ccgactagcc gatacgccag
     5821 ccaattatct caccgaaaat cctcctacgt atttgccgcc tgaaaacgcg ggtgaatcat
     5881 gggagccgct gcctggccct cgtccagata accgaatcgg gatagacgtc gactacggcc
     5941 attcgccaag gatatgattt catgccgggt ggagtgggac gcggggtgga ggggtgctaa
     6001 ccaccctttt gcccttacct cttggaatgc cgtgcggtga gggtaagaaa cgcgaaccca
     6061 gtagggtcga ttgtagcgag aacccgcgga ttgcgcaaag ggggtcgagt gtcggggcgg
     6121 gaaggtaaaa aaagcgctct cccccacgac gaagaaccgc gctatggtgg gcgcgctgat
     6181 ggatcccaag tcgatttctc aagttctaga aacgctcacg cagcagtcat ggcttcacgg
     6241 agtgaaggcg ggtttggcga tattggcctg cgtgttcatc ttcctcgaat cccaagcgca
     6301 tacgattacg gaagaggtcc gagcgcggct gctgaaacgc ttacctttgg tgctggtcgg
     6361 cggtggcctt gcggtgatcg ttcttcagca gctcgcggat agtgcgaaag cgctggcggt
     6421 tttcaaaaac ggggcgattc cgattctcgg cgttttcgcg gtcgcgatcg gtcttttcgc
     6481 catcggcgtc cacggaattc gatttggagt gatgcacgat cgtgccaaac agatcatcgg
     6541 agcggtgctt gccacggtcg ccatcgtttt cttcttcttg ttttatcacc tgtcatacaa
     6601 ggactattac caccggtggg agttcttcca ttactacatg ggctccaagt acacgaagga
     6661 gcttagctac aagcgcatct acatttgcgc cgcgatcgcc gacgccgaaa ccggcaacca
     6721 aaccgcggtc aaacgacgca aaatgcggga tctttccgcc accaacctgt tgattccctc
     6781 gaagcctttt ctcgaagagc cggagatctg caagtcgcac ttttccgagg cgcgatggga
     6841 ggacttcaaa aaggacctgc agtttttccg tcgtgtttcc gggggcggat actggaagga
     6901 catgcagaag gatcatggct ataacccgcc gccgatctgg actatcgccg gacattattt
     6961 gtcatcgctg cagccagcgt cctcggagta tttcaagtta ttggcgggcc ttgacgtaac
     7021 gatgaccgcc ttattatttc tggcgattgg ctgggctttt ggttggcgcg taacgattgt
     7081 ggcgctctta ttttggggca cgcaggaacc tgcgccattt tattggacgg ggggagcgtt
     7141 ccttcgtcag gactggtttt tccttgcgat catggcggcg tgtttggctc gaaaaagata
     7201 ctttttcctt gccggattcg ctcttgccta ctccgcggga ttacgaatat ttccga
//
LOCUS       DAOSOR010000283         7253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10311_length_7253_cov_4.288552, whole genome shotgun sequence.
ACCESSION   DAOSOR010000283 DAOSOR010000000
VERSION     DAOSOR010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10311_length_7253_cov_4.288552"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /locus_tag="PK710_14795"
     CDS             <1..1296
                     /locus_tag="PK710_14795"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOT11044.1"
                     /translation="ACEALVVESKRPLRVAVVGEFNAGKSSFINAVVGADVAPTGVLP
                     TTASLHWLSWAPDPYARIVTREDTDRIVTHEGLKGALAALQKSDTPIREVHICAPIER
                     LYRIEILDTPGFNAPDMDHVAAATRAVHEAHVAIWVLDATQALKDSERKVLARIADVG
                     TPVQILINKLDRLTPEKLQQVMGHVQTGLASIGLESMAGIVAFSARLALAGRLGDVQS
                     MQASRWQDVETLLSQQIENRSGTLRARALRRKASRIATELSELMQQQDAAPKSHREAR
                     EWEASASRLLSLERTQYERIVQRLEPATARVREDLRPIRVAGMSMDDINASAYAEARL
                     LVRMTQPMASAIAEVAEVHQRFEPEIRDAVGLVLRGTFASAKSIDDFLQFPSWRVVRA
                     CALASHERIVVAGERAAGAVAAHPRWMRLQALRESLTRD"
     gene            1800..2321
                     /locus_tag="PK710_14800"
     CDS             1800..2321
                     /locus_tag="PK710_14800"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HOT11045.1"
                     /translation="MKHRVWWIGGAVGLISTGWLMVPSVHAESIAEVDGQPVTVEADR
                     VEVDLKSGKAMLAGAVRVRRGELEVTCKALEASYDKAPSIRWAKATGDVRARMRDWEM
                     RSEQAEYEMGRRMVQARGDVQVRRAGAWVRASEVSFDWATERMTMHKVRGSIPLATVM
                     PLIPSSSGVSTAR"
     gene            2318..3031
                     /locus_tag="PK710_14805"
     CDS             2318..3031
                     /locus_tag="PK710_14805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT11046.1"
                     /translation="MSVVLEAQGVEEYRSGKAVLRGVSIRLHSGRVAALLGPTGAGKT
                     TLFDVLVGERRPQRGVVRLMGEDVTKLPMWRRARLGVGTIPQGPTVLPDMDVDGNLTT
                     FERLGGGERKGSLYWARMVGLEQRLGVRAGHLSGGERRLLEIARSMVTRPRVLICDEP
                     FSGIDPAGAMRIAQMLRGLALEGVAVLLSDHHASIALRISDEVGLLLDGEVHFWGSAS
                     DWIKEPLVRERYLGGSMDG"
     gene            3153..4679
                     /gene="rpoN"
                     /locus_tag="PK710_14810"
     CDS             3153..4679
                     /gene="rpoN"
                     /locus_tag="PK710_14810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOT11047.1"
                     /translation="MEIKHQLTQKQTLSVVMTPQLQQAIRLLQLSRIELIEEVRREMD
                     ANPMLSDEDIEPRHREVTDAELPKSVPGEAPPEERVGETERHEANTDLRGREEERRAK
                     EVDWDQFLENRTLQQPVPGEGRSGFEELPPIEQNLTKPRSLADHLRWQLQVSDFVDEE
                     RRFAEFVIGNLDENGYLDLKGVEHTDGTKTRDWTVEDLAAEAGLHPDDAEEVLKMIQL
                     LDPVGVASRDLRECLLVQAEHYGYSDLECEMISQHLHNLEKRAYQAIAKDLKIPIEEV
                     YEAAKEIQKLESRPARNFTDTDDRTIGITPDVYVIKDGDDFVVLDNDRGLQRLYVNDA
                     LAKKLRQDPAAKDYIDEKLRNAQWLIRAIEQRRKTIIRVTECIVKMQREFFENGVAYL
                     KPMILKDVAEEVSMHESTISRVTTNKYVHTPQGLFELKYFFNSSIRRVADEDIASESV
                     KQWIKKIIDEENKEKPLSDQTIVKMLKDKEGIQIARRTVAKYREMLGILASSKRKQLF
                     "
     gene            4753..5091
                     /gene="raiA"
                     /locus_tag="PK710_14815"
     CDS             4753..5091
                     /gene="raiA"
                     /locus_tag="PK710_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOT11048.1"
                     /translation="MNIAITFRQLEASEAVKQYATDKVAKLQKFLRKPLSAQVTLSLD
                     KLDHVADIHVHAGSDHFHGSERSEDMYASIDKVIDKLDRQIRGSKGVLQTKKRGSEGL
                     KAREVIEPKD"
     gene            5258..5728
                     /locus_tag="PK710_14820"
     CDS             5258..5728
                     /locus_tag="PK710_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOT11049.1"
                     /translation="MRLTDILTEDRVRVTRHQDGIKVKSEVLEALSLLLASGTGQAAE
                     LIHKTLTDRESLQSTGIGDGVAIPHGSLEALVTQTAAIVLCPDGVDFHSIDGRPAKIF
                     VGVIGPRGSTGEHLRMLARISRLLRDGNLRDRLLEASDGTQAFDVIVEEERGRP"
     gene            5758..6684
                     /gene="hprK"
                     /locus_tag="PK710_14825"
     CDS             5758..6684
                     /gene="hprK"
                     /locus_tag="PK710_14825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOT11050.1"
                     /translation="MRELFADAKLGLSIVEMVGRQGDSAQVIHNRIQRVGLALAGHFH
                     GVVPSRVQVCGETEISYLKTMTSEQRSAALGAFLALQVCSVVVTGGLPLPEEMIEQAE
                     RNRCLVVRISERTGHVIQAIEGYLDERLAPRMQLHGVLVDVFGVGMLLLGKSGIGKSE
                     CALDLVLRGHRLVADDWVLCDFRHPGHVFGAPASLLRHLIEVRGLGILNVKTLFGVTA
                     VRDRKRIDIIVRLCSATDEPEQERLGIEDRFHELLGVKIREIALPVRAGRDMAAIVEI
                     AARDELSRRAGHHSAKEFYDTIERRLQHEGNS"
     gene            6681..>7253
                     /locus_tag="PK710_14830"
     CDS             6681..>7253
                     /locus_tag="PK710_14830"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOT11051.1"
                     /translation="MTNDSSRASDSNGQLADPKIFERIVIVSGMSGAGKSTALHALED
                     LGYFCIDTLPTTVIEPVLEACRNASVKWIALGIDVRVRMFLEHFSSALDLIQSTPNVD
                     LSVLFLDASDQTLLSRFSATRRPHPLSTNVQGPDCGAMALLDGITSERQRLARVRVRA
                     TDVIDTTGMTVHDLRRRVVELLGGETSRGRM"
BASE COUNT         1584 a         1682 c         2438 g         1549 t
ORIGIN      
        1 gcttgtgaag ccttggtggt ggaatcgaaa aggccgttgc gtgttgcggt tgtgggagag
       61 ttcaacgctg gcaagagttc gttcatcaat gcggtggtgg gagcggatgt ggcgccgacg
      121 ggtgttttac cgacgacagc ctctttgcat tggctgagtt gggcaccgga tccttacgcg
      181 cgcattgtaa cgcgggagga tacggatcga atcgtcacgc acgagggatt gaaaggggct
      241 ctggcggctc ttcaaaagtc cgatacgccg attcgggagg tgcatatttg tgcgccgatc
      301 gagcgtcttt atcgtatcga gatccttgac actcccgggt tcaatgcgcc ggatatggat
      361 catgtggcgg ccgctacgcg agcggtgcat gaggcgcacg tggccatttg ggtgctcgat
      421 gctacgcaag cattgaaaga cagtgaaaga aaagtgcttg cgcgcattgc cgacgtggga
      481 acgcccgttc agattttgat caacaagctc gatcgtttga cgccggaaaa actgcagcag
      541 gtgatgggcc atgtgcagac ggggctggca tcgatcggtt tggaatccat ggcggggatt
      601 gtcgcgtttt cggcgcgact ggcgctggca ggtcgattgg gtgacgttca gtccatgcaa
      661 gcatcgcggt ggcaagatgt agaaaccttg ttgtcgcagc aaatcgagaa ccgtagcgga
      721 accttacgag cccgcgcctt gcgtcgaaag gccagtcgca ttgcgacaga gctgagcgaa
      781 ctcatgcaac agcaagatgc ggcaccgaaa agccataggg aagcaaggga gtgggaggcg
      841 agcgcctcgc gtttgttgag ccttgaacga acgcaatacg agcgaatcgt acagcggttg
      901 gagccagcga ccgcgcgagt tcgcgaggac ctgcgtccga ttcgtgtcgc gggcatgtcc
      961 atggacgaca tcaatgcgtc ggcgtatgca gaagcgcgtt tgctggtgcg catgacgcaa
     1021 ccgatggcta gcgccatcgc ggaggttgcg gaagtacatc aacggtttga gccggagata
     1081 cgcgatgcgg tggggttggt attgcgcgga acgtttgcgt cggcaaaaag cattgatgac
     1141 ttcctgcaat tcccttcctg gcgcgtggtt cgggcgtgtg cgctggcgtc gcatgaacgg
     1201 atcgtcgtgg ctggtgagcg cgcggcaggg gcggtggcgg ctcatccgcg atggatgcga
     1261 ttgcaagcgt tgcgggagtc tttgacgcgg gactgaagcg atagacgaat caaacgtacc
     1321 catgggctgt ttcgtggtgc tttcacaccc caacgccaac aacaccatga gggctcctac
     1381 atgcggtccc atgagccata gtacgggatg ggccgcggca gcttccttgc tctcttctcc
     1441 cccgggtgga taagaaagcg tagggcaggt ggaacggcta tagccgtcaa cggcagcgcg
     1501 cgtgggaata cgagccaaag gtgttgcatg tgcaatcaac ttcgaagcgg ggaagggcag
     1561 atggatcgaa tttgctcatg aggatggttt aggaatggta gtatccgaat acggatactt
     1621 cggacgcacg aggagggatg ttcggatcga gaggccgaag gggtgagcat tcgttttggt
     1681 gagtgtcgaa cgccgggcgg acaagccgaa tcaaaaggga agcatgggtc gatggcaatg
     1741 gacggttctt cggagaaaca tgggttgcgc ggggcgtggg attacggcac ggtaggcgcg
     1801 tgaagcatcg agtgtggtgg ataggcggcg cggtcgggct gatatcgacg ggatggttga
     1861 tggttccgtc ggtgcatgcc gagtccattg cggaggtaga tggacaaccg gtgaccgtgg
     1921 aggctgaccg ggtcgaggtg gatttgaaaa gcggcaaggc gatgcttgcg ggggctgttc
     1981 gcgtgcgacg gggggaattg gaggtcacgt gcaaggcgtt ggaggcgtcc tatgacaagg
     2041 cgccttcgat tcgatgggcg aaagcaacgg gtgatgtgcg tgcgcggatg cgagactggg
     2101 aaatgcggtc cgagcaagcg gagtacgaga tgggtcgtcg catggtgcaa gcgcgagggg
     2161 atgtgcaggt acgtcgagct ggggcgtggg ttcgggcgag tgaagtatcg tttgattggg
     2221 cgacggagcg gatgacgatg cacaaggttc gcggttcgat accgctggcg acggtgatgc
     2281 cgttgatacc ttcttcgtct ggggtgagca cggcgcgatg agcgtggtgt tggaggcgca
     2341 gggggtagag gaatatcgaa gtggcaaggc tgtgttgaga ggggtgagca ttcggctaca
     2401 ttcggggcgc gtagcggcgt tgctagggcc gacgggggcg gggaaaacga ctttatttga
     2461 tgtattggtg ggcgagaggc ggcctcagcg cggggtggtt cgattgatgg gcgaggatgt
     2521 cacgaagctt ccgatgtggc gtcgcgcgcg gttgggagtg gggacgattc ctcagggtcc
     2581 gacggtattg ccggatatgg atgtggatgg caatctgacg accttcgagc ggcttggggg
     2641 tggcgagcga aagggatcgt tgtattgggc gcggatggtg ggtttggagc agcggctagg
     2701 ggttcgtgcg ggccatttgt ctggggggga gcgtcgattg ttggagatag cgcgcagcat
     2761 ggtgacgagg ccgcgggtat tgatttgtga cgaaccgttt tcggggatcg atccggctgg
     2821 ggcgatgcga attgcgcaaa tgctgcgagg acttgcattg gagggggttg cggtattgct
     2881 atccgatcac catgcatcga tagcattgcg gataagtgat gaagtgggct tgttattgga
     2941 tggagaggta catttttggg gatcggcatc ggattggata aaggagccac tcgttcggga
     3001 gcgatacttg ggggggtcga tggatgggtg aggtgcaatt tggtcgcgac cttacgagcg
     3061 gctggtgcta ccatagtgag cgttcatgcg atgcatggcg taaatgcggg taactcgagg
     3121 aacacggctc gggatcggca ctttgtattc cgatggagat aaaacatcag ctaacgcaaa
     3181 agcaaacgct ctctgtggtc atgactccgc agttgcagca ggccattcgg cttctgcaac
     3241 tcagtcggat tgagttgatc gaagaagtgc gcagggagat ggacgccaac ccgatgttat
     3301 cggacgagga tatcgagcct cggcatcggg aagtgaccga tgcggaatta ccgaaatcgg
     3361 tgccggggga agctccgccc gaggagcgag tgggagagac ggagcgtcac gaagccaata
     3421 cggatttgcg ggggcgcgaa gaagagcgca gggccaagga ggtcgattgg gaccaattcc
     3481 ttgaaaaccg cacgttgcag cagccagtac cgggggaagg gcgaagtggg ttcgaggaat
     3541 tgcctccgat cgagcagaat ctgacgaagc ctcgcagttt ggcggaccat ttgcgatggc
     3601 agcttcaggt cagtgatttc gtggatgagg agaggcgttt tgcggagttt gtgatcggca
     3661 atttggatga gaacgggtat ctcgatctga agggggtaga gcacacggat ggcaccaaaa
     3721 cacgggattg gacggtggag gaccttgcgg cggaagcggg gctccatccc gatgatgccg
     3781 aagaggtgct aaaaatgatc cagcttctcg atccggtggg ggtggcgtcg cgagatttgc
     3841 gggagtgttt gctcgtgcag gccgagcatt atgggtattc ggatttggaa tgcgagatga
     3901 tcagccagca tctgcacaat cttgaaaaac gcgcctacca agcgatcgcg aaggatctaa
     3961 aaatcccgat tgaggaagtc tatgaggccg ccaaggagat tcaaaaactc gagagtcgtc
     4021 cagcgcgcaa cttcacggac accgatgacc gaaccatcgg catcacgccg gacgtctacg
     4081 tgatcaaaga cggcgatgac tttgtcgttc tcgacaacga tcgcggtctg cagcgtttgt
     4141 acgtcaatga tgcccttgcc aaaaagctaa ggcaggatcc tgctgcgaag gactatatcg
     4201 acgaaaagct tcgcaatgcg caatggctca ttcgcgcaat cgagcagcgt cgcaaaacga
     4261 tcattcgggt caccgaatgc attgtgaaga tgcagcgaga gttttttgaa aacggtgtgg
     4321 cgtacctcaa accgatgatt ctcaaggacg tcgccgagga agtgtcgatg cacgagtcga
     4381 ccatttcgcg tgtgacgacg aacaagtacg tgcatacacc ccaggggctg ttcgagttga
     4441 agtacttttt caactcgtcc attcgtaggg tagcggatga ggacattgcc agtgagagcg
     4501 tgaaacaatg gataaaaaag atcatcgacg aagaaaacaa ggagaaaccg ttgagtgatc
     4561 agaccattgt gaaaatgctg aaggacaagg aggggataca gatcgcgcgc agaacagtgg
     4621 caaagtacag ggagatgtta ggtattctcg cctcttcgaa gcggaagcaa ttgttttgaa
     4681 gggtccggca agtgggcgag gcgaagaaac ggtgattacc ggacgattgc cgtggatcta
     4741 ggaggactgt cgatgaacat tgccatcaca tttcggcagc ttgaggcaag cgaagcggtg
     4801 aagcagtacg ctacggacaa agtggcgaag cttcaaaagt tccttcgtaa gccgctcagc
     4861 gcacaggtga cgttgagcct ggacaagctg gatcacgttg ccgacatcca tgtgcatgcg
     4921 ggcagtgacc actttcacgg aagtgagcga agcgaggata tgtacgcttc gatcgataag
     4981 gtgattgata agttggaccg tcagattcga gggtccaagg gggttcttca aaccaaaaaa
     5041 cggggctccg aggggctcaa ggcccgcgaa gtcatcgagc ccaaggactg accggaagcg
     5101 tcctacattc caccattcat tttcctaatc gcatcctcga ccactcatcg cgcatgtcgt
     5161 atgggtttgt caaagctcgg gtcggtgagc cgagtagttg gtgatcagcg gcaaaaccag
     5221 ccggtctgct gcgagaaccc gatggaatca cagcgacatg cgtcttacgg atattttgac
     5281 ggaagaccgc gtgcgagtga cgcggcatca agacggcatc aaggtcaaga gcgaagttct
     5341 cgaggcgctt tcgctccttc tagcgtccgg cacagggcaa gcggcggagt tgattcataa
     5401 aaccctgacg gaccgagaga gtcttcaatc gacgggaatt ggcgatggtg tggcgatccc
     5461 gcacggctcg ctcgaagcgc ttgtcacgca aaccgcggcc atcgttctat gcccggatgg
     5521 ggtcgatttt cattcgatcg atgggcgtcc tgcaaaaatt ttcgttgggg tgattggacc
     5581 tcggggttcc accggggagc atctacgaat gctagcgcgc atttcccgtt tgctccggga
     5641 tggaaatcta cgagatcgtt tgctggaagc ttcagatggt acccaagcat tcgacgtcat
     5701 cgtcgaggaa gaaagggggc ggccatagat cttctcgatc cgctggagcc tttatccgtg
     5761 cgcgagttat tcgccgacgc gaaattggga ctgtccattg tggagatggt gggtcggcag
     5821 ggagacagcg cgcaggtcat tcacaaccgc attcaacggg tcggtttggc gctcgcgggc
     5881 catttccatg gcgtggttcc ctctcgcgtg caggtttgtg gcgaaacgga aatctcctat
     5941 ctgaagacca tgacatcgga acaacgctcc gcggctctcg gagctttcct ggcccttcag
     6001 gtctgcagtg tcgtggtcac cggaggtctt ccgctacccg aggaaatgat cgaacaggcc
     6061 gaacgaaatc gttgccttgt cgtgcgaatt tccgaacgca cgggccatgt gattcaggcc
     6121 atcgaaggat atctcgatga gcgtttggca ccacgcatgc agcttcacgg tgtgctcgtg
     6181 gatgtttttg gcgtaggcat gctcctgctc gggaaaagcg gaatcggaaa aagtgaatgc
     6241 gcgctcgacc tggtactacg cggtcatcgc ctcgtcgccg atgattgggt attgtgcgac
     6301 ttccggcatc cgggccatgt gtttggggct ccggccagtc ttcttcgtca tctcatcgag
     6361 gtacgtgggc ttggcattct caatgtgaaa acactgttcg gagtcacggc tgtccgagac
     6421 cgaaagcgca tcgacatcat tgttcgatta tgctctgcca cggatgaacc ggaacaggag
     6481 cggctaggaa ttgaagaccg ctttcatgag ttgttgggcg taaaaattcg agaaatcgcg
     6541 cttcccgtcc gtgcggggag ggacatggcg gccatcgtgg aaatcgccgc ccgcgacgag
     6601 ttgtctcgtc gggccgggca tcattccgcc aaggagtttt atgatacgat tgagcgcagg
     6661 cttcaacacg aaggtaattc atgaccaacg attcctctcg cgcttctgat tcgaacggac
     6721 aactcgctga cccaaagatt ttcgagcgta tcgtcattgt cagcggaatg tctggcgcgg
     6781 gaaaatcgac ggccttgcat gctctggaag acctcggata cttctgtatc gacacgcttc
     6841 ccaccacggt gatcgagccg gttttggaag cttgtcgaaa cgcgtcggtg aagtggattg
     6901 ctcttggaat cgacgtccgt gttcggatgt ttctcgagca tttcagttcc gcgctcgatc
     6961 tgattcaaag cacacccaac gtcgatcttt cggtgttatt tctcgatgcc agcgaccaga
     7021 cgttgctaag ccgcttcagt gcgacacgtc gtcctcatcc tctatccacc aatgtgcaag
     7081 ggccggattg tggagccatg gccttgttgg acggaattac ttccgagcgc cagcggttgg
     7141 cgcgggttcg ggttcgcgca accgatgtca tcgacacgac gggaatgacg gtacatgact
     7201 tacggcgtcg cgtcgtggag ttgctcggcg gtgaaacgtc gcgggggcgg atg
//
LOCUS       DAOSOR010000284         7242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10332_length_7242_cov_4.337693, whole genome shotgun sequence.
ACCESSION   DAOSOR010000284 DAOSOR010000000
VERSION     DAOSOR010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7242
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10332_length_7242_cov_4.337693"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="PK710_14835"
     CDS             <1..919
                     /locus_tag="PK710_14835"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOT11052.1"
                     /translation="DLIRAGGKRMRPSIVLLATRLVCGADIRSDQVLGFAAAVEMLHT
                     ATLLHDDVIDEAPLRRGKPTARMLWGNPVSVIGGDLLMVRALNVINALGKPFLDRMTV
                     QTLQELVMGEVEQLERRGKLEMDIDTFERIAHRKTASLFVLGAVGGAFLAGASQPILD
                     HLRQFACAAGLAFQLDDDLLDLCSTPEAIGKAVGQDLSTGSVTLPVSDVLNRDPALRI
                     AISDHRSATPQEPLPPWICHRLLEIAHQPGVLDRCRNLVRSYGNRSLDALLELQPSTE
                     RRALETLVELLVWRSAGQHDVQDCKRELP"
     gene            916..1842
                     /locus_tag="PK710_14840"
     CDS             916..1842
                     /locus_tag="PK710_14840"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOT11053.1"
                     /translation="MKEITVPTGLHAWLLALRAYSLTASVMPVLLAVGLARALDLDVS
                     PWFAGLVVATALSLHLGTNLINDAVDLWKGVDRPGTTGGSGMLTNGALDPKSVFRVGM
                     GFLVLAALVGIPVIMVRGWPVLVMGLLGALGGYAYTAGPGYKYKALGDLGVFALMGPL
                     LVVFSLYALTGVTEASVLLRALAASVPLGLLVTAILAANNVRDFHDDAEAGIRTLAHV
                     MGIRWARRYVMMLFVLAFAVTLGIVLTGVTPWSVVLGLAAMVPAVGVLRDLVTSTDGL
                     ELGKRMVVERTAKVHAVYGAFVFLSLMIWPKW"
     gene            2096..3298
                     /locus_tag="PK710_14845"
     CDS             2096..3298
                     /locus_tag="PK710_14845"
                     /inference="COORDINATES: protein motif:HMM:NF009555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOT11054.1"
                     /translation="MNADKVRELAKRDEAAVVQFLRDIVAIPSFSTQEENVVKRIAKE
                     METVGFDEVRIDAVGNVIGRMGNGPVKIMFDNHIDTVGVGDPASWKGRDPFDPKLENG
                     KIWGRGVVDEKAAMASTVYAAKIIKELGLGDQCTVWVVGSAIEEDCDGLTQLHLIQNE
                     GIRPDYVVLGEPTDLNVYRGHRGRVEMIITIKGRSAHAAHCEKGVSALYKAARILLDI
                     EKLNDRLAHDDFLGKGTITASFMDVKTASLNSVPNAAIIYLDRRLTVGETKESALEEI
                     RALPSMGDAEVEILQYEATAWTGKKVEQDKYFPTWVLDEQHVLVQAAVEAIEAARGTK
                     PKVSRWVFSTNGVATMGRLGIPTIGFAPGLEELAHTTDEYINVADLVDAVAGAALIPQ
                     TLARRLAK"
     gene            complement(3358..4692)
                     /locus_tag="PK710_14850"
     CDS             complement(3358..4692)
                     /locus_tag="PK710_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11055.1"
                     /translation="MLRESFIVGTLVLSFTMLGCPSQDPVRPKEEAGKSPNTTQLPSP
                     LSSISTPTPRDAGYEGPVGIPADSRGRLVIRDAQPQEPTPLVPARPLDADRLANREMQ
                     GLTMTAEWTWEGVAAAPGGAEVNQPGIKAARAATRHLWQIELLEAGRLRIVFDSVSYP
                     LTKYTELRARHDAYGHILVWPEADNYRVIPSGALRALLDERRVDTSPLVAGRTDKEQP
                     GRPYFGFPTRSVGVSTPWGKLDLVQARTSNAGSGGVLFCRLLLELIGARPDSPVCEEG
                     RVPVRASYAWKDGGKVVLEVSTLMIRPDFQPTMFMIPPSRATFTDVGLPPDATGLFLT
                     REQMEAFRSRPIEPATRSDRESKGAPGEGFFAVNRTDALRFLCIDGVPVTWVPAHGEQ
                     YVIGSRPGKYTAQWRSFLGTSIEPPTEVLIPARIVLGEEPEEAGAPEVDAAL"
     gene            complement(4694..5602)
                     /locus_tag="PK710_14855"
     CDS             complement(4694..5602)
                     /locus_tag="PK710_14855"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOT11056.1"
                     /translation="MDLQVKIGELSLSNPVLTASGTFGYGIEFESFFDISRLGGICTK
                     GLSLLPRVGNPPPRIRETASGMLNAIGLANIGVEAFCKDKLPRLRELGATVIPNLYAS
                     SVEDFIALAERLDREEGIAAVEVNVSCPNVTKGGLAFGVDPVAAAGVTAAVCKATRFP
                     VWVKLSPEAGRITDVACACEEAGAQALSAINTIRGFAIDTETWRPLLANRTGGLSGPA
                     IKPIAVRMVWEICQAVRIPVIGVGGIASAEDAIEFMLAGASAVQVGSANFRDPMASMT
                     VLEGISAYCERKGLAAKDLIGKVVLD"
     gene            complement(5615..6409)
                     /locus_tag="PK710_14860"
     CDS             complement(5615..6409)
                     /locus_tag="PK710_14860"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11057.1"
                     /translation="MEQYDRRPARQVTVPLVRRDSIGASHHVLTFEYDTPLEAQAGQF
                     VMVRGTNWGQSPFLPRPMSLLHGGKRPSILVKVVGEGTRRLANAEPGDLFSVLAPLGR
                     PWSPCSSHRVPILVAGGVGVCPLLFLARTLKQQGIRPVALYGGRSSGDLVLYEELQAE
                     SDLRLATEDGSAGVKGKVTALLDEALMEADGRATVYTCGPRAMMASVVAAARKANVPC
                     EVSLETVMACGYGVCLGCAVPRFDGGYVYACSEGACIDGRVIDWKK"
     gene            complement(6373..6939)
                     /gene="rimI"
                     /locus_tag="PK710_14865"
     CDS             complement(6373..6939)
                     /gene="rimI"
                     /locus_tag="PK710_14865"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOT11058.1"
                     /translation="MFRRLSSPLDVTIRALRSTDSAVPRVQQLDNRCFQGGERSDIVA
                     EIDRPFTYLWIAALSADPQCVVGYILTWHVADELHILNVATDPDHRGLGVGHALVAQA
                     IAWAGQHHVQSVLLEVRRSNLAALRLYRSFGFFLRGLRKEYYGDGEDAVEMGLVLDPK
                     TGDIVASRDEVRLEEVSWSNMTGVLHDK"
BASE COUNT         1541 a         2183 c         2011 g         1507 t
ORIGIN      
        1 ggacttgatt cgcgccggcg gaaaaagaat gcgcccctcg atcgttctgt tggctacccg
       61 actcgtttgc ggagccgata ttcgttccga ccaggtcctt ggattcgctg cagccgtaga
      121 aatgcttcat acggccacgc tccttcatga tgacgtgatc gatgaggccc cgttgcgacg
      181 aggcaaaccc acggcaagaa tgctttgggg gaaccctgtt tccgtcatcg gcggggactt
      241 gctgatggtg cgtgcgctca atgtcatcaa cgccctgggc aaaccgtttt tggataggat
      301 gacggtgcag actctgcagg aactcgtgat gggagaagtc gaacaactcg aacgtcgggg
      361 aaagctcgag atggatatcg ataccttcga gcgaatcgcg catcgaaaaa cggcatccct
      421 tttcgtattg ggtgccgtag gaggcgcgtt tttagcaggt gcttcccagc ctatcctcga
      481 tcatctccgg caattcgcct gcgcggccgg actcgccttt caactcgacg acgacctgct
      541 cgacttatgt tccacacctg aagccattgg caaagccgtg gggcaagacc tttcgaccgg
      601 ttcggtgact ctgcccgtat ccgatgtgct caaccgcgac cccgccttgc ggattgccat
      661 ttccgaccat cgaagcgcca caccccaaga acccttgccc ccctggatct gccaccggtt
      721 gctcgaaata gctcatcagc cgggtgtgct cgaccgttgc cgcaacctgg ttcgttccta
      781 cggcaaccgc tcgctcgacg ccttgctcga actccagccc tcgaccgaac gtcgtgcgct
      841 cgaaaccctc gtcgaactgc tcgtgtggcg atcggcggga cagcatgacg tccaggattg
      901 caaaagggaa ttgccatgaa agaaataacg gtgcccacgg gactccatgc gtggttgttg
      961 gcgcttcggg cctattcatt gaccgcatcg gtgatgcccg tgctactcgc ggtgggattg
     1021 gcgcgggctc tggacctcga cgtttcccca tggtttgcgg gcttggttgt ggccacggcc
     1081 ctgtcgttgc acctgggaac gaatttaatc aatgatgccg tggacttatg gaaaggcgtg
     1141 gaccggccgg gcacgaccgg cggtagcggg atgttgacca acggagcgct tgatcccaag
     1201 tccgtgtttc gtgtgggcat gggttttttg gtgttggcag ccctggttgg cattcctgtg
     1261 atcatggtac ggggctggcc cgtgctcgtc atgggcttgt tgggcgcatt gggtgggtat
     1321 gcctacaccg ccggccccgg gtacaagtac aaagctctcg gggatctggg agtatttgct
     1381 ctgatgggcc ccctgctcgt ggtcttttcc ttgtacgcac tcacgggcgt caccgaagcg
     1441 agcgtgttgt tgcgggcact ggccgcttcc gtgcctcttg gcctgctcgt gacagcgatt
     1501 cttgcggcca acaacgttcg tgacttccac gacgatgccg aagcgggcat tcgaacgctg
     1561 gctcacgtca tggggatccg ctgggcccga cggtatgtca tgatgctttt tgtcttggcg
     1621 tttgcggtca cgttggggat cgtcctgact ggtgtgacgc cctggtccgt ggttttggga
     1681 ctcgccgcca tggtgcccgc ggtgggggta ttgcgagatc tcgtgaccag caccgatggt
     1741 ctggaactcg gcaaacgcat ggtcgtggaa cgaactgcca aagtccatgc cgtctatggc
     1801 gctttcgtct tcttgagtct catgatttgg cctaaatggt gaacaaattc aattgcttca
     1861 ccgtttttcg gcagggggga agaccaatcg atccgttgtg atttcgctcg cttgcgtgta
     1921 caagaccggg ccggagccga acgtggtgtt cggcgtattc ctgacgaacg gaagcttgga
     1981 gaccatggtc ttctggggcc gcgcgaaccg ttgtttcgcc ggtcgagcca ggtggctctc
     2041 cgagtacaag cgagaaatgg acgatgagtc catggttatg tgtgaggtga atcagatgaa
     2101 cgcggataag gttcgagagc ttgcgaaacg cgatgaagct gctgtcgtgc agtttcttcg
     2161 ggacatcgtt gccattcctt ccttctccac ccaagaggag aatgtcgtca aacgtatcgc
     2221 caaagaaatg gaaaccgtcg gcttcgatga ggtccgaatc gatgcggtgg gcaatgtgat
     2281 cggtcgcatg ggcaatggcc ctgtgaagat catgttcgac aaccatatcg ataccgtagg
     2341 cgtgggcgat cctgcctctt ggaagggacg agatcctttc gatccaaagc ttgaaaacgg
     2401 aaaaatttgg ggccgcggtg tcgtcgatga aaaagcggcg atggcaagca cggtctacgc
     2461 tgccaagatc atcaaggaac ttggactcgg agaccaatgt acggtgtggg tggtgggctc
     2521 cgccatcgaa gaggattgtg atgggttgac gcaactgcat ctcattcaga acgagggaat
     2581 tcgcccggat tacgtggtgc tgggagagcc gacggacctc aacgtctatc gcgggcaccg
     2641 tggacgcgtg gagatgatca tcacgatcaa agggcgttcg gcgcacgcgg cccattgcga
     2701 aaaaggtgtg agcgccttgt acaaggcagc tcgtattctg ctcgatatcg aaaaactcaa
     2761 tgatcgactc gcccacgacg atttcttggg caagggaacc atcaccgctt cgttcatgga
     2821 cgtgaaaact gcttccctca attcggttcc caacgcggcg atcatctact tggaccgtcg
     2881 cctcacggtc ggcgaaacca aagaaagcgc actcgaagaa atccgtgcgc taccaagcat
     2941 gggcgacgcc gaggtggaga ttttgcaata cgaagcgacc gcctggaccg gcaaaaaagt
     3001 agagcaagac aagtactttc caacatgggt gctcgatgag caacacgtgc tcgtgcaagc
     3061 ggccgtcgaa gccatcgaag cggcccgtgg gaccaaaccc aaggtatctc gttgggtgtt
     3121 ttccaccaac ggtgtggcta ccatgggacg cttgggtatc cccaccatcg gatttgctcc
     3181 tggactggaa gaactggccc atacgacgga cgaatacatc aacgtcgcgg acctggtcga
     3241 tgcggtcgct ggggcagcgt tgattcctca aaccttggcg cgtcgcctgg ccaagtaatc
     3301 ttacgtgtgt gcctaaggag ggtcggtcct atatccggcc ctttttcatg cgaaacctca
     3361 gagagccgcg tccacttccg gcgctcctgc ctcttccggc tcttcaccca agacaatacg
     3421 cgcggggatg agaacctccg tgggtggctc gatgctcgtc cccaaaaaag accgccactg
     3481 tgccgtgtac tttccaggcc gtgacccaat gacatattgc tcgccgtggg ccggaaccca
     3541 ggtcacaggc acaccgtcga tgcaaagaaa tcgaagcgca tccgtacgat tcaccgcgaa
     3601 aaatccttct cccggcgccc ccttgctttc tcgatccgaa cgagtcgcag gctcgatcgg
     3661 acgcgaccga aacgcttcca tttgctcccg tgtcaaaaac aaacccgtcg catcaggcgg
     3721 taaacccacg tctgtgaatg ttgcccgcga aggcggaatc atgaacatcg tgggctgaaa
     3781 gtccggtcga atcatcagcg tactcacttc cagcacaact tttccaccgt ctttccaagc
     3841 ataagaagcg cgaacaggga ctcgaccctc ttcgcatacg ggcgagtcgg gccgcgcgcc
     3901 aatgagttcc aaaagcaaac gacaaaacaa aactccgcca gaacccgcgt tggacgtccg
     3961 cgcctgcacc aaatcgagct tgccccaagg cgtggatact cccacgctac gggtcggaaa
     4021 cccaaaatag ggacgcccag gttgctcctt atccgttcgt cccgccacca agggactcgt
     4081 atccacacgc cgttcatcca acaacgctcg caaggctccc gagggaatga cacggtagtt
     4141 gtccgcctcc ggccacacca aaatatgacc ataagcatcg tgacgagcgc gaagctccgt
     4201 atacttggtg agcggatagg atacggaatc gaaaacgata cgcaggcgtc ctgcttcgag
     4261 aagctcgatt tgccatagat gacgagtggc cgcacgcgcc gctttgatgc ccggctgatt
     4321 cacctcggct ccccccggcg ccgcggctac tccctcccat gtccactccg cggtcatggt
     4381 aagaccttgc atttcccggt tggcaagacg atcggcgtcg agcggtctcg caggaaccag
     4441 cggcgtaggc tcttgaggct gcgcatcgcg aatcaccaaa cgcccccgtg agtccgcggg
     4501 aatacccacg gggccttcgt atcccgcatc tcgcggtgtc ggagtagaaa tcgacgacaa
     4561 aggtgacggc agttgcgtgg tgttcgggct ttttcccgct tcctctttcg ggcgtacagg
     4621 gtcctggctg ggacatccca acatcgtgaa actcaacacc agggtcccaa caatgaatga
     4681 ttcgcgtagc atttcagtcc aacacgacct tacctatgag gtccttcgcc gcaagccctt
     4741 ttcgctcaca atacgcagaa ataccttcca ataccgtcat cgacgccatc ggatctcgaa
     4801 agttcgcgct ccccacttga accgccgatg cccccgcaag catgaactcg atcgcatcct
     4861 ccgccgaagc tatcccccca acgccgatga caggaatgcg taccgcctgg catatctccc
     4921 acaccattcg caccgctatc ggcttgatgg ctggacccga taaccccccc gttcgattcg
     4981 caagcaatgg acgccatgtc tcggtgtcga tcgcaaaccc acgaatcgtg ttgatggccg
     5041 acaaagcttg cgctcccgcc tcttcgcatg cacacgccac atcggtgatt cgacccgctt
     5101 ccggtgaaag cttgacccat acgggaaaac gcgtcgcttt gcagaccgct gccgtaaccc
     5161 ccgccgcagc aaccgggtcg accccaaacg caagcccccc cttcgtcacg ttgggacaag
     5221 atacattgac ctccaccgcg gcgattccct cttctcgatc cagacgctcc gcgagcgcga
     5281 tgaagtcttc cacactcgat gcatagagat tcggaatgac cgttgcccca agctcgcgca
     5341 agcgaggaag cttgtccttg caaaacgcct ccacaccaat gttcgcaaga ccaatagcat
     5401 tgagcatgcc cgatgccgtc tcgcgaatac gaggtggtgg attgcccacg cgcggcaaaa
     5461 gactcaaccc tttcgtgcaa atacctccga gccgcgatat gtcaaaaaag ctttcaaatt
     5521 caataccata gccaaatgtc cccgatgccg tcagcacggg attcgatagg ctcaactccc
     5581 caatcttcac ctgcaaatcc acgtccctgc ctcctcactt cttccaatca atgacccgtc
     5641 catcgatgca agcgccctcc gagcaggcgt agacataccc accgtcgaac ctgggaacgg
     5701 cacagcccaa acatacgccg tatccacatg ccatgaccgt ttccaaagat acctcgcagg
     5761 gaacgtttgc cttccgagcc gcagccacca ccgatgccat catggcccga ggaccgcagg
     5821 tatatacggt ggcgcgtcca tcggcctcca tgagcgcttc gtccaaaaga gccgtcacct
     5881 ttcccttcac acccgcactg ccatcctcgg tggcaagacg aagatcgctt tcggcctgca
     5941 actcttcgta caaaaccaga tccccgctcg atcgtccacc gtacaaagca acgggtcgta
     6001 tcccttgctg tttcaaggtt cgcgcgagaa ataaaagggg acatacgcca acaccaccgg
     6061 ctaccaatat cgggactcga tgcgatgagc atggcgacca ggggcgtccg agaggagcta
     6121 gcaccgagaa caggtcccca ggctcggcat tggccaaacg ccgggtgccc tcacccacga
     6181 ccttgaccag gatggaaggg cgctttcccc cgtgaagcaa actcatggga cggggcaaaa
     6241 aaggtgattg tccccagttc gttccccgca ccatgacgaa ttgccccgcc tgggcctcca
     6301 acggcgtgtc gtactcgaac gtgagaacgt gatggctcgc cccgatcgaa tctcgccgaa
     6361 cgaggggaac cgtcacttgt cgtgcaggac gcctgtcata ttgctccacg aaacctcctc
     6421 gagccgcact tcgtcacggc ttgccacgat gtcccctgtt tttggatcga gcacaagccc
     6481 catctccacg gcgtcttccc catcgccgta atactccttg cgaagccccc gaagaaaaaa
     6541 accaaaggag cgataaagac gcagcgccgc gagattactt cgacgtactt ccaacaacac
     6601 cgactgcacg tgatgctgac ctgcccatgc gatcgcttgg gctacgagcg catggccgac
     6661 acccaaccca cgatgatcgg ggtccgtggc cacattcaaa atatgcaatt cgtcggcgac
     6721 atgccaggtc aggatatagc ccacgacgca ttgggggtca gcggatagcg cggctatcca
     6781 taagtatgtg aaaggtctat cgatttcggc gacgatatcc gagcgctccc cgccttgaaa
     6841 gcagcggttg tcgagttgct ggacccgtgg cacggcacta tcggtggagc gtagcgccct
     6901 gatggtgaca tcgaggggcg aggatagacg gcgaaacacg gcggcagcct accctgggat
     6961 tcgctgtcca accagcttct ttccttggaa atccattgga ttggtttgca tgggatgaat
     7021 gcgtttccag ggtggagcgc gagccgacga tgcgctaggt tctgttcgtg acgcgcgttg
     7081 ccaccttgtt gatgtcgatc ggtttgcttt cgttgggtac aacgggttgt caggatatct
     7141 cgcggtttgg aaccgggccg gggcagagct attgcggtcg tatcgttccc ggtgggttcg
     7201 tacgggaagg cttttctccc ggggtgacga tgcgcgttca tc
//
LOCUS       DAOSOR010000285         7180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10438_length_7180_cov_4.734456, whole genome shotgun sequence.
ACCESSION   DAOSOR010000285 DAOSOR010000000
VERSION     DAOSOR010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7180
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10438_length_7180_cov_4.734456"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PK710_14870"
     CDS             <1..298
                     /locus_tag="PK710_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11059.1"
                     /translation="RIMGQMKKNNHLDPDIFDLFVRAGVYRVYAQRFLSPEQIDEVDE
                     QALLSIVPRPFDCLPTHEQEMRKNTFLPEYEDQRHRSVHPPPVTVPSEWSFHSK"
     gene            complement(404..1267)
                     /gene="hflC"
                     /locus_tag="PK710_14875"
     CDS             complement(404..1267)
                     /gene="hflC"
                     /locus_tag="PK710_14875"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HOT11060.1"
                     /translation="MERKFYVFALLLLFAAGIAKSSAFVVDETEQVIITQFGQYKRTI
                     SEPGINFKLPYIQTVERMERRILSSDARSDKYLTLDKKWLVADPITRWRIADPLKYFM
                     TVHDEARARQNLDDIVLSEMRREIASHNFGDIVGNARDPLMIAVAERASSQVKDFGID
                     VIDVRIKRADLPTEVQESVFARMRAERERIAKKYRSEGDEEAAKIRADTDKDKTIILA
                     KAYEEAEKLRGEGDAISTKIYAEAYGKDPEFYTFLRSLTAYEQLLNAQTSVVLSTDSP
                     LFKYLDNPSKK"
     gene            complement(1269..2270)
                     /gene="hflK"
                     /locus_tag="PK710_14880"
     CDS             complement(1269..2270)
                     /gene="hflK"
                     /locus_tag="PK710_14880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HOT11061.1"
                     /translation="MRSQFGRNGGWKRPDSSNIEQVSEAVRRNKRKLGPIILGVLLLI
                     AILTGVYSVNPGEQGVVRTFGKETGKTGPGLHFALPIVQKVDVVNVEQIRRVEIGFRG
                     KERVPSESLMLTGDENIVEAQVIVQYRVIDPSKYLFRLRDPEQTVLATAEVALRSVIG
                     RTTIDDAMTTGREEVQSETRSQLQTLMDTYQSGIAITEVKLQMVDAPDEVREAFHDVV
                     RAREEKEQKINKAKGYNEDLIPRARGEARQVVRAAEAYKQQRELRANGDVANFLAVLE
                     EYRKAEKVTRDRLYLETMERILRTIDKKTLVDRDVTKNALPVLNLGPAVVGGQAGGK"
     gene            complement(2459..2827)
                     /locus_tag="PK710_14885"
     CDS             complement(2459..2827)
                     /locus_tag="PK710_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11062.1"
                     /translation="MSQRTQKSGKKATQELRIAYKTRKGFEAAYTLISRPVTPADRKK
                     IREAETNGQVPGLGGLAHDCPWVWQVEDGLEEIQPETIQFCALLATVALGPIVPPHLD
                     TVLSVRTARDLAANPHHAYR"
     gene            complement(3056..7027)
                     /locus_tag="PK710_14890"
     CDS             complement(3056..7027)
                     /locus_tag="PK710_14890"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT11063.1"
                     /translation="MFAPHTAERFRLETRVGAGAMGEVYRATDLQSGEQVAVKFLHRK
                     ASREEAARFHREVAMLADLRHPGIVTYIDHGTWTDGRPYFAMEWLEGEDLGQLTRRQP
                     LGMSDAVEVVRRVAQAMAAVHARGVIHRDLKLTNIFVPGGNIKRGVKLIDFGVVKPAE
                     PDDFQTHPGSIIGTPHFMAPEQARSEPVDARSDVYSLGAVLFRLVTGRHVFETDHIIA
                     FLGRLVLEDAPLASSVRFDVPELLDKAIARTLARNPDHRPEDAGVLARWLARLPPMTN
                     EPPNSDQSASAIRRVPTHTSSSAESVSPALPPGAQERRVVGVVLASLPSGSLSDELAG
                     KIRAILGPDTRLESLQGGQLVAGLGLESTRGDEAVRAARAALMIATTVPEARVAVASG
                     HAVAGRRGIAGEALEQAAIQLDRAPPGGIRIGKATRPLLQGRFVVRIDDIGAVLLHED
                     IAASDRMQLLGVKTPTLGRDSEIELLLSTFRQVTQDASPRAVIVTGPTGVGKSRLRFE
                     TIRRLEKSVDSLDVLMARGDPMQTRMGLSALGRALRARMGIRDGEPAALQMERVTRYV
                     RQRDATYQDAEAFLGEFVGVLFSDVASEPLRAARSAPQLMTARILQVLETLIRHDARH
                     VPQVIVLEDLHLIDDTTREMIDWLLGSRDLRLVVLGFGRDRSVDALRDLWAKRSVTRV
                     GLAPLPYSVCEQIASMVLPHLDETRRETILERAEGNALFLEELLRNAADGHDDLPVSV
                     QALIQARLDQLSPELRHVVRAATIFGRQFWTDGVSNLIGRDVEQDLQALARVELIAQH
                     DTSRIDGQVEWVFPQNVIFETAYSSLIERDRIALHRTASEWLLIAGEEDIGTIARHAE
                     AGQDMARAAVLYNRASGQAYGNGQLEAALDFADAAIRCGTDATTQAQALFQRAQILSW
                     LGRYEDQLEAAEKAISFAEQGTESWGEAHRLAASAMRELGRPSDAEARFAWALDNPFL
                     DRLSLAAQAKLHAERTRALADMGRAKEGARSAERAIRLAHAAGESGTHAMLRALDARF
                     MATAFLGDFSASIEAAISVVDNADRVGDAVLATRARINLGFVLTRVGVFEQAREQLER
                     ALTDTRLLRMRAGEGFALHNLGMCFARLDKIDRGIQLEKEAREIGEETLHFRLKLNSR
                     IYEAVMLTWRGEPGDLRDALMTIEACRAEANDHPVSYVEATSVLAQVHRARRDLSACL
                     IACEDALEKLATIGAMEEGEEMLWLTYSETLLDLGQGEKAQSAIHHAYRCVMDRCGQM
                     SDQHHQQAYLSRLYECRRIVDLAEMYLGYPRPQIEQESQEPAVPRHSVRPKSIVRTSD
                     PPR"
BASE COUNT         1532 a         2041 c         1989 g         1618 t
ORIGIN      
        1 gcgcatcatg gggcagatga agaaaaacaa ccatctcgac cctgatattt tcgatctatt
       61 cgttcgagcc ggggtctatc gagtttatgc gcagcgtttt ctatcgccgg aacagatcga
      121 cgaagtggac gagcaagcgt tgttgtcgat cgtgccgcga cctttcgact gccttcccac
      181 gcacgaacaa gagatgcgaa aaaacacgtt tttgccggaa tacgaggatc agcgtcatcg
      241 gtccgtacac cctccacccg ttaccgttcc ttccgaatgg tctttccatt caaaatgaga
      301 gcctggcagc ggcctagtgg tgatcgcgcg cgtagctgtt tcaaggcagg aaatgaaatg
      361 acccgatgag ccaacaacgc ggcggaagag tcatcgggcg tcgctatttt ttcgaggggt
      421 tatcaaggta cttgaacagg ggggagtcgg tggacaagac caccgaggtt tgagcgttga
      481 gcagttgctc ataagcggtc aagctacgca aaaaagtgta aaattccggg tccttgccat
      541 aggcttccgc ataaattttc gtgctgatgg catcgccttc gcctcgaagc ttttcggctt
      601 cctcgtaggc tttcgccaaa atgatggtct tgtccttgtc cgtgtccgct cggatcttgg
      661 cagcttcctc atcaccctcg gaacgatatt ttttggcaat gcgttcacgt tccgcccgca
      721 tgcgggcaaa cacgctttct tgcacttccg tgggcaagtc cgctcgtttg attcgaacgt
      781 cgatgacgtc gataccgaaa tccttgacct ggctcgaagc ccgttccgct accgcaatca
      841 tcaacggatc gcgagcgttg ccaacgatat caccgaagtt atgactcgcg atttcgcgac
      901 gcatctcgga taacacgata tcgtcgagat tctgtcgcgc gcgggcttca tcgtgaaccg
      961 tcatgaaata tttaagcgga tcagcgattc gccagcgcgt gatgggatcg gcgacgagcc
     1021 attttttgtc taacgtaaga tacttgtcgc tgcgtgcgtc gctgctcagg atgcgtcgct
     1081 ccatgcgttc gacagtctgg atgtagggga gtttgaagtt gatccctggc tcgctgatgg
     1141 tccttttgta ttgtccaaat tgcgtgatga tgacttgctc ggtttcgtcg acgacaaaag
     1201 ccgaggattt tgcgatgccg gcagcgaaga ggagtaacaa agcgaagacg tagaatttgc
     1261 gttccatatc acttgcctcc tgcctggccg cccacgacag caggtccgag attcagcacg
     1321 ggaagagcgt ttttggtgac gtcgcgatcc accaaggttt ttttgtcgat ggttcgcagg
     1381 atgcgctcca tggtttcgag atagaggcga tctctcgtga ctttttctgc ctttcggtac
     1441 tcctcgagaa cggcgagaaa gtttgccaca tcgccattgg cgcgtagttc ccgctgttgt
     1501 ttatacgctt cagccgctcg gacgacctgt ctggcttcgc ctcttgctct ggggatcagg
     1561 tcttcgttgt agcctttggc cttgttgatt ttctgttctt tttcttcccg ggctcggacg
     1621 acgtcatgga aggcttcgcg gacttcgtcc ggggcatcca ccatttgaag cttaacttct
     1681 gtgatggcga tgccgctttg gtaggtatcc atcaacgttt gtagttggga acgcgtttcg
     1741 gattgcacct cctcacggcc cgtcgtcatc gcatcgtcga tggtggttcg tccaatcacg
     1801 cttcgaagcg cgacttccgc ggtggcaagc acggtttgct cggggtcacg caagcgaaac
     1861 agatacttgc tgggatcgat gacgcggtat tgcacgatga cctgtgcttc gacgatattt
     1921 tcgtcgccgg tgagcatcaa tgattcggaa ggcactcgtt ctttgccgcg aaatccgatt
     1981 tccacgcgac ggatctgttc gacattgacg acgtcgactt tctgaacgat gggaagagcg
     2041 aagtgcagac cggggccagt ttttcctgtc tctttgccaa aagttcgtac gacgccttgc
     2101 tcaccgggat tgacgctata cacaccggtc agaatggcga tcagcagcaa aacgccgaga
     2161 ataattggtc caagcttgcg tttgttgcgt cgaacggctt ccgatacttg ctcgatattg
     2221 ctggaatccg ggcgtttcca gccgccgttt cttccgaatt ggcttcgcat gatgttgctt
     2281 tcggtttgtt caagaggcct aaggacgatg ggcacgcgtg gctggcgctc gatgcatggc
     2341 gctaccaaag cgtggcgaat ggacgcaaat cgtcgtggct caccctgacc cacggacggt
     2401 tcggagcgca agggctatca gcattggacg gcggaaggat tccagattcg gggaggcatt
     2461 agcggtaggc gtgatggggg ttggcagcga ggtctctggc ggttcgaacg ctcaacacgg
     2521 tgtcgagatg gggcggcacg atcggtccca gggccacggt cgccaaaagc gcgcaaaatt
     2581 gaatggtttc aggctgaatc tcctccagtc cgtcttccac ttgccaaacc cagggacagt
     2641 catgggcgag cccaccaagc ccaggcactt gcccattcgt ttcggcttca cgaatttttt
     2701 tgcgatctgc aggagtcacg gggcgtgaaa tcaacgtata ggctgcttcg aagcctttgc
     2761 gtgttttgta cgcgatacga agttcttgtg tggctttttt gccggacttt tgggtgcgtt
     2821 ggctcacgac cgacaaggaa gcagaaaaat atttttcccc gtggctcaaa acgaggggtt
     2881 catcgatttg cgcctggggc aacgagcaaa acaccaaata aatcacgctg tagtttcagc
     2941 acgaagggct agttgacgca atggttggtg gaagcgaaag accggacgtt gcgccgttgc
     3001 caccaggccg gaaagaggga aacactgcga aattattagt gtttttggga tggtactagc
     3061 gaggaggatc ggaggttctg acgatcgact tgggacggac cgagtgtcgt ggcacggcag
     3121 gctcttgaga ttcctgctcg atttgtggtc gagggtatcc gagatacatc tccgccaagt
     3181 ccacgatcct tctgcattca tacaggcgag acaggtaggc ttgctggtga tgttggtcac
     3241 tcatttgtcc gcatcgatcc atcacgcagc ggtaggcgtg gtggatcgcg gattgagctt
     3301 tttccccttg cccgaggtcg aggagcgttt ccgaataggt gagccaaagc atctcttcac
     3361 cttcttccat ggctccaatc gtggcgagtt tctccaacgc atcttcacaa gcgatgagac
     3421 aagccgaaag atcgcgacgc gctcgatgca cctgcgccaa taccgatgtt gcctcgacgt
     3481 aggatactgg atgatcgttc gcttccgcgc gacaggcttc gatggtcatc aaggcatcgc
     3541 gaaggtctcc tggttcgcct cgccaggtca acatgacggc ttcatagatt cgcgagttga
     3601 gcttgagccg gaaatgtaga gtttcttctc cgatttcccg ggcttccttt tccaattgaa
     3661 tcccgcggtc gatcttgtcg agccgcgcga aacacatacc caagttgtgc aaggcgaagc
     3721 cttcgcccgc tcgcatgcga agcaatcgcg tatcggtcaa ggcgcgttcg agttgctctc
     3781 gcgcttgttc aaagactccg acgcgtgtca agacgaagcc gagattgatt cgcgctcgtg
     3841 tggccaatac cgcatctccg actctgtcgg cattgtctac gacagagatg gccgcttcga
     3901 tcgaggcgga aaaatccccc aaaaaggctg tagccatgaa tcgagcgtcc aaggctcgaa
     3961 gcatcgcatg ggttccggat tcaccagcgg catgggcaag acgaatggcg cgctccgcgg
     4021 atcgagcacc ctctttggct cgccccatgt cggcgagcgc acgtgttctt tcggcatgaa
     4081 gtttggcttg cgccgccaac gagagcctat ccaaaaaggg attgtccaaa gcccaggcaa
     4141 aacgagcttc cgcatccgat ggacgtccga gttctcgcat ggcggaagcg gcaagacgat
     4201 gagcttctcc ccaggattcc gtcccttgct ccgcgaaaga aatcgccttt tccgcggctt
     4261 cgagctgatc ttcgtagcga ccgagccacg acagaatctg cgcgcgttgg aacaacgcct
     4321 gcgcctgcgt cgtggcatcc gttccgcatc gaatcgccgc atcggcaaag tccaacgccg
     4381 cctcgagctg tccgttgccg tacgcttgcc cagaggcccg gttgtataac accgccgctc
     4441 gcgccatgtc ttgcccagct tccgcgtgac gcgcaatcgt tccaatgtct tcctctccgg
     4501 cgatcaacaa ccactcggaa gccgtacggt gcaacgcaat gcggtcccgt tcgatcaacg
     4561 aagaatatgc cgtttcaaag atgacgtttt gaggaaatac ccattcgacc tgcccatcga
     4621 ttcgtgacgt atcgtgctgc gctatcaact cgacacgagc aagcgcttgc aagtcttgtt
     4681 caacgtctcg tccaatgaga ttcgatactc catcggtcca gaactggcgt ccaaaaatcg
     4741 ttgcggcacg aacgacatga cgtaactcag gggagagttg atcgagacgc gcttgtatca
     4801 acgcttgcac ggaaacgggc aagtcgtcgt ggccgtccgc ggcgttgcgg agcaactctt
     4861 cgaggaacaa ggcattgccc tcggcacgtt cgagaatagt ctcgcgacga gtttcatcga
     4921 gatggggcaa taccatcgag gcgatctgct cacagaccga atagggcagg ggagccaggc
     4981 cgacgcgggt gaccgatcgt ttggcccaaa ggtcacgcag ggcatcgaca gagcgatcgc
     5041 ggccaaatcc gagcacaacg aggcgaagat cgcggctgcc gagaagccaa tcaatcatct
     5101 cccgtgtggt gtcgtcgatg agatgcaaat cctcgaggac gatgacctga gggacatgcc
     5161 gggcatcgtg gcgaatgaga gtctcgagca cttgaaggat acgtgcggtc atgagttgcg
     5221 gtgcgcttcg cgccgcgcgc aacggttcgc tggccacgtc gctgaatagc acgcccacga
     5281 actcaccaag aaaggcttcc gcatcttgat aggtcgcgtc acgttgccga acgtaacgag
     5341 tcacgcgctc catctgcaaa gccgcaggct cgccatcacg aatccccatt cgcgcacgca
     5401 acgctcttcc cagcgcgctc aaccccatgc gcgtctgcat gggatcgccc cttgccatca
     5461 acacatcgag agaatccacc gatttttcaa gacgtcggat ggtctcgaaa cgcaaacggc
     5521 tcttgccgac accggtaggt cccgtgacga tgacagcgcg cggtgaagcg tcttgcgtca
     5581 cctgacgaaa cgtcgagagt aacaattcga tctcggagtc tctgcccagc gttggcgttt
     5641 tcacgccaag cagttgcata cgatccgatg cggcaatgtc ttcgtgcaac aacacggcac
     5701 cgatgtcgtc gattcgcacg acgaaccgcc cttgcaacag tggtcttgtt gcttttccga
     5761 ttcgaattcc gccgggaggg gcgcgatcga gttgaatagc cgcttgctcg agggcttctc
     5821 cagcaatccc gcgacgtccc gctaccgcat gtccggaagc gaccgcgaca cgagcctcgg
     5881 gaacggtggt tgcgatcatc agcgcggcgc gtgccgcacg aacggcttca tcgcctcgcg
     5941 tcgattccaa tccaagtccc gccacgagtt ggcctccctg caacgattcc aaacgcgtgt
     6001 ccggaccaag aatcgcccta atttttcccg caagttcgtc cgaaaggctt cccgatggca
     6061 acgatgcgag cactacaccc accacacgac gctcctgcgc accaggagga agagcaggcg
     6121 aaaccgattc cgccgaagac gatgtgtgcg taggaacacg acgaatcgcc gatgcactct
     6181 gatccgaatt gggtggctca ttggtcatcg gtgggagacg tgccagccat cgcgccaaga
     6241 ctccggcatc ctccggtcgg tgatccggat tgcgtgccaa tgtgcgagca atcgccttgt
     6301 ccaacaactc cggaacgtcg aaccgaaccg acgaagcaag aggtgcatct tcgagcacca
     6361 atctgccaag aaacgcaatg atgtgatccg tttcgaaaac atgtcggccc gtgacgagtc
     6421 gaaacagcac cgccccaagg gagtacacgt cggaacgagc atccacgggc tcactgcgcg
     6481 cttgctccgg cgccatgaaa tgcggcgttc cgatgatcga gcccggatgc gtctgaaaat
     6541 cgtcaggctc cgctggcttg accacaccga aatcaatcag tttcacccct ctttttatgt
     6601 tcccaccagg aacgaaaatg ttcgtcagct tcaaatcccg atggatgaca cccctcgcat
     6661 gaaccgcggc catcgcctgc gctacccgtc gtacgacttc caccgcatcc gacattccaa
     6721 gaggctgacg acgcgttagc tgcccaagat cctcaccttc aagccactcc atcgcaaagt
     6781 acggacggcc atcggtccac gtaccatggt cgatatacgt cacgataccc gggtgacgta
     6841 gatccgcgag catcgcgact tctcggtgaa aacgggctgc ttcctctctg gaggctttgc
     6901 gatgcaagaa cttgacggct acctgttcac cggattgcaa gtcggtggct cggtacacct
     6961 ctcccatcgc accggcgccc acgcgcgttt ctagtcggaa ccgctctgct gtatggggag
     7021 caaacactgt ccggagtcta gcggaaattg tcctccaggg gataggagcc tttcatcttc
     7081 ttgttcgtat ggaaaatcag tttcaattcg cgattaccgc tcatttccat gatgaaaact
     7141 tcgcttcgaa atccacactc gctcatgaaa cgatacggtt
//
LOCUS       DAOSOR010000286         7156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10490_length_7156_cov_5.166315, whole genome shotgun sequence.
ACCESSION   DAOSOR010000286 DAOSOR010000000
VERSION     DAOSOR010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7156
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10490_length_7156_cov_5.166315"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1356
                     /locus_tag="PK710_14895"
     CDS             <1..1356
                     /locus_tag="PK710_14895"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOT11064.1"
                     /translation="DGFRIPVRLSVAPIRDRNGKSTGTVAILRDTVAEREAAFAYRES
                     EQRYRAVFYNNPDAILVLDAKRNVVDANPAATGLTGLARDQLLGREMTELLEPSEMPS
                     MVEALSIMRQRGTMYRPLVRFRRTAEHTIPTEVHGTSLGPQLWQVLARNIESRLEAQE
                     KLRALNDQLEEKVAARTAQLKEAYAKIEHAGRMKDEFLANMSHELRTPLNSVLGLADA
                     LLEGVFGDVDEQQQQIVQTMRDSGHHLLDLINDILDLSKIEAGRLTLDLRPIRISEVC
                     QVSLRMIRPLALDKRIGSWMRVDPRCPVAVADERRLKQMLVNLLVNAVKFTPAGGQVG
                     LEAWMENDSVFFSVCDTGIGISPPDLRRIFEPFTQIDSSLARQHEGTGLGLALVRRLA
                     ELHQGHIDVVSEPGQGSRFTIVLPRVEPDVDVYSADDIEPVTLPRHPRTDGTCLDDED
                     K"
     gene            1353..2486
                     /locus_tag="PK710_14900"
     CDS             1353..2486
                     /locus_tag="PK710_14900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOT11065.1"
                     /translation="MTTDSPLLLIVDDDATARQVAQAFLSREGYTFRHAAGGEAALSS
                     VEEQEPDLILLDVMMPGIDGFEVCRLIRQRNTQAYLPIIMLTALDNPNEVAKGLDAGA
                     DDFITKPARRLELRARVRSMLRIRQQHMAIVQQANQLEELNNQREDLVRMVVHDLRSP
                     VTAVQLAASALLANEAVARADTDGDLTLIKEEARRVGNYLEEILLLARKEEGRLSLSF
                     SRVDLSELTRETIHALMPVAKARKQRIVLEPAPQGAAVILGDAALLRRVVDNLLTNGI
                     KFSPPESLIEVRVSVQGGQVALDVLDEGPGVPEHMRKRIFEKYEIVKVRAAGGPQTGL
                     GLAFSRTVVEAHGGYISCLPREGKGSQFHVTFIAAPTLNGDGS"
     gene            2808..4127
                     /locus_tag="PK710_14905"
     CDS             2808..4127
                     /locus_tag="PK710_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOT11066.1"
                     /translation="MSEKRVVILGAAGRDFHNFLVACKDDPNQRVVAFTATQIPDIDG
                     RRFPASLAGPRYPEGIPIEAEDQLENIIRDMKVDECVFAYSDVTHEYVMHLASRIQAA
                     GPSFRLMGPNETMLKSSKPVVSICAVRTGCGKSQTSRRVVKALRSAGKKVVSIRHPMP
                     YGDLAAQRVQRFATFEDMDKHNCTIEEREEYEPHVAAGAVIFSGVDYAAILAEAEKEA
                     DIIIWDGGNNDFSFYHPDLEIVVVDPLRAGHELAYHPGETVLRRAHVVVINKYKQAKP
                     EDIELLRKNIASVNPKATVINANSPVTLDNEAAVKGKRIIVVEDGPTLTHGGMAIGAG
                     WVAAQDLQCTIVSPRKYAVGTIKETYEKYSQTENVLPAMGYSKQQLADLNETIAKTVQ
                     AEKPDAVLSATPIDLGKLVTTTVPIVRASYELEEIGSPTLADLVTKL"
     gene            4322..5323
                     /locus_tag="PK710_14910"
     CDS             4322..5323
                     /locus_tag="PK710_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11067.1"
                     /translation="MVPFSEPKHGILHRFILLFCLGGGPAIAQVALSTAAFASPVHLT
                     LAPKSRAEELAQQARDALKASRYEEAISLMTRARDLEPGPRWTVELARAIAASGQLLR
                     ARQLLSKAIEKARPEQRYYLEAARDELEARIPTLRIQVSGPAPGVASVEIDGEKKEGS
                     VHQLDPGPHSVRVSAPGFLTAEEQTTLLEASPHELLVTLRLDSTPDPVPSVPVTSSSS
                     AWRRPVAVGSFVVAGAGVVTGALLWMKRNGKNREANDLFDQCDPRVCSKEERQRIANL
                     DDEAKNAAIIAGVSLGVAAIAAGTGAFLLWDESADTADQRALVVEPGLGSLLLRGTF"
     gene            5673..6902
                     /gene="rocD"
                     /locus_tag="PK710_14915"
     CDS             5673..6902
                     /gene="rocD"
                     /locus_tag="PK710_14915"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOT11068.1"
                     /translation="MTSRTQDVIERSEHWGAHNYHPLPVVLTKGQGAFVWDIEGKKYL
                     DMLSCYSALSHGHCHPRILAAAQKQMEQITLTSRAFHNVPYAEFCEKISRLSGLDMAL
                     PMNSGAEAVETALKAARAWGYRIKKVPQDKARIIAAKGNFHGRTIAIVSFSTEQAYRE
                     PFGPMAPGFDMVPFGDADALENAITPETVAFLVEPIQGEGGIIIPPDGYLKRVREICT
                     KHNVLMMLDEVQTGLARTGKMFAFEHEPGVKPDVLILGKALGGGLLPVSACVGTREVM
                     STFQPGEHGSTFGGMPLSMAVANEALDVLIQDELAERSARLGAEVVARLRQAKAPGVR
                     EIRGRGLLIGIEFEDFMGGAMNVAQALMHKGVLAKDTRVNTMRLAPALTIDESDLFEA
                     VDTVIATIRQVASTKSS"
BASE COUNT         1583 a         1989 c         2069 g         1515 t
ORIGIN      
        1 gatgggttcc gcattcccgt tcgcttgagt gtggcaccga tacgtgatcg caatggcaaa
       61 tcgaccggca ccgttgcaat cttgcgcgac acggtggcgg agcgagaggc ggcgtttgcc
      121 tatcgagaaa gcgaacaacg gtatcgagcg gtgttttaca acaacccgga tgcgatcctc
      181 gtgctcgatg ccaaacgcaa cgtggtggat gcgaaccctg ccgctacggg actgaccgga
      241 cttgctcgtg accagctcct tggccgcgaa atgacggaat tgctcgagcc ttccgagatg
      301 ccttccatgg tggaggcatt atcgatcatg cggcagcgag gaacgatgta tcggcctttg
      361 gtacggtttc gaagaacggc agaacatacg atcccgaccg aagtgcatgg cacatcgctt
      421 ggtccacagc tctggcaggt gctcgcgaga aacatcgagt cacggctcga agcgcaagaa
      481 aaattgcgcg cgctcaatga tcagttggaa gaaaaggtcg ctgcgagaac ggcgcaactc
      541 aaagaagctt atgcgaagat cgaacatgca ggacgcatga aggacgagtt tctcgccaac
      601 atgagtcatg agcttcggac ccctctcaat tccgtgcttg gacttgcgga tgccttgctc
      661 gaaggcgtct tcggagacgt cgatgagcag caacaacaaa tcgtgcaaac gatgcgtgac
      721 agcggacatc acttgctcga tctcatcaat gatattttgg atctttccaa aatcgaggcc
      781 ggacgattga ccctcgatct tcgaccgatt cgtatcagcg aagtgtgtca agtctcattg
      841 cgtatgatcc gtcccctcgc gttggataaa aggatcggtt cgtggatgcg cgtcgatcca
      901 cgttgccccg tggcggtggc ggatgaacgt cgtctcaagc aaatgctcgt caacttgctc
      961 gtgaacgcgg tgaagttcac gcccgccgga ggtcaggtgg gattggaagc ttggatggaa
     1021 aacgacagtg tttttttctc tgtctgcgat acgggcattg gaatttcacc accggattta
     1081 cgcagaattt ttgagccatt cacacaaatc gatagctctc tggcgcgaca gcatgaaggc
     1141 acggggcttg gtcttgcgct cgtgcgacgc ctcgctgaat tgcatcaggg gcacatcgat
     1201 gtggtttcgg agcccggcca gggaagccgt tttacgattg tgctgcctcg tgtggaaccg
     1261 gatgtcgatg tgtattcagc cgatgacatc gagcccgtga cgctgccacg tcatccacga
     1321 accgatggca cttgccttga cgatgaggac aaatgactac ggactctccg ctattgctta
     1381 tcgtcgacga tgatgcgact gctcgtcagg ttgcccaagc gtttctttcc cgggaaggct
     1441 acacgtttcg acacgccgcc ggcggcgaag ctgctctttc ctcggtcgag gagcaggaac
     1501 cggacctcat cttgcttgat gtcatgatgc caggaatcga tggcttcgaa gtatgccgcc
     1561 tcatccggca acgaaatacg caagcctatc tgcccattat catgctgacg gctttggata
     1621 atcccaatga agtagcgaaa ggattggatg cgggggctga tgacttcatc accaagccag
     1681 cgcgtcgact ggagcttcgc gcgcgcgtgc gttcgatgct tcggattcga caacaacaca
     1741 tggctatcgt tcaacaagcc aatcagctcg aagagttgaa caatcaacgc gaggacttgg
     1801 tgagaatggt tgtccatgac ttgcggtccc cggtgaccgc tgtccaactc gccgcctcgg
     1861 cgttgttggc caatgaggcc gtggcgcgag cggataccga tggggacttg accctcatca
     1921 aggaagaagc gcgacgagta ggaaactacc tcgaggagat tctgttgctc gcgcgcaaag
     1981 aagagggacg tctcagcctt tctttttctc gtgtcgatct atcggaactg acacgagaaa
     2041 ccattcatgc gctcatgccc gttgcgaagg ccagaaagca acggatcgtg ttggaacccg
     2101 caccgcaagg cgcggcggtc atcttgggcg acgccgcgtt gttgcgtcgt gtggtggata
     2161 acttgctcac caatggcatc aagttcagtc cgcccgagag tctgatcgag gtccgggtaa
     2221 gcgtgcaagg ggggcaggta gcgctcgacg tcctcgacga gggtcctggg gtaccggagc
     2281 atatgcgcaa acgaattttc gagaaatatg aaatcgtgaa agtgcgagcg gccgggggac
     2341 cccaaaccgg cttgggtctg gcgttcagcc gcaccgtggt ggaggcgcac ggaggctata
     2401 tctcctgctt gccccgggaa ggaaaaggaa gccagtttca cgttaccttc atcgccgccc
     2461 ccaccctcaa cggcgacgga tcatgatatt atctaataat ttcaacagat tatctcgatg
     2521 ccggcaaact agaaacgacg gtatttgctc aggtggcatt tcgtagtaag aggttgtgtg
     2581 caaaccggtg cgagcgtggc tttgccgcca tcatcctcgc tttctcgggt ggtgatcggc
     2641 cggttcgcaa tcatcgcttg gtcgcatccg acgcggccag gcatcaacgt ccacctttgt
     2701 cccatgggga cagcccgggc ttgaccgtcg gagtctcgac accggcagag tcgcctcggc
     2761 caccggtctc gcgtatggct cgcgagcaca cggaggagtg atcggtcatg agcgaaaagc
     2821 gtgtagtgat cctgggtgct gcagggcgtg acttccacaa ctttctggtt gcctgcaaag
     2881 acgatcccaa tcagcgggtg gtagctttca cggcgaccca gatcccggat atcgatggtc
     2941 ggaggtttcc cgcatccctt gcggggcctc gctatcccga gggtattccc atcgaggccg
     3001 aagaccagct cgaaaacatc atccgcgaca tgaaggtcga cgaatgcgta tttgcttata
     3061 gcgatgtgac ccatgagtac gtgatgcacc tagcaagccg tattcaagcg gctggtcctt
     3121 cgttccgtct gatgggaccg aatgaaacga tgctcaagtc ttccaaaccg gtcgtgagca
     3181 tttgcgccgt gcgcaccgga tgcggaaaga gccaaacgtc tcgtcgtgtg gtcaaagcat
     3241 tgcgaagtgc gggaaaaaag gtcgtatcga ttcgccatcc catgccttac ggcgatctgg
     3301 cggcgcaacg tgtgcagcgg tttgccacgt tcgaagacat ggacaagcac aactgcacca
     3361 tcgaggagcg cgaggagtac gagcctcatg tcgcggcggg tgccgtgatt ttctcaggag
     3421 tcgattatgc cgcgatcctc gcggaagccg agaaagaagc cgatatcatc atctgggacg
     3481 gcggcaacaa cgacttttcc ttctaccatc cggacctgga aatcgtcgtc gtggaccccc
     3541 ttcgcgcagg tcatgaactt gcctaccacc cgggagagac cgtgcttcga cgcgctcacg
     3601 tcgtcgtgat caacaaatac aaacaagcga agcccgaaga catcgagcta ttgcgcaaga
     3661 acatcgcttc ggtcaaccca aaggcgaccg tgatcaacgc caactcgccg gtcaccttgg
     3721 acaacgaggc cgcggtgaag ggcaagcgca tcatcgtggt cgaagacggt cccacgctca
     3781 cccatggcgg catggccatt ggcgccggct gggtggccgc ccaggatctg caatgcacca
     3841 tcgtatctcc gcgcaagtac gcggtgggca cgatcaagga aacctacgaa aaatacagtc
     3901 aaacagagaa tgtgctgccc gcgatgggat actccaaaca gcagcttgcc gacttgaacg
     3961 aaaccattgc caagacggtc caggccgaaa agcccgacgc cgtgctttcg gcaactccga
     4021 tcgaccttgg caaactcgtg accactaccg tgccgatcgt gcgggctagt tacgaactcg
     4081 aagaaatcgg ctcgcccacg ctcgcggatc tcgtcacgaa actctgatcc cttctccctt
     4141 atatttcctc acgcgccact gtcatgatct tgtgccatgg cttgatgcta acgaatgtat
     4201 cgccagggcg tcgatgcaac ccaatcgaaa accctcgatc gaaggaaatg caaaccgacg
     4261 aaaacatgga tctattggtc ttttcgtgtt ttcttagtgt ttcctacgac ccatgcgaac
     4321 gatggttccc ttctctgagc ccaagcacgg catccttcat cgattcatcc ttcttttttg
     4381 tcttggcggc ggaccggcta tcgcgcaggt tgcgctatcc actgcagctt tcgcgtcgcc
     4441 tgttcacctc accctggctc ccaaatccag ggcggaagaa cttgcacaac aagctcgtga
     4501 tgcattgaaa gcctctcgct acgaagaggc gatctcgcta atgacccgtg ccagagacct
     4561 cgaacctggt ccgcgatgga cggtcgaatt ggcgagagcg attgcggctt cgggccagtt
     4621 acttcgcgca cggcaactgc tctcgaaggc aatcgaaaaa gccaggccag agcagcgtta
     4681 ttatctcgaa gccgcacgcg acgaactcga ggctcgaatc cccacgcttc gaattcaagt
     4741 cagtggaccc gcccctggcg tggctagcgt cgaaatcgac ggcgaaaaga aggaaggcag
     4801 cgttcatcaa ctcgatcctg gtccgcattc cgtacgcgtt tccgcaccag gctttctcac
     4861 cgccgaagaa caaaccacat tgctcgaagc ctcgccccat gaacttctcg tcacgcttcg
     4921 cctcgactcg actccagatc cggtgccgtc cgtgccggtc acaagctctt cgagcgcgtg
     4981 gcgacggccc gtggccgttg gatcgtttgt cgtggcgggc gcgggtgtcg tgacgggcgc
     5041 gctgttgtgg atgaagcgca atggcaagaa tagggaagcc aatgacctat tcgatcaatg
     5101 cgaccctcgg gtgtgttcga aagaggagcg acaacggatc gcgaatttgg atgatgaagc
     5161 aaaaaatgcg gccatcatcg cgggtgtcag cctgggcgtg gcggcaatcg ctgccggcac
     5221 gggtgcgttt ctcttgtggg atgagtccgc ggacaccgcc gatcaacgcg ctctggtcgt
     5281 agagcctggt ctgggctccc tgctgttgcg cggaactttc tgaaacgagt tcccgggcct
     5341 gccctattca acggattcta taagcgaaac aacagcttag cagaaatctt cccgaccgtt
     5401 cgagcctcgc ttacgtttcc tattcgtcat gccgaattcg atgagcatcg tcgcacgtgt
     5461 tttctcattt cgcgacacac aaggctttgt ggatgttcca tcattcgaag ccggttgggg
     5521 ttgactattg aacaggcaaa gggcagcttc cgactggcga aagggaaggt cctggacgtt
     5581 tcgtcggacc cccgatgagc aagcccttga cgtatcgtcg ggcaaccatt cacgaccctt
     5641 ttggccgttt cacgccaaaa aaggagcagc gcatgacgag tcgaacgcaa gacgtcatcg
     5701 aacgatccga gcattgggga gcccataatt atcatccgtt gccggtggtt ctgacgaaag
     5761 ggcaaggggc tttcgtttgg gatatcgagg gcaaaaagta cttggacatg ctgtcgtgct
     5821 actcggcatt gagtcacggg cactgccacc cgcgtatcct tgccgcggcg caaaaacaga
     5881 tggagcagat cacgctcaca tcgcgtgcct ttcacaacgt tccctacgcc gagttttgcg
     5941 aaaagatatc gagactgtcg gggctcgaca tggcgcttcc gatgaactcg ggagcggagg
     6001 ccgtggagac ggcgttgaaa gccgcgcgtg catggggcta tcgcatcaag aaagtccctc
     6061 aggacaaggc tcgcatcatc gccgccaaag gaaattttca cggtcgcacg atcgccatcg
     6121 tctcattttc caccgaacaa gcgtatcgcg aaccattcgg cccgatggcc cccggattcg
     6181 acatggttcc ctttggcgat gccgatgctt tggaaaatgc aatcacccct gagaccgtcg
     6241 ccttcttggt cgaaccgatt caaggggaag gaggcatcat cattccgccg gacggctact
     6301 tgaaacgcgt gcgagaaatc tgcaccaagc acaatgtgtt gatgatgctc gacgaagtgc
     6361 agacggggtt ggcgcgcacc ggcaagatgt tcgcgttcga gcatgaaccg ggcgtcaagc
     6421 cagacgtact catcttggga aaagcgttgg gtggaggact tcttccggtg agtgcttgcg
     6481 tggggacacg cgaggtgatg tcgacgttcc agccagggga gcatggttcg acctttggag
     6541 gcatgccgct atcgatggcg gtcgccaacg aagccctcga tgtattgatt caagacgagc
     6601 ttgcggaacg ctcggcgcgt ttgggtgcgg aagtcgtcgc gcgacttcgt caggccaagg
     6661 ctccgggagt gcgagagatt cgtgggcgtg gattgctcat cggcatcgag tttgaggact
     6721 tcatgggcgg tgccatgaac gtagcgcaag cgttgatgca caaaggagtg cttgccaaag
     6781 acactcgtgt gaacaccatg cgtttggccc ccgctctcac gattgacgaa agcgatcttt
     6841 tcgaagccgt ggatacggta atcgcaacga ttcggcaggt ggcgtcgacg aaatcatcct
     6901 gagccacgcg tccacactcg cgcgcccttc tctggcaaat cagtattatc taataattac
     6961 aacaggttaa cccgatttcg cgaggcatgt ctcaagcaag atccgccaag agtttggggg
     7021 cttgcacgcg cagcatgtac cggcctttgc cgatatttcc cgtgggttcg agggccaggg
     7081 ccacgaagta ttcgtgggta agaacccgga taagggtgat gacgcgctcc gattgtacgg
     7141 aaagctcccg ggtttc
//
LOCUS       DAOSOR010000287         7131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10540_length_7131_cov_3.810345, whole genome shotgun sequence.
ACCESSION   DAOSOR010000287 DAOSOR010000000
VERSION     DAOSOR010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10540_length_7131_cov_3.810345"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="PK710_14920"
     CDS             complement(<1..1220)
                     /locus_tag="PK710_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11069.1"
                     /translation="MNRFGSSVFSVLKRQLLLMFCATWLSCSADPLTELVVVINSDME
                     TPDEFDGFRVAVTDHEGALSTERYYFVGKEPGKVVLPADFGLVPKGGDASRKVAVEVW
                     ATLAGADLFSNRALTGFVKGKVLRLDMFLASRCLTEAKACKPNETCRVEGCVPEEIDP
                     GSLPEYTPGSQPPATEWIAAIHSSRSSRWWNVALDSAGNSYAAGMFAGEFKAQGVTHT
                     ASSLYSAMIASYSPTGKMRWFKALGGNTLSWASSVEVGADDRVCVVGWFDGSVQIESK
                     TLDSGSGQDSFVACYDQKGDLLWVTSFGSHKNVQAKDIAVAPNGDVAVVGVYNAPIVF
                     EGGVTTPTSVNTTDDGFMARFDSSGKIKSYRVLGGTGEADVVWNVAFDSQSNLLMVGT
                     LTKGDVDLGGGVET"
     gene            complement(1404..2525)
                     /locus_tag="PK710_14925"
     CDS             complement(1404..2525)
                     /locus_tag="PK710_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11070.1"
                     /translation="MAVRRNNFVIAGLGSSGTLFLAQRMNQSQIWQVVHTGVRHRVDL
                     DEASSKPEHSIPMTLPSLSEAQAGYDAHPRNFGDINGVMLFHLLELQVGRKAVIIRNP
                     RDTLLSWYCHAKGALPGAFFPIYASGYFALEECLTAGLPCIRFERMVRDADYLRRVFR
                     YFGILDVTPTPATLGSKINSKRSVFCPSYEAIDRVTRERFDRAVGGFASKYYGDGPLL
                     HDDDFKGVGEQGLGDPDGPEVEESVFAKQDLNPGSSFEKPDSLATEALTRLASRLTLN
                     PPGATGAEPRLVHAQVFEDRLVLHISSDAQESDRIVIERRKDGVPYYLRTRYLHLSYV
                     GSTSSSGLQATFDVLVRQLGDTRFEQLMVEVEPSGPHGG"
     gene            complement(2569..3540)
                     /locus_tag="PK710_14930"
     CDS             complement(2569..3540)
                     /locus_tag="PK710_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOT11071.1"
                     /translation="MIHRRSFLVGFGALGVLGSCKKQGQAAMPRVAVIPKGTTHEFWK
                     TVHAGVKQAAQEEGIDAVWKGPLKEDDLKAQIELVQSFVAQRVAAIVLAPLSDKALLG
                     PVKAAKSSGIPVVIFDSALQGEEHDALVATDNLAAGRLAAEEMQKVLGGKGKVVVLRY
                     LEGSASTMEREKGFLDTIRKVTGIEVVSDNQYGGATTESAFSTSETLLSARQAATGGV
                     DGIFAPNESTTFGMLLALRKANLAGKIKFIGFDASDKLVEGVRQGHLHALVLQNPRKI
                     GYMALKRAAQLLRKEPVERRFDTGAQLVTRDNLDDDGVRELLRAPEP"
     gene            complement(3629..5293)
                     /locus_tag="PK710_14935"
     CDS             complement(3629..5293)
                     /locus_tag="PK710_14935"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOT11072.1"
                     /translation="MGERAFVGVDVGTASVRAGVVDRNGRMLGVGVEAITVSRPSEDF
                     VEQSSEEIWRACGLAIRKALQEAGVEPGAIAGLGFDATCSLVVVDAQGRPLSVNSDEA
                     AERNVVVWMDHRATEQASRINATKHEVLRYVGGGVSPEMQTPKLLWIKENLPETWGRA
                     ARFYDLPDYLVVRATGVDVRSECTTTCKWTYLAHENRWDEGFFQQVGLGDLAADGFEK
                     IGSKVAPVGSRAGELTELSARELGLVAGTAVAVALIDAHAGGVGVLGFPNENGDLSAD
                     LLEQRLALIGGTSTCHMVVSKEPRFVPGVWGPYFSAMVPGLWLSEGGQSATGALIDHT
                     IQTHCRGRECMERAKNEGISVYSLLNGLLETMSGAYPFPAALTHELHVLPDHHGNRSP
                     WADASLRGMVSGLRLTDSLESLALLYLATIQALAHGTKHILDVMNAHGHRITTLVATG
                     GDSKNPLFLREHADVTGMRIVLPREREAVLLGSAMLGAVASSHFPSLLEAMRKMGGVE
                     REIVPAGGALARYHEAKHRVFLRMYEDQRAYREAMAQAAFALGSTG"
     gene            complement(5315..6826)
                     /locus_tag="PK710_14940"
     CDS             complement(5315..6826)
                     /locus_tag="PK710_14940"
                     /inference="COORDINATES: protein motif:HMM:NF014948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11073.1"
                     /translation="MTSKIALFWPGDGRPIPNELARPSMQRATEQLERALRKLGKEPY
                     LIEGFLSKPHESIEKLGPVHDPMIGVCVHWIYGPHTTDGVVGKDNPLLLASNFSGQWP
                     GLVGLLNTAACLEMLGRPYSRVWTDADDWSRDDVFMSRLDAWCSTGRISYEEQGFSYH
                     AVISSEASRLAKRVAEQIRARRVLVMMLGDTSMGMINGYFGPRTLVNHGFSEHKIDQA
                     WIVDRGRAVDSRRIDDAFSFVLDRGVTFHWREKGAEDFDEAATKEQIRDYLSVLDLVR
                     EYKADCVGWQYQLGLLPLRPPSDFAEGLLNSVCRPESNGDTIVCATEADQGNVVPMEL
                     MKRLLKAKGLHQAVMFHDVRWGGEHDGRFVWVLLNSGSTGAYAFNHDPDTLRGVHSYR
                     QPSLYFPHAGGTFTGESMPGAITWARAFVRGEQTWMDVGRGEVLALPEQTKEAWWQGT
                     NREWPFMAADLGMSRDALMAHYMSNHIAVAYGDVFEEMVALSQELGFRVRVWG"
BASE COUNT         1572 a         2352 c         1801 g         1406 t
ORIGIN      
        1 aaggtttcca ccccaccccc gagatccaca tcgcctttgg tcaacgtgcc aaccatgagc
       61 aagttggatt ggctgtcgaa agcaacattc cagaccacat cggcttcccc cgttccccca
      121 agcacccggt aggatttgat cttgcccgac gaatcgaatc gagccatgaa accgtcgtcg
      181 gtggtgttga cgctcgtggg agtggtcacg ccaccctcaa aaacgatggg agcgttgtac
      241 acgccgacga cggccacatc gccgttgggt gccacggcga tatcctttgc ctgaacgttt
      301 ttgtggcttc caaaagacgt aacccatagc aggtcaccct tttggtcata acaagccacg
      361 aaactgtcct gtccacttcc tgaatcgagg gttttcgact cgatctgaac cgaaccatcg
      421 aaccagccga ccacacatac ccgatcgtcg gcgccgacct ccaccgacga agcccacgac
      481 aaggtattgc cgccaagtgc tttgaaccag cgcattttcc cggttgggga atagcttgcg
      541 atcatggcgg aatacaggct ggaagcggta tgggtcactc cctgggcttt gaattcaccg
      601 gcgaacatgc cagcggcgta actatttccg gccgagtcca gggccacgtt ccaccaacgg
      661 ctcgaacggg agctgtggat ggcggctatc cactcggtgg caggaggctg cgatcctggc
      721 gtgtattcgg gcaggctccc cggatcgatc tcctcgggca cacatccttc gacacggcag
      781 gtttcgttgg gtttacaagc cttggcttcc gtgagacagc ggctcgccaa aaacatatcc
      841 agccgcaata ccttgccttt gacgaagccc gtcaaggcgc gattggaaaa taggtcggct
      901 cccgcaagcg tggcccacac ttccacagcg acttttctcg atgcatctcc gcccttggga
      961 accaatccga agtccgcggg aagcaccact tttcccggct ctttccccac gaaataatag
     1021 cgctccgtcg agagcgcccc ctcatgatcc gtcaccgcca cgcgaaaacc atcgaattcg
     1081 tcgggagtct ccatgtccga gttgatgacc accaccaatt cggtaagagg atccgcggaa
     1141 catgagagcc atgtcgcgca aaacatcagc aaaagctgac gtttcaggac tgaaaaaacg
     1201 ctcgagccga atcgattcat cgcaaacgag gttgtaccac aaaagccaaa ggaaaaacgg
     1261 ctacgaacca tttcttgcct catcacgcga tggctcacct ggattcgtga aaaagctggt
     1321 tccccagcat ccttgccatg gatcctcatg gacacacccg caccacggaa gcacgtttcg
     1381 agccaactag ccctggccgc ccctcacccc ccgtgcgggc ccgacggctc cacttcaacc
     1441 ataagctgct cgaaacgcgt atccccaagt tgtcggacaa gcacatcaaa cgttgcctgt
     1501 aatcccgaag acgatgtcga tccaacgtaa ctcaagtgta agtaacgcgt gcggagataa
     1561 tagggcaccc catctttccg acgttcgatc acgattcgat cgctttcctg cgcatccgac
     1621 gaaatgtgca acaccagccg atcctcgaaa acctgggcgt gaacaaggcg tggctctgcc
     1681 ccggttgcgc ccggtggatt caatgtcaaa cgagatgcca gacgcgtcag cgcttcggta
     1741 gcaagcgaat caggcttttc aaaagacgaa cccgggttaa ggtcctgctt tgcgaaaacg
     1801 ctctcctcca cctcgggccc gtcaggatca cccaatccct gctcgcccac acccttgaaa
     1861 tcatcgtcgt gaagcaaggg accatcccca taatacttgg aagcgaagcc tcccaccgcg
     1921 cgatcgaacc gctcccgggt cacgcgatcg atggcttcat aagaggggca aaacaccgaa
     1981 cgtttcgagt tgatcttgct gccgagcgtg gcaggagtcg gtgtgacgtc cagaattcca
     2041 aaatacctga aaactcttcg taaataatcg gcatcgcgca ccattcgctc gaagcgaatg
     2101 caaggcaatc ccgccgtaag acactcctcc aaggcgaagt atccgctcgc atagatagga
     2161 aaaaacgcgc ccggaagcgc gcccttcgca tgacaatacc acgacaagag cgtgtcccga
     2221 ggattgcgga tgatcaccgc tttgcgccct acctgaagct ccagcaagtg aaataacatc
     2281 accccgttga tatcgccgaa atttcgagga tgcgcatcgt aacccgcctg cgcctcggac
     2341 aacgacggca acgtcatcgg gatcgaatgc tccggcttcg acgatgcctc atccaggtcc
     2401 acccgatggc ggacgcccgt atgaaccact tgccatattt ggctttggtt catgcgctgc
     2461 gccagaaaaa gcgtgccgct actgccaagc cccgcaatca cgaaattgtt tcgtcgaaca
     2521 gccataaaaa cctccgccat cgcaagcgta atcgctgagc tggatgcctc acggctccgg
     2581 agcgcgaagc agctcccgaa ccccatcgtc atccagattg tcgcgcgtca cgagctgcgc
     2641 cccagtgtca aatcgtcgct ccaccggttc cttgcgcagg agctgagccg cccgcttcag
     2701 agccatatag ccgattttcc ttgggttttg gagaacgagg gcatgcaaat gaccttgcct
     2761 caccccctcc acgagcttgt ccgacgcatc gaagccgatg aacttgattt ttcccgcgag
     2821 attcgccttg cgcagggcca agagcatgcc aaacgttgtg gattcattcg gagcaaagat
     2881 gccatccacc ccgcccgtgg ccgcctgacg cgccgaaagc agcgtctcac tggtcgaaaa
     2941 ggcactctct gtggtggctc ccccgtattg gttgtcgctc accacctcga ttcccgtaac
     3001 tttgcgaatt gtatcaagaa atcccttttc ccgctccatc gtgctcgcgg aaccctccag
     3061 gtatcgcagc accacgacct tccccttgcc gcccaatacc ttctgcattt cctccgccgc
     3121 aagccggccc gccgccaggt tgtccgtcgc caccagcgca tcatgctcct cgccttgcag
     3181 cgccgagtcg aaaatcacca ccggaatccc tgacgacttc gccgccttga ccggacccaa
     3241 caacgcctta tcgctcaatg gcgccagcac gatcgccgct accctctgcg ccacgaaact
     3301 ctgcaccaat tctatctgcg ctttcagatc atcttccttg agcggccctt tccataccgc
     3361 gtcgatcccc tcctcttgcg ccgcttgctt gacccccgca tgcacggtct tccaaaactc
     3421 atgggtcgtg cctttcggga tcaccgccac ccgaggcatc gccgcctgcc cttgcttttt
     3481 acaagaacca aggaccccca acgctccaaa acccaccaaa aacgaccgtc gatgaatcat
     3541 gctcttccct cacgaccccg tgccaccgcc tctggggtga caacgatgac aatccatgga
     3601 aacgatagag tcaacaccca cttccacgct atcccgtgga tcccaacgcg aaggcggcct
     3661 gggccatggc ctcccggtac gccctctgat cctcgtacat cctcaaaaat acgcggtgtt
     3721 tcgcctcatg gtagcgcgcc agggcgccgc ccgcaggaac gatctctcgc tcgactcccc
     3781 ccatttttcg catcgcttcg agcaaggagg gaaaatgcga cgacgccacg gctcctagca
     3841 tcgccgagcc caaaagtacc gcctcccttt cgcggggcaa gacgatccgc atgcccgtca
     3901 catccgcatg ttcgcgtaaa aacaagggat ttttcgaatc cccgccggtg gctaccagcg
     3961 tcgttatccg atgaccatgg gcattcatca catccaaaat atgtttcgta ccgtgagcaa
     4021 gcgcctggat cgtcgccaga taaagcaacg ccaagctctc caagctgtcg gtcaatcgca
     4081 acccactcac catcccgcgc aaggaggcgt cggcccaagg cgagcgattg ccgtggtggt
     4141 cgggcaatac atgcagttca tgggtcagcg cggcggggaa aggataagct ccgctcatcg
     4201 tctccaagag cccattgagc aaggaataca cggatattcc ttcatttttc gcgcgttcca
     4261 tgcactcccg cccgcgacaa tgggtctgaa tcgtatggtc gatgagcgca ccggtagctg
     4321 attgcccccc ttcactcagc cataggcctg gcaccatggc agaaaagtaa gggccccaca
     4381 cgccgggcac gaaacgcggt tccttcgata ccaccatgtg gcacgtcgag gttccgccga
     4441 tcagcgccag acgttgctcc aacagatcag cactcaagtc accgttttca ttaggaaaac
     4501 caagaactcc caccccgccc gcgtgggcat cgatgagagc gaccgccacg gcggtgccgg
     4561 ccaccagccc cagttcccgg gccgacagct ccgtcaactc gcccgcgcga ctccccacgg
     4621 gtgccacttt tgaaccgatt ttttcaaaac cgtcggcggc caggtcgccg agccccacct
     4681 gctgaaaaaa tccttcatcc cagcggtttt catgcgcaag gtaggtccac ttgcacgtcg
     4741 tggtgcattc cgaacgcacg tccacaccgg tggcgcggac cacgagatag tccggtagat
     4801 cgtaaaaccg ggcggcacgg ccccacgttt ccggcaagtt ctccttgatc cacagcagtt
     4861 tgggagtctg catttccgga gataccccgc caccgacata acgcaacacc tcgtgtttcg
     4921 tggcattgat ccgcgaggct tgctccgtcg cccgatgatc catccacacc accacgtttc
     4981 gctcggcggc ttcatcggaa ttcacgctca aaggacgccc ctgcgcatcg acgaccacca
     5041 gggagcacgt ggcatcgaag ccaaggcccg cgatggcgcc cggctccacg ccagcttcct
     5101 gcaacgcttt acgaatcgca agcccgcacg cacgccatat ttcctcggag gattgctcca
     5161 cgaagtcctc cgaaggccgg gatacggtaa tcgcctcgac ccccacaccg agcatgcggc
     5221 catttcgatc cacgactccg gcccgcacac tcgctgttcc cacatcgacg ccgacaaacg
     5281 ctcgttcgcc catggtgtgt tctcctcgga agcctcaacc ccagacacga acccgaaatc
     5341 caagctcttg cgacaacgca accatctctt cgaacacgtc gccgtaggcc accgcaatat
     5401 ggttggacat atagtgggcc atcagggcat cccgagacat gccgagatcc gcggccatga
     5461 aaggccattc ccggttggtc ccttgccacc aggcttcctt cgtttgctca ggcaaggcca
     5521 gcacttcgcc ccttcctaca tccatccagg tctgttcacc acgcacgaaa gctcgtgccc
     5581 acgtgatggc tcctggcata ctctcgcccg tgaaagtccc acccgcatgg gggaaataca
     5641 gcgagggctg gcgataggaa tgcacaccgc gcaacgtatc cgggtcgtgg ttgaacgcat
     5701 aggcgcccgt cgacccggag ttcaacagca cccagacgaa gcgtccgtcg tgctcccctc
     5761 cccagcgaac gtcgtggaac atgacggcct gatgcaggcc tttggctttc aataagcgct
     5821 tcatcaactc catcggcacg acgttcccct ggtcggcctc ggtagcgcaa actattgtat
     5881 ctccattgga ttccggccgg cagacagaat tgagcaggcc ttccgcgaag tccgaaggcg
     5941 ggcgcaacgg cagcaatccc aattggtact gccatcccac acaatcggcc ttgtattccc
     6001 gcacgaggtc gagcaccgaa aggtagtccc gaatctgctc tttcgtcgca gcttcgtcga
     6061 aatcctcggc tcctttttcc cgccaatgaa aggtcacgcc gcggtccaga acaaacgaaa
     6121 atgcgtcgtc gatgcgtcgg ctatccaccg cgcgaccgcg gtcgacaatc caggcctgat
     6181 cgattttatg ttccgaaaac ccatggttca cgagggttcg cgggccaaag taaccgttga
     6241 tcatgcccat cgacgtatct ccgagcatca tgacgagcac ccttcgagcg cggatttgct
     6301 cggcaactcg tttcgccagc cgcgatgcct cggaagaaat caccgcatgg taggaaaaac
     6361 cttgttcttc ataggaaatg cgtcctgtcg aacaccaggc atccagacga ctcatgaaaa
     6421 cgtcatcgcg actccaatcg tcggcatccg tccagacgcg ggaatagggt cgtccaagca
     6481 tttctagaca ggcagcggta ttgagaaggc ccaccaatcc tggccattga cccgagaagt
     6541 tgctcgcgag aagaagtgga ttgtcctttc cgacgacgcc atcggtggta tgcgggccgt
     6601 aaatccaatg gacgcaaacg ccgatcatgg gatcgtggac aggcccgagt ttctcgatgg
     6661 attcgtgggg tttggacagg aatccctcga tgagataggg ctctttgcca agcttgcgca
     6721 aggcgcgttc gagctgctcg gtggctcgtt gcatgctcgg acgagccaat tcgttgggaa
     6781 tgggacgacc atcgccgggc cagaacaatg cgattttgct tgtcatgagg ggggttagag
     6841 cattgtggca gcggcgatgc aaggggaggc gacacggcgg tgacgggttg tgtctttcgg
     6901 cgttggtgtg ggccctatcc gcggcgttgt ttccgccccc ccatcccctc ccttcccccc
     6961 acacggtgtg ccccccatcc ctggcccttc cccccacagt gtgggcagaa gggtgacaga
     7021 acatctaatt cgtattccta gtttctagtt tcaaaatatt ttagctaaat aattaaccac
     7081 tgtctctatt cccctccacc ccgattcggg ggggagggca agggtggggg g
//
LOCUS       DAOSOR010000288         7110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10581_length_7110_cov_4.198866, whole genome shotgun sequence.
ACCESSION   DAOSOR010000288 DAOSOR010000000
VERSION     DAOSOR010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7110
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10581_length_7110_cov_4.198866"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PK710_14945"
     CDS             <1..956
                     /locus_tag="PK710_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11074.1"
                     /translation="GGATGGSGGATGGSGGATGGSGGATGGSGGATGGSGGATGGSGG
                     ATGGSGGATGGSGGATGGTGGGSSNPEFIIGVDGIAAFDALSSADKDALKNTKVFFQH
                     MSVGENLLMGYNNWSPPWKGGTASLGFVFTNVNNASSYGSVKLGDKAFGYNGNPFNKI
                     NGFRQHILDMGIGNAVQVAGFKFCYNDLTVDITSTGKDVIDAYKSAYHDIENGTIGTS
                     FFHVTTPLQPANQWHTVENNKLRGTFAEFLRTNYAGGRHVVFDLQQIESTDANGSTCS
                     QSGVPVLCAAWAADNDGHLNDTGATRAAKAFLYALHVARGL"
     gene            complement(1133..1747)
                     /locus_tag="PK710_14950"
     CDS             complement(1133..1747)
                     /locus_tag="PK710_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11075.1"
                     /translation="MKRPNASQPVVITNESCFSNGLATWDLASDDSVLSEQRQRGPHR
                     SDFFERHISSKIDPAVQDPVAVVAAHRLNGEPILWWTTDHVDAVVDERFSGDLTVVDV
                     PRLRPGERRRLGQLSVEARVLAPRDVLAFLLRADIVRTYWHIASRVCLLRETVGADGY
                     QGELCGEHRYFTNTNHRAAFQFRFEINGMGELFVTGLETQGDVP"
     gene            complement(1845..2045)
                     /locus_tag="PK710_14955"
     CDS             complement(1845..2045)
                     /locus_tag="PK710_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11076.1"
                     /translation="MRPLQDDSVVEGIPGTHEKKPTRALWTGFCGDRASGPVAKDVIF
                     PRIPGGLIQTDAMRVRHAPQGL"
     gene            complement(2026..2151)
                     /locus_tag="PK710_14960"
     CDS             complement(2026..2151)
                     /locus_tag="PK710_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11077.1"
                     /translation="MSRPQRHSCVGLLRRSALRFLEKSLDFSLFEDSIGHAPTPG"
     gene            complement(2164..2370)
                     /locus_tag="PK710_14965"
     CDS             complement(2164..2370)
                     /locus_tag="PK710_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11078.1"
                     /translation="MADPARTPARGMARESFAGHDANAVAARPDRPERRGVEARVPKP
                     NEQHPYRQDLADASHRLIRERSQR"
     gene            2577..4085
                     /locus_tag="PK710_14970"
     CDS             2577..4085
                     /locus_tag="PK710_14970"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOT11079.1"
                     /translation="MNGCLFPDAPHEKLLRAGGKAAQLGRLAQAGMQVPAFFCLDQDA
                     LRGFLEHNHLSPHPQQRRDLDDFEKEVEAAFLSAAFPPELEQALVEILGSPPFSTHDL
                     AVRSSGLDEDSVEHSFAGQYATFLHQRGTKAVLGAIRRCWASAWSARVVSYRTMRGIG
                     NDNVGMGVVVQVMVNADRAGVAFSRHPLEPLDRKHLLISSVFGLGEGLVSGALDADHF
                     EIDRESFTTTSEVIAHKREALRADDEGGIRSYPLPESQRTEPSLSPEQRKQVARLCLR
                     CETRMGSPQDIEWAFENDSLFLLQSRPITTLPPDAFFDPAVVGREATLWDNSNIVESY
                     CGVTTPLTFSFASSAYRQVYIQFCEVMGVPPQEVETREAMFRNMLGLLRGRVYYNLIN
                     WYRLLMLFPGVADNNAFMETMMGVKDSMNPELAGMFEFMKHPPHYSTLRRAKLAGITL
                     VRFARIDSIVREFQEYFRDVYDQARATPIQDMGLVALASHYQHLEDKLLRRW"
     gene            4113..5294
                     /locus_tag="PK710_14975"
     CDS             4113..5294
                     /locus_tag="PK710_14975"
                     /inference="COORDINATES: protein motif:HMM:NF012609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HOT11080.1"
                     /translation="MVSFGLLKKLTDQWLGDSPEINNLHNDLLCGEGDLESTEPTKSL
                     MRIARRVDEGPTETRSLLLENPASEVWSQLLQGKAPQLRKDFEDFLDRYGFRCVNELK
                     LEEPDLHDDPSFAVQAVASYVRMKAYSIEAMEEREQSIRNQAEIAVRSRLGGPRRLAY
                     FWVVQQARKAVRHRENLRFARTKVFGIVRHLFRAMGDRFVRLGVLQDPHDIFYLTVDE
                     IFGFIEGRPSSLAFLPLVEARKREFAAYRQGPGLPDRFLTYGAVGASLPYPQVLHATD
                     LLRGKNQSQDPSLLTGIPCCPGVVEGTVRVVREMKDAEGLHGEILVTERTDPGWVPLY
                     PSCSALLIERGSLLSHSAVVARELGLPAVVGISGGLMQKLKTGDRVRVDGAAGTVTLI
                     S"
     gene            5513..6538
                     /locus_tag="PK710_14980"
     CDS             5513..6538
                     /locus_tag="PK710_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11081.1"
                     /translation="MTALHIRCLSESQPSEFVTSFCEIPYTLRRDPFRVGLLPPEAEA
                     LLLTLPLPNLREFWIATRQQRIVGRIGASLSADGTQGLVGFFEVDLDDETAARQLLET
                     AEAWLRCRGIRKVFGPVHINTWFPYRFRVEAHDEGAYSWEPVQPPEYPEQFQAFGFRV
                     DQVYHSDELVDLEAIRKGFEPHYRRTLQAGYTYRPIDTVELERELPVLYRLSLSGFSQ
                     NYLFEPISEPVFRALYVAQAAKRKPLGCHFVCHPTRGEVGFTVGFVDEAEQFVLKTLV
                     VLPEVQSQGLSMGLLFEAFGSGVRAGHPSAIAALVQAGNRSEASSRRTPIGWRHTYAL
                     FRKDLGE"
     gene            complement(6572..>7110)
                     /locus_tag="PK710_14985"
     CDS             complement(6572..>7110)
                     /locus_tag="PK710_14985"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOT11082.1"
                     /translation="KQEMGPLWEAILQYNAKQFPALFTPPRLAYLSRAIQRIGPLWRE
                     IEAAPRTLIHNDFNLRNICLRRTGSGFRLCAYDWELATMHLPQRDICEFLSFVLPPGT
                     SREGWLEYVETYRRALEKHAQRSLDAATFVRGFDLASFDYAIDRLGMLTIAQGVNVYE
                     YLPRVLHSHFEYLDGCSL"
BASE COUNT         1523 a         2071 c         1982 g         1534 t
ORIGIN      
        1 cgggtggtgc taccggcggc tcgggtggtg ctaccggcgg ctcgggtggt gctaccggag
       61 gctcaggcgg tgccaccggc ggctcgggtg gtgcgactgg cggttcgggc ggtgcaaccg
      121 gaggttcggg tggtgccact ggcggctcgg gtggtgctac cggcggctca ggtggcgcca
      181 ctggcggaac gggcggaggc agcagtaatc ctgaattcat catcggcgtc gatggtattg
      241 cagcctttga cgcactgtcc tctgccgaca aagatgcatt gaaaaatacc aaagtgtttt
      301 ttcagcacat gtccgtcggt gaaaacctgc tcatgggcta caacaattgg tctcccccat
      361 ggaaaggcgg cacggcctcc ttgggattcg ttttcacgaa cgtaaacaac gcgtccagct
      421 acggcagcgt caagctgggg gataaagcct ttggttataa cggcaatcca ttcaacaaga
      481 tcaatggatt tcgacagcat atccttgata tgggaattgg aaatgccgtg caggtggcgg
      541 ggttcaagtt ctgttataat gacttgacgg tggacatcac ctccacgggc aaggatgtca
      601 tcgatgcgta taaatccgct tatcatgata ttgaaaatgg gacgataggc acgtcatttt
      661 tccacgttac gacgccactg cagccggcca accagtggca tacggtagag aataacaaac
      721 tccgcggaac gttcgccgag tttctgcgaa ccaattacgc gggaggaagg catgtggtat
      781 tcgacttgca gcagatcgag tcgacggacg ccaacggctc gacatgcagc caaagtgggg
      841 ttcccgtgct ttgtgcggca tgggcagcgg acaacgacgg gcatctcaat gacacaggag
      901 cgacacgagc ggcgaaagcg tttttgtacg cattgcatgt ggcgcgagga ttgtaagccg
      961 catctgcaag ctgattcgat gctgtagagc ctggtatccc aatccaagca gaagccgtat
     1021 tgactaagcc gtacaatcga ctcccacaat cccacgcccc cgcaaaaaca gccattcggc
     1081 gattggactt ctcgaacgct agaccggagt caagcgtcga actcacccgt gattacggaa
     1141 catcgccttg tgtctcgagc cccgtgacga acagctctcc catcccattg atttcgaatc
     1201 gaaattgaaa agcggcgcga tggtttgtat tcgtgaaata acgatgctcg ccacataact
     1261 ccccttggta accatcagca cccaccgtct cgcgaagcaa acacacccgg cttgctatat
     1321 gccaataggt tcggacgatg tccgcgcgaa gcaaaaacgc caacacatcc ctgggagcca
     1381 aaactcgtgc ttccaccgac aattgtccga gccggcgacg ctcccccgga cgcaatcggg
     1441 gcacatcgac gactgtcagg tctccggaaa agcgttcgtc cacaaccgca tcgacatgat
     1501 ccgtcgtcca ccataggata ggctcaccgt tcagtcggtg cgcggcgacg acagcgaccg
     1561 ggtcttgcac ggcaggatcg attttgctcg aaatatggcg ctcgaaaaag tcacttcgat
     1621 ggggaccgcg ttgacgctgt tcgctcaata cgctgtcatc gctcgccaag tcccacgtgg
     1681 caagcccgtt ggaaaaacaa gattcatttg taattacaac aggttgcgat gcatttgggc
     1741 gcttcatggg gttgtccgtc gaggaaaggg tggtgggggt ggaaaaggcc gctggggaag
     1801 tcggcgtcgt acagcccatg agccatagcg cgatggctat tcgtttacaa accttgggga
     1861 gcatgacgaa ccctcatggc atcggtttgg atgagtccac cgggaattct cggaaaaatg
     1921 acgtctttgg cgacaggacc ggatgcacga tcaccgcaaa aacctgtcca caaagctcgg
     1981 gttggctttt tttcgtgtgt cccaggtatc ccttccacga cactatcatc ctggagtggg
     2041 cgcatgaccg atgctatcct cgaacaggct gaagtccaac gatttctcga gaaaacgcaa
     2101 tgcagaacgg cggagcagac ccacgcacga atggcgttga ggacggctca tgacgacgca
     2161 tcctcaccgt tgcgagcgct cgcgaatcaa gcgatgactg gcatccgcca ggtcttgacg
     2221 gtacggatgc tgttcgttgg gcttggggac ccgggcttct acccctcggc gctcgggacg
     2281 atcgggccgc gcggccacgg cgttggcatc atgtcccgca aacgattccc gggccattcc
     2341 ccgggccgga gttcgagctg ggtcggccat cgtggagtcg acgccatcgc gcctccaatg
     2401 cccttgtaac tcgcggtgta tacgttacgc tgctgcccaa tgccgtcgcg ctcgcaaccc
     2461 aatcgcccct ttttccccat tgttcgtgcg attgcgctcc acgagccggt agcaagcctg
     2521 tcgtttgccg cccacccctg ggtgcttact tttggacact tcataggatg accctcatga
     2581 acggctgcct attccctgat gccccccacg aaaaactgct gcgggcaggc ggcaaagccg
     2641 cccagctagg tcgtctggcc caggccggca tgcaagttcc cgccttcttt tgcctcgacc
     2701 aggacgctct ccgcggtttt ctcgaacaca accacctcag cccccatcca cagcagcgcc
     2761 gcgatctcga tgatttcgaa aaagaagtgg aagcggcttt cctttccgcc gcatttccgc
     2821 cggaactcga acaggccctt gtcgagattc tcggctcgcc gccgttttcc actcacgacc
     2881 tggctgtccg ttccagcggt ctggacgagg actccgtgga gcattctttc gcgggccaat
     2941 acgccacatt tcttcatcaa cggggaacca aggcggttct tggtgccatc cgacgatgct
     3001 gggccagcgc ctggtcggct cgtgtggtta gctatcgcac catgcgaggc atcggcaatg
     3061 ataatgtcgg catgggtgtt gtcgtacaag tcatggtgaa tgcggatcgg gccggtgtcg
     3121 ccttttctcg ccatccgctc gagccgctgg accggaaaca cttgctcatc agttcggtat
     3181 tcggccttgg agaaggactc gtaagcggtg ccctcgatgc ggatcacttc gaaatcgatc
     3241 gtgagtcttt cacaacgaca tccgaagtca ttgcccataa aagggaagcc ctccgtgcgg
     3301 atgacgaagg cgggatccgt tcttatccct tgcctgaatc ccagcgaacg gagccgagcc
     3361 tgtcgccgga gcaacgaaag caagtcgcac ggctctgtct tcgctgcgaa acgcggatgg
     3421 gttcccccca agacatcgaa tgggccttcg aaaacgactc gctttttctg cttcaatccc
     3481 gtcccatcac cacacttcct cccgatgcct ttttcgaccc agcggtcgtg ggccgtgagg
     3541 ctaccttatg ggataattcc aatatcgtag agagctattg cggagtcacg acccccctga
     3601 cctttagctt cgcctcctcg gcctatcgac aagtatatat ccaattctgt gaggtcatgg
     3661 gcgttcctcc ccaggaagtc gaaacgcgag aagccatgtt ccgtaatatg ctcgggcttc
     3721 ttcggggacg ggtttattac aacctcatca attggtatcg cttgttgatg ctttttccgg
     3781 gggtggcgga caacaacgcc ttcatggaaa cgatgatggg ggtgaaagac agtatgaacc
     3841 ccgagcttgc ggggatgttc gaattcatga agcatccgcc gcattattcg accttacgac
     3901 gggccaaact cgcggggatc accctggtgc gattcgcgcg tatcgactcg atcgtcaggg
     3961 aatttcaaga gtatttccgc gatgtatacg atcaagcccg tgcgactccc atccaggaca
     4021 tgggcctcgt ggcgctcgcc tcgcattatc aacacctgga agacaagctg cttcgacgat
     4081 ggtaggcccc gatcatcaac gattatttca ccatggtttc ttttgggttg ttgaagaaat
     4141 tgacggatca atggcttggg gatagtcctg aaatcaacaa cttacacaac gatctgctgt
     4201 gtggcgaggg ggatctggag agcacggagc ccaccaaatc gttgatgcgc atcgctcgcc
     4261 gggtggatga aggccctacg gagacccgtt cactattgtt ggaaaacccc gcttccgagg
     4321 tctggtctca actattgcag ggaaaagcgc cgcaacttcg gaaagacttc gaggatttcc
     4381 tcgaccggta cggattccga tgcgtcaacg aactcaagct cgaagagccg gatttgcacg
     4441 atgaccccag tttcgccgta caagcagtgg cgagctacgt ccgaatgaaa gcctattcga
     4501 tcgaggccat ggaggaaagg gaacaatcca tcagaaatca agcggaaatc gccgtacgga
     4561 gccgtctggg gggcccgcga cgactcgcct atttctgggt cgtccaacaa gcgcgtaaag
     4621 cggtccgaca ccgcgagaat ctccgcttcg cacgaaccaa agtctttgga atcgttcgac
     4681 atttattccg agccatggga gaccgcttcg ttcgcctcgg cgttcttcaa gacccccacg
     4741 atatcttcta ccttaccgtc gacgaaatct tcggattcat cgaaggaaga cccagctcgc
     4801 tggccttcct tcccctcgtg gaggccagaa agcgcgaatt tgcagcgtat cgacaaggcc
     4861 ccggcctacc ggatcgcttt ttgacctacg gagccgtcgg cgcatccctc ccctaccccc
     4921 aagtcctgca cgccaccgac ttgctacgcg gaaaaaacca aagccaggac ccgtcgctgt
     4981 tgacggggat cccttgctgc cccggcgtcg tcgaaggcac cgtccgtgtg gtgcgcgaaa
     5041 tgaaagatgc ggaaggactg catggcgaaa tcctcgtcac ggaacgaacc gaccccggct
     5101 gggtccccct ttacccctct tgctccgccc tgctcatcga acggggcagc ctcctttccc
     5161 attccgccgt cgttgcccgc gaactcggat tgcccgcggt cgtcggcatc tccggggggc
     5221 tcatgcaaaa actcaaaacc ggggatcgtg tccgggtcga cggcgcggca ggtacggtta
     5281 ccttgatttc ctgatcttca tgcctcccag ctcgcacgcg gcatcctcaa tcgctagctt
     5341 cgactcttcc caaccatggc gccatggcct ccaaagtcgg cctatccttt gcgaaacgcc
     5401 ttgttcctcc ggccctgcat cgcccgaacc ccggcacccc gaactccccc ttgattcccc
     5461 gtaggctcgg aaaaccatcc ccttgaacgg gaaacgagct acgctgcctc ccatgacggc
     5521 ccttcatatc cgatgccttt ccgaatctca gccctccgag ttcgtaacgt cgttttgcga
     5581 aatcccctat accttacgcc gcgacccatt ccgggtgggg ttgttgcccc cggaggcgga
     5641 agctcttttg ctgacactgc cccttccgaa ccttcgcgaa ttctggatcg ccactcggca
     5701 gcaacgaatc gtcggtcgca ttggtgcttc gctctccgcc gacggaaccc aaggattggt
     5761 tggttttttc gaagtcgatc tcgacgacga aaccgctgcc cggcaattgc tcgaaaccgc
     5821 cgaggcgtgg cttcgttgcc gtggtatccg caaggtcttc ggccccgtgc acataaatac
     5881 gtggttcccc tatcgattcc gggtcgaagc ccacgatgag ggggcgtact cgtgggagcc
     5941 ggtgcagccg cccgaatatc ctgaacaatt tcaagcattt ggctttaggg tggatcaagt
     6001 ctatcatagt gatgaactcg tggatctgga ggccatccga aagggattcg agcctcatta
     6061 ccgacgaacg ctgcaagccg gttataccta ccgccccatc gacacggtcg aactcgaacg
     6121 ggagttgcct gtgttgtatc gtctgagcct gagcgggttt tcgcaaaact acctatttga
     6181 accgatctcc gagcctgtgt ttcgagcact ttatgtggcg caagcggcga aaagaaaacc
     6241 gctcggatgc cacttcgtct gccaccccac gcggggagaa gtggggttta cggtgggatt
     6301 tgtcgatgaa gcggagcaat tcgtcctcaa aaccctggtg gtcttgccgg aggtgcaaag
     6361 ccaggggttg tcgatgggat tattattcga ggctttcggc tccggtgttc gcgcgggcca
     6421 tccctcagcc attgcagccc tggtccaggc gggcaatcga agcgaagcgt cgtcccgtcg
     6481 aacaccgatt ggatggcggc acacctatgc tctttttcga aaagatctgg gcgaatgatg
     6541 gagcatcagc aggctcggag acgatgagcg cctacagcga acagccgtcc aagtattcga
     6601 aatgactatg taatactctt ggaaggtatt cgtaaacatt aacgccttgc gcaatcgtca
     6661 acataccaag ccggtcgatc gcataatcga agctggcaag atcgaagccg cgaacgaatg
     6721 tggcggcatc gagcgagcgc tgagcatgct tttcgagggc tcgtcgatag gtttccacgt
     6781 attccagcca accctcgcgg gaggtaccgg ggggtaatac gaaagagagg aactcgcaga
     6841 tatcgcgttg cggcagatgc atggtggcca attcccaatc gtaagcgcac aatcggaagc
     6901 cactgcccgt ccgtcgcaag cagatattgc gcaagttgaa gtcgttgtgg atgagcgtgc
     6961 gaggagcggc ctcgatttca cgccacaagg ggccaatacg ttgaatagct cgtgataagt
     7021 aggcaagacg cggtggagtg aatagggcgg gaaattgttt tgcgttgtat tgtaggatgg
     7081 cttcccacag gggtcccatt tcttgttttg
//
LOCUS       DAOSOR010000289         7102 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10598_length_7102_cov_3.954165, whole genome shotgun sequence.
ACCESSION   DAOSOR010000289 DAOSOR010000000
VERSION     DAOSOR010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10598_length_7102_cov_3.954165"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="PK710_14990"
     CDS             <1..890
                     /locus_tag="PK710_14990"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HOT11083.1"
                     /translation="YGLYQMLMSYRLGATLVLERSFTFPAQVLKVLVEEKVTSFPGVP
                     TIFAILGELKNLSDYDFSHIRFVTNTAAALPVKHIDLLRKIFPDARVYSMYGLTECKR
                     CTYLPPEDIDKKPTSVGIAIPNTEMWIVDEQDRRVGPHEVGQLVIRGATVMKGYWEKP
                     EQTAKKLRPGPLPGELVLYTGDYCKMDEDGYLYFVGRMDDVIKSRGEKVAPKEVENAL
                     VHIAGVKEVAVIGVADELLGQAVKAFVVPEQGAVLTERQIIGEAQQRLENFMVPKYVQ
                     IVDELPKTTTGKIKKTDLR"
     gene            887..1714
                     /locus_tag="PK710_14995"
     CDS             887..1714
                     /locus_tag="PK710_14995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HOT11084.1"
                     /translation="MNDWQRNVLDALVQAGLEEPQKDADRIIEVASESENPSEAAREM
                     LERRLRGELLEYVIGRVRFMDIEMLAEPGALIPRLETEILGRGAESRIRALGVDGELR
                     IIDMCCGAGNLACALAIRFPNARVWASDLTDGCVRVARKNVQYLALQERVKVWQGDLF
                     SSLEGQDLEATIHAIVCNPPYISTGRLDKDRAHLLEHEPVEAFDGGPYGLTIHQRVIK
                     EGLPFLRSGGILGFEFGVGQERQIELLFRRAKGMDSLEFDCDADGLPRAAFTRKKGE"
     gene            1718..1963
                     /locus_tag="PK710_15000"
     CDS             1718..1963
                     /locus_tag="PK710_15000"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOT11085.1"
                     /translation="MDTADRVRQFIKDNFYAASASDLTDDASLLDLGIVDSTGILEVV
                     AFLEDEFQITVDDAEMLPENLDSIQNIVAFVERKTTA"
     gene            2035..3360
                     /locus_tag="PK710_15005"
     CDS             2035..3360
                     /locus_tag="PK710_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOT11086.1"
                     /translation="MTTEYKSELLKKIQQRDVHVGVIGLGYVGLPLALSFPEKGIRVT
                     GFDIDPRKVEMLMKGETYIMHMDANRVARALKDKLLDATSDFGRLHEPDALLIAVPTP
                     LTPQREPEMKYVVTTAGQIRDRLRKGQLVVLESTTYPGTTDELVRGILEQTGLVCGKD
                     FFLAFSPEREDPGNKQYNTKTIPKIVGGVDESSGEIAANLYGLAIDSVVQVSSARAAE
                     ATKLTENIFRAVNIALVNELKVVYDRMGIDVWEVLDAAATKPFGFMKFTPGPGLGGHC
                     IPLDPFYLTWKAREYGVSTRFVELSGEVNVAMPRYVIDKLQLALNERGLALKNAKILL
                     LGLAYKKDIDDPRESPSFELIDILLRRGALVTYHDPHISLAPKMRSWPDLPAMHSVPL
                     DAQTLATADAVLIATEHSSVDYDLVLKHAKLVVDTRGVFRKPKPNVVKA"
     gene            3387..4322
                     /locus_tag="PK710_15010"
     CDS             3387..4322
                     /locus_tag="PK710_15010"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT11087.1"
                     /translation="MMDFTGRGVLVTGGAGFIGSHLVDELVTRGARVRVLDNFFSGRR
                     ENLASSIDRIDLLEGDIRDISTCQAAMNNIDVVFHQAALGSVPRSMNDPATSIDVNIG
                     GTANIFSAARDAKVRRLVYASSSSVYGDSTRLPKKEGEEGKPLSPYALSKQVDEQIAD
                     TFARCFGMEVVGLRYFNVYGPRQDPSGPYAAVVPRFFAACIQGQSPTIFGDGMQSRDF
                     TFVADVVRANLLAASAPPASCGRAYNVGAGGTTRINDLARTIIEVTGANVQPRHEPAR
                     AGDVPFSQADVTDAKTMLGFEATTRLKEGLLATKP"
     gene            complement(4326..5186)
                     /locus_tag="PK710_15015"
     CDS             complement(4326..5186)
                     /locus_tag="PK710_15015"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOT11088.1"
                     /translation="MKPIPAGTFMMGSPTSEPGRDEYNFGSSGELNETLHSVTLTRPF
                     EIGDVEVTQHDFNIVMGYNPSFFVQCGSSCPVETITFDEALAFCNVLSLAKGLPPCFE
                     CTGKKKSTSCKLSSEYGSPYECQGYRLPTEAEWEYAARAGTQTALYSGALTQLDCANV
                     DPKLGSIGWYVANSNAAYDSQTLTTCHENTYSVGTQQVRTRSANNFGLYDMAGNVWEF
                     VHDCGQPYTSASQTDPIGPLKCPSESRIFRGGGLYSVAHFCRHAERADTPGNDIDAPD
                     LGFRIVRTLP"
     gene            complement(5183..5725)
                     /locus_tag="PK710_15020"
     CDS             complement(5183..5725)
                     /locus_tag="PK710_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11089.1"
                     /translation="MTRNKAAILVLGPILCLGCTYDFDAPFAAAMPGDASADRAPDGH
                     VADSGSDVNSSDSWPVDVHGDLGGDVDGCISSCVGKCAGADDGCGHACPTDQCDEGQQ
                     CAQGVCTSCPHEVCNGVCCNAGEVCTPKERAVLPRIAIRSALNAETTTMDVAGVWLVA
                     RALRIISAWMGSHVPRSTLP"
     gene            complement(5825..6562)
                     /locus_tag="PK710_15025"
     CDS             complement(5825..6562)
                     /locus_tag="PK710_15025"
                     /inference="COORDINATES: protein motif:HMM:NF039851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5765 domain-containing protein"
                     /protein_id="HOT11090.1"
                     /translation="MCWSGEASAAIALGGLTSAYILKRKGEPKEIYIPTAYFVLMEGL
                     QALTYIVVGECGTRFNTLFTHLAMVHISFQPIFINMLGMEFVDPGVKRRIKKYVYAFC
                     GLVAVFCLMRLIPAYELLGRCETGGPLCSHVQTCAYRGQWHIGWDVLLNGFNERWRWY
                     VVSAFALPVLYGSWKWSLYHYVVGPLAASLTTSDVNERPAVWCLFSTCIIALLVNTRL
                     RNYIHVRKWFTWKYLLARTPTRMASVD"
     gene            complement(6718..>7102)
                     /locus_tag="PK710_15030"
     CDS             complement(6718..>7102)
                     /locus_tag="PK710_15030"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HOT11091.1"
                     /translation="RLYTASTEKMRYDTIPTAVFTPVEIGVVGLTEAQAIDRFGSESV
                     KVYRAEFKPMRKALMPDSPVRAFVKMVVAHREEKVVGLHLIGPEAAEIIQGFSVAITA
                     GLTKKQFDDTLAIHPSLAEEAVTLR"
BASE COUNT         1621 a         1915 c         2007 g         1559 t
ORIGIN      
        1 actacggcct gtaccagatg ttgatgtcgt atcgtcttgg ggcaacgtta gtcctcgaac
       61 gttcgtttac gtttccggcg caggtgctca aggtgctggt agaggaaaaa gtcacgtctt
      121 ttccaggagt tcctacgatt ttcgcgattt tgggagaact caaaaaccta tccgattacg
      181 acttcagtca cattcgattc gtgacgaata cggcggcggc gcttccggtc aagcatatcg
      241 atttgcttcg caagatcttt cccgacgcgc gggtttactc gatgtacggg cttaccgagt
      301 gcaagcgatg cacctatctg cctcccgaag acatcgacaa aaagcctacc agcgtgggga
      361 ttgccattcc caatacggag atgtggattg tcgacgagca agaccggcgg gttgggcccc
      421 atgaagtggg gcaactggtc attcgtggtg ccacggtgat gaagggctat tgggaaaagc
      481 ccgaacagac cgcgaaaaaa ttgaggcccg gtccgttgcc cggcgaactc gtgttgtata
      541 cgggcgatta ctgcaagatg gatgaggacg gatatttgta cttcgtgggc cgcatggacg
      601 acgtcatcaa gagtcgtgga gagaaagtcg cgccgaaaga ggtggaaaat gcacttgtcc
      661 acattgccgg tgtgaaagag gtggcggtca tcggcgtagc ggatgaatta ttgggtcaag
      721 cggtcaaggc gttcgtggtt cccgaacagg gggcggtgtt gacagaacga cagattatcg
      781 gcgaagcaca gcagcgttta gaaaacttca tggtacccaa gtacgtgcag atcgtggacg
      841 agcttccgaa aacgaccacg ggaaaaatca aaaaaacgga tttgcgatga acgattggca
      901 acgaaacgtg ctcgacgcgc tggtgcaagc agggctcgaa gagcctcaaa aagatgcgga
      961 tcgcatcatc gaggtagcgt ccgagagtga aaacccttcc gaggctgcgc gggaaatgct
     1021 ggagcggcga ttgcggggcg agctattgga atacgtgatt ggtcgcgttc ggttcatgga
     1081 catcgagatg ttggcggagc cgggagcgtt gattcctcgc ctcgagacgg agatcctcgg
     1141 acgaggtgcc gagagccgta ttcgtgctct tggggtcgac ggtgagcttc gcatcatcga
     1201 catgtgttgt ggggcgggca atctcgcctg tgctcttgcc attcgattcc cgaatgcgcg
     1261 ggtatgggcc tcggacttga cggatggttg tgttcgtgtg gcaagaaaaa atgtgcagta
     1321 cctggcgtta caagaacgcg tgaaagtatg gcaaggagac ttgttttcat ccttggaagg
     1381 acaggatctc gaagccacga ttcacgccat cgtatgcaat ccaccctata tttccaccgg
     1441 tcgtctcgac aaggatcgcg cgcatttgtt ggaacatgag ccggtcgagg cctttgatgg
     1501 aggcccctac ggattgacga ttcatcagcg tgtcatcaaa gaaggtctgc cgttcctccg
     1561 ttcgggcggg atattgggtt tcgaattcgg agtcggacag gaacggcaga ttgaattgct
     1621 tttccgccgt gccaaaggaa tggacagcct cgaattcgat tgtgatgccg atggccttcc
     1681 gcgcgcggct ttcacccgta agaagggcga gtaaggaatg gataccgcag acagggtgcg
     1741 acagttcatc aaagacaact tctatgcggc tagcgcaagc gatctcaccg acgacgcttc
     1801 gctgctcgac ctgggaatcg tggactccac gggtatcctc gaggtcgtgg cgtttctcga
     1861 ggatgagttc cagatcacgg tcgacgacgc cgagatgctt ccggaaaacc ttgattccat
     1921 tcagaatatc gttgctttcg tggagcgaaa aacgacagcc tgagcgcgcc tgatcacatt
     1981 ttgaatcatt ggggataggg aggcagttct gcccccggaa atcgaggaag catcgtgacg
     2041 acggaataca agagcgaatt gctcaagaag atacagcaac gcgacgttca cgttggggtc
     2101 atcggactcg gctatgtcgg tcttcctttg gccctgtctt ttccggaaaa gggaattcgc
     2161 gtcacgggat tcgatatcga cccgcgaaaa gtcgagatgc tgatgaaggg tgagacctac
     2221 atcatgcaca tggatgccaa ccgtgtggcg cgcgccttga aagacaagct gctcgatgcc
     2281 acttccgatt ttggtcgttt gcacgaaccc gatgccttgc tcattgccgt tcccacgcct
     2341 ttgacaccgc agcgggaacc ggaaatgaaa tatgtggtaa cgaccgcggg tcaaatccga
     2401 gaccgcttgc gaaaaggaca gctcgtcgtt ctcgaatcga caacctatcc gggaaccacc
     2461 gatgagctgg tgcgcgggat tttggaacag acagggctgg tatgcggcaa ggactttttc
     2521 ctcgctttct caccggagcg agaggacccc ggcaacaagc aatacaatac gaaaacgatt
     2581 cccaagatcg tgggtggcgt ggatgagtca tcgggtgaga ttgcggccaa tctttatggc
     2641 ctcgcgatcg actctgtcgt tcaggtttct tcggcgcgcg cggcggaagc caccaaactg
     2701 acggaaaaca tctttcgtgc cgtcaatatc gctctcgtca atgaactcaa ggtcgtgtac
     2761 gaccgcatgg ggatcgacgt ttgggaggtg ctcgatgcgg cggctaccaa accctttggg
     2821 ttcatgaagt tcacgccagg tccggggctc ggaggccatt gcattcccct tgatccgttc
     2881 tacttgacct ggaaggctcg cgagtacggc gtttccacac gtttcgtcga attgtcgggt
     2941 gaggtcaatg tcgccatgcc gcggtatgtc atcgacaagc ttcaactcgc cctcaacgaa
     3001 cgtggccttg ctctcaaaaa cgccaagatc ctgctacttg ggctcgcgta caaaaaagac
     3061 atcgatgacc ctcgggaaag cccttcgttc gaactcatcg acattcttct tcgaagaggt
     3121 gccctggtta cctaccatga cccccacatc tctttggcgc ccaaaatgcg ctcctggcca
     3181 gacctacccg ccatgcattc ggtgcctctg gatgcacaaa cgctggccac tgccgacgcc
     3241 gtgctcatcg ctaccgaaca tagctccgtc gattacgacc tcgtgttgaa acacgccaag
     3301 ctcgtggtcg atacccgcgg cgtatttcgc aaaccaaaac caaacgtcgt caaagcctga
     3361 gcccttggga gcaaaaagac atggacatga tggacttcac gggacggggg gtattggtta
     3421 caggaggcgc gggattcatc ggttcccatt tggtggatga actggtgaca cggggcgctc
     3481 gcgtgcgcgt tctcgacaat tttttcagtg ggcgacgaga aaacctggct tcgagcatcg
     3541 atcgaatcga tcttctggaa ggcgatatcc gcgatatttc tacctgccag gcggcgatga
     3601 acaacatcga tgtcgtattc catcaggcag ccttgggctc tgttcctcga tccatgaacg
     3661 acccggccac gagcatcgat gtcaatattg gagggactgc gaatattttt tccgcggccc
     3721 gggacgccaa ggttcgtcgc ctggtgtacg cctcttcttc gtcggtctac ggcgatagca
     3781 cccggctccc caaaaaagag ggagaggaag gcaaaccctt atcgccctat gcgttgtcaa
     3841 aacaggtcga tgagcaaatc gcggatacct ttgcccgttg tttcggcatg gaggtggtgg
     3901 ggctgcgcta cttcaacgtt tacggtccac gacaagatcc ttctggcccg tatgcggcgg
     3961 tcgttccaag gttttttgcc gcgtgtattc aggggcagtc accgaccatt ttcggcgatg
     4021 gcatgcagag ccgggacttc acgttcgtgg cggatgtggt gcgtgcgaac ttgctcgcgg
     4081 catcagcgcc accggcttcc tgtggtcgtg cgtacaacgt gggcgcggga ggtacgaccc
     4141 gcatcaacga tctggcgcgt acgattatcg aggtcacagg agccaatgtg caaccgcgcc
     4201 atgaacccgc acgcgccgga gatgtgccgt tttctcaagc ggatgtgacg gatgccaaaa
     4261 ccatgctcgg attcgaagcg acgacacgtc tgaaagaagg cttgctggcg accaagccat
     4321 aaacctcagg gcaaggtccg gactatacga aatcccagat cgggcgcatc aatatcattg
     4381 ccgggtgtat ccgctcgttc cgcatgacga caaaaatgcg ccacgctata aagtccgcct
     4441 cctcgaaaaa ttcgagattc acttgggcac tttagcgggc caattggatc ggtttggctg
     4501 gcggacgtgt agggctgccc gcaatcatgc acaaactccc atacatttcc ggccatatcg
     4561 tataaaccga agttgttggc cgaacgtgtg cgaacctgtt gggtccccac ggagtaggta
     4621 ttttcatgac acgtcgtcaa ggtttggctg tcgtaagccg cgttcgaatt cgcaacgtac
     4681 catccgattg agccgagctt cggatcgaca ttggcgcaat ccaattgcgt cagcgcaccc
     4741 gaatacaatg ccgtttgtgt tcccgctcgg gcggcatatt cccattcagc ctcggtgggc
     4801 agtcgataac cctggcactc gtaggggcta ccatactcgc tagacagctt acaagacgtg
     4861 gactttttct tacccgtaca ctcgaaacac ggcggaagac cctttgccaa cgacaagaca
     4921 ttgcaaaacg caagggcttc gtcgaacgtt atcgtttcca cagggcaact ggagccgcac
     4981 tgcacaaaga acgatgggtt gtaacccatg acgatattga agtcgtgttg ggtcacttcg
     5041 acatccccga tttcgaacgg gcgcgtgagg gttacggaat gaagggtttc attgagttca
     5101 cccgacgaac cgaagttgta ttcatctcgt ccgggttcgc tggtggggct tcccatcatg
     5161 aacgtgccgg cgggaattgg cttcatggca aggtcgagcg cggaacatga cttcccatcc
     5221 aggcagaaat gattcgcagc gcacgagcca caagccatac tcccgccaca tccattgtcg
     5281 tggtttccgc attcaaggct gagcgaatcg caatcctggg gagtacagca cgttccttcg
     5341 gggtacaaac ctcaccggcg ttgcaacaga caccgttgca tacttcgtgt gggcacgatg
     5401 tgcatactcc ctgagcgcat tgctgcccct cgtcacattg atcggtcggg caagcatgcc
     5461 cacaaccatc gtccgcaccc gcgcacttac ccacgcacga tgatatgcat ccgtcaacgt
     5521 cgccaccgag atcgccatga acatccacgg gccaactgtc cgaagagttc acgtcggacc
     5581 ccgaatcggc aacgtgaccg tctggcgccc tgtccgcgct agcatcgcct ggcatggcgg
     5641 ctgcgaacgg agcatcgaaa tcatacgtgc agccaaggca aagaatgggc cccaagacca
     5701 aaatggcagc cttattccga gtcatgcctg accgtttcca catcatcggt gtattgtgcg
     5761 tcgggcgaaa ttccttggca agacgagctt tggatcctgc cgtccgcagg caagatgggg
     5821 agggtcagtc cacgcttgcc atgcgtgttg gcgttcgagc aagcagatac ttccatgtga
     5881 accactttcg aacatggata tagtttctga gccgcgtgtt gaccagcaaa gcaatgatgc
     5941 acgtactgaa caaacaccaa accgccgggc gttcgttgac atcgctggtg gtcaacgatg
     6001 ccgccaaagg accgaccacg taatgataga gcgaccactt ccacgagcca tacagcacgg
     6061 gtaaggcaaa ggcagaaacc acgtaccatc gccatcgttc gttgaatccg ttgagcaaca
     6121 catcccagcc gatatgccat tgccctcgat aagcgcaggt ttgcacgtgg ctacaaagcg
     6181 ggccgcccgt ctcacagcgc cctaacaatt cgtaggcggg aatgagccgc atcaagcaga
     6241 acacggccac gagcccacag aaagcatata catacttttt gatccgtcgt ttcaccccag
     6301 ggtcgacgaa ctccatgccc aacatattga tgaagatcgg ttgaaaagaa atgtggacca
     6361 tggccagatg ggtgaacaac gtattgaagc gtgttccgca ttctcccacc acgatatacg
     6421 tcaacgcctg aaggccttcc atcaacacga aatacgcggt gggaatgtag atctctttcg
     6481 gttcgccttt tcgtttgagg atgtaggcgc tcgtcaatcc tcccaacgca atggcggcgc
     6541 ttgcttcacc actccagcac atgcatccct atcctttcga cagccatggt tggatgtcca
     6601 ttggtttcat gggtgcgccg gacactaaca cgcgggccat gcgaacggga cggattgctc
     6661 acatgatttc gtgaggctgc attcattcgt tcggacggta caatgaatcg aatgggtcta
     6721 tcgcaaggtg acggcttctt ccgcgaggct tgggtgaatc gcaagggtgt cgtcgaattg
     6781 ttttttggtc agccccgcgg tgatcgcaac cgagaaaccc tgaataattt cagcagcttc
     6841 cggcccaatc agatgcaagc cgaccacctt ttcttcgcgg tgggcgacca ccatttttac
     6901 gaaagctcgg accggcgaat cgggcataag agctttgcgc atcggtttga attcggcgcg
     6961 atagactttg acggattcgc ttccgaatcg gtcgatggct tgcgcttccg tgagtcccac
     7021 gacaccgatt tcgacgggcg tgaagacagc ggtggggatc gtatcgtacc gcattttctc
     7081 ggtacttgcg gtgtacagac gg
//
LOCUS       DAOSOR010000290         7088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10643_length_7088_cov_4.142613, whole genome shotgun sequence.
ACCESSION   DAOSOR010000290 DAOSOR010000000
VERSION     DAOSOR010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10643_length_7088_cov_4.142613"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PK710_15035"
     CDS             <1..194
                     /locus_tag="PK710_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11092.1"
                     /translation="PIPSQSGMYDHRQRRFDLSLANPISCISSMTAARHEIMTTGPDA
                     IVWDRCARVTTSLIDVCAA"
     gene            230..1237
                     /locus_tag="PK710_15040"
     CDS             230..1237
                     /locus_tag="PK710_15040"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOT11093.1"
                     /translation="MLDAKLALETLLELTRALRGERPLEEALGEVTRAAVRLLSANHA
                     SLRLLDDSGEHLLAAVRSGAGSDRPALSFRRGEGVLGWVVDHGEAVLLQDARHDARFK
                     RNPAKQGFVVGSLMVVPMLAAGRVFGALSCSSDRTDAFSEEELQLLQLLANCSVPPIE
                     KARLERLAVIDPHTLAYRQTYLLPRLEEELGRASRYATPLSLLFLDLDHFKNINDSYG
                     HAAGDRVLRTFADRVRQSIRLSDLLIRRGGEEFVVVLPNMPLDGAKAAAERIRAHIEN
                     TRFVIGDDQSITQTVSIGVAQWDGVELAAHLEERADQAMYAAKTLGRNRVCLSTPPTN
                     P"
     gene            1267..2598
                     /locus_tag="PK710_15045"
     CDS             1267..2598
                     /locus_tag="PK710_15045"
                     /inference="COORDINATES: protein motif:HMM:NF025065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOT11094.1"
                     /translation="MASSLPMNLIEWIDDDVRASYASRLGLLAQHGIVEHHASIPFAG
                     ILSNTLSQRRACVLFDTRTYDAAGATCCRALEDSGFDVTELLLPDPNEKGPVCDDHTL
                     RWIVDRLPPVDIVVAVGSGVINDLAKWTACANNVAYAVFATASSMNGYSSSNVAATVQ
                     GVKTLVPGRAALAIGADPYILASAPHRLTTAGFGDLLAKAVSIADWILNAMVAKETFC
                     PRLTSWAERWAEPVLVRPEAIASEEPQAIQALFHALVVSGCVMTLHGSSLPASGAEHV
                     ISHTLDMMSDADGQPHDLHGRQVGVATIVAAALWEHMLHLRRPTFKPFMPPWDANVWG
                     KSAPAVAEHLSKKTEVIHKTCEKLSASGYWEHIQENVRPHLRSPQWVKDQLARANAAH
                     QLSHIGCSRERFLMALRHASAMRSRLTCLDIVRMAGVVPEVIENIVDRWVA"
     gene            complement(2635..3495)
                     /locus_tag="PK710_15050"
     CDS             complement(2635..3495)
                     /locus_tag="PK710_15050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT11095.1"
                     /translation="MNDDETMRSEAASITRRFSFARGCYESLFVLFPPCASLSCHGQT
                     QGVCMAQGDHEVLNVLVIDDDEAVRSLFVDILSREEHQAVAAASAEQGLELLPFWTFQ
                     VAFIDQHLPGMDGIVLGEYLRKNNPDMMIVIITGDCDERVERRSKDLSLRFLAKRFRV
                     QDILDVLDAWFEASRERERISSIRGGVDFEPALASFVQDLPACFAMPNVPARIEDRIV
                     TTIRRCLNDLRSSRRYTERDRVVALAGLLAAQVLGIQLPRTSQGMTLFQEYDEIMKER
                     GKATAFETEG"
     gene            3629..5545
                     /locus_tag="PK710_15055"
     CDS             3629..5545
                     /locus_tag="PK710_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11096.1"
                     /translation="MSKTSCRALMRHLSLLIIVIASIAGCSSDGDDSQGGPNTNGSSG
                     TDLIVRGHITGVSFGMGAPPPPLGNVDVAAGIDRNGDGKITDDEMVHASSDFNGDYTV
                     SVGVSEGDHVVMSYRYEGLAPHYRRIIASGPGQTVIDVNMAELKPLKCEANACLLEGN
                     QLRIDGLPQGTSGFAKLFNPVAQPEAFPGAFEESTGKLLISGVFSVVELEDANGNALR
                     DLPSPATLRMTVPVDTWNIIIDIHPGTDRIEVPLYAFDENIGTWVRDGEAYLEDSQGV
                     IIPESSLPAIRDGSYDDTVVSVGDVSHFSYWNVDWPVESHGCVSGVVLDENGKIAVGA
                     TVFVRGATYTGSSSPQTIGNDGRFCVDVMRSEGPGEDLDQDGVTGETHSVSIRVSYQN
                     KVFDLGKVDIDATAATCSEGGCTDMGDIRLTNDKTLQLALCTVSGFIKDVKGNPVSGA
                     SVFAYDDSVESEWLTSVCGQAFENCTLLATSEADGSFVITTGILETMTLMAMYSTDPE
                     EGHHVFRFSERWFPDCPSGPITVTLDQGYDSIELSLSISGNEISWTPNLPVNLLMVSD
                     SEGDNKWMLTGKEPPGFTSPITYGSVPPKATQVAPAQGQPAALASEDTISIAGSATTS
                     DGYPLAISGSATIP"
     gene            complement(5613..6212)
                     /locus_tag="PK710_15060"
     CDS             complement(5613..6212)
                     /locus_tag="PK710_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11097.1"
                     /translation="MTHSRHKSGTSTTPMSSEEELQSVDPTERFVRTDESLDAIVLPS
                     VDSEGELAVTQPFLKASLGEEDEDLIRTRELPIRGEDWFGASKGGQENPKTMPTGENM
                     GAFSHPALGARLAVSRSAPRSATSKPQAADAQIRAAYAKYVELCHATGKPAVVEDRFT
                     ARLAERLEQLSISYPREQIEFSPTLDGETVRVRVLLRRR"
     gene            complement(6337..6786)
                     /locus_tag="PK710_15065"
     CDS             complement(6337..6786)
                     /locus_tag="PK710_15065"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOT11098.1"
                     /translation="MTKATFEAELLPHLADILMGGAHADEHLDGREVDAVVELLTGLI
                     KDADELPEPLRNRIDAFDPEQFDIKRTAYAMGDLSKEQRRAVLEMLSDLSEVDDEIDL
                     KEDEYLRAVADALGSSTEELEGLVVEIELISTPSRPVPPPLPPRSKP"
     gene            complement(6904..>7088)
                     /locus_tag="PK710_15070"
     CDS             complement(6904..>7088)
                     /locus_tag="PK710_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11099.1"
                     /translation="VTGPGIKPEGIQRVITDWKPWVILSARDGAEYKVDMQLLDPSGE
                     AVPYGAVSRTFTVKRP"
BASE COUNT         1576 a         2175 c         1825 g         1512 t
ORIGIN      
        1 accccatccc atcgcaaagc ggaatgtacg accaccgaca acggcgtttc gacctttccc
       61 ttgccaaccc aatctcatgt atctcctcca tgactgcggc tcgacacgaa atcatgacga
      121 ccggtcccga cgcaattgta tgggatcggt gtgctcgggt cacgacaagc ttgatcgatg
      181 tgtgtgcggc ttgacttcat caccacgggt cgccctagta tggactttga tgctggatgc
      241 gaagctcgca ttggagacat tgctggaact cacccgcgcg ttacgtggtg agcgaccgct
      301 cgaggaagct cttggtgaag taacgcgagc cgccgtccgc ttgctcagcg ccaaccacgc
      361 ctcgttacgc ctgctcgacg atagcggaga acatttgctt gccgccgtac ggtctggagc
      421 tggctccgat cgtcctgccc tgagctttcg gcgcggtgaa ggcgtactcg gctgggtggt
      481 tgaccatggt gaggctgtcc ttcttcaaga tgcgcgtcac gatgcccgtt tcaaacgcaa
      541 tcccgccaaa caaggatttg tcgttggctc cttgatggtc gtgcccatgc tcgcggccgg
      601 acgcgtcttc ggagctttgt cttgttcttc cgatcgtaca gacgcgtttt cggaagagga
      661 attgcaacta ttacagttgc ttgccaattg ctctgttccg cccatcgaga aagcaagact
      721 cgaacgcctt gccgtcatcg acccccacac ccttgcttat cgacaaacct acttgctgcc
      781 ccgcctcgag gaagaactcg gtcgagccag tcgctatgcc acacccttga gtttgctgtt
      841 cctggacctc gaccacttca agaacatcaa tgattcgtac ggccacgctg ccggcgaccg
      901 tgttcttcgc acattcgccg atcgcgtacg ccagagcatt cgactgtctg acttgctcat
      961 tcgacgcggt ggtgaggaat tcgtagtcgt cctgcccaac atgccattgg atggcgccaa
     1021 agccgccgcc gaacgcatca gagcacatat cgaaaacacc cgtttcgtca tcggcgatga
     1081 ccaatccata acgcaaaccg tatctatcgg cgttgcccaa tgggatggtg tcgaactcgc
     1141 cgcgcacctg gaggaacgcg ccgaccaggc catgtacgcc gccaaaaccc taggacgaaa
     1201 ccgggtttgc ctgtccacgc ctccgacgaa tccataacgc ctcacctttc cgactcaacc
     1261 cctcgcatgg caagctcact ccccatgaat ctcatcgaat ggatcgatga cgacgttcgt
     1321 gcctcgtatg caagccgtct aggcctgctt gcccaacacg gaatcgtcga acaccacgcc
     1381 agcatccctt tcgccgggat cctgtcaaac accctttcgc aacgacgcgc ctgtgtcttg
     1441 ttcgacacca gaacctacga cgcagcagga gcaacctgct gtcgggcgct ggaggactcg
     1501 ggcttcgacg tcacggaatt gctccttccc gatcccaacg aaaaagggcc cgtttgcgac
     1561 gaccacacgc tgcgttggat tgtcgatcga ttgccccctg tagacatcgt tgtcgcagtg
     1621 ggaagcggtg tgatcaacga ccttgccaag tggaccgcct gcgcaaacaa cgtggcgtat
     1681 gccgtcttcg ctaccgcatc atccatgaac ggatactctt cgtccaatgt ggcggcgacg
     1741 gtgcaagggg tcaaaacact ggtgccgggg cgtgcagcac tggcgattgg ggccgatcca
     1801 tatattctcg catccgcgcc tcatcgcctg accacggctg gatttggtga cctactcgca
     1861 aaagccgtca gcattgccga ctggatcctc aatgccatgg ttgcgaaaga aacgttctgt
     1921 ccccggttga cgtcgtgggc agaacggtgg gccgagcccg tcttggtacg gcctgaagcc
     1981 atcgcctccg aagaaccaca agcaatccaa gccctattcc atgccctcgt tgtctccgga
     2041 tgcgtcatga cacttcatgg ctcgagtctg cccgcatccg gagcggaaca cgtcatcagt
     2101 cacaccttgg acatgatgag cgatgccgat ggacaaccac acgatctgca cggacgacaa
     2161 gtcggcgtcg ccaccatcgt ggcagccgct ctgtgggagc atatgcttca tcttcgcaga
     2221 cctacgttca aacctttcat gcctccttgg gacgccaacg tatgggggaa atccgccccc
     2281 gccgtagccg aacatctttc gaaaaagacc gaagtcatcc acaaaacttg tgagaaacta
     2341 tccgcatcgg gatattggga gcatatccag gaaaacgttc gtccgcattt gcgatcgccc
     2401 caatgggtca aagaccagct cgcgagagca aacgcggctc accaactcag ccatatcggt
     2461 tgttcgcgag aacgcttctt gatggccctc cgacacgcaa gcgccatgcg atcacgcttg
     2521 acatgcctgg atatcgttcg aatggcaggc gtggttccag aagtcatcga aaatatcgtc
     2581 gaccgttggg tagcataagc cctttacccc acggcctccc acccaacgaa cctctcatcc
     2641 ctccgtctca aaagccgttg ctttgccacg ctctttcatg atctcgtcat actcctgaaa
     2701 caaggtcatg ccttgactcg ttcgcggaag ctgaattcca agaacttgcg ccgcaagcaa
     2761 acccgccaac gccaccacac gatcgcgctc cgtgtatctt cgcgaactac gcaagtcatt
     2821 gagacaacgt cggatcgtgg tgacaattct gtcttcgatt cgcgcgggaa cattcggcat
     2881 cgcaaaacaa gccggaaggt cttgcacgaa actagccaac gccggctcga aatcaacccc
     2941 tccccgaatc gacgaaatcc tctcgcgctc tcgactcgcc tcgaaccatg catcgagcac
     3001 gtcgagaata tcctgaacac gaaatcgctt cgccaaaaac cgaagcgata gatccttgct
     3061 gcgacgttcc acgcgttcgt cacagtctcc ggtgatgatg acgatcatca tgtcgggatt
     3121 gtttttccgc aaatactccc caagcacgat tccatccatg ccaggaagat gctgatcgat
     3181 gaacgcaacc tggaacgtcc aaaacggcag caattcgagc ccttgttccg cggacgctgc
     3241 ggcaacggcc tggtgctcct ctcgcgaaag aatgtccacg aaaagggaac gcacggcctc
     3301 gtcgtcgtca atgacgagaa cgttgagaac ctcgtgatcg ccttgcgcca tgcatacccc
     3361 ttgcgtctgt ccgtgacacg ataggctagc gcaaggagga aaaagcacaa acaacgattc
     3421 gtagcaccct cgtgcgaacg aaaaccgacg cgtgatcgat gccgcctccg aacgcatcgt
     3481 ttcgtcatcg ttcaaatcgt ttcaccaatc cgaacgacat cgtctgccca ccggaacatg
     3541 cttttgcagc cggagacgta ccatcaatcg aggtttcggg agggaagttt cccgtacttt
     3601 tcgcgtagat ccccagaagg agaccaccat gagcaaaaca tcttgtcgcg ctcttatgag
     3661 acacttgtcg ctactcatca ttgtcatcgc ttcgatcgcg ggctgctcct ccgacggtga
     3721 tgattcccaa ggaggcccca acaccaacgg ttcttcggga accgacttga tcgttcgcgg
     3781 ccatatcacc ggcgtgagtt ttggcatggg cgcgcctccg ccccccttgg ggaacgtgga
     3841 tgtggccgcc ggcatcgatc gaaacggcga cggtaagatc accgatgacg aaatggtgca
     3901 tgcatccagc gacttcaacg gcgattacac ggtcagtgtt ggtgtatcgg aaggcgacca
     3961 cgtggtcatg agctaccgat atgaagggct agcgccccat taccgaagga tcattgccag
     4021 cggtcccgga caaaccgtca tcgatgtcaa tatggcggaa ctcaaacctt tgaagtgcga
     4081 ggcaaacgca tgcttgctcg agggcaacca gctccgtatc gatggtttgc cacaaggcac
     4141 ctcgggattc gcaaagctct tcaaccccgt ggctcaaccg gaagcatttc ctggagcctt
     4201 cgaagaaagc acgggcaagc ttctcatctc gggcgtgttc tctgtcgtgg aactcgagga
     4261 cgccaacggc aacgctttgc gagatctgcc atcacccgcc acgctgcgta tgaccgtgcc
     4321 cgtcgatacc tggaacatca tcatcgatat ccatcccggc accgaccgta tcgaagtgcc
     4381 cctctacgct ttcgacgaaa acattggaac gtgggtgcgc gatggagagg cttacttgga
     4441 ggacagccaa ggagtcatta tccccgaatc atctctgccg gccatccggg acggctcgta
     4501 tgacgacacc gtggtatccg tgggcgatgt ttcccacttc tcctattgga acgtcgattg
     4561 gcccgtcgaa tcccacggtt gcgtctctgg cgtcgtcttg gacgaaaacg gaaaaatcgc
     4621 ggtgggagcc acggtcttcg ttcggggagc gacgtacacc ggcagttcat cgccgcaaac
     4681 gataggcaac gacggtcgat tctgtgtcga tgtcatgcga agcgaaggtc ccggcgaaga
     4741 cctcgatcaa gatggcgtca ccggcgaaac ccatagcgta agtatccgag tttcctatca
     4801 aaacaaagta ttcgacctcg gaaaggtcga catcgacgct accgccgcca cctgctccga
     4861 aggcggctgt accgacatgg gcgatatccg tctcaccaat gataaaaccc tacagctagc
     4921 gctctgcacg gtgtccggct tcatcaagga tgtcaaaggc aatcccgtgt ccggtgcgtc
     4981 tgtctttgcg tacgatgact cggtcgaatc cgaatggttg acatccgtct gcggacaagc
     5041 cttcgaaaat tgcaccttgt tggccacctc cgaggccgat ggctcttttg tcattacgac
     5101 aggaatactc gaaacgatga cactcatggc catgtactcc accgacccgg aagaaggtca
     5161 tcacgtgttc cgtttcagcg aacgatggtt cccagactgc ccctccggcc ccatcaccgt
     5221 cacgctcgat cagggctacg atagtatcga gttatccttg tccatttccg gaaacgaaat
     5281 ctcctggact ccaaaccttc ctgtcaatct gctgatggtt agcgactcag aaggcgacaa
     5341 caaatggatg ctcacgggaa aggaaccgcc cggattcacc agccccatca cctacggttc
     5401 ggtaccaccc aaggctactc aggtggcacc cgcacaaggc caacccgctg ctttggcatc
     5461 cgaggatacg atttcgatcg ccggtagcgc caccacctcg gatggctatc cgctcgccat
     5521 ctccggctca gctacgattc cctgacaagc ctaccgattg ccgccgatcg caaacgtgcc
     5581 gtcggcggtt gcattcctcg acgatcctgg agtcatctcc gccgcaaaag cactcgaact
     5641 cgaaccgttt ccccatccaa cgtcggcgaa aactcaattt gttcgcgcgg ataagagatg
     5701 gatagttgtt cgaggcgttc cgccaaacga gctgtaaaac ggtcttcgac cacagccggt
     5761 ttgccagtgg catgacataa ttcgacgtac ttggcgtatg ccgcacgaat ctgtgcatcc
     5821 gcggcctgtg gttttgacgt ggcagaacga ggggcagacc gtgagacggc aaggcgtgcg
     5881 cctagcgccg gatgggaaaa agctcccatg ttctcgccgg tcggcatggt cttcggattc
     5941 tcctgcccac ccttgcttgc tccaaaccaa tcctctccgc gaatcggaag ctcacgggtt
     6001 cgaatcaagt cttcgtcctc ttcacccaac gaagccttca aaaaaggttg tgtgacggcc
     6061 aattcgccct cggagtccac ggaaggcaaa acgattgcat ccaggctttc gtcggttcgg
     6121 acaaaccgtt ccgtcgggtc cacgctttgc aactcctctt ccgaagacat cggagtcgtg
     6181 gaggtcccgg atttgtgacg agagtgcgtc atgaccaaac ttgtcggcgt tggcttcagg
     6241 gcaaagcgtg ccacgtgcca ggacatccat caacgatatc gcgtcgatgg gatcctccca
     6301 gcgatggtga atcgaaaaaa atcgatgtcc gatggattag ggtttggacc gcggaggaag
     6361 aggcggcggc acggggcgcg atggcgtact gatcaactcg atttcgacaa ccaagccttc
     6421 gagttcttcc gtggatgaac ccaatgcatc ggccacagcg cgaagatatt catcctcctt
     6481 gagatcgatt tcatcatcga cttctgacag atcagagagc atttcgagca ccgcgcgacg
     6541 ctgttctttg ctcagatctc ccatggcata cgcggtgcgc ttgatgtcga attgttcagg
     6601 atcgaaagcg tcgatacgat ttcgaagagg ttctggaagc tcatccgcat ccttgatcaa
     6661 accggtcagt agctcgacca ccgcatcgac ttctcgacca tcgagatgct catcggcatg
     6721 agcgcccccc atgagaatat cagccaagtg cggcaatagc tccgcttcga acgttgcttt
     6781 cgtcatgcgg gtttcctcac tttcttgctg tgaaccgaga tgggcaccgg ctcgagacgc
     6841 tcttccggtt cgtgattgga ttgccgcaga gtatgcggtt tcgtttcacc actcaagcca
     6901 tcgtcacgga cgtttgacag tgaaagtacg gcttacagcg ccataaggaa ccgcctcacc
     6961 gctgggatcg agaagttgca tgtccacctt gtattccgcg ccatctcgag ccgacaaaat
     7021 aacccatggc ttccagtccg tgatcacacg ttgaattccc tccggtttga tccctggccc
     7081 cgttacga
//
LOCUS       DAOSOR010000291         7069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10676_length_7069_cov_4.795979, whole genome shotgun sequence.
ACCESSION   DAOSOR010000291 DAOSOR010000000
VERSION     DAOSOR010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10676_length_7069_cov_4.795979"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="PK710_15075"
     CDS             <1..925
                     /locus_tag="PK710_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11100.1"
                     /translation="TGTSLALRCLAAMGVDQIMHGQRLPKGKGVPELLFFNVQNPDAM
                     RSNIRQSAIDEVVRARLIRERGMVIPASQSATAKAIRIARSPVVFFGHSQGGLNGPLF
                     MAVDDTARGGVLSGSGSMITLTLMYKTKPDPNVSALAKMFLLGLSRDEFSEVNAFHPG
                     LSFVQMMGDPVDPIHYVPMMVQRPHQGFEPKSILQTEGVNPDFSGDNYTPPYAIEVQA
                     IATGLPWMAPAIHRVAEASYSGMQPVTIPEHGLSGNLGGGKASGVLAQWRASEVSDGH
                     FVVTQNQAARDQASQFCRNLVDFSQGRIPAP"
     gene            complement(926..1327)
                     /locus_tag="PK710_15080"
     CDS             complement(926..1327)
                     /locus_tag="PK710_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11101.1"
                     /translation="MSRNRLGLILGASLLMIAVIALGIYRLFFSCAFSEGCKESGLCT
                     TASGACIAGSVDECRKSEDCERKGRCHLSAERCVAGTDHDCRRSVWCKERGMCSLEGE
                     DCIANSDEDCRQSEKCIKHRQCVAKSGVCVM"
     gene            complement(1382..2389)
                     /locus_tag="PK710_15085"
     CDS             complement(1382..2389)
                     /locus_tag="PK710_15085"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOT11102.1"
                     /translation="MRRFLIILPLAFILAGCASDSPNESNGSSGGAAGSAGMPGSGGA
                     TQDVAQEPIPQSPGPMRLVSWNCWNLFDDVLGNCVGYCPYEDKLTTTQYKNKIAAIAK
                     TLRVLDGDIVLLQEVENIGILDTLVQHKDLADMGYQTRHLLPGNDPRGINIGLLSRVP
                     VDDYISHKSDKFTRIDNPAYVYQFARDAVEIHLTYRGHKVGIVGVHFKARINDDDPDR
                     RVAEAQQARAIADNILAADPNTYVFVMGDFNDTPGTDTYAAVRNGPTPQSLVFEHAMS
                     SIPSAERYSYVYGKDKQLIDHILASPNAGARLDPDSAVILHDATAASDHHPLAATYTV
                     P"
     gene            complement(2535..2858)
                     /locus_tag="PK710_15090"
     CDS             complement(2535..2858)
                     /locus_tag="PK710_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11103.1"
                     /translation="MTSDEQEKREKAIAAVKDAWLILAEEAPVDPGELVDVAHVRVLD
                     GEGERVDAAVAIVRKGKVSRAATQGLGDTFESACDMLAQILLLRASLLRASNGESAIS
                     DDFDD"
     gene            complement(2937..3821)
                     /locus_tag="PK710_15095"
     CDS             complement(2937..3821)
                     /locus_tag="PK710_15095"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOT11104.1"
                     /translation="MSLAQLGEIDWIRPRDLRHACEQLARIHDEGKKVWALCGCTDWM
                     VERHIAPVDQNPLDAVALDISNLPELRGIRVDGHVMTIGAAQPYAAIRRDPMVREHCP
                     MLAQMASEVGARQIQSRGTLGGNLVTGSPAADGVTALFALDATVTLQGPSHERSVPVT
                     SFYSGYRTTVRRPDELVVRVSFELPKPGAAQTWRKVGTRKAQSISKAALAAVAETEEG
                     VITRVGFGAASVAEVVLPLASVRSLVLGKNVHSLNMNDVEEAVDSEIRPIDDVRSTGR
                     YRRHLMKVLVKRFLQALA"
     gene            complement(3821..4294)
                     /locus_tag="PK710_15100"
     CDS             complement(3821..4294)
                     /locus_tag="PK710_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOT11105.1"
                     /translation="MMITIELTVNGSKQNVQASPLARLIDVLREKLRLTGTKEGCGEG
                     ECGTCTVLLDGEPVNACLVPFGQCEGRHVVTIEGLVNETNLSSLADAFIERGGAQCGI
                     CTPGMIVSSHWLLQHNPTPSDAEIREALAGNICRCTGYTGIVEAVAKASGRQGGT"
     gene            complement(4291..6531)
                     /locus_tag="PK710_15105"
     CDS             complement(4291..6531)
                     /locus_tag="PK710_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOT11106.1"
                     /translation="MQSNVRSQGWIGDTPARSDGPAKVAGTARYIADIAFPNAIDASV
                     VRSHVPRGRILGIDFDPNFDWSDVTIITAKDVPCNEIAEIECDQPALADDQVRHVYEP
                     IVLLACEDKTKLQRAVQRVHVRIEQLPALLNVDDSLACNPLVLGKDNVMCSYRVVLGD
                     AKAEIERAEVVVRGMYEVGHQEQMYIEPQGCVAYWDDAGLHVVGSMQCPYYVEKALAH
                     ALRLERSRIDVTQSVTGGAFGGKEEYPSVVAVHVALLSKKAGRPVRMIYERQEDVQAT
                     TKRHPARVTVEAGCDRDGTLRGIAVDILMDAGAYVTMTPVVLSRGVLHACGAYRWPTA
                     LVQSKAVITNTPPNGAFRGFGAPQTIFAVERHMDRMAEAIGMEPMAFRQKNLLGIGAT
                     MPTGQVLNGSVGVQECVQRALEKSDYLRKKSTLGKANSPAGATKVRGVGASVFLHGAG
                     FTGSGEDRLKGAVELDLLKDGHVRVRSSSTDMGQGTETVFRQIAAQAAGVPFENVSVA
                     TPSTAHVPDSGPTVASRTVMIVGSIVESAAKELGERVRNEQQQHGGSFAEASNRLLER
                     EGTVVVHRRYAHPAGVKFDDATYTGTAYPAYAWACDIAEVEVDLDTFEIDVVGFWSAV
                     DVGRAIHPVLCRGQLDGGALQSIGWALCEEVQWKDGHILNPRMTTYIVPTALDAPPFM
                     TDLVEEPYTFGPGGAKGIGELPMDGGAPALAAAIEHATGIAFNRIPMTPERLFDAWNE
                     SQRERR"
     gene            complement(6576..>7069)
                     /locus_tag="PK710_15110"
     CDS             complement(6576..>7069)
                     /locus_tag="PK710_15110"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOT11107.1"
                     /translation="RASVLRTIRPSLPHVRMIPSSAPGCWGPAGSIMAAARAGALAHS
                     PWTLITPVDAVPVEQPTLQRLLDSRSPRAQAVRPRFEGKGGHPVLVRTQIIMNVYLEQ
                     GPTPLRDVLRALVVVDVPMLDPRVIADLNTPEAYRAWTGREPRFEAILSDSVTIDKNG
                     GFD"
BASE COUNT         1482 a         2025 c         2026 g         1536 t
ORIGIN      
        1 gacgggcaca tcgctggcgc tacggtgttt ggcggcgatg ggagtcgatc agatcatgca
       61 cggccagcgg cttccgaaag ggaaaggggt gcctgaatta ttgtttttca atgtgcaaaa
      121 ccctgatgcg atgcgaagca atattcggca atcggccatc gatgaagtgg tcagggctcg
      181 cttgatccgc gaacgtggta tggtgatacc cgcttctcaa tcggccacgg caaaggccat
      241 acgaatcgcg cggtcgcccg tggtgttttt cggtcattct cagggtgggt tgaacggtcc
      301 gttgttcatg gcagtcgatg ataccgcgcg cggtggtgtg cttagcggtt cgggatccat
      361 gataacgctc acgttgatgt acaaaaccaa gccggatccg aatgtctcgg ccttggccaa
      421 gatgtttttg cttgggttgt cacgcgatga gttttcggag gtcaatgctt ttcatccggg
      481 gctatcgttc gtgcaaatga tgggcgatcc cgtggatccc atccactacg tgcccatgat
      541 ggtgcagcgt ccccaccagg gattcgaacc gaagagcatt cttcaaacag aaggggtcaa
      601 tccggatttt tctggagaca actacacgcc cccgtatgcg atcgaagtgc aggccattgc
      661 cacgggactt ccgtggatgg ctccggcgat tcatcgcgtg gccgaggcgt cgtattccgg
      721 aatgcagccc gtgacgatcc ccgagcacgg tttgtcgggc aatcttggtg gaggcaaagc
      781 aagcggggta ttggcacaat ggagggcgtc ggaggtgagc gacggtcatt tcgtggtgac
      841 ccagaaccag gcagcgcgcg atcaagcctc gcaattttgt cgaaatctcg tggatttttc
      901 gcaagggcgt attccagcgc cgtgatcaca tgacgcagac accggatttc gcaacgcact
      961 gacggtgttt gatgcatttt tcggattgac ggcagtcctc gtcggagttg gcgatgcagt
     1021 cttccccttc gagagagcac atgccgcgtt ctttgcacca aacggatcgt ctgcagtcgt
     1081 ggtccgttcc ggccacacaa cgctccgcgg acaggtggca tcgtcctttg cgttcacagt
     1141 cctcggactt tcggcactcg tcgaccgagc cagcgataca ggctccactt gcggtggtgc
     1201 agagcccgga ttctttgcaa ccctccgaaa atgcacaaga aaaaaagagc cggtagatac
     1261 cgagggcgat cacggcaatc atcaacaggc tggccccgag gataaggccg aggcgattac
     1321 gagacattgg catcatccta ccagctttca cggagggggt ggtggacctc gggttgggga
     1381 ctcaagggac ggtataggta gcagccaagg ggtggtggtc gctcgccgcg gtggcgtcgt
     1441 gcagaatgac agcggagtcg gggtcgagac gagcacccgc atttgggctg gcaaggatgt
     1501 ggtcgatgag ttgcttgtct tttccgtaga cgtacgaata ccgctccgcg ctgggaatgg
     1561 aagacatggc gtgttcaaag acgaggcttt ggggagttgg gccattgcgt acggcagcgt
     1621 aggtatcggt accgggtgta tcgttgaagt cgcccatgac gaagacatag gtatttgggt
     1681 ccgcagcgag gatattgtcg gcgatagctc gtgcttgttg cgcttccgcg acccgtcggt
     1741 cagggtcgtc gtcgttgatt cgggctttga agtgaacccc cacgatgccc accttatgac
     1801 ctcgatacgt caggtggatt tcgacagcgt cccgtgcgaa ttggtagacg taggcggggt
     1861 tgtcgatccg tgtgaactta tcgcttttat gagagatata gtcatcgacg ggaacccggc
     1921 tcaacaagcc gatgttgatg ccacgggggt cattgccggg caatagatgc cgcgtctggt
     1981 atcccatatc cgcgaggtct ttgtgctgaa cgagggtatc gaggatgccg atgttttcta
     2041 cttcttgcag aagcacgata tccccatcga gtacgcgtaa cgttttggcg atggcagcga
     2101 ttttgttttt gtactgcgtg gtggtgagtt tgtcctcgta agggcaatag ccgacacaat
     2161 tgccaaggac gtcgtcgaac aggttccagc aattccatga caccaggcgc atgggccctg
     2221 gtgattgagg gatgggttct tgcgcgacgt cctgggttgc gccacccgag ccgggcatcc
     2281 ctgctgagcc agcggcacct ccggaagagc cgttggattc gtttggggaa tcggacgcgc
     2341 atccagcgag gatgaaagcg agaggaagaa tgattaagaa gcgtcgcacg taggaatctt
     2401 ggactcggga gcttggagaa gcaagggaaa cgagggatac gatgcgatcg tgatcgtggg
     2461 gaaacgcggt gaagtgtagg gattcgaacg aaggggctcg tgtcgttcgg agtctgcctg
     2521 agcagaatca ggcatcaatc gtcgaagtcg tcggatatgg cactttcacc attcgaagca
     2581 cgaagcaacg aggcgcgaag gagcaagatc tgggcaagca tatcgcatgc gctttcgaaa
     2641 gtatcgccga ggccttgcgt tgctgctcga gatacttttc ctttgcgcac gatggcgacg
     2701 gcggcatcga cccgctctcc ttccccgtcc aacacgcgca catgcgccac atcgacgagt
     2761 tcaccagggt ctacgggcgc ttcttcggca aggataagcc acgcgtcctt caccgccgcg
     2821 atcgcctttt ctcgtttttc ctgctcgtcg gatgtcacgg tgcgtgccca ccttgtccag
     2881 cttgatgcag agggagactc atagcaaggt tcttcgatgg aatcgaccct ttgaggctat
     2941 gccagtgctt gtagaaatcg ttttaccaat actttcatca gatgacgacg gtatcgaccg
     3001 gtcgaacgaa catcatcgat gggacggatt tcgctgtcaa cagcttcttc cacgtcgttc
     3061 atattcaagg aatgaacgtt ttttcccaag accaaggagc gaaccgaggc gagagggagc
     3121 acaacttccg ccacggaagc ggcaccgaag ccgacacggg tgatgacccc ttcttcggtt
     3181 tccgccacgg cggcgagggc ggctttggag atcgattgcg cctttcgcgt gccgactttt
     3241 cgccaggttt gcgcggctcc aggtttggga agttcgaaag aaacgcgcac caccagctcg
     3301 tcgggacgtc ggacggtcgt ccgatatccg gaataaaacg aagtgacagg aaccgagcgc
     3361 tcgtgggagg ggccctgcag ggtcacggtt gcatccagag caaatagcgc ggttacgcca
     3421 tcggccgcgg gtgaacccgt caccaggtta cctcccaggg tgcctcggct ttggatttgt
     3481 cgcgccccca cttcggaagc catctgcgcc aacatcggac aatgctcgcg aaccatcgga
     3541 tcgcgccgta tcgctgcgta gggctgggca gcgccgatgg tcatcacgtg gccatctacc
     3601 cgaattccac gtaattccgg caggttagag atatccaggg ccaccgcgtc gaggggattt
     3661 tgatcgaccg gcgcaatatg ccgttcgacc atccaatccg tacacccgca aagagcccat
     3721 actttttttc cctcgtcgtg gattcgtgcc aactgctcgc aagcgtgccg caggtcccgc
     3781 ggacgaatcc aatcgatctc gccaagttgc gcaagactca tcacgtgcct ccttgccgtc
     3841 ccgacgcctt ggccacggcc tcgacgatgc ccgtataccc tgtgcatcga cagatgttgc
     3901 ccgcgagggc ttcgcggatt tcagcatccg atggcgttgg attgtgctgc aacagccaat
     3961 gcgacgatac gatcatgccg ggggtgcaaa tgccgcattg cgcgcctccc cgttcgatga
     4021 acgcgtcagc gagcgatgat agattggttt cattcaccaa tccttcgatg gtcaccacgt
     4081 gacgaccttc acattggccg aagggcacca agcaagcatt gacgggttct ccgtccagaa
     4141 gaaccgtaca cgttccgcat tctccttctc cgcacccctc cttggtgccg gtaagccgga
     4201 gtttttctcg caaaacgtcg atcaaacgag cgaggggcga agcttgtacg ttttgcttac
     4261 tcccgttgac ggtcagttcg atcgttatca tcatcgcctc tcccgttgcg actcattcca
     4321 ggcgtcaaat agtctttcgg gggtcatggg aatgcggttg aacgcgatgc cagtggcatg
     4381 ttcgatggcg gcggcaagcg cgggagcgcc accgtccatc ggcaactcac cgattccttt
     4441 ggctccccct ggaccaaacg tatagggttc ttccaccaga tccgtcatga atggcggagc
     4501 atccagggcg gtgggcacga tataggtcgt cattcttggg ttgagaatat gcccgtcttt
     4561 ccactgcact tcctcacaca aggcccaacc aatcgattgc aacgcgcctc cgtcgagttg
     4621 gcctctgcac aagacaggat ggatcgcgcg tcccacatcg acggcagacc agaagcccac
     4681 aacatcgatc tcgaacgtat cgaggtccac ctctacttcc gcgatatcac aagcccatgc
     4741 gtacgcggga taagccgtgc ctgtataggt tgcatcgtcg aacttgaccc cggcaggatg
     4801 cgcatatcgt cgatgcacca ccacggttcc ttcccgttcg agcaaacggt tcgaagcttc
     4861 ggcgaaagac ccaccgtgtt gctgttgctc gttacgaacc cgttcgccaa gttctttggc
     4921 ggcgctttcc acaatggatc caacgatcat gaccgtgcgg ctggcaaccg tgggtcccga
     4981 gtcgggcaca tgagctgtcg atggggtcgc cacggaaaca ttttcgaatg gaacgcccgc
     5041 cgcttgcgct gcaatctgcc gaaatacggt ttccgttcct tgtcccatat ccgtggaact
     5101 cgaacgcacg cgcacatggc cgtctttcaa caaatcgagt tcgaccgcac ctttgagacg
     5161 atcttcacca gagccagtaa accccgcgcc atgaaggaag acgcttgctc ctacgccgcg
     5221 cactttggtg gcgccagcag gggaattcgc cttccccaac gtgctttttt tccgcaggta
     5281 atcgcttttt tcgagggctc gttgcacgca ctcctgcacg ccaacacttc cgttcaacac
     5341 ctgtcctgtc ggcatcgtag cgccaatgcc gagcaagttc ttttgtcgaa acgccatcgg
     5401 ttccatcccg atcgcttccg ccattcgatc catgtgccgt tcgactgcaa agatcgtctg
     5461 cggcgcccca aatccacgaa atgccccatt gggaggcgtg ttggtgatga ccgccttgga
     5521 ttgcacaagc gccgtgggcc atcgataagc gccacaagca tgaagcacac cgcgcgacaa
     5581 caccaccggc gtcatcgtca cgtacgctcc cgcatccatc aagatatcga ccgcaatgcc
     5641 gcgaagcgta ccatcgcgat cacatccagc ttccacggtc acacgcgccg gatgccgttt
     5701 cgtcgtggct tgcacgtctt cttgccgttc gtaaatcatt cgaacaggac gcccggcttt
     5761 tttcgacaaa agcgcgacat gcaccgcaac caccgacgga tattcttctt tgccgccaaa
     5821 agcgccgccg gtcaccgatt gggtcacgtc aatgcgagac cgctcgagtc gcagcgcatg
     5881 cgcaagcgct ttctcaacat aataagggca ttgcatcgac ccgacgacgt gcaagccagc
     5941 gtcgtcccaa tacgccacac agccctgtgg ttcgatatac atctgctcct ggtgacccac
     6001 ttcgtacatt ccacgaacca cgacttcagc tcgttcgatc tcggctttcg catctccaag
     6061 cacgacgcga tagctacaca tcacgttgtc ttttccgagc acgagaggat tgcacgccaa
     6121 cgaatcgtcc acattgagca gcgcaggaag ttgttcgatt cgaacatgga cccgttggac
     6181 agcccgctgg agtttagtct tatcctcaca agccagcaga acgatgggtt catagacatg
     6241 gcgaacttga tcatccgcta gcgcgggttg atcacattca atctccgcga tttcgttaca
     6301 gggaacatct tttgcggtga tgatggtcac gtcggaccaa tcgaaattcg gatcgaaatc
     6361 gatacccaag atgcgtcccc gtgggacatg actgcgaacg acagaggcgt cgatcgcgtt
     6421 gggaaacgca atatccgcga tgtatcgggc cgtaccggct actttcgcag ggccatcgga
     6481 acgcgcggga gtgtctccga tccagccttg gctcctaacg ttggactgca tggtagcaat
     6541 ctagcagcgc cacccacgaa tggagccgaa tttcctcaat cgaatccacc gtttttgtcg
     6601 atagtgacag aatcggaaag gattgcctcg aaccgaggct cccgccccgt ccacgcgcga
     6661 tacgcctctg gggtattcaa atccgcgata acccttggat caagcatcgg aacatccacg
     6721 accacaagcg ctctcaacac gtcgcgcaag ggcgtgggac cttgttcgag atagacgttc
     6781 atgatgatct gcgttcgcac cagcacggga tgcccgcctt ttccctcgaa tcgtggtcga
     6841 acggcttgcg cccggggact tcgggaatcg agcaatcgtt gcagcgttgg ttgttcgacg
     6901 ggaacagcat ccactggcgt gatcaacgtc catggcgaat gggccaatgc gcctgctctt
     6961 gccgcggcca tgatggatcc cgcgggtccc cagcagcctg gcgcgctcga tggaatcatc
     7021 cgcacatggg gaagcgaggg acgaatcgtc cgaaggacgg aagcacggg
//
LOCUS       DAOSOR010000292         7063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10693_length_7063_cov_5.142123, whole genome shotgun sequence.
ACCESSION   DAOSOR010000292 DAOSOR010000000
VERSION     DAOSOR010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7063
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10693_length_7063_cov_5.142123"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..1505
                     /locus_tag="PK710_15115"
     CDS             162..1505
                     /locus_tag="PK710_15115"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT11108.1"
                     /translation="MEYHGGTESNRPTAHGIRKVVDEIIIQRTKKNRIWRLHVNRMPL
                     IGSTIDHRYQLYRELAVGGMATIYEAKHLYTERRFAIKILHKNNATNRARLLREASAL
                     QGERHHSLIDVTDAGESPEVGPYLVMELLKGRSLEAMLAARQTLYGDEVVQIGLELCD
                     ALAFVHQRGMIHRDIKPGNIFLIDEKPYKIKLIDFGIVSTPFGEKLTKESAAPGTPEY
                     MSPEHIRQESIDRRSDIYSLGVTLFECLTGSVPVSGTYNSIFVHFSKPCSITKVRDIR
                     SDTPLELATVIERAIAPVLDDRFQDMREFSNALREITNLPSRREAHTTPQRRVFRRAP
                     YGTPVRLIRSNGNRIDGRSEDISEGGMLLLVECPLNVGETIHVRFASPVVGSIVTVSS
                     IVRWVRTSRGGKTVLGCSFNEISGDLRRDISRYVEWMSDPNSAPTDTNNLLVSST"
     gene            complement(1759..2901)
                     /locus_tag="PK710_15120"
     CDS             complement(1759..2901)
                     /locus_tag="PK710_15120"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOT11109.1"
                     /translation="MVRRLCLGCEKSALALVLLAVLAPSRAWAQDASLDEARTHFEEG
                     VARYEKNDFLGALEAFAQADRRKHVPAITYNIARARESLGQVQDAIDAYESYLAEAGA
                     EGDYLAAATVALTTLKARASKLRVETTPPGANVRIDGRIWREKTPTTIWVSRGYHRVD
                     VELQEWRAGADIDAEGNGKEESVVLQRPSAPDRPAAAPKRLAPTYDGLVAGLGLSLNY
                     AALVVKAERPSRENDDGSYQTTSLRFGLVLEVGYALSPRSVVSVKGDCGLGSTEKALF
                     SLGLGSVVYAWRATEHWWISAGGIMGSSDELLGATYTPLFGPREDKSIVLGSKLAIGP
                     VVGTSVAIGRDGTGEWTMGIHPSLLLGTGQEQSTLFVPVVVGRRWF"
     gene            complement(2904..4409)
                     /locus_tag="PK710_15125"
     CDS             complement(2904..4409)
                     /locus_tag="PK710_15125"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT11110.1"
                     /translation="MSFADQEDQWPEGVPRRGEVVGDKYVCEQLLGKGGMGAVLLARH
                     NQLGQPVAIKVLLPSTLKNPLAQERFLREARSVIALRSDHVARVIDFGTLPSGCPFLV
                     MEHLEGQDLKALITRDGPLPIDTAVDFVLQACEALCEAHQQGIVHRDLKPANLFLSRR
                     VDGSPLVKVLDFGISKTVASATASSGSGPSITNTGAVFGTPAYMSPEQLRNSKHVDHR
                     ADVWSLGVCLFEIITARLPFGSEEDGIASMCAHILEDSPAALHRLRDDAPAGLEAILL
                     RSMQKNPAERFPSIAAFAEAMAPFASSNSTEVLLRIGRMASNSLPFETTLLSDPPKLS
                     GHPPSSGWGRTGSQSSGKKEVIVGLAVAAVLLAGVGIGWTWWTHSQRPLVGVTVPTLP
                     SKASSSAVVSVANVHGSSPSDVPSVPSARPLTSASATSPLATKPPPSASAKPSATVKR
                     SASAHAAPAESAPPPALPSCPAGQAVSNGHCCAIGFEWKGGACVPGVAKGF"
     gene            complement(4516..5277)
                     /gene="lipB"
                     /locus_tag="PK710_15130"
     CDS             complement(4516..5277)
                     /gene="lipB"
                     /locus_tag="PK710_15130"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOT11111.1"
                     /translation="MITRSIRVYWLGLRSYGSVHELQQQALVARQQGLMGDIVLLLEH
                     RPVITLGRSAKNEHVLVSQEALASRGIDLVTTARGGDVTYHGPGQLVAYPIVSLEPDR
                     CDVRRYVRDLLETMAGLANEHGVSAGIIDEYPGIWVDTANPRHWPGQNHAVRPAKLGA
                     VGVRISRWVTMHGFAFNASTDRSGFAAIIPCGIRQYDVTTLDELVGGKPQPQALAPRA
                     AELFCERFGADLESYSHVDADDAQMADAIGIPATC"
     gene            complement(5358..6830)
                     /locus_tag="PK710_15135"
     CDS             complement(5358..6830)
                     /locus_tag="PK710_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT11112.1"
                     /translation="MSNPLELEDRPRVLVVDDEKFIRDILADFLGLEGYFVRTAPDGV
                     SALDVLHASRFDVVISDLKMPRMGGIELLEAIPRISPNTLTIIMTGFGTVETAIEAMK
                     RGAYDYVLKPFKMDEVIHIVRRGIDKQRLAAENLRLKEAVSLYSVSEAISASLSLEEI
                     LETIGQLAVQEMKADVVTIWLEDRKGGFVERHSRMGNRLQFGGQLHIDVIVQHLMNDQ
                     NIVAHGTSSERYFASTPDSPIVSLLVVPLKVTRRLLGWIALVSTNHTVRFDEGHRKLF
                     SIIGARAAAAIENAKLYEELQGTFQQTIHGLAKAIDKMDRYTSGHSDRVALYATFLAN
                     KLGLSEDVVAVVNQSALMHDIGKIGCVLNLNKPGTLTQEEYEVFKKHPQYGKDILEPI
                     KFLHPLIPGVQLHHERWDGKGYPLGLRGTEIPLIARIIAVADAYDAMTSNRAYRRALP
                     QDVAIGEIERCAGTQFDPEAALAFIEGIDEFMANRKKLRG"
BASE COUNT         1676 a         2004 c         1878 g         1505 t
ORIGIN      
        1 gattacaacg taatctggcg cttgttcctc tcccgtttgc gaaatctacg ctcaaaaagg
       61 cgaggtgcta agattcgagg aatggcatgg ggtacttata tgcggcgaga gggacccgtg
      121 tcggtcgctt ccagacccat cgtgaatgat ttggggaccg catggagtac catggtggaa
      181 cagaaagcaa ccgtccaaca gcacatggaa tacgtaaagt agttgacgaa ataattatcc
      241 agcgaacaaa aaaaaaccga atctggaggc ttcacgtgaa caggatgccg ctcattggaa
      301 gcacaatcga tcaccgttat cagctttatc gtgaacttgc cgttggaggt atggcaacca
      361 tttatgaagc taagcatctg tatacagaac gtcgtttcgc cattaaaata cttcataaaa
      421 acaacgctac caacagggcg cgcttgcttc gagaagcttc ggctcttcaa ggggaacggc
      481 atcattctct catcgatgta accgatgctg gggaatcccc agaagtaggt ccctatcttg
      541 tcatggaatt gctaaaagga agaagcctcg aagcgatgct ggcggctcgt caaaccctat
      601 acggcgatga agtcgtacaa attggattgg aactctgcga tgctctcgct ttcgttcatc
      661 aacgcggaat gattcaccgc gatataaaac caggtaatat ctttctgatt gatgaaaaac
      721 cttataaaat caagctaatc gatttcggga tcgtatcgac cccatttgga gaaaagctta
      781 ccaaagaatc cgcagctccc ggtactcccg aatatatgtc acctgaacat atccgacaag
      841 aatcgatcga tcggcgaagc gatatttatt ctcttggtgt tacgctcttc gaatgcctca
      901 ccggttcggt gccggttagt gggacataca attctatttt tgtccatttt tctaaaccat
      961 gctcaattac aaaagtcagg gatataagat ctgatacacc attagaactt gcgactgtta
     1021 ttgaacgagc aattgctcct gttctggatg atcgtttcca agacatgcgt gaattcagca
     1081 atgcgcttcg tgaaataacg aatcttccat ctcgtcgaga agcgcatacc acacctcaac
     1141 gaagagtgtt tcggcgagca ccctacggaa cccccgttcg acttattcgt tcgaatggga
     1201 atagaattga tgggcgcagc gaagatatct cggaaggtgg catgttattg ctggttgaat
     1261 gtccgttgaa cgtcggagaa accatccatg ttcgtttcgc atcaccggtc gtgggttcta
     1321 ttgttacagt atccagtatc gtgcgatggg tccggacctc acgaggtggc aaaactgtcc
     1381 ttggatgttc gttcaatgaa atctcagggg atctccgacg cgatatttcc cgctatgttg
     1441 aatggatgag cgatcccaat tcagcaccta cggacacaaa caatttgttg gtgtcttcta
     1501 cctagccata cgatcgtcgt gaaaatgacg cattagtcct aaaaatccag tatagaaata
     1561 ctctacccac aactcatcca aattcctaaa tataccgttc gatggcgaac tcacgcaccc
     1621 tgctatcgct acccgccatc cgtacccttc gacaaagact agaagcattt caataaagag
     1681 aaatcagata ccttcaaacc aatagcatac gaggggctgt cacctcgacg cgatcaacag
     1741 caatgtcgtc gccaaagttc aaaaccaacg acggccaacc accacgggta cgaaaagagt
     1801 cgactgctct tgcccggtcc ccaaaagcaa gctcgggtga atacccatgg tccattcgcc
     1861 agtgccatcc cgaccgatgg ccaccgacgt gcccacgaca ggaccgatgg ctagcttgct
     1921 ccccaaaaca atggacttgt cttcgcgggg gccgaagagt ggtgtgtagg tggcacccaa
     1981 caactcatcg ctggaaccca tgataccccc agccgaaatc caccaatgct cggtggcacg
     2041 ccaggcgtag acgacactac ctaacccaag ggaaaataaa gctttttcgg tggatcccag
     2101 cccgcaatcg cctttgacgc tcaccacgga acgtggcgaa agcgcataac caacctcgag
     2161 aacgagcccg aagcgcaagg acgtggtctg atacgaacca tcgtcgtttt cgcgcgaagg
     2221 ccgttcggct ttgacgacca atgccgcata attcaacgac agccccaacc cagccaccaa
     2281 gccgtcgtac gtgggcgcca ggcgtttggg cgcagcggca ggacgatccg gagctgaagg
     2341 gcgttgcaac acgaccgact cctctttgcc gtttccctcg gcatcgatat ccgctcctgc
     2401 ccgccattct tgcagctcca catccacccg gtgataaccg cgcgaaaccc atattgtcgt
     2461 aggggtcttc tctctccaaa ttcttccatc gatccgaaca tttgcgcccg gaggggtggt
     2521 ttcgacgcgc agtttgctcg cccgtgcttt caacgtggtg agggccacgg tggccgcggc
     2581 tagataatct ccctcggctc ccgcttccgc gaggtaggat tcgtaggcgt caatggcgtc
     2641 ctggacctgt ccaagactct ccctggctcg cgcaatattg tacgtaatgg cagggacatg
     2701 ctttcgtcgg tccgcctgtg caaaggcttc cagcgctccc aggaaatcgt tcttttcata
     2761 gcgagccacc ccttcttcga agtgcgtgcg agcttcatcc agggaagcgt cttgcgccca
     2821 cgccctcgac ggagccaaga cagcgagaag gacgagcgcc aaagcgcttt tctcacaccc
     2881 caggcatagc cttcgaacca tggttaaaac cctttggcca cgccaggcac gcacgcgcca
     2941 cctttccatt cgaaaccgat ggcgcaacaa tgaccgttcg atacagcctg cccagcagga
     3001 caagagggta aagccggagg tggagcactt tcggcaggcg cggcatgagc gctggcagag
     3061 cgtttgaccg tggcagacgg ttttgcgctc gcggaaggtg gcggcttggt cgcaagcggg
     3121 cttgtcgcgc tcgcggaagt caatgggcgc gcagaaggga cgcttggaac atcggagggc
     3181 gatgaaccgt ggacgtttgc caccgacacg acggcggagg atgaggcttt ggaaggaagg
     3241 gtcggaacgg taacgccgac gaggggtcgt tgggagtggg tccaccaagt ccatccgata
     3301 ccgacccccg cgagcagcac ggcagccacg gcaagcccca cgatgacctc tttttttcca
     3361 gaggattggg aaccggtgcg cccccagccg ctcgaaggag gatgacctga gagctttgga
     3421 gggtcgctca gcaaggtggt ttcgaaagga aggctgttgg atgccatcct tccgatacgc
     3481 aaaagcactt ccgtgctgtt cgaagaggcg aagggagcca tggcttcggc aaaagccgcg
     3541 atggaaggga atcgctcggc ggggtttttc tgcatgctcc gcaacaaaat cgcctccaaa
     3601 cccgcggggg cgtcgtcccg caaccgatgc aaagcagcgg gagaatcttc gaggatatgg
     3661 gcacacatcg aggcaatccc atcctcctca ctaccgaaag gaagacgagc cgtgatgatt
     3721 tcgaaaaggc aaacgcccaa agaccatacg tcggcccggt gatcgacgtg tttcgaattt
     3781 cgtaattgtt ccggggacat atacgcgggc gtaccgaata cggcaccggt attggtgatg
     3841 ctggggccgg acccactgga agcggtggca gaggcaacgg tttttgagat tccgaagtcc
     3901 aacaccttga cgaggggaga gccgtccacg cgtcgagaga gaaagaggtt ggcgggtttg
     3961 agatcccgat ggacgatacc ttgttgatgc gcttcgcaca gggcttcaca agcttgcaac
     4021 acgaagtcca cggcggtgtc gatgggcaaa gggccgtcgc gcgtgatcag ggctttcaga
     4081 tcctgtcctt ccagatgttc catgacaagg aaagggcaac cggaaggaag ggttccgaag
     4141 tcgatgacgc gggccacatg gtcactgcgc aaagcgatga ccgagcgcgc ttctcgcagg
     4201 aaacgctctt gcgcgagagg gtttttcagg gtcgacggca gcagtacttt gatggcgaca
     4261 ggttgcccga gctggttgtg acgagccaac aggacggcgc ccattccccc ttttccgagc
     4321 agttgctcgc atacatactt atcccccacg acttcgccgc gccgcggtac gccttccggc
     4381 cattggtctt cctgatcagc aaaggacatg gggcagcaaa ccgtatccga tatcgaactt
     4441 acgggatagg aagcctatcc ggccaaaggc ggcatcgcaa cgggaacccg cggtaggcat
     4501 ccaccgaacg gctcttcaac acgtcgcagg tattccgatg gcatcggcca tctgtgcatc
     4561 gtcggcgtcc acatgcgaat agctctcgag gtcggcacca aatcgttcgc aaaacagctc
     4621 cgcggcacgg ggcgcgagcg cttgtggttg aggttttccc cccaccaact catcaagggt
     4681 agtcacgtca tactgcctga ttccacaagg gatgatggca gcaaagccgc ttcgatccgt
     4741 actggcgttg aaggcgaaac catgcatggt cacccaacgg gagatacgca ctcccaccgc
     4801 tccaagtttc gcaggtcgaa cggcatggtt ttgtccgggc caatggcggg gattcgccgt
     4861 atccacccag attcccgggt attcatcgat gatgcccgcc gacacaccat gctcgttggc
     4921 caagcccgcc atcgtttcca gcaagtcccg cacataacga cgaacatcgc atcggtccgg
     4981 ctccaacgat acgatcggat aagccacgag ttgccccggc ccgtgatacg tgacatcgcc
     5041 gcccctggcc gtggtgacga ggtcgatgcc acgggacgcg agcgcctctt gagatacaag
     5101 cacgtgttcg tttttggcac ttcgtccaag agtaatgacc gggcgatgct cgagcagcag
     5161 gacgatgtca cccatcaacc cttgttgacg cgcgacaagg gcctgctgtt gcaattcgtg
     5221 tacggaacca tagctccgaa ggccaagcca ataaactcga atgcttcggg tgatcacgtc
     5281 gggtgctctc tcgtcgttat aaggggtatg ccaagacaat ccatatacgg ataggcatgg
     5341 aacaagggca ggacgactca gccgcgaagt ttttttcgat tcgccatgaa ctcatcgatt
     5401 ccttcgatga aggcaagtgc tgcctcggga tcgaactggg ttcccgcaca ccgctcgatc
     5461 tcaccaatcg ccacgtcttg cggcaatgcc cttcgatacg cgcgattgct ggtcatggca
     5521 tcataggcat cggcgacggc gatgatgcgg gcgatgaggg gaatttccgt tcctcgaagc
     5581 cccaggggat atcctttgcc atcccatcgc tcgtggtgga gctgaacccc tggaatgagc
     5641 ggatgcaaga acttgatggg ctccaagatg tcttttccgt attgtgggtg ctttttgaat
     5701 acttcgtatt cttcttgtgt cagcgtgccg ggcttgttca agttgagcac acacccaatt
     5761 tttccaatgt catgcatgag cgcggactgg ttcaccaccg caaccacgtc ttcggatagc
     5821 ccgagtttat ttgccaaaaa cgttgcgtat aaggctaccc gatcggaatg acccgaggtg
     5881 tatcgatcca tcttgtcgat ggctttggcg agaccgtgaa tggtctgctg gaacgttccc
     5941 tgcaattcct catagagttt cgcattttcg atggcggctg cagcccgcgc accgatgatc
     6001 gaaaatagct tgcgatgccc ttcatcgaat cgcacggtgt gattggtcga cacaagagcg
     6061 atccatccaa gaagtcgtcg cgtcaccttg aggggaacca cgagcaaact gacgattggg
     6121 ctgtctggag tcgaggcaaa atatcgttct gatgacgtac catgagccac gatgttctgg
     6181 tcgttcatca aatgttggac aatgacgtcg atgtgtagct ggccgccgaa ttgcaatcgg
     6241 tttcccatcc gcgaatgacg ctcgacaaat ccgcctttcc tgtcctccag ccagatcgtc
     6301 acgacatcgg ctttcatctc ctggacagcc aattgtccaa tggtttcaag gatttcctct
     6361 aaggaaagac tggccgagat cgcttcactg accgagtaga gcgacaccgc ctctttcaag
     6421 cgcaaattct ccgccgcgag cctttgcttg tcgatccccc gccgcacgat gtggatgact
     6481 tcatccatct tgaacggctt caggacgtaa tcgtaagccc cacgcttcat cgcttcgatc
     6541 gcggtctcca cggttccaaa tcccgtcatg atgatcgtta gcgtattggg acttatcctc
     6601 gggatggcct ccaacaactc gatccccccc atgcgcggca ttttcagatc gctgatcacc
     6661 acgtcaaaac gcgaagcatg caacacgtcc agcgccgaaa caccatccgg cgccgttcgg
     6721 acgaagtatc cttcaagccc aaggaagtca gcgaggatgt cgcggataaa cttctcgtca
     6781 tcaacgacga gaactcgcgg gcgatcttcc agctcgagtg gattggacat ggaccgaggc
     6841 cctcagctta gcgacaatcg cgcgttgggt cgaccgtttc tacgatagcc cccggaacag
     6901 cgtcggtggc taccctgctc agccccgaca accgattcgc cgatgccaca accctttcac
     6961 cccatcctca aagctcgctc gagggcttgc caaagctgct gatcgactgc cgaccaccga
     7021 gtccccgaac gccgaagaag caaaaaatag gactcaaaca gcg
//
LOCUS       DAOSOR010000293         7045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10729_length_7045_cov_4.798283, whole genome shotgun sequence.
ACCESSION   DAOSOR010000293 DAOSOR010000000
VERSION     DAOSOR010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10729_length_7045_cov_4.798283"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2266)
                     /locus_tag="PK710_15140"
     CDS             complement(<1..2266)
                     /locus_tag="PK710_15140"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOT11113.1"
                     /translation="MRAAFLPFVVTAFCFASETAAGDLPAQIAAHWQNADSPTDNDYV
                     TLHAFVPSPTDARAQGLFPIAPGFASIRLRRDRVDTFLASHPTLPLGWSPALRPQLDR
                     LTDWTRASTFRNTTGRDGSGVVVGIVDTGVDAMHPDLRKQDGSTRIAWMLDLSRQATG
                     KHPDLEQRFGCHDLETGGCAILDAADIDQAIQSLVGTDEPRDTLGHGTHVASIAAGNG
                     LGSAEQRFAGVAPGATLVIVNATRQSTEEISDADALLATTFVFDRADAMGQPAVVNMS
                     LGVDFGPHDGTSWLEQGLASFVGAEHPGHAIVVAAGNSGGLYLSGQYTFGIHTETRVI
                     RHTRVRVPMQAISPAAATLRGTVYVWLTWREGDALSVGIEGPEGDVWIDPISPGNTAY
                     YKTTSAGKNVETIILNEYRGKESPISDATRGAVLLVHGGWSTHGPITLLLEGDGTAEL
                     WLQGVGEAGLSQNGLGMMFPRAVKHGTVSVPATHPAIIAVGASLNRLQWRNSNNQQVQ
                     IEKFGSQTPPIEDSIAFFSGSGPSSTGILKPDISAPGAFVAAAMSRDAAPSVNPFSMF
                     ANDPSTCPTASVPCMVVTPYHAIASGTSMAAPAVAGALALLLSVDPTLTAPELLTLLQ
                     AGARWPEGSVPFPFQMGPGVLDVAGALEAYELLGQPRLREPDTSKSWFVLANAYMRPD
                     PRQPVCGLIETRTSDGSVADGFDAGLLSLRVDGANVSRPLTRQAPGLWTFCVAAPTDS
                     GGGTAGIEVAYQGEP"
     gene            2449..3987
                     /locus_tag="PK710_15145"
     CDS             2449..3987
                     /locus_tag="PK710_15145"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT11114.1"
                     /translation="MRLLIRISLLFLLAGLHASCRPALPSVGRGAATSESPQEPWDLS
                     SAPSVEGATSRGSEVVPTFSPKQSAPGGKLTVGMAVDLAMRNDPRTQATWFRARAAAA
                     RLGTSEAGYYPRLDVGATGSYNHQTLQGGRMSLTQGTIGPYAKLSWLLLDFGARSANE
                     QAELAAVIAANGAHGAALQDQVLFVVDAYYRCVSAEALVEAAKADEKTATSSLEAATA
                     LHGSGKAALGDVLQSRTSLSRARMAVSTAQGQARVMRSALAVQLGMTPDVQFELEPLA
                     DDVHAQDVTEGAEQLLGKALRSRPDLVAIKAAAAASWQRAESVKHRGMPTLSLDANAS
                     RGYYLPQTYGTHGDNWGVSLTFNIPIFAGFSDSYEQQRAQWEAMAAEADARALERRIA
                     LDVWTAWLRLRTAAEQVDEASALLKISMEWEQLARGRYEAGVGTILDLMAAQSSLASA
                     RSQHVKARAEWILARVQLAHDAGELGRRTLTDVQQSVLEGGSVPSTPTAAPRGSSLAP
                     PISR"
     gene            3990..5183
                     /locus_tag="PK710_15150"
     CDS             3990..5183
                     /locus_tag="PK710_15150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT11115.1"
                     /translation="MDHLPRRFSPGFSFWPIVFLWLVLLLPACRDSQGQQQGRSSRPV
                     PVLVEKVVRKTMPVDIKATGSIEAYSVVSVQAQVGGQLQKVGFNEGDMVRKDQVLFVL
                     DRRPFDAAMRELVARRDRNQVLIDNAKRELARYDSLLEKDYVSKEDYDRAKANVDALE
                     ASLRADRSAVDAASINLQYATIRSPIEGRTGSLLVQPGNVVKPNDKALVVIRQVKPIF
                     ARFSVPEQYVASIRQRMREGKPSVTAHVRDGSPEPEHGELTMLENTVDSATGTIELKA
                     LFANELERLWPGQYVDVVLQLRVQQDAIVVPASAIQQSREGEFVYVVSAEGVASVRKV
                     VVNRSVGQESVIESGLSPDEVVVTDGQLQLTSGSKVEIKTGSPTRAVVPSGSASAAPV
                     GSSSP"
     gene            5180..>7045
                     /locus_tag="PK710_15155"
     CDS             5180..>7045
                     /locus_tag="PK710_15155"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOT11116.1"
                     /translation="MNVSAPFVRRPVMTTLVTFTLLLIGLIAYPLLPVSPLPNVDFPT
                     IVVMASLPGASPETMAASVATPLEKEFSTIDGVDSMTSTSSLGNTQITLQFDLRRELD
                     GAAQDVQAAMSRAARQLPRDMPSPPSYRKVNPAEEPIIRISLSSATLPLHVVNEYAET
                     MLAQRLSMLRGVAQVQVYGSQKYAVRVQVDPRKLKARGIGIDEVARAIQSSNVNMPTG
                     TLYGPSKAYTIDVDGQLTRAKDYGPIIVSYANGSPVRVEQLGKVLDGVENDKVAAWLT
                     NDRAIVLAVQKQPGANTVEVSKSIREVLPSFEADLPAAVNLKLLYDRSESVRESVNDV
                     QLTLLITLGLVIGVIFVFLRNVRATIIPAVAMPLSILGTFAAMYLLGYSLNNLSLMAL
                     TLSVGFVVDDAIVMLENVVRHMEMGKKRFEAALEGSKEIAFTIVSMTLSLAAVFIPIL
                     LMGGIVGRLFREFAVTITVAILVSGFVSLTLSPMMASRILGENGGKKGKAYAFTERVF
                     DAIVAWYGRTLHVVLRHRRLTMVFSLLVLGATGYLFQRVPKGFLPTEDTGQLFALTEA
                     AEGISFEAMVELQRKVAKIVSESPHVEAFMSSAGNRGGSLGSNTGMLFINLKPRRE"
BASE COUNT         1415 a         1933 c         2221 g         1476 t
ORIGIN      
        1 taggttcgcc ttgatacgcc acctcgatgc ccgccgttcc gccgccggag tccgtgggcg
       61 ctgccacaca aaatgtccac aacccgggag cttggcgcgt gagcggacgc gacacgttgg
      121 cgccatcgac acgaaggctc aacagcccgg cgtcgaaacc atccgccacg cttccatcgc
      181 tggtccttgt ttcgatcaac ccacacaccg gttgcctggg atcgggtctc atatacgcgt
      241 ttgccaagac aaaccagctt ttggacgtgt cgggttctcg aagccgcggc tgtccgagca
      301 gttcgtaggc ttccagcgct ccggccacgt ccaacacgcc aggacccatt tgaaacggaa
      361 aaggaacgct gccttcgggc catcgtgcgc ccgcctgcag caacgtcagc aactcgggcg
      421 cagtcagggt cgggtctaca gatagcagca aagcaagggc tccggcgacc gcgggagctg
      481 ccatggacgt acctgacgca atggcgtgat agggcgtgac gaccatgcat ggcaccgacg
      541 cggtggggca ggtagatggg tcattggcaa acatgctgaa cgggttgacc gagggagcgg
      601 catcgcggct catggcagcg gcgacgaaag ctcctggagc ggagatatct ggtttgagga
      661 ttccggtgga agagggacct gaccctgaaa aaaatgcgat gctgtcttcg atgggcgggg
      721 tttgggaacc aaatttttcg atttggacct gttgattgtt ggagttgcgc cactgaaggc
      781 gattgagact agctcccacc gcgatgatgg cgggatgggt tgccggcacg ctgacggttc
      841 cgtgcttgac agctcgcggg aacatcatgc caagaccatt ttgggatagt ccggcttccc
      901 cgacaccttg gagccaaagc tccgccgtac catcgccttc gagcaagagg gtgatggggc
      961 cgtgcgtgct ccaacctcca tgcaccaaaa gaacggcgcc tcgcgtggca tcggaaatgg
     1021 gactctcttt gcctcgatat tcgttcagaa tgatggtttc gacatttttc ccagcagaag
     1081 tcgtcttgta ataggctgta tttccaggcg aaataggatc gatccagacg tccccctcgg
     1141 gtccttcgat acccaccgaa agtgcatcac cttcgcgcca ggtcaaccac acgtacacgg
     1201 tgcctcgtag ggtggccgca gccggtgaga tggcttgcat gggaacccgc acccgcgtat
     1261 gccgaatcac gcgcgtttcc gtgtggattc caaaggtgta ttgtccggat aggtatagtc
     1321 ctccgctatt tccggcggct accacgattg cgtgtccggg gtgctcggcc cccacgaacg
     1381 aggcgagtcc ttgctcgagc caagaggtcc catcgtgagg tccaaagtcc actccaaggc
     1441 tcatgttcac gacggctggc tggcccatgg catcggcgcg atcgaaaacg aaagttgtgg
     1501 cgagcaacgc atccgcatcg cttatctctt cggtgctttg cctcgtggcg ttgacgatga
     1561 cgagcgtggc accaggagca actcccgcga accgttgttc ggcggagcca agtccgttgc
     1621 ccgcggcgat ggaggccaca tgggttccat gccccagggt atcccgaggt tcatcggtgc
     1681 ccacgagcga ttggatggct tggtcgatat ccgcggcgtc gaggatggca caaccaccgg
     1741 tttcgaggtc atggcatccg aaacgctgct ctaggtccgg gtgtttgccg gtggcttgtc
     1801 gcgacaagtc gagcatccac gcgatgcggg tggagccgtc ttgcttgcga agatcaggat
     1861 gcatggcatc gacacccgtg tcgacgatac cgacgacgac tccagaaccg tcgcgtcccg
     1921 tggtgtttcg aaatgtcgag gcccgggtcc agtcggtgag tcggtcgagt tgtgggcgaa
     1981 gggcagggga ccagcccaac ggcagtgttg gatgcgacgc gaggaacgta tcgactcgat
     2041 cccgacgcag acgaatgctt gcgaaacccg gtgcaatggg aaatagacct tgtgcgcgcg
     2101 catccgtggg gcttggcacg aaggcatgca gtgtgacgta atcgttgtcg gtcggtgagt
     2161 cggcgttctg ccaatgcgca gcgatctgtg ctggtagatc accagccgcg gtttcggatg
     2221 cgaaacaaaa tgcggtgacg acgaacggca agaaggcagc acgcatgagt tcacagggtg
     2281 ccttgccgta tgaggtgaga caaggcatga gaaaaaaatc gtgacgatgc cgtgggtaga
     2341 tgaaagacaa acggagtgaa tttccgtcgt ttggtcgctg ccaggcggaa gcaggggttt
     2401 acatgatgtt ggttccggtc ctaatagaac gatcaccact cgatcggaat gcgactgctc
     2461 attcgaatct ccttgttgtt cctgttggcg gggcttcatg cctcgtgtcg tcccgctctg
     2521 cccagcgttg gtcgaggtgc agccacctcc gagtcgccgc aggaaccatg ggacctgtcg
     2581 tcggcgccca gcgtcgaagg ggcaacgtcg cgagggtcgg aggttgttcc gacgttttcg
     2641 ccgaaacaaa gcgcacccgg aggaaagctc acggtaggca tggccgtgga ccttgcgatg
     2701 cgtaacgatc cgcgcacgca agcgacatgg tttcgagcgc gagcggcagc cgcgcgcctg
     2761 gggacatcgg aagcgggtta ttatcctcgg ttggatgtgg gggccacggg ttcgtataat
     2821 catcaaaccc tgcaaggtgg ccggatgtct ttgacacaag gcacgatcgg tccgtatgcg
     2881 aagttgagtt ggctgctgtt ggatttcggg gcgcggtccg ccaatgagca agcggaactg
     2941 gcggccgtga ttgcagccaa tggtgcccat ggcgcagctt tacaagacca agtgttgttc
     3001 gtggtggatg cgtattatcg ctgcgtcagt gcggaagcgt tggtcgaggc ggcgaaagcg
     3061 gatgaaaaga ccgccacttc gagcctggag gcggcgacag ccttgcacgg ttcgggaaag
     3121 gcggcgttgg gcgatgtgct tcaatcgcga acctcgctgt ccagagcgcg catggcggtg
     3181 tcgacagcac aggggcaagc acgcgtgatg cgcagcgccc tcgcggtaca gctagggatg
     3241 actcccgatg tgcaattcga gttggagccc ctggcagacg atgtacatgc gcaagacgtc
     3301 acggagggcg cggagcagtt gctcggcaag gcgcttcggt cgcgtccgga tctggtggcc
     3361 atcaaagcgg cagcggctgc ttcctggcaa cgcgcggaat ctgtcaagca tcggggcatg
     3421 cccacgcttt ctctcgatgc gaatgcgagt cgtggctact acctgccgca aacctacggg
     3481 acccatgggg ataattgggg cgtgtcgcta accttcaata taccaatttt tgctgggttt
     3541 tccgatagtt acgaacagca gcgcgcccag tgggaagcca tggcggccga ggccgacgcc
     3601 cgcgcgctcg aacgacgaat tgctctggac gtgtggaccg catggttgcg tcttcgcacg
     3661 gccgcggagc aagtggatga ggcaagcgct cttttgaaga tctccatgga atgggaacag
     3721 cttgccaggg ggcggtacga agcaggggtg gggacgattc tcgacctgat ggctgctcaa
     3781 tcttccctgg catcagcacg ttctcaacac gtcaaggcga gagcggagtg gattcttgct
     3841 cgggttcaat tggcccacga tgcaggcgag ttggggcgaa gaacgcttac tgatgttcag
     3901 caaagcgtgc tcgagggtgg ctcggttccc tccacgccca cggcagctcc acgcgggtct
     3961 tcccttgctc ccccgatatc gaggtaaccg tggaccatct tcctcgtcgc ttttcgcccg
     4021 gtttttcctt ctggcccatc gtgtttctgt ggctcgtgct gcttttgccc gcctgtcgcg
     4081 acagccaagg gcagcaacaa ggacgctcct ctcgtccggt acccgtcctt gttgaaaaag
     4141 ttgtacgaaa aacaatgccg gtcgacatca aggccaccgg aagtatcgaa gcttattccg
     4201 tggtgtccgt gcaggcacaa gtgggcgggc agcttcaaaa agttggattc aacgaagggg
     4261 atatggtgcg caaagaccag gtcctcttcg tgttggatcg tcgccccttc gatgcagcca
     4321 tgcgcgagtt ggtggcccga agggatcgaa atcaggttct gatcgacaac gcgaaacggg
     4381 agctggctcg ttacgactcg ttgctcgaaa aagactacgt ttccaaagag gattacgatc
     4441 gcgccaaagc caatgtcgat gcgttggaag cgtcgctacg cgccgatcga agcgccgtcg
     4501 atgccgcttc gatcaacttg caatacgcca ccattcgctc tcccatcgaa ggacgaaccg
     4561 gaagcttgct cgttcaaccg ggcaacgtcg tgaagcccaa cgataaagcg ctcgtggtca
     4621 ttcgtcaggt caaacccatc ttcgctcgct tttccgtgcc cgagcaatat gtggcaagca
     4681 ttcgacagcg aatgcgggaa ggaaaaccgt cggtcacggc tcacgtgcgt gatggttccc
     4741 cggaacctga acatggtgaa ttgacgatgc tcgaaaacac cgtggacagt gccacgggca
     4801 cgatcgaact caaagcgttg ttcgccaacg agctggaacg gctctggccg gggcaatatg
     4861 tggatgtggt gctccagctt cgcgtgcaac aagacgcgat tgtcgttcca gcgtccgcga
     4921 tacaacagag tcgtgaaggt gaattcgttt atgtggtgag cgcggaggga gtggcttcgg
     4981 tacgtaaagt cgttgtgaat cgttccgtgg ggcaagaaag cgtgatcgag agcgggttgt
     5041 cacccgacga ggtggtggtg accgatggac aattgcagct cacgtcgggc tcgaaggtgg
     5101 aaatcaagac gggctcaccg acgcgcgcag tcgttccgtc cggctcggct tctgctgctc
     5161 ccgtagggag ttcatcacca tgaacgtatc cgcgccgttc gtcaggcgcc cggtgatgac
     5221 caccctggtg acgttcaccc tgctgctcat agggttgatt gcgtatccgc tgcttcccgt
     5281 cagcccacta ccgaacgtgg actttcctac catcgtggtg atggcctcgt tgcctggggc
     5341 gagtcccgaa acgatggcgg catccgtggc cacgccgctc gaaaaagagt tttcgaccat
     5401 cgatggcgtg gactcgatga cgtcgaccag ctccttgggc aacacacaga tcacgttgca
     5461 attcgatctt cgacgggaac ttgatggtgc agctcaggat gtgcaggcgg cgatgtcgcg
     5521 ggcggcgagg cagttgcccc gcgacatgcc gagtccacct tcgtatcgaa aggtcaatcc
     5581 cgcggaagag ccaatcattc gtatctcgct gagttcggcg acactgccgc tgcacgtggt
     5641 caacgagtat gcggagacga tgcttgcgca gcggttgtcg atgttgcgcg gcgtggcgca
     5701 agttcaggtc tatggctcgc agaagtacgc ggtgcgcgtt caggtggacc ctcgcaaact
     5761 caaggctcgg ggtatcggaa tcgacgaagt cgcgcgagcc atccaatcct cgaacgtcaa
     5821 tatgcctacg gggacactct atggtccttc gaaggcgtac acgatcgatg tcgacggtca
     5881 gcttacgcgc gcgaaagatt atggtccgat catcgtctct tacgccaacg gaagtcccgt
     5941 gcgtgtggag caactcggca aagtgctcga tggcgtggaa aacgacaagg ttgcggcatg
     6001 gctcacgaac gatcgcgcca ttgtgcttgc ggttcaaaag cagcctggcg ccaataccgt
     6061 ggaagtgtcc aagtcgatcc gcgaagtgct tcccagtttc gaggcggatt tgcccgctgc
     6121 cgtcaacctg aagctgcttt acgatcgaag cgaatcggtg cgcgaatctg tcaatgacgt
     6181 tcagctcacg ctgctcatca cccttgggct cgtcatcggc gtcatctttg tttttctccg
     6241 caacgtcagg gccacgatca ttcccgcggt ggccatgccg ctgtcgattt tgggcacgtt
     6301 cgccgcgatg taccttcttg gttatagcct caacaacctg tcgctcatgg ctttgacgtt
     6361 gtcggtgggg ttcgtggtcg atgacgccat cgtgatgctc gaaaacgtcg tgcggcacat
     6421 ggagatgggc aagaaaaggt ttgaagcggc cctggaaggc tccaaggaaa tcgcgttcac
     6481 gatcgtgtcg atgacgctgt ctttggcggc ggtattcatt ccgatcttgt tgatgggagg
     6541 catcgttggc cgattgttcc gtgagttcgc ggtaaccatc acggtcgcga ttctcgtgtc
     6601 cgggtttgtg tcgttgacgc taagcccgat gatggcgagt cggattcttg gggaaaatgg
     6661 cgggaagaag ggaaaggcct atgctttcac cgaacgggtc ttcgatgcca tcgtggcctg
     6721 gtatggccgc actcttcacg tggtgctgcg ccatcgaagg ctgacgatgg tgttttcgtt
     6781 gctcgtgctg ggagcgacgg gatacttatt tcaacgtgtt cccaagggat ttttgccgac
     6841 ggaagacacg ggacagcttt ttgcgctcac ggaagccgcg gaaggaatct ctttcgaggc
     6901 gatggtggag cttcagagaa aagtcgccaa gatcgtttcc gaaagcccgc atgtggaagc
     6961 tttcatgtcg agcgcgggca atcgtggggg gagcttgggc agcaacaccg ggatgctctt
     7021 catcaatctg aagccgcgtc gagaa
//
LOCUS       DAOSOR010000294         7036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10742_length_7036_cov_3.645037, whole genome shotgun sequence.
ACCESSION   DAOSOR010000294 DAOSOR010000000
VERSION     DAOSOR010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10742_length_7036_cov_3.645037"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PK710_15160"
     CDS             <1..486
                     /locus_tag="PK710_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11117.1"
                     /translation="LSLALIRAGAYEQASHQMVVSRNLPSTTPYERGEILDRLDATMA
                     AVRQKRVQAAKFDEPDSSILRAEAEFEVGGWLRAARILRLAHQRFPDRPEVWQRYVRA
                     LAQCRLDDDAYTVALLHLDPAAAQELLASIRKEMSPRTAQAVPVPLGTRWWEESPLIE
                     P"
     gene            660..3416
                     /locus_tag="PK710_15165"
     CDS             660..3416
                     /locus_tag="PK710_15165"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT11118.1"
                     /translation="MSRPQAFGAYDLLERINVGGMAEVFRAVHRQSRRLVAIKRILPS
                     VAEDDEFISMFHDEAIIASQLDHPNIAKIFDIGKVDASYYIAFEYVNGRDMRTVIDNA
                     EHRQASIPIDMTLHVIMSLCKGLDYAHRRVDANGQPLGLVHRDVSPPNILVSYDGDVK
                     LIDFGIAKAAGKLARTQVGSIKGKFGYMSPEQVRGLPVDQRSDIFAIGICLWEFLTCR
                     RLFLGDNEVVVMGRIRNAEAEPPSQFAPSLSPELDQMVMRALAKDVDERYTSASDLFA
                     DLNAFAQKAGITVSRDPVADYMRRTFPNDASLRVASHEEAHAMAENKGGSDLDVFEGL
                     ARKPQARPATTPSTVPSPLGVASSSGPPPPGRARTLLGMAPPMPPSAHGSMPSSPGRL
                     PIPSSPGLPPPPVARGSGVLPAISAPPAKGSAPPPARGTLPSNPLPPPVAPPVMGSGV
                     IGPPPAKPAAVDMDWDDEDEKTHVYDKQSTDEVADAIMQPLPAAGTPAPPPTSSAAAA
                     LLQRSGNVAPPMPKVPSIPPPTPGLVPAPPPRTVAAAEPTMLSAPKEKASSLPWILVA
                     ILVLVAGGLGAYFLMPSTGSLKVYVSGPGGRDIGKVNIFVNNEKVCSSSPCTVNDLKE
                     GIHDVKAEAEGYEPTAPKGVEVKGRAQAKLDLELAVASAGSGFKVAGAQQGIMLFVDG
                     SEVGPLPQTLKTLSPGEHKLRFVGSERFEPLEKTLTVVPDKIEDLGEIKLNVLKGKAT
                     LQLVTRGARILLVPSKGEKRQIDERMFKNDQLSIDIDTSKEWSIEATKPGYDDLKIPI
                     SFADGQAEKTFRIELHERGKTPVDDDRKQSSSTAPTSGPGPTPASTPTAATTGGPATL
                     NINSIPPSAVLLDGRPIGRTPKSGVVVSPGTHSITFIHPEKGRKSTSVTVKAGETKGV
                     GVRF"
     gene            complement(3489..4721)
                     /locus_tag="PK710_15170"
     CDS             complement(3489..4721)
                     /locus_tag="PK710_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11119.1"
                     /translation="MQLLPLARPFLIPAVVFCFLLAVFFDLAPRLSCPEEPWSLLPLP
                     PFPSYLLHFIATIAVFTVLLALSNAPSAMLASLSFGLLPVTVDVAVVSPYELAAVATA
                     LMAVLLHLRPHLVTRVVCMVFYALAAAWSPSVWIVPILLISQDILYQKRPFRRWALWH
                     LAMVATALAHLFAHAPMIKSVNGNPIRAMATHLVLSFDSLLVPWTTRLSDTMSLPFTI
                     IVVLGAASACALAVVLAYRSPCEASRLMAWGSVWFIASVLSTAMLGSSRRAILFPLIG
                     LAFVLAAAFFWLWTWTRQWLARGSYRAVFGVGIGALWLGLAALSARRAHEWSSDETAL
                     RLHCEAEMRFGYWEGAEEACNKALQRDENQVTTRYSLALSLRNLGRDKEAAWQVHQIL
                     EEHPDHSGARKLLATIKE"
     gene            complement(4725..5651)
                     /locus_tag="PK710_15175"
     CDS             complement(4725..5651)
                     /locus_tag="PK710_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAT repeat-containing protein"
                     /protein_id="HOT11120.1"
                     /translation="MTSRSLLIGLVLLACAVMIGLSRPRLVEWLSQAKETSDVYVLPP
                     PTVLARASLGYRAAVADYLWAHVLVTQGLRMGAKRPFQEVGQYLDAINHLDPAYREPY
                     RLADSLLSHQAGDSDRAQSVRRARQILERGLIQFPYDAELWLNYGQFLAYIGPGALQF
                     DSEEWHAWRNEGAKAIVRAGELGASDEAMVFRSMSATTILNKAGEVDAAIRFLERLYT
                     ATDNEEARDDILRSLESLRAGKAASRDFELAKKFDSLWRADLPFASRVMLSVLGPPVD
                     AWKCAGPNPEAFCDRTWIGWSNHAMSATGKNP"
     gene            complement(5648..6136)
                     /locus_tag="PK710_15180"
     CDS             complement(5648..6136)
                     /locus_tag="PK710_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11121.1"
                     /translation="MRAVSPVELAVVVSIAGSVLAASVPTFVRHVHASRMTEAVDGLS
                     KIGAGAIAHAQGKELAASFPPPAELTPKDVPRGVAAEDPPGTWDSPTWQALGFRFDVP
                     HRYAFQFDVVPDPSRIWFQATAKGDLNGDGILSTFALAGERRVGEQAVLIPGIYIERE
                     VE"
     gene            6240..6893
                     /gene="lepB"
                     /locus_tag="PK710_15185"
     CDS             6240..6893
                     /gene="lepB"
                     /locus_tag="PK710_15185"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOT11122.1"
                     /translation="MDLRKVGKFVLWTSIIAGVTWGALRLLLVKTWTVPSDDPALTSS
                     IAPSLGPGDLVLLLHGLPIGFGDLVRCKDPDEPRRWVIGRIAGEAGDTVELNSSRLLI
                     NGRMADVDHACAPPKIKVPHPNTEGEVELHCDMEDLGGVIHMRAHGNPEGRAQSAPVK
                     RTVPPNSYFLISDNRAYPSDSLTYGAVPTDSCDARIIFRIWSSKGFGDAERRFTWVN"
BASE COUNT         1627 a         2040 c         1964 g         1405 t
ORIGIN      
        1 ctttctctcg cattgattcg ggcaggagct tatgagcagg cgtcgcatca gatggttgta
       61 agccgaaacc tgccgtcgac cacaccgtac gagcgcgggg aaattctcga cagattggac
      121 gcaaccatgg cggccgttcg acaaaagcgc gttcaagcgg ccaagttcga cgaaccggat
      181 tcatcgatat tgcgcgcgga agcggagttt gaggtgggag gctggctgcg tgcggcgcga
      241 atcctgcgat tggcccatca acgcttccca gaccgtccgg aagtatggca gcgttatgtg
      301 cgggcgctcg cgcaatgccg tctggacgac gatgcttata ccgttgcctt gctgcacctg
      361 gatcccgcgg cagctcagga gctattggct tcgattcgga aagagatgtc cccgcgcacg
      421 gcgcaagcgg tgccggtgcc tttgggcacg cgatggtggg aggaaagccc acttatcgaa
      481 ccttgattcg cgccttgggc aaagatacgt cacggaccga aaacacttat attggtattt
      541 ttttttgtga ctgcatcgac ggattcgatg tcgcccaaca ttgaccttcc ttgctatcgc
      601 gcggtacaag ctccatcggc ctatcttatc gtaagacgtg tgtgtttgga gcattgcagg
      661 tgagccgtcc tcaagccttc ggagcctacg acctactgga acgcatcaac gttggtggta
      721 tggccgaagt atttcgcgcg gttcatcggc aatcccggcg gcttgtggcg atcaagcgga
      781 tcttgccatc ggtggcggag gacgacgagt tcatctcgat gttccacgac gaggccatca
      841 ttgcgagcca gctcgatcat ccgaacatcg cgaaaatctt tgatatcggg aaggtcgatg
      901 ccagttacta catcgcgttc gagtatgtga acgggcgtga tatgcggacg gtcattgaca
      961 atgcagagca ccgtcaggca tcgattccca tcgacatgac actgcatgtg atcatgagtc
     1021 tttgtaaggg gttggattat gcgcatcgac gggtggatgc gaatggtcag cctctcgggc
     1081 ttgttcaccg cgatgtgagt cctccgaaca tccttgtttc ttatgatggg gatgtgaaat
     1141 tgattgactt tggaatcgcg aaagcggcag gcaagctggc gcgtacgcaa gtaggctcaa
     1201 tcaaagggaa atttggctat atgtctccgg agcaggttcg cggtcttccc gtggatcaac
     1261 gttcggatat ttttgcaatc gggatttgcc tttgggaatt tttgacttgt cgtcgattgt
     1321 ttcttggaga caacgaagtg gtcgtgatgg ggcggattcg taacgcggaa gccgaaccac
     1381 catcacagtt cgcgccctcg ttatcgccag aacttgatca gatggtgatg cgagcgctag
     1441 cgaaggacgt ggacgaacgc tacacttcag catcggattt atttgcggac ttaaacgcat
     1501 tcgcacaaaa ggctggaatc acggtttccc gtgatccggt ggcagactac atgcgacgga
     1561 cctttcccaa cgacgcgagc cttcgcgtcg cgtcgcacga ggaggctcac gcaatggcgg
     1621 aaaataaggg cggttcggac ctcgacgtat tcgaggggct cgcgcgcaag cctcaggcac
     1681 gcccagcgac aacaccttct acagtaccga gtccactggg agtggcgagt tcttcggggc
     1741 caccacctcc ggggcgagcg cggacacttc tgggcatggc accacccatg cctccctctg
     1801 cgcacggctc gatgccttca tcgccggggc ggctccccat tccgagttcc cctggtttgc
     1861 ctcccccgcc ggtggcgcgc ggttccgggg ttcttccggc catttcagcc cctcccgcga
     1921 aaggaagtgc gcctcctccc gcacggggaa cgcttccctc caatccgctt cctccccctg
     1981 tagctcctcc cgtcatggga agtggtgtga tagggccacc gcccgccaaa cccgccgcgg
     2041 tcgatatgga ttgggacgat gaggacgaaa aaactcacgt ctacgacaag caatccaccg
     2101 atgaagtggc tgatgccatc atgcagccac ttcctgccgc gggcacgccc gctccgcctc
     2161 cgacttcttc ggctgctgcc gcgcttctcc aacgctcggg caacgtggcg cctcccatgc
     2221 ccaaggtgcc atcgattcct ccgcccacac ccgggctcgt tcctgcgcct ccgcctcgaa
     2281 cggtagcagc cgctgaaccc acaatgctct ccgcgcccaa ggaaaaggct agttcgctgc
     2341 cttggatctt ggtcgctatt ctcgttttgg tcgccggtgg acttggcgcc tacttcctta
     2401 tgccttccac cggatcgctc aaagtctatg tttcaggacc aggcggacga gatatcggca
     2461 aggtcaatat cttcgtcaat aacgaaaagg tgtgcagctc ctcaccatgc accgtcaatg
     2521 atttgaaaga gggcatccac gacgtcaaag ccgaagcgga aggatatgag cctacggctc
     2581 ccaaaggtgt cgaagtcaag ggaagggcgc aagcaaaact cgacctcgag cttgcggtag
     2641 cctccgcggg cagtggattc aaagtcgctg gagcccagca aggcatcatg ctttttgtcg
     2701 acggctcgga agtaggaccc cttccccaga ctctgaaaac cctcagccct ggtgagcaca
     2761 aactccgctt cgtcggctcg gaacgctttg aaccccttga aaagacactc acggtggttc
     2821 cggacaagat cgaggacctt ggcgaaatca agctcaatgt gctcaaagga aaggctacat
     2881 tgcagctcgt cacccgcggc gctcgtatct tgctcgtacc ttccaaaggt gaaaaacggc
     2941 agatcgatga gagaatgttc aaaaacgatc agctctcgat cgatatcgat acgtccaaag
     3001 agtggagcat cgaagccacc aaacctggtt acgacgatct caaaattccc atctcatttg
     3061 ccgacggcca agctgaaaaa acgtttcgca tcgagttgca tgagcgtgga aaaacacctg
     3121 tcgatgacga tagaaagcaa tcctcttcca cagcccccac ctcgggccct ggtcccacgc
     3181 cagccagcac gccaacggct gccactaccg gcggacctgc cacgctcaac atcaactcca
     3241 ttccgccttc ggccgtgttg ttggatggtc gtcctatcgg cagaactcct aaatccggcg
     3301 ttgtcgtgag ccccggcaca cactccatca cgttcattca tcccgaaaaa ggacgaaagt
     3361 ccaccagcgt gaccgtgaaa gcaggcgaaa cgaagggcgt gggcgttcgt ttctgatcca
     3421 agcttttcca ccccaaacaa tccaacccca actcgacggc gaataccgat cgattctccc
     3481 tgaccttgtc attccttgat cgtcgcgagc agcttccgtg ctcctgaatg atcaggatgc
     3541 tcttcaagga tctgatggac ttgccaagca gcttccttgt ctcttccaag attccgaagc
     3601 gataaagcca atgaatatcg cgtagttact tgattctcat cgcgctgaag agctttgttg
     3661 cacgcttctt ccgccccctc ccaataacca aagcgcattt ccgcctcgca atgcagtcgg
     3721 agagccgtct cgtcgctgct ccattcatgc gcgcgacgcg ccgaaagcgc tgcaagcccc
     3781 aaccacaatg ctcctatgcc tacgccaaac accgcgcgat acgatccacg cgccaaccat
     3841 tgccgtgtcc atgtccacag ccaaaagaac gccgcggcca acacgaaagc gagtccgatc
     3901 agaggaaata agatcgctcg tcgactcgaa ccgagcatcg ctgtcgacaa aacggaagcg
     3961 ataaaccaaa ccgatcccca agccatcaat cggctcgcct cgcatggact cctgtaggcg
     4021 agtaccacgg cgagggcgca ggccgatgcg gcgcctaaca ccacgatgat ggtgaacggc
     4081 aagctcatcg tatccgatag ccgcgtggtc caaggcacca acaacgagtc gaagctcagg
     4141 accaagtgcg tagccatcgc gcgtattggg tttccattca cggacttgat catcggagcg
     4201 tgcgcaaaaa gatgagccaa ggcggtggcc accatagcca gatgccaaag cgcccatcgt
     4261 cggaacggtc gtttctgata aagaatatct tgggaaatca ataggatagg tacgatccag
     4321 acgcttgggc tccacgccgc ggccaaggca tagaacacca tgcagacaac tcgcgtgaca
     4381 agatgagggc gcaagtgaag gagcaccgcc atgagagcgg ttgcgaccgc ggcaagttca
     4441 tagggggaga caacggcaac atcgacggtc acgggtaaaa gcccaaagga cagcgatgcc
     4501 agcatggccg acggcgcatt ggagagtgct aacagcacgg tgaaaacggc gatggtagcg
     4561 atgaagtgca gtaggtagga tgggaaagga ggtaggggaa gcagggacca cggttcttcg
     4621 gggcaagaaa gccgtggggc caggtcgaag aaaacggcaa ggagaaagca aaaaaccacg
     4681 gcaggaatga gaaaagggcg cgccagggga agcaactgca tgggtcaggg attcttgcca
     4741 gtggccgaca ttgcgtgatt ggaccaccca atccaggtcc gatcgcaaaa cgcctccggg
     4801 ttgggcccag cacacttcca agcatcgacc ggcgggccga ggacactgag catgactcgc
     4861 gacgcaaaag gaagatctgc tcgccaaagg gaatcgaatt ttttggccaa ctcgaagtca
     4921 cgggaagcag cttttccagc acgaaggctt tcgagacttc ggaggatgtc atcgcgagcc
     4981 tcctcattgt ctgtcgccgt atagagccgc tcgagaaatc gaatggcagc atcgacttcg
     5041 cccgccttgt tgagaatcgt ggttgccgac atcgaccgaa acaccatcgc ctcatccgaa
     5101 gcccccaatt cccccgcgcg aacgatcgcc ttggcccctt cgtttcgcca ggcatgccat
     5161 tcttccgaat cgaattgcaa cgccccagga ccgatatacg caaggaattg accgtaattg
     5221 agccataatt cggcgtcgta ggggaattgg atcaagcctc gctccaatat ttgccgagcg
     5281 cgtcgaacgg attgcgcccg gtccgaatct ccagcctgat gggaaaggag cgaatcagcc
     5341 aggcgataag gctctcgata agccggatcg agatggttga tcgcatcgag atactgacca
     5401 acttcttgaa acggacgctt ggcacccatc cgtaatcctt gcgtcacgag aacatgagcc
     5461 cataaataat cggccaccgc agcgcgatac cccaacgagg ctcgtgcaag aacggtgggc
     5521 ggtggaagca cgtacacgtc cgaagtttct ttggcctggc tcagccactc cacgagacga
     5581 ggccgcgaaa gaccgatcat cacggcgcaa gcgagcaaca ccaaaccgat caaaaggctt
     5641 cgagaggtca ttccacctcc cgttcgatat aaatccctgg aatgagaacg gcttgttccc
     5701 ccacccgacg ctcaccagcg agggcaaagg tagacaggat tccgtcaccg ttcagatcgc
     5761 ctttcgcggt ggcttgaaac cagattcggc tgggatcggg tacgacatcg aattgaaaag
     5821 cgtagcgatg cggcacatca aatcgaaaac caagggcttg ccatgtgggc gaatcccatg
     5881 tgccgggtgg gtcttccgcc gctactcccc gcggcacatc cttgggagta agctccgcgg
     5941 gagggggaaa ggaagcagcc aactcttttc cctgcgcatg agcgatagct ccagcgccga
     6001 tcttcgacag cccgtccaca gcctccgtca tccgcgaagc atgaacgtgc cgaacaaacg
     6061 tgggcaccga agccgccaac accgatccgg cgatcgacac gacgacggcg agttcgactg
     6121 ggctcaccgc acgcatagca gggtgataga ttgagacttg gcctttcgtc aagggaggca
     6181 aacgcctcgt tcccatcgaa aaccttgcgc tgcctcacgt tccgaacgac aatccgggtg
     6241 tggaccttcg aaaagtcggc aaatttgtgt tgtggacatc gattatcgcg ggtgtgacct
     6301 ggggcgcgtt gcgtctgctg ctggtcaaaa catggaccgt tccctccgat gatcccgcgt
     6361 tgacctcttc catcgcgcca tccctcggtc ctggagatct ggtgctgttg cttcatgggc
     6421 tgcccatcgg ctttggtgat ctcgttcggt gcaaggatcc tgacgaaccc cgtcgctggg
     6481 ttattggaag aattgcgggc gaagccgggg atacagtgga actgaattcc tcccgtttgc
     6541 ttatcaacgg acggatggcg gacgtggacc atgcctgtgc gccgcctaaa atcaaggttc
     6601 cgcaccccaa taccgaagga gaggtggaac tacattgtga tatggaggac ctgggagggg
     6661 tcattcatat gcgcgctcat ggaaatcccg aaggtagagc ccaatccgca ccggtcaagc
     6721 gaacggtgcc tccgaattcc tattttctca tcagcgataa tcgcgcctat ccgagcgact
     6781 ccctgacgta cggagcggtc cccaccgata gttgtgacgc gcgaatcatt tttcggattt
     6841 ggagtagtaa aggatttggg gatgcggagc gacgatttac gtgggtgaat tgaccggaag
     6901 agccgtcaac cttattcctc aggcatgttt tcggcgaagt tcggggcaat ggtaaggacc
     6961 aagccgccag aggtggcaga ctggatgcta ccgccaagag aaaacatgga tgtgacagtg
     7021 tcgccattca agtcac
//
LOCUS       DAOSOR010000295         7022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10772_length_7022_cov_3.993254, whole genome shotgun sequence.
ACCESSION   DAOSOR010000295 DAOSOR010000000
VERSION     DAOSOR010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10772_length_7022_cov_3.993254"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1260)
                     /locus_tag="PK710_15190"
     CDS             complement(52..1260)
                     /locus_tag="PK710_15190"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT11123.1"
                     /translation="MNERRARIRSPNQLAPGTEVQGYRIVRKLAVGGTAWVFEGQHIE
                     SNRRVAIKVLSDQYRGDSCLVRRFANEARAVMTLDSPHIITIESVGTLSAGLPFIVME
                     YLDGLDLYHLLEQYGWRMPVDVALSIVDQVAQALELAHAHGIIHRDIKPENIFVLATS
                     DGLLAKVLDFGLSRVPRAGENSSLTRTGTTVGTPHYMALEQLRGDRTIDGRADVYSLG
                     VLTYELLSGHCPFEGPDLHEIMQRAAREQAPSLHDYRPDLPEGLVQAIAHAMTRDRNK
                     RCPSARAFREAIRPFWNNESTQPESSPTRGSSVVVQPERNDSEAPVPTASTTSHAPST
                     TVSPPKASPPPTPHPRSTQRPAPRHNPLATIAAVVFAALMLSLGLTCFVVDTYQQTQG
                     TAKASLPSAP"
     gene            1615..2892
                     /locus_tag="PK710_15195"
     CDS             1615..2892
                     /locus_tag="PK710_15195"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOT11124.1"
                     /translation="MTWLLAVGVALVVFAFVVHRLASKQTAPAIRGAEPKRHSLEDSN
                     RTHRSSKDKSKGKADSVPSSSPNGKKAKPSKGAKSSPALKVSPAVLESIRPLSVPPPA
                     NDFDDEWEDVDVTVVAELPDEIKAMQKGYRSVDPKQAVAQIAEEYDDTAEIEIIEERL
                     VDGLLVEELLNDEDTGPNALMLPIGEVLTDVGRRRPNNEDRPLSLPEHFVFGVADGMG
                     GHAAGEVASKIAMETIEKAFEDNVFEGEPNSLWPRRGDELARGIEMANRNIFRVAMED
                     DALSGMGTTVTAIRFCPERQRAYIAHVGDSRCYRFREGQMRQLTKDHTLGAELGAKGK
                     MATHLSRSVGIAETVEVDLMVDAPRVGDRYLLCTDGLTKMLSEERILEISTDGIVADC
                     VRHLIDEANDNGGKDNITVALVEIREPVGTGMT"
     gene            2923..4029
                     /locus_tag="PK710_15200"
     CDS             2923..4029
                     /locus_tag="PK710_15200"
                     /inference="COORDINATES: protein motif:HMM:NF023327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3419 family protein"
                     /protein_id="HOT11125.1"
                     /translation="MSVSDLRYFRHLNYSLGNEDSAVEWAVLPEGVGRVLCVAGCGSR
                     VLPLLARKPARVVCADISASQLWMTELRVEAARALNHAEYLQFFGYPPNPGSPEQRRR
                     LFHRLALSDEARAYWLDVFDHRDWQTVLYDGRWERTFRKLSRINAMLTGGRGRALFEA
                     RTREQHREYMRERFPRRAWLMVLLLLGNPLVFNLMLYKGDFPRKNIEETPFSFYCRAF
                     DRLFSLGPARENFFLQIVFWGELRFAEGNPIECDPGVFAKIQEALGSATIDYRLGDIL
                     EIAHREGGHFDFVSLSDVPSYFVPPREQTYLQDLRPALSSRARVVLRYYLRRPERAEK
                     RGYGNLTERYASAIAAEKVGVYDIEVLEKLEDNR"
     gene            complement(4082..6169)
                     /locus_tag="PK710_15205"
     CDS             complement(4082..6169)
                     /locus_tag="PK710_15205"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT11126.1"
                     /translation="MLSISPSKDLMPSPTSQPSPWPAFLTIGLLLTTINIAAILSAAL
                     AWCIAGQFRWIVALTGAFGNITPGLLFTTLLFVWLHKRKALFFISLGLLFVVQTITTI
                     TCTEFMLQRGAPIAPIDVLNGLDAGFLNSQLGVLTSSHVGPYLALSIAVFALSLSLLH
                     RQWIPIPRQGILIVLAVLGTFILLSLGIHRTDRGKHALRPYDRLLGALVTTNHLNIIV
                     GLEPALKKQNVTDSSALPALAMIGLPIQHCPDGLHVAEPITQSSKNTLVYNRLYELGI
                     HLGGRGRPLSLLFIMLESVGAEDIACLDAGAPHGLTPYLDDLIQRTPHVLVGKRFHQG
                     GQRTAGAMSSLLCGVGTGPFGIAPLRDLPGLKLRCWPDLVAEAGGDLRFFYADNLRFD
                     RYDETLLEHGFRYLHTPRTQGRARGAWGLSDQELFADVLADLAQTSAGITPQATRIRG
                     VLTLSTHGPFDIPQDMPADLRDRSRDLVHKSTQDSVKRAHWTTVAYLDHALSRFIPAF
                     MQAEEKSGRTAVVMMVGDHTSGVGTTTNPLQRSRIAPIWVFPSDTDVARVTSAQRELD
                     AHGWSQNDLPRMLVAFLDGFGALRSLAPSQRWHTLGGQAMSPTFAIIPPWSDTHLWTI
                     DTLARSRMLDASDEIIVEEIAESPGSFTDLETSTKAMDKALPALSWMLQHPDRLHPCA
                     NAPAPASSAVTPH"
     gene            6277..6969
                     /locus_tag="PK710_15210"
     CDS             6277..6969
                     /locus_tag="PK710_15210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT11127.1"
                     /translation="MGMQDNTLLYDVCDLSFVYELGRQKVHALRNVSLQVKRGDFVCL
                     AGPSGSGKTTLLSVLGLIEPLQQGTVRFEGRSLSEMTETDRNRLRRFHIGFVFQQFHL
                     IPVLSAEENVEYFLTRQGVPKAERRKRVDEALETVGISSQRKQRPLEMSGGQRQRVAL
                     ARALAKRPNVIIADEPTASLDQATGKEIMRIFAEQTRERGVSVIAASHDPMVFAFAAR
                     QIELRDGEVVSC"
BASE COUNT         1479 a         1595 c         2407 g         1541 t
ORIGIN      
        1 gtggctggct ttgggaaagg ggaagaaggc ggattcggat ttatgaagcg gtcatggagc
       61 cgaagggagg gaggctttgg cggtcccctg ggtttgttgg taggtatcga cgacaaagca
      121 ggtcaaaccg agggagagca tgagcgcagc gaacacgacg gcagcgatgg tagccagagg
      181 gttatgccgg ggcgcgggac gttgcgtcga gcgaggatga ggggtgggag ggggggaagc
      241 ttttggaggt gagacggtgg tcgatggcgc atgggaggtg gtggatgccg tagggacggg
      301 ggcttcggaa tcatttcttt cgggttggac aacgaccgaa gagccgcggg tgggtgaaga
      361 ttcgggttgt gtcgactcgt tgttccaaaa gggtcggatg gcttcgcgga aagcgcgggc
      421 cgacggacag cgtttgtttc tgtctctcgt catcgcgtgg gcaatggctt gaacaagccc
      481 ctccggcagg tcgggacgat agtcgtgcaa ggatggagct tgttctcttg cggcccgttg
      541 catgatttcg tgcagatcgg gtccctcgaa ggggcaatgg ccggacaaca attcgtaggt
      601 gagcacgccg agggaataga catccgcgcg gccgtcgatc gtgcggtcgc cccgtagttg
      661 ttctagcgcc atataatgcg gagttccgac ggtcgtgccg gttcgtgtga gcgacgagtt
      721 ctcgccggca cgggggaccc gcgacaatcc aaagtccagg actttggcga gaagcccgtc
      781 cgatgtagct agaacgaaga tattttcggg cttgatatca cggtggatga ttccgtgcgc
      841 gtgggccagt tcaagggctt gggcgacctg gtcgacgatg gataaggcga cgtcgacagg
      901 catgcgccag ccatactgtt cgagcaggtg gtacaggtcc aagccatcga ggtattccat
      961 gacaatgaaa ggaaggccgg cggaaagggt gccgacgctc tcgatggtga tgatgtgcgg
     1021 cgagtccagg gtcatgacgg cccgtgcttc gttggcgaat cgtcggacca ggcaagagtc
     1081 gcctcggtat tgatcgctga ggactttgat ggcgacgcgt cggttggatt cgatgtgttg
     1141 tccttcgaag acccaggcgg tgccgccgac ggcaagtttt cggacgatgc gatagccctg
     1201 cacttccgtt cccggcgcta gctggttggg agaccggatt cgggcacgcc gctcgttcac
     1261 atcgacctcc tgagaagggg cactgcaaaa gggatatgca aagatcaaac ccggtgaact
     1321 cagaaattaa tataataata ttcgttagtt acagtaaggg tgagcgaatt gatcaggaac
     1381 ggatcgatcc ccttgagaag gaattcttct cacttgatcg acgggaaggg ggcgtttgca
     1441 tagagcagtt ttccgtgtct ttcaccattg gacaaaacga gattcacggt tcaggggctt
     1501 gactgtgggg gaggcgacga tccggtctgg gcaggagagg tgttcttgtt ttaggatgac
     1561 ctgacaactc gtgaatcgcg ttcgaacatt gtatggcctc gagggtgcca tctcatgact
     1621 tggcttctcg ccgtgggcgt agctttggtg gttttcgcct tcgtggttca tagattggcg
     1681 agcaaacaaa ctgcgccagc gattcgaggg gcggaaccca aacgccattc gctcgaggat
     1741 tccaatcgga cccatcgttc gtcgaaagac aaatcaaaag gcaaagccga ctccgtcccg
     1801 tcgtcgtcgc cgaatggcaa gaaggcaaag ccatccaaag gggctaagtc atctccagcc
     1861 ttgaaagtat caccggccgt gctcgagtcg attcgtccct tgagcgtgcc tcctcccgcg
     1921 aatgactttg atgacgaatg ggaagatgtc gatgttacgg tcgtcgcgga gttgcctgat
     1981 gaaatcaagg cgatgcaaaa ggggtatcgt tccgtagatc ccaagcaagc ggttgcgcag
     2041 atcgcggaag agtatgacga cacggcggaa atcgagatta tcgaggagag gttggtggat
     2101 ggtcttttgg tggaggagtt gctcaacgat gaggatactg ggcccaatgc gttgatgctt
     2161 cccatcggag aggtattgac ggacgtgggg cgtcgtcgtc cgaataacga agaccgtccc
     2221 ttgtctttgc cggagcattt tgtgttcggg gtagccgacg ggatgggggg gcatgcggcg
     2281 ggggaagtag cgagtaaaat cgctatggaa acgatcgaga aggcgtttga agacaacgta
     2341 ttcgaagggg agccgaactc gttgtggccg cgtcgtggcg atgaactggc gcggggcatt
     2401 gagatggcca atcggaatat tttccgtgtg gcgatggaag acgatgcgct gagtgggatg
     2461 ggaacgacgg ttacggccat tcgtttttgt ccggagcggc agcgggccta tattgcccat
     2521 gtgggtgatt ctcgttgtta tcgatttcgc gaagggcaga tgcggcagct aacgaaagat
     2581 cacacgctag gagcggaatt gggggcgaag ggcaagatgg cgacgcacct atccaggtcg
     2641 gtggggattg ccgagacggt ggaagtggat ctgatggtgg atgcgccgcg ggtgggggac
     2701 aggtatcttt tgtgtacgga tggcttaacg aaaatgcttt cagaagagcg cattctggag
     2761 atttccaccg atggcatcgt ggccgactgc gttcggcatc tcatcgacga ggcgaacgac
     2821 aacgggggga aggataatat tacggtggcg ctcgtggaga tccgagaacc agttggaaca
     2881 gggatgacgt aatggtattg aaacccgcat cgtgggttca tcgtgagcgt ttcggatctt
     2941 cgctactttc gtcatctcaa ttacagcctt ggtaacgagg acagcgcggt ggaatgggcg
     3001 gtgctgcccg agggtgtggg gcgagtattg tgtgttgccg gatgtggctc gcgggttctt
     3061 cccctgcttg ctcgcaagcc ggctcgggtg gtttgcgcgg atatatccgc ttcgcaactg
     3121 tggatgacgg aactgcgcgt ggaggcggca cgtgcattga accatgcgga atacttacag
     3181 ttttttggct acccgccgaa tcctggatcc ccggagcagc gtcgacggtt gtttcatcgc
     3241 ctggcactat cggacgaagc ccgagcgtat tggctcgatg tattcgacca tcgtgattgg
     3301 cagacggttt tatatgatgg gcgatgggag cggacatttc gaaagctatc gaggattaac
     3361 gcgatgttga cgggtgggcg gggccgcgca ttgttcgagg cgcgaacgag ggagcagcat
     3421 cgcgagtaca tgcgagagcg ctttcctcgt cgggcatggc tcatggtgct tttgttgttg
     3481 ggcaacccgc tcgtgttcaa tctcatgctt tataaggggg atttccctcg aaaaaatatc
     3541 gaggagactc ccttttcgtt ttattgtcga gcgttcgaca ggctgttttc gcttgggccg
     3601 gcgcgggaaa acttcttttt gcagatcgtg ttttgggggg aactcaggtt cgcggagggc
     3661 aatccgatcg aatgtgatcc tggtgttttc gccaagatcc aagaggctct tggcagcgcc
     3721 accatcgact atcggctggg cgatattttg gagatagcgc atcgggaagg gggccatttc
     3781 gacttcgtat cgttgtccga tgtgccttcc tatttcgtac cgccgcggga gcaaacgtac
     3841 ttgcaggact tgcgtccggc gttgtcctcg cgagcgcgtg tggtgcttcg ctattacctg
     3901 cgcagaccgg agcgggcgga aaaacgaggc tatggaaatc tgaccgagcg gtatgcgtcg
     3961 gcgatcgctg ctgagaaagt gggggtttac gatattgagg ttctcgagaa gctcgaggat
     4021 aataggtagg gatcgctgtt tctgtggggt tgatccacag cgggagcaga gacgaactgg
     4081 ttcaatgggg ggtgacggcg gacgaggctg gcgcgggggc gttggcgcat gggtggaggc
     4141 ggtcggggtg ttggagcatc caagacagag ctggaagagc cttgtccatg gctttcgtgc
     4201 tggtttccag gtccgtaaag gagccaggtg attcagcgat ttcttccacg atgatttcat
     4261 ccgaggcgtc gagcatcctc gagcgtgcga gtgtgtcgat ggtccacaag tgggtgtcag
     4321 accagggtgg gatgatggcg aaggtggggc tcatggcttg tccgccgagg gtatgccagc
     4381 gttgggaggg cgcgagggag cgcaatgcgc cgaagccatc gaggaaggcg acgagcatac
     4441 ggggaaggtc attttgtgac caaccatggg catcgagttc gcgttgtgcc gaagtgaccc
     4501 gtgctacatc ggtatcggat ggaaataccc atatgggagc tattcgactt cgttgaaggg
     4561 gattggtggt cgttccgaca ccgctggtgt ggtcgcccac catcatcacc acggcggtgc
     4621 gtccactttt ttcttcggct tgcatgaaag cggggatgaa gcgggataga gcatgatcca
     4681 ggtaagctac ggtcgtccaa tgagcgcgtt tcacgctgtc ttgtgtgctt ttgtgtacga
     4741 ggtcccgaga gcgatcccga aggtccgcgg gcatatcctg gggaatatcg aaggggccat
     4801 gggtcgaaag ggtcagcacg ccccggatcc gggtggcctg gggggtgatg ccagcggagg
     4861 tttgtgcaag atcagccaga acgtcggcaa agagttcttg gtctgacaga ccccacgcgc
     4921 ctcgggctcg tccttgtgtg cggggagtat gcaagtagcg gaaaccatgt tcgagaagcg
     4981 tctcgtcata gcgatcgaat cgaaggttat cggcgtaaaa aaagcgtaaa tccccgcctg
     5041 cctccgcgac gaggtcgggc caacagcgga gtttgagtcc tggaagatcg cgaagaggcg
     5101 cgataccgaa cggtccggta cccacgccgc acagcagcga tgacatggcg ccggcggttc
     5161 gttgtcctcc ttgatgaaaa cgctttccta caagcacatg gggtgtgcgt tggatcaagt
     5221 cgtcgagata aggggtcagg ccatggggag cgcctgcatc caagcaggca atgtcttctg
     5281 cacccacgct ttcgagcatg atgaagagca gggataacgg gcgtccgcgt ccacccaaat
     5341 gtatccccaa ttcgtataac ctattgtaaa ctaacgtgtt ttttgatgat tgggtgattg
     5401 gctccgccac gtggaggccg tcgggacagt gttggatcgg cagaccgatc atggcgagag
     5461 cgggtagggc ggaggagtcg gtgacgtttt gttttttcaa tgcaggttcg aggccgacga
     5521 tgatgttgag gtgattggtc gtgaccaggg ctccaagcaa gcggtcatag gggcgcaggg
     5581 cgtgtttgcc tcgatccgtg cggtgaattc cgagcgatag gaggatgaaa gttccgagca
     5641 cggcgagcac gatcaggatt ccttgtcgtg ggatcgggat ccattggcga tgcagcaggc
     5701 tcaggctgag ggcgaaaacc gcgatggaca gggccaggta ggggccaaca tgcgatgagg
     5761 tcagcacacc gagttggctg ttgagaaaac cagcgtccag cccgttgagc acatcgatgg
     5821 gggcgatggg agccccgcgt tgcagcataa attcggtgca ggtgatggtg gtgatggttt
     5881 gcacgacgaa caagagccct aggctgatga aaaaaagggc ttttcgtttg tgaagccaaa
     5941 cgaagagcaa ggtcgtgaaa agcagaccgg gggtaatatt gccgaacgcg ccggtgagag
     6001 cgacgatcca tctgaattga ccggcgatgc accaagcgag ggcggcggac aggatggccg
     6061 cgatgttgat ggtggtgagg agcaaaccaa tggtgagaaa ggcaggccaa ggggacggct
     6121 ggctggtggg ggacggcatc aagtctttgg aaggcgaaat cgacagcaag atacggcaga
     6181 ctccacggtt tcgttgggcc cttgatggga agcccggaca ttcatcgtca acgacaacct
     6241 tggcgtgtac gataggagca attacgtgta gcgggtatgg gaatgcaaga caacacgctg
     6301 ctttatgatg tttgcgattt gagtttcgtc tatgagcttg ggagacagaa agtccatgcg
     6361 cttcgcaacg tatcgcttca ggtgaagcgg ggtgatttcg tatgccttgc cgggccctcg
     6421 gggtcgggga aaacgacctt gctcagtgtg cttgggctga ttgagcctct tcagcaaggt
     6481 acggtgcgtt tcgagggaag aagtttgtcg gagatgacgg agacggatcg aaaccgtttg
     6541 cgtcgcttcc acattggttt tgtgtttcag caatttcatc tcataccggt tctttcggcg
     6601 gaagagaacg tcgaatactt tttgacaagg cagggcgtgc caaaagcgga gcgtcgcaaa
     6661 cgtgtggacg aagccttgga gaccgtggga atttcatcgc aacgcaagca gcgtccgttg
     6721 gagatgagcg gaggtcagcg gcagcgtgtg gcgctggctc gtgccttggc caaacgtccc
     6781 aatgtcatca ttgccgatga gcccacggcg agtttggatc aagcgacggg caaagagatc
     6841 atgcgaattt ttgcggagca gacccgagaa cggggggtga gtgtgattgc agccagtcac
     6901 gacccgatgg tgtttgcgtt tgcggctcgg caaatcgaac tacgagatgg ggaggtcgtg
     6961 tcatgctaat tttggtcgcg ctgcgcaatc tgatgcgtca tccccgtcga acgtttgcgg
     7021 tg
//
LOCUS       DAOSOR010000296         7012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10793_length_7012_cov_4.094581, whole genome shotgun sequence.
ACCESSION   DAOSOR010000296 DAOSOR010000000
VERSION     DAOSOR010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7012
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10793_length_7012_cov_4.094581"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..721
                     /locus_tag="PK710_15215"
     CDS             200..721
                     /locus_tag="PK710_15215"
                     /inference="COORDINATES: protein motif:HMM:NF024645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT11128.1"
                     /translation="MKYVLSSIGVVLGTILLLLGLLFLVGFGGHFYRLVIAALGITLG
                     SISIGAGIWGWKRAQAHSPAQVRKDLLALAGRKSGRLTEADVVAAMPDRAQAAVDALH
                     ALVRAGHAVHAPTQGVMYFEFPDLRPTISIRRCSHCGWESPLSSQATVCAQCGGPLTL
                     GQTTDDDAIGLDM"
     gene            877..1986
                     /locus_tag="PK710_15220"
     CDS             877..1986
                     /locus_tag="PK710_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11129.1"
                     /translation="MIHLRRLMYFWFCGFLFGCATTPSLSTPPHASKTENSVTPDPRP
                     TSLPHPVENHDPPPPEPTQSVQTVVLRDFQRILEVPVRSIAVGKPPHVAVLADQTYWY
                     DGNDWHSQPFPSPHALSDRDRVEIFFGRDNRPRLMGSATTGDTTHAVYYRWKTAGWRK
                     EPSEIGKLAGAPSGGLFGVLGDEDPEVVCKVSDICLIKRRTGWSTFASGQGMPRVMLF
                     HGFVAALHPDHLSILEENGWRDLPGKAPFSNATGFWGQRNALWVSEAQENSLFFWNGE
                     KWAPGASPIDGPKALWGTAPNDVWLVGQGGAGHFDGSTWHRVAGLDGPLEVVTGSAQG
                     DVWIGGPSGLWRGKNQESVASAHVDAARRSPPEIR"
     gene            complement(1937..3214)
                     /locus_tag="PK710_15225"
     CDS             complement(1937..3214)
                     /locus_tag="PK710_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOT11130.1"
                     /translation="MSRSKVALLRTSPATVLEDYHRLMNLAGYQDVIAKDADTGLKIN
                     ISWHFFYPAASTTPWQLDGVIRAMKRDGYDPDLIHGCHNRTVVIDAHLGERENKQVDV
                     INAHGLRNVHLYEGEDWIHIRDAVGDLADDFLVLNDVYKKGFSIPKRFIGENIIHLPT
                     VKTHIFTTTTGAMKNAFGGLLNEHRHWTHPVIHETLVDLLMIQKKIHRGVFAVMDGTF
                     VGDGPGPRCMVPHVKNVILASQDQVAIDAVAAKLMGFDPMRDLKFVRIAHELGLGCGD
                     PRDIEIVGDQEAAEMNWHFDGPFKNMTFASRNQHRIYWGPLKKPLEWSLKTWLAPWSY
                     VASVSYHDMFWYPMYGKTRVNDALMSEWGRLFANWNQVQPDPEAKGFPDVGTENPELV
                     RLGIKHFLEGLRLVGMAVTESPEVSAWQRQHAR"
     gene            complement(3389..4321)
                     /locus_tag="PK710_15230"
     CDS             complement(3389..4321)
                     /locus_tag="PK710_15230"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT11131.1"
                     /translation="MTKKVLLATEKPFAPAARDKVVAVLKEARYEVVLLESYTDKAQL
                     LDAVKDVDAMIIRSDKVDPAVLDAAKNLKLVVRAGAGYDNIDCAYAKQKGVFAMNTPG
                     QNSNAVAELVFGMMLMMARGRFSGKSGTELKGKTIGIHAVGNVGRCVARIAKGFDMTV
                     LGYHPRRTAEVIRAAGAEPVDSAETLYAKSEYISLHVPATPENKQSIGKALLMSMPKN
                     ACVINTARKEVIHEPELLEVFEARPDFRYATDVEPSAEVAKVLQEKYADRFFMTPKKM
                     GAQTSEANVNAGIAAAEQIVAFFEKNDRQFIVNA"
     gene            complement(4425..4649)
                     /locus_tag="PK710_15235"
     CDS             complement(4425..4649)
                     /locus_tag="PK710_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11132.1"
                     /translation="MGKKESTVENRVYLFRDLAGAWLASFGDQLGAEDEKIRQRGRDA
                     LAEIAYISCVLSDTETLDAKSVSKLIRKGS"
     gene            complement(4668..5678)
                     /locus_tag="PK710_15240"
     CDS             complement(4668..5678)
                     /locus_tag="PK710_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11133.1"
                     /translation="MSWRMNSGLRWILLLSPMAMIHCGTSLPPSKIVDSKPVSGSAAV
                     GDRDDSANVCPEVPIVSMVLRISTPNGSRWMVHERQASRQDEFFPGVAEEMARPMTEG
                     EAKQNGMASVGSTVWVYTNDEAPPCGMQAGAPWAVHYGDGALRTHLFMELSGQCPLDD
                     QAPSYLAIRGVQEARGCRMHSLPVMAADEQGVGSTVMPADVRKLLPDNHCEEPKCFRW
                     IALGGHVDGGAGIYEVGAVWIHPVEGDEPACAAPFDGFHGVYFQPTSNAPWQEIPEVT
                     GVRSMFFDRRGLRGIVTDAEGVLEVFVVGPNGLQKVSEQRYMSWNEENRGRIEEIQPS
                     CF"
     gene            complement(5696..6133)
                     /locus_tag="PK710_15245"
     CDS             complement(5696..6133)
                     /locus_tag="PK710_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11134.1"
                     /translation="MERTRQELAQARMGVDQLLQDCERLNAENVRLKAAMKRRERTSG
                     FPQDEPSKQESAREQPETSESRRSSRAAPAPQSEEAFRTAPTRRSMRTTRRSKAKGME
                     EPVSTPQADSENIKEKRTVGSYFLTGDEVSEETIPERPKRRGR"
     gene            complement(6188..>7012)
                     /locus_tag="PK710_15250"
     CDS             complement(6188..>7012)
                     /locus_tag="PK710_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11135.1"
                     /translation="VLDQERDRLRSDAEAGKKRMKEADARIVEAKNAQRKADEQLNET
                     LETLTTLGLAKAELERKVMDLEAAFEAVREERAGAEERWNATDEQRLSQIRLLRKNVE
                     ELERKLSGQDQERLSLMDSLKLSQQAVEQARAEIESQREKLSVQRSESKDRIEVLERQ
                     QEEFIATLVDDYDNQLGELRAQLEEVRNNTVERTRLQQLEQRHEEALKQLGNERDDLA
                     RQLEEVRKNTVERAQLQQLEERHEEALKQLQNERNGSVVPSESEGQLAELRRELDA"
BASE COUNT         1456 a         2199 c         1751 g         1606 t
ORIGIN      
        1 gctcaaagac cggtttgctt gagcgtcagt ttgaccgggt gatcaatcac gccctgataa
       61 tgcttctcac cccatcgaat ttgcacaaat ggtaaagttc gccacggaaa cgtatagtga
      121 aaccaatcta caatcgtttt tcaaccagat gggcgaagag ctatcacggc aagccaaatc
      181 gcaatgggga tgagaactca tgaagtacgt actgtcgtcg attggcgttg ttctcggaac
      241 gatcctgctc ctgctcgggt tgctatttct ggtgggattt ggaggacact tctatcgtct
      301 ggtgattgct gccctcggca tcaccctcgg ctcgatctcg attggcgcgg ggatatgggg
      361 gtggaaacgt gcgcaagcgc attcccccgc acaagtacga aaagacctgt tggctctcgc
      421 ggggcgaaaa tcgggaagac tcaccgaagc cgatgtcgtg gccgcgatgc cagaccgcgc
      481 ccaggccgcc gtcgacgcgc ttcacgcgct cgttcgcgcc ggacatgccg ttcacgcccc
      541 aacgcaaggc gtaatgtatt tcgaatttcc ggaccttcgc cccaccatct ccatccgccg
      601 atgctcccat tgcggctggg aatcacccct gtcttctcaa gccaccgttt gcgcacaatg
      661 cggaggtccc ctgaccctgg gacaaacgac agacgacgac gccatcggac tcgacatgta
      721 gcccgaatcc aatcagcgtc gcccctgccc ccgatgcttc aaccttcgac ctcgatggaa
      781 acatatacaa ggccccatag acacgtaacc ccatgaaata acgatagaaa tagctcctac
      841 cccggttcct ctctctcacc ccaatagcga gttttcatga tccatctacg acggcttatg
      901 tatttttggt tctgcggatt tttgtttggc tgcgccacga ccccttccct ttccacaccg
      961 ccgcacgctt caaaaacgga aaattccgta accccagacc cacggccgac ttcccttccc
     1021 caccccgtgg aaaaccatga tccaccccct cccgagccga cccaatcggt acaaaccgtt
     1081 gtgctgcggg acttccagcg gatcctggag gtacctgtcc gctccatcgc cgtgggaaag
     1141 ccgccgcatg tagcggtgct ggcggatcaa acgtattggt acgatggaaa tgactggcat
     1201 tcccaacctt ttccgtcgcc ccacgccctg tcagaccgtg accgggtcga aatatttttc
     1261 ggcagggata atcgccctcg cctgatgggt tccgccacga ccggggatac gacccacgcc
     1321 gtatattatc gttggaaaac ggcaggatgg aggaaggagc cctcggagat tggaaaactc
     1381 gccggtgctc cttcaggtgg gttatttggt gtcctgggcg atgaagaccc cgaagtggtc
     1441 tgcaaagtat ctgatatctg cctcatcaaa cgacgaacgg gatggtccac ctttgcttcc
     1501 ggccaaggca tgccccgggt gatgctattt cacggtttcg tagcggcgct gcaccccgac
     1561 catctatcca tcctggaaga aaatggctgg cgggatctgc cgggaaaagc gcctttttcg
     1621 aacgccacag ggttctgggg ccaacgcaat gcgctgtggg taagcgaagc gcaagaaaac
     1681 tccctatttt tttggaatgg cgagaagtgg gcccctggcg cttccccgat cgacggtccg
     1741 aaagccctat ggggaacagc ccccaacgat gtttggttgg tgggacaggg aggcgcgggc
     1801 cacttcgatg gttcgacctg gcaccgtgtt gctggcctcg atggccccct cgaggtggtg
     1861 acaggctcgg cacaaggcga tgtttggata gggggaccgt cgggattatg gcgaggaaaa
     1921 aaccaagaaa gcgtcgctag cgcgcatgtt gacgctgcca ggcgctcacc tccggagatt
     1981 cggtgacggc catgccgacg agccgcaacc cttcgagaaa atgcttgatt ccaagacgaa
     2041 cgagttccgg attctccgtg cccacgtccg ggaatccctt tgcctccgga tccggttgga
     2101 cctgattcca attggcaaac aaccttcccc attcgctcat gagagcgtca ttgacacggg
     2161 tttttccgta catcggatac cagaacatgt cgtggtacga cacggaagcg acataagacc
     2221 acggtgccaa ccaggttttt agcgaccatt cgagcggttt cttgaggggc ccccagtaaa
     2281 tccggtgctg attacgcgat gcgaacgtca tgttcttgaa cggaccatcg aagtgccagt
     2341 tcatttccgc cgcctcttga tccccaacga tctcgatatc tcgtgggtca ccacatccga
     2401 gtcccaactc atgagcgatc cgaacgaact tcagatcccg catcgggtcg aaccccatga
     2461 gtttggccgc taccgcatcg atggccactt ggtcttgcga cgccaaaatg acgtttttga
     2521 catgagggac catgcagcgg ggccccggac catctcctac gaacgtgcca tccatgacgg
     2581 caaacacacc gcgatggatt tttttctgga tcatcaacag gtccacgaga gtttcgtgga
     2641 tgacaggatg agtccaatgt cgatgctcgt tgagcaagcc gccgaacgcg tttttcatcg
     2701 cgcccgtggt cgtcgtaaaa atatgcgttt ttacggtcgg caagtggatg atgttctcgc
     2761 caatgaaccg tttgggaatg gagaatccct ttttgtatac atcgttgaga acgaggaagt
     2821 catccgccag atcgcccacg gcatctcgga tgtgaatcca gtcttctccc tcatatagat
     2881 gaacgttgcg caagccatgc gcgttgatca catcgacttg cttattttcc cgttccccaa
     2941 ggtgcgcgtc gatgaccacc gtgcggttat ggcaaccatg gatgaggtcg ggatcgtaac
     3001 catctcgctt catcgcacgg atgaccccat cgagttgcca cggtgtggtg gaggcggcgg
     3061 gatagaaaaa atgccaggag atattgattt tcaggcccgt gtccgcgtct ttggcaatca
     3121 cgtcctgata gcccgcaagg ttcatcagtc ggtggtaatc ctcgagcacc gtcgcagggc
     3181 tagtacgaag aagggcgact ttggatcggc tcatggtaga acaagcgctc cttagttcgg
     3241 tgaataagac aatttccgtg tgtagcaagc ccttggcttg ggaagcaaga gcgggttatt
     3301 cattcaccaa aggaaaaacg attctgctga tggcaaaatc aggtgtggac ttcctacgat
     3361 cgaaaagcgc gtcgcttcga gcaaacgcct aggcgttgac gatgaattgc ctgtcgtttt
     3421 tctcgaagaa agcaacgatt tgttcagccg cggcaatgcc cgcgttcaca ttcgcttccg
     3481 aggtttgcgc ccccattttc ttcggtgtca tgaagaaacg atcagcgtat ttttcttgaa
     3541 gaacttttgc cacctccgcg gaaggttcga cgtcggtggc ataccggaag tcaggacgag
     3601 cttcgaatac ctcgagcaat tccggctcat ggatgacttc tttgcgagca gtgttgatca
     3661 cacaggcgtt cttgggcatc gacatgagca gcgccttgcc gatgctctgc ttgttctccg
     3721 gcgtggcagg aacatgcaaa gaaatatatt cggacttggc atacagggtt tccgcggagt
     3781 cgacaggctc ggcccccgcg gcccgaatga cctccgccgt tcgacgcggg tggtatccga
     3841 gaacggtcat atcgaaacct ttggcaatgc gagccacaca acgccctacg ttgccaaccg
     3901 catgaatgcc gatcgttttc cccttgagtt cggtaccaga cttaccgctg aaccgaccac
     3961 gcgccatcat cagcatcatt ccaaacacca actcggcgac ggcattggag ttttgtccag
     4021 gcgtattcat ggcgaatacg cccttttgtt ttgcgtaggc acaatcgatg ttgtcgtagc
     4081 cagcgcccgc gcgcaccacg agcttgaggt ttttcgcggc atcgaggaca gcaggatcca
     4141 ccttgtccga gcggatgatc atcgcatcca catctttgac cgcatcgagc agttgcgcct
     4201 tgtccgtgta ggactcgagc aatacgacct cgtagcgcgc ctccttcaac acggcgacca
     4261 ccttgtcacg cgccgccggc gcaaaaggct tctctgtagc aagtaggacc ttcttcgtca
     4321 tgacgcacct caacaatccg atagggttga tgttcgacca gtggcatggg tcctaaccct
     4381 tttacgcaag cctcacaacc cccaccagcc caccgagcgc cccgtcatga tcccttgcga
     4441 atgagcttgg acacgctttt ggcatccaac gtctccgtat cggataacac gcatgaaata
     4501 taggcaattt ctgccaacgc atcgcgcccc cgttgcctga tcttctcgtc ttccgcaccc
     4561 aattggtcgc caaacgatgc gagccatgcc cccgccaaat cacgaaatag gtacacccgg
     4621 ttctccaccg tcgattcttt ctttcccatg atctttctcc gaatcgctta aaaacacgaa
     4681 ggctggattt cttcgattcg acctcggttt tcctcgttcc acgacatata tcgctgctca
     4741 cttaccttct gcagcccatt cggcccaacg acaaatacct ccagcactcc ttccgcatcc
     4801 gtgacgattc cacgcaatcc acgacgatcg aaaaacatcg accgcacccc cgtcacttcc
     4861 gggatttctt gccacggagc attgctagtc ggctggaaat acacgccatg aaacccatcg
     4921 aaaggcgccg cacacgccgg ctcgtctcct tcgaccggat gaatccaaac ggcccccacc
     4981 tcgtaaatcc ccgcaccccc atccacatgc cctccaagcg caatccaacg aaaacacttt
     5041 ggctcttcgc aatgattgtc cggaagcagc tttctgacat ccgcaggcat caccgtcgac
     5101 ccaacgccct gctcatccgc cgccatcacc ggcagcgaat gcatccggca ccctcgcgcc
     5161 tcctgcacgc cacgtatggc aagatagctc ggcgcttgat cgtccaaagg acattgcccc
     5221 gaaagctcca tgaatagatg tgttcgaagg gcgccatccc cataatgcac cgcccatggc
     5281 gcacccgctt gcatcccaca gggaggcgct tcgtcattcg tatacaccca taccgtcgat
     5341 cccaccgacg ccatcccgtt ttgcttggcc tccccttccg tcatcggccg cgccatctcc
     5401 tccgccaccc cagggaaaaa ctcgtcctga cgagacgctt gtcgctcgtg gaccatccag
     5461 cggctgccgt tgggtgtcga aatacgcagc accatcgaca cgataggcac ctctggacat
     5521 acgttcgccg aatcatcccg atcgcccacc gcagccgagc ccgatacggg tttcgaatct
     5581 actattttcg aaggaggcaa cgacgttcca caatgaatca tcgccatggg cgagagcaaa
     5641 agaatccacc gtaaacccga attcattctc cacgacatca cgctcaccct tgccttcaac
     5701 gcccgcgccg cttgggtcgt tcgggtatcg tctcttccga gacctcgtca cccgtcagaa
     5761 aatagctccc aactgttcgt ttttctttga tattttcgct atccgcttgc ggtgtggaaa
     5821 caggctcctc catgcctttc gccttcgacc gtcgcgtcgt gcgcatcgac cgccgggtcg
     5881 gcgccgttcg aaacgcttcc tccgattgcg gtgcgggcgc cgcacgcgaa gaccttcgag
     5941 actccgaggt ttccggctgt tctcgcgcgg attcctgttt cgaaggctcg tcctgcggaa
     6001 acccggatgt ccgctcccgt cgcttcatcg ctgctttcag acgaacattc tccgcgttga
     6061 gacgctcaca atcctgcaga agctgatcga cccccatgcg agcctgcgcc aactcctgac
     6121 gcgttcgctc caactccgcc gccagttctc tcccatcctg cgaagtctcg ctaccactct
     6181 gggcacgcta tgcgtcgagt tcacgtcgca gttcggccaa ttgtccctcg ctttcggatg
     6241 gtacaaccga gccgttcctt tcgttctgaa gctgcttcag cgcttcttcg tgccgctcct
     6301 cgagctgctg caactgcgct cgctccaccg tgtttttccg aacctcttcg agctgccgcg
     6361 ccaagtcgtc tcgctcgttt ccaagctgct tcagcgcctc ttcgtgccgc tgctcgagct
     6421 gctgcaaccg cgttcgctcc accgtattgt tccgaacctc ttcgagttgt gcccgtagct
     6481 cgccaagctg gttgtcataa tcatccacca gcgtcgcaat gaattcctct tgctgtcgtt
     6541 cgaggacttc gatccggtct ttgctttcgg accgttgaac agaaagcttt tcccgctggc
     6601 tttcgatttc cgcgcgagct tgttcgactg cctgttgact aagtttcaac gaatccatga
     6661 gcgaaagacg ctcttgatct tgaccggaaa gctttcgttc caattcctcg acattcttac
     6721 gcagcagacg aatctgcgac aaacgctgct catcggtggc gttccaccgc tcctcagccc
     6781 cggcacgctc ttcgcgaacg gcctcgaaag cagcttcgag atccatgact ttccgttcga
     6841 gttcagcctt cgcaagccct aacgtcgtca gtgtttccaa cgtctcattg agttgctcat
     6901 ccgccttccg ttgggcattt tttgcctcga cgattcgggc atcggcttct ttcattcgct
     6961 tttttccggc ttctgcatcg ctgcgcagcc ggtctcgctc ttgatcgagc ac
//
LOCUS       DAOSOR010000297         7009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10799_length_7009_cov_4.355335, whole genome shotgun sequence.
ACCESSION   DAOSOR010000297 DAOSOR010000000
VERSION     DAOSOR010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 7009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7009
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10799_length_7009_cov_4.355335"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PK710_15255"
     CDS             complement(<1..333)
                     /locus_tag="PK710_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11136.1"
                     /translation="MGDGEDKVSIDQPEQMNLLGLLLAGFLRKQLTHPRMARKAARIR
                     GAYGIRAADMAITLTFTPETIRISKGFSKKTRARIFGSMEEMIALVAGSGSTIAAIIA
                     VLEGRIHIR"
     gene            512..1579
                     /gene="hydE"
                     /locus_tag="PK710_15260"
     CDS             512..1579
                     /gene="hydE"
                     /locus_tag="PK710_15260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOT11137.1"
                     /translation="MPIVTPLDRGTILTWLREKDPSRLQSLWQAADETRRSHVGDEVH
                     LRGLVEISNYCRRQCAYCGIRAGNRGVVRYRMTQREIVDCARHAKSLGFGTVVLQAGE
                     DMGLTGAWVSELLRAIKSETALAITLSLGERTSEELAAWKQAGADRYLLRFETSDPEL
                     FRKIHPSLSSTAVSDRMAQLRVLRTLGYEVGSGVMVGIPGQTWDSLAQDILTFQSLDL
                     DMIGVGPYIPHPDTPLGVSSEDAGQEQVPSSEQVVYRVLALTRLMCPEANIPSTTALA
                     AVNKKQGRQLGLMRGANVIMPNLTPDAYRISYAIYPDKACLNETMQVGPSAILQQLAS
                     WGRKVGQGHGGRRRKAQGVRE"
     gene            complement(1604..2866)
                     /gene="ftsZ"
                     /locus_tag="PK710_15265"
     CDS             complement(1604..2866)
                     /gene="ftsZ"
                     /locus_tag="PK710_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOT11138.1"
                     /translation="MTFSIEFADDSNNEYQANIKVVGIGGSGGNAVNTMISFGLEGVE
                     FIVANTDAQALNASAAPVKLPIGQTITRGLGAGANPEKGRKAALEDVTQIKQFLQGAD
                     MVFITAGMGGGTGTGAAPIIAQIAREEGALCVGVVTRPFAFEGKQRARIAEAGLNELA
                     SQVDSLITIPNQKLLTFSDEDLSFTEAFRRADEVLYQAVKGISDLIQLNGVVNVDFAD
                     VKTIMSNRGRAIMGTGCAKGDGRAALATHMAISSPLLDNISVEGATAVLVNIVGGSTL
                     KIKEMEEATEIVYEQAHEDANIIWGASVDESMGDMIKVTVIATGFGQYAEEVVREQES
                     QRPTMVAVPQQVATSRVAPQGMPSRAAIDTRFHPAASTSSRAPAPASSAPAPAPVSRH
                     PRSTFAPNADLDWDAPAFSRHSGEYPPR"
     gene            complement(2989..4290)
                     /gene="ftsA"
                     /locus_tag="PK710_15270"
     CDS             complement(2989..4290)
                     /gene="ftsA"
                     /locus_tag="PK710_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOT11139.1"
                     /translation="MVVGCTSAVRHLGEESMESSEIVVGLDIGTTKVAAVVGEVDRDG
                     ITIMGVGNVPCKGLRKGVVANIEWTMKSITEAVQMAQSMAAVEIRTVYAGVGGSHIRS
                     QNSDGVVAVAGGEVTAADVDRVLEGARAVAMDADRQILHVLPREFLVDNQDGIRDPVG
                     MSGVRLGTKVNLTTAATTCVQNVVRCTERCGLTVADVVLAPLASAEAVLSEDEKEIGV
                     AVIDIGGGTTDMLLYVDGGVAYTSVIPAGGNNVTSDISMGLRTPMAEAERLKRNAGCA
                     LGRMVSDEEEIEVPGVGGHTARKVARRVLSDIIEPRIEEIFAFARHRMEQVGLLEQIA
                     AGAVLTGGAALMEGMPEFAEEILGMPVRIGFPIGISGLTQMVQGPQFATGVGLVRHGA
                     AMLREAQDREYSRVAPRVEQEEAVIVKREGNRIWQWLREAF"
     gene            complement(4432..5376)
                     /locus_tag="PK710_15275"
     CDS             complement(4432..5376)
                     /locus_tag="PK710_15275"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOT11140.1"
                     /translation="MIGRKRSSTTEEPSRPSVLPSNRRVAPAEGQASAASSRNAGNEG
                     KGSTKKPRAGRLRSWAGGFRARLSGLRAAASIVFVVASSIALAWGIKQHVVSSPRFAV
                     KTIRIDGAVRRSPEQISEAAGLRVGMNVFSVDLEAARNRLLLDPWVATAQVERQLPAT
                     LTITLTEREAAAVVAVDADLYLCTPEGELFKKLEADDPIGLIVITGLTSSLVANDRKL
                     AVSRVRSALELMAQYASRGPVQQHPIQEIGIADDGSLRMVVGRSAITLEMGRSPYPRK
                     VLQAARVIQEVQKKQKGDPAIVFLDNEAHPERVVVRMR"
     gene            complement(5390..6784)
                     /gene="murC"
                     /locus_tag="PK710_15280"
     CDS             complement(5390..6784)
                     /gene="murC"
                     /locus_tag="PK710_15280"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOT11141.1"
                     /translation="MFRGRVRQIHFVGIGGIGMSGLAEIVHALGFEVSGSDLKPNSTT
                     QRLDALGVKIHIGHRAANIVGSDVVVYSSAIKPDNPELVEALTSGIPVIARAELLAEL
                     MRVKYGVAVAGSHGKTTTTSLVATVLRAAGFDPTVAVGGKMAALGSNAKLGAGDLMVA
                     EADESDGSFLLLSPTIAVVTNIDPEHLDYWGSHERIKEAFIQFCSRVPFYGLAILCLD
                     HPDVQSILPAVRRRHTTYGFSPQADYRAKDVKAVGTEMRFKVLKQGELLGNYSVRMPG
                     RHNVLNCLATIAVADELAVPLDVIQQSLADFEGVARRFTVVGEAKGVTLIDDYGHHPA
                     EIRATLEAARAGYDRRLVVVFQPHRYTRTRDLFEQFVTSFNLADVVVVTEIYPAGETP
                     IAGVSGQLLAKALREHGHQRVHYVQNKDNIPDFLQKLVLRGDVVIAQGAGDINQSIRE
                     LGRRLEAEDGADEG"
     gene            complement(6784..>7009)
                     /locus_tag="PK710_15285"
     CDS             complement(6784..>7009)
                     /locus_tag="PK710_15285"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT11142.1"
                     /translation="GDHQLHNARALESDGAAICIPSSDASPTVLADHLVRLANDEKAR
                     RRMADRARAHGRSEAAAAIADDLLHLAEVL"
BASE COUNT         1443 a         2196 c         1926 g         1444 t
ORIGIN      
        1 acggatgtga attcgccctt cgagcacggc gatgattgcg gcgatggtgc tgcccgagcc
       61 tgccaccaac gcgatcattt cctccatgct gccgaagatc cgggcgcggg tctttttaga
      121 aaaacctttt gaaattcgaa tagtttccgg agtgaaggtg agcgtgatgg ccatgtctgc
      181 cgcgcgaatt ccgtaggccc ctcggatgcg agctgccttg cgagccattc tgggatgggt
      241 gagttgtttt cgaagaaaac cagccaagag cagacccagc agattcattt gctcaggttg
      301 gtcaatggat accttgtctt cgccatcacc cacggaaacg cctccgacgt accgaaccga
      361 aacggaactc taagggcaat cgaagcgcgt gtcacgctag gtgaagagcc atgccctcat
      421 cttcacgagt tcatcggcaa acacgccatc ggagcacggt acatcgggcc tgtccttgac
      481 gaaacggaca agcaagagaa aggtccggtc aatgccaatc gtaacgccgc tcgatcgcgg
      541 gaccatcctg acctggctgc gggaaaagga cccgtcacgt ctgcagtctt tgtggcaagc
      601 cgccgatgaa actcgccgca gccacgtggg cgatgaagtg catctgcggg gattggtgga
      661 gatctcgaac tactgtcgtc ggcaatgcgc ctactgtggg attcgcgcgg gcaatcgggg
      721 ggtggttcgg tatcgcatga cacaacgaga gatcgtggat tgtgcccggc acgcgaagtc
      781 gttgggattt gggaccgtgg ttttgcaagc aggcgaagac atggggttga cgggcgcatg
      841 ggtgtcggag cttttgcgcg cgatcaaaag tgaaacggca ttggcgatca cgttgagctt
      901 gggagagcga acgagcgagg aattggcggc gtggaagcag gcgggtgcgg atcggtattt
      961 attgcgtttc gagacctccg acccggagct ttttcggaag attcacccgt cgttgtcgtc
     1021 gacagcggtt tcggatcgga tggcgcaatt gcgcgtcctt cgaacccttg gctatgaggt
     1081 gggcagcggt gtgatggtgg gcataccagg ccaaacgtgg gattcgttgg ctcaggacat
     1141 ccttacgttt caatccctcg atctggacat gatcggggtg gggccataca taccgcatcc
     1201 ggataccccg ttgggagtat catcggagga tgcggggcaa gaacaggtac cgtcgagtga
     1261 gcaggtagtc tatcgtgtgc ttgcgctgac gcgccttatg tgtcctgaag ccaatatacc
     1321 gagtacgaca gcacttgcag cggtcaacaa gaagcaaggt cgtcaactgg gattgatgcg
     1381 aggcgccaac gtgatcatgc ccaatttgac tccggatgcc tatcgaatca gctatgcgat
     1441 ctatccggat aaggcttgtt tgaacgagac gatgcaggtg ggtccgtcgg caatcttgca
     1501 gcagcttgca tcatggggac gcaaggtggg acaagggcat ggggggcgac gtcggaaggc
     1561 acaaggcgtg cgggaatgac aagggaacgg tagcggagtc gaattatcgg gggggatatt
     1621 cgccgctatg gcgcgagaag gcgggggcgt cccaatccag gtcggcattg ggggcgaaag
     1681 tgcttctcgg atgacgagaa acgggagccg gagcaggggc acttgacgca ggagccggtg
     1741 cgcgggaaga agtagaagcc gcgggatgaa aacgcgtatc gatggctgct cgggagggca
     1801 tgccttgagg cgccacacgc gaggtggcca cctgctgggg cacagcaacc atcgtcggcc
     1861 gctggctttc ctgctctcgg accacctctt cggcgtattg tccaaagccg gtggcaatga
     1921 cggtgacctt gatcatatcg cccatgcttt cgtccacgga agcgccccag atgatattgg
     1981 catcttcatg ggcctgttca tagacaattt ccgttgcctc ttccatttcc tttattttca
     2041 atgtcgaacc acccacgata ttgacgagca ccgcggtagc tccctcgacc gagatattgt
     2101 cgagcaaagg agaagagata gccatgtggg tggcgagggc agcccggccg tcgcctttcg
     2161 cacagccggt ccccatgatg gcgcgaccac ggttgctcat gatggttttc acgtcggcga
     2221 agtccacgtt cacgacccca ttgagctgaa tcaggtcact gattcccttg acggcctgat
     2281 aaagaacctc gtcggccctt cggaacgctt cggtgaagga aaggtcctca tcgctgaaag
     2341 tgagtagttt ttgattgggg atggtgatga gactgtccac ctggctggcg agttcgttga
     2401 ggcccgcctc ggcgattctg gcgcgctgtt ttccttcgaa ggcgaagggt ctggtgacga
     2461 cacccacaca aagggcccct tcttctcttg cgatttgcgc gatgatggga gcggctccgg
     2521 ttcccgtccc tccccccatg ccggcggtga tgaacaccat gtcagcaccc tggaggaatt
     2581 gtttgatttg ggtaacgtct tccaacgctg ctttgcggcc tttttccggg ttggcccccg
     2641 cacccagccc ccgcgtgatc gtttgtccga tgggaagttt gacgggcgcc gcgctcgcgt
     2701 tgagcgcctg ggcatcggta ttggccacga tgaactcgac cccttcgagg ccgaagctga
     2761 tcatcgtatt gacggcattg ccaccagaac cacctatacc tacaaccttg atattggctt
     2821 gatattcgtt gttggaatcg tcggcaaact cgattgagaa ggtcatcacg tcctccggat
     2881 gcggcacggc cgctcatgtc cacgcacttc tttgccgacc ctcaggcgcc tctccgttgg
     2941 gtcggagcct ttcccacctt ctgctccgta ttcacgaagc gtcactcatc agaaagcttc
     3001 tcgaagccac tgccagatgc ggtttccctc tcgtttcact atcaccgctt cctcttgctc
     3061 gacccgagga gccacgcgcg aatactcgcg gtcttgcgct tcccgcagca tcgccgcccc
     3121 atgccgcacc aggccaacac ccgtggcgaa ttgaggccct tgaaccatct gcgtcagacc
     3181 cgatatgcca atgggaaatc caattcgtac tggcattcca aggatttcct cggcaaattc
     3241 tggcattcct tccatgagcg cggcgccgcc cgtcagcacg gctcccgccg caatctgctc
     3301 caatagcccg acctgctcca tccgatgccg ggcaaacgca aaaatctctt caatccgcgg
     3361 ctcgatgatg tccgataaca cccgtcgcgc taccttgcgc gccgtatgtc cacccacacc
     3421 aggaacctca atctcttcct cgtcggaaac catccgcccc aacgcacatc cagcatttct
     3481 ctttagacgc tccgcttccg ccatcggcgt gcgaagcccc atcgaaatat cactggtcac
     3541 gttgttgccc cccgccggaa tcacgctcgt atacgcaaca ccaccatcga cgtacaagag
     3601 catgtcggtc gttccccccc caatatcgat caccgccacg ccgatttcct tttcatcctc
     3661 actgagcacc gcctcggcac tcgccaaagg cgcaagaacg acatctgcca cggtaagacc
     3721 acagcgctcc gtacaacgaa ccacgttctg cacgcatgtg gtggccgcgg tggtcaagtt
     3781 gactttggtg ccaagacgaa ccccgctcat gcccacggga tcacgaatgc cgtcctggtt
     3841 atcgacgaga aactcacggg gcaaaacgtg caagatttgt cggtcggcgt ccatcgccac
     3901 ggcacgagcg ccttcgagca cacggtccac gtcggctgcc gtgacctctc ctcccgcgac
     3961 cgccacgaca ccatcggagt tttgcgagcg aatatggctt ccaccaacgc ccgcatacac
     4021 ggttcgaatt tccacggcgg ccatggattg cgccatttgc accgcctcgg tgatggattt
     4081 catggtccac tctatgttgg ccaccacccc ttttcgcagg cctttgcagg gcacgttgcc
     4141 cacgcccatg atggtgattc catcgcggtc cacttcaccc acgacggctg ctaccttcgt
     4201 ggtaccaata tccaagccaa caacgatctc ggagctttcc atcgattcct cacccaagtg
     4261 gcgaaccgct gacgtgcagc caacgaccat caccggaacg cctcggacgc tgccatgggg
     4321 cggttttcgg ggccaagttt tcgcaaacac aaaccgactt ttcgtccttc aaagtgcgaa
     4381 gtccggatcc ggatgatgat cggatcatca atatctgccg agatttgtcg ttcatcgcat
     4441 cctcaccacc acccgttcgg gatgcgcttc gttgtccaaa aacacgatgg ccggatcgcc
     4501 tttttgcttt ttttgaacct cttgaatgac ccgcgccgcc tgcaacacct tccgtggata
     4561 cggagaacgc cccatctcca gcgttatcgc gcttcgtcct accaccatgc gcaacgagcc
     4621 atcatccgca atcccgatct cctgaatggg atgctgctgc acaggccctc gcgacgcata
     4681 ctgcgccatc agttccaacg ccgaacgcac ccggctcacc gcaagcttcc gatcgttggc
     4741 caccaacgaa gatgtcagac cggtaatgac gatcaagccg atcggatcat cggcttccaa
     4801 ctttttgaat agctcccctt ccggcgtgca gaggtacaag tccgcatcga ccgcaacgac
     4861 cgccgcggct tctcgctccg tcaacgtaat cgtcaacgtg gcgggaagtt gtcgctctac
     4921 ctgcgccgtg gcaacccacg gatccaacaa caaccgattg cgcgccgcct cgaggtccac
     4981 cgagaaaaca ttcattccga cacgcaaacc agcagcctcg gatatttgct cgggggaacg
     5041 ccgcactgcc ccatcgatcc gaatggtctt caccgcaaaa cgcggactcg acaccacatg
     5101 ctgcttgatt ccccaagcca gcgcgatgga cgaggccacc acgaacacga tgcttgccgc
     5161 cgctcgcaag ccggaaagcc tggcccgaaa ccctcccgcc cacgatcgca accgtcctgc
     5221 cctgggcttt ttcgtggacc ccttcccctc gtttcccgcg tttcgagatg atgcggccga
     5281 agcctgtccc tcggcaggcg caacacgccg gttggatggc aacacgctgg gacggctcgg
     5341 ctcctccgtc gtcgatgacc tttttcggcc tatcatgccc actccttact catccctcgt
     5401 cggccccatc ctcggcttcc agacgacgcc ccaattcgcg aatgctttgg ttgatatccc
     5461 ccgctccctg cgcaatcacg acgtcgcccc gaagaaccaa tttctgtaag aaatccggta
     5521 tgttatcctt gttctggaca tagtgtacgc gttggtggcc gtgctcacga agagccttcg
     5581 ccaacagttg ccccgatacc cccgcaatgg gagtctcgcc cgcggggtaa atctccgtga
     5641 ccaccaccac gtccgccagg ttgaacgacg tgacgaattg ttcgaaaaga tcgcgcgttc
     5701 gcgtgtatcg gtgaggctga aagacaacga ccaatcgtcg gtcatagcca gcccgcgccg
     5761 cttccaacgt cgcccgaatc tccgcgggat ggtgcccgta atcgtcgatg agcgtgacgc
     5821 ctttggcctc ccccaccacg gtgaaacgtc gcgcaactcc ttcgaaatcc gccaacgatt
     5881 gctgaatcac atcgagcggc acggcaagtt catcggccac ggcaatggtg gccaaacagt
     5941 tcaagacatt gtgtcgccca ggcatacgca ccgaataatt tcccagcagt tccccctgct
     6001 tgagcacctt gaagcgcatt tccgttccga cggccttcac gtccttcgcc cgataatcag
     6061 cctgcgggga aaaaccgtag gtcgtatgcc gtcgtcgcac cgccggcagg atgctctgca
     6121 catcgggatg gtccaggcac aagatggcaa gcccataaaa cggcacgcgg gaacagaatt
     6181 ggatgaacgc ttctttgatc cgttcgtgac tgccccaata gtccaagtgc tccgggtcga
     6241 tgttggtcac cacggcgatg gtgggcgaaa gaagcaaaaa cgagccgtcc gattcatcgg
     6301 cttccgccac catcaagtcg ccagcaccga gtttggcgtt ggagcccaac gccgccattt
     6361 ttccacccac cgccacggtg ggatcgaacc ccgcggcgcg caacaccgtg gctaccaacg
     6421 aggtagtcgt ggtttttcca tgagaaccag caaccgccac accgtatttg acgcgcatca
     6481 gttccgcgag cagctcggcc cgggcaatca ccggaatgcc gctggtaagc gcttcgacca
     6541 actccggatt gtccggcttg atcgcactcg aatacaccac cacatcgctg cccacgatat
     6601 tggcggcccg atgcccgatg tggatcttca ccccaagggc gtcgagccgt tgcgtggtgc
     6661 tgtttggctt gagatccgag ccgctcacct caaacccgag ggcatgaacg atttcggcaa
     6721 gaccgctcat gccgattccg ccaatcccga cgaagtgaat ctgtcgaaca cgaccacgaa
     6781 acatcaaaga acctccgcga gatggagcaa atcgtccgca atggccgccg ccgcttcgga
     6841 acgtccatgc gctctggccc ggtcggccat ccgccttcga gccttttcat cgttggccag
     6901 acgcaccagg tggtcggcga gcacggtggg cgaagcatcg ctcgagggga tgcaaatcgc
     6961 cgcaccgtcg ctttccagcg cccgggcgtt atgaagttgg tggtccccc
//
LOCUS       DAOSOR010000298         6991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10837_length_6991_cov_4.645905, whole genome shotgun sequence.
ACCESSION   DAOSOR010000298 DAOSOR010000000
VERSION     DAOSOR010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10837_length_6991_cov_4.645905"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /locus_tag="PK710_15290"
     CDS             complement(<1..1294)
                     /locus_tag="PK710_15290"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOT11143.1"
                     /translation="MKRFHRLTTIAFLTIASVGFGCVGGSKSSQANPEALKQYILEAP
                     PSDIPHKLNAVFEGKAKLIGYRVSPEKEAKPGSEIKLTMYWECLDNIESGWNLFTHVF
                     DANDNRILNIDNVGPLRQWVDNKQVLAPSSWKKGKVYVDEQVFSLPDKLDTHEIVITT
                     GIWKEEARLKITSGPHDRENRAIVARLSTGISRKPKPFTGIKSLRVDKLAKGDTITID
                     GKLDEEAWKKAASTGPFVDVGTGEPNASFPVQGSARLTWDDSFLYVAFEVQSATIVGG
                     FPNDAKDPHLWEKDTVEIMIDPDGDGDNKDYYEIQINPQNLVFDTQYDSYNQPKDDSK
                     GMFGNMDWSAKLKSAVVVEGEMDAPDKGKGYVVEAKIPWASFSKANQSPPKPGNTWRM
                     NFYAMKNNSGVAWSPILGQGNFHRAARFGRIVWAVPGEP"
     gene            complement(1364..2983)
                     /gene="ffh"
                     /locus_tag="PK710_15295"
     CDS             complement(1364..2983)
                     /gene="ffh"
                     /locus_tag="PK710_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOT11144.1"
                     /translation="MFEALTKGFRQARNRLAGLTELTEANIEPALREVRLSLLEADVD
                     LSVVKRFLAHIKQQALGQTLRTKVKVDGVTREVSAGDQFIKLCHDELVRMMSSDSEPI
                     TWAENGPTGIMMVGLQGSGKTTTAAKLARLLERDGKKPLLVAADMQRPAAVDQLQVLG
                     KQIGIPVFNIKQTPVEICTAAIDFAKRHKHDVIIYDTAGRLAIDEVLMNELDDIKANV
                     WPENILLVVDAMIGQDAVKTAKAFHEKLQITGVVLTKLDGDARGGSALSIKEVTGAPV
                     VFTGVGEGTSQFEPFRADGMASRILGFGDVVGLMQDFEDAIDKKQATEKALKMLEGEF
                     TFDDFLDQVRTIQKMGSLRDLVDKMPFFPQGLPEGVNLDDKELVRIEAIIQSMTFAEK
                     RDPYVLIREPSRVKRIAKGSGQPEIGVQELVQKFLFMRQMMTGMKQNMGLMGKIPGLK
                     QIASARQMRKAMAGGAMPPGFPGFDGMGMPGFDGMFGGLGGMPSMPGFGLPQPQFDSP
                     TKMKPLSKSEKNQRKAQRRRERDARKKSRQK"
     gene            complement(3105..3530)
                     /locus_tag="PK710_15300"
     CDS             complement(3105..3530)
                     /locus_tag="PK710_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11145.1"
                     /translation="MQNRSQFAIGCLAITIGLGVVSAASACKHSTDKTEATDILRVIN
                     NLRMADNDQKRAPLEHLKSLPCSTTETCQAQKNCIVAFEHHVRGTELGQRLKARLQQQ
                     PTDDQAAMLLEMNIEIEEGKRAMPACEQQVTTLRKRYKI"
     gene            3588..5870
                     /locus_tag="PK710_15305"
     CDS             3588..5870
                     /locus_tag="PK710_15305"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOT11146.1"
                     /translation="MTSIRPPSSSLREDALRYHSQGRAGKIEIALTKPCSSQRDLSLA
                     YSPGVAEPCLEIAKDPNKAFDYTARGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGV
                     LFKRFADIDVFDIELNLKDPEKIIECVAALEPTFGGINLEDIKAPECFEIEKRLKERM
                     QIPVFHDDQHGTAIISAAGLINALELQGKKIEDVRVVVSGAGASAIACCNLWVKLGIR
                     RENITMCDSRGVLHEGRRGTVDVTKEAYIVDTKARTVEEALRGADVFLGLSKGNLLTP
                     DTLRTMAPKAIIFALANPDPEVPYNVAKEVLPDAIVATGRSDYPNQVNNVLGFPFIFR
                     GALDVRARAITEEMKLAASNALARLAREDVPESVQAAYGNTKIEFGPDYIIPKPLDER
                     VLLWEAPAVAQAAMDCGVARIKVDIDEYRASLERRLGPSRSFIRQFMGKVKKTNKTIA
                     FPESDDPRILRACRVLVEEDIAKPVLVGDTEAIQLRAAHEGVSLRGMEIVDLEKQDRI
                     DAAVADLLEARGRRGLTEPRARQLALRDPTTVAMLMVRRGEVDGAIAGIHHGYPETIR
                     MALKLVGLAEGVNTPTGVHILVGNKHGPLFFADTSVNIAPDAPLLADIAIVTARFVRE
                     LGIEPKVAMISFSNMGGSPHRYARKVNEAARLVQQREPTLMCEGELQAQVALDEELLR
                     RYWPHAKLQGMPNVFVLQSLGAANSVYQFLSRLGGFDEIGPVLLGLRRPVTVISPRAP
                     VNRIVQMAALTALHSIKGTF"
     gene            complement(5924..6721)
                     /locus_tag="PK710_15310"
     CDS             complement(5924..6721)
                     /locus_tag="PK710_15310"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT11147.1"
                     /translation="MQRIRFTTTGWMETPGFMLGLPHRHNVPIPAVVAICEKSNGTIV
                     LVDAGWSAEECRRPSSIGLAHQHILGVRVQPQQDVASQMRRAGLDPHRVTTIIATHLH
                     LDHIGGAVDFPNAEIIAAHNEVTEAYRSSWMKGYRRKDLECLRRLRPVRLSPMPLLGF
                     SHSFELDEEITLLDTHGHTSGHLAVAIRQGSTLWIHGGDVAYQHRELSSGSLCPLSQF
                     LAHDRACARVAQRRLYRCGQEHPHVRVVLSHDAQGFATLPALDEQMP"
     gene            complement(6810..>6991)
                     /locus_tag="PK710_15315"
     CDS             complement(6810..>6991)
                     /locus_tag="PK710_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11148.1"
                     /translation="IERRLSTRFLAPFNCPKERIMFLMLSKKSRRTNRNRTARFKAKL
                     AAKNRRRRAKIYQLT"
BASE COUNT         1330 a         1661 c         2274 g         1726 t
ORIGIN      
        1 ggggttctcc tggcacggcc cagacaattc gtccgaagcg agcggctcga tggaagttgc
       61 cttgaccgag gatgggggac caggcgaccc ctgaattatt tttcatggcg tagaagttca
      121 ttcgccacgt atttccaggt tttggggggg attggttggc tttcgagaag gaggcccatg
      181 ggattttggc ttccaccacg tagccttttc ctttgtcggg ggcatccatt tcgccttcga
      241 cgacgacagc ggatttgagt tttgcggacc agtccatatt gccgaacatg cctttcgagt
      301 cgtctttggg ctggttgtac gaatcgtatt gtgtatcgaa gacgaggttt tggggattga
      361 tttggatttc gtagtagtcc ttgttatcgc catcgccgtc gggatcgatc atgatttcga
      421 cggtgtcttt ttcccatagg tgggggtctt tggcgtcgtt ggggaatccg ccgacgatgg
      481 ttgcgctttg tacttcgaaa gcgacgtaaa gaaaggagtc atcccaggtg agacgagcgg
      541 agccttggac gggaaacgac gcattgggtt cgccggttcc cacatcgaca aaagggccgg
      601 tggatgccgc tttcttccag gcttcttcgt cgagttttcc atcgatggtg atggtgtcgc
      661 ctttggcgag tttgtcgacg cgcagggatt tgattccggt gaaaggtttg ggtttgcggc
      721 tgattccggt ggagagtctg gcgacgatgg cgcggttttc gcggtcgtgg ggtccggagg
      781 taattttcag gcgtgcttct tctttccaga tgccggtggt gatgacgatt tcgtgggtat
      841 cgagtttgtc tgggagcgag aagacctgct catcgacata gactttgcct tttttccagg
      901 aggatggggc gaggacttgt ttgttgtcga cccattggcg cagggggccg acgttatcga
      961 tattgaggat gcggttatcg ttggcgtcga agacgtgggt gaagaggttc cacccacttt
     1021 cgatattgtc gaggcattcc caatacatgg tgagtttgat ttcggaaccg ggtttggctt
     1081 ctttttcagg ggagacgcgg tatccgatga gtttcgcttt tccttcgaag acggcgttga
     1141 gtttgtgggg aatgtcggag gggggtgctt cgaggatata ttgtttgagg gcttcggggt
     1201 ttgcttggct ggatttggag ccgccaacgc aaccgaatcc gacggatgcg atcgtgagga
     1261 acgcgatagt cgttaatcga tgaaatcgct tcatggtact cgcctgcctt gcggtgcccc
     1321 ctttggggcg tgaaaggttc ggctgcatga gccgagcggg tttttatttt tgtcgggatt
     1381 tttttcgtgc gtcccgctct cgtcgtcgct gcgctttgcg ctgatttttt tcggacttgg
     1441 aaagaggttt cattttggtc gggctatcga attggggttg aggcaagccg aagcccggca
     1501 tggatggcat accgcccagt ccaccgaaca tgccatcgaa tccgggcata cccatgccat
     1561 cgaatcccgg gaatccggga ggcatggcac cgccggccat ggcttttcgc atctgtcggg
     1621 cggaagcaat ttgtttcagg cctggaattt tccccatcaa gcccatgttc tgtttcatgc
     1681 cggtcatcat ttgtcgcatg aacaggaatt tttgaacgag ttcttgcacg ccgatttcgg
     1741 gctgtccgga ccctttggcg atgcgtttga cgcgggaagg ttcccgaatg agcacgtacg
     1801 gatcgcgttt ttccgcaaag gtcatggact ggatgatggc ttcgatgcgt acgagttcct
     1861 tgtcatcgag gttgacgcct tcggggagcc cttggggaaa gaagggcatt ttgtcgacga
     1921 ggtcgcgaag ggagcccatc ttttggatgg ttcgaacttg gtcgaggaag tcgtcgaagg
     1981 tgaactcccc ttcgagcatt ttgagagctt tttcggtagc ttgcttttta tcgatggcgt
     2041 cttcgaagtc ttgcatgagt ccgacgacat caccgaagcc aaggattcgg ctagccatgc
     2101 cgtcggcgcg gaaaggttcg aattgactgg tgccttctcc gacgccggtg aacacgacgg
     2161 gagcgccggt gacttctttg atggaaagcg cggagccgcc tctggcatcg ccgtcgagtt
     2221 tggtaaggac aacgccggtg atttgcagtt tttcgtggaa agccttggct gtttttacgg
     2281 catcctgacc gatcatggcg tcgaccacga gcaggatatt ttcgggccaa acgttggctt
     2341 tgatgtcatc gagttcgttc atcagaactt cgtcgatggc gaggcgtccc gcggtgtcat
     2401 agatgatgac gtcgtgtttg tgtcgtttgg cgaagtcgat ggcggcggtg cagatctcga
     2461 cgggggtctg cttgatgttg aacacgggga taccgatttg ttttccgagg acttggagtt
     2521 gatccacggc ggcgggtcgc tgcatgtctg cggccacgag aagcggtttt ttcccgtcgc
     2581 gttcgagcag acgagcgagt tttgcggcgg tggtggtttt gccggagcct tgtaggccca
     2641 ccatcatgat gccggtgggt ccgttttccg cccaagtaat gggctcggag tccgaggaca
     2701 tcatgcggac gagttcgtcg tggcatagtt tgatgaactg gtcaccggcg gatacttcgc
     2761 gtgttacgcc atcgactttg actttggtgc gcagggtttg gccgagggcc tgttgtttga
     2821 tatgggcgag gaaccgtttg accacgctaa ggtcgacatc ggcctcgaga aggctgagtc
     2881 ggacttcacg cagagcgggt tcgatgttgg cttctgtgag ttcggtcaag ccggccaaac
     2941 ggttccgagc ttgacgaaag cctttcgtga gtgcttcaaa catggcttgt cctggagtct
     3001 ggcgcttgta gcacgtcgcg acggggtggg ggaggggtgt gggaggttgt tcggaacctc
     3061 gggaggaaga ttcctggcgg catgatcgca actaattgaa attattagat tttataacgc
     3121 ttccggaggg tggtgacctg ctgttcacaa gcgggcatgg cgcgttttcc ctcttcgatt
     3181 tcgatgttca tttcaagcag catcgcggct tggtcgtcgg taggttgctg ctggagacga
     3241 gctttgagtc gttgacccaa ctcggtgcca cggacgtggt gttcgaaagc gacgatacag
     3301 tttttttggg cctggcatgt ttcggtggtg gaacagggaa gggatttgag gtgttcgagg
     3361 ggagcgcgtt tctggtcgtt gtcggccatg cgcaagttgt tgatgacgcg aaggatatcg
     3421 gtggcttcgg ttttgtccgt cgagtgtttg caggcggaag cggccgagac gactcccagg
     3481 ccaatcgtga tggcgagaca accgatggcg aattgcgagc gattttgcac gaagacctca
     3541 ttatacagtg gcttgcgtcg cgtaaccaac cttgctaacc gacggccatg acaagtattc
     3601 gacccccttc gagcagcctt cgggaagacg cgcttcggta ccattcccag ggcagagcag
     3661 gaaagattga gattgcactg accaagccgt gctcatcgca gcgcgacttg tcgttggcct
     3721 acagtccagg tgttgccgaa ccttgtttgg agatagccaa agatccgaac aaggcatttg
     3781 attacaccgc gcgcggcaat cttgtggcgg tgatttcgaa cggcacggcg gtgcttggtc
     3841 ttggcgacat tggcgcattg gcgggcaagc cggtgatgga aggcaaaggc gttttgttca
     3901 agcgatttgc cgacatcgac gtattcgata ttgaactcaa tctcaaggat cccgaaaaaa
     3961 tcatcgagtg tgttgcggcg ttggagccga ccttcggtgg gatcaatctc gaggacatca
     4021 aggcaccgga gtgtttcgaa atcgaaaagc gtctcaagga gcggatgcag attccggtgt
     4081 ttcatgatga tcagcatggc acggcgatta tcagtgccgc ggggctcatc aatgccctcg
     4141 agctgcaggg aaagaaaatc gaagatgttc gggtggttgt gtcaggcgcg ggcgcttccg
     4201 ccatcgcgtg ttgcaacctt tgggtcaagc tcggcattcg gcgagagaac atcaccatgt
     4261 gcgactcgcg gggtgtgctt cacgaagggc gtcgtgggac agtggacgtg acgaaagaag
     4321 cctatatcgt ggacacgaag gcgaggaccg tggaggaagc gcttcgcgga gcggacgtat
     4381 ttcttggtct ttcgaagggg aatttgctta cgccggacac gcttcggacg atggccccca
     4441 aagcgatcat ttttgctttg gccaatccgg atccggaggt tccgtacaac gtggcgaagg
     4501 aagtattgcc ggatgcgatt gttgcgacag ggcggagcga ctatccgaat caggtcaaca
     4561 acgttcttgg atttcccttt attttccgcg gggctctcga cgttcgggcg cgagcgatca
     4621 ccgaggagat gaagctggcg gcttctaacg cattggcgcg tcttgcgcgg gaagacgtgc
     4681 cggaatccgt gcaagcggct tatgggaaca cgaagatcga gttcgggccc gactacatca
     4741 tccccaagcc tctggacgag cgtgtgttgc tatgggaggc gccggccgtg gcgcaggccg
     4801 ccatggattg tggggtcgca cgcatcaagg tcgatattga cgaataccgt gcatcgctcg
     4861 aacgtcggct tggaccgtcg cgttctttca ttcgccaatt catggggaag gtgaagaaaa
     4921 ccaacaaaac gatcgcgttt cccgaaagcg acgatccgcg gattttgcga gcgtgccggg
     4981 tgttggtgga ggaggatatt gccaagccgg tgctcgtggg ggatacggaa gccattcaat
     5041 tgcgtgcggc gcatgaaggg gtatcgcttc gcggcatgga gatcgtggac ctggagaagc
     5101 aggaccggat cgatgcggca gtggcggatt tgctcgaagc ccgcggccgt cgtgggctta
     5161 cggaaccgcg agcccgacag cttgcgctgc gcgatcccac tacggttgcc atgctgatgg
     5221 ttcgtcgtgg tgaggtggat ggtgcgattg cgggaatcca tcacgggtat cccgagacga
     5281 tccgaatggc cctcaaactc gtgggtcttg cggagggagt gaacacgccc acgggtgttc
     5341 acattctggt gggcaacaag catggtccgc tcttttttgc ggacacgagc gtgaacatcg
     5401 cgcccgatgc tccgttgctg gcggatattg cgatcgttac cgcacgattc gttcgcgagc
     5461 tgggtatcga gccgaaggta gcgatgattt cgttttcgaa tatgggaggt tcaccccatc
     5521 ggtatgcgcg taaggtcaat gaggcggcgc gtctcgttca gcagcgcgaa cccacgctga
     5581 tgtgtgaggg cgagcttcag gcgcaagtgg cgttggatga agagcttctg cggcgctatt
     5641 ggcctcatgc caagctccag gggatgccga acgtattcgt gcttcagagt cttggcgccg
     5701 ccaatagcgt ctaccagttc ctcagtcggc taggtggatt tgatgaaatc ggtccggtac
     5761 tgcttggcct caggcgtccg gtgacggtca tttcgccgcg cgcgccggtc aatcgcatcg
     5821 tgcaaatggc cgcgttgacc gcgctgcatt ctatcaaagg aacattttga tcagggttga
     5881 ggagcggtga accattctcg gtgcgccgct ctcgtccctc atttcatggc atttgctcat
     5941 cgagcgcagg gagcgtcgca aagccctggg catcgtgcga gagcaccaca cgaacgtgtg
     6001 gatgctcttg gccgcatcga tacagtcttc gttgtgccac tcgtgcgcat gcccggtcgt
     6061 gcgccaagaa ttgagacaag gggcatagcg atccactgct aagctcccgg tgttgatagg
     6121 ccacgtcgcc accatggatc cacaatgtcg agccttgtcg gatagcaacc gctagatgac
     6181 cggaagtgtg cccatgtgta tccaacaacg tgatttcttc atccagctcg aaggagtgag
     6241 aaaagccgag gaggggcatt ggacttaggc gtacgggccg aagacgtcga aggcattcga
     6301 ggtccttgcg tcgatatcct ttcatccacg acgagcgata ggcttcggtg acttcgttgt
     6361 gggcggcgat gatttccgca ttggggaagt cgacggcccc tccgatatgg tcgaggtgca
     6421 agtgggtagc gatgatggtg gtgacgcgat gggggtcgag tccggcgcgt ctcatttgag
     6481 aggcaacgtc ttgttggggc tgcacacgca cgccgagaat gtgttggtga gccaggccga
     6541 tggacgaggg gcgtcggcat tcttcggcgc tccatccggc atccacgagt acaatcgtgc
     6601 cattgctttt ttcacatata gcgacgacgg cggggatggg gacgttgtgt cgatggggga
     6661 gtccgagcat gaagcctggt gtttccatcc atccggtagt tgtgaaacgg atccgctgca
     6721 cggtagtcct catccgtggg tgttagcagg cattatgagg ccaagaaggg aaacgatagg
     6781 ccaaaggacg tcttgtgtat ggtgtctttt caggtgagct gatagatttt agcacggcgg
     6841 cgccgatttt tggcggcgag tttggccttg aatcgagcgg tgcggttacg gttggtgcga
     6901 cgtgattttt tcgaaagcat gaggaacatg atgcgctcct tagggcaatt gaaaggagca
     6961 agaaacctag tcgacaggcg tcgctcgatc a
//
LOCUS       DAOSOR010000299         6948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_10943_length_6948_cov_4.687654, whole genome shotgun sequence.
ACCESSION   DAOSOR010000299 DAOSOR010000000
VERSION     DAOSOR010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6948
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10943_length_6948_cov_4.687654"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PK710_15320"
     CDS             complement(<1..600)
                     /locus_tag="PK710_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11149.1"
                     /translation="MRQVRLLAGRYRVLGRLSANPRRAVWLAEEAASSRPMLVAQINQ
                     AKAQWMSPLVGVTHPHLAPIIRIVAPVVRAELPLAEDETAPEAVAIAELIAGRTLTQA
                     IAHGPLPVHQSVRCTAYVAAAVDALHRRGAAHGSISDRSMVIERADEGRSPVLTQLSE
                     PPFAAFASPERLRGEGPSQQDDVWALHVMLYLALVGSLPF"
     gene            complement(738..1763)
                     /locus_tag="PK710_15325"
     CDS             complement(738..1763)
                     /locus_tag="PK710_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11150.1"
                     /translation="MRQKMNFSDRLQEAVGGYPPLFDRDISVDNVDPRRSCLQPVYQR
                     FRDASHRLALPPDRGEWLDFESGRKPMLRLVLDSAEYRRLLQHSRDFGWVTALVDRSS
                     SHEKSSVDPSRKTILLGREIQTVKEAARLDSELLDGRVTIDEWQTLGRRFGELLGYPS
                     CCVEHFISLPPNASNEQVIRSIAVRSKRFEPWLNNLTMSAYHAICWFPCRYDCDASLR
                     IARHVDGLLRTSCSSNHYHRVQHFLSMPRLVFDERRQLLFGGAVQHHGRVHYHSVYSL
                     FSFDFQPSFVWTDWLFFIDVVMHFQQADWFEHHDPLMVIGKKDATTVEFRCHRSPTWM
                     PFGWQDG"
     gene            complement(1748..2158)
                     /locus_tag="PK710_15330"
     CDS             complement(1748..2158)
                     /locus_tag="PK710_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11151.1"
                     /translation="MPNPKSSTKDIIINVLILAVAGGVLAWRYNDCKQKEKQAEQAAQ
                     QARAHEEKERTQRTNACLKLVPETQQNDCTQCTCTKCVDVVEACEADKQCRTMSVDTL
                     LKDGGPPSDDPSRIRYENRAKCMLTECHDACAKK"
     gene            complement(2281..2829)
                     /locus_tag="PK710_15335"
     CDS             complement(2281..2829)
                     /locus_tag="PK710_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11152.1"
                     /translation="MMNTTYLTSRSRRTAGWACRIAGGLLAFTLVLTAVSCAKKPTLQ
                     LHHAQISHVSPMGVVANVVVKVNNSNSFDIQVRNMYAQTTLAGHFQLPPINIQPNIWL
                     PAKETTYVTTPVTIPWTMIPGVMMTTLGNEYISYQVQGYADVTATRSLGVKVNNEPLN
                     EQGLIPREMMLQAARPTLPNLR"
     gene            3023..4762
                     /locus_tag="PK710_15340"
     CDS             3023..4762
                     /locus_tag="PK710_15340"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HOT11153.1"
                     /translation="MLKRSFLFVSLISLIAACSSTVDTTESSDKGEPVAPPADGKMDV
                     AERIIDKGELGFGPENAVSAAFDEDLQFYGWHLHVRAGASVTLEVTQKGSSRNLDTTL
                     YVYGPKTSSGGYGTTALGFDDDTGWGRLSRITEHSFHVAGEYLVVVGTYDGRGRGNFR
                     LQATCLSGDCSPEAKPEPVVTDCHADIANAILACVEAKENSSTEDVSTMTRQDLIEAC
                     TTPEQVASAWEKLCAQSSAPSDVCASFEEFSSSYLPACGRELIDKTLDTTCVFGTTYP
                     DIWNRPGAVVVLEQRVLSQSDVLSPLEQQQVIAAVKSSAHSEVSSIDEAFAAADGGIV
                     YQTQLWDASHRKPYTSVELGAGDNSFGLIFDFGTVQPVATINDSDILECKVFWGSEMR
                     PCKQDEHCAGELSCTGVAEELEKGRCIDTSKDEHPVNGTECSDQEPCPEGTGFVCAGG
                     FMCNPAWMRGRFVSRPNLPIPDANPNGADATIVAYGLTTVSTDVALDLHITHERPSDL
                     VVSVINPEGTEVVVFDKKQGVNEIFFDSLPVEGFPGDESANGVWTVRAVDTKTGSTGI
                     IRSFSLTITSRWD"
     gene            4901..>6948
                     /locus_tag="PK710_15345"
     CDS             4901..>6948
                     /locus_tag="PK710_15345"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOT11154.1"
                     /translation="MSSYDHIGMTLDESLTGWIGIGQSDPVQGRVHGEAQGTRLTMHA
                     RLVVQQLRAFLEQPDHAAQLEGTIDLSSVGQAMVMQDGVFNVFVVNPRTGARQMVYEA
                     RFSDSSGKRYFLRGVKHLSDDPGFDVLEDMTTLFTMIYEGDDDQGAVFGAGQVYFPLS
                     TMPGFLRSLHVTGNPHFGQALAAKIAFLSFAFGQVRDIYLADINPVYDASYQNVVLQG
                     EVFDGTGREPFFLVSGIHTPDFPWGDGETFCDVLLMIGDPDHAPQFFAVSARRLKQTV
                     DVRGGILTYDGPIYDLTTHPVVSFSELQAATGLVAGQANIQLQFHAKPHPLAPFPFRI
                     HSEALDRLSYRIRETLRRMLPSEAQFGFEIVPHTVSSVTGTMEITVGATRRWQVDTAT
                     AFGEAEDSTVRNVREPTLLYGYICALRPGRTARVQFHTSTLRNEREYWGKDRLDAFVG
                     ALISRFASKDLDIRPDGITITDLSETNASRLFQRVGEPLLDIRNDQFPTAVFQRRLIR
                     VQDPSGDSCLALEEAMDTLRREPVDRTDRVTVASIRDSDPVAALTRALQDSGFWTEIE
                     KVRVAGGKEKSKFSVVIKPNFMFAYNKHDRSTYTDPALVEELVRQLRAAGYSRISVVE
                     AQSTYGQYFAHRSVREVASYLGYAIDGSRGYEVVDLTTDTQQFQHFGPALGYHPVANT
                     "
BASE COUNT         1504 a         1907 c         1959 g         1578 t
ORIGIN      
        1 aaatggcaga ctgcccacca acgcgagata aagcatgaca tgaagcgccc acacgtcgtc
       61 ttgttgactc ggcccttcac cacgcaatcg ctcgggtgac gcaaaggcag cgaaaggagg
      121 ttcgctgagt tgtgtaagaa cgggacttcg tccttcgtcc gctctttcga taaccatact
      181 cctgtccgaa atcgacccgt gcgccgcgcc gcgtcgatgt aatgcatcga cagcagccgc
      241 cacataggcg gtacaacgaa cactttgatg aacgggcaga gggccatggg caatggcctg
      301 ggtcaaggtg cgtccggcaa tcagttccgc gatggccacg gcctccggtg cggtttcgtc
      361 ttcggcaaga gggagttccg cgcgaaccac aggggcaacg attcggatga tcggggctag
      421 atgtgggtgg gtaacaccaa cgagtggact catccactgc gctttggctt ggttgatttg
      481 agccacgagc atagggcgac tggatgcagc ctcttcggca agccaaaccg cacgccgtgg
      541 gttggccgat agacgtccca acacacggta tcgtcctgca agaagtcgga cctgtcgcat
      601 tgtcttcacg agaaatacgc ttccctccag ccgggtccgt aagcatatcc gtgaggatat
      661 gggccatcac aaaggctcaa tacccatagc ctcagcggca ggatcgacgc aaggttcgga
      721 tatctcgaag cgtgggtcta cccatcttgc catccaaagg gcatccaggt cggagaccgg
      781 tggcagcgaa actcaaccgt cgtagcatct tttttgccaa tcaccatcag tggatcatga
      841 tgctcgaacc aatcggcttg ctgaaaatgc atcaccacat cgatgaaaaa tagccaatcc
      901 gtccacacga aagacggttg gaagtcgaac gaaaacagcg aatataccga atggtaatgc
      961 acgcgcccat ggtgctggac cgctcctccg aagagcaatt gccgacgttc atcgaacacc
     1021 aatctgggca tggacaggaa atgctggacg cgatggtaat ggtttgaact acaggaggtt
     1081 cgtagcaatc cgtcgacatg tcgagcaatg cgcagcgatg cgtcgcagtc ataacgacaa
     1141 gggaaccaac agatcgcgtg ataggccgac atggtgaggt tattgagcca tggttcgaac
     1201 cgtttcgatc gaacagcgat gctgcgaatc acctgctcgt tggacgcgtt cggtggaagc
     1261 gaaatgaagt gctcgacaca gcacgacggg tagcccagca gctctccgaa ccggcgccca
     1321 agcgtttgcc attcgtcgat ggtcactctg ccatcaagca gttcgctatc gagtcgcgcc
     1381 gcctccttga cagtttggat ctctcttccc agcagaatgg tcttcctcga tggatccacc
     1441 gaacttttct cgtggctgct cgaacggtcc accaaagcag ttacccatcc gaagtcacgg
     1501 ctatgctgca gcaatcgacg gtactcggcg gagtcgagaa caagacgaag catgggtttt
     1561 ctgccgctct cgaagtccag ccattctccg cgatcgggag gtagggcgag acgatgggat
     1621 gcatcgcgaa agcgctggta gacaggctgc aaacacgaac gccgtggatc cacgttgtcg
     1681 acggatatgt cgcggtcgaa tagcggtggg taacctccga cggcctcctg taacctatcg
     1741 gaaaaattca ttttttggcg catgcgtcgt ggcattcggt cagcatgcac ttggcgcggt
     1801 tttcgtaccg gatgcgcgat ggatcgtcgg acgggggacc gccatctttg agtaacgtgt
     1861 cgacggacat ggttctacat tgcttgtccg cctcgcatgc ttcgaccaca tcgacgcact
     1921 tggtgcatgt gcattgtgtg cagtcatttt gctgtgtctc gggcacgagt ttcaggcatg
     1981 cgttcgtgcg ttgcgttcgt tctttttctt cgtgggcgcg ggcttgctgg gccgcttgct
     2041 cagcctgctt ttctttttgt ttgcagtcgt tgtagcgcca agcaagcact ccacccgcga
     2101 cggccaagat gaggacgttg atgatgatgt ctttggtcga cgacttcggg ttcggcatga
     2161 gcggtcattc tcccaggggc ctttggtggt aatgatcaac acatcttgta ggaagggacc
     2221 atagacgatt gcactcgatg tggcgtcggg cttcggaaaa aaagattgcg gaagatgctg
     2281 ttagcgaaga ttgggaagcg tgggtcgagc agcttgaagc atcatttctc tgggaatcag
     2341 cccttgttcg ttcaacggct cattgttgac cttcacccca agtgaccgcg tggcggtgac
     2401 atcagcgtat ccttgaacct ggtaactgat gtattcgttt cccaacgtgg tcatcattac
     2461 ccccggaatc attgtccatg gaatcgtaac cggtgtcgta acgtaggtgg tttccttggc
     2521 gggaagccag atattcggtt gaatgttgat cgggggaagt tgaaagtgcc ctgccaacgt
     2581 cgtttgggcg tacatgttcc gaacttgaat gtcgaaactg ttgctgttgt taaccttgac
     2641 caccacgttc gccacgaccc ccatcggact cacgtgggaa atttgcgcat ggtgcaactg
     2701 caaggtgggt tttttggcac atgacaccgc ggtgaggaca agggtgaaag ccagcagccc
     2761 gccagcgatt cgacaggccc atccggcagt tcgacgtgat cgtgaagtaa ggtaggtcgt
     2821 gttcatcatg gatcccatgt ccacctagaa tgaaaagctc atgaatccta gcatgtatac
     2881 gcttgccggg acggtgttct tccgtgatcg ggtcgatatt ctgtctcgtt taacttccca
     2941 tcgaaaagtg gtcgaacggc ggttgctcat gagcgtttcg catgctagcc ggacggtctg
     3001 gatagcaagg agtagttttg acatgctcaa gcgttcgttt cttttcgtgt ctttgatttc
     3061 tttgatcgcc gcctgcagct ccacggtgga tacgacagaa tcttcagaca aaggcgagcc
     3121 tgttgctcct ccggccgatg gaaaaatgga tgtcgctgaa cgtatcatcg acaagggaga
     3181 actcggcttc ggacctgaaa acgcagtatc cgccgcgttc gacgaagacc ttcagttcta
     3241 tggctggcac cttcacgtcc gtgcgggggc tagcgtgacc ttggaggtca cacaaaaagg
     3301 gtctagccgc aacctcgaca cgacgcttta tgtgtacggt ccaaagacat cgtcgggtgg
     3361 ttatgggacc acagcgctgg gcttcgacga cgatacgggg tggggaaggc tgtcgcgtat
     3421 cacggagcac tcgtttcatg tcgccggcga gtatctcgtc gtggtgggga cgtatgatgg
     3481 ccgaggccgt ggaaacttcc ggctccaagc cacatgcctg tctggggact gctcgcccga
     3541 agccaaaccc gaacctgtcg ttaccgactg tcacgccgat attgccaatg cgatcctcgc
     3601 ttgcgtggag gcgaaagaaa acagttcgac agaagatgtc agcaccatga cgcgccaaga
     3661 cctcatcgag gcttgcacga ctcccgaaca agtggcgtcc gcatgggaaa aactatgtgc
     3721 gcaaagctcg gcaccctccg atgtatgcgc cagcttcgag gaattctctt cctcttatct
     3781 tccggcctgc ggtcgcgaac tcatcgacaa aacgctcgat accacatgcg tctttggaac
     3841 cacctatcca gatatctgga atcgtcccgg tgcggttgtc gttctcgaac agcgtgttct
     3901 ttcccaatcc gatgtcttgt cgcccttgga acaacaacaa gtcatcgccg ccgtcaaaag
     3961 ctccgctcat tcggaagtct catcgatcga tgaagctttc gccgcggccg atggcggtat
     4021 cgtttaccaa acccaattgt gggatgcgag ccatcgcaag ccctacacga gcgtagaatt
     4081 gggggcaggg gacaattcct tcggtctgat cttcgacttt ggaaccgtgc agcccgtggc
     4141 gaccatcaat gactccgata tcttggaatg caaggtgttc tggggttccg aaatgcgacc
     4201 ttgcaagcag gatgaacact gcgcgggcga gttgtcatgc acgggcgttg ccgaagaatt
     4261 ggaaaaaggg cgatgcatcg atacaagcaa agacgagcat cccgtcaatg ggacagagtg
     4321 ttccgaccaa gaaccatgcc ccgagggaac aggcttcgtt tgcgctggcg gtttcatgtg
     4381 caatcccgca tggatgcgag gacgtttcgt cagtcgtccc aaccttccca ttcccgatgc
     4441 caaccccaac ggcgcggatg caacgattgt tgcgtatggt ttgacgaccg tcagcaccga
     4501 tgtcgccctc gacctgcata ttacccatga gcgcccgagc gatctcgttg tctccgtcat
     4561 caatcccgaa ggaaccgagg tcgttgtatt cgacaaaaaa caaggtgtga acgagatctt
     4621 cttcgactct ctgcccgtgg aaggattccc gggcgacgaa tccgccaatg gcgtttggac
     4681 cgtccgcgcg gtcgacacga aaaccggatc taccggcatc atccgatcgt tttccctcac
     4741 catcacctcc cgctgggatt gattcccgtc gtcgatgcag ggcgattgat tatcgtccac
     4801 gcttgttgac ccgccatatc gtcttcgccg ttgaccacac tcgttcgtcc gtggacacta
     4861 cgcccgcgcg ggttacgcca acgggagcaa ggaggcggcg ttgagttctt acgatcacat
     4921 cgggatgacg ctggatgagt cgttgacagg gtggatcgga atcgggcaat ccgatcccgt
     4981 gcaaggacgc gtgcatgggg aagctcaagg cacgcgcctt acgatgcacg cacggctcgt
     5041 ggtccaacag cttcgagcgt tcctcgaaca acccgatcat gcagcacaac tcgaaggaac
     5101 catcgatctg tcgtccgtcg gccaggccat ggtcatgcaa gacggtgtgt tcaacgtgtt
     5161 cgtcgtgaat cccagaacgg gcgctcgaca aatggtgtac gaggcacggt tctccgattc
     5221 atcgggcaaa cgctatttcc ttcgtggagt caagcacctt agcgacgatc ccgggttcga
     5281 tgtccttgag gatatgacga cccttttcac catgatctac gagggcgacg atgatcaggg
     5341 ggcggtgttc ggtgctgggc aggtctattt tccgctatcg acgatgcccg gttttttgcg
     5401 ttctcttcac gtcaccggca atccccattt cggtcaggcc ctggcggcga aaatcgcgtt
     5461 tttatccttt gctttcgggc aagtacgcga catctacctt gccgatatca accccgtcta
     5521 cgacgcttct taccaaaatg tcgtgttgca aggggaagtg ttcgatggaa ccgggcgtga
     5581 gccattcttt ctcgtctcgg gcatccacac accggatttt ccttggggag acggggaaac
     5641 gttctgtgac gttctcttga tgatcggaga cccggatcac gcgcctcagt tttttgccgt
     5701 gagcgccaga aggctgaagc agaccgtgga tgtgaggggc ggaatcttga cctacgatgg
     5761 cccgatttat gacctgacca cgcatccggt ggtttctttt tccgaactgc aagcggccac
     5821 aggtctcgtg gcaggtcagg cgaacatcca attgcaattt cacgccaaac cccatccact
     5881 cgctcctttc cccttccgca ttcattccga ggccctcgat cggttgagtt atcgcatccg
     5941 ggagacgctt cgccgcatgc tgccgtccga agcacaattt ggcttcgaaa tcgtgccgca
     6001 cacggtctct tcggtaacgg gaaccatgga aataacggta ggtgcgacac gccgttggca
     6061 agtcgatacc gccaccgcct tcggagaagc cgaggattct accgtccgta acgtacgaga
     6121 acccacgtta ttgtacggtt atatttgcgc gttgcgtcct ggtcgaacgg ctcgcgtaca
     6181 attccatacg agcacgctgc gtaatgaacg tgaatactgg ggcaaagacc gtttggatgc
     6241 tttcgtgggc gcgctcatca gtcgttttgc ttccaaagat ctcgatattc gccccgatgg
     6301 aatcaccatc accgatctca gcgaaacgaa tgcatcgcgt cttttccagc gcgtggggga
     6361 acccctgctc gatattcgca acgaccaatt ccctaccgcg gtgtttcagc gacgactcat
     6421 tcgcgtacag gacccttccg gagactcctg ccttgcgttg gaagaagcca tggatacgct
     6481 ccgtcgggag cccgtggacc gcacggatcg agtgacggta gcttccattc gggactccga
     6541 tcctgtcgca gccttgaccc gtgctttgca agatagtgga ttttggacgg aaattgagaa
     6601 ggtacgggtg gctgggggca aggagaaaag caagttctcc gtggtcatca aaccgaattt
     6661 catgtttgcg tacaacaaac atgatcggtc gacctatacg gatccggcct tggtggagga
     6721 actcgtacgg cagcttcgcg ccgctggtta ttcgcggatt tcagtggtcg aagcccagag
     6781 cacctatggg caatacttcg cccatcgaag cgtaagggaa gtcgcgagct atttaggata
     6841 cgccattgat ggaagccgag ggtacgaggt ggtggacttg acgaccgata cgcagcaatt
     6901 tcagcacttt gggccagccc tggggtatca tccggttgcg aatacctg
//
LOCUS       DAOSOR010000300         6906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_11033_length_6906_cov_5.198219, whole genome shotgun sequence.
ACCESSION   DAOSOR010000300 DAOSOR010000000
VERSION     DAOSOR010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6906
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11033_length_6906_cov_5.198219"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..1430)
                     /locus_tag="PK710_15350"
     CDS             complement(129..1430)
                     /locus_tag="PK710_15350"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOT11155.1"
                     /translation="MGNVRFLTINIVSTVGSVLWCIPAFAQEAPEEGTVQVPAGGGNT
                     TVVVNPPPPTTTTTTTFTPWGVPQGPVEPSEGGSYGFDLSGSGRSESVRGGSNASFTL
                     SDRPSSVPAYHTVRRGDTLWDLCDRYYDNPYAWPKVWSYNPQVENPHWIYPGDRLRMR
                     QSAPTTRAEPTGGGFLRQQHFVAPDTIFLRDYGFVGDESKEVWGTLVGSPNDQMLLSD
                     GNEAYLKIEEGHDVRIGQELTLFRASREPEAGKGKGHVVHIKGTVKVNSWNPKTRIAR
                     ADIIESLDIIERGEKVGPIGRRFDVVPPVRNDRDIWAQITGAIQPRELIGQHQVVFID
                     KGEKDGLRPGNRFFAVARGDRWDQSLEDGRPMASSRVAYRLHSADIERIPDTARGQKF
                     PTEVIGEVRVLRTREDTSVCLVTYTEFELEPGQLLLARRGY"
     gene            complement(1443..2183)
                     /gene="ybgF"
                     /locus_tag="PK710_15355"
     CDS             complement(1443..2183)
                     /gene="ybgF"
                     /locus_tag="PK710_15355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HOT11156.1"
                     /translation="MRIPYRLAWCVVLPALSLGCSGTNASVQRQMEEMRREIYGLRNN
                     QERLDERLTNLELAGAAENTPSKGQRGRPGDLPVIRLAPGEEPPEDTTTPPQQEDEPP
                     TVIRVEGNRTPTVQRGSESAPGSRDAQALRDYDFALELVGKKRYEEALEALSSFLVRY
                     PGHTHADNAMYWRGECYYAMGNFVRAVEQFEGLIARFPYGNKIPDALLKLGLSQRRMG
                     EKEKAAKTFEQLRKNHPSSEAAGKIPRD"
     gene            complement(2273..5443)
                     /locus_tag="PK710_15360"
     CDS             complement(2273..5443)
                     /locus_tag="PK710_15360"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOT11157.1"
                     /translation="MESAQYDREQRAGLSVPRHFTVAGENPLDAVVWEKRTSTIANPD
                     GTIVFQLDDVEVPESWSQLATDIVVSKYFRKAGIGGDPNRGETSVRQVIERLAVTIRQ
                     AGEQFGGYFATAADADAFEAELTHLLVHQMGAFNSPVWFNCGLWHRYGIRGNRGVYAW
                     DESQRKACEIDNAFEHPQCSACFILHIDDDLMGIYEMLKTEAKLFKYGSGTGTNFSAL
                     RARQEKLSGGGTSSGLMSFLDVFDRAAGATKSGGTTRRAAKMVCIDLDHPEIVDFIDW
                     KVREEEKARALIAQGYEADFNGEAYKTISGQNANNSVRVTDTFMDAVEKGGIWKTVAR
                     TTKATVDELDARWLWKKIGDAAWACADPGVQFDSTINRWHTCPNSGRINASNPCSEYM
                     FLDNSACNLASLNLTRFIQQDGQLDLVSFRHAVRVFLLAQEILVDFSSYPTAAIAENS
                     HKFRPLGLGYANLGTLLMLMGLSYDSAAGRTVAAAITAVMTGEAYAVSAEIAAVKGPF
                     EGFAENREPMLHVIGMHRQAAQRIDRAYCPSDLLECAWETWDRALRRGVEYGYRNAQT
                     TVLAPTGTIGLLMDCDTTGIEPDFALVKFKKLAGGGYFKIVNQSVPAALSRLGYTREQ
                     VDRMVSYLSGTATLKNAPHIHRDALITAGLTPHEVERIEEALPGVFELSQAFAPRVIG
                     DAYIRLGVHEPNPSALLRKLGFSARQLEEASDVVVGCMTIEGAPDLREQDVAVFDCAN
                     RCGTKGQRFLRPESHIRMMAAVQPFLSGAISKTVNMPNEATREDVLEIYSLAWRLGLK
                     AVAIYRDGSKASQPLQAKQTKEEKSTKADRMVLTTGAEVVASAEPHGPSVRIRLPKKR
                     TGFTQEARVGGHKVYLRTGEYADGTLGEIFIDMHKEGAAFRSLMNCFSMAISIGLQHG
                     VPLSTFVDQFSFTRFEPHGAVQGHDNVKFATSIVDYIFRVLGVEYLQRYDLAHIPPAP
                     LQHSIEDPSDTKAHKDLTASLPPPESSPQLALPEPSKPSYESALSQSLDEMMGDAPVC
                     DSCGHITVRNGSCYRCLNCGNSLGCS"
     gene            5653..6591
                     /locus_tag="PK710_15365"
     CDS             5653..6591
                     /locus_tag="PK710_15365"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HOT11158.1"
                     /translation="MVWKRRMLWNRVLIESRKPEVCHVAILAGVHGDEQAGFRAFERL
                     LVRLSREPLLQGRVSLIPGNLLAASRKRPTRCAAGQDMNRLFAEPHVLAQQGLVPSGP
                     DWQRAQELTHYLRDVDVLLDLHSTSAPSKPFVVIDKVDDRMRALLAGMPVQRIDYSYR
                     GVLPGTTMEYVERHGGRAITVECGEHRQASGTGAKVAFASAIALLRNLGMLEPGPNPI
                     VTSIPTVRLITRGLIRHPASLQYEPWFKSGAKVAPGQRLAHDRTGEYRAPSAEQLASM
                     LNDNVPDHLIAIMVTAPTRVARVKPHDAYLLGIIDS"
     gene            6713..>6906
                     /locus_tag="PK710_15370"
     CDS             6713..>6906
                     /locus_tag="PK710_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11159.1"
                     /translation="MGISNLWLGLSVSLAVVALSACSGSSEDSEGAAGAGGKILDSGQ
                     TDTEEASTNTDTGTDVVMPET"
BASE COUNT         1441 a         2019 c         1947 g         1499 t
ORIGIN      
        1 ggtagggttt cgtcgggatg cagggatggg ggggtcgtgg gaagcgaggt cttttcgtaa
       61 actggggggg gagggatggt accggccacc cgacaatcat agccgagtac cgctcgggaa
      121 ccagagcctc aataccctcg tcgtgcgaga aggagttggc cgggctccaa ttcgaattcg
      181 gtgtaggtca cgagacagac cgaggtatct tcccttgttc gtaagacacg gacttcccct
      241 atgacttcgg tggggaactt ttgacctcgg gcagtatcgg ggattcgttc gatatcggcg
      301 gaatgcaatc gatacgcgac ccgggaggag gccatggggc gaccatcctc gaggctctgg
      361 tcccaacggt ctccccgagc tacggcaaaa aagcggtttc ctggcctaag cccatctttt
      421 tcgcccttat cgatgaaaac cacctggtgt tgaccgatca attcccgcgg ttgaatcgcc
      481 ccggtgatct gtgcccagat gtcgcggtcg ttgcgtaccg ggggaacgac atcgaaacgt
      541 cgaccgatgg ggccgacttt ttcgccacgc tcgatgatgt cgagggattc gatgatatca
      601 gcgcgggcga tgcgcgtttt tgggttccag gaattgactt tgacggttcc tttgatgtga
      661 accacgtgtc ctttgccctt gccggcctcg ggttcacgcg atgcacgaaa aagcgtgagt
      721 tcttgaccga tgcggacatc gtggccctcc tcgattttga gataagcctc gtttccatcg
      781 gaaagcagca tctggtcgtt gggactgccc acgagcgtgc cccagacttc tttggattca
      841 tcgcctacga atccgtaatc gcgcaggaag atggtgtcgg gagcgacgaa gtgttgttgc
      901 cggaggaatc cgcctccggt gggttcggca cgggtcgtgg gggcgctttg gcgcatacga
      961 agccggtccc ctggataaat ccaatgggga ttttccacct gggggttgta cgaccacact
     1021 ttcggccacg cgtaggggtt gtcgtagtaa cggtcacaca gatcccaaag ggtgtcgccc
     1081 cttcggaccg tgtgataggc aggcaccgaa gaagggcgat cggaaagagt gaacgaggcg
     1141 ttgctaccgc ctcgaacgga ttcacttcgc cccgatccgg ataagtcgaa accataggag
     1201 ccgccttcac tcggctcgac gggtccttgg ggtacgcccc aaggggtgaa ggtcgtggtc
     1261 gttgtggtcg ttggcggcgg cggattgacc acgaccgtgg tatttccccc tcccgcggga
     1321 acttgaacgg ttccttcctc gggggcctcc tgggcgaagg cgggtatgca ccacagcacg
     1381 gatccaactg tcgagacgat attgatggtc aggaatctga cgtttcccat caccttactc
     1441 cctcaatccc tcgggatttt accggcggct tcactgctgg ggtggttttt tctgagttgt
     1501 tcgaaggttt ttgccgcttt ttctttttca cccatccgac gttgggatag ccccagtttc
     1561 aacaaggcgt cgggaatctt atttccataa ggaaaacgcg ctattaggcc ttcgaactgt
     1621 tcgacggcgc ggacgaaatt ccccatggcg tagtagcatt cgcctcgcca gtacatcgcg
     1681 ttatcggcat gggtatgtcc gggatagcgg accaagaagc tggacaaggc ttccaaggct
     1741 tcttcgtatc gtttttttcc taccaattcg agggcgaagt cgtagtcccg cagggcctgg
     1801 gcgtcgcgag atcccggcgc ggattcgcta cctcgttgaa ccgtgggagt tcgattgcct
     1861 tccacacgaa tgaccgtggg cggctcatct tcctgttggg gcggtgtggt cgtgtcttcc
     1921 ggcggttctt cgcccggagc aagccgaatg acgggcaggt ccccgggtcg gccgcgttgt
     1981 cctttggacg gcgtattttc ggcggctcca gccagttcga gattggtcag ccgctcgtcg
     2041 agccgctcct gattgttccg aagtccgtag atttcacggc gcatttcctc catttggcgt
     2101 tggacggaag cattcgtgcc tgaacagccg agagataggg ctggcaggac gacacaccaa
     2161 gcaaggcgat acggaatcct catccgatcc gaagcctacg gcgaatggga gggatgggcc
     2221 aatttcgcgg ggaaagaatg ggggagaagc tccgcgccgg gtcgcggcct tttcaggaac
     2281 aacccaggga attgccgcaa ttcaggcagc gataacagga accatttctt acggtgatat
     2341 gcccacagga atcgcaaacc ggcgcatcgc ccatcatttc gtcgagagat tggctcaagg
     2401 cactttcgta cgacggtttc gatggttcgg gcaatgccag ttgaggactc gattccggag
     2461 gcggcaacga agcggtcaag tccttatgcg ctttggtatc cgatgggtct tcgatgctgt
     2521 gctggagcgg agcgggaggg atgtgagcca ggtcatagcg ttgcaggtat tccacgccga
     2581 gcacgcgaaa gatgtagtcc acgatggagg tggcaaactt cacgttgtcg tgtccttgca
     2641 ccgccccatg cggctcgaag cgtgtgaaag agaattggtc gacgaaggtc gacaagggca
     2701 cgccatgctg caatccgatc gaaatcgcca tggaaaagca gttcatcaac gagcggaagg
     2761 cagcgccttc tttgtgcatg tcgatgaaga tttcgccgag cgtaccatcg gcatattcac
     2821 ccgttcgaag atagacttta tgccctccca cccgcgcttc ctgcgtgaat cccgtacgtt
     2881 ttttgggaag gcgaatccga acgctggggc cgtggggctc ggcactggcc accacttccg
     2941 cgccagtcgt gagcaccatc cggtcggctt tcgtgctttt ttcttccttg gtttgcttgg
     3001 cttgcaaggg ctgcgatgct ttcgacccat cgcgataaat cgccacggct ttgagtccca
     3061 accgccaggc cagcgagtag atttccagaa cgtcctcacg ggtggcctcg ttcggcatgt
     3121 tcaccgtctt ggagatcgca ccagacaaga aaggctgcac cgccgccatc atgcggatat
     3181 gcgattctgg gcgtaaaaac cgttgtcctt tcgtcccgca tcgattcgca caatcaaaga
     3241 cggcaacatc ttgctctcgc aagtccggtg cgccctcgat cgtcatgcat cccacgacga
     3301 catcgcttgc ttcttcgagc tgtcgtgcgg aaaaccccag tttgcgaagg agcgcggagg
     3361 ggtttggctc gtgaacgccg agccggatgt aggcatcacc tatgacccgc ggagcgaacg
     3421 cctgggagag ttcgaaaacg ccggggagtg cctcctcgat acgttcgacc tcatgaggcg
     3481 tcaaaccggc ggtaatcaga gcatctcggt gaatgtgggg cgcatttttt agcgtggccg
     3541 taccggacaa atacgacacc attcgatcca cttgttcgcg ggtatacccg agacgcgaaa
     3601 gcgcggcagg aaccgattga ttgacaattt tgaagtagcc ccctccggcc agtttcttga
     3661 acttgaccaa agcaaagtcc ggctcgatgc ccgtggtatc acaatccatc agcaatccga
     3721 tggtgcccgt gggagctagc accgtggttt gtgcgttgcg atacccgtat tccactcccc
     3781 tacgaagcgc gcgatcccac gtttcccacg cgcattccaa taaatcactt ggacaatagg
     3841 cccgatcgat ccgctgggcg gcttgcctat gcataccgat gacgtgcagc atcggctcgc
     3901 ggttttcggc aaagccctca aacgggcctt tgaccgcggc gatttcggcg gaaaccgcgt
     3961 aggcctcgcc cgtcatcacc gccgttatcg cggcagccac ggtccgacct gctgccgaat
     4021 cataagaaag gcccatcaac atcaacaggg tgccaaggtt tgcgtaacca aggccaagtg
     4081 ggcgaaattt atgagaattt tcagcaatcg ccgcggtcgg ataggatgaa aaatccacca
     4141 agatctcctg cgcgagcaga aagacccgaa ccgcatgccg aaaggacaca aggtcgagct
     4201 gcccgtcttg ctggatgaaa cgggtcaggt tcagcgatgc gagattgcag gcggaattat
     4261 cgagaaacat atactccgag cacggattcg aagcgttgat gcgaccgctg ttgggacacg
     4321 tatgccaacg attgatggtc gaatcgaatt gcaccccagg gtccgcgcac gcccacgccg
     4381 catcaccgat ttttttccaa agccagcgcg catccaactc gtcgactgtc gccttcgtcg
     4441 tacgagccac cgttttccag atccctcctt tttccaccgc atccatgaaa gtatcggtga
     4501 cgcgcaccga gttgttggcg ttctgcccac tgatggtctt gtacgcttcc ccattgaagt
     4561 cggcctcata tccctgcgca atcaaagcgc gcgccttttc ctcctcacgc actttccaat
     4621 cgatgaaatc gacaatctca gggtggtcga ggtcgataca caccatcttg gcggcccgac
     4681 gagtcgttcc cccggacttg gtagcccccg cggctcgatc gaatacatcc aaaaaactca
     4741 tcaagcccga actggtccct ccccccgaca gcttctcctg tcgcgctcgc aacgcgctga
     4801 aattcgtccc cgttcccgaa ccgtacttga aaagcttagc ctccgttttc agcatctcat
     4861 agatgcccat caagtcatcg tcgatgtgca ggatgaaaca ggccgaacat tggggatgct
     4921 cgaaagcatt gtcgatttca cacgcctttc gttgggattc atcccaggca tacaccccac
     4981 gattgccccg aatcccataa cgatgccaca gcccacaatt gaaccagacc ggcgaattga
     5041 aagcccccat ctgatgaacc aacagatgcg tcaactccgc ctcaaaagca tcggcatccg
     5101 cggccgtggc aaaatacccg ccgaattgct cccccgcctg ccgaatcgtc accgccaaac
     5161 gctcgatcac ctgcctcacc gaagtctccc ctcgattggg atctccccct atcccggctt
     5221 tcctaaaata cttggatacc acgatatccg tggcaagctg cgaccacgac tccggcacct
     5281 ccacgtcgtc gagttgaaac acgatcgtcc catccggatt ggcaatcgta ctcgtacgct
     5341 tctcccacac caccgcatcg agtggattct cacccgcgac agtgaagtgg cgcggaacag
     5401 agagacccgc cctctgttcc ctgtcgtatt gcgcgctttc catatttatc accatttccc
     5461 ttgtttcaga ttccagaaac cgcccatggc cgaaagggat tggcaagcgg ttcctatagg
     5521 gggaggaagg gctattacac tactggttgt ggttactcaa gcaccaacca caacataatg
     5581 aggtttccac cctaagtcgt cgatcaacaa agcgcttttc ttctccgccg ctctcttgca
     5641 aggtcccccc tcatggtctg gaaacgtcgc atgctctgga atcgggtgct gatcgagagc
     5701 cgtaaaccgg aagtctgcca cgtggcgata ttggcggggg ttcatggcga tgagcaggct
     5761 gggtttcgcg cgtttgaacg attgctggtt cggttgagcc gtgagcctct gctgcaagga
     5821 cgagtgtcgt tgattcctgg caacctgctc gccgcttcgc gaaaacggcc tacccgatgt
     5881 gccgccggac aagacatgaa tcgcctcttc gccgaaccac acgttctcgc ccagcaaggt
     5941 ctcgtaccgt ccggacccga ctggcaacga gcccaagaac tcacccacta ccttcgcgac
     6001 gtggacgtgc tgctcgacct tcattcaacc tcggcgccaa gcaaaccctt tgtcgtcatc
     6061 gacaaggtgg atgatcgaat gcgcgcgttg ctcgccggaa tgcccgtgca gcggatcgac
     6121 tacagctacc ggggagtgct tccaggaacg actatggagt acgtcgaacg tcatggtggt
     6181 cgtgccatca cggtggagtg tggagagcat cgtcaagcct ccggtacggg cgcgaaagtg
     6241 gcatttgcca gcgcgattgc cttgctgcga aaccttggca tgctcgagcc gggacccaat
     6301 ccgatcgtca cctccattcc caccgtgcgt ttgataacgc gagggttgat tcgacatccg
     6361 gcttccttac agtacgaacc ttggttcaaa agcggggcga aagtcgctcc cggacaacgg
     6421 ctcgcccacg atcgcaccgg agagtaccga gccccctctg ctgaacagct cgcctcgatg
     6481 ttgaacgaca acgttcctga ccacctcatc gccatcatgg tcacagctcc cacccgcgtg
     6541 gcgcgggtca agccccatga cgcatatttg cttggaatta tcgatagtta gaagaattcc
     6601 ccctcgacgt cgatcggacc atttccatca tcacccctgg cacgagagtt ggacgagcga
     6661 gtgggggaag ggtatggttg ttccagatca gtcgagaacc gcgaggtgca taatgggtat
     6721 atcgaatctt tggcttgggc tatccgtttc gcttgccgtc gttgcgctaa gcgcctgcag
     6781 cggtagctcg gaagactcgg aaggggccgc gggagctgga ggaaaaatcc tcgactcagg
     6841 ccagaccgac acggaggaag catcgacgaa tacggatacg ggaaccgatg tcgtgatgcc
     6901 cgaaac
//
LOCUS       DAOSOR010000301         6889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_11069_length_6889_cov_4.223295, whole genome shotgun sequence.
ACCESSION   DAOSOR010000301 DAOSOR010000000
VERSION     DAOSOR010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11069_length_6889_cov_4.223295"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="PK710_15375"
     CDS             <1..698
                     /locus_tag="PK710_15375"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HOT11160.1"
                     /translation="RIEREKGEPITFGDASQQAVLEHVGIHHARILVVVISDAAAAGR
                     AVEVARRINPGIHIVTRTRFITEVESLHAIGANEVIPEDLESSVEIFTRVLTHYLVPR
                     DRIDQLAADIRAGGYEKLRSPSVSSETLQSCKVPIPDVQVSACSVAEGSELAGRTLAQ
                     LDLRNRHGATLLAIRRDDQTIANPKGSTRIEAKDTLLVLTTAGKAGHISRVCAGFPQE
                     QAVQPDPPSHGSS"
     gene            823..2265
                     /gene="cls"
                     /locus_tag="PK710_15380"
     CDS             823..2265
                     /gene="cls"
                     /locus_tag="PK710_15380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOT11161.1"
                     /translation="MNITSMHDFLLWLDLVAFIFFLLALLSIPSVLVQRTGRPLAALA
                     WILTMVAFPPVGMILWWAFGRRHLKRQRRRRRASTGEIADKIQTVISGAAAPAYRAIP
                     LLPLRRLSEEYRQGVFAPVGGNEVQLLVDAAQAYPAMLDAIEAAQHHIHLLFYIWNHD
                     DTGRHFLRLLERKARQGVEVRLLCDAIGSWGMTPRAAKELIAAKGQVARFLPPGLTAG
                     VPTLNFRNHRKILVVDGKVGFTGGINVGDEYAGAWKDLALQVRGPAVLQLQEVFADDW
                     FFSTGEDLADLEHFGPHRPWKGQATCQLVASGPDCRYEQMQDVLFMAINEAKERVFIV
                     SPYFIPNPPILAALRSASLRGIDVRIMVPGRSDVRISRWASRSFYSDLLDVGIRLFEY
                     MPGILHAKAVLFDRELSLVGSANINNRSFRLNFEASMVVGDPTLNHAIASLFESDMAQ
                     SNEIHLDEVLQRPWRTKVLDGAAHLLSPIL"
     gene            3106..5793
                     /locus_tag="PK710_15385"
     CDS             3106..5793
                     /locus_tag="PK710_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11162.1"
                     /translation="MRPFILTPGLKANDKLGIRTAAEAFNEVFREAGLLPEGELLEIL
                     DSVADKNTVAAYRAQGRPFISLGATSVLQDAANHDLTASEKTSAISLPYDGWRVISRN
                     GNLYIFGRDVGYTSGERTSRNGFSRGTANGVYDFLEECVGARWLLPGSIGLDVPKANE
                     IAIGAIDYTGTHRFAARVLSSVTTDEDIDQTAIIEKWRAALRQDGPVKIGANHAWWQV
                     INRGYDKNDDGIQSGPFNHHHVDGPNNDNVKSDAVKSAYKAHPGWFALVCDENGNNCI
                     RKEPTTNHDKLESTHPEVQQFFADQANVECNDLKPFSLSPSDGSKTFSMSPESNAFLG
                     EVPKGHLLSGRTLRTPLVLDWYEQVIARADTTCEVVPGKPLMFSGFLYSEYRFPPLGW
                     KSTGAYAPKALSASFVPFYVENGGYSMLRPDVRKVMVYNLCGWESLIPSLSSSDPRTQ
                     EYRGMENKEWYYYGNNFQLFMGAKSEVGGKDRSYAGSSGMVTPVATDAIDFLFALLLE
                     TGVSGIDHIYGARSFSNAGLSNYVIARKMIEPERPALDIRDEWIERAYGSAAAGMKAF
                     YDQLIVDYSTYYNDPAWQSNDDQDENIDDPVEDENETGTKGPVQAGVINCDLLHELVN
                     KYPQWENLFLSAAAQLSPEEQAGKQGQRLSLLRMNLQVLAWRLHQVDDAFTAQLAHDD
                     ATVDDLLSGRFEHDRPIEERLATSFELFPELTNTFQNDRNFKWDELPNRLPPIALVSS
                     VGSNKGVLPAPLSSDKTVLIYFNRPATVVFEVSNEMKQSPHMATYEVHDKTGLRVGRG
                     LLYAGAKIELNGARADAFTITFTLRKGTNFKVDSSNNAYVGDYKVATNELRLLDHADQ
                     AVGVLYPHTRLQLTENQGRVSIRMANPKDTLGANKFICR"
     gene            5982..>6889
                     /locus_tag="PK710_15390"
     CDS             5982..>6889
                     /locus_tag="PK710_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11163.1"
                     /translation="MKYSTFSPILGTLLLALGLGSCGDSGSSDNHGSAGAGGTANGGA
                     AGSGGTPEGGIEDGANPTNACGDDPNGAAPLVLGGPGASGLTIVIPADASSQLKRAAT
                     ELRDDIELATGTTLEIVRDDAASAPAGNIISLGATKQAQAAGFSAKNLEFDAFRIVRK
                     DNDLFIYGQDDLEDPLVVQSFGTVNGVHVFLESCLDVRWLLPGPTYKDVPERKTFEIG
                     AIDKIVTPRFKNRVIHEFIIDGFVYNHFYDWTRQLRFHGENPRFTVGSHSWGRIAGAH
                     VLPDESHPNSRLAGVDLPVSEMSCDE"
BASE COUNT         1542 a         1855 c         1919 g         1573 t
ORIGIN      
        1 ttcgcatcga gcgagaaaaa ggtgagccca tcacgtttgg cgatgcttcc cagcaagccg
       61 tgctcgaaca cgttggcatc caccatgctc gcatcttggt ggtcgtgatt tcggatgcag
      121 cggctgccgg tcgcgcggtc gaagtggcgc gacgtatcaa ccccggtatc catatcgtaa
      181 cgcgcactcg cttcattacc gaagtggagt ccttgcacgc cattggagcc aatgaagtga
      241 ttcccgagga cctcgaatca tcggtggaga ttttcactcg tgtgctcaca cactatttgg
      301 ttccgcggga tcgcatcgac caactcgctg ccgacattcg cgcgggagga tacgaaaaac
      361 tccgttcccc atccgtttct tccgaaactt tacagtcctg caaagtgcct atccccgacg
      421 tgcaagtatc ggcttgctcg gtggccgaag gctcggagct ggcgggtcgg acgttggcgc
      481 aactcgatct tcgcaatcgc catggggcaa cgttgcttgc cattcgacgc gacgatcaaa
      541 ccattgccaa tccaaaggga tcgacacgaa tcgaagcgaa agacacgtta ctcgtgctta
      601 ccacggcagg aaaagccggg catatatcgc gagtttgcgc gggtttcccc caggagcaag
      661 ccgtccagcc agacccgcca tcgcatggtt catcgtgatg gacttcccag ccgcctcggc
      721 cgaagcgtcg tgcagcgtca tcccaccatg gcttcaggtt actgctgata tttttgatac
      781 gctgcgtggc cgcgccacga aagacaccaa gggccaccga agatgaacat cacttcgatg
      841 catgattttt tgctctggct cgacttggtg gccttcattt tctttctgct cgccctgctg
      901 agcatcccaa gcgtccttgt gcaacgaacc ggtcgcccct tggccgccct ggcctggatc
      961 cttaccatgg tcgctttccc tcccgtggga atgatcctct ggtgggcgtt tggacggcga
     1021 catctcaaac gacaacgccg tcgccgtcga gcctcaactg gagaaattgc tgataaaatt
     1081 caaacggtta taagtggcgc ggccgcacct gcctatcgtg cgattccgct gctccccctt
     1141 cggcgcctct cggaagagta tcggcaaggc gtctttgcac cggttggtgg caacgaagtc
     1201 caattgctgg ttgatgcagc ccaagcctat cccgccatgc tcgatgccat cgaggcggcg
     1261 caacaccaca tccatctgct tttttatatt tggaatcacg acgacacggg tcgccatttt
     1321 ctgaggctat tggagcgaaa agccagacaa ggcgtcgagg ttcgcttgct gtgcgatgcc
     1381 attggttcct gggggatgac cccacgggct gccaaagagt tgatcgcggc caaggggcag
     1441 gtagcgcgct ttttaccacc agggcttacg gccggcgtgc cgacgctcaa tttcagaaat
     1501 caccgaaaaa ttcttgttgt cgatggtaag gtcggattca cgggcggaat caatgtggga
     1561 gatgaatatg cgggcgcatg gaaagacttg gcccttcagg taagagggcc agccgtcctg
     1621 caactccaag aagtattcgc tgatgattgg ttcttttcga caggagaaga cctggcggat
     1681 ttggagcact tcggtcctca cagaccatgg aagggacaag ccacttgtca actcgtcgcc
     1741 agtggacctg attgccgata cgaacaaatg caagacgtgc tgttcatggc aatcaatgaa
     1801 gccaaagaac gagtgttcat cgtttcgccg tacttcatcc ccaatccccc aatcctcgcg
     1861 gctttgcgta gtgcttcgct tcgaggcatc gatgtccgca tcatggttcc cggtcgcagt
     1921 gacgttcgaa tcagtcgatg ggcatcccga tccttttatt cggacctttt ggacgttggt
     1981 atcagactgt tcgagtacat gccgggaatc cttcatgcca aagcagtcct tttcgatagg
     2041 gagctatccc tcgtgggaag cgccaacatc aacaatcgga gctttcgact caacttcgaa
     2101 gccagcatgg tggtgggaga ccccacgctc aaccacgcca tcgcctcctt gttcgaatcc
     2161 gatatggctc aatcgaacga gatccatctc gatgaagtgc tgcaacgacc atggcgcacg
     2221 aaagtcctcg atggggcagc gcatctgttg agccccattc tctagcgttt ccgatgattt
     2281 cccctgttcg agtgacgggc ggcgatgtac gggcgttagc ggaaaaacct ccttgggact
     2341 ttgctccggt gcctacgtca cggctgcgcc aacaccacgc ggaggcactc ccccatcggc
     2401 tacgaaagct cccgccgagg cggatttaag agtcaactcg ccgtcatggt ggtatagtca
     2461 aacgttccgc caggagcggg taagctcgcc tcacgccaga accaggcgcg atacgagttc
     2521 gaagccgtga aacgagtggt ctgaatgcag tctagctggt gtctccgcaa cagcgtgcgc
     2581 cccacacgtc gggtgcccga agatgttttc gggaacgtga ggtgacttat gcgacgaagc
     2641 cttgggttgg cgattctgct tccgtttggt gtggctctga tgggctgcgg cgaggactcc
     2701 agtgaatctg gaggggacct ggtgggaggc tcgggcggga acccctctga cgcgagcata
     2761 accgacgcct ttgaggcgag cacaactgac gcctctgacg cgagctcaac cgacgcctct
     2821 gacgcgagcc caaccgactc gaacacaaca gacacactcg actcgaacac aacagacaca
     2881 tctgactcga gcacggaatc ggacgcgtgt gaggattcac aggagggctt ccccgtttat
     2941 cccgggacgt tctgtgtagg cattgacccg cagacgggaa agccaagttc tgcggtcgat
     3001 tccccgggat gtgaaaaacg ctggctgcct gatcctattc ttcatgagca aaagtgcgga
     3061 gccggcaccc cctttgtgtt gcttcgctct tccgatgtgg cggcgatgag gcctttcatc
     3121 cttacgccgg gcctcaaggc gaacgacaag ctagggattc gcacagcagc cgaagccttt
     3181 aatgaggttt ttcgtgaggc gggtttgcta cctgagggag aattgctaga gattttggat
     3241 tcggtggccg acaaaaatac ggtcgctgcg tatcgggccc aggggcgacc gttcatcagt
     3301 ttgggggcca cgagcgtgtt gcaagatgct gcgaatcatg accttaccgc gagcgaaaaa
     3361 acaagtgcaa tatcgcttcc ttacgatggc tggcgggtga tttcccgcaa tgggaatctg
     3421 tacatttttg gtcgggatgt ggggtatacc tcgggggaac gaacgtcacg aaacggcttt
     3481 agtcgaggga ccgcaaacgg tgtctatgac tttctggaag agtgtgtggg ggctcgttgg
     3541 cttttgcctg gctcgattgg cttggacgtt cccaaagcca acgagattgc cattggcgct
     3601 atcgactaca cggggactca ccgttttgca gcgcgcgtcc tgtccagtgt gaccaccgat
     3661 gaggatattg atcaaaccgc catcattgaa aaatggcgag cggcattacg tcaagacgga
     3721 cctgtcaaga taggggcaaa ccatgcgtgg tggcaggtca tcaatcgagg ttatgataag
     3781 aacgacgatg gaatccagtc cgggcctttt aatcatcacc atgtcgacgg gcccaacaat
     3841 gacaatgtca agagcgatgc cgtgaaaagt gcctacaagg ctcatccggg gtggtttgct
     3901 ctcgtctgcg atgagaatgg caataactgt atccgcaaag aaccaacgac caatcacgat
     3961 aaattagaaa gcacccatcc agaggttcag cagttttttg cggatcaagc caatgtggag
     4021 tgcaacgatc tcaaaccctt ttcactttcg ccaagcgacg ggagcaaaac cttcagcatg
     4081 agcccggaaa gcaacgcgtt tttgggagag gtgcccaagg gccatctgct cagcggtcga
     4141 acgctgcgta ctccgctcgt tcttgactgg tatgaacagg tgatcgcgcg agcggatacg
     4201 acgtgtgagg ttgttccagg aaagccgctc atgttctctg ggttccttta ttcagagtat
     4261 cgtttcccac cccttggatg gaaaagcacg ggagcgtatg ctcccaaagc cttatcggcc
     4321 agttttgtcc cgttctatgt ggaaaacggt gggtattcga tgcttcggcc tgatgtgcgt
     4381 aaggtcatgg tttacaacct ttgcggttgg gagagcctca ttcccagtct atcgtccagt
     4441 gacccccgca cccaagagta tcgcggaatg gagaataagg aatggtatta ctatgggaac
     4501 aacttccagc tctttatggg tgcaaagagc gaggttgggg gcaaggatcg cagttatgca
     4561 ggttcgtcgg ggatggttac ccccgtggcc acggatgcga ttgatttctt atttgcgttg
     4621 ctgctcgaga caggtgtttc tggaatcgac catatctatg gggctcgctc ctttagcaat
     4681 gctggtcttt ccaactatgt cattgctcga aagatgattg agccggaacg acccgctttg
     4741 gatatccgcg atgagtggat tgaacgagct tatggctccg ccgcggcggg tatgaaggcg
     4801 ttttatgatc agcttattgt tgattacagt acctattaca acgatccggc ttggcagagc
     4861 aatgatgacc aggatgaaaa catcgatgac cccgtggaag acgagaatga aacgggaacc
     4921 aaggggccag tacaagctgg ggtgataaac tgcgaccttt tgcatgaact cgtgaataag
     4981 taccctcagt gggaaaacct gtttctttct gcagcggctc agctttcgcc cgaggagcaa
     5041 gcgggaaagc aagggcaaag gctcagcctc cttcgaatga atcttcaggt attggcttgg
     5101 cgtctccacc aggtggacga tgcttttacg gcgcagcttg cccatgacga tgccacggtg
     5161 gatgacttgt tgagtggacg ctttgagcac gatcggccca tcgaggaacg cttggccacc
     5221 agctttgagc ttttccctga actcaccaat acgttccaga atgatcgcaa ctttaaatgg
     5281 gatgagttgc ccaatcggct tcccccgatc gctttggtgt ccagtgttgg ctcaaacaag
     5341 ggggttcttc ccgcaccctt gagcagcgat aaaactgtgc tcatttattt caatcgacct
     5401 gccactgtgg ttttcgaagt ctccaatgaa atgaaacaaa gccctcacat ggcgacctat
     5461 gaggtacacg ataaaactgg gctgcgagtg ggccggggat tgctctacgc tggggcgaag
     5521 atcgaactga acggtgcgcg ggccgatgcc tttacgataa catttacctt gagaaaaggc
     5581 acaaatttca aggtagatag ctcaaataat gcttatgtgg gtgattataa ggtcgcaacc
     5641 aatgagctac gattgctcga tcatgcagac caggcagtgg gggtattgta tccccatacg
     5701 cgacttcaat tgaccgaaaa ccaaggtcga gtaagcattc gcatggcgaa tccaaaagat
     5761 accttgggtg cgaataagtt catctgccgc tagagattga tgcactgtag tggataaaag
     5821 gctgtttaac cggatagctg aactttcgta ccggagccac gcttgctccc gttctgcttg
     5881 ccaaaccact gggtcacttc atatacaatg ttcggtgtcg ccgctacggt tgcgtctgtg
     5941 ctaggatgag aaattccctt ggccgttatt tccggagtcc catgaaatac tcgacctttt
     6001 cgcctatcct tggaacgctt ctgcttgcgc ttggccttgg ttcctgcggc gactcggggt
     6061 caagcgacaa ccatggctca gccggtgccg gtggcaccgc aaacggaggc gcggcgggaa
     6121 gcggtggcac gcccgaaggg ggtatagaag acggtgcaaa ccccacaaac gcctgcggtg
     6181 acgatccaaa cggcgccgcc cccctggtcc tcggcggccc aggggcttcc ggtctcacta
     6241 tcgtcatccc tgcggatgca tcctctcagc tcaagcgtgc tgccacggag ttgcgtgacg
     6301 acatcgagct tgcgacagga acaacgttgg agatcgtgcg ggatgatgct gcctccgcac
     6361 ccgcaggcaa catcatcagt ctgggtgcca cgaaacaagc gcaagctgcc ggattttcag
     6421 ctaaaaacct cgaattcgac gcctttcgca tcgtgcgaaa agacaacgat ctattcatct
     6481 atggtcagga tgacctcgaa gacccgctcg tcgtccaaag cttcgggacg gtcaacggag
     6541 tacacgtttt tcttgaaagc tgcctcgatg ttcgctggct tctgcctggt ccgacctata
     6601 aggacgtccc cgagcgaaag acatttgaga taggtgccat cgacaagatc gtcacaccca
     6661 gattcaaaaa ccgggtcatc cacgaattta tcatcgacgg ttttgtctat aaccactttt
     6721 atgattggac gcggcaactt cgctttcacg gcgagaaccc caggtttacg gtaggctccc
     6781 actcttgggg aaggatcgca ggcgctcacg tgttgccaga tgagtctcat ccgaattcac
     6841 gacttgccgg cgttgacttg ccggtaagcg aaatgtcctg tgatgagtt
//
LOCUS       DAOSOR010000302         6888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_11070_length_6888_cov_4.630762, whole genome shotgun sequence.
ACCESSION   DAOSOR010000302 DAOSOR010000000
VERSION     DAOSOR010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11070_length_6888_cov_4.630762"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="PK710_15395"
     CDS             complement(<1..853)
                     /locus_tag="PK710_15395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT11164.1"
                     /translation="MTVLFVYRRDVLVRVAARAAQKAGLPFESVEASSCERVDPRVRV
                     LVLPVEDLFRLSPGLSPSTKVIAYGRASERGQHSMAGMRYLVSPFRVDDLAGLLESIV
                     PRVAPSPVQGRMPPVAEQETQKTIEPPDRAARPQTTAPPEKPEKYKILAVDDDRIIRR
                     LYERVLTEVGYEVLLAEDGEDAWQKIASFAPDLVISDVEMPRVDGYELCRRVKEYPQW
                     AQTPFIICSSLSGGRDVMRAFESGTDDYLIKPINEDELLGRVAEMLASIEQGGRELIL
                     VVDDSRIV"
     gene            complement(969..2192)
                     /locus_tag="PK710_15400"
     CDS             complement(969..2192)
                     /locus_tag="PK710_15400"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOT11165.1"
                     /translation="MLGACSAPSVRPQPPSAAPAAPVAPASLPKAPSLELVESWPSET
                     SLDDPLIRDAAVAWVDLIDSARERIDISEFYAKSAPNSALERVIAALLRAADRGVRVR
                     MIVDTKLLGDNHEVPERLEAHPKIEVRRYDLGAIMGGVQHAKYFVVDDELAYLGSQNF
                     DYRSLEHILELGVVIRVRPVVDALRATFEDDWRMAAGEPRSESKPFDACASDIEYGGK
                     PVRVCAALSPKGFLIDEALWDLPRLLQLIGEARKTITVQLLDYATVNYDRSSFTELDD
                     ALRSAAARGVHIRMMVADWSKRAKSLEPLQALQRVPNIDVRFVTIPPHSSGFIPHARV
                     IHAKAMVVDGLNSWVGTSNWSGDYFYKSRNVGIVVEGASFAQDVARFFETTWDSNYAY
                     DLDPDAKYEPPRIKE"
     gene            2617..4158
                     /locus_tag="PK710_15405"
     CDS             2617..4158
                     /locus_tag="PK710_15405"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HOT11166.1"
                     /translation="MATSQPLAAQAGLRMLASGGNAVDAAIAAAAALTIVEPVSCGLG
                     GDAFAMVWDGHRLHGLNASGTAPASWSLTYFTRRYGSVFPQRGADTVTIPGAVAGWAA
                     LHNRFGRLSLSDCLEPAIELACQGFGVTPIVQTKWAAQVDALQSQPGFASTFMPHGRA
                     PFVGERFVLAQAAQTLRRIGQSAGRDAYEGQTAEKIVGFLREHGGRMTLADLREFQPI
                     WVEPISREYHGFALHELPPNGQGIAALITLGLLDHFDFPSLAPDTVTFFHLPIEAMKA
                     AFSQVYRHVTDDRHMIIEPVALLDDARLAQLASRIRLDAATDFDQGLPPAGDTVYLAT
                     ADASGMMVSFIQSNYMGFGSGLVVPDIGVSLQNRGCGFCLEPNHPNVVAPRKRPFHTI
                     IPAFLTRNGAPQMCFGVMGGNMQPQGHVQTLLRMIDHHQNPQTACDAPRWKVQRGLQI
                     SLEPSIPMPVRQGLADLGHQIVHETDSYMDFGSGQFIWRLSDDLADGYMAASDHRRDG
                     CAVGF"
     gene            complement(4184..>6888)
                     /locus_tag="PK710_15410"
     CDS             complement(4184..>6888)
                     /locus_tag="PK710_15410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT11167.1"
                     /translation="LGISARAESLESLADVAMAMVPVVSGLAQGDFDPFGTAGGFFVQ
                     AVGVLEEAFLSLEAGDGLNVDALRSCANKITSMTSCLMPASAELSETAASDLQNHKNI
                     NKINNIDRSEAESESWGPWDPPYPDMESDYVEEVAAHIDHLQQGLVQLASGGSQPDLI
                     NDLFRSSHSIKGQSGQMSVRPIEKVAHKLEDVLALVRESKLVLSASSVEVLLSVIDSL
                     ASMLEQLRQTRCIVHPIGKETERMDGVIQGTWESKSQSDDRSVGRSSPVIVPTTLSSN
                     ASSPEVSPMVLEAKGPAPSVGFDTSATSHPVTDSAPKETRASSSSLSKAQYLRVDFEK
                     VDRALNRVGDLFINKIRLNDTAELYAELMFHVRTLQNVLGRAHGSSEEGVWLTREDAV
                     HLQEGLAALELEMEQAADQLGSAMGETDMISSDLRDQVMEMRMVPVNTVLGRLSRVVF
                     DALQKENRAAGASPKRARLEVIGADAEIDKMMANMLEVPLVHIVRNAVAHGIESVAER
                     EAAGKVAEGTIRIRAQQQGSRFVIETTDDGRGMDPAVLGKVALAKGIATKEALEGMTE
                     REVLGLIFRPGFTTAERADDLKGRGVGLDEVMDKIRAIKGSVDVSSVMGKGSRIELSL
                     PLTLAITTVVLGELDTDTLAIPMSAVERVVHLSESDIEAMGDAEIFTLLGETVPLVRL
                     DEMLGMGRPASKRPDSCYVTVVRLGDKRMGLALDRMRGQREVVVKGLGTLLKQVPFVA
                     GSTLLGDRCVLIVDPAEIIDSVGKPGVRVVAPSASVPSSSKRCALLVDDDAMTRLALR
                     RVFEQAGLEVYEAGDGMEGLMMAQQRQYHLVSTDVVMPRMDGYELTRRLRALPAFHRT
                     PILMVTSKDLEVDRRAGFEAGVDRYVTKPFERSELLQLVEEMIH"
BASE COUNT         1508 a         2305 c         1700 g         1375 t
ORIGIN      
        1 gcacgatgcg agaatcatcc accacgagaa tgagttctct gcccccttgc tcgatcgaag
       61 caagcatctc tgccacccgc cccaacagct catcctcatt gatgggtttg atcaaataat
      121 catccgtgcc cgattcgaac gcgcgcatga cgtcgcgccc gccagatagg ctcgaacaga
      181 tgatgaacgg cgtttgcgcc cactggggat actctttcac gcgacgacac aactcgtagc
      241 cgtcgacccg cggcatctcc acatcgctga tcaccaggtc cggcgcgaaa gaagcaattt
      301 tctgccacgc atcttcccca tcttccgcca agagcacctc gtaccccact tcggtgagca
      361 cccgttcgta caaacggcga atgatccggt catcatcgac agcgaggatt ttatattttt
      421 caggcttttc gggcggggcg gtggtttgcg gtctcgccgc tcgatcgggc ggctcgattg
      481 ttttttgtgt ttcttgctca gccaccggcg gcattcggcc ctgaaccggt gatggcgcaa
      541 cccgtggcac gatggactcc aagagtcccg caagatcatc gacccgaaaa ggagacacga
      601 gataacgcat gcctgccatg ctgtgctgcc cgcgttccga agctcttcca tacgcaatga
      661 ccttggtgga gggagacaac ccgggactga ggcgaaacag gtcttcgaca ggcaaaacca
      721 gcacgcgcac tcgtggatca actcgttcgc aggagcttgc ttccacgctc tcgaacggca
      781 gcccggcttt ctgcgccgcg cgagccgcca cgcgcaccag cacgtctctg cgatacacga
      841 acagcacggt catgtcagaa ccgtatcaca acccaatgaa tcagcgggat gccacaaaca
      901 agcctcggac gccgttcgat atcacgccat cgtgggagaa ggcatggtcc gcgggcctcg
      961 atctcccttc actctttgat tcgaggcggc tcgtacttcg cgtcgggatc gaggtcgtag
     1021 gcgtagttac tatcccaggt tgtctcgaaa aatcgggcca cgtcttgggc aaaactcgct
     1081 ccttccacca cgatccccac gttccgactc ttataaaaat aatcccctga ccaattcgag
     1141 gtccctaccc aggagttgag cccatcgacc accatcgctt tggcgtgaat gacccgcgca
     1201 tgagggatga accccgagga atgaggggga atcgtaacga atcgcacatc gatattgggc
     1261 acgcgctgca aggcctgcag cggttcgagg gacttggcgc gcttggacca atccgccacc
     1321 atcatccgga tatggacacc ccgcgccgcc gcggaacgta acgcatcgtc gagttccgta
     1381 aacgaggatc gatcgtagtt gaccgtggca taatccaaca attgcaccgt gatcgtcttg
     1441 cgggcttccc cgatcagttg cagaagacga ggcaagtccc agagcgcctc gtcgatcaaa
     1501 aacccctttg gactcagcgc cgcgcaaacc cgcaccggct ttcccccata ctcgatgtcc
     1561 gacgcacaag cgtcgaaggg tttggactcg gaccgaggct cccccgctgc catacgccag
     1621 tcgtcctcaa acgtggctcg caacgcatcc accacgggcc gcacacgaat caccacaccc
     1681 aattcgagga tatgctccaa ggagcggtaa tcgaagttct gacttccgag ataggcaagc
     1741 tcatcatcca cgacaaaata cttggcgtgc tgaacgccgc ccatgatggc tcccaggtca
     1801 taacgacgca cctcgatctt gggatgtgct tcgagacgct cgggaacctc gtggttatcg
     1861 ccgagcagct tggtatccac aatcatgcgc acgcgaacac cgcgatccgc cgcgcgcaac
     1921 agcgcagcaa tcacccgttc caaggcacta ttcggcgcgc tcttcgcata aaactcggaa
     1981 atatcgatgc gctctcgcgc gctgtcgatg aggtccaccc acgcgacggc ggcatcccga
     2041 atcaacggat catccagact cgtctccgaa ggccacgact cgaccaactc cagggaaggc
     2101 gctttcggca aggaagcagg agcgacaggc gcggcaggag cagccgatgg cggttgaggc
     2161 cggaccgaag gcgccgagca cgcgccaagc atcgatacca aagcggctgc acgccagcac
     2221 caagctctta ccttctgcca tcgccacgta ggccgtgatg tcttgcctga ccgtatacgg
     2281 atagtgtgag aaccgtccag catgcgcgat tcctttcttc ggctcgacac aaccgccgcc
     2341 atgcgaatcg ataggcctac tcctgccgaa atcgcaaatc cgaggcaagc gccaatcgat
     2401 ctctgctcct gtcatcaaga gataccccac ccctcgccgc cgccgtccat gtatcctgac
     2461 ccttggtctt ttttcacctc cgggaagcat tcccggcaag acgaatgctc cccatcgaaa
     2521 gaataacctt caccaaaaga tccaatgaac gccttcccct tcgattggca cacacaatac
     2581 ccttcacgac gttcgcccgt gtttgcccgc aatatcgtgg ccacttccca acccctggcc
     2641 gcgcaagcgg ggcttcgcat gctcgcttcg ggaggcaatg ccgtcgacgc cgccatcgcc
     2701 gctgccgccg cccttaccat cgtcgagcca gtctcgtgcg gattgggagg agatgctttc
     2761 gccatggtgt gggacggtca tcgcctgcat ggcttgaacg cctcgggcac cgctcctgct
     2821 tcatggtccc tgacgtactt cacacgccga tacggtagtg tatttcccca acgtggcgcg
     2881 gatacggtca ccattcccgg cgcggtggcg ggatgggccg ctttgcacaa ccgcttcgga
     2941 cgcctgtcgc tgagcgactg cctcgaacca gccatcgaac tggcttgtca gggtttcggg
     3001 gtcacaccca tcgtgcaaac gaaatgggcc gcgcaagtcg atgcgttgca atcacaaccc
     3061 ggattcgcaa gcaccttcat gcctcacgga cgcgccccct tcgtagggga acgtttcgtt
     3121 ttggcgcagg cagcccaaac gctgcgaaga atcggacaaa gcgcgggacg agatgcttac
     3181 gagggacaaa cagccgagaa aatcgtcggt tttctccgcg aacacggcgg caggatgacc
     3241 ctcgccgacc ttcgcgaatt ccagcccatc tgggtcgaac ccatctcacg cgaataccac
     3301 ggttttgcgc tgcacgagct gcctcccaac ggtcaaggta tcgccgcatt gatcaccctg
     3361 ggcctcctcg atcacttcga cttcccctcg ttggcgcctg acaccgtcac ctttttccac
     3421 ctgcccatcg aagccatgaa ggcagctttt tctcaagtat atcgccatgt taccgatgat
     3481 cggcacatga tcatcgaacc ggtggcgctg ctcgatgacg cccgactcgc ccagcttgct
     3541 tcccgaatcc gactcgatgc cgccaccgac ttcgaccaag gtcttccccc tgctggcgat
     3601 accgtttatc tcgccactgc cgatgccagc ggaatgatgg tttcgttcat tcaatccaat
     3661 tacatgggct tcggttccgg tttggtcgtg ccggatatcg gcgtttcatt acaaaatcgt
     3721 ggatgcggtt tttgtctcga acccaatcac cccaatgtgg tggcccctcg caaacgcccc
     3781 ttccacacca tcatccccgc atttctcacc cgaaacggcg cgccgcaaat gtgcttcggc
     3841 gtcatgggcg gcaatatgca acctcaaggt catgtgcaaa ccctgcttcg catgatcgac
     3901 catcatcaaa atccgcaaac cgcttgcgat gcaccacgat ggaaggtgca gcgcggcctt
     3961 caaatcagcc tcgaaccgtc catccccatg cccgtcagac aaggacttgc cgatctcgga
     4021 catcagatcg ttcacgaaac cgacagctac atggacttcg gaagcggcca attcatctgg
     4081 cgcctgtccg acgatctcgc ggacggttac atggccgcaa gcgaccaccg tcgtgacggt
     4141 tgtgcggtcg ggttttgaaa catattcaac tataccaata cggttagtga atcatttctt
     4201 ccacgagctg cagcaactcc gatcgttcga acggtttggt cacgtatcga tccactccgg
     4261 cttcaaaccc cgcccttcga tccacttcca gatccttgct cgtcaccatc aaaatcggtg
     4321 tgcgatgaaa cgcgggcaac gctcgaagcc gtcgcgtaag ctcatatcca tccatgcgcg
     4381 gcatcaccac atccgtgctg acgagatggt attgccgctg ttgcgccatc atcagccctt
     4441 ccattccatc tcccgcctcg tacacctcaa gccccgcctg ctcgaagaca cgacgcaacg
     4501 caagacgcgt catcgcgtcg tcatccacaa gcagcgcaca acgtttcgac gatgagggca
     4561 cgcttgcgga gggcgcgacg acccgtaccc ccggctttcc gacagagtcg atgatttccg
     4621 cgggatcgac gatgagaaca catcgatctc caagcaatgt cgacccagcc acgaaaggca
     4681 cttgcttgag caacgtcccc aaccctttca caacgacctc tcgctgcccg cgcattctat
     4741 cgagagcaag gcccatgcgc ttgtctccca ggcgcacaac cgtcacataa caactatccg
     4801 gacgcttcga ggcgggtcgc cccatgccca acatctcgtc gagacgcacc aacggcacgg
     4861 tttctcccaa cagagtgaaa atttccgcat ctcccatcgc ttcgatatcc gactcgctca
     4921 gatggaccac acgctccacg gcggacatcg gaatcgccaa ggtatccgtg tcgagttcac
     4981 caagcaccac ggtcgtgatg gcaagcgtca gaggtaacga caactcgatc cgactgcctt
     5041 tgcccatgac cgaactgaca tcgacactgc ccttgatggc gcgaatctta tccatcacct
     5101 catcgagacc caccccacgc cctttcagat cgtcggctcg ctccgcagtg gtgaaacccg
     5161 gacgaaatat gagcccgagc acctcacgct ccgtcatccc ctcgagcgct tctttcgtcg
     5221 caatcccctt ggccaaagcc actttcccca aaaccgccgg atccatgcca cgcccatcat
     5281 ccgtcgtttc gatcacaaac cggcttcctt gttgctgcgc acgaatgcga atcgtccctt
     5341 ccgccacctt tccggctgct tcacgctccg caaccgattc aatcccgtgc gccacggcgt
     5401 tgcgcacgat atgcacgaga gggacctcga gcatgttggc catcattttg tcgatctcgg
     5461 catcggcacc aatcacttcg agcctcgctc gcttcggact cgctcccgca gcgcggttct
     5521 ccttctgcaa cgcatcgaaa accacacggc tcagacgacc aagcaccgta ttcaccggca
     5581 ccatccgcat ctccatgacc tggtcgcgca aatccgatga gatcatgtcc gtctcaccca
     5641 tggcgctgcc aagctgatcg gcagcttgct ccatctccag ttcgagcgcg gctaaccctt
     5701 cctgcaagtg aacggcgtct tcccgcgtaa gccatacccc ttcttccgat gaaccatggg
     5761 cacgacccaa cacgttttga agcgttctca catggaacat cagttccgca tacaactccg
     5821 ccgtatcgtt caaacgaatc ttgttgatga acaaatcccc cactcgattg agagcccgat
     5881 ccaccttctc gaaatccacg cgcaaatact gggctttcga aagcgacgac gaagaagctc
     5941 gagtttcctt gggcgcggaa tccgtaaccg gatgggaagt ggccgacgta tcgaacccta
     6001 ccgaaggcgc tggccccttc gcttccaaca ccatcggcga aacctccgga gaagaagcat
     6061 tcgacgatag ggtcgtcggc acaattaccg gcgatgaccg tcccaccgat cgatcatcgg
     6121 attggctttt cgattcccaa gtcccttgga tgacaccatc cattctctcg gtctctttac
     6181 caatcggatg aacgatgcaa cgcgtttgac gaagctgctc cagcatcgat gccaacgagt
     6241 cgatgaccga aagcaatacc tccacactcg aagccgatag caccagcttg ctttctcgaa
     6301 ccaaggcaag aacgtcttcc agcttatgag ccaccttctc gatcggtcga acactcatct
     6361 ggccgctttg ccctttgatg gagtgcgagg aacgaaacag gtcgttgatc agatcgggct
     6421 gcgagccacc ggaagcgagc tgcacgagcc cttgctggag atgatcgata tgcgccgcga
     6481 cttcctccac gtagtcgctc tccatatccg gatagggagg atcccacggg ccccaggact
     6541 cggactccgc ttccgatcga tctatattat tgatcttatt gatattttta tgattctgaa
     6601 ggtctgaggc cgctgtttcg gaaagctcgg cagacgccgg catgaggcaa gacgtcatgg
     6661 atgtgatctt gttcgcgcat gaccgcagcg cgtccacatt cagcccatcc cccgcctcga
     6721 gtgataaaaa cgcctcttcg agcacgccaa cggcctgcac gaaaaaacct cccgccgtcc
     6781 caaaaggatc gaaatcgccc tgcgccagcc ccgacaccac cggaaccatc gccatggcaa
     6841 catccgcgag ggattcgaga ctctccgccc gcgcggaaat cccaagca
//
LOCUS       DAOSOR010000303         6888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_11071_length_6888_cov_4.087224, whole genome shotgun sequence.
ACCESSION   DAOSOR010000303 DAOSOR010000000
VERSION     DAOSOR010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11071_length_6888_cov_4.087224"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PK710_15415"
     CDS             <1..192
                     /locus_tag="PK710_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11168.1"
                     /translation="VRFAKTLLAKVSAMLPDPRTSPAHDALAGAIITSGSIEAPVRER
                     LAWLLDLTSKSSVDPGHQE"
     gene            197..1123
                     /locus_tag="PK710_15420"
     CDS             197..1123
                     /locus_tag="PK710_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOT11169.1"
                     /translation="MNEKAVLIVGSMALDDLELPSTTAKNVLGGAATYAAFAASLFAP
                     ARIVAVVGEDFSQNDIETLMKHGIDTSGVERVKGKTFRWAGRYGANLTSRVTLDTQLN
                     VFAGFQPKLPAAYRSSDYVLLANIHPALQLQVLDQVTQPKLVLADTMNFWIEGEPALL
                     NKVLERVDVLCINDEEARLLSGVYPLLQAAQIILGKGPKSLIIKRGENGAVLVDSKGM
                     FWVPAVPLNDAVDPTGAGDSFAGALVGYLASEDELTPESLRRALYVGTAVASFNVQAV
                     GTARLARLTIQDVERRIAELTAMTHLPAQTHG"
     gene            complement(1126..2055)
                     /locus_tag="PK710_15425"
     CDS             complement(1126..2055)
                     /locus_tag="PK710_15425"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11170.1"
                     /translation="MPNPHLLARHGAILAVRDDLSALRVSGPDRFSWLQALLTCDVNP
                     VKNGQAVYGLVLHRNGKILADVFLIARDSDVLLVTDKLDADALLAHLDAYLVMEDADI
                     EVLEPPITFVLAIGPQAPAIASAASNHPGIHASGHGTLLATSAALLGGAPNEIQHILP
                     SLLRPDDSSMLVSNDDFDALRVDLGFPKWHKDFDGANYPMEIGLDQVAISFQKGCYVG
                     QEVVVKLRSRGKPVRLLIRLALDSNTLPDVGSPVSSLSGKEIGTITNVGYSMQKRALA
                     FALVRRSEVEASPTVVVQGIQAHIDALWKPFEG"
     gene            complement(2099..2425)
                     /locus_tag="PK710_15430"
     CDS             complement(2099..2425)
                     /locus_tag="PK710_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOT11171.1"
                     /translation="MAEKKNAPKRMSKAQIISELAEASQVDKKAVNAVLASLVDLVGK
                     ELGAKGPGEFVLPGLVKLKATKKAATKDRQGINPFTKQPMLIKGKPASKKIRVTAVKA
                     LKDIVQ"
     gene            complement(2562..5081)
                     /locus_tag="PK710_15435"
     CDS             complement(2562..5081)
                     /locus_tag="PK710_15435"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOT11172.1"
                     /translation="MANQQSDQNAGTKIRVEDDRTGMDPIVLERAFTDHLQYSQGKSL
                     DSATPHDEFMSLAFLVRDRLVRRWIQTHKTYHEKDVKRVYYLSAEFLLGRALASNLIS
                     LGIYDDCQRMLADRGISLADLLEQEPDAGLGNGGLGRLAACFLDSMATLGLPSIGYGI
                     RYQFGIFEQVLRNGYQVERPEEWLKYGNPWEIKRPERQVRVGFYGRAEHYYDQAGQWR
                     ARWVDTRNVIGMPYDTPIAGFGNETVNTLRLWEAVASEEFDLDVFNDGDYERAVADKN
                     ASEIISKVLYPNDKTVLGKELRLKQQYFFVRCALVDILRRHRVNHPSLANLTDTAAIQ
                     LNDTHPAIAVAELMRILVDEHAMDWGEAWKITKNTIAYTNHTVLAEALETWSIDLIGR
                     LLPRHLQIIFEINRRFLRDVLIRWPNDLSRLTRMSLVAEGEEKRVRMAHLAIVGSHSV
                     NGVAKLHTEILQRRVFKDFFEMEPQQFSAKTNGISPRRWLLQCNPALSNAISQRIGTG
                     WTRDLDQLEKLVPFADDPEFQKQIRTIKRKNKLHLATITKGLTGMSPDPDALFDMQIK
                     RMHEYKRQLLNVLHIVALYLRAKNSPNALSVPRAFFFGGKAAPGYHAAKLIIKLINAV
                     ADTIAADYHVRGLSVHFMPNYRVSLAERMIPACDLSEQISTAGMEASGTGNMKLALNG
                     ALTIGTLDGANVEIREAVGPDHFFLFGWTAEQVEQSIDGYVPEDMVKSNPELKQVLDL
                     IASGFFCPENRGLFLPLIDSLMVSDPYRILADFQSYLECQLRVEQAYLDSARWTRSCI
                     LNIAKMGRFSSDRTVRDYATDIWNLSPVQVKVPRDISSDPT"
     gene            5210..>6888
                     /locus_tag="PK710_15440"
     CDS             5210..>6888
                     /locus_tag="PK710_15440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT11173.1"
                     /translation="MSLYALLQIGAIGSNLFVLGLAVGRWRVAALHWAALLSIIAPLG
                     WATTTLFSLLSISQGHETTLVRVAALFWIPLAYWVLSFLYRLLDRKRDTLYYLLGVLT
                     AAALFLYVGTDWVVQGATRHAWGIAVQRTPVHVAITSYVGALGLLALGFMALAWKQAT
                     SRTRARSLAILTIGCSLAFVFVFTTDVIFPLSFHPNPFPELGSYGVVLISPILYWIVA
                     RYDYLRLEPRQVAEQLFESAQDGILLVDASGVIQHANQASRRMVASAKLVGQPVEELI
                     RIVESQKPNVKSSTGGPLYRHIDVTRSKTAHPEIGELVMLRDITEQRLAQDVLRKSHE
                     ELKQQVQQRTEELDHAQKMEAIGTIAGGIAHDFNNILAAVVGFAMAAKSDLPQTHRVQ
                     KDLDDILMASNRGRGVVQQLMSMTRRRKAYRSLIDGASCVAEAVQLARIMAPKQVSVH
                     FNPVATHTVRADSAQITQVLLNMASNAFQAMNPTGGTLTIETDVTRTRLALELNDREQ
                     LPAGQYFQITVSDSGPGMLPEVASHVFDPFFTTKNEEGTGLGLATAHRIVK"
BASE COUNT         1434 a         1740 c         2144 g         1570 t
ORIGIN      
        1 gtacgattcg cgaaaacgtt gttggccaag gtatctgcaa tgttgccgga tcctcggacc
       61 agccccgcgc acgacgctct tgcgggagcg atcattacat ccggatccat cgaggctccc
      121 gtgcgtgagc ggctagcctg gctgctcgat ctcaccagca agtcgtccgt cgacccaggg
      181 catcaggagt aaggtcatga acgaaaaagc tgttctcatc gtaggttcca tggcgttgga
      241 cgacctggag ttgccgagca cgacagccaa aaatgtgttg ggtggtgcgg ctacttatgc
      301 ggcttttgcg gcatcgctat tcgctcccgc ccggattgtc gcggtggtgg gggaagattt
      361 ttcgcaaaac gacatcgaga cgttgatgaa gcacgggatc gacacctcgg gcgtggaacg
      421 ggtcaaaggc aagacgtttc gttgggcggg tcgttatggg gccaatctga cgagtcgggt
      481 cacactcgac acgcagctca atgtttttgc gggtttccag cccaagttgc cggcggcgta
      541 tcggtccagc gactacgtgc tgctcgcgaa tattcatcct gctttgcagc ttcaggttct
      601 cgatcaggtg actcagccca agctggtgtt ggccgatacg atgaatttct ggattgaggg
      661 cgaaccggcg cttttgaaca aggtattgga acgggtagac gtgctttgca tcaatgatga
      721 ggaagcgcga ttgttatcgg gcgtatatcc cctgcttcaa gcggcgcaaa tcatcctggg
      781 caaaggtccg aaaagcctca tcatcaagcg gggagaaaac ggcgcggtat tggtggacag
      841 caaaggaatg ttctgggtgc cggcggtgcc gttgaatgat gcggtggacc caacaggagc
      901 aggggattcg tttgccggcg cgttggtagg ctatctggcc tctgaggacg aattgacccc
      961 ggaatctctg cgcagagcgc tgtacgtggg cacggcggtg gcatctttca acgtgcaggc
     1021 cgtaggcaca gcccggctcg ccagattgac gatacaagat gtcgagcgtc ggatcgccga
     1081 gcttacggcg atgacgcatc ttcccgcaca aacccacggt tgatttcaac cttcgaaagg
     1141 tttccacaga gcgtcgatat gcgcttggat tccttgcacc accacggtcg gtgatgcctc
     1201 gacctcggaa cgtcgcacca aagcaaacgc aagagcgcgt ttttgcatgg aatagccgac
     1261 gttagtgatg gttccgattt cttttcccga taacgacgat acaggacttc cgacatcggg
     1321 aagggtgttt gaatcgaggg cgaggcgaat cagaagacgg acgggttttc cgcgtgatcg
     1381 tagtttgacg actacctctt ggccgacgta gcagcctttt tgaaaggaga tggccacctg
     1441 atcgaggccg atttccatgg ggtagttggc accatcgaag tctttatgcc atttgggaaa
     1501 tccgagatcg acacgaagag catcgaaatc atcgttcgac acgagcatgg aagaatcgtc
     1561 cggccgtagc agcgatggta ggatatgctg gatttcattg ggtgcgccgc caaggagcgc
     1621 ggcggaagtt gccagcagcg ttccatggcc tgaagcatga atgccgggat gattcgaagc
     1681 ggctgaagcg atggcgggag cttggggacc gatggcgaga acgaacgtga tgggtggttc
     1741 gagcacttcg atatcggcat cttccatcac gagatacgcg tcgagatgag ccagcagcgc
     1801 gtcggcgtcg agcttgtcgg tcacgagcaa aacgtcggaa tcccgggcga tgagaaagac
     1861 atcggccagg attttgccat ttcggtgcag aacgaggccg tagacggctt gtccgttttt
     1921 gacggggttg acatcacagg tcaaaagggc ttggagccac gagaagcggt cggggcccga
     1981 tacacgaagg gcggatagat catcacggac ggcgaggata gcgccgtggc gagcgagaag
     2041 atggggattg ggcatgaccg gtgcttgccg cttccggcca tcgggcgcaa ggggcaggct
     2101 attggactat gtctttgagg gccttgacgg cggtgacacg gatttttttg gaggcgggct
     2161 tgcctttgat gagcatgggc tgcttggtga aagggttgat gccctgacga tctttggtgg
     2221 ccgctttttt tgttgccttg agcttgacga gaccgggaag gacgaattcc cctggtcctt
     2281 tggcaccgag ttctttgcct accaagtcga ccaagctggc gagtacggca ttcaccgctt
     2341 ttttatcaac ttgcgatgcc tcggccagtt cggaaatgat ttgcgctttg gacatgcgct
     2401 tgggagcgtt ttttttctcg gccatcgttg tctctccatg gttcgacgag ctgatgcgcg
     2461 tcaaagggtt gattgtcatg cccgccaacg gacggacgga gtgccatggg tgacacagtt
     2521 ttgggcagat atgcaaggag tttaggtata accgggctta attacgtcgg atcggagctg
     2581 atatcccggg gaaccttgac ttggacgggg gataggttcc agatgtcggt ggcgtagtcc
     2641 cggacggtcc gatcggagga aaagcgtccc attttcgcta tgttaaggat acaggagcga
     2701 gtccaacggg cggaatccag gtaggcctgt tcgacacgaa gttggcattc caggtagctc
     2761 tggaaatccg caaggatacg atacgggtcg gagaccatca gcgagtcgat gagggggagg
     2821 aacaagccgc ggttctcggg gcagaagaat ccggaagcga tgaggtcgag cacttgtttc
     2881 aattcggggt tggatttgac catatcctca gggacgtaac catcgatgga ttgttcgact
     2941 tgttcggcgg tccagccgaa gagaaagaag tggtctgggc cgacggcctc gcggatttcg
     3001 acattggcgc catcgagggt gccgatggtg agagcgccgt tgagggcaag tttcatgttg
     3061 ccggtgccgg aggcttccat gcctgcggtc gagatttgtt cggacagatc gcaagcggga
     3121 atcatgcgtt cagcgaggga aacgcggtag ttgggcatga aatgaacgga cagtccacgg
     3181 acgtgataat cggcggcgat ggtatcggcg acggcgttga tgagtttgat gatgagtttg
     3241 gcggcgtggt agccgggagc agcttttccg ccgaagaaaa aggctcgggg tacggacagg
     3301 gcattggggc tatttttcgc gcgaagatag agcgcgacga tgtgcagcac gttgagaagc
     3361 tgacgtttgt actcgtgcat gcgtttgatt tgcatgtcaa aaagggcgtc gggatcgggg
     3421 gacatgccgg tgaggccctt ggtgatggtg gcgaggtgaa gcttgttctt tcgtttgatg
     3481 gtgcgaattt gtttttgaaa ttccgggtca tcggcgaagg gcacgagttt ttccagttgg
     3541 tcgaggtcgc gagtccatcc ggtaccgatg cgttgggaga tcgcgttgga cagggcgggg
     3601 ttgcattgca ggagccagcg acgcggggag atcccgttgg tcttggcgga aaactgttgc
     3661 ggttccatct cgaaaaagtc cttgaagact cgtcgttgaa ggatttcggt atgaagcttg
     3721 gccactccat tgaccgaatg agaaccaacg atggcgagat gggccatgcg aacgcgtttt
     3781 tcctcgcctt cggccaccag agacatgcgc gtaaggcgag acaaatcgtt gggccaacgg
     3841 atcaaaacgt cgcgtaagaa gcgacgattg atttcgaaga tgatttgcag atgtcggggg
     3901 agcagccgac caatcaggtc gatggaccat gtttccaggg cttccgccag cacggtgtga
     3961 tttgtataag ctattgtatt ttttgtaatt ttccacgcct ccccccaatc catcgcatgt
     4021 tcatcgacga ggatacgcat cagttcggcg accgcgatgg ctgggtgggt atcgttgagt
     4081 tggatagcgg cggtatcggt caagttggcg aggctgggat ggttgacgcg gtgtcgtcgg
     4141 aggatatcga cgagggcgca tcgaacgaaa aagtactgtt gtttcaggcg cagttctttt
     4201 ccgaggacgg ttttgtcatt ggggtaaagg actttggaga tgatttcgga agcattttta
     4261 tcggcgacgg cgcgttcgta atcgccgtcg ttgaacacat cgagatcgaa ttcttcgcta
     4321 gcgacagctt cccacaggcg cagggtgttg acggtttcgt tgccgaagcc agcgatgggt
     4381 gtgtcataag gcattccgat gacgtttcga gtatcgaccc atcgagcacg ccattgaccg
     4441 gcctggtcgt agtagtgttc ggcgcggccg taaaagccga cgcgcacttg tcgttcgggg
     4501 cgtttgattt cccaagggtt accgtatttc agccattctt ccgggcgttc cacttggtaa
     4561 ccgttgcgaa gcacttgctc gaagataccg aattgataac ggattccata gccgatgctg
     4621 ggcaagccga gcgtggccat cgagtcgagg aaacaagcgg cgagacgacc gaggccaccg
     4681 ttaccgagac cggcgtcggg ctcctgttcg agcaagtccg ccagcgatat gccccgatcc
     4741 gcaagcattc gctgacaatc gtcatagatg cccaggctga tgagattgct ggcaagcgcg
     4801 cgtccaagca aaaattcggc ggacaggtaa tatacgcgct tcacatcttt ttcatgatag
     4861 gttttgtgcg tctgtatcca gcgtcgcacg agacgatctc gaaccaggaa cgcgagactc
     4921 atgaactcgt cgtgaggcgt tgcggagtcg agacttttgc cttgcgagta ctggaggtgg
     4981 tccgtgaagg cgcgttcgag cacgatggga tccatccccg ttcggtcgtc ttccactcgg
     5041 atcttcgttc ccgcgttttg gtccgactgc tgattcgcca ttccgatctc cttccgcggt
     5101 tgacgtgact tgcacgcgta ctacggtggc cgcggttgta cggcaagagc ttccgctcac
     5161 tccgagacga taccatgccg cgcggtggtg cggcgacgca cgttttgcca tgagccttta
     5221 cgcattgctt cagataggcg cgatcggaag caacctcttt gtgttggggc tggcggtggg
     5281 tcggtggcgg gtggcggcgc tgcattgggc ggcgctatta tccatcatcg ctccgttggg
     5341 atgggcaacg accacgttat tttcgttgtt atccatttcc caaggccatg aaaccaccct
     5401 ggttcgtgtg gcggccctat tctggatccc tttggcttat tgggtcctga gtttcctgta
     5461 ccggttgctc gatcgaaagc gcgatacact gtattacctg ttgggggtgc tcaccgccgc
     5521 agcgctattt ctttacgtgg gcaccgattg ggttgttcaa ggagcgactc ggcacgcctg
     5581 gggaattgcc gtccaacgaa ctccagtcca tgtggccatc acgtcgtacg tcggagcgct
     5641 tggcttgcta gcccttggat tcatggcctt ggcctggaaa caagctactt cgcgcacacg
     5701 cgcccgctcg ctggccattc tgaccatcgg ctgcagtctc gctttcgtat tcgtttttac
     5761 caccgacgtc atttttccgc tttcgtttca ccccaacccc tttccggaac tcgggtctta
     5821 tggcgtcgtg ctcatcagcc ccatcctgta ttggattgtc gcccggtacg attacttacg
     5881 cttggaaccg cgccaagtgg ccgaacagct tttcgaatcc gcgcaagacg gcatcctgct
     5941 ggtcgacgcg agcggcgtga tccaacatgc caatcaagcg tcccgtcgga tggtggcttc
     6001 tgccaagctc gtcggccagc cggtggaaga actcatccgc atcgtggaat cccagaagcc
     6061 gaacgtcaaa tcctccaccg gcggcccgtt ataccgacat attgatgtga ctcggagcaa
     6121 aacggctcat ccggagattg gcgaactcgt catgcttcgc gacatcaccg aacagcgtct
     6181 tgcccaagac gtattgcgca aatcccatga agaattgaag cagcaagtgc agcaacgcac
     6241 cgaggagttg gatcacgcgc aaaagatgga agccattgga acgatcgcag ggggaatcgc
     6301 gcacgacttc aacaacatcc ttgcggccgt ggtagggttt gccatggcgg ccaaatccga
     6361 cctaccgcaa actcatcgcg tgcaaaaaga tctggacgat atcttgatgg cctccaatcg
     6421 cggccgaggc gtcgtgcaac aactcatgtc gatgacacga cgtcgcaagg cctatcgatc
     6481 gctgatcgac ggagcatctt gcgtggcgga ggccgtgcag ctcgcccgga tcatggcgcc
     6541 aaaacaggtc agcgttcact tcaatcccgt ggccacccat acggttcgag ccgattccgc
     6601 gcaaatcacg caagtccttc tgaacatggc gtccaacgcg tttcaagcga tgaatcccac
     6661 ggggggcacg ctgacgatcg aaacggatgt gacgagaacc cggttggctc ttgaattgaa
     6721 tgatcgggag cagcttccgg cgggacaata ttttcagatc acggtaagtg attcgggacc
     6781 gggtatgctt ccggaagtag ccagtcacgt tttcgatcca tttttcacta cgaaaaacga
     6841 ggaaggaacg gggcttggcc tggccaccgc ccatcgtatc gtgaagga
//
LOCUS       DAOSOR010000304         6873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_11104_length_6873_cov_4.486213, whole genome shotgun sequence.
ACCESSION   DAOSOR010000304 DAOSOR010000000
VERSION     DAOSOR010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6873
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11104_length_6873_cov_4.486213"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            308..4615
                     /locus_tag="PK710_15445"
     CDS             308..4615
                     /locus_tag="PK710_15445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOT11174.1"
                     /translation="MVEARIDDASLVLGVDVGSVAVSLACVDRQGKVACEGYALHRGD
                     VSGTLRQLLVGLDWTRIRAVGATSRAPAVVVAQQPYDSVVAYIHAARFVYGSMNALLV
                     VGGETFTLVRFGADGSYQESRGSSSCAAGTGSFLDQQARRLGLEGSAALSRTAFLANG
                     SMPTIASRCSVFAKTDLIHAQQQGYSLEAICNGLCHGLAANVVDTLFGGRDIPAGPIV
                     FAGGVSRNEAVGRHLEKMIDRRLQVDGRSHLFGAMGAALLMDADVRSGRVEPVVLRGM
                     EDLMRSASVQKRYIHPPLELRRSSYPDFSQTRQLCLQPRGIHGAAAVEVDVFKPLRKG
                     ESCRAWLGIDIGSTSTKAVLLGAKDEAPYGQEREISDRVIAGFYTRTAGQPLQATQAL
                     FEVIDTLMREHGVALQIAGVATTGSGRKLIGAIVGADEIMDEITAHAHAAHALDPRVD
                     TIIEIGGQDAKFTTLAQGRVTFSVMNNVCAAGTGSFIEEQANRLGCQLASCARRTQGQ
                     RAPLTSDCCTVFMERDINHYLASGYEVDEVLAAALHAVCENYLRKVARESAIGHRIHF
                     QGATAKNRSLVAAFEQRLGKPIHVFRYCHLTGALGAALLIEEQPPKETIFRGLELWKE
                     TIPMQEQDCGLCNNHCKLKLATVGNECVAYGMLCGRDYETERYVSVNRSGFQLLKAWR
                     KHWQVPATKRRWPITIGIPASITLHEDLPLWRNFFANLGVSTVSSEGFKDAVRIGKRL
                     AGAEFCAPMAAYHGHVAKLAHEADYVFSPHYLEDDDRPVGTRRNFCYYSQYAATLAAT
                     IPDPLVRSKLLTPVLKNGERERTRIRRLVHTLRPVLGATLRWGEVASAYRDALKTVRE
                     RKASLPRIMQEQRDKAPNELAVVLLGRPYTVLSASMNKNIPELFGALGVRCFSQSMVE
                     VTEAHLQSIEPLLSLIHWAYPAQTLAAAEAVASTPGLYPVIVTSFKCAPDSCAIEYVR
                     RILDRRGKPYLVLQLDEHDSQVGYETRIEAALRTFRNHHASASESKRSPSPHRAVVQE
                     PLYDLRDRVLLLPCWDRYACTMMAASFRREGVDARVLEEDSLLITQALQHNSGQCIPL
                     NVMVEGFVTYVKKHGLDPSRAALWTHSSQMGCNIGAFPLVMQSLLEERGEGFEHARVY
                     VGQMTGKDISMRAIANQVRVFVCAGNLRRMGCKIRPYELEPGQTDRLLEDSLVRLARA
                     FETGETLLPVVEEVVSRFSAIRRRDEKRPKVAIFGDLYVRDNDVFNQGLIATLEAQGA
                     EVVTTPYMEYTKLIAAAYLRKWVREGKVAQAVQGRLMLLAAQALERDLVRQFERVLGP
                     EPRVSKMKMEKVLAPYRVTVQHTGESFDNLLKIHHLMHVYPDIELFVQLSPAYCCPSL
                     ITEAMAAQIRQRTGVPVVTITYDGTRVPKNGVVAPYLHFLRNGNHGTRLVGKPIRGS"
     gene            complement(4819..5355)
                     /locus_tag="PK710_15450"
     CDS             complement(4819..5355)
                     /locus_tag="PK710_15450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOT11175.1"
                     /translation="MKTAVVCLGNELVGDDGVGMRVARVLDQLHLPDNIDVVVRPFLG
                     LDLVDLLHQYERLVIVDAMTSGRPAGTCVCLDPSEAKAMASHPSCAHGLGIAEVMQLV
                     DKLYPDRKGRVIGFVGVEAAALQQFEVGLSEPVACALPKAVEAVLLMVGGLEAWWEEA
                     RRLAEIESRTTMTLEQIL"
     gene            complement(5497..6684)
                     /locus_tag="PK710_15455"
     CDS             complement(5497..6684)
                     /locus_tag="PK710_15455"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6348 family protein"
                     /protein_id="HOT11176.1"
                     /translation="MSPSPPSVTAAVRVVVLNWNAAWLTARCVRSLQATDYPAERLEI
                     VVVDNASIDGSLARLRADLPGVTFVANEHNYGFAEGNNRALRDLAGVDYVALINNDAV
                     VSPGWLRPLVAALDAEPDVGAAAPKMLLESEFATVRLRGCGTLTGIEVDGVERTVTWS
                     ISSVPLSQTETSRPWLQAFEATLSRSTIPTGLHWVRVTHIQLDRNPIVWEVLLDNKPW
                     EFGQESLHDAPWPSRDGYASERLFAMLQGGVDVSRAVARMIEAAGAPDKDIQPVLMAD
                     GATAREAQALLAFVPLAFGRMVLQSLPVHFSETALVRNDRGQQGTEILLSDDPIFARA
                     QDLAADAWQATTFTQDEFLAVAMRSAEVHAVNQAIEQGAQACDLIVSPPTITLSPDDT
                     ASP"
BASE COUNT         1354 a         1855 c         2228 g         1436 t
ORIGIN      
        1 ttccgctacg tggattccgt gggggccagc gaaattttgc gtcgaatcga gggatatcac
       61 gatcgattcg gcgcgcgttg ggagcctgct ccggttttga tcgagatggc caagaacaat
      121 aaaaaattcc acgaatgacc cccacacgct gatcggaagg accaggtgcc tttcgcttga
      181 accgctcaac gctcgccttt cgcgtgtgat gggcctctat tctcgtcagt tcccctgcga
      241 aatcctgtgg caaaccctgt ccattacggg caaacagggg gtgtgacgat cggttgtagg
      301 actcgttatg gttgaggcaa gaatcgacga cgcgtccctt gtgcttgggg tcgacgtagg
      361 ttccgtggcg gtttccctcg cctgcgtgga tcggcaggga aaggtggctt gcgaagggta
      421 tgccctgcat cgtggcgatg tttcgggaac cttgcgacaa ctgttggtcg ggcttgactg
      481 gacgcgaatt cgcgcggtgg gcgccacgtc tcgggcgccg gccgtggttg tggcgcagca
      541 gccgtacgac tccgtcgttg cgtatattca cgcggcgagg ttcgtgtacg gctcgatgaa
      601 cgcgttgctc gtggtggggg gagaaacgtt tacgttggtt cgttttggcg cggatggttc
      661 gtatcaggag agtcgcggca gcagttcttg cgctgcggga acggggagtt ttctcgatca
      721 gcaagcgcga aggttggggc tcgagggcag cgctgccctc agtcgtaccg cgtttttggc
      781 caatggatcg atgcccacga ttgcgtcgcg ttgttctgtc ttcgcgaaga cggatttgat
      841 tcacgcgcaa cagcaagggt atagcctcga ggcgatctgc aatggattgt gtcacggtct
      901 tgcggccaac gtcgtggaca cgctctttgg ggggcgcgac attcccgccg ggcccatcgt
      961 atttgccggc ggtgtcagtc gcaacgaggc ggtggggcgc catttggaga agatgatcga
     1021 tcggcgcttg caggtggatg gtcgttcgca tcttttcggc gcgatgggtg cggcgttgct
     1081 catggatgcc gatgtgcgat cgggtcgtgt tgagcctgtt gtccttcggg ggatggagga
     1141 cctcatgcgg tcggcttccg tacaaaaacg ttatattcat ccaccgctcg agctgcggcg
     1201 atcttcttat ccggactttt cgcaaacgcg acagctatgt ctgcaaccgc gcggcattca
     1261 cggcgcggct gctgtcgaag tggatgtctt caagcctctt cgaaagggcg aatcttgccg
     1321 tgcctggctc gggatcgaca ttggctcgac gagtaccaag gccgtattgc ttggtgcgaa
     1381 ggatgaagcg ccatacgggc aagagcgtga gatttccgat cgcgtgatcg cggggtttta
     1441 tacgagaacg gcggggcagc ccttgcaagc gacccaggcg ttgttcgagg tgatcgatac
     1501 gctgatgcgt gaacatggcg tggcgttgca gatcgcgggt gtggccacca cggggtcggg
     1561 tcgaaaattg attggcgcga tcgtgggtgc cgacgaaatc atggacgaga tcaccgccca
     1621 tgcccatgcc gcccatgcgt tggatccgcg agtggatacg atcatcgaga ttggtggtca
     1681 ggacgcgaag ttcacgaccc ttgcccaggg acgggtgacg ttttccgtca tgaataacgt
     1741 atgcgcggcg ggcacgggga gtttcatcga ggagcaagca aaccggttgg gctgccaact
     1801 cgcgtcgtgc gcgcggcgaa cccagggtca gagagcgccg ctaacgagcg attgttgcac
     1861 ggtattcatg gagcgggaca tcaaccatta cctggcttcg ggctatgagg tcgatgaggt
     1921 attggcggcg gcgctgcacg cggtatgtga gaactatctt cggaaggtgg ctcgggaatc
     1981 cgccataggc catcggatcc attttcaagg agcgacggca aaaaaccggt ctttggtggc
     2041 ggctttcgaa caacgtcttg gaaaacccat ccatgttttc cggtattgtc atttgaccgg
     2101 agcgcttggg gcggcgctgt tgatcgaaga acaaccgccc aaggagacga tatttcgcgg
     2161 gctcgaactg tggaaagaga cgatcccgat gcaagagcaa gactgtgggt tgtgcaacaa
     2221 ccactgcaag ctcaagctgg ccacggtcgg aaacgagtgc gttgcctacg ggatgctttg
     2281 cggacgagat tacgaaacgg agcgatatgt atccgtcaat cgaagcgggt ttcagttgct
     2341 caaggcatgg cgaaagcatt ggcaggtgcc agcgaccaag agacgatggc ctatcacgat
     2401 cgggataccc gcgagtatca cgcttcacga agatcttccg ttatggcgga attttttcgc
     2461 taatctaggt gtttctacgg tgagcagcga agggttcaaa gacgctgtcc gtatagggaa
     2521 acgtcttgcg ggagcggagt tctgtgcgcc gatggcagcg tatcatggac atgtggccaa
     2581 gcttgcccac gaggcggact acgtgttcag tccgcattat ttggaagacg atgatcggcc
     2641 cgtgggaacg cgcagaaatt tttgttatta cagtcagtat gcggcaaccc ttgcggctac
     2701 gattcccgac ccgttggtgc gtagcaagct tctgactccc gtgttgaaaa acggtgagcg
     2761 tgagagaaca cgaatccgac ggctcgtgca tacgctacgg cccgtgcttg gtgccacgtt
     2821 gcgatggggc gaggtcgcta gcgcgtatcg tgatgcgctg aaaaccgtgc gagagcgaaa
     2881 agcgtcgcta ccccgcatca tgcaggagca gcgggacaaa gcgccgaatg agcttgcggt
     2941 ggtgttgcta gggcgaccgt atacggtgct ttcggcgtcg atgaacaaga atattcccga
     3001 gctatttggg gcgctggggg ttcgttgttt ttcgcagtcg atggtggagg tcaccgaggc
     3061 tcatctgcaa tcgatcgaac ccctgctctc gttgatccat tgggcgtatc cagcacaaac
     3121 gctcgcggcc gcggaagcag ttgcctcgac accagggctt tatcccgtga tcgtcacatc
     3181 cttcaaatgt gctccggata gctgcgccat cgaatatgtg cgtcgcatcc tagatcgtcg
     3241 gggcaaaccc tatttggtac ttcaactcga cgagcacgat tcgcaggtgg gatacgagac
     3301 gcggatcgaa gcggccctca ggacatttcg caatcatcac gcttctgctt cggagtccaa
     3361 acgctcgcca tcgccacacc gagccgtggt gcaagagccc ttgtatgact tgcgggatcg
     3421 cgttttgttg ttgccctgtt gggatcgcta tgcctgcacc atgatggcgg caagctttcg
     3481 tcgcgaaggg gtcgatgccc gtgtgctcga agaggattcg ctgctcatca cgcaagcgtt
     3541 gcaacacaac agcggtcaat gcattccgct gaacgtgatg gtggaaggct tcgtgaccta
     3601 tgtcaaaaag catgggctcg atccttcgag agcggcgttg tggacgcata gttctcagat
     3661 ggggtgcaat atcggcgcgt ttccgctcgt catgcagagt cttctggagg aaaggggcga
     3721 aggcttcgag cacgcgcgcg tgtatgtcgg acagatgacg ggcaaggata tatcgatgcg
     3781 ggccatcgcc aatcaggtcc gggtgtttgt atgcgcgggg aatcttcggc gcatgggctg
     3841 caaaatccgt ccgtacgagc tggagccagg gcaaaccgat cggttgctgg aggatagtct
     3901 ggttcggttg gcgcgggcat tcgagacggg cgagacgtta ttgccggtgg tggaggaggt
     3961 ggtttcgcgg ttttcggcga ttcgccgccg ggacgaaaaa cgtcctaagg tagcgatatt
     4021 tggggattta tacgtacgcg acaacgatgt gttcaaccag gggttgatcg caacgctcga
     4081 ggcgcaaggc gcggaggtgg tgacgacccc ttatatggaa tacacgaagc tgatcgcggc
     4141 tgcgtatttg cgaaaatggg tgcgggaggg gaaggtagcc caggcggttc aaggcaggtt
     4201 gatgctgctg gcggcacagg ccctcgagcg ggacttggtc cggcaattcg agcgggtgtt
     4261 ggggcctgag cctcgcgttt cgaagatgaa aatggaaaag gtgcttgcgc cctatcgggt
     4321 gaccgtgcag cacacggggg agagcttcga caacctgctg aaaatacacc atttgatgca
     4381 tgtttatccg gatatcgagc tgttcgtgca gctcagcccg gcgtattgtt gtccgtcgct
     4441 catcaccgag gcgatggccg cgcagatacg tcagcggacg ggagtgccgg tggtgacgat
     4501 tacctatgat ggcacgcgag ttcccaaaaa cggggtggta gcgccgtatt tgcatttttt
     4561 gcgaaacgga aaccatggga cacggttggt gggcaagccg atacggggct catgaccgac
     4621 ggttcgagtt gctcgaggtt tgacgcggcc tttgtatctc gcggatttta tgcgggtttc
     4681 gggacaccgc ggccttctcc aaaccttcct ctggctccgc tgtcaaaatc gattcggacg
     4741 tctgttccac cggctcttgc tcatggggat tccttcgcgg ccccggaaat ccgaaacagt
     4801 aataacctat tgaatatatt ataaaatctg ctcgagcgtc attgtggttc gagactcgat
     4861 ttcagccagc cgtcttgcct cctcccacca cgcttcgagc ccacctacca taagcagcac
     4921 cgcctccacc gccttgggca acgcgcacgc cacgggctcg ctcaatccaa cctcgaactg
     4981 ctggagggcg gcagcctcga cccccacgaa accgatcaca cggcctttgc gatccggata
     5041 cagcttgtcg acgagctgca tgacctccgc gatacccagg ccgtgcgcac acgacgggtg
     5101 cgacgccatg gctttcgcct ccgatggatc gaggcaaacg caagttcccg cggggcggcc
     5161 actcgtcatg gcgtcgacga tcaccagccg ctcgtattga tgaagcaaat caacgaggtc
     5221 caatccaaga aacggccgga ccacgacatc gatgttgtct ggaaggtgaa gttggtcaag
     5281 gacgcgagcg actcgcatgc ccactccgtc gtcaccgacc aactcattgc cgaggcagac
     5341 gacagccgtc ttcacgctac gcctcctgcc tgaacgagta tcgaaacgca ctgtaaaagc
     5401 agacctttgc gcgttgggta gctagccgcg acgggctgga ttggttcgac ccatggcgat
     5461 gtactgcacg atggagaagc aagtgcgatg ttcatgtcac ggcgacgcag tatcatcagg
     5521 gctcagggtg atggtggggg gagaaacgat caggtcgcaa gcctgcgcac cttgttcgat
     5581 ggcttgattg accgcgtgga cttcggcgct acgcatcgcg accgcaagga attcgtcttg
     5641 tgtaaaggtg gttgcctgcc aggcatcggc ggcaaggtcc tgcgcgcgcg cgaaaatggg
     5701 atcgtcggat agaagaattt ccgtgccttg ctgcccgcga tcgtttcgaa ccagggccgt
     5761 ttccgaaaaa tgaacgggca aggattgcag caccatgcga ccgaacgcca gcgggacgaa
     5821 ggcgagcaac gcttgcgctt cccgagcggt ggccccatcg gccatcaaca cgggctgaat
     5881 gtccttatcc ggtgcgcccg cggcctcgat cattcgagcc acggcgcggc ttacgtccac
     5941 gcctccttgc agcatggcaa ataggcgctc gctggcgtat ccgtcccgcg aaggccatgg
     6001 cgcgtcgtgc aaagactcct ggccgaattc ccagggcttg ttatccaaca acacttccca
     6061 cacgatcggg ttccgatcga gttgtatgtg ggtcacgcgc acccaatgca gaccggtagg
     6121 aatggtcgat cgagacaagg tcgcctcgaa agcctggagc catggccgcg aggtttctgt
     6181 ttgcgaaagc ggcaccgacg atatcgacca cgtgacggtt cgttcgaccc cgtccacctc
     6241 gatgccagtc agcgtgccgc agccgcgaag gcgcacggtg gcgaactccg actcgagcag
     6301 catcttcggt gcggcggccc cgacgtcggg ctcggcgtcg agagcagcga ccagcggacg
     6361 cagccacccg ggcgaaacga ctgcgtcgtt gttgatcagc gccacgtagt cgacgccggc
     6421 gaggtcgcga agggcgcggt tgtttccttc ggcgaacccg tagttgtgtt cgttggcgac
     6481 gaacgtgacg cccggcaggt cggcgcggag gcgagcgagg gacccgtcga tcgatgcgtt
     6541 gtcgaccact acgatctcga ggcgctcggc cgggtagtcc gtggcttgca acgagcgaac
     6601 acatcgagcc gtcagccagg cagcgttcca gttgaggacg acgacgcgga ccgctgcggt
     6661 gaccgacggt ggggaggggg acattgacgc tggcgtcagc gcgccagccg ccgcgctgcc
     6721 ttgagggggc gcagcacggc ggtcccgacg cggttcgtca acgagtgctc gacatgggcg
     6781 agacggtcga tctcgacgcg catctggtgg atcagggctt cgagctcggc gttgcgggcg
     6841 cggagcgtcc cggcctgggc gatggcaccg aca
//
LOCUS       DAOSOR010000305         6849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_11148_length_6849_cov_3.937592, whole genome shotgun sequence.
ACCESSION   DAOSOR010000305 DAOSOR010000000
VERSION     DAOSOR010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6849
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11148_length_6849_cov_3.937592"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(390..533)
                     /locus_tag="PK710_15460"
     CDS             complement(390..533)
                     /locus_tag="PK710_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11177.1"
                     /translation="MSSLIESESLATKAVGSGQGGMGRKTVAWRGQGCAGSGGCHAVE
                     RVW"
     gene            1331..2377
                     /gene="pdxA"
                     /locus_tag="PK710_15465"
     CDS             1331..2377
                     /gene="pdxA"
                     /locus_tag="PK710_15465"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOT11178.1"
                     /translation="MQTIDSAMPILVTMGCPSGVGPEIALRAVLQARQKGLDCRIVGD
                     RGTLAAAASRLDLDVDRIVGHRTHDDNSNLPIPILEPVAPLHADERKPGRPSMAGGAA
                     QLTWIDFATDWVLARKAAALVTGPVSKEVIATSGAPGADSFKGHTEHIARRIGQGTPV
                     MVFVADSFAVSLVTTHLPLRDVSSCLTIESISHATRQTVVLLARLGCQPARVLICALN
                     PHAGEGGMLGDEEMNVIEPAVQDAKAWVKQQGFEAIVDGPCPAESAFRIAMGGRYDGV
                     VAMYHDQGTIPMKLRYFGHAVNVTAGLPIIRTSVDHGTAYDIAGQGKADAGSLWEAIH
                     LADRLSQGRSKSHD"
     gene            complement(2374..3657)
                     /gene="eno"
                     /locus_tag="PK710_15470"
     CDS             complement(2374..3657)
                     /gene="eno"
                     /locus_tag="PK710_15470"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOT11179.1"
                     /translation="MSEITAVIAREVLDSRGNPTVEVEVMTDGGFGRAAVPSGASTGE
                     HEALELRDGDKKRYLGKGVQTAVKNIETSLGPSILGLDSLDQTAVDKVMLDADGTDNK
                     NKLGANALLGVSMAVARAAADTVGLPLWRYLGGISARTLPTPMMNIINGGVHADSGLE
                     VQEFMIVPHGFSNFPEALRAGVEVFHSLKSHLKKDGLTVSVGDEGGFAPRLTSNEEAI
                     QRIMRAIEGAGYQPGKQISIALDAAASEFYNKENKNYTFDKKTVSADALVDIYSQLCE
                     KYPIVSIEDGCHEDDWDAWKKLTDKVGNKVQLVGDDLFVTNPKRLIMGIQKQVANAIL
                     IKLNQIGTVTETLDTIRLAAAHGYASVISHRSGETEDAFIADLAVATNAGQIKTGSLS
                     RSDRIAKYNQLLRIHEELDKGAVYLGHRVFGCNKF"
     gene            3941..4153
                     /locus_tag="PK710_15475"
     CDS             3941..4153
                     /locus_tag="PK710_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11180.1"
                     /translation="MAEDNAPKPVFCLRNHTSKDASEHLACPYCFGKKRETIEEAERK
                     DFCDFDPDQDPITFGFPDSSSRNAGT"
     gene            complement(4156..5508)
                     /locus_tag="PK710_15480"
     CDS             complement(4156..5508)
                     /locus_tag="PK710_15480"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT11181.1"
                     /translation="MDNKQFGSCRVTNALASGHVTEVFRGVQHPLGREVAIKTLKPSI
                     APTSPFALSLEREARILSHLRHDNIARILDHGRDGHAMWFSMEYVDGLTLRQLLSRVE
                     KLDVPCAVAIAVELARALSHAHANGVIHCDVRPSNVIVGRDGRVVLIDFGAAYAEAMP
                     TSPEPLDVNAALAAPSYMSPEQILGETVDPRSDVFAMGVVLYELLSGRRPFEATDQRT
                     LAHCVRHDEPKSLDRATTPRALSQIVARCLQKLPADRYASAKELCAALEEAFGNLSSS
                     PRRQVITAALVRARIIDRPVYVDEDPADRPGDLMVTPSVMPALRLQLMMLALLVVGGV
                     AIHFVFRSEIEAQATSGQGTLLLVPARAGSLLVVAQPWAHVLVDGLQVETTPFARPIP
                     LSAGLHHVTLRHPNAPDERRTVQINPGERVLLDVVMQVRKPPKPESSVPAPLPSASTP
                     "
     gene            5871..>6849
                     /locus_tag="PK710_15485"
     CDS             5871..>6849
                     /locus_tag="PK710_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11182.1"
                     /translation="MNDRESSLPPSPATSTAVPRKQGSIQSLWTWLAEYFVGEAPSFW
                     IAMVPMLILSALLFTRSLTSNYIFDEQEALLANPYVNGTKLNFADAVRRDFWGLPPDR
                     SVGSYRPLPNFVWRGVWDGQKALTHGVNWTLQRLEPIAIQARRPAHRPGWFSWPNLAG
                     LLIGGPALAALMIQITRAKSRRNRWATAAAAVLVGSLVASGSLGWLFTLSGGAGVAAL
                     LLLLLPVIALPTAVVGGLGLFALAILPTQPHPPVMQPWLFHWLNVLFHAANGAVLVCV
                     VHGITRRRGLAWLTGTIFVTSAVLTEAVSGVVGIADVMGGLGASLALLSL"
BASE COUNT         1524 a         2141 c         1780 g         1404 t
ORIGIN      
        1 ccgtcaagga aagtgcaggt ctttcgtcat gcaccatccc aaagccgcaa ggacaacgaa
       61 tgggcagaaa cgacgacctt cgataagtat agtccccaac gagtacccat gccttgcaaa
      121 ggtaacggtt ggcttctcac gatcgaatga acgcgctcat caaccatgaa acatcacaac
      181 cacatccgcc agcccaagcg catcacccac ggcaattggc tctttcacaa cccaatattc
      241 cctgtttttc tagttggcag agcgctttga accatggcgt atatgacatg atatgacatg
      301 gcgtgacaac caacgtgggg tcgttttttt cgccaacttc caccaacccg ttcaaccaac
      361 agcccctcgc tcaccccaac caccttccct caccataccc gctccaccgc atgacacccc
      421 cccgaacccg cacagccctg cccccgccac gctacagtct tccgtcccat gcccccctgt
      481 ccggaaccca ccgctttcgt tgccaacgac tcactctcga tcaaggagga catccgcaac
      541 cgcttgcttc gtaaagcgct ggaacagcga gaaaactaag ctgacctttt tctgaatatc
      601 gatcccgcgg ctcctactgc gtcgaagata attcgcccac gtttgcgaaa tcgccccaca
      661 acagcacccc tgcatatccc caaagcacag accaatctca cgccaaccgt tcgtgccatg
      721 gtcatcgcat cgagtgacgg tgccgtcatt cgcgatgtcc atccgatggt tcacttccct
      781 agcagcactg catgcgaaca ctacgacatg cgcatcacgg gctcccatgg gcgcggatat
      841 cgaacgtacc atttcatttt cgagcctcat ggcatcggaa cccacgcaaa aatcgcagcg
      901 tatcaagcgc tggggttatt ccaaacgcac tccgctcccg ccggcacatt ggatatcgtt
      961 ctcgcacccg agatagcgga atcctgtcgc atgaagccat tgggtatcgc ctcggagcgg
     1021 actacattcg aaaacgtgcc aagcacattg atggaagaca gatcgatatt tccagcggag
     1081 acttcgtttg ttctcttacg gaaagctttc tcatcgaaaa cggacgcatc accgcgccta
     1141 tttccggtgc gaacatcatc ggcaacggct ctgaaatctt tcgacgggtc aagcatgtcg
     1201 acttcgatgt cgccgcatcc ggtggcgtat ggatcaacag aagagcaggg caatccactc
     1261 cgaccagggt tggatgcccg accgttgcga tcgtcggcat tacggtgagt agcaaaaaaa
     1321 ggtgaaccca atgcaaacca ttgactccgc gatgcccatc ctcgtcacca tgggatgccc
     1381 ctcgggggtt gggccagaaa ttgctctgcg cgctgtcctt caagcccgcc agaagggttt
     1441 ggactgccgc atcgtgggtg atcgagggac tcttgccgca gccgcatcac gccttgacct
     1501 cgatgtcgat cgcatcgtgg gccaccgcac gcatgatgac aattcaaacc ttccgattcc
     1561 catcctcgag cccgtcgcac ccttgcatgc cgacgaacga aaaccaggac gcccatcgat
     1621 ggccggtggt gccgctcaac tcacctggat cgacttcgcc accgattggg tcctcgcacg
     1681 caaagccgcc gccttggtga caggacccgt atccaaagaa gtgatcgcca caagtggcgc
     1741 cccaggcgcc gattccttca aaggacatac cgaacatatc gctcgtcgaa tcggacaggg
     1801 aactcccgtc atggttttcg tggccgactc ctttgccgtg tctttggtca cgactcatct
     1861 gcccttgcgt gacgtatcct cctgcttgac gatcgaatcg atatcccatg ccacacgaca
     1921 aaccgtcgta ctgctggcac gactgggatg ccaacccgca cgcgtcctca tttgcgcgtt
     1981 gaaccctcat gctggcgaag gaggaatgct tggcgacgaa gagatgaacg tgatcgaacc
     2041 cgcggtgcaa gacgccaaag catgggtaaa acagcaagga ttcgaagcga tcgtcgacgg
     2101 cccctgccca gcggaaagtg cttttcgcat tgccatggga gggcgctacg acggcgtggt
     2161 ggccatgtac catgaccaag gtacgattcc catgaagctt cgatacttcg gccatgccgt
     2221 caatgtaaca gcagggctac cgatcattcg aaccagtgtg gaccacggca ccgcttacga
     2281 tattgcgggc caaggaaaag ctgatgcggg gtctttgtgg gaagccatcc atctagcgga
     2341 tcgtctgagc caaggacgat cgaaatcaca cgattagaac ttattgcagc caaagacgcg
     2401 gtgaccgaga tacacggcgc ccttgtccag ttcttcatga atgcgaagca attggttgta
     2461 cttcgcaatg cgatcggagc gcgacaacga tccggttttg atctgtccgg cattggtggc
     2521 cacggctaga tccgcgatga acgcgtcttc cgtttcaccg gaacgatggg aaatcaccga
     2581 ggcatacccg tgagctgcgg caagacgaat ggtgtccaag gtttcggtga cagttccgat
     2641 ttgattgagt ttgatgagga tggcgttggc cacttgcttt tggatgccca tgatcaatcg
     2701 cttgggattg gtgacgaata gatcatcgcc cacgagctgc accttgttgc caaccttgtc
     2761 cgtaagcttc ttccacgcgt cccaatcatc ttcgtggcaa ccatcttcga tggaaacgat
     2821 gggatatttt tcgcagagct ggctgtagat atcgacgagc gcgtcggcag aaaccgtctt
     2881 tttatcgaag gtatagtttt tattttcctt gttataaaac tcgctcgccg ccgcatccaa
     2941 ggcgatggag atctgtttgc ccggctggta tccggcgcct tcgatcgccc gcatgatgcg
     3001 ttggatcgct tcttcgttcg aagtcaaacg gggagcgaaa ccaccttcat cgcccacgga
     3061 aaccgtaagt ccatcctttt tcagatggct cttgagggag tgaaagactt cgacaccggc
     3121 gcgtaaggcc tcgggaaaat tggagaatcc gtgaggcacg atcatgaact cctgcacttc
     3181 gagtccggag tctgcgtgga ctccaccgtt gatgatgttc atcatcggcg tgggaagcgt
     3241 gcgagccgag attccaccca agtatcgcca taacggcaaa ccaaccgtat ccgccgccgc
     3301 acgagctacc gccatcgaca ctcccaacag cgcgttagcc ccaagcttgt tcttgttgtc
     3361 ggtgccatcg gcatccaaca tcaccttgtc aaccgccgtc tgatccagcg aatcaagccc
     3421 caatatcgag ggtccaagcg aggtttcgat atttttgacc gcggtctgaa ccccttttcc
     3481 caagtaccgc tttttgtccc catcccgaag ttcgagagcc tcatgctccc ccgtggaagc
     3541 accggaggga accgccgccc gtccgaagcc accatccgtc ataacctcta cctcaaccgt
     3601 cgggttaccc cgagaatcga gtacttcgcg tgcaatgacc gccgtaattt ccgacatggt
     3661 tcttctcctt gcgaaaccga cagcaccaag ggacagaaag cttcaccccg aaccgatatt
     3721 accagaggcc ctagcttcaa ccctctgccg aatgagttcg agcgcaccct aatgccacgg
     3781 gcccaaggac gccagccccg ttgcacgaga cgatgtcgat cgattggccc tcgctcgaga
     3841 tggttgaccg ccggttacgc cccaccgagc tacaccatca aagacgaccc ttcgcctggc
     3901 gcttgacctt cccatgtccc ggcaccaagc ttggatcgat atggctgaag acaacgcacc
     3961 caaacctgtt ttttgcctgc gcaaccatac gagtaaagat gccagcgagc atctggcttg
     4021 cccgtattgt ttcgggaaaa aacgagaaac catcgaagaa gccgagcgaa aagacttctg
     4081 cgacttcgac ccggaccagg acccgattac gttcggcttt ccggactcga gttcacgtaa
     4141 cgccggaaca tgacgttagg gcgtcgaggc ggaaggcagc ggtgccggta ctgaactctc
     4201 cggtttcggg ggttttcgca cctgcatgac cacatccaga agcactcgct cccccgggtt
     4261 gatttgcacc gttcgacgtt cgtccggcgc attcggatga cgaagcgtca catgatgcaa
     4321 cccggcagaa agcgggatgg gtctggcaaa aggcgtggtt tccacctgca aaccgtccac
     4381 cagcacatga gcccaaggct gcgccaccac gagcagggat ccggctcggg cgggaaccaa
     4441 caacagcgtg ccttgacccg aggtggcctg cgcctcgatc tccgaacgaa atacgaaatg
     4501 aatcgctact cctcccacca cgagcaaagc aagcatcatg agttggagcc gcagcgcagg
     4561 catcacactc ggtgtaacca tcaaatcccc aggccgatcc gcgggatcct cgtccacata
     4621 cacaggacga tcgatgatcc gcgcccgaac caacgccgcc gtgatgacct gacgccgagg
     4681 tgaggaagac aagttgccga aagcctcttc cagcgcggcg caaagttctt tggcagaagc
     4741 atagcgatcc gcaggcagct tctgcagaca tcgcgccacg atctgactca gcgccctggg
     4801 cgtcgtggct cgatccaaac tcttcggctc atcgtgacgc acacaatgcg ccagcgttcg
     4861 ctgatcggta gcttcaaacg gacgacgccc cgaaagcagc tcatagagca ccacccccat
     4921 ggcaaaaaca tcgcttcgcg gatcgaccgt ctcaccaagg atttgctccg gtgacatata
     4981 cgatggagcg gccaatgccg cgttgacgtc gagcggctcg ggtgatgtgg gcatcgcttc
     5041 ggcatacgca gccccaaagt cgatgagaac cacccgacca tcgcgcccca cgataacgtt
     5101 agacggacga acgtcacagt gaatcacccc attcgcatga gcatgggaaa gcgctcgggc
     5161 cagttcgaca gcaatcgcca ccgcacacgg cacatcgagt ttttccacac gcgacaacaa
     5221 ttgccgaagg gttagcccat cgacatactc catggaaaac cacatcgcat gaccgtcgcg
     5281 gccatggtcc agaatgcgcg cgatattgtc atgacgcaga tgggataaaa tccgtgcctc
     5341 ccgctccagc gacagagcaa agggagacgt gggcgctatc gaaggcttca gcgttttgat
     5401 cgctacttca cgcccaagag gatgctgcac accgcgaaac acctccgtga catgccccga
     5461 agcaagggca ttggtaacgc gacaggagcc gaattgcttg ttgtccatgg tagcaaagcc
     5521 tgggaaagcc ggcttcttgg gcgagccaat ctaccactac ccgggccaga ggatagagcc
     5581 gatccatggc ttatcatgcc tcgaccgccc cttgtccttc gccctttgcg gaacgagccg
     5641 ttcgcgctac gaacgggcag gtgaaacgtg ttttttttgc tgcctttgcg ttgggcttcg
     5701 aacggatttg tcaaaattca accgccagtg gtaaccagac gcatcctcgg ctcgtattcc
     5761 aacgatgacg cgctttgatg ggtcgccatg cgcaatgaaa tacatattca tgacggaaaa
     5821 gcccaacgtt gcactcacga tcgatctttt tgcctatcta cgggtcccgc gtgaacgata
     5881 gggaatcgag cctccccccc tctcccgcca cgagcacggc cgtgcctcga aaacagggga
     5941 gtatccaaag cctgtggacg tggctcgcgg aatatttcgt gggagaggcc ccctcgttct
     6001 ggatagcgat ggtgcccatg ctcatcctgt ccgcgctgct tttcacccga agcctgacct
     6061 cgaactatat tttcgatgaa caggaagccc ttctcgccaa cccctacgtc aatggcacca
     6121 aactcaattt tgccgacgcg gtccgaaggg acttctgggg actgcctccg gatcgaagcg
     6181 tcggttcgta tcgccccctg cccaacttcg tatggcgtgg ggtgtgggac ggacaaaaag
     6241 cgctgaccca cggcgtcaat tggacccttc aacgactgga gcccatcgcc atccaggctc
     6301 gccgtccagc ccatcgtccc ggatggttca gttggccgaa cctggctgga ttgctcatcg
     6361 ggggtccagc cctcgccgcg ctcatgatcc agatcacacg agcgaaaagc cgtcgaaatc
     6421 gctgggcaac ggctgcagcg gccgtgcttg tgggaagctt ggtagcttcc ggctcgctcg
     6481 gttggttgtt caccctttcg ggaggcgccg gagtcgccgc cctgctgctc ctgctcctgc
     6541 ccgtcatcgc ccttcccacg gctgtcgtcg gcggactcgg gttgtttgcg ctcgctatcc
     6601 tgcccacaca accgcatcca ccggtcatgc aaccgtggct atttcattgg ctcaacgtcc
     6661 tttttcatgc ggccaacggt gccgtgctgg tatgcgtggt ccacggcatt acacgacgtc
     6721 gcgggctcgc ttggctgacg ggaacgatct ttgtcaccag tgccgtgctc accgaagccg
     6781 tcagtggagt cgtgggtatc gccgatgtga tgggagggct gggagcgtcc cttgcgctgc
     6841 tgtctttgc
//
LOCUS       DAOSOR010000306         6795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_11261_length_6795_cov_4.401929, whole genome shotgun sequence.
ACCESSION   DAOSOR010000306 DAOSOR010000000
VERSION     DAOSOR010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11261_length_6795_cov_4.401929"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PK710_15490"
     CDS             <1..703
                     /locus_tag="PK710_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11183.1"
                     /translation="MPDDLRRALERQAQVHLLHETEEISGFALAWVERGELSVMSLVS
                     DMTAVVLGAGQALRNRGTVGEPVPLRLVCLSDSARVATWTEQVVDDSFEALPWVEEEL
                     REQADRTHVRIGVTLGPLGEAFDPPGLQEVLARLEPKHYLEGEVVVAAGDVIKGLMIL
                     GLGVLERVDAEGSVVSSLEMGTILFPTTLLSMEQVPWTVRAGTGGAVVMMAERSMVEE
                     MIMTFPPLLERLVRG"
     gene            complement(710..1294)
                     /locus_tag="PK710_15495"
     CDS             complement(710..1294)
                     /locus_tag="PK710_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11184.1"
                     /translation="MKRITRPGPYLTLAGLFCSSSGCGASYQALYEGEVRFEHCYRLD
                     EEPSIPLTQRRECWREWTQFYTFGQTRDRLEYALRRQRELTTQIENPHAQPDEISDIK
                     TLSSNPMATPSPTNPFAPPPHLQNENSPSTPPADPIPWLESLASNPQPTVQSCLMQCM
                     SRWKTCTDTCSHTNDECRKGCDSAFKACSQRCLE"
     gene            1386..2132
                     /locus_tag="PK710_15500"
     CDS             1386..2132
                     /locus_tag="PK710_15500"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOT11185.1"
                     /translation="MSRGGPNERLLGRAAGWSLLSLRVFANSIAVFTGLVEDVGQLLS
                     RTPRGPGARLRVATRLSPLVLGESIAVMGVCLTVQRILDGAFEADASGETLVRSTLGR
                     LPVGHGLHLERAVAVGGRFGGHIVAGHVDGLGRLVEKRPLGEAVDLAFSYDGHLAALL
                     AEKGSVAVDGVSLTINAVDAKVFHVAVIPHTQEKTLLEKMSVGMEVNLEADVLARYVA
                     RWLEVGRSAAGSGGTTDDSLLSRLASSGFL"
     gene            2163..3302
                     /gene="ribB"
                     /locus_tag="PK710_15505"
     CDS             2163..3302
                     /gene="ribB"
                     /locus_tag="PK710_15505"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HOT11186.1"
                     /translation="MTSRQLMIDPELLQRVHRALDEIRAGRMIILVDDEDRENEGDLT
                     MAAEAVTPEAINFMATHGRGLICVTLTEEQIERLRLPMMAGPNHDGPPLGTAFTVSIE
                     ARHGVTTGISAADRAHTIRVAVRPDARPQDIVSPGHIFPLRARKGGVLVRTGQTEGSV
                     DLARLAGLNPAGVICEIMNDDGTMARLPDLEIFAKKHGLTILSIADLIQYRTQTERLV
                     HRIAEGILSIDETKSDWKGFVYETAVDQQQLLALVKGDLQQAKEPVLCRVHRGSTIGD
                     IFSSTDSEGGHNLRTALRRIEEAGCGVVLYLPPRGDILREFKSYQKSEKVLESKDAPL
                     REFGLGAQVLADLGLSRIKILTDNPRKIAGIAGYGLEIVESAPIR"
     gene            3306..3815
                     /gene="ribE"
                     /locus_tag="PK710_15510"
     CDS             3306..3815
                     /gene="ribE"
                     /locus_tag="PK710_15510"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOT11187.1"
                     /translation="MQRSDMQDKPKLIEGALVVPPNARFAIVASRFNSFIVDRLIEGA
                     VDSLLRHGCELTNISVVRVPGAWEMPPIVRRIAESGNVDGVIAVGTVIRGSTPHFDYI
                     AAEVSKGLAHTGLDNPKVPIGFGVLTTDTIEQAVERAGTKAGNKGFDAALSVIEMVAL
                     RRVLDDAGC"
     gene            3879..4628
                     /locus_tag="PK710_15515"
     CDS             3879..4628
                     /locus_tag="PK710_15515"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOT11188.1"
                     /translation="MIPLRDRLPSRTTPWVNYGLIAANVLAFLWQLASADVGNTALAY
                     DWGFVPERFLKDPVAQVMTVWTAMFLHGSWLHIAGNMLFLWVFGDNVEDALGHGRYLL
                     FYLAGGFLAAMAQCLAEPSAAVPMVGASGAIAAVLAGYVTLYPKARILVLFPVFVVFL
                     FFEFPAWLVMLEWIVLNVVQALVSVGDRAGAGVAWFAHVGGFLAGLFLIRLGMVGRER
                     IAYEKWRGFYTREQPSRSLRVRYRRPPGSFR"
     gene            4625..5218
                     /locus_tag="PK710_15520"
     CDS             4625..5218
                     /locus_tag="PK710_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11189.1"
                     /translation="MTIASSILLRNVVRCEPLMNWQSEFRWWIRVKILALVLCSVGAS
                     WLGCSSTHFDGRHYRRGPLEFTLGPAPKSWRNIEVSDALLAFRDDERHATIAVHGRCG
                     KDGDDVPLEALTQHLFIHFTERETLDQRLLSMDDREAMRSEITAKLDGVPKQFVVYVL
                     KKDGCVYDFFYIGESSMAATGVEAFDNYVATFRTYER"
     gene            5222..6100
                     /locus_tag="PK710_15525"
     CDS             5222..6100
                     /locus_tag="PK710_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT11190.1"
                     /translation="MTDEQRPRHPNTPSTEDRQRSSLALLAHKGPPSSRFTNLLVDQL
                     LGFVDHLGACSLMILRTARALVTPPFDFRAVVYQLESVGVKSVSIASVTSVFIGMVMA
                     VQFAISLQKFGAMEYTGRVVGLSFSRELAPTLTAVIVGGRVGAGMAAEVGSMAVTEQI
                     DAIRALGADPVKKLVLPRVVALVLGMPVLALLALVFGFTGAMMVVGAQFNIPPGFFLS
                     SAIDSVTMNDFMSGICKCPFFGAIIAFVGCYFGLTTTGGTEGVGKSTTRTVVVVSIAI
                     LIADFFLTKLYFLAFG"
     gene            6231..6791
                     /locus_tag="PK710_15530"
     CDS             6231..6791
                     /locus_tag="PK710_15530"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOT11191.1"
                     /translation="MCNESSSDGSTVRRRGRKKKRKDGLLTTGDMARLSNSTLRTVRF
                     YEEADILHPVVRSDGGHRLFSSVELEKLLLVTDMRAAGLSLDEIKTILAIKTTSKTGV
                     EAAQRVGKYLREQIRQMEEKVVVLQRLRDDFGRADELFHRCHLCQTQGTFPSSCGICG
                     VMDHPELLPRTVRVLWNLGGAGSDKE"
BASE COUNT         1285 a         1566 c         2276 g         1668 t
ORIGIN      
        1 catgcccgac gatcttcgac gtgcgttaga gcgtcaggcg caggtgcatc ttcttcatga
       61 aaccgaagag atttcggggt ttgcgctggc gtgggtggaa cggggcgaac tatcggtcat
      121 gtctttggtc agtgatatga ccgcggtcgt actcggagcg ggacaagcgc tgcgcaatcg
      181 tgggaccgtg ggtgagcccg ttccgttgcg tctggtatgc ctttcggatt cggctcgtgt
      241 ggctacctgg accgagcagg tggtggacga tagtttcgaa gcgcttccgt gggtggagga
      301 agagcttcgg gagcaggcgg atcggacgca cgtccggatc ggggtaacgt tggggccgtt
      361 gggggaagct ttcgatccgc caggattgca agaagtcttg gcacgattgg aaccgaagca
      421 ttatctcgag ggtgaggtgg tcgtagcggc gggtgatgtg atcaaaggat tgatgattct
      481 tgggctgggt gtgcttgaaa gggtcgatgc ggaagggagc gtggtttcgt cgttggagat
      541 ggggaccatt ctttttccga cgacactgct atcgatggag caggtaccgt ggacggtgcg
      601 ggcaggtact gggggggcgg tggtcatgat ggcggagcgg tccatggtgg aagaaatgat
      661 catgaccttt ccaccgttgt tggagcgact tgtgagagga tgactgcttt tactcgaggc
      721 agcgttggct gcaggctttg aaagcactat cgcaaccttt tcggcattcg tcgttggtgt
      781 gcgagcatgt gtcggtgcat gttttccagc gagacatgca ttgcatcagg caagattgga
      841 cggtgggttg cgggttggag gccagggatt ccaaccatgg gatgggatct gctgggggtg
      901 tagacggtga attttcgttc tgaaggtgtg gtggaggcgc aaagggattc gtgggagagg
      961 gggtagccat tgggttgctg gacaatgttt tgatatcgga tatttcgtct ggttgtgcgt
     1021 gcggattttc gatttgggtg gtgagttctc gctgacggcg aagagcgtat tccaaccgat
     1081 ctcgcgtttg gccgaaggtg tagaactggg tccattcccg ccaacattca cgtcgttggg
     1141 ttagcggaat cgaaggctct tcatcgagcc ggtaacaatg ctcgaatcgc acttcccctt
     1201 cgtagagggc ttgataactg gcgccgcatc cgctggatga gcagaaaagt cccgcaaggg
     1261 tgaggtatgg cccagggcgg gttatccgtt tcaacctgac gacgatgccc atgcagacca
     1321 aagggtgcga tccttggcga ctgaaggcaa gggggagttg aaaccgaagg tttttgtacg
     1381 tcgtgatgag cagggggggc cctaacgaac ggctattggg gcgagcggcg ggatggtcgt
     1441 tgctatcatt gcgggttttc gctaactcca tcgccgtgtt tactgggctc gttgaggatg
     1501 ttggtcagtt gctctctcgc acaccgcgag gcccgggggc ccggttgcgc gtagcgacac
     1561 gtctttcacc gctcgtactg ggggagtcga ttgcggtcat gggagtctgt ctcacagtgc
     1621 aacgaatcct cgatggggcg tttgaagcgg atgcctcggg cgagacgttg gttcgcagta
     1681 cgcttggtcg attgcccgtg ggtcacggat tacatctcga gcgtgcggtt gcggtggggg
     1741 gaaggtttgg agggcatatc gttgcggggc atgtcgacgg tttgggacgt ttggtggaaa
     1801 aaaggccctt gggtgaggct gtcgatttgg cattttccta tgatggccac ctcgctgcgt
     1861 tgctggccga aaagggatct gtcgcggtgg acggtgtgag tctcacgatc aatgcggtcg
     1921 acgcgaaggt atttcatgtg gcggtgattc cccatacgca agaaaagacg ttgttggaga
     1981 agatgtcggt gggcatggaa gtgaatctcg aggccgacgt gcttgcacgg tatgtggcgc
     2041 gttggctcga ggtgggccgg tcggccgcag ggtcgggtgg gacaacggac gacagtctgc
     2101 tgtcacggtt ggcctcatcg ggatttttat gaagtgggtg agaacacgga gtcgaaatag
     2161 tcatgacctc acgccagttg atgatcgatc cggagttgct tcagcgggtg catcgcgcgt
     2221 tggacgaaat tcgtgcaggg cgaatgatca ttttggtgga cgacgaagat cgggaaaacg
     2281 aaggtgattt gacgatggcg gcggaggcgg tcacgcccga agcgatcaac ttcatggcga
     2341 cccatggcag agggttgatt tgcgtcacgt tgaccgaaga gcaaatcgaa cgattgcgac
     2401 tgccgatgat ggcggggccc aaccatgacg gtccgccctt gggtacggcc ttcacggtat
     2461 ctatcgaggc ccgacacggt gtgacgacag gcatcagcgc ggccgaccgc gcgcatacga
     2521 ttcgggtggc ggttcgtccc gatgctcgcc cacaggatat cgtgtctccc ggacatatct
     2581 ttcctttgcg agcacgaaaa ggcggggtgc ttgtgaggac cggtcagacg gaaggatcgg
     2641 tggacttggc ccggttggcc ggactgaatc ccgccggtgt catctgtgag atcatgaacg
     2701 atgacggaac gatggcgcgt cttccggatc tcgaaatttt tgcgaaaaaa cacggactta
     2761 ccattttgag catcgcggat ttgatccagt atcgcacgca gacggagcgg ttggttcatc
     2821 gcattgccga agggattttg tcgattgacg aaaccaagtc ggattggaag gggttcgtgt
     2881 acgagacggc ggtggatcag cagcagcttc tggccttggt caaaggggat ttgcagcaag
     2941 ccaaggagcc ggttttatgc cgcgtgcatc gaggttccac gattggggat atatttagtt
     3001 cgacagattc ggaagggggg cacaacctgc gtaccgcgtt gcgacggatc gaggaagcgg
     3061 gttgtggcgt ggtgttatat ctgccgcctc ggggggatat tcttcgtgaa ttcaagagct
     3121 atcaaaagag cgaaaaggtg ttggaatcca aagatgcgcc cctgcgggag ttcggtcttg
     3181 gtgcacaagt cctggcggat ctcggattga gtcgaatcaa aattttgacc gataacccaa
     3241 gaaaaattgc cgggatcgct ggctatggtc tcgagatcgt ggaaagcgct cccattcgtt
     3301 gagccatgca gaggagtgat atgcaagaca aaccgaagtt gatcgaaggc gcgctcgtcg
     3361 ttccccccaa cgcacgcttc gccatcgtgg ccagccggtt caacagcttc atcgtcgatc
     3421 gattgatcga aggagccgtc gattcgctgc tccgacatgg ctgtgaactc accaatattt
     3481 ccgtcgttcg cgtgccagga gcctgggaaa tgcccccgat cgtgcgtcga attgcagaat
     3541 cgggcaacgt cgacggtgtc attgccgtag gcacggtgat ccgaggctcg actccgcact
     3601 tcgactatat tgccgcggag gtctccaaag gtcttgcgca tactggtttg gataatccca
     3661 aggttcccat tgggtttggc gtgctcacca ccgataccat cgagcaggcg gtggaacgag
     3721 cgggaaccaa agccggcaac aagggattcg acgcggcctt gagtgtgatt gaaatggtgg
     3781 cactgcgccg ggtcttggat gatgccgggt gttgagacct tgcctgtcgt attcggatcg
     3841 ccgattcttt ggactgcgct tacctttcct gacgcctcat gattccgcta cgagacaggc
     3901 ttccgagccg cacgacgccc tgggtcaact atgggttgat tgccgccaac gtactagctt
     3961 ttctttggca attggcgtcg gcggatgtgg gtaatacggc tcttgcctac gattggggat
     4021 ttgtcccgga acgatttctc aaggatccgg tagcgcaggt gatgacggtg tggaccgcca
     4081 tgtttttgca tggtagctgg ctccatatcg ccggaaatat gctgttttta tgggtatttg
     4141 gagataatgt ggaagatgcc ctcggccatg gtcgctacct attgttttac ttggccgggg
     4201 gatttctcgc ggccatggct caatgcctgg cggaaccaag cgccgcggtg ccgatggtgg
     4261 gtgcttccgg ggccattgcc gccgtgcttg cgggctacgt aacgctctac cccaaagcca
     4321 gaattctcgt tctttttccg gtatttgtgg tctttctctt cttcgaattc cccgcgtggc
     4381 tcgtcatgct cgaatggatt gtcctcaatg tcgtgcaagc tttggtgtcg gtgggagata
     4441 gggcgggtgc cggggttgcc tggtttgccc atgtcggtgg atttttggcg ggtctctttc
     4501 tcattcgtct cggtatggtg ggtcgtgaac gcattgctta cgaaaaatgg cgaggcttct
     4561 acactcgaga gcaaccgtcc cgatcccttc gcgttcgcta tcgacggcct cccggatcgt
     4621 ttcgatgacc atcgcctcgt cgattctgct tcgaaacgtg gtacgttgtg agcctttgat
     4681 gaactggcaa tccgagtttc gatggtggat acgggtcaag atactggctt tggtgctgtg
     4741 tagtgtcggg gcatcgtggc ttggatgctc gtccacgcac ttcgatggtc ggcattatcg
     4801 acggggtccc ttggagttca ctcttgggcc tgctccgaaa tcatggcgga acatcgaggt
     4861 gtcggatgcg ctactggcgt ttcgggatga tgaacggcat gcgaccattg cggtccatgg
     4921 tcgttgtggg aaagatggcg atgacgttcc cctcgaggcg ttgacacagc atctattcat
     4981 tcactttacc gagcgggaga cgttggatca gaggctgctt tccatggatg atcgggaagc
     5041 gatgcgctcg gagatcacgg ccaaactcga tggtgttccc aaacaattcg tggtgtatgt
     5101 gctgaagaaa gatgggtgtg tttacgactt tttttatata ggcgagtctt cgatggccgc
     5161 gacaggggtc gaagccttcg acaattatgt ggccacgttt cgaacgtacg agcgttgagc
     5221 gatgacagac gagcagcgac cgcggcatcc gaatacgccg tcgacggagg atcggcagcg
     5281 ctcgtcgttg gcgttgcttg cccacaaagg gcccccatcg tcacgcttta cgaaccttct
     5341 ggtggatcag ttgttggggt tcgtggatca tcttggggcg tgcagcctga tgattttgcg
     5401 cacggcgcgc gcgttggtga cccctccctt cgattttcga gcggtggtgt atcagctcga
     5461 gtcggtggga gtgaagtcgg tgagcattgc atcggtcaca tcggttttca ttggcatggt
     5521 gatggccgtt cagttcgcga tcagtcttca aaagttcggg gcgatggaat acaccggacg
     5581 ggtggtgggg ctgtcgtttt ctcgtgagct agcgccgaca ttgacagccg tgatcgtggg
     5641 agggcgtgtg ggggcgggga tggccgcgga ggtggggtcg atggccgtga ccgagcagat
     5701 cgatgccatc cgggcgttgg gagccgatcc ggtcaaaaaa ctagtgttgc cgcgcgtggt
     5761 ggcactggtt ctgggcatgc ccgtgctggc cttgctagcg ctggtatttg ggttcacggg
     5821 tgcgatgatg gtcgttgggg cgcaattcaa catcccgccg ggctttttcc tgagttccgc
     5881 catcgactcg gtcaccatga acgattttat gtcggggatc tgtaagtgcc cgttttttgg
     5941 tgcaattatc gcatttgtgg gatgctattt cggcttgacg accacggggg gcacggaggg
     6001 ggtgggtaag tcgacgacgc gaacggtggt ggtggtttcc atcgcgatcc tcatcgcgga
     6061 cttctttctc accaaactgt actttctggc ttttgggtaa agtaccctga ccctggtttt
     6121 ttcgtcgaag ataggggtgc tgcgttgcgt ttgctctggt tttgcaggga gcagcccttg
     6181 acatcgcgaa acggagcttt caggatgggg taacgtaaag gaaggttgtc gtgtgcaacg
     6241 aaagctcttc ggatggctcg acggttcgtc gtcgagggcg gaaaaagaag aggaaagatg
     6301 ggttgttgac aacgggggat atggcgcgtc tgtcgaattc gacacttcgg accgtccggt
     6361 tttacgagga agcggatatt cttcatcctg tggttcgcag cgacggcggg caccgtttgt
     6421 tttcctccgt ggaattggaa aagctgttgc tcgtgacgga tatgcgggcc gcgggattgt
     6481 ctctcgacga gatcaaaacc attttggcga tcaaaacgac gagcaagaca ggggtggagg
     6541 cggcgcagcg agtgggaaag tatcttcgtg agcagatacg gcagatggaa gaaaaagtgg
     6601 tggtgctgca acgtcttcga gatgattttg gtcgggcgga cgaactcttc caccgatgcc
     6661 atttatgtca aacgcaaggc acgtttccgt cttcctgcgg gatttgtggt gtgatggatc
     6721 atcctgagct gttgccgcgt acggttcggg tattgtggaa tcttggtgga gcgggttccg
     6781 acaaggaatg atcgt
//
LOCUS       DAOSOR010000307         6792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_11269_length_6792_cov_4.402256, whole genome shotgun sequence.
ACCESSION   DAOSOR010000307 DAOSOR010000000
VERSION     DAOSOR010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11269_length_6792_cov_4.402256"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /gene="rpsF"
                     /locus_tag="PK710_15535"
     CDS             complement(<1..532)
                     /gene="rpsF"
                     /locus_tag="PK710_15535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOT11192.1"
                     /translation="MAEVNMAQTGAKEYEAIYILRPDIDPDTATRVSNRVTEVLARDN
                     AKLIKVESWGRRKLAYPVRKYRRGIYVFLRFLGKTSLVAEFERNLRLQKDAVLKFMTI
                     KVGDAAIDESVEISPEDIQFAAVEPMTEEEMDESREKLLGLVESHDRYSRDDRGMGAR
                     MDEDLDDEMDEGLDDDE"
     gene            complement(617..1300)
                     /gene="pth"
                     /locus_tag="PK710_15540"
     CDS             complement(617..1300)
                     /gene="pth"
                     /locus_tag="PK710_15540"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOT11193.1"
                     /translation="MLVIAGLGNPEPRYARNRHNIGFEVVQAFAEVVGADPWRSKFQG
                     EIARCHVGQTDVLLVRPMTFMNRSGECLQAVTSFFRIPPSDLLVVHDELDLPFGTLRL
                     KPGGGHAGHNGLRSIIQHLGSDFARLRCGIGKPPAGFRGEVADYVLSDFDANERDALA
                     DVVDRAVKALRMVLKRGLGEASKALNTRPKPPKPSPDPIPVEHDAEPGQADQREIPQE
                     GLAPNSKRS"
     gene            complement(1355..2014)
                     /locus_tag="PK710_15545"
     CDS             complement(1355..2014)
                     /locus_tag="PK710_15545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOT11194.1"
                     /translation="MEFHKIDASLRAQRGKGPSARLRREGVIPAIAYGSGHEATAITV
                     NPKELLAAVTGPWGRNTVLQVAVDGAEPFPALLADHAYHPVSRDLVHADFLRIDLSKP
                     VEVDVPLTTTGKAAGVIEGGTLRQIFRKLPISCLPSAIPQFIEFDVTEMKQNDVRKVS
                     DLTVPEGVTIRLPIEQTVVAVDAAVQEEVEAAAEGEGAAPAAAPAEAEKKAPEKKADK
                     K"
     gene            complement(2080..3123)
                     /locus_tag="PK710_15550"
     CDS             complement(2080..3123)
                     /locus_tag="PK710_15550"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOT11195.1"
                     /translation="MIRASNWAPATGPSGAAWRVGFTARPIHEGRRDVFRKVCIFSGN
                     ANPALTQEIVKFLEIPLGQIRVSRFSDGETFCEVQENVRGVDSYVIQPTSSPVNENFM
                     ELLIIADALRRASAGSITAVVPYYGYARQDRKVAPRTPITSKLCADLMVSAGVNRVLC
                     VDLHAGQIQGFFNIPFDHLFSMPVMLENYIKRHYDSSAVVVSPDAGGVERARAYAKRF
                     GASLAIIDKRRERANISEVMHIIGEVSGRDCIIVDDMIDTAGTLCNSARALQQAGARK
                     VVACATHAVLSGPAVARIMDSPLSEVVVTNTIPLSEEAAQCGKIRVVSIARLLGEAIR
                     RIHHSDSISSLFA"
     gene            complement(3256..4074)
                     /locus_tag="PK710_15555"
     CDS             complement(3256..4074)
                     /locus_tag="PK710_15555"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HOT11196.1"
                     /translation="MNDPAVDAQMEKESARAEGGESDVSVRVTSGGERYFVTDGSATV
                     GPVDGELLARGIHAGRVPLDAMVWSAGWAQWRLVSDYASELGLMPSREETRASSMLAR
                     VSDLVPMQLPSTDVQVQALRDCRTLNEAASKWLEQAVAVTEADCGWVHVFSRQAGGAM
                     ITLEGKGPRAAFGVGRTIDPADHALRAAREGRLVMSEPMAGVVGAAISARLVATGVTP
                     ISVLMVPVVVENQLLVMLELGKDDGWFSATVAAVVEQLSKDLGMFARKRRWHQG"
     gene            4359..5156
                     /locus_tag="PK710_15560"
     CDS             4359..5156
                     /locus_tag="PK710_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HOT11197.1"
                     /translation="MAITALVLGILGWMCFGPIAGIAAMIVGALALWDIRASRGKKSG
                     AILAWVGLSSGAATCLLFAAVIGFFFHTLHRTAPALPPTSFTPSPPSTSLPAPPPSST
                     ATETREVRTVETHVGKITVVDIGEQESSLPSALRQQRLIAEREKQLLVLQTTSTECRP
                     CQGVAAALSAPAMQTALASVRLVRTDVHIFSQELDELGVPSELIPGFFLLGTDLTARD
                     GIHGGEWDDDVVDNIAPVLKEFVRGTLTRRRHPNIVSPTPKPKGTVL"
     gene            complement(5160..5999)
                     /locus_tag="PK710_15565"
     CDS             complement(5160..5999)
                     /locus_tag="PK710_15565"
                     /inference="COORDINATES: protein motif:HMM:NF015376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HOT11198.1"
                     /translation="MRSISPVRTIALAAAVLLVPVDVASAPPAPAAPTALFRTPHALS
                     VGAPNAGSLVRGSKLETSDHIRVVPDWNHPDYRWGLPELVQMLQRAAHHIHEKHGPCR
                     MSVGDLSCREGGSLRKHRSHQSGRDVDIAFYMVDRKGKPLYHSSFVAFGADGRAINHP
                     EARFDDTRNWALVATLLSDPKARVQQIFVSNDVRKRLLIEAERQQAPRRLRLEAARVL
                     IQPSGSSAHADHFHVRIACPPNQRGACESWPRSPTREIVTPDQQKPNKSARGKRRRAG
                     TEG"
     gene            6491..>6792
                     /locus_tag="PK710_15570"
     CDS             6491..>6792
                     /locus_tag="PK710_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11199.1"
                     /translation="MANNDDRALIQPLPEEDMDYILARCSQEFETLRGASIFITGASG
                     FVGSWLTQSLLWAQDRRNLGLRLVLLTRSSTAFAARQPGVAHHPSVHLLEGDMQSFP"
BASE COUNT         1471 a         2152 c         1754 g         1415 t
ORIGIN      
        1 cctcatcgtc gtcaagacct tcgtccatct cgtcgtccag gtcttcatcc atacgagccc
       61 ccattcctcg atcatcgcga gaataccggt cgtgagattc cacaagaccg agcaacttct
      121 cacgggactc gtccatttcc tcttcggtca tgggttcaac ggccgcgaat tgaatatcct
      181 ctggactgat ctcgacagac tcatcgatcg ctgcgtcgcc gaccttgatg gtcatgaact
      241 tgagcacggc gtctttttgc aaacgaaggt tgcgctcgaa ctccgccacg agcgatgttt
      301 tccccaaaaa tcgaagaaaa acatagatgc cgcgacggta tttgcgcacc gggtaggcga
      361 gctttcgacg accccaggac tcgaccttga tgagtttggc gttgtctcgt gcaagcacct
      421 cggtgacgcg attggacaca cgggttgccg tgtccggatc gatatccggt cgtagtatgt
      481 agattgcttc gtattctttg gcgcctgttt gcgccatgtt gacttcggcc atgatgactc
      541 ctttcggcct gtggcctccc acggatatgg gaagcaagga gatgggtgca atcacccagg
      601 tgtcggggcg gtgcttctag ctccttttcg aattcggcgc cagcccttcc tgtgggattt
      661 ctcgttgatc ggcttgtcct ggctcggcat catgctccac cgggattggg tcgggggatg
      721 gttttggagg ttttggacgc gtattgagag ctttggaggc ttctcccagc cctcgcttca
      781 ataccatgcg cagcgcttta accgcacggt ccaccacgtc ggccagggca tccctttcgt
      841 ttgcatcgaa gtccgacaag acgtaatccg ccacttcgcc acgaaaaccg gcgggaggtt
      901 tgccgatccc acagcgcaaa cgcgcgaaat ccgagcctag atgctggatg atcgaacgca
      961 aaccattatg tcccgcatgc cctccgccgg gtttcaaacg taaggtgccg aacggcaagt
     1021 cgagttcatc atgaaccacg agcaagtccg atggcgggat tcgaaaaaaa ctcgttaccg
     1081 cttgcaaaca ctcgcccgaa cgattcatga acgtcatggg tcgaacgagc agcacatcgg
     1141 tttgaccgac atgacagcga gcgatctcgc cttggaactt gcttcgccaa ggatcggcac
     1201 ccaccacctc ggcaaaggcc tgcacgacct cgaaaccgat attatggcga ttgcgggcat
     1261 accgaggttc gggattgcca agtcctgcaa tgacaagcat gagggatgcc cgtacgaaga
     1321 aaaaggcatt gcttcatcgc atgaaacgat gaagctactt cttgtcggct ttcttttccg
     1381 gagctttctt ctctgcctcg gcaggagccg ccgcaggagc tgccccttcg ccttccgctg
     1441 ccgcttccac ttcttcttgt acggccgcat ccacagccac aaccgtctgt tcgatgggaa
     1501 gacgaatcgt taccccttcg ggcaccgtaa gatcactcac cttgcgcaca tcgttttgct
     1561 tcatctccgt cacatcgaat tcgatgaatt gcggaatcgc agaaggcaaa catgagatgg
     1621 gcagcttgcg gaaaatctga cgaagggttc ccccctcaat cactccagcc gccttgcccg
     1681 tcgtcgtcaa cggcacatcg acttccaccg gcttcgacaa gtcgatccga aggaagtccg
     1741 catgcaccaa atcacgactg acgggatgat aggcgtgatc cgccaacaag gccgggaacg
     1801 gctctgcacc gtcgacagcc acctgaagaa ccgtattgcg accccagggt cccgtcaccg
     1861 ccgccaaaag ctctttggga ttcacggtga tagccgtggc ctcatgcccc gaaccataag
     1921 caatggccgg gatcacacct tcgcgacgaa ggcgggccga aggccccttt ccccgttgtg
     1981 cgcgaagcga agcgtcgatc ttatgaaact ccatcttgcc tgtctcttcc tagctaatgt
     2041 cttgcgcatc taccatgcgc tagccgtctt gagccgctct catgcgaaga gcgagctaat
     2101 cgaatcacta tggtggatgc gtcgaatcgc ctcgcccaac aatcgagcga tggaaacgac
     2161 gcggattttg ccgcattgcg ctgcctcctc ggacagcggt atggtgttgg taaccacgac
     2221 ctccgacaga ggtgaatcca tgatccgcgc tacggcaggt cccgaaagaa ccgcatgcgt
     2281 cgcacaagcc accacttttc gggcccctgc ttgctgaagc gctctggcgg aattgcaaag
     2341 cgtccccgcc gtgtcgatca tgtcatccac gatgatgcaa tcacgccccg acacctcacc
     2401 gatgatatgc atgacttcgg agatatttgc gcgttctcga cgtttgtcga tgatggcaag
     2461 cgatgcgccg aagcgtttgg cgtaggcacg cgcacgctcg actccgcctg catccggtga
     2521 aaccaccacc gcgctgctgt cataatgccg tttgatgtag ttctcgagca tgaccggcat
     2581 cgagaaaaga tgatcgaacg ggatgttgaa aaagccttga atctgccccg cgtgcaagtc
     2641 gacacataaa acacgattca cgcccgcgga taccatcagg tccgcacaaa gcttcgaggt
     2701 aatcggtgta cgcggcgcga ccttgcggtc ctgcctcgca taaccatagt acggaacgac
     2761 tgcggtgatg gatcccgcgg aagcacgacg caacgcatcg gcgatgatga gcaactccat
     2821 gaagttctcg ttcacgggag aactcgtggg ttggatcaca tacgaatcga caccgcgaac
     2881 gttttcctgg acttcgcaga acgtttcccc atcagaaaag cgcgaaacac gaatctggcc
     2941 gagaggaatc tcgagaaact tgacgatttc ctgcgttaaa gccggattcg cgttgccaga
     3001 aaagatgcat accttcctga acacgtcgcg cctgccttcg tggatgggtc ttgccgtgaa
     3061 accgacgcgc caagccgcgc cggaaggacc cgtggcgggg gcccaattcg aggcgcgaat
     3121 cactagcagc gacaaaacga gttgtcaacg ctgagtcatg ccccaacccc cactcgccac
     3181 ccaacacgaa ccgccacacg aaaagaaagc ctgccgagcc atatattaaa tcaacaaatt
     3241 caaatggtta caatatcatc cctgatgcca acggcgcttt cgcgcaaaca tgcccaagtc
     3301 cttggatagt tgctccacca ccgccgccac cgtcgcagaa aaccaaccat cgtccttgcc
     3361 gagttccaac atgaccaaaa gctggttttc caccaccact ggcaccatca ataccgagat
     3421 gggggtaacg ccagttgcta ctagacgcgc cgaaatcgct gctcccacca cccccgccat
     3481 gggttcactc atcaccagtc gcccctctcg ggccgctcgc aaggcatgat ccgccgggtc
     3541 gatcgtgcgc cccacgccaa aggccgcccg tggcccctta ccctcgagcg tgatcatcgc
     3601 tcctcccgct tgccgcgaaa aaacatggac ccaaccgcaa tccgcctccg tcaccgccac
     3661 ggcctgctcc aaccacttgc tcgccgcctc gttcaacgtc ctgcaatctc gaagcgcttg
     3721 cacctgcaca tccgtcgagg gcaattgcat cggcaccaaa tcactcacgc gcgcaagcat
     3781 cgaactcgcc cgcgtctcct cccggctcgg catcaacccc aattccgacg catagtcgga
     3841 caccaaacgc cattgcgccc accccgccga ccacaccatc gcgtccaaag gaacccgacc
     3901 cgcatgaatg ccgcgagcca acaattcccc atcgaccggc cccactgtcg cactcccatc
     3961 ggtgacgaaa tatcgttccc cacccgatgt cacgcgcacg gaaacatcac tttcaccacc
     4021 ctcggctctc gcagactctt tttccatttg tgcatcgacc gccggatcgt tcatgatacg
     4081 cccccaatca gacccaaacc tattggtacg tggtatgcgt acgtattcaa tttgatcacc
     4141 ttgtcaagac acaattgttc tgagttgtgg ttttctcacg gaaaggccaa cgacccgaag
     4201 gccgccgtca aaaacaccgg aatatcgtga ccccctccga gccccctcgc cccgagcctg
     4261 aacctacgcc cttccacgcc ccgcctccgt ggcgtgctga tgtgccaggc ataagcccag
     4321 gaatggccgc accagccccg cctgcaccga cgagcgccat ggcgatcacc gcattggtac
     4381 tcggtattct cggctggatg tgtttcggcc ccatcgcagg tatcgccgcc atgatagttg
     4441 gtgcgctagc cctgtgggat atccgagctt ctcgagggaa aaaatccggt gccatcctgg
     4501 cctgggttgg cttgtccagc ggcgcggcca cttgcctcct attcgcggca gttatcgggt
     4561 tcttctttca taccctccac agaactgctc cagctctccc ccccaccagc ttcacgccct
     4621 ctcccccatc gacatccctt cctgccccac ccccgtcgtc cacggctacc gagacccgcg
     4681 aagtccgcac cgtcgaaacc cacgttggaa aaatcaccgt cgtggatatt ggtgaacaag
     4741 agtcctccct tccatcggcc ctccgccagc aacggctcat cgccgaacga gagaaacaac
     4801 tgctcgtgct acaaacgaca tccaccgaat gccgtccttg tcagggggtg gcggccgcgt
     4861 tatccgcacc agccatgcaa accgccctgg cttccgtgcg gctggttcgc acggacgtcc
     4921 acattttttc gcaagaactg gatgaattgg gggtgccctc cgaactcatt cccgggtttt
     4981 tcttgctagg cacggacctg accgcacgcg acggaatcca tggcggtgaa tgggatgatg
     5041 atgtggtgga caacattgcc ccggtgctca aggaattcgt tcgagggacc ctgacccgac
     5101 ggcgtcaccc caatatcgta tcgcccaccc ccaagcccaa gggtacggta ttataaggat
     5161 tatccttcgg tacccgcgcg acgacgtttc cctctcgcgc ttttattcgg tttttgctga
     5221 tccggtgtga caatttcacg agttggggat cgaggccaag actcacaggc cccccgttgg
     5281 ttcggcgggc atgcgatgcg tacatgaaag tggtcggcgt gggccgagga gccggagggt
     5341 tgaatcagca cacgagcagc ttccaatcga agtcgtcgtg gtgcctgttg ccgctcggct
     5401 tcgataagca gccgctttcg cacgtcgttg gacacgaaaa tctgctgaac tcgcgctttc
     5461 gggtccgaaa gcaacgtggc aacgagggcc caattgcggg tatcgtcgaa acgcgcttcc
     5521 ggatggttga tggctcgtcc atcagcccca aaggcgacaa acgacgagtg atacaagggt
     5581 tttccctttc gatcgaccat gtaaaacgcg atatcgacat cgcgtccgct ttgatgcgag
     5641 cgatgtttgc gcagcgatcc cccttcgcga caggataaat cccccacact catgcggcag
     5701 gggccgtgtt tttcatgaat atggtgcgcg gcccgttgca acatctgcac gagttcaggc
     5761 aatccccagc gataatccgg atggttccaa tccggcacga cgcggatatg atcggatgtt
     5821 tcgagttttg atccccgcac gagactcccg gcattgggag cacccaccga caacgcatga
     5881 ggggttcgaa ataacgccgt cggagcggcg ggtgccggag gagcggaagc aacgtccacg
     5941 ggaacaagca ggaccgcggc cgccaaggcg atggttcgaa cgggtgaaat cgagcgcacc
     6001 ctcgtccgat acgaggtgcg gaacggatgg gccaagaatt gcgcgaatcg attacaaacg
     6061 atgtaccgac ctgcccctat tcccatttct gcaacaattt caagatgtta catcacagat
     6121 gctgctggca tgctcagcga tacgatgcgg aaaaatccgg accgaaggtg cgcacacagg
     6181 cttttatctt tcgtgggttc gcggctcccc tgcttatcgc tgctcgacag gagcgcggtc
     6241 ttcatcaccg attatcactt cgcttccggt atcttcggcc gaaaactacc gtttcgcctt
     6301 ttttcggacg aagcgaattg atttcctttc gggataaccc gtttcattta tccattcgct
     6361 cggagcaaca ttggatcgag acgaatcgct gccgacaatt tttttgtgta tcggatgaac
     6421 aaccaaaggc atgtcaccat ccgcgcgccc gccgcgtcgt caaccacggg aaagaaacga
     6481 atgatgcgcc atggcaaaca atgacgatcg cgcgcttatc caacctcttc ctgaagagga
     6541 tatggactac attttggccc ggtgctccca ggagttcgaa accttgcggg gcgcgtcgat
     6601 cttcatcacg ggggcaagtg ggttcgtggg gagctggttg acgcaatcgc tgctgtgggc
     6661 gcaagaccga cgaaatctgg gccttcggct cgtgcttctc acccgctcgt cgacagcgtt
     6721 tgccgcccgt cagccagggg tagcccatca tccgtcggtt caccttctcg agggcgacat
     6781 gcaatcattc cc
//
LOCUS       DAOSOR010000308         6791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_11273_length_6791_cov_4.219121, whole genome shotgun sequence.
ACCESSION   DAOSOR010000308 DAOSOR010000000
VERSION     DAOSOR010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11273_length_6791_cov_4.219121"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="PK710_15575"
     CDS             complement(<1..1241)
                     /locus_tag="PK710_15575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOT11200.1"
                     /translation="MQERLQKILARAGVASRRAAERLIEARRVRVNGKIVSELGARAD
                     PRTDRVELDGKRIVAEDFVYVILHKPRAVMSTLDDPEQRPTVADILKPLPLRLFPIGR
                     LDYQTSGALFMTNDGDFAARLLHPRYHVPKTYLVKVQGVMTEQDIATWKQGVMLDDGI
                     TLPTEVVVTRRDDRFTWFELIMREGRNQQIRRMGEATGFPVIRLTRTAFAAITIDGLR
                     AGQWRFLTKDELRDLQGLYGLPKHLRAAVSTHARYANVQPILGRVAPRSARAPEPAPS
                     PRSFGRPAASRAAGDTVPTRRNASKPTNSETEPARNRPAYRHGPRDDSSQSQEPLRKP
                     KPDRKPTQKEATGRVEGPRREYSRKEGQFRAGKKDSESKTRPSKEKPREARPRPRADR
                     QENRQEKNKQDKPRAPEKRGGP"
     gene            complement(1256..1981)
                     /locus_tag="PK710_15580"
     CDS             complement(1256..1981)
                     /locus_tag="PK710_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11201.1"
                     /translation="MHAPSMNRAIPALLCAILASGCGSKTPAKDPSAADGDRSHGFSS
                     DDKARCEFGDNMDREVVESTTSGSNIPNIRRVYQAFGTGEDARRVLICREIDTNLDGV
                     KDVMRTYSDKGEAQREEADTNYDGRVDSWITFANGRIVKHEIDRNGDGKPDQWKYYVQ
                     GKLSRIQRDTNFDGNPDVWEIYVRGQLDRIGVDVDFDGRVDRWDRDEMSRLASETKKD
                     DSKSERSSPQEPTDKDASSTQHE"
     gene            complement(1993..2709)
                     /locus_tag="PK710_15585"
     CDS             complement(1993..2709)
                     /locus_tag="PK710_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11202.1"
                     /translation="MPPANYRYRFSRVVVLPLMAGCFAFGCASAVSKKPTEATADGRP
                     PVDIVHEPCDTDSKNATKTDANGDGRPDIIQVMNGNREVCRAIDLNFDGLIDNYLYFD
                     DAGNLRRRESDFDRDGLIDEIAYYQNGVIVQKHRETNLDGKLDTWDTYRQGLLTERLR
                     DSNRDGKVDQWWTFPDPNKLECPVVAVDESGTGRPDVRYDPCKEREAEMTASEPQHPP
                     TPAPDATPVDASTDDATGGT"
     gene            2910..3107
                     /locus_tag="PK710_15590"
     CDS             2910..3107
                     /locus_tag="PK710_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HOT11203.1"
                     /translation="MSRKKISTTIYVTPEQADRLKLLHERTKVPVAVYIREGIDLVLE
                     RYEHVLPGQMTLVTDGSGKER"
     gene            complement(3417..4604)
                     /locus_tag="PK710_15595"
     CDS             complement(3417..4604)
                     /locus_tag="PK710_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOT11204.1"
                     /translation="MGEMGYAFWPIPWLSPRHALVLVSLPMLALLQGLIAAALMSCLL
                     QNTTVRAAAPAWRPLVLTTLMPSAVLFFAIRWAGLPLASAAIDQAIWPVWAGFSCALL
                     LLGLASFSRRPLPPSWTRTLVGLGAALAMTCWSLAVAFALTPHRWLASPHFATALQPP
                     SLAPILAYHLASTFCFLLPFSDPLRDNARALLRALTVVAASFCSVVLLTASHWLPSET
                     VPAFSVDALERGNAALSTRWTMISTGAWLSVGIVSAFFSGTRARSAVLLGATIASIAS
                     QGTWMAYQEPWALRPQTYINGMQVMTARTQRRSPALPTDAPKRAEQVFDRQCASCHAK
                     QGPFSLRKTARRPKPESLRQRLETLREADRPWNPYHRVMPPLVGSDEEVNALGAWISN
                     EAN"
     gene            5042..6295
                     /locus_tag="PK710_15600"
     CDS             5042..6295
                     /locus_tag="PK710_15600"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOT11205.1"
                     /translation="MRREESDRKNRAATRPGVTIVFILAAALAPGCSSSDSDAQFGSG
                     GAGAGAGANGATGGSGGQTGIGSDGGPIFMDGSTEAEQPIKECAKSTAKASLLPTYLQ
                     FLVDVSGSMNCKPTDPPSKGCQPGDPGSKWMLTRSALGSAIDLLPVSSSAGAILFPDV
                     DPSGWNQLCFSNKSAVPMAPLTDGHKTLFKQVLNSTMAMGATPTEDAYLFAVQSFGAI
                     TEESNKFIVLITDGVPSVSKGCQGDGMTPVPTAPLVQAAAGAKAMGIKTFVIGSPGSE
                     AVRTPLSEMASQGGTATPGCSDSGPNYCHLDMTTQSNFGQAITDALLEVAGKTIACSF
                     ALPEEDGDAGKVDPNLVNVMYTPGGGEPELIGQNPQTPCTGVGWHYSDDKSHIILCEQ
                     TCKTVQADPQAEITIVLGCATEQVK"
     gene            complement(6374..>6791)
                     /locus_tag="PK710_15605"
     CDS             complement(6374..>6791)
                     /locus_tag="PK710_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11206.1"
                     /translation="LLLLASSVHVFLVHIHGLAPPRRPSRSIGWLEFFPRAALLWLLT
                     INVILIVAALCPAPFAAEPEPALPPRPAWCLMAFFYLAQHLRASTWLIVPTASIALLI
                     TIPWLDKLSARHRISWLVGIVLWAGWIVLSVLGYID"
BASE COUNT         1389 a         1718 c         2297 g         1387 t
ORIGIN      
        1 ggcccgccgc gtttttcagg cgctcggggc ttgtcttgct tgtttttttc ttgtcgattt
       61 tcctgtcgat ccgcccgtgg gcgcggacga gcctcgcggg gtttttcttt cgaaggccgg
      121 gtcttgcttt cactatcctt ttttccagcg cggaattgcc cttcttttcg gctgtattca
      181 cgacgcggtc cctcgaccct tccggttgct tccttttgcg tcggtttacg gtctggcttt
      241 ggcttgcgca gcggctcctg ggattgcgat gaatcgtctc gcggcccgtg gcggtaagcg
      301 ggacgatttc tcgctggttc cgtttccgaa ttcgtgggtt tggaagcatt gcgccgtgtg
      361 ggtacggtat cgcccgccgc tcgagaggca gcggggcggc caaaagaacg gggcgaaggg
      421 gctggctcgg gggcgcgggc ggagcgaggg gcaacacgtc cgaggatggg ctgcacgttg
      481 gcataacgcg catgggtgga gacggcagcg cgaaggtgct tgggcaggcc gtacaggccc
      541 tgcaagtcac gcaattcgtc tttggttaga aaccgccatt gaccggctct cagcccatcg
      601 atggtgatgg ccgcgaaggc cgtgcgggtg agacgaatca ccggaaaccc cgtggcttcc
      661 cccattcttc gtatctgttg gttgcgtccc tcacgcatga tgagttcgaa ccacgtgaag
      721 cgatcgtcgc gccgcgtgac cacgacttcg gtgggaagcg tgatgccgtc gtcgagcatc
      781 acgccctgtt tccaggtggc gatgtcttgt tcggtcatca caccctggac tttcaccaaa
      841 taggttttgg ggacgtgata acgagggtgg agcaggcgcg cggcgaagtc gccatcgttg
      901 gtcatgaaca gcgcaccgct ggtctggtaa tcgagccgtc cgatggggaa aagccgaagg
      961 ggaagaggtt tgaggatatc ggcgacggtg ggacgctgtt ccgggtcgtc gagcgtcgac
     1021 atgacggctc gaggtttgtg caggatgacg tagacgaagt cctcggcgac gatgcgtttt
     1081 ccgtcgagtt cgacacggtc ggtgcgagga tcggcgcgag cgccgagttc ggagacgatt
     1141 ttaccgttga cgcgaacgcg tcgggcttcg atcaagcgtt cggcggcgcg tcgagaagcg
     1201 acccccgcgc gcgcgaggat tttttgtaat ctttcttgca tggtgggagc gagggctact
     1261 cgtgttgcgt ggaggaggcg tctttgtcgg tgggctcttg tggggaggaa cgttcgcttt
     1321 tgctatcgtc ttttttggtt tcactggcga gccgactcat ttcgtcgcgg tcccatcggt
     1381 cgacacgtcc atcgaagtcg acatcgacac cgatgcggtc gagttgtccg cgtacgtaga
     1441 tttcccaaac gtcggggttg ccgtcgaagt tggtatctcg ttggattcgt gagagttttc
     1501 cttgtacgta gtacttccac tggtccggtt taccgtcgcc attgcggtcg atttcatgtt
     1561 tgacgattcg tccgttggcg aaggtaatcc aggaatcgac acggccgtcg tagttggtat
     1621 cggcttcttc tcgttgggct tcgcctttgt cgctgtaggt gcgcatgacg tctttgacgc
     1681 catcgaggtt ggtatcgatt tcgcggcaga tgaggactct gcgtgcatct tcgccggttc
     1741 cgaaagcttg gtagacgcgt cggatattgg ggatattcga accggaggtg gtggattcga
     1801 cgacctcacg atccatgttg tcgccgaatt cacatcgagc tttgtcatcg gaggagaagc
     1861 cgtgggagcg gtctccatcg gcggcggacg ggtctttggc gggggttttg gagccacagc
     1921 cggatgcgag gatagcgcac aacagggcag gaatggcgcg gttcatggaa ggggcatgca
     1981 tggtacgcat ccttacgttc ctccggtcgc gtcgtcggtg ctggcatcga caggggtagc
     2041 gtctggcgcc ggggttggag ggtgttgggg ctccgaggcg gtcatttccg cctctcgttc
     2101 tttgcagggg tcataccgta catcgggccg accggttccc gattcatcca ccgcgacaac
     2161 ggggcattca agtttgttgg gatcggggaa agtccaccat tgatcgactt tcccatcacg
     2221 gttggagtcc cgcagccgtt cggtaagcag accttggcgg taggtatccc acgtatccaa
     2281 cttgccatcg agattggttt cgcggtgctt ttggacgatc acaccatttt gataataagc
     2341 gatttcatcg atgagaccgt ctcggtcgaa gtccgattcc cggcgacgca agttcccggc
     2401 gtcgtcgaag tacaggtaat tgtcgatgag gccatcgaag ttgaggtcga tggcccgaca
     2461 gacttcgcga ttgccgttca tgacttgaat gatatccggg cgcccatctc cgttggcatc
     2521 ggttttggta gcgttttttg aatcggtatc acagggttcg tggacgatat cgacaggggg
     2581 acgaccatcc gcggtagcct cggtgggttt tttggaaacg gcggaagcgc atccaaaggc
     2641 gaagcacccg gccatgaggg gaaggacaac aacccttgaa aatcgataac gatagttggc
     2701 aggcggcatg acaagctccc ttgacgcacg gaacggggtc ctccggccca tgccccagag
     2761 tagtggcagt gtaagcgtgt gagagggtgc atgcaagcgg ggacatgggg ggagcccatg
     2821 gaaaggggtt ccgcgggaag gcagtgaaaa cattgacaac atacgtacct tcatctaggg
     2881 tattggctgg tcacaagctc gggcagccca tgtcacgaaa aaaaatatcc accaccatct
     2941 atgtcacgcc cgaacaggct gacagactca agctgcttca cgagcggacg aaagtcccgg
     3001 tcgcggtgta tatccgtgaa ggcatcgact tggtgctgga acgctatgag cacgtgcttc
     3061 cgggccagat gacgctcgtg acggacgggt cggggaaaga gcgataggtt gtgagggacg
     3121 gcttggcgcg agggtcgtag gccgccccgg gtcgaattcc tcccggagtg acaacggggc
     3181 gtttggcgcc gcttcatggg ggaaactatc tcccgcaaga cagcccgcgt gttgcacggg
     3241 agaggtggga gcgaatagta ttgctatccg tattgggata atatttgcga aatacctaat
     3301 gatttcaggc aattgctgtt gtccgtgtag gtatattcgg aaagcgattg ccatcgctat
     3361 ccccccgtcg ggcgtggtat ctccatgaag tgcatgggct cgatcccatc gatgccttag
     3421 ttggcttcgt tggaaatcca cgcgccaagc gcattcactt cctcgtccga gcccaccagc
     3481 ggcggcatga cccggtggta gggattccag ggacgatcgg cttcgcgtaa cgtttccagg
     3541 cgttgccgaa gtgattcggg cttggggcgt cgtgcggtct tgcgcagcga aaaaggtcct
     3601 tgttttgcgt ggcagcttgc gcattgccga tcgaatacct gctccgcgcg tttgggagcg
     3661 tcggtgggca acgcggggga gcgtcgttgt gtgcgagcgg tcatcacctg catgccgttg
     3721 atgtaggtct ggggtcgcag ggcccacggc tcttggtaag ccatccacgt gccttgggat
     3781 gcgatagagg cgatggttgc gccgagcagc acggccgatc gtgcgcgggt tccggaaaag
     3841 aaggcactca cgataccgac agaaagccag gcgccggtcg agatcatcgt ccagcgtgtc
     3901 gatagggctg catttccacg ctcgagggcg tcgacagaga aagcggggac cgtttccgag
     3961 ggtagccaat gggaagcggt gagcagaacg acgctacaga agcttgctgc tacgacggta
     4021 agggcgcgga gcaaggcgcg ggcattgtcg cgaagggggt cagagaatgg gagaagaaaa
     4081 cagaaagtcg acgccagatg gtaggccagg atcggtgcga gcgagggggg ttgcagggcg
     4141 gtggcgaaat ggggggaagc caaccatcga tgtggtgtca atgcaaaggc gacggcaagg
     4201 gaccagcagg tcatcgccag tgccgcgccc aagccaacga gcgtgcgggt ccaagaaggg
     4261 gggaggggac gacgcgaaaa gctcgccagg cccagcaaga gcagcgcgca cgaaaaccct
     4321 gcccagaccg gccagattgc ctgatcgatc gcggcggaag ccaggggcaa gccggcccat
     4381 cggatggcaa aaaaaagtac cgcgctgggc atcagggtgg tgagaacgag ggggcgccag
     4441 gcaggagccg cagcacggac agtcgtattt tgcagcaagc aagacatcag cgcggcggca
     4501 atcaatcctt gcagcagggc gagcatcggc aggctgacga gcaccagcgc gtggcgaggg
     4561 ctcagccatg gaatcggcca aaacgcgtag cccatctcac ccattatatc cagatacgga
     4621 tagttccgca gagcaggcaa aggcaaaagc agtcccaaac catggggttc gaggaagaag
     4681 ggaaagttcg ttccgccggt ggatgaagcg actcatttgg gtcgtggtcc tgcgtgtcag
     4741 ggttcgcttc tcgggtactt cccgtaagct ggaatgcaac tcatcgttgc agggggccgt
     4801 tttgccgttc atggtatcgt agacggaacg gatagcgaaa gaagggtgcg catgttggca
     4861 tccctgtgag aagggatcga gcgcggcgga aggctgcttc ggatccgccg ggttgggtca
     4921 agggagaggc tgtcctgtga attgatgtga aaatgaatgg gttggacaag caagatgcgg
     4981 cgggagcggt tggctcgcgg tatgctagga cgagcaagac gacgagcaag gaggaggagt
     5041 tatgagacgt gaagagtcgg atcgaaaaaa ccgtgccgcc acacggcctg gcgtgacgat
     5101 tgtgttcatc ctggccgcgg cgctggcacc agggtgctcg tcgagcgata gtgatgcgca
     5161 atttgggtcc ggaggagccg gggcaggggc gggagcgaat ggcgcgacgg ggggaagtgg
     5221 gggtcaaacg gggattggtt cggatggtgg acccattttt atggatggtt ctaccgaagc
     5281 ggagcagccg atcaaggaat gcgcgaaaag cacggcgaag gcgtcgctgt tgccgacgta
     5341 tttgcagttt ctcgtggacg tgagtgggtc aatgaattgc aagcccacgg atcccccctc
     5401 gaagggctgt caacccggcg atcctgggtc gaagtggatg ctcacgcgct cggctctcgg
     5461 aagcgccatc gatctattgc cggtatcgtc gagcgcaggt gccattctct ttcccgatgt
     5521 cgatccgagt ggttggaacc agctatgttt ttcgaataaa tccgctgtac cgatggctcc
     5581 cttgaccgat ggtcacaaga ccttgttcaa gcaggttttg aattccacga tggcgatggg
     5641 ggccacgccg acggaagacg cgtatttgtt cgccgtacag agtttcgggg ccattaccga
     5701 ggagtcgaat aagttcatcg tgttgatcac cgatggggtc ccatccgtat cgaaggggtg
     5761 ccaaggcgat gggatgacac cggtgcctac cgcgccgttg gtacaagctg ccgcgggcgc
     5821 aaaggcgatg gggatcaaga cattcgtgat tggatcgccg ggcagtgaag cggtgcgcac
     5881 gccgctatcg gaaatggcga gccagggagg aacggcgact ccagggtgct cggattccgg
     5941 cccgaactac tgccatctgg acatgaccac ccaatcgaat tttggtcagg cgatcacgga
     6001 tgcgcttttg gaagtagccg gaaaaacgat cgcctgttct ttcgcgctgc ccgaagaaga
     6061 tggtgatgcg ggcaaggtcg accccaacct ggtcaacgtc atgtatacgc ccggcggtgg
     6121 agagcccgaa ctcatcgggc agaatccgca gacgccctgc accggtgtgg gatggcatta
     6181 ttcagacgac aaatcacata ttattctatg tgagcagacc tgcaaaaccg tccaggcgga
     6241 tccgcaagcc gagataacca tcgtgctcgg gtgtgcgacg gaacaagtca agtagacgcg
     6301 tcatcacgga tttttcgact cgatcgcgag gcgaggacga cgggtatggc cacgatcgag
     6361 cccggatcaa gggtcaatca atatatccta atacggatag aacgatccag cctgcccaaa
     6421 gcacgatacc cacgagccag gagatgcgat ggcgagccga tagcttgtcc agccatggaa
     6481 tcgtgatcag gagggcgatg cttgcggtgg gtacgatgag ccaggtcgaa gcgcgaaggt
     6541 gctgagccaa gtagaaaaac gccatcaaac accaggcggg gcgaggcggt agggccggtt
     6601 cgggctcggc agcgaaaggt gcagggcaaa gggcggccac gatgaggatg acgttgatgg
     6661 tgagcagcca caatagggcg gctcggggaa agaactcgag ccagccgatg gagcgagaag
     6721 gccgtcgagg gggcgcaagg ccgtggatat gaacgagaaa aacatgcacc gacgaagcga
     6781 ggagcaaaag g
//
LOCUS       DAOSOR010000309         6789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_11279_length_6789_cov_4.471933, whole genome shotgun sequence.
ACCESSION   DAOSOR010000309 DAOSOR010000000
VERSION     DAOSOR010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11279_length_6789_cov_4.471933"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..755
                     /gene="gspM"
                     /locus_tag="PK710_15610"
     CDS             165..755
                     /gene="gspM"
                     /locus_tag="PK710_15610"
                     /inference="COORDINATES: protein motif:HMM:NF016493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HOT11207.1"
                     /translation="MKRLRQWFDGLAERERKLLLLLMAVFAIVIVLLVPFGISSMLSE
                     RREHNEALRKAIQTVQGSRGRLEEAKDKRAQIEHRYDKPAPALAGFIENAARKSGLEI
                     PESQDRAEVPHGKKFVERSTVVRLRKVSLLPLVQMLEAIENAGHPIVVSRLNLRRRGR
                     EADSYDVELGVSAYDRVAEKKGSKSTASTSSSGVKP"
     gene            752..1798
                     /gene="gspN"
                     /locus_tag="PK710_15615"
     CDS             752..1798
                     /gene="gspN"
                     /locus_tag="PK710_15615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HOT11208.1"
                     /translation="MSPRLRKILLWVMYPLFYVVCFVFFAYLTFPFDTLRDRVVLAFA
                     ENQRKTGGDSYLEIDKLESYWLSGAQARGVRLTSAPSVKSDGTKGKPTVFEVDRVAAR
                     VALLPLLIGRVTIHFSADLLGGTLRGSTRKSGEDRTIYLELSDLSVGEIEPIVELIGL
                     PLAGRLKGTVDLTLPEGKLAKATGSVDLVLQDLSVGDGKAKIKDTIALPRLVVGDLEL
                     TCDFSEGRVTITKFAASGNDLDFHAEGRLTLREKMMESQSDMYLRFRFADKYKSKDEK
                     TTALFGAPGSNAPALFEIADPRIRQSKRADGFYSWRASGLLRALRFDPAPAGGAGSGR
                     GQSSPPGGVRGFSR"
     gene            1881..2642
                     /locus_tag="PK710_15620"
     CDS             1881..2642
                     /locus_tag="PK710_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HOT11209.1"
                     /translation="MQQQPGSHGQAAPPGVGAAIVPAGSIAQQITHGPSFAMLRLDLA
                     PGQTIVAEAGSMVARHQSVTMEAKLNAPGGGAFAMLKALIIALIRKFVGGETFIVNHF
                     SAPQGGSVWLAPTMSGPVVYRRLQGETLVLSTGAYLAHCGDVRMGLKFGGLRGLLAKE
                     GAFFLEVTGHGDLWFTSYGGVEAVDVNGPFTVDNGHLVGYEGNLSFGIGTAGGGVMGM
                     VASGEGLVCKFNGQGRIYIQSRNTGSLVSWLSGMG"
     gene            2655..3326
                     /locus_tag="PK710_15625"
     CDS             2655..3326
                     /locus_tag="PK710_15625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HOT11210.1"
                     /translation="MQVELLYRPAHSLARVVLAPGESVVAESGAMVGMSPNVQMQTQS
                     TGGLAGGLKRLFGGESFFRNTFSATQGAGEVLFAQALCGDMELIDVPPQGLYIQNSAF
                     VFSTPNVEINTKVGGFKSFFSRAGMFVLHAMAQGPGQVGIGAFGGLQLISCDSNMVID
                     TGHLVAWDATLQYSVGKSASGWIGSFLSGEGLVCHFRGQGRIWMQSRNPAEYGQYVGG
                     MLPPA"
     gene            3342..4019
                     /locus_tag="PK710_15630"
     CDS             3342..4019
                     /locus_tag="PK710_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HOT11211.1"
                     /translation="MQTQLLQKPDFGMLKVTFEAAGEQMLVEASAMVAMDSGVQMKTQ
                     LQGGLLAAAKRKMLGGESLFQNTYTATAPMQRLYLAPAPEGDVEQTMVDGVTPVFLSS
                     GAFMACASSVTMDTRWEGARGFFSGAGLALLRAQGQGPLFFSSYGGIHAVDVTHGYIV
                     DTSHIVGFQGGLQYRVQKLGGLKSLFLSGEGLVCHFTGQGRLWLSTRNPGALASFLAP
                     FRPRKSN"
     gene            complement(4080..4346)
                     /locus_tag="PK710_15635"
     CDS             complement(4080..4346)
                     /locus_tag="PK710_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11212.1"
                     /translation="MNESLVLHWIDGGSAHIVQTDGHFATLQSTRQAAVGTPLVARVR
                     LESAERIRFKVKDCRREPNGFLLVGRWVDLSKTTREELLAITAA"
     gene            complement(4343..4852)
                     /gene="smpB"
                     /locus_tag="PK710_15640"
     CDS             complement(4343..4852)
                     /gene="smpB"
                     /locus_tag="PK710_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOT11213.1"
                     /translation="MLSPTFAMAKPSKKENLEPGMTLIATNRRASFDYELGERFEAGL
                     VLLGSEVKSLRSRAVGISEAWASIVQGEAYIEGMRISILDHAAYGHTQERRSRKLLLH
                     RKEILALEKAIDRGGMTVVATRLYFRNGKAKIELAIARGKRKTDKREAIKDRDAAREA
                     QILMREKNR"
     gene            complement(4903..6075)
                     /locus_tag="PK710_15645"
     CDS             complement(4903..6075)
                     /locus_tag="PK710_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11214.1"
                     /translation="MNHRLNLISLSFGLLSILGCGTESKDSTPYVSPDSGAAGAAGAA
                     GTAGASGAQDAAIDQGGSGGKEEEDATNFDVNPQDVTEPEGETILYAHDRDRLYTIDP
                     KDPQLTMTEVGLFDCIGEFGEPSMTDIAVDKEGKLVGVSSKALFLDMQIDGNVVRCSS
                     GKVPIVEGALGNNARFYGLTFAPPTTNLGGEETLIGANTIGELYIINRKTGELTIVGK
                     FGDVPANDGRGHDYPKEHVGKRWALSGDIVFLENKGSPIGFATLRDCLDPAEAPKNCS
                     TVDTLVEIDVNKLKPVTSGAVPLVTKSIRGQILPTGCKVESCGFGNIYGVAAFNDRVY
                     GFVYKRGEGSTPPSGMLIAINNDTGVAQLISSPLTGDGFAGAGVTTLAPVIPPPPK"
     gene            6218..>6789
                     /locus_tag="PK710_15650"
     CDS             6218..>6789
                     /locus_tag="PK710_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOT11215.1"
                     /translation="MNDVIERAKRWAQQDIDEETRGELLALVEQQNVSDLEDRMAGCL
                     DFGTAGLRGILGAGSNRMNRAVVARTTAGLAKHLLATFPDAASRGVVVGYDARRKSRE
                     FAEQAAQVLAGQGIVTHLFPSLATTPQTAFATIQLGAVAGVMVTASHNPPEYNGYKVY
                     WANGAQIIPPQDEQIVAAIEQTPPANEIPT"
BASE COUNT         1364 a         1696 c         2140 g         1589 t
ORIGIN      
        1 ctgggaacca ttcgttgttt cgagaacgtc aagatcgttc gaaccaacca agtggtgaac
       61 gagaatcgcc agaaatatgt cttggaattc gatctgaagt gtccggtgga gggggagaag
      121 aaaaaatcga gttcggaagg ctcggaaacg aaagaaggtg agccgtgaag cgccttcgac
      181 agtggtttga tggcctggcg gagcgggaac ggaagctatt actgctcttg atggcagtgt
      241 tcgcgatcgt catcgtcctg ctcgttccct tcggtatttc atcgatgttg tcggagcgaa
      301 gagagcataa cgaagcgttg cgaaaggcga ttcagactgt gcagggaagc cgcggacggc
      361 tcgaggaagc caaagacaag cgtgcgcaga tcgagcatcg gtacgacaaa ccagctcctg
      421 cgcttgccgg tttcatcgaa aacgcagcac gaaaaagcgg tttggagata cccgaatcgc
      481 aagaccgtgc cgaggtgccc catgggaaaa agttcgtgga gcgttccacc gtcgtgcgtc
      541 tgagaaaggt atcgctgctt cctctggtgc agatgctcga ggccatcgag aatgcggggc
      601 atccgatcgt cgtatctcgc ctgaatctcc gacggcgggg gcgagaggcg gattcgtacg
      661 atgtcgagct tggtgtatct gcttatgatc gggtggcgga gaagaagggg tccaagtcca
      721 cggcttctac gtcttcgagc ggggtgaaac catgagtcca cgtcttcgaa aaatcctgct
      781 ctgggtcatg tatccgctgt tttatgtcgt gtgtttcgtg ttctttgcgt atctaacgtt
      841 tcctttcgat actcttcgcg accgggtggt gcttgcgttt gcggagaatc aacgaaagac
      901 ggggggcgat tcctatctcg agatcgacaa gctcgagtct tattggctat cgggggcgca
      961 agctcgcggc gtgcgattga cttctgcgcc ttccgtcaaa agcgatggga cgaagggcaa
     1021 accgacggtg tttgaagtgg atcgggtggc ggcgcgggtg gcgttgcttc cgctgttgat
     1081 tggccgcgtg acgattcatt tttccgcgga tttgttggga ggaacgttgc gtgggagcac
     1141 gcgaaaaagc ggtgaggatc gaacgatcta tctcgagcta tccgacttgt cggtgggcga
     1201 gatcgagccc atcgtggagt tgattggact tcctcttgcg gggcgtttga aggggacggt
     1261 ggacctgacg ttaccggagg gcaagctagc gaaagccacg ggttcggtcg acctggtgct
     1321 gcaagattta tcggtggggg atgggaaagc gaagatcaaa gacaccattg cgcttccaag
     1381 gttggtggtg ggggacttgg agttgacgtg tgatttttcg gaagggcgtg tgacgatcac
     1441 gaagtttgcg gcgtcgggca acgacttgga tttccacgcg gaaggtcggt tgacgctgcg
     1501 agaaaaaatg atggaaagtc aatcagatat gtaccttcgg tttcgatttg cggacaagta
     1561 caagagcaag gacgaaaaga cgacggcgtt atttggggcg ccggggtcaa acgctcccgc
     1621 gttattcgaa atcgccgatc cgcggattcg acagtccaag cgggccgatg ggttttatag
     1681 ctggcgagcg tctggcttgc tgcgcgcttt gcgcttcgat ccggcacccg ccgggggggc
     1741 tggctcgggt cgagggcaat catcgccacc gggtggggtt cgcggatttt cccgttagat
     1801 tgcgttttct cacaagcacg ttgtggctca tggtatgagg ctccgtctta cggtgttgac
     1861 cgattcgacg gggagatatc atgcaacaac agccaggcag ccatgggcag gcggctccac
     1921 cgggagtcgg ggccgcgatt gttccagcgg gctcgatcgc ccaacaaatc acgcatgggc
     1981 cttcttttgc catgcttcgg ctcgatctcg cgccggggca gaccattgtg gccgaggccg
     2041 gttcgatggt ggcaaggcat caatcggtca ccatggaggc gaagctcaac gcgccggggg
     2101 gtggcgcgtt tgccatgctc aaagcattga tcatcgcttt gattcgcaaa ttcgtcggtg
     2161 gcgaaacctt catcgtcaat catttttcgg caccccaagg cggtagcgtc tggcttgcgc
     2221 caacgatgag cggccctgtg gtgtatcgtc gtcttcaggg ggaaacgctc gtgctatcca
     2281 cgggtgccta tcttgcgcat tgtggtgacg ttcgcatggg cttgaagttt gggggacttc
     2341 ggggcttgtt ggcgaaagaa ggagcgtttt ttctcgaggt taccggacat ggggaccttt
     2401 ggttcacgag ttatggaggt gtggaagcgg tcgatgtcaa cggtcctttc accgtcgata
     2461 acgggcattt ggtgggatat gagggaaacc tgtcgtttgg catcggcacg gccgggggag
     2521 gcgtgatggg aatggtggct tccggggaag gattggtttg caaattcaac gggcaggggc
     2581 ggatttacat tcaaagtcgc aacacgggct cgttggtatc gtggctttcg ggaatgggtt
     2641 gaggaggttc ggtcatgcaa gtggaactat tgtatcggcc agctcactcc ctggctcgtg
     2701 tggttcttgc tcctggagaa tcggtggtgg ccgagtcggg agcgatggtg gggatgtcgc
     2761 ccaacgtgca aatgcagaca caatccacgg gaggcttggc gggcggattg aagcgtttgt
     2821 ttggaggcga gtcgttcttt cgcaatacat tttccgccac gcaaggcgcg ggagaagtcc
     2881 tgttcgcgca agccttatgc ggggatatgg agcttattga tgtgcccccc cagggcttgt
     2941 acatccaaaa ctcggctttc gttttttcca cgccgaacgt ggagatcaac acgaaggtgg
     3001 gaggcttcaa gtcattcttt tcccgagcgg ggatgtttgt gttgcatgcg atggcgcaag
     3061 ggccgggcca ggtgggaatc ggtgcttttg gcgggcttca gctcatatcc tgcgatagca
     3121 acatggtgat cgacaccggg catctcgtgg cctgggatgc gacattgcag tatagcgtgg
     3181 gcaagagcgc atcgggttgg atcggctcgt ttttatctgg agaaggcctt gtctgtcact
     3241 tcaggggaca agggcgaatc tggatgcagt cgcgcaatcc agccgaatat ggacagtacg
     3301 tggggggcat gctgccgccg gcctagcaag gagaacgaaa aatgcagaca caattgctgc
     3361 agaagccaga cttcggaatg ctgaaagtca cgttcgaggc agccggtgag cagatgctcg
     3421 tggaagcttc cgccatggtt gccatggaca gcggcgttca aatgaaaacg cagttgcaag
     3481 gaggattgct tgcagcggcg aagcgaaaga tgcttggagg cgaaagcctt ttccagaata
     3541 cctacaccgc caccgccccg atgcagcgtc tgtatctcgc gcccgcgccc gaaggcgatg
     3601 tcgagcagac gatggtggat ggtgtgacac cggtatttct ttcctctggc gcgttcatgg
     3661 catgcgcaag tagcgtgacg atggacaccc gatgggaggg ggcgcgaggg tttttcagcg
     3721 gtgcgggact tgcgctactg cgggcgcaag gacaaggccc tttgttcttt tcttcctatg
     3781 gtgggattca tgccgtggat gtaacccatg gctatatcgt agacaccagt catatcgttg
     3841 gttttcaagg cggccttcag tatcgggtgc agaagcttgg aggattgaag agcctatttc
     3901 tcagtggaga aggcttggtt tgtcacttca cggggcaagg gcgcttatgg ctttcgacaa
     3961 gaaatcctgg cgcgctcgca agttttctcg cgccattccg acctcgaaaa tcgaattgag
     4021 cgagaagttg tccaccccac gactttggcg ctgatatccc tcgatctcgc tgcaatgatt
     4081 catgcagccg tgatggcgag cagttcttcg cgtgtcgttt tggataaatc cacccaccgg
     4141 cccacgagca aaaagccgtt gggttcgcga cggcaatcct tgactttgaa ccgaatacgt
     4201 tccgcggact cgagacggac gcgagcgacg aggggagttc cgacggcggc ctgtcgggtg
     4261 gattgcaacg tggcgaagtg gccgtcggtc tgtacgatat gggcgctgcc cccatcgatc
     4321 caatgtagca ccagggactc gttcatcgat tcttctcgcg catgaggatt tgcgcttcgc
     4381 gtgcggcgtc gcggtctttg atcgcttctc gcttgtccgt ttttcgtttt ccgcgggcaa
     4441 tcgcaagctc gattttcgcc tttccatttc ggaaatagag tcgggtggcc acgacggtca
     4501 tgccaccgcg gtcgatggct ttttcgaggg ccaggatttc cttacggtga agcaatagct
     4561 tgcggctgcg acgttcttgg gtatggccgt aggctgcgtg gtcgagaatc gagatgcgca
     4621 tgccttcgat gtaggcttct ccttgaacga tgctggccca ggcttcggat atgcccacgg
     4681 cgcggcttcg aagggatttg acctcactgc cgagcagaac gagtccagct tcgaacctct
     4741 cgcccagttc atagtcgaag cttgcgcgac gattggtggc gatgagcgtc atgcctggtt
     4801 cgagattttc ttttttgctg ggcttcgcca tcgcaaaggt gggcgatagc acgggacgag
     4861 cgttcgcggt agcgcggaaa taactattga atttcgctta ctttactttg gggggggagg
     4921 aatgacaggg gccagggtcg tgacgcccgc tcctgcaaaa ccatcacccg tcaaaggaga
     4981 gctgatgagt tgtgcgactc ccgtatcgtt gttgatggcg atgagcatcc cggaaggggg
     5041 ggtggagcct tcgccgcgtt tgtatacgaa tccgtataca cgatcattga aggccgcgac
     5101 cccatagatg tttccaaatc cgcaggattc gactttgcaa cccgtgggca ggatttgtcc
     5161 tcggatgctc ttcgtcacaa gaggcacagc gccggatgtc acgggcttga gcttgttgac
     5221 atcgatttcg accaatgtgt cgaccgtcga acagtttttg ggcgcttccg ccgggtcgag
     5281 acaatcgcgc aacgtcgcaa aaccgatggg ggagcctttg ttttcgagaa atacgatatc
     5341 gccggacaac gcccatcgct ttcccacatg ctctttcgga tagtcgtgtc cgcgtccatc
     5401 attggccggc acatcgccga acttgccgac gatggtaagc tcgcccgttt tgcggttgat
     5461 tatgtatagc tcgccgatcg tgttggcacc gatcaaggtt tcttccccac ccaagttggt
     5521 tgtcggagga gcaaacgtca aaccgtaaaa ccgcgcgtta ttgcccaaag ctccttcgac
     5581 gataggtact tttcctgacg agcatcgcac gacgttgccg tcgatttgca tgtccaagaa
     5641 caatgccttg gaagatacgc caacgagttt gccttctttg tcgacggcga tatccgtcat
     5701 cgatggttca ccaaattcgc cgatgcaatc aaacaggcca acttcggtca tcgtcagttg
     5761 cgggtctttg gggtcgatgg tgtaaagccg atcacggtca tgcgcgtaca ggatggtttc
     5821 gccttcgggc tcggtgacgt cttgggggtt gacgtcgaag ttggtggcat cctcttcttc
     5881 cttgcctccc gaaccacctt gatcgatggc cgcgtcttgt gcgcctgaag cacccgcggt
     5941 tccagcggct cccgcggccc ctgccgcgcc gctatcggga gagacgtagg gagtggaatc
     6001 tttggattcg gttccgcaac cgaggatcga aagcagccca aaacttagag aaattagatt
     6061 aagcctgtgg ttcattcatg caatttggca gaagctttgc gcctcgtcca cgccgattgt
     6121 ttggacattt catttgcgaa atgtcgttgg atgggcacgc ttggactgct gggggctttc
     6181 cgattcatta ggcctggact agataggctg ggtgacgatg aacgatgtca ttgaacgcgc
     6241 aaaacgttgg gcccaacagg atattgacga agaaaccagg ggcgaattgc ttgctttggt
     6301 ggagcagcaa aacgtttcgg atctcgagga tcggatggct ggctgtcttg atttcgggac
     6361 agcggggctg cgggggattt tgggagccgg ctccaaccgt atgaaccggg cggttgtggc
     6421 gcgaaccacc gctggccttg ccaaacacct gctcgccacg tttcccgatg ccgcctcccg
     6481 cggtgtcgtg gtcggctacg atgctcgtcg aaaaagccga gaattcgctg aacaagcggc
     6541 gcaagtcctc gccggacaag gcatcgtgac ccatcttttc ccctcccttg ccacgacccc
     6601 tcaaaccgcc tttgccacga ttcagcttgg agccgtggcg ggagtcatgg tgacggctag
     6661 tcataacccc ccggaatata acgggtataa ggtgtattgg gccaatgggg ctcagatcat
     6721 tccgcctcag gatgagcaaa ttgtcgcggc catcgaacaa acaccgccgg ccaatgaaat
     6781 ccctacctt
//
LOCUS       DAOSOR010000310         6783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_11289_length_6783_cov_3.960464, whole genome shotgun sequence.
ACCESSION   DAOSOR010000310 DAOSOR010000000
VERSION     DAOSOR010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11289_length_6783_cov_3.960464"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..827)
                     /locus_tag="PK710_15655"
     CDS             complement(126..827)
                     /locus_tag="PK710_15655"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOT11216.1"
                     /translation="MSQSSPSVDRRRAYRIQPRHAVAQCTTKRATGLYAVHDLSRGGV
                     ALTGMTPLSAGSKASLRLMIPGYQTMHVRGTVLRTLPQRSRENRVVIQFAPLEAKNED
                     NIGNLIVTELALGMFPRCLIASSSERELRWLSMGLQAIGVQTHGAKTPMEAISYLESS
                     PLRINAIIVGRKVGECDGVEFASFVAGAYPDLRRILANRPDDHRTHLARHVVHGSLEG
                     RWGVESLRQALFSKP"
     gene            complement(953..2212)
                     /locus_tag="PK710_15660"
     CDS             complement(953..2212)
                     /locus_tag="PK710_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11217.1"
                     /translation="MKSLESFRLPAWLLVGVVAVTLLATRANAAVRLEGPWPDADSAV
                     SLEAQGLTRSEAIRRLADAMGWSIVIHSPKTDVIDLVVHKQPAVNVLSMILADDDYVA
                     RREGSLIAIEKDVIKPTLIASSSSASPPSSAPPPDALPTASKPQDRVLTGGSTRIEKD
                     EVVRDVVVFGGSADVFGTATGDVTVIGGSARIHRGAHVMGDATAVGGSLHVDDGSRVD
                     GDVGVVGGVLRRGDTAQIGGRSASNSMGEKDEANEGNAWPWYRRVLTAAGRAIAEIAV
                     LFLLGSVFLALASSQMRELQAEVAARPMRSLALGIVGTICFAALLLALTISVVGIPIA
                     IVALLLVIMGAYAGVTSVLAVLGSALLGHRTRNPYTHLALGCLLFLLAKALPLLGTIV
                     LFVVVLLGFGTIVATRGASLLAKRSRV"
     gene            complement(2209..2721)
                     /locus_tag="PK710_15665"
     CDS             complement(2209..2721)
                     /locus_tag="PK710_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11218.1"
                     /translation="MSEPRMIPSDILWQKDGHLSDVALDALADGQDALVPLCVREHAN
                     ECEPCAIRLGDAFLLSAQAQTLLQQQSYRQIPWPLSLPSLLTVLVLALLGALPSLGGF
                     VAKAQNLAHTLSAVVPNLTRALVVLSRSEDMAHFIALVFLIAIVVLLAGAATIVRSLP
                     RQTASYGETR"
     gene            complement(2718..3317)
                     /locus_tag="PK710_15670"
     CDS             complement(2718..3317)
                     /locus_tag="PK710_15670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT11219.1"
                     /translation="MMPALMSPPCRPVPAVTAPRSFGEEVAEHIPIVRAVIASMLRER
                     RDHADVEDCVQEVLRRALEGYARLCPDKALRPWLLGIARHVALDHHRARQNTLRRVQN
                     ESPDESDSSSIIYDRIADPRPALDEQMDQLRRERQLRAAMNELTEGQRKAMLLFHVEG
                     LGYQDIAERLGVPLGTVATWISRGRRAIFTEIHAESRKP"
     gene            complement(3380..5116)
                     /locus_tag="PK710_15675"
     CDS             complement(3380..5116)
                     /locus_tag="PK710_15675"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT11220.1"
                     /translation="MSQSDSLPTPDVKGFLEISRVGIPGVQRQTLSGSHLILGRNPQA
                     QIVLDHTTVSRSHAELVFDPFGRWWVHDLRSTNGTYVNGSLVEERMLNPGDTIGIGDF
                     SLRLHIPGAREVRERADSFHPLPDAPAHLMADEDATLITVLPKITDSPQINASHLTTV
                     MALGRQLMSVEDPAKRLRTLCEFIVGSDFPADSVVTIRMRDGKPTKIVSGPFLRNPSI
                     PSPGRQVSIGVLKTLWDTREPVLGSNGMYSGHGIRRLSMPNAIRQLAVVACPLDMEDH
                     KLDALYVEFPPSYGTAEWLTLVALVAEAYQQAELVWDMRKHVRQSAFVERELQMARQI
                     QDGLVPRTLHIEGLDVAIGYEPCRWVGGDYVDIVPMPDGRILLAIADVCGKGLQAALV
                     ASSLHTMVHATIDAGGNLSQLIQSCNTYLCSYLPEHSFVTMMLVAIDTMSGELEVVNA
                     GHPPAFAVSPDGRVRQLQSERNVALGMMETRMEMERSMLIGNELLVLYSDGLTEMVDE
                     NQVALGEERLSAHLADIVQREPHATVEEYRERVLQRLTAYRGSQLATDDSTFLVARRP
                     YFPRHGGRMYEI"
     gene            5470..6444
                     /gene="nadE"
                     /locus_tag="PK710_15680"
     CDS             5470..6444
                     /gene="nadE"
                     /locus_tag="PK710_15680"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOT11221.1"
                     /translation="MFSRDVLKIDAAAVAETIQAQIREQVLGTLRRKGAVVGLSGGID
                     SSVVAALCARALGKERVLGLFMPEHHSSDDSLMLGQMLVSAIGIDAQIENLGPTLEAA
                     GCYRRQEEAIRTVFPEYGPGFKSKITLPSILEGNRLNVFQLTIQTPQGEIKSSRMKPA
                     AYLQLVAATNFKQRLRKMMEYYHADRLNYAVAGTPNRLEYDQGFFVKQGDGAADFKPI
                     AHLYKTQVFALAEFLGVPEEIRRRTPTTDTYSMAQTQEEFYFALPYDRMDLCLYGHNH
                     GVAPAEVAPVVDLLPDQVERVYKDIEAKRRTTQYLHLRPLLVEPVNEV"
     gene            6447..>6783
                     /locus_tag="PK710_15685"
     CDS             6447..>6783
                     /locus_tag="PK710_15685"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HOT11222.1"
                     /translation="MCGIAGILNLRGRQDPAATLDQLGRMAATMRHRGPDEVGVYRDA
                     SIGLAHSRLSITDLATGQQPLGNEDASLWVVFNGEIYNFIELREQLKALGHVFRTQSD
                     SEVIVHAYEQ"
BASE COUNT         1566 a         1891 c         1940 g         1386 t
ORIGIN      
        1 gcgtgacgag ggtgagcagc ccagcgccaa agatcgataa gatactgtgt tccatgggcg
       61 ggtaatggtg ttcgttggag cccgacgacc accaaagggt tcacggcaaa gcatccggac
      121 cgttttcacg gtttggaaaa aagggcctgt cgtaagcttt ctacccccca gcgcccttcc
      181 aaactgccat gaaccacgtg gcgcgcgaga tgagttcgat gatcatccgg acggtttgcc
      241 aaaattctgc gcaaatccgg ataagctccg gcaacgaaag aagcaaactc gaccccatca
      301 cactcgccaa ccttacggcc cacgatgatg gcgttgattc gcagcggcga tgattccaga
      361 tagctgatcg cttccatcgg cgttttcgcc ccatgggtct gcaccccaat ggcttgaaga
      421 cccatcgaca accagcgaag ttctcgctcc gaagacgaag caatcaagca tcggggaaac
      481 atgcccagcg ccagttccgt tacgatgagg ttgccaatat tatcctcgtt cttcgcttcg
      541 agcggagcga attggatgac cacgcgattc tcgcggctcc gctgtggaag cgttcgcaat
      601 accgtgcccc gtacatgcat cgtttgatag ccgggaatca tcaaccgaag agaagctttc
      661 gaccccgccg acaacggagt catcccggtc aacgccacgc ctccccgcga taaatcatgt
      721 accgcataca atccggtcgc tcgcttcgtc gtgcattgcg caacagcatg acgaggctga
      781 atacgatagg cgcgtcgacg atccaccgat gggcttgact ggctcatggg aacctcctgg
      841 acagcgaggt ctgaaacgcc acacgaccca ccgtcatggc gcgaagtgcg cgtcccatac
      901 ccttgtcttt gagcgatgcc cccatgaccg caagcctttc gacgactctt cttcagactc
      961 gtgaacgctt cgctagcaag ctggcaccac gggtggcaac gatggttcca aacccaagca
     1021 atacaactac gaatagaacg atggttccca acaagggcag cgctttggca agcaaaaaca
     1081 gcaaacaccc cagcgcgaga tgggtgtacg gattgcgtgt tcgatgaccg agcaaggcgc
     1141 ttcccagcac agccagaaca ctcgtcacac ccgcgtacgc ccccatgatg accagcaaca
     1201 aagcaacaat cgcgatggga atgcctacca cgctgatggt gagggcaagt agcaatgcgg
     1261 caaaacagat cgtaccgacg ataccaagcg ccagcgaacg catgggacga gcagccactt
     1321 ccgcttgtag ctcgcgcatt tgcgaagaag caagcgccag aaacacggat ccaagcaaga
     1381 acaaaacagc gatttccgca atggctcttc ccgcggctgt gagcactcgc cgataccaag
     1441 gccatgcatt gccctcattc gcctcgtcct tctcgcccat ggaattggag gcggaacgtc
     1501 cgccaatctg cgcggtatcg ccccttcgca acacgccacc tacgacaccc acatcaccgt
     1561 cgacacgcga gccatcatcc acatgcaaag acccacccac ggccgttgca tctcccatca
     1621 cgtgtgcgcc acgatgaatt ctcgccgacc ctccaatgac cgtaacatcg cccgtcgcgg
     1681 tgccaaacac atctgcagag cctccgaaca ccaccacgtc gcggacgact tcatcttttt
     1741 cgatccgtgt gctgccacca gtcaatacgc gatcttgcgg cttgctggcc gtgggtagag
     1801 cgtcgggggg cggcgcggaa gaaggggggg aagccgacga cgatgaagct ataagtgttg
     1861 gttttataac atctttttct attgctatga ggctcccttc tcgccttgcc acatagtcgt
     1921 cgtccgccaa aatcatcgac agaacgttga cggcaggctg cttgtgaacg accagatcga
     1981 tgacatcggt tttcggagaa tgaatgacga tactccaccc catcgcatcg gctaaccgcc
     2041 gaatggcttc cgaacgagta agcccttggg cctctagaga aacggcagaa tccgcatcgg
     2101 gccaagggcc ttccaaacgc acggcagcgt tggctcgtgt ggcgagcagc gtaaccgcta
     2161 ccacccctac gagaagccat gcgggcagac gaaaggattc gagggatttc atcgcgtttc
     2221 tccgtaggag gcggtttgtc ggggaaggga acggacgatc gtggcggcac cggcaagcag
     2281 aacgacgatg gcgatcaaaa agaccaatgc gatgaaatgg gccatgtcct cggaacggga
     2341 taacacaacc aaagcgcgcg tcaggttcgg aacgacggcc gagagcgtat gagccaggtt
     2401 ttgtgctttg gcgacaaatc ccccaaggga aggcaatgcg ccgagcaacg ccagaaccaa
     2461 cacggtcagc aacgacggaa gggaaagagg ccaggggatt tgccgatacg attgctgctg
     2521 caacaaggtc tgtgcttgtg cggataacag aaaagcatct cccagacgga tcgcgcacgg
     2581 ctcgcattcg ttcgcatgct cacgaacgca aaggggaacg agcgcatcct gtccatcggc
     2641 cagcgcatcg agcgcaacat ccgaaagatg accatccttt tgccaaagga tgtccgaggg
     2701 aatcatccta ggttcgctca tggtttcctc gattcggcat gtatttcggt aaaaatggct
     2761 cttcgtccac gagatatcca cgtggccacc gtgccaagag gaactccgag gcgctcggca
     2821 atatcctgat agccaaggcc ttccacgtga aacagcagca tggcttttcg ttgtccttcg
     2881 gtcagttcgt tcatggccgc gcgcaactgt cgttcgcgac gcaattgatc catctgttca
     2941 tcgagcgctg ggcgggggtc ggcgatccgg tcgtagatga tcgaggacga atcgctttca
     3001 tcgggggatt cgttctgcac acgccgcaag gtattttgtc gggcacgatg gtggtctagg
     3061 gctacgtgtc gcgcgattcc cagaagccat ggacgaaggg ctttgtcggg gcatagtcgg
     3121 gcgtatcctt caagggcgcg gcgtaagact tcttgaacac aatcctccac atccgcgtga
     3181 tcgcgtcgtt cacgaagcat cgaagcgatg accgcgcgta cgatgggaat atgctcagcc
     3241 acctcctcgc cgaaggaacg aggtgccgtg acagcaggca cgggacggca agggggggac
     3301 atgagggccg gcatcattgt gcgccggatc cttcgttttg gcagaccaga ttatttcatc
     3361 gggatagcct ggtccccatt caaatctcgt acatacgccc accgtgtcgc gggaagtagg
     3421 gacgcctcgc caccaaaaac gtgctgtcat cggtggcaag ttgcgaacct cgataggcgg
     3481 tgagccgctg gaggactcgt tcgcggtatt cctcgacggt ggcgtggggt tcacgttgaa
     3541 cgatatcggc caggtgggcg ctaaggcgct cttcaccgag agcgacttgg ttttcgtcca
     3601 ccatttccgt caggccatcg gaataaagga ccaacaattc attgccgatg agcatggatc
     3661 gttccatttc catgcgtgtt tccatcattc ccaacgcaac atttcgttcg gattgaagct
     3721 gtcgaacgcg tccatcggga ctgacggcga aagcgggtgg atgaccggcg ttgacgactt
     3781 cgagttcgcc gctcatcgta tcgatggcaa cgagcatcat cgtcacgaag gagtgttcgg
     3841 gcagatagct acacaggtag gtgttgcagc tttggatgag ttgggaaagg tttccgcccg
     3901 cgtcgatggt ggcatgcacc atcgtatgca aactggaagc gaccaacgcg gcttgcaacc
     3961 ctttgccgca aacgtcggcg atcgcgagca gaatacgccc gtcgggcatc ggaacgatat
     4021 cgacataatc accaccgacc caacggcatg gttcgtatcc gatggcgaca tccaagcctt
     4081 cgatatgcag agttcgcggc accagcccat cttggatttg cctggccatc tgcaattcac
     4141 gttcgacgaa cgcggattgt cggacgtgtt ttcgcatgtc ccaaacgagt tccgcttgtt
     4201 gatacgcttc agccacgagt gcaacgaggg tgagccactc ggcggtaccg taagaggggg
     4261 gaaactcgac atatagggcg tcgagtttat ggtcctccat gtcgagaggg catgcgacaa
     4321 ccgcgagttg gcggatggca ttgggcatgg aaaggcggcg tattccgtgt ccggagtaca
     4381 ttccattgct tcccagaacg ggttctcggg tatcccacaa ggttttgagc acaccgatgg
     4441 aaacctgtcg tcctggggag gggatagaag gattgcgcag gaaaggtccg cttacgattt
     4501 tcgtgggttt tccatcgcgc atgcggatgg tgacgaccga atcggcgggg aagtccgaac
     4561 ccacgataaa ttcgcagagg gtacgaagtc gtttcgcggg gtcttcgacg ctcatgagtt
     4621 gtcgaccgag cgccatgacg gtggtcaaat ggctcgcgtt gatctgcggg gaatcggtaa
     4681 ttttaggcaa tacggtgatg agggtggcat cctcatcggc catgaggtga gccggagcgt
     4741 ctggcaacgg atggaaggag tcggcgcgct ctcggacttc tcttgcgcct gggatatgga
     4801 gacgaaggga gaagtcaccg atgccgatgg tatctccggg gttgagcatt cgttcttcga
     4861 ccagggagcc gttgacatac gtgccgttgg tgcttcgcag atcatgaacc caccagcgac
     4921 cgaacggatc gaaaacgagt tcagcatgcg agcgggatac ggtggtgtgg tcaagaacga
     4981 tttgggcttg tggattgcgg ccaagaatga ggtgagaacc ggataaggtc tgtcgctgga
     5041 caccggggat gccgacgcga gagatttcga gaaatccttt gacgtcgggg gtaggcaggc
     5101 tatcgctttg agacatacgt aattcgacgt gtagacgatt ctggaggaaa aatctcgatc
     5161 ttccttatcc gtcggctcga ctcgggagga aacagtcagc caggacggtt gcgtcgggga
     5221 cgaacgctct tatatctgaa cggtcctttc caaccgtcct gatcgtggcg ttgttcacgc
     5281 aacggttcgc gacggagtgg aaattgatgt gccaataacg atcaaacgac tcaaatgaat
     5341 ggattaagag cctctatcca gccttccgtt ggcaccaagg acgtgctacg cgacaaggtt
     5401 cgatatggca aaagtcgcag tcgagtcgct gcggcttgag gttttccaac cgagcagcaa
     5461 ggggtgatta tgttctctcg cgacgtactg aagatcgatg cggcggcggt agcggagacg
     5521 atccaggcgc aaatccgaga acaggttctt ggaacgttgc gacgcaaagg agctgtcgtt
     5581 ggcttgtctg gaggtatcga tagttccgtg gttgcggcgt tatgcgccag ggcccttggc
     5641 aaagagcggg tgttgggtct gttcatgcct gaacatcatt cttccgacga ctcgttgatg
     5701 ctgggtcaaa tgctggtttc ggcgatcggc atcgacgcgc agatcgaaaa tcttggaccg
     5761 accctcgagg cggctggttg ttatcgtcgt caggaagaag cgattcggac ggtatttccc
     5821 gagtatggtc ctggattcaa gagcaagatc acgttgcctt cgattctcga gggcaatcgg
     5881 ctcaacgtat ttcaactcac cattcaaacg ccgcaagggg aaatcaagtc ttcacgcatg
     5941 aagcccgcgg cctacctgca attggttgcc gccaccaact tcaaacagcg attgcgaaaa
     6001 atgatggagt actatcacgc cgaccggctg aattacgccg tggcgggcac ccccaaccgt
     6061 ctcgaatacg atcaaggatt tttcgtcaag caaggcgatg gcgccgcgga cttcaaaccc
     6121 atcgctcacc tgtacaaaac acaggtcttc gcccttgcgg agttcctcgg cgtgcccgag
     6181 gagatccgtc gtcgcacccc aaccacggac acatactcca tggcgcaaac ccaagaagaa
     6241 ttctatttcg cccttcccta cgaccgcatg gacttgtgtt tatacggcca caatcacggc
     6301 gttgctccag cggaagtggc accggtcgta gacctgctac ccgaccaagt cgaacgtgtc
     6361 tacaaggata tcgaggcgaa acgtcgcacc acccaatacc tacacttgcg cccattgctt
     6421 gtggagccgg tcaacgaggt ttgagcatgt gcggcatcgc cggtattttg aatcttcgtg
     6481 gacgacagga tcccgcggca acactcgacc aactcggtcg gatggctgca accatgcggc
     6541 atcgaggtcc cgacgaggtt ggcgtttatc gtgatgcaag catcgggctc gcccattcac
     6601 gcttatccat cacggacctg gccaccggtc agcagccatt gggcaacgag gatgcttcct
     6661 tatgggtcgt gttcaacggt gaaatctaca atttcatcga attacgagag caactcaagg
     6721 cccttggtca tgtgtttcga acgcaaagcg attcggaagt catcgttcat gcgtatgagc
     6781 agt
//
LOCUS       DAOSOR010000311         6767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_11333_length_6767_cov_4.879023, whole genome shotgun sequence.
ACCESSION   DAOSOR010000311 DAOSOR010000000
VERSION     DAOSOR010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6767
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11333_length_6767_cov_4.879023"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PK710_15690"
     CDS             <1..384
                     /locus_tag="PK710_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11223.1"
                     /translation="GKKVSGNVSYAALQFFADWYVDPESGLHFEGALGTAGIIYDPTP
                     SRRTDREGDEVTLGGFGGSLGVGWEGWVGEEWSLGGLLRLNWAWIDGVLDPSAPVYLL
                     RESGNDDNSTIFAVAPMLMFTATLH"
     gene            complement(401..1207)
                     /locus_tag="PK710_15695"
     CDS             complement(401..1207)
                     /locus_tag="PK710_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT11224.1"
                     /translation="MTLRIGIISDLHFGPRILFHGRLRKMSDMAPELTVAFVQRMNEL
                     KPDLVLTLGDIIEDESLETDANRYRHALRLLSEINAPVRHVCGNHDTINQTDDMIREA
                     WNHQGELYYSFDCKGYHVSVLRTVERPHIDARLPSEQLEWLRKDLEQTTLPTVVAMHH
                     GAADQNLRNNPWFENAPEVALIKERKALRSVISSSPRVRLVLNGHLHWTHVGIHDAIP
                     YVTVQSLTENLDPDAPGRVAAAWALVELSDHHVLVRTEGAAPSVFESSLP"
     gene            1318..1671
                     /locus_tag="PK710_15700"
     CDS             1318..1671
                     /locus_tag="PK710_15700"
                     /inference="COORDINATES: protein motif:HMM:NF017922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HOT11225.1"
                     /translation="MASKIPHPVNIKAPHGATVMHIEWSDGHKSVYPHDILRGYCPCA
                     DCQGHSGEIKFRQGGNTEIRDIATVGNYAITITWGDRHNTGIYNFEYLRMLCQCPICK
                     PPESPAVPSPDHHEK"
     gene            1745..2218
                     /gene="greA"
                     /locus_tag="PK710_15705"
     CDS             1745..2218
                     /gene="greA"
                     /locus_tag="PK710_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOT11226.1"
                     /translation="MERVPITPDGIAKLREELKQLRDVERPRISKAIGEAIAMGDLSE
                     NAEYHAAKERQGMVEARIKELEDKISRAEVIDPSKLSGSRVAFGATVTVFDIEADKEL
                     TYQIVGADESDVDQNRISVTSPIARALMGKECGDEIRVRTPGGQRVLEIVDVLYR"
     gene            2247..5309
                     /locus_tag="PK710_15710"
     CDS             2247..5309
                     /locus_tag="PK710_15710"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT11227.1"
                     /translation="MSHSDSVPVPTRKTWLTRFWQELGAGVLAVFVVLACELVGIALY
                     YHSRLTGPWELRAALLGLLPPALLLSLPLVLLSVGAARILEDERRFGRIIVAVGGACA
                     AAALGYGVTFGRHFQSIAVRMGFIVGIVMVSIAVCWWLLPSVIRIRSRSPRVFVLAAL
                     IGSVGIELANFLVLPRLYPVFHTALTALAVMMVALAGTVLAGSLSTRRSLVAASCLVI
                     LAAAITPFSRSALLSWDNVRLLYVNHAPTFAQVLSVMAASGAAEPSIDEVDIVEQRAE
                     RWEDWHERDVLLLTVDALRADRLGVYGYERRTSPRIDALAASGIVFERAYTVMPHTSY
                     AMTSLLTGKYIRPLLIRGTGQDSDTLAAVLRTYGYRTAAFYPPSLFAVDEERFAWAKA
                     SGLDFEYRKLEYAEAGLRRDQVMAYLDFEVGDHRFFVWAHFFEPHEPYAPPEGFDFGP
                     RATDRYDGEIAAVDAVIGQLVQAVWERRPDTVVVITADHGEAFGEHGAYYHGTTVYEE
                     QVRVPLIVLAKGLQARRVSVPVQLIDVMPTILHAMGIPVRPRVRGIDLGGWMSGNGQG
                     DGFAFSETHDQVLLAEGKWRLVCERRVDACSLFDLSVDSQQLHDCSAQESEVFIRLRQ
                     RLRAFESSHGAYERTASRAEGKDLPEALVRGMAGDAEAAEEVAGLLEDADVVLRRKAA
                     EVLFQLARKESAPSLSLAISRDEDGEVRQWCALALTRLGHGAPLTLELLRGGDEKWRR
                     LAALVLAESGEPQGAGVLLAWWETQDPSIEQRKQIAHALGKIGWKAAVVPLCKWLDHE
                     QLRPILAEALARLHDPYAKIVLLRYFSQERYLHTRLILARSLVALGASREMAPALMRF
                     LGVADPLEGGVGIAREAKILDALGGPNEKSLAEISKAGSEGVDVSVIVPKGGNGKGYR
                     AIVRVRSKQAGRVQLSRGDRGGRSSTMASNSAVVFDVPAGLSEVHAELGDAFGAMGSG
                     PLHVVLVPSPGVQVEGFVIVPLADEIPPPAPEPWVPDTLGDGSVESMK"
     gene            5500..>6767
                     /locus_tag="PK710_15715"
     CDS             5500..>6767
                     /locus_tag="PK710_15715"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT11228.1"
                     /translation="MRRLASRRGADLYLGQHAGPMGFQRDCVLKLVPSPAASGDSRLA
                     EELAHEASICSTLHHPAVVRMYDFFEHGDNLVLVFEHFAGVSLARLLSHFRRRRQRVP
                     DAAVWYLSHRLFGALAHAHALLDEKGKRTPVIHRDVQPANLIVSHDANVRLAGFGIAK
                     IAGTPATTAVGFVKGTPAYMAPEQAAGEKVTERVDVYAAGLVVWEMLSGRSVRPPGDI
                     GQGAELLRLIAGRRFEPIASLRGDIPREIAAAVDACLIPAAERRTIRCREVERWLEKV
                     VDVEAGKKELRERLVQLRNANVRTTAPRVSAAPRGSQPPHPLPSSQPRAFPSRFPGLA
                     TRVTGRPSEREEGEEPRSSRRVPSFRASSYPAPPLTSEKDTADAEPKKKSLLGLAPAL
                     PAFTSPMPDRVEPARTSYDAVAPPPMDVEA"
BASE COUNT         1282 a         1550 c         2286 g         1649 t
ORIGIN      
        1 gggaaaaaag tgtcagggaa cgtgtcgtat gcggcgttac agttttttgc ggattggtat
       61 gtggacccgg agtctggctt gcacttcgaa ggagccctgg gcacggcggg tatcatctat
      121 gatccaactc cctcgagaag aaccgaccgg gagggagatg aagttacatt gggtgggttt
      181 ggaggttcgt tgggagtcgg ttgggaagga tgggtggggg aggaatggtc cttgggaggc
      241 ttgttacggc tgaactgggc gtggattgat ggggtgttgg acccttccgc gcccgtttat
      301 ctccttcggg agtccgggaa tgatgataat tcgacgatct tcgcggttgc gccgatgcta
      361 atgttcaccg cgacgcttca ttgatgatcc cagtgtaacg ttacggcaaa gagctttcga
      421 atacgctggg ggcagcgcct tcggtacgga ccaatacgtg atgatcggat aattccacca
      481 gggcccaagc ggcggccacg cgccccggcg catcgggatc caagttttcc gtcaggcttt
      541 ggactgtgac atatgggatg gcatcgtgaa ttcccacatg agtccaatga agatgtccgt
      601 tgagtacgag acgtacccgt gggctcgatg aaatgacgga acgcagtgct tttctttctt
      661 tgatgagagc cacttccgga gcgttttcga accaggggtt gtttcgaaga ttctgatcgg
      721 ccgcgccatg atgcatggcc acgaccgtgg gcaaggtcgt ttgttcgaga tctttgcgca
      781 accactcgag ttgttcggaa ggaaggcgag catcgatgtg gggccgttcg accgttcgca
      841 gcaccgaaac gtggtatccc ttgcagtcga agctgtaata cagctctcct tgatgattcc
      901 aagcctcacg aatcatatcg tcggtttggt tgatggtatc gtgattgccg cagacgtgtc
      961 ggacgggggc attgatttcg gataacagac gaagcgcatg acgatatcgg ttcgcgtctg
     1021 tttcgaggga ttcgtcttcg atgatatcac ccagggtcag aaccaggtcg ggtttgagtt
     1081 cgttcattcg ctgaacgaat gcgacggtga gttctggtgc catatcgctc attttgcgca
     1141 ggcgaccgtg aaataggatc cgggggccga agtgaagatc agaaatgata ccgatgcgca
     1201 gagtcatggg cgagccatag cgcgttgtat tctgaagggc cattggggaa atcgagacaa
     1261 cgaaaggctt cgcttcggtc ttgcaaactc cgtgtaaagg accatgattc gtccgcgatg
     1321 gcgtcgaaga ttcctcatcc cgttaacatc aaggctcccc acggggccac cgtcatgcat
     1381 atcgaatggt ccgatgggca caaatccgtt tatccgcatg atattttgcg cggatactgc
     1441 ccttgcgcgg attgtcaggg gcattccggg gagatcaaat ttcggcaagg gggcaatacg
     1501 gagattcggg acatagcgac ggtcggcaac tatgcgatca cgataacgtg gggggatcgg
     1561 cataacacgg gaatctacaa cttcgagtat cttcgaatgc tgtgtcagtg cccgatttgc
     1621 aaacctcccg aatctccagc cgtaccgtcc cctgatcatc acgaaaaatg aaagcaaagg
     1681 tggcatcgtg tcgaattgag ttgtgacgat gatgcgccac gattgcgcca ggaggagtcg
     1741 aaggatggaa cgagtcccga taacgcccga tgggatcgcc aaattgcgtg aggaactcaa
     1801 acaattacga gatgtcgagc gacctcgtat cagcaaggcc attggcgagg cgattgcgat
     1861 gggcgatcta tctgagaacg cggaatacca cgcggccaaa gagcgtcaag gcatggtaga
     1921 agcgcgaatc aaggaattgg aggataagat ctcgcgcgcg gaggtcatcg atccttcgaa
     1981 attgagtggg tcacgggtcg cattcggtgc gacggtgacg gttttcgaca tcgaggcgga
     2041 caaggagttg acgtaccaaa tcgtgggagc ggatgaatcc gatgtcgatc agaatcgaat
     2101 ttcagtgact tcgccgattg cgcgtgcgct catgggcaaa gaatgcggag atgaaatccg
     2161 ggttcgaacc cccggtgggc agcgcgttct cgagattgtt gacgtccttt atcgatagcc
     2221 cctctccgtt tgtcgttcac gatacgatgt cccattcaga ttccgttccc gtgccgacac
     2281 gaaaaacgtg gctcactcgt ttttggcaag agttgggggc aggggtcctt gcggttttcg
     2341 tggtacttgc gtgcgagttg gtggggattg ccctctatta ccattcccgt ctgacggggc
     2401 cttgggagct tcgtgcggcg ttgctggggt tgctgccccc agcgttattg ctttctctgc
     2461 cgcttgtatt gttgtcggtg ggagccgcgc ggattctgga agatgagcga cgttttggtc
     2521 gaatcatcgt tgccgttggg ggtgcatgtg cagccgctgc cctgggctat ggcgttacct
     2581 ttggtcggca ttttcagtcg atcgcggttc gaatgggttt catcgtcggg atcgttatgg
     2641 tttccatcgc ggtctgttgg tggttgttgc cttcggtgat tcgcattcga agccgttcgc
     2701 cccgtgtgtt cgtgcttgcc gcgctcatcg gatcggtggg gatcgagctg gcaaatttcc
     2761 ttgtacttcc acggctttat ccagtgtttc atacggcgct gaccgcgctg gcggtgatga
     2821 tggttgcgct ggcggggacc gtgttagccg gttcgttgag tacaagacga tcgctggtgg
     2881 ctgcatcttg cctggtgata ctcgcggctg ccatcactcc gttttcgcgt tccgcgctgc
     2941 tttcatggga taatgttcgg cttctttacg tcaaccatgc ccctaccttt gcgcaagtgc
     3001 tgtcggtgat ggcggcaagc ggagcggcgg aaccttcgat cgacgaagtt gatatcgttg
     3061 agcaaagagc ggagcgttgg gaggattggc atgaacgtga cgtgttgctt ctcaccgtgg
     3121 atgcgctacg tgcggaccgg ttgggagtgt atgggtacga acgtcgaaca tcgccacgga
     3181 tcgacgctct tgccgcgtcc gggattgtgt tcgagcgcgc ctatacggtg atgccgcata
     3241 catcttacgc catgacttcg ttgctcacgg gcaagtacat tcgacctttg ttgattcgtg
     3301 ggactgggca agattcggat acgcttgcgg cggttcttcg cacctatggg tatcggacgg
     3361 cggcgtttta tcctccctcg ttatttgcgg tcgacgaaga aagatttgcg tgggccaaag
     3421 cttccggatt ggatttcgaa tatcgtaaac tcgagtatgc cgaagccgga ttgcgtcgtg
     3481 atcaggtcat ggcgtatttg gatttcgagg ttggggacca tcgatttttt gtgtgggcgc
     3541 atttttttga acctcatgag ccttatgcac cgccggaagg gttcgatttt ggtcctcgcg
     3601 ccaccgatcg atatgacggg gaaattgccg cggtcgatgc ggtgattgga caattggttc
     3661 aagccgtgtg ggaacgtcgt ccggatacgg tggtggtgat cacggccgat catggtgagg
     3721 cgtttggtga acacggtgcg tattatcatg ggacgacggt atacgaggag caggttcggg
     3781 ttcctctgat cgtgttggcc aaaggactgc aagcgcgtcg ggtgagtgtt ccggttcaac
     3841 tcattgacgt gatgccgacg attctgcacg cgatgggtat tccggttcgt ccgcgagtgc
     3901 gaggcatcga ccttggtggc tggatgagcg gcaatgggca gggcgatggg tttgcgtttt
     3961 cggaaacgca tgatcaagtg ttgcttgcgg agggaaagtg gcgtttggtt tgtgaacgca
     4021 gggtcgacgc ttgttcgtta ttcgatcttt cggtggattc gcagcaactc cacgattgtt
     4081 ccgcgcaaga atccgaggtg ttcattcggt tgcggcagcg tttgagggct ttcgaatcat
     4141 cgcacggcgc gtacgagcga acggcctcgc gtgcggaggg caaggattta ccggaggcgc
     4201 tggtacgtgg gatggcgggt gatgcggagg ccgcggagga agtcgcgggc ttattggagg
     4261 atgcggatgt ggtgcttcgt cgcaaggcgg cggaagtctt atttcaactg gcgcgcaagg
     4321 aaagcgctcc gtcgttgagc ttggccatat cgcgggatga agatggggaa gtacggcaat
     4381 ggtgtgcgtt ggcgctcacg cgtcttgggc acggggctcc gttgacgctc gagttgttgc
     4441 gtggggggga tgaaaagtgg cgtcgtttgg cggcgttggt tcttgcggag tcgggagaac
     4501 cgcaaggggc aggcgtgctg ttggcgtggt gggaaacgca agacccctcg atcgagcagc
     4561 gcaaacaaat cgcccatgcg cttggcaaaa tcggctggaa agcggctgta gttccattgt
     4621 gtaagtggtt agaccatgag caattgcggc cgatattggc ggaagcgctg gcgcgtcttc
     4681 acgatcccta cgcaaaaatt gttcttttgc gctatttttc gcaagaacga tatctgcata
     4741 cccgtctcat tttggcgcgg tcgcttgtgg ccttgggggc gtctcgggag atggctcctg
     4801 cgctcatgcg ttttcttggt gttgcggatc cattggaagg gggggttggg attgcccgcg
     4861 aggcaaaaat tctcgacgcg ttgggagggc cgaatgagaa gagtcttgcg gagattagca
     4921 aagcgggatc tgaaggcgtc gatgtgtccg tgattgtgcc gaaaggggga aacggaaaag
     4981 ggtatcgcgc gatcgtgcgt gttcggtcga aacaggcggg gcgggtgcag ttgagtcgtg
     5041 gggatcgtgg aggtcgttcg tctacgatgg cgtcgaattc ggcggttgtt ttcgacgtac
     5101 cggccggatt gtcggaggtg catgcggagt tgggcgatgc ttttggggcg atgggaagcg
     5161 gaccgctgca tgttgtgctc gtgccgagcc cgggcgtgca agtggaaggg tttgtgattg
     5221 tgccgctagc ggatgaaata ccgcctcctg caccggagcc atgggttccg gacacgttgg
     5281 gggatgggtc ggtcgagtcg atgaagtagg gggagttgtc aggagttggc gttttggggg
     5341 tgtttccggc tacactgggg acgcatcgtt cgaccgtgtc gcagtgcgat aaacgcgcac
     5401 ggttcatgaa acgtgatttt ctgaccaaac gggaataaac ttcaaacgct gatgtttccg
     5461 cgtgatccgc tccctgattc gataggaccg tacaagatag tgcgtcgtct ggcatcacgg
     5521 cgtggggctg atttgtattt gggccagcat gctggtccga tggggtttca gcgggattgt
     5581 gtattgaagc tggtgccgag tccggcggcg agtggggatt cgaggctggc cgaagagctt
     5641 gcgcatgaag cgagtatttg ttcgaccttg catcatccgg cggttgtgcg gatgtatgat
     5701 tttttcgagc atggggacaa tttggtgctg gtgttcgagc actttgcggg cgtgtcgtta
     5761 gcgcgattgc tttcgcattt tcgtcgaagg cgtcagcgag tgccggacgc ggcggtatgg
     5821 tatttatcgc atcggttatt tggggcgttg gcgcatgcgc atgcgttgtt ggatgagaag
     5881 gggaaaagga ctccggtgat ccatcgcgat gtgcaaccgg cgaatttgat tgtgtcgcac
     5941 gatgcgaatg tgcgtcttgc gggttttgga attgcgaaga tagcggggac acctgcaacg
     6001 acggcggttg gatttgtgaa ggggacgccg gcgtacatgg cgcctgagca ggcggcggga
     6061 gagaaggtaa ccgagcgagt cgatgtatat gcggcggggt tggtggtgtg ggagatgctg
     6121 tctggtcgtt cggttaggcc gcctggtgac attgggcaag gggcggagtt gcttcgattg
     6181 attgctgggc gacgattcga gccgattgct tcgttacggg gtgacattcc tcgcgagatt
     6241 gcggcggcgg tggacgcgtg tttgatacca gcggcggagc gtcgcacgat tcgatgtcgc
     6301 gaggtggagc gttggttgga gaaagtggtt gatgtggagg cggggaaaaa ggagctgcgc
     6361 gagcggttgg tgcagcttcg caatgcgaac gtgaggacga cggcgccgag agtttcggcg
     6421 gcacctcggg gaagtcaacc gccgcatccg ttgccctctt cccaaccgcg cgcgtttccc
     6481 tctcgatttc ctggtttagc cactcgcgtg acggggcgtc ccagtgagcg tgaggagggg
     6541 gaggaacccc gatcgtcgcg tcgtgttcct tcttttcgag cctcatctta tcccgcgccg
     6601 ccgttgacat cggaaaaaga tacggcggat gccgaaccga agaaaaaatc gctattggga
     6661 cttgcgcccg cgcttccggc tttcacatcg ccgatgccgg acagggtcga acctgcgaga
     6721 acttcgtatg atgcggtagc tccaccaccc atggatgtgg aggcaga
//
LOCUS       DAOSOR010000312         6747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_11382_length_6747_cov_4.215481, whole genome shotgun sequence.
ACCESSION   DAOSOR010000312 DAOSOR010000000
VERSION     DAOSOR010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6747
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11382_length_6747_cov_4.215481"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(241..738)
                     /locus_tag="PK710_15720"
     CDS             complement(241..738)
                     /locus_tag="PK710_15720"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOT11229.1"
                     /translation="MNIVAPDSSEPLELMGFRIEHWRFAVRLSQVKTSMMPCPVTRVF
                     LTPPFVSGIISLHGSIVCVLDLGKLLALSSSGAKFRRFVVLSDNGVEAAIPAHDVFRI
                     PDVNPSEIEPVPANLPPDHRMLLEGVINQAAREDVHPEVGNTITLLNAKTLFDSPPLR
                     ALRGL"
     gene            complement(743..2743)
                     /locus_tag="PK710_15725"
     CDS             complement(743..2743)
                     /locus_tag="PK710_15725"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT11230.1"
                     /translation="MNNTFLSRLRSIRSRIILSITGLLLLFLATTVLMSRYQLGIALD
                     RGAFLAKHLGRFENTTAFMARAMSAVVESIGNDQDLVRCLAARTTPDVESAAPTSACD
                     PENRWKTARGVLGAALEPELMLLTDVHDVPVGKAPEGITAERLRRQLFYQRIAQGMVL
                     SGYVQLEGKPYFATGAPIRDSTGRRVGTILVGFSLDHFFMDFTKASDDKKNKWQNLFY
                     LVDGNVIGSTLGEVIDPASLAKAYEPANRHHDREGSRTFEVLMLGDTMYDFSSIPRTI
                     FVEDKEKRGELVMARIRTGFLQDRNRVEGPILWTGGFSLIVSILLGFLVARGIVRPLQ
                     GFIGYTEQMAKGGADLTHRFPVDGNDELAQLARNLNAMLDHLQMLFNGVKASALEVGN
                     SAAEISVTAGQLHQRSREESVKVEEIATAVSEMNQMIQQLSSYSQKAADHAKTGGEAV
                     GEASNAIIDIRKTVVDVSEHVKTLGERSARIGNIVETIGQIADQTSLLALNASIEAAH
                     AGEHGKGFAVVANEVSNLAERVNKSARQIEEQLAQIRLLTEQAVRRMGDATNTVDAGV
                     IKVDSTFRGLNEMMGLVQEIGEREKEQAAVSDNIARNMEDIYMLVREGLSASEQTVSE
                     GDRLKRLGEALLASVEQFKTDDAHAPQPPGPKALLPAKPGQS"
     gene            2938..3555
                     /locus_tag="PK710_15730"
     CDS             2938..3555
                     /locus_tag="PK710_15730"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT11231.1"
                     /translation="MHQEHHHCSHGRLFPAEAAARLDEPTRKSRQPPETLIGLLDLFS
                     GAKVLDFGAGTGFFAVPIAQHLRKLGANGKVWAVDSQPAMFDHLRAKLAGLDLAPIET
                     LVGSTDELLQMEAGNIDRVLLANVLHEVPDRPKLYAALAHILAPQGLVLIVDWHPEGS
                     TDHGPPKEHRLDPKSVGESLSQSGFNRIEISPCFSDHYSVRAIRS"
     gene            complement(3649..5748)
                     /locus_tag="PK710_15735"
     CDS             complement(3649..5748)
                     /locus_tag="PK710_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11232.1"
                     /translation="MPVSDATQASDTGEARNPTTIPLAVLPTVVVDDHFPVAAPMVST
                     GTLRKRNSVCMGLLASCPALRYRLATMRAIISRTMGLCLIAVGFVACGESEQTPQNTS
                     DSGVIGDAIPDSFTDSADPETASPPVDTGAEAAQDVSSDVPIEAAMDVVQDVPSEASV
                     GPCGRPWNHDVDAPYAIEATWMFGRKDACAWQDVLEKFHRAGGQWVWQFGPELKPRTR
                     QDLESDPAFSDCKIGAVSCIQHAIDSVGESRIEGFLSYSFGQAYSSAIAACPNLDQQV
                     TIGSTTWHRIVLPTTSSQGSCPSSGKVWVMFAVSDGMDSEQLLLDGADALGMKVILGM
                     PGIAHSKTKPWDVEPTLLAAGKEWSRRVFLDYQARAALHPSFAGTYQTFEVPLIATGL
                     DAAYGMYGQLAELFHGLFPTKLFALSPYWAANKLHGNSTAADVSAGVKRLAMLGIDII
                     APQDGRGTSKGALYWPYEKDKPIEQVDPELAKASSVPAGATFAQTYNASTFELFLAAR
                     KGIDEANAAGAHATLWANFEAFEPTTTEPCSYVTELGRTSLKRLDRAITMGAGPVQHV
                     ISYMYDPLFTCTTTTQPKALIDEILEQAWRPLVDTAFKFSSGGKEGIIVRGYHVADRN
                     PRFSLTWYDSAWTVQTTTVVPSWTNPTWGLDHEARPFAQEAFIPFEFKGLAPAFWIHI
                     RAENDAGSASESFSFAY"
     gene            complement(6413..>6747)
                     /locus_tag="PK710_15740"
     CDS             complement(6413..>6747)
                     /locus_tag="PK710_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11233.1"
                     /translation="PLRLRLRAAVANRGALGVSDGVDVAFFKREKSDWSCLGVVQTAV
                     DLLPGDVTEVTLDYTLNNEIDVELEFKAVVDSNCDGVGKHNECEVGGEDNNDDTATGM
                     CSSAMPPS"
BASE COUNT         1372 a         1723 c         2135 g         1517 t
ORIGIN      
        1 gcccaggtca ttgcgttgct ggcgctgggc tatcctgatt tcgttccgcc gccgcctgct
       61 cgggagagct acaagacgca cattcgttcg gtggagtaag cttgcggaac accgagagcc
      121 gcgcgatcga gcccttccct ctattagcgt aactatcgga atttattata ttttatccga
      181 agccgacaag gaaaatcgcc cccttcttcc gcgcagggta gcgacaattc tctcgctggc
      241 tcatagtccc cgcaaagctc gaagcggtgg ggaatcgaag agagttttgg cgttgagcag
      301 cgtgatggta ttgccaacct cggggtgaac gtcttcgcgg gcggcctggt tgatgacgcc
      361 ttcgagcagc attcgatggt cggggggcaa gttcgcggga acaggttcga tttcggaggg
      421 gttcacgtct gggattcgaa atacatcgtg ggcggggatg gcggcttcca cgccattgtc
      481 ggagagcacg acgaatctgc ggaatttggc tccggaggag gagagggcga gcaattttcc
      541 gaggtccagc acgcaaacga tggagccgtg gaggctgatg atgccgctga cgaagggggg
      601 ggtgagaaaa acgcgggtga cggggcaagg catcatcgag gttttgacct gggacaggcg
      661 cacggcgaag cgccaatgtt cgatgcgaaa gcccatgagt tcgaggggtt cgctggagtc
      721 cggggcgacg atattcatcg ttttagctct gtccgggttt ggcggggagc agggctttgg
      781 gtcccggggg ttggggagca tgggcgtcat cggttttgaa ctgttcgacg cttgcaagca
      841 gggcttctcc caagcgcttg agtcggtcgc cctcggagac ggtttgctcg ctggcgctga
      901 gtccttcgcg cacgagcatg tagatatctt ccatattgcg ggcgatattg tcggagacgg
      961 cggcctgttc tttttcgcgt tcgccgattt cctgaaccaa tcccatcatt tcgttcaggc
     1021 cgcggaaggt ggaatcgact ttgatcaccc cggcgtcgac ggtgttggtg gcgtcgccca
     1081 ttcgtcgcac cgcctgttcg gtgagcaagc ggatctgcgc gagttgctct tcgatttgtc
     1141 ttgcggattt attgactcgt tcggccaggt tggacacttc gttggcgacg accgcgaatc
     1201 ctttgccgtg ttcgccggca tgggcggctt cgatggaggc gttgagggcg agcaagctgg
     1261 tttgatcggc gatttgtcca atggtttcaa cgatattgcc gatgcgcgcg gagcgttctc
     1321 ccagggtttt gacgtgttcg gaaacgtcga cgacggtttt tcggatatcg atgatggcgt
     1381 tggaagcttc gccgacggcc tcgccgccgg tttttgcgtg gtcggcggct ttttgtgaat
     1441 aggaagagag ctgttgaatc atctggttca tttcgctgac ggcggtggcg atttcttcga
     1501 ctttgacgct ttcttcgcgg cttcgttggt gcagttggcc ggcggtcacg gagatctcgg
     1561 cggctgaatt tccgacttcc aaggcagagg ctttgactcc gttgaacagc atctgcaggt
     1621 gatccagcat ggcattgaga ttgcgggcca attgggcgag ttcgtcgttg ccatcgacgg
     1681 gaaatcggtg ggtgaggtcg gcgccccctt tggccatctg ttcagtgtag ccgatgaagc
     1741 cttgcagagg gcgaacgatg ccgcgggcga ccagaaagcc gaggagaatg gagacgatga
     1801 gcgagaagcc gccggtccac aggatggggc cttcgacgcg gtttcggtcc tggagaaagc
     1861 ctgtacgaat gcgtgccatc accaattcgc cgcgtttttc tttgtcttcg acgaagatgg
     1921 ttcgggggat ggaactgaag tcgtacatgg tatcaccgag catcaagacc tcgaaggtgc
     1981 ggcttccttc tcggtcatgg tgtcgattgg ctggttcgta agctttggcg agggaagcgg
     2041 ggtcgatgac ttcccccaag gtcgaaccga tcacatttcc atcaactaag tagaaaaggt
     2101 tctgccattt gttttttttg tcatcggatg ctttcgtgaa gtccatgaag aagtggtcga
     2161 gggagaagcc aaccaggatg gttcccacgc ggcgtccggt gctatcgcga atgggcgctc
     2221 cggtagcgaa ataaggtttt ccctcgagtt gtacataacc ggataggacc atgccctggg
     2281 caatgcgttg atagaaaagc tgtctccgaa ggcgttcggc ggtgattccc tcgggagctt
     2341 ttccgacggg aacatcgtgg acatcggtga ggagcatcaa ttcgggttcg agggcggcgc
     2401 ccaacacgcc acgggcggtt ttccagcgat tttccgggtc acaggcggat gtgggggcag
     2461 cggattcgac gtcaggggtg gttcgtgcgg ccaggcatcg aaccaaatcc tgatcattgc
     2521 cgatcgactc caccacagcg gacatggctc gtgccatgaa cgccgtggtg ttttcgaatc
     2581 ggccgaggtg tttggcgaga aaggcgccac ggtccagagc gatgccgagt tgatagcgtg
     2641 acatcagcac ggtagtggcg agaaatagca gcagcaatcc cgtaatggac aggatgatcc
     2701 ggcttcgaat cgacctgagg cgtgacaaaa acgtgttgtt catgctcgaa catccatcct
     2761 cgtagccgca cggcggcact cgattctttc gtcaagcttg gttccaagcc caaacaaggt
     2821 agcacagagc tgtccgaacc gcgtaattcc caatggaccg gggtttggaa tggatggtga
     2881 cggtcctgtt cagcggtagc cctggacaat cacgggcggt ggcccgaggg ttgggtcatg
     2941 catcaagagc atcatcattg ttcacacggg cgcctatttc cggcggaagc agcggcaaga
     3001 ctcgatgaac cgacgcgaaa gagccgacaa cctcccgaga cgctcatcgg tcttctcgac
     3061 ctattttccg gggccaaggt gcttgatttt ggggccggga ctggtttttt cgcggttccg
     3121 atcgcgcagc atctgcgaaa actaggcgct aatggcaagg tatgggcagt cgacagccaa
     3181 ccggccatgt tcgatcatct tcgggcgaag cttgcagggc tcgacctggc cccgatcgaa
     3241 acgctcgtgg ggtccaccga tgaattgctt cagatggagg caggaaacat cgaccgggtg
     3301 ttgcttgcga atgtcctgca tgaagtcccg gatcgcccga agctgtacgc ggcccttgcc
     3361 catatcctcg caccgcaagg attggtgttg atcgtcgact ggcaccccga agggagcacg
     3421 gatcatggtc cccccaaaga gcatcgtctg gatccgaaat ccgtgggtga atcgctatcc
     3481 caatcgggat tcaatcgtat cgagatatcc ccttgtttct cggatcatta ttccgttcga
     3541 gccattcgat cgtagagatc ccatacggat aggatgagga gaggattttc gtcggcgaag
     3601 cggccatgct ttgttcgaag aaggtgagcc caccggcgaa aggcggattc aatacgcgaa
     3661 gctaaaggat tcggaggcgg agcctgcatc gttttcggcg cgaatatgga tccaaaacgc
     3721 tggcgcgaga cccttgaatt cgaagggaat gaatgcttcc tgagcaaacg ggcgcgcctc
     3781 gtgatccagc ccccaggtag gattggtcca tgaaggcacc acggtagtcg tttgcaccgt
     3841 ccaagccgaa tcataccaag tcaaagagaa gcgaggatta cggtcggcca cgtgatagcc
     3901 gcgcacgatg attccttcct ttccgccgct cgaaaacttg aaggcggtat ccacgagggg
     3961 gcgccatgcc tgttcgagga tctcatcgat cagcgctttg ggttgggttg tggtcgtaca
     4021 ggtgaacagc ggatcataca tatacgagat gacgtgttgc acagggcccg cccccatggt
     4081 gatggcgcga tccaagcgtt tgagggaagt tcttcccaat tcggtgacgt acgagcatgg
     4141 ttcggtggtg gtgggttcga aagcttcgaa gttcgcccat aacgtggcgt gagcccccgc
     4201 ggcgttggct tcatcgatac cttttcgtgc ggcaagaaaa agctcaaaag tcgaggcgtt
     4261 ataggtttgc gcgaaggttg cccccgcggg cacggaagag gctttggcaa gttcgggatc
     4321 gacttgctcg atgggtttgt ctttttcgta aggccaatac aatgccccct tcgaagtgcc
     4381 acggccatct tgaggcgcaa tgatgtcgat gccgagcatg gcgagacgtt tcacacccgc
     4441 cgaaacatcc gcggcggtgg aattgccgtg gagtttgttc gctgcccaat agggcgaaag
     4501 agcgaagagc ttggtgggaa acaagccatg aaatagctcg gccaactgtc catacatgcc
     4561 gtaagcggca tcgagaccgg tagcgatgag aggcacctcg aaagtctggt acgtgcccgc
     4621 aaaacttggg tgcaaggccg cacgggcctg gtaatcgaga aagacccgtc gagaccattc
     4681 tttgccagcc gccagcaagg taggttccac atcccagggc ttcgttttcg aatgggcaat
     4741 gcccggcata ccgaggataa ccttcattcc aagagcatcc gcgccatcca acaggagttg
     4801 ttccgagtcc atgccatcgc tcaccgcaaa catgacccat acctttccgg acgatgggca
     4861 cgagccttgc gacgaggtgg tgggcaatac gatccgatgc catgtcgtgg aaccgatggt
     4921 gacttgttgg tcgaggttgg gacaggcagc gatggcgctg gaataggcct gaccaaacga
     4981 gtagctgaga aaaccctcga tcctgctttc tccgacgcta tcgatggcgt gttggatgca
     5041 gctcacagca cctattttgc agtccgaaaa cgcgggatcg gattcgagat cctggcgtgt
     5101 gcgaggtttg agttccggac cgaattgcca gacccactgt cctcccgcac gatgaaattt
     5161 ttccaacaca tcttgccaag cacaagcatc cttgcgtcca aacatccatg tggcttcgat
     5221 cgcataggga gcatcgacgt catgattcca gggtcgtccg catggcccca cggacgcttc
     5281 ggatggcacg tcctgcacca catccatggc ggcttcgatg ggaacgtcgg agctgacgtc
     5341 ttgcgctgct tctgctccgg tatcgacggg gggtgacgcg gtttcggggt ccgcgctgtc
     5401 ggtgaacgag tcgggtatcg cgtcgccaat aactccggaa tcgcttgtat tttgtggagt
     5461 ttgttcggat tcaccacagg ctacgaagcc gaccgcgatc agacagagcc ccatcgttcg
     5521 ggatataatc gctcgcatcg tggccagacg gtagcgcaag gcagggcatg aggcgagcaa
     5581 gcccatacag accgagtttc gtttgcgtag ggtccccgtc gacaccatcg gcgcagcgac
     5641 ggggaagtga tcatccacga caaccgttgg gagaaccgca agggggatgg tcgtgggatt
     5701 gcgtgcttcg cccgtgtcgg atgcttgggt ggcatccgac acgggcacgg aggggcaatc
     5761 gttacaacct attgaaatta cgtaatttta ttggtttaag ggactccgtt tccacggctg
     5821 ccgtggaccg tcgatatcct tttggtgaag ggggctcgtg ggattggtgt ggcaggaagc
     5881 gcagacgggg aaagaccgac aggcgagtca tttcagggga gcgggctgac agattcccaa
     5941 attttttcct gaattggatg ggaatacgag cagggggttg cctggacgtt gagctggtct
     6001 ttgcgtccat ttggatcacc cttgtaaaac catcaataaa tacgtctggt tgagcagtat
     6061 ggggtgatgc tcgcgggcta ccaagccttc tccaaaagct cggtgaagca ttcatcctgt
     6121 gcgaggtggg tgatggtcag ctcagccagg cttgtgctgc tgctcggctg ggatagtcgt
     6181 ccgcggatcg ccggatgcgc cctgcgtggc aaggaagcat cgagccggga tgccaacgga
     6241 ttgtctgcat atttcgatac gtggagcgta gtgttccggc ttgtggaccg atgttcgtgc
     6301 atcgtgaccg tttttgtgtg ccgtgcgccg tggccgtgac cttggggtcc aagacgacat
     6361 attgcttaca cttttgggta gcagagagtg agagaatggg cgtcagggca agtcaggagg
     6421 gaggcatggc agagctacac attccggttg cggtatcgtc attgttgtct tcgccgccga
     6481 cttcgcattc gttgtgtttg ccaacgccat cgcaattgga atcgacgacg gctttgaatt
     6541 cgagttcgac atcgatttcg ttgttcaagg tgtaatcgag ggtgacttcg gtgacgtcgc
     6601 ctggcaacaa gtcgacggcg gtttggacga ctccgaggca ggaccagtct gatttttcgc
     6661 gtttgaagaa agcgacatcg acgccatcgg agacgccgag ggctccgcga ttggcgacag
     6721 cggctcgcaa ccgtaaacgc aaggggc
//
LOCUS       DAOSOR010000313         6734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_11406_length_6734_cov_3.852373, whole genome shotgun sequence.
ACCESSION   DAOSOR010000313 DAOSOR010000000
VERSION     DAOSOR010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6734
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11406_length_6734_cov_3.852373"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="PK710_15745"
     CDS             complement(<1..131)
                     /locus_tag="PK710_15745"
                     /inference="COORDINATES: protein motif:HMM:NF019494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HOT11234.1"
                     /translation="MDNTGDKQEPRRVIKRYSNRKLYDTRDSRYVTLLQIAEMVRHG"
     gene            217..1023
                     /locus_tag="PK710_15750"
     CDS             217..1023
                     /locus_tag="PK710_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT11235.1"
                     /translation="MTHQQLPALEARRVCCAYPSSAANAIEDLSVSLDSGEWVAVIGP
                     NGAGKTTLVRVLTGLMGTSRGEVRLFGQRIDRLDRRQIARRIAVVPQHVRVAFGFTVR
                     EVVAMGRAPHQGTMLLGSATDRKIVEEVLGKTHLTAMAERRVSGLSGGEQKRVAVARA
                     LAQQSDVLVLDEAGAHLDIRHRIELLSLVRHEVDERGLACLSIMHDLNEVAQVADRVV
                     MLREGKVFEQGPVDEVMTVDNLRQIFDVDVMTGVDERRKARYFVPIRLEP"
     gene            1093..3021
                     /gene="nuoH"
                     /locus_tag="PK710_15755"
     CDS             1093..3021
                     /gene="nuoH"
                     /locus_tag="PK710_15755"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOT11236.1"
                     /translation="MRPKSFAAWITLLFLTTLAVWGCSRGHDLDLLTVLDITPRELDL
                     GDRIEIIGVDFPEGKPAEIYFEGDLYRPGRPVEKSVSIRVDAVSSSSSRIDFQLTQGL
                     HDRIAGAGADAIHTTFRGDVRVVFPSVASDGMKKSVSGAVHGAVIDVRPPTMRRAVIE
                     SRQRKGEQVLAALGIEIEDSPTSGGLKIKAVSEAPENLAAKQAGLLAGDVIKSFDGIS
                     VADKGDVIPSGTSRFADVGIERGGQLEFRKIEVSKIAKGVPTDLVGAAILLLVAVMTV
                     GVFMSPTAGIITWVERRVAGRMQSRIGPNRSGPQGFLQWLADGVKSLLKEDIVPAEAD
                     GPMFRLAPYLVFVGVSATFVVMPFGHYLIAADLDIGILFVVAVTSLVTIGLMTGGWAS
                     NNKWSLLGGIRSAAQTISYEIPGAIAIVCVVMMTGSLRMQDIIRAQGGLPWDWYMFRN
                     PVMFLLFFLYFITALAEGNRAPFDLPEAESELVAGYSTEYSGMRYVFFFFGEWANVFV
                     MSGIASALFLGGWQVPFVDAMTQGNSFWWQALGAFIFLSKAWFLVFVVIWIRWTLPRI
                     RIDQLMNLCWKWLVPGAFVAFVFTALWTLWNPEGAVRWAISGATFLAFVAIVIQFGRR
                     VAFAMRNMHANVHLNPFL"
     gene            complement(3178..5235)
                     /locus_tag="PK710_15760"
     CDS             complement(3178..5235)
                     /locus_tag="PK710_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11237.1"
                     /translation="MNHSWALRVLLGACLMLTLATCLLPGCCKFLGLRKTELLSKELM
                     PDDLHTQEVAYHNSIRLRLPFGSLKKKSRVTISSVSNAPRPDNATSDPTVYDFSVEGH
                     TQFEEPVEIVLPFTPASTTKVGLGVDALTYDESAREWKPMPFAAHKEGNSVSIYTTHL
                     SAFAMSARAGVALSPTMKIGSTPFPGSNEIMDAGTVEQIIRGDSAKAAQQGWAKSMEF
                     FGISSNTGTFIEQAVLEIPGLKSLNGVATKLGVAFGFLQLAIDLASGNTQAASINAAK
                     NLGYSSVALWGSQALQIGSVAVFIFDYTLTKVGETAWEGRTDLYQRAYALYYKEHPRK
                     GSDWSKIVLGVIDSAKSADDVHKGIDSALQSYVTAFWENELVVAEYLERVKKNAQTGG
                     GGLNEKMKAEISAAYRAEIMHLLQETVFPRIPIIIANRYRQNVSQLLGELRAGLDAST
                     TIEVIVKSKDRERSVKNIDVQILVNQEPNRWHGVTDKDGRWSMPCTTLGYLSYGAPKK
                     VKVTVPAENGKDAQSVEADLAYTPGKTTVVVELDGSGVFVGTIEDAQVVPGGDYPVKI
                     SGPVTITVASDGKATMDFSLTAKESVGKGTGVTDISSSGKLTGSLVGDDFKASGTVTS
                     TYVSKFKLPRGVNLPPGTGSGTHSAAITATGKRVGNTIRGTLQGAQGKAVRFQATQSN
                     QSP"
     gene            complement(5402..>6734)
                     /gene="asnS"
                     /locus_tag="PK710_15765"
     CDS             complement(5402..>6734)
                     /gene="asnS"
                     /locus_tag="PK710_15765"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004244664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOT11238.1"
                     /translation="GWIRTRRDSKAGLSFLSVHDGSCFEALQVVASSSLSNYATEIQR
                     LSAGCSVIVHGTIVASPGSGQAVELRANEVKVAGWVEDPETYPMQPKRHSLEYLREVA
                     HLRPRTNVIGAVTRVRHCLAAAVHRFFHEQGFYWIHTPIITTSDAEGAGQMFRVSTLD
                     FANLPRTDSGEVDFQEDFFGRQAFLTVSGQLNVESYCLALSKVYTFGPTFRAEDSNTS
                     RHLAEFWMIEPEIAFANLQDDADLAESFLKYLFRAVLQERPDDMKFFAERIDASCIDR
                     LEKFVDAAFERMDYTDAITQLQKSHRKFDFPVSWGVDLQSEHERYLSEELVGRPVVVM
                     NYPKAIKAFYMRLNDDDRTVAAMDVLAPGIGEIIGGSQREERLDVLDARLDELGLDKQ
                     AYGWYRDLRRYGSVPHAGFGLGFERTIQYVTGMANIRDVIPFPRYPGHAEY"
BASE COUNT         1357 a         1800 c         2014 g         1563 t
ORIGIN      
        1 tcgccgtgcc gcaccatctc cgcgatttgc agcaaagtga cataccgact gtctcgggtg
       61 tcgtacagct tacggttgga gtagcgcttg atgacgcggc gcggttcctg cttgtcgccc
      121 gtgttgtcca cgttagctcc ttcctaagct atgattccgt gtgaatgcat cgtttccgca
      181 attttcgcaa agcgtcaaga gggctagatg tgtaacatga cgcatcaaca gttgccagcc
      241 ctcgaagctc gtcgggtgtg ctgtgcctat ccaagctccg cggccaacgc gatcgaagat
      301 ctgtcggttt ctctcgattc gggagaatgg gtcgcggtca tcggcccgaa cggcgcagga
      361 aaaacgacat tagttcgcgt acttacggga ttgatgggga cgagcagggg agaggttcgg
      421 ctcttcggac agcgtatcga ccggttggat agacggcaga tagcgcgacg gattgccgtg
      481 gttccccagc atgttcgggt ggcgtttgga tttaccgtgc gagaggtcgt ggcgatgggg
      541 cgtgcccctc atcaaggcac catgctgttg ggctcggcaa cggaccgaaa aatcgtggag
      601 gaggtattgg gaaagacgca tcttaccgcg atggccgagc ggcgagtgtc gggattgtca
      661 ggcggtgagc agaagcgtgt ggccgtggca cgcgcgttgg cgcaacaatc cgacgttctt
      721 gtgctggacg aggcaggggc tcatttggat attcgccacc gaatcgagct tttgtcactt
      781 gttcgtcacg aagtcgatga acgtggtctc gcgtgccttt ccatcatgca tgacctgaac
      841 gaggtggcgc aggtggcaga ccgggttgtc atgttgcggg aaggaaaggt attcgagcaa
      901 ggtccggtgg atgaggtgat gacggtggat aatttgcggc agatatttga tgtggacgtc
      961 atgactggcg tggatgagcg acgaaaggcg cgatatttcg ttccgattcg ccttgaaccg
     1021 taagctgccg tgacaaaaac ctcccgcaat ctcgaccgga accatcaatc aggggtataa
     1081 gcggaaaccg ccatgcgacc gaaatcattt gcggcgtgga tcacgctgtt gtttctcacc
     1141 acactcgccg tgtggggatg ctcccgcggt catgatctcg acttgttgac cgttctcgac
     1201 atcacgccac gtgagctgga cctgggtgat cggatcgaga tcatcggagt ggattttccg
     1261 gaaggaaagc ctgcggaaat ctatttcgaa ggggatttat atcgaccggg aaggcccgtg
     1321 gaaaaatcgg tgtcgattcg tgtcgatgcc gtcagctcct cgtcgagtcg catcgatttt
     1381 cagcttacgc aaggcttgca tgatcgaatc gccggtgcag gggcagatgc cattcacacg
     1441 accttccgcg gggatgtgcg cgtggtcttt cctagcgtgg cttccgatgg catgaaaaaa
     1501 agtgtttccg gggccgttca cggagcggtc atcgacgtgc gaccgcccac catgcgtcga
     1561 gccgtgatcg agtccaggca acgcaagggc gagcaagttc tcgcggcgct cggtatcgag
     1621 atcgaagact cccccacgtc gggtggcctc aaaatcaaag ccgtgagcga ggcaccggag
     1681 aacctggcgg cgaaacaggc tgggctgctc gctggcgacg ttatcaaatc gttcgatggt
     1741 atcagcgtgg cggacaaagg agatgtcatt ccatcgggga cctcccgatt tgcagacgtg
     1801 ggcatcgagc gggggggaca gctcgaattt cgaaagatcg aagtatcgaa gatcgcgaaa
     1861 ggtgtgccca cggacctggt tggagccgcg attttgctcc tcgtggcggt gatgacggtc
     1921 ggtgttttca tgtcgcccac cgcaggaatc atcacgtggg tggagcgtcg cgtggccggg
     1981 cgaatgcaat cacgcatcgg ccccaaccgc tcaggacctc aaggattttt gcaatggcta
     2041 gccgatggtg tgaagtcgct gctcaaagaa gatatcgtgc ccgcggaggc tgatggcccc
     2101 atgtttcgtt tggcgccgta cctcgtgttc gtcggtgtga gcgcaacgtt cgtggtgatg
     2161 ccgtttggac actacttgat cgccgccgat ctggatatcg gtattctttt tgtcgtggca
     2221 gtgacgtcgt tggtaacgat tgggttgatg accggtgggt gggcatcgaa taacaagtgg
     2281 tcgctgctgg ggggaattcg ctccgctgcg caaaccatca gctatgaaat ccccggtgct
     2341 atcgcaatcg tttgtgtggt gatgatgacg gggagtctgc gcatgcaaga catcatccgc
     2401 gcgcaggggg ggcttccctg ggattggtat atgttccgta accccgtgat gttcttgttg
     2461 tttttcctgt acttcatcac cgccctggcg gagggaaacc gcgcgccttt cgaccttccc
     2521 gaggcagaga gcgaattggt ggcggggtac tcgaccgaat actccggcat gcgatacgta
     2581 ttctttttct tcggggagtg ggccaacgtc ttcgtcatga gcggtatcgc ttccgcgcta
     2641 tttctcgggg gatggcaagt gcctttcgtg gatgccatga cccaaggcaa ttcgttttgg
     2701 tggcaagccc tcggtgcgtt catcttcttg agcaaggcct ggttcctcgt tttcgtcgtc
     2761 atctggatac gctggacctt gccgcgtatt cgtatcgatc aactcatgaa tttgtgctgg
     2821 aaatggctcg tgccaggagc tttcgtggct ttcgtgttca ccgccttatg gaccttgtgg
     2881 aaccccgaag gagcggtgcg gtgggcaatc tctggcgcaa cgtttctagc ctttgtcgcc
     2941 atcgtcatcc aattcggccg tcgtgtggcg ttcgccatgc gcaacatgca cgccaacgtt
     3001 catctcaatc catttctgtg atgaggattc atcgagccca ttgaactcgc actcgctgcg
     3061 ccttttttgc ttgatgggaa gccggtgcag atcgcagtgg tgcgggtggc gtcttgcgat
     3121 cggtgggttg acaaagacga gcgaatgcgt ttgccaatga tccttgccgc gtggaactca
     3181 ggggctttgg ttgctctggg tagcttggaa acggacggct ttcccttgcg cgccttgcag
     3241 ggtgccccga atggtgttgc cgactcgctt tcccgtggcg gtaatggctg cggaatgcgt
     3301 tcccgagccc gtgcccgggg gcaggtttac gcctcgggga agcttgaatt ttgatacata
     3361 cgtgctggtt accgttccgg aagccttgaa gtcgtcgccg acgagcgagc cggtgagttt
     3421 tcccgaggag ctgatatcgg tgactcccgt gcctttgcca acggattctt tggccgtcaa
     3481 agagaagtcc atggttgctt tgccatcgga cgccaccgta attgtcacgg gacctgagat
     3541 ttttactgga tagtccccac cgggcacgac ttgcgcatct tcgatcgtgc cgacgaaaac
     3601 tcccgaccca tccaactcca cgacgactgt tgtcttgcct ggcgtgtagg cgaggtctgc
     3661 ctcgaccgac tgggcatcct taccgttttc ggccggcact gtcactttca ccttcttggg
     3721 cgcaccgtag gaaaggtagc cgagggtggt gcatggcatc gaccagcggc cgtccttatc
     3781 cgtcacgccg tgccaccggt tcggctcctg gttgacgagg atctgcacat cgatgttctt
     3841 cacggatcgc tcgcgatcct tgctttttac gatcacctcg atcgttgtcg aggcatcgag
     3901 gccagcccgc aactctccga gaagctggga gacattctgg cggtagcggt tggcgatgat
     3961 gatcgggatt cgcgggaaaa cagtttcttg aagtagatgc atgatctcgg cgcggtaggc
     4021 cgcggagatt tcggctttca tcttttcgtt gagtcctccg cctcctgttt gcgcgttctt
     4081 cttgacgcgt tcgagatact cggcaacgac caattcgttt tcccaaaagg ccgtcacata
     4141 agattgcaac gcgctatcga ttcccttgtg aacgtcgtcg gccgacttgg cgctgtcgat
     4201 gactcccaga acgatcttcg accagtccga acccttgcga gggtgctctt tgtaatagag
     4261 cgcataggcc ctttggtaca gatccgttcg tccctcccaa gccgtctcac ccaccttggt
     4321 gagggtataa tcgaagatga acaccgcgac gcttccgatt tggagcgcct gggatcccca
     4381 gagcgcaacg ctggaataac caaggttttt cgcggcattg atgctggcgg cttgggtgtt
     4441 gccgctggca agatcaatgg cgagttggag gaatccgaaa gccacaccga gcttggtcgc
     4501 gactccgttg agggatttga gtcccgggat ctccaacacc gcctgttcga tgaacgtccc
     4561 cgtgtttgat gagataccaa agaattccat gctttttgcc catccctgct gcgcagcctt
     4621 ggcggagtcg ccgcggatga tctgctcaac agtcccagca tccatgatct cgttgctccc
     4681 cgggaaaggg gtggacccaa tcttcatggt aggtgacaac gcaaccccag cacgtgcgga
     4741 catggcaaag gcactcaaat gagtcgtgta gatggacacg gagttgccct ctttgtgggc
     4801 ggcaaaaggc atgggcttcc attcacgggc tgattcgtcg taggtgaggg catcgacacc
     4861 caagccgact tttgtcgtgg aagcaggtgt gaaggggagc acaatttcaa cgggctcttc
     4921 gaattgcgtg tgcccctcca ccgagaagtc gtacacggtg gggtccgatg tcgcgttgtc
     4981 ggggcgaggc gcgttggata cggaagagat ggtgacgcga gatttctttt tgaggctacc
     5041 gaaaggaagc cgcaaacgaa tcgagttgtg atacgccact tcctgcgtgt gcaggtcgtc
     5101 gggcatgagt tccttggaca gaagctcggt ctttcgtagg ccaaggaatt tgcagcaacc
     5161 aggcaagagg caggttgcta acgtcaacat caagcaggca cccagcaata cgcgcagagc
     5221 ccaggagtga ttcattgtag gaaaaaaaat atatcactgc ctgtttcggc gtgccacgac
     5281 acacgtagag atgaaagcag ttattgcaag ggagccatcg cctccgcgaa ccatccctga
     5341 cgcataatgc ttggctcgaa cgccagcctt cgcaccacgc cgatcgctgc ctttggggcg
     5401 gtcaatactc cgcgtggccg gggtatcggg gaaacggtat gacgtcgcga atatttgcca
     5461 ttcccgtgac atattggatc gtgcgctcga atcccaaacc aaaaccggcg tgcggcacac
     5521 ttccgtagcg acgcaagtcc cgataccaac cgtaggcttg tttgtccaaa ccgagttcgt
     5581 cgagccgggc gtcgagaaca tcgagacgtt cctctcgttg tgagcctccg ataatctcgc
     5641 cgatgccggg cgccagcaca tccatggcag caacggttcg atcgtcgtca ttgaggcgca
     5701 tataaaatgc tttgatagct ttggggtagt tcatcacgac cacggggcgt ccgacgagtt
     5761 cttcgctgag ataccgctcg tgttccgatt gcagatccac tccccacgaa acgggaaagt
     5821 cgaattttcg atgggacttt tgaagctgtg tgatcgcgtc ggtgtagtcc atgcgttcga
     5881 aagctgcatc gacgaatttt tccagccgat cgatgcacga cgcatcgatt cgttcggcaa
     5941 aaaacttcat gtcgtccgga cgctcttgca acacggcacg gaacagatat ttgagaaagg
     6001 attcggctag atcagcatcg tcttgcagat tggcgaaagc gatctcgggt tcgatcatcc
     6061 agaactcggc caggtgacgg ctcgtattcg aatcctcggc gcggaacgtt gggccaaacg
     6121 tatagacttt gctcaaggca aggcagtaac tctctacatt gagctgtcct gatacggtga
     6181 gaaaggcctg gcgtccaaaa aaatcctctt gaaaatccac ttctccacta tccgtccggg
     6241 gcaagttggc aaagtcgagg gtggacacac gaaacatctg cccagcccct tcggcgtcgc
     6301 tggtggtgat gatcggtgta tggatccaat agaatccttg ttcgtgaaag aaacgatgaa
     6361 cggctgccgc caggcaatga cgcacgcgag tgacggcgcc aatgacgttg gtgcgtggac
     6421 gcaaatgcgc cacttctcgc aaatactcga gagaatggcg ttttggctgc atgggatagg
     6481 tttccggatc ctcgacccac cccgccactt ttacctcgtt cgcgcgaagc tccacggcct
     6541 gtccactgcc tggggatgcc acgatcgttc cgtgtacgat cacagagcac cccgcggata
     6601 atcgctgaat ctccgtcgcg tagttcgata ggctactgga agccaccacc tgcaacgcct
     6661 cgaaacacga gccgtcgtgg acggaaagaa atgacaaccc cgctttggag tcacgacgcg
     6721 tccgaatcca tcct
//
LOCUS       DAOSOR010000314         6713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_11450_length_6713_cov_3.433313, whole genome shotgun sequence.
ACCESSION   DAOSOR010000314 DAOSOR010000000
VERSION     DAOSOR010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11450_length_6713_cov_3.433313"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="PK710_15770"
     CDS             <1..1089
                     /locus_tag="PK710_15770"
                     /inference="COORDINATES: protein motif:HMM:NF024930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11239.1"
                     /translation="GSFSTTQNAASCTPWTTCNAGQYVSAAGSATTDRVCAACATGSY
                     STTTNAGGCSEWSICGDDEYVSTPPSSTNDRQCTPCPAGMTSEGDNAPACLLPFESIA
                     VGAEHGCGIRLLDRVTQCWGDNTYGQASPPSGVEFSQITAGYYHTCGIRRTDGKAQCW
                     GLDDDEQSTPPDEVFESLSAGHRHTCGILAGSHEAECWGTMDRTEFPVEVEFSMLASG
                     AGHTCGMRLGEPEVICWGDDSAGQATGFLRELQWISAFENYTCGVEMDDTIFCWGNMP
                     SGAPTAATFSSVSAGGTHACGLLKSDSTAQCWGTNTNGQASPPAVVSFEEIQLGLAHS
                     CGIRETDGEIQCWGANNKGQSSPPHHVP"
     gene            1227..2741
                     /locus_tag="PK710_15775"
     CDS             1227..2741
                     /locus_tag="PK710_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HOT11240.1"
                     /translation="MIRHISLLSLGLVGALFAMGCSGADGEDGKTGPAGKEGPAGQAG
                     PAGQDGLPGPAGEQGPEGPAGQDAVGTVSISGVTPAFAFLDRSKTVTISGFGTDWSST
                     TPPTVSFGPDVDVDTVSVASPTALIAAITIKPDAETGPRNVTVTNGAETLTYSAGFTL
                     ESPAKFDIVGTPAQGSIMIASVVNRDFETPFDTTYTGDGFFTPIVYTNIQMSALPGVL
                     ADPQNVQPYTMEWLMLVDVDATAGDKDLDILSGPAGQQVHFPAPKAFSLAEREAETIT
                     LGTPKTDKVTSVYGTQLYKIEGSKTEGQIVNIAVSSTKADSQPAFALLPESGKFNDLI
                     AYTNDSTLVTTPTSSVYYLVFWDSTGAFNYDFTFTTSATGPVTLLEDKEPNNTIAQAL
                     VAAAMPAYMANATLSSEADVDWVKYEAKAADVGKKFRMTTTAGDAYCDTVVAAYGSDG
                     TTLLGTESSDSNYHENHLSAAIPAAGTYYIRVSASHQGYFDVSDNRYNLMITLE"
     gene            complement(2794..4128)
                     /gene="thiC"
                     /locus_tag="PK710_15780"
     CDS             complement(2794..4128)
                     /gene="thiC"
                     /locus_tag="PK710_15780"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOT11241.1"
                     /translation="MNRAEAIARRKNSPNQSQMHFARKGIITEEMAHVANDESIDPAM
                     VRDEVAAGRLVIPANIHHLSLQPVGIGMALRCKVNANIGNSELTGTIETELDKLRISV
                     RYGADTVMDLSTGGDIDAIREGIIANSTVPIGTVPIYHALESVSSIRELTVDKILGMI
                     EHQAKQGVDYMTLHCGLLREHLPLVRRRLTGIVSRGGAIMAQWMLEHDAQNPLYTHWS
                     DILEICARYDVTISAGDGLRPGSQHDASDEAQFAELTVLGQLADRAWDKDVQIMIEGP
                     GHVPFDQIEMNVKRQIQACKGAPFYVLGPIVTDVAPGYDHITSAIGATAAAYAGAAMI
                     CYVTPKEHLGLPNLEDVRQGLVAHKIAAHAADIARHRPGARTRDDEMSKARYAFDWER
                     QFELALDPETARSYHLETKSEDCYISEDFCSMCGPRFCSMRLNRMLEERYGS"
     gene            4307..4804
                     /locus_tag="PK710_15785"
     CDS             4307..4804
                     /locus_tag="PK710_15785"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOT11242.1"
                     /translation="MSPPKTLVLQRTKEREKVAFWLGTWFGCGLSPRSPGTVGSLGAL
                     PLYFLVVRFGPVGIGITALVITLLGIVVSTRLARASGCNDPQFVVIDEVAGMLFTLAF
                     VPARWDGLLAALVLFRLFDILKPPPCRWIEKHLPPGPGIMLDDVFAAIWAIGMILIFN
                     KIGWL"
     gene            4898..5626
                     /locus_tag="PK710_15790"
     CDS             4898..5626
                     /locus_tag="PK710_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11243.1"
                     /translation="MNHSRIAIATVFVAGLLAVGCCKKKPGDSCSGTSPSCLDENNLL
                     VCEKGTLISTPCKGPNGCKVENNLIKCDISGNAAGDPCSSGDEGDTACGVDGKSQISC
                     HSGKYEVALCRGPDGCKTQGNTSICDLSIAEEGQECKGPGAINTYACSVDGSKVLKCT
                     NGQFNMSGYCRGKKCEVKDGKVGCPDPIALVNDPCDTSSLGSYACAVDGSALLVCRGS
                     KWIVDEKCRGKTKCKEQNNQVGCM"
     gene            complement(5643..6167)
                     /locus_tag="PK710_15795"
                     /pseudo
     CDS             complement(5643..6167)
                     /locus_tag="PK710_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
     gene            complement(6209..>6713)
                     /locus_tag="PK710_15800"
     CDS             complement(6209..>6713)
                     /locus_tag="PK710_15800"
                     /inference="COORDINATES: protein motif:HMM:NF014077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOT11244.1"
                     /translation="VSGLNAGLNLGLDTYYSGTVAGAREAAMRGVPGMAVSAEPGADG
                     KRAAHLSSRLAWHFLQIFDRAEPVVLNVNIPARGSWELRHTRLGRRVYGEGIEFRRSP
                     RGREYFWIGGAGVMKDECTPGTDTRALHEGAIGVTNLPLTPFDSRGDDIIDRMIRAWQ
                     AEQRSER"
BASE COUNT         1489 a         1963 c         1810 g         1451 t
ORIGIN      
        1 ggctctttca gcacaactca aaatgctgcc agttgcaccc catggaccac ctgcaacgcg
       61 ggccaatacg tttccgcagc aggctctgcc actaccgatc gggtgtgtgc tgcttgtgct
      121 actggctctt acagcaccac gaccaatgct ggcggttgct ccgaatggag catttgtggc
      181 gacgatgaat atgtatccac ccctccaagc tctaccaacg accgccaatg cacgccatgc
      241 cccgcaggta tgacaagcga aggggacaat gcccctgcct gtttgcttcc gttcgagtca
      301 attgcggtcg gagccgaaca cggttgcggt attcggcttc tcgaccgcgt cacccaatgc
      361 tggggagaca acacgtatgg tcaagcttct ccgccgtctg gagtcgaatt ttctcaaatt
      421 accgcgggat attatcatac gtgcggcatt cgacgcaccg acgggaaagc acagtgttgg
      481 gggcttgacg acgatgagca atcaacaccg ccagacgagg ttttcgagag ccttagtgcg
      541 ggacaccggc atacctgtgg aatcctcgct ggctcccacg aggcggaatg ctggggaacc
      601 atggatagga ccgagtttcc cgttgaggtc gagttttcga tgctggcgtc gggcgccgga
      661 cacacctgtg gaatgcggtt gggcgagcca gaagtcattt gctggggcga cgacagcgcg
      721 gggcaagcta ccggtttctt acgagagctg caatggatca gtgcgttcga gaactacacc
      781 tgcggcgtgg aaatggacga caccatcttc tgctggggca atatgcctag cggcgctccg
      841 acggcagcga ccttctcgag cgtgagtgcg ggtggcacgc atgcttgtgg actattaaaa
      901 tccgactcca cggcacagtg ttggggaacg aacactaacg gccaggcaag cccgcccgct
      961 gtagtttcct ttgaggaaat tcaacttggg ctcgcgcatt cttgtgggat ccgcgagacc
     1021 gatggcgaga ttcagtgctg gggggccaac aacaagggcc aatcctcgcc tccccaccac
     1081 gttccataaa cacctacgga aaaaatccaa tatcttggga tgactcggct tgcgcaatga
     1141 cgttttcgct gccataacgt ttgcgcaacg tgggtgctcg ttgcctccac aaggggagca
     1201 aggggcgtca ataacaggag agacagatga ttcgtcatat ttctttgttg agcttgggat
     1261 tggtcggcgc attgttcgcg atgggatgca gcggcgcgga tggtgaagac ggcaagacgg
     1321 gtccggcagg aaaagagggg ccggcgggac aggcggggcc agcgggacaa gatgggctgc
     1381 ccggaccagc cggagagcaa ggacctgaag gacctgcagg acaagatgcc gtgggcaccg
     1441 tcagcatcag cggcgttacc cccgcctttg ctttcctcga ccgctccaaa accgtcacca
     1501 tcagtggatt cggcaccgac tggtcctcca cgactccccc caccgtctcc ttcggaccag
     1561 atgtcgatgt agatacggtt tctgtggcta gccccacggc gctcatcgcc gccatcacga
     1621 tcaagccaga cgccgaaact ggcccgcgga acgtcacggt taccaacggt gcggagacat
     1681 tgacatattc ggcgggattc acgctcgaat cacccgcgaa gttcgacatt gtgggtaccc
     1741 ccgcgcaggg ctcgatcatg atcgcctccg tggtcaatcg ggacttcgaa acgcctttcg
     1801 atacaaccta cacgggagac ggcttcttca ctcccatcgt gtacacgaat attcagatgt
     1861 cagcactccc gggagttttg gctgatcccc aaaacgtgca gccttacacg atggaatggc
     1921 tgatgctggt cgacgtcgat gcgacagcag gggacaaaga cctggacatc ctcagcggtc
     1981 ccgcgggaca gcaagtccat ttccctgccc cgaaggcttt cagccttgcc gaacgcgaag
     2041 cagaaacgat caccctaggc accccgaaaa cagacaaggt cacctccgtg tatggaaccc
     2101 aactgtataa aatcgagggt tcaaagacgg aaggccagat cgtcaatatc gctgtcagtt
     2161 cgaccaaagc agactcacag ccggcttttg ctttgctgcc ggaaagcgga aaattcaatg
     2221 atctcatagc gtatacgaac gacagcacgc tcgtcaccac accgactagt tccgtgtatt
     2281 acctggtctt ctgggatagt acgggtgctt tcaactatga tttcacgttc acgacatcgg
     2341 ccaccggacc ggtcaccctt ctcgaggata aggaacccaa taacacgatc gcgcaagcct
     2401 tggtggctgc tgccatgccc gcgtacatgg ccaacgctac cttgagcagc gaagccgatg
     2461 tggattgggt caagtacgaa gcgaaagcgg ccgatgttgg gaagaaattc cggatgacga
     2521 ccacagcggg cgatgcctat tgtgacacgg tggtagcagc gtatggcagc gatggaacca
     2581 cgctattggg aaccgagagt tcggactcca actaccacga gaatcatttg agcgcggcaa
     2641 ttcccgcggc aggcacctat tacattcgcg tgagcgccag tcatcagggt tacttcgatg
     2701 tgagcgacaa ccgctacaac ctgatgatca ccttggagta gcgtaagaaa tgagggcgcg
     2761 ggtacaacaa cccgcgcctt gtctaaagct cgttcaactc ccgtatcgct cttccagcat
     2821 tcggttcagc cgcatcgagc agaaccgagg cccgcacatc gagcaaaagt cctcagaaat
     2881 ataacaatcc tcggacttgg tttcgagatg gtagctgcgc gcggtttctg gatcgagcgc
     2941 caactcgaat tgccgttccc aatcgaaagc gtatctcgct ttggacattt cgtcatcgcg
     3001 cgttctcgcc ccgggcctat gacgagcgat atccgcggca tgagccgcta ttttatgagc
     3061 caccaaccct tgtctcacat cctccaagtt cggcaaaccc aggtgctcct tcggtgtgac
     3121 ataacaaatc atcgcggccc ccgcatacgc cgccgccgtg gcaccaatcg cgctggtaat
     3181 atgatcgtag cccggtgcga cgtccgtgac gatgggtccc aaaacataga acggagcccc
     3241 tttgcaagcc tgaatctgac gtttgacatt catctcgatc tgatcgaacg gcacatgccc
     3301 cggcccttcg atcatgatct gcacgtcctt gtcccaagct cggtccgcga gctgcccaag
     3361 aacggtaagc tccgcgaatt gtgcttcatc cgaagcatca tgctggctac cgggacgcaa
     3421 tccatcgccc gcggaaatcg tcacatcata tctcgcacaa atctccagaa tatcagacca
     3481 atgcgtatac aaggggttct gcgcatcgtg ctccaacatc cattgcgcca tgatcgctcc
     3541 gcctcgggat acgatccccg tgagtcgtcg tcgcacgagg ggcaagtgct cccgcagcag
     3601 cccacaatgc agggtcatgt aatcgacgcc ttgtttggcc tggtgttcta tcatgcccaa
     3661 aatcttgtcc accgtaagct cgcgaatgga agaaacgctt tccaacgcgt ggtaaatcgg
     3721 cacggtgcca atgggaaccg tcgagttcgc gatgatgcct tcccgaatgg catcgatatc
     3781 tccacccgtc gataaatcca tcaccgtatc agccccgtaa cgcacggaga tgcggagctt
     3841 atcgagttct gtctcgatgg tgcccgtcag ctcgctattg ccgatatttg cattgacttt
     3901 gcaccgcagc gccatcccga tccccacggg ctgcaacgag agatgatgga tgttggcggg
     3961 aatgaccagg cgtccggcag caacttcgtc acgaaccatg gccggatcga tgctttcatc
     4021 gttggcgaca tgggccatct cctcagtgat gattcccttg cgggcgaagt gcatttgact
     4081 ttggttcgga ctattttttc gtcgtgcgat cgcttctgct cggttcatgg ctcctcacgg
     4141 tggtcgccca gacgaccttc caatcaaggt aggttcttgt aagcagcccg ttcgcagagg
     4201 gcaaccctga tcgccgaaag cgatggagat tcgaagtcgc gaaacgcgcc atgtccaatt
     4261 gcgctcgttt ttttgtaaca cgattgctgc tagggtcatg atttcgatgt ctccacccaa
     4321 aacgttggtg cttcagcgaa ccaaagagcg ggagaaggta gccttttggc tcggtacttg
     4381 gtttggatgt gggctgtcgc cccggagccc aggcacggtt ggttcgcttg gtgccttgcc
     4441 tctttacttt ttggtcgtgc gttttggccc tgttggcata ggcatcaccg cgcttgtcat
     4501 cacgttgctc ggaattgttg tgagcacgcg tctggcacga gcctcaggtt gcaatgatcc
     4561 acaattcgtc gtgatcgatg aggttgctgg catgcttttt acccttgcct tcgttcccgc
     4621 aaggtgggat ggcctgctcg cggctctcgt gcttttccgg ctcttcgaca tcctcaaacc
     4681 tcccccctgc cgatggatcg aaaagcatct gccccctggt ccaggcatca tgctcgatga
     4741 cgtcttcgcc gcaatatggg ccatcggtat gattttaata ttcaataaaa ttggatggtt
     4801 ataaattttc cgttgtcgaa gagcagcgag catcccatga acacgctcga cattgggcag
     4861 actctcatcc actctttcaa cctttggaaa tcatctcatg aaccattccc gaatcgccat
     4921 cgccaccgtt tttgtcgcag gcctgcttgc tgttggatgc tgcaaaaaga agccggggga
     4981 ttcttgctcg ggcacttcgc cctcctgctt ggacgaaaac aatttgttgg tatgtgagaa
     5041 gggcacgctc atcagcaccc cttgcaaggg accgaacggc tgcaaagtcg aaaacaatct
     5101 gatcaaatgc gatatttccg gcaatgcggc aggcgatccc tgtagcagtg gcgatgaagg
     5161 ggatacggct tgtggtgtcg atggcaaatc acagatctcg tgtcactccg gcaaatacga
     5221 ggtggctctt tgccgcggcc ccgatggctg caagacccaa ggcaacacat cgatttgtga
     5281 cctttccatc gcggaagagg gacaagaatg caaaggtcca ggagccatca atacctatgc
     5341 ctgctctgtc gatggcagca aagtgctgaa atgcacgaat gggcagttca atatgtcggg
     5401 ttattgtcgg ggaaagaagt gcgaggtcaa ggatggcaag gttggctgcc ccgatcccat
     5461 cgccctcgtc aacgatccct gtgatacttc ttccctcggt tcctacgcct gcgccgtcga
     5521 tggatccgct ttgctcgtat gccgggggag taagtggatc gtcgacgaaa aatgcagagg
     5581 caagaccaag tgcaaagaac aaaacaatca agtgggatgc atgtaaggag cggaattcgg
     5641 tttcactccc ctgccccgta ttcgagcaac gatacgatcg gaacgtcccc gagtttttca
     5701 cgccctttca gaaacgacag ttccaccaca aaggcataga gcttcacctt tgccccctga
     5761 ttgcccacga gttgcgctgt cgccgcggcg gtgcctcctg tcgccaacaa atcatcgacg
     5821 atagccaccc ccatcccctt gcggagagca tctttatgca tttccaaggt attggagcca
     5881 tattcgaggg tgtaggaaac tcgatcgaca gcggcaggca gcttgccggg cttgcgtacg
     5941 gggacgaaac cgattccgag acgactggcg agcgcggcac caaaaatgaa ccctcgggat
     6001 tcgatcccga ccacggcatc gatgccgcct gcccacatcg attccgcaag ggcgtcgagc
     6061 acgatttgaa aggctttggc gtcccccagc aacggtgaaa tatctttgaa cagtatgcca
     6121 ggctcaggaa agtcaggcac attgcgaatc aagccgtcca cgtactgcct gcgcccactt
     6181 ccgtgccaca aatcctgata cttatcgctc atcgttcgct cctttgctcc gcttgccacg
     6241 cacgaatcat cctgtcgatg atgtcatctc cccgagaatc gaaaggggtg agaggcagat
     6301 tcgtcacgcc aatggctcct tcatgcaaag cccgcgtatc ggttcctggc gtgcattcat
     6361 ccttcatcac acccgctcca ccgatccaaa aatactcacg ccctcgaggg cttcgtcgaa
     6421 actcgattcc ttcgccatat acacgtcttc cgagccgggt gtggcgaagc tcccacgaac
     6481 cgcgggcagg aatattgaca ttgaggacca cgggttcggc tcgatcgaag atttgcaaga
     6541 aatgccaagc aagtcgcgag cttagatgcg cggcacgctt tccatcggct cccggctccg
     6601 cggatacggc cattcccggt accccccgca tcgcggcttc cctcgctccc gccacggttc
     6661 ccgaatagta ggtatcgaga cctagattca gccccgcgtt caagcccgaa acc
//
LOCUS       DAOSOR010000315         6690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_11502_length_6690_cov_4.066616, whole genome shotgun sequence.
ACCESSION   DAOSOR010000315 DAOSOR010000000
VERSION     DAOSOR010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11502_length_6690_cov_4.066616"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..1409
                     /locus_tag="PK710_15805"
     CDS             159..1409
                     /locus_tag="PK710_15805"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT11245.1"
                     /translation="MNDDTLPFSASLATANTMVARVCTTCGKTYPPSAVYCSIDGSEL
                     SAIATDDTDPLVGQVLDGRWVIRRRIGAGGMGAVYAASQLSVNRQVAIKTLPLEHSKN
                     PGLVERFLHEAEVASTIAHPHCVTIHDFGQTREGLLYLAMEYLDGQSLEDRLQQAPIS
                     IPEVIEITNQVAAALTAAHAQRIVHRDLKPANIFLLSMPDHSVFVKVLDFGIAKALDS
                     TRRLTGTGEVFGTPWYMSPEQSLGQAVDGRSDLYSLGCILYEMLTGRPPFDADNPMAI
                     LSAHIHTPVPPIDQTTQRSDIPPELARLCMRLLRKSPDERPPDAASARASLKSLGDTA
                     DRDVLPSLSTLAAASTAANSTTIVHDDKAKTQDSSSSASTTEKSLALPWILVLGLIGT
                     IVGVGTIATLWTWLAQGSPDLLVP"
     gene            1549..3015
                     /locus_tag="PK710_15810"
     CDS             1549..3015
                     /locus_tag="PK710_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11246.1"
                     /translation="MTPSNETIRPKFPRPSWPTRQIRHIGPLVLLMGWLGTGCHDDST
                     SDSTTADEPDADAGDEEANEGQDAFQDSDIEPATLDELAASYCSGMAATHCHYAVSCG
                     CTTIPGFPSDCLGRYEMNCRRWIERFRDAVEQGAIVVHLAAVESCVKRYEQLGAHCAL
                     PAADYFVDDCPIFVPRDGFGPRAGIDASCEQAVCEQGLRCGSEYRCVTPGQHNDACAT
                     SADCDPLLICVSGRCTDADTLVAGNDCMSPDACEPGLSCLASAKSVCRETAIGEPCMG
                     DGTCSGESVCATSSDGSQSECTLAAGEAQECLDGVHCATGLACAGGICAPKPQQGQPC
                     ALGLLGPHVCADGLSCVEGACETAPGEGQACGYGEVQCATGLGCAFEGADSLCRPKRG
                     EGSACENDDMCLDELHCEFSIGQCEADLADGQACVNGNECSEGSACLPDEYLEFRCAP
                     IPKNGESCMLDECADKLACRRVAEHGACAPEYCTAFLF"
     gene            complement(3149..3811)
                     /locus_tag="PK710_15815"
     CDS             complement(3149..3811)
                     /locus_tag="PK710_15815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOT11247.1"
                     /translation="MPAQIQWSAVQQYVEGLVPPRHPQLQRMEQEAHKLRFPIIGPAC
                     GQLCYQLARMIGAKRVFELGSGFGYSTAWFARAMSENGQGQVFHVVWDETLSTQAREH
                     LSAMGLDPFVQFRVGEAIAALRDAEGPFDIIFNDIDKEGYPEAFPVIESKLRVGGVMI
                     VDNLIWHGQVLDERDREASTMAIRLFTEQVVKSDRWISNLVPIRDGLLVATKIAEMAR
                     VP"
     gene            3966..4940
                     /locus_tag="PK710_15820"
     CDS             3966..4940
                     /locus_tag="PK710_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11248.1"
                     /translation="MRSSRVSNLFLLALLAVGCKDPATHTTSSNASASSLVPPAPSCG
                     ERMEKMSAWLTTLLQEGAGPTAPLQEANIQLASLEQGELWAWHGAAVIVSSSVLQIDR
                     ERLGSPQESTTSDKLQAAFKARSQSALSAGFSLGIDLHIDENTPWETVVSVHQAGAKA
                     GIRTAGLVFAGASDLEPPTCPVLEELVGTSKTDEPPSDDLLSSEAPSPEQKALESCRP
                     AVSILRDIAKRDLGGKERLEYYVKRAPLAWQACDCRGDERAVRAIAWYWSGRHHGPPT
                     RTIRISLGVKGEKDTMTVSASKTDPWSKVSQSVIEAAQSNRRLVFAVE"
     gene            5019..5606
                     /locus_tag="PK710_15825"
     CDS             5019..5606
                     /locus_tag="PK710_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11249.1"
                     /translation="MDAKGHPQRPLERSSLPIERITDAFLAETKEESPELYKYLGHLD
                     AQGRLELGGVLGRFDFRHKGELDAEQRLMARRVLGRLHRPATSMLVLVNRVLDYLDLN
                     NNALLEPDEVELCVEIFELFAHADSDNDTVSEHELELLYAAIRQMDRDDNHALDALER
                     RELREALQNPKAFLERQRLRNPRVAELMRSRSPSS"
     gene            complement(5631..>6690)
                     /locus_tag="PK710_15830"
     CDS             complement(5631..>6690)
                     /locus_tag="PK710_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11250.1"
                     /translation="EGMKRPWALLAGIIAALHPAFVPYTAALMSDGAASTFCVAAVAL
                     TMATRRAKGKRQMPSAMALGIVLGLGALVRPQMLLCVPFLGWLASAFERNIRKRWLVV
                     LAVGSLSLVTVMPWVVRNQVRMGHAGLSFNGGWNLLIGTDASAHGTYAPLQVPPSCRD
                     VFDEAKKDHCFAKAARERIASDPRAWSSLIPQKLASTFDYCGAAVWYLHDANPTAFPY
                     RAKLIGAGIETAAIRLLWIASVLAVARIKGPRPSMRAAGAVVSVIFFLTPHGWAGVVG
                     LLFMMGLLGKKLATLPIAVPATAIVIGTTMLTHAAFFGAGRYAMMTLALIPSLIKSPT
                     PRNEYDSPSDPWAKASMA"
BASE COUNT         1420 a         1895 c         1935 g         1440 t
ORIGIN      
        1 ccatcagcgg gtttctcgtg ctcataagcc tggtgctgct ccgatgaccc atcgattctt
       61 tgctccacga aagcgattgc gttcacgtat gcccatccgg ttggattacc catgaaatca
      121 agcgtcgagc ccccacgacg ataagctacg atactgccat gaacgacgac acccttccgt
      181 tttccgcaag ccttgcgacc gccaacacga tggtggcaag ggtttgtacg acctgcggaa
      241 aaacttatcc tccgagtgcc gtgtactgtt ccatcgatgg cagcgagcta tccgccatcg
      301 cgaccgacga caccgatccg ttggttggac aagtgctcga cggtcgttgg gtcattcgtc
      361 gacgcattgg tgctggcggg atgggagctg tgtatgccgc ttcgcaattg agcgtgaatc
      421 gtcaggtagc gatcaaaacg ctcccgttgg agcactcgaa aaatccaggg cttgtggagc
      481 ggtttttaca cgaagccgag gtggcgtcga ccattgccca cccgcattgc gtcaccatcc
      541 atgacttcgg tcaaacacgc gagggtttgc tgtacctggc catggaatat ctcgatgggc
      601 agtccctcga ggatcggctt caacaagcgc cgatttccat tccggaggtg attgaaatca
      661 ccaatcaagt cgccgcggcg ctcacggctg ctcatgcgca gcgaatcgtg catcgcgacc
      721 tcaaacccgc caatattttc ctcctctcga tgcccgacca cagtgtcttc gtcaaagtcc
      781 tcgacttcgg gattgcgaaa gcactcgact ccacccgacg actcaccggc acaggagaag
      841 tgtttggcac accctggtac atgagccccg aacaaagcct cggccaggcc gtagatggcc
      901 gatcggacct ttattctctc ggctgcatcc tgtacgaaat gttgaccgga cgtccgccct
      961 tcgacgcgga caatccgatg gcgatcttgt cggcgcatat ccacacgccc gttccaccga
     1021 tcgaccaaac gacgcagcgc agcgatatcc ccccggaatt ggcaagactc tgcatgcggc
     1081 ttctgcgtaa atcgcccgat gaacggcctc ccgatgcggc cagtgcaaga gcttccctga
     1141 aaagcctggg tgacacggcc gatcgtgatg tgctcccctc cctatccacc ctggctgccg
     1201 cttccacggc tgcaaacagc accaccatcg tgcatgacga caaggccaag acacaggatt
     1261 catcatcctc ggcttccacc accgaaaagt cgttggcact tccgtggata ctggttcttg
     1321 ggctcatcgg aactatcgtg ggtgttggca ccatcgccac gctctggact tggctcgccc
     1381 aaggaagccc ggacctgctc gtgccgtgag cggaacaagg cgtctctcca acaaccccgt
     1441 ttttagccca ccctatttgc ggaagtaccc tttctccgct ctcgtaacat cgcgttttta
     1501 ttagttgttc acttgtttgg ccccgcgctc tttgctagct cacctgccat gacgccctcg
     1561 aacgaaacca tacgtcccaa gttcccaaga ccgtcatggc caacaaggca gatacgacat
     1621 attggtccgc tggtattgct catggggtgg ctggggactg gctgtcacga tgactcgacg
     1681 tctgactcga caaccgcgga tgaaccggat gcggacgcgg gtgatgagga ggcgaatgaa
     1741 gggcaagatg cctttcaaga cagcgacatc gaaccggcaa cgctcgatga actcgcggct
     1801 agctattgtt ctggcatggc cgctactcat tgtcattacg cggtgagttg cggttgcacg
     1861 acaattcccg gcttcccgag cgattgcctg ggccggtatg aaatgaattg tcgtcgatgg
     1921 atcgagcgat tccgtgatgc tgtcgagcag ggcgcgattg tcgttcacct cgcggcggtc
     1981 gagtcctgcg tgaagcgata tgaacagctc ggagcgcatt gtgcgctgcc ggctgcggat
     2041 tatttcgtcg acgattgtcc tatcttcgtg cctcgcgacg gctttgggcc acgggcgggg
     2101 atcgatgctt cctgtgagca ggcggtttgc gagcagggac tgcggtgtgg gagtgagtat
     2161 cgttgtgtaa caccgggtca acacaacgat gcttgtgcga cttcggcgga ctgcgatccg
     2221 ctgctgattt gtgtatcggg acggtgtacg gatgcggata cacttgtagc cggtaacgac
     2281 tgcatgtcgc cggacgcatg tgaacctggg ctttcatgcc ttgccagtgc gaagagcgtt
     2341 tgtcgcgaaa ccgccatcgg agaaccgtgt atgggagacg gaacttgctc tggcgaaagc
     2401 gtttgtgcga cgtcttcgga tgggagccaa tcggagtgta cgctggcagc cggcgaagcg
     2461 caggagtgtt tggacggggt tcattgtgcg acagggctgg cgtgtgcggg tgggatatgc
     2521 gctcccaaac ctcaacaagg acaaccttgt gcgttggggt tgttgggacc ccatgtttgc
     2581 gccgacggtc tttcttgtgt cgaaggcgcc tgtgaaacag cccccgggga aggacaggcc
     2641 tgtgggtatg gagaagttca atgcgcgacg gggcttgggt gcgcgtttga aggggcggac
     2701 agcctatgtc ggccgaaacg aggggaaggg tccgcttgtg agaacgatga tatgtgtctt
     2761 gacgaactac attgcgagtt ttcgatcggc cagtgtgagg ccgaccttgc ggacggccag
     2821 gcttgtgtca acggcaacga atgctctgag ggttcggcct gccttccgga tgaatatctg
     2881 gagtttcgtt gcgctcccat tccgaaaaac ggcgagtctt gcatgctcga tgaatgcgcg
     2941 gacaaactcg cttgcaggcg cgttgcggag cacggagctt gcgcgcccga atattgcacg
     3001 gcatttctat tttgaacgag ccgtcgatct cgtgcggccg gtgtcatggc tggagacgat
     3061 acgcgcggat cgagcacgcc atcgccagga tcggtgagag gccatgcccc gtatatcccg
     3121 atagggacta tacgtacgtt accgcgactc agggcacgcg cgccatttcc gcgattttgg
     3181 tggccacgag cagcccgtcg cgaatcggaa ccaggttcga gatccaacgg tcgcttttca
     3241 cgacttgttc cgtgaacaat cgtatggcca tggtcgaagc ttctcgatct cgttcatcga
     3301 gcacctgtcc gtgccaaatc agattgtcga cgatcatcac cccccctaca cgcaacttgc
     3361 tttcgatgac gggaaaagcc tcgggatagc cttccttgtc gatatcgttg aaaatgatgt
     3421 cgaaaggtcc ttcggcatca cggagcgcgg cgatggcctc acccacgcga aactgcacga
     3481 acgggtctag tcccatggcg gataggtgtt ctcgtgcttg tgtggatagc gtttcgtccc
     3541 acaccacgtg aaacacttgt ccctgtccat tttcgctcat cgcgcgagcg aaccatgcgg
     3601 ttgagtatcc gaatccggat ccgagttcga acactcgttt cgccccaatc atccgtgcca
     3661 attgataaca caactgacca caagctgggc cgatgatggg aaagcggagc ttatgagcct
     3721 cttgctccat gcgttgcagt tgtggatgac gaggcggcac caatccctca acatattgtt
     3781 gaacggcgga ccattgaatt tgggctggca tggaagattc cttgaggcgc cggacaaacc
     3841 ccgtcaagcc gtacccctag cgtgttcatg acagccgtgg ctagggcagc ctttgtacga
     3901 aagcgcgttg agaaaagaca gcgtcgggta tccggtctac catggctcca accgcggagg
     3961 tgttcgtgcg atcctcaagg gtatccaacc tatttttgtt ggctctcctc gctgttggct
     4021 gcaaagaccc agcgacccat acgacttcat ccaatgcgtc cgcatcctcg ttggtccctc
     4081 cagcgccgtc gtgtggagaa cggatggaaa aaatgagcgc gtggcttacc acgctgttgc
     4141 aagaaggggc aggtccaacg gcgccgctgc aggaggccaa cattcagttg gcatcgctgg
     4201 aacagggaga actatgggca tggcatgggg cggccgtcat cgtttcctcc tcggttctgc
     4261 agatcgatcg cgagcgcctt ggttctcccc aagagtccac aacgagcgat aagctgcaag
     4321 ccgcgttcaa agcacgttcc caatcggcgc tgtccgccgg gttctctttg ggaatcgatt
     4381 tgcatatcga tgaaaatacg ccttgggaaa cagtggtttc cgtccaccag gcgggagcga
     4441 aggcgggaat tcgaaccgcg ggactcgtat ttgcaggagc cagcgacctc gaaccgccca
     4501 cgtgtcctgt tttggaagaa ctcgtcggaa catcgaaaac ggatgagccc ccgtcggacg
     4561 atctgctttc ctccgaggcc ccaagccctg agcaaaaggc gttggaaagc tgtcgtcctg
     4621 ccgtatccat cctgcgagac atcgcgaagc gagatctcgg tggcaaggaa agactcgagt
     4681 actacgtcaa acgcgctcct cttgcgtggc aagcctgtga ttgtcgtggc gatgaacgag
     4741 ccgttcgcgc gatcgcctgg tattggtctg gacgacatca cggaccgcct acccgaacca
     4801 tccgtatatc gcttggtgtg aagggcgaaa aagatacgat gacggtctcg gcttccaaga
     4861 ccgatccatg gtcgaaagtc agtcaaagcg tgatcgaggc ggcgcagtcg aaccgacgcc
     4921 tcgtatttgc tgtcgagtag ttctctttgc gctggggcaa tcgtacggcc cgatgtaacc
     4981 ttagggctcc ttgcatcggc aatcattgtg gagtcatcat ggacgcaaag ggtcatcctc
     5041 aacgcccgtt ggagcgcagt tcacttccta tcgaacgtat taccgacgct tttctcgcgg
     5101 aaacgaagga agagtcccct gaattataca agtacttggg tcacctggac gcgcaaggtc
     5161 gactcgaatt gggaggagtg ctcgggcgat tcgactttcg tcacaagggt gagttggatg
     5221 ccgagcaacg cctcatggca aggcgcgtgc tcggtcgtct tcatcgacca gcgacgagca
     5281 tgttggttct cgtcaatcgc gtattggatt acctggacct gaacaacaac gccttgttgg
     5341 aaccggatga agtcgagcta tgtgttgaga ttttcgagct tttcgctcat gccgattcgg
     5401 acaacgacac cgtttcggaa cacgagttgg agttgcttta cgccgcgata cgtcagatgg
     5461 atcgagatga caatcacgcg ttagacgctc ttgaacgtcg tgagcttcgg gaagcgctgc
     5521 agaatccgaa agcgtttctc gaacggcaac gcctgcgtaa tccgagagtt gcggaattga
     5581 tgcgttcacg ttctccctcg tcatgagggt agcggaaggc cggattcgga tcaggccatg
     5641 cttgctttcg cccatggatc cgaaggcgaa tcgtattcat tccgtggtgt tggacttttg
     5701 ataagcgagg gaatcaacgc caacgtcatc atggcgtacc ggcccgcacc aaaaaacgcg
     5761 gcatgcgtga gcatggtcgt accgatcacg atggcggttg ctggaacagc gatgggcaat
     5821 gtggcgagtt ttttgcctag taatcccatc atgaacagca agccaaccac ccccgcccac
     5881 ccatgaggcg tgagaaaaaa aatgacgcta acaaccgctc ctgctgcgcg catcgagggt
     5941 cgggggcctt ttatccgcgc cacggcgagc acggatgcga tccacagcag gcgtatcgcc
     6001 gcggtttcga tccccgcccc aatcagcttg gctcgataag gaaacgccgt cggattggca
     6061 tcgtgcaagt accagaccgc agcgccgcaa taatcgaacg tggatgcgag tttctggggg
     6121 atgagcgatg accatgctct tggatcggag gcgatacgct ctcgcgcggc ctttgcgaaa
     6181 caatgatcct tcttggcttc atcgaatacg tcacgacacg agggaggcac ctgcaacgga
     6241 gcgtaggttc catgggcaga tgcgtcggtt ccgatcagca ggttccaccc tccattgaaa
     6301 gaaagtcccg catgccccat acgcacttgg ttgcgaacta cccagggcat caccgtaacg
     6361 agggaaagtg aacccaccgc aagcacaacg agccatcgtt ttcggatgtt tcgttcgaaa
     6421 gcagaagcaa gccaaccaag gaatggaaca caaagcaaca tctgcggtcg aacgagagca
     6481 ccgaggccca acacgattcc cagcgccatg gccgaaggca tctgacgttt tcctttcgct
     6541 cgtcgtgttg ccatcgtcaa cgccacggct gcgacacaga aggtcgacgc ggcaccatcg
     6601 ctcatcaacg cggcggtata gggaacgaag gcggggtgca acgcggcgat gattcccgcg
     6661 agcagcgccc atggacgctt catgccttcg
//
LOCUS       DAOSOR010000316         6672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_11554_length_6672_cov_3.530301, whole genome shotgun sequence.
ACCESSION   DAOSOR010000316 DAOSOR010000000
VERSION     DAOSOR010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11554_length_6672_cov_3.530301"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /gene="mtnA"
                     /locus_tag="PK710_15835"
     CDS             complement(<1..822)
                     /gene="mtnA"
                     /locus_tag="PK710_15835"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOT11251.1"
                     /translation="MDELRMARTLPVEVPISGASYSAVELADENDRVWMMDQRLLPWQ
                     EAYREIRTVERVAEGIRQMVIRGAPAIGIAAAYGMVLAAAACRGDAGDYERVMKQAGD
                     LLRQARPTAVNLGWAVDMVVGTLSQHADLGYEERIRVVAELARRIHEQDVAACKAMGK
                     LGAERIRDGATVLTHCNAGALATGGYGTALGVIRAAREAGKAVHVLADETRPYWQGVR
                     LTAWELHRDGISVEVITDGMAAHHFSQGEIDHVVVGSDRIARNGAVANKIGTYSVA"
     gene            complement(869..2302)
                     /gene="gatB"
                     /locus_tag="PK710_15840"
     CDS             complement(869..2302)
                     /gene="gatB"
                     /locus_tag="PK710_15840"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOT11252.1"
                     /translation="MVTDPGYEPVIGLEIHAQLRTRTKLFCGCLADFGAAPNTRLCPV
                     CMGLPGALPTLNRQAVALAVRAALGLGCRIGARSVFTRKHYFYPDLPKGYQITQGDFP
                     LGTDGTVHLAAGMDSSVLERVIRVERLHLEEDTGKSVHHAEYGLVDLNRAGIPLIEIV
                     TLPDLRSSQEASDCLGEIRNVLMRLEVCDGNMEEGSLRCDVNISVRKSSGELGSRVEV
                     KNLNSFRFIRRAIGYEQARQAEILRSGGQVQPETRGWNEREGKTYTLRSKVSSNDYRM
                     VDEPDLLPLDLSRDWVRKQQAQLPELPYQTRKRYASLGLSRDMVITLHSQKWLMDLFD
                     EVMELGLEPSGIANFLATEVLRDAGQGAPIRAWQVGELMGLVAEGTISGKQMKELYGW
                     VKSTDESPRQVAVERGLRCVADESTVFLVCERVVTSNAQMVHKYRQGKKGLMGYFMAE
                     VMKQTQGSADPRVATAILQRMLEEEQV"
     gene            2595..4406
                     /locus_tag="PK710_15845"
     CDS             2595..4406
                     /locus_tag="PK710_15845"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT11253.1"
                     /translation="MNSLDDIRPGATIGRFEFLVPIAKGGMAAVWAARLKGSRGFSKT
                     VAIKTMLPMLSDDPMFEQMFLDEAQIAALIHHPNVVEIMDLGEQDGILFQVMEYVDGE
                     SLATVLRTQNKKKERIPLEIAVQIIIDACRGLHAAHELKNVDGTLLGLVHRDISPQNI
                     MITYDGVVKLVDFGVAKAAGRTTSETTAGQIKGKAPYMAPEQALGQHVDRRTDVFAMG
                     IVLYQITTGKHPFRGENDIATLHNILHNDLPSPRFVDPQFPRPLESAIHRALSRDITQ
                     RFASAKDMADALSRVFPPTRRRTDVADVGTFVQNLLGESGNRRRMALAEAIKRADLRT
                     SSTPLSEASGPILLPLAELGTDSAIHSPRGLAAPTERIDVGDLSFNRPSQQPHPPNHE
                     LPPPPDAHDPIRTAQTIRPGSGEYAVVSSPSDDPLPHFDDEILANNGLNLIDSSGSIP
                     TTDSSPPPKSRWPKTAFILGTTILGIGAGITGWVVLQPQSPPATAPTASTNVSPEAAP
                     IATAPIADDAPADYTIDALSSAAPEDADTPPTMPPSSNVWIPKSFPPSHPHPTTTAVS
                     VAPSATPPKPTAAPTSTAPTTTATSTWKPPPVSDPGF"
     gene            complement(4437..5969)
                     /locus_tag="PK710_15850"
     CDS             complement(4437..5969)
                     /locus_tag="PK710_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11254.1"
                     /translation="MGNYRSTRMLLLMVLGAVGCAYPDYSYVDMVDGASGTGGTGGGN
                     IGGAAGASGTGGTAGASGTGGGAGSSGTGGTAGASGTGGTAGISGSGGVAGAAGGGGE
                     GGVQEGGTDVEEDVDSSVEADVIEADVVEADVVEADVVEADVIQDVTQEEVSVDTGPE
                     TGECPTTVENCTNGIDDNCDGKVDCEDPQCSPLYRCVPPVPAGWNGYYSLFDGSTSTK
                     TPDCLSPFSTLGLVGYRSPLFEQASCASCFCGSPSGVKCQEPWVAIFVDPTCSAGAYW
                     HLSDPSENCGEPGHPRHCDMQPHGVCKGANLNPGDPPVYPSAVDFQVGQPVAGTGACT
                     EYGGGVISEPPAPSWQRIGLSCQSDSGMSGAGCSNAYACVPKSSEPFLSGTCVYKEGA
                     VDCPNTQFSKKYVYYLDWVDTRGCAECKCTIPSVTSCEGRLTVALDNTCPTTGGNAEA
                     YITMSSNEPYRCEPITSAETHYLRYEDLGVTGQCDPSPSMPIGGVVGNGNTAFTFCCL
                     QL"
     gene            complement(5986..>6672)
                     /locus_tag="PK710_15855"
     CDS             complement(5986..>6672)
                     /locus_tag="PK710_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11255.1"
                     /translation="AQETLTEAQAGIAEVEAKIPKLTIELGAGAEAASVTLDGVALGD
                     ASVGKEVPVNPGAHTIRYTLPGGKVHQRIVRLKEGESKKVVLADSAADVVEPDTKDDA
                     SVRTEDSGSNVLPWVMIGVGGASLVTSGVFYLMRSSTIQDHEDQCVGNVCPRSLEDTG
                     DKGKTYGMVGNITLGVGLVGVGVGTIMLLAGGSSSTETAGQTGQAASNLTVLIGGSGR
                     GAEAALVGTF"
BASE COUNT         1544 a         2443 c         1355 g         1330 t
ORIGIN      
        1 cgccacactg taggtcccta tcttattcgc tacagcccca ttacgggcaa ttcgatcgga
       61 ccccaccacc acatggtcta tctctccctg ggaaaaatga tgcgccgcca tcccatcggt
      121 gatgacctcg accgatatcc cgtctcgatg aagctcccac gccgtcaaac gaacgccttg
      181 ccaatacgga cgagtctcgt ccgcaagaac atgcaccgcc ttgccagcct ctcgcgccgc
      241 tcgaatcacc cctagcgccg taccatatcc ccccgttgcc aacgctcccg cattgcaatg
      301 ggtaaggacg gtcgccccat cccgaatgcg ctccgctccc aacttgccca tcgctttgca
      361 tgccgccaca tcctgctcgt gaatacgtcg cgccaattcc gccactaccc gtatccgctc
      421 ctcataccct aaatccgcat gctgcgacaa agtccccacc accatgtcca ccgcccaacc
      481 caaattcacc gccgtgggac gcgcttgccg aagcaaatcc cccgcctgct tcatcacacg
      541 ctcgtagtcc cccgcgtcgc cgcgacaagc tgccgctgcc aataccattc catatgccgc
      601 cgcaattcca atcgcaggcg ctccccgtat caccatctgg cgaatgccct ccgccactcg
      661 ttcgacagtc cgaatctccc tatacgcctc ctgccaagga agaagacgct gatccatcat
      721 ccacacccga tcgttttcat ccgccaattc gacagcggaa taactcgctc ccgaaatcgg
      781 aacctcgacc ggaagtgtcc tggccattcg caattcgtcc atttttttac ctgttgccgc
      841 aagaaaagcc aaggtcaagc ctttcggact acacctgctc ttcttccagc atccgttgca
      901 aaatcgcggt agctacacgc ggatccgcac tgccttgggt ttgcttcatc acctcagcca
      961 tgaaatatcc catgagccct tttttgcctt ggcgatactt atgcaccatc tgcgcattcg
     1021 aggtcaccac acgttcacat accaaaaata cggtggactc atccgccaca caccgcaatc
     1081 cccgctctac cgcaacctgc ctgggcgact cgtccgtgct ttttacccac ccatacaatt
     1141 ctttcatctg cttgcccgaa atcgttccct ccgccacaag ccccatcagc tccccaacct
     1201 gccaagcccg aatcggagcc ccctgcccag catctcgaag aacctccgtc gccaaaaaat
     1261 tcgcaatacc actcggctcc aaccccaatt ccatcacttc atcaaataga tccatgagcc
     1321 acttctgcga atgcaacgtg atgaccatgt cacgagacaa accaagcgac gcataacgct
     1381 ttcgcgtttg atagggaagc tccggcaatt gcgcctgctg ctttcgaacc cagtcccgcg
     1441 aaaggtcgag cggcaacaaa tcaggctcat ccaccatccg ataatcgttc gagctaacct
     1501 tggaacgcaa cgtatacgtc ttgccctccc gttcgttcca ccctcgcgtc tccggctgta
     1561 cctgcccgcc gcttcgaagt atctcagcct gccgagcttg ctcataacca atcgcccgac
     1621 gaatgaaacg aaacgaattc aggttcttca cctccacccg cgatcccaac tcacccgaag
     1681 acttccgcac cgatatgttg acatcacatc ggaggcttcc ctcctccatg tttccgtcac
     1741 atacctcgag acgcatcaac acgttgcgaa tctcacccaa acaatcagaa gcctcctggc
     1801 tcgaacgcaa atcgggcaag gtcacgatct cgatcaacgg aatcccagca cggttcaaat
     1861 ccaccaaacc atattcggca tgatgaacgc tcttgcccgt atcctcttcg agatgcaaac
     1921 gctcgacccg aatcacccgc tccagaacgg aactatccat tcccgcagcc aagtgaaccg
     1981 ttccgtctgt ccctaacggg aaatcgcctt gtgtgatttg atatcccttg ggcaaatccg
     2041 gataaaagta atgttttcgc gtaaatacac ttcgcgcacc aattcgacac cccaacccga
     2101 gggcggccct aaccgccaac gctaccgctt gccggttgag tgtcggtaac gccccgggca
     2161 accccatgca aacgggacac aagcgcgtat tgggcgccgc cccaaaatct gccaaacatc
     2221 cgcaaaataa cttggtccgg gtccgaagct gcgcgtgaat ctcgagacct atgaccggtt
     2281 catacccagg atcggttacc atacccatcc aaccacccaa caacagcact cgcgaaccat
     2341 gattttttct ttccccacgg gatgcttctc acctacaaga taaaaaaaag atgggggata
     2401 ttgctcaagc caatttctcg tttttttcat tgcttgccca taaccaagca ccgacgcccg
     2461 ctcaatacga gatgaaaata catactgagt gcggacgcat accatcgaaa ccgtcgacca
     2521 aaccgaaaaa aggaatgcct acccccttgt gtcaatgcca aacctttggg agtctgcttg
     2581 gaacctgagc cctcatgaat tccctcgatg acatccgccc cggggcaacc attgggcgat
     2641 tcgaattcct cgtccccatc gcgaaaggcg gtatggccgc cgtatgggcc gctcgcctca
     2701 aaggctcccg cggattctcc aaaaccgtgg cgatcaaaac catgctcccc atgctctcgg
     2761 acgatcccat gttcgagcag atgttcctcg atgaagctca aatcgccgcg ctcatccatc
     2821 atcccaacgt cgtcgaaatc atggacctcg gtgaacaaga cggaatcctg ttccaggtca
     2881 tggagtacgt cgacggagaa tcgctagcaa ccgttctccg tacccaaaac aaaaaaaaag
     2941 aacgaatacc ccttgaaatt gccgtccaaa tcatcatcga tgcctgccgc ggtcttcacg
     3001 ccgcccacga actcaaaaat gtggacggca cccttctagg cttggtccat cgggacatct
     3061 ccccccaaaa catcatgatc acgtacgacg gcgtggtgaa actcgtggac ttcggtgttg
     3121 ccaaagccgc gggacgcacg accagcgaaa ccaccgcagg ccaaatcaaa ggcaaagcgc
     3181 cctatatggc cccagaacaa gcccttggac aacacgtgga tcgcagaacc gacgtgtttg
     3241 ccatgggtat cgtcctgtat cagatcacca caggaaaaca cccgttccga ggcgaaaacg
     3301 atatcgcaac cctgcacaac attctgcata acgaccttcc ctcccctcga ttcgtcgatc
     3361 cgcaatttcc tcgtcccctc gaatccgcca tccatcgcgc cctttctcga gatatcaccc
     3421 aacgtttcgc ttccgccaaa gatatggccg atgccctcag ccgtgtattc cctcccaccc
     3481 ggcgccgtac cgacgtagcc gatgtcggca ccttcgtgca aaacttgctg ggagaatcag
     3541 gcaaccgacg acgcatggcc ttggctgaag ccatcaaacg cgctgacctg cgaacgagtt
     3601 caactcccct gtccgaagcc tccggcccta tcttgctgcc tctggccgaa ctcggcaccg
     3661 actctgcgat ccatagccca cgaggactcg ccgcccccac cgaacgcatc gatgtcggcg
     3721 atctcagctt caatcgccca tcacaacaac ctcaccctcc gaaccatgaa ttacctcccc
     3781 ccccagacgc acacgatcct atccgcaccg cgcaaaccat tcgccccggc tccggcgaat
     3841 acgctgtcgt atcttctccc agcgacgatc ctttgcccca tttcgatgac gaaattctcg
     3901 ccaacaacgg gctgaacctt atcgactcct ctgggtccat ccccaccacc gattcgtcac
     3961 cacctcccaa atcccgttgg cccaaaactg ccttcatcct cggaaccacc atcctcggca
     4021 tcggcgcggg aatcaccgga tgggtcgtcc ttcaaccaca atcccctcct gccaccgccc
     4081 caaccgcatc caccaacgtc tcacccgagg cagccccaat cgcgaccgct cccatcgctg
     4141 acgacgcccc ggctgactac acaatcgatg ccctatcttc cgctgctcct gaagacgccg
     4201 atactccacc gacaatgcca ccgtcttcca acgtgtggat acccaagtca ttccccccat
     4261 cacacccgca ccccaccacg accgccgtat ccgtagcccc ttcagctacc cctcctaaac
     4321 ctaccgctgc cccaaccagc accgcaccca cgacaaccgc cacgtccacc tggaaacctc
     4381 cccccgtctc tgacccaggt ttctaacggc tgccctgcct caaaacggca aatgcctcag
     4441 agctgtaaac aacagaaggt aaacgctgtg ttcccgtttc cgacaacccc tcctatcggc
     4501 atgctcggcg acggatcaca ctgccccgtc acccctaggt cctcgtatcg taaataatgg
     4561 gtctcggcgc tggttatcgg ctcacaacgg taaggttcgt tcgaggacat ggttatataa
     4621 gcttccgcat ttcctccagt cgtaggacac gtattgtcca aagcaaccgt cagcctccct
     4681 tcgcaactcg tcacggaagg aatcgtacac ttgcactccg cgcatccccg cgtatccacc
     4741 caatcgagat aatagacata tttcttcgaa aactgggtat tgggacagtc aacggcccct
     4801 tccttgtata cgcacgtacc cgatagaaac ggctcggatg acttcggcac acaagcatac
     4861 gcattgctac agcccgcgcc cgacatcccc gagtcggact ggcaactcag cccaattctt
     4921 tgccacgaag gtgcaggagg ctccgaaatc accccacctc cgtactctgt gcaggcgccc
     4981 gtccccgcga caggttgtcc aacctgaaag tccaccgcag aaggatagac aggcggatcc
     5041 cctggattga ggttggctcc cttacacacg ccatgcggct gcatgtcgca gtgcctcgga
     5101 tgacccggtt ctccgcagtt ctccgaagga tcactcaaat gccagtaagc acccgccgaa
     5161 caagtcggat ccacgaaaat cgctacccat ggttcttgac acttgacccc cgaaggagaa
     5221 ccgcaaaagc acgacgcgca ggaggcttgc tcaaacagcg gcgagcgata tcccaccagc
     5281 ccaagcgtgg aaaacggaga tagacaatcg ggtgtttttg tactcgtgct cccatcaaat
     5341 aacgaatagt agccattcca ccccgcaggc acgggaggaa cacagcggta caacggcgaa
     5401 cactgcggat cctcgcaatc cacctttcca tcacagttgt catcaatccc attcgtgcag
     5461 ttctctacgg tcgtcggaca ttcacccgtc tcaggacccg tatccaccga cacttcctcc
     5521 tgcgttacgt cctgaatcac atccgcttcc acgacatccg cttccacgac atccgcttcc
     5581 acgacatccg cttcgataac atctgcctcg acagagctat ccacgtcctc ctctacgtct
     5641 gtcccacctt cttgcacacc accctcccca ccgcccccgg ctgctcccgc cacaccacca
     5701 gaccctgata ttcccgctgt cccgccggtt cccgaagcac ctgccgtacc accggttccc
     5761 gatgatcctg cccccccacc ggttcccgaa gcacctgccg taccaccggt tcccgacgcg
     5821 cctgccgctc cacctatatt gccccccccc gtgcctccgg tgcccgatgc gccatcgacc
     5881 atatcaacat acgaataatc aggataggca caccccacgg cacccaacac catgagcaaa
     5941 agcattcgcg tgctacgata gtttcccatc tctactcgct tctctttaga acgtcccaac
     6001 aagagctgcc tccgctcccc ggccacttcc gccaatcaac accgtgagat tgctcgctgc
     6061 ctgccccgtt tgccccgccg tttccgtcga tgatgaacca cccgccagga gcatgatcgt
     6121 tcccacacca acgccaacca accccacccc caacgtgata ttgcctacca taccatacgt
     6181 cttgcccttg tctcccgtgt cttcgagaga ccgcgggcaa acgttgccaa cgcactggtc
     6241 ttcatggtct tggatcgtgc tcgaacgcat caagtaaaat acgccgctcg tgacgagact
     6301 cgcccccccc acgccaatca tcacccacgg aagaacattg gaaccactgt cctcggtgcg
     6361 aacggaagca tcatccttgg tgtcaggctc cacgacatct gccgctgaat ccgcaagcac
     6421 cacctttttg gactcaccct ctttcagacg aacgatacgt tgatgcactt tgcctcccgg
     6481 caatgtgtat cgaatggtat gagcccccgg attgaccggc acttccttac ccacggatgc
     6541 atccccaagc gccacaccat ccaacgtgac agaagccgcc tccgctcccg caccaagctc
     6601 aatcgtcaac ttgggaatct tcgcctctac ctccgcaatt cctgcttgcg cctccgtcag
     6661 cgtttcctgt gc
//
LOCUS       DAOSOR010000317         6661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_11583_length_6661_cov_4.723887, whole genome shotgun sequence.
ACCESSION   DAOSOR010000317 DAOSOR010000000
VERSION     DAOSOR010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6661
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11583_length_6661_cov_4.723887"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..2636
                     /locus_tag="PK710_15860"
     CDS             150..2636
                     /locus_tag="PK710_15860"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOT11256.1"
                     /translation="MALSYRPFRDSLTSILALSVAVFSLACGGDDDASPGPHFPDSGT
                     GGFNPGDGGGGYSGSVDGGDGSTSAGSAGVGGSAGAAGQAGGPSCEDPFKRCPHDFTY
                     PASQESSVELRGSFSATGWDVGIPLTKQGSQWSATVDIPRNFTIRYKYLVDGTTWTPD
                     PGNTNTEPDGQGGVNSLVPGVECEDFTCATEPAPWDCPVDTRTCTTRFIYPVGSETLV
                     QVAGSMNGWQPQAMNKQATSWVLSFPDQAWGDQIEYKFVVDGNWIADPYNPNKTDDGF
                     GGFNSVLGVSCDWWTCGAGSQQCGDVTAFDWRDAVLYFAMVDRFRNSDGKADPVPGAT
                     GDNGHGSSGQVFGGDLNGVTQSLDYLKGLGVSALWLSAPFENRNLPGKGMNDNYQYSA
                     YHGYWPSPEDIDYSKTPPSPLPKVESRIGTSADLKALVSAAHATTTANGHEMKVLFDY
                     VMNHVDIESGLYKARVGQGWFAQHDGHTVLCSGDAVCGGECWDNDPWGMKCAFTDYLP
                     AFDFDNTNARAWSINDAAWWAKEYGIDGYRLDAIKHVPLSWLTELRQRLTAEFPNPAG
                     GRFYLVGETYTWDDYGVLKKYVNPNTMLDGQFDFPFRKQVCRAVLSRESSLQDFSSWM
                     AQNDNRYGPGALMSTWIGNHDIPRVIHAAAGQAGCEEGSHTGNAWTPNYNQPSDAAPY
                     ERLAVAFAIMLTNPGLPMIYYGDEIGLAGGGDPDNRRPLPWNDNQLNTHQKNLRAMVG
                     KLATIRGENKALTRGARSTLYVDADTWVYRMGGCTPDANAVVVAVHRGDGPKTVTVPS
                     RNYTDLWTGAGHAGGSISLSARGFILLRED"
     gene            2921..3943
                     /locus_tag="PK710_15865"
     CDS             2921..3943
                     /locus_tag="PK710_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11257.1"
                     /translation="MKQISSLLWSLGFIAIGLSLGAVACGDDEDDNRPSGSGGATGGA
                     GGATGGSAGETGGVGGATGGSAGATGGSAGETAGAGGATGGSGGGGSQAEFVIGVDGI
                     AAFDAMSSADKDALKGTKVFFQHMSVGENLLMGYNNWSPPWKGGTASLGFVFSSVNDA
                     SSYGSVELGDKAFGYNGNPFNKIKGFREHVLDMGIGSAVQVAGFKFCYNDLTVDITST
                     GTDVIDAYRSAYQDIEKATSGTSFFHVTTPLQPADQWQTVENNTLRRSFAEFLRSTYA
                     GGRHVVFDLQQIESTDTNGSTCSQSGVPVLCEAWAADSDGHLNDAGATRAAKAFLYAL
                     HVARGL"
     gene            4158..5711
                     /locus_tag="PK710_15870"
     CDS             4158..5711
                     /locus_tag="PK710_15870"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HOT11258.1"
                     /translation="MSAQGKGRYRCSTTVGKVFLISATILGVTVAFSCRLGESLDDFT
                     KGGAGPKGGAGGGAGGSGGSGGTGGTSAGGGSGGSGASAGTGGLDVFCDTAPSQCEDL
                     GNEGEQLFGCCEDNIRWWCEKVEQKWNLRSENCDMTGSQCRYDSQVKAMRCVAGQGGT
                     GGSGVCESGECTGSCQNCDKESPDCETDTNTNPDHCGKCDHSCQGGKCVGGKCQVSVL
                     AASQKTPWGIAVDDTTVFWLAEDKSDGSVYSIPKGGGTAQKLASGQNGPVQVAIDDGF
                     VFWTNFQGGGSIRRVKKNGTDLTTLAQASGPWAIALNNDNVYWTNTSDGSIRRVVKPG
                     GQPVVLVTGESSPRGIAIDEKQMYWTTSTGGQVRRANLDGTSPDSLVAGQNYPLGIAV
                     DDTWVYWTEVGASYSLGDCGQADGRIARAKKADGSSHTTLASNQACPLNLVVKDGLVS
                     WVNAGTVTGSNYNYDGTIQQANTDGSDAKLVATGQDRPYGIAVGDNAIFWTNQGVYAG
                     QGSVMKIAR"
     gene            5976..>6661
                     /locus_tag="PK710_15875"
     CDS             5976..>6661
                     /locus_tag="PK710_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11259.1"
                     /translation="MPSKDAGRDTATDVKPDTNVTDSAQDTVTEETGPTECEPKTCAQ
                     LEANCGSAPDGCGEKIECGECPDGQLCGGGGPNVCGTEACYPKSCVQVGAQCGWASDG
                     CSLAIDCGECSPPETCGGGGEMNVCGCSPKTCSQLGANCGTLPDGCLGTLNCGSCSGG
                     QVCGGAGPNLCGSSECMPKTCSQLGVGCGIVSDGCSEALDCGSCESPEVCGGGGIANQ
                     CGCTPKSCSQL"
BASE COUNT         1429 a         1761 c         2075 g         1396 t
ORIGIN      
        1 atttttatcc agatcccaca cccggatttt tcgttttcgt tctcccatcg cctttcgaga
       61 tgggcttggc cttgaaagtg acttacaaat gagtcactgt tcttacgtcg ccgtgtatcg
      121 cccgattcac ctccccaaag gagccaacca tggccttgtc ctatcgccct tttcgtgatt
      181 cgttgacttc gatcctcgct ctatccgtgg ccgttttctc cttggcttgt gggggagacg
      241 acgatgcttc cccaggccca cactttccgg acagtggcac gggggggttc aaccctggag
      301 acggcggcgg aggctatagt ggttcggtcg atgggggaga cggttcgacc agcgcggggt
      361 ccgccggggt gggcggaagc gcgggcgcgg cggggcaagc gggtggccct tcctgcgaag
      421 accctttcaa acgctgcccc cacgacttca cctaccccgc aagccaagaa tcatcggtcg
      481 agcttcgcgg aagcttctcc gccacgggat gggatgtggg cattcccctg accaaacaag
      541 gctcccaatg gtccgccacc gtggatatcc cacgcaactt caccatccgg tacaagtatc
      601 tcgtcgacgg caccacgtgg acgcctgacc cgggcaatac caacaccgag cctgacggcc
      661 agggaggcgt caattcgttg gtgcccggtg tcgaatgtga ggattttacc tgcgctacgg
      721 aacctgctcc ctgggattgt cccgtggaca cgcgcacctg caccactcgc ttcatctatc
      781 cggtgggcag cgaaaccctc gttcaagtgg ccggttccat gaacggctgg cagccccagg
      841 cgatgaacaa acaggctacc tcatgggtat tgagttttcc cgaccaggcc tggggcgacc
      901 agatcgaata taaattcgtg gtggatggca attggatcgc ggacccgtac aatccgaaca
      961 agacggacga cgggtttggg ggattcaact ccgtgctcgg cgtgagttgc gattggtgga
     1021 cctgtggggc gggttctcaa caatgtggcg acgttacggc gttcgactgg cgcgatgccg
     1081 tcctttactt cgcgatggtc gaccgattcc gcaacagcga tgggaaagcc gatccagtgc
     1141 ccggagccac gggagacaat ggtcatggct cgagcgggca ggtttttggc ggtgacctca
     1201 atggcgtcac ccaatccctc gattacctca aaggtctcgg cgtttctgcc ctgtggctct
     1261 ccgctccttt cgagaatcgc aacctgcctg gaaaggggat gaacgacaac tatcagtact
     1321 ccgcctacca tggctattgg ccctctcctg aggatatcga ttattcaaaa acccctcctt
     1381 cccccttgcc caaagtcgaa agccgcatcg gaacaagcgc cgacttgaag gcgctcgtga
     1441 gcgcggcgca tgcgacgact acggccaatg ggcatgaaat gaaggtcttg ttcgattatg
     1501 tgatgaacca cgtcgacatc gaaagcgggt tgtacaaggc gcgcgttgga caggggtggt
     1561 tcgcacaaca cgatgggcat acggtgcttt gctccgggga tgccgtatgc ggaggtgagt
     1621 gctgggacaa cgatccatgg ggtatgaaat gcgccttcac cgactatctg cctgcttttg
     1681 acttcgacaa caccaacgcg cgggcatggt cgatcaacga tgctgcctgg tgggccaaag
     1741 aatacggaat cgatggctac cgtctcgatg ccatcaagca tgtgccgttg tcatggctta
     1801 ccgaattgcg ccagcggttg accgccgaat tccccaaccc ggcgggagga cgattttacc
     1861 tggtgggtga gacgtatacg tgggacgact acggcgttct caaaaaatat gtgaatccga
     1921 ataccatgct ggacgggcag ttcgacttcc ccttccgcaa gcaggtttgc cgagctgttc
     1981 tttctcgaga gtcgtcgctt caagacttca gttcttggat ggcgcaaaac gacaaccggt
     2041 acggcccggg ggcgttgatg tccacctgga tcggtaatca tgatattccc cgcgtgatcc
     2101 atgccgccgc gggacaggcg gggtgcgagg aaggaagcca taccggcaac gcatggacgc
     2161 ccaactacaa ccagccttcc gatgcagccc cttacgaacg tctcgcggtt gcttttgcca
     2221 tcatgcttac caatcccgga ctgcccatga tctactacgg tgacgaaatc ggcctggcgg
     2281 gtggaggaga cccggataat cgtcgccccc tgccctggaa cgacaaccaa ctcaacaccc
     2341 atcaaaaaaa cctgcgggcg atggtaggaa aattggccac gatccgaggg gaaaacaagg
     2401 ctctgacccg tggtgcccgt tcgactttgt acgtcgatgc cgatacgtgg gtgtatcgta
     2461 tggggggctg cacgccagat gccaacgcgg ttgtcgtggc agttcatcga ggggatggtc
     2521 ccaagacggt aacggttcct tcacgaaact atacggattt gtggacgggc gccggacatg
     2581 cgggcggatc gatttccctt tccgcccgtg gattcatcct gctgcgtgag gattgagacc
     2641 gcgatagctg cttgcgacac cccggccata ttcatattta actagcaaaa tcaatacatt
     2701 acagaataag ccccctctct tttctccctt tcgatccttg ctgaaaaccc ttgacgacga
     2761 actgccgacg tcacccgttt cacggtagaa aggaatcggg tcttggtttg cgctttggct
     2821 atcgtgcggg ctcaagcact gacaaagacg agaaagcgtg tggcaaggct ttcgaatgca
     2881 cccgttgcac aaggtatcgt tcggctctac gttcgacgtc atgaaacaaa tttcgtcttt
     2941 gctctggagt ctggggttca tcgcgattgg cttgtctttg ggggccgtgg cttgtggcga
     3001 cgatgaagac gacaatcgtc cgtctggttc tggtggagcc acgggaggcg cgggcggcgc
     3061 cacgggcggt tctgctggcg aaacgggagg tgtgggcggg gccacaggcg gttcagcggg
     3121 agctacaggc ggttctgctg gcgaaacagc aggtgcgggc ggggccacag gaggttcggg
     3181 gggaggcggc agccaagccg aattcgtcat cggtgtcgat ggaatcgcgg catttgacgc
     3241 catgtcctcc gctgacaagg acgcgctgaa aggcacgaaa gtattctttc agcatatgtc
     3301 tgtcggtgaa aacctgctga tgggatacaa caattggtct ccgccgtgga aaggcggcac
     3361 ggcttctttg ggtttcgtgt tcagcagcgt caacgacgcc tccagttacg gtagcgtcga
     3421 gttgggcgac aaagcgttcg ggtataacgg caatccattc aacaaaatca agggatttcg
     3481 agagcatgtt ctcgacatgg ggataggaag cgccgttcag gtggcggggt tcaagttctg
     3541 ttacaacgat ttgacggtgg acatcacatc cacgggcacg gatgtcatcg atgcgtacag
     3601 gtccgcctat caggacattg agaaggcaac gtcgggtaca tcatttttcc atgtcacgac
     3661 gccgttgcaa ccggccgacc agtggcaaac ggtggaaaac aacacgcttc gccgatcatt
     3721 tgccgagttt ttgcgatcga cctacgccgg agggcggcac gtggtattcg atttgcagca
     3781 gatcgagtcg acggacacca atggttcgac gtgcagccag agtggggttc cggttctctg
     3841 cgaggcgtgg gcagcggata gcgacgggca cctcaacgac gcgggggcaa cgcgggcagc
     3901 gaaagcgttt ttgtatgcgc tgcatgtcgc acgcggactg tagcaacgat tttctcgata
     3961 ttctctaacg acagcagcgt gacggcggca gtaccccagc aaacaacctg cccagaaaaa
     4021 ctgtaacgtc cgcatcgcct gacagggctt gttttcgttg tgatcggcca gcggagatag
     4081 gctcgagcgt tatcgggtcg ttatcgttgg tctcgcgttt cttgacggct tgcgattcta
     4141 tttggatgct atgagttatg agtgctcaag gcaaaggccg gtatcggtgc tccacaacgg
     4201 tcggtaaagt attccttatt tccgcaacca tcctcggagt gaccgtagcg ttctcttgtc
     4261 ggcttgggga gtcgctggat gactttacga aaggcggagc tggaccgaaa ggcggagcgg
     4321 ggggtggggc aggcggttcg ggaggttcgg gcgggacggg aggtacgagc gcggggggtg
     4381 gctctggagg cagtggtgca tcggcaggga caggaggatt ggatgttttc tgcgatacag
     4441 ctcccagcca atgtgaagac ttgggcaacg aaggggaaca gctctttggt tgctgcgaag
     4501 acaatatccg atggtggtgc gagaaggtcg aacagaaatg gaacttacgt tccgaaaact
     4561 gcgacatgac cggctcccaa tgtcgttatg actcgcaagt gaaagcaatg cgatgcgtag
     4621 ccgggcaggg cggcacggga ggctctggtg tctgtgaatc aggagaatgc accggatctt
     4681 gccaaaattg cgataaggag tcgccggatt gcgaaacgga taccaatacg aacccagacc
     4741 attgtggcaa gtgcgaccac tcgtgtcagg gaggaaagtg cgtcggaggc aaatgccagg
     4801 tgtcggtact cgcggcttcc caaaaaacgc cctggggaat tgcggtcgat gacacgaccg
     4861 tgttttggct ggcagaagac aagagcgacg gctctgtgta ttcgatcccc aaaggcggag
     4921 ggaccgcgca gaagcttgct tcaggccaaa acggacctgt tcaagtcgcc atcgacgacg
     4981 gcttcgtttt ctggacgaac ttccaagggg gcgggtcgat tcgtcgtgtg aaaaaaaatg
     5041 gtacggatct cacaacgttg gcacaagcca gcggcccttg ggcgatagct ctgaacaacg
     5101 ataacgtgta ctggaccaac acctcggacg gctcgattcg gcgtgtcgtc aagcctggag
     5161 gacaaccggt ggtcctggtc acgggcgagt cgagtccgcg cggcatcgcg atcgatgaaa
     5221 aacaaatgta ctggaccaca tccaccggcg ggcaagtacg tcgggcgaac ctcgatggca
     5281 cgagccctga ttcgctggtg gccggccaaa actatcctct tggcatcgcc gtggacgaca
     5341 cttgggtcta ttggacggaa gttggagcga gttactcgtt gggggactgc ggtcaagcgg
     5401 acggaaggat tgcgagagcc aaaaaggcgg atggctccag tcacacgacc ctcgcttcca
     5461 accaagcgtg tccactcaat ttggtggtga aagacgggct tgtgtcctgg gtcaacgccg
     5521 ggacggtcac cggttcgaat tacaactacg atggcacgat acagcaggcc aataccgatg
     5581 ggtcagatgc caagctggtc gcgacaggtc aggaccgccc atacggaatt gccgtgggcg
     5641 acaacgccat tttttggacc aaccaaggcg tgtatgccgg gcaagggtcg gtcatgaaaa
     5701 tcgcccggtg acgcaagccc ctgtcgcaca cgagcgtttg gcacgaatgc aaatcttcaa
     5761 atctggagct tgagactccc gaacgcaacc cgtatcctta tcgtatgttg ggattattgc
     5821 gctcgatagt gggctcgaag gggaaacaac gcgaggccaa tggatttgtc tttccgttat
     5881 tgaaggatga accagcggtc ctgtccgttt tgctgatcct cctcgcaatg ggcgtggtgg
     5941 tggcaggttg tggaagtgac gatagtacct cttcggtgcc gagcaaagac gcgggtcgtg
     6001 acacggcaac ggatgtgaag ccggacacga acgtcacgga ctcggcgcag gataccgtga
     6061 ccgaagagac gggtcctacg gaatgcgagc caaagacctg tgctcaacta gaggccaact
     6121 gcggttcggc tcctgacggt tgtggagaaa agatcgaatg tggagagtgc cccgacggcc
     6181 agctttgcgg tgggggggga ccgaacgtat gcggaacgga agcgtgctat ccgaaaagct
     6241 gcgtacaagt gggagcccaa tgcggctggg cttctgatgg gtgttctctg gctatcgatt
     6301 gcggcgaatg ctcgccaccc gagacatgtg gcggtggggg cgagatgaac gtatgcggct
     6361 gctcaccgaa aacctgctct cagcttggag ccaactgcgg aacgctgccc gatggttgtc
     6421 tcggaacgtt gaattgtggt tcttgtagtg gcgggcaagt atgtggaggc gcggggccca
     6481 acctatgtgg ttcatcggaa tgcatgccca aaacgtgcag tcagcttggc gtgggatgcg
     6541 gaatcgtctc cgacggctgc agcgaagcgc ttgactgcgg tagttgcgaa agccctgaag
     6601 tatgtggggg tggagggatt gcgaatcaat gcggatgcac tcccaagtca tgttctcaac
     6661 t
//
LOCUS       DAOSOR010000318         6608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_11715_length_6608_cov_4.260644, whole genome shotgun sequence.
ACCESSION   DAOSOR010000318 DAOSOR010000000
VERSION     DAOSOR010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6608
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11715_length_6608_cov_4.260644"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>213)
                     /locus_tag="PK710_15880"
     CDS             complement(<1..>213)
                     /locus_tag="PK710_15880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11260.1"
                     /translation="NRIAQWTPEATRELATEWVRLNLCSWEAFADRIASWKPGASLEQ
                     ATGWVDAGLATWKDFELNLPRWSESAE"
     assembly_gap    214..223
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<224..2962)
                     /locus_tag="PK710_15885"
     CDS             complement(<224..2962)
                     /locus_tag="PK710_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11261.1"
                     /translation="MRKLRVYDRERWHSAGVRVKKLAALDQPVFEARLNRGDRLVFTL
                     RSAQSDRFDGIHIVLWYAGHHDDAVRTAVRRMGIGQRAIDALVDDQPWDESLWGGDNQ
                     KQIQLVTPDGATRRDLPPLVEFSEYEMDRWAESQIDPLLHLSPAQRELTRVAEDRPVF
                     LRGGVGTGKTTVLLYRMLKLLLAGSIENPVFMTFSKSLATWCRSIFERLPGAERRKVE
                     FTSLSGWLRAHFGATRGGKRLFRLAWEKAKLPSNPEPAWWELQRLRGSAAFQGEQPTR
                     GDQLPSHVSPEIQIAYDAYRKLLNGAPDVLDLAWKAYDYYRAESALMNLPFDAVFIDE
                     AQDLTPIEWLVCILMGNRPKLAFFCGDETQDVRDTRFSWEGVEAALRLVGHRAPAFSF
                     FQLHENRRNSEPIALVLQRLAERFDLGATTHAGSEEGPSPLVCVTSRKIAEQTAQRAG
                     CPVLLDLEAREERSPNPFFWVLDLEHIKGLEFHVLPLWAPAGSWPSSPHLARKLFTAI
                     SRAMHGLVIFTDHATAPAFEQLGASPADSRTVHNFLDSCRPLMAFDREAVAKWKEIPF
                     SEARSWVQDGLGSWADFRLSLSHWASEVPFSTARLWVENGLCSWAIFHEQLMEWTDEV
                     SPALAADWVRNGLLLRRDFAHRAQQWVWAARFDDAVAWVDAGLLGWEDFQPRLAEWTD
                     RATLPLACAWVSAKACRWIDLQRHLPNWSEQATLWQARAWVEEEAISWKSFENMLSRW
                     FSEVDVDLASLWVRQGYCTWESLRHRLPSWRDTVGYSDARTWQEQGLIDWSALAHRLA
                     FWMAEASYEHACRWVEAGWCSWKDAGKRVEVWLPEASAEQAIAWLKARQCDASQAWRQ
                     CTRWRSSLTFSQASALVEAGACSWHGLAPRLGEWTELVTFLQACTWVGEELCMWTDF"
     gene            complement(3062..3997)
                     /locus_tag="PK710_15890"
     CDS             complement(3062..3997)
                     /locus_tag="PK710_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11262.1"
                     /translation="MIKNSAQIHALTFFMVTFLFSASSAVLASPLDREPEDNDRNSSQ
                     EPWNDECPTERVSMHGMGGRISVLRLSGSDADDTGRGLGLSFSGYSERYTMTHLSSRV
                     SSFVFLGGGSLGMEGGLGLDAATGFRFPAGGHQGLVLRLGLRTSLVGDDKFLESLIEI
                     PQLQLGYQWMKSNRMVEIAGRAGAVLAGRFKPGDEAARKLGKSFDVGGHGALHWDSLH
                     FDLQHTRVWLEGPAADVDFIFGRVCGDLAWFQLCVDSRIDRGGVFLPGPDDATATATS
                     IYSGLSFGTNDSWWNSGRRGDRRRASLPILTPSLW"
     gene            4410..5423
                     /locus_tag="PK710_15895"
     CDS             4410..5423
                     /locus_tag="PK710_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11263.1"
                     /translation="MLDEYSSSLERLVTIGLIASGVVGIGCTHYARLDTILDELSTMS
                     PPWFSAHRDTYRHVLATSLSQDAYLCDPLPSEVFSGSGPQGWRTIRGTMPHYGVYHGP
                     MHYDVLHHDGQWRVEVRYAVELPDPDATLELPDCALSNRLHGPMTCQGIAYSRSGTLD
                     ACPDTGTFQAPASQENVKRLLEHWSENAERYYNRDAEAFAIPVHYDFSFTPMAKDQDA
                     DGPVHWRLPLDPTCGRTPYFRSMRSGWSLPIVAHEVGHMLGLLDEYEALSGIVAWYPK
                     TAFRGAERSRFGLSMKEESVVLPLHHYLVLRRYFCPEPESRNPYGHGVPQAANIPRLR
                     GLT"
     gene            complement(5372..>6608)
                     /locus_tag="PK710_15900"
     CDS             complement(5372..>6608)
                     /locus_tag="PK710_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOT11264.1"
                     /translation="MTSPLPIIRGANLDIPSRFQAIASLLSQHTDLLHTRIQRPEAPA
                     WCHRNGWTEYLTALSDDALASSEAGEFIDILSKTRAPSSLVSLLERVRALCEVPFIDA
                     APGPSRDPGRRVKLRKRAQIDALLAALTGFGEGVRRIVDMGAGHGHFTRTIAKHWSMR
                     AIGLENNEDRVIEARRLGIDAPVEYLPWDVFSQPFALFDSDLVVGIHACGELGDVLVQ
                     RAADSRARVLLVSCCSQKVRAKVREPLSHIGKTLNLCFPRPLLGLANLHSSEWGVETS
                     LQDTMRSRQNRYALRLLLSSRGVPLEAGAEMRGMNRRQALRDFEDLARRALLFHRLSP
                     PTSEELAAYQSRAAVEFGIIRRLSLPRSALGRVIELAIVLDRASFLRERGYRTTVAQV
                     FDSYVSPRNLGIFAACGTP"
BASE COUNT         1507 a         1932 c         1894 g         1265 t
ORIGIN      
        1 ttcagcggat tcagaccacc tcggcagatt gagttcgaag tctttccagg tcgccagccc
       61 ggcgtcgacc caccctgttg cttgctcaag agacgctcct ggcttccacg aagcgatccg
      121 gtccgcgaag gcttcccaag aacataaatt caaccgaacc cattcggtgg ccagctcccg
      181 cgttgcttca ggagtccact gggcaatccg gttnnnnnnn nnngaagtcc gtccacatgc
      241 atagctcctc ccccacccac gtgcatgcct gcaaaaacgt cacaagctcc gtccattcac
      301 ccaggcgcgg ggccaggcca tgccaagaac aggctcccgc ttcgactagc gccgaagcct
      361 gagaaaaggt caacgacgaa cgccagcgcg tgcattgacg ccaagcctga gaggcatcac
      421 attggcgggc cttaagccag gctatcgcct gttccgcgga agcttccggc agccacacct
      481 ccactcgctt tcccgcatct ttccaggagc accaaccagc ttccacccac cgacacgcat
      541 gctcgtaaga tgcctcggcc atccagaaag caagccggtg cgcgagcgca gaccagtcga
      601 tcaagccttg ttcctgccac gttcgagcat cggaatatcc aaccgtatcg cgccaggaag
      661 gcaatcgatg acgcagggat tcccaggtgc aatagccttg tcgaacccag agggaagcca
      721 agtccacatc cacctcggag aaccatcgag acaacatatt ctcgaatgat ttccaactga
      781 tagcctcctc ttccacccat gccctcgcct gccatagcgt ggcctgctcg gaccagttcg
      841 gcaggtgtcg ttgcaagtcg atccatcggc aggccttagc ggatacccac gcacaggcca
      901 aaggcaaagt ggctcgatcc gtccactcgg caagacgagg ctgaaaatcc tcccatccaa
      961 gcaatccggc gtcgacccac gcgacggcgt cgtcgaatcg agcggcccat acccattgtt
     1021 gcgcccgatg agcgaaatcc cgacgcaaga gcaaaccgtt gcgaacccag tcggcggcca
     1081 acgcgggact gacttcatcg gtccattcca tcagttgttc atgaaagatc gcccaggagc
     1141 aaaggccgtt ttcaacccag aggcgagcgg tggaaaaagg gacctcggaa gcccaatgac
     1201 tcaacgacag acgaaagtcg gcccaggaac cgaggccgtc ctgaacccag gatcttgctt
     1261 cagaaaatgg aatttccttc catttggcca cggcttcgcg gtcgaaagcc atgagcggcc
     1321 gacatgagtc gagaaaatta tggacggttc tgctatccgc gggcgatgct cccagttgtt
     1381 cgaaagcggg ggcggttgcg tgatcagtga aaatcacaag accgtgcatg gcgcggctga
     1441 tggcggtgaa aagctttctt gccaggtgcg gagaggaagg ccatgagccg gcaggggccc
     1501 acaagggcag gacgtgaaac tcgaggcctt tgatgtgctc gaggtcaaga acccagaaaa
     1561 aagggttggg ggaacgttct tcgcgggctt ccagatcgag aagcacaggg catcccgcgc
     1621 gctgggcggt ctgttccgcg atttttcgtg aagtcacgca gaccagaggc gaaggacctt
     1681 cctcggagcc tgcatgtgtg gttgccccga ggtcgaagcg ttcggccagg cgctgcaaga
     1741 caagggcgat tggctcggaa ttccgtcggt tttcatgcaa ttggaagaaa gaaaaagccg
     1801 gcgcgcgatg gccgaccagg cgtaatgccg cctccacccc ttcccaggaa aaacgggtat
     1861 cgcgaacgtc ctgcgtttca tccccgcaga aaaacgcgag cttgggacgg ttacccatga
     1921 gaatacacac gagccattcg atgggcgtga ggtcctgggc ctcatcgatg aatacggcat
     1981 cgaagggcaa attcatcagt gcggattcgg cgcgatagta gtcgtaggcc ttccaagcga
     2041 gatcgagcac gtcgggagcg ccgttcaaga gcttgcggta agcatcgtaa gcgatttgga
     2101 tttcggggct gacatgcgag ggcagttggt cgccgcgggt gggttgctcg ccttgaaaag
     2161 cggccgaacc gcgcaatcgt tggagttccc accaggccgg ctcaggattc gaaggtagtt
     2221 tcgccttttc ccaggccagt cgaaagagtc ttttgccgcc tctggttgcg ccgaagtggg
     2281 cacgaagcca accgctgagg ctggtgaact cgacctttcg cctttccgcg ccggggagac
     2341 gttcaaaaat cgagcggcac caggtggcga ggctcttcga aaaggtcatg aacaccgggt
     2401 tttcgatgga tccggccaac aagagtttga gcattcggta aagaaggacg gtggttttgc
     2461 cggtaccaac cccgcctcgt aaaaataccg gtctatcctc cgccacgcgt gtcaattccc
     2521 gttgcgcggg cgatagatgc aaaagggggt caatctggct ttccgcccaa cggtccatct
     2581 cgtattcgga gaattccacg aggggtggga ggtcccgccg cgttgcccca tcaggtgtaa
     2641 caagttgaat ttgcttttga ttatccccac cccacaagga ctcgtcccac ggttggtcat
     2701 cgacgagcgc atcgatagcg cgttgtccga ttcccattct tcggacagcc gtccgaaccg
     2761 cgtcgtcgtg gtggcctgca taccataaaa cgatatggat tccatcgaag cgatcgcttt
     2821 gcgccgaacg caaggtgaaa acgagtctat ctccgcggtt gaggcgggct tcgaaaacgg
     2881 gttggtccag ggcggctagc ttttttacgc gaacgccagc gctatgccag cgttcgcggt
     2941 cgtagaccct gagtttgcgc atgcaagcgt gaaggtctgg gcgcaacgag ggagagacat
     3001 ctcgcataaa gtgtttatgc gcaagaaact caagatgttg cgccttcgag gccatcgatt
     3061 ttcaccagag agacggtgtc aagatgggga gcgacgcccg gcgcctatcg cctcgacgcc
     3121 cggaattcca ccatgaatcg ttcgtaccga acgacaagcc gctatagatc gaagtcgcgg
     3181 ttgcggtggc gtcatcgggt cccggaagaa atacgcctcc gcggtcgata cgcgaatcga
     3241 cacagagctg aaaccaagcg agatcgccac acacgcgtcc gaaaatgaag tcgacatcgg
     3301 cagcggggcc ctcgagccat actcgcgtat gttgcaaatc gaaatgcaaa gaatcccagt
     3361 gcagggcgcc gtggccgccc acatcgaagg atttaccgag tttgcgcgcc gcctcgtcac
     3421 ccggtttgaa ccgtcccgcg agtacagcac ccgcccggcc ggcgatttca accatgcggt
     3481 tagatttcat ccactggtac ccaagttgga gttgcggtat ttcgatgagc gattccaaga
     3541 acttatcatc tcccaccagg ctggtacgca atccaaggcg aagaaccaag ccctggtgcc
     3601 ccccggcggg aaaacgaaat ccggtagccg catccaatcc caagccccct tccataccca
     3661 ggctgccacc tcccaaaaaa acaaaactcg agacccggga ggataaatgc gtcatggtgt
     3721 aacgctcgct ataccctgaa aaggagaggc cgaggccgcg gccagtatcg tcggcatcac
     3781 ttccgctcag gcgcaggacg gatatacggc cgcccatgcc gtgcatggat actcgctcgg
     3841 tggggcattc atcattccag ggctcttgcg aagaattgcg gtcattgtct tctggctctc
     3901 gatccaacgg ggaagccaat actgcagaag aggccgagaa aagaaacgtg accatgaaga
     3961 atgtaagagc atgaatttgg gcactatttt tgatcatcgg catcctcctg acggccgggt
     4021 ccggcaccat gaccggaaac accttactgt cgtttccggg caccaacaag gaagcggaat
     4081 gaccccaaac ggcaagtcat tttctccgcg ctccagcgat tcccattccc gacaaatagg
     4141 ttttccatat ttcccaccca ttcctaattg ctagttgcta gttcctagtt tcaatcaccg
     4201 cctacgctgc gcctctgctc atcacaattg gccgtcatac gtgagcgact gccaattctc
     4261 ccccaacggg gacacgctcg gatacggctg accccaaacc tgatgattca aaaagtatat
     4321 catttccaca tctccatcaa tcccgtcatc tcgtaatcat tttttatcat cgaccgccaa
     4381 agaaacgggt atccttgccc caagaaagga tgcttgacga atacagcagc agcctcgagc
     4441 ggttggtgac catcgggctc atcgcttccg gtgtggttgg catcggttgc acgcattatg
     4501 cacgcctgga taccatcctg gacgagctat cgacgatgag tcctccgtgg ttttcagcgc
     4561 atcgcgatac gtatcgccac gtgctcgcga cctcgctatc ccaggacgct tacctctgcg
     4621 accctctccc tagcgaggtt ttttcgggat ctgggccgca gggatggcga accattcgag
     4681 ggaccatgcc ccattacggt gtctatcacg gaccgatgca ctacgatgtg ttgcatcacg
     4741 acggccagtg gcgtgtggag gtgcggtacg ccgtggagtt gcccgatccc gacgcaacgc
     4801 tggaactccc cgattgtgcg ctctcaaacc ggctgcacgg acctatgact tgccagggaa
     4861 tcgcctacag tcgttcggga acgctcgatg cctgtccgga taccggaacg tttcaggcac
     4921 cggccagcca ggaaaatgtc aagcggctgc tcgagcactg gtccgaaaac gcagagcgat
     4981 actacaaccg cgatgcggaa gcgtttgcga ttcccgtcca ctacgacttt tctttcacgc
     5041 ccatggcgaa agaccaggac gccgatgggc ccgtgcattg gcgtctgcct ctggatccaa
     5101 cgtgcggaag aacaccctat ttccgatcga tgcggtcagg ctggtcgcta ccgatcgtgg
     5161 cgcatgaggt cgggcatatg ttgggattgc tggacgaata cgaagcgctg tcgggtatcg
     5221 tggcatggta tcccaaaacg gcttttcggg gggcggagcg aagccgtttc gggctgtcga
     5281 tgaaggaaga atcagtggtt ttgccactgc atcattacct ggtgctgcga cgatactttt
     5341 gccccgaacc ggaaagccgg aatccctatg gtcacggggt gccgcaggct gcaaatatcc
     5401 caagattacg cggacttaca taagaatcaa aaacttgagc cacggtcgtg cggtagcctc
     5461 gttctcgcaa aaaggaggcg cggtcgagga cgatggcaag ctctatcacg cgacccaggg
     5521 cggatcgggg gagagaaagg cgccggatga tgccgaattc gacggcagcc cgggactgat
     5581 aagccgcgag ttcctcggaa gttgggggag acaagcggtg gaacagcaag gctcggcgcg
     5641 ccaagtcttc gaaatcccga agagcctggc gacgattcat tccgcgcatt tccgcgccgg
     5701 cttccaaagg cacgccacgg gatgaaagca aaaggcgcaa tgcgtaccgg ttttggcgag
     5761 agcgcatggt gtcttgcaaa ctggtctcga ccccccattc ggaggaatgg agattggcaa
     5821 gccccagcaa agggcgggga aagcagaggt tcagcgtttt tccgatgtgg gaaagcggct
     5881 cgcggacctt ggcacgcact ttctgggagc aacaagacac gagaagcaca cgagcgcggc
     5941 tatcggcggc tcgttgcacc aacacatcgc ccaactcccc acaagcgtgg ataccgacga
     6001 caaggtccga atcgaatagc gcaaacggct gggaaaagac atcccagggc aggtattcga
     6061 caggggcgtc gatgccgagc cggcgcgctt cgatgacacg atcctcattg ttttcgagac
     6121 cgatcgcgcg catgctccag tgttttgcga tcgttcgcgt gaagtggccg tggccggccc
     6181 ccatatcgac gatgcgccgg accccctctc caaaacccgt caaggcagcc agaagcgcgt
     6241 caatctgggc gcgttttctc agtttgacac gacgaccggg gtctcgagac ggcccagggg
     6301 cagcgtcgat gaacggcact tcgcagaggg cgcgaacgcg ctccaacagc gaaaccaacg
     6361 aggaaggggc gcgggtcttg gataaaatat caataaattc accagcttca gacgatgcca
     6421 gggcatcgtc ggacagtgcc gtcagatatt ctgtccagcc gtttcgatga caccaggcgg
     6481 gagcctccgg gcgttggatt cgggtatgga gcaagtccgt atgctggctg agcagcgaag
     6541 cgatggcctg aaaacgggaa gggatatcga ggttggcacc cctgattatt ggcagtgggg
     6601 acgtcatg
//
LOCUS       DAOSOR010000319         6575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_11799_length_6575_cov_4.517025, whole genome shotgun sequence.
ACCESSION   DAOSOR010000319 DAOSOR010000000
VERSION     DAOSOR010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6575
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11799_length_6575_cov_4.517025"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            364..654
                     /locus_tag="PK710_15905"
     CDS             364..654
                     /locus_tag="PK710_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11265.1"
                     /translation="MVALLLERTIERPMGTGDKRMTARSVFEDFCSCHLNMIATGTDK
                     SVAYVAPEPTTQQREILTRLTLGHLVEPGPLASRWAELYRRLRQKSWNFVCL"
     gene            792..1787
                     /locus_tag="PK710_15910"
     CDS             792..1787
                     /locus_tag="PK710_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11266.1"
                     /translation="MTQITDEQAAKAIVAVLPNSRWVGAGLAQAYLWAVDQSKTPSEI
                     ARHLYDLNCYSLTKAEELVSALATSGFLRHIKARTKTGSAENPVTKLFPAAITEQRFL
                     EQVDALRAKRKTIDYEDDRESGHTLVDFTLTEGDLRLPINVKNAGTRFENARQLVGLD
                     PDDCIPIPVYKAYDAIEKESNLVYAVAIDYGLVDSINTYLIPLLDENEAIVWRILNEF
                     SGTRIRDAEDKFVYGMTTKHWNSIRQKFATPEFRLISARKSIRILQKQPKRTPGIGLR
                     AWGTGASAEVNVHISVAEETKSWAEVFDRIVGKSLGDIIDAVNRKKTEIVYDPEI"
     gene            1798..3015
                     /gene="dcm"
                     /locus_tag="PK710_15915"
     CDS             1798..3015
                     /gene="dcm"
                     /locus_tag="PK710_15915"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HOT11267.1"
                     /translation="MSLFSGCGGLDYGFEAAGFSVRFANDYDKFSCETLHLNHRQNVV
                     HAPIEQLSTSDVKAAIGSQPGACDMLVGGPPCQPFSKSAYWSKGDTLRLDDPRANTLE
                     QYFRFVEELQPQAFLLENVHGLNYNGKEEGFQFIIERIKDINQNTGLHYVPSWRVLNV
                     ANYGVPQLRVRFFLVASRKGIRFNFPAPTHIPLEEAESSLFGDMGTMPYVSAWEAIGT
                     IPLDPGQKLEVGGKWADLLPSIPEGENYLWHTDRKGGLPLFGWRTRYWSFLLKLSKRL
                     PSWTIQAQPGSAIGPFHWTNRKLSWQEMAAIQTFPKHFVIDAPRTEIQRQIGNAVPSL
                     MAEILAREIRSQVFGQETKGEPTLRIPRCTEIPEPELIQPVPEKYLLLKGEHEPHPGT
                     GKGRSYRAGQSIL"
     gene            3412..3615
                     /locus_tag="PK710_15920"
     CDS             3412..3615
                     /locus_tag="PK710_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11268.1"
                     /translation="MARSPALFRFALLSAVVRILCAEISGLMRQLDHGLAAGRAGQGV
                     IRCGQARRRIGASTDRAHTMHPP"
     gene            3708..4655
                     /locus_tag="PK710_15925"
     CDS             3708..4655
                     /locus_tag="PK710_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11269.1"
                     /translation="MTEPSVFTYKNGMGMPVTVTLGHERLVLEQARSTVELHLDRLKG
                     LHVLQHPGGVQELFLAYEHAPGKTRVVRTNSAPGQGDFQAVVDVLVGMRPDIDLRAMP
                     SREALKKMGVTSLVKPIMLVALPLVYIGLLLVFTAPMLIHGLDKGSQEVSVTELAAGQ
                     PLESRNLLLKGHLAAEYSMKKTIMRRGVPSSVTLRIPVVEPGWNPSMPVHAVLALDNA
                     PPNELGRLARMDAYPCVVRDVLWEGLDSGDKKFFRDEGKLTLAKDVRLCRMRYAGGLS
                     DMGVFSMVMGGGIFVLVIVMAVVLVAVRRVSRRMAGTPR"
     gene            complement(4980..5228)
                     /locus_tag="PK710_15930"
     CDS             complement(4980..5228)
                     /locus_tag="PK710_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11270.1"
                     /translation="MSLVTFDLRRLFRTHFPFHAQMLERFHSDVRADNNDLHHVYRAL
                     DVLAADKDDLETLLFLHGRDLFSRHVDVLPYDLTALVD"
     gene            5310..5975
                     /locus_tag="PK710_15935"
     CDS             5310..5975
                     /locus_tag="PK710_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11271.1"
                     /translation="MAEWKVGSKLQTTFYANPKKDSAFRFRATHADDERAPKAVLCND
                     PRVQPGKLCEVRVIAVHKPKSKDHGHFEVEYLGPVTFKLDDSVWVDPAVAPLSGDGDS
                     FPACISSLVRLGGDRQIRAAHRRLVIFISYFKLLRLSQRRQGRDHPGAPPPHSVHNHP
                     KSRRTEAGTPFAIASGHGTSFFRWGPRTTPPSQAIRASPARLFRTGSGRANLARNIRG
                     DGE"
     gene            6097..6393
                     /locus_tag="PK710_15940"
     CDS             6097..6393
                     /locus_tag="PK710_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11272.1"
                     /translation="MAFSRKLRGVCPSCEARRASQTAANLVDRVLPSVALRQWMPSVP
                     YELRVPMARSSALLSAVVRILCAEISVLIRRLGQQRGDRHGATEIVATIQFFGG"
BASE COUNT         1404 a         1885 c         1887 g         1399 t
ORIGIN      
        1 ggcagggaac tcccggaata gtctcgacta tccagttatt tactggagcc ctcaatccta
       61 atccccatgc tcacctactc gtgctcgatg ggcgcctggg gttcgacccg gtcctgttcg
      121 tggtgaagct gttggatggt cgcgcagggc agtgggtaat gcgcagcggg taaaggcggc
      181 gacgcatcct tttgtctcga gattcccttg cttctgcgga tggctgcagg ttctgttctt
      241 tgtgcgtggt cgcgttggcg accgacgtgg ccgaggttcg agttgacttg cgagaggcgt
      301 tcgaatttct tatcgctatt ttatcacaca gattcgaagg tcagagcaca tgtgtcgttg
      361 ttcatggtag cgctgttgct cgagcgcacg atcgagcgtc cgatgggcac tggggacaag
      421 aggatgacgg cacgttccgt attcgaagac ttctgctcgt gtcatctcaa catgattgcg
      481 acgggaactg acaagtcagt agcgtatgtc gccccggagc cgactacaca gcagcgagag
      541 attcttactc gattgacgct tggtcatctt gtggagcctg gtcccttggc ttctcgttgg
      601 gctgagttgt atcgacgtct ccgtcaaaaa tcttggaatt tcgtatgctt atgaactgca
      661 gtggtcaaga tgggtttagt cagatgtctt acactatcct gcaatatgtt gacatgattg
      721 tgttttcctt tggaatgaac tatggaagtt cgtaggtaga acttgctagc cacaagggat
      781 aacgagaatg catgacccaa atcactgacg aacaagcagc caaagcgatc gtcgctgtgc
      841 tgcccaactc gcgatgggtc ggtgcagggc ttgcccaggc gtatctgtgg gccgttgacc
      901 aaagcaagac accttctgaa atcgcccggc atctctacga cctgaactgc tactccctga
      961 cgaaagcaga ggaactggtt tcggccctgg cgacaagcgg ttttcttaga catatcaaag
     1021 ctcgaaccaa gaccggatct gccgagaacc ctgtcaccaa gctctttcct gccgccatca
     1081 cggaacaacg ttttcttgag caagttgacg cgctccgcgc gaaacgaaaa acgatcgact
     1141 acgaagatga tagggaatct gggcacaccc tggtcgattt cacgctgact gaaggcgatt
     1201 tgcgcctacc gatcaacgtt aagaatgcag gaacgcggtt cgagaacgca agacagcttg
     1261 tcggcttgga tcctgatgac tgtattccca tccctgtcta caaagcttac gatgcgattg
     1321 agaaagagtc caaccttgtt tacgcagtag ctattgatta tggccttgtg gattcaatca
     1381 atacctacct catcccgtta ttggacgaaa acgaagcgat tgtctggcga atcctaaacg
     1441 aattttcagg aactcggata cgggatgccg aagacaaatt cgtgtacgga atgacgacga
     1501 aacattggaa cagcatccgt cagaagtttg cgacccctga gtttcggctg atttccgcac
     1561 gcaaatcgat aagaattctc cagaagcaac ccaaacgcac gccgggcatc ggccttcgcg
     1621 catggggaac tggggcaagc gccgaagtca atgtacatat ctcggttgcc gaagaaacga
     1681 agtcatgggc ggaggtgttt gacagaatcg tcggcaaaag cctcggcgat atcattgatg
     1741 cagtcaatcg aaagaagaca gaaattgtgt acgatccgga aatctgaact tcaggtcgtg
     1801 agtctgtttt ccggctgtgg cgggctggat tacgggtttg aggcagcggg gttttccgtt
     1861 cgctttgcga atgattatga caagttctcc tgtgagacgc tgcacctcaa tcatcggcag
     1921 aacgtggttc atgcccccat cgagcaactc agtacatccg atgtcaaagc ggctatcggc
     1981 tcacagccgg gggcatgcga tatgttggtc ggtggccctc catgtcagcc gttctcgaag
     2041 tcagcctact ggagcaaagg cgacacgctc cgtctggatg atccgcgggc caacacgctc
     2101 gaacagtatt tccggtttgt ggaagaactt cagccacagg ccttcctgct ggagaacgtc
     2161 cacggtttga actacaacgg aaaggaagag ggattccaat tcatcatcga gcgtatcaag
     2221 gatatcaacc agaacactgg actgcactat gtcccttcct ggcgtgtcct caatgttgcc
     2281 aattacggtg ttcctcagct tcgggtacgc tttttcctcg ttgcgtcgcg aaaaggcatt
     2341 cgattcaact tcccagcccc aacacatatt cctttggaag aggcggaatc ctccctgttt
     2401 ggagacatgg gaaccatgcc ctacgtgtcg gcatgggaag ccatcggcac aatcccgctc
     2461 gaccctgggc agaagctgga ggttggtgga aaatgggcgg atttgcttcc gtccatccct
     2521 gaaggtgaga attacctgtg gcacaccgac cgcaagggcg ggctgccgtt gtttgggtgg
     2581 agaactcgtt attggagttt cttactgaaa ctctccaaac gactccccag ttggacaatt
     2641 caggctcagc ccggctcggc cattggcccc tttcattgga ccaacagaaa actgtcgtgg
     2701 caagagatgg cagcgattca gacttttccg aagcacttcg taatcgatgc cccaagaacg
     2761 gaaatccaaa gacagattgg caacgctgtg ccttcgttga tggctgagat tcttgcgcga
     2821 gagatacggt cacaagtatt cgggcaggag accaagggag aaccaacgtt acgaatccca
     2881 agatgcacag aaatacctga accagagctg atacaaccag tgcctgagaa gtacctgctt
     2941 ctcaaagggg aacatgagcc acaccccgga acaggaaaag gacgaagtta cagagcaggc
     3001 caatcgatcc tatgatgatt ttcgtgacgg tcggggggct ctacgctctt gcgtatctcg
     3061 acgacgtatt tccacgaggt cggaccagca ggatgcggat tctgcgattc accccagtga
     3121 tgccacctcc acgcggagta caacttcccc cagacacggc gtcatccaac gtcagatcgt
     3181 gcagacgtgg ctcgagacgt tcctcgcgat ggcgagcgaa gcacacctgc gaccgctgcc
     3241 gcggcatgtc gtcgaggagt ttgctgtggc tttactcgta cgctccgcgt gggcccgtca
     3301 tgcacagcga ggcgcgcgag tcagaccgca gcaaaacccg tggaccgagt cttgccccgt
     3361 gttccctcac gccaatgagc gctgagcata ccctacgagc tgcgtttacc gatggcgcgc
     3421 agcccagcgc tgttccgttt tgccctgctc agtgcggtgg tgcgcatcct gtgtgcggaa
     3481 atctccggtt tgatgcgaca gctcgaccac ggcctagctg ctggtcgcgc aggacagggg
     3541 gttattcgct gcgggcaagc gcggcgacgc atcggtgcgt ctaccgatcg cgctcacacc
     3601 atgcatccgc cctgattccg tcttgactgc cttaacgccg cccccgcgac gtgacccgaa
     3661 acttgtcttg acttccgaaa actgatcgat aattcttatc agctcctatg actgaaccat
     3721 ccgtcttcac gtacaaaaac ggcatgggca tgccggtcac ggtcaccctc ggccatgagc
     3781 gccttgtcct ggagcaggcg cgttcaaccg tggagctgca tctggaccgc ctgaaggggc
     3841 tccatgttct gcagcacccg ggtggtgtgc aggaattgtt tctggcctat gaacatgctc
     3901 ctggcaagac cagggtcgta cgcaccaatt ctgcgcctgg ccagggggac ttccaggccg
     3961 tggtggatgt gctcgtgggc atgcgtcccg acatcgacct gcgggctatg ccgagcaggg
     4021 aggcgctcaa gaagatgggc gttaccagtc tcgtgaagcc catcatgctg gtggccctgc
     4081 cgctcgtgta tatcgggctc ctgctcgtgt ttacggcgcc catgctgatt catgggttgg
     4141 acaagggttc ccaggaggtg agcgttaccg aactagccgc cgggcaaccc ctagaaagcc
     4201 gcaatctcct gctgaagggc cacctggcgg ccgaatacag catgaagaag accatcatgc
     4261 gccggggggt tccctcctcg gtgaccctcc gcatcccggt ggtggagccc ggctggaatc
     4321 catccatgcc tgtgcatgcc gtgctcgcct tggacaacgc tcctcccaac gaactcgggc
     4381 gtttggcaag gatggacgcc tatccgtgcg tggtccgcga cgtcctttgg gagggactgg
     4441 attccggtga caagaagttc ttccgggatg aggggaagct cacgctggca aaggacgtgc
     4501 ggctctgtcg aatgcgctac gccggcgggc tttccgacat gggggtcttc tccatggtga
     4561 tgggcggggg gatcttcgta ctcgtcatcg tgatggccgt ggtgctcgtc gcggtgcgtc
     4621 gcgtgtcgag gaggatggca gggaccccgc ggtgacggtc tccgcgccgt gccactggcc
     4681 gccacggggg gcttgaaccc ggccacggtg acggcgggga cgcccggtgc atcctgctcc
     4741 ctgctcaccg tcagtcccag cacgctgcgg cacccgcagt cgaccgtcgg agccaccgca
     4801 accacgtccg tgtcggcaag ctggggcgcg tcgatgagcc ccagcttgac ccccatagtc
     4861 cgtaactatt tgaaattcaa agatgttata cggcgacgta gatacaactc agcccgacga
     4921 gaccccccgc ggctcgatgc aagaagccag gtgcccaggc tccacgagat gaccaagcct
     4981 taatcgacaa gggcagttag gtcgtatgga aggacatcga catgccgact gaaaaggtca
     5041 cgaccatgca agaacagcaa ggtttccaag tcgtccttgt cagcagccag cacatcgagc
     5101 gctcgataga catggtgaag gtcgttgttg tcggcacgta cgtctgaatg aaaccgttcg
     5161 agcatctgcg catggaacgg aaaatgcgtc cgaaacaacc gtcggaggtc aaacgtgacc
     5221 aacgacacgc cgtgctctcc tgttcccagt tgtcagagag gattcccggt tgctcactca
     5281 cctcaaccag caagcggagc gaggtcaaaa tggcggagtg gaaggttggc agcaagcttc
     5341 agaccacgtt ctacgcgaac cccaagaaag acagcgcgtt tcggtttcgc gccacccacg
     5401 ccgacgatga gcgggcgccc aaggcggtct tgtgtaacga cccgcgggtg cagcccggca
     5461 agctgtgcga ggtgcgcgtc atcgcggtgc ataagcccaa gtccaaggac cacgggcact
     5521 tcgaggtcga gtatctgggg ccggtgacgt tcaagctcga cgacagcgtc tgggtggacc
     5581 ctgcagtcgc cccgctgtcg ggagatgggg attcgtttcc cgcttgcatc tcctcgcttg
     5641 ttcgcctcgg tggcgaccgc caaattcggg cggcgcatcg ccggttggtg atattcataa
     5701 gttatttcaa gttgttacgc ctctcacaac gtcgtcaagg gcgcgatcac cctggcgcgc
     5761 cgccacccca cagcgtacat aaccacccga aatcacgacg taccgaagct ggcacaccct
     5821 tcgccatcgc ttcgggccat ggcacgtcct ttttccgatg gggtccccgt acaaccccgc
     5881 cttcgcaagc catacgtgcg tcaccagccc gactcttccg tactggttca ggtcgtgcta
     5941 acttggctcg aaacattcgt ggcgatggcg agtgaagcat acctgcgacc gctgccacgg
     6001 cacgtcgccc aagagttcca gaagtacttg cgctgcgaga ttctggcgtg gggctttgcg
     6061 cgtgcgtgtt gctgaaactg cggggcttcg atgctcgtgg ctttctcgcg caagcttcgc
     6121 ggcgtgtgcc cgtcgtgcga agcgaggcgc gcgagtcaaa ccgcagcaaa cctggtggat
     6181 cgagtcctgc ccagcgttgc cttacgacaa tggatgccga gcgtccccta cgagctgcgt
     6241 gtaccgatgg cgcgcagctc ggctctgctc agtgcggtgg tgcgcatcct gtgcgcggag
     6301 atctccgttt tgatacgacg cctcggccag caacggggag accggcacgg agccacggaa
     6361 atcgtcgcga ctatccagtt tttcggtggg tagctcaatc tcaatcccca tgctcaccta
     6421 ctcgtgctcg atggcgtgta ccacaacgac ggcacccgag ccgtcttcac cgagagccgg
     6481 gctcccaccc agagcgagat cgcgcaggta actgcacttt acaaccagct tccaaacgaa
     6541 aatcaccgga cgtccccaca ccatcgaatc caatc
//
LOCUS       DAOSOR010000320         6518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_11921_length_6518_cov_4.105214, whole genome shotgun sequence.
ACCESSION   DAOSOR010000320 DAOSOR010000000
VERSION     DAOSOR010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11921_length_6518_cov_4.105214"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..694
                     /locus_tag="PK710_15945"
     CDS             149..694
                     /locus_tag="PK710_15945"
                     /inference="COORDINATES: protein motif:HMM:NF024306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOT11273.1"
                     /translation="MPAKTQSKSPKENVIQVLNKARADEIAAILQYMADHYALDDADY
                     GAVAANMKLIAIDEMRHAEMLAERIFELGGMPVAAPSMPAKRGQKIQEALEYAVGLEH
                     QAIADYNEFLEICRANRDSISAKLFEQIIEEEQIHLNYLDNVTEHIRELGAAYLAQIA
                     GGSAEASTPGRGFVAAQGGGA"
     gene            762..1976
                     /locus_tag="PK710_15950"
     CDS             762..1976
                     /locus_tag="PK710_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOT11274.1"
                     /translation="MTPQTHVPHSTRSAVLVGTGMTPVGKLSTRPTLLMRRALELALA
                     RAGIALRQVDGLIAIPSLADPHFMEAHYFATQTGLLPRKDVIVRTIDTGGAGPVTGLI
                     EAARMIEHQACQVVAVVAGDAVSSLPKEEFLRRADQTCRDPEGELKSPVIVHAYDRVA
                     RWQMDTYGITREQLAMCSVLMSRQAHRHPLALTRTPHALAQVLDSPRMAPVTHLLECA
                     RRADGGAAIVVASSDFLKQNQIHTRGLRILGGGECSGPLYPPPVIDEDMFSCEEACAA
                     AYQASGLGVRDIDFFGLYDCYPICLIRAIEAVGICAKGEGGAWVQAKFEQTEQDYAPP
                     SFPINTHGGLLAFGAPWEVPAMYNIIEAFDQLTHRAEGRQIPNARRALVYGNGGVFSA
                     SAVAILENMPPF"
     gene            2090..2236
                     /locus_tag="PK710_15955"
     CDS             2090..2236
                     /locus_tag="PK710_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11275.1"
                     /translation="MNSRSVHVRNSRLLRAAPPLAVAKIRSHDLWAGFSPHQFGHGPW
                     AGVA"
     gene            2238..3377
                     /locus_tag="PK710_15960"
     CDS             2238..3377
                     /locus_tag="PK710_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11276.1"
                     /translation="MRTYTIPSLLASLSLLIGACNLNDSSDDDDADGANGKNGGVNSP
                     QNPGGGGGDSGNPGHNPGGSGGGQNQARALSPTTLLFERRVAADQEHIVAIDYLTKEQ
                     WVISTLEENNVPGWTVDGMAISRDRTRIVFSSMVGASSADAATMMAAQRIWSMNIDGQ
                     DWIRLTPVFKSNGTTSKIDVRNPSFSPDGQTVVYDYGEYAGGSWYVAPFLVASDGSAT
                     PALVPTNLNCSINGYGAYNPVTGDLLLKHSVCVPGSPSGLYLYPKDGSAPIQLVDGNS
                     LELTSERPVFTTDGAGILFTAQPSPGKESSLYIYLMKDQKVVRLLEGEAGIWVVNAAV
                     APDDRHIVVCLRENGNTNLWLWDVGVDPTTFEPLTQDQISCNPVF"
     gene            complement(3386..3874)
                     /gene="greB"
                     /locus_tag="PK710_15965"
     CDS             complement(3386..3874)
                     /gene="greB"
                     /locus_tag="PK710_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HOT11277.1"
                     /translation="MGSNYITPEGEKHLRDELNRLWKVERPRVTHEVAEAAAQGDRSE
                     NAEYIYGKKKLREIDRRMQYLAHRLDKLTVVREKPSGTDRVFFGAWVRLESDDGEVHQ
                     FRIVGPDETHLHEGSISIDSPMARAILRKEVGAEVVVRRPRGDTEFVIVEVRYEPFPQ
                     DQ"
     gene            3926..4654
                     /locus_tag="PK710_15970"
     CDS             3926..4654
                     /locus_tag="PK710_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT11278.1"
                     /translation="MTPTDRCLDLVRPRASDYARCMSSFRFSLVAACLLGGLAAIALL
                     PAAAASDSAPLAPNAPVVLHVGDSFVHVGFTQTLSPKFRNAGIRYVVRAKHSLYIPTL
                     VNGVGLASLMRDHKPSLVILNIGANEMRMPQPSDHAPAIRQVSELVSREQTSCVWVTP
                     PPPTDLGETGIVAEIKKNSSPCRVYDSTTIAPQLERERFDNVHPTRQAGARWAEAFWT
                     WLQTERDPSSHGWRLKPRPPESPE"
     gene            4847..5770
                     /locus_tag="PK710_15975"
     CDS             4847..5770
                     /locus_tag="PK710_15975"
                     /inference="COORDINATES: protein motif:HMM:NF015376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HOT11279.1"
                     /translation="MRPPPTRTWVGLTALMLGALPGSSVGLASPSAPPPQELTAQTAR
                     KLEPKTAWRTRTGAWRLHEITITPPRHPFADFDDGAIYTLLRHQPQWLGPASVGSPTA
                     GALLNGKPLPASPFWHVQVERRAWGTPELIACLERSVAAVHEQFPHSPLIHVGDLSEQ
                     QGGFLRGHRSHQSGLDADIGFYYRGESAWYVTATEKNLDRERTWWLVRSLITDCSVQY
                     LFMDGLVAALLREYAENVENDQAWVASLFARGSTQPGIIRHAWGHRTHLHLRIFDDAA
                     TAVGQRVQQAQAWAKAHPSLAQKAESERKHQ"
     gene            complement(5838..>6518)
                     /locus_tag="PK710_15980"
     CDS             complement(5838..>6518)
                     /locus_tag="PK710_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT11280.1"
                     /translation="GLGPVGSSLVAIAVCALAGVGMGVLITRLRVTPFIITLGGMSML
                     RGLAKGLAGEQKIDADSKGLDRLMAPGTWAVLPAGVWIGLAIALIAGAVLVYTRFGKH
                     VFAVGSNEQTARLCGVEVSRIRIAVYGLAALLTGIAGIMEFSTLTVGDPTDSFGLELE
                     VIAAVVIGGGSLSGGEGSIVGSLLGALWMTVIKTGGTHVGLPNWVQEIATGAIIVAAV
                     AVDRWRKK"
BASE COUNT         1403 a         2162 c         1638 g         1315 t
ORIGIN      
        1 gtccaccgac gacatacctt cgagcgagcc agaaccgtga tggcaacacc acgcgccacg
       61 ttactcgaaa gccctgattt cgttgcttcg cagacgggat tggaggatgg tgccgaacca
      121 tcgtaagccg tagcgcaagg agtgtctcat gccagcgaaa acccagagca agagcccgaa
      181 agagaatgtg attcaggtat tgaacaaggc ccgcgccgat gagatcgccg cgatccttca
      241 gtatatggcg gatcattatg ccttggacga tgccgactat ggtgccgtgg ctgccaacat
      301 gaaactcatt gctatcgatg agatgcggca cgccgaaatg ctcgcggagc ggattttcga
      361 actcggaggc atgcccgtgg ccgcgccaag catgcctgcc aaacgaggcc aaaaaatcca
      421 agaagccctc gaatatgccg tcggactcga acaccaggcc atcgccgatt acaacgaatt
      481 cctcgaaatc tgccgtgcca atcgagatag catcagcgcc aaactcttcg aacaaatcat
      541 cgaagaagaa cagatccacc tcaactacct cgataacgtc accgagcata tccgcgaact
      601 cggagcggca tacctcgcgc aaatcgccgg aggctcggcc gaagcatcca ctcccggacg
      661 cggtttcgtc gctgcccaag gcggcggagc ataagtcaaa acccctatca tccccaattc
      721 atcccattgc ctctcgtgtc cctttgtgcc ctgatagccc cgtgactccg caaactcacg
      781 tgccacacag cacacgctcg gccgtcctcg taggtacggg gatgaccccc gtcggcaagc
      841 tctccacacg cccaaccctg ctgatgcgaa gagcgttgga actcgctctc gctcgcgcgg
      901 gcatcgcgct tcgtcaagtc gacggcctca tcgccattcc ctccctggcc gatccgcatt
      961 tcatggaggc ccattatttc gccacccaaa cggggctgct ccctcgaaaa gacgtgatcg
     1021 ttcgcaccat cgacaccgga ggagccggcc cggtgaccgg actcatcgaa gccgcgcgaa
     1081 tgatcgaaca ccaagcttgc caggtcgtcg ccgtcgtggc cggagacgcc gtctcttcct
     1141 tgcccaaaga agaatttctt cgacgggcgg atcagacctg ccgtgacccc gaaggagagc
     1201 tgaaaagccc cgtcatcgtc catgcctatg accgcgtcgc ccgatggcaa atggatacct
     1261 acggtatcac ccgtgaacaa ctcgcgatgt gcagcgtcct catgagccgc caagcccatc
     1321 gacatcccct tgccttgacc cgcacacccc acgccctcgc gcaagtcctc gactcgcccc
     1381 gcatggctcc tgtaacccat ctgttggaat gcgcgcgaag agccgacggt ggcgccgcca
     1441 tcgtggtggc gtcatcagat tttctaaagc aaaatcaaat acatacgcgt ggtctgcgca
     1501 tccttggggg cggagaatgc tctggccctt tgtaccctcc ccccgtcatc gacgaagata
     1561 tgttctcttg tgaagaagct tgcgccgcgg cttaccaggc ttccggactc ggtgttcgcg
     1621 acatcgactt tttcggcctt tacgactgct atcccatctg tttgatccgc gctatcgaag
     1681 ccgtgggcat ctgtgcgaag ggtgaagggg gagcttgggt acaagccaaa ttcgagcaaa
     1741 ccgagcagga ttatgctcca ccatcgtttc ccatcaacac ccatgggggc ctgctcgcct
     1801 tcggcgcacc gtgggaagtc ccggccatgt acaacatcat cgaggctttc gaccaactca
     1861 cgcacagagc ggaaggtcgc cagattccca acgcacgacg cgccctcgtc tatggcaacg
     1921 gcggtgtctt ttccgctagt gcggtcgcca ttctcgaaaa catgccccct ttctgacccc
     1981 gccttcttac ctcccctcgt gcccgtccgc gccgccgttc acacccatgg tggactgccc
     2041 attcgctcgt cacgcctggc gaccttttcc atgagcgaaa ggagtgttca tgaactcgcg
     2101 aagcgttcat gtccgaaatt cgcgtcttct tcgcgcggct cccccgctgg ccgtcgcgaa
     2161 gattcgttcc catgaccttt gggcggggtt ttcccctcat caattcggcc acggtccgtg
     2221 ggcaggagta gcttgaaatg cgaacgtata cgataccatc cctccttgct tccctttcgc
     2281 ttctgattgg cgcttgtaat ctcaacgatt catccgatga cgacgacgcg gatggcgcga
     2341 atgggaagaa cggcggtgtc aactcaccac aaaacccagg tggtggtggt ggtgattcgg
     2401 gcaatcccgg ccacaatccc ggtggctccg gaggcgggca gaaccaggcc agagccttgt
     2461 cgccaaccac cttgcttttc gaacgccgcg tggccgctga ccaggagcat atcgtcgcca
     2521 tcgattattt gaccaaagaa caatgggtca tttccaccct ggaagaaaac aacgtgccgg
     2581 gatggactgt cgatggaatg gccatctcgc gcgatcgaac acgcatcgtg ttttcttcca
     2641 tggtgggagc ctcgtccgcg gatgccgcca ccatgatggc cgcgcaacga atctggagca
     2701 tgaacatcga tgggcaagac tggatacgat tgaccccggt cttcaaaagt aacggcacga
     2761 caagcaaaat tgatgtacgc aatccttcgt tctcccccga cgggcagacc gtcgtgtatg
     2821 attatggcga atatgctgga ggatcttggt atgtcgcacc ctttctggtc gcaagcgacg
     2881 gcagcgccac ccccgcgttg gttcccacca acctgaattg ttcgatcaac ggatacgggg
     2941 cctacaaccc tgtcaccgga gacctgctgc tgaaacactc ggtatgcgtg cctggctctc
     3001 caagcggtct gtacctgtac ccgaaagatg gaagcgcacc catccaactg gtcgatggca
     3061 atagcctgga attgaccagc gaacggcccg tcttcaccac agatggcgcg ggaatcctgt
     3121 tcacggccca accgtcgccc ggcaaagaat cctccttata catctacttg atgaaagacc
     3181 agaaggtcgt caggctgctc gagggagaag ccggtatttg ggtcgtcaac gcggccgttg
     3241 cccccgatga tcgccatatc gtcgtgtgcc tgcgcgaaaa cggaaacacg aacctatggt
     3301 tgtgggacgt aggagtcgat cccaccacct tcgaaccgct gacacaagat cagataagct
     3361 gtaaccctgt gttttaaaac acaatttatt ggtcttgggg aaacggttcg tagcgcacct
     3421 cgacaatcac aaattccgta tccccccggg gccgtcgcac cacaacctcc gcgccaactt
     3481 ctttgcgcaa aatggcacga gccattggcg aatcgatact gatgcttcct tcatgcaaat
     3541 gcgtttcgtc gggacccacg attcggaact gatgcacctc gccatcatcg ctttccagac
     3601 gaacccaggc gccgaaaaat acgcgatcgg tgccagatgg tttttcccgt acgacggtga
     3661 gtttgtcgag ccgatgcgcc aggtactgca tacgacgatc gatttcgcga agctttttct
     3721 ttccgtagat atattcggcg ttttcggacc ggtctccttg tgccgcggct tcggcaacct
     3781 cgtgtgtcac acggggtcgc tcgactttcc ataagcgatt gagttcgtct cgaaggtgtt
     3841 tttccccttc cggggtaata taattcgatc ccatgggcga ggcggaaact agcagaaaac
     3901 acgcggttct gtgtgatgat cgagcatgac ccccacggac cgatgcctcg atctggttcg
     3961 cccacgagct tcggattatg ctcgctgtat gtcgtctttt cgcttctccc tcgttgcagc
     4021 ctgcttgctt ggagggcttg cggccatcgc tttgctccct gccgcggcgg catcggatag
     4081 cgcaccgctt gctcccaacg cccccgtcgt cttgcatgtc ggtgattctt tcgttcacgt
     4141 aggtttcacg caaaccctca gcccgaaatt ccgtaacgca ggcatacgtt atgtcgttcg
     4201 cgcaaaacac tcgctttata tccctaccct ggtcaacggc gtcggtctcg ccagtctcat
     4261 gcgagatcac aagccctctt tggtcattct caacatcggc gccaacgaaa tgcgaatgcc
     4321 tcagccttcc gaccatgctc cagccattcg tcaagtgagc gagctggtca gccgagaaca
     4381 aacgagttgc gtatgggtaa cccctccccc tcccaccgac cttggagaaa ccggtatcgt
     4441 agcggaaata aagaaaaatt catcaccatg ccgggtctat gattccacga ccatcgcgcc
     4501 acaactcgag cgggaacgct tcgacaacgt tcatcccacc cgtcaagcag gcgcgcgctg
     4561 ggcagaagcg ttttggactt ggctccaaac cgagcgggac ccgtcctccc atggttggag
     4621 actcaaaccc cgtccaccag aatctcccga atagagcaaa accgacccag atgccccagc
     4681 acgccatcgt tgagcccagc acccatccgt catccgtttt tttcgcgttt gccctttgtg
     4741 agcgcccgat atcccccaag gacgccaacg ggatgccgcc tttcattttt cttgctttcc
     4801 gcccgttggg gatggatttt ccgcgcaaag gagtatggtt tcccccgtgc gcccacctcc
     4861 cacacgaacc tgggttgggt tgaccgccct gatgctggga gccttgccag gctcatcggt
     4921 gggtcttgcc tctccctcgg ctcccccacc gcaggaattg acggcccaaa cagcaagaaa
     4981 actcgaacca aagacggcgt ggcggactcg gacgggcgcc tggcgtctgc atgaaatcac
     5041 gatcacccca cctcggcacc ctttcgcgga cttcgacgac ggagcgattt acacgttgct
     5101 acgccaccag ccgcaatggc tcggtcccgc ttccgtggga agccccaccg ccggagccct
     5161 gctcaacggc aaaccattgc ctgcctcacc gttttggcac gtccaggtcg aacgtcgagc
     5221 ctggggcacg cccgaactca tcgcttgcct ggaacgaagc gtcgcggccg tccacgagca
     5281 atttccccac tcgcccctca tccacgtggg agacttgagc gaacaacagg gcgggtttct
     5341 acgaggtcac cgaagccacc aatccggcct cgatgcggat attggctttt actatcgcgg
     5401 tgaaagcgcc tggtatgtca ccgccacgga aaagaacctc gaccgcgaac gcacctggtg
     5461 gctcgtccgc tctctgatca cggattgttc ggtgcaatac ctgttcatgg acggcctggt
     5521 ggcagcgcta ctgcgcgaat atgccgaaaa cgtggaaaat gaccaggcgt gggtcgcttc
     5581 cctttttgcc cgaggctcca cccaaccagg catcatccgt catgcctggg gccaccgaac
     5641 ccatcttcat ctgcgaatct tcgatgatgc cgccacggcc gtgggccagc gcgtgcagca
     5701 agcccaagcc tgggccaaag cccatcccag tctcgcccaa aaggccgaat cagaaagaaa
     5761 acaccagtga ttcgcgcggg ttcacgcccc acgaccaccg gcctatttca tttaactatt
     5821 tgattttaca gtatatttta cttctttcgc caccgatcca cagccaccgc ggccacgatg
     5881 atcgctccgg tagcaatctc ttgcacccaa ttcggcaacc ccacatgcgt gccgcccgtc
     5941 ttgatcacgg tcatccacaa cgcccccaac agactgccaa cgatcgaccc ttcccccccg
     6001 gaaagcgaac cgccgccgat caccaccgcc gcaatgactt ccaattcgag tccaaacgaa
     6061 tcggtaggat ctcccaccgt cagcgtggaa aactccatga tgcccgcgat ccccgtaaga
     6121 agcgcggcaa gcccgtacac cgcaatccga atccgtgata cctccacacc gcacaaccgc
     6181 gccgtctgct cgttgctccc caccgcaaat acatgctttc cgaagcgcgt atacaccaac
     6241 accgccccgg caatcagcgc gatcgccaat ccaatccata cccccgcggg caacacagcc
     6301 cacgtccccg gtgccatcaa ccggtccaat cccttggaat ccgcatcaat tttctgctcc
     6361 cccgccagcc ccttcgccag ccctcgcaac atactcatcc cccccagcgt gatgataaat
     6421 ggagtgaccc gaagccgtgt gatcaatacc cccatgccca caccggccaa cgcgcaaacc
     6481 gcaatcgcca ccaacgaaga ccccaccggt ccaagccc
//
LOCUS       DAOSOR010000321         6496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_11966_length_6496_cov_3.697873, whole genome shotgun sequence.
ACCESSION   DAOSOR010000321 DAOSOR010000000
VERSION     DAOSOR010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11966_length_6496_cov_3.697873"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PK710_15985"
     CDS             <1..267
                     /locus_tag="PK710_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11281.1"
                     /translation="PLARQKQLKKLLTLAGIPSLLAMLLVLLLARRLSVSATYTQFKG
                     DFGYRSIAGTALGYAVVWMNGVLATAVALTVREIRRISLATRPR"
     gene            complement(435..1193)
                     /locus_tag="PK710_15990"
     CDS             complement(435..1193)
                     /locus_tag="PK710_15990"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOT11282.1"
                     /translation="MKPFTRPIITRGYELDSSMTVPLSMLLRYFEHLRWESIQQPGLG
                     LGSLFQDGFRMVVRAQQLLVHELPGFEQSLEVSLALGYVGRASLDMHHEVRRSNDGKL
                     LAQGVVTAVYLNPSGHPQAVPDFLRSLVSTTQRTTLLAAPTQDMPSNAWVRNLVVVPS
                     DVDLYQHVNHARYLDLFHDTRWLAVAAGGYGDATDSANRCIRCVSIDYQRQAVAGDEI
                     RVATWMDDEGYVDFEMQRAKDGQVLARCRFSLYS"
     gene            complement(1263..1991)
                     /locus_tag="PK710_15995"
     CDS             complement(1263..1991)
                     /locus_tag="PK710_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11283.1"
                     /translation="MDSPDEAAMVKDIHDQDVDWSEAEPPSKASMTMIDRLRLLATRR
                     EEMWWEIGVLLDEIASMRLFPAPFEDFAQFAEAEIGLTRADAIKLRRVARTFSREISM
                     RFGADRLDLLLQYLEASPRSYCAVDILRLEVLVHTTDGKDVSIPFTEISDKDLSQAVR
                     TIDRERAMIDPSIPRDVAAERDRLAVTLAGVAPQVRVRVHRSGEGIGDYSLALAGVDP
                     FNMEAVGKVLMAEGKALVKANKKQ"
     gene            complement(2065..3012)
                     /locus_tag="PK710_16000"
     CDS             complement(2065..3012)
                     /locus_tag="PK710_16000"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT11284.1"
                     /translation="MACGFMVLCLWGVLAGCGGQAALPPKAIVLNQAGAEALEQGDLA
                     VAQARFSLALEFHPEFVEALANLGLVEMQRGNFVQAEVWLDKAVSINRHMAQPHHGLG
                     LLEEHRNRRDRAVEHYREALRIDPGFVPARANLARLYFEAERIDDAREQYLRLVQVAP
                     EDPHGWTGLADCLYRLGRDEQAMRVVQEAVALIGSEAPGLRIHFARILLQKGEMATAR
                     AQLGHVTGLSGPTGCEAWAWMALSFLMEASLEKALRCAEQALSMDRNHPLATYVLAMS
                     LAQARDPSAIPWLARARALSPGNSVITEALDRLKSDNPK"
     gene            3111..3626
                     /locus_tag="PK710_16005"
     CDS             3111..3626
                     /locus_tag="PK710_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11285.1"
                     /translation="MRLLPPLALSLVILPGLGCSAEGAVSLTGSIGNVHLGIEDAAFV
                     STLQGGFDVYLELGERASGPSNITFLTFSLVNADSGSPVLSKEHLSVVSSKSTPLTIQ
                     PGNNATIHFDIGDQSQPGANLEPMELSKEERPSLCGANRLQIIGTIQDSADGARPSTL
                     TSVGFSPTGCP"
     gene            complement(3630..3836)
                     /locus_tag="PK710_16010"
     CDS             complement(3630..3836)
                     /locus_tag="PK710_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11286.1"
                     /translation="MSRDESKDGAMRQAIGVAATLGRAVKQRIGEFAIEADEQIRDKL
                     QQAKKEFEQEISGESEEETSTPES"
     gene            3945..4472
                     /locus_tag="PK710_16015"
     CDS             3945..4472
                     /locus_tag="PK710_16015"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOT11287.1"
                     /translation="MGRAKQFPLAVVSMMALGTSSCLILDENVDEISGDAPEGDVGEV
                     LKLVNQVRQEGRNCGKEGTFGRTTPLGWNEKLARAAQRHADDMASHNFMGHTGSDGST
                     MADRISDEGYAWSALGENVAAGQKTPNEVVSSWLSSPGHCANIMNPAFEHMGAARAQG
                     GSYGVVWAQTFGTSR"
     gene            complement(4469..5806)
                     /locus_tag="PK710_16020"
     CDS             complement(4469..5806)
                     /locus_tag="PK710_16020"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT11288.1"
                     /translation="MRDRKTEPSTTSGRRREDPLIGVVLLNKYRILSTLASGGMGTVY
                     RAEQLSLRREVAIKVTDKLDEASRKRFIREASILAKIQHPHILTVYDYGPMPEHRGGL
                     FMAMELLQGQTLRDRLKSKGYLSPVELIQIMHQVARGLRAAHRQNLVHRDLKPSNLML
                     VRQDDGPPLVKILDFGLVKLLNNEDATEVTQAGSVVGSAQYVAPEMIVGAEMDHRADI
                     YAFGIIMFQALAGRAPFDEPTVVNTLRAHVNNPVPPIVSFNEKAKVPPSLESLIRSCL
                     AKSPADRPSDMDEVGRALHACEWELGSIAHRSLPPASIPAPPMPVPRAGLAPWKTATA
                     IVVSALLASLGTWTAQRKGWLGAASPTAAVLHIESQPPGASVRNGSEVMGTTPLEIPL
                     DNASLARTPLRLSVQLDGHTPVDITQGPARENQRVVVVWVPSLSSPSRPAASN"
     gene            complement(6271..>6496)
                     /locus_tag="PK710_16025"
     CDS             complement(6271..>6496)
                     /locus_tag="PK710_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11289.1"
                     /translation="EAFFVMHGGYEASLDWFWGSSGGFIADSRRCRWCGDHVAAPVDV
                     QGKSLRCSHAIFSDSLESAVDCGWSGETID"
BASE COUNT         1460 a         2030 c         1655 g         1351 t
ORIGIN      
        1 ccccttgccc ggcagaaaca actcaagaaa ctattgacgc tggccgggat tccttccttg
       61 ttggctatgc tgctcgtatt gttgttggcg cgacggctca gcgtgtcggc tacctatacg
      121 caattcaaag gagacttcgg atataggtcg attgcgggta ctgccttggg ctatgcggtg
      181 gtgtggatga atggcgtact cgctaccgcc gtggctctca cggttcgaga aatccgacgt
      241 atctcgctcg cgactcgtcc gcgctgaatt cacgttgggt ttgcctcgct catcgcggga
      301 tgatttgcga cagcgcgtat ccatcgaccg tagcagccct acgcgaaacc ggtcgtttgc
      361 cttgaatccc tttcgacgtc gatatcgcct cgacgctcga caggagttca cccacgggcg
      421 ggcccatttc gacatcagct atataacgag aaacgacagc gagccaaaac ctgtccgtcc
      481 ttggctcgtt gcatctcgaa atcaacatat ccttcatcat ccatccaggt cgccacacga
      541 atctcatccc ctgcaacggc ttggcgttga taatcgatcg agacgcatct gatacaacga
      601 ttcgcggagt ctgtcgcatc tccataccca cccgccgcta ccgcaagcca ccgcgtgtcg
      661 tggaacaaat ccagatagcg agcgtggttg acgtgttggt acaaatccac gtcgctcgga
      721 accacaacca ggtttcgaac ccaggcattc gacggcatat cttgcgtggg agccgccaat
      781 aaggtcgttc gctgtgtcgt ggagacgaga gagcgcagaa aatccggaac ggcctgcgga
      841 tgccccgaag gattcaaata cacggccgta accacccctt gggcaaggag cttaccatcg
      901 ttgctccgcc gcacctcatg gtgcatgtcc agactcgcgc gcccgacata tcccagagcc
      961 aaagagactt cgagggattg ttcaaatccc ggtaattcat ggaccagcag ttgctgcgcg
     1021 cgcaccacca tgcgaaaacc atcctgaaac agcgaaccga gtcccaatcc gggctgctga
     1081 atcgactccc atcgcaagtg ctcgaagtag cgaagcagca tcgacagggg aaccgtcatg
     1141 ctggagtcca actcgtagcc gcgggtgatg atgggtctcg tgaaaggttt catgatgtgg
     1201 gcgcactctg ggcaggaacg gtgggaaggg tcaatgccag cgacggtcgt agcacatata
     1261 acctattgtt ttttattagc ttttaccaat gccttccctt ctgccatcaa cactttgcca
     1321 accgcctcca tgttgaacgg atcgacacca gccagcgcca gcgagtagtc gccgatcccc
     1381 tcgccggaac gatgaactcg cacgcgaact tgcggcgcca cgcccgctag cgtaacggca
     1441 aggcgatctc gctctgccgc cacgtcccgg ggaatcgagg ggtcaatcat ggctctttcc
     1501 cggtcgatcg tgcgaaccgc ttgcgacagg tctttgtcgg agatttcggt aaacggaatc
     1561 gaaacgtctt tgccatcggt ggtatgcacc agcacttcga ggcgaagaat gtcgacggcg
     1621 caataggacc ggggagaggc ttcgaggtat tgcaagagca gatcgagcct gtcggcgccg
     1681 aagcgcatgg atatttcgcg ggagaaggta cgagccactc gccgaagctt gatggcgtcc
     1741 gctcgggtca atccgatttc cgcttccgcg aattgagcaa aatcctcgaa aggagcggga
     1801 aacagcctca tcgaggcaat ctcatcgagc agtaccccaa tctcccacca catctcttcg
     1861 cgccgcgtgg caagcaaacg aagtcgatcg atcatcgtca tcgaggcctt cgatggcggt
     1921 tcggcctcgg accagtctac gtcctgatcg tgaatatctt tgaccatggc tgcttcatca
     1981 ggggaatcca ccccttacca agtgtcatag cgtcaacgag taccgatgaa aacggtctcg
     2041 ccccggtaaa ccccaaccac attatcattt ggggttatct gatttcaatc ggtcgagggc
     2101 ttcggtgatc acgctatttc ccggagaaag cgcgcgagcc cgggcaagcc acgggatcgc
     2161 gctcgggtca cgcgcttgcg ccaagctcat cgccagcaca taggttgcca agggatgatt
     2221 tcggtccatc gataaagctt gctccgcaca tctcaaagct ttttccagac tcgcttccat
     2281 caaaaatgac agcgccatcc aagcccacgc ttcgcatccc gtgggccccg acaagccggt
     2341 aacgtgacca agttgcgccc gagccgtcgc catttcacct ttttgaagca aaatcctggc
     2401 gaagtgaatc ctcaagccgg gggcttcact tccgatcaac gccacggctt cttgcacgac
     2461 ccgcatcgct tgctcatcac gacccagccg ataaagacag tcagcgaggc ctgtccaacc
     2521 gtggggatct tccggcgcca cttgcaccaa tcgaagatat tgttcacggg catcatcgat
     2581 tcgttccgct tcgaaataga gccgtgcaag attcgcccgc gcgggaacga atcccggatc
     2641 gattcgcaat gcctctcgat aatgctccac cgcacggtcc ctgcgattgc gatgctcctc
     2701 caacaatcca agcccatggt gcggttgtgc catatgccga ttgatcgaaa ccgctttgtc
     2761 cagccagacc tccgcttgta cgaagttgcc acgctgcatt tccacgaggc ccaggttggc
     2821 aagagcctcg acgaattcgg gatgaaattc gagggcgagg gaaaaacgcg cttgcgccac
     2881 cgccaaatcg ccttgctcca gcgcctccgc accagcctga ttcaacacaa tggccttcgg
     2941 tggcaaagca gcctgtcccc cacacccagc caacacaccc cacaagcaca agaccatgaa
     3001 cccacacgcc acattcatgc atccgtatcg gacagcgctt ggacacgttg cgaggattcg
     3061 acgcgcggct gtctcttttc ttgaggacca acactgctat cctgctaccc atgcgcctat
     3121 tacccccgct ggctctcagt ctggtcatcc tccctggtct cggttgctcc gccgaaggtg
     3181 ccgtttccct caccggttcc atcggcaatg tccaccttgg catcgaggac gccgccttcg
     3241 tttccacctt gcaaggcgga ttcgacgtct atctcgaact tggggaacgc gcttccggcc
     3301 catcgaacat aacgtttttg acattctccc ttgtgaacgc cgattccggc tcacccgtgc
     3361 tctccaaaga acacctcagt gtcgtgagtt cgaaatcaac gccgcttact attcaacctg
     3421 gaaataacgc caccatccac ttcgatatcg gtgatcaatc ccagccaggc gcaaacctcg
     3481 aacccatgga actatccaag gaagaacgcc cttccctctg cggggcgaac cgtctgcaaa
     3541 ttattggaac tattcaagat tccgccgacg gcgccaggcc ttccaccctg acttccgtag
     3601 gcttttctcc caccggatgc ccgtaatcct caggattccg gcgtcgatgt ctcttcttcg
     3661 gattccccgg aaatctcttg ctcaaactcc tttttggcct gctggagctt gtcgcgaatc
     3721 tgttcatccg cttcgatcgc gaactcgccg atccgttgct tgaccgcgcg gccgagcgta
     3781 gccgccacgc ctatcgcctg acgcatagcc ccgtctttgg attcgtctcg tgacatgacc
     3841 gcaagcgtag ctcgtagccg caagccacgc acccatcatc cacgaaattt cttcttttca
     3901 ttcgccatct ggcagccgca tgcaacttcg aacacaatga ctccatggga cgagctaaac
     3961 aatttccgct tgccgtagtg tcgatgatgg ccctcggaac gtcatcgtgt ctgattctcg
     4021 acgaaaatgt ggatgagatt tcgggcgacg cacctgaagg cgatgtgggt gaagtcctga
     4081 aattggtcaa tcaagtccgt caagagggac gaaactgcgg gaaagaagga acgtttggtc
     4141 gaaccactcc ccttgggtgg aacgaaaagc tggcgcgcgc tgcccaacgt cacgccgatg
     4201 acatggcatc ccataatttc atgggccaca caggttcgga cggaagcacg atggcggatc
     4261 gaatttccga cgaaggctac gcctggtccg cgttaggaga aaacgtggcc gcgggtcaaa
     4321 aaacgccaaa cgaagtggtg tcaagttggc tttcgagccc agggcactgc gccaacatca
     4381 tgaatccggc gttcgaacac atgggcgccg cacgcgctca aggcggctcc tatggcgtgg
     4441 tttgggctca aaccttcggg acaagccgct agtttgacgc tgccggtcgt gaaggggagg
     4501 acaacgacgg cacccatacc accacgacgc gctgattctc tcgcgccgga ccttgcgtga
     4561 tatccaccgg agtatgacca tcgagctgta ccgacaaccg caagggcgtt cgcgctaaag
     4621 aggcattgtc caggggaatt tcgagcggtg tcgttcccat cacctccgag ccgtttcgca
     4681 ccgatgcgcc cggaggttgc gactcgatat gcagcactgc cgcagtgggg ctcgcggccc
     4741 caagccatcc tttgcgctgc gctgtccacg ttccaagact tgcgagaaga gcggatacga
     4801 cgatggctgt cgcggttttc catggtgcaa gtcccgctcg cggcacaggc atcggcggtg
     4861 cgggaatact cgctggcgga agcgaacgat gcgcaatact tcccaattcc cattcgcagg
     4921 catgaagcgc ccgccccact tcatccatat ccgaaggacg gtccgcgggt gacttggcga
     4981 gacaggaacg aatgagcgac tcgagggaag gcggtacctt cgctttctca ttgaaagata
     5041 caatcggagg tacgggattg ttgacgtgcg cccgcaacgt gttgaccacc gtaggctcat
     5101 cgaaaggcgc tcgtcccgcc aacgcttgaa acatgatgat cccaaaagca taaatatccg
     5161 ctcgatggtc catctcggca ccaacgatca tttccggcgc tacatactgc gccgacccca
     5221 ccaccgaacc ggcttgggtc acctccgtgg cgtcctcatt gttgagcaac ttgacaagcc
     5281 caaaatccag tatcttcacc aaaggaggac cgtcgtcctg acgaaccagc atcaggttcg
     5341 atggcttgag atcccgatgc acgaggttct gacgatgagc cgcacgcaat ccacgggcca
     5401 cctggtgcat gatttgtatc agctccaccg gtgataaata ccctttcgat ttcagccgat
     5461 cgcgaagggt ctgcccttgc aacaactcca tcgccatgaa caagccgccg cgatgctccg
     5521 gcatcggacc atagtcatat accgtcagaa tgtgaggatg ctggatcttg gcaagaatac
     5581 tcgcttcccg aatgaacctc ttgcgagaag cctcatcgag cttgtccgtg actttgatgg
     5641 ctacttcacg cctcagcgat agttgctccg ctcgataaac cgttcccatt cccccagacg
     5701 ctagagtgga caaaatccga tatttattga gcaacacgac accaatcagc gggtcttccc
     5761 gacgtcgacc cgaagtcgtg ctcggctctg ttttcctatc gcgcatgtcc ggctccaacc
     5821 gtaagccatt gaatcatgtc ttgcaggaag cgccaggatt gtttcgcctc caccgttcaa
     5881 acatcacttt ttaaaaaaga ctgtagaatc aatatcgtgc aagacaacgg accctatact
     5941 accccacttg ccccccggct cgcctcgatc ccctctcgcc gcggctcggc gataggataa
     6001 cgcaaataga cggctatccc caatgatttg tcggcaatca acgcatagcc aaaaggcttc
     6061 gaaaacagcc cttcgcgcct tactgtcccc cacattgcga ccatcgcccc tcgcctggac
     6121 ggcgccccaa ccgaagccaa gggcctagcg aagggcagga tcataaaata tggttattat
     6181 tcagttggtt atgccataac cgccccgcta tcggttccgc ttctaggact gcactggctc
     6241 acacaaccac cccaccttcg acgtttttta tcaatcaatc gtttcgccag accatccgca
     6301 atccactgcg gactcgaggg aatcagagaa aatggcatgg gagcaacgaa gcgatttgcc
     6361 ttggacatcc accggggcag ccacatggtc cccgcaccaa cggcaccgac gcgaatcggc
     6421 gatgaaaccg ccggacgagc cccaaaacca gtccaagctc gcctcgtacc caccatgcat
     6481 cacgaaaaac gcctcg
//
LOCUS       DAOSOR010000322         6492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_11978_length_6492_cov_4.872145, whole genome shotgun sequence.
ACCESSION   DAOSOR010000322 DAOSOR010000000
VERSION     DAOSOR010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11978_length_6492_cov_4.872145"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PK710_16030"
     CDS             <1..358
                     /locus_tag="PK710_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11290.1"
                     /translation="TITDFATSQNQPKSVVANEQFVCWISHGTKQVRCKTHSGTAVQT
                     VADYTQQAPAEMTVIKDTFYWSMPDGSIHRWKQNETSSTTIVPGGKGSASLIRAYGDF
                     VYWFDTSNKTIFRVAR"
     gene            404..922
                     /locus_tag="PK710_16035"
     CDS             404..922
                     /locus_tag="PK710_16035"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT11291.1"
                     /translation="MKTSHRPQDDVQDAAHWDAVEEATELLLQGNPHDALHLLRDIVR
                     EDPRNPYAYYYMGTAMFEVGRFDAAADAYRAALRISPTYLAARVGLSHSLRIEGQLDE
                     AIRQARRVLDQVPGDGDALFALGLALASNNDRVGAVRALEAFLQTHPELEVTLEARAM
                     LEKLGPIEQDEN"
     gene            988..1317
                     /locus_tag="PK710_16040"
     CDS             988..1317
                     /locus_tag="PK710_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11292.1"
                     /translation="MILDHRGWLIHPTPAPVTWPGLCILSLSTNVIILINDKKNSTMG
                     RFARRGRVRESTASAAITTPWKDCKCMNNFCDAPFAEGAKDVSRALMVDAFANRAFSS
                     SAVRGNA"
     gene            1310..>6492
                     /locus_tag="PK710_16045"
     CDS             1310..>6492
                     /locus_tag="PK710_16045"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOT11293.1"
                     /translation="MHKLAFHRLAIVNRGEPAIRLLHALRELRAESGTDLSAIALYTE
                     PDSRSAFVQQADERYFLGSATFVDPRDGHRKSKYLDYDALERALRATRADAAWVGWGF
                     VAEHPEFADLCAKLGIVFIGPSAQVMRRLGDKIAAKIIAENSRVPVAPWSGGPVATVE
                     EARTHAQRLGYPIMIKASAGGGGRGIRGVLDDSTLAQAFESARSEALKAFGDATVFIE
                     KRVQGARHVEVQIIADSYGTTWAVGVRDCTLQRRNQKVVEEAPSPILDAAIDAQLRAA
                     AVRLANGADYRNAGTVEFLFDPGSRQFYFMEMNTRLQVEHPVTEVTTGLDLVKLQLHL
                     ASGGRLEGEPPTTVGHAIEVRLNAEDPDNGFAPAPGHIELFRMPSGPGIRVDTGVREG
                     DDVASEFDSMIAKIIAHGRDRNEAISRLRRALFDSAVVIRSGSSNKAFLLELLEHPDV
                     AQHDYDIGWIDRTHVPRANEPRAFGHVALIHAAIDVYEEAMRHARARFLAAAARGRPS
                     VDADVGHPVELRTAGQTYSVNVRRVGSSSFRVEIDGSCTEVQLDRRGPFEAWLTCCGR
                     RHRIVAVVDGLRHLVEVDGVPHRISRDEGGVVRAPSPAVTLAIHCEVGEEVHQGDRLI
                     VLEAMKMEMGVVAPCHGRIREVCVMENVQVLPGTPLVIIEPTTDSFIDSSAQKITLPI
                     DAMTSHDTEPHEACYRLLTELRRAVQGFDITDEELQTNASQRSQLCATLPPDDETMWR
                     LENEVLRTYADVTWLFRRDADHQTGPLSVQESFRTYLRAVEGAGQGLPQGFLSGLRAA
                     LAHYDVESLDRSVELEEALLALHKSQRRAEVVAGQVFRVLERKMQHTDNIQYFATSSF
                     RAILDKLIDATLGRDLALNDLAREVRYHFFDRPFFETGRNEIHKRVERDFAVVCEKQG
                     GPAWHEAMRALIACPLPLMGYFTCALMQADDHGRDLALSIMLRRLYRIRHIDDVEVRS
                     VGERRVAIAAYHHQGRRCYAIATHTYCGELKEALAAASLVAQTLVDAREISIELLTVS
                     EDYPVDLGRAPSRIRKALDAVGVSAPVNRVVVAATHPEHWRTIHYFTFEAKDGVYVEH
                     ELHRGIHPMLAERLELWRLRNFQTRQLRARDDIYLFHAVARDNPKDERLFAFVEVRDL
                     SAVRDASGQIVQLPHLERMYTEALAGIRDFQSRRSARDRLHWNRIVLYIWPDAGLSLR
                     DLARVSRRLAPLAKNLGLEKAVVRVRLPHQDTVREAVIHISNRVGTGMHLRIDGLSDQ
                     PIRSLSAYAQKVVRLRRLGLVYPYEIIRMLTPTQGTEEADFPPGEFIEYDLIQDHLVA
                     VSRPPGRNNANVVIGVITNTTAKHPEGMKRVIILGDPSREMGALAEPECNRIIQALDL
                     AERWNVPVEWFAVSSGAKISMDVGTEGLDWVARVLRRIVEFTQAGGEINVIVTGVNVG
                     GQSYWNAEATMLMHTRGILVMTEQGSMVLTGKRALEYSGGVSADDNAGIGGAQSIMEP
                     NGQAQYFATDIAQACQILFRHYDYTYVVAGEQVPRRRDTTDPADRDISQATHPTIDGV
                     PFQTIGEVFSMTCNPGRKKPFDIRAVMRAVIDSDDEPLERWARMRNAENAVVWDTYLG
                     GIPLCLIGIESRPLPRLGFVPGDGPETWTGGTLFPLSSKKVARAINAASHNRPVVVLA
                     NLSGFDGSPESMRKWQLEYGAQIGRAVVNFEGPIVFCVISRYHGGAYVVFSKTLNDRM
                     EV"
BASE COUNT         1389 a         1792 c         1898 g         1413 t
ORIGIN      
        1 aacgatcact gacttcgcca cctctcagaa ccaacccaag tccgtggtcg ccaatgagca
       61 attcgtatgc tggatatccc acggtacgaa gcaagtgcgt tgcaaaacac attcgggaac
      121 cgctgtgcaa acggtcgcag actacacgca gcaagctcca gccgaaatga cggtcatcaa
      181 ggacacgttc tattggagca tgccggatgg ttccattcat cggtggaagc agaacgagac
      241 ttcttcgacc acgatcgttc caggaggaaa aggctctgcg agcctcattc gcgcttacgg
      301 tgatttcgtg tactggttcg atacgtccaa caaaaccatt ttccgggtgg cgcggtagca
      361 cggagcgtag cgaacccttt cggtcttggg ataggtcaag agcatgaaga cgagtcatag
      421 gccgcaagac gacgttcaag atgcggctca ttgggatgcg gtggaagagg caaccgaatt
      481 gttgcttcaa ggcaatcccc acgacgcgct tcatttgctt cgcgacatcg tccgcgaaga
      541 cccgcgcaat ccttacgcct attactacat gggcacggcc atgttcgagg ttgggcggtt
      601 cgatgcggcg gcggatgcgt accgagcggc attgcgaata tccccaacgt atctcgctgc
      661 gcgagtgggg ctttcgcact ctctgcgcat cgaaggacaa ctcgatgaag ccattcgcca
      721 ggcgcgtcga gtcctcgacc aagtacctgg cgacggtgac gcgttattcg ctctgggact
      781 tgcgcttgcc tcgaacaacg atcgcgttgg cgcggtgcga gcgctcgaag catttttaca
      841 aacccatccg gaattggaag tgacactcga agcccgagcc atgctcgaaa aactcggccc
      901 catcgagcaa gacgaaaact aatccaacgt ttcctttctc tgttcttgcc attggcgcca
      961 tgaagacgcc gtgcaaaggc gtcacccatg atcctcgatc atcgaggatg gctcattcat
     1021 cccacccctg cccctgtgac atggcccggg ctttgcatcc ttagcctttc gaccaacgtc
     1081 atcatcctca tcaacgacaa gaagaattca accatggggc gattcgcccg aagggggagg
     1141 gttcgagaaa gtacggcttc ggcagcgatt acaacacctt ggaaagattg caagtgcatg
     1201 aacaacttct gtgacgcgcc attcgcagaa ggtgcgaaag acgtttcgcg tgcgttgatg
     1261 gtggatgcct ttgccaaccg tgctttttct tcctccgctg tgagaggcaa tgcataaact
     1321 cgcgttccat cgtcttgcca tcgtcaatcg tggagagccg gccattcgtt tgcttcacgc
     1381 ccttcgcgaa cttcgggccg aatcgggaac cgacctatcc gcgattgccc tatatacgga
     1441 accggatagt cggagtgctt tcgtgcaaca ggccgacgag cgctattttc ttggatccgc
     1501 cacgttcgtc gatccacgcg atgggcaccg gaaaagtaag tatctcgatt atgatgcgct
     1561 cgagcgtgcc ttgcgggcca cccgagcgga tgcggcttgg gtagggtggg gattcgtggc
     1621 tgagcacccc gagtttgccg atctatgcgc caaactaggc attgtattca tcggcccctc
     1681 cgcgcaagtc atgcgccgct tgggggacaa aattgccgcc aaaatcatcg cggaaaactc
     1741 gcgggtgccc gtcgcgccat ggagcggagg gcccgtggcc accgtcgaag aagctcggac
     1801 tcacgcacaa cggctcggct atccgattat gatcaaggca tcggcgggag gaggcggacg
     1861 agggatccgc ggtgtactgg acgactcgac tcttgcacaa gcgtttgaaa gcgctcggtc
     1921 cgaagccctg aaagctttcg gcgacgccac ggtattcatc gaaaaacgcg tgcagggcgc
     1981 tcgtcatgtc gaagtgcaga tcatcgctga ttcatacgga accacatggg ccgttggagt
     2041 tcgtgattgc accctgcaac gtcgtaacca aaaggtcgtc gaagaagctc cgtcgcctat
     2101 attggacgct gcgatcgatg cccaactgcg cgccgctgcc gttcgtcttg ccaacggcgc
     2161 ggattaccga aatgccggaa cggtcgagtt tcttttcgat ccgggatcac gccaatttta
     2221 cttcatggaa atgaacaccc gtttgcaggt ggagcatccc gtcacggaag tcacgacagg
     2281 gcttgatctc gtgaagcttc agcttcatct ggcaagcggt ggacgactcg agggagagcc
     2341 tccgaccacc gtaggacacg cgatcgaagt acgtctcaat gcggaagatc ccgataacgg
     2401 atttgccccc gctcccggtc acatcgagct gtttcgcatg ccttcgggac caggcattcg
     2461 cgtcgatacg ggggtgcggg aaggagatga tgtggcgtcg gaattcgatt ccatgatcgc
     2521 caaaatcatc gctcatgggc gcgaccgcaa cgaagccatc tcccgtttgc gtcgtgcatt
     2581 attcgatagc gccgtggtca ttcggtccgg cagcagcaac aaggcttttt tgctcgagct
     2641 gctggagcat cccgatgtgg cgcaacacga ctatgacatc ggttggatcg atcgaactca
     2701 cgttccaaga gccaatgagc ctcgcgcctt cggtcatgtc gcactcatcc acgccgccat
     2761 cgatgtgtat gaagaagcga tgcgtcatgc acgtgcccga ttccttgccg ctgccgcgcg
     2821 cggacgccct agcgtcgatg ccgatgttgg tcatccagtc gagttgagaa ctgcgggaca
     2881 gacttattcc gtcaatgtgc gacgcgtggg ttcttcttca tttcgtgttg aaatcgatgg
     2941 ttcttgtacc gaggttcagt tggatcgacg aggacctttc gaagcgtggt tgacctgttg
     3001 cggccgtcga catcggatcg tcgcggtggt ggatggattg aggcacttgg tcgaagtcga
     3061 tggagtacct catcggatat cccgcgacga aggcggtgtg gtgagggctc cgtcgcccgc
     3121 ggtgacgctt gcgattcatt gcgaagtggg cgaggaggtc catcaaggcg accgcctcat
     3181 cgttctcgaa gcgatgaaga tggagatggg tgttgtggct ccgtgccatg gccgaatccg
     3241 agaagtctgc gtgatggaaa acgtgcaagt cctgcccgga acaccgcttg tgatcatcga
     3301 gccaaccacc gattcgttca tcgattcatc tgcacaaaaa atcacgctcc ccatcgatgc
     3361 catgacctcg cacgacaccg aaccgcatga agcatgttac cgccttctga cggagcttcg
     3421 tcgcgcggtg cagggattcg atatcacgga cgaggaattg caaaccaacg cgtcgcagcg
     3481 atcgcagctt tgcgcaacgc ttccgccgga tgacgaaacc atgtggcggc tggagaacga
     3541 agttctacga acctacgcgg acgtcacgtg gttgttccgt cgagacgcgg accatcaaac
     3601 gggccccttg agcgtacaag agtcctttcg aacgtatctt cgagcggtgg agggcgcggg
     3661 acagggattg ccccagggtt ttttgtcggg gctccgcgcg gccctcgctc attacgacgt
     3721 ggaaagcctc gaccgctctg tcgaactcga agaagcattg cttgcgttgc ataagtcaca
     3781 gcgacgcgcg gaagtcgtgg cgggtcaggt cttccgcgtg ctggagcgga agatgcaaca
     3841 cactgataac attcagtatt ttgccacatc gagcttccgt gccattttgg ataaactcat
     3901 tgatgccacg ctcggccggg accttgccct gaacgatctg gcgcgggaag tgcgttatca
     3961 cttcttcgat cgtccctttt tcgaaaccgg tcgaaacgag attcacaaac gagtcgaacg
     4021 agattttgcg gtggtatgtg aaaagcaagg ggggcctgct tggcatgagg cgatgcgcgc
     4081 gttgattgct tgccccttgc ctttgatggg ttacttcaca tgcgctttga tgcaagcgga
     4141 cgatcatggc cgggatcttg ctttgagcat catgctgcgc aggttgtatc gcatccgtca
     4201 tatcgatgat gtggaagtcc gcagtgtggg ggagcgtcgt gtcgccatcg cggcgtatca
     4261 tcatcagggt cgacgatgtt atgcgattgc gacgcatacg tattgtgggg aattgaaaga
     4321 ggcgttggcg gcagcctcgc tagtcgcgca aacgctcgtg gatgctcggg aaatatctat
     4381 tgaattgctt acggtgagcg aggactatcc cgtggatttg ggtcgggcac cgagccggat
     4441 tcgaaaagcg ctcgacgccg tgggtgtttc agctcccgtg aatcgggtgg tcgttgcggc
     4501 cacgcatcca gagcattggc ggaccattca ttacttcacc ttcgaagcca aggatggcgt
     4561 ctatgtggag catgagcttc atcgaggcat ccatccgatg cttgcggaac gattggagct
     4621 atggcgtctt cgcaactttc agacacgtca gcttcgtgcg cgcgacgaca tctacttgtt
     4681 ccacgcggtg gcgcgggaca atcccaaaga tgagcgattg tttgctttcg tcgaagtgcg
     4741 tgatttgtct gcggtgcgag atgcttcggg tcaaatcgtt cagttgccgc atctcgaacg
     4801 aatgtatacg gaggctcttg cgggaatacg cgattttcaa tcccgacgct cggctcggga
     4861 caggctgcat tggaaccgaa tcgttttgta tatctggccc gatgcgggtc tcagtctcag
     4921 ggatttggca cgggtgtcgc gacgcctcgc gccccttgcc aaaaacctcg gccttgaaaa
     4981 agcagtcgtt cgagttcgtc ttccccatca agacaccgtt cgcgaggccg tcattcatat
     5041 ttccaatcgc gtcggtacgg gaatgcatct gcggatcgat gggctatccg atcaacccat
     5101 tcgttcgttg agcgcgtatg cgcaaaaagt cgttcggctg cgtcgtcttg gtctcgtata
     5161 tccctatgag atcattcgca tgctcacgcc cacgcaaggc acggaagagg cggactttcc
     5221 gcccggcgag ttcatcgaat acgacctgat acaagatcat ttggtggccg taagtcgccc
     5281 gccaggacgg aacaatgcca atgtggtgat cggcgtcatc accaacacca cggccaaaca
     5341 tcccgaagga atgaaacgcg tcatcatttt gggggatcct agccgtgaga tgggcgcatt
     5401 ggcggaaccg gagtgcaacc gtatcatcca ggcgctcgat ctcgcggagc gatggaacgt
     5461 gcctgtcgaa tggttcgcgg tttcttccgg ggccaagatc tccatggacg tggggaccga
     5521 aggactcgat tgggtagccc gcgtgctccg aagaatcgtg gaatttacgc aagcaggagg
     5581 ggaaatcaac gtcatcgtga caggcgtcaa cgtcggcggg caatcgtatt ggaatgccga
     5641 ggccaccatg ctcatgcata cgcgcggtat cctcgtcatg acagagcaag ggtccatggt
     5701 gcttacgggc aagcgagcct tggaatattc cgggggtgta tccgctgatg acaacgccgg
     5761 gatcggaggc gctcaaagca tcatggagcc caatgggcaa gcccagtact tcgcaaccga
     5821 tatcgcccaa gcctgccaaa tcctgttccg ccactatgac tacacctacg tcgtagcggg
     5881 agaacaagtg cctcgtcgac gagataccac ggatccggcg gatcgagata tcagccaagc
     5941 cacacatccc accatcgatg gggtgccatt tcagacgatc ggcgaagtgt tctcgatgac
     6001 atgcaatccg ggaaggaaaa agccgttcga catccgcgcc gtgatgcgcg cggtcatcga
     6061 cagcgatgat gagcccttgg agcgctgggc acgcatgcgc aatgcggaaa atgctgtcgt
     6121 atgggatacc tatctcgggg gcattcccct gtgcctgatc gggatagaat cccgtccatt
     6181 gcctcgtctc ggatttgttc ccggtgatgg gccggagaca tggacgggcg ggacgctttt
     6241 cccgctgtcc tcgaagaaag tcgcacgggc catcaacgcc gcgagccata accgcccggt
     6301 ggtcgtcctt gccaacttat cgggattcga cggctcgccg gaatcgatgc gtaaatggca
     6361 actcgaatat ggcgcacaga taggacgtgc cgtggtcaac ttcgagggcc ccatcgtttt
     6421 ttgcgtgatc tcgagatacc acggaggcgc ttatgtcgtg ttttcaaaaa cactcaacga
     6481 tcggatggaa gt
//
LOCUS       DAOSOR010000323         6491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_11979_length_6491_cov_3.916097, whole genome shotgun sequence.
ACCESSION   DAOSOR010000323 DAOSOR010000000
VERSION     DAOSOR010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6491
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11979_length_6491_cov_3.916097"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..651)
                     /locus_tag="PK710_16050"
     CDS             complement(145..651)
                     /locus_tag="PK710_16050"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOT11294.1"
                     /translation="MSIFGGRTEEPASYDAARQLREAVRSEMQEADEETVRIVTAVAG
                     LLATVAYADRVFAPEEVEHIRYSIGRIHGMTPQATTAICSVLRERLAELSTLHSHTFA
                     RELKELTEPHVRVEILDVLVDLAAADGEISLPEVNQLRQITSSLGLSQQDYNASQQRH
                     RDKLTSLL"
     gene            complement(704..1141)
                     /locus_tag="PK710_16055"
     CDS             complement(704..1141)
                     /locus_tag="PK710_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11295.1"
                     /translation="MLSWREVLVAVLIMAGCSGESDPRPDRGSGGSKAEPPQWLDDGA
                     PTETHSKLIPSCYDLCAWEVKAAKDCPDNALEQCKASFLAMAAGVSGPCANTLDYLCK
                     CYWNHGDPDQVSCTQGGVQSGSPTVPAPQECSDFVDGWAKCNP"
     gene            1183..1839
                     /locus_tag="PK710_16060"
     CDS             1183..1839
                     /locus_tag="PK710_16060"
                     /inference="COORDINATES: protein motif:HMM:NF021788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOT11296.1"
                     /translation="MPLFVDPVHEALIVRHTALSSPRLCPDILLHLITPAQPLWRAGQ
                     AELDALGLADPFWAFCWPGGQALAYYISRYPESVRGKRVLAFGAGCGVEAIAAARCGA
                     EVLASDTDPVAATAILLNARRNNVSLRITTDNLLGNLRSYWDIVLVGDVTYERELAKQ
                     VRAWVTQLAHHGSRVLVADPQRGFFDREGWITVAAYTPPADDDEDGTKTVMTPVLTLG
                     "
     gene            1937..2122
                     /locus_tag="PK710_16065"
     CDS             1937..2122
                     /locus_tag="PK710_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11297.1"
                     /translation="MTRIEPYPSPWEKRPNGGAIHLEAPYPIGADMSLELVVVGNVPY
                     WFLHVCGVPFELDAAKA"
     gene            complement(2127..2735)
                     /locus_tag="PK710_16070"
     CDS             complement(2127..2735)
                     /locus_tag="PK710_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11298.1"
                     /translation="MDLLDAVESGRFLGREFLLFLWFESEVLEGQFEMPDGERFDLWL
                     ENQLTLESETAEQEVTRMRGAAPSTTSEAHEALRRGKLPVQARIRIDRGQQAFSAVVS
                     ANSLSLSSATIPQLIKEEEEERFYERMYLVEELEKMIDALYEQFLSIRLSPLWETKML
                     PMIRRWVQNPTQADAKKLRTIRNEATPLGRGKKAGWILDPGE"
     gene            complement(2738..3451)
                     /gene="rdgC"
                     /locus_tag="PK710_16075"
     CDS             complement(2738..3451)
                     /gene="rdgC"
                     /locus_tag="PK710_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HOT11299.1"
                     /translation="MWYQRFSFHDCEQETSLGAIKGSLSYTRFYVWGELEGAFHDRFM
                     RSIKNRVFEPLVAQEPEEEHSGWVSIADPFDLELTRDKVFLNNYLNLAFRIDRWRLPK
                     PVFDAQFAEAERQLLERKNRERLTRKEKEELRLFVAKRLRKQVIPAMKIIDLSWDLEA
                     GLVRFWSQSPKMHERLMEIFEKTFKLKLVTESPYANAIRLGITESQAMSLLQLDLSRF
                     HGPDDGELPMASSFDPEVE"
     gene            complement(3567..4994)
                     /locus_tag="PK710_16080"
     CDS             complement(3567..4994)
                     /locus_tag="PK710_16080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11300.1"
                     /translation="MTRWALRAAKFLLQGAIMLNFSKRLPHLLPGIFLLFAGVHCSSE
                     GPTEITYDPGKDPVTVPSGGSGGEGVETGGSGGNATGGAGGSSAGAAGSAAQAGGGGD
                     SGSGGSEPPDAAPPDANFQYDAPVTDTSLNQDTACAASRVEVTLTPLDMYIMLDRSGS
                     MVEPGYSWSPKPTGGITITGGDCNYDPAVPPLNSRWCYSVYALSGYFKSAEASGNRAA
                     LQVFPVGTMNTCPPPDFLATPLVGYQTLTGGAQTLINALNNADPLGSITPTKAALHGI
                     AGFTSANKTSGRITIGILITDGIPNSCAPDDGATLGSIVDAHLKATGIRTYVISMTGV
                     SPAGYQVLEAIASKGGAPSHTQYCSGSISPCHFYDVGNGDSAVFKEVLKKIQQNAIGC
                     SYSLPTTDAGVVDLDQIAVQYTPGGTGTPIDLERVNAVGACKNNAWYYDNASPPNIVL
                     CPSTCAVVEKDDNPRVDILVGCEQS"
     gene            5220..>6491
                     /locus_tag="PK710_16085"
     CDS             5220..>6491
                     /locus_tag="PK710_16085"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOT11301.1"
                     /translation="MPKTIIEPFRIKAVEPIRLTTAEQRRELLEAARLNVFKLHADDV
                     LIDLLTDSGTGAMSANQWAAMMQGDESYAGAQSFFRLEKVIQRITGYPFFIPTHQGRA
                     AERILFSVATKPGDVIPNNTHFDTTRANLEYGQAVAVDLVIPEGLQPQNTHPFKGNID
                     LERVEDLLKRDADRIPFGMITITNNSGGGQPVSLENIRAYSELLKRYDKPLILDVCRF
                     AENAMFIKLREPGMENRTIESIVHEMFSYADGCTMSAKKDGMVNIGGFIALRDERWVE
                     AARNLLILTEGFPTYGGLAGRDLDALAVGLEEVMQEDYLRYRLRTAEYLGQRLQETGI
                     AIVQPPGGHAVYLDAKDFLPHLPKEQYPAWSLCNALYLQGGIRGVEIGSVMFGKRLDD
                     GTETYHSMELVRLAFPRRVYTQSHFDYVAEVL"
BASE COUNT         1409 a         1995 c         1686 g         1401 t
ORIGIN      
        1 ccgcatccca actccggtaa ggacccacgg ggtcgaaaga ggcatttcga cccgcgatcg
       61 ccgcctcgac cacgtcctgg caccttcgcg ttccattcct gtactccgcg gccaacacgt
      121 gcagatcggg aagataacga gctgtcataa caaggaagtg agcttatccc gatgccgctg
      181 ctgcgaagcg ttgtagtctt gctgagacag accaagcgac gatgtaattt gtcgcaattg
      241 attgacttcg ggcaagctga tttcaccgtc ggccgcggca agatcgacga gaacgtcgag
      301 gatctccaca cgaacatggg gttccgtcaa ttctttgagt tcacgcgcaa acgtgtggct
      361 gtgcaacgtg gatagctcgg cgaggcgttc gcgtaacaca ctgcagatcg cagtcgtggc
      421 ttgcggtgtc atgccatgaa tacgaccgat cgaataacga atatgctcga cctcttccgg
      481 cgcgaataca cgatcggcgt aagctaccgt agctagcagc cccgccaccg cggtgacgat
      541 gcgcaccgtt tcttcatccg cttcttgcat ctcggaacga accgcttccc gaagctggcg
      601 cgcggcatcg taagacgcag gctcttccgt tcgaccgccg aaaatactca tgatttgcta
      661 gtccattgac atgctacgtt ggcggtgcgc gatcggacac ccatcatggg ttgcacttcg
      721 cccacccatc cacaaagtca ctgcattctt gcggagcagg caccgtcgga gaaccggatt
      781 ggacgccacc ctgggtgcaa ctcacctgat caggatcccc atggttccaa tagcacttgc
      841 acagataatc gagcgtgttt gcacacggtc cgctcacccc ggcggccatg gcaaggaagc
      901 tcgctttaca ctgttcgaga gcattgtcgg gacaatcctt ggcagctttc acctcccagg
      961 cgcaaaggtc ataacaggaa ggtatgagtt tggaatgcgt ttccgtcgga gccccatcgt
     1021 cgagccattg gggtggttcc gctttggaac ctcccgaacc tcgatcggga cgcggatcgg
     1081 attccccact acaaccagcc atgatcagaa ctgctaccag gacttctcgc caactcagca
     1141 tgtgtctatg gtatccgaga cgacgcatga gagaaagaga cgatgccact gttcgtagat
     1201 ccagtccatg aagcgttgat cgtgcgtcat acggcgctgt cctcgcctcg attatgcccg
     1261 gacatcctct tgcatctcat tacaccggcg cagcctttgt ggcgggccgg acaagcggaa
     1321 ctcgatgccc tgggacttgc ggaccctttt tgggcgtttt gctggcccgg aggacaggcc
     1381 cttgcctact atatttctcg ctatcccgag tccgtacgag gaaaaagggt actcgctttc
     1441 ggagctggat gcggtgtgga agcgatcgct gccgcccgat gcggcgcgga agtcctggcc
     1501 tccgacaccg atccggtcgc cgccaccgcc attttgctca atgcccgccg aaacaacgtt
     1561 tccctgcgca tcaccaccga taatttgctg ggaaatctcc ggagctactg ggatatcgtg
     1621 ctcgtgggcg acgtcacgta tgagcgggaa ctcgcgaaac aggttcgtgc ttgggtcacg
     1681 cagctcgccc accacggaag ccgcgtactc gttgccgatc cccaacgcgg ttttttcgat
     1741 cgagaaggct ggatcaccgt ggccgcctac actccccccg ccgatgatga tgaagatggc
     1801 accaaaacag tcatgacacc cgtgcttacc ctcggttgag cgcgctgttc atcggtttca
     1861 gccatgtcca cacccacaaa gtcgaaatcg acgcttgacg tcacaacgtc cacgcgatgt
     1921 acggcatccc aggagcatga ctcgcatcga accctaccca tccccttggg aaaaacgtcc
     1981 caatggcggt gccattcatc tcgaggcccc ctaccccatc ggtgccgaca tgagcctgga
     2041 gttggtcgtg gtcggtaatg ttccttactg gttcctgcac gtttgcggtg tccccttcga
     2101 actcgatgca gcaaaggctt gagccttcac tcgcctggat cgagaatcca tcccgccttt
     2161 tttcctcgcc ccaacggcgt tgcctcgttt cgaatcgtgc gaagtttctt tgcgtccgct
     2221 tgcgtcgggt tctgaaccca acgacgaatc atcggaagca ttttcgtttc ccacagtggt
     2281 gacaaccgaa tggacaaaaa ctgttcataa agcgcatcga tcatcttctc caattcctcg
     2341 acgagataca tgcgctcgta aaatcgctct tcctcctcct ctttgatgag ttgagggatc
     2401 gtcgcactcg acagactcag tgaattggcg ctcaccaccg cactgaatgc ctgctgccct
     2461 cgatcgatac gaatgcgagc ttgcaccgga agtttgccac gtctcaatgc ctcgtgcgct
     2521 tcactggttg tgctaggcgc cgcaccacgc atccgcgtga cttcttgctc agccgtttcc
     2581 gattcgagcg tgagttggtt ctcgagccaa agatcgaaac gttccccatc gggcatctcg
     2641 aattgcccct ccaacacctc gctttcgaac caaagaaaga ggagaaactc tcggccaaga
     2701 aaacgacccg attcgacggc atccagtaga tccatgacta ttccacctcc ggatcgaacg
     2761 aggaagccat cggtagttcg ccgtcgtcag gaccgtgaaa ccgacttaga tcgagctgaa
     2821 gcagcgacat tgcctgcgat tcggtaatcc ccaaacgaat ggcattggca tacggacttt
     2881 ccgtcaccag cttgagcttg aaggtctttt cgaaaatctc catcagccgc tcgtgcatct
     2941 tcggtgattg gctccaaaac cgaacgaggc ccgcctccag gtcccacgac aagtcgatga
     3001 ttttcatggc cggaatgact tgtttgcgca accgcttggc cacgaacaac cgaagctctt
     3061 ctttttcctt gcgggtaaga cgctcccggt tcttgcgctc gagaagctga cgctccgctt
     3121 ccgcaaactg cgcatcgaat acgggtttcg ggagccgcca ccgatcgatt cgaaaggcta
     3181 agttcaagta attattcaaa aatactttgt ctcgcgtcaa ttcgagatcg aaaggatcgg
     3241 cgatcgatac ccaaccgcta tgctcctcct ccggctcctg tgccacgagc ggctcaaaca
     3301 ccctattttt gatcgagcgc ataaaccgat cgtgaaaggc tccctccagc tcaccccata
     3361 catagaagcg agtgtaacta agactccctt tgatagctcc caaactcgtc tcctgctcgc
     3421 aatcgtggaa agaaaagcgt tggtaccaca cgcgaatgat tcatccaagc gtgaggacgc
     3481 tctttccgca gcgattctca tgatgggaaa aatcagacgg aaccgaacgt gttcccttgt
     3541 ttcccggcat acgcggtcaa acgcgatcag ctttgttcgc agcccacgag gatgtccacc
     3601 cgcgggttat cgtctttttc caccaccgca caggtggaag gacacagaac gatattgggc
     3661 ggcgaagcgt tatcgtaata ccaagcgttg ttcttgcagg ctccgacggc attgacacgc
     3721 tccagatcga tgggcgttcc tgtgccccca ggcgtgtatt gcactgcaat ttgatccaaa
     3781 tccacaaccc ccgcgtccgt cgtgggaagg ctgtaggagc agccgatcgc gttctgttgg
     3841 atctttttca gcacctcttt gaacaccgcg gagtctccgt tgcccacatc gtagaaatga
     3901 caggggctga tactccccga acaatactgc gtgtgcgacg gtgctccgcc tttggaggcg
     3961 atggcttcca acacttgata tccggcaggg gacacaccgg tcatggagat cacgtaggtt
     4021 ctaattcccg tagccttgag gtgggcatcc acgatcgatc cgagcgtggc cccgtcatcg
     4081 ggcgcacaac tattggggat accatcggtg atcaagatgc cgatggtaat gcgcccggag
     4141 gttttgttgg cactggtgaa ccccgcgatg ccgtgcaagg cggccttggt cggcgtgatg
     4201 gaaccgagcg ggtcggcgtt gttgagagcg ttgataagcg tttgtgcgcc gccggtcagc
     4261 gtttgatatc cgaccaacgg ggtagccaag aagtccggag gcgggcatgt gttcatggtt
     4321 ccgacaggaa aaacctgcag ggccgcgcga tttccactgg cttccgcgga tttgaagtac
     4381 ccggagagcg cgtagacgct gtaacaccaa cgggaattca gcggtgggac ggccggatcg
     4441 taattacaat cgcctccggt gatcgtgatg ccgcccgttg gcttggggct ccatgaatac
     4501 cccggttcca ccatgctgcc ggaacggtcg agcatgatgt acatgtccag cggcgtaagc
     4561 gttacttcca cgcgcgatgc tgcacatgcc gtatcctgat tgagcgaggt atccgtcacc
     4621 ggagcatcgt attgaaaatt ggcgtccgga ggagcggcgt ccggcggctc ggaaccgccg
     4681 cttcccgaat ctcccccccc gcctgcttga gcggccgaac cggcggctcc cgccgacgat
     4741 ccgccagcgc cccctgtcgc atttccgccg cttcctcctg tttccacccc ttcaccgccc
     4801 gagccgccgc tcggtacggt aaccggatct tttcctgggt catacgtaat ttccgtgggt
     4861 ccttcgctgg agcaatgaac tcctgcaaaa agcagaaata ttcccggaag aaggtgcggt
     4921 agacgcttgg aaaaattcaa cataatggca ccctgtagca agaatttcgc cgctcgcaat
     4981 gcccatctcg tcattccctc ctttcgtaga taattcccgc gtttatctcg ggaatcggcg
     5041 ctttcgacct caccgcggct acttcgtttc accttcgcga actcttttcc gatccgcctt
     5101 cgacgagtaa gctacccgcg cttgcgtggt cgctgacgtc acaacgacgc tccttgtcgg
     5161 accgcccatg agcagaattc cgacataacc ccgagaagct ctgcaaccag gactctccca
     5221 tgcccaaaac catcatcgaa ccgtttcgta tcaaagccgt cgaacccatt cgcctgacga
     5281 ctgccgaaca acgtcgtgag ttgctcgaag cagctcggct gaacgtgttc aaactgcacg
     5341 cggacgacgt gttgatcgac ttgctcaccg attctggtac cggtgccatg agcgccaatc
     5401 aatgggccgc catgatgcaa ggcgatgaaa gctacgcggg cgcgcaaagc tttttccggc
     5461 tcgagaaagt catccaacgc atcactggct atccgttttt catcccgacc caccaaggtc
     5521 gcgccgccga acgaatccta ttttccgtcg caaccaaacc cggcgacgtg atccccaaca
     5581 acacgcactt cgacaccacg cgcgccaatt tggaatacgg ccaagcggtc gcggtcgacc
     5641 tcgtcatccc ggaagggctg caacctcaaa atacccatcc tttcaaggga aatatcgatc
     5701 tcgaacgagt cgaggacttg ctgaaacgcg acgccgatcg catcccgttc ggcatgatca
     5761 ccatcaccaa caacagcggt ggtggtcagc ccgtctccct cgaaaacatt cgagcctaca
     5821 gcgaattgct gaaacgttat gacaaaccgc tgattctcga cgtttgccgg ttcgccgaaa
     5881 acgccatgtt catcaaactg cgtgaacccg gcatggaaaa ccgcaccatc gaaagcattg
     5941 tccacgaaat gttcagctat gccgacggtt gcaccatgag tgcgaaaaaa gacggcatgg
     6001 tcaatatcgg tgggttcatt gcgttgcgcg acgaacgctg ggtggaagcc gctcgcaatt
     6061 tgctcatctt gaccgaggga ttccccacct acggcggact cgcggggcgc gacctcgatg
     6121 ctcttgccgt gggactcgaa gaagtcatgc aggaagacta tctccgttac cgtctgcgaa
     6181 ccgccgagta tctcggacaa cgactacaag aaacaggaat cgccatcgtt cagccccccg
     6241 gcggccacgc ggtgtacctg gatgccaaag acttccttcc ccatctgccc aaagagcaat
     6301 accccgcctg gagcctgtgc aacgccctgt atctccaagg aggtattcgc ggcgtcgaaa
     6361 tcggaagcgt catgttcggc aaacgcttgg atgatggcac cgaaacctat cactccatgg
     6421 aactcgtacg cctcgccttt ccacgccgcg tctataccca aagccatttc gactacgtgg
     6481 cggaagtgtt g
//
LOCUS       DAOSOR010000324         6486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_11991_length_6486_cov_4.360908, whole genome shotgun sequence.
ACCESSION   DAOSOR010000324 DAOSOR010000000
VERSION     DAOSOR010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6486
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11991_length_6486_cov_4.360908"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PK710_16090"
     CDS             complement(<1..608)
                     /locus_tag="PK710_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11302.1"
                     /translation="MAKHRHELAFLTLAVYHHPVLRVLFALLWVGSLASCGSATVEPK
                     VEHEARLLSPIPACAAVLPSPAPGVQVALRKEYWTIVFPAFDAAQGRLPADALACTGA
                     ALLQDGAFSESRVVDRLTDEQITMGGGADGIKAVWLRSHLISENGAAGALALVRTKGE
                     FAYVIAVGVHRGRIDARLSLDRLGSDIVLLVREEGCKGDKKGP"
     gene            complement(628..1236)
                     /locus_tag="PK710_16095"
     CDS             complement(628..1236)
                     /locus_tag="PK710_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11303.1"
                     /translation="MTAISLSACGGGDLAVAHQNPVFYDNSTIGGDLGSRFEVHYPPL
                     DLAAKGALPSYRGVEVLDRNVRFSRPSNWLVRAASDRAGERFIQYLSPNEYLFGIYER
                     QELPNARWADVLARYEKGLKANRAEIVHSGVPMATFNGQGRAYLIRRRIPAAKTPYIN
                     MSREYLIRAEHRIVLVQIIHPVDGLAPISAELRRAMDTLALQ"
     gene            1384..1953
                     /locus_tag="PK710_16100"
     CDS             1384..1953
                     /locus_tag="PK710_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11304.1"
                     /translation="MIPRTLLAALIGLAPCHCAAPALHTPHTPSGSEYVRDFDGKAHG
                     VSPGHVRLTPTICKGIDISQEYRILTAEAFIDYFRHQGFNISQTKARADLILVTVSGR
                     DLPEPIQFRVATLPSAGEAGRHLHLALLQHGKGRWGVHRSNLAVLGPEGSYDDIVAFA
                     ARTQLACWGVLTLAGLDDTYVIAGGYAEL"
     gene            1991..2653
                     /locus_tag="PK710_16105"
     CDS             1991..2653
                     /locus_tag="PK710_16105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11305.1"
                     /translation="MNRLALLPVAALTICASAGHAHAQSESPHRSEAWRTTSHVFVAV
                     GATSQLLMPRMYFAESETTIGWKARWHVSVLASAMTLTAASLASEYGLKPLVEGYRPG
                     CDETNQGGPGCATYGAPSTHAFASFSALGHGTSVFLVDTLKWNDGRFHGGALAGDIAL
                     PLVAAGFTILGRIGGEPNQESTGQLLAGSGLGLGFGLLSGLLYAMMQRPECGYDSGMI
                     CW"
     gene            2747..4516
                     /locus_tag="PK710_16110"
     CDS             2747..4516
                     /locus_tag="PK710_16110"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOT11306.1"
                     /translation="MSLLDKAYPNALLFSVGLLLGSGQATRDATAAQGQSEVVAPYEH
                     RLKAAQRPSSRSAWEAGARTAYESEELATLRRAESEMFGQLDDIMEELSVVSRKLDGM
                     ERRDTRESSRSDSLQPRGSQDFWQDLRLPTLSFSPNPKLEKYIRFFTESASGRKMFVT
                     WLKRSGKYRDIILSSLREHGLPPDLIAVVFIESGFQPKAVSSAGATGLWQFMPKTGRA
                     YGLSVTPRVDERRSIWSSTDAAARHLSDLFAYFQSWELALAAYNYGYERLASLSERMG
                     SRDFWTLSAVEGALPRETVLYVPKVLAVAVILNNLDRFDMANIEWEPALSGAALQVPG
                     GTRLSLVARAAGTSVETIRSLNPEFPNDLIPHRDSPYIIRIPANGLARARVMLPRLLE
                     DPRLGDDDIRTPAGFDWGRDDPDPGVFQRLSETRSQGALRGPSQAASSYFLEAQQEPP
                     PAAVASAPSVSDAPMSLMSQEPESRGSTPLSSSLSSYEPSTENQEASQRVSQAAPRSP
                     SISSGHGGKRRMKARSDREILYFVMSEQDTLQAVATRFGVDEGELRAMNRFGRRDIPK
                     AGTLLRIPVQKSEFPSGSGAGSR"
     gene            complement(4747..>6486)
                     /locus_tag="PK710_16115"
     CDS             complement(4747..>6486)
                     /locus_tag="PK710_16115"
                     /inference="COORDINATES: protein motif:HMM:NF021456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HOT11307.1"
                     /translation="DNPTVAAVQLTVGPLRRTLAVFGDRTWGKLGAQSKPAAFERMPL
                     RWERAMGGPLSDLNPIGRGFKTGVLLPNIERTDALVSSAKDTPLPACFGPLPPEWKWR
                     SSKLGTFHSRWVEERWPYFPADFDYAFFNAAPPEQQIPFLKGDESYAMIGVRPAKAVL
                     QGALPGLQPRAFAFRTQENAFFEVKLNLDTLFFDADQGKAAIVWRGLLTVQDEEATDI
                     STVFFTTTRVGEPLSPEQARERYAATTRPEEFTPLPTLPVSLPAIALPPAPARSQIML
                     WIQSHTSLRGKDLSGADLSQADLRGIDLSDCILLHANLEGARLEGANLSGAVLAHAHA
                     SRACFDGCNLSGANLAHAALTHASFRNACLTDANLWHVEASGSCFDHVQAQGVVLAKS
                     TLHQGSFQHAHVPKGDFTDCALDHTRWHHAHLEDARFYEAKGIDCSFEGALLTHARLD
                     HAHLIGADLRNANAEESTWEHANLQGASFHGAQITRANFSHANLKMSVLSMAVAKEAF
                     FRKANLQDAHLLKTNLMQANFEGADLQNADLRGANCYQVETWKAKMTGALLDLAIVAG
                     SKLEKGIPPKNRV"
BASE COUNT         1376 a         1846 c         1931 g         1333 t
ORIGIN      
        1 ggcccctttt tgtcgccttt gcacccctct tcccgaacca gaagcacgat atcgcttccc
       61 agccggtcca acgatagccg agcatcgatt cgaccacgat gaacccccac cgcaatgaca
      121 tacgcaaatt cgcctttcgt gcgaaccaga gccagagcgc ccgccgcgcc gttctccgaa
      181 atcagatggg aacgtaacca caccgccttg atcccatcag cccccccacc catcgtgatc
      241 tgttcgtcgg tcagccgatc gacaacacga ctttccgaaa aggctccgtc ttgcaataac
      301 gctgcaccag tacaagccaa cgcatcggcg ggaagacgcc cctgggctgc atcgaaggca
      361 gggaaaacaa tcgtccagta ctctttgcgc agcgccacct gcactcctgg agctggcgac
      421 ggaagcaccg cggcacaggc tggaatcgga gagagcagac gggcttcgtg ctcgaccttt
      481 ggctccaccg tcgcactgcc gcacgacgcg agcgaaccta cccataatag tgcgaaaagc
      541 acacgcagca ccggatgatg gtatacggcc aacgtcagaa aggccaactc gtggcggtgt
      601 ttcgccatcc ccaaagcact cccctgttca ttgtaacgcg agggtatcca tggcgcgacg
      661 caactccgcg ctgatgggag ccagtccatc gaccgggtga atgatctgga cgagaacgat
      721 cctgtgttcc gctcgaatca gatactcacg gctcatatta atataggggg ttttcgcagc
      781 cgggattcga cgacgaatga gataggctcg cccctggccg ttgaaggtcg ccatgggcac
      841 gccggaatga acgatttctg cccggttggc tttcagaccc ttttcataac gcgccaacac
      901 gtcggcccac ctggcattgg ggagttcctg gcgctcataa atgccaaaca ggtattcgtt
      961 gggggataaa tactggatga aacgctcgcc ggcacggtcg ctcgcggcgc gcacgagcca
     1021 attggacggg cgagaaaagc gcacgtttcg gtcgagaacc tcgacaccac gatacgaggg
     1081 gagggctcct ttggccgcca gatccagggg aggataatga acttcaaacc tggagccgag
     1141 atccccccct atcgtggaat tatcataaaa cacaggattt tggtgggcaa cggcgagatc
     1201 cccgccgcca caggcggata ggctaatcgc agtcacaacc aagctcagga ggagccgaat
     1261 cacccaccca ttgtagcgaa cccaacctcg gagaggcaaa atccaaggta gctccagaaa
     1321 gacaaggaaa tgcgtttgct caattctcgt ccctggctcc catcatcagg taaggttgat
     1381 tccgtgatcc cccgaacgct gttggcagcc ctgattggcc tggcaccatg ccattgcgca
     1441 gcaccagccc tgcacacgcc ccacacgcct tcgggaagcg aatacgtgcg ggacttcgat
     1501 ggcaaagcgc atggcgtgag cccaggccac gtccggctga cccctaccat ttgcaaggga
     1561 atagatattt ctcaagaata tcgcattctt acagccgaag ccttcatcga ttattttcgc
     1621 caccagggat tcaacatcag ccagacgaaa gcacgcgccg acctcatcct ggtcaccgta
     1681 tccggccgtg acctgccgga acccatccag ttccgcgtcg ctaccctgcc cagcgcggga
     1741 gaagccggcc gtcatcttca tctcgctctt ctgcaacatg gcaaaggacg ctggggcgta
     1801 caccgctcca accttgccgt gctcggaccc gaaggctcct acgacgatat cgtagccttt
     1861 gccgcgcgca cccaattggc atgctggggt gtacttaccc tcgctggcct cgacgatacc
     1921 tatgtcattg ccggaggcta cgcggagttg tgaacgaacc aaccatccac gaagtggaac
     1981 cacacgtacc atgaaccgcc ttgcgcttct ccctgtcgcc gccctgacca tctgcgcctc
     2041 ggcaggtcac gcccacgcgc aatccgaatc cccccatcga agcgaggcgt ggcgtaccac
     2101 atcgcacgtt ttcgtcgccg tgggcgctac gtcccaactg ctgatgcccc ggatgtactt
     2161 cgcggaatcg gaaacgacca tcggatggaa agcacgatgg cacgtttccg tccttgcttc
     2221 cgcgatgacc ctcaccgccg cttcgctcgc gagcgaatat gggctcaaac ccctcgtcga
     2281 aggctatcgc cccggatgtg acgaaaccaa ccagggcggc cctggttgcg ccacctacgg
     2341 ggccccatcg acccacgcct tcgcctcctt ctccgccctc ggccatggca ccagcgtttt
     2401 tctcgtggat acgttgaagt ggaacgatgg acgttttcac ggtggcgcgt tggcaggcga
     2461 tatcgctttg cccctggttg ccgccggatt cacgatcctc ggacgcatcg gcggcgaacc
     2521 caaccaggaa agcacaggtc aactgctcgc cggttccggc ctgggtctcg gcttcggatt
     2581 gctcagcggt ttgctctacg ccatgatgca acgccccgaa tgcggctatg actctggaat
     2641 gatctgctgg taattcccgt cccaatacac catcgcccct gatgggctcc cttcgaaaat
     2701 ccattatttt ggcagtgtag gacaaggtat gcaatagaca tacgaaatgt cacttctgga
     2761 caaagcctac ccaaacgcgc tgctgtttag cgtgggtttg ttgctcgggt ccggccaggc
     2821 aacgcgagac gccacggccg cccagggcca aagcgaagtc gtggctccgt atgagcaccg
     2881 gctcaaagcc gcgcaaaggc cgtcgtcacg atccgcgtgg gaggcagggg cgcgcacggc
     2941 ctacgaatcc gaggaattgg cgacactgcg ccgcgccgaa tcggagatgt tcggccagct
     3001 cgacgacatc atggaggagt tgtcggtggt atcgcgcaaa ctcgatggga tggagcgccg
     3061 agacacgcgt gaatcgagcc gcagcgattc gttgcaacca cgtggatccc aggatttttg
     3121 gcaagatctg cgattaccga cgctttcttt ttcgcccaac ccaaagcttg aaaagtacat
     3181 tcgttttttc acggagtcgg cctccgggcg aaaaatgttc gtcacctggc tcaaacgaag
     3241 cgggaaatac cgagacatca tcctgtcgtc cttgcgtgag cacggtcttc cgccggactt
     3301 gattgcggtg gtattcatcg aaagtggttt tcagcccaag gcggtgtcca gcgcgggggc
     3361 gaccgggctt tggcaattca tgcccaaaac ggggcgtgct tatggtcttt cggttacgcc
     3421 tcgtgtggat gaacggcgtt cgatctggag ttccacggac gcggcagctc ggcatctatc
     3481 ggatttattt gcgtattttc aatcctggga gctggcgttg gcggcctata actacgggta
     3541 tgagcgactc gcttcgcttt cggagcggat gggttccagg gatttttgga ccttgagcgc
     3601 ggtggaaggc gctttgccgc gggaaacggt gctgtatgtg cccaaggtgc ttgcggtggc
     3661 cgtaattctc aacaacctgg atcggttcga catggccaat atcgaatggg agccagccct
     3721 gtccggtgct gctcttcagg ttcccggagg aacgcgactg tctttggtgg ccagggcggc
     3781 tggcacaagc gtggaaacca ttcggtcatt gaatccggaa tttccgaatg atctcatccc
     3841 tcatcgtgat agcccttata ttatccgcat tccggcgaat ggtctggcaa gagccagggt
     3901 gatgctacct cgcttgctcg aggatcctcg gttgggcgac gatgacatac gcacaccggc
     3961 gggtttcgat tgggggcgcg acgatccgga tccgggagta ttccaacggc ttagtgaaac
     4021 caggagccag ggggctttgc gtggcccgag tcaggcagcg tcttcgtatt ttcttgaagc
     4081 ccagcaagag cctccccccg cagcagtcgc gtcggctccg agcgtcagcg atgcgccgat
     4141 gagtttgatg tcgcaagaac cggaatcgcg cggttcgacg ccattgtcct cgagcctatc
     4201 gagctacgag ccgtccacgg aaaaccaaga ggcaagccag agggtttcgc aagcggcgcc
     4261 ccgtagtcca tcgatttctt cgggtcatgg gggaaaaaga aggatgaaag cgcgatcgga
     4321 tcgcgaaatc ctttatttcg tgatgtcgga acaagacacg cttcaggcgg tggcgacgcg
     4381 gttcggcgtc gatgaggggg aacttcgtgc catgaatcgg tttgggcgaa gggacatacc
     4441 gaaagccgga acgttgttgc gcattccggt gcaaaaaagt gagttcccca gtggaagcgg
     4501 agcgggcagc cgatagaccc ggccaagcct ttcgatgctt tgctcgtaac cgatggaaat
     4561 taaacaaaat taactaagtc ttcgttcgac tccacgcatg tcccgccggt ggtcgtaccg
     4621 agaatcgtcg aatcagatgg cggaagaagc cgaaacgaaa tcgtcgagaa caagaatacg
     4681 gaaacgaatt gtatatggcc ttttctcgat acaggtctat ttcttggatt ccttattcaa
     4741 taacgattag acacggtttt tcggaggaat ccctttttcg agtttggagc cagccacgat
     4801 ggccaagtcg aggagggcac cggtcatttt ggctttccat gtttctactt ggtagcaatt
     4861 ggcgccccgc aggtcggcgt tttgcaggtc cgcgccttcg aagttggctt gcatgaggtt
     4921 ggttttcagc aagtgtgcgt cttgtaggtt tgcttttcgg aaaaaggctt ctttggcgac
     4981 ggccatggaa agcacgctca tctttaggtt ggcgtgggag aagttcgcgc gggtgatttg
     5041 cgccccatga aagctcgctc cttgcaaatt ggcgtgttcc cacgtggatt cttccgcgtt
     5101 ggcgttgcgc aggtcggcac cgatgaggtg ggcatggtcc aggcgggcgt gggtgagcaa
     5161 agcgccttcg aaggagcaat cgattccttt tgcctcgtag aagcgggcgt cctcgagatg
     5221 ggcatggtgc cagcgggtat ggtccagggc gcaatcggta aaatcgcctt ttggaacgtg
     5281 ggcatgttga aaggagccct ggtgcagggt cgatttggcg aggacgaccc cctgtgcctg
     5341 aacgtggtca aagcagctac cggaagcctc gacgtgccat agattggcgt cggtcagaca
     5401 ggcgttgcga aacgaggcat gggtgagcgc ggcatgagcg aggttggcac ctgacaggtt
     5461 gcaaccatcg aagcaggcgc ggctggcgtg ggcatgggcc aacacggccc ccgacaggtt
     5521 tgcgccttcc aatctggcac cttcgaggtt ggcgtgcagg aggatacaat cggaaaggtc
     5581 gatgccgcga aggtcggctt gggacaggtc ggcccccgag agatctttgc cccgaagaga
     5641 ggtatgtgat tgaatccaaa gcataatttg ggatcgtgcg ggagcagggg gcaaggcaat
     5701 ggccggtagg gacacgggca aggttggcag cggagtgaat tcttcggggc gcgtggttgc
     5761 ggcatagcgt tcgcgggctt gctcgggaga aagcggttct cccacccgcg tggtggtaaa
     5821 aaagaccgtg gaaatatcgg tggcttcctc gtcttggacc gtgagcaaac cgcgccagac
     5881 gatcgcggcc ttgccctggt cggcatcgaa aaagagggtg tccagattga gtttcacttc
     5941 gaaaaacgcg ttttcttggg ttcgaaacgc gaaggcgcga ggttgcaaac cgggcaaagc
     6001 gccttgaagt acggctttgg ccgggcgcac gccgatcatc gcataggatt catcaccttt
     6061 tagaaaaggg atttgctgct cgggaggggc cgcgttgaaa aaggcgtaat cgaagtccgc
     6121 gggaaaatag ggccatcttt cttcgaccca gcgggaatga aacgtgccga gtttgctgga
     6181 acgccatttc cattccggcg gcaatgggcc gaaacatgcg ggaaggggag tgtctttggc
     6241 cgacgaaacg agagcgtccg ttcgttcgat attgggtagt aacacacccg ttttgaagcc
     6301 tcttccgatg ggattgaggt ctgacaaggg gcctcccatg gctcgttccc agcgcaatgg
     6361 catacgttcg aaggcggcgg gcttggattg ggctcccaac ttgccccatg tccggtcacc
     6421 gaatacggca agggtacgtc gaagcggacc gacggtgagc tgcacggcgg ccacggtggg
     6481 gttgtc
//
LOCUS       DAOSOR010000325         6480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_12008_length_6480_cov_4.155953, whole genome shotgun sequence.
ACCESSION   DAOSOR010000325 DAOSOR010000000
VERSION     DAOSOR010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6480
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12008_length_6480_cov_4.155953"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..443)
                     /locus_tag="PK710_16120"
     CDS             complement(111..443)
                     /locus_tag="PK710_16120"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HOT11308.1"
                     /translation="MTIELAKKKCIPCEGGVDKLNPEQVNFYMTMVQGWRVDGDRIAK
                     RYVFKDFLGAMAFLNRVASAAEEQGHHPDFCVHYNQVDVTLWTHAIGGLSENDFILAA
                     VVDGLVED"
     gene            complement(581..2296)
                     /locus_tag="PK710_16125"
     CDS             complement(581..2296)
                     /locus_tag="PK710_16125"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOT11309.1"
                     /translation="MPDYGLIRLYREEFRGYDASKFQQDLLAGLTVAAVALPLALAFG
                     VASGATAAAGLITAILAGVVIGLLSGAPYQISGPTGAMSAVLIVLAQRYGLEGVFISG
                     LLSGVLLFLIGVLRLGRFIAFIPSPVIAGFTSGIALIIFIGQIDPFLGVQTAASDSAA
                     WKLIGYFKGGYIPDGRTVLLGGVVVLTMLVTPKKVSTRFPSSLLGLILATFINWAAGW
                     SVASIGQIPQTLWLSERLSFVSLASHDWTDFVAPALTIAALGAIESLLCGAVASNMTG
                     IRLQANQELIGQGVGNILIPFFGGVPATAAIARTSVGIKSGGHTRMVSLVHAVGLLLS
                     MFLLAPVMARIPLAALAGVLMVTAVRMNEWYAIRFLFGKRFKTGMATYALTLLATVAL
                     DLTQAILIGTLVSAAVFLNQLSSLDIHIQEIIPEKLRQRGIQAVGDCAHVRVAFITGP
                     LFFAATDKFNEAFKELNEMHALILSMRGVSNIDVSGLEAMRDLLHRLRKQGGTLMISG
                     IHENARKTLERGGFLDDVGQDNIFWSSDQAIVEAERRGCLYCQAGAKPDGDGTAGLGE
                     MNITS"
     gene            complement(2373..2918)
                     /locus_tag="PK710_16130"
     CDS             complement(2373..2918)
                     /locus_tag="PK710_16130"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT11310.1"
                     /translation="MRVGDHLVRARSVPGVMPQAYVSGAEAQRACEMAGKRLCTAWEW
                     WRACAGRRKMIYPYGTMHVAGRCNEERSLHPVMQLYGADAGPEVWYVEPMNNPAINAQ
                     SATVAMTGARSRCVTEDGLFDMVGNVHEWTSDPAGTFHGGAYSGKIAIGCQYITTAHG
                     FSYHDYSTGFRCCSEPVGSPE"
     gene            complement(2909..3199)
                     /locus_tag="PK710_16135"
     CDS             complement(2909..3199)
                     /locus_tag="PK710_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11311.1"
                     /translation="MRCAILGFASLMTMGCTAAPTLMVMDMDDDRPVFASKQPEASEP
                     LVVFRDWRQGHDPSKPCPADMALVADSYCVDRWEGSLVEITPSGERPFPPCV"
     gene            complement(3202..4278)
                     /gene="hrcA"
                     /locus_tag="PK710_16140"
     CDS             complement(3202..4278)
                     /gene="hrcA"
                     /locus_tag="PK710_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOT11312.1"
                     /translation="MAELNERSRRILHAIVTEFIESGEPVGSRTLSKKYGLDISPATI
                     RNVMADLEENGFLYQPHTSAGRLPTEKAFRLFVDSLMTVSSLSGDEQAEIHSLDELSP
                     GMALLRESSRVLSRLAGTASVIVAPALDRRRLREIHFVAPTCSDQVLLAVLLFFDGTV
                     ESRMVDVQQVPLDIELERMHNLLAEEVAGKTLRQLREKLASTLSQERIVLDHLTKWAY
                     ELGLLATDTELDPVVMIEGKSRLIAQPDFGDVQLLRDLLQTLDDRERLLMLLDRTIVG
                     RTVQVWVGKEAELGGGSLSLITAPFREGDSALGTIGVIGPTRMNYPKVIPLVQATAAA
                     MSEAHDRAVSDEPLPIRPKPKQPA"
     gene            complement(4344..5516)
                     /locus_tag="PK710_16145"
     CDS             complement(4344..5516)
                     /locus_tag="PK710_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11313.1"
                     /translation="MNASWQRWMGYGACVLMVGCAGASTSPAEPIGSPVSSSGSVSPA
                     VSSVSSESPDGASSAASATMPSEPELDRCAIAEPKLVVPSRRLISGFDIAEGRIFWLQ
                     HDVMTSEMTIHSLALDQESGEPELVHRVPMVNDPDALVVSQTGFWLGVDDVRGSVCKS
                     QVMWLGKAASRAVPVSEPACVFGPLRVQGAEAMWATGKTHSHPMQVSVAVGAPPGGIT
                     SLSRKADVFFDTVWHASDGVYYALERDEVIRRRPDGVEEQVVGPVNSTLPRQPKAIAL
                     WGNDIILASSQDSRKVELVRIARTGGTRSILGSFDVHSSVVKLFSNEGGLVMLLRVAR
                     DTDKLLLIDPRAECPIVELPVPDRRHRMQVVGNVAYVLQQSGIMACSFAARSPKSR"
     gene            complement(5717..5977)
                     /locus_tag="PK710_16150"
     CDS             complement(5717..5977)
                     /locus_tag="PK710_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11314.1"
                     /translation="MNRHIIYGIHIQNRGENALRVQQVLTEYGGFIKTRLGLHEMDGD
                     KGSTGGVLLLEMHGDENRYRVMRDKLSGIDGIEVKVMEFDHG"
     gene            complement(6276..>6480)
                     /locus_tag="PK710_16155"
     CDS             complement(6276..>6480)
                     /locus_tag="PK710_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11315.1"
                     /translation="WFSSGGRDIDSLVDPDALSSSEEIAAGSYVDVLSGEVISVGDKP
                     SAASWTLDSLRARVWVLESDPCR"
BASE COUNT         1563 a         2151 c         1518 g         1248 t
ORIGIN      
        1 gtcttccccc cggttttcaa ccccctcccg gatggcagcc ccccgccgga tggcaacccc
       61 ccccaggcat cccatggcct gtgcaagatg ccggtgccgc tccccaataa tcaatcctcg
      121 accaatccat cgaccaccgc ggcgagaatg aaatcattct ccgataaccc gccaatcgca
      181 tgtgtccaga gcgtcacgtc aacctgattg taatgcacgc aaaagtcagg atgatgcccc
      241 tgctcctcgg ccgccgacgc tacccgattc aaaaacgcca tcgctcccaa aaaatccttg
      301 aacacatagc gcttggcaat ccgatcccca tcaacccgcc acccctgcac catcgtcata
      361 taaaaattca cctgctccgg attcagtttg tccactcccc cttcacaggg aatgcacttc
      421 tttttcgcta actcgatcgt catagtgtac ctccacgata gtcgatgccg aagacacggc
      481 agcggtggcg ccgcgtcgca caatccctgt cgaatcgaca tggctccccg ctcagggcac
      541 cctagagcca ttggatggga tataaaaata taatgtaaat tcaactagtt atattcattt
      601 cgccaagacc cgcggtgcca tctccgtcgg gcttggcccc ggcctggcaa tacaagcaac
      661 cccgacgctc cgcctcgacg attgcctgat ccgaactcca gaagatattg tcttgtccga
      721 cgtcgtcgag aaaacctccc cgctccaacg tttttcgagc gttttcatgg atgcccgaga
      781 tcatgagcgt tccgccttgt tttcgaaggc gatgcaataa gtcccgcatc gcttcgagtc
      841 cggaaacgtc gatattggat acgccccgca tggaaagaat gagagcgtgc atttcattca
      901 gttccttgaa ggcttcattg aatttgtccg tagcggcgaa aaaaagcggt cccgtgatga
      961 aggccacacg gacatgggcg cagtcaccga ccgcttgtat tccccgctgc ctaagttttt
     1021 ccggaatgat ctcctgaatg tggatgtcga gactggataa ctgattgagg aaaacagccg
     1081 ccgacacgag cgttccaatc aggatcgcct gcgtcaagtc gagcgcgacc gtggccagaa
     1141 gcgtcaaagc atacgtggcc atgcctgtct tgaaacgttt tccgaaaagg aaacgaatcg
     1201 cataccactc attcattcga acggcggtga ccatcaacac tcccgcgaga gccgcaagcg
     1261 ggatgcgcgc catcaccgga gccaacagaa acatcgacaa cagcaagccc acggcatgaa
     1321 cgaggctcac cattcgcgta tgcccccccg acttgatccc cacactggtt cgagcaatgg
     1381 cagccgtggc cggaacaccc ccgaaaaaag gaatgaggat attgcccacg ccttgaccaa
     1441 tcagctcttg gttggcttgc aaacgaatcc ctgtcatgtt cgatgccacc gctccacaca
     1501 acaacgactc gatcgccccg agcgccgcaa tggtcaatgc cggcgccacg aaatccgtcc
     1561 agtcatgaga cgccaggctc acgaaggaca accgttcact gagccataag gtttgcggga
     1621 tttgtccaat gctagcaacg gaccagcccg ccgcccaatt gatgaaggta gccaaaatca
     1681 agccgaggag cgaagaaggg aagcgcgtgc tgaccttctt cggtgtcacg agcatcgtga
     1741 gaacaacgac ccctccaagc aacaccgtgc gcccatcagg aatgtatccg cccttgaagt
     1801 aaccgatcag cttccacgcc gccgaatccg aagccgccgt ttgcactccc aaaaatggat
     1861 cgatctgccc aatgaaaatg atgagcgcaa tgccggaggt gaaccccgcg atcaccggag
     1921 atgggatgaa cgcgatgaac cttccaagac gcaacacacc aatcaaaaac agcaacaccc
     1981 cggaaagcag acccgagata aaaacacctt ccaaaccata acgttgcgca agcacgatga
     2041 gcaccgcact catggctccc gtcggccccg atatctgata cggcgcgccc gacaacagcc
     2101 caatcaccac gccagccagg attgccgtga tcaatccagc agcagccgtc gctccagaag
     2161 ccacaccgaa tgccaacgcc aacggaagag ctaccgccgc taccgtcagc cccgccaata
     2221 aatcttgctg aaacttggaa gcgtcgtatc cacggaactc ttctcgataa agccgaatca
     2281 gtccgtaatc gggcatggtg gccctgtcct attcgccttt ctgccaatag gccaggggtc
     2341 agaacgctcc gataccctgg cggagctttc cctcactcag gcgacccaac cggctcactg
     2401 caacatcgaa atcccgtgct gtaatcgtga taggaaaacc cgtgggccgt cgtgatgtat
     2461 tgacacccaa tcgcgatttt ccccgaatac gctcccccat gaaacgtacc cgccggatcg
     2521 ctcgtccatt cgtgaacatt gcccaccatg tcaaataacc cgtcttccgt cacacatcga
     2581 gaacgcgccc ccgtcatcgc caccgtggcg ctttgcgcat tgatggctgg gttgttcatc
     2641 ggctccacat accaaacttc cggccccgca tccgcgccgt acaactgcat cacaggatgc
     2701 aacgaacgct cttcattgca tcgacccgcc acatgcatcg tcccatatgg atagatcatc
     2761 ttgcgacgtc ccgcgcaggc tcgccaccat tcccacgccg tacacagccg cttgcctgcc
     2821 atttcacacg ccctctgtgc ttcagctccc gatacatagg cttgcggcat cacacccggc
     2881 acggaacgag cccgcacgag atgatcacct acacgcatgg tgggaaagga cgttcccccg
     2941 atggtgtgat ctccacgagt gacccttccc atcgatcgac acaataactg tcagccacca
     3001 aggccatgtc cgcgggacat ggtttcgacg gatcatggcc ttgccgccag tctcgaaaca
     3061 ccaccaacgg ctcgctggcc tcgggttgct tggacgcaaa aacaggccga tcatcatcca
     3121 tgtccatgac catgagtgtg ggagcagccg tgcatcccat ggtcatgaga cttgcgaacc
     3181 caaggattgc acaacgcatg attacgcggg ttgtttgggc ttcggccgaa tcggaagcgg
     3241 ttcatcgctc acagccctgt catgcgcttc ggacatcgcc gcggcggttg cctgcaccag
     3301 cggaatgacc ttcgggtagt tcatgcgcgt cggtccaatc acaccgatgg ttcccaaggc
     3361 gctatcccct tcccgaaatg gtgccgtaat caggctcaaa gacccacccc caagctctgc
     3421 ttccttgccc acccatacct gcaccgtcct gcccacgatc gttcgatcca atagcatgag
     3481 aagacgctcc cggtcgtcga gggtctgaag caaatcccgc aacaactgca catcgccaaa
     3541 gtccggttgc gcaatcaatc gagacttacc ctcgatcatg accaccggat ccaactccgt
     3601 gtccgtcgcc aataacccaa gctcataggc ccacttcgtc aaatgatcga gtacgatacg
     3661 ctcttgactc aacgtcgatg cgagcttctc ccgaagctgg cgcaatgtct ttcccgccac
     3721 ttcttccgcc aacaagttat gcatccgctc gagttcgata tccaatggca cctgctgcac
     3781 atccaccatg cgcgactcca ccgtgccatc gaaaaataga agcaccgcaa gcaatacctg
     3841 atccgaacaa gtcggcgcaa cgaagtgaat ctctcgaagc cgacgacgat cgagagctgg
     3901 cgctacgatg accgaagcgg ttcccgccaa tcgcgacaat acgcggctgc tctcgcgcaa
     3961 caacgccatc ccaggggaaa gctcgtccag cgagtgaatc tccgcttgct catctcccga
     4021 aagcgaggaa accgtcatga gcgaatccac gaacaatcga aacgcttttt ccgtgggaag
     4081 cctccccgca gaggtgtgcg gctggtacaa aaaaccgttc tcctccaagt ccgccatcac
     4141 attgcgaatg gtcgccggag aaatgtcaag cccgtacttc ttgctgagcg ttcgactccc
     4201 cacaggctca ccggactcga tgaactcggt tacgatagcg tgaagaatgc gacgagatcg
     4261 ctcattcagc tcagccatga cgggagtcat cttgggccct cgtccgctgg ccgtcaattc
     4321 actgaggcga tttgccgaat gctctaccgt gatttcgggc ttcgcgccgc gaacgaacaa
     4381 gccatgattc cgctttgctg caacacgtaa gctacattgc ccacgacctg catccgatga
     4441 cgacggtccg gcactggcaa ttccacgatg ggacactcgg ccctcggatc gatgagcaat
     4501 aatttatcgg tgtcccgcgc aacccgaaga agcatcacca gccccccctc gttcgaaaat
     4561 agcttaacaa ccgatgaatg gacgtcgaag ctgccaagga tcgaacgggt tccacccgtt
     4621 cgcgctattc gtaccaactc cacctttcgc gaatcttggc tcgaagccag gatgatgtcg
     4681 ttgccccata gcgcgatcgc tttcggttgt cggggcagtg tcgaattcac cggcccaacg
     4741 acttgttctt cgaccccgtc gggcctgcgc cgaatgacct catcacgttc gagcgcgtag
     4801 tacaccccat cgctcgcatg ccaaaccgtg tcgaaaaaaa catcggcctt gcgagataac
     4861 gacgttatcc caccaggggg agcgccaacc gccaccgaaa cctgcatcgg gtgagaatgg
     4921 gtcttgcccg tagcccacat cgcttccgct ccctgcaccc gtaaaggacc aaatacacaa
     4981 gcaggttcgc tcaccggcac agcgcgagat gccgcttttc ccaaccacat cacctgggac
     5041 ttacaaaccg aacccctcac gtcatcaacc cccaaccaaa aaccagtttg gcttaccacc
     5101 aaagcatcgg gatcgttgac catcggtacc cgatggacca gttccggctc ccccgattct
     5161 tggtcgagcg ccagactgtg aatcgtcatc tccgatgtca tgacgtcatg ctgaagccaa
     5221 aaaattcgtc cctcggcaat atcaaacccg ctgatcaatc gacgcgacgg caccaccagc
     5281 ttgggctccg caatcgcaca gcgatcgagt tcgggctcgc tcggcatcgt cgccgacgcc
     5341 gcggatgatg ccccgtcagg tgactcgctg cttaccgaag atacggcagg cgaaacggac
     5401 cccgaagaag aaacaggcga cccgataggc tctgccggag acgtagaagc cccagcgcat
     5461 cccaccatca gtacacacgc gccgtatccc atccaccgct gccatgatgc attcatgact
     5521 tgatgatatt gtcacgaagc gcacaacggt agcgcccgct tggcgttcgt cggtcgattg
     5581 tgatgaaccc ccctcccaga gccttcaact acgtcaactt tttcccgtac tcccgtacgt
     5641 cgaacccgac gctcagcaac gtgatttcga tctgccgtgc atgaccgatc ggacaggaag
     5701 aaccatgtcc atcgctctag ccgtgatcga attccatcac cttgacttcg atcccatcga
     5761 tgccactgag cttgtcgcgc atcacacgat atcgattctc atccccatgc atctccagca
     5821 atagcacccc acccgtggaa cccttgtccc cgtccatctc atgcaatcca agtctcgtct
     5881 tgatgaaacc accatactcc gtcaaaacct gctggacgcg aagagcgttt tccccacggt
     5941 tctgaatgtg aatcccataa atgatatgcc tgttcatgcc tctgctcctt gcctcgggaa
     6001 tacccgcggc ccttgcttga taaccaccga agagaataaa aagaaatgtg aattaattcc
     6061 acgagttgca cggaatgagg aagggtgatt tcccccttcc gaaacgccga ttcccccctg
     6121 cctaagacac gactacgcaa taccaccgcc cgctggatgc taagccctta gccagggttt
     6181 cggcttgtag aacccacgga cgaacacgag gggaaccatc atcccgcatg gcaggcgcgc
     6241 ccatatcgcc gaaccctgcc atccaatccc gttgctcaac gacaagggtc ggactccaat
     6301 acccatacgc gcgcacgcaa agaatcaagc gtccatgaag ccgctgatgg cttgtccccc
     6361 accgaaatca cttctcccga caagacgtcg acataagacc cggccgcgat ctcctccgac
     6421 gaagagagag cgtctgggtc gacgaggctg tcgatatcgc gaccaccact ggaaaaccat
//
LOCUS       DAOSOR010000326         6456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_12084_length_6456_cov_3.990783, whole genome shotgun sequence.
ACCESSION   DAOSOR010000326 DAOSOR010000000
VERSION     DAOSOR010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6456
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12084_length_6456_cov_3.990783"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="PK710_16160"
     CDS             <1..733
                     /locus_tag="PK710_16160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOT11316.1"
                     /translation="KGELMGLLTERLVERYTVTRQEQDEIAFRSHHNALSAWTNGYFD
                     DYVVPVSIPHRKGEPLLVAKDEGPKPVTMEALGAAKPYFKPEGGTITSLNASSLNDAA
                     AAVMLTTEDRAKELGLVPLAELRAFHNVGVEREYMGEGAFKVIPPLLEKIRIGVGDVD
                     LFEINEAFAAVVAGAYHFIEGLAVDKTNLWGSGISLGHPVGCTGARQIVDMVHQLRKR
                     DKSVGVTTRCVGGGIGSGEVLIRMN"
     gene            complement(804..1451)
                     /locus_tag="PK710_16165"
     CDS             complement(804..1451)
                     /locus_tag="PK710_16165"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HOT11317.1"
                     /translation="MNDSVDDPCRAIPKVVLASTSPYRKQLLARLRIAFEAVDSGIDE
                     TRFKALALPPSELVRILAEQKAQAVGHHHPNSLIVGGDQCVELDGRVLGKAGTNQGAM
                     GQLRLLSGRTHHLFTALSLLDTRSGRCDTVVDVHEMTMRPLNPRQIERYVQIDEPLDC
                     AGSYKLESLGIALFDRVQGDDPTAIIGLPLMRLVSLLATAGYDVFARAGSDARKA"
     gene            1482..2039
                     /gene="orn"
                     /locus_tag="PK710_16170"
     CDS             1482..2039
                     /gene="orn"
                     /locus_tag="PK710_16170"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HOT11318.1"
                     /translation="MEDPTNNPLVWLDMEMTGLEPLTCVPLQMAVVITDSELEELDSH
                     EVTLWQPEEVLSRMDPFVRKMHTENGLLDRVRASDVGMLQAEREIMARVSRYCPFGAG
                     ILAGNSIHTDRAFLKAYFPTLHGYLHYRMVDVSSLKELVRRWYGRDWLFIKQLQKHTA
                     LEDVRESIAELRHYRLTCMRSPNKS"
     gene            complement(2046..2747)
                     /locus_tag="PK710_16175"
     CDS             complement(2046..2747)
                     /locus_tag="PK710_16175"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOT11319.1"
                     /translation="MPCCFPPALNAVLFDFDGTLIDASEAIVRAFRITLDADDLPDGA
                     IRSLIGKPLTQMLQQFRPLASPSQIAALVERYRNAFLPLAASHSRPLPGAIETLAFLR
                     GKVHLAIVTTRMSDGAVQMLSAHDMLDAFDLVIGLEHVDHPKPHPQPILRALAILGVE
                     ARRSVMIGDTPDDMKAARDAETGAIGVTTGAFDTRALEKAGAHHVIASLTELDVLLRQ
                     PIEMAHRQRLEFSAP"
     gene            complement(2890..3645)
                     /locus_tag="PK710_16180"
     CDS             complement(2890..3645)
                     /locus_tag="PK710_16180"
                     /inference="COORDINATES: protein motif:HMM:NF012280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor"
                     /protein_id="HOT11320.1"
                     /translation="MSRFTHFACALVAVALGGCAVGEEASSIDPIVELQDTTPPADPP
                     ADDSDRVDPASDDSNAPLYFGTPNELSKDKADNESLSYTFELVKDAYVSIELERRSDK
                     GPRTIGFTLYRIDYKDRIELGEAIGHDGFAAMALYTVHGGLYAIQLFEGIDPDSLVLK
                     LSCNSGTCAPARQPNEVCGNAAGLTCDKGLVCMYEAGTCSAEKRPARCRIEPTECPPN
                     YVPVCGCDGRTWGNECYATQAGVSVLHSGQCES"
     gene            complement(3915..>6456)
                     /locus_tag="PK710_16185"
     CDS             complement(3915..>6456)
                     /locus_tag="PK710_16185"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT11321.1"
                     /translation="GMPDRRIGTVLPGGYVLQQCIGGGGMGQVYRAEQSTLGRSVAVK
                     IVHPYLLSDESVSRRFINEARLASRLNHPNSVAVIDFGKTEDGLPYLVMEHLRGRDLA
                     RVLEEDWPLSFSRIIDILRQLLDVLAEAHQLGIVHLDLKPDNIVLEPTRRGGDFVKVV
                     DFGLARMLTAGTSDTSSPSMTISGTPDYMAPEQCRGEKPDARSDLYAVGVILFLLLTG
                     RLPFEAAAPTQVLLNHLSQPIPDPRVVNPARHIPDELVEVLRRSLQKNPTDRYASAAE
                     FSAALAEAHLLITTPSRALSIPPAAAPSKPCTVCGQPVAIVQKFCGSCGAKMPWADRL
                     PSLRPPSGPADDPHTPQSFPLVERDEQLAVLGQQLDSCATTLHLCRVVGEMGAGRTRL
                     LDEFSMIASTLDHAVFRAHAEPWPLSPAWGTIRELIESLMGADVLTLLENSAVDLRIK
                     QGLELLAHPSTQVDSMPRAHVAHATRWALQTACSHEDLQVVVIVDDLDQLDGASRNAL
                     SDALQLGDIPGLLVIVSHGPDYDPPWLEDKPRVVVEPLTWQSAHSLLASSGVMDIQIS
                     LSERILPLYLEHCIRFEQEGGTAPPPRLGDLIAARFARLPADTRRVLQAISVLGPRIN
                     TQQIQSVTETSTDLSPLVRKLQSVGIVSMVGETICFSHPYLRKIARAVTPAAVLRELH
                     AKAIEQAPGSQPLEVQSFHLEHAGDVFGALLSLDRVGMQARSRDDLDSALEAFRRGFE
                     LARRSAGDPSLDDPMEAVALFGRKLGETLSQKGFHTEAAGILGESLAFASTTEQRARI
                     TFALASVARLRGKPRHAHELAREAIVLLSDTHPSLAASFEQQARWWKA"
BASE COUNT         1462 a         1715 c         1933 g         1346 t
ORIGIN      
        1 gaaaggcgaa ctcatgggct tgcttacgga acgcctggtg gagcgatata ccgtaacgag
       61 acaagagcag gatgagattg cgtttcgcag tcatcacaat gcgctttccg cgtggacgaa
      121 cggatacttc gatgattacg tggtgcctgt ttcgattcct catcgaaaag gtgagccgct
      181 tttggtggcg aaagatgaag gacccaagcc ggtcaccatg gaagcgttgg gtgccgctaa
      241 gccgtatttc aaacccgagg gaggcaccat cacgagcctc aatgccagca gtttgaatga
      301 tgcggccgct gcggtgatgc tcacgacgga agaccgggcg aaagagcttg ggcttgtgcc
      361 gctcgccgag cttcgggcct ttcacaatgt gggtgtggag cgtgaataca tgggggaggg
      421 agcgttcaag gtcatcccgc cgctgctaga aaaaatccga attggtgtgg gtgatgtgga
      481 tctatttgag atcaacgagg cgttcgcggc tgtggtagcg ggcgcatacc acttcatcga
      541 aggacttgcg gtggacaaaa cgaacctttg gggaagtggt atctcgcttg gtcatccggt
      601 gggatgcacg ggagcacggc agattgtcga tatggttcat cagcttcgaa aacgagacaa
      661 aagcgttgga gtcaccacgc gatgcgtggg cggaggcatt ggaagcggcg aagtgctgat
      721 tcgcatgaac tgagagttac caggcgatac cgtggttgac cgacggtctg tcgttcttga
      781 cttcattata aatcaagcgg ttttcatgcc tttcgagcat cacttccggc ccgtgcaaag
      841 acgtcataac cggccgtggc aaggagcgac acaaggcgca tgagaggcaa accgatgatg
      901 gcggtggggt catcgccttg gacccggtcg aatagggcga tgccgaggga ttccagtttg
      961 taagaaccgg cgcaatccaa cggctcatcg atctgcacat agcgttcaat ttgtcgcgga
     1021 tttaatggac gcatcgtcat ttcgtgaaca tccaccacgg tgtcgcagcg tccgctgcgt
     1081 gtatcgagca gcgaaagcgc cgtaaacaag tgatgggttc gcccggacag caggcgcagt
     1141 tgccccatgg ctccttggtt cgttccggcc tttccaagaa cgcgaccatc gagttcgacg
     1201 cattgatctc cgcccacgat gagactgttc gggtgatgat ggccgaccgc ttgtgctttc
     1261 tgttccgcaa ggattcgaac gagttcggaa gggggcagcg ccaaggcctt gaagcgtgtt
     1321 tcatcgatgc ccgagtccac ggcttcaaac gcaatacgaa gtcttgcgag cagttgtttg
     1381 cggtagggag aggtagatgc cagcaccacc ttggggatgg ctcggcaagg atcatcgaca
     1441 ctgtcgttca tgggacgaat tgtggtaggt cctacggtac gatggaagac cccacgaaca
     1501 accctctggt ttggctcgac atggaaatga ctgggctcga gccattgacc tgtgttcctt
     1561 tgcaaatggc cgtggtgatc acggattcgg aactcgagga actcgattcg catgaggtga
     1621 ctctctggca gccggaagaa gtgctgtcac gaatggatcc attcgttcgg aaaatgcaca
     1681 cggaaaacgg actgctcgat cgcgttcgcg cgtcggatgt gggaatgctt caagcggaac
     1741 gcgagatcat ggcgcgcgta tcgcgttatt gccctttcgg tgcgggaatc ctcgcgggca
     1801 actccatcca cacggaccgc gcgtttttga aagcctattt cccaaccctg catggatatc
     1861 tgcactaccg catggtggat gtctcgagtc tcaaagagtt ggttcgacga tggtacggac
     1921 gtgattggct gttcatcaaa cagcttcaga agcatacggc gctcgaagac gtgcgcgaat
     1981 cgatcgcgga gttgcggcat taccgtttga cgtgcatgcg ctcaccgaac aagtcctaag
     2041 ccatgttacg gtgctgagaa ctcgagtcgc tgtcgatggg ccatttcgat gggctgacga
     2101 agcaagacat cgagttcggt cagagaagcg atgacgtgat gggctccggc tttttcgagc
     2161 gcgcgggtgt cgaaagcgcc cgtggttaca ccaatcgcac ccgtttctgc atcacgggcc
     2221 gctttcatgt catcgggggt atcgccaatc atgacgctgc ggcgcgcttc gacaccaagt
     2281 atcgctaagg cacgaagtat gggttgtgga tgcggtttcg gatgatccac atgctcgagt
     2341 ccgatcacca gatcgaaagc gtctagcatg tcgtgagcag atagcatctg cacggcgccg
     2401 tcggacatcc gtgtcgtgac gatagcgagg tggaccttgc cacgaagaaa agctagcgtt
     2461 tcgatggcac ccggaagggg gcgtgaatgc gaagcagcaa gaggcaaaaa tgcgttgcga
     2521 taacgctcaa cgagcgcagc aatttgtgac ggagatgcca agggacgaaa ctgttggagc
     2581 atctgggtga gtggttttcc aatcagactt cgaatggcac cgtcgggtaa atcatcggca
     2641 tcgagcgtga ttcgaaacgc tcgcacgatg gcctcggaag catctatcaa cgtaccatcg
     2701 aaatcgaaaa gcacagcatt cagggctggc ggaaaacagc agggcatggc ggcaccctac
     2761 cgctgggatc gctgggaatg ctagcgcgcc agcagacgaa ttgatgggga tcgtgcggaa
     2821 ataagagaaa acacgattgt cgttacgtcg tgcatccaag agcatcgatg cgccctggac
     2881 gcgttgccct taggactcgc actggcccga atgcagaacg gacaccccag cttgggttgc
     2941 atagcattcg ttgccccaag tccttccatc gcatccgcac acgggaacgt agttcggagg
     3001 acactcggtc ggttcgatgc gacagcgggc aggacgcttc tctgccgagc aagtgccggc
     3061 ttcgtacatg caaacgagcc ctttatcaca cgtcaatccc gccgcgtttc cgcatacttc
     3121 attgggttga cgcgcgggcg cgcaggttcc tgagttgcat gagagcttca acacgagcga
     3181 gtccggatcg atgccttcga acagttggat ggcgtacagt ccaccatgca ccgtatacaa
     3241 cgccatcgcc gcaaatccgt cgtggccgat ggcctctccc aattcgatgc gatctttgta
     3301 atcgatacga tagagcgtaa agccaatcgt tcggggacct ttgtcagaac gtcgctccaa
     3361 ttcaatcgat acatacgcat ccttcacgag ttcgaacgtg taggaaagcg actcgttgtc
     3421 tgctttatcc ttcgacaatt cattgggcgt gccgaagtac agcggggcgt tgctatcatc
     3481 gctggcggga tcgactcggt ccgagtcatc ggcgggcgga tctgccggcg gcgttgtgtc
     3541 ctgaagctcg acaatgggat cgatcgatga ggcttcttcg ccgaccgcgc atccaccgag
     3601 agcgacagcc accaaagcgc aagcaaagtg agtgaagcga gacatggtgc cccctttcga
     3661 atcgggatct ttcgagtacg agaaacggaa cctatgtgac tccgtgtggg gaagaagtca
     3721 accgataata tggtctgtgt tgaagattgc cgtaggcgtt gatttataga atgcagaaac
     3781 tatggcaagt atggtgtaat tcgtaaatcg agaagaaaaa tgcgtgttcc caggctcttg
     3841 gggcaaaggg gcacctgtcg aggtggagca taccttggct gcgtgaaacg cgaccatgac
     3901 cgaccctcga ccgctcacgc tttccaccac cgcgcctgtt gctcgaagga agcggccaac
     3961 gacggatggg tatccgacaa caaaacgatc gcttctcgcg ccaattcgtg ggcatgacga
     4021 ggctttcctc ggagacgagc caccgatgcg agggcaaagg tgatgcgcgc ccgctgctcg
     4081 gtggtcgatg cgaacgccaa gctttctccc aggatacccg cggcttccgt gtggaaacct
     4141 ttttgggaca gtgtctcgcc gagttttcgg ccgaataaag cgaccgcttc catgggatca
     4201 tcgagactcg ggtcgcccgc acttcgtcga gccagttcga atccacgtcg gaaggcttcc
     4261 aaagcactat ccaaatcatc gcggcttctg gcttgcattc caacccgatc caggctcagc
     4321 agggctccga acacatcgcc ggcatgttcg aggtggaacg actggacttc caaaggctgt
     4381 gaaccaggcg cttgctcaat agctttggcg tgcagttccc gcaaaaccgc cgctggtgtc
     4441 actgcgcgag caatcttccg aagataagga tgactgaaac atatcgtctc cccaaccatc
     4501 gatacgattc ccaccgactg cagttttcga accaggggac tcagatcggt cgatgtttca
     4561 gtaactgatt gaatttgttg tgtatttatc cgaggtccca gcacgctgat ggcttgcaga
     4621 acgcgacgag tgtccgcggg aagacgtgcg aaccgcgcgg cgatgagatc tcccaaacgc
     4681 ggtggaggtg ctgtgcctcc ttcttgttcg aaacggatgc aatgctcgag gtatagggga
     4741 aggattcgtt cggaaagcga gatctgaatg tccatgaccc cggagctggc gagcaacgaa
     4801 tgagcggact gccaggtgag tggttctacg accacacgag gtttgtcctc gagccaggga
     4861 gggtcgtaat cagggccgtg ggaaacgatc accagcagac caggaatatc cccaagctgc
     4921 agggcgtccg atagcgcgtt gcgcgaagcg ccgtcgagct ggtccaaatc atcgacgatc
     4981 accacgactt gcaggtcttc gtggctacat gcggtttgca gagcccaacg tgtcgcgtga
     5041 gccacatggg cacgcggcat ggaatcgacc tgtgtgcttg ggtgtgccaa caactccaac
     5101 ccctgcttga ttcgtagatc tacagcggag ttctcgagca aggtgagcac gtccgcgccc
     5161 atcaacgatt cgatcaattc gcggatggtt ccccaagcgg gactcaaggg ccacggttca
     5221 gcgtgagccc tgaacacggc gtggtcgagc gtggaggcga tcatcgaaaa ctcgtccagc
     5281 agccgagtcc ttccggcacc catctcgccc accacacggc atagatgcag ggttgttgcg
     5341 catgaatcga gctgttggcc caacaccgca agttgttcat ctcgttctac gagaggaaaa
     5401 gactggggtg tgtgcgggtc atcggcgggc cccgatggcg ggcgtaggga tggcagccga
     5461 tcggcccagg gcattttggc gccacaactc ccacaaaact tctgtacgat cgctacgggt
     5521 tgtccacata ccgtgcacgg tttgctagga gcggctgcgg ggggtatgga aagggcgcgg
     5581 gacggagtgg tgatgaggag atgagcttcc gcaagggcag cggaaaactc ggccgccgac
     5641 gcatagcgat cggttggatt tttttgtaag gaacgccgca atacctccac caattcatcg
     5701 ggaatatgtc gagctggatt gacgacccgc gggtcgggaa ttggttggga aagatggttg
     5761 agcaggactt gcgtgggagc ggctgcttcg aagggaagac gtcccgtgag cagcaaaaat
     5821 aaaatcacgc cgaccgcgta aagatcactg cgagcgtctg gcttttctcc tcgacattgt
     5881 tccggagcca tataatccgg tgttccggag atggtcatgc ttgggctgct ggtgtccgaa
     5941 gtgcccgcgg tcaacattcg cgcaaggccg aagtcgacga ccttgacgaa gtcaccacca
     6001 cgtcgggttg gttccaaaac gatgttgtcc ggtttcagat cgagatgcac gatcccaagc
     6061 tggtgagctt ccgcgagaac atcgagcagt tgcctcagaa tgtcgatgat tcgtgagaaa
     6121 gataagggcc aatcctcttc gagcacgcgg gctagatccc gtccacgcaa atgctccatc
     6181 accaaatagg gcaaaccatc ttccgtcttc ccaaaatcga tgactgccac ggagttggga
     6241 tggttcaacc gactcgcaag tcgcgcctcg ttgatgaatc gacgacttac ggactcatcg
     6301 ctcagcagat aaggatgaac gatcttcacg gctaccgagc ggcccaaggt ggattgctcc
     6361 gctcgataga cctggcccat tcccccccca ccaatacact gctgaaggac atacccgccc
     6421 ggcaacaccg tgccaatgcg tcgatccggc atgccg
//
LOCUS       DAOSOR010000327         6418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_12188_length_6418_cov_5.291058, whole genome shotgun sequence.
ACCESSION   DAOSOR010000327 DAOSOR010000000
VERSION     DAOSOR010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12188_length_6418_cov_5.291058"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..634)
                     /locus_tag="PK710_16190"
     CDS             complement(218..634)
                     /locus_tag="PK710_16190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOT11322.1"
                     /translation="MLQLQKNLTIPTRPKSIHDVTSLVADVVRQSRVQVGICVVFVRH
                     TSASLLIQENADPSVLEDLEKWMQGLAPESRRWAHDTEGPDDMPAHARCAITRTSETI
                     PITDGRLALGTWQGLYLWEHRAHAHRRQLLVHVMGT"
     gene            complement(933..1100)
                     /locus_tag="PK710_16195"
     CDS             complement(933..1100)
                     /locus_tag="PK710_16195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11323.1"
                     /translation="MMALLEYIHGHIDHDESTVAYLMNTGAILCGFAALACSVCRDRD
                     RDRDRRWEQTR"
     gene            1099..1524
                     /locus_tag="PK710_16200"
     CDS             1099..1524
                     /locus_tag="PK710_16200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOT11324.1"
                     /translation="MTELVPMDGHGTHHMAMAPRRRRNLLHQLDVYRCALQLYQAVRR
                     IVSYFPRGEADLRSQMRRASRSVPFNIGEGVGKRGNARAASYEIALGETKEIIVALDC
                     VEIDKLAPFEEVIAAQDLADRCAAMLTKLIGSVDRGRTP"
     gene            complement(1679..3514)
                     /locus_tag="PK710_16205"
     CDS             complement(1679..3514)
                     /locus_tag="PK710_16205"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOT11325.1"
                     /translation="MSQEIERRAGGPRVPLHTLVEIGAGEGGSAAFEAESVNVTTAGM
                     HLKTAYLPEVGEPLTCRFEGAGTEVVVQGEVAWRTEDSYGGDFGVRFLEMDQVSLDAL
                     RAIAGETIDEQDEHSASDPEQAAMDRGMRVRMHIDELASPMKARVRESTGHEVTVGTN
                     LDILRIGRSLELESVDTQSKRPARVERISVEIEPTSRVPQLIVALRFLDVAAETQPPV
                     METQYAEDAEEEQDEDQELEAESAGVWDKVKKVGPKFAHLGSVARRWGGKAKDAMGDA
                     VVAAKKTAARKVEERKAASAPRRTTAPPPKGGYKGTVLRKQHPDRAEDDLVANEESSL
                     KKYMNKRTAAITVVALLVLLVGGGLARAMFRSNSSEAMAAKEAASASESQEDAPIVPG
                     AMGTDVSAALVANVPLFGPTPMSTAAPMAPMAAVPAPAIGNAALPPGDDATADSDDSA
                     EDSGEESEPSTVAAADDSEGDDDAAEPAPKPAKSSAKSSKKTAKADAPKSFGRGRVSN
                     PVVLTLKMNRKVTGIRGVAHSEGFVVDVMGARSAEPAAGLRRQDSRIAVSKVVNRGSN
                     SQLSVRFKGTPPAYRVQASGSTLRIIVDSDKKTASTQSDKGKKRR"
     gene            complement(3643..5781)
                     /locus_tag="PK710_16210"
     CDS             complement(3643..5781)
                     /locus_tag="PK710_16210"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT11326.1"
                     /translation="MMKAPSLHCAICHTEYPQGSFLHCPRDGSPLHDSQGREIGRLGQ
                     NILDRYQILRFVGKGGMAQVYEAQHAASGRHVAIKILNPRLASNPDVALERFRREAQL
                     LSRIAHPNVVAIEDFGALEDGALFMVMELLRGKTLQQSLDEREPSVAETWEIILQVCE
                     AVQAAHDVGVVHRDIKPANVFLEPPLPSGACVVKVLDFGISKTMGEQRAMNLTKAGSV
                     FGTPEYMSPQQARGEAVDASSDVYSIGVMLYRMLLGTVPFTGDSYLEVLIKHITESPV
                     WPEDIASRRRLPAKAKQVVLTALAKKPSERFPSVDAFAQAISSMRNETTTSYSMVHQQ
                     VDSTRIRSVTRTSSRPPSLSATVSIEHAKGLDGEVVALAPDVFWVGRRTGGALECNAY
                     LRVYHSEEHRFAVLIDPGPPRDLHVIGQKVASVLGSITDVDLLFINHQDPDVAFNAVA
                     LQKINPRVHVVCSEHTWRLIHLMELEPRRYSAVEQFARATMHLVTGDEVRFIPTPYCH
                     FRGAVMYYDVSSRVLFSGDLFGGVSKSRELLSTGEPWDGVDMFHQVYMPSGRAVQQAI
                     ARVRQLDPPPRFIAPQHGAIFGEDTIAALLDHLEQLPMGLDLDSGAEEPGRFVGALTM
                     LIRYLIGIVGKDLVLDRVRRFEGDDTFPAILVLDEQGEITGLRIGARLAAVSVLRTLT
                     EIVPFPYQNEWFDMIERAKRMYDIYLPSIR"
     gene            complement(5784..>6418)
                     /locus_tag="PK710_16215"
     CDS             complement(5784..>6418)
                     /locus_tag="PK710_16215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11327.1"
                     /translation="VVLEGIVTVAPAEGSTLAAIDAWNAFSAPGAGVAVTADPAAASV
                     LFMAYSLDSTIDKSVAALRANDKAVFWDRRPGSFAVQDLKRAEQLMWAQGTSHAWIGF
                     EPDRSPHVFLGALRLTQSDTAPPLDNGWEVLIVLQGTATLALSGERVESNANIARNME
                     LQPGQVVTIPSNVARTVNPGSSQPWYAVQLFVPPGPERRYRDLVQQKEGP"
BASE COUNT         1331 a         1869 c         1874 g         1344 t
ORIGIN      
        1 gacctcgact actccaccac cacgttggaa ctcgtgggag gctttgttgc caccaatggt
       61 cacctgcacg acgaggtatt ggctacggcg cgacgagtgt gggaagaggg ctgatcgctc
      121 acgagggaag ctaattattt acaataattt caattgatta caatcgattg gggaattgct
      181 atcttccgtt cgttctcggg cgacggcaca ccccctgtca cgtacccatc acatgcacca
      241 gaagttgtcg tcggtgtgcg tgcgcccgat gctcccatag atacaatcct tgccaggttc
      301 ccaaagccag acggccgtcg gtgatcggaa tggtttccga ggttcgggtg atggcgcagc
      361 gggcgtgggc tggcatatcg tcgggtcctt cggtgtcatg agcccatcgt cgggactctg
      421 gagccaagcc ttgcatccat ttttccaggt cctcgaggac ggaagggtcg gcgttttcct
      481 ggatgagcaa gctggcggaa gtatgccgca cgaaaacgac gcatatccct acttgaacgc
      541 ggctctggcg aaccacatcg gcaaccaggc tggtcacgtc gtggatcgac ttgggccgtg
      601 tggggatggt caggtttttt tggagttgca acacagggca aggctagtcc ggcggtggtg
      661 tagtgcaagg ggtcgtggag cagggagggc gcattcaatt acgctgtcca tgggcagaaa
      721 actgcgtttc atgctgtgaa agacgtctgt ttcgaactgg acccacgtag cgaccctgaa
      781 tgatgccgga gttgatagca caagccgtcg caagctgatg ggcatcgtgg tcattggcgt
      841 catggtggtg atttcccatg gacggtcacg gccacggcac acgctacacg ccaacgccgc
      901 aaaaccgatg aaagctcgtg cgcctctccc ggtcaacggg tctgttccca tcgacggtca
      961 cggtcacggt cacggtcacg gcacacgcta cacgccaacg ccgcaaaacc gcataaaata
     1021 gcgcctgtgt tcatcaaata cgcaaccgtg ctctcgtcat ggtcgatatg cccgtggatg
     1081 tactcaagga gcgccatcat gacagaactt gttcccatgg acggtcacgg cacacaccac
     1141 atggcgatgg caccacgccg tcggcgaaac ctgttgcacc agctcgatgt gtaccgctgc
     1201 gcactacaac tgtaccaggc cgtccgtcgt atcgtgagct atttcccccg tggcgaggcg
     1261 gacctgcgaa gccagatgcg acgggcctca cgtagcgtgc cattcaatat cggcgagggc
     1321 gtcggcaagc gcggtaacgc aagggctgcc tcctatgaga tcgccttggg agaaaccaag
     1381 gagatcatcg tcgcccttga ctgcgtcgaa atcgacaaac tcgcaccgtt tgaggaagtc
     1441 atcgccgccc aagatttggc ggaccgctgc gctgccatgc tcaccaagct cattggtagc
     1501 gtggaccgtg gccgtacgcc gtgaccgtga ccgtgtccgt gagcgtgctc cgtgagcgtg
     1561 ctccgtgaac ggtgagcgtg aaccgcggcc ataacaacgg ccaccgtaga cacgctttgc
     1621 ctgggtcgtg ggatcttagg gggtaacaga aagacgaaaa ggcgagaccc gactcggatc
     1681 agcgtcgctt tttgccttta tccgattggg tagaagccgt ctttttgtcg ctatcgacga
     1741 tgattcgcag ggtcgaaccg cttgcttgca cgcgataggc ggggggcgtt cccttgaagc
     1801 gcacactcag ttgagaattg gagccacgat tcacgacctt ggacacggcg attcggctat
     1861 cttgccttcg caggcccgcg gcgggctccg cgctgcgagc gcccatgacg tcgaccacga
     1921 agccttcact gtgtgcaacc ccgcgaattc ctgtaacttt tcgattcatc ttcaaggtga
     1981 gaaccaccgg attgctgact cttcctcgcc caaagctttt cggcgcgtcg gccttggcgg
     2041 tttttttgct ggacttggcg gatgatttcg caggcttggg cgcgggctcg gcagcatcat
     2101 cgtcgccctc ggagtcatcg gcagcggcca cggtggacgg ttcgctctct tccccagaat
     2161 cctcagcgct gtcgtcactg tcggcagtgg cgtcgtcacc gggaggaagg gcagcgtttc
     2221 cgatagcagg agcgggaacg gcggccatgg gtgccatggg cgcggccgtg gacatcggtg
     2281 tgggaccaaa aagaggaacg ttggcgacca aggcagcact gacatcggtg cccatggcac
     2341 cgggaacaat cggggcgtct tcttggcttt ccgaagcgga agcggcttcc ttggcagcca
     2401 tggcttccga cgaattggag cggaacatgg cgcgagctag gccgccgcct acgagcaaca
     2461 caagcaaggc tacgacagta atcgcggcgg ttcgcttgtt catatatttt ttgagagagg
     2521 attcctcatt cgccacaagg tcgtcttcag cccggtccgg atgctgcttt cgcagcacgg
     2581 tccctttgta tccgcctttg ggaggcggcg cggtggtacg tcgcggggcc gaggcggctt
     2641 ttcgttcttc gacttttcta gcggccgttt ttttggccgc gacgacggca tctcccatgg
     2701 cgtctttagc ttttccaccc catcgtcgtg cgacagagcc aagatgggca aacttgggtc
     2761 cgactttttt gaccttgtcc caaacgccgg cggattcggc ttcaagttct tgatcctcgt
     2821 cctgttcttc ctccgcatcc tcggcgtatt gcgtttccat caccgggggt tgtgtttccg
     2881 ccgccacatc caaaaagcga agcgcaacga tgagttgtgg aacgcgggac gtcggttcga
     2941 tctcgacgga gatacgttcg accctggcgg gacgtttcga ttgggtgtcg acgctctcga
     3001 gttcgagaga gcggccaatt cggaggatgt cgaggttcgt gcccacagtc acctcatggc
     3061 cggtgctttc acgaacgcgt gctttcatgg gggaagcaag ctcatcaata tgcattcgca
     3121 cacgcatgcc tcggtccatc gcggcttgct ctgggtcgga ggcggaatgt tcgtcttgct
     3181 cgtcgatggt ttctcccgca atagctcgta aggcatcgag gctaacctgg tccatttcca
     3241 ggaaccgcac gccgaagtca cctccgtagc tatcttcggt gcgccaggcg acttctcctt
     3301 gcacaacgac ttctgtgcca gcaccttcga accgacaggt cagcggttct ccaacctctg
     3361 gtaaataggc agtttttagg tgcataccgg cagtggtcac gttgacggac tctgcctcga
     3421 aagccgcgga gccaccctca ccagccccaa tttcgaccag cgtatgaagg gggacccgcg
     3481 ggccacccgc gcgacgctct atctcctggc tcatgcctga atcctccgtg ttggcgtcgt
     3541 cacgtggcta tcccgtgcga accaccattg tggcagggcg tacggcgttc cgggcaaccg
     3601 ggccaagttt tgagatgcaa aacgggtcga ggagcagacc gctcagcgga tggacggcaa
     3661 ataaatatcg tacatgcgtt tggcgcgctc gatcatgtcg aaccactcat tttgataggg
     3721 gaagggaaca atctcggtca gcgtccgcag gaccgacacg gcggccaatc gagcaccaat
     3781 gcgcaacccc gtaatctcgc cctgctcatc caacaccagg atcgccggaa atgtatcgtc
     3841 gccctcgaat cgccgcaccc gatccaacac gagatccttt cctacaatac cgatgagata
     3901 gcggatgagc atcgtgagag cgcccacgaa tcgccccggt tcttcggctc cgctatcgag
     3961 atccaggccc atcgggagtt gttcgagatg atcgagaaga gccgcgatgg tgtcttcgcc
     4021 aaaaatggcg ccgtgttggg gagcgatgaa tcgggggggc ggatcgagtt gccgaacgcg
     4081 agcgatggct tgttgcacag cgcgaccgct gggcatgtaa acctgatgaa acatatcgac
     4141 gccatcccag ggctcgccag tggacagcaa ttccctggat ttcgagactc ctccgaatag
     4201 gtcgccggaa aatagcaccc tcgacgagac gtcgtaatac atgaccgccc ctcggaaatg
     4261 gcagtagggc gtgggaatga atctcacctc gtcgcccgtc accaaatgca tcgtcgcacg
     4321 agcaaactgc tccaccgcgc tgtagcgtcg cggttccaat tccatgagat gaatcaagcg
     4381 ccaggtatgc tcagagcaga cgacatgaac gcgcggattg attttttgta acgcgaccgc
     4441 gttgaaagcc acgtccggat cttgatgatt gatgaacaac aggtccacgt ccgtgatcga
     4501 tccaagcacg gaagcgacct tttgcccgat gacgtgcaga tcccgcggtg gtcccggatc
     4561 gatgagaacg gcaaaccgat gttcttccga atgataaacg cgaagatacg cgttgcattc
     4621 gagcgcgcct cctgttcgtc gccctaccca aaacacatcc ggggcaagcg caaccacctc
     4681 cccatccagc ccttttgcgt gttcaatgga aaccgtagcg gacaaagaag gaggcctgga
     4741 agacgtgcgc gtgacgctac gtatccgcgt ggaatccacc tgctgatgga ccatcgaata
     4801 cgatgtcgtc gtctcgttgc gcatcgatga aatcgcttgc gcaaacgcat cgaccgaagg
     4861 aaatcgttcg cttggtttct tggcgagggc ggtcaaaacc acctgtttgg ccttcgcggg
     4921 aagtcgacgt cgtgacgcga tatcctcggg ccacacggga gattcggtga tgtgcttgat
     4981 caacacctcc aggtacgagt caccggtgaa aggcacagtg cccaggagca ttcgatagag
     5041 catcacgccg atggaataga catccgaact cgcatcgaca gcttctcctc gcgcttgctg
     5101 gggagacata tactcgggcg taccgaagac cgaaccggct ttggtcaggt tcatcgcgcg
     5161 ttgttcgccc atcgttttcg aaatcccaaa atccagaact ttgacgacac acgctcccga
     5221 aggcagaggg ggttcgagaa agacgttggc gggtttgatg tctcgatgca cgacgcccac
     5281 atcgtgcgcg gcttgcacgg cctcgcagac ttgcagaatg atctcccacg tctctgccac
     5341 cgatggctct cgctcatcga gggattgctg caatgttttc ccacgcagca attccatgac
     5401 catgaacaag gcgccatctt ccaaagctcc aaagtcctca atcgctacga cattgggatg
     5461 agcgatgcgc gacaatagct gagcctcacg tcgaaatcgt tcgagcgcga cgtcgggatt
     5521 cgaggcaaga cgaggattga gaatcttgat cgctacatga cgtccggagg ccgcatgctg
     5581 cgcttcgtag acttgcgcca ttcctccttt gccaacgaac ctgaggattt gatagcgatc
     5641 caggatgttc tgtccgagcc tcccaatctc cctgccttgg ctatcgtgca gcggacttcc
     5701 atctctgggg cagtgaagaa aggaaccttg cggatactcc gtatgacaaa tggcgcagtg
     5761 caaagagggc gccttcatca tgattaaggc ccctcttttt gctgcacgag atctcggtat
     5821 cgacgctcag ggccgggtgg tacgaacaac tgcacggcat accacggttg tgaggaaccg
     5881 ggattcacgg tgcgtgccac gttcgaaggg atggtcacga cctgtcctgg ttgcagctcc
     5941 atgtttcggg caatgttggc attcgattcc acccgttcgc ccgacaaggc aagggtagcc
     6001 gtaccttgca agacgatgag cacctcccaa ccgttgtcga gcggcggtgc cgtatcggac
     6061 tgcgtgagcc gaagagcgcc cagaaatacg tggggagatc gatcaggctc gaaaccgatc
     6121 cacgcgtgag acgtgccttg cgcccacata agttgttcag ctcgcttcaa gtcctgcacg
     6181 gcgaagcttc ccgggcgtcg atcccaaaac acggccttgt cgtttgcccg cagtgccgcc
     6241 acagatttgt cgatggtgga atcaagggag taggccatga acaacaccga ggcagcggcc
     6301 ggatcggcag tcaccgcaac gcctgcgcca ggagcgctga acgcattcca cgcatcgatg
     6361 gcggcgagtg tggagccttc ggcgggcgct accgtcacta tcccttcaag gacaaccc
//
LOCUS       DAOSOR010000328         6376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_12294_length_6376_cov_4.326531, whole genome shotgun sequence.
ACCESSION   DAOSOR010000328 DAOSOR010000000
VERSION     DAOSOR010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12294_length_6376_cov_4.326531"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1774
                     /gene="aceE"
                     /locus_tag="PK710_16220"
     CDS             <1..1774
                     /gene="aceE"
                     /locus_tag="PK710_16220"
                     /inference="COORDINATES: protein motif:HMM:NF036501.2"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HOT11328.1"
                     /translation="DALLERDPDQILVQRMNEIVDGQFQKYSIESGDFIRADFFGKDP
                     RLLELVKHLSDDDIRRLRRGGHDMRKVYAAFHAAVQHQGAPTVILAHTVKGWTLGPGV
                     EAKNVTHQQKKLDEDELRAFRNRLELDIPDRQLKDPPFFHPGMDSEEVQYMLERRRAL
                     GGFIPQRAARAYSLPMPDEQKDPFEEFFAGSKAEVSTTMAFARMFSKLLRDKGIGKHV
                     VPIIPDEARTFGMDPLFSQIGIYAAHGQAYEPVDAGLMLKYRESATGQLLEEGITEAG
                     SVASFTAASTSYATHGVPMIPFYMFYSMFGFQRTGDQMWALGDIRARGFLMGGTAGRT
                     TLMGEGLQHADGHSHLLASTIPAIRSYDPAWAYELAVIVRHGLQEMFVEQKDEIFYIT
                     IHNEAYEMPPMPEGVQEGIIRGLYRFRPAAEPRTHRAQILASGPIMREALRAQQILAE
                     KYDVAADVWSATSYLALRREALEIERWNRHHPDQPARVSYVASLLDATEGPVICVSDW
                     MKAVPDQIARWVRPPFVSLGTDGFGMSDTREVMRRHFEIDAGSIIIATLHQLFNQGAV
                     SAQVVRQAMEELEYDPEKLDPMSL"
     gene            1843..2490
                     /locus_tag="PK710_16225"
     CDS             1843..2490
                     /locus_tag="PK710_16225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11329.1"
                     /translation="MVGKSKWVALFFASMMLVHCGANDPAPPRSHPTPVDDAGSEAGY
                     PEGPYGVSNGQVIANLSWEGWREPKQSGFEPSAFERISLGDFFDPDGEKGFRAIFVNA
                     SARWCSICKMEQRHIREKRDVWGPRGVVFLETLFENTGSGPATPSDLVAWGNTYQIDW
                     ILVLDPTNKLSSFFDRSASPMNMIIDARTMVIRDIITGMPDESWWAHHLEPLVTP"
     gene            2515..3135
                     /locus_tag="PK710_16230"
     CDS             2515..3135
                     /locus_tag="PK710_16230"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOT11330.1"
                     /translation="MQKRVNPRPYRFGTGMLALVLASGWLACGAPAVSGKDPGVGEST
                     SQTQSSDSAANSAAKPKAASKKIADFSLETVDGKTVSLSDYVGNNVVMLDFWATWCEP
                     CLAAMPHLNEIYRRHKSEGFVLLSISMDGPDTVAEVRAYCQRHKLAFPVLLDEESRAV
                     SLYNPKRSAPFSVLIAKDGTILHKRDGYQAGDESAIEAEIVEALGQ"
     gene            complement(3147..4079)
                     /locus_tag="PK710_16235"
     CDS             complement(3147..4079)
                     /locus_tag="PK710_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HOT11331.1"
                     /translation="MATSLLALLDDISTVLDDVALLTKVAAKKSTGVLGDDLALNAEQ
                     VSGVRADRELPVVWAVAKGSLKNKAILVPAALLIQWVAPWLISPLLMAGGTFLCFEGA
                     EKIAHQFLSRQQDKPSPIERMQSRLSLSQDITAMEKAKIKGAIRTDFVLSAEIVVITM
                     GTVADASIWTSLSVLSGVAVLMTVGVYGVVAVIVKLDDAGRYLSERTGEGVVLGAVRW
                     IGFTVLRLAPYLMKTLTIVGTAAMFLVGGGIIVHGISAVHSFVQFAKDSLGTVFAPIL
                     EPLLQGLMGVLVGMVALLLMSAMQAVIRRVRKSA"
     gene            complement(4133..4618)
                     /locus_tag="PK710_16240"
     CDS             complement(4133..4618)
                     /locus_tag="PK710_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11332.1"
                     /translation="MTTRPLFSIAAFLLLLLVGCTKNYIPNTDVEDTEDNRRVISFCE
                     AYRKAVERRDVGKILSMVSSQYYEDGGNVDASDDMDFEGLKAFLMKEFRESKAIRYEI
                     RYRRVLWSKESDRLFVDYTYSASFKIPGHEDKDEWRRIVAENRLELLVMGDKFQIVAG
                     L"
     gene            4754..>6376
                     /locus_tag="PK710_16245"
     CDS             4754..>6376
                     /locus_tag="PK710_16245"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT11333.1"
                     /translation="MTAEYPVLVVDDDNVSRRVIENVLRHAGIAAVSVAGGNEALFWL
                     ETNKTSLVLLDLIMPPPDGYQVLSELRAHENGADIPVVVLTGVASDEEVSRAFEAGAD
                     DFVRKPFRAAELVARIRGQLRLRSYIEALAKKEKDAQVVLELTQALASSLDFRQILFT
                     VVQRVADVARVNRCSIVLVQGDVGQVAATSDNEDLFNLSIQLDRYPEIRKVVDSGKPL
                     IIENVTTHPLLEVVRDKLPVSELTSLVLVPIGYEGTSMGVLVLRAAARIHLGDHEMNI
                     AQTVANATAIALRNAHVIKNLQDQTEQVSHARVKAERRLQMLKRYAAFFEAGADGVIV
                     TDLDGQILFTNARARSITGFSETALRGRKVDELVTEVDRPRMSEVQAGFAKGSFLQSV
                     DVQVQRSDGQQVVLSINFSGVLREEGAVLMSFRDVTPERTVEAQLRQTKDFLERVIDS
                     SANAIISIDMHGRVQLFNRAAERCLGRTADQMIGRAHIFDFFPKEEAEAIVRMLQQGE
                     EGASISDHSAQVIVDSGELAPVTSSAALIHDDG"
BASE COUNT         1462 a         1809 c         1739 g         1366 t
ORIGIN      
        1 ggatgctctt ctcgaacgag acccggatca aatcctcgtg cagcgaatga acgagatcgt
       61 cgatggccaa ttccaaaaat actccatcga gtcgggagac ttcattcgcg cggacttctt
      121 tggcaaagac ccacgcttgc tggaactggt caaacatctt tccgatgacg atattcgccg
      181 gttgcgacgg ggtggtcacg atatgcgaaa agtgtacgcg gccttccatg cagcggtgca
      241 gcatcaaggc gctcccaccg tcatcctcgc ccatacggtc aaaggatgga cccttgggcc
      301 cggtgtcgag gccaagaacg tcacccatca gcagaaaaaa ctcgacgaag acgagttgcg
      361 agccttccga aatcgtctcg aactcgatat ccccgaccgg cagctcaaag acccgccttt
      421 cttccatcca ggcatggata gcgaagaggt gcaatacatg ctcgaacggc gacgggcgct
      481 tggcgggttc attccccagc gcgcggctcg tgcctattcc ttgcccatgc ccgatgagca
      541 aaaggatccg ttcgaggaat ttttcgcggg ctccaaggcg gaagtgtcca ccaccatggc
      601 cttcgcgcgg atgttttcca aactgctccg tgacaagggc attggcaagc atgtggtgcc
      661 gatcattccc gatgaagccc gaacgttcgg catggatcca ctgttcagcc agatcggtat
      721 ctacgccgcc cacggacagg cgtatgaacc cgtcgatgcc ggtttgatgc tcaagtatcg
      781 ggaaagcgcc accggccagc ttctcgaaga aggcatcacc gaggccggat cggtagcctc
      841 gttcaccgcg gcctccacgt cctacgctac ccacggcgtg cctatgatcc cgttttatat
      901 gttctattcc atgttcggct tccaacgcac cggagaccag atgtgggctc tgggtgatat
      961 ccgcgctcgt ggatttctca tgggaggaac agccggacgc accacgctga tgggggaggg
     1021 attgcaacat gccgacggtc acagccatct gttggcttcc acgatccccg ccatacgctc
     1081 ctacgatccc gcctgggctt atgaacttgc ggtcatcgtg cggcacgggc ttcaagaaat
     1141 gttcgtggaa caaaaagacg agatctttta tatcacgatc cacaacgaag cctacgagat
     1201 gcccccgatg cccgaaggcg tgcaagaagg catcattcgt ggtctgtata gattccgtcc
     1261 cgcggcggaa ccgcgaacgc accgggcgca aattctcgcc agtgggccca tcatgcgaga
     1321 ggcccttcgc gcacagcaga tcctggcgga aaagtatgac gtggcagcgg atgtgtggag
     1381 cgccacgagc tatcttgcct tgagaaggga agcgctggaa atagaacgct ggaaccgtca
     1441 tcatcccgat caacccgcgc gcgtttccta tgtcgcctcg ttgctcgatg ccaccgaagg
     1501 tccggtgatt tgcgtttcgg attggatgaa agccgtcccc gatcaaatcg cccgctgggt
     1561 gcgacctccc ttcgtttctt tgggcaccga tggcttcggc atgagcgata cccgtgaggt
     1621 catgcgaaga cacttcgaaa tcgatgcggg atccatcatc atcgcaactc tgcaccaact
     1681 cttcaatcaa ggagccgtga gcgcgcaggt ggttcgccag gcgatggaag agctggaata
     1741 cgatccggaa aaactcgatc cgatgagtct ctgatcccat ggcgggaagc ggcggttcgt
     1801 gttgcgcctt ggcgacaccc gacgtagatc caagaggagt tcatggttgg caaaagcaag
     1861 tgggtagcgc tctttttcgc atcgatgatg cttgtgcatt gtggcgcgaa cgacccggcg
     1921 ccgcctcgca gtcatccaac accggtggac gatgccggct cggaagctgg ctatcccgaa
     1981 ggaccttatg gggtttccaa cggccaggtg atcgcgaact tatcctggga aggttggcga
     2041 gagccgaagc aaagcggttt cgaaccgtct gccttcgaga gaatctcgct cggtgacttc
     2101 ttcgatccgg atggagaaaa agggtttcga gcgatcttcg tcaacgcttc ggcaagatgg
     2161 tgcagcatct gcaaaatgga gcagcgtcat attcgcgaaa aacgcgatgt ctggggacct
     2221 cggggtgtcg tgttcctcga aaccttgttc gagaatacgg gctcgggtcc tgccacgcct
     2281 tccgatctcg tggcttgggg aaatacctat caaatcgatt ggatcctcgt tctcgatcca
     2341 accaataaat tgagttcctt tttcgatcga tccgcaagtc cgatgaatat gatcatcgat
     2401 gcacgcacga tggtgattcg cgacatcatc accggtatgc ccgatgaatc ctggtgggcc
     2461 caccacctcg aacccctggt aactccgtga cccatgattt cgtggagaaa aaacatgcaa
     2521 aagcgtgtga atccaaggcc ctatcgtttc ggtacgggga tgctcgcact cgtgttggct
     2581 agtggatggc tcgcttgcgg tgccccggca gtgtcgggca aagaccccgg ggtaggggag
     2641 tccacaagcc aaacacagtc ttccgattcc gctgcgaatt ccgcggcgaa acccaaggcc
     2701 gcttcgaaaa aaatcgcgga tttttccctg gaaaccgtcg atggcaaaac ggtctcctta
     2761 tccgattatg tgggaaacaa cgtggtcatg ctcgactttt gggcgacatg gtgcgaaccg
     2821 tgtctcgcgg cgatgcccca tctcaacgag atctatcgaa ggcataaaag cgaaggtttc
     2881 gtgttgctga gcatttcgat ggacggtccg gatacggttg ccgaagtacg agcctattgc
     2941 caacgccata agttggcctt tcccgtgctg ctcgatgaag agtcccgtgc cgtatcgctg
     3001 tataacccca aacgctccgc ccccttcagc gtgttgattg ccaaagacgg caccatcttg
     3061 cacaaaagag atggttatca agccggggat gaatccgcca tcgaagcgga aatcgtcgaa
     3121 gccctcggtc aatgagggca cgatgctcat gccgactttc gtacgcgtcg gataacggct
     3181 tgcatcgcac tcatcaacaa taacgctacc atgccaacca atacccccat cagcccttgt
     3241 agtaacggct cgaggatcgg tgcgaacacc gttcccaacg agtctttggc gaattgcacg
     3301 aaagaatgca cggcagaaat accgtggaca atgattcctc cccccaccaa aaacatcgcg
     3361 gccgtgccca cgatggtcaa cgtcttcatc aagtagggcg cgagccgcaa tacggtgaaa
     3421 ccaatccagc gtaccgcccc aagcacaact ccttctccgg ttcgttcact caagtagcgc
     3481 cctgcatcat ccagttttac aatcacggcc accacaccat acacacccac cgtcatcaat
     3541 accgccacac ccgagagaac cgacaaactc gtccatatgc tcgcgtccgc gaccgtgccc
     3601 atggtgatga cgacaatttc cgcggatagc acgaagtcgg ttcgaatggc ccctttgatt
     3661 ttggcctttt ccatggccgt gatgtcttgg gatagagaca gacggctttg cattcgttcg
     3721 atgggggaag gcttgtcttg ttggcgcgac aggaattgat gagcaatttt ctcggcacct
     3781 tcgaagcaga ggaaagttcc ccccgccatc aaaaggggcg agatgagcca tggtgccacc
     3841 cattgaatca acaaggccgc gggtaccaat atcgccttgt ttttgaggga tcctttcgcg
     3901 accgcccaca cgaccggaag ttcccgatcc gcacgaactc ccgatacttg ctcggcgttg
     3961 agcgctagat catccccaag aactcccgtg cttttttttg cagctacctt cgtaagaagg
     4021 gcaacgtcat cgagaaccgt cgagatatcg tcgagtaacg caagaaggct tgttgccatg
     4081 ccaagcccat gcacccgtga gcaccggact gcaagtctgc ggctcgcctg attcagagac
     4141 cagccacgat ttggaatttg tctcccatca cgagcagctc caatcggttt tccgccacga
     4201 tgcgtcgcca ctcgtctttg tcttcatgcc ctggaatctt gaaactggcg gaataggtgt
     4261 agtccacaaa taaacggtcc gactccttgg accaaaggac gcgtcgatat cgaatttcgt
     4321 aacgaattgc cttggattca cgaaattctt tcatcagaaa cgctttcaat ccctcgaaat
     4381 ccatgtcgtc ggaagcatcc acattgcccc cgtcttcata gtattgagag ctgaccatgg
     4441 ataagatttt gcctacgtca cgacgttcca cggcctttcg ataggcctcg caaaacgaga
     4501 tgacgcgacg attgtcctcg gtgtcttcga cgtcggtgtt gggaatgtaa tttttggtgc
     4561 agccgaccaa aagcagcagc aaaaacgcgg cgatggaaaa caagggacgg gtggtcatga
     4621 tgatgcggcg tcaacagtcc aaacggtcta atgattcctg ggaatgtggc tacgatgccg
     4681 tcagttgcgc aaaagcgcaa ggagcacgtc gggccgtttg caatttccgg cgaacaagga
     4741 ataatctgta acagtgaccg ccgaataccc cgtgctcgta gtagatgacg acaacgtcag
     4801 tcgtcgtgtc atcgaaaacg tgcttcgaca cgcggggatt gcggccgtga gcgtggccgg
     4861 gggcaacgag gcgttgtttt ggctcgagac caacaaaaca tcgctggttt tgctggatct
     4921 gatcatgcct ccccccgacg gttaccaggt actttccgaa ttacgcgcgc acgaaaatgg
     4981 agcggatatt ccggtcgtcg tgttgacggg tgtggcttcc gacgaagaag tcagtcgggc
     5041 ttttgaagcc ggggcggatg atttcgtccg taagccattt cgagcagcgg aactggtcgc
     5101 gcgcatccgt ggacaacttc gcctgcggtc ctatatcgag gccctcgcga aaaaagaaaa
     5161 agatgcgcaa gtcgttctcg aactcaccca ggccctcgcc tcttccctgg attttcgaca
     5221 aatcctgttc accgtcgtgc aacgcgtggc ggatgtggcc cgcgtgaatc gttgcagcat
     5281 cgtgttggtg caaggggatg tcggacaagt cgctgccacc agcgacaatg aagacttgtt
     5341 caatctttcg atacaactag atcgttaccc cgagattcga aaggttgtgg attccggaaa
     5401 gccgctgatc atcgagaacg taaccacaca tccgctgttg gaagtggtgc gtgacaaact
     5461 gccggtcagc gaactcacct ccctggttct cgtacccatc ggttatgaag gaacgtcaat
     5521 gggcgtgctc gttcttcgtg cggccgcgcg tatccacttg ggcgaccacg agatgaatat
     5581 cgcccagacc gtggccaacg ccacagccat tgcgcttcgt aacgcccatg tgatcaaaaa
     5641 cctgcaggat caaacggagc aggtgtccca tgcccgcgtg aaagcagagc gtcgcctcca
     5701 aatgctcaaa cgatacgcgg cgttttttga ggcaggcgcc gatggcgtca tcgtgaccga
     5761 cctggatgga caaatcttgt tcaccaatgc ccgtgcccgt tcgattaccg gtttttcgga
     5821 aactgccctg cggggaagga aagtagatga attggtcacg gaggtcgacc gtcctcgaat
     5881 gagcgaagtc caagcgggat tcgcgaaagg aagctttctg caaagtgtcg acgttcaggt
     5941 gcagcgctcc gatgggcaac aggtggtgct gagtatcaat ttcagcggag tcctacggga
     6001 agaaggcgcc gtgttgatga gcttccgcga tgtaaccccc gaacgcacgg tcgaagcgca
     6061 actgcgtcag accaaagact ttctcgaacg tgtcatcgat agctccgcaa acgcgatcat
     6121 ttcgattgat atgcatggcc gggtgcagct tttcaatcgc gctgccgaac gctgcctcgg
     6181 aagaaccgcc gatcagatga ttggacgcgc tcatatattc gactttttcc ccaaagaaga
     6241 agccgaagcc atcgtgcgca tgctgcagca aggcgaggaa ggcgcaagta tttccgatca
     6301 ttcggcccaa gtgatcgtcg actccggaga acttgccccc gttacctctt cggctgcgct
     6361 cattcacgac gacgga
//
LOCUS       DAOSOR010000329         6329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_12408_length_6329_cov_4.632770, whole genome shotgun sequence.
ACCESSION   DAOSOR010000329 DAOSOR010000000
VERSION     DAOSOR010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6329
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12408_length_6329_cov_4.632770"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /gene="rpsL"
                     /locus_tag="PK710_16250"
     CDS             complement(<1..231)
                     /gene="rpsL"
                     /locus_tag="PK710_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOT11334.1"
                     /translation="MPTINQLIRSGRRMPQAKTTAPALQSCPQRRGVCVRVYTTTPKK
                     PNSALRKVCRVRLTNGIEVTSYIPGEGHNLQEH"
     gene            446..1132
                     /locus_tag="PK710_16255"
     CDS             446..1132
                     /locus_tag="PK710_16255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT11335.1"
                     /translation="MTPRHRLLLVDDDVVFCRSLARSLTHHGFDVLIANTAAEALASL
                     RNHPIDIILLDWILPDHDGVALLQDFRERGIIAPVIMLSAEQSVPGRVRALNRGADDF
                     ISKPFEVEEVIARVHANLRRQTTLYHVTRVGRVSVDAWKQEAMVDGQRVDLTPLEFSL
                     LSYLMRSAGETKSKQQILETVWGDKGAEGSLRALDLQLLRLRRKLGTAASQIETVRGS
                     GIRFIDGNDA"
     gene            complement(1110..2294)
                     /locus_tag="PK710_16260"
     CDS             complement(1110..2294)
                     /locus_tag="PK710_16260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11336.1"
                     /translation="MNARSLLVVCLIGTAVFSSRPSDACAPQMCVGGQFLPAQGAVIP
                     ANTPAIWWWPDRPNGTPDTALPIHGGSFDLMLVDQGNEELLPVHSEPGPAFGGLFLVP
                     KDPMPAGRTIRVRGVSYCPNDTGLLDETATSAEFETTQPADFPTVLGTLVATPQEIGP
                     LTLADASGSCFSTVEAAIVRISLQYASEAMPWKDVFAFETRIDGKPWKPSHFLAREPI
                     IGSSWEGRGQDLAFAICDVPPFSYPAVEPLKPGSHQVTMRAWIPGSNTLLETPGITIS
                     LSCDDADAGNEDAGKEDAGNEDAGDVSFDVEPDVSSDVPPEIDSDAGADTASDAVVES
                     ASDSATDSGADTTNAESSTTDDSDGCSCSMVGRAAPTGVVWVSLWLLGLGIWRFRHRC
                     HR"
     gene            2443..5436
                     /locus_tag="PK710_16265"
     CDS             2443..5436
                     /locus_tag="PK710_16265"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOT11337.1"
                     /translation="MDPITRLFAMVSFVPLDRIQRLISFFAALVCLLFVATLRDDSQL
                     FLVVQTLRTAAVPQNVGPTDGKLLVRVVDPNRAPMPNARVTVLLIRDDIAYLAGFRVT
                     DPQGAVSFEGLAHGILWILAEADERARASSQLVMTSEPKEITMHLGPSHALTIEVVDD
                     DDAPIANAEITLACSDPLPFVANTDDTGHVTIRRLCPPPYVLKATAPGFESTSRTNVM
                     PNSPATRVSLRKLGWIEVNVIDRNKKPAPLSTIFVAGPAIWPARQVQSDASGYSKISA
                     LPAGAYDLRATRQSLVSPISTGIMVERGKGTPVTLTLDEGRMVTVSVTGGLADDAPAV
                     ASANVVLVEGGLSSFPLQGRTGLDGTVQLGPFPPVPLSASARAEGFVSPGVVLVGAND
                     TQVRIALLKAGKLTGEVVDNRGFPVPGCSIEVIGTDLAGLPIAETPAAMAFRRAHFAW
                     AIHGPPTLIPAGELGVMPGPIPPIPRGDISLDSRLPSFEENRDSQQLTPWVTDGHGRF
                     SVSPVPPGRISAIVRHPAHVEAISEAVTLAPGGTAHVRVVLHLGGTLEGVVLDDRRFP
                     ISGAWVRVTAKEGSSERTSMTAMDGSFAFTAVAPVVIVTASRPESPDKVAYQETLSVA
                     AEERKQVEIVLARERPAMTVRVTDDRGYPIDRVHIRAVSLTTDVSLRRTSFSDSKGNA
                     IIEEAAGIRLRLEVSANGYGTHVETFDTSPEMVEVRLDRGLRVQGEVTSRAGRDRVQG
                     ATITAYLPTGTRQVTTNENGMFRLDDLSSGPLRIRVDHESYVSLDKTFEVNTPSDVER
                     PVQLEPMALELAGIVEGEVVDDRGNPVAGARVSKDQVPEYLPVGPLPPGVVVTDEKGK
                     FRLGGLAEGEITLEAFAPGIGRGKEERIAVRKERTTTRVRIRLDPTRSKAGPAIVAGV
                     AVTLAPSTVGPGALVTGVASGSEADRAGIRIGDYIERIDGVVPDDERDAMQRLRGPER
                     QDVIVDIQRENKHLTIRLPRERARQ"
     gene            complement(5489..5932)
                     /locus_tag="PK710_16270"
     CDS             complement(5489..5932)
                     /locus_tag="PK710_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11338.1"
                     /translation="MRRPSSPPELIADFIRERDNGYRIVAAVKKGSKEAPLMYAIQKL
                     CLTSCTGFVVAMLSALAGFGYLIYKLIDWDSFRGGMAPVVIGLIFFSSVQIVILDLLG
                     TFHTGRRNHKITEEQAITKSLDSQGYTSSPALPHARAPDPQLGST"
BASE COUNT         1341 a         1823 c         1797 g         1368 t
ORIGIN      
        1 gtgttcttgt aagttatgcc cttccccagg aatataactc gtgacttcga tcccattggt
       61 caatcgaaca cggcatacct tgcgaagcgc cgagttcggc ttcttggggg tcgttgtata
      121 tacacggacg catacacccc gacgctgcgg gcacgactgc aaagccggcg ccgtcgtctt
      181 ggcttgaggc atcctacgtc ctgatcggat caactgattg atggtcggca tggaactggc
      241 tatcgttagg taatcccgac gcgcaaaaat acgtcggccc aaaagggaag cgcaaaatag
      301 cgatatttgc cgatgtgtca agcgaaagat cattgcccct cccccctctt cactttcgtc
      361 gtttgaatac atccacacac ttgtttcacg cccctttggt tcatatcgac cacaacctcc
      421 taatttttcg tgataattcg cccgaatgac ccctcgtcac cgattgctgc tcgtcgacga
      481 tgacgtcgtc ttttgtcgca gtctcgcccg aagcctgact catcacggct tcgatgtgct
      541 catcgccaat accgccgccg aagcactggc gtccctgcgg aaccatccca tcgacatcat
      601 cctgttggat tggatcctgc ccgatcacga cggcgtggct ctgctgcaag acttccggga
      661 aagagggatc attgcccccg tcatcatgct cagcgccgag caatccgtcc caggtcgcgt
      721 tcgcgccctg aaccgcggcg ccgatgattt catctccaaa cctttcgagg tcgaagaagt
      781 catcgcccgc gtccacgcaa acttgcgaag acaaaccacg ctctaccatg tcacacgggt
      841 aggccgcgtc tccgtcgatg cttggaaaca ggaagccatg gtcgatgggc aacgggtgga
      901 cctcacacct ctcgagtttt ccttgctctc ttatctgatg cgaagtgccg gagagacgaa
      961 atccaaacag caaatcttag aaaccgtgtg gggagataaa ggcgccgagg gctctctgcg
     1021 ggctctcgat cttcaactgc ttcgattgcg aagaaaactc ggaaccgccg ccagccaaat
     1081 cgaaaccgtg cgcggttcgg gtatccgttt catcgatggc aacgatgcct aaaacgccaa
     1141 attcccaaac ccagtagcca cagactcacc caaaccacac ccgtgggtgc cgcacgcccg
     1201 accatggaac aagaacaccc atcggaatcg tcggtcgtag acgattccgc gttggtggtg
     1261 tccgctccac tgtctgttgc tgaatcactc gctgattcta cgacagcatc cgaagccgta
     1321 tccgcgccag catcgctgtc tatttctggt ggcacgtcgc tcgaaacgtc tggctcgacg
     1381 tcgaaagaca catcacccgc atcctcgttg cccgcatcct ctttgcccgc atcctcgttg
     1441 cccgcatccg catcgtcaca ggacaacgaa atcgtaatcc caggagtctc gagcagcgtg
     1501 ttcgagcccg ggatccaagc gcgcatggtg acttgatgac tccccggttt cagcggctcc
     1561 acagccggat aagagaatgg cgggacgtcg caaatcgcaa acgcaaggtc ttgacctcgt
     1621 ccttcccaac tcgacccaat gatcggctca cgagcaagaa aatggcttgg cttccagggt
     1681 ttgccatcga tccgtgtctc aaacgcaaat acgtctttcc atggcatcgc ctccgaagcg
     1741 tattgcaacg aaatcctcac gatggctgcc tcgacggtcg agaagcatga accggaggca
     1801 tctgccaacg tcaaaggtcc aatttcctga ggcgtggcca ccaatgtccc gagaactgta
     1861 ggaaaatcag cgggttgcgt tgtttcgaat tccgccgacg tggccgtctc atcgagcagt
     1921 cccgtatcgt ttgggcaata gctgacgcca cgtacacgaa tcgtgcgccc cgcgggcatg
     1981 ggatctttcg gcacgagaaa caaaccacca aaagcaggac ctggttcact atgaaccggc
     2041 agcaattcct cgttcccttg atcgacgagc atgagatcga aactcccccc atggattggt
     2101 agggccgtat cgggagtgcc gttgggacga tcgggccacc accaaatcgc gggcgtgttt
     2161 gcgggaatga ctgccccttg cgccggtaga aactggcctc ccacacacat ttgtggcgcg
     2221 caagcatcgc ttggacgcga ggaaaagact gccgtgccga tcaaacagac aaccagaagc
     2281 gagcgagcgt tcatgagaca gaaggttacc gcgaatcagg atgccttcga gaactccatt
     2341 tcatttcgat gagggctgtc gaacaaaatt aatcaacgta ttcgcggtgt taccaccgac
     2401 tcgcccctgg ggaacgcggt ccttttcggc ctaaacgaaa ccatggatcc cataacccgg
     2461 ttgttcgcta tggtgtcttt cgtgccgctc gaccgtatcc aacgcctgat atcgtttttc
     2521 gcggcgctcg tatgcctgct gttcgtcgcc accttacgcg atgactcgca attgtttctc
     2581 gtcgtgcaaa cacttcgcac ggcggcggtc ccgcaaaacg tcgggccgac agatggaaaa
     2641 ctgctcgttc gggtggtgga tccgaatcgg gctcccatgc cgaatgctcg cgtgaccgtg
     2701 ctgctcattc gagatgatat cgcctatctc gctggttttc gtgtgaccga tccgcaagga
     2761 gcggtgtctt tcgaaggtct tgctcatggc atcctttgga ttttggcgga ggcagatgaa
     2821 cgggcacgcg cttcgagtca actggtgatg acctcggaac ccaaggaaat cacgatgcat
     2881 cttggccctt ctcatgcgct tacgatcgag gtcgtcgacg atgacgatgc gcccattgcc
     2941 aatgcggaga tcacgctagc ttgctccgat cctcttcctt tcgttgcaaa tacagatgat
     3001 acgggccacg tcacgattcg ccgtctctgc ccgccgccct acgtgttgaa agcaaccgca
     3061 cctggctttg aatccacgtc gcgaacgaat gtgatgccga attccccggc gacccgtgtt
     3121 tccttacgaa agcttggttg gatcgaagtc aatgtgatcg atcggaacaa gaagcccgcg
     3181 ccgttatcca cgatcttcgt ggcgggtcca gcgatttggc cggcgcgaca agtccaaagc
     3241 gatgcttctg gctatagcaa aatcagtgcg cttcccgctg gcgcttacga tcttcgcgct
     3301 acgcgccaaa gccttgtttc tcccatttcc acgggcatca tggtcgaacg cggcaaagga
     3361 acacccgtaa cgctcacgct cgatgaaggc cgcatggtca ccgtatcggt cacaggcggc
     3421 ttggccgacg acgctcccgc tgtcgccagc gcaaacgtcg tactcgtgga gggaggattg
     3481 tcttcgttcc cattgcaagg tcgcacgggc ctcgacggga ccgttcagct tggacctttt
     3541 cctccggtgc ctctcagtgc ttctgcacga gcggaaggat tcgtcagtcc gggtgtcgta
     3601 ttggtcgggg ccaacgatac gcaagtgcgt atcgctttgc tgaaagcagg gaaactaacc
     3661 ggagaagtcg tggataatcg cggatttccc gtgccgggct gctcgatcga agtcatcggc
     3721 accgacttgg ccggcttgcc catcgccgaa acccccgccg cgatggcgtt tcgtcgcgcc
     3781 catttcgcat gggccatcca cggccctccc acgttgattc cagcagggga actcggcgtc
     3841 atgcccggac ccattccccc gatcccaaga ggcgatatca gcttggattc ccgccttcct
     3901 tcgttcgagg agaaccgcga ttcgcagcaa ctcaccccct gggtcacaga tggtcacgga
     3961 cgcttttccg tgtcaccggt tccgccagga cgcatcagcg ccatcgtgcg tcatccggcc
     4021 catgtcgaag ccatttcgga agccgtgacg cttgcgccag gaggtaccgc tcacgttcgt
     4081 gtcgtccttc atcttggagg caccctcgaa ggggtcgtgc tcgacgatcg ccgcttcccc
     4141 atctctggcg catgggttcg tgtcaccgca aaagaaggtt cgtccgagcg cacttccatg
     4201 accgccatgg atggttcttt tgcgttcacc gcggtggcac ccgtcgtcat cgtaacggca
     4261 tcgcgccctg aatctcccga caaggtggct tatcaagaaa cgctatcggt ggcggccgaa
     4321 gagcgaaaac aggtggaaat cgttctggcg cgcgaaaggc cagccatgac cgtccgcgtg
     4381 accgacgacc gtggttatcc tatcgatcga gttcatatcc gcgcggtatc cctcacgacg
     4441 gatgtgtcgc tgcgtcgcac atcgttcagc gacagcaaag gcaatgcaat catcgaggaa
     4501 gccgcgggaa ttcgacttcg gctcgaggta tccgcaaacg gatacgggac acacgtcgag
     4561 accttcgata cctcacccga gatggtggag gtcaggctcg atcgaggatt gcgcgtgcaa
     4621 ggcgaggtga cgtcgagagc agggcgagac cgggttcaag gcgccacgat tacagcttat
     4681 cttcccacgg gaacccgaca ggtgacgacc aacgaaaacg ggatgttccg tctcgacgat
     4741 ctgtcatcag ggccgttacg gatccgggtg gatcatgaaa gctatgtatc gctggataaa
     4801 acttttgagg tgaatactcc gagtgatgtg gagcgaccgg ttcaactcga gccgatggcg
     4861 ttggaattgg cgggaatcgt ggaaggcgag gtggtggatg accgtggcaa tcccgtggct
     4921 ggagcgcggg tgtcgaaaga ccaggtgccg gaatatttgc ctgtgggacc gcttccccca
     4981 ggagtggtgg tcaccgatga gaaagggaaa tttcgtttgg gtgggcttgc ggagggtgag
     5041 ataacgttgg aagcgtttgc tcctggaatt ggccggggta aggaagaacg cattgccgtt
     5101 cgcaaggagc gaacgaccac gagagtgcga atccgtctcg accctacccg aagcaaagca
     5161 gggcctgcga tcgtggccgg tgtagcagtg accttagcgc cttccacggt tggcccaggg
     5221 gctctcgtca cgggggtagc aagcggaagc gaggccgatc gcgcgggtat tcggatcggg
     5281 gactatatcg aacggatcga tggggtcgta cccgacgacg agcgggatgc catgcaacga
     5341 ttgcgtggac cggagcggca agatgtgatt gtggatattc aacgtgaaaa caagcatttg
     5401 acgatccgcc tccccaggga gcgtgcaaga caataaccac gacccatggc cgatctttcg
     5461 tccgacaaga tgcacgatgc ttgttttctc aggttgagcc cagttgtggg tctggagcgc
     5521 gcgcatgagg caacgctggg ctcgaggtgt acccctggct gtcgaggctc ttcgtgatcg
     5581 cctgctcctc agtaatcttg tggttgcgac ggcccgtgtg gaaagtcccg agcaagtcaa
     5641 gaatcacgat ttgcaccgat gaaaagaaga tcaaaccgat gacgacagga gccatgcccc
     5701 cacggaaaga atcccaatca atgagcttgt atatcaggta gccaaaccca gcgagtgcgc
     5761 tgagcatggc gacgacaaac cccgtacacg aggtcaaaca tagtttttga attgcataca
     5821 tgagcggcgc ttctttcgag ccttttttta cggctgcgac aatgcggtag ccgttatccc
     5881 gttcgcggat gaagtctgcg atgagttccg gggggcttga aggtcggcgc attggcaggc
     5941 gatggcgtcg ccttgaactt ggaacaaggc gtgcgggggg gagcgggtga tacggaaatt
     6001 gcgtgtattg gcgatgactt tgatgcgatg gtcggtagtg gacggttctt tcaggacttt
     6061 ttacggtgcg gtctgtggag gcgttatcga tgaagatgcg ctcccagcgg taggtggggc
     6121 cgaggctttc gagggtttcg acggttcttt ggtgaagggt tcggatattg tattcttcgt
     6181 tgaagcatgg ggtgacgatg ctgatgagtt tcgttgtcac ggggcgtagg gctgagccat
     6241 cccctcctat tatcccgagt atttccctcg ttaaacgatg ttcctgaaga acctcggcat
     6301 aagccgtaac caaccgctcg agtcgttcc
//
LOCUS       DAOSOR010000330         6327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_12416_length_6327_cov_5.131059, whole genome shotgun sequence.
ACCESSION   DAOSOR010000330 DAOSOR010000000
VERSION     DAOSOR010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12416_length_6327_cov_5.131059"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(413..1159)
                     /locus_tag="PK710_16275"
     CDS             complement(413..1159)
                     /locus_tag="PK710_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11339.1"
                     /translation="MVRRICWAMTACFLFLGCVPVQEPYRPYCSGGKRLVDGHCVCAL
                     GMTWNGKFCQGEPQAGHCMNGSVPYGRECQCPDGTTLDDTGHCIALECESRNAIVSGN
                     ECVCAEGTEWKADQGRCVEFSCLGGSVVSGDQCVCVNGQVWMDGRCATPEYDTEPEER
                     RTSRRRHHRSNHGHADRRGSSSCNAYGCHEEGGGCNAYGCWEAGGGCSAYGCWKHRGG
                     CNAYGCWRAPGACNAYGCSNRGECNAYGCP"
     gene            complement(1235..2551)
                     /locus_tag="PK710_16280"
     CDS             complement(1235..2551)
                     /locus_tag="PK710_16280"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOT11340.1"
                     /translation="MRISVFGSGYVGLVTAAGLADMGHRILCVDIDESRIKRLRAGQV
                     PFFEPGLADLVARNLRGERLRFATELDEEFASADAHFVAVGTPPLADGRADVSAVFAV
                     GHTLAQRVTQEGIVAVKSTVPVGTCDALQRLLDDSCPHRMHVASNPEFLKEGAAVDDF
                     FRPHRVIIGVDDPKTGEAMREMYRPLQLSRERVIVMSRRSSELTKYAANSMLATRISF
                     MNELSRLCDAIGADIHAVRAGIGSDERIGRAFLYAGPGYGGSCFPKDVSALIHLGKEA
                     GVTLGIVEAVAEANEAQRLYVWEKIRSLFADGLQGKTVTVWGLAFKPMTDDVRDAPAG
                     VIVTKLLEAGAIVRAHDPEAADNFAAAYAPANDAISYHVNEYEALDGADALVLMTEWR
                     HFRNPDFGEIKRRMRGRGIVDARGIWTMFGLSQAGFDYRTIGSPAA"
     gene            complement(2561..2842)
                     /gene="secG"
                     /locus_tag="PK710_16285"
     CDS             complement(2561..2842)
                     /gene="secG"
                     /locus_tag="PK710_16285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOT11341.1"
                     /translation="MFETILSIIHVFACFFLILVVLLQQGKGGGLGAAFGASTTRQVF
                     GGRGAGNLLTRLTAVTAAVFMTTSVVLAYIASSGDRSLRATEAEVEKMP"
     gene            complement(3154..3921)
                     /gene="tpiA"
                     /locus_tag="PK710_16290"
     CDS             complement(3154..3921)
                     /gene="tpiA"
                     /locus_tag="PK710_16290"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOT11342.1"
                     /translation="MNKARRPLIAGNWKMNHGGANACNLASAVKRTTAEFDKVDVVVC
                     PPFTAIAWVAEELRGSAVQVGAQNVHPKDSGAFTGEVSASMLLDAGARWVILGHSERR
                     AMFGETDTLVAEKTRVALDVGLRPIVCVGELLEEREKGQTLDVVKRQLNAFASILADK
                     PGVGAIAYEPVWAIGTGKTAGPAEAQEVHAMIRARLREISAEVAEKTRILYGGSMKAS
                     NAEGLLACEDVDGGLIGGASLKAEDFAGIALAGQKLA"
     gene            complement(3955..5166)
                     /locus_tag="PK710_16295"
     CDS             complement(3955..5166)
                     /locus_tag="PK710_16295"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOT11343.1"
                     /translation="MMSLAGIKTIKDLDLENQRLFLRVDFNVPLDKKTGEITDDARIR
                     AALPTIEYAMEAGARVILASHLGRPKGERVPSMSLEPVGARLAELLNVDVLFPEDCIG
                     DAPKKLINEMRGKQIVLLENVRFHAEETQNDEAFARQLLELCDAYVNDAFGSSHRAHA
                     SVHALPRLLQNRACGFLLQAEIEKLGKIVTNPDKPYLAVLGGAKVSDKIAVVESLLNL
                     VDALVIGGAMANTFLKALGKDTKASKVEEDKLALARTIVEKAKDKNVDLILPEDVVVA
                     ADINATQGITVDAGAIPDGQMALDIGPKTVARIEAVCRSSKSIFWNGPMGMFENPAFA
                     SGTFAVARAMAATKAFTVVGGGDSASAVRKAGDDIASAFTHISTGGGASLELVEGRKL
                     PGIEVLRFIEA"
     gene            complement(5253..>6327)
                     /gene="gap"
                     /locus_tag="PK710_16300"
     CDS             complement(5253..>6327)
                     /gene="gap"
                     /locus_tag="PK710_16300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOT11344.1"
                     /translation="PAIDACEVGERIFGGNLMATKIGINGFGRIGRCVLRAIVERGLD
                     DIEVALINDITDDTTLAHLLKYDSTFGVFKHDVSVKSGAIEVAGRTIKTSEVRDPKEI
                     AWKAEGVDVVLECTGLFVSADKAKWHLDAGAKKVLLSAPGKGEGIRTIVLGVNDKDYD
                     PKTDHIVSVGSCTTNCLAPVARVLLDNFGIKYGLMTTTHAYTNDQNLLDLPHRKGDLR
                     RARAAAVNMVPTTTGAARAVALVIPELKGKFDGLAIRVPTIDVSLVDLVFQSERPMTK
                     ESINEAMKKAAQTPQLKGKLQYNDLLLVSSDMIGNPHSSIFDATMTTVISENMAKVFS
                     WYDNEWGFSNRMVDFLQILAASK"
BASE COUNT         1432 a         1970 c         1629 g         1296 t
ORIGIN      
        1 ggcagggaat gtgccgacga gccccaatgc tgctccggct attgttccgg ctccccaaaa
       61 aaatgctgca tcacgaccgg ggacaaatgt tcgaaacccg aggaatgctg ctccggcgca
      121 tgtaacggaa aatgctgtcg gggcgtacgc aaggcctgcg aagagcatgc cgattgctgc
      181 tccggctatt gttccgattc ccaaaaaaca tgctgcatca cgaccgggga caaatgttcg
      241 aaacccgagg aatgctgctt caatcgttgt gaaagcggga agtgttgtcg tcccaaaggc
      301 aaggagtgta attccccttc ccagtgctgc tccaagacat gcacgaacaa gaaatgtaat
      361 taatttataa taaattcaat atattacaca cattttcccc aagcatcgat tcctagggac
      421 agccatacgc attgcattct ccccgattcg aacacccata cgcattgcaa gctcccggtg
      481 cccgccaaca cccgtacgca ttgcagcccc cccggtgttt ccagcaccca taggcgctgc
      541 accctccgcc cgcttcccaa cacccatagg cattgcatcc ccctccctct tcatgacacc
      601 catacgcatt gcaactcgaa gacccccgac gatccgcgtg accgtggttc gaacgatgat
      661 gccgtcgcct cgaagtccga cgttcctccg gttccgtatc gtattcagga gttgcacaac
      721 ggccgtccat ccacacctga ccattcacac aaacgcattg atccccggag accaccgatc
      781 ctcccaaaca agagaactcc acgcatcgcc cctgatccgc tttccattcc gtcccctccg
      841 cgcatacgca ttcattgccg gagacaatcg cgtttctcga ctcacattcg agagcaatac
      901 aatgacccgt gtcgtccagg gtcgtcccat caggacattg acattctcgc ccgtatggca
      961 cgctgccgtt catgcaatgg cccgcctgcg gctctccctg gcaaaactta ccgttccacg
     1021 tcattccaag cgcgcaaacg cagtggccgt ccaccagacg tttccccccc gaacaatacg
     1081 gacgatacgg ctcctgaacc ggaacgcacc caagaaatag aaagcaagcg gtcatcgccc
     1141 agcatattcg tcgaaccatg acgatcctcc aacgtcatga gacttcaaac ggcgcaagtc
     1201 accggtcccg tcgcagcgct aacaaatatc gaacctatgc cgcgggagaa ccgatggttc
     1261 gataatcgaa tccggcttgc gaaagcccaa acatcgtcca gatcccccgc gcgtccacaa
     1321 ttcccctgcc tctcattcgc cgcttgattt caccgaaatc gggattgcgg aagtgacgcc
     1381 actcggtcat gagcacaagg gcatcggctc catcgagcgc ttcatattcg ttgacgtgat
     1441 agctgatagc gtcgtttgcg ggagcgtaag ccgcggcgaa gttgtccgcg gcttccggat
     1501 catgcgccct gacgatggcc ccggcttcga gcaatttcgt cacgatgaca ccagcaggcg
     1561 cgtcgcgcac gtcatcggtc atgggtttga atgccaatcc ccacaccgtc acggtcttgc
     1621 cctgcaaacc atccgcaaat agggagcgaa ttttttccca tacgtaaagg cgttgggcct
     1681 cgttggcttc ggccaccgcc tcgacgatac caagggtgac gccggcttct tttcccaagt
     1741 ggatcaacgc ggaaacatct ttcggaaaac acgatccacc atatcctgga ccagcgtaca
     1801 aaaaggcgcg gcctatgcgt tcatcgctac cgatacccgc gcgaaccgca tgaatatccg
     1861 caccgatggc atcacagagc cgggacaatt cattcatgaa cgagatgcgc gtggcgagca
     1921 tcgagttggc ggcatacttg gtcaactcgc tggagcgacg gctcatgacg atcacccgtt
     1981 cgcgggacag ttgcaacggg cgatacatct cgcgcatggc ttccccggtt ttgggatcat
     2041 ccacgccgat gatcacacgg tgaggtcgga aaaaatcatc gactgccgca ccttctttga
     2101 gaaactccgg attggaggcg acatgcatgc gatgaggaca agaatcatcc agcagtcgtt
     2161 gcaaagcatc acaagtgccc accggaactg tcgatttcac cgcgacaatc ccttcttggg
     2221 tcacccgctg cgcgagggta tgcccaaccg caaaaacagc ggagacatcg gcgcgaccat
     2281 ccgcgagagg gggcgtgccc accgcaacga aatgagcgtc tgccgacgcg aactcctcat
     2341 cgagttcggt ggcgaaacgc aatcgttctc cgcgtaaatt gcgagccacg agatccgcga
     2401 gccccggttc gaaaaacggc acctgtccgg cgcgaaggcg ttttatccgc gactcatcga
     2461 tgtccacgca caggatgcga tgccccatat ccgcgaggcc cgcggccgtc accaggccca
     2521 cgtacccgct tccgaacacg gaaattcgca tggcagctcc tcagggcatc ttttccactt
     2581 ccgcttccgt ggcgcgcaag ctccggtcac cgctcgatgc gatatacgcc agcacgacac
     2641 tggtggtcat gaacaccgcc gccgtcacgg cagtcaatcg cgtcagcaaa ttgccagcac
     2701 cacggccacc aaatacctgg cgagtcgtcg atgcgccaaa agccgcacca agaccgccac
     2761 ccttgccttg ttgcaaaagc acgacgagga tcaaaaaaaa gcaggcgaag acgtggatga
     2821 tgctcaggat cgtctcaaac atgactgtgc ggggttctac gacaccagaa ggtgttgtgc
     2881 cataaggatc gcttcgaggc gcggatcatg aaacggtttt gacccgaacc cgatggtctg
     2941 cgctccattt cccttaggaa agaagatatg tcaaggatgg cgatgggtgg atgagatacc
     3001 gcgtcatccg ccgaaaaatg tagggagggg gcgggggtca gccaggggaa tcctcattaa
     3061 aggattatat ttcgaatggt tacacacaac cgggcgaagt aggcgcaacc cgatgcctaa
     3121 cccggcgtgc cgaaaagagt gaattcgtgg gggttacgcc agtttttgtc ccgccagggc
     3181 aattcctgca aaatcctctg ctttgaggga agcaccgcca atcaagccac catccacgtc
     3241 ctcacaggca agtaaccctt ccgcgttgct cgccttcatg ctcccaccat acagaattct
     3301 cgttttttcg gcgacttcgg ctgagatctc gcgcaggcgc gcacggatca tcgcatgcac
     3361 ttcttgcgct tccgcaggtc cggcggtctt gcctgtgcca atcgcccaca cgggctcgta
     3421 agcaatcgct cccacgcctg gtttatccgc caaaatcgat gcaaaagcat tgagctgacg
     3481 tttgacgaca tcgagcgttt gtcccttctc ccgctcttcg agcagttcac ccacacaaac
     3541 gatgggtcga agccccacat cgagggccac acgagttttc tcggctacca gcgtatcggt
     3601 ttcaccaaac atcgcacgac gctcgctatg gcccaggatt acccatcgag ccccggcgtc
     3661 gagcagcatg ctggcactga cttctcccgt gaaagcgccg ctgtctttgg gatggacgtt
     3721 ctgtgcgccg acctgcacgg cactgcctcg gagttcctcg gcaacccaag ctatcgcggt
     3781 aaatggagga caaaccacca catcgacctt atcgaactca gcggtggttc gttttaccgc
     3841 ggaagcaaga ttgcaagcat tggctccgcc gtgattcatc ttccaattgc ccgcgatgag
     3901 cggacgtcgt gccttgttca tcagctatct ccgaaatggg aaaaggaggg gacgctacgc
     3961 ttcgatgaat cgaaggactt cgattccggg aagttttcgt ccttccacga gttcgaggga
     4021 agcgccgccg ccagtcgaga tatgagtaaa cgcgctggcg atatcgtcgc ccgctttgcg
     4081 aacagccgaa gcgctatcgc caccgccaac gaccgtgaac gctttggtag cggccatggc
     4141 acgggcgacg gcgaacgttc cggaagcaaa ggccgggttt tcgaacatgc ccatcgggcc
     4201 gttccagaaa atgctcttgg acgatcggca taccgcctcg atgcgagcca ccgttttggg
     4261 gccgatgtcg agcgccatct gaccgtcggg aatggcaccc gcatccacgg taataccttg
     4321 cgtggcattg atatccgccg cgaccacaac gtcctcgggc aagatgagat ccacgttttt
     4381 gtctttggct ttttcaacga tggtgcgggc aagggcgagc ttgtcttcct cgactttgct
     4441 ggccttcgtg tccttgccaa gcgctttgag aaacgtgttg gccatggcgc cgccgatgac
     4501 caaagcatcg accaggttga gcaacgactc gaccaccgcg atcttgtcac tcaccttcgc
     4561 gccacccaac acggccaaat acggtttgtc cgggttggtc acgatctttc ccagcttctc
     4621 gatctcagct tgcagcaaaa aaccgcaagc acgattctgc agcaaacgag gcaacgcatg
     4681 gaccgatgcg tgcgcccgat gcgaagagcc aaaagcatcg ttgacatacg catcacaaag
     4741 ctcgaggagc tgccgcgcga aagcctcgtc gttctgtgtc tcttccgcgt gaaaacgaac
     4801 gttttccaac agaacgatct gcttgccgcg catctcgttg atcagctttt tgggcgcatc
     4861 accgatgcaa tcctcaggga ataggacatc gacattgagc agttcggcaa gccgtgcgcc
     4921 taccggttcg agggacatcg acgggacgcg ttctcctttt ggacgcccca agtgagaggc
     4981 gaggatgacc cgggcacccg cttccatcgc atattcgatg gtaggaagag ccgcgcgaat
     5041 ccgcgcgtcg tccgtaatct ctcccgtctt tttgtcgagc ggcacgttga agtcgacccg
     5101 taggaatagc cgctgattct cgaggtcgag atccttgatg gtcttgattc cagccaaact
     5161 catcatgcct acacctccgc ggaggaacaa aggggcgcaa tgccgaaagc accgtgggca
     5221 gaagacccac ggcgccacgg caccgcttca actcacttcg atgcagcaag gatctggagg
     5281 aagtccacca tgcggttcga aaacccccat tcgttgtcat accagctaaa taccttcgcc
     5341 atattttcgc taatgacggt ggtcatcgtg gcatcgaata tggaggagtg cggattgccg
     5401 atcatgtccg agctaaccaa caacaggtcg ttgtactgga gctttccttt gagttgaggc
     5461 gtttgggcgg ccttcttcat cgcctcgttg atgctttcct tcgtcatcgg acgttcactc
     5521 tggaaaacca ggtccaccaa cgatacgtcg atggtcggca cacgaatcgc cagtccatcg
     5581 aatttgcctt tgagttcggg aatgaccagc gccaccgcac gagccgcgcc cgtggtggtg
     5641 ggaaccatgt tgacggcggc cgcgcgcgca cgacgcaaat cgcctttgcg atgcggtaga
     5701 tccaacaagt tctgatcgtt ggtgtaggcg tgggtcgttg tcatcaaacc gtacttgatg
     5761 ccgaagttgt ccaacaacac cctcgcgacg ggagcgagac aattggtggt gcagcttcct
     5821 accgatacga tgtgatcggt cttcgggtca tagtccttgt cgttgacgcc cagcacgatc
     5881 gtgcgaatgc cctcgccttt tcccggcgca ctgagcaaaa cctttttcgc ccccgcatcc
     5941 aaatgccact tggccttgtc ggcggaaacg aaaagacccg tacactccag aacgacatcg
     6001 actccctcgg ctttccaggc aatctctttc ggatcccgga cttcagacgt cttgatcgtt
     6061 cgccccgcga cctcgatagc accagacttc accgatacat cgtgcttgaa cacgccgaac
     6121 gtcgagtcgt acttcaacaa atgcgccagc gtggtgtcgt ccgtaatgtc gttgatgaga
     6181 gccacttcga tgtcgtcgag gccacgctcc acaatcgcac gcaacacgca acgaccaata
     6241 cggccaaatc cgttgatacc aattttcgta gccatgaggt ttcctccaaa aatacgttcc
     6301 cccacctcgc acgcgtcgat agcgggc
//
LOCUS       DAOSOR010000331         6320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_12434_length_6320_cov_4.700878, whole genome shotgun sequence.
ACCESSION   DAOSOR010000331 DAOSOR010000000
VERSION     DAOSOR010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12434_length_6320_cov_4.700878"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PK710_16305"
     CDS             <1..329
                     /locus_tag="PK710_16305"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT11345.1"
                     /translation="RRIRRARAERGMTQSDLAHGARVGANYIPRLERGELVPSVEAAF
                     RIARALGLSLDEMCGRTPVRKDGPDLQDALGRLEKTDPASLRRVADVIEALSAPSGRR
                     AGRKTK"
     gene            615..1412
                     /locus_tag="PK710_16310"
     CDS             615..1412
                     /locus_tag="PK710_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11346.1"
                     /translation="MDFHALCRSALVIGFTVGLGACATGETASGYAGGNGGGGESGNG
                     GTNSDSGDVGGGTGGSGASGGASGTGGAGGSGASGGASGTGGASGTGGAGGTGGAGGS
                     GGDCLSCGAPVAACTSTTTSRKGCDQAQIVGRNEFVGGASYTANGVTLTGGQYTSGCG
                     NNAGSDNAYRLYLWAGEELSVTMSPTKGGFTPRIGWYFVGASWTVPPGSCGTKLSCAD
                     ATGSAPFTSSHSAAQTGWYVVVVGSNSYTKNDGEYNMSMSLQCSCGC"
     gene            1426..2637
                     /locus_tag="PK710_16315"
     CDS             1426..2637
                     /locus_tag="PK710_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOT11347.1"
                     /translation="MRFYYAFRWVPITIGFTLAACSASGDSNSKGLTSGTGGSPSTTD
                     GSIADTTSVEDSTVHLDVTSSNDGPLPEGDVCVGTKSNAQPTPLNVMVMLDQSSSMKG
                     ARWTSVVNAIKSFTQAPEAAGIRIALNYFALTAPCNTNADCKSVLGSCGSTKFCTFDD
                     YCDASAYETPEVEFAELPGAAGAIAASLDAHAPLFNTPTPPALLGAINHAKAWAIAHP
                     SETVVVVLATDGEPDSVTCKIGSGDVSKVVAIAQGGLQATPSIPTYVIGVGNHLSSLN
                     SVAEAGGTTKAYIVDGGGGNATQKQFLDAMNAIRGSALPCSYVIPDPPSGTLDYGKVN
                     VLYTSANGTTSTIGQVPSAADCDASGGWYYDDPVHPNVIVVCPATCELFHTGSAGSVE
                     IQLGCKTVQVK"
     gene            2718..3401
                     /locus_tag="PK710_16320"
     CDS             2718..3401
                     /locus_tag="PK710_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT11348.1"
                     /translation="MTTILVVDDEPRIRDIVQYTLSKEGFEVLLASNGNEALGLLKSK
                     DIDLVVLDVLMPDLDGLSVCRTLRTFSDVPCIFLSSRGEEVDRILGLELGGDDYLTKP
                     FSPRELATRIRTVLRRTARTESNKETLVHGPIELDLVRHELRVKGDRINLTVTEFAVL
                     QALLERPGRLLTRSQLIDRAYIHDNHITERTMDTHIRRIRAKLRPLGVDPIETVHGLG
                     YKAGEIENP"
     gene            3401..4903
                     /locus_tag="PK710_16325"
     CDS             3401..4903
                     /locus_tag="PK710_16325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT11349.1"
                     /translation="MGKRFARWVDELFRIRTRLLLVHVLIVAVPLLGIFFARFYEREM
                     LRALEQNMIDQAEVLRWMLANDPHGVNRQGLAQDLARSARETRTRIRVLDPQGQLVAD
                     SHEQGPPEGPEEPLPSLQPGQPRYETAAMSPPEPLDVSQRTEIQAALQGKYGSNTRFW
                     ENRDRLYLFSALPIVRDGSVQGVVYVTRSTNPVRSAMYRLRSSLFIILVAALAATSVL
                     SMFLAGTISRPLTRLTHIANRIAKGDRSQTLKMARRDEIGQLAHAFDTMTRKLDDRAK
                     YVGQLAANISHEFKSPLTGIRGAAELLRDGAAEEPEARIRFLGNILADANRLDRLVTR
                     LLELARFEADPIPDEMIDYEELVQEVARQAAATVPVSVQFHTHHPLVFGKRFHLVSAL
                     SNLVDNAQQHAAEGTEVSIVVEDGPEERLSTTVHNRGEPIHAINLPRIWDRFFTTRTE
                     KGGTGLGLPIVATVIEAHGGSVSVRSHAAEGTIFRFDLPTVGVGRRAEAR"
     gene            complement(4906..5796)
                     /locus_tag="PK710_16330"
     CDS             complement(4906..5796)
                     /locus_tag="PK710_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT11350.1"
                     /translation="MPVTLLAVDDSVTMRKVYEMTFGGEDFRVLTADSAETALALFRS
                     EKPSLVLADITLPDKNGYELCASLKQAQPELPVLLMSSKLNPYDKARGQAANADDNVD
                     KPFDSQKLIERVNSLLSGQKTVASPSAAKPAATAPRASVPQGATLLGQGPPRPQVTRP
                     APIAAPTANQPVNLRATADFAVRPAAGPSARAPLPPSTTTMGTPPPAVVQAKPVEAAW
                     PAKPSAEPAKEPVKPVMAQSSKADGDLSAKLQALGLTKDQMEGVLALSHELVERVVWE
                     VVPVLAETIIKEELKRLTED"
     gene            6029..>6320
                     /locus_tag="PK710_16335"
     CDS             6029..>6320
                     /locus_tag="PK710_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11351.1"
                     /translation="MTYHVKTCFRTIQGEGAQTGRVAVFCRLSGCNLWSGRIDDRDRA
                     ICKFCDTDFVGTDGPAGGSFATATDLAEHIAVIWQQQTVQDEHRYVVFTGGES"
BASE COUNT         1355 a         1731 c         1901 g         1333 t
ORIGIN      
        1 gtaggcggat ccggcgcgcg cgtgcggaac gcggcatgac gcaatccgac ctcgctcatg
       61 gagccagagt tggggcgaac tacatccctc gcttggaacg aggcgaattg gtgccatctg
      121 tcgaggcagc ctttcgcatc gcaagagcgt taggactctc gctcgatgaa atgtgcggcc
      181 ggactcccgt gcgtaaggat ggccccgatt tgcaagacgc cttgggacga ttggagaaaa
      241 cggatccggc atccctgcga cgcgtggccg atgtgatcga agccttgagc gctccatctg
      301 gacgacgagc cggtcgcaaa acgaagtgac cccatgattc ctcacggggg tcatcaagac
      361 ccatgggtgc gtgttccacc cacgcgtggg cggactgctc cgaactgcaa tcacaccgga
      421 tctccctgat gattgtcccc agcgaaccgt agctccccca cggtctcgcg ttggagttgc
      481 gccaatttcc tctccggata tacttggaaa tccaggggta ctggtgtttg gtcgttgttt
      541 cgcggagatg cgatgggtgc atgagcacgt tgcttccatc tgtttgggga cccattaaaa
      601 tctggagtaa tatcatggac ttccacgctc tttgccgcag cgcgctggtc atcggattca
      661 cggttggact tggggcttgt gcaaccggcg aaaccgccag tggttatgca ggcggaaatg
      721 gcggtggcgg tgaaagcgga aatggaggca caaacagcga cagcggcgac gtagggggag
      781 gcacaggcgg aagtggagca agcggtggcg cgagtggaac cggtggcgcg ggcggaagtg
      841 gagcaagcgg tggcgcgagt ggaaccggtg gcgcgagtgg aaccggtggc gcgggcggaa
      901 ccggtggcgc gggcggaagt ggtggagatt gcctgagttg cggcgcgccg gtggcggcat
      961 gcacttccac caccacgagc cgaaagggct gtgatcaagc ccaaatcgtg ggtcgaaacg
     1021 aatttgtggg cggcgcttcg tacacagcca atggtgtgac gttgacggga ggacaataca
     1081 cttcgggctg tggaaacaat gccggcagcg ataacgccta ccgtctttat ctttgggcgg
     1141 gggaagaact gtccgtcacc atgtccccca cgaaaggggg attcacaccg cgcatcgggt
     1201 ggtacttcgt tggcgcgtca tggactgttc ctcccggcag ttgcggaacg aaactatcct
     1261 gcgccgacgc cacgggctcc gcaccgttca caagcagcca ttccgcagca caaaccggtt
     1321 ggtatgtcgt cgtcgttggc tcgaacagct acactaagaa cgatggtgag tacaacatga
     1381 gcatgtcgct ccaatgctcc tgcggctgtt gaaatggagg taattatgcg tttttattat
     1441 gctttccgtt gggttccgat cacgatcggt ttcaccctgg ccgcttgcag cgcttcgggc
     1501 gattcgaatt caaaagggtt gacgagtggc acgggaggct cgccctctac gaccgatggc
     1561 tcgatcgcgg acacaaccag cgtggaggat tcgaccgtcc atctcgatgt cacctcctcc
     1621 aacgacggtc cgcttccgga gggtgatgtt tgcgtcggca cgaaaagcaa cgcacagcca
     1681 acacctctca atgtgatggt catgctcgac caatcatcgt cgatgaaagg agcgcgctgg
     1741 accagcgttg tcaacgcgat caaaagcttt acgcaagcgc cggaggcagc ggggatccga
     1801 atcgctttga attattttgc gttgacggct ccctgcaaca cgaatgcgga ctgcaagagc
     1861 gtgcttggtt cgtgtggttc cacgaaattc tgtacgtttg atgattactg cgatgcgagt
     1921 gcgtacgaga cgcccgaggt tgaatttgcc gaattgcctg gcgccgcggg tgcaattgcc
     1981 gcttcgttgg atgcacatgc tcccttgttc aacaccccaa caccacccgc attgcttgga
     2041 gcgatcaatc acgccaaagc atgggccatc gcgcacccaa gcgaaaccgt ggtggtggta
     2101 ttggccaccg acggtgaacc cgacagcgtg acgtgcaaaa tcggtagtgg tgacgtatcc
     2161 aaagtcgttg ccattgccca agggggtctt caagcaaccc catcgatccc cacctatgtc
     2221 atcggcgtag gcaatcattt gagcagcctc aattccgtcg ccgaggcggg aggaaccacg
     2281 aaagcttata tcgtggacgg tggagggggc aacgcaaccc aaaagcaatt cctcgatgcg
     2341 atgaacgcga ttcgcggctc ggcccttccc tgcagctatg tcattccaga cccaccaagc
     2401 ggcaccctcg actacggaaa agtcaacgta ttgtatacgt cggcgaacgg cacgacatct
     2461 acgataggtc aagtaccaag cgctgcggac tgcgacgcat cgggaggttg gtactacgat
     2521 gaccctgtcc atcccaatgt catcgtcgtt tgcccggcca cctgtgagct attccacacc
     2581 ggttccgcag gaagtgtcga gatacagctc ggatgcaaga cggtccaagt gaagtaatcg
     2641 ttcgatgggt tcttgttgtt ccgacgatcc ccatcgatca tcatttcgtg gacccagcag
     2701 attcgcggga gagtgccatg accaccatcc tggtcgttga tgacgaacca cgcattcgcg
     2761 acatcgtgca atacacgctg tccaaagaag gtttcgaagt gctgcttgca tccaatggca
     2821 atgaagcgct cggtttgttg aagagcaagg acatcgacct ggtggtgctc gatgtgctga
     2881 tgccggacct cgatggtctg agcgtgtgtc gaacccttcg caccttcagt gacgtgcctt
     2941 gtatctttct gtcttcgcgc ggggaggagg tcgatcgaat ccttggattg gaactaggag
     3001 gcgatgacta tctgacgaaa ccgttttcgc cccgggaact tgccacacgc attcgtacag
     3061 tgttgcggcg cactgcgaga accgaaagca acaaggaaac cttggtgcat gggccaatcg
     3121 aactcgatct cgtacggcac gaactgcggg tgaagggcga tagaatcaac ttgaccgtca
     3181 ccgaatttgc cgtgcttcag gcattgctcg aacgtccggg tcgcttgctg acacgctccc
     3241 agctcattga tagggcctat attcacgaca atcacatcac cgagcgaacg atggatacgc
     3301 atatccgtcg cattcgtgcc aaattgcgcc cccttggtgt cgatccgatc gagacggtcc
     3361 atgggttggg ttacaaagca ggcgagattg agaacccgtg atgggaaagc gattcgctcg
     3421 atgggtggat gagctattcc gcattcgaac gcgtcttctg ctggtgcatg tattgatcgt
     3481 cgccgtacct ctgctcggca ttttcttcgc gagattctac gagcgagaga tgctccgcgc
     3541 tctcgagcaa aacatgatcg atcaagccga ggtgttgcga tggatgttgg caaacgaccc
     3601 gcatggagtg aatagacaag gacttgccca ggaccttgcg cgctcggcac gcgaaacacg
     3661 cacgcgaatc cgcgttcttg atccacaggg gcagcttgtg gcggactccc atgagcaggg
     3721 accaccggaa ggacccgaag agccgttacc gtcgctccag cccggtcagc cacgatacga
     3781 gacggcggcg atgagtcccc cagaaccgct cgatgtgtca caacgcaccg agattcaggc
     3841 cgccctacag ggaaaatatg gttccaatac gcggttttgg gaaaaccgag atcggctgta
     3901 tctcttcagc gccttgccca tcgtacgcga cggttcggtg caaggcgtgg tctatgtcac
     3961 acgctcgacc aatcccgtgc gaagcgcgat gtatcgatta cgttcatccc tgttcatcat
     4021 tctggtggcg gcgctcgcgg cgacgtccgt attatcgatg tttctcgccg gaacgatctc
     4081 ccggcccttg acccgcttga cccacattgc caatcgcatc gccaaaggag atcgaagcca
     4141 aactttgaaa atggcgcgtc gcgatgagat cggacagttg gctcatgcct ttgacacgat
     4201 gacacgcaag ctcgacgatc gagcgaagta cgtgggtcag cttgcagcca acatcagtca
     4261 tgaattcaaa tcaccgctca cgggaattcg tggagccgcg gaacttctgc gggatggggc
     4321 ggcggaagaa ccggaagcgc gcatacgctt tctcggcaat attttggcag atgccaaccg
     4381 tctcgatcga ctggtgaccc gtttgctgga actcgctcga ttcgaggcgg atccgattcc
     4441 tgatgagatg attgattacg aagaattggt gcaagaggta gctcgacaag ccgcggccac
     4501 tgtgccggtt tccgttcagt tccacacgca tcatccgctc gtatttggga aacgatttca
     4561 tctcgtctct gcgttgagca atctcgtgga caacgctcaa caacacgctg cggagggaac
     4621 agaggtaagt atcgttgtgg aggatggacc ggaggagcga ttatccacga ccgtacataa
     4681 cagaggagaa cccatccacg cgatcaatct gccgagaata tgggatcgat tttttacgac
     4741 acgcactgaa aaagggggaa cgggattggg ccttcccatt gtcgcgacgg tgatcgaagc
     4801 gcatgggggc tcggtatcgg ttcgttccca tgccgcggaa gggactatat ttcgcttcga
     4861 tttaccaacc gtgggggtgg gccgaagggc agaggcgcgg taggatcagt cttccgtgag
     4921 tcgtttgagt tcttctttga tgatggtttc ggcaagtacc ggaacgactt cccagacgac
     4981 gcgttccacc aattcatgag acagggcgag cacaccttcc atctggtctt tggtgaggcc
     5041 gagggcttgt agtttggcgg ataaatcccc atcggctttg gaggattggg ccatgacggg
     5101 tttgacaggt tctttggcag gttccgcgga tggtttggca ggccaagccg cttccacggg
     5161 ttttgcctga acaaccgcgg gaggcggcgt tcccatcgtg gtcgtagagg gcggcaaggg
     5221 cgctcgtgcg gagggaccag cggcaggacg caccgcaaaa tcagccgtcg cccgtaaatt
     5281 gaccggctga ttggcggtgg gagccgcaat gggcgctggt cgagtgactt gcggacgcgg
     5341 ggggccttgc cccaacaacg tcgcgccttg aggcaccgag gcacgaggtg cggtggcagc
     5401 cggtttggct gcgctcggtg aggccacggt cttctgtcct gacaacagac tattcacccg
     5461 ctcgatcagc ttctgggagt cgaagggctt atccacattg tcatccgcgt tcgcggcttg
     5521 gcctcgtgct ttgtcgtagg gattgagttt cgacgacatc agcagaacgg gaagttccgg
     5581 ttgagcctgt ttgagggatg cgcaaagctc ataaccgttt ttgtcgggca acgtgatgtc
     5641 cgccaacaca agggatggct tctcgcttct gaacagggcg agcgccgttt cggcactatc
     5701 cgcggtcagt acacgaaaat cctctcctcc gaaggtcatc tcgtagacct tgcgcatggt
     5761 cacgctatca tcgaccgcga gcagtgttac aggcacggga aggcctcctc ataatccacg
     5821 gttctggcac cggaagatcg gagcgacccg ccgacgcaca ttgaatcatc atggttttcc
     5881 acgtgaattg tcaactctat ggcaattttc accgactcga cctcttgcgg atccatgctt
     5941 tgccctggat gctatcgccg gaagacggaa gccagcaacg ggaaagacca actcctacca
     6001 acccgccgca tgccatgagg aacgcaaggt gacctatcac gtcaaaacct gttttcggac
     6061 gattcaggga gaaggagccc aaacaggccg ggtagccgtc ttttgccgat tgtccggctg
     6121 caacctgtgg agtggtcgaa tcgatgaccg agatcgcgcg atatgcaagt tttgcgatac
     6181 cgattttgta ggcaccgacg gcccagcggg gggcagtttc gccacggcta ccgacctggc
     6241 ggagcatatt gcggtgatct ggcagcagca gaccgtccaa gatgaacatc gctatgtcgt
     6301 ttttaccggc ggggaatctc
//
LOCUS       DAOSOR010000332         6290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_12508_length_6290_cov_4.068805, whole genome shotgun sequence.
ACCESSION   DAOSOR010000332 DAOSOR010000000
VERSION     DAOSOR010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12508_length_6290_cov_4.068805"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1511
                     /locus_tag="PK710_16340"
     CDS             <1..1511
                     /locus_tag="PK710_16340"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOT11352.1"
                     /translation="FLCAVLLLGAGGVGASRLGTEFLPELDEGDILVFVEMPSSISLD
                     DGQQLLRDVRRRLLAFPEVLMTTSQQGRPEDGTDNESVNMSETFVRLKPKSQWRKGMT
                     KPRLEESMREALSEIPGVRFNFSQPIRDNVEEAMSGVRGQVVLKIFGTDLAVMRDTLQ
                     RAIEVLQPMAGVVDLSLYRDREVPQLEIRTDRAAIARAGISVDVVQDVIETALAGKIV
                     TELWEGERIVPIRVQLAPGDREDVSRIAEIPVSNDRGTVYPLGHLSVIDAGTGRASIN
                     RENNSRSLALKFNVEGRDMGSAVHEAIETVRQKIIVPEGTYLQWGGEFENQQRAMGLL
                     MIIAPLALLVVVSLLCVGLRSFRGGAAVLIVMPVALTGGIFSLLFTGVHASISAAIGF
                     VTLLGQVSLMALLILGAIDEERQRGMSLHEAIRTGSVRRMRAVLLASLLAVLGLMPMA
                     ANSGMGSETQRPFALVIVGGMSTTLLVSLLVLPIIYSLLASPVVSPSKQEES"
     gene            1508..2794
                     /locus_tag="PK710_16345"
     CDS             1508..2794
                     /locus_tag="PK710_16345"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT11353.1"
                     /translation="MIREATAGPLVMMVLALATASPATAESQRNHSENLPSIVTLPQT
                     LRLLRDRSPESKALRARLDVVAAERMEADLYPNPSVSYGGLALAQGAPVAPRWQHQIT
                     VEQPVLVFGQRSAKQAWADQLLHAERAQVEASLAEQALLASQAFYALLAAQQRKELAE
                     WSLRDLENFETIVQRRTEAGDRSLYDLAKIQLETSLQRTLLQTVETERIHAVGTLAAR
                     LGFPDWRPTAQGSLEPLMLPISKEKLREYAIRHHPTIQAARARQASAQSSLALARRER
                     YPTPTVALGTLLTQHENSTSVFLGLSVPVAVFDHGQAAVAKATASIRAEQRQGDAELQ
                     RVFAELDRAYQVYERHRATLASLDRQMIEKIPVLEKMAEDSYRGGGSGIWDWLDTHRS
                     LMGLRLLHLEQREAVKQAEVSLWAAAGMAFSAPASP"
     gene            2964..3758
                     /locus_tag="PK710_16350"
     CDS             2964..3758
                     /locus_tag="PK710_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11354.1"
                     /translation="MASLRRRAWAIAACCLFTPGLAFAIDPFELQVYDANITPKGQFG
                     LELHSNYTFSGRKTPSYSGELVPDGAFRVQFEPTYGAAEWLELGLHAYTWVSSEGNPD
                     FGGVMIRGLFVVPPESTAPFFFGIKAELGWVTKAISEEAWDQEFRFVLGYSDRVFLAV
                     INPIVGWPLQGSTMFRPEFEPSAKLAIDTQAGFSLGFEHFSSLGAFSDGFDSASEQQH
                     LFFSVLDLTDKQGVPPPFALNIGVGRGLTDATHAKWIAKAVVGFNL"
     gene            3894..5126
                     /locus_tag="PK710_16355"
     CDS             3894..5126
                     /locus_tag="PK710_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11355.1"
                     /translation="MSQNEEDKELSDEQSAESEAIVVAKKKKKRADGEGSAPSRDRNK
                     RVREQAAKKMEQAKESAKSRANRIGATGLDAGEMVDDAMARGFAAFTKWIKTNRRTVE
                     FSAIGLILAGAGYAAWDWYSNKSREEASAALMLATANAAGVVEEKKDDKEKPAADSAD
                     NKRVDSRPHFDSYQALQDEALRTYRVAADGNSRSGAGILARLGEAGVLLERHDFDGAI
                     QAVNAVLATDLAKADESVRMAAHERLGMALEGKGDEAGALAAYATLADSNLDIYKNYG
                     LYHQARLSYEKGKKDEAKDKLHKLKERLKGVDNKNPAGNRYLLAQVDALLRSVDPTAE
                     SLTPPAPGGELTPEQLIQLQKELEKMRQNPPDLEENAPTEPSSDTPAASGSAPPPPAA
                     SAPAPAASAPTPASSGGN"
     gene            5129..>6290
                     /locus_tag="PK710_16360"
     CDS             5129..>6290
                     /locus_tag="PK710_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOT11356.1"
                     /translation="MLNPRPILLPFWLAVGLCTSCDAARQGANPEVPLWENRPGMALT
                     LVSQSQLTFEGRNFDEAYEHGRPTLDPSMRRVFVGSSDHGLYALRAHDGTVLWRFETM
                     GAVQCEPLYDPSDDSVYFGSNDGALYKVSAQNGKLQWRFMSASEVQRKPVLHQGKLYF
                     SNANDTLVALDPKTGQMIWNYHRAPVGGMSVAGYAGPAVLDNRVFVAFSDGHVMAFDA
                     NDGSSLWQPVDLAAELEQIQGYQVPKYFDADTTPLTFRISTGPVVAVATYAGGLYVLD
                     AETGAQAWSNDRVVGVTDLMMWTQPAHPPRDGGPTIPARRLLLASTGTSGLWALDPED
                     GQEVWRLALPAGGVSAPASIAGAIMVTTTRYGIFLISPLNGKVIDGIDLGMTF"
BASE COUNT         1302 a         1744 c         1869 g         1375 t
ORIGIN      
        1 ttttcctttg cgccgtcttg ctccttggcg cgggcggggt aggggcttca cgtcttggaa
       61 ccgagtttct accggaactc gatgaaggcg atattctagt tttcgtcgaa atgccatcga
      121 gtatctccct cgatgatggc caacaactct tgcgcgacgt gcgacgccgc ttgttggcgt
      181 ttccagaagt gctcatgacc accagccagc agggcaggcc cgaggatggc acggacaacg
      241 aaagcgtgaa tatgtccgag actttcgtcc gtctcaaacc caagtcccaa tggcgaaaag
      301 ggatgacgaa accacggctc gaggaaagca tgcgcgaagc gctttccgaa atcccagggg
      361 tccgcttcaa cttctcccaa cccatccgcg acaacgtcga agaagccatg agcggtgtcc
      421 gaggacaggt ggttctcaaa atattcggaa ccgacctggc ggtgatgcgc gatacgctac
      481 aacgagccat cgaagtgctc cagcccatgg cgggtgtcgt cgacctttcg ctctaccgcg
      541 accgggaagt gccccaactc gaaatccgta cggaccgagc cgccatcgca agagctggga
      601 tcagtgtgga cgtcgtacaa gacgtcatcg aaaccgcgct ggccggcaaa attgtcaccg
      661 agctttggga aggggaacgg atcgtgccca tccgtgtcca actcgcgccg ggggatcggg
      721 aagacgtctc tcgaatcgct gaaatccctg tttccaacga tcgtgggacc gtgtatccgc
      781 tcgggcacct ttcggtcatc gacgctggaa cggggcgagc atccatcaat cgggaaaaca
      841 atagtcgttc ccttgccttg aagttcaacg tggaggggcg cgacatgggt tccgcggttc
      901 acgaggccat cgaaaccgtt cgtcaaaaaa tcattgttcc tgaaggcact tacctgcaat
      961 ggggagggga gttcgaaaac caacagcgag ccatgggcct gctcatgatc attgcccccc
     1021 ttgccctttt ggtcgtcgta tccttgcttt gcgtgggatt acggtctttc cgaggaggcg
     1081 cggccgtgct catcgtcatg cccgtagccc taaccggtgg catcttttcc ctgttgttta
     1141 ccggtgtcca tgcttcgatc agcgccgcca tcgggtttgt gaccttgctt gggcaagtat
     1201 cgctcatggc tttgctcatc ttgggcgcta tcgatgaaga gcggcaacgt ggtatgtcac
     1261 ttcacgaagc gatccgaacg ggctcggtcc ggcgaatgcg agcggtgttg ctcgcttctt
     1321 tgttggctgt actcggtttg atgcccatgg cggccaattc ggggatgggt agcgaaaccc
     1381 aacggccttt cgccctggtc atagtagggg gcatgtccac gacgctgctg gtttccttgc
     1441 ttgtgctacc cattatctat tcgttgttgg caagccctgt cgtatcgccc tcgaagcagg
     1501 aggaatcatg attcgtgaag cgaccgcagg gccgctggtg atgatggtgc tggcgctggc
     1561 gacagcatct cccgccactg ctgaaagtca gcgaaatcat tcagaaaatc tgccttccat
     1621 cgtgacctta ccgcaaacgt tgcggcttct tcgcgataga agtccggagt ccaaagcgct
     1681 tcgggcccgc ctggatgtcg tcgcggccga gcggatggaa gcggatcttt atcccaatcc
     1741 ttcggtgagt tatggggggt tggctcttgc gcagggggca ccggtggctc cccgctggca
     1801 gcatcagatc acggtagagc aaccggtgtt ggtattcgga cagcgaagtg ccaaacaagc
     1861 ctgggccgac caacttttgc atgccgagcg agcccaggtc gaagcgagcc tcgcggagca
     1921 agcgttgctg gcgagccagg cgttttatgc gctgctggcc gcgcaacaac gaaaagaact
     1981 tgcggaatgg agccttcggg acctggagaa ttttgaaacc atcgtgcaac ggcgaacgga
     2041 agcgggagac aggagccttt acgatcttgc caaaatccag ttggaaacga gtctccagcg
     2101 cacactgctg cagacggtag agaccgaacg aattcatgcg gtgggaaccc ttgccgcaag
     2161 gctgggtttt ccggattggc gtcccaccgc ccaggggagt ctcgagccct tgatgctccc
     2221 tatcagcaag gagaagctta gggaatatgc catacgacat caccccacca tccaggcagc
     2281 gcgcgctcga caagcctccg cccagagcag cctcgcgttg gcacgtcgcg aacgttatcc
     2341 cacgcccacg gtagccctgg gaaccctgct tacccaacac gaaaacagca cctctgtttt
     2401 tttgggccta tccgttcccg ttgcggtttt tgaccatggt caggccgcag tcgcaaaggc
     2461 aaccgcatcg atacgagccg agcagcggca aggggatgcg gagcttcaac gagtttttgc
     2521 tgaattggat cgcgcttatc aggtgtacga gcggcaccgt gcgactttgg cttcgctgga
     2581 tcgtcaaatg attgagaaaa taccggttct tgaaaaaatg gcggaagatt cttatcgggg
     2641 aggtgggtcc gggatttggg attggctgga cacccaccgt tccctgatgg gattgcgcct
     2701 tttgcatctg gaacagcgcg aagcggtcaa gcaagcggaa gtgagcctat gggcggcggc
     2761 ggggatggcc ttttcggcac cggcttcccc gtaagtgctc gaaaacagcc aatatttacg
     2821 acaatccatg cccagcgtgg aagtgctatt cctccttgac agacgatgct ttcgtatgta
     2881 gtttcgtcta caaacacgat ccgacttctc cctctgacgg ttctggtagg aaagtatcgg
     2941 aattccacga ttggaggtca attttggcca gccttcgccg ccgtgcctgg gcgatagcag
     3001 cgtgttgcct gtttactccc ggtctggcat tcgccatcga ccctttcgag cttcaggtat
     3061 acgatgcgaa catcacgccg aaggggcaat tcggtctcga actccatagc aattacacgt
     3121 tttccggcag gaaaacgcct tcctattcgg gggagctcgt gcccgacggt gcatttcgcg
     3181 tgcaattcga gccaacctac ggcgcggccg aatggctcga actggggttg catgcgtata
     3241 cgtgggtttc ttcggagggc aatccggact tcgggggagt gatgattcgc ggtcttttcg
     3301 tggttccgcc ggagtcgacc gctccctttt ttttcggcat caaagccgag ttgggatggg
     3361 taaccaaggc catcagcgag gaggcctggg atcaggagtt tcgtttcgtc ttggggtatt
     3421 cggaccgggt tttcctcgcg gtcatcaacc caatcgtggg ctggcccctg caaggatcga
     3481 ctatgttcag accggagttc gagccttccg ccaagcttgc catcgatacc caagctggtt
     3541 tttcccttgg ttttgagcat ttctcgagtc ttggggcgtt ttccgacggt ttcgattctg
     3601 cgtcggagca gcagcatttg tttttctccg ttctcgatct caccgacaaa caaggcgtac
     3661 cgcctccttt tgccttgaat atcggagtgg gaaggggctt gaccgacgcc acccatgcga
     3721 agtggatcgc gaaagccgtc gtggggttca atctttgaga ttttctacaa aattcaataa
     3781 aatcagctaa ttgcgcctac caggcacacc ttctctcacg gcggtcgagc attttgactt
     3841 cgcgacgaaa aatcctgttc cattctttgc cttaatgact agagtctcgc gccgtgagcc
     3901 agaacgaaga agacaaagag ttgagcgacg aacaatccgc tgaatcggag gcgatcgtcg
     3961 ttgccaagaa gaagaaaaag cgtgcggacg gggaggggag tgcgccttcc agagaccgca
     4021 acaagcgagt gcgcgagcag gctgcgaaaa aaatggagca ggccaaagag agcgcaaaat
     4081 ctcgtgcgaa tcgaattggc gcgacggggt tggacgccgg cgagatggtc gacgacgcca
     4141 tggcgcgtgg ttttgccgcc ttcaccaagt ggatcaaaac caatcgtcgc acggtggaat
     4201 tcagcgccat cggcttgatc ctcgcgggtg ctggttatgc cgcctgggat tggtattcca
     4261 acaaatcccg tgaagaggca tcggccgcat tgatgctcgc caccgcgaat gccgcgggtg
     4321 tcgttgaaga aaaaaaggat gataaagaga agccggccgc tgattccgcc gacaacaaac
     4381 gggtggattc tcgccctcat ttcgattcgt atcaggcgct tcaggatgaa gccttgcgca
     4441 cgtatcgcgt ggccgcggac ggcaacagcc gttcgggagc tggaatcctt gcgcgtttgg
     4501 gggaagcggg ggtgttgctc gaacgccatg acttcgatgg tgcgattcaa gcggtcaacg
     4561 cggtgctggc caccgatctc gccaaggcgg atgaatccgt tcgcatggcg gcccacgagc
     4621 ggttgggaat ggctctcgaa ggaaaagggg atgaagcggg agcgctcgcg gcgtatgcca
     4681 cgcttgcgga ctcgaacctc gacatctaca aaaactacgg cttgtaccac caagctcgtc
     4741 tttcttacga aaaaggcaaa aaggacgaag ccaaagacaa gctgcacaag ctcaaggaac
     4801 gtctcaaggg cgtggacaac aaaaatccag cgggaaatcg ctatcttctc gcgcaggtgg
     4861 acgccttatt gcggtccgtc gatcctactg cggagtcgtt gacacctcct gctcctggtg
     4921 gcgaactcac gcccgagcaa ttgatacaac tgcagaaaga actcgagaaa atgcgtcaaa
     4981 acccgcccga cctcgaggaa aatgcgccta ccgagccgtc ttcggatact cccgcggctt
     5041 cggggtccgc accgccgccc cccgcagcat cggcacccgc tccagcggcc tcggcaccca
     5101 cgcccgcatc atccggaggc aattgagcgt gctcaaccca cgccccattc tacttccgtt
     5161 ttggctcgct gtcggcctct gtacttcttg tgatgccgcc aggcaaggcg cgaacccaga
     5221 agttcccctg tgggaaaacc gtccgggcat ggcgctgacg cttgtttctc aatcccagct
     5281 tacgtttgaa ggccgcaact tcgacgaagc ctacgagcat gggcgtccca ccctggatcc
     5341 atcgatgcgc agggtatttg taggctcttc ggatcatggg ctgtatgccc tccgagcgca
     5401 cgatggcacg gtactttggc gattcgaaac catgggtgcc gtgcaatgcg agccgctcta
     5461 tgacccttcg gatgattccg tctatttcgg ttcgaacgat ggggcgttgt acaaggtatc
     5521 tgcccagaac ggcaagctgc agtggcgatt catgtccgct tcggaggtgc aaagaaaacc
     5581 tgttttacat caaggaaagc tctatttttc caatgcaaac gatacgctcg tagccttgga
     5641 ccctaaaacg gggcagatga tctggaacta tcatcgtgct cccgtgggtg gaatgtcggt
     5701 ggccggctac gcgggtccgg cggtgctgga caatcgggtg ttcgtagctt tttcggacgg
     5761 tcacgtgatg gctttcgatg ccaacgatgg ttcttcgctt tggcaacccg tggatttggc
     5821 tgccgaattg gagcaaatcc agggctatca ggtgccgaaa tactttgatg cggataccac
     5881 accgctgacg tttcggattt ccaccggtcc ggtggtagcc gtggccacgt acgcgggtgg
     5941 attgtatgtt ctcgatgcgg aaacgggcgc ccaggcctgg tccaatgaca gggtggtggg
     6001 cgtgacggat ttgatgatgt ggacgcagcc tgcgcatccg ccgcgcgacg gtgggccgac
     6061 catcccggca cggcgattgt tgttggcttc gacgggcacc tcgggacttt gggctttgga
     6121 tccggaagat gggcaggagg tttggcgatt ggcgcttccc gcggggggag tctcggcgcc
     6181 ggcctcgatc gcgggggcca tcatggtcac cacgacgcgt tatggcattt tcctgatttc
     6241 accgctgaac ggcaaggtga tcgacggcat tgatttgggg atgaccttct
//
LOCUS       DAOSOR010000333         6261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_12584_length_6261_cov_4.636642, whole genome shotgun sequence.
ACCESSION   DAOSOR010000333 DAOSOR010000000
VERSION     DAOSOR010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12584_length_6261_cov_4.636642"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1674)
                     /locus_tag="PK710_16365"
     CDS             complement(<1..1674)
                     /locus_tag="PK710_16365"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOT11357.1"
                     /translation="MNRPRELTFEFLCDILEHDKLITFEQRRDAQLREDRERVRIMRS
                     QQSRSRDVTAVHPTTVLASLGLFVGGDSRYPLTEQAITQALARHIGVPYVHLNPLDID
                     AKLAVQTLSRPFARRHNALVIGGDDSHVTVAVANPLDVELIDNIKQFVRRHVDFVLAP
                     PGDIQRIITDIYGFRTSVKAAEREFTQGVDIGNLERYIKLKRVDEIEATDSHIINAVE
                     YLFHYAFDQRASDIHIEPHRDQSVVRMRIDGVLHTVNVLPKVVHPAIVSRLKTLSRLD
                     IAEKRRPQDGRIKTARGQREVEIRVSTMSVAFGEKVVLRIFDPEIVLRDLSDLGLFDK
                     QLATFEQFIQRPHGLVLVTGPTGSGKTTTLYSALRAVSSPQVNVNTIEDPIEIVVDQF
                     NQVEVQHKIGITFAESLRHILRQDPDVIMVGEVRDVDTANIAIQASLTGHLVLATLHT
                     NDSASAITRLLELGVDPFLLSSTLVGVLAQRLVRMICPECRVETFLTPDQMSLLGLDV
                     DQLTEQGESPQLMVAFGEGCVKCRNTGLYGRSGVFEVLPVDDKIRRMIAT"
     gene            complement(1754..2407)
                     /locus_tag="PK710_16370"
     CDS             complement(1754..2407)
                     /locus_tag="PK710_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11358.1"
                     /translation="MRLHQVLDLIGELPTSQMQEVWDAAAPMAGTDLRDGVWLGRYGG
                     LAWPIKRIVRHVVRRDYFARLVFPGWGVNVRVKQDGTYAMIPWSAVPVGVRVHLPFRR
                     TNQGLDYGYHVTGIDVGPSSWLPAREVLRKIDFSTLSEVVSLGHLRRVGVWRGKAEHP
                     GQLVVGYLAPVGVEAFKHSPFAMVWRREMSTAEVRSAEHHIHRMRLWDTSAGSIDMS"
     gene            complement(2480..2983)
                     /locus_tag="PK710_16375"
     CDS             complement(2480..2983)
                     /locus_tag="PK710_16375"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOT11359.1"
                     /translation="MLSNEMIEKLNEQITHEFYSSYLYRQMAAWFAEQNLTVLQEYFT
                     THAAEEEMHATKLIDYVLKAGGHVKLGAIAAPSDTFSSPLDVMTKTLEHERGVTRKIH
                     ELMAQAEKDRDYSTRSYLQWYIDEQVEEEATFGELLALTRMAGDHLLLLEERIEKRAA
                     KRGVATE"
     gene            complement(3155..5758)
                     /locus_tag="PK710_16380"
     CDS             complement(3155..5758)
                     /locus_tag="PK710_16380"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT11360.1"
                     /translation="MRAAFLIAPTYEACDEFRPHLHAAYDARAVGERLLEPGYDFEVF
                     EAPKGVEPEVWLTEILKDKGQGETDALLVYVSAVSHLDEAGELSIEVMTGRATSRLPF
                     TKLRTAISSRGSRSVVVVLDLLMDGPMDAVVATEYTAAARRVWAPELSGYSMLCAVRG
                     RQPGTTPSVSPFTSLWLSAMADPQSRNKIGVAFISRVMDVMRENENLYTDVPCFSLVP
                     GRRDLKVYSTTSPSLTRPSSSSVDRVSSPPSVRQPFVRVDDVFSEGDALLEAGDWEGA
                     SKVFKKILLLLEEKSVERAHAYVRLGAIKRLQNRPREAAIHYRKALGVDGRHPEALER
                     LADVLRSEGEFAEAATYRRALLGVVQDDHRRFEVLLCLAEDFEKARDLARCIEALEDA
                     RSIQPEETAILARLAQVYDVSHQPDKVVDIKVAIAQLKMQPEEVARSLVLAADYAVER
                     AQDVERALLIYEDALDRDPLTPRAFDAIAHIWIAREDLRAYEKALLAQTVRLDKAFAH
                     AAEAEVWREIALLRHDKLDDLRGAIEALDRCVERVPGDVEARVALSELLVKNGEMEAA
                     IMCLEIAAWHAPGRASTYRMMHELMLKVGWIDRAYCCAAVLVLLEEADLDEQLFYEQY
                     HPEVRVNPKHSLPAKGWAKLYPAGSESNLGPILELVASHAIEYKLSWLKKNGMLPDLD
                     PSMRQDPETSTVSLTRTFVWASTILDVPLPEIYVGDDVPGGIAAVPEQLPTAIVGRSV
                     LSGRSLKELGFLVGRDIAYFRPLHYLLVLYSSLKDLTALFLASICVVRPDYAVPDHVK
                     REIKELTRFLRDHLSEKESAALNHAVERFEQDGVRADLVGWARSRDGVDASGATSLRR
                     HCRGQSSVGDR"
     gene            6004..>6261
                     /locus_tag="PK710_16385"
     CDS             6004..>6261
                     /locus_tag="PK710_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11361.1"
                     /translation="MRTQLLPIPACAALAVCVFTYHAMGQQPSDSDSAPGGETGTSAE
                     PSPAPEASPDTMQPAEAPPEPAGPPTPTTETQCSDGIDNDGD"
BASE COUNT         1480 a         1990 c         1531 g         1260 t
ORIGIN      
        1 cgtggcaatc atacgccgaa ttttgtcatc gaccggcaat acttcgaata ctcccgaacg
       61 accatacaac ccggtgtttc gacacttcac acacccttcg ccgaatgcca ccatcagttg
      121 aggcgactcg ccctgctccg tgagctgatc cacatccaat ccaagcagag acatctgatc
      181 gggcgttaga aacgtttcca cacgacattc cggacaaatc atgcgcacca accgctgcgc
      241 aagcactccg accaacgtcg acgagagcaa gaatgggtcc acgcccaact ccaatagcct
      301 ggtaatcgcc gacgctgaat cgttggtgtg caacgtggca agcaccagat ggccggtgag
      361 cgacgcttgg atcgcaatat tcgcggtgtc cacatcccgc acctcaccga ccatgatgac
      421 atccggatct tggcgcaaaa tatgacgcaa cgattcggca aacgtaatgc caattttgtg
      481 ctgcacttcc acctgattga actgatccac cacgatctcg atcggatcct caatcgtgtt
      541 gacattgact tgcggagacg acacggcacg aagcgccgaa tacagcgtgg tggtcttgcc
      601 gcttcccgtc ggtcccgtca ccagaaccag cccgtggggg cgttgaatga actgctcgaa
      661 cgtcgccaat tgcttgtcga agagtccaag atccgaaagg tcccgcagca cgatttcggg
      721 atcgaaaatc cgcaagacaa ccttttcgcc aaacgcaaca ctcattgtcg acacacggat
      781 ttccacctcc cgttgcccac gcgcggtttt gatccgacca tcctgcggcc gtcttttctc
      841 cgcgatatcc aagcgagaca gcgtcttcaa acgagacaca atcgccggat gcacgacctt
      901 cggaagcacg ttgaccgtat gaagcacgcc atcgatgcgc attcgaacca cggattgatc
      961 acgatgcggc tcgatatgaa tgtccgaagc cctctgatcg aaagcgtaat gaaaaagata
     1021 ctccaccgcg ttgatgatat ggctatcggt agcctcgatc tcatcgaccc ttttcagctt
     1081 gatgtaccgc tcgaggtttc cgatatccac cccttgggta aactcccgct ccgcggcctt
     1141 gaccgaggtg cggaacccgt aaatatccgt aatgattcgt tgaatatccc cagggggcgc
     1201 caacacgaag tccacgtgcc ttcggacgaa ttgtttgatg ttgtcaatca actccacatc
     1261 gagaggattg gccacggcca cggtgacatg cgaatcatcg cccccaatga ccagcgcgtt
     1321 gtgccggcgc gcgaaagggc gtgacagggt ttgcaccgct aacttggcat cgatatcgag
     1381 aggattgaga tggacgtagg gaacgccgat atggcgcgcc aacgcctggg tgatggcttg
     1441 ttccgtgagc ggatatcggc tgtccccccc tacaaataat ccaagggaag ccaacacggt
     1501 cgtgggatgc actgccgtca cgtcccgact acgagactgc tggctgcgca tgattcgtac
     1561 tcgttcccgg tcctctcgca actgcgcgtc acgtcgctgc tcgaacgtga tgagcttatc
     1621 atgctcgagg atgtcgcaaa gaaactcgaa agtcagctca cgaggtcgat tcacagcatc
     1681 agcgtgcgca aggacaggtc acgccgtcaa gctttgtcca ttcgccgtgc ccatccacgg
     1741 agcgaatgaa tcatcacgac atgtcgatcg acccggctga tgtatcccac aggcgcatac
     1801 gatggatatg atgctccgcc gatcgaacct ccgcagtgga catttcacgc ctccacacca
     1861 tcgcgaaggg agagtgcttg aatgcctcca cgccgaccgg tgccaggtaa ccgacgacga
     1921 gttgcccggg gtgttctgcc tttcctctcc acacgccgac gcgacgcaaa tgccccaggc
     1981 tcaccacctc cgatagcgta gaaaaatcaa tttttctaag cacttcgcgt gcaggcaacc
     2041 acgaggaggg cccgacgtcg atccccgtga cgtggtatcc gtaatcgagt ccttgattcg
     2101 ttcgacggaa aggaagatgc acgcggacgc ctaccggcac ggccgaccag gggatcatgg
     2161 cgtacgttcc atcctgcttc acacgaacgt taacgcccca ccctggaaag acgagccttg
     2221 cgaagtaatc ccgtcggacg acatgccgaa cgattcgctt gattggccaa gccaatcctc
     2281 cataccgtcc aagccagacc ccatcacgaa gatccgttcc ggccatgggt gcggcagcgt
     2341 cccaaacctc ttgcatttgc gaggtaggaa gctcaccaat caggtccaac acctgatgta
     2401 gtctcatggc ggcattttgt gctggggttt ccgtttcatg caatgaaaat gggtgcgacg
     2461 cgcgcgactc gtggcgctct tactcggtcg ccacccctcg tttcgcagct cgcttctcga
     2521 tgcgttcttc gagcaacaaa aggtgatccc cagccatacg cgtcaacgct agaagctcgc
     2581 caaaggtggc ttcctcctcc acctgctcat cgatgtacca ctgaaggtag cttcgcgtgg
     2641 agtaatcccg atccttttcc gcctgagcca tcaattcatg gatttttcgc gtcacacctc
     2701 gctcatgctc gagggtcttg gtcatcacgt ccaaaggaga actgaatgtg tcagaaggtg
     2761 ccgcaatggc gccaagcttc acgtgtcctc ccgccttgag cacgtagtcg atcagcttgg
     2821 tggcgtgcat ttcctcttcc gcggcgtggg ttgtgaaata ctcctgcagt acggtcaagt
     2881 tctgctccgc aaaccaagcc gccatctgcc ggtacagata ggaagagtaa aactcgtgcg
     2941 taatctgttc gttcaacttc tcaatcattt cattggacaa catggcatgt ctcccatccg
     3001 tcaaggtcat tgcgttgcat ccaccacaac gcaaccccag cttcatccca ccgcaatccc
     3061 aagcattctc cgtaattccg cgtaggattc agaaaccgaa aacggaatca gatcatgaat
     3121 gcgatcgcga gaagtcaagt cacatacact ccgatcatcg gtctccaaca cttgattgac
     3181 cacggcaatg tcgccgcaaa gaagtagccc cgctcgcgtc gacgccatct cgggaacgcg
     3241 cccatcccac gagatccgct cgcacaccat cctgctcgaa tcgctcgacg gcatggttca
     3301 acgccgcaga ctctttttcg ctcagatgat cgcgcaagaa acgagtcagc tccttgatct
     3361 ctcgcttcac atgatccgga accgcataat cggggcgtac gacacaaatc gacgcaagaa
     3421 atagtgcggt caagtccttg agactggagt aaagcacgag caaataatgc aaggggcgga
     3481 agtaggcgat gtcacgaccg accaaaaacc ccagttcttt caggcttcgt ccactcagca
     3541 ctgaacgtcc gacgatcgcg gtgggcaatt gctccggcac ggccgcaatc cctccgggaa
     3601 catcgtcacc cacgtaaatc tcgggcagtg gcacatccaa aatggtcgac gcccacacga
     3661 aggtgcgcgt caaactcacg gtactcgtct ccggatcctg acgcatgctt gggtcgaggt
     3721 cgggtagcat cccgtttttc ttgagccacg atagcttata ttcgatggca tgagaggcca
     3781 cgagttccaa tatgggccca aggttggact cggagcccgc gggataaagc ttcgcccagc
     3841 ctttcgcagg caacgaatgc ttcggattga ctcgcacctc agggtgatac tgctcataga
     3901 ataattgttc gtcgaggtcg gcttcttcga gaagaacgag caccgccgca caacaatacg
     3961 cccgatcgat ccagcccacc ttcaacatca gctcatgcat cattcgatag gtcgaagcgc
     4021 gaccaggcgc atgccatgcg gcgatttcca aacacatgat tgccgcttcc atctcaccgt
     4081 ttttcacaag caactccgac aaagccacac gcgcttccac atcgccagga acccgctcca
     4141 cacatcgatc caatgcctcg atggcaccgc gcaaatcatc cagcttatca tggcgaagca
     4201 aagcaatttc acgccacact tctgcctccg ccgcatgcgc aaatgccttg tccaatcgga
     4261 ccgtctgcgc caataacgcc ttttcatagg cacgaaggtc ttcacgcgcg atccagatat
     4321 gagcaatcgc atcgaaagct cgtggggtaa gcggatcccg atcgagcgcg tcttcgtaaa
     4381 tcaaaagcgc gcgttctaca tcctgcgccc gctcgacggc ataatccgcc gcaagcacca
     4441 gggaacgcgc cacttcctca ggttgcattt tgagttgcgc gatcgccact ttgatgtcca
     4501 ccactttgtc cggctgatgc gaaacgtcat aaacctgcgc taaacgcgcc aaaattgccg
     4561 tctcttcagg ttgaatcgaa cgcgcatcct ccaacgcctc gatgcaacga gccaagtccc
     4621 gcgctttttc aaagtcctcc gcaagacaca gcaatacctc gaatcgtcgg tgatcgtctt
     4681 gcaccacacc gagtagcgcc cgacgatacg tggctgcctc ggcaaattct ccttcgcttc
     4741 gcaacacatc ggccaatcgt tccaacgctt ccggatgccg cccatcgacc cctaacgcct
     4801 tacgataatg gattgccgct tcccgcggcc gattctgcaa tcgtttgatc gcgccaagcc
     4861 tcacgtaggc gtgcgctcgc tccacactct tttcttcgag aagcagcaat atttttttga
     4921 ataccttcga cgctccttcc caatcccccg cctccaacaa ggcatcccct tcggaaaaca
     4981 catcgtcgac ccgaacgaaa ggctgcctca cactcggagg cgatgacacc ctgtccaccg
     5041 atgacgacga gggcctcgtc aatgaaggcg aagtcgtcga atatactttc aagtcccgtc
     5101 gccccggcac caacgaaaag cacggcacgt ccgtatacag gttttcgttt tcccgcatca
     5161 cgtccatcac gcgagagatg aaggcaaccc ctatcttgtt tcgcgattga ggatcggcca
     5221 tggcagaaag ccataacgac gtaaacggcg acaccgaagg tgtcgtcccg ggttgtcgac
     5281 cacggaccgc acaaagcatc gaataccccg ataattcagg ggcccaaacg cgacgagcgg
     5341 ctgcggtgta ctccgtggct acgacggcgt ccatcggtcc gtccatgagc aagtcgagca
     5401 caaccacgac actgcgacta cctcgggaag agatagccgt ccgcagcttg gtgaacggaa
     5461 gcctcgacgt ggcccgacca gtcatgacct cgatcgataa ttcacccgct tcgtctaagt
     5521 gactcacggc ggagacatag accagcaagg catcagtctc cccttgcccc ttgtctttca
     5581 agatttccgt aagccaaacc tctggctcga cccctttggg cgcctcaaat acctcaaagt
     5641 cgtagccagg ttcgaggaga cgttcaccca cggctcgagc gtcgtaggca gcatgaagat
     5701 gggggcggaa ttcgtcacac gcctcgtaag taggcgcaat caggaacgca gctcgcatgg
     5761 ttttcccatt gtcgaacgaa aacaccgggg ttcgcgcgga ccaaactccg caagaaaaca
     5821 atactctggt gatcaaccat cgtaagtcaa cgggttcgag gattcgaaaa cagattgcac
     5881 cacgagccaa tcaatcgcgc ggcctcgaaa ccaatcccca ttcccacccg tgtagggaaa
     5941 agaattgctc tttaaccgca ctccaccatt gccaactaga gccgttgcga cgatagtggg
     6001 cccatgcgta cgcaacttct gcccatacct gcctgcgcgg cgctcgccgt gtgcgtattc
     6061 acctaccatg ccatgggtca acaaccatcg gacagtgata gcgctcccgg aggagaaacc
     6121 ggcacttccg ccgaacccag cccggcaccc gaggcttccc ctgacacgat gcaacccgcg
     6181 gaagcccccc cagaacccgc cggccccccc acacccacga cggaaacaca atgctccgac
     6241 ggcatcgata acgatggcga t
//
LOCUS       DAOSOR010000334         6244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_12632_length_6244_cov_4.137502, whole genome shotgun sequence.
ACCESSION   DAOSOR010000334 DAOSOR010000000
VERSION     DAOSOR010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12632_length_6244_cov_4.137502"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2671)
                     /locus_tag="PK710_16390"
     CDS             complement(<1..2671)
                     /locus_tag="PK710_16390"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT11362.1"
                     /translation="MNETYGRYRLIERLGKGGMAEVFKAKSFGVEGFEKVLVVKRILP
                     ELSRNARFVELFIHEAKLSVHLSHANIVQAFDLGMVGSNEDAVLPSYFIAMEYVAGLD
                     LAALLSRMRRRSVPLPIGLCVFVASEVCKGLEHAHRRCDERREPLGIVHRDVSPQNVL
                     LSWEGDVKLTDFGIAKARDWQRGQPEGSLAGKAAYMSPEQARGESVDARSDIFSLGTV
                     LYEMVTGTNPFRAASTGETLARVQTDSPTPVHALRQGVDPSLSAILGRAMALSARERY
                     SSAGVFHDELLGFMYATGTRFSSNDLIEYLAPLRASAGEELDVEGLLVDKTQPDGDDI
                     TPVEVPDARASVPPVRVTTTLPTYSYASTVGERREVTVLVIRTGVALSVAQSQRVKDT
                     LARYGARLTDETRDGLVALLGLGEADGRETETAVRCALVVLRQLSTGRWGASAGVHTG
                     RVQLHDSGEPARDDTYASIIEVASKLAMAQSGRCVVSKLAARQVRSLFELEPWDTDDG
                     VMGSLVGEPRLSDDALRRFVGREDEYRQMGKVLAAAARRRLKIVTIRGDQGIGKTRFV
                     DALERRLKRSAYHIGMYVACCPPLGKQMPLSGLSSMLRVLCGVKEGDGPERIATIEPR
                     LRALGLTDEERGAVLGQLGALATDMVGPSVPPLRSAFSRMLQRLSEDRVHAFVWDDAQ
                     ALDDDTFDVLMSAAKRLAGARAVLMFCTRSYAPHPLADLDEHSYVDLVELNDKEVSDL
                     IAARAGLAHAPEELVAFCQQRAGGHPLFIEELIKELMETEALVVQQGQVQRLRLTGDI
                     AAPRPLKSLLAGRLSRLEDSCKMVLHAMAVLASPAHVEVLSMMLGMGLMPVETAIATL
                     EERGLVRRVGPSTSSLGSPLLREVVVDSIAPEGR"
     gene            complement(2732..3568)
                     /locus_tag="PK710_16395"
     CDS             complement(2732..3568)
                     /locus_tag="PK710_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11363.1"
                     /translation="MNAQGMNAIGISSTSAASASNSSATSALQAVADQLRSQIAKFER
                     QGGSQRQLAEAVVALMDEGLSANPPVLALASRMAWLIAVYAPCYDPVLVTTLPFCLES
                     ILDGYASFDDLIDHVLASQPDASVAPLPDDFLIRPARDLAEQAHRGALTLRDDPAVYE
                     RIIDRLMVGDVGEIVSNRKRPAGEKYMNSASNDAFHGGERTVLTRWPTALREAAVSVL
                     QVEPSPRAVPLMCQITERLDARSVRRLAVELVHISADGDNLRIRGRAAASLLAMSKHH
                     RF"
     gene            complement(3909..>6244)
                     /locus_tag="PK710_16400"
     CDS             complement(3909..>6244)
                     /locus_tag="PK710_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOT11364.1"
                     /translation="GTCSVTAGSGSTVYRGIVLAPDDVYEGGEVLVDSKGMIQCVGCD
                     CSGDPSYAAATKVVCPKGVISPGLINAHDHVTFGNNRPYGTGDYNALKDVRYQHRHEW
                     RKGKNGLPKIPVSGSASQAVQTGVELRFMMSGATSINGSNGAKGILRNLDRDDKNDGL
                     GLGALEYQTFPLGDNDGQMIANGCNYPKMDTSSWAQGHPFYVPHVAEGINKEARNEFV
                     CLSQGNNNLLFNKAAWIHGVGLVADDVKYFSRTGTKLIWSPRTNVSLYGDTNRLTLFH
                     KAGAIIALGTDWVASGSMNLSRMFDCIRGLNENNFGGYFTDEDLWRMATTNGAFATGT
                     DSVLGMLKKTYVADIAIFNGAKHDKNPHAAVTNAGLTDTVLVLRGGRPMYGDAALLTS
                     LGKGGCEDMPPAADAASNSAWQTGVCGVPKKACVADDVGGGVTLAGVVGALTPIYPLY
                     YCGVPEYEPSCVPMRTNPVSYGGIPTADDQDGDGIPDSADNCPTVFNPIRSLDGNKQG
                     DFDGDGIGDECDVCPFDANNSACEYNWAANDVDCDGIKNGSDNCPYVANAGQEDQDGD
                     GKGDACDKCPTVPNPGAQKCPAGTTSIEQARKTFSTGDDVMFQGVVVTGVRKPVGSSN
                     GFYLSDPNPVPWGCIFVHTGKDTPTVSVGDRIDIKGVLDEYNGLNQLKIGFQITNSTP
                     GAPLAPMVVTVAELVGANAESYESCRVEIQTVSTVTMGSGIAFAQGSSQIRASNFIWA
                     GGAFPTAGKSYASVRGFSSVFGGDREYLPQTDADLTP"
BASE COUNT         1386 a         2058 c         1584 g         1216 t
ORIGIN      
        1 cccgcccctc cggcgcaatg gaatccacaa cgacctctcg caacaagggt gaaccaagcg
       61 acgatgtcga gggccctacc cttcgcacca accccctctc ctcgagtgtg gcaatcgccg
      121 tttctaccgg catcaacccc atgcccaaca tcatcgaaag cacttccaca tgggcaggcg
      181 acgccaacac ggccatggcg tgaagcacca tcttacagga gtcttccaaa cggctcagac
      241 gtccggccaa gagcgacttg agcggacgcg gcgcggcaat gtcgcccgta agacgaagac
      301 gctgcacctg gccctgttgc accaccaacg cctccgtttc catcaactct ttgatcaatt
      361 cctcgatgaa tagcggatgc ccccccgcgc gttgctgaca aaacgcaacc aactcctcag
      421 gcgcgtgagc aagccccgca cgcgcggcaa tcaaatccga aacctcctta tcgttcaact
      481 cgacaaggtc cacgtagcta tgctcatcca aatccgccaa gggatgcgga gcataagaac
      541 gcgtacagaa catcaatacc gctcgcgccc cggctagccg cttggccgcc gacatcaaca
      601 catcgaaggt atcgtcgtcc aacgcttgag catcatccca tacgaacgca tgcaccctgt
      661 cttcactcaa tcgttgcagc attcgtgaaa acgcggatcg caaaggagga acactgggcc
      721 ccaccatgtc ggtcgccaac gcgccaagct gccccaatac ggcaccccgt tcctcatccg
      781 tcaaccccaa cgcgcgcaaa cgcggctcaa tcgtggctat ccgctccgga ccatcccctt
      841 ctttcacacc acaaagcacc cgcaacatcg acgatagccc cgaaagcggc atttgctttc
      901 caaggggcgg acagcaagcc acgtacatgc cgatgtggta cgcactgcgc ttcaatcgcc
      961 gctcgagagc atccacgaaa cgcgtcttgc caatgccttg atccccacga atcgtcacga
     1021 tcttgagcct tcgtcgagca gccgccgcaa gcaccttgcc catctgacga tactcgtctt
     1081 cccggcccac gaaccgtcgc aacgcatcat ccgacaaccg tggttcgccg accaaagacc
     1141 ccatgacccc gtcgtccgtg tcccatggct cgagttcaaa tagacttcgt acctgacggg
     1201 ctgccaattt cgacacgaca catcgccccg attgcgccat cgcaagcttc gacgccacct
     1261 cgatgatcga ggcataggta tcgtcgcgag caggctcccc cgaatcatga agctgaaccc
     1321 gacccgtatg cacgcccgca ctcgcccccc atcgtcccgt cgaaagctga cgcaacacta
     1381 ccaatgcaca gcgaacggcc gtctccgttt cccggccatc cgcttcgccc aagcccaaga
     1441 gcgccaccag cccatccctg gtctcatccg taagacgagc cccatatcgc gccagcgtat
     1501 ctttcacacg ctggctttgg gccaccgaaa gagcaacacc ggtccgtatc accaacacgg
     1561 tcacttcacg acgctcgccc accgtcgagg cgtagctgta cgtgggcaac gttgtcgtaa
     1621 cgcggacagg aggtacgctt gcccgcgcat ccggaacctc caccggtgtg atatcgtccc
     1681 catcgggctg cgttttgtcg acgagaagac cctccacgtc cagctcttca ccagcgctgg
     1741 ctcgcaaagg agccaggtac tcaatcaggt cattcgaaga gaaacgtgtt ccggtggcat
     1801 acatgaaccc aagcaattcg tcgtgaaaga cgccggcaga cgagtatctt tcgcgagccg
     1861 agagcgccat ggctcgcccc aaaatggccg acagcgaagg atcgactccc tgtcgaagcg
     1921 catgaacggg agtcggagaa tcggtctgca cacgcgccaa cgtctcaccc gtagaagccg
     1981 ctcgaaatgg attcgttccc gtcaccatct cgtaaaggac ggtgccaagg gagaaaatat
     2041 cgctgcgtgc atcgacgctt tccccgcgcg cttgctccgg actcatgtac gcggcctttc
     2101 ccgcaagact tccctcaggt tgtcctcgct gccaatccct cgctttcgca ataccaaagt
     2161 ccgtaagctt gacatcccct tcccaggata acagcacgtt ttgaggggat acatcgcgat
     2221 gcacgatgcc taacggctcc cgccgttcgt cgcatcgccg atgggcatgc tccaatcctt
     2281 tgcacacttc gctggcgaca aacacacaca gtccgatggg caatgggacg gatcgtcgac
     2341 gcatgcgaga cagcagcgcg gccaggtcga gccccgccac gtattccatc gcaatgaagt
     2401 acgaggggag gacagcatcc tcgttcgagc caaccattcc caagtcgaac gcctgaacga
     2461 tgttggcatg agacaagtga accgaaagct tggcttcgtg aatgaacaat tcgacgaatc
     2521 gggcgttgcg cgacaactcc ggcaggatgc gcttgaccac cagaactttt tcgaatccct
     2581 ccacgccaaa gctcttggct ttgaacacct cggccatccc gccttttccg agacgttcga
     2641 tcaggcgata tcgtccgtac gtttcattca tgccgcgttc ccaagacagg acgaggagcc
     2701 tagcactggc acgaaggatc acgaacgacg attagaatcg gtggtgtttg ctcatggcga
     2761 gcagcgacgc cgcggcacgc ccgcggatac gaaggttgtc accatcggcg gaaatatgaa
     2821 cgagttcgac ggcaagcctt cgaacgcttc gcgcatccaa tcgctcggta atttgacaca
     2881 tcaacgggac agcccgcggc gatggctcca cttgcaatac cgataccgct gcctcccgaa
     2941 gcgccgtcgg ccaacgggtc agtaccgtcc tttctccgcc atgaaaggcg tcgttcgaag
     3001 cggaattcat gtatttttct cctgcgggtc ttttccgatt cgaaacgatt tccccgacgt
     3061 cacccaccat cagccggtcg ataatccgtt cgtacactgc gggatcatcg cgtaatgtca
     3121 gcgctccgcg atgcgcttgt tccgccagat ctcgagccgg tcgaatcaga aaatcatcgg
     3181 gtagcggcgc aacgctagcg tcaggctgcg aggccaaaac atgatcgatg agatcatcga
     3241 aacttgcgta gccgtcgagg atgctttcca aacaaaacgg cagcgtcgtg acgagaacgg
     3301 gatcgtagca gggggcatag acggcgatga gccaagccat gcgtgaagcc aaagctaata
     3361 ccggcgggtt ggccgatagc ccttcgtcca tcaaggccac cacagcctcg gcgagctggc
     3421 gttgtgatcc accttgccgc tcgaattttg cgatttgact ccgcaattgg tccgcgacgg
     3481 cttgaagcgc ggatgtagcc gaagaattgc ttgccgaagc agcgctcgtg ctggaaatcc
     3541 ctatcgcatt catgccttgt gcgttcatga ccagccgttg accacggccc acggggaggg
     3601 cgtcgccaca ggtattgctt cctcgttttg gtacgctgag ggtcgaacaa ccttccccgc
     3661 atcaaacctc ggatccaaca ggaagacttt cgcgagcgca ccggattggg aatgggaaat
     3721 aggtgagtgc tttcgtggac tcgctgaggg tcgttgtgaa aagcacccga aatagcttag
     3781 ggatccaggg ggtaggatag agaaaaagcc gcgggactca tcggcaaagc aaggggatgt
     3841 tggggagaat cagcgggaaa gccgcaacga accatcaggg ttcgaggcct tcccgcggaa
     3901 gcaatcggtc aaggggtcag atccgcatcc gtttgcggca agtactcccg gtctcctcca
     3961 aagacgctcg aaaaaccgcg tacgctggca tagctcttgc cggccgtggg gaacgctcca
     4021 cctgcccaaa tgaagttgct cgcccggatc tgtgaactgc cctgcgcaaa agcaattccc
     4081 gaacccatcg tcacagtcga caccgtttga atctcgactc ggcaggattc ataagactcc
     4141 gcattcgctc ccaccaactc ggccaccgtc accaccatcg gcgccaaagg cgcccctggc
     4201 gtggaattgg tgatctggaa accaatcttg agctggttca aaccgttgta ctcgtccaac
     4261 acacccttga tgtcgatgcg atcgcccacc gataccgtcg gcgtgtcttt tccggtatgc
     4321 acgaagatgc acccccacgg caccggattg gggtccgata gataaaaccc attcgatgaa
     4381 cccacgggct tgcgcacacc cgtcaccacc actccttgga acatcacatc atcgccagta
     4441 ctgaaagtct tgcgcgcctg ttcgatggat gtcgttcccg ccggacactt ctgcgccccc
     4501 ggattgggaa ccgtcgggca cttgtcacac gcatccccct tgccatcgcc gtcctgatcc
     4561 tcctgcccag cattcgccac atacggacaa ttgtccgacc cattcttgat gccgtcgcaa
     4621 tccacatcat tggccgccca attgtactca caggcagagt tgttggcgtc gaacggacaa
     4681 acatcgcatt catccccgat gccgtcgcca tcgaagtccc cctgcttgtt tccatccaag
     4741 gaacgaatgg ggttgaagac ggtcggacag ttgtccgccg aatccgggat gccatcgcca
     4801 tcctgatcgt ctgccgtggg aatcccaccg taggaaaccg ggttggttcg catcggcaca
     4861 caacttggct cgtattcggg aacaccacag taatacagcg ggtagatcgg cgtgagtgcg
     4921 ccaaccactc ccgccagagt gacgccaccg cccacgtcat cggcgacaca tgcttttttc
     4981 gggacaccgc acacgcccgt ttgccacgcc gaattgcttg ccgcatcggc ggcgggtggc
     5041 atgtcctcgc atcccccttt gccaagggaa gtcagcaagg cggcgtcacc gtacatcgga
     5101 cgcccccctc gtaacaccaa caccgtatcg gtcagccccg cgttcgtcac cgccgcatgc
     5161 gggttcttgt catgcttcgc cccgttgaaa atggcgatat ccgccacgta ggttttcttc
     5221 agcatgccca gcaccgaatc ggtgcccgtc gcaaacgcac cgttggtggt agccatccgc
     5281 caaaggtcct catcggtaaa gtaacccccg aagttgttct cgttcaaacc tcggatacag
     5341 tcgaacatcc gcgacaggtt catcgagcca ctcgcgaccc aatcggtacc caacgcgatg
     5401 atcgcgccgg ccttgtggaa caaggtcaac cgattagtgt caccgtatag ggatacattc
     5461 gttcgcggcg accagatcag tttggtgccc gttcgcgaaa aatacttcac gtcgtcggcc
     5521 acgagcccca cgccatggat ccatgctgcc ttgttgaaca gcaagttatt gttaccctgg
     5581 ctcaaacata cgaactcgtt gcgcgcttcc ttgttgatgc cctcagccac gtgaggcacg
     5641 taaaaaggat gtccctgggc ccaactcgac gtatccattt tgggatagtt acagccattg
     5701 gcaatcatct ggccgtcgtt gtcccccaac gggaacgtct gatactcgag cgctccgagc
     5761 ccaagcccgt cgtttttgtc gtcacgatcc aggtttctca ggattccctt ggcaccgttc
     5821 gaaccgttga tcgacgtggc accgctcatc atgaaccgaa gctcgacccc cgtctgcacc
     5881 gcctgactcg cactgccgct gaccggaatc ttcggtaacc cattcttgcc tttgcgccat
     5941 tcatgacggt gctgataccg aacgtctttc agagcgttat agtcccccgt gccataaggc
     6001 cggttgttgc caaaggttac gtgatcatgc gcgttgatga gccccggact gatcacgcct
     6061 ttcggacaga caactttggt cgctgcagcg tagctcggat cgccggaaca atcacagccg
     6121 acacattgga tcattccttt cgaatccacc aaaacttccc cgccctcgta cacatcgtcg
     6181 ggagcgagaa cgatgccgcg atacaccgta cttcccgaac ccgccgtgac cgaacacgtg
     6241 cccg
//
LOCUS       DAOSOR010000335         6235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_12653_length_6235_cov_4.294822, whole genome shotgun sequence.
ACCESSION   DAOSOR010000335 DAOSOR010000000
VERSION     DAOSOR010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6235
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12653_length_6235_cov_4.294822"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2075
                     /locus_tag="PK710_16405"
     CDS             <1..2075
                     /locus_tag="PK710_16405"
                     /inference="COORDINATES: protein motif:HMM:NF018470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HOT11365.1"
                     /translation="WEADGADWNHRFGMYTGQTLRPAVQAKYASTMNESFVIPSKTRL
                     EQRLAAFHAAGQAGAISPEVYVRYYDTLKTYLMLTEAEHLDIAWATPRLGSEWAEYLR
                     VAPTDRGASESLIRKYLELQKAMRVAPATRDEVLTAQARGQLRNTPRILGDFERLMDT
                     IRPGTVPIGRATVFGASAANRYIVSRAGVSVDGVYTRLGWERVKKELDALEKTMAGEA
                     WVLGEGSFDPKTAVADVRKLYFEKHTIAWERFLRDLDIEKPRNVEEALPQLLALTEPE
                     WPVLRLARVVQDNVVLELTAQEAGFKSSVKDDLGAVMDAAKAIQAGDAGAVLAKPHYP
                     SPMERQFRQFLQFSIPPTPDKADAPPTPTALGQYHGVLSKLIAVLTDHKNKGADARGD
                     IKPLETEFQEAYRAVVKMLAEQDSYTRELLQNLLLKPITLSWGSVVNDAAGASGAFWE
                     ISVYRQCLTKLHSAYPFVADAQRDVSLQDFTEFFRPETGTLWSFYNQNIKTSVEKRAG
                     HFVPSQRFEQKSPYSIGFLRFLERSDEITQTFFDDGGKTPSVEFQINLHSVSPNVAEV
                     TLEIDGKPFTYKNTPEDWMVVSWPAKDAKTRGAKIRIRGLSGLVEELLNEGDFGLFRL
                     LARAKLDVGTAGGRPGEPKTIVATWFSESQQAEVKMDLKPLRSMHPFRKGFFSDVRCP
                     RLISEGGR"
     gene            2080..3048
                     /gene="tagF"
                     /locus_tag="PK710_16410"
     CDS             2080..3048
                     /gene="tagF"
                     /locus_tag="PK710_16410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagF"
                     /protein_id="HOT11366.1"
                     /translation="MFSWFNPWRRRRTALICFGKIPSDAEFLRYGTWNDAAVELQRWL
                     EKASEYAATRHKDTWKLLDSQSPLRFLFRCTQQDTALAGVVIPSSDTLGRAFPLMLAA
                     EISVRHAGRWAHTLPIALQPFFDQAAQMISRIHAASDLKDADEWLRDLQLSTLERLQD
                     HEQPYEDWACGQPLHALLHRLLGSQGESMLHTLFEATDPFRNVEAPSTNLSLLVPLQA
                     PEDLTLCFWLHVLRCAYGWKKTVPGFFAHGQSALIPLGATASPSVLIDCWLPDQPSQY
                     VCSASTDTSKYSTTLPDRIRELLDTHNATAFQLASRFESCNRSHHP"
     gene            complement(3045..5018)
                     /locus_tag="PK710_16415"
     CDS             complement(3045..5018)
                     /locus_tag="PK710_16415"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT11367.1"
                     /translation="MSRQPPHRVKGKPLRFGRYVLDHRIAVGGSSEVYLARPVNGSWP
                     ASRLVIKRLLPGVLENPHNVGTFAMEARLHRAAQHPNVVEVYEAGSVDGEPYLAMEYV
                     DGVDAYRLMRRAQNENHPIPPSVAMFMAHEVCSALACVHTAVDDTGHPLAVVHRDVTP
                     SNIYLSWDGAVKLGDFGIARLLSPESSSANTHPLKGKYAYLSPEQVAGEDFDHRADLF
                     SLAVVLSELLINQPLFPGSGQLAVLLAIRDGRIDALYDHADRLPAGLMSVLERGLARA
                     PSDRFRTAEQFRSALEPFILHDMDGCRQALGQAVKWARNTASSWKACVDPNAQRIPHR
                     FEELAAMVRTRQGETWGPSSLAKLIEWTTTGRLEAHDEVDLHGEGFEQVAAIPELARH
                     IPPSTTTTTQLHDPGAPDYRGDLPQTSMLSVLVDMLAHRETGVLFAERTQPSGLFTRK
                     EIYLAQAKVIHVASTDKKELFGEYLVRRGLIQREELDLALAVLPRYDGRLGDTLIALN
                     LVDAVQIFRAIRDQGRDRITEIFTWTSGAITFYRGVAPSRVEFPLDLELAPLMLAGLC
                     LSQPEETWLEQHQHQMDDLLVGVAEIPETMRLAAWPPEVLKVVGAAGDGRSQGDIIGS
                     LIAARLINVPSALRAIEVACAAGLLKRISARSN"
     gene            complement(5136..>6235)
                     /locus_tag="PK710_16420"
     CDS             complement(5136..>6235)
                     /locus_tag="PK710_16420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11368.1"
                     /translation="GIPRLQGYNSSMTRLVAISVAFLGVVSTACGSSDSTTDYASSPG
                     SDVGVDAAQEALPDGADEALPPDGGAVDAPQDLEPVLEGSTDTGADVLPDGQADVQEA
                     SVSDGPEPDANPSWPTCDAQPPGVADQTLPEIWSANPDSPTEVWITGLYVTAISRGKC
                     SAGAACQVFLQDALTYASFQGGAHHAMKLFVSGNTAKHFESLAVGDRVNVLAHAWRYN
                     VNPSQRELLLQVNSSLRGCAKKVGSGEPTPIEGVALTDLTVEAYENTHGPLLVKVDGV
                     SGKPGVPSETFALWKTGGPFVDAGLETVVSLSPYFLPGGVFSGLPTNATTVVSFSSIV
                     GVFGLFVPSSDGGSAKYLMLYPRTMDDVSLL"
BASE COUNT         1485 a         1811 c         1651 g         1288 t
ORIGIN      
        1 tctgggaagc cgacggagcg gattggaatc atcgcttcgg catgtacacg ggacaaacgc
       61 tacgtcccgc ggtccaggca aagtacgcgt ccaccatgaa cgaaagcttc gtgattccat
      121 caaagacgag actggaacaa cgtctggcgg catttcatgc agcgggacaa gcgggagcga
      181 tttctcccga agtgtatgtt cgttattacg atacgttgaa gacttacctc atgctcaccg
      241 aagcggagca tctggatatc gcctgggcaa caccgcgttt gggttctgaa tgggcggagt
      301 acttgcgggt ggcgcctacc gatcgagggg cttccgagtc gctgattcga aagtatctcg
      361 agttgcaaaa agcgatgcgg gtcgctccag ccacccgcga cgaggtattg accgcccagg
      421 ccagagggca gcttcgaaat acgcctcgca ttcttggaga cttcgaacgc ctcatggata
      481 ccattcgccc aggaacggtt ccgatcgggc gtgctaccgt attcggcgcc tctgcggcaa
      541 accgatacat tgtatcgcga gcaggggtga gtgtggatgg ggtgtatacg cggcttggct
      601 gggagcgagt caaaaaggaa ctcgatgctc tcgaaaaaac gatggcggga gaggcatggg
      661 tgttggggga agggtctttc gacccgaaaa cggcggtagc ggatgttcgc aaactctatt
      721 tcgaaaagca tacgatcgca tgggagcgat ttttgcggga tctggatatc gaaaagccgc
      781 gtaatgtgga ggaagcctta ccgcaattgc ttgcgttgac ggagcccgaa tggcctgtat
      841 tgcggctcgc tcgcgtcgta caagacaatg tggtgctcga actcacggcg caagaagctg
      901 ggttcaaaag ctccgtgaaa gatgacctcg gagccgtgat ggacgcggcc aaggcgatac
      961 aagcgggcga tgcgggggct gttctggcga agcctcatta tccctcccct atggaaagac
     1021 aatttcgtca gtttctgcag ttttcgatcc cacccacgcc cgacaaggcc gacgcccctc
     1081 caacgccgac tgccttgggc caataccacg gtgtgctgag caaattgatt gcggtgttga
     1141 ccgatcacaa aaacaaggga gctgatgcca gaggagatat caaacccctg gagacggagt
     1201 tccaggaggc gtatcgagcg gttgtgaaga tgctcgcgga gcaggactcc tatacgcgag
     1261 agctacttca aaaccttttg ttgaaaccga tcaccttgag ttggggcagt gtggtcaatg
     1321 atgcggcggg cgcgtccgga gcgttttggg agatcagcgt gtatcgtcag tgcctcacca
     1381 agcttcacag cgcttatccg tttgtggctg atgcacaacg cgacgtctct ctccaggact
     1441 tcactgagtt tttccgtcct gagactggca ccctttggtc cttctacaac caaaacatca
     1501 agacttcggt ggagaaacga gccggacact tcgtcccttc gcagcgcttc gaacaaaaaa
     1561 gcccttattc gataggattt ttgcgctttc tcgaacgaag tgacgaaatc acgcaaacgt
     1621 ttttcgacga tggtggcaag acgccatcgg ttgaatttca aatcaacctg cattcggtga
     1681 gcccgaatgt ggcggaagtc acgctcgaga tcgatggcaa accatttacg tataaaaaca
     1741 cgcccgaaga ctggatggtg gtgtcatggc ccgcgaagga tgcgaagaca cgaggggcga
     1801 agattcggat ccgaggtctg tcgggattgg tggaagaact actaaacgaa ggggatttcg
     1861 ggctattccg actgcttgcc cgtgcaaaac tcgatgttgg aaccgcgggg ggtagacccg
     1921 gtgaaccgaa aaccatcgtg gccacgtggt tttccgagtc acaacaagca gaagtaaaaa
     1981 tggatctgaa gcctttgcgc tccatgcatc cttttcgaaa aggtttcttc agcgatgttc
     2041 ggtgcccacg gctgatcagc gaagggggaa ggtgatcgat tgttttcctg gttcaaccca
     2101 tggcgtcgtc gtcggaccgc cttgatttgt ttcggaaaaa tcccgagtga cgcggaattt
     2161 ttgcgatacg ggacatggaa cgacgcggcg gtggagttgc aacgatggct ggaaaaggct
     2221 tcggaatacg ctgctactcg tcataaggac acgtggaaac tcctggattc ccaatcaccc
     2281 ctgcgttttt tatttcgctg cacacaacaa gataccgcat tggcaggcgt cgtgattcct
     2341 agctcggaca ccttgggacg tgcgttccct ttgatgctcg cggctgaaat ttcagttcga
     2401 cacgccggtc gctgggctca tacccttccc attgcccttc aaccattctt cgaccaagcc
     2461 gctcagatga tctcacggat tcatgcagcc tccgatctga aagatgccga tgaatggctg
     2521 cgcgacctgc agctatccac tctcgaacgc ttgcaagatc atgagcagcc ctacgaagac
     2581 tgggcttgtg gacaacccct tcacgctttg ctccatcgct tgcttggttc ccaaggggaa
     2641 agcatgttgc acaccttgtt cgaagccacc gatccattcc gaaatgtgga agcaccatcc
     2701 acgaacttga gtctgcttgt ccccctacaa gctccggaag acctcacact gtgtttttgg
     2761 ctccatgttc ttcggtgcgc ttacggttgg aaaaagacag tgcccggttt cttcgcccac
     2821 ggccaaagcg ccctcatccc cctgggcgca accgcttctc cctccgtgct gatcgattgc
     2881 tggctccctg accagcctag tcaatacgtt tgcagtgctt cgaccgatac gtcgaaatac
     2941 tccaccacgc taccggatcg tataagggag ctgttggata cccacaatgc cacggcattt
     3001 cagctagcgt ctcgattcga gagctgcaac cgcagccatc atccctagtt cgaacgagcc
     3061 gatatccgct tcagcaaccc cgccgcacag gccacttcga tggcacgcaa tgccgacgga
     3121 acattgatga gccgtgcggc gatcaacgaa ccgatgatat ccccttgcga ccgaccatct
     3181 cccgccgcgc caacgacttt gagcacttca ggaggccacg cggccaaacg catcgtctcc
     3241 ggaatctcgg caaccccaac gagcaaatca tccatttgat gttgatgctg ctcgagccat
     3301 gtttcttccg gttgcgagag gcaaaggccc gctagcatca acggagccaa ctccaagtcg
     3361 aggggaaatt ccacacgact aggggcaacg ccgcggtaaa acgtgatcgc gcccgatgtc
     3421 caggtgaata tctcagtgat tctgtctcgg ccttgatctc gaatggccct gaagatttgc
     3481 accgcgtcca cgagattcaa agcaatgaga gtgtcgccaa gacgaccgtc atacctagga
     3541 agcacggcca acgccaaatc caactcctcg cgttggatga ggccacgacg cacgagatac
     3601 tctccaaaaa gctctttttt atccgtggag gcaacgtgga tcaccttggc ttgggcaaga
     3661 tagatttctt tgcgagtgaa tagtcccgac ggctgcgtgc gttcggcaaa aagaacaccc
     3721 gtttcccgat gggccaacat gtccacgagc accgaaagca tggacgtttg tggcaaatcc
     3781 ccacgataat ccggagcccc agggtcgtgc agttgcgtgg tggtggtcgt cgagggagga
     3841 atatggcggg caagctctgg aatcgcagcc acttgctcaa aaccttcccc atgcaaatcc
     3901 acctcatcgt gggcttcgag acggcctgtc gtcgtccact cgatcaactt cgcaagagac
     3961 gacgggcccc acgtctcccc ttgtcgcgtg cgaaccatgg ccgcgagttc ctcaaagcga
     4021 tggggaatcc gctgagcgtt gggatccaca caggctttcc aggagctagc tgtgttgcgc
     4081 gcccacttca ccgcctgtcc caacgcttgc cgacatccat ccatatcgtg caaaataaag
     4141 ggctccaacg ccgatcggaa ttgctccgcc gtacgaaatc gatcgctcgg cgctcgagcc
     4201 aaccctctct cgagaacgga catcaacccc gctggcaatc gatcggcgtg gtcatacagc
     4261 gcatcgatac gaccatctcg aatggccaac aatacggcaa gctgcccact tccgggaaac
     4321 aagggttgat tgatcaaaag ctccgacaat acgaccgcca gcgaaaaaag atccgctcga
     4381 tgatcgaaat cctctcccgc tacttgctcg ggactaaggt aggcatattt ccctttgagt
     4441 ggatgagtgt tcgcggaaga tgattccgga gacaacaagc gagcgatccc gaaatcaccg
     4501 agttttactg ccccatccca actcaaataa atattcgatg gggtgacgtc gcgatgaacg
     4561 acagcgagcg gatgacccgt gtcgtccaca gccgtatgaa cacaagccag tgcggagcaa
     4621 acctcatgcg ccatgaacat cgcaacggac ggtgggattg gatgattctc gttctgtgcg
     4681 cgacgcatca atcgataagc gtcaacaccg tctacatatt ccatggcgag atagggttca
     4741 ccatcgaccg aaccggcttc atatacttcc acgacattgg ggtgttgcgc cgcgcgatgc
     4801 aagcgcgctt ccatggcaaa tgttcccaca ttatgtgggt tttcaagcac accaggaagc
     4861 agacgcttga tgacgagccg actcgcgggc cacgaaccat tcacgggccg tgcaagataa
     4921 acctcggaag acccccccac ggcgatccga tgatccaaca catatcgccc gaagcgaagc
     4981 ggttttcctt tgactcgatg gggtggctgc ctcgacatcc gtcaccgata tgctgatcac
     5041 aacacccaga tttcaaccaa ccgcgatcgg ttcatcacac atgaacccgc taattgtatc
     5101 gtattccgag cacacggcct tgcttgcatc aaccttcaaa gcaacgatac atcgtccatc
     5161 gttcgaggat acagcattag atacttcgcg ctcccaccat cgctcgaagg gacgaacagc
     5221 ccaaacactc ccacgatcga agaaaatgaa accacggtcg ttgcgttggt gggcaaacca
     5281 ctgaaaacgc ctcccggaag gaagtacggg ctgaggctca ctaccgtttc aagacccgca
     5341 tccacgaaag gacctcccgt tttccatagg gcaaacgtct cggaaggcac cccaggcttg
     5401 cccgatacac cgtcgacttt gacgagcaac ggaccatgcg tgttttcata tgcttcgacc
     5461 gtgagatcgg tcaacgccac accctcgatc ggagttggtt cacccgaacc aaccttcttc
     5521 gcacaacctc gtaacgaaga attgacttgt aacaacaact cacgctggga agggttcaca
     5581 ttataacgcc acgcatgagc tagaacgttg acgcggtccc ccacggcaag cgattcgaaa
     5641 tgcttcgcgg tattaccaga aacaaatagt ttcatggcgt gatgggcacc cccttggaaa
     5701 gacgcgtagg tgagggcatc ctgcaaaaat acctgacacg ccgctccagc actgcatttt
     5761 ccacgcgaga tggccgtcac atacaacccg gtgatccaca cttccgtggg cgaatcagga
     5821 ttggccgacc atatttccgg cagcgtctgg tccgcaaccc ccggtggctg cgcatcgcat
     5881 gtcggccaac tgggattcgc gtccggctca ggaccatccg aaacagaagc ctcctgcaca
     5941 tccgcttgcc catcaggcag aacatccgcc cctgtatccg tcgagccttc caaaacaggt
     6001 tccaagtcct gcggtgcgtc aaccgctcca ccatccggtg gcaaggcctc atcagccccg
     6061 tccggcaacg cctcttgcgc tgcatccacc ccaacgtccg aaccgggcga ggacgcatag
     6121 tctgtcgtcg aatcgctcga cccacaagcg gtcgaaacca cacccaagaa agcaaccgat
     6181 atagcaacga gtcgtgtcat ggaagaattg tagccctgca agcgcgggat gcccc
//
LOCUS       DAOSOR010000336         6218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_12696_length_6218_cov_4.779004, whole genome shotgun sequence.
ACCESSION   DAOSOR010000336 DAOSOR010000000
VERSION     DAOSOR010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12696_length_6218_cov_4.779004"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PK710_16425"
     CDS             <1..412
                     /locus_tag="PK710_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11369.1"
                     /translation="TMVKRAKAHHNSKNKARANEAGRPNLSEVLLIFAKPLLDSLPNP
                     PPTLEQVRLAMHLASIVWNLPLLAKDDPSFGNDVCASLDDVPPELGPHASKILDLMLE
                     SRNTTFADDPRFASVEVVETKEGWTVRTRSAVVP"
     gene            complement(416..1786)
                     /locus_tag="PK710_16430"
     CDS             complement(416..1786)
                     /locus_tag="PK710_16430"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOT11370.1"
                     /translation="MRIVSRILFSVSLSMLACSGPSPQTPVQEPKVEHSPPSESSPTA
                     APTTNDDPREPTASTPAESEKRGPMNVILLVIDAMRWDMPWNGYSRPIAPNLTRLAEK
                     SVRFERGYAISSYTSKSVAGLLSGRFPSEMARTSPFFTKYDDSNEMMAEVLQRRGVRT
                     VSAHAHMYLDKPSGLTQGFDVWRLISGIQFDYNKDPYITSPQYTDLVKSILEAPENTS
                     GRFFGYFHYMDPHHEYNTHKEAPTWGTTSRDLYDEEIWFTDKWVGELLTFIESRPWAK
                     NTAIIVTGDHGEGFGERVGGVDNYRIWKHAFELYEVLIKVPLFVYVPGVEARQIARWR
                     GHIDLVPTIFDLLGEQPPEGLSGVSLVPEIKGEDVPPRPIIVDLPADTHNRRRRALIE
                     DGYKLIAFGKDFRFTLYNVKDDPGESKELSRVEPERKKAMVERYRGIVAGIHDVKAVG
                     APVLDD"
     gene            complement(1884..2981)
                     /locus_tag="PK710_16435"
     CDS             complement(1884..2981)
                     /locus_tag="PK710_16435"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOT11371.1"
                     /translation="MLRRIVIGWLLIGCDRPVALAEPGVSISSASDTLAPLVVTPPAS
                     ASHAGTSASPAAARPCIPSEYEEVPLVLTPGQVRQALVNNNELELRNAVKMLGGPSEE
                     PSSRSVQRIAFFQKAVQAYASGISVHGIVDPPTLRALERAVPSLHEALQPSTPPACWL
                     RWPGSTAEQRYFMMRVYDVQRWRSAQKRMPAPIPDSLAPIEDDRTAQVDAAKACRELL
                     REARRDKPRHGNLQVAYGYRSALTQLEIWEYHFPARYRETSSQRSTFPQGPHGPQAIL
                     HLATVYASRTASPGFSLHQRGTAIDFVCTTDDGSVLKPTGRFLSSWKRSWCLRWLEQH
                     ARTFGFMPNATIDEPWHWEYHKPCEKKPCDE"
     gene            3093..4133
                     /locus_tag="PK710_16440"
     CDS             3093..4133
                     /locus_tag="PK710_16440"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOT11372.1"
                     /translation="MIALEKLDIVDEAHDRALLDLWIHDFGACVFSAADEVGIFVALA
                     QGSQTVAQLADRLRLEPSALLAVCRALVSLQGLVESNDTFDLSAFSQAVWVKQTPCYR
                     GREFDRHRDWEQHQRIVDTLEGRWAPIDDGQRPFSEGWRDGDVDPQAAYRFTRVMHSM
                     IFLPSLAAARSGALSEVRHLLDVGGGSGIFSAALIAHQPEVSACVLDLPQVCEASRRI
                     LRPYETAARVKYHPCNFFHDPWPAEVDGVWLSNVLHDWPVETCKTLLKKAYHSLPIGG
                     RIVIHEALLDACRCSPRFTTLFHLLMLMNHRGQQFTKEDLFGLLLDCGFTQPRVVFGY
                     SVWSVIEGRKTI"
     gene            complement(4157..>6218)
                     /locus_tag="PK710_16445"
     CDS             complement(4157..>6218)
                     /locus_tag="PK710_16445"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HOT11373.1"
                     /translation="HLDVQATPVVVNFNQPPVSGPPSIIAPFTATVPGSYTEGLGVLR
                     ILRGTDCTVEAVLGGTDLDGDGIVDWVASPATPALGDLDGDGVADIVTYGGDGSLLAF
                     TRKSGVWSLLWKGRDSAGGNVFIDPIYSWTPDRSANWAGPSIHDLDDDGVPEIIREGY
                     VFSAEGILLSGLPPGYAPASSGLNPVLANLDDDPDIEFTNGQFIWAWSNGQWVQEGTF
                     PGASPSAPGYVAVADFGPYGSGSPTTPEIVVVRSSYAMIYATDGTFAMDPIAIPGGGG
                     GNPTVADYDGDGLPELGVAGADYYTVFDIDCTATPRAGGQCASTGQQRCDHVAGSECP
                     AGILWSRKTQDHSSNITGSSVFDFEADGKAEVVYADECFVRVYDGTTGDVLFSQYRSS
                     CTWFENPVVADVDGNFRADLVVPSNLACAQGGAGVACGDLNEDGVDPQFAGLRCKEAT
                     DCVSGSCVDGLCRCTSTSQCCSSQDDALCLEMGFACVNPPASIGGEKTCRAAHPHGVT
                     GIRVYSDSQDRWVRSRTVWNQHAYAVTHVHDDGTIPATSAWVKNWEHPDLNNFRQNVP
                     GTPNGQSTADPTAGASQFNACGGGKAMLNVAICNRGADAMGAGVSVGFYDGSELVCET
                     KTSSALQPGECETVTCTWDNPPTSSTSAKDIKVVANYDGALRECKEGNNEGIITGVYC
                     AKPR"
BASE COUNT         1393 a         1761 c         1703 g         1361 t
ORIGIN      
        1 caccatggtc aaacgtgcaa aggcccatca caattcgaaa aacaaggctc gtgcgaacga
       61 ggctggccga cccaacctgt cggaagtcct gctgatattt gcgaaacctt tgctcgatag
      121 ccttcccaat cctcccccca ccctcgagca agtacgcctc gccatgcacc ttgcctcgat
      181 cgtatggaac ctccccttgc tcgccaaaga cgacccttct ttcggcaatg atgtctgcgc
      241 ttccctggat gacgtgcccc ccgaactcgg tccccacgct tcaaaaatcc ttgatttgat
      301 gctggaatcg cgaaacacca ccttcgccga cgatccaagg ttcgccagcg tggaggttgt
      361 cgaaaccaaa gagggatgga ccgtgcgcac ccgaagtgcc gtcgtcccat gacgctcagt
      421 cgtcgagcac gggcgctccc accgccttga cgtcgtgaat gccggcaacg atgccgcgat
      481 aacgctccac catcgccttt ttacgctctg gttccacccg agacaattcc ttggactcgc
      541 ccggatcgtc tttcacatta tacaatgtga agcggaaatc ctttccaaaa gcaatgagct
      601 tgtagccatc ctcgatgagg gcgcggcgac gtcgattgtg ggtatcggcg ggaagatcga
      661 cgatgatggg ccttggggga acgtcttcgc ccttgatttc gggaacgaga ctcactcccg
      721 aaagcccttc gggaggctgt tcaccaagca agtcgaagat ggtgggaacc agatcgatat
      781 gtcctcgcca tcgcgcgatt tgccttgctt cgacgccggg cacgtagacg aacagcggta
      841 ctttaatcag cacttcgtaa agctcgaaag catgtttcca aatccggtag ttgtcgactc
      901 cgccgactcg ctccccaaac ccttccccat gatctcccgt cacgatgatg gccgtgttct
      961 ttgcccacgg tcgggattcg atgaacgtga gcaattcgcc gacccactta tccgtaaacc
     1021 aaatctcctc atcgtataaa tcacgggatg tcgttcccca cgtcggcgct tccttatgcg
     1081 tgttgtactc gtggtgagga tccatgtagt ggaagtagcc gaaaaaacgc ccagatgtgt
     1141 tttccggtgc ctctaggatg cttttcacca gatcggtata ttgtggcgac gtgatgtatg
     1201 ggtctttgtt gtaatcgaat tggatacccg agatcagacg ccacacatcg aagccctgcg
     1261 taagaccact cggtttgtcc agatacatat gcgcgtgcgc gctgacggtt cgcacaccac
     1321 ggcgctgcag cacttccgcc atcatctcgt tggaatcgtc gtacttggtg aaaaacggag
     1381 aggttcgcgc catttccgaa ggaaaacgcc cgctgagaag ccccgcgacg ctcttggacg
     1441 tatacgatga aatggcataa ccgcgctcga aacggacaga tttttccgca agacgagtga
     1501 ggttgggagc gatgggacgg gaataaccgt tccaaggcat gtcccatcgc atcgcatcga
     1561 tgacgagcaa aatgacgttc atcggcccac gcttttcgga ttcggctggc gtgctagccg
     1621 ttggctctcg gggatcgtca ttggtcgtcg gtgcagcagt gggggaagat tcgcttggag
     1681 gcgaatgctc aaccttgggt tcctgaaccg gagtctgggg tgagggtcca ctgcatgcga
     1741 gcatggatag agatacggaa aagaggatcc gggatacgat tcgcatggcg tgcaagattg
     1801 tcgctcaatc ggtgaaacgc aacccattcg tgtgatcgac gacagcgtat ccacgaatta
     1861 ccatgggcac tcgatgagcc aacttactca tcacaaggct ttttttcgca aggtttgtga
     1921 tattcccaat gccacggttc gtcgatggtg gcgtttggca tgaagccaaa agtgcgagca
     1981 tgctgctcga gccaacgcaa acaccaagac cgcttccatg aggaaagaaa tcgacctgtc
     2041 ggtttcaaca cggagccatc gtccgtggtg caaacaaagt cgatcgctgt tcctcgttga
     2101 tggagactga agccaggaga agccgttcgt gatgcgtaaa cggtagcgag atgcaatatg
     2161 gcttgcgggc catggggtcc ttgcggaaac gtcgatcgct gactcgatgt ttcccgataa
     2221 cgtgccggaa aatgatactc ccagatctcg agttgggtca gcgcggagcg atagccgtaa
     2281 gccacctgaa ggttaccgtg ccgaggcttg tcacgacgag cctcgcgcag aagctcacga
     2341 catgccttgg cagcatcaac ttgcgccgtg cgatcgtcct cgatgggagc caacgaatcg
     2401 ggaatggggg caggcatacg tttttgtgcg gaacgccagc gctgcacgtc gtacacacgc
     2461 atcatgaaat atcgctgctc cgcggtggag ccgggccagc gaagccagca tgccggagga
     2521 gtggatggct gcaaggcttc gtgtaacgaa ggaaccgccc tttccaaagc tcgaagcgtg
     2581 ggaggatcga cgatgccatg cacggaaatg cccgaagcgt acgcttgcac ggctttttga
     2641 aagaaagcga ttctttgcac cgagcgagat gatggttctt cggacggacc tccaagcatc
     2701 ttgacggcat ttcgtagctc gagttcgttg ttgtttacga gtgcttgccg cacttggcct
     2761 ggtgtgagca ccaggggaac ctcttcgtat tcagacggaa tacaaggtcg tgcagcggca
     2821 ggagaagcag atgtgccggc gtgcgaggcc gaggctggcg gtgtcaccac gagcggtgcg
     2881 agagtgtcag aggcgctgga aatagagacg cctggttccg caagggccac aggacgatca
     2941 cagccgatga gcagccatcc aatcacgatt cgccgcaaca caatgcgttt tcgtatacca
     3001 cggggcgtcg aaaacagtga tcgagcggtt catgaaagga aaggaacgac gaaatcgagt
     3061 catcgttgtc gttctatgat acctccgagc tgatgattgc tctcgagaag cttgatatcg
     3121 tggatgaggc gcatgatcgg gcgcttctcg acctgtggat ccatgatttt ggagcttgtg
     3181 tattcagtgc ggccgacgag gttggaatct ttgtggctct ggcacaaggc tcccaaaccg
     3241 ttgcgcaact cgccgatcgc ctgcgtctcg aaccatcggc gcttcttgct gtatgccgag
     3301 cgctcgtttc gttgcagggc ctggtcgaat ccaacgatac gttcgatctc agtgcttttt
     3361 cccaggcagt ttgggtcaaa caaacacctt gctaccgagg tcgtgaattc gatcgacacc
     3421 gtgattggga acaacaccaa cgaatcgtcg atacgctcga aggccgttgg gcacccatcg
     3481 acgatgggca gcgaccgttt tcggaagggt ggcgtgatgg tgacgtggat ccgcaagcgg
     3541 cttatcgatt cacccgtgtc atgcactcca tgatttttct tccttctttg gcagcggccc
     3601 gaagcggtgc tctatcagag gttcgtcatc tgctggatgt gggaggaggg tcgggaatct
     3661 tcagcgcggc actgatcgcg caccagcccg aggtgtctgc ctgcgttctc gatcttccgc
     3721 aagtctgtga agcttcacgt cgcatccttc gaccttatga aaccgcagca cgcgtgaagt
     3781 accatccatg caattttttt catgatccgt ggccggcaga agtcgatggt gtgtggttgt
     3841 cgaatgtgtt gcatgactgg cccgtggaaa cgtgcaaaac gcttctaaaa aaggcatatc
     3901 attcacttcc gatcggtggg cgcatcgtga ttcatgaagc acttctcgac gcttgtcgct
     3961 gctctccccg attcactacg ctgtttcact tattgatgct gatgaaccat cgcggacaac
     4021 agttcaccaa agaagatcta ttcggcttgc tgctggactg cgggttcacg caaccacgcg
     4081 ttgtttttgg ctattccgtt tggtcggtca tcgaggggcg aaagacgatc tgacgatagt
     4141 ggttcgtgcc attcgatcag cgaggcttgg cacaatacac cccggtgatg atcccctcgt
     4201 tattgccttc tttgcactcg cgcaaagcac cgtcatagtt ggctacgact ttgatgtctt
     4261 tggccgatgt agacgacgtc ggagggttgt cccaggtaca agtgacggtt tcgcattcgc
     4321 caggttgaag ggctgaggat gttttggttt cacaaacgag ttcggaacca tcataaaaac
     4381 caacggatac accagctccc atcgcatcgg cgccccgatt gcaaatcgcg acattgagca
     4441 tggctttgcc tccaccacaa gcgttgaatt ggctcgcgcc cgccgtggga tccgcggtgg
     4501 attgaccgtt gggtgttccc ggcacgttct gccggaaatt attcaaatcc ggatgttccc
     4561 aattcttgac ccatgcggat gtggcaggaa tcgttccgtc gtcatgcaca tgggtgaccg
     4621 cataagcatg ctggttccac accgtgcgcg atctcaccca cctgtcctga ctatcggaat
     4681 atacgcgaat gcccgtgaca ccatgaggat gagccgcacg acacgttttt tctccgccaa
     4741 tcgaagccgg tggattgaca caggcaaaac ccatttcgag acaaagcgca tcgtcttggg
     4801 aagaacaaca ctgggacgtg gatgtgcatc gacacaaacc atcgacgcat gaccccgaaa
     4861 cgcaatccgt cgcttctttg catcggagtc ccgcaaattg cggatcgact ccatcctcat
     4921 tcaaatcacc gcaagccact cccgctcccc cctgcgcaca agccaggttg gaaggcacga
     4981 cgagatccgc tcggaaattg ccgtctacat cggcaaccac agggttttca aaccatgtgc
     5041 atgaggaacg gtattggctg aacaatacgt ctcccgtggt gccgtcatac acgcgaacga
     5101 aacactcgtc ggcatatacg acttctgctt tcccatcggc ttcgaaatcg aataccgatg
     5161 aacccgtgat gttggaagag tgatcctgcg tcttgcgaga ccataaaata ccggcgggac
     5221 attcgctacc ggctacgtga tcgcatcgct gctgaccggt ggatgcgcat tgtccacccg
     5281 ccctcggcgt tgccgtgcaa tcgatatcga agacggtgta ataatccgct cccgccacgc
     5341 ccaactccgg aagcccatcg ccatcatagt cggctaccgt gggattgcct ccgcccccac
     5401 cagggatcgc aatcggatcc atcgcaaacg tgccatcggt tgcgtaaatc atcgcatagc
     5461 tgcttcgtac caccacgatc tccggtgtcg tgggtgaacc agaaccatac ggaccaaaat
     5521 ccgcaaccgc tacatatcca ggcgctgagg gactcgcgcc aggaaatgtc ccttcctgta
     5581 cccattgacc gttggaccac gcccaaatga attggccatt cgtaaactcg atgtccggat
     5641 cgtcgtcgag attggcaagc accggattga ggcctgatga tgcaggcgca tatcccgggg
     5701 gaagaccgga cagtagaatc ccttccgccg agaaaacata accttcacgg atgatctcag
     5761 gaacaccatc gtcatccaag tcgtggatcg acggccccgc ccaattggcc gaacgatccg
     5821 gtgtccacga gtatatcggg tcgatgaata cgttaccgcc agcggagtcc cgtcctttcc
     5881 aaagcaaaga ccatacgccg gattttcgcg taaatgcgag caacgaacca tctccgccgt
     5941 aggtcacgat gtccgccacg ccatcgccat ccaggtctcc caaggctggt gtggccggcg
     6001 atgcaaccca gtccacgatg ccatcgccat cgagatcggt gcctcccaac accgcttcga
     6061 ccgtgcagtc ggttccacgt aaaatgcgaa gcacgccgag tccttccgta tacgagccag
     6121 gcaccgtggc cgtgaaagga gcaatgatgc tgggcggacc actgacaggc ggctggttga
     6181 agttgacgac aaccggggta gcctgcacat cgagatgg
//
LOCUS       DAOSOR010000337         6181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_12787_length_6181_cov_3.572804, whole genome shotgun sequence.
ACCESSION   DAOSOR010000337 DAOSOR010000000
VERSION     DAOSOR010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12787_length_6181_cov_3.572804"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="PK710_16450"
     CDS             complement(<1..913)
                     /locus_tag="PK710_16450"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11374.1"
                     /translation="MIFFDLLPPRLRRCRPVALAALSGVGVALAAPGVLSVAPPWAMA
                     WLAWIALVPLLVGISESSPPKAAFLGWVASMMLQVIAMSWFPGLMARFAGLPPGLAIG
                     LGLTIFALQALGWAIWAASLRYLFPRWPLSVAAPLTFMAMEYAMPLLFPFSLGLTQYC
                     NLPLAQVAEAGGIHVVSALLVAFNAAVAHGIVSKTRRWQTAILALSMMVLIHGGGWGR
                     MVQIRNRRQSAAPMRMGWVQAGVIAKGWQAGAEQINLTDYQHLSHILENDVGPLDLVI
                     WPEKAVGLLLRTARHDYGLSHPLRIRKG"
     gene            1127..2245
                     /locus_tag="PK710_16455"
     CDS             1127..2245
                     /locus_tag="PK710_16455"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT11375.1"
                     /translation="MSPAPSDIHLTLSLTHDCNLRCHYCYSGAKQARHMPLEVGKRAL
                     QSALRQTRRRLHLVFFGGEPLLRWADLLALTDEARTRGESCGVQILPTLTTNGTLLTD
                     DRCAELAQRRFLLAVSCDGLEMAHDALRRDRAGMGSFARTWQGINTALKAHLRPRIAL
                     VVHPSNIAYLPESIEFFVAAGLRDFVVNLDWSAPWDARGMKERLTHAYENVAALYVQA
                     YRDANPFWISMIDSRIADRIRGVDTQDRCDLGRRNLVVAPSGNLYPCDRLIGEDDGLM
                     SIGHIDSGVDARRLEAFMKQASFIPTACLSCAIRERCRHRCACANLAMSGHAGTPTSL
                     LCFYEQLCLRTADDAAEMLFSERNEGFLGQHYGDKVPF"
     gene            2296..2544
                     /locus_tag="PK710_16460"
     CDS             2296..2544
                     /locus_tag="PK710_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11376.1"
                     /translation="MGSKLSPVSAYRERAYPKVESPTLQRLLLLVGMTATSLGGCGPA
                     IDSRTVDRAIDEGDLDGAVDCDANPEAESCTPADASTE"
     gene            2562..3557
                     /locus_tag="PK710_16465"
     CDS             2562..3557
                     /locus_tag="PK710_16465"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HOT11377.1"
                     /translation="MRVFWLLLGLSVLVLWHEMGHALVARALRLHLTDFSFGFGPTMA
                     SRSWRGVRWRWGMIPFGGFVRVAQLSLRADEPGRFLPRAVLSRLLTIVAGSAANLLLA
                     AILAFSSAWWWGEPTGAISGLQVTMVPERSALRVGDRIEKINDIEVKSVHDLRRAVAK
                     AKIAEVQLWRAGNLMHLPIVPIERAGKRGFDARYVIVEQTYKPNLRQALGIGARAPYH
                     QAISLFRNSGTIVRPDSGVRPVTAVGLADRVSQTGRWDGRRLFSLASTLSVAVGLFNL
                     LPIPGLDGARFVLEVVESVRRKRISIRWALYVQVAGAVFLGLLWLWLGWLDCLGR"
     gene            3768..4334
                     /locus_tag="PK710_16470"
     CDS             3768..4334
                     /locus_tag="PK710_16470"
                     /inference="COORDINATES: protein motif:HMM:NF023043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HOT11378.1"
                     /translation="MNLGFRPYVLLGCAFSMVLAVSGCCKDKTQDTPGETPAVASETP
                     VIPGLPTKKPVSVKTIKIGESAEMSEFKLEAKEAKECKAKGYSRPKKDHKWVGVDVLI
                     EATGTKELLVNQLSAKVTDSEGVVHNASYATQSSCGEHFKSARLNNGEKAKGWLIFEV
                     PKAAKGFTMTYTERSYTESDAVKVELGL"
     gene            4496..5005
                     /locus_tag="PK710_16475"
     CDS             4496..5005
                     /locus_tag="PK710_16475"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOT11379.1"
                     /translation="MIDVQGKLAQIVENSETLHINISKLIRGFQALEVPILWLEQIPE
                     KLGPTTKSLAELFKNQRPIAKTTFSAVRNNDFMDRLQRSKRRKLLLAGIETHICVYMT
                     ASELVANNYYVEVIADAVSSRNPKNHAIGIAKMQNQGVSVTCVETVLYELLGDARSEH
                     FKNILEIVK"
     gene            5077..5943
                     /locus_tag="PK710_16480"
     CDS             5077..5943
                     /locus_tag="PK710_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT11380.1"
                     /translation="MVESRKPPATYCSLFQTYARMGAVFVASTLPIHACQSNKDSLPP
                     SPPVSASAFASTSASAVSLPTAAASDGAADGDVRRVLVEQLEIDGDLPAFVVRGKKDG
                     FKMVFLHGLCGNPYAYALSFRHAAARLGTLLALQGNISCGSDFRDWSCPPSQVDARIE
                     RAFRALGDESDLRNLILIGYSSGATYAELLVLRNPSRYALPILIASPQKPAYFRLRKA
                     RGVVLMAGERDRHDLMKSGLRDLSAREIPSAFFILPGATHGEMGNDAENVMENALLWI
                     VRNALADSTTAP"
BASE COUNT         1401 a         1641 c         1695 g         1444 t
ORIGIN      
        1 atccttttcg gatccttaag ggatgggaaa ggccatagtc atgacgggcc gtacgaagca
       61 gcaaccctac cgctttttcc ggccatatca ccaagtccag cgggccgacg tcgttttcca
      121 ggatatggct aaggtgttga taatccgtca aattaatctg ctccgctccg gcttgccacc
      181 ctttcgctat cacgccagcc tgtacccaac ccatccgcat cggagccgca gattgccgtc
      241 gattccgtat ttggaccatc cgtccccagc cccccccatg aatcaatacc atcatggata
      301 acgccaggat cgccgtttgc caccgccgtg ttttcgagac tatcccatgg gcaaccgcag
      361 cattgaacgc cacaagcaag gcacttacga catgaatccc ccctgcttcg gcaacctgcg
      421 caagaggaag attacaatat tgcgtcaggc cgaggctgaa agggaatagc agaggcatgg
      481 catattccat cgccatgaag gtaagggggg cggccacgga aaggggccaa cgcgggaata
      541 agtagcgaag gctcgcggcc cagatcgccc atcccaacgc ttgcagcgcg aaaatcgtaa
      601 gtccgaggcc gatggccaag ccgggaggaa ggccagcaaa acgggccatg agccccggaa
      661 accaagacat ggcgatgact tgaagcatca tgcttgccac ccaacccaag aacgccgcct
      721 tgggtgggct gctttccgaa atccccacga gcaatggcac gagcgctatc cacgctaacc
      781 atgccatcgc ccatggcgga gccacgctca aaacgccagg cgccgcaagg gcaacaccca
      841 ccccggatag cgccgcaaga gcaaccggac ggcaacgacg caaacgaggt ggcagcaaat
      901 caaaaaaaat catggtgaaa tcgacgggtc aagccttctg aggctccgct cacgagacgt
      961 tccgcgaaca tgccgcccat gagacagcaa caggcgcgat gggtgaaggc gtgcccagga
     1021 tacgcgcctt gcagggactg ttcgaaagga catctgcgga cttggttttg ggcaacgacg
     1081 ccaacgacgc ctttcgttcg acaccaaaga gcatcatact cgaagcgtgt ctcctgcgcc
     1141 gagcgatatt cacctgacgc tatcgttgac ccacgattgc aatcttcgtt gccattattg
     1201 ctactcaggt gcgaaacagg cacgacacat gccgctggaa gtgggaaaac gtgcgttgca
     1261 atccgcgttg cggcagaccc ggcgacgatt gcatctcgtt ttttttggag gggaaccttt
     1321 gctacgctgg gctgatctgt tggccttgac cgatgaggcc cggacaaggg gagaaagctg
     1381 cggcgtgcaa attctcccga cccttaccac caatggtacc ctgctgacgg acgatcgctg
     1441 cgccgaactc gctcaacgtc gctttctact ggccgtatct tgcgatgggc tcgagatggc
     1501 ccacgatgct cttcgacgcg atcgcgccgg aatgggtagt ttcgctcgga cctggcaagg
     1561 aatcaacacc gccctgaaag ctcatctacg gcctcggatc gcgctcgtgg tccatccttc
     1621 gaatattgcc tatctgcctg aatctattga gtttttcgtt gccgcgggcc tgcgggactt
     1681 cgtggtcaat ctggattggt ccgccccctg ggatgctcga ggaatgaagg agcggctcac
     1741 ccacgcttac gaaaatgtgg ctgccttgta tgtgcaggct taccgcgacg cgaatccctt
     1801 ttggatatcg atgatcgact cgcgcattgc cgaccgtatc cgaggggtcg atacgcagga
     1861 tcgatgcgac ctgggccgac gaaatctcgt cgtggctccc agcggcaacc tttacccttg
     1921 tgaccgattg ataggcgaag acgacggcct tatgtcgatc ggtcatatcg actccggggt
     1981 ggatgcaaga aggctcgaag cattcatgaa acaggcttct ttcattccga ccgcgtgttt
     2041 gtcttgcgcc atccgcgagc ggtgtagaca tcgatgcgcc tgtgccaacc tcgcgatgag
     2101 cggccatgcg ggaacaccta cttccttgct ctgtttctat gaacaacttt gccttcggac
     2161 ggctgacgac gcggccgaga tgttgttttc ggaaaggaac gaaggattcc ttggacagca
     2221 ttatggtgac aaggtacctt tctaatgctt atcgcttgtt tcgcacggtt ctgagttcga
     2281 ccgaaggagt tgatgatggg ttcgaaatta tcgcccgttt ctgcttatcg cgaacgtgct
     2341 taccccaagg tcgagagccc cacgctgcaa cggttgttgc tgctcgtggg gatgaccgcc
     2401 acttcgcttg gaggatgcgg ccccgcgatc gattcgcgta ccgtcgaccg cgccatcgat
     2461 gaaggcgatc tggacggtgc ggtggattgt gatgcgaatc ccgaagccga atcttgtacc
     2521 cccgcggacg cctcgaccga gtgagcgtcg agacatcttg aatgcgtgtg ttttggctac
     2581 tactcggcct cagcgtcctg gtgttgtggc acgaaatggg gcacgcgctg gtcgccaggg
     2641 cgttgcgtct tcacctaacc gatttttcct ttggctttgg ccccacgatg gcttcgagat
     2701 catggcgggg cgtgcgctgg cgatggggaa tgatcccttt cggcggtttc gttcgtgtcg
     2761 ctcaactttc gttgcgagcc gatgaacccg ggcgatttct tcctcgtgcg gttctttccc
     2821 gactcttgac cattgtggct ggctcggccg ccaatctgct gttggcggca atactcgctt
     2881 tttcgagcgc gtggtggtgg ggcgaaccca cgggggcgat aagcggtttg caagtgacga
     2941 tggttcccga acggtccgca cttcgggtgg gggaccgaat cgagaagatc aacgatatcg
     3001 aggtgaaaag cgtgcatgat cttcgtcgtg cggtagcaaa agccaaaatc gcggaagtcc
     3061 aattatggcg ggcaggcaac ctcatgcatt tgcccatcgt acccatcgaa cgggctggta
     3121 agaggggatt cgatgctcgt tatgtcattg tcgaacagac ctataaaccg aacctgcgcc
     3181 aagcgttggg gataggcgca cgagcgccat accatcaggc catctcgctt tttcgaaaca
     3241 gtgggacgat cgtcagacca gattccggcg tgcgcccggt aacggctgtc ggattggccg
     3301 atcgggtgtc gcaaaccgga cggtgggatg gtcgacgcct cttttctttg gcctcgacgc
     3361 tttcggtggc ggtggggctg ttcaacttat taccaatacc gggcttggat ggggcccgct
     3421 tcgtgctgga agtcgtggag tccgtccgac gaaagcgcat ttcgattcgt tgggccttgt
     3481 atgtgcaggt ggcgggtgcc gtttttttgg ggctgctgtg gctatggcta ggatggctcg
     3541 actgcctcgg acgataaggg gttgagcggg ctcatctttc ttgccaaaat ggtattattg
     3601 tgaaaattgc agaaaataat acgaatgcaa agaccaaaac ctaagacagt ctcctcggat
     3661 gtggtgtaac tattggaatt cattattaaa attactattt cctgaaagag tgatcgcttt
     3721 ctggaacggc atattgtacc ttcagctccg aaaaaaggag aggattcatg aaccttggat
     3781 ttcgaccgta tgtccttctg ggttgtgcat tttcgatggt tcttgccgtg tcgggatgct
     3841 gcaaggacaa aacgcaggac acgccagggg agacacctgc tgtcgcttcg gaaacgccgg
     3901 tcattcctgg cctgcccacc aaaaagcccg tctcggtaaa aacgatcaag atcggggagt
     3961 ctgcggaaat gagcgaattc aaactcgagg caaaagaagc caaagagtgc aaggccaaag
     4021 gatattcgcg ccccaaaaag gatcataagt gggttggcgt agacgttttg attgaagcca
     4081 ccggcaccaa agagcttctg gtcaatcaat tatctgctaa agtaacggat tccgaaggcg
     4141 tggttcataa cgcatcctat gcaacacaga gtagttgtgg agaacatttt aaatcggcac
     4201 gcctcaacaa tggtgaaaaa gcaaaaggat ggttgatttt tgaagtaccg aaagcggcga
     4261 aagggttcac catgacttat actgagcgaa gttatacgga gagcgacgcc gttaaagtgg
     4321 aattaggctt gtagcgattg tctgcaccct gctgtcttgc agaacccaat cgatggtatt
     4381 ttccgtcgat cgactgatct ccaattctat tcaatctcct gaacctttcg actataatag
     4441 tatctgaacg aaatgagtaa ttgaaatgct ccatgtcgag gatacagcgc tcgtcttgat
     4501 cgatgtgcag gggaagctgg cgcaaatcgt cgagaattcg gaaacgcttc atatcaacat
     4561 ctcgaagctg attcgaggct ttcaagctct tgaagtcccc atcctgtggt tggaacaaat
     4621 accggaaaaa cttggtccaa cgacgaaatc tctcgccgag ttgttcaaaa atcaacggcc
     4681 gatcgcaaaa actaccttta gtgcggttcg aaacaatgac ttcatggata gactacaacg
     4741 gtcgaaacga agaaaactac tgcttgcggg aatagaaact catatctgtg tatatatgac
     4801 ggcctctgaa ttggtggcca ataactacta tgtggaagtg attgcggacg ctgtttcgtc
     4861 caggaatccg aaaaaccatg cgattggtat tgcaaaaatg caaaaccaag gcgtgtccgt
     4921 gacctgtgtc gaaactgtat tatatgaact gctgggtgat gctcgctcgg aacatttcaa
     4981 aaatatcctc gaaatcgtaa aatagcgata ttaatatgga agagagcggg ccaacgtcga
     5041 caagtcaaaa gtaattaatc attcgggaga tatctcgtgg tcgaatcgag aaagcccccc
     5101 gctacgtatt gcagtctgtt tcagacttat gcgcgcatgg gggctgtttt cgttgcatcg
     5161 acgttgccca tccatgcatg ccagagcaat aaggactccc tcccgccgtc gccccctgtt
     5221 tctgcctccg ctttcgcctc cacctctgcc tctgcggtgt ccctgcccac cgcggcagca
     5281 tccgatggcg cagccgatgg ggacgttcgg cgagttctgg tggagcagct cgaaatcgat
     5341 ggcgatcttc cagcttttgt ggttcgtggc aaaaaagacg gtttcaagat ggtgtttttg
     5401 catggtttat gcggaaaccc ttatgcctat gccctctcct ttcgccatgc cgccgctcgc
     5461 ttggggactc tgcttgccct gcaaggcaat atttcatgcg gttcggactt tcgagattgg
     5521 tcctgtccgc caagtcaggt ggacgccagg atcgaaagag cttttcgtgc gctgggtgat
     5581 gagtcggacc ttcgtaacct tatccttatc gggtattcgt cgggggccac gtatgccgag
     5641 ctgcttgtct tgcgaaatcc atctcgctat gctctgccca tcctcatcgc aagtcctcaa
     5701 aaacctgcgt attttcgcct tcgcaaggct cgtggggtgg tcttgatggc aggagaacgc
     5761 gacaggcacg acctgatgaa gtccgggctt cgggatttat ccgcaaggga gattccatct
     5821 gcctttttca ttcttcctgg cgcaacccac ggtgaaatgg gtaacgatgc cgagaatgtg
     5881 atggaaaatg ctttgctatg gatcgttcga aacgccttgg ccgactcgac caccgctccg
     5941 tgatcttccg gctcccataa tttcaatcat gcgtgattag aaggcgaagg atggcatgcc
     6001 atggtcagaa ctttttgacg gggccaccca cggcttcttg aaaaggaatc tctttggaaa
     6061 tatttcgata ccgttccatc atcgccgcgg cacgctcttt ttcaacttcg atgagatttt
     6121 tgctctcatg cggatcatct ttgatattgt acatctcgaa aactcgatcc gcgccggcca
     6181 c
//
LOCUS       DAOSOR010000338         6169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_12817_length_6169_cov_3.916421, whole genome shotgun sequence.
ACCESSION   DAOSOR010000338 DAOSOR010000000
VERSION     DAOSOR010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12817_length_6169_cov_3.916421"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PK710_16485"
     CDS             complement(<1..448)
                     /locus_tag="PK710_16485"
                     /inference="COORDINATES: protein motif:HMM:NF026057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HOT11381.1"
                     /translation="MDDIQAREWAFLQFGTAKLGDLRNDKRLLKIAATVVRGGGGTIS
                     QGMTDPREREAAYDFVENERISAEAVGHAAAVAAARQIAREPFVWLAVDGSSSSLPDS
                     RKTKGMGRLGSGHKQGTGIKVITCLAVDPQGVPVGPGEFRWWVRSGK"
     gene            complement(600..1541)
                     /locus_tag="PK710_16490"
     CDS             complement(600..1541)
                     /locus_tag="PK710_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOT11382.1"
                     /translation="MMPLPAPRLCAVSVDLDEITNYFDIHGLPPPSGDLAHVVYQVAL
                     ERLEAFAASQNIPLTLFVIASDLAHSKNSQILQRLASRGHELANHSFDHFYDLTRRSK
                     EEMKAQVIDAAEAIERHTGVRPGGFRAPGYLITDELVDVLREAGVKYDSSVFPCPAYH
                     AAKALAMGRIRFQGRESRSILDHPRTLRAPTRPYRLGRPFRCRGQGPVELPIQVTRGL
                     RLPYIGTSLTLAGEKGALWLTRMVIGEPLVNLELHGIDWLDRFDGLSALEGHQPDIRI
                     SLDRKLLIFAGVIQRLRASGYAFVRLDEAAEAFGARG"
     gene            complement(1551..2480)
                     /gene="tsf"
                     /locus_tag="PK710_16495"
     CDS             complement(1551..2480)
                     /gene="tsf"
                     /locus_tag="PK710_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOT11383.1"
                     /translation="MASVTPQLIRELRERTAAGMTDCKNALVEAEGDIDKAVEIILKK
                     GKAKSAKRATATATEGEIRANMATDGRAGTLVEINIQTDFAARNDKFKAFVDEVAQLV
                     GNVNNLDELLASKMAAGKTVAEIRDDLIATIGEKIDVRRMQRLTLDAPAGNVHSYVHM
                     NGKIGVLLATAAENDAVAKHEAYLKFVDDTAMQIAAMSPLYLDRNQVPAADIEKQKEI
                     FAEQLKQEGKPEVAWPKIIEGKVNKWYTELCVVDQDSVIENGKSVDQVRAEAAQQAGG
                     SITLLGFVRFQLGEGLSRAEEDFAEEAKKMAGS"
     gene            complement(2561..3409)
                     /gene="rpsB"
                     /locus_tag="PK710_16500"
     CDS             complement(2561..3409)
                     /gene="rpsB"
                     /locus_tag="PK710_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOT11384.1"
                     /translation="MPSFAPRAPGSPALSVRALLESGVHFGHQTKRWNPKMRPYIYGA
                     RNGTHIIDLDQTAKLFTRAYDFIQEAVSRGGSVLFVGTKRQAQDIVREEALRAGMFFV
                     NNRWLGGTLTNFRTIKQGLDRLRTLERMSEDGSYEQFPKKEVVKLEKERARLEKYLGG
                     LKNMGNLPHVMFVVDPSMEKIAVHEARKLNIPVVGITDTNCDPDQVDMVIPGNDDAIR
                     SIKLITSRVADACLEGSERRKDELAGRGDDASRDNAHSSRSRGPEPSIYRGRPRGAGG
                     GGAHRN"
     gene            4034..5083
                     /locus_tag="PK710_16505"
     CDS             4034..5083
                     /locus_tag="PK710_16505"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HOT11385.1"
                     /translation="MNDAVLFGQKGWFVKVVHLNYILLGILAGLIYRNVLFGGKIPAV
                     LEDGFKLSRLFIKTGIILLGSLYTVQAIVQLGIMAVLLIGGFVILTITLVLWLGRVFG
                     LDRSMTGVLANALSICGVSAAVATSPAVEAKASHVAYSIATILGFGILTMFISPFIGH
                     ALDLTDLQYGAWIGTGILNSGQVLAAALAFNPQVAPGTAVSIAEIWNIMRVISIPFAV
                     FAVTLWYWSGKSAEKGDSTTAQRKGLGSLLVEKFPVFVIGFIAMVVLTSLGAFGDVGI
                     KGGPPASETIKMLRLWMTWIFGFGLVGLGAYIDFKELAQAGGAPLKVGLIVGITKYVL
                     ALLVVLLIRDYLVAI"
     gene            5099..5341
                     /locus_tag="PK710_16510"
     CDS             5099..5341
                     /locus_tag="PK710_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11386.1"
                     /translation="MADSQQDDKNQGSPSENKMTLFRDDRSEDIGAILLAAVVIVTIV
                     VLTMGKNPSSSNAPSPSSSAATSAGSVENPPASSRP"
     gene            5382..5807
                     /locus_tag="PK710_16515"
     CDS             5382..5807
                     /locus_tag="PK710_16515"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT11387.1"
                     /translation="MDGSDASQTALEQAVWLARHANAVLTGFYILDTGWADFIGNDWQ
                     SSDGARRGFLDHIGKEQTSQAELARLQFETAVQGLAQAQFIVLTGDPIRVLVERLNDP
                     RSDLLVFGRKTFQVSGRPSLKTAAQSLVKQGRKPVLLLP"
     gene            complement(5858..>6169)
                     /locus_tag="PK710_16520"
     CDS             complement(5858..>6169)
                     /locus_tag="PK710_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11388.1"
                     /translation="GGGGGASGNEGGGTVDDSGVSGGSGGMMSDSGVSDGSGAGASIS
                     KDSSKDSKDGCACGVVNRHHGSGLWLMIPLMTLLGRRRWCRSEATADRGLRRSHRLAF
                     L"
BASE COUNT         1285 a         1897 c         1646 g         1341 t
ORIGIN      
        1 tcttgccgct tctcacccac cagcgaaatt cccctggtcc cactggaacg ccctgcgggt
       61 cgaccgccag gcaggtgatg accttgatgc cagtgccctg cttatgtcct gagcccagtc
      121 gtcccatgcc tttggtcttg cgagagtcag gcagactcga gctggagccg tcgacagcca
      181 gccaaacgaa cggctcgcgg gcgatttgcc gtgccgcggc tactgcggcg gcgtggccga
      241 ccgcctcggc ggagatacgc tcattttcga cgaaatcgta ggctgcttcg cgttcgcgcg
      301 ggtcagtcat cccttggctg atggttcctc cgccgccgcg caccacggtg gcagcgattt
      361 tcaggagcct cttatcgttg cgcaggtctc ccaacttcgc ggttccgaac tgtaaaaatg
      421 cccattccct ggcttgtatg tcgtccatgc cgtgtccaac acacgaacag ggatggccat
      481 tccctaactt tgtgaaattg ttcattaagt tgagtatgga gccagatcac agaaataaac
      541 ggggggcagg gcatgggggc aactagcaac agctcgctgg cgacgaggcc acggctgctt
      601 caaccccggg cgccgaatgc ttccgccgct tcgtccagac gaacaaatgc ataccccgat
      661 gcccgaagcc tctgaatgac ccccgcgaat atcaatagct tgcgatccaa cgatatgcgg
      721 atgtccggtt gatgcccctc cagagccgac aaaccatcga accgatcaag ccaatcgatt
      781 ccatgcagct cgagattgac cagcggctcg ccaatcacca tgcgcgtcag ccagagagca
      841 cctttttctc ccgcaagcgt caaactcgtt ccaatatagg gcaagcgcaa tccccgggtg
      901 acttgaatcg gcaattcgac cggcccctgc ccccgacacc gaaacggacg cccaagacga
      961 taagggcgcg tgggcgcgcg taacgttctt ggatgatcca aaatggaccg gctctccctt
     1021 ccttggaaac gaatccgacc catcgccagc gccttggccg cgtggtaagc cggacaagga
     1081 aacacggaag agtcgtactt caccccggct tcccgcaaca catccacgag ttcatccgtg
     1141 atcagataac caggcgcgcg aaagccaccc ggacgaacgc cggtatgccg ttcgatcgcc
     1201 tccgccgcat cgatgacctg cgccttcatc tcctctttgc tccgacgcgt cagatcataa
     1261 aagtgatcga aggagtggtt ggccagctcg tgcccacggc tcgcaagtct ttgtaatatt
     1321 tgactatttt ttgaatgcgc cagatccgaa gcgattacga ataaggtgag agggatgttt
     1381 tgagaagccg cgaaggcctc caaccgctcc agagcaacct ggtagacaac atgagccagg
     1441 tcccccgaag gcggtggaag gccgtggata tcgaagtaat tcgttatctc atcgaggtcg
     1501 acggagaccg cacagaggcg aggtgcggga agaggcatca tcgatacgga tcaggagcca
     1561 gccattttct tggcttcttc ggcgaagtct tcctcggcgc gggacagtcc ctcacccaat
     1621 tggaaacgaa cgaatccgag tagggtgatg gatccaccag cttgttgggc agcttccgca
     1681 cgaacctggt cgacactctt tccgttttcg atgacggaat cctggtcgac gacgcatagt
     1741 tcggtgtacc acttattgac cttgccctcg atgattttgg gccaggcaac ctcgggcttt
     1801 ccttcttgtt tgagttgttc ggcgaaaatc tctttctgct tctcgatgtc cgccgcgggc
     1861 acctggttgc gatcgagata caaggggctc atggcggcaa tttgcatggc ggtatcgtcg
     1921 acgaacttca gataggcctc gtgtttggcg accgcgtcgt tttcggcggc ggtggcaagc
     1981 agaacaccaa tttttccgtt catgtgaacg taagaatgca cgttgccggc gggagcatcg
     2041 agggtaagcc gctgcatcct tcgcacgtcg attttttcgc caatcgtcgc gatgagatcg
     2101 tcgcgaattt cggccacggt ttttccggcg gccatcttgg aagcaaggag ttcgtcgagg
     2161 ttgttgacgt ttccaacgag ctgagccacc tcgtcgacga aggctttgaa cttgtcgttt
     2221 cgtgcggcaa agtccgtctg gatattgatt tcgacgagcg ttccggcgcg tccgtcggtg
     2281 gccatgttgg cgcggatttc gccctcggtg gcggtagctg tcgcacgctt ggcgctcttg
     2341 gcctttcctt ttttgagaat gatttcaaca gccttgtcga tgtcgccttc ggcttctacc
     2401 aacgcgtttt tgcaatcggt cataccggct gccgtgcgct ctcgaagctc acggatgagc
     2461 tggggggtaa cgctagccat gagtcataca ctccttatca ctcggtccgc ccgctggatg
     2521 ttggacggac cgaaccgaat caaagatggg aaagggaaaa tcagttgcga tgcgcgccac
     2581 cgccccccgc accacgagga cgtcctcgat aaatcgaagg ctccggaccg cgtgaacgag
     2641 aggaatgcgc attatccctc gaagcatcat ctccacgacc ggccagttcg tctttgcgac
     2701 gctccgagcc ttccagacag gcatccgcca cgcgcgaggt gatgagtttg atcgagcgaa
     2761 tggcgtcatc gttgcctgga atcaccatat ccacctggtc cggatcgcag ttggtatccg
     2821 tgattcccac cacgggaata ttgagtttgc gcgcttcgtg aaccgcgatt ttttccatcg
     2881 atggatcgac cacgaacatg acgtggggca gatttcccat gtttttcagg ccgccgaggt
     2941 atttctcgag acgagcccgc tctttctcga gcttgacgac ttcctttttc ggaaactgct
     3001 catagcttcc gtcttcggac atgcgctcga gagtgcgcag acgatcgaga ccttgcttga
     3061 tggtgcggaa gttggtgagc gtgccgccca gccagcgatt gttgacgaaa aacattcccg
     3121 cgcgcagggc ttcttcccgc acgatgtcct gggcctggcg tttggtgccg acaaaaagca
     3181 cgctgccacc acgagaaacg gcttcttgaa tgaagtcgta agcacgggtg aaaagcttgg
     3241 cggtttgatc gagatcgatg atgtgggtgc cgttgcgcgc gccataaatg tacgggcgca
     3301 ttttcgggtt ccatcgcttg gtctgatggc cgaaatgaac accggactcg agcaaagcgc
     3361 gtacggacaa ggctggcgag cccggtgcgc gcggcgcaaa gctgggcatc tcaggggcag
     3421 gttcttccgg agcttgaacg ggggtcactt cttctgtcac ttgcgctctc ctttcggttg
     3481 tttcctctgc ttcaccagcc cgccaactcc tgatcgagca ccggacgagc cgtttgcgct
     3541 gtgaagcgtg tgagattctt tcccttccat ggggaagggc gagcactgta gacgcatcgc
     3601 aaacgatgtc aagagccaga tgaagccgaa tccaccattt ttcaagcctg catcgaccag
     3661 agcgatttca aggaaaaatc cgccacttag gtggccccca ccacgaaaaa cgacttgcct
     3721 tgcccgcgaa tccagacaat tggtgacgtt gggcacggtt gacaacgttc gggctttgca
     3781 taggctaggc ctccgatagt caggtggctg accggttcaa ccgcgcactg ttttcgactc
     3841 tgtgccccca tttttctaga atacctgctt gaacaccgga cggcaccacc ctggcaagtt
     3901 gaaaaaccgg aaggcataga aggatattca ggtgatcgac gtggattttt tcaaaaaact
     3961 gccgcgcgcc ctgccaggcc tagccctcat cgtcgccacc atggtggtga tccgatattg
     4021 ggtggaaccc tggatgaatg atgccgtctt attcgggcaa aaagggtggt tcgtcaaagt
     4081 cgtccacctc aattatatcc tgctcggtat cctggccggc ctgatttatc gaaacgtgct
     4141 attcggcggc aagatcccag cggtcttgga agacggattc aaattatctc gccttttcat
     4201 caaaaccggc atcatcctgc tcggctcctt gtacacggtc caagctatcg tacagcttgg
     4261 aatcatggcg gttttgctca tcggcggctt cgtgatcctc accatcaccc tggtgctgtg
     4321 gctcggtcgg gttttcggat tggaccgctc catgaccgga gtgttggcca acgcgctcag
     4381 catctgcggg gtaagcgccg ccgtggctac ctcccccgcc gtggaagcca aagcatccca
     4441 tgtcgcctac tccatcgcca ccatcctcgg attcggcatc ctcaccatgt tcatcagccc
     4501 cttcatcggc cacgccctag acctgacgga cctgcaatac ggcgcttgga tcggtaccgg
     4561 tatcctcaat tccggacagg tcctcgccgc cgccctcgcc ttcaacccac aagtcgcgcc
     4621 gggaaccgcc gtatccatcg ccgaaatctg gaacatcatg cgcgtcatct ccatcccctt
     4681 cgccgtattc gccgtcaccc tctggtactg gtccggtaaa tcggccgaaa aaggcgactc
     4741 caccaccgcc caacgcaagg gcctcggctc gctgctcgtg gaaaaattcc ccgttttcgt
     4801 catcggcttc atcgccatgg tggtcctgac cagcctgggc gccttcggcg acgtcggcat
     4861 caaaggcggc ccccccgcct cggaaaccat caaaatgctt cgcctctgga tgacttggat
     4921 tttcggcttc ggcctggtcg gcctcggcgc ctatatcgac ttcaaagaac tcgcccaggc
     4981 cggtggtgcc ccgctcaaag tcggcctgat cgtcggcatc accaaatacg tcctcgccct
     5041 tctggtcgtc cttttgatcc gcgactacct cgtcgccatc tgagcaggag atagcatcat
     5101 ggcggattca caacaagacg ataagaacca aggctccccg agcgaaaaca aaatgaccct
     5161 gttccgggat gaccgttccg aggatatcgg tgccatcctg ctcgctgccg tcgtcatcgt
     5221 gaccatcgta gtcctgacca tggggaaaaa tccctcgtcc tcaaacgcac catcgccctc
     5281 gtcgagcgcg gccacaagcg ccggcagcgt cgaaaaccca cccgcctcct cacgcccctg
     5341 aacaaaaaaa acttatgttt ttccaataca tacaggttgc cgtggatggc agtgacgcgt
     5401 cgcaaacagc cttggaacag gccgtctggc tcgcgcgtca tgccaacgcg gtcctgaccg
     5461 gtttctatat cctggatacg ggatgggccg acttcatcgg caatgattgg caatcctccg
     5521 acggagcccg acgcggcttt ctcgaccata tcggaaaaga acagaccagc caggccgaat
     5581 tggcccgcct ccagttcgaa acggcggtcc aaggtctcgc gcaagcccag tttatcgtgc
     5641 tgacaggaga tcctatccga gttctggtcg aacggctcaa cgatccacgg tcggatctgc
     5701 tggttttcgg acgcaaaacc tttcaagtca gcggaagacc aagcctgaaa accgccgccc
     5761 agagcctcgt caagcaaggg cgaaaacccg tgcttctgct gccttgagga ccgaaaagcc
     5821 cgaaatatat gtaactacat gaatttagta atgtaattca aagaaaggcc aatcggtggg
     5881 accgtcgaag acctcggtcg gcagttgcct ctgatcgaca ccaacgccga cgtccgagca
     5941 gcgtcatcaa cgggatcatc aaccacagtc ccgatccgtg atggcggttc acgacaccac
     6001 acgcgcatcc atcttttgag tctttggacg aatccttgga aatagacgcc ccagcaccac
     6061 ttccgtcgga cacccctgaa tcgctcatca tgcctccgct ccccccagat acccctgaat
     6121 catccacggt tccaccaccc tcatttccgc tcgctccccc ccccccccc
//
LOCUS       DAOSOR010000339         6157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_12853_length_6157_cov_4.348738, whole genome shotgun sequence.
ACCESSION   DAOSOR010000339 DAOSOR010000000
VERSION     DAOSOR010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6157
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12853_length_6157_cov_4.348738"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PK710_16525"
     CDS             complement(<1..198)
                     /locus_tag="PK710_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOT11389.1"
                     /translation="MALDWRGQLRKAARTLSDVEQTVRLTEQERRGLHWAEHGGMPVL
                     IPPYVLSLIDTDDPQCPIRRQF"
     gene            complement(198..1250)
                     /locus_tag="PK710_16530"
     CDS             complement(198..1250)
                     /locus_tag="PK710_16530"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOT11390.1"
                     /translation="MLVSRSIHRLRWAAPLLGWFAIISTGCRDDALRRPSEDTAPPPA
                     GLSPKQAAMVLAQVGDHSITLGEYAATLEQMDQFDRLRFKTPERRRELLDEMITVELL
                     AKEAERRGLDKDPRTQEALRQVLRDALLRDARKSARAPGEFGEDEVKAYYEAHRDQYA
                     EPERRRVSHLVVKDKKKAEELLVKAKAMTDQEKWGQLVLDHSDEYKGKEYRGPVETAG
                     DLGLVGPTGDRRGANPRVDDSLRAAVFEVAEVGQMVDRVVPDASGSFHLVRLVGLTKA
                     HTRSFAEAERTIRITLAQQEIAVREQQLEEELRKKYPVTIDEAALADVKMPTTPSAAP
                     PPSDDQNDDDDHGHAH"
     gene            1455..3575
                     /locus_tag="PK710_16535"
     CDS             1455..3575
                     /locus_tag="PK710_16535"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HOT11391.1"
                     /translation="MWHTIPALGTGLLTASLVASGFTFFLAALASSRPRLLAAARRAA
                     YGTAAWTTAAVAILAYGFVTHDFRLSYVSRYSDRSMSVGYLISALWGGQDGSLLWWMF
                     LLAAYSAVCVAWLRNRYLALQPYVIAALMGTVAFFGLVTLVAADPFTLNLAGPPVDGQ
                     GLNPLLQNINMVIHPPALYLGFVGCTIPYAFAMAALVSGRLDNEWLIAIRKWMLVAWL
                     FLTLGNVLGMIWAYVELGWGGYWGWDPVENASLFPWITATAYIHSAMVQERRPMFKLW
                     NISLVSITFFLTIFGTFLTRSGVIASVHAFAESDLGIYFLYFLGLLISTTIGLIVWRW
                     SPLRSNTSIESVVSREAVFFSLNWVLIGTTTFVLVATTLPVLSNALVGRTITVGPEFY
                     NRWMTPIGLVILAIMGATPLFGWRKTNAHAVRRGFIFPATIAVLGLLLHLVFGPSLGL
                     PPFVSVTTEGDLEPLPGVLQYLGKIVPVVTVTLATFNVAVIVQEFWRGAKAQRSVRKT
                     HLLSALFHAVSRARRRYGGYIAHLGLVLVFVGFLGQTWNASGQASLVPGETFSIENYV
                     LRYERSRVERDRAKDMYIADLSVTDRTGAYRGTVSPAKYVYRSHRDQPTSEVDVLPSV
                     RDDLYVVLGSLSLESHRAAIQVYVNPLVSFIWIGVIIVVLGTGICLWPHASMSRQRKT
                     QRPDDGNGERKAALDVERKDAHAS"
     gene            3544..4143
                     /locus_tag="PK710_16540"
     CDS             3544..4143
                     /locus_tag="PK710_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT11392.1"
                     /translation="MSNERTLMQASGSSTSGWPKLWLVMIGIVPALGAVMVYGLPTGI
                     IVVGAIFLLLGIWNLWVSLQIVAGDRSVTLREDSMAFSDEKRFLLRTLEDLEYERTTG
                     KIDDDDYEELREQIRNRAQRVLSEDETELRNRAEQLVEDYLRTHEKVSTAGSKSASST
                     TTKTRQCPSCTTENDLDASFCKRCGKKLPSALSNKARES"
     gene            4140..5393
                     /locus_tag="PK710_16545"
     CDS             4140..5393
                     /locus_tag="PK710_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11393.1"
                     /translation="MRRPWLTLAVLFLSTHAMGQSPLPADDALPPGHPPIDGMMAPSV
                     DPTRSPSDDATLPSGTVVVQANGPADVVAGLQTFLIQWHQDAKGAEKQVRATASLDTS
                     GSTTFTGLSTESSYGVVIEHEGVSYASPAFRVGPRGGKRVPIRVYPITTNIDEALVGV
                     RVVMYLEPRDQHIHAEQLLQFVNLSQWTWRTPTVPIRLPPEAVAVIDDTQSGPVHIEV
                     VPEHGVVMQGVVPPGTTDVVFRARIPYPNKDRLDLSLGLPPRVGAVRIMAGLGPGLGL
                     QVAGFPQPSTSLDEKGRRLLSTELAVPAGADQIERVSVSITGFPGRNLISTIGIGLTL
                     ALIVAGLVITTADRRRSKNNRLSDRESLLEQFLLLETDLKKGKLRRSQYETNRQRLLE
                     LLKERLRDERNSSDVAVVESVGDKP"
     gene            5641..>6157
                     /locus_tag="PK710_16550"
     CDS             5641..>6157
                     /locus_tag="PK710_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOT11394.1"
                     /translation="MNITVPKKDLLRLVARCQGVVDKKSTMPVLANVLISVDSPKAIR
                     IAATDLYLSISGHTEAEVDQGGSAAVPARDLFERIKAMPDGPISISVGAGGTEVQSKT
                     SARRYRLHGLSGDSFPSLPEPAPDAPTLELEVDVLSRLIGQTQFSISTDETRAHLNSA
                     LFEWEGDRIRMV"
BASE COUNT         1261 a         1710 c         1700 g         1486 t
ORIGIN      
        1 gaattgccgt cggatcggac attggggatc atccgtgtcg atgagagaca gcacataagg
       61 tggaatcaga accggcattc caccgtgctc tgcccagtgc aatccgcgcc gctcttgctc
      121 ggtcaaacga acggtctgct cgacatccga gagcgttcga gccgctttac gaagttgtcc
      181 gcgccaatcc aaggccatca atgggcatgc ccgtgatcgt catcgtcgtt ttgatcatcg
      241 gaaggtggcg gcgccgcgct cggggttgta ggcatcttga cgtcggcgag agccgcttcg
      301 tcaatcgtta cggggtactt ctttcggagt tcttcctcga gctgctgctc acgaacggcg
      361 atctcctgct gcgccaaggt gatacgaatg gttcgttccg cttccgcaaa ggagcgtgta
      421 tgggctttgg tgagcccaac gagtcgcacc aggtgaaagg aaccactcgc atcgggcacg
      481 acccgatcga ccatttgccc gacttcggcg acttcgaaca cggcggcgcg taaggagtca
      541 tctacccggg gattggcacc tcgacgatcc cccgtgggac caacgagccc gagatcgcct
      601 gccgtttcga ccggcccacg atattctttc cctttgtatt cgtcggaatg gtccaacacc
      661 agttggcccc atttttcttg atcggtcatc gccttggctt tgaccaataa ttcctcggcc
      721 tttttcttgt ctttgaccac cagatgactt acgcgacgcc gttccggttc agcgtattgg
      781 tccctgtgag cctcataata ggccttgacc tcgtcttccc cgaactcccc gggagcacga
      841 gcgctcttcc gcgcgtcacg cagaagagcg tcacgcaaca cctgccgaag cgcctcttgc
      901 gttcgtgggt ctttgtccaa tccccgtcgt tctgcctctt tggcgagcaa ttccacggtg
      961 atcatttcat cgagcaattc acgacggcgt tccggtgttt tgaagcgcaa tcggtcgaat
     1021 tgatccattt gctcgagggt ggcggcgtat tctccgaggg ttatcgaatg atcgccaacc
     1081 tgggctagga ccatggcagc ttgtttgggg gataagccgg cgggaggcgg cgcggtatcc
     1141 tccgaggggc gtcgcaatgc atcatctcgg catcccgtgg aaataatcgc aaaccatccg
     1201 agaaggggcg cagcccacct aagtctgtgg attgaacgag acacaagcat gttgcacgca
     1261 cctagcacgc gcggctggta aaaggcaatg caggcgaaac ggacggctcg tggagccatg
     1321 agaaccgacg ttgtgccatg ggtggccgga gcgtgtctga ttgcactcga gcacgcctcg
     1381 ctttaggttg cgcctccagg tgaatggtcg gaaggacaca agctccttcg gtctttttcg
     1441 ccgaaagaca cgtcatgtgg cataccatcc ctgctctcgg aactggcctt ctcacggctt
     1501 cgctcgtggc ctccggattc acgtttttcc tggctgccct cgccagttcc cgtccacgat
     1561 tgctcgcagc cgcgcgtcgg gccgcttatg gcaccgcggc ctggaccacc gccgccgtgg
     1621 ctatcctcgc ttatgggttc gtaacccatg actttcgcct cagctacgtc tcccgatact
     1681 ccgatcggtc catgtccgtg gggtatctca tcagcgcgct atggggcgga caagatggct
     1741 cgcttttgtg gtggatgttc ttgttggcag cctactccgc ggtctgcgtg gcttggcttc
     1801 gaaatcgata cctcgccctt cagccctatg tcatcgccgc gctcatggga accgtggcct
     1861 tcttcggcct cgtaaccctt gtcgccgcgg acccattcac cctcaatctc gctggacctc
     1921 ccgtcgatgg gcaagggctc aatcccttac tccaaaacat caatatggtc atccacccgc
     1981 ccgcgcttta cctgggcttc gtgggatgca ccattcccta tgcctttgcc atggctgccc
     2041 tcgtatccgg acgcctggac aacgaatggc tcatcgctat ccgaaaatgg atgcttgtag
     2101 cttggctatt tttgacgcta ggcaacgttc ttgggatgat atgggcttat gtcgagcttg
     2161 gttggggagg atattgggga tgggatcccg ttgaaaatgc atcgcttttc ccatggatca
     2221 ctgccacggc ctatatccat tccgcgatgg tgcaagaaag aagaccgatg ttcaaattgt
     2281 ggaacatcag tctcgtttcc atcacgttct ttctaaccat tttcggtaca tttctaacga
     2341 gatccggtgt cattgccagc gttcatgcct tcgcggagtc ggatctcgga atctacttct
     2401 tgtacttcct gggccttctc atctcgacaa ccatcggtct catcgtgtgg cggtggtctc
     2461 ccttgcgatc caatacctct attgaatccg tagtttcgcg tgaagcagtg tttttttccc
     2521 tgaactgggt tcttatcggt accacgacct ttgttctcgt cgccacgacc ctgcccgttc
     2581 tctccaacgc tctggtcgga agaacaatta ccgtcggccc cgagttttat aatcgctgga
     2641 tgacgcccat tgggctcgtg atcctggcga tcatgggtgc tacccccttg tttggttggc
     2701 gaaaaaccaa tgcccacgcg gttcgacgag gatttatttt tccagcgacg atcgcggtcc
     2761 ttgggttgtt gctgcacctt gttttcggac cttcgttggg actgcctccc ttcgtttccg
     2821 tgaccaccga gggcgatctc gagcctttgc ctggtgtgct gcaatatttg ggaaaaatcg
     2881 tgccggtagt gacggtcacc ctcgccacgt tcaacgttgc cgtgattgtc caagagtttt
     2941 ggcgaggtgc gaaggctcag cgttccgtaa ggaaaaccca tctattgtcc gcgcttttcc
     3001 atgcggtgag ccgggcgcga cgacggtatg gtggttatat cgcccacctt gggcttgtct
     3061 tggtcttcgt cggttttctc ggccagacgt ggaatgcctc cggacaagct tctcttgttc
     3121 cgggcgagac gttttcgatc gaaaattatg tattgcgtta cgaacgctct cgggtggagc
     3181 gcgatagggc aaaagatatg tatatcgcgg acttatccgt gaccgaccgt acgggtgcct
     3241 accgtgggac ggtatcccct gccaaatacg tgtatcgatc ccatagagac cagccaacga
     3301 gtgaagtcga tgtgcttcca agtgtacgcg atgatttata tgtcgtgctg ggaagtcttt
     3361 cccttgagtc ccatcgggcg gcgatacagg tctacgtcaa tccactcgtg tctttcatct
     3421 ggattggcgt gatcatcgtg gtgctgggta cggggatatg tctttggcca cacgcttcga
     3481 tgagtcggca aagaaagacc caacgcccgg acgatggaaa cggggaaagg aaagcggccc
     3541 tcgatgtcga acgaaaggac gctcatgcaa gctagtggct cgtcgacatc gggatggccg
     3601 aagctatggc tcgtgatgat tgggatcgtt cctgctttgg gtgccgtaat ggtctatggt
     3661 ttgcccacgg gaatcatcgt tgtcggtgcc atttttttgc tgcttggcat ttggaacttg
     3721 tgggtgagcc tgcaaatcgt ggctggggac cgatccgtga ccctacgaga agattcgatg
     3781 gctttttccg acgagaaacg atttctgctt cgaaccctcg aagaccttga atatgaaaga
     3841 acgacgggaa agatcgatga cgacgactac gaggaattgc gagaacagat tcggaatcgt
     3901 gcccagcgag tgctaagcga agacgagacc gagcttcgga accgcgccga acaactcgtg
     3961 gaagactatc ttcgaaccca tgagaaggtc tccaccgctg gttcgaaatc cgcatccagc
     4021 acaaccacga aaacacgcca atgcccttca tgcaccacgg aaaatgactt ggatgcgtcc
     4081 ttttgtaagc ggtgtggcaa gaaactccct tccgcgctca gcaacaaggc acgggaatca
     4141 tgagacgacc ttggctcacg ctagccgtcc tatttttgtc cacgcacgca atgggtcaaa
     4201 gcccattgcc cgcggatgat gcgcttccac cggggcatcc acccatcgat gggatgatgg
     4261 ccccttcggt cgatccaacc cgctccccat cggatgacgc tacgcttcct tccggaaccg
     4321 tcgtggtgca agcgaatggc cccgcggatg tcgttgcggg acttcaaacc tttttgattc
     4381 agtggcatca agacgcaaaa ggtgcggaaa agcaggtgag agcaaccgct tccctcgaca
     4441 cgtcgggatc gacgacattc actggtttat ccacggaatc ttcctacggt gtcgtcatcg
     4501 agcatgaagg agtgtcctat gcttcgccgg cctttcgtgt ggggccgagg ggaggaaaac
     4561 gggttcccat ccgtgtgtat cccatcacca cgaacatcga cgaagcactc gttggcgtgc
     4621 gtgtcgtgat gtacttggag ccgcgggacc aacatattca cgcggagcaa ttgcttcaat
     4681 tcgtcaattt gagccagtgg acttggcgta cgccaaccgt tccgattcgg ctgccacccg
     4741 aagcggtggc ggtcatcgac gatacgcaat caggtcctgt ccacatcgaa gtcgttccgg
     4801 aacatggtgt ggtgatgcag ggggtcgtac ctccgggaac caccgacgtt gtttttcggg
     4861 cacggattcc ttatccgaac aaggatcgat tggacctcag cctcggattg ccgccacgtg
     4921 tcggagctgt tcgtatcatg gccggattgg gaccagggct cggcttgcag gttgccggct
     4981 ttcctcaacc atccacctcg ctcgatgaaa aaggtcgtcg ccttctatcc accgagcttg
     5041 ccgtaccagc cggagccgat caaatcgaac gggtatccgt aagcatcacg ggttttcccg
     5101 gcagaaatct catttccacg ataggcattg gattgacgtt ggccttgatc gtagcaggcc
     5161 tggtcatcac caccgcggac cgtcgtcgct ccaaaaacaa ccgattgtcg gatcgtgaat
     5221 cattgctcga gcaatttctc ctcctcgaga cggacctaaa aaaaggaaag cttcggaggt
     5281 cacaatacga gacaaatcgc caacgattac tcgaattact taaggaaagg ttacgagacg
     5341 agcggaattc atcggatgtg gctgttgtgg agagcgttgg ggataagccg tgatcttctg
     5401 gtggatcttc gtgggagtta tccactttta cgcaatgcgt catgaggatt tccacaattc
     5461 tccccatgga aatccttgga gcatccacag ccgtcaatgt tataactcat tgaattttat
     5521 atatatttag aactatcccc atcattcaca ccccctatcg acaccatctc agtttaatga
     5581 gaaggagacc agaagagaga atcgcttctt gggcaagcca agaagaccag cacaacgccc
     5641 atgaatatca ccgttccgaa aaaagacctg cttcgtctcg tggctcgatg ccaaggcgtg
     5701 gtggacaaga agagtacgat gcctgttctt gccaacgtgc tcatcagtgt cgattcgccg
     5761 aaggccatac gcatagcggc gacggatttg tatttatcga tcagcgggca tacggaggct
     5821 gaggtagacc aagggggtag cgcggcagta ccggcgcgag atttgttcga gcggatcaaa
     5881 gcgatgcctg acggtccgat ctcgataagc gtgggtgccg gaggaaccga ggttcaatcg
     5941 aaaacatcgg cgcggcgata tcggcttcat ggtttgtcgg gggattcatt tccttcgtta
     6001 cctgaaccgg cgcctgatgc gccaacgctt gaactggaag tggatgtgct atcgcgtttg
     6061 attgggcaaa cgcagttttc catttcgaca gatgaaacgc gcgctcattt gaacagcgcg
     6121 ctattcgagt gggaggggga taggattcgc atggtga
//
LOCUS       DAOSOR010000340         6144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_12883_length_6144_cov_4.596650, whole genome shotgun sequence.
ACCESSION   DAOSOR010000340 DAOSOR010000000
VERSION     DAOSOR010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12883_length_6144_cov_4.596650"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /gene="nrfD"
                     /locus_tag="PK710_16555"
     CDS             <1..619
                     /gene="nrfD"
                     /locus_tag="PK710_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HOT11395.1"
                     /translation="GLGGRPLWNSALLAPRFLASAFVAGPAFLIVALHALARFTSFHV
                     EERPVRILSSILRITVLLNLFMLVSEVFTEFYSGGHHTASARYLFFGLHGHHVLVPWI
                     WTAIGLNVAAAIALFHPRARTSVPLLIAACVATFIGVWIEKGMGLIVPGFIPSPLHEI
                     VEYQPSVVEWKVTAGIWAFGLLVYTAALKIAVPVITGKPVSAKKH"
     gene            629..1033
                     /locus_tag="PK710_16560"
     CDS             629..1033
                     /locus_tag="PK710_16560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOT11396.1"
                     /translation="MLSKSAEAAIAAMGRLAQAQCRGEPPLTAVQVAEDRGLQRPYAA
                     KLLTVLSQHGLVIGTPGPNGGYRLARAAAEISLLQIVVCFERPNLSSPCMFHPEHCGG
                     DYPPCPLHHGLERLRSERDTFLRETTLADFCV"
     gene            complement(1042..2610)
                     /locus_tag="PK710_16565"
     CDS             complement(1042..2610)
                     /locus_tag="PK710_16565"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT11397.1"
                     /translation="MTANEQTECINPATGEIIGYSALHSIDDLRAMIERSRQAQRQWA
                     STPVAQRARAVLQIRDGIAQNADDIAAIISRDNGKTRTDALATEVLPAIMAADYYANN
                     AKRFLEDRILKPGNPLLANKISKIIRVPFGVIGIISPWNYPFAIPFSEVIMGLLAGNG
                     VLLKVATETQMTGRALERAIKAAQLPPDLFAYVNVPGRLAGDALLENGIDKLFFTGSV
                     PVGKKLMAKAAETLTPVSLELGGNDAMLVCPDADLDRAAAGATWAGFQNAGQSCGGVE
                     RIYVHHEVYDVFLDKLAQRVRALRVGPDIDFEVDIGAMTTVSQLRTVQAHVADALEKG
                     AIIFAQSAAVQDDSRFHPAMVLTNVNHDMLVMKEETFGPVVGVMKVQSMDEAIALAND
                     STLGLTGSVWSNNRKQAERIGRRIRAGAITINDHLMSHGLAETPWGGFKQSGIGRTHG
                     EIGFAEMTEPQCIVQDYLPAVRKNMWWHPHGKSVYDGLRGIIDLLYAKETGRKASGVG
                     QLAKLFPRTFTNEG"
     gene            complement(2725..3537)
                     /locus_tag="PK710_16570"
     CDS             complement(2725..3537)
                     /locus_tag="PK710_16570"
                     /inference="COORDINATES: protein motif:HMM:NF012571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11398.1"
                     /translation="MDTITIELSADHPGFNDPAYRQRRNLIANLAGAHIPGTPAPTVS
                     YTDVERETWGTVFEQLMRLYPSHACSAFVQIIGEMGYHAEQIPQLRDVSAFLEQRTGF
                     RLEPVAGLVSPRDFLSALGRGVFCATQYIRHHSMPHYTPEPDVIHELMGHAPMLAIED
                     FANLSRKIGEGAARASDEQVKQLATLYWFTIEYGVLRENGNLKAYGAGLLSSFGELQH
                     AIDGHPTILAFDPEVAKDHSYPITTYQPLLWEVPSIRKAFEFMNDFVSRHIP"
     gene            3644..5536
                     /locus_tag="PK710_16575"
     CDS             3644..5536
                     /locus_tag="PK710_16575"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HOT11399.1"
                     /translation="MGRKALVFGVMASVVVGVVVGCSATSSDNYGNGPGSGGGSGGDG
                     HDGGIEGGMFGCGTCVGSRYQPCDEHGNPLPEQNCEPDYCAPQLGCRPCMPGANTCVG
                     NVVHECTADGQIGAMVQECDISQGQMCGNGKCGTACDIAADQASNVGCEFWAVDLDQQ
                     DYCKGLTCNDPASAPWGIVLSNASQYAANVTVELNSAPYGQEPQPVVVHQVTVKPGDL
                     QALVLPTRELDCGVKPNDYKSPGTCLSSQAFRITSSNPILVYQFNVFENAFSNDASLL
                     LPTSALGRIYRIIGWNAGHPIGMPNLGFEVDRSYVTVVGTTPSTQVTVRPSWRIRGNP
                     PIAATLPGGEIIVTLGPFDVLNLETDDGKLQDDQKTMADLTGTIVVANQPVAVFSGVE
                     STSVPHWVDIPAPPGGKPETSCCLDHLEEQMFPVESVGSKYVIPRSPVRSTGSWREPD
                     VLRIMGVAETAVVKTTLPAPYDQFTLEPGQVVTTWTQNDVVVDATKPVMVGQLLVSQA
                     WVDGPAIGDPSLTVFPPVDQFRTEYVILTPSSWDLNWVVIVAETGTEVTLDGQPTSTC
                     VSEPSTLLDGKAYQSRRCPLAVGVHRLSGNGPFGIAAYGYGRAGSYAFVGGADVKRIY
                     DPPPLK"
     gene            5729..>6144
                     /locus_tag="PK710_16580"
     CDS             5729..>6144
                     /locus_tag="PK710_16580"
                     /inference="COORDINATES: protein motif:HMM:NF013358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR1/FUN34/YaaH family transporter"
                     /protein_id="HOT11400.1"
                     /translation="MIRRVDIGLGPDKGRLGDPTPLGLIGLAIACGALTPIAFGLSVT
                     PSALMTAAVFAILFGAGCQLLSGLMNFVNKNAFGGTIFTAFSFLWMVNGWSLYSLAKG
                     EVPDHTVGLATEIVMIVIFVVLTYGFGFFSRLLFYFL"
BASE COUNT         1185 a         1507 c         1971 g         1481 t
ORIGIN      
        1 ggggcttggt ggaaggccac tgtggaattc ggcgcttctt gcccctcgtt ttctcgcctc
       61 cgcgttcgtg gcgggtcccg cgtttttgat cgtcgccctg cacgcgctgg ctcgttttac
      121 ctccttccat gtggaagagc gtcccgtgcg gatcttgtcg agcattttgc ggattacggt
      181 gcttttgaac ctatttatgc tggtgagcga ggtgttcacg gagttttatt ccggggggca
      241 ccatacggca tcggcccgct atttgttctt cggactacac ggtcatcatg tgctggttcc
      301 ttggatctgg accgccattg ggctgaacgt ggcagcggcg atcgcgctat ttcatccacg
      361 ggcgcgaacc agcgtgcctc tgttgattgc ggcatgcgtg gcaacattca tcggcgtctg
      421 gatcgaaaag gggatgggac tcatcgtccc agggttcatc ccaagcccgc tacacgaaat
      481 cgtggagtac cagccaagtg tcgtggagtg gaaagtgacc gctgggatct gggctttcgg
      541 actgctcgtc tacacagcgg cactcaaaat cgcggttccg gtgattacgg gcaagcccgt
      601 gtctgccaaa aaacattgac gaggttgcat gctcagcaaa tccgccgaag ccgcgattgc
      661 cgcgatgggc cgtctggctc aggcgcaatg ccgaggtgag cctccgttga cggcggttca
      721 agttgctgag gatcgtggac tacaacgtcc ctacgcggcg aagctactca cggtgctctc
      781 acagcatggc ctcgtcatcg gaacgccagg tcccaatggt ggataccgat tggctcgcgc
      841 cgcggccgag atttcattgc tacagatcgt ggtgtgtttc gaacgaccga acctttcttc
      901 cccctgtatg tttcatcccg agcattgtgg aggagactat cccccttgcc ctttgcatca
      961 tggtctggag cgattgcgaa gcgagaggga cacgtttctt cgcgagacta cattggcaga
     1021 tttctgtgta taattgacag gttagccttc gttggtgaag gtgcgaggaa agagtttcgc
     1081 gagttgtccc acgccggagg ctttgcgtcc tgtctccttg gcgtaaagca gatcgatgat
     1141 gccgcgcagc ccgtcgtaga cgcttttccc atgggggtgc caccacatat tttttcgcac
     1201 ggcggggaga taatcctgga cgatgcactg gggttcggtc atttcggcga acccaatttc
     1261 tccgtgggtg cgtccaatac cggattgttt gaaccctccc cagggggttt cggcaaggcc
     1321 atgactcatc aggtgatcat tgatggtgat ggcaccagcg cggatgcgtc gtccgatacg
     1381 ttcggcttgt tttcgattgt tggaccatac cgatccggtc aatccgaggg tggagtcgtt
     1441 ggcgagcgcg atggcctcgt ccatggattg gactttcatg acgccgacca cgggaccgaa
     1501 ggtctcttct ttcatgacga gcatgtcgtg gttgacattg gtgagcacca tggccggatg
     1561 aaacctcgag tcatcctgca cggcagcgga ttgggcgaaa atgatggcgc ctttttcgag
     1621 ggcgtcagcg acgtgcgctt gcaccgtgcg aagttgtgac acggtcgtca tcgcgccgat
     1681 atcgacttcg aagtcgatat cgggtccgac acgaagggcg cgaacgcgct gcgcgagctt
     1741 gtcgaggaac acatcataga cttcgtggtg gacgtagatt ctttcgacgc ccccgcagga
     1801 ttgtcccgca ttttgaaagc cagcccaggt ggctcccgcg gcagcccgat ccaagtccgc
     1861 atcgggacaa acgagcatcg catcgttacc gcccagttcg agagatacgg gggtcaatgt
     1921 ttccgcggct ttggccatga gtttttttcc gacggggacc gagccggtga aaaacaactt
     1981 atcgatgccg ttttcgagca acgcatcgcc tgccaatcgt cctggcacgt tgacgtaggc
     2041 gaagaggtct gggggcagtt gtgcggcttt gatggctcgc tccaaagctc gtcccgtcat
     2101 ttgcgtttcg gtggcgactt tgagcagcac tccgtttccg gcgagcaagc ccatgatgac
     2161 ttcggagaaa ggaatggcga acgggtagtt ccaaggggag atgattccga tgacgccgaa
     2221 gggcacgcga atgatttttg atattttgtt ggcgagcagg gggttgccgg gtttgaggat
     2281 tcggtcttcg agaaatcgtt ttgcgttgtt ggcgtagtag tccgcggcca tgatcgcggg
     2341 aagcacttcg gtggcgagcg catcggtgcg ggttttgcca ttgtctcggc tgatgatggc
     2401 agcgatatcg tcggcgtttt gggcgatacc atcacgaatc tgaagtacgg cgcgagcccg
     2461 ttgcgccacg ggtgtcgaag cccattgtcg ttgtgcttgt cgagatcgct cgatcatcgc
     2521 ccgcagatca tcgatcgaat gcagcgcaga gtagccgatg atctctcctg tggccgggtt
     2581 gatgcattcc gtttgctcgt ttgctgtcat cgtttccccc tggggtggtc gattggattc
     2641 gagtatcaaa gagtggatgc tctttggcaa tcgcatcgaa tcatgcgggc ggcaagcggc
     2701 gcctggcatg ggcgtccggc caggtcacgg gatgtgccgg cttacaaaat cattcataaa
     2761 ttcaaatgct ttgcgaatgg aggggacttc ccagagcaga ggttgatagg tggtaatggg
     2821 gtaggaatgg tctttggcga cttcggggtc gaaagcaagg atcgtgggat gcccgtcgat
     2881 ggcatgttgg agttctccga agctggagag aagtcccgcg ccatacgctt tgaggttgcc
     2941 gttttcccga agcacgccgt attcgatcgt gaaccaatac agcgtggcga gttgtttgac
     3001 ttgttcgtcg gaagcgcggg cggcgccttc gcctattttt cgagataagt tcgcgaaatc
     3061 ctcgatggcg agcatggggg catggcccat caattcatgg atgacatcgg gttcgggagt
     3121 gtaatggggc atgctgtggt ggcggatata ttgggtggcg caaaacacgc ctcgtccaag
     3181 ggcagaaagg aagtcgcggg gactgacgag gccggctacc ggttcgaggc gaaagccggt
     3241 tcgctgttcg agaaacgcgc tgacatcgcg aagttgtggg atctgttcgg cgtggtaacc
     3301 catctcgccg atgatctgga cgaaagcaga gcacgcgtga gatgggtaaa gccgcatgag
     3361 ttgttcgaaa accgtgcccc aggtttcgcg ttccacgtcg gtataggaaa ccgtgggagc
     3421 gggggtaccg gggatatgag cacccgcgag gttggcgatg agattgcggc gttgccgata
     3481 ggcaggatca ttgaatcctg ggtgatcggc actgagttcg atggtgatcg tgtccatggc
     3541 acctccgaga gaaacaccct ggggaaggaa cgcccgaatg tccagcagga ttcacggtta
     3601 cggatgaagc gctcgatgcg caatcatggt aaggttttcg atcatgggac ggaaagcatt
     3661 ggtattcggc gtgatggcga gcgtggtcgt cggtgttgtt gttggatgct cggcgacatc
     3721 ttcggacaac tatgggaacg ggcctggatc aggaggagga agtgggggag acggccacga
     3781 tgggggcatc gaaggaggga tgtttggctg tggcacatgc gtggggagtc gatatcagcc
     3841 ctgcgacgag catggaaatc ctcttcctga acagaattgc gagccggatt attgtgcgcc
     3901 tcaactcggt tgccgtccct gtatgcccgg ggccaatacg tgcgtaggca atgtcgttca
     3961 tgaatgcacg gccgacgggc agattggagc gatggtacaa gagtgcgaca tatcgcaagg
     4021 acagatgtgt gggaacggaa agtgcggaac ggcatgtgat attgcagcgg atcaggcatc
     4081 gaacgtaggt tgtgagtttt gggcggtgga tctggatcag caggattatt gcaaggggct
     4141 aacgtgtaac gaccctgcga gtgctccatg gggaatcgtt ctttccaacg cgagtcaata
     4201 tgcggcgaac gtgacggtcg agttgaactc cgctccctac gggcaggagc cgcaaccggt
     4261 cgtggttcat caggtgactg tgaagcccgg ggatttgcag gcgctcgtcc ttcccacgcg
     4321 tgagttggac tgtggtgtca aaccgaatga ttacaaatca cccggtacgt gtttgtcgtc
     4381 tcaggcgttt cgaatcacgt cttcgaatcc gatcctcgtg tatcagttca acgtgttcga
     4441 gaacgcgttt tccaatgacg cttcgttgtt gctacccacc agtgcgctcg gccggatcta
     4501 tcgcatcatt ggttggaacg ccgggcatcc cattgggatg ccaaaccttg gatttgaagt
     4561 ggacaggtct tatgtcacgg tggtgggcac aacgccttcc acgcaggtca cggtgcggcc
     4621 gagctggcgg attcgaggaa atccgccgat tgccgcgact ctccctggtg gcgaaatcat
     4681 cgtgacgttg ggtccattcg acgtgttgaa cctcgagacg gatgacggaa agttgcagga
     4741 cgatcagaag acgatggcgg acttgacggg aacgatcgtt gtggcgaatc agcccgtggc
     4801 tgtgttttcc ggtgtcgaga gcacatccgt tccccattgg gtggatattc ccgcgccgcc
     4861 aggaggcaaa cccgaaacct cctgttgtct cgatcatctc gaggagcaga tgtttccggt
     4921 ggaatcggtg ggttcgaagt acgtgattcc gcgcagtccc gtacgttcca cgggttcatg
     4981 gcgcgagccg gacgtgttgc gtatcatggg tgtagcggaa acagcggtgg tgaaaacaac
     5041 gctccccgcg ccgtatgatc aattcaccct cgaacccggg caggtagtca cgacatggac
     5101 gcaaaatgac gtggtggttg atgcgaccaa gccggtgatg gttgggcaat tgttggtgag
     5161 tcaggcgtgg gtggatggtc cggcgattgg cgacccttcc ttgacagtat ttccgccggt
     5221 ggatcaattt cgaacggaat acgtcattct gacgccttcg tcgtgggatc tgaactgggt
     5281 agtgatcgtg gccgagaccg gaaccgaggt tacgttggat ggtcagccta cgagcacctg
     5341 cgtgtcggaa ccttccactt tgttggatgg caaggcgtat caatcccgtc gatgcccctt
     5401 ggccgtaggc gtgcatcgcc tgtccggaaa cggacctttt gggattgcgg catacggata
     5461 tggtcgtgcg ggttcgtatg cgtttgtggg cggggcggac gtcaaacgta tctacgatcc
     5521 acctcctctg aagtaacgca acgatcgcgc gattgctatg gggtcgtgtc acggttacga
     5581 gcccatcttt gggcttcggt gcatgggcga agcttgcaag cgtgcgagag atggctctca
     5641 ttcaccaaac gagggagtgt tggcgatcaa agcaattgga tgtcgattgg agtagcgtaa
     5701 tgagtgtttt gatgtgtgga ggacatctat gatcagaaga gtggatattg gcttggggcc
     5761 ggacaagggt cgattgggtg atcccacgcc gttgggactc attggattgg cgattgcgtg
     5821 cggtgcgttg acgccgattg cgtttggttt gtcggtgacg ccttccgcgc tgatgacggc
     5881 ggcggtattt gcgatcctgt ttggcgctgg ctgtcagctt ctttccgggc tcatgaactt
     5941 cgtcaacaaa aacgcgtttg gaggcacgat ttttacagcg ttttcatttt tgtggatggt
     6001 caatgggtgg agcttgtact cccttgcgaa aggcgaagtc ccagaccata cggtaggtct
     6061 tgccacggag atcgtgatga ttgtgatctt cgtggtgctt acgtatggct ttggtttttt
     6121 cagtcgtctt ttgttctatt tcct
//
LOCUS       DAOSOR010000341         6138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_12906_length_6138_cov_5.073319, whole genome shotgun sequence.
ACCESSION   DAOSOR010000341 DAOSOR010000000
VERSION     DAOSOR010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12906_length_6138_cov_5.073319"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PK710_16585"
     CDS             <1..433
                     /locus_tag="PK710_16585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HOT11401.1"
                     /translation="RRGLSTTMVGTAAAGTSRSTSTSLITSRSSTEWVSVCSAGSGGG
                     AGSGGTAGSGGSAEAGAGGEGASAGTGGKSESGGSGGSGGKAGSSNAASAASSDAEDD
                     EGCGCRVAHSPAVPWSWAIVVGMMGLAWIRKRKTQGSRTRV"
     gene            606..1061
                     /locus_tag="PK710_16590"
     CDS             606..1061
                     /locus_tag="PK710_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11402.1"
                     /translation="MRTWQWAILALALSVSGCCGKLAEKITGKAIEKAVEESSGGDAS
                     LSLDGNIDISDIPAAFRYPGMKGVARIAQNTSTSTGTVYMLESEDSLEKVKTHYEAIA
                     GYKQISKLEQPGIDPVFTYETAPKESCIIQLSTRSAKTAIILTFAKEKT"
     gene            1295..3157
                     /gene="thrS"
                     /locus_tag="PK710_16595"
     CDS             1295..3157
                     /gene="thrS"
                     /locus_tag="PK710_16595"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOT11403.1"
                     /translation="MPIIAKKIERDEREECLYRIRHSTAHLMAEAVQVLFPGTKLAFG
                     PPVQDGFYYDFDSDHRFTDEDLKTIESKMRELAKKKSPFVKKSISKAEALRLFEQQGE
                     KLKAEHVQTLSDGEITLYESGGFVDLCAGPHVESTAQLEHFKLTNVSGSYWRGDEKRE
                     RLQRIYGTAWDSKQALKAHLARIEEAKKRDHRVLGKELGLFEFPELAGPGLPFYLPKG
                     ARVLQQLKDWMWQLHITGGYGHPDKTYEPLATPHILRTDAWKISGHLENYRENMFMVY
                     SLDELDQGLLTRESREDSQTNDKDGLGNYGLKPMNCPGHVMLFNVGTKSYRDLPARYF
                     EFGTVYRYERAGTLHGMLRVRSFTQDDAHLFCTPDNVAREIEGVFDFCCHILDTFGFD
                     YHVGLKTRPESRLGTDEIWDMAENGLRAVLEKRAAGKYYVQEGDATFYGPKIDFTIRD
                     CLGREWQGGTIQLDFNLPERFELEYTDADNTRKRPVMIHRAILGSFERFFGLLIEEFG
                     GAFPLWLAPIHVRVLPIGDTQLSYAQSVVGELKRRGFRAELDDSNDKLGKKIRNGKMD
                     KVPYLVVVGQKEVDDKTITTESYHDGKLEDVTTVDALSDRMNDEIERRVFRRRA"
     gene            complement(3170..4900)
                     /locus_tag="PK710_16600"
     CDS             complement(3170..4900)
                     /locus_tag="PK710_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOT11404.1"
                     /translation="MGNEQKETITESAEGEVLGLDAAGVEALREQLAAWRNGPVAATI
                     DRIPLRKERFTTWSDVDLPDVATPLDAPIDYAHDLGMPGMFPFTRGVQPTMYRGRLWT
                     MRMFAGFGTPEQTNQRFKFLLSQGQTGLSTAFDFPTLMGYDSDSPRALGEVGMCGVAV
                     DSLRDMEVLFDGIPLDRVTTSMTINGPAIVLLAFYIALADKRGIARSKIGGTVQNDCL
                     KEFIAQHAWLVPPRPAMRMVADSIEFCASEVPRWNTVSISGYHIREAGATAAQELAFT
                     LADGLAYVRHALERGMDVDAFSPRLSFFFDVHNDFFEEIAKFRAARRMWARFMRDRFG
                     AKSRASLKLRTHAQTAGVALTAQQPYNNVVRVALQALAAVLGGTQSLHTNSLDETYAL
                     PTEEAATIALRTQQIIAEESGVTGTIDPLAGSYFVEWLTHKLEKDAMAIIEHIDSLGG
                     MVEAVEKGYPQREIAASAYRFQRQLEAGERVMVGVNKYTQHEDAKIPLLRIDESVQRA
                     QCDALARVKASRDESQVKQCLEEIRTAAQGTKNLMPSIIEAAKAYCTEQEICDVFRAV
                     FGSYSDPAEF"
     gene            4998..>6138
                     /locus_tag="PK710_16605"
     CDS             4998..>6138
                     /locus_tag="PK710_16605"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT11405.1"
                     /translation="MRPEVGQIIAGKYRLTRLIGEGGMGSVFEANHEYLGSAVALKFL
                     NPILASRPALVARFLQEARVSASIRSPHVVQVSDVDQTLEGLPYLVMELLEGESLQTC
                     MNRCRTLPLGTALEYAVQILNGLESAHGRGVVHRDLKPDNVFIVPTPHGALLKLLDFG
                     IAKLRSIADEQPGLTRPGSVMGTLEYMAPEQALSADRADLRADLYSVGVILFEMIAGV
                     RPLEADDPQTMAAQVMAGQIRRLNDIVSGIPQPLSDTIARALAGQPAQRFQSAAEMRT
                     ALLPFFTASVQAVGMPHYQSMVALPAAAPARSPTAVLADPAISASDRPAPTQPGTDPG
                     QPAGVSPTLPPSESAELVAGRTGTVLGDAAGAFPEGFGATAQMQPM"
BASE COUNT         1307 a         1633 c         1890 g         1308 t
ORIGIN      
        1 gcgcaggggc ttgtccacca ccatggtggg cacggcagcg gcgggcacct cgcgcagcac
       61 ctccacctcc ttgatcacct cgcgcagctc gaccgaatgg gtcagcgtct gcagcgcggg
      121 ttctggcggc ggcgccggtt cggggggcac ggccggatct ggcggttcgg ctgaggcggg
      181 tgcaggaggt gaaggtgctt ctgcaggcac cgggggcaaa agcgaaagcg gtggaagcgg
      241 cggatcgggc ggaaaagctg gcagctccaa cgcggccagc gctgcctctt ccgatgccga
      301 ggatgatgaa ggctgcggtt gccgtgtagc gcactctcca gccgtgccct ggtcctgggc
      361 gatcgtggtt gggatgatgg ggttggcctg gattcgaaag cgtaagaccc aagggagtcg
      421 cactcgcgtt tgacccgacg ctcgccccct tgtcattgcc tttttgccct cccagcttgc
      481 cgcccagaga aagacaatca tacggtgaaa gcaaaatagg cgcgcaagac accttgtgct
      541 accaaccacc gtgtgaccgt cggttccgct gattgaaaaa cagggaatca tccgaaggag
      601 ccatcatgag aacgtggcag tgggcaatct tggcgctggc tttgagtgtt tcggggtgct
      661 gcggcaagtt ggctgaaaaa atcacgggaa aagcaattga aaaggccgtg gaggagtctt
      721 ccggcggtga tgccagcctt tcgctggacg gtaatatcga tatctcggat attccagccg
      781 cttttcggta tccgggtatg aaaggggtag cgcgcatcgc ccagaataca tccacatcca
      841 cgggaactgt ctatatgctg gaatccgaag attccttgga aaaagtcaag acccattatg
      901 aagccattgc gggctacaaa cagatttcga agctggaaca gccaggaatc gatccagttt
      961 ttacttatga aacggcgcca aaggaaagct gcatcatcca gctttcgaca aggagcgcca
     1021 aaacggcgat catcctcacc ttcgcgaagg aaaaaacgta agttcgggcg aaacgtgagt
     1081 tgggggggtg gttggatcat gggcgaacga tggcggcaaa ttttccatcg tttgaaatgc
     1141 ggctgcgatg gtttcgtggc ttgaggtttc ccgcggcatc tctgtgcaat agacgcctgt
     1201 cgaacgagcg gcgcatggtc gtgggcagcg acgagggcgt ttgccattcg gctcttccct
     1261 tgcgaacgtt ggtttgccat attccctcgc tcccatgcca atcattgcaa aaaaaataga
     1321 gcgcgacgaa cgagaggaat gtctttaccg gatccgtcat agcacggcgc atctgatggc
     1381 cgaggccgtc caggttcttt ttccgggaac gaagctggcg tttggacctc cggtacaaga
     1441 tggtttttat tatgattttg actccgacca tcgttttacg gacgaggatc tgaagacgat
     1501 cgaaagcaag atgcgggagt tggcgaagaa gaagtcgcct ttcgtgaaaa aatcgatatc
     1561 gaaggccgaa gcgcttcgtc ttttcgagca gcaaggcgag aaactcaaag ccgagcatgt
     1621 gcagacgctt tcggatggtg agatcacgtt atacgaaagc ggtgggttcg tggatttgtg
     1681 cgcggggcct cacgtggaga gcacggcgca gctcgagcat ttcaagttga cgaacgtatc
     1741 gggtagttac tggcggggcg atgagaagcg cgagcgattg cagcggatat acggaaccgc
     1801 ctgggattcg aaacaagcgc tgaaagcgca tctggctcgc atcgaggagg ccaaaaagcg
     1861 ggaccatcgg gttttgggca aggagcttgg gctattcgag tttccggaac tcgcggggcc
     1921 cggtcttccg ttttatcttc ccaaaggcgc gcgggtgctg cagcaactca aagactggat
     1981 gtggcaattg cacatcacgg gcggctacgg tcatccggac aagacgtacg aaccgctggc
     2041 cacgcctcat atccttcgga cggacgcctg gaagatcagc ggacacctcg agaattaccg
     2101 agaaaacatg tttatggtct acagcctcga cgaactcgat caggggcttt tgacgcggga
     2161 gtcgcgagag gattcgcaga cgaacgacaa ggatgggctg ggcaattacg ggttgaagcc
     2221 gatgaattgt ccggggcatg tgatgttgtt caacgtgggg accaagagtt atcgcgactt
     2281 gccggcgcgg tacttcgagt tcggaaccgt gtatcgttat gaaagagcgg gcacgttaca
     2341 tggcatgctt cgcgtgcgca gtttcaccca agacgacgcg catctattct gcacgcctga
     2401 caatgtggct cgggaaatcg aaggggtatt cgatttttgc tgccatatcc tcgacacctt
     2461 tgggttcgat tatcacgtgg gtctgaaaac acgtcccgag agccgactgg gtacggacga
     2521 gatttgggac atggcggaaa acggattgcg tgccgtgctc gaaaagcggg cggcgggcaa
     2581 gtactacgtg caagaaggag atgcgacgtt ttacggtccc aagatcgact ttacgattcg
     2641 ggattgtctc ggtcgtgaat ggcaaggggg cacgatacaa ctcgatttca atctgcccga
     2701 gcgcttcgaa ctcgagtata cggatgcgga taatacgcgc aaacgacccg tcatgatcca
     2761 tcgcgcgatt ctgggctctt tcgaacggtt tttcggcctt ctcatcgaag aattcggtgg
     2821 agccttcccc ttgtggctcg cccccatcca cgtgcgcgtc ctgcccatcg gcgataccca
     2881 gctctcgtac gcccagtccg tggtcggtga attgaaacgc cgcggcttcc gcgccgaact
     2941 cgatgattcc aacgacaaac tcggcaaaaa aatccgaaac ggcaagatgg ataaagttcc
     3001 ctatctcgtc gttgtcggac agaaggaagt cgacgacaag acgatcacca cggaaagcta
     3061 tcacgatggg aaactcgaag acgtgacgac cgtcgatgcc ctttccgatc gaatgaacga
     3121 tgagatcgag cgtcgcgtgt ttcgtcgtcg cgcgtgagcg aggtggagat cagaactcgg
     3181 ctgggtcgct gtaggaaccg aaaactgcgc ggaacacgtc gcagatttcc tgttccgtac
     3241 aataggcttt ggcggcctcg atgatcgagg gcatgagatt tttggttccc tgggcggccg
     3301 tgcggatttc ttccaggcat tgtttgactt gcgattcatc gcgagaagcc ttgacgcgag
     3361 ccagggcgtc gcattgcgcg cgctgcacgc tttcgtcgat gcgaagcaaa ggtatcttgg
     3421 catcctcgtg ttgcgtgtat ttgttgacgc ccaccatgac ccgttcgcct gcttcgagct
     3481 gacgttggaa gcggtatgcg gaagcggcga tttcgcgttg gggatagcct ttttccacgg
     3541 cttccaccat gcctcccagc gaatcgatgt gttcgatgat ggccatcgcg tctttttcga
     3601 gcttgtgggt caaccattcc acgaaataac tgcctgcgag tgggtcgatg gtgcccgtga
     3661 cgccgctttc ttccgcgatg atttgctggg ttcgaagggc aatcgtggcg gcctcctcgg
     3721 tgggaagcgc ataggtttcg tcgagggaat tggtgtgcaa cgattgtgtg ccaccgagca
     3781 cggcggccag ggcttgaagg gccacgcgca cgacgttgtt ataaggttgt tgtgccgtga
     3841 gggccacgcc tgcggtttgt gcgtgggtgc gtagtttcag gctggctcgg ctcttggcgc
     3901 cgaaccgatc gcgcatgaat cgcgcccaca tgcgtcgtgc cgcgcggaac ttcgcgattt
     3961 cttcgaaaaa gtcgttgtgg acgtcaaaga aaaacgaaag gcgcggcgaa aaagcatcca
     4021 catccatgcc ccgttcgagg gcatggcgca cataagcgag cccatcggcc aaggtaaagg
     4081 caagctcttg tgccgccgtc gcaccggctt cgcgaatatg atagccgctg atcgaaaccg
     4141 tattccatcg aggcacttca ctggcacaaa actctatgga atccgcgacc atacgcatgg
     4201 ctggccgcgg tggcaccaac cacgcgtgct gggcaatgaa ctccttcaaa caatcgtttt
     4261 gtaccgtccc accgatcttc gatcgggcga tgccccgctt gtccgccaac gcaatataaa
     4321 aagccaacaa cacgatcgca ggcccattga tcgtcatcga cgtggtcacg cgatcgaggg
     4381 gaatgccgtc gaatagcacc tccatatcgc gcaacgagtc gacggcgaca ccgcacatgc
     4441 caacttcgcc cagggcgcgt ggggaatcgg agtcgtagcc catgagggtg gggaagtcga
     4501 acgcggtaga aagtccggtt tgtccttggc tgagcaggaa cttgaaccgc tggttggttt
     4561 gttcgggggt gccgaagccg gcgaacatgc gcatggtcca taggcgaccg cgatacatgg
     4621 tgggctggac tccacgagta aaagggaaca tgccgggcat gcccaagtca tgggcataat
     4681 cgatgggggc atcgagcggc gtggccacat cgggaagatc gacatcgctc caggtcgtaa
     4741 atcgctcttt tcgcaaggga atacggtcaa tcgtggcggc gacgggtccg tttcgccatg
     4801 ctgcaagctg ttcacgaaga gcctcgacgc ctgctgcgtc caaacccaaa acctcgcctt
     4861 cagccgattc tgtgatggtt tccttctgct cgttacccat attgtgctct cccgtatcgc
     4921 cgaagcccgt tcgatgctag cacgtgtgaa atagcatagg tcgagggttc gaatcccgtt
     4981 tggtaggcta aatcgtcatg cgacccgagg tcggacaaat catcgccggc aagtatcgtc
     5041 tcacccgtct catcggagag gggggaatgg gaagcgtttt cgaggcaaac cacgaatact
     5101 tgggcagcgc ggtggccttg aagtttctca accccatcct tgcgtcacga ccggctttgg
     5161 tggctcgctt tcttcaagag gcgcgtgttt ctgccagcat tcgaagtcct catgtcgtgc
     5221 aagtctccga tgtagatcag acgttggagg gattgcccta tctggtgatg gaactgctgg
     5281 agggcgaaag cttgcagacg tgcatgaacc gttgtcggac gttgcccttg ggcacggcct
     5341 tggaatacgc ggtccagatt ctcaacggtt tggagagtgc tcatggtcgt ggagtcgttc
     5401 atcgggactt gaagccggac aatgtgttca tcgtgccgac accgcacggt gctttgctca
     5461 agcttttgga ctttgggatc gcgaaacttc gtagcattgc tgatgagcaa ccgggattga
     5521 cacgaccggg ttcggtgatg ggtacgttgg agtacatggc gccagaacaa gccttatcgg
     5581 cggatcgagc ggaccttcgg gccgacttat actcggtggg agtcatcctg tttgaaatga
     5641 tcgcgggcgt tcgtcccctc gaggccgatg atccacaaac gatggctgct caagtgatgg
     5701 cggggcagat tcgacggctg aacgatatcg tttccggaat tccccaaccc ctttcggaca
     5761 cgattgctcg ggcgttggcg ggccagccgg cccaacggtt tcaaagcgcc gcggagatgc
     5821 gaacggcgtt gctgcccttt ttcacggcat cggttcaagc cgtaggcatg ccccattacc
     5881 aaagcatggt cgccctaccg gcggcggcgc ctgctcgttc tcctacggcg gtgctggcag
     5941 acccggccat atcggcatcg gatcgtccgg ctcccacgca accgggaacc gatcccggtc
     6001 agccggctgg agtgtcgcca accctgccac catcggaatc cgcggagttg gtggctggac
     6061 gaacgggaac cgtgcttggc gatgcggcag gcgcatttcc cgaaggattt ggcgctacgg
     6121 cacagatgca gcccatgg
//
LOCUS       DAOSOR010000342         6128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_12942_length_6128_cov_4.097975, whole genome shotgun sequence.
ACCESSION   DAOSOR010000342 DAOSOR010000000
VERSION     DAOSOR010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12942_length_6128_cov_4.097975"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PK710_16610"
     CDS             complement(<1..365)
                     /locus_tag="PK710_16610"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOT11406.1"
                     /translation="MSDGRRVWVVKSLDALDGQSHPALVMDLFVVLQRCKPFAHAPEA
                     SVRDLARVATTKELRRHETLWNAGEMPAAFCTIHSGLFKIVRTLSNGREAIMGLFGPR
                     ESIGDVAVIQGIGYPATPVV"
     gene            complement(362..1807)
                     /locus_tag="PK710_16615"
     CDS             complement(362..1807)
                     /locus_tag="PK710_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11407.1"
                     /translation="MRRFPSLALVIYSLISIGCGSEGGGADPAPLPDAHNEPPPDASS
                     EAAADSGVDALPESAIDVASDTVTDTLTDTVTDPPVDSPADTANDTPSDVPLDLPPDT
                     PTDVPIDTPTDSPVDAPIDAQIPTIALLVASDYSTTAEMIAVDVQQQTTLGHLLSDDQ
                     DSVVDANNGLGFLLRRTVDEVQVLDSAQPWVVTHTIDVTSGAGHSNPWAVVVPAHDKA
                     YVVRYGSNVLSIINPSTGANLGEIDLSAYVIDSDGLVDAFGGVFDPSRGRVYIGLQRI
                     DQLEWGTAPDYVHTCNDSHPAIVGIDTLTDSIIDLNGTEDGEALQLSSFNPWRMIWDD
                     VSERIVLLSIGCASVDDGGISIRQRRGIEAIEPITGISSWLWTSEALDRPADLIWISN
                     QEAVVGIDDASFTRTWYAWNPSTTQLGPALSGVPNVPLWDGARGLVGLVFGSTGLDVV
                     RYDLDEGTTSMVVENAYNTPSVFPASSVRLR"
     regulatory      complement(1909..2110)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2280..3971
                     /locus_tag="PK710_16620"
     CDS             2280..3971
                     /locus_tag="PK710_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing serine/threonine-protein
                     kinase"
                     /protein_id="HOT11408.1"
                     /translation="MTGSSRPPDPVSKGTIINERYEIRQSLGKGGMGEVFLAYDGSTQ
                     QMVALKLVREDSRMPGDDEALRQELLHARSVGHPNVCRVHDLAPSPWGPILVMEHIQG
                     QTLHAHIRRKKPHGGYTVDEFRKIASESSAGLAAIHSQGLVHGDLKPGNVMVTDDRAI
                     ILDFGFAQERARVYARRPGAPPDGGTPNYMAPERLRSGGASPEDDVYALGLTLWEMWT
                     CRVPEPGSRPRARRMREQIMFDIPSQLCVDETKQIFRCLSENPKMRPQAKHLRFFNPA
                     RFTVSPVQVPRERLDPGPPLGRAAVNQFVPGTQSLLVTFASNSPDIVGRLFPLNTPSL
                     TIGRRTDQDIALPEPTVSGTHVNVRWSAGTWTFDDLGSTNGTYADHSYERKAQAVLMH
                     GGEVQLGELRLKLVSFGVDSPHLQRAQQFLTHRDGLTGLLSRESLLEALDEEGAFCEW
                     AELPLTIARYELRGPNRQVSERPTILELLALRRAAQRCVELTETLMLALSPVVSGRSG
                     PLRFAIAMAGPSVEESRTLVEQVVSQVQNTIPDRIELFATIVKGEPGRPARTLID"
     gene            3971..4786
                     /locus_tag="PK710_16625"
     CDS             3971..4786
                     /locus_tag="PK710_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT11409.1"
                     /translation="MPSIDRKNTKPRDDHLVVRNVIKRFGDVVVLDDVSMVIRRGQTA
                     VIIGGSGAGKTTLLRILIGLEKPSSGSVFIDGEDIVPLGGLAMNRVRKKFGMVFQYSA
                     LLDSLSVMDNVAFPLVEHTRMKKAAIRERVLSTLESLGLQTSDAQKSPAELSGGMRKR
                     VALARALMLEPEILIYDEPTSGLDPITSRMVDDLIEETRERYGITSVVISHDMASTFR
                     IAHQAFLLIRGKVAASGTPEELADINRNKVSHDFIVASGVAFERISRPPLPGD"
     gene            complement(4837..5439)
                     /locus_tag="PK710_16630"
     CDS             complement(4837..5439)
                     /locus_tag="PK710_16630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11410.1"
                     /translation="MRFSHLLTVSLFAIIGLGGCGGSGWNAQSPAGVSPAPSIVDASP
                     SLQVLATIERFRQLDPAIDGFLKNAYGFAVFPAVGSRSGSQGAYGEGEVYEQNELVGK
                     AILTHGEVGEPEYSELIFFQDRRPLDEFRNGSFQFSSRASHEASSHDNYDQGVAVFAI
                     TKRGFQNGPAVTEQKFDFEPVEDGGRLPLSAAWLDSSSVL"
     gene            5579..>6128
                     /locus_tag="PK710_16635"
     CDS             5579..>6128
                     /locus_tag="PK710_16635"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOT11411.1"
                     /translation="MNVSVDSLLFDPSALAIHIANVLLESTLQTEESRDEVMSRSAVL
                     FLLQDAPRCAPEEGPRLVLTRRSKNVPQSGDLCCPGGGMENVDRWLAKLVDAPGLMLR
                     HWPHHEKLPSRTRHAVATLVATALREGFEEMGIRPWNVRLLGLLPPQSLRMFRRAIQP
                     VVVSLVGHPHFRPNWEVADIIAI"
BASE COUNT         1326 a         1720 c         1765 g         1317 t
ORIGIN      
        1 acgacgggtg ttgcgggata tccgattcct tgtatcaccg cgacgtcacc gatgctttcg
       61 cgggggccga acaatcccat gatcgcttcg cgaccgttgg ataacgtgcg aacgattttg
      121 aacaaaccgc tgtggatcgt acaaaatgcc gcgggcatct ctcccgcgtt ccaaagcgtt
      181 tcatgccgac ggagttcttt cgtcgtggcc acgcgggcca agtcgcgaac cgatgcttcc
      241 ggcgcgtgag caaacggctt gcaacgttgt aaaacgacga aaagatccat gaccagagca
      301 gggtgcgact gaccgtccaa cgcgtcaagg gacttgacga cccagacgcg tcgcccatca
      361 ctcaacgcaa tcgaacgctc gaagcgggga atacgcttgg cgtattgtag gcgttttcca
      421 ccaccatcga tgtcgtgcct tcgtccagat cgtatcggac cacgtcgagc cccgtgctgc
      481 caaaaacaag acccacgagc cctcgtgccc catcccacaa cggcacgttc ggaacgcccg
      541 aaagcgccgg gcctagctgc gttgtcgacg gattccaggc gtaccatgtt cgtgtgaaac
      601 tagcgtcatc gatgcccacg accgcttctt gattcgatat ccaaatcaga tccgcgggac
      661 gatccagagc ctccgaagtc catagccacg acgaaatgcc tgtgatcggt tcgatagcct
      721 cgatgccgcg tcgctgtcga atggatatgc caccatcgtc cacggaagca cagccaatgc
      781 tgagcaatac aatccgttcc gaaacgtcat cccagatcat tcgccaagga ttgaaggatg
      841 agagttgtag tgcttctcca tcttcagtcc cattgaggtc gatgatcgaa tcggtgaggg
      901 tgtcgatacc gacgatggct ggatgggaat cattgcacgt gtggacataa tcgggggcag
      961 tgccccattc gagctggtcg attcgctgca gtccgatata cacacgccca cgggaaggat
     1021 cgaatacccc gccaaaggcg tccacaagcc cgtcgctgtc gatgacatac gcggaaagat
     1081 cgatctctcc aaggtttgct cccgtcgaag gattgatgat gctcaacacg ttggagccgt
     1141 atcgaacgac gtacgccttg tcgtgagcgg gtaccacgac ggcccaaggg ttgctgtgtc
     1201 cggcccccga tgtcacatcg atcgtatgcg tgacaaccca aggctgggcc gaatcgagca
     1261 cctgcacttc gtccaccgtg cgacgaagca ggaatcccag gccgttattg gcatcgacca
     1321 cggaatcctg gtcatcggaa agcagatggc caagtgtggt ttgttgctgg acgtcgacag
     1381 cgatcatttc cgcggttgtc gagtagtcgg aagccacaag cagagcgatg gtaggaattt
     1441 gagcatcgat gggagcatcg acggggctat ccgttggggt atcgataggg acgtctgtgg
     1501 gcgtatctgg cgggaggtct aggggaacat cggaaggcgt gtcgttcgcg gtatcggcgg
     1561 gtgaatcgac agggggatcg gtgacggtat cggtgagggt gtcggtgacg gtatcggaag
     1621 cgacgtcgat ggcgctttcg ggtaaggcat cgacaccgct atctgcggcg gcttctgagg
     1681 atgcatctgg gggtggttcg ttgtgggcat cggggagggg agcggggtcg gcacctcctc
     1741 cttctgaacc gcaacctatt gaaattagtg aataaattac cagagcaaga gaggggaaac
     1801 ggcgcatgat cgagaacctc cgcgcgcaca cccgcgtgcg ctttgcttgg cgacgccttg
     1861 ggggaggagg tttccgacct gtctccagag accccgtgga gccgcgcaac atcggttacg
     1921 acgcgaggca ggtctcctgg cttccggatc atccttgggc gaacccttcc cagacataaa
     1981 aatccagtgg tttcgttcgc ttttgtcccc ggtcacagtg gcgggggccg cgccggaatg
     2041 acaccggctt ccctcttgca accgctgttt tcctaagcga aaatcagcga cctcgcggct
     2101 gggtcaaccc gaagtggtta cccagcgcgg tgcctaacgt gcggcgaggc tgtcgtcaag
     2161 ggattcatcc tccatttgat gatatctttc acttttcatc ggattgttga cccaaactgc
     2221 gtcgtcgcaa ggtaggcttg ccaccaaggc gcgagattag agactgcttg gtccagaaca
     2281 tgactggttc atcacgaccg ccagatcccg tatcgaaggg caccatcatc aacgaacggt
     2341 acgagatccg tcaaagcctt ggcaaaggtg gcatgggtga ggtgtttctt gcctacgatg
     2401 ggagcacgca gcagatggtg gctctcaaac tggtgcgaga agactcacgc atgccgggag
     2461 acgatgaagc gcttcggcaa gagctgctcc atgcccgttc ggtggggcat ccgaatgtat
     2521 gccgtgtcca cgacctagcc cccagtccat gggggcccat cctcgtcatg gaacacattc
     2581 aaggacagac gctccacgca catattcgcc gaaaaaaacc gcacggcggc tataccgtgg
     2641 atgaattccg aaaaatcgcc agtgagtcga gtgcggggct cgccgccatc cattcccaag
     2701 ggctcgtgca tggggatctc aaaccgggca acgtcatggt taccgacgat cgagcgatca
     2761 ttctcgactt cggtttcgcc caagagcgtg cgcgcgtgta tgcccggcgt cctggtgctc
     2821 ctcccgacgg tggaacaccg aactacatgg cgcctgaacg attacgttcg ggaggcgcga
     2881 gtccggaaga tgatgtctac gcactcggtt tgaccctttg ggaaatgtgg acgtgccgtg
     2941 ttcctgaacc tgggtcacgt ccgcgcgctc gacgcatgcg agaacagatc atgttcgaca
     3001 tcccatcgca actctgcgtc gatgaaacca agcaaatctt ccgttgtttg tccgaaaacc
     3061 cgaagatgcg tccgcaagcg aagcatcttc gttttttcaa tcccgcgcgc tttacggtga
     3121 gcccggtgca agtgccgcga gaacgattgg atcccggtcc ccctctcggc cgcgctgccg
     3181 tcaatcaatt cgttcccggt acccaatctc tgctggttac ctttgcttcc aattctcccg
     3241 acatcgtggg tcgtctattt cccttgaaca ctccatcgct gaccatcggt cgacgcaccg
     3301 atcaggatat tgcgttgcct gaaccgacgg tttccgggac tcatgtcaat gtgcggtgga
     3361 gcgcgggaac gtggacgttc gacgatcttg gtagcaccaa tggcacctac gccgatcatt
     3421 cctacgagcg aaaagcgcaa gctgtgctca tgcatggtgg tgaagtacaa cttggggaac
     3481 ttcgtctcaa gctcgtgagc tttggtgtcg actcgcctca tctgcagcga gcgcagcaat
     3541 tccttactca tcgcgatggc cttaccggct tgctcagccg cgagtcattg ctcgaagcgc
     3601 tcgatgagga gggcgctttc tgcgaatggg ctgaactgcc gttgaccatc gcgcgttacg
     3661 agcttcgagg tccgaaccgg caggtaagcg aacgtccgac catcctcgaa ttgctagcgc
     3721 tgcgacgggc ggcgcaacgt tgtgtcgaac tcactgaaac gttgatgctt gcactcagtc
     3781 ccgttgtttc gggacgtagt gggcctcttc gatttgcgat tgccatggct ggaccttccg
     3841 tagaggaatc cagaaccctg gtcgagcaag tcgtctcgca agtacaaaac acgattcccg
     3901 accgcatcga gctttttgcc acgatcgtaa aaggcgaacc cggacgcccc gcgcgcacgc
     3961 tcatcgattg atgccatcca tcgaccggaa aaacaccaaa cctcgagacg atcacctcgt
     4021 cgttcgcaat gtcatcaaac gattcgggga tgtggtggtg ttggatgacg tgtcgatggt
     4081 cattcgaagg ggacagaccg cggtcatcat tggcggttct ggtgctggca aaacgacctt
     4141 attgcgcatc ctcatcggtt tggaaaagcc ttcttccggt tctgtgttca ttgatggcga
     4201 ggatatcgtg ccgctgggag ggctcgcgat gaatcgcgtg cgcaagaagt ttggcatggt
     4261 gttccaatac agcgcgttgc tcgactcgct ttctgtcatg gataacgttg cttttccgtt
     4321 ggtggaacat acacgaatga aaaaggcggc cattcgggag cgagtcctga gtacactcga
     4381 atcgctcgga ttgcagacgt cggatgcaca aaaatcccct gcagaactat ccggcggcat
     4441 gcgaaaacgc gtggcgctgg ctcgagcctt gatgctcgaa cccgagatcc tcatctacga
     4501 cgaacccacg agcggactcg atccgattac ttcacgcatg gtcgatgatc tcatcgaaga
     4561 gacgagagaa aggtatggca tcaccagtgt cgtcatctcc cacgacatgg cgagcacctt
     4621 tcgaatcgcc caccaagcat tccttctcat tcgaggcaag gttgccgcgt caggaacccc
     4681 ggaagaactg gccgatatca atcgaaacaa ggtgtcgcac gatttcatcg tcgcttcggg
     4741 ggtggctttc gaacgtattt cacgaccgcc ccttcccggc gactaacagc cgctcgttgt
     4801 gttttgggcc aacagggctt cctgcgaacg gtcaccttac aagacagaag agctatcgag
     4861 ccaagcggcg gacaagggca gacggccacc atcttcgacc ggttcgaaat cgaatttctg
     4921 ttccgttacc gccggaccgt tttggaaacc gcgtttggtg atggcgaata cagcgacacc
     4981 ctgatcatag ttatcgtggg aagacgcttc gtgggaggcg cggctgctga attggaagga
     5041 gccgttgcgg aattcatcca acggtcgtcg gtcttggaaa aaaatcagtt cggaatactc
     5101 gggttcgccg acctctccgt gggtaaggat tgctttgcca acgagttcat tctgttcgta
     5161 gacttctccc tcaccgtagg ctccttggga gccggaacgg gaaccgacgg ctggaaacac
     5221 cgcgaatcca taagcatttt tcaagaaacc atcgatggcg gggtccagtt ggcgaaaccg
     5281 ttcgatggta gcaagaactt gaaggctcgg ggaggcgtcg acaatcgatg gggctggtga
     5341 tacgccggca gggctttgag cgttccagcc ggatccgccg catccgccca agccgataat
     5401 cgcgaacagt gaaacagtga gaaggtgcga aaagcgcatc aattccctca ttcaaaacaa
     5461 gaaggtgtct tgccataatg caagccgcag gccaagtaat acgcattccc cgatgattcc
     5521 agttgattgt tgagatctgc ggattttacg gtttacaacc cgatcaagcc ccaaactgat
     5581 gaacgtttct gttgactccc tacttttcga tccatctgcc ctggcgattc acattgcgaa
     5641 cgtgcttttg gaatcgacac tgcagacgga agagagccgg gacgaggtga tgagtcggtc
     5701 ggcggtgttg tttttattac aagacgcgcc ccgatgtgcg cccgaggaag ggccgcgcct
     5761 cgtattgacc cgaagatcga aaaatgtgcc tcaatccggg gacttatgct gccccggggg
     5821 cggaatggaa aacgtcgatc gatggcttgc caagctcgtg gatgcccccg gcctgatgtt
     5881 gcgtcattgg ccgcaccacg agaaactgcc ttcccgaacg cgccatgcgg tggcaacact
     5941 cgtggccaca gcgctgcgtg aaggattcga ggaaatgggt attcggccgt ggaacgtgcg
     6001 tttgttgggg cttttgccgc cgcaaagcct tcgcatgttt cgacgagcca tccaacccgt
     6061 tgtcgtttct ttggtgggac acccccattt tcgccccaat tgggaggtcg ccgacatcat
     6121 cgccatcc
//
LOCUS       DAOSOR010000343         6110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_12998_length_6110_cov_4.195376, whole genome shotgun sequence.
ACCESSION   DAOSOR010000343 DAOSOR010000000
VERSION     DAOSOR010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12998_length_6110_cov_4.195376"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PK710_16640"
     CDS             <1..559
                     /locus_tag="PK710_16640"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT11412.1"
                     /translation="GALHFFIDRFEASRPDATATDPGWMETRRCVNSGVYPWTYANYA
                     EAEAACAKTGHRLCTAQEYRVACESAAGHLYPYGNVYEPMTCNGLDYDGVPGGANDNV
                     LIPTGNLLQCVAPGSIFDLSGNAAEWTSSVTSNTGPPTNLDIHVVMGGSYQTPALGLS
                     CGFDLSRFAENAILPELGFRCCKDP"
     gene            664..1425
                     /locus_tag="PK710_16645"
     CDS             664..1425
                     /locus_tag="PK710_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11413.1"
                     /translation="MAQHLSPRSPGRLLRAALWGIVLLVPGVALAATVKGQVKGHNEL
                     INPVWEESARPDTNRYTWREPSPTVRSDYRRLFGLAPKELCIAGVSKNKVEPPKVPIL
                     VLVGGGRTTPVTLVVAPGTRLRFRNNDPFKHRLYAVNEASFSPADIAMAAERDWTAPG
                     PGTYELRDELAPSLRSWIVVKEGVASIGYPNRQGQFFLTLEPGEYQLEYFFAGKPIGT
                     PRPVSVGGQDLDITREPIQVALPKDKNASGDAEKK"
     gene            1435..3162
                     /locus_tag="PK710_16650"
     CDS             1435..3162
                     /locus_tag="PK710_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 family protein"
                     /protein_id="HOT11414.1"
                     /translation="MLLSRFWYLILALALGLFASGLYLAQTVYNRASRDSMEQALSAD
                     TQVVKWYLNDDARRRSSLLLKVCLDETIRKELHKASSKDEKVPQDVRDRTRAALRKLV
                     DEMPAEYQFSSLFAIDQLGRVIGQVGFDQAAGIENFDMGGYAIVADALHGWVRDDAWV
                     FDDRIYLVVARPVEVDTAQMPAGAVVGLRIVDTSYAKQLSKRSGATVGFFAGGTVKAK
                     ASPDGVSSTQLDTIVADIKNLAQDDTYKEKGTSKPKILMDDIGVIYSKIPGEAWDLGA
                     GYVVGRSASVVKNPFDLLQLADAKEKKSQPWWIIALIVVLGAGLGLVFSLFEHTRPLR
                     ILKAEAERLAKGEIDMLQVSKFRGLYRKIASDLNDGTDKVAAKGGVPRKAADLESVLG
                     PIPAQPEMSAFSFPVDGASPSTSGPLRVPPLRESAPKMPVQMPAPKPSPDSKPQMPAP
                     KPDSPKMPAPKPADAMTPNLAPSEDDDDATVVSAVPPEILEASATGAHAAVEEAGEIA
                     EWHNTFEEFLRTKKQCGEPTSGLTFEKFQVTLRKNREQLIAKTKCKRVKFTVYVKDGR
                     AALKASPVR"
     gene            3193..4746
                     /locus_tag="PK710_16655"
     CDS             3193..4746
                     /locus_tag="PK710_16655"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOT11415.1"
                     /translation="MQVTRCPSVVCIAAALSLALVSSRTDASTGYEFPENGTEQLGRA
                     GAWTARASNSLATFFNPAGLAGQPSSLLLNSSLIWQKNCLKRTYPDGNPVRLGNGGAY
                     QYPNEVCNDDSGTPFPNPQLAGVLRLSDRLGLGLAVLGPSTRGQFTFPSHVTGSRVGG
                     VEREVPSPQRYLLQSDELLFIWPQIGIGWEPIDDLRFGASLIWGIARLKFSILAAGRS
                     AGETIQNYPYLAEQYLDFQADIDVKDLFVPGFTVGTLWSPSPNIDVAAWYHWMDDIRA
                     KGDMAVEGSVYGDPPHQRPSSRNSVRAPKDSVQVVAPWPMQARVGFRWHQPRDGIHSK
                     MRDPIANDVWDIELDMTWANNSHFKYLDATMPDGVFLEVHNPADPNPNSSVAFAMPTD
                     ARVPHHWKDSFGFRLGGDYVMLPNRLAARAGAFFDSEAQDPKYLNVDYVPSRKFGLHV
                     GGTVRFGALDLMVAYAHVFYQGLDNQGDGKTQGLAGAAPTYRTDYPVNGGSNRSVINA
                     LSFGASHSF"
     gene            4773..5840
                     /locus_tag="PK710_16660"
     CDS             4773..5840
                     /locus_tag="PK710_16660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HOT11416.1"
                     /translation="MRVLYGVVGEGMGHATRSKVVIAHLLENNHRVRIVVSGRAYHFL
                     KKHFDDVVEIQGLSITYDDGAMDRDRSVLDNMMRSPAIVVGNAAAYFERVVDFAPQLV
                     ISDFDSFAYLYAKAHATPVISIDNQQIIHRCEHEEDIRKGVDFDFFSTKAFVKAKLPG
                     CDHYFITSFFFPKIRGKHAKNTTLVPPILRPEILNASSSRAKHILVYQTSVSDTSLIP
                     TLRRLSDHQFIIYGLRRNEVQGNCIIKDFSEDQFIRDLSSARAVVSNGGLSLIHEAIY
                     LRKPVFSVPIRHQFEQEMNARYLEKLGYGLGASKIEFDVLSWFLQQEDRYFDALGNHH
                     QDGNRMLFEKLDEVLDRFDRP"
BASE COUNT         1370 a         1814 c         1588 g         1338 t
ORIGIN      
        1 aggggcgctt cactttttca tcgaccgttt cgaggcgtcg cgtccagacg ctacggccac
       61 cgaccccggc tggatggaaa cccgtagatg tgtaaatagt ggtgtatatc catggactta
      121 cgccaattat gccgaggccg aagccgcttg cgcaaagacg ggacaccgat tgtgtaccgc
      181 gcaggaatat cgagtggcgt gtgagagtgc ggcggggcat ctataccctt acggcaacgt
      241 ttacgagccg atgacgtgca atgggcttga ttatgatggg gtgcctggag gggcgaatga
      301 caacgttttg attccgacag ggaatctcct tcaatgtgtg gcgccaggtt cgattttcga
      361 tctttctggg aatgcggcgg aatggacaag ctcggtgacg tcgaacaccg gaccgcccac
      421 gaacctggac attcatgtgg tcatgggtgg gtcttatcag acgccagctc ttggtttgtc
      481 ctgcggtttc gatctcagcc ggtttgcgga aaacgcaatc ttgcccgagc tcggctttcg
      541 atgctgcaaa gacccgtgat atcctgcctg caacaagaac gatcggtttt gtggccaatg
      601 cgctaggatg ccgttggtca ttcgctcagc cctgcggatt tcatctcgaa catgtgcaaa
      661 tcaatggctc aacatctttc ccctcggagc cccgggcgtc tgctgcgagc agcactctgg
      721 ggtattgtct tgctcgtccc aggcgtggct ttggcggcaa ccgtcaaagg tcaggtcaag
      781 gggcataacg aactcatcaa tccggtttgg gaggagtccg cgcgaccgga taccaaccgc
      841 tatacgtgga gggagccttc gcccacggtc cgttcggatt atcgaaggct ttttggtcta
      901 gcaccgaagg aactatgcat tgcgggggtg agcaaaaaca aagtagagcc tcccaaagtt
      961 ccgattctcg tgcttgtggg agggggtcga accacacccg ttacgttggt ggtagcgccc
     1021 ggaacacgcc ttcgttttcg caacaacgac cctttcaaac accgtcttta tgcagtgaac
     1081 gaagcctctt tttctccggc ggacatcgcg atggctgcgg agcgagactg gacggcgccg
     1141 ggaccaggaa cgtatgagct tcgcgacgag ctggcgccta gtcttcgctc ttggatcgtg
     1201 gtcaaagagg gggtcgcctc catcggatat ccaaaccgac aaggacagtt tttcctcacc
     1261 ctcgaacctg gtgaatacca gctcgaatac ttcttcgcgg gaaaaccgat cggtacgcct
     1321 cgtcctgtat ccgtgggagg gcaggacctc gacatcaccc gcgaacccat ccaagttgcc
     1381 cttcccaaag acaaaaacgc gtccggtgac gcggagaaaa aatagggagg ccgaatgctt
     1441 ctctcccgat tttggtatct catcctcgct ctggctctgg gacttttcgc cagtggcttg
     1501 tacctcgccc agaccgtcta caaccgcgct agccgcgact ccatggaaca agcactctcc
     1561 gccgataccc aagtcgtcaa gtggtatctc aatgacgacg cgcgtcgacg ctcgagtttg
     1621 ctgctcaagg tttgtctcga cgaaaccatt cgaaaagaac tccacaaagc cagctcaaaa
     1681 gacgaaaaag tcccccaaga cgttcgggac cgtacacgcg cggcactcag aaaactcgtc
     1741 gacgaaatgc ctgccgagta tcagttctcc tcgctgtttg cgattgatca actcggacgt
     1801 gtcatcggtc aggttggctt cgatcaagct gctggaatcg aaaacttcga tatgggtggg
     1861 tatgccatcg ttgccgatgc gctccatggt tgggtacgcg atgatgcatg ggttttcgat
     1921 gaccgtatct atcttgtcgt agctcgcccc gtcgaagtgg ataccgcgca aatgcccgct
     1981 ggtgcggttg tcggtcttcg aatcgtggac acgagttatg caaaacaatt gtccaaacgc
     2041 tctggcgcga cggttggctt tttcgcgggc ggtactgtca aagccaaagc atcgccggac
     2101 ggggtcagtt cgacacagct cgatacgatc gtggcggaca tcaaaaatct ggcgcaagac
     2161 gatacctaca aggagaaagg aacgagcaaa cccaaaatcc tcatggacga tatcggggtg
     2221 atttattcca agatcccagg agaagcgtgg gacctggggg ccggttatgt ggtgggccgc
     2281 tcggcgagtg tcgtcaaaaa tcctttcgat ctgcttcaac ttgccgacgc aaaagagaaa
     2341 aagagccagc cttggtggat catcgctttg attgtcgtgc tgggggctgg actcggtctc
     2401 gttttctctc ttttcgaaca cactcggcct cttcgaattc tcaaggctga agccgaacgc
     2461 ctggccaagg gcgagatcga catgcttcaa gtcagcaaat tcagaggatt gtaccgaaaa
     2521 atcgcaagcg acctgaatga tgggacagac aaggtggcgg ccaaaggcgg tgtcccacga
     2581 aaagccgccg acctcgaatc ggtgcttgga cccatcccag ctcagcccga aatgagtgcg
     2641 ttttcattcc cggtcgatgg cgcttccccg tccacatctg ggcctcttcg tgtgcctccg
     2701 ctccgtgaat cggccccaaa aatgcccgtg caaatgcctg ctcccaaacc atcaccggac
     2761 agcaagcccc aaatgccggc tccaaagcca gactcaccga aaatgcctgc gccaaaaccg
     2821 gccgacgcga tgactcccaa cctagccccc tccgaggatg atgacgatgc taccgtcgtt
     2881 tccgcggtcc ctcccgaaat cctcgaagcc tccgcgaccg gtgcccatgc cgcggtggaa
     2941 gaagccggtg agatcgccga atggcacaac accttcgagg aattcctccg aaccaaaaaa
     3001 caatgtggag agcccacctc cgggctgacc ttcgaaaagt ttcaggtcac gctccgaaaa
     3061 aatcgagagc aactcattgc caaaaccaag tgtaaacgcg tcaaattcac ggtctacgtc
     3121 aaggatggga gggctgccct caaagctagc cccgttcgat aagcaaccct ggctccccaa
     3181 aggagtgtcc ccatgcaggt gactcgatgt ccttcggtgg tttgcatcgc tgctgcgtta
     3241 tctctcgcgc ttgtttcgag tcgtacggat gcttcgacgg gctacgaatt ccccgaaaac
     3301 ggaaccgaac agcttggccg tgccggcgcg tggaccgcgc gcgcttccaa ctctctcgcc
     3361 acgtttttca atcccgccgg cctcgccggc cagccttcca gcctgctgct caattcttcc
     3421 ttgatctggc aaaaaaattg tctgaaacgc acctaccccg acggcaaccc cgttcgtctg
     3481 ggaaatggtg gcgcctatca ataccccaac gaagtctgca acgacgatag cggtacccct
     3541 ttccctaacc cgcaactcgc gggagtccta cgattgtccg accgtcttgg cttgggcctc
     3601 gccgtcctcg gcccctcgac ccgcggccaa ttcacgttcc catcccatgt cacgggctct
     3661 cgagtcggtg gagtcgaacg agaagtcccg agcccccaac gctatctgct gcaaagcgac
     3721 gaactattgt tcatatggcc ccaaatcggt atcggttggg aacccatcga tgatttgcgc
     3781 ttcggtgctt cgttgatctg gggcatcgct cggctgaagt tctccatcct cgctgccgga
     3841 cgatccgccg gcgaaaccat tcaaaactac ccatacctcg cggaacaata cctcgatttc
     3901 caagccgata tcgatgtcaa agacctgttc gttccgggat tcaccgtcgg cacgttatgg
     3961 tcgccctcgc caaacattga cgtggctgct tggtaccact ggatggacga tatccgcgcc
     4021 aaaggagata tggctgtcga aggaagtgtc tacggagacc cgccccatca acgcccaagc
     4081 tcacgaaact ccgtgcgtgc tcccaaagac agcgtgcagg tcgttgcgcc atggccgatg
     4141 caggcccgag ttggttttcg atggcaccaa cctagagacg gaatccattc gaaaatgcgg
     4201 gatcccatcg ccaacgacgt ttgggatatc gaactcgata tgacgtgggc caacaactcg
     4261 cacttcaaat atctcgacgc caccatgccc gacggcgtct ttctcgaagt gcataacccc
     4321 gcggatccca atccgaattc cagtgtcgcc ttcgccatgc cgaccgatgc gcgcgtccca
     4381 caccattgga aagactcctt cggttttcgt ctcggcggcg actacgtcat gttgcccaat
     4441 agactggccg cacgtgccgg cgccttcttc gattccgaag cgcaagaccc caaatacctg
     4501 aacgtcgatt acgtcccctc tcgcaagttc ggtctgcatg tggggggaac cgtccggttt
     4561 ggagccctcg acctgatggt ggcttatgcg cacgtctttt atcaaggcct cgacaaccaa
     4621 ggggacggca aaacccaagg cctggctggc gctgccccca cgtatcgcac agactatcca
     4681 gtcaatggcg gttccaatcg ttccgtcatc aatgcgctat cgtttggagc ttctcactcg
     4741 ttctgacgtt tcacatcgaa tccggaaaag ccatgcgtgt tttatacggt gtcgttggcg
     4801 aaggcatggg gcatgccacg cgatccaaag tggtcatcgc ccaccttctg gaaaacaatc
     4861 atcgcgttcg catcgtcgtg tccgggcgcg cttaccactt cttgaaaaaa cacttcgacg
     4921 atgtcgtaga aatacaagga ctttccatta cttacgacga tggagccatg gaccgtgacc
     4981 ggtccgtgct cgataacatg atgcgctcgc cagccattgt cgttggcaac gccgcggcgt
     5041 acttcgaacg agtcgtggac ttcgcaccgc aactcgtcat cagcgacttc gactcctttg
     5101 cttaccttta cgccaaagct catgccacgc ccgtcatatc catcgacaat caacaaatca
     5161 tccaccgctg cgagcacgag gaagacattc gaaaaggcgt cgacttcgat tttttctcca
     5221 ccaaagcttt cgtcaaagca aagctgccag gctgtgatca ctacttcatt acatccttct
     5281 ttttccccaa gatccgaggg aagcacgcga aaaataccac cctggttccg cctatccttc
     5341 gcccggaaat cctcaacgct tcatcatcac gcgcaaaaca catcctcgtt taccaaacct
     5401 ccgtctcgga tacgtcgctc atcccaaccc tccgtcgcct ttccgaccac caattcatca
     5461 tctacggcct gcgacgaaac gaggtgcaag gcaattgcat catcaaagac ttcagcgaag
     5521 atcagttcat ccgtgacctg tcatcggctc gcgcggtcgt atccaatggc ggcttgtcgc
     5581 tgattcacga agccatctac ttgcgaaaac ccgtcttcag cgtgcccatc cgccaccaat
     5641 tcgaacagga aatgaacgcg cgctacctcg aaaaattagg ctatggcctg ggagcctcga
     5701 agatcgagtt cgacgtttta tcttggtttc tccagcaaga agaccggtac ttcgatgctc
     5761 ttggcaacca ccaccaagac ggcaatcgaa tgcttttcga gaagcttgac gaggtgctgg
     5821 accggttcga cagaccttga tgaaccggtg ggacgagtgc ttcgtcgttc gacgagctta
     5881 tcgcctagca agagtcgaaa tgctgtaaat ctatgttgtt tcttgattat tgattgatct
     5941 tctcgactgg gcagacgcta gcgtggaaag ctgaaccttc tgcgcgaagg tcgcatcatt
     6001 gcggaaaacc gacgaaggaa agggtaacct ttcgacatga ccgaggcgcc atcgccactt
     6061 tctttggctc acccgaacac ggactacgat gtcgttgtga ttggaggtgg
//
LOCUS       DAOSOR010000344         6110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_12999_length_6110_cov_4.147977, whole genome shotgun sequence.
ACCESSION   DAOSOR010000344 DAOSOR010000000
VERSION     DAOSOR010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12999_length_6110_cov_4.147977"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PK710_16665"
     CDS             <1..386
                     /locus_tag="PK710_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT11417.1"
                     /translation="FEFSKATLLPESEPVLEQLKLFLEQKTQITLVRIEGHTDNVGQP
                     AANLKLSGERALAVRTWLVNKGIDGKRLIAVGFGDLKPVADNKTDEGRAQNRRTEFIV
                     VEVGNKPWLGADTTAGGTVFGENPR"
     gene            451..807
                     /locus_tag="PK710_16670"
     CDS             451..807
                     /locus_tag="PK710_16670"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOT11418.1"
                     /translation="MTRWIVPSLFLTLFIGCTHDSAKDKSTSEGGDSQPITETEAANL
                     YSIRCASCHGAEGKGDSPLGRTLHLSDLSTREWQEKTSDKDIEKVIVQGRGQMPAFKL
                     SEPERKALVKTIRSFR"
     gene            812..1204
                     /locus_tag="PK710_16675"
     CDS             812..1204
                     /locus_tag="PK710_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11419.1"
                     /translation="MSLSDRFPRFRRLAPLILILAAVVVYFEFVSPHGTEEHTLVFQF
                     EDSPDQVTQIDTAWSELDSRESEVVGGASLYFKLGQAPRQVVTKIHAPRGTYWLDITV
                     TRGSDRSALRQRVSLQGNAKIFIPATKP"
     gene            complement(1359..2249)
                     /locus_tag="PK710_16680"
     CDS             complement(1359..2249)
                     /locus_tag="PK710_16680"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT11420.1"
                     /translation="MKQRHSIFLLCASVLAGCVDDRSPALSHDAPAALGVAPRETTTA
                     VPLMLAALGPQVIGNVLTSPSLSDAMAAAAGRTASAKQQDSACPKEMVLVSGQYCPEV
                     EHKCVRWLETEGRYAYFRCAEYARPAKCLRERQSMRFCIDRDEYIPPGESLPAADQSW
                     TDAKRICESLGKRVCQESEWNFACEGEEMRPYPYGFVRDAEACNADRIDIYRSNGRLS
                     DLREGPDGHPGCTSPFGVHHLSGNLEEWTTIDGTSPARPAMKGAYWQPSRNHCRAAQT
                     AHDEYYHGDETGFRCCKDVD"
     gene            complement(2392..3210)
                     /locus_tag="PK710_16685"
     CDS             complement(2392..3210)
                     /locus_tag="PK710_16685"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOT11421.1"
                     /translation="MSSRLQQPIIAIANQKGGVGKTTIAVNLAAGMVRRGRRVLLIDL
                     DIQGSASAILGRSEGEPDGNVAKCLLEGRPLSEAIIPTAYDGLDLAPAGERMAIVDLH
                     LASALGREHVLARAFRSVAIADYDLVLLDTAPYLGLLTLNAIVASTHLLVPVSCEYLP
                     MLGLKLLRDTLGSVQQQIGIDVPILGYVLTMIDRRERITDEVEAMLRKTFGNQVFEHG
                     IRINTHHKASPSHRQTIYDYEPAGGRGRADFERLCDEVEVRLGLSRKAPAANPA"
     gene            3826..4005
                     /locus_tag="PK710_16690"
     CDS             3826..4005
                     /locus_tag="PK710_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11422.1"
                     /translation="MKLAPWFMAALAPFAVIGFGNSMIAHLAVMMLTLGIFFGTLSLG
                     KSRVSQNAQRSGDPT"
     gene            4090..5178
                     /locus_tag="PK710_16695"
     CDS             4090..5178
                     /locus_tag="PK710_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04552 family protein"
                     /protein_id="HOT11423.1"
                     /translation="MVENAGQFKRIEEFDLFDVETVKLILRGESVVDWHRLNLATEQR
                     AVELLEAMEYHPAEPADRARLDKVKYEAISFLRRHFDFPIPKPVERASVEELMLIASG
                     KGHRQLCACTILKAMHIVHHVECRELAFSLPMSMQDVFKLVEEKVYRVIGDMLAAGFP
                     ITEFIGGRKNKDSLYTKLLSKTETTAANVYDRIRFRIVTRSADDILPTLLYLSKRVFP
                     FNQVIPGQSLNTMFPLKSYLSQLESVRGFVPLLQLGIGDDLTQSGNTFSGKAFRAVHF
                     VADVPVRLPREHLDNAPHAWALGPVVSVLSEFQVVDTDTEVTNELGDGSHSAYKSRQK
                     QAVMRRLKLGTQGSSRKSDTPPRSRRRG"
     gene            complement(5111..>6110)
                     /locus_tag="PK710_16700"
     CDS             complement(5111..>6110)
                     /locus_tag="PK710_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11424.1"
                     /translation="LARHAFAQSEQAYHRALADMAEQVRPTLAAAKEFSPFLEPFERM
                     EQQLENRFQALEQRNLEKQENTAVVDREIEALHDELNRKQADTQAAAMKLDQQKERLQ
                     RIEVAFRRAEIELRNAQTVARAAAGPEAKVALPQDAQKIRDLTSVLEERRQALEAPRA
                     EVEQAQRVWNDADLELKAVQRRMAELQKKRRLIEQSYTKEIAIRTEGVAQAQLERQTA
                     LVALGSALLEGKPQLVPQALQQEIQTVRDTMARHEREQARLDNALTLADDEAVKKGWI
                     ILAAAVGIAFVLLVFLIAWLGRSEPPIGMNTSEAVNLAFVNGEAYRFFGNYPGFPA"
BASE COUNT         1278 a         1669 c         1784 g         1379 t
ORIGIN      
        1 tcttcgagtt cagtaaagca accctgcttc cggaatccga gcccgtgctc gaacagctca
       61 agctattcct cgagcaaaaa acgcaaatca ccctcgttcg catcgaaggt catacggaca
      121 acgtcggcca gcccgctgcc aatctcaaac tctccggtga acgagccctc gccgtccgaa
      181 cctggctcgt caacaaaggc atcgacggca aacgcctcat cgccgtcggc ttcggtgacc
      241 tgaagcccgt cgcggacaac aaaaccgacg aaggccgcgc acaaaaccga cgcaccgaat
      301 tcatcgtcgt ggaagttggc aacaagccct ggctcggtgc cgataccacc gccggcggaa
      361 cggtcttcgg cgagaatccc cgctgatccc tttccgtttt catctcccaa atcactgggt
      421 ccctcttggc aatcttgcta acgtggcgag atgaccagat ggattgtccc ttccctattc
      481 cttaccctat tcatcggttg cacccacgat tcagccaagg ataagtccac ctctgaaggc
      541 ggggactccc agcccatcac cgaaaccgaa gctgcaaacc tttattcgat ccgatgcgcc
      601 tcatgccacg gagcagaggg aaaaggcgat agccccctgg gaagaaccct tcacctttcg
      661 gacttatcca ctcgcgaatg gcaagaaaaa acatccgaca aggatatcga aaaagtcatt
      721 gtccaaggac gtggacagat gccagcgttc aagctctcgg aaccggaacg caaagctctc
      781 gtgaaaacaa tccgttcctt ccgttgatac gatgtctctt tcggaccgtt ttcctcgctt
      841 tcgacgcctt gcgccgctga ttctcatcct tgcggccgtg gttgtgtatt tcgaattcgt
      901 ttctcctcat ggcaccgagg agcatactct cgtttttcaa ttcgaggact cgccggatca
      961 ggtcacgcag atcgacacgg cgtggtcgga actcgactcg cgggaaagcg aggtagtcgg
     1021 tggtgcttcc ttatatttca agctcggaca ggcaccccgg caagtagtca ccaaaattca
     1081 cgcccctcgc gggacctact ggctcgatat cacggtaact cgcggttccg atcgatccgc
     1141 cttgcgacag cgcgtatccc tgcaaggaaa tgccaaaata ttcatccctg ccaccaagcc
     1201 atgaaggcca aggtcccttt ggcgcagggc tggacaatcc cccatccgat aggtcctgcc
     1261 tgaaaaccga agggtcgata gcacgaaccc ctctgcgaga cgaaaagcgc gagtcaccgc
     1321 tctagctgtc gctcggcctg gatgggcccc caaacgcgtc aatccacgtc tttgcagcag
     1381 cgaaagccgg tttcatcacc gtggtagtat tcgtcgtggg ctgtttgtgc cgctcggcag
     1441 tgattgcggg aaggctgcca ataggcacct ttcatcgcgg ggcgagcggg gctggtgccg
     1501 tcgatggtgg tccactcttc caggttgcca gaaaggtggt ggactccaaa aggactggta
     1561 caaccggggt ggccgtcggg gccctcccgc aggtcactca agcggccatt ggagcgatag
     1621 atatcgatac ggtcggcgtt gcaggcctcg gcatcccgca cgaagccgta gggatacgga
     1681 cgcatttcct cgccttcgca tgcgaaattc cactcggatt cctggcagac ccgtttgccc
     1741 aagctctcgc aaattcgttt ggcgtccgtc caggactgat cggccgcggg taaggactca
     1801 ccggggggga tgtattcatc gcggtcgatg caaaaccgca tggattgacg ttcacgcaag
     1861 catttggcgg ggcgtgcgta ttcggcgcag cggaagtagg cgtagcgccc ttccgtttcc
     1921 aaccaacgaa cgcatttatg ctccacctcc gggcagtatt ggccggaaac gagaaccatt
     1981 tctttggggc aagccgaatc ttgttgtttg gcactggccg tacgcccggc agcggcggcc
     2041 atcgcatcgg aaagcgaggg agaagtgagg acgttaccga tcacctgcgg gccgagggca
     2101 gcgagcatca acggcacggc ggtcgttgtc tcacggggtg ctacacccaa cgcggctggt
     2161 gcgtcatgcg acaaagccgg gctacgatcg tcaacacatc ctgcgagtac ggaagcacag
     2221 agcaggaaga tggaatggcg ctgcttcatg ggctttctgc cgagattagg ccgaaatgtc
     2281 agggatttgc cacggcgtca agcaacaacg gctcattttt tgaaaataat ttgtatatat
     2341 ttgaatttag ggtgtttttt agccacctgg gagcaagggg ggggcgtcga atcaggcggg
     2401 gtttgcggcc ggtgccttgc gtgaaagccc caagcgaacc tccacttcat cgcatagtcg
     2461 ctcgaaatcg gccctgccac gcccgcccgc gggttcataa tcataaatgg tctgacggtg
     2521 gctcgggctg gccttgtgat gggtattgat gcgaatccca tgctcgaaaa cctggtttcc
     2581 aaaggttttc cgaagcatcg cctcgacttc gtcggtgatg cgctcccgac ggtcgatcat
     2641 ggtcaagacg tagccgagaa tggggacatc gatgccgatt tgctgttgta cggaaccgag
     2701 ggtatctcgg agcaatttca gtcccagcat gggcaagtat tcacaggaga cggggaccaa
     2761 cagatgggtc gatgccacga tggcgttgag ggtgagcaag ccgaggtacg gggcggtatc
     2821 gagtaaaacc aggtcataat cggcgatggc gacggagcgg aaggcgcgcg cgagcacgtg
     2881 ttccctgccg agagcggaag ccaggtgcaa gtcgacgatg gccattcgtt cgccggcggg
     2941 agccaagtcc aggccatcgt aggcggttgg aatgatggct tctgacaagg ggcgcccttc
     3001 gagcaaacac ttggcgacat tcccgtccgg ttccccctcg gagcgtccaa ggatcgcgct
     3061 tgcggatcct tgaatatcga gatcgataag caaaacacga cggcctcttc ggaccatgcc
     3121 cgcggcgaga ttgacagcaa tcgtggtctt gcccacgcct cccttctggt tcgcaatggc
     3181 gatgatgggt tgctgcaagc gactggacat gacacctccc ttattgaagt ggtcgttagc
     3241 gcatcagaca cggggcagaa ggtcaaggat caagtcgggg gcaacgcaag gcccctggtc
     3301 tcgaagatta tttcgagcgt cgaaatggag gcgccacgac tcttcaaacc caaatattta
     3361 ctaattattt caacaggttg ctacgattag gggagccttg tttcgggctg ggtgggagga
     3421 ctttcgaaac cgatgctttt cattcaccgt tgacagaaag aaaaagtcca tggatcgcca
     3481 tcatcgacgg ttccgacccc tggtcagggg tgtggggatc agcccgcagc gagcggtaag
     3541 gtagccacaa tttcccaaag ggacggacgt cgaaactgac gtaaccaatt gaatttacgt
     3601 tgatcacaga aaaggtggcg gggttggcat cctaggcgca tcgttttttt cttgaaggcc
     3661 gtatatctcg tcgctaagag ttggcccata tcagagatag gtagtatctt atcctacata
     3721 tacggaactt gcttttgctt tttcgagata cgaccggatg ttccggttcg accgtgtggt
     3781 gagaccaatg acgaaggtcg ctgaagcagg cgacatggag gaacgatgaa gctggcccca
     3841 tggttcatgg cagcgctcgc ccccttcgcg gtgattggat tcggcaattc gatgattgcc
     3901 cacctggcag tgatgatgct cacgctaggc atctttttcg gcacgttatc cctcggcaag
     3961 tcgcgtgtca gccaaaacgc tcaacggtcg ggagatccca cctgatcgtc gggagcctca
     4021 ctttttatcc ttttttcaaa ccgaagaatt gtctacgtgt gacagaccta ccggggtagg
     4081 gtttgtggca tggtggaaaa cgcgggtcag ttcaagcgaa tcgaagagtt cgatctgttc
     4141 gacgtggaaa ccgtcaagct gatccttcgc ggcgagtcgg tggtggactg gcatcgcttg
     4201 aatttggcga cggagcagcg ggcggtcgaa ttgctcgagg ccatggagta tcaccctgcg
     4261 gagccggctg atcgggcgcg tctggataag gtcaagtatg aggccatcag ttttttgcga
     4321 aggcatttcg attttccgat ccccaaaccg gtcgagcgtg cctccgtcga ggagttgatg
     4381 ctcattgcgt cgggcaaagg gcatcggcag ctatgtgcgt gtacgattct caaggccatg
     4441 cacatcgtcc atcacgtgga gtgtcgggag cttgcgtttt cgcttccgat gtcgatgcaa
     4501 gacgtgttca agctggtgga agagaaggta tatcgagtga ttggggacat gctggcggcg
     4561 ggttttccga tcacggaatt cattgggggg cgaaaaaaca aggattcact ctatacgaag
     4621 cttctttcca agacggaaac aacggctgca aacgtctacg accgcattcg atttcggatt
     4681 gtgacgcgca gtgccgacga tattttgccg actttgttgt acttatcgaa gcgggtgttc
     4741 ccgttcaatc aggtgattcc ggggcagagt ttgaatacga tgttcccgct caagagctat
     4801 ttatcccagc tcgagtcggt tcgtggtttt gtgcccctgc ttcagcttgg gattggcgac
     4861 gatctcacgc aatccggcaa tacatttagc ggcaaagcgt ttcgtgccgt tcatttcgtg
     4921 gcggatgtgc ccgtgcggct tccgcgcgag catctggaca acgcgcctca cgcttgggcc
     4981 ttggggccag tcgtttcggt gctcagcgag tttcaagtcg tggatacgga tacggaagtg
     5041 accaacgagc tgggcgatgg cagccattcc gcttacaaaa gccgtcaaaa gcaggctgtg
     5101 atgcgcaggc tcaagctggg aacccagggt agttcccgaa aaagcgatac gcctccccgt
     5161 tcacgaaggc gaggttgacc gcttccgacg tgttcatccc tatcggtggc tcggagcgtc
     5221 ccagccacgc gatcaaaaaa accagcaaga caaatgcgat accgacggcc gcagccagga
     5281 tgatccagcc tttttttacg gcctcatcgt cggccaaggt cagtgcgttg tcgagacgcg
     5341 cttgttccct ttcgtggcgt gccatggtgt ctcggacggt ttggatttct tgttgaagtg
     5401 cttgcggaac gagttgtggt ttgccttcca gcagggccga tccgagggcg accaacgcgg
     5461 tttgtcgctc gagttgcgct tgggcaacgc cctcggttcg aatggcgatt tctttggtat
     5521 aggactgctc gatgaggcgg cgtttttttt ggagttcagc catgcggcgt tgcacggctt
     5581 tgagttccag atcggcgtca ttccacacgc gttgggcttg ttctacctct gcgcgaggag
     5641 cctcgagagc ctgtcgtcgt tcctcgagga ccgatgtcaa atcacgtatt ttttgagcgt
     5701 cctgaggcaa cgcgactttg gcttcggggc cggctgcggc ccttgccact gtctgcgcgt
     5761 tacgcaattc gatctcggcg cgtcgaaatg ccacctcgat cctttgtagc cgctcttttt
     5821 gctgatccag cttcatggct gccgcttggg tatcggcctg cttgcgattc agctcgtcgt
     5881 gaagcgcttc gatctcgcgg tcgacgacag cggtattttc ctgtttttca aggttccgct
     5941 gttcaagcgc ctggaaacgg ttttccagtt gttgttccat gcgctcgaac ggttcgagaa
     6001 agggcgagaa ttctttggcc gcagcgaggg tgggtcggac ttgctcggcc atgtcggcca
     6061 gggcccgatg ataggcttgt tcgctttggg cgaaggcgtg ccttgccaaa
//
LOCUS       DAOSOR010000345         6097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13039_length_6097_cov_4.837637, whole genome shotgun sequence.
ACCESSION   DAOSOR010000345 DAOSOR010000000
VERSION     DAOSOR010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13039_length_6097_cov_4.837637"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PK710_16705"
     CDS             <1..462
                     /locus_tag="PK710_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT11425.1"
                     /translation="LLIEVVTDNRNRTVAELRKIFEKHNGSLGSSGTAAWAFDRKGVF
                     QVDGATDEGKLMDVAVGAGADDYENTGEVWQVVTPVELFDTVAKALEADGITVLESGL
                     EFLPKSKKSLVGRDAEVALNLTDALDDHDDVQNVFADLDVSDEEMARISGE"
     gene            587..2440
                     /gene="dnaG"
                     /locus_tag="PK710_16710"
     CDS             587..2440
                     /gene="dnaG"
                     /locus_tag="PK710_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOT11426.1"
                     /translation="MISPETIAQVRQRANIVAIISETVKLTRRGRHHLGLCPFHKEKT
                     PSFHVNGETGRYYCFGCHEKGSVIDFIMRLEGLEFPQAVRVLAERIGIEIEELSAEKT
                     ASSTSRETTRTKQRLYEINQIAASFFETQLRQHPFSRVAREELKRRELVAATPTDAVA
                     DALQAFRVGYAPAGWDGLLSHLRGQGVSPTDAELLGLIVPRKGGSGHYDFFRNRLMFA
                     VIDVQGRVVGFSGRLLPDPQTGDIDKQTGKYVNSPESPIYKKSETVFGLFQARQAIRQ
                     RELAVVVEGNFDVLSLHARGINYAVAPLGTAFTEAQARVIRRFTPRATLVFDGDKAGR
                     AAIAKSRMVCAMAGLDTRVACLPTGLDPDDFVRQRGSPALERLIEQAQSILEYLIEQV
                     LDESFPQSNPHEQASRVQWVAQLIRDESDPIVRAMAENYADKVVARLGLSDARTVRAL
                     HSRLRQAARQPTLSASTPRAPRKSDVGEFKLSLEMLGCIIEFPELLDDDEISKHIEIV
                     EGEIVHVFKLVHDVHNLPKSDWQDSFLAHLQPSIHDFAAQRLAAPRIDDAAQAKEEFL
                     KNAQKLKRLHLAREHAQVVKEIHQAGAQGDIQEENELLLEAVRKQRERLGL"
     gene            2594..4396
                     /gene="rpoD"
                     /locus_tag="PK710_16715"
     CDS             2594..4396
                     /gene="rpoD"
                     /locus_tag="PK710_16715"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOT11427.1"
                     /translation="MRGAQPAKTKEPAKPKGSKEVQQLIDLGRSKGFLTYEEVNDALP
                     QDVVAPDQIDDVISALGDEDIQIVDVTTQVKLGSKRMTDQEPDDGKKVRVREDEPEED
                     LDAYYSRSSDPVRMYLRKMGSVSLLTREGEVEIAKRIEHGELMVLDAILNSPIAVREI
                     IELGDKLRRHKIRVKDIIRDAEDDEQGFDEEEADRRIIRLIDKVKRLDKKHQDLLAAA
                     KAESDGKKSQLREEIKTTRHELVKTLEEMRLNKKTIDKIVGRLKRLIQKVEHAESSAA
                     ELEKRTGVSRSQIASLVQSAKQNPANERKVARKLGVTRAELAEIESTLRNAQSGVRKV
                     EEELDIIVEEVRACYSAIRRGEQVAERAKAELVEANLRLVVSIAKKYTNRGLQFLDLI
                     QEGNIGLMKAVDKFEYKRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKL
                     IRTSRYLIQEYGREPTPEEIAVKMELPIDKVRKVLKIAKEPISLETPIGEEEDSHLGD
                     FLEDKNAVSPSEAVINLNLSDQTRRVLKSLTPREEKVLRMRFGIGEKSDHTLEEVGQD
                     FEVTRERIRQIEAKALRKLRHPSRSKQLKSFVDT"
     gene            4492..5613
                     /locus_tag="PK710_16720"
     CDS             4492..5613
                     /locus_tag="PK710_16720"
                     /inference="COORDINATES: protein motif:HMM:NF019240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1571 domain-containing protein"
                     /protein_id="HOT11428.1"
                     /translation="MNDAPGAPVVCYGHEVPSFSNVFPKLLRLTLELLSIDQIRLTID
                     ALSEPQRAAMLHFLGENGLASVVETLDTSKQDLVKASVAHLPDSTHQHFSTVYPNIML
                     RLYDTLTPEQIITFMKRLDAKDLAAQSLFMGNEGRKIVFSGLRDDSVAAVLDKTEDWV
                     FLETGKRALANLAQYECTLIKQERVGKKLQDPETIHLKMREDPKAIYMKWIAGPFKGR
                     ELLYSTKLGTDVMRVREAGVFGIVPVTIGLDTPVARRGTNHRVTEVGFRHLLALIEKD
                     YAVAAPRGHVQRVNHGLETVHGRKTYKMETILPRDPRLGYYCYRMMHWTDFVRGIEVK
                     SEVYNFQDELQEAYDYKDVNDAPGFSDLDFDPHNKAYKL"
     gene            complement(5654..>6097)
                     /locus_tag="PK710_16725"
     CDS             complement(5654..>6097)
                     /locus_tag="PK710_16725"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT11429.1"
                     /translation="EGEDMLPYATGFERDPSKCNIDKEWRKKNRELLPYGECMKNKAC
                     AAEFAKLDQRHAIGERTTCVSPFGIYDLNGNVNEWVFVPWGKAPFRGAIKGGWWGPVR
                     SRCRPIVTSHDEKYTGYEVGFRCCKDVAGAAPTSSQSPASDAATP"
BASE COUNT         1565 a         1715 c         1607 g         1210 t
ORIGIN      
        1 ttgctcatcg aggttgtcac cgataatcga aaccgcacgg tagccgaact gcgaaaaata
       61 ttcgaaaaac acaacggttc tttggggtct tcgggaaccg cggcgtgggc tttcgaccga
      121 aagggtgtgt tccaagtcga tggcgctacc gacgaaggca agctcatgga cgtggccgtg
      181 ggagcgggag ccgacgacta tgaaaacacg ggggaagtat ggcaggtggt gactcccgtc
      241 gagcttttcg ataccgtggc gaaagccctg gaagccgacg gcatcaccgt cttggaaagc
      301 gggctcgaat tcttgcccaa gagcaaaaaa tccctggttg gaagggatgc ggaggttgcc
      361 ctcaacctca cggatgcgtt ggacgatcac gatgacgttc aaaacgtttt cgccgatctc
      421 gatgtctccg atgaggagat ggcccgtatt tccggcgaat gaacaacaat tgacaagatc
      481 atgacattcc gtggcgcgag ggtggtgttg gcgctacggt atggttttgc atgaattcag
      541 tttcatgact gggtgtgatg aaaggacctg accgagagcc ttttggatga tttcgcccga
      601 aacgatcgcc caggtgcgtc agcgcgccaa tatcgttgcc atcatcagcg agacggtgaa
      661 acttacgcga cgcgggcggc atcaccttgg gttatgccct tttcacaaag aaaaaacgcc
      721 gagtttccat gtcaatgggg aaacgggacg gtattattgt ttcggctgcc atgaaaaagg
      781 cagcgtgatc gattttatca tgaggctcga aggactcgag ttcccgcaag ccgtccgggt
      841 ccttgccgaa cgaatcggta tcgaaatcga ggaattgtcc gcggaaaaga cggcatcatc
      901 cacgagccga gaaacgacac gtaccaaaca acgcttgtac gaaatcaacc agattgcagc
      961 gtcgtttttc gaaacccaat tgcgacaaca ccctttctcg cgggtggcgc gtgaggagtt
     1021 gaagcgacgg gaactggttg ccgcaacccc cacggacgct gttgctgatg cgcttcaggc
     1081 atttcgcgtg gggtatgctc ccgcaggatg ggatgggttg ctgtctcatc tgcgcgggca
     1141 gggagtgtcc cccacggatg ctgaattgct cggccttatc gtgccgcgaa aaggcggaag
     1201 cggacactac gacttttttc gcaatcgatt gatgttcgcc gtcatcgacg tgcaggggcg
     1261 tgtggtggga tttagtggtc ggctgctgcc ggacccccaa accggcgata tcgacaaaca
     1321 aacgggcaaa tacgtcaatt cacccgaatc gcccatctac aaaaaaagcg aaacggtatt
     1381 tggccttttt caagcacgac aagccatccg ccaacgcgag cttgccgtcg tggtggaagg
     1441 caacttcgac gttttgtctt tgcatgcgcg aggcatcaat tatgcggtgg caccgttggg
     1501 tactgctttc acagaagcac aggcccgtgt catccgtcgg ttcacgccgc gagccacctt
     1561 ggtattcgat ggcgataagg cggggagagc agcgattgca aaaagccgaa tggtatgtgc
     1621 catggctggg ttggatacgc gggtagcttg cttacccacc ggattggacc cggacgactt
     1681 cgtaagacag cgtggttccc ccgcgttgga acgtctcatc gaacaggccc aaagtatcct
     1741 cgaatacctc atcgagcagg tcctggacga aagctttccg caaagcaatc cgcatgaaca
     1801 ggcctcccgg gttcagtggg tagctcaact cattcgcgat gaatccgacc ccatcgttcg
     1861 tgccatggcc gaaaactatg cggacaaggt tgtcgcgcgc ttgggcctga gtgatgcccg
     1921 aaccgtccga gcgttacact cacgattgcg ccaggcggct cgtcaaccga cgctctcggc
     1981 atcgacccca cgagcgccgc ggaagtcaga cgtgggagaa ttcaagcttt ctttggaaat
     2041 gctggggtgt attatagaat ttccagaatt attggatgat gatgaaatta gtaaacatat
     2101 agaaattgtg gaaggcgaga tcgttcacgt gttcaaattg gtacatgatg tgcataacct
     2161 gccgaaatcc gattggcaag actctttcct tgcacatctg cagccttcga tccatgactt
     2221 cgcggcccag cgtctcgccg ctcctcgtat cgacgatgcg gcacaggcga aggaagagtt
     2281 tcttaaaaat gcccagaagt tgaagcggct gcatcttgcg cgggagcatg ctcaggtcgt
     2341 caaagagatt catcaagcgg gggcccaggg tgacatccaa gaagaaaatg aattgctcct
     2401 cgaggccgtc cgcaagcaaa gggagcgatt ggggctttga cgactggatg accgaggcca
     2461 ttgattgtcg ataacgaggg agccggtgcg agcgaaaaaa tccgatcgaa agagtaaaga
     2521 ccaccacgcg gtgtcggact tctccactca ccctcggagc catgccaagc tccgcgcgga
     2581 ttccggcaag ccgatgcgag gtgctcaacc cgccaaaacc aaggagcctg ccaaacccaa
     2641 ggggagcaaa gaagtccaac agctcatcga tctcggacgc tccaaaggct ttcttaccta
     2701 tgaagaagtc aatgatgcgt taccacaaga cgtggtggcc cccgatcaga tcgatgacgt
     2761 catctcggct cttggggacg aagacatcca gatcgtggat gtgacgacgc aggtcaagct
     2821 tgggtcgaaa cgcatgaccg atcaggagcc tgacgacggc aaaaaagtgc gtgttcgcga
     2881 ggacgagccg gaagaggacc tcgatgccta ttattcgcgt tcgagcgatc cggtgcgcat
     2941 gtacctgcga aaaatgggta gcgtatcgtt gctcacgcgg gaaggcgaag tcgaaatcgc
     3001 caagcgaatc gaacacggag aattgatggt cctcgatgcc atcctgaact ccccgatcgc
     3061 cgtgcgcgag atcatcgaac ttggtgacaa actccgtaga cacaaaatcc gtgtcaagga
     3121 catcatccgg gacgcggaag acgatgagca gggcttcgat gaagaggaag ccgaccgtcg
     3181 tatcattcgg ctcatcgaca aagtcaaacg attggacaaa aaacaccaag atctattggc
     3241 tgccgcaaaa gccgagtccg atggcaaaaa aagccagctc cgcgaagaga tcaaaacgac
     3301 acgccatgaa ctcgtcaaaa ctctagaaga aatgcgtctg aacaaaaaaa cgatcgacaa
     3361 gatcgtcgga cgcctcaagc ggctgattca aaaagtcgaa catgcggaat cgagcgctgc
     3421 cgaactcgaa aaacgaaccg gtgtgtctcg tagtcaaatc gcatccctgg ttcagtccgc
     3481 caaacaaaac cctgccaacg aacgaaaagt agctcgaaaa ctaggcgtaa ctcgcgctga
     3541 acttgccgag atcgaatcca cgttgcgaaa cgctcagtcc ggcgtccgaa aagtcgaaga
     3601 agaactcgat atcatcgtcg aagaggtccg cgcttgctac tccgccatcc gtcgtggcga
     3661 acaagtcgct gaacgagcca aggcagaact cgtcgaagcc aacctccggc tggtcgtttc
     3721 catcgcaaaa aaatacacca accgcggact gcaattcctc gatctcatcc aagaaggcaa
     3781 catcggattg atgaaagccg tggataagtt cgaatacaaa cgcggctaca aattctcgac
     3841 ctacgccacc tggtggatcc ggcaagccat cacgcgcgcc attgccgacc aagcccgtac
     3901 catccggatc ccggtccaca tgatcgagac catcaacaaa ctcatccgaa cctcgcgcta
     3961 tctcatccag gaatacggac gagaacccac accggaagag atcgccgtca agatggaact
     4021 gcccatcgat aaagtgcgaa aagtccttaa aatcgccaag gaacccatct ccctcgaaac
     4081 tcccatcggt gaagaagaag attcgcatct cggtgatttt ctcgaagaca aaaatgcggt
     4141 cagcccttcg gaggccgtca tcaatctgaa cctgagcgac caaacccgca gagtcctcaa
     4201 atcattgacc ccacgagaag aaaaagtgct ccgcatgcgc tttggtatcg gagaaaaaag
     4261 cgaccatacc ctcgaagaag tcggccaaga cttcgaagtc acccgggaac gcatccgaca
     4321 aatcgaggca aaagccctgc gaaaacttcg tcatccaagc cgctccaaac agctcaaaag
     4381 cttcgtcgat acctgaacac ccgcccgcgc ccgcatccct atccaatacc tttccagcca
     4441 tggctcgccc tgagccttga cgcccgcgtg gcgtacggtt tggatcttcc catgaacgat
     4501 gctccaggtg cccctgtcgt ctgctacggc cacgaagttc cctcgttttc caatgtcttt
     4561 cccaaactcc tgcggctcac tctcgaactg ctttccatcg accaaatacg cctcaccatc
     4621 gatgccttgt ccgaacctca gcgcgccgcc atgcttcact tcctcggaga aaatggcctc
     4681 gcgagcgtcg tcgaaaccct cgacacctcc aaacaagatc tcgtgaaagc aagtgtcgca
     4741 cacctgcccg actcgactca tcagcacttc tccaccgtgt acccgaacat catgcttcgg
     4801 ctctacgaca cgctcacacc cgaacaaatc atcaccttca tgaagcgcct cgatgccaaa
     4861 gacctcgccg cccaaagctt gtttatgggc aatgaaggcc gaaaaatcgt gttttccggg
     4921 ttacgagacg atagcgtcgc agccgtgttg gacaaaaccg aagactgggt gtttctcgaa
     4981 actggcaaac gcgccttggc gaatcttgcg caatacgaat gcaccttgat caaacaagaa
     5041 cgcgttggaa aaaaactcca ggatccggaa accattcacc tgaaaatgag ggaagatccc
     5101 aaagcgatct acatgaaatg gatcgccggt ccgttcaaag gacgagaact cttgtacagc
     5161 accaaacttg gaaccgatgt gatgcgagtc cgagaagcag gcgttttcgg tatcgttccc
     5221 gtcaccatcg gattggatac ccccgtggca agacgcggaa cgaatcatcg agttaccgag
     5281 gttggatttc gacacctgtt ggcgctcatc gaaaaagact acgcggtcgc cgcaccacga
     5341 ggccatgtgc aacgcgtcaa ccatgggctg gaaaccgtgc atggacgcaa aacctacaag
     5401 atggaaacca tcctgcctcg cgacccgcgg cttggttatt actgctaccg aatgatgcat
     5461 tggacggatt tcgtgcgcgg gatcgaggtc aaatccgagg tgtataactt ccaggacgag
     5521 ctgcaggaag cctacgatta caaggatgtg aatgacgcgc cggggttttc tgatctggac
     5581 ttcgatcccc acaacaaggc gtacaaattg tgatcagccc tctgcatcca tggcttgtgt
     5641 gaaccagcac gcttcaaggc gtcgccgcat ccgaggccgg gctttgcgat gaggtgggcg
     5701 ctgcccccgc aacatccttg caacaccgaa aaccaacttc atatcccgtg tatttttcat
     5761 cgtgggaggt cacgatcgga cgacatcgcg agcgtaccgg cccccaccaa ccgcctttga
     5821 tggccccacg aaaaggtgct ttcccccacg ggacgaaaac ccactcgttc acattgccgt
     5881 tcaaatcgta aatgccgaaa ggagaaacgc acgtggtgcg ctcacctatg gcatggcgtt
     5941 gatcgagctt ggcaaactcc gccgcgcatg ccttgttctt catgcattcc ccatatggca
     6001 aaagctcacg gttcttcttt cgccactcct tgtcgatatt gcacttcgat gggtcacgct
     6061 cgaaccccgt ggcatacgga agcatgtctt ccccctc
//
LOCUS       DAOSOR010000346         6094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13047_length_6094_cov_4.516145, whole genome shotgun sequence.
ACCESSION   DAOSOR010000346 DAOSOR010000000
VERSION     DAOSOR010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13047_length_6094_cov_4.516145"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PK710_16730"
     CDS             <1..393
                     /locus_tag="PK710_16730"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOT11430.1"
                     /translation="CSILVDLRLVPGQTMESVQGQMLEITRQVASDFEVEIVDSRAPH
                     AMSPSHPLIDAIQRNAEPVLGFRPEPVGLGGATFAKWLNLAGIPSVGWGPGDDNAFHV
                     ADEYVSIEELVQFSLLIGLVALDLLGDD"
     gene            506..886
                     /locus_tag="PK710_16735"
     CDS             506..886
                     /locus_tag="PK710_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11431.1"
                     /translation="MRNLLLCVALVFPLCLNGCSKDPKERLQGKWGGQTVKQIDESQK
                     AEATTWAQGVRFEFDKNKMTVSLPSDQPRSGDFDIEDVSGDKLTVRVARESGGSDTAA
                     LRFEGSKLVWDVGSGREVVMTRVD"
     gene            1023..1505
                     /locus_tag="PK710_16740"
     CDS             1023..1505
                     /locus_tag="PK710_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11432.1"
                     /translation="MATPSKPKPSNQPDDEYQLPIQKTNPLIFVGVGAAVVLLAVVGF
                     LLTRGKQESKAEGDIEAAVHSARITADEAKAKREAQLEHLELAAKAWAIASEQERAAA
                     AASAAAAAHAEEKPPTVAAGEPKDPSGAAPPPKKKASSNDLDQLDKLGSAVNSELGGP
                     "
     gene            complement(1826..3298)
                     /locus_tag="PK710_16745"
     CDS             complement(1826..3298)
                     /locus_tag="PK710_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11433.1"
                     /translation="MQHECEIGSRFRPGSNRVGVRADRQGSSLSTNVRLSLETVITAA
                     VRRQAPISVETAGYIALAVADALVSASAVVRPCHVFITLEGEILLKGAQRGDDGTAET
                     SVRSLLASLLSVAVGTSPALTASSRRTGRRGLTLLVEELEAALIPVNRAAARRAIGRL
                     AREVQRSGIQSTPRLTHGRPSQRSARDVSKESRVSPACAVDDPGKITVQVDDPLSEIT
                     EVDAPVGPVAPAFTELSTAEPTAPNDSAASEEESTPAAPPPLNEAQYSVDTLFCGSTT
                     ELSEPPELMAPSVSAAFDEALSPGVDELPDERPEILEASSNEAWELEGSYDDLQTYEP
                     PEAVGDESLDFGTASPPQVEPIALEEPSIEEISDLEVPAPQAPCASSGLCTSGSDRVH
                     ELLEEFRTPERASREWAGELKEMLGLPPTPPPPVACQEAGESAADSEPSTEQEPPPPL
                     TFRPPRHPRLGIALTMVLLVLAIAVLLTCYVLHPALLMGG"
     gene            3414..>3934
                     /locus_tag="PK710_16750"
     CDS             3414..>3934
                     /locus_tag="PK710_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT11434.1"
                     /translation="MRTCPRCATPLAEQAVLCPACGIPIHQVVGPAPTPDHQTRVPVV
                     PAAIASSTNKQTMIGMPETMDPALLARLRASASEASLAAAPTVQPQSTAPQAPAKTML
                     GMAMPGVAPTQQPAAAAPTGPIHVPAQNKTMLGVAMPGVAMPGVAMPGVAMPGFAPTQ
                     QPAAAAPTGPIHVP"
     assembly_gap    3935..4034
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4035..5664
                     /locus_tag="PK710_16755"
     CDS             <4035..5664
                     /locus_tag="PK710_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11435.1"
                     /translation="AQNKTMLGVAMPGFAPTQQPAAAAPTAPAPMPAKKKTMLGVAMP
                     GIAPTQQPAAAAPTAPAPMPAQKKTMLGVAMPGIAPTQQPAAAAPAGPAQVPSQKKTM
                     LGVAMPGIAPTDGRVVPMSPPHVFTEAPIPPPAFGMDQSRAARPMVPWYRRLSFVFVA
                     LGVSVLLVVGLFAWLWPTKPPIASQALVNEQGRDVLRLTCASCPDGTVMTVEGASATV
                     AANVADVLLPKPLSVGDNRFQVRIDRPGSGRDEDVELRMPVSFRVRPDLTGLTADPPT
                     IHVEVETLPGTTVLVDGVAIHPDTSGKARYPVDVTEACTGPNTDSMLIDRTISYTIQP
                     KGAAASSGTVTVKVRVTPLTLQAPRPHTVVDTESFLVAGRTPRGATVQIEGARFEADK
                     DGAFSRTLQIKQLGETTVRVRAILPEHAPRTISFSVKRVERLEEEANTFEKNSQVEAR
                     RVMEEAAAYRGKQVVLRGEVIEARSQSSIRVILLDATGTCSAPPCLARLLYAGTEPLS
                     KGDFIRVFGRVADVQSAGGKTVPEIDVDFLLKGR"
     gene            5784..>6094
                     /locus_tag="PK710_16760"
     CDS             5784..>6094
                     /locus_tag="PK710_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11436.1"
                     /translation="MAEMLAKQQGKQMTRGLAMACAFVATIGAVSSFAEADDTKVNLP
                     IHLIVGPPAGPHPMARVNGSRTGLVAQLPSSPKLLWHQRVRGGTTLPVAVDETGAMVI
                     A"
BASE COUNT         1283 a         1788 c         1747 g         1176 t
ORIGIN      
        1 tgctccatcc tcgtcgatct tcgcctggtt cccggtcaaa cgatggaatc ggttcagggg
       61 caaatgctcg aaatcacacg gcaagtggcg tcggacttcg aagtggagat cgtcgatagt
      121 cgggcgcccc acgcgatgtc gccttcccat cccttgattg atgccatcca gcgaaatgcc
      181 gaacccgtgc ttggatttcg gcccgaaccg gtgggattgg gaggagctac cttcgcgaaa
      241 tggctcaacc tcgcggggat tccgtccgtg ggctgggggc cgggcgatga caacgctttc
      301 cacgtggccg acgaatacgt ctccatcgaa gaattggttc agttttcctt gctcatcggg
      361 ctcgtcgccc tcgacttgct cggcgacgac tgaagcgtcg gcccacccat ggcccattgg
      421 ccgctttgcc tcgacgaacg gcgcacccca tatacttggc caccttccgc caacgccgta
      481 ggttctcgtt cgtcggagga ccatcatgcg taatcttctc ctgtgtgtcg cccttgtttt
      541 tcccttatgc ctcaacggat gttccaaaga tccgaaagag cggctgcaag gcaaatgggg
      601 cgggcagacg gtcaagcaaa tcgacgagtc tcaaaaggcg gaggcgacga cttgggcgca
      661 gggcgttcgt ttcgaattcg ataaaaacaa gatgaccgtg agcctgccct cggatcagcc
      721 acgttctggc gatttcgata tcgaagatgt ttccggcgat aagttgacgg tccgtgtggc
      781 gcgggaatcg gggggcagcg acacggccgc cttgcgattc gaaggctcca aactcgtttg
      841 ggacgtggga agcgggagag aagtggtgat gacgcgggtg gactaagccc actggaccgg
      901 cacgaaagcc aggggtattg tatccgggtg ctcggtcttt ttcgcgcatg aacccatgcc
      961 gttcgatcgg gcgcttgcta tcgtgaagac aacctgagtt ttcagccaat gacggggtga
     1021 gcatggcaac gccttccaaa cccaaaccat ccaaccagcc cgacgatgag tatcaacttc
     1081 cgatccagaa aacgaatcct ttgatcttcg tgggagttgg ggccgcggtt gtcctattgg
     1141 cagtcgttgg attccttttg acgcggggca aacaagaatc caaagccgaa ggtgatatcg
     1201 aagcggccgt gcattccgcg cgcatcacgg ccgacgaagc gaaagcaaaa cgggaggccc
     1261 aactggaaca tctcgagttg gctgccaaag catgggcgat tgcctcggag caagaacgtg
     1321 cggcggcagc agcctctgca gcagccgctg cccatgcgga ggaaaaacct ccgaccgtgg
     1381 cggccggcga gcccaaagac ccctcgggcg ctgcccctcc ccccaagaaa aaagctagca
     1441 gtaacgacct cgaccaactc gacaaactcg gaagtgcggt caattccgaa cttggtggac
     1501 cgtaaccgta acgttacgat cgccctcctg cccatgattc cccgtgtttt tttcgcatcc
     1561 aagtcggatg gcgtcacatc tcgagcccta aatgaataaa ttatccatat tttccgttat
     1621 attgtccgtg aatctataga ataaatataa aatatttatg gcctgaaggt tgctattgcc
     1681 taggtcactc aacctccaag acagtgcttt tcttccttat cttccttatt atcttaattt
     1741 attcctgacc gcgacacctt atccccaccc caagcgtctc ctaaaatagc ttataattca
     1801 ataacttaag cctggagccc cgaatctagc cccccatcaa caacgccggg tgtaacacgt
     1861 aacacgtcag caatacggca atggcgagca ccaacaagac catcgtcagc gcaattccga
     1921 gacgaggatg gcgcggaggc ctaaaagtta ggggcggagg aggttcttgc tcggtcgatg
     1981 gctctgaatc cgcagccgac tcccccgctt cttgacaagc cacgggcggc ggcggagttg
     2041 gcggcagccc tagcatttcc ttcaactcgc ctgcccactc ccgactcgct cgttccggcg
     2101 tcctgaattc ctcgagcaat tcatgaaccc ggtcagaacc ggacgtgcaa agaccactcg
     2161 acgcacaagg cgcctggggc gcgggcacct ccagatccga aatctcctcg atactcggtt
     2221 cttcaagggc aatgggctcg acctgtggcg gagaagccgt cccaaaatcc aatgactcat
     2281 cgcccacggc ttccggaggt tcgtaggtct gcaaatcgtc gtacgaaccc tccaactccc
     2341 aagcctcatt cgatgaggct tcgagtattt caggcctttc gtcgggcaat tcatcgacgc
     2401 ctggagacaa cgcttcatcg aatgccgcgg aaacgctcgg ggccatcagc tccggcggtt
     2461 cggacagctc cgtcgtggac ccgcaaaaca acgtatcgac gctgtattgc gcctcgttta
     2521 ggggtggcgg ggctgctgga gtcgactctt cctcggatgc cgcggaatcg ttcggcgctg
     2581 tcggttccgc ggtggatagt tcggtaaaag cgggggcaac cggaccaacg ggagcgtcga
     2641 cctcggtgat ttccgacagc ggatcatcaa cctgtaccgt gattttcccg ggatcatcga
     2701 cagcgcaagc gggcgaaaca cgggattcct tcgatacgtc tcgtgcggac cgctggctcg
     2761 gtcggccatg ggtcaagcgc ggagtgcttt ggataccgga gcgttgaacc tctcgggcca
     2821 atcttccaat ggcgcgccgt gcggcagccc gattgactgg aatcaatgct gcctcgagtt
     2881 cctcgaccaa gagcgtcagt ccgcgacgac ccgttcgacg gcttgacgcg gtcaatgccg
     2941 gggacgtgcc aaccgccacg gaaagcaggg acgccaacag cgatcgcaca ctggtttccg
     3001 cggtgccgtc gtcaccccgt tgggctcctt tcagcaggat ttcgccctct agagtgatga
     3061 aaacatgaca aggccggaca acagcggatg ccgaaaccag ggcgtcagcc acggccagag
     3121 ctatatatcc ggcggtttcg acggaaatgg gtgcttgtcg tcgaaccgcc gcggtgatga
     3181 ccgtttcgag cgacaggcgg acattggtgg atagggaaga gccctgtcgg tcggcgcgaa
     3241 cgccaactcg attcgaacca ggacgaaaac gggagcctat ttcgcactca tgttgcatgg
     3301 ctatttattc ccataccgtc atgcgagcct tttgagcaac tttcagcagg tttcaacagg
     3361 gacgggttca acacgaattc agcgaacggc tgacaacctg atacaatggc cccatgcgaa
     3421 cgtgtcctag gtgtgcgact ccgttggccg agcaggcggt actatgcccg gcctgtggca
     3481 taccgattca ccaagtcgtt gggcctgctc ccacaccgga ccaccagaca agggtgcctg
     3541 tcgttccggc cgcgattgcc tcatcgacga ataagcaaac catgatcggc atgccggaga
     3601 cgatggatcc ggcgttactg gcaaggttgc gggcaagcgc gtcggaggca tcactcgctg
     3661 ccgctcccac agttcaaccc caatctaccg caccgcaagc gcccgccaaa accatgctag
     3721 gtatggccat gcccggcgtt gcgcctaccc agcagccagc ggcagcggcc cccacgggac
     3781 ctatccacgt gccagcgcaa aacaaaacca tgctgggcgt cgccatgccg ggcgtcgcca
     3841 tgccgggcgt cgccatgccg ggcgttgcca tgcccggctt tgcgcctacc cagcagccag
     3901 cggcagcggc ccccacggga cctatccacg tgccnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnagcgca aaacaaaacc atgctgggcg tcgccatgcc gggcttcgcg
     4081 cctacccagc agccagcggc agcggccccc acggcgccgg cccccatgcc agcaaagaaa
     4141 aaaacgatgc tcggggtggc catgccgggc attgcgccta cccagcagcc agcggcagcg
     4201 gcccccacgg caccggcccc catgccagca cagaaaaaaa cgatgctcgg ggtggccatg
     4261 ccgggcattg cgcctaccca gcagccagcg gcggcagccc ccgctggacc agctcaagtg
     4321 ccgtcgcaga aaaaaacgat gcttggggtg gccatgccgg gcattgcgcc aacggatgga
     4381 cgcgtggttc cgatgtctcc gccgcacgta tttacggaag ctccgattcc tccgccggcg
     4441 ttcggcatgg atcaaagccg cgcggctcgc cccatggtcc catggtatcg gcgtctttcg
     4501 tttgtattcg tagcgttagg tgtttccgta ttgttggtgg tgggtctgtt tgcgtggtta
     4561 tggcccacca agccgcccat tgcctcacaa gcactggtca acgagcaagg gcgagacgta
     4621 ttgcgtctga cctgcgctag ctgtccggat ggcacggtaa tgacggtgga gggggcatcg
     4681 gcgacggtag ccgcgaacgt ggcggatgtg ttgttgccaa agcctctttc cgtgggagac
     4741 aaccgttttc aggttcggat cgaccgaccg ggttccggaa gagacgaaga tgtcgaactg
     4801 cgcatgccgg tctcttttcg cgtacgacct gatttgacgg ggctcacggc agacccaccc
     4861 acgattcacg tggaagtcga aacgctccct ggcacgacgg ttctggtgga tggtgttgcg
     4921 attcacccgg ataccagcgg taaagcccgt tatcccgtgg atgtgacgga ggcatgcaca
     4981 ggaccaaaca ccgatagtat gcttatcgac cgcacgatat catacaccat tcaaccgaaa
     5041 ggagcagcgg cgagcagtgg gacagtgacc gtcaaagtaa gggttacgcc cctcacgttg
     5101 caagcgcctc gcccccatac ggtcgtggat acggaaagct ttctggtggc gggacgcacg
     5161 ccacggggag ccacggtgca gatcgaggga gcacgcttcg aggccgacaa ggatggcgct
     5221 ttttctcgaa ccctacagat caaacaactt ggagaaacca cggtacgcgt tcgtgcgata
     5281 cttccggagc atgcacctcg aacgatctcg tttagcgtca agcgtgtcga acgtctggag
     5341 gaggaagcca atactttcga aaaaaactcg caggtcgagg ctcgtagggt gatggaagag
     5401 gcagcggcgt atcgtggcaa acaggtcgtg cttcgaggtg aggtcatcga agcgcgttca
     5461 caaagctcca ttcgtgtgat cttactcgac gccacgggca cgtgctccgc acctccttgc
     5521 cttgctcgtc tcttatatgc cgggacggag cctctatcga aaggcgactt catccgcgtg
     5581 tttggtcgcg tcgccgatgt gcaatccgca ggtggaaaaa cagtcccgga aatcgatgtc
     5641 gacttcctgt tgaaaggtcg ttgacggcaa agacgcatcc tgagctgaac gtgaacaacg
     5701 acgagaaggt ttcgaaaccg gcgcatgttc aatccccagg aacgacgacg tgggggagaa
     5761 acggtttttg gacaatggtg aggatggctg aaatgctggc aaagcagcag ggaaaacaaa
     5821 tgacccgtgg actcgccatg gcttgtgcat tcgtcgccac gataggtgcg gtcagctcgt
     5881 ttgccgaggc cgacgacacg aaagtgaatc tgcccattca tcttatcgta ggaccgcctg
     5941 ctggcccgca ccccatggca cgggtgaacg gttcgcgtac gggcttggtg gctcagcttc
     6001 cttcgtctcc caagctgctg tggcatcagc gtgttcgagg aggcaccaca ctaccggtcg
     6061 cggtcgatga aaccggtgcg atggtgatcg ccag
//
LOCUS       DAOSOR010000347         6090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13064_length_6090_cov_4.429660, whole genome shotgun sequence.
ACCESSION   DAOSOR010000347 DAOSOR010000000
VERSION     DAOSOR010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13064_length_6090_cov_4.429660"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..1299
                     /locus_tag="PK710_16765"
     CDS             235..1299
                     /locus_tag="PK710_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11437.1"
                     /translation="MMKTLVLNSSQPKYPLGSDPWVKATLHAVDEAAALGHTIVASVG
                     LACWELALWRSAQRNARIHVILPLLGKQQPNTAAEAWSRSFGLPYDRTTFTWIPTNNP
                     KAWWPLRDRIAIRRSDNIVPICLRPSGRLAKELQEAQAEVSPHHAIPWSPKTPTTGWQ
                     NRFPLLIQELWPYGALVHLTRACSGPWPGESHESYYHDVVASTQTYARSGLATLIRII
                     REGRIRGSLFRARANRARVCFSACTTREALGLVRFRSRFARYAFEPYAIVLLAGAIQR
                     LKAHPVRYDANESTDLFGQHPGIHGVWSAEKEWRVLGDVDLRSLDPGDVRVVTATDKE
                     AESLRPWCPWEVRSFGCFSA"
     gene            1357..2019
                     /locus_tag="PK710_16770"
     CDS             1357..2019
                     /locus_tag="PK710_16770"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT11438.1"
                     /translation="MSLRKKVANLLAQGQLDEIAALTDTTPTVASAVTSRLYDADALA
                     RFRAADALGRVAARIITSHRVSVEQLLHKLAWALNEESGATAWGAPHAMGEIARIDPT
                     LASQYQPLLLSYLDDEEVYLGTDIMVHGVVWALGRVGERFPELGAAAVPALLKRLQDP
                     DDSTRALSVEALGRIGDGRAKEPLLAQTGDETRVERYLDGQMVPVSIKTLAVAALERL
                     RP"
     gene            complement(2032..2223)
                     /locus_tag="PK710_16775"
     CDS             complement(2032..2223)
                     /locus_tag="PK710_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11439.1"
                     /translation="MAGRLHAVVAQEEHAVAEAKQQLEELGDEQVDLQKLAKVAIDFL
                     KDMQSAKEQVDRATAFHRA"
     gene            complement(2656..3861)
                     /locus_tag="PK710_16780"
     CDS             complement(2656..3861)
                     /locus_tag="PK710_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11440.1"
                     /translation="MTSVGAVGCPAYGPVMGDNAVGTPAQGAIPGLPSPLLDAATPDD
                     VLGMMMAAVERMASLSVDENQEQVRVHQQKLERAIDDFLDKIAASIEAARKAAAERAK
                     EKSRGLFGTIASSVGKVCANVVGSVIDFAKDAVEAPFEIGIALVKNGGNPLQAFQSAL
                     VDQFQQLTTDGDVAASVAGFTEGVLEFTADFADFCVACSMDFATGRLDRLDDAAGKLW
                     ESLSDHILMNPAFWDVVEPLAKAVTLAGAVASGGALAPIAVGLMLFLEVDSKTGFLER
                     AVGEQAAPYLRMGLALAASSLTLGASGQTATLARYVLGGTTALEGLATVEQGRLDLEQ
                     ARRIREKSLENIGLQDAQHRVQMLHQLVDELVEALGEAMDTRKTNQMMFQSLAQTQMQ
                     VSSAVVFRA"
     gene            complement(3858..4247)
                     /locus_tag="PK710_16785"
     CDS             complement(3858..4247)
                     /locus_tag="PK710_16785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11441.1"
                     /translation="MMCSDNHGNTLESLVEPCYSAAYRAWQDEDGSTAKRLFGCLAAM
                     APKDERGWMGLATVYERERKWHVAVGFYGVATVMAPGNPWCHLGKARCLMRMGREPQA
                     RRTFDLAESLSDDADFLDALERERGER"
     gene            complement(4254..4799)
                     /locus_tag="PK710_16790"
     CDS             complement(4254..4799)
                     /locus_tag="PK710_16790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11442.1"
                     /translation="MDLDDTNGLSAEGLLLYLQTRLNGLDEQINGIFKKMQDMEKIRK
                     LLNQMQNELNQLCDDRTKSGCCGTSHEAGKPEGYEKNLIDCIDAIGQIDPGLAQTLRN
                     QLGVEGQILYGLDGRYLTNEVNNSRELLNSVAKQLESGAQLDMIRLQSLTATRGTAIQ
                     MATNTLATLNDSLKGVVHNIK"
     gene            complement(4998..5213)
                     /locus_tag="PK710_16795"
     CDS             complement(4998..5213)
                     /locus_tag="PK710_16795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11443.1"
                     /translation="MDTHVVQGSLPSFEQEIRRQDVMWEALREAMSETDASVRFLLSE
                     EWLSGWEQMGAQHAQPMGENTGFSYRA"
     gene            complement(5869..>6090)
                     /locus_tag="PK710_16800"
     CDS             complement(5869..>6090)
                     /locus_tag="PK710_16800"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOT11444.1"
                     /translation="PVDSVVKGLHIALSDRIVSMAGRLLMGVETVLLSGGVASNSAMV
                     AALSSKVGKPVAVVPEPQFVGALGAALLG"
BASE COUNT         1307 a         2015 c         1469 g         1299 t
ORIGIN      
        1 gtgaatccga tgtctcccca cccccactgc aagcaggaat caagaaggca agcataagcc
       61 ccgcatgaca cgccaccttg ctcacacgac caaccttcat cgttcgatcc tttcccttgc
      121 cacgcatcac taagccatca tcaatgactc tctttatcag attccaagac gaacgcacgc
      181 ggatgcgaat gcacaaatca tcgcacaacc aaacccatcg atgaccgacc atgaatgatg
      241 aaaacgctcg ttctgaattc gagccagccc aaatatccct tgggctccga cccttgggta
      301 aaagcaaccc tgcatgccgt cgatgaggcc gccgcgctag gacacaccat cgtggcttcc
      361 gtgggattgg cctgctggga actcgcgttg tggcgaagcg cccaacgcaa cgcccgtatt
      421 cacgtgatcc ttccccttct cggaaaacag cagcccaata cagccgccga ggcttggtct
      481 cgcagcttcg gtctacctta cgatcggacc acctttacct ggatccccac gaataacccc
      541 aaagcatggt ggccgctgcg cgaccgtatc gccatacgac gtagcgacaa tatcgttccc
      601 atctgccttc gcccctcggg aaggctcgcc aaagagctgc aagaagccca agccgaagta
      661 tctccccacc acgcgattcc ctggtccccc aaaaccccta ccacggggtg gcaaaataga
      721 tttcctttgt taattcaaga actttggccg tatggcgccc tcgttcatct cacccgtgcc
      781 tgctctggcc catggcccgg ggaatcccat gagagctatt accacgacgt cgtggcaagc
      841 acacaaacct acgcgcgttc aggattggct accttgatcc gcatcatccg ggaaggacga
      901 attcggggct cgctgtttcg ggctcgtgcc aaccgagccc gcgtgtgttt cagcgcctgc
      961 accacccgtg aggcacttgg actcgtgcga tttcgctcgc gattcgcccg ctacgccttc
     1021 gagccctacg ccatcgtatt gcttgctgga gcgatacaac gactaaaggc tcaccccgtt
     1081 cgctacgatg ccaacgaatc gaccgatcta ttcggacagc atccgggaat ccacggcgta
     1141 tggtccgcgg aaaaagaatg gcgggtgctc ggcgatgtgg acctgcgcag cctggatccc
     1201 ggcgacgtgc gcgtggtgac agcaacggat aaggaagctg agagccttcg cccctggtgt
     1261 ccctgggaag tgcgtagttt tggctgtttt tcggcgtgag cgaataaaac gatacaaccg
     1321 cgattccttc gggagtctcg atgcatccaa aggaaggtga gcctacgaaa aaaagtcgcc
     1381 aacctgctgg cccaaggtca attggacgag attgccgccc tgaccgatac cactcccacg
     1441 gtggcgagtg ccgtgacgag tcggctttat gatgcagatg ccctcgcacg attccgcgcc
     1501 gctgatgccc tcggccgcgt ggcggcccgt atcatcacct cccatcgtgt ttcggtcgaa
     1561 caactcctgc acaaactggc ctgggcgctg aacgaagaat cgggagccac tgcctggggc
     1621 gccccccatg ccatgggcga aatcgcccgt atcgacccta ccttggcctc tcaatatcag
     1681 cccttgctgc tttcttacct cgacgatgaa gaggtttacc tgggaaccga tatcatggtg
     1741 catggcgtcg tatgggcgct aggacgtgtg ggagaacgct tcccggaact tggggcagca
     1801 gcggtcccgg cgttgttgaa aagactccaa gacccggatg actcgacccg agccttgtca
     1861 gtcgaagcct tgggacggat tggtgatggc cgcgccaaag aaccgctgct agcccagact
     1921 ggcgatgaaa cccgggtgga gcgctacctc gacggccaga tggtgcccgt aagcataaaa
     1981 acccttgcag tggcagcctt ggagcgattg cggccatgat ccggtccacg atcacgcgcg
     2041 gtgaaacgca gtggcacggt cgacctgttc tttggccgat tgcatatctt tgagaaaatc
     2101 aatcgcaact ttcgccagtt tctgcaaatc cacctgctcg tcccccaact cctcgagttg
     2161 ctgcttcgct tccgccaccg catgctcctc ctgcgccacc accgcatgga gtctgccggc
     2221 catgcgcgaa tccccagccg ccaaaaagtc catcgatgcc gacgatgcct tgccccggac
     2281 agcacttgac tggtgcccac tccgttttcc cctttcgtca tgacgcaccc actggcgacg
     2341 gttagtcccc cgtccagtaa cttcccaaac gatccggccc gagccgcttg atattcccga
     2401 ttcgccttgt cccgtaatgc ctgcatctgg ctttcgtgat gttgttggat tcgttcccgc
     2461 tgacattgac gcatcatgcg tacgtccgtt cccttatccc tggcttgcac caacaacagg
     2521 gcggcgactt gggcatgcgc atcacctccc agttccgccg caagaacgtc ctgcgacgcc
     2581 aacgccattc ggctcacgca accgctatcg actgtcatcg acatcactcc tcctcaccct
     2641 tgcctggaat cgtcttcagg ctcggaaaac aactgcagaa gacacttgca tttgagtctg
     2701 tgccaacgat tgaaacatca tctgattcgt ctttcgcgtg tccatcgcct ccccaagcgc
     2761 ctccaccagc tcatcgacaa gttgatgaag catctgcacg cgatgctgcg catcttgtaa
     2821 ccctatgttt tctaatgatt tttctcgaat ccgacgcgcc tgctccaggt ccaatcgtcc
     2881 ctgttcgacc gtcgccaatc cctccaaggc cgttgttcca ccaagaacat accgagcgag
     2941 cgttgccgtt tgccccgacg ctcccaaggt caatgaggag gccgccaggg caagacccat
     3001 ccgcagatac ggcgcggctt gctcacccac agcgcgttcg agaaacccgg ttttcgaatc
     3061 gacctcgaga aaaagcatga gtcctaccgc aatcggcgcc agggcacctc ccgaagccac
     3121 ggcaccggcg agggtaacag ccttggcgag cggctccacc acatcccaga aggccggatt
     3181 catgaggata tgatccgaca agctctccca tagctttccc gctgcatcgt cgagacgatc
     3241 caaacgaccc gtggcgaaat ccatcgagca agccacgcaa aagtccgcga agtccgcggt
     3301 aaactccaag accccttccg tgaaacccgc gaccgaggca gccacatctc cgtcggtggt
     3361 cagttgttga aactggtcca ccagagcaga ctgaaaggct tgcaggggat tgcctccgtt
     3421 tttgaccagc gcgataccaa tttcgaaagg ggcttccacc gcatccttcg caaagtcgat
     3481 gaccgacccg accacgttgg cgcatacttt gccgacggaa ctcgcgatgg tgccgaaaag
     3541 accacggctc ttctcctttg cgcgctcggc ggccgctttt cgagccgctt caatcgatgc
     3601 tgcgattttg tcgagaaaat cgtcgatggc ccgctccaac ttctgctgat ggacgcgtac
     3661 ctgttcctgg ttttcatcga ccgacaagga agccatcctt tcgaccgccg ccatcatcat
     3721 cccaagaacg tcatccggcg tcgccgcatc caacagcgga gaaggaagtc cgggaatcgc
     3781 tccttgtgcg ggtgtgccca ccgcattgtc acccatgaca ggcccataag cagggcaacc
     3841 cacggctcct accgacgtca tcgctccccc ctttcccgct ccaaagcgtc gagaaaatct
     3901 gcatcatcgg acaaagactc cgccagatcg aacgttcgcc gcgcttgcgg ttcccttccc
     3961 atccgcatca aacaccttgc tttccccaaa tgacaccagg gattgcccgg cgccatcacc
     4021 gtcgctacac cgtagaaccc caccgcaaca tgccattttc gctcccgctc gtagaccgtg
     4081 gcaagcccca tccaacctcg ttcgtccttg ggtgccatgg ccgccaggca cccaaataac
     4141 cgtttggccg tagagccatc ttcgtcttgc catgcgcgat aggcagcaga ataacagggc
     4201 tcgactaagg attcgagcgt atttccgtgg ttatcagaac acatcatcgc aacctacttg
     4261 atattatgta caacaccctt gagcgagtca ttgagcgtcg ccagcgtatt ggtcgccatc
     4321 tgaatggccg ttcccctcgt ggccgtcagc gactgcaacc gaatcatatc gagctgtgcc
     4381 cccgattcga gctgcttggc cacactattg agcaactccc gcgaattgtt gacttcgttg
     4441 gtcaaatatc gaccatcgag cccataaaga atctgcccct cgacaccgag ctgattcctt
     4501 aacgtctgcg ccaaccctgg atcaatctgc cctatcgcat cgatacagtc gatcaggttc
     4561 ttttcatacc cctcgggttt acccgcctca tgggaagttc cacaacagcc cgacttggtg
     4621 cggtcatcac aaagctgatt gagctcgttt tgcatctggt tgagtaattt tcttattttt
     4681 tccatatctt gcatcttttt gaagatgccg ttgatctgct cgtcgagacc gttcaaccgc
     4741 gtttgcaaat acagaagcag cccttccgca ctcaaaccgt tcgtatcgtc cagatccatg
     4801 accgaaacgt cctgactcgc agatacccgg cccgccggat ccgtatactc cacatcagcc
     4861 gccgaaaatc cttgattcat ccctatcgga agcggaagac gctggtggaa catcggcgct
     4921 tgactactat ccatcatcgg ctcatctccc ctttctcgtc gactcttgcc atcgacgctt
     4981 caatcctggc cgctccttta cgcgcgatac gaaaaacccg tgttttcacc catgggctgc
     5041 gcgtgctgcg cacccatctg ctcccagccg gacagccatt cttccgaaag cagaaaccgc
     5101 acagaagcat ccgtttccga cattgcctcc cgcaacgcct cccacatcac atcctgccgc
     5161 cgaatctctt gctcgaacga tggcaacgaa ccttgcacga catgcgtatc catgagttta
     5221 tctccttgtt tatcgatgcc cctgcaccgg ctttcctgcc tattcggccc ccgccttccc
     5281 aggttgcgaa aaaattttgc cagacagatt ctttttttgg aaatgggcac gcgcagcgac
     5341 ggacttcttc gatcgcctgc cttcgagcct ccagcacaga attggccacc cgagtccaac
     5401 tacgccttat atgtatccac ccgccatttt caccgtttcc aacattatca gaaggtggac
     5461 aggttttgtt ggcgtgacca tcccaccgta gccacgagat gcggcttggg gactcgacca
     5521 gcctgagagg gctattgcct gaataatccc cttatttccc ctgaattgga aagggacacg
     5581 ggctgaggat tcttgggtct tgattttggc tcggtccttg actcccccct tggagcaccc
     5641 ccaacagttg ccctgagcta ctcaacagtg gactggcgtt ctgcggtgaa caataggaga
     5701 tgaccgtggc cgtttttgtg tgccgtgcgc cgtgaccgtg accgtgaccg tagccgtggc
     5761 cgtaaccgtg aaggaaaggc cagcaaataa tgaggggatg gctcaactct tccccatcga
     5821 atcctacagg actccccaac atcacccttg cacgtcggcg tgcctccctt atcccaacaa
     5881 cgccgccccc aacgccccca cgaattgcgg ttccggcacc accgccaccg gcttgccaac
     5941 cttggaagat agagcggcaa ccatggccga attgctcgcc accccaccgc tcaaaagcac
     6001 cgtctccacg cccatgagca atcgtccagc catcgatacg atgcgatcgg ataaggcaat
     6061 atgtaacccc ttgaccaccg aatccacggg
//
LOCUS       DAOSOR010000348         6090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13065_length_6090_cov_4.367688, whole genome shotgun sequence.
ACCESSION   DAOSOR010000348 DAOSOR010000000
VERSION     DAOSOR010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13065_length_6090_cov_4.367688"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="PK710_16805"
     CDS             complement(<1..1216)
                     /locus_tag="PK710_16805"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HOT11445.1"
                     /translation="MNRHSPQNRLADEHSLYLRQHASNPIHWRVWDDETLNDARSQDK
                     PIFLSIGYASCHWCHVMEEQVFEKHDVASFMNEHFISIKVDREERPDLDATYMQSVLR
                     MTGRGGWPLSVFLTPDLKPFFGGTYFPPDVFIELARRILDVFRNHREELNDQAERWTE
                     AIEQGFDKLVSHPFDARLLDDGAKEAEGSFDPHWGGFRASMKFPTPVRWRFILRRYRK
                     TGAQPLAQMVRKTLDAIAAGGIRDHLAGGFHRYSVDERWQVPHFEKMLYDNAQLASLY
                     LEAGAVFDHPPFFEITRETLDFLMREMQGKEGAFYASFDADSDGTEGAYYVWSLDELL
                     SIAGPRDGSLLASLFGVTENGTFEGANVLWRNTPIARVARDHGVDEKDVLDCFSRWRK
                     ALLSHRNQRKAPR"
     gene            1762..3984
                     /locus_tag="PK710_16810"
     CDS             1762..3984
                     /locus_tag="PK710_16810"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOT11446.1"
                     /translation="MSFVFYYLPIIVAFVALAYAFMRATWVTKQDPGNDRMKLIGKWI
                     ADGAMAFLAREYKSLAIFVVATAVILGVANHFTADDTNAMISVAFVFGAICSGLAGYL
                     GMRTATKANIRTASAARKGLNEALQVAFAGGSVMGLAVVGLALGGIAIMFVVFSTIYG
                     DSVNDDKAMMLVLNLVSGFSLGASSIALFARVGGGIYTKAADVGADLVGKVEAGIPED
                     HPLNPATIADNVGDNVGDVAGMGADLFESYVGAIVSAMILGVAWFGGGGLGIEAIAPG
                     TGMNAVYLPLVIAVIGIVASIIGFFFVRTKEGGNPQTGLNMGTFGAGALMVIGSVASI
                     YYLLPKEGIQVKDVLTGNTDTYQWWGVALAVVFGLLAGILVGVLTEYYTATFKKPVQT
                     IVDASRTGPATTVIQGIAVGMNSTTFPVLGICIAIIVAYEFAGLYGIALSAVGMLSTT
                     AIQLATDAYGPIADNAGGVAEMAQLGRDIRARTDKLDAVGNTTAAIGKGFAIASAALT
                     ALALTAAYLTKMNGEVKLNAADPYVLVGLLIGTMLPFRFSAMAMVAVGKAAQDMIQEV
                     RRQFREIPELRPALEAAQRAEHEEREPTEEEAKIIHAADDKAQYTKCVEISTAAAIKK
                     MVAPGLMAVLAPIAVGLYSANMLGGLLIGVTISGVCLAIFQANAGGAWDNSKKQVKEE
                     GHKKYGEAYDEMHKATVTGDTVGDPFKDTSGPSINILIKLISVVAMIIAPLLYSLHFQ
                     "
     gene            complement(4014..5417)
                     /gene="fumC"
                     /locus_tag="PK710_16815"
     CDS             complement(4014..5417)
                     /gene="fumC"
                     /locus_tag="PK710_16815"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOT11447.1"
                     /translation="MKTASETRTETDSMGSIEVPSTAYYGAQTARSLSNFRIGDERMP
                     REVIRALGIVKKAAAQANQQLGLLDANLASLIVRAADEVIEGKLDDHFPLLVWQTGSG
                     TQTNMNVNEVISNRAIEMAGGKLGSKSPIHPNDHVNQSQSSNDAFPTAMHVAIVEAIE
                     DRLLPRVRALRDTLDAKSQAFASIVKVGRTHLQDATPLTLGQEISGWVAQLDDADHAI
                     KSMIPALYRLALGGTAVGTGLNTHPGFADLVATKIGEITGRPFESAPNKFASLAGHDA
                     LVGASGAVKQLAVAAMKIANDVRWLASGPRCGIGELTIPENEPGSSIMPGKVNPTQSE
                     AMTMVCCQVMGNDATIGFAGSQGNFQLNVFKPVMVYNLLQSIRLLADACDSFRDHCAV
                     GIEPNRPVIERNVQRSLMLVTALNRRIGYDAAARIAKHAHHTGGTLKDAAIELKLLTA
                     EQFDEAVRPEKMVGPSE"
     gene            5661..>6090
                     /locus_tag="PK710_16820"
     CDS             5661..>6090
                     /locus_tag="PK710_16820"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOT11448.1"
                     /translation="MSEFKKVVIVGAVRTPIGSLGGGLASLQARELATHAIKALLDAT
                     KVDPSLVQYTCMGWVMQDPRSPNIAKTAAEFAGVPPTSPGTTFHENCASGGAAIHSLA
                     RRLMLGEVSLGVAGGVESMSNVPRYLFSGRFKGQVFGDLSL"
BASE COUNT         1281 a         1668 c         1749 g         1392 t
ORIGIN      
        1 gacgcggtgc ctttcgttgg tttcgatggg aaaggagagc cttgcgccaa cgagaaaagc
       61 aatccaagac gtctttctca tccacaccat gatctcgagc gactcgtgcg atgggagtat
      121 ttctccacag gacgtttgcg ccttcgaagg ttccgttttc ggtgactccg aagagagaag
      181 cgagaaggga cccatcccgt ggtcccgcga tgctgagcag ctcgtcgagg gaccagacat
      241 aataggcgcc ttcggtgcca tcgctgtcgg cgtcgaaact ggcataaaac gcgccttctt
      301 tcccttgcat ttcgcgcatg agaaaatcga gggtctctcg ggtgatctcg aaaaagggcg
      361 ggtgatcgaa cacggcacca gcttcgaggt acaagctggc gagttgcgcg ttgtcgtaga
      421 gcattttctc gaagtgcggc acctgccagc gttcgtcgac gctgtagcga tggaagccgc
      481 cggctaggtg atcgcggatg cctcccgcgg cgatggcatc gagggttttt cgaaccatct
      541 gtgccaaagg ttgagccccc gtttttcgat accggcgcag gatgaatcgc cagcgaaccg
      601 gtgtggggaa cttcatgctt gcgcgaaatc ctccccagtg gggatcgaag gagccctccg
      661 cttcctttgc cccgtcgtcg agcaatcgtg catcgaaggg atgagacaca agcttgtcaa
      721 acccttgttc gatggcttct gtccaccttt ctgcttggtc gttcaactcc tcgcgatgat
      781 tgcgaaaaac gtcgaggatg cgccgcgcca gctcgatgaa gacatcaggc ggaaaatatg
      841 tccctccaaa aaacggtttc agatcaggag tgagaaagac ggacaagggc catcctccgc
      901 gacccgtcat tcgtagaacg gattgcatat aggtggcgtc gagatccggt ctctcctccc
      961 gatcgacttt gatgctgatg aaatgctcgt tcatgaaaga agccacatcg tgcttctcga
     1021 acacctgctc ttccatcaca tgacaccaat gacatgacgc gtaaccaatg gataaaaata
     1081 ttggtttgtc ctgcgatcgt gcgtcgttca gggtctcgtc atcccacacg cgccagtgaa
     1141 tgggattgct ggcgtgttgt cgcaaataca gggaatgctc gtcagcgagg cgattttggg
     1201 gggagtgacg attcatggac tgtgttttcc aaaggtggtt ttggtgttgg atgaaagaag
     1261 ggacagaagg attgcgcgaa cggatggcaa gtattcgatg cggggaacgt ctggaccgat
     1321 ccggatggtc ttcgagcgaa caaaccgtga aatgcacgaa tagaagacta acgaggcatc
     1381 tagagtcgac ttggttgact gtccgaaaac ggatagcgtt cggactgggg tgtgaaaagg
     1441 tgggatgacg cgaggcgtaa tatgatgggg gcgtccagcg atagggaccg cgaggacgaa
     1501 aacgatcgga agatgatttt ccagaacacg aaccgcgaag caatctcgcg aataactaaa
     1561 ttgttgtgtg atattcaagg cttagcgtat cgtgacaaca atggggttag cataaccatt
     1621 gcgccttaga ggcgcctaaa tatgtcaaac tacttgaaat ttggcctttt cgttgacagc
     1681 cggaaagcac tctcctaacg gaacaacagt ccgacggtat tctcgactcc ctagcgagcc
     1741 cgtggaagga ggaggatcgg aatgagcttc gtgttttact atcttccgat aattgtggcg
     1801 ttcgtagccc ttgcctatgc gtttatgcgc gcgacctggg tcaccaagca agaccctggc
     1861 aatgatcgga tgaaactgat tggcaagtgg attgcggatg gcgcgatggc ctttctggct
     1921 cgggagtata agagcctggc gatcttcgtg gtcgcaaccg ctgtcattct cggtgtcgcc
     1981 aaccacttca ccgccgatga caccaacgcg atgatttctg tcgcgtttgt tttcggtgcc
     2041 atttgctctg gcctggcggg gtacctgggc atgaggactg ccaccaaggc caatattcga
     2101 acggcgagcg ccgcccgtaa gggactcaac gaagctttgc aagtcgcctt cgccggtggc
     2161 tccgtcatgg gcctcgcggt cgtcggtctc gccctcggcg gcattgccat catgttcgtc
     2221 gttttctcaa ctatctacgg tgattcggtc aacgacgaca aggccatgat gctcgtgttg
     2281 aatctcgtgt ccggcttctc cttgggagca tcgtccatcg cgcttttcgc tcgtgtgggc
     2341 ggtggtattt acaccaaggc agcggacgtc ggagcggact tggtaggcaa agtcgaagcc
     2401 ggtatcccgg aagaccaccc cctcaacccc gctactatcg ctgataacgt tggcgataac
     2461 gttggcgatg tcgccggtat gggcgccgac ctgttcgagt cctacgtggg tgccatcgtt
     2521 tccgccatga ttctcggcgt tgcttggttc ggcggaggcg gacttggtat cgaggctatc
     2581 gccccaggta ccggtatgaa tgcggtctat ctaccccttg tcattgccgt gatcggtatc
     2641 gtcgcttcca tcatcggctt tttcttcgtg cgcaccaagg aaggcggcaa tccccagacg
     2701 ggcctcaaca tgggaacctt cggagcaggg gctctgatgg tcattggatc tgtcgcgagc
     2761 atttattatc tgcttcccaa agagggaatt caggtgaaag acgttctcac cggcaatacc
     2821 gacacctacc agtggtgggg tgtcgccctc gccgttgtct tcggcctgct cgcagggatc
     2881 ttggtgggtg tgcttaccga atactacacc gcgaccttca aaaaacctgt ccaaaccatc
     2941 gtcgatgcct cccgcaccgg tcccgctacc accgtcatcc agggcatcgc cgtcggcatg
     3001 aactccacca cgttccccgt gctcggcatt tgtatcgcca ttatcgttgc ttacgagttc
     3061 gccggcttgt acggcattgc tctttccgcc gttggcatgt tgtccaccac cgccattcag
     3121 cttgctaccg acgcgtacgg tcccatcgcc gataacgcgg ggggtgtggc ggaaatggcg
     3181 cagcttggac gcgacatccg tgctcgcacg gacaaactcg acgccgtggg caacaccacc
     3241 gcggccatcg gaaaaggatt tgctatcgcc tccgccgctc tcacggccct tgcccttacg
     3301 gccgcctatc tcacgaagat gaacggcgaa gtcaaactca atgccgctga cccgtacgtg
     3361 ctcgtcggtc tgctcatcgg caccatgctc cccttccgct tttccgccat ggcgatggtg
     3421 gccgtcggaa aggccgcgca agacatgatt caagaggtgc gacgtcaatt ccgcgagatt
     3481 ccggaactac gtccagctct cgaggccgca cagcgtgcag agcacgaaga acgtgaaccc
     3541 accgaagaag aagccaagat cattcacgcc gccgatgaca aagcgcaata caccaagtgc
     3601 gtcgagattt cgaccgctgc tgccatcaag aagatggttg cccctgggct catggctgtt
     3661 ctcgccccca tcgccgtggg attgtattct gccaacatgc tgggcgggct cctgattgga
     3721 gtcacgatct cgggtgtgtg cctcgccatc ttccaggcca acgccggtgg tgcatgggac
     3781 aactccaaaa aacaggtcaa ggaagaagga cacaagaaat atggcgaggc ctatgatgaa
     3841 atgcataagg ccaccgtcac cggtgacacc gtaggtgacc ccttcaaaga tacctcgggc
     3901 ccctccatca acatcctcat caagctcatc agcgtggtgg cgatgatcat cgctccgctt
     3961 ctttactcgc ttcatttcca atgatcgaat tgctcgcttg actcacctgc ccttcattcg
     4021 ctcggtccca ccatcttttc cggtcgaacg gcttcatcga attgctcggc cgtcaataac
     4081 ttcaattcga tcgcggcatc cttcagcgtt ccgcccgtgt ggtgcgcatg tttcgcgatt
     4141 cgagccgccg catcgtagcc aatgcgacga ttcaacgcgg tgacgagcat gagcgagcgc
     4201 tgcacgttac gttcgatcac cggtcgattg ggctcgatac ccaccgcgca atgatcgcgg
     4261 aaactgtcac aggcgtcggc caacaaccgg atggattgca gcagattgta aaccatcacc
     4321 ggtttgaaca cattgagctg aaaattgccc tggcttcccg caaaaccgat cgtcgcatcg
     4381 ttgcccatca cctgacagca aaccatcgtc atggcttccg attgcgtcgg attgaccttg
     4441 cccggcatga tggaactgcc cggttcgttc tcgggaatcg tcaattcacc aatgccacat
     4501 cgaggtcccg aagcgagcca gcgaacgtcg ttggcgattt tcatcgcggc gacggccaat
     4561 tgtttgactg cgccgctggc ccctaccaag gcgtcatgac ccgcgagaga agcaaatttg
     4621 ttgggggcgc tttcgaaagg ccttccggta atctcaccga ttttcgtggc aacgagatcg
     4681 gcaaagcctg gatgcgtatt caagcccgtt ccgactgccg ttccgcccag agcaagtctg
     4741 taaagtgctg gaatcattga tttaattgcg tgatcggcat cgtcgagctg ggccacccaa
     4801 ccggatattt cttgccccag agtcaaaggc gtagcgtctt gcaggtgcgt gcgccctact
     4861 ttgacgatcg atgcaaacgc ttgactcttg gcgtccaaag tatctcgaag agcgcgcact
     4921 cgtggaagca aacggtcttc gatggcttcc acgatggcta catgcatggc ggttgggaac
     4981 gcatcgttgg atgattgcga ttggttgaca tgatcattgg gatgaatcgg tgacttggaa
     5041 ccaagtttcc ctccggccat ttcgatggct cgattggaga tcacctcgtt gacgttcata
     5101 ttcgtttgcg tgcccgatcc ggtttgccag accagcaggg gaaagtgatc gtcgagtttg
     5161 ccttcgatga cttcatcggc tgctcgaacg atgagcgaag cgaggttggc atcgagcagc
     5221 ccaagctgtt ggtttgcctg cgccgctgct tttttcacga tgccaagagc gcgaatgact
     5281 tctcgtggca tgcgctcgtc gccgatgcga aagttggaca gggagcgtgc ggtttgcgcg
     5341 ccataatagg cggtcgaggg cacctcgatg gatcccatgg aatcggtttc cgttcgtgtt
     5401 tcggatgcag ttttcatacg agcttcccgt ggtgcaaggt caatgatgga tcgggcgaaa
     5461 gcttggtgtt gatgatcgcg tggggcaagg gcacagaaca ggaaccatga cggagccttg
     5521 cttgcaggcg tttgattcgc tggcctggcg tggtgattgc acggaaacga tccaacggga
     5581 tcggaggctc cgaggctgtg gtacagtccc acgccgagga atcgaaaccg aatacgcgct
     5641 taaccgtggg agattaggtc atgagcgagt tcaaaaaagt tgtgatcgtt ggtgccgtac
     5701 gaacaccgat tggttctctt gggggtgggt tggcttcgct tcaagcgcga gagctggcca
     5761 cccacgccat caaagcactt ctcgacgcga ccaaggtgga cccatcgctc gtgcaataca
     5821 cgtgcatggg ctgggtgatg caagatccgc gatctcccaa tatcgcaaaa acggcagccg
     5881 agttcgcggg ggtgccaccg acgtcgccag gcacgacatt tcatgaaaat tgtgcatcgg
     5941 ggggagctgc gattcatagt cttgcgcgac gcttgatgct tggagaagta tcgcttggcg
     6001 tggcgggggg tgtggagtcg atgtccaatg tgcctcgtta tctgttttcg ggacgcttca
     6061 aagggcaggt gtttggcgat ctttccctgg
//
LOCUS       DAOSOR010000349         6076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13111_length_6076_cov_4.462548, whole genome shotgun sequence.
ACCESSION   DAOSOR010000349 DAOSOR010000000
VERSION     DAOSOR010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13111_length_6076_cov_4.462548"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            382..591
                     /gene="rpmI"
                     /locus_tag="PK710_16825"
     CDS             382..591
                     /gene="rpmI"
                     /locus_tag="PK710_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOT11449.1"
                     /translation="MAPKIKMRTNRSASKRFRLTGTGKIRRSKSGGNHGLSNKNRKRL
                     RRLRDNDTVASCMEKRIKKLLIPYG"
     gene            618..965
                     /gene="rplT"
                     /locus_tag="PK710_16830"
     CDS             618..965
                     /gene="rplT"
                     /locus_tag="PK710_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOT11450.1"
                     /translation="MTRAKRGFKARRRRNRILNQAEGFFGSRHKLFRQANEVVHKSWV
                     YAYIGRRLRRRDMRRLWIARINAGARLNGTTYSKLIAGLKKAQVELDRKILADLAVAD
                     PKGFSAVVKLACP"
     gene            1014..1793
                     /locus_tag="PK710_16835"
     CDS             1014..1793
                     /locus_tag="PK710_16835"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT11451.1"
                     /translation="MLTEPVTILFVHAFPLDARMWRPQLEAFRTYRVLAPDLRGFGRN
                     QRTPLFESVEQHAKDLIAVLDQHGVVHALVVGLSMGGYIALAMARLFPHRVAGLVLAN
                     TRAEPDSSEEQAQRVEMIAQVHHVGTSLLPNVMLPKLVAPACGDTTRNFLRMMMLEQP
                     SAGVVAALRSLRNRPDARGGLFQIHCPIALVGGQLDILTPPSMQQSIADEVPGARLTI
                     LPNAGHLTNLEASVAFNQVVMDTYERVRQTAYMERRKAPLR"
     gene            complement(1798..1989)
                     /locus_tag="PK710_16840"
     CDS             complement(1798..1989)
                     /locus_tag="PK710_16840"
                     /inference="COORDINATES: protein motif:HMM:NF024081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HOT11452.1"
                     /translation="MDWQSLIVALIVLLAVGYLAWRTVRSFKRPGCGGACDGCSERNQ
                     SCSEPSKLVQLRLKNTKKP"
     gene            complement(1993..4221)
                     /gene="feoB"
                     /locus_tag="PK710_16845"
     CDS             complement(1993..4221)
                     /gene="feoB"
                     /locus_tag="PK710_16845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOT11453.1"
                     /translation="MRSAPPRGELPVVALAGNPNAGKTSLFNALTGQTQHVGNYPGVT
                     VEKTSGIASAEGRRIEVVDLPGTYSLTAYSIEEIVTRDFVLQEKPDVVVDVIDSTNLE
                     RNLYLLLQFQELGVPVVGALNMADEAESKGIHIDIPLLSKLLGIPLIRTIGSRGVGKI
                     ELLQAVFDVLDGRVDTSERHLNYGPELEEKHEELMLVLRSDDVFANRYNLHWTAIKLL
                     EEDVDAVAKVRAEHAHADTVLDAAKTAVEWVRKHFGQDAEVVVGEQRYAYIRGALREA
                     VRTDPKPDGAKDSTELIDSIVLNRYLGVPIFFFVMLAIYALTFLAGNPLANVVESSVG
                     WLGSTVGTLLPEGILRDFLVNGVIAGVGGVLVFFPIVVLLFLGLSFLEDVGYMARAAF
                     VMDKFLHLFGLHGRSFIPLMVSTGCAVPGVMSARTLVNPKDRILTVLISPLMMCGAKT
                     PVVAMLCAAFFSRHAAIVFWALWLFGWAIAFITGFLFRKTLFRGEDSPFVMELPPYRI
                     PTLGGVASHMWRRSFSYVKKAGTFILAASIIIWALLTFPKADHVKTSFEQQVQTIELT
                     SAEQLATPDLPDQRRQQIERERDERIDSLRAECRMREAEFSIAGRLGKMVEPLVRPAG
                     FDWKISVALFSGIAAKEVIISTMGIVYGIGEADPDAEGNDSDASPLKDRLRADKAMSP
                     AVALSLMVFVMVYVPCIATLAVTRKELGSFKWPAFMAGFTLVVAWILAVAVFQTARLL
                     SS"
     gene            complement(4214..4444)
                     /locus_tag="PK710_16850"
     CDS             complement(4214..4444)
                     /locus_tag="PK710_16850"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOT11454.1"
                     /translation="MTLDQMQDGLPFVIDTLHSQGELRRRLVDMGFIAGAEGTVLRRA
                     PLGGPIELRIGRSRLALRLTEAQLVSVIPSHA"
     gene            4635..5450
                     /locus_tag="PK710_16855"
     CDS             4635..5450
                     /locus_tag="PK710_16855"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT11455.1"
                     /translation="MSPLPMKILLTGIPGPVSEAVLLELRKEPGLFEVHGVGSLSEPW
                     TYADRVAGRALPPDRYHIADLCNFHDFLKIVEGMDIVIHLDDGVTDAQESSRAAVYNV
                     FEASRQAAIRRVIYGSSAMVCLGYLQEEPYRSIRDHRWGDLPMALPMVTKTSVPRPID
                     RVASGKLWGEALGRIFADVHGLSVICIRWGWLEDPLNHPRAELSSLWCSRRDAAQLVK
                     RCVLAPNDVKFDVFFGVSANHRRWVDIDDARSRVGYRPIDSVEEHGTMRIPAA"
     gene            5599..>6076
                     /locus_tag="PK710_16860"
     CDS             5599..>6076
                     /locus_tag="PK710_16860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11456.1"
                     /translation="MVIDREKILQDAQKLVSKGRLDRAVIEYQKLIKANPSDDRVMLK
                     VADLQTRMRAYEAAVTTYEQVASYYAAQGFSAKATAIYKQIRSLIQQHLPETKGHYGP
                     VVDKLVQLYLEAGLKGDALSTYQEYAAHLQQAGRDAELPAIYRKIVELKEDDLDSRR"
BASE COUNT         1352 a         1589 c         1801 g         1334 t
ORIGIN      
        1 caatcgaaca tcgagcaaaa accgctgata acgacgcaac caggtacctt cgatgagggg
       61 ttggggatac cgcatgggat tcggaagatg ataaacggga cccacgcgtt catggcgaaa
      121 agctcggggt tcggatggcc ggggatacgc tcgcgaaaat agcctcgagt gtctgcgggt
      181 gcttgtgagc gcgtgtttcg gtaagggtcg agcccgagcg tgttcatctt gacaacctat
      241 tttttgtcga ccataacctg cgctccgctt ttctgatggt tcgaacagct ttcgcctcct
      301 gcgtgaaggt ggcctcgaga aagcggattt ggtccgcggg acccctgctc gttccgcatt
      361 gcttctgaaa gagccctgtc gatggcaccc aaaattaaaa tgaggaccaa tcggtccgcg
      421 tccaagcgct tccgcttgac gggtaccggt aagattcgtc gcagcaaaag tggtggtaac
      481 cacggtttga gcaacaagaa ccgcaaacgg ctgcgccgcc tgcgtgacaa cgacactgtc
      541 gcttcatgca tggaaaagcg gatcaaaaaa ctgctcattc cttacggctg agcccgaatc
      601 tgaccaggag ttgaccgatg acccgagcaa aacgtggatt caaggcgcgc cgtcgccgca
      661 accgtattct caaccaagcc gaaggttttt tcggctcgcg tcacaagctt ttccgacaag
      721 ccaatgaggt tgtccataag tcgtgggtct atgcctacat tggacgccgc ctgcgccgtc
      781 gtgacatgcg acgtctttgg attgcccgca tcaatgcggg agcgcgcctg aacggcacga
      841 cgtattccaa actcattgca gggctgaaga aggcgcaggt cgaactcgat cggaaaattc
      901 tcgcggatct cgcggttgcg gatcccaagg gattttctgc ggtcgtaaaa ctcgcgtgtc
      961 cgtgagttcg acgctgcctt gaccccttcg tctccttctt tccctccagc accatgctca
     1021 ccgaacccgt caccatcctt tttgttcatg cgtttccgct tgacgctcga atgtggcgtc
     1081 ctcaactcga agcctttcga acgtatcggg ttctggcgcc ggatcttcgg ggttttgggc
     1141 gaaaccaacg gacaccgttg ttcgaatcgg tcgaacagca cgccaaagac ctgattgcgg
     1201 ttctggatca acatggcgtc gttcatgcgc tcgtcgtggg tctgtcgatg gggggatata
     1261 tcgctttggc gatggcaaga ttgttccccc atcgcgtagc ggggctggtt ttggcgaaca
     1321 cgcgcgccga gcctgacagc tcggaagaac aagcacaacg cgttgaaatg atcgcgcaag
     1381 tccatcacgt tgggacctca ctgctaccga atgtgatgct tcccaagctc gtggcaccag
     1441 catgtggaga tacgacgcgg aatttcttgc gcatgatgat gctcgaacaa ccttccgccg
     1501 gggttgtcgc cgcgttgcgg tctttgcgca atcgtccgga tgcacgggga ggtctattcc
     1561 agattcattg ccccatcgcg ttggtgggag gtcaactcga tattctgact ccgccgtcga
     1621 tgcagcaaag cattgccgac gaagtcccgg gtgcccgctt gacgattctt ccgaacgctg
     1681 gacacctcac caacctcgaa gcgtccgttg cgttcaatca ggtcgtcatg gatacctacg
     1741 aacgcgtgcg acaaaccgct tacatggaac gtcgcaaggc accgcttcga taatccttca
     1801 cggttttttt gtgtttttga ggcgtagttg aacgagtttg ctcggctcgg agcagctttg
     1861 atttcgttcc gagcaaccgt cgcaagcgcc accgcatcct gggcgtttga agcttcgaac
     1921 ggttcgccag gccaggtagc cgacggcgag aagcacgatc aaagcgacga tgaggctttg
     1981 ccagtccatg gttcaactcg acagcagtct tgcggtttga aaaacggcaa cggcgagaat
     2041 ccacgccaca acaagtgtga agcctgccat gaatgcgggc cacttgaatg atcccagctc
     2101 ctttcgtgtc accgccaggg tagcgatgca cggcacgtac accatgacga agaccatgag
     2161 agacagggcg acggcggggg acatggcttt gtcggcgcgc aagcggtctt tcaggggtga
     2221 ggcatcggag tcattgcctt cggcatcggg gtcggcttcg ccgatgccgt acacgatgcc
     2281 catcgtcgaa atgatcactt ctttcgccgc gataccggaa aacagggcca ctgatatttt
     2341 ccagtcgaag cctgcaggac gaacgagcgg ctcgaccatc tttcccaggc gaccggcgat
     2401 gctgaattct gcttcgcgca tgcggcattc ggctcgaagc gagtcaattc gttcgtcacg
     2461 ttcgcgttcg atctgttggc gacgctggtc gggaagatcg ggggtggcaa gttgttcggc
     2521 ggaggttagc tcgatggttt gcacctgttg ttcgaagcta gttttgacat ggtcagcttt
     2581 gggaaacgtc aatagcgccc agatgatgat cgatgcggcg aggatgaagg ttccagcttt
     2641 tttcacgtac gaaaaggagc gtcgccacat atgggaagcg acacctccca gggttggaat
     2701 tcggtaggga ggtaattcca tgacgaaggg ggagtcttcc cctcgaaaca gggtttttct
     2761 gaaaaggaac ccggtaatga aagcgatggc ccatccgaac agccagagag cccaaaacac
     2821 gatggccgcg tgacgagaga aaaaggcggc gcacagcatg gcgacgacgg gagttttggc
     2881 gccgcacatc atgagcggtg agatcagcac ggtgaggatc cggtctttgg gattgaccaa
     2941 ggttcgcgcg ctcatgacac cgggcacggc gcaaccggtg gaaaccatca atgggatgaa
     3001 gcttcggcca tggagcccga acagatggag aaatttgtcc atcacgaagg cagcgcgagc
     3061 catgtatccg acgtcttcga ggaaagagag gccaagaaac agcagcacga cgatcggaaa
     3121 gaaaacgagc acgccaccga ctcccgcaat gacgccattg accaaaaaat cgcgcaggat
     3181 tccttctgga agcagcgttc cgacggtgga tccgagccag ccgacggacg attccaccac
     3241 attggccagg gggttgccgg ccaagaaggt caacgcatag atcgcgagca tcacgaagaa
     3301 aaagatcggc acaccgaggt atcgattgag cacgatggag tcgatgagtt cggtggaatc
     3361 tttggcgcca tcgggctttg ggtccgtgcg aacggcctcg cgcaaggcgc cgcgaatata
     3421 ggcgtatcgc tgctcgccca ccacgacttc ggcatcttgt ccgaagtgtt ttcgcaccca
     3481 ttcgacggcc gtttttgcgg catcgagcac cgtatcggca tgagcgtgtt ccgcgcgtac
     3541 tttcgcgaca gcatccacat cttcttcgag gagtttgata gcggtccaat gaaggttgta
     3601 tcgattggcg aaaacgtcat cggaacggag aacgagcatc aattcttcgt gtttttcctc
     3661 gagttcgggc ccgtagttga ggtgtcgctc gctggtatcc acacgcccat cgagcacatc
     3721 gaaaacggct tgaagcaatt ctattttacc caccccccgg ctgccgatgg tacgaatcag
     3781 ggggatccct aaaagcttcg aaagtagcgg aatatcaata tgaattcctt tactctccgc
     3841 ctcatcggcc atattgagag cgccgacgac ggggaccccg agttcctgga actgcaggag
     3901 caagtacagg ttgcgttcga ggttggtgga atcgatcaca tccaccacga catcgggttt
     3961 ttcttgaagc acgaagtccc gggtgacgat ttcctcgatc gaataggcgg ttaggctata
     4021 ggtgccggga aggtcgacga cttcgatgcg tcgaccttca gcgcttgcga taccggaagt
     4081 tttttcgacc gttacgcccg gatagttgcc aacgtgttgg gtttgcccgg taagtgcgtt
     4141 gaacaagctg gttttccccg cgttgggatt gccagcgaga gcaaccacgg gcaattcgcc
     4201 acgaggtggg gcgctacgca tgggaaggga tgacggaaac gagttgtgct tctgtgaggc
     4261 gaagggcgag tcgcgagcga ccgattcgca gttcgatggg tccgccgagg ggggcgcgac
     4321 gcagcacggt gccttcggcg ccggcgatga atcccatatc gacgaggcgt cttcgaagtt
     4381 cgccctggga atggagggta tcgatgacga agggcaggcc gtcttgcatc tggtcaagag
     4441 tcatgagggc acgattcgcg ggtttgacgg gatggtgaag gtcttttcgt ggatgtaatt
     4501 ccgattgtga gtaacgtcaa tagttagtag acgtctctaa caagatattc tcgatcgata
     4561 ccgatttttt ggcgattgtc aggtcaagga gtaggggaag ggagagcgat agcgtgggga
     4621 gaaaggagtc caccatgtcc ccattaccca tgaaaatcct actcacaggc attcctggac
     4681 ccgtttccga agcggttttg ttggaactaa ggaaagaacc ggggcttttc gaggtgcatg
     4741 gggttggttc cctttcggag ccgtggacgt atgccgatcg ggttgcgggg cgtgctttgc
     4801 cgccagatcg ttatcatatt gcggacctgt gcaatttcca tgattttcta aaaatagttg
     4861 aaggcatgga tattgttatt cacttggacg atggtgttac cgacgcgcag gagagcagcc
     4921 gggccgccgt gtacaacgtt ttcgaagcga gtcggcaagc ggcgatacgc cgtgtgatct
     4981 atggttcctc ggccatggtt tgtctgggtt atctgcagga ggaaccgtac cgatccatcc
     5041 gagaccatcg ttggggcgac ctgccgatgg cgctaccgat ggtcaccaaa acgtcggtgc
     5101 caagaccaat cgatcgggtg gcgagtggca aactctgggg cgaagccctt gggcgaatat
     5161 ttgcggatgt gcatggttta tcggtgatct gcattcggtg gggctggctc gaggaccctt
     5221 tgaaccatcc tcgagcggaa ctatcttcct tgtggtgcag ccgtcgtgat gccgctcaac
     5281 tggtaaaacg atgcgtacta gctcccaacg atgtgaaatt cgatgtgttt tttggagtat
     5341 cggcaaacca tcgacggtgg gtggacattg atgatgcccg aagccgtgtg ggctataggc
     5401 ccatcgacag tgtggaggag cacggcacga tgaggatccc ggcggcttga acgacccatc
     5461 gttgggcttc gcgaaatacc tttggtgttt cgggtaactt cgttcggaga tcttgacgta
     5521 gtgcagggtt ggtcgctaag tcagaaccct catggtttgt cttccgtgga tcggaaggta
     5581 tgacgagcaa tcggcaccgt ggtaattgat cgcgaaaaga tacttcaaga cgcccaaaag
     5641 cttgtgagca aggggcgatt ggatcgcgct gtcatcgagt atcagaagct catcaaggcg
     5701 aacccgagtg atgaccgggt catgctcaag gttgcggact tacaaactcg catgcgcgcg
     5761 tacgaagccg cggtaaccac atacgagcag gttgcctcct attacgccgc ccaaggtttt
     5821 tcggccaaag cgacagcaat ctacaaacag attcgctcgc tcatccagca acatctaccc
     5881 gaaaccaagg ggcattatgg gccggttgtc gacaaactcg tgcaattgta tctcgaagcc
     5941 ggtctcaaag gcgatgcgct cagcacctac caggaatacg ctgcccatct acaacaagct
     6001 ggtcgagatg ccgagttgcc cgccatctat cgaaagatcg tcgaactcaa ggaagatgat
     6061 ctcgatagtc gtcgca
//
LOCUS       DAOSOR010000350         6072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13127_length_6072_cov_4.127140, whole genome shotgun sequence.
ACCESSION   DAOSOR010000350 DAOSOR010000000
VERSION     DAOSOR010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13127_length_6072_cov_4.127140"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PK710_16865"
     CDS             <1..532
                     /locus_tag="PK710_16865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11457.1"
                     /translation="GAQATVNLIGYFHRFDTGFLYTKVPTMAAFSLGAKYDQRSIERY
                     PAGNAQLAFRSGIFSLESEFYGKRELEFKSTQFAYNVTGGLLLVPKHLFFAADYGAFI
                     AGKLEKPPEDLFDTGSDVRKQRDEMQARAALHWFFYRHVGVLSALYRYRDVKSSRDQK
                     DGYKESEGRLVASYWF"
     gene            complement(647..2914)
                     /locus_tag="PK710_16870"
     CDS             complement(647..2914)
                     /locus_tag="PK710_16870"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOT11458.1"
                     /translation="MLLPHFVALVAVSLVSCSPSYSHPQIHASPSCTETTSKPESPTP
                     GNGSWFMPPPASGEGMWPWHDLSRLDESKLRHRGLAVSLSDLWTPGKGGLLRAVAGLQ
                     GCSASFVSSDGLLITNHHCVHRAIQRNSTPGRDYLSDGFVAKTRADELPGLGTLVYVF
                     QRQSDVTSRILDTLDSSLTDLQRLKEIERRESMLVRECESKPNMRCEVARENDGLRFM
                     MLENTELKDVRLVAFPPESLGNYGGEIDNWHWPRHTLDFAILRAYVKPSGEPAEYDGA
                     NVPYHPDTYLTVSELFPTPPSLVMVAGKPGKTRRYAHHTEVEDALEWYYPFRDSLYTA
                     WIEALERGAGDDPAARLVVSSWLRRLNNGLFHARGMIKGLRANGAVERARRRDEALRA
                     WISEKPERAARYASVLDDLENHLRASRETRQKDQLLGALVSGAQVFGFSRRIVKWAAQ
                     REKPDDVREPGYQDRDRDTVRRELEQAQRSLHLDSDRAVMRLMVQRLCALAPHERPGA
                     LLGPEGLPCEADQLQKKLRELYQGTKLGMIQGRLDAFGLGLDELRRSKDPMIQLALRV
                     SQEFDDLDRRNQEREGARIRLERPYLQALIAFRGKAFYPDANSTPRISFAHVAGYAPQ
                     DGVWHIPQTTFRGMLDKHTGSPPFQVPEMLFRAFENQAFGQYADPSGADVPLCFLSNA
                     DTTGGNSGSPVLDGTGKMVGLNFDRVFENIAGDYGYNPDRSRNVMVSTRAILWYLQHV
                     VQAYPLVSEIRSTHP"
     gene            3058..3714
                     /locus_tag="PK710_16875"
     CDS             3058..3714
                     /locus_tag="PK710_16875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11459.1"
                     /translation="MDADESRSNLDLEGRCGTCARFVRVTERVEAHGAVSRVGDCLLG
                     VWPSPLNERGTCPHYTKRGEFKLSLSTKNQRTATRTPKRTSPVVAQPFSFPEAMTDMD
                     ADEFRLALRQVLREELGLGEVTIAPRWAGGQVIVKPGRDGVAEKTIPIDSLFHKIVLI
                     RDKLRVLEQKINSHPLLTDEDKVQMQQYITQCYGSLTTFNLLFDAKDEGFVGQRNREE
                     "
     gene            complement(3862..4128)
                     /locus_tag="PK710_16880"
     CDS             complement(3862..4128)
                     /locus_tag="PK710_16880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11460.1"
                     /translation="MGTVLSPRSGFVGDIDNIFIKWAMRIGFVASHRTFLVKKSLLAS
                     ESVQQQRDLFALVLPQHFDKALVGDIRRVIRAFSGWNTISAPWL"
     gene            4177..>6072
                     /locus_tag="PK710_16885"
     CDS             4177..>6072
                     /locus_tag="PK710_16885"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOT11461.1"
                     /translation="MKNTPALTEASVRQVYERQAQRHEWARNLLGRPLTLAEKILFAH
                     VKPYPDAAPKDFTRLKSSTQLWPDRVTLQDATAQMAILQFMQAGVPRVAVPSTVHCDH
                     LIQAQQGAQKDLLRAFDENQEVYDFLASASKKYGLGFWQPGAGIIHQVVLENYAFPGA
                     LIIGTDSHTPNGGGLGMLAIGVGGADAVDVMVGAPFGLRWPGVIGVRLTGKLSGWTAP
                     KDVILHLMGILTTRGGTGHIVEFFGPGTASLSATGKGTICNMGAEHGATTSVFPFDQA
                     MAVYLRATGREAIAALAEQHASELRADKQVEDDPERFYDRVIEIDLDTLEPHINGPHS
                     PDRAYPISKLAEAIASESWPDKLSATLIGSCTNSSYEDMVRSAAVVQAARAQGLKLAS
                     PLYVTPGSDQIFETLKRDGLLEIFENAGALVLANACGPCIGQWKREDIVQGQENTILN
                     SYNRNFPRRNDGNPATLSFIGSPEIVTALAFAGRLSFNPMKDTLVAADGKTVRLQAPS
                     PAENGGLPKAGFAHGLAGFIEPAEDGNDLEVLIDPKSKRLQRLEPFAPWDGKDFENLV
                     VLLKATGKCTTDHISPAGPWLRFRGHIDAISDNMFLGANNAFTDKPGTAVSALSGENE
                     VPIAALAR"
BASE COUNT         1390 a         1890 c         1564 g         1228 t
ORIGIN      
        1 cggcgcgcaa gccaccgtca acctcatcgg ttacttccac cgcttcgaca cgggatttct
       61 ctacaccaaa gtgcccacca tggccgcatt ctcgctcggc gccaaatacg accagcgttc
      121 catcgaacga tatcccgccg gcaatgccca actcgctttc cgttccggta tcttcagtct
      181 cgagtccgag ttctacggca aacgagaact cgaattcaaa tccacgcaat tcgcctacaa
      241 tgtcaccggc ggactgctcc tcgtgccaaa acacctgttt ttcgcagcag actacggcgc
      301 cttcatcgcg ggcaagctgg aaaaaccacc agaagacctc ttcgatacag gtagcgacgt
      361 tcgcaagcag cgcgatgaaa tgcaagcccg tgccgccctc cattggttct tctatcgaca
      421 cgtcggcgtg ttgtcagcct tgtaccggta tcgcgatgtc aagtcctcac gcgatcagaa
      481 agacggctac aaggaaagcg aaggccgact cgtagcaagt tattggttct aacccatctg
      541 agtctctttc caatctctcc gccgcgcctc ccgcttcagt cgacctctgc ccacaaggtg
      601 ttgcttctgg cagcccttcg aactcatgga tgaccagatc gcgttgtcag ggatgcgttg
      661 atcgaatctc cgaaacgaga ggatatgcct gcacaacatg ctgcaggtac cataaaatcg
      721 cgcgagtact caccatcaca ttgcgcgaac ggtcggggtt gtacccgtag tctcccgcta
      781 tattctcgaa cacacgatcg aagttcaacc ccaccatctt gcctgtccca tcgagcacgg
      841 ggctgcccga attaccgcct gtcgtatccg cattggacaa aaaacataag ggaacgtcgg
      901 caccggaggg gtcggcatac tgcccaaacg cttgattctc gaacgcgcgg aaaagcatct
      961 ccggaacttg aaagggagga ctgcccgtat gcttgtcgag catccctcga aacgtcgttt
     1021 ggggaatatg ccaaactcca tcctgcggag cgtagcctgc cacatgggcg aaactgatac
     1081 gcggtgtgct attggcatcg ggataaaacg ctttgccgcg aaacgcgatc aaggcttgca
     1141 aatatggacg ctcgagacga atcctcgcac cttctcgctc ttgattgcga cgatcgaggt
     1201 catcgaattc ttggctcacc cgaagagcaa gttggatcat tgggtctttc gaccgccgaa
     1261 gctcatccaa ccctaggcca aacgcatcga gacgcccctg gatcatccca agcttggtgc
     1321 cttgatataa ctcccgcagc tttttttgaa gttgatccgc ttcacacggc agtccttctg
     1381 gcccaagcaa cgcaccggga cgttcgtggg gagccagggc gcaaagacgt tgaaccatga
     1441 gccgcatcac ggctcggtcg gaatccaggt ggaggcttcg ttgcgcttgc tcgagttccc
     1501 gacgaaccgt gtcacgatcg cgatcttgat aaccgggctc tcgcacgtcg tccggtttct
     1561 ctctttgcgc cgcccacttc acgatccgtc gcgaaaaacc gaatacctgt gcccccgata
     1621 ccaaagcacc gagcagttga tctttttgtc tcgtttcgcg cgatgcccga aggtgattct
     1681 ccaaatcgtc gagcacactt gcataccgcg ccgctcgctc gggcttttcc gagatccacg
     1741 ctcgaagggc ctcatctcgt cttcgcgcgc gttcgaccgc gccgttggcg cgcaaacctt
     1801 ttatcatgcc acgagcgtga aatagcccat tgttgagacg tcggagccac gacgatacga
     1861 caagacgagc tgccggatca tcgcctgcgc cccgttccaa cgcttcgatc caagcggtat
     1921 acagactatc ccgaaatgga taataccatt caagggcgtc ttctacctcg gtatgatgcg
     1981 catatctacg cgtttttcct ggttttcctg caaccataac cagggatggc ggagtgggga
     2041 acaattccga tacggtcagg taggtatctg gatgataggg cacattggca ccgtcgtact
     2101 ccgctggttc gccagagggt ttgacataag cccgcaatat ggcgaaatcc aacgtgtgtc
     2161 gcggccaatg ccagttgtcg atctcaccgc cgtaattgcc caagctctcc ggcggaaaag
     2221 ccacgagacg aacatccttc agctccgtgt tttcgagcat catgaaccga agtccgtcgt
     2281 tttctcgcgc cacttcacag cgcatattgg gcttgctctc acactcgcgt acgagcatcg
     2341 attcccgacg ctcgatctct tttagccgtt gcaggtcggt caacgacgaa tccaaggtgt
     2401 cgaggatgcg agaggtcacg tccgattgcc gttgaaacac gtacaccagg gttcccaaac
     2461 cggggagttc atcggcgcgt gtcttggcga cgaaaccatc ggacaggtaa tcccgaccgg
     2521 gtgtcgaatt acgctgaatg gcccgatgaa cacaatgatg gttcgtgatg agcaagccat
     2581 cagaggaaac gaacgaagcg ctgcatccct gcagcccggc caccgcgcga agcagcccac
     2641 cttttccggg tgtccaaaga tccgaaaggg aaaccgccaa acctcggtgg cgcaacttcg
     2701 actcatcgag tcgtgataga tcgtgccacg gccacatgcc ttcccccgac gcgggcggtg
     2761 gcatgaacca ggagccgtta cccggagtcg gactctcagg cttcgacgtg gtctcggtac
     2821 aggacggcga cgcgtggatc tgtggatggg aatacgaggg ggagcaactc acgagggata
     2881 ctgccaccaa agccacaaaa tgcggcagca gcatgggcaa tctctttgcg aaacggacgc
     2941 gcatcttcgc gatagcctaa tccgaacccc accctgtggc gacccgaacc atctccgatg
     3001 ggttctgctg ttttcctgcc ttgacacacg accccgtatg ccattgccta tcgcgtcatg
     3061 gatgctgatg agagccgatc caacctcgat ctcgaggggc ggtgtggaac ctgcgctcga
     3121 ttcgttcggg tcaccgagcg ggtggaggcc cacggtgcgg taagtcgtgt cggcgactgc
     3181 ctgcttggcg tgtggccttc gcccttgaac gaacggggaa cctgtcccca ttacacgaag
     3241 cgaggcgaat tcaagctctc cttatccaca aaaaatcaac gaaccgccac tcgaacccca
     3301 aaacgaactt cgcccgtggt ggcgcaaccc ttttcgtttc cagaggcaat gaccgacatg
     3361 gatgcagacg aattccgctt ggcgcttcgg caagttctcc gcgaggaatt aggcctgggc
     3421 gaggtgacca tcgctccccg ttgggcggga gggcaagtca tcgtcaaacc ggggcgcgat
     3481 ggagtagcgg aaaaaacgat ccccatcgat agcctttttc acaaaatcgt gctcatccgc
     3541 gacaaactcc gggtcctgga gcaaaaaatc aacagccacc ccctcctgac cgatgaagat
     3601 aaagtgcaaa tgcagcagta catcacgcaa tgctacggct cgctcacaac cttcaacttg
     3661 ttgttcgacg caaaagacga aggcttcgtc ggacaacgta atcgtgagga gtgagcgaac
     3721 gacccgctca attacaaaat gggaaggaac acgcgcaacg gcagacagcc gcgtatcatc
     3781 ccatcttcta ggatggctgc tcaatccagg aaatcaccga atctgaagct tgtttcgaat
     3841 tccgaggcga agcaccctcc gtcacaacca cggtgcgctt attgtgttcc agccgctaaa
     3901 ggctcgaatg acccggcgga tatcgccgac gagtgcctta tcaaaatgct ggggcaagac
     3961 gagcgcaaac aggtctcgtt gttgttgtac cgactcggac gcgaggaggg actttttgac
     4021 caaaaatgtc cgatgactcg cgacaaaacc tatacgcatt gcccatttta tgaagatatt
     4081 gtcgatatcg ccgacgaatc ctgaacgcgg cgacaagacg gtgcccactc cacctcagtc
     4141 ttgtggttcg acccaacacg ccccatgatg cggggcatga aaaatacccc agcgttgacg
     4201 gaagcttcgg ttcgacaagt gtatgaacgg caggctcagc gacatgaatg ggcccgaaac
     4261 ttactcggtc gccccttgac cctcgccgaa aaaatccttt tcgctcacgt caaaccttat
     4321 cccgacgcgg cgccgaagga cttcacgcga ttgaagtcct ccacccagct ttggccggac
     4381 cgcgtgacct tacaagatgc cacggcccaa atggcgatcc tccagttcat gcaggccggt
     4441 gtccctcggg tcgccgttcc ctccaccgtg cattgcgacc acctcatcca ggcccaacaa
     4501 ggagcccaaa aagacctgct ccgcgctttc gacgaaaacc aagaagtcta cgattttctc
     4561 gcaagcgcct cgaaaaaata cggcctcgga ttctggcaac ccggagcggg catcattcat
     4621 caagtcgttc tcgaaaacta cgctttcccc ggggcgctca tcattggaac ggattcccat
     4681 acccccaatg gcggcggcct cggtatgctc gccatcggcg ttggcggcgc tgatgccgtc
     4741 gatgtcatgg tcggcgctcc tttcggcctt cgatggcccg gcgtcatcgg cgtgcggctc
     4801 accggaaaac tatccggatg gaccgccccc aaagacgtca tcctgcatct catgggcatc
     4861 ctcaccaccc gcgggggaac cgggcatatc gtcgagtttt tcggcccagg caccgcaagt
     4921 ctttcggcca ccggcaaagg cacgatctgc aacatgggcg cggaacacgg cgccaccacc
     4981 tccgtatttc ccttcgacca agccatggca gtctatctac gcgcgacagg acgggaagcc
     5041 atcgctgccc ttgccgagca gcacgcctcg gaactgcgcg cggataaaca agtcgaagac
     5101 gatccggagc gattctatga ccgcgtcatc gaaatcgacc tcgataccct cgagccccat
     5161 atcaatggcc ctcattcccc ggaccgcgcc tatccgatct caaaactcgc cgaagccatc
     5221 gcttccgaat cctggccgga caagctttcc gctaccttga tcggaagctg caccaactcg
     5281 tcttacgaag atatggttcg ctccgcggcc gtcgtgcaag ctgcgcgcgc ccagggattg
     5341 aaactcgcct ccccgcttta cgtcaccccc ggctcggacc agatctttga aaccctgaaa
     5401 cgcgacggtt tgctcgagat cttcgaaaat gccggggcgc tcgttctcgc caacgcttgc
     5461 ggcccctgca tcggtcaatg gaaacgggaa gatatcgtcc aaggccaaga aaacaccatc
     5521 ctcaatagct acaaccgcaa tttcccgcgt cgcaatgatg gcaatcccgc taccttgagc
     5581 ttcattggaa gcccggaaat cgtcacggcc ctggcttttg ccggtcggct gtctttcaat
     5641 cccatgaaag acacactcgt ggcagccgat gggaaaaccg ttcggctaca agccccttct
     5701 ccagccgaaa atggaggcct gccaaaagcc ggattcgctc atgggctggc tggattcatc
     5761 gaacccgccg aagacggcaa cgatctcgaa gtgctgatcg acccaaaaag caaacgatta
     5821 caacgcctcg aaccgtttgc cccgtgggat ggaaaagact tcgaaaacct tgtcgtccta
     5881 ctcaaagcca caggcaaatg tacgacggac catatctccc ccgcgggacc atggttgcgt
     5941 tttcgaggac atatcgacgc catcagtgac aatatgtttt taggagccaa taacgcgttt
     6001 acggacaaac ctggcacggc ggtcagcgcg ttgagcggcg aaaacgaagt ccccatcgcc
     6061 gctctcgccc gt
//
LOCUS       DAOSOR010000351         6062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13162_length_6062_cov_4.929915, whole genome shotgun sequence.
ACCESSION   DAOSOR010000351 DAOSOR010000000
VERSION     DAOSOR010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13162_length_6062_cov_4.929915"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PK710_16890"
     CDS             <1..510
                     /locus_tag="PK710_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11462.1"
                     /translation="TPQFLRVTKAYEVLLGACGSSILQSRRASIQDGNVRALPARPLA
                     LAQFLYHLGWISWESVQHALRWQAKHRIRIGELAVQSGALTRQQLLRIVEHQRSKERW
                     GRCALRLGLVDASTLSRLVERQTRLQPPVGQYFVQHGLVSSERLAHALALQRQHNELI
                     RENTKPTER"
     gene            complement(555..642)
                     /locus_tag="PK710_16895"
     tRNA            complement(555..642)
                     /locus_tag="PK710_16895"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(606..608),aa:Ser,seq:gct)
     gene            complement(694..778)
                     /locus_tag="PK710_16900"
     tRNA            complement(694..778)
                     /locus_tag="PK710_16900"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(742..744),aa:Ser,seq:tga)
     gene            complement(887..2068)
                     /locus_tag="PK710_16905"
     CDS             complement(887..2068)
                     /locus_tag="PK710_16905"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT11463.1"
                     /translation="MGQRLFAWMAIVMGLTAHAVLVGTWWETKEAQAAKVRKRPRIRP
                     VQRRAATARPTPLPPIERTKLHESCPPEMVNVARRFCIDRWEAGVVDAQSGKPASPYY
                     PPHSRLAERMQQQWVQAFREELSEARQWIEEAGLTPPLALGVWGADPSQWLDAGIATA
                     DAQADATPWLVPSPEAPHGWVMLGDGGDAGRPRPVMLLPPLYPWQREETFRPQAVSKY
                     DNIPQGYVPGFVANEACRRAGKRLCREEEWVFACKGEKQNKYPYGDNYRQGQCNVFRE
                     DHPARILHGNWSVGLSDPRLNLIETPAGPLLRRTGTTSTCKSVWGQDAIFDMVGNLDE
                     WVDDPGGVFVGGFFSRSTRNGCEARVASHPPAYFDYSTGFRCCADLLGAAAAGVQDGR
                     P"
     gene            complement(2115..3249)
                     /gene="prfB"
                     /locus_tag="PK710_16910"
     CDS             complement(join(2115..3179,3181..3249))
                     /gene="prfB"
                     /locus_tag="PK710_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153822746.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOT11464.1"
                     /translation="MSAEIREKLADLQRRFQVLRRHLDAPKLSEELNKLNMQAGSAGF
                     WDDQDKAQKVLRRRAAVEQQLELMESLTRQLRDAEDYFELAEEENDAEALKDAEQQVN
                     DVEVAVRRAELRRMLSGPVDHADAIVSIHPGTGGTDAKDWAEMLLRMYLRWAERRGFK
                     TELIDRQEGDEAGIDSAAFTLQGENAFGYMRSEIGVHRLVRISPFDANARRQTSFAAV
                     EVTPDIEDEIQIDVRDEDLEITTMRAGGKGGQNVNKVETAVRLKHLPTNIVIVCRAER
                     SQHQNRRMALKMLKAKLFNMEMTKREEQAAAAAAAKSQIAWGNQIRSYVLQPYRLVKD
                     LRTEFETGNVDAVLDGDLDDLIEAFLLKAAGDTFKKGSQSVQE"
     gene            3506..3579
                     /locus_tag="PK710_16915"
     tRNA            3506..3579
                     /locus_tag="PK710_16915"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3538..3540,aa:Gln,seq:ttg)
     gene            3620..4612
                     /locus_tag="PK710_16920"
     CDS             3620..4612
                     /locus_tag="PK710_16920"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOT11465.1"
                     /translation="MPLPQLIITLGPASFDSLDELIRVGIHAVRLNGSHMDARTLEHA
                     VRRIRNTSPMGVIVDLQGAKMRFGTFVPRTIHAGQSLTFSHDAHDAHDAIPVVHPELY
                     RDAHVGDTLRCDDDRIRFRIDEVTSDHINVTCLSDGVLHPRKGVNVVEHPVHLDDLSH
                     SDVQQIEAVRPFDDITFACSFMRDGREAAWVRQRAPHAAIIGKVERAEAMDAIDSISA
                     SVDAVWICRGDLGAQVGLATMARWIGAFQPASLPVPTFMAGQVLEHLTHHANPTRSEV
                     CHLHDLLQRGYAGIVLSDETAIGLDPLRAASTAAALLKTLARGRNTRGRSGAEN"
     gene            4739..4815
                     /locus_tag="PK710_16925"
     tRNA            4739..4815
                     /locus_tag="PK710_16925"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4773..4775,aa:Arg,seq:tcg)
     gene            4852..4924
                     /locus_tag="PK710_16930"
     tRNA            4852..4924
                     /locus_tag="PK710_16930"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4885..4887,aa:Lys,seq:ctt)
     gene            5169..>6062
                     /locus_tag="PK710_16935"
     CDS             5169..>6062
                     /locus_tag="PK710_16935"
                     /inference="COORDINATES: protein motif:HMM:NF026052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOT11466.1"
                     /translation="MPHDAMPVAPRIYNLFPLLAGPIRNWAALLPNITAMGFDWVYLN
                     PWHYPGFSGSLYAVKDYDRVHPRLADDTQDPKIALKHFLQQAREYGLRVMMDLVINHT
                     SKDALLAHQHPEFYLRDDSGELVSPSAIDPADARKVTVWGDLAELDYRPRTERSALLG
                     IWKDLLRNSIDLGFSGFRCDAAYQVPADVWAELIDYGRKNSHVLFVAETLGGRLEPMK
                     QLASAGFDAFFNSSKWWDFRQPWLLEQYECFRHLAPSIAFPESHDTPRLIADLAEQSI
                     TDPWRIEAHYRLRYLFAAFFSA"
BASE COUNT         1200 a         1909 c         1597 g         1356 t
ORIGIN      
        1 acaccccagt ttttgcgcgt gacgaaggcc tacgaggtgc tgcttggagc ctgcgggtca
       61 tcgattcttc agtcgaggag ggcgtctatc caagacggca acgtgcgggc acttccggct
      121 cggcccttgg cgctggccca gtttttgtat catctgggct ggatttcgtg ggagtcagtg
      181 cagcacgcct tgcgctggca agcaaaacat aggatccgca ttggcgaatt ggccgtgcaa
      241 tccggcgcgc tcacacggca acaactcctt cgcatcgtcg agcatcaacg ctccaaggaa
      301 cggtggggac gatgcgcgtt acgtcttggg ttggtggacg caagcacgtt gtcacggctt
      361 gtcgaacggc agactcgatt gcagccgcct gtgggccagt acttcgttca gcacggattg
      421 gtatcgtcgg agcgattggc tcacgccttg gcgctacaac ggcaacacaa tgaattaatt
      481 agagaaaata caaaaccaac ggaacgctga tccggtgtct atccggcgcg ttgccctttg
      541 ctttcgcccc acagcggaga gggtgggatt cgaacccacg gtaggctttt gacctacgtc
      601 cgcttagcaa gcggatacct tcggccactc ggtcacctct cccgtaaccg gcctgggtac
      661 tgtccgtatc gcgatgcgct ggcctcacgg cagcggagga ggagggattc gaacccccgg
      721 taccttgcgg tacggcggtt ttcaaaaccg ctgccttcga ccactcggcc actcctccct
      781 catgggttca tccacatcgt gtggccgaaa cccgcggagc cggcctttcc caggctcgat
      841 attttcaaag aacggtcgct cgaactacct cgcaccgcgc tcgccgtcaa ggtcttccat
      901 cctgaacccc cgcggcagcc gcccccagca gatccgcaca acagcgaaat cccgtggagt
      961 agtcgaagta tgccggcgga tgggatgcca ccctcgcctc acagccgttg cgggtgctac
     1021 gtgaaaaaaa gcctcccaca aacacacccc ccggatcgtc gacccattca tcgagattgc
     1081 ccaccatgtc gaaaatggcg tcctggcccc acacgctttt gcacgtggaa gtggtgcccg
     1141 tgcgacgcaa caacgggccc gccggtgttt cgatcaggtt gaggcgcggg tcgctcaatc
     1201 ccacggacca attgccatga aggattcgcg cgggatgatc ttcccgaaac acgttgcatt
     1261 ggccttgtct gtagttatcc ccatagggat atttattttg cttttctccc ttgcaggcaa
     1321 acacccattc ctcttcacga cacagtcttt tccctgctcg acgacaagcc tcattcgcta
     1381 cgaatccagg tacatagcct tggggaatat tatcgtattt ggatactgct tgcggtcgaa
     1441 acgtttcttc tcgctgccaa gggtataacg gtgggagcag catcacgggc cgaggccgac
     1501 ccgcatcacc cccatcgccc aacatcaccc aaccatgcgg cgcctcgggg gaaggaacga
     1561 gccacggtgt ggcgtcggct tgcgcgtcag ccgttgcgat gcccgcatcg agccattggg
     1621 aagggtccgc tccccatacg cccaacgcca aaggaggcgt gagtcccgct tcctctatcc
     1681 attggcgcgc ctcggataat tcttctcgaa atgcttgcac ccactgctgt tgcatccttt
     1741 cggccaaccg cgagtgcggc ggataatagg gcgaagccgg cttgcccgat tgggcatcca
     1801 cgacccccgc ttcccaacgg tcgatgcaaa accgcctcgc cacattcacc atctccggtg
     1861 gacaggattc atgaagcttc gtgcgttcga tgggaggcaa tggcgtgggg cgagccgtgg
     1921 cagcccgccg ctgcaccgga cgaattcgtg gtcttttccg aactttggcc gcttgcgctt
     1981 ccttggtttc ccaccacgtt cccacaagca ccgcatgggc cgtcagaccc atgacgatcg
     2041 ccatccatgc gaagagtcgc tgtcccatga tgaaccgcgt gctcgtcgtt cgacgagcca
     2101 acgggcgaat tacgtcattc ctggacgctc tggcttcctt ttttgaaggt gtctcccgcc
     2161 gccttgagca aaaatgcctc gatgagatcg tccaaatcac catccaatac cgcgtcgaca
     2221 ttgccggttt cgaattccgt tcgtaagtct ttcaccagac gatagggctg caacacataa
     2281 gaacggattt gattgcccca ggcaatttga cttttcgctg ccgccgcggc cgccgcttgc
     2341 tcttcccgct tcgtcatctc catgttgaaa agcttggcct tgagcatttt gagcgccatg
     2401 cgacggttct ggtgttggct ccgttcggct cggcagacga tgacgatatt cgtggggaga
     2461 tgcttcagtc gcaccgccgt ttcgaccttg ttgacgtttt gaccaccctt gcctcccgca
     2521 cgcatggtcg tgatctccag atcctcgtcc cgcacatcga tctgaatctc gtcttcgata
     2581 tccggcgtca cttccaccgc cgcaaaggag gtttgtcgtc gcgcgttggc atcaaaggga
     2641 ctgatgcgca ccaggcgatg cacaccgatt tccgaacgca tgtacccaaa cgcattctca
     2701 ccttgcaggg tgaacgcggc cgaatcgatt cctgcctcat ccccttcctg ccgatcgatg
     2761 agttcggtct tgaatccacg acgttcggcc catcgcaagt acatgcgaag cagcatctcc
     2821 gcccaatcct tggcgtccgt tcctcccgtg cccggatgga tcgatacgat agcgtcggca
     2881 tgatccaccg gcccggaaag catgcgacgc aattccgccc gacgtacggc tacttcaacg
     2941 tcgttgactt gttgttcggc gtctttcaat gcctccgcat cgttttcttc ttccgcaagc
     3001 tcgaaataat cctccgcgtc tcgcaattga cgcgtcaaac tttccatcaa ctcgagctgt
     3061 tgctcgactg cggcgcgacg acgcaggact ttttgggctt tgtcctggtc atcccagaac
     3121 ccggccgaac cggcttgcat attgagcttg ttcagctcct cggatagctt cggggcgtca
     3181 aagatgcctc cttagcactt gaaagcgccg ttggaggtcc gcgagtttct cacgtatttc
     3241 ggctgacatg cgacgtatct aggcgatctt ttttcccttc gcaacgtcgg agaaatgccc
     3301 aagcgccgat gcccggtcgc gccatgccat gaggtttccc taggagcgct tgctgctgcc
     3361 ttcggccgac tcacaaaacc aaccttctcc caaccgtagg tcgtccctcc gataggtccc
     3421 cccgacagga cgaggaaaac gcagaaggct ccaattgtgc ttgcgaacag ggccaaggac
     3481 gaccatgctg tgcgccctgt tccgctccgg cgtcgtctaa aggcaggaca ggggactttg
     3541 gatcccctaa tcgtggttcg aatccacgcg ccggaaccaa ctccctgggc acgttcctac
     3601 tatgtcagcg tcccacaccg tgcctctgcc ccaactcatc attactctcg gccccgcttc
     3661 tttcgattct ctcgatgaat tgattcgagt cggaattcac gccgttcgcc tcaatggctc
     3721 ccacatggat gcccgcaccc tcgaacatgc cgttcgacgc atccgaaaca cttcccccat
     3781 gggtgtcatc gtcgatctgc aaggcgccaa aatgcgcttc ggaacctttg ttccacgcac
     3841 gattcatgcc ggacaatcgt tgacgttttc ccacgatgcc cacgatgccc acgatgctat
     3901 ccccgtcgta catcctgaat tgtatcgcga cgctcacgtg ggagatacct tgcgctgcga
     3961 cgacgatcgg attcgttttc gtatcgatga ggtgacctca gatcatatca acgtcacctg
     4021 cttgtccgat ggcgtcttgc acccgcgaaa aggcgtcaac gttgttgaac accccgtgca
     4081 tctcgatgat ctgagccatt cggacgtgca gcagatcgaa gcagtccgtc cgttcgacga
     4141 tatcaccttt gcttgttcgt tcatgcgaga tggtcgcgag gccgcatggg tgcgacaacg
     4201 cgcgcctcac gccgcgatca ttggaaaagt cgaacgcgcg gaagcgatgg acgccatcga
     4261 ctcgataagc gcatcggtgg atgcggtgtg gatctgtcga ggggaccttg gggcgcaagt
     4321 gggattggcc accatggcgc ggtggattgg agcctttcaa ccggcaagcc tgccggtccc
     4381 cacgttcatg gcgggccagg tgctcgaaca tctcacgcac catgcaaacc caacccgttc
     4441 cgaagtctgt cacctgcacg acctgctgca acgaggatac gccggcatcg ttctgtccga
     4501 cgaaaccgcg attggtctcg accccctgcg cgcggcatcg acggccgcgg cgttgctgaa
     4561 aacgctggct cgaggtcgaa atacgcgcgg acgatcgggt gcggaaaact aataaaacgt
     4621 tgtaaaatca gtatcttata taggattcaa caaagagcca ggcgattgga aaacgcggtt
     4681 gacagcaaga tttcacctcg ctacaagacg gctccgcgtc gctaaggcgc gaagcttggc
     4741 gcccgtagct cagctggata gagcgtcgga cttcgaatcc gcaggtcgcc cgttcgaatc
     4801 gggccgggcg cgccacggaa atagattttt tgataattct ttcgttttcc cgggttagta
     4861 gctcaattgg tagagcagcg gactcttaat ccggaggttc ggggttcgag tccccgctga
     4921 cccactgctt atcttcaagg gggttccttc gctggaaccc ccattttttt gtcccttcga
     4981 aatcgatccg ttttttccgc ttcctctctc gatcgatatc gcgctgccaa gaccaaacgg
     5041 tcccttatgc tttcgcatcg ttcgacaaaa gtccacgcgc tgctgaagac tccagcaatc
     5101 tagggcttcc ccgccaccgc acggtttgca gagcacctca acgtcacggt tgtctggcaa
     5161 gccaagccgt gccacacgac gctatgcctg tcgctccacg catctacaat ctctttccct
     5221 tgctagcggg gcccatccga aattgggctg ccctattgcc caacatcacg gccatgggct
     5281 tcgattgggt ttacctcaac ccatggcatt accccggatt ttccggaagc ctgtacgccg
     5341 tcaaagacta cgaccgcgtg catcctcgtc ttgccgacga tacgcaagat ccgaaaatcg
     5401 cactcaaaca cttccttcag caagctcgcg aatacggcct gcgcgtgatg atggacctgg
     5461 tgatcaacca cacgtcgaaa gatgcgctgc tcgcccatca gcatcccgag ttttacctgc
     5521 gagacgacag cggcgagctt gtgtctcctt cggcgatcga ccccgccgat gctcgcaagg
     5581 tcaccgtatg gggtgacctg gcagaactcg attatcgtcc tcgcaccgag cgaagcgcgc
     5641 ttttggggat ttggaaagac ctgctacgaa acagtatcga ccttggtttt tcgggctttc
     5701 gatgcgatgc ggcgtatcaa gtgcccgcgg acgtatgggc cgaactcatc gactacgggc
     5761 gaaaaaacag tcacgtactc ttcgtggccg aaaccctcgg tggccgtctc gagccgatga
     5821 agcaactcgc aagcgcgggc ttcgatgcgt tcttcaacag ctcgaagtgg tgggatttcc
     5881 gacaaccctg gttgctcgaa caatatgaat gctttcgtca tctcgccccc tccattgcgt
     5941 ttcccgagag ccacgatacg cctcggctca tcgccgatct tgccgaacaa tcgatcaccg
     6001 acccctggcg aatcgaagcc cattaccgtt tgcgttacct gttcgcggcc tttttctcgg
     6061 cg
//
LOCUS       DAOSOR010000352         6051 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13195_length_6051_cov_4.298532, whole genome shotgun sequence.
ACCESSION   DAOSOR010000352 DAOSOR010000000
VERSION     DAOSOR010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6051
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13195_length_6051_cov_4.298532"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1494)
                     /gene="rimO"
                     /locus_tag="PK710_16940"
     CDS             complement(115..1494)
                     /gene="rimO"
                     /locus_tag="PK710_16940"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOT11467.1"
                     /translation="MLTFHITSLGCPKNTVDAEIILGNATARGLVPVENPADADVLIV
                     NTCGFIEPAKKESIDTIFQLSRFKQIGRCQRLVVTGCLAQRYAEQLASQIPEIDFLLG
                     TGDVLHVADAIVGQGPRLRVGSPQGFLSTASTPRMLSKVGSSAYIKIADGCSRQCAFC
                     AIPRIKGPHRSRSVEDIVQEAENLVSQGVIEINLVAQDTLRFGHDLSSTSSLATLVQR
                     LANVPGLHWVRLLYLYPDELDDALLELIAHHPHVAPYIDMPMQHAADPVLKTMRRGHG
                     QRRLRAIVERIRKRAPHASLRSAFIVGFPGETKEDFDQLVSFVQWARFDHLGVFRYSD
                     EEDTAAFDLPDKVPPRESYRRFRKLMSVQRPIARANNRQKKGQILQVLVEGPSEEHEW
                     VFAGRHAGQAPDIDGHVLFSESDVQPGEIWSAEVLTSSDYDLVVRIQGDTPIAKARLK
                     KILPVLPSR"
     gene            1600..1683
                     /locus_tag="PK710_16945"
     tRNA            1600..1683
                     /locus_tag="PK710_16945"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1634..1636,aa:Leu,seq:cag)
     gene            complement(1995..>5125)
                     /locus_tag="PK710_16950"
     CDS             complement(1995..>5125)
                     /locus_tag="PK710_16950"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HOT11468.1"
                     /translation="GGAGTGGSGTDSGSTDGHEQGETFIPTGGTGGAGGSETDPCAGF
                     YCPNDQHCESTNGSPTCMPNDCETLDCSPTEVCVETATGAYCADNSCTDDIDCLPEQY
                     CNGSICVPDICTPGDRHCQGANVIECAPNGGSEYVRHTCGGGSPYFTSECDDTLGACT
                     CEDDWDCPEYTTCEAGICVGTGIKPTCSLPPEPFENVLPVNEITWGGTQANPVAKESP
                     FPKSVQVVHVPIVANLDDDNGDGLIDERDFPEIIFTSFCNSDFTSNGTLRAIHGGGPN
                     KGKDYFASCGSKTWNEGAPIDSVPCTCAEADIDSTASLAVGDLDNDGIPEIVAVLEGN
                     GSNAPVRIYDNRGHIITESPTFANGGPNPAPTLANLDNHGFAEIVVGRTVWTLQHDAN
                     GKLAFANKFVGNLNAGINAQGPVSCVANIVGGQGLEVVAGSTAYRMPKGPAGLTLQSE
                     CTGSETDPDEVAWCKGQLVTVWDGQTVNGAAALPNDRKDGFCAIADVMGADFVLATGP
                     ANPLDGIPEVITINAGFLNVFDGPTGKLRLSLDLKAGGGGGPPNVDDFDGDGFPEVGT
                     ALGAAYVVIDFQATAPECPAWPTVNDNNSNLPRTPPTSSCTTDADCGDISRFACNSFT
                     NQCVCLHNGWRRKTEDDSSRVTGSSVFDFNGDGAAEVIYNDECRFRIYDGLTGHVYFS
                     EPSESRTRIEYPIVADVDNDGNAEIVFSTSTESGFCSEGSNPAIKAQYNAGIEVWGDA
                     NDTWVSARRIWNQHAYHVTNVTESGVVPVFEPENWKQYNGRTYNTYRSNPRSFGVAPD
                     LTVQAIQVSSPDAVCGELSDQIDIAVKIANIGDLRVGPGTVVGFYGEWANGPVMEALN
                     DASGNPLRYIIQTSLEPGTSIIFSVNYQASYHASNVLPDVIKVVVDDTHESRECIETN
                     NEMLANVEAGQPLPDLTIQLGAINPATCMSPQVPTTVRNIGSAPVGDYVVRYYAGDPQ
                     AGGSFLHEVLRPGPLAPAGADSFVEAIPNFPQNLEILIYGVVDPDNLIEECNDANNKD
                     DADNKVRCGGIH"
     assembly_gap    5126..5135
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5361..5993)
                     /locus_tag="PK710_16955"
     CDS             complement(5361..5993)
                     /locus_tag="PK710_16955"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOT11469.1"
                     /translation="MASDKSAFQLRAYVFIDSLQPQLAQFMAKTNRVYDPREYDAALF
                     LEIAPAMEIHTKIDQALKSTRVRLGSLVTERVFGMMQVHHSDQGEVRAAGEAVLTASG
                     LSESDRAPCEILQNKVIRSIEQDHAIAFTGMSAGNMALQGESVLIMEAQPAAYLSIAC
                     NEALKAARVKLIDIRVFGATGRLILSGPEAEIDVAAQAASKALDHINTHH"
BASE COUNT         1269 a         1551 c         1829 g         1392 t
ORIGIN      
        1 gcaggtactt cggggcagta caccgtggag cttcaagtgc aagcaccgaa ggaacgctag
       61 atttcgagag gattcaatgc agccgtgagg ttggaggtgc cgtcgatttt gtgatcaacg
      121 tgacggcaac acgggaagga tttttttgag tcgtgctttt gcgatgggag tatcgccttg
      181 tattcgcacg accaggtcgt aatcggaaga cgtgagcact tcagcggacc agatttcgcc
      241 gggttgcaca tcgctttcgg aaaaaagcac gtggccgtcg atgtcggggg cttgtccggc
      301 gtgacgacct gcgaaaaccc attcatgttc ttcgctgggt ccttcgacca gcacctgcaa
      361 gatctgtcct tttttttgcc ggttgttggc tcgagcgatc gggcgttgca cggacatgag
      421 ttttcgaaat cgacgatagc tttctcgtgg agggactttg tcaggcaggt cgaacgcggc
      481 agtgtcttct tcgtcggaat agcgaaagac acccagatga tcgaagcgtg cccactgaac
      541 gaacgatacg agctggtcga aatcctcttt cgtttcacca ggaaacccaa cgatgaatgc
      601 ggagcgcaag ctggcatgag gggctcgctt tcgaattcgt tccacaatgg cccgcaatcg
      661 tcgttggcca tgaccgcgcc gcattgtttt tagaaccgga tcagcggcgt gttgcatcgg
      721 catgtcgata taaggtgcaa cgtgaggatg gtgggcaatc aattccagca gagcatcgtc
      781 gagttcatcc gggtacagat aaagcaaacg aacccaatgc aagcccggaa cattggcaag
      841 gcgttgcacc aacgtggcga gcgagctggt ggaagataga tcatggccga atcggagggt
      901 gtcttgcgcg acgagattga tttcgatcac gccttgggag acgagatttt cggcttcctg
      961 gacgatatct tcgacgctgc gagagcggtg tggacctttg atacgcggga tggcgcagaa
     1021 agcgcattgg cggctgcatc cgtcggcgat ttttatgtat gcgctcgatc ccactttcga
     1081 aagcatgcga ggggtggagg cggtggacag gaagccttgt ggggagccta cgcgcaatct
     1141 cggtccttga ccgacgatgg catcagcgac gtgcagtaca tcgccggttc caaggagaaa
     1201 gtcgatttcc gggatttggg atgcgagctg ctcggcatac cgttgtgcga ggcaaccggt
     1261 gacgacgagg cgttggcatc gtcctatttg tttgaagcga gagagttgga aaatggtgtc
     1321 gatggattct tttttggcgg gttcgatgaa gccacaggtg ttgacgatga ggacatcggc
     1381 atccgcgggg ttttcgacgg ggacaaggcc gcgggcggtg gcgttaccaa gaatgatctc
     1441 ggcatccacg gtgtttttgg ggcatccgag gctggtaatg tggaaggtga gcatgacggc
     1501 gggaacacca tggtcgttgc tggtaggagg tgcaagatgt gatgcgcaag aaaggcttga
     1561 ctgtatgctg gcactcgggg taacaagccc atccccttcg cgaggatggc ggaactggca
     1621 gacgcgccag attcaggttc tggtggccgc aaggctgtgg gggttcaagt ccccctcctc
     1681 gcactgaaaa tcaatccgtt cgatttcgtt tcaatccaca acgcgagtcg ctgctctgct
     1741 cccgcggaag ttccgaggct tcatttcatc tgggtcattg gcgatccctc gaaatcgcaa
     1801 cccacgaagg aaaccgtatt tcctggccgg tgaccgcaag ctgcgatcat tcgtcgctca
     1861 tgctttgctt ccggttcagg ctcggacgcc gatacgtcgt ttcaagatgc cggatgaaac
     1921 aatccgatcg gcgctctgga cgttgggaga gtggatgagg gatgggaaag aggcgtgatg
     1981 atgcgtgtgg agagtcagtg tattccgcca caccgcactt tgttgtcggc atcatccttg
     2041 ttgttggcgt cgttgcattc ttcgatcaga ttgtcagggt ctacgacgcc atagattagg
     2101 atctcgagat tttgagggaa gttggggatg gcttccacga acgagtctgc accagcggga
     2161 gcaagtggtc ctgggcgaag tacttcatgc aggaacgagc ctcctgcttg tgggtcgcct
     2221 gcgtagtatc tgacaacata gtcaccgaca ggagcggagc cgatgttacg cacggtggtg
     2281 ggaacctgag gagacatgca ggtagcggga ttgatggctc cgagctgaat cgtcaagtca
     2341 ggcaaaggct gtcctgcttc cacgttggca agcatctcgt tattggtttc aatgcattca
     2401 cgcgactcgt gggtgtcgtc gaccacgact ttgatgacat cgggaagaac attggatgcg
     2461 tgataggaag cctggtagtt gacggagaag atgatggacg ttccgggctc caagctcgtt
     2521 tggatgatgt agcggagggg attgccgctg gcatcgttca acgcttccat cacagggccg
     2581 ttggcccatt cgccataaaa gccaacgaca gttccggggc ccacgcgtaa gtccccgata
     2641 ttagctatct ttacggcgat atcgatttgg tcggacaact ctccgcacac cgcatcgggg
     2701 gatgaaactt gaatcgcttg gaccgtcaag tcaggtgcta cgccgaagga tcgcgggttg
     2761 gatcgataag tattgtaggt gcgtccgttg tattgcttcc agttttcagg ttcgaaaacg
     2821 gggacgacgc cgctctccgt cacgttggtg acatggtatg catgttggtt ccagatgcga
     2881 cgagctgaca cccaggtgtc attcgcgtcg ccccacacct cgattccagc attgtattgc
     2941 gctttgatgg cgggattgct tccctcggaa cagaacccgg actcggtgga tgtagagaat
     3001 acgatttccg cgtttccgtc gttgtccaca tccgcaacga ttggatactc gattctcgtc
     3061 cggctttcag agggttcgct gaagtacacg tgaccagtaa gtccatcgta aatgcggaac
     3121 ctgcattcat cgttgtagat gacttctgct gctccatcgc cattgaagtc gaagacactg
     3181 gaacccgtga cgcggcttga atcgtcttct gtttttctac gccaaccgtt atggaggcag
     3241 acgcattggt tggtgaacga gttgcatgcg aatctgctga tatcgccgca gtccgcgtcg
     3301 gttgtgcatg aggaggtggg gggagtccga ggaagattgc tgttgttgtc gttgacggtt
     3361 ggccacgcgg ggcattctgg agcggtggct tggaagtcga tgacgacgta agcggctccc
     3421 agggctgttc cgacttcagg aaagccatct ccatcgaagt catcgacgtt gggggggcca
     3481 ccgccacccc ctgctttcag gtcgagggac agacgcaatt tgccggtcgg accatcaaat
     3541 acgttcagga atcccgcgtt gatggtgatc acctcgggga tgccgtccaa cggattggcc
     3601 gggcccgtgg caagaacgaa atcagctccc atgacatcgg caatcgcaca gaatccatcc
     3661 ttgcgatcgt tgggcagagc ggcggctcca ttgacggtct gcccatccca tacggtgacg
     3721 agttggcctt tgcaccacgc aacttcatcc ggatcagtct cgcttcccgt gcattcgctt
     3781 tgcaacgtga gccccgcagg acccttgggc atgcgatagg ccgtcgatcc ggcgacgacc
     3841 tccaatccct gaccaccgac gatgttcgcg acgcaggaga cgggaccttg cgcattgatt
     3901 cctgcattca gattgccgac gaacttattc gcaaatgcca gcttgccatt ggcatcgtgt
     3961 tgcaacgtcc atacggttcg tcccacaacg atttccgcaa agccatggtt gtcgagattg
     4021 gcgagggtag gggcgggatt cgggccaccg ttcgcgaaag ttgggctttc cgtgatgata
     4081 tggccgcggt tgtcatagat tcgcaccggg gcattcgaac cgttgccttc aagaacggcg
     4141 acaatctcag ggataccgtc gttgtcgaga tctccgaccg ccaggcttgc ggtagaatcg
     4201 atgtcggctt ccgcgcaagt gcatgggacc gaatcgatgg gggctccttc gttccaggtc
     4261 tttgagccgc agcttgcaaa ataatccttt cccttgttgg ggccgccacc atgaatcgct
     4321 cgaagtgtgc cgttggaagt gaaatcacta ttacaaaacg acgtaaatat gatctcagga
     4381 aaatctcgtt cgtcgattag accgtcgccg ttgtcgtcat cgagattggc gacgatggga
     4441 acgtgaacga cttgcacgga ttttgggaag ggactttcct tggcgactgg attggcttgg
     4501 gtcccacccc aggtgatctc attgactggg agcacgtttt caaacggttc gggaggcaag
     4561 gagcaggtgg gcttgatacc cgttcccaca cagattccag cttcacaagt cgtgtattcg
     4621 gggcaatccc agtcatcttc gcaggtacat gctccgagcg tgtcgtcgca ttcgctggtg
     4681 aagtacggag aaccgccgcc gcaggtgtgg cgcacgtatt ccgagccgcc gttgggagcg
     4741 cattcgatga cgtttgcgcc ctggcaatgg cgatctcccg gtgtgcaaat atccggaacg
     4801 caaatcgagc cattgcaata ttgttcgggc aggcaatcga tatcgtcggt gcatgagtta
     4861 tcggcacaat aggcaccggt agccgtttcg acgcagactt cggttggtga gcaatccagg
     4921 gtctcgcaat cgttgggcat gcaggtgggg ctgccgttcg tgctttcgca atgctgatcg
     4981 ttggggcaat aaaacccagc acaaggatcg gtttcgctgc ctcctgctcc tcccgttccg
     5041 cctgtgggaa tgaacgtttc cccttgttcg tggccatcgg tcgatccgct atcggttccg
     5101 gagccaccgg taccggctcc gccctnnnnn nnnnncctgg gtatcggaac catccgtatc
     5161 gcctccacag ctacacccaa cggagagaaa aagcgcgcct gccaccgtga gaaaacagag
     5221 ccgattcata acagggaatc gtaccccatt tcgaacgaga gtggtttgca aaggggggag
     5281 tgagcgacgg cgatggacgg ttcgcgaacc cgcgaaaata tctcaacctg tggatattta
     5341 tatggaatcg gtcgaactgc tcagtgatgg gtgttgatgt ggtcgagagc cttgctcgcg
     5401 gcttgggcgg cgacatcgat ctctgcctcc ggtcccgaca gaatgagtct gcctgtcgct
     5461 ccgaagaccc gaatatcgat gagtttgaca cgagctgcct tgagggcttc gttgcaagcg
     5521 atggataaat aggcggcggg ttgcgcttcc atgatgagca cggactcgcc ctgcaacgcc
     5581 atgttaccgg cggacatacc ggtgaacgcg atggcgtggt cctgctcgat ggaacgaata
     5641 accttgtttt gaaggatttc gcagggggcc cgatcggatt ccgacaaccc tgatgccgtg
     5701 agcaccgctt cgccagcggc gcgaacctcc ccctgatcgg agtggtgaac ctgcatcatg
     5761 ccgaatacgc gttcggttac cagcgatccc aatcgaactc gtgtggattt caacgcctga
     5821 tcgattttgg tgtgaatctc catcgcgggc gcgatctcga gaaatagggc ggcgtcgtat
     5881 tccctcgggt cgtaaacccg attggttttt gccatgaact gcgcaagttg tggctgaagc
     5941 gaatcgatga atacgtaggc tcgaagttga aaagcggatt tatcagaagc cataggatcg
     6001 atactcctcc aacgctcaga cgcgacaatc tacacgaagc ggagccaacc g
//
LOCUS       DAOSOR010000353         6048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13206_length_6048_cov_4.939262, whole genome shotgun sequence.
ACCESSION   DAOSOR010000353 DAOSOR010000000
VERSION     DAOSOR010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13206_length_6048_cov_4.939262"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /locus_tag="PK710_16960"
     CDS             <1..1236
                     /locus_tag="PK710_16960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11470.1"
                     /translation="AAPALSRAVGEAVRSVASEGGGYRVVGAPLETDTPAPQASALVQ
                     RVVSLVAGALGPTSRGCESELSLENAMLRTNGFPALVYVRTQIASGEVRVTVDRYPMA
                     RNIWDKARGGVPGPSAHGFGRARLDAEVRSFLAPIPLVAQTPRKVPMPAAEVVALACG
                     DWGEDGAIALYMMTRRTILQGRIRQGRIVTLKEKAWNDLSSIAPSPWREPLATMVVGV
                     GGLDVGLTDRAMSVRLDPNFSVVGSYEGMPLSGPSGVACAQRRVGSLAAELSPCMTGE
                     SSAPAAPFAFDAYAFERIVDAKGATTDVWAVRNPTDGSVVLRDDRAREHRLLHVGAQL
                     AIADVDLDGEPELLASKNVLNPQLDALVLRTWRANGRVEKRLEVPIVDGIQALTVCPP
                     DGPHRRNMLVATHKELWVL"
     gene            1233..2582
                     /locus_tag="PK710_16965"
     CDS             1233..2582
                     /locus_tag="PK710_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11471.1"
                     /translation="MNRFSTSRRGLLGAMAGAFVGMLAGPARSMGRIPVGGELSFSLP
                     HDTTVLDPYDLFDPLVALIGSAVFEPVYRQDLSGNIYASLAEGMPEREGENTRVRLRK
                     GLVSARGSSLTARDLLASVERARRSAAGAILQEIPEGKVDRSDLGVAIFRQVEPMALA
                     QALSSPLVVLTSLGSTPSRPDGTGAFLAVPGRGGITLERNPHAARGPSFLRRIRITPA
                     PDLKTSLRSFEAQQADIGWLGAGLHQPRHDAIAFDAGAVGWIVLRTGREAGDWDAPGV
                     AQRLVDSIQPARLAHLGLGPFSSSAVTETWGGPACKLLAPASCAHLVEVGATLASILS
                     RSGHEVELSVMGSQEFKNRRQSRAFALMVDVVRPVGPVGVASLVALVAADDPARASSI
                     VKHPPRLSSFDPRVLARTLRLGVVGGLRVAGATAPSVVMVLSKEGAGWDLGSVYRKR"
     gene            2694..4004
                     /gene="fadI"
                     /locus_tag="PK710_16970"
     CDS             2694..4004
                     /gene="fadI"
                     /locus_tag="PK710_16970"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HOT11472.1"
                     /translation="MASKAKSNGADRVAIVSGLRTPFLKQGSGFKSMTTLELSATVVN
                     ELIHRTGVDPKEITGCVFGQVMPSLDYINLAREVVIRTVLDESVTVAHSVTQACVTSV
                     QALTTAIQAIAYGEHDAMITGGADSMDDLPLGVSRKLTVALMGMQKAKTAMDRVRLLS
                     TVSPGDLVPPTPGYSMEPSTGLTMGQHCEMMVKDWGISRAWQDEIAHQSHSKAAKAWA
                     EGFYDDLVMPVVAPPYKEAFRQDNIVRNDSKLEDYAKLRPVFDKKHGTITAANASPLT
                     DGAAAILVMRESKARALGLEPMGYLRSWAYTAVDPMWQLLIAPAFGIPKALAKAGLTL
                     KDIDLVEMHEAFAAQVACTLEALRSRKFCEEHLGLSEPLGELDPERFNVNGGSVAIGH
                     PFGATGARMVMQGLKELKRRQKQFALMTICAGGAMGVAAVLEVA"
     gene            4004..>6048
                     /gene="fadJ"
                     /locus_tag="PK710_16975"
     CDS             4004..>6048
                     /gene="fadJ"
                     /locus_tag="PK710_16975"
                     /EC_number="1.1.1.35"
                     /EC_number="4.2.1.17"
                     /EC_number="5.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HOT11473.1"
                     /translation="MSDNSNKNLSFELRSDGVAVVTFDVPGEAVNTLQASFADEFEAL
                     IAKLDADPSVKAAIIVSGKADTWIAGANIDMLKACKTAADAEALSRIGQRGFLRLEKL
                     EKPVVAAINGSALGGGFELALACHARVLSDDKKTVLAFPEVQLGLLPGSNGLQRLARL
                     IGLQAALDFGLTGKNMRPSKAKKLGVAAEVVRKSALVEVASKLALDLAKKGSLKVKPS
                     KITLDAKGLTSLALERNPVGRKVLFSKAREMTQKKTYGHYPATERILDVLETYADKGF
                     EASSDLEARYFGELVMSETSRQLIGIFNAVTALKKDTGVDDPEIKPRDVHRVGMLGGG
                     LMGGGICYVTMQNANLPIRLKEINLEGLARGYQYVNDIYAERLKKRQITKFEKLRKSA
                     LLSGTIEFEGLRDTDVMIEAVFENLDLKRKVLAQVEEACGPSTIFASNTSSIPITKIA
                     AEAKHPELVVGMHYFSPVHKMPLLEVIVTEKTAPWVTATAVALGKKQGKTVIVVNDGV
                     GFYTSRILGPLKNEAAFILAEGVAIEQIDGAMKRWGWPVGPITLLDEVGIDIAAHVGP
                     IMVEAFGERMTPPDTMTKLVADDRKGRKNAKGFYLYGEAAKKKGKGKHVDTSVYEVLG
                     IHPDKTLPPEEIQMRCSLQFVNEALRCFGDGILRSPRDGDIGAVFGLGFPPFRGGPFR
                     "
BASE COUNT         1146 a         1521 c         2111 g         1270 t
ORIGIN      
        1 gcggcgccgg cgttgtccag ggcggtgggt gaggcggtgc ggagcgtggc ttcggaaggg
       61 gggggctatc gggtggtggg cgcgcctttg gagacggaca ctccggcgcc gcaagcgtcg
      121 gcattggtgc agcgagtggt ttcgctcgtg gccggggccc tgggcccgac ctcaaggggc
      181 tgtgagtcgg aattgtcttt agaaaacgca atgttacgca caaatggctt tccggccctc
      241 gtgtatgtac gcacccaaat cgcctcggga gaggtgcgtg tgacggtgga tcgctatccg
      301 atggcgagaa atatttggga taaggcgcga ggcggtgtcc cggggccgtc ggcccatggg
      361 tttgggcgag cccggttgga tgcggaggta cgatcgttcc ttgctccgat tccgctggtg
      421 gctcaaacgc ctcgaaaagt accgatgcca gcggcggaag tggtggcgtt ggcttgtgga
      481 gattggggcg aagatggggc tatcgcgcta tatatgatga cgcgtcggac gattcttcaa
      541 ggacggattc gacaggggcg catcgtgacg ttgaaagaaa aagcatggaa cgatctttcc
      601 tccatcgcgc cttccccgtg gcgggagccg ttggcaacga tggttgtggg cgtgggcggt
      661 ctcgacgtcg ggctgaccga cagagcgatg tcggtgcgtc tcgatcccaa cttttccgtg
      721 gtgggcagct atgaggggat gcccctttcg gggccgtcgg gggtggcctg cgcgcagcga
      781 agggtgggtt ctttggccgc ggagctttcc ccttgcatga ccggggaatc gtcggctccg
      841 gcggcgccct ttgccttcga cgcgtatgct ttcgagcgta tcgtggatgc gaaaggtgcg
      901 accacggacg tgtgggcggt acgcaatccc accgatggga gcgtggtgct tcgcgacgat
      961 cgggcaaggg agcatcgttt gctgcatgtg ggcgcgcaac tcgcgatagc ggatgtggat
     1021 ctggatgggg agccggagtt attggcaagc aaaaacgttc tgaatccgca actcgatgcg
     1081 ttggtgttgc ggacgtggcg agcgaatgga agggtcgaaa agcggctaga agtaccaatt
     1141 gttgatggaa ttcaagcgct tacggtctgt ccgcccgacg gcccgcatcg tcggaacatg
     1201 ctggtggcca cgcacaagga gttgtgggtg ttgtgaatcg cttttcgacg agtcggcgag
     1261 gcctgttggg ggcgatggcg ggtgccttcg tggggatgct ggctgggcct gcgcgctcga
     1321 tgggccgcat tccggtgggg ggagaactgt cgttttcgct gcctcacgac accaccgtgt
     1381 tggatcctta tgatttattt gatccattgg tagcgttgat cggctcggcg gtcttcgagc
     1441 cggtctatcg tcaggacctt tccggtaaca tctatgcttc tttggcggag gggatgccgg
     1501 agcgggaggg ggaaaatacc cgcgtgcggt tgcgaaaagg gcttgtttcc gcgcgaggat
     1561 cgtcgttgac ggcgagggac ttgctcgctt cggtggagcg agcgcgacgc agcgcggcag
     1621 gggcgatatt gcaagagatt cccgaaggaa aggtggatcg gtcggacctg ggcgtggcga
     1681 tttttcgtca ggtggaaccg atggccttgg ctcaggcgtt gtcgtcgccc ctggtcgtgt
     1741 tgacgtccct ggggtcgacg ccttcgcggc cggacggcac gggagctttt ctggcggtgc
     1801 cggggcgcgg aggaatcacg ctggaacgca atcctcatgc ggctcgcggg ccatcgtttt
     1861 tgcgtcgtat ccgcatcacc ccggcgcccg atctgaagac gtccctgcgt tctttcgagg
     1921 cgcaacaagc ggatattggc tggcttgggg ccggcttgca ccagccccgt cacgacgcga
     1981 tcgccttcga tgcgggggcg gtgggttgga tcgtactgcg cacgggacga gaagcaggcg
     2041 actgggatgc gcctggggtg gcgcagcggc tggtcgattc cattcagccg gcgcgtctcg
     2101 cccatctcgg actcggcccg ttttcgtcgt cggcagtgac ggaaacctgg ggtgggcccg
     2161 cttgtaagct tctggctccg gcctcctgcg cgcatctggt cgaggtaggg gccaccctgg
     2221 cctcgattct gtcgcgctcc ggccacgaag tggagctttc cgtaatggga tcgcaggaat
     2281 tcaaaaatcg taggcaatct cgcgcgtttg ccctgatggt ggatgtggtt cgccccgtgg
     2341 gcccggtggg ggtggcgagt ttggtggccc tcgttgcggc cgacgatccc gcgcgagcca
     2401 gttccatcgt caagcatcct ccccgtcttt cctcgtttga tcccagggta ttggcgcgaa
     2461 cgttgcggct cggcgtggtg ggagggcttc gggtggcagg ggcaaccgca ccctcggtgg
     2521 tgatggtatt gtcgaaggag ggggcggggt gggatttggg gtcggtgtat cggaagcggt
     2581 aggggggaaa actaattgca aggcaccgtt gacacggtgc gtcaccagtt ctagcgtggc
     2641 ataaactgaa tcgccattca gcgcccgaaa gatgggtatc ccaaggaggg ttcatggcaa
     2701 gcaaggcaaa aagcaacggg gcagaccgtg tggctatcgt atccggtctt cgaacgccgt
     2761 tcttgaaaca aggatctggt ttcaagtcga tgacgacgtt ggagttatcg gccacggtgg
     2821 tcaacgagtt gattcaccgg acgggggtgg atccgaaaga gataacgggc tgcgtatttg
     2881 ggcaggtgat gccctcgctg gactacatca acttggcgcg ggaggtggtt atccgcacgg
     2941 tgctcgacga gagcgtgacg gtggctcaca gtgtgacgca agcttgtgtg acgagcgttc
     3001 aggcgttgac gacggcgatc caggcgattg cgtatgggga gcatgatgcg atgatcacgg
     3061 gtggggcgga tagcatggat gatttgccgc tgggggtgag tcgcaagctg acggttgcgt
     3121 taatggggat gcagaaggcg aagacggcga tggatcgggt tcgtctgctt tccacggtat
     3181 cgccggggga tttggtcccg ccgacgcctg ggtattcgat ggagccgagc acggggctga
     3241 cgatgggcca gcattgcgag atgatggtga aagactgggg aatttcgcgt gcttggcagg
     3301 atgagatcgc gcatcaatcc catagcaaag cggccaaggc gtgggcggaa gggttttatg
     3361 acgacctggt gatgccggtg gtggcgcctc cctataaaga ggcgttccgg caggacaata
     3421 tcgttcgcaa cgattcgaag ctggaggatt acgccaagct tcgtcccgtg tttgataaga
     3481 agcacggtac gatcacggcg gccaatgcct cgccgttgac cgatggtgca gcggcgatct
     3541 tggtgatgcg tgagtccaag gcgcgtgcgt tggggctcga gccgatgggg tacttgcgtt
     3601 cctgggctta tacggcggtc gatccgatgt ggcaattgct catcgcgccg gcatttggca
     3661 ttcccaaagc gttggcgaag gcgggattga ctctcaagga tatcgacttg gtcgagatgc
     3721 acgaggcttt cgccgcgcag gtggcttgca cgctcgaagc gcttcgctcc cgcaagttct
     3781 gcgaggagca cctggggttg agcgagcccc tgggcgaact cgatccggag cgtttcaacg
     3841 tcaatggtgg ttccgtggcg attggtcacc cctttggcgc gacgggagcg cgcatggtga
     3901 tgcaggggct caaggaactc aagcgtaggc agaagcagtt tgcgctgatg accatttgcg
     3961 cgggtggtgc gatgggcgta gccgcggtgt tggaggtggc gtgatgagtg acaacagcaa
     4021 caagaacctt tctttcgagc ttcgatcgga cggtgtggcc gtggtcacct tcgatgtccc
     4081 cggagaggcg gtcaacacct tgcaggcatc ttttgccgat gaattcgagg cgttgattgc
     4141 caagctcgat gcggaccctt cggtcaaagc ggccatcatt gtttccggaa aagccgatac
     4201 gtggattgcc ggagcgaaca tcgacatgct caaggcatgc aagaccgcgg cggatgccga
     4261 ggcgctgtca cgcatcgggc aacgggggtt tctacgcctc gagaagcttg aaaaaccagt
     4321 ggttgccgcg atcaacggct ctgccttggg cggaggattc gaactcgcgt tggcatgcca
     4381 cgcgcgcgtc ttatccgacg acaaaaaaac cgtactcgca tttcccgagg tgcaactcgg
     4441 tcttctgccg ggaagcaacg ggttgcaacg tcttgctcgt ttgatcgggc ttcaggccgc
     4501 gttggatttc ggattgaccg gcaaaaacat gcgcccaagc aaagcgaaaa agcttggggt
     4561 ggccgcggag gtcgtccgaa aatccgcgtt ggtggaagtc gcgagcaagc tagcgttgga
     4621 ccttgcgaaa aaaggctcgc tcaaggtcaa gccaagcaaa atcacgctgg acgccaaagg
     4681 gctgacgtct cttgcgctcg aacgcaatcc ggtggggcga aaggtgttgt tcagcaaggc
     4741 gcgagagatg acccaaaaga aaacctatgg gcattatccc gctaccgagc gcatcctcga
     4801 cgtgctcgag acgtatgcgg acaagggatt cgaagcctct tcggacctgg aagcccgcta
     4861 cttcggcgag ctggtgatga gcgaaacctc ccgacagctc atcggcattt tcaatgcggt
     4921 cacggctctc aaaaaagaca ccggtgtgga tgatccggaa atcaagccgc gcgatgtcca
     4981 tcgcgtggga atgttggggg gcggattgat gggcggaggc atctgttacg tcacgatgca
     5041 aaacgccaac ttgcccatcc gtctcaagga gatcaacctc gaaggtcttg cccgaggcta
     5101 tcagtatgtc aatgatatct atgcggaaag attgaagaaa cgccagatta ccaagttcga
     5161 gaagttgcgc aaatccgcac tactttccgg gacgatcgag ttcgaggggt tgcgggatac
     5221 ggacgtgatg atcgaggcgg ttttcgaaaa cctcgatctc aagcgcaagg tccttgcgca
     5281 agtggaggag gcctgtggtc cgagcaccat ttttgcctcc aacacctcgt cgattcccat
     5341 caccaagatc gcggcggaag cgaagcatcc ggagcttgtg gtggggatgc attacttcag
     5401 tccggttcat aagatgccgc ttctcgaggt catcgtgacg gaaaaaaccg caccgtgggt
     5461 gacggccacg gcggtggctc tcggcaagaa gcagggaaaa accgtgattg tggtgaacga
     5521 tggggttggt ttttatacga gtcgcatcct tggaccgctg aagaacgagg cggcattcat
     5581 tcttgcggag ggggtagcca tcgagcagat cgacggtgcg atgaagcgct ggggatggcc
     5641 ggtgggtccg attaccttgc tcgacgaagt tgggatcgac attgcggcgc atgttgggcc
     5701 gatcatggtg gaggcctttg gagagcgcat gaccccgccc gatacgatga cgaagttggt
     5761 agccgatgat cgcaagggca ggaaaaacgc gaaaggcttt tatttgtacg gcgaagccgc
     5821 gaaaaagaag ggcaagggca agcacgtcga tacgagtgtg tacgaggtgt tgggcattca
     5881 tccggacaag acgttgcctc cggaagagat tcagatgcgt tgttcgctgc aattcgtcaa
     5941 cgaagcgttg cgttgttttg gcgacggcat cctgcgcagc ccgagggacg gcgatattgg
     6001 tgcggtattt ggtctcggtt tcccgccctt ccgaggcggc cccttccg
//
LOCUS       DAOSOR010000354         6048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13208_length_6048_cov_3.887535, whole genome shotgun sequence.
ACCESSION   DAOSOR010000354 DAOSOR010000000
VERSION     DAOSOR010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13208_length_6048_cov_3.887535"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="PK710_16980"
     CDS             complement(<1..1024)
                     /locus_tag="PK710_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11474.1"
                     /translation="MLTNRSSRLWLAFIMTSAVLATSSCFVSLDESLIQQGPASDASL
                     DVETGAGDAKSEADTNNPDTDNDVIITPDADADAEPDSPEDVIDEADVSPEADAPVDV
                     TEASDVETDGDNDAAEGGDPFDGALRECYDDTWCPPVGCKKRKCSQGVCTIASEMKED
                     ITSFELPGNLACNQATNRVCVVGVRNYLAALTADGFVIFNTRNPGAVRLETVPNNIGA
                     GYSYLIRSGERLWAVKNSAMDTTEIAWLDLPLDGRSPVSPPSKATVRVPYLSSRLAAP
                     NDAIYLYHYSSGGNTARYAPGLPTNLPSYFANDAQETGAAASSGPRLLLFSVVVDYNP
                     SPIITYQ"
     gene            complement(1024..1977)
                     /locus_tag="PK710_16985"
     CDS             complement(1024..1977)
                     /locus_tag="PK710_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11475.1"
                     /translation="MNSTIFRHLFFALTLLVFIASPMAQADDAAEARQQYNEGKKALD
                     EGRFREAALHFEATTRLRKHGAGPYAAAMAWEKAEQPARAADNFSLALELPGLDAKSK
                     KEAQKHLDALRQSLGAVAVTGPEDLVVHLEDSTELSPPARLHGTPGSHVLIVVRGDIT
                     EKREVELTAGEVVDLDVTEPLPEPEPEPAAPPPPPPLIDEPEPEPVVHVDPSSPKTRK
                     IIGYSAVGAGVVSAGVAVALGFKTLSARDTFEEQRTQQSYDDAKQLRLWTNVAWAGAV
                     VLGGVGTTLLLWPEDKPRKTTEDSSFSLSATPTGVQLRGSF"
     gene            complement(1986..3656)
                     /locus_tag="PK710_16990"
     CDS             complement(1986..3656)
                     /locus_tag="PK710_16990"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT11476.1"
                     /translation="MEAISDNPARPSGALPATSPLGSDALYPGQKLGRYTLLVPVAQG
                     GMARVWAARQSGQRGFQKIVAIKTIMPALAADPEFERMFLDEARIAAAVHHPNVCEIH
                     DLGEVGDVLFLAMEWVDGISLLTLLKARPRTPLEDPVAVRIVADACAGLHAAHELRDD
                     NGYPLNVVHRDVSPHNILLSINGQVKVADFGVAKAMGLEHAPTVAGQLKGKVAYMSPE
                     QAVGDTTVDRRSDIFSMGAVLYEALTGYRAWAGANDVARLQNLLAGNIIPPKQVRPDI
                     PQDLENILAYSLAIDPARRYQTAEQMRMALEKYLVSRSMMVSASNVANSVRMLCGREI
                     EIRQAQIRQAAAAIPDETMGMPGASVEPYSVQYRSRGMRRSTATAITFVAGGITIAGI
                     MGIVAVSSLPSAPAATPPTVASNPPTQTASLAPPPPQNPNIIVVRPSPSDAEIEVNHV
                     ILGKGVRSFDRPEPGQTHTVVVRAPGFETATLTINHDATESEWSVELTPSPDAAASAV
                     SPPATATPTAPPTTVVAPQPPPAATPTTPVVKPTPKTGKTPAIPDNPF"
     gene            3891..5600
                     /locus_tag="PK710_16995"
     CDS             3891..5600
                     /locus_tag="PK710_16995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11477.1"
                     /translation="MINTKCPECSARFFVSEDLVANKTVRFRCRECGGTITVDGRQQE
                     GGGEPNDGTQATRASGAPGGKHATLRPTASAISEFSEKIGDAAGHNEGGDKAERQIDS
                     DSTVKPAPGKSSAAKTPLPTEPKEKPSRPSKPADLDGEPTTEWTKSQAIREKLSPPSS
                     KPRTSRVSMELGSVFAVPQDEEDVEVPVDEDDSAASPDSEDIHLEPIESVPPAPNRLA
                     PPAPPAPKIKPPPPKPKNLDRPSSGRVDIGAALFRSESLSLDSPVDLFAPVAVPASTM
                     TDELPPSEPPAAPLLSPTPAIAVPQATASSDDIKPASSPASSGRGWWVAVAAAVLLAT
                     GGAYFAFGRTTSSEQASLSTSASPPLETQVPALPEPSEKPSDDPAVVKAEPDSPATSA
                     AVPPSSEPEPVAAALPPSGASEPERDSHAMAPAAVTKADSAAATPAPTKTEVAAATPA
                     PTSTPTAAATTKPSTPAAVPTPPPPGEGGGAPFDRAAASASIAALKSAAQSCKQPEGP
                     KGNASVSITFAPSGRVTVATVSGPPFAGTPVGGCIAATFRGASVPPFSGPNVTVRTMV
                     PMF"
     gene            5651..>6048
                     /locus_tag="PK710_17000"
     CDS             5651..>6048
                     /locus_tag="PK710_17000"
                     /inference="COORDINATES: protein motif:HMM:NF024788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HOT11478.1"
                     /translation="MPSEGWDEWLPDILTYSTIAVEILGFALIPWVLIRRKDPGSTIA
                     WILTLLFLPGFGALLFLLFGRSRVRWSAKRKRSADEALRGNLIGFRATLSDVRPLPVP
                     LAGQSAGLFRVGEVLSRSRTTEGNRVDVLEG"
BASE COUNT         1243 a         1658 c         1881 g         1266 t
ORIGIN      
        1 attggtaggt aatgatcggg gacgggttgt aatcgactac caccgagaac agcagcaagc
       61 gagggccgga ggaggctgcg gcacctgttt cttgggcatc gttggcgaaa tacgatggca
      121 ggttcgtggg aagccctggg gcatagcggg cggtgtttcc tccgctagaa taatgataca
      181 ggtagatggc atcattgggg gccgcgagtc gagaagacaa gtagggcaca cgtacggtgg
      241 ctttcgacgg tggagacacg gggctcctac catcgagggg tagatcgagc caggcaatct
      301 cggtggtgtc catggcgcta tttttgacag cccagaggcg ttcaccactt cggatgaggt
      361 aggaataacc agcgccaata ttgttgggaa cggtttcgag tcgtacggcg cccgggttgc
      421 gcgtattgaa gatcacgaaa ccgtccgcgg taagtgctgc cagatagttg cggacgccga
      481 ccacacagac tcgattggta gcctggttgc atgccaggtt tccaggaagc tcgaacgagg
      541 tgatgtcctc tttcatctcg ctggcgatgg tacaaacgcc ctgcgagcat ttgcgttttt
      601 tgcatcccac cggagggcac caagtatcgt cgtaacactc gcgcaacgca ccatcgaagg
      661 ggtcaccccc ttcggcagca tcattatcgc catccgtctc cacatcggag gcctcggtga
      721 catccacggg ggcatcggct tcgggggaaa catcggcttc gtcaatgaca tcctcgggcg
      781 aatcgggttc ggcatccgca tcggcatcgg gtgtgatgat gacatcatta tccgtatccg
      841 gattattggt atccgcttcg gattttgcat ctccggcacc cgtctctaca tcgagactcg
      901 cgtcggaagc agggccttgt tggatgaggg attcgtcgag ggacacgaaa cacgaggaag
      961 tcgccagcac ggcactggtc atgatgaaag cgagccacag acgggaagaa cggttcgtga
     1021 gcatcagaat gaacctcgta gttggacacc ggtgggtgtg gcggacagag agaaagagga
     1081 atcctccgtt gtttttcttg gcttatcttc cggccatagc agcagcgtcg tgccgacgcc
     1141 tccgaggacg acggcgcctg cccacgcaac attggtccat aagcgaagtt gtttggcgtc
     1201 atcgtaggat tgctgggtgc gttgttcctc gaacgtgtcg cgagcggaga gggttttgaa
     1261 gccaagggcc acggcgacac cggcggatac gacgcctgcg ccgaccgccg aataacctat
     1321 aattttccgt gtcttaggtg aggaagggtc gacatggacg acgggttctg gttcgggttc
     1381 atcgatgagc ggaggaggag gtggaggagc tgctggctcg ggttctggtt ctggcagcgg
     1441 ttcggtgaca tcgaggtcga cgacttcgcc tgcggtaagc tccacctctc gtttttcggt
     1501 gatatcgcct cgaaccacga taaggacatg agagcctgga gttccatgca aacgagctgg
     1561 tggtgataat tcagtggagt cttcgaggtg aacgacgagg tcttcagggc cggtgacagc
     1621 cacggctccc agggattgcc ggagcgcatc gagatgtttt tgtgcttctt ttttggactt
     1681 ggcatcgagt ccgggcagtt cgagggcgag ggagaagtta tccgccgcac gagccggttg
     1741 ctcggctttt tcccacgcca tggcggcagc gtaggggcca gcgccatgct tccgcagtcg
     1801 ggtggtagct tcgaagtgca aggccgcttc gcggaatcgt ccctcgtcaa gcgccttttt
     1861 cccttcattg tattgctgac gagcctctgc ggcatcatcc gcttgagcca tgggcgaagc
     1921 gatgaaaacc agcagggtga gagcgaaaaa aagatgacga aagatggttg aattcatggc
     1981 atgaatcaaa aagggttatc ggggattgca ggtgttttgc cagtcttggg cgttggtttg
     2041 acaacgggtg tcgttggcgt cgcagcagga ggtggttggg gggcgacgac agtcgtgggt
     2101 ggagccgttg gtgttgccgt cgcgggggga gaaaccgcgg aagcggcggc atccggcgat
     2161 ggcgtcaact cgacactcca ttcggattcc gtggcatcat gattgatggt gagggtggcg
     2221 gtctcgaaac caggggctcg tacgacaacg gtatgggttt gacctggttc aggacgatcg
     2281 aagcttcgga cacctttgcc gagaatgacg tgattgacct cgatttccgc atcggaaggc
     2341 gatggtctga cgacgatgat attgggattt tgggggggag gtggtgccaa agacgccgtt
     2401 tgcgtgggtg gattcgaagc cacggttggt ggcgtggccg cgggagcgga tggcaaggag
     2461 ctgacggcga cgatacccat gatacccgcg atggtgatgc cccccgccac gaaggtgatg
     2521 gccgtagcgg tggaacgtcg cataccgcgg gagcgatact gcacggaata gggctcgacg
     2581 gaagccccgg gcattcccat ggtttcgtct gggattgcgg cggcggcttg tcgaatttgc
     2641 gcctgacgaa tttcgatttc gcgaccacaa agcatgcgaa cggagttggc cacattgctt
     2701 gcggaaacca tcatgcttcg ggagacgaga tacttttcga gggccattct catctgctcg
     2761 gcggtttggt agcggcgagc aggatcgatc gcgagcgagt aggcgagaat gttttcgagg
     2821 tcttgaggga tatcgggacg cacttgtttg ggcggaatga tgttacctgc caacaaattc
     2881 tgcagtcgcg cgacatcgtt ggctcccgcc caggcacgat atcccgtcag ggcttcgtac
     2941 agcaccgcac ccatcgaaaa aatatcggag cgacggtcta cagtggtatc tcccaccgct
     3001 tgctccggcg acatgtacgc gacttttcct ttgagttgcc cggcaactgt tggcgcgtgt
     3061 tcgagtccca tcgctttggc cactccaaag tccgctactt tgacttggcc gttgatgctc
     3121 agcaaaatgt tgtgaggaga tacatcgcga tgtaccacat tgagaggata gccattgtcg
     3181 tcgcgaagct cgtgcgcggc atgtagcccc gcgcaggcat ccgcaacgat acgtaccgcg
     3241 acgggatcct ccaaaggagt tcgcggtcgt gctttcaaca gtgtgagaag cgatatgcca
     3301 tcgacccatt ccatggcgag aaataggaca tcacccactt cacccaggtc gtgaatctcg
     3361 caaacgttgg gatgatgaac ggcggctgca atgcgagcct cgtcaagaaa catacgttcg
     3421 aattcggggt cagcggcgag tgcgggcatg atcgttttaa tggcgacgat cttttgaaat
     3481 cctcgttgac cggattgacg cgcggcccaa acgcgtgcca tcccgccttg tgccaccggg
     3541 acgagcaaag tgtagcgtcc aagcttctga cctggataga gcgcatcgga gcctaacggg
     3601 gaagtggctg gcagcgcgcc gctcggtcta gctgggttat cggagattgc ttccattcca
     3661 aacctaatct atcaccactg gcggtcttta cccaccgatg agcgattgct attgccatga
     3721 gacgaaaaat cttcgacttg tcgcgtcggt tgcctctggc tccaggatgc gagatgtgcg
     3781 acgctgtcgt atggtggtgc gttcgccgtg aatcgtgagt ccattttggt attatccgat
     3841 gctatgccac ccaagacttc gacctggggg gcaagagggt tggctgggcg atgatcaaca
     3901 cgaagtgtcc cgaatgttct gctcgatttt ttgtatcaga agaccttgtg gcgaacaaaa
     3961 ccgttcggtt ccgatgccgg gagtgcgggg gaacgattac ggtggacggc aggcagcagg
     4021 agggcggggg agaaccgaac gacggcacac aagctacgag agcctctggc gcaccgggag
     4081 ggaaacacgc gaccttgcgt ccgacggcga gcgcgatatc tgaattcagc gaaaaaatag
     4141 gcgatgcggc ggggcacaac gaagggggcg acaaggccga acgtcaaatc gattcggatt
     4201 cgaccgtcaa accggcgcca ggaaaaagca gtgccgcgaa aacgccgctg ccaacggagc
     4261 ccaaggaaaa accatcgagg ccttcgaaac ctgcggacct cgatggtgag ccgaccaccg
     4321 aatggacgaa aagccaagca atacgcgaaa aactcagtcc gccttcctcc aaaccacgga
     4381 cttctcgtgt gtcgatggaa cttggttccg tgtttgccgt gccccaagac gaagaggatg
     4441 tcgaagtacc cgtggacgag gacgattccg ccgcgtcgcc cgattcggag gatatccacc
     4501 tcgagccgat cgaaagtgta ccgccggcgc cgaaccgttt ggctcctcct gctccgcccg
     4561 cgccgaaaat caagcctcca cctcccaaac ccaaaaatct cgatcgtccc agttccggac
     4621 gcgtcgatat tggcgccgca ttatttcgct ccgaaagcct ttctttggat tctcccgtcg
     4681 acctgttcgc gcctgtggcc gtgcccgcga gcaccatgac cgacgagttg ccaccttcag
     4741 aacctcccgc cgctccgctg ctttctccaa cgccagccat cgcggtaccg caagccacgg
     4801 cttcttccga tgatataaaa ccagcttctt cacctgcttc ttcgggacgc ggatggtggg
     4861 tggcggtagc agccgctgtg ctgctcgcga ccggtggcgc ctatttcgcc tttggtagaa
     4921 caacatcttc ggaacaggct tcgctttcga catcggcctc gccgcccctc gagacacaag
     4981 tgccggcgtt gcccgaaccg tcagagaaac cgtcggatga tcccgcggtc gtgaaggcag
     5041 agccggactc tcccgcgact tcggccgcag ttccgccaag ttccgaaccg gaacccgtag
     5101 ctgctgcctt gccgccctct ggagcgtcag aacccgagcg agactcccat gccatggctc
     5161 ccgccgcagt cacgaaagca gactcggcag cagcaacacc agcacctacc aaaacggagg
     5221 ttgcagcggc aacacccgcg cccacgtcca cgccgacagc ggcggccacg acgaagccat
     5281 cgactcccgc cgcggttcca acgccgccgc ctccagggga aggtggcggg gcgccttttg
     5341 acagagcagc cgcttccgcc tcgatagccg ccctaaaatc cgcggcgcaa agctgtaaac
     5401 agccggaggg acccaaagga aatgcttcgg tttccattac cttcgctcct tccggtcgcg
     5461 ttaccgtcgc caccgtcagc ggtccgcctt tcgctgggac tcccgtgggt ggatgcatcg
     5521 ccgccacgtt tcgtggtgct tcggtgccgc cgttttcagg accgaacgtg accgttcgca
     5581 ccatggtgcc catgttctga cccacaccgt gttccgagac agaatggaca attcaggcta
     5641 ggcttgaagc gtgccttcag aagggtggga cgagtggctt cctgatatcc tgacgtattc
     5701 caccattgcg gtggagatac tgggatttgc gctcattccc tgggttttga ttcgtagaaa
     5761 agacccaggc tcgacgatag cctggatcct gacgttgcta tttttgcccg gctttggagc
     5821 gttgttgttc ttgttatttg gccgatcgag ggtgagatgg tccgcgaaac gaaagcgctc
     5881 ggccgatgaa gcccttcgcg gcaacctgat cggttttcgt gcgacgcttt cggatgtgag
     5941 gccgttgccc gttccgcttg caggacagag tgcaggactg tttcgcgtgg gagaggtgct
     6001 atcgcgatct cgaacgaccg aaggcaatcg agtggacgta ttggaggg
//
LOCUS       DAOSOR010000355         6027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13278_length_6027_cov_3.611688, whole genome shotgun sequence.
ACCESSION   DAOSOR010000355 DAOSOR010000000
VERSION     DAOSOR010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13278_length_6027_cov_3.611688"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..1207)
                     /locus_tag="PK710_17005"
     CDS             complement(161..1207)
                     /locus_tag="PK710_17005"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOT11479.1"
                     /translation="MRPPEGFGNIVMPPQTRLDEPRYQGKAGDMTAEQVEYVIQQEDA
                     GERLDVVVVRALRGRAGRARVRDLFESRLVLVQGREATKGWRARPGDVLTIRLASIDP
                     KAVPEPAAALDIRLERSDLVVVNKPAGQPSCPLRPKEIGTLANALVGRYPEMADIGYD
                     PREPGLIHRLDTGTSGLLLAARTPSAFDYLTRDLRAGKIEKHYLAICESDGMADSGII
                     DIPIGIDPKHPKRVVACLRAEELRRYDPRPARTKYSVKQRLGRWALLMVQAPKAVRHQ
                     IRAHFAAIDHPLAGDAQYGGDTRILKRHALHAVSIAWNGRQDVQGFEVSCGMPEDLSA
                     FLQDASIPIPEHDS"
     gene            1377..2687
                     /locus_tag="PK710_17010"
     CDS             1377..2687
                     /locus_tag="PK710_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11480.1"
                     /translation="MIVFLSDLHIEDESVGKHNVSADAFPIFFEHLQRLGKRRSVKEL
                     RIVLLGDTIDLLRTSYWFDVDQEHRPWGWKQDSLAPLAMHTKIVLDKIIDKNRNTFES
                     IRQGVASLRKSIPTNVTFVVGNHDRLANISPVVRRRIRDVLGETPIDDPFEERFVCQR
                     HGVVALHGHQFDVYNHESVSDANRWGVPIGDPITTELVARIPACVLKEIERQSIVLSD
                     EETEQIQKALERIEDVRPVFAVVDWLLSFVRHAPALEQAMYRGLDEALRMFRDLPFVR
                     WWLDSHDTWTKWDDAADRLQWVMWIAGHVSLQTASRLGPAVERMLTALQTADPYVIAA
                     QQLSRQSGAPFVVMGHTHAPKQQALGLQPSQAASPVRRVYLNTGTWRPCHHRCADKSG
                     FIVWKQMSYVIVYSPEERNSEGMPSFETWTGTLDSGESSHFAHH"
     gene            complement(2684..5587)
                     /locus_tag="PK710_17015"
     CDS             complement(2684..5587)
                     /locus_tag="PK710_17015"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT11481.1"
                     /translation="MNPIFKAWVRVFTALFLVTIPFRIYADDLADEADLHFQIGAERY
                     RARDFRGALEHFLLSNRLVANRNVVFNIARTHEQLRQYSHAFRYYQQAREGETNPEEI
                     AKIDAAIDKILPQVAVLHVITDPPGATVYVERKDLGPRGTTPRMLAYEPGDHTLIVEL
                     PGYRSQILESVHLEVGHPKTVHLALEKIVGTIVIGGIPASAEVRTDDPTAPASCQGPC
                     RLEVSAGRHTLYINDPAHESWMREIDVVEGKFVSLRPTLTPQLGSLVVRADEREAIIE
                     IDGKPVGFTPAVIDVGVGLRTVRVSKKGFVPVERTVQIRLKERVRLQVELEAVEEVIA
                     ASRRAEAVEDAPGSVTLVSHRELRAMGYPTIAEAIRGVRGVYVGDDRSYQGVGIRGLF
                     MPGSYGNRVLVLLDGHPMNDNWIGSSYVGYDARTDIEDIDRLEVIRGPGSVLYGTNAF
                     SGVINMVSRARQLPSSGSVGMSSSGSGVARARARATLRLGDDAGFWMSVAGARSEGRD
                     FYFQEYDQPNDVPATDGVVRGLDGFQSGTMTGQAWWGPITWLGFVHSREKDLPTGAYD
                     TIFADPRARQKDTRGMTELRVEPSISDDVHVLLRAHLDMYRFEGRYPYEEVNGGLGVD
                     RYQGTWAGGEGRLILSPWSPLSVTLGGEGQRHFRVHQTAERNDGTHMDRSDPYSVAAG
                     YGSVDVLPMTELRISAGSRFDWYSTFGTSVNPRFAVIVKPYEAGNLKLFAGKAFRAPS
                     IYELYYNDGGRTQVESPDLRPETIYSPELQFTHRITESWSALVTGYANYVQDLIAVQG
                     DATAASPAHYENSPSAILTLGAEASVRREWRHGWMLELSYAYQHSEYRQPSATSVPMR
                     HVPNVPEQMGSIRTSAPIVQGALHAMTRLSVEGPRYDRYDRPGEPEQRRTEGAAIWDV
                     VLSGQEQELGLRYAIGIYNVFDSRHRVPVSREFRQRSILQNGRTLLLSSTVDF"
     gene            complement(5584..>6027)
                     /locus_tag="PK710_17020"
     CDS             complement(5584..>6027)
                     /locus_tag="PK710_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HOT11482.1"
                     /translation="LPISAGALFVLFRHTSTTEASRASDQPGAAEPSTPSHAFVLWID
                     STPSGAEVLEGDTVLGTTPMQLSIDNDTVRDLPKRLTLRAPGHRPYSLVQGPSKDNVR
                     VAVALQPVPAEDSDSAPKPLPRKPSGPSTPPHSPPTADPGIRLHR"
BASE COUNT         1354 a         1743 c         1666 g         1264 t
ORIGIN      
        1 aaccaacacg cgccgcaaga ctccaggcga aagtcataac cctaatcgga tagtcgccga
       61 aggatcgttt caacggaaac gccacagctt gcctcgccca ctgcacggcg acggacgtga
      121 cgccacccca atggacctct cttagagggt aggttcggga tcaggagtca tgctcgggaa
      181 ttgggatcga ggcgtcttga aggaaggctg acaagtcctc cggcatacca caactcacct
      241 cgaagccctg aacgtcttgc cgaccgttcc aagcgatcga aacggcatgt aaggcgtgac
      301 gtttcaaaat ccgtgtatct ccaccgtatt gagcatcgcc cgcgagcgga tggtcgatgg
      361 cggcgaagtg agcgcggatt tggtggcgaa cggctttggg cgcttgcacc atcaaaaggg
      421 cccatcgtcc caaccgttgc ttgaccgaat acttcgttcg cgcgggccga gggtcgtaac
      481 gtcgcaactc ttctgctcgc aggcaggcaa ccacgcgttt gggatgtttc ggatctatcc
      541 cgatcggtat atcgatgatg ccagaatcag ccatcccatc gctttcacaa atggcaagat
      601 agtgcttttc tattttcccc gcgcgaaggt ctctggtcag ataatcgaaa gcggaaggtg
      661 ttcttgcagc gagcaacaaa ccggatgttc ccgtgtccag tcggtgaatg agccccggct
      721 ctctggggtc atatccgatg tcagccattt ccggatacct tccgaccaga gcgttagcca
      781 acgtgccaat ctctttggga cgaaggggac aagaaggttg tcccgcaggc ttgttgacaa
      841 ccaccaaatc agatctttcg aggcgaatgt ccaatgccgc tgccggttcc ggaaccgctt
      901 tgggatcgat ggatgcaaga cggatggtaa gcacgtcccc gggtcttgcg cgccatcctt
      961 tggtggcttc gcgaccctgc acgagtacca atcgagactc gaaaagatct cgaacccgcg
     1021 cccgccccgc tcgaccccgc aacgctcgga cgacgacaac gtcaagccgt tcacctgcat
     1081 cctcttgttg aatgacgtat tcgacttgct cggccgtcat gtcgccagct tttccttgat
     1141 accgaggctc gtcaagcctt gtctgtggcg gcatgacgat gtttccgaag ccctcgggcg
     1201 gtcgcatacc atggaaccta gcggtcggat actccgcggt cctggacgga tggtaggaga
     1261 gccgaaatca tccttcctcg gcaagtacca ccctgatttt ttccaatgat catgccaacg
     1321 cgaccaaaat gcgtatggtt tgcctgtcgt tcctttgggt cctggagcgt gtcgtcatga
     1381 tcgtgttctt gagcgatttg catatcgaag atgaaagcgt aggaaaacac aacgtctccg
     1441 cggatgcttt ccccatcttt ttcgaacatc tgcagcgctt ggggaagcga cgctccgtca
     1501 aggagcttcg catcgtgctg cttggggaca cgattgatct gctgcgaacg tcttattggt
     1561 tcgatgttga ccaagaacac cggccatggg gatggaagca ggattcgctt gcaccccttg
     1621 ccatgcatac gaagatagtg ctcgacaaga tcatcgacaa aaacagaaac acgttcgaat
     1681 ccattcgtca gggtgttgcc tcgctccgaa aatcaattcc aaccaacgtg acgttcgtgg
     1741 ttgggaatca tgaccgactg gccaatattt cgcccgtcgt tcgacgccgt atccgcgatg
     1801 ttcttggaga aaccccgatc gacgatccgt tcgaagaacg tttcgtgtgc caacgacacg
     1861 gtgtcgttgc cctgcatggc caccagttcg atgtgtataa ccatgaatcg gtgagcgacg
     1921 ccaaccgatg gggcgttccg attggtgatc cgattaccac ggaattggtg gcgcggattc
     1981 ccgcatgtgt tttaaaagaa atcgagcggc aaagcattgt cctttccgac gaggagaccg
     2041 agcagattca aaaggctctt gaaaggatcg aagacgttcg ccccgtgttt gcggtggtcg
     2101 attggctatt gagcttcgtg cgccacgcgc ccgccctcga acaggccatg taccggggat
     2161 tggatgaagc gctccgcatg ttccgagatt tgcctttcgt acgctggtgg ctcgattccc
     2221 atgatacgtg gacgaaatgg gatgacgcgg cggaccgttt gcaatgggtg atgtggattg
     2281 ccgggcatgt ctcgcttcaa accgcatccc gactagggcc tgccgtcgaa cgaatgctca
     2341 cggcgttgca aaccgcggac ccgtacgtga tagccgcaca gcaattgtcg aggcaatcag
     2401 gcgcgccgtt cgtggttatg gggcacacgc atgccccgaa acaacaagcg ctcggactgc
     2461 agccctccca agcggcctcc cctgtccgac gcgtgtatct gaacaccgga acgtggcgcc
     2521 cctgccatca tcgctgcgcg gacaaaagcg gattcatcgt ttggaaacaa atgtcatacg
     2581 tgatcgtgta ctcccccgaa gaacgcaaca gcgaaggcat gcccagcttc gaaacgtgga
     2641 caggtacgct cgattcaggg gaatccagcc acttcgctca tcactaaaaa tcaacggtcg
     2701 aagatagaag tagtgttcgt ccgttttgta agatggatcg ctgacgaaac tcacggctga
     2761 caggcacccg atgacgggaa tcgaagacgt tgtagatgcc tatcgcgtaa cgaaggccga
     2821 gttcttgctc ttgcccggat agcacgacat cccagatggc cgctccttcg gtcctgcgtt
     2881 gttctggttc tccaggtcgg tcgtaacgat cgtagcgcgg cccctctacc gacaatcgag
     2941 tcatggcatg aagagcacct tgcacaatcg gagccgaagt gcgaatcgag cccatttgct
     3001 cgggcacgtt ggggacatgg cgcataggga ccgacgtcgc cgatggctga cgatactccg
     3061 aatgctgata cgcataggat aactcgagca tccacccatg acgccattct cgccgcacgg
     3121 aagcttctgc accaagtgtg agaatcgctg agggggagtt ttcgtaatgg gcgggagagg
     3181 cagcggtggc atctccttgt accgcgatga gatcctggac gtaatttgcg tatccagtca
     3241 ccagggccga ccatgattcg gtgattcgat gagtgaattg aagctccggc gagtagatgg
     3301 tttccggacg aagatcggga ctctcgacct gcgttcgtcc cccgtcgttg taataaagct
     3361 cgtaaatgga gggtgcccga aacgctttgc ctgcaaagag tttcaaattc cccgcttcat
     3421 agggtttgac gatcaccgcg aaccgaggat ttaccgacgt gccaaacgtc gaataccagt
     3481 cgaaccgaga gcccgccgat attcgcagtt cagtcatggg aagcacatcc accgacccat
     3541 agccagcagc aaccgaatac ggatcggaac gatccatgtg ggttccgtcg ttgcgctcgg
     3601 ccgtttggtg gactcgaaag tggcgctgac cctcgcctcc caacgtcaca ctaagcgggg
     3661 accacggcga aaggatgagc cttccttctc ctcccgccca cgtgccttga taacgatcga
     3721 cccccaaccc accgttgacc tcttcgtacg gataccgtcc ttcgaagcga tacatatcga
     3781 gatgggcgcg cagcagcaca tgaacgtcgt cggaaatcga cggctccaca cgcaattccg
     3841 tcatgccgcg ggtgtctttt tgacgagcac gaggatcggc aaaaatggtg tcataggctc
     3901 ctgtcggtaa gtctttttcc cgcgaatgaa cgaagcccag ccacgtgatc ggtccccacc
     3961 acgcttgacc tgtcatcgtt cccgactgaa agccgtccag accacgaacg accccatcgg
     4021 ttgctggcac atcattgggc tggtcgtact cttgaaaata aaaatcccta ccttccgaac
     4081 gcgcccccgc caccgacatc caaaaccccg catcatctcc caatcgaaga gttgctctcg
     4141 ctcgcgctct ggccacaccc gatccactgc tggacatccc tacgcttccg gaagatggca
     4201 actgccttgc gcgcgacacc atattgatga cacccgaaaa cgcattggtg ccatacaaca
     4261 ccgaacccgg tccacgaatc acctcgagac gatcgatgtc ctcgatgtcc gtacgcgcgt
     4321 cgtagccgac ataggaagaa ccgatccaat tgtcattcat aggatggccg tccaagagaa
     4381 cgagcacgcg gttgccgtag cttccaggca tgaacaatcc acgaatgcct actccttgat
     4441 aggagcgatc atctcccaca tacacccctc gtacaccacg aatggcctcc gcaatcgttg
     4501 gatagcccat cgcacgaagc tcccgatggg aaacaagagt caccgacccc ggcgcgtctt
     4561 cgaccgcttc cgcgcgtcga gatgccgcga tgacttcctc gacggcttca agttcgacct
     4621 gcaatcgaac acgctccttc aatcgaatct gaaccgtgcg ctcgacaggt acaaacccct
     4681 tcttgctcac ccgaaccgtt cgtaacccaa caccgacatc gatgacagcc ggtgtgaatc
     4741 caaccggttt gccatcaatt tcgatgatgg cctcgcgttc gtcggcacga acgaccaacg
     4801 agccaagctg cggagtcaag gtgggacgaa gcgagacgaa cttgccttcc acgacatcga
     4861 tctcacgcat ccatgattca tgagccgggt cgttgatgta caaagtatgt cgacccgcgg
     4921 atacttcgag tcggcaaggt ccttggcatg aggcaggtgc ggtaggatca tccgttcgaa
     4981 cctccgctga cgcgggaatg cctccgataa cgatggtgcc aacgattttt tccaaggcga
     5041 gatggacggt tttggggtgc ccgacttcga gatgaaccga ttcgaggatc tgggaacgat
     5101 aacccggtag ttccacgatg agcgtgtgat ctcccggttc ataagccaac atgcgaggag
     5161 tcgtgccacg aggaccaagg tcttttcgct ccacatagac ggtggcacca ggaggatcgg
     5221 tgatgacgtg aagtacggct acctgcggaa ggatcttgtc gatggccgca tcgatcttgg
     5281 cgatttcttc tgggttggtt tctccctccc gcgcttgttg gtagtaccga aaggcgtgcg
     5341 aatactgacg aagctgctcg tgggttcgcg cgatgttgaa gacgacattg cggttggcca
     5401 cgagacgatt cgagagcaag aagtgctcga gagcgccccg gaagtcgcgg gcacgatacc
     5461 gttccgcccc gatttgaaag tgcaaatccg cctcgtcagc caaatcgtcg gcgtatatcc
     5521 gaaaagggat agtcaccagg aatagcgccg tgaatacacg gacccatgct ttgaaaatag
     5581 gattcatcgg tggagtcgaa tgccgggatc ggcggtcggt ggggaatgag gaggcgtcga
     5641 ggggcccgac ggttttctag gaagtggctt gggagcacta tcgctatcct ctgcaggcac
     5701 tggctggagc gcgacggcca cgcgaacatt gtccttcgat gggccttgta cgagcgaata
     5761 cggacgatgc cctggggccc gaagcgtcaa gcgcttgggc aaatcacgaa ccgtatcgtt
     5821 gtcgatcgac aattgcatgg gcgtcgttcc aagtaccgta tcaccttcca acacctcggc
     5881 tcccgaagga gtcgaatcga tccacaacac aaacgcatgg gaaggtgtcg acggctcggc
     5941 ggcccccggt tggtcggacg cgcgggatgc ctcggttgtg ctcgtgtgtc gaaacagcac
     6001 aaatagtgct cccgccgaga ttggcag
//
LOCUS       DAOSOR010000356         6025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13284_length_6025_cov_4.718928, whole genome shotgun sequence.
ACCESSION   DAOSOR010000356 DAOSOR010000000
VERSION     DAOSOR010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13284_length_6025_cov_4.718928"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1425)
                     /locus_tag="PK710_17025"
     CDS             complement(<1..1425)
                     /locus_tag="PK710_17025"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HOT11483.1"
                     /translation="MTTLPPVGATDALYIVDLSGYVFRAYHAIAPLSSPTGEPSHATY
                     GTITMLNRLIDERKPTLMAVAMDSKGPSFRKQIDNAYKANRPPPPEDLSIQMQRVRGL
                     VSLLGLTVWKQDGFEADDLIASGVKRACSEGLRVVIISADKDLMQLVGASVVMWDTMR
                     NKVYGPAEVREKFGVFPDKMRDLLAMTGDSSDNVMGIPSVGPKTAAKLLDQYGSIEGV
                     YEGIDEIKGKLRENLVEHRASAELARLLVTLRDDLDVSFERSALDFHARTDWKGLEAA
                     YVELGFSRMLEAMRPQMEIDFDQPQADDYRLVLEEADLRSAVQACHEAGLMGLDTETD
                     STDAMRARLVGISLSCRPHEGWYIPVGHAYLGVPSQLDIDRVREILNPLLISSEVRKV
                     GHHVKFDETVLRRHGFGPMQAVASDTMIESYLLDPEASHGLKEVAKRELHVEMMTYEQ
                     VVGGKGSKAVTFDKVPLERASRYAC"
     gene            1521..2882
                     /locus_tag="PK710_17030"
     CDS             1521..2882
                     /locus_tag="PK710_17030"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT11484.1"
                     /translation="MNDAADAPNSEWIAGKYRLIRMIGQGGMGTVWEGQHITLGTKVA
                     IKMIEHDLAENHEARNRFDKEARAAATLSSRYVVTVFDHGVMPDGRPFIVMEYLRGQT
                     LEERAATNHRLPLVEVARIIDQVARGLSKAHEHGIVHRDLKPENIFLTESPDEDETVA
                     KIVDFGIAKFIEGTPLSQPSSTRSGTILGTPVYMSPEQARGLRDIDHRSDLWALGVIA
                     YRCAVGTLPFQGEAVGDIVVNICTKDPVLPSTLVPSLPKAFDDWFRRCVARDREMRFD
                     SAKELASSLWAASGSSPPQSFATDLLNPHLVESRTNASHSSIESATTQVATTNPPGRS
                     RMSRGVLGGTLVLLGACASLLVGWWYSSASLSEPMAAEASHPYFPEEIQIVNMIAPAA
                     NSEEEASISLPESMASNAAASDAPSSSRPKIRPPQASAVQTASRGPSRTLPPSTTTID
                     LGY"
     gene            2985..3281
                     /locus_tag="PK710_17035"
     CDS             2985..3281
                     /locus_tag="PK710_17035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11485.1"
                     /translation="MTDTRILWDFDVRVRDRNLKTGRLKKDEVDRYYDNLVDVTNNVD
                     AMEARQPTLPTAEDKHEEEPSAVQGSPSMGYAPAAPGLPMMQPIIPHAKNEDDY"
     gene            3307..3594
                     /locus_tag="PK710_17040"
     CDS             3307..3594
                     /locus_tag="PK710_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOT11486.1"
                     /translation="MADEKAQQATELPKTDFSTFVLSLSHSALVHLGDAPSPDGVART
                     PNLVLARQTIDLLEILSEKTKGNLTGEEERLLEHILYELRMRFVEVAKEKS"
     gene            3591..4772
                     /locus_tag="PK710_17045"
     CDS             3591..4772
                     /locus_tag="PK710_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOT11487.1"
                     /translation="MNGWMRAWRAASLTISLLTLLLPVSCGKGHSSPTAEQTESAVPA
                     VQAASVTPPTTAAAPTPTMMPMGAPMSFASLAKTADPGVVTIQSRVERTFGSGRRRVL
                     SEGLGTGFVYDKAGLVLTNNHVIEGATAVVVQFASGRSVQAEVKGRDRHTDVAVLQLE
                     KAEGLHALPLGDSDGIEVGDWVVAIGNPFGLAHTVSAGILSGKGRTKDDVKGLDPSGY
                     FNFLQTDAAINQGNSGGPLLNLRGEVVGINTAIRANANNIGFAIPINMVKELLPILVK
                     DGKITRSALGISVRELDSTESERLKRPDTKGAWVVDIAPGSGADRAGIRPDDVILGFD
                     GKQVLDPNMLRWWASIAGVGKTVDVKVGRGTRTFDVKVTLGELPEQPEPPSPGFPPGF
                     P"
     gene            4772..5530
                     /locus_tag="PK710_17050"
     CDS             4772..5530
                     /locus_tag="PK710_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT11488.1"
                     /translation="MDDNIRHEEGSAGSVGGEQATTVVNTQSVVASNREQRRAEAEED
                     RVLIEAAQKGDVAAFRALVEKHQRRVFSIALGIVRDENDAREISQEAFIRVYRGLDRF
                     QGGSSFFTWLYRIVTNLSIDLIRRPGRRDAELVEDRKVYDVADGSLEPFVARLEGEDP
                     LRVVRRREIAERLQEALDALPPYHLGVILMREVEGMSYEEMAEAMGVSKGTIMSRLFH
                     ARQKLQRALRDCYEEEIESIDEPMSASGKHEAQP"
     gene            5527..>6025
                     /locus_tag="PK710_17055"
     CDS             5527..>6025
                     /locus_tag="PK710_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11489.1"
                     /translation="MNNRANNSDGRGELSSDSVTSHDLMMFADGELDAQREAAVASYL
                     RENETGRAVVSTVWEMGARIRELQDRKAIDAGADGIASLVMARIEQEASAPRKENVVP
                     LVTSERRAVGFGPVVVGGLALAAGVALLVWRLVGMDLTQPIPEKTAGALSSEKPVSVS
                     ALSTED"
BASE COUNT         1339 a         1635 c         1831 g         1220 t
ORIGIN      
        1 gcacgcataa cgagacgctc gttcgagtgg caccttatcg aatgtgactg ccttcgaacc
       61 tttgcctccc accacctgct cgtaggtcat catctccacg tgaagttcgc gtttcgcaac
      121 ctccttcagt ccatgggaag cttcagggtc caacagataa ctctcgatca tcgtatcgga
      181 ggccacggct tgcatcggac cgaagccgtg gcggcgcaac acggtttcgt cgaacttcac
      241 atggtgcccc accttgcgaa cttcactcga aatcagcagt ggattgagaa tttcccgaac
      301 gcggtcgatg tccaattgcg aaggaacgcc aaggtaagca tgcccgaccg ggatgtacca
      361 cccctcatgg ggacgacagg atagagagat tcccacgagg cgagctcgca tggcgtcggt
      421 ggaatccgtt tccgtatcca ggcccatcag gccagcctca tgacaggctt gcacggccga
      481 acgaagatcc gcctcctcaa gcaccaagcg ataatcgtct gcctggggct gatcgaagtc
      541 gatctccatt tgtggacgca tcgcttcgag catgcgcgag aacccaagct cgacatacgc
      601 ggcctccaag cctttccaat ccgttcgagc atgaaaatcc aaggccgatc gctcgaacga
      661 aacgtccaag tcgtcgcgca aggtcacgag caaacgtgca agttccgcag aggcgcgatg
      721 ctcgacaagg ttctcccgaa gcttgccttt gatctcgtcg attccttcgt acaccccttc
      781 gatggatccg tattgatcca gcagcttggc cgccgttttc ggtccgaccg agggaatgcc
      841 catcacattg tcggaactat ctcccgtcat ggcgagaaga tccctcattt tatcaggaaa
      901 aaccccaaat ttttctcgca cttccgcggg cccgtacacc ttgttgcgca tcgtgtccca
      961 cataaccaca gatgcaccaa ccaactgcat gaggtccttg tccgcgctga tgatcaccac
     1021 gcgaagacct tccgagcagg ctcgtttcac cccactggcg atcaagtcat ccgcttcgaa
     1081 accatcttgt ttccagacgg taagaccaag caaggacacg agcccgcgaa cgcgttgcat
     1141 ttgaatcgaa aggtcttcgg gaggaggcgg gcgattcgct ttgtaggcat tgtcgatttg
     1201 ctttcgaaac gacggacctt tcgaatccat ggccaccgcc atcagcgtcg gctttcgctc
     1261 atcgatcagt cggttgagca tcgtgatagt gccgtaggtg gcatgcgacg gctcgccggt
     1321 cggagaggaa agcggagcaa tcgcgtggta tgcgcgaaac acgtagccag acagatcaac
     1381 gatatacagg gcatcggtgg ccccaaccgg aggaagcgtc gtcatgggag ccatgcgtag
     1441 cgcacattga gcaaggatag aatgcttacg gttggcgacc gcaccaagga aaaggtagtc
     1501 taggggcggc gaacgaaacg atgaacgacg cagcagacgc tccgaacagc gaatggatag
     1561 ccggcaagta ccgattgata cgcatgatcg gacaaggggg aatgggcacg gtctgggagg
     1621 ggcaacacat tacgttggga acgaaggtcg cgatcaagat gatcgaacac gacctggccg
     1681 aaaaccacga agcacggaat cgattcgaca aggaagcccg cgccgcggca acgctttcct
     1741 ctcgttatgt cgtcaccgtc ttcgaccatg gagtgatgcc cgatggtcgt cctttcatcg
     1801 tcatggaata ccttcggggg caaacgctcg aagagcgtgc cgctaccaat caccgtctgc
     1861 ctcttgtcga agtggcacgg atcatcgacc aggtcgctcg cggcctgtcc aaagcgcacg
     1921 agcatggcat cgtgcatcgt gatctcaaac cagagaatat cttcctgacc gagtctcccg
     1981 atgaagacga aaccgtggcg aagatcgtcg atttcggcat tgccaaattc atcgaaggca
     2041 ccccgctgtc tcagccatcg tcgacgcgtt ccggtacgat tttaggaacg cccgtgtata
     2101 tgagtccaga acaggctcgc ggccttcggg atatcgacca taggagcgac ctttgggccc
     2161 tgggcgtcat cgcttaccgc tgcgccgtcg gcaccctacc gttccaaggc gaagccgtcg
     2221 gcgatatcgt tgtcaatatc tgcaccaaag atcccgtgct ccccagcaca ctcgttcctt
     2281 cccttcctaa ggctttcgac gattggttcc gtcgttgcgt ggcccgggac cgcgagatgc
     2341 gctttgactc tgccaaagaa ctcgcctcat cgttatgggc ggcgtcgggt tcatcgcccc
     2401 ctcagtcctt cgccaccgac cttctcaacc cgcacctggt cgaaagccgc acaaatgctt
     2461 ctcattcatc gatcgagagt gccacgacac aagtcgcgac cacgaatccg ccagggcgca
     2521 gccgaatgtc caggggtgtg ttgggaggaa cactcgtgct gttgggagct tgcgcatcgc
     2581 tgctggtggg ctggtggtat tcgtcggctt ccctttccga accgatggcc gccgaggcgt
     2641 cccatcctta ttttcctgag gaaatacaaa tagttaacat gattgcgccc gcagcgaact
     2701 cggaagagga agcctcaatt tccttgcctg aatcgatggc ctcgaatgct gccgcctcgg
     2761 atgcgccatc gtcatcacgc ccaaaaatac gcccacccca ggcatcggct gtccaaaccg
     2821 caagccgggg accaagtcga acgttaccgc cctcgacgac aaccattgac ctagggtatt
     2881 gagactgcaa acctgccaag cgacgctccg cctgttgacc aatgcctcgc aaagatccag
     2941 ggtcatacgg ctcagcccaa ggcgagtacc atgatggaga aatgatgacg gacactagaa
     3001 ttttatggga cttcgatgtg cgcgttcgag accggaactt gaaaaccggt cgtctgaaga
     3061 aagacgaagt ggataggtac tacgacaacc tcgtggacgt gaccaacaac gtcgatgcga
     3121 tggaggcgcg tcaacccaca ttgccgaccg cggaagacaa gcacgaggaa gagccttcgg
     3181 cagtccaagg gtcaccgtcg atgggatacg cccccgcggc tcccggacta ccgatgatgc
     3241 agcctatcat tcctcacgcc aagaacgagg acgattactg agttacgggc cctatgggag
     3301 gcgagcatgg cggacgaaaa ggcacaacaa gccactgaac ttccaaagac ggatttttcc
     3361 acgttcgtgt tgtcgttgag tcattccgcg ctcgtgcatc tcggggacgc gccatccccc
     3421 gacggggttg cgcgaacgcc caatctcgtt ttggcccgac aaaccattga tttgctcgag
     3481 attctttcgg aaaaaaccaa gggcaacctc acgggagaag aagaacgtct gctcgagcat
     3541 attttatatg aactgcggat gcgtttcgtc gaggtcgcca aggagaagtc gtgaacggct
     3601 ggatgcgcgc ttggagggca gcgtcgctga cgatcagttt gctcacgctg cttttgccgg
     3661 tatcgtgtgg caaagggcat tcttcgccga cggcggagca gacggaaagc gcggttcctg
     3721 ctgtccaggc ggcatccgtg acgcccccca cgaccgcggc ggcgccgacg ccgaccatga
     3781 tgccgatggg ggcacccatg tcgtttgcat ccttggcaaa aaccgcagat cccggcgtgg
     3841 tgacgatcca aagccgtgta gaacgaacgt ttggaagcgg tcgacgtcgc gtgctttccg
     3901 aaggacttgg aacgggattc gtgtacgaca aagccggtct cgtgctcacc aacaaccatg
     3961 tgattgaggg ggccacggcg gtggtggttc aattcgcgag cgggcgttcg gtgcaagcgg
     4021 aggtgaaggg acgggaccgt catacggacg tggcggtttt gcagttggag aaagccgaag
     4081 ggctacatgc gttgccgctg ggtgactccg atgggattga ggtaggtgat tgggtggtag
     4141 cgattggcaa tccttttggg ctggctcaca ccgtttcggc ggggatattg tctggaaaag
     4201 ggcgaacgaa ggacgatgtc aagggcctgg acccgagcgg gtatttcaat tttcttcaga
     4261 cggatgcggc gatcaaccag ggcaattccg gcgggccttt gttgaatttg cgtggggaag
     4321 tggtgggaat caacacggcc atccgcgcga atgccaacaa catcggtttc gcgattccga
     4381 tcaacatggt caaagagctg ctgccgattt tggtcaagga tgggaagatc acgcgtagcg
     4441 cgctgggtat cagtgtccgg gagttggatt ccaccgagtc ggaacgattg aaaaggccgg
     4501 acacgaaggg agcgtgggtg gtggatattg ctccgggctc cggagccgac cgcgcgggaa
     4561 ttcgtccaga tgacgtaatt ttaggatttg acggtaaaca agttctggat ccgaatatgc
     4621 ttcggtggtg ggcgagcatt gcgggtgtgg gcaagacggt ggatgtgaag gtgggaagag
     4681 gaacgaggac gttcgatgta aaagttacct taggggagtt accggaacaa ccggaaccac
     4741 cgtctccggg atttccgcct ggatttccgt gatggacgat aatatccgcc atgaggaagg
     4801 ctctgctggg agcgtaggag gggagcaagc tacgaccgtg gtgaataccc aatcggtggt
     4861 tgcgtccaac cgtgagcaac gaagggcgga agccgaggaa gatcgtgtac tcatcgaagc
     4921 agcgcagaaa ggtgacgtgg ccgcatttcg agccctggtg gaaaagcacc agcggcgagt
     4981 attctcgatt gcccttggca tcgtgcgtga cgaaaacgac gcgcgagaaa tttctcaaga
     5041 agcctttatt cgggtgtacc gcggattgga ccggttccag ggcgggtcga gcttttttac
     5101 ctggctttat cgaattgtca ccaacttatc cattgacctc attcgtcggc ccggcaggcg
     5161 cgacgcggag cttgtggaag atcgaaaggt gtatgacgtc gcggatggga gtctcgaacc
     5221 tttcgtggcc cgcctggagg gggaagaccc tctgcgagtc gtgcgtcgcc gagaaattgc
     5281 cgaacgtttg caggaggcgc ttgacgcatt gccgccctac cacttgggcg tcattctgat
     5341 gagagaggtt gaaggcatgt cgtacgaaga aatggccgag gcaatgggcg tgtccaaggg
     5401 cacgatcatg agccgtctat ttcacgcacg acagaaactc cagcgagcac ttcgggattg
     5461 ctatgaagag gaaatcgaat ccatcgacga gccgatgagc gcgtcgggaa agcatgaggc
     5521 tcaaccgtga acaaccgcgc caacaatagc gatggccgtg gggaactgtc ttcggatagc
     5581 gtgacttccc acgatttgat gatgttcgcg gacggggagt tggatgcgca gcgtgaggct
     5641 gccgtagcct cgtatctccg ggagaatgag actggccgtg cggttgtgtc cacggtgtgg
     5701 gagatggggg cgaggatacg ggaactgcag gatcgcaagg cgatcgacgc cggtgcggac
     5761 gggattgctt ctttggtgat ggctcgcatc gagcaggagg cgtctgcacc gcggaaagaa
     5821 aatgtcgtcc cgttggtaac gtcggagcgt cgtgccgtgg ggtttggccc tgtggtggtg
     5881 gggggattgg cgttggcggc tggggtggcg ttgttggtgt ggcggctggt ggggatggat
     5941 ttgacacagc ctatccctga gaaaacggcc ggggctttga gcagtgagaa gccggtatcg
     6001 gtttccgcct tgtcgacgga agacg
//
LOCUS       DAOSOR010000357         6023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13294_length_6023_cov_4.627513, whole genome shotgun sequence.
ACCESSION   DAOSOR010000357 DAOSOR010000000
VERSION     DAOSOR010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13294_length_6023_cov_4.627513"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PK710_17060"
     CDS             complement(<1..450)
                     /locus_tag="PK710_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11490.1"
                     /translation="MQRHSWIVTFDLGNVVVRLSGSTRESAKRAGIPWRPATHAERPE
                     QASHLLERWQLGQIDDDRLFEAWADALGGRFSAAEAEIISQAWLLGEFEGIHDIIQRL
                     DQRHVRLGCLSNTCNHHWQWMLNHPDRFPSFALLHEKHASHLMGVMKP"
     gene            708..1067
                     /gene="ndhC"
                     /locus_tag="PK710_17065"
     CDS             708..1067
                     /gene="ndhC"
                     /locus_tag="PK710_17065"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOT11491.1"
                     /translation="MVETYAPLLLMFALAFVVVIGFFLASHYLGPKKPSPEKMMPFES
                     GNPSEGASHLRMSVKYFLTAISFVVFDVEVVFLYLWAVHFKSLGGAGLLMAAAFLAML
                     LIGLVYELRKGALEWEN"
     gene            1055..1630
                     /gene="nuoB"
                     /locus_tag="PK710_17070"
     CDS             1055..1630
                     /gene="nuoB"
                     /locus_tag="PK710_17070"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HOT11492.1"
                     /translation="MGELTKPTSSRVIQAGESGFATTKFNELLAWARKYSLFQYPFVT
                     ACCAMEFMATASPRYDVARFGAEAPRFTPRQSDVLWVVGTISQRQAPILKRVYEQMTE
                     PKWVIAFGTCASCGGFYDNYATLAGIDKVVPVDVYIPGCPPRPEAVLDGLLLLQDKIA
                     SMDTTPGVVKPRHDPAQNLVNLRRKTEDSAG"
     gene            1645..2229
                     /locus_tag="PK710_17075"
     CDS             1645..2229
                     /locus_tag="PK710_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOT11493.1"
                     /translation="MAKKLLDILSERFGSSIVLSTHDQCGDETAVLDAAHWREVATFL
                     RDDPRCKMNMLVDLCGVDHIWDSQRLEVVAHLNSLELGHRLRIKARVGDEDCEGAVID
                     SLVPVWVGANWFERETFDMFGIRFEGHPDLRRILLYEEFEGHPLRKDYPAAKTQPLVP
                     YREGPDVLGKLPPFGPDEGMVFGRKHLDYMKEED"
     gene            2229..3440
                     /locus_tag="PK710_17080"
     CDS             2229..3440
                     /locus_tag="PK710_17080"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOT11494.1"
                     /translation="MEPLDQVEDNALELPSDPMQLMMGPSHPAMHGTVRIAVELSGEV
                     VVKADVQIGYLHRGFEKSCERGTWAQVFPYVDRCNYSSPMLNNVGFALAVEKLSGMQV
                     PERCQYYRVILGELARMADHLVCNAAMAMELGAFTPFLWWTRAREMIYDLFEEETGAR
                     VTHSFCRIGGMAYPPTDTFQEHVLAILDKVLAASDEGISLLETNRIFMDRTMGVARIS
                     PEEAIAFGWTGPCLRSTGVPYDIRKAHPYLVYDRFDFDVPVGHEGDNYDRLMVRAEEI
                     HQSARIIRQAVEQMPKDGPINLYDASAVLPPKPQVYTTIEATIHHFKMIMEGLKVPKG
                     EAYGYTEAANGELGFYVISDGSGTPYRVRIRPPSFAIVGGLGKLITGQMVPDIIPTFG
                     SMNMIGGECDH"
     gene            3469..5118
                     /locus_tag="PK710_17085"
     CDS             3469..5118
                     /locus_tag="PK710_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOT11495.1"
                     /translation="MPTFKIDGKDVPFEAGDTIMRAAYRAGIDIPYYCWHPGLSVAAN
                     CRMCLVEILPPPGRPPMLLDVLEFDRTTGQYVNTRKPKLQPACQVPVTENMEVLSDTS
                     EHVKRARHAVQEFLLLNHPVDCPICDQAGECKLQDYWLDHMRTPKRMPDELIHKPKAV
                     SFGPTIVYDAERCIICTRCVRFCEEVAKDPVLSVRERGNLNEITVSPGRELDHPYTFM
                     TELVCPVGALTTKDFRFKARVWFLRSAPSICQGCATGCNAWLDFDPRTQRAYRYRPRD
                     NESVNSYWMCDEGMLSYHATHDARATQIRVGGKHTLAAKAIEKAVELIKNAPKDKSAV
                     VLSAQHNCEDNFALVTLARAMGIQAWFLIGKPEGQGDDILRHVDKNPNTAGARAVAER
                     ELKGIEELTRAVEDGSIKHLIVMGSVWPADADSLLDKLTSLVVIGGWQGALHEHAEVF
                     LPASSWAETHGIYVNAKGMAQESRRAYPGADDCQPGWKFVREVCVGLGHEPAWNSLSS
                     LREAMPKACLPKPEINVSASADHHANANETAADKAAASTDS"
     gene            5129..>6023
                     /locus_tag="PK710_17090"
     CDS             5129..>6023
                     /locus_tag="PK710_17090"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOT11496.1"
                     /translation="MTIVELIFTLLKIAFILVFCLAFAGALTWNDRRMSSMMQDRIGP
                     NRARFFLPPTLARGMFAGPALLAAAGIGAWGWLTSFPDTVAGALRMTERILLTAELSV
                     FITWTVLAMLTTWAVRRGSSNALEAFLAEQVRDPRRVFYLGLVAHIAVFVLRYALRGT
                     EWAASIKPALFLAAPSVLALLIAACGLLAASKVPKEGFKLRVIGLLHTMADGIKMIWK
                     EDFMPKRGDRLLHSIGPMLGMFPPLVVMAVVPFGDVLCFAKNAEGGIQWTQILPVVSR
                     DGVCAEAGIPMQIANLDIGILF"
BASE COUNT         1260 a         1703 c         1735 g         1325 t
ORIGIN      
        1 cggtttcatg acccccatca agtggctagc atgcttttcg tgaagcaagg cgaacgatgg
       61 aaaacgatcg ggatggttaa gcatccattg ccagtggtgg ttgcaggtat tggacaggca
      121 tccgagccgt acgtggcgct gatcgagacg ttggatgata tcgtgaatac cctcgaattc
      181 accgagcaac caagcttgtg aaatgatttc ggcttccgcg gcggagaagc gacctcccaa
      241 ggcgtcggcc caggcttcga acaggcgatc gtcatcgatt tggcccaatt gccagcgttc
      301 caaaaggtgc gaggcttgtt ccggccgctc cgcgtgggtg gcgggtcgcc acggaattcc
      361 cgcgcgtttt gctgattcac gggtcgaccc actcaggcgc acgactacgt tgccaaggtc
      421 aaacgtgacg atccatgaat ggcgctgcat ggtttgccgg atatcgacag gggcggaatg
      481 tacaacagcc gtcgagaatc accaatggtg cgatggaagg ccatgccaca gattgggcga
      541 aaatgggatg gtggtcgttg gtattggctt gaaatcatga gccatcctgt ccggcggtct
      601 gttcaattga cagggttcga gcccccgact acgtaacccc atcgcacgat accgcgcact
      661 tcggctcggt atcgtgggtt cgtacctcct tcgaggagcg tgtcttcatg gttgagacct
      721 acgcaccgct cttgctgatg tttgccctcg cgttcgtcgt ggtcatcggc tttttcttgg
      781 cgagccacta tctggggccg aagaagccgt ctccggagaa gatgatgccc ttcgagtctg
      841 gcaatccttc ggaaggggcc agtcatttgc gcatgagcgt gaagtacttc ctgaccgcca
      901 tctcctttgt ggtgttcgat gtggaggtgg tgtttctcta cctctgggcg gtacacttca
      961 agtccttggg cggggcgggg cttctgatgg ccgcggcgtt tctagccatg ttgctgatcg
     1021 ggctggtgta cgaattgagg aaaggagcgc ttgaatggga gaactgacga agccgacctc
     1081 gtcgcgggtc attcaggcgg gtgagtctgg gtttgccacg accaagttca atgaattgtt
     1141 ggcgtgggcg cgaaagtact ctctattcca gtatccattc gtcaccgctt gttgcgcgat
     1201 ggagttcatg gccaccgcaa gcccccgcta cgatgtggct cgatttggcg cggaagcgcc
     1261 tcggtttacc ccgcgtcaaa gcgatgtgct ctgggtcgtg ggaaccatca gtcagcggca
     1321 agcgcccatc ttgaaacgcg tttacgaaca aatgacggaa cccaagtggg tgatcgcctt
     1381 tggtacctgc gcgtcgtgcg gtggttttta cgacaattac gcaacattgg cgggcatcga
     1441 caaagtcgtg ccggtcgatg tgtacatccc tggatgtccg ccgcgaccgg aagcggtgct
     1501 cgacggactg cttttgttac aagacaagat cgcgagcatg gataccacgc ccggcgtggt
     1561 caaaccacgt cacgatcccg ctcaaaatct ggtgaatctg cgtcgaaaaa ctgaggattc
     1621 agcaggatag cccggagtac ggtgatggcg aaaaagcttc tcgacattct ctccgagcgc
     1681 tttggtagtt cgattgtatt gagcacccac gaccaatgcg gcgatgagac cgcggtgctc
     1741 gatgcggcac attggcgcga ggtagcgact tttcttcgtg acgatccacg atgcaaaatg
     1801 aacatgctgg tggatctttg cggggtcgat catatatggg actcgcagcg actcgaggtg
     1861 gtggcccatc tcaactcgtt ggaacttggg catcgcttgc ggatcaaggc tcgcgtggga
     1921 gatgaagact gcgagggagc cgtgatcgac agcctggtgc ccgtttgggt cggagccaac
     1981 tggttcgagc gggaaacgtt cgatatgttc ggtatccgct tcgaaggtca tccggatctt
     2041 cgccgcatct tgctttacga agagtttgag gggcatccgc ttcggaagga ttatcccgcc
     2101 gccaaaacac aaccgctggt tccctatcgg gaaggtccgg acgtgttggg aaagcttccg
     2161 ccctttggac cggatgaggg gatggttttc ggacgaaaac acctcgatta tatgaaagaa
     2221 gaggactgat ggaaccgctc gaccaagtgg aagacaacgc tctcgagcta ccatccgacc
     2281 ccatgcaact gatgatgggt ccttcccatc ctgccatgca cgggacggtt cgcatcgccg
     2341 tggagctgtc cggagaagtc gtggtcaagg ccgatgtgca aatcggctat ctgcatcgtg
     2401 gattcgagaa aagctgtgag cgcggaacgt gggctcaggt tttcccttac gtagaccgat
     2461 gcaattactc ctcaccgatg ctcaacaacg tcggattcgc tctcgccgtc gaaaagctca
     2521 gcggaatgca ggttcccgaa cgttgccagt attaccgagt catcctcggc gagttggcgc
     2581 gtatggccga tcatctcgtt tgcaacgcgg ccatggccat ggaactcggc gcatttacgc
     2641 cgtttctgtg gtggacccgc gctcgcgaga tgatttacga cctgttcgaa gaagaaaccg
     2701 gagcgcgcgt cacccattcc ttctgccgca tcggcggtat ggcctatccg cctacggata
     2761 ccttccaaga acacgtgctc gccatcctcg acaaagtgct tgccgccagc gatgaaggga
     2821 tttcgttgct cgaaaccaat cgcatcttca tggatcgaac catgggagtc gcgcgcatca
     2881 gccccgagga ggccatcgct ttcggatgga ccggaccttg cctgcgctcg acgggggtgc
     2941 cgtacgacat ccgaaaagcc cacccgtatc tcgtctatga ccggttcgat ttcgatgtgc
     3001 ccgtgggcca cgagggagac aactacgatc ggttgatggt tcgtgccgaa gagattcacc
     3061 aaagcgctcg gatcattcgt caagcggtcg agcagatgcc gaaagacgga ccgatcaacc
     3121 tatacgatgc ttcagccgtt ttgcctccca aaccgcaagt atacaccacc atcgaagcaa
     3181 cgattcacca cttcaaaatg atcatggagg ggttgaaagt cccgaaaggt gaagcgtatg
     3241 gctatacgga agcggccaat ggtgagcttg gtttttacgt gatctcggat ggaagcggca
     3301 ctccgtaccg cgttcgaata cgtccacctt ccttcgccat tgttggtggg ctgggcaaat
     3361 tgatcactgg gcagatggtt ccggatatca ttccaacctt cggctcgatg aatatgatcg
     3421 gaggcgagtg cgaccactga gcgatccgtg gacgctgggg aattacgcat gccgaccttc
     3481 aagatcgatg gcaaagacgt cccgttcgaa gctggtgaca ccatcatgcg tgccgcctat
     3541 cgcgcgggta tcgatatccc gtattactgc tggcacccag gactgagtgt cgccgctaac
     3601 tgccgaatgt gcttggtgga aatccttcca ccgcccggca gaccacccat gctcctggat
     3661 gtcctcgaat tcgaccgaac caccgggcag tacgtcaata cacgaaaacc caaactgcag
     3721 cccgcgtgcc aggtgcccgt caccgaaaat atggaagtgc tcagcgacac cagcgaacac
     3781 gtcaaacgcg cgcgacacgc cgtccaagaa ttcttgctgc tcaatcaccc cgtcgattgc
     3841 cccatatgtg accaagccgg tgaatgcaaa ctgcaagatt attggctcga ccatatgcgc
     3901 acccccaagc gcatgcccga tgaattgatc cacaaaccca aggccgtctc cttcggtcct
     3961 accatcgtct acgatgccga acgatgcatc atttgcacga gatgcgtccg cttctgcgaa
     4021 gaagtagcca aagacccggt cctgagcgtg agagaacgag gcaacctcaa cgaaatcacc
     4081 gtctctccag ggcgagaact cgatcatccc tatacgttca tgacggaact tgtttgtcct
     4141 gtcggcgcgt tgaccaccaa ggatttccgc ttcaaagctc gcgtctggtt tttgcgctcc
     4201 gcaccgtcga tctgccaggg atgcgccaca ggctgcaatg catggctcga cttcgacccg
     4261 cgtacacaac gcgcctaccg ctatcgtccg cgggacaatg agagcgtcaa cagctattgg
     4321 atgtgcgatg aaggcatgct cagctatcac gccacccacg acgcgcgcgc aactcagatc
     4381 cgtgtcggcg gcaaacacac actcgctgcc aaggcgatcg aaaaagcagt cgaactgatc
     4441 aaaaatgcgc ccaaagacaa atcggccgtc gtgttgagcg cgcaacataa ttgcgaagat
     4501 aacttcgcgc tcgtcacgct cgcccgcgcc atgggaattc aagcttggtt cctcatcgga
     4561 aagcctgaag ggcagggcga tgacatcttg cgtcatgtcg ataagaaccc caacacggca
     4621 ggggctcgcg cggtcgcgga acgggaactc aagggtatcg aagaactgac ccgggccgtg
     4681 gaagacggtt ccatcaagca tctgatcgtg atgggaagcg tctggcccgc cgacgccgat
     4741 tcgctcctcg acaaacttac gtctcttgtg gtgatcggtg gttggcaggg cgctcttcac
     4801 gagcatgcgg aggttttttt gcccgctagc tcatgggcgg aaacccacgg catttatgtc
     4861 aatgccaagg gcatggccca ggagagtcga cgcgcttatc ctggagccga cgattgccag
     4921 ccaggttgga agttcgttcg tgaagtatgc gtagggttgg gacacgaacc ggcctggaat
     4981 tccctttctt cgctgcggga ggcaatgccc aaagcctgcc tgcccaaacc tgaaataaac
     5041 gtctcggctt cggccgatca tcacgcgaat gccaacgaga cagccgccga taaggctgct
     5101 gcctccacgg attcctgagg gcgagagcat gaccatcgtc gagctgatct tcacgctcct
     5161 taagatcgcg ttcatcctgg tgttctgcct ggccttcgcc ggggcattga cctggaatga
     5221 ccgtcgaatg agttcgatga tgcaagaccg catcggaccc aatcgagcgc gattctttct
     5281 tccgccaacc cttgcccgag gcatgtttgc gggtcctgcc ctgctggccg ccgctggtat
     5341 cggtgcctgg ggctggctta cgtcgtttcc cgataccgtc gctggtgcgc ttcggatgac
     5401 ggaacgcatc ttgctcaccg cggagctttc cgttttcatc acctggaccg tactcgcgat
     5461 gctgacgacc tgggccgttc gtcgcggctc cagcaatgct ttggaagcgt ttcttgctga
     5521 gcaagtgcgc gaccctcgac gtgtcttcta tctcggattg gtcgctcata tcgcggtatt
     5581 cgtgcttcgg tatgccctgc gcggcaccga atgggcggct tcgatcaagc ctgcgttgtt
     5641 tcttgcagcg ccctcggtgt tggcgttgct catcgccgct tgcggcctgc tagccgcttc
     5701 gaaagtcccg aaggaaggct tcaagcttcg ggtgatcggc ttgcttcaca ccatggccga
     5761 cggcatcaaa atgatttgga aggaggactt catgcccaaa cggggcgacc gcctccttca
     5821 ttcgattggg cccatgctcg gtatgttccc accgttggtc gtcatggcgg tcgtaccttt
     5881 tggcgatgta ttgtgctttg ccaaaaacgc ggaaggcggc atccagtgga cacaaattct
     5941 gcctgtggta tcgcgcgacg gagtttgcgc cgaggcagga atccctatgc aaatcgctaa
     6001 cttagacatc ggcatcctct tca
//
LOCUS       DAOSOR010000358         6021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13303_length_6021_cov_4.009051, whole genome shotgun sequence.
ACCESSION   DAOSOR010000358 DAOSOR010000000
VERSION     DAOSOR010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13303_length_6021_cov_4.009051"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PK710_17095"
     CDS             complement(<1..258)
                     /locus_tag="PK710_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11497.1"
                     /translation="MKTFTPVVRMLFLGAWMGTRVPVGCLVNTETDSSRFEPTQGEDA
                     SPGDVADASGDAVDDGNEGQGHDGSGHDVANDHAADGPGGRT"
     gene            complement(325..1494)
                     /locus_tag="PK710_17100"
     CDS             complement(325..1494)
                     /locus_tag="PK710_17100"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOT11498.1"
                     /translation="MHNVRHCPELDRLAGWCVHEIQASASAVVACAFRREHGWQVDEG
                     FARIDDPNQPEAGTDTLFDLASLTKSFVALAFARLVRDGLVDWLMPLGVPLRDAHGTQ
                     VASVPLLWLVAHRAGLEGHRPLYAPLQEGKIVDRCLALRQAAMALRPECLAITPKEDG
                     FPPCYSDLGYLLFGEAMSRASGLPLDEIIRAQISQPLGIQARSARQWREGEPAFVQRT
                     APTEVVAWRGGLVRGAVHDENAWALGGDGLCGHAGLFATARDVAMLGAAILDALQGTR
                     DDWLTVEQLEPLVRRRPESTLRAGFDGKNDHGSSSAGERCSPDTFGHLGFTGTSFWID
                     PHAQVVAVLLTNRVYPKRDNLTIQMARPTIHDELFAWGLRHAHRDGRKDADARRM"
     gene            1647..3773
                     /locus_tag="PK710_17105"
     CDS             1647..3773
                     /locus_tag="PK710_17105"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOT11499.1"
                     /translation="MRRSLTADKRWLAWLWLWGMALLTGCYSLAPGRQAVDRIDIQEN
                     TQLRDDEIKGKIATGSSPRFLGIWDGVAFEYAYFDRFVLQQDLARIERYYRSKGYYHS
                     RVRAARVIPTGKQGHVRVEIAVHEGPPTIVRSALPSGLNTVPFEHQAAILNAVEKGLK
                     PGDRFDETIYEQTKDRIRAELKNRGFAWAEVEGRVQVNLLDLSATIQYEIKAGPLFLV
                     RSVTFLGIQDLPREPIRRVFNVNPGDSFSAKDLETGKNALLDLGVLADVQVSWPSGDP
                     QAPTNIPRNKAGQPAVDVVVRCTPAPLRTIKLGVGTELDTIRADVHGVAGWESRNFLG
                     GLRRFTVTAMPGIVFFPTKVGQFESPDRLLPEGKLLASLRQPGFLEPRSTGVLRGNAS
                     IFPVLLQSYSDDVILGYRELKGAVGIERMFWNHHLHVGTFFHGQAYYPFTYVGILDDS
                     LRRVLVRYVELSSYLDFRDDSLRPHRGAFLSNSLQLAGGILGGSVSDVKVQPEVRLYA
                     PISSTVTFAVRSTVGFLFPRDYGQALKDQFDEGDTRDQQLLYFRAFYSGGPSSNRGYP
                     YRAVGPHGPGAFLAPNVSAREFRDRCGAGYSEDACRIPLGGLTLWEASAEVRYPILGP
                     LTGAVFADASDVTRDKTTFHFEYLHLSTGMGFRYDTPVGPIRLDVGYRVPGMQKIGGD
                     LDREERDPGQIFGQPIAFSLAVGQAF"
     gene            3860..5824
                     /locus_tag="PK710_17110"
     CDS             3860..5824
                     /locus_tag="PK710_17110"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOT11500.1"
                     /translation="MASIGCSRSRIVLALVFLPLAFCLGGCGSEDAGFDDARGGSGGS
                     AGSTGSSGSSGSSGSSGSAGSSGSGGSGASSGSSGSSGSGGGETELSAPEIVAPADQT
                     VMEDETLRLSIEVISSAKQTRIWLDGLPPGARFDEDSRQLTFTPDFIQGGNSYPVTVH
                     ASNEAGSTTASFSITIIDSITPSFPTITATSTESGYSKLTLSQTTDAYLDSPGHAGRK
                     FVARVYVPNGATASERMPVRIYLHGFGGAPYNGTSSGGQFRIYPHDSMNSYWWGYGDG
                     SPGGSSTTVPNYTQRRILHLLEWVLRTYPGADPERVYVTGGSMGGAGASAFGLLYGRH
                     FAFVEATIGQMIPRNHRPARIKQLEELWGKVQDNWQDGTSLEDGTALGVWDRQDITRV
                     LLDMPEARDQFLFTKHGKDDATIHFGAVTHASALTGLSYYQTVQKEGIGHIAVWDEGG
                     HGSADPVMGEGWWDDGFSLLFDERSYLRRDRAFPAFSRASHDRDPGDGTGNGKRAWND
                     NSGYAGQVGTAGDTGWSGEIAGALNRFLRWDTNGVVDEWERFEIPLFVVDGKGSAAPK
                     AGYPTRGDRFDGSWPVTVDVTARRIRRFRCLAGEPIRWQWEGKTGTVLANDDGSVTVP
                     QIELDTNVRTLVLTRATGSSTGTAAWEGNP"
BASE COUNT         1220 a         1558 c         1947 g         1296 t
ORIGIN      
        1 agtccggcca ccggggccat ccgcggcatg atcgttggcg acatcgtgcc cggaaccatc
       61 gtgcccctgt ccctcgtttc catcatcgac ggcatcgccg ctggcatcgg caacatcgcc
      121 aggggaagca tcctctcctt gggttggctc gaaacggctg ctatccgttt ccgtattaac
      181 aagacaaccc accggtacgc gggtacccat ccacgctcca aggaaaagca tccgcacgac
      241 aggtgtgaac gttttcatgg ccctggggca aggttagcag aaagctcgga atggctccat
      301 cttcacaata ttttgttgaa aaatttacat tcggcgggcg tccgcgtctt tccggccatc
      361 gcgatgggca tggcgcaatc cccaagcgaa tagttcgtcg tggatggtgg gtcgtgccat
      421 ttgaatcgtc aaattatccc ttttcggata gacacgattg gtgagcagaa ccgcgacgac
      481 ttgcgcatgc gggtcgatcc agaacgaggt tccggtaaat ccgaggtgac cgaatgtgtc
      541 agggctacag cgttcgcccg cggagctaga gccatgatcg ttttttccgt cgaatcccgc
      601 gcgcaaggtg ctttcgggcc gacggcgaac gagcggttcg agttgttcga ccgtgagcca
      661 atcgtcgcgc gtgccttgca aagcatcgag gatggccgcg ccgagcatgg ccacatccct
      721 ggccgtcgca aagagcccag catgaccgca tagtccatcg ccaccaaggg cccaggcgtt
      781 ttcgtcatga acggcgccgc gcaccagccc tcccctccag gctacgactt cggtgggtgc
      841 ggttcgctga acgaaagcgg gctccccctc gcgccattgt cgggcggaac gagcttggat
      901 accaaggggt tgtgatattt gcgcacggat gatttcgtcg aggggcaatc ccgacgctcg
      961 gctcatggct tctccgaaga gcagataccc gagatcgcta taacatgggg gaaatccatc
     1021 ttctttcggg gtgatagcga ggcattccgg gcgaagagcc atggcggctt gccgcagggc
     1081 gaggcatcga tccacgattt ttccctcttg aagcggcgcg tacaaggggc ggtgcccttc
     1141 caatcccgca cgatgggcca ccaaccacaa cagcggaacg ctggcgacct gcgtaccatg
     1201 cgcatcgcgc aacggaacgc ccagcggcat gagccaatcc acaagaccat cacgaacgag
     1261 ccttgcgaac gcgagagcca cgaagctttt ggtcaaagag gcaagatcga agagggtatc
     1321 ggttcccgcc tccggctgat tcgggtcatc gatgcgggcg aaaccttcat ccacttgcca
     1381 tccatgctca cggcgaaacg cgcaagccac cacggcgctt gccgaggcct ggatttcgtg
     1441 gacgcaccaa ccagcgagcc ggtcgagttc tgggcaatgt cgtacgttat gcatcgcgac
     1501 tggtcgtacg ttcatggggg aacaattgca acgggtcgcg gcattgcgga aaccaacggc
     1561 aaagggtatc tgtctaggct agacctcctg ggccgagcct ggaatggtgg ccgcacgaga
     1621 acgtggttgt ctcgacaaca tcgatcatga gacgaagcct gacagcagac aagaggtggt
     1681 tggcttggct ttggctgtgg ggcatggctt tgctcaccgg gtgttattcg ctcgcgccgg
     1741 ggcgtcaggc ggtcgatcgg atcgacattc aagaaaacac gcaactgcgt gatgacgaga
     1801 tcaaagggaa aatcgcgaca ggatcgagtc cgcgttttct tggaatctgg gatggggtag
     1861 cgtttgagta cgcgtatttc gaccgtttcg tgttgcagca ggatctggct cgcatcgagc
     1921 ggtattatcg ttccaaaggc tattaccatt cgcgagtgcg cgcagcgcgc gtcattccca
     1981 cgggtaaaca agggcatgtg cgggtcgaga tcgcggtcca tgagggtccc cccaccatcg
     2041 tgcgttccgc tttgccttcg gggttgaata ccgtgccgtt cgagcatcag gccgccattc
     2101 tcaacgcggt ggaaaaaggg ctgaaacctg gcgatcgatt cgatgagacg atctatgagc
     2161 agacgaaaga ccggattcgt gcggagctga aaaaccgtgg atttgcctgg gctgaggtcg
     2221 aggggcgggt gcaggtgaac cttctcgatt tgagtgcgac gattcaatat gagatcaagg
     2281 cgggaccgtt atttctcgtt cgttcggtga catttctcgg gattcaggat ctgccacggg
     2341 agccgattcg acgcgtattc aacgtgaatc ctggggatag tttttcggcc aaggatctcg
     2401 aaacggggaa aaacgcgctt ctggatctcg gggtattggc ggacgtgcag gtttcctggc
     2461 cttcgggcga tccgcaagcg cccacgaaca tcccgcgaaa caaggcaggg caaccggcgg
     2521 ttgacgtggt ggtccgatgc acccccgccc cgcttcgtac gatcaagttg ggagtgggaa
     2581 cggaacttga tacgattcgg gccgacgttc acggcgtggc tgggtgggaa agccgcaatt
     2641 ttttgggagg attacgacga ttcaccgtaa cggccatgcc cgggatcgtg tttttcccca
     2701 cgaaagttgg gcagttcgag tcccccgacc gattgttgcc ggaaggcaaa ctgctcgcat
     2761 cgcttcgaca accagggttc ctcgaaccgc ggtcgacagg ggttttacgt ggaaatgcaa
     2821 gtattttccc agtgcttctg cagagctatt cggatgacgt cattctcggc tatcgagaac
     2881 tcaaaggcgc ggtgggaatc gaacgtatgt tttggaacca tcaccttcat gtgggaacct
     2941 tctttcacgg gcaggcctac tatcctttca cctatgtagg aattctcgac gatagcctgc
     3001 gcagggtgct ggttcgctac gtggagctat cgtcctattt ggactttcga gatgattcgt
     3061 tgcgtcccca tcgaggagcc ttcctgagca actcgttaca actggcgggg ggaatattgg
     3121 gaggcagcgt atcggacgtg aaagtgcagc cggaagtgcg tttatatgcc ccgatttcct
     3181 caacggtcac gttcgccgtt cgttcgacgg tgggtttttt atttcctcgc gattatgggc
     3241 aagcgctcaa agaccagttc gacgagggcg atacgcggga ccagcagctc ttgtacttcc
     3301 gtgcgtttta ctcgggcggt ccttcctcga atcgtggcta tccgtaccgc gcggtagggc
     3361 cccacggtcc cggagcgttt ttggctccga acgtttcggc cagggaattt cgcgatcgat
     3421 gtggtgccgg gtattcggag gacgcttgtc gtattccgtt ggggggcctt acgttatggg
     3481 aggcatccgc ggaggttcgg tatcccattc tgggaccctt gacgggggcg gtttttgctg
     3541 acgcctccga cgtcacgaga gataaaacta cttttcattt cgaatactta catttatcca
     3601 cggggatggg cttccgctac gatacgcctg tgggacccat tcgtctcgat gtcggatatc
     3661 gcgttcctgg catgcaaaaa atcggcggag atctcgaccg cgaggagcga gatccaggac
     3721 aaatcttcgg ccaaccgatt gctttttctc tggcggtcgg acaggcgttc taacgacttg
     3781 ccccgtgcgt ccgacgactt gctttccgag gggcatcggg acctgggcat ccggggctgg
     3841 ttatcggtag actaggagca tggcgagcat cggatgttca cggtcacgca tcgtattggc
     3901 tttggttttc cttcccttgg ccttttgcct ggggggttgt ggctcggagg atgcgggctt
     3961 cgacgatgcg cgcggcggaa gcggtggaag cgccggcagc actggaagca gcggcagcag
     4021 cggcagtagt ggaagcagcg gcagcgctgg cagcagcggt agtgggggaa gtggcgctag
     4081 cagcggcagc agtggaagca gcggtagtgg tggtggggaa acggagcttt ccgctccgga
     4141 gattgtcgcg cccgcggatc agaccgtgat ggaggatgaa accttacggc tatcgatcga
     4201 ggtcatttcc tcggcaaaac agacacggat atggctggac gggcttccgc ccggagcgag
     4261 attcgatgag gattcgcgac aactcacgtt cacaccggac ttcattcagg gaggcaatag
     4321 ctatccggtt acggttcacg catcgaatga ggccggatcg actaccgcat cgttttccat
     4381 cacgattata gacagtatca caccatcgtt tccaacgatc acggctacat ccaccgaatc
     4441 gggatactcg aagctgacac tatcccaaac gacggatgct tacctggata gtcctggaca
     4501 tgcggggcgc aagtttgttg cacgagtgta cgtccccaat ggtgcgactg cctcggagcg
     4561 catgccggtt cggatctatc tccacggttt cggaggcgcc ccgtacaatg ggacgagttc
     4621 tggaggccag ttccgcatct acccacacga ttcgatgaat tcgtattggt ggggatatgg
     4681 ggatggctct ccggggggaa gttcaacgac agtaccgaat tatacgcaac gtcggatcct
     4741 tcatttgttg gaatgggtgc ttcgtacgta tcctggtgcg gacccggagc gggtctatgt
     4801 gacaggcggg tccatgggtg gggcgggagc gagcgccttt gggctgctct acggtcgtca
     4861 ttttgcgttt gtcgaagcca cgatcgggca aatgatccct cgcaaccatc gtcccgcgcg
     4921 gatcaagcaa ctcgaagaac tctggggaaa ggtgcaggac aattggcaag atggtacctc
     4981 gttggaggat gggacggcgt tgggggtatg ggatcggcag gatatcactc gtgttttgtt
     5041 ggacatgccg gaggccaggg atcaattctt gtttacgaag catggcaagg acgatgcgac
     5101 gatccacttt ggcgcggtca cccatgccag cgcactgacg gggttatcgt attatcagac
     5161 ggtgcaaaag gaggggatcg gtcatatcgc ggtatgggat gagggaggtc atggttcggc
     5221 ggatccggtg atgggtgagg gctggtggga tgatggattc agtctactgt tcgacgaacg
     5281 gagttatctt cgaagggatc gggcatttcc cgcgttttcg cgagcgagcc acgaccgcga
     5341 tccaggcgat gggacgggca atggcaagcg agcgtggaac gacaacagtg ggtatgcggg
     5401 gcaggtgggt acggccggag atacgggctg gtctggtgag atagcgggag cgctcaatcg
     5461 atttttacgc tgggatacga acggtgtggt ggatgaatgg gagcggtttg agattccgct
     5521 ctttgtggtg gatgggaaag gcagcgcggc gccgaaggcg ggctatccta cgcgggggga
     5581 tcgatttgac ggctcatggc cggtgacggt ggacgttacg gcacgacgga tacgtcgttt
     5641 ccgctgtctt gcgggagagc cgattcgatg gcaatgggag gggaaaacgg ggacggtatt
     5701 ggcgaatgac gacggttcgg tgacggtgcc tcagatcgag ttggacacga acgtacgaac
     5761 cttggtgctg acacgggcga cgggttcatc gacgggaact gcggcgtggg aaggcaatcc
     5821 ttgaccgagg gaaccgtcgg tctattctcg ggtgacgttg gcatgctcga cgggttgcag
     5881 taggacagga tgatgaacaa gccttctgga acgggacgga cgggagcggt gcaagatccg
     5941 gagttcgaga cggtccagtt gaaagcggcg gtgccatcgc ggtgggaggt accgagctat
     6001 cgtctcacgg tgatggaggg g
//
LOCUS       DAOSOR010000359         6018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13310_length_6018_cov_4.634245, whole genome shotgun sequence.
ACCESSION   DAOSOR010000359 DAOSOR010000000
VERSION     DAOSOR010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13310_length_6018_cov_4.634245"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PK710_17115"
     CDS             <1..335
                     /locus_tag="PK710_17115"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOT11501.1"
                     /translation="LLPGVSRSGSTIAGGLLGGLRPSLAVSYSFLVGLPLIAAAAAKD
                     TLDGGFGRLLTEVGVLPISVAFVAALVSGLGSIAALKLVVGKRRLVWFGGYCALVAGV
                     CFWMGFSQ"
     gene            complement(368..2923)
                     /gene="acnB"
                     /locus_tag="PK710_17120"
     CDS             complement(368..2923)
                     /gene="acnB"
                     /locus_tag="PK710_17120"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF006690.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HOT11502.1"
                     /translation="MLSEYRIHVQERARLGIPPLPLRADQARVVCDLLLNPPVEDKET
                     LLALLRNRIPPGVDPAAEVKAAFLTEIAHGRKVSPLIDPQQAVFLLGTMLGGYNVRPL
                     VELLAHETLGSKAAEALSRTLLVFDAFDEVAALAHQGNENAKNVLQAWADATWFTERS
                     PLPERIDVVAFKVAGEINTDDFSPASHAFSRPDIPLHALSMGESRFPHGIEEIAKLRQ
                     QGKSIAFVGDVVGTGSSRKSACNSVLWHIGEDIPFVPNKRRGGLILGGQIAPIFFNTA
                     QDSGALPIRCDVTSIENGDALAVYPRQGKIENEAGKVLAAFTLSPETVADEVRAGGRV
                     PLIIGRSLTDRARRALGLDKTTIFLQPAKPKDPGVGFTLAQKIVGRACGLEGVRPATS
                     CEPTMTTVGSQDTTGPMTADELTELACLKFQADLVMQSFCHTAAYPKAVDAQMHRNLG
                     AFIQQRGGVALRPGDGVIHSWINRLLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAA
                     ALGKMPLDMPESVLVRFTGQLQPGITLRDLVNAIPYVAIQKGLLTVEKKGKKNVFNGR
                     ILEMEGLPDLSVEQAFELTDASAERSAAAATIRLNKEPVVAFLKSNAVLMRWMISQGY
                     GDAETLKRRADAVEAFIANPVLLEPDNDAHYAAVIEVDMTSITEPLLACPNDPDDVKP
                     LHEVAGARIDDVFLGSCMTNIGHYQAAARILDGKGFAASQLWVAPPTRMDVEQLKTEG
                     VYGIFAKAGARIEIPGCSLCMGNQARVRDGVTVFSTSTRNFDNRMGRGAKVYLGSSEL
                     GAVAAWKGKLPTVDEYFEAVNCLRESSEEVYRYLSFDQIEEYLAPRSVVQIRQ"
     gene            complement(3536..4069)
                     /locus_tag="PK710_17125"
     CDS             complement(3536..4069)
                     /locus_tag="PK710_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11503.1"
                     /translation="MKELQWGSLFVMLGCVVGCSKSPDSVCEHYMGLRKEAKLTTDAT
                     AADACKSNLSALKERSLHAWKCSSQCILGATDFKTADKCPVSCETQYGNNGESFEGAE
                     DTAVRERWDNGICGDTGKAGELKRLEDHLSERAAEKARVLEACVAECKPKHEVGTKEY
                     TSCLNRCQIKKLMDAAK"
     gene            4982..>6018
                     /locus_tag="PK710_17130"
     CDS             4982..>6018
                     /locus_tag="PK710_17130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11504.1"
                     /translation="MTVSSLSRVLMLCAFVSVSCSSSENGESSANGNHSDGGADSSDG
                     TGGTGGAGGSAGTGGTGGGILNTDSGNDQGTEEACVQQSVEATIEHRPIDIIFVIDNS
                     GSMSAEIEEVEAQINANFASIIDSAVPAIDYRVVMLSRYGAFSSRKICVAEPLGGAKD
                     DNQDGHCDAPLPNEPINTSKFFHHSMSIGSYNAFCQLLAGFSAADEFKLQPNGYGEIL
                     RPEAFKFITVITDDRVNCPPFDDLNTENGGKAAAQAFDKALRELSPEHFGKSENERNY
                     SFWSIVAMAPYEPTAAKPNGSPHPPDESLSPVTTEKCKPSAVNPGTGYQALSIMTGGY
                     RFPTCGLDYTE"
BASE COUNT         1321 a         1804 c         1645 g         1248 t
ORIGIN      
        1 ccttgctgcc cggcgtcagt cggtcgggtt ccaccatcgc cggaggcctt ctcggaggtt
       61 tgcgcccctc gttggccgta tcctattcct tcctcgtcgg tctgcccctc atcgctgccg
      121 ccgctgcaaa agatacgctc gacggaggat tcggacgctt gctgacggaa gtcggcgtgc
      181 taccgatcag tgtcgctttc gtggccgcat tggtcagcgg actgggctcg atcgccgctc
      241 tcaaactcgt ggtggggaaa cgacgtcttg tgtggtttgg tgggtactgc gcgctggtgg
      301 cgggcgtctg tttctggatg ggtttttcac aataaattca atatgttatc gtagtctcgc
      361 tcaatgccta ctggcgaatc tgcacgacgg atcggggcgc gagatattcc tcgatctggt
      421 cgaagctcag gtagcgatat acttcctcac tgctttcgcg caggcaattc acagcctcga
      481 agtattcatc caccgtgggg agcttgcctt tccacgccgc cacggccccc aattccgacg
      541 agccgagata caccttggcg ccgcgaccca tgcggttgtc gaagttgcgc gttgacgtgc
      601 tgaataccgt gaccccatcg cgaacgcgcg cttgatttcc catgcacaaa ctgcagcccg
      661 ggatctcgat gcgagcgccg gccttggcaa agatgccata tacgccttcg gtcttgagct
      721 gctcgacatc catgcgcgtg ggaggagcca cccagagctg ggaagcggcg aatcctttgc
      781 catcgagaat gcgcgccgcc gcttgataat ggccaatatt cgtcatgcac gaacccaaaa
      841 aaacgtcgtc gatacgcgct cccgcaacct catgcagcgg cttgacatcg tccggatcgt
      901 tgggacaggc caacaatggc tcggtgatcg acgtcatatc cacttcgatc accgccgcgt
      961 agtgcgcatc gttgtctggc tcgagcaaca cgggattggc gatgaatgct tccaccgcat
     1021 cggctcgccg cttcaacgtt tccgcatccc catagccttg cgaaatcatc catcgcatga
     1081 gcaccgcgtt gctcttcaaa aaggccacca ccggctcctt gttcaaccga atggtggccg
     1141 ccgcggcact gcgctcggca gaagcatccg tcaactcgaa agcctgctcc accgaaaggt
     1201 caggaaggcc ttccatctcc aggattcgcc cgttgaaaac gtttttcttc ccctttttct
     1261 cgaccgtcaa caggcccttt tgaatcgcca cataagggat ggcgttgacg agatcccgaa
     1321 gggtgatgcc tggctgaagt tgtccggtga aacgaacgag aacggactca ggcatgtcca
     1381 gaggcatctt gccaagcgcc gcggcaaaag ccaccaatcc agaaccggcg ggaaacgaga
     1441 ttccgatcgg aaaacgcgtg tgcgaatcgc cgcccgttcc caccgtatcc ggcaaaagca
     1501 aacgattgat ccacgaatga atgacaccat cgccgggccg taaagccacc ccgccgcgct
     1561 gctgaatgaa cgccccaagg ttccgatgca tttgcgcatc gaccgctttg ggataggccg
     1621 ccgtatgaca aaacgattgc atgaccagat ccgcctgaaa cttgaggcaa gccagctcgg
     1681 tgagttcatc cgcggtcatc gggcccgtgg tgtcctgaga accgaccgtg gtcatcgtcg
     1741 gctcgcaaga cgttgccgga cgcactccct cgagaccaca ggcgcgaccc acgatttttt
     1801 gggcaagcgt aaacccgacg cctggatcct tgggcttcgc gggctgcaaa aagatagttg
     1861 ttttatccaa tcccaacgct cgacgcgccc tatccgtgag ggaacgaccg atgatcagcg
     1921 gaacacgtcc gcccgctcga acctcgtccg ctaccgtttc cggactcagg gtaaaggcgg
     1981 ccagaacctt ccctgcttcg ttttcgatct ttccttggcg aggatagacc gcaagagcat
     2041 cgccattttc gatagaggtg acgtcacagc gaatgggcaa cgctcccgaa tcttgcgccg
     2101 tattgaagaa aatcggcgca atctgaccgc ccaggatgag ccctccgcga cgcttgttcg
     2161 gcacgaaagg aatatcctcg ccgatatgcc agagcacgct gttgcaggca ctttttcgtg
     2221 acgatcccgt tcccaccaca tcgcccacga aagcgatgga tttaccttgt tggcgcagct
     2281 tggcaatctc ttcgattccg tgcggaaaac gggattcccc catggaaagg gcgtgcaaag
     2341 ggatgtccgg acggctgaag gcatgactgg caggactgaa atcatcggta ttgatctctc
     2401 ccgcaacctt gaaagcaacc acatcgattc gctccggcaa aggcgatcgc tcggtgaacc
     2461 acgtcgcatc cgcccatgcc tgaagcacat ttttcgcatt ttcgttgcct tgatgagcca
     2521 gcgcggccac ctcgtcaaag gcatcgaata ccaatagcgt gcgggaaaga gcctcggcgg
     2581 ccttagaacc aagggtttcg tgcgccaaca gctcgaccag gggtcggacg ttgtatcccc
     2641 cgagcatggt cccgagcaga aaaaccgctt gctgagggtc gataaggggc gagaccttcc
     2701 gcccatgagc gatttcggta agaaatgccg cttttacctc tgccgcagga tctacccccg
     2761 ggggaattcg attgcggagc agagcaagga gagtctcctt gtcttccacg ggcggattca
     2821 gcaacaggtc gcatacgacg cgagcttgat ccgcgcgaag gggaagaggg ggaatgccca
     2881 gacgagcacg ttcttgcaca tggattcggt attcgctcag catgatgggc cgagcatccc
     2941 tccatgggca caagcattca accgtacaag aacccccaaa gcggcaaagg gcagagaacc
     3001 gtcaggacga aaaggcgccg agggtctgac agatcccttc atttttccct gaatggcaaa
     3061 gggatacgag cagggaatcc cctggacttt gacttgatcc ttgagcccat ttcgatcaag
     3121 ccttataaaa atatcaataa atacagttag ttgaacgctg cgtagtgatg ctcgtgggct
     3181 accaagcctc ctccagaagc tcagcgaagc attcatccta tgcgaggagc gtgatggtta
     3241 gcccagccag gatatgcctg acagatcccc tcattttccc gaattggaaa gggatataat
     3301 cgagaatcca ccgagccttg acgttgactt ggtccttggc cctccttgga gcaccaccaa
     3361 cagttgcccg gagctaccaa actttggact ggcgcactgc ggtgatcaaa atgggattac
     3421 cgtgagcgtt tctcgtgggc cgtgaccgtg aaggaaaggc ccgggaaaaa tgagggttat
     3481 gcgtcggccg tgagcgtgac cgttttatgt atgagtcctg ctcgtcgagc gcgccctact
     3541 tggcggcatc catgagcttc ttgatctggc atctgttgag gcacgacgtg tactccttcg
     3601 taccgacctc gtgcttcggt ttgcattccg cgacgcaagc ctcgagcacc cgcgctttct
     3661 ccgcggcacg ctcgctgagg tggtcctcga gcctcttgag ttcgcccgcc ttaccagtat
     3721 cgccgcagat gccgttgtcc cagcgctccc gtacagcggt atcctcagca ccctcgaagg
     3781 actcgccgtt gttgccgtac tgggtctcgc agctcacggg gcatttgtcc gccgttttga
     3841 agtcggtggc acccaagata cactgcgacg agcacttcca ggcatggagc gatcgctcct
     3901 tgagtgccga caagttgctc ttgcaggcgt cggccgccgt cgcatcggtg gtgagcttcg
     3961 cctccttgcg gaggcccata taatgctcgc acacggaatc gggcgacttg gagcagccaa
     4021 ccacgcagcc gagcatcacg aacagcgaac cccactgcaa ttctttcatg tctttcccct
     4081 tcaaagcgcg tgccacgatc acaaaaatgg tcccatacgc agatccacat gggtcacgac
     4141 agcccgaacg ctgccgcccg tcgcgcccat ctacccaaac ggcgcgcaac gcagtcggaa
     4201 atcggtgctg ccacagtcgt ctcggacacg tcaagacttt tccataggat agaaaggatg
     4261 aacgatttcg aattcgttca tgaaaaggag tcaaccaggt agaactgggc ggacccgagc
     4321 agggcagtca attcgagtct gctgtaggga atgcccagag ccgaacctcg tcgctcatca
     4381 gactcaggcc atcgcgacct ggacgtatac cgctggctgt cccgcgatag cgcgaatacc
     4441 cacgctaacg aacgccccca tggcagcagc acagcgttcg gagcgagcgc tcagcggaga
     4501 taggaatata tcaataaatt ccacgtgtta tacacaaatt tcgtgtggtt cgatgcaaac
     4561 atgccggatg aaccctcttc tctcttgaca ctttggcggg cacatcggca ccgacagggt
     4621 gcgtgctatc cgctctgacg ggctggagaa aaccgtggcc gccattccgg tcaagcgcct
     4681 gggtacgccc gaggaaattg gctctatcgt agcctgactg gccgacgacg acgccagaaa
     4741 cgaggcctga gacaagacga tgagcggcga cgctccgtgc ggtaagcgtc tcggcaagct
     4801 cggacgcgca gaggcaaagc gatggcgcac gtcgatcccc tcgaggctcc atggcaccca
     4861 tacgccgatt aatactgtaa ttttcaatat ttacagcaac agccgacttt tcgacagccc
     4921 catcatgctt ttctttcgat ccaatgctac tctggaaggc agcgcatgga aggagttggg
     4981 tatgacggtt tccagtctct cacgcgtatt gatgttgtgc gcgttcgttt ctgtttcatg
     5041 cagcagcagt gaaaacggtg agtcttctgc caatggcaac cattccgatg ggggcgctga
     5101 tagttcggac ggaacgggtg ggactggagg agctggagga tcagcgggca ccggcggcac
     5161 gggcggcggg atcctaaata ccgacagcgg caacgaccaa ggtaccgagg aagcgtgcgt
     5221 ccagcagtcc gtcgaagcta cgatcgaaca ccgccccatc gacatcatct tcgtcattga
     5281 caattccggc agcatgtccg ccgaaatcga ggaggtggag gcacaaatca acgccaattt
     5341 tgcctccatc atcgacagtg cggttccggc catcgactat cgtgtggtga tgctgagccg
     5401 atatggtgcc ttcagcagtc gcaaaatatg tgtggccgaa cccctgggtg gtgccaaaga
     5461 cgataaccaa gacggtcact gtgacgcccc ccttcccaac gagcccatca atacgtccaa
     5521 gttctttcat cacagtatgt ccatcggcag ttacaacgct ttttgccaat tgcttgcggg
     5581 tttttccgcg gcagacgaat tcaagcttca gcccaacggc tacggagaaa tcctgcgtcc
     5641 tgaggctttc aagttcatca cggtcatcac cgacgaccgt gtcaattgtc cgccctttga
     5701 cgatctgaac acggaaaatg gcggaaaggc tgccgcgcaa gcctttgaca aagccctgcg
     5761 cgagctttct cccgaacact ttggaaagag cgaaaacgaa cgcaattact cgttctggag
     5821 catcgtagcc atggctcctt acgagcccac cgcggcgaaa cccaacggtt ccccccatcc
     5881 cccggatgag tctctttctc ccgtaaccac agaaaagtgc aagccttccg ccgtcaaccc
     5941 tggaaccggc taccaggcgt tgagcattat gacgggtggc tatcgcttcc cgacctgtgg
     6001 gctggattac acggaaat
//
LOCUS       DAOSOR010000360         6007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13344_length_6007_cov_4.178931, whole genome shotgun sequence.
ACCESSION   DAOSOR010000360 DAOSOR010000000
VERSION     DAOSOR010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13344_length_6007_cov_4.178931"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PK710_17135"
     CDS             <1..442
                     /locus_tag="PK710_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11505.1"
                     /translation="PKALAPKIKGLKPAKPPRPLDARKILFRHQAISWLSWVSAWVAS
                     DDDAAEIPRLLSGTSILPRTENAWSSNPPSVRPEASPTAAWLEQSSMPAHKETEDSAS
                     PPENAIPLDVGKTQIGPGSPSAAQDSGSDVSKTDLEYPDKDSHA"
     gene            complement(568..1488)
                     /gene="prs"
                     /locus_tag="PK710_17140"
     CDS             complement(568..1488)
                     /gene="prs"
                     /locus_tag="PK710_17140"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOT11506.1"
                     /translation="MVPAPLVFSTNSYGYLRDEICKLGRLERGQVERRTFPDGEHYQR
                     ICESVSGRDVVLVGGTIDDGATLELFDLASTLVKYGADRLTLVIPYFGYSTMERAVRA
                     GEVVTAKTRARLFSAIPPASRGNRVFLLDLHTEGITHYFEGAITPRHISGRKVIAAAA
                     REIGGEDFVLACTDAGRAKWVESLANDLGVHAAFVFKRRLDETRTEVTAVSAQVRGKN
                     VVIYDDMIRTGGSLIHAARAYADAGAARLDAITTHGLFPADALEKLRSCGLLQRVVST
                     NSHPNACRLQDDFLQVISVADILVDELRAP"
     gene            complement(1609..3075)
                     /locus_tag="PK710_17145"
     CDS             complement(1609..3075)
                     /locus_tag="PK710_17145"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT11507.1"
                     /translation="MNTKADVTLLNLNMLCVRYVDRSESEVHLPLGPLYLAHALEKAG
                     YAVDFRDYQLVDRADKFSIEALRRFLSGAAPIVFVSCMANLLPYTLLALERYKLDHPD
                     AFLALGGVGAAGVERQVLDLCPWIDAISVGEGEVSGPALVRAAQRGRGTDGSWDLCDV
                     PGVLWRQKGAIFVQPAAARLRDLEAIAPAWHLVDLSRYAGINIITSRGCPFPCSFCSV
                     APVWGRKPVLRRVEGIVAEMEWLHERAGCELFLFQDEYFLSSSDRVRALCEAMQSRGL
                     RFRWKAFGRIDQTDEPTMRAMARGGCIELRFGVESGSDRILSRVVKGFDAQRAHEVLS
                     EAVGIFPGVDAFYMWGFPFETMQDFQQTLLHMIVSRTMGVRILPSLLSLLPQTDLYQS
                     LDPSEKLEFFPELFPEYMLTGHEVCDAGRVRIQPEHASIYRFIERNPHVFPGFFHIDV
                     EGNVLPKARLLQKFGFYASQDRQELLGAEVECCGAHSP"
     gene            complement(3072..4100)
                     /locus_tag="PK710_17150"
     CDS             complement(3072..4100)
                     /locus_tag="PK710_17150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11508.1"
                     /translation="MGIKAGSPNGITIVGGRPMHRAVQATELPQGVEQVLTIAALDPV
                     FRAQVARDPISAAAAKGIRLDPVEAALLQSMPAQRLAAMTERLIIPRSSGRRTFMKAV
                     SASVVAMVTGQAFLLCSGCTGADTFERDTEPEQRWMELAGYTCYVCIPTGIPEADAGT
                     VPVVLALHGEGETCLSSAQRWQAIGERAGFCLVAINWTEEARLPEQRKALVSHLQQIV
                     EQLGEQLRISTGDVYLTARGMAADMLVDAVYLGGSSLSCKAVNLLGGAPKGDWIGQAD
                     TLLAPLGATPPKLCYVLGKVDDDYAQGKAFAEALVARGVTVHRQELEEATDSAKLSFA
                     DIWSWMSA"
     gene            complement(4224..>4650)
                     /locus_tag="PK710_17155"
     CDS             complement(4224..>4650)
                     /locus_tag="PK710_17155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11509.1"
                     /translation="AAGSQSAAPIPVSTLPTSDRDKLHSLAQKVLGFIKNKDTEALVL
                     MAAPQDRQKMSQSLQAGQSMYETLFASGGWCGKAVEAWQEEVGETRIKDRQARVKFMD
                     LEDKRVAVVVFQKEDGVWYFQGVDNPSLDTYGSWGVSAR"
     assembly_gap    4651..4660
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5050..>6007)
                     /locus_tag="PK710_17160"
     CDS             complement(5050..>6007)
                     /locus_tag="PK710_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT11510.1"
                     /translation="KGIDVACPLGTLIAVTGVSGAGKSSLITGILFPALARQLHQAKV
                     TVGEHDRIDGIDAIDKCIHIDQSPIGRTPRSNPATYTKAFDLIRDIFSRTPEARALGY
                     GPGRFSFNVRGGRCEACEGDGMKRVEMHFLADVYVPCETCGGKRYHEATLRVQYRGMS
                     IADVLALSVDRAKQEFSAHPKLTRILQTLWDVGLDYVKLGQPAPTLSGGEAQRVKLSR
                     ELARTDTGKTLYVLDEPTTGLHFEDIHKLLGVIHRLVQAGNTVIVIEHNLDVIKCADH
                     VIDLGPEGGDQGGFIVAQGTPEQVASHRDSATGHALCAVLPP"
BASE COUNT         1423 a         1995 c         1431 g         1148 t
ORIGIN      
        1 ccccaaagcc ctggctccga aaataaaagg cctcaaaccc gccaaacctc cgcgccccct
       61 cgacgcacga aaaatattgt tccgacacca ggccatttca tggctctcct gggtcagcgc
      121 ctgggtggcc tccgatgacg atgccgcgga aattccacgt ctgctttccg gcacttccat
      181 cctgccacgg accgaaaatg catggtcatc gaacccccct agtgtacggc ccgaggcatc
      241 gccaacggca gcctggctcg aacaatcctc gatgcccgca cacaaagaaa cagaggacag
      301 tgcatcgcct ccggaaaacg caattcccct cgatgtgggc aaaacgcaaa tcggtcctgg
      361 ctctccgtct gccgcgcaag actcgggttc cgacgtatcg aagacagact tggaatatcc
      421 ggataaggat agtcacgcat gacgggctcg gaggatgggc cgacgcatga cgtatgcacg
      481 gaagccatca cgtcatccgt ggcagaggcg agcgtttttc catttcatcc aaggctcgct
      541 gtcgaaaaat aacggaagat caagtagtta cggcgcgcgc agctcgtcga ccaaaatatc
      601 cgccaccgaa atgacctgca gaaaatcgtc ctgcaatcgg catgcgttgg gatggctatt
      661 cgtgctcacc acacgctgta ataacccgca gcttcgcagt ttttccaagg catcggccgg
      721 aaacagcccg tgggtggtga tggcatcgag gcgcgcggcc cccgcatccg catacgcccg
      781 tgccgcatgg atgagcgagc cccccgtgcg aatcatatca tcataaatca ccacgttctt
      841 gccgcgcact tgcgcgctta ccgccgtaac ttccgtacgg gtttcgtcca gacgccgttt
      901 gaaaacgaag gccgcatgca cccccaaatc gttggccagg gactcgaccc acttggctcg
      961 acccgcatcc gtgcaggcca gcacaaagtc ctcaccaccg atctcgcgag ccgccgccgc
     1021 gatcactttc cttcccgaga tatgccgagg cgtaatcgct ccctcgaagt aatgcgtgat
     1081 gccttcggtg tgcaaatcaa gcaaaaatac gcggtttcct cgcgatgcgg gcgggatcgc
     1141 cgagaacaat cgcgcccgcg ttttcgccgt caccacctcc cccgcccgaa ccgcccgctc
     1201 catcgtcgaa tagccaaaat acggaatcac caacgtcaat cgatccgccc catacttcac
     1261 caatgtcgag gcaagatcga atagctccag cgtagcccca tcatcgatgg ttcccccgac
     1321 cagcaccaca tcacgccccg atacactctc gcatatccgt tgataatgct cgccgtcggg
     1381 aaaggtacga cgctctacct ggccgcgctc gagccgtcca agcttgcata tctcgtcgcg
     1441 aagatagccg taagagttcg tggagaaaac caaaggggcc ggaaccatga cagcttcata
     1501 ccctacccga agccgggagc gccaccatcg caacgcgctg tcggctcgtt cgatacgctt
     1561 tggtacgcca agcagatgta tccacgcgtc tgggcgcgtc gatatgcctt agggagaatg
     1621 cgcgccacaa cactcgactt ccgcaccaag cagttcttga cgatcctgcg atgcgtagaa
     1681 accgaacttc tgcaacaggc gagctttcgg aagcacgttg ccctcgacat cgatatgaaa
     1741 aaaaccagga aacacatggg gattccgttc gatgaaccgg tagatcgacg cgtgctccgg
     1801 ctgtatgcgg acccgcccag cgtcgcatac ctcatgtccc gtcagcatat actccgggaa
     1861 taactccgga aaaaactcga gtttttccga tggatccaag ctttgataca aatcggtttg
     1921 gggaagaagc gacaacaacg acggcaagat gcgtaccccc atcgtgcgac tcacgatcat
     1981 gtgcagcaac gtctgctgga agtcctgcat cgtttcgaaa ggaaatcccc acatataaaa
     2041 cgcatccaca ccgggaaaaa ttcctaccgc ttcgctgagt acctcgtgcg cgcgttgggc
     2101 atcgaaccct ttgacgaccc tcgagaggat tctgtccgaa cccgattcga ccccaaatcg
     2161 tagttcgata catccccccc gcgccatcgc tcgcatcgtg ggctcatcgg tttgatcgat
     2221 acgcccaaac gccttccatc gaaaccgaag ccctcgcgat tgcatcgcct cacacaacgc
     2281 gcgtacccgg tcggaagaag acaaaaaata ctcgtcttga aagagaaata attcgcagcc
     2341 cgcccgttcg tgcaaccact ccatctcggc cacgatgcct tcaacccgtc gcaggaccgg
     2401 ctttcttccc catacgggcg cgacagaaca aaatgagcaa ggaaaaggac atccccgcga
     2461 cgtaatgatg ttgatccccg cgtatcggga caaatccaca agatgccaag cgggcgcgat
     2521 cgcttcgaga tcccgaagcc gagccgcggc cggctgcacg aaaatcgcgc ctttttgcct
     2581 ccacaggacg ccaggcacat cgcataaatc ccagcttccg tctgtccccc gccctcgctg
     2641 ggccgccctt accaacgctg gtcccgaaac ctctccttcc cccaccgata tcgcatcgat
     2701 ccagggacat aaatcaagca cctgacgctc cacccccgcc gctcccactc cccccagcgc
     2761 aaggaaagca tccggatgat cgagcttgta tcgttcgagc gccaacaacg tatacggaag
     2821 cagattggcc atacagctta cgaaaacgat aggtgccgcc cccgataaaa accttcgcaa
     2881 cgcttcgatc gaaaacttat cggcccggtc cacgagctga taatcacgaa aatccaccgc
     2941 atatcccgcc ttttcgagcg catgcgccag gtaaagcggt ccaagaggca aatgaacctc
     3001 gctttcgctg cgatccacat accggacaca cagcatgttc aggttcaata aggtgacgtc
     3061 ggctttcgta ttcacgcgga catccaagac caaatatcgg caaaagaaag cttcgccgaa
     3121 tccgtagcct cctcgagttc ttgccgatgc accgtcacgc cacgcgccac gagcgcttcc
     3181 gcaaatgctt tgccctgcgc atagtcgtca tcgaccttgc ccaagacgta acataacttc
     3241 ggaggtgttg ccccaagagg cgcaagaagc gtgtctgctt gcccgatcca atcgcccttt
     3301 ggcgctcccc caagcagatt gaccgccttg caggatagcg aacttccccc caagtacacc
     3361 gcatcaacga gcatatccgc ggccatcccc cgcgccgtca agtacacgtc gcccgtggat
     3421 atcctcaatt gctcccccaa ctgctcgacg atctgctgta agtggctcac aagcgctttg
     3481 cgctgctccg gaagacgcgc ctcttccgtc cagttgatgg ccacaagaca gaatcccgcg
     3541 cgctcaccga tggcttgcca acgttgggcg ctggaaagac acgtctcccc ctctccgtgt
     3601 agcgccaata ccacgggaac agtccccgcg tccgcttcag gtatcccagt agggatacaa
     3661 acgtaacacg tatatccagc cagctccatc caccgttgct ccggctccgt atcccgctcg
     3721 aacgtatccg ctcccgtaca tcccgagcaa agaaggaagg cctggcctgt caccatcgcc
     3781 acaaccgaag cggataccgc tttcatgaac gttcgacgac cagaacttcg tggaatgatc
     3841 agccgctcgg tcatcgccgc caggcgctgt gcaggcatcg actgcaacaa agccgcttcc
     3901 accggatcga gacgaattcc tttggctgcc gccgccgaaa tgggatcccg tgccacttgg
     3961 gcgcgaaaca caggatcgag agccgcaatc gtcaacactt gctccacccc ttgcggcaac
     4021 tccgtggcct gcaccgcccg atgcatcgga cgacctccca caatggtgat tccattgggg
     4081 gaacccgctt tgattcccat gatatagcct ccgttggatg ggcaaaccgt tggcgtaaac
     4141 aaccgacggg agcgcaaaac catagcacca acccgctcac gcgcactcgg ccaaaaatga
     4201 ttcgcgctag caaaaaagcg tgttcaacgc gcggatacgc cccaggaccc ataggtatcc
     4261 agcgagggat tatccacacc ctggaaatac caaaccccat cctccttttg aaataccacc
     4321 accgccacgc gcttgtcttc aagatccatg aacttcacac gcgcttgtct gtctttgatc
     4381 cgtgtctcgc caacctcttc ctgccacgct tccaccgctt tgccacacca cccaccggaa
     4441 gcgaatagtg tctcgtacat cgattgcccc gcctgaagcg attggctcat cttctgccga
     4501 tcctgcggcg cggccatcag aaccaacgcc tccgtatcct tgttttttat gaatccaagg
     4561 actttttgcg ccagcgaatg aagcttgtcc cgatcgctcg tgggtaacgt cgataccgga
     4621 atcggagccg cagattgcga gcccgccgct nnnnnnnnnn aacgtcaacc cgtcgtcccc
     4681 caacgatcct gaatctcgct tggtgtcttt gcacccaatc gatagcccga caacaacaca
     4741 catcgcagga tagacaattc gaggatacat catccgacaa cgctacgttc gcttcgacaa
     4801 ctccgtcaac caccgctcgt tcgtaacccc gttgcgtacg cgcgacatgg cacgttcgca
     4861 acgacccgca tcacgatcga tacagatggt tcgatgacga taccatcacc ccacggatag
     4921 ggatgcttcg cggctgggat cgaaatcgaa gcaagaaaaa gacatcggtc taagcccttt
     4981 ccggatacat cgataagctt ggacatgcga cttcgcacac gcggaagaac ctgcgtacgg
     5041 cggatgaaat catgggggaa gcacggcgca aagcgcatgc cccgtggccg aatcccgatg
     5101 acttgccacc tgctcaggag tcccttgcgc aacgataaaa ccgccttgat cccctccctc
     5161 gggacctaaa tcgatgacat gatccgcaca cttgatgacg tcgagattat gctcgatgac
     5221 gatgaccgtg ttgcccgctt gcaccagacg atgaatgacc cccaacaatt tatgaatatc
     5281 ctcgaagtga agccccgtcg tgggttcatc gagaacgtat aacgttttgc ccgtatccgt
     5341 cctcgcaagc tcgcggctga gcttcacgcg ctgggcttcc cctcccgata gcgtaggcgc
     5401 aggttgcccg agctttacat agtccaaacc cacatcccac aaggtttgaa ggattcgtgt
     5461 gagtttcgga tgggcagaaa actcctgttt cgctcgatcc accgaaagag ccaacacatc
     5521 cgcgatcgac atcccccggt attgcacccg aagggttgcc tcgtgatacc gttttccacc
     5581 gcaagtttcg caaggcacat acacatcggc aagaaagtgc atctccacgc gcttcatccc
     5641 atcgccttca catgcctcgc atcgacctcc acgcacgttg aaagaaaacc tccccggacc
     5701 atagcccaaa gcccgcgctt caggcgttcg cgaaaaaata tcgcgaatca gatcgaaggc
     5761 cttcgtatag gtcgcgggat tcgaacgagg cgtacgccca atcggactct gatcgatatg
     5821 aatgcacttg tcgatcgcgt cgatcccatc gatgcgatca tgctctccca ccgtgacttt
     5881 ggcctgatgc agttgtctcg ccagcgccgg aaatagaatt cccgtgatca aggaactctt
     5941 tcccgccccc gacacgcccg tgacagcaat gagcgtgccc aatgggcacg ccacatcgat
     6001 gcccttc
//
LOCUS       DAOSOR010000361         5960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13471_length_5960_cov_4.527519, whole genome shotgun sequence.
ACCESSION   DAOSOR010000361 DAOSOR010000000
VERSION     DAOSOR010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13471_length_5960_cov_4.527519"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="PK710_17165"
     CDS             <1..1292
                     /locus_tag="PK710_17165"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOT11511.1"
                     /translation="ARTRKTVLGDNPFSSEPAAPETKNERAPTSPTADGSALPHPSPP
                     EKPARKAKTKGATAAAQIQPIPSRTRKPTATKTPSNTQPKKSQTTPASQPSVSKKLVP
                     SSPQPVVEKQTSAEEPSERKPSFSIAEEPRDRSEQVTEPIERSDAGPEAAVSDYFSRQ
                     WGRQGMRNRAETIDDFGYDRDAETHLSPLLDFLHDRYFRVEVEGAHHIPASGRALIVA
                     NHAGAFPYDGIMLRTALRKSLPADRQLRWLVEDHWFYMPFVGAFLNRMGAVRACQDNA
                     QRLLQQERLVAVFPEGAKGTGRLFKDKHKLQRFGRGGFVRLCLRTQTPIIPCAILGGE
                     ESMPLLCRIESFAHLIGVPHIPITPTFPWLGVLGAIPAPTRWQFIFGEPIQIERHSPS
                     GADDTVLVGRLTERVRATIQVTLDRASASRRSVWFGG"
     gene            1289..2272
                     /gene="truB"
                     /locus_tag="PK710_17170"
     CDS             1289..2272
                     /gene="truB"
                     /locus_tag="PK710_17170"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOT11512.1"
                     /translation="MKPSSNSSSSSSSKPSGLLLIDKPSGPTSHDVVARVRRALQTRS
                     VGHAGTLDPMASGVLVVMVGSCTKLSSYLTLEDKVYRARILLGYATDTLDAEGSITDS
                     ATVPAALLSILAHIEKGHFEQNDRQALHHALVMERNRTLQVPPSYSAIKRDGVASYQR
                     ARRGEQLELPARDVRVHRLDLVGATMDPPSVIVEVHVSKGYYVRALARDLGRSLELPS
                     HLMELRRLRSGAFGLEDTITLDADARAMNDAMLSVEQTARRCLPVATLTRQGACRALR
                     GQALGDDDFAHPPIERVSAWFSEEGRLVAIGDRSRGRPTVCRAFLHIDEMC"
     gene            complement(2269..2886)
                     /locus_tag="PK710_17175"
     CDS             complement(2269..2886)
                     /locus_tag="PK710_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11513.1"
                     /translation="MSLAPIFMSHASIPRAKAPILDNAKASLLSPCFRSCLRSIACET
                     EAQPLSNPIAREPPTILRPSFKDKPYESDDSPPSEDSSSHDDVGDSFRCREPSAPVLE
                     PCLPIASSEGQEQRQQWARVHTLEQWFPSIVRKAAWSISADRRSAILRLEIGTGAMRG
                     AIVLIRADPHSVHVELDAPPGIDLEAWKNRLRRRLASHRLAMIVS"
     gene            2947..4293
                     /locus_tag="PK710_17180"
     CDS             2947..4293
                     /locus_tag="PK710_17180"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOT11514.1"
                     /translation="MSWDQLGQELALVMETQTAMAAHWGGAVFSALLGSVFSAATVTL
                     VAMPTTRLTVLRDDAQGAHRDALNRYLKNPIRIRSRWLVGRVACTAMTAVLIGQAILP
                     LNEGWALPIAVLGTLATYGLLTEVTTSLVRRHPSKAAPFLLRLLFPFEMLLAPVAWPL
                     EWIARLTGKLIPHREEDARYREHEVGLLLEEGQRDGTLEEERVQMLKNMLEFEDLMAV
                     EVMVPRTSVTAIDAETTLEEALRVISSKGHSRYPVYRESVDNIIGLLYAKDLFRVVED
                     QNLQGKKVGEIVRTPVNFVPEAKPVSALLREMRARRLHMAVVVDDYGGVAGIVTLEDI
                     IEEIIGDIRDEHDVEEDPIVELGNGGMLVDAAMAIDDLSEMLGTEFAEEGDFVSLGGW
                     IIHRAGRVPEPGAELEAWGMRFIVREADERKISKVEIHRKDGQSVPPRAQEPFVAT"
     gene            complement(4324..5478)
                     /locus_tag="PK710_17185"
     CDS             complement(4324..5478)
                     /locus_tag="PK710_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11515.1"
                     /translation="MSEKKVKIDLKTRLGRASKGATSTPGVVPPPAMGIAPPPGIVSG
                     IPAPPFAAQPKKPAGPKIDQDEPFAAVSAGAVAPQAQEIKVEIGHEVVQAQKKGFVKV
                     VLAAVLAGLLGAGIGAAIGSLQARGAQDAVAVHDAQSLIEPINKAKENIEALQTKVAD
                     GTKSIYKDKKFPENFAKELSTIDIGFSASDLGGKALHRLKPALMRDLFDFARDAQELE
                     TRKESLRRLFDARKAAIIELIEQGKNPQISYGVFVQKDRNGNPVGTFVPLAKPYRFDE
                     KSWPDELSLNTGSEVVKAQRYKTGEPFTQAPRREGDKPTVYTIPLEPDGVVKVFPPRL
                     SQRIEEDLKAMAALITGTTHGEVAADQEKRGLLKLAEEITRELSTIGAKR"
     gene            5706..>5960
                     /locus_tag="PK710_17190"
     CDS             5706..>5960
                     /locus_tag="PK710_17190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11516.1"
                     /translation="MPPVDYTTKHTASLDGTEVSYQVAGSQGPWIVVANGLGAGNSAW
                     RQTVEYFADQARFLLWDYRGLVSPEENESTATLPARVDTHA"
BASE COUNT         1220 a         1685 c         1790 g         1265 t
ORIGIN      
        1 tggcccgaac acgcaaaacg gttctcggtg ataacccttt ttcttccgaa ccggcagcgc
       61 ccgaaacaaa aaatgagcga gcgccgacct cgccaaccgc cgacggcagc gccttgcccc
      121 atccttcacc gcctgaaaaa cctgctcgaa aagcaaaaac aaaaggcgcg accgcggctg
      181 cccagatcca gccgatacct tcccggaccc gcaaacccac agcaacaaaa acaccgtcaa
      241 acacgcaacc caaaaagtca caaacaaccc ccgcttccca accctccgtt tccaaaaaac
      301 tcgtgccgtc ttcgccccag ccggtcgtcg aaaaacagac atccgccgaa gagccttcgg
      361 aacgaaaacc atcgttttcg atcgccgaag aaccacgcga tcgatccgaa caggtgaccg
      421 agccgatcga acgatcggat gccgggccgg aagccgcggt cagcgattat ttttcgcgcc
      481 aatggggacg ccagggaatg cgtaaccgcg cggaaactat agatgatttt ggttatgacc
      541 gcgatgcgga aacgcacctc tcccccctgc tcgacttcct gcatgaccgc tactttcgcg
      601 tcgaggtcga aggcgctcac catatccccg ccagcggtcg cgccttgatc gtcgccaatc
      661 acgccggagc atttccctac gatgggatca tgctgagaac cgcgttgcga aaatcgcttc
      721 ccgccgatcg acaacttcga tggctcgtcg aagaccattg gttttacatg cctttcgtgg
      781 gagctttttt gaatcgaatg ggagccgtgc gggcatgcca agacaacgcg caacggcttc
      841 ttcaacaaga acggctcgtc gccgtctttc ccgaaggcgc caagggaacc ggacgattgt
      901 tcaaagacaa acacaagctc cagcgatttg gtcgcggcgg cttcgttcgc ctttgcttgc
      961 gtacccaaac acccatcatc ccttgcgcca tcctcggcgg ggaagaatcc atgcccttgt
     1021 tgtgccgcat cgaatccttc gcgcatctca tcggtgtgcc gcatatcccc atcacgccga
     1081 cattcccatg gcttggagtg ttgggcgcca ttcccgcgcc gacccgatgg caattcattt
     1141 tcggcgaacc cattcagatc gaacgccata gcccttccgg cgcggacgat acggtgttgg
     1201 tcggccgctt gaccgaaagg gtccgggcca cgatacaagt aactctcgat cgcgcatcgg
     1261 cttcacgtcg ctccgtgtgg ttcggcggat gaagccatcc tcgaattcat cctcgagttc
     1321 atcttcgaaa ccgtccgggc tcttgctcat cgacaagcct tccggtccta cgagccacga
     1381 cgtggtggcg cgagtgcgtc gtgccttgca aacgcgatcg gtgggacacg cgggaacgct
     1441 cgatccgatg gcgtcgggag ttctggtggt gatggtcgga tcgtgtacca agctatctag
     1501 ctatctcacg ctcgaagaca aagtatatcg agcgcgaatt ctgctcggat acgccaccga
     1561 taccctcgat gcggaaggaa gcatcaccga ttccgcaacc gttccggcag ccctgctttc
     1621 catcctcgcc cacatcgaaa aaggacactt cgagcagaac gatcggcaag cgttgcacca
     1681 tgccctggtc atggaacgaa atcggacgtt gcaagtgccg ccttcgtact ccgcgatcaa
     1741 acgagatggg gtggcgtcgt atcaaagagc gcgacgaggt gagcaattgg agttgcccgc
     1801 tcgtgatgtg cgggtgcatc gtctcgacct tgtgggagcc acgatggatc caccctcggt
     1861 gatcgtcgaa gtccatgttt ccaaagggta ttatgtgcga gcgctggcgc gtgacctggg
     1921 ccgttcgctc gaacttccct ctcatctgat ggagcttcgc agactacgaa gtggagcgtt
     1981 tggtctggag gatacgataa cgttggacgc ggacgctcga gcgatgaacg atgcgatgct
     2041 atcggtggag cagacggcac gacgatgtct tcctgtcgca acgttgacga ggcaaggtgc
     2101 ctgtcgagcg ctgcgaggtc aggcgcttgg ggatgacgat ttcgctcatc cgccgatcga
     2161 gcgcgtatcc gcttggtttt ccgaggaagg aaggcttgtg gcgatcggag atcgttcccg
     2221 ggggcggcct acggtgtgtc gagccttctt gcatatcgac gagatgtgct aagacacaat
     2281 catggcgagt cggtgagatg cgaggcgacg acggagacga ttcttccagg cctccagatc
     2341 gataccgggt ggagcatcaa gctcgacgtg gacggagtgg gggtccgctc gaatgagcac
     2401 gatggctcct cgcatggccc cggtgccgat ttccaatcgc aggatggcgc tacgacgatc
     2461 ggcggaaatg gaccatgcgg cctttcgtac gatcgatgga aaccattgtt cgagggtgtg
     2521 gacgcgcgcc cattgctggc gctgttcttg tccctcggac gacgcgatcg gcaagcatgg
     2581 ttccaagacc ggtgcggacg gctcgcgaca gcgaaatgag tcacccacgt catcgtggct
     2641 cgaggagtcc tcggatggcg gcgaatcgtc ggactcgtat ggcttatcct tgaagctggg
     2701 gcgcaggatg gtgggaggct cgcgcgcgat ggggttcgaa aggggttgtg cttcggtttc
     2761 gcaagcgatc gaacgcaagc acgatcggaa gcaaggggaa agcagcgatg ctttggcgtt
     2821 gtcgaggatc ggtgctttcg cccgtgggat ggacgcatgg ctcatgaaaa taggcgctaa
     2881 cgacacgaaa ggagttcgga cagttatacg gatggttgcg aaatacttgc tacaggggag
     2941 cgttccgtga gttgggatca actcggccaa gaacttgcgc tggtcatgga aacgcagaca
     3001 gcgatggctg cccattgggg cggagcagtc ttttccgcgc ttctgggaag cgtattcagt
     3061 gcggccacgg tcacgctcgt cgccatgccc accactcgtc tgacggtgtt gcgcgatgac
     3121 gcccaagggg cccatcgtga cgcgttgaat cggtatttga aaaacccgat acggatccga
     3181 tcgcgatggt tggtggggcg tgtggcatgc acagcgatga ccgcggtgct cattggtcag
     3241 gccatccttc ctttgaacga gggctgggct ttgcccatcg ccgtgttggg tacgctcgcc
     3301 acctacgggc tattgaccga agtgacgaca tcgttggttc gccgtcatcc ttcgaaagcg
     3361 gctccgtttc tgcttcgcct gctatttcct ttcgaaatgc tgctcgcgcc agtggcatgg
     3421 ccgttggaat ggatcgctcg attgacgggg aaactcattc ctcatcgaga agaggatgct
     3481 cgttaccgag agcatgaggt ggggctgctg ctcgaggaag gtcaacggga tggcacgctt
     3541 gaggaagagc gtgtccagat gctaaaaaac atgttggaat tcgaggactt gatggctgtg
     3601 gaggtaatgg ttccgcgcac gagcgttacc gccatcgatg cggagacgac cttggaagag
     3661 gcgcttcgcg tcatttcgag caaaggtcat tctcgctatc cggtgtatcg cgaatcggtg
     3721 gataacatca ttgggttgct ctacgcgaag gacttgttcc gcgtggtgga agatcagaat
     3781 cttcaaggga aaaaagtggg ggaaatcgtc cgtactcccg tcaatttcgt tcccgaagcc
     3841 aaacccgtat ccgcgctgtt gcgggagatg cgcgcgcgac gtctgcacat ggcggttgtg
     3901 gtggatgatt atggcggtgt ggcgggcatc gtgacgttgg aggacatcat cgaagagatc
     3961 attggcgaca ttcgcgacga gcatgacgtg gaagaggatc cgatcgtgga actcggcaac
     4021 ggtgggatgc tggtggatgc ggcgatggcc atcgatgatc tatcggagat gttgggaacc
     4081 gaatttgcgg aggaggggga tttcgtatcg cttgggggat ggatcattca tcgggcggga
     4141 cgtgttccgg agccgggagc cgaactcgag gcgtggggta tgcgtttcat cgttcgcgag
     4201 gcggacgagc gaaagatttc gaaagtggag attcaccgca aggatgggca gagcgtgcct
     4261 ccgcgggcgc aagaaccgtt cgtggcgacg tgaaataatc tttggagggc agcgtgacgg
     4321 gcgctatcgt ttggcgccga tcgtggagag ttctcgggta atttcctcgg cgagcttgag
     4381 cagacctcgc ttctcctgat ccgcggcgac ctcaccgtgg gtcgttcccg tgatgagggc
     4441 agccatggcc ttgagatctt cttcgatccg ctggctgaga cgaggaggga ataccttgac
     4501 gacaccatcg ggttcgagag gaatggtgta aaccgtgggt ttatcccctt cgcgtcgagg
     4561 tgcctgggta aaaggctcgc cggtcttgta tcgctgggct ttgacgactt cactgccggt
     4621 attgagactc aattcatcag gccatgattt ttcgtcgaag cgatacggtt tggccaaagg
     4681 aacgaacgtg ccgaccggat tgccgtttcg gtccttctgc acgaaaacgc cataactgat
     4741 ctgcggattt ttcccttgct cgatgagttc gatgatggcg gctttgcggg catcgaatag
     4801 acgtcgcaag gattccttgc gcgtttcgag ttcttgcgcg tcccgcgcaa aatcgaataa
     4861 gtcgcgcata agagcaggct tgagacgatg caaggctttg ccgcccaggt cgctcgcgga
     4921 aaaaccgatg tcgatggtcg acaattcctt agcgaagttt tcggggaatt ttttgtcttt
     4981 gtagatgctt ttggtgccgt cagcgacttt ggtttgaagc gcttcgatat tctcttttgc
     5041 cttgttgatt ggctcgatga ggctttgtgc gtcatggacg gcgacggcat cttgcgcgcc
     5101 tcgggcctgg agagaaccga tcgcggcacc aataccggcg cccaacaggc cagcgagcac
     5161 ggcggcaagc accaccttca cgaagccctt tttctgggcc tgcaccacct catggccgat
     5221 ttcaactttg atttcttgcg cttgcggcgc tacggccccc gccgaaaccg ccgcgaacgg
     5281 ctcgtcctga tcgatcttcg gaccggcagg cttcttcggc tgggctgcaa aaggaggggc
     5341 gggaatacca gacacgatac ccggcggagg agcgatcccc atcgcggggg gagggaccac
     5401 acccggcgtc gacgtggccc ctttcgaagc cctgccaaga cgtgtcttta ggtcgatttt
     5461 gactttcttc tcggacatct gctctttgcc agcaagttgc gaaatgggaa ttgaggcttt
     5521 ggaaccgagc gcagaacgta gcgggcagac atgcattctg tcaacgtttt gtgcaggcgg
     5581 cacaacgaca gacgggacta tcgccgggac tgcccgctcg aatcgcttgc ctcctgccca
     5641 agccaatccg tacgcaccca tttttacctt gctctccacc ccttcccgtc gaataacccc
     5701 cgctcatgcc ccccgtcgac tacacgacca aacataccgc ctcgctggac ggaaccgaag
     5761 tctcttacca agtggccggt agccagggtc cctggatcgt cgtggccaat ggccttggcg
     5821 ccggcaattc cgcctggcgc caaaccgtcg aatacttcgc ggaccaggcc cgattcctgc
     5881 tctgggatta tcgaggcctc gtttcccctg aggaaaacga atccaccgcc actctccccg
     5941 cccgcgtcga tacccacgcc
//
LOCUS       DAOSOR010000362         5957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13480_length_5957_cov_4.485768, whole genome shotgun sequence.
ACCESSION   DAOSOR010000362 DAOSOR010000000
VERSION     DAOSOR010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5957
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13480_length_5957_cov_4.485768"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PK710_17195"
     CDS             <1..680
                     /locus_tag="PK710_17195"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOT11517.1"
                     /translation="RKLLKHNNNPLLRSLRVGHTCSESPTGQQVDLVMMIASAYDPSL
                     VQQARSMGKHVWIYNGELPHTGTFLTDGWYASLRANAWIQQKYDIERWFYWESTFWTD
                     GNRGGHGPYDPLLWSETFHNAQGDHCNGDGVLVYPGKQVVEGYRTLGFDGVLSSIRLK
                     QWRRGISDAGYLQLVQKIDRETSDRIMTKMIPGALQQVSGGIPLWPREGAAWTEARRE
                     LFDKLRK"
     gene            781..>1112
                     /locus_tag="PK710_17200"
     CDS             781..>1112
                     /locus_tag="PK710_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11518.1"
                     /translation="MSCRMGLLLLVMFTSPGCASGNDVASAPSPSPKDASIDTEGGMG
                     DGANPSDASTSDAWPGDSPDPDTSEPEGSAPDSSPDTPCVGSCDGKVCADDGCGQPCG
                     TCDQGKIC"
     assembly_gap    1113..1122
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1123..2286
                     /locus_tag="PK710_17205"
     CDS             <1123..2286
                     /locus_tag="PK710_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11519.1"
                     /translation="TCVCDPALPPGWPASFSPWSHNPVLVPTSSTANHGSDNVYAPDI
                     HDVGGVSVMFYGAQGGDGHDRIYLAWSKDGAEWRKYPSDASPSPVLDRGSSNHVNDPS
                     VVHAGSTWRMYYTDAPTAENDRIWLAEGSSPTKFSKHSEVLSPGPAGSWEANKVGRPS
                     VLLENGEYRMWYDGSGSSGRHIGLATSTDGVNFARFAGNPIFMHAGAIDVKNVGGVYV
                     MLREAGDGTYWATSVNGTCWVDRGKLFGLSGDSYDAFGQVTPFLELKAGKPRAVWFGG
                     ASVSSWNRNRVAVAWSSEVKPPSGGGCSACTLWGATCTHACAQAGAGAVGVCAQPGST
                     NPSACCACSNDGCEGCRGSAKDCHEACVGAGKPGGWCGFPGSTNPSQCCACLD"
     gene            complement(2391..2978)
                     /locus_tag="PK710_17210"
     CDS             complement(2391..2978)
                     /locus_tag="PK710_17210"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOT11520.1"
                     /translation="MSERRMEQQITGGRTLSVCRGDLTEEFVDAIVNAANERLAHGGG
                     LAGAIVKKGGMEIQTESWALAPVPTGRAAATGGGRLPCRRVIHAVGPIWKEYEPQQAD
                     RLLASAVQQSLSVASKEGLVSIAIPAISSGIFGFPKDRCAKVMIDAVFDFFDQHGDST
                     LRDIRLCNFDEPTVACFERALRERLAPVTAESSSS"
     gene            3098..5128
                     /locus_tag="PK710_17215"
     CDS             3098..5128
                     /locus_tag="PK710_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11521.1"
                     /translation="MVPLLVSESRLHHGSEIVLNGTTYRLNFLTGDDTRLHVPSRNGE
                     SVHGEALRFDQALRGLHELAQRITLLNERLLDPLDGLTNRCVQTILTVPEVSASFKER
                     LAPWAFALFKHGSNVHGERGCVLVPPASREQFEKQIQGLSARQRGQYRWRDFLDHIYD
                     ATPLVTQGFYLFDRPEDRLRFEEALGSEYALIRKKLHRIFNDALGNDGAIPARQWKRF
                     DELSQQALSMQQTFLEPALSAAEGEVRAIATRVRIRCYEGGELRFLVRPYSFEFAEQF
                     DEMAELLERVRMQVPPHAIELREVLADLSDWCRARTTDESWAECLPSWIAASDPTNLI
                     DVNLTVEEKVSRLGAKGGIQLVVSAFETVPEPLRPIVSMLNDSNQGANLNVVWLKHLL
                     VGGGASNMTMAGEKLPDSEGRDCYKVMTFTNAVRSSMVESQAELMVASTHFSEDDIPR
                     LAHAANLLVLLHEVGHTFGDFAEFLGEYGASVEETHAEASVIFLAHRWAPQSLVDLIG
                     LTACWTPVHRCLQGAAEAHSHSDIVLFDEILRAGGVACVERDGRPIVAPMSADEAVRA
                     AFDLSLRMRLWELGIPSGEHGTWWVRFDPNDPALDVRVIQRARKAVELLGQEQRRAWQ
                     TQVFEECKVFFAQSRLEELSRPLQPIIERLPRYQPLSIIPTDERFRLVASFV"
     gene            5465..5656
                     /locus_tag="PK710_17220"
     CDS             5465..5656
                     /locus_tag="PK710_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT11522.1"
                     /translation="MARKITEECINCGACEPECPNEAISAGDDIYIIDPDKCDECEGR
                     ETVGCVEVCPVADEAIVMA"
     gene            5748..>5957
                     /locus_tag="PK710_17225"
     CDS             5748..>5957
                     /locus_tag="PK710_17225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11523.1"
                     /translation="MSMIPTRFAKQAWSAFAVPMVFSLYSCVETSGSAWMRQPLTPDD
                     EPSSVMVEPRYRGSGTRKDTRTHTLG"
BASE COUNT         1237 a         1563 c         1782 g         1365 t
ORIGIN      
        1 ggcgtaagtt attgaaacat aacaataatc cacttcttcg ctcgcttcgg gtgggacata
       61 cttgctcgga atcgccgacg ggacagcaag tggatttggt gatgatgatt gcctctgctt
      121 acgacccatc gctcgtgcag caggcgcgtt cgatgggcaa gcatgtatgg atttacaacg
      181 gtgaacttcc gcacacggga acgttcctca ccgacggctg gtatgcctcc ttgcgcgcca
      241 acgcctggat tcaacagaaa tacgatatcg agcgatggtt ttattgggag tccacgtttt
      301 ggacggatgg caatcgaggt gggcatggtc cttatgatcc gctgttgtgg tcggagacat
      361 tccacaacgc gcaaggcgat cattgcaatg gcgatggcgt gctcgtgtat ccgggcaagc
      421 aggtggtgga aggttatcga acgctggggt tcgacggtgt tttgtcttcg attcgactga
      481 agcaatggcg ccgcggcatc agcgatgctg gttatttgca gttggtgcaa aaaatcgatc
      541 gtgaaacttc agaccgaatc atgacaaaga tgatacctgg agcgttgcag caagtgagcg
      601 gtgggatacc gctgtggccg agggagggag cggcctggac ggaagcgcgt cgtgagctat
      661 tcgacaagct tcgaaagtga acggcagcca cggtgccgaa caaaaccctg cggggagcac
      721 aaacggtcca ttatcctcgt cgagcgatcg tgataatcta tgttttccgg aggtcgcgcg
      781 atgtcctgcc gcatgggatt gttgttgctt gtcatgttca ccagcccggg ttgtgcgagc
      841 ggcaacgacg ttgcatccgc accgtcgcca tccccgaagg atgcttcgat cgacaccgaa
      901 ggtgggatgg gggatggggc caatccttcg gacgcatcca cgtctgatgc ctggccgggg
      961 gacagtccgg atccggatac ttcggaacct gaaggttcgg cgccggattc ctcgcccgac
     1021 acaccttgtg ttggttcctg tgatgggaag gtatgcgccg acgatggttg tggacagcct
     1081 tgcggtacct gcgatcaggg aaaaatatgt gannnnnnnn nnacttgtgt ttgtgatccc
     1141 gcactaccac ccggttggcc tgcttcgttc tctccatgga gccataatcc cgtgttggtt
     1201 cccacgtctt cgaccgccaa tcatggctcc gacaacgtct acgcgcccga catccacgat
     1261 gttggaggcg tttccgtgat gttttatggg gcgcaagggg gcgatggtca cgatcgtatt
     1321 tatctagcct ggtccaaaga cggggccgag tggcgaaaat atccttcgga tgcatcacct
     1381 tcgccggtgc tcgatcgtgg atcgtccaac catgtcaacg atccgagcgt ggttcacgct
     1441 ggttcaacgt ggcgcatgta ttatacggac gctcccacgg cggaaaacga tcggatctgg
     1501 ctggcggaag gctcttcgcc gacgaagttt agcaagcact cggaagtatt gtctccggga
     1561 ccggcgggaa gttgggaagc aaacaaggtc ggacgtccat cggtgctatt ggaaaacggc
     1621 gagtatcgta tgtggtacga tggctcgggt tcgagcgggc gtcatatagg acttgcaaca
     1681 tcgacggacg gcgtgaattt cgcgcgattt gcgggcaatc ccatttttat gcacgccggt
     1741 gccatcgatg tgaaaaatgt ggggggcgtc tatgtgatgc tccgtgaggc gggcgatggg
     1801 acctattggg ccacgagtgt gaatggaacc tgctgggtcg atcgcggtaa gctctttgga
     1861 ttgagcggag acagttacga tgcgttcggt caagtcactc catttttgga attgaaagca
     1921 ggaaaaccac gagcggtatg gtttggaggc gcgtccgtat catcgtggaa tcggaatcgc
     1981 gttgctgtcg cgtggtctag cgaagtcaaa ccaccaagcg gcggagggtg ttccgcctgc
     2041 acgctttggg gggcaacgtg tacgcatgct tgcgcccagg cgggagccgg tgctgtcggg
     2101 gtctgcgcac agccgggttc caccaatcca tcggcatgct gcgcttgctc gaacgacgga
     2161 tgcgagggtt gtcgaggcag tgccaaagac tgtcatgaag cctgcgttgg cgcggggaaa
     2221 cccggtggat ggtgtggttt tccgggaagc accaacccca gccaatgctg cgcttgcttg
     2281 gattgaatcg atcgcaaatc gctcgagcca acggcatcag cgtctcgcaa tacgagcctg
     2341 cctggaaaaa tctggtaccg gtgtggacgg aagccatcac cgaagcggta tcatgatgat
     2401 gagctttcgg ccgtcacagg agctaggcgt tcgcgtaacg ctcgttcgaa acacgccacg
     2461 gtgggctcat cgaagttgca caagcgaata tcgcgcaacg tactatcgcc gtgttgatcg
     2521 aaaaaatcga aaacggcgtc gatcatcact tttgcacacc gatccttcgg aaacccaaaa
     2581 atgcccgaac ttatcgccgg aatggcaatc gacacgaggc cttcttttga agccacggaa
     2641 agagattgtt ggactgccga ggcaagaagc cggtcagctt gttgaggttc atattctttc
     2701 catatcggac cgaccgcatg gatgacacgt cggcagggca agcgcccccc gcccgtggcc
     2761 gccgcccgtc ccgtgggcac aggagcaagc gcccagcttt cggtttgtat ctccattccc
     2821 ccttttttta cgatggcccc cgccaaccct ccaccatggg caagacgctc gttggccgca
     2881 ttgacgatcg catcgacgaa ctcttccgtc agatctcccc gacagacaga cagcgttcga
     2941 ccaccagtga tttgttgctc cattcgtcgt tccgacatga tgggatacat tatcacaggc
     3001 gacgcgagca tgaacacgcg ttcccttcgc ctccgagctt gacgccagcg caaagggcat
     3061 cccagatcat cgtctgccca actcgatgag gttacctttg gttccgctcc ttgtctccga
     3121 gtcgcgtctt caccacggtt ccgagatcgt cctcaacggt acgacgtatc ggttgaattt
     3181 tctgacgggt gatgacacgc gtttgcatgt tccatcgcga aacggcgagt ctgtgcatgg
     3241 tgaggccctt cgtttcgatc aggctctacg cggtttacac gagttggcac agcggatcac
     3301 gttgctcaac gagcggctgc tagatcctct cgatggtctg acgaaccgat gcgtgcaaac
     3361 gatattgaca gttcctgagg tatccgcttc cttcaaggaa cgcttggcac cgtgggcgtt
     3421 tgcgctgttc aagcatggtt ccaacgtgca tggggaacgg ggatgcgtgc tcgtgcctcc
     3481 cgcgtctcgt gagcaatttg aaaaacagat tcagggtcta tcggcgcggc aacgggggca
     3541 ataccggtgg cgcgattttc tcgatcatat ttatgacgcc acacctctcg tgactcaggg
     3601 cttttacctt ttcgaccgtc cggaagatcg gctgcgattc gaagaagcgt tgggctctga
     3661 atacgccctg attcgaaaga agctccatag gattttcaat gatgcactgg ggaacgacgg
     3721 tgccataccc gcgcggcaat ggaagcgttt cgatgagttg agccagcaag ctctttcgat
     3781 gcagcaaacg tttctcgaac ccgcgctgtc ggcagccgag ggcgaggtgc gagcgatcgc
     3841 gacgcgggtg aggattcgct gttatgaagg gggggaactt cgctttttgg ttcgcccgta
     3901 tagctttgaa tttgcagaac aattcgatga aatggccgag cttctggagc gtgttcggat
     3961 gcaggttcct ccccacgcca tcgaattgcg ggaggtgctt gcggatttat ccgattggtg
     4021 tcgggcgcga acgaccgatg agtcttgggc agagtgcttg ccgagttgga ttgccgcgtc
     4081 ggatccaacg aacttgatcg atgtgaactt gacggtggaa gagaaggtat cgcggcttgg
     4141 cgcgaaaggc ggaattcaac tcgtcgtctc tgcctttgaa accgttccgg agccgcttcg
     4201 tcctattgtt tccatgctga acgattccaa ccaaggcgcg aatctcaacg tggtttggct
     4261 caagcatttg ctagtcggcg gcggtgcaag caacatgacc atggccggcg aaaagcttcc
     4321 ggattcagaa ggacgcgatt gctacaaggt gatgaccttc accaatgccg tacgctcctc
     4381 gatggtggaa agccaggcgg aactcatggt ggcgtcgacc cacttttccg aagacgacat
     4441 acctcgtctt gcgcacgcgg caaacctgct cgtgctattg catgaggttg ggcatacgtt
     4501 cggggacttc gccgagtttc ttggagagta tggggccagt gtggaggaga ctcatgcgga
     4561 agccagcgtg atatttctcg cgcatcgatg ggcgccgcaa tcccttgtcg acctcattgg
     4621 tttgacggct tgttggacgc cggtgcatcg gtgcttgcaa ggagcggcgg aagcccacag
     4681 ccattccgat atcgttctat tcgatgagat attgcgcgcc gggggagttg cgtgtgtcga
     4741 gcgtgatggc agaccgatcg ttgcccccat gtctgctgat gaagcggtac gtgcggcctt
     4801 tgatctttct ttgcggatgc gcctttggga gcttgggatt ccttctggcg agcacggtac
     4861 gtggtgggta cgtttcgatc cgaacgatcc cgcactggat gttcgggtga tacaacgagc
     4921 gcgcaaggcg gtcgaactgc tggggcagga gcaacgtcgc gcgtggcaaa cccaagtatt
     4981 cgaggaatgc aaagtattct tcgcacaaag cagactcgag gagctttcgc gccccttgca
     5041 gccgatcatc gagcgtttgc ctcgttacca accgctgtcg atcattccca ccgatgaacg
     5101 ctttcggctg gtggcttcct tcgtctaacg acgtgtagca cccatggcca cctggaagga
     5161 tgcggagaaa tgagatagcc cagcaactgt aaccatgtaa attaaaagac atttttagaa
     5221 tcccccccgc ctcgtggatt cacgttggaa aaggtccccc cttgcggtgg gtggtgtttc
     5281 gcataacgtt tggccgcacg actcgttcat atttgcgtgg gcgaaacatt tgccttcaca
     5341 ttgctctcac tcaactcttg cctgactcgt tttccagggc tagataagga gtgctcgcga
     5401 tgagggagat cgcgattgat atagaaagcg gtcgacaaca tctcgaatct agcaggaggc
     5461 cttcatggca aggaagatca cggaagagtg catcaactgc ggagcttgtg agcccgaatg
     5521 ccccaacgag gcaatcagcg caggcgacga catctacatc atcgaccctg acaagtgcga
     5581 cgagtgcgaa ggccgcgaaa cggtaggctg tgtcgaagtc tgcccggtcg cggatgaagc
     5641 tatcgtcatg gcttgattgg gttcacactt ctttcacgac gtgcccggag agaacgattt
     5701 tctccgcgca cgtcgtttgt ttttccatct catgatacga tcggcgcgtg tcgatgatac
     5761 ccacgcggtt cgccaagcaa gcttggtctg cctttgccgt tcccatggtt ttttcgctgt
     5821 attcctgtgt ggaaaccagc ggctcggcat ggatgcggca gcctcttact cccgatgacg
     5881 aaccatcttc ggtgatggtg gagcctcgct atcgaggaag cggaacgaga aaggatacgc
     5941 gcacgcatac ccttgga
//
LOCUS       DAOSOR010000363         5945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13510_length_5945_cov_5.002547, whole genome shotgun sequence.
ACCESSION   DAOSOR010000363 DAOSOR010000000
VERSION     DAOSOR010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13510_length_5945_cov_5.002547"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..4705
                     /locus_tag="PK710_17230"
     CDS             17..4705
                     /locus_tag="PK710_17230"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT11524.1"
                     /translation="MAAETAARSEASRDRAIELYRLLIEDRPDDAKAISNLTRLYSGA
                     DRTKDLLELRRHELGLAEDLDRRISLRLQIASLLVTLGDATDAITTLRDGLEEKADHE
                     PTIDALIVQYEEQKRFDDLAEMLKTQAAAVQALGRTELAVRLWLQAARVCKTQLLDLE
                     EAVQCYQRVVDLQPNIEALDELASACLQHEQYATAVSWLQQRLAISPSGQRTPTVERL
                     ATAQLGAKQTSQALETLRSGLAEDPGSLALRDKLAELYRTAEAWSELVVVLTEGADGS
                     DDEAVRKRYLLEASDVLLHGLRSAHEAVPVLEQLVKLQPEDRAVRFTLATALFQAERF
                     EQARTLTTKLIDEYGRRRPPERANLHLLLAQILRAQDKPTEAIQQLEQGVAMDMGNLR
                     LQHLLGQVYRQTGQLDKAERAYHALVLLLRRRAALQRGPLQEGDVGIAEVLFELHLVS
                     KDLGAQERAVENLESAFDAAALDPSENERFETALRAFGSLELLLRSLQQRLAIVDTAV
                     QRANVLAEIAEAQGKLGQSDLALASLIQAIEENPEATALYDKAQELAQRAGELDEYAA
                     MLEKASDRALQDRDQQLACRLLMQLGNLEEQERKNLNRAAQLYARAQQTECALPMVWS
                     ARARVAALVGDTTTELEMLHLLIDLPSDEVSKERTDALYRLASLELANRDSMQSGVIT
                     LTTALQTEPRYAQASLALQQALALNPECEEAIIAFERVARAANDDRWLLEALERRLHV
                     QAGTQPMLQEAVDVAQRIGDTEKLEAFLLRSIDLAKQDDGTLVPALWAMQALADLRES
                     QGNLKDAMQWLQQAAEVASDDEARALSLRVAQIAADSLNELETAVLAYERLLRLDPGD
                     PNVWQPLLAVVRRMGDREKLEATLRSIIDDVSDLALRSGLRIELASLVRDKDRRDEAI
                     ELLRTVLAEDAEHAHAAEELGDLLQDSGRADELVALLTQQLEVSRLRSDSLSGSQFAR
                     RLTDLVADSNREQALQVFRDTLAWIPDDGQLMRGLLALLDPDLDAQERAEVIEKLLNT
                     EGADDPLSLALDLVDARARMQDEAGLERALESAYRLEPEEPRVLEQYRRLAEQLENEA
                     QDLSDTEQAVKKLFRAASIHADRLDDPGTAGQLLAAAQNLQPSDLGIIEQLVQAMMQS
                     DQLPVAIERTTEAIERWDGDEAGRAQLHRLRASIWELSGNHEAAADDVEYACRIEGQS
                     SMPELLDALTKARDAAVERTDFDAERNATLRIITLLSEMGQPEEAREALNRWVSSAPE
                     DLQALRMLIEIDRAAGRWQDVAANCEHLVNNDTGISLAEGALMHAEACDHLGNPELAR
                     ASLERALQHDNSHEEVRLRLRTLYEQAGEYGPLSNLLLIDANLAQDDGTRFEFLREAG
                     RMRLANFDQAASAIGPLSQALELQPNDLEVMLLLADAYISAGLIQEVVQLLQEGIDRQ
                     GGRRSRELAALQHRMARAAATSDRNTEMQWLLAAFESYPQGTDVASELADLAMALGDY
                     EIALKALRTLATAKTQGPISRPMAFLKQAQIAHMQGDDRKASFLAKKAVAMDPEFIEA
                     RDFLDQIGGG"
     gene            complement(4944..5312)
                     /locus_tag="PK710_17235"
     CDS             complement(4944..5312)
                     /locus_tag="PK710_17235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmO family methyltransferase"
                     /protein_id="HOT11525.1"
                     /translation="MLSVDVKPIGVIRNRGDGIAMLSVFAAYRQGLEGLEVGARVQVL
                     YWMHELTHGDRRSLRVHPGGDPDREMKGVFSLRSCVRPNPIGVTQGQVVMVNDDGLSV
                     SGLDAKDGSPLIDIKASCSE"
     gene            complement(5360..>5945)
                     /locus_tag="PK710_17240"
     CDS             complement(5360..>5945)
                     /locus_tag="PK710_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT11526.1"
                     /translation="AEYKSFSHPDIVLSRTSIRQGQARSIGMPDKSKEALERAANRWH
                     HVHALALLAPTIGLTGPIARYTAYEDTGGPFYLDTNGSTVDPILDDMGLWLKTRHGLV
                     VMTGCGHAGIVNTVRQAIDQSGETRIHAVIGGFHLGEANEERLDRTVEALQAWSPDLV
                     VPCHCTGEAAVARLSDMPGDRVVVGHAGMKLDMT"
BASE COUNT         1322 a         1734 c         1681 g         1208 t
ORIGIN      
        1 atatcggcta ttgcacttgg ctgccgaaac tgccgcccgt tcggaggcca gccgcgatcg
       61 tgccatcgag ttgtatcgac tattgatcga ggatcggcca gatgacgcca aagcgatttc
      121 gaatctcacg aggctttatt ccggagcgga tcggaccaaa gacctgctcg agcttcgtcg
      181 tcatgagcta ggtcttgcgg aggatctcga caggaggatt tctcttcgtt tgcagatcgc
      241 ctcgctgctg gtgacccttg gcgatgcgac cgacgcgatc accacgttgc gtgacggctt
      301 ggaggagaaa gccgaccatg agccaaccat cgacgcgttg atcgtgcagt acgaggaaca
      361 gaagcgtttc gatgacttgg cggagatgtt gaaaacacaa gccgcagcgg tgcaggctct
      421 cggtcgtacc gaacttgctg ttcggctttg gttgcaagcg gcacgagtct gcaagacaca
      481 attgctcgat ctcgaagaag ctgtgcagtg ttaccagcga gtcgtcgatt tgcagccgaa
      541 tatcgaagcg ttggacgaac ttgcctcggc ctgtctgcag cacgagcaat atgcgaccgc
      601 ggtatcatgg ttgcagcagc gtttggcaat tagtccgtca ggtcagcgaa cgcccacggt
      661 agagcgtctt gccaccgcgc agctaggagc gaagcagaca tcccaagctc tcgagacgct
      721 tcgaagcgga ttggcggaag acccggggtc tcttgcgctt cgggacaaac tcgcggagct
      781 atatcgcacc gctgaagcct ggtcggagct tgtggtcgtg ttgaccgaag gggccgatgg
      841 tagcgacgat gaagccgttc gaaagcggta tttgctcgaa gcctccgacg ttttgcttca
      901 tggtttgcgc tcggcgcatg aagcagtgcc ggtgttggag cagctcgtca agcttcaacc
      961 agaggatcgg gcggtgcgtt ttacccttgc caccgcgctg ttccaagcgg aacgattcga
     1021 gcaagcgcgc acactgacca cgaagctcat cgacgaatat gggcgtcgtc gtccacccga
     1081 gagggcgaat ctccacttgc tgttggcgca gattttgcgc gcccaagaca aacccacgga
     1141 agccattcag cagttggagc aaggggttgc catggatatg ggcaacctcc gtttgcagca
     1201 tcttctcggc caagtgtatc ggcagacggg gcaactcgat aaggcggagc gagcgtatca
     1261 tgcgctagtg ctacttcttc gacgtcgagc cgcactgcaa cgcgggcctc ttcaagaagg
     1321 cgatgttggc attgccgaag ttcttttcga attgcacttg gtgtccaaag acctgggagc
     1381 ccaagagcga gcggtggaaa acctcgaatc ggcgtttgac gccgcggcgc tcgatccttc
     1441 cgagaacgag cgattcgaaa cggcgttgcg cgcctttggc agcctcgaac tgctcttgcg
     1501 ctcgctacaa caacgtcttg ccatcgtgga tactgccgtg cagcgtgcca acgtgcttgc
     1561 ggaaatcgcg gaagcgcagg gcaagctcgg acaatccgac ctcgcgttgg catcgttgat
     1621 ccaggctatc gaagaaaatc ctgaagccac cgcgttatat gacaaggccc aagagttggc
     1681 gcaacgagcg ggagaactcg atgagtatgc agccatgctc gaaaaagcct cggaccgagc
     1741 gttacaagac cgggaccaac agcttgcctg tcgcttgttg atgcaactcg gcaatctcga
     1801 ggagcaggag cggaaaaacc tgaatcgtgc cgcgcagtta tacgctcgag cccagcaaac
     1861 ggaatgtgcc ttgccgatgg tctggagtgc tcgtgcccgt gtcgctgccc ttgtgggtga
     1921 cacgaccacc gagttggaga tgctgcatct gctcatcgac ttgcccagcg acgaggtgtc
     1981 gaaggagcga acggatgcgt tgtatcgctt ggccagtctc gaactagcca atcgcgattc
     2041 gatgcaatcc ggtgtcatca ccctcacgac ggcgttgcaa acggagcccc gatacgcaca
     2101 ggcatcgctc gctcttcaac aagcgcttgc tctgaacccg gaatgcgaag aagccatcat
     2161 cgctttcgaa cgcgtggcac gagcggccaa cgacgaccgg tggctgctcg aagccctgga
     2221 gcgacggctt catgttcagg cggggacgca gcccatgttg caggaagcgg tcgacgtcgc
     2281 ccaacggatt ggggataccg aaaaattgga ggcgtttttg ctgcgctcca ttgacttggc
     2341 caaacaggac gatggcacgc ttgtccctgc tctttgggca atgcaagcgc tcgctgactt
     2401 gcgcgagtcc cagggcaact tgaaagatgc catgcaatgg ctgcagcaag cggcagaggt
     2461 tgcatccgac gacgaagcgc gagccttgtc gctgcgagtt gcgcaaatcg ccgcggattc
     2521 gttgaatgaa ctcgaaaccg cggtactggc ttacgagcgc cttttgcgct tggaccccgg
     2581 cgatccgaac gtttggcaac ctttgcttgc cgtggttcgg cgcatggggg atcgtgaaaa
     2641 attggaggcg actcttcgtt ccatcatcga tgacgtttcc gatctcgcgc ttcgcagcgg
     2701 gcttcgcatc gaactggctt cgctggttcg cgacaaagac cgtcgtgatg aggccatcga
     2761 actgttgcgc acggtcttgg cggaagatgc ggaacacgcc catgctgccg aggagcttgg
     2821 tgatctgctg caagacagtg gcagagccga tgagttggtt gccttgctga cacaacagct
     2881 cgaagtcagt cgcttgcgtt cggactctct ttccggaagc cagttcgcac gtcggttgac
     2941 cgatttggta gcagactcga accgcgagca agccttacag gttttccgcg atactcttgc
     3001 atggatccct gacgacgggc agctcatgcg cggcctgcta gcattgttgg atccggactt
     3061 ggatgcgcag gagcgcgcgg aagtcattga gaagctgttg aataccgaag gggcggacga
     3121 tccgttgtcg ctggcgcttg acttggtgga tgcgcgagcg cgtatgcaag acgaagctgg
     3181 actcgagcgc gccttggaat ccgcgtatcg tctcgaaccg gaggaaccac gagttctcga
     3241 acaatatcga agacttgcag aacaactgga gaatgaagcc caggatttat ccgacaccga
     3301 acaagccgtg aaaaaactgt ttcgagccgc gtcgattcac gccgatcgcc tcgacgatcc
     3361 gggcacggcg ggacagctac tcgcggccgc gcaaaacctt caaccaagcg accttggtat
     3421 catcgaacaa ctcgtacaag ccatgatgca aagcgatcaa ttgcctgtcg ccatcgaacg
     3481 aacgaccgag gcgatcgagc gatgggatgg cgacgaggcg ggaagggccc agttgcaccg
     3541 gttgcgtgca agcatttggg aattgtcggg caaccacgaa gccgcggctg acgacgtgga
     3601 gtatgcttgc cgaatcgaag gtcagagttc gatgcccgaa cttctggatg ctttgaccaa
     3661 agcccgcgat gcggcggtgg agcggacgga tttcgacgcg gagcgaaacg cgacactgcg
     3721 tatcatcacg ttgttgagcg aaatgggaca gcctgaggaa gccagggaag cgctgaaccg
     3781 ttgggtatcg agtgctcctg aagacttgca ggctcttcgc atgctcatcg aaatcgaccg
     3841 cgcagcaggc cgttggcagg atgtggcagc caactgtgag catctcgtca acaacgacac
     3901 cgggatttca ctagccgaag gcgcgctgat gcatgcagaa gcatgtgacc accttggaaa
     3961 tcccgagctt gcaagagcga gcctcgagcg tgccttgcaa cacgacaact cccacgaaga
     4021 agtgcgcctt cgtttgcgaa cgctctatga acaagccgga gagtacgggc ccttgtccaa
     4081 ccttcttctg atcgacgcca acttggcgca ggacgatggc actcgattcg aattccttcg
     4141 tgaagccggg aggatgcgcc tcgccaactt cgatcaggct gcttccgcca ttggcccatt
     4201 gtcacaagcg ttggagttgc aacccaacga cctcgaagtc atgcttttgc tagctgacgc
     4261 ctatatttcc gcgggcctca ttcaagaggt cgttcaactt cttcaagagg gaatcgaccg
     4321 gcagggagga cgtcgctccc gtgagttggc ggcactccag caccgcatgg cacgagcggc
     4381 agcaacgagc gatcgaaata cggaaatgca gtggctcctg gcggcattcg aaagctatcc
     4441 gcagggaacg gacgtggcat cggaactcgc ggacctggcc atggctttgg gagattacga
     4501 aatcgccctg aaagcattgc gaactcttgc caccgccaaa acgcaaggtc ccatctccag
     4561 accgatggcc tttctcaaac aagctcagat tgctcatatg caaggcgatg atcgcaaggc
     4621 cagcttcctc gccaaaaaag ccgtcgccat ggatcccgag ttcatcgaag cacgcgactt
     4681 tctcgatcag atcggtggcg gataatggct tgacccaccc tagatccccg tcgaacgatt
     4741 cctgctaccc ttcacttcca cgatcacccg ccaaaacgct tcacccttca tcccatgagc
     4801 gacgaccggt caccatccag cggcgatcac gtatggatga gccaaccgat gcatccaaaa
     4861 aatcctcgaa actcatcgat tggcgcaacg taccacggaa ccacctccaa gccgcgaatc
     4921 aatgcccatt gctgtctttc ccttcactcc gaacaactgg ccttgatatc gatgagtgga
     4981 gaaccatcct tcgcgtccag acccgaaacg gacagcccat cgtcgttcac catcaccacc
     5041 tgcccttgtg tcacgcctat cggattgggt cgcacacagc tcctaagaga aaaaactcct
     5101 ttcatctctc gatcgggatc ccctcccgga tgcacacgca aagacctacg gtccccatgc
     5161 gtcaattcat gcatccaata aagcacttgg acgcgggccc ccacctccaa tccctccaac
     5221 ccttgccgat atgccgcaaa cacgctcagc atggcgatgc catcaccacg attccgaatc
     5281 acaccaatcg gcttcacatc cacactgagc atgacggacc ttcctttcgc tatcccgtcg
     5341 tttcaccacc gatttcgaat caggtcatat caagcttcat gcctgcatga ccgaccacca
     5401 cacggtcacc tggcatgtcg ctcaaccgtg ccacggccgc ttccccagtg cagtggcaag
     5461 gcacgacgag atccggcgac caagcctgta acgcttccac ggttcgatcg agtcgttcct
     5521 cgttggcttc gcccaagtga aatccgccaa tcaccgcgtg gatacgtgtt tcgccgcttt
     5581 gatcgatggc ttgccgaacg gtgttcacga tccctgcatg accacatccg gtcatcacta
     5641 ccaagccatg tcgggttttc aaccataacc ccatgtcatc caagatgggg tcgacggtcg
     5701 acccattcgt atctaaataa aacggtcccc ccgtgtcttc gtaggccgtg tatcgtgcaa
     5761 tcggacccgt caatccaatg gttggtgcca acagcgcgag ggcatggacg tggtgccaac
     5821 ggtttgccgc acgctctagc gcttctttgg atttgtcggg cataccaatc gaacgagcct
     5881 gaccttgccg tatcgacgtt cgcgaaagca caatgtcggg gtgtgaaaaa cttttatatt
     5941 ccgcg
//
LOCUS       DAOSOR010000364         5942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13524_length_5942_cov_4.372176, whole genome shotgun sequence.
ACCESSION   DAOSOR010000364 DAOSOR010000000
VERSION     DAOSOR010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13524_length_5942_cov_4.372176"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1215)
                     /gene="rsgA"
                     /locus_tag="PK710_17245"
     CDS             complement(133..1215)
                     /gene="rsgA"
                     /locus_tag="PK710_17245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOT11527.1"
                     /translation="MAANEIALSTSLEDLGWSAFFEQQRTHVDSHLTLGRVCLSHREH
                     YTLLTTHNPLQARLGGRLRHRATGPLDLPSVGDWVAATPASDGHAAAVIHVFARRTQF
                     LRKVVGRRTEVQVLAANINHVAVVTSPNDDFNPNRLERYRTAIRQGGANVLFVLNKAD
                     LRNDLDGYLEAFAKIDPSATVLVTRALEGQGVDALRSYCATGETLALVGSSGVGKSTL
                     VNAMLGRPVQRVGKTRETDGRGRHVTSHRELFLLEDGGLLMDSPGMRELQVWSSDPQQ
                     DTFWDIASLANSCRFRDCTHQHEPGCAVQRAVDTGELDAQRLEAFHRLQQENLQLEER
                     RQDAISSKSKHLSFHRSLKNDDEQDG"
     gene            1307..1882
                     /locus_tag="PK710_17250"
     CDS             1307..1882
                     /locus_tag="PK710_17250"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HOT11528.1"
                     /translation="MVRSHEKKMVADKRGSLDRGSMVHPARSRWSLTPLAIVGVLIGC
                     ASTPPASTPAPEPFEPEPEAPVAPPEPKPEPEAGRVELSTAFLVGGDMPSSTRDQVTA
                     QVVQAINDASSSFRKCYAKAVARGHSPKGEIDIELLIRPGGSIGALRPGKGTLTEEEL
                     VACTVRVFERLEWPSPPADYSIIAPIQYYPE"
     gene            complement(1990..4665)
                     /locus_tag="PK710_17255"
     CDS             complement(1990..4665)
                     /locus_tag="PK710_17255"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT11529.1"
                     /translation="MNVLRGRIVVGICPQCGRARETSYRFCPQCGFPVEHIEASPNDP
                     LIGRVLGQAYVLLEKVGTGGMGAVYRAEQRTLGRTVAVKVIHAHLLHDPGVGDRFFNE
                     AHAASRLSHPHLVSVIDFGKTEDGLPYLVMEYLRGRDLRSLLQEEGPLELSRALTIFQ
                     QVLEALAEAHALGVVHRDLKPANIVVEQTRAGCDFVKVVDFGLAKIIHGSSERALVTG
                     DLVRGTPCYMAPEQAMGGAIDPRTDIYAAGVTLFELLTGRPPFEARSPMELMLDHLNT
                     APPDPRTLAPSRHLPAALVTACLRALSKKPDERYQSAEDFLRDLRGSDMSLDEAGAYP
                     TIPVPGRAASCPSCGEPLTSSQKFCGACGERIPSGPPLSGPQSPSRPRLPDDPQSLFA
                     PQDNAHLLWLQQLRLSHTTGTKAVRLVGPHGSGKTSILRDVAAFARMQGDAVGCMEPD
                     PWWAEPSEYSLRHLILQLVRLPEDGKDPLSWAGAWPEAVLGLQTLFLTTNASSPHSPE
                     TLRAAACAALQWAIERAAQSALSGHVLVLADDLERIDGVSRNALADALALPIRVPWLL
                     MVTHVPRQDIGWRYDLPERVLQGIPVESARHTLRAIGARDDVFEGQSHVLPLLVEQRV
                     RAEIEGHNLPPMGLADVISYRMAALPLHARMVLDALGILGDDVPMEHLNQVVDHSCDV
                     NEGLRALAVSGFIDRRSHGLCWVNPLFREVAKARIPAALRIALSERAADVCHENDRPV
                     EARAAFALEAGRGLEALFLLEYVARSAKSHGNDEGAARALKQGVDLARQTPPAKGIDQ
                     PQHAAVLFGRKLGETLLDLGQLAQSRQVLQQALELADGDSEVDVLRALARVARAENRS
                     GEAQAFLKEAITRAKQTGRHEVLDSLVELERKWAS"
     gene            complement(4734..>5942)
                     /locus_tag="PK710_17260"
     CDS             complement(4734..>5942)
                     /locus_tag="PK710_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11530.1"
                     /translation="AGPRALVDSDWLARQVAHVDNTQGDIDTLMLRLAFIRTWTYVSN
                     HDAWVDEPRAWQQHTRQIEDRLSDALHERLIQRFVTQKRPRLYSPATPRARSSPNNED
                     EHPNKKKGPFQTLAAWKARLATATQVAAPSHGIDDVLESDFESFQVDALGQILLDGLC
                     VGRLSRGIDLNRPDVAVVAEDSLRAGPKLQIQRRLQAFARSWAQQVIAPLQGPCLESL
                     TPAGRGLLHLLSARLGTVAMSVAREQVALLPPSDRNTLKNMGIVLGSRFVFSRPMLDP
                     QIVPRRVLLCRIFLSFPGVGIFPDGHSTYCSLQPGVDPSAYTAMGYPVLGDRGIRVDM
                     VDRVLGVLRNHRESDRFDVPERIVAWLGLEPGADIQALKLAFSIAPSKRNADANVRKP
                     KPRTKSKPYK"
BASE COUNT         1246 a         1581 c         1873 g         1242 t
ORIGIN      
        1 gcttcgacgg agccataggt ccatgaactc gttcgagcgt cgtcaccaca tcccatgctt
       61 aggaaggctg cgactagcaa agagagttgg gagcgcatgg ccatatcatg gctggatggc
      121 aagccaggcg catcatccgt cttgctcatc gtcatttttc aacgagcgat gaaaagataa
      181 atgctttgat ttcgatgaga tagcatcttg acgtcgctct tcgagctgga ggttttcctg
      241 ctggagccgg tgaaatgcct ccaaccgttg tgcatctaat tcgcccgtat cgacggctcg
      301 ttggacagcg catcctggct catgctggtg cgtgcaatca cgaaagcggc atgaattcgc
      361 caaggaggcg atatcccaga acgtgtcttg ctgcggatcc gaactccaca cttgcaattc
      421 tcgcatccct ggggagtcca tcagcaatcc tccatcctcg agaagaaata actcacgatg
      481 ggaagtcaca tggcgtcccc ggccatccgt ttcgcgtgtc tttccaacgc gttggacagg
      541 acgtccgagc atggcgttga cgagggtgga ctttccgacg cccgacgagc ctaccagcgc
      601 caaggtttcg cccgtggcgc agtaggatcg taatgcatcg acaccttgtc cttccagcgc
      661 ccgcgtcacg aggacggttg cgctgggatc gatctttgcg aaagcttcga gataaccgtc
      721 gaggtcgtta cgcaagtccg ctttgttcaa gacgaagagg acgttggcgc ccccttgccg
      781 gatagctgtc cggtaccgtt cgagtcgatt cggattgaag tcatcgttgg ggcttgtgac
      841 aaccgcgaca tggttgatat tggcggcgag cacttgtacc tcggtgcgtc gtcccacgac
      901 ctttcgcagg aattgggttc ggcgcgcgaa gacgtggatc acggcggcgg catggccgtc
      961 ggaagcgggt gttgcggcta cccaatcgcc gacagaaggg agatcgaggg gacccgtggc
     1021 tcgatgacgg agacgcccac caaggcgagc ttgcaaagga ttgtgggtag tcagcagcgt
     1081 atagtgctca cgatgggaga ggcaaacgcg ccccagggtc agatgcgaat cgacatgggt
     1141 gcgttgctgt tcgaagaacg cggaccatcc caggtcttcc aacgatgtcg aaagcgcgat
     1201 ttcgttcgcg gccatgggtc catggctacg cttgcttggg cacggtggca agaccgcgga
     1261 caagtcgttc gacatcctca atggggagca ctaggggaaa aacgtcgtgg tgcggagcca
     1321 tgaaaagaaa atggttgccg acaagcgcgg ttcgttggac cgtggctcca tggttcatcc
     1381 tgcacgttcg aggtggagtc tgaccccgtt ggccatcgtg ggcgttttga ttggttgtgc
     1441 gagcacgccg ccagcttcta cgccggcccc tgaaccgttc gagccggagc cggaagcacc
     1501 cgttgccccc ccagaaccga agccggagcc cgaagccgga cgcgtcgaat tgagcaccgc
     1561 ctttctcgtg ggaggagata tgccgtcatc gacgcgagat caggtgacgg cacaggtcgt
     1621 tcaagcgatt aacgatgctt cgtcttcgtt tcggaagtgt tatgcgaaag ccgtggcgcg
     1681 aggacattct cccaaggggg aaatcgatat cgaattgctt atccggccgg ggggaagcat
     1741 tggagcgctt cgacctggca agggaacact tacggaagag gagttggtgg cgtgtacggt
     1801 tcgcgtattc gaacggctcg agtggccatc accgcctgcg gattattcga tcatcgcacc
     1861 gatccaatac tatccggagt gattggtgcc atgacgtgcg ccaagggatg aggcagagga
     1921 gatgggggga tcaagggggc gtgtctatct acttgaattc attgaatatt tcaccatatc
     1981 aaccagatct taggaggccc attttcgttc gagttcgacg agcgaatcga ggacttcgtg
     2041 gcggccggtt tgtttggcgc gggtgatggc ttctttgaga aaagcttgag cttccccgga
     2101 tcgattttcg gctctggcga ctctggccaa ggctctcagt acgtcgacct cgctatcgcc
     2161 atccgccaat tcgagggctt gttgcaggac ttgtcgagac tgcgcgagtt ggccgagatc
     2221 gagcagggtt tcgcctaatt ttcttccgaa tagcacggcg gcgtgttggg gttggtcgat
     2281 gcctttggct ggcggcgttt gccgagcgag gtcgacgcct tgtttcaggg ctcgagccgc
     2341 gccttcatcg ttgccatggg attttgcgga gcgagcgaca tactcaagca aaaacagcgc
     2401 ttcgagacct cgtcccgctt cgagggcgaa ggcggcgcgt gcttccacgg gacggtcatt
     2461 ctcgtggcaa acgtcggcgg cccgttcgga gagggcgatc cgtaatgcgg caggaattcg
     2521 ggcttttgcc acttctcgga acaatgggtt gacccagcac aatccatggc tgcgacgatc
     2581 gatgaagccg ctgacagcga gggctctaag gccttcgttc acatcacaag agtggtccac
     2641 cacttggttg aggtgttcca tgggcacatc gtcgcccagg atgccgaggg catcgagcac
     2701 catgcgagcg tgcaggggaa gagcggccat tcggtaggag atcacgtctg ccaggcccat
     2761 gggaggcagg ttatggcctt cgatttcggc tcgtacgcgt tgttccacga gcaagggaag
     2821 cacgtgggat tggccttcga acacatcatc gcgggcgccg atggcccgga gcgtatggcg
     2881 ggcgctttcc accgggattc cttgaaggac gcgttccggc aggtcatagc gccatccgat
     2941 atcttggcgc gggacatggg taaccatcaa cagccaaggg acgcgaatag ggagagccag
     3001 agcgtcggcg agggcgttgc gcgagacgcc atcgatgcgt tcgaggtcat cggccaagac
     3061 caggacatgc ccgctcaagg cgctttgtgc ggcgcgttcg atggcccatt gcagagctgc
     3121 gcaggcagcg gcccgaagcg tttccgggga gtggggggag gaggcattcg tggtgagaaa
     3181 gagggtttgc aatcccaaca cggcttcggg ccaggcgcct gcccaggaaa gcgggtcttt
     3241 accatcttct ggcaagcgga cgagttggag gatgagatga cgcagggaat attccgatgg
     3301 ttcggcccac cacgggtcag gttccatgca gccgacggca tccccttgca ttcgggcaaa
     3361 cgcggcaaca tctcgaagga tcgaagtttt tccgcttccg tgtggaccga cgaggcgaac
     3421 ggctttggtg cctgtggtgt gggatagacg caattgttgg agccacagca gatgagcgtt
     3481 gtcttgggga gcgaaaagcg attgcgggtc gtcagggagt cggggtctgc tggggctttg
     3541 cggtcccgac aggggggggc ccgatgggat acgttcaccg caggctccac agaatttctg
     3601 cgatgaggta aggggttctc cgcaagaggg gcaagaggcg gccctgccgg gcacggggat
     3661 ggttggatag gcaccggctt catccaggga catatccgac ccacgcaggt cacgaaggaa
     3721 gtcctcggcg gattgataac gttcatcggg tttcttggaa agcgcgcgaa ggcaggcagt
     3781 cacgagagcg gcgggcaagt gacgagacgg cgccagcgtt cgaggatcgg gaggggcggt
     3841 attgaggtgg tcgagcatca gttccatggg tgatcgcgct tcgaaaggcg ggcggccggt
     3901 gagaagttcg aatagcgtga cgccagccgc atatatatcc gttcgcggat cgatggcccc
     3961 tcccatggct tgctcaggtg ccatatagca aggggttccg cgaacgaggt cgccggtgac
     4021 aagggcgcgt tcggagcttc cgtggatgat tttggccaag ccaaagtcga cgaccttgac
     4081 gaagtcacaa cccgcccgcg tttgctcgac cacgatattc gcgggtttca ggtctcgatg
     4141 aacgacgccc agcgcatgcg cttctgccaa ggcttccagc acttgttgaa agatggtgag
     4201 cgcacgcgac agttcgagag ggccttcttc ttggagaagg ctcctgaggt cacgacctcg
     4261 aaggtattcc atcaccaggt agggaagacc atcttcggtt tttccaaagt cgatcacgct
     4321 aacgaggtgg ggatggctca accggctggc cgcgtgagct tcgttgaaga agcgatcgcc
     4381 cacaccaggg tcatgtaaca aatgggcatg aatgactttg acagccaccg tacgccctag
     4441 ggttcgctgc tccgctcggt acacggctcc cattccgccg gtgcctactt tttccagcag
     4501 cacgtaggct tgaccgagca cacggccgat aagcgggtcg ttgggggaag cctcgatatg
     4561 ctcaacgggg aaaccacatt gtgggcagaa acgatagctc gtttcgcgcg ctcttccaca
     4621 ttgagggcaa atcccgacca cgattctgcc ccgtaacacg ttcattgaaa cttgacaact
     4681 caagcataag ggctacggca tcatttttcc gaacgatcat tgttcgaaca tcgttattta
     4741 taaggtttcg atttcgtcct tggcttcggc ttcctgacgt ttgcatcggc atttcgtttt
     4801 gatggggcga tagagaaagc caatttcaac gcctgaatgt ccgctcccgg ctccaaaccc
     4861 aaccaagcaa caatgcgttc cggaacatcg aaacggtcag attctcgatg atttctgagt
     4921 actcccagca cgcgatccac catatccacc cgaattccgc gatctcctag gaccggataa
     4981 cccattgccg tatacgcgga gggatcgaca ccaggttgca acgaacagta ggtggagtgg
     5041 ccatcgggaa atataccgac tcctggaaat gaaagaaata ttcgacataa caagacacgt
     5101 cgtggaacaa tttgtgggtc gagcatcggg cggctgaata caaacctaga accgagaaca
     5161 attcccatgt ttttcagggt atttcgatcg gaagggggta gaagggccac ttgttctcgc
     5221 gccacggaca tggcgacggt gccgagacgt gccgagagca aatgcagcaa gccacgtcct
     5281 gctggggtca aggattccaa acaggggcct tgaagcgggg cgatgacttg ttgtgcccaa
     5341 cttctcgcaa acgcttgtaa tcgtcgttgg atttgcagtt tgggaccagc tcgcagggag
     5401 tcttctgcga cgacggcgac atcggggcgg ttgaggtcga tgccgcgaga caggcgtccg
     5461 acacacagtc cgtcgagcaa gatttgaccg agggcatcga cttgaaaaga ctcgaaatcc
     5521 gactcgagga catcgtcgat cccatgggaa ggagcagcga cctgcgtggc agtggcaagg
     5581 cgggctttcc atgcggccaa tgtctggaag ggtcctttct tcttgtttgg atgctcatct
     5641 tcgttgtttg gcgacgagcg cgcgcggggg gtcgcagggg aatacagtcg tgggcgcttc
     5701 tgcgtgacaa aacgttgaat gagccgctca tgcagagcat cgctcaaccg gtcttcgatc
     5761 tgtcgcgtat gttgctgcca ggctcgcggc tcatcgaccc acgcgtcatg gttcgagacg
     5821 taagtccatg tacggatgaa cgcgagacga agcatcaagg tatcgatatc cccttgtgtg
     5881 ttgtcgacat gggcgacctg acgcgcgagc caatccgaat ccaccagagc gcgtggtccc
     5941 gc
//
LOCUS       DAOSOR010000365         5929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13562_length_5929_cov_4.412836, whole genome shotgun sequence.
ACCESSION   DAOSOR010000365 DAOSOR010000000
VERSION     DAOSOR010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13562_length_5929_cov_4.412836"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="PK710_17265"
     CDS             complement(<1..1098)
                     /locus_tag="PK710_17265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT11531.1"
                     /translation="MSERFRVEQESPAESMRRPDEHPWAPVLEKAFDACPHPLVILHR
                     TDHTILEINQAMSRVTGFSRDQIVGRTLTDIELFKGKAEGQSIDDVLSFFENSVANHP
                     VRLHAVAGNTIECLLSAAPFHANDEPYVVMSFEDSHDRLDVEKALEVTQQRLELAFEG
                     SSTALWEWSLPDDTGFSIAYYESMLGYPEGSLARGLNQVRALIHPEDVEAAIQALREH
                     VKGAATSWSEEFRVMTRAGRWIWVHASGRIVASSLDGKPTRLAGTLRDVTMSKQVEEA
                     LRLESDRFQSLVHNSFDIVLVVDARGLIKYVTPSTVQLLGYEADAIVGTNAFSYVHPD
                     DTEFLIREFNQDVLTKTNKGIPSAFRAWKADG"
     gene            1406..2998
                     /locus_tag="PK710_17270"
     CDS             1406..2998
                     /locus_tag="PK710_17270"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOT11532.1"
                     /translation="MNRKDKERVQVEVVQRVLDEVGTDAQRVEQAVFDTLYEERKRLE
                     TEPDGPSKSEQLAFYERMHTRSLRMDVQSQRKALKQITHRFVQEVTGHFDPRVYALST
                     RLVPVGLNLLLNALSPIKLVQSIPLGMSDLEEQFEVSGHVQRVKSLAQKATLVMVPTH
                     SSNLDSILMGYAIWRMGLPPHLYGAGLNLFSNKLIGFFMHNLGAYRVDRRKKAKIYKD
                     VLKAYAGCSMELGYHNLFFPGGTRSRSGRVEQGLKLGLLGMGLDAYVRNLIEKKVRPD
                     IFVVPCTINYELVLEAETLIADYLKEAGKARYIIEDDEFSKPKVILDFTKKLFSLQSK
                     IHVVLGEPLDVFGNRVDDDGVSRDFRDRPIDRTRYVIKNGIVQLDAQRDGEYTRELAH
                     AIVQSYRKHTMLKTTNVLCHVVFRWLQEKSQGMDLYRLLRTGGAEESIPIPEAYGRID
                     KVREALRRLADRERVRLDLPLREFETPTLVHYALAHLSSYHRRAAIERRGDRLFHEDR
                     NLIYFYQNRLDGHVAELQAELS"
     gene            2995..4017
                     /locus_tag="PK710_17275"
     CDS             2995..4017
                     /locus_tag="PK710_17275"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOT11533.1"
                     /translation="MTAMTTDLCIVGGGSFGSALAMIAAAQGHRVRMWVRREQQANEI
                     NQAHTNGAYLGEARLPDHLQATTDMQLAVQGATVVLMAIPSKSFREVARTVGEHLRGD
                     QLLVHATKGIEAATFARMSQILREETCALKIGVLSGPNLAAELMQGHPSGSVIASPYD
                     EVVHGVQALFSHSHLRVYGGRDVVGVEVGGAFKNIIAIAAGVADGLGFGDNARSLVIT
                     RGLSEMAWLGTHLGADVFTFGGLAGIGDLVATCNSKLSRNHRVGERLALGKDLDTILR
                     ELKFTAEGVPTTDAVHRRAAAAGLHLPICQAVYSVLFDGKNATGAFGELMRLPVGDEL
                     STLRYR"
     gene            4014..4946
                     /locus_tag="PK710_17280"
     CDS             4014..4946
                     /locus_tag="PK710_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11534.1"
                     /translation="MKSHPCCYPLSVVDDRIFSLDYDGPVFIWDIDKTYLATRFSQPK
                     YMARIPIEFAVDKQAIAGMPEVLRGIRRGTTAAFACAPLYFVSASPPQLRAVITRKML
                     LDGVEYDGITFKDWAKTIMQRRPGRLKEQVGFKICALLEGRRRRPMASEYLFGDDVEK
                     DAVAYWLYAKIIKGELSSAKMEEALAAQGVKADDRACIRAMRDALPPTLGSVRQIFIH
                     LEKKSSPVEFERFGPLLLTTRNAFQLALALADRSVVPIDTVRQAALALQAVAPFGNVD
                     FDELIVDARTRKLVSSIPEEVMASFASSSSMDIR"
     gene            complement(4984..5820)
                     /locus_tag="PK710_17285"
     CDS             complement(4984..5820)
                     /locus_tag="PK710_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11535.1"
                     /translation="MNRMQLGGLCLAVTTLCAGCSGNDERPPGVGGCTDVLCGKPGGG
                     TVNPRPDGGQPEAAIDGQQADVDAAPISTVDLSGSIVRLTEEQFSQEVPFLGWGYLRV
                     PTVSGDDQVSFGGEAGVGFSATGVVSGPGWFTVVPEPAFYDLVIPTHAYLIAPQQASS
                     NFTVPVVDRDLLTSIYASLSPPAFVRADASHVIVVFEKNGQRVAGVSITTHPTAEAIA
                     YDQGVGYSTEGTETGVNGTVLLVNVVGMSPLRWKDNTGTDGSLTLVHVIGQVSFVRID
                     LS"
BASE COUNT         1344 a         1620 c         1652 g         1313 t
ORIGIN      
        1 accatcggct ttccatgcgc gaaacgcaga aggaattccc ttgttcgtct tggtgagaac
       61 gtcttggttg aactcccgaa tgagaaactc ggtatcatcg ggatgaacat acgaaaaagc
      121 gttggtgcca acgatggcgt cggcttcata gccaagcaac tgaaccgtcg atggtgtcac
      181 atacttgatg agtccccggg catcgaccac caacacaatg tcgaacgaat tgtgtacgag
      241 cgattgaaaa cgatcgcttt cgagacgaag tgcctcctcg acttgcttgc tcatcgttac
      301 gtcccgcaac gtacccgcca accgcgtcgg tttgccgtcg agcgaagaag caacgatgcg
      361 tccggaagca tggacccata tccaacgacc cgcgcgtgtc atgacgcgga attcctcgga
      421 ccacgaggtt gccgcccctt tgacatgctc gcgcagcgct tggatcgcgg cttcgacgtc
      481 ttccggatga atcaacgcac gcacttgatt caatccccgc gcgagactcc cttcgggata
      541 cccaagcatc gattcataat acgcaatcga aaaacccgta tcgtcgggaa gggaccactc
      601 ccaaagcgcc gtcgaggaac cttcaaacgc caattcgagg cgttgttgag tgacttccag
      661 cgctttttcc acgtcgagac gatcatggct gtcctcgaag gacatgacga cgtagggctc
      721 atcgttcgcg tggaaaggtg cggcagacaa cagacattcg atggtgttgc ccgcgacggc
      781 atgcaggcga acaggatgat tggcaacgga gttttcgaag aaggaaagga cgtcgtcgat
      841 gctttgcccc tcggcctttc ccttgaacaa ctctatgtcc gttagggttc gtccgacgat
      901 ttgatcgcga gaaaaaccag tgactcggct catcgcctga ttgatttcga gaatcgtgtg
      961 gtctgtgcgg tgcaaaatca ccaaagggtg cgggcaagcg tcgaacgctt tttcaaggac
     1021 cggggcccag gggtgttcat caggacgtct catggattcc gcgggggatt cctgctcgac
     1081 tcgaaagcgc tcgctcatct gatctcctgg atgaaaccgt gacttcggtt atcccctggc
     1141 gacaattcga tttatttcta tatacaaata cttgcgcccg cttgggagga atggattccg
     1201 gtcgctaggg cgggcacagc accgagggat tcaatatctt gtatgaaaac cttgggacag
     1261 gatagcaaga ggcgtgaaaa aattcgttgt aatttcagta tattacagcc gattgccggg
     1321 aagctgtgcg gctcatgctc ttggttgacc aaggattact tgcgtgatgt gataggccaa
     1381 ctcttctcga atgggtggct atcaggtgaa ccggaaagac aaggaacggg ttcaggtcga
     1441 ggttgtccag cgtgtcctcg acgaggttgg tacagatgcg caacgtgtgg agcaggctgt
     1501 attcgacacg ctatacgaag agcgaaaacg tctcgaaacc gagccggacg gcccttccaa
     1561 aagcgagcag ttggcgtttt atgagcggat gcatacccgt tcgttgcgga tggatgtgca
     1621 gagccagcgc aaggcgctca aacagatcac gcatcgattc gtgcaagagg tgacgggtca
     1681 tttcgatccc cgggtgtacg cgctgtccac gcgtttggtc ccggtggggt tgaatcttct
     1741 gctcaatgcg ttgagtccca tcaagttggt gcagtccatc ccgctgggca tgtcggattt
     1801 ggaagagcaa tttgaagttt cgggccacgt ccaacgggtc aagtccctcg cacaaaaagc
     1861 aacgttggtc atggtcccca cccattcgag caatctggac tcgattctga tgggatatgc
     1921 catctggcgc atgggccttc ccccgcattt gtacggtgcg ggattgaatc tgttcagcaa
     1981 caagctgatc ggatttttca tgcataattt gggggcctac agagtcgatc gacggaaaaa
     2041 agccaaaatc tacaaggatg tgctgaaggc gtatgccggc tgcagcatgg aactggggta
     2101 tcacaatctg ttctttccgg gaggaacgcg cagtcggtcc ggtcgtgtag aacaaggctt
     2161 gaaactaggc ctgcttggca tgggcttgga cgcctacgtg cgcaacctca tcgaaaaaaa
     2221 agtccgcccc gacatcttcg tcgtgccatg caccatcaac tacgaactcg ttctcgaagc
     2281 cgaaacgctc atcgcggatt acctgaaaga ggccggaaaa gctcggtaca tcattgaaga
     2341 cgatgagttt tccaagccca aggtcatcct cgacttcacc aaaaaactct tctccctcca
     2401 atcgaaaatc cacgttgtgt taggcgagcc tctcgatgtt ttcggaaacc gtgtggacga
     2461 cgatggtgtg tcccgcgatt tccgagaccg accgatcgat cgaacgcgtt acgtcatcaa
     2521 aaacggaata gtccaactcg atgcgcaacg ggacggagaa tatacgcgcg aactcgccca
     2581 tgcgatcgtg cagtcctatc gcaaacacac gatgctgaag acaaccaacg tgctttgtca
     2641 cgtcgtgttt cgctggttac aagaaaaatc ccagggaatg gacctgtatc gcttgttgcg
     2701 aactggagga gctgaggaaa gcattcccat tcccgaagcc tatgggcgca tcgacaaggt
     2761 gagagaggcc cttcgacgcc tcgcggaccg tgaacgcgtt cggctcgacc tgccgttgcg
     2821 tgagttcgaa acgcccacgc tcgttcatta cgccttggcc cacttgagtt cctatcatcg
     2881 ccgcgcggcc atcgaacgac ggggggatcg gctctttcat gaggaccgaa acctcatcta
     2941 tttctatcaa aaccgtctcg atgggcatgt ggcggagcta caggcggagc tgtcatgaca
     3001 gccatgacta ccgatttatg catcgtcgga gggggaagct ttggttcggc gcttgccatg
     3061 atcgcagccg cgcaaggtca tcgcgtgcgg atgtgggtgc gtcgcgagca gcaggccaac
     3121 gagatcaatc aagcgcatac caacggagcg tacctggggg aggcgcggtt gccagaccat
     3181 ctgcaagcta ccacggatat gcaattggct gtgcagggag caaccgtggt gctcatggcg
     3241 ataccctcca aatcctttcg cgaggtggca aggaccgtgg gtgagcatct tcgaggagat
     3301 cagcttttgg tgcatgccac caaagggatc gaagccgcca cctttgcgcg tatgtctcag
     3361 attttacgag aagaaacctg cgcgctgaaa atcggagtgc tgtcgggacc caatctcgcc
     3421 gcggaactca tgcaaggtca cccctccggc tcggtgatcg catcgcccta cgatgaagtc
     3481 gtccacggcg tgcaagcttt gttctctcac agccatcttc gcgtctatgg tggtcgcgat
     3541 gtggtgggtg tcgaagtggg aggcgctttc aaaaacatca tcgccattgc cgcgggagtc
     3601 gccgatggcc tgggtttcgg agataacgct cgctctctcg tcatcacgcg aggactcagt
     3661 gaaatggcct ggttgggcac tcatctcgga gccgatgtat ttaccttcgg cggcctggcc
     3721 ggtatcggag acctggtggc cacgtgcaat tccaagctga gtcgcaacca ccgggtggga
     3781 gagcgtctgg ctctaggcaa ggacctcgac acgatcttgc gtgagctgaa attcaccgct
     3841 gaaggtgttc ccacgaccga tgcagtgcat cgacgagccg ctgccgccgg tcttcatttg
     3901 cccatctgtc aggctgtgta ttcagtgctc ttcgatggaa aaaatgcgac tggcgcgttc
     3961 ggtgaattga tgcgtttgcc cgtcggcgac gaactgtcga cgttgcgata ccgatgaagt
     4021 cccacccttg ttgctatccc ctgagcgtcg tcgacgaccg gattttttct ctggactatg
     4081 atggtcccgt tttcatttgg gatatcgaca aaacctatct ggccacacgt ttttcacagc
     4141 ccaagtacat ggccagaatc cccatcgagt ttgcggtgga caaacaagcc atcgctggaa
     4201 tgcccgaggt gctgcgaggt attcgtcgag gaaccaccgc cgcatttgct tgcgcgcctc
     4261 tttacttcgt ttcggcgagt cctccgcaat tgcgcgccgt catcacgcga aaaatgttgc
     4321 tcgatggcgt ggaatacgac ggaataacct tcaaagactg ggcaaaaacc atcatgcaac
     4381 gacgccccgg tcgcctgaag gaacaagtag gcttcaagat ctgtgcgttg ctcgaggggc
     4441 gacggcgacg gcccatggca agcgagtatc tctttggtga tgatgtggag aaagacgcgg
     4501 tcgcctattg gctgtatgcc aaaatcatca aaggcgagtt gtcatccgcc aagatggaag
     4561 aagccttggc tgctcaaggc gtaaaagctg acgatcgcgc atgcattcgc gccatgcgcg
     4621 atgcgttgcc cccaaccctt gggtccgttc ggcaaatctt catccatctc gagaaaaagt
     4681 cgtcacccgt ggaattcgaa cgctttggcc cgttgcttct gaccactcgc aacgctttcc
     4741 agctcgccct cgctcttgcc gatcggtcgg tggtccccat cgatacggtt cgtcaagcag
     4801 cgctcgctct gcaagccgtg gctccctttg gtaacgtgga tttcgatgaa ttgatcgtgg
     4861 atgcgcgaac acgaaaactc gtttcctcga ttccagagga agtgatggcc tcgttcgcgt
     4921 cttcgtcatc gatggatatt cgttgaacga ttccccttag acgcactgat agacagactt
     4981 tcttcaagac aagtcgatcc ggacgaaact cacttgtcca atcacgtgta cgagcgtaag
     5041 ggagccgtcg gttccagtat tgtctttcca ccgaagagga ctcattccca cgacgttgac
     5101 aagcaggacc gtgccgttga cgcccgtttc cgtgccttcc gtcgaatatc ctaccccttg
     5161 atcgtaggcg atggcttccg cggttggatg ggttgtgatg ctaacgcctg ccacgcgttg
     5221 accgttcttc tcgaaaacga cgatcacatg agaagcatcg gcgcgaacga aggcgggagg
     5281 agacaaagaa gcgtagatcg aggtcagcag atcgcgatcc acgacgggta cggtgaagtt
     5341 cgaagaagct tgttggggtg cgatcaggta agcatgcgtg gggatgacga ggtcataaaa
     5401 tgcgggttcg ggaacgaccg taaaccaacc aggtccgctg acgactcccg tagcggaaaa
     5461 accaactcct gcttctcccc caaaactcac ttgatcatca ccggataccg tgggcacgcg
     5521 aagataaccc catcccaaaa agggaacctc ctgggagaat tgctcttcgg ttaggcgaac
     5581 aatcgatccg gataggtcga ccgtcgagat gggagccgca tccacgtccg cctgttgccc
     5641 atcgatggca gcctctggct gcccaccatc aggtcgtgga ttgaccgtac cacctccggg
     5701 tttgccacag aggacatccg tacagccacc aacaccagga ggacgctcat cgttgcccga
     5761 acaccctgcg catagggttg tcactgccaa gcaaagaccg ccgagttgca ttcggttcat
     5821 cgtcttttac tgtacgctcc ggctgatgaa gtggccaacc accaggctca tgcttcggcg
     5881 atcgtttctg gaatcactgg caattttttt atgcgatgaa catgaaaca
//
LOCUS       DAOSOR010000366         5907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13639_length_5907_cov_4.437286, whole genome shotgun sequence.
ACCESSION   DAOSOR010000366 DAOSOR010000000
VERSION     DAOSOR010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13639_length_5907_cov_4.437286"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /gene="sdaAA"
                     /locus_tag="PK710_17290"
     CDS             complement(<1..1288)
                     /gene="sdaAA"
                     /locus_tag="PK710_17290"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOT11536.1"
                     /translation="MAFTSLDELLLTAQQQQRPIYELMIESEIRQKGISREEIIIKMT
                     EQFAVMEEAVREGTHASVVSRTGLTGGDGHRLYEYAKRGQSLVEPSSLLTAAYALAVS
                     EVNANMGRIVATPTAGSAGILPAVLVHALDSGRFSREQIVLSMFTASAIGLVIANRAS
                     ISGAAGGCQAEVGSATAMAAGTLVELAGGTPQQVGNAVGIALKNSLGLVCDPVAGLVE
                     IPCILRNGLHAIAAQAAADMALAGVVSLIPPDEVIHVMHEVGQHMPESLRETGIGGLA
                     GTPTGQKLKEQVFGNEKEQKRGGGGPAKYQSAYEIIGPIMIGPSSSHTAGAVRIGNIA
                     NQLLKEPPKRVTFSLMGSFATTYEGHGTDVALLAGVLGLSTRDKCISEAKQLAAEQGL
                     AYTFMKRVLGSYHPNTVLVELEGARRTVKLLASSLGG"
     gene            complement(1980..2642)
                     /gene="elbB"
                     /locus_tag="PK710_17295"
     CDS             complement(1980..2642)
                     /gene="elbB"
                     /locus_tag="PK710_17295"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HOT11537.1"
                     /translation="MGKRVAVVLSGCGYLDGAEIYESVSVLLALDLEGAQVQCFAPDK
                     AQMHVVHHGTQTPVQGETRNVLTEASRLARGQIKAIEEARMEDFDALVFPGGYGVAKN
                     LCDYAVKGSDCTVDPRVDALIKAAHGAKKPICAICIAPVLIAKSLGLAHHPTLTIGSD
                     AGTAADIEKMGGKHVNAPVTEIVVDEQNRVVTTPAYMCGQRIGDVWLGVKKAVEKTLS
                     MA"
     gene            2655..3158
                     /locus_tag="PK710_17300"
     CDS             2655..3158
                     /locus_tag="PK710_17300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11538.1"
                     /translation="MGRLTSSTRPKQDDSASETPAKPTIHQPLHQPSRHEGLGILNPG
                     GRGGFVVPVDVPVDVVVDVGGGDVVVPVDVPVEGVMIIHGGRRVVLVAVAVLVAVLVP
                     VAVLVAVAVLVAVAVAVEGGRAVDVVGGLDVVVEVGVVVTATGSVVCTGSIDGKGSEP
                     LSTKPSL"
     gene            3895..5676
                     /locus_tag="PK710_17305"
     CDS             3895..5676
                     /locus_tag="PK710_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11539.1"
                     /translation="MRCITMWIALIGAVGAAAMGCTFVEAADDDQGPGASGAAGTAGS
                     GGAAGSGGVAEGGTGGGVDEDPCHGVPMQGQCIDNMTIRACLHPDGNTSSGHVDIVET
                     KCLSGRVCKEGTNGAYCAVSGVCIPGDIECTADSRSVRVCEGSGDDTHWVVTPCDTAA
                     GEKCQPGTPTTPAACHYVPSSSGGTSGPTLHGRMKYEYRPVRPDGMGWGDLQVQDIRD
                     LYITVYDDGEFIGAALSGYDSVAEDFTTDSSFVAKLTREMRGPTSLWAWPIVFNYDTG
                     LPAMAVAKLKHQNPMVNAREANEYWAWGLDIQPGTTDIGTWTITEKQYSGALHIFQWI
                     DYGLLRAAAGMQGDQTSLAVFWDPVVGTPTCGACYCGPACGGAQIQYGSSSSESDYYD
                     NWIVLGGPPSDGSTQWARAVISHEFGHYVMQNYSKSPGEGGTHYVDQASKPGLAYSEA
                     WATAFGCTNIESPKYVDEQMGSFFWVDISKYTYSGSGGKLQMPDPNGPIDQYINENVG
                     AGMIWKLFVDSSVDPQGRGLGDDKVFGALTYPPLVDGTFNRGYHTVDLVDFFDAALCS
                     GYVSEADINAVTKTTGFPYNPATRPCL"
     gene            5703..>5907
                     /locus_tag="PK710_17310"
     CDS             5703..>5907
                     /locus_tag="PK710_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11540.1"
                     /translation="MTTMTTGLGIGLLCAFISMGACTPNGNSGGGTSAQPYQQPQNTP
                     QQMEPQRGISAPVVLNLRGPNPVP"
BASE COUNT         1288 a         1772 c         1603 g         1244 t
ORIGIN      
        1 ccccacccaa cgaactggca agcaatttca cggtccgccg ggctccttcg agttccacaa
       61 gcaccgtatt cggatgataa ctacccagta cgcgcttcat aaaagtataa gcgagccctt
      121 gttctgctgc taattgcttt gcctccgaaa tacacttgtc cctcgtcgac aaaccgagca
      181 cacccgcaag caatgcgacg tctgtgccgt gcccttcata agtggttgca aacgagccca
      241 tcaacgaaaa cgtgacgcgc ttcggtggtt ctttcaacaa ttggttagcg atattcccaa
      301 ttcgcacggc accggcggta tgggagctgg aaggaccgat catgatagga ccaatgattt
      361 cataggcgct ttggtatttt gccggcccac caccgcctcg cttctgttct ttctcgttac
      421 cgaatacttg ttctttcagt ttctgtcccg tcggcgtgcc tgccaggcca ccgatacctg
      481 tttcacgcaa tgactcaggc atgtgctgcc caacctcatg catcacatga atgacttcat
      541 caggcggaat caggctgacc acgccagcca atgccatgtc tgccgccgct tgcgcggcaa
      601 tcgcatgcaa accattgcga agaatgcacg gaatctcgac gagtcctgct acgggatcac
      661 agaccagtcc gagtgagttt ttcagcgcga ttccaacggc gttgccgact tgttgcggtg
      721 ttccgcccgc aagctcgacc aacgtgccag cagccatcgc cgtagctgaa ccgacttcgg
      781 cctgacaacc gcccgcggca ccagaaatcg acgcccggtt cgcaatgacc agaccgatcg
      841 ccgaagccgt gaacatcgac aagacaatct gctcgcgcga aaatcgccca ctatcaaggg
      901 catgaacaag tacggcaggc aaaatgccgg cagagcccgc cgtcggtgtc gccacgattc
      961 gccccatatt ggcgttcact tcggaaaccg caagggcata cgccgccgtc aataacgaag
     1021 acggctcgac caaggactgt ccacgcttgg catattcgta taaacgatgc ccatcgccac
     1081 ctgtcaagcc agtccgcgat acgacggaag cgtgggtacc ctcgcgcacg gcttcctcca
     1141 tgaccgcaaa ttgctccgtc attttgatga ttatctcttc acgggaaatc cctttttgcc
     1201 gtatttccga ttcaatcatc aactcataga tcggtcgctg ctgttgctgt gccgtgagca
     1261 gcaactcatc caaactcgta aaggccatcg acgtcactcc acaaaaaata acaatccctc
     1321 tttcacggct tacaagttca tacactgcaa caccatagga agggcgggaa cgaatacaac
     1381 aactccgagg ttcgttccag atcaggttct cgtcgtagga acccacgaaa cctcacgaag
     1441 cggagccatc cccccatttt tccccaagcc tttccgtcgt cctacgactg ccgcaaacaa
     1501 gtacctacca tcgtccggcc gcggtcacgg cccacgagaa acggtcacgg tcatcccata
     1561 ttattcaccg caaaacacca gtccaatgtt tagtagccca gggcaactgt tgggggtgct
     1621 ccaaaggggg ttcaaggacc gagccaaaat caagatcgca agaattctca gctcgtaccc
     1681 ctttccaatt cccgaaaaat ttcggggatc tgtcaggatt tcgcctccgg gacacgtcgc
     1741 tgtctcaagc tcccaatcgg aagcgaggcc aattcacaaa gaaaaaaata tgacctatgg
     1801 tgctgtatcg acagaattgt gtcggtttag ataattagct tataaaatcc aatagttacg
     1861 agtctaggga tcgaccaaag gatcaagatg gggtgataca gcccccgaac ggtgtgcgtg
     1921 ggattggcac catcgcggcg gatggcaggc tctgccctta cccaaggttt tgggatggct
     1981 caagccatgg aaagcgtttt ttccaccgcc tttttcaccc ccaaccacac atctcctatc
     2041 cgctgtccac acatataggc gggcgtggtc accacccgat tctgctcatc gaccacgatt
     2101 tcggtgaccg gcgcgttcac atgttttccc cccatttttt cgatatccgc cgctgtcccc
     2161 gcatcactgc caatcgtcag cgtcggatga tgagccagtc ccagagactt cgcaatcaac
     2221 acgggcgcga tgcaaatcgc gcatatcggt tttttcgccc catgcgcggc tttgatcaag
     2281 gcatctaccc gcgggtcgac cgtacaatcc gaacctttca ccgcatagtc gcaaaggttt
     2341 ttcgcaacac cataaccgcc tggaaaaacg agtgcgtcga agtcttccat cctcgcttcc
     2401 tcgatcgctt tgatctgtcc gcgcgcaagt ctcgaggctt ccgtcaatac attgcgcgtc
     2461 tccccctgca cgggcgtttg ggtgccatga tgcacgacat gcatctgcgc cttgtccggc
     2521 gcaaagcatt gcacctgcgc cccttccaga tcgagcgcca atagcaccga caccgattcg
     2581 taaatctctg caccatccaa atatccacat ccgctcaaca ccacagcgac acgtttgccc
     2641 atcacgacac ctccatgggt cggttgacct catccacacg ccccaaacaa gacgattcgg
     2701 cttctgagac gcccgcgaag cctaccatcc accagccctt gcaccaaccc tctcgtcacg
     2761 aaggcttggg aattctgaac cccggcggtc gcggcggctt cgtcgttcct gtcgatgttc
     2821 ccgtcgatgt ggttgtcgat gtgggagggg gagacgtcgt cgttcccgtg gatgtccccg
     2881 tcgaaggcgt aatgataatc catggagggc gacgtgtcgt gctggtggca gtcgcggtgc
     2941 tggtagcggt gctcgtgccc gtggcggtgc tcgtggccgt agccgtgctg gttgcggtgg
     3001 cagtcgctgt cgaagggggg cgcgcggtgg atgtcgtggg aggtctcgac gttgtggtgg
     3061 aggtgggggt cgtcgtgacg gcaacgggct cggtagtctg cacgggttcg atcgatggca
     3121 aaggctcgga gccgttgtcg acgaaacctt cgttatgacc ttctgcttcg atcgtggcgg
     3181 ggggagcgaa cgtcacgatc ggagcgtcat tgtcgtcgct gctcgtggac cacataccgc
     3241 gagcgaaaag cacggcagcc gcggcgcccc cgcccatgag cagcacgaca aagccgacgg
     3301 caaaaaagag aattgctcct ccaccgccgc tttttcgtct tggaggcgcg tacggtgcgt
     3361 agccaatggg tccgggttgt atggctgaat acattggcgc gggcgagtag tcaggtgccg
     3421 tggaaccagg catgatgccc ggagcgaccg aggcatccac catcgtgaac gacggagtgg
     3481 cagcgcctcc ttcgggaatc gcggcctgtg gacccaattc gctgaagtcc atgggtggac
     3541 ctccgggaat ggtgccgcct ctgcgcccca tacccccgta cccgtcgtcc ggcgggtcga
     3601 agtttccggg tggttgattc atctggcctg tctcctatgc gcacacactg cagcgtcatt
     3661 ttgatattta ccggaaatcg caccgttctg ccagaccggt cgtagggcct ttcgtttctt
     3721 ccctattccc ccgttcccaa cgcaaccgta gcagaagcat tcgcggtaaa cggatcattc
     3781 gctaatcaac cggcctcacc tggccaaccc tgtcattttg aggaatcatt tcggaacgac
     3841 accgtatgat acaaagtgct ttcgtttttc gttaccagtc gtgggagagg gtctatgcgt
     3901 tgcatcacta tgtggatcgc attgattggc gccgtgggcg cagcggccat gggctgtacc
     3961 ttcgtggagg cggccgacga tgaccaaggc cccggcgctt ccggagccgc cggcacagca
     4021 ggctcgggag gtgcggccgg ctctggtggg gtcgccgaag gcggcacggg cggcggtgtc
     4081 gacgaggacc catgccatgg ggtgccgatg caaggacaat gcatcgacaa tatgaccatt
     4141 cgcgcctgtt tgcaccccga tggcaatacc tcgagcggcc acgtggatat cgtcgaaacg
     4201 aaatgccttt ccggacgcgt ttgcaaagag ggtacgaacg gcgcgtattg tgccgtttct
     4261 ggcgtatgca ttccgggcga tatcgaatgc accgcggatt cccgttccgt gcgcgtttgt
     4321 gaaggcagcg gcgacgacac gcattgggta gtcacgcctt gtgatacggc ggccggagag
     4381 aagtgccagc caggcacgcc aacgactccc gcagcttgcc attacgtccc gtcttccagc
     4441 ggtgggacga gcggccccac gctgcacggc agaatgaaat atgagtatcg accggtacgt
     4501 ccggacggca tggggtgggg tgatctgcaa gtgcaggata ttcgcgactt gtatatcacc
     4561 gtgtatgacg atggggagtt cattggcgcc gcactatccg gttacgactc ggtagcggaa
     4621 gactttacga cagacagttc gtttgtcgcc aaactcacgc gagaaatgcg agggcccaca
     4681 agtctatggg catggccgat cgtcttcaac tacgacaccg gtctgccagc catggccgtg
     4741 gcgaaactca agcaccaaaa cccgatggtg aacgcaagag aagccaacga atactgggcc
     4801 tggggtctgg atatccaacc gggcaccacc gacatcggaa catggaccat aacggaaaaa
     4861 cagtattcgg gagccctgca catcttccaa tggatcgatt acgggttgct tcgcgcggcg
     4921 gcgggaatgc aaggtgatca aacctccctc gccgtattct gggatcccgt cgtgggaacg
     4981 cccacgtgtg gcgcctgcta ctgcggccct gcctgtggcg gtgcgcaaat tcaatacgga
     5041 agcagttcat ccgaatcgga ttattacgac aactggatcg tgttgggagg ccctccgagc
     5101 gatggctcca cccaatgggc tcgcgcggtc atttcccatg aatttggcca ttatgtgatg
     5161 caaaactact ccaaatcgcc cggggaaggc ggcacccact acgtcgatca ggcctccaaa
     5221 ccgggccttg cctacagcga agcctgggcc accgctttcg gttgtacgaa catcgagagc
     5281 cccaagtacg tcgacgaaca aatgggttct ttcttctggg tcgatatctc gaagtacacg
     5341 tattcaggtt ctggtggcaa gctgcaaatg cccgatccga acggtcccat cgaccaatac
     5401 atcaacgaga atgtcggcgc gggcatgatt tggaaacttt tcgtggactc atcggtggat
     5461 ccacaagggc gcggacttgg cgatgacaaa gtattcggag cactaaccta tccgccattg
     5521 gtcgatggga cgttcaaccg tgggtaccat acggttgact tggttgactt cttcgatgcc
     5581 gcgctttgct ctggctacgt aagcgaagcg gatatcaatg ccgtgacgaa gaccactgga
     5641 ttcccttaca atcctgccac aaggccgtgt ttgtgatggg cggagccatg aggatgaaca
     5701 acatgacgac aatgactacc gggttgggga tcggattgct ctgcgccttc atttcaatgg
     5761 gggcttgcac acccaatggc aacagcggcg gaggaacgag cgcgcaaccc taccaacaac
     5821 cacaaaatac accgcaacag atggaacccc aacggggtat ctccgccccc gtggtcctca
     5881 acctgcgagg ccccaacccc gtaccct
//
LOCUS       DAOSOR010000367         5896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13670_length_5896_cov_4.756720, whole genome shotgun sequence.
ACCESSION   DAOSOR010000367 DAOSOR010000000
VERSION     DAOSOR010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13670_length_5896_cov_4.756720"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PK710_17315"
     CDS             <1..523
                     /locus_tag="PK710_17315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11541.1"
                     /translation="DLRYGAVLYRDLGDEYLTKTHPFSHDIVAFDRALQQVTAGGGGD
                     MPESLNQALAQAVAMPWREGAAKVAFLIGDAPPHMDYQGDVPYGDSAVRAIGKGLRIH
                     SVAASGLDAVGTLVFRQTAQLTRGKFIFIEYGSKEESARRHGVTGPVMSNNLDDIVYE
                     QIRDELEHWGKAR"
     gene            616..1446
                     /locus_tag="PK710_17320"
     CDS             616..1446
                     /locus_tag="PK710_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11542.1"
                     /translation="MSLSRAFSEPPNPSMGTRFRMRVSGWRFVLAIGLAGALGAAVYT
                     TQQLSLSSDPIEPCRSELAWEKMASNRSDLPVEMIEDDIVDPAAAPSSLATEKGKACV
                     MQPPLSEVELAEVLQEGYQIYFGHAASANRLACAWAHCAFEHARGAKLFGNNLGHITT
                     AGAWQGPTCEKSFTHRVSQNPDRWATSSLTFRVHPTLSAGAVDYWKLMDAQYPYVMMA
                     CDRGDTLAAARELRRRNYFTGPEDRYVQSLVRLYLEGMGGVLTKMEKPPWPIGPKTSQ
                     "
     gene            complement(1450..1734)
                     /locus_tag="PK710_17325"
     CDS             complement(1450..1734)
                     /locus_tag="PK710_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11543.1"
                     /translation="MSGQKSLCGPHVELVKTEGVRITRCPCGTLHLTLSKNGATIQLS
                     PEYFAEVVQALGLARTVLAGLQPSAEHPTTVLDPGRFVTLSPFDPKKPSN"
     gene            1913..2818
                     /locus_tag="PK710_17330"
     CDS             1913..2818
                     /locus_tag="PK710_17330"
                     /inference="COORDINATES: protein motif:HMM:NF039293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11544.1"
                     /translation="MATNPWHPPFRPGTLLEEIQRAEKHGQSSGALEPIPTSFERLEQ
                     NGIPFIVRVVDNLTRKPKAYCAVQSKASSDPFLPYEPDLYVADVSDSHVAVLNKYHVM
                     DRHLLVVTRQAEDQENLLTVADFFALWRCLREVDGLGFYNGGEIAGASQRHKHLQLVP
                     FPLSEGVDGVPIAPVVLDALEQGKRVVDRFAFRHAVAPCEEGWQRDPHAAACSCHSRY
                     LELFREVGIEITSRTIRQPMSYNFLMTRRWMMLVPRSSEKYEGISINGIGYAGGLLVR
                     NATELALVRAAGPMEILKKVGISIS"
     gene            complement(2831..3580)
                     /locus_tag="PK710_17335"
     CDS             complement(2831..3580)
                     /locus_tag="PK710_17335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11545.1"
                     /translation="MKHICIGMLVGLFLIHCGSDDEGGAKPAGGTGGNGGASGQAGQG
                     GEAGSGGSVETGGSSTGGSSVGGSAGTAGGGTVCDKRCEKTAELGCPNDAACVVLCQA
                     AIDYAPWCSDIMTSFVSCSEARPISDWSCDASGHSALSASACQNEAQAGVSCLAMGPP
                     GGMPDLTAECQATCQAMSSLPCAPKDCVSTCVDKIAHGPCAGAVGLVTVCGAKLTAAD
                     YQCVQNAPVPQDGKCSAEINLLAKCLQAHGG"
     gene            complement(3609..5864)
                     /locus_tag="PK710_17340"
     CDS             complement(3609..5864)
                     /locus_tag="PK710_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOT11546.1"
                     /translation="MRPALSALLLIAAVSLSACSTGRARFPSTASNLELSRVVLYRNG
                     VGYFERQGEVDGDVLRLRVRKDQVNDLLKSLTVVERTGGRAVSVSMPLDPQTWANAAL
                     ATLAPGRGSLAEVLDTLRGTQVSLTTASSTVSGRILMVEQVDMMPPTPSLRRGAPETE
                     PVTDHKVTLLDGDEMRVVLLSKVKGITLRDGDLAMQFHRSLDATAGEGMFQQVEVAIR
                     LVGRSAHDLLVSYVAPAPMWKPTYRVVLPEQGKGKALLQGWAVVDNTSGEDWRDVRLA
                     LTSGAPIAFRYDLHTPRDVDRTDLTETGVSKRARVAVGETSYERQATPSQVPSEMDAE
                     EGWDGDEEVAMGAVADARTIGGGKGGGSAPAGGMLARPKAAPPPSPAMRKADAPLAEP
                     APDTSVDFESLYRSTQASARAVAVSGLTRFDLSDEVTIPDGSSTMVAIVNQSVEAEET
                     FLFRPGGAGSGYEANPYRVVRFRNTTPFVLEPGPIAIYAGGSFVGEGLSEAVGTGTSA
                     TIPFAVESGIMVSSSTERSGEEMRLLRIVRGVLEVESFARTSTTWTVRSQTKNDGFTV
                     LIRHPKSGWNYELVDRPQGTEDLQGAYLIPIAVPQGKREAERKVVEQTPSKMQLSIWD
                     TRSVQLLDTLMLSGSISAADRTRLQPIVTLRQDIGRIDTQIDGLRRQRDDLDQRASET
                     RRSLEAIKKDAAAGALRAKLNKRLDEFTSEGDRIGREIVELQSKRLEKKIELEDLLQD
                     FDFRPSPSAGR"
BASE COUNT         1235 a         1727 c         1704 g         1230 t
ORIGIN      
        1 cgatcttcgc tatggcgcgg ttttgtatcg ggatttgggc gatgagtatc tgacgaaaac
       61 gcacccattt tctcatgata tcgtggcttt cgaccgcgcg ttgcagcagg tgacggctgg
      121 tggaggtggg gatatgcccg aatcgttgaa ccaggctctt gcgcaggccg tggcgatgcc
      181 gtggcgtgaa ggtgcagcaa aagtggcgtt tttgatcggc gatgcgcctc cgcacatgga
      241 ttatcagggc gatgttccgt atggggatag cgcggtgcga gccataggca aaggtcttcg
      301 catccacagc gtggccgcaa gtggtctgga cgccgtaggc acattggtgt ttcggcaaac
      361 ggcacagctt acccgcggaa aattcatttt catcgagtat ggcagcaaag aagagagcgc
      421 ccgtcgtcat ggggtcacgg gacctgtgat gagcaataac ctcgacgata tcgtgtacga
      481 acagatccga gatgagctgg agcactgggg gaaagccagg taatggcgcg cttgcgtccg
      541 caaccgtcat gaattgggcc atccgccgcc ggtcctggta gcgtttcgat ccagtcgtta
      601 cgaactgccg tactcatgag cctttcccgt gctttttccg agccacccaa cccatccatg
      661 ggaacacgtt ttcggatgcg cgtgtctggc tggcgattcg tgttggcgat cggcctggcg
      721 ggtgcgttag gagccgctgt ttacaccacc caacaactct cgctctcctc cgatcccatc
      781 gaaccatgcc gttcggaact tgcttgggaa aaaatggcgt cgaatcgttc cgatcttccc
      841 gtggaaatga tcgaagacga catcgttgat cccgctgccg cgccttcgag tctcgccacg
      901 gaaaaaggga aagcttgcgt gatgcaaccc ccgctgtccg aggtcgaact cgcggaagtg
      961 ctccaggagg gatatcagat ttactttggt catgccgcaa gtgccaatcg actcgcctgt
     1021 gcctgggctc attgtgcttt cgaacatgct cgcggagcca aactatttgg aaataacctt
     1081 gggcacatca cgacggccgg ggcatggcaa gggccaactt gcgaaaagtc tttcacgcac
     1141 cgcgtgagcc aaaaccccga tcgatgggca acgagcagcc tgacgtttcg cgtacatcca
     1201 accttatccg ccggtgccgt ggattattgg aagctgatgg atgcgcaata cccgtacgtg
     1261 atgatggctt gcgatcgagg agacactctc gcggccgcaa gggaattgcg tcgaagaaac
     1321 tactttacgg gccccgaaga ccgttacgtg caatccctgg tgcgcctgta tctggaagga
     1381 atggggggcg tcctgaccaa aatggaaaag ccgccctggc cgatcggtcc aaaaacttcg
     1441 caataagcgt cagttcgacg gttttttggg atcgaagggg gacaacgtga cgaaacgtcc
     1501 ggggtcgaga accgtcgtgg gatgctccgc ggacggttga agaccggcga gcacggtgcg
     1561 agccagtccc agcgcctgca cgacttccgc aaaatactcg ggcgaaagct gaatcgtggc
     1621 gccgtttttc gacagcgtaa ggtgcagggt tccacaggga cagcgggtga ttcggacccc
     1681 ttctgttttc accaattcca cgtgaggacc gcaaagagat ttctgaccgg acatgggcaa
     1741 agcagatagc gccaattggc accgatgcca agcccacctt gtcgtatcat tgacctaacc
     1801 ataggaatct catacagaat tgagcgtcgc ctccggccga tttgacctgt tcccacaatc
     1861 tacctggtcc acgatcggtg gctctccttc accacgacaa tccggaaatg acatggcaac
     1921 gaatccctgg caccccccgt ttcggccggg aaccctcttg gaagaaatcc aacgagccga
     1981 aaagcacgga caaagctctg gcgccctcga accgatccca acctctttcg aacggctgga
     2041 acaaaacggc atccccttca tcgtccgggt cgtcgacaac ctcactcgca aaccaaaggc
     2101 gtattgcgcg gtgcagagca aggcttcttc cgatcccttt ctcccctacg agcccgacct
     2161 gtacgtggcc gacgtctcgg attcacacgt cgccgtactc aacaaatacc atgtgatgga
     2221 tcgtcatctt ttggtcgtga cccggcaggc cgaagaccaa gaaaacttgc tgaccgtcgc
     2281 ggattttttt gcgctttggc gctgcctacg ggaagtcgac ggcctcgggt tctacaacgg
     2341 cggagagata gcaggagcga gccaacgtca taaacacctg caactcgtcc cctttcctct
     2401 gtccgagggt gtcgacgggg tgcccatcgc cccggtggtt ctcgatgcgc tggaacaagg
     2461 gaaaagggtg gtcgacaggt ttgcctttcg tcatgcggtc gcaccgtgcg aggagggttg
     2521 gcaacgagac cctcacgctg ccgcatgctc gtgccatagc cgatatctgg agctattcag
     2581 ggaagttggc atcgagataa cctctcgaac gatacgtcaa cctatgagct acaactttct
     2641 gatgacgcgg cggtggatga tgttggtgcc gcgttcgagc gagaagtacg aggggatttc
     2701 gatcaatggg attggttatg cgggaggatt gctggtgcgc aacgcgacgg aactggcctt
     2761 ggttcgcgcg gccggcccga tggaaatact aaaaaaagta ggaatttcaa ttagttagct
     2821 agttgtcgct tcatccaccg tgggcttgga ggcatttggc taagagattg atctccgcgc
     2881 tgcactttcc atcttgtggc acgggtgcgt tctgcacgca ttgatagtcg gcagcggtga
     2941 gtttggcgcc acagacggtg accaagccta cggcaccagc gcaaggaccg tgggcgattt
     3001 tgtcaacgca tgtggatacg cagtcttttg gagcgcaggg caagctggac atggcttgac
     3061 aggtggcctg acactccgcc gtgaggtccg gcatgccacc gggcggaccc atggccaagc
     3121 aagagacacc cgcctgtgct tcattctggc atgcggaagc cgaaagagcg ctatgtcctg
     3181 acgcatcaca actccaatcg gaaatcggac gtgcttcgga gcaagagacg aaggaagtca
     3241 tgatgtcact gcaccaaggc gcgtaatcga tagcggcttg gcagaggaca acacaagccg
     3301 cgtcgttggg gcagccgagt tcggccgttt tttcgcaacg tttgtcacag accgtgcctc
     3361 cgccagccgt tcccgcggaa ccaccgacag acgatccgcc cgtggatgag ccaccggttt
     3421 cgacagatcc tccggagcca gcctcgccac cttgaccagc ttgcccagaa gcgccgccgt
     3481 ttccacccgt tccaccggca ggtttggctc ctccctcgtc atcgctgccg caatggatca
     3541 aaaacaatcc gaccagcatt ccgatgcaaa tatgtttcat gacgtgtttt ccattcgagg
     3601 ggtgagtctc atcggcccgc ggacggagac ggacggaagt cgaagtcttg cagcaagtct
     3661 tccagctcga tttttttctc cagccgcttg ctctgcaact ccacgatttc acgaccgatt
     3721 cggtctcctt ccgaagtgaa ttcgtccaag cgtttgttaa gcttggcacg caaagcgccg
     3781 gccgccgcat cttttttgat cgcttcgaga ctccggcgtg tttcgctcgc gcgctgatcg
     3841 aggtcgtctc gttgtcggcg aagaccatcg atttgggtat cgattcgacc aatgtcctga
     3901 cgaagcgtca caatgggctg tagtcgtgtt cggtcggccg cggaaatgga gccagaaagc
     3961 atcaacgtat cgaggagttg cacacttcgg gtatcccaga tgctgagttg cattttggaa
     4021 ggggtttgtt cgacgacttt ccgctcggct tctcgctttc cttgaggaac ggcaataggg
     4081 atgaggtatg cgccttggag gtcttcggtc ccttgcggac ggtccacgag ttcgtagttc
     4141 caaccggatt tgggatgacg aatgagcacg gtgaagccat cgtttttggt ttggcttcgc
     4201 acggtccatg tggtggaggt acgagcgaag ctttcgacct cgagcacgcc gcgaacgatt
     4261 cgcaacaggc gcatttcttc cccggaccgt tcggtcgagg aagacaccat gatcccggat
     4321 tccacggcaa atgggatggt ggcgctggtg ccggttccga cggcttcact gagaccttcg
     4381 cctacgaaag agcctcccgc atagatggcg atggggcctg gctcgagaac gaagggtgtg
     4441 gtgttgcgga accgaacgac acgatagggg ttggcttcgt agccggaacc cgcgcctccg
     4501 ggtctgaaaa ggaaggtttc ctcggcctcg acggattgat tcacgatcgc caccatggtg
     4561 gaggaaccat cggggatggt gacttcatcg ctaaggtcga agcgggtcaa gccgcttacg
     4621 gcgacggcgc gtgcgcttgc ctgggtgctt cgatagaggg attcgaagtc cacggaggta
     4681 tcaggcgcgg gttccgcgag gggcgcatcg gcctttctca tggccgggga aggtggcggc
     4741 gccgctttcg gcctggcaag cattccccca gccggcgcag aacctccgcc tttgcctcct
     4801 cctatggtcc gcgcgtccgc gacggcaccc atcgcaacct cttcgtcgcc atcccaccct
     4861 tcttccgcgt ccatctcact gggcacctgg gaaggcgtgg cctgtcgctc ataactggtt
     4921 tcgccgacag caactctcgc gcgcttgctc acccccgtct ccgtcaaatc cgttcgatct
     4981 acgtcgcgcg gcgtatgcaa gtcataacga aaagcaatgg gagccccaga agtaagcgcc
     5041 agacgaacat cgcgccaatc ctcgccgctg gtgttgtcca ccaccgccca gccttgcagc
     5101 aacgcctttc cctttccttg ttccggcaat accacccgat aggtaggttt ccacatcggt
     5161 gccggcgcca cgtagctcac gagaagatcg tgcgcggacc gacccacgag ccgaatcgcc
     5221 acctccacct gttggaacat tccctccccg gccgtcgcat cgaggctgcg gtggaattgc
     5281 atggccaaat ccccatcgcg caacgtgatt cctttgacct tcgaaagaag aaccacgcgc
     5341 atctcatcgc catcgagcaa cgtaaccttg tggtccgtca cgggctccgt ttccggagcc
     5401 ccacgacgaa gcgaaggcgt tggcggcatc atgtcgacct gttccaccat caaaatccgt
     5461 ccggaaaccg tggaagacgc cgtggtcaac gacacctgcg tcccgcgcaa cgtatccaac
     5521 acttctgcca aactgcctcg tcccggagcc aacgtggcaa gcgcggcatt ggcccaggtc
     5581 tgggggtcga gaggcatgga gacactgacc gcgcggcctc ccgtgcgctc caccaccgtc
     5641 aagcttttca acaggtcatt gacctggtct tttcgaacac gcagccgcag cacatcccca
     5701 tccacttccc cctgtcgctc aaagtatcca acgccattgc gataaagcac aacgcgactc
     5761 agttccagat tcgacgctgt agaagggaaa cgcgcgcgcc ccgtggagca cgcggacaaa
     5821 gaaacggctg cgatcaataa aagtgctgat agagcgggac gcatggatag tacctctcgg
     5881 aaatagcggt cgctgt
//
LOCUS       DAOSOR010000368         5879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13721_length_5879_cov_4.072802, whole genome shotgun sequence.
ACCESSION   DAOSOR010000368 DAOSOR010000000
VERSION     DAOSOR010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13721_length_5879_cov_4.072802"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PK710_17345"
     CDS             complement(<1..552)
                     /locus_tag="PK710_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11547.1"
                     /translation="MRFFIATLALTGLALQGCQLIGGIEERTLVLQDAGADNDWEADV
                     QPEAEADVEPDVIDTTCELPTVGNASMRIGNLIPSLDRVEFCIKSANQTWQDVKPILA
                     GSGGGCPRGLAYRSLSAAFDVQAGVYDVIAVHDPATGSRPACTVTPIAEASQIVISEG
                     ETMSLLVFGDSRSNASLRALKETK"
     gene            complement(555..1583)
                     /locus_tag="PK710_17350"
     CDS             complement(555..1583)
                     /locus_tag="PK710_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11548.1"
                     /translation="MRIWLRPRSVAIAVAWGLTMSASPFALAQPTPEGKAIAETLFRD
                     GRALMDEGRYPEACRKLEESQRLDPALGTLLNMAVCHEKEGKIATAWAEFNEAMVLAR
                     REGRQDRFDLAQQHMTSLEPDLPKLVLNVPPQSRIPGLEILRNGTPIGVGAWGTPLPI
                     DPGEVIIEARAPKYKPWTTTLQIEIREQKSEDIPLLESMPEDELTTGASAGTGSSRRS
                     PPPPPEDFWTANRIAGVTLAGVGIAAMGIGSVFGVTALNNQSDSEAYCSGKTCSDQRG
                     IDLNKDAMRNANIANITIGAGALVGAVGALLFFSGGSDAPTEMQSGVDISFTMMPSQA
                     SAGVRGTW"
     gene            complement(1583..3037)
                     /locus_tag="PK710_17355"
     CDS             complement(1583..3037)
                     /locus_tag="PK710_17355"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT11549.1"
                     /translation="MLASSGEHGAVSVDIGDVLVGKYKVESILGQGGMGVVVAAMHLH
                     LQERVALKFLRPGAGRSEEFNARFMREAQVSAKLRNEHIVKVSDVGMLESGAPYMVME
                     YISGVDLRSMLKKEGPLAIPRAIDYIIQACEGLAEAHSFSVVHRDLKPSNLFVTTRRD
                     GTELIKVLDFGISKANLVSSSDLEDLTAAGALLGSPKYMSPEQLTNTAQVDERSDVWS
                     LGVVLYEMLAGTPPFVADTHAATCMRVLGTELPPSLCARRPDVPPELEAAIFRALERD
                     LNRRTPNVAEFAEDVIDAVGALVPHQVLVSIERIRRTIESHSLASSGATPRPRERRNI
                     LAESMTSSSFSPASGSGAVVTANKQPQRKGLLVGIAVLLSALIVALAALISQRSDNTD
                     PVMAGATVSVSATPSAPVAVVESPATSSTSIPAEPPRTAESIPSPGTTPEPTPTKVIP
                     GPTRWSPPPVRSTAPAPTGTPTSTKKVDPLEERQ"
     gene            complement(3209..4561)
                     /locus_tag="PK710_17360"
     CDS             complement(3209..4561)
                     /locus_tag="PK710_17360"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOT11550.1"
                     /translation="MVRTTKEAPVALEHGGMMRRVIHQILIAGLFGLALASCSSDEND
                     EQSPIQDTYIIDTFNIGLAGSFVPFEQQRLQPVMEAVASLNSDVVCVQEVWRKRDKEK
                     LAEIAKANFPYAVWFEHDFDTEVDDPADAHGMVPPAPTEPPCGVQGTKEKFEAVLQCL
                     LDNCSTLPGQREGKVTTATCAEEKCFVEAASLLAGAEQDKRCYGCAVTSLPEDTLGRI
                     EEKCTTNPKAALAFDGQSGVMILSRHPLINAEDWVLPATWNRRVILRATAKLPAGEVD
                     VYCNHLAPVFFDVAFPYTGDYGDGKTGPDAWAAEQKLQAEKLHAWLLKYSADRQAIVL
                     GDFNASLEFRENGRMVTRAEGPETVTLLQQHYTEAIPPDYPPACTFCSSNVLTGLTKE
                     WESAWVDHIFLHGISDQSILSAQRTLLDNTIRIENEGEELWVPVSDHYGMRAAIRLSP
                     "
     gene            4797..5291
                     /locus_tag="PK710_17365"
     CDS             4797..5291
                     /locus_tag="PK710_17365"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT11551.1"
                     /translation="MHPPSQPIADPPSAGKPPFAEPVGLHLRRARLQCGLTLQDISRT
                     TRISVSQLDYLERDRFEDLPGNLYTRGFIRSFAKAVGLDGELAVAHYTSNRRCRSISL
                     FPAQNHVEKKRRGLGIFVACSAFLLLIVVATLAVSHAKPKNAGRLLSFSALTHPGERP
                     PSLA"
     gene            5415..>5879
                     /locus_tag="PK710_17370"
     CDS             5415..>5879
                     /locus_tag="PK710_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOT11552.1"
                     /translation="MSERSELQREIDKRRTFAIISHPDAGKTTLTEKLLLYGGVVHLA
                     GAVKAKRGRANAVSDWMEMERERGISITTSVLQFEYKGLQLNLLDTPGHADFSEDTYR
                     TLHAVDGAVMLLDAAKGVESQTRKLFRVCRQRSIPIFTFVNKMDRPGRDPFEL"
BASE COUNT         1250 a         1618 c         1675 g         1336 t
ORIGIN      
        1 cttcgtttcc ttcaaagcac gaagggaagc gtttgaccgt gaatctccaa agacaagcaa
       61 gctcatggtt tctccttcgc tgatgacaat ctgcgaggcc tcagcgatag gagtgacggt
      121 acaagcaggg cgtgaaccgg tcgccgggtc atgtacggcg atgacatcgt agactcccgc
      181 ttgcacatcg aatgccgcgg ataacgagcg gtacgcaagg ccacggggac atccaccgcc
      241 gcttccggcc aagatgggtt tgacatcttg ccaggtttgg tttgcggatt tgatacagaa
      301 ctccacgcgg tccagcgaag ggatcaggtt tccgatccgc atcgatgcat tgccaaccgt
      361 cggcagttca caggtggtat cgatgacatc gggctcgacg tcggcctcgg cttcgggttg
      421 gacgtccgct tcccagtcgt tatcggcacc ggcatcttgg aggacgaggg tacgttcctc
      481 gattccgccg atgagttggc atccttggag agccaaaccc gtaagggcaa gggtcgcgat
      541 gaaaaaacgc atggtcacca tgttcccctc actcccgcgc tagcctgcga tggcatcatg
      601 gtgaaggaga tatccacccc ggattgcatt tccgtgggtg catccgatcc tcctgaaaaa
      661 aacagaaggg ctcccacagc gcccaccaac gcgcctgcac cgatggtgat gtttgcgata
      721 tttgcgttgc gcatggcgtc tttgttcagg tcgattcctc gttgatcaga gcatgttttc
      781 cccgagcaat aggcttcgct atccgactga ttgttcaagg cggtgacacc gaagacactg
      841 ccgataccca tggcagcgat accgaccccc gctagcgtca cccccgcgat tcgattcgcg
      901 gtccagaagt cttctggagg cggcgggggc gaacgacgag aagagccagt ccctgcggag
      961 gcaccggtcg tcaattcatc ttccggcatg ctttccagca gagggatatc ttctgatttc
     1021 tgttctcgga tttcgatttg gagtgtggtt gtccatggtt tgtactttgg cgcgcgggct
     1081 tcgatgatga cttcaccagg gtcgatgggc agcggtgttc cccacgcacc gacgccgatg
     1141 ggcgttccgt tgcggaggat ctcgaggccc ggaatacgcg attgtggtgg gacattgagg
     1201 acgagtttcg gcaaatcggg ttcgagggat gtcatatgct gctgcgccaa atcgaaccgg
     1261 tcttgtcggc cttcacgacg agccagcacc atggcttcgt tgaattcggc ccaggcggta
     1321 gcgattttgc cttccttttc gtgacatacc gccatgttga gcaaggttcc gagggcgggg
     1381 tcgaggcgct ggctttcttc gagttttcgg caggcttctg ggtagcgccc ttcatccatg
     1441 agcgcacggc cgtcacgaaa gagagtctcg gcgatggctt tgccttccgg tgtgggttgt
     1501 gcgagcgcga acgggcttgc actcatggtc aagccccaag caaccgcaat ggcgacagat
     1561 ctcggacgca accaaattcg catcactgac gttcctccag cggatcaacc tttttcgtgc
     1621 ttgtaggcgt tccagtgggt gccggggccg ttgatcgaac cggtgggggc gaccatcggg
     1681 taggccctgg aatcactttg gtgggtgtcg gttcaggcgt ggtccccggt gaggggatgc
     1741 tttcagcggt acgggggggt tccgcgggaa tcgaagtcga agaggtggcc ggagattcca
     1801 cgacggcaac aggggccgag ggtgttgcgc tcaccgaaac ggtcgcgccg gccatcacgg
     1861 ggtcggtgtt atcggagcgc tgcgaaatca atgcagccaa ggcgacgatg agcgcggaaa
     1921 gaagcaccgc gattcccacc agaagtcctt tgcgttgtgg ctgtttgttg gccgtaacca
     1981 ccgcgcccga accggaagca ggggagaacg acgatgaggt catcgactcg gcgagtatgt
     2041 ttctgcgctc tcggggacga ggagtagcgc cggaggacgc gagggaatgg gattcgatgg
     2101 tgcgtcggat tcgctcgatg ctgacaagca cttgatgagg gacaagggcc ccgacggcat
     2161 cgattacgtc ttcagcgaat tccgcaacgt ttggggtgcg acgattgagg tctctttcca
     2221 gggctcggaa aatggcggct tcgagttcag gggggacgtc gggtctgcgt gcgcatagag
     2281 aggggggcaa ttcggttcca agaacacgca tgcatgtggc agcgtgggta tcggcgacga
     2341 agggaggcgt gcccgctagc atttcataca gcacgacccc gagggaccaa acgtccgaac
     2401 gttcatcgac ttgcgcagta ttggtcaact gttcgggaga catatacttc ggcgagccaa
     2461 gcaaggctcc ggcggcagtc aagtcttcaa ggtcagaaga ggagaccaag ttggccttgg
     2521 agataccgaa gtcgaggact ttgatgagtt cggtaccatc gcgacgtgtg gtgacaaata
     2581 ggttggaagg tttgagatcg cggtggacaa cggaaaagga gtgcgcttcc gcaagtcctt
     2641 cacaagcctg aatgatgtaa tcaatggcgc gagggatggc caggggcccc tcttttttca
     2701 gcatggaacg cagatcgaca ccggagatat actccatgac catatagggg gctccgcttt
     2761 cgagcattcc gacatcggat actttcacga tgtgctcatt gcggagtttg gcgctgactt
     2821 gtgcttcacg catgaagcgc gcattgaatt cttcggagcg gcccgcaccg gggcgcaaga
     2881 atttcagtgc gacacgttct tgcaggtgca aatgcatggc ggccacgacg acccccatgc
     2941 cgccttgccc caagatgctt tcgactttgt actttccgac cagtacatcc ccgatatcga
     3001 cggatacagc gccatgctca ccggaagatg cgagcacccc tgtcgtttga gttccgtccc
     3061 ttgccatttg atctcgctat tcggttggtt gttccacggt ggccaggtca atacgttcat
     3121 tcaagccatc cgcgagcccc gcagacgtag ccagtataaa gattatctca ggcacgaatc
     3181 cttggcaatg ttcacgcatg ccgacccttc agggtgataa ccgaatggca gctcgcattc
     3241 catagtggtc cgaaacaggg acccacaatt cctcaccttc gttttcaata cgaatagtat
     3301 tatccagtaa cgttctctgc gcggagagaa tggattggtc tgaaataccg tgtagaaaga
     3361 tatgatctac ccaggcggat tcccattctt tcgtcagtcc ggtcagcacg ttggaggaac
     3421 agaatgtgca tgctggcgga taatcagggg gaatggcttc cgtatagtgt tgttggagca
     3481 aggtcacggt ttctggccct tcggctcggg tcaccattct gccattttcg cggaattcga
     3541 gactggcgtt gaaatcgccg agaacgatgg cttgacgatc cgcggagtac ttcaacaacc
     3601 atgcatgaag cttttccgct tgtagctttt gctctgcagc ccaggcatcg ggtccggtct
     3661 taccgtcccc gtagtctccg gtgtatggga aagccacatc gaaaaacacg ggagccaagt
     3721 gattgcaata cacatcgact tccccggcgg ggagtttggc ggtagcgcgc aggatgacac
     3781 gacggttcca ggtcgccgga agaacccaat cttccgcgtt tatcaaaggg tgtcgggaaa
     3841 ggatcatgac accgctttgt ccatcgaagg ccagggcggc tttggggttg gttgtgcatt
     3901 tttcttcgat cctgcctaga gtatcctcgg gaaggctagt gaccgcgcag ccgtagcagc
     3961 gcttgtcttg ctcggcgcca gccaacaagg aagccgcttc gacaaaacac ttctcttccg
     4021 cgcaggtcgc cgtcgttact ttgccctctc gttgccccgg caaggtggaa caattatcca
     4081 gcaaacactg taataccgct tcgaacttct cttttgtccc ttggacccca caaggcggct
     4141 cggtaggggc cggtggtacc atgccatggg catctgccgg atcgtccact tcggtatcga
     4201 aatcgtgctc gaaccagact gcataaggga aattcgcttt cgcgatttcc gccaacttct
     4261 ccttatccct tttccgccaa acctcttgaa cgcacaccac atcagaattg agcgacgcca
     4321 ccgcttccat cacaggctga agccgttgtt gctcgaaggg aacaaatgac ccagctaagc
     4381 caatattgaa cgtatcaata atataggtgt cctggattgg ggattgttca tcattttcat
     4441 cactcgaaca cgaagccaga gcgagtccga acaacccggc aataaggatt tgatgaatca
     4501 cgcggcgcat cattcctcca tgttcgagcg cgacaggagc ctccttcgtc gttcgaacca
     4561 catacgattg tcaccaaggt taggtttcag aatctggcgc atgccagcta caggatcaag
     4621 gtcagcgtca caattagatc ataattggaa atcatttcgt tacggaacca tacccctggc
     4681 atttctgcca tactctcatt gcaactctgc aattcattca acataacccc gaggtaacta
     4741 gccgtatttc ctatttatgt aggtggcgca tctgttgcga cgccagccca ggcgctatgc
     4801 atcccccttc gcagccaatc gctgacccgc cttcggctgg gaaaccgcct ttcgcagaac
     4861 cggttggcct gcatttacgt cgtgcgcgcc ttcagtgtgg cttgacgctt caagatattt
     4921 ctcgcaccac acgaatttcg gtctctcaac tcgactatct cgaacgggac cgcttcgagg
     4981 atttgcccgg caatctgtat acccgcggat tcattcgttc ttttgcaaaa gcggtagggc
     5041 tcgatggcga gcttgccgtg gcgcattaca cctcgaaccg tcgctgccga tccataagcc
     5101 tcttcccagc ccaaaaccat gtcgaaaaaa agcgtcgagg cttgggaatt tttgtggcct
     5161 gctccgcctt tttgctgctg atcgtcgtag caaccctggc ggtttcccat gcgaagccca
     5221 aaaatgcagg gcgcctcctg tccttttccg cccttaccca cccaggcgaa agaccgccgt
     5281 cgttggcttg accggctctt ttccgacgcc tatgttcggc gcccccgtgt ggctactctc
     5341 gagccatttc gcgaattatc tagcttcgcg ctcttggttg cccacggcaa agcgagtccg
     5401 cacagagtcg aatcatgagc gaacgatccg agttacagcg agaaatcgat aaaagaagga
     5461 ctttcgccat catctcccac ccagacgctg gcaagaccac tcttacggaa aaattattgc
     5521 tttacggtgg ggtggttcat ttggccggtg cggtcaaagc gaaacgggga cgcgccaatg
     5581 ccgtcagtga ttggatggaa atggagcgag aacgcggaat ctccatcacg acgagcgtat
     5641 tgcagttcga atacaaggga cttcagctca accttctgga tacccctggg cacgcggatt
     5701 tcagtgaaga tacctaccga acgctgcacg cggtcgatgg ggcggtcatg ttgctcgacg
     5761 ccgcgaaagg tgtggaatca caaacgcgca agctatttcg ggtatgtcgt cagcgttcga
     5821 ttccgatttt cacattcgtc aacaagatgg atcgtccggg gcgggatccg ttcgaactc
//
LOCUS       DAOSOR010000369         5870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13744_length_5870_cov_4.014101, whole genome shotgun sequence.
ACCESSION   DAOSOR010000369 DAOSOR010000000
VERSION     DAOSOR010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13744_length_5870_cov_4.014101"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="PK710_17375"
     CDS             <1..1160
                     /locus_tag="PK710_17375"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOT11553.1"
                     /translation="GADSFHAITGKPLSLTPSISKILWVRRSEPDFFQRIERWLDVHA
                     WLVRHFTGQDVTAVGSADPMGLIDISLGQWDDGLLAVAGLDQRSVPRLVPSGHIVGKL
                     TNQHASVMGFPFPIPIVATAGDGQVAALGAGIRDLRTAYMNLGTAIVAGVVSRTAWKD
                     LAFRTMVGAESGTYLLESDLKGGTFCIDWLSHKLLNGRVGVPQLEQNAASIRPGSEGL
                     MWVPYLATAMNPYWDDNASGLVVGLRGDHGPAHIFRAILEGIAFEQRLQLEGIERVSH
                     ERIEIVRVLGGGAQSDLWCQILADVLNRPVVRTKQPETTSLGAAMLAAITLSALPSLS
                     EAIHEMSKEDKAFEPGTSKDLYARLYSEVYSHLYPSVRTSMSNLTKILRNP"
     gene            1229..3523
                     /locus_tag="PK710_17380"
     CDS             1229..3523
                     /locus_tag="PK710_17380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11554.1"
                     /translation="MRQRLFRRYRWLKGIAVVLTILFVGCDDEADGAKDTGSGLGGSA
                     GSGGSGGGHGGTGSAGTVPTEPVTLDPSDFTYQLEESREDLVLWTTPATHKLRTIDRP
                     APTIRSGLHLSAARREFEPVQLIVGPASGTVSAHVEPFSTLGSGQRVQLSVVRYENGW
                     GEYLDPVTGAMPLSEGYGLGLWLTVYVPDDASAGDHTTTLTLTPNHGSPIVVPITLHV
                     FDFAIPETIHFASQLNVNISALIPEGGKEQVVKDMLFEHRLTPKSVTWPSGFNWNITW
                     DSSASPNRCEIFFDEPDEGEAYSIQHLARKYIHGQGWNGIGFPNAMIFQFVDNATPRP
                     DTLCGIARGDHLGTVAYNNEWSQFLSALDTYLVSHGYEQKAYYYVQNEPQNDADHALA
                     NHLCRLTKAAAPHLRIAVSDEPKPEIAEHPNGPCGYDIWIAHIREYKESYAWKRQREY
                     GEKVWFYSLDHDPDPYFNPTRVDVQGMHQRIIPWAAWVHRITGWAYYDGGRFFDGSRP
                     TIRAELLREGFEDYEYLFLANGGNHPRVDETSSVDATVKSVASSMSSWNDDADALMVL
                     RHELGRYLEGSRDTLPVLEAQGKAHPRASYYLNFQDPAGPPTAEPLVVDGKTYRKVGW
                     SPWDDEQAFGWFGENIGNSEILRSGYDDVAGFNEVERSYIYDDYGRNNRFEFGLENGV
                     YEITIGVGRARRGYPHDPHTAIIEGTRVIDDEVTTEEAPMIIRKQKVTLMDGKLTLEV
                     GGKSPSTNDWAYTFLSFLWIEPTQ"
     gene            3615..4268
                     /locus_tag="PK710_17385"
     CDS             3615..4268
                     /locus_tag="PK710_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOT11555.1"
                     /translation="MKEGVVEPAETKPSIRHFSLIRDFTVADFITLANASSGMGSVLA
                     VMQHLVTGESKYLWVAFALLPFALVFDYLDGRIARWRCMSSLLGTQLDSLSDLISFGV
                     APASLAFAVGMRGGWDALVLLYFVACGVGRLARYNATCADLSDETGKVRYFEGTPIPT
                     SLLLVAVLSVLTAYGYTLDRLPLGVWTLGPMQLHPLVLMYAVSGSAMISKTLRVPKI"
     gene            complement(4237..5337)
                     /locus_tag="PK710_17390"
     CDS             complement(4237..5337)
                     /locus_tag="PK710_17390"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT11556.1"
                     /translation="MSEPSLLSVLPEDLITRFPSLEMSEARRLLSIVHRTGSLPPHAP
                     AGIRRDSFQRVRDAVPMHQLERVTRRPSALDPFIKYGFRTLDGLLVETVRIPLEKPGR
                     FVVCVSSQTGCALGCAFCATARLGTGRNLAAWEIVDQVRQVRDDLATDGRVHGVVFQG
                     MGEPLANVSAVVQAIRVMTDPSLQAIDGRAITVSSAGLIKPLPVLLQALPRTRIGISI
                     GHADPIKRRALMPVEVANPLHNVLAIVAQHAKTTRIATMLSYTLLREINDHESDADAF
                     LALVDAYVQQAGMAPRISLIAYNPIGPSDPFVPSEPEQAERFRERLASHGIPVVRRYS
                     GGADVGAACGQLGLESVMDPTHVKSSEHEASC"
     gene            5488..>5870
                     /gene="tatB"
                     /locus_tag="PK710_17395"
     CDS             5488..>5870
                     /gene="tatB"
                     /locus_tag="PK710_17395"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HOT11557.1"
                     /translation="MLGLSFTEIVVVLLVAVVVIGPRQLPVMLRTAGRWIAKLRRMAF
                     DMRTQSGIDDILRKEGLDKDLRQLQALMRRGNVLDALAIDVDAEIAREKPSKTDSERL
                     PLREPDDLDMRDREYPVAGVDAYGLG"
BASE COUNT         1256 a         1547 c         1738 g         1329 t
ORIGIN      
        1 tgggcgctga ttcctttcat gccataaccg gcaagcctct atctctcact ccttccatct
       61 cgaaaattct ctgggtgcga aggtcggaac ccgatttttt tcaaagaatt gaacgctggc
      121 tcgacgttca tgcttggctc gttcgacact tcacgggaca ggacgtgacg gccgtaggtt
      181 ccgcggatcc gatgggactc atcgatattt ctctcgggca gtgggacgat ggcctgttgg
      241 cggtcgccgg actcgaccag cgatcggtgc cccgattggt tccttcgggc catatcgtgg
      301 gaaagttgac gaaccaacac gcttccgtga tgggatttcc ctttcccatt cccatcgtgg
      361 cgactgcggg ggacggacaa gtagcagcgc ttggggctgg tatccgggac ttgcgcacgg
      421 cgtatatgaa tctgggtacg gccatcgttg cgggcgtggt gtcacgaacg gcttggaagg
      481 accttgcgtt tcgcacgatg gtcggggcgg agtcagggac atatttgttg gaaagcgact
      541 tgaagggcgg aacgttctgt atcgattggc tgagccacaa gctgttgaac gggcgtgttg
      601 gtgttccaca gctcgaacaa aatgcagcat cgattcgccc cggttccgaa gggctgatgt
      661 gggtgcccta cctcgcgaca gctatgaatc cctattggga tgataatgcg agcgggcttg
      721 tcgtgggact gcgcggcgac cacggaccag cccatatctt tcgggccatt ctcgaaggaa
      781 tcgccttcga acaacgtctt caactcgagg ggatcgaacg ggtttcccac gagcggatag
      841 agatagttcg cgtccttggc ggcggcgcac aaagcgattt atggtgtcaa attctggcag
      901 atgtactgaa tcgaccggtc gtacgaacga aacaaccaga gacaacatcg ttgggagcgg
      961 ccatgcttgc ggctatcacc ctttccgctc ttccctcgct ttcggaagcc atccacgaaa
     1021 tgagcaaaga ggacaaggcc ttcgagcccg gaaccagcaa agatctttac gcacggttgt
     1081 actccgaggt ctattcgcac ctctaccctt ccgttcgaac gtcgatgtcc aacctgacga
     1141 agatccttcg taacccttga tccgtcgtcc ccatcgcctt cttgttcgcc ctacggaaga
     1201 cacatagtct cgtgctaccc tagtcgacat gagacagcgg ctatttcgtc gttatcggtg
     1261 gctcaaaggg atcgctgtcg ttctcaccat ccttttcgtt ggttgtgacg atgaggctga
     1321 tggagcgaaa gacaccggat ctgggttggg aggatcggca ggcagtggtg ggtcgggagg
     1381 aggtcatggt ggaacaggtt cggcggggac cgtacccacg gagccggtaa cgctcgaccc
     1441 ctcggatttt acctaccagc tcgaagaaag cagggaggac cttgtgctgt ggacgactcc
     1501 ggccacccac aagcttcgaa cgatcgatcg tcctgctccc acgatccggt cggggctgca
     1561 tttgagcgcg gctcgtcggg aattcgaacc cgttcaactc atcgtgggcc cagccagcgg
     1621 cacggtatcg gcgcatgtcg agccgttttc aactcttggt tctggacaac gggtacagct
     1681 ttcggtcgtt cgttacgaaa acggttgggg cgagtacctc gatccggtca ccggtgccat
     1741 gccgctgtcc gaagggtacg gccttggcct ctggcttacc gtatatgtgc ccgacgatgc
     1801 ttccgcgggc gaccacacga ctacgctcac gctgacacca aatcatggct cgccgatcgt
     1861 ggtaccgatt acgctccatg tatttgattt tgccattccg gaaacgattc actttgcgag
     1921 ccagctcaat gtcaacatca gcgcgttgat tcccgaaggc ggcaaggaac aagtcgtcaa
     1981 agatatgctg ttcgagcatc gattgacgcc aaaaagcgtg acgtggccga gcggtttcaa
     2041 ctggaacatc acatgggatt catctgcctc acccaaccgc tgcgagattt tttttgatga
     2101 gccggacgag ggagaggcgt attccatcca gcatcttgct agaaaataca ttcatggaca
     2161 gggatggaat gggattggat ttcccaatgc catgatcttt caattcgtgg acaacgccac
     2221 acctcgtccc gacacactct gcggcattgc tcgtggcgat catcttggaa ccgtggcgta
     2281 caacaacgag tggtctcagt ttctctccgc tctcgacacc tatctcgtca gtcacggata
     2341 cgaacaaaaa gcctattatt acgtgcaaaa tgaaccgcaa aacgacgcgg accatgctct
     2401 tgccaaccat ctatgccgtt tgacgaaagc ggcggcgcct catttgcgca ttgcggtgag
     2461 tgacgaaccc aaacccgaga tcgccgaaca ccccaacggt ccctgcggat acgatatttg
     2521 gatcgcgcac atacgagaat acaaagaatc ctacgcgtgg aagcggcaac gcgagtacgg
     2581 agaaaaggta tggttctaca gtttggacca cgatcccgat ccatatttca atccaacgcg
     2641 agtcgatgtt cagggtatgc atcagcggat cattccgtgg gcggcgtggg ttcatcggat
     2701 caccggctgg gcgtattatg atggtgggag gtttttcgac ggctctcggc ctacgattcg
     2761 cgccgaacta ttgcgagaag ggttcgagga ttacgagtac ttatttcttg ccaatggcgg
     2821 caatcacccc agggtagatg aaacgagcag cgtcgatgcc acggtcaaaa gtgtggcttc
     2881 gagcatgtcg agttggaacg acgatgctga cgcgttgatg gtgctgcgtc atgaactcgg
     2941 gcgctatctc gagggatcgc gcgatacgct ccccgtgctt gaagcacaag gaaaagcgca
     3001 tccacgagct tcgtactatc tcaacttcca ggatccggcg ggcccaccca ctgccgagcc
     3061 attggtggtg gatggaaaaa cttatcggaa ggtcggttgg agtccttggg acgacgagca
     3121 agcgttcggc tggtttggag agaacatcgg caatagcgaa atcctccggt cgggctatga
     3181 cgatgtagcg ggcttcaacg aagtcgaacg aagctatatt tacgatgact acgggcgaaa
     3241 caaccgcttc gaattcggtc tggaaaacgg tgtctacgaa atcacgatag gggtaggtcg
     3301 tgcgcggcga ggttatccgc acgatccgca tacggcgatc atcgagggga cacgagtcat
     3361 cgacgatgaa gtcacgacgg aggaggctcc gatgatcatt cgcaagcaga aggtgacgtt
     3421 gatggatggc aagctgacgc tggaagtggg aggcaagtca ccgagcacca atgattgggc
     3481 ttatactttc ctttcttttt tgtggatcga gcccacgcag tgatcctcgc tttcgttcac
     3541 catgtccgaa gccagtggat gccaacaatg gctggtgata ggttcgtttt ccttcgaaaa
     3601 tggatcgtgc gaacgtgaag gagggtgtgg tggaaccagc ggaaacaaaa ccatccattc
     3661 ggcacttctc gctcattcgg gacttcacgg tcgcggactt catcaccttg gcgaatgcgt
     3721 catcgggtat gggctcggtt cttgccgtga tgcagcacct cgtcacgggg gaatcgaaat
     3781 acttgtgggt cgcctttgcg cttctgcctt tcgcgttggt atttgattat ctcgacggtc
     3841 ggattgcacg atggcgttgc atgtcttccc tattgggaac tcaactcgat tctctttctg
     3901 atttgatctc ctttggcgta gcgcccgcgt cgttggcatt tgccgtgggc atgcgtggcg
     3961 ggtgggatgc gctcgtattg ctgtattttg ttgcgtgcgg cgtgggtcgt ctggcgagat
     4021 ataacgccac ctgtgccgac ctatccgacg agacgggtaa ggtgcggtat ttcgaaggga
     4081 cgccgattcc gacgagtcta cttctcgtcg cggtgctctc tgtgctcacc gcatacggat
     4141 atacattgga tcgtttaccg ctgggcgtgt ggaccttggg tccgatgcag cttcatccct
     4201 tggtcttgat gtatgcggtg agtggatcgg ccatgatcag caagacgctt cgtgttccga
     4261 agatttgaca tgggttgggt ccatgacgct ttccagccct aattggccgc aggctgcccc
     4321 gacatccgca cctccggaat aacggcgaac cacgggaatg ccgtgggaag cgagacgttc
     4381 tcgaaaccgt tcggcttgct cgggttcact aggaacaaat gggtcggaag gtcctatggg
     4441 gttataggcg atcaagctta ttcgtggcgc catccctgct tgctgaacgt acgcgtcgac
     4501 gagcgcaagg aaagcgtccg catcgctttc atgatcattg atttcccgca acaaggtgta
     4561 ggaaagcatg gtcgcgatac gtgttgtttt tgcgtgttgc gcaacgattg caagcacatt
     4621 gtgcagtgga ttggcgactt ccacgggcat cagagcacgg cgtttgatcg gatcggcatg
     4681 tccgatggaa atgccgattc ttgttcgcgg caatgcttgc aacaatacgg gcaaaggttt
     4741 gatcaatcct gcggagctta cggtgatcgc tcgcccatcg atcgcctgaa ggcttggatc
     4801 ggtcatgaca cgaatggctt gaacgactgc actaacgttg gcgaggggct cgcccatgcc
     4861 ttggaagacg accccgtgga ctcgtccatc ggtggcaagg tcatcgcgaa cttgacgaac
     4921 ttgatcgacg atttcccagg cggcaagatt tctgcccgtg ccgagtctgg cggtggcgca
     4981 aaaggcgcaa ccgagagcac agccagtttg cgacgataca caaacgacga aacggcctgg
     5041 tttctcgagg ggaatacgaa ccgtctccac cagcaggcca tctagcgttc gaaatccata
     5101 tttgatgaac ggatcaagcg cacttggacg tcgtgtcacc cgctcgagct ggtgcatggg
     5161 aacggcatcg cggacgcgtt ggaacgaatc ccgtcgaatc cccgcgggag catggggggg
     5221 caacgagccc gtgcgatgaa cgatagacag caaacgccgg gcctcggaca tctcgaggct
     5281 tggaaaacgc gtaattaaat cctcgggcaa caccgacagc aaagaaggtt ccgacacgac
     5341 gtcggcaacc atagcgcaat cggcgcaatg ggcggagcgg atccttgggt ccaaggcgcc
     5401 gttgatttca ggtgcctgga cagcagctct tggctgattg ccaaatcatc tttgcgacta
     5461 gggccatcgc aagctagctt gccaccggtg ctcggtctgt cgttcacaga aatcgtggtg
     5521 gttcttctgg ttgccgtcgt ggtcattggt ccacgccagc tcccagtcat gcttagaacg
     5581 gcagggcgtt ggatcgcgaa gttgcgacgg atggctttcg acatgcgtac gcaaagcggg
     5641 atcgacgata ttttgcgcaa ggaggggctc gacaaggacc ttcgtcagct acaagcgttg
     5701 atgcgtaggg gcaacgtgct cgatgcgtta gccatcgatg tggatgcgga gattgcgcga
     5761 gaaaaacctt caaaaacgga ttctgaaagg ttgccgttgc gcgagcccga cgacctggac
     5821 atgcgggatc gggagtatcc cgtggcaggg gtggacgctt atggcctcgg
//
LOCUS       DAOSOR010000370         5839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13831_length_5839_cov_4.721300, whole genome shotgun sequence.
ACCESSION   DAOSOR010000370 DAOSOR010000000
VERSION     DAOSOR010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13831_length_5839_cov_4.721300"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1570
                     /locus_tag="PK710_17400"
     CDS             <1..1570
                     /locus_tag="PK710_17400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11558.1"
                     /translation="LGMVLLGAPVGLAGVALSLRAPQSTGLLILVVGGLSILVGLVAP
                     ALSRPLGPARSRWLQAIQKANEAALHPDPDVALRDSLTTVRSLLPGDSASPTIFQASP
                     PQMLTIDRAGYLHTHEIQAPMTLYDIAQAEPERAIRTEVLRAMEVRRAELRPLVQWMD
                     ARGMLSVTLIRDEDGPVGLLGIPRGKRRTPMSLEEVRAVRVLADRIGAVLGVSSALAR
                     SRQRELELRQLAERRADEIDRLRFQLSAKSGRMRAHAERLALPLRATTYSPAAAMALE
                     QVRRLGAMDIPVTLLTPPGVDPIAWAAEAHLASSHASSPFVIVYGTDTSEHDIDRWRA
                     ADTSPLSLADGGTLFVIDIAALPRTVQDYIASCLAERIAPGQVTPLDIVLMVSVPATA
                     DVLVAAARLSSTLADWLGDRAVPLPELAARPEDLRALVLDQLARAGTRLRGAPLGIDD
                     RALARLMEHQWPGNDVELADVLLRATLVAQEKRVTLEDLQTIGFIIESSNAESSEPPP
                     SLAPTIPKPRRRRH"
     gene            complement(1624..3075)
                     /gene="glgA"
                     /locus_tag="PK710_17405"
     CDS             complement(1624..3075)
                     /gene="glgA"
                     /locus_tag="PK710_17405"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HOT11559.1"
                     /translation="MSQRNILYVTSECTPFAQVGGLGDVASALPAALRRAGYDIRVVM
                     PLYGEVDRDALHPIGVSLRIGLADTVFDARVWQGELPERVPLYLIDQPDLYARSGVYG
                     PKGGAFEDNLLRFAWLSRAACDLPRHVGFAPHVLHANDWPTALSILFAKHLSQPVPTV
                     FTLHNVGYQGIAPLHDFALTGLPSRELSADSLEHFGAINLLKGGILGASMLTTVSPTY
                     AKEIQHPAFGCGLDGVLRSRASDLLGILNGIDDKAWNPSIDPWIPSPYDADNLSGKAI
                     CKSVLQREAGFAVRPEVPLFGLVTRLTHQKGLDVLAHALGRFLSMDVQMVLLGTGDAP
                     AEAFFEMASRQRPDRFHAWLGFDPALAHRIEAGCDFFVMPSRYEPCGLNQMYSLRYGT
                     LPIVRATGGLEDSVDNYDEATGQGTGFKLYDLNPPSLLNTLAWAVSTWVERRTHIETM
                     RRRAMRQDFSWFRSAKGYETVYEKAIASSRQQR"
     gene            complement(3155..4738)
                     /locus_tag="PK710_17410"
     CDS             complement(3155..4738)
                     /locus_tag="PK710_17410"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOT11560.1"
                     /translation="MAHGYLALVLHAHLPFVRHPEAPSYMEEEWLFEAITETYVPLLL
                     VFERLLRDDVDYRLTLSMSPTLLSMLTDDLLKERYATKIDKLVELAESEVDRTRNDGR
                     FHGLAQMYRDRFVRIRDYWRRYDGDLVHPLRGLQDAGKLEVITCTATHPFIPFMDRNW
                     AAIRAQVHVAADVYERAFGRRTQGMWLGECGYVPGLDELLREENIRYFFLDTHGLLFA
                     DRRPVYGVHAPIYCPTGVAAFGRDVPSSRQVWSSKEGYPGDPDYRDFYRDIGFDLPIE
                     YVRPYIHPDGIRTFTGVKYHAITHGALHNKNPYVPHVAWQRANAHAQHFLEQREQQVR
                     ALRPQMDRPPIIVSPYDAELFGHWWYEGPIFLEMLLRKIHTDQGMIAPITPSAYLHAH
                     PTNQVATPAMSSWGAGGYGEYWCNQSNAWIYRHLHKMGERMVELARRFSQADDLQRRV
                     LNQAARELMLAQASDWAFIMKSGTTVAYATQRTNDHINRFHRLTQNLEDGDIDEDYLA
                     EVERRDNLFPNLDYRVFAT"
     gene            complement(4738..>5839)
                     /locus_tag="PK710_17415"
     CDS             complement(4738..>5839)
                     /locus_tag="PK710_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11561.1"
                     /translation="WMTESWRTEWQGDERAFEWIRPLHNVAWSREQVLTTWDWAPYPG
                     DSAAPGRVLVRFLGVDYTYMDSGFEKESAGSWEIVIHGASFGTSFRRVLGTWRLHRAQ
                     TIESVVERWESIVERIWHEGFVRERTWTGASEMRQDVEQGASERWILGGSERISYAIE
                     EQLALGGSEWIWLGASELRLAGASERVTEWQFAGLGASEWVLEQGSERVFIGASEQLR
                     MGASEQLRMGASESSWQASEVFAQASESFLHDYGGASESEKRKWWVDLGGSIGASELL
                     GMRESTRFIKPTLPFAGASEQTALGSALMPHPPSQDTISDSVSMAELGIHALAFDDRS
                     WTAVGASERVAQATYPATLDDDDENESSKGGG"
BASE COUNT         1238 a         1766 c         1641 g         1194 t
ORIGIN      
        1 tttgggaatg gttcttctcg gtgctcccgt gggcttggcg ggcgtggcct tgagtttgcg
       61 cgctccccaa tcgaccggac tgctcatttt ggttgtcgga ggcttgtcga tccttgtggg
      121 attggtggct ccagcgctgt cgagaccgct aggaccggca cgctcccgtt ggcttcaggc
      181 cattcaaaaa gccaatgaag cggctttgca tcctgatccg gacgtggccc ttcgagactc
      241 gctcaccacc gtacgctcct tgttgccggg ggacagcgca tcgcccacga tttttcaggc
      301 ctccccaccg cagatgctca cgatcgaccg ggccggttat ctgcatacgc acgaaatcca
      361 ggctccgatg accttgtatg acattgcgca agcagagccc gagcgggcga ttcgtacgga
      421 agtgcttcga gcgatggagg tgcgacgcgc cgagcttcgc cccttggtcc aatggatgga
      481 cgcaagggga atgcttagtg taacgctcat ccgcgacgag gatggcccgg tcggccttct
      541 cggaatccca cgcggcaagc gtcggacccc catgagtttg gaagaagttc gcgccgtgcg
      601 ggtgctggcg gaccgcatcg gtgccgtgct tggcgtttcc tcggcgctcg cccgatctcg
      661 gcaacgggaa ctggaactgc ggcaactggc ggaacgacgg gcggacgaga tcgatcggct
      721 acggtttcaa ttgtctgcga agagcgggcg aatgcgcgcc catgccgaac ggctcgcctt
      781 gcctttgcgc gccaccacct atagcccggc ggcggccatg gcgctcgagc aggtccgacg
      841 actgggagcg atggacattc ccgtgacgtt gctgactccc cccggagtcg atcccattgc
      901 ctgggccgcg gaagcccatc tggcgagttc ccacgcatcc agcccttttg tgattgtgta
      961 cggtaccgac acttccgaac acgatatcga ccgctggcga gccgccgaca cttcgccttt
     1021 gtctcttgcg gatggtggaa ccttgttcgt gatcgacatc gccgcccttc ctcgaaccgt
     1081 gcaagactac atcgcttctt gcctggccga gcggatcgca ccaggccaag tcacaccgct
     1141 ggatatcgtt ctcatggtat ccgttcccgc aaccgcggat gtgctcgtcg ccgcggccag
     1201 actctcgtcc acactggcgg attggcttgg agaccgcgcc gtgccgctgc cggaactcgc
     1261 cgcacgcccc gaggacctgc gtgcgctcgt gctcgatcaa ctcgcgcggg cgggtacacg
     1321 tcttcgcgga gccccgcttg gcatcgacga tcgcgcgctc gcgcgattga tggaacacca
     1381 atggcccgga aacgatgtcg aacttgccga cgttctgctt cgcgccaccc tggtagccca
     1441 ggagaagaga gtgacgctgg aggatctgca aactattgga tttattatag aatcttcgaa
     1501 tgcggaatca tcggagccgc cgccatccct cgcaccaacc atccccaaac cgcgtcgaag
     1561 acgccattga ccgctttcgt ttcgtcgtcc gaatacgccc ccgccatctc gagatgcggt
     1621 cgctcagcgt tgctgcctgg aagacgcgat cgctttttcg tacaccgttt catagccttt
     1681 cgccgaacga aaccaggaaa aatcctgtct catagcccgg cgacgcatcg tttcgatatg
     1741 ggttcgtcgc tccacccagg tggatacggc ccaggcgagg gtgttgagaa gcgatggggg
     1801 attgaggtcg tagagtttga atccagttcc ttgccccgtc gcttcgtcgt agttgtccac
     1861 ggagtcctcg aggccaccgg tggcgcgaac gatcggcagg gtaccgtacc gcaagctata
     1921 catttggttc agcccacaag gttcgtagcg cgatggcatg acgaaaaagt cgcagccggc
     1981 ttcgatgcgg tgggcgaggg ccggatcgaa tccaagccat gcatgaaatc ggtcagggcg
     2041 ctgtcgtgag gccatttcga aaaaagcttc cgcgggtgca tcgccggtgc ccagcaacac
     2101 catctgcacg tccatggata gaaagcgtcc aagggcatga gccagcacgt ccaacccctt
     2161 ttgatgggtc agacgtgtga cgagtccgaa caagggaacc tccggtcgta cggcaaaacc
     2221 tgcctctcgt tgcaagacgg acttgcagat tgccttgccc gacaggttat ccgcatcata
     2281 gggcgatgga atccacgggt cgatggacgg gttccatgcc ttgtcatcta tcccgttcag
     2341 gatacccaac agatcggatg cgcgggaccg caacacacca tcgagtccgc atccgaaagc
     2401 ggggtgttgg atctctttcg catacgttgg gctcacggtc gtgagcatgg aagcgccaag
     2461 gatgccgcct ttgagcaggt tgatcgctcc gaagtgctcg aggctgtcgg cgctgagttc
     2521 tcgggaaggc aaaccggtca aggcaaaatc atgcaacggc gcgatgccct ggtatccgac
     2581 attgtgcaag gtaaataccg tcggcacagg ttgggataag tgcttcgcaa acagaatgga
     2641 aagtgccgtc ggccaatcat tggcatgaag gacgtgaggc gcgaagccga catggcgcgg
     2701 caggtcgcaa gcggctcgac tcaaccaagc gaaacgcaaa agattatcct cgaaagcgcc
     2761 gcctttgggt ccatacacac ccgatcgagc atataaatcc ggctgatcga tcaagtacag
     2821 aggcactcgt tccggcagtt ccccttgcca aacgcgagca tcgaataccg tatcggccaa
     2881 tccaattcgc aaagacaccc caataggatg caaagcgtct cgatccactt cgccatacaa
     2941 aggcatcacg acccgtatgt cgtagcccgc tcgacgaagg gcagcgggca aggccgaagc
     3001 gacatctccg agccctccta cttgggcgaa gggcgtacat tcggacgtta cgtacaagat
     3061 attacgttgg ctcatcgcga tcatgaacgt cgctggaagc gcttctcgaa ccaggcgaag
     3121 attccttgga aatagcttgt ttcgtatcta tcgatcacgt tgcgaacacg cggtaatcga
     3181 ggttggggaa gagattgtcg cgacgttcca cctcagcgag ataatcttca tcgatatcgc
     3241 catcttcgag gttttgggtg agtcggtgga atcggttgat atggtcattg gtacgctgcg
     3301 tggcgtaagc gacggtggtg ccacttttca tgatgaaagc ccaatcgctg gcttgtgcga
     3361 gcatgagttc acgagcggct tgattgagga cacgacgttg caagtcgtcg gcctgggaga
     3421 aacggcgggc gagttcgacc atgcgttcgc ccattttgtg aagatggcga taaatccacg
     3481 cattggattg gttgcaccaa tactcgccgt agccccccgc accccaggaa gacatcgctg
     3541 gcgttgcgac ctgattggtg ggatgcgcgt gcaaataagc actgggtgtg atgggtgcga
     3601 tcatgccctg atcggtgtgg atttttcgca acagcatttc gaggaagatg ggaccttcgt
     3661 accaccaatg gccaaatagc tcggcgtcat aaggagaaac gatgatggga ggccggtcca
     3721 tctgaggacg taacgcgcgc acttgttgct ctcgctgttc gagaaagtgc tgcgcatgcg
     3781 cgttggctcg ctgccatgcc acatgcggaa catacgggtt tttattgtgc aaagcgccgt
     3841 gggtaatggc gtgatacttg acccccgtga acgtccgtat gccatcggga tggatgtagg
     3901 gtcgcacgta ttcaatcggc agatcgaaac cgatgtctcg ataaaagtct cgatagtccg
     3961 ggtcacccgg atacccttcc ttcgaactcc acacctgccg cgaactcgga acgtcgcgac
     4021 caaaagcagc cacacccgtg ggacaataga tcggggcgtg aacgccgtag accggacggc
     4081 gatcggcgaa gagcagtcca tgggtatcca ggaaaaaata gcggatattt tcttctcgca
     4141 gcagttcgtc caaacccggc acgtatccgc attccccaag ccacattcct tgcgtcctgc
     4201 gcccaaaagc acgttcatat acatccgccg ccacatgcac ctgggctcga atcgccgccc
     4261 aatttcgatc catgaaagga atgaacggat gggtcgccgt acacgtgatg acctccagct
     4321 tccccgcatc ctgcaaccca cgaagcggat gcacgagatc gccatcatag cgtcgccaat
     4381 agtctcggat tcgcacgaat cgatcccgat acatctgcgc aagtccgtgg aaacgaccgt
     4441 cgttacgggt tcgatccacc tcactttccg caagctccac aagcttgtcg atcttcgtcg
     4501 cataccgttc tttgagaaga tcatcggtga gcatgctcaa cagcgtgggg ctcatcgaaa
     4561 gggtcagacg atagtccacg tcatcgcgca gcagccgttc gaaaacgagc agcaacggaa
     4621 catacgtttc cgtgatggcc tcgaacagcc attcttcttc catataggaa ggggcctcgg
     4681 gatgacgcac gaacggcaag tgcgcgtgca acacaagcgc caaatacccg tgtgccatca
     4741 cccacctccc ttggacgatt cgttttcgtc gtcatcatca agcgtggctg ggtaggtagc
     4801 ctgcgccacc cgttcactgg cacccacagc cgtccagctt cgatcgtcga aggcaagcgc
     4861 gtggattccc agttctgcca tcgagacaga atcggaaatc gtatcttggg acggtggatg
     4921 gggcatgaga gcagatccca acgctgtctg ttcgcttgca ccggcaaaag gaagggtagg
     4981 cttgatgaat cgggtcgatt ccctcatgcc cagaagttca ctggcgccga tcgagccacc
     5041 gaggtcgacc caccattttc gtttctctga ctcgctcgcg cccccatagt catgaaggaa
     5101 cgattcggat gcttgcgcga agacttcgga agcctgccat gacgactcgc tggcacccat
     5161 ccgcagttgc tcgctggcac ccatccgcag ttgctcgctg gccccgataa aaacccgttc
     5221 gctgccctgt tccagaaccc attcggaagc tcccagcccg gcgaattgcc attccgtaac
     5281 acgctcactg gcacccgcca gacgcagttc actagcgcca agccagatcc attccgagcc
     5341 tcccagcgcc aattgctcct cgatggcgta ggaaatccgt tctgaacctc ccagtatcca
     5401 acgttcgctc gcgccttgct ccacgtcttg ccgcatctcg gaagctccag tccacgtacg
     5461 ctcacgaacg aatccttcgt gccaaatacg ctccacgatg ctttcccaac gctccaccac
     5521 ggattcgatc gtttgcgctc gatgcagcct ccaagttcca agaacccggc gaaaagacgt
     5581 tccaaacgag gctccgtgaa tgacgatctc ccaggatcca gcagattcct tttcgaagcc
     5641 actatccata tacgtatagt cgaccccgag gaaacgaacg aggacacgtc cgggagccgc
     5701 gctatctccg ggatagggcg cccaatccca cgtggtgagc acttgttcac gcgaccaggc
     5761 cacgttgtga agcggacgaa tccactcgaa agcccgctca tcgccttgcc actctgtccg
     5821 ccaactttcg gtcatccag
//
LOCUS       DAOSOR010000371         5829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13857_length_5829_cov_4.454797, whole genome shotgun sequence.
ACCESSION   DAOSOR010000371 DAOSOR010000000
VERSION     DAOSOR010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5829
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13857_length_5829_cov_4.454797"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PK710_17420"
     CDS             <1..282
                     /locus_tag="PK710_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11562.1"
                     /translation="DGSLSPDVLQRLTGGSANAAQESSSGGLMGLLGSIPGVAQVKLA
                     LEGVAALKNGDASKVLQLASDLVPVPGVRSALQTASQAVDAVKSAGKTK"
     gene            550..2739
                     /locus_tag="PK710_17425"
     CDS             550..2739
                     /locus_tag="PK710_17425"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOT11563.1"
                     /translation="MPRLSTITRALMMAGLLSACATQVSVTPLPPAPTASSEPAVAEP
                     VAVNPLLEPWTGPYGGVPPFDKVQLSDFRPGLEQSMAMALEAVDRIAKDPQPATFDNT
                     LAALERATRAFDRAVAVYGVWSGSKNTDEFQAIEQEMEPKLAGFVDKITQNAELFRRI
                     EVVYQAKDSLTPEQQRLAWKVYHDFVRAGAKLDDAAKKRLSEINQKLATLFTQFGQNV
                     LADEKDQTLVLESEADLAGLPKELVDSAAMEAEARKMAGKWVISNTRSAVDPFLTYSE
                     RRDLRERAWKMFINRGDNGGKTDNNAIITEILALRAERAKLLGYETHAHWRLEDSMAK
                     TPARVLELLEAVWTPAVARVKEEVRDMQAIANKEKAKITIEPWDYHYYAEKVRKQKYD
                     LDESQIKPYLQLDKLREAMFWVAGELFNLQFAPISGVPVFSPEVTVWKVTNKETGKEV
                     GLWYFDPWARSGKRSGAWMSEYRAQERFDGETLPIVSNNANFLKGKPGEPVLISWDDA
                     STLFHEFGHALHGLSSNVTYPSLAGTEVVRDYVEFPSQLLEHWLSTPEVLQKYALHYQ
                     TGKPIPKELVERIEKASTFKQGFATVEYLASAFIDMKLHLAGAQRIDPDAFERDTLKK
                     LGMPRELVMRHRTPQFMHVFSSDGYSAGYYSYMWADVLTADGYGAFTEAGGPYDKAVA
                     KRLRDNVFSVGNTIDPEQGYRRFRGRDATIDALMKKRGFPVPKGKGK"
     gene            3353..3940
                     /locus_tag="PK710_17430"
     CDS             3353..3940
                     /locus_tag="PK710_17430"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT11564.1"
                     /translation="MMVSARLVSAFTLAVQVHGDHVRKGTGVPYLTHLMAVSSLVAEY
                     GGDEDLMIAALLHDTLEDRPDRITLPQIIARFGWRVGHVVHGCTDSHQLPKLPWEQRK
                     TVFLERLHRESADVKLVAAADKLHNASSLLRDARMLGDSHWARFNASKERQCWYLRSC
                     VEALRDGWSNEIVVTLKQTVENLERLCGITVGSTG"
     gene            complement(3958..5109)
                     /locus_tag="PK710_17435"
     CDS             complement(3958..5109)
                     /locus_tag="PK710_17435"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT11565.1"
                     /translation="MGRTLLLMLYSVMRKEVLQAFRDKRMATLLLIAPLMQLVVLGYA
                     VDLEVDHIPTVVCDQDRTPASRALLEATFAEGTFQRVATVTRPAEAVAQVTDGKAASA
                     WIIPRGYEQALARGTGAAVQVLIDGTDTNRAQVASAAAQQMALFRSVEISTQSLASSS
                     ALAQRQASTAQISPVARVLYNPNLRSATYMVPGLAATVVLIVTTIVTAMGIAREKEMG
                     TMEQLLVTPLRPWILLVGKCLPFAAVGLLDVAGVIAIGAWLFDVPLRGSLLVVFVGAA
                     FYVLSTLGMGVFISTVSSSQQQAILGGFLFLLPAILLSGFMTPIDNMPQPIQWLTYAN
                     PVRYFIEVMRSVLLKGAGFAELGFQLVALASFGVLILALSAFRFKKQLD"
     gene            complement(5126..>5829)
                     /locus_tag="PK710_17440"
     CDS             complement(5126..>5829)
                     /locus_tag="PK710_17440"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT11566.1"
                     /translation="VTQAQTMSLVASKTPQHRLALQAQTRMLFNPGMRSSLFVVPGLI
                     ALILAIMAVLLTALTVAREWERGSMEQLFSTPVRRVEVVVGKLLPYLAVGVIQVLLVT
                     ALGAWLFDVPLRGSVALLFASSILFLTGMLGQGLLISVLTRNQQVATQVGIITSLLPT
                     LLLSGFLSPVENMPLALQAIAMILPSTHFIVILRGILLKGNGAEVLWPHIAALAAFAS
                     VMIGVSTARFRRRLA"
BASE COUNT         1307 a         1694 c         1669 g         1159 t
ORIGIN      
        1 gatggttcct tgagccccga cgttctgcag cggctgaccg gaggctccgc caatgcagcg
       61 caagaaagca gctcgggcgg gttgatgggc cttttaggat ccatccccgg cgttgctcaa
      121 gtcaaacttg ctctcgaagg cgtggccgcc ctcaaaaacg gcgatgcctc gaaagtgctg
      181 caactcgcct cggatctcgt accagtcccc ggtgtccgca gcgcgttaca gaccgcttcc
      241 caagccgtgg atgccgtcaa aagcgctgga aaaaccaagt aacccgccgg cgccgggccc
      301 aaggtcagcc tacctcgcct ccccagccca tcggcatccc tcccaaccgt cttcccgtac
      361 ccctcgtact aggggaatca taataaaata taatcaattc aactagttat gcatgcgccg
      421 cccctgtctt cgctgtctct tgcctggcgc tatcccagcg cgatccgtcg gtgatacgaa
      481 caaacggttg tattcggcgg gcggggaagc tagaccggta gccatcgatc ttgccaggag
      541 atgatgccga tgcctagact gtcgacgatt acgcgagccc tgatgatggc ggggttgtta
      601 tccgcatgtg cgacgcaagt ttccgtgacg cctttgccgc cggcccctac ggcttcttcg
      661 gagccggccg tggctgaacc ggtggcggtc aacccgctgc tcgaaccctg gacggggcct
      721 tatggtggcg tgccgccttt tgataaggtg cagttatcgg atttccgacc gggattggag
      781 caatcgatgg cgatggctct cgaggcggtc gaccgaattg cgaaggatcc gcaacctgcc
      841 acgttcgaca atacgctggc ggcgttggag cgcgcgacac gcgcctttga tcgggcggtg
      901 gcggtctatg gggtttggag tggatcgaaa aacaccgatg agtttcaggc catcgagcag
      961 gaaatggagc cgaaactcgc aggtttcgtg gataaaatca cacaaaacgc cgaattgttc
     1021 cggcgcatcg aagtggtgta tcaagccaag gattcgctga cgcccgagca gcaaaggcta
     1081 gcgtggaagg tctatcacga cttcgttcgc gcgggggcca agctcgatga tgcggcgaaa
     1141 aagcgccttt cggagatcaa ccagaagctt gccacgttgt tcacacaatt tggacagaac
     1201 gttctcgccg acgaaaaaga tcaaacgctc gtattggagt cggaagccga cctggcgggt
     1261 ctgcccaagg aactcgtcga ctcggccgcg atggaagcgg aagctcgaaa aatggcagga
     1321 aagtgggtga tttccaatac ccgctccgcc gtcgacccct tcctcaccta ttcggagcgt
     1381 cgggatttgc gcgaacgcgc ctggaaaatg ttcatcaacc ggggggataa tggcggtaag
     1441 accgataata acgcgatcat caccgagatt ctcgccttgc gggccgagcg agccaagctc
     1501 cttggctatg aaacccacgc ccattggcgt ttggaagact cgatggccaa aacgcccgcc
     1561 cgtgtcctag aactgttgga ggccgtgtgg acacccgccg tggctcgcgt caaagaagaa
     1621 gtccgggata tgcaggccat cgcaaataaa gaaaaggcca agatcacaat cgagccttgg
     1681 gactaccact attacgccga aaaggtgcga aaacagaagt acgacttgga cgaaagccag
     1741 ataaaaccgt atctccagct cgacaagttg cgagaggcca tgttctgggt ggccggcgaa
     1801 ctgttcaatc tgcaattcgc cccgatctcc ggcgtgcccg tgttttctcc cgaagtgacc
     1861 gtatggaagg tgacgaacaa ggaaacggga aaagaggtgg gcctctggta cttcgacccc
     1921 tgggcgcgat cggggaaacg ctccggcgcc tggatgagtg aatatcgggc tcaggagcgc
     1981 ttcgatggcg agacgttgcc catcgtatcg aataatgcca atttcctgaa aggtaagccg
     2041 ggcgaacccg tgctcatttc ctgggatgac gcttcgacgc tatttcatga gttcggtcac
     2101 gcccttcatg gcctatcttc gaatgtgacc tatccttcgt tggcgggaac ggaagtggtg
     2161 cgcgactacg tggaattccc ctctcagctt ctcgaacact ggctttccac gcctgaggtg
     2221 ttgcagaagt atgcgctgca ttaccagacg ggcaaaccca ttcccaaaga actcgtggag
     2281 cgaatcgaaa aggcttcgac gttcaagcag gggtttgcaa cggtagaata cctggccagt
     2341 gcgttcatcg acatgaaatt gcacctggct ggcgcccagc ggatcgaccc ggacgctttc
     2401 gagcgggaca cgctcaagaa gttgggaatg ccgcgggaac tggtcatgcg ccatcgaacg
     2461 cctcaattca tgcatgtttt ctccagcgac ggctattccg ccgggtatta tagctatatg
     2521 tgggccgacg tgctgaccgc cgatggttat ggggcgttta ccgaagcggg ggggccgtac
     2581 gacaaagcgg tggcgaaacg ccttcgagat aacgtctttt ccgtgggcaa taccatcgat
     2641 ccggaacagg gatatcgtcg gttccgcggg cgtgatgcca ccatcgatgc gctgatgaaa
     2701 aaacgcggct tcccggtccc caagggcaag ggaaagtagc ctatagcgct ttttcttatc
     2761 ctggcccccc gatccacggc tgagccaacc cctcattttt cccgagcctt tccttcatct
     2821 tcgactgctg gaaacaagta cctgccatca tcgtcacggc acacgcaaaa cggtcacggt
     2881 cacggtcacg gtcacggcac acgcgaaacg gtcacggtca tcccattcca ttttgttcgc
     2941 cgcaaggcgc acataagtta tggaaattac tcggatttac taattctggt gtcaaagtcg
     3001 gcaactacgc tcaacgtatc gtaagatgcc gacgcatcga aaacatctcg gtttcctgca
     3061 gcctttctac acaggacacg cccgaccatc tgcggaatac cgttccagcc gagccgcgaa
     3121 gcatcctggc tgggctggaa atgacgcgtc tggcgtatag tcaaatccgg taccgtaggg
     3181 gcgggtttca aacccgcccc cctgcctgag cccgaaatac gaaaaagcgc aagcctcctg
     3241 cgcttttccc atcccctgct tgcataacga accccctatt cccggttgat ttcgggccca
     3301 tggatctcaa tcgattcctc cctcggctcg aatgatgcta cactgccgcg ccatgatggt
     3361 ttccgcacgc ttggtttccg cttttactct tgccgtgcaa gtccatggcg atcacgttcg
     3421 aaaaggtacg ggcgtgccct atctcacgca tttgatggcc gtcagctccc tggtggccga
     3481 gtatgggggc gacgaagacc tcatgattgc cgccctcttg cacgatacgc tcgaagacag
     3541 gccggatcgc atcacacttc cgcagatcat agcccgcttc ggctggcgcg tcggccacgt
     3601 cgtccatggc tgcaccgatt cccatcagct acccaaactg ccctgggaac agcgaaaaac
     3661 cgtcttcctc gaacgcctcc accgtgaaag cgcggacgtc aaactcgtcg ctgccgccga
     3721 caaactccac aatgcatcgt cgttgttgcg cgatgcccgc atgttgggtg actcccattg
     3781 ggcgcgcttc aatgcctcca aagaaagaca atgctggtac ttgcgatcgt gcgtggaggc
     3841 gttgcgggac ggttggtcca acgagatcgt ggtcaccctc aagcaaacgg tcgaaaatct
     3901 ggagcgccta tgcgggatca cggtgggatc gaccggctga attcctttgt aaattcatta
     3961 gtctagctgt tttttgaacc gaaaggcgct gagggcaagg atgagcacgc cgaaactcgc
     4021 caacgccacg agctgaaagc ccaattccgc aaatcctgcc cctttgagca gcacgcttcg
     4081 catcacttcg atgaaatagc gaacggggtt ggcgtaggtc agccattgaa tcggttgcgg
     4141 catattgtcg atgggggtca tgaatccgga tagcaagatg gccggcaaca agaacagaaa
     4201 ccctccgagg atcgcttgtt gttgagagga actcacggta ctgatgaaaa cgcccatgcc
     4261 caaggttgat aagacgtaaa acgcggcgcc gacgaaaacc acgagcagcg aacctcgaag
     4321 aggaacgtcg aaaagccagg cgccgatggc gatcacgccc gcgacatcga gcagccccac
     4381 ggccgcgaaa ggaaggcact ttcccacgag caatatccag ggtcgaagcg gggtgaccag
     4441 aagctgttcc atggttccca tctctttttc tcgggcaatt cccatggccg ttacgatggt
     4501 ggtcacgatc agcaccacgg tggcggcgag cccgggaacc atataggtgg cagaacgcag
     4561 gttcgggttg tagagcaccc tggcgacggg ggagatctgc gccgtggagg cttgtcgttg
     4621 cgccagggcc gacgaggacg ccagcgattg tgtgctgatt tccaccgagc gaaagagggc
     4681 catctgctgg gcagcggcgg aagccacttg ggcgcgattc gtatcggttc cgtcgatgag
     4741 cacctgcacg gcggcacccg taccccgcgc caatgcctgt tcatagccac ggggtatgat
     4801 ccaagcgctg gctgccttcc catcggttac ttgggcaacc gcctctgctg gccgtgtcac
     4861 cgtcgccacc cgttgaaacg tgccttccgc gaaggtggcc tccaacaacg cacggcttgc
     4921 gggcgtgcgg tcttggtcgc atacgacggt ggggatgtgg tcgacttcga ggtcgacggc
     4981 gtagcccagc accacgagct gcatgagagg cgcgatgagc aacagtgtgg ccatccgctt
     5041 gtcccgaaag gcttggagaa cctctttgcg catcacggaa tagagcatga gtaggagtgt
     5101 tcgtcccatg gaatcatgtc cttttttatg ccaatcgacg acgaaaacgg gctgtggaca
     5161 cgccaatcat gacgcttgcg aaggccgcca aggcggcgat atgcggccaa agaacctccg
     5221 cgccgttgcc tttcagcaga atacctcgca aaatgacgat gaagtgggtg gacggcagga
     5281 tcatggcaat ggcttgtagc gcaagcggca tgttttccac cggagataga aagccactga
     5341 gcaataacgt gggaagcagc gatgtgataa tgcccacttg ggtggcgacc tgttggttac
     5401 gagtcaatac gcttatcagg agaccttgtc ccagcatccc tgtcaaaaac aagatggaac
     5461 tcgcaaatag gagcgccacc gaccctcgca ggggaacgtc gaagagccag gctcccaaag
     5521 ccgtcacgag aagaacttgg atcaccccca ctgccaagta aggtaggagt ttgcccacga
     5581 ccacctctac tctgcggact ggcgtggaaa agagctgctc catgctgccc cgctcccatt
     5641 cccgcgctac cgtcaacgcc gtgagaagca ccgccatgat ggccaaaatc aacgcgatca
     5701 atccgggaac cacgaaaagc gacgaccgca tccccggatt gaaaagcatc cttgtttgcg
     5761 cctgcaatgc gagtcgatgc tgcggtgttt tcgaggccac cagcgacatc gtctgggctt
     5821 gggtgaccc
//
LOCUS       DAOSOR010000372         5814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13917_length_5814_cov_3.868554, whole genome shotgun sequence.
ACCESSION   DAOSOR010000372 DAOSOR010000000
VERSION     DAOSOR010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5814
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13917_length_5814_cov_3.868554"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PK710_17445"
     CDS             <1..320
                     /locus_tag="PK710_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11567.1"
                     /translation="SFFDSTAPASTLASPGTMVDSLSTDPMHAHSLVWALGSVLSTTA
                     AKDQVRIHRLGPHFELLSPKDQPIVVDDALAHAIVGGTRLPLLFRIHQSTGELDVSPV
                     GCP"
     gene            317..1540
                     /locus_tag="PK710_17450"
     CDS             317..1540
                     /locus_tag="PK710_17450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11568.1"
                     /translation="MKHFGGALCFALALPSFGCGEDMNTSDTESSDGGNALPPIETCN
                     FVDDDGDGLVDEGFPWVVEDPVLLHRVSRFSTLGQVVPAADNTWILVGTDAISAPGDR
                     LFALSIDEKAQVIHGPTHVAIPHCSQGAAVLAPLDSNFLALTGSRTWEGQSPCPEDGC
                     PLLGVIFDPELHVVTKASLTIPNSPYPLVSGLECVFSACFAIVRDGMTVSLLRLAPSP
                     LALEAQREVGQDAVLLSVSADDEQLLDIVDVENARVRWRRLRGQDLTEEGPPMELLAA
                     NRIHGAKALEDGGFILAYADSAEMPHVARFEADLQRRFDRSDVVPPLDMVGRGNGILY
                     MAIPTDNLVLAVELTRLDESFFPVRAPNNPLRVNTYPSKVSLMIGENSALMFSSNYDG
                     QAIEAHRLSCGKQSP"
     gene            1537..2823
                     /locus_tag="PK710_17455"
     CDS             1537..2823
                     /locus_tag="PK710_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11569.1"
                     /translation="MRISSFLPIGFGLAAAFSLGSCHEIAGLEEHVLLPSSDGREPDE
                     DDVWQVPAEQCNFRDDDFNGLTDEGFSWALSDWSPIASGYDWQELRAVRMPDGAVGFG
                     VSRQNGPSSTDLWVGLLDADGSVISAPREVAVEQVLHGWDVAVNPVLAELAIAYVRQP
                     PTEENCEKGCIFEMLRFDRALSPKTEAPEKTNFPRHRVRRLFDLDWNTAGYVSLTLDK
                     DGYAWVEWIDHLHFVFPNGGYGKIPGMRPDLARMSVGPATAWTAMGLTDADRYEVWSG
                     VFSPGGYQRIVEGHPMASSSSALALGKGNSVAWGPNGAMVLMAEGGGDSAGTLLLGAV
                     ATGETRVAGQASNPVAHSLTAVGSSLIASSSAGASTTLLWRWEDPPGDGSSTPFATLE
                     GTLDHVVVAGGYPPLVLRVTADGKNLQVARVHCVEP"
     gene            complement(3157..4134)
                     /locus_tag="PK710_17460"
     CDS             complement(3157..4134)
                     /locus_tag="PK710_17460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11570.1"
                     /translation="MNHPRAITFELITSTALFSHGSNPKGDAEVRAPPFEALARYFFR
                     ALVGDRLSAPCGGATATCTCLRCEEGRVFGSADHGKSVALVVEGNASNRGTCNLLPHQ
                     NSSPSSAVVPEQELFLQLMPALPTTRDENLKAAGATLLVGLLLGGIGQRSRRGAGSLR
                     IKSLKGPIGIDISAFQQASSVKDYAKALETVLRDAKNMALASVCPLPHQAAGGTFPML
                     TKKSAGIMLMEFDAKSESDARAEVMFKLRRHKNAAFGLPYLKPAQGDNEKRGRHASPL
                     WIRLAPFHKRWLATLTVMKSGTLAGNPGKLDKYLDDIHTRHGGISVAIP"
     gene            complement(4131..5510)
                     /gene="cmr6"
                     /locus_tag="PK710_17465"
     CDS             complement(4131..5510)
                     /gene="cmr6"
                     /locus_tag="PK710_17465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr6"
                     /protein_id="HOT11571.1"
                     /translation="MVLGAPDYVGKEFGDAPPGHRFRLYFDGWNFNAQGKFEAKKDRA
                     ALERYSNYPRLSLMLVKELRDRQLMHAHPSDFVCHLKATSSFVTGMGLDHPIENGFAF
                     LDPYGLPYLPGSSIKGVLRRAAEELALFTTIGAEPWELGDIWWLFGIDGQSAFWRAAP
                     KGETPEAKLARETWQKKFEEAVERWSQQNRLVELDPLFEALGEDWKKNRKKTLLELHK
                     RPSALGKLHNKGALECWDAVLLPPNNTMRVDVMTPHFSGYFQDKKKPKAPTDDQDPTP
                     IPFMAMPKGTKMRVIMRYRPTHSHPPALGGRWIKLIDSAMLAACGWVGFGAKTTAGYG
                     RWAIDESEQKHWIDRARQQEKERQQKQQEEAERKVKEAKEQRLAQLSPEERILEELRE
                     ATKAGDKDVVMAIYPQFEAARGAEKLALAEGLREAFKALGKWEGKQSKKQEQKKKAIL
                     EALKEGGKA"
     gene            complement(5513..>5814)
                     /gene="cmr5"
                     /locus_tag="PK710_17470"
     CDS             complement(5513..>5814)
                     /gene="cmr5"
                     /locus_tag="PK710_17470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III-B CRISPR module-associated protein
                     Cmr5"
                     /protein_id="HOT11572.1"
                     /translation="PSMIMSNGLMQTLAFYQAKGKPIRADITTWLGHRMGKELPAGNG
                     KGADQNVAMGKGEDFRIVMSFLQNASSEQYMRATEEALTVLKWIRHFAAALKKGG"
BASE COUNT         1166 a         1673 c         1648 g         1327 t
ORIGIN      
        1 tttctttctt tgactcgacc gcgcccgcaa gcacgttggc ttcccccgga acgatggtag
       61 acagcctttc aaccgatccc atgcacgccc attccttggt gtgggccttg ggcagcgtct
      121 tgtctaccac cgccgccaag gaccaagtgc ggatccaccg cctgggccct cacttcgaac
      181 tgctttctcc caaggatcag ccgatcgtcg tggacgatgc cttggcccat gcgatcgtcg
      241 gcggtactcg gcttcccctt ctgtttcgta ttcaccaaag caccggggag ctggacgtct
      301 cccctgtagg atgcccatga agcacttcgg tggcgccctc tgtttcgcct tggctctccc
      361 atcgttcggc tgcggcgagg acatgaacac gtcggacacg gaatcctccg atggcggaaa
      421 cgcgttgcca cccatcgaaa cgtgcaactt cgtcgacgac gatggcgatg ggctggtcga
      481 tgagggattc ccttgggttg tcgaggatcc cgtgctcctt caccgggtca gcagattctc
      541 caccctcgga caagtggttc ccgcggcgga taatacatgg atcttggtgg gaaccgatgc
      601 cattagcgcg ccaggagacc gtctcttcgc gctttcgatc gacgaaaaag cgcaggtaat
      661 tcatggacct acacatgtcg cgattcccca ttgcagccag ggggcggccg tgttggcgcc
      721 gttggactcc aactttctcg cactaacggg gtctcggacg tgggaaggac agtcaccgtg
      781 cccggaagac ggctgtccac tgctgggcgt gatctttgat cccgaactcc acgtcgtcac
      841 aaaggcttcc ttgacgatac cgaacagtcc ctatccgcta gttagcgggt tggaatgcgt
      901 gttttccgcc tgtttcgcca tcgtgcgcga cgggatgact gtgtcgctcc ttcgattggc
      961 gccttctcct ctggcccttg aggcgcaacg ggaggtgggg caggatgccg tgttgctgtc
     1021 cgtatctgcg gatgatgaac aactgctgga tatcgtcgac gtggaaaatg ctcgcgtgcg
     1081 ttggagaagg ctgcgaggcc aagatctgac cgaagaaggt ccaccgatgg aacttctggc
     1141 agccaaccgg attcatggtg ccaaagctct cgaggacgga gggttcatcc tcgcgtatgc
     1201 ggactccgcg gagatgcctc acgtggcgcg attcgaagcc gacttacaac gtaggttcga
     1261 caggagcgat gttgtgccac cgctcgacat ggtgggcagg ggaaacggta tcctatacat
     1321 ggcaataccc acggataatc tggtacttgc agtagagctg acgcgtctgg acgaatcgtt
     1381 tttccccgtg cgcgcgccga ataacccttt gcgtgtgaac acctatccct cgaaggtatc
     1441 gctcatgatc ggtgaaaact cggcgttgat gttcagttcg aactacgacg ggcaagcgat
     1501 cgaggcccat cgtctttcct gtggcaagca atccccatga gaatcagttc cttcttgcca
     1561 attgggttcg ggctcgcggc agcgttttcc ctcggaagtt gtcatgaaat cgcgggtttg
     1621 gaggaacatg tcctgctgcc ctccagcgat gggcgagagc cggacgagga tgacgtctgg
     1681 caggttcccg ccgagcaatg caacttccgc gatgacgatt tcaatgggct gacggacgaa
     1741 ggattttcgt gggcattgag cgattggagc ccgatagctt ccggttacga ttggcaagaa
     1801 ctacgagccg tcaggatgcc ggatggagcc gttggcttcg gggtgtcgag gcaaaatggc
     1861 ccttccagca ccgatctatg ggtggggctc ctcgatgcag atggctcggt gatctcagct
     1921 ccgcgcgaag tcgccgtgga gcaggtgctc catggttggg acgtcgcagt caatcccgtc
     1981 ctggcggagc ttgcgatcgc ctacgtgcga cagcctccca cggaagagaa ctgcgaaaaa
     2041 ggttgtattt tcgagatgtt gcgcttcgat cgcgcgcttt cccccaaaac ggaagcaccc
     2101 gaaaaaacca actttccgcg ccatcgagtg cggcgattgt tcgacctgga ctggaacaca
     2161 gcggggtacg tatcgcttac gttggacaag gacggatacg catgggtgga gtggatagac
     2221 cacctgcatt tcgtctttcc caacggcggt tacggcaaaa taccgggaat gcgcccggat
     2281 ttggcccgta tgagcgtggg gccagcgacg gcgtggacag cgatgggcct taccgacgct
     2341 gaccgttacg aagtttggtc tggagtgttc tcccctggag gataccaaag aatcgtcgag
     2401 gggcatccga tggcctcttc gtcatcggct ctcgcccttg gcaaaggaaa ttcggtggct
     2461 tggggaccca acggtgcgat ggtgctcatg gccgaaggtg gaggtgattc tgccgggaca
     2521 ctgctgcttg gcgcggttgc tacgggtgag acccgcgttg cgggccaggc ctcgaaccct
     2581 gttgcacact cgttgaccgc ggtgggttca agtctgattg cttcctcctc ggcgggtgca
     2641 tcgacgacgt tgttgtggcg atgggaagac cctccgggcg acggttccag cacgcctttc
     2701 gccacgttgg agggaacttt ggaccacgtt gtggtggcgg ggggatatcc gcccttggtc
     2761 cttcgggtga cggcggatgg gaagaatctg caagttgcgc gagtacattg tgtcgagccg
     2821 tgaattcgaa gagcgccggg gcaggggagg ggacgatggc aagggcagac gcggctccga
     2881 ggggcatggg gcgtattgat gtaactcatg gtatttattc agtttcagga ttgacgagcg
     2941 caggcgctgg gatggaaact gggtccggcg aaacaagata ttcgaacaag caagaaggaa
     3001 gcctctcgca cctagggcga atcatcattc gcccgtgaat cgtcggtgaa tggcgagaaa
     3061 tgccttgaag gcatagaggc aaaggggtta cgaagaaaat gctcaggggc aaccatgaga
     3121 ggacggattg gccttttacg cccaacgact cctgattcag ggtatggcaa cggaaatacc
     3181 gccgtggcgg gtgtgaatgt catcgagata tttgtcgagt ttgcctggat ttccagcgag
     3241 tgtcccactt ttcatgaccg tcaaggtagc cagccatcgc ttatgaaaag gagccaggcg
     3301 tatccacagc ggagaagcat gacgaccgcg tttctcattg tctccctggg cgggtttcag
     3361 ataaggcaac ccaaaggcgg cgtttttgtg gcgccgaagc ttaaacatca cctctgctcg
     3421 cgcatcggat tcacttttgg catcgaactc catcagcatg ataccagcgg attttttcgt
     3481 cagcatgggg aaagtacctc ccgcagcttg gtggggcagg ggacataccg acgccaatgc
     3541 catgtttttc gcgtcccgca gcaccgtttc cagggccttg gcataatctt tcacggagct
     3601 ggcttgttgg aacgccgaaa tatcgatgcc aatcgggcct ttcaagcttt ttatcctcaa
     3661 actacccgcg ccccgtcgcg aacgctgccc gatacctccg agaagcagtc ccaccaaaag
     3721 ggttgccccc gccgctttta ggttttcatc ccgcgtggtt ggcagcgctg gcatcaactg
     3781 caagaataac tcctgctccg gcaccacggc ggaacttggg gaactgttct ggtgaggaag
     3841 caagttgcaa gtaccgcggt ttgaggcgtt tccctccaca accaaagcca cacttttccc
     3901 atgatccgcc gacccaaaca cacgaccctc ctcacaacga aggcaggtac acgttgccgt
     3961 tgccccacca cagggcgcgg aaagacggtc gcccacgaga gcacggaaaa agtaccgcgc
     4021 aagggcctcg aaaggcggtg ctcgcacctc ggcatcccct ttgggattcg aaccatggga
     4081 gaatagcgcg gtcgaggtta tcaactcgaa cgtgatggcg cgagggtgat tcatgccttg
     4141 cctccctcct tcaacgcctc gagaatcgct ttcttcttct gttcctgctt tttactctgt
     4201 ttcccttccc acttgcccaa cgctttgaat gcttctcgga gcccctcggc caacgcgagt
     4261 ttttctgcac ctcgggccgc ctcgaattgc ggatagatgg ccatcacaac gtctttatcc
     4321 cctgctttcg tggcttccct cagttcttcc agaatccgct cctcagggga aagctgagca
     4381 agacgttgct ccttcgcctc tttgaccttt cgttccgctt cttcctgctg cttttgctgt
     4441 ctttcttttt cttgctgccg ggctcgatct atccaatgct tttgctctga ttcatcaatg
     4501 gcccatctgc catagccagc cgtcgttttt gcaccgaaac ccacccaccc acacgctgct
     4561 aacatagcgg aatcaatgag ttttatccat cgtcccccga gagccggagg gtgggaatgc
     4621 gtgggacgat accgcatgat gacccgcatc ttcgtgccct tcggcatcgc catgaacggg
     4681 atcggggtgg gatcctggtc gtccgtggga gccttgggct tctttttgtc ctggaaataa
     4741 ccggagaaat gaggcgtcat cacgtcgacc ctcatggtgt tgttcggagg aagcaaaacc
     4801 gcatcccaac attccagcgc tcccttgttg tgcagcttgc ccaacgcgga cgggcgcttg
     4861 tgcaattcca acagagtctt tttgcgattc tttttccaat cttccccaag agcctcgaaa
     4921 agagggtcca gttcaacaag ccggttctgc tggctccagc gctcgacggc ctcttcaaac
     4981 tttttttgcc atgtctcgcg agccagcttg gcttctggcg tttcgccctt cggagcagca
     5041 cgccagaacg cgctctgccc atcgatacca aataaccacc aaatatcgcc gagttcccaa
     5101 ggttctgcgc caatcgtcgt aaacaacgcc aactcctccg ctgcccgacg caatactccc
     5161 ttgatgctgc tgcccggcag ataaggcaga ccgtagggat cgagaaaggc gaagccgttt
     5221 tcaattggat gatccagccc catgcccgtg acgaacgatg acgttgcttt gagatggcaa
     5281 acgaaatccg atggatgcgc atgcatgagc tgccggtcgc gaagctcctt caccaacatc
     5341 aggctcaatc gggggtagtt cgaataacgt tccagcgccg cacgatcctt ttttgcctcg
     5401 aacttccctt gagcgttgaa gttccaccca tcgaaataaa gccgaaaacg atgcccgggt
     5461 ggagcatcgc caaattcttt ccccacatag tccggtgccc caagaaccat cgtcagcccc
     5521 ctttcttcag agccgcggca aagtggcgaa tccacttgag tacggtcaaa gcttcttccg
     5581 ttgctcgcat atattgctcg gaactcgcgt tttggaggaa gctcatcacg attcggaaat
     5641 cctcgccttt gcccatggca acgttttggt cagctccctt gccgttgcct gctggtagtt
     5701 cctttcccat tctatggccg agccacgtgg taatatccgc tcgaatgggc tttcctttgg
     5761 cttgatagaa cgccagggtc tgcatcaacc cattcgacat gatcatcgaa ggcg
//
LOCUS       DAOSOR010000373         5813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13921_length_5813_cov_4.138242, whole genome shotgun sequence.
ACCESSION   DAOSOR010000373 DAOSOR010000000
VERSION     DAOSOR010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13921_length_5813_cov_4.138242"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..1109
                     /locus_tag="PK710_17475"
     CDS             216..1109
                     /locus_tag="PK710_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11573.1"
                     /translation="MQPRFRIIVGFALSFLVMLAACSSDPKSETESPVDAGSESALID
                     HAVVEHDDGFEASEEAVPDAPNEQPPIPTCPPDSWGNVPNTQCQLIEQSCESKLLTCF
                     PSVINGIAGTSCTALGYGAKTRGAVCESHSECASGLACLAGNCTPFCCKEHQHEICGP
                     GGQCSISLTLGPKYWVYVCGYSEPCTLWLNDCPEQEACHALITDGTASCSPPSSGSFV
                     AEGASCSARNDCGDSQACVSHEGSGNRCRYLCKRGDSPWDAGAPNAQPGKGGCPDGQS
                     CQKLNEAPDWLGVCVPTKPNG"
     gene            complement(1099..2511)
                     /gene="lpdA"
                     /locus_tag="PK710_17480"
     CDS             complement(1099..2511)
                     /gene="lpdA"
                     /locus_tag="PK710_17480"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOT11574.1"
                     /translation="MTKTTFDAVVIGGGPGGYVCAIRLAQLGLKVACIEKENVGGVCL
                     NWGCIPSKALISVAHTYERAIHGSTMGLLVQGVEVDAARMQSWKNGVVDKLTGGIRQL
                     FKANGVTLIEGKARVTSARSVEITRNDGTLMQVDCSRAIVVATGATTIEIGSLPMDGK
                     QVIGAKEAVSLPAIPKRLCVVGGGVIGLELGTVYHKLGAKLTVVEALPQLLTGIEPDC
                     TQVVERKLKKSGAKILKNAKALGYERQADGSLRLQVQVGDNVESVDCDVLLVAVGMRP
                     NGKGLGLEQLGVIVQQNGAVTVDEKGATPVEGVYAIGDVTGPPFLAHRASKQGEVVAE
                     VIAGQASSCDWVTIPAAVFTDPEIASAGMTTEQATAAGYQIKTGKFPFAALGRALAMG
                     ESDGFIKTLFDASSERLLGVHIVGPSASDLIAEAALALEVGTVAEDIAMTVHAHPTLA
                     EGLMESAAHALGHAIHAVNR"
     gene            2638..3372
                     /locus_tag="PK710_17485"
     CDS             2638..3372
                     /locus_tag="PK710_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HOT11575.1"
                     /translation="MGLFDRFSKVISSNMNALLDKAEDPQKSVDYLIEEMREQIRKGR
                     QEVVQAVAAEKRLRAKVDEIDEQIDKWQRRAELAVQAGDENLAKEALLFKKKLVGERD
                     RAEALRAEQRSQALQLKDDLERADAKLKELDAKKGTIVARYQQARAGGGAEALGARGD
                     GPTPFDELRRMEEKMDRAQTEAEAMKEARDALERPADGRMTQGELEARFEQLEKGDSV
                     DKSSSSGDDDVDDELRALRNKYRITS"
     gene            3386..>5813
                     /locus_tag="PK710_17490"
     CDS             3386..>5813
                     /locus_tag="PK710_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11576.1"
                     /translation="MAERIVVCLSEPALSENGIDFVTHVRGLMLRAKAFGATLCAWSA
                     DAVAFSFQTDDLEEAVGFVTGPDAEPLARAFRIAIVQGSLDILTSDAQIEPGWGPVLH
                     QASELALLAKHGEVLIEKDTLHAHRIRLVTHGSRSSSPSLGLRAYVIDVRDPFHESDD
                     GRADRLFENVWVGRENALSALQIAPGTLATVRAAAGYGASRLLMALAQQQRPAPFLLI
                     SPSGHRMEPLGALRRGLLRARALGSAPSLSESCQSTYLRITQAQGASLAACSELVEQW
                     LTHASGQGLLLIDDVHDVDSPTLEVVTTALLGAEVPFRAVVRMGLDLALPAELAPLPP
                     GPEVDLGPLTKQEAVKLVNEWTGNLLSVRDASHWASQGGGNPLALREIVAEALACGAL
                     IVGKDDAQPRTVPPPAVANAGPDAIISKRFQFVTPGSRAVLFALGVLGGEASLPQIDR
                     ILELCADIPVDLDLEVQRLSEYGWLIAPQPDWIACRSRTHIRVLLHALSETNQFAWYR
                     AACTVVESNGGLLLAEAAWYAAEAHDRVRAHRLAMQAATIAKFAGLESACHDLEAFAR
                     THDPTPRTRLDSDPYDLLARLPMAYSPGTPEEVFPAALADRGPKRPSRFTFPPVAAID
                     TDPVSAPPRPPLASDGQVLGPEDIFPFPPEPDKLDEPPVSALPSSHDEPTGDVSEITI
                     ADRGAFESSTELDPSDHEPTIPERSFLGFSSAHVGLSRERIEALAESIQGSARLVQRI
                     QAIAMLHRGQQTEAVRILRNACEQARELPTLERSRSHLAFAIGLFEAQRPHDALIEAI
                     EALARAREANH"
BASE COUNT         1207 a         1753 c         1657 g         1196 t
ORIGIN      
        1 gcgcggcagg ggctggcgga caagccggcg cggcaggggc tggcggacaa gccggcgcgg
       61 caggggctgg cggacaagcc ggtgcagcag gtgggggagg aatgagtgga gccgcgggaa
      121 ctggcggcac aagcggacaa gctggtgcca gtggaactgg cggcacctga cgttctcatc
      181 caaggagcct ttcgtcacaa cctacggcta ccatcatgca accacgcttt cgtatcatcg
      241 ttgggttcgc gttgtccttc ctggtcatgc tcgcggcttg ctcttcggat ccgaagtcgg
      301 aaacagaaag cccggtggat gcaggctctg aatcagcgct catagatcat gcggtcgtcg
      361 agcatgacga tgggttcgaa gcatcggaag aagcggtgcc ggatgcaccg aacgagcagc
      421 ctcccattcc cacgtgcccg cccgattcct ggggaaatgt tcccaatacg caatgtcaac
      481 tcatcgaaca aagctgcgag agcaagctgc ttacctgttt tcccagcgtg atcaatggga
      541 ttgcggggac gagttgcacg gccttgggct acggtgccaa gacccgggga gccgtttgtg
      601 aatcccatag tgaatgtgcc agtggtctcg catgccttgc gggcaactgc acaccgtttt
      661 gctgcaagga acatcagcac gagatctgtg gccctggtgg acaatgcagt atttcgttga
      721 ccttggggcc caagtattgg gtttacgttt gcgggtattc cgaaccttgt acgctttggc
      781 tcaatgattg cccggagcaa gaagcgtgtc atgcgttgat taccgacggc acggcttcct
      841 gctctcctcc gtcttccggc tcgttcgtgg ccgaaggcgc ctcgtgcagc gcgcgcaacg
      901 attgtgggga ttcccaagca tgcgtatccc acgagggctc tggcaaccgt tgtcggtatc
      961 tgtgtaagcg aggggattcc ccctgggatg caggggctcc caacgcgcag ccaggcaaag
     1021 gaggttgtcc cgacgggcag tcttgtcaga aactcaatga ggcgccggat tggttaggag
     1081 tttgcgtccc taccaaacct aacggttgac ggcatggatg gcatgtccga gcgcgtgagc
     1141 cgcggattcc atcaaccctt cggcgagggt aggatgcgcg tgaactgtca tcgcgatatc
     1201 ttcggccacc gttccgactt ccagagccaa cgcggcttcc gcgatgagat cactcgccga
     1261 cggccccacg atatgcaccc ccaaaagacg ctccgaggag gcatcgaata gagttttgat
     1321 gaatccgtcg gattcgccca tggcaagcgc gcggcccaat gcagcgaagg ggaattttcc
     1381 cgttttgatc tgataacctg ccgcggtggc ttgctcggtc gtcatgcctg ccgaagcgat
     1441 ttccggatcc gtgaatacgg ccgcgggaat ggtgacccaa tcgcacgacg atgcctgtcc
     1501 ggcaatgact tccgcaacga cttcaccttg tttggaagct cgatgggcaa ggaagggagg
     1561 accggttaca tcgccgatgg catagactcc ttccacgggg gttgcccctt tttcgtcgac
     1621 ggtcacagcg ccgttttgtt gaacaattac gccaagttgc tccagtccta gcccttttcc
     1681 attcgggcgc atccccacgg ctacgagcag cacatcgcaa tcgacagact cgacgttatc
     1741 gcccacttgc acttgcagac gaaggctacc atcggcttgt cgctcgtagc ctaaagcttt
     1801 ggcatttttg aggattttgg ctcccgattt tttgagtttg cgttctacca cttgggtgca
     1861 atccggctcg attccggtga gcaattgggg aagagcctcc accaccgtga gctttgcgcc
     1921 aagcttgtga taaaccgtgc cgagttccaa gccgatcacg ccgcccccta cgacgcagag
     1981 gcgtttggga atggctggca agctgacggc ctctttggct ccaatgactt gtttgccatc
     2041 catcggcaac gagccgatct cgatggtcgt ggctccggtt gccaccacga tggctcgaga
     2101 gcagtcgact tgcatgaggg tgccgtcgtt tcgagtgatc tccacggaac gggcagaggt
     2161 cacgcgggcc ttgccttcga tcaaggtcac accattggct ttgaaaagct gacgaatccc
     2221 gcccgtgagc ttgtcgacca cgccgttttt ccagctttgc atgcgcgcag catccacctc
     2281 gactccttgg accagcaggc ccatggtcga cccatgaatc gcccgttcat acgtatgcgc
     2341 cacggaaatc aatgccttcg aaggaatgca cccccaattc aggcatacgc ctcccacatt
     2401 ttctttttcg atgcaggcca ctttcaagcc aagctgagca aggcgaatcg cacaaacata
     2461 gccaccgggc ccgccaccga tcaccaccgc atcgaaggtt gtttttgtca tgatggctac
     2521 ctgtatacac atgctctggt ctgatcggca ccccccatgc ccgggtcttg gccgagaaac
     2581 gtcctagatg ctttacacgc aagcaggatc caggcacgtt atgtaagagg agttcacatg
     2641 ggactattcg accggttcag caaagtcatc tcgagcaaca tgaacgcgct gctcgataag
     2701 gccgaagacc cccaaaagtc cgtggattac ctcatcgaag agatgcgcga gcagatacgc
     2761 aaaggacgcc aggaagtcgt ccaagcggtg gcggcggaaa agcggctccg agccaaggtc
     2821 gacgagatcg atgagcagat cgacaagtgg cagcgacgcg cggaactggc ggtgcaagcg
     2881 ggcgatgaga acctggcgaa ggaggcgtta ttattcaaga aaaagctggt gggggagcgc
     2941 gatcgggcgg aagcgcttcg tgcggaacag cggtcacaag cgcttcaact caaagacgac
     3001 ctggagcgag ctgacgcgaa actcaaagaa ttggatgcca aaaaaggcac cattgtcgcc
     3061 cggtaccaac aagctcgggc tgggggaggc gccgaagccc ttggcgctcg tggcgatggt
     3121 cccacgcctt tcgacgaatt gcgacgcatg gaagaaaaaa tggatcgcgc ccagaccgag
     3181 gccgaagcca tgaaagaagc tcgggatgcc ctggagcgac ccgccgatgg cagaatgacg
     3241 caaggcgaac tggaagcacg attcgaacaa ctcgaaaaag gagactctgt cgataaaagc
     3301 tcatcttcgg gcgatgatga cgtagacgat gaactgcgcg cgcttcgaaa taaatatcgc
     3361 atcacttctt gaatgaatcc gatccatggc tgagcgtatc gtcgtttgcc tgagcgagcc
     3421 tgccctgtca gaaaacggta tcgatttcgt gacgcatgtt cgtgggctca tgctccgcgc
     3481 caaggcgttt ggagccaccc tctgcgcctg gtctgccgat gccgtcgcct tcagctttca
     3541 aaccgatgat ctcgaagaag ccgtggggtt cgtcacagga cccgatgccg aaccgctagc
     3601 acgcgccttt cgtatcgcca tcgttcaagg ctctctcgat attctcacga gcgatgcaca
     3661 gatcgaaccg ggctggggcc ctgtgctgca ccaagcctcc gaactggctc tcctcgcaaa
     3721 acacggggaa gtcctcatcg aaaaagatac cctccacgcc catcgtattc gcctcgtcac
     3781 ccacggctcg cgaagttctt cgccttcact tgggctacga gcctacgtca tcgatgtgcg
     3841 cgatcctttc catgaatcag acgatggacg cgccgatcga ctcttcgaaa acgtgtgggt
     3901 cggacgagaa aacgccttgt ctgcgttgca gattgcccca ggcacgttgg ccacggttcg
     3961 agcggcggcc ggctatggcg ctagccgtct tttgatggct ctcgcacaac agcaacgacc
     4021 tgctcccttc cttttgatca gcccatcagg gcatcgtatg gaacccctcg gtgcgttgcg
     4081 acgaggacta ttacgtgctc gagcgcttgg cagcgcccca tcgctttccg aatcatgcca
     4141 gtcgacctat ctgcgtatca cgcaagccca gggggcaagc ctggcggctt gctcggagct
     4201 tgtggaacaa tggcttacgc atgccagcgg gcaaggactc ctgctcatcg atgatgtgca
     4261 tgacgtggac agcccgacgt tagaggtggt gaccaccgct ttgctcgggg ccgaagttcc
     4321 ctttcgggca gtggtacgca tggggctgga ccttgctctt cctgccgagt tggctccttt
     4381 gccccccgga cccgaagtcg atcttggacc attgaccaag caggaagccg tcaagctggt
     4441 gaacgaatgg acgggcaatt tgttgtcggt tcgcgatgct tcgcattggg cttctcaagg
     4501 aggtggcaac cctcttgctt tgcgcgagat cgtggcggaa gccctcgctt gcggcgcgtt
     4561 gatcgtggga aaggacgatg cccaaccccg aacggtacct cctccggcgg tagccaacgc
     4621 ggggccggat gcaataattt ccaaacgatt tcaattcgtt acacctggtt cgcgcgctgt
     4681 cctcttcgcc cttggcgtgc ttggcggtga ggcttccctc ccgcagatcg accgcatcct
     4741 cgaattatgc gccgacattc cggtagacct cgatctcgaa gttcaacggc tatccgaata
     4801 tggatggctg atcgctcctc aacccgattg gatcgcctgt cggtctcgca cgcatattcg
     4861 tgtccttctt cacgccctgt ctgaaaccaa ccaattcgcc tggtaccgcg ccgcatgcac
     4921 cgtcgtggag tccaatggcg gactgctgct cgcggaagcc gcttggtatg cagcagaagc
     4981 tcacgatcgc gtcagagccc atcgcctcgc catgcaagcg gcaaccatcg cgaaattcgc
     5041 cgggctcgaa agcgcttgtc acgatctcga agcgttcgcg cgaacccacg accccacacc
     5101 acgcacacgc ttggattcag acccgtacga cctccttgct cgtcttccga tggcctactc
     5161 gcctggaacc cccgaagagg tcttccccgc agcccttgcc gaccgcggac ccaagcgccc
     5221 aagccgcttc accttccctc ccgtcgctgc cattgataca gatccggttt cggccccccc
     5281 acggccgcct ttggcctccg atggacaagt cctcggtccc gaggatattt tcccattccc
     5341 gcccgaacca gacaagctcg atgagccccc agtctccgca ctgccctcat cccacgacga
     5401 accgacaggc gacgtttcag aaatcacaat cgcggatcga ggcgccttcg agtcgtcgac
     5461 ggaactcgat ccatcggatc atgaacccac gataccagag cgttccttct tgggattttc
     5521 ttcggctcat gttgggttgt cccgtgaacg gatcgaagcg ttggctgaat cgatccaagg
     5581 aagcgctcgc ctcgtgcaac ggattcaggc tatcgcgatg ctccatcgag gtcagcaaac
     5641 cgaggccgtc cgcattttgc gaaacgcttg cgaacaagcg cgtgaactgc caaccctgga
     5701 gcggtctcga agccatctcg catttgcgat cggattgttc gaagcccaac gacctcatga
     5761 tgcattgatt gaagccatcg aagcgctggc gcgcgcgaga gaagccaacc atc
//
LOCUS       DAOSOR010000374         5808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13937_length_5808_cov_4.035112, whole genome shotgun sequence.
ACCESSION   DAOSOR010000374 DAOSOR010000000
VERSION     DAOSOR010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13937_length_5808_cov_4.035112"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..1625
                     /locus_tag="PK710_17495"
     CDS             273..1625
                     /locus_tag="PK710_17495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11577.1"
                     /translation="MRIPFGFRIGLAGAIPIALIALSFVSCSANSESNTFNQGGGGGG
                     NEEGGTATDGSAANSGGPLFEASTGDGQLDEESACVGQSFPGKLAPLDVYILLDATGS
                     MQGSNNSPVVWPSVVAALKDIISDPLSSGIGVGLTYLPNQPPPTTVIPGSCKPGGDCG
                     ALGLCEFIGIAFACTSACTKNEDCGLYGPCMPWMTPKTPNARVCNGALVPKVSCDPLD
                     YGQPVVPIAPLPGNKDALVQAINDKDPDGDATPTQPSLEGTLIYAKQYAKDNPTHLVH
                     VLFATDGEPNNCTFNTIDGAAVAAATAFSNPPSVPTFVLGIGDLKDLNAIAKAGGTGD
                     AYLANGSTVASQLVTVFNEIRASGACQFQIPEPESGQTLDFDRVNVYYTPLASSEKVP
                     VKYVNDAASCDPKEGGWYYDDPSKTNPTKILLCPATCEGVKLSEEGVEVLLGCKTILK
                     "
     gene            1668..2648
                     /locus_tag="PK710_17500"
     CDS             1668..2648
                     /locus_tag="PK710_17500"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT11578.1"
                     /translation="MHTSVQAIVFFLVAFLVVMGWHAYLWNRLLRDTQPPPRWKVAGK
                     IALLTLVTLFLFAMIGGRSWPFDSLRPLYAIGYGWLGTSFLLVVMLLLTDLGKLSARG
                     VRRLMKRAPTNPERRLTLARILAGSVAFTGFSSTAKGAASALGTIPIRRIRVSLPRLS
                     SSMNGTRIVQISDLHIGPILKRSWVESVVAQVMSLEPDLIVITGDLVDGTVENLRDDV
                     APLAKLKAKAGVFFVTGNHEYYSGAPAWVEHVQQLGIRVLQNENVPIGKGDDAFYLAG
                     VNDFESARHKVGHAHDVAASVRGIPPNREILLLAHQPRSVWDAAKHGVGL"
     gene            complement(2963..3889)
                     /locus_tag="PK710_17505"
     CDS             complement(2963..3889)
                     /locus_tag="PK710_17505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOT11579.1"
                     /translation="MESLLDPNVVQAILDNLSEAVCAFDLQRRITYWNHGAEELTGFA
                     AQEVLGKKCSAGILIHVDDRGRPTCQSGCMLGQALRDRRTNDADLFIRHKNGHRVPVH
                     VRFAPLLNQQQDVIGALQVFREGTASLALAQRVRELERLSFLDPLTNLANRRYVDITL
                     RSMCSQLKHGGIPFGVVLLDIDDLKTINDHHGHEAGDMVLKAVAKTLLETSRPFDVKG
                     RWGGDEYIAIVSNVNSVGLAQTAERFRALIEATAVPYHSIELRTTVSVGATMAQPSDD
                     EFSIVRRADEELYSSKTSGKNRASITGVRMVG"
     gene            complement(4044..5030)
                     /locus_tag="PK710_17510"
     CDS             complement(4044..5030)
                     /locus_tag="PK710_17510"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shewanella-like protein phosphatase"
                     /protein_id="HOT11580.1"
                     /translation="MALWFRYVFFGLVPLVAGCERTPSEPKYVSSAAAPAVSTEADVT
                     DTDRSYRFVAAGRIVAIGDLHGDLAATRKALRVAGVMSDKDSWSGGNTVLVQTGDVLD
                     RGDDERAILDLLDRLEQEAKAAGGLVLRLQGNHEVMNVAGDLRYVTRAGFTTFESVTS
                     PDNPAFAAPFKPQERARALAFAPGGPYALRLARHNTVVMVNDTLFVHGGILSKHIRYG
                     LGRINEEVKAWMRAERPRLPALMLSEDAPTWNRRYSTAHVDARDCRVLATALKHLEAK
                     RMVVGHTPQKGGITSACDDRVWRIDVGLASYYGGGAAQALVIQGDEARVVSE"
     gene            complement(5545..>5808)
                     /locus_tag="PK710_17515"
     CDS             complement(5545..>5808)
                     /locus_tag="PK710_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11581.1"
                     /translation="APAVADTAALAGLAAPAVADTAALAAPAEADTAALVAPRRSIVP
                     HLVRVRALVAAPPQNALRAKHLFAVPFEARRSQHHRLASSQIA"
BASE COUNT         1210 a         1842 c         1486 g         1270 t
ORIGIN      
        1 ggccaataag cgcatcgaaa agtccatgac tggagcataa atggccgaga gcctttgcgc
       61 tagtgttgcc gcgtgattga tcctgccggc ccgctttgcg ggcttcgtcc aaacaagatc
      121 accgatcccc gtttcccgct ttgtgttcgt ctttcgaacc cacgcacaac cgctttcgat
      181 ctgtccgggt tgcccatccc ttctcaacca cggtacacta gatcgattgt cctggcatgc
      241 cgattggctc agcgccggga cgcggaggtc cgatgcgcat ccccttcggt tttcgaattg
      301 gccttgcggg agcgatcccc atcgccctta tcgcgttgtc gttcgtgtca tgcagcgcaa
      361 attctgaaag caacaccttc aatcaaggcg gcggtggagg cggtaacgaa gaaggtggca
      421 ccgcgaccga cggaagcgcc gcaaacagcg gtggacccct gttcgaagct tccacgggcg
      481 atggccagct cgatgaggaa agtgcctgcg tgggacaatc cttcccggga aagctggctc
      541 ccctcgacgt ttacattctg ctcgatgcta ccggttcgat gcagggctcc aacaattctc
      601 ccgtggtctg gccgagtgtc gtggccgccc tcaaagacat catctcggac ccgttgtcgt
      661 cgggtatcgg tgtgggtctg acctaccttc ccaaccaacc tccaccaact accgtcatcc
      721 ccggctcgtg taagcccggc ggagactgcg gcgccctggg attatgtgaa ttcataggaa
      781 tcgcctttgc ctgcaccagc gcctgcacca aaaacgaaga ctgcggattg tatggcccgt
      841 gtatgccctg gatgacgcct aaaacgccaa atgcaagagt ctgcaacggt gccctcgttc
      901 ccaaagtctc ctgcgatccc ctggattatg ggcaacccgt cgtccccatc gcgcccttgc
      961 ctggcaacaa agacgctttg gtccaggcca tcaacgacaa agaccccgat ggcgatgcca
     1021 cgcccaccca gccttccctc gaaggtaccc tcatctacgc caaacaatac gccaaagaca
     1081 acccaactca cctcgtccac gtcttgttcg ccaccgatgg agaacccaac aactgcacct
     1141 tcaacaccat cgacggcgct gccgtggccg ccgccaccgc tttttccaat cccccttccg
     1201 ttcccacctt cgtgctcggt atcggtgact tgaaggacct caacgcgatc gcgaaagctg
     1261 gcggaactgg cgacgcctat ctcgccaatg gctccaccgt cgcctcacaa ctcgtcaccg
     1321 tcttcaacga aatccgcgcc agcggcgctt gccaattcca gatccccgag cccgagtccg
     1381 gccaaaccct cgacttcgat cgcgtcaacg tctactacac acccctggct tcctccgaaa
     1441 aagtgcccgt caaatacgtg aatgacgccg cctcctgcga tcccaaagaa ggcggatggt
     1501 attacgacga tccttccaaa acgaatccca ccaaaatcct gctttgcccc gccacctgtg
     1561 aaggcgtcaa actcagcgaa gaaggcgtcg aagtgctgct cggttgcaaa acaatcctca
     1621 agtaatagga gggaccaaaa gcgccgtcgc ggcatccaac cgactccatg catacctctg
     1681 tccaggccat cgtctttttc cttgtcgctt tccttgtcgt gatgggttgg cacgcgtatc
     1741 tgtggaaccg gctcttgcgt gatacacagc ctcccccgcg gtggaaagtg gccggaaaga
     1801 tagccctgct gaccctcgtc accctttttc ttttcgccat gatcggtgga cgctcctggc
     1861 ctttcgactc ccttcgaccc ctgtacgcca tcgggtacgg ttggctcgga acctcttttt
     1921 tgctcgtcgt gatgctcctg ctcaccgatc ttggaaaact ttccgcgcgg ggtgttcgac
     1981 ggctcatgaa acgtgctccc accaacccgg aacgacgtct cactctggct cgcatccttg
     2041 cgggaagcgt tgctttcacc ggtttttcca gcacggcaaa aggagctgcg tccgccttgg
     2101 gaaccattcc catccgacgc atccgtgtct ccttgccacg tttgtcttcc tcgatgaacg
     2161 ggacccgtat cgttcagatt tccgacttgc atatcggtcc gattctcaaa cgctcctggg
     2221 tcgaaagcgt cgttgcccag gtgatgtccc tcgagccgga cctcatcgtg atcactgggg
     2281 atctggtgga tggtacggtc gaaaacctgc gtgatgacgt ggcaccgttg gcaaaactca
     2341 aagccaaagc tggcgtgttc ttcgtcacgg gaaaccacga gtattacagc ggcgctccgg
     2401 catgggtcga gcatgtccaa caactcggga tccgcgtgct gcaaaatgaa aatgtgccca
     2461 tcggaaaagg ggatgacgcg ttttatctcg ccggagtcaa cgacttcgaa tccgctcgac
     2521 ataaggtggg acacgcccat gacgtcgcgg cttccgtgcg aggaatccct ccgaaccgcg
     2581 aaatcctgtt gttggctcac caacctcgaa gcgtatggga tgcagccaaa cacggtgtcg
     2641 gattgtagct ttctggtcac acccatggtg gacagatttt cccctggacc tgtttcgtaa
     2701 ccttgcaaca acccttcgta tccgggctgc atcaggtgca aaacacatgg ctgtatatca
     2761 gtcgtggcac gggcttttgg ggacctccca tgcgaattgg cgcgcccccg gagatctcgc
     2821 tcatcgaaat ctatcgcgca tgaaccggga cttcccccag atggttgtgt ctcgcgtcaa
     2881 tgatgtaact atttgaaata taacgatata tttagaattt tcgcggtgga tgccccctcg
     2941 attcgaatcc cccctgttgc gttcaaccca ccatgcgaac tcccgtgatc gacgcgcgat
     3001 tcttcccgct cgtcttactc gaataaagtt cttcgtccgc tcgacggacg atcgagaatt
     3061 cgtcgtcgct cggttgagcc atggtggctc ctaccgacac tgtcgttcgc aattcgatgc
     3121 tgtggtatgg cacggcggtg gcttcgatca acgcgcgaaa cctctcggca gtttgcgcca
     3181 atcccacgga attcacattc gatacgatgg cgatatattc atcgcctccc caccgaccct
     3241 tgacatcgaa cggacgtgac gtttcgagca acgttttggc caccgccttg aggaccatgt
     3301 ccccggcttc gtggccatga tgatcgttga tggttttcag atcgtcgatg tctagcaaca
     3361 ccacaccaaa cggtatgcct ccatgtttga gttggctgca catcgatcgc agggtgatgt
     3421 ccacatagcg acgattggcc aggtttgtga gtggatcgag gaaagacaac cgttcgagtt
     3481 cacgaacacg ctgtgccaaa gccaggcttg cggtgccttc gcgaaacact tgtagtgcgc
     3541 cgatgacgtc ctgttgctga ttcaaaagcg gtgcgaaacg aacgtgaaca gggactcgat
     3601 gcccattttt gtgtcgaatg aacaaatccg catcgttcgt tcgacgatct cgcaaggctt
     3661 gtcctagcat gcatcccgat tgacacgtgg gacgtccacg atcatcgaca tgaatcagaa
     3721 tccccgcgct gcattttttc cccagcactt cctgcgcagc aaaccccgtc aactcctctg
     3781 ctccatgatt ccagtacgtg attcgacgtt ggagatcgaa ggcgcacacc gcttccgata
     3841 agttatccaa aatggcttgc actacgttcg gatcgaggag gctctccatg ggtcatgctg
     3901 tatcgtgcgt ttcaacgttt gaacatgcac gaaccagcgc cgttcgtcga gcccaggagc
     3961 gatgcaattt gcatgttcat cctttcgaaa tcgctcgttg cttgcgacac tatccgtggc
     4021 acaagctttg gccgcgtcgc tgctcactcg cttacgacac gtgcctcatc gccctggatg
     4081 acaagcgctt gcgcggcacc tcccccataa taggaagcga gtcccacatc gattctccac
     4141 acgcgatcgt cgcaagccga tgtaattcca cctttttggg gtgtgtggcc gacgaccatg
     4201 cgtttggctt ccagatgctt caacgccgtc gcaagcaccc ggcaatcccg cgcatccaca
     4261 tgggcggtcg aataccgacg attccacgtg ggcgcatctt cgctgagcat gagggcagga
     4321 agacgagggc gctcggcgcg catccaggct ttcacctctt cattgattct tccaagcccg
     4381 taacggatat gtttggataa tatcccgcca tgcacgaata gcgtatcgtt gaccatcacg
     4441 acggtattgt gacgagcaag gcgcaatgca taggggcctc ctggtgcgaa agccagcgct
     4501 cgagcacgct cctgaggttt gaatggcgcg gcgaatgcgg gattgtccgg cgatgtcacc
     4561 gactcgaagg tcgtgaagcc cgctcgcgtc acataacgca aatcaccagc cacattcatc
     4621 acctcatggt tcccctgcag ccgaaggacg agccctccag ccgccttcgc ttcctgctcc
     4681 agacgatcca acaaatccaa tatcgcgcgt tcgtcatcgc ctcgatccag gacgtccccc
     4741 gtctgcacca atacggtatt ccctccactc cagctatcct tgtcgctcat cacccccgca
     4801 acccgtagcg cctttcgggt tgccgcgaga tctccgtgca gatcaccgat tgccacgatc
     4861 cgtccggctg ccacgaaccg ataggatctg tccgtatccg tgacgtctgc ctcggtggaa
     4921 accgcgggag ctgcagccga agagacgtat ttgggttcgg aaggggtgcg ctcacacccg
     4981 gcaaccaagg gaaccaggcc gaaaaaaaca tagcgaaacc aaagcgccat ggcctgtcac
     5041 gctaggagca accgacgaac agcgcaacgc tccagtcgaa ttgatacgtt gccgcaccaa
     5101 actgcgaccc tgcacttcgc gttcggttgg ccgagtgttg ttttcggttc cccaattgcc
     5161 gacatgcgcc cttgtcaccg acggcgagcc atcagttaca aatgcaatca atcaactctt
     5221 tgcgctcttt gcaggccgtg gggtacttcg tacagcaagt cgcctgacaa gacgagaccc
     5281 cttggcactg cgatcggcaa ttagagtagt cttgggcatc cgtggcgtat tggtagtcgt
     5341 agtattccgt ggcgcacggt gagctattcg cgcactgttg acaatcgtaa ctcatgcctc
     5401 cccccgcaca ggcgctcgag cactgcgtta tacaggaagc gttatcatat ccgcaaacgc
     5461 agtcccagta gatcaacgct gcatcgcacg cctgtctgta cgaactacag cattggttgc
     5521 gacacgattg gtcactgcag tcatttacgc aatttgtgaa ctggctagcc tgtggtgctg
     5581 agagcgcctt gcttcgaacg gtaccgcaaa cagatgtttc gcacgcaaag cattctgcgg
     5641 tggcgctgcc accagcgcac gcactcgcac aagatgcgga acaatcgatc ggcgcggagc
     5701 caccagtgcc gccgtatccg cctccgccgg tgccgccaga gccgccgtat ccgccaccgc
     5761 cggtgccgcc agacccgcca gagccgccgt atccgccacc gccggtgc
//
LOCUS       DAOSOR010000375         5799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13963_length_5799_cov_4.463788, whole genome shotgun sequence.
ACCESSION   DAOSOR010000375 DAOSOR010000000
VERSION     DAOSOR010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5799
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13963_length_5799_cov_4.463788"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..302)
                     /locus_tag="PK710_17520"
     CDS             complement(72..302)
                     /locus_tag="PK710_17520"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOT11582.1"
                     /translation="MKVGPNEPCPCGSGKKYKKCCWLKDRETEAEAVRENRNVGAVAA
                     DVPKAKPESFAPANRSKGKKTVRSDAPRRRAV"
     gene            complement(393..4400)
                     /locus_tag="PK710_17525"
     CDS             complement(393..4400)
                     /locus_tag="PK710_17525"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT11583.1"
                     /translation="MGGRGPVRWACSRPLVPLRFFVIVGALLSGRYRVEQRVGTGGMG
                     EVYRATDTSTQAVVAIKVLQAKARKIDRKRFLREIQILKDLRHPGVVHYVDHGVTSDR
                     RLFLVMEWLDGEDLAVRLARSQVGLKDAVEIVRRASQALAAVHARGIVHRDLKPSNIF
                     IIRRGRSHAIKLIDFGMVKVPESEETFTRQGAMLGTPWFIAPEQAKGNPVDARADVYS
                     LGSVLFRLVTGRNVFESTHLIAYLGRLVLEDAPRASLIRKDIPVELDDLVERMLRRNP
                     AERPADAGELSRLLARLPHLSNELPRLVPSVRPPPLEQTLGGMVHGRLVSSPPGPGKI
                     LLGTVESRVVAVLFAQLPMAMLAASNARRFLSIVGDRARFETLRDGQFVAALGLDKTV
                     GDEVVRAARGALFLSTSVPGAHIALAIGRAVAGRQGLAGEALDGAALQLSYLQTPGIR
                     CDVSSCSLLDGHFVLEEDANGARLLREDIVDHEPRPLVGKTTPIVGRTREVESVIEVF
                     DEVQRAKQPHLVVVTGPAGSGKSRIRKEIVRRLRAQLPAIDVLLARGDPTVSYRGLSD
                     VGRALRARMGIRDGQTPDRQAMKLVRYVSARPGFPEGGIDFLSEFVGLTPFEDSQALR
                     IARETPPIMAARVFHTIEVLCRLDAEIAPQLLILEDFDKVDAGSVALVEWLLECKGLA
                     LTVIALGRPAVDSRFPQLWEEGRPVTRLRIGPLSREACEQIAQMALAEGSESMVQRLV
                     QQAHGNPLFLEELIRHAAAGRRSLPLSVQSLIQTRVDELEESQRQVLRAASVYGDVFW
                     TEGVCALLDRDCEAELLELVQQEILVRHRVTRVHGHDQWSFRHDLVRDTVYASLLEKD
                     LVRYHRRAAEWLKLVGAPDPAALARHAEARSDRDQAVRLYSKACSQAFGKGQLEAALE
                     FSTRGAACADDPLVRAECLLQRAQILSWMCRFSEQREAANAAAMLASSGSDLWGEARR
                     LAATALREEGSVSEADAMMTATLQKSRAAGLSFATRSRLLSEWARCLVEIGRAKEALA
                     VAERALVQAQRAGPRAFHAHLRALDARSLAISYLGDYSASIEATDAVVKLADQLGDMM
                     QATRSRINLGFALIRVGCIDQGQVCIDRALNDARLLRMPAGEGFALHNLGVIRARQRR
                     LDEAIDIMMQAIAIGQRIGYQRLTLLSRLYLAMFLAWRRQGDDVEQAVTVMQATREES
                     AAFPFADKELALADAQVARARKDPETALRRCKDYFERSSSLEALEDGEEGLHLTQLLV
                     LLDLGREQEADLAARQAYDCVTARCARLSNNTHREAYLSNLFECRKILDIASDRLSLT
                     RPLISLPPPAPSDAQ"
     gene            complement(4453..5472)
                     /locus_tag="PK710_17530"
     CDS             complement(4453..5472)
                     /locus_tag="PK710_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT11584.1"
                     /translation="MTGASADNASMKRRPLLQVERLSKLFAVSSGLWGKPSFARAVDD
                     VTLHVRHAETLALVGESGCGKTTLGRCILRLIEPTFGRIVFEGKDLSPLSQRQLRPFR
                     KQMQIIFQDPYASLNPRMRIGDIVAEGIRIHRLASGSEVMDRVVAVLDSVGLSRDVIM
                     RYPHEFSAGQRQRVGMARALAVEPSFMVCDEPVSALDVSVRAHILNLLQDLQEKRKIA
                     YLFISHDLGVVRHLSHRTAVMYLGKIVEVGPTDDVFSGPWHPYSRALLSAVPVADPER
                     KQLRMVLEGEVPSPISPPSGCPFHPRCPKNDGVDCVQRMPPLREMPEGVHHRVACWHP
                     EGRTM"
     gene            complement(5469..>5799)
                     /locus_tag="PK710_17535"
     CDS             complement(5469..>5799)
                     /locus_tag="PK710_17535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT11585.1"
                     /translation="VAGFAEHVLVMYAGQIVESSPVKELFRSPLHPYTQALIQCVSAV
                     GVDGTTRHTRLPLVEGRIADIESRPSGCRFRPRCPRAHEACLEDPPWVEKAGRGARCV
                     LLSEKEP"
BASE COUNT         1289 a         1952 c         1458 g         1100 t
ORIGIN      
        1 aattgccatg gttcgtcctg taccagggag taggtgccga ggctagccgc ttgcggcccc
       61 gcaggtcgga atcatacggc ccgacgacga ggggcatcgc ttcgaacggt ctttttgccc
      121 ttggatcgat tcgcaggggc gaatgactcg ggttttgcct tgggcacatc ggcagccacc
      181 gcgccgacgt tacgattctc gcgaaccgct tccgcttccg tttcgcgatc tttcaaccaa
      241 cagcattttt tatacttttt tccggagcca caagggcacg gttcattggg accgactttc
      301 atgggtagcc tcgtgggttg cgccaacctc atgctatttg acgtgctttc cctggtgtcg
      361 tcaagcacgg cccggcaatc gcctcgtgca acctattgcg cgtcgctcgg cgctggaggg
      421 ggaaggctta tcaacggacg ggtcaacgac aggcgatcgc tggcgatatc gaggattttt
      481 ctgcactcga acagattgga aagataggct tctcgatggg tattgttgct gagccgggca
      541 caccgggccg tcacgcaatc atacgcctgc cgggccgcca aatccgcttc ctgttcccga
      601 ccgagatcca acaagaccag cagttgcgtc agatgcaacc cctcttcccc atcttccaat
      661 gcctccaaag aggaggagcg ctcgaaatag tctttgcaac gtcgaagggc agtttccgga
      721 tccttacgcg ctcgagccac ttgcgcgtct gccaacgcca actccttgtc tgcaaacgga
      781 aaagccgcgg actcttcccg cgtcgcttgc atcacggtca cggcctgctc cacatcgtcg
      841 ccttgccgtc gccaggcaag aaacattgcc aaatacagcc tagaaagcag ggttagacgt
      901 tggtaaccga tgcgttgtcc aatcgcgatg gcctgcatca tgatgtcgat ggcttcatcc
      961 aagcgccgtt gacgggctcg gatgactcca aggttgtgca aagcaaatcc ttctcccgca
     1021 ggcattcgca gcaggcgcgc atcattcagg gcccgatcga tacacacttg gccttggtcg
     1081 atgcaaccga ctcgaatcag cgcgaaaccg agattgattc gcgatcgcgt ggcctgcatc
     1141 atgtcgccga gttggtccgc cagcttcacc accgcatcgg tggcttcgat gctggccgaa
     1201 taatctccaa gataactgat ggcgagactg cgtgcgtcga gcgcccgaag atgcgcatgg
     1261 aaagcacggg ggcccgcgcg ttgcgcttgc accaacgcac gctccgccac ggcaagcgcc
     1321 tccttggctc ttccaatctc caccaaacac cgagcccatt ccgacaacaa ccgcgagcgt
     1381 gtcgcaaacg acaaccccgc cgccctcgat ttctgcagcg tcgccgtcat catcgcatcc
     1441 gcttcgctca cactgccctc ttctcgcaaa gccgtcgcgg ccaaccttcg agcttccccc
     1501 cataaatccg accccgaact cgccaacatc gccgccgcgt tcgccgcttc tcgctgctcg
     1561 gaaaatcgac acatccacga caatatctgt gcccgttgca gcaaacactc cgctcgaacc
     1621 agaggatcgt cggcacacgc cgcgccgcgt gtcgaaaact cgagggccgc ctccaactga
     1681 cctttgccaa acgcctggct gcaagctttg gaatacaacc gcaccgcttg atcgcgatcg
     1741 cttctggctt ccgcatgcct cgccaacgcc gccggatccg gagcccccac cagtttcaac
     1801 cactccgcag cacgacgatg ataacggacc aaatcttttt caagcaacga cgcgtacacc
     1861 gtatcgcgaa ccaggtcgtg acgaaaggac cattggtcgt gaccatgaac acgcgtgacg
     1921 cgatgacgca caagaatttc ttgttggacc aattccaaca actccgcttc gcaatctcgg
     1981 tcgagcagcg cgcatacccc ttccgtccaa aaaacatcgc catacaccga tgccgcccga
     2041 agcacttgcc gctgcgattc ctccagttca tcgactcgcg tttgaatcaa cgactgaacc
     2101 gaaagaggca gggaacgacg acccgcggcc gcatgacgaa tgagttcttc caaaaataac
     2161 ggattgccat gcgcctgctg aacgagacgc tgcaccatgc tctccgagcc ttcggcaaga
     2221 gccatctgag caatttgctc gcaggcctcc cgagacaatg gcccaattcg cagccgcgtc
     2281 accggacgcc cttcctccca caattgcggg aaacgactat ccaccgcggg acgtcccaac
     2341 gcgatcacgg tcaacgcaag ccccttgcac tccaagagcc attccaccag ggccacggaa
     2401 ccggcgtcca ccttgtcgaa gtcttccaag atgagcaact gcggcgcgat ctccgcatcg
     2461 agacgacaca acacttcgat ggtgtgaaat actcgtgccg ccatgatggg tggagtctcc
     2521 cgcgcaattc tcaacgcctg actatcctcg aacggtgtca acccaacgaa ttcactaagg
     2581 aaatctatcc ctccctccgg gaatcccggt cgcgcggata catatcgaac cagcttcatc
     2641 gcttgacgat ccggcgtttg cccatcgcga atccccatgc gcgctcgtaa cgcacggccc
     2701 acatccgata acccccggta ggaaaccgtc ggatctccgc gcgcaagaag cacgtcgata
     2761 gccggtagct gcgcgcgaag gcgacgaacg atctccttgc gaatgcggga tttgccactt
     2821 cccgctggac ccgtaaccac aaccagatga ggctgttttg cccgctgcac ttcgtcaaaa
     2881 acctcgatca cagactccac ttcccgcgtc cgtcccacga tgggtgtcgt ttttcccacc
     2941 agcggacgag gttcatgatc cacgatatcc tcccgcaaca acctcgctcc gttggcgtcc
     3001 tcctccaaca cgaaatgccc atcgagaagg gagcaagacg acacgtcaca gcgaatgccc
     3061 ggcgtctgca agtagctcaa ttgcaacgcc gcgccatcga gtgcttcccc cgcaagccct
     3121 tgacgccctg ccaccgctct tcctatggca agcgcgatat gggcaccggg aaccgaggtg
     3181 gatagaaaaa gtgcgcctcg cgccgcccgc accacctcat cgcccaccgt cttgtccaac
     3241 cccaaagcag ccacgaattg accgtcgcgc aacgtctcaa aacgcgcccg gtctcctaca
     3301 atcgaaagga aacggcgggc attggacgcc gccaacatcg ccatcggaag ctgcgcaaac
     3361 aaaaccgcca ccacgcgact ttccaccgtc cccaacagga tttttcccgg acctggaggc
     3421 gaagacacca gcctgccatg caccattcct ccgagcgtct gctccaaagg cggaggacga
     3481 acactcggca caagacgcgg caattcgttg gaaaggtgcg gcaaacgcgc caatagcctc
     3541 gacaattcgc cggcatccgc cggacgctcc gcggggttgc gccgaagcat tcgctccacc
     3601 aaatcatcga gttcgacggg aatgtctttt cgaatcaatg acgctcgcgg cgcatcctcg
     3661 agcaccaacc gtcccaagta ggcaatgaga tgcgtcgact cgaatacgtt gcgccccgtg
     3721 accagacgaa atagcaccga accgagcgaa tacacatccg ctcgcgcatc taccggattt
     3781 cctttggctt gttcaggagc gatgaaccac ggcgtgccaa gcatcgcccc ctgtcgcgta
     3841 aacgtctctt ccgactccgg tactttcacc attccaaaat cgatgagctt gatggcatgg
     3901 ctgcgcccac gacgaatgat gaatatattc gagggcttga gatcccggtg gacgatgcct
     3961 cgtgcatgca cagcggccaa cgcttgagag gcgcgacgca cgatctccac cgcgtctttc
     4021 aagccgacct gcgatcgagc caaacgcact gccaagtcct ccccgtcgag ccattccatg
     4081 accaaaaaca gccgacgatc cgaggtgact ccgtggtcca cgtaatgcac gacgcccgga
     4141 tgcctcaggt ccttgagaat ttggatctct cgcaaaaaac gtttgcggtc gatctttcgc
     4201 gctttggcct gcaacacctt gatggccacc accgcctgcg tggaggtatc cgtggcgcga
     4261 tatacctccc ccattccccc ggtaccaacc cgctgctcca cacggtaccg acctgacaac
     4321 aatgctccca cgatgacgaa aaatcttaga ggaacaagcg gacgtgagca agcccagcgc
     4381 acgggccctc gaccacccac gacggctttc ctactgccta tcccctttgg attggctcgc
     4441 tcgttacctt cattacatgg tcctgccctc gggatgccaa caggctaccc gatgatggac
     4501 cccttccggc atttcacgca gcggtggcat tcgctgcaca caatcgaccc catcgttttt
     4561 tgggcaacga gggtgaaatg gacatcccga cggaggcgat atcggactgg gtacctcgcc
     4621 ttcgagcacc atgcgcaact gctttcgttc cggatccgcc acgggaaccg ccgatagtag
     4681 tgctcgtgaa tacggatgcc atggccccga gaacacgtcg tcggtcggtc ccacttccac
     4741 gatttttccc agatacatca ccgccgtgcg atgactcaga tgacgcacga cccccaagtc
     4801 atgagaaatg aataaatacg cgatcttacg tttttcttgc aggtcttgca gcaggttgag
     4861 gatgtgcgcc ctcaccgata cgtcgagggc gctaaccggc tcatcgcaaa ccatgaacga
     4921 aggctcgacc gccagagcgc gcgccatgcc cacacgctgt cgttgccccg cggaaaactc
     4981 atgcggatag cgcatgatca catcccgcga caaccccacg gaatcgagaa cggccaccac
     5041 cctatccatc acctccgaac cggatgccaa tcggtggatt cgaatgccct ctgccacgat
     5101 atccccgatc cgcatcctcg gattcaacga tgcgtacggg tcttgaaaaa tgatctgcat
     5161 ctgctttcga aacggacgaa gctgccgctg cgacaaggga ctcaagtcct ttccttcaaa
     5221 cacgattcga ccgaacgtcg gctcgatcaa acggagaatg cagcgtccca aggtagtctt
     5281 tccgcatccg gactccccca ccaacgccag cgtttcggca tgacgcacat gaagggtcac
     5341 gtcatcgacg gctcgtgcaa acgacggttt tccccaaagc ccggaggata ccgcgaagag
     5401 cttggacagg cgctcgacct gcaacaaggg gcgacgcttc atcgaggcat tgtcagcgct
     5461 ggcgccggtc atggttcttt ctccgaaagc agcacacatc ttgcgcctcg ccccgctttt
     5521 tcgacccacg gaggatcctc gagacacgcc tcatgcgctc gtggacatcg cggacgaaaa
     5581 cgacaaccgc taggacgtga ttcgatatcg gcaatgcgcc cctcgacaag cggcagcctt
     5641 gtatgccgcg tggtgccatc cactcccacc gcggacacgc attggatcaa agcttgcgtg
     5701 tagggatgta acggtgaccg aaatagctcc ttgaccggcg aagattccac aatctgcccc
     5761 gcgtacatca ccagcacatg ctcggcaaac ccggccacc
//
LOCUS       DAOSOR010000376         5797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_13973_length_5797_cov_4.582550, whole genome shotgun sequence.
ACCESSION   DAOSOR010000376 DAOSOR010000000
VERSION     DAOSOR010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13973_length_5797_cov_4.582550"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="PK710_17540"
     CDS             <1..568
                     /locus_tag="PK710_17540"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOT11586.1"
                     /translation="VEVTVPSPVGAVIFVDGIDMGPAPFKGELTVGRHTIEARAAGFV
                     TARQTSDVHWNQPVRLTLSLSRERHDGKVRIRVSEADATIMIDGKAVGRGSWEGILPT
                     GGHQLMVKKDGFETYTSDIALSDDQVRTMTVPLVREARGAAWVWWTVGSVAVLAGGAL
                     ATYAIAKPTEQAPIIGTWAPGNVPTGFR"
     gene            591..2546
                     /locus_tag="PK710_17545"
     CDS             591..2546
                     /locus_tag="PK710_17545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11587.1"
                     /translation="MLMEITHRPFLFYPKVKKAIRTVGCGALIAVSLGCSPDEPTQLV
                     AGMTTQMRVPDEIKSVGVVVQSSGRLVFCDSYPVSDGSVTLPSTLATSAENSPNDPVT
                     VTVLGFGRDQSSFSADCVVRQPDVGEDGVTVMRRRRSTYLENRALYLPMPIKHACVDV
                     SCPQGETCVGGLCQKMDVDPSKLVDFDDRLVFGKTNTCFDVGGCLPPTKSIPAQLVDA
                     DTCEFRIDIPQGASVPAHGSLNVRIVHENFTSEVLDLDEAEGFVLVDDPTGTRFRLAP
                     SLCTSRYKTGKILGVWATKFCASKTPLQPICEDDLQEIVSGESSLYGPAACVSGSRLV
                     PTESALYVLMDRSDSMRAYFGPDGLHQLLSLSLEDPVFERTRVAFSLLPGQASDCGEA
                     TNRFAAPTGPEGVPFMFAKDAREAVAPIIANEGNVWNSDSPVMFDAALSAHGAYAALK
                     NLTPTAPTKDFNRRAVLVLGNRDFLSHCAGGSDLLDLVSAARAEGLYTYFVVLKAPPA
                     TDQQGRDPLADAAQLALAGGTEVFDATQDPNAGALALQTITADLGSCLYDAPEGVAVV
                     NDIAHRTVTYLNPLTNVRETIPYNEHCTEQVSGDSGWNTDALGRIRICGYACETLRDT
                     AKMLSVSAAQSGQPVAVLPIHLTEPCK"
     gene            complement(2548..3888)
                     /locus_tag="PK710_17550"
     CDS             complement(2548..3888)
                     /locus_tag="PK710_17550"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOT11588.1"
                     /translation="MRTTIIALHRSGRCSYVRISVIGSILAAAPMAVAQPTTPTEPPP
                     TNTVMPAEKTGRLIVREKNGFPIRVLIDGVDRGITPLTLDLPPRNYEVSGIASTVVAA
                     AQQISLQENQRLDIELEAVPAMARVKIHTSDGHGTIQIDGRTLAEGAYEGELPVGEHI
                     VRVERDGFEPWEKTIQLAHQQVIVEAVTLDRKADVLHAHGASHRVKDGLYGGFHVGLA
                     LMPAGADSTLDTGCASIGATECESSKPWGLGLQGYLGYSLEPLGFELLLGTLGDYANP
                     KAHFDGKHGSSVNPLVAQPAREEEFEIFRAGVLGAIRVRGSVDIAPVRLTGALGAGLS
                     WKMMGMTRSVTTTDGSNGTNRYLPDAVSYWSPGVTMDLGGQFMIGETTTVSAGVWFWA
                     ETASDKARTQSESKRVIVSPQENTPPRPIATPAYDLANGAQLFVGPYLGMQFGP"
     gene            4403..5008
                     /locus_tag="PK710_17555"
     CDS             4403..5008
                     /locus_tag="PK710_17555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11589.1"
                     /translation="MRNLVFLIGAMGMLVVGCGAGTSPEPQTATPPVVVESAQEPEAT
                     EGAQEPTGNQQTPELGEDVAVRPVPGPSVESEPSAPPPPPPPEPARPSDVPGANMHIQ
                     TVTINGVTVQDVECRTQGGGLGGLLGTMTVGKPFADKKAALDRCVQGRQLVRVRWEGV
                     GGKMTKVTVLSTPDNACVVRTLNGAASTIEGVCAASIELGK"
     gene            complement(5236..5318)
                     /locus_tag="PK710_17560"
     tRNA            complement(5236..5318)
                     /locus_tag="PK710_17560"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5283..5285),aa:Leu,seq:caa)
     gene            5468..>5797
                     /locus_tag="PK710_17565"
     CDS             5468..>5797
                     /locus_tag="PK710_17565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11590.1"
                     /translation="MTHSISSFWPLLLVVPLSGCGGRTLEAESNPDAGVADSATPGKG
                     GSGGGANGGSGGAENVGGSGESGGSIAGGTGGGPGGSGGIEDGGPAGSGGSTPAPCTL
                     STGDPSCD"
BASE COUNT         1140 a         1458 c         1927 g         1272 t
ORIGIN      
        1 ggtggaagtg acggtgcctt ctcccgtggg ggctgttatt ttcgttgatg ggatagacat
       61 ggggcctgct ccattcaaag gggagctaac ggtggggcgc cacacgatag aggcgagggc
      121 tgcgggattc gtcacggcgc gtcaaaccag cgatgttcat tggaatcaac cggtccgatt
      181 gaccttgtcc ttatcgcgcg aacgtcacga tgggaaagtg cgcattcgtg tcagtgaagc
      241 cgatgccacc atcatgatcg acggcaaggc cgtggggcga ggatcgtggg agggaatctt
      301 gccaacgggg ggtcatcagc tcatggtaaa gaaagacggg tttgagacgt atacgagtga
      361 tattgctttg agtgacgatc aagttcgaac gatgacggtt ccgttggttc gggaggcgag
      421 aggggccgca tgggtttggt ggacggtagg gtcggtggcg gtattggcgg gcggcgcgtt
      481 ggcgacctac gcgatagcaa aaccaacgga acaggcgcca atcattggca cgtgggctcc
      541 cggcaacgtg cctacgggtt ttcgctgata aaattaccac ggagaatcaa atgcttatgg
      601 aaattaccca tcgacctttc ctgttctatc caaaggttaa aaaggcaata cgaaccgtcg
      661 gttgtggggc attgatagcg gtcagcctgg gctgttcgcc cgatgagccc acgcagctcg
      721 tcgcgggaat gaccacccag atgcgcgtac cggacgaaat caaaagcgtg ggcgtcgtgg
      781 tgcaatccag cgggcgcctt gtgttttgtg actcctatcc tgtctcggat ggctccgtga
      841 cgttaccgag caccctggcg acgtcggctg aaaacagtcc gaacgatccg gtcacggtga
      901 cggtgctggg ttttggccgg gatcaaagca gtttttcggc agattgtgtc gtgcgtcagc
      961 cggacgtggg ggaagacggg gtcacggtga tgcgtcgtcg ccgctctacc tatctcgaaa
     1021 acagagcatt gtatttgccg atgcccatca agcatgcgtg cgttgatgtg tcatgtcctc
     1081 agggggagac gtgtgtgggc gggttatgtc agaagatgga cgtggatcca tcgaagctgg
     1141 tggatttcga tgatcggctg gtgtttggga aaacgaatac atgttttgat gtggggggat
     1201 gtcttcctcc caccaagtcg attcccgcgc agctcgtcga tgcggatacg tgcgaatttc
     1261 ggattgacat accgcagggg gcgtcggttc ctgcccatgg ctccttgaac gtgcggatcg
     1321 tgcatgagaa ctttacgtcc gaggtattgg atctcgatga ggcggaaggc ttcgtactgg
     1381 tcgacgatcc cacggggact cgttttcgtc ttgcgcccag tctatgcact tcgcgttaca
     1441 agacgggcaa gatacttggg gtatgggcga cgaagttctg tgcatcgaag acgcccttgc
     1501 agccgatttg cgaagacgat ttgcaggaaa tcgtatcggg agaaagcagt ttgtatgggc
     1561 ctgcggcgtg tgtatcggga agccggttgg ttcctacgga gagtgcgctt tacgtattga
     1621 tggatcgatc ggacagcatg agagcgtact ttgggcctga cggtttgcat caattgctct
     1681 cgttatcgct cgaggatccg gtatttgagc gaacacgggt ggccttttcg ttgttaccgg
     1741 ggcaggcctc ggattgtggg gaggcgacga atcgttttgc ggcgcctacc gggccggagg
     1801 gggttccgtt tatgtttgcc aaggacgcaa gggaagcggt ggcgcccatc attgcgaacg
     1861 aagggaatgt gtggaatagc gactcgcccg tgatgttcga tgcggcgcta tcggctcacg
     1921 gtgcctacgc ggcgctgaag aacctgacac cgacggcacc caccaaagat ttcaatcgtc
     1981 gagcggtgct ggtccttggg aatcgggatt tcttgtcgca ttgtgccggg gggtcggact
     2041 tattggacct tgtatcggcg gcgcgggcgg aagggttgta cacgtatttc gtggtgctga
     2101 aggcgcctcc ggccaccgac caacaggggc gagatccact tgcggacgct gcgcagcttg
     2161 cgttggcggg aggcacggaa gtatttgacg ccacgcaaga tcccaatgct ggtgcgttgg
     2221 cgttgcagac gatcacggcg gatttgggga gttgtttgta tgatgcgccg gaaggggtgg
     2281 ctgtggtgaa cgacatcgcc catcgcacgg ttacgtactt gaatccattg acgaatgtga
     2341 gagagacgat tccgtacaac gagcattgta ctgagcaggt ttccggcgat agtggatgga
     2401 atacggacgc cttggggcgg attcgtattt gcggctatgc ttgtgaaacc cttcgggaca
     2461 cggcgaaaat gctatctgta tccgcggcgc aatcgggaca gcccgttgcc gttcttccca
     2521 ttcacttgac ggagccctgc aagtaagtca ggggccgaac tgcatcccca agtaaggtcc
     2581 cacgaagagc tgcgcgccat tggcgaggtc gtacgcaggt gttgcaatgg gtcttggggg
     2641 cgtattttct tgtggtgaga cgatcacgcg tttggactca ctttgggtcc ttgctttgtc
     2701 tgatgcggtt tcggcccaga accacactcc ggccgagacg gtggtggttt ctccaatcat
     2761 gaactgcccg cccaagtcca tggtgacacc tggcgaccaa taactcacgg catcgggaag
     2821 atagcgattc gtgccattgc tgccgtcggt ggtcgtcacc gaacgggtca ttcccatcat
     2881 tttccaggac aatcccgcgc cgagggcgcc ggtgagacga acgggagcga tatcgacgct
     2941 gccacgaaca cgaatggcgc ccagcacgcc agcccggaag atttcgaatt cttcctcccg
     3001 ggcgggctgc gccaccagag ggttgaccga cgacccatgt tttccatcga agtgtgcctt
     3061 ggggtttgcg tagtcaccga gggttcccag caacaattcg aagccgaggg gttcgaggga
     3121 ataaccgagg tatccttgca aaccgaggcc ccaaggtttg gaggattcac attcggtggc
     3181 gccgatggaa gcgcagccgg tatccagggt ggaatccgct ccggcgggca tgagagccaa
     3241 gcccacgtga aaaccgccat acaggccatc tttgacgcga tggctggcgc cgtgggcgtg
     3301 gaggacatcg gctttgcggt ccagggtcac ggcttccaca atgacttgtt ggtgcgccaa
     3361 ttggatggtt ttctcccacg gctcgaaccc atcccgttcg acgcgcacaa tgtgttcacc
     3421 tacgggcagt tctccttcgt aggcaccttc tgccagggtt ctaccgtcga tctggattgt
     3481 cccatgacca tcgctcgtat gaattttcac gcgggccatg gcggggacgg cttcgagttc
     3541 gatgtcgagc cgttgatttt cttgtaggga aatctgttgg gcggcagcga cgacggtcga
     3601 agcgattccg ctgacctcgt agtttctagg gggtaagtcg agggtcaacg gcgtgatgcc
     3661 tcgatcgacg ccatcgatga ggactcggat ggggaatcca tttttttcgc gaacgatcaa
     3721 gcgacctgtt ttttcggccg gcatgacggt gttggtgggg ggaggttcgg tgggcgtggt
     3781 gggctgcgcg acggccatgg gagccgcggc caggatcgag ccgatgacgc taattctaac
     3841 gtacgaacac cgtccagatc ggtgaagagc aatgatcgtg gtacgcaagc tggtcctctt
     3901 tttcggcgga aaggtccgaa cggaccccct cgtgcgaccc tgagactttg gggttcccgc
     3961 gtcaagcacg taacatctag caaaacggat cccgcgcgca acttgtgaaa aactggccct
     4021 cgttggggtg tgtcttttcg tttttccgag gacgattcta ctacgatggg ggcatccgat
     4081 gccgatcccg gagcgtcgtt attgggatat ttgtcccgcc caggactgcc aacgggccga
     4141 tccggaatca cataacctat tgttattatt gaataattta gcagttcgat agcaagaggg
     4201 gggagtattg cgtgtggcct gatttcgggg gttatcgacg gtcgttggga ttccaagcac
     4261 caaagccaaa ggcgcgtggg agtgggtccg cgggggcggc gttgcgcctt tgcgtgggtt
     4321 caatcaaatg attttgcgat acaaatcgta tagatacgcc atatcgtgga tatcgagggt
     4381 agtgtgagat agcatcggca ccatgagaaa ccttgtattt cttatcggtg ccatgggcat
     4441 gctcgtggtg ggatgtgggg cggggacatc gccagagcct cagacggcaa cgccacctgt
     4501 tgtcgtggaa tctgcccagg agcctgaagc caccgagggg gcgcaagagc cgacgggcaa
     4561 ccaacaaaca cctgaactgg gcgaggatgt tgcggtcagg ccggtacctg ggccatcggt
     4621 ggagtccgag ccgtctgctc ctccaccacc gccgccgccg gaacccgcgc gccccagcga
     4681 tgttcctggt gccaacatgc atatacaaac cgtgacgatc aatggggtca cggtacaaga
     4741 cgtggaatgc cgcacgcagg gaggagggct cggggggtta ttggggacca tgacggttgg
     4801 caaacccttt gcggataaga aggcggcgct ggaccgttgc gtgcaagggc gtcaactcgt
     4861 tcgggttcgc tgggagggtg ttgggggaaa gatgacgaaa gtcaccgtcc tttcgacgcc
     4921 tgacaacgcg tgtgtcgtgc gaacgttgaa cggagcggca tccacgatcg aaggcgtttg
     4981 cgccgcatcc atcgagcttg gtaaatagcc tttaccagtc caatcatcgg gaggcattcg
     5041 atcgcgcggg gctatccatt tcggtatagt atggggaagc caccgcaggg tcggtgatcg
     5101 caaagggttc ctggtctggc agcagaggaa gcgaaacggc tctggggtcg ttcggctgga
     5161 accatgcgtt ttctcgcagt attcgctttc ggagcgaggc cccgtttttg tttgttcgat
     5221 gcgcgacgaa tcttgtgccg aaggagggag tcgaaccctc acgcccttgc ggacagtgga
     5281 ttttgagtcc accgcgtctg ccattccgcc acttcggcgt ggtgagaacg ggctcttgtt
     5341 tacaacaagc gggaccaatt gcaagagggc gatgcgcgcg ggatgcagaa tggaagcaaa
     5401 ccgcgatttt ctgggatcgc gcgcgctgtc cagcggtgct atctctgagg ggtggaggta
     5461 tggcaacgtg actcattcta tttcatcttt ttggccgttg ctccttgtgg tgcctttgag
     5521 cggctgtggc ggtcgcacgt tggaagcgga gtccaacccg gacgccggcg ttgcggacag
     5581 cgctacccca gggaaaggag gtagcggcgg gggcgccaac ggggggagcg gaggtgcgga
     5641 gaacgtcggg ggaagcgggg aaagcggggg aagcatcgcg ggcggaaccg ggggaggccc
     5701 cggcggctcc ggaggcatcg aggatggcgg tccggcgggt tcgggtggtt caaccccggc
     5761 gccatgtact ctatccaccg gagatccttc ctgcgat
//
LOCUS       DAOSOR010000377         5787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14004_length_5787_cov_4.004885, whole genome shotgun sequence.
ACCESSION   DAOSOR010000377 DAOSOR010000000
VERSION     DAOSOR010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14004_length_5787_cov_4.004885"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="PK710_17570"
     CDS             complement(<1..952)
                     /locus_tag="PK710_17570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11591.1"
                     /translation="MKRVYSIRTHGNPTLALRSLFASWLEQKVVQALLVPVRQAPSAV
                     VMQTLVSDPEELDYADPLAPVAFSNAAALLSSITHHSAGARLGAVVRSCDIRAFVERI
                     KLHQGSRDNLLLVGFDCLGRFENQEYLKLCNKYDDLTTEFYRAATPGIDGIEITRACR
                     ACVFPVAPHADINICLFGTNLWEEIRLEAATAAGVSALEALSLPLVGESPERGAVIES
                     MVSERKAYRDALLSEYRARAGSVPGFVRLTAACVNCYNCRVACPVCHCRECVFCTDLF
                     LHDGSRLLGRAKRHGALRMPTDTLLYHLTRMVHVGALCVGC"
     gene            complement(949..1386)
                     /locus_tag="PK710_17575"
     CDS             complement(949..1386)
                     /locus_tag="PK710_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOT11592.1"
                     /translation="MSASDFEPTIVGFLCNWCPFTAVDLAGSLTRAKSPNFRLIRVVC
                     SGTVDPKFVLQSFLEGADGVFIGGCWPGDCHYIHGNLKARRRVALLTEVLGQVGIEKE
                     RLWLRWIAASESARFVEMVEEMTRQLRVLGPCALARRPRGISP"
     gene            complement(1565..2848)
                     /gene="glpB"
                     /locus_tag="PK710_17580"
     CDS             complement(1565..2848)
                     /gene="glpB"
                     /locus_tag="PK710_17580"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HOT11593.1"
                     /translation="MTAHFDLAIVGAGMTGLCAAVFAASRGLSTVVLGPTGSWAHPSG
                     LFDLLGVYPLEEKKRVHNPWDALKTLMANEPSHPYGLVGEPALRDAFSFLLDVLSQRN
                     YSYFRVSEANSELITSLGTRKTTYCLPATMGAGVQAWQRKSSCLLIDFFGLKDYSAQL
                     VASNLRSAWPLLRWSTVECPGTDSQHEISPAFLARQFDEAPFRDRLAERITPLLQGAI
                     AVGLPAVLGFEHSLGALEHLQNRLGVPVFEIPCTPGFVPGLRLRAAFEKALHGLGAKL
                     MTEKVVRVRAEPPDGFVLFAGETDALTEIRAPAVLLATGRFLAGGLAAERTGVRETVF
                     NLPVWQPDARQAWHRADLFDTRGHPIHRAGVRVDALFRPVDSSQLPVFTGLFVAGSLL
                     AHQDWMRTKSGAGLAIGTAWGAVKACQRYLEEGQS"
     gene            complement(2845..4392)
                     /locus_tag="PK710_17585"
     CDS             complement(2845..4392)
                     /locus_tag="PK710_17585"
                     /inference="COORDINATES: protein motif:HMM:NF008313.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT11594.1"
                     /translation="MTALRANVLVIGGGATGTGLARDLALRGIHTVVVEKADLNAGAS
                     GANHGLLHSGARYVAGDAQSARHCYEEGQILRRLASHCLEDTGGLFAAVQGDDENYIA
                     DFPGLCARCGIPARPVDIRQAWEMEPALSKTMIAAFEVPDATVDPFKLSLENMAHACH
                     LGARFLPQTRVVGFDCRHGRIQRTHVVDTRSGIKTVIEADEVVNAAGAWSGQIAAMAG
                     ITLEILYSKGTLLITQRRFTDRVINRLRKPANADIIVPGGTVSVLGTTSLRVDDLEDI
                     APTPAEVDTIVQETAQLVPLLEKARILRAYAGVRPLVRQRCSGDDRSVSRDLSLFDHR
                     EHGVENFITVTGGKLTTFRQMAEKAGDLVCAHLGCDLPSRTAEVALPDASLWTFPGRS
                     PRLWAEAAKPKDWLLCECEMVPPSAMTHILEKLPSLPPPTLLDVALRSRVGKGACQGS
                     FCSVRVLAHLVGTRTSDASGTLPELKAFLNERWKGQRSVLFHEQARQAELNEAIHCAL
                     LGLELEP"
     gene            4742..5206
                     /locus_tag="PK710_17590"
     CDS             4742..5206
                     /locus_tag="PK710_17590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11595.1"
                     /translation="MNPQRLDTSRRIIHGVRQRGLAMAIVGGCTLFSMMGCRSKLTCE
                     AIAKRNATCSEAFVEEAKRRTRAKMAERLASLPEAERKKMVAKMEASFKESAKEVRAT
                     VESDEFLQNCRASWQDPSKMPKALKEELARCLSLPDCEGYATCFMDSAALSP"
BASE COUNT         1298 a         1657 c         1729 g         1103 t
ORIGIN      
        1 cacagccgac gcaaagagcg ccgacatgaa ccattcgcgt gaggtggtat agtagcgtat
       61 cggtgggcat ccgaagcgcg ccgtgacgtt tggcgcgacc cagaagcctc gaaccatcat
      121 gaaggaataa atccgtgcaa aaaacgcatt cgcgacaatg acataccggg cacgccaccc
      181 ggcagttgta gcaattgaca caggcggccg tcagccgcac aaaccccggc accgaacccg
      241 ctcgggcccg atattccgat agcagagcat cgcgatacgc cttgcgctcg gacaccatcg
      301 attcgatgac cgctcctcgc tcgggcgact cccccacaag gggcaaggac agggcttcca
      361 acgcggaaac acccgccgcg gtcgcggcct cgagacggat ttcttcccat aggttcgtgc
      421 cgaataggca gatattgata tccgcatggg gcgccaccgg aaagacgcac gctcgacagg
      481 cccgggtgat ctcgatccca tcgatgccgg gagtcgcggc gcggtagaat tccgtggtca
      541 aatcgtcgta tttattacat aattttagat actcctgatt ttcgaagcgc cccaaacaat
      601 cgaatcccac caacaataga ttgtcccgcg acccttgatg gagcttgatc cgctcgacaa
      661 atgcgcgaat atcacagctt cgaaccaccg ctccaagacg cgctcccgcg gagtgatggg
      721 tgatgctcga cagcaaggct gccgcattgg aaaaggccac gggggccaaa ggatccgcgt
      781 agtccaactc ctcggggtcc gagaccaacg tctgcatcac gaccgcgctg ggcgcttgcc
      841 gcaccggcac gagcaatgcc tgcaccacct tctgctccag ccaggaagca aacaagctac
      901 gcaacgccag cgttgggttt ccatgggtgc gaatcgaata cacgcgtttc atggcgatat
      961 tccccgcggc cgtcgcgcca gagcgcaggg gcccaaaaca cgcagttggc gcgtcatttc
     1021 ctccaccatc tcgacgaatc gagcgctttc ggaggcggca atccaccgca accagaggcg
     1081 ttccttctcg atgcccactt gacctagaac ctctgtcagc aacgccacgc gacgtctggc
     1141 tttcagattc ccgtggatat aatggcagtc tccgggccag catccaccga tgaatacgcc
     1201 atcggctccc tcgagaaagc tttgaagcac gaatttgggg tccaccgtgc cggagcagac
     1261 gacccggatg agccggaagt tgggcgattt ggcccgggtc agggaacccg ccagatccac
     1321 cgccgtgaag gggcaccaat tgcataaaaa tccgacgatg gtgggttcga agtccgatgc
     1381 gctcatgaga ccacctggtt ggcgaaaagg atgccgggat tcgacagcgg agggacggcc
     1441 tcagggccat gagaaatgcg agaaatctgc ggtgccgacg cttgccgtgg cgtcatcgag
     1501 agctggaggc gggttttcga taggtttgtc gtctcgagac ctttcatggt ttgccggata
     1561 ggggtcatga ctggccttcc tcgaggtacc gttggcaggc ttttaccgct ccccaggcag
     1621 tgccaatcgc cagacccgca ccgcttttcg tccgcatcca atcttgatgg gcgagaaggg
     1681 atcctgcaac aaataatcca gtaaacacag gcagttgtga agagtccacc ggacgaaaga
     1741 gcgcgtctac cctcacgccg gcccgatgga tgggatgccc ccgggtatcg aagaggtcgg
     1801 ctcgatgcca ggcttgtcgc gcatcgggtt gccatacggg caagttgaaa accgtctcgc
     1861 gaacgcctgt tctttccgcg gccaaacctc ctgccaaaaa acgcccggtg gctagcaaca
     1921 cggctggcgc acggatttct gtgagagcat ccgtttcacc cgcgaaaagc acgaaaccat
     1981 ccgggggctc ggcccgcacg cgcacgactt tttccgtcat gagtttggcc ccaagcccgt
     2041 gcagagcctt ttcaaaggca gcacgcaggc gcagacccgg cacgaaaccg ggcgtgcagg
     2101 gaatttcaaa gacaggtacg ccgagcctgt tttgcaggtg ttcgagtgcc cccagggaat
     2161 gttcgaagcc aaggaccgcg ggcaaaccaa cggcgatagc cccttgcaac aaaggcgtga
     2221 tccgttccgc aagacgatca cgaaaggggg cttcgtcgaa ttgacgagca agaaaggccg
     2281 gagagatttc gtgttgcgaa tccgttccgg gacattccac ggtcgaccag cgaagcaggg
     2341 gccaggccga acgaaggttc gaggccacga gttgcgcgga ataatccttc aatccgaaaa
     2401 aatcgatgag aagacaggag gatttgcgct gccaggcctg cacacccgct cccatcgtag
     2461 cagggaggca ataggtcgtt ttgcgggttc ccagggatgt gatcagctcc gaattggcct
     2521 cggaaacgcg aaaatacgaa taatttcgct gagatagaac gtccaggaga aaagaaaagg
     2581 cgtcgcgcag ggctggctcg cccacaagac cgtacggatg cgaaggctcg tttgccatca
     2641 gcgttttcaa ggcatcccac gggttatgga cccgtttttt ttcctcgagg ggatagacgc
     2701 ccagaaggtc gaaaaggccg gagggatgag cccaggagcc cgttggcccc aaaacgaccg
     2761 tggacagccc ccgggaggcg gcgaaaaccg cggcacaaag ccctgtcatg cccgcaccca
     2821 cgatggcaag gtcgaagtgc gcggtcatgg ctcgagttcc aatcccagca aagcgcaatg
     2881 aatcgcttca ttgagttccg cctggcgcgc ctgttcatga aaaagaaccg atcgttgccc
     2941 cttccagcgc tcgttcagga aggctttcaa ttccggaagc gtgccggagg cgtcagaagt
     3001 ccttgtcccc acgaggtggg caagcacccg cacactgcaa aaagagcctt gacaggcgcc
     3061 ttttcccacg cggctgcgaa gagcgacgtc caacaacgtg gggggcggca aggacggaag
     3121 tttttccaaa atatgcgtca tggcgctggg gggcaccatt tcgcattcac atagcagcca
     3181 atccttgggc ttcgccgcct ccgcccacag gcgcggtgaa cgtcccggaa acgtccataa
     3241 ggaagcatcg ggcaacgcta cctcagccgt ccggctcggc aggtcgcaac ccaggtgagc
     3301 gcagaccaaa tcccctgctt tttcggccat ttgccggaaa gtagtgagtt tgccccccgt
     3361 taccgtgatg aagttttcga ccccatgctc acggtgatcg aaaagggata ggtcgcggct
     3421 gacagagcgg tcatcgccgg agcagcgttg acgcaccagc ggacgcactc ccgcataagc
     3481 gcgcaagatt cgtgcttttt ccaaaagggg aacgagctga gccgtttcct ggacgatggt
     3541 atccacctcg gcaggggtcg gtgcgatgtc ctccagatcg tccacccgca acgaggtggt
     3601 tccgaggacg gagacggttc cgccgggaac gatgatatcg gcattggcgg gttttcgcag
     3661 tcgattgatg actctgtcgg tgaagcgacg ttgggtaatg agcagggtgc ctttggaata
     3721 gaggatttcc agggtgattc cggccatggc ggcgatttgt ccggaccagg cgccggcggc
     3781 gttgacgacc tcatcggcct cgatgacggt ttttatgccg gatcgggtat cgacaacgtg
     3841 ggttcgctgg attcttccgt ggcggcaatc gaatccaacg acgcgggttt gggggagaaa
     3901 tcgtgcaccc agatggcaag catgggccat attttcgagc gaaagcttga aaggatccac
     3961 ggtggcgtcg ggcacttcga acgctgcgat catggtcttg gacagagccg gttccatttc
     4021 ccaggcctgc cggatatcca cggggcgagc ggggatgcca cagcgggcac aaaggccggg
     4081 aaaatcagca atatagtttt catcatcgcc ttgcaccgcc gcaaaaaggc cccccgtgtc
     4141 ttcgaggcag tgcgaggcca gtcttcgcag gatctgtcct tcttcgtaac aatgccgtgc
     4201 gctttgcgcg tcgccggcca cgtagcgggc accgctatgc agcagtccat ggttggcgcc
     4261 ggaggctccc gcgttcagat ccgctttttc cacgacaacg gtgtgaatcc ctcggagggc
     4321 aaggtcgcgg gcaagccctg tgcccgtggc tccaccgcca atcaccaaaa cgttggctcg
     4381 aagagccgtc atgatacggg tttcatggtg gtcctgcccc atccggcgtg cagcctgtca
     4441 tgggccagtt gtatccttgt tcgacaggtc gattccgcgg caaggccagc ttttgtcaca
     4501 gtgcgtcata ttgcgataaa tgggctcggg agtcaagggc cgcgccccat gccgaaagcc
     4561 tgggcatggc atataacagg cgtaaaccag cgataagttg catcgtcgcg acaacgcgac
     4621 cggtaatagg taggtaaata tgcgtgcaga gggaatccga ggcgattcga acgcttggag
     4681 tggatgtttt cacgaggcga cggtatgggg ctcaagcgac gatcccaaag agaataccga
     4741 tatgaatcct caacgactcg acacatcccg ccgcataatc catggcgtca ggcagcgtgg
     4801 actggccatg gccatcgtgg gagggtgtac ccttttctcg atgatgggct gccggtcgaa
     4861 actgacctgc gaagcgattg caaagcgcaa cgcgacgtgt tcggaagcgt ttgtggaaga
     4921 ggcgaagcgc agaacacgag cgaaaatggc cgaacgtctt gcgtcgcttc ccgaggcgga
     4981 gcgaaaaaag atggtggcaa agatggaagc ttcgttcaag gaatcggcca aagaggttcg
     5041 cgccacggtc gagagcgatg agttcttgca gaactgtcgg gcgagttggc aagatccatc
     5101 gaaaatgccg aaggctttga aagaggagtt ggcccgatgc ctgtcgttgc cggactgcga
     5161 aggctatgcg acctgtttca tggattccgc ggcgttgtcg ccgtaaaagt cccccacgag
     5221 gagcttgtgg tcgaggcatc ccacggcacc tctcgccccg aagtgcccaa agagcatgtc
     5281 cgaccggctt tctgctgaca gatcccctca ttttttcctg aattcgaaag agatacgagc
     5341 tgaggactcc tgggtcttga tttggctggg tccttgcccc ccttagggca cccccaacag
     5401 ttgccccggg ctactaaaca ttggactggc gttctgcggt gaacgatatg ggattatcgc
     5461 gaccgttttt gtgcgccgtg cgccgtgacc gtgaccgtga ccgtgaccgg tttgcgtggg
     5521 ccgggaccgt ggccttgacc gtgaccgttt tttgtaccgt gcgccgtaac cgtaactgtg
     5581 accgtgaagg aaaagctcgg aaaaaatgag ggggtggctc agggcccttt gaccgcggcc
     5641 cgatggctcc caccatgtct ccatacgaac ataaataatt gaaattacat attatttttg
     5701 aaatatgagt ccatggtcaa gccttccact tgagaaatta gacaataaaa cggatatttt
     5761 cgcgttgcag gcgaaagccg gacaggg
//
LOCUS       DAOSOR010000378         5773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14055_length_5773_cov_3.888772, whole genome shotgun sequence.
ACCESSION   DAOSOR010000378 DAOSOR010000000
VERSION     DAOSOR010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14055_length_5773_cov_3.888772"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..1224)
                     /locus_tag="PK710_17595"
     CDS             complement(37..1224)
                     /locus_tag="PK710_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11596.1"
                     /translation="MNRFLPIGLSLLLITLLSCGEEESSPAQVQATHDSGTGSTDSSD
                     DSMLLDTSDDQAPDRDITTPDADIPLDDIPSYIVEATSGEWTAIGTNAMTDVIFDYGS
                     AATPDGFRSNLQGIMDAWSGGTYASPHHRLYVNGGGHGDYDGNEWYAFDVSHQRWERI
                     NDPSLYRPGTSADGIYPDGAPEPIHTYNNLVYAPTTNKLYRAGKSGSPLSNLWAFDLN
                     TKKWEKTPVPANDPHGNVARWDPTTNRVFLCRNSSQASGYTYIVWFDPITSTLTDTGA
                     ATRWYSGTGALDTTHGEFVYTGGKQAPIRVNTTTYEITNVDVTGDTEIQNVPAPGFVY
                     DSRRDRFTAWGAAGDTRTLYHISRKDWKWTAETPTTGDTPGAPNANGIYGRFQYIPHG
                     GVL"
     gene            1391..1546
                     /locus_tag="PK710_17600"
     CDS             1391..1546
                     /locus_tag="PK710_17600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11597.1"
                     /translation="MASVMDGASWANAAFAKRAMVRWMSKTGASHDLAPLIRRDGAVV
                     EVLRCWV"
     gene            1825..3255
                     /locus_tag="PK710_17605"
     CDS             1825..3255
                     /locus_tag="PK710_17605"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT11598.1"
                     /translation="MARDSTTLTTSHFRSDSIVEPMYVPALVLVWSAMQPWRVGEVAL
                     LDPQRGPFLLGRGDSSEPPIAEFAWQRPTGLDRQPPLAGPRISRRQLLLFPHKNQSLG
                     VERIGRCPMIVGGNRVDKAELMPGDILELEEQLTFWVTSRPSMLPALDALPEHAVPCF
                     GAADPYGIVGESPVIWTLREQLAFAAVANQHVLLLGESGSGKELGALAIHALSSRASK
                     AMISRNAATLPSGIIDAELFGNVRDYPNPGMRERPGIIGEADGSTLFLDEIGELPHDL
                     QAHLLRVLDAGGEYQRLGDARPRRAHLRIVAATNRDPDQLKHDLLARLTLRIRIPGLD
                     ERREDIPLLVRRLVKQAMRASAMVGQRFVQHFEGGHEEPRITQSLMTRMLRLAYSHHV
                     RDLEQVLWRAMSTSRRHRIEDTPEVQEAYPVASPIVTLEQPELTPQQIRQALDAHGGN
                     VTHTARALGLSSRFALYRLMKKWGLE"
     gene            complement(3266..4948)
                     /locus_tag="PK710_17610"
     CDS             complement(3266..4948)
                     /locus_tag="PK710_17610"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT11599.1"
                     /translation="MNEPRVSRIPKGVLLGSAYRVEEWLGGGGYGEVYRAHSLRMDRP
                     VAIKVMKPQASRVTRERFAREAQLTKRLEHPNTVRLLDFGGLDDDQPFLVWELLRGET
                     LAERLERKGPMPPEEVANMARQVLSSLEEAHGLGIIHRDIKPSNLMLCDFEGEKDFVK
                     VLDFGVAKDVLTALNDLTEEGEVVGTPRYMAPEQVLGKQVVPATDLYALGLVMAEALT
                     GSPMVKASSKTQAVAFQAMETDLPIDPQVLEGPLGRAIRQATRKRLGARFGSSAAMRD
                     TILEEMAAANGPGPFTMKENASHHPCSEPVTLEPVLVSESTSCITAVGTETKKGKTRS
                     RLSVGLGVVVVASLGAGILWLFNPSSPKDMPSNPTVQDTPRAVPSNAFSMQMERDWHP
                     KSEPLSGAITLSAFDPLAFLPEAQVRAQKEFADAALTNIQVIGVWQHSIVNLLAVPNG
                     SATYWFRSQQASTPPASFPSNIAFETRCSVAITVDQRGLSAYMPISTCDEPFVPSPRC
                     GLRNVIERFASTTGTPARHFGFQAYYRSKQGTATWLVEAGSYGSEYLTDDCP"
     gene            5264..>5773
                     /locus_tag="PK710_17615"
     CDS             5264..>5773
                     /locus_tag="PK710_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11600.1"
                     /translation="MKSIAFVSSTLVFFLAAMVGCSSTNDDESNPNGTGGSGGTGLSD
                     AGPDTSAGGESGVGGTGGSGATGGSGGAGPEDAGPDATKPLPGSGCPSTTGNKIKGVV
                     RLSTLHATPEPTGPSVVVKLPCGREVKDPSKATCGKAPLHVFLCSTADCSAAADPIRS
                     YLESDASLVQ"
BASE COUNT         1186 a         1575 c         1672 g         1340 t
ORIGIN      
        1 accgcaggca gttgcgcgaa agcgacaatg tatacgttaa aggacgcccc catgggggat
       61 atactgaaaa cgcccgtaaa tgccattagc gttgggcgcg ccaggcgtat ctcccgtggt
      121 aggtgtctca gcggtccact tccagtcctt acgggagata tggtacaagg ttctcgtgtc
      181 gccagcggct ccccatgccg tgaatcggtc tcgacgggaa tcatacacga accctggagc
      241 tggcacgttt tggatttccg tgtctcctgt gacatccacg tttgtgatct cgtaagttgt
      301 tgtatttact cggataggcg cctgtttgcc gcccgtataa acgaattccc cgtgcgtggt
      361 atccaaggct cccgttccgc tataccacct ggtggcggct ccagtatccg ttagcgtgga
      421 cgtgatggga tcgaaccaca cgatatacgt gtagccgctc gcttgagacg aattcctgca
      481 caaaaatacc cgatttgttg tgggatccca tcgggcgaca ttgccatgcg gatcgttcgc
      541 gggaactggc gttttttccc atttttttgt attcaagtcg aaagcccaga ggttcgacag
      601 aggagagccg ctttttccag cacgatatag cttgttcgtg gtgggcgcgt agacaagatt
      661 gttgtaggta tgaatcggct cgggagcccc atccggatag attccgtccg cgctggtccc
      721 aggtctgtat aggctcggat cgtttattcg ctcccagcgc tggtgagaga cgtcgaaggc
      781 ataccattca tttccatcgt aatccccatg accgccgcca ttgacataca gacgatgatg
      841 tggggatgca taggtgccgc cgctccatgc atccatgatt ccctgaagat tcgaacgaaa
      901 tccatccggg gttgccgcgg agccataatc gaatatgacg tctgtcatcg cattcgttcc
      961 aatggcagtc cattcgcccg atgtggcttc gacaatgtag cttgggatgt catccagcgg
     1021 tatatcggca tccggtgtcg tgatatcacg gtcaggggct tgatcgtcgg aggtgtccag
     1081 caacatggag tcatcggatg aatccgtcga gcccgtgccc gagtcgtggg tggcctgaac
     1141 ctgtgcaggg gaggactcct cttccccaca ggaaagtagc gtgatcaaaa gtagactcag
     1201 cccaatgggt aggaaacggt tcatggtggt tttctcctac cgggaactct gcataattca
     1261 tgcccgtcca tgtatttgta gcttgttgta aatattagtt aaaatattat gtgctgcgtc
     1321 ggttctgtcg cacgaacctt ccgttcaaag catgaggagg gggagcccag catctttttg
     1381 ccggtctctc atggcttcgg tgatggacgg cgcttcatgg gcgaacgcgg cgtttgcaaa
     1441 acgagccatg gttcggtgga tgtcgaaaac aggggcaagc catgacctcg ctccgttgat
     1501 tcgtcgcgat ggggcggtag tggaggtcct tcgttgctgg gtttgagacg tggcctcgca
     1561 aaggtgttgc ttgtcgtacc ggtatttggg gcttaggaag cggagccata cggaaagacg
     1621 gcaatggcta ctaaggagtc tcgcagccat aagctcctcg ttgccgttgg ggtattcccg
     1681 tgaaaaatga gtcatccaaa cgcctgacgc gtcttcgacc ctttcgagcc catcgctttg
     1741 atcatcgcat cgactcgcaa aacccggttg ccactgcccc agccatcggt acaatcagcc
     1801 gatcggttgc accaaaattc gcccatggct cgtgattcca caacgttgac gacctcccat
     1861 ttccgctctg attcgatcgt cgagcctatg tacgtccctg cgctcgtgct cgtatggtcc
     1921 gccatgcagc cttggcgggt gggtgaagtc gcattgctcg atccgcagcg cggaccgttc
     1981 cttttgggcc gtggcgattc ttccgagccg cccatcgccg aatttgcctg gcagcgaccg
     2041 acgggactcg ataggcaacc gcctttggca ggaccgcgga tttctcgacg tcaattgctc
     2101 ctatttcctc ataaaaatca atctcttggc gtcgaaagga tcggtcgatg cccgatgatc
     2161 gttggcggaa accgggtgga caaggccgag cttatgccgg gcgatattct cgaactcgag
     2221 gagcaattga cgttttgggt cacctcgcgg ccttcgatgc tcccagccct cgacgctctt
     2281 cccgagcatg cggtgccttg ttttggggct gcggatccct acggcatcgt gggtgagtct
     2341 cctgtcatct ggaccttgcg agaacagctt gcctttgcag cggtagccaa tcagcatgtg
     2401 ttgttgctgg gagaaagtgg ttcgggcaag gaactgggag cccttgccat tcatgccctg
     2461 tcctcgcgtg cttccaaggc catgatttcc cgcaatgccg ctaccttgcc ttcgggcatc
     2521 atcgatgcgg agcttttcgg taacgtgcgt gattacccca acccggggat gcgcgaacgc
     2581 ccgggcatca tcggggaagc cgatggttct actctatttc tcgacgaaat cggggaattg
     2641 ccccacgatc tgcaagccca ccttttgcgc gtcctcgacg caggcggtga gtatcaacgg
     2701 ttgggagatg ccaggccccg acgggctcat ttgcggatcg ttgcggcgac caatcgcgat
     2761 ccggatcaat tgaaacacga tttgctagcg cgtttgacgt tgcggatacg tattcccggc
     2821 cttgacgaaa gacgggaaga cattccgctt ttggtgcgac gtcttgtgaa acaagcgatg
     2881 cgtgcgtcgg ccatggtggg gcaacgattt gtgcaacact tcgaaggcgg gcatgaagaa
     2941 ccgcgaatca cacagtcgtt gatgaccagg atgctacgtc ttgcctattc tcatcacgtt
     3001 cgagatctcg aacaggtcct gtggcgagcc atgtccacga gccgtcgtca ccggattgag
     3061 gataccccgg aggttcagga ggcgtatccc gtggcatcgc ccatcgtaac cctggaacaa
     3121 ccggaactga caccgcaaca gattcggcag gcgctcgatg cccatggtgg aaatgtcacc
     3181 catacagctc gcgctctggg gctatcgagc cggtttgcgc tttatcgact catgaaaaaa
     3241 tgggggctgg aatagtaccg cggtttcaag ggcaatcgtc ggtcaaatac tcggagccgt
     3301 acgaaccagc ctccacgagc cacgtggccg tgccctgctt cgagcgataa tatgcctgaa
     3361 atccgaagtg tcgagccggt gtccccgtgg tggaagcaaa acgttcgatg acgtttctga
     3421 gcccacaacg cggcgacggt acgaaaggct cgtcacaggt cgaaataggc atataagcgg
     3481 acaatcctcg ctggtccacg gtaatcgcga ccgagcatcg ggtttcgaaa gcgatattgg
     3541 aaggaaacga cgcaggaggg gtagatgctt gctgcgagcg aaaccaatag gtggcagagc
     3601 cgttaggcac ggcgagcaag tttacgatcg aatgttgcca gacccctatc acttggatat
     3661 tggtcaaggc agcatcggcg aattctttct gggctcggac ctgcgcttcc ggcaaaaaag
     3721 caagcggatc gaaagcggat agggtgatgg cacccgaaag aggttccgac tttgggtgcc
     3781 aatctcgttc catctgcatg gaaaatgcat tggatggtac cgctcggggg gtgtcctgaa
     3841 cggtcggatt ggagggcatg tctttggggc tggagggatt gaatagccac aatatccctg
     3901 cgcctagcga ggccacgacc accacgccca gccccaccga gagccgagaa cgtgttttgc
     3961 cctttttcgt ttccgttccc acagccgtta tgcaagaggt ggattcggaa acgaggacgg
     4021 gttcgagagt cacgggttcc gagcacggat ggtgggatgc gttttccttc atcgtgaagg
     4081 ggcctggacc gttcgcagcg gccatttcct cgaggatcgt gtcccgcatg gcagccgaag
     4141 agccgaaacg tgctcccaat cgtttgcgtg tggcttgtcg gatggctctg cccaacggtc
     4201 cttccagtac ctgggggtcg ataggaagat cggtttccat ggcttgaaaa gccacggcct
     4261 gggttttgct cgaagctttc accatgggtg agcctgtcaa tgcctccgcc atcacgagtc
     4321 caagagcata aaggtcagtg gcgggaacga cttgtttgcc tagaacttgc tcgggggcca
     4381 tataacgagg agtccccacg acctctcctt cctcggtcag gtcgttcaac gcggtgagta
     4441 cgtctttggc gaccccgaaa tcgaggacct tgacgaaatc cttttctccc tcgaaatcac
     4501 aaagcatgag attggagggt ttgatgtctc ggtggatgat gcccagacca tgagcttctt
     4561 caagagaaga caggacttgt ctggccatgt ttgccacctc ctccgggggc atgggcccct
     4621 ttcgttctag ccgttcggca agggtttctc cgcgcaacaa ttcccaaacg aggaaaggct
     4681 ggtcgtcgtc cagtccgcca aagtcgagca aacgtaccgt attggggtgt tccaggcgtt
     4741 tggtaagctg tgcttcccgt gcgaaccgtt ctcgcgttac cctgcttgcc tggggtttca
     4801 tcactttgat ggccaccgga cggtccatgc gtaacgaatg cgcacggtat acctcgccat
     4861 agccgcctcc gccaagccat tcttctactc ggtaggcgct gcccaacaac acccctttgg
     4921 ggattcgtga gactcgaggc tcgttcatac ttgcagtcct tgcatcatga ttgattttcc
     4981 ctcgaaggca atgtcgaagt caaaccatga ccttttccca tggtgctacg tagatcggcc
     5041 tttgattctt ccttggtccg ggtgcggtgg cttttatcgg gtagcgagca agcgtgcgcg
     5101 aacgtgcgag cacgcgtgcg catgggggaa cgcacagggg agtcgtgcta gaaaagccca
     5161 tatttactca tatttcttgc caaaacacga ttggcatact aatagctatg aattgggcga
     5221 ctcatcaaaa tgggatcctt caaccagact gcggagtatg attatgaaat ccattgcctt
     5281 tgtctccagc acgcttgttt ttttcctggc ggccatggtc ggctgctcct cgacgaatga
     5341 tgacgagtcg aatccgaatg ggacgggagg ctcgggggga acaggtctgt cggatgcagg
     5401 cccagacacc agcgctggcg gagaaagtgg agtgggcggt actggtggat ctggcgcaac
     5461 gggtgggtct ggaggtgccg gacccgagga cgccggaccg gatgccacca aacccttacc
     5521 gggttcagga tgtccctcca ccacgggaaa taaaatcaaa ggcgtcgttc gcctctccac
     5581 cttgcacgca actcctgaac ccaccggccc gagcgtggtc gtcaaactcc cctgcggtag
     5641 agaggtcaag gacccgtcga aagccacctg tggcaaagcg cctttgcacg ttttcctgtg
     5701 ctcgaccgcg gattgttcgg cagccgcgga cccgattcgg agttatctgg aatccgatgc
     5761 ctctctcgtt cag
//
LOCUS       DAOSOR010000379         5771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14060_length_5771_cov_3.796886, whole genome shotgun sequence.
ACCESSION   DAOSOR010000379 DAOSOR010000000
VERSION     DAOSOR010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14060_length_5771_cov_3.796886"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..2361
                     /locus_tag="PK710_17620"
     CDS             211..2361
                     /locus_tag="PK710_17620"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOT11601.1"
                     /translation="MKRSSAPYRIAIAATLFAHSAHPQSVVFAPTGTQADLQAAIEAV
                     ESPAVIVIPPGKWQVVGSINLTKDAITIIGAGMDKTRLHRDAPPPNADVATLTQYRAP
                     FFRSKATSSVRLSSFGITSWQAASLEDREVGISFSNVSDFRVDQCSFRFTGNSGVMVG
                     GESRGVINNCTFRDIYEASIGNYGYGDSVYGTVHTGEPYGTERATFIEDSLFEGCRHA
                     VASNRGARYVFRHNTVKHNTVSHAIDTHGAEYPPSPKPTCNPCYDPDPDNPGTEWAIV
                     HDNVIDDPDYSGSAITLRGGKGLVYQNTIRGYTRAIQLSKQTPQATGPIHIWDNDIID
                     PTQLVTGVGTCCGSGPTWELTKPSTYNPYPYPHPLTDHLVAHAGPDQRVMPPPQSKVA
                     QVFVDASASKAKKGNIQGFGWFTSPQPSSSCSRDLLELPIGSHVVLLAVRGPTGSIEV
                     DTFTVDVLPPGPLASSSQWNNLFFPPFPKQGTIVFDLVAHSNNLDAYVGLTGRIPVQS
                     HGDHAMQLRLNSQGTFDVRNAEVYEALAPIPYESGKTYHIAIDFDVATRRYDVSVDGK
                     PIATQFSFRTPQALIGQITAWHVDGSAGVEIKDLAYSGQQLGPDPACQDPVDAGVDAT
                     IPDAQLPSDAERDVAKDGVSDHAVEATTPMPNDAATPPSADSQEGCGCRVPQSHNQPY
                     SLWLIVFGALCGCRGLRRNTHRKTDDHGWFEPTP"
     gene            2503..3513
                     /locus_tag="PK710_17625"
     CDS             2503..3513
                     /locus_tag="PK710_17625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11602.1"
                     /translation="MPWFSTNPGRGDSNRQHVCGAFTPLWSVHSATFSVEKRNATKNE
                     FIFAGTTIARHWGMKTLIPFVCLTSMLSFSACSIDLESEPEGPTAKDAQQAWPTKPTV
                     EPISLESITRACIAYTACDADNALGLMQVEICLTDLAWSAERAIPISTSNLLFSVLGD
                     TNERAEFFVDCVEKSKDCGEIKGCITSREADFSCEEDGCKANTTYSVSCDGTVAVMTT
                     DNSTIRRDCARVFGECDSKSPTGCTDRHYTASPSSPPQPDRCDGDVRLGCDSLQQVSY
                     RDCSRLNGVCKQTEDSAICHYGDAICEKTVRCEGETLHACLDGASATVQAPHLCPPPD
                     QP"
     gene            complement(3517..4866)
                     /locus_tag="PK710_17630"
     CDS             complement(3517..4866)
                     /locus_tag="PK710_17630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11603.1"
                     /translation="MSPRAHAQDSSSSVAQEVRIVILPTLTPTGEVPTDKALGRPELQ
                     NGLLWTKARELDNLLHDTAQDLGFVVDLSEPRTSDVIVSERELLARASRSVSWQLSPR
                     LEFEGSDIVLRLLLVPPRGEHVLVRTETVSSEDFQVRAVVMFRDLVRGARPSYDDRGS
                     GFEGDLRPMRPEQRRSEGRAVLVMHAAALGGFVGYSLQKSSGSDDARLTYPLMALGTG
                     VGFGGALIIAEEWDVGLGDAWYMSAGAVWPVFGGMMLAKGRDVQPELDRYAWAVGSGL
                     AGVSLASISLSFAGMGTGGAAVAHSGGFIGTGLGAGVEMAIRGKADVTPYEGLGYGAL
                     GGVAVGGIWGRWVQESPSRIMMVDVGAGLGALSFAAVGSPLLIGDSTEGRQRAWVLVT
                     MSGAVAGGLAAFWMTAASEEKSADAPLTHPMAGVIGQSLRADGTYAPVMGVGWSGLW"
     gene            complement(4917..>5771)
                     /locus_tag="PK710_17635"
     CDS             complement(4917..>5771)
                     /locus_tag="PK710_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11604.1"
                     /translation="GTAGNYAYTDHTGQTYQLEGTKAGERSAGVSRGTTGIEVHRLVS
                     RRIKYLEPYGGFRALFEFPTDKSDYGLMDLEGSLVNHPPLEGWMVMGMMVIPWENREQ
                     FQRLTFDARVEGAYRSEGRDYSELFDALGSSDAMSLRRPNYASYQRNPDPATAQTYPS
                     VVNPSSQKVYFTGLTDVQAHGKFRASGSVTWQAGEYVKFQLGVGYMYVQPHFITMDQP
                     CNPDFKKDMSKAGPCRNVSRVGDTSTFSATGIPNPNYRPSINQVGQRFKVDDVSVVDL
                     WLNGVVMF"
BASE COUNT         1323 a         1927 c         1411 g         1110 t
ORIGIN      
        1 agtaaaacca gtgaaatatg aggggatctt tcagcatgat gaggatacga atggggaaat
       61 tggccgtgcc gccatgtcat atcgcctgcc cgcgccatac gataggccat cccttatggc
      121 attgtcgttt cggttctctg cgtccgcatg caaacccgga ctgcctcgcg tgcgtggata
      181 cccgcccgcc ccgtcgagta cactacccac atgaaacgct catcggctcc ctatcgcatc
      241 gccatcgcag cgacgttgtt cgctcactcc gcccaccccc aatccgtggt ctttgctccc
      301 acgggaacac aagccgattt gcaagccgcc atcgaagccg tcgaatcacc ggccgtgatc
      361 gtgatccccc ctggcaaatg gcaagtcgtg gggagcatca atctcacgaa ggacgccatc
      421 accatcattg gcgcggggat ggataaaacc agattgcata gagatgcccc tccccccaac
      481 gccgacgtcg ccacgctcac ccaataccga gcccctttct tccgctcgaa agccaccagt
      541 tcggttcgtc tttcttcgtt tgggatcacg agttggcagg ccgccagcct cgaagaccga
      601 gaggtcggca tttctttttc gaatgtatcg gactttcgcg tggatcaatg ctccttccgt
      661 ttcacgggaa atagcggcgt catggttggg ggcgagtccc ggggcgtaat caacaattgt
      721 acatttcggg atatatacga agccagcatc ggcaactatg gctatgggga ttccgtctat
      781 ggaaccgttc atacgggaga accttacggc accgagcgcg ccaccttcat cgaagacagc
      841 ctcttcgaag gctgcagaca cgcggtcgcc tcgaaccgtg gcgcgcgtta cgtattccgg
      901 cacaacaccg tgaaacacaa caccgtgtcc cacgccatcg atacccacgg cgctgaatac
      961 cctcccagtc ccaaacccac ctgtaaccca tgctatgatc cggatccgga taatccggga
     1021 accgaatggg ccatcgttca cgacaatgtc attgacgacc ccgactacag cggctcggcc
     1081 attaccctgc gcggcggcaa gggactcgtc tatcaaaata cgatccgcgg ctacaccagg
     1141 gccattcaac tctcgaaaca gacgccacag gccacgggac ccattcatat ctgggacaac
     1201 gacatcatcg atcccacgca attggtcacc ggcgttggaa catgctgcgg aagtggaccg
     1261 acttgggagc tgaccaaacc gagtacgtac aatccctatc cgtatcctca ccctctcacg
     1321 gaccatctgg tcgctcacgc tggaccggat caacgtgtca tgcctccccc ccagtccaag
     1381 gtcgctcagg tattcgtcga tgcctccgcc tccaaagcaa aaaaaggcaa tattcaaggc
     1441 tttggttggt tcacctctcc tcaaccgagt tcatcgtgct cccgcgactt gctcgaactc
     1501 cccatcggat cccatgtcgt cttgctcgcg gtccgcggcc caaccggttc catcgaagtg
     1561 gatactttca cggtcgacgt cctgcctccg ggcccgctcg catcctcgag tcaatggaac
     1621 aaccttttct ttccgccgtt tccaaaacag ggaaccatcg ttttcgatct ggttgctcat
     1681 agcaacaacc tcgatgccta cgttgggctc acaggacgaa tccctgtcca atcccacggg
     1741 gaccacgcca tgcaacttcg gctcaatagc caaggaacct tcgacgttcg aaacgcggag
     1801 gtctacgaag ccctcgctcc catcccctac gaaagcggaa aaacttatca tattgcgatc
     1861 gacttcgatg tcgccacacg gcgttacgat gtgagcgtgg atggtaagcc catcgccaca
     1921 caattctcat ttcgaactcc acaagccctg attggacaga tcacggcttg gcatgtcgat
     1981 ggttcggccg gggtcgaaat caaggatctg gcttactcgg gacaacaatt gggccccgat
     2041 cccgcttgcc aggaccccgt ggatgcgggc gtggacgcca cgattcccga tgcacaacta
     2101 ccatccgacg ccgaacgaga cgtggccaag gatggtgttt cggatcatgc ggtcgaagcc
     2161 accacaccca tgcccaacga tgcggcaacc ccgccaagcg ccgatagcca ggagggctgt
     2221 ggttgtcgcg tgccccaatc tcataaccaa ccgtattcat tatggttaat cgtgtttggc
     2281 gccttgtgcg ggtgccgggg cttacgccgg aacacccatc gcaaaacaga cgatcatgga
     2341 tggttcgaac caaccccatg aactcacctc agccgaccgg atcgccgtaa agggcatgct
     2401 cgtaaacgtg ctcgcaacta tccagatacg gatatgcatc ggggcaatga tcggatgcct
     2461 tggcatggtt gccatgcggc agacagaaca aagagtcggg tcatgccatg gttcagcacg
     2521 aatcccggac gcggtgactc caaccggcaa cacgtctgtg gcgccttcac accactgtgg
     2581 tctgtccaca gcgccacttt ttccgttgaa aagcgcaatg ccacgaaaaa cgagttcatc
     2641 ttcgctggca caaccattgc tcgtcattgg ggcatgaaga cgctcatccc tttcgtttgc
     2701 ctcaccagta tgttgtcgtt ttccgcctgt tccatcgatt tggaaagcga accggagggc
     2761 cccacggcca aagatgcgca gcaggcttgg cctaccaagc ccaccgtaga acccatcagt
     2821 ctcgagtcga taacccgtgc gtgcattgcc tacaccgcat gcgatgcgga caatgccctc
     2881 ggcctcatgc aagtagaaat ctgtttgaca gacctggctt ggtccgctga acgagccatc
     2941 cccatctcca cgtcaaacct tttgttttcc gtgttgggtg acaccaacga acgagcagaa
     3001 ttcttcgtgg attgcgtaga aaaatccaag gattgcggcg aaatcaaagg atgcatcacc
     3061 tcacgggaag cggacttctc atgcgaagaa gatggctgca aagccaatac aacatactcc
     3121 gtgtcctgcg atggaaccgt cgccgtcatg acaaccgaca actccaccat ccgtcgtgat
     3181 tgcgcccgcg tcttcggtga atgtgactcg aaaagcccca cgggttgcac cgaccgacac
     3241 tacaccgcgt ctccctcctc tcctcctcag cccgaccgtt gcgatggcga tgtccgtctc
     3301 ggttgtgaca gtctccaaca agtcagctac cgcgattgct cgaggctgaa cggcgtgtgc
     3361 aagcaaaccg aggattccgc catctgccac tacggcgacg ccatctgtga gaaaacagtg
     3421 cgatgcgaag gagaaacgtt acacgcttgt ctcgatggag catcagctac cgtccaagcc
     3481 ccccacttgt gtccgccccc ggaccaacca taagccttac caaagaccac tccacccaac
     3541 gcccatcacc ggagcatacg taccgtccgc tcgtaagctt tgtccgatca cccccgccat
     3601 gggatgagtc aaaggcgcgt cggcgctttt ttcttcactc gccgctgtca tccaaaacgc
     3661 tgccaatccc cccgccacag cgccagacat cgtcaccaac acccacgcac gttgccgccc
     3721 ttcggtcgaa tcaccgatga gaagcgggct tcccacagcc gcaaaagaca gcgccccaag
     3781 cccagcaccc acgtccacca tcatgatgcg cgaaggcgat tcctgcaccc aacgtcccca
     3841 tattccaccg accgcaacgc cccccagagc accgtatcca aggccttcgt agggagtcac
     3901 atcggccttg ccacgaatcg ccatctccac gccagccccc aaacccgtac cgataaatcc
     3961 accagaatgc gccaccgccg cgccacctgt gcccatgccg gcaaagctca gggatatcga
     4021 cgccaacgac acgccggcca ggccacttcc cacggcccac gcatagcgat ccaattcagg
     4081 ctgcacgtcg cgccccttcg ccaacatcat tcccccgaag accggccaaa ccgcccccgc
     4141 ggacatatac cacgcgtccc caaggcccac gtcccactcc tccgcaatga tcagcgcacc
     4201 cccaaaaccc acgcccgttc caagcgccat caacggatac gtcagcctcg catcgtccga
     4261 tccactcgat ttttgaagag aatatccaac gaatccgccc agcgccgccg catgcatcac
     4321 aagcaccgcc cttccctcgg agcgacgttg ttccggtctc atcggacgta gatctccttc
     4381 gaatccagaa ccccgatcgt cgtaggaagg ccttgcccca cgcacaaggt cgcgaaacat
     4441 cacgacggct cgaacctgga agtcctccga tgataccgtc tcggtgcgaa ccaaaacgtg
     4501 ctcccctctc ggtggcacca aaagcaaacg aaggacgata tccgaacctt cgaattcgag
     4561 gcgtggtgat aactgccacg aaacggaacg ggaagcgcgg gcgagcaatt cccgctccga
     4621 cacaatgaca tcggaggtgc gaggctccga caagtcgacg acaaacccaa ggtcttgcgc
     4681 cgtgtcgtgc agcagattgt ccaactcccg cgccttggtc caaagaagac cattttgcaa
     4741 ttcgggacgc cccagggcct tatccgtagg cacttcaccc gtgggcgtca gggtcggaag
     4801 aatgacaatc ctgacctctt gcgcaacgga agaggatgag tcctgggcgt gagcgcgcgg
     4861 cgacacgccc gccacaaagc acgcaaggcc gagccatgat gcttttgcga aacgcctcag
     4921 aacatcacaa cgccattgag ccacaggtcc accacgctga catcgtcaac cttgaaccgt
     4981 tgcccaacct ggttgatcga cggtcgatag ttgggattcg gaataccggt cgcgctgaac
     5041 gtggaagtat cccccacccg agacacgttt cggcaagggc ccgccttcga catgtctttt
     5101 ttgaagtccg ggttacacgg ctggtccatc gtgatgaagt gcggctgcac atacatatat
     5161 cccacgccga gttggaactt cacatactcg cccgcctgcc acgtcacgct acccgaagcc
     5221 cgaaacttgc catgggcctg cacatccgta agccctgtga agtacacctt ctgcgatgat
     5281 ggattgacca cggaaggata ggtttgcgcc gtcgcaggat cgggattgcg ctggtacgaa
     5341 gcgtagttgg gacgacgcaa agacatcgcg tcgctggagc ccagggcatc aaaaagctca
     5401 ctataatcgc ggccttccga acgataggcc ccttcgacgc gcgcatcgaa ggtgagccgt
     5461 tggaactgct cccgattctc ccaaggaatg accatcatcc ccatgaccat ccatccttcc
     5521 aacggcggat ggttgaccaa cgatccctcc agatccatca aaccataatc gctcttgtcc
     5581 gtgggaaact cgaatagtgc ccggaaacca ccgtacggct ccagatactt gatacgtcgc
     5641 gagaccaacc tatgcacttc gatacctgtg gtgccacgac tcacccccgc ggaccgctcc
     5701 cccgctttcg ttccctcgag ttgatacgtc tgcccagtat gatccgtata cgcgtaatta
     5761 cccgccgtcc c
//
LOCUS       DAOSOR010000380         5767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14068_length_5767_cov_4.834034, whole genome shotgun sequence.
ACCESSION   DAOSOR010000380 DAOSOR010000000
VERSION     DAOSOR010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14068_length_5767_cov_4.834034"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..2078
                     /locus_tag="PK710_17640"
     CDS             27..2078
                     /locus_tag="PK710_17640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11605.1"
                     /translation="MRLRSAGWSIAVTISLLGVQSPAKAGDFDARGVFSFASDAVVKE
                     SFEAFSPIEAGGSKAEPVDSQTALDGQKILRLTLANEGLRLAVDLVSGFGVVRLSYWM
                     RGDAVGGLAVDSDERPSNLAQAYPTGRVTSDGWMEMRTAPIAIDGQAKGLDARVFFAG
                     YDGSETRTLDVDAVEIERLGDAVEPPSCQGIDLQGACGPEQLCLAGRCHDAAVWFPPL
                     PVEENRDRLIDYWKQKIHDTYGPILPRKTAMPDALHTLEAARNASSNVVFWSRFAESI
                     RRLRDAHTYTRSPSLSQLSFGPPLNVCFFEGIGDRSQAAWPSDPSFGDILVSHTGTSH
                     TWGLRQGDRLVAVDGIHPVAWVRSLMAHSLWTWEADEPRQVANTVSLLRQLIPLHART
                     VTVVHCDAMAKSCDDKATVLTVAQMEPASPALEPISCDNRPFYHVPSAPENHDFGDSL
                     ADESVVLEDWLDTSSPEEGLRGIVWNSLLGGWPGSKLDAQLRQATTTWKASARGVLMD
                     HRQGHGGTMVTADILISFSRQGAVPLASLFRNRAADEGPQTLEEGKELFLKIRGYAGI
                     HVGSNAPQVHIPVAFLTTWDVSASDFLPYLMKGASQVRLFGPGPTMGAFGTFFQYSYW
                     GGLRWSLGAEDSLTPEGITLCGRGVDPDEVVFPKQSDLLEGKDTVHEAAVHWLRKEIE
                     P"
     gene            2075..2941
                     /locus_tag="PK710_17645"
     CDS             2075..2941
                     /locus_tag="PK710_17645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11606.1"
                     /translation="MKRIGMPSVLLWTAMITVGCDSAAPDVNPAPRADAGTQKEASVD
                     TGLDVVEAQAPDVEQDVVDDGGPMIRQVYQRDPFGRLDPTNLLHDGDFELSAADSMQY
                     PWLGLTASNYVTGVDCRSGLRCIELSPSEYVAGVFVWPETAAVEVSFHGKLRSSQDCE
                     KEAVGVVMMADAYGGELMIPAKTSEPVDGWCQYAAELSVPSNVGYHLWVLLLAARQQA
                     TGAVVFDDVTMVASSFRTGRQALSVPSQDDAWLKQARKKIQGWLPPNPPRKPLPIRNI
                     TAKKKLSPNAGI"
     gene            complement(2978..4123)
                     /locus_tag="PK710_17650"
     CDS             complement(2978..4123)
                     /locus_tag="PK710_17650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11607.1"
                     /translation="MGKFRFLLSILAIPLSILACSSDEDSSNQAPATGGTGGATTDAT
                     TDVEADTTLDAPSDSIADQQAEAPADVATQDVVEASDAPQETDIDADPDAQVDRPCTL
                     LGQECEPGEKCSPEGKDDALVCRPDGDKSLGNACGENGFDDCLSGLACVEYSSKLSTC
                     RPLCIDTASCPQPTAQSCEPWFGPAGAVAGVCLGDLCSPPSTGCKADERCTVLSVASR
                     AVTACVPAGAVKVGGDCTVDECEPGAMCVHAGGKYVCRSFCHGGNDCNDDDLHCVWPW
                     GDDVAFGLCRSGCDPVRQKGCDDNEGCYYMDPETGSTDCWLAGGLLPGADCSNMSLCQ
                     PGFDCVLEPGSSPFKYYCRAYCDDYTPCAAGTCTTTQATAALKFCMP"
     gene            complement(4352..4645)
                     /locus_tag="PK710_17655"
     CDS             complement(4352..4645)
                     /locus_tag="PK710_17655"
                     /inference="COORDINATES: protein motif:HMM:NF021188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HOT11608.1"
                     /translation="MTSVHVLHPHRLPLREARNRVERAAIRAAEKYRLVWRWADNVLE
                     VLPPPGVAKGAKARLSLREGAALAEVDIPWSYRLLQDRIASRLADALRDLLAC"
     gene            complement(4851..>5767)
                     /locus_tag="PK710_17660"
     CDS             complement(4851..>5767)
                     /locus_tag="PK710_17660"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT11609.1"
                     /translation="CAILTALAEGHRLGILHRDIKPSNILFDQAGVTRLADFGAAHLS
                     DASHTATAGVIGTFAYMSPEQRLGMPATPASDVFGVGASLLEMLTGRPPAQPGSALTT
                     PSSCHPDLGPNHDAVVFALVADDPAHRIGHALDARTKLQALSWPDYNLGSLSPIEASP
                     VRHESRLQRLAPNLGFDVWLGRRVLLVADSPKMRQVMQAWTRTENRNLSAVLRYDVET
                     AMFWVEVPEGKTLSETARSLTKRQGEQLAAALGSLHENGVTHGAVDEHHLVMRDDRPV
                     LCLDPDTAMRGSPDMDRESLRRLCSHTT"
BASE COUNT         1153 a         1550 c         1829 g         1235 t
ORIGIN      
        1 acgtcgtctt gcggtaccgt gtcgccatga gacttcgatc agcaggctgg agcatcgctg
       61 tgacgatatc cctcctcggt gtccaaagcc cggcgaaggc aggagatttc gacgctcggg
      121 gcgtcttttc cttcgcttcc gatgcggtcg tgaaagaaag tttcgaagcg ttttctccga
      181 tcgaagcggg cggttcgaaa gccgagcccg tcgattcgca gaccgctctc gacggccaaa
      241 aaatcttgcg tctcaccctt gccaacgaag gactgcggct cgctgtcgat cttgtttccg
      301 gctttggcgt ggttcgattg tcgtattgga tgcgaggaga tgcggtaggg gggctcgcgg
      361 tagatagcga tgagcgtccg tcgaatctgg ctcaggcgta tccaaccggg cgggtcacga
      421 gcgatggttg gatggaaatg cgaacggccc cgatcgccat cgatgggcaa gccaaagggc
      481 tcgatgcgcg cgtgtttttt gcgggctacg acggttcgga aacgcggacc ttggacgtgg
      541 acgccgtgga aatcgaaagg ctcggggatg cggtggagcc gccgtcatgc caggggattg
      601 accttcaagg cgcctgtggt ccggaacaac tatgccttgc aggccgctgt cacgatgcgg
      661 cggtgtggtt tccccccctg cccgttgaag agaatcggga tcggctcatc gattattgga
      721 aacaaaaaat ccacgatacg tacggtccga tattgccaag aaaaaccgcg atgcccgacg
      781 ctttgcacac cctcgaggcc gctcgcaacg cttcctccaa tgtcgtgttt tggagccggt
      841 tcgcggaaag tattcgtcgt ttgcgcgatg cccacaccta tacgagaagt cctagcctat
      901 cccaactctc gttcggtcct ccgctaaacg tttgtttttt cgaaggaatc ggagaccgtt
      961 cgcaggcggc ctggccctcg gatcccagct ttggtgatat cctcgtgtct catacgggaa
     1021 cgagccacac ctggggcctg cgccagggcg accgattggt tgcggtcgac gggattcacc
     1081 ccgtcgcatg ggttcgatcg ctgatggccc atagtttatg gacatgggaa gcggatgaac
     1141 cgcgccaggt tgccaacacc gtatcgttgc ttcgacaact cataccgctg cacgctcgca
     1201 cggtgaccgt ggtgcattgt gatgccatgg cgaagtcctg cgatgacaag gccaccgtct
     1261 tgaccgtggc gcaaatggag ccggcctctc cggctctgga accgataagc tgtgacaatc
     1321 gcccatttta tcacgtgcct tcggccccgg agaaccacga ctttggcgat tcgttggctg
     1381 atgagtcggt cgtactcgaa gattggttgg atacctcgtc gcccgaagag ggcttacggg
     1441 ggatcgtgtg gaacagcctg ttgggaggat ggcctgggag caagctcgat gcgcaattgc
     1501 ggcaagcgac gaccacgtgg aaggccagtg cgcgtggtgt tttgatggat caccgtcagg
     1561 ggcatggcgg cacgatggta actgctgata ttctaataag tttttcgagg cagggcgctg
     1621 tgccgctcgc gagtttgttt cgtaatcgag cggcggatga agggcctcaa acgctcgagg
     1681 agggcaagga gttatttttg aaaattcgcg gctacgcggg gatccacgtg gggtccaacg
     1741 ctccgcaagt gcatattccg gtggcttttt tgacgacttg ggacgtatcg gcgagcgatt
     1801 ttctgcccta cctcatgaaa ggggcctccc aagtgcggtt gtttgggcca ggtccgacca
     1861 tgggagcgtt cggcacgttc tttcagtatt cctattgggg agggcttcga tggtccctcg
     1921 gcgcggaaga ttcgttgact ccggaaggaa tcacgttatg cggtcgaggg gtcgatccgg
     1981 atgaggtggt tttccccaag caatccgacc tcctcgaagg aaaagacacg gttcatgaag
     2041 ccgccgtgca ttggctccga aaggagatcg aaccatgaag cgcatcggta tgccttccgt
     2101 gctgttgtgg accgcgatga tcaccgtggg gtgcgactcg gcggcccctg acgtcaatcc
     2161 cgctcctcga gccgatgcgg ggacgcaaaa agaagcgtcg gtcgataccg gcctcgacgt
     2221 ggtggaggcg caagctccgg acgtggaaca agatgtcgtt gacgatgggg ggccgatgat
     2281 tcgacaggtt tatcagcgag atcctttcgg acgtctcgat ccgaccaacc tgttgcacga
     2341 tggggatttc gagttgtccg cggcggattc gatgcaatat ccatggcttg ggctaacggc
     2401 atcgaattac gtcacggggg ttgattgccg gtcggggttg cggtgtatcg agctttcccc
     2461 aagcgagtat gtggctgggg tgttcgtatg gccggagaca gcggcggtgg aggtatcatt
     2521 tcacggcaag ctccggtctt cgcaagattg tgagaaagag gcggtgggcg ttgtcatgat
     2581 ggccgatgcg tacgggggag aattgatgat tcccgcgaaa acctcggagc cggtggacgg
     2641 ttggtgtcag tatgccgccg aattatccgt gccctcgaat gttgggtatc atttgtgggt
     2701 gctgttgctc gccgcgcgcc aacaagccac cggagcggtg gttttcgatg atgtcacgat
     2761 ggtggcctcc tcgttccgaa ccggaaggca agcgctgtct gtaccgagcc aagacgatgc
     2821 atggctgaag caggctcgaa agaagataca gggctggctg ccgccgaatc cgccacggaa
     2881 accgctaccc attcgcaata tcacggccaa aaagaagctg tcgccaaacg cgggaatctg
     2941 atcttcgaaa cgcgaagaga aagaggcaaa gcaagaacta cggcatgcaa aacttcagcg
     3001 ccgccgtggc ttgcgtcgtg gtgcatgtgc cggccgcgca aggcgtgtag tcgtcgcaat
     3061 aggcccggca ataatacttg aaagggctcg agccgggctc caacacgcaa tcgaatccag
     3121 gctgacaaag gctcatattg ctgcagtcgg ctcctggcaa caatcctccg gccaaccaac
     3181 aatccgtgga gcccgtttcc gggtccatgt agtagcaccc ctcgttgtcg tcgcatcctt
     3241 tctggcgtac aggatcgcat ccgcttcggc acaagccgaa agcgacatcg tcaccccagg
     3301 gccaaacgca gtgcagatca tcgtcgttgc aatcgttgcc tccgtggcag aaagagcggc
     3361 aaacatactt gccccccgca tgcacgcaca tggctcccgg ttcgcattca tcgacggtac
     3421 agtctcctcc gactttgacg gctcccgcgg gcacacaggc ggtaaccgct cgtgaagcga
     3481 cactgagcac cgtacatcgt tcgtcggctt tgcaccctgt cgagggggga gagcagaggt
     3541 cgccgagaca gactcccgcg acggcgcctg ctgggccaaa ccatggttcg cacgattgtg
     3601 cggtaggttg cggacacgaa gccgtatcga tgcataacgg cctgcaggtc gatagcttgg
     3661 aactgtattc gacacaggcc aaaccgctga ggcaatcgtc gaatccgttt tccccgcaag
     3721 cgttgccgag ggatttgtca ccgtccggtc ggcataccaa cgcgtcgtct ttcccttcgg
     3781 ggctacactt ttccccaggt tcgcattctt ggcccaatag ggtgcaaggg cggtcgactt
     3841 gggcatccgg gtccgcgtcg atatcggtct cttgtggtgc gtccgaggct tcgacgacat
     3901 cctgggtggc gacatccgca ggggcttccg cttgctgatc cgcgatggaa tcggaggggg
     3961 catcgagcgt cgtatccgcc tcgacatccg tggtggcgtc ggttgtggca ccgcccgttc
     4021 ctccggtcgc gggtgcttga ttcgacgaat cctcatcgct gctgcaagcg aggatcgata
     4081 ggggaatagc taaaatagat aataaaaatc gaaacttacc catgtcgcac cccttgtgtc
     4141 cgcgcgtttg cgccttgccc agacaccttt gatccaaggg atgtttttca taggatgggt
     4201 cgagggtcaa gggaactgaa gagttcggag caggcgagac aaagggttgg ccgaggaggg
     4261 gcgaaccacc ggaagggcag ggcatatatc aagggcgtgg gagccattgt tcaggctatc
     4321 ttcatgccca agcgcggttg ggattggaat tttagcaagc gagcaggtca cgcaaggcat
     4381 cggccaatcg agaggcgatt cggtcttgca ggagacgata ggaccaagga atgtcgactt
     4441 cggcgagggc agcgccttcg cgcagcgaaa gtcgcgcttt tgctcctttg gccacgccgg
     4501 ggggaggtaa cacctccagg acgttatccg cccatcgcca cacgagccga tacttttccg
     4561 ccgcccgtat tgctgctcgt tccacgcggt ttcgtgcttc tcgtagtggc aaacgatggg
     4621 gatgaagcac gtgaaccgaa gtcattgtcg tcctacttgc tctgagcttg ctacgacacc
     4681 gccttcgttg ccatggagct tctgtggagg attggtgcaa aatcaatcat gcatgggcgc
     4741 cttgcatggt ggggatggac gctcgacggg ctcccagctt attcgaaggg aatggacgag
     4801 tacaaaaaag gatagaggga ttgggtcgtt ccagttatat aaaaatgtgg ttatgttgtg
     4861 tgactacaaa ggcggcgcaa gctttctcta tccatatccg gtgaacctct catggccgta
     4921 tccggatcga ggcaaagcac gggacgatcg tcgcgcatga ccagatggtg ttcatcgacg
     4981 gctccatggg tcacgccgtt ttcgtgcagc gagcccaacg ctgctgctag ctgttcgcct
     5041 tgtcgtttgg tcagggagcg cgcggtttcg gagagtgttt ttccttccgg cacctcgacc
     5101 caaaacatcg cggtttcgac atcgtagcgc aacacggcac tcaggtttcg gttttccgtt
     5161 cgtgtccatg cttgcatgac ctggcgcatc tttggcgagt cggcaacgag cagcacgcgt
     5221 cgtccgagcc agacgtcgaa gccaaggttt ggagcaagac gttgtaaccg gctttcgtgt
     5281 cgtacagggc tagcctcgat gggggacaac gagcccaggt tgtaatccgg ccagctcaac
     5341 gcttgcagtt tcgttcttgc gtcgagggcg tggccgatac ggtgggcggg atcgtcagcg
     5401 acaagagcga atacgacggc gtcgtggttc gggccaaggt cgggatggca tgacgatggt
     5461 gtggtcagag ccgatcctgg ttgggcaggg ggacgtccgg tcagcatttc gagcaagctg
     5521 gcgcccacgc cgaagacatc gcttgcggga gtggcgggca tgccgagtcg ttgctcgggg
     5581 gacatatagg cgaacgtgcc aatgacaccc gcggtagcgg tatgagaagc gtctgacagg
     5641 tgagcggcgc cgaaatccgc gagtcgggtc acgcccgctt gatcgaaaag gatgttcgaa
     5701 ggtttgatgt cgcggtgcag gatgccgaga cgatgtcctt ctgcaagggc cgtgaggatg
     5761 gcacaag
//
LOCUS       DAOSOR010000381         5761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14097_length_5761_cov_4.778304, whole genome shotgun sequence.
ACCESSION   DAOSOR010000381 DAOSOR010000000
VERSION     DAOSOR010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14097_length_5761_cov_4.778304"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PK710_17665"
     CDS             complement(<1..270)
                     /locus_tag="PK710_17665"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOT11610.1"
                     /translation="MEASGARDLNVRYRGRFAPSPTGDLHLGGASTALCAWLAARSVG
                     GSIVLRIEDIDTPRVRPDSEKHILEDLRWLGLDWDEEPEGILHQSQ"
     gene            complement(243..1748)
                     /locus_tag="PK710_17670"
     CDS             complement(243..1748)
                     /locus_tag="PK710_17670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11611.1"
                     /translation="MRPYRLRRMLAELPYWLVALGASAPAWIVKYPPIQDLPYHVATI
                     RVLHSFRDPYFGFSQHFELHLGNTHYLLYYLLGSALAYVIGVAFANVVLMSTYFAGTV
                     LALRSLLLAMGRDARISFFVVPLLTNVLFIYGLMPFLLGLPLMFWGLSVALRHTRKPC
                     WTTGLLLAGISVACFFTHIIPFGLLGIGIAAVFPWFSPTRWLRTLAPALPALALVTWW
                     TTATDAGRTTKGLLTQEAGVRKLDAALSEIPAWFTNVFRDDSDERWLIIYGLLVIATL
                     AVGLGQKEKLLPGSRRYALVPIACVVFYFTTPVGHDFIWPLAQRFAILVAITAIPLLA
                     FPRGWRGHVLTFAALVVAAGSTFDTARHFVQFQLEEVGDFDDAKAVIANNSKVCTLVF
                     DKGSQITHHQPFIHFGSWVQAEKGGVVMFTFAGYPHWPFDFLPGQAPPPGGPARRRWE
                     WTPERVRMEEIWPYYDYVLVRGSGFTPASTRFEKAYDGARWTVWKRVGQGT"
     gene            complement(2029..2244)
                     /gene="iscX"
                     /locus_tag="PK710_17675"
     CDS             complement(2029..2244)
                     /gene="iscX"
                     /locus_tag="PK710_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HOT11612.1"
                     /translation="MGGRAYMKWSDAREIAQALFDAHPDVKPLEIRFTDLHRWVCELD
                     DFEDDPDKSTESHLEAIQMAWLDEWED"
     gene            complement(2223..2549)
                     /locus_tag="PK710_17680"
     CDS             complement(2223..2549)
                     /locus_tag="PK710_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOT11613.1"
                     /translation="MAIVRYPKENLEVEVPVGFTILQASKKCNAPEGYACGGVCACST
                     CHVYVLSGAELLSEMEDEEDDILDKAFDVRENSRLGCQAKILEEGIIEVEISEESRKA
                     WEDEHT"
     gene            complement(2555..4480)
                     /gene="hscA"
                     /locus_tag="PK710_17685"
     CDS             complement(2555..4480)
                     /gene="hscA"
                     /locus_tag="PK710_17685"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HOT11614.1"
                     /translation="MSLIDIFDPKAAPKPIGIDLGTTNSIVAYVVDGQPRCVADCDGD
                     PRLPSVVHYGEHGQIIVGKEAIMLAPTHPRDTIASVKRFVGRGADDAETKLLGTYRFV
                     ASEDETDAKVIKMRVRDRIVNPVEVSAEILKQLKKNAEGVLRNVGGAVITVPAYFDDA
                     QRQATKDAGRLAGLEVLRLLNEPTAAALAYGLDGNKNGTFAVYDLGGGTFDITILRLD
                     DGVFQVRSTGGNSALGGDDMDRAMASVLLSEMGFDDPSERTPEIVRLAMDAARQTKHA
                     LTDQDVVDVELPAKGGATVCKRFTREAFDALIMPILEQTGPACRRALRDAELTPEALD
                     GVILVGGSTRIPAVRSFVQQMFGKEPLGNIDPDQVVALGAAVQADLLAGEGNADDVLL
                     LDVLPLSLGIETMGGVAEKILPRNTTIPAGAKQTFTTYADNQTGFEIHVVQGERELAS
                     DCRSLARFTLRGIPPMPAGLARLEVTFHVDADGLLHVSAREIKTGIEQQVEVKPSYGL
                     DEDQIEDMLLDALDHGEEDLAARRLAEQRVEAQRILMATRQGLDADGDLLNVVEHAHV
                     LTAIDALEASLKADKVAPIESAVAHLDMLTKAWAGRRMDRAIAKAIGGKGVEDVARSV
                     ENAGGIEAHLGQQTEKG"
     gene            complement(4477..4983)
                     /gene="hscB"
                     /locus_tag="PK710_17690"
     CDS             complement(4477..4983)
                     /gene="hscB"
                     /locus_tag="PK710_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HOT11615.1"
                     /translation="MNPFKILELEPRFDLDLDAAEHRHRELSRALHPDRFVGRPASER
                     RLALSRAIEVNEAWRTLRDPVRRAEALLNLHGIDSGETSSAKADPELLMEMMEQREAL
                     AETRASKDLPRLRDLSATMRDRQARVLDALTKAFSHDPQTALPVLAELRYVRRFLEEA
                     SALEDELL"
     gene            complement(5010..5342)
                     /locus_tag="PK710_17695"
     CDS             complement(5010..5342)
                     /locus_tag="PK710_17695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HOT11616.1"
                     /translation="MPITLTPAALEAARAALAKRGTPGAAIRLGIRGGGCSGFNYVIE
                     YEDDEPRSRDRVFETEGVRIVVDKKSLIYLMGTVLDFEKTLMKQGFKFRNPNEASSCS
                     CGTSFTVK"
     gene            complement(5573..5761)
                     /locus_tag="PK710_17700"
                     /pseudo
     CDS             complement(5573..5761)
                     /locus_tag="PK710_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
BASE COUNT         1197 a         1677 c         1619 g         1268 t
ORIGIN      
        1 ctgactttgg tgaagtatcc cttctggttc ctcatcccaa tcgagcccca gccaacgcaa
       61 atcctcgaga atgtgttttt cgctgtcggg ccgtacacgc ggtgtgtcga tatcctcgat
      121 tcgcaggacg attgagcctc ccacggatct tgccgccaac cacgcgcata gcgcggtcga
      181 ggctcctccc aggtgaaggt cgccggtagg ggagggcgcg aatcgtccgc gataccgcac
      241 gttcaagtcc cttgccccac tcgcttccat accgtccatc gtgcgccgtc ataggctttt
      301 tcgaaacgag tagaggcggg ggtaaatccg ctcccccgca ccaagacata atcgtaataa
      361 ggccatattt cttccattcg aacgcgctcg ggcgtccact cccatcgtct tcgggccggt
      421 cctccaggtg ggggagcttg acccggtaga aaatcgaaag gccaatgggg atagcccgcg
      481 aaggtgaaca tcaccacgcc tcccttttcc gcctgcaccc aggagccgaa gtgaatgaac
      541 ggttgatgat gggtgatttg cgatcccttg tcgaaaacga gagtgcatac cttgctgttg
      601 tttgcgatga ccgccttggc atcatcgaaa tcgccaactt cctcgagttg aaactgcacg
      661 aagtggcgag ccgtgtcgaa cgtcgaaccc gcggcgacca cgagggcagc gaaggtgagc
      721 acgtggccgc gccatccccg tggaaaggca agaagcggga tcgcggtgat ggccaccaga
      781 atggcaaacc gttgcgcgag cggccagatg aagtcgtgcc ccaccggcgt cgtgaagtaa
      841 aaaaccacgc aagcgatcgg caccaaggcg taacgccgtg atcccggcaa caatttttcc
      901 ttttgcccaa gcccgacggc aagggttgcg atcaccaaaa gaccataaat aatgagccag
      961 cgctcatcgc tatcatcgcg aaatacgttg gtgaaccatg cgggtatctc cgatagcgcg
     1021 gcatcgagtt tgcggactcc cgcttcctgc gtgagcaagc ccttcgtggt tcgacccgca
     1081 tccgtggctg tggtccacca cgtgacaagc gccaaggcgg gtagtgcggg agccagagtc
     1141 cgaagccatc gcgtgggtga aaaccaagga aaaacagccg cgattccgat ccccagcagc
     1201 ccgaagggga tgatatgggt gaagaaacac gctaccgaga taccggccaa tagcaatcct
     1261 gtcgtccaac aaggtttgcg cgtatggcgc agcgccaccg acaagcccca aaacatcagc
     1321 ggcaatccaa gaagaaacgg catcaggccg tagatgaaca agacgttggt caacaacggg
     1381 acgacaaaaa agctgattct tgcatcccgc cccatcgcca gcagcagcga acgaagggca
     1441 agcacagtgc ccgcgaagta ggtgctcatc aacaccacgt tcgcgaacgc gactccgatc
     1501 acgtaggcaa gcgcgctgcc cagcaaataa tatagaagat agtgggtgtt accgaggtga
     1561 agctcgaagt gttgactaaa accaaaatac ggatcccgga agctgtgcag cacgcgaatg
     1621 gtagctacat ggtacggaag atcttggatc gggggatact tgacgatcca ggcgggagcg
     1681 gatgcgccaa gggccacgag ccaatacggc aactcggcaa gcatacgtcg caagcggtaa
     1741 gggcgcattg tgggagacac tacaacggac aagggttgtc acgccacgac gttgactgcc
     1801 ggcgtctttt tggaaaactt acggtcagag gatgctgggt ggaatccgcg ggcatggcca
     1861 ccgtggtgat gtatcgggtc ctgggtggtc gatcgagcgg gtgaccaagt tctcaccagg
     1921 gatgtggttt atgtttccca acgtctttcg gaatcgatcc ccgatccatg aaagaatcca
     1981 cactatccga atacggatag aacgaagagg tcgatcacgc gccgtcgttt agtcttccca
     2041 ttcgtcgagc caggccattt gaatggcttc caggtgactt tccgtggatt tatcgggatc
     2101 atcctcgaag tcgtcgagtt cacacaccca tcgatgcaag tccgtgaacc gaatttcgag
     2161 cggtttcaca tccgggtgcg cgtcgaacaa ggcttgtgcg atctctcgtg catccgacca
     2221 cttcatgtat gctcgtcctc ccatgccttg cggctctctt ccgaaatctc cacctcgatg
     2281 attccctctt cgaggatttt agcttggcat ccgagtcgcg aattttctcg tacatcgaag
     2341 gccttgtcga ggatgtcgtc ctcttcatcc tccatttcac tgagcagttc tgctccggag
     2401 agaacgtaga cgtggcaggt ggagcacgcg cacacgcctc cgcacgcgta tccttccgga
     2461 gcattgcatt ttttcgaagc ctgcaagatc gtgaatccca cggggacctc gacttccaga
     2521 ttctcctttg ggtagcgaac gatcgccatt gcatctatcc cttctcggtc tgttggccca
     2581 agtgggcctc gatgccaccc gcgttttcga ccgaacgcgc cacgtcctcc acgccttttc
     2641 ctccaatcgc cttggcgatg gcccgatcca tccgtcgtcc cgcccacgct ttcgtcagca
     2701 tatccaggtg agccacggca ctttcgatgg gcgccacttt atctgctttc aacgatgcct
     2761 cgagcgcatc gatggcagtc aacacgtgcg catgctccac cacattgagc aagtctccgt
     2821 cggcgtcgag accctgccgc gtggccatga ggatgcgctg agcttccacc ctttgctccg
     2881 cgagtctgcg cgctgccaga tcttcttctc cgtggtcgag agcatcgagc aacatatcct
     2941 cgatctgatc ctcatccaaa ccataactcg gtttgacttc cacctgctgt tcgataccgg
     3001 ttttgatctc ccgcgcagac acatgaagca gtccatcagc atcgacatgg aacgtcactt
     3061 cgagccgtgc taggccagcc ggcatgggcg ggatgcctcg cagcgtgaag cgcgccaacg
     3121 accggcagtc actcgcaagc tcccgctctc cttgcaccac atggatctcg aatcccgtct
     3181 gattgtccgc ataggtggtg aaggtctgct ttgctcccgc ggggatcgtg gtgttcctcg
     3241 gtagaatttt ctcggccacg ccacccatcg tctcgatacc aagcgatagc ggaagaacgt
     3301 cgagcaacag gacgtcatcg gcgttgcctt cccccgcaag caagtccgcc tgcaccgcgg
     3361 ccccaagagc caccacttga tccggatcga tatttcccaa cggctctttg ccaaacatct
     3421 gctggacaaa cgatcgcacc gctggaattc gtgtcgaacc tccgaccaaa atgaccccat
     3481 ctagcgcctc tggtgtgagt tccgcatcgc gcaatgcccg acggcaggcc ggtcccgttt
     3541 gctctaagat cggcatgatc aaggcgtcaa acgcctcacg cgtgaatcgt ttgcatacgg
     3601 tggctccgcc tttggcgggc aattcgacat ccacgacatc ctgatcggtc agtgcatgtt
     3661 tggtttgtct tgctgcatcc atggcgagac gaacgatctc gggtgtacgc tcgctgggat
     3721 cgtcgaatcc catttccgaa agcaataccg atgccatggc gcgatccatg tcatcgccgc
     3781 ccagcgcgct atttccgcca gtcgagcgga cctggaacac gccatcatcg agtcgcaaaa
     3841 tggtgatgtc gaacgtaccc ccacccagat catagacagc gaacgtaccg tttttgttgc
     3901 cgtcgaggcc gtaagcgagc gcggcggcgg taggctcgtt gagcagacgc agcacttcga
     3961 ggccagcgag tcggccggcg tctttcgtgg cctgtcgctg cgcatcatcg aagtaagccg
     4021 gtacggtgat gacggcgccg cctacgttac gcagcacgcc ctccgcgttt tttttgagct
     4081 gtttcaggat ttccgcggat acttccaccg gattcacgat ccggtcccgc acacgcatct
     4141 tgatgacctt ggcgtcggtt tcgtcttcag atgccacgaa tcggtaggta ccgagcaact
     4201 tggtttcggc atcatcggcg ccgcgcccca cgaatcgctt cacactggcg atcgtgtctc
     4261 gagggtgggt gggcgcgagc attatggctt ctttgcctac gatgatttgt ccatgctcgc
     4321 cgtaatggac gacagagggc agccgagggt ctccatcaca atcggcgaca caacgaggtt
     4381 gaccatcgac gacataggcg acgatggaat tggtggtgcc aaggtcgatg ccaatgggtt
     4441 tgggagccgc ttttgggtcg aagatatcga tgagactcac agcaactcat cctccaaggc
     4501 gctggcttcc tcgaggaagc ggcgcacata acgaagttct gcaaggaccg gaagggcggt
     4561 ttgcgggtcg tgggaaaatg ccttggttag cgcatctagt acgcgcgctt gtctgtctcg
     4621 catggttgcg gatagatctc gcaatcgcgg aaggtctttg gaagcacggg tttccgcaag
     4681 ggcttcccgc tgctccatca tttccatgag tagttcgggg tccgccttgg ccgacgatgt
     4741 ttctccgcta tcgatgccat gaaggttcaa caatgcttcg gcgcgtcgga cgggatcgcg
     4801 aagcgtgcgc caggcttcgt tgacctcgat ggcgcgcgac agggcgagtc ttcgttcgga
     4861 agcgggacgt cccacgaagc gatctggatg aagggcgcga gacaattccc gatgtcgatg
     4921 ttcggcggca tcgagatcga ggtcaaaacg aggttcgagt tcgaggattt tgaatggatt
     4981 catcaaaggc aactcccggt gcggggagtc tacttcacgg tgaaagaggt accgcaacta
     5041 caagaggagg cttcattggg atttcgaaat ttgaatcctt gcttcatcaa cgttttttcg
     5101 aagtccaata cggtgcccat cagataaatc aggctttttt tatcgacgac gatacgtact
     5161 ccctccgttt cgaatactcg atcccggctg cggggttcgt catcttcata ctcgatgaca
     5221 taattgaatc cgctgcaccc cccgcctcga atgccgagcc gaatggcggc cccaggcgtg
     5281 ccccgcttgg ccaacgccgc gcgggccgct tccagtgccg cgggcgtaag cgtgatcggc
     5341 actttttttc cttccgattg ttctgccgaa ctcgcttgtt ccgatgtttt ggcctcgaca
     5401 ttgtccgtca tgatgattgt cctggatgcg gggaatgacc gtggatggct caccgttcgg
     5461 gttgcgatga cacgatggga agtgagcgct ttccctccga tggcgcatta ggcgtttctt
     5521 gtgcttcctg cgcgggttcc ggatcgtctt gcgtttgggt ttggagggca cgtttggctt
     5581 tttgcttccg acgaaaatcc tcgatggcgc ttttgatggc atcttcggcg agtacggagc
     5641 aatgaatttt caccggtgga agattcagtt cttccgcgat atcgcggttt ttgattttgg
     5701 ttgcttcctc gagggtgcga cctttgagcc attcggtggc gagcgaagag gaggcaatgg
     5761 c
//
LOCUS       DAOSOR010000382         5757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14111_length_5757_cov_4.320239, whole genome shotgun sequence.
ACCESSION   DAOSOR010000382 DAOSOR010000000
VERSION     DAOSOR010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14111_length_5757_cov_4.320239"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PK710_17705"
     CDS             complement(<1..383)
                     /locus_tag="PK710_17705"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOT11617.1"
                     /translation="MWPRRLWFLGISTILTLLSWEGLSFAQTETTVHTVQQGQTLGAI
                     ARRYRTSVDALCKANRIRKTDPIRPGQKLVVPNPQPPAQSTPTPSVRPDSEWQRDLQT
                     LEVSGAPSAYYYEPAGPKRSGPRPV"
     gene            complement(462..3152)
                     /gene="alaS"
                     /locus_tag="PK710_17710"
     CDS             complement(462..3152)
                     /gene="alaS"
                     /locus_tag="PK710_17710"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOT11618.1"
                     /translation="MSHLTASAIRSAFLDYFRRHDHEIVASASLIPSNDPTLMFNNAG
                     MVQFKDVFTGRETRPYVRAASSQKCIRISGKHNDLENVGKTARHHTFFEMLGNFSFGD
                     YFKEKAILFAWEFLTKDLGLPPERLAITVFGGENGIPADDDARVIWRKVTGFGDDRIL
                     GMGMKDNFWQMGDTGPCGPCSELHYFVGNDVDLSKFGQDPTDDGKGWMEIWNLVFMQF
                     NRTLDGAEAKLSALPAPCVDTGAGLERLAAVLQQVSSNYETDLLMPLVEKAASISNKK
                     YTHSQADDDTSMRVIADHARTAAFLIAEGILPDRDGRSYVLRRVVRRAIRHGNRLGIH
                     RPFFFEVALDVVERMGACYPELVERRDLIADALEQEEVRFRKTLDNGLRILEDEFSGL
                     EHKGQKVLSGDVAFKLYDTYGFPLDLTQVICEERRITVDQAGYKLALEQAQARSKGSE
                     VGDKALPRAYFDALEAVTNKSVRFLGYETERSDSARVVAIIVDNALVEEAPAGTTAEI
                     VLDATPFYGESGGQVGDTGVFLRNGELAARVFDTQRPLDALWVHKVEVVAPVRIGDGL
                     EAIVDHEARQATRRNHSATHLLHHALRSVLGPHAAQKGSLVSPDRLRFDFTHGKAVTP
                     QELQQIEDIVNDKILRNVPVETHILPLEEARTLGAIAIFGDKYGDTVRVLTITPESVE
                     LCGGTHARASGDIGLFKIISEAGIAAGVRRIEAVTGLNALGYVRQLEQTVQQASKLVK
                     ADGPDLADKVLKLTQHEKELEKQIADLKRKVAMGGGGVDSMLQQAHEIGGMKVLAVRS
                     EVADGAALREMAEKLRDTLGDAVVVVGAAAGPKAMLVATVSKNLTHKIKAGDIIRQVA
                     QQVGGSGGGRPDMAQAGGSDPSKLDEALKSVTAIVQSLLS"
     gene            3276..3680
                     /locus_tag="PK710_17715"
     CDS             3276..3680
                     /locus_tag="PK710_17715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11619.1"
                     /translation="MIARRFFALCGWVVALGGCGDDKQSVDASQVFDSGQGTNSQSFC
                     QDYCTTLVEVAPGCEQYNENHRCEKICKYYVASVCSEPYEAFASCLQQSKKGQCFQPD
                     AGRLTLVVDACHEPYQRWQTCIQEKNASVCPY"
     gene            3821..5431
                     /locus_tag="PK710_17720"
     CDS             3821..5431
                     /locus_tag="PK710_17720"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT11620.1"
                     /translation="MSTKAQSIQTRQSLRPQMVVGDKYRLDSLLAEGGMGTVWVATNL
                     VLERKVAIKVLHGEARNDLAEARLLREARAAAQIGHTNIVQVFDFGHTDLGEPYIVME
                     LLRGEDLSVRLARVGRLAAADAVKLLLPVTSALAAGHAKGIVHRDMKPGNVFIAIDDV
                     GNEIPKVVDFGIAKVQTPQYTPKLTVEGRVVGSPEYLSPEQARGEDDLDATTDVWALC
                     VTLYETLTGNIPFRDENYNRLLRRIIEDPPRPITDYAAGDAALWSIISRGLCKHRRAR
                     WASMEELGRAMEEWLEQRGVEDIPRVRFASLSRVAQDFPAHPPASSNRSNTTDDGEDA
                     EPTILKLPPPVPGPAPSLPSQELSVPLPGLTVPSSVNASPLPVHRDPSPSRVEAPPVY
                     GDLHGSGSSLSKLPSRQLQLAVMIVGGFIIGVTAVVLIAIAVTRLSSDEHPSREPMAS
                     VSDSSPMPMQTSSIQIPPVVAEPAETQGPEPSVTMTAGLDAAPAPSPPMPSTTALSSS
                     TTTPSGRPPRPIRNWTTSSPPIPKDPNF"
     gene            5440..>5757
                     /locus_tag="PK710_17725"
     CDS             5440..>5757
                     /locus_tag="PK710_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11621.1"
                     /translation="MRRAFIPVLMLAGAVAPLGCSLFVASELEDKSVTRADAGSSDAA
                     TDRDADASMSLDAFSDKAEASEDGTSDSQEDSQEEDEGPPDAGDCSQCQFKCCDNRCV
                     DTSS"
BASE COUNT         1171 a         1571 c         1713 g         1302 t
ORIGIN      
        1 aatacgggac gcggtcccga gcgtttcgga ccggctggtt cgtaataata ggcagaaggt
       61 gcacccgaaa cctccagggt ttgcaaatcg cgctgccatt cgctgtcggg ccgtactgat
      121 ggtgtgggcg tggattgggc agggggttgg ggattcggaa ccaccagctt ctggccggga
      181 cgaatcggat ctgttttccg gattcggttg gctttgcata aagcatcgac ggaagtccgg
      241 taacgacgag cgatggcgcc caaggtttgg ccttgttgca ccgtatgcac ggtggtttcg
      301 gtttgggcga aagacagccc ctcccacgaa aggagcgtga gaatcgtgga tattccgagg
      361 aaccacaaac ggcggggcca catcacggag gttcgacgta gcacaaggga tgggattcag
      421 tgaatctttg gggattttat cggggaaagc ggcgaattgg gctaggaaag gaggctttgc
      481 acgatagcgg tcacggactt caaggcttca tcgagtttgg atgggtccga gccgcccgct
      541 tgcgccatgt ctggacgccc tccgccggag ccgcctactt gttgcgcgac ctgacggatg
      601 atgtcccctg ctttgatttt atgggtgagg tttttgctga cggtcgcgac gagcatggct
      661 ttggggcctg ccgcggcgcc aacgacgacc acggcatctc ccagcgtatc acggagcttt
      721 tccgccattt ctcttagtgc ggccccgtcg gccacctccg accgcaccgc aagcactttc
      781 attccgccga tttcatgggc ttgttgaagc atcgaatcca cgccaccacc acccatcgca
      841 accttgcgtt tcaagtccgc gatctgcttt tccaactctt tttcatgttg tgtgagcttc
      901 agaaccttgt cagcaaggtc cgggccgtcg gcttttacga gttttgaagc ctgttgcacc
      961 gtctgctcga gctgccgaac atagccgagg gcattgaggc ccgtgacggc ttcgattctt
     1021 cgcaccccag cggcaatccc tgcctcgctg atgattttga aaaggccgat gtcccccgaa
     1081 gcgcgagcgt gggttcctcc acatagttca accgactcgg gcgtgatggt gagcacgcgc
     1141 acggtatcac catacttatc gccaaagatg gcgatggcgc ccaaggtccg cgcctcctcc
     1201 aacggcaaga tatgcgtttc gaccggcacg ttgcgcagga tcttgtcatt gacgatgtct
     1261 tcgatttgct gcagttcttg cggggttacc gctttaccat gggtgaagtc gaagcggagt
     1321 cgatcggggc tgaccaacga acctttctgt gcggcatgcg gaccgagtac ggagcgaaga
     1381 gcatgatgga gaaggtgcgt tgcggagtga ttccgacgcg tggcttgtcg tgcttcgtgg
     1441 tcgacgatgg cttcgagtcc gtcaccgatt cgtacgggag ccacaacctc gaccttatga
     1501 acccacagcg catcgagggg gcgctgggtg tcgaataccc gcgcggccaa ttcgccattt
     1561 cgaagaaaaa cacccgtatc gccgacctgg cctccggatt cgccatagaa aggcgttgca
     1621 tcgaggacga tttctgccgt ggttcccgcg ggtgcttctt cgaccaaggc attgtcgacg
     1681 atgatcgcta ccacgcgagc tgaatcggag cgctcggttt cgtagcccaa gaaacgaacg
     1741 gatttgttcg tcacggcctc gagcgcgtcg aagtaggcgc gcggaagcgc cttgtctccg
     1801 acttcactgc ctttgcttcg tgcttgggcc tgttcgaggg ctagcttgta gccggcctgg
     1861 tccacggtga tcctacgctc ttcgcagatg acctgggtaa gatcgagggg aaaaccgtac
     1921 gtatcataga gtttgaaagc aacgtctccc gacagaacct tctgcccttt gtgctcgagg
     1981 ccagagaatt cgtcttcgag aatccgcaat ccgttgtcca acgtctttcg aaacctcact
     2041 tcttcctgct cgagagcatc agcaatgaga tcgcgtcgct ccacgagttc cgggtagcaa
     2101 gcgcccatac gctccaccac gtcgagggcc acctcgaaga aaaagggacg atggataccg
     2161 aggcggtttc cgtgtcgaat cgctcggcgc acgacccttc gcaatacgta ggaacggcca
     2221 tcgcgatcgg gcaggatccc ctcggcgatg agaaatgcag cggtgcgagc gtgatcggca
     2281 atgactcgca tggatgtgtc atcgtcggct tgggaatgcg tgtatttttt attcgaaatc
     2341 gatgcggctt tttcgacgag gggcatgagc agatcggttt cgtagttgga agagacctgt
     2401 tgcaggacag cggcaagtcg ttcgagaccc gctccggtat cgacgcatgg ggcgggcagg
     2461 gcagagagct tggcctcagc gccatccaac gttcggttga actgcatgaa caccaagttc
     2521 caaatctcca tccacccttt tccatcgtcg gtgggatcct gtccgaactt gctgaggtcg
     2581 acatcgttgc cgacgaagta gtgaagttcg gaacaggggc cgcaggggcc ggtgtcgccc
     2641 atctgccaga agttgtcttt catccccatg ccgaggatgc ggtcatcacc aaatcctgtt
     2701 acttttcgcc atattacacg cgcatcgtcg tcggcgggaa taccgttttc tcctccgaag
     2761 acggtgatag caaggcgttc gggcggcagt ccaaggtctt tggtgaggaa ctcccaagcg
     2821 aagaggatgg ctttttcctt gaagtaatcg ccgaagctga agttgccgag catctcgaaa
     2881 aaagtgtgat gacgtgcggt tttgccgacg ttttcgaggt cgttatgctt tccgctgata
     2941 cggatgcatt tttggctgga tgccgcacga acgtacgggc gcgtttcgcg ccccgtgaac
     3001 acatctttga actgaaccat cccggcgttg ttgaacatca gggtggggtc attggagggg
     3061 ataagagagg ccgaagccac gatctcatga tcgtgtcgtc gaaaataatc gaggaaagca
     3121 cttcggattg cagaggcggt cagatggctc atggtcgatg ttcgaggctg aagggttttg
     3181 acaatcggat gcgttcctac cccgaagacg attcgttcga aagggggtga gcgcaaggtt
     3241 ggggcgagtt cggttctccg tgctattcta ccgtgatgat tgctcgacgt tttttcgctc
     3301 tgtgtggatg ggtcgtcgcg ttaggggggt gtggcgacga taagcaatcg gtggatgcga
     3361 gccaggtgtt cgattccggg caaggaacga atagtcagtc attttgccag gactattgca
     3421 ctactttggt agaggtggct ccaggctgtg agcaatacaa cgaaaaccat cgatgtgaga
     3481 aaatctgtaa gtattacgtg gcttctgtct gtagcgagcc gtacgaggcg tttgcctcgt
     3541 gcctgcaaca atcgaaaaaa ggtcaatgtt ttcaaccaga tgcgggtagg ttgaccctgg
     3601 ttgtggacgc ctgccatgag ccgtatcagc gttggcagac ctgcattcaa gaaaaaaacg
     3661 ccagtgtttg cccttattga ggagcgcggg tgcgaagcgg gtccaaagcc ttgtgcggcc
     3721 ctgcaaatcg cccagaaaaa gacgtcggcg tgcgaacgtg ataccctgtc ttccagcgtt
     3781 ttgccgactg gcaagattgg tatacgattc aaggtcgacg ttgagcacga aggcccaaag
     3841 catccaaacc cgacagtcgt tacgacctca gatggtcgtg ggcgacaagt accgccttga
     3901 ttctctgttg gcggaagggg gtatgggcac ggtgtgggtc gcgaccaacc tggtgctcga
     3961 acgcaaggtt gccatcaagg ttttgcacgg cgaggcacgc aatgatctgg cagaagcccg
     4021 tttgttgcgt gaggcgcgcg cggcggccca aattgggcac acgaatatcg ttcaagtttt
     4081 cgatttcgga cacacggatc tgggcgagcc ctacatcgtg atggaattgt tgcggggcga
     4141 ggatctgagc gtgcgacttg cgcgtgtggg gcgccttgcc gctgccgatg ccgtcaaact
     4201 cctgcttccg gtgacgagtg cgctcgccgc cggccacgcc aaaggaatcg tccatcgcga
     4261 catgaaacca ggcaatgtat tcattgccat cgatgatgtg ggtaatgaaa tccccaaagt
     4321 cgtcgatttt ggaatcgcaa aagtacaaac ccctcagtac accccgaaac tcaccgtcga
     4381 aggacgtgtg gtcggcagtc ccgaatacct atcccccgaa caagctcggg gggaagacga
     4441 cctggatgca accacggatg tgtgggccct atgtgtcacg ctttacgaga cgcttacggg
     4501 caacatcccg tttcgcgacg aaaactacaa tcgtttgctt cggcgcatca tcgaagatcc
     4561 gccccgtccc attacggatt atgcagcggg agatgcggcc ctgtggagca ttatttcacg
     4621 agggttgtgc aaacatcgtc gcgctcgctg ggcatcgatg gaagagttgg gtcgcgcgat
     4681 ggaagaatgg ctcgaacagc gtggggtaga ggatattcca cgggttcgtt tcgccagtct
     4741 gtctcgcgtc gcacaggact ttccggctca tccccctgcc agttcgaatc gatcgaatac
     4801 gaccgacgat ggggaggatg cggagccgac gattttgaag ttgcctccgc ccgttcctgg
     4861 gcctgcacca tctctgcctt cccaggagtt gtcggttccc ctaccgggat tgacggtgcc
     4921 aagcagtgtc aatgcaagtc cgctgcccgt tcatcgcgat ccttcgcctt ctcgcgtgga
     4981 agctcctccc gtctacggcg acttgcatgg ttcgggctca tcgttgtcca aacttccctc
     5041 tcggcagttg caactcgcgg tcatgatcgt gggtggattc atcattggag tgaccgcggt
     5101 cgtgctgatt gccattgcgg tgacaaggtt atccagcgac gagcatcctt cgcgggagcc
     5161 tatggcatcg gtttcggact cttctccgat gccgatgcaa acctcttcca tccagatccc
     5221 accggtggtc gcggaaccag ccgaaacgca agggcccgag ccgtctgtaa ccatgaccgc
     5281 gggtctggac gctgctcctg cgccaagccc cccgatgcca tctaccacgg cgctttcttc
     5341 ttccaccacg acaccttcag gccgtccacc acggcctatc cgaaattgga caacgtcttc
     5401 gcctcctatc cccaaggacc ccaacttctg aggtttgcaa tgcgtcgagc gttcattccg
     5461 gttctcatgc ttgccggagc cgttgcgccc ttgggttgct cgctttttgt ggcaagtgaa
     5521 ctcgaggata aatcggtcac cagggcggat gcgggctcga gcgatgccgc gacggatcgc
     5581 gacgcggatg caagcatgtc cttggatgct ttctcagaca aagcagaagc ttccgaggat
     5641 ggaacgtcgg attcgcaaga agattcgcaa gaagaggatg aagggccacc tgatgccggc
     5701 gattgctccc aatgccagtt caaatgttgt gacaatcgtt gtgtcgatac cagttcg
//
LOCUS       DAOSOR010000383         5739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14168_length_5739_cov_3.843068, whole genome shotgun sequence.
ACCESSION   DAOSOR010000383 DAOSOR010000000
VERSION     DAOSOR010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14168_length_5739_cov_3.843068"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PK710_17730"
     CDS             <1..547
                     /locus_tag="PK710_17730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT11622.1"
                     /translation="EHGGAIALHTAPGQGCSFRVLLPCYESEEKGDNDKASAPLHGKE
                     RIVVVDDAQQVVRAIRRMLEPLGYTVEGFTDPKEALSFFRGPSNFADLLITDLVMPEM
                     TGIELSEAVLAINPKLPILLVTGQNTNQIQSVTEHSSIRSVLFKPLDPIVLARTIRHQ
                     IDLGTAGDGEEKPAHNVDRRS"
     gene            complement(548..1636)
                     /locus_tag="PK710_17735"
     CDS             complement(548..1636)
                     /locus_tag="PK710_17735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11623.1"
                     /translation="MNSFRTILVVAALTAIAAPICLGCEENSSKPSPSSKPKPDDETV
                     AVDPAIGKVLAASSASPDTAPSPTQDDGPPATGVFSPDRANATHAVNAPIHIEVGTLG
                     SEPRIRLLPTSMDVPKSLSVTIATQLGARTSLPTVDIVLGLTAPKPKSQGDEAPDLAL
                     PVSAKISKISLSAQQPGQIPKEIADEVAKMKDSKASWSFEPNGGIRIGSIERSPKARP
                     ELEHNLVVLMETLAAASIAAPKEPVGVGATWIARSRDVYGGLDLVSYRMFKVAKIDGE
                     NVSLVVETRQYAASDDLQKPGLPEGAKLVQFESLGAGEFIVTAGHRFAESGSYSHGMK
                     FGLRPPNAADNQLMPLILQSTVAFPIAR"
     gene            complement(1751..3028)
                     /locus_tag="PK710_17740"
     CDS             complement(1751..3028)
                     /locus_tag="PK710_17740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT11624.1"
                     /translation="MSNHDITESPLRANRSFQGHVATQFFGAFNDNLFKQLILFLAAR
                     ELFPGQDKQGIAFAVFSLPFVLFSGVAGDLSERFSKRTIIVLMKVAEIGIMLAGAVAL
                     QQRAWTAMLVVLFIMGAQSAFFGPSKYGIIPELVGKQRLLSANGIISMTTFLSILLGQ
                     AAAGPLLDRYGQQLWITGAWCVGVAVVGTGVALLIRPLPALRPTMPIRKNPFGSVIDG
                     FRQLRSDPRVIHVLLLNSLFWFNGGVVQQALVGIGAPAYLDIPLDQNWRLSLLLVSLA
                     TSIIVGSVCVPIVAKAIRPGKLIAFGAVAMLVCQASLTVSISAWGPQAYSTCVVLVAI
                     LGFTGAFFAVPVQTFLQDAPEQGTRGITFAVNNFLNFSFIFLAGAFYLTSAKIQLSPG
                     FAATLAAVAMCSVLWVVRKSVLGIERPNATSTP"
     gene            3185..4954
                     /locus_tag="PK710_17745"
     CDS             3185..4954
                     /locus_tag="PK710_17745"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT11625.1"
                     /translation="MSASQFQPGFRVGRYELLAPIASGGMASVWAARIQGTRGFEKLV
                     AIKTILPHLADDPQFETMFLDEARIASEIVHPNIAQVFDLGEQDGVLFLVCEWVDGES
                     LATLRKRVVDQGQEIPIPIALRIMSDVCGGLHAAHELRDVTGKPLHVVHRDVSPQNIV
                     ISDAGAVKVIDFGVAKAQNRKLDQTVAGKLKGKVQYMPPEQARGLPVDRRTDLWSVGA
                     VLYHLLAGRTPYEADSPLAVLNLLLSEEAPPPLPAHVPESVADIVFQALRPNPIERFQ
                     TAAEMQIAIAMALRNVSMPIPDAAIGEFVRRYVGAQAKQRHQLIAEAIEAANHAPPPP
                     ADLTLVEVVEAVEELISKEKEPSSPASPPEAVRPPPHEKVGQTLVAPFPAPAQPPIEP
                     ENEEPVLLVRRSRADWTPTSAPTVVEHPVVNEPYPMAQDNRAPSLAPSAAPSPAQSPP
                     RPTPNPSTTAVTDPAITGPRRAVALPAVPQILVAVVVFLAVIATFALFWPKTKPPLGS
                     SPALSASETAKAVLPQSSAEPNPSEHDDIPIVSGSDLPTVAPSIPDSSRLRPKRPLPG
                     STVPPPPPGWESLPSLTLEPESP"
     gene            5219..>5739
                     /locus_tag="PK710_17750"
     CDS             5219..>5739
                     /locus_tag="PK710_17750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11626.1"
                     /translation="MATLFETDLWTASAKREPSSFYRPLTMVTFWINAWIAGPSATSM
                     RAGNVMVHATNALLLWLLLRRLLSPTRTLLSASIAGLWAVMAVNSEPVLWISGRFDPL
                     TVTVALGCMLANRLRSVWGTVFVVLLATCGLFIKETFMGWLPLLFLDDVWLLGRHPKQ
                     SIGKAIGLAVVIG"
BASE COUNT         1202 a         1579 c         1723 g         1235 t
ORIGIN      
        1 ggaacacggt ggtgcgatag cccttcatac cgcccccgga cagggttgct cgttccgagt
       61 gcttttgcct tgttacgaaa gcgaagaaaa gggtgataac gacaaagcaa gcgccccgct
      121 ccacggcaag gagcgaatcg tggtagtcga tgacgcacaa caagtcgtgc gcgcgattcg
      181 aagaatgctc gaaccgcttg ggtacaccgt cgaaggattt accgatccga aagaagctct
      241 atcttttttc cgcggccctt cgaatttcgc cgacctcctg atcaccgatt tggtgatgcc
      301 cgaaatgacg gggatcgagt tgtcggaagc cgtgttggcg ataaacccga agctgcccat
      361 cctgctggtg acaggacaaa ataccaatca aattcaatcg gttacagaac attcatccat
      421 tcgttccgtg cttttcaaac ccttggatcc gatcgtattg gcgcggacca ttcggcacca
      481 aatcgacctt gggactgccg gtgatggtga ggaaaagcca gcgcacaacg tcgatcgtcg
      541 ttcgtgatta tcgggcgatg gggaatgcga cggtcgattg gagaatgagg ggcatgagtt
      601 ggttgtcggc ggcgttgggg gggcgtaaac cgaatttcat tccgtgggaa taggagccgg
      661 attcagcgaa tcgatggcct gcggtgacga tgaattcccc ggctccgagg gattcgaatt
      721 gcacgagttt ggctccttcg ggcaatcctg gtttttgcag gtcatcgctt gcggcgtatt
      781 gcctcgtttc cacgacgagg ctgacatttt caccatcgat ttttgcgact ttgaacattc
      841 ggtacgatac gagatcgagg ccgccgtaaa cgtcacggga gcgagcgatc caggtggccc
      901 ccacccccac gggttctttg ggggcggcga tcgaggcagc ggccagtgtt tccatcaata
      961 cgaccaggtt atgttccaat tcgggtcttg ctttggggga tcgttcgatt gacccgatcc
     1021 ggataccgcc gttgggttcg aaggaccagg aggccttgct atccttcatt ttggccacct
     1081 cgtcggcaat ctctttgggg atctggccgg gttgctgcgc agacagggag atttttgaga
     1141 ttttcgccga aaccggcagg gcgagatccg gtgcttcatc gccttgggat ttcggtttcg
     1201 gcgcggtcag tccgagcacg atatccacgg taggcaagct cgtgcgggct cccaattggg
     1261 tggcaatcgt cacagaaaga gatttgggaa catccatcga agtaggcagc aggcgaatac
     1321 gaggttcgga gccgagcgta cccacttcga tatgaatggg agcgttgacc gcatgggtgg
     1381 cgtttgcccg gtctggggaa aacacgcccg tggcaggcgg accatcgtct tgcgtgggcg
     1441 atggcgccgt atcgggtgaa gctgaagagg cagcgagcac ttttccgatg gccggatcga
     1501 ctgccacagt ttcatcgtct ggcttgggtt tggaggaagg cgagggtttg gaggagtttt
     1561 cttcacatcc aaggcatatt ggcgcggcga tagcggtcag ggcggccacc accagtatgg
     1621 ttctgaatga attcatgacg ataggattta gcgacatagt ggatggtcgg caagaggatc
     1681 gaaaggagcc gaaatatcct agatggtgga gcgctgcggg gttgtcggac gactctttgg
     1741 cctgcgatgc tcagggggtg gatgtcgcgt tgggtcgttc gattccaagg acgctttttc
     1801 ggaccaccca aaggacggag cacatcgcca cggccgcgag ggttgcggcg aatcctgggg
     1861 agagctgaat tttggcgctt gtgagataaa aggcgcccgc gagaaaaatg aaactgaagt
     1921 tgaggaagtt gttgacggca aacgttatgc ctcgggttcc ttgctctggg gcgtcttgca
     1981 aaaacgtttg cacgggaacg gcgaaaaacg cgccggtgaa tccgagaatc gcaaccagta
     2041 cgacgcacgt cgaataagcc tggggtcccc acgcggagat gctgacggta aggcttgctt
     2101 ggcaaacgag catggccacg gcgccaaagg cgatcaattt ccctgggcga atggcctttg
     2161 ccacgatcgg cacgcaaacg cttccgacga tgatggaggt tgccagagat acgagcagca
     2221 atgacaggcg ccaattttgg tcgaggggga tgtcgagata cgcgggtgcg ccgattccca
     2281 cgagggcttg ttgcaccacg cctccattga accagaacag cgaattgagc agcaatacat
     2341 ggatgacccg tgggtcggaa cgcagttggc gaaagccgtc gatgacgcta ccaaacggat
     2401 tttttcggat gggcatggtg ggacgaaggg cgggtagagg ccgaatgagc agggccacgc
     2461 cggttccgac gacggctaca ccgacgcacc aggcgccggt aatccaaagc tgttgaccgt
     2521 agcggtcgag caacggtcct gcggcggctt ggccgagcag gatggaaagg aaagtggtca
     2581 ttgaaatgat gccgttggcg gaaagaaggc gttgttttcc caccagttcc gggatgattc
     2641 cgtatttgga agggccaaag aacgcggatt gtgcgcccat gatgaacagc acgacgagca
     2701 tggctgtcca cgcgcgttgt tgcagtgcga cggcgccggc tagcatgatg ccgatttcgg
     2761 ccactttcat gagaacgatg atggtgcgtt tgctgaatcg ttcgctcagg tctcccgcca
     2821 cgccgctgaa aagaacgaaa ggcagagaga agaccgcgaa agcgatgcct tgtttatcct
     2881 gtccgggaaa caactcgcgg gcggccaaaa ataaaatgag ttgtttgaat aggttatcat
     2941 tgaaagcgcc gaagaattgc gttgcgacgt ggccttgaaa gcttcggttg gcgcggaggg
     3001 gcgattcggt gatgtcgtga ttcgacatgg cgagaaaggg aaacagagtt ggaactgaag
     3061 gtcaaaccca cgccatgggc ggagggttga cgacaggcag gggcctgagt ttgtgggaat
     3121 accacggctt gggtccatct gggttgctaa cgacgagagt ttcgaacagg atgccgctgg
     3181 tcggatgtct gcatcgcagt ttcagccagg ttttcgtgtc ggccgctatg agcttctcgc
     3241 gcccattgct tccggcggaa tggcatcggt gtgggcagcg cgcattcaag gcacgcgtgg
     3301 attcgagaag ctggtggcga tcaaaacgat cctgcctcat cttgccgacg atccgcagtt
     3361 cgagacgatg tttctggacg aggctcggat cgccagcgag atcgtccatc ccaatatcgc
     3421 ccaggtcttc gatttgggag agcaagacgg cgttttgttt ttggtatgcg agtgggtgga
     3481 tggtgaatcg ttggcaacgc tgcgcaaacg ggtggtggat caagggcaag aaatccccat
     3541 accgattgca cttcggatca tgtccgatgt ctgcggagga ttgcacgccg cccatgaact
     3601 ccgcgatgtc acaggcaaac ctttgcacgt cgtgcatcgc gatgtatccc cgcaaaacat
     3661 cgtgatcagc gatgctggcg cggtcaaagt catcgatttt ggagtcgcca aagcgcaaaa
     3721 ccgaaagctg gatcaaaccg tcgccggaaa gctgaaaggc aaagtgcagt acatgccacc
     3781 ggagcaagcg cggggcttgc ccgtggatag aagaacggat ctgtggagcg taggagctgt
     3841 tttgtaccat cttctcgcgg gaagaacgcc gtacgaagcg gactcgccgc tcgccgtgct
     3901 caacttattg ctgtcggagg aagctccacc acctttgcca gcccatgtgc ccgaaagcgt
     3961 tgctgatatc gtattccaag ccttgcgccc caaccccatc gaacgctttc aaaccgctgc
     4021 cgagatgcaa atcgccattg cgatggcgct gcgcaacgta tccatgccga tccccgatgc
     4081 ggccatcgga gagttcgtgc gcaggtatgt cggtgctcaa gccaagcagc ggcatcaact
     4141 catcgctgaa gccatcgaag ctgctaacca cgctcctcct ccccccgccg acctcaccct
     4201 cgtggaagtc gtggaagcgg tagaagaact gatttcaaaa gaaaaagagc cttcgtcacc
     4261 cgcgtccccc cccgaagccg ttaggccgcc accccacgaa aaggttggac agactctcgt
     4321 cgcaccgttt cccgctcccg ctcagccgcc catcgaaccc gaaaacgaag aacccgtact
     4381 gttggtgcgt cgctctcgcg cggattggac tccgacttcc gctcccacgg tggtggaaca
     4441 ccccgtggtg aacgaacctt acccgatggc acaggacaac cgcgcgccat ccctagcgcc
     4501 atccgcagcg ccatccccag cgcaatctcc gccgcgaccc acacccaatc cttccaccac
     4561 ggccgtcacc gatccggcga tcacgggccc tcggcgcgcc gtggccttgc ccgcggtccc
     4621 tcaaatcctc gtagctgtgg tcgtgtttct tgccgtcatc gcgacctttg ccttgttctg
     4681 gccgaagacc aagccaccgc tgggcagctc ccctgccctt tccgcttccg aaacagcgaa
     4741 agccgtattg ccccaatcgt ccgcggaacc gaatccctcc gagcatgacg atatccccat
     4801 cgtatcgggt tcggatttgc ccacggtcgc cccttcaatc cctgactctt cccgactgcg
     4861 tccgaaacgc ccccttccgg gatccacggt tccaccaccg cctcccgggt gggaatcgct
     4921 gccttccctg acgttggagc ccgaatcgcc gtaaattggc tataacccca cgctcttccc
     4981 tcttcgcttt cgaacctcgg gccaagaaaa catatcccca attgatcgtt ccccaaccag
     5041 ctcgtggtag atcttgctcg atatgggatc caccccaacc cgttggcccc tgctgctcac
     5101 tgccacgctg atcgtcgtgc tgaccgtcgc ggtctatttc ccttccttga agaacggtag
     5161 cgtctgggac gatttcgatt tagtaacgga aaatccatat cttagatcat tttcgggaat
     5221 ggcgaccttg ttcgagacgg acctctggac tgcctccgcc aagcgtgagc caagcagttt
     5281 ttatcgtccc ctcacgatgg tgacgttctg gatcaacgca tggatagccg gtccgtcggc
     5341 aacttccatg cgagcgggca acgtgatggt ccatgccacc aacgctctgc ttttatggct
     5401 gctgttgcgt cgacttctat ctccaacccg cactcttctt tccgcttcga tagcggggct
     5461 gtgggccgtg atggcggtca atagcgagcc ggtgttgtgg atatccggca gattcgatcc
     5521 tctgacggtg acggtcgcgc ttgggtgcat gcttgcgaat cgactccgca gcgtttgggg
     5581 gacggttttc gtggtgctgc tggcgacttg cgggcttttc atcaaagaga cgttcatggg
     5641 gtggcttccc ctgttgtttt tggacgacgt atggctgctg gggcgtcatc cgaagcagag
     5701 tattggaaaa gcgattgggt tggcggtggt cattggttt
//
LOCUS       DAOSOR010000384         5733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14187_length_5733_cov_4.195315, whole genome shotgun sequence.
ACCESSION   DAOSOR010000384 DAOSOR010000000
VERSION     DAOSOR010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5733
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14187_length_5733_cov_4.195315"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            450..1217
                     /locus_tag="PK710_17755"
     CDS             450..1217
                     /locus_tag="PK710_17755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11627.1"
                     /translation="MGISFEKFFAVTALMAGVGLVVACTVEENDDEGAAAAAGSGGSG
                     QAGEAGTGGGSAGEAGSAGEAGSAGVAGGAGEGGSGGVAGGAGEGGSGGTPQCLGDTI
                     EGDPALEGICDDLPYAETDCDPGSEAGLEPYAYEVCWRMSGRLRDAVLYDMHTCLADI
                     DITDACSDEHGDAAEKCMVASVGNACELSEAAGTCDAIKTACDAVDANLCVALMQAFK
                     PAAHQEILDCIDMGKKASTDPCEDIVDLCIWDPWAFY"
     gene            1370..2674
                     /locus_tag="PK710_17760"
     CDS             1370..2674
                     /locus_tag="PK710_17760"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOT11628.1"
                     /translation="MSPSIVHRPITYDEAEALEQAAAGRIETTRPTPPPYPWADSFFT
                     VGVTGTNGKTSTTLLLTSIFRAAGHSVLTETTLGYSLDDKVLQVPRTTQGFLTAMREA
                     AMAGARHAVIETTSQALARGYAKMWRFDLGVFTNLSQDHLAVHGSWEHYLASKAQLFV
                     HLGPGRTAVLNAFDPACVLIDKILPTDILRLWVGVASRGQPHHEPDLTASNVEVSSRG
                     TRVSLQPSPLAEQLGSMLEIPLVGEVFAENALCAAAAAHGAGCCAQAIRQGLRECPPI
                     AGRFEIVHHDPIVVVDFAHTPDALVRTLDTARRLTSQRVFAVFGAGGERDRDKRKPMG
                     RAVGQRADMAVVTNDNPRHEDPSSIARQVAAGCRQGGRAYVRIELDRKKAIITALQEA
                     RQGDVVVVAGKGHEQGQIIGDRTLPFSDGQVVRDFFERSAMD"
     gene            3006..3944
                     /gene="mdh"
                     /locus_tag="PK710_17765"
     CDS             3006..3944
                     /gene="mdh"
                     /locus_tag="PK710_17765"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOT11629.1"
                     /translation="MARKKIGLIGAGNIGGELANQIVEKQLGDVVLVDIPEKEGVAKG
                     KALDIMQNSALIGKDASCIGTSNWQDVAGADVIIVTAGIPRKPGQSRDDLVGVNVGII
                     RTVADNAKKYCPNAFAIIVANPLDAMVFEFHRRSGFDKSKVCGMAGMLDSARFQYFLA
                     DAANVSVKDVRTMVLGGHGDDMVPISSMCTINAVPASELVASDKMAAIIQRVRGGGGE
                     IVKLMGTSAYYAPASAAIQMAESFLFDQKRLMPCAAYLTGQYGYKDMFIGVPIIIGAS
                     GVEKVIEVPLTVDETAALKKSAEAVMTVVDIVKKTP"
     gene            4095..4988
                     /locus_tag="PK710_17770"
     CDS             4095..4988
                     /locus_tag="PK710_17770"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HOT11630.1"
                     /translation="MPIGISSLLKAPVVYVTGKGGVGKTTVAAAIAYDASRHGQRAAL
                     VEFDDGEAGERTLGADRSHVAHVVLRYDKSLVDTLGSILGAKIIARALVSHHALRRLI
                     SAVPALREFAFLDRVCALASSKRYDRIIVDLPASGHALDWLRVPNAFARFLGRSPLGV
                     LGRRIHDEVVAVGMSEIVVVTLAEPLVVKETEQLCERMKNELGTVPSVVVVNRVMMPD
                     PDGAWEAVVALAERHPALASDAKELLDILRARSDQVDDTEYAFGLARAVQARHVIRLP
                     DAAADPSAADVAQWLCAEASS"
     gene            4985..>5733
                     /locus_tag="PK710_17775"
     CDS             4985..>5733
                     /locus_tag="PK710_17775"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HOT11631.1"
                     /translation="MSPIDMSPEGAGPASRPAPPPMGTRLSVCVGGGGVGKTTTSAAL
                     ALSLAQRKRKTLVITVDPARRLADALGTEVGRHAQPVTLDRAGGMLFARMPDSRMSLD
                     DFVLWLFEDPVQRQRVMNNPAYRELGDSLAGVHELITIGLLQTEIDSGHWDEVVLDTA
                     PSRHALAFLTYPSRLLGLLEAKALAWLAALASTADPSQAEHKPKSGLFEWGRSKAEAI
                     MGKIVGLDGLRNLSALFADMVSVRERWAGLAR"
BASE COUNT         1171 a         1593 c         1742 g         1227 t
ORIGIN      
        1 cgattacaac gtaatctggc gcttgttcct ctcccgtttg cgaaatctac gttcagtgtt
       61 acgcaaagca tagcgggcca acgtggggca tcgaatgaga tcgcgacgga cgatacatgg
      121 ctgctttgcg ccgctcagcg gtagtgcctc acagtcgtcg gaaatagccg tttttgtatt
      181 caatcaagaa tctcgcgacg atgggaagaa gcgttaagtc ccttttgccc cgataaacag
      241 cttctcaatc ggaagaggca agcttttcat ccgagcccag ggtaaggata tgggagcggg
      301 tgataatctt tgtctttcga gggaagtttg acaggggtgt ttgcgtatcg taaaccataa
      361 ggtatcacga attttcggtt cgcagcaatt caaccatcag aaaacacggc tgatggtgct
      421 taggcgggag accgctacag gaggatatga tgggaattag ctttgaaaaa ttctttgcag
      481 tgacagctct catggcgggc gttggccttg tcgtggcatg cacggtcgaa gaaaacgatg
      541 atgaaggagc ggcggccgcg gctggttccg gaggaagtgg acaggccggt gaagcaggga
      601 caggtggtgg tagtgctggt gaagcgggca gcgcgggcga ggcgggctcc gcgggtgttg
      661 ctggcggcgc tggtgaaggc ggctccggcg gtgttgctgg cggcgcgggt gaaggcggct
      721 ctggcggcac ccctcaatgc ctcggagata ccatcgaagg tgacccggcc ctcgaaggaa
      781 tttgtgacga tctgccctat gccgaaacgg attgtgaccc cggttccgaa gccggcctcg
      841 agccctacgc ctacgaggtc tgctggcgaa tgtccggacg cctccgcgac gccgtcttgt
      901 atgacatgca tacatgcctg gcggatatcg acatcactga tgcttgctcc gatgaacatg
      961 gtgacgcagc cgaaaaatgc atggttgcct cggtcggtaa tgcttgcgaa ctttccgaag
     1021 ccgccgggac ttgcgatgcg atcaagaccg cttgtgatgc tgtcgacgcc aacttgtgtg
     1081 tcgctttgat gcaagcgttc aaacctgccg cccatcaaga gatcctcgac tgcatcgata
     1141 tggggaaaaa agcgagtacg gatccttgcg aagatatcgt ggatctctgc atctgggatc
     1201 cgtgggcttt ttattgatac ccgaaacctc cgtcctttta taactcctcg taatagttga
     1261 ttatttatct aacggctccc tgcggaggct gcttccggag tgcggctatc tggctggcac
     1321 gctgctcggg acgcgctcct tgctatcgtt gggtgctagc atacccggca tgtcgccgag
     1381 cattgtgcac cgtcccatca cctacgacga agctgaggcc ctcgaacagg ctgcagccgg
     1441 gcgaatcgaa accacccgtc ccacgccacc tccgtatcct tgggccgatt ctttcttcac
     1501 cgtcggagtc acgggcacga atggcaagac ctccacgacg cttttgctga cgagcatttt
     1561 tcgcgcggcg ggacattcgg tcctgacgga aacgacgttg ggatattcac tcgatgataa
     1621 ggttttgcag gtgccgcgaa ccacccaagg atttttgacg gccatgcggg aggcggcgat
     1681 ggcgggggcg cgtcatgcgg tgattgagac gaccagtcaa gcgttggcgc gaggatacgc
     1741 gaagatgtgg cgtttcgacc tgggggtgtt caccaattta tcgcaagacc acctcgcggt
     1801 gcatggttcc tgggagcatt atttggcgag caaagcccag cttttcgtgc atcttggacc
     1861 gggacggacg gctgtgctca acgcattcga tcccgcctgt gtactgatcg acaaaatcct
     1921 tcctaccgat atccttcgat tgtgggtagg ggttgcgtcg cggggccaac cgcatcatga
     1981 gcccgacctg accgcttcga acgtggaagt atcctcgcgg gggactcgcg tgtcattgca
     2041 accaagccct cttgccgagc agttgggaag catgctggag attccgctgg tgggggaggt
     2101 ttttgccgag aatgcattgt gcgccgcggc ggctgcccat ggggcaggtt gttgcgcgca
     2161 agccattcga caaggtcttc gagagtgtcc tccgattgcc ggacgtttcg aaatcgtgca
     2221 tcacgacccc attgtggtcg ttgattttgc ccatacgccc gacgctttgg tgcgcaccct
     2281 ggacacggct cgccgactca cttcccaacg agtattcgcg gtgtttggcg ctgggggcga
     2341 acgcgatcgg gacaagcgaa aaccgatggg acgcgctgtc ggacagcggg ccgatatggc
     2401 cgtggtgacc aacgataatc ctcggcatga agacccttcg agcatcgcgc gacaggtggc
     2461 cgccggatgt cgacaagggg gaagggcgta cgtacgaatc gaattggacc gaaaaaaggc
     2521 gattatcacc gcgttgcaag aggcgcgaca gggagacgtg gtcgtggtgg cgggaaaagg
     2581 gcatgaacaa ggccagatca ttggcgatcg aacgctgcct ttcagcgatg ggcaggtggt
     2641 gcgcgatttt tttgagcgat ctgccatgga ctgaacggaa caatgatttc acgaaattat
     2701 ccggatacgg atagcgtgaa aagggcctgc gaaccattcg aatacggtcg ttgggggaga
     2761 gaacccccag cagcggttga ggtaagcaaa ggaaatcttg cgattcacga tttcgatttc
     2821 gtgacgacgc ttgcctcccg tgagcgggtg gagtagctca ccccatgggt caatgcaaag
     2881 ggccaacagg cctggcgcga ccgctggtca ctggaagctt gggcaatggg gcttgtcgcc
     2941 cgttgttcca tgcaccggtg gcgtagatat cgtagcccct ggcaatgggg aaggagagca
     3001 agaacatggc acgcaagaag atcggtctca tcggcgcagg aaatatcggt ggtgaactcg
     3061 cgaaccagat cgtcgaaaag cagctcggtg acgtcgtgct cgtcgatatc cccgaaaaag
     3121 agggggtagc gaaaggcaaa gccctcgaca tcatgcaaaa tagcgcgctg attgggaagg
     3181 atgcgtcgtg catcggcacg tccaactggc aagatgtggc cggcgcggat gtgatcatcg
     3241 tcaccgctgg catcccgagg aaaccagggc aaagccgcga tgacttggtc ggtgtgaacg
     3301 ttgggattat tcgcacggtt gcagacaatg ccaaaaaata ttgccccaat gctttcgcca
     3361 tcattgtggc caaccccctc gacgccatgg tgttcgagtt ccatcgtcgc agcggcttcg
     3421 acaagtccaa ggtctgcggc atggcgggca tgctcgatag cgcgcgattc cagtactttt
     3481 tggcggatgc ggcaaacgta tccgtgaaag atgttcgcac gatggtgctt ggcggtcatg
     3541 gcgacgacat ggttccgatc tcttcgatgt gtaccatcaa tgccgtaccg gcttcggaac
     3601 tggtggcttc cgacaagatg gccgccatca ttcagcgagt tcgcggtggt ggtggcgaga
     3661 tcgtgaaatt gatgggaacc agcgcctatt atgcgccggc ctccgcggcg attcagatgg
     3721 ctgaatcttt ccttttcgac cagaagcgcc tcatgccttg cgccgcgtac ttgaccggac
     3781 agtacggcta caaggatatg ttcatcggcg tgccgatcat catcggtgcc agcggtgtgg
     3841 aaaaagtgat cgaggtgcct ctgacggtcg atgaaacggc ggccctcaaa aagagcgccg
     3901 aagcggtcat gaccgtcgtc gacatcgtga agaaaactcc ctgatccctt cgcccggctt
     3961 cagcctgccg tggctattca atcatcgaaa gcttgacttt ccgcctcacc cgctcttaat
     4021 cccatcgtct ccctgtcggc ttcgccttgt cgagccgcaa gaacccaacg agaaggaagc
     4081 tccccttgac cacgatgccc atcgggattt cgtccctgct gaaggccccc gtggtctacg
     4141 tcaccggaaa aggcggtgtc ggaaaaacaa ctgtcgccgc cgccatcgcc tatgacgcga
     4201 gccgacacgg ccaacgcgcc gccttggtcg aattcgatga cggagaagca ggcgaaagaa
     4261 ctctcggcgc agaccgctct catgtggccc atgtcgtgct gcgctacgat aaatccctcg
     4321 tcgataccct cggttccatt ctcggcgcca aaatcatcgc gcgagcgctc gtctctcatc
     4381 acgccttgcg acgtctcatc agtgccgtgc ccgcccttcg agagtttgct tttctcgatc
     4441 gcgtttgtgc cctcgcgagt tcgaaacgtt acgaccgcat catcgtcgac ttgcccgcga
     4501 gcggtcatgc cctcgattgg ttgcgtgtcc ccaatgcctt cgctcgtttc ctcggtcgaa
     4561 gcccgttggg cgtgctgggc cgaagaattc acgacgaagt cgttgccgtg ggaatgtccg
     4621 aaatcgtggt ggtgacgttg gccgaaccgc tggtcgtcaa agaaaccgag cagctttgcg
     4681 aacgaatgaa aaatgaattg ggaaccgttc catcggttgt cgttgtcaat cgtgtgatga
     4741 tgcccgatcc ggatggagct tgggaagccg ttgtcgcgct tgcggagcga catcccgcgt
     4801 tggcctccga tgcaaaagaa ttgctcgaca ttcttcgcgc ccgaagcgat caggtggatg
     4861 atacggagta cgcattcgga cttgcgcggg cggtgcaagc gcgccacgtc attcggcttc
     4921 cagacgcggc ggccgatccg agtgccgctg atgtcgccca atggctttgc gcggaggctt
     4981 cgtcatgagt ccgatcgaca tgagccccga aggcgcaggt cccgcctctc gacccgctcc
     5041 tccacccatg ggtacgcgtc tatccgtttg cgtgggcgga ggcggtgtcg gcaaaaccac
     5101 tacctcggcc gcgttggctc tgagcctggc ccaacgaaaa cgaaaaaccc tcgtcattac
     5161 cgtcgacccc gcgcgccgtc tcgccgatgc ccttggcacc gaagtgggaa gacacgcgca
     5221 gcccgtaaca ctcgatcgcg cgggcggaat gctcttcgcc cgtatgccgg actctcggat
     5281 gtccctcgac gatttcgttc tctggttgtt cgaagacccc gtgcagcgtc aacgggtgat
     5341 gaataaccca gcctaccggg aattgggtga ctctctcgcg ggcgttcatg aactcatcac
     5401 catcggattg cttcagaccg agattgatag cggtcattgg gatgaagtgg tcctggatac
     5461 cgcgccgtcg cgacacgccc tcgcgttttt gacgtatccc tcccgcttgc tgggattgct
     5521 ggaagccaag gctttggctt ggctcgccgc cctggcttcg accgccgatc cttctcaagc
     5581 agaacacaaa ccgaaaagtg gattgttcga atgggggcgc tccaaagcag aagccatcat
     5641 gggaaagatc gtcggcctcg atggtctgcg caacttatcc gctctgttcg ccgacatggt
     5701 ctccgtgcga gaacgatggg ccggactcgc gcg
//
LOCUS       DAOSOR010000385         5728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14208_length_5728_cov_4.435748, whole genome shotgun sequence.
ACCESSION   DAOSOR010000385 DAOSOR010000000
VERSION     DAOSOR010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14208_length_5728_cov_4.435748"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PK710_17780"
     CDS             complement(<1..614)
                     /locus_tag="PK710_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT11632.1"
                     /translation="MQDVRALNELVAKHSDFTESLIAEVGKVIVGQTYMVERILIGLL
                     ASGHVLLEGVPGLAKTMSVKTVCEALSGKFARVQFTPDLLPADLTGTVVYNHHKGEFS
                     SKLGPIFANFVLADEVNRAPAKVQSALLEAMQERQVTLGDTTYDLPDPFLVMATQNPI
                     EQEGTYPLPEAQVDRFMLMVRVGYPSVEEERLIIDRVTAPIPATV"
     gene            complement(768..2228)
                     /locus_tag="PK710_17785"
     CDS             complement(768..2228)
                     /locus_tag="PK710_17785"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT11633.1"
                     /translation="MTARDTMTGQPLQIVLVTKAWTTAEVYSRALTSAGHTVSTATST
                     AEALAVLRRDGCDVLVTDARVERDDDGVQLAKEVLNKSKGVQVIVLAERPNVQDAVML
                     TKLGAYDYQATPVSPDKLRTMVGSIADIFSTHQEETGIDDFLRAFMVSSVPAVQEMVV
                     QCRTAATRPDTTALIVGEPGAGKGIVSRMIHSLSAQSKGPFVRLNLVDSDPSDIEQKL
                     FGTDTGDDGLLAAASGGTLLLREFSDIQFDLQERMRELLETRSYYPVQGFTRRHFNGR
                     LLATSSLSVGELVSDAMVDAKLAYRLATVTIHVPPLRERNDDLLRIADAMLAEIAMDA
                     GRPQLVVTEEARQLLKDHPWLGNLREMRNVLTRLVLLSPSDEVDADTLARALGFPSAE
                     ARRLHKSGLRNRVRTTTRRPRNSSVPVPRLSGLLATALDEPARAERERIENALEATEG
                     HRERAAAVLGMSRTTLWTRMRMLGIDYERFRRSKAV"
     gene            2683..>5728
                     /locus_tag="PK710_17790"
     CDS             2683..>5728
                     /locus_tag="PK710_17790"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOT11634.1"
                     /translation="MPSPTPLIIHCPRWDRQRREIMVMPLGLPALANLILDHTGTAPT
                     LVHLGIENEHDPHFRLSELLSPSPPRLLLVSLHWFLQVRPVLDLVRELRSLAPESLVV
                     LGGFTASYFAHEILSRVPSVDAVVVGDGEQPILALTRWASAIPALSRSRLNEIPNLVW
                     RDTDGTIRRNPHRYVLGSSEAASLRHGSLSCLRHHQLYTERALYADFSEGSHESPYAR
                     TAYLNAGRGCSVACANCGGGKLAQAALGKRNGILVYPLSKLIRDIEESLSHGVDVLRM
                     SFDPPSARRPMMAWFEAIAKLNPRFRLIYDLWWLPSKALLDSMARAFSPGSTLILSPE
                     CGSEAVRKRIRGYPFSNDHLMQAIHAIESRGFRTHCFFSAGLPTESPDDLEETIGLIQ
                     RIRGETQAGISVCPMTADPASPLFTNPDAFGATLTRQSLLDFYHDPSPHRPGYRTAYF
                     SESQILDACNRLLQAAALPPSLDPIVDSSSSMSRNSLEIAPNPSKRVVLLVGMFRRIV
                     PEGNDGPFGSQGGFVDNLAPSPYSLANGYLKSFVDANDALRERYDVRLFDIIEPWEPE
                     EEREEVRITDEDIDALLSYHPVLIGFSAYCWNLDAVRTACQTIKQRQPDVRIVVGGRV
                     SQSDARDWLASCPAVDAVVVGEGELAFREILLRDGFSGIAGVLWREGNELRWGGAPRC
                     LDSLDDIPSPFLTGVLVPALHAVMLELSRGCLHACGYCTWNADKRLRHFGSSRIEQEL
                     RWAVRQGHQHITLNDSAINYDTERLRDFVEAIERADPSNRLRFTYNFRHDALTEEQFE
                     LFQRLPTHVALVGVETLSSRGMAQVDRQAVDVPALRYRLERFARTVRPAVASIVLGLP
                     GDDEQGFLDTLDTLMSYTQPTDGPPFVEAVLVSLLQVYRGSKLWQRREQLGLVFNPRG
                     IPYLIESASWTKNALARCKTAIIRRIRQNPSRLKAAEALVMMREQGGLPVWLSPRVLA
                     IVLRDWPEGKTYEGWTLERIGRMRDTGQGALLRFRFQSKGCVR"
BASE COUNT         1180 a         1703 c         1540 g         1305 t
ORIGIN      
        1 acggtggctg gaatgggagc ggtaacccga tcgatgatca gacgctcttc ttcgacggat
       61 ggataaccaa cgcgaaccat gagcatgaaa cggtcgacct gagcttcggg aagaggatac
      121 gtaccttcct gttcgatggg gttttgggta gccatgacga ggaaagggtc gggcaagtcg
      181 taggtggtgt ctcctaacgt tacttgacgc tcttgcatgg cttcgagcag ggcgctttgg
      241 actttggcag gggcgcggtt gacttcgtca gcgagcacga agttggcgaa aatggggccg
      301 agcttcgagg aaaactcgcc tttgtggtgg ttgtagacga ccgttcccgt caggtccgcg
      361 ggcaataagt ctggcgtgaa ctgcacgcgc gcgaactttc cggataaggc ttcgcaaacc
      421 gttttgacgc tcatggtttt cgcgagccca ggaacgcctt cgagaagaac gtgcccggaa
      481 gcgagtaaac cgatgagaat tcgctcgacc atatacgttt gaccgacgat gaccttgccg
      541 acttcggcga tgagagattc ggtgaagtca ctgtgcttgg ccacgagctc attgagcgcg
      601 cgtacgtcct gcatgaacga gaacctccaa agcgaaaaaa aggatgaaca acccgggcgc
      661 catgtatagc cgctcggccg cacgagacaa tcgagagttg caggctattc ccaagaatcg
      721 gtcagcaggt cattatttcg acagaaatac ccttcgttgc ccaagggcta cacggccttg
      781 gagcgacgga atcgctcgta atcgatgcca agcatccgca tccgggtcca tagcgtggtc
      841 cttgacatgc cgagcactgc ggccgcccgc tcccgatgcc cctcggtagc ctcgagagcg
      901 ttttcgattc gctccctttc cgcccgtgcg ggttcatcga gcgccgtggc gagcaatccg
      961 gacaatcgcg gaactggaac cgaagagttt cgcggacgac gcgtcgtggt ccgcacccga
     1021 tttcgaaggc ccgatttgtg gagtcgtcgc gcttccgccg aaggaaaacc caaggcccgc
     1081 gccaacgtat ccgcatcgac ctcgtccgat ggactcaata gaacgagccg cgttagcacg
     1141 tttctcattt ctcgtaggtt gcccaaccat gggtggtctt tcaaaagctg ccgggcttct
     1201 tccgtgacga caagctgcgg ccttccggca tccatggcta tctcggcaag catcgcatcc
     1261 gctatccgaa gcaaatcatc gttgcgttcc cgcaaaggcg gcacgtgaat ggtgaccgta
     1321 gcaagacgat acgccagctt cgcatccacc atcgcatcac tcaccaattc ccccaccgac
     1381 aacgaactgg tggcgagcaa ccgtccgttg aaatgcctgc gcgtgaatcc ttgcaccgga
     1441 taataggatc gcgtttccag caactcccgc atacgttcct gcaaatcgaa ttgaatatcc
     1501 gagaattcgc gaagtaacaa agtaccaccg gatgccgccg caagaagacc gtcgtcgccg
     1561 gtatccgttc caaaaagctt ttgttcaata tccgaaggat cgctgtccac caaattgagc
     1621 cgcacgaagg gccccttcga ctgagcgctc aacgagtgaa tcatccgtga aacgatccct
     1681 ttgcccgcac caggctcccc cacgatgaga gcggtcgtgt ctggacgtgt tgccgccgtt
     1741 cggcattgga ccaccatttc ctgaacggct ggcaccgacg agaccataaa cgctctcagg
     1801 aagtcatcaa ttcccgtctc ctcttgatgt gtcgaaaaaa tatcggcaat cgacccaacc
     1861 attgtccgaa gtttgtcggg cgatacgggg gtggcctgat agtcatacgc cccaagtttg
     1921 gtgagcatga cggcatcttg tacattcggt cgctccgcca gcacgatgac ttgcaccccc
     1981 ttcgacttgt tcaacacttc tttagccagt tgaacgccat catcgtcgcg ctcgacacga
     2041 gcatccgtca ccaaaacgtc gcaaccgtct cttcgcaata ccgccagtgc ttccgccgtg
     2101 gacgtcgcgg tcgataccgt atgaccggcg gaggtcagag cgcgcgaata gacttccgca
     2161 gtcgtccaag ctttggttac caatacgatc tgaaggggct gtccggtcat ggtatccctc
     2221 gcggtcatgc aagttatggc ttcgattacg tcaaggttgc gttgccttta caaatatcgg
     2281 ccatcgtaaa aaatacctcg ccacggcctg actgacaaca gaataagatt tttatttgta
     2341 aatacaaatg gttatgtagg tgtcgccagg caaaagatgc caagaccctt ggctattcca
     2401 cgatttttgg ggacgttgtg atttcacaac gtgacgaaaa cgtcagcgaa gccaaaggct
     2461 tgtccgctgc cgcattcatt taattgtgtg taaaatcaat atattacacc aggcacagac
     2521 acgctgggcc cgacgctgaa catggcatgg agccgtggga gacacgcgaa gatcaggaaa
     2581 tgaacatcat ggacggcgtt cttgcgccat gatgccattg ccactgcttt tgcttccccc
     2641 ttttcttgcc tcccggcagc ccagtcccca agcttgtctc ccatgccctc tcccacgccc
     2701 ctcatcatcc attgtcctcg ttgggacagg caacgacgcg agatcatggt catgcccctt
     2761 ggcttgcccg ccctcgccaa cctcatcctc gaccatacgg gcaccgcgcc gacgcttgtc
     2821 cacctcggta tcgaaaatga gcatgacccg catttccgtc tttccgaatt gctgtctcct
     2881 tcgccccctc gcttgctgct cgtgtcgctc cattggttcc tccaagtccg tcccgtgctc
     2941 gatctcgttc gcgagttgcg aagcctcgct cccgaatcgc tcgttgtgtt gggcggcttc
     3001 acggcctcgt acttcgctca tgaaatcctg tcccgcgtgc caagcgtcga tgcggtcgtc
     3061 gtgggagatg gggaacaacc catcctggcg ttgacgcgct gggccagcgc cattcccgct
     3121 ttgtcacggt cgcggttgaa cgaaatcccg aacctcgtgt ggcgggatac ggatggtacg
     3181 attcgtcgca acccgcaccg gtacgttctt ggttcgtcgg aagctgccag cctccggcat
     3241 ggaagcctat cgtgcctgcg tcaccaccaa ctgtataccg agcgcgccct ctatgccgat
     3301 ttcagcgaag gaagccatga atccccgtac gctcgcaccg cctacctcaa cgccggaaga
     3361 ggatgctccg tagcctgcgc caactgcggt ggtggaaaac tcgcgcaagc ggctctgggc
     3421 aaacgaaatg gtattctcgt ctatcctttg tccaaactca tccgcgatat cgaggaaagc
     3481 ctgtctcacg gcgtcgacgt tctccgcatg tcttttgatc ctccgtccgc gagacgaccg
     3541 atgatggctt ggttcgaggc catcgccaaa ctcaatcctc ggtttcgcct catttatgac
     3601 ttatggtggc ttccttcgaa ggcgctgctc gattcgatgg cgcgagcttt ttctccgggg
     3661 tcgacactga ttctttcacc ggaatgcggc agcgaagccg tacgaaagcg gattcgcggt
     3721 tatcccttct cgaacgacca tctgatgcaa gcgattcatg ccatcgagtc ccgtggcttt
     3781 cgcacccatt gttttttttc cgccggtctt cccaccgaat cccccgacga tctcgaagaa
     3841 accatcggcc tcatccaacg aattcgaggc gaaacgcagg ctggaatttc ggtgtgtccc
     3901 atgaccgccg accccgcaag cccccttttc accaacccgg acgctttcgg cgccacgctt
     3961 acacgacaat cgctgcttga tttttatcac gatcccagcc cccatcgtcc aggttatcga
     4021 acagcgtatt tttccgagtc tcaaatcctc gatgcttgca accgactcct gcaagcggcc
     4081 gccttgccac cttccttgga ccccatcgtc gattcctcct cgtcgatgtc acgaaattca
     4141 ctggaaatcg ctcccaatcc atcaaagcgc gtcgtgttgc tcgtcggcat gttccggcgc
     4201 atcgttccag aaggaaatga tggtccgttc ggcagccagg gaggattcgt cgataacctc
     4261 gccccctccc cctacagcct cgccaatgga tatctcaagt ccttcgtcga tgcgaacgac
     4321 gcgcttcggg aacgatacga cgtgcgtctc ttcgatatta tcgaaccctg ggagccagag
     4381 gaagagcgcg aggaagtgcg tatcaccgac gaggacatcg atgcgcttct ttcctatcat
     4441 cccgttttaa ttgggttttc tgcttattgt tggaatctcg acgcggtccg gacggcttgt
     4501 caaacgatca agcagcggca accggatgtg cgtatcgtgg tgggtggaag agtctcgcaa
     4561 agcgatgcac gcgattggct tgcgtcctgt cccgccgtcg atgccgtggt cgttggcgaa
     4621 ggggaactgg cttttcgaga aatcctgctt cgagatggct tttccgggat cgcgggggtg
     4681 ttatggcggg aaggcaacga gttgcgttgg ggaggagccc ctcgatgctt ggactctctc
     4741 gacgatattc cttccccttt tctcaccggt gttctcgttc ctgccctcca cgccgtgatg
     4801 ctcgaattgt ccagggggtg tttgcatgct tgcggctatt gcacgtggaa tgcagacaaa
     4861 cgtctccggc attttggctc ctcgcgtatc gaacaggagc tgcgttgggc cgtgcgacaa
     4921 ggacatcagc atattaccct gaacgattcc gccatcaact acgatacgga gcggcttcgc
     4981 gatttcgtcg aggcaattga gcgtgcggat ccctcgaatc gccttcgttt cacgtataac
     5041 tttcgacacg atgcgttgac ggaggagcaa ttcgagcttt tccagcggtt gcccacccac
     5101 gtagctctcg tgggtgtcga aaccttgtcg tcgcggggca tggcccaggt tgatcggcaa
     5161 gcggtggatg ttccagccct tcgatatcgc ttggaacggt ttgcaaggac cgttcggccc
     5221 gcggtggcca gcatcgtgtt gggactgccg ggcgatgatg aacagggatt cctcgacacg
     5281 ctcgatacct tgatgtctta tacgcagccc accgatggtc ctcctttcgt cgaagcggtg
     5341 ctcgtatcgc tgctccaagt gtatcggggt tcgaaactct ggcagcgccg cgaacaactc
     5401 ggacttgtct tcaatccgcg gggaatcccg tacttgatcg aaagtgcgtc ctggacaaaa
     5461 aatgcgctgg cgcgttgcaa aacagcgatc attcgtcgga ttcgccaaaa cccttcccgc
     5521 ctaaaagccg ctgaggcact cgtcatgatg cgtgaacagg gaggattgcc cgtctggctt
     5581 tctccccgcg tgctcgctat cgtattgcgc gactggccgg aaggaaaaac ctacgaggga
     5641 tggacattgg aacggatagg acgaatgcga gatacggggc agggagcctt gttgcgcttt
     5701 cgttttcaat ccaaaggctg cgttcggg
//
LOCUS       DAOSOR010000386         5659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14438_length_5659_cov_4.615810, whole genome shotgun sequence.
ACCESSION   DAOSOR010000386 DAOSOR010000000
VERSION     DAOSOR010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14438_length_5659_cov_4.615810"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..1078
                     /locus_tag="PK710_17795"
     CDS             236..1078
                     /locus_tag="PK710_17795"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOT11635.1"
                     /translation="MRWLMVLGLIVTGAVSCRPVEKRASPSISEIQSAVSVSPPLHSI
                     ALPSNVATISNTKIASFGKAKKHLWEIHKDHQTTLYCGCAYEGNELVSPPGACGYVPI
                     TTGIRSRRVEWEHVVPAQAFGQSFREWREGHPECVDGRGKPFRGRPCARKVAVAFRYM
                     EADMYNLQPAIGDVNDRRGTATPAMIPGENRLFGTCDFEVSAGKFEPRPEVRGDIART
                     YLYMDMAYPGRGILSEKNKKLFEAWSDQDPVDAWECERARRIARIQGNINQVVERACR
                     QAHR"
     gene            complement(1106..2008)
                     /locus_tag="PK710_17800"
     CDS             complement(1106..2008)
                     /locus_tag="PK710_17800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT11636.1"
                     /translation="MAKRSRQASKRTQKIRSSKRPLGSESVPTPKVKPEEQTPCETRQ
                     DAQDAQTPSFRERPTLAEPWVADARLDDRVLAPTHDELDEEFFRRADELELEYSRDLD
                     SYPPVVEPIVPPPEPSPDVLARRKKYRKLVAVVVTLGTLFSLGGVGKSMFDRPVAAAE
                     SPGGEQAKVVEKAAVDSQPIEIKTVSSEVPSSIPTPSAQSEASDKPTEAVAAPDKEAG
                     DYGDNPRATALSLLNRGKMKEAIPAARAAIEKEPEHALAYLYLGTALQDTGQHKTAME
                     VYSACVRTAKKGPVWECRAMGGKP"
     gene            2300..3118
                     /locus_tag="PK710_17805"
     CDS             2300..3118
                     /locus_tag="PK710_17805"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOT11637.1"
                     /translation="MDDHRKTKAQLVQEVKELRARVAAMAPQLAGTWVIDADFVVRVA
                     DQTMCRLLQKNATEVVGRKCYDLLDGPSCHSEACVLRQAFSKASGDVEALRTTVQQVP
                     GSCRIMASLIMNRDKEHVGVVQQVNHDTLPTCKPRHPRLRKVRSWRVAYLVCSQESDC
                     STQWSKTFRQAGFAVECQQATACLPPTRCPDVAVLDLDSLGRTSDAVHQKIRNACPQT
                     PIIVVCSKDDALEARRWKRTATWFVPKAVGADGILRLLEELQPKVGTARRKRSE"
     gene            complement(3399..4217)
                     /locus_tag="PK710_17810"
     CDS             complement(3399..4217)
                     /locus_tag="PK710_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT11638.1"
                     /translation="MKQKGLARYLLNAVLLIVAVAWLGPYLWMVLGSFKSLKDIMNAP
                     LAPLPSAFHGEAYAEVFRSIPVGRYLLNTTVMALSIAALQIMLVLPAGYALAKLRFVG
                     RGAAFLTVMACLLIPAQVTFVPVFLMLAQVKLVNTMTALVLPFGASALGTFMVRQALL
                     AIPDELIEAARLDGASELTIVYRILAPMLAPTLASLFLLSFVFHWNDYFWPLVMTTDE
                     SVRTMPLAVALLREQGTGVRWHIVMAGNVILSAPALFVFAMTQKHLLRAVAARR"
     gene            complement(4214..5086)
                     /locus_tag="PK710_17815"
     CDS             complement(4214..5086)
                     /locus_tag="PK710_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT11639.1"
                     /translation="MLRPRHRLHPYLFLAPTLGILGTFFLFPLGLAAYRSLYRWDLLT
                     PPRFVGLAHYERLFSSGEILRTLTITLSYAAVVVSGSMFLGLLLALAVNRKGKFVAFV
                     RSCVFSAYVVSWVSVALLWMWLLDADAGLVGRACKALGIGEAGLLTSPSTAIYALAGV
                     TIWKITGFSMVVFLAGRQDIPEDLYEAASLDGAGPLARFVHITWPCLRPTTAFVGTTS
                     LIVSFQAFDIVRVMTQGGPTHSTTVFVYAIYEHVLLNLRAGRASAMVVVFFGVLLLLT
                     LVQLWAWRMGRRSR"
     gene            complement(5093..>5659)
                     /locus_tag="PK710_17820"
     CDS             complement(5093..>5659)
                     /locus_tag="PK710_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT11640.1"
                     /translation="RMVHEDKTMRPPPGRDYNAWQVTNQDFLAGRAAMIYTSTAFLRY
                     LEEHATFPVRAAPLPGFRRRAVPTGGTFFVMMKSAPDHEKQAAWDFLRWMMQPEQTIE
                     WATSTGYMPVASDAVARLEREGFYQKSPNDRVAMDQLKYAMPWPWAESLFRVQRECVD
                     PRLEEAVLNRQDARSLLNEARKQALEDT"
BASE COUNT         1318 a         1611 c         1604 g         1126 t
ORIGIN      
        1 gtttttcaaa tatatcccag actcggaaga gccatggctg gcttcccgta tgcccaatcc
       61 ccaccgccgg cccccctgcc ctacctagac gtttcgttcc ccgctcacgg atggcaaggc
      121 tctaggccac tgttgctctt tcccatcccc gccccgatct gccatcgaag cgatggaaaa
      181 ccatcgttcc ttgatcttcg cctgcggatt tgcagtttta tgctagggtt gccgcatgcg
      241 ctggctcatg gtccttggat tgatcgtcac cggagccgtt tcgtgtcgtc cggtggagaa
      301 acgggcctcc ccatccatct ctgagattca gtccgccgtt tccgtttccc ctcccctcca
      361 ctcgatcgca ttgccgtcga acgtggcgac gatatcgaac accaaaatcg cctcgtttgg
      421 gaaagccaaa aaacacttat gggaaatcca taaggaccat caaaccacgc tatattgtgg
      481 ttgcgcttat gaaggaaacg aactcgtatc cccgccgggg gcctgcggat atgttcccat
      541 cacgactgga attcgatccc gaagggtgga atgggaacat gtggttcccg cacaagcgtt
      601 tgggcaatca tttcgcgaat ggcgggaagg acatcccgag tgtgtcgatg gtcgaggtaa
      661 gccgtttcga ggtcgtcctt gcgcaagaaa agtggcggta gcattccgat atatggaagc
      721 cgatatgtac aacctccagc ccgccatcgg cgacgtcaat gatcgtcggg ggacggccac
      781 acccgcgatg attcctgggg aaaatcggct atttggaacg tgcgacttcg aggtatcggc
      841 aggcaagttc gagccccgac ccgaggtccg tggagatatc gcccgaacct atctatatat
      901 ggacatggct tatccgggcc gaggcatcct gtcggaaaag aacaaaaaac tgttcgaagc
      961 ctggagtgac caagaccccg tggatgcctg ggaatgcgag agggcccgtc gaatcgcacg
     1021 gattcagggc aatatcaatc aagtcgtgga gcgggcttgc cgtcaagccc atcggtgatg
     1081 gcttcgagcc aaaaacaagt cgaaatcagg gttttccccc catggcccgg cattcccaca
     1141 caggaccctt tttggccgtg cgaacacacg cgctataaac ctccatggcc gttttgtgct
     1201 gtcccgtatc ttgcagcgcc gtgccgagat ataggtaggc aagagcatgt tcgggctctt
     1261 tttcaatggc cgcgcgagcc gcgggaatcg cttctttcat cttccctcga ttgagcaggg
     1321 atagcgccgt ggctcgggga ttatcaccat aatcaccagc ctccttatcg ggggcggcaa
     1381 ccgcctcggt cggtttatcg gacgcctccg actgcgccga aggcgtggga atggaactgg
     1441 gaacttcgga agaaacagtt tttatttcaa taggttgtga atcaactgcc gctttctcga
     1501 ccaccttggc ttgttcccca ccaggggatt cagccgcggc aacgggacgg tcgaacatgc
     1561 tttttcctac gccccccaaa ctgaaaaggg tccctagggt caccacgaca gccacgagtt
     1621 ttcggtactt ttttcgacgg gcgagcacgt cgggggaagg ttccggcggt ggaacgatag
     1681 gctcgacgac cgggggatag gaatcgaggt ctctggagta ttcgagttcc aattcatccg
     1741 ctcgtcgaaa gaactcctca tccaattcgt catgggttgg cgcgagcacc cgatcgtcca
     1801 agcgggcgtc ggcaacccac ggctccgcga gggtcggacg ctcccgaaaa ctcggcgttt
     1861 gtgcgtcttg cgcatcctgg cgcgtttcac aaggcgtttg ttcctccggc ttgactttgg
     1921 gagtgggcac cgattcagag ccaaggggcc ttttggaaga gcgtattttc tgcgttcgtt
     1981 tgcttgcctg tcgacttctt ttcgccatga cgacacctga gcctgcatcg gcggtaacga
     2041 tctctgatca aactcacttc aagatccggt ccatgctcaa agtcatagtt ttcggtagtt
     2101 gctactacag aaaagatggc acaccatgcc aatttgggtc acccgcggcc atggcctgtc
     2161 atcgtgatag tccactgaac gaattgacgg gcaggcagca agagcgcgga aggcgttctc
     2221 cccaaaaaat gttgcacact gctggttgta gcgatgtgcc agcaatgcta ccgaagaaag
     2281 aagattcggg aacgcaaccg tggacgatca caggaagacg aaagcgcagt tggtgcagga
     2341 agtgaaagag ctacgcgcgc gggtggcggc catggctccc caacttgccg gcacgtgggt
     2401 gattgatgcg gacttcgtgg tgcgcgttgc ggatcaaacc atgtgccgcc tgctccaaaa
     2461 aaacgccacc gaggtggtag gacgcaagtg ctacgacttg ctcgatggcc ccagttgtca
     2521 ttccgaagcc tgcgtgttgc gacaagcttt ttccaaagcc tctggcgacg tggaagccct
     2581 gcgaacgacg gtgcaacaag taccgggaag ctgccgaatc atggcgagcc tcatcatgaa
     2641 ccgcgacaaa gaacacgtgg gcgtcgttca gcaagtcaac cacgacacct tgcccacctg
     2701 caaaccacgt cacccccgac tgcgaaaagt caggtcgtgg cgcgtagcgt acctggtctg
     2761 ttcccaagaa tccgattgct ccacccaatg gtcgaaaaca tttcgtcagg caggcttcgc
     2821 agtggaatgc caacaagcca ctgcgtgctt gccacccacg agatgtcccg atgtcgcggt
     2881 gttggacctg gattcactgg gccgaacgtc ggacgcagtg catcaaaaaa ttcgaaacgc
     2941 ttgccctcaa acgcctatca tcgtcgtctg ctcaaaagac gatgcgctgg aagcacgccg
     3001 ctggaaacgc accgccacct ggttcgtccc caaagcagtc ggcgccgatg gtatccttcg
     3061 attgctcgaa gaactgcaac cgaaagtggg aacggcccgt cgcaaacgat cggaatagcg
     3121 gttccgaagc ctatttcgcc caaccctcaa aaatcatcgc cgaccgggaa tgaaatgggg
     3181 ggctcgtttg ctgtagccca cgtacatcgg ccacgaacct caaccccttg cgaaaggagc
     3241 cccaagccca tacattctcg cagcctcctc cctttggcaa cgcacggtcc tcggatcgtg
     3301 cgttgccttt ttgtgccttt gttagggcgc aaaaaccctc tcgagccctt tcccctcccc
     3361 ttttccacct ccagaagaaa cactcctcgc ccacaacgtc atcgtcgggc agcaacggct
     3421 cgcaacaaat gcttttgcgt catcgcaaac acgaaaagcg cgggcgcact gaggatgaca
     3481 ttgccagcca tcacgatatg ccatcgaacg cctgtccctt gctctcgcag caatgcgaca
     3541 gccaatggca tggttcgcac cgattcatcg gtggtcatga ccaaaggcca gaaataatca
     3601 ttccaatgaa agacgaaact tagcaaaaac aacgatgcca gggtgggcgc aagcatggga
     3661 gcaaggattc ggtagacgat cgtcaactcg gaggcaccat cgagccgggc ggcctcgatg
     3721 agttcatcag gaatggcaag aagggcctgc cgaaccatga aagtacccaa cgcgctggcg
     3781 ccaaaaggca agaccagagc ggtcatcgtg ttgacgagtt tcacttgggc gagcatcaga
     3841 aacaccggca cgaacgtgac ctgggccgga atgagcaagc aggccatgac ggtgagaaat
     3901 gccgcaccgc gtcccacgaa tctcagtttc gccaaggcat agcccgccgg caacaccaac
     3961 atgatttgca gcgccgcgat cgatagcgcc atgacagtcg tattgagcaa gtatcgcccg
     4021 accggaatgc ttcgaaacac ctccgcgtag gcttccccgt gaaaagccga aggcaacgga
     4081 gccaacggcg cgttcatgat gtctttcagg gacttgaacg aaccgagcac catccataga
     4141 taaggaccga gccatgccac ggcgacgatc aaaagcactg cattcagcag ataacgagcc
     4201 aggccctttt gcttcatcgc gaccgtctcc ccatgcgcca agcccaaagc tgtaccaggg
     4261 tcaaaaggag caaaacacca aaaaagacaa cgaccattgc gctggcgcgc cccgcccgaa
     4321 gattcaataa tacatgctcg tagatggcgt acacgaaaac cgtggtggaa tgggtagggc
     4381 ccccttgggt catcacccga acgatatcga acgcttgaaa cgagacgatc aggctcgtgg
     4441 ttcccacaaa agcggtggtg ggacgcagac acggccacgt aatatgaacg aatcgcgcaa
     4501 gaggacctgc accatcgagc gacgcggctt cgtaaaggtc ttccgggata tcttgacggc
     4561 ccgccagaaa gacgaccata gaaaatcctg ttattttcca aatagttaca ccagctaagg
     4621 cataaatggc ggtgcttgga ctcgtcagca gtcccgcttc tccaattccc aacgctttgc
     4681 aagcacgacc aacaaggccc gcgtcggcat cgagcaacca catccacaat agggccacgg
     4741 aaacccagga gacgacgtac gcgctaaaga cacaacttcg gacgaaagcg acgaattttc
     4801 cttttcgatt gacagccagg gcaagcagga ggccgaggaa catggaacca gagacgacga
     4861 cggcggcgta cgacagggtg atggtcaagg tgcgaaggat ttcgccgctg gagaatagtc
     4921 tttcgtagtg agcgaggccc acaaagcgcg gtggggtaag caggtcccag cgatacaggc
     4981 tacggtaggc ggcgaggccc aggggaaaaa ggaaaaaagt ccctaaaatt ccaagggttg
     5041 gcgccaaaaa gagataggga tggaggcggt gtcgagggcg tagcatggtg cctcaggtat
     5101 cctccagagc ttgttttcgg gcttcgttta gcaggctgcg ggcgtcttgg cggttcaaaa
     5161 ccgcttcttc gagacgcggg tcgacgcatt cgcgttgcac gcggaacagg ctttcggccc
     5221 aaggccaggg catggcgtat ttcagttggt ccatggcgac gcggtcgttg gggctttttt
     5281 ggtaaaatcc ctcccgttcg aggcgtgcga cggcatcgga ggcgacgggc atatatccgg
     5341 tgctggtggc ccattcgatg gtttgttcgg gctgcatcat ccagcgtagg aagtcccagg
     5401 cggcttgttt ttcgtgatcg ggggcgcttt tcatcatcac gaagaaggtg ccgccggtgg
     5461 gaacggcgcg tcggcgaaag cccggcaaag gagcggcgcg gacgggaaag gtggcgtgtt
     5521 cttcgagata acgcaaaaac gccgtggagg tgtagatcat ggcggcgcgt cccgcgagaa
     5581 agtcctggtt ggtgacttgc caggcgttgt aatcgcgtcc ggggggtgga cgcatggttt
     5641 tgtcttcgtg aaccatgcg
//
LOCUS       DAOSOR010000387         5658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14443_length_5658_cov_3.802427, whole genome shotgun sequence.
ACCESSION   DAOSOR010000387 DAOSOR010000000
VERSION     DAOSOR010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14443_length_5658_cov_3.802427"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PK710_17825"
     CDS             <1..522
                     /locus_tag="PK710_17825"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HOT11641.1"
                     /translation="RMAQRAGLSITRSAKRLLLVISLAGVLIVVGAPWLRAHGFEFMS
                     GILYDALRPVCHQIPSRSFHYHEYPWALCSRCSALLIGGALGLLLVPIIRSLDTAVPK
                     RHWLLIAAIPSAVDVAVGWLGIPNTFLSRTVTGGLLGSVTAFYLVPAMVAAMQELLVH
                     RSCRQEPVAHLGQ"
     gene            530..1279
                     /locus_tag="PK710_17830"
     CDS             530..1279
                     /locus_tag="PK710_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11642.1"
                     /translation="MKPSKVKPAVLGGVVAGLLSAILGIIPFVNYCCCLWSLLGGVLA
                     VHLYNKSLNKEGVPMADGAICGAIAGGIAAAIYVVIGIPLNIFVGGIIVGLMSGLADT
                     SLPMGHVAGNIVSSILAGIVFAIAIAVLTTIGGVIGALIFAPKKGAGAPPMGGVGGPG
                     AGTPAGGSPYGAPPAGPVGGMPPAAPPGSPYGAAPGGGASPGSPYGPPGGMPPAAPPG
                     SPYGAPGGPVPPGPGGGFNPPGGYNQGGFGR"
     gene            complement(1472..3091)
                     /locus_tag="PK710_17835"
     CDS             complement(1472..3091)
                     /locus_tag="PK710_17835"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOT11643.1"
                     /translation="MPAFIYQDPFPLSHQDATSYRLVTHEHVSVTDFQGTPMLVVAPE
                     ALRRIANEAMRDVSFLLRPKHLEQVAAILDDPEASPNDRGVAVAMLRNAEVSAKMQLP
                     FCQDTGTATVVAKKGQQVFTGVKDEAYLSEGIFDTYVNENLRYSQNAPLTLYDEVNTK
                     TNLPAQIDIYATEGMEYNFLFIAKGGGSANKTYLFQETKATLNPGTLEKFLVSKMMTL
                     GTAACPPYHLAFAIGGTSAEANLKTVKLASAGYLDGLPTQGNEYGQAFRDVEFEKTLL
                     EAAYRSGFGAQFGGKYFALDVRVIRLPRHGASCPIGMGVSCSAHRNILAKIDEKGIWV
                     EQLEKNPGRFIPDKYRGKHDHGAIKIDLQRPMKEILAELSKHPVRTQLSLTGTIIVAR
                     DIAHAKLKERIDRGEGLPQYVKDHPIYYAGPAKTPAGLPSGSFGPTTAGRMDSYVDLF
                     QSQGGSMVMIAKGNRSKAVTEACKKYGGFYLGSIGGPAALLAQENIKKVELLEYPELG
                     MEAIYRIEVVDFPAFILVDDKGNDFFEQLSS"
     gene            complement(3201..3740)
                     /gene="pyrR"
                     /locus_tag="PK710_17840"
     CDS             complement(3201..3740)
                     /gene="pyrR"
                     /locus_tag="PK710_17840"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOT11644.1"
                     /translation="MLGPEPMQEILTKEAASQGLQRLAAEIVDRLSGAEELALVGIRR
                     GGAPIAVHLASLIEKNTGIQVPIGAVDITLYRDDAPAPLPNPRIGKSDVPFDVRSKKI
                     VLVDDVLYTGRTVRAAMHALLDYGRSRAILLAVLVDRGGRELPIQPDFVVMSTEVSAT
                     EQVEVQASNDGFRAIVVGG"
     gene            complement(3790..>5658)
                     /locus_tag="PK710_17845"
     CDS             complement(3790..>5658)
                     /locus_tag="PK710_17845"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOT11645.1"
                     /translation="QKTPNPQNKTTASPTRPPSGFRASRVLGTFAAVLFVLAGSTWAW
                     SCSKDKPSSPSVSTTPSHSQPSVFIQENKPSPVAVASAPADASAVDDTPDEVPEAPWE
                     GPWVGALVPTAPVYVSPFRTRENMIGYLRHGTKAPVTSTKPLQKDNCKEGWYRLHPYG
                     FVCGSHVTTNLNHARFQAGIVPPDLEALVPYKYAYNNRNGTPLYRRIPTAEEMDTYEP
                     DRRAEIKAKEAKAKEAKAKRTAAARGEHANASPPSVSSVASAVASSHESVPSPSHSSL
                     AVPRSAAEATTGDDLPLLPLPGEVVADAGLDDAQAQEPEEKPWWMRDEIDKSDLTLDH
                     MHEGSDAVLAKRMARGFFVAVDKTFRKNGRFWYHTTEGLLAPADRMSINGPPTFQGVE
                     LIGQDAPSLPVGWTRVSAAKYTLGNDGKTFRRKGTVERFTMVSLTGKQMTEGSTQYVE
                     AKAGFWLRGSQVASTAPGPAPSGLLPKERWIDINLSTQTLVAFEGDTPVFATMVSTGK
                     KGQTKKTDHSTVKGTFRVREKHVAATMDGDGAAPGEGPYSIQDVPYVLYFERSYAVHA
                     AFWHNNFGTRMSHGCVNMAPADAKRIFFWSAPLVPPGWHGAWSTKEHPGSFVVVHD"
BASE COUNT         1087 a         1634 c         1620 g         1317 t
ORIGIN      
        1 aggatggcgc agagggcagg attgtccata acgcggtcgg cgaagcggtt actgcttgtc
       61 atcagcctgg ccggtgtctt gatcgtcgtg ggtgctccct ggcttcgcgc tcatggtttc
      121 gaattcatga gtggcatcct gtacgacgcg ctgcgccccg tttgccacca gattccttcc
      181 cgtagtttcc actaccacga gtacccatgg gccctttgct cgcgttgctc cgcgctgctc
      241 atcgggggcg cactcgggct gctgctggtt cccatcattc gttcccttga taccgccgtt
      301 ccgaaacggc attggttgct tatcgctgcc atcccttctg ccgtggatgt ggctgtggga
      361 tggctgggta tccccaacac gtttttatca aggaccgtca ccggcggcct tcttggttcc
      421 gttaccgcat tttatcttgt tccggccatg gtggccgcga tgcaagagct gctcgttcat
      481 cggtcttgtc gccaagagcc agtagcccac ttgggccaat gaggagtcca tgaaaccaag
      541 caaagtcaaa ccagcggtcc ttggaggtgt ggtggccggt cttctgtcgg ccatcctcgg
      601 catcattccc ttcgtcaatt attgttgttg tctgtggagc ttgcttggcg gcgttttggc
      661 cgtgcatttg tataataaat cgctgaataa agaaggcgtt cccatggcag atggggcgat
      721 ctgtggcgcg attgccggag gaattgcggc ggccatctac gtcgtgattg gcatcccttt
      781 gaatatcttt gtgggtggga tcatcgttgg ccttatgtca ggcctcgcgg ataccagcct
      841 tccgatggga catgtggctg gaaacatagt ctcatcaata ctcgccggca ttgttttcgc
      901 cattgcgatt gccgtactta cgacgatcgg aggcgtgatc ggcgccttga ttttcgcccc
      961 caaaaagggt gcgggagccc ctccgatggg aggggtgggt ggtccaggcg cgggaactcc
     1021 cgctgggggt agtccttatg gtgcccctcc cgccggtccc gtgggtggta tgcctcccgc
     1081 tgcccctcct ggcagcccct acggcgctgc tcccggcggc ggtgcctctc ccggctcacc
     1141 ctatggccca cccggaggta tgcctcctgc tgcccctcct ggcagcccct acggcgcacc
     1201 tggtggtcca gttccccccg gccccggcgg cggcttcaat ccgccaggtg gctacaacca
     1261 gggcggattc ggtcgataac cgctcgtccc ctccaatcct ttccttcctg ttccttggct
     1321 tgattatcct ctgatggaag gcctgcccga ctagccgggc tgcctcaccc cagccgatag
     1381 gcttcgtgtg aataaggcgg cgattgtagg tgtaggtaat cgctgtcgtg gcgatgagcc
     1441 gtctcgcgcg gtgttaggga aaataaaggc tttacgagga caattgctcg aagaaatcgt
     1501 tgcccttgtc atccaccaaa atgaacgcgg gaaagtccac gacctcgatt cggtaaatgg
     1561 cttccatgcc aagctcggga tactcgagca attccacctt cttgatattc tcttgtgcga
     1621 gaagtgccgc gggcccccct atcgaaccta gataaaagcc accgtatttt ttgcatgctt
     1681 ccgtcaccgc tttgctgcga ttgcccttgg cgatcatgac catcgaccca ccctgggatt
     1741 ggaaaagatc cacatacgaa tccattcttc ccgcggtggt tggaccgaac gagccggaag
     1801 gcaagcccgc cggcgttttc gctggcccag cgtaataaat cggatgatcc ttgacgtatt
     1861 gcggaagtcc ctcgcctcgg tcgattcgct ccttgagctt ggcatgcgcg atatccctgg
     1921 cgacgatgat cgtgcccgtc aacgatagct gggtacgaac ggggtgcttg ctcaattcgg
     1981 caaggatttc tttcatggga cgctgcaagt cgatcttgat agcgccgtgg tcgtgttttc
     2041 ctcggtactt atccgggatg aaacgccctg gatttttctc gagctgttcg acccaaatgc
     2101 ccttttcatc gatttttgcc aggatatttc gatgggcgga gcacgacacg cccatgccaa
     2161 tcggacagga agccccatgg cggggaaggc gaatcacacg cacgtcgaga gcgaaatact
     2221 tgcctccgaa ttgggcgcca aaaccggaac gatacgcggc ttcgagcagt gttttttcga
     2281 attccacatc gcgaaatgcc tgaccgtatt cattgccttg ggtaggaagc ccgtcgagat
     2341 atccggccga tgcaagtttg acggttttga gattggcttc cgcggacgtg cccccgatgg
     2401 caaacgccag atgataaggc gggcaagcag cggtcccgag ggtcatcatc ttgctcacca
     2461 aaaacttctc gagcgtcccg ggattgaggg tggctttggt ttcctgaaac aaataggtct
     2521 tgttcgcgga accgccgcct ttcgcgatga acaagaaatt gtattccatc ccctccgtcg
     2581 catagatgtc gatctgcgcg ggaagattgg tcttcgtatt gacctcgtcg tagagcgtga
     2641 gcggcgcgtt ttgtgagtac ctcaggtttt cgttgacata ggtgtcgaag atgccttcgc
     2701 tcaaatacgc ttcgtctttg acgccggtga aaacttgctg accttttttc gcaacgaccg
     2761 tagcggtgcc cgtgtcttga cagaagggta gctgcatttt ggcggatacc tcggcgttgc
     2821 ggagcatggc tacggcaact ccccgatcgt tgggcgaggc ttcggggtcg tccaagatcg
     2881 cggccacctg ttcaaggtgt ttcggacgca gcagaaagga cacgtcacgc atcgcctcgt
     2941 tggcaatgcg tcgaagggct tccggagcca cgacgagcat gggagttcct tggaagtcgg
     3001 tcaccgatac gtgttcgtgg gtgacgagac ggtaggaggt ggcatcctgg tgggaaaggg
     3061 ggaaggggtc ctggtagatg aacgcaggca tgacgtgctc cttggtgtcg ggttcgtaga
     3121 ttcactcgaa ggtgaacgcg ccgataaggt acacgtttga gtcccacacc ggaagaggcg
     3181 ggtgtgggaa cactcggttc tcatccaccc acgacgatgg cccgaaaacc atcgttcgac
     3241 gcttggacct cgacctgttc ggtcgctgag acttcggtgc tcatcacgac gaaatcaggc
     3301 tggatcggca gttctcggcc ccctcgatcc accagcaccg ccagcaaaat ggcgcgggag
     3361 cgaccataat ccagcagcgc atgcatcgcc gcgcgaaccg tgcggccggt atacaataca
     3421 tcgtcgacca acacgatctt cttgctccgt acatcgaaag gcacgtcgga cttaccgatg
     3481 cgcggattgg gaagtggcgc gggagcgtcg tcacggtaca aggtgatgtc cactgcgcct
     3541 ataggtactt gaattcccgt gtttttttcg atgagcgagg cgagatggac agcgatcggt
     3601 gccccccctc gtcgaatgcc cacgagagcg agttcttcag caccgctcaa ccgatccacg
     3661 atttcggcgg caaggcgctg tagaccctgg gacgctgctt ctttcgtcag gatctcttgc
     3721 ataggctccg gccctagcac ggcagctcat caaaacaaac cgctggaaga caaatggggc
     3781 tcctttcttt caatcgtgga cgaccacgaa tgagcctgga tgctcttttg tcgaccaagc
     3841 gccatgccag cccggtggaa ccaaaggggc ggaccaaaag aaaatccgtt tggcatctgc
     3901 gggagccatg ttcacgcaac catgactcat gcgcgtcccg aagttgttat gccaaaatgc
     3961 ggcgtgaacc gcataggaac gctcgaagta caacacgtac ggaacatcct ggatgctata
     4021 aggcccctcg cccggcgccg ccccatcgcc gtccatcgtc gccgcgacat gcttttctcg
     4081 aacgcgaaag gttcctttga ccgtactgtg atccgtcttt ttcgtctgac ccttctttcc
     4141 cgtcgatacc atcgttgcaa ataccggcgt gtctccctcg aaagccacaa gcgtttgtgt
     4201 gctcagattg atgtcaatcc atctttcctt gggcaataaa cccgagggcg caggaccggg
     4261 cgccgtcgag gcaacctgcg aaccccggag ccaaaatccc gctttcgctt ccacgtattg
     4321 cgtgctcccc tccgtcatct gcttgccggt caacgaaacc atcgtgaagc gctccactgt
     4381 ccctttccga cgaaatgtct tgccgtcgtt tcccaacgtg tacttggccg cgctcacacg
     4441 cgtccagccc acaggcaacg agggggcgtc ttgtccaatc aactccactc cttgaaacgt
     4501 gggcgggccg ttgatggaca tccgatccgc aggcgcaagc aatccctccg tcgtgtgata
     4561 ccaaaatcgc ccgttttttc gaaaggtttt gtccacagcg acaaaaaatc ccctcgccat
     4621 tcgcttcgcc agcacagcgt cagaaccctc gtgcatgtga tcgagcgtca aatccgactt
     4681 gtcgatttca tcacgcatcc accatggctt ctcttctggt tcctgcgctt gcgcgtcgtc
     4741 caatcccgca tcggccacaa cctcgccagg caaaggcaac agaggcagat cgtcgcccgt
     4801 cgttgcttct gccgcggacc gtggaaccgc aaggctcgaa tgcgatggcg acggaacgga
     4861 ttcgtgcgat gacgctaccg cggaagccac cgacgatacc gatggcggtg atgcgttggc
     4921 atgctcgcct cgcgccgcgg cggttcgctt ggctttggct tctttggctt tcgcttcctt
     4981 ggctttgatt tcggcacgac gatccggttc gtacgtatcc atctcctcgg ccgttggtat
     5041 cctccgatac aagggtgtcc cgttacggtt gttgtaagcg tatttgtacg gaacgagagc
     5101 ttccaagtcc ggtggaacaa ttcccgcctg aaatcttgcg tgattgaggt tcgtcgtgac
     5161 gtggctgccg catacgaagc catagggatg aagacggtac cagccttcct tgcaattatc
     5221 tttctgaagg ggtttggtgg acgttacggg tgctttggtg ccatgacgca agtagccaat
     5281 catattttcc cgcgttcgga atggggagac atacacggga gccgtgggga ccaaggcgcc
     5341 aacccacggc ccttcccacg gagcctctgg aacttcgtcc ggggtgtcgt ccacagcact
     5401 ggcgtccgcg ggagcagaag ccaccgcaac cggcgaaggc ttattttctt gaataaaaac
     5461 agatggttgt gaatgagagg gggttgtgga gacagaagga gaggatggtt tgtctttcga
     5521 acaggaccag gcccaggtcg agcctgcaag gacgaaaagc actgctgcaa aggttcctag
     5581 cacgcggctt gctcgaaagc cggaaggggg tcgcgtgggt gatgccgtgg tcttattctg
     5641 aggattgggc gttttttg
//
LOCUS       DAOSOR010000388         5654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14455_length_5654_cov_4.059118, whole genome shotgun sequence.
ACCESSION   DAOSOR010000388 DAOSOR010000000
VERSION     DAOSOR010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5654
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14455_length_5654_cov_4.059118"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PK710_17850"
     CDS             <1..307
                     /locus_tag="PK710_17850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOT11646.1"
                     /translation="VVFRSFYLLHKPVGRLSGLDHLDAIMRNGQAQVLFSSHDLAGAL
                     AQDRHGMPSFRVTPDGDLQREMAIRLAVNVAMFVLCSNYKDDQVHAPILMRRRARDR"
     gene            307..2493
                     /locus_tag="PK710_17855"
     CDS             307..2493
                     /locus_tag="PK710_17855"
                     /inference="COORDINATES: protein motif:HMM:NF018757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HOT11647.1"
                     /translation="MSIALSTDLPLWAGILAGLLLVGGLAMVSWELALHRRRAIIVAS
                     TALAATLLLTLAVLRPLRVQAKYTTVGPRVLVLVDRSLSMALSGDGDQRSRVANHVVG
                     ELRRTHSAARWSIHGFGSGAAFSWDESNDTAPDQPSSDLLSAFAALSESSEEKPSAVV
                     IVSDGRLDRPPEGVSEQQIRSALSSWNMPIHTVAVASTDMSDASIRDVRTAGVSVAHQ
                     PFRLTVTVACTGRLVCENIPLTVLELVEEGAPVVLASGTVRLERGIGTVELPITLDRA
                     GSRVVRVSIRAPEGDELPANDQRILTFNVTRDRVRILHIAGRPTYDTRAMRQWLKADG
                     SLDVVAFFILRSLSDDVNATPDELALIRFPVDELFSEHLSSFDAVVLQDFDALSYGLT
                     PYLSNLSDYVNKGGGLVMVGGLHGFSAGNYAGTPLETVLPVRLPKEEHGIDTAPFVPR
                     MTVAGRETPVLEAVRALYGDRLPQMSGTNLLDEARDGAVVLWEHPTLTTTRGKPMPVL
                     ALGEHGNGRVVALSVDGTHRLAFSEMASQMAGRGYGALWDALLGWLMRDPRYEPARVE
                     LLSPCLAGRATPVRIHRVPGLQGEVQARLVALGSQKALPDLVGSKSTDRSFTAELPAL
                     DAGGYALRLQIGKGASTRFDFACEQGGDEWADPRPDVTRLQTIAKASAGSFVGAKDVS
                     KLTFPQSTPVATERQESPLLPAWVWSLAAAMALGIHWIARRWSGLS"
     gene            2748..4415
                     /locus_tag="PK710_17860"
     CDS             2748..4415
                     /locus_tag="PK710_17860"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOT11648.1"
                     /translation="MTGSLTVLLAAAAGFALGCKNTNQGSSAASSSTASDTAPVPSAQ
                     PDGVDLTETAQDVPILGARGLYVMVRDRPDMKTSNKLGFIRLGGIVERSSNPVAGTGC
                     KGGWYEIKPQGYACLDADGTIDRNDPILRAASRRPALDKPMPYAYGFVRAVLPYYLRI
                     PTEKEQFESEFQLKEHLEWWEREGKKINATLPLGANDVFIDSLGVPDDSKRVLKLSTE
                     MSDGERFGGTTNDDPIPWWLEGGRKIPNVAEFKVPEYAVFADRARRFSGLTFVGSFPT
                     GADSLRRRFAITEDLRLVPTTKIKPDGGPTFHGVEVNAERPLPFAWIKNRDAKRYKIE
                     GTTVRAFKQRATYREVIPLTGKKQFVDKRLYYETEGGKWVRSRDIAIASTPTEMPAGA
                     KSGEKWIDISIRQQVLILWEGTTPVYATLISTGQDMLGDPKTTKSTVLGTFRIESKHA
                     TTHMDSNEGLTRGTGDPEYGKTKRRGQGTFLLQNVPWVQYFKGSYAIHATYWHDVFGT
                     ARSHGCVNLTPIDAHRVFFWTHPSLPKGWHGVYPSKPQEGTLVYIHE"
     gene            complement(4441..5502)
                     /locus_tag="PK710_17865"
     CDS             complement(4441..5502)
                     /locus_tag="PK710_17865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11649.1"
                     /translation="MRRVDRMDMHLFCFFCLLLSCFPISCKKKESLSGYIPSASTPPV
                     TSLATNSPHPVGNDDGILHGLWSQRPFLMRAALVSIHPKWMTITILDREARCDSAKLV
                     ENDLGIQMTIPSGPNNDFFAGHDIPTEIRLHGAGGVSRIPAGHVVVRVESFDPQHDES
                     VRGYLSFRFRTASDADAPSYASEGSFSARVCNNRPTTASARHAPTQNSSVSGIVAGRP
                     VTLTSMLAYVRDDGYGSPILMIKGFEGDVVCHTTRSATPYLYGAEVAIEPDSKRFLHA
                     PLPADWMVQMRMYQFSERSVQTAHGAGWLQIESADLSDGGVVRGQLVADNPQDELEWS
                     FSLAGQFEAKMCGVERRAW"
BASE COUNT         1172 a         1437 c         1781 g         1264 t
ORIGIN      
        1 cgtcgtattt cgatctttct atctgctaca caaacctgtc ggtcgcctgt ctggtctcga
       61 ccatctcgat gcgatcatgc gcaatggtca agcccaagtg ctgttttctt cccacgactt
      121 ggcgggagcc ctcgcgcaag accgacacgg aatgccatcg tttcgtgtca cgcccgatgg
      181 tgaccttcaa cgggaaatgg cgattcgtct cgcggtcaac gtggccatgt tcgtattgtg
      241 ctccaactac aaagatgatc aggtgcatgc acccatcttg atgcgtcgaa gagcacgaga
      301 ccgttgatgt ccatcgcgtt gtctacggat ctccccctgt gggctggaat tcttgcgggt
      361 ttgctgctcg tggggggatt ggccatggtg agctgggaac tcgcccttca tcgtcgtcga
      421 gccattatcg tagcctccac ggccttggcc gcgacgttgc tactgacgct cgctgtgttg
      481 cgtccgttgc gggtccaagc gaagtacacg acggtcggcc ctagggtgct ggttttggta
      541 gatcgatctt tgagcatggc attatcggga gatggggacc aacgcagtcg cgtagccaac
      601 cacgttgtcg gtgaattgcg tcgcacccat tcggcggcgc ggtggtcgat tcatggattt
      661 ggttctggcg ccgcgttttc gtgggatgaa agcaatgata ccgctcccga tcaaccttcg
      721 agcgacctgc tctcggcttt tgcggcgcta tcggagagtt ctgaagaaaa gccatcggcg
      781 gtggtcatcg tctcggatgg gaggctcgac cggccaccgg aaggcgtatc ggaacaacaa
      841 atacggtccg cattgtcatc gtggaacatg ccgattcaca cggttgccgt ggcctcgacg
      901 gacatgagcg atgcgtcgat tcgagacgtt cgcacggcgg gggtttcggt ggcgcaccag
      961 ccatttcgat tgacggtcac ggtggcttgt acggggcggc ttgtttgcga gaacatccct
     1021 cttacggttc ttgaattggt ggaagaggga gcgccggtcg ttcttgcgtc gggcacggta
     1081 cgtttggagc gagggatcgg aacagtcgag ttgccaatta cgctggaccg tgcaggttcg
     1141 cgcgtggtgc gtgtatcgat tcgcgccccg gaaggtgatg aactcccagc caatgatcaa
     1201 cgcattttga cattcaacgt cacccgcgac cgggttcgaa ttctacatat cgccgggcgt
     1261 cccacctacg acacgcgagc gatgcggcaa tggctcaaag cggatggctc cctggatgtc
     1321 gttgcatttt tcattcttcg ttctctttcg gatgatgtga atgcgactcc ggatgaactg
     1381 gcgttgattc gatttcccgt cgacgaatta ttcagcgagc atctttcgag tttcgacgcg
     1441 gtggttcttc aggattttga tgctctatct tatggactta cgccctatct ttccaacttg
     1501 agcgactatg tgaacaaggg aggggggttg gtcatggtgg ggggattgca tggattttca
     1561 gccggcaatt atgcgggtac tcctctcgaa accgtgcttc ccgtgaggtt acccaaagag
     1621 gagcacggga tcgacacggc accattcgtt ccgcgaatga cggtggcggg tcgagaaaca
     1681 ccggtattgg aggctgttcg cgcgctatac ggcgaccgat tgccccagat gtccggcacc
     1741 aaccttctcg atgaagcgcg cgacggtgct gtcgttttgt gggaacatcc gacactgacg
     1801 acaacccgag gcaagccgat gcctgttctt gcgctagggg agcatggcaa tgggcgtgtg
     1861 gtggcgcttt cggtggacgg aacccatcgg ttggcattta gcgagatggc ttcgcagatg
     1921 gcggggcggg gatacggtgc gctttgggat gcattgctcg gatggttgat gcgcgatcct
     1981 cgttatgagc cagcccgggt cgagttgctt tcgccatgtc ttgcgggacg agcgacaccg
     2041 gtacgtattc atcgggttcc gggattgcaa ggggaggttc aggctcggct cgtagcgttg
     2101 gggagtcaga aagcgcttcc cgatctcgtg ggatcaaaat caacggaccg ttcatttacg
     2161 gcggagctgc ctgctttgga tgcgggggga tatgctttgc gcctgcagat tggcaaaggc
     2221 gcttcgacac gtttcgattt tgcttgtgag caaggcgggg acgaatgggc tgatcctcgt
     2281 ccggatgtga cacgtttgca aaccatcgcc aaagcatccg ctggctcttt cgtaggcgca
     2341 aaagacgtat ccaaactcac gtttccgcaa tcgacgcccg tggccacgga acggcaggag
     2401 tctcctctgc tgccggcctg ggtgtggagt ttggcggcag cgatggcctt gggaatccat
     2461 tggattgcgc gtcgctggtc cgggttgtca tgacgtcggc ttcatgggcc acccgcggac
     2521 ggctagctta tttttcggaa acgcggtcga gaggatgcat gggtgaggtg gcgtccatgg
     2581 catggaacgt gtggtgtgac agggcgtgtg ggagaaacga aacgcgcggt agatcaggca
     2641 tcagaatcgg ttgatgattg gttgtatgca tgaatgaaat ccgcgtggga gggcgtgcat
     2701 gctttacaaa ccggaggaag gtttggcata gcaacgggat gcgatcgatg actggcagcc
     2761 ttacggttct gctagccgcc gccgcaggat tcgccttggg atgcaaaaac acgaaccagg
     2821 gttccagcgc ggcttccagc agcaccgcgt ccgatactgc acctgttcca tccgctcagc
     2881 ctgacggtgt cgacctaacg gagacggcgc aagacgttcc gatacttggt gcgagagggt
     2941 tgtacgtcat ggtgcgcgac cggccggaca tgaagacgtc caacaagctt gggttcattc
     3001 ggcttggcgg gattgtggag cggagttcca acccggtagc gggcacagga tgcaagggtg
     3061 gttggtatga aatcaagcct caagggtatg catgccttga cgccgatggc acaatcgatc
     3121 ggaacgatcc gatcttacga gcggcgagtc gtcggcccgc cttggacaaa ccgatgccct
     3181 acgcgtatgg gttcgtgcgc gcggtgctgc cctattacct tcgtattccc acggaaaagg
     3241 agcaattcga atcggagttt cagctcaaag agcatctcga gtggtgggag cgggaaggaa
     3301 agaaaatcaa tgcgacgttg ccgttgggag ccaacgacgt attcatcgat tcgttggggg
     3361 tgcccgatga cagcaagcgg gtcttgaagc tttccacgga gatgagcgac ggggagcgat
     3421 ttggggggac gaccaacgac gatcccatac cgtggtggct cgaaggggga agaaagatac
     3481 cgaacgtggc ggaattcaaa gttccagaat acgcggtatt cgccgatcgg gcgcgacgtt
     3541 tttccgggtt gacgttcgtg ggttcgtttc ctacgggagc cgattcattg cgtcggcgtt
     3601 ttgccattac ggaggatctt cgtctggttc cgaccaccaa gatcaaaccg gatgggggtc
     3661 ctacgttcca cggtgtagag gtcaatgcgg agcgtcccct gccctttgcg tggatcaaaa
     3721 accgggatgc caaacggtat aagattgagg gaacgaccgt tcgcgcgttc aagcagcgcg
     3781 ccacgtaccg tgaggtcatc ccgctgacgg gaaagaaaca atttgtcgac aagcggttgt
     3841 attacgaaac cgagggaggg aagtgggttc ggtccaggga tatcgccatc gcctccacgc
     3901 ccacggaaat gcctgcgggt gcgaagtctg gcgagaagtg gatcgatatt tccattcgtc
     3961 agcaagtatt gattctttgg gaaggaacga ccccggtgta cgcgacgctg atatcgacgg
     4021 ggcaagacat gcttggggat cccaagacga cgaagtccac ggtacttggg acatttcgta
     4081 tcgagagcaa gcatgccacg acgcacatgg actcgaacga agggctgact cgtgggacgg
     4141 gcgatccgga gtacggcaag acgaagcgtc gtggacaagg gacgtttttg cttcagaacg
     4201 tgccgtgggt tcagtatttc aaggggagtt acgccataca tgcgacatat tggcatgacg
     4261 tattcggcac ggctcggtcg cacggatgtg tgaatctcac gcccatcgat gcgcaccggg
     4321 tgtttttctg gacgcatccg agccttccga aaggctggca tggggtatat ccttccaaac
     4381 ctcaggaggg cacgcttgtc tacattcatg aatgatcgcg gaggggttgg atagtatcga
     4441 tcaccaggcg cgtcgttcca caccacacat tttggcttcg aattgtccgg cgagggagaa
     4501 ggaccattcc aattcatctt gggggttatc ggccacgagt tgtccgcgca ccacacctcc
     4561 atcggacaag tccgccgatt cgatttgcag ccatcctgcg ccgtgggcag tttgcacact
     4621 tctttcggag aactggtaca tcctcatttg caccatccaa tccgcgggta agggggcatg
     4681 caaaaaacgt ttggaatctg gttcgatagc cacctcggca ccgtatagat agggggtggc
     4741 gcttcgcgtg gtgtggcaca ccacatctcc ttcgaaccct ttgatcatga gaatgggcga
     4801 tccatatcca tcatcgcgca catacgctag catcgaggtc aatgtgactg gacgtcccgc
     4861 gacgataccg gagacggaag agttttgagt gggggcgtgt cgagccgagg ccgttgtcgg
     4921 ccgattgttg caaacacggg cggagaaaga tccttcggaa gcgtaggaag gagcgtcggc
     4981 atcggaggcc gttcgaaagc gaaagcttag atagccgcga acggattcat cgtgctgagg
     5041 atcgaacgac tcgacacgca cgacgacgtg tccagcgggg atacggctga cgcctccagc
     5101 gccatgcaag cggatttcgg ttgggatgtc atggccggcg aaaaagtcgt tgttgggccc
     5161 cgaagggatg gtcatttgga tcccgaggtc gttttccacg agtttggcac tatcgcagcg
     5221 tgcttctctg tccaggatgg tgatggtcat ccacttcgga tgaatggaaa ccagggcggc
     5281 ccgcatgaga aagggacgtt gagaccacag tccgtgcaaa ataccgtcgt cattgccaac
     5341 ggggtgaggg gaattggtcg cgagcgaagt gaccgggggc gtagaggccg agggaatata
     5401 tcccgatagg gactcttttt tcttacaact gattgggaag catgaaagca gcaaacagaa
     5461 gaaacagaaa aggtgcatgt ccatccggtc cacacgtctc acgatcgaac ccgaatggct
     5521 ttgggcaaaa cacgagccac aatcgggagc attcctgggg tttcgccatc catgtccaac
     5581 agaactcgtt gttcttttcg gtggggaatg gcttccacga cagagcccct cgtatgggta
     5641 accttatgga catc
//
LOCUS       DAOSOR010000389         5632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14526_length_5632_cov_4.449704, whole genome shotgun sequence.
ACCESSION   DAOSOR010000389 DAOSOR010000000
VERSION     DAOSOR010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5632
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14526_length_5632_cov_4.449704"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..257)
                     /locus_tag="PK710_17870"
     CDS             complement(75..257)
                     /locus_tag="PK710_17870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11650.1"
                     /translation="MTRIVEHIANLAQLLDESLQEKLWEYAELLYVEQIAFESTEPDH
                     WTVESFHNTRAYPLRE"
     gene            complement(456..848)
                     /locus_tag="PK710_17875"
     CDS             complement(456..848)
                     /locus_tag="PK710_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11651.1"
                     /translation="MTTHTRSIPYPLLIVALLTSALGTACRSEPQQQEAQYPPQQQYP
                     PQQYPAQQYPAQPYPAAYSQPGAQPPPGGVPCGSDADPQCPFGRCIAGKCGGCNTVEH
                     CKPGAACMQTPVGMTCVPGGMPGVPPAQ"
     gene            974..2125
                     /locus_tag="PK710_17880"
     CDS             974..2125
                     /locus_tag="PK710_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11652.1"
                     /translation="MNLKHSISFWATVGVVGLRGVAANAQTGDCFGAPCGLVRGVTIG
                     PIESALHPDKGYGSAPYARTLRELVQWGATWVALTPFGRTMNLRPTGVDLSFEAPFEK
                     NRQDVIKAIHMAHEQGLRVFLVPHLWVESGQWRAEIDPGSDEGWALWMDGYERFLLTW
                     ASVAEQAQVELLSVGVELRSWVTTSRAPRFVDMIRRVRNVYHGPLTYSANWDDVQDTV
                     ILGELDVIGINAFYPLTDSENSSFQRLVKGGNSVAKSVKELSSSWNRPVLFTEIGYTT
                     RPDPALRPWEWPDDMQGVVVDEKAQALAYKALLAPLVDEPSFAGFFVWRVYADPDDMS
                     QEAEWGFSPRGKRAELVVRDAFSAYWAADGPYEMGSSLVRWAATQPGLY"
     gene            complement(2201..3169)
                     /locus_tag="PK710_17885"
     CDS             complement(2201..3169)
                     /locus_tag="PK710_17885"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT11653.1"
                     /translation="MTSRLGLDFFINLVAAGLARGTPILFATLGGIITERSGVMNLGI
                     EGMMLVGAMTAFSTARSTNNALLGVCTGMLAGATLALAYAIVVVHLRADQVVSGLALV
                     FFGSGLSSMLGASLVDQKEATARIPSLSLSVLEDLPILGPVLFKQNLMVWLGLALVAC
                     GSWILFKTRWGLVLRAVGEHPAQADSLGISVLTVRTVCVMIGGAMAGLAGASLSLAIS
                     PGWVDGMTAGQGWVAVGLVIFARWNPGRALIGAWLFGTLRRLPLDLQGVPSLPVFHNP
                     NIGYFLSMLPYLFVIGAMIVGSRQALRRRLGAPASLGLPWFRGDRG"
     gene            complement(3166..4251)
                     /locus_tag="PK710_17890"
     CDS             complement(3166..4251)
                     /locus_tag="PK710_17890"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT11654.1"
                     /translation="MLKWHLEPRLDTTTRQRWIHTIASVAAALLVGAILLAGAGVDPL
                     EAYVHIIRTGLLSGYAVSDTIVKATPILLCALGASVAFRMKLWNIGGEGQLLLGAWAA
                     AGVALHWLPPTTPSLFMLLTMMLAGALAGAVTCAIAGVLRAWLHVNEIITTLMLNYVA
                     SLWVSYFVFGPWSDRGFPLTPSFPKGATLPRLTDIAASWPALGGMTAHAGAILALVAS
                     GVVSHALARTRWGFEIRVLGQNPTVGRLSGMNHARLTLTTMAVSGALCGLAGMTEVSG
                     VVHRLQDRFSPGFGFMGIIVAWLGKLEPWRMVVVAFLFGVLLVGGKEIQPGGVPQMLQ
                     GLILFVIVGSQFWLRFKIRVARTEPTS"
     gene            4250..4459
                     /locus_tag="PK710_17895"
     CDS             4250..4459
                     /locus_tag="PK710_17895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11655.1"
                     /translation="MTMFRRGLWVGWNIHPTSEPFTSTGQTHEPAMIKRIEPRGLSLL
                     RDGNDTTQLSGWERQDSHVMKTRSG"
     gene            complement(4521..>5632)
                     /locus_tag="PK710_17900"
     CDS             complement(4521..>5632)
                     /locus_tag="PK710_17900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT11656.1"
                     /translation="VWQLSIGQRQRVELLRALMRGARILLLDEPTAVLSPHEIHQFLA
                     SLRRLTAQGMSIALVSHKLEEIVQIADRFTVLRKGRGVVSGVPTASTSQTELASWMVG
                     QKIDKPCHDVRSPASDVEVLDVHDVHACGDKGFEALCHVSFKVFAGEILGVAGVSGNG
                     QRELADVLSGMRPITSGRITLCGNDVTHATPARFLRAGLAYIPENRRTQGAVTSLSLA
                     ENLALKTYRQPPIAKRWGLSRKEMRSFARRLVDTFHVQAVSLQQPAGHLSGGNLQRLI
                     LARELSHPISLLLAAQPTRGLDIAATRNIHDLFMAQRKAGVATLLISEDLDELSSLCD
                     RLIVLFRGRIVARFDRASFHRDAIGRAMAGIETSA"
BASE COUNT         1228 a         1503 c         1767 g         1134 t
ORIGIN      
        1 tcgatagccc gatcttggcg attcgattgc ccggatagta ggtgagatcg gtgtgttgat
       61 ggggtggatg ggagtcattc ccgcaggggg taggcgcggg tgttgtggaa ggattctacg
      121 gtccagtgat ctggttcggt ggactcgaaa gcgatttgct cgacatagag taactctgcg
      181 tattcccaca gtttttcttg aagtgattca tcgaggagtt gggcgagatt ggcgatatgt
      241 tcaacgatgc gggtcatggt gttttcacct cctgcgctgg gaagagcaag agggatgcca
      301 gggataaagt ggggaaaatg ccgaaggaaa cgaggcgtag gaagtggcgg tgcgccaccc
      361 caaagggggc cgaaaggcgc aaaaggctaa gaaaatcccg ggaaattctt caagtggcga
      421 agcgccaccc caaattggcg gtcgccagcg ggaggctatt gggcgggggg cacaccgggc
      481 attcccccgg ggacacaggt catgccgacg ggggtttgca tgcatgccgc gcctggtttg
      541 caatgctcga cggtattgca tccaccgcat ttgcccgcga tgcatcgtcc aaaggggcat
      601 tgggggtctg catcggagcc gcaggggaca ccgccggggg gcggttgggc accgggttgt
      661 gaataagctg cggggtaggg ttgggctgga tattgttgtg ccgggtattg ttggggtggg
      721 tattgctgtt ggggcgggta ttgagcttct tgttgctggg gttccgatct gcatgcggtt
      781 cccagtgcgc tggtaagcaa ggcaacgatg agcaacgggt aggggatgga tcgcgtgtga
      841 gtggtcatgt ttgactccgc acgggtgagc gagattcgaa ggaagtgtaa cctgtcggag
      901 acgcccggtg aatgatggta cgttggagcg gcccggaagt ctcccgcgac ttgcggggag
      961 gatacctaac tgcatgaatc tcaagcattc tatttccttt tgggcgactg tgggcgtggt
     1021 gggtctccgt ggcgtggcgg cgaacgcgca aacgggggat tgctttggag ccccttgtgg
     1081 attggtgaga ggcgtgacga ttggtcccat cgaaagcgcg ttacatccag acaagggcta
     1141 tgggtctgcg ccttacgcgc gaacgttgcg ggaactcgtc caatggggcg ccacgtgggt
     1201 tgcgcttacc ccttttggtc ggacgatgaa tcttcgtccg acgggcgtgg atttatcatt
     1261 cgaagctccg ttcgagaaaa accgccaaga cgtgatcaaa gcgattcaca tggcgcatga
     1321 gcaggggctt cgggtgtttc tggttcctca cttatgggtg gaaagtggtc agtggcgagc
     1381 cgagatcgat cctggttcgg acgaaggctg ggcgctgtgg atggatggat acgaacgctt
     1441 tttgcttacc tgggcgagcg tggccgagca agcgcaggtc gaattgctaa gtgtgggggt
     1501 tgagcttcga agctgggtga cgacctcacg cgccccacga ttcgtggata tgattcgtcg
     1561 tgttcgaaat gtctatcacg gtcctcttac gtactcggcc aattgggacg acgtacaaga
     1621 cacggtcatt ttgggtgaac tcgacgtgat aggcatcaat gcattctatc cacttacgga
     1681 tagtgaaaat tcctccttcc agcgtttggt aaaaggagga aattctgtgg caaaaagcgt
     1741 caaagagttg tcgtcatcgt ggaatcgtcc ggtgctattt accgaaatcg gatacacgac
     1801 tcgtcctgat ccggcgcttc gtccatggga atggccggat gatatgcagg gagttgtcgt
     1861 cgacgagaag gcccaggcgc tcgcgtataa ggcgcttctg gcaccgcttg tcgatgagcc
     1921 atcgttcgct ggttttttcg tttggcgcgt gtatgcggac ccggacgata tgtctcaaga
     1981 agcggagtgg gggttttcac cgcgaggcaa acgggcggag ctggtggtgc gagatgcttt
     2041 ttccgcgtat tgggccgcgg atgggccgta tgaaatgggc agcagcctgg tgcggtgggc
     2101 agcgacccag cctggacttt attgagtgcg tttgggatgg cttgaagcgc tattcttgaa
     2161 gccaattccc catgcgaagc gcgtcggcct cgccgtgggt tcatcctcga tcgccgcgaa
     2221 accaaggcaa tcctaacgat gcgggcgctc caaggcgccg tcgcagcgct tgcctcgatc
     2281 ccacgatcat cgcgccaatc acgaaaagat aggggagcat gctcaaaaaa taaccgatat
     2341 tgggattgtg aaagaccggc agactgggaa ctccttgcaa atccagcggg agccgtcgaa
     2401 gcgtgccgaa tagccaggca ccgatcaacg cacggcccgg gttccatctt gcgaagatca
     2461 ccaatcccac cgcaacccat ccttgcccag cggtcatccc atcgacccat ccgggagaga
     2521 tcgccagact gaggctcgcg cccgccaaac ccgccatggc gcccccgatc atcacgcaga
     2581 cggtgcgtac cgtaagcacg gatattccca gtgaatctgc ttgggcgggg tgttctccta
     2641 ccgctcggag gaccagcccc catcgcgttt tgaataggat ccaggagcca caagccacga
     2701 gagcgagccc caaccacacc atcagatttt gcttgaaaag cacgggaccc aggatgggaa
     2761 ggtcttccag caccgacaag gacaaggagg ggatacgcgc ggtggcctct ttctgatcga
     2821 cgagcgaagc gccgagcatg gaactcaatc cggaaccgaa aaatacgaga gccaaaccac
     2881 tgacgacttg atcggctcga aggtgtacca cgacgatggc ataggcgagg gcaagcgtgg
     2941 cccccgcgag cattccggta cacacaccta gcaacgcgtt gttggtagag cgtgccgtgg
     3001 agaacgcggt catggctccg acgagcatca tcccttcgat tccgaggttc atcacgccgg
     3061 agcgctcggt gatgatgccg cccaaggtgg cgaacaggat gggggttccc cgtgccaacc
     3121 cagcggccac cagattgatg aaaaaatcga gtccaagacg tgaggtcatg acgttggctc
     3181 cgtgcgtgcg acacgaatct tgaaacgcag ccaaaactgg gacccaacga tcacgaatag
     3241 aatgagtcct tgcagcatct ggggcacgcc ccccggctga atctctttgc caccgacgag
     3301 caagacaccg aaaagaaaag cgaccacgac catgcgccag ggttcgagtt tgcccaacca
     3361 ggcgacgatg atgcccatga agccgaagcc tggagagaag cgatcttgga gcctatgcac
     3421 gacaccggat acctcggtca ttcccgccag tccgcacagc gctccgctta ccgccatggt
     3481 cgtgagggta agtcgggcat gattcattcc ggaaagacga cccaccgtgg ggttttgtcc
     3541 taatacccgg atttcaaagc cccatcgtgt tcgtgcgagg gcatggctca cgacaccgct
     3601 tgccacaagg gcgaggatgg cgccggcatg ggctgtcatg ccaccgagcg ccggccaaga
     3661 cgcggcgatg tccgtcaagc gtggtagcgt ggcgcctttt ggaaacgaag gggttagagg
     3721 gaagccacga tcactccacg gcccaaatac gaaataggat acccacaggg aagcgacata
     3781 attgagcatc aatgtcgtaa tgatttcgtt gacatgcagc catgctcgca acacgcccgc
     3841 gatggcacag gtaaccgcgc ctgccaacgc tccggcgagc atcatggtga ggagcatgaa
     3901 cagcgacggc gttgtgggtg gcagccaatg aagcgccaca ccggccgcgg cccatgcgcc
     3961 gagcagcaat tgtccttccc ccccgatatt ccaaagcttc atgcgaaaag ccacggaagc
     4021 tcccagggcg cataacaaga tgggcgtggc tttcacgatg gtgtcggaaa ccgcataacc
     4081 actgagcagg ccagtacgaa tgatgtgtac gtaggcctcc agaggatcga ctcccgctcc
     4141 cgcaagcaat atcgctccca cgagcaaagc ggcggctacg gaagcgatgg tgtgaatcca
     4201 ccgctgccgc gtcgttgtgt cgagccttgg ttctaggtgc catttcaaca tgaccatgtt
     4261 tcgtcgtggc ctttgggttg gatggaatat ccaccccacg agcgagcctt ttaccagcac
     4321 aggacagact catgaaccgg cgatgataaa aaggatcgaa ccacgcggat tgtcgttgtt
     4381 gcgggatggt aacgacacga cgcagctctc gggatgggag cgacaagact cccatgtcat
     4441 gaaaacccgt tccggataga ggcaagctcg ctttggggcg ccgacgggtt cggtcgtgat
     4501 gacggttcgt gaatcgctcg tcatgccgac gtttcgattc ccgccatggc ccggccaatc
     4561 gcatcacgat ggaacgaggc gcgatcgaaa cgggctacga ttcgtccgcg aaacaacacg
     4621 atcaaccgat cgcatagcga actgagttca tcgagatctt cggagatcaa caaagtcgcg
     4681 acaccggctt ttcgttgggc catgaataga tcgtggatat ttcgcgtggc cgcgatgtcg
     4741 agcccacggg taggttgtgc tgccaacagc aaggaaatgg gatgggacag ttctcgcgcc
     4801 aggatcaatc gttgcaagtt gccgccggac agatggcccg cgggttgttg caacgagacg
     4861 gcctggacgt gaaaggtatc gacgagccgt ctggcgaacg agcgcatttc ttttcgggat
     4921 aggccccaac gtttggcgat gggaggttga cggtaggttt ttagcgccag gttttcggct
     4981 agcgaaagac tggtaacggc cccttgcgtg cgccgatttt cgggaatgta ggccagccct
     5041 gcgcgcagaa agcgtgctgg tgtggcatgc gtcacatcgt tcccgcaaag ggtgatacgt
     5101 cctgaggtga tgggccgcat tccactgagc acatccgcca gttctcgctg accatttccg
     5161 gatactccgg caacccccag aatttcgcct gcaaacacct tgaacgaaac gtgacacaag
     5221 gcttcgaatc ccttgtcgcc acaagcatgc acgtcatgca catcgagcac ctcgacgtcc
     5281 gaagccgggg agcgaacgtc atgacaaggt ttgtctatct tttgtccaac catccacgag
     5341 gccagctcgg tttgcgacgt gctcgcggta ggcacccccg atacgacccc acgacctttt
     5401 cgcaggaccg tgaatcggtc cgcgatctgg acgatttcct cgagtttgtg actgacgagg
     5461 gcaatgctca ttccttgcgc tgtcaggcgc cggagagaag caagaaactg gtgaatttcg
     5521 tgggggctga gcacggcggt gggttcatcg agcaacagga tacgggcgcc tcgcatcaag
     5581 gcccgcagca gttcgacccg ttgtcgttgc ccgatggata gctgccagac gt
//
LOCUS       DAOSOR010000390         5628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14537_length_5628_cov_4.915306, whole genome shotgun sequence.
ACCESSION   DAOSOR010000390 DAOSOR010000000
VERSION     DAOSOR010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5628
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14537_length_5628_cov_4.915306"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PK710_17905"
     CDS             complement(<1..213)
                     /locus_tag="PK710_17905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11657.1"
                     /translation="MGINPIGKSGRPGEFISPTTAAVDAPSPGQSVDSPEEFSLSKVE
                     STEATLKTSPLEQLKSGEISVEEYLDI"
     gene            complement(298..768)
                     /locus_tag="PK710_17910"
     CDS             complement(298..768)
                     /locus_tag="PK710_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11658.1"
                     /translation="MGVRPASSPSEVMMTPSAHRVTPPDGPTFGEVMRGSAQQFLRGA
                     SAAIHVLPSGPVVAASVRPGPLGMSIGPGISPAGVGGLGVTGPGSAPISGAQDAAGVS
                     DEAVEGALARSQDMNLYLIELQERVAAQNRAFTTYSNVLKAKHDTVKNAINNIR"
     gene            complement(931..1740)
                     /locus_tag="PK710_17915"
     CDS             complement(931..1740)
                     /locus_tag="PK710_17915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT11659.1"
                     /translation="MSSKPQGFGKQALRAVMELLAPVIDVLDGVGTTVQLTGQTLYWL
                     MRKPFRVGQMVAMMDFVGVQSLFIVSLTGAFSGMVLALQMIHALRIFQAETMVGGIVV
                     VALTREMAPVFAGLMVTARVGSAMAAELGNMRVTEQIDAITTMSVSPVQYLLTPRMFA
                     AITMMPLLYVVYTIIGTVGAYVVAVMWLNVDPGIFVDKIRQFTVPADIVMGLIKSVVF
                     GFLISAISCRHGFYASGGARGVGMATTRAVVQSAVSILIANYIITSLMTDL"
     gene            complement(1752..2282)
                     /gene="tsaE"
                     /locus_tag="PK710_17920"
     CDS             complement(1752..2282)
                     /gene="tsaE"
                     /locus_tag="PK710_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOT11660.1"
                     /translation="MDERHGTLPMQEIATVELPTRRATTRLGRWLAQGVIAGDLVVLT
                     GGLGAGKTFLTRSVARAMGVGPEIRVTSPTFTLVHEYETVPRLVHADLYRVSDGSEVD
                     QLLLREMRAHGAVLFVEWGEPYLRELGGDALLVKLEMSGPSRAATLVATGPRARQWSS
                     RALPNKLMVRADDKQD"
     gene            2450..4561
                     /locus_tag="PK710_17925"
     CDS             2450..4561
                     /locus_tag="PK710_17925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11661.1"
                     /translation="MQPTDASVVLAPYLEPVIRGRRVAVLGDATDPLGSALLERGARL
                     VHVYDPDASRVAEVLARQGASRTLMTAPLPDGELAVRDGAFDTVVVPNLVLFDPVDTV
                     VARARKLVAAHGYAVFVSPNPDRTASSVERQLSYYELYDLIALQFPEVRMLGQAPFSG
                     YLIADFAPEGDPDVVVDTSLVDRKDQEPKWFVALGTQQARRLDPFTIIQVPGDLAPVA
                     GDEAVTNALQQEVQSLKKELEQTRSQAASLPIVPRVQPRNERVEALEASLREKEEELR
                     RVELRAGDNHVRAGRLENKVRDLEEELRHQRDRAFRLSNDLEEEKKSHTKAELELRMI
                     RSRADMPPVQEGASKVEIDRLRAALGQAETQVATLQSENSTLRDRLTDAEKQGLFLRD
                     SLEDIRTEKLQVEKHVVDLQRALAEKEARIDELDALVVDLQEASGDPELERELDRTRR
                     ELADLQASFDASLQSKLSEVAKLVRERDDARARAEQVRLDKEQRLVSVAQERDTARAS
                     LQASLEKSADLEAALHTLREQLSALQSAASQQHDQTLVEMTQRRDEAVAMLESLRQQQ
                     EQEVEALEHTLRERGQEIQRLQSDVMHHELLVRELVQRLPEVSQAEAQGVTDARRDTW
                     LEESARREGELQQARWKVAELEQRLRDQEERTEVSTEVAELERALFAAQNELDAMRQA
                     LATRPAVPADMAGSGQVPQEK"
     gene            4650..>5628
                     /gene="dnaJ"
                     /locus_tag="PK710_17930"
     CDS             4650..>5628
                     /gene="dnaJ"
                     /locus_tag="PK710_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOT11662.1"
                     /translation="MTQKRDYYEVLGVGREAGPDDIRKAYKQAALKYHPDRNPGDKSA
                     EEKFKEATEAFGVLSDADKRHRYDQYGHAGLEGMGGMDFSGVDIFSHFQDLFSDFFGG
                     FGGGFRGRRSGPSRGADLRVEQQLSLREAVLGCKREISVRAPVACEVCEGSGAQPGSR
                     RETCSTCRGTGQVSNARGFVMFTTTCPTCHGQGSTISKPCEACHGAGQVEKTRKVLVT
                     FPAGIDSGQRLRVPRQGAAGPQGGSPGDLYVDVDVLPDERFERDGVDLLTRVRVSFAE
                     AALGTTVKLDLLDDTELTIDVAAGTQPGDVLTRRGKGVPRLDGAGRGALH"
BASE COUNT         1199 a         1621 c         1663 g         1145 t
ORIGIN      
        1 gatgtcgagg tattcttcca cactgatttc acccgattta agttgttcta gaggcgaggt
       61 tttcagcgtg gcttcggtgg attcgacctt cgataacgaa aactcttcgg gtgagtcgac
      121 gctttgccca gggctcggag cgtccactgc agccgtcgtg ggtgagatga actctccggg
      181 cctgcctgat tttccgattg gattgattcc catctgatgc ctcgtcagta tgccacgagt
      241 ttttctatcg aacgagtcga aggcggagat tgaacgtcat cgcggatcat cggccattca
      301 tcgaatgttg ttgatggcgt ttttcacggt gtcgtgcttg gccttcagca cattggagta
      361 ggtggtaaat gcgcgatttt gagcggctac ccgttcttgt aactcgatca agtataagtt
      421 catgtcctgg cttctggcga gggctccttc gacggcctca tcgctgacac ccgccgcatc
      481 ttgggcaccc gatattggtg cggagccggg gcctgtcacg ccaagacctc cgactcccgc
      541 gggcgagatg cctggcccaa tcgacatacc aaggggccca ggtcgcacgg atgcggccac
      601 aacggggccg gaaggcaaca catgaatcgc cgcggacgct ccgcgaagaa attgctgggc
      661 actccctcgc atgacctcac cgaacgtggg gccatcggga ggcgtaaccc gatgagcgga
      721 aggagtcatc atgacttccg aaggactgga agcaggtcgt acacccatgg tcatagacat
      781 atctgttctc ctgaacgtcg acgcgaacct ggtcgaccga ggttgatatc cacccttcgg
      841 accgtcgcac ccacaagttg cgcacaatct gtattggcgt gaaaacactt cgttttcacg
      901 ggattgacga cgagccccgc gcgcgatggg ctacaagtcc gtcatcaagc tcgtgatgat
      961 gtagttggcg atcaggatcg atactgcgga ctgcacgacg gcgcgtgtgg tggccattcc
     1021 aacaccgcgt gctcctcctg atgcgtagaa gccgtgtcgg catgagatcg ccgagatgag
     1081 aaaaccaaaa acgacggatt tgatgagccc catgacgatg tccgcgggga ccgtaaactg
     1141 gcggatttta tcgacaaata tgcctgggtc cacgttcagc cacatcacgg ccacgacgta
     1201 agcccctacc gtaccgatga tggtgtacac cacatacaga aggggcatca tcgtgatcgc
     1261 tgcaaacatg cgtggggtca gcaagtactg cactgggctc acgctcattg tggtgatggc
     1321 atcgatttgt tctgtgacac gcatatttcc aagctccgcc gccatggcgc tgcccacacg
     1381 agccgtcacc atcaatcccg cgaacaccgg tgccatctct cgcgtcaagg cgacaaccac
     1441 gatccctccc accatggttt ccgcttgaaa tattcgtaag gcatggatca tttgaagcgc
     1501 caacaccatg ccgctgaacg cccccgtcaa cgatacgatg aacaaggact gcacccccac
     1561 gaagtccatc atcgccacca tttgacccac acgaaatggc tttcgcataa gccaatataa
     1621 ggtctgcccc gtcaactgaa ccgtcgtccc caccccgtcg agcacatcga tgaccggagc
     1681 caataactcc atcacggccc gaagcgcctg ctttccaaaa ccttggggtt tgctggacac
     1741 gatggctctt attaatcctg tttgtcgtcc gcgcgaacca taagcttgtt cggaagtgcg
     1801 cgcgaagacc attggcgggc tcgtggaccg gtggccacca acgtggctgc acggcttggc
     1861 ccactcattt caagcttcac caatagggca tcccccccca attcccgaag atacggttct
     1921 ccccattcga cgaacaatac cgctccatgg gctcgcatct cccgcaatag cagttggtcc
     1981 acttccgacc catccgatac gcgatacaaa tcggcatgca cgagtcgagg gacggtttcg
     2041 tactcatgca ccagggtgaa tgttgggctc gtcacccgta tttctgggcc gactcccata
     2101 gctcgcgcga ccgatctcgt caaaaacgtt ttcccggctc ccagaccgcc cgtaagcacc
     2161 accagatccc cggcgatgac cccttgtgcc agccatcgac cgaggcgggt cgtggctcga
     2221 cgggtcggca gttccaccgt cgcaatctcc tgcatgggca gcgtcccgtg gcgttcatcc
     2281 atggctgatt tctacccggt ttttcgtctt atgggtccgt atcgtcccgt tcgggtcctg
     2341 gtgggaccct tatcttccat ctagccttcc aagacagcgt atgcgaaggc atggggcgct
     2401 gctagagtgc gcacgcctca ccattgacca aactgcacgc tggaagatca tgcaacccac
     2461 cgacgcctcc gtcgttctcg ccccttatct cgagcccgtc attcgaggcc gccgcgtagc
     2521 ggtgctcgga gatgccaccg atcctttggg ctctgcgctc ctcgagcgcg gtgctcgatt
     2581 ggtccacgtg tatgaccctg acgcatcgcg cgtggcagag gtgcttgcgc gacaaggagc
     2641 ttcgcgcacc ctgatgacgg caccgttgcc ggacggtgaa ttggccgtgc gcgatggggc
     2701 ttttgacact gttgtcgttc ccaatctcgt cttgttcgac cccgtggata cggtagtggc
     2761 ccgggcgaga aaactggtgg ccgctcacgg ctatgccgta ttcgtatccc ccaatccgga
     2821 ccggactgcc agttcggtgg agcgacagct atcgtattac gaactgtacg atctcatcgc
     2881 cttgcaattt cctgaagtgc ggatgctcgg ccaagctcct ttttcagggt atctcatcgc
     2941 ggatttcgca ccggagggag atcccgatgt ggtggtcgat acctcattgg tggaccgaaa
     3001 agaccaggaa cccaagtggt tcgtggcgtt gggcacacaa caggcgcgtc gtctcgatcc
     3061 gtttacgatc attcaggtgc cgggggactt ggcacctgtt gccggtgatg aagcggtaac
     3121 gaatgcactg cagcaggagg tgcaatcgtt aaaaaaggaa ctcgagcaga cgcggagcca
     3181 ggccgcctcg ttacccatcg ttccccgtgt tcaacccaga aacgaacgcg tggaagctct
     3241 ggaggcaagc cttcgggaaa aagaagagga attgcgacgt gtggagcttc gcgcgggtga
     3301 caatcacgtt cgcgcgggtc gactcgaaaa caaagttcgt gacctcgagg aagagcttcg
     3361 tcaccaacgc gaccgcgcct ttcgtttgag caacgatctc gaggaagaga aaaagtcgca
     3421 taccaaagcc gaactcgaac tacgcatgat tcgcagtcgt gctgacatgc ctccggtgca
     3481 ggagggagcc tccaaagttg aaatcgatcg gctccgtgcg gctctcggac aagcggaaac
     3541 gcaggtcgcg acgctccaat ccgaaaactc gaccttgcgc gatcggctca cggatgcaga
     3601 aaaacaaggc ctgtttttgc gcgattcgct cgaggacatc cggaccgaaa aacttcaggt
     3661 cgaaaaacac gtggtcgatc tgcaacgagc cttggccgag aaagaggcgc gaatcgatga
     3721 actcgatgct ctcgttgtcg atttgcagga ggcttccggg gatcctgaac tggaacggga
     3781 actcgataga acgcgtcgag aactagccga tctccaagct tctttcgatg cctccttgca
     3841 gagcaaactc agcgaggtcg ccaagctcgt tcgcgagcgt gatgacgcgc gcgcgagggc
     3901 cgaacaggtg cggctcgaca aggaacaacg cctggtttcc gtggcgcagg agcgcgatac
     3961 ggctcgtgcc tcccttcaag cctctctcga aaaatcagcg gatctagaag cagctttgca
     4021 tacgttgcgc gaacaactca gcgcgttgca gtccgctgcc tcgcagcagc atgatcagac
     4081 cctcgtggaa atgacgcaac ggcgcgatga agcggtggcg atgctcgaga gccttcgtca
     4141 gcagcaagag caagaagtgg aagctttgga acatacgttg cgagagcggg gccaggagat
     4201 tcagcgtcta caatcggacg tgatgcatca tgaactcttg gtgcgagagc ttgtgcagcg
     4261 tttgcccgag gtgtcgcagg ccgaggcgca aggcgtgacc gatgcgcgaa gggacacgtg
     4321 gctggaggaa tcggcgcggc gtgagggcga gctgcagcaa gcgcggtgga aagtagcgga
     4381 gttggagcaa cgcttgcggg atcaagagga gcgaacggaa gtttcgacgg aagtggcgga
     4441 actggaacgt gcccttttcg cggcgcaaaa tgagttggac gccatgcgtc aggcgctggc
     4501 aacgcggccg gccgtgcctg cggatatggc aggcagcggg caggttcccc aggagaaata
     4561 agtcggttga cagacgagca ggcggactta ctgtgcgcct gaagatcgcg tgagattcac
     4621 gataccggcc gagcgccgga aacgccccca tgacccagaa gcgagattat tacgaggtgc
     4681 tcggcgttgg ccgcgaggct gggccagatg atattcgcaa agcctacaaa caggccgcgc
     4741 tgaagtatca tccggatcgc aaccccggtg ataaaagcgc cgaggagaag ttcaaagaag
     4801 ccacggaggc gtttggagtc ttgtccgacg ccgataagcg gcaccgctac gaccagtatg
     4861 gccatgccgg actcgaagga atgggaggca tggacttttc tggtgtggat atcttctctc
     4921 acttccaaga cctgttttcg gacttttttg ggggcttcgg cggtggattc cggggtcgtc
     4981 gcagcggccc atcacgaggc gccgaccttc gcgtcgaaca acagctttcc cttcgagaag
     5041 ccgtactcgg atgcaaacga gagatctctg ttcgcgcccc cgtggcttgc gaagtctgtg
     5101 agggttcggg agcacaacca ggctctcgtc gcgagacatg ctccacatgc cgtggaacgg
     5161 gacaggtgtc caatgcgcgc gggttcgtga tgttcaccac cacctgccct acgtgccatg
     5221 ggcaaggctc caccatctcc aaaccctgcg aagcctgtca tggcgccgga caagtggaaa
     5281 aaacgcgaaa ggtcttggtg acctttcccg ctggtatcga ttcagggcaa cgattgcgcg
     5341 ttcctcgcca aggagctgct ggtccacaag gtgggtcccc cggtgatttg tatgtcgatg
     5401 tggatgtgct gccagatgag cgtttcgagc gcgatggcgt cgatttgctg acgcgggtgc
     5461 gtgtgagttt cgccgaggca gctctcggaa ccaccgtgaa gctcgatctt cttgatgata
     5521 cggagcttac gatcgatgtg gccgccggaa cgcagcctgg tgatgtattg acacggcgag
     5581 gcaaaggggt accgcggctc gatggggcgg gacgcggtgc gctgcatg
//
LOCUS       DAOSOR010000391         5623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14548_length_5623_cov_4.263649, whole genome shotgun sequence.
ACCESSION   DAOSOR010000391 DAOSOR010000000
VERSION     DAOSOR010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14548_length_5623_cov_4.263649"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1675)
                     /locus_tag="PK710_17935"
     CDS             complement(20..1675)
                     /locus_tag="PK710_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11663.1"
                     /translation="MRTLRTKMPGWLGLAVWLVASTGVGQETPTEAQDAQAPVSSGIT
                     IPLPTIPPPVPTALAPAEPDAIGELGKLLDKLASVQPKERDEAATALAQQDESLLSAI
                     AARLIDLRKNADREAMGVLLEEARKQARKDLKRGESEESEPAAKKDDPTSKSLAAEEH
                     ALIDPKASDWLRYVLQAPAETQPYRDLVAVLGLSRTCVAIGTTASAREIINVYMYFGE
                     MFRIDVERQLARMGDMALPALIEAQYHDSKMVRTWAKRRLDMMGRAIPSEAVRINDNQ
                     ALADVLRAYGRAREVEAVRVIVSFANSDRLQVREAAREAIGQIGEPAQWQLRDAYEQL
                     MGDKPARSWDWKRTAQELFAVYDRARLQEVYTTMDRGLELAKEGKPDQAIELFDRVLA
                     RAPMFERRGEMVPAYLAHARAVRDKDKAAAAAALRRALAIDPSGDQANAVRSELALIE
                     VEELSASGVLDVTLLDRAIELDPSNDRARALRESLKVEVQLEESRWRRYAAAVAIGVV
                     ALAAMVFVALLPRRKKALDTSLPRLPRTPDAPPPPPPKQEELR"
     gene            complement(1699..1998)
                     /locus_tag="PK710_17940"
     CDS             complement(1699..1998)
                     /locus_tag="PK710_17940"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOT11664.1"
                     /translation="MALKRVHLTVRGRVQGVFLEAAAQREARRLGLTGMIVTRHDGSL
                     ELTLEGEEEPLREFIAWSQKGPSAARVEEVDVRWASFSGQFSDVRVLEQDRCQER"
     gene            2091..3350
                     /locus_tag="PK710_17945"
     CDS             2091..3350
                     /locus_tag="PK710_17945"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT11665.1"
                     /translation="MQTRRYPAVVAPFVGMTLLTTALVGCCKNPMEQTKEEAADVGAA
                     PATVSAPPAVAQEDPGPMVTIPAGTLVAGTPCQRIPRITNEELEGINLHLHEFTIDAY
                     PYPNDPAQPPRTGVTRDQAQALCAARGRRLCTELEWERACKGPSNHTYPYGDTFSASK
                     CTGSTTLLRASEQYAACSSAYGVKALYGAVWEWTVSEWGRGGPGGLAAVRGGGHTNPS
                     VRTRCANGQSRSPSETSADLGFRCCGGPINPATVQLSLDKREPLVADVGVDPSLASRL
                     MASLPQDMRKVDGFTPDIDRTWRWHPRDNEEMIVARYRARQDGGRGVFFHPVIFHLCG
                     NSTVRSAKLRGPIAKVFDPTVGTDPQRITLRVETETNQGEVSFTYSYGNVTVQQPEWV
                     KQGNRLDVGADPRIPGVRIRLPGIKRQ"
     gene            complement(3573..3746)
                     /locus_tag="PK710_17950"
     CDS             complement(3573..3746)
                     /locus_tag="PK710_17950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11666.1"
                     /translation="MACLVQTHYAFDDLAEPTRATVGAEGHEVRASARVIVALQAYRA
                     PTMTLTVKLSVTP"
     gene            3972..4490
                     /locus_tag="PK710_17955"
     CDS             3972..4490
                     /locus_tag="PK710_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11667.1"
                     /translation="MNDDSHLPTIRGTLQTIGAVVRGYKIGVTKWMRASGIAGHVWQR
                     NYYEHIIRHEPSLNRIRQTIIDNPSTWASDENNPNRHEAAAGANDDSAAQREWGKGVI
                     GANDMVKAACRACKTNSRVRSTDQRRTSQPHDQQVVAKQHLWNGRSLGAEPSRGGDPR
                     TAASRYVAARIF"
     gene            complement(4466..4720)
                     /locus_tag="PK710_17960"
     CDS             complement(4466..4720)
                     /locus_tag="PK710_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11668.1"
                     /translation="MFRTIRGMLGVMTLRCGVCGSERLSPAYQWVAFQQHFNVPDADK
                     GWLGSKNLVATESVKRVCLDCGHVMGFVSDETLQKIRAAT"
     gene            5359..>5623
                     /locus_tag="PK710_17965"
     CDS             5359..>5623
                     /locus_tag="PK710_17965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11669.1"
                     /translation="MKEIPWKNEGMARMCSRTTLAAAPSLIPRASDPVVPQLSDRPPL
                     AERDHDVGRIIPSFDPATGQTAIAANRKPIAAKRKAIAGRQQAT"
BASE COUNT         1138 a         1713 c         1663 g         1109 t
ORIGIN      
        1 ccaaacccgg cccccccctt taccgcaact cctcttgttt cggtggaggc ggcggcggcg
       61 catcgggcgt gcggggaagc ctgggaagcg aagtgtccag cgcttttttt cgtcgcggca
      121 gcagcgccac gaacaccatc gcggccagcg cgacaacgcc aatcgccacg gcggcggcgt
      181 atcttcgcca cctggattct tcgagctgca cttccacctt gagcgattct cgcaacgccc
      241 gggctctgtc gttggaaggg tcgagttcaa tcgcccgatc gagaagggtg acatccaaca
      301 caccggatgc ggatagctcc tcgacttcga tgagtgcaag ttcgcttcga acggcattgg
      361 cctggtcacc gctcgggtcg atggccaagg cccgacgcaa agcggccgcg gcggctgctt
      421 tgtctttgtc tcgaacggca cgagcgtggg cgagataggc gggcaccatt tccccacgac
      481 gctcgaacat cggcgcgcgg gccaacacgc gatcgaataa ttcgatggcc tgatcaggtt
      541 tgccttcctt ggcgagttcc aaaccacggt ccatggtcgt atagacctct tgcagcctcg
      601 cccggtcgta gacggcgaaa agttcctgcg cggtgcgttt ccaatcccag cttcgggctg
      661 gtttgtctcc catgagctgc tcgtacgcgt cgcgaagctg ccattgcgcg ggttccccga
      721 tctgaccaat ggcttccctt gccgcttcgc gcacctgcag cctgtcgcta ttggcaaacg
      781 atacgatgac acgaacggcc tccacttccc tcgcgcgacc ataggcccgc aacacatcgg
      841 ccagggcctg gttgtcgttg atccgcaccg cttcgctggg aatggcgcgc cccatcatgt
      901 cgaggcgacg cttggcccag gtgcgcacca ttttcgagtc gtggtattgg gcttcgataa
      961 gggcgggcaa agccatgtcg cccatgcggg cgagctggcg ttcgacatcg atgcggaaca
     1021 tctcaccgaa gtacatgtaa acattgatga tttccctggc cgaagcggtg gttccaatgg
     1081 ccacgcaggt gcgagacaag cccaagacgg ccaccagatc ccgatagggt tgcgtttccg
     1141 cgggagcttg cagcacgtag cgcagccaat ccgaggcttt gggatcgatg agggcatgtt
     1201 cttcggcggc cagggatttg ctcgtcggat cgtctttctt ggcagcgggt tcgctttctt
     1261 cggattcgcc tcgtttcagg tcttttcgtg cttgttttcg tgcttcttcg agaagcacgc
     1321 ccatggcttc ccggtcggcg ttttttcgca agtcgatgag ccgagccgcg atggcggaaa
     1381 gcagagactc atcttgttgc gcgagagctg tcgcggcctc atccctttcc ttgggctgca
     1441 cactggcgag tttgtccagg agtttgccca actccccgat ggcgtcaggt tcggcgggag
     1501 ccagcgccgt gggcacgggt gggggaatgg tgggaagcgg tatggtaatc ccggaagaaa
     1561 ccggcgcttg cgcatcttgc gcctccgtgg gggtctcttg cccaaccccc gtggatgcca
     1621 cgagccagac ggccagtccc aaccatccag gcatcttcgt tcgcagcgtt cgcatggtga
     1681 attttctcct cgagatgatc aacgctcctg gcaacgatcc tgctccaaca cacgaacgtc
     1741 gctgaattgt ccgctgaacg atgcccatcg aacatcgact tcctccacgc gcgccgcgct
     1801 gggacctttc tgggaccacg caatgaattc tcgcaagggt tcttcctccc cttcgagcgt
     1861 caattcgaga ctcccatcgt gacgagtgac aatcattccg gtaagcccga ggcgcctggc
     1921 ttcccgttgc gcggccgctt cgagaaaaac accttgaact ctaccgcgaa ccgtcagatg
     1981 aacccgtttc aatgccatac cgtcgcacgg tcctaatccc aggtgcctgg ttgtgcaacc
     2041 atgcgcgcca ccatcgacga ggcaaaccaa cccgacgaga cgaatcatcc atgcaaaccc
     2101 gacgatatcc agccgttgtg gctccctttg ttggcatgac cttgctcacg acagccctcg
     2161 tgggctgctg caaaaacccg atggagcaaa ccaaggaaga agccgccgac gttggcgcgg
     2221 caccagccac cgtctccgcc ccccctgccg tcgctcaaga ggatcccggc ccgatggtaa
     2281 cgattccagc gggaaccctc gtcgcgggca ccccttgtca acgcattccg cgtatcacca
     2341 acgaagaatt ggaaggcatc aaccttcacc tgcatgaatt caccatcgat gcttatccct
     2401 atcccaatga tcccgcacaa cctccacgca cgggcgtaac ccgggatcaa gcgcaagcgc
     2461 tatgcgccgc aagagggcgt cgcttgtgta cggaactcga atgggaacgg gcatgcaaag
     2521 ggccttcgaa ccatacctat ccgtatgggg atacattttc ggccagtaag tgcaccggtt
     2581 cgacgacctt gctgcgcgcc tcggaacagt atgccgcatg cagcagtgcc tatggggtca
     2641 aggcactgta cggcgccgtg tgggagtgga cggtcagcga atggggccgg ggaggccctg
     2701 gcggtctggc ggcggtaaga ggtggcgggc atacgaaccc ttccgtgcga acccggtgtg
     2761 cgaacgggca aagccgttct cccagcgaaa cctcggcaga tctgggattc cggtgctgcg
     2821 gcggcccgat caacccggcc acggttcagt tatctctcga caaacgcgag ccccttgtcg
     2881 ccgatgtcgg cgtcgatcca tcgttggcct cgcgactgat ggcctcgctt ccccaagaca
     2941 tgcgaaaagt cgatggtttt actccggata tcgaccgaac ctggaggtgg catccgcgcg
     3001 acaacgaaga aatgatcgtg gctcgttacc gggcccgaca agacggtggg cgtggcgtat
     3061 tttttcatcc cgtcattttc catctgtgtg gaaactcgac cgtacgttcc gccaagctgc
     3121 gcggacccat cgccaaagta ttcgatccta ccgtgggaac agacccccag cgcatcacgc
     3181 ttcgcgtgga aaccgaaacg aaccaggggg aagtatcctt tacctacagc tatgggaacg
     3241 tgacggttca gcaaccggaa tgggtcaaac aaggcaatcg cctcgatgtg ggcgcggacc
     3301 cacgaattcc cggagtacgg attcgcttgc ccggcatcaa gcgccaatga gcaggagtgg
     3361 cctcgaacgc atgtagactg ggcgtaagcc ccgagccgcg cgtgaaccga aaaagcgcgg
     3421 gcgaccgagg aagtctacaa agtagagcac ccaagcggac aagaacgtcg catccgatgc
     3481 cgtggtggga tttgaagaaa cgctctggca gatggtcaag aagctgaacg gcgacatgga
     3541 cgtggctggg aagaattgga ggggattggg gtttaggggg tgacggacaa tttgaccgtg
     3601 agcgtcatcg tcggcgctcg atacgcctga agggctacga ttacgcgcgc gctggcgcgt
     3661 acttcgtgcc cctctgcccc cacggtcgcg cgtgttggtt cggcgaggtc gtcgaaggcg
     3721 taatgcgttt gaacgaggca ggccatgggg cgacccaatg ctggctagaa atcccaaatc
     3781 attttccgca tgttgtggtc gatgaattcg ttgtcatgcc caatcatgtt catggaattt
     3841 tggtgatcag ccatcctgcc gtcgtcaggg cccatgaaat gggtgccacc ggcgtcggta
     3901 accgggaaca tcacgatgac gcagttaggg cgaatgatga ttcgccccta ccggacccga
     3961 cggatgtgcg gatgaatgat gattcgcacc tgccgacgat acgggggaca ttgcaaacga
     4021 tcggcgcggt cgtacgtggg tacaaaattg gggtgacgaa atggatgcgt gcgtccggca
     4081 tcgcgggcca cgtttggcaa cggaattatt acgaacatat catccgtcac gaaccatcgc
     4141 tgaaccgcat ccgccaaacc atcatcgaca atccttcaac ctgggcgtcc gacgaaaaca
     4201 acccgaaccg tcacgaggcc gccgcagggg cgaatgatga ttcggccgca cagcgtgaat
     4261 ggggcaaagg ggtgataggg gcaaatgaca tggtgaaagc ggcttgcagg gcttgcaaaa
     4321 caaattctcg agtccgctcc acggatcagc gacgcaccag ccagccgcat gaccagcagg
     4381 tcgttgcgaa acagcatcta tggaacgggc gttcgctcgg tgccgaacca tcccgaggcg
     4441 gtgatcctcg caccgcagcg tcacgctacg tcgccgcgcg gatcttctga agcgtttcgt
     4501 cgctcacgaa acccatgacg tgcccgcagt cgaggcacac gcgcttgacg ctttcggtgg
     4561 cgacgaggtt tttcgaacct aaccagccct tgtcggcatc gggcacgtta aagtgttgtt
     4621 ggaaggcaac ccattggtag gcagggctca gtcgttcgct tccgcacacg ccacatcgca
     4681 gggtcatgac accgagcata cctcgaatcg tcctgaacac cgatggaaat tcaaatgcca
     4741 acggcagtca tggcttgctc gaacgtcctt gccgatggtt ggagccctct gctttgggca
     4801 ccacatgctc acgccttcgc gacgcgctcg gcagcacacc cgtgtaccga accaccgggc
     4861 gtgcgacacc caggtgtgat ccaggtttgc cttgatttgg gtccaggacg gcgtgagcga
     4921 cgtatccgct tcatagaccg ggtcatggac aatgccaccc tccgcgtcgt cctggccggg
     4981 ccaaaccaca aacgcaacac acgcagttcc tgggcgaatt tgcggtatcc ggttcgtgcg
     5041 gctcgcgcgg caacccgcat ggcgatggcg tagaaccgcg tgtcatcgcc ctcgacgtgg
     5101 cttcgaagga gcgccttgct ttattccgcg gtggccaagt cgccttatgt tgtagcccgg
     5161 gcaggtgtcc tgacagatcc ccttattttt ccgaattgga aagggatacg agttgaggat
     5221 tcccggatct tgctgttgcc tcggttgttg ctttcccttg ggccaccccc aacagttgcc
     5281 ctgagctatt gtaatgggac tggcgttctg cggtgaacaa tatgggatga gcgtgagcgt
     5341 ttctcgtggg ccgtgaccgt gaaggaaata ccctggaaaa atgaggggat ggctcggatg
     5401 tgctcccgaa cgactcttgc ggctgcgcca tcgctgattc caagagcctc cgatcccgta
     5461 gtcccgcagc ttagcgatcg accgccgctg gcggagcgcg accacgacgt cggcagaatc
     5521 atcccgagct tcgaccccgc aacggggcag actgccatcg ccgcaaaccg caaacccatc
     5581 gccgcaaagc gaaaagccat cgccggaagg caacaggcca ccg
//
LOCUS       DAOSOR010000392         5616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14572_length_5616_cov_3.500989, whole genome shotgun sequence.
ACCESSION   DAOSOR010000392 DAOSOR010000000
VERSION     DAOSOR010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14572_length_5616_cov_3.500989"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            386..709
                     /locus_tag="PK710_17970"
     CDS             386..709
                     /locus_tag="PK710_17970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOT11670.1"
                     /translation="MHRLNKQDRMRLMRFVCSFAWADFEVQKEEKAFIAKLVRQLQLD
                     DDEKQEVELMLQRPPRPEDVDPARVPLKHRELFLDVVRATIAADGMVAPEEAEHFALL
                     QALLK"
     gene            complement(909..2120)
                     /locus_tag="PK710_17975"
     CDS             complement(909..2120)
                     /locus_tag="PK710_17975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11671.1"
                     /translation="MRWKYSLSMFKGSSLYPSMLGVTLLSLVLGGCGSDDEAAGGSSE
                     TAAKAPYGRVSQNTVVRYERPRDDGTTHEATAQVVGEKTIQGKTYWRAKLGDFDSSNA
                     SGTELWVQLPDPHSAVVAGGEFWSRKVLPNPNPSQPSATIELDEPLTLNLDPPVGQPV
                     AVQAKGTIELLGQPLSIDLSGSYTAVSHNASVLTSGGSLHGCHEFSFQGTASNNEFLS
                     VLGVNEISGQAWYHPSFGLVRAVMHIPGKKDYELDFTGADEMGKATTGTNRIQGLRIL
                     GPSETFELNTYAVHGDLDADKDQHAKMLVEARFADDSKAQSSDRPPIDTQIGTTMGVF
                     PHQLVASPISFFHPEENGKGFTFWVGYVDQAAKNESVNGISYHITAKVPNDASNSVRV
                     TSRIIYSLYKP"
     gene            complement(2467..4341)
                     /locus_tag="PK710_17980"
     CDS             complement(2467..4341)
                     /locus_tag="PK710_17980"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HOT11672.1"
                     /translation="MTKSLRWAGVVFWVGIAGVLSCSSEDDSGRVSNAGTGGGTSDAS
                     GSGGDAGTGGTFFTGGSGGGGVVGCVTCSADLRSVVDCETGQVVKECPEDKGCGPDGE
                     CVAPCESASANKSSVGCEYYLVTPDGWSEIPDMDLPYPAGLAPGQASDGSCFAAYVNN
                     TWPTPVDVTVEYNGVVVNAAPHARIPQGAGASLTYLPLPNGQIPPNEMAIVFLSHDGG
                     IGPYKTACPQGITPVFTKDTGQHGTGFGKAIRLATSAPAVVYDIYPYGGAISYITSAT
                     LLLPTSAWNTNYVGVTTDHATRGSQGGEGEIRSPSGITIVAQKDGTKVTLLPSAPIAP
                     RGQVAATPANQAVTYSLNRGELLHFLQVDDLTGTPIEASEPIALWAQHYCMTIPFGVP
                     ACDPGHQQIPPVSATGSQYAAVRYRNRIPSGPEEAVPWRFVGMVDGTVLTYDAGFDPV
                     AAGAPTTLQQGQLVTFNHTGPFVVRSQDDSHPFYVAAHMSGGDPFDGIGDPETVNIIP
                     TAQWLKQYIFFADPTYGNSNLVVIRKKGPEGFAPVTLDCLGPLDGWTALGEFEYTRVD
                     LQANGTPVGACDNGRHVMESDIPFGLTVWGFDQYVSYAYPAGASVKPINTVKVPPVPK
                     "
     gene            4717..5553
                     /locus_tag="PK710_17985"
     CDS             4717..5553
                     /locus_tag="PK710_17985"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOT11673.1"
                     /translation="MLRKIATKGMVVAVLLATSCVEEPNNPDYWSKSSHDAGTDSLQD
                     SGSNANHDVNSEADGSQDFDAQHDSPVDSADSSTLPDDSAVTEPDATIHVPCTKVAHL
                     GDSLTAYTIDELKDAYAAVGIEATIEAYGGRAIYEYCCGDDTTGKQAAERLVASGFAG
                     CWVIALGTNDTANVSAGAAISRADRIDAMMKTIDDSAQVRVMWVNTYTTKTEGHWSNA
                     NMELWNAELEKARARWPNMHVFDWASIAKTGSAPFSDGIHHTTQGYAVRNAAIAKAAS
                     EL"
BASE COUNT         1240 a         1607 c         1543 g         1226 t
ORIGIN      
        1 agacggtgtc gaccatccgg cggatcgcag cctccacctc ggccttcgcc gccgtatcca
       61 ccgcaggctg agcgcttgcc gccatcgccc acaacgacac aacccatagg ccaacagcaa
      121 acacgacagt tgacacgggg tgcctaagca taccactacc cttatcattc gttgtcgaag
      181 gggtcgaccg tgttggatgt tggggctaag gtgggattgt ctcatgaatt gtacgatgct
      241 tcatgatttg ccgggggaac caatgtcagg tgttcgttgg gcaatcgctg gaagtaccac
      301 cgattcgctg ctggattggt tggatggttg accatgcccc tgtccccctc tttcgaagga
      361 cttcatccac tgctacggtt ggcccatgca tcgcttgaac aagcaagacc gaatgcgact
      421 catgcgcttt gtttgctcct tcgcctgggc ggatttcgaa gtccaaaaag aagaaaaggc
      481 cttcatcgcc aagcttgtcc gtcaactcca actcgatgat gacgaaaagc aagaagtgga
      541 gttgatgttg caacgaccac cgcgccctga agacgtcgac cccgcgaggg ttcccttgaa
      601 acaccgcgaa ttgttcctgg atgtggtgcg tgcaacgatt gctgccgacg ggatggtggc
      661 accggaagag gccgagcact tcgcgttgct ccaggcattg ttgaagtaat cgatcaaggt
      721 gtcacaacgt tgggcaaggg tcttgaactg gtttgcttgt caaggctcga gcgttcgtcc
      781 atccacgata ttgtccctac gcaaaccccg tcgcggacaa cgacgacagc aaggcgccct
      841 gccggaaaac actcggtgac aatgggcatc gcccgtgccg cgtataccgt cgaaaaacgc
      901 ggttggcatc aaggcttgta gagcgaatag atgatccggc tcgtcacccg caccgaattc
      961 gatgcgtcgt taggcacctt tgccgtgata tggtaggaaa taccgttgac cgattcattt
     1021 ttcgccgcct ggtctacgta tcccacccaa aacgtaaagc ctttgccgtt ttcttccgga
     1081 tggaaaaagc tgatggggga tgcaacgagt tggtgtggaa agacgcccat cgtcgttccg
     1141 atctgtgtgt cgatgggtgg acgatccgag gattgcgctt tcgaatcatc cgcaaaacgc
     1201 gcttccacga gcattttggc gtgctggtcc ttgtcggcat cgaggtcgcc atgcaccgca
     1261 taggtattga gttcaaacgt ttcagaaggt cccaggatac gcagaccttg aatacggttg
     1321 gtgccggtgg ttgcttttcc catttcgtcg gcgcccgtga agtcgagttc atagtccttc
     1381 tttccgggaa tgtgcatcac agcgcgcacc aggccgaaac ttggatggta ccaggcttgc
     1441 ccgctgattt cgttgacccc aagaacactg agaaattcgt tgttcgatgc cgtgccctgg
     1501 aacgaaaact catgacatcc gtgcaaggaa ccaccggaag tcaagacgct ggcgttgtga
     1561 ctaacggcgg tataagagcc ggaaaggtcg atggaaagag gctgccccaa aagctcgatc
     1621 gtacctttcg cctgtacggc aacgggctgt ccgaccggag gatcgaggtt gagcgtgagc
     1681 ggttcgtcca actcaatcgt ggcagaaggt tgggatggat tgggattggg cagcactttt
     1741 cgcgaccaaa attcacctcc cgccacgacc gccgaatggg ggtcggggag ctgaacccaa
     1801 agctccgttc cgctggcgtt cgatgagtca aagtccccaa gttttgcacg ccaataggtt
     1861 tttccctgga tcgtcttttc tcccacgacc tgcgccgtgg cttcgtgagt agtcccatcg
     1921 tcacgtggtc gttcgtatcg tacgaccgta ttttgagaaa cacgaccata gggcgccttg
     1981 gcggctgtct ccgagcttcc ccccgctgct tcatcatcgc tgccacaccc cccgaggacc
     2041 aaagacaaca acgtgacgcc aagcatggaa ggatacaacg agcttccctt gaacatcgaa
     2101 agactgtatt tccagcgcat cgcaagccat tccttatatg ttcgaacgtt ttccgatcct
     2161 gcagactagc aaaacccgct cgcgtttaca cgacaaaccc atcctcaacc caaggagcga
     2221 cctcaactcc cctcggccga aggcctcgta gggcataggt gcctgttatt acacgatttt
     2281 tacgctcacc cctcgatcgt tttcctccca tatctcacca ctagcagcgc ggcgctacca
     2341 cggtaaggat agaatccgtg tagcgcaaag ctcctttcgt cgaagcgaat atgcgaatat
     2401 ttcgatgact caccatcgaa tggacggtgt tgttggcggg cgcgttggat acgctaacgc
     2461 tgacgcctat ttcgggacgg gaggtacttt cacggtattg attggcttga cactggcacc
     2521 cgcggggtag gcataactca cgtattggtc gaatccccag acggtaagtc caaaaggtat
     2581 gtcgctttcc atgacatgac gaccattgtc gcaagcgccc acgggagtgc cgttggcttg
     2641 caggtcgacc ctcgtatatt caaattcccc aagcgcggtc caaccatcga gagggccgag
     2701 acaatcgagg gtaaccggcg cgaaaccttc ggggcctttt tttcgaatca cgacgaggtt
     2761 cgagttgccg taggtgggat ccgcaaagaa gatatactgc ttgagccact gcgctgtagg
     2821 gatgatattg acggtttcgg gatctccgat accatcgaat gggtcgccgc ccgacatatg
     2881 cgcagccaca taaaatggat ggctgtcgtc ttggctgcga accacaaaag gtccggtatg
     2941 gttgaacgtt acgagttgtc cttgttgtag cgtggttggc gccccggccg ccacagggtc
     3001 aaaacccgca tcgtaggtaa ggaccgtacc atccaccatt cccacgaaac gccatggcac
     3061 agcctcttca gggccggagg gaatacgatt tcggtaacga acggccgcgt attgcgagcc
     3121 ggttgccgac acgggaggga tttgctgatg gccgggatca caggctggga ccccgaacgg
     3181 gatggtcatg caataatgct gcgcccacaa ggcgataggt tccgaggctt caatcggagt
     3241 ccccgtcaga tcgtccactt gaagaaaatg caatagttcg ccccgattga gggaataggt
     3301 caccgcttga ttcgctggag tggccgcgac ttgacctcga ggagcaatag gagcggaggg
     3361 tagcaaagtg accttggtac catctttctg tgccacgatc gtgatgccgc tgggtgagcg
     3421 tatttcgcct tcaccgccct gagatcccct tgtcgcgtgg tccgtggtca cccctacata
     3481 attcgtattc cacgcactcg tcggaaggag taacgtcgcg ctggtgatat agctaatcgc
     3541 tcccccataa ggataaatat cataaacgac cgccggggcc gacgttgcca gtcggatcgc
     3601 ttttccgaat cctgtgccat gctgtcccgt gtccttggtg aaaacaggag taataccttg
     3661 tgggcaagcg gttttgtagg gccctatgcc accatcgtgg ctcaaaaaca cgatggccat
     3721 ctcgttgggc gggatctggc cgttgggtaa gggaagatac gtcaacgaag cgcctgcacc
     3781 ttgcggaatc cgagcatggg gtgccgcgtt caccacaaca ccattgtatt cgacagtgac
     3841 atcgaccgga gttggccagg tgttgttcac gtacgcggca aagcaactcc cgtcgctggc
     3901 ctggcctggc gctagtccag caggataagg aagatccata tcgggaatct cactccagcc
     3961 atcgggagtc acaagatagt actcacaacc cacggagctt ttgttggcac tcgcgctttc
     4021 gcagggcgcc acacactccc cgtcgggacc gcaacctttg tcttccggac actctttgac
     4081 cacttggccg gtttcacaat ccaccacaga tcgaagatcc gccgagcaag tgacgcagcc
     4141 gacaacccct cctcccccac taccacccgt gaaaaatgtc ccccccgtgc cagcatctcc
     4201 tccggagcca gaagcatcgg aagtcccgcc gcccgtcccc gcattggaaa cgcggccact
     4261 gtcgtcctcc gaagaacaac tcagcacacc cgctatccct acccaaaata ctacgccagc
     4321 ccatcggagc gattttgtca ttcagcaatc gtacccttta tcaagctcct ctcaagcagc
     4381 ttttcatgag ccgaagcatt ttttgagact ccctggcaac cgcataagcc tgccacccaa
     4441 agcggcagag ttacgtcgtc ttgatgagac tatgtgctga attttattac agatatttct
     4501 tccccgggcc cgcttgcttc gacgctcttt tccattccgc aaacattccc caatgtcctc
     4561 acgcaacgga tccgttgcac gtctcctttg cggttggtac gaatgggtga gaatgtgccc
     4621 gcaaggaggt gcgatgccct tacggttctg gcatccgata cgcggcccga cgctgattat
     4681 tcgcgccggt ccaggtcgct catgccatac tcatgcatgc ttcgaaagat cgccacgaaa
     4741 gggatggttg tcgccgtgtt gctcgccaca tcgtgtgtcg aggagccgaa caaccccgat
     4801 tactggtcca aatccagcca tgacgcgggt acggattcgc ttcaggatag tggatccaac
     4861 gcgaatcatg acgtcaattc cgaagccgat ggctcccagg atttcgatgc gcaacacgat
     4921 tctccggttg attcggccga ttcgagcacc ctaccggacg atagtgcggt taccgaaccc
     4981 gacgcgacca tccatgttcc atgtaccaag gtcgcgcatc tcggcgatag ccttacggcc
     5041 tatacgatcg acgaactgaa agatgcgtat gccgccgtgg gtatcgaggc tacgatcgag
     5101 gcctacggtg gccgtgcgat ctacgagtat tgctgtggcg acgacacgac tgggaaacaa
     5161 gccgcggaac gtttggtggc gtccggattc gcggggtgtt gggtgattgc tcttggcacg
     5221 aatgataccg ccaatgtgtc ggcgggagcg gcgatttccc gcgccgaccg gatcgatgcc
     5281 atgatgaaga cgatcgatga tagcgcgcag gtcagggtga tgtgggtcaa cacctatacg
     5341 accaaaacgg aggggcattg gtcgaacgcg aacatggaat tatggaacgc agaactcgag
     5401 aaagctcggg cgcgttggcc gaatatgcat gtgtttgatt gggcgagcat cgcgaaaacg
     5461 ggaagcgcgc cgttcagcga cggtatccat catacgaccc aaggctatgc cgttcgcaac
     5521 gccgcgatcg ccaaagccgc gagcgaactc tgagaaaaat cccgtcgtgt cggccgtttc
     5581 gtggttgaat ccgcgggcaa ggctctcggt tcgtct
//
LOCUS       DAOSOR010000393         5611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14580_length_5611_cov_5.008819, whole genome shotgun sequence.
ACCESSION   DAOSOR010000393 DAOSOR010000000
VERSION     DAOSOR010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14580_length_5611_cov_5.008819"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /gene="fbaA"
                     /locus_tag="PK710_17990"
     CDS             <1..839
                     /gene="fbaA"
                     /locus_tag="PK710_17990"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF006628.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOT11674.1"
                     /translation="VIGAVAAAQHAHLVAEHYGICVAMHTDHANKKLIPWVEALIGYS
                     EKHFEKTGRPLFSSHMLDLSEETLEFNLGECARILPRMTKLGMSLEFELGVTGGEEDG
                     VGQDLESIDNSKLYTQPQDVLRAYEKLNKLGHFSIAASFGNVHGVYKPGNVKLRPEIL
                     EASQSLVQKTHNTGKNPLHLVFHGGSGSEKEKIHQAVSYGVFKMNIDTDTQFAFSQPI
                     GAYVEKNPKAFKVQVDPDDGTPYKKFYDPRKYLRAAEQGIVERLQEAFADLGSVGKTL
                     VG"
     gene            complement(898..2742)
                     /locus_tag="PK710_17995"
     CDS             complement(898..2742)
                     /locus_tag="PK710_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HOT11675.1"
                     /translation="MRSAEPAEPAEPASVSEQPSSQDNTDPTQEQSQVRAIQGLSSLP
                     LAEPVLPVHPRTQPLEISSTRVRSYRFIRAYLTTFQVCFSYLWITLGGKLMGPTWMLQ
                     RMEAAHPRNAKRVERTIVALQGLFIKVGQLLSIMANFLPEAFRAGLQGLQDSVPPRPF
                     DQIAERIQIEFGKPVDEVFDRFERNPIASASLGQVHEAYLKDGTHVAVKVQHKDIDEV
                     CRLDLKTIRRIMQIVSFFFPIQGLEVSYRQIREMIGRELDFNGEASSIERISSHFAND
                     PNVGFPLVIRTHSTGRVLTTTFVEGIKIGDVARLERAGINRTELAQRVVRTYCQMIFV
                     DGMYHADPHPGNMLVTRTGKLILLDFGAVAVLSNEMREGIPEFLEAALRRDTDRLIRS
                     MRKMGFISKRSDAEVSEKVIEYLHHRFQEEIKLESFNLKDIKVDPQRGFENLIDLHRM
                     NIGMKELSGSFQVPQDWVLLERTLILLTGVCTNLDPNMNPMDVIRPYLKEFVLGNRDW
                     TEIAMEAAKDMAMRAVSLPEDLHKYLSKAVRGELEVRVRDVRDGARLVYAATRQLIYA
                     LTGIAFGISGLQLYLAGDTVLALGCFGGVGTMATLFVASTIWIRISRR"
     gene            3177..3731
                     /locus_tag="PK710_18000"
     CDS             3177..3731
                     /locus_tag="PK710_18000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11676.1"
                     /translation="MGRKRGLIWASVVVLVGLGAWSCSDDSGGNGKASKKELDWFTSS
                     CADLGGVETPAGACFIGCKTNDDCGLPTLRCTGTAAWSVGQCEISYEIAQTRGCGPKG
                     WHQRSYGCYMQCFGGNDDECPSGFRCLKDSIHAGDYFCSGYSGGSSSDCSVPCERGCC
                     SPTGRTCCEPPFCSGICSGSPCCR"
     gene            3789..4757
                     /locus_tag="PK710_18005"
     CDS             3789..4757
                     /locus_tag="PK710_18005"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOT11677.1"
                     /translation="MRAEDEKGLSVLAPASMESRYDGVMRGARMAWILFFFALMGCKN
                     TAKPAHIPSALPTVVTSPQEQSLEREMFERVNQERQKHGLAALQYDERLAAIARFHSQ
                     DMRETGFFGHQSPSTGSPQDRIDKAGYVAAESRENVALAPDVDTAHRNLLASPGHYAN
                     IVATTVTHLGVGVVQDKKIPGQVQGYFFTQLFAKPVTLLTPEQARAQILRQVSDARRQ
                     SRLAPLPLHPALERFAKDNVQTIDARAPGRSLESINQRALALLQQERGLTFRTIEASA
                     QAALGAEFLNTRDLLDPTVRAVGFALATGVDAQGKSVLTMLILLGR"
     gene            complement(5294..>5611)
                     /locus_tag="PK710_18010"
     CDS             complement(5294..>5611)
                     /locus_tag="PK710_18010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11678.1"
                     /translation="GLDYTEMFQLMAEGVVKGSEVPCEFMIPEPPPGETLDLNTVEVA
                     YKSNGTLVEVFNKVASEEECDSSSFLIDGERIVLCPQACDVVQADADAKIDILFGCEI
                     QIK"
BASE COUNT         1241 a         1592 c         1558 g         1220 t
ORIGIN      
        1 aggtcatcgg tgccgttgcc gctgcccaac acgcgcatct cgtggcggag cattacggca
       61 tctgtgtggc catgcacacc gatcacgcga ataaaaagct cattccgtgg gtggaagcgc
      121 tcatcggcta cagcgagaaa catttcgaaa aaaccggacg tcccttgttc agctcccaca
      181 tgctggactt gtcggaagaa acactcgagt tcaatctcgg cgagtgtgcg cgaatccttc
      241 cacggatgac caaactcggc atgagcctcg aattcgaatt gggcgtcacc ggtggcgagg
      301 aggatggcgt tgggcaagac ctcgaatcca tcgacaattc caagctgtat acccagccgc
      361 aggacgtgtt gcgcgcttac gaaaaactca ataaactcgg acacttttca attgctgcct
      421 cgttcggcaa tgtgcatggc gtgtacaagc caggcaatgt gaaacttcgt ccggaaatcc
      481 tcgaagcctc ccagtcgctc gttcaaaaaa cgcataacac gggaaaaaat ccgctgcatc
      541 tcgtgttcca cggtggctcg ggttccgaga aagaaaaaat ccatcaagcg gtatcgtacg
      601 gcgtgttcaa aatgaacatc gatacggata cccaattcgc tttctcgcag ccgatcggcg
      661 cttatgtcga gaaaaatccc aaagcattca aagtgcaggt cgatcccgac gatggcacgc
      721 cgtacaaaaa gttctatgac ccgcgcaagt acctgcgcgc cgcggaacaa ggcatcgtag
      781 aacgcctgca agaagccttc gcggaccttg gttccgtggg caaaaccctc gtcggatagc
      841 gctttccccc ttccttccca tcgcccctcc tcggtaaata accgggtccg tccgggctca
      901 acgtcgagaa atccgtatcc agatcgtcga tgcgacaaat agcgtggcca tggttcccac
      961 cccgccgaag cagcccagcg ccaataccgt atcccctgcg agatagagct gaagaccgga
     1021 gatgccgaag gcaattcccg tgagcgcata gatgagttgc cgggtggcgg catacaccag
     1081 acgtgccccg tctcgaacat cgcgcacccg cacctcgagt tctccccgaa ccgctttcga
     1141 aaggtacttg tgcaaatctt cgggcaggga caccgcccgc atcgccatgt ctttcgccgc
     1201 ctccatcgca atctcggtcc agtcccgatt tccgagcacg aactccttga gatacgggcg
     1261 aatcacgtcc atgggattca tgttcggatc gagattcgtg catacacccg tcaacaaaat
     1321 gagggttcgt tccagaagca cccaatcttg cggtacttga aatgaacccg acagctcctt
     1381 catcccgata ttcatccgat gaagatcgat caggttttcg aagcctcgtt gtggatcgac
     1441 tttgatgtcc ttgaggttga agctctcgag cttgatctct tcttgaaatc gatgatgcag
     1501 gtattcgatg accttttcgc tcacctccgc atccgagcgt ttggagatga aacccatctt
     1561 gcgcatggac cggatgagcc ggtcggtgtc ccgtcgaaga gccgcttcga gaaactcggg
     1621 aatcccctcg cgcatctcgt tggacagtac ggccaccgct ccaaagtcca ggagaatcaa
     1681 cttccccgta cgcgtcacga gcatgttccc gggatgagga tcggcgtggt acatgccatc
     1741 aacgaaaatc atctgacaat aggttcgcac gacccgttgc gcgagttccg ttcggttgat
     1801 gccggctcgt tcgagacgcg ccacgtctcc gatcttgatt ccttcgacga aggtggtggt
     1861 caacacgcgg cccgtggaat gcgtgcggat gaccaaggga aatccaacgt tcgggtcgtt
     1921 ggcaaaatgg gaggaaatcc gttcgatgct gcttgcttcg ccgttgaagt ccaactcccg
     1981 cccgatcatc tcgcggattt gccggtaact cacctcgagg ccctggatgg ggaaaaaaaa
     2041 gctgacgatc tgcatgatgc gacggatcgt cttgaggtcc agacggcaca cctcgtcgat
     2101 gtctttgtgt tgcaccttga cggcaacgtg ggtgccatcc ttgagatagg cttcgtggac
     2161 ttgtcccaaa ctggcgctgg cgatgggatt gcgttcaaaa cgatcgaaga cttcgtcgac
     2221 cggtttgcca aattcgatct gaatccgttc ggcaatctga tcgaaaggac gaggcgggac
     2281 gctatcttgt aggccttgga gccccgcgcg aaacgcttcc ggaaggaagt tcgccatgat
     2341 gctcaacaac tggccgactt tgatgaagag cccttgtagc gcaacgatgg tgcgttcgac
     2401 tcttttggca tttcgcggat gagccgcctc cattcgttgc agcatccacg tgggccccat
     2461 gagtttgccg cccagggtga tccataaata actgaaacaa acctgaaaag tcgtcagata
     2521 ggcgcgaatg aaacggtagc tccgaacacg ggtgctcgaa atctcgagcg gctgcgtgcg
     2581 tggatgcacc ggcaacaccg gctcagccaa aggcaatgac gacagaccct ggatcgcgcg
     2641 aacctgcgat tgctcttgcg tgggatcggt attgtcttgg gatgacggtt gctcggaaac
     2701 cgaagcgggt tcagcaggtt cagcaggttc agcggatcgc acccccacag cgtagcacga
     2761 ccgcaaccca tcctgtgaat cgtcgatttc gccttcgaga gccttgcatg actccgatca
     2821 gtccaagaac tgaatcaagg gaaaagcttg cgatccgatg atgaataccg aacgctcgtc
     2881 ttggctgggg gcgtgatacc gacagacgct gatgagcgaa ggcctctctc ctggcaattg
     2941 acacctttgg atccgtatgc ggatagttcg taaaaactct ttataaacaa gtggttgcgt
     3001 tattccgatg ccatcctccc cgtgtcgatg gcgcacgtcc catggtcttt gcacctacgg
     3061 cttgacagtg gcgtgggtgg cggagacact gccctgccat aagagcctgt ttggggcagc
     3121 gccaacgatg cagggcagtc gtgtcggctt ccgtggaggc atgagaggaa ggtgtcatgg
     3181 ggcgaaaaag gggattgatc tgggcatcgg tcgtggtgct ggtgggtctt ggtgcatggt
     3241 cttgctccga cgattccggg ggcaatggga aagcctccaa aaaagagctt gactggttca
     3301 cctcatcctg tgccgatctg ggtggtgtcg agactcctgc gggcgcgtgt ttcattggtt
     3361 gcaagaccaa tgatgactgc ggtttgccga cgctgcgatg cactggcacc gctgcctggt
     3421 cggttggaca atgcgagatt tcttacgaga tcgcccagac gaggggttgc ggtccgaaag
     3481 gttggcacca gcggtcctat ggttgctata tgcaatgttt tgggggcaac gatgacgaat
     3541 gcccatcggg atttcgctgc ctcaaagaca gcatccacgc gggtgactat ttttgcagcg
     3601 gctattcggg cggcagtagc agcgactgca gcgtaccgtg cgagcgcggc tgctgcagtc
     3661 ccacgggccg tacatgctgt gagccgccgt tctgttcggg gatctgctcc ggttctcctt
     3721 gctgtcggtg acacccatcg aaagcctggt cgcgtgaccc atctacgttt tgtattcgat
     3781 ggcgatgctt gcgagccgag gatgaaaaag gattgtccgt cctggctccg gcctccatgg
     3841 aaagccgcta tgatggggtg atgcgtggcg ctcgtatggc atggatcctg ttcttttttg
     3901 ccctgatggg ttgtaaaaac acggcaaaac ctgctcatat tccttcggct ttgcccacgg
     3961 tcgtcacctc accgcaggag caaagcctcg agcgtgagat gttcgagcgc gtcaatcagg
     4021 aacggcaaaa acatgggctg gcagccttgc aatacgatga acgattggct gccatcgcgc
     4081 gctttcattc ccaggatatg cgcgagacag gctttttcgg ccatcaatcg ccgtcgaccg
     4141 gctcgcccca ggaccgcatc gacaaggcag gatacgtcgc cgccgaatcc cgagaaaacg
     4201 tcgctcttgc cccggatgtg gacacggcac atcgaaacct gctcgcaagc ccaggacatt
     4261 acgccaatat cgtcgcaacc accgtgactc atctgggggt cggcgtggtg caagacaaga
     4321 aaatccccgg ccaagttcag ggatattttt tcacgcaact gttcgccaaa cccgtgaccc
     4381 tcctgacgcc tgagcaagcg cgggcgcaaa ttctgcgtca ggtgtccgac gcgcgacgcc
     4441 agtctcggct tgcaccgctg cctttgcatc cggcgttgga gcgatttgcc aaagacaacg
     4501 tgcaaaccat cgatgcccgc gcgcccgggc gaagcctgga gtcaatcaat cagcgagcgc
     4561 tcgccctgct ccagcaagag cgtgggttga cgtttcgaac catcgaggcc tccgcgcaag
     4621 ctgctttggg cgccgagttc ctgaacaccc gggacctgct cgatcccacc gttcgtgccg
     4681 tcggatttgc gttggccacg ggcgtcgatg cccaaggaaa atccgtgctg acgatgctca
     4741 tccttcttgg gcgataaagc cgtcgtggga ttgccaagac cctggaaacg acccgggatt
     4801 gacctcagcc ctcccctcat tttccccggg cttttccttc acggttacgg ccacggtcac
     4861 ggctacggct acggcgcacg gtgcatggcg cacaataacg gtcacgttca tcccatatcg
     4921 ttcaccgcaa aacgccagta caatgtttag tagcccaggc caactgttgg gggtgctcca
     4981 agggggccaa ggatcgagcc aaactcaaga cccaagaatc ctaaactcgt atccctttcc
     5041 aattcaggaa aaaatgaggg aatctgtcag gtttcgtagg ggtgtaccat gctacgcgtc
     5101 aatacttgaa gtatgctgtt gttctcgacg aaaagagtcg acctatattg ctttacaatc
     5161 ccaaacgatc cgcgtcgtac agcgtgctta tctccgaaga tggctcgata ttgcgcaaac
     5221 atgatgacaa tcagtcggaa aaagtgactg cgatcgagcg gcgcgtttcg gaagttgtcg
     5281 ggcggtgacg tgactacttg atttgaatct cacagccgaa aagaatatcg atctttgcgt
     5341 cggcatcggc ctggacaacg tcacaagcct gcggacataa tacgattctt tctccgtcga
     5401 tgaggaacga cgaagagtca cattcttcct cggatgcaac cttgttgaag acttcgacaa
     5461 gggtgccatt ggacttatac gcaacttcca cggtgttcag gtccagcgtt tccccggggg
     5521 gaggctccgg gatcatgaat tcacagggga cttccgagcc cttgacgact ccctcggcca
     5581 tgagttggaa catttccgtg taatccagcc c
//
LOCUS       DAOSOR010000394         5609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14587_length_5609_cov_3.716421, whole genome shotgun sequence.
ACCESSION   DAOSOR010000394 DAOSOR010000000
VERSION     DAOSOR010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14587_length_5609_cov_3.716421"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="PK710_18015"
     CDS             complement(<1..871)
                     /locus_tag="PK710_18015"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOT11679.1"
                     /translation="MRPVIVFVAMVCTMLGCEKHPPSDVQTQKTVASVTSKEQAKPAI
                     SLASAASSSSVSEEIRNKEGRASGKVREFTLRFDDTPVGPMQAVVVVPREASPHHRLP
                     VLIAMHGQGEALKGPAKGARGWVDDYWLGKALRRLNAPPLTNADFRHRFSEKRLDMLN
                     QSLFAQPFRGLIVVCPYTPHKYLKGKEAFSQSLLADFIVDTLLPQVYEQAPAMGTPAS
                     TAIDGVSLGGRASLLVGLARPEAFGAIGALQPAFDGEDAPELAKRARNALKKNPSLTV
                     RLLTSDNDYYLEST"
     gene            961..1773
                     /gene="nadE"
                     /locus_tag="PK710_18020"
     CDS             961..1773
                     /gene="nadE"
                     /locus_tag="PK710_18020"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001979.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HOT11680.1"
                     /translation="MNPQQERIAQALHVQSVIDAKVTISEIVRFLSSYLIRSGARGYV
                     LGISGGQDSTLCGKLAQLAVEDARHHSALPISFWAMHLPYGKQRDEQDVKLALSFIEP
                     DRLVTVNMQASVDASVRALEDGLGEPLSDYHRGNVKARERMVMQYALAGSHNLLVLGT
                     EHAAEAVTGFFTKYGDGACDLAPLVGLTKRQGRLLLQSMGAPARLYEKVPTADLEDLR
                     PLYPDEASLGMSYEQIDDYLEGKSVDAQIAKRLEAIYVATKHKRAMPVTRFG"
     gene            complement(1777..3513)
                     /locus_tag="PK710_18025"
     CDS             complement(1777..3513)
                     /locus_tag="PK710_18025"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT11681.1"
                     /translation="MVSQSDSKDDLTREPPLRPVRMRLDPGNLQLGKYRILGEVGRGG
                     MAEVFLAVAQGLSGFNKLVVLKIIRAEVAEDPDARSMFVDEARLAGRLNHPNVVHTLE
                     VSEDAGRPILVMEHLDGQPLSSILRRCQRDEKPMPMAMGLRVLAEALQGLHYAHTLKN
                     FDGTPLRLVHRDVSPQNIFVTFDGQIKVLDFGIAKAAISSSETRAGVMKGKAAFMAPE
                     QVSGESINHRADIYSTGVVLWQLVTGQQWWKGKAQGQILLTVLNGDVPKPSDVCENVD
                     ADLEAICLKAMEVDPSKRFQSALEMHDAIEAAIDARSSVSTRDVGAFVANLFKDEREM
                     LSRTLETELKSLSESGYRTQTGTLSHPHSEDSQSSIRSVNSDASLSGMRLSRRPEKRK
                     GAAVIMAVVGLAILAGATALLLRTPPETVASAEQMSAPSEPSNPPPPALSLEATKPMV
                     PAASCTIAVKAIPSNATISIDGKPMKDNPSTLHVVQDGKVYSIHAHAPGFVPRTVEVA
                     ALRDASFVVELEPVRTGGVVPRVLDPKAPEPVVQEQPPASPPEAKPPAEKKEDPKSAR
                     DRIKELDTTSPW"
     gene            3744..4397
                     /locus_tag="PK710_18030"
     CDS             3744..4397
                     /locus_tag="PK710_18030"
                     /inference="COORDINATES: protein motif:HMM:NF016261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HOT11682.1"
                     /translation="MMHDSTYKAPSVDHLGFLDHPEDWTEDFARQTAPIVGITQGLTD
                     RHWAVIRYLRSQFEKTGRCPLLYFVCSDNGLSVADLQALFPSGYLRGACRLAGISYRD
                     SILCSPCHGKDDRQCTAKGIDKVYRVDVLGFLVDPDEWDSAFAIHRAREMGIEAGLTQ
                     QHWEVLDHLRNVYDETGDVPTVYSVCEELGLSLETLAQLFPSGYHRGAVKLAGLRVH"
     gene            4440..>5609
                     /locus_tag="PK710_18035"
     CDS             4440..>5609
                     /locus_tag="PK710_18035"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOT11683.1"
                     /translation="MENRIGALHGSAMFSQASTRILVTVVLLITGCERGKTTDPEPTA
                     TSSSPAVAASSTPIPVETPKKPLNVILLVVDAMRYDMPWEGYDRAIAPNLTQLHAKSI
                     AYERGYAISSFTSKSVGGLLTGRYPSSLARTSPFFTSYKDENEFMAELLQKRGIRTLG
                     VQAHMYLKSVAGFHQGFDVWKMVPGIQWDYNKDPYITAPDHTKLINEIHAERENTSGQ
                     FFAYYHYMDPHDVYNTHSIAPDFGKKGRDRYDQEIWFTDHHIQAMLDYVNTQPWGKHT
                     VVIVTGDHGEAFGEHNFWKHAFELYEVLIRVPLFIYVPGLEGRKISRWRSHIDLVPTI
                     LDMMGIPIPKDLPGVSLWPEIQGKETPARPIIADLPADTYNVRKRVFIDENGYKLS"
BASE COUNT         1277 a         1504 c         1516 g         1312 t
ORIGIN      
        1 gcgtggactc gaggtaatag tcgttgtcgc tcgtgagcaa tcgtacggtg aggctcggat
       61 tttttttcaa tgcgtttcgc gctcttttcg ccaactccgg agcgtcttcc ccatcgaaag
      121 ccggctgtaa tgcaccgatc gccccaaacg cctcaggccg agccaatcca accagcaggg
      181 aagcgcgccc ccccaaacta acgccatcga tggccgtgga ggctggcgtt cccattgccg
      241 gagcctgttc atatacttgg ggcaatagcg tatccacgat gaaatccgcg agcaaagact
      301 gagagaacgc ttccttgcct ttgaggtact tgtgcggtgt atacggacac accacgatga
      361 gacctcgaaa gggttgcgca aaaagagatt gattcagcat atcgagccgt ttttccgaga
      421 aacgatgacg aaaatcagcg ttcgtcaatg gtggagcgtt caagcgacgc aacgccttgc
      481 cgagccagta gtcatcgacc cagccgcgcg cccctttggc tggccctttc aaggcctccc
      541 cttgcccatg catcgcgatc agcaccggta aacggtgatg cggacttgcc tctcggggaa
      601 ccacaacgac agcttgcatg ggtcccacgg gggtgtcgtc gaatcgtaac gtgaattcac
      661 gaacctttcc cgatgccctg ccttctttgt ttcgaatctc ttcgctcacg gaagaggatg
      721 acgcggcaga cgcaagagaa atcgctggtt ttgcttgctc cttcgaggta acggaagcca
      781 cggttttttg cgtttgcacg tccgacgggg gatgtttttc gcatcccagc atggtgcaaa
      841 ccatggccac aaaaacaatc acaggtctca cgcttgcacc gctaccatga atcatcgctc
      901 tggtcacagg gcttgcgacc cttaatgtcg aagctgtaca agggtctagc cgaggagacg
      961 atgaatccac aacaggaacg aatcgcgcaa gcattgcatg tacaatccgt aatcgacgcc
     1021 aaggtcacga tatcggagat cgtgcgtttt ctaagcagct acctgattcg ttcgggagcg
     1081 agagggtacg tgcttggaat cagcggtggt caggattcga cactatgcgg caagctggcc
     1141 caattggccg ttgaggatgc gcgtcaccac tcggcgctcc cgatatcatt ctgggcgatg
     1201 caccttcctt acggtaagca gcgagatgaa caggatgtga agcttgcgct ttcgttcatc
     1261 gagccggatc gcttggtaac cgtaaatatg caagccagtg tggatgcctc ggtcagagcg
     1321 ttggaagatg ggttgggcga gccgttgtcg gactatcacc gtggcaatgt caaagcccgg
     1381 gagcgaatgg tgatgcaata cgcattggcc ggaagccata acctgctggt gttgggaacg
     1441 gagcatgccg cggaagccgt tacaggtttt tttaccaagt acggtgatgg tgcatgcgat
     1501 ctggcgccgc tggtcggttt gacgaaacga caagggcgcc tcttgctgca atctatgggg
     1561 gcccccgcgc ggttatacga aaaagtgccg accgcggacc tggaggattt gaggccgctc
     1621 taccccgacg aggcatcctt gggaatgagc tatgaacaaa tcgatgacta tttggaaggt
     1681 aagtccgtcg atgcacaaat cgccaagcgc ctcgaagcga tctatgtcgc aaccaagcac
     1741 aaacgagcga tgcccgtcac ccgttttgga tgaggctcac caggggctcg tcgtatcgag
     1801 ttctttgatc cgatcccgcg cgcttttggg gtcttctttt ttttcggcag gcggcttggc
     1861 ttcagggggc gatgcaggcg gctgctcttg aaccaccggc tcaggtgcct tgggatccag
     1921 aacgcgggga acaacacctc cggtgcgaac cggctccaac tcgaccacga aactcgcatc
     1981 ccgtagggca gccacttcga ccgtacgagg gacaaatcct ggcgcatgtg cgtgaattga
     2041 atagactttt ccatcctgca ccacgtgcag agtcgacgga ttgtctttca tcggttttcc
     2101 atcgatggag atggtggcgt tgcttgggat ggctttgaca gcgatggtgc agcttgccgc
     2161 cggaaccatt ggtttggtcg cttcgaggga aagcgctggc ggtggaggat tcgacggttc
     2221 ggaaggcgcg ctcatctgct cggcagaagc tacggtttcg ggcggcgttc gcagcagaag
     2281 cgccgtagct cccgcaagaa tcgccagtcc gacgacagcc atgatgaccg ccgctccttt
     2341 ccgtttctca gggcgtcgag aaagccgcat cccagacaag gaagcgtcgg agttgacgga
     2401 acgaatcgaa ctttgggaat cctcgctgtg ggggtgcgaa agggttcccg tctgtgttcg
     2461 gtatccagat tcactgagtg atttcagttc ggtttcaagt gtgcgactga gcatttcacg
     2521 ctcgtctttg aacaggttcg ccacgaaagc tcccacatcg cgtgtggaaa ccgacgatct
     2581 tgcatcgatc gccgcctcga tggcgtcgtg catctccagc gcggactgga accgtttgga
     2641 gggatccact tccatcgcct tgaggcagat ggcttccaga tccgcatcca cgttttcaca
     2701 aacatcactc ggtttgggaa catcgccgtt caacaccgtg agcaagattt ggccttgcgc
     2761 ttttcccttc caccattgtt gtccggtcac caactgccaa agaacgactc cggtcgagta
     2821 gatatccgca cgatggttga tagactcgcc agacacttgc tccggcgcca tgaaagccgc
     2881 tttgcctttc atcacgcctg cccgcgtttc cgaagacgaa atcgccgctt tggcgattcc
     2941 aaaatccaac actttgattt gcccatcgaa ggtcacgaaa atgttttgtg gagacacatc
     3001 tcgatgaacc aaacgcaagg gtgttccatc gaaattcttc agagtatgcg cgtaatgcag
     3061 tccttgaagc gcctccgcaa gcacccgtaa ccccatggcc atgggcatcg gcttttcatc
     3121 ccgctggcac cttcgcaaaa tggaactgag cggctgaccg tcgagatgct ccatcacgag
     3181 gatgggacga cccgcatctt cactcacctc gagcgtatgc acgacgttcg ggtgattgag
     3241 tcttccggcc aggcgagctt catcgacaaa catggagcga gcgtccgggt cttccgccac
     3301 ctccgcacga atgattttga gaacgaccaa cttattgaaa ccgctaagtc cttgtgcgac
     3361 cgccaagaag acttcggcca ttcccccacg tccaacctcg cccaggatac ggtattttcc
     3421 gagttggaga tttcccgggt caaggcgcat gcgtacagga cggagcggag gttcgcgggt
     3481 gagatcgtct ttcgaatcag actgagaaac cattgcgatt ccgtttagaa caacaacacg
     3541 acacgttcgc gcaaagcgga gccgtgacca tccaagcaga tatattgggg tagcataccg
     3601 tgactgcacg aacaacgaag gcaatgcaaa atcattgtat ttttccttat tcacgccgag
     3661 gtcccaagaa ccgtgacaac ggattcatag ggacgacgat ggcatgattt cgacgtcgat
     3721 gcctaccctg gcctgttgaa gtcatgatgc acgacagcac gtacaaggcc ccctcggtgg
     3781 atcatctggg ttttctcgat catccggaag actggacgga agattttgca cgccaaacgg
     3841 caccaatcgt tggaatcacg caaggattga cagaccgtca ctgggctgtc attcgctacc
     3901 ttcgcagcca attcgagaag accggacgat gcccattgct ctacttcgtt tgctccgaca
     3961 acgggttgtc cgtcgccgat ttgcaggccc tatttccaag tggctacttg cgcggtgcgt
     4021 gccgcctggc tggtatttcc tatcgcgatt cgattctctg tagtccgtgc catggaaaag
     4081 acgatcgaca atgcacggcc aaagggatcg ataaagtgta tcgtgtcgat gtgctgggtt
     4141 ttctagtgga tcccgacgaa tgggacagtg ccttcgctat ccatcgcgct cgggaaatgg
     4201 gaatcgaggc agggttgacc caacaacatt gggaagtgct cgatcacctt cgaaacgttt
     4261 acgacgagac tggggacgtt ccgacggtat actccgtatg cgaggagttg gggctctcgt
     4321 tggaaacgtt ggcgcagtta tttccaagtg gatatcacag aggagcggtg aaactggcgg
     4381 gattgcgtgt tcattgacca ggctgccttt cgcacatcga tttttttgct ctcttcgaga
     4441 tggaaaatcg aatcggcgcg ttgcatggtt ccgccatgtt ttcccaggcc tctacccgaa
     4501 tcctcgttac cgtcgttttg ctgatcaccg gctgtgagcg gggcaaaacg acagatcccg
     4561 agccaactgc cacatcctct tcacccgccg tagccgcctc ctcgactcct attcccgttg
     4621 aaacgcctaa gaaaccactg aatgtcatct tgttggtggt cgatgccatg cgttacgaca
     4681 tgccctggga gggttacgac cgagctatcg ccccgaatct gacgcaactt catgccaagt
     4741 ccattgccta cgaacggggt tatgcgattt catcatttac gtccaagagc gtgggtggtt
     4801 tattgactgg acgatatccg tcatccttgg ctcggacctc tccgtttttc acatcttata
     4861 aagatgaaaa cgaattcatg gcggagttac ttcagaaacg tggaatccga acgctcggcg
     4921 tccaggcaca tatgtatttg aaatccgttg caggatttca ccaaggattc gatgtatgga
     4981 aaatggttcc gggtattcaa tgggattaca acaaagaccc atatattacc gcaccggatc
     5041 ataccaaact catcaatgag attcatgcgg aaagagaaaa tacttcaggg caattttttg
     5101 cgtattacca ttatatggat ccgcacgatg tttacaatac gcactccata gctcctgact
     5161 tcgggaaaaa agggcgtgac cgttatgatc aggagatatg gttcaccgat catcacattc
     5221 aagccatgtt agattatgtc aatacacagc cgtggggcaa gcatacggtc gttatcgtaa
     5281 ctggtgatca cggtgaggct tttggtgaac acaatttctg gaagcacgcc tttgagttgt
     5341 acgaggtgtt gatccgtgtg cctctattta tatatgttcc gggtctcgag ggtaggaaaa
     5401 tttcccgatg gcgaagtcat atcgatctcg taccgactat tttggatatg atgggtatac
     5461 ccattcccaa agacctgccg ggtgtcagct tatggccaga aattcaaggg aaggaaacac
     5521 ctgctcgacc gatcatcgct gaccttcccg ctgataccta taacgttcga aaacgggttt
     5581 tcatcgatga aaatggttat aagctctcg
//
LOCUS       DAOSOR010000395         5590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14646_length_5590_cov_4.210117, whole genome shotgun sequence.
ACCESSION   DAOSOR010000395 DAOSOR010000000
VERSION     DAOSOR010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14646_length_5590_cov_4.210117"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1779
                     /locus_tag="PK710_18040"
     CDS             <1..1779
                     /locus_tag="PK710_18040"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HOT11684.1"
                     /translation="REYRAEQETLLRKMALFSRVAELQEHRARLQDGEPCPLCGAAEH
                     PYAKGNVPVPDETEKKLESLRLLIEKAEEQEALVRKLEEAETRARDKLAEAEKLEILA
                     ANDKTTAENALKTVVDALAEWQGDYARRKQTVFDTLLGLGIEPAQDADMALVLEPLRV
                     RLASWQTHSKKKGEAEKQLAVIDTEMSRLRALLESQNAVLADKKERLEQVRQTIAVES
                     EKRKALYGDKKPEEEEARLYEAVTAREMEETRAREQHQEAHERLIATQTQIESLRTSR
                     EKRAPVLQRLQEEYSQAVETAGFSSEEQYLQALLSHEQREQLAFRAKQLDEREVELKT
                     RKAERHASLDKELVRSLTDQSLEALLPIYKKGEEELEAVRGDIAEIKIKLHDQEVAKE
                     RIKEKQQAIEAQGKECGRWDALSKLIGSADGKKFRNFAQGLTLEVMLHHANHQLRTMT
                     DRYLLKRDDATSLQFNVVDDYQGGEIRSTKNLSGGESFLVSLALALGLSQMVSQNVRV
                     DSLFLDEGFGSLDEDALDSALTTLSGLQHEGKLIGVISHVPALKARVGVQIEVTPKTG
                     GRSSISGPGCRRLDNELTPRKPGKRS"
     gene            complement(1945..2943)
                     /locus_tag="PK710_18045"
     CDS             complement(1945..2943)
                     /locus_tag="PK710_18045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11685.1"
                     /translation="MRQQHYRFSHEVMVKHVLDDPMKWMAQAQTYGPQMIHNAWTMAG
                     EGLAPQDRVDGSVPTLEPITRGPQLRGYLVTLPAPQATGECYFLAVAAAGNNQPRYFA
                     AERGNDGGDGTPRAFLAEWKASATGRMRVHNSDLPQISQAAFIEAVAASAMAPPVEAA
                     PKKKKANVPLLIGCGCAVPLAFGIIVGSCLFYYESILETYDPTSEVVSVPIHPNQPAK
                     FEFKWDGHGYAFNNFWLVVENGTKENKEFTFSGNISCTPSPSYKTFETSLSYGKTYHV
                     KEIDKDGFSAWIYLNDHYAKSASEPMKCTATIKPSKGTWTTARIVVTQRQRPSDWL"
     gene            complement(3191..5278)
                     /locus_tag="PK710_18050"
     CDS             complement(3191..5278)
                     /locus_tag="PK710_18050"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOT11686.1"
                     /translation="MVLYRRIETDLTHRCNPKDVQEFVASPPPRFRGEHVSPKDTEMN
                     YFGISLPARAFAYTGITGLLCLAAATLTNCSPDQEPAKGQLMLVVQTDMPIPKDVDSI
                     RIEISAYGNLLFGNNYAVGPSRLLIPATLAIVAGEQETLPIHIRLTSFQESRPRTLRE
                     AVTTVPKDRIGTLRLPIQWLCDGQVKMVNGQVESTCPLGQTCVAGQCMDNKVDSSHLE
                     AYSPRTVFGGGSGAGDGKCFDALPCFSEGAGAEVDRATCSIAEPVTGIGTNVALVMPP
                     KSAGICGPDACLIPLDAHSATGWQSEAGRIVLPSAVCDRLENKQIVAVAVTTACETKT
                     ESIPLCGPWSSVIEKPGDFDAKGPDVEQLREDASTQDTTEPPDAEPDTDGGTTPHEVP
                     GPSCKDMTGKECEGKDCCSTILLPGSSFPMGRSQNGNDVCPSNESCEADEQPEHTVKL
                     SEYYLDEFEVTVGRFRRFVDQFDGTPPAKGDGAHPAIPGSGWQSGWNSNLPASKAALL
                     TELNCDSLYQTWRDTPNGTEQLPINCVSWPVAFAFCAWDGGRLPTEAEWENAAAGGDE
                     NRLYPWGVTPPDPTLASCYCLYGKNTNCTFSDIAPTGALPKGQGRWGHKDLAGNMMEW
                     VLDWWADNWYAEGGNTCVDCANILLGEYRVLRGGSWVQTQATYFRNASRAYDHPDTRL
                     SSRGFRCARDKNP"
BASE COUNT         1274 a         1417 c         1631 g         1268 t
ORIGIN      
        1 agggaatacc gggcggaaca ggaaacgttg cttcgtaaaa tggccctttt ttcgcgtgtt
       61 gcggagctgc aagagcatcg cgcccggctg caagatggcg agccgtgtcc tctttgtggg
      121 gcggcggagc atccgtatgc gaaaggcaat gttcccgtcc cggatgaaac cgaaaagaag
      181 ctcgaatccc tcaggctctt gatagaaaaa gcggaagagc aagaagcgct ggttcggaag
      241 ctcgaagagg cggaaacgcg ggcgcgcgac aagctggccg aagcagaaaa gctagagata
      301 cttgccgcca acgataaaac gactgcggaa aatgctttga agacggttgt cgatgccttg
      361 gcagagtggc aaggggatta tgctaggcga aagcagacgg tgttcgatac gcttttgggt
      421 ctgggaatcg agcccgccca agacgcagat atggctttgg ttctcgagcc ccttcgcgtc
      481 cggctcgcgt cctggcaaac ccatagtaag aagaaagggg aagcggaaaa acagcttgcg
      541 gtcattgata cggagatgag ccgattgcga gccctgctcg agtcccagaa tgccgtgttg
      601 gcagacaaaa aagagcgttt ggaacaggtg cgccaaacta tcgccgtgga gagcgagaaa
      661 cgcaaggcat tgtatgggga taaaaaaccg gaagaagagg aagctcgctt gtatgaggca
      721 gtgactgcgc gggagatgga ggaaacgcgg gcaagagaac aacatcagga agcccatgaa
      781 aggttgattg cgactcagac gcaaatcgaa tcgctgcgta caagccggga gaagcgagca
      841 cccgtgctcc aacggttgca ggaagagtac tctcaagccg tcgaaacagc cggattttcg
      901 agtgaagagc aatatttaca agcgcttctc tcccacgagc aaagggagca attggcgttt
      961 cgggccaaac aactggacga gcgtgaggtg gagttgaaaa cccgaaaggc ggaacggcac
     1021 gcttctcttg acaaggaatt ggtgcgttcg ttgacggatc aatccttgga ggcgctcttg
     1081 ccgatttaca aaaaggggga ggaggagctt gaggccgtac gtggagatat cgctgaaatc
     1141 aagattaaac tacatgatca ggaagtggcg aaagagagaa tcaaagagaa acagcaagcg
     1201 atcgaagcgc aaggaaagga atgtggccgc tgggacgcgt tgtcgaagct gatcggttcc
     1261 gcggatggca aaaaattccg caattttgcc caaggattga ctctcgaggt gatgttgcac
     1321 cacgccaacc accaattgcg gaccatgacg gaccggtatt tgctcaagcg agatgacgcc
     1381 acgtcattac agttcaatgt ggtcgatgac taccaggggg gggagattcg ttccacgaag
     1441 aacctctcag ggggggaatc cttcctggtc agtttggcgc tggcgttggg cttgtcacag
     1501 atggtgagcc aaaacgttcg ggtggactcg ttgttcttgg atgaaggatt tggttctttg
     1561 gatgaggacg cgctggattc ggcactgaca accctttctg gcttacagca tgagggcaag
     1621 ctgattggcg tgatttccca tgtgccagcc ttgaaagcgc gagtgggagt tcaaatcgaa
     1681 gtcacaccga aaacgggagg caggagttca atctccggac cgggatgtcg tcgcttggac
     1741 aatgagctga ccccccgaaa accgggcaag agaagctgag atccgatacg ttccaatagt
     1801 catagcttac tgatatcatt ggaaaataat tggatccggc agaagcacgc agcatcatgg
     1861 aggatcgagg aaacgaccgc tgtctgcgga tactcggggg cagatagggc ttgcgttctt
     1921 gaaggttttg cgaataattg gcgtttatag ccaatcacta ggacgctgtc tttgggttac
     1981 cacgatccgt gctgtcgtcc atgtcccttt ggagggtttg atggtagcgg tgcatttcat
     2041 tggctctgac gcgcttttgg catagtgatc gttgaggtat atccaggcag aaaagccatc
     2101 tttatcgatc tccttgacgt ggtacgtctt gccataggaa aggctcgtct cgaaggtttt
     2161 gtaggatggc gaaggcgtgc aggaaatatt gcctgaaaac gtgaattcct tattttcttt
     2221 cgtgccattt tccaccacca accagaagtt gttgaaagcg tagccatggc cgtcccactt
     2281 gaactcgaat tttgcgggtt ggttcgggtg aatcgggacg ctcaccactt cgctggttgg
     2341 atcgtaggtt tcgaggatcg actcataata aaacaggcaa ctgccgacaa tgatgccgaa
     2401 tgccaggggc acggcgcaac cgcagccgat gagcaaaggg acgttggcct tctttttttt
     2461 tggcgcggcc tcgacagggg gagccatggc ggaagccgcg acggcttcga tgaacgcagc
     2521 ctgggaaatt tgcggaaggt cgctgttgtg aacgcgcatc cgcccggtgg ccgacgcttt
     2581 ccactcggcc aagaaggcac gaggggtgcc atcgccccca tcgttcccgc gctctgctgc
     2641 gaaataccga ggttggttgt tgcccgccgc ggcgaccgcc aggaagtagc attcccccgt
     2701 ggcctggggt gctggtaagg taaccagata acctcgaagt tgaggtcccc gggtgatggg
     2761 ttcgagggtt ggaacgcttc cgtccacgcg gtcttgaggg gcaagacctt ctccagccat
     2821 ggtccaagca ttgtggatca tctgcgggcc ataggtttgc gcctgtgcca tccacttcat
     2881 gggatcgtcc aagacgtgct tgaccatcac ttcgtgggaa aaacggtagt gctgttgtct
     2941 catggaacct cgataaatag gtgaactgtg caaaaagtct cacggtttta cgcacctgtc
     3001 aagagccctg ggggtcgaag caagagaatt tcgtgtcttg cctggatttg gatgtatgga
     3061 tccggttttg gtcgttattg ccaattcgcc tagctattgt ctattcaagc gaatgagcgt
     3121 aggcccatgg tgagatagta ttagtaaccc ttcctaaaca tgggaaatca aatcaccaac
     3181 atgatttccc tcaagggttc ttgtcccggg cacagcgaaa tcctctcgag cttagtcgag
     3241 tgtcagggtg gtcgtaggca cgactggcgt tgcggaagta cgtggcttgc gtttggaccc
     3301 agctacctcc ccgcaacaca cgatattccc ccagcaagat attggcgcaa tccacgcagg
     3361 tattacctcc ttcggcatac caattatcgg cccaccaatc gagcacccac tccatcatat
     3421 tgcccgcgag atccttatgt ccccatcgac cctgtccttt tggcaatgcg cccgtcggcg
     3481 cgatatcgct aaaagtgcaa ttcgtgtttt ttccgtagag acaatagcag gaagcgagag
     3541 ttggatccgg tggggtaaca ccccaaggat acaagcgatt ctcgtcgccc cccgccgcgg
     3601 cgttttccca ctccgcttcc gtgggaagcc tccccccatc ccacgcgcaa aatgcaaagg
     3661 cgacgggcca gctcacgcaa ttgatgggaa gttgctccgt gccgttgggc gtgtcgcgcc
     3721 aggtttgata cagcgaatcg caattgagtt cggtcagcaa tgccgctttg gaggccggca
     3781 agttgctgtt ccaaccgctt tgccaaccac ttccgggaat cgccggatgc gcgccatctc
     3841 ccttcgccgg tggtgttccg tcgaattgat cgacgaaccg gcgaaatcga cccaccgtta
     3901 cctcgaattc gtccagataa tactcagata acttcacggt atgttcaggt tgttcgtctg
     3961 cctcgcaaga ctcgttgctc gggcatacgt cgtttccgtt ttggcttcta cccatcggaa
     4021 acgagctgcc tgggaggagg atggtcgagc agcaatcttt tccctcgcat tcctttcctg
     4081 tcatgtcctt acaggatggg cctggaactt cgtgaggcgt cgttccgccg tctgtatccg
     4141 gttcggcatc cggtggttcc gttgtgtcct gtgtcgatgc atcttcccgt aattgctcga
     4201 catcgggccc tttggcgtcg aaatccccag gtttttcaat gactgaggac cagggaccac
     4261 aaaggggaat gctttctgtt ttcgtttcgc acgccgtggt caccgccacc gctacaattt
     4321 gcttgttttc aagcctgtcg cacaccgccg acggcaggac gatccgtcca gcctcggatt
     4381 gccatccagt ggcgctatga gcgtcgaggg ggatcaaaca cgcatccgga ccgcaaattc
     4441 cggctgattt cggtggcatg accagggcaa cgttcgtgcc tatacccgta acgggctcgg
     4501 caatcgagca tgtcgctcga tccacctccg ctcccgctcc ttccgaaaag cacggcaaag
     4561 catcaaaaca ctttccgtcg ccggcaccgc ttcccccgcc gaagaccgtc cgtggcgaat
     4621 aggcctccag gtgggaggag tcgaccttat tatccatgca ttggcctgca acgcaggttt
     4681 gccccaaagg gcaggtgctc tccacttgcc cattgaccat cttgacttga ccgtcgcaaa
     4741 gccactgaat cggaagacgt aaggttccaa ttcggtcctt ggggacggtg gtgaccgcct
     4801 cccgaagcgt acgaggtcgt gactcttgaa acgatgtcag ccggatatga attggtagtg
     4861 tttcttgttc gcccgccacg atcgcgagcg tagcggggat gagcaacctg gagggaccta
     4921 ccgcgtagtt gttgccaaac aggaggttgc cgtacgcact gatctcgatc cgaatcgagt
     4981 ccacgtcttt ggggatgggc atatccgttt gcaccacgag catgagctgg ccttttgcgg
     5041 gctcctgatc gggagaacaa ttcgtcagcg tagccgcggc aaggcataac aacccggtaa
     5101 ttccagtgta ggcgaaggct cgagcaggga gactgatgcc gaagtaattc atttcagtat
     5161 cctttggcga tacgtgctcg ccacggaagc ggggaggggg tgaggctacg aattcttgca
     5221 cgtctttggg attgcatcga tgggtaagat cggtttcgat acgcctatat aataccaaga
     5281 cgcaaccgtg aacaggtggc ttgatgcacg ttcttcgtgc gacgatagaa atcggctacc
     5341 atcatttttt ttcttggatt cggtgataaa aatctggact gtaaagacaa actacagggg
     5401 tgcctttggc tttcgttccc gcgacgtgat accaggggca tgatgggtcg ggtcgcttgt
     5461 ggggctggtt tccgatgggc ccattgccag gacttatgag ctaacgactc gttttcaagg
     5521 gaataaagtg ttgtaaccta ttgatattca aatttgtttt tggagtacgt cgtggtctgg
     5581 gggcgctata
//
LOCUS       DAOSOR010000396         5582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14678_length_5582_cov_4.780713, whole genome shotgun sequence.
ACCESSION   DAOSOR010000396 DAOSOR010000000
VERSION     DAOSOR010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14678_length_5582_cov_4.780713"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PK710_18055"
     CDS             <1..274
                     /locus_tag="PK710_18055"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOT11687.1"
                     /translation="VAHHAPFDRAVLRACCQRARLPMPSLPFKCTVKWSRRMFDLPRY
                     NLPTVCDYLGIALDHHQALSDAEACARIMIEVQRRWQQRDVSTAAR"
     gene            complement(276..926)
                     /locus_tag="PK710_18060"
     CDS             complement(276..926)
                     /locus_tag="PK710_18060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11688.1"
                     /translation="MNVVTHGGSEGLSTAQRCVRDLAKAWTPCQKGMVPPSEETPVEG
                     RMFEQKAISIPKDWGLQAFADAVEAILGELATERWESRIFQLGPQGLLIVARRPRFVD
                     IDDLMMLDSPDWQQGTDVEEQDDLAPATREMLDKIFDLVGPVSLHAALEGMPSAVHGV
                     ARLYPPRFLEEAWSELQRHHEQHLREHNEGEEACEYAQVVAVVLESMREGLRLRLN"
     gene            972..1664
                     /gene="aat"
                     /locus_tag="PK710_18065"
     CDS             972..1664
                     /gene="aat"
                     /locus_tag="PK710_18065"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOT11689.1"
                     /translation="MFGPVRLTDDLIFPPASWARRDGLIAVGGDLRPERLILAYRSGI
                     FPWPFDEDSPLLWFSPGIRMVLPIEALHVSHSLRKVLRRKTFRVTLDEAFSDVIHHCA
                     HVSRGPDTGTWITPAMIEAYTILYRLGLAHSVEVWHQDQIVGGLYGVSLGAGFFGESM
                     FSLLSNASKVGFVTLVQFLAQRNFHFVDCQLYTNHLANFGAQRWRRTRFLKALEHALS
                     FPTLQGPWTIDP"
     gene            1823..3118
                     /locus_tag="PK710_18070"
     CDS             1823..3118
                     /locus_tag="PK710_18070"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HOT11690.1"
                     /translation="MRPVRLITTALLTVCLSSCSSDQTTDEPVIPKGAIEQRDGFTIV
                     WLHGTPYEMGYQHGTLLREQLREAAQVVLNEGLFKILKNAAKIAKLDEVALANSYPWL
                     LEECKGMVKAVDDPDFQMFECVLINFGDVAVEFLNYGMPQFEDIAPGCSQVVATGSAT
                     SDGSLYHARVLDWYAVDFVVNNPVIFVRQPKDGIPHLFVGFPGNLSSYQGMNAEGIVV
                     ASNEVNPRDTTVNDLTGRSHVQMVVEILTHASSLDEARTIVKTSNHMSLETLVVSDGK
                     QKTAEVFELAPAQVGVRPLKDDVVYATNHFVESNTADLDAEKPNKSTYLRWDRLTQLV
                     TKDGAESKWGKLNPETLIAVMRDRVNPETGQPVPDDIVDNGESLATNGALYQVVFAPE
                     TLSLWVAAGKIPVPSQPFVGFNLGELLHREGYSTPATLP"
     gene            3454..4530
                     /locus_tag="PK710_18075"
     CDS             3454..4530
                     /locus_tag="PK710_18075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11691.1"
                     /translation="MHTPPHLPSKHELSPSLLRAHSLALSRGLRDTVEMQSASARFLS
                     ELARQATVPEARKLLLELARIEQLLSLEIDVKVAQLVEAPLPARASPGAANIKTAPEW
                     AHLELITYEQAIAVTLDCTRRSILFLTDMANRFDGPTLDFFLMLAQSDEDRAAMLERA
                     LNRRLAELTIGHDIRRVLVEIHDAARRAAKAYAWIGRRTMRPRAQEFLQGMQEVCALH
                     AANIQSMLGNMDHLSDDPVFEGLAAATLPTLDGDVEGLDFQAALRIAMHAQQRTALVH
                     GMYARAFRGEEAQQLLDIADAERRQAATIASVLERLEPSDQDMEEQYPQARSPRESWT
                     CPAIVDESEALDEPKTPVLRLVAG"
     gene            4717..5196
                     /locus_tag="PK710_18080"
     CDS             4717..5196
                     /locus_tag="PK710_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11692.1"
                     /translation="MSIRGCGRLSFFVAMMGSLSLVGAECGGGDDNAAGTGNASSDLA
                     GTWCLIKTLVTATEDHPAGKQFPLELVFVVHGNSATMSTPGVSGSAEGTLSEGVWTFT
                     YEIPIAPGLTSLNQVQVISTSPFKGTEEHRYYDQFGQQIGLEASTLEGWRKTTESCW"
     gene            complement(5258..>5582)
                     /locus_tag="PK710_18085"
     CDS             complement(5258..>5582)
                     /locus_tag="PK710_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11693.1"
                     /translation="AGAARGAGAALGAGGGVAQEDGTRMAAVPPPEETGTAVVAESMA
                     AAPPPEEIGTEAPGDMPVIRGMRAGLRPVVEAPSMAAPLALVAPDNPLIPASRILPFL
                     TFFNS"
BASE COUNT         1179 a         1756 c         1480 g         1167 t
ORIGIN      
        1 cgtagcccat catgcgccgt tcgaccgcgc ggtgctgcgt gcttgttgtc aacgcgctcg
       61 cttgcccatg ccttctttgc ctttcaagtg tacggtgaaa tggtcgcgac gcatgttcga
      121 tctgcctcgc tacaacttac ccaccgtctg cgactatctt ggcattgctc tcgatcatca
      181 ccaggcgctg tcggatgccg aagcctgtgc ccgcatcatg atcgaagtgc agcgacgatg
      241 gcagcagcgc gatgtgtcga cagcggcgcg ctgattcaat tcaagcgcag acgaagacct
      301 tcgcgcatcg attccaatac caccgccacg acttgcgcat actcacaagc ttcctcgccc
      361 tcgttatgct cccgaaggtg ttgctcgtga tgacgttgaa gttcggacca agcttcttca
      421 agaaatcgcg gtgggtacag acgcgccaca ccatgcaccg cgctgggcat gccttccaac
      481 gccgcatgca acgaaacagg ccctaccaag tcgaatatct tgtcgagcat ctcacgcgtg
      541 gcaggcgcca aatcgtcttg ctcctcgacg tccgtacctt gctgccaatc cggtgagtcg
      601 agcatcatca agtcgtcgat gtcgacgaaa cgtgggcgac gcgccacgat caacaatccc
      661 tggggtccga gttggaaaat gcgactctcc caacgctcgg tcgcaagctc gccgaggatt
      721 gcctccacgg catccgcgaa ggcttgcaat ccccaatcct tgggaatgga tatggctttc
      781 tgctcgaaca ttcgcccctc gacgggtgtt tcctcactcg gcggcaccat gcccttctga
      841 cacggcgtcc acgctttggc aagatcccga acacaccttt gtgccgtcga caatccctcg
      901 gacccaccgt gcgtcacaac gttcaaaccg catcgattga cgccttagga gttcaagaac
      961 agaatccgat catgtttggc ccagttcgtc tgaccgacga cctgattttc ccgcctgctt
     1021 cctgggcacg acgcgacggc ctgatagccg tcggtggcga cctgcgaccc gaacggctca
     1081 ttctcgctta tcgaagcggg attttcccct ggcccttcga tgaggactcg cctttgctct
     1141 ggttctcccc cggcatacgc atggtgttgc ccatcgaagc gctgcatgtc tcccatagtc
     1201 tgcgcaaggt gctgcgcaga aaaacgtttc gcgtgacctt ggatgaggcc ttttcggacg
     1261 tcatccacca ttgtgctcac gtatcgcgcg gccccgacac tggaacttgg attacgcccg
     1321 cgatgatcga agcctacacg atactctacc gcctcggtct cgcccatagc gtggaggtct
     1381 ggcatcagga ccagatcgtg ggtggattgt acggcgtgtc tctgggcgcg ggcttcttcg
     1441 gcgaatcgat gttctcgctg ctatccaacg cctccaaagt tggcttcgtc accctcgtcc
     1501 aatttctggc tcagcgtaac ttccacttcg tcgattgcca gctttacacc aatcacctcg
     1561 ccaattttgg tgcccagaga tggcgacgca cacgttttct caaagccctc gaacacgcct
     1621 tatccttccc cacgctgcaa ggaccgtgga ccatcgatcc atgagaacct tgacctttca
     1681 tcccatccgc aaagcaacga cccttcccat acgccaaccc gcccgcccct accgccatga
     1741 caacctctca gccattcgag cccatatcgc gaaatggagc gattcactgg cgcctttccc
     1801 acggctcgct tacattcgaa ccatgcgccc cgttcgtctc atcaccaccg ccctgcttac
     1861 cgtctgcctt tcatcgtgct cgagcgacca aaccaccgac gaacccgtga tccccaaggg
     1921 agctatcgag cagcgcgacg gcttcaccat cgtttggttg catggaacac cgtacgagat
     1981 gggctaccag cacggaacgt tattgcgtga gcaactgcgg gaagccgctc aagtcgtgct
     2041 gaacgaaggc cttttcaaga tcctaaaaaa tgcagcgaaa atcgcaaaac tagacgaagt
     2101 cgccctcgcc aattcctatc cctggctcct cgaagagtgc aaaggcatgg tcaaagccgt
     2161 cgatgacccg gacttccaaa tgttcgaatg cgtcctcatc aacttcggtg atgtggccgt
     2221 cgaatttctc aactatggca tgccgcaatt cgaagatatc gcccccggct gctcccaggt
     2281 cgtcgccacc ggaagcgcca cttccgacgg ttccctgtat cacgcacgag tcctggattg
     2341 gtatgccgtc gatttcgtgg tcaacaaccc cgtgatcttc gttcgtcagc ccaaagatgg
     2401 catccctcat cttttcgtgg gttttcccgg caacctgtcc agttaccaag gaatgaatgc
     2461 ggaaggaatc gtcgtggcct ccaacgaggt caatccccgg gacactaccg tcaacgacct
     2521 gacaggtcga agtcacgtgc agatggtggt cgagatcctc acccacgcct ccagcctcga
     2581 cgaagcacga accatcgtca agacatccaa tcatatgagc ctggagacgc tagtcgtttc
     2641 cgatggcaaa cagaaaacgg ccgaagtctt cgaattggcg cccgcgcaag tgggtgtccg
     2701 gccgctgaaa gacgatgtcg tatacgcgac caaccatttc gtggaaagca atactgcgga
     2761 tctcgacgcg gaaaaaccta ataaaagcac gtacttgcgg tgggatcggt tgacgcaatt
     2821 ggttaccaag gacggagcgg aatccaagtg gggcaagctg aatccggaaa ccctgattgc
     2881 cgtgatgcgt gaccgtgtga atcctgaaac agggcaaccc gttcccgacg atatcgtcga
     2941 caacggtgag agtctggcta cgaatggcgc gctgtatcag gtcgtttttg cgcccgagac
     3001 cctttcgtta tgggttgccg cgggaaagat ccccgtgcct tcccagcctt tcgtggggtt
     3061 caatctgggt gaattgctcc atcgcgaagg ttattccaca ccggcgaccc taccctgagt
     3121 aagccaatcc aaagcataac ccacttgcga atacctcgat cgaaacgctg ttcacgatcc
     3181 ccgccagcga acgaacctcg ttgcaagcag tcagagtcgc gctcggaatc ccgctagaac
     3241 caccaaaata cagagccgtt gttgaatttc cagacactcc gcacaatctc tctccctggt
     3301 tgtcgacttc gagagaatta tgaactgtat gcctatatcc ctcttgcaca cctcaatcga
     3361 ccacgttaga ggcatttgtc ttacaacttc cgcaacctcg aatcctcgtc tcacatccaa
     3421 ttttgggtca ggcgacgttc aggagtaggg aagatgcata ccccaccaca tttaccatcg
     3481 aagcatgagc tgtctccaag cttgctgcga gcgcattccc tggctctatc tcgtggtcta
     3541 cgagataccg tcgaaatgca gtctgcctcc gcgcgctttc tgagcgaact cgcgcgacaa
     3601 gccaccgtgc ccgaagcaag aaagcttttg ctcgagcttg cgcgcatcga acaactgcta
     3661 tcccttgaga tcgacgtcaa ggttgctcag ctcgtggaag cgccgctgcc ggctcgcgct
     3721 agtccgggcg cagccaatat caaaacggct ccggaatggg ctcacctcga actcatcacg
     3781 tacgagcagg ccattgccgt gacgctcgat tgtacccgtc gatccatcct gtttttgacg
     3841 gatatggcga accgtttcga tgggccaacc ctcgacttct ttttgatgct tgctcaatcc
     3901 gatgaagacc gcgctgccat gcttgagcga gcgctgaacc gaaggctcgc ggagttgacc
     3961 atcggccatg atattcgccg ggttttggtg gaaatccacg atgcagcgcg tcgcgcggcc
     4021 aaagcctacg cgtggattgg acggcgtacg atgcggcctc gcgcacagga gtttttgcag
     4081 gggatgcaag aggtctgcgc gctgcatgca gccaatatac agagcatgct gggaaacatg
     4141 gaccacctat ccgatgatcc tgttttcgaa ggtctcgccg cggcaacctt gcccacgctc
     4201 gacggtgatg tcgaaggtct cgatttccaa gccgctttgc gtatcgccat gcatgcacaa
     4261 caacgaaccg cactcgttca tgggatgtat gcacgggcat tccggggaga agaagcgcaa
     4321 caattgctcg atatcgctga tgcggagcga cgccaggccg ccaccatcgc gtcggttctc
     4381 gaacgtctcg agccttcgga ccaggacatg gaagaacaat atccgcaagc gcgtagtccc
     4441 cgggaatcat ggacctgccc tgccatcgtc gacgaatcgg aagcgttgga cgaacccaaa
     4501 acgcccgtct tgcgtctggt cgccggctga tcccctcttt cctatccaag ctggcagccc
     4561 atttgccctt ttccataccc ggccatggca cgaataggcg tcgaaaattc atggtgaatc
     4621 gagcgcttgg ccccggattg gggtatcatg aacccaaggc atgaatcgtg gaatcatcct
     4681 caatactgtc gggaaagcgt gccagaggga ggcgtcatga gcatccgtgg ttgtgggagg
     4741 ctttctttct tcgtcgcgat gatgggcagc ctcagcttgg tgggggctga atgcggtgga
     4801 ggcgacgaca acgccgccgg aacgggcaat gcctcgtcgg acctggcggg aacatggtgc
     4861 ctcatcaaaa ccctggtgac agccacggag gatcatcccg cgggcaaaca atttccgctg
     4921 gaactcgtgt ttgtggtcca cggcaatagc gcgaccatgt ccacgcctgg agtcagcggg
     4981 agcgctgagg gaaccctgtc cgagggggtt tggacgttta cctacgaaat cccgatcgct
     5041 cctgggctca cgagcctcaa tcaagtgcaa gtgatcagca cgtcgccgtt caaagggacg
     5101 gaggagcatc ggtattacga ccaattcggg cagcagattg gtctggaggc atcgacgttg
     5161 gaagggtggc gaaaaacgac ggaatcctgt tggtgagggg gggctcgcgg gcagtcgcaa
     5221 gggattgtcg atcgtcaagg gcgatgcggg tcgagcttca tgagttgaaa aaggtgagga
     5281 aaggtaagat gcgagaggca gggatcaacg ggttatcagg agccaccagc gccagcggtg
     5341 ccgccatcga tggcgcttcc accaccggcc gcagccccgc ccgcatccca cggatcaccg
     5401 gcatatcccc cggtgcctcc gtcccaatct cctccggcgg cggcgctgcc gccatcgatt
     5461 ccgccactac cgccgtccca gtctcctccg gcggcggcac tgccgccatc cgggtcccat
     5521 cctcctgagc caccccaccc cccgccccca gtgccgcccc cgccccccgt gccgccccag
     5581 cc
//
LOCUS       DAOSOR010000397         5573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14711_length_5573_cov_5.453425, whole genome shotgun sequence.
ACCESSION   DAOSOR010000397 DAOSOR010000000
VERSION     DAOSOR010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14711_length_5573_cov_5.453425"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PK710_18090"
     CDS             <1..310
                     /locus_tag="PK710_18090"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HOT11694.1"
                     /translation="TAVGAAQNTTSEVVARLERVGLVRKEKDPEDQRAVLVSATERGL
                     QALRERRAALRSVHRMILQALCVEDRKRFIDASEQLVELTEKARKAMAGTSILPRNGA
                     "
     gene            400..1905
                     /locus_tag="PK710_18095"
     CDS             400..1905
                     /locus_tag="PK710_18095"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT11695.1"
                     /translation="MAQPADPTPDTASSSAEAPKATAEAPAPPDAAHVRQRLNEALAP
                     QPGGLRAADVARRAEMSSPEVRAKQQAVAAAAAKVDQAAWLFLPRATLLARYVRLSEV
                     ASANLSDRGSILGTDLPAGQPVPVVCPMGPGSTPDQCYVRAQGVELSFPILLNMWTLQ
                     GTLAIPLSDYVLRLSQNHASATNSKRAAETQEKAARVKTQLDAKTAYFNWIRAQGGLL
                     VAEAGLATAGAHLKDVRVAFQVGSASRADVLAVESQVASTEMLVENSKNMTTLTEEQL
                     RVMMHDPERTQYRIGENIDAPVADEPASTTLNALFAEAMRQRLERRVIDETVASLRET
                     RKVLSASNYPRLDAFGNLYHQNPNTRVFPQQEKWSTTWDVGVQLSWTINDTFTTGAQV
                     QELDAKILEIESQRAQLDDGIRLQVTQAYNAFRQAQVGKETTERQLVAAEESYRVRRE
                     LFRAGRATSSELTDAETALVKAGFDGLNARIDLRLARENLDHALGRDAVRP"
     gene            complement(1912..>2644)
                     /locus_tag="PK710_18100"
     CDS             complement(1912..>2644)
                     /locus_tag="PK710_18100"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT11696.1"
                     /translation="AEAELFGHAKGAFTGASRARPGLFREAHGGTILLDEISEMDMST
                     QGKLLRVLQERTVRPVGEDRDDSVDVRVLATTNKDLKAEVAAGRFREDLYYRLDVVEV
                     RLPPLEERMDDVPVLITHFLDMYQNRFATGPLTLTAALREHLQAGPFPGNVRELEHRV
                     ERAVALSTGGVADATWLMHPRSEEADKASFGLKERVDAYERGILVHELAQCRGNRSEA
                     ARRLGIARVTLLEKLKKYGITRDSE"
     assembly_gap    2645..2654
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2655..3259)
                     /locus_tag="PK710_18105"
     CDS             complement(<2655..3259)
                     /locus_tag="PK710_18105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT11697.1"
                     /translation="MKPRVLIVDDDTGVRYTLREILESAEMDVIEARDGVEALGWLQT
                     HRADLVITDLQMPRLDGLQLLERLQAAPPAPKTILLTAHGSERHAVRAMKLGAFDYFS
                     KPFDTEEVLGVVERAVASVRAEQENEQLRAELLLSRHVVFASEAMKRIALLVYRVAPK
                     DVTVLITGPSGTGKERIAGAIVAGSPRASSPFIRFNCAAIP"
     gene            complement(3256..4620)
                     /locus_tag="PK710_18110"
     CDS             complement(3256..4620)
                     /locus_tag="PK710_18110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT11698.1"
                     /translation="MTDDRTEAPHSIRYAAHELWQQEMDELVRRVFRARMLLLPLLLI
                     VALVFLLFDTTPWKVWLIFGSVIAVGVLFAFERRRLRHQRANEGTVQINLLFALGIQS
                     VLVYVTGGFTSPMLVVFVPLVALAGLGLTQRWRLVSVVTVPFVIITLLALGVVTDLLP
                     SMIPAFLHPQDSPVKGAAWLVARAAMLFVVLIITTIATHTARAAFERVVREAIQIRQH
                     ALDTLESRNRELLSTGVTIAHELKNPLSSIQGLTQLMARRMEPGTKDKERTDVMLREI
                     QRMSTVLDEFRNLTRPLSGLSRGSVALTELVQSVVTLHEGVALRRGVSLTLETNTPIV
                     VECDASKVEQALVNLVQNALEASPQGTTVRIDISESSETVSIRVRDQGPGIDKHQYNQ
                     IFTPGFTTKERGTGIGLIVARSVIEQHGGTLRLNHGPTGGTEATIVLPRHAQDTMNAG
                     EALR"
     gene            4843..>5573
                     /locus_tag="PK710_18115"
     CDS             4843..>5573
                     /locus_tag="PK710_18115"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOT11699.1"
                     /translation="MRAMVLEAPGSPLVQRLLPNPVPSAGQVLVDVSVCGICRTDLHV
                     VDGELTEPKLPVIPGHQVVGRVAGWGAGVTGFSLGDRVGIPWLGGCCDACEACIRGQE
                     NLCDASRYTGYQIDGGFAEQCVADAGFCFPIPEGYPDLQAAPLLCAGLIGYRALGMAG
                     EGHKLGLFGFGAAAHIIAQVAQYQQRQVYAFTRDGDTSSQQFARSLGAVWAGGVSDSP
                     GVMLDAALLFAPVGSLVPASLKSVR"
BASE COUNT         1150 a         1569 c         1656 g         1188 t
ORIGIN      
        1 gacggcggtg ggagcggcgc aaaacacgac gagtgaggta gtggctcgtt tggagcgtgt
       61 gggattggtt cgcaaagaaa aagaccccga ggaccagcgg gccgtgttgg tatctgcaac
      121 ggaacgaggt ttgcaagcac tgcgcgagcg gcgagccgcg ctacgctcgg ttcaccgaat
      181 gatcttacaa gctctttgcg tggaagatcg caaacgtttc atcgatgcct ccgagcaact
      241 cgtagaactc acggaaaagg cccgcaaagc catggcggga acctcaattc tgccccgaaa
      301 tggggcgtaa caacgaagga gacggtaatg gtaggacgtg gatcgagact aggggggggc
      361 ggttttgccc tgatgtgctt tgccgtttca gcgacggcca tggcgcagcc cgcggatcca
      421 acgcctgata ccgcgtcatc ttctgccgag gctcccaagg caacggcaga agcaccggcg
      481 cctccggacg cggctcacgt gcggcaacgg ctcaacgaag cgttggcgcc tcaaccggga
      541 ggtttgcgtg cggcggatgt ggcgcggcgc gccgagatgt cgagtcctga agttcgagcc
      601 aagcagcaag cggtggctgc ggcggcagcg aaagtcgatc aggcagcttg gcttttcctt
      661 cccagagcta ctctgcttgc ccgttacgtg cggttgtcag aagtggcttc cgccaacctt
      721 agcgataggg gctctatcct gggcaccgac cttcctgccg gacaacctgt tcctgttgta
      781 tgtcccatgg ggccaggaag cacaccggac cagtgttatg tgcgtgcgca aggggtggag
      841 ctttctttcc ccattcttct gaatatgtgg accctgcaag gaacattagc cattccgttg
      901 tctgattatg tgttgcgctt gtcgcagaac cacgcttcgg cgaccaactc caagcgcgct
      961 gcggaaacgc aggaaaaagc cgctcgagtc aaaacccaac tcgatgctaa gaccgcgtat
     1021 ttcaattgga ttcgagcaca aggggggctg ctcgtggcgg aggcgggcct ggctaccgct
     1081 ggcgcccacc tcaaagacgt tcgcgtcgca tttcaagtgg gaagcgcctc acgcgccgat
     1141 gtcctcgctg tcgaaagcca ggtggcttcc actgaaatgc tcgtcgaaaa cagcaaaaac
     1201 atgacgaccc tcaccgaaga gcaacttcgt gtcatgatgc acgaccccga aagaacccag
     1261 tatcgtatcg gggaaaatat cgatgcgccc gtggccgatg aacccgcatc gaccacgctc
     1321 aacgcgcttt ttgccgaggc gatgcgccaa cgcctcgaac gtcgtgtgat cgatgaaacg
     1381 gtggcgtctc ttcgcgaaac ccgaaaagtc ctaagcgcca gcaactatcc gcggctcgat
     1441 gccttcggca atttgtatca ccaaaacccc aatacccgcg tgtttccgca gcaagagaag
     1501 tggtcgacca cgtgggatgt cggtgtgcag ctttcctgga cgatcaacga tacgttcacc
     1561 accggcgcgc aagtgcaaga actcgatgca aaaatcttgg agatcgagtc gcagcgtgcc
     1621 cagctcgatg acgggattcg cttgcaggta acccaggcct acaacgcatt tcgtcaagcg
     1681 caggtgggca aggaaaccac cgagcgacag cttgtggcgg cggaggaaag ctaccgcgtg
     1741 cgacgagagt tgtttcgggc gggccgagcg acgagcagtg aattgacgga tgcggaaaca
     1801 gcgctggtca aagcgggatt cgatggactc aatgcacgaa tcgaccttcg tcttgccagg
     1861 gagaatctcg atcacgcgct cggacgagat gctgttcgcc catgaggcgg cttactcaga
     1921 atcgcgagta atcccgtatt ttttgagctt ttcgagcagc gtcacccgcg cgatgccaag
     1981 ccttcgcgcg gcctccgatc gattgccacg acattgtgcc aattcatgaa cgagaattcc
     2041 tcgttcgtag gcatccacac gctctttcag accgaaagaa gccttatctg cttcttccga
     2101 acgtggatgc atcaaccatg tcgcgtcggc aacacctccc gtggacaagg ccacggcgcg
     2161 ttccacccga tgctcgagtt ctcgcacgtt tcctggaaat ggccctgctt gcaggtgttc
     2221 tcgcaaggca gccgtgagtg tcaaaggtcc cgtggcaaat cgattttgat acatatcgag
     2281 gaaatgcgtg atcaacacgg gtacgtcatc catccgctct tcgagggggg gcaaacgaac
     2341 ctccacgaca tcgagacgat aatacaggtc ctctcgaaaa cgtcccgcgg cgacttcggc
     2401 tttcaggtcc ttgttggtgg tcgcgagtac gcgcacatcg acggaatcgt cccggtcttc
     2461 tcccacggga cgaacggttc gttcttgcaa tacgcgaagg agtttgcctt gcgtgctcat
     2521 gtccatttcg ctgatttcat cgaggagaat cgttccgcca tgggcttcgc gaaacagccc
     2581 tggtcgtgct cgcgacgccc ctgtgaaggc tcctttcgcg tgtccaaata gctctgcttc
     2641 cgccnnnnnn nnnnctcgga atggccgcgc aattgaagcg aatgaacggt gacgaggctc
     2701 gcggcgagcc cgccacgatg gcccccgcga tacgttcctt gcccgttccg cttggtcccg
     2761 tgatcagtac ggtcacatct ttcggagcta cgcgatacac gagaagagca atccttttca
     2821 tcgcttcgga cgcgaaaacc acgtgtcggg acaagagcaa ttccgcgcga agctgctcat
     2881 tttcttgctc cgcgcgcacc gatgccaccg cccgttccac cacccccaag acctcttccg
     2941 tatcgaaagg cttcgagaag tagtcaaaag cgccaagctt catcgctcgc accgcatgac
     3001 gttccgagcc atgggccgtg agcaaaatcg ttttgggcgc aggaggggcc gcttgaagac
     3061 gctccaatag ctgcaaacca tcgagccggg gcatctgcaa atccgtgatc accaaatccg
     3121 cacgatgcgt ttgcaaccac cccaaggcct caaccccatc gcgagcctcg attacgtcca
     3181 tctccgcact ctcgaggatt tccctgagcg tgtagcgtac ccctgtatca tcatccacga
     3241 tgagaactcg cggtttcaac gtagtgcctc ccctgcattc atcgtatcct gggcgtgacg
     3301 cggcagtacg atggtggctt ccgtgcctcc cgtggggcca tggttcaacc tcagcgttcc
     3361 accatgttgc tcgattacgg agcgagccac gatgagtccg atgccggtac cgcgttcctt
     3421 ggtggtgaac ccaggtgtaa atatttgatt atattggtgt ttatcaatcc ccggcccttg
     3481 atctcgcacg cggatcgata ccgtttccga tgactcggag atgtcgattc gaaccgtcgt
     3541 tccctggggg ctcgcttcca gcgcattttg cacgagattg accagcgcct gttccacttt
     3601 ggaggcgtcg cattccacga cgatgggggt attggtttcg agcgtaagcg acacacctcg
     3661 ccgaagggcc acgccttcat ggagagtgac cacgctttgc accagctcgg tcaaggcgac
     3721 ggagccgcgg ctcagtccgc tcaacgggcg tgtcaagttg cggaactcgt cgagtacggt
     3781 cgacattctt tgaatttcgc gcagcatgac atcggttctt tctttgtctt tggtgcccgg
     3841 ttccatacgg cgcgccatca actgtgtcag tccctggatg gaagataagg gatttttgag
     3901 ttcgtgggcg atggtaacgc ccgtggataa tagctcccga tttcggcttt ctaaggtatc
     3961 caacgcatgt tgtcgaatct gaatggcttc gcgcaccacc cgctcgaagg ccgcgcgcgc
     4021 cgtgtgggtg gcgatcgtgg tgatgatgag aacgacgaaa agcatggctg ctcgtgccac
     4081 cagccatgcg gcacctttca ccggcgagtc ctgcgggtga aggaacgccg gaatcatcga
     4141 gggcagcagg tcggttacga cgcccaaggc cagtagcgtg atgatgacaa atggcacggt
     4201 gaccacggaa accagtcgcc atcgttgggt caagccgagc cccgccaaag ccacgagagg
     4261 aacgaatacc accagcattg gactggtgaa accaccggtc acgtaaacga gcaccgattg
     4321 gattcccaac gcgaacaaca aattgatttg aacggtgccc tcgttggcgc gctgatgccg
     4381 caatcgtcgt cgttcgaagg cgaacagcac tccgaccgcg atgacactgc cgaagatgag
     4441 ccatactttc caaggtgtcg tatcgaaaag cagaaacacc aacgctacga tcagcagcaa
     4501 aggaagcagc agcattcggg cgcgaaagac gcgtcgaacc agttcgtcca tctcttgctg
     4561 ccacagttca tgcgcggcat atcgaatgct gtggggagct tcggtccgat cgtcggtcac
     4621 cgtttttctc cttgttgtga gtctgacgaa cttggctgga agtccgacag gctgtcagct
     4681 tttcgtacat gggggcgatg ggcaggggcc atggttaatc cgagatcacg attattcttg
     4741 ggtgaatggt tcgccgttgg ggaagcgaga agtgtgcccg gttcagtgtg cccgatggaa
     4801 ttgtggcaat gcaacgtttg ccgttcgtgt acagtgaacg acatgcgcgc gatggtgctc
     4861 gaggctcctg gttcaccgct tgtgcagcgc ttgttgccta atcctgttcc ttccgcaggg
     4921 caggtgttgg tggacgtatc ggtatgtggc atttgccgta cggatttgca tgtcgtcgat
     4981 ggggaactca cggaaccgaa actgccggtc attccagggc atcaggtcgt gggtcgcgta
     5041 gccggatggg gtgcgggggt aacgggtttt tctttgggtg atcgcgttgg gattccgtgg
     5101 ttggggggct gttgcgacgc gtgtgaggct tgtattcgcg gtcaagaaaa cctttgcgat
     5161 gcgtctcgct ataccgggta tcaaatcgat ggcggattcg cggagcaatg cgtggcggac
     5221 gcggggtttt gttttccgat tccggaaggg tacccggatt tgcaggcggc gccgctatta
     5281 tgcgcgggat tgattggata ccgggcgcta ggcatggcgg gggaaggtca caagcttgga
     5341 ttgttcggct tcggtgccgc ggctcatatt atcgctcaag tggctcagta tcagcagcga
     5401 caggtctatg ccttcacacg cgatggcgat acgagcagtc agcaattcgc gagaagtctg
     5461 ggggccgtgt gggccggtgg cgtttccgac agccccggtg tcatgctcga tgcggccctg
     5521 ctattcgccc ccgtgggcag tttggtgcct gcatcgttga agtcggttcg aaa
//
LOCUS       DAOSOR010000398         5570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14720_length_5570_cov_3.759383, whole genome shotgun sequence.
ACCESSION   DAOSOR010000398 DAOSOR010000000
VERSION     DAOSOR010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14720_length_5570_cov_3.759383"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="PK710_18120"
     CDS             <1..893
                     /locus_tag="PK710_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11700.1"
                     /translation="ASPLMAYFTEPADDYSTAVGYVKSLKNGKTVSLGRIGYHVESPR
                     FGLSGEWVVFSRNLDGNYSGDLWVWDAANDPWQGQVLGTEIAKNAFFPYMVMTAQGLL
                     FGTDATQESMTLRYWSLSKRETVVLGANLFGEPVLSDDGNKLLFLTNFDNWLADLFYV
                     KLADPTPIPLGKKVHYYSMWVDADWNYLACTTDGKGDLGTPTVIDLQTQEKTTLGKDT
                     PFFGIFPCPDGHGFSYLHDWDLAHGNLSTTTFAEPSTAVPIDENATETVLPSNRRLMY
                     VVQGDAERAGIYVAPLPSSP"
     gene            complement(1081..1644)
                     /locus_tag="PK710_18125"
     CDS             complement(1081..1644)
                     /locus_tag="PK710_18125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11701.1"
                     /translation="MDRKYKILIGITYLALAGCSGTTDVLEGPDSNEGTVVSQNSAVS
                     DCGGFTSSGQQASNYCDAEKLLWSFDKAAGVLSLVNTRVVLNCCGEHSFDVVRGTTGV
                     YDALETDTVGGQMGRCRCMCSLDFRTEIKDVSSTSIDLVLRRHITDEGAAKEIWAGTL
                     DLRTATGEVIIDGTPVEFGCKDQGTER"
     gene            complement(1688..2404)
                     /locus_tag="PK710_18130"
     CDS             complement(1688..2404)
                     /locus_tag="PK710_18130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11702.1"
                     /translation="MAGQGKQSGWWIVASVIAVMACENAISPQPEPPGRGILSIDPSG
                     IQSWIEPDSKGFVGLRGAPGCAKPAGATVRATPLYTPQDPNQSVIHADGSFEIPLAME
                     LGDEVRLQIHTDFLRSEPLDLQVAGHGVTMNPVSHPLACLTLVPSSELDLTSGSETVL
                     AINGCSTPVVLETPTFRRSTQSIRITGTSWPVTLQPEKTWEISFESDESPSGFEEIVF
                     LPIASPQRDRRAITLFASPK"
     gene            2568..>5570
                     /locus_tag="PK710_18135"
     CDS             2568..>5570
                     /locus_tag="PK710_18135"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT11703.1"
                     /translation="MPFQPGELLLHRYRISHILGHGGAATTYRALDLVWNLPVVLKIL
                     HHTSPTLRATLQAEFLVLRGLSHPNLIRVHDFAYIQNPKGNPLCLMSTEPIDAIPLDH
                     AAHHASWKTLEPAIADVLEALRCLHSLGFRHGDVKPSNILVRPDRRAVLIDLGCTGRI
                     GQASPLLFGTRGFLAPELMAGQPADGRADLFSLAKTIDALRPHLASALPAPVDALLER
                     MTDPNVENRPCSVEEVLETLGISPTQTARTLGRNGVLAGREKLLLTLRTQLDDLIGQR
                     PSSRIVWLTAEKGAGKTRLLQEIKWEAQLRARVFEVNCHAGSAVHDALSLATGSPLSR
                     QSIDAIVQAHEQLEAEALPTVFVIDDADRLSEEQATALTVLLRSIRSTSSSLAVVSST
                     QPPGETLEAISLSLEPLDLEALHAWIGQSLPDNAIQTIHLLSEGLPASVRYLLDRLEL
                     DDLSIEKLLVSGPQFPTSSYRIEKAHALSLEAKRLLGMLIIRGEGLSREQLESFGISL
                     QAANELVKASLAESEPFGWKLRRLGEADSLLCVVGDDVVRDLHRQAALVLDRSEAQTE
                     TKASERNARAVYHLAHAGDWQEARDRLMDMQHLFDGAPRSWHRAASAVLRGQTDPELE
                     LLMARLEQQVGDGQRAWTRLWTLSQTASKPQLLRIKEELATCATALGRLDDAIVWFEA
                     ARADAEKPDQHAALSFQLARALTRKGHYRDALHVCEKSLQDVQTEELRSDLLEAAAVA
                     RSYLGDAQGARNLFERTASDDRKDDNPRRRVRSLGSRALVDYRAGHLEAAAQGYSLAL
                     QHAQTHGLLDMVATAALNLGTLCHQQGNWAAAVKSYHQGMRMAVALGQSGTETVLRFN
                     LAKLYSDVGQFERAKTMAEQCLMACRRAAQPLMEAAAQTVLGEVATAQRDFERAKIAF
                     QTALTAYETQESQRECAEILLQLADVALLQTDLDDATKLLQRARSCLVDLDARDVQIR
                     AELIQSRLDLASGKGDQALATAERVVPQA"
BASE COUNT         1159 a         1706 c         1494 g         1211 t
ORIGIN      
        1 aagcatcgcc cttgatggct tattttaccg aacccgccga tgactacagc accgccgtgg
       61 gttacgtaaa gtctttgaaa aacggcaaga ccgtgtcatt gggccggatc gggtaccacg
      121 tggagtcgcc gcgttttggt ctgtcggggg agtgggtggt cttttctcgc aacctggacg
      181 gcaattattc cggtgatttg tgggtttggg atgctgcgaa cgatccctgg caaggacaag
      241 tgctcggaac cgagatcgcg aaaaacgcct ttttcccata catggtcatg acggctcagg
      301 gcttgctctt tggtaccgat gccacgcagg aaagcatgac actgcgctat tggtcacttt
      361 ccaaacggga aaccgtcgtg cttggcgcca acctgttcgg cgagcccgtc ttgtccgacg
      421 atggtaataa gctcctattc cttaccaact tcgacaactg gctggcggac cttttctatg
      481 tgaaattggc tgacccaacc ccgattcccc tgggcaaaaa agtgcattat tacagcatgt
      541 gggtggatgc ggattggaat tacctcgctt gtactaccga tggaaaaggc gacctgggga
      601 ccccgaccgt catcgatttg caaacccagg agaagacgac tctcggcaag gatacccctt
      661 tcttcggtat tttcccctgt cccgatggtc atggcttctc ttacttgcat gattgggacc
      721 tggcccacgg caacctttcc acaactacgt ttgccgagcc atcgacggcc gttcccatcg
      781 atgaaaacgc tacggaaacc gttttgccct ccaatcggcg cctcatgtat gtcgttcagg
      841 gggacgctga acgcgccggc atctacgtgg ccccgcttcc ctcttctccg tgatcggtgg
      901 gctccatcat ccacccgact gatcgcggat tcctgattcc tctccttcgg ttccccgcac
      961 cagaccccat cacgggcgaa atcgatggca aagacgccac acaacgcaaa aatgccgtca
     1021 aagagtgcct ttcagcgaaa aagaacggtt cgtcagccgc cctagagcca attccatccc
     1081 ctagcgctcg gtaccttggt ccttacaacc gaattcgacg ggcgtaccgt cgatgatgac
     1141 ctcccccgtg gccgtgcgca aatcgagcgt tccagcccaa atctccttgg ccgctccctc
     1201 gtcggtgata tggcgccgca acaccagatc gatggatgtc gacgaaacgt ccttgatctc
     1261 agttctgaaa tcaagactac acatgcaccg gcatcgtccc atctggccgc cgaccgtatc
     1321 cgtctcgagc gcgtcgtaga cgcccgtggt gccgcggaca acgtcaaagg aatgctcgcc
     1381 gcagcaattg agaacaacgc gggtattgac gagggataac acgcctgcag ccttgtcgaa
     1441 ggaccagagc aacttttcgg catcgcaata attcgaagct tgctgcccgg atgaggtgaa
     1501 acctccgcaa tcgctgaccg cgctgttctg actcacgacc gtaccctcgt tcgagtccgg
     1561 accttccaga acatcggtgg tgccactgca tccggcgaga gccaagtatg tgattccaat
     1621 aagtattttg tatttgcgat ccattgtctt gctccttgtg ggctaccttg tttgtgggtt
     1681 tcattcccta cttgggactg gcaaaaagtg tgatcgctcg acgatctcgt tgcggagatg
     1741 cgatgggcaa aaacacgatc tcttcgaatc cggaagggga ttcgtcgctt tcgaaggaaa
     1801 tctcccacgt tttttcaggt tgcaacgtga ccggccacga cgtgccggtt atgcggatgc
     1861 tctgggtgga gcgtcgaaat gtgggtgttt cgaggaccac cggggtggag catccgttga
     1921 ttgccagtac ggtttcggaa ccggaagtca aatcgagttc cgacgagggg acgagtgtca
     1981 ggcaggcaag gggatgggat accggattca tggtaacgcc atgacccgcc acctgaaggt
     2041 ccagaggctc ggagcgcagg aaatcggtgt ggatttgcaa gcgcacctca tcgcccagct
     2101 ccatcgcgag cgggatttcg aagctgccat ccgcgtgaat cacggattga tttgggtctt
     2161 gcggggtgta aaggggagtt gcgcgaacgg tggcccccgc tggcttggcg caaccaggcg
     2221 ctccccgaag tccaacaaat ccttttgaat ccggttcgat ccaggattgg ataccgcttg
     2281 gatcgatcga taggatgccg cgccccggcg gttcaggttg cgggctgatc gcgttttcgc
     2341 aagccataac cgcgatgacg ctcgccacga tccaccagcc tgattgtttt ccttgtcccg
     2401 ccatcttacc tcttccggtt agcaagaccg atgccagtca tttccaattt gggatttgcc
     2461 cagttttttg ctgctccccg cgaaatctat tgtgccaata ggccaaaaat gtgtttcaat
     2521 atgtatcatt gtgatttgtt gtgatcggtc aatcctgagt ctgtaccatg ccattccaac
     2581 ccggtgaact gctcctgcat cgctaccgca tctcccatat cctcggccac ggtggcgccg
     2641 ccacgaccta ccgcgccctc gacctcgtct ggaatctgcc cgtagtcctc aaaatccttc
     2701 accacacctc cccaaccctg cgcgccaccc tgcaagccga gtttctcgtc ctgcggggac
     2761 tctctcaccc caacctcatt cgcgtccacg acttcgctta tatccaaaac cccaaaggaa
     2821 atcccctctg cctgatgtcc acggagccga tcgatgccat ccccctcgac cacgccgccc
     2881 atcacgcctc ctggaagacc ctcgaacccg cgattgccga tgtcctcgaa gctctgcgat
     2941 gcctgcattc tctaggtttt cgtcacggcg acgtgaaacc ctccaacatc ctcgttcgcc
     3001 ccgaccgacg cgccgtactt atcgacctcg gttgcaccgg tcgaatcggg caagcatcgc
     3061 ctctcctttt cggaacccgt ggatttctcg ctcccgaatt gatggccggg caacccgccg
     3121 atggcagagc cgacttattc tccctcgcca aaaccattga tgcgcttcga ccccatctcg
     3181 cctccgctct tccggctcct gtcgatgcct tgctggagcg aatgacggac ccaaacgtcg
     3241 aaaatcgtcc ctgttccgtg gaagaagtcc tcgaaaccct gggtatttcc ccaacgcaga
     3301 ccgctcggac cctcggtcga aatggcgtcc tggctggacg tgaaaagctt ttgctcaccc
     3361 ttcgtactca attggatgac ctgattggcc aacgcccctc ttccagaatc gtctggctta
     3421 cggcggagaa aggcgcgggc aagacccgtt tgctccagga aatcaaatgg gaagcccaac
     3481 tgcgagcgcg cgtcttcgag gtcaattgcc atgcaggctc ggcggttcac gatgcgttgt
     3541 cgcttgccac gggctccccc ctctctcggc aaagcattga tgccatcgtg caagcccacg
     3601 agcaactcga ggctgaggcg cttcctaccg tcttcgtcat cgacgatgcg gatcgcttgt
     3661 cggaagagca agccaccgcc ttgaccgtct tgcttcgaag catccgttcc acgtcgtcat
     3721 cgctggccgt ggtgtcttcc acgcagccgc ctggcgaaac cctcgaagcc atctcgcttt
     3781 ccctcgaacc tctcgatctg gaagcacttc atgcctggat tggccaatcg ttgcccgata
     3841 acgccatcca aaccattcat cttctcagtg agggactgcc agcctccgtg cgctatttgc
     3901 tcgatcgtct cgaactcgac gatctttcca tcgaaaaatt actggtatcg ggtcctcaat
     3961 tccccacatc gtcgtatcgc atcgaaaaag ctcacgcgtt gagcctcgaa gccaagcggc
     4021 tgttggggat gctcatcatc cgaggcgagg gactatcgcg cgagcagctc gaatccttcg
     4081 gtatttccct gcaagcggcc aacgaactcg tcaaagcctc cctggcggaa tccgaaccat
     4141 tcggatggaa attgcgacgg ctcggtgagg cagacagctt gctttgcgtg gtgggcgatg
     4201 acgtcgtgcg agatctgcac aggcaagcgg ctctcgtgct ggacagaagc gaagcgcaaa
     4261 ccgaaaccaa ggcaagtgag cgcaatgccc gggccgtata tcacctcgcc cacgctggtg
     4321 attggcagga ggctcgggat cgcctgatgg acatgcagca tttattcgat ggcgcacccc
     4381 gttcatggca tcgagccgcg agcgctgtgc tccgtggaca aaccgatccc gaactcgaac
     4441 tgctgatggc gcgtttggaa caacaggtcg gtgatggaca acgggcctgg actcgtttgt
     4501 ggaccttatc ccaaaccgcc tcgaagccgc aattgcttag aatcaaagag gaattagcta
     4561 cctgtgccac cgcccttggc cgtctcgatg acgccatcgt ctggttcgaa gcggcgcgtg
     4621 cggatgccga aaaaccggac caacacgccg ctttatcgtt tcaactcgcg cgcgctctca
     4681 cgcgaaaagg ccattatcga gatgccttgc acgtctgtga gaagagcctg caggatgtgc
     4741 aaacggagga attgcgttcg gacttgctcg aagcggctgc cgtggcgcgc tcctatctcg
     4801 gtgatgcaca aggggcgcgg aatttgttcg aacggacggc gagcgacgat cggaaggacg
     4861 acaacccgcg acgtcgggtt cgctccttgg gtagcagagc ccttgtggat taccgagccg
     4921 gtcacctcga agctgctgcg caaggataca gtcttgcgtt gcagcacgca caaacacacg
     4981 gactgttgga tatggtggcc accgctgctt tgaatctggg taccctctgc catcaacagg
     5041 gcaactgggc agcggccgtg aaaagctatc atcaaggcat gcgaatggcc gtagccttgg
     5101 ggcaatcggg cactgaaacc gtcctgcgct tcaacctcgc caaactctat tcggatgtcg
     5161 gccaattcga gagggccaaa accatggccg aacaatgcct gatggcttgt cgtcgcgccg
     5221 ctcaacccct gatggaagcc gccgcgcaga cggtgctcgg cgaagtcgcc acggcacaaa
     5281 gggatttcga acgcgcgaaa atagcttttc aaacggcgct tacggcctac gaaacccaag
     5341 aaagtcagcg agaatgcgcg gaaatcctgc ttcaattggc ggatgtcgcg ttgctgcaaa
     5401 ccgacctcga cgacgccacg aagctgcttc aacgtgcccg ctcttgtctc gtcgacctcg
     5461 atgcgcgcga cgttcagatc cgcgccgaat tgattcaatc gcgcctggat ctggcctcgg
     5521 gaaaaggcga tcaggccctg gcgaccgcgg aacgagtcgt gccacaagct
//
LOCUS       DAOSOR010000399         5555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14766_length_5555_cov_4.627091, whole genome shotgun sequence.
ACCESSION   DAOSOR010000399 DAOSOR010000000
VERSION     DAOSOR010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14766_length_5555_cov_4.627091"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /locus_tag="PK710_18140"
     CDS             <1..1319
                     /locus_tag="PK710_18140"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT11704.1"
                     /translation="RVFGHFPVRIGRNPLNDLRLVPGFVSQFHAVLELHGNTLMLRDL
                     GSKNGTTLSTGQAAAHELVDLAESDGKFSISALSFSAAVEDVGEVVPARAPQRGWLLG
                     GLGTDSHSMAETMLLDPSQGVPMGRPSSDSISEKPTDGTPSLRSQIKAYQSSRGTISR
                     VLQTLASELSPMTPEQSQALLGWARDNFPGINEEPEFKRLVEHHRAVVDDREASKQSR
                     LEYVALQAVRELSSLYNPRNSLGTEIDLIQFLTRVRDTLDMFFRTFIPLRDGHRQFKS
                     EMDIRPATPYLDSPAARAAAGVSRAKTPQALAELLLGSADEQMAAQKAIEGTFADLMI
                     HQIALLSGVMRGVHALMQELAPSTVEAQLEERRRKGDTGWMFGPYRFRELWKVFIARH
                     ADLTESEKQLYSHVFGHDFIRAYAQLSGEAGSHVPSESDRNRPHEG"
     gene            1322..1987
                     /gene="tssJ"
                     /locus_tag="PK710_18145"
     CDS             1322..1987
                     /gene="tssJ"
                     /locus_tag="PK710_18145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HOT11705.1"
                     /translation="MGAYSRWGSVHAWFETLGILTCLQMASCGSTIVPVREPKKQCEI
                     QNITLSVIASDTINPSPEGSARPVVLRVYQLKDEIAFVNATYEDVWRKDQQVLGASLV
                     VRGETYAYPNTRTEIAFQRNPEADSLVIAALFRDYQGKSWFISFELPPAPGKGDCHIE
                     GCEGDSCGIDFNPRYAVWLDDIRVEEGSQHLQDVTEQHRVRVVQLGKTAPQAPPKGDA
                     SKP"
     gene            1984..3336
                     /gene="tssK"
                     /locus_tag="PK710_18150"
     CDS             1984..3336
                     /gene="tssK"
                     /locus_tag="PK710_18150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HOT11706.1"
                     /translation="MIHKLVWTEGLFITQHHFQQLDRYHEALLGERLRAALAWEWGVT
                     HLEIDERALSSNQLLVSHLSAVLPDGTALDCHEGKPDAIPPRSFASEFSPQLQSLDVS
                     VALVHEMLGATNVDLDGSSIHVARYARKQEYVVDANTGTGEQPVDRAHPLIRLLFGDE
                     VKGSFDAIRIGQLVRGPTGSVQLRPSCVPPCLRVSASPWLLQGFRRLLTIMTARQRSF
                     AQARRQRTAGSIEVDASDTLRFLFLDVLNTNIPIFSHIVDWGTVHPEQAYLVISGLVG
                     RLCSFTDVDPTTIPKFDYLDLGATFAPLFDMGSRMLESIISERYTEIELHRREDGVFV
                     GRATGEQIMSSDYFLAVSGTLPEAQVRERLPKMMKIASVNQIQAILKSAVNGAQLELE
                     YRPPSALPLKPGTTFFRLQRTPDFWPDIASTGTFALFHPFDPQVLTLKLYAIESDRTP
                     "
     gene            3333..4043
                     /gene="icmH"
                     /locus_tag="PK710_18155"
     CDS             3333..4043
                     /gene="icmH"
                     /locus_tag="PK710_18155"
                     /inference="COORDINATES: protein motif:HMM:NF038228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HOT11707.1"
                     /translation="MTSPSSPLQAVYWSCGEALALASHIGSSPDLPSAFELKQRFLSL
                     LEQVAEKATRAGLSREDMEDIRYAIVAFIDEQVLQSPWDGKQEWMMEPLQLEYFNETT
                     AGEGFFSRLAVIESRPERVHVMQVYYLCLVLGFQGVHAIKNPEALEARIETLAAKLSA
                     QLPAQDRISPHGIPADSGRQRATKSLPIIHVGVAIVLLSIVAYSALRIAIMASASDAA
                     STIQQTASSIPISPSEGK"
     gene            4050..>5555
                     /gene="tssM"
                     /locus_tag="PK710_18160"
     CDS             4050..>5555
                     /gene="tssM"
                     /locus_tag="PK710_18160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HOT11708.1"
                     /translation="MWLILLAVLLIAGGWVGGWLLGWPLWIEISLSALIVLVTVGIIV
                     FRRLRAIHAARALEREILAQAEKHAATARPDHRAEILALQDKMKQGIGALKKSKLAVA
                     SGGGALYSLPWYVIVGPPGAGKTTALRHSGLVFPFLDPSQDGIKGLGGTRNCDWWFTN
                     EAVLLDTAGRYATDDDDRDEWFAFLDILKKHRARTPINGMLVAISVTDLAGQPEERIE
                     QAAQILRARIDEVISRLQMVVPVYVVFTKMDLIDGFVEFWEDLRKSEREQMWGMSFEL
                     QRPKDFEPAKQFAKEFDLLTERVHARSIARMRTTRRAEARAKVLQFPLEFTSLKSDLA
                     LFLETLFQKNAYQETPLFRGAYFTSGTQEGRPLDRVMAGMASAFGLRLDTAPGTQTID
                     PKSYFLTEMFQKVVFPDRHLAVRTAVEARRRMWLQVGVGVGAVSLGAGIVIPGLIGYL
                     NNRTLIQDSLSETQQARLVDPNDRGELSSRIDALSHLRGHLDRLDRWEADGA"
BASE COUNT         1197 a         1539 c         1563 g         1256 t
ORIGIN      
        1 cacgtgtgtt tgggcatttc ccggtgcgca ttggcagaaa tcctctcaac gatctgaggc
       61 tggttcccgg gtttgtctcg cagttccacg cggtgctcga actgcatggt aacaccttga
      121 tgcttcgaga cctggggagc aaaaacggca cgacgttatc cacgggacag gccgctgccc
      181 acgaactcgt cgatcttgcg gaaagcgatg ggaaattctc tatttccgcc ctttcgtttt
      241 cggctgcggt agaggatgtg ggagaggtcg tccctgcccg tgcgcctcag cgaggttggc
      301 tgctaggcgg acttgggacc gacagccatt cgatggcgga aacgatgttg ctcgatccgt
      361 cgcaaggcgt acccatgggg cgtccatcgt cggactcgat ttccgaaaag ccaacggacg
      421 gcacaccatc gcttcgttcc caaatcaagg cgtatcaatc gagtcgtggc accatctcgc
      481 gcgtgctgca aaccctggct tctgagcttt ctcccatgac gccggagcaa agccaagcac
      541 tgctcggttg ggctcgtgac aatttcccag gaatcaacga agaacccgag ttcaagcgtc
      601 ttgtcgaaca ccatcgggcc gtggtcgacg acagggaagc gagcaagcag agtcgtcttg
      661 aatacgtggc attgcaggca gtacgggagt tgtcatcgct gtataatccg cgcaactccc
      721 ttggtacgga gatcgatctc attcagtttc tgacgcgtgt gcgggacacg ctcgacatgt
      781 ttttccgaac ctttatcccg cttcgggatg ggcatcggca attcaagtcg gagatggata
      841 ttcggccggc gacaccgtat ctcgacagtc cggccgcacg ggcggcagcg ggtgtaagcc
      901 gcgcgaaaac gccacaggct ctagcggaat tattgctggg ttcggcggat gagcaaatgg
      961 cggcgcaaaa ggcaatcgag gggacgttcg cagacctgat gatccatcaa atcgcattgc
     1021 tcagtggcgt gatgcgagga gtacatgcct tgatgcagga gctggcccca agcacggtgg
     1081 aagcacaatt ggaagaacgt cgccgaaaag gagatacggg ttggatgttt ggtccgtatc
     1141 gattccgtga gttgtggaag gtttttatcg ctcgtcacgc agacctgaca gaaagcgaaa
     1201 aacagcttta ctcccacgta tttggacatg atttcatccg ggcttacgcc cagctttcgg
     1261 gagaagcggg ttcccatgtt ccttccgaat cggatcggaa tcggccacat gagggataac
     1321 catgggggcc tactcacgat ggggttcggt acatgcttgg ttcgaaaccc tgggtatcct
     1381 cacatgcctg caaatggcat cgtgcggttc caccatcgtt cctgtccgag agccaaaaaa
     1441 acaatgtgaa atccagaaca taacgttatc cgtcatcgct tcggacacca tcaatccaag
     1501 ccccgaaggc tccgctcggc cggtggttct gcgcgtatat caactcaaag acgaaatcgc
     1561 attcgtcaac gccacgtatg aagacgtatg gcgcaaggac caacaagtgc ttggggccag
     1621 tcttgtcgtg cggggagaga catacgctta tcccaacaca cgaacggaaa tcgcatttca
     1681 gcgcaatccg gaagcggatt cgttggtcat tgcggcacta tttcgagatt accaaggcaa
     1741 aagttggttc atctctttcg aattgccccc ggcgccaggc aagggagatt gtcatatcga
     1801 gggctgtgag ggtgattcct gcggcatcga tttcaatccg agatacgcag tgtggctcga
     1861 tgacatccgt gtcgaagaag gctcccagca tctgcaagac gtgacggaac agcatcgggt
     1921 tcgcgtggtt cagcttggca aaaccgcccc tcaagccccc ccgaaagggg atgcgagcaa
     1981 gccatgattc acaagctcgt ttggacagaa ggcctgttca tcacgcaaca ccacttccag
     2041 caattggatc gctatcacga agctttgctt ggcgaacggt tgcgagctgc tcttgcgtgg
     2101 gaatgggggg tcacccacct agaaatcgat gagcgggccc tttcgtccaa ccaacttttg
     2161 gtctcccatc tttccgcggt attgcccgat ggtacggccc tcgactgtca cgagggcaag
     2221 cccgacgcca tcccgcctcg ctcttttgcg tccgagttct caccccagtt gcaatccctc
     2281 gacgtcagcg ttgctctggt ccacgaaatg ctcggggcca cgaacgtgga tctggatgga
     2341 tcgtcgattc acgtagctcg ttacgcacgc aagcaggagt acgtcgtgga tgcgaatacg
     2401 ggcacgggcg agcagcccgt ggatcgagca caccccttga ttcgccttct ttttggcgat
     2461 gaagtgaagg gttctttcga cgcaatccgc attggacagc tcgtccgtgg gcccacgggt
     2521 tccgtgcaac tcagaccgag ctgtgttcct ccctgcttgc gagtgagcgc ttcgccctgg
     2581 ctcttgcaag gattccgccg ccttctcacc atcatgaccg cgcgacagcg ttctttcgca
     2641 caagcgcgtc gtcaacgaac ggcaggcagc atcgaagtgg atgcctccga tacgttgcgg
     2701 tttctatttc tcgacgttct caacacgaac atcccgattt tttcgcatat tgtcgattgg
     2761 ggaaccgtgc atcccgaaca agcctatctg gttatatcag gcctggtcgg tcgtttgtgt
     2821 tctttcacgg atgtggatcc aaccacgatt cccaagttcg actatctcga tctcggggcc
     2881 acgtttgcac cgctattcga tatgggctcg aggatgctcg agtcgatcat atcggagcgg
     2941 tacacggaaa tagagcttca tcgtcgtgaa gatggggtgt tcgtgggacg ggcgacgggc
     3001 gaacagatca tgagttcgga ctactttttg gcggtttccg ggacattgcc ggaagcgcag
     3061 gttcgcgagc gattacccaa aatgatgaag attgcttccg tgaatcaaat tcaagcgatt
     3121 ttgaagtccg cggtcaatgg cgctcagctc gaattggaat accgcccccc ctccgcgctt
     3181 ccgctcaagc ccgggacaac gtttttccga ctgcaacgaa cacctgattt ttggccggat
     3241 attgcatcca ccggaacttt tgcgttgttt catcctttcg acccgcaagt gctgacgctc
     3301 aagttatacg ccatcgaatc ggatagaacg ccatgacatc gccttcctca cccctgcaag
     3361 cggtttattg gtcgtgcggg gaagcgctcg ccttggcctc gcacatcggt tcatcgccgg
     3421 atcttccttc ggccttcgaa ctcaaacaac gattcctatc gttgcttgaa caggtagccg
     3481 aaaaagcaac gcgcgcggga ttgtctcgcg aagacatgga agacatccgc tatgccatcg
     3541 tggcgttcat cgacgagcag gttctgcaat cgccgtggga cggaaaacaa gaatggatga
     3601 tggagccgtt acagctcgag tacttcaatg agacgacggc tggggaggga tttttttcgc
     3661 gacttgcggt gattgaatct cgtccggagc gagtgcatgt catgcaagtt tactatttat
     3721 gtctcgtgct tgggtttcag ggtgtgcatg ccatcaagaa ccccgaggct ctcgaagcgc
     3781 gcatcgaaac tctcgccgcc aagctttccg cacaactacc tgctcaagac aggatcagtc
     3841 cccacggcat tcccgccgat agcggtcgtc aacgcgccac caagtcgttg cccatcatcc
     3901 atgttggggt cgccatcgta ttgctttcta tcgtggcata ttcagcctta cggattgcca
     3961 tcatggcgtc ggcttcggat gcggcatcga cgatacaaca aacggcctcc tcgattccca
     4021 tcagtccttc ggaagggaag taaacaacga tgtggctcat cctgctcgca gtattgttga
     4081 ttgccggagg ttgggttgga gggtggcttc tgggatggcc gttatggatc gagatctccc
     4141 tttcggcctt gatcgtcctt gtcaccgtcg ggatcatcgt ttttcggaga ctgcgtgcga
     4201 ttcacgcagc gcgggctctc gaacgcgaaa tcctggcaca ggccgaaaaa catgccgcta
     4261 ccgctcgacc ggatcatcgg gccgaaattc ttgcccttca agacaagatg aaacagggaa
     4321 tcggcgccct gaaaaaatcc aaactcgccg tcgcatccgg tggtggcgcg ctctattcct
     4381 taccctggta tgtgatcgtg ggccctccag gtgctggaaa aaccacggct cttcgtcact
     4441 cgggccttgt gtttcctttt ctcgatccga gccaggatgg catcaagggt cttggtggca
     4501 cgcgcaattg cgattggtgg ttcaccaacg aggcggtgtt gctcgacacg gcggggcgat
     4561 atgccactga cgatgatgac cgggacgagt ggtttgcttt cctcgacatc ctgaaaaaac
     4621 acagggctcg cacgccgatc aacggcatgc tcgtggccat tagtgtcacg gatctggctg
     4681 ggcaaccgga agagcgaatt gaacaagccg cccagatact gcgtgcgcgc atcgacgaag
     4741 tcatctcgcg cttgcagatg gtggtgccag tgtatgtggt gttcacgaag atggacctca
     4801 tcgatgggtt cgtggagttt tgggaagacc ttcgaaaaag cgaacgcgag cagatgtggg
     4861 gaatgagttt cgagcttcag cgccccaagg acttcgagcc agccaagcaa tttgccaagg
     4921 aattcgacct gttgaccgaa agggtgcatg ccagatccat agcgcgcatg cgaaccacgc
     4981 gtcgtgccga ggcacgagca aaggtgcttc agtttccgct cgaattcact tcgttgaaaa
     5041 gtgatctcgc gctgttttta gaaacgctat ttcaaaaaaa tgcctaccaa gaaacccctc
     5101 tgttcagagg agcatatttc acgagcggaa cccaggaagg ccgtcccctc gaccgcgtga
     5161 tggcgggcat ggccagtgct ttcggactgc gcctcgacac ggcccctgga acgcaaacca
     5221 ttgatcctaa aagctatttt ttgacggaga tgtttcaaaa ggtcgtattt cccgaccggc
     5281 atctcgccgt tcgcaccgcc gtggaagcac gtcgacggat gtggcttcaa gtcggcgtcg
     5341 gcgtgggtgc cgtaagtctc ggcgctggca ttgtcattcc aggattgatt ggatacctca
     5401 acaatcgaac tctgattcag gacagccttt ccgaaacgca gcaagcacgg cttgttgacc
     5461 cgaacgatcg gggcgagctt tcttcacgca tcgacgcgtt atcgcattta cgcgggcacc
     5521 tggaccggct cgaccgctgg gaagccgacg gagcg
//
LOCUS       DAOSOR010000400         5553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14773_length_5553_cov_3.567479, whole genome shotgun sequence.
ACCESSION   DAOSOR010000400 DAOSOR010000000
VERSION     DAOSOR010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14773_length_5553_cov_3.567479"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PK710_18165"
     CDS             <1..413
                     /locus_tag="PK710_18165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11709.1"
                     /translation="ARLGRASSESMVFLDSCFSGAGGRSVLPSGTRPLVRVTEPSPQA
                     HVAVLSASSVTEVAGQLPGGTSGLFTHFLLEGLGYGRADRDGDGQITLGELEAYVRPL
                     VSKAARRDHRDQTPVLSVEGRLSSAVVVSGVNAK"
     gene            complement(423..911)
                     /locus_tag="PK710_18170"
     CDS             complement(423..911)
                     /locus_tag="PK710_18170"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOT11710.1"
                     /translation="MSSMCDFNAILPAPFGALGVRHAHGKLIGLVFLPPETLPSSPRD
                     AFSERVSEQLEAYYANPEHEFGVPLEPKGTPFRMRVWRALQSISCGNTTTYGAIARDL
                     GSAPRAVGQAVGDNPLPIVIPCHRVIASDGSLGGFSHSRIGYTLEIKRWLLRHEGVAV
                     QG"
     gene            complement(1072..2331)
                     /locus_tag="PK710_18175"
     CDS             complement(1072..2331)
                     /locus_tag="PK710_18175"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11711.1"
                     /translation="MSRVSIIGAYNTKFGAFVQRDKETKQVTDSLSFYDLITEAGRGA
                     IADAGLTADQIDAVWVGSCSPSLFVNQEHVAPLAAEIDPHLRFKPMTRTECACASSSA
                     ALYDALYSLEANRYRHVLVLGVEKMNLLGTREMTHALSCSSHWPTEGSQGMSFPMLFA
                     AYAKGYQKRYGLADDELRRMFATVAALCYKNGAENPLAHFGPGGVAERAGLFTAEAIL
                     ALPAEGKGSNPMIADPLRLHDCSLVSDGAAALVLTSTDNALKTRDKVVEIAGIGHAVE
                     RMPENVRENMHELMAGKHAVHKAFEEAHVTIRDVDFAEVHDCFTINQLLSTEALGLSD
                     DGRAGHDYLDGRFTRDDRCPINLSGGLKAKGHPVGATGASMHALAYKQLMGEPIGVAA
                     RDPKVGVVFNVGGSAVSNFVTVLRRIR"
     gene            complement(2468..3358)
                     /locus_tag="PK710_18180"
     CDS             complement(2468..3358)
                     /locus_tag="PK710_18180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11712.1"
                     /translation="MRRFIPVFGLFAIVLSACAADNDTMPIRHESYDGGVSEPLQCIP
                     NLDGVVESWEMAPKLQQVASYIVTPPLPATATQGTYVNTEGVVNDQGKRIWNWSADDT
                     THLVAKIVAEPLTTQWYANAFVGAHFALPSDLSGSLVGIYSHDEQALRLHGVASVLEN
                     PPEGKTLLVYEKPIDFFPFPLMVGKAWTQTGIVKDGTLRGLSPWSQEDVYEVSVDASG
                     ELRLPDFTFTQALRVSTKVTAKPKAGSQQGYSQRQVSFVFECFGEVARATSMLFIDGV
                     TDDPGKDFTQAREIRRLGWF"
     gene            complement(3362..4945)
                     /locus_tag="PK710_18185"
     CDS             complement(3362..4945)
                     /locus_tag="PK710_18185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11713.1"
                     /translation="MSRSFLPISAALAAFCVASGAFATGFTDHGQDIVPTTETRLVLG
                     GAFRVRSEALYNLDLDRGPTPSGQVFFPVPVSDPKAQWLTYHDVRLRTDFAAYAPGGV
                     VAVKARVDILDNMPLGGSADGIPGGTVTQQPPSAAFRIKRAWGEVVTPLGYLAAGRMG
                     HHWGLGMLGNSGDCADCDSGDAADRITFATAAASHILALAYDFSASGHIVGRGDQVRS
                     IDIEPSAAVRSMSVALLNWRDDAARRRRAKAGKATFEYGGLYARRWQDKDIPATYLPI
                     VAGPTAIDAGQVMGRGLTANVFSLWSRFTVYGFRVEVEGAYSTANFDQASLIPGLEMN
                     HKVKARQFGGALESEIGQPNGLAGGGFDMGYASGDSAPGFGARPPLAKLTAPQPGDLD
                     GPQGLPPYDFRVDNFRFHPDYRIDRILFREIIGTVTDAVYLRPHARIRLLDFDTARLQ
                     ASVAGIASFANYASSTPGGEKYLGFELNPTLVYTSDDGFAAAFEHAVLFPGAGLNNRD
                     LGLTAKPAQLYRLRLSFGF"
     gene            complement(4942..>5553)
                     /locus_tag="PK710_18190"
     CDS             complement(4942..>5553)
                     /locus_tag="PK710_18190"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT11714.1"
                     /translation="SILAQARRVVALADQRGVKDFTVVGHSWGSSVSLAVAIAAPTRV
                     KRVALYNGMYFEEQKPTLFKWAQTPVLGEIIYGVFYPDRQDEKLSFAYYEPEKHITEQ
                     VVEDVQGLLNRPGTLAAALAGVRAMRYSELQKRYPTVHQPVLLMWGREDAVTPLTYGE
                     RLLQQLPRARMVVYPRCGHLPMLEVPGASTQELLRFLAEEQPS"
BASE COUNT         1246 a         1574 c         1606 g         1127 t
ORIGIN      
        1 cggctcgtct cggtcgcgcc tcgtcggaat caatggtgtt tttggactcg tgtttttccg
       61 gggctggggg tcgctccgtg ctgccgtccg gcacaagacc cctcgttcgc gtgacagaac
      121 cctcgcccca agcccacgtg gccgtgcttt cggcttcctc agtcaccgag gttgccggtc
      181 aactccccgg cggaacctca ggtttgttta cgcatttcct gctggaagga cttggctacg
      241 gtcgcgcgga tcgcgatggg gacggtcaaa tcacgcttgg agaactggag gcgtatgtcc
      301 gtccgctcgt ttcgaaagcc gctcggcgag accaccggga tcaaacgccc gtgctttccg
      361 tggaaggacg tctatcgtcg gcggtggttg tgtctggcgt gaacgcgaaa tagctattgc
      421 gcttagcctt ggacggctac gccttcatgt cgtagaagcc agcgtttgat ttccagcgtg
      481 tatccaattc ggctatggct gaaacctccc aagcttccat ccgaggcaat cacccgatgg
      541 cagggaatga cgatcggtaa ggggttatcg cccaccgctt gtccgaccgc gcgcggcgcg
      601 ctgccaagat ctcgcgcgat agcaccataa gttgttgtat ttccacaaga aatactctgc
      661 aacgccctcc agactcgcat tcggaatggc gtgcccttgg gttcgagggg tacgccgaat
      721 tcgtgctcag gattggcata atacgcctcg agttgttcgc ttacacgctc gctaaacgca
      781 tcccgagggc ttgaagggag cgtctctggc ggtagaaaca ccaagcctat aagctttccg
      841 tgggcatgac gcactcccaa cgcaccgaaa ggcgctggca aaatggcatt gaagtcgcac
      901 atcgaagaca tggcagcatt gtcgcctgtt ttgagacaaa gaaaaggatg atgaaaaccg
      961 acgacgcgca aacgagcgtt tccaccgata gtatgatctt ggcgtcggac cccacatttc
     1021 accatggcga tggggtgagg gatagggcga cgaagggaaa cggcgaaaaa gtcaacgaat
     1081 tcggcgaagt acagtgacaa agttactgac ggccgagccg ccgacattga aaacgacccc
     1141 aactttcggg tcgcgggccg ctaccccgat gggttcgccc atgagctgtt tataagcgag
     1201 cgcatgcatg gaagctcccg tggccccgac gggatgtcct ttggctttga gaccgccgga
     1261 cagattgata ggacatcgat cgtcgcgggt gaatcgtcca tcgaggtagt cgtggcccgc
     1321 gcgaccatca tcggataacc ccaacgcttc ggtcgacaga agctggttga tggtaaaaca
     1381 atcgtggact tcagcgaaat ccacatcgcg gatcgtgacg tgggcttctt cgaacgcttt
     1441 atgaaccgcg tgtttaccag ccatcagctc atgcatattt tcgcgaacgt tttcgggcat
     1501 gcgttcgacg gcgtgtccga tgccggcgat ttccacgact ttatcacgtg ttttcaatgc
     1561 gttatctgtc gatgtaagca ccagggccgc ggcgccatcg gaaacgaggg aacagtcgtg
     1621 aagacgcagc ggatcggcga tcatcggatt gcttcccttg ccttccgcgg gcagagcaag
     1681 aatcgcctcg gcggtaaaaa gacccgcccg ctccgccacg ccccccggac caaaatgcgc
     1741 caacggattc tcagctccat ttttgtagca taaagccgct accgtcgcga acatccggcg
     1801 caattcgtcg tcggctaaac cgtaacgctt ttgataccct ttggcataag cggcaaacag
     1861 catgggaaag gacatgcctt gggaaccttc cgtgggccaa tgggaagaac atgacaaggc
     1921 atgcgtcatc tctctggtgc cgagaaggtt catcttctcc acgccgagca cgagaacatg
     1981 acgatagcgg ttggcttcta gggaatagag ggcatcgtat agtgcagcgg aagaagaagc
     2041 gcaggcgcat tccgtacgag tcatcggttt gaaacgcaaa tgggggtcga tctcggccgc
     2101 cagaggtgct acgtgctcct ggttgacaaa aagggaaggg gagcaggagc caacccatac
     2161 ggcatcgatc tgatcggccg tcaggcccgc atccgcgatg gcgcctcgtc ctgcctccgt
     2221 gatcaaatca taaaatgata ggctatcggt gacttgtttg gtttctttgt ctcgctgaac
     2281 gaaggcacca aacttggtgt tgtaggcgcc tatgatactc acacgactca tggggacctc
     2341 cagcgctacg tcatagcgca ttcgataagg gaaaacgatg ctcgatgact aagcaagagt
     2401 cgagccacga tgggcatagt cgatactaat ttcgttgccg tcgagggtaa cgtcatggaa
     2461 agatggctca gaaccagcct agacgacgaa tttccctcgc ttgcgtgaag tcttttcccg
     2521 gatcatcggt gacaccgtcg atgaaaagca tcgatgtggc gcgggccact tctccaaagc
     2581 actcgaatac gaaagatact tgccgttggg aatagccttg ttggctaccg gctttgggtt
     2641 tggccgttac cttcgtggaa acacgcaatg cttgcgtgaa ggtgaaatcc ggaaggcgta
     2701 gttctcccga cgcgtccaca ctgacctcat acacatcttc ctgggaccag ggtgataatc
     2761 cacgcagcgt gccgtctttg acgatacccg tttgtgtcca cgccttgccc accatcaaag
     2821 gaaaagggaa aaaatcgatg ggtttttcat agaccaatag cgttttcccc tcgggaggat
     2881 tctccaacac cgaggcaacg ccgtgcaggc gcaaggcttg ctcatcatgg gaatagatac
     2941 ctaccaaact tccacttaga tccgagggca gggcaaagtg agcccccacg aaagcgttgg
     3001 cataccattg cgtggtaagt ggttcagcca cgattttggc gacgagatgg gtcgtatcgt
     3061 cggccgacca attccaaatc cgcttgcctt ggtcgttcac cacgccttcc gtatttacgt
     3121 acgtcccttg ggtggcggtg gctggcaagg gcggagtgac gatataggat gcgacttgtt
     3181 gaagcttcgg tgccatctcc caggattcga cgacgccgtc gaggttgggt atgcattgga
     3241 gtggttcgga tacgcctccg tcgtatgatt cgtgacgaat cggcatggtg tcgttgtccg
     3301 cggcgcaagc gctgagaacg atggcaaaca gaccgaaaac agggatgaat cgtcgcatgg
     3361 cttagaatcc aaaggaaagg cggagccgat atagttgggc tggtttggcg gtgaggccga
     3421 ggtcccggtt gttcaaaccg gcgcccggga aaaggacggc atgctcgaaa gcagcggcaa
     3481 aaccatcgtc actcgtgtac acgagcgtgg ggttgagttc gaatccaagg tatttttcgc
     3541 ctccgggcgt agacgaagcg tagttggcaa acgatgcgat gccggcaaca gacgcctgca
     3601 agcgcgcggt atcgaagtcc aacaatcgaa ttcgagcgtg ggggcgaaga tagacggcat
     3661 ccgtaaccgt gccgataatc tctcggaata atattcgatc gatgcgataa tccgggtgaa
     3721 atcggaagtt gtcgacacga aagtcataag gcggaagccc ttgggggcca tcgaggtcgc
     3781 caggttgagg cgccgtcagc ttcgcaagtg gcggtcgggc accgaatccg ggggcgctat
     3841 ccccgcttgc gtatcccatg tcaaacccgc ctcccgccag accgttgggt tgtccaatct
     3901 cactctccaa ggctccgccg aattgacgcg ccttgacctt atgattcatc tcgagccccg
     3961 gaatcaacga cgcctgatcg aagttcgcgg tcgaataagc cccttcgact tcgacccgaa
     4021 aaccgtatac cgtaaaacgc gaccaaaggc tgaaaacatt ggccgtcaaa ccacgcccca
     4081 tcacctgtcc cgcgtcgatg gcggtggggc ccgcgacaat gggcaaatac gtggccggaa
     4141 tatccttgtc ttgccagcgt cgcgcataca atccaccgta ctcgaacgtg gcctttcctg
     4201 ctttggccct gcgacgtcgc gcggcgtcat cgcgccaatt aagcagagct accgacatgg
     4261 aacggacggc ggccgaaggt tcgatatcga tggagcggac ctggtctccc cgtccgacga
     4321 tatgcccaga ggcggaaaag tcgtaggcga gcgccaaaat atgggaggct gcggcggtgg
     4381 cgaaggtgat ccggtccgcc gcatcgccgc tatcacaatc agcgcaatct ccgctattgc
     4441 caagcattcc caaaccccaa tggtgcccca ttcgcccggc agctaggtag ccgaggggag
     4501 tgacgacctc gccccacgcg cgcttgatgc gaaacgccgc ggagggaggt tgttgtgtca
     4561 ccgtgccccc ggggatgcca tccgcactgc ctcctaaagg catattatcg aggatatcga
     4621 cacgggcttt gacagccacg actccgccgg gagcgtaggc ggcaaaatcc gtgcgaagcc
     4681 gcacatcgtg gtacgttagc cattgcgcct tggggtcgct gaccgggact gggaaaaaca
     4741 cttgtccaga aggggttggt ccccggtcca ggtccaggtt gtacaacgct tcggaacgaa
     4801 cgcgaaacgc ccctccgagc acgagacgag tttcggtggt gggaacgata tcttggccgt
     4861 ggtcggtgaa tcccgtggcg aaagcgcccg aagccacgca aaacgcagcc aaagcagccg
     4921 aaatcggtaa aaaagagcgg ctcacgacgg ttgctcctca gccaggaaac gaagtagttc
     4981 ttgtgtcgag gctcccggca cttcgagcat cggcaggtgg ccgcatcgcg gatataccac
     5041 catccgcgct ctgggaagct gctgcaacaa tcgctcgcca tacgtgagcg gtgtgaccgc
     5101 atcttcgcga ccccacatca aaagcaccgg ttgatggacc gtgggataac gtttttgcag
     5161 ctcggaataa cgcatggcgc gcactcccgc gagcgccgcg gccaaggttc caggtcgatt
     5221 caacaaccct tggacatcct ccaccacctg ctcggtaatg tgtttttcgg gctcgtaata
     5281 ggcaaaagac agcttttcat cctgccggtc cggataaaaa acaccataga tgatctcccc
     5341 caatacgggt gtttgcgccc atttgaatag cgtgggcttc tgctcttcga aatacatccc
     5401 attgtacaag gccacgcgct tgacccgcgt gggcgccgca atggcgaccg ccagggaaac
     5461 cgaggagccc caagaatggc cgaccaccgt aaaatccttg actccccgct ggtctgccaa
     5521 cgccacgacg cgccgggcct gggcaagaat cga
//
LOCUS       DAOSOR010000401         5525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14849_length_5525_cov_4.206947, whole genome shotgun sequence.
ACCESSION   DAOSOR010000401 DAOSOR010000000
VERSION     DAOSOR010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14849_length_5525_cov_4.206947"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1920
                     /locus_tag="PK710_18195"
     CDS             <1..1920
                     /locus_tag="PK710_18195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11715.1"
                     /translation="PSKPGCETNTTNNPEHCGFCNNACKFANGTGKCVNSTCQLDACD
                     AGWGNCDGNASNGCETNTNTAVNHCGDCGNTCPSRPHAKATCNNGSCGFVCDPGWENC
                     DGSATNGCEVNTQSSVNHCGACGKACNLPHATAACSNGSCTISSCDSGWGDCNGNPTD
                     GCEVNLNTSPNYCGSCGNKCSVTNGTAGCSNGKCTIASCNAGYVDCDGAVTTGCEINT
                     TNDVFNCGSCGVACNSTNGTAMCQSSKCVIACYPGYGNCNGLVEDGCETNTQSSPNHC
                     GGCGQTCSNNHISNPTCTNGVCSGACTTGWADCDSNKLTNGCETNTNTSVDNCGGCGN
                     ACSGNNIPSRSCANGVCNGSCASGYADCNGNKLTDGCETNTASDVSHCGACNKPCSTN
                     HVSPLCSNGQCIGKCQIGYSDCNNNKQTDGCEVNTAADILNCGTCGNSCTNPTPPQVS
                     TVGCLSGSCTVTKCDDTYYDQNQTFGDGCECQADTIANSCSSASTISTLYTATITMPS
                     TSTYYNIVPTGDEDWFTGAIYDYGIYYSGCNYRPKIELIDPSNLLLMNVYNASSCSSS
                     TGYACSTAEGGTSTKGVRTWEFGHSATCGDYQAIDPTPATGTYMQQSTSYRIRVYANA
                     SSSSCLPYQIRLSRY"
     gene            complement(1928..2800)
                     /locus_tag="PK710_18200"
     CDS             complement(1928..2800)
                     /locus_tag="PK710_18200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11716.1"
                     /translation="MPQSRWPHVLWTPLVVAMAVGVVDEAKADDKQVCLESYDKSQTL
                     RQEGKLVAAREQMLSCARNVCPEILRRDCTQWLSEVDETIPTVVLSVKDKRGQDLVDV
                     RVTVDGEALVTQIDGKAVAVDPGVRLFRFDAPGEEPVEKRIVIHAASKNRGITIQFGS
                     GSVDVEPQPDVTKPQQESGGPGVLPYVFGGIGLVGVGAFAFFGTQFDSKLDDLDKCKP
                     NCSKADTDDASTTRTLAWISGGVGVVSLGVATYLFLSGSSDTPASVEVASALPRVELV
                     PVRGGALGTFGTRF"
     gene            complement(2821..4641)
                     /locus_tag="PK710_18205"
     CDS             complement(2821..4641)
                     /locus_tag="PK710_18205"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT11717.1"
                     /translation="MSQSGTEYPRQVGRYWLFDEIASGGMATVRYGRLVGERGFSRTV
                     AVKCLHAHFAKDEEFSKMFLDEARLAARIQHPNVVSILDVVGRDGELYLVMEYVQGES
                     LSKLIRAARRQKTRIPLRIVASIIGGMLDGLHAAHEATSELGVPLGIVHRDVSPQNVI
                     IGTDGVSRVLDFGVAKAAGRMQSTRDGQLKGKLAYMAPEQLKSEIVDRRTDVYAASVV
                     LWEALTGRRLFKADDEIGIFGMVLQNKVEPPSSIIPSIPKGYDAVTMRGLQLEPDQRF
                     ATAREMAIALEKVAGVASPREVGAWVEELAHETLHQRTSKISELESKSASDLQKMVPQ
                     NVAVNLSVGAMPLGSVSRIAPPPPGRGLPPPRLPPPRLREGSNPQIVPSFPVAISEVP
                     PADEDGTLATLISDTTASSLELSVPSAVISDSSDLVSSPSQLTSLSMAQERPKTGPKP
                     RVLVAVVGSALLLSLLVVAGLALLTDQFGTRVDHDEDAGSVAALGEPQKNEKEAGLQE
                     QADASTPTAPAVDAAVDASVHDVAVDALASATSKKVSVSTTRPVGVPVPVDTKPPVVP
                     PTQTTRPAAVDTTSKPPKNDCNPPYTIDARGVRVPKLNCL"
     gene            4952..>5525
                     /locus_tag="PK710_18210"
     CDS             4952..>5525
                     /locus_tag="PK710_18210"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOT11718.1"
                     /translation="MAEQSLWKVLLRRHSIKLLASLVIGVAFWLILRGGGLPVLPESS
                     SLDGIRLWTWPAYACSVALWSFIRAARTRHLLRLVAEVPLRQMLSVSWIGFLSIFVMP
                     FRLGEIVRPALFHQTGRVSFAASTGACGAERIIDGLLLMTVLGVSLPLGGPLDPLPNH
                     IGKLPIPVVAVPAAAYSTLLVFVLAFIAMAV"
BASE COUNT         1258 a         1647 c         1438 g         1182 t
ORIGIN      
        1 ccaagcaagc caggctgtga aaccaacacc acgaacaatc ctgagcactg cggtttctgc
       61 aacaacgcat gcaaattcgc caacggcact ggcaagtgcg tcaatagcac gtgtcaactc
      121 gatgcttgtg atgcgggctg gggaaactgc gatggcaatg ccagcaatgg atgcgaaacg
      181 aataccaaca cggcggtgaa ccattgcggc gactgcggca atacctgccc gtctcgtcct
      241 cacgccaaag caacctgcaa caacggctcc tgtggtttcg tttgcgatcc gggatgggaa
      301 aactgtgacg gttccgccac caacggttgt gaggtcaata cgcaatcgag tgtcaaccac
      361 tgtggcgcat gtggaaaggc atgcaatctt ccccatgcca ctgcagcttg ctccaatggt
      421 tcatgcacca tcagctcttg tgatagtgga tggggggact gcaacggaaa tccgacagat
      481 ggatgcgaag tcaacctcaa caccagcccc aactactgtg gttcctgtgg aaataaatgt
      541 tccgtgacga atgggacggc cggttgttcc aatggcaaat gcacgattgc atcgtgtaac
      601 gcgggttatg tcgactgcga tggggccgtc acgactggat gtgaaatcaa caccacgaat
      661 gacgttttca actgcggaag ttgcggcgtt gcttgcaata gcaccaacgg taccgcgatg
      721 tgtcaatcca gcaaatgcgt catcgcctgt tatcccgggt atggaaactg caacggcctg
      781 gtcgaggacg gttgcgaaac caacacacaa tccagcccaa atcattgtgg tggatgtggg
      841 cagacgtgca gcaacaacca tatctcaaat ccaacttgca ccaacggtgt ctgctcagga
      901 gcctgtacca cgggctgggc ggattgcgac agcaacaaac tgacgaatgg ctgtgaaacc
      961 aacacgaaca ccagtgttga taactgcggc ggatgtggca atgcatgcag tggtaacaac
     1021 ataccctccc gaagctgcgc caatggagtc tgcaacggtt cctgcgcctc cggctatgcg
     1081 gactgtaacg gcaacaagct cacggacgga tgcgaaacca atactgcatc cgatgtgtcc
     1141 cattgcgggg catgcaacaa gccctgctca accaaccatg tatccccctt gtgttccaac
     1201 ggccaatgta ttggcaagtg ccagatcggc tattcagact gcaacaacaa caaacaaacg
     1261 gatggttgtg aggtcaatac cgctgccgac atcttgaact gtgggacctg cggcaatagc
     1321 tgcaccaatc caaccccacc ccaagtctcc acggtcggat gcctttcggg gagttgcacc
     1381 gtcacgaaat gcgatgacac ctactacgat caaaaccaaa cgttcggtga tggttgtgaa
     1441 tgccaggccg acacgatcgc gaactcgtgt tcatccgcca gcacgatcag cactctgtac
     1501 acagcaacca tcacgatgcc ttctacgtcg acctattaca acatcgttcc cacaggagac
     1561 gaagactggt tcacaggtgc gatctatgac tacgggatct actattcggg ttgcaattat
     1621 cggccgaaga tcgaactcat cgaccctagc aatctgctgt tgatgaacgt gtacaatgca
     1681 agctcttgtt catcatcgac gggctacgcc tgcagtaccg cggaaggcgg cacctcaacc
     1741 aagggagttc gcacgtggga gttcggtcat tcggcaacct gtggagacta tcaagccatc
     1801 gatcccacgc ccgccacggg aacctatatg cagcagtcta cttcgtaccg aatcagggtg
     1861 tacgccaacg cttcgagttc gagttgcctg ccgtatcaga tcaggctgag ccgctactga
     1921 ttttccgtca gaatctagtc ccaaacgttc ccaacgcccc gccacgaact ggaacgagtt
     1981 cgacacgagg cagcgcggaa gcgacttcga cactagcagg cgtatccgac gaaccggaca
     2041 agaacaaata cgtggccact cccaaggaca caacgccaac cccgccagaa atccacgcta
     2101 gggtgcgtgt ggtcgatgca tcgtcggtat cggctttgga acaattgggc ttgcacttgt
     2161 ccaagtcatc gagcttgcta tcgaactgcg tgccgaagaa cgcgaaagcg cctacaccca
     2221 ccaaaccgat tcctccaaac acgtagggta gtactcccgg ccccccactc tcttgctgcg
     2281 gcttggtgac atcaggctgc ggctcgacgt ccacagaacc actaccgaat tggatggtga
     2341 tcccgcggtt tttcgaagca gcatgaatga caatccgttt ttccacaggc tcctcgccag
     2401 gggcatcgaa tcgaaacaga cgcacccccg gatccacggc caccgcctta ccatcaatct
     2461 gcgtgaccaa tgcctcacca tcgacggtca cccgaacgtc cacgagatct tgccctcgtt
     2521 tgtccttcac cgacagcacg accgtcggaa tggtttcatc cacttcgctc agccactggg
     2581 tgcaatcccg acgaaggatc tccgggcata cgttgcgagc acaagacagc atctgttccc
     2641 tggcggccac gagtttgcct tcttggcgaa gggtttgaga cttgtcgtag gactccaaac
     2701 aaacttgttt gtcatccgcc ttcgcttcgt cgacaacacc aacagccatg gcaacgacaa
     2761 ggggagtcca taaaacatgg ggccatcgag attggggcat acgttccttc ctccggaacc
     2821 tcacaaacag ttgagtttgg gcacccgtac accacgggca tcgatggtgt agggtggatt
     2881 acagtcattc ttcggtggtt tggatgtggt gtctacagcg gctggccgcg tagtctgcgt
     2941 aggcggaaca accgggggct tggtatccac ggggacgggc acaccgaccg gtcttgtcgt
     3001 tgaaacggag acttttttcg acgtcgcgga agcaagtgcg tcgacagcaa catcatgcac
     3061 cgatgcatcc actgccgcat ccactgccgg cgcagtgggc gtcgacgcat ccgcttgctc
     3121 ctggagtccc gcctcttttt cgtttttttg tggctctccc aaagcagcca cggaacctgc
     3181 atcctcatcg tgatcgactc gtgtaccgaa ttgatccgtc agcagcgcaa gccctgccac
     3241 gacgagcaaa gacagcaaca gggcgcttcc gaccacggcc accagcacgc gcggtttggg
     3301 tcctgttttg ggtcgctcct gcgccatgga taggctggtg agttgtgaag gtgaacttac
     3361 gagatccgag gaatctgaaa tcactgccga cggtacagac agctccagag aactggccgt
     3421 cgtatcagaa atgagcgtgg ccaaagtacc gtcctcatca gcaggaggca cctcggagat
     3481 ggcgacagga aatgacggaa cgatttgggg attcgaacct tcgcgcagtc gaggaggggg
     3541 aagtcgaggg ggaggtagtc cgcggccagg agggggcgga gcaattcgag acacgcttcc
     3601 cagcggcata gcgccgacgg aaagattgac cgccacgttt tgaggcacca tcttttgcag
     3661 atcggacgcc gacttgctct cgagttccga aatcttactc gttcgctgat gcaacgtctc
     3721 gtgagccagc tcttcgaccc atgcccccac ttcccgtgga ctcgccaccc cagccacttt
     3781 ttccaatgcg atcgccatct cccttgcggt ggcaaagcgc tgatcgggct ccaattgcaa
     3841 tcccctcatc gtgaccgcat cgtaaccttt cggaatgctc ggtatgatcg aactcggcgg
     3901 ctctacctta ttttgcagca ccatcccgaa aataccgatc tcatcgtcgg ctttgaataa
     3961 ccgcctgccc gtcaacgctt cccaaagcac cacactcgcc gcatacacgt ccgtgcgacg
     4021 atcgacgatt tcggacttga gctgttcggg cgccatatag gcgagctttc ccttgagctg
     4081 accatctcgg gtggactgca ttcttcccgc cgccttcgcc acaccgaagt cgagcacacg
     4141 ggaaacccca tcggtcccaa taatcacgtt ctgaggagaa acgtccctgt gaacgatacc
     4201 aagcggaaca ccaagctcgg atgtcgcttc atgagccgcg tgaagtccat cgagcattcc
     4261 gccaatgatc gacgccacga tccgcaacgg gatccgggtt ttctgcctgc gggcggcccg
     4321 aatgagcttc gacaaagact cgccctgcac gtattccatg acgagatata actcgccatc
     4381 tcgccccacc acatcgagaa tcgaaacgac attcggatgc tgaattcgag ctgcaagcct
     4441 tgcctcgtcg agaaacattt ttgaaaattc ctcgtctttg gcgaaatggg catgcagaca
     4501 cttcacagct accgttcgcg agaagcctct ttccccgacc agccgcccat accgcacggt
     4561 ggccataccg ccagaagcta tttcatcgaa cagccaatag cggccgacct gacgcgggta
     4621 ttctgttccg gactgggaca ccatggtagt ttggcaaatc taccgaccga acggcaaaga
     4681 atcgacttcg gaagtatagc gcactcgttg cgaatgagga tcaaaggctt gtatgggagc
     4741 cctttgcaac agtatttttc caaaagattc gtgattcatt gcctcaggac cgctgtttat
     4801 tcccaaagat cataccgcaa aatccctttt cggctttcca gcaacagggc cttttgaccc
     4861 aaaccatgat atttgatcgg ccatagcccg aatctggcgc taccgcggtc ccgttgctag
     4921 attcggctgc cgcttgccca cggcatccct catggcagaa caatctctct ggaaagtcct
     4981 ccttcgacga cattccatca agcttttggc atcgctcgtc attggtgtgg ccttttggct
     5041 gatattacgg gggggaggcc tgcccgtgct gccggaaagc agcagcctcg atggtattcg
     5101 cttgtggact tggcccgcgt atgcctgcag cgttgcgctg tggagtttca tacgcgctgc
     5161 ccgaactcgc cacttgttgc gtctcgtggc cgaggttccc ctgcgtcaaa tgctgtccgt
     5221 atcgtggatc ggctttctgt cgattttcgt catgccgttt cgcttgggag aaatcgttcg
     5281 acccgcattg tttcatcaaa cgggccgagt gtcttttgcg gcctcaaccg gagcgtgtgg
     5341 cgcggaacgc atcatcgacg gccttttgct catgaccgta cttggcgttt cgttgccgtt
     5401 gggcggcccg ctcgatcccc ttcctaacca tatcggaaaa cttcccattc cggtggtcgc
     5461 cgtacccgcc gcggcctact cgacactgtt ggtcttcgtc ttggctttca tcgccatggc
     5521 agtat
//
LOCUS       DAOSOR010000402         5506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14907_length_5506_cov_4.319758, whole genome shotgun sequence.
ACCESSION   DAOSOR010000402 DAOSOR010000000
VERSION     DAOSOR010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14907_length_5506_cov_4.319758"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PK710_18215"
     CDS             complement(<1..473)
                     /locus_tag="PK710_18215"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HOT11719.1"
                     /translation="MGELYSGLSWVSKARKGLDMDVLEAIKGRRTIRKFKKDPIAQEQ
                     LDEIFEAAMWAPSHGNTQPWEFVVVGPKARARLLAMVQTKVDELLAAPDVPEPKRKGL
                     LSLRDDFGGAPYMVAVVSRPGVEPLEIVENPECTSAAVQNMCLAAWARGIGAIWLS"
     gene            979..1785
                     /locus_tag="PK710_18220"
     CDS             979..1785
                     /locus_tag="PK710_18220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11720.1"
                     /translation="MKRIPTLLLTSVMAMVALGCGASRSMGDNSPPIPKESASAPALQ
                     RSLFSRDLTGSVSEHDIQTILGSRVELGFPARMGVVVLGEPFRAESAASIDQHSMIGP
                     AMVRALDASPYVSHITDISTGLPNPQGIEGLRTIAARYRVRYLLVCHVAAEDGSHLNN
                     WAWLYPTVLGFFMAPGVTVGSHGHVEASLLDVKTGTVQFTTMEPFESSSVTWVIGSSR
                     HHEQQDAKALQNALDALARSVLMQMERVAKWEKNQEKSPAHTEPEDLALR"
     gene            2235..4328
                     /gene="ligA"
                     /locus_tag="PK710_18225"
     CDS             2235..4328
                     /gene="ligA"
                     /locus_tag="PK710_18225"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOT11721.1"
                     /translation="MSHPKQLLLDEYEALASQIAYHERLYRDGVPEIPDAAFDDLVDR
                     YQQLADELEIPPEQRIDQTPGVDHTDGFVQVEHRSPMLSLEKISPYRRDGKGDSLPSL
                     GQFLSWYDRRQKDLGIHRHEQLPLLVEPKIDGISVSLLYVRGQLQRAVTRGDGKKGDD
                     ITRQVKSARAVPERLDGVRGSLEVRGELYWPLDAFSAHNQKLILFGEKPLANPRNGCA
                     GLMKRKDPEGLHHAGIRSFLYQIPWSEDLPLPTSQFEVLQWLKHCGAPVYLDEIFLAH
                     HPHDAFHYCEQYAERRPHLPYEIDGMVIKLDRLDLYSRLEGTGHHPHWAIAYKFPPER
                     KPTLLKGITVQVGKSGKLTPVAELEPVLVAGTTVSRASLHNFPELERKDVRVGDTVFI
                     EKAGEIIPQVVSVDLDKRPINTESYARPTACPACGADVVSEDIFIYCPNPSCPHQVRE
                     RLRHFASRGAMDIEGLGAALVEQVIDKLGVVSPDDVFRMKEADLAGLERMGKKSAANL
                     MKSLQIAKDRGLARVLVGLSIRHLGTTMAEDLANHFESVDALLDFAQRYRQGDPQAIE
                     QVAPEKGSGVIEGMARKTADSIFAELASPSVRGVIEGLRQVGVLLEAKRQLSDAVVGV
                     VGKSFVLTGSLPTLKRDEASALIKAAGGIVGTSVSKKTDYVVVGQDAGSKLDKAQKLG
                     INLLDEAGLLALLRK"
     gene            complement(4362..4802)
                     /locus_tag="PK710_18230"
     CDS             complement(4362..4802)
                     /locus_tag="PK710_18230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11722.1"
                     /translation="MRFRWLYRMCLLVIGAVPCSGCSKSSTESAVSESNAQITQIVFL
                     DQETICPIIKKGIDSTWQELQAATKGRDIRVMRVYRDTQGSLARKYTLMKPTAIMPGM
                     YFLTSEEELVDFLQGEKKEEQIKAVLDAARPAHPEPSASGQEVK"
     gene            complement(4863..>5506)
                     /locus_tag="PK710_18235"
     CDS             complement(4863..>5506)
                     /locus_tag="PK710_18235"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOT11723.1"
                     /translation="LGAVARGIDRGLASAEVARKLVADVGMVADIMSGSGSQEAASRR
                     RANLEHFFNTLARFDKTGETGAKALSEYLQLLTLKTDADDDAGNVVTLTTMHGAKGLE
                     FHTVFVVGVEEGLIPHARTLDGKTTDVTPQDVEEERRLFYVALTRARTRLYLCRAKQR
                     TFHGKGSKRAPSRFLKNIPEDRLERVDIKEQAPPSRESMLEGAEALLAALEKG"
BASE COUNT         1198 a         1849 c         1314 g         1145 t
ORIGIN      
        1 gaaagccata tcgccccaat cccgcgggcc caggccgcca gacacatatt ctgtaccgct
       61 gccgatgtgc attctgggtt ttctacgatt tccaatggtt cgacccccgg acgactcacc
      121 accgcgacca tatacggcgc gccaccaaag tcgtcgcgaa gagatagaag ccccttgcgc
      181 ttgggctccg gaacatctgg cgctgccaga agctcatcga ccttggtttg caccatcgcc
      241 agcaatcgtg cccgcgcctt cggtcccacc accacgaatt cccagggctg cgtatttcca
      301 tgagatggcg cccacatcgc cgcctcgaaa atctcatcga gctgctcttg cgcaatggga
      361 tccttcttga acttgcgaat cgtcctgcgt cccttgatcg cttccaatac gtccatgtcc
      421 aaccctttcc tcgctttcga tacccatgac aagccactat atagctcgcc catgagcacc
      481 aaccaccgtt agaatccgtg caagaacgcc aaccttggca caactgcccg cggggatcca
      541 gagagtcttt gccaaccggt catggtcaag gcaaacgcga aaacgctcac ggcccacggc
      601 ccacgaaacc ggtcacggtc acagcccacg aaatcggcca cagtcaaccc atattgatcc
      661 cagcagtgcg atgaggcgca tgaccaaatc catcaaagca aacccgtccg ggaggcggga
      721 gactatactc gacgtatcgc aaaatgccgt agcgtcgatg ccatctgggg atggggccat
      781 gacaagcggt ttgcctacat cccctggctt tcgaatcact tccccctgct catcaatccc
      841 gttgccccaa caggctgtcg tcgataaaaa caagatcttt catccgctca ttacattgtt
      901 ttatctattg tttacgcgta gttatctatt tttgatccac cgctgtaagg tatccgcttg
      961 gcaccacgtt ccagagccat gaaacggatt cctaccttat tgctcacttc ggtcatggct
     1021 atggttgctt tgggttgcgg cgcttccaga agcatggggg acaattcccc gcccatcccc
     1081 aaagagtctg cctccgcccc tgcattacaa cgttcgctat tttctcgtga cctgacgggt
     1141 tccgtcagcg agcatgatat ccagacgatc ctcggttccc gcgtggaatt gggcttcccg
     1201 gcccgcatgg gtgtcgtggt tctcggtgag cctttccggg ccgaatccgc tgcttccatc
     1261 gaccagcatt cgatgattgg ccccgccatg gttcgtgccc tcgatgcttc cccttacgtt
     1321 tcccatatta ccgatatatc caccggcctg cccaatcccc agggaatcga aggcttacgc
     1381 accatcgccg cccgctaccg cgttcgatat ctgctcgttt gccatgtagc cgccgaggat
     1441 ggctcccact tgaacaattg ggcatggctc tatcccaccg tgctcggctt cttcatggcc
     1501 cctggcgtca ccgtgggctc ccacggacac gtcgaagcca gcctcctgga cgtcaaaacc
     1561 ggtaccgtcc aatttaccac catggagccg ttcgagtcgt cgagtgtcac ttgggtcatc
     1621 ggttcttccc gacaccacga acaacaagat gccaaagctt tgcaaaatgc gttggatgcc
     1681 ctggctcgct cggtattgat gcaaatggaa cgggttgcca agtgggagaa aaatcaagaa
     1741 aaatccccgg cccataccga gcccgaagac ctggccttgc gctgacgggc tatcgcaaaa
     1801 gcgcaaacgg cgaattcacc tcgacgaccg aaccctctcg tttcgtatcc cctttgttat
     1861 ccgagtaccg accgggcgct gaatccccct tctcctgagc catcccctca tttttccccg
     1921 gggcctttcc ttcatcctac gacagatgga aacaagttcc taccatcgct tggccacggt
     1981 cacggtcacg gtcacggtca cggcatacgc gaaacgctca cgatcatccc atattgttca
     2041 ccgcagtgcg ccagtcccat ggttagtagc tcagggaaac tgttgggggt gctccaaggc
     2101 aagctatgga atcgaggtag gataagaggc gatacgccgt atccttcaga acatttttac
     2161 cccacctccc caacgccacc acccccgcca acagcctgga ctcctttccc aacttatcca
     2221 acactcctcg gcccatgagc catcctaagc agctccttct cgacgaatac gaagccctcg
     2281 cctcccaaat cgcctaccac gaacgattgt atcgcgatgg cgtccccgaa attcccgatg
     2341 ccgccttcga cgatctcgtc gaccgctacc aacaactcgc cgacgaactc gaaatccccc
     2401 cggagcagcg catcgatcaa acgcctggcg tggaccatac cgatggattc gtgcaggtcg
     2461 aacatcgctc gcccatgctc tcgctcgaaa aaatctcccc ctatcgtcgc gacggcaaag
     2521 gcgattccct gccttccctc ggacaattcc tctcctggta tgaccgaagg caaaaagacc
     2581 tgggcatcca tcggcacgag caactccccc tgctcgtcga acccaaaatc gatggcatct
     2641 ccgtctcgct gctttacgtg cggggccaac tccaaagggc ggtcacgcgc ggggatggga
     2701 aaaaaggcga cgatatcacc cgtcaagtca aaagcgcccg cgccgtaccc gaacgactcg
     2761 atggcgtgcg tggctcgctc gaagtgcgcg gcgaactata ttggcccctc gacgcctttt
     2821 cggcccacaa ccagaaactg attctcttcg gcgaaaaacc cctcgccaac ccccgcaatg
     2881 gctgcgccgg attgatgaaa cgaaaagacc ccgaagggct gcaccacgcc ggtatccgct
     2941 cgttccttta ccaaatcccc tggtccgaag acctgccctt gcccacgtcg caattcgagg
     3001 tcctgcaatg gctaaagcac tgcggtgctc ccgtgtacct cgacgaaatc ttcctcgccc
     3061 atcatcccca cgacgccttc cattactgcg agcaatacgc ggagcgtcgt ccacatctgc
     3121 cgtacgaaat cgatggcatg gtcatcaaat tggatcgcct cgacttgtac tcgcggctcg
     3181 aaggcaccgg tcaccacccc cattgggcca tcgcctacaa attccctccg gagcgaaaac
     3241 caaccctgct caaaggcatc accgtgcaag tcggaaaatc cggcaaactc acgcccgtcg
     3301 ccgaactcga acccgtgctc gtcgctggca ccaccgtctc ccgcgcttcc cttcacaact
     3361 tccccgaact cgaacgcaag gatgtgcgcg tgggcgatac cgtattcatc gagaaagcgg
     3421 gcgaaatcat tcctcaggtc gtctcggtgg atcttgataa aagaccaata aataccgaat
     3481 cttatgccag gcccaccgcc tgtcccgcct gtggcgccga cgtcgtctcc gaagatatct
     3541 tcatctactg ccccaacccc tcctgccccc accaggtgcg tgaacggctt cgacacttcg
     3601 ccagccgcgg cgccatggat atcgaagggc tcggtgccgc cctcgtcgaa caagtcatcg
     3661 acaaacttgg cgttgtctcc cccgatgacg tgtttcgtat gaaagaagcc gacctggccg
     3721 gtctcgaacg catgggaaaa aagagcgctg ccaacctcat gaaatccctg cagatcgcaa
     3781 aagatagggg actggcgcgc gtgctcgtcg gcctttctat tcgtcacctc ggaaccacca
     3841 tggccgaaga cctcgccaat cacttcgaaa gcgtcgatgc cttgctcgac ttcgcccaac
     3901 gctaccggca aggcgaccca caagccatcg agcaagtcgc cccggaaaaa ggcagcggtg
     3961 tcatcgaagg gatggcacga aaaaccgccg atagcatctt cgccgagctg gcttcccctt
     4021 ccgtgcgtgg cgtgatcgag ggccttcgcc aggtcggcgt tctgctcgaa gccaaacgcc
     4081 aactatcgga tgccgtagtc ggcgtggtcg gcaaatcctt cgtcctcacc ggcagcttac
     4141 ctaccttgaa acgcgacgaa gcaagcgcgt tgatcaaagc ggcggggggg atcgtcggaa
     4201 cttccgtatc caaaaaaacg gattatgtcg tggtcggcca ggatgcgggc agcaagctcg
     4261 acaaggccca aaaactcggg attaaccttc tcgacgaagc aggattgctt gctttgttga
     4321 ggaaataatc ttgcgcgtca tcagcatcgc acgagcaggc gtcacttcac ctcttgcccc
     4381 gaggctgaag gctccggatg cgctgggcgc gcggcatcca acaccgcctt gatttgctct
     4441 tctttctttt ccccttgcag aaaatcaacc agctcttcct ccgacgtcag aaagtacatg
     4501 ccgggcatga tcgccgtggg cttcatcaac gtgtacttgc gcgccaaact gccttgcgtg
     4561 tcccggtaca cgcgcatcac ccggatatcc cttcccttgg tggcagcttg aagctcctgc
     4621 caggtagagt cgatcccctt tttaataata ggacagatcg tctcctgatc caaaaacacg
     4681 atttgcgtga tttgcgcgtt cgattcggat accgcggatt ccgtcgagga tttggagcat
     4741 ccactgcacg gcaccgcccc tatcacgagc aaacacatcc gatacaacca acgaaaacgc
     4801 atgagtccaa ccttcgggta cgtcgatgag gatacaacgc gagacgcgcg gagcaagcct
     4861 gctcatccct tctctaatgc cgccaacagt gcttcggccc cctccaacat cgattcccga
     4921 ctcggaggcg cctgctcctt gatatcgacc cgttccaatc gatcctcggg aatatttttc
     4981 aaaaatcgac tcggcgctct tttcgaccct ttgccatgaa acgtccgctg tttcgcacgg
     5041 caaagataga gtctcgtgcg cgctcgcgtc agcgccacat aaaataaccg cctctcctct
     5101 tccacatcct gaggcgtcac atccgtggtt ttcccatcga gcgttctggc atgggggatg
     5161 agcccctctt cgaccccaac cacaaaaacc gtatgaaatt caaggccttt ggctccgtgc
     5221 atcgtcgtca gggtcacgac gttgcccgcg tcgtcgtccg catccgtttt caatgtcaat
     5281 agctgcaaat actcagacag cgctttggca ccagtctccc cggttttatc gaaacgcgcc
     5341 agcgtattga aaaaatgctc caggttggct cgccgccgcg aggctgcctc ctgcgatccc
     5401 gagccggaca tgatatcggc aaccattccc acgtccgcca ccaacttgcg cgccacctcc
     5461 gccgaagcca gccccctgtc gatcccacgg gccaccgcgc ccaatg
//
LOCUS       DAOSOR010000403         5504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_14919_length_5504_cov_3.806019, whole genome shotgun sequence.
ACCESSION   DAOSOR010000403 DAOSOR010000000
VERSION     DAOSOR010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14919_length_5504_cov_3.806019"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="PK710_18240"
     CDS             <1..778
                     /locus_tag="PK710_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HOT11724.1"
                     /translation="VAHRGYEDFANYRYLSQRDDSLLLASRKRRVTDTTIPEDQAFRA
                     VEARIVPMWKHASEVGVRRFWHEYFYDLVQALELSVYEGDPEACWLLEAARRRLPIVV
                     PGYEDSTYGNIFASYVKSGECNANIVKSGIEYMVAFYEQYQALSAGPGVGFFQIGGGI
                     AGDFPICVVPSLKHDVGQSAKPWAYFCQISDSTTSYGSYSGATPNEKITWDKLTEDTP
                     MFVIESDATLVAPLVLRALLESKRDPAGAEALIARKEAEA"
     gene            775..2100
                     /locus_tag="PK710_18245"
     CDS             775..2100
                     /locus_tag="PK710_18245"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOT11725.1"
                     /translation="MSATMTLEGWGRLAMPGREVVSCDLESLTAKAVLSRGLGRSYGD
                     CSLPARPDDRVANSLMADRILGFDPGSGVLRAEAGLSLAQINRLFLPRGWFPPITPGT
                     KFVTLGGMVASDVHGKNHHRQGCFGEHVRALRIRLGDGDIVECSPNVHPDLFYGSIGG
                     MGLLGHILEVEFAMGPIPSPWIWMESSRMSSIEAFLDGLAEAALRWPMTMGWIDCLSR
                     GSSLGRGILMAGRWASVEQTGGAAFKTAAERSLPFELPNFVLNRWTARAFNALYYWSH
                     WRKRRERLVAPDPFFYPLDAILHWNRAYGPRGFTQYQCVLPRAAGPAAVREFLQRLVK
                     LGSPSPLCVIKDCGPEGSGTLSFPLEGTSIAVDMAISPDIQGIVDRLNELVIAAGGRI
                     YLTKDGFTRPDHFRAMEPRLPGFLQTKKKWDPSGRLRSAQSVRLFGDCP"
     gene            2097..2837
                     /locus_tag="PK710_18250"
     CDS             2097..2837
                     /locus_tag="PK710_18250"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT11726.1"
                     /translation="MKAVILGGTSGMGREIARRLAERGDLLFLLGPRKKNLERSAADL
                     VARHPDKVDVAFAPCDLLRPEGFSEALDAADAALGGFDTVIVTAALFATQEALEADVD
                     LTQRLVTANFANTVLFCEHARKRLLARGGGRLCVFSSVAGDRGRKPVAIYGASKAGLS
                     AYLEALDHKFHAHGLSVLCVKPGFVRTEMTAALQPPPFAGTPDRVATQVIRAMDRGKP
                     LVYSPPVWAWVMRVIRWLPRFVMRRIGF"
     gene            3274..4092
                     /locus_tag="PK710_18255"
     CDS             3274..4092
                     /locus_tag="PK710_18255"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT11727.1"
                     /translation="MKRLAWLWIVWLAGCQQVVGMDDYVFDRAEYACVDACGALEECG
                     KNGCCVAKSVRLPGGFSIDATEVTWSQYETWLSKKPSREGQPEFCGWNNTYAHAPNCM
                     NNMPKCQGASCDNLPVVCVDWCDAYAYCRAVGKRLCGKIGGGSVSESDFEDSSKGQWD
                     AACSSGGYSKYVYGDKYHAGKCHDSGEMASEVGTHPDCKSPDSHYAGVLDLNGNVSEW
                     EDSCKAYSGEDDSCRMRGGSIREAVKENNSCINTTGSQRDFSADNVGFRCCSKP"
     gene            complement(4160..>5504)
                     /locus_tag="PK710_18260"
     CDS             complement(4160..>5504)
                     /locus_tag="PK710_18260"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT11728.1"
                     /translation="RFFNAVLSHPDNGASVAPNTTYWFKVNGAVFISLNSEARATEAV
                     DAQKAWVKQVVEENPAQYYIVYTHRGFFDGSGSSVSKTSTYNLYGKWLEALQVDLVLA
                     GHEHVYVRTKPLQEGKVSSDGLGTVYITANQIGDRGRQATSTPGELCAAIHGEGSSTS
                     MISVITVSDTGIEGKMFDADGKEFDTYTIPARRAPELDSFDPQEYINAFSVEPQPPEL
                     TRGLLRFQPQGYERVRRIDLFDSQNHEWVYASFAPSQHQEQEEFGPLVPGQTYDFDLE
                     ILLKDGQTKHATLSLVNRLDAGTYDKLRVENEEGKVWLRWINHFVTSEIERIEVQVNG
                     DWSIRLAADATETDITEGIRRGSNTITFGVLDRYETWYVKETLAYEYTAPSPGTFESE
                     PTSGCAGCRTPGASPRSSAWWWILALVLLYGKRGRQRPLWHGPRKEERRKSLFYA"
BASE COUNT         1090 a         1456 c         1636 g         1322 t
ORIGIN      
        1 tgtggctcat cgtggatatg aggattttgc caactaccgt tatctgagcc aacgagacga
       61 ttcgcttctg cttgccagcc ggaagcggcg tgtgacggac accacgatcc ccgaggatca
      121 ggcatttcgt gctgtcgaag cgcgaatcgt accgatgtgg aagcatgcta gcgaggtggg
      181 ggtccggcgc ttttggcatg aatattttta cgatttggtt caagcgctcg aactaagcgt
      241 ttatgaaggg gatcccgagg catgttggct gctggaggcg gcgcgtcgac gcttgcccat
      301 tgtcgtgccc ggttatgaag attccaccta tggaaatata tttgcatcat acgtaaaatc
      361 cggtgaatgt aatgcgaata tcgtaaaatc aggtatcgaa tatatggtgg ccttctatga
      421 gcaataccaa gctctttccg caggtcctgg ggttggtttt tttcaaatcg gcggtggtat
      481 tgctggagac tttcccattt gcgtcgtacc ttccctcaag cacgatgtcg ggcagtccgc
      541 gaagccctgg gcgtatttct gccaaatcag cgattccacg acctcctacg gttcgtactc
      601 gggagccacg ccgaacgaaa aaatcacctg ggacaagctg accgaggaca cgccgatgtt
      661 cgtcatcgaa tcggatgcga ctcttgttgc gcctcttgtg ctccgagcgc tgctcgaaag
      721 caagcgggac cctgccggag cggaagcgct catcgcgcga aaggaggctg aagcatgagc
      781 gcaacgatga cccttgaagg gtgggggcgc ctagccatgc cagggcggga ggtggtttcc
      841 tgtgatctcg agtcccttac ggcgaaagcg gttttgtcac gggggctggg ccggtcctat
      901 ggtgattgtt ccttgccggc tcggcccgac gatcgggttg caaactctct tatggcggac
      961 cgaattctcg gcttcgaccc cggcagcggt gttcttcggg cggaagcggg gctgagtctg
     1021 gcgcaaatca accggctttt cctgcctcgg ggctggtttc ctcccatcac ccccggaacg
     1081 aagttcgtca ccctcggtgg catggtcgcg agcgatgtgc atgggaaaaa ccaccatcgg
     1141 caaggatgct ttggcgaaca cgttcgggcg ctacgcattc ggctgggcga tggggatatt
     1201 gtcgaatgca gtcccaacgt tcatcccgac cttttttacg gttcgattgg ggggatggga
     1261 ttgctcgggc atattttgga ggtggaattc gcgatgggac ccatcccgag tccgtggatc
     1321 tggatggaaa gcagccgaat gagcagcatt gaggcgtttc tcgacggcct tgcggaggcg
     1381 gcgttgcgat ggccgatgac gatgggctgg attgattgtc ttagccgcgg ttcgtccctt
     1441 gggcgcggga ttctgatggc ggggcgttgg gcctccgtcg agcaaaccgg tggcgccgcc
     1501 ttcaaaaccg ccgctgagcg gtctttgcct ttcgagttgc cgaacttcgt gctgaatcgc
     1561 tggaccgctc gtgctttcaa cgccttgtat tattggagtc attggcgaaa acgacgagag
     1621 cggctcgtgg ctccggaccc gtttttctac cccctcgacg ccatcttgca ttggaatcga
     1681 gcctacggtc caaggggatt tacgcagtat cagtgcgttt tgccgcgtgc ggcgggtcca
     1741 gccgcggttc gcgaatttct ccagcgtttg gtcaaactcg gctccccctc tcccctttgc
     1801 gtcatcaaag actgtgggcc tgagggctcg ggcaccctct ctttccccct ggagggcacc
     1861 tccattgcgg tggatatggc gatttcgccg gatattcagg ggatcgtgga ccggttgaac
     1921 gagctggtga tcgctgctgg cggtcggatc tacctcacga aagatggatt tacccggccc
     1981 gatcattttc gagcgatgga acctcgtctt ccaggtttcc ttcagacgaa gaaaaaatgg
     2041 gatccgtccg gacggctacg cagcgcgcaa tccgtccgcc tttttggaga ttgcccatga
     2101 aagctgtcat ccttggtggt acctccggta tggggcgtga aatcgctcgt cgccttgcag
     2161 aacggggtga tctgcttttt ttgctaggcc cccgcaaaaa aaacctcgag cgaagcgcgg
     2221 cggacctggt agctcgacat cccgataagg tcgatgtggc tttcgctcct tgcgatttgc
     2281 tgcgtcccga agggttttcg gaagctttgg atgctgccga cgccgccctt gggggcttcg
     2341 acaccgtgat tgttacggcg gcgcttttcg ctacccaaga ggctctcgaa gcggatgtgg
     2401 accttaccca acgcctggtt accgccaact ttgccaatac ggtcctattt tgtgaacacg
     2461 ctcgaaaacg gctgcttgca cgcggaggag gaaggctttg cgtgttttct tcggtagccg
     2521 gagaccgagg acgcaagcct gtcgccatct atggtgcgag caaggccggt ttatccgcat
     2581 acctcgaagc gctcgaccat aaattccacg cgcatggcct ttccgtgctt tgcgtcaaac
     2641 ccggtttcgt acgtacggag atgaccgccg cgctccagcc gcctcctttc gcgggaacgc
     2701 ccgaccgcgt cgcgacccaa gtgattcgcg ccatggaccg cggaaaaccg ctcgtgtatt
     2761 cgccccccgt ttgggcgtgg gtcatgcggg tcatccgatg gttacctcgc ttcgtcatgc
     2821 gtcgaattgg gttttaatgg cgcgcgtgac aatggaaagg gcaggaagta aaaatacctg
     2881 taatttcaat ttgttacgat gattttggaa gcgcgttgga ctgtccgccc ttcgttacgt
     2941 ttccattgcg ggaaagccac acgaaaccat gcgttgggga tgttgcaggg gtggtaaacc
     3001 gctgaccctt gtgctagtct ttgaatttct atcgacttgt tccacaagca gtaccgatga
     3061 gagtcgaccc tttttcacag gaccagaagg atgttgcgat ggatgcggaa agggctggct
     3121 tcgcgcggtg cgatagtgtc ggcgcggaat catgcctttt tcgttccaca tcgtggggcg
     3181 gatgggcacc gcagtgttcc tgacggccaa gggggcaaag gtgctgacac gctccccact
     3241 atgggtcttg ctcgggtgct caaggagaga tggatgaaac gcttggcttg gttgtggatt
     3301 gtctggctag cggggtgtca gcaagtggta gggatggatg actatgtctt tgatcgtgcg
     3361 gagtacgcct gcgtggatgc ttgtggtgcg ctggaggaat gtggaaaaaa cggatgttgt
     3421 gtggcgaaat ccgtgcgtct gcctggaggt ttttccatcg atgccaccga ggtgacgtgg
     3481 tcccaatacg aaacatggct gtctaagaaa ccttcgcggg aaggacaacc ggaattctgt
     3541 ggttggaata acacctacgc tcatgctccc aattgcatga acaatatgcc gaaatgccaa
     3601 ggggcaagct gtgacaatct ccctgtggta tgcgtggatt ggtgcgacgc ctacgcctat
     3661 tgccgggccg tcggcaagcg gttatgcggc aagatcggcg gagggtccgt tagtgaatcg
     3721 gattttgagg attcttcgaa gggccagtgg gatgcggcgt gctcttccgg aggttatagc
     3781 aagtatgtct atggggataa gtaccacgca ggcaagtgtc atgactcggg ggaaatggcc
     3841 tcggaggtcg gaactcatcc cgattgtaag tcgccagatt cccactatgc cggggtactc
     3901 gacctgaacg gcaacgtttc agagtgggaa gattcatgca aagcatattc cggagaagat
     3961 gattcctgtc gaatgcgcgg aggcagtatc agggaagccg tcaaagaaaa caatagctgt
     4021 atcaatacaa cgggctctca gcgagatttc agtgcagata acgtcggttt tcgctgttgc
     4081 tccaaaccct gagcagatag gcccaatcac cgcttttatt gtgtttttca cgatgggtga
     4141 atcctgccgg cagacgaaat catgcgtaga atagggattt gcgccgctcc tcttttcgag
     4201 ggccgtgcca caggggcctt tgacggcctc gcttcccata gagtaaaaca agggcaagta
     4261 tccaccacca tgccgaagac cggggagagg cacctggtgt ccggcatccg gcgcagccac
     4321 tagtaggctc tgattcgaac gtgccagggg atggggccgt atactcgtac gccagcgttt
     4381 ccttcacata ccacgtttcg taccggtcca acacgccgaa ggttatggta ttcgaaccgc
     4441 gacgaatccc ttcggtgata tccgtttccg tggcatcggc cgccaaacga atggaccaat
     4501 ccccgttgac ttgcacctcg atccgctcga tttcgctcgt gacgaagtgg ttgatccacc
     4561 gcaaccatac ctttccctcc tcgttttcca cccgcaattt gtcataggtc cccgcatcga
     4621 gacggttgac gagcgagagg gttgcatgtt tcgtttgccc atctttcaac aggatttcga
     4681 gatcgaaatc ataggtttgt ccgggaacga gcggtccaaa ttcctcttgc tcctggtgct
     4741 gcgatggcgc aaaggatgcg tacacccact cgtgattttg tgagtcgaat agatcgatac
     4801 ggcgcacgcg ttcatagccc tgaggctgga aacgaaggag tcctcgcgtc aactccggcg
     4861 gttggggttc gaccgagaac gcgttgatat actcttgcgg gtcgaaagag tcgagttcgg
     4921 gagcgcgtcg agccggtatc gtatacgtat cgaattcctt gccatcggcg tcgaacatct
     4981 tgccttcgat ccctgtatcg gaaaccgtga tcaccgaaat catgctggtc gagcttcctt
     5041 ctccgtggat ggccgcgcaa agctcccctg gcgtgctcgt cgcttgtctt cctcgatccc
     5101 cgatttgatt ggcggtgatg tagaccgtcc cgagcccgtc cgaggatacc ttcccctctt
     5161 gcaggggctt ggttctcacg tatacgtgct cgtgtccagc cagcaccagg tcgacctgca
     5221 aggcttccag ccattttcca tagagattat acgtacttgt tttgctgaca ctcgagccgc
     5281 tcccatcgaa gaatccccga tgggtatata cgatataata ttgggctgga ttctcctcca
     5341 cgacctgttt tacccaggct ttttgcgcgt cgacggcttc cgtggcgcgt gcttcggaat
     5401 tcaaggaaat gaaaacggcg ccattgactt tgaaccaata ggtggtgttg ggagccacgg
     5461 aggcgccatt gtcgggatgg gataatacag cgttgaaaaa tcgc
//
LOCUS       DAOSOR010000404         5468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15039_length_5468_cov_3.844449, whole genome shotgun sequence.
ACCESSION   DAOSOR010000404 DAOSOR010000000
VERSION     DAOSOR010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15039_length_5468_cov_3.844449"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            425..497
                     /locus_tag="PK710_18265"
     tRNA            425..497
                     /locus_tag="PK710_18265"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:458..460,aa:Arg,seq:cct)
     gene            868..2025
                     /locus_tag="PK710_18270"
     CDS             868..2025
                     /locus_tag="PK710_18270"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT11729.1"
                     /translation="MLHVLVGATCNNNCIFCMESDRGRRRTHILSQSADDIRRMIESY
                     PDKTEILFTSGEPTLAPRLPDYITSAHTAGYRTIALISNGRRLSYPEYTRHLIECGLN
                     KITVSIHGHRPEIHDSLTRTGGSFLQTVRGLENLAREKRRRPLDLHSSTVVVGRNLPY
                     LVDIYEFLRDKSVDRMVFNVLMLKGRGLDHIGTLMPTYRQIASKLHELAQRISAADRQ
                     RVTVADIPRCTALGLPFDIVGDQERFSQFEPSQSLGVEGLSMEDLRRMQAEGSNKMLQ
                     ASLLDVVSEGEGCLEGDSEYYLTTRELKDGLMRTKRPLCSTCIHEPGCPGVWEGYTAR
                     YGWDEFISVQADGPPGEKTNQKQRATNLPKELSSTVGTPTAGAEPRSKSEK"
     gene            complement(2075..3436)
                     /locus_tag="PK710_18275"
     CDS             complement(2075..3436)
                     /locus_tag="PK710_18275"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HOT11730.1"
                     /translation="MNPRVSRGLTRKGAWLAVFSALSVGVPFMLPWLTTACGSDDSET
                     DIVWGKTGSHDAGQDGAGGESYDAATGDSSPQGGSGGSGTGGSSGTGGSSGTGGSSGS
                     GGSGVGGSAGAAGSAGASGASGAAGSPSQPSETKKIKDLTGPNTSAAPFGVGGTDLGI
                     PVRQPNGQIAYIFGDTFEQFGVGGPGWRAPVLLRSSPGGLANGITFTSAAGGNYAKQL
                     FAYNHNDPNYTTWLPTDVITIGNRMYLHFMVNKGLGNVRWSQIAYSDDNGENWTVSSA
                     RWEGDENGNLRQLWTWERGDDGFVYVISTSFTRKDPIILHRVPEDKLLDKNAYEPWGY
                     KNNQWAWGNPATVVLTGKFGELCLRRVENKWLLSWFNAQDYDITIKVLDSPTSNLYTA
                     TTYKPIKGTAWGHENDTHVAQLYGGYIHPDSTLHELHLIISQWNTTTNWPYRAMQFVT
                     GVE"
     gene            complement(3433..3687)
                     /locus_tag="PK710_18280"
     CDS             complement(3433..3687)
                     /locus_tag="PK710_18280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11731.1"
                     /translation="MLALCFRFDWGVVVRDRVGIGTIPSSVMPDPLFGTETLEFVGYA
                     GQWPMNPQREQSWSNRLGGMTFANPCSTVFRISTLQEPLE"
     gene            3824..4906
                     /locus_tag="PK710_18285"
     CDS             3824..4906
                     /locus_tag="PK710_18285"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOT11732.1"
                     /translation="MNKQRFLGPPIAFAMLFTLGVALGNDETQARSLFREGVALMNRG
                     AFAEALDRFDRAYDLWANPKILLNIATTLRQLGRLAEAANAYQDYLNDKQADPSRTAE
                     VKKQVEEIDKNVGVLEIVVAVPGATISVYAKNPQRERGRGKSLLKVVGDQSDTESGWT
                     VRVSPGEHLVSVAKEGYQSVTESVTLAAGERRTLRLTLEIIGEKVSSPKEPPKPLPEK
                     TEAISAETEVDELGHQGQWSLYLRSDMDVSFHGGVGSAGVGYGLGKVAEVQLGALVGR
                     DKGVEPGASLYFAQGTVKPIIFIGLPVFFLEGAVPGIRGAAGLQVDASRSIGFFAHGG
                     AAAFLAVPEERKTTAIVVSAGLQGRL"
     gene            4908..>5468
                     /locus_tag="PK710_18290"
     CDS             4908..>5468
                     /locus_tag="PK710_18290"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HOT11733.1"
                     /translation="MKKGSRISAGLCILWASACQAPSESSPTAEQPLARTTSALTITP
                     VMKASHSQPNTGTTVAVSDSEAMVVDTNNNTVSVWERSGNVWLPKQDIELPNGSESIS
                     MALDGDTAVIGSIIWNPPSPKTFVYQRNGSAWTLLQTLPPTSPDQYYFGFSVAVSGSN
                     LAIGSPIDNPTSGYGAVYVYSRKNGTW"
BASE COUNT         1223 a         1558 c         1512 g         1175 t
ORIGIN      
        1 gggaagcgac cattgtccaa tatgggtgga ggtccgtcct gagatcctcg gtacttcctg
       61 aatagagtcg tatttttcgg gttcccctct tccgttcgcc acgatgaccg acggggctga
      121 gccatcccct catttttccc cggatctttc cttcacggtc ccggccctcg atcacggtca
      181 cggtcacggt cacggcccac ggcacacaaa accggtcacg gtcatagtca ccagcctttc
      241 cccttggatg cgatggtgag cgaacctcga ggatggtgaa gatcaagctg gacgacctgg
      301 gtccacgtct tgtatactat cgccccatcg gttccgccgg tagtggattt cccaggtaac
      361 ctggctctct attcggctcc cgggctcggc agaaccttcg caactcattg aaaatacaac
      421 aaaagccccc gtagctcaga ggatagagca gcggtttcct aaaccgttgg tcgcaagttc
      481 gactcttgcc gggggcgcta catcccccac aacaacgccc tgtgttccca gcccggcgtt
      541 tcttccccaa aaccagccca tgaagcctcg tattcgtctt ccccacgccg aacgtcaacg
      601 ataacggcca ggttgacgtc gtgggtggac acaacgggtc gattatatac ccaaacccgg
      661 gaagaaaggt ccttcgacag aaccaacccc tctatagttg gtgtcgaaaa tcgagcgaat
      721 catcccttcc ccacaggtat aattcgcatg aaatccgcag gaaatctacc tttgaccgcg
      781 gaggctcgat gtccaatatg ctagggtagg acccaggcaa acacgaaatt cggcctcccc
      841 aacccaagtc gatacaagag aacctcgatg cttcacgtcc tcgtcggcgc cacctgcaac
      901 aacaactgca tcttttgcat ggaatccgac cgtgggcgac gtcgcaccca tattctcagc
      961 caatctgcgg acgatattcg gcgaatgatc gagagctatc cggataaaac agaaatcctg
     1021 ttcaccagcg gggaacctac cctcgctccg cgattgcccg actacatcac atcggcccat
     1081 accgccggct accgcacgat cgccctcatc agcaatgggc gcagactgtc gtatcccgag
     1141 tatacgaggc atctcatcga atgcggcctc aacaagatca ccgtatcgat ccatgggcat
     1201 cgtccggaaa tccacgattc cctaacgcgg acgggaggct cgtttttgca aaccgttcgt
     1261 gggctcgaaa acctcgcgcg tgagaagcgt cgcagaccgc tcgacctgca ttcgagcacc
     1321 gtcgtggtcg gtcgtaatct cccctatctt gtcgatatct atgaatttct ccgggataag
     1381 tccgtggacc gcatggtttt caacgttttg atgctcaaag gccgagggct cgaccatatc
     1441 ggtacgctca tgcccaccta tcgtcaaatc gcttcgaagc tacacgaatt ggcgcagcga
     1501 atctctgccg cggatcgaca gcgggtgacc gtagcggata ttcctcgatg cacggcgctt
     1561 ggactaccct tcgatatcgt gggcgatcag gagcgattct cccaattcga accttctcag
     1621 agtttgggcg tcgagggatt atccatggag gaccttcgcc gtatgcaagc ggaaggttcg
     1681 aataagatgc tccaggcgag cttgctcgac gtggtatcgg agggggaggg ctgcctcgaa
     1741 ggggattcgg aatactatct gaccacccgt gagttgaaag atgggctgat gagaacgaaa
     1801 aggccgttat gctcgacatg catccacgaa cccggctgtc cgggcgtgtg ggagggatat
     1861 acggcccgtt acgggtggga tgaattcata tccgtgcagg ccgatggtcc gccgggcgaa
     1921 aaaacgaatc aaaaacaacg ggctacgaac ctgcccaagg agttgtcctc cacggtgggg
     1981 acaccaacgg caggagcgga accgcggtcg aagtccgaga aatgaaggcg ggaaaaaacc
     2041 aaaggctcct gcgagccgcc gcgatacggg cgggtcattc cacgccggta acgaattgca
     2101 tagcgcgata gggccaattg gtggtggtgt tccattgtga gatgatgagg tgcagttcgt
     2161 ggagcgtgga gtccggatgg atatatcctc cgtaaagctg ggccacgtgg gtatcgtttt
     2221 catgccccca ggcggttcct ttgatgggct tataggttgt ggcggtataa agattggacg
     2281 tggggctgtc gaggactttg atagtgatat cgtagtcttg cgcgttgaac caggacagca
     2341 gccatttgtt ttcgaccctc cgcaagcata attcaccgaa tttgccggtg aggaccacgg
     2401 tggccggatt gccccaggcc cattgattgt ttttgtatcc ccagggctcg taagcatttt
     2461 tgtcgagaag cttgtcctcg ggcacgcgat gcaaaataat cgggtctttc cgggtaaacg
     2521 acgtagaaat cacgtacacg aaaccatcgt cgccccgctc ccacgtccag agctgccgaa
     2581 gattgccgtt ttcgtctccc tcccaccggg cgctgctcac ggtccagttc tcgccgttgt
     2641 cgtcggaata ggcgatttgg gaccaacgca cgttgcccag ccccttgttc accatgaagt
     2701 gcagatacat gcggtttccg atggtgatca catcggtagg aagccatgtg gtatagttag
     2761 gatcattgtg gttataggcg aagagttgtt tggcataatt gccccctgcc gcgctggtga
     2821 acgtgattcc gttcgctagc cccccggggg aggagcgaag caggactgga gcgcgccagc
     2881 ctggccctcc caccccgaat tgttcgaaag tatccccgaa aatatacgcg atctggccgt
     2941 tgggttgacg aaccggaatg ccgaggtcgg tgccaccgac accgaaggga gcagcactgg
     3001 tgttggggcc cgtcaagtcc ttgatttttt tggtttctga aggttggctc gggcttccgg
     3061 ccgcccccga agccccgctg gcacccgcgc tgcccgccgc gcctgcgcta ccaccaaccc
     3121 ccgagccacc ggagcccgaa ctaccgcccg ttcccgaact accgcccgtt cccgaactgc
     3181 cgcccgttcc cgaaccgccc gagcctccct gcggtgagga atcgccggtc gccgcatcgt
     3241 aagattctcc cccagcccca tcttgcccgg cgtcgtgaga gcccgttttt ccccagacaa
     3301 tatcggtttc ggaatcatcg ctaccacaag cggtggtgag ccagggaagc ataaacggaa
     3361 caccgacgga cagggcggaa aagacggcaa gccatgcgcc ttttctggtg aggccccttg
     3421 atacacgtgg attcattcta atggctcctg aagcgtagaa attcgaaata ctgtcgagca
     3481 agggttagca aaggtcatgc ctcccaaccg gttggaccag gactgctctc gctggggatt
     3541 catgggccac tgaccagcat accccacgaa ctcgagtgtt tccgtgccaa acagaggatc
     3601 cggcatgacc gaggatggga tcgtaccgat ccctacccga tcccggacga caacgcccca
     3661 gtcgaatcga aaacagagtg caagcatgtc atatttcgca tattataaat attttctaga
     3721 tatgtatcat gatatcattc gcagaacgtg cgaatatttt gataccctcc gattcctctg
     3781 tcgattttac ggacccagtt gtgctataca tgacggcaaa ccgatgaaca aacaacggtt
     3841 tcttggacct ccgatagctt tcgcgatgct tttcacgctc ggagtcgcgt tggggaatga
     3901 cgagacgcag gcccgctcgc tcttcaggga aggggttgcc ttgatgaacc ggggcgcctt
     3961 tgccgaagcg ctcgaccgat tcgatagagc ctatgatcta tgggccaatc ccaaaattct
     4021 cctcaatatc gccaccaccc ttcgtcaatt gggacgcctt gcggaagcgg ccaatgctta
     4081 tcaagactac ctgaatgata agcaagccga cccgtcgcgc acggcggaag taaaaaaaca
     4141 agtagaggaa atcgataaaa acgtgggagt cttggagatc gtggttgccg ttcctggcgc
     4201 taccatcagc gtgtatgcca agaatccaca acgggagcgc ggacgtggca aaagcctcct
     4261 gaaagtggtg ggggaccaaa gcgacaccga atctggctgg acggtgcggg tctcgccggg
     4321 ggaacacctg gtatccgtgg ccaaggaagg atatcaatcg gtgacagaat cggtgacgct
     4381 cgcggcgggg gaacgtcgaa ctctgcgcct gactctcgaa atcattgggg aaaaagtctc
     4441 ctcgccgaag gagccaccca aaccgttgcc tgagaaaacg gaagcgattt ctgcggaaac
     4501 tgaagtcgat gagctcggtc atcaggggca atggtcgctc tatctccgtt ccgatatgga
     4561 cgtttccttc catggagggg ttggttccgc aggggtggga tatggattgg gaaaggtggc
     4621 ggaagtacaa ctgggcgcgt tggtgggacg agacaagggg gtcgaacctg gcgcttcgct
     4681 gtattttgcc cagggaaccg taaagcctat cattttcata gggttaccgg ttttttttct
     4741 ggaaggggcg gttccgggga ttcgcggggc ggcggggctc caggtcgatg cctcccgctc
     4801 aataggcttt ttcgctcatg gcggagctgc tgcgttcctc gctgtcccgg aagagcgaaa
     4861 aacaactgct atcgtcgttt ccgcaggtct acaggggagg ctatgacatg aaaaagggtt
     4921 cacggatcag cgcaggcttg tgcatccttt gggcctccgc ttgccaagcc ccttccgaat
     4981 catctcccac cgcggagcaa cccctcgcgc gtactacctc tgcgttgacg atcacaccgg
     5041 taatgaaggc atcccacagc caaccgaaca ccggcaccac ggttgccgta tcggattctg
     5101 aggccatggt cgtggatact aacaataaca cggtcagcgt atgggaaaga tcgggcaacg
     5161 tatggcttcc caagcaagac atcgaattac ctaatggcag cgaatcaatc tccatggccc
     5221 tggatgggga tacagcggtc attggttcga tcatttggaa tccaccgagc ccgaaaacat
     5281 tcgtttatca acgtaacgga tcggcgtgga cccttcttca aacccttcct ccgacgtcac
     5341 cggatcaata ctattttggg ttttccgtag ccgtctcggg ttcgaatctg gccattggat
     5401 ctcctatcga caatcccacg tcgggctatg gagctgtcta tgtttattcc cgaaagaacg
     5461 gcacatgg
//
LOCUS       DAOSOR010000405         5452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15085_length_5452_cov_4.873448, whole genome shotgun sequence.
ACCESSION   DAOSOR010000405 DAOSOR010000000
VERSION     DAOSOR010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15085_length_5452_cov_4.873448"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2646)
                     /locus_tag="PK710_18295"
     CDS             complement(<1..2646)
                     /locus_tag="PK710_18295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11734.1"
                     /translation="MTRKIFRKGLRCGVGLGLLVGLTSCAEQEAPINRVQANALDKHF
                     FLGPSLSDATDDPEFHWRGYVIDGSASQSEVGVGTWSHIDRIRWEVTENMLIARKSYP
                     LFEQPGKPGTWAGTDSDGAVVAMYRILSHFDIRRAYNSQTGEEMNVIEENTTDRPWYE
                     REYMRVDWSSNQVNDPMWMDMFFAKAFGEIQISPLVYNVTDPHHKDAPHFEPEDGYFD
                     ITNRFYIEAAETWTPWGFTIWGCYLEGLLNATATYDCDAQEATVRFSFQKIDPNNDFE
                     PLENSRASLDVVGNPGGLGSSYSVGVVTAPRIGFDPWYRFTDDNFVRFANIHNIWKKS
                     HLDFYCDAKFDNDQNGTDDQCERSVTGYSGSEGSQCDVHTKRCTIPYRDREIKTVGYW
                     VNDKMADEFLDTVDANGNPVTRGANEEIVYSWNQMMANAVAYAREVECRRTGGDRTSC
                     HNLHFAQDKEMVAFGGWLTDKALDPTPVVTLCHNPVRSYDRTEVCGPVGYEARTGDLR
                     KNMLVYWPYDSKAPYGGIANWNGDPLTGEIKGGAALIMGRSVRYAAAFARDVLQVAMG
                     DLSVSELITGVPEDVYFERSILEPGGKTMALTPEEIESRTKALDAANAMQFGGIKPLA
                     GTTLAEKMASHSRMMSQMVFDPDAEALDSAAFDALVAPLLGTKFENTIMSPSWARNTM
                     GTNGSLTADQVAMASPLRMLDPGKLRILADTVRTRLEAKGACFHDHEAPLTGSIDIPG
                     LAKHFMAKYGDLSAHERGEAIYRELAVEIYKGIALHEMGHSLGMLHQFASSWDATNYQ
                     PQYWQLRTAEGTAQASCNGQVRDPKGPDSCMGPRYLDGETADEMGLADESRPGIHYFA
                     NTSTMEYQWERFGETAGLGTYDQYTM"
     gene            complement(2690..>5452)
                     /locus_tag="PK710_18300"
     CDS             complement(2690..>5452)
                     /locus_tag="PK710_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11735.1"
                     /translation="MGDMTLDEYMAGGSHGRYAKVMSGPLPAEPLSDAEIQKRIEAID
                     TFNLAQSAPSSGGVSQSRVEVAKAAMRAKLELKADQDLRKMQALRNDQLLKPLRNTPL
                     EHDLMDAAWLKAAKLDPSAQLTDSVMNAASPLRGLDAAKMQLIRREMEERFARAGACF
                     SEMQQTAQMGQIVNAPLANYFLQKYGHLSREERIAEMRKELRLESFKGVMIHEMGHAL
                     GMRHQFSSSWDSTNYMPQYWQLRTNEGQATASCEGKARDPNAEDSCMGPRYLDPHTLD
                     EQGYGPEPRPMIEYFANTSTMEYQSERFSETAGLGTWDYHMTKAIYGRVLETYDNDVI
                     PRRDPDKPVDQYRLASRMFTQLSESELVYDSVTFDNGSQPFGPDPFMHSWHYTKLNRA
                     LNTFNPERDCREATPEEKAQAKWRVVHDKVCQPFPRDYASWYDFVSDSCPTVYDEEPM
                     VLWHTRSDLPGGNADTVRWSYRIGEHYSPTYVHTNMLDSGADTYEVAVNEIRNFNANY
                     PSVYFRRHRRGSPPSWGAAGYAMDTFELLRSYHWSIANTNLRWLSFGKQYYEIFSSDD
                     NWSRPDVMANTEIFNALASYVLAPQPGDYRPRAGDPSGVYDALPWVDPGADFTIQTID
                     GRYVDDAYDSGPNGGGSWEFEDWETRAGFWAEKAYAFLALCDSRPTLSTIAREVYLDD
                     RGVKLNFRSDMPEAFDRLFGGMLAEDWQAIGMWVDNNNQPGQPAIPAMLDLMNKGEPT
                     RPAGAKVLYPNLGYMQQLYASIFSALYARENTDMTLIHKMRIWIDGVEGNISDAAFPD
                     EKDEGGSEGRTYQVRFYHPGSGFTYIARSFGAENIDGRQVDRGIASRMLQRANQLVTL
                     SYVVKKEQGKPVFDKYGRPELILDAQGQPQPLDPNESKIGELTRYVGLIDAVRQLGHI
                     LGQGPY"
BASE COUNT         1182 a         1612 c         1455 g         1203 t
ORIGIN      
        1 catcgtatat tgatcatacg tgccaagccc agccgtctct ccaaaccgct cccattgata
       61 ctccatcgtg gaggtgttgg cgaagtagtg aatacccggc cgtgattcgt ccgctaaccc
      121 catctcgtcg gcggtttccc catccaggta acgggggccc atgcaagaat ccggaccctt
      181 cggatcccga acttgcccgt tgcaagaggc ctgcgccgtc ccctcggccg tccgcaattg
      241 ccaatattga ggctggtagt tggtcgcatc ccaagaggag gcgaattggt gcaacatacc
      301 aagcgaatgg cccatttcgt gaagggcaat tcccttgtaa atctctacgg ccaactctcg
      361 atagatcgct tcaccacgct catgcgcaga aaggtcgccg tacttcgcca tgaagtgctt
      421 ggctagaccc gggatatcaa tgcttcccgt caaaggagcc tcgtggtcgt ggaagcacgc
      481 acctttggcc tccagacgcg tccgtaccgt atccgccaaa atccgcaact tgccggggtc
      541 cagcatccgc aaaggcgacg ccatggcgac ctgatccgcc gtcagagacc cattggttcc
      601 catcgtattg cgggcccaag atggactcat gatcgtgttt tcgaacttcg ttccgagcag
      661 cggcgccacg agcgcgtcga aagccgccga atccaacgcc tcggcgtcgg gatcgaaaac
      721 catctgggac atcattcggg aatgagatgc cattttctcg gccagcgtcg ttcccgccaa
      781 cggcttgata ccgccaaact gcatggcatt ggctgcatct agcgctttgg ttcgactttc
      841 aatctcctca ggcgtcagcg ccatcgtctt gccaccaggc tcgagaatgc tccgttcgaa
      901 gtaaacatcc tccggaacgc cggtgatcag ttcggagaca ctcaagtccc ccatcgctac
      961 ttgcaacaca tcacgagcga aagccgccgc atagcgaacg gaacggccca tgatgagcgc
     1021 agcgccaccc ttgatttcgc ccgtgagtgg atcgccgttc caattggcaa tgcctccgta
     1081 cggtgccttg gaatcataag gccagtacac gagcatgttt ttgcgtaaat cacccgtacg
     1141 agcctcatac cccacaggac cacaaacctc ggtccggtca tagctccgca ccggattgtg
     1201 acaaagcgtt accaccggag tcgggtcaag agccttgtcc gtcaaccagc ctccaaacgc
     1261 caccatctct ttatcttgag caaagtgcag attgtggcag gaggtgcgat cacctccggt
     1321 acgacggcac tcgacttcac gtgcatacgc gacggcgttg gccatcatct ggttccacga
     1381 gtacacgatt tcctcgttgg caccgcgggt cacggggttt ccattggcat ccactgtgtc
     1441 gaggaactcg tcggccatct tatcgttgac ccagtagcca accgtcttga tttcacggtc
     1501 acgatacgga atcgtacacc gcttcgtatg aacgtcacat tgcgaccctt ccgagccaga
     1561 atatcccgtc acggatctct cgcactggtc gtccgtgccg ttctggtcgt tatcgaactt
     1621 ggcgtcgcag taaaagtcca gatggctctt tttccaaatg ttatggatgt tggcgaaacg
     1681 aacaaaattg tcgtcggtaa agcgatacca agggtcgaac ccaatccggg gggccgtcac
     1741 cacgcccacg gaatagctgc ttccgagacc accggggttg cctacgacat ccaacgacgc
     1801 ccgtgagttt tccaacggct cgaaatcgtt attcgggtcg attttctgga acgaaaaccg
     1861 gacggtagct tcctgcgcat cgcaatcata cgttgccgtc gcgttcaaca atccttcgag
     1921 gtagcagccc cagatcgtga atccccaggg cgtccacgtt tccgccgctt cgatataaaa
     1981 ccgattggta atatcgaagt aaccatcttc cggctcgaag tgcggggcat ctttatggtg
     2041 cggatccgtc acgttataca caagcggact aatctggatt tcaccaaagg ccttggcgaa
     2101 gaacatatcc atccacatcg ggtcattgac ctgattagag gaccaatcca cacgcatgta
     2161 ttctcgctcg taccatggcc gatcggtagt attctcttcg ataacgttca tctcctcgcc
     2221 cgtctgagaa ttgtaggcgc gacgaatgtc aaagtggctg agaatacgat acatggcgac
     2281 gacggcgccg tcggaatccg tgccggccca ggtaccgggt ttaccgggtt gctcgaaaag
     2341 cgggtaagac ttgcgggcaa tgagcatatt ttcggtgact tcccagcgaa tcctgtcgat
     2401 gtgggaccat gtgccgacac ctacttcgct ttgggaagcg gaaccatcaa tgacatagcc
     2461 acgccagtga aactcggggt cgtcggtcgc atcagacaag ctgggcccga ggaagaagtg
     2521 tttgtcgagt gcattggctt gcacgcggtt gatgggcgct tcttgctcag cacagcttgt
     2581 caagccgacg agcaacccga gccctacgcc gcagcgaagt ccttttctga aaatcttgcg
     2641 ggtcatcgtt catactccgt tgaacatggt gccaactgcc tcatgttcct cagtagggcc
     2701 cttgaccaag gatatgaccg agctggcgaa cagcgtcgat caatccgacg tagcgagtca
     2761 actcgccgat ttttgactcg ttgggatcga ggggctgcgg ttgcccctgg gcgtcgagga
     2821 tgagttcggg gcgtccgtac ttgtcgaaaa cgggcttgcc ttgctccttc ttgacgacgt
     2881 acgaaagagt cacgagctgg ttcgcccgct gtagcatccg ggaagcgatc ccgcggtcca
     2941 cctgccgacc gtcgatgttt tccgccccga agcttcgggc gatataggtg aacccggatc
     3001 cgggatggta gaaccggact tgatacgttc gcccttccga accgccttca tccttctcat
     3061 ccgggaaagc agcgtcagag atattgccct ccacaccgtc gatccaaatt cgcatcttgt
     3121 ggatgagggt catatccgta ttttctcggg cgtagagagc cgagaaaatc gatgcataaa
     3181 gctgctgcat atagccaagg ttcggataaa gtaccttcgc gcccgcgggt cgagtcggct
     3241 cacccttgtt catgagatcc aacatcgcag gaatcgccgg ttgtccgggt tggttgttgt
     3301 tgtcaaccca cattccgatg gcttgccaat cctccgcgag catcccaccg aaaagccggt
     3361 cgaaagcttc cggcatatca ctgcggaagt tcagtttcac accacgatcg tcgagataca
     3421 cttcccgcgc aatcgtcgat agagtcggtc gagagtcgca aagcgcaagg aaggcatagg
     3481 ctttctcagc ccagaaacca gcgcgggttt cccaatcctc gaactcccag gaaccaccac
     3541 cgttcggccc agagtcatag gcgtcatcca catagcgccc atcgatggtc tgaatcgtga
     3601 agtccgctcc cggatccacc cagggcaagg catcataaac accggagggg tctcccgcac
     3661 gcggacggta gtcgcccggc tgaggcgcga ggacgtacga tgccaaagcg ttgaaaatct
     3721 cggtgtttgc catgacatcg ggacgagacc agttgtcgtc gctggagaat atttcgtagt
     3781 actgtttacc gaagctcaac caacgtaggt tggtattggc aatggaccag tgatacgacc
     3841 gtagcaattc gaatgtatcc atggcatacc ccgcggctcc ccaactgggg ggcgagcccc
     3901 tacgatgcct acggaagtaa accgagggat agttggcatt gaagttgcgg atttcgttga
     3961 ccgccacttc gtaggtatcc gctcccgaat ccaacatatt cgtgtggacg taggtgggag
     4021 aataatgctc gccgatgcgg tacgaccaac gaaccgtatc cgcgttccca ccgggcaagt
     4081 ccgagcgcgt atgccacaac accatcggct cttcatcgta taccgtggga caggaatcgc
     4141 tgacgaaatc ataccacgag gcgtaatcac gcgggaaagg ctgacaaact ttgtcatgga
     4201 ccacacgcca cttggcctga gctttttctt ccggcgtggc ttctcgacag tcgcgctccg
     4261 gattgaaggt gttcaaggct cgattcaact tcgtgtaatg ccaactatgc atgaaaggat
     4321 ccggcccaaa cggctgggag ccattgtcaa acgtcacaga gtcatacacg agttcgcttt
     4381 ccgaaagctg cgtgaacata cgggaagcga gacggtactg gtcgaccggc ttgtcgggat
     4441 cccggcgggg aatcacatcg ttatcgtagg tttccaagac tcggccgtaa atcgccttcg
     4501 tcatatggta atcccacgtt cccaatcccg ccgtttccga gaaacgctca gattgatact
     4561 ccatcgtcga ggtattggcg aagtactcaa tcatcggtct gggttcggga ccatagccct
     4621 gctcgtcgag cgtgtgcggg tcgagatagc gcggacccat gcaactatcc tcagcgttcg
     4681 ggtcccgagc ttttccctcg cagcttgccg ttgcctggcc ttcgttggtt cgaagttgcc
     4741 aatactgggg catgtagttg gtggagtccc acgatgagga gaattggtgg cgcatgccga
     4801 gggcgtgacc catttcgtga atcatgacgc ccttgaagct ctctagccga agctccttgc
     4861 gcatttcagc gatacgctct tcgcgggaaa ggtgcccata cttctgtaag aagtagttcg
     4921 ccagcggagc gttcacaatc tgccccatct gcgccgtctg ctgcatttcg gagaaacagg
     4981 cacccgcgcg cgcaaaccgc tcttccattt cccttcggat gagctgcatc ttcgccgcat
     5041 ccaagccacg caagggggaa gcggcattca tgaccgaatc ggtgagctgc gccgaaggat
     5101 ccaacttggc cgccttgagc cacgccgcgt ccatcagatc gtgctccaat ggggtgttgc
     5161 gcaacggctt caagagctgg tcattgcgca acgcctgcat tttgcgcagg tcttgatccg
     5221 ctttgagttc aagtttcgcg cgcatggccg ctttcgcgac ttcgactcga ctctgcgaga
     5281 ctcccccgga ggaaggagcg gattgagcca aattgaaagt gtcgatcgct tcgatcctct
     5341 tttggatctc cgcgtccgat agcggctcgg ccggtagcgg cccactcatg actttcgcat
     5401 aacggccatg agacccacca gccatatact catccaacgt catgtctccc at
//
LOCUS       DAOSOR010000406         5437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15143_length_5437_cov_3.821256, whole genome shotgun sequence.
ACCESSION   DAOSOR010000406 DAOSOR010000000
VERSION     DAOSOR010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15143_length_5437_cov_3.821256"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1364)
                     /locus_tag="PK710_18305"
     CDS             complement(57..1364)
                     /locus_tag="PK710_18305"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF007128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HOT11736.1"
                     /translation="MATLQEKLQELIPQWRTERAKILKEYGDKELSKATVTMAFGGMR
                     GIKGMICDTSVVEPDKGLIIRGYPLLDIMDLWPEQTFCLLLTGKIYDLDDPIIKNYQK
                     EMDRRSQIPQYVYDVLEAMPRDSHPMCMLNTGILVMEKESSFRAAYTKGTKKEDYWVS
                     TLDDSMTLLARLPGLAAAVYRLRYEKGDYIHWTPGLDWGANYANMLGISDPTGEFAKL
                     MRLYLHFHCDHEGGNVSANTCHTVGSALSDAYYAVSAGLNGLAGPLHGLANQECLGWV
                     LELMEKFGGTPTEEQIRNTAFETLRAGQVVPGYGHAVLRVTDPRFTGFNKFGKQYLAN
                     DPVFMTVDRVFNVVPKVLEEYSAERVKAGKGPIANFWPNVDAGSGALLYHYGLTEFEY
                     YTVLFSVSRAMGMLSQLVWNRAVGTAITRPKSVSMEWITKNVK"
     gene            1666..2616
                     /locus_tag="PK710_18310"
     CDS             1666..2616
                     /locus_tag="PK710_18310"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOT11737.1"
                     /translation="MTTHDTLAPIAAPPPPPRYRRLKWLWIPAILAAPIAVWGIVYAQ
                     RPEPQRSSPHALQALVPRHGEPLPTPAVSSATAAHDPSRSGMVAEEAREAQDDLAQEQ
                     GSTGGEQALNEGEESEEIASEEDEAREQKPKSATVSEGKFWIQLASLPLRENAERLAA
                     KLRKRGHDAQAMAYGGPSAGWWHVVRLGPYESRMDAEKARLDFVKAEPMKTEVIPRAR
                     GEYQIQLASVRSEKQAQTLVRRLSRQGHPAALRTINTAKGVWYAVRVGPFDSKQDAIG
                     YRHLLEEYSHVTGDLLPREKFAAPLPDAEDESDSADRMRE"
     gene            2763..3305
                     /locus_tag="PK710_18315"
                     /pseudo
     CDS             2763..3305
                     /locus_tag="PK710_18315"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            3367..4149
                     /locus_tag="PK710_18320"
     CDS             3367..4149
                     /locus_tag="PK710_18320"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT11738.1"
                     /translation="MRAAAENLTPVTLELGGKSPCIIDASADLDVTARRITWGKFFNA
                     GQTCVAPDYLLVHSSVHDALVARIKATIQQFYGADPKESPDYARIVNLRHHRRLCALL
                     GSGDVAVGGEADEENLYLAPTLLTHVPADSAVMAEEIFGPILPVLSISSIDEAIHFVK
                     AREKPLALYVFSKDEAVQRRVIHSTSSGGVAVNHVWLHLAVPGLPFGGVGQSGMGAYH
                     GRWTFETFSHRKAVLVKSTKVDPTLFYPPYTELKARWIRRLL"
     gene            4166..4957
                     /locus_tag="PK710_18325"
     CDS             4166..4957
                     /locus_tag="PK710_18325"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOT11739.1"
                     /translation="MGMHPFLQTLRPTLHIAHRGGAALAPENTVEAFRLALDRYHTDM
                     IETDVHMTADGVLVLMHDATIDRTTEGTGVVADFTIDRLESLDAGYRFRSLTGEFTFR
                     GRDVRIPRLETVLQLFPTTRFNIEIKDDRPGVEDAFAQIIRNAGAIDRVCVGSEDDEV
                     SRRLLGALPEACHFYPRHAAAIFIAALRRGEPCENDPYEVLDIPLWFGETRLVDASLL
                     AGALERNRWVNVWTVDERSEMEKLVADRVGGIMTDRPDRLRDVLG"
     gene            5138..>5437
                     /locus_tag="PK710_18330"
     CDS             5138..>5437
                     /locus_tag="PK710_18330"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOT11740.1"
                     /translation="MADERNERSDLCSGNVTLASVPNDTPPRTEKKKSLRHELRTPLN
                     QIIGYSELLQEDAEEHGLGAFVEDLKKIERAGRHLLELINSIADSDTTPTPVVPSH"
BASE COUNT         1157 a         1542 c         1527 g         1211 t
ORIGIN      
        1 aaatgaggaa cggctccaag aaggagccgt tgctacatca cgttacgaag attggattac
       61 ttcacgttct tcgtgatcca ctccatgctg acggacttgg gacgggtgat ggcggtgccg
      121 acggcgcggt tccagacgag ctgggacagc atgcccatgg cgcgggagac ggagaacagg
      181 acggtatagt attcgaattc ggtgaggccg taatggtaga gcagggcgcc ggagccggcg
      241 tcgacgttcg gccagaagtt ggcgatgggg ccttttccgg ccttgacgcg ttcggcggaa
      301 tactcttcca agaccttggg aacgacgttg aatacgcggt ccacggtcat gaagacgggg
      361 tcattggcga ggtattgttt gccgaatttg ttgaagccgg tgaagcgggg gtcggtgacg
      421 cgaagcacgg cgtggccgta tccggggacg acctgtccgg cgcgcagggt ttcgaaggcg
      481 gtgttgcgga tttgctcttc ggtgggggtg ccgccgaact tttccatgag ttcgaggacc
      541 cagcccaggc attcctggtt ggcaagaccg tgaagggggc ccgcaaggcc attcaacccc
      601 gcagatacgg cgtaataagc gtcggagagg gccgagccga cggtatggca ggtgttggcg
      661 gaaacgttgc cgccttcgtg gtcgcagtgg aagtgcaggt acaggcgcat cagcttggca
      721 aactcgcccg tggggtcaga aataccaagc atattcgcat agttagcacc ccagtccaga
      781 cctggagtcc agtggatata atcacctttt tcatagcgga gacgatagac cgccgccgcg
      841 aggcctggca gtctcgcaag caaggtcatc gagtcgtcga gcgttgaaac ccagtagtct
      901 tccttcttcg tgcctttggt gtaggcggcg cggaaggaac tctccttttc cattaccaaa
      961 atgcccgtgt tcaacatgca catgggatgg ctgtcccggg gcatggcctc gaggacatcg
     1021 tagacgtatt gcgggatctg ggaccttcgg tccatctctt tttggtagtt tttgatgatc
     1081 ggatcgtcga gatcgtagat ttttccggtc aagagcaggc agaaggtttg ctcgggccat
     1141 aggtccatga tgtcgagcag tggatagcca cggataatca ggcctttatc cggttccacc
     1201 accgaggtgt cgcagatcat gcctttgatg ccgcgcatgc caccgaacgc catcgtcacg
     1261 gtcgccttcg agagctcctt atcaccgtat tctttgagga ttttggcacg ctcggtgcgc
     1321 cattggggaa tgagctcctg aagtttctct tgcaacgttg ccatgttggt ctcctttact
     1381 cgcccatgca cgttcgtcat ttcatcgcga acggtagcca gccggacacg attgaacccg
     1441 ggagtgagcc cccactgagc ccatctcgac gcccatccta tcccctcatt tgcagtggct
     1501 gtcaacacga tccgcgcatc atcgcgtggg tcccccagcc aacatagacc aaacccattc
     1561 gcaccatacg ccgatgagtt gagcgattca agagcctgat gcggagtcct ttgcccaacg
     1621 cagacaattt gcctatttct ttaggagaat gtcacacttg gccccgtgac gacccacgac
     1681 accctcgccc ccattgccgc tcccccccca ccgcctcggt atcgtcgcct gaagtggtta
     1741 tggattccgg cgattcttgc ggctcccatc gccgtttggg ggatcgtcta tgcccaacgg
     1801 ccagagcccc aacgctcatc tccccacgcc cttcaagctc tcgttcctcg acatggggaa
     1861 ccattaccca cgccagccgt ttcttcggca actgccgccc acgacccgtc gcgcagcgga
     1921 atggtcgcgg aggaagcacg agaggcgcaa gacgatcttg cgcaagaaca aggctcaacg
     1981 ggtggagaac aagccctgaa cgagggggag gaaagcgaag aaatagcttc ggaagaagat
     2041 gaagcccgag agcagaagcc gaaatcggct accgtgagcg aagggaagtt ttggatccaa
     2101 ttggcctcct tgcctcttcg tgaaaacgcc gaacgtcttg cagccaagct tcgcaaacga
     2161 gggcatgacg ctcaggcgat ggcttacggt ggacccagcg cgggatggtg gcacgtcgtt
     2221 cgtcttggtc cctacgaatc aagaatggac gccgaaaaag ctcgtctcga tttcgtcaaa
     2281 gccgaaccga tgaaaaccga agtcattcct cgcgcgcggg gcgaatatca gatccaactc
     2341 gcttctgttc gctcggaaaa acaagctcaa acgctcgttc gacggctttc tcgacaaggt
     2401 catcccgctg ccttgcgaac catcaacacc gccaagggcg tgtggtacgc cgttcgagtc
     2461 ggtcccttcg actccaaaca agacgccatc ggttatcgac accttctcga agaatattct
     2521 catgtgacgg gtgatctatt gccccgagaa aaattcgctg cccccttgcc tgacgccgaa
     2581 gatgaatccg attccgcgga tcgaatgcgc gaataggcca tctacctcat gcatgcctgc
     2641 cgatacgcgt tgcgatcgaa atactggtat cgtctctttt tcacgtttac tcacttgccc
     2701 tccccacgcc aagagctatc ttcctcgacg gtttttcttg ttgtgaatcg tttggtgaaa
     2761 acatgcacga ttctacatcc tctcccgatt acaccaccct catcggcacg cttcgcactt
     2821 cgtttggtgc gggacggtca cgaccgatct cgtggcgcat ccaacaactc agcgcgatcg
     2881 atcatctcgt gggtcaacat gaacgggaga ttaccgatgc tctgcatgcg gacgtgggaa
     2941 agccttttct cgaaagcctc ggcgccgaat tgaccttttt gcgatccgaa gccaagtatg
     3001 ccattcgtcg cctgcgtctc tggacccgaa aacgtaaggt caatagccca gtcgctcttc
     3061 agcctgccaa aagctatatc cagcctgaac cgttggggtt ggttctcatc atcgggccgt
     3121 ggaactatcc ttttcaactc atcctgagtc ctctgctcgg tgcgatcgcc gcgggcaatt
     3181 gcgcgatcgt caaaccctcc gaacttgctc ccgctacttc ctctcttctc gcacaactca
     3241 tcggacaata tctggatacc tcctgtatcc gcgttgtcga agggggaatt gcggagacca
     3301 cccaactgct aacaacgctt cgaccatatt ttctatacgg gaaacggcac ggtaggacga
     3361 atcatcctgc gcgccgccgc cgagaacctc acccccgtca ccctcgagct tggcggaaaa
     3421 tctccgtgca tcatcgatgc ttccgcggat ctcgacgtca ccgctcgccg cattacctgg
     3481 ggcaagttct tcaatgcggg acaaacctgt gtcgcacctg attacttgct cgtccactct
     3541 tccgttcacg atgcgctcgt cgcgcgaatc aaagcaacga ttcaacagtt ttatggcgcg
     3601 gatccgaagg agagtccgga ttacgccagg atcgtgaacc tacgacacca tcggcgcctt
     3661 tgcgcgttgc ttggcagcgg cgatgtcgcc gtgggaggcg aggccgacga ggaaaaccta
     3721 tatctcgctc ccaccctctt gacccatgtc cccgcggatt ctgcggtcat ggccgaggag
     3781 atctttggtc caatccttcc cgtgctgtcg atttcttcga tcgacgaggc gattcacttt
     3841 gtgaaggcac gggaaaaacc gcttgcatta tacgtattta gcaaagacga ggcggtgcaa
     3901 cggcgggtga tccacagcac gagttccggc ggcgtagccg tgaatcatgt gtggctgcat
     3961 ctcgccgttc ctggattacc ctttggcggg gtagggcaga gcggcatggg ggcctatcat
     4021 ggacgatgga cgtttgaaac gttcagccac cgaaaggcgg tgttggtgaa gtccaccaaa
     4081 gtggatccga ctctgtttta ccctccctat acagaattga aagcgcgctg gattcgtcgc
     4141 ttgctctgat cagcacgaga tatccatggg aatgcatcca tttttgcaga ccttacggcc
     4201 aacgctgcat atcgcccatc gtggaggcgc ggccctggct cccgaaaaca cggtcgaagc
     4261 ctttcgactc gctctcgatc ggtatcacac cgacatgatc gaaacggatg tgcatatgac
     4321 cgccgatggc gtgttggtgc tcatgcacga cgccaccatc gatcgaacga ccgagggaac
     4381 gggggtggtg gcggacttca cgatcgatcg actcgaatcg ttggatgcgg gttatcgatt
     4441 ccgatcgttg accggtgagt tcacctttcg gggtcgagat gtccgtatcc cacgattgga
     4501 aaccgtgctc caattgtttc caactacgcg atttaacatc gaaattaaag atgatcggcc
     4561 tggggtcgaa gatgctttcg cgcagatcat ccgaaacgct ggcgccatcg atcgcgtttg
     4621 tgtgggtagc gaagatgacg aggtgtctcg tcgcttgctt ggcgcgttgc ccgaagcctg
     4681 tcacttttac ccccgtcatg cggcagcgat attcatcgca gctctgcgaa ggggggaacc
     4741 ttgcgaaaac gatccctatg aggttctgga cattcccttg tggttcggcg aaacgcggct
     4801 cgtcgacgca tctctcctgg caggggctct cgagcggaac cgatgggtaa acgtgtggac
     4861 ggtggatgag agaagcgaga tggagaagtt ggttgcggat cgagtcggag gcatcatgac
     4921 ggatcggccc gatcggcttc gtgatgtgtt gggttgaggg gcacgcattg cctttgttct
     4981 cgttgagatc ggaaatactc ggtagttaga ggcgcatctc ctggggtcgt agcctttctt
     5041 ccaacaatgg ctggccgaaa agcggttcac ggtactcaag cgagctttag cgcggtacga
     5101 taggatttcc tgattgtgtg ggaatccgag tcatcctatg gccgacgaac gaaacgaacg
     5161 aagtgatctc tgttccggca acgtaacgct cgcttccgtt cctaacgaca cgcctcccag
     5221 aacggagaaa aagaaaagtc ttcgtcatga gcttcgcaca ccgctgaatc agatcatcgg
     5281 atatagcgaa ttgctgcagg aggatgcgga agaacatggc ctgggggcgt tcgtggagga
     5341 cctgaaaaaa attgaacgcg cagggcgcca tctgttggaa cttatcaatt ctattgccga
     5401 ttccgataca acacccactc cggtcgtccc ctcccac
//
LOCUS       DAOSOR010000407         5431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15162_length_5431_cov_4.884301, whole genome shotgun sequence.
ACCESSION   DAOSOR010000407 DAOSOR010000000
VERSION     DAOSOR010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15162_length_5431_cov_4.884301"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..1384
                     /locus_tag="PK710_18335"
     CDS             119..1384
                     /locus_tag="PK710_18335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11741.1"
                     /translation="MPNQATVDWTAGKKTVADLGEIRKEYSEVREFVASPDGERIAVV
                     VRKDDDVVGVCVNGQVWEQDLEKAWHLAFGPDNRLTALVRVDDEWTVAVDGELWEERY
                     EFVWNTKFSADGSRIAAQMKQDMRYGVSVNGQAWETLYASCRDYALSGDGESVAALVQ
                     VEDLPEADIFKFLEGTWSVAVNEQPWERKYLNVYAPCFDETGKRVAVEVRTDATQYTV
                     AEDDEPWETKYTCVWEPSFRPGHRGIVVPVRVSGAWTLAEGGKPLWKNRFMQLWRQRF
                     SPDGKRIAAVVAPSYGRWTIAVDDVAWDQTFGDMVLAPLFSPDGKHVAATVKQENRWG
                     VAVDGRAWSETFDMVWEPVFSPEGDVVLAKVERDGRYRLVANDRLWKPTFELLWDPVV
                     SPDGKHVLVRGVVDEKYFRQVVSLGEIMG"
     gene            1400..2044
                     /locus_tag="PK710_18340"
     CDS             1400..2044
                     /locus_tag="PK710_18340"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HOT11742.1"
                     /translation="MESYWIPFVWLAFVGLLGGTAFKIVQMGRLASREKTVFPTLDAK
                     HGARSVLHWLLPFGTRNMRLRPFFTVVSFAFHACLLITPLFVMGHAVLWQQSWGISWW
                     SLPAPVADFMSLVVVAGGLFFILRRIAAPQVRNVTTWSDYAIVLLVIAPFVTGFVAHQ
                     GWLPSKHAIALHIASGIAWLLAIPFTRLAHMFWFVFSRAYMGSEFGAVRNARDW"
     gene            2060..3376
                     /locus_tag="PK710_18345"
     CDS             2060..3376
                     /locus_tag="PK710_18345"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOT11743.1"
                     /translation="MSDPTDMMIADAGIDEGIKKLTPERIEQTIRHVLDNESAARMKV
                     YLETCVHCGLCAEACHTYNSRDRDPDYAPVAKVKNTLWEMVKRKGKVDGPFLKNAARI
                     AFTECGACRRCSMYCPFGIDIAYQIMTVRRICSLLGLVPQYLQDTTNSHAVTSNQMWV
                     QPDDWIDTLMWQEEEAMSELSGARIPLDKEGADVFYSVIAPEPKILAQLIGNMAVIMK
                     AANIDWTMPSADGWDNSNMAMYSGDFEVMGRVERAHWERAAQLRVKRIVMGECGHAYR
                     GAVYDGPKWLAWKQPPIPMVHAVEFYYELIKSGKLVIKRKIEEPVTVQDPCNIVRGRG
                     LHDKLRYVIKALCEDFRDMEPRHEHNYCCAAGGGVINCGPPWKLSRMKSIRCKADQLA
                     ATKASLIITPCHNCHSGIEDIIGHYKLGMHVKFINELLVKAIDLES"
     gene            3391..4893
                     /locus_tag="PK710_18350"
     CDS             3391..4893
                     /locus_tag="PK710_18350"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOT11744.1"
                     /translation="MRISRALTANWMVVVVIACLAVLGCSDKDKPILGPSTIVLRLSP
                     QWGALDRPAVDFDHAKHTDALGPKSCVSCHTQDSAGVMKYAWAATAASAKEAMNTFHD
                     GCLNCHQDRASKKQKTGPVACGSCHAHAPGTSQEQAHLRFDQSLHHRHEKAEDGKCET
                     CHHVWDKAQGKFLPKKGEESACADCHGAKDEGRVLSLQHASHDRCVQCHLQREEKQQP
                     TGPTLCVGCHDAESIAAIKRVDPVPRLMRGQPNHTWIHADGATAAYVPFDHAQHEAAT
                     SRCSSCHHKTMRACKDCHTLTPTSEGGYVSLERAYHEPSSTFSCVGCHKKETQRPGCV
                     GCHQTIPGEPREASCTTCHAGPTTKAMKADLPEPTLATANLEDLPPTSAAFPATLRLE
                     YFPGGDYGPADLPHKRIVEHLDREVRKDKMATRFHGTMETLCAGCHHHSPVGVQPPKC
                     VSCHPADADLSRDRPGLKVALHRQCILCHQPMGIKAGCVDCHAENKEQKP"
     gene            4890..>5431
                     /locus_tag="PK710_18355"
     CDS             4890..>5431
                     /locus_tag="PK710_18355"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT11745.1"
                     /translation="MSVSRRGFLTGLATGAGMLTAHKANAAGVQQFTGYQGRFGLLHD
                     TTLCVGCRSCEKACKEVNGHPPPDRPIDDKTVFDRHRRITPTEFTVVNRYKPAEGRTP
                     PVYRKHQCMHCNEPCCASVCLVHAFEKTPEGPVLYDPDVCLGCRYCVMACPYHALSYE
                     YDSAFDPKVMRCTMCYPRIK"
BASE COUNT         1157 a         1460 c         1665 g         1149 t
ORIGIN      
        1 cttcgcttga ctcaattctc aaattgccct acttgcctaa tggcagcctg tcgcgaaacg
       61 cgacgcgggc agggattgac tgcgttcttg aagctgaagc gcaaggagca agagggcaat
      121 gcccaatcaa gctaccgtgg attggacggc tggcaaaaag accgtcgccg acctgggaga
      181 gatacgcaaa gagtactctg aggtacggga attcgtggcg agtccggacg gtgagcgaat
      241 tgcggtggtc gtccgcaaag acgatgatgt ggtgggggtt tgcgtcaacg ggcaggtctg
      301 ggaacaggat ttggaaaaag cctggcattt ggcgtttggc cctgacaatc ggctgacggc
      361 gttggtgcgg gtcgacgatg agtggacggt cgcggtggat ggggagttat gggaagagcg
      421 gtatgagttc gtgtggaaca cgaagttcag tgcggacggt tcccgtatcg cggcccagat
      481 gaaacaggac atgcgttacg gtgtatccgt aaacggacag gcttgggaaa cgctctatgc
      541 ttcttgtcgg gattacgcgt tgagtgggga tggagaatcg gtggcagcgc tggtgcaggt
      601 ggaggatttg ccggaagcgg atatcttcaa attcctcgaa ggcacatgga gcgtggcggt
      661 caacgagcag ccgtgggagc gaaaatactt gaacgtatac gcaccctgtt tcgatgaaac
      721 cggcaagcgt gtcgcagtcg aggtgcggac ggacgccacg cagtacacgg tcgcggagga
      781 tgacgagccg tgggaaacga aatacacgtg cgtctgggag ccatcgtttc gtccgggtca
      841 tcgaggcatt gtggtgccgg ttcgtgtcag cggggcgtgg acgctcgcgg aggggggaaa
      901 accgttgtgg aaaaaccgct tcatgcagct ttggcggcag cgtttcagtc cggatggcaa
      961 gcgaatcgcc gccgtggtcg cgccgtcgta tgggcgatgg acgatcgcgg tcgatgatgt
     1021 agcgtgggat cagacgttcg gggatatggt tcttgccccc cttttctcac cggatggcaa
     1081 gcatgtggcg gcgacggtga agcaggagaa tcgttggggg gtcgcggtgg atggtcgagc
     1141 ctggtccgag acattcgaca tggtgtggga gccggttttt tcgccggagg gggatgtggt
     1201 gttggcgaag gtcgagcggg acgggcgcta tcgattggtg gccaacgatc gtctttggaa
     1261 gcctacgttc gagcttcttt gggaccccgt ggtgagtccc gatggaaagc atgtgttggt
     1321 tcgtggtgtt gtggatgaga agtacttccg ccaggtggtt tccttggggg agatcatggg
     1381 ctgacttgga ggcagggccg tggaatcgta ttggatccct ttcgtttggc tggcctttgt
     1441 aggactgttg ggtggcacgg cgttcaagat cgtgcagatg gggagactgg ctagccggga
     1501 aaaaacagta tttcctacgt tggacgcgaa gcatggggcg cgctcggtgt tgcactggct
     1561 cttgcctttc ggcacgcgca acatgcgcct tcgccccttt ttcacggttg tatccttcgc
     1621 atttcacgcg tgtttgctga tcacgccgct tttcgtgatg ggacatgccg tgttgtggca
     1681 gcagtcttgg ggaataagtt ggtggagcct gcccgctcct gtcgcggact tcatgtcgtt
     1741 ggtggtggtg gctggaggac tgtttttcat tcttcgccgc atcgccgctc ctcaggttcg
     1801 taatgtaacg acctggtcgg actatgcgat tgttcttctg gtcatcgcac cttttgtgac
     1861 gggcttcgtt gcgcatcaag ggtggctgcc ttccaagcat gcgatcgcgt tgcacatcgc
     1921 ttccggaatt gcctggttgc tggccattcc cttcacacga ctcgcgcata tgttctggtt
     1981 cgttttctct cgggcctaca tgggtagcga gtttggtgcc gtccgtaacg ccagggactg
     2041 gtgaaaagga atcccgacca tgtctgatcc caccgatatg atgattgctg acgcgggcat
     2101 cgacgaaggc atcaaaaagc ttactcccga gcgtatcgag cagaccattc ggcacgtgtt
     2161 ggacaacgaa tccgcggcac gaatgaaggt atatctcgag acctgcgtgc attgtgggct
     2221 ttgcgcggaa gcttgtcata cctacaactc acgggaccga gatccggact acgcccctgt
     2281 ggccaaagtc aaaaataccc tttgggaaat ggtcaaacgc aagggcaagg tggacggccc
     2341 ctttttgaaa aacgcggcac gcatcgcctt caccgaatgc ggcgcgtgtc gtcgatgctc
     2401 catgtattgc cccttcggca tcgacatcgc gtatcaaatc atgaccgtgc gacgcatctg
     2461 cagcttgctg ggcctcgtcc cgcagtactt gcaggacacg accaacagcc atgcggtgac
     2521 gtcgaatcag atgtgggttc agccagacga ttggattgat acgttgatgt ggcaagagga
     2581 agaggcgatg tccgaattgt cgggcgcacg cattcctttg gacaaggaag gagccgacgt
     2641 tttttattcg gtcatcgctc cggagccgaa gattttggcg cagctcatcg gcaacatggc
     2701 cgtgatcatg aaagcggcaa acatcgattg gacgatgccg agcgccgatg ggtgggacaa
     2761 cagcaacatg gccatgtact cgggagattt cgaggtcatg ggacgtgtgg agcgagcgca
     2821 ttgggagcga gcggctcagc ttcgcgtcaa gcgtatcgtc atgggtgagt gcgggcatgc
     2881 ctaccgcggt gcggtgtacg acggtcccaa gtggcttgca tggaagcagc cgcccatccc
     2941 gatggtgcat gcggtcgagt tttattacga actcatcaag agcggaaagc tcgtcatcaa
     3001 gcggaaaatc gaagaacccg tgacggtgca ggacccttgc aacatcgtgc gagggcgcgg
     3061 gctgcacgac aagctccgct atgtgatcaa ggcgttgtgc gaggacttcc gggatatgga
     3121 gccgcgtcat gagcacaatt actgctgcgc ggcgggtggt ggtgtcatca actgcggccc
     3181 cccttggaaa ctgtcacgga tgaaaagcat ccgatgcaaa gccgatcaac tcgcggccac
     3241 gaaagccagc ctcatcatca ctccctgcca caattgccac agtggcatcg aggatatcat
     3301 cggtcactac aagctcggaa tgcacgtcaa gttcatcaat gagctgttgg tcaaggccat
     3361 cgatctcgag tcgtgaggag acagggaatc atgcgcatct ccagggcact gactgcgaat
     3421 tggatggtag tggttgtgat cgcttgcctc gcggtccttg gctgttcgga caaggacaaa
     3481 ccgatccttg gcccttccac catcgttctt cgactttctc ctcaatgggg ggcgttggat
     3541 cggccagcgg ttgacttcga tcacgccaag cacacggatg ctctcggccc caagtcgtgc
     3601 gtgagctgtc acacacaaga tagcgcgggc gtgatgaaat acgcatgggc cgctaccgct
     3661 gcttccgcca aagaagcaat gaataccttt cacgatggct gtttgaactg tcatcaggat
     3721 cgtgcttcga aaaaacaaaa aaccggcccg gtcgcttgcg gtagctgtca cgcccacgcg
     3781 cccggaacgt cgcaagaaca agcccatctg cgattcgatc aaagcctgca tcaccgccat
     3841 gaaaaagcgg aagatggtaa gtgcgagacc tgtcatcacg tatgggacaa ggcgcaagga
     3901 aaattccttc cgaaaaaagg tgaagaaagc gcttgtgccg attgccacgg cgccaaagac
     3961 gaaggacggg tcttgtcctt gcaacacgcc tcccacgacc gatgcgtgca gtgccatttg
     4021 caacgcgagg aaaagcaaca accgactggc cccaccttgt gcgttggttg tcacgatgcc
     4081 gaatcgatcg ccgcgatcaa gcgcgtcgat cctgtgcctc ggttgatgcg gggacaaccg
     4141 aatcacacct ggattcacgc cgatggcgct acggcagcgt atgttccttt cgatcatgcg
     4201 cagcatgagg ccgccacctc ccgttgttct tcatgccacc acaaaacgat gcgggcctgt
     4261 aaggattgcc acaccctcac acccacgagc gaaggcgggt acgtatctct ggaacgcgcc
     4321 taccacgagc cctcttccac gtttagctgc gtgggatgcc ataaaaaaga aacgcaacga
     4381 cccggctgtg tgggatgcca tcaaacgatt cccggcgagc cgagggaagc ttcttgcacg
     4441 acgtgtcacg cgggtccgac gacgaaagcg atgaaagcgg accttcccga gccaacgctt
     4501 gcgactgcaa atctcgaaga tctgccgccc acgtccgcgg ccttccccgc gactctccgt
     4561 ctcgaatact tccccggtgg tgattatggc cccgcggatc ttccccacaa aagaatcgtc
     4621 gagcacctgg atcgtgaggt cagaaaagac aagatggcca cacgtttcca cggcaccatg
     4681 gaaacgctat gcgcgggatg tcatcaccat tcccccgtcg gtgttcaacc acccaaatgc
     4741 gtgagttgcc accccgccga tgctgatctc agccgagatc gcccgggcct caaagtggct
     4801 ctgcaccgtc aatgcatctt gtgtcatcaa cccatgggga tcaaagccgg gtgcgtcgat
     4861 tgccacgccg aaaacaagga gcagaaacca tgagcgtctc gcgcagagga ttcctcaccg
     4921 gtcttgccac gggcgcgggc atgctcaccg cacacaaggc caacgccgcg ggagtgcagc
     4981 aattcacagg gtatcagggc cgtttcggac tgctgcacga taccacctta tgtgtgggct
     5041 gccgctcctg cgaaaaagca tgcaaagaag tcaatgggca ccccccgccg gaccgaccca
     5101 tcgatgacaa gaccgtgttc gaccgtcatc gacgaatcac gcccacggag tttacggtcg
     5161 tcaatcgata caagcccgcc gaggggcgta cgcccccggt gtaccgcaaa caccagtgca
     5221 tgcattgcaa tgaaccgtgc tgtgcttcgg tttgcctggt ccacgccttc gaaaagactc
     5281 ctgaaggccc cgtgctttac gatccggatg tttgcctggg ctgtcgatac tgcgtgatgg
     5341 cctgccccta tcatgcactt tcttacgagt acgacagcgc tttcgatccc aaagtgatgc
     5401 gttgcacgat gtgttatccg cgcatcaaag a
//
LOCUS       DAOSOR010000408         5419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15206_length_5419_cov_4.986764, whole genome shotgun sequence.
ACCESSION   DAOSOR010000408 DAOSOR010000000
VERSION     DAOSOR010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15206_length_5419_cov_4.986764"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4849)
                     /locus_tag="PK710_18360"
     CDS             complement(<1..4849)
                     /locus_tag="PK710_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11746.1"
                     /translation="MRDSNVSDALDRIMTGEKSTRSQGFTLCPHWLDRLMGRADAFEE
                     PVALPIVDGSVAGFAKVQAQALKAHLRGDRNGAKNACWWLASQLADADRHLAEALGYG
                     REALELGGEESWREQISRWLERVGAPREAASVLAPNVHVAAAASIAAKLLMRMARLQI
                     RGGALQEAMENLLEAATVWPTSADALELAGELAAVQGESTATTMWLEAADRHDQNGNR
                     ARAFEARRRAFALSPASAQACDALVDTLEQSGRFQAADETRLLHANAIDGSASRAVHA
                     IRLSRALEAEDSARALAVIVQGRLETVIAGSVAAQVDEALTQAGLHDAVTARWEWAAS
                     TEQGTSKARKLLDIAGIYLGRLASPDRALDAWIEAVAADPGCGEARASLRTHARAMRD
                     DTPLVEALIRAVQANPHGPAAIGCYRELATLAEERLSDPSLAHWALTELLIRMDDPQL
                     ESTRAALVPRIKLQDNALASAATAVEQGNTEAKLEGWRRMASILRGRPQEIQRYIEVL
                     THLVRLDREQSRWWLDLERATVRCGRQDHFERMVLERLEDPIAPNEKLHWLVTLMTHA
                     WRRGDPQSALSLAQRLTETLPGMRASHAHAWIAASATGDEELRALALEHLAPNLSASV
                     RATMLSLAATQWEALGNQEQAQRLMWQAKEADEGAPELLRLIASHAARSKDPALLRSL
                     EKLASIFVPTRENHATMTSLLSHAGNGDLAHAWARRWIELCPWDPEVAEQLLQTGVAS
                     TTEMHAAAILRVAACVFPTSDMERMLCGGLAELLSRDVHRAAQVAAELADMWGSHSEV
                     FRETLLAVALEAQDLRREAKIVERWLAHTTAPRQELLRLAKIYGDLDDREGQALALVR
                     AARASIEPHEILARLEPMQESISGDAALWILEARARCLAASGDTEGSALAWLHLGSAR
                     WDHAHDPVGAIEAWLEAMGHDALAFARLSESLAQRTTAEEVSDAFQDYALACTDPVRR
                     ACAFVVAAARAIGLERWTEAADLALQALREDPRRTDALTLIEVASGQGANHQAIDEAH
                     RLAAAGAKGRFGKRASHLRAARVLEARGFSEMALTHAIAAFEADPMQGSALTIMQRLA
                     QQENVADVVDCLCNVAENTQADQRALWWMQAATIASNHAQHQRRALDLGLRALLESPS
                     VEAMELIGRVLESLLRVDKEESSIFELRLQRALKALAPKLEGPIGARIAVSAATISVI
                     ILNAYSLALQWARAALACSGDIDEYDRLVALVPQLVMDKEGCGLFIQELIQRVRDRSG
                     TTGPSLMAFAHRLSEALGDGEWVRALRELEAETASDSSQPMDPFADLADAISVDSTPP
                     EPTKSQPEQDPSPKEAISDSAVSSRDGAMPTGQTDRLALADELVERGQEREAIEVLER
                     MGADTPNAQVDRRLRERYEALGLHERLVALLERMASQLDDPAEKVPLLVQVGALRDKL
                     GDKEQARAMWNLVVEVAPETREAWLRLEQDAMERGEWELLAHLLQRHSTVTPSASEAQ
                     ALQLRRVQLLDRSLMMSERAQGELDELIARGAANSAMLVYRAELAERLEGKLSAAGYW
                     VRAAMLADDRSEAVPLLCGAARAYLDLGVYDKAQQALEQIGDVRTEQVLTLKLEAELG
                     DSGPTA"
     gene            complement(4889..>5419)
                     /locus_tag="PK710_18365"
     CDS             complement(4889..>5419)
                     /locus_tag="PK710_18365"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOT11747.1"
                     /translation="EVFPASDAQEARARAAYLSPFVAWDEASRVRALKTMQSVYELFV
                     ERVAEGRHLEPQRIAAFAEGRIFAAREGLELGMVDEIGGLQDAIAYALRVGGLEPTGA
                     VRWVEQGSGLEKLLQLDPEDDVSAATGPMARNWVLRPILASTSSVEWLPYAEAWAPWI
                     QGERMLVAVPFAILIR"
BASE COUNT         1070 a         1813 c         1457 g         1079 t
ORIGIN      
        1 gagccgtcgg cccggaatcg cccagttccg cttccaactt caacgtcaat acctgttcgg
       61 ttctcacatc gccgatctgt tccagcgctt gttgtgcttt gtcatacacg cccagatcca
      121 agtacgcccg cgctgccccg cacaatagtg gcaccgcctc ggaacgatcg tccgccagca
      181 tcgcggcacg cacccaatag cccgccgccg agagcttgcc ttccaatcgc tccgccaact
      241 ccgcccgata gaccaacatc gccgaattgg cagctcccct cgcgatcaac tcgtcgagtt
      301 ccccttgcgc tcgttcagac atcatcaacg agcgatccag caattgcaca cgacgcaatt
      361 gcaaggcttg cgcttccgat gccgatggcg tcaccgtcga atggcgttgc agaagatgag
      421 cgagaagctc ccactcacct cgctccatgg catcttgctc gagccgtagc cacgcctccc
      481 gtgtttccgg tgcaacctcg acaaccagat tccacatcgc acgcgcctgc tctttgtccc
      541 ccaacttgtc tcgaagcgcg cccacctgca caagcaaagg caccttttcg gcagggtcgt
      601 cgagctgact cgccatgcgc tccaataacg ccaccaaccg ctcgtgaagc cccaacgctt
      661 cataccgctc ccgaagccgt cgatccacct gcgcattcgg ggtgtccgca cccatcctct
      721 ccaatacctc gatcgcttct cgctcctgcc cgcgctcgac caactcgtcc gccaaggcaa
      781 gcctatccgt ctgacccgtc ggcatcgccc catccctcga cgacaccgcg ctgtccgaga
      841 tggcctcctt tgggcttggg tcctgttcag gttgcgattt cgtgggctcc ggcggagtcg
      901 agtcgacgga tatggcatcg gccaaatccg caaatggatc catcggttgc gagctatccg
      961 aagcagtctc ggcttccaac tccctcaacg cacgaaccca ttcgccgtcg ccgagcgcct
     1021 ccgataaacg atgagcaaaa gccatcaacg acggtcccgt cgtccctgaa cgatcccgaa
     1081 cacgttgaat cagttcctgg atgaacaggc cacatccttc cttgtccatc accagttgag
     1141 gtacgagcgc aaccaaccgg tcgtactcgt cgatatcgcc agaacaagcg agagccgcgc
     1201 gcgcccattg cagggcgaga ctgtaagcgt tcaatattat tactgaaatc gttgctgcgc
     1261 tcacggcgat acgggcgccg atgggccctt cgagcttggg cgccaacgct ttgagcgcac
     1321 gctgcaacct caattcgaag atgctggatt cttctttgtc gacgcgaagc aacgattcca
     1381 aaacccgccc gatcaactcc atcgcttcca cgctcgggga ttcgagaagt gctcgtaagc
     1441 ctaggtccag cgcgcgacgt tgatgctgtg catggttcga tgcgatggtt gccgcttgca
     1501 tccaccatag ggctcgttgg tccgcttgtg tgttttccgc gacgttgcac aagcagtcca
     1561 ccacgtccgc gacattttct tgttgcgcaa gacgttgcat tatcgtgagc gcggagccct
     1621 gcatcgggtc ggcttcgaag gccgcgatgg catgggtgag ggccatctcc gaaaaaccac
     1681 gagcttcaag cactcgcgcc gcgcgaagat gcgatgcccg ttttccaaaa cgccccttcg
     1741 ccccggcagc ggcaagtcga tgcgcttcat cgatcgcttg atgattcgcc ccctgcccgc
     1801 tcgccacttc gatcaacgtt agcgcatcgg tgcgacgcgg gtcctctcgc aaagcctgca
     1861 atgctaagtc ggcggcttcc gtccaccgtt cgagcccgat cgcgcgagct gcagcaacga
     1921 cgaaagcaca ggctctacgc acgggatcgg tgcatgctag cgcgtaatcc tggaatgcat
     1981 cactgacctc ttcggcggtg gttcgttgag cgagggactc ggacagccgc gcgaaggcga
     2041 gcgcatcatg acccatggct tcgagccatg cttcgatggc tcccaccgga tcatgggcat
     2101 gatcccacct tgcggaaccc aggtgcagcc aagcgagtgc cgagccttcg gtatctcctg
     2161 atgctgccaa acatcgcgca cgagcttcga gaatccaaag cgctgcatca ccggatatcg
     2221 attcctgcat cggttccaga cgagccaaaa tttcatgcgg ttcgatcgag gctcgagccg
     2281 cacgcacgag agccagcgct tgtccttccc tatcgtccag atccccgtaa atcttcgcaa
     2341 gtcggagcag ctcttgccgt ggtgccgtgg tatgcgccaa ccatcgctcc acaatcttgg
     2401 cttcccttcg caggtcttgg gcctccaaag ccaccgcgag cagtgtttcc cgaaatactt
     2461 ccgaatggga accccacata tcggcaagct ccgcggcaac ctgtgcagca cgatgcacat
     2521 cgcgcgaaag caattcggcg agccccccgc acaacatccg ctccatgtcc gaagtaggaa
     2581 aaacacaggc tgccacgcgc aggatcgccg ccgcatgcat ctccgtcgtc gatgcgaccc
     2641 ccgtttgcaa caattgctcg gcaacctcag gatcccaggg acataactcg atccaacgac
     2701 gagcccaggc gtgagccaaa tccccgttgc ccgcgtgaga cagaagcgat gtcatggtgg
     2761 catggttctc acgggtggga acgaagatgg aggcaagctt ttctagggat cgaagcaggg
     2821 ccggatcctt ggagcgagct gcatgcgatg cgatgagacg cagtagctcg ggggcccctt
     2881 catcggcttc tttggcctgc cacatcaacc gttgcgcttg ctcctggttt cccaatgcct
     2941 cccattgggt tgcggcaaga gaaagcatcg tggctcgtac cgaggccgaa agattcggcg
     3001 ccaaatgctc caacgccagc gcccgaagct cctcatcgcc tgtcgccgat gcagcaatcc
     3061 acgcatgcgc atgcgacgcc ctcatgccgg gcaaggtttc tgtgagccgc tgtgccaatg
     3121 acaatgctga ttgtggatca cctcttcgcc acgcatgggt catgagcgtg acgagccaat
     3181 gcagtttttc attgggcgcg atcgggtctt cgagccgttc gagaaccatt cgctcgaaat
     3241 ggtcctggcg cccgcagcga accgttgctc gctccaaatc gagccaccat cggctttgct
     3301 ctcgatccaa acgaacgaga tgcgtcaata cctcgatgta tcgttgaatc tcttggggac
     3361 ggccccgaag aatcgacgcc atgcgtcgcc acccttcgag cttggcctcc gtatttcctt
     3421 gttcgacggc tgtagccgcc gaggcaagag cattgtcttg cagtttgatc cgcgggacga
     3481 gcgctgctcg agtgctttcg agttgcgggt cgtccatgcg gatgagcaat tccgtcaacg
     3541 cccaatgcgc caaggaaggg tcggagagac gttcttccgc gagcgtagcc agttctcgat
     3601 aacagccgat agccgctggc ccatggggat tggcctggac cgcacggatc aaggcttcta
     3661 ccaacggcgt atcatcacgc atcgctcgcg cgtgcgtgcg aagggacgct ctagcctccc
     3721 cacatccggg gtcggcggcc accgcctcga tccaggcgtc gagagcccgg tccggactcg
     3781 cgagccgtcc aagataaatc cctgcaatat caagtagttt ccttgctttc gaggtaccct
     3841 gttccgtcga agcggcccat tcccagcgcg ccgtaaccgc gtcgtgaaga cctgcctgcg
     3901 tcaacgcttc atccacctgc gcggccacag acccggcgat caccgtctcg agcctgcctt
     3961 gaacgatcac cgcaagagcg cgggcagaat cctccgcttc gagagcccga gaaagacgaa
     4021 tggcatgcac tgcgcggcta gcagagccat cgatggcgtt ggcgtggagg agacgagtct
     4081 catcggcggc ttggaaacgc ccggattgct cgagggtatc gacgagcgcg tcacacgctt
     4141 gggcgcttgc tggtgatagc gcaaaggctc gacgtcgcgc ttcgaaagct cgggctcgat
     4201 tgccgttttg atcgtgacga tcggctgctt cgagccacat cgtggtggct gtcgattcgc
     4261 cttgtacggc ggcaagctca ccagccaact cgagggcatc cgcactggtc ggccatacgg
     4321 ttgctgcctc cagcagattt tccatggctt cttggagcgc tcccccccga atttgcagcc
     4381 ttgccatgcg catcaacaat ttggccgcga tgcttgcggc ggctgcgacg tgaacgtttg
     4441 gcgccaggac cgatgccgct tccctcggcg ctccgactcg ctcgagccat cggctgatct
     4501 gctctctcca cgactcttcg ccccccaact cgagcgcttc acgaccgtat ccgagcgctt
     4561 ccgccaagtg ccggtccgcg tccgcaagct gcgaggcaag ccaccaacac gcgtttttgg
     4621 ccccatttcg gtctccccgc aggtgggctt tcaacgcttg ggcttgtact ttggcaaacc
     4681 ccgccaccga cccgtccacg atgggcaatg ccactggctc ttcgaaggca tccgcacgac
     4741 ccatcaatcg gtcgagccaa tggggacata gcgtgaaacc ctgtgatcga gtggattttt
     4801 cacccgtcat gatcctgtcc aacgcgtcgg aaacgttcga atcccgcatg ccttcctgac
     4861 ctaccgccca acacggtccg tccaggggct agcgtatcaa aatcgcgaaa ggaactgcta
     4921 caagcatgcg ctctccctgt atccagggag cccaagcctc cgcataaggc aaccattcga
     4981 cggaggacgt agaagcaagg atggggcgta aaacccagtt tctggccatc ggtcccgtcg
     5041 ccgcggaaac gtcatcctcc gggtcgagtt gaaggagctt ttccaatccg cttccttgct
     5101 ctacccaacg aaccgccccc gtgggctcca atcctcccac acgaagtgca taagcgatgg
     5161 cgtcttgcaa gcctccgatt tcgtccacca tgcccaattc gagcccttcg cgcgcggcga
     5221 aaattcgtcc ttcggcgaat gcagcgattc gttgcggctc caaatgacgc ccttcggcga
     5281 cgcgctccac gaataattca taaacgctct gcatcgtttt caacgctcgg actcgggagg
     5341 cctcgtccca agccacgaac ggcgaaagat aagccgcccg cgcacgagcc tcctgggcat
     5401 cgctagcggg gaagacctc
//
LOCUS       DAOSOR010000409         5414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15222_length_5414_cov_5.184923, whole genome shotgun sequence.
ACCESSION   DAOSOR010000409 DAOSOR010000000
VERSION     DAOSOR010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15222_length_5414_cov_5.184923"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /gene="sfsA"
                     /locus_tag="PK710_18370"
     CDS             <1..602
                     /gene="sfsA"
                     /locus_tag="PK710_18370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HOT11748.1"
                     /translation="GSRCLLLPSHNPKRRLAYTLEAVRVGKRWVGTHPARANTIGLEA
                     IMAGLVSAVPQIASARAEVAYGTSSRADWVLQTRHGDVWTVEIKSVSLAEDQVALFPD
                     AVTERGRKHLDELCRVVKKGGNALMLFVATRDDVKLFRPALSIDPAYARRLAQAAHEG
                     VLVRAVTSRVTRTQMVPLREIPVDLEGAAATASPSKTRL"
     gene            complement(756..1160)
                     /locus_tag="PK710_18375"
     CDS             complement(756..1160)
                     /locus_tag="PK710_18375"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOT11749.1"
                     /translation="MTDKQELQLREKKELKQEGEATREGPHFRPEVDIFEREDALVMV
                     ADVPGSSNEQIELDLQDNSLNLTATVSGVDAKWKPVYLEYEIGHYIRQFRIGQIVDMA
                     KISAQVKDGVLVVTLPKIEKAMPRKIEVKSEQ"
     gene            complement(1178..1600)
                     /locus_tag="PK710_18380"
     CDS             complement(1178..1600)
                     /locus_tag="PK710_18380"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOT11750.1"
                     /translation="MPYRYWGDPITMLNELRKRQLQSMREAEGHEFAGVYPAVNIYDD
                     GDAFMVRAELPGIDKAKLDITTKGNRLSIRGERSCSQLAADVAFHRRERDMGTFHRVV
                     SLPEHVDASKTTATYKNGILEIVCPRTEAAKAHRVEIA"
     gene            complement(1739..3151)
                     /locus_tag="PK710_18385"
     CDS             complement(1739..3151)
                     /locus_tag="PK710_18385"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOT11751.1"
                     /translation="MTFLFPILLAIAAVLLFGVLGDVVFQRTGISSTLWLIVCGLLLG
                     PVLGLFPREQAWELGPVLAATALCVLLDEIRDCLSWSTLRRDLALAGPRAAISFGVSA
                     LSVTLVAGLAHEAGLAPEAFGWKHALLLGAVLAAPCAWIITPILQPIAPSAARIIHQE
                     SVLGASAAILWCSILIALFDPRAETALEASIQLARSLLLGVGIGALATALWVGFLFLL
                     RSPAHHRLSTLAMSLIVFATATQIGSSGAIAVLVFTLTLANVKAVFPRLPWARMLEVP
                     ENPPGRQPLISFFVASMFFVFTGLVLGPPWDLMVLSLLLCVILFPPRILAVLVGGHFA
                     RDSASGPSDRAFASVAIARGILAGALSLLPFLVSMEHSEYVSIPAFTVMAGSITIFTA
                     AFAILSIRENKRVINSIAQPHVQILHDKQSQQAFRSASEEPIPPTTRSPSRDQGPRTS
                     IDKPTRPLPPPVPPPRRHEK"
     gene            complement(3261..4124)
                     /locus_tag="PK710_18390"
     CDS             complement(3261..4124)
                     /locus_tag="PK710_18390"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HOT11752.1"
                     /translation="MTHVTCRDLARSLSAFVDGELDPTLALELQLHIDGCEACEARVK
                     FARASRTSVRNAVKSTKASDSFRLRVATTVAQAAEAEKHGDIMVSSGLLSWKIAGPLA
                     AAAAFVLVWASASKLQERNLDKTTSSSPSTSQASMIPVDTLVDTLVDQHANPLPPETS
                     SPSEIAQFDRFIGVPIRTVPERGFQGQGHLLGARMVPLQEQRAAMFQYVMGNGRRISM
                     YIYNPQKIRLSNSPILREIPVRNNAGPVYVGCVRGYSVAVTDRRGVGYAMASDLDESE
                     SSRLLLAVGDH"
     gene            complement(4121..4783)
                     /locus_tag="PK710_18395"
     CDS             complement(4121..4783)
                     /locus_tag="PK710_18395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT11753.1"
                     /translation="MSFFPFLNRSRVRQDTFEDDVLSHVDSLFATALRMTRSQADAED
                     LVQDTLVKAVRGRALFEDGTNLKAWLFKILTNTFINRYRRGSLEREIISGADARPLSD
                     GWMSAASMRQMCDAESQAFRPIIEKEVNKALEQLPDEFRIPVLLSDVEDFSYREISEI
                     MGCPIGTVMSRLYRGRRLLQIALHDHALAMGLVKGEAVREDESNMVNLESYRTRKQGV
                     GG"
     gene            complement(4832..>5414)
                     /locus_tag="PK710_18400"
     CDS             complement(4832..>5414)
                     /locus_tag="PK710_18400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HOT11754.1"
                     /translation="QIAEMVRHGEDVQIIDNTTKEDKTEVTLALAISEELKAKPRSVP
                     LGTLRNLIQERGEKILTSLRDGPIGRLIPEGESEAAEKKEHEVSEEKLKEREKERQER
                     TGLAELMASSRHTLDQWQHAIDERIRAVLPSFSLFLDIQAEMRRLTQRVQFLEERFGQ
                     KQRASEGGKEPAESTMESGKIAPPPGQDATPKE"
BASE COUNT         1209 a         1495 c         1555 g         1155 t
ORIGIN      
        1 caggctcccg ctgcttattg cttccctccc ataatcccaa acgccgtttg gcctataccc
       61 ttgaagcggt tcgcgttgga aaacgctggg tgggaaccca cccggcccgc gccaatacca
      121 ttgggctaga agccatcatg gcgggactcg tatcagcagt accccaaatc gcttcggctc
      181 gcgccgaggt tgcgtatggg acgtccagtc gagccgattg ggtcttgcaa acacggcacg
      241 gcgacgtttg gaccgtcgag atcaaaagcg tttcgctagc ggaagaccag gtcgcgctat
      301 ttcccgatgc ggtcaccgaa cgtggccgaa aacacctgga tgaactgtgc cgtgtcgtga
      361 aaaaaggagg aaatgccctg atgctttttg tggcaacccg cgatgacgtc aaacttttcc
      421 gtccggcgct gtcgatcgat cccgcttacg cccgtcggct cgcccaagcc gctcacgaag
      481 gcgtgctggt tcgtgcggta actagccgcg tcacacgcac gcaaatggtg ccgcttcgag
      541 agattcccgt cgatctcgaa ggggccgccg ccaccgcatc accaagcaaa acaaggttat
      601 gactcatcaa catcgggcgt tggatacgcc cggtcgagag gtgacagccc ggtggaggga
      661 gtcggtcacg ttttgacggg aaggcatcgc ggaaactcat cgaccagcaa gccgtcgatc
      721 catgcctgcc gatcgatgag ccgctatggt gcaggtcatt gctcggactt gacttcgatc
      781 ttgcgaggca tggctttttc gattttgggc agcgtgacca cgagcacccc atctttgact
      841 tgagccgata ttttggccat atcgacgatt tgtccaatgc gaaattggcg gatgtaatgg
      901 cctatctcgt attcgaggta cacaggcttc catttggcat cgacaccgct gaccgtggcg
      961 gtgagattca aggaattgtc ctgcaagtcg agttcgatct gctcgttgga tgaacctggc
     1021 acatccgcaa ccatgaccaa ggcatcctcc cgttcgaaaa tatcgacttc gggtcggaaa
     1081 tgaggcccct cccgcgtcgc ttctccctct tgcttgagtt cctttttttc acgaagctga
     1141 agttcttgtt tgtcagtcat aaggcacctc ctcgctctca ggcgatctct acgcgatggg
     1201 ctttggctgc ctcggttcgc ggacagacga tctcgaggat gccgttcttg taggtagcgg
     1261 tcgttttgct tgcatcgacg tgttctggga gactgacgac ccgatgaaat gttcccatat
     1321 cacgctctcg tcggtgaaaa gctacatcgg cagcgagttg cgagcagctt cgctctccac
     1381 ggatcgatag acgatttcct ttggtcgtaa tgtcgagctt ggctttgtcg attcccggaa
     1441 gttcggctcg aaccatgaac gcatcgccat cgtcgtaaat gtttaccgct ggatacacgc
     1501 ccgcaaattc gtgtccctcg gcctctcgca tactctgcaa ttggcgtttg cgaagttcgt
     1561 tgagcatggt gatgggatcg ccccaatatc gataaggcat cgcattcctc cgtcgctgcg
     1621 ggtcccccag ggcccgcgat ggttggatag actgttcgtg attcgtctca atcacggagt
     1681 gtctgacgtt cgccatgagt aatcacccaa tgggaacggt caagggggtg acacgcttct
     1741 acttttcgtg gcgtcgagga ggaggtacgg gaggaggaag gggcctggtc ggtttgtcga
     1801 tgcttgtccg aggcccttga tcgcgactgg gtgagcgtgt ggtaggggga atgggttctt
     1861 ccgacgcgct gcggaatgcc tgctgtgatt gtttgtcgtg caggatttgg acgtgaggtt
     1921 gcgcgatgga gttgatgacg cgtttgtttt cgcgaatcga caggattgca aaggcggcag
     1981 tgaagatcgt gatgctgcct gccatcacgg tgaacgcggg tatcgagacg tattcggaat
     2041 gttccatgga taccaagaac ggaagcaaag aaagagcgcc ggcaaggata ccgcgggcaa
     2101 tggccacgga tgcaaacgcg cggtcggagg ggccagaggc cgaatcacgc gcgaaatgcc
     2161 ccccaacaag gacagccaaa atacgaggcg gaaacaggat aacgcaaagc aacaggctga
     2221 gtaccatgag atcccaagga ggtccgagaa ccagtcccgt gaatacgaag aacatggacg
     2281 caacaaaaaa cgaaatgagg ggctgacgcc caggcggatt ttcgggaact tcgagcatcc
     2341 ttgcccaagg aagacgcgga aacacggctt tcacgttggc aagggtgagc gtaaaaacga
     2401 gcacagcgat cgcgccactg gaaccgattt gggtcgccgt agcgaaaacg atcaggctca
     2461 tcgcaagggt cgagaggcga tgatgggccg gtgaacgaag aagaaaaaga aaaccaaccc
     2521 aaagggcggt cgcgagtgcc ccaatgccaa cacccaagag caatgaacga gcaagttgga
     2581 tggacgcctc cagcgctgtc tcggctcggg ggtcgaacag agcaataaga atggaacacc
     2641 acagaatggc cgcacttgct ccgagcacgg attcttggtg gatgattctc gctgctgacg
     2701 gtgcgattgg ctgaaggatg ggcgtgatga tccacgcgca aggggcggcc aagacggctc
     2761 caagcagcag cgcgtgcttc catccaaagg cttccggggc aagtccggct tcgtgggcca
     2821 agcccgccac gagcgtgacc gataatgccg aaacgccgaa agaaatcgct gcccgtggac
     2881 cagcaagagc gaggtcgcgt cggagcgtag accacgagag acaatcgcgt atctcatcga
     2941 gcaagacaca aagagccgtg gcggcgagaa ctggaccaag ctcccacgct tgctcacgtg
     3001 gaaatagacc cagcaccggg ccgagcaaaa ggccacaaac gatcaaccat agggtggacg
     3061 aaatacccgt gcgttgaaat acgacatctc ccaatacacc aaagagcagc accgcagcga
     3121 tggctaggag gatagggaaa agaaacgtca tcgggcgaag atcattggcg caagacagcc
     3181 agccgactcc tgcgtgtcat gccacgcacc ttccgtcaac tacagaatga tcaagctcga
     3241 acgcttgtca ggcaggtgaa tcaatggtcg ccaacagcca acaaaagcct ggaactctca
     3301 ctttcgtcca agtcgctcgc catcgcataa cccacaccgc gtcggtccgt aaccgctacg
     3361 gagtatccgc gaacacaacc gacatacacg gggcctgcgt tgttacggac gggaatttca
     3421 cgaagaatgg ggctattgct gaggcggatt ttttggggat tgtagatgta catactgatg
     3481 cgtcggccat tacccatgac atattgaaac atggcggcgc gttgttcttg cagcgggacc
     3541 attcgcgcgc cgagcaagtg tccttgacct tgaaaaccac gttccggaac cgtgcggata
     3601 ggaacgccga tgaaacggtc gaattgcgcg atttcggaag gactgctggt ctccggcggt
     3661 aagggattgg cgtgctggtc caccaacgtg tccaccaacg tgtccacggg gatcatggat
     3721 gcttggctgg tcgacggaga ggatgatgtt gttttgtcga ggtttcgttc ttggagtttg
     3781 gaagcggagg cccaaaccaa cacgaatgca gccgccgcgg cgagaggacc cgcgattttc
     3841 cacgaaagca gaccgctgct caccatgata tcgccgtgct tttcggcttc ggcggcctgc
     3901 gcaactgtcg tggcgacgcg taaccgaaaa gagtccgaag ccttggtcga cttgacggca
     3961 tttcgcacgc tcgtgcggct tgctcgcgca aacttcacac gggcttcgca ggcttcacat
     4021 ccatcgatat gaagttgcaa ctccagcgcc aacgtcggat cgagttcgcc gtccacgaac
     4081 gcactcaggc tgcgcgccag atcacgacac gtcacgtgcg tcatccgccc accccctgtt
     4141 tgcgagttcg ataagactcg agattcacca tgttggattc atcctcccgg accgcctctc
     4201 ctttcacgag tcccattgcc aaagcgtggt catgtaacgc gatttgaagc agccgacggc
     4261 ctcgatacag gcgtgacatc accgtaccaa tcggacaacc cataatttct gagatttccc
     4321 gataggaaaa gtcttcgacg tccgaaagta gcacggggat tcggaattcg tcgggcaatt
     4381 gttccaaggc tttgttgact tctttctcga tgatgggccg aaaagcctga gattcggcgt
     4441 cgcacatctg acgcatcgaa gccgcgctca tccaaccgtc ggacagaggg cgcgcatctg
     4501 caccactaat gatctcacgc tccaaactgc cgcgtcgata ccgattgatg aaagtattgg
     4561 tgaggatttt gaaaagccac gccttgaggt tcgtgccatc ttcaaataac gcgcgccctc
     4621 ggactgcttt gaccaaagtg tcttgcacca agtcctcggc gtccgcctgg ctcctggtca
     4681 ttcgcagcgc cgtggcgaaa agcgagtcga catgggaaag aacgtcgtcc tcgaaagtat
     4741 cttgcctgac gcggcttcgg tttaggaaag gaaagaatga cattgggatt ccgttgggtg
     4801 ttccatcgcc ccaaccgata ggtcccgtga gttattcctt cggggtggca tcttgtccag
     4861 gcggaggtgc gatttttccg gattccatgg tggactccgc aggttccttg cccccctcac
     4921 tggcccgctg cttctgcccg aagcgctctt cgaggaattg gacgcgttgt gtcaaacgac
     4981 gcatttccgc ctggatatcc aaaaacagcg agaaactagg caaaactgcg cgaattcgct
     5041 cgtcgatggc atgttgccat tggtcgaggg tgtgtcgaga actggccatc aattcggcga
     5101 gcccagtgcg ctcctgcctt tctttttcac gctctttcag cttttcctca gacacctcgt
     5161 gttctttttt ctctgctgcc tccgattcgc cttcgggaat caagcggcca atggggccgt
     5221 cgcgtagcga ggtgagaatt ttttctccac gctcttgaat gaggttacga agggtcccca
     5281 acggaacaga acgaggtttg gccttgagtt cttccgaaat cgcaagagcc agggtcacct
     5341 cggtcttgtc ttctttggtg gtgttgtcga tgatctgaac gtcttcgccg tgccgcacca
     5401 tctccgcgat ttgc
//
LOCUS       DAOSOR010000410         5412 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15234_length_5412_cov_3.863543, whole genome shotgun sequence.
ACCESSION   DAOSOR010000410 DAOSOR010000000
VERSION     DAOSOR010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15234_length_5412_cov_3.863543"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            743..1027
                     /locus_tag="PK710_18405"
     CDS             743..1027
                     /locus_tag="PK710_18405"
                     /inference="COORDINATES: protein motif:HMM:NF015927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HOT11755.1"
                     /translation="MGLMVNVMLAAQPGEVDALWSTAAIVTALKAFGASVVYSFLGLI
                     MFALAYKVLGFIFPFSIRKELEEDQNTAVGIVLGCMMLGLAIIIAAAIHG"
     gene            1034..1759
                     /locus_tag="PK710_18410"
     CDS             1034..1759
                     /locus_tag="PK710_18410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11756.1"
                     /translation="MSSAPLPHRNPVAAPRLPPPRHVALLYAVLVVLASTGLAYELSL
                     GAVESFLLGEPVSSFALIISGTMVALGLGSYLSSWIKTELELRFIDAAVATATVGGAS
                     APMLYVVFGLHGPFRFVLYAATLLIGTLVGLQLPLLLRILKRNERFADVVARGFALDY
                     AGGLAGSLAFSFVFMPSLGLIRTTLVLGCLNLVVAAYGIHLLGWKRKGMKLRGLTLVG
                     VGALLMTLGWFAPHFEAMTERPL"
     gene            2083..3006
                     /locus_tag="PK710_18415"
     CDS             2083..3006
                     /locus_tag="PK710_18415"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HOT11757.1"
                     /translation="MDTTLLYRSAVLNVANHRTVQHLVTRRAWRFARRFVAGTTIDEA
                     LTAIDALEKDGIHGILDLLGEMVTSQQEVLEFSQLIGQHVTSFASRPYPRYLSIKLTQ
                     IGLGLDFDLMIESAKTILSTAREHDTFVRIDMEDSPRVDDTLRAFRLLREQGFDNLGL
                     VLQSSLRRTESDLRSLLDLKPNLRIVKGAYKEPPTVAYQDKSTVDAAFVSLVKTNLAE
                     GNHTAIATHDERIINQLQSWNAEQGIPSERVEYQMLYGIRRDLQRRLAAEGHFVRAYV
                     PFGTQWYPYFSRRIAERPENAWFVAKALIAG"
     gene            complement(3013..3606)
                     /locus_tag="PK710_18420"
     CDS             complement(3013..3606)
                     /locus_tag="PK710_18420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11758.1"
                     /translation="MYGRSRQHWVLRRGQGALCLLVMLYSGVARAESFIERPDAHPAT
                     QVEAEPHLVFGWLDPPGIADGSGYGMGIRGTFELVDPGFIPRLNNTVGIGVGLDWLRY
                     HAEDCHRNEVGDWECEDDRARYVWVPVVLQWNFFLHEQWSVFGEPGVALRFQSPGNNK
                     FDPFVLYLGGRWRFSDQASLTMRIGYPVTTVGVSFFL"
     gene            complement(3725..4627)
                     /locus_tag="PK710_18425"
     CDS             complement(3725..4627)
                     /locus_tag="PK710_18425"
                     /inference="COORDINATES: protein motif:HMM:NF027464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HOT11759.1"
                     /translation="MAQPTPQVSESPRTVPTPSPAAPTSATSAVERTIGPVDLARYLW
                     HADSSLEPLEAVDDLLLRVNTVEGYTRVSLPDDSWGAWLRRLPLAAAGSPVRSFSGTV
                     ILPGDHENLAAVATLDIGKADLQQCADAVMRLHAEWDWANGVREITFQAASGMALPLA
                     RWEKGERIVPKGSSIMWVPAAAPSKDDHRSFRKYLDAVFAWANTVSLERQAKKVELDD
                     LQPGDFFILPGNPGHTVLVLDLAVDGSGQRMVLLGQSYMPAQNFHVLRPRRDTAWFSL
                     DPRAPGVQTPFWPKPFPWSSIRRL"
     gene            complement(4794..>5412)
                     /locus_tag="PK710_18430"
     CDS             complement(4794..>5412)
                     /locus_tag="PK710_18430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11760.1"
                     /translation="GPVPVHRDLGVLVRGLVLDDVLDYRLAVTDGVAPNAAGDYPRVV
                     GRLGVNLMDVEPDYFWKGTYLGSKSIVSFGFSFDIEPGVGGDTGNKTFYALAFDALVD
                     VPIGENGIVATVAGHHYGSGGTIPKGVGIWGDLGYRIKKIEPLVEVEYYQPSEEGNRR
                     IGLFGGANYWIKGHHANLKLQVGAVDVETKLDWATEVLMQGQVMF"
BASE COUNT         1117 a         1611 c         1460 g         1224 t
ORIGIN      
        1 gctcttttcc gtcgagcgtg acgtgggcgc ggcgtggctt tgcaccggag cccaaagcag
       61 ccagaccagc gcaaaaagca cgagcacacg cggtgaaatt cgcatcttcc atcctgtgct
      121 gacaaacgac aggcagacgc gtcaagcata attctctccc gccgagccat cttgcgcatc
      181 gattgccgtt ttccttgttg gagtacgcgc atggggctag cgcattttca tcctttgccg
      241 tcgaacggac gaccagattc tctccatcgg cgctagcact tccccctctt ccaacttggg
      301 ccgaacccat gctagggttt tggaatgccc acaatggagg tcggcgaaga gtggatagtc
      361 gatgctttcg ggtgtgagcc tcaccttctt cgtgacgctt cttgtctcca atcgatcgtg
      421 aaatccattc ttcgggacac atctttgcat tcgctccacg aagggtactg gcatacgttc
      481 gccggcgaag ggggcgtgac tggattggtc gcgttgcagg agtcacatct cgcagtgcat
      541 acgtaccccg agtatcgcat cgcaacattt agcctgtatt gctgtagaat cggatgtggt
      601 tggagttggg acgaaaagtt gaaaaaggcg ttaggagcaa cgacggtcac gatcgttcgt
      661 gtcgagcgcg gccggttgcc cgcgacgacc agttcgaatg gtatgcctac aaaaatggaa
      721 cactcacggg agggatgatg ccatggggct gatggtcaac gtcatgcttg ccgcacaacc
      781 aggggaagtc gatgccttgt ggtcgacggc cgccatcgtc acggctctca aagcctttgg
      841 tgcctcggta gtctacagct tccttggcct catcatgttt gccttggcct acaaggtatt
      901 ggggttcatc tttccgtttt cgattcgaaa agaattggag gaagatcaga acaccgcggt
      961 gggtatcgtg ttgggctgca tgatgctggg tcttgccatc atcatcgccg ccgctatcca
     1021 cggttgaacc gccttgtcct ccgctcccct gccccaccga aacccagtag ctgctcctcg
     1081 ccttccgccc cctcgccacg tcgccctttt gtatgccgtg ctcgtcgttt tggcatccac
     1141 cggcttggcg tatgagctat cgctcggtgc ggtagaaagc tttttgctcg gtgaacccgt
     1201 ttcgagcttc gcgttgatca tcagcggcac catggtagcg ctgggactgg gttcttatct
     1261 gtcgagttgg atcaaaaccg agttggaact tcggttcatc gatgcagcgg tcgcgacagc
     1321 caccgtgggg ggcgcttccg caccgatgct ttacgttgta ttcggcctcc acgggccgtt
     1381 tcgattcgtc ctgtatgccg caacgctgct gattgggacc ctcgtcggcc tacaactccc
     1441 attgctgctg cggattctca aacgcaacga acgattcgcg gatgtcgtgg cacgaggctt
     1501 cgccttggat tacgccggtg gcctcgccgg ttcgctcgct ttttctttcg tgttcatgcc
     1561 atcgctcgga ctcattcgaa ccacgctcgt tcttggatgc ctcaacctgg tcgtcgcagc
     1621 gtatggaatc cacctgttgg ggtggaagag aaaaggcatg aaattacgcg gccttacact
     1681 cgttggggtc ggcgctttgc tgatgactct ggggtggttt gccccccact tcgaagcgat
     1741 gaccgagcgc cctctataat aaactataat catttcgttt agttatactc acgccgacct
     1801 gtcgcaagca ccgtatcgcc atgtgtccct gctcggctcg tgcccgaccc gtcccctcat
     1861 tttccctcga gcatttcctt cgacatcctg gggacaacag ctcatatccc ctgaaatcgt
     1921 ggatgttgac cattgcctta tccctgacgg cagccccccc atccggctga cccatccgac
     1981 ccatgatgtt gggcaccttg ctctcgcccc ggctctggca aaacggaccg tgcctcgttc
     2041 tgccctcttg catcgtgggt gtccggctct agatcgcctg ccatggatac caccctcctg
     2101 taccgttccg ccgtactcaa cgtcgcgaat catcgaaccg ttcagcatct cgtgactcgc
     2161 cgggcgtggc gttttgcccg acgattcgta gcaggaacga ccatcgacga ggcgctgacg
     2221 gccatcgatg ctctcgaaaa agatggtatt cacggtattt tggacctgct gggggagatg
     2281 gtgacatcac aacaggaggt gcttgagttc tcccaattga ttgggcagca cgtcacctct
     2341 ttcgcttccc gaccttaccc ccggtactta tccatcaaat tgacgcaaat cgggctcggc
     2401 ctcgatttcg atttgatgat cgaatcggcc aaaaccatcc tatccacggc tcgggaacac
     2461 gacactttcg tgcgaatcga catggaggat tctccccgcg tggacgatac tcttcgcgcg
     2521 tttcggctcc ttcgcgagca aggcttcgac aatctcgggc ttgtgttgca gtcgagtctt
     2581 cgtcgtacgg aatcggattt gcgctccctt ctcgatctga aacccaacct gaggatcgtc
     2641 aaaggcgcgt acaaagagcc gcccaccgtc gcgtaccaag acaaatccac ggtggatgcc
     2701 gcgtttgtct ccctggtcaa aaccaacctt gccgagggca atcataccgc gattgccacg
     2761 cacgacgagc ggatcatcaa ccagcttcaa tcgtggaacg ccgagcaggg catcccgtcc
     2821 gagcgagtcg aataccaaat gctttacggc atccgtcgcg atttgcaacg tcgccttgcc
     2881 gccgaaggtc atttcgtccg cgcctatgtg ccgttcggca cccaatggta tccctatttt
     2941 tctcgccgaa tcgccgaacg ccccgaaaac gcctggttcg ttgccaaagc gttgattgcg
     3001 ggttgatggc gttcacaaga aaaaagaaac gcccacggtg gtgacgggat aaccaattcg
     3061 catggtcaga gaagcttgat cggaaaaacg ccaacgccct cccaggtaca aaacgaacgg
     3121 atcaaactta ttgttgccgg gtgattggaa gcgcagggcc acgccgggct ccccgaatac
     3181 cgaccattgt tcgtgcaaga aaaagttcca ttgcagcacg acgggaaccc atacgtatcg
     3241 cgcccgatcg tcttcgcatt cccagtcgcc gacttcattt cgatggcagt cctcggcgtg
     3301 ataacgaagc cagtcgagtc caacgcctat ccccaccgta ttgttcaacc gaggaatgaa
     3361 tccaggatcc acgagttcga aggtcccgcg aattcccatc ccatatccgc tcccatcggc
     3421 tatccccggg gggtcgagcc agccgaatac gagatgcggc tccgcttcga cctgcgtagc
     3481 aggatgcgcg tcgggtcgtt caatgaacga ttccgctctt gctaccccgg aatacaacat
     3541 caccaataga cataacgccc cttgccctcg tcgtaacacc caatgctgtc ttgacctacc
     3601 atacatgagt atcttgactc ctcgatcgag gagatggtgt aaacaaaccc cggttcgacg
     3661 gccaagtccc gcggatatcc tgccgccaaa tcagcctatc caattgaaaa taaacagatt
     3721 ttttctacag ccgtcggatg gatgaccagg ggaagggctt gggccaaaag ggggtttgca
     3781 cacctggtgc ccttgggtcg agggagaacc aagccgtgtc ccgtcgtggc cgcagcacgt
     3841 gaaagttctg cgccggcata tagctttgcc cgagcaaaac catgcgctga ccacttccat
     3901 cgaccgccaa gtcgagcaca aggacagtgt gtccaggatt tccgggtaga ataaaaaaat
     3961 cccccggctg gagatcgtcc aattcaactt ttttcgcctg tctttcgagg gataccgtgt
     4021 tggcccaggc aaatacggca tccaggtatt ttcgaaacga ccgatgatcg tctttgctcg
     4081 gtgccgcggc gggaacccac atgatggatg aaccttttgg tacgattcgc tccccttttt
     4141 cccaccgggc caaaggcaaa gccattccac tggcggcttg aaacgtgatt tccctcactc
     4201 cgttggccca atcccattcc gcatgaagac gcatgacggc gtcggcgcat tgctgcaagt
     4261 ctgctttgcc gatgtcgagg gtcgccacgg cggccaggtt ttcatggtct ccgggcagga
     4321 tcacggttcc tgaaaaggag cgtacggggc tgccggccgc ggcgagggga agacgccgaa
     4381 gccaggcccc ccaggagtcg tcgggcaacg acacgcgcgt atagccctcg accgtattga
     4441 cgcgaagcag cagatcatcg acggcttcga ggggttcgag ggatgaatca gcgtgccaga
     4501 ggtagcgcgc aaggtccacg ggaccgatgg ttcgttccac ggctgacgtg gcagaggtcg
     4561 gagccgcggg agaaggggtt ggcaccgttc gaggtgattc ggatacctgc ggggtgggct
     4621 gcgccatcga tgcttctttg ggctcattct tcgtcgatgg gcaacctgct gcccacagag
     4681 cgaaaagggg caagaggcca attcgaaacg tatccgcctt catgatttct caggaatcat
     4741 ctgtttttta gggagcccgg ctgattcgaa ccgtagtccc gtttgggtat aggttagaac
     4801 atgacttgcc cctgcatcaa cacctcggtg gcccaatcca atttcgtttc cacgtccacg
     4861 gcgccgactt gcaatttcag gtttgcatgg tggcctttga tccagtagtt ggcaccgcca
     4921 aacaggccaa tgcgacgatt tccctcttcg gatggttggt agtattccac ctcgacgagg
     4981 ggctcgattt ttttgattcg gtatcccaaa tctccccaaa tccccacgcc tttcgggatt
     5041 gtgcctccag acccgtaatg gtgaccggcg acggtggcta cgatgccatt ttcgccgatg
     5101 ggcacatcga ccaaagcatc gaaagcgaga gcataaaagg tcttgttgcc cgtatcgccg
     5161 ccgacaccgg gttcgatatc gaaagaaaaa ccgaacgaga cgatgctctt ggagcccaag
     5221 tacgtgcctt tccagaaata gtctggctct acatccatca gattcacgcc caaacggcca
     5281 acgacgcggg gataatctcc ggcggcgttg ggtgccacgc catccgtgac ggcgagccgg
     5341 taatcgagaa cgtcatcgag gaccaaaccc cggaccaata ccccaagatc ccgatgcacg
     5401 ggtacgggcc cg
//
LOCUS       DAOSOR010000411         5386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15317_length_5386_cov_3.869255, whole genome shotgun sequence.
ACCESSION   DAOSOR010000411 DAOSOR010000000
VERSION     DAOSOR010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5386
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15317_length_5386_cov_3.869255"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="PK710_18435"
     CDS             <1..1203
                     /locus_tag="PK710_18435"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOT11761.1"
                     /translation="DAGVVTQDPTYFGQYVPVENEAALESFHRALGRLATARRDKKDT
                     KKVRMLAYGASHTQADVYTGYLRRYLQSRFGDGGQGFVLLGKVNSWHRMLDTKMENQS
                     LGYRHAHYKNDVENEPLGLFGAAFVGTTKNAYGEIIVSHDSPNTRFELHYYRQPKGGD
                     VEIMVDGQVVATVPTESETEEPAYYGFEATAGAHAIRVRLVGNGSVRLFGITAETDSP
                     GVVVDTLGIGGTRMPSALRWSESVWADAIRRRDPVLVTFAYGTNEAGNTAMAIAPYEQ
                     RVREVLARLRRAAPQTSCVLMAPFDFPLVHDDTLVRRPVLERIVQAQRRIAKDMGCGF
                     WDGYAFMGGAGSMKRWASANPPLASGDHIHLTRLGYTYVGIALADALMRRYDENPRYW
                     AELSAQSP"
     gene            complement(1488..3080)
                     /locus_tag="PK710_18440"
     CDS             complement(1488..3080)
                     /locus_tag="PK710_18440"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOT11762.1"
                     /translation="MPNEAKTPAESRSSPESPSGRNALIRVFWAGLGLFGAIAVPYLI
                     PTLYFLRPWVPGGEYVPFWNIVGRELLGEGKILQAEQEAMAAARLEVLTPALPVVHTE
                     PTEPAAERFPPYADNLRIEKPAFGIEPPEALDHFYEKLALADLGIPKAIARAGHWGDS
                     VLAIDGITSGIRRRLQTRFGDAGHGFHLMDRYDPGYRQRGIEYLRAGGWYSCFIVQQC
                     SKNEPFYGYGGLIARSGGGGMSTWRTPKEGFGSEVSFFELWFGRQTKGGKVEIVVDKD
                     PPIIVDTRGKDREDGWYAVTVPRGGHRFRVRAAGGGDVRLYGVVLENDGPGVVWDGMV
                     MIAGSTRQMRTQDATHISHQVRHRNLDLIVFLFGGNDMKRNHLDLKESMEPYYEEYGE
                     AIRKYRAGKPTMSCLVLSMTDRGERTVQGTVVSRKFAKQLSKAQREVAKRNGCGYFDT
                     YEATGGDGSIAKWYRANPPLISPDLGHPSAAGHEYIASLVANAILHGYQQYRDRMAGS
                     PLPTLEASDDPKKEAKDPLSGP"
     gene            complement(3162..4856)
                     /locus_tag="PK710_18445"
     CDS             complement(3162..4856)
                     /locus_tag="PK710_18445"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOT11763.1"
                     /translation="MLFASFDFLLFFAVTFSVYWALVGRPRARLLWIVGSSYFFYMAS
                     SKPVDGPLPTPWYFVGLLILSTIVDFFCGQRIAAARKRLPPDEPDGRAGKPWLLVAIL
                     TNLGLLGYFKYAGFLLEVARDICSVFGLAPHLPVVHALLPIGISFYTFQTLSYTIDVY
                     RGKIKAEPSFLRFAFFVAFFPQLVAGPIVRASEFLHQLHHRPKLTVDDVNEALWRISK
                     GVVKKVVFGDFIAAVFSDQVFASPATHSSLENLMALYAFTLQIYADFSGYSDIAIGVA
                     RLFHFRIPENFNRPYQAIDMADFWRRWHMTLSTWLRDYLYYPLGGSRVGETRTYINLG
                     ITMFLVGIWHGASWNFVIYASVQAGAMLFNRACRKLPPWSMARVLRIGSLTLLTTAAL
                     ALLGRLVLRIPDIWQFSLLGGAIALLIALLPRADTRRWTLPLHIGLTIHFSILSRIFF
                     RAEDLDAAREISLKLIQWDGLGVREGLFRIPNLSAWLEAIPSLSFARPLAEWGIFFLL
                     LVGFALHYTPAERVNRTAIQWISKTPAILLGVGLALIAGLLSILLAGPRANIYFAF"
BASE COUNT         1216 a         1412 c         1603 g         1155 t
ORIGIN      
        1 gacgctggtg ttgttaccca ggatccaacc tatttcgggc agtatgtccc ggtggaaaac
       61 gaagccgccc tggaatcctt tcatcgagcg ttgggtcgtc ttgcgacagc gcggcgggac
      121 aagaaagaca caaaaaaagt gcgaatgttg gcttatgggg cttcgcatac ccaggcggat
      181 gtttatacgg gataccttcg gagatacttg caaagtcgtt ttggagacgg gggccaagga
      241 ttcgtattgc ttgggaaagt gaattcttgg catcgaatgc tggacacgaa aatggagaat
      301 caatcccttg gttatcggca tgcccattat aaaaacgatg tcgaaaacga gccccttggg
      361 ctgttcggag ccgctttcgt cggaacgacg aaaaacgcct atggggaaat cattgtatcc
      421 catgattctc cgaatactcg ctttgaattg cattattatc gacagcccaa aggaggagat
      481 gtcgagatca tggtcgatgg gcaggtcgtg gcgaccgttc ccaccgaatc ggagacggag
      541 gaacccgctt attacggttt tgaggcaacg gcaggggcgc atgccatccg cgttcgtctt
      601 gtcggtaacg gttcggtacg actctttggt atcaccgcgg agacggattc gccaggggtg
      661 gtggtggaca cgttggggat aggggggacg cgaatgccca gcgcgctgcg gtggagcgaa
      721 tcggtttggg ccgacgccat ccgtcgacgg gaccctgtgc tggtgacgtt tgcctatggc
      781 accaacgaag ctggcaatac ggcgatggcc atagcgccct acgaacagcg ggtgcgtgag
      841 gtgctcgctc gcttgcgaag ggctgcgccg caaacgagtt gtgtgctgat ggcgcctttc
      901 gactttcctt tggttcacga cgataccctc gtccgtcgtc cggtgttgga aaggatcgtg
      961 caggcgcagc gcaggattgc caaggatatg ggatgcgggt tttgggacgg ttatgcattc
     1021 atgggaggcg cggggtcgat gaagcgttgg gcgtctgcca atccacctct ggcctcgggc
     1081 gaccatatcc acctgacaag gctgggatat acctatgtgg gaatcgcgct ggcagacgcg
     1141 ctgatgcgtc gttatgacga aaatcctcgg tattgggctg agctgtcggc gcaatccccc
     1201 tgatgatgtc ggatgtcgag gtacaccggg aggaagaagt cattgctcgg tccgcggcag
     1261 ggtcggcaag cccatcccaa gagtgagacc aggttatata aatcgtttta ttttccataa
     1321 cttacgagca ttcaatcgcc ttgtgtcgct tcactttcgc ggtggagcaa cctggatggg
     1381 atgggtaggc caagtcgtgg aaagatggcg gcggcggcgt gggccgatga gcttttcgtg
     1441 ctccgtttgg ccttcgcacg tgtcgccctt gcgacgaaga tggtccctta ggggcctgac
     1501 aatgggtctt ttgcctcctt ttttgggtcg tcggaagcct cgagggtggg cagcgggctt
     1561 cctgccatcc gatcgcgata ctgctggtag ccatggagga tggcgttggc gaccagcgag
     1621 gcgatatatt catgccccgc ggcgctggga tgcccgaggt cgggggaaat caagggggga
     1681 ttggcccggt accatttcgc gatcgagcca tcgccaccgg tggcttcata ggtatcgaaa
     1741 tagccacagc cattgcgttt ggccacttct cgctgggctt tggacaattg tttggcgaat
     1801 ttacgagaga cgacggtacc ttgtacggtg cgttccccgc gatcggtcat gctcagcacg
     1861 aggcaggaca tggtgggttt tcctgctcga tacttcctga tggcctctcc gtattcttca
     1921 taataaggtt ccatggactc tttgaggtcc aggtggttgc gtttcatatc gttgcccccg
     1981 aagagaaaga cgattaggtc caggttccga tgccggacct gatggctgat gtgggtggca
     2041 tcttgtgtcc gcatctgtcg ggtggagccg gcaatcatca ccattccatc ccagaccaca
     2101 ccggggccat cgttttcgag aacgacgcca tacaagcgaa cgtcgccacc gccggcggcg
     2161 cgtacgcgaa atcggtgtcc tccccgtggt acggtaacgg cgtaccagcc atcttcccgg
     2221 tcttttccac gggtgtccac gatgatcgga gggtccttgt cgaccacgat ttcgactttt
     2281 ccgcctttgg tttgtcgtcc gaaccacaat tcaaagaacg atacttcgct tccgaaccct
     2341 tctttcggtg ttctccaggt cgacattccg ccccctccgg aacgagcgat caaaccacca
     2401 tagccataga aaggttcgtt tttgctgcat tgttgtacga tgaaacagga ataccagcct
     2461 cccgcccgga gatactcaat cccccgttgt cgatagccgg gatcgtagcg atccatgaga
     2521 tgaaaaccat gccccgcatc accaaaacgc gtttgaagac gccgtcttat gccactggtg
     2581 atgccgtcga tggcgagtac cgagtccccc caatgccctg ctcttgcaat ggctttgggg
     2641 atcccaagat ccgcgagagc tagtttttca tagaaatggt cgagagcctc cggaggttcg
     2701 atgccgaaag cgggtttttc gatgcgaaga ttgtcggcat aggggggaaa acgttccgcc
     2761 gccggctccg tgggttcggt gtgtacgaca ggcagcgcgg gcgtaagtac ctctaaccgc
     2821 gcggcagcca tggcttcttg ctccgcttgc aggattttcc cctcgcccaa cagttctctg
     2881 cccacgatat tccaaaacgg aacgtattca cctccgggaa cccaaggacg caaaaaatac
     2941 aaggttggaa tcaggtacgg taccgcgatg gcgccgaaca accccaaccc agcccaaaaa
     3001 acgcgtatca acgcgtttcg cccggagggg gactccggag acgatcggga ttcggccggg
     3061 gttttcgctt cgttgggcat cgtagggact cgattggatt cacgggggat ggtaatgtca
     3121 agtcccaacg tcatcgggac ctgttgccgg gaccctactg ttcaaaaagc gaaataaata
     3181 ttcgctcgtg gaccggcaag aagaatactg agcagccccg ctatcaaggc tagccctacg
     3241 ccaagcagga tggccggggt tttcgaaatc cactgtatgg ctgttcgatt cacccgctca
     3301 gcaggggtat aatgcagggc gaaaccgacc agcagcaaga aaaaaattcc ccattccgcc
     3361 aggggacggg caaaggaaag cgaggggata gcttctaacc atgcggaaag attagggatt
     3421 ctgaataggc cttctcgtac gccaagtccg tcccattgga tcagcttgag ggatatttcg
     3481 cgggcggcat cgagatcctc cgcgcgaaaa aagattcggg ataggatgga aaaatggata
     3541 gtcaacccga tatgcaaggg aagggtccag cgtcgagtgt cggcacgggg cagaagcgca
     3601 atcaacaatg cgatggcgcc acccagcagc gagaactgcc aaatatcggg gatacggaga
     3661 acgagccggc ccagcaacgc gagggcagcc gtggttagca gggtcaacga cccgattcga
     3721 agcacacgcg ccatactcca cggtggaagc ttgcggcacg cgcgattgaa aagcatcgca
     3781 cccgcttgaa cactggcata tatcacgaaa ttccagcttg ctccgtgcca gatacccacg
     3841 agaaacatcg tgatgccaag attgatatag gtcctcgttt cccccacgcg gctgcctccg
     3901 aggggataat aaagataatc gcgcagccag gtcgataggg tcatgtgcca gcggcgccaa
     3961 aaatccgcca tatcgatggc ctggtacggt cgattgaaat tctcggggat gcgaaaatgg
     4021 aagaggcgcg ccacgccgat ggcgatatcc gagtagccgg aaaagtcggc atagatttga
     4081 agcgtaaacg cgtaaagcgc cataaggttt tccaaagagc tatgcgttgc cggagaggca
     4141 aacacctgat ccgagaatac ggcggcgatg aagtccccga aaacaacctt tttgaccacg
     4201 cctttgctga ttctccacag ggcctcgttg acatcatcga cggtgagctt gggacgatgg
     4261 tggagctgat gaagaaattc gctggcgcga acgatgggac ccgccaccag ttgcgggaaa
     4321 aatgcgacaa aaaatgcaaa acgaaggaaa gaaggctccg ccttgatctt tcctcggtac
     4381 acatcgatcg tgtagctgag ggtttgaaag gtgtaaaagc tgatcccaat gggaagcagg
     4441 gcatgaacga cgggcaggtg aggagccaat ccaaatacgg aacagatgtc ccgcgcgacc
     4501 tcgagcagaa accccgcgta cttgaagtat ccgagcaatc cgaggttcgt cagaatcgca
     4561 acgagcagcc atggcttccc ggctcgccca tcaggctcgt ccgggggcag gcgctttctc
     4621 gctgcggcga ttcgttgtcc gcaaaaaaaa tcaacaatgg tggataaaat cagaagccca
     4681 acgaaatacc atggtgttgg cagaggcccg tcgaccggtt tcgacgatgc catatagaaa
     4741 aagtagctgc taccgacaat ccagagcaag cgggctcgtg gtcgtccgac cagtgcccag
     4801 tagaccgaga aggtcaccgc aaaaaacagc aaaaaatcga aactggcgaa gagcattcag
     4861 ccatccatgc ggtcacgcgc cgtagcggaa tcttcctcct taccaagggg agttggctca
     4921 aaggcgcgtt gttttgtcag aaccacgggg cgagggactt tagcagagaa tccctggtgg
     4981 gggacaggat aggttcgact cgcttcggtt tggggttcct tatcagcttg gctgccagcg
     5041 ttggttccat tatggaatat tctttcgaag tttcatatag ttacgcattg tgcgctccgt
     5101 aatcattcgc acctaccctt gtcttcgatc tcgacgacac ggaatagcgc gccgcacggc
     5161 tgcgggtttt cgcgcggcta tgggtcgggc tgcggttctg gctgcggctc ggatggaatt
     5221 ggctatgcga acggcatagg cgttggttct tttccctcct atcatggggc tcacgacata
     5281 ggagtagcat gaacgactcc ctctccgtat ttccgaaaaa ttgcctccgg atggtggagt
     5341 tcgggtcgtg ctggacgcga cgccggctgt ggcgcctgat gctttt
//
LOCUS       DAOSOR010000412         5369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15375_length_5369_cov_4.263455, whole genome shotgun sequence.
ACCESSION   DAOSOR010000412 DAOSOR010000000
VERSION     DAOSOR010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15375_length_5369_cov_4.263455"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="PK710_18450"
     CDS             <1..1029
                     /locus_tag="PK710_18450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11764.1"
                     /translation="VPEARSSPHASREQAEVSPSRSVEHGQVSPMAHEPDWQARQRKR
                     GGTMPIPQPSAQPTPPPIAFEGPPPSPAAIDAVAAVPIVSIDAAAASATTPKDPAPEA
                     FVYGQPAYQSALSSAGSDSAVLSPTDTRKGLVWRTVVGGAWVTEALFLVPMATWYVAL
                     HLLLMHEHGAANLHESAWMTFGVATGALVTALLLWLVGLSCLSGRGWPRVYSVLAAVV
                     HGAWGITTITLFTRSYDSDSICWLIAVCVGSVLVSFALAVLTLSGHKWIGQGPVNGHA
                     RAIPYPLWAVPCCSSPPWCNCRASFGSSSFYSSNTTHIPGSRLPSAKCTEAYRFSSLG
                     WEDSSLPC"
     gene            1023..1355
                     /locus_tag="PK710_18455"
     CDS             1023..1355
                     /locus_tag="PK710_18455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11765.1"
                     /translation="MLIIVVSIALMRRSRWAVALGGGTGLLLLLVNVAAGIVLLATYG
                     KVVRYGFSFTLLDMGAASMWHFPVWLSLIAALRFRFRLPAYLRDAPAKDSNGKRLSLL
                     EILVPKQT"
     gene            1352..3178
                     /locus_tag="PK710_18460"
     CDS             1352..3178
                     /locus_tag="PK710_18460"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOT11766.1"
                     /translation="MNQRVFLGIVHALCTTIVLTSMGWTTLASAHDTGDLRWLDEFIP
                     EQLERHHIPGATIAWVEEGNVRWLRGYGVADRETQRAVDERTTFRIGSVTKLFVWTAL
                     WKLIEQGVIHLDDDVDEHLCGLSTPKRFSIPIRIRDLMNHTTGLESRRRGTYTDHEEE
                     LVPLEAWLGKEMRAQIRPPGRIESYSNFGTCLAARVVECREAMSFQQYFETRISTPLG
                     LRDSTLEQPLPPAKQATAARGYRGRGDAWVHHPFEFIRLAPAGAMSASARDMAAFMLA
                     LLGHAPHWMSATTLTSMLQTSHAPSSYLGGIAHGFFVGRRGSLRILWHGGDTLVFHSL
                     LMLVPERGLGVFVSYNAQGGAQAYRELMSAVIDRLDRTPSATSAHWIVPSSIEGAYRS
                     SRIAQSSYEKTEALMGDLEIRRSKNGVTAANQFWVPVAPWVFRQENGDDWLVAETGAD
                     GTVARVRRASYPMQTYERISLLEASKTHRSIAWFLFILLLTPLMWLPRGVRQTIGAGS
                     PRPSLLIGGINSIFGMFLLGLTEYMLRQGTDPWLRDPPWTVWAIRLCLSLLTITVATG
                     LWMVWLGWRRNWWSRWLRFHYAGMVIAGALLWTFAHHWGLSW"
     gene            complement(3271..>5369)
                     /locus_tag="PK710_18465"
     CDS             complement(3271..>5369)
                     /locus_tag="PK710_18465"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT11767.1"
                     /translation="FTMDDFSERPATPSRIGGLEQLGRFELICVLSRAEYGSSWACMT
                     TAPPNLGKVYSMRRIVVSPGDEDAVAKPILNAASVWEGVTRPGTLPIIDRVESSGRVG
                     IVSEYQEGEPLRSLIQLARLRNAPIPQPVVFRIATDLIQALVNLIGAALRKPELAANM
                     HGGLLPDAVLVTTSGETRLMEPGVTTEIGRIPSLARHEKIIAYQSPELLSDKKIDARS
                     DVFATGVVLWEMLTNKPLFLVVDGATDDKLGEAVAKRVLEEQAPRLDDASMGIAPAIS
                     KPLADVVERCLQKTINSRFSSLQDLATQFRKAVGAQVGTRKEVGAYLAELVGSSLETR
                     RSAIERASLNEPREEDSASATRSSTAPEVIPVSVDVDVDIGEGDDLEEQEDLLEEVSG
                     QFSPPVPPPRIAIKQPPSSRKPPPAANAPVTAPKQPSSPPSKPPSDLPPVPMPLDALA
                     SLAEDTKSDPASEPTQFDAARTEKALAQDAARQSPPEVSSNPFEQPFAPVAPEKPAPE
                     TAPAPEAQLAQSDKTPEPALSAASPEPETSVEAAVDSDKQEPPAAISPTAAFPSLPDT
                     DLEKNPDERKRKMMFIVGGVVAVAALVLVLGLIVSKSGKESTPSSANAALDSTPTTAV
                     PEPVPTPTETPEAAPPDVVEAAADTPKDAAVDAPSKTEKESETAASPKPATTKPAGGR
                     PGGKPKPKPTYTPSGI"
BASE COUNT          970 a         1473 c         1665 g         1261 t
ORIGIN      
        1 gttcccgagg cgcgttcatc accccacgcg agcagggaac aggctgaagt atcgccttca
       61 cggtcggtgg aacatgggca agtgagcccg atggcgcacg agcccgattg gcaagcacgg
      121 caacgaaagc ggggtggcac gatgcccatc cctcagccga gcgcccagcc gactccaccg
      181 ccgattgcct tcgaggggcc acctccttct ccagccgcga tcgatgccgt ggctgcggtt
      241 cccatcgtgt cgatcgacgc ggctgctgct agcgcaacga cccccaaaga cccggctccc
      301 gaagcttttg tgtacggaca acctgcctat caatccgcgt tgtcctccgc cggttccgac
      361 agtgccgttt tatctcccac ggacacacga aaggggttgg tatggcgaac ggtcgtcgga
      421 ggtgcatggg tcacggaggc gctgtttttg gtgccgatgg caacttggta tgtggccctt
      481 catctcttgt tgatgcacga gcatggcgcg gcaaacctgc atgagagcgc gtggatgacc
      541 ttcggtgtag ccacgggagc gcttgtgact gcactgctgc tatggctcgt cggcttgtct
      601 tgtctttcgg gtcgtgggtg gccacgcgtc tattctgtgc ttgccgcggt ggttcacggg
      661 gcatggggca tcaccacaat cacactattt acacgcagct atgacagcga ttccatctgt
      721 tggttgattg ctgtttgcgt tggctcggtg ttggtctcct tcgcgctcgc ggtcttgacg
      781 ctttccggac ataagtggat tggacaggga ccggtcaacg gccatgctcg cgccatcccc
      841 tatccattat gggctgtccc ttgttgctcg tctccgcctt ggtgcaattg tcgcgcgagc
      901 tttggaagct cgtcctttta ttcatcgaat acaacacata ttcccggttc tcgtctgccg
      961 tccgcgaagt gtacggaggc ctaccgcttt tcatcgttgg gatgggagga ctcgtcactg
     1021 ccatgctgat catcgttgtg tcgattgcgt tgatgcgccg ctcccggtgg gcggtggcgc
     1081 tgggcggtgg cacgggtttg ttgctgcttt tggtcaacgt ggctgctgga atcgttctgc
     1141 ttgccaccta tggcaaggtc gtcaggtacg gcttttcgtt cacacttctt gatatgggcg
     1201 ccgcctccat gtggcacttt cccgtttggt tatccctcat cgcggcgttg cgattcagat
     1261 tccgtttgcc ggcctatttg cgcgatgccc ctgccaagga ctcgaacgga aaacgccttt
     1321 ccctgttgga gatcctcgtt ccaaagcaaa catgaatcag cgcgtcttcc tggggattgt
     1381 ccacgcgctt tgcaccacga tcgtgctgac atcgatgggt tggactacct tggcgagcgc
     1441 ccacgataca ggggatcttc gctggctcga tgaattcatc ccggaacaac tcgagcgtca
     1501 tcatattccc ggtgcgacca tagcgtgggt ggaagaggga aacgtgcgat ggctgcgggg
     1561 gtatggcgta gccgatcgcg aaacacagcg tgcggtcgac gaacgaacga cgtttcgcat
     1621 cgggtcggtg accaaactat tcgtctggac ggccttatgg aaactcatcg aacagggagt
     1681 catccatctc gacgatgacg tcgatgagca tctttgcggt ctttccactc caaagcgttt
     1741 ttcgattccc attcgaatac gcgatctcat gaaccatacg acaggcttgg agtcgcgtcg
     1801 tcgtggcacc tataccgacc acgaggagga attggttccg ctcgaagcat ggttgggcaa
     1861 ggaaatgcgt gcgcagattc gtcctccggg gcgtatcgaa tcgtattcga acttcggcac
     1921 gtgcctggct gcccgcgtgg tggaatgcag ggaggcaatg agcttccaac aatacttcga
     1981 aacacgaatt tcgacaccgc tgggactgcg ggacagtacg ctcgagcaac cgttacctcc
     2041 cgcgaaacag gcgacggcgg ctcgcggcta ccgcgggcgc ggcgacgcat gggttcatca
     2101 tcctttcgaa ttcattcgtt tggctcccgc gggtgcgatg agcgcttcag cacgagacat
     2161 ggcggctttc atgctcgcgt tgctcggtca tgcgcctcat tggatgagcg cgacgaccct
     2221 gacttcgatg cttcagacga gccacgctcc aagttcgtac ctcggaggaa tcgcgcacgg
     2281 ttttttcgta gggcgtcgcg gctcgttacg gatcctatgg cacggcggag atacccttgt
     2341 ttttcatagc ttgctcatgc tggtgcccga acgcggcctg ggcgtattcg tttcgtataa
     2401 tgcgcagggc ggcgcgcaag cctatcgcga gttgatgagc gccgtcatcg atcgccttga
     2461 ccgcacaccg tcggctacgt cggctcattg gattgtcccc tcttcgatcg agggtgcgta
     2521 tcgttcgagc cggatagctc agtcgagcta tgagaagacg gaagcattga tgggggatct
     2581 ggagattcga cgatcaaaaa acggagtcac cgcggccaac caattttggg ttccggtggc
     2641 gccttgggtg tttcgtcaag agaacggtga tgattggctc gttgcggaaa ccggagcgga
     2701 tggaacggtc gcacgtgttc gaagggcgag ttatcccatg caaacctacg agcggatttc
     2761 gctcctcgaa gcctcgaaaa cccatcgttc gatcgcatgg ttcttgttta ttttgctgct
     2821 gacgcccctg atgtggctcc cacgcggtgt tcggcaaacc atcggcgctg gcagtccacg
     2881 gccaagcctg ctcatcggtg gaatcaatag tattttcggt atgttccttc ttggcctcac
     2941 ggaatatatg ctgcggcaag gcacggaccc atggcttcga gatcctccct ggaccgtgtg
     3001 ggcaattcgt ttgtgtttgt cgttgttgac gataaccgtc gccacgggac tttggatggt
     3061 ctggcttggt tggcgtcgga attggtggtc acggtggctg cgatttcact atgccgggat
     3121 ggtgatagcc ggtgctttgc tatggacctt tgcgcatcat tggggcttat cctggtagag
     3181 gatcgcaagc gcgtctcgcc tctaacagtt tgtaattatt agcatttatt gcaactagga
     3241 aaacctgaaa agggagtccg gcgagcggct tcaaataccc gaaggggtat aggtgggttt
     3301 tggttttggc ttgcccccgg gtcttccgcc ggccggtttg gtggtggcgg gtttcggact
     3361 ggccgcggtt tcgctttcct tttctgtttt cgaaggagca tcgacggcgg cgtctttggg
     3421 cgtatccgcg gcggcttcaa ccacgtcggg gggcgcggct tcaggagtct cggtgggtgt
     3481 tggcacgggc tcgggcaccg cggtggttgg ggtcgaatcc agcgctgcat tcgcactcga
     3541 aggcgtggat tccttgcctg atttcgagac gatgagacca aggaccagca ccagcgcggc
     3601 aacggccacg acgcccccca cgatgaacat catctttcgt tttcgctcat cgggattttt
     3661 ttcgaggtcg gtgtcgggca aggaaggaaa agctgcggtt ggcgagatgg ccgcaggcgg
     3721 ttcttgcttg tcggaatcga cagcggcctc cacggacgtt tcgggttccg gggaagctgc
     3781 ggaaagcgcg ggttccgggg tcttatcgga ttgcgcaagc tgggcttcgg gcgccggcgc
     3841 ggtttcgggc gctggttttt ccggagcaac gggcgcgaag ggctgttcga aaggattgga
     3901 agaaacttcg ggcgggcttt gtctcgcggc gtcctgcgcc aaagcttttt ccgtgcgtgc
     3961 ggcgtcgaat tgggtaggtt ccgacgcggg gtccgacttt gtatcttccg ccaacgatgc
     4021 gagtgcatcc aggggcatgg gaaccggagg gagatcggaa ggtggtttcg aaggtgggga
     4081 agacggttgt tttggcgcgg tcacgggcgc attcgcggct ggtggcggtt ttcgactgct
     4141 ggggggctgt ttgatagcga tccgcggcgg gggcaccgga ggagaaaact gtcctgatac
     4201 ttcttcgagc aaatcctctt gttcttccag gtcatcgcct tcgccaatgt ccacatcgac
     4261 atcgacactc acggggatga cttcgggtgc cgtagacgaa cgggtcgcgc tggcggaatc
     4321 ctcttcccga ggctcattca acgacgcgcg ttcgatcgcg gagcgtcggg tttccaagct
     4381 cgatcccacg agttccgcca gatatgcccc tacttctttg cgggtgccca cttgtgctcc
     4441 cacggctttt cgaaactgcg tcgccaagtc ctggagcgaa gaaaaccggc tattgatggt
     4501 tttttgaagg caccgttcca ccacgtccgc cagcggcttg ctgatggctg gagcaatccc
     4561 catcgatgca tcgtcgagcc gtggagcttg ctcttcgagc acgcgcttcg cgacggcctc
     4621 gccaagtttg tcgtccgtag ccccatcgac gaccaaaaac agcggtttgt tggtcaacat
     4681 ttcccacagc acgacacccg tggcaaagac atccgatcgg gcgtcgattt tcttatccga
     4741 aagcaattct gggctttggt aggcaatgat tttttcgtga cgtgcgagcg aaggaattct
     4801 tccgatctcc gtggtgacac ctggttccat gaggcgagtc tcacccgagg tggtgacgag
     4861 aacggcgtcg ggaagcaagc caccgtgcat gtttgcggcc aattccggtt tgcgcagagc
     4921 cgcgccgatc aagttgacga gagcttgaat cagatcggtg gcgatacgaa acacgacagg
     4981 ttgggggatg ggggcattgc gcaagcgcgc gagttgaatc aaagagcgca acggttcgcc
     5041 ttcttggtat tcggagacga taccgacgcg gcccgaggac tcgaccctgt cgatgatcgg
     5101 aagggttcct ggccgtgtga cgccttccca caccgacgcg gcgttgagaa tcggttttgc
     5161 gacggcatcc tcgtcgccgg ggctgacgac gatccgtcgc atggaataga cttttccgag
     5221 attcggcggt gcggtagtca tacaagccca ggaagaaccg tattccgcgc gcgaaagaac
     5281 gcagatcaat tcaaaccggc caagctgctc caaaccaccg attcgagagg gggttgcagg
     5341 ccgttccgag aaatcatcca tggtaaatt
//
LOCUS       DAOSOR010000413         5368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15378_length_5368_cov_3.997553, whole genome shotgun sequence.
ACCESSION   DAOSOR010000413 DAOSOR010000000
VERSION     DAOSOR010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15378_length_5368_cov_3.997553"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..153
                     /locus_tag="PK710_18470"
     CDS             7..153
                     /locus_tag="PK710_18470"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11768.1"
                     /translation="MDERFVRSLEEGMPSAGGNALGVDRLVALCLGARSIGDVMAFPD
                     SWLW"
     gene            209..1021
                     /locus_tag="PK710_18475"
     CDS             209..1021
                     /locus_tag="PK710_18475"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT11769.1"
                     /translation="MPHEWPLDPHSFSKYYAFAPTALALRECIRLQAVRHLELPEPIL
                     DVGCGDGVFARLAYPGKQTWGIDINPTELQRAQGTHSYSTLICGNICTVDLPPQFFGS
                     AIANCSLEHVPDLRAALRNVRSALKPGAPLVLIVPTPTWTRRLALTEALGKAGLHSLG
                     NAWGELLDHVFRHVHRIDAATWMRHLEACGFEACEVTMIAQRGVSWAFDILLWPSLVG
                     FLNKKLTGRWVLLPRLRFLTSDLMRISLNALASRVPDAEEAAEYLIVCKARP"
     gene            1018..1668
                     /locus_tag="PK710_18480"
     CDS             1018..1668
                     /locus_tag="PK710_18480"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT11770.1"
                     /translation="MMSESEFSAEYYTNHERRDRFPWTLYHRPISRRIASIVRARSTL
                     PTILIVGCGLEPYVDGVREISCAACDIDPRAIEACKKQYPRQADRFGVSPSAYELPSA
                     EVFDGPFDMIVAKDVVEHVRQPERWARMLAQRLSPGGELLLSTPNYGTGSTLGLLERT
                     VLEWVARRDGYSRRHIHPSRFDAGLFAGLNVGDGMELMEIWHTWNRWAMVGRWRRK"
     gene            1726..2553
                     /gene="larE"
                     /locus_tag="PK710_18485"
     CDS             1726..2553
                     /gene="larE"
                     /locus_tag="PK710_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOT11771.1"
                     /translation="MIDKKLDALRERLRQLESVVVCYSGGLDSGFTLAVAHEQLGHHA
                     VGLTAAGPALAPRERDDAIAFARQIGADHEMVDAGEIGVADYVANGPDRCFHCKSALY
                     VAAERYRQQRGFRHVINGTNLDDLGDYRPGLEAAKRANIVSPLVDVGFTKSEVREAAR
                     LLGLRLWDKPAAACLASRIPYGTAVTSKRLSQIAGFEAELRDLGFRVVRVRHHDTIAR
                     IEVGEDELAAMADPTTRQRVHDAGKRYGFSYVTLDLGAYRVGSHNEVLDGRRLRTVT"
     gene            2629..>4084
                     /locus_tag="PK710_18490"
     CDS             2629..>4084
                     /locus_tag="PK710_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT11772.1"
                     /translation="MNDYRHGRGELQMVFVPKFLTIVDLFEKSVEKYAPRDLFGVKTN
                     DAWTWITYGEVKRRVDAFRRALSEEGIARDGRVAIISNNRVEWAIASFATVGLTAQFV
                     PMYESQLPKEWEYILRDCEAEILIVSTEAIYHKVKGFLETIPSLRKIVRMGAKESEPD
                     SFDAWIDRGSDNPLEPSVPDKDDIAFLIYTSGTTGNPKGVKLSHENIASNVSAVHECF
                     DMIADDRTLSFLPWAHSFGLTCELHCMTSMGASMALNTAVDKLVAEMPEVRPTILISV
                     PRIFNRIHDNVQKQIAGKPAPIQALFRAGLAASIKKRAGQSLGLTERVSLSLADKIVF
                     SKIRAKFGGQLRYAISGGAALSREVGQFIDALGINVYEGYGLTETSPIISANRPGHRV
                     IGSVGQMIPGCRVVIDKEATADAVQGEIIAYGPNVMKGYHHLDEETAAVLLEDGGFRT
                     GDMGYMDNNGYLFITGRVKEQYKLENGKYVVPVPL"
     assembly_gap    4085..4094
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4095..4437
                     /locus_tag="PK710_18495"
     CDS             <4095..4437
                     /locus_tag="PK710_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOT11773.1"
                     /translation="SLLITNAFVHGANRPFNVALIVPNMEELVEWAKAQGIEDVSAKS
                     LLRNEKVIAKVREEIEAKSQGFKSYEKIKQFTLIEEDFTTENGMLTPSMKVKRRVVLE
                     KYGNLVEDMYR"
     gene            complement(4441..4782)
                     /locus_tag="PK710_18500"
     CDS             complement(4441..4782)
                     /locus_tag="PK710_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11774.1"
                     /translation="MSKLFPFTVLAALGAGMIAGCNTPTLPLPPPSEGAIVAQLDLPS
                     GEARVIGKSQAVEAYALVTCFNNRTGGGVVGLANGNGGFEIRVAAQQGDVIEVWQRVH
                     NDPPSVVVSVE"
     gene            5145..>5368
                     /locus_tag="PK710_18505"
     CDS             5145..>5368
                     /locus_tag="PK710_18505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11775.1"
                     /translation="MRDIRSALRHFPWKPVPWALGLAIVVTGATCVPVDRDGDPRGAL
                     VAALSRDGYVASVEDVHFFGPPSTLGTTRGV"
BASE COUNT         1107 a         1332 c         1702 g         1217 t
ORIGIN      
        1 tatccaatcg atgagcgatt cgttcgaagt ctggaggaag ggatgccgtc ggcggggggc
       61 aatgcactcg gggtcgatcg attggtggcg ctttgtcttg gagcccggtc tattggtgat
      121 gtgatggcgt ttccggactc gtggttgtgg tagctggcga tggttccgga aacgatattt
      181 tctatgtatt tacgataggt tagaaccgat gccccacgaa tggcctctcg atcctcactc
      241 cttttccaag tactacgctt tcgcgcccac ggcattggcg ttgcgtgaat gcattcggct
      301 tcaagccgta aggcacctcg agttaccgga accgattttg gatgtgggat gcggagatgg
      361 tgtgtttgcg cgtcttgcct atccaggcaa gcagacgtgg ggcatcgaca tcaacccgac
      421 ggagttgcag cgagcgcagg gaacgcattc gtacagcacg ttgatttgcg gcaacatctg
      481 cacggtcgat cttcctccgc aatttttcgg cagcgcgatt gccaattgca gtctcgagca
      541 tgttcccgat ctgcgagccg cgttgcggaa tgttcgtagc gcattgaaac cgggagcccc
      601 cctggtcctc atcgtgccta cgccgacgtg gacacgtcgc ctggcattga cggaagcgct
      661 tggcaaggcg ggattgcata gtctcggaaa tgcttggggg gagctactcg atcacgtgtt
      721 tcgtcatgtt catcggatag acgcggcgac atggatgcgg catttggaag cctgcggatt
      781 cgaagcatgt gaggtgacga tgatcgcgca acgcggtgta tcgtgggcct tcgacatcct
      841 actttggccg agccttgtgg ggttcctcaa caaaaaattg accggacgat gggtgctatt
      901 gccccgtctg cgtttcctca ccagcgatct catgcgcatt tccttgaacg cccttgcctc
      961 gcgcgtgcct gatgccgaag aggctgcgga gtacctcatc gtttgcaaag cgcggccatg
     1021 atgagcgagt cggagttcag cgccgagtac tatacgaacc acgagcgacg cgatcgcttt
     1081 ccttggaccc tgtaccatcg tccgatttct cgacgaattg catcgatcgt gcgtgcgcgg
     1141 agcacgttgc cgacgatcct gatcgtgggg tgtggcctcg agccgtatgt ggatggggtg
     1201 cgggagatat cgtgtgcagc gtgcgacatc gatccgcgtg cgattgaagc gtgcaaaaaa
     1261 cagtatccac gacaagcgga tcgctttggg gtcagtcctt ccgcctatga attgccgtcg
     1321 gccgaggttt tcgacggccc cttcgacatg attgtggcaa aagacgtggt cgagcacgta
     1381 cggcaaccgg agcgttgggc gcggatgctt gcgcaacgtc tttcgccggg tggggaacta
     1441 ttgctttcca ctccgaatta cggaactggt tcgacgttgg ggttattgga gcgaacggtt
     1501 ctcgagtggg tagcccgtcg ggatggttat tcacgtcgtc atattcatcc aagccgcttt
     1561 gacgcggggc tatttgcggg attgaacgtg ggcgacggga tggaattgat ggagatatgg
     1621 catacgtgga accgatgggc gatggtgggc cggtggcgtc gcaaatagag aaaaacgaag
     1681 ttgccaccga tgacgatgcc gggccggggc tttatgagga catctatgat cgataaaaag
     1741 ttggatgcct tgcgtgagcg gttgcggcaa ctcgagtccg tggtggtttg ttattccggg
     1801 gggcttgata gtggatttac gctggcggtt gcgcacgaac agcttgggca tcatgcggtc
     1861 ggattgacgg cggcggggcc agcgttggcg cctcgtgaga gagacgacgc gattgcgttt
     1921 gccaggcaga ttggcgcgga tcatgagatg gtggatgctg gggagattgg tgtggcggac
     1981 tatgtggcca atggcccaga ccgttgtttt cactgcaaat cggcgttgta cgttgcggcg
     2041 gagcgctatc gtcaacaacg aggttttcgt catgtgatca acggtacgaa cctcgatgat
     2101 ttgggggatt accgtcctgg gctcgaggcg gcgaaacggg cgaatatcgt cagccctttg
     2161 gttgatgtgg gatttaccaa gagcgaggtg cgagaggcgg ccaggctgtt ggggttacga
     2221 ctttgggaca agcctgcggc ggcctgtctc gcgagtcgga taccgtacgg gacggcggtc
     2281 acctccaagc gtttgtcgca aatagctgga tttgaagcgg aacttcgtga tctggggttt
     2341 cgtgtggtcc gggttcgtca tcacgacacc attgcccgta tcgaggtggg agaagacgaa
     2401 ttagcggcga tggcagaccc aacgacgcgg cagcgagtgc atgatgctgg aaaacgatat
     2461 gggttttcat atgtaacgtt ggatctggga gcctaccggg taggtagtca taacgaggtt
     2521 ctcgacgggc ggcggctccg aacggtgacg tgaagtaccg tcacggattg acccggtgtc
     2581 gttctgacgc ttacgctagg cataaacgcg gattggttca gccaaaccgt gaatgattac
     2641 cgacatggac gaggggaatt gcagatggtg ttcgttccta agtttctgac cattgtggat
     2701 ctattcgaga agagcgtcga aaaatacgcc ccgcgagatc tgttcggcgt gaagaccaac
     2761 gatgcctgga cgtggattac ctacggtgag gtgaaacgtc gcgtggatgc atttcgacga
     2821 gcgttgagtg aagagggaat cgcgcgcgat ggccgtgtag cgatcatctc gaacaaccgc
     2881 gtcgaatggg ccattgcttc gtttgcgacg gtcgggctga cggcacaatt cgttccgatg
     2941 tacgagtctc aattgcccaa ggagtgggag tacattcttc gcgattgcga agcggagata
     3001 ctcatcgtat cgacggaggc gatttaccat aaggtcaagg gatttctcga aacgatacca
     3061 tcgctcagaa agatcgttcg catgggggcg aaggagtctg aaccggattc ttttgatgct
     3121 tggatcgaca ggggatctga caaccctctt gaaccatcgg tccctgacaa agacgatatc
     3181 gcatttttga tttatacgtc gggaaccacg ggcaacccga agggggtcaa gttatcgcac
     3241 gaaaatatcg catccaatgt atccgcggtg catgagtgtt tcgatatgat tgcggatgac
     3301 aggaccttgt cattccttcc ctgggcgcat tccttcgggc tcacttgcga acttcattgt
     3361 atgacgtcga tgggagcgag catggcactc aacacggcgg tggataaact cgtggcggag
     3421 atgccggagg ttcgtccgac cattttgatt tcggtgcccc gcatttttaa ccggatccat
     3481 gacaacgtgc agaagcagat cgcgggaaag ccagcgccga ttcaagcact atttcgggcg
     3541 ggacttgcgg catcgattaa gaagcgcgcg ggtcagtcgc ttggtttgac ggagcgagtg
     3601 tcattgtcgc tggcagacaa gatcgtattt tccaaaatcc gtgcgaagtt cggtggtcag
     3661 ttgcgctacg cgatttccgg tggcgcggct ctctcgcggg aggtggggca gttcatcgac
     3721 gccttgggca tcaacgtgta cgaaggctat gggttgacgg aaacgagtcc catcatttcg
     3781 gcgaatcgac cggggcatcg tgtcatcggc agtgttggcc aaatgattcc cggatgccgg
     3841 gtggtcatcg acaaagaggc cacggcggat gcggtgcaag gagaaatcat tgcgtacggt
     3901 cccaacgtca tgaaggggta ccaccatctc gacgaagaga cggccgccgt gttgttggag
     3961 gatggcggct ttcgcacggg cgacatgggg tatatggaca acaacggcta tttgttcatc
     4021 acggggcggg tcaaggagca gtacaagctc gagaacggca agtacgtggt tcctgttccg
     4081 ctcgnnnnnn nnnngtccct gctgatcacc aacgcgttcg tgcatggcgc aaacagacca
     4141 ttcaacgtgg cgctgatcgt tcccaacatg gaagaattgg tcgagtgggc gaaggcgcag
     4201 ggcatcgagg atgtttcggc gaagtcgttg cttcgcaatg aaaaagtcat cgcgaaggtg
     4261 cgtgaagaga tcgaagctaa gagccagggc ttcaaatcct acgagaaaat aaagcaattt
     4321 acgctcatcg aagaggactt caccaccgaa aacgggatgc tgacgccatc gatgaaggtc
     4381 aagcgccggg tggtgttgga gaagtacggt aacctggtgg aggatatgta ccgctgacgc
     4441 ttactcgacg gaaacgacca cactaggcgg gtcattatgg actcgctgcc acacttcgat
     4501 gacatcccct tgttgggccg caacgcgaat ctcgaaacca ccattaccgt tggccagtcc
     4561 taccacgccg cctcctgttc gattgttgaa acacgtcacg agagcatacg cttccacggc
     4621 ttgggatttg ccaatcaccc gggcttctcc gctgggcaag tccagttgcg cgacgatcgc
     4681 gccttcggac ggaggcggca acggaagggt tggtgtgtta cagcctgcga tcattcccgc
     4741 gccgagggct gcgagaaccg tgaatgggaa gagcttcgac atattcatcc aatcctattc
     4801 atcgaacgca tgaccttcga cggaaaaggg caaagcgcgc tcgaatcgag agccgttcgt
     4861 tcgatgaatt catccggctc atcaaaggat gatggccgcc tcgagcaacg gcaaggcctc
     4921 catatcattc gagcctggct tctcccatcc gaaggagtgt ggatggtggg aacttagcga
     4981 tgaggcacgt ttctgccctt gcccaagggg ccctgtctgg acgtgcgctc ccagtatctc
     5041 gaatgcgata ccgtggtgcg gtcggccaat ggcgcaaaat cgcaaccagg tggcatctcc
     5101 caccggaagg tgctaggcgc tggaagcgtt ttcatctcga tgccatgcgc gacatccgct
     5161 ctgctctccg acactttccg tggaagcccg ttccttgggc gctgggtctg gcgatcgttg
     5221 tcaccggggc aacgtgtgtt cctgtcgacc gtgatgggga tccgcgaggt gcgttggtgg
     5281 cggctctttc gcgtgatggc tatgtggcgt ccgtcgagga cgtccatttt ttcgggcccc
     5341 catcgacctt gggcacgacg cggggggt
//
LOCUS       DAOSOR010000414         5367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15379_length_5367_cov_4.191642, whole genome shotgun sequence.
ACCESSION   DAOSOR010000414 DAOSOR010000000
VERSION     DAOSOR010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15379_length_5367_cov_4.191642"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PK710_18510"
     CDS             complement(<1..840)
                     /locus_tag="PK710_18510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11776.1"
                     /translation="MRMILAWALLILMVGVAGGVRAEPGVAAPSTASAPSSSSTLDLA
                     MGSASVGPPVSGSPPGERTAEPSPSSVSFPVATASAAASASASAASSSRAVVTTAIPR
                     DYGLNLGLGAAPPTVVRDTPRASFQGFLDASRRNDYETAAHYLYLWGIPPGRQKAEGP
                     ELAQRLAEAMDRAVWVDIDQIPDTPTGSNVEGASPLDDVRIATVETDRGSHTLRLART
                     RDPFSSGMVWVFSASTVRGIDSLDATYGLPDVVHKLPAWLSQDKAFGLLWFQWIGLVL
                     LAVG"
     gene            complement(1087..4203)
                     /locus_tag="PK710_18515"
     CDS             complement(1087..4203)
                     /locus_tag="PK710_18515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT11777.1"
                     /translation="MTPVWLGIALPWICLVLGLLALVAFARRAFQKVQLHRRPVLLAG
                     LAVAALLALYVVLVWAGVVPDRTLRVERPFGVALTLLAAGFVAWRLAGLSRRQSNLRR
                     SLVEGFGMLATLLAGMSIAGMDLGRPLDKLTVLVAVDRSRSIDLVPGVEERIRMDLRV
                     AESGMRERDRIGTIVFGAQALVEDPPHPRSDLPMPQRVEVGRDGTDLGAAIRRSLAEV
                     PSDSAARIVVISDGVSNRGDPEAAAAAAVAAGIPIDTIALDQREVKDIRVVAVRMPSR
                     ASEGETLDMHLVTASSEPADVEVRIKRDGQLIAKSVTKIEAGEDVLRIREKAPGAGLH
                     RFDVEVSAVNPALDQAPEDNAGSAFVRVRGPATALVLEGEEGKGAFIARALQNVNYAV
                     QTGGASAVPSDIGGMAPYDVIFFSDIPAFMLSPSQIEALATYVRDLGGGLVLMGGDRS
                     MGPGGYSQTPLEEVSPVSFDIKQDRRRASLAEVIIIDYSGSMAAHAGKNTKLELANEA
                     SVRSANLLGPGDRLGVMHVDTVVDWTIPLGNVTNPEEIAKKIRAVGPGGGGIYVDISL
                     RDAYAALDRETVNLKHVLLFADGGDAEQLAGCRAQVSNAKRRGITTSVVALGRGSDVP
                     ELEVLSKLGDGRFYLIEDATRLPTIFTQETILAARSALNEVDFRVTSGASSPPTRHID
                     FSESPILKGYVVTIPKARSTVMLTGPEGDPVLASWSVGMGRAAAFTSDLKDRWGRHWT
                     TWAGASSLVAQLARDVTRLADDPKVRLDADASGGQLHIRAQVVDDKGRAQTFRRLSAF
                     IAGPDGISFAMPLEPVGAGVYAATQPLSRPGTYVVTAKDELDNKIVSTTGAVLTAGEE
                     MRPTGTDRLLLARISSMTGGKTRDTLAGVFADRSAFRFSYWPLTPWLSGLGAVFLLLS
                     VGARRLSVPTAWLQRRRKPSTQKAEKAQKTVQEVTATLDRLGKAKERAWAGESKGAGQ
                     GHAVARPAPVAWQPPPMGSSVIAQQRPTVSATVQPAEPGARMSSPPSARPLTAAEILL
                     QKRKGRRG"
     gene            complement(4200..>5367)
                     /locus_tag="PK710_18520"
     CDS             complement(4200..>5367)
                     /locus_tag="PK710_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT11778.1"
                     /translation="FVTLRQHNASDVLASRKVLLQPGEKAPVVLSFEAVPGDIGQGLL
                     VQLSPADAMPVDDVAYARVPPGEEISVIGLGKRSPWIERAFRSDPNVAWEEGSVSDLE
                     SGAIPPGALVVIEGQCPTVLPPGDMLILNPPEGPCLTTTVKGLVDKPMITSWATADQR
                     FRFLTLDGVLMEKARLLGVDNPRHELIHAREGAIAADVSLPGRTVTLVGFDVGDTNWP
                     YKASFVLFVRNLVELARTHRSHGVVGAGKAGEPVRLAVPHHVQEVKVVGPGEVTQSLR
                     ARDGLAIVPSTQKAGINHASWGKPIPGSVVFAINLTSENESDVRDKPLEFTSSDVKTT
                     TAEQVSQSHTEWSWLLAVLALAAVITDVWYLTRKPRFRSLSATLQPKRPERTAT"
BASE COUNT         1165 a         1896 c         1338 g          968 t
ORIGIN      
        1 ccccactgcc aatagcacca acccaatcca ctgaaaccac aaaagcccga acgccttgtc
       61 ctggctcaac cacgcgggaa gcttgtgtac cacgtcagga agcccgtacg tcgcatcgag
      121 actgtcaatg ccgcgcaccg tgctcgcgga aaatacccag accatgccgc tcgaaaacgg
      181 atcccgcgtt cgcgccaatc gcaacgtatg cgaacctcga tccgtttcca ccgtcgcaat
      241 ccgaacatcg tcgagcggcg atgccccctc cacattcgaa cccgtcggcg tatccggtat
      301 ctgatcgata tcgacccaga ccgctctatc catcgcttcc gccaaccgct gcgcaagctc
      361 cggcccctcg gctttttgac gccccggcgg aatcccccac aaatataagt aatgcgccgc
      421 cgtctcgtaa tcgtttcgcc gggaggcgtc gagaaagcct tgaaaagacg ctcgcggcgt
      481 gtcacgcacc accgtcggtg gcgccgcccc caacccgaga ttcagcccgt aatcccgcgg
      541 tattgccgtt gtcaccaccg ctcgcgacga actcgccgcc gatgccgacg cagatgccgc
      601 tgcagatgcc gtcgccacgg gaaaggaaac cgacgacggc gatggctcgg ccgttcgctc
      661 gccagggggc gaacccgaaa ccggaggccc caccgaagca gaccccatcg ccaaatcaag
      721 ggtcgatgac gaagaagggg cggatgcagt cgacggagca gctacccccg gctccgctcg
      781 tacgcctcca gccactccca ccatgagaat caacaaggcc caagccagaa tcattcgcat
      841 cacgacctcg ctctagcacg accatgcctt tggcgaacac gcttgttatc gggacatcaa
      901 cccccggaat tcctttccac ggtgactcgc cgattgctgt ttcccttccc ccacaccgag
      961 cctcccgcgg aatgtacgcc ccgccaagaa aacgaacgtt tgtgtccgta ttagatatta
     1021 tctcaataaa atcaatatat tacatcgatt tctctcgcca agacccagtc ggcgctctgc
     1081 ctgccatcac cctcgtcggc ccttgcgctt ttgcaacaaa atctccgccg ccgtcagcgg
     1141 tcgcgcactc ggcggcgacg acatcctcgc gcctggttcc gccggttgca ccgtcgcgct
     1201 cactgtcggt cgctgttgcg cgataacgga cgagcccatc ggtggcggtt gccatgccac
     1261 cggtgctgga cgagccaccg cgtgcccttg tcccgcccct ttgctttccc ctgcccacgc
     1321 ccgttctttg gcctttccca gcctatccaa cgttgccgta acctcctgca cggttttttg
     1381 cgccttttcg gccttctgtg tcgagggctt tctccgacgt tgcagccacg ccgtgggaac
     1441 ggatagccgt cgtgccccga ccgacaacag caaaaacacg gccccaagcc ccgataacca
     1501 gggcgtcaac ggccaatagg aaaaacgaaa cgccgatcga tccgcaaaaa cccccgcaag
     1561 cgtatcccgc gtctttcccc ccgtcatcga agagatccgc gccaacaaca agcgatccgt
     1621 tcccgtcggg cgcatttcct ccccagccgt caacaccgct cccgtcgtgc tcacaatttt
     1681 attatccaac tcatctttcg ccgtcaccac ataggttccc ggacggctga gcggttgcgt
     1741 ggcggcgtaa accccggcac ccaccggctc caacggcatg gcgaaagaaa tcccatccgg
     1801 ccccgcaatg aaggccgata accggcgaaa cgtttgcgcc cgccccttgt cgtcaaccac
     1861 ctgcgcccga atatgcaatt gcccccccga cgcatccgca tccagacgca ccttgggatc
     1921 gtcggccaat cgagtcacat cccgcgcaag ttgggccacc aacgacgaag cgccagccca
     1981 ggtcgtccaa tgacgtcccc agcggtcttt taaatcgctc gtaaacgcgg ccgcacgccc
     2041 catccccacc gaccaactcg caagcacggg gtctccttcg ggacccgtca acatcaccgt
     2101 ggaacgggct ttgggtatcg tcaccacata ccctttgaga atcggtgact cggaaaagtc
     2161 gatatggcgt gtaggaggcg aacttgcccc tgacgtcacg cgaaaatcca cttcgttcag
     2221 cgccgatcga gcggccaaaa tcgtctcctg cgtaaaaatc gtcggaagcc tcgtcgcatc
     2281 ctcgatcaaa taaaatctcc cgtccccaag cttcgatagc acctccagct ccggcacgtc
     2341 gcttcccctg ccaagcgcta ccacgctggt cgtaatgccc cttcgcttgg cattcgatac
     2401 ctgcgcccga caccccgcca gttgctcggc gtcgccaccg tccgcaaata acagcacatg
     2461 cttgagattg acggtttccc ggtcgagggc cgcataagcg tcacgcagtg aaatatcaac
     2521 gtaaattcca ccacctccgg gaccaacggc gcggattttt ttcgctatct cctccggatt
     2581 cgtcacgttt ccaagaggaa tcgtccagtc caccaccgta tccacatgca tcacgccaag
     2641 acgatccccc ggacccaata ggttcgccga ccgcaccgaa gcctcgttgg ccaattcaag
     2701 cttggtattt ttccccgcat gcgcggccat cgaaccggaa taatcgatga tgatcacttc
     2761 cgccaaactc gctcgacgac gatcctgttt gatgtcgaaa gatacgggag acacctcttc
     2821 caacggggtt tgggaatatc ctccaggacc catcgaacga tcgcccccca tcaacaccag
     2881 cccaccaccc aggtcccgca cataggtggc cagcgcttcg atttgcgacg gtgacagcat
     2941 aaaagcaggg atatcgctga aaaaaatgac atcgtaagga gccatgcccc cgatatccga
     3001 aggtaccgcg ctcgctccgc ccgtctggac cgcataattc acattctgaa gagcccgggc
     3061 aatgaacgcc cctttgccct cttccccctc gagcaccaag gccgtcgccg gtcctcgcac
     3121 gcgaacgaat gcactgcccg cattgtcctc cggcgcctga tccaacgcgg gatttaccgc
     3181 cgaaacctcc acgtcgaaac ggtgaagccc ggctccaggc gctttttctc gaatgcgaag
     3241 cacgtcttcc cccgcttcga tcttcgtcac gcttttggcg atgagctgcc catctcgctt
     3301 gatccgtacc tccacatccg cgggctccga tgacgccgtg actagatgca tgtcgagcgt
     3361 ttccccttca ctggcacgac tgggcatccg taccgcaacc acccgaatat ccttcacttc
     3421 acgctggtcg agggcaatcg tatcgatggg aatccccgcc gccaccgccg ctgccgctgc
     3481 cgcttccgga tctcctcgat tcgatacacc atcggaaatg accacgatac gcgccgccga
     3541 atcggacggc acttccgcca aagagcgacg gatcgcggca cccaaatccg ttccatctcg
     3601 acccacttcc acacgctggg gcatgggcaa atcactacgc ggatgggggg gatcctccac
     3661 gagcgcctgg gcaccaaata caatcgtgcc gatgcgatcc cgctccctca ttccactttc
     3721 ggcaacgcga agatccatgc ggattcgctc ctccaccccc ggtaccaaat cgatggaacg
     3781 gctccgatcg accgccacca gcaccgtcag cttgtcgagc ggtcgcccca gatccattcc
     3841 cgcaatcgac atccccgcaa ggagcgttgc caacatgccg aaaccttcca cgagcgaacg
     3901 tcgcagattg gattgacgac gggacaatcc cgcgagacgc cacgccacaa atccggccgc
     3961 cagcagcgtg agcgccacgc cgaaaggtcg ttccacgcgc aaggtgcgat caggtacgac
     4021 ccccgcccag accaagacga cgtaaagggc caatagagcc gccacggcaa gtcccgcgag
     4081 cagcacgggg cgacgatgaa gctgcacttt ttggaaggct cgacgggcaa acgccacgag
     4141 ggccagcagc cccaagacca gacagatcca cggcaaggca atacccagcc agacgggggt
     4201 catgtcgcgg tcctttccgg acgcttaggc tgaagcgtgg ccgagaggct ccgaaatcgc
     4261 ggcttgcggg tgaggtacca aacatcggtg atgacggccg caagagccag gaccgcgagc
     4321 aaccacgacc attcggtatg cgattgggaa acctgctccg cggtggtcgt cttcacatcc
     4381 gaagacgtga attcgagcgg cttgtcccga acgtcgcttt cattctccga ggtcaagttg
     4441 atcgcaaata ccaccgaacc aggaatcggt tttccccatg atgcatggtt aattccagcc
     4501 ttttgcgtgc tgggcacaat cgcaagcccg tcgcgcgcgc gcagcgattg cgtgacttcc
     4561 ccagggccca cgaccttcac ttcttgcacg tgatgcggaa ccgccagccg aacaggttcc
     4621 cccgcttttc ccgctcccac aaccccatgg gagcggtggg tgcgcgccaa ctccacgaga
     4681 tttcgcacaa ataacacgaa cgacgccttg tacggccaat tggtatcccc cacatcaaac
     4741 cccaccagcg tcaccgtccg cccaggtaac gatacatccg ccgctatcgc cccctcccgt
     4801 gcatgaatga gttcatgacg cggattgtcg acccccaaca gacgagcctt ttccatcaat
     4861 acgccgtcca gcgtgagaaa tcgaaaacgc tgatcggccg tcgcccacga cgtaatcatc
     4921 ggtttgtcga cgagcccctt caccgtcgtc gtcaggcaag gcccctcggg aggattgagg
     4981 atgagcatat cgccgggggg caaaaccgta ggacattggc cttcgatgac gaccagcgct
     5041 cccgggggaa tcgcaccgga ttccaggtcc gaaacgcttc cttcttccca cgctacattc
     5101 ggatccgaac gaaaggcgcg ttcgatccag ggcgatcgct tacccagccc gatgaccgaa
     5161 atttcttcac caggaggaac ccgagcgtag gccacatcat cgaccggcat ggcgtcggca
     5221 ggcgacaatt gcacgagcaa gccttgccca atatcgccag gaaccgcttc gaaggacaat
     5281 accacgggag ctttttcccc tggctgcaac aagacctttc gtgatgcaag cacgtccgaa
     5341 gcgttgtgtt gacgaagcgt gacgaac
//
LOCUS       DAOSOR010000415         5365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15383_length_5365_cov_4.364783, whole genome shotgun sequence.
ACCESSION   DAOSOR010000415 DAOSOR010000000
VERSION     DAOSOR010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15383_length_5365_cov_4.364783"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1941
                     /locus_tag="PK710_18525"
     CDS             <1..1941
                     /locus_tag="PK710_18525"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOT11779.1"
                     /translation="VVGADLLVNVWGYCLADPRTDRQIPRGLLHPRRIRDGVHRGVID
                     GGNQSGIPYGRGWELFDDRFLGKPLVFCGTVGKMPVMSAGRPTHLKAARPGDRIVMCG
                     GRIGKDGIHGATFSSAQLDESSPVQAVQIGDPITQKIMFDFLIEARDRGLYHAITDNG
                     AGGLSSSVGEMCVASGGAQLDLAKAPLKYPGLDPWEILISEAQERMTLAVAPEHLEAF
                     MDLARHRNVEASDLGVFTDSGYFHVKYADDTVVYLPLAFLHDGLPAMRLTARWQPPRH
                     EEPLLAERKDYSEVLVSLLGQPNLASGEEKARHYDHEVKGLSVIKPFVGVRGDMPSDA
                     AVFLVSHGSFDGVVLSEGVHPHYADIDAYWATAAAVDEAVRKAVGTGARLDHMAGLDN
                     YCWPDPVQSDITPDGQHKLAQLVRSATALAKACETYGVPCISGKDSMKNDSMMGGVKI
                     SIPPTLLFSVIAKIDDVTKAVSMDVKTVGAKVLLLGATRDELGGSEYFRMLGAVGNAV
                     PKVYFEETLPLYRAVQTAIENELLLSCHAVTRGGLAVALARSAMAGRLGLVLDLDEDE
                     RLRSLTTPAALFSESTGRFVVTVDPSKIKQLEAILPSGSHSLLGVTTPDAMVEVKHRG
                     NKVLQTAVDTLVHAFQETFHAI"
     gene            1931..2761
                     /locus_tag="PK710_18530"
     CDS             1931..2761
                     /locus_tag="PK710_18530"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOT11780.1"
                     /translation="MRSDVEARRNNVRVLVLTGFGLNCEAETTHGFRLCGAQVEQKHL
                     SDVLLSKRPLTDYHIIAMVGGFSFGDHIAAGVVWANRLRYHLFDRIMRFVSEGGLMLG
                     VCNGFQTMVKLGLLPGFDRGYGQPVATVAANDRLGYRNAWVRVKVDLESPCVWTRGID
                     EMELPARHGEGKFLLQNNELLDRLRDEHLVALRYVDEQGKPTQRWPDNPNGSPDAVAG
                     VCDPTGRLLGLMPHPDAYLYGINHPRWARKKLEGTLQREGQGLRVFQNGVDYAADLKN
                     "
     gene            complement(2780..3784)
                     /locus_tag="PK710_18535"
     CDS             complement(2780..3784)
                     /locus_tag="PK710_18535"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT11781.1"
                     /translation="MRWILRFVVLLVFVGCDQRPPQPHQEPSAEPSPPENPACARHGE
                     SPFCSDHEIMQCQKGVPTVLSKCSTIERCDKGKCVANCPEGEVFIPATGPDGFTMGRD
                     RLKGKDTPHTVVLTRPFCMDETEVTSGAYAKCVQEGACAEPYRWDPWASYPRFPDQPV
                     NLVNLNKAEAYCAWKGKRIPTEAQWEWAASGGESIEWPWGNEEPTCENQLMDFTPFGA
                     PKSAPGGDVGCRGGGPSDVKTFPRGAKVWPSGKIYDLAGNVWEWTRDMSAPYPKEKQV
                     DPEIKEIPGKPDNTVYVIRGGGWNRSALGCTVWFRAEATRNYQVPGLGFRCIREAEPR
                     "
     gene            complement(3854..4984)
                     /locus_tag="PK710_18540"
     CDS             complement(3854..4984)
                     /locus_tag="PK710_18540"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOT11782.1"
                     /translation="MRRPMLFFMLLGALVHCEEKKQPPTASAPSVTTLPAASLSASAS
                     AIAAVAQPPRPVVLVAGGDIDLSRNTGQRILKNPELNPFSNLQSWLRAADIRFANLES
                     PLCDLGGKTVSEHNRLIFAGPPSGADVLARGHFDIVSTANNHIWDFGKRCLEETLFHV
                     KRVGIQAVGTDAQGKDPLRPVILERNGQHFAFFAVTGIFNHGRLRDHVAKPYIADADM
                     GALSQRITEVKPKVDWVIVSVHIGEEYIHIPIEATRYALIGAAQAGADLVLGHHSHTP
                     QRVEFHKQIPVVMSLGNFVFHQHRDHPWTGWSYLARVTFPPKGPLTIEACPFFIFDAS
                     PQPLTQAQQTAYFQHWDAISRAPRAAIRGARSADGCVALLSP"
BASE COUNT         1102 a         1401 c         1708 g         1154 t
ORIGIN      
        1 gtcgtgggtg ctgacctgct cgtcaatgta tggggctatt gcctcgccga tccccgtacg
       61 gataggcaga ttcctcgcgg gcttctgcac ccacgacgta tccgcgatgg agttcatcgc
      121 ggcgtgatcg atggtggaaa tcaatcggga attccttatg gacgcggctg ggagctattc
      181 gacgatcgat ttttgggcaa acccctcgtg ttttgcggaa ccgtcggcaa gatgcccgtg
      241 atgagcgccg gtcgtccgac gcacctcaag gcggctcgtc ccggagatag gatcgtgatg
      301 tgtggggggc gcatcggcaa agatggcatt cacggtgcca cattttcttc cgctcaattg
      361 gatgagtcgt cgccggtgca agccgtgcag atcggcgatc cgatcacgca aaaaatcatg
      421 ttcgattttc tgatcgaagc gcgggatcga ggcttgtatc atgcgataac ggataatggg
      481 gcgggaggat tgagttcgtc ggtgggagag atgtgtgtgg cgagtggtgg cgcgcagctc
      541 gatcttgcga aagctccgct caagtatccg ggactcgacc cttgggaaat cctgatatcc
      601 gaagcccagg agcgcatgac cctcgcggtg gcgcccgaac acctcgaagc gttcatggac
      661 ctggctcgac atcgcaacgt ggaggcctcc gatctggggg tcttcaccga ttccggctat
      721 tttcacgtga aatacgctga cgataccgtg gtgtacttgc cgttggcgtt cttgcacgat
      781 ggcctgccag ccatgcgatt gacggcccgt tggcagcctc cgcgtcacga agagcctttg
      841 ctcgccgaac ggaaggatta ttccgaggtc ttggtttctt tgctcgggca acccaacttg
      901 gcatcgggtg aggaaaaggc tcgccattat gaccacgagg tcaagggatt gtccgttatc
      961 aagccgttcg tgggggttcg aggcgacatg ccttcggatg cggcggtctt tctcgtttct
     1021 catggaagct tcgacggggt ggtgctttcc gaaggggtgc atccccacta tgccgatatc
     1081 gatgcgtatt gggcaaccgc cgcggcggtc gatgaagccg ttcgaaaagc ggtcggaacc
     1141 ggcgcacgcc tcgaccacat ggcgggcttg gacaactatt gctggcccga tccggtgcaa
     1201 agcgacatca cgcccgatgg acaacacaag ctcgcccagt tggtgcgctc ggccaccgcc
     1261 ctcgccaaag cctgcgaaac gtacggtgtg ccttgtattt ccggaaaaga ctccatgaaa
     1321 aacgactcga tgatgggagg tgtcaagatc tcaatcccac caaccctgct tttcagcgtc
     1381 atcgcgaaaa ttgacgatgt taccaaggcc gtgtcgatgg atgtgaagac cgtcggagcg
     1441 aaagtgctgc ttttaggtgc cacgcgcgat gaactcggcg gctcggagta tttccggatg
     1501 ctgggcgcgg tgggaaatgc cgtgcccaaa gtctatttcg aagaaaccct accgctgtac
     1561 cgcgccgtgc agacggccat cgaaaacgag ttattgctct cttgtcatgc cgtcacccga
     1621 ggtggcctcg cggtggccct cgcacggtcg gccatggctg ggagactcgg ccttgtcctg
     1681 gacctggatg aggatgagcg cttgcgatcg ttgacgaccc ctgccgctct cttttcggaa
     1741 agcaccggaa ggttcgtggt gaccgtggat ccatcgaaaa taaagcaact tgaagcgatt
     1801 ttgccctcgg gctcccacag cctgctcggg gttacgaccc ctgacgccat ggtggaggtg
     1861 aaacaccgtg gcaataaggt gctccaaacc gccgtggata ccttggttca tgccttccag
     1921 gagacgttcc atgcgatctg atgtcgaagc gcggcgaaac aatgtccgag tgctcgtgct
     1981 gacggggttc gggctcaact gcgaagcgga aacaacccac ggatttcggt tgtgtggggc
     2041 tcaggtggag cagaagcatc tatcggatgt gctcctgtcg aaacgccccc ttactgacta
     2101 ccacatcatc gccatggtgg gcggattctc tttcggagac catattgccg cgggtgtcgt
     2161 atgggcgaat cgattgcgct accacctatt cgaccgaatc atgcggttcg tgagcgaagg
     2221 gggattgatg ctcggtgtct gcaacggctt tcagaccatg gtcaagcttg ggctcttgcc
     2281 gggcttcgac agggggtatg gtcagcccgt ggcgacggtc gctgccaatg atcgattggg
     2341 gtaccgaaac gcgtgggtga gggtaaaggt ggatctggaa tcaccgtgtg tgtggacccg
     2401 gggaatcgat gaaatggaac tgcccgcacg gcatggagag ggaaaattcc ttttacaaaa
     2461 caatgagttg ctagatcgtc ttcgagatga gcatctggtg gccctgcgtt atgtggacga
     2521 acaaggcaag ccgacacaaa ggtggccgga taatccaaac ggttcgccgg atgcggtggc
     2581 gggcgtttgt gatcccacgg gcaggttatt ggggctgatg cctcatcccg acgcgtattt
     2641 gtacgggatc aaccatcccc gctgggcgag aaagaagctg gaaggtacgc ttcagcgtga
     2701 ggggcaaggg ttgcgggtgt ttcagaacgg ggttgattat gcggcggatt tgaagaattg
     2761 atggcggttg gccgcaggat caccgcggtt cggcttcgcg aatgcaccga aagccgagtc
     2821 ctggaacctg atagtttcgt gtggcttccg cgcgaaacca tacggtgcat cccagggccg
     2881 agcgattcca accgcctccg cggatcacgt acaccgtatt atcaggtttg cccggtattt
     2941 ctttgatctc tggatcgact tgtttttctt tgggatatgg cgctgacata tctcgtgtcc
     3001 attcccagac attgcccgca agatcgtaga tttttcccga cggccagact ttggcgccac
     3061 ggggaaaggt tttgacgtcg gagggaccgc cgccgcggca ccccacgtcg cctcccgggg
     3121 cggatttggg agcgccgaag ggtgtgaagt ccatgagttg attttcgcac gttggctctt
     3181 cgttgcccca gggccattcg atactttccc cgccactggc ggcccattcc cattgggctt
     3241 cggtggggat tcttttgcct ttccaggcgc agtaggcctc cgctttattc aaattcacaa
     3301 gattgactgg ttgatctgga aatcgaggat agcttgccca cggatcccat cggtaaggtt
     3361 ccgcgcaagc accttcctgc acgcattttg cgtaagcgcc cgaggtgact tcggtctcgt
     3421 ccatacaaaa cggccgggtg agcaccacgg tatgaggcgt gtcttttcct ttgagacgat
     3481 cacgacccat cgtgaatccg tcggggccgg tggcgggaat gaatacctct ccttcggggc
     3541 aatttgctac gcattttcct ttatcacagc gttcgatcgt ggagcatttg gaaagaactg
     3601 taggaacccc cttctgacac tgcatgatct cgtggtccga gcaaaacggc gactcgccat
     3661 gccgcgcaca agcggggttt tctgggggcg acggttcggc cgagggctcc tggtgtggtt
     3721 gcggcgggcg ttgatcgcag ccgacaaaaa caagcagaac gacgaaccga agaatccaac
     3781 gcatcgaaag tgaatcgtag tcgcgggaag tccaaacggc caagtcacgg tgggtggtgg
     3841 gtatttcgat gagtcacggc gaaagcaggg ctacgcatcc gtcggcggaa cgcgcgccgc
     3901 gaatggctgc cctgggggcg cgggagatgg catcccagtg ctggaaataa gcggtctgct
     3961 gagcttgcgt gaggggttgg ggcgaggcat cgaagatgaa aaacgggcag gcttcgatgg
     4021 tcaaggggcc tttgggggga aaggttaccc gtgcgagata gctccagccg gtccacgggt
     4081 ggtcgcgatg ctggtgaaat acgaaattcc caagactcat caccaccgga atttgtttat
     4141 gaaattccac acgttggggc gtgtggctgt ggtgcccgag caccagatcc gcgcccgcct
     4201 gggcggcccc gatgagagcg taacgggtgg cttcgatcgg aatgtgaata tattcctcgc
     4261 cgatatgcac cgagacgatg acccaatcga cctttggttt cacttcagtg atacgctggg
     4321 acaacgcgcc catatcggca tcggcgatat agggtttggc cacatggtct ctcagacgac
     4381 cgtggttgaa aataccagtt accgcaaaaa acgcgaaatg ctgcccattt cgctcgagga
     4441 tgacgggccg cagcgggtct ttgccttgcg cgtcggtacc caccgcttga atgcccacgc
     4501 gcttcacatg aaacaaggtc tcttcgaggc agcgctttcc gaagtcccaa atatggttgt
     4561 tggcagtgga tacgatatcg aaatggccgc gcgccagaac gtcggctcca gaaggggggc
     4621 ccgcgaagat gaggcgattg tgttcggaaa cggttttccc gccgagatcg cataacggcg
     4681 attcgaggtt ggcaaagcgg atatccgcgg cgcgcagcca ggattgaagg ttggaaaagg
     4741 gattcagctc gggatttttc aaaatgcgct ggccggtatt ccgagacaga tcgatatcgc
     4801 cgccggcgac aaggacaacg ggccgaggcg gttgggcaac ggctgcgatg gcgctggcgg
     4861 atgcgctgag agacgcggca gggagcgttg taacactggg cgcggaggca gtcgggggtt
     4921 gttttttctc ctcgcaatga accaatgccc cgagcagcat gaaaaatagc attggtcttc
     4981 gcatggttgg catcagcttg tcagaatgga ggcgaaacgc aatgattggc cgggggcaac
     5041 gagaccccag tccgggctcg gtatttgtct aactatttga aataacttat gaataacagg
     5101 ctccctcggg ccttttccca aggcggatga agccaccgaa cgcggctgtc gcttgaccga
     5161 ccgcagatga gcgggtcaaa ctgtccagat gcgaaaaatc ggttggcttt gcattcctgt
     5221 agggttggtg gtggcgtgtt ctggcagtga agacagcgca caagataatg gtggagtagg
     5281 aggcaccacg acagacgggg gtgttgaggc cgcgcagggc gggacgggag cgggggccgt
     5341 gacgggagga atgggaggag cgggc
//
LOCUS       DAOSOR010000416         5357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15409_length_5357_cov_4.375707, whole genome shotgun sequence.
ACCESSION   DAOSOR010000416 DAOSOR010000000
VERSION     DAOSOR010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15409_length_5357_cov_4.375707"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PK710_18545"
     CDS             complement(<1..396)
                     /locus_tag="PK710_18545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11783.1"
                     /translation="MGWIVGTEPRARRAPIDSIPGIFSYPYCMMAFRKPWHVVALAAT
                     VYFATSVTGCTQDGPVAQSESEAASPMRLPTSLPGWEPSSVLEVAPDGRAIIGKELAV
                     PPRSTARQVYEAAFIDEKGNPQVLIVLSEK"
     gene            471..1472
                     /locus_tag="PK710_18550"
     CDS             471..1472
                     /locus_tag="PK710_18550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11784.1"
                     /translation="MRRRWYAATIAVALVLLDVAAWAEVVLMLPPSGQGVQESFAGHL
                     RKDSRLAVVELGHQLVAEEQEVAALRTISDGLADDATEFETLAHHTHANWVVSPVVHL
                     TDGGVRLELTAFLASTGRCETVARELDRDSLQPQVREMLAVLLRQEGVGTGALPWEKS
                     PSALRSSPPGEKRIETEQTASSTQEPSRSFMPLLGATFGGSTTLARPEQAVGSSTAWQ
                     GGVRAGIRFGEALELALGLRSNIAGPKATGFDLSARYGFRISEGIRVGPELAPGGFVQ
                     GGGAQRTSLSLRFSLVGSLDITPHISIEAQAGDITWVATGEESLFLGGATLAAVARL"
     gene            1534..2286
                     /locus_tag="PK710_18555"
     CDS             1534..2286
                     /locus_tag="PK710_18555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11785.1"
                     /translation="MRELLMIAAMALVGCGSAPSPSKAPASKASETPAPTNEKAEVPT
                     PETAEESPHVDVPTSCDQGVDKPCVMPRAFVKQLCAGAFPELALFFFAKGTPWTRVYV
                     AVRQAEPFNGLGGPSSDKNLEFDEELLVLSENTPNLGGMSVSGVGNSYDVLRWDGTCA
                     TLQAGEVRLQRPPQPKHADVDWKRLDEEVRDALSADGTIADLAHRRRQECKGVTMGVV
                     SDKCEKADTALRARVVKLIREGFALPRPSRVP"
     gene            complement(2297..2878)
                     /locus_tag="PK710_18560"
     CDS             complement(2297..2878)
                     /locus_tag="PK710_18560"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOT11786.1"
                     /translation="MASKEPNEINGSVSRTDTMEQEYVLLESFPTPHDAHLVANLLRT
                     VDIPAKILDESIVSVLPILEMALGGVKVMVPRNHLEEARQLWLDDRNSLAQGSGTETQ
                     QGQPGEETSDDHADDMRLAAKEDTITRAFYASVIGLFFCPLPAHLYSAWVLLRADREG
                     TSRRTRIKMAVAWCINLAVFAVAVAMLLRAVAS"
     gene            3067..3927
                     /locus_tag="PK710_18565"
     CDS             3067..3927
                     /locus_tag="PK710_18565"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT11787.1"
                     /translation="METHTLTTSDGLRLHAETIGRGPSMLMCNGLFCTTQYFVSWREH
                     FGQSYRLVQFDYRSHGRSDDDPEPSRVTIERLVDDAALVLEKLCDEPTIVVGHSMGVR
                     VALELYRRHPERISTLLLLCGSVFDSLGGVASLPPVRHAVVRVLDSAGWLGPLARGIK
                     DVAVHQDLVSKVGTALGGLSAKTPRAPVDALLAHLDRLDVRMMVSLARSYIHHSSEDI
                     LPRVKVPTLFMVGQKDSLAPPSHARKVAALLPDVQQVVIQGCTHLAPVEKPDEVHQVV
                     ERFLARISRR"
     gene            complement(4018..>5357)
                     /locus_tag="PK710_18570"
     CDS             complement(4018..>5357)
                     /locus_tag="PK710_18570"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT11788.1"
                     /translation="PQVSFTLQALYRTLDPKPQKLLVMARASAAATIQDDAEPSSSVQ
                     LAVAVDISGSMEGVKLRAAVSTLHALIDELGPNDSLGLIGFSNSSKVWLPSTPMTSAA
                     KQIAHACLDRAKADGGTDLAGAILSALRVIEASSERRKHVIVLTDGQPTAGVCDPDRI
                     VDLVKKAKKDVTASFFGYGEDVNPDLLERLAQVGSGRFHFVPGTEPPVEAFAAELGQQ
                     RDLLGIDASISLTTGKGVKLCYLPSLAEVRKKKTGIRISIPNLLAGDARSVVFEIEVN
                     EDAFALSETTPWVEATLRYRSVLDAQEYVNDTHLVPVIAAEKSEPVVDVAREFSVQRL
                     AELVGAVTRWDNKNEQEKASLMDFVQRMGLQSDPQIMAGFDMLDRLQHDLADASKRRW
                     THLASSAASKGMYERSVTGMGVAHVYSKPRAESILRRMFQMVKDDDKDPEDAN"
BASE COUNT         1125 a         1520 c         1556 g         1156 t
ORIGIN      
        1 cttttcgctt aatactatta aaacttgagg atttcctttt tcatcgatga acgcggcctc
       61 gtagacttga cgagcggtag accgtggagg gacagccaac tctttgccga tgatggcccg
      121 accgtctggc gccacttcca gcacggaaga tggctcccat cctggcaatg acgttggaag
      181 ccgcatcgga gaagcagctt cggattcgga ctgcgcaacg ggaccatctt gcgtgcatcc
      241 cgtcacgctt gtcgcaaaat agaccgttgc ggcgagagcc accacatgcc aaggcttccg
      301 aaaggccatc atgcagtaag gatagctgaa aataccgggg atggaatcga tgggggcgcg
      361 tcgcgcacgc ggctccgtac cgacaatcca ccccattggt gaaacgtctt gcttgccccc
      421 gcgaaatcaa ccccctcatg gcccgtccga tgctacggaa gacagtcacg atgagacgac
      481 gatggtatgc ggctactatt gcggtggcgt tggtattgct cgatgttgcg gcttgggcgg
      541 aagtcgtgtt gatgcttcca ccctccggac agggagttca ggagtcgttt gccgggcact
      601 tgcgcaagga tagtcgcctg gccgtggtgg aactgggaca tcaactcgtg gcagaagagc
      661 aagaggtagc cgctctgcgc accatttccg atggcttggc ggatgatgcg actgaattcg
      721 aaactctggc ccatcatacg cacgccaatt gggtggtgtc cccggtcgtt cacctcacgg
      781 acggaggagt tcgtcttgaa ttgaccgcct ttctggcctc gactggacga tgtgaaaccg
      841 tggcccgcga actcgatcgc gattcgttgc agccgcaagt gcgtgaaatg ctcgccgtat
      901 tgttgcggca agaaggggta ggaacgggtg cgcttccctg ggaaaaaagt ccctcggccc
      961 ttcggtcttc cccaccgggc gaaaagcgga tcgaaacaga gcagactgcc tcctcgactc
     1021 aagaaccatc acgaagcttc atgccgttgc ttggagcgac attcggcggc tcgacgacct
     1081 tggctcgccc ggagcaagcg gtcggcagct ctacggcgtg gcagggagga gtccgagctg
     1141 ggattcgctt tggcgaggcc ctcgagttgg ctttgggatt gcgttccaac atagcagggc
     1201 ccaaagcgac gggattcgat ctgtccgcac gctacggttt tcgcatctcc gaggggattc
     1261 gagtcggtcc ggaactggct ccaggcggat tcgtacaggg cggaggcgcg caacgaacgt
     1321 cgctttcgct tcgattctcc ttggtgggtt ctttggatat taccccccac atttccatcg
     1381 aagcgcaagc aggagacatt acctgggtag ccacgggtga agaatccctc tttctaggcg
     1441 gcgccacgct ggctgctgtc gcaagactct gaacggcttt cgatcgccac gcgcacgttg
     1501 cattccacgc cagcttgcgg tttcatcccc cccatgcgtg aattgctgat gatcgccgcc
     1561 atggcccttg tgggatgcgg ttccgctccc tccccttcga aggctcctgc atccaaggcg
     1621 tcggaaacac ctgcgcccac gaacgaaaag gcagaggttc ccacgccgga aaccgcggag
     1681 gaaagcccgc atgtggacgt gccgacgtcg tgcgatcagg gagtcgacaa accgtgcgtg
     1741 atgccccgcg ccttcgtgaa acaattgtgc gcgggcgcat ttcccgaatt ggcgctcttc
     1801 ttctttgcga aaggcacacc gtggacgcgt gtctacgtgg ccgtccggca ggccgaaccc
     1861 ttcaacggat tgggagggcc gtcgtcggat aaaaacctcg agttcgatga agaactgctc
     1921 gtattatcgg aaaacacacc caatctcggc gggatgagtg tcagcggcgt gggcaatagc
     1981 tatgacgtgc tccgatggga tggcacctgc gccacattgc aggcgggaga agtacgattg
     2041 cagcggcctc ctcaacccaa acatgccgat gtcgactgga agcgactgga cgaagaggtg
     2101 cgcgatgcgc tgagcgcgga tggcacgatc gcggatctgg cgcaccgtcg acgacaagaa
     2161 tgcaagggcg tgaccatggg tgtcgtatcc gacaaatgcg aaaaagccga tacagcgctt
     2221 cgagcacgcg tggtgaaact catccgtgaa gggtttgcct tgccgcgccc ctctcgcgtt
     2281 ccatgaatgg agcaggttag ctggctaccg cccgcaacag catcgccacc gcaaccgcga
     2341 atacggcgag attgatacac catgccacgg ccattttgat ccgggttcga cgactcgtgc
     2401 cttcgcgatc ggcgcgcagt agcacccaag cggaatagag gtgagccggc aaaggacaga
     2461 agaacaggcc gataaccgat gcgtaaaacg cgcgcgtgat cgtatcctcc ttggcggcca
     2521 gacgcatatc gtccgcgtga tcgtcggagg tttcctcccc gggttgcccc tgctgcgttt
     2581 cggtgccgct cccttgggcc aacgagttac gatcgtcgag ccatagctgt cgtgcctctt
     2641 cgagatggtt tcgaggaacc atcactttca caccaccgag cgccatctcg agaatgggaa
     2701 gaacgctgac gatgctttcg tcgaggattt tcgccggaat atcgacagtg cgcagaaggt
     2761 tcgccacgag gtgcgcatcg tgcggcgtgg ggaacgactc cagcaataca tattcctgtt
     2821 ccatggtatc cgtcctcgac acggaaccgt tgatttcatt gggttctttg gacgccatct
     2881 aacgattttc cctggtccgt ggatttcgtt cgccggatta accgaaacga ggaggcaact
     2941 cgtgcttacc ccacaaacgg cgatccagtg gcaaagaaat gtcggtcaag atgcccatga
     3001 acggaccttc atgccatcgt gttccgtctg cgtagcgctc agacaacgtg atataggggc
     3061 cttatcgtgg aaacccatac cctaaccaca tcggatggtc tccgactcca tgcggaaacg
     3121 atcggccgtg gaccgagcat gttgatgtgc aacggtctat tttgcaccac gcagtacttc
     3181 gtttcgtggc gagaacactt tggtcaatcc tatcggttgg tacaattcga ctatcgtagt
     3241 catgggcgaa gcgacgatga cccggagcca tcgagggtta ccatcgaacg attggtggat
     3301 gatgcggcgt tggttctcga gaagctttgc gacgaaccga cgattgtcgt tgggcattcg
     3361 atgggggtga gggtggccct ggaactctat cgtcggcatc ccgagcgcat ttccacgctg
     3421 ctgcttctgt gcggcagcgt tttcgattcg ttgggaggag ttgccagcct gccgcctgtt
     3481 cgacatgcgg tggtgcgcgt gctcgattcc gcgggttggt taggaccgct ggcgagagga
     3541 atcaaggatg tggcagtgca tcaggacctc gtatcgaaag taggaacggc ccttggtggg
     3601 ttgagcgcga aaacgcctcg agccccagtg gatgccttgc tcgcccattt ggatcggctc
     3661 gatgttcgaa tgatggtttc cctcgcccgg tcgtatatcc accattcatc cgaagatatt
     3721 cttcctcggg tcaaggtccc cacgcttttt atggtggggc aaaaagatag tcttgcaccg
     3781 ccttcccatg cgcggaaagt tgccgccctt cttcccgatg ttcagcaagt cgtcatccag
     3841 ggatgcacgc accttgcgcc agtggaaaaa cccgatgagg tgcatcaggt ggtagaacga
     3901 tttcttgcgc gaatcagccg tcgatgaagg gaccgccggg aacgtcacct cccgaaaaac
     3961 gcgtaaacta ttacaattat tggtattttt cgaaaaccgt ctcccgcacg gcattcgtca
     4021 gttggcgtct tccggatcct tgtcgtcatc tttcaccatc tgaaacatcc gtcttaggat
     4081 cgattcagct cgtggcttcg agtagacgtg cgcaactccc atgcccgtga ccgaacgctc
     4141 gtacattcct ttggatgccg cgctggatgc aagatgggtc catcttcgct tcgaagcgtc
     4201 ggccagatcg tgttgaagac gatccaacat gtcaaatcca gccatgatct gtgggtccga
     4261 ttgaagtccc atgcgttgga cgaagtccat aagggacgcc ttttcctgct catttttatt
     4321 gtcccatcgc gtcacggcgc ccacgagttc cgcaagtcgt tgtacggaaa attcccgggc
     4381 gacatccacg acaggctcgc ttttctcggc ggcaatcacg ggcacgagat gcgtgtcatt
     4441 gacatactct tgggcatcga ggaccgaacg gtaacgaagt gtggcttcga cccaaggcgt
     4501 ggtttcggac aaggcaaagg catcttcgtt tacctcgatt tcgaacacca cgcttcgcgc
     4561 atcgccagcg agcaagttgg gtatgctgat gcgtatgccc gtcttcttct tccgcacttc
     4621 agccaaagaa ggaagataac agagtttcac tccctttccc gtcgtcaggc tgatcgaagc
     4681 atcgatgcca agaagatccc gctgttgtcc aagctcggca gcaaacgcct ccacgggagg
     4741 ctccgtccca ggtacgaagt gaaaacgtcc ggagccaacc tgcgccagac gctcgagcaa
     4801 atcggggttg acgtcttccc catacccaaa aaaactcgcg gtcacgtctt ttttggcttt
     4861 tttcacaaga tcaacgatcc tgtccgggtc gcagacgccg gccgtgggct gaccgtcggt
     4921 aagcacaatg acgtgctttc ggcgctccga actcgcttcg atgacgcgaa gagccgaaag
     4981 gatggcgccg gccaaatccg ttcctccatc ggctttggcg cgatcgagac aagcgtgggc
     5041 gatctgtttt gcggccgaag tcatgggcgt ggaaggaagc cataccttgc tcgaattaga
     5101 aaaaccgatg agtccgagag aatcattggg tcccaattca tcgatgagag cgtgcagggt
     5161 cgagaccgcg gcgcgcaatt tgacgccttc catcgacccc gatatgtcaa cggcaaccgc
     5221 aagttgaacc gatgacgaag gctctgcatc gtcttggatc gtagcggcgg ccgatgctcg
     5281 ggccatgacg agcaactttt gcggctttgg gtccaaggtt cgatacagag cctgcaacgt
     5341 gaaagaaacc tgtggct
//
LOCUS       DAOSOR010000417         5351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15437_length_5351_cov_3.518882, whole genome shotgun sequence.
ACCESSION   DAOSOR010000417 DAOSOR010000000
VERSION     DAOSOR010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5351
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15437_length_5351_cov_3.518882"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="PK710_18575"
     CDS             complement(<1..1248)
                     /locus_tag="PK710_18575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOT11789.1"
                     /translation="MRLPHVFTLLTLLGSLAACDRGSKTGQTLASNSATTSQPESVDS
                     LGNPVFLQRDTPPEVSLSGASSAQHLSLPGSSNRLAALAWETPIYKLPDKDSVLLGYL
                     RVGAIVAASAKPVGYKDCPAGWYQIAPFGHVCIAPSNATLDLQHELVRATAQRPDVTN
                     PLPYIYGIVRKPGPIYSKLPSLQEASSAESDLGSRMAEWLELSGPNGASFRTDLWLRW
                     KDPTQVPTPQSLWESKANLHIPWFLDGRSPPGNLANLGKNKNDLVVGQMRKHQGFAFI
                     DTAVRDGRRYGITTRLLSIPVDRVRPIAGSSFHGFEIPSDIDFPFALVRARNAFAYRI
                     DNGKRTKANPLAHRSAIALVDKKTFHGGVLHYQTADGFWVSDREVSRLDPARRMPAWG
                     KDGERWIDINITKQTLVAYDGTKP"
     gene            complement(1551..2549)
                     /locus_tag="PK710_18580"
     CDS             complement(1551..2549)
                     /locus_tag="PK710_18580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HOT11790.1"
                     /translation="MHTLDPTLDIVFKLLFGGPKSSDILIALLGAVLQPQSPITEVSI
                     LNPAVPKQTTEEKDIVLDLLVRLQDGTLVNIEMQAENRPGFRSRALYYWARVYADQLH
                     RGEDYEKLKPVVMIVFTGYRENITRRLHAVFHVLEIHEHVRFQQGFELHLIELPRIKE
                     PQAMATARAAMRRWARFLGANTDEERESVAKEDPMVQQAQKILMEISSDEDVQKLLRE
                     RDVARKFLEMDLVLATERAEAKGWKRGRKKGREEGRKEGRKEGRKEGRQQGREEGRRE
                     AIMQLLSLRGIELTPADHSALMACNHMETLDKLLQRALTLPPDESLFDGIDVQSKP"
     gene            complement(2806..3465)
                     /locus_tag="PK710_18585"
     CDS             complement(2806..3465)
                     /locus_tag="PK710_18585"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOT11791.1"
                     /translation="MDSRQIITYWILCFVSIFAVINPFRATTWTAMATEGWSEEQRKR
                     AVHRALLVASAVLFCAAWIGNHVIIRSNIHLGGFRVASGILLLTTVIRRMVLNRPFDL
                     QQVKGISEDRALELGFAPLAFPLLSGIAAIATVILYTGEPTELWRRGTTLVALGLTLV
                     LAWGMMRAAPRIHAFWGEQGSRIITHGLDLTIAAWAVDFTAVGVRDLLPLILGTPPAG
                     G"
     gene            complement(3481..4452)
                     /locus_tag="PK710_18590"
     CDS             complement(3481..4452)
                     /locus_tag="PK710_18590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11792.1"
                     /translation="MMKNNACVSWILWANVVLLGCGGSAGFAKGGDPSFGTELETGAF
                     AETRQVAEEHGEHGKPSQGGAEHITVASGPIVVDHTSVNAFASIPPPVIEKAKQQLAF
                     FYGHLSHGEQLLNGMDILASEKPLYAYQKSFLQPFVSSLDPRPQFPQWEPATREQLTA
                     AINDRNVVMWAWSSWLGSPDEVDEAYVSEYLTKMERLETDFPAVRFVYFTGPAQTWVD
                     PQRSMAKRNAQIREYCKASDKILFDIEDIELHAPDGTYYAKGTHACEWCTTWCASHDC
                     SPIVPPSCGACDDRCGACRGNHTHCFNCYRKGQAFWWLAARLVGWNG"
     gene            4598..>5351
                     /locus_tag="PK710_18595"
     CDS             4598..>5351
                     /locus_tag="PK710_18595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11793.1"
                     /translation="MTYRWSALVGLLFLTLSCCKKKGVEDVPLPPPAKPIEKQLVWVV
                     DDGERVARHAGALPWMAGENNAVWKPGQPIRLFGLPGETIAFQVVVTAASERIESVTV
                     DLDELSGPVTIKNDSALPATSFRPIERFVVHELEVKRRSGGKVKGESLGWKASSMPED
                     PSRDRFIPDPLIPIEVAPSWANYPMIVNPGEHRVVWIDITLPTTGLPAGSYRGSVEVR
                     SSSAALASIPVDLEVGSVALPYAAVATMVYVEP"
BASE COUNT         1179 a         1482 c         1529 g         1161 t
ORIGIN      
        1 aggcttggta ccatcgtagg cgacgagagt ttgtttggtg atgttgatat cgatccatcg
       61 ttcgccatct tttccccaag ctggcatacg acgcgcgggg tcgaggcggg atacttcacg
      121 gtcggaaacc cagaaaccat ctgcggtctg atagtgtaat accccgccat gaaaggtttt
      181 tttgtcgacc agggcaatgg ccgaacgatg ggcgagcggg ttggctttgg tgcgttttcc
      241 gttgtcgatg cggtaagcga aggcattgcg agcacgaacg agagcaaagg ggaaatcgat
      301 gtcggacgga atttcaaaac cgtgaaatga agagcccgca atggggcgaa cgcgatcgac
      361 ggggatcgat aacagtcgtg tggtgatccc atagcgtcgc ccgtcgcgaa ccgccgtatc
      421 gatgaatgca aacccttggt gttttctcat ttgcccgaca acgaggtcgt ttttgttttt
      481 tccaaggttg gcaaggttac cgggaggaga gcgaccgtcg agaaaccaag ggatatgaag
      541 gttagctttc gattcccaga gggattgggg cgtcggaacc tgggttgggt ctttccagcg
      601 aagccataag tcggtgcgaa aggaggcacc gtttggaccg gatagctcga gccattccgc
      661 catgcgggaa cctaaatccg attccgcgga tgaggcttct tgaagcgagg ggagcttgga
      721 atagatggga cctggttttc gaacgatgcc atatatgtag ggaagcggat tggtaacgtc
      781 ggggcgttgg gctgtcgcgc ggacaagttc gtgttgaagg tcgagggttg cattcgatgg
      841 cgcaatgcaa acatggccga agggagcgat ttgataccag ccggcggggc agtccttata
      901 accgacaggc ttagcactgg cggcaacgat ggctccgacc cgcaaatagc caagaaggac
      961 ggaatctttg tccggcagct tgtagatggg ggtttcccaa gcaagggcgg caagtcggtt
     1021 ggacgaaccg ggcagggaga ggtgttgggc ggaggaggcg ccgctcagag acacctcagg
     1081 tggggtgtct ctctgtaaaa acactgggtt accaagcgaa tcgaccgact cgggctggga
     1141 tgtcgttgcg gaattgctgg cgagcgtttg gccggtcttg gagcctcgat cacaagcggc
     1201 gaggcttcct aacagggtta gaagggtgaa gacgtgaggc aatcgcatgg aaggcaccgt
     1261 gcttggcctc cgcgttgacg gcggaggaga gacggatttg gctggttggg ctgattcggg
     1321 caagtaattg gccaatggca tccgtggtca aacatgggag aatcttcctg taacggaact
     1381 ggatgcaaga aatcccaacg atatcgtgat tcaggggccg aaattgcggt cgtagtgcgg
     1441 tttttgagca aggcaacggg cgcatggaat cttatgcggc gatattctcc ggatcgtgac
     1501 gaatcatgcc gcaggggaag tggcccgagc acgtcgattg tcagcggaaa ctaaggtttt
     1561 gactgcacat cgataccgtc gaacagagac tcgtccggtg gaagagtcaa ggcccgctga
     1621 agcaatttat ctaatgtttc catgtggttg caggccataa gggcgctatg gtccgccggc
     1681 gtcagttcga ttccacgaag ggaaagaagc tgcatgatcg cttccctgcg tccttcctcg
     1741 cggccctgct gccgtccctc tttcctgccc tctttcctgc cctctttcct gccctcttcc
     1801 ctgccctttt tcctgccccg cttccagccc ttggcttccg ctcgctccgt ggcaagcacc
     1861 aggtccatct ccaaaaactt cctcgctaca tcgcgttcgc gtaaaagctt ctgcacatcc
     1921 tcgtcgctcg atatctccat caggatcttc tgagcctgct gtaccatggg atcctccttc
     1981 gccaccgact ctcgctcctc atccgtattt gcaccaagaa atcttgccca gcgacgcatc
     2041 gcggcgcgcg ccgttgccat ggcctgtggc tctttgattc tgggaagttc tatcaagtgt
     2101 agctcgaagc cttgctggaa gcgaacgtgt tcatggattt ccaagacatg aaacaccgca
     2161 tgcaatctcc gcgtgatgtt ctcccgatat cccgtaaaca cgatcatcac cactggcttg
     2221 agcttctcgt agtcttcgcc ccgatggagc tgatccgcgt acacgcgagc ccagtaatac
     2281 agcgcgcggc tacgaaaccc gggacgattt tcagcctgca tctcgatgtt gaccagggtg
     2341 ccgtcctgca accggaccag caggtccagc acgatgtctt tttcttcggt cgtttgcttg
     2401 ggaacagcgg gattcaggat gctcacctcg gtgatcgggc tttgcggctg cagcacggcc
     2461 ccgagcaacg caatgaggat gtcgctgctt ttcggcccgc caaacaagag tttgaaaacg
     2521 atatcgaggg tcggatcgag ggtgtgcatg gttgccgaaa gagcaacccg cgggccaaag
     2581 cttcaagcgc gaaatcacaa gggattttcg tctatccagg tggcggaccg ccaccctttt
     2641 tcgacgggtt ttcgtcgaaa tacgaggtaa tcgtcgagaa aaaccgctgg tggcgaaccg
     2701 ccagggccaa tcggcggccg ccagcgcttg gaactggagc cctcacaatg aactcccaca
     2761 agttgttcac ggcaaaatat gctcgcccgg ccttccgaac gccatttatc cccctgcagg
     2821 tggtgttcca aggatgagcg gaagcaaatc ccgaacgcca accgcagtga agtccaccgc
     2881 ccatgccgca atcgtcagat ccaacccatg ggtgatgata cgactgcctt gttcacccca
     2941 aaaagcatgt atccgcggtg ccgcccgcat catcccccac gcaagaacaa gcgtaagccc
     3001 aagggccaca agggttgtcc cacgacgcca caactctgtc ggctcaccgg tgtacaggat
     3061 aaccgtcgct atcgcggcaa taccgctcag caaggggaac gcaaggggag caaaccccag
     3121 ttcaagcgct cgatcctccg aaattccttt tacctgttga agatcgaagg gccggttgag
     3181 aaccattcgt cgaatcacgg tcgtaagcaa aaggataccg cttgccacac gaaatcctcc
     3241 gagatggata ttcgagcgaa taatgacgtg attgccgatc caagccgcac aaaacagcac
     3301 cgcacttgcg acaagaagcg ctcgatggac agcccgtttt cgctgctcct cgctccatcc
     3361 ttcggtagcc atggccgtcc aggtcgtggc acgaaacggg ttgatcacgg caaagatcga
     3421 gacgaagcac aatatccagt aggtgatgat ttggcgtgaa tccataagtt cgctcccgca
     3481 ttacccattc cagccaacga gccgagccgc aagccaccaa aaagcttgtc ctttgcgata
     3541 acaattgaag caatgcgtgt ggtttccacg acaggcccca cagcgatcgt cacacgctcc
     3601 gcaggagggc ggcacgatcg gactgcaatc gtgcgaggcg caccatgtcg tacaccactc
     3661 acaggcgtgg gttcctttgg cgtagtaggt gccatcgggg gcatgcaatt caatatcttc
     3721 gatgtcgaaa aggattttgt ccgacgcctt gcagtactcg cggatttgcg cgtttctttt
     3781 tgccatagag cgttgcggat ccacccaggt ttgcgcggga cctgtgaagt acacaaaccg
     3841 gacggctgga aagtccgtct ccagacgctc cattttcgta agatattcgg agacatacgc
     3901 ctcatccacc tcatcggggg aaccaagcca actgctccaa gcccacatga ccacattgcg
     3961 gtcgttgatc gccgcggtaa gctgttcgcg cgtagctggc tcccattgcg gaaattgagg
     4021 acgcgggtcg agggaggaga cgaacggctg caagaatgat ttctggtaag cgtacagcgg
     4081 cttttcactc gcaagaatat ccatcccgtt gaggagttgc tctccgtgcg agagatgccc
     4141 ataaaaaaaa gcgagctgct gtttagcttt ttcaatcacg ggcggtggga tcgaagcaaa
     4201 cgcattgaca gaagtatgat caaccacgat ggggcctgat gccacggtga tgtgttctgc
     4261 gccaccttgt gatggcttcc catgctcgcc atgctcctcg gcgacttgtc gtgtctcggc
     4321 gaatgctccc gtttctaact ctgtcccaaa cgatggatcc cctccctttg cgaatcccgc
     4381 actgccccca cagcccagca aaacaacgtt tgcccagaga atccaagaca cacaagcatt
     4441 gtttttcatc atcccgttcc ttttgacaaa cccgaacgtt cgctgacgct tgccaagggt
     4501 caagggcaat ccttcgtccc gttgctacgc gtccctgtac agcgtatggc tatcgcttgc
     4561 gacggatcgt ccgagatccg aaacggagag agaaccggtg acatatcgtt ggtcagccct
     4621 ggtcggtttg cttttcttga cgttatcttg ctgcaaaaag aaaggggtcg aagacgtccc
     4681 tttaccgccc ccggccaagc ccatagaaaa gcaactcgtg tgggtcgtgg atgatggtga
     4741 acgcgtggcg cgccatgccg gagccctgcc ttggatggca ggtgaaaata atgctgtctg
     4801 gaagcccggc cagcccatcc gcctgtttgg tctgcccggc gagaccatcg cttttcaagt
     4861 cgttgtgacg gcggcctccg aacgcatcga atcggtgact gtcgacctgg acgaactctc
     4921 gggtcctgtc acgataaaaa acgatagcgc attacctgcc acttcattcc gtcccatcga
     4981 acgcttcgtc gtgcatgaac tcgaggtgaa gcgacgatcg ggcggaaaag taaaaggcga
     5041 atcgctcggg tggaaagcct cgtcgatgcc cgaagatccg tctcgagatc gattcatccc
     5101 cgatcccctc attcccatcg aggtagcccc gtcctgggcg aattatccca tgatcgtcaa
     5161 tcctggggag catcgtgtgg tgtggatcga catcacgttg cccaccacgg gattaccggc
     5221 aggttcgtat cggggaagcg tggaagtacg cagctcgagc gcggcgctgg caagcattcc
     5281 tgtggacttg gaggttggat cggttgcgct tccttacgcg gcggtcgcaa ccatggtgta
     5341 tgtggaaccc g
//
LOCUS       DAOSOR010000418         5350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15442_length_5350_cov_3.905382, whole genome shotgun sequence.
ACCESSION   DAOSOR010000418 DAOSOR010000000
VERSION     DAOSOR010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15442_length_5350_cov_3.905382"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="PK710_18600"
     CDS             <1..879
                     /locus_tag="PK710_18600"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOT11794.1"
                     /translation="DIGPGDEVITPAFTFIATAETVALLGAKPVFVDVDPCSYNLDPA
                     RLEEAITPRTKAIIAVSLYGQCADMDAINAIAARHGIPVIEDGAQSFGASYKGRKSGN
                     LSTIACTSFFPSKPLGCYGDGGAIFTSDETLAQLMRQIARHGQQSRYHHVRVGINSRL
                     DTLQAAVLLPKLSIFEEELALRQQVAGRYAAWLASCPVTLPVVAPHNTSAWAQYTLQV
                     SDRRKVCNYLKEKGIPTAVHYPMPLNQQPAVADAKVSLPVGDALAQRVLSLPMHPYLG
                     EDEQQRVARAVMEAAG"
     gene            1058..2944
                     /locus_tag="PK710_18605"
     CDS             1058..2944
                     /locus_tag="PK710_18605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOT11795.1"
                     /translation="MRTVFVGGGRGCRAVLELVIQRRLATLSLQILGVVDLDPDAPAM
                     VLARENGWRTFTDLAEAIRLPDLELIIEVTGIDIVRDEIGRRAPAGVRVMDHTMARVF
                     WDLDEVAQNLRGQLDEKTKLEAEIQQDRRNLQDILDSLPDAVMVVDEQGRIERVNRRF
                     ETLTSLHHGQIQGQYCAAVCCPHEDCSRGVVVPDCPRIQVLASGEPLTVIRQHACIGA
                     HNGDREAYYEVTANPIGSPSGKRRVVITSREVTEQILLTRETKEAAHRFDQIMATVRG
                     IITIKGLDGRYQLANPSASRFFGIPTEAFLGKTARQLFSPAIASVMERNDAAIVRSGG
                     HRSDEEVLVLGGVEKILISERILLRDYKNEIVAICNVSRDVTDSRRLQHELLLTEKHA
                     AVGKLAAGVAHEINNPLTGILTFAEELRDDAEPDSTAREDLDFVVRETLRCRQIVRDL
                     LDFSRQTKPDRQRLRPESVLRQALKLVEKQASFHDIVFRVHVEPNTPEVLADANQLQQ
                     AILNLIINARDAMGGKGAIDCWVGVDEAGMVVLDVKDEGVGIPEEHWARVFEPFFTTK
                     GAQGNGLGLAAVRSIVDQHAGRITVQSTMGKGTVFRVVLPAAGEGRRGSSTEEARKHE
                     GSYS"
     gene            2941..3732
                     /locus_tag="PK710_18610"
     CDS             2941..3732
                     /locus_tag="PK710_18610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT11796.1"
                     /translation="MTQNHDAANRRVLVIDDEDVVHASMRKVLSRLGFGVDGVLSARE
                     GLARLQTDSFDLIITDLMMPQMNGIELLQELRARGNTTPVLMVTGYPTIRTAVQAMRL
                     GAVDYVAKPFTRKELLSPVKRALRIEDQAIHESEGVVHPQSLVPGMTVFLPHHSWARY
                     EQEGTFAVGVEESFLKATGAILAITPPQEIELIEQGFVGLVLSNEEGDAHGVAMPLSG
                     QVVAVNEQAFHDATSLGPETWLLRVLPSALAEELPLLVVKRDSPM"
     gene            complement(3838..4797)
                     /locus_tag="PK710_18615"
     CDS             complement(3838..4797)
                     /locus_tag="PK710_18615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11797.1"
                     /translation="MIFEDQNELKARIEQVTPRRAAKTVVVHTDTTAYMGIEGGHVLR
                     LQGNDYFVLGDAQEGRFGIDDQPKFWVKYAVDLTTAEHKIIKLVFHEQFSTNVGMVRV
                     RCIRSPQKESRVLDLVSGHDRFMQGKTVVDPAGNPVRIVDFIRGKSLYAVLEASDMPH
                     ERYWNEVLPGIMQQLLTCMEAMAWLHSMGEHHGDIRNDHILIERETGRFTWIDFDYEV
                     NFSDYDVWSMGNVLTYVVGQGMHTFSQVQKSPASYPYAQPLQEEDALVLYKHRIANLR
                     KLFPYIPIELNEILMRFSLGTTEPYEDLDSQVRDLRSIFGRLC"
     gene            complement(4794..4985)
                     /locus_tag="PK710_18620"
     CDS             complement(4794..4985)
                     /locus_tag="PK710_18620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11798.1"
                     /translation="MNSTRRVIDFSTESVMSLRENKAFENDRAPPASLVSALKFLPNP
                     PMLRQAEVEPVEPAQTDRQ"
     gene            5045..>5350
                     /locus_tag="PK710_18625"
     CDS             5045..>5350
                     /locus_tag="PK710_18625"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOT11799.1"
                     /translation="MNRQKIANAVLTPADWVLDRLLQTIRQREVFDRISSAAYNTFGG
                     MMRTQFEWLNELAIEGAENVPASGGLVMASNHQSWLDVQVLTASCPRRVHFVAKMEFK
                     "
BASE COUNT         1112 a         1453 c         1612 g         1173 t
ORIGIN      
        1 gatatcggcc ccggcgacga ggtcatcact cccgctttca ctttcatcgc caccgccgaa
       61 accgtggccc tgctcggtgc caaacccgtc ttcgtcgacg tcgacccttg ctcctataac
      121 ctcgaccccg ctcggctcga agaggccatc accccacgga ccaaagccat catcgctgtc
      181 agcctctacg gccaatgcgc ggacatggac gccatcaacg cgatcgccgc gcggcatggc
      241 atcccggtga tcgaagatgg agcccagagc tttggtgcca gctacaaggg acgaaaaagc
      301 ggtaacctca gcaccatcgc ttgcacgagc tttttcccga gcaagcctct cggctgttat
      361 ggcgatggcg gcgccatctt cacgagtgac gaaacgctgg cccagctcat gcgccagatc
      421 gcccgtcacg gacaacagag ccgttatcac cacgtgcgcg tgggtatcaa cagccgtttg
      481 gatacccttc aagccgcggt acttttaccc aagctatcga ttttcgaaga agaattggcg
      541 ctacgacaac aagtggccgg gcgttatgct gcgtggttgg cgtcttgccc cgtaaccctt
      601 cccgtcgtcg cgccccacaa taccagcgcc tgggcgcagt acaccctgca ggtatccgat
      661 cgtcggaaag tgtgtaacta tttgaaagaa aaaggaattc ctactgccgt gcattatccc
      721 atgccgttga atcaacaacc ggccgtggcg gatgcgaagg tcagtctgcc ggtgggagac
      781 gccctggcgc aacgagtgct gagcctgccg atgcatccgt acttgggcga ggatgagcag
      841 cagcgggtgg cgcgggcggt gatggaagcg gcgggttgaa tcccccagcg gcctcggccc
      901 tatcgaagca attttcgaag tcgtatcgcc atggtcgcga gcaggtggca acaagccatg
      961 ttttcgccct gactcatgcg ccacgagttc cgcggctccc atccgcggcc ttcgtgttac
     1021 ccttgtctaa gccccctaac aacacggagt ccagacgatg agaacggtat tcgtcggtgg
     1081 cggacgaggc tgtcgcgctg tgctggaact cgtgatccag cgccggctgg ctacactcag
     1141 cctgcaaatc cttggcgttg tcgacctgga ccccgatgcg ccggccatgg ttttggctcg
     1201 cgaaaatgga tggcgaacct ttaccgatct ggccgaagcc atccgattgc ccgacctcga
     1261 attgatcatc gaggttactg gcatcgatat cgtgcgcgac gagatcgggc gtcgggctcc
     1321 cgccggtgtg cgcgtaatgg atcacaccat ggctcgcgtg ttttgggatc tcgacgaagt
     1381 ggcccagaac cttcgcggtc aactcgatga gaaaaccaag ctggaagcag aaatccagca
     1441 ggatcgtcgc aacttgcagg acattctcga ttctttgccg gatgccgtca tggtggtcga
     1501 tgagcagggc cgtatcgagc gcgtcaatcg tcgtttcgaa acgttgacct cgcttcatca
     1561 cgggcaaatc caggggcagt attgtgcggc ggtttgttgt ccgcacgagg attgttctcg
     1621 cggcgtggtg gtgccggatt gtccgcgaat tcaggtactt gccagcggtg aaccgctgac
     1681 tgtcattcgc cagcatgcat gcattggcgc gcataacggc gatcgggagg cgtattacga
     1741 ggtgaccgcg aatcccatcg gttcgccttc gggaaagcga cgggtggtca tcacctcccg
     1801 agaggtcacc gagcagatct tgctcacgcg ggaaacgaag gaagcggctc atcgtttcga
     1861 tcaaatcatg gcgaccgtgc gaggcatcat caccatcaag gggttggatg gccggtatca
     1921 attggcgaat ccgagtgcct ctcgtttttt tggcattccc accgaagcat ttttgggcaa
     1981 aacggcgcga cagctttttt ctccggccat tgcgagtgtc atggaacgca acgatgcggc
     2041 catcgtgcga agtggggggc atcgcagtga tgaggaagtg ttggttcttg ggggtgtgga
     2101 gaaaatcctg atttcggagc ggattttgct gcgcgattac aaaaacgaga tcgtggcgat
     2161 ctgcaatgtg tcgcgggacg ttaccgattc gcgacgcttg cagcacgagc tattgctgac
     2221 ggaaaaacat gcggccgtgg gcaagcttgc ggcgggtgta gcccatgaaa tcaacaatcc
     2281 tctgacgggg attctcacgt tcgcggagga attgcgcgac gatgcggagc ctgattccac
     2341 cgcgcgggaa gacctggatt tcgtggttcg cgagacgctg cgttgtcggc aaatcgtacg
     2401 ggatttgctc gatttttctc gtcaaaccaa acccgatcga cagcgtcttc gtcctgaatc
     2461 cgtcttgcgc caagcgctca agctcgtgga aaaacaagcg tcttttcacg acatcgtctt
     2521 tcgtgtgcat gtcgagccca acacgccaga agtgctcgcg gatgcgaacc agcttcaaca
     2581 agcgattctc aacctcatca tcaacgcgcg cgatgcgatg ggaggaaaag gggccatcga
     2641 ctgctgggta ggggtcgatg aagcaggaat ggtggtattg gatgtcaaag acgagggtgt
     2701 gggcatcccc gaagagcatt gggcacgggt attcgagccg ttttttacca ccaaaggagc
     2761 ccagggaaac ggcttgggcc ttgcagccgt cagaagcatc gtcgatcaac acgccggacg
     2821 catcaccgtg caatccacga tgggaaaagg tactgtattt cgtgtcgtcc ttcccgctgc
     2881 cggggaggga agacggggtt cttccacgga agaagccagg aaacacgagg ggagctattc
     2941 atgacccaaa atcacgacgc tgccaaccgc agggttttgg tcatcgatga cgaggacgtc
     3001 gtccatgcga gcatgaggaa ggtcttgtct cgtctgggct ttggggtgga cggagtctta
     3061 tcggctcggg aaggtcttgc gcggctccaa accgactcgt tcgacttgat catcacggac
     3121 ctgatgatgc cgcagatgaa cggcatcgag ttgcttcaag agctgcgtgc gcggggcaat
     3181 acgacccctg ttctaatggt gacgggctat ccgaccatac gcactgccgt ccaggccatg
     3241 cgactgggtg cggtcgatta cgtggccaag cctttcacgc gaaaagaact attgagtccg
     3301 gtcaaacggg cgttgcgcat cgaggatcaa gccattcacg agagcgaagg cgtggtgcat
     3361 cctcaatctc tcgtgcccgg aatgaccgtt tttcttccgc accactcctg ggcccgttac
     3421 gaacaagaag gtaccttcgc ggtgggtgtg gaagagagct ttctgaaagc cacgggggcc
     3481 attttggcca tcaccccacc gcaggaaatc gagttgatcg agcagggatt cgtggggctg
     3541 gtgctatcga atgaagaggg cgatgcccac ggcgtggcga tgccgctgag cggacaagtc
     3601 gtggcggtca acgaacaggc gtttcatgat gcgacaagcc tggggcccga gacgtggctc
     3661 ctgcgggtct tgccttcggc gttggcggaa gaattgccct tgctggtcgt caaacgcgac
     3721 agcccaatgt gattcgttgc agagcgtccg agccccagcc cgaattcgga acatgacgca
     3781 taactcgttt gccccatgct gatggccatc tgtccctctc ggttcgtgag aatcacttca
     3841 gcacagacga ccgaagatcg atcgcaagtc gcgtacctgg ctatcgagat cctcgtaggg
     3901 ctcggtggtg ccaagggaaa agcgcattag tatctcgttg agttcgatgg ggatataagg
     3961 gaagagcttg cgcaaattgg caattcgatg cttgtacaag acgagggcgt cttcttcttg
     4021 cagcggctgg gcgtaaggat aggaagctgg gcttttttgc acttgtgaaa acgtatgcat
     4081 gccttgcccc accacatacg taagcacatt gcccatgctc cacacatcgt agtcggagaa
     4141 gttcacctca taatcgaagt cgatccatgt gaagcgtccg gtttcgcgtt cgatgaggat
     4201 atggtcgttg cgaatatcgc cgtgatgttc gcccatgcta tgcagccaag ccatggcttc
     4261 catgcatgtg agcaactgct gcatgatgcc tggcagcact tcgttccaat aacgttcatg
     4321 gggcatatcg gaagcttcga ggaccgcgta taggcttttt cctcggatga agtcgacgat
     4381 tcggacggga tttcctgcgg ggtcgacgac ggttttgcct tgcatgaatc ggtcgtggcc
     4441 ggacacgagg tcgagcactc gcgattcttt ttgggggctt cggatgcagc gaacgcgcac
     4501 catgccgacg ttggtggaaa actgttcgtg gaagacgagt ttgatgattt tgtgctcggc
     4561 ggtggtgagg tccacggcgt atttgaccca aaatttcggc tgatcatcga taccgaagcg
     4621 tccttcctgg gcatcgccga gcacgaagta atcgttgcct tgcaggcgaa ggacatgacc
     4681 gccttcgatt cccatatagg ccgtggtatc cgtatgtacg accacggtct tggcggctcg
     4741 tcgcggcgtg acttgttcga tacgggcctt gagttcgttc tggtcctcga agatcattgc
     4801 ctatccgttt gcgcgggttc gacgggctct acctcggctt gcctcagcat gggggggttg
     4861 ggcaagaact tcaacgcgga tacgagcgag gcgggcggcg ccctatcgtt ctcgaatgcc
     4921 ttattttcac gaaggctcat cacgctttcg gtggaaaaat cgatgacgcg tcgggtcgag
     4981 ttcatgctat ggataacgcg ccgcgttgat tgcggcaatc tgcccccatt tcgaggttta
     5041 aggaatgaat cgacagaaga ttgcgaacgc cgtcttgact ccagccgatt gggtactcga
     5101 ccgcctgctt cagacgattc ggcagcgcga ggtattcgac cgtatttcca gtgctgcgta
     5161 caacacgttc ggcgggatga tgcgcacgca gtttgagtgg ctcaatgaat tggccatcga
     5221 gggagccgaa aacgttccgg cgagcggtgg attggtcatg gcatcgaacc accagagctg
     5281 gctggatgtt caagtattga cggcttcttg cccgcgtcgt gttcattttg tagcgaagat
     5341 ggagttcaag
//
LOCUS       DAOSOR010000419         5348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15451_length_5348_cov_3.894767, whole genome shotgun sequence.
ACCESSION   DAOSOR010000419 DAOSOR010000000
VERSION     DAOSOR010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15451_length_5348_cov_3.894767"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1373
                     /locus_tag="PK710_18630"
     CDS             141..1373
                     /locus_tag="PK710_18630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11800.1"
                     /translation="MKNGLLWVALLGIGLMNCGTDDEGTSDTKPKAPDAGGVDGHPGD
                     DASAHGGSGGAAGAGGASAGAAGHSTAGSGGTGSSGAGGSAGGAAGTGGSSGQGAAAG
                     ANGDYLASQYPGDKGIENDPAVVWVENFEEGSVDALVARYNDAKKNGLTLDAQSHPNS
                     IGKASGKFTANGSGPQAVDLYKNFGAGYDELFVRYYVKYGSGAQWHHTGVWLGGYAPP
                     SNWPSPQAGLKPEGNDRFHISFETMGSSPNPRLDFYNYWMKMHSWKDNPTGSDAYYGN
                     TIVHQKDRIAHDNQWMCVEIHLKLNTNPASSQGAELGLWIDDQSVIQFNDSSPQGCWI
                     KDKFCPQTGPVADDPSCTDYPNLCKKPYVPLDLQFRSTTDLRINAFWPQNYITEGGAG
                     TVWYDDMVLARSRIGCIP"
     gene            complement(1414..4158)
                     /locus_tag="PK710_18635"
     CDS             complement(1414..4158)
                     /locus_tag="PK710_18635"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOT11801.1"
                     /translation="MPPSKALPENATTAWHSLSRDEALERLDSSDEGLSSAEAARRLD
                     IHGRNILAEVSGDGPLIILWRQINNPLIWVLIGSGILAVALGKVTDGSVVLAVVVLNS
                     IIGFYQEYRAGKAIEALRGMVPEFANALRDGTVAVVPVVDLVPGDVVMLASGDKVPAD
                     VRLLGVKNLRVEEAALTGESVPVEKSLDPVVEDAALGDRTSLAFKGTLVTYGTAKALV
                     VVTGTKTELGRISKMLEEATELVTPLTRALESLGKIITVAILVVSGVILAFGVYRALQ
                     VGVALGDALKETLIFAIALAVGAIPEGLPAIVTIALAIGVQRMAARRAIIRKLPAVET
                     LGSTAVICSDKTGTLTRNEMTVHALWTLEDGSVSVEGVGYDPKGTFCTSDNCHEVTPT
                     MKRLLENAALCNDGTLEKKETGWQITGDPTEGALVVAAEKAGIDVRVLRKRMARQDAI
                     PFESENQFMATLHADEWTSRVVVKGAPEVIARRCALVNGAKLDLTPHVEALAAQGMRV
                     LAVAEKPWKKESLELTDQDVSSELTFCGLVGMIDPPRPEAIEAVRACHTAGIQVKMIT
                     GDHEGTARAIGAQLGLSKEVKAMQGSALPKLEPAKLREVVENTSVFARVAPEHKLLIV
                     KTLQETGHIVAMTGDGVNDAPALKQANIGVAMGITGTSVSKEAADIVLTDDNFATIVA
                     AVEEGRRVYDNLVKSLAFVLPTNLGLAFILMYAVLLLPFDPISKALLLPVQPTQLLWI
                     NLVAAVALALPLAFEAKEVNVMNRPPRDPKEPIMSKLVVRRTILASVLMTIGAVGLFQ
                     FVYGQSIASEGGEHTLAEAQTMAVTTVIFFQVFYLMNCRSLRDSVFKIGFFSNPIVFW
                     GIGITLILQAAFIYAPPLQAVFGTAPLDAKELGLSFLAGVVTLPIITLEKWISARSRG
                     "
     gene            complement(4236..>5348)
                     /locus_tag="PK710_18640"
     CDS             complement(4236..>5348)
                     /locus_tag="PK710_18640"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT11802.1"
                     /translation="LPKHGTKAELIRYQTQDRGKLIDATALLTYPSSKGTYPILLILH
                     GTAGFTDKCAPTAGMVGADWGGFEEELGVLAALYASFGYIVVFPDYIGLKSFGSPTGF
                     LHPYLVAEPTAIASLDSVRATKKRLASSSTVPGDVVVIGGSQGGHAAAFVNRYLPHYA
                     PELTIKGSVWDVPPTDLTEQSRAALSVSWENATKNAIAFFATSNEWYASAPGGLSEIL
                     LPPLDTEVPASMASECKGTKFDDPTLEKVFTPQVLAAAKEPDFGTFEPWACYMKESSL
                     SSTSVPRLDDIPSMFLLGEKDALVNNTVERAAFQELCAQGLKLVYLECAGATHTKPLV
                     WAFDQTLDFLEDRLNGKPLPADTCLLRAAETCTSQP"
BASE COUNT         1214 a         1654 c         1428 g         1052 t
ORIGIN      
        1 cgaccaccca cgcgaaagcg cagttcattt ttttcgttcc acgaagcccc aggagttgag
       61 gcagatggct agataggcca tgctcacctt tgggttttcg agaatgtctg cacttgcctt
      121 ggtccacgcc ggaggaaatc atgaaaaatg gcctgctttg ggtagcattg ttgggcatcg
      181 gtttgatgaa ttgcggcacg gatgacgagg ggacgtcgga cacgaaaccc aaagcccccg
      241 atgccggagg agtcgacggc cacccagggg acgatgcgtc agcgcacggc ggtagcggcg
      301 gtgccgcagg ggcaggggga gcttccgcgg gtgcagccgg ccattccacc gcgggttccg
      361 gcggcacggg gagttcaggt gcgggcggat cggctggcgg cgcggccgga acgggtggat
      421 cgagcgggca gggcgctgcc gcaggcgcca acggcgatta cctcgcttcc caataccccg
      481 gtgacaaagg catcgaaaac gatcccgctg tcgtctgggt cgagaacttc gaggaaggtt
      541 ctgtcgatgc tctcgtcgca cgctacaacg acgccaagaa aaatggcttg accctcgatg
      601 cccaatcgca tcccaacagc attggcaaag caagcggtaa gttcaccgcc aatggctccg
      661 gaccccaggc cgtcgaccta tataaaaact tcggagcggg atacgacgaa ctattcgtcc
      721 ggtattacgt caaatacggc agtggtgccc agtggcacca cacgggcgtg tggctgggag
      781 ggtatgcacc accctccaat tggcccagcc cccaagccgg tctcaaaccg gaagggaacg
      841 accgcttcca catctccttc gaaaccatgg gcagcagccc caatccgcgt ctggacttct
      901 ataactactg gatgaaaatg cactcgtgga aagacaatcc caccggctcc gatgcctatt
      961 acggaaatac aatcgtccac cagaaagacc ggatcgccca cgataaccaa tggatgtgcg
     1021 tggaaattca cctgaaactc aataccaacc cggcttcgag tcagggagcg gaactcgggt
     1081 tgtggatcga cgatcaatcc gtcattcagt tcaacgattc atcgccccag ggatgctgga
     1141 tcaaagataa gttctgtccg caaacaggcc cggtcgccga tgatccgagc tgcacggact
     1201 atccgaatct atgcaaaaaa ccatacgttc ccctggattt gcagttccgc tccacgacgg
     1261 atttgcgcat caatgccttc tggccgcaga actatatcac cgaaggaggc gcgggaaccg
     1321 tgtggtacga tgatatggtt cttgctcgct cgcgcatcgg ttgcatcccc taagccgacg
     1381 atatttcagt ctcgccgtcg catcaccatc cgttcatcca cgagaccttg cactgatcca
     1441 cttttcgagg gtgatgatgg gaagcgtgac cacccccgca aggaacgata atcctagttc
     1501 tttcgcatcc aacggcgccg tcccaaatac cgcctgcaac gggggcgcgt aaataaacgc
     1561 cgcctgaaga atcaacgtga tcccaatgcc ccaaaaaacg atgggattgg agaaaaaacc
     1621 aatcttgaac acggagtcac gcaacgaacg acagttcatc aagtagaata cttgaaagaa
     1681 aatcaccgtg gtcaccgcca tcgtttgcgc ttccgccagc gtatgctccc ctccctcgga
     1741 agcgatcgat tgcccataca cgaattgaaa gagacccacc gcaccgatcg tcatcaacac
     1801 cgaggctagg atcgttctgc gaacgactag cttgctcatg ataggctctt tcgggtcgcg
     1861 cggaggacga ttcatcacgt tgacttcctt ggcttcgaac gccaagggca acgcgagcgc
     1921 cacggccgcc acgagattga tccagagcaa ttgtgtgggc tgcaccggaa gcaacaacgc
     1981 tttggagatt ggatcgaaag gcaagagcaa caccgcgtac atgaggatga acgccagccc
     2041 caggttggtg ggaagcacga aagccaagct cttgaccagg ttatcgtaga cccttcgacc
     2101 ttcctcgacc gccgccacga tcgtggcgaa gttgtcatcc gtgagcacga tgtccgcggc
     2161 ttctttggac acggacgtgc ccgtaatccc catcgccacg cctatattgg cttgttttaa
     2221 cgccggcgca tcgttgaccc catcaccagt catcgcaacg atatggccgg tttcctggag
     2281 cgtcttgacg atgagcagct tgtgttcggg ggccacgcgg gcaaaaacag acgtattttc
     2341 aactacttcg cgtagttttg caggctcgag cttgggcagg gcgctgcctt gcattgcttt
     2401 gacttctttc gaaagaccga gctgggcacc gatggctcgc gccgtgcctt catggtcccc
     2461 cgtgatcatt tttacctgga ttcccgctgt atgacaggct cgaacggctt cgatggcctc
     2521 cggtcgcggc ggatcgatca tgcccaccaa tccgcaaaac gtaagctcgc tggagacatc
     2581 ttggtccgtc aattctaagc tctctttttt ccagggcttt tccgccaccg caagcacacg
     2641 cattccttgc gccgccaacg cctccacgtg cggagtcaaa tccagtttcg ccccgttcac
     2701 caacgcacat cgtcgcgcaa tcacctccgg agcacccttg accacgaccc tcgacgtcca
     2761 ttcatccgca tgcaacgtag ccatgaattg gttctcggat tcgaacggaa tcgcatcctg
     2821 acgtgccatg cgtttgcgca atacccgaac gtcgatccca gccttttctg ccgcaaccac
     2881 gagcgcccct tccgtaggat cgcccgtgat ctgccaaccg gtttcctttt tctccaatgt
     2941 gccgtcgttg cacagcgccg cgttttccaa caaccgcttc atcgtggggg tgacttcatg
     3001 acaattgtcg ctcgtgcaaa atgttccctt cggatcgtag cccacccctt ctaccgatac
     3061 gctgccgtct tccaacgtcc acagggcgtg aaccgtcatc tcattgcgcg tcaacgtgcc
     3121 ggttttgtcc gagcaaatga ccgcggtgga gccaagagtc tcaaccgcgg gaagcttacg
     3181 gatgatcgcg cgacgcgctg ccatgcgctg cacgccaatc gcaagcgcaa tggtcacgat
     3241 ggccggcagt ccctccggaa tggctcccac cgcaagagca atcgcaaaaa tgagcgtctc
     3301 cttcaacgca tcgccgagcg caacccccac ttgtagtgcg cgatatacgc cgaacgcgag
     3361 gatgactccg gatacgacca aaatggctac cgtaattatt tttccaaggg attcgagcgc
     3421 gcgagtcaag ggcgtgacca actccgtggc ctcctcgagc atcttcgaaa tacgacccaa
     3481 ttccgtcttg gtgcccgtca ccaccaccaa agccttggcc gtcccatacg tcaccaacgt
     3541 ccctttgaaa gcaagagacg ttcggtctcc cagcgcggca tcctccacca ccggatcgag
     3601 cgacttttcc acagggaccg actctcccgt caacgccgcc tcttccacgc gaaggttctt
     3661 gaccccaagc aaacgaacat ccgctggaac cttgtctccc gacgcaagca tcaccacgtc
     3721 gcccggcacc agatccacca cgggaaccac ggcaacggtg ccgtcgcgaa gggcgttggc
     3781 aaactccggc accatgcccc gcaacgcttc gatggcttta ccggcgcgat actcttgata
     3841 aaaaccaatg atgctattga gcaccacgac cgccagcacg acagaaccat cggtgacctt
     3901 gcccaacgcg acagccagga tcccggaacc gatcaatacc cagatgagcg ggttgttgat
     3961 ctgacgccac agaatgatca aaggaccgtc accggatacc tcggcgagga tgttgcggcc
     4021 atgaatgtcg agtcgtcgcg ccgcttccgc ggaagacagg ccctcatcgg atgaatcaag
     4081 tcgctcgaga gcttcatcgc gcgacagaga atgccaggcg gtggtcgcgt tctcaggtag
     4141 ggctttcgaa ggaggcatgg actctccaag ggagtgcggt caagcgacac cacgctatcc
     4201 gcacgtcagc cagttgcaac atcaaggtgg aaaaatcaag gttggctagt acacgtttcc
     4261 gccgcgcgaa gcaaacaggt gtccgcgggc agcggcttgc cattaagccg atcttcgaga
     4321 aaatcaaggg tttgatcgaa tgcccacacc aacggcttgg tgtgcgttgc ccccgcacac
     4381 tccagatata caagcttcag accttgcgca caaagctcct ggaacgccgc ccgctccacc
     4441 gtgttgttca ccaaagcatc cttttcacca agcaaaaaca tgctgggaat gtcatccaga
     4501 cgcggtaccg acgtcgatga cagcgagctt tctttcatgt aacaggccca cggttcgaag
     4561 gtcccgaaat ccggctcctt cgcggctgca agcacttgcg gcgtaaacac tttctcgagc
     4621 gtcggatcgt cgaatttcgt gcctttgcat tccgaggcca tcgacgccgg aacctcggta
     4681 tcgagcggtg gcagcaggat ctcactcagc cccccaggcg cgctcgcata ccactcgttg
     4741 gaagtcgcaa aaaaggcgat cgcattcttc gtggcgtttt cccacgatac cgatagcgcg
     4801 gcccgcgact gctccgtcaa gtccgtaggg ggaacgtccc ataccgagcc cttgatggtc
     4861 aactcgggcg cataatgagg aagatagcgg ttgacgaacg ctgcggcatg acctccttga
     4921 gaaccgccga tcaccaccac atcacccggg accgtggacg acgatgccag acgttttttg
     4981 gtggctcgca cggaatcgag ggaagcgatg gccgtcggct cggccacgag gtagggatgc
     5041 aaaaaaccgg tggggcttcc gaagcttttc agaccaatat agtcaggaaa aacaacgata
     5101 tagccaaacg acgcgtaaag ggctgccagc acacccaact cttcctcgaa gcctccccag
     5161 tcggctccta ccatgccggc ggtgggcgcg cacttatccg taaatcctgc ggtgccgtgc
     5221 aggatcaaca ggatgggata cgttcctttc gacgaagggt aggtcagcaa cgcggtggcg
     5281 tcgatgagct ttcctcggtc ttgtgtctga taacgaatca actcggcttt cgtgccatgc
     5341 ttgggcag
//
LOCUS       DAOSOR010000420         5314 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15592_length_5314_cov_4.828675, whole genome shotgun sequence.
ACCESSION   DAOSOR010000420 DAOSOR010000000
VERSION     DAOSOR010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15592_length_5314_cov_4.828675"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PK710_18645"
     CDS             complement(<1..536)
                     /locus_tag="PK710_18645"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT11803.1"
                     /translation="MRRRTFTPLRDEVTELSLGTWGLSGEGYGAVSAAEADAVLDRAV
                     QLGINLYETSDAYNRGEMLTRLGQRLASTPNAYVAVRVGIDRSDESQPKKNFDPAYLR
                     EAVAKAGEKLKRSRIDIVLLHNPSVSVVERGEATGTLEDLRRVGEIGAWGVSAGSREV
                     AVAALESGTHVLSIAYNL"
     gene            complement(562..2751)
                     /locus_tag="PK710_18650"
     CDS             complement(562..2751)
                     /locus_tag="PK710_18650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11804.1"
                     /translation="MASRKTLASIVLAIHRSTCNGALVRWTWMTRMDRMVGWLRPVIA
                     YLPALLVSGMLAYASLSAIVQHVGHPAVPLDDAFIHFQFARNLAAGRFFQYMPGEGYV
                     AGATSFLWPLLLAPFYLIGFRGLSILWVAWFFGFLALAGVFLEVFRLTEKLTGRAVAL
                     GASAMCAFFGAFTWFAASGMETMALAWVLLRGARLAAEWFENPETRSQKSRNHLILLG
                     IIAPGIRPEGAMVSLFAAIALLISPFGTGKRQRALGALPLLGLLFDPVIHLMLTGSTT
                     SNTTAVKWLPANPYYGFRELWDTVGYNLRVFFQVLLDGQQWSAVFLPRGTLPFALLAL
                     GSIPLMGWRSQRPWRAGLVFFIALGMMIPCTYHTFLWNRLRYLWPFAPAWFIGMACFT
                     KLAGQMLSLVRPRWGLIAPMLAGIVAGVLAGNMKWTVDDLVKSAAAIDLQQVELGRWA
                     KENLPAEAIIGVNDTGAIAYLSERRTFDVIGLTTQGEGRYWVAGAGSRFEHYERLYAS
                     EPHRFPTHFIVYPHWMACEPVLGKRLHEASVYDQTILGGTTMVVYEARVDLLGSGARP
                     ILIPYSGEIIDELDVADLESEQAHAYEIAHGGKDEANKVHTDFFFEDRPGGIDSGQDE
                     NTSINDRIWADGGRFERVADRFVAHLRPGQPAKAIARWVGAHEGEVELEIKSGSTPLA
                     TIRLGAYRAQENEFIIPAQVASEHTPIEVRALHGGTFGSLHYWFVAQ"
     gene            2787..3431
                     /locus_tag="PK710_18655"
     CDS             2787..3431
                     /locus_tag="PK710_18655"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOT11805.1"
                     /translation="MNAVPAHKKPHPYADAPTLPAVGSLDIFSLTRSRLPLEIEIGPG
                     RGAFIVERAAEAAQAAVVGLEIRYKWAKIVDDRLARRGFGDRARVFAADARFVLPRLQ
                     PDASVSCFFIHFPDPWWKKRQQKRLVVTPPFVREMVRLLEPGGFVFVQTDVLERGEQY
                     ETLLSTMEGLQPDGDEPGSPVVKEVFWEAKSNRERRVLAAGLTPSRLLFRKPRG"
     gene            3503..3862
                     /locus_tag="PK710_18660"
     CDS             3503..3862
                     /locus_tag="PK710_18660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11806.1"
                     /translation="MAEPPAVPPQDQDHRAPVFHLEWLVAAMIGMGIAMGVAGAVAYL
                     ESSRFPPAPPAESGAREWGTARTRPPKTSAPEVSDEVEDSVADETSEPVIDVEISHSG
                     RAPADSQAIGSDSEPGP"
     gene            4365..>5314
                     /locus_tag="PK710_18665"
     CDS             4365..>5314
                     /locus_tag="PK710_18665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11807.1"
                     /translation="MNGAKRLSYSVFCALFLSIAGCNDDAQSSAASSGVGGSGGVSGS
                     GGASGSGGVSGSGGDGMSGSSGASGSGGASGSGGASGSSGASGSGGTSGSGGTSGSGG
                     ASGSSGASGSSGASGSGGASGSGGTSGSGGTSGSGGTSGSGGTSGSGGAGGFSTAPGR
                     PLPEPIYGVTSEGVSSVSALSEALERHGRDVTVRIVFQEGTNPKDYTTRVNTVRQHAY
                     VMGEILDSTGLAGMTTQAYRARAKVFVEQFGDSIDIWEIGNELNGEWVGTPTTINAKV
                     LAAYEVVHQEYAHLNLRSAVTLNALVKHHPTNNLRTAASGL"
BASE COUNT         1172 a         1507 c         1618 g         1017 t
ORIGIN      
        1 aagagattgt acgcaatgga aagcacgtgg gtcccagatt cgagggctgc gacagccact
       61 tcccggcttc cggcactgac gccccaagcg ccgatttctc ctacgcgacg caggtcttca
      121 agagtgcctg tcgcttctcc ccgctcgacg accgacaccg atggattgtg gagcaatacg
      181 atatcgatgc gcgaccgttt cagtttttcc cctgccttgg ccaccgcttc ccgaagatag
      241 gcgggatcga agtttttttt cggctggctt tcatcggatc gatcgatgcc cacacgaacc
      301 gccacataag cgtttggcgt cgacgccagt cgctgcccca ggcgcgtgag catctcccct
      361 cggttgtagg cgtccgaggt ttcataaaga ttgatgccca attgcacggc ccgatccagc
      421 acggcatcgg cctccgccgc ggatacggcg ccatatcctt cgccggacaa accccaggtt
      481 cccaagctca gttcggtgac ctcgtcgcgc aacggagtga aggtacgtcg tctcacaaca
      541 gcagatctaa gggacgcgac ttcattgcgc aacaaaccaa taatgtagcg agccgaaggt
      601 gcccccgtgc aacgcgcgca cttcgatggg ggtatgctcc gaagccacct gcgcgggaat
      661 gatgaactcg ttttcttgtg cccggtacgc accaagacga atcgttgcca gcggcgttga
      721 acctgacttg atttcgagtt caacttcacc ctcgtgggca cctacccatc gggcgatggc
      781 tttcgctggc tgccccggtc gtagatgggc gacgaaacgg tcggccacgc gctcgaatct
      841 tcccccatca gcccatatcc tatcgtttat acttgtgttt tcgtcttgtc cggaatcgat
      901 accccccggt cgatcctcga aaaaaaagtc cgtgtgaacc ttattcgctt catcctttcc
      961 cccatgggcg atctcgtagg catgcgcctg ttccgattcg aggtccgcca catcgagttc
     1021 atcgatgatt tcaccggagt agggaatcag aatcggtcga gcacccgagc cgagaagatc
     1081 gacacgggct tcgtaaacca ccatcgtcgt gccgccgaga atcgtctgat cgtagacgct
     1141 cgcttcgtgc aaccgcttgc ccaataccgg ctcacaggcc atccaatggg gataaacgat
     1201 gaaatgggtg ggaaatcgat ggggttccga tgcgtagagc cgttcgtaat gctcgaaccg
     1261 ggaacccgct cccgcgaccc agtatcgtcc ttctccctgg gtggtgagcc caatgacatc
     1321 gaaggtccgt cgttccgata ggtaggcgat ggctcccgtg tcattgaccc cgatgatcgc
     1381 ctcggcgggc aggttttcct tggcccaacg ccctagttcc acttgttgca agtcgatggc
     1441 ggcagcgctc ttgaccaaat catcgacggt ccatttcata ttgcccgcaa gcacaccggc
     1501 cacgatgccc gcgagcatcg gcgcgatgag cccccatcgc ggccgcacga gcgaaagcat
     1561 ctgccccgct agcttggtga aacaggccat tccgatgaac catgccggag caaaaggcca
     1621 tagataacga agacgattcc aaagaaacgt gtgatacgtg caaggaatca tcatcccaag
     1681 ggcgatgaaa aacaccaatc cggctcgcca cggacgctgg gagcgccatc ccatcaaggg
     1741 aatggagccc agggccagca atgcaaaagg cagggttccc cggggcaaaa acaccgcgct
     1801 ccactgctgg ccgtccaaca gaacctgaaa aaaaacgcgt aggttgtacc ctacggtgtc
     1861 ccacaattct cgaaaaccat aatacgggtt ggcgggaagc cacttgaccg ccgtggtgtt
     1921 cgaggtcgtg gaacccgtga gcatcaggtg gatgacgggg tcgaacagca ggccgagcag
     1981 aggtaatgcg ccaagggccc gttgccgttt gcctgtgccg aaaggggaga tgagcaatgc
     2041 gatcgctgca aatagcgaaa ccatcgcccc ctcagggcgt atccccggag ctataatacc
     2101 taataaaatc aagtgattgc gagatttctg ggagcgcgtt tccgggtttt cgaaccattc
     2161 ggctgccagc ctggcaccgc gcagcagtac ccatgcgaga gccatggttt ccatgccact
     2221 ggccgcaaac caggtgaatg caccgaaaaa cgcgcacatt gccgacgcgc caagggctac
     2281 cgcccgcccc gtcagttttt ccgtgagccg aaacacctcc aaaaaaacgc ctgccaacgc
     2341 caaaaaaccg aagaaccagg caacccaaag gatcgacagt ccacgaaatc cgatgagata
     2401 aaacggtgcg agcagcagag gccacaaaaa actcgtggca cccgccacat aaccctcgcc
     2461 aggcatgtac tggaaaaaac gcccggcggc gagattgcgg gcaaactgaa aatgaatgaa
     2521 cgcatcgtcc agcggtactg cgggatgtcc cacgtgctgc acgatcgccg acagcgatgc
     2581 gtacgcgagc attcccgaca ccagaagagc cgggagatag gcaatcacag gccgaagcca
     2641 gccaaccatc cgatccatcc gtgtcatcca ggtccaacgc accagcgcgc cattacatgt
     2701 cgatcgatgg atcgcaagga cgatcgaagc cagtgtcttg cgggaagcca tggccggaat
     2761 ggccgcgaag cggtaaggaa gggagcatga acgccgtccc cgcgcacaaa aagcctcatc
     2821 cttatgcgga cgctccgacg cttcccgcgg tgggttctct tgatattttc agtctcactc
     2881 gctcgagatt gcccttggaa atcgagattg gccccggtcg aggggctttc attgtcgaac
     2941 gcgcggcgga agccgcccag gccgctgtcg ttggcctgga gatccgctat aaatgggcga
     3001 aaatcgtcga tgatagattg gctcgacgcg gcttcgggga tcgagcaagg gtttttgcag
     3061 cggatgctcg attcgtcctt cctcgtcttc agcccgatgc cagtgtgtct tgttttttta
     3121 tccactttcc cgatccgtgg tggaaaaaac ggcagcaaaa acgcctggta gttaccccac
     3181 cttttgttcg tgaaatggtc cggctgctcg aaccgggggg attcgtcttc gtgcaaaccg
     3241 atgtgttgga gcgtggcgag caatacgaaa cgctgttgtc gaccatggag gggttgcagc
     3301 ccgatgggga tgaacccggt tccccggtcg tgaaggaagt gttttgggaa gcgaaaagca
     3361 atcgggagcg aagggtgctt gccgccgggt tgaccccatc gcggcttctt tttcgaaaac
     3421 ctcgcggatg acagcgcgtg cctgcggtac gggaatggtt tgtgccgttg acaatcccct
     3481 tgcgatagca tgatgtgtgg ccatggccga gccccccgcc gtcccgccac aagaccagga
     3541 ccatcgagcg cccgttttcc atctggaatg gttggtggcc gcgatgatcg gaatgggaat
     3601 cgccatgggc gtggcggggg cggtggccta tctcgaatcg tctcgttttc cgcctgcgcc
     3661 accggcggaa tcgggggctc gtgaatgggg caccgcccga acgcgcccgc ccaaaacatc
     3721 cgcccctgag gtttcggatg aagtggagga tagcgtggcg gatgagacga gcgagccggt
     3781 gattgatgtc gaaatcagtc attccgggcg tgcgcccgcg gattctcaag ccataggaag
     3841 cgactccgag ccgggcccat aggtggcgtc gagcgatcgc ctcgtggggg aaatatcgtt
     3901 tggggatctt ccaattcgat gctgagcagg gggctggctc taacagatcc cctcattttt
     3961 ttctgaattg gaaaggggta cgagctgagg atttttggcg cggggaaaaa tgaggggatg
     4021 gctcagggca acaatcggta ccgtaggggc gggtttcaaa cccgcccgcg cgtgcgaatg
     4081 gctcagggga tgaccgtgac cgtttgtcgt gggccgtgac cgtgaccgtg accgtggccg
     4141 tgaccgtggc cgggcgatgg taggtagttg tatctagcgg tcgtcgggag aaggaaaggc
     4201 gcgaggaaaa atgaggggat ggctcagagg ttggttggcc ccgggggagg gagagataac
     4261 cccggattaa ataattcagt aataaattca caatgttatg gcgctaatgg acgactgctt
     4321 cttcgggcct tggccgtgtc attttatggt acattccatt catcatgaac ggggcaaaga
     4381 gactgtcgta ttcggtgttc tgtgcgctgt ttctatcgat tgccggttgc aatgacgatg
     4441 cgcaatccag cgccgcttcg tcgggagttg gcggcagcgg aggcgtgagc ggaagcggcg
     4501 gtgccagtgg tagcggaggc gtgagcggaa gcggcggcga tggaatgagc ggcagcagtg
     4561 gagccagcgg cagcggtggg gcaagcggca gcggcggggc aagcggcagc agtggagcaa
     4621 gcggcagcgg tggaacaagc ggcagcggtg gaacaagcgg cagcggtgga gcaagcggca
     4681 gcagtggagc aagcggcagc agtggagcca gcggtagcgg tggggccagc ggcagcggtg
     4741 gaacaagtgg tagtggtgga acaagcggca gcggtggaac aagcggcagc ggtggaacaa
     4801 gcggcagcgg tggggccggt gggttctcca ccgcgccagg tcgacctttg cccgagccga
     4861 tctacggagt cacctccgaa ggcgtcagct ccgtcagcgc cctttcggaa gccttggaac
     4921 gtcacggacg cgacgttacc gtgcgcatcg tatttcaaga aggtacgaac cccaaagact
     4981 acaccacgcg cgtcaatacc gtgcgtcagc atgcctatgt catgggagaa atcctcgact
     5041 ccacgggact cgcggggatg accacccagg cttatcgcgc ccgagcgaag gtttttgtgg
     5101 agcaatttgg tgattcgata gatatttggg aaattggaaa cgaactcaac ggcgaatggg
     5161 ttggaactcc cacgaccatc aacgccaagg tgctcgctgc ctatgaggtc gttcaccagg
     5221 aatatgctca cctgaacttg cgcagcgccg ttaccttaaa tgcgcttgtc aagcaccatc
     5281 cgaccaacaa tctccggacg gccgcaagcg gcct
//
LOCUS       DAOSOR010000421         5287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15700_length_5287_cov_4.611430, whole genome shotgun sequence.
ACCESSION   DAOSOR010000421 DAOSOR010000000
VERSION     DAOSOR010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15700_length_5287_cov_4.611430"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..493)
                     /gene="cutA"
                     /locus_tag="PK710_18670"
     CDS             complement(128..493)
                     /gene="cutA"
                     /locus_tag="PK710_18670"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOT11808.1"
                     /translation="MVWKDDVRDVVVLYCTVPNLEVGSSVARVLVQEGLVACVNLIPG
                     LQSIYRWKGEICEDSEVMLMMKTVRSRTEEIAKRVKSLHPYETPELVSLPVRGGLEGY
                     LAWVREQACVGCLRSAQRD"
     gene            584..2428
                     /locus_tag="PK710_18675"
     CDS             584..2428
                     /locus_tag="PK710_18675"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT11809.1"
                     /translation="MIDCPACHQTNPLGATFCIYCGDVLPDSTPDSAPASADGTVPGV
                     PMSGALHPNLTPGSASLTTGHIVDGKYSIDSILGEGGMGVVYLATDIHTNVPVVVKAI
                     RSEYAHREEFRNRILAEGRALARIDHQNVVRLNAVVVEPDGLFLVMQYIDGESLDKTI
                     SRYVAQQTPMPVPLVLKIFHQVLDGVGAAHAEGVIHRDLKPANILIRRRDAVAKVTDF
                     GIAKAEEDIQAGRGQTKGIIGSLLYMAPEQIKGLRDLDNRVDIYGLGILLYEMLVGRV
                     PFDAPSEFEIMKLHLEAEIPLISRMRSDVPAYLDTAIHRACAKNREDRFTTTAEMAAA
                     LQADSTLRGPSHDDSASAYPVGLASPMGRPGPAFDPPARTVTAESAEFEQPKGRSPLF
                     WIVLSLSLFLLGAGAVFGAAYGGLFSPKPPKRRTSPTTSAPWVPPTPSVSQMPSAAVT
                     PPVAPPAATPVAPVVNPLETLVGSWKSDTGRVYDAVMSGRALEFRIHDARGMEQDGYV
                     TGEERFVLEAIEGQPTVFSVKDKIRPALPADLSYDLTRSRSSCQEVRTELNGKPLRAR
                     FDGARLTVEVVKLAPDRSMFLREGSKVNGCVRLREAKASMLQSTLVRP"
     gene            complement(2429..3199)
                     /locus_tag="PK710_18680"
     CDS             complement(2429..3199)
                     /locus_tag="PK710_18680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11810.1"
                     /translation="MTQAEADAYCGAGARFDGYGCVRIVCPAGQELDMATGSCLAKQQ
                     VDKVAESMGVQVGEGQKLGCPPGLILVVEDARNASCVPPESTCSRDETWNGQACVKAY
                     QCAPGWIFDSVSGQCIAVSTSESDYTIDLQAWTSASYGASGGPGSFAFCSGFNKKPRT
                     FGVGPGGRAQVLIDVQVQAPGRSVMQAQVSTNGRMQSHGGPVTPQGAAEIQRVAQEIL
                     ASLLVQGGKTATESARTSVGCTIVNAAAPAPVPESGGL"
     gene            3563..4624
                     /locus_tag="PK710_18685"
     CDS             3563..4624
                     /locus_tag="PK710_18685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11811.1"
                     /translation="MMRGHHDESIRLALVGTTESQSCASIHPKIRWFIRASFALTLAL
                     GLASLWFARTSLIVATCVVAAAFLAALTNYVRKTRRFSKPHASLTMSRSCIALERQGA
                     PSEEVFSFLPQFGFVLLATADRSVWVVALTNRWQTFCVGSIVGPSDKWRFRKLISRAV
                     TTCRSELTRAVQSSKSISMHVEAQSLIRLIEWAKTLDAASLGRILLTDGLGNDVVLEQ
                     DQVLTSRGIFRLDQPLDWRGLVFHQGNDSLAVEYQGTWVRQGRSEMVLVSLRGDDVRQ
                     SIHDVAAVSSFRADGALKQDARLAASAASDPPAIEQRVAVDRLFMLPFRRALDTAPSE
                     IPKSIPEVFRGDATRPDER"
     gene            complement(4671..>5287)
                     /locus_tag="PK710_18690"
     CDS             complement(4671..>5287)
                     /locus_tag="PK710_18690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11812.1"
                     /translation="NESLSAEQPATTSAMQDADETRLEASSEVANHTPEMAERRDSIH
                     EVPVDDEVRPAVESASASIPTAQQDIVEIEMGEAVALEELVPSHAQRMKRNKLMSLTL
                     LDGTTLALPMRRRGRRKESLVDSLAEEKSAPAALDVGRDAGEDAVPIRDIIAALRAAA
                     RGAAPSEALGNQAKSEAILAVVLSLLIRKGLITEDELLEELNKI"
BASE COUNT         1066 a         1653 c         1410 g         1158 t
ORIGIN      
        1 aacctgtgca gaataggctc ccatcgcctt ccgatcgaac cgccgcacga tgacatctcc
       61 cacccatcat cgatacaatc cgtaaacgga tattacttgc gacgacccga tcatcggctc
      121 gttgggttca gtcccgttgt gccgagcgaa ggcaccccac acaagcttgt tcgcgaaccc
      181 acgccaagta accttccaat ccacctcgca ccggcaggga caccaactcc ggcgtttcat
      241 acggatgaag agatttcacc cgtttggcaa tttcctccgt gcgactccga accgtcttca
      301 tcatcaacat gacctcggag tcctcacaga tctccccttt ccagcgatag atggactgaa
      361 ggcctggaat gaggttcacg cacgccacca agccttcctg caccaacacc cgtgccacgg
      421 aagaccccac ctccaggttg ggtaccgtgc aatacaatac caccacatcc cgaacgtcgt
      481 ctttccacac catggctcct ccgacgtctc cacaaacgtt ttcaagcctc ccgcatcgtg
      541 ccacactctg ctcacttcgt cgcctgttcg gatcgaactc gtcatgatcg attgccctgc
      601 ttgccatcaa accaacccgt taggtgccac cttctgcatc tattgtgggg atgttttacc
      661 cgactcgacc cccgactcgg ctcccgcctc ggccgacggt accgtgcccg gagtcccgat
      721 gagcggagcc ctgcacccca atctgactcc tggatccgca tccttgacca ccggccatat
      781 cgtcgatgga aaatattcca tcgattcgat cctaggcgaa gggggaatgg gggtcgtcta
      841 cctcgctacc gatatccaca ccaatgtccc cgtcgtcgtc aaagccatcc gatccgaata
      901 cgctcaccga gaagaattcc gtaatcgaat cttggccgag ggacgggcct tggcgagaat
      961 cgatcaccaa aacgtcgtgc ggctcaacgc tgtcgtcgtc gaacctgatg gcctttttct
     1021 cgtcatgcaa tacatcgacg gggaatcctt ggataaaacg atcagtcgtt atgtcgcgca
     1081 acaaacaccg atgcccgtgc cgctggtgct caagattttc catcaggtgc tcgatggtgt
     1141 gggagcggct cacgcggaag gcgtcattca tcgcgacctg aaacccgcga atatcttgat
     1201 tcggcgtcgt gatgcggttg cgaaggtgac ggacttcggt attgccaagg ccgaggagga
     1261 cattcaagcg gggcgagggc aaaccaaggg aatcattggg tccttgctat acatggcgcc
     1321 ggaacagatc aaagggctgc gggacctgga taatcgagtg gatatctatg ggctcggtat
     1381 tttgctgtac gagatgctcg tgggaagagt ccccttcgat gcgcccagcg aattcgagat
     1441 catgaagctc catttggaag ctgaaatccc cttgatttca cgcatgcgct ccgacgtccc
     1501 cgcttatctc gatacggcca ttcacagagc atgcgccaag aatcgagagg atcgtttcac
     1561 cacgaccgcg gagatggcgg cggctttgca ggcggattcg acgcttcgtg ggccttccca
     1621 cgatgattcc gcttcggcgt atcccgtggg gctggcctct cccatgggtc gtcccggtcc
     1681 agcgttcgat cctcccgctc gcacggtgac cgcggagagc gccgagttcg aacagcccaa
     1741 aggccgttcg cctctgtttt ggatcgtctt gtcgttgagc ctttttctgc tcggtgcagg
     1801 agcggttttc ggcgctgcgt acggaggcct gttctccccc aaacctccca aacgccgcac
     1861 ctcccccacc acttccgccc cttgggttcc ccccacaccg agcgtatctc aaatgccttc
     1921 cgcagcggtc acgccgcctg tggctccgcc tgctgcaacg cctgtcgcac ctgtcgtcaa
     1981 ccccctcgaa acgctggtgg gatcatggaa aagcgacaca ggacgagtct acgacgcggt
     2041 gatgagtggc cgcgcgttgg agtttcgaat ccatgatgct cgtgggatgg aacaagatgg
     2101 gtatgtgacg ggcgaggaac gattcgtcct cgaagccatc gaagggcagc caaccgtgtt
     2161 ttccgtgaag gataaaatac gccccgctct tcccgcggac ttgtcctacg acttgactcg
     2221 ctcgcgttcc tcttgccagg aagtgcgcac ggaactcaat ggtaagccat tgcgcgcgcg
     2281 tttcgatggc gctcgtctta ctgtcgaggt ggtcaagctg gcaccagacc gcagcatgtt
     2341 tttacgggaa ggttcgaaag taaacggctg tgttcgtcta cgggaagcca aagcctcgat
     2401 gctgcaaagc accctcgttc gcccttgatc acaatcctcc ggattccgga acgggggccg
     2461 gagccgccgc gttgacgatg gtgcaaccca cggaggtacg agcggattct gtcgccgttt
     2521 tgccgccttg caccaacaag cttgccagta tctcttgggc tacccgctga atctccgcag
     2581 ccccctgcgg cgtgacaggc cccccatggc tctgcattcg accattcgtc gaaacttgcg
     2641 cttgcatgac cgaccgacct ggcgcttgaa cctgaacgtc gatcaacact tgtgcccggc
     2701 cgcccggacc taccccaaac gttctcggtt ttttgttgaa acccgagcaa aaagcaaagc
     2761 tcccgggacc tccagaagcg ccatagctcg cggacgtcca ggcttgtaga tcgatggtgt
     2821 aatccgattc gctcgtgctc accgcgatgc attgacccga aaccgaatcg aaaatccaac
     2881 ccggcgcgca ttggtaagct ttgacgcacg cttggccatt ccatgtctcg tcgcgtgagc
     2941 aggtgctttc tggcggcaca cacgatgcgt tgcgcgcatc ttcaaccacg aggatcagac
     3001 ccggtgggca gccgagtttc tgcccttccc ccacttgaac ccccatactt tcagccactt
     3061 tatccacctg ctgcttggca aggcaactgc ccgtggccat atcgagttcc tgccccgctg
     3121 gacatacgat tcggacgcaa ccatacccat cgaaccgcgc ccccgcgccg caatacgcat
     3181 cggcttccgc ctgggtcatg caggaactat tgagagatac tctccccgga ttacacacac
     3241 atgtatcgcg ctgtgtgtcg aatgtgctgc cactaggaca agtatcccag gagcatcggc
     3301 agttcgatga gttgcccttg cagactttca ccagacacaa gcccgatgga cagccagagg
     3361 tgcctagggg gaccagacca aggaccacga gcctacccac cacagttcca agcttcgaaa
     3421 aaagccagcg catcatctta ctccgtggtc cgaacgctag caccctaacc gccatccggg
     3481 tagccatgcc gctgtgccta tcgtcacgac ccacgagacg caaggcaagc ttgcgctaca
     3541 atgaaaatca acggcgttga ccatgatgcg tggacatcac gacgaatcga ttcgtttggc
     3601 actcgtcgga acgaccgagt cgcaaagttg tgcttccata catcctaaaa ttcgctggtt
     3661 catcagagcc tcgttcgccc ttaccctggc gttggggctc gcttcgttgt ggtttgctcg
     3721 cacgtcgctc atcgttgcga cgtgtgtggt ggcagccgct tttctcgctg ccctgacaaa
     3781 ctacgttcga aagacccggc ggttctcgaa gccgcacgct tccttgacca tgagccgttc
     3841 ttgtatcgcc ctggaacgtc aaggtgcgcc ctcggaggag gtgttttcat tccttcccca
     3901 attcggattc gtattgcttg cgactgccga tcgctccgtt tgggtggtag cgttgacgaa
     3961 tcgatggcag acattttgtg tcggatcgat cgtgggccca tcagataagt ggcgattccg
     4021 caagctgatt tctcgggctg tcaccacttg ccgaagcgaa ctcacccgtg ccgtgcaatc
     4081 ctccaagagc atctcgatgc acgtggaggc acaatcattg attcgcctca tcgaatgggc
     4141 gaagactctc gatgccgcat cgctcgggcg gatcctgctg accgatgggc tgggcaatga
     4201 cgtcgtgctc gagcaagatc aggtgttgac gtcccggggc atttttcgat tggatcaacc
     4261 gttggactgg cgcggtctcg tttttcatca aggcaacgat agcctcgcgg tagagtatca
     4321 aggaacttgg gttcgtcagg gccgcagcga aatggtgttg gtgtctttgc ggggagacga
     4381 tgttcgccaa tcgattcacg atgttgcggc cgtctccagc ttccgtgcgg acggtgcgtt
     4441 gaaacaggat gcaagacttg ctgcgagcgc tgcatccgat ccccccgcca ttgagcaacg
     4501 agttgccgtg gatcgactgt tcatgctgcc atttcgacgc gccctcgaca cggcgccttc
     4561 tgaaataccg aagtcgattc ccgaagtttt cagaggcgac gctaccagac cggatgaacg
     4621 atgactcgat agtgcttgac ctgcagcccc cgggcgcatg gtccgatgca ctagattttg
     4681 ttcaactctt cgagcaattc atcctccgtg atcaaacctt ttcgaatcaa caaagataaa
     4741 acaacagcca gtatggcttc cgatttggct tgattcccaa gagcttcgga cggggcggca
     4801 ccgcgagcgg ccgcgcgcaa tgcggcaatg atgtcccgta ttggcacggc gtcctcgcct
     4861 gcatcccgcc caacgtccag cgctgccggc gcggacttct cctccgccaa cgagtccaca
     4921 aggctttcct tacgtcggcc tcgacgacgc atgggaagag cgagtgtcgt cccatccaag
     4981 agcgtcaaac tcatcagctt gtttcgcttc atacgctggg catgggacgg caccaattcc
     5041 tccagggcca cggcttcgcc catttcgatt tcgacgatgt cctgttgtgc ggtgggaatc
     5101 gacgcactcg cactttccac cgcaggacgt acttcatcgt ctaccggtac ttcgtgaatg
     5161 gaatcacgac gttcggccat ctctggggtg tggttcgcga cctcggagga cgcttccagg
     5221 cgtgtttcgt cggcatcttg catggccgag gtggtcgcgg gctgctcggc ggagagcgac
     5281 tcgttcg
//
LOCUS       DAOSOR010000422         5274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15747_length_5274_cov_4.341828, whole genome shotgun sequence.
ACCESSION   DAOSOR010000422 DAOSOR010000000
VERSION     DAOSOR010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15747_length_5274_cov_4.341828"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PK710_18695"
     CDS             <1..550
                     /locus_tag="PK710_18695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11813.1"
                     /translation="VQGAIALVFPEGQPAWLHGPLATILGQTRDLEEGPSPIDIYGGN
                     MVFRRDLFDELAAFREDLGKGAAGYSEDIELTRRLRQRQETIAYAPTARIFHVIGPDR
                     STARFLRDNSFDKGYSDGLIAKGSHLRLLGKSALKAYVHGWLALVMLPNRHRSVVEQT
                     RAWDHAGRVLGLWKVRLNKAKT"
     gene            550..1476
                     /locus_tag="PK710_18700"
     CDS             550..1476
                     /locus_tag="PK710_18700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT11814.1"
                     /translation="MKPTICVLVTSYGRPRYLRQCLLSLFSQTRLPDEIVLVTRVGDT
                     PTEQAVSELLQADHGVVLRHGRAKQAGVLPANRVGLGLARSDIVAFLDDDAAARPDWV
                     RRIQQWFSDNPRLGALGGRDIVHTEHGVKDEVTQRVGQISWYGRIVGNHEKVFLGVTK
                     ADHLKGVNMSFRRELIGEFDENIIGNAHHYEMDMCFAVKKRGFEIVFDGGLVVDHYQE
                     APRHLTGNQAGADALREYFIHRNRVYVMMKNLGNARRGAFLLYTFFLDAGSDLGEVLL
                     RKPGIDSRIWWSMMRGKAAGLRAFLETRAQKL"
     gene            complement(1464..2558)
                     /locus_tag="PK710_18705"
     CDS             complement(1464..2558)
                     /locus_tag="PK710_18705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11815.1"
                     /translation="MRAFYSLMFVTMGSLVLHTATAWAQPADSRGQPSGEPSSDEASP
                     PSLPSSSIPAVEAAPVEAASTTVTEIPQVPPCGAGAIKGVDPIDAETAVWLVCQEIHK
                     YSSRPASLSPADPPAVYRVGLMKLGEKVFLTVHVEAPVGSVRDSRQISLASIEELPIA
                     TPRVVKALFAATAVSDTQTISTLVGEENREYKKRHGEYLFGGGLVGSMFLGMGARIVP
                     ALDLRVGYETEQLGIGLGTRISLDNSRYDEDSTELFTFQVGARYFLSNENTSLFFGGG
                     LAYASMEARKDGEYYEGGGLGAYGEVGVELLRLYSSRLTFDLQVFAPTFPLKTGEYFS
                     GWDPETGVYTPQPEKSMYAVPVLVGVSYSF"
     gene            2858..3496
                     /locus_tag="PK710_18710"
     CDS             2858..3496
                     /locus_tag="PK710_18710"
                     /inference="COORDINATES: protein motif:HMM:NF015946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11816.1"
                     /translation="MSDPFTPIIHDSATWQRRFVIVGTRTKASSAFHLHALGDSGGRM
                     DIMVRCMRAALLVADGIRRDTVLDLLLLAGADSPLAVRLSGESIRFLRPDERRNAILL
                     QEALATWHPGKEMTHLAPGIEARPGGLEEWLCRNHGENLFYLDSGGPDIRSVGLVDGG
                     VTFVVGDDRGLTQEQREILVRHKVQPLGLGPVSVHTEDAITLVHNELDRRWL"
     gene            3642..>5274
                     /locus_tag="PK710_18715"
     CDS             3642..>5274
                     /locus_tag="PK710_18715"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HOT11817.1"
                     /translation="MRTVVRNLSIVSCLSLATGWIACSGSVTEKNEEPSQSTSQLMRR
                     ATSCEDLEAALKADARARLERLMMEQDHDTGYAGAGGSMGMDAGSFAGSGGAMTGGSG
                     GAMAGGMGGGTAGGGGGNESGPEGGGPNQYTGTNTQVANVDEADFVKTDGLRIYQLRD
                     QMLHISKAWPVTEASAQSSIQIEGTPLEMFLVGDAGSPDQKLVVYSTVDGYDLFQQAG
                     LEIPTFSGTWDPSGWTSPDPNTSPTELTKISVISVASASPALVHEVYFEGRYTSSRRN
                     GLHVRTVMDVPGRLPELRVYPTEYEQQKAEMLVVEELLKSGKSVEDIPADELDMMVRE
                     ALGKVMVKENELIIEGIRAQDWLPHVFSKSAGQIVASSVACQDFYVPKPGTSQYGITH
                     VEPLDLGDLGASPAGLGIVGVAQTMYENANAMVLATSTMEGSWSDPFYSTYVHRFELG
                     EGALPTYSGSGVIPGYIKGQFSIDEKNGFIRIVATAGRQWGSEPLTTYLSTLEKVDGE
                     LKVAGQTEGFGYMESVYVVRFVGDTAYVVTFRQTDP"
BASE COUNT         1158 a         1383 c         1552 g         1181 t
ORIGIN      
        1 tgtacaagga gcgattgcct tggtcttccc cgagggacag cccgcgtggc ttcatggacc
       61 tttggcaaca atccttggac agacccgcga tctcgaagaa ggcccgtccc ccatcgatat
      121 atatggcgga aacatggtat ttcgacgaga tctcttcgat gaactcgctg ccttccgcga
      181 agatctcggc aaaggagctg ccggatactc cgaggatatc gagttgaccc gtcgcttgcg
      241 ccaacgacag gaaaccattg cctacgcacc cacggcgcga atatttcacg tcattggtcc
      301 cgaccgctcc acggcccgtt tccttcgaga caattcgttc gataaaggct attccgatgg
      361 tctcattgcc aaaggatctc acttgcgctt gctgggaaaa tccgcactca aagcgtatgt
      421 ccacgggtgg ttggcgctcg tgatgctccc aaaccgtcac cgtagtgtcg tggaacaaac
      481 ccgggcctgg gaccacgcgg gacgggtatt ggggctgtgg aaggttcgac tgaacaaggc
      541 gaaaacctaa tgaaaccgac gatttgcgta ttggttacga gctatgggcg cccacgctat
      601 cttcggcaat gcctgctgtc gctgttttcg caaacgagat tgccggatga aatcgtgttg
      661 gtgacccggg tgggtgacac accaaccgag caagctgtct cggagcttct tcaggcggat
      721 catggtgttg tgctgcgtca cggacgagcc aaacaagctg gggtattgcc tgccaaccgt
      781 gtgggattgg gattggcccg gagcgatatc gtggcgtttt tggatgatga tgcggctgcc
      841 cgacccgatt gggtgcgccg tatccagcaa tggttttcgg ataatccgcg ccttggagcc
      901 ttgggtgggc gagatatcgt tcacaccgag cacggcgtga aagatgaagt cacgcagcgt
      961 gtcgggcaaa tttcttggta cggtagaatt gtgggaaacc acgaaaaagt atttttgggc
     1021 gtgaccaaag ccgaccacct caaaggcgtc aatatgagtt ttcgccggga gctgattggc
     1081 gaattcgatg agaacatcat tggcaacgcg catcattacg aaatggatat gtgttttgcc
     1141 gtgaagaaac ggggattcga gattgtattc gatggggggt tggtggtgga tcattaccag
     1201 gaggcgccgc gtcatttaac gggtaatcaa gcgggggccg atgctttgcg cgagtatttc
     1261 atccatcgta atcgcgtgta tgtcatgatg aaaaatctgg gaaacgcgcg gcggggcgct
     1321 tttcttctat atacattttt tctcgatgcg gggtcggacc tgggggaggt gttgctgcgc
     1381 aaaccgggaa tcgattctcg gatctggtgg tcgatgatgc gcggtaaagc tgcgggttta
     1441 cgtgcttttt tggagactcg ggctcagaag ctataagaca cgcccacaag gacgggtacg
     1501 gcatacatgc ttttttcggg ttgtggtgtg tagactccgg tttctggatc ccatccgctg
     1561 aagtattcac cggttttcaa cgggaaagtg ggcgcaaaga cctgcagatc gaatgtgagc
     1621 cggctcgaat acaaccgcaa cagctccaca cccacctcgc cgtaagcccc caaccctcca
     1681 ccttcgtaat actcaccgtc ttttcttgct tccatcgaag catacgccag ccctccgcca
     1741 aaaaatagag aagtgttttc attcgacagg aagtatcggg ctcccacctg gaaagtaaaa
     1801 agttccgtgg agtcctcgtc atagcggcta ttgtccagac tgatacgagt accgagcccg
     1861 ataccgagtt gttccgtttc atagcctacg cgtaaatcca gcgcgggaac gatacgagcc
     1921 cccattccca ggaacatcga accaaccagg cctcccccaa aaaggtattc cccatggcgt
     1981 tttttatact cgcggttctc ttcgcccacc agggtggata tcgtttgcgt atcggagacc
     2041 gctgttgccg caaatagggc tttcacgacc ctgggcgtgg cgatcggcag ttcttcgatg
     2101 cttgccaggg agatctgccg actatcacgt accgagccga caggagcctc cacatggacg
     2161 gtaagaaaga ctttttcgcc caacttcatc aatccgactc gataaacggc tggcggatcc
     2221 gccggggaca aggaggcggg acgactggaa tacttgtgaa tctcctgaca tacgagccat
     2281 acggcggtct cggcgtcaat ggggtcgact cccttgatgg cccctgcgcc gcacggcggg
     2341 acttggggta tttcggtcac cgtggtcgag gccgcctcca cgggggccgc ttcaacggcg
     2401 ggaatgctgg aggatggcaa cgaggggggc gacgcttcgt cgctcgaggg ttccccactc
     2461 ggctgtccgc ggctgtcggc aggttgcgcc caagcggtcg cggtgtgcag caccaacgag
     2521 cccatcgtga cgaacataag agaataaaaa gcgcgcatga ttgcctcctt ctgataaaat
     2581 gcaagggcca atcaaatacc gtgcccaatg ccgcatccaa cgtagtatcg cggaattcat
     2641 gcttaaaatc tggtttaggt cgcttttatg ttgggagttt ttcgccacac tgttcccatc
     2701 caccacagtg tcgttcgtgc tttttttcca cctatccccc tacggacggc aggagcttgg
     2761 ataagaacgg ggcgacagcc accgggattc cctgccgcga cgcaccccgg cgcaattcat
     2821 tgggtgtcgc cagggtggcg cgtataatct acggctcatg tcggatccgt tcacccccat
     2881 catccatgat tccgccacct ggcagcgccg tttcgtgatc gtcggtaccc gcaccaaagc
     2941 atcctcggct tttcatcttc atgcgctcgg cgactcgggc gggcggatgg atatcatggt
     3001 tcgatgtatg cgcgcggcgc ttttggtggc ggatggaatt cgacgggaca ccgtgctcga
     3061 tttgctgctt ctggccggtg cggattctcc ccttgccgtg cgcttgtcag gagaaagcat
     3121 ccgttttttg aggcccgacg aacgtcgcaa tgccatattg ctccaagaag ccctcgccac
     3181 ctggcatccg ggaaaggaaa tgacacatct tgcgccaggc atagaggccc gacccggagg
     3241 gctggaagaa tggttatgcc ggaatcacgg cgaaaacctg ttttatctgg attccggggg
     3301 cccggatatc cgctcggtgg ggcttgtcga tgggggagta acgttcgtgg tgggcgatga
     3361 ccggggattg acacaagagc agcgggagat cttggttcga cataaggttc agccgttagg
     3421 tctcggtccc gtatcggttc acaccgagga tgccatcacg ctcgtccaca acgaattgga
     3481 tcgccgttgg ctttgagtac accatcttat gtgccatctt gtgccattct gacaaggaga
     3541 tcgggaatac tgcagttcaa taagtaataa atgtaaaaga aacatatagt tatgaacatg
     3601 gcacacagca tgcttatagc gagcccggag gattcaacac catgagaacc gtcgttcgta
     3661 acctttccat cgtgagttgt ctgtctttgg ctaccgggtg gattgcttgc tccgggtccg
     3721 taaccgagaa aaatgaggag ccctctcagt ccacgagcca attgatgcgt cgtgccacaa
     3781 gctgtgagga tcttgaagcg gctttgaaag ccgatgcgcg agcgcggctc gagcgtctca
     3841 tgatggagca agaccacgat actgggtatg cgggtgccgg tggctccatg ggcatggacg
     3901 ccggctcttt cgccggttca ggcggtgcga tgacgggagg ttcgggcggt gccatggccg
     3961 gaggtatggg tggtggcaca gcagggggag gggggggaaa tgaaagcggt ccggaggggg
     4021 gaggaccgaa ccaatatacc ggcactaata cccaagtagc caatgtcgac gaagcggatt
     4081 tcgtgaagac cgatggcctt cgcatctacc agcttcgtga ccagatgctc catatctcga
     4141 aggcttggcc tgtgacggaa gcctcggctc aatcttccat ccagatcgaa ggtactcccc
     4201 tcgaaatgtt ccttgtcggt gatgccggct cccccgacca aaaactcgtt gtctactcca
     4261 cggtggatgg ctacgacctc tttcaacaag caggactgga aatcccgaca ttcagtggaa
     4321 catgggaccc aagtggctgg acttctcccg atcccaacac atcacccacc gaacttacaa
     4381 aaatttcagt gatttcagta gcttccgcat cgcccgccct ggtgcatgag gtgtatttcg
     4441 aaggacggta tacgtcgtcg cggcgcaatg gtctacatgt gcgcacggtg atggacgttc
     4501 caggacgttt gcctgaacta cgtgtgtatc cgacggaata cgaacaacaa aaagcggaaa
     4561 tgcttgttgt ggaagagttg ttgaagagcg gcaagagcgt ggaggacatt ccggccgatg
     4621 aattggatat gatggtccgc gaagcgctcg gaaaggtgat ggtaaaggaa aacgaactga
     4681 tcatcgaggg tattcgggcg caggattggt tgcctcatgt attcagcaaa tcggcagggc
     4741 agattgtcgc cagctctgtg gcttgccaag atttctacgt ccccaaacct ggtacgagcc
     4801 aatatggcat tacgcatgtg gagcccctcg acttgggtga cctcggggct tcgcccgcgg
     4861 ggctgggcat cgtcggggtc gcacaaacca tgtacgaaaa cgcgaatgcg atggtgttgg
     4921 ccaccagcac catggaagga agttggagcg atcccttcta ttcgacctac gtgcatcgtt
     4981 tcgaattggg cgaaggagcg cttcccacct acagcggctc cggtgtgatt cctgggtata
     5041 tcaaagggca gttttccatt gatgagaaga atgggttcat tcgaatcgtg gctaccgcgg
     5101 ggagacagtg ggggtccgag ccgttgacaa cctacctgtc cacgctcgaa aaagtggatg
     5161 gagaattgaa ggttgccggc caaacagagg gcttcggata tatggagtcg gtctacgtgg
     5221 tacgattcgt gggggatacg gcctacgtgg tgacattccg ccagaccgat cccc
//
LOCUS       DAOSOR010000423         5270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15767_length_5270_cov_4.287248, whole genome shotgun sequence.
ACCESSION   DAOSOR010000423 DAOSOR010000000
VERSION     DAOSOR010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15767_length_5270_cov_4.287248"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PK710_18720"
     CDS             <1..503
                     /locus_tag="PK710_18720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT11818.1"
                     /translation="IRVCGQVVEPKSVESIRARVGFLFNVPEDQLLFPKVVDDTAFAL
                     LRRGMSAAQAKEKARDVLERLGIGHLVDHPVHVLSHGQKQRVALAGALVTQPPVLLLD
                     EPSAALDPRAKRNLAQLLRDQQAALLVATHDLSFAEAICTRYVLLEGGHIEYDGAEVS
                     EVRRRL"
     gene            605..1423
                     /gene="nagB"
                     /locus_tag="PK710_18725"
     CDS             605..1423
                     /gene="nagB"
                     /locus_tag="PK710_18725"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOT11819.1"
                     /translation="MQILILHTAQQVEALAARMIADRLKAKPNLVLGCATGRTMEGVY
                     DRLVELASREKLDFGRVTTFNLDEYVGLTADDPQSYHYYMAEKLFSRVNLRPGRTHVP
                     NGMARDLAAEAVSYEERIKAAGGIDLQLLGIGNTGHIGFNEPLSSLSSRTRDLALTPM
                     TQEQNAGMFGDDPSKVPTRALTMGVGTILEAREALLIVTGAHKAQILATAVEGPVTSM
                     VSATALQFHANCCILVDEAAAEKLVGREEYRRTFVHEPTWAPYRDFILSSERNA"
     gene            complement(1548..2885)
                     /locus_tag="PK710_18730"
     CDS             complement(1548..2885)
                     /locus_tag="PK710_18730"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOT11820.1"
                     /translation="MRAFYFLMGAAGVCLGTTLSFEAAAQTCNPPRILFVVDASSSML
                     DDITEGGTTVSKWAAAEDAVSAVLSAYGEAAQYGLMMFPGSSGGCATGEINVEIGANK
                     APEILSALSAINWSASRQTPAGQSLMASSKYAGITDPAYNNYVIFLSDGWQYCSIPTG
                     STPVCVSAADCTLMSVDPCPSCNPCQNASSDPNCKGKPADGCFCVRKWPVLGVQALAA
                     AGVSTYVVGFGSQVDAKTLNQAAEAGGTALTGCDPNSDSASCYYQATAPSSLNAALAD
                     IVQQVVVDSCLGPCDIPGERTCTASGWSACDAPDHVECTSACGTKGTQQCVNGTLTPC
                     DAECPDSGAAGAGGAGGTGGASGQGGEAGVGGEGAQGGKGGEGATGGTGAVGGTGGEQ
                     TDAGLDKPKPPAPEDTGEDGGCACHTGRTAPSPIAAAFVALMAALGLRIRRRR"
     gene            complement(2970..4709)
                     /locus_tag="PK710_18735"
     CDS             complement(2970..4709)
                     /locus_tag="PK710_18735"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOT11821.1"
                     /translation="MTVNTLPLKMPRACRPCFALFALGAIACSDPASIDDAQDGDEYP
                     EAVGPIEDYGKADSTGMGAAHKPLPEGASLDGNFRVFYGPHEPMMAVELELLNQVIDA
                     RRADPRTFSNADNPYRIRYAVYNLRAREVVDALGKAKKANVFVQVLVDWRQMGPDYPW
                     NTAYDYLEKKGFTVYRHQKGLTETQRREANVIGVEVKGYMHLKTRLFEHPGNTVLITG
                     SANPGENVAFSDDSLHVVREPRIVDAFARCYDAILFGQRVVNEWEEEAPINALFSGNA
                     TGVSAGTQILRWFEQEQEQILINVFSLRDFTAKDVSDSLVSLLKRKVEQGVPVYVITD
                     RKQSDGVDENMKQVNSDEPTDDRLRQAGVKVYETLNTASPFTAMHHKAAVLGRSRIRV
                     ISGSANWSFSGLGSRTEPAKNLESVLFLDTQRLDDGLTGRRYLANWFRILNVYAHQGV
                     QIDHEAPFSDVFAQFTGTQDWPQESLWFDVRDGDVGDNRIFVAGDVSALGAWGAQHSG
                     LPLVTDEQNYPRWWSEDPVELPLGAPFVWRLWAGASETGKKTWEKTKNRNGLAGPGPF
                     DTPSKVVIDAQWQ"
     gene            complement(4768..>5270)
                     /locus_tag="PK710_18740"
     CDS             complement(4768..>5270)
                     /locus_tag="PK710_18740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11822.1"
                     /translation="LLMGQEFGEPWGLGFRKSAFMPSRFEGTEQFHPQGQSLVQSYGA
                     MNRARLEPANQALLSANHYFLRPRNTPQDPDSRLFAQAKWTAGSVVFVFHNLWNQDVS
                     QSFALPGELTALLGIVAQGSYRMVDILNNDEPGPCTTGSGLAREIPITMKASERMKWL
                     RLEICR"
BASE COUNT         1080 a         1494 c         1541 g         1155 t
ORIGIN      
        1 cgattcgggt ctgcggtcag gtcgtggagc ccaagtcggt ggaatcgatc cgtgcccgcg
       61 ttggattttt gttcaacgtg ccggaagatc agcttctgtt tccgaaggtc gttgacgata
      121 cggcgtttgc tttgcttcga cgaggaatga gtgcggctca ggccaaagaa aaggctcgcg
      181 acgtgctgga acgactgggg atcggtcatc tcgtggacca tccggttcac gtgctgtccc
      241 acggtcaaaa gcagcgcgtg gcgctcgctg gagccttggt cacccaaccg cctgtgctgc
      301 ttctcgacga gccttcggcg gctttggacc ctcgcgcaaa acgaaatctt gcccagcttc
      361 tgcgcgatca gcaagccgcc ctgttggtgg cgacccatga cctttcgttc gcggaagcca
      421 tttgcacgag atacgtgctg ctcgaagggg gccatatcga atacgatggc gcggaggtga
      481 gcgaggtgcg tcgaaggctg taaccatgtg aattaccata ggttttcaaa aaaaatcgtg
      541 aactttcatt ttttgtgtac ggaccacctt ttcttcgcgg gggcacgtac attagattgc
      601 gcccatgcaa atactcatcc ttcatacagc tcagcaggtg gaagccttgg ccgcacgcat
      661 gattgccgat cggttgaaag cgaagcccaa ccttgttttg ggctgcgcca caggccgaac
      721 gatggaaggt gtttacgatc ggctcgtgga attggcttcg cgtgaaaagt tggattttgg
      781 ccgcgtgacc accttcaatc tcgacgaata cgtgggtctt accgccgacg acccgcaatc
      841 ctaccattac tatatggcgg agaagctgtt ttcccgtgtc aatcttcgcc ccggtcgaac
      901 gcatgttccc aacggcatgg cgcgtgacct ggccgcggaa gccgttagtt acgaagaacg
      961 aatcaaggcc gcggggggga ttgacttgca actcctgggg attggaaata caggacatat
     1021 cggtttcaat gaaccgctgt cctcattgtc ttcccgcacc cgggatcttg ccctcacacc
     1081 catgacccag gagcaaaacg cggggatgtt cggcgatgat ccgagcaagg tacccacacg
     1141 cgctctcacc atgggcgtag gcaccatcct cgaggcacga gaagcgctct tgatcgtaac
     1201 aggggcgcat aaagcccaaa ttctcgctac cgccgtcgag ggccccgtca cgtcgatggt
     1261 cagtgcgaca gcgcttcagt tccacgccaa ctgctgcatt ttggtggatg aagccgcggc
     1321 cgaaaagctt gtggggcgag aggagtatcg aaggactttc gtccatgaac ccacctgggc
     1381 tccttatcgc gatttcatcc tgtcatcgga acgcaatgcg tgaaaggtgg gccttcgacc
     1441 gagcgaaatt gcgaaagcga aaccgtggca ggtgcgaccc gaaacgtcag cgtttcgtgg
     1501 caccggcatg cctgtaatta tttgaaataa aacgagaata tggcgattca gcgccgtcgg
     1561 cgaatcctca ggcccagcgc agccatgagc gcgacaaaag ccgcagcgat aggggagggg
     1621 gccgtgcgcc cggtgtgaca ggcacagccg ccgtcctctc ccgtatcttc gggtgctggg
     1681 ggcttgggtt tatccaatcc ggcatccgtt tgttcgccac cggtgcctcc gacggctccc
     1741 gttccgccgg tggcgccttc gccgcccttt ccgccttggg ctccctcacc gcccacaccg
     1801 gcttcgcctc cctgcccgct ggctccaccc gtgcccccgg cacccccggc acccgcggcg
     1861 cccgaatcag ggcattccgc gtcacaaggt gtcaaggttc cattgacgca ctgctgggtt
     1921 cctttggttc cgcaagcgga agtacattcg acgtgatcgg gcgcatcgca ggcggaccag
     1981 ccggaagccg tacacgttcg ctctccaggg atgtcgcaag gccctaggca tgagtcgaca
     2041 accacctgtt gcacgatatc agccagggcg gcgttgaggc tggaaggcgc cgtggcctga
     2101 tagtaacaac tggcggagtc gctgttggga tcgcatccgg tgagtgccgt gcctcccgcc
     2161 tccgctgctt ggttcaaggt tttggcatcg acctgacttc caaaacccac gacgtaggtg
     2221 gagactcccg cggccgccag cgcttgtacg ccgagcacgg gccatttgcg cacgcagaag
     2281 cagccgtcgg caggtttccc cttgcagttg ggatcggaac tcgcgttttg gcacgggttg
     2341 caggaaggac aggggtccac gctcatcagc gtacaatccg cggcagagac gcataccgga
     2401 gtggaaccgg tggggatgga gcaatattgc cacccatcgg ataggaaaat cacgtagttg
     2461 ttgtaagcgg gatccgtgat gccggcgtac ttggaggaag ccatgagcga ctgacccgct
     2521 ggggtttgtc gagaggcgct ccagttgatt gccgacaacg cggataggat ttccggggct
     2581 ttgttggctc cgatttccac gttgatctca ccggtagcac aacctccgga agagccggga
     2641 aacatcatca atccgtattg cgcagcctcg ccataggcgg acaacaccgc gcttacggcg
     2701 tcttccgcgg ctgcccattt ggagacggta gtaccgccct cggtgatatc gtcgagcatg
     2761 gatgaggaag catccacgac gaatagaatg cgaggtgggt tgcatgtttg tgcggctgcc
     2821 tcgaaagaca atgtcgttcc gagacatacg cctgccgctc ccattagaaa atagaaggct
     2881 ctcatgatac gatagaatat cctcaaatcg ctgtgagcgc aaagacgatt ttgtgctcgg
     2941 ttttcagaat ttcggcatcg aatggggtgt tactgccact gcgcatcgat gacgactttg
     3001 cttggcgtat cgaagggccc cggacccgcc aggccgtttc ggtttttggt tttttcccag
     3061 gttttcttgc cggtttccga agctcccgcc cacagacgcc aaacgaaagg tgcgcctagg
     3121 ggaagttcca ctggatcctc cgaccaccac ctcggataat tctgttcgtc agtcacaaga
     3181 ggcaatccac tgtgctgagc cccccatgcc ccaagagcgc tcacgtctcc cgccacaaat
     3241 atccggttgt ctcccacatc accgtctctg acatcgaacc acaaggactc ttggggccaa
     3301 tcctgcgtac cggtgaattg agcgaaaaca tctgaaaatg gggcttcatg gtcgatttgg
     3361 accccttggt gcgcatatac gttgagaatg cgaaaccagt tggcgaggta tcgtcgcccc
     3421 gtcagcccgt cgtcgagccg ttgcgtatcc agaaataaaa cgctttccag attttttgcg
     3481 ggctccgttc gtgaacccaa ccccgaaaat gaccagttcg ccgagcccga aatcacacga
     3541 attcggctgc gtccgagcac cgccgccttg tgatgcatcg ccgtaaacgg cgatgccgtg
     3601 ttgagcgtct catagacttt cacccccgct tgccgcaaac ggtcgtcggt aggctcatca
     3661 ctattgacct gcttcatgtt ctcatcgaca ccgtcggact gctttctatc ggtaatcaca
     3721 tagacaggca cgccctgttc gacttttcgt ttgagcaagg aaacgagcga gtcactcacg
     3781 tcttttgccg taaagtcccg taacgaaaac acgttgatga gaatttgctc ctgctcctgc
     3841 tcgaaccaac gaaggatctg cgtaccggcg gaaacacccg tcgcatttcc cgaaaataac
     3901 gcgttgatgg gcgcctcctc ctcccattcg ttgacgaccc gttgcccgaa aaggatggcg
     3961 tcgtaacagc gcgcaaaggc atcgacaatt ctgggttctc ggacgacatg cagggagtcg
     4021 tcgctgaagg cgacattttc tccaggattt gcgcttcctg tgatcagtac tgtatttcct
     4081 ggatgctcga agagccgtgt tttgaggtgc atatagcctt tgacttcgac accgatgacg
     4141 ttggcttccc ggcgttgggt ttccgtcaaa cctttttggt ggcggtagac agtgaagcct
     4201 tttttctcga ggtagtcata ggcggtattc caggggtagt cggggcccat ctgtcgccaa
     4261 tccaccagta cttgcacgaa tacattggct tttttggctt tccccagggc gtcgacgact
     4321 tcccttgcgc gcagattgta gacggcatag cgaatgcgat aaggattatc ggcgttggag
     4381 aaagtgcggg gatcggcgcg acgagcatcg atgacctggt tgagcaattc gagttcgacc
     4441 gccatcatgg gttcgtgggg gccgtaaaag acacggaagt tgccgtccag ggaggcacct
     4501 tcgggaaggg gtttgtgggc agcgcccatt cccgtggaat cggcttttcc gtagtcctcg
     4561 atgggtccga ccgcttctgg gtattcgtca ccgtcttgcg catcgtcgat gctggcagga
     4621 tccgagcagg cgatggctcc tagagcgaaa agcgcgaagc aggggcggca tgcgcgcggc
     4681 attttcaaag gcagtgtatt gacggtcatg gcgaatttcc ggctcaattg attttttgca
     4741 ccctaccatg gagggagaag aaaagcatca gcggcaaatt tccaggcgca accacttcat
     4801 ccgttccgag gctttcatgg tgatggggat ttcccgggca agtccggaac ccgtggtgca
     4861 aggtcctggc tcatcgttgt tcaggatatc caccatccga taggagccct gagccacgat
     4921 gccgagaagg gcggtcaatt cgccaggcaa cgcaaagctc tgggatacgt cctgattcca
     4981 gaggttgtgg aagacaaaaa cgacactccc cgcggtccat ttggcctggg cgaagaggcg
     5041 cgaatcgggg tcctggggtg tgttgcgcgg gcgcaagaag taatgattgg cggaaagcaa
     5101 ggcttggttg gctggctcca ggcgggcgcg attcatggcg ccgtaggatt gcacgaggga
     5161 ttggccctgc gggtgaaatt gctcggtgcc ttcgaaccgg cttggcatga acgcggattt
     5221 tcggaatccg aggccccagg gctctccgaa ctcctggccc atcaatagca
//
LOCUS       DAOSOR010000424         5270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15768_length_5270_cov_4.236242, whole genome shotgun sequence.
ACCESSION   DAOSOR010000424 DAOSOR010000000
VERSION     DAOSOR010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15768_length_5270_cov_4.236242"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PK710_18745"
     CDS             <1..244
                     /locus_tag="PK710_18745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11823.1"
                     /translation="SYCTGFGYASGTENFAEMYRWFYQGRETRELLEQHFEPCHRLLE
                     SSNGAWKAPWDNGMGEISSYRKTLWDSCDGTACRAW"
     gene            332..985
                     /gene="trxA"
                     /locus_tag="PK710_18750"
     CDS             332..985
                     /gene="trxA"
                     /locus_tag="PK710_18750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOT11824.1"
                     /translation="MALNDSIPTVDERNFEEEVLLCELPVMVDFMATWCGPCKTMAPE
                     FEALARELEGKAKLVKVDIDRSPALAGQLRIQSVPTTMVFAGGRLVNGKVGAMRKAQL
                     MQLIEPFLPRAQGALSPVEAAQLLQQGQIVLVDTREAPVYARAHLPGAVNMPLEEIRG
                     RLAELHMLAGVPVLYCRGGDKSKALAEELANDGIGVGFLEGGVLGWEAEGLPVDRPD"
     gene            1039..2688
                     /locus_tag="PK710_18755"
     CDS             1039..2688
                     /locus_tag="PK710_18755"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT11825.1"
                     /translation="MRSVVSRPLSPRAMAQAFALLTVLGLLTCHFHQAWAQRKTLSGT
                     WSATAMTERFNVGEWGPKCGPRPGGQGAPGGTVTITQQGNELDIRGAGRSYSTTNCWE
                     QAPGLQRVSHSASARGWSNRCATAANDPRQATVVTTISATDDAIHFDETGSYQFMLEG
                     QACTASVRRTRSFRLIQRQGEAAVGATPPAASSVASAPPTTSATPPAVAKTVDDGIKN
                     CASPGEPARLEVRPARKLLRAGDSFSFSARVVDEQGCPASVVPTWRVVQGTAKLSVSS
                     TGKVTAEENSPEGTADIQVSVGGKSVRVSVEVASRERYESLLEIRGLNELGETDEVAT
                     AMIATTSLGSGTAVAEDTAKKRKTIFAVVVGGGALLVVLVGLFFLRRGTRNRRVESDE
                     PMAPRVVPKVPLSERMVVGAPPEARSRQRHEVFEVDRAVQDKPPPVVCPLCKQEYPAG
                     SLFCPNDGTRLVKVAAISGAPKAATGICPTCERAFPLEQKNCPQHQQPLIPAPLFNAS
                     SLKRAGVEVKQGKVCPTCGSRYGVEATFCGKDGTALMLVNS"
     gene            complement(3095..3844)
                     /locus_tag="PK710_18760"
     CDS             complement(3095..3844)
                     /locus_tag="PK710_18760"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT11826.1"
                     /translation="MNRAKTANRLDVGLALTLLVIAWLMALTAPDELRGPWDLLSHGA
                     RSWTVVVLPAASLATILGIPLGIVSTLITRTHARLITWLLEILGGFPSIILVALLRSL
                     TIQSDYALPISIIALMAVRIPETVCYVRTQSLRFRSSESYLATYALGAGPARLLFNHL
                     LPSNAPALISMTFINVGVLTGIESAMAFVGLDATQSSWGTQIGHAIAQGHIRETLTPM
                     IALMATLFATYRLAETARRRMSPNTMRPTDG"
     gene            complement(3841..>5270)
                     /locus_tag="PK710_18765"
     CDS             complement(3841..>5270)
                     /locus_tag="PK710_18765"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT11827.1"
                     /translation="FFWLLTLVAPTPDPSLPRFFNLYPRDIKTLTDRAVRSLAEGPNE
                     GAQRELVRLGGAAFPHILPLLHPQPPVRQPIDTSPSPLDSQTQGRIAMALEPVAKRMG
                     LTDPSAFSSPSNAIAFWNRFWEERSVEFQPAAVRRAVHRQAAHGSPARLTTLIELDTF
                     ALQGIMQALDTCHTPDDVARSVRLLSVAAHVTQRDACIHPRMDPHQANRCILVWRDWW
                     LSSRFDYEPLSGPERLFAILTETQYGRWALEASTFQLGVDANGMTTFARFRYQGPSTF
                     ALAFFGLWLAYALAFPMSLAGALHHERTVDKISSGVLLALLTAPVALICFILAWIISN
                     PIAIAITAIGTTLVVAPARHQRIAALDSLSNPCLQYAAARGIPRSQIVLFHIGKPSLA
                     LAVTLFAFDFPIALSAACVAEQALGLPGLGHHLIDAVIERDIPLLMAMGLIGMTVHAL
                     MMFLGSLLGNWLDPRLDRSVHGEYP"
BASE COUNT         1120 a         1264 c         1835 g         1051 t
ORIGIN      
        1 gtcgtattgc acggggtttg ggtatgcctc cggaaccgag aatttcgcgg agatgtaccg
       61 gtggttttac caggggcgag agacgcgcga actgttggag cagcatttcg agccatgcca
      121 tcggttgctc gaatcatcga atggggcatg gaaagcgccg tgggacaacg ggatggggga
      181 gatatcctcg tatcgcaaga ccctgtggga cagttgtgac ggcacggctt gtcgggcgtg
      241 gtgagaggct ttccctggct cgatcacggc ttccccttgc atttggcacg gtggatgacc
      301 aaatggaggc tcgattctgg aaggagatcc gatggcattg aacgattcga tcccgacggt
      361 ggatgagcgc aacttcgagg aagaagtgct gttatgcgag ttgccggtga tggtggactt
      421 catggcgacg tggtgtgggc cgtgcaagac catggctccc gagttcgagg cgttggctcg
      481 ggaattggag ggcaaggcga aattggtcaa ggtcgatatc gaccgttcac cggcacttgc
      541 gggtcaactc agaatacagt cggtgccgac cacgatggtg ttcgcggggg ggcgattggt
      601 gaacggcaag gtgggagcga tgcgaaaagc gcagctcatg caactcatcg agccttttct
      661 tccgcgcgcg caaggggccc tttcccctgt cgaagcggct caactcctgc aacaagggca
      721 gatcgtcctc gtggatacgc gggaggctcc tgtgtatgct cgcgcgcatc ttcctggcgc
      781 ggtcaacatg ccgctcgaag agatccgagg gcgactggcg gaactgcaca tgctagcggg
      841 cgtgcccgtg ttgtattgtc gtggcggcga taaatccaaa gcgctagccg aggagctggc
      901 caacgacggg atcggggttg ggttcctcga aggaggggtg cttggttggg aagccgaagg
      961 gcttccggtg gaccgtccag actgaggttc aaccttttgc ttcggccttg ccgaaacatt
     1021 ttttggtagg cttggatcgt gcgctccgtt gtgtctcgtc cgttgtcgcc acgtgccatg
     1081 gctcaggcat ttgcccttct taccgtgttg ggcttgctga cctgtcattt tcaccaagcc
     1141 tgggcacagc gcaaaacatt gagcggaaca tggtctgcga cggccatgac ggagcgattc
     1201 aacgtcggcg aatggggacc gaagtgcggt cctcgtccgg gaggccaagg cgctccgggc
     1261 ggtacggtta ccatcacgca acagggtaat gaactggaca ttcgaggcgc gggacgctcc
     1321 tacagtacga ccaactgttg ggaacaggcg ccggggcttc aacgggtatc gcattccgcc
     1381 tccgcccgag gatggtccaa tcgatgtgcg acggcggcca atgatcctcg acaagccacg
     1441 gtcgttacaa cgatttccgc aacggatgac gcgatccatt tcgacgaaac cggctcgtac
     1501 caattcatgc tcgaaggcca agcatgtact gcctcggtac gtcgtacacg ctcgtttcgc
     1561 ctgatacagc gacaagggga agctgctgtg ggcgcgacgc ctccggcggc ttcatccgtt
     1621 gcttctgcgc cgcccacgac ttctgctacc ccacctgcgg ttgcgaaaac ggtcgacgat
     1681 ggaatcaaaa actgcgcctc gcctggtgag cccgcccgtc tcgaagtgcg tccggcgcgc
     1741 aagctgcttc gagcaggcga ttccttttct ttttccgcaa gggtggtcga tgaacaagga
     1801 tgccccgctt ccgtcgtacc cacgtggcgt gtggtgcaag gaaccgccaa actctccgtc
     1861 tcctccaccg gaaaagtaac ggcagaggaa aactcaccgg agggaaccgc tgatatccag
     1921 gtcagtgtgg gcggcaaaag cgttcgggtt tccgtcgaag tcgcttcgcg agaacgctac
     1981 gaatcgttgt tggaaattcg tgggctcaac gaattgggag aaaccgatga agtagcgacc
     2041 gcgatgattg ccacgacgag cttgggaagt ggaactgccg tggcggaaga tacggcgaag
     2101 aagcgaaaga cgattttcgc ggtggtcgta ggggggggcg ccttgttggt cgtgctggtc
     2161 ggtttgttct ttttgcgaag gggaactcga aaccggcgcg ttgaaagcga cgagccaatg
     2221 gcgccacggg tcgtgccgaa agtgccgcta tccgaacgca tggtcgttgg cgctccacca
     2281 gaagccaggt cgagacaacg gcatgaggta ttcgaagtag accgtgcggt tcaagacaaa
     2341 ccaccgccgg ttgtgtgccc cttgtgtaaa caagagtatc ccgcgggaag tctgttttgc
     2401 ccaaacgatg gaacgcggtt ggtcaaggtt gccgcgatca gcggggcccc gaaggcagcc
     2461 acgggaattt gtccaacgtg cgaacgcgct tttcccttgg agcaaaaaaa ctgcccacaa
     2521 catcaacaac ctttgatacc agctccgttg ttcaacgctt caagcttgaa gcgtgcgggt
     2581 gtcgaggtga agcaaggcaa agtctgcccg acctgcggct cccgttacgg agtggaagcc
     2641 actttttgcg ggaaagacgg cacggctttg atgctcgtca actcctgata cgcaacccac
     2701 cacgacgtcg ctgacagcgt gattgtcgaa ctggggggtc gtccatgggc ggacggggga
     2761 gagcatcaag ggcggccaaa tggtgtgggg ttcgataaga ggcgattgcg tgggacggtc
     2821 tattcgagcc atagcggtca tgacgaccac acgccgaggg cttgagattt aaaataataa
     2881 aatcaaatag ttatagaggg ttgatgttgt tgttccattc tcttcgacgc ctcgattcgc
     2941 gccccggcca tcttgaaatg ctcgtttggc ttgaaggatt ggaaaagaaa ccggcgagcg
     3001 gtggaagggc atacggttta ccagtccttg gagttgatgt tgggaaaggg ggcgatggct
     3061 gggggtgatg ggggagaatt ggcgcagtgg tgtttcaacc atcggtgggg cgcattgtgt
     3121 ttggggacat cctacggcgt gctgtttccg cgaggcgata ggtagcgaat aaggtggcca
     3181 tgagggcgat cataggggtg agggtttcgc ggatatggcc ttgggcgatg gcatgtccga
     3241 tttgtgtgcc ccacgacgat tgggttgcat cgagtccgac gaaggccatc gcggattcaa
     3301 tgcctgtcag aacgccgaca ttgatgaatg tcatgctgat cagggctgga gcgttggatg
     3361 gcaacaagtg attgaagagc agacgagcgg gtccggcgcc gagggcgtag gttgccaagt
     3421 atgattcgga ggagcgaaag cgaaggcttt gtgtgcgaac gtagcaaacg gtttcgggga
     3481 tgcggacggc catcaaggcg ataatcgata tgggcagggc gtagtcggat tgtatggtca
     3541 gagatcggag cagggcaacg aggatgatac tggggaatcc tccgaggatt tcgagcagcc
     3601 aggtgatgag gcgtgcatgg gtgcgagtga tgagtgtcga gacgataccc agagggattc
     3661 cgaggatggt tgccaacgat gcggcgggga ggactacgac ggtccaggat cgtgctccat
     3721 gggacagtag gtcccacggg cctcgaagtt catcgggagc ggtcaaggcc atgagccatg
     3781 cgatgaccag cagcgtcaac gccaatccta catcgagccg atttgcggtt ttcgctcgat
     3841 tcatgggtat tctccatgga cggagcggtc gagacgcggg tcgagccagt ttccaagcaa
     3901 ggaacccagg aacatcatga gggcatgaac ggtcatgccg atgagtccca tggccatcag
     3961 caaggggata tcgcgttcga tgacggcatc aatcaggtgg tggccgaggc cgggaagacc
     4021 gagggcttgc tctgcgacac atgcggcgga tagggcgatg gggaagtcga aagcaaatag
     4081 ggttacggca agggcgaggc tgggttttcc gatatggaat aacacgatct gggagcgagg
     4141 gatccctctg gcggcagcat attgcaaaca agggtttgac aaagagtcga gggcggcgat
     4201 gcgttgatgc cgtgcgggtg ctaccacgag cgtcgtgccg atcgctgtga tggcgatggc
     4261 gatgggattg gatatgatcc aagcaaggat gaagcagata agggcaacgg gggcggtgag
     4321 aagtgcgagc aacacaccag agctgatttt gtcgacagtg cgttcgtggt gcagggcgcc
     4381 cgcgaggctc atggggaagg cgagggcgta ggcgagccag aggccgaaaa aggcgagagc
     4441 gaaagtgctg ggaccttggt agcgaaaacg cgcaaaggtg gtcatcccat tggcatcgac
     4501 accgagttga aacgtggagg cctcgagggc ccagcggcca tattgtgttt cggtaaggat
     4561 ggcgaagagg cgttctgggc cggatagggg ttcataatcg aatcgggagg agagccacca
     4621 atcgcgccat acgaggatgc agcgattggc ttgatgtggg tccatgcgag ggtggataca
     4681 ggcatcgcgt tgggtgacat gggctgcgac gctcagcaat cgcacggagc gagcgacgtc
     4741 atcgggggtg tggcaggtgt ccagggcttg catgatgccc tggagcgcga aggtatcgag
     4801 ttcgatgagg gtggtgagtc gtgctgggga gccatgggct gcttgtcgat ggacggcgcg
     4861 tcggacagcg gcgggttgga attccacgga tcgttcttcc cagaagcgat tccagaaggc
     4921 gatggcgttg ctgggagagg agaaagcgga ggggtcggtg agtcccatgc gtttggcgac
     4981 gggctcgagg gccatggcga ttctgccttg cgtttgcgaa tcgaggggcg agggggaggt
     5041 atcgatgggt tggcgtacag ggggttgtgg atgaagaagc ggcaggatgt gtgggaaagc
     5101 ggcgcctccc aatcgaacca gttcgcgctg ggcgccttca ttggggcctt ctgccaacga
     5161 acggaccgcg cgatcggtga gtgttttgat gtcccgggga tagagattga aaaaccgtgg
     5221 cagggaggga tcgggcgtgg gggcgacgag ggtaagcagc cagaaaaaca
//
LOCUS       DAOSOR010000425         5269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15773_length_5269_cov_3.785002, whole genome shotgun sequence.
ACCESSION   DAOSOR010000425 DAOSOR010000000
VERSION     DAOSOR010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15773_length_5269_cov_3.785002"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PK710_18770"
     CDS             <1..629
                     /locus_tag="PK710_18770"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOT11828.1"
                     /translation="GGTGATGGTGATGGTGATGGTGGSAGASGGSPDLVINEIDYDQP
                     GDDTAEFIEIFNRSATAVDLTGKVLIFVNGGVTPPKEYARIPMSGTLTAHGYWVAKMD
                     PVTVPAGVMTEKITISVQNGPSDGVALFDTSTQTLIDAFCYGGPVLGAVFNGIPGTWD
                     LVEGTATTVKDSNKDVLSLIRQPNGQDTDNASADWMTTSTLTPGAPNP"
     gene            complement(848..1618)
                     /locus_tag="PK710_18775"
     CDS             complement(848..1618)
                     /locus_tag="PK710_18775"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT11829.1"
                     /translation="MRSERIEFPGSQGTLLAGRLEWESSTPNGWALLAHCFTCSKDIK
                     AAVTIAHRLVAAGFGVLRFDFTGLGGSEGDFSNTNFSSNIDDLVLAADWLRAERGTPA
                     LLIGHSLGGAAVIAAAHRIADSKAVATVGAPFDPGHIKRHFGPHLEQIEKAGSAVVQL
                     AGRPFTVRRQMLEDLKEHHQQQRLRTLNKPILVLHAPRDEVVSIEHAGRIFQAAMHPK
                     SFVSLHDACHLLRQPRDARYAADVIASWVSRYLDSSRS"
     gene            2138..3280
                     /locus_tag="PK710_18780"
     CDS             2138..3280
                     /locus_tag="PK710_18780"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT11830.1"
                     /translation="MPWRDLQRIVFRTALWVVGMSGCVFGGCDNDEKPTITGNPTTWK
                     ILLWAAGSTSEPTAMAAPLVEPDDGKMPETLKDQREVLFRRMKAYFGYTEETMAAVRQ
                     IVEGSSILGQGNPSETHPAMTRGECFQIRKAAGLSYLDDPICGMRNMVPLYKASEGEK
                     PQDARVCIDQYEFPNMPCEYPVVHVTTREAALLCKALGKRLCDAHEWEGACAGSLRDP
                     KDEYAFGRSRRDMKSIHNNNRTLVWAYGKEKNHALCATGSKKNKDCQGGGYRKCGSNT
                     YPAGSFPKCVSPLGVFDQHGNAAEHMNLPMKPDQLASLGHYGETEMKGSWFIFGTYEA
                     HEDDCHWRAPDWHATSVLSTGSHSNYHLGFRCCANVGGKPSEAASK"
     gene            3308..4504
                     /locus_tag="PK710_18785"
     CDS             3308..4504
                     /locus_tag="PK710_18785"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOT11831.1"
                     /translation="MKFFVRTFGCKMNLLDTARVVASLREAGHEQVQRAEQADMVLVN
                     TCTVTSESERKSRRAVRSAQRDGQRVAVFGCGPRAHGEAWKQAVGGAQICGSEAELLS
                     LFGAKPAPRASITTLPTRLLVPIQYGCDNGCTFCATRLARGAHRTVPREEVLETVRAF
                     ADSGGGEVVLSGINLAAWGCSISTHPWESELASLLTAILEQTTIERIRLSSLGPEYLD
                     PRFFKVFRDERICDHLHLSVQSGSFPVLQAMGRNYTVQDVEEIARQARRVRPDTAITA
                     DFIVGFPGETEEYFLQTLELVERVQFARLHVFPFSPREATAAYSYPDQVSEEDKRRRT
                     SLLTELGHACSARFHRSQQGKTVRALKLADGTSLTSHGLRLRTTTGRQGQWVQVRIEQ
                     KDIVSG"
     gene            4606..5220
                     /locus_tag="PK710_18790"
     CDS             4606..5220
                     /locus_tag="PK710_18790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11832.1"
                     /translation="MASLRPEPSMKTTSSAVESWAIKISFGFMLLGMFGLVVYDLVDP
                     ETAGAATGAEDSKKEADTAAATEAPAPSPSPTMDRRRSSTPAMERRSPMRISRERSLS
                     PPSAESEPLADPEEASEKSSISNARVMAERANRQPMISLGGSGERIRNLIRPIQAIGQ
                     PSEPSEERGGPAPEAPQDQPGSSPSQVPEAPEPGPPPEGAQGEH"
BASE COUNT         1156 a         1348 c         1688 g         1077 t
ORIGIN      
        1 cgggaggtac cggagccacg ggcggtacgg gagctacggg aggtaccgga gccacgggcg
       61 gtacgggggg aagcgcgggt gcgagcgggg gaagccctga tctcgtcatc aacgaaatcg
      121 actacgacca gccgggcgat gacaccgcgg aattcatcga gatcttcaat cgttccgcca
      181 ccgccgtgga tctcaccggc aaagtcctta ttttcgtcaa cggtggtgtc acgcccccca
      241 aagaatatgc tcgcattccg atgtccggca cgttgacagc ccatgggtat tgggtcgcca
      301 aaatggatcc agtcaccgtg ccggcgggcg tcatgaccga aaaaatcacg attagtgttc
      361 aaaacggacc gtcggatggg gtggcactat tcgatacttc cacgcaaacc ctcattgacg
      421 cgttttgcta tgggggacca gtgctgggag ccgtattcaa cggtatcccc gggacctggg
      481 atctggtgga aggtacggcg acaaccgtaa aagactcgaa taaagatgtg ttgagcctga
      541 ttcggcagcc caacggccaa gataccgata atgcttccgc ggattggatg accacgtcca
      601 cgctcacccc tggggcgccg aatccttgag gatttgtcgg aaccggggtg ggcaattcga
      661 atgtaatcta ttgatttaaa agagatttgg gctatttttt gctgcagggg tgtcgttgtt
      721 gtctacgacg tagtactgcc cggccagcgg atttggctca cgatgggcta ccctatcgat
      781 atgcaggatt tctgggatgc ggagggggga ttggaattct gattctaatt agaattagga
      841 actaaaacta ggaacgggag gaatcgaggt aacggctgac ccagcttgcg atgacgtccg
      901 cggcataacg ggcatcgcgc ggctgtcgaa gcagatggca ggcgtcgtgt agggagacga
      961 atgacttggg gtgcatggcg gcttgaaaga tccgtcccgc gtgttcgata ctcacgacct
     1021 catcgcgcgg agcgtgaagc acgagaatgg gtttgttcag cgttcgcaaa cgctgttgct
     1081 gatgatgctc ctttagatcc tcgagcattt ggcgtcgaac cgtaaaaggg cgcccagcca
     1141 attgcacgac ggcagagcct gctttttcga tttgctcgag atgcgggcca aagtgacgct
     1201 tgatatggcc tggatcgaac ggggcgccga cggtagccac ggctttggag tctgctatgc
     1261 ggtgggccgc ggcgatgacg gcagcaccac cgagcgaatg gccgatgagc aaggcaggcg
     1321 tgccacgctc ggctcgcaac cagtcggccg caagcacgag gtcatcgata ttggacgaga
     1381 agttggtatt ggaaaaatcg ccttcactgc ctcccagacc cgtaaaatca aagcgaagaa
     1441 caccaaaccc ggcagctacc agcctgtgcg cgatcgtaac cgccgctttg atatctttgg
     1501 aacaggtgaa acaatgggcc aacagtgccc aaccgtttgg ggtcgacgac tcccattcga
     1561 gacgacccgc caggagcgtc ccctggctac ccggaaactc gatgcgttcg ctccgcatgc
     1621 gactcactcc tttgggcaag tggccatagc agaaagaaag agtgcgtcca gcctcgcacc
     1681 atgcgcgcct gacggctcca aaggagtcga aatcagggcc ttttagcttc ggatgccgtc
     1741 ggagacgaaa agccaaaaga ccgaacgaac gggaaacaac ccctgggggc aactaaattt
     1801 atcgttgttt atcaattagt tacaacgatt tcgccccctg tccgggagca tggaaaccta
     1861 aagggagtca gggccatcgc cgccgccgca cagccttctt ttcccgcttg gctcccacat
     1921 atttggaata atccttgtcg taagccaatc gacataccat acaatccttt atttatagat
     1981 tatcgttatt caatcgcatc attgaaaacc tcgttgacga ggatagccat cggggcggtt
     2041 tcggggcagg agcaacgcgg aaagagagtc ggcacaggcg ttcgtcaggt agcgccgctc
     2101 tggacggcaa aggtgccaat ggctatagca ccgggcgatg ccgtggcgag acctccaacg
     2161 aatcgtgttt cgaaccgccc tttgggtcgt ggggatgagc ggttgtgtgt ttgggggctg
     2221 cgataatgat gagaagccga cgataacggg caacccgacg acgtggaaaa tccttttgtg
     2281 ggccgcggga agcacgtcgg agcctaccgc aatggcggct cctctggtgg agcctgatga
     2341 tggcaagatg cccgagacgc tcaaggacca gcgcgaagtg ttgtttcgtc ggatgaaggc
     2401 ctactttggc tatacggagg aaacgatggc ggcggtgcgg cagatcgtgg agggatcgtc
     2461 gattcttggg cagggcaatc cgtcggagac gcatccggcg atgacacggg gagagtgctt
     2521 tcagatcagg aaagcggcgg gattgagtta tttggacgat cccatttgcg ggatgcgcaa
     2581 catggttccg ttgtataaag cgtcggaggg agagaagccg caagatgcgc gggtatgcat
     2641 cgatcaatat gaatttccga atatgccgtg tgaatatccg gtggttcatg tgacgacgcg
     2701 ggaggccgcg ctgttgtgca aggcgttggg caaacggtta tgcgatgcgc atgaatggga
     2761 aggggcgtgc gcgggcagtt tgcgagatcc gaaagacgaa tatgcgtttg gccggtctcg
     2821 tcgcgacatg aagtcgattc acaacaacaa tcgcacgttg gtgtgggcct atggcaagga
     2881 gaagaaccac gctttatgtg cgacgggcag caagaaaaac aaggattgtc aaggaggggg
     2941 ataccgcaag tgcggttcga acacgtatcc tgcgggctct tttcccaagt gtgtgagccc
     3001 gctgggggta ttcgatcagc acggcaacgc ggcggagcac atgaatcttc cgatgaagcc
     3061 cgatcagctc gcgagtctgg gtcactatgg ggagacggaa atgaagggga gttggttcat
     3121 ttttgggacc tacgaagccc acgaggatga ttgtcattgg cgcgcgccgg attggcatgc
     3181 gacgagcgtg ttatcgacgg gcagtcactc caactatcat ctcgggtttc ggtgttgtgc
     3241 caacgtgggg ggaaagccgt cggaggctgc ttcgaagtag acggatatcg tggagggaag
     3301 cctcggagtg aagttttttg ttcggacttt tggatgtaag atgaacctgc tggacacggc
     3361 gcgggtcgtg gcatcgttgc gtgaagcggg tcatgagcaa gtgcagcggg ccgagcaggc
     3421 ggatatggtt ttggtcaaca cctgcacggt gacgtcggaa tcggagcgca agtcacggcg
     3481 tgcggtacgg tcggcgcagc gggacggtca acgggtggcg gtattcggct gcggtccgcg
     3541 agcgcatggg gaggcctgga agcaggcggt tggcggcgcg cagatctgtg gctcggaggc
     3601 ggagttattg agcctttttg gggccaaacc agcacctcgc gcgtccatca ccacgcttcc
     3661 cacgcggctg ctggtcccga ttcaatacgg ctgcgacaat ggttgtacgt tttgcgcgac
     3721 tcgtctggca cgcggggccc atcggacggt tccacgcgaa gaagtgctgg agacggtgcg
     3781 ggcgttcgcg gattcggggg gcggtgaagt ggtgctttcg ggcatcaatc tcgccgcctg
     3841 gggatgctcc atatcgacgc atccgtggga atcggagctg gcctcgttgc tcaccgccat
     3901 cttggagcaa acgaccatcg aacggatccg gttgtcatcg ttggggccgg aatatctaga
     3961 tcctcgtttt ttcaaggtat ttcgcgacga gcgtatctgt gaccaccttc atctttcggt
     4021 gcaaagcggg tcgttcccgg tgctgcaagc catgggtcgc aactacaccg tgcaggatgt
     4081 cgaagagata gcgaggcaag cgagaagagt tcgtccggat acggcgatca cggcggattt
     4141 catcgtaggt tttcccgggg aaacggaaga atattttctg caaacgctcg aattggtgga
     4201 acgagtgcag tttgcccggc tgcatgtctt tccattttca ccgcgagaag caacggccgc
     4261 ctacagctac cccgatcagg tatcggaaga ggataagcgt cggaggacat cgctgctgac
     4321 ggaactcggc cacgcgtgca gcgctcgatt tcaccgatct caacagggca aaacggttcg
     4381 tgccttgaag cttgcggatg gaacgagctt gacgtcccat ggccttcggc tgcgcaccac
     4441 cacgggacgg caagggcaat gggtacaggt gcgcatcgag cagaaggaca tcgtttccgg
     4501 ataaaaatcg attggttcgt cgagcgcccg acgaaatgcg cttgcgcttt ttgccattgg
     4561 tgtaagcttt gaatcgtctc gagcttgatg ggagcgaacg tcgtcatggc gtctcttcga
     4621 ccagaacctt ccatgaaaac cacttcctcg gcggtggagt cctgggccat aaaaatatcc
     4681 tttggtttca tgctgttagg catgttcggg ctcgtggtct acgaccttgt ggatcctgaa
     4741 accgcggggg cggccacggg agccgaagat tcaaaaaagg aagcggatac agcggcagcc
     4801 acagaggcgc ccgcaccgtc cccctcgcca acgatggatc gacgccgttc atccactcca
     4861 gccatggaac gtcgttcacc gatgcggatt tcgcgagaaa ggtctctttc accaccgtca
     4921 gcggagtcgg aaccgctggc ggaccctgag gaagcgtcgg agaagtcatc gatttcgaat
     4981 gcgcgggtga tggcggaacg agcgaatcgt caacccatga tatcgttggg gggttcaggc
     5041 gagaggattc gcaatctgat tcgtcccatt caggccattg ggcaaccgtc ggagccttcg
     5101 gaagaacgtg ggggacctgc tccggaagcg ccgcaagatc agcctgggtc atcgccttcc
     5161 caagtgccgg aggcgccaga gccgggccca ccgccggagg gagcgcaagg ggagcactga
     5221 ttctttcggc atggtatcga ccggcatgat gtcgaggcat cgacaacgg
//
LOCUS       DAOSOR010000426         5260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15810_length_5260_cov_4.528146, whole genome shotgun sequence.
ACCESSION   DAOSOR010000426 DAOSOR010000000
VERSION     DAOSOR010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15810_length_5260_cov_4.528146"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PK710_18795"
     CDS             complement(<1..505)
                     /locus_tag="PK710_18795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11833.1"
                     /translation="MAFFSSPKSWLPGLMAVVVATPVTMVTPEASASPTPNTADASLP
                     PALRNYPGVTRIEHHDGKIRVEVSQSFAESLDDLRHEQWLSGLFGQCDAVGLPVTGIW
                     EEVRTAPGKLEPLEKWLSHPVLPTHLRGAGSMTPRGASQVKHYPLPLPYGGGLVGTAM
                     VAAAKLGA"
     gene            719..1549
                     /locus_tag="PK710_18800"
     CDS             719..1549
                     /locus_tag="PK710_18800"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOT11834.1"
                     /translation="MSEGAIREAARVIQQADALFITAGAGMGVDSGLPDFRGDEGFWR
                     AYPPFKARGLTFVDMANPSWFERNPAVAWGFYGHRLALYRQTLPHEGFRILRRWAENM
                     RFGAFVFTSNVDGQFQRAGFSEDAIVECHGSIHHFQCTVPCHEQIWEADAPITVDPST
                     FEASEPLPRCPRCGKLARPNVLMFGDWNWIADRTSAQERRWDEWLEEQRGASLAIVEM
                     GAGSAVPTVRRTSEQIGRRKGATLIRINPREPEVPSGHLGIAGSAKAILQQIEECMRA
                     "
     gene            complement(1555..1824)
                     /locus_tag="PK710_18805"
     CDS             complement(1555..1824)
                     /locus_tag="PK710_18805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11835.1"
                     /translation="MQQLAAIEEALEIVRQTIELLASRKDDEAAFDIARAQYRSSIRD
                     SWPNNLSSLTDVLDRIHRDPGSKLDEAERARLAHAVHLLRNAMNQ"
     gene            complement(1894..2823)
                     /locus_tag="PK710_18810"
     CDS             complement(1894..2823)
                     /locus_tag="PK710_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11836.1"
                     /translation="MATMKTLSRWASDVRERNRKLYVTLRRGLDLEGVQAMLLGHDLR
                     NVAGLRIPGSVGLVGLRMLVSDSRLPNLVSLAIFGFEDADDAVDEVFESPIIHRLQVL
                     KVWSVSDTLQARLGRGKAKALRQLDIRYSRELTTLDRYFESPHVQTLRFLTLERTALS
                     DATMLFHNPAARQLQSLRLIGCDFDSETVDHLIVSPYMRNLRKLDLSNNTQDDVLASI
                     ESLLRTGTPPGLESLALRGNELMEFDWSRVKLPRLRVLDVRDNPMDLDELLEILQAPG
                     LPCLKKLIAHSPEGFLPANLDPRLILDDRKQKW"
     gene            complement(2934..3986)
                     /locus_tag="PK710_18815"
     CDS             complement(2934..3986)
                     /locus_tag="PK710_18815"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HOT11837.1"
                     /translation="MSRVDDSNHHPFDPNDATVFDGIFGLPHTLRDAYVVLIPVPFDA
                     TTSYRKGAAEGPQTILEASWQVDLWDAETGDPWKEGIFMLDEPEMIVRLNDEARLLAA
                     PIQNQEDEVLGSDSLERINAIGEQVMTWVHDEVVSWLDQGKLVGIIGGDHASPLGAIM
                     AIAERNPGLGILHIDAHADLRCAYQGFTHSHASVMHNVLSRVPEVQRITQVGLRDLCA
                     NEMQTIQTDKRLRSFFDHDLARAQFEGKNYASLVQQIVQTLPDKVYVSFDIDGLDPSL
                     CPGTGTPVPGGLFFHQASFLLGELVRSGRTIVGFDLCEVAPVEDSISIDGVVGARMLY
                     KLIGWSLLSQSPKRES"
     gene            complement(3973..4299)
                     /locus_tag="PK710_18820"
     CDS             complement(3973..4299)
                     /locus_tag="PK710_18820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11838.1"
                     /translation="MTKPAHIWIDRKQVIYHDDDRSFDRISFALRALRLVMPAEMTVA
                     VCEGRHAVWTDQGKNLRAGNDARWGILSVPPHASRVDIAIAVAQLAGKTEDPFIIDML
                     LHHEPS"
     gene            complement(4341..>5260)
                     /locus_tag="PK710_18825"
     CDS             complement(4341..>5260)
                     /locus_tag="PK710_18825"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HOT11839.1"
                     /translation="LAMRTAREVIGIGILSSLACSTLVLSARADLSSPPITAQHPVVF
                     ETPYKLGHRALPRETKAEAESRTRWNMGGKTGPWHPHPRVIVDNVRVKGAVSPASALR
                     LTRANGYWPIRRCYDPSLVEQPSLRGKVSIRVTVGASGKALRSSVVGKPTLADTQVTS
                     CIRDGLRGISYPATGKGKAEITFDVTVNPGDAPMKPVPDPPVSGGPGTLSLPIVQALV
                     ARHGGEAIQRCYEQAQRRVPGLWGRMVLRLDVAPNGAIREIVETDSTFPDEATTQCAI
                     DAIRGIGFPPPHGGDLRIIVPIRFGRPPS"
BASE COUNT         1135 a         1421 c         1591 g         1113 t
ORIGIN      
        1 gcgcgccgag cttggccgcg gccaccatgg cggtgcccac gagcccgccg ccgtaaggca
       61 agggcagggg atagtgtttg acctggctgg cgccgcgtgg ggtcatggag ccggctcctc
      121 gaaggtgggt ggggagcacg ggatgggaga gccatttttc gaggggttcg agcttgccgg
      181 gggcagttct cacctcttcc cagatacctg ttacgggaag gccaacggca tcacactgac
      241 cgaagagtcc cgaaagccat tgttcgtggc ggagatcatc cagggactcg gcgaaactct
      301 gggagacttc gactcgaatt ttgccatcat gatgctcaat gcgagtgacc ccgggataat
      361 ttcgaagcgc cgggggcagc gaggcatccg ccgtgttcgg agtgggcgaa gcggaagctt
      421 ccggagtcac catcgtgaca ggagtcgcca caacgacggc catcagccca gggagccagc
      481 ttttggggct tgaaaaaaac gccatgatgg actcgatagc acgtcttgta ccacgagaaa
      541 aatccctctg aatcctggga actcatggcg gttatcgggt tgggtggacg gcaggtatga
      601 tcctgctgat ctcatataga gaagtccgaa atgaccaatt ccgccccaaa agcacattcc
      661 atggtggcaa tggcctcgac aagctatgct gcaagcgtct tccacagcga gggaaaagat
      721 gtcggaaggg gccattcgcg aggcagctcg agtcattcaa caggctgatg cgcttttcat
      781 tacggcggga gccggtatgg gagtggactc tggtcttcct gattttcgcg gcgacgaagg
      841 tttttggcgg gcttatcccc ctttcaaagc gcgagggctt acgtttgtcg acatggcgaa
      901 cccctcctgg tttgagcgaa atcccgcggt agcctggggg ttttacggtc atcgcttggc
      961 gctataccgg cagacgctcc cgcacgaagg ttttcgcatc ctgcgtcgat gggccgaaaa
     1021 catgcgcttt ggggcttttg tattcacctc gaatgtcgat ggtcagtttc agcgagccgg
     1081 tttttctgaa gacgccatcg tggaatgcca cggttcgatt catcacttcc aatgtaccgt
     1141 gccttgtcat gagcagatct gggaagcgga tgccccgatc acggttgatc catcgacgtt
     1201 tgaagcttcc gaaccgttac cgcgttgccc tcgatgcgga aaactcgcgc gtcccaatgt
     1261 gttgatgttt ggagattgga attggattgc cgatcgaacg tcggcgcaag agcgacgatg
     1321 ggacgagtgg ctggaggagc aacggggcgc atcgctggcc atcgtggaaa tgggagcggg
     1381 ctcggcggtc cccacggttc gtcgaacgtc tgaacagata ggtcgtcgca agggggccac
     1441 gttgatacgc atcaatccac gggagccgga ggtgccaagc ggccaccttg gaatagcggg
     1501 ctcggccaaa gcgattctcc agcagataga ggagtgcatg agagcgtaag gtggttactg
     1561 attcatggca ttgcgaagca agtggacggc gtgggcaagg cgcgcccgct cagcttcgtc
     1621 gagtttggaa ccaggatcac ggtgaatcct gtcgaggacg tcggtcagcg agctgaggtt
     1681 gttgggccag gaatctcgaa tactcgaacg atactgcgcc cgggcgatgt cgaacgcagc
     1741 ttcatcgtct tttcgagacg ccaatagctc gatggtttgt cgcacgatct ccaacgcttc
     1801 ttcgatcgct gctagctgct gcatagggga gagtatgcct tttcggcggg atgatcggta
     1861 gggggtcgat aaggcaaacg aaagccgggg tttttaccac ttctgttttc ggtcatcgag
     1921 aatcagtcgt gggtccaggt tggctgggag gaatccttcc gggctgtggg cgatcagttt
     1981 tttcaggcaa gggagccctg gcgcttgcag gatttcgaga agttcatcga gatccatggg
     2041 gttgtcacgc acgtcgagaa cgcgcagacg aggcagcttg actctgctcc aatcgaattc
     2101 catcaattca tttccccgaa gggcaaggga ttcgagacca gggggggttc ctgtccgcag
     2161 caacgattcg atggaagcga gcacatcgtc ttgcgtgttg ttggacaggt cgagtttgcg
     2221 cagattgcgc atatacgggc tgacgatcaa gtggtccacg gtttcggaat cgaagtcgca
     2281 tccgatcaag cgcagggatt ggagttggcg cgcggcggga ttgtgaaaga gcatggtggc
     2341 atcggatagc gcggttcgtt cgagggtcag aaagcgaagg gtttggacat gggggctttc
     2401 gaagtaacgg tccaaggtag tgagttcgcg actgtatcga atgtcgagtt gacgcaaagc
     2461 cttggctttt cctcttccaa gacgagcctg cagcgtatcg gacacggacc agaccttgag
     2521 cacttgcaag cgatgaatga tcggggattc gaacacttca tcgaccgcat cgtcagcgtc
     2581 ttcgaaccca aaaatggcta atgaaacgag gtttggcaat ctcgaatccg aaacgagcat
     2641 gcgcaatccc accaatccga ccgacccggg gatgcgcaat cctgccacgt tgcgcaggtc
     2701 atgaccgaga agcatggcct gcaccccttc gaggtcgagc ccacgacgaa gggtgacata
     2761 tagctttcga tttcgctcgc gaacatccga agcccatcgc gagagagtct tcatcgttgc
     2821 catggtcccg ccgtaccacg aacagacctg gtgtcaacgc ttcgaagaca cgcgccgcga
     2881 gttccgcaag gaaacaacga tgagattacg ttagaaaaag ccgggatttg gcttcaactc
     2941 tcacgcttgg ggctttgcga tagcagcgac caaccgatga gtttgtagag cattcgcgca
     3001 ccgaccacgc catcgatgct gatcgaatct tcgacaggag ccacttcgca taagtcgaat
     3061 cccacgatgg tgcgtcctga gcgcacgagt tcgcctagca gaaacgaagc ttgatggaag
     3121 aacaggccac cgggaacagg ggttcccgta ccaggacaca gggatggatc gagtccatcg
     3181 atatcgaagg aaacgtagac cttgtcgggt aaggtttgca cgatttgttg aacgagtgat
     3241 gcgtagtttt ttccctcgaa ttgcgcgcgc gccaggtcat gatcgaaaaa gcttcgcaag
     3301 cgcttgtccg tctggatggt ttgcatttca ttggcgcaca ggtctcgcaa tcccacttgc
     3361 gtgatacgtt gcacctcggg cacccgggaa aggacgttgt gcatgacgct tgcgtgagag
     3421 tgggtgaacc cctggtaagc gcatcgcaag tccgcgtggg catcgatatg caaaatgcca
     3481 agcccggggt ttcgctcggc aatggccatg atggctccca gcgggctggc atggtcacca
     3541 ccgatgattc cgacgagttt tccctgatcg agccacgaaa ccacctcgtc gtgaacccag
     3601 gtcatgactt gttcgccgat ggcgttgatt cgttcgaggc tatcggaacc caatacctca
     3661 tcttcttggt tttgaatggg ggccgcgaga agccgtgctt cgtcattgag acgaacgatc
     3721 atttccggct catcgagcat gaatatccct tctttccaag ggtctcccgt ctctgcatcc
     3781 caaagatcga cttgccagct tgcctccaag atcgtctgcg gtccttccgc ggcccctttt
     3841 cggtacgacg tcgtggcatc gaaaggcacc ggaatcagaa cgacataagc atcccgcagc
     3901 gtgtggggaa gtccgaaaat gccgtcgaaa acggtggcat cgttgggatc gaacggatga
     3961 tggttggaat cgtcaactcg gctcatggtg caacaacata tcgatgatga aagggtcttc
     4021 ggtttttccc gcgagttgcg caaccgcgat ggcaatgtcc acgcgtgaag catggggagg
     4081 cacactcagg ataccccacc gcgcatcgtt tcccgcacga aggttctttc cttggtctgt
     4141 ccaaaccgca tgacggcctt cgcataccgc aaccgtcatt tctgcaggca tcaccagccg
     4201 caaggcccga agcgcaaaag agattcgatc gaaacttcga tcatcgtcgt gatagatgac
     4261 ctgtttgcga tcaatccaga tatgagcggg cttggtcacg tcgagcgaat catagtctcc
     4321 ccctgtcgat tcgccagacc ctacgacggc ggcctaccga atcgaatggg aacgatgatg
     4381 cgcaaatccc caccgtgcgg cggcggaaaa ccaatgcctc gaatggcatc gatcgcgcat
     4441 tgggtcgtcg cctcgtcagg aaatgtcgag tccgtttcca cgatctcacg aatggcgcca
     4501 ttgggagcca catccagacg cagcaccatg cgcccccaca gacctggaac tcttcgttgc
     4561 gcttgctcat aacatcgttg aattgcctcg ccgccatggc gtgccacgag agcttgcacg
     4621 atcggcaagg acaaggttcc cggtccgccc gaaaccggag gatccggcac gggtttcatc
     4681 ggggcatcgc cgggattgac ggtgacgtcg aaggtgattt cggctttgcc tttgcccgtg
     4741 gccggatacg atatccctcg caatccatcg cgaatgcacg aggtcacttg ggtgtcggcg
     4801 agagtgggtt tgcccacgac cgatgaccgg agagcctttc ccgaagcccc cacggtgact
     4861 cgaatggata cttttcctcg cagcgacggt tgttcgacca gggacgggtc gtagcaacga
     4921 cgaatgggcc aatagccgtt ggcgcgggtc aagcgcaagg cggaggctgg ggaaaccgca
     4981 cctttgacac gtacgttgtc cacgatgacc cgcggatgag ggtgccaagg gcctgttttc
     5041 ccgcccatat tccatctcgt ccggctctcg gcttccgcct tcgtttcccg cggtagtgca
     5101 cgatgtccca gcttgtaggg tgtctcgaat acgacgggat gctgggccgt gatcggtgga
     5161 gaagacaggt ccgcacgggc ggaaagaacc aatgtcgagc aagccaaact cgataggata
     5221 ccaatcccaa tcacttcgcg cgcggttcgc atggcgagac
//
LOCUS       DAOSOR010000427         5255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15832_length_5255_cov_3.877885, whole genome shotgun sequence.
ACCESSION   DAOSOR010000427 DAOSOR010000000
VERSION     DAOSOR010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15832_length_5255_cov_3.877885"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PK710_18830"
     CDS             <1..248
                     /locus_tag="PK710_18830"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11840.1"
                     /translation="DARERKLWDKYMHAYEDMLRHTSTHAAPWYVVPADKKWFTRVAV
                     SEIIVDRLSKLDLHYPKLTDQQIEDLKLIREQLLSND"
     gene            complement(399..1898)
                     /locus_tag="PK710_18835"
     CDS             complement(399..1898)
                     /locus_tag="PK710_18835"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOT11841.1"
                     /translation="MTTSEDSFQRIKPFVSNLERPVFAITGLPEEVIAVLFAYYSRSR
                     DDLRTNLARLISDDTLGIASSQSPVSCHAQEKARAFHEKWVVGYGHASVAEHAVVHLA
                     VEQVSIIASKVIEDLRLGSFTEKSTRYVVFDTGSFADLPSLPSPLAEPYRTACKNLFS
                     TYLSLFPKVEAKLERLFPAEAQASRTHRQAALRAHTCDLLRGLLPASTLTNLGITANA
                     RALSNLLSKMLSSPLAEVRSVAEQMRQEALTVTPTLLRHVAPNPYRQSLQPTLTPLTA
                     PSTQIADSPVAVRILRHDADALQRIVLALDYDQGAQPHAGRRWEMLQAKEVSALESMV
                     KRALAHRGPHDPAPRAMESTSITVELELDYGAYRDLQRHRMLTPSTQLLGCSLGYTPP
                     EELSALAIGDDYARALDAVQIPWQRLAQHDPFQAQYVVPLAYRTRVLWTLNLREVFHV
                     VELRSARQGHTSYRRVAQALYREICNIFPWLTELMRVDMQEHSLARGTM"
     gene            complement(1941..2738)
                     /gene="map"
                     /locus_tag="PK710_18840"
     CDS             complement(1941..2738)
                     /gene="map"
                     /locus_tag="PK710_18840"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOT11842.1"
                     /translation="MAIDIKSPREIEAIRTSCQMAADTLLKVGELLRPGMTTEQINTF
                     VHEDTIRRGAIPSPLNYHGFPKSVCTSINEVVCHGIPGPQKLQPGDILNIDITTFFNG
                     FHGDTSATFYIGEPSPESKHVTEVARRCLALGIAEVRDGARLGDVGAAIQEFAESQGC
                     SVVRQFVGHGIGRKFHEPPQVNHFGTRGKGERLRAGMVFTIEPMINLGGWEVEILDDD
                     WTAVTRDRSLSAQFEHTIAVTKTGCEILTQRNRTLTHSEIFPDFFAS"
     gene            complement(2784..3350)
                     /locus_tag="PK710_18845"
     CDS             complement(2784..3350)
                     /locus_tag="PK710_18845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11843.1"
                     /translation="MKKRSFRLRLPPLLLLFTTITMTTACSKQLPTDTPRSVAFWEGD
                     FQTLFDDSIDPAIVGLNPTASDTQPTYFTRGQTAQLASRARVTTVTVEGSKQAPQITM
                     VLRLMGPPIVGQPPSYDTVTITVSPQNRAFNLIRSNPMRLSGAQVIVFLREFDSPEGR
                     VTHWHLARDTQQTADAASRGALLGPRSD"
     gene            3562..4428
                     /locus_tag="PK710_18850"
     CDS             3562..4428
                     /locus_tag="PK710_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11844.1"
                     /translation="MNRISKWGLCVVLGFGVVAGSMVAADDAFAQKKKGGAAAAVAPA
                     APVDPPTVKNREGIEIRPAGLHWGMSSAQLIQFYERVIDRDYIPLYRKAQAGPQTARL
                     DAAVANTKAAFRRSKVEFGNLPTGVDSTPLKGEYTYQNGESMMHLNRRGAGSRYFFFI
                     QDKLWKIYDEVPLGEKRPLGPSYEEAVKRLATRYGVVGRVTQPDYAIGRNFMEVDWQD
                     TRTHLRVLDRSGLQIVGVVYEDRTTLANLASLRSNKPAEVGGVDPDVEALIRPPSAAP
                     GPVEEKDPKKKK"
     gene            4484..4765
                     /locus_tag="PK710_18855"
     CDS             4484..4765
                     /locus_tag="PK710_18855"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOT11845.1"
                     /translation="MDAARQRFIDLCRDVLRPLIEADEGKLYYVSITDRQVVLHLGGG
                     CCGCPGAPLTIRTLIEPAIHAIDPKVQVIVTVGAKRPDEAILIDAAFSS"
     gene            4898..5152
                     /gene="rpsP"
                     /locus_tag="PK710_18860"
     CDS             4898..5152
                     /gene="rpsP"
                     /locus_tag="PK710_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOT11846.1"
                     /translation="MAVHIRLTRQGTKKKPFYRIIVADQRSRRDGRFIEKLGTFNPLD
                     ASGMLQLNKDRLAYWLGQGAQLSHTVDRLIKTAPVVASES"
BASE COUNT         1132 a         1344 c         1613 g         1166 t
ORIGIN      
        1 ccgatgcccg cgaacgaaaa ctctgggaca agtacatgca tgcgtatgag gatatgctcc
       61 gccatacaag cacccatgcc gccccttggt acgtcgttcc cgccgacaaa aaatggttca
      121 ccagagtcgc cgtcagcgaa atcatcgtgg accgactctc caaactcgat ctgcactatc
      181 ccaagctgac cgaccaacag atcgaagacc tcaaactcat tcgcgaacag ctcctgtcga
      241 acgactgaac cttggacggt ttagctggcg cccaaaccaa taacgaaaca atcgcgacca
      301 tcccgcgtcc atgacccttt cctttctcat gacccacgag cgataactcc ctccgtatcg
      361 gatggtcccg ggcgatcaac catgaggata gcgtttgcct acatggtgcc tctcgccagc
      421 gagtgctcct gcatgtcgac gcgcattaat tctgtaagcc atggaaatat attacaaatt
      481 tctcgatata gggcctgggc cacgcgacga taactcgtgt gtccttgtcg cgcgctacgc
      541 aactcgacca catgaaagac ctcgcgcagg ttgagcgtcc acaacacgcg agttcgataa
      601 gcgaggggaa cgacatattg tgcttggaag ggatcgtgct gtgcaagacg ttgccagggg
      661 atctgaacgg catcgagggc gcgagcgtaa tcatcgccta tggccaacgc cgatagttct
      721 tccggagggg tgtatccgag ggagcaaccc agcaattgcg tgctcggggt tagcatccga
      781 tgtcgttgca agtcgcgata agcgccgtaa tccaattcca attcgacagt gatgcttgtc
      841 gactccatgg ctcgaggggc gggatcgtgg gggccgcgat gggccaaggc tcgtttcacc
      901 atcgactcca aggccgacac ttccttggcc tgaagcatct cccatcgtct gcccgcatga
      961 ggttgcgccc cttggtcgta atcgagagcc aggacgatcc gctgcaaagc atcggcatcg
     1021 tgacgaagga tgcgtacggc caccggtgaa tcggcaatct gtgtcgatgg agccgtgagt
     1081 ggggtcagtg tgggctgcaa cgattgacga taaggattgg gagcgacatg gcggagcaag
     1141 gtgggggtga ccgtcaacgc ctcttgtcgc atttgctccg ccacggaacg tacttccgca
     1201 aggggtgacg agagcatttt gctcagcaga ttggacagcg ctcgtgcgtt ggcggtgatg
     1261 ccgagattgg tcagcgtgct ggcggggagc aagccccgca atagatcgca agtatgcgcg
     1321 cgcaaggctg cctgtcgatg cgttcggctt gcttgtgctt ccgcggggaa gagcctttcg
     1381 agcttcgctt cgaccttcgg aaacagtgat aagtacgtgg aaaacaggtt tttgcaggct
     1441 gtccgatagg gttcggccag tggcgatggc agagagggta agtcggcaaa cgaacccgta
     1501 tcgaacacca cgtaccgcgt gcttttttcg gtaaacgaac cgaggcgcaa atcctcgatg
     1561 accttggaag cgatgatgga tacttgttcg acggctagat gaacgacggc atgttcagcg
     1621 acggaggcat gtccgtagcc gaccacccat ttttcgtgaa aggctcgcgc tttttcctgc
     1681 gcatggcagg atacggggct ttgggatgag gcaattccga gcgtatcgtc ggagatcaaa
     1741 cgcgcaaggt tggtgcgcaa gtcgtcgcgg cttcgggaat agtacgcgaa cagaacggca
     1801 atcacctctt cgggcaggcc cgtgatggca aaaacgggtc gttcgaggtt ggaaacgaag
     1861 ggtttgatgc gttggaagga gtcttcggag gtcgtcatgg tttggggacg gagcgtaggg
     1921 tagcgacggg ttttcgagaa tcaggaagcg aaaaaatccg gaaaaatctc gctgtgggtc
     1981 aacgttctgt ttcgttgcgt caggatttcg cagccggttt tggtgaccgc gatggtgtgt
     2041 tcgaattggg cggaaaggga gcggtcgcgg gttacggcag tccagtcatc gtcgagtatc
     2101 tctacttccc aaccaccgag attgatcatc ggttcgatgg tgaacaccat cccggcgcga
     2161 agacgttctc cttttccccg cgtaccgaag tggttgacct gggggggctc gtggaatttt
     2221 cgaccgatac cgtggcctac gaactgacgg actaccgagc agccttggga ttccgcaaac
     2281 tcctggatgg cggctcccac atcaccaagc cgagcgccat cgcgaacttc cgcgatgcca
     2341 agagccaaac accgtcgcgc cacttcggtg acgtgtttgc tttccggaga aggctcaccg
     2401 atataaaacg tagcggaggt gtctccgtga aacccattga aaaacgtggt gatatcgatg
     2461 ttgaggatgt ccccgggctg gagtttttgc gggccgggaa tcccatggca caccacttca
     2521 ttgatggacg tgcaaacgct tttgggaaag ccgtggtagt tgaggggtga gggaatcgcg
     2581 cctcgacgaa tcgtatcctc atgaacgaac gtgttgattt gttcggtggt catgccaggg
     2641 cggagcaatt ccccgacttt gagcagcgta tcggctgcca tttggcagct tgtacggatg
     2701 gcctcgatct cacgagggct tttaatatca attgccatgg gatagggggg gaatagggga
     2761 caatacttga ttccgtcaag ccatcagtcg gaacgggggc cgagaagtgc gccccggctt
     2821 gctgcatctg cggtttgctg ggtgtcgcga gccagatgcc agtgggtgac tcttccctcc
     2881 ggactatcga actcgcgtag aaaaacgatg acttgagcgc cggaaagccg catcggattg
     2941 gagcggatga ggttgaacgc gcgattttga gggcttacgg tgatggtcac agtgtcatac
     3001 gagggaggtt ggccaacgat gggagggccc atcaggcgca ggaccatggt gatctggggg
     3061 gcttgtttac tgccttcgac ggtgacggtc gtgacgcggg ctcgactggc gagctgggcg
     3121 gtttgtcctc gggtgaagta agtgggctgg gtatcgctgg ccgtcggatt gaggccgaca
     3181 atggcaggat cgatgctatc gtcgaacagg gtttggaagt ctccttccca aaaagcgacc
     3241 gaacgtgggg tatcggtggg aagttgcttc gaacaggcgg tggtcatcgt tatcgtggtg
     3301 aacagaagca ataagggtgg tagccgtaag cggaaggagc gttttttcat ggcaggcccg
     3361 agaaaagaaa acactctgga cggataccac ttggtttgga caggatcgaa agaaaagtga
     3421 catgggcttt ccatcaggtt tgtgcaatgg gcgtctaatg gatcgttgct tgagcacttc
     3481 ccggctgcag tgctagcctg ccactgtggt cagcaaaccg ttgtgatggt tgcgtcacgg
     3541 ccttgtcatg aggagatcac gatgaatcgc atttcgaaat ggggactgtg tgttgtcctg
     3601 ggatttggtg tggtagcggg gagcatggtc gcggctgacg acgcgtttgc ccaaaaaaag
     3661 aagggggggg ccgcggcggc ggtcgctccg gccgcaccgg tggatccacc gacagtgaaa
     3721 aaccgagaag ggattgagat acgtccagcg gggttgcact gggggatgtc gtccgcgcag
     3781 ctcatccagt tttacgagcg ggtcatcgat cgcgactata ttcctctgta tcgaaaagcc
     3841 caagcggggc ctcaaacggc tcgtctcgac gctgcggtag ccaatacgaa agcggcgttt
     3901 cgtcgcagca aagtcgaatt cggtaatctt ccgaccggtg tggattccac gcctttgaaa
     3961 ggcgagtaca cctatcagaa tggcgaatcg atgatgcatc tgaatcgacg aggtgctggc
     4021 tcgcggtact ttttcttcat tcaggataag ctttggaaaa tctatgatga ggttccgctt
     4081 ggggagaaac gtccgttggg accgagctat gaggaagcgg tcaaacggct tgctactcgt
     4141 tatggggtgg ttgggcgggt aacccagccc gattacgcga ttggacggaa cttcatggaa
     4201 gtcgattggc aggatacccg cacccatctt cgggtcttgg atcgttcggg ccttcagata
     4261 gtgggggtcg tgtatgaaga tcgcacgacg ttggccaatc tcgcctcgtt gcgttctaac
     4321 aaaccggcgg aggtgggagg tgtggatccg gatgtcgagg cgttgattcg cccgccttcg
     4381 gcggcaccgg ggcctgtgga agaaaaagac ccgaagaaga agaagtaacc tgattgatct
     4441 tatcgaccga agacaacttc gttgtggtag cctgagcgcc ttcatggatg ctgctcgcca
     4501 gcgcttcatc gacttatgtc gcgatgtgct tcgtccgttg atcgaggcgg acgagggaaa
     4561 gctgtactat gtgtcgataa cggaccggca agtggtcttg catttgggag gaggttgctg
     4621 cggatgtcct ggtgctcctc tgacgatccg cactctcatc gaacccgcca tccatgccat
     4681 cgaccccaag gttcaggtca tcgttacggt aggcgccaag cgtcccgacg aggccatcct
     4741 cattgacgcg gctttttcgt cttaggtttg ggatagcgaa ccaggggttg accaaagcgc
     4801 ttttccatga ctaccgttgg ccctcatttt tgacgggcta tttgccccgc ttgtgctaca
     4861 agcctgctcc cccttttggg atgacatagg acgacagatg gctgttcaca ttcgactcac
     4921 tcgtcaaggc accaagaaaa agccttttta ccgcattatc gttgccgatc agcgttcgcg
     4981 ccgcgatggc cgcttcatcg agaaactcgg taccttcaac cctctcgatg cttctggcat
     5041 gctccaactc aataaggatc gcttggcgta ttggctcggc caaggcgcac agctatcgca
     5101 cacggtggat cgtctcatca aaaccgcgcc tgtggtcgcg agtgaaagct gaccctccac
     5161 ttatttcgag tccctgatgg ctctacggac cctaatcgat accatcgtcc gatctctggt
     5221 cgaatatccg gacgaagtcg aagtcgctga gatcg
//
LOCUS       DAOSOR010000428         5241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15876_length_5241_cov_4.648284, whole genome shotgun sequence.
ACCESSION   DAOSOR010000428 DAOSOR010000000
VERSION     DAOSOR010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15876_length_5241_cov_4.648284"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1149)
                     /locus_tag="PK710_18865"
     CDS             complement(58..1149)
                     /locus_tag="PK710_18865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11847.1"
                     /translation="MYNHRQIRLRCSSALGVASLSVFLIWGCATGKDTEPDSWTPGAA
                     GGTNTAGASGFGAGADGGEQGGSAGESGTGGAGAGGGAAGVAGQAGGTGGGGTGGGGT
                     GGGGAGGAGGSAPTGGAAGSSGSGGASGGTGGGPGGTGGTTGGSGGGTPAPSCDDQIQ
                     NQGEEGIDCGGPCPTPCPSCSDQIQNQGETGIDCGGPCPPCGFTHEESFDYFPNWSPD
                     GPYSTSGSCAQSSKVDGWYVTNYVSPTFTLGDGYYAVIDTASFSYFGVDCDDLLVSPP
                     FDSHGATQLTIEFDTHFATYLSSIASVLLDADGALQVLWTKSVNVENQHITLGPISVA
                     GKKSLRVLWRYQGTYEYHWMVDNIKIQGK"
     gene            1338..3980
                     /locus_tag="PK710_18870"
     CDS             1338..3980
                     /locus_tag="PK710_18870"
                     /inference="COORDINATES: protein motif:HMM:NF019935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOT11848.1"
                     /translation="MSRELTVDERIKNVAPRLRIEVLDYLERHHHTLCSIVTSGRRTS
                     GIEACHFRMRVFDGLLDTLFHYASAMVAGPYASQRALGAVGSYARGSLMFHGGIDVRL
                     VIDSRDPDVDRFVRAMFRPLWDARVAIDVQVHHAESMIVLAENDWLGVASSLDWRHVA
                     GHEKMSTQLMKTVRDRVLSHDGFDSFLRRLESDGARRYQRFGHSAYQLEPHVKYGAGG
                     MRDLETVLWIAKARWNATGFTDMVRHGLFNPEEISELQLARSHLCRIHNLLHLLSGCR
                     SDRMSFDMQPRLASSLGYGDSAEAVERLMKEHYVHVNRVLRAKERIIDRARSAPSADS
                     IVERLPVGFCVNGDRIAISDVEALAENPILALTIFQFAIARQMRMDSDTLSLLRRALK
                     MRGFQKSVRSIPRSGDAFLSLCCSVQETRLPQDSPLSVMLDVGLLTTFIPEFKALAGR
                     RQRDAYHVLTVDAHCIAAVDLLRKLVRGEKQGSHSLFRRLAMEMARPRIVLLATLLHD
                     VGKAVAEKRHAMRGAPIAFEVAHRLGMSCDDAHEVAHLVRHHKLLNQIASRRDVDDPN
                     VWEEVAQVAHGREGLRELLLLSFVVISSTSPDAMTVWKRQWLERLFVRVDAHLRQRAD
                     FATRSAGVHSKIERIVMEQDNDETLRQYLRTMPERYILGQNEAKLVAHAHVAMNRKPQ
                     AVRVAVFTAFASSLAEVLVIADDRPGLLAVIAAVFMANQLSVLEAFIHSRKRSPTSVE
                     AVDMFHVVIPEGATIKALRQASFAMEKTISDILAGNTSSEQYVRGQKGAVHAFGRSEI
                     VVDNEASGSWTIVETFSEDRPGLLYTVANAFHDLRLNIQFAKINTEGTRAANVFYVSE
                     TDGSKVMDAARIDAIEQRLQEALR"
     gene            4001..4975
                     /locus_tag="PK710_18875"
     CDS             4001..4975
                     /locus_tag="PK710_18875"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT11849.1"
                     /translation="MHNVGKRWGWLGLVALFACGGPASQPAVPTGTTVQDPAGDEADA
                     EAMAEGEEAIKTKDFVKAKAVFSAIVARKPDHPKANHYLAVALENLGDAQGAEKHYRA
                     ALAAAPSWTDSVLNLSALLIDSQRFEEAEKVLASSASRNPKDAALQLNLAYARMGNKD
                     IEGAKTAFESALALADSPDVRLGLADLLVESGKYDEAKAHIDRAVQQAPENLEVLTMA
                     AELYRKARLPDDCIVAYDKAIALRAVAQLFSNRGVCKQMKKDNAGAKADYKKAIETDP
                     AYAPAHFLFGRFLLTVEKNKKAAIEAFEACEKIAPDSKCKQAAEEARR"
     gene            complement(5018..>5241)
                     /locus_tag="PK710_18880"
     CDS             complement(5018..>5241)
                     /locus_tag="PK710_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HOT11850.1"
                     /translation="ALVRQQRQGSDTHAPTTPPQANGSANIRDLEQRLTHRLGHKVQV
                     RDRGGKGEITIPYASLDELDEILEQLDVR"
BASE COUNT         1113 a         1432 c         1522 g         1174 t
ORIGIN      
        1 aagggcgtga agctcgagca cgaaagagct attcgcgcca agggatgagg cgcggtgtta
       61 cttcccttgt atttttatgt tgtccaccat ccaatggtac tcgtaggtcc cttgatagcg
      121 ccatagtacg cgcagtgatt tctttccggc caccgagatg ggtccaaggg tgatgtgttg
      181 gttttcgacg ttcacggact tggtccagag gacttgcagg gcaccatcgg catcgagaag
      241 aacggaggca atgctggaga ggtacgtggc aaaatgtgtg tcaaattcaa tagttagctg
      301 agttgccccg tgggaatcga acgggggaga gacaaggaga tcgtcgcagt ccacaccaaa
      361 ataggagaag gaagccgtgt cgatgacggc atagtagcca tcccctaagg tgaacgttgg
      421 ggatacatag ttggtcacgt accagccatc gactttcgag ctttgcgcgc aggagccgga
      481 ggtcgaatag gggccatccg ggctccagtt agggaaatag tcgaatgatt cttcgtgggt
      541 gaatccgcaa ggggggcaag gccctccaca atcgataccc gtttcccctt ggttttggat
      601 ttggtcggaa caacttgggc aaggggtagg gcaagggcct ccgcagtcta tgccttcctc
      661 cccttggttc tgaatttggt cgtcgcaaga tggggcagga gtgcctccac ctgaaccacc
      721 tgtcgttccg ccagttcctc caggaccgcc gccggtgcca ccagaggctc cgcctgaccc
      781 cgagctgcct gccgcgccgc cggttggcgc tgaaccgcca gcacctcccg cgccgccacc
      841 tcccgtgccg ccacctcccg tgccgccacc tcccgtgccg ccagcttgtc cggctacgcc
      901 agccgctcct cccccggctc ccgcaccccc ggtgcctgac tcgccagcgg aaccgccctg
      961 ttccccgcca tcggctcctg ctccgaaccc cgaggctccc gcggtattcg tccctcccgc
     1021 cgcccccggt gtccagctat ccggttccgt gtcttttcct gtcgcacagc cccaaatcaa
     1081 aaacacgctc aatgaggcta cgccaagcgc actcgagcaa cgcaagcgaa tctgacggtg
     1141 attgtacacg gtctcctcca tcaatggatc aagtcctagc acaaagacga tagccaaggt
     1201 tttccagaac gagttccagg tttggcggca ggacgttcgc gcaaccgacg atacgacatg
     1261 agcgaatgcg aatgatggat gcctactacc cctgtcctgt ggaatgcttg atagatgcac
     1321 gatgacagag agtcttcatg tctcgcgaat tgaccgttga tgaacgaatc aaaaatgtcg
     1381 cgccgcggct gcgcatcgag gtgctcgatt atctcgaacg gcatcatcac accctttgtt
     1441 cgatcgtgac atcgggacgc cgaaccagtg gtatcgaagc atgtcatttt cgtatgcgtg
     1501 ttttcgatgg gttgctcgat acgctttttc actatgcctc ggcgatggtg gctgggccct
     1561 acgcttcgca acgcgccctt ggtgccgtgg gcagctatgc tcgcggctct ttgatgttcc
     1621 atggtggaat cgatgtgcgg ttggtcattg attcgcgaga tcccgatgtg gatcgattcg
     1681 tgagagcgat gtttcgtccg ctgtgggacg cccgtgtggc catcgacgtg caggtgcatc
     1741 atgccgaatc gatgatcgtt ctcgcggaaa acgactggct tggcgtcgct tcttcccttg
     1801 attggcgaca tgtcgctggc catgagaaaa tgtccactca actcatgaaa acggtgcgag
     1861 accgcgtgtt gtcgcacgat ggtttcgata gctttctgcg tcgattggag tctgacgggg
     1921 ccagacgata ccagcgcttc ggtcattccg catatcaact ggagccacac gtgaagtatg
     1981 gcgctggcgg catgcgggat ctcgaaaccg tgctgtggat agccaaagct cgctggaatg
     2041 cgactggttt cacggacatg gtgcgccacg gccttttcaa cccagaagaa atctccgaac
     2101 tgcaactggc gcgatcgcac ctatgccgta ttcacaatct gctgcattta ctatccgggt
     2161 gcagaagtga tcggatgtcg ttcgacatgc aacctcggct tgcatcgagc ttgggatatg
     2221 gtgacagcgc ggaagccgta gagcgcttga tgaaagagca ttacgttcat gtcaatcgtg
     2281 tgttgcgtgc caaggaacga atcatcgatc gggcccgttc ggcgccatct gcggattcta
     2341 tcgtcgaaag actgcctgtc ggtttttgcg tcaacggcga tcggatcgcg atcagtgacg
     2401 tagaagctct ggcggaaaat ccaatccttg ccttgacgat ctttcaattc gccatcgctc
     2461 gacagatgcg catggattcg gacacccttt ctctgctgcg tcgtgcgtta aaaatgcgcg
     2521 gattccagaa gagtgtgcgt tccatccccc ggtctggcga cgcgtttttg agcctgtgtt
     2581 gttccgttca agaaacaaga ctgcctcaag actcgccgtt gagcgtcatg ctcgatgtcg
     2641 gcttgctcac cacgttcatt cctgagttca aagcactcgc gggccgtagg caacgagacg
     2701 cgtaccatgt ccttacggtg gatgcgcatt gtattgcggc cgtcgacctg ttgcgaaagc
     2761 tggtgcgcgg agaaaaacag gggtctcata gcctgtttcg acgtcttgcc atggagatgg
     2821 cgcggcctcg gattgtgctg cttgccacgc ttcttcacga cgttgggaag gccgttgccg
     2881 aaaaacgaca cgcgatgcgt ggagccccga tcgccttcga agtggcacat cggctcggca
     2941 tgtcgtgcga tgatgcgcat gaagttgccc atctcgtcag acaccataag ttgttgaacc
     3001 agatcgcttc gcgtcgtgat gtggatgatc ccaacgtatg ggaagaagtg gctcaagttg
     3061 cgcacgggcg agaaggtctg cgcgaactgc tgcttttgtc attcgtggtg attagctcca
     3121 cgtcgcccga cgccatgacg gtgtggaaaa ggcaatggct cgaacggttg ttcgttcgtg
     3181 tcgatgctca tttgcgccaa cgcgcggatt ttgccacccg aagcgccggg gttcattcga
     3241 agatcgaacg catcgtgatg gagcaggata acgatgaaac gcttcgacaa tatcttcgaa
     3301 cgatgcctga aagatatatt ttagggcaaa acgaggcgaa attggtagcg cacgcgcatg
     3361 tggcgatgaa tcgaaagccg caagccgtgc gtgttgccgt attcactgcc tttgcctctt
     3421 cacttgcgga agtgttggtc atcgccgacg atcggcctgg attgctggca gttatcgctg
     3481 ctgtattcat ggccaatcaa ttatccgtcc tcgaggcgtt cattcattct cgcaagcgat
     3541 cgccgacaag cgtggaagcc gtggacatgt ttcatgtcgt gattcccgaa ggagctacca
     3601 tcaaggcgtt acgccaggct tctttcgcaa tggaaaagac catcagcgac atcttggccg
     3661 ggaatacctc atcggagcag tacgtgcgtg ggcagaaggg agccgttcat gctttcggtc
     3721 gttcggaaat cgtggtggac aacgaagctt ccgggtcatg gacgatcgtg gaaacctttt
     3781 cggaagaccg cccgggtttg ttgtatacag ttgccaacgc ctttcatgat ttgcgattga
     3841 atattcagtt cgcaaaaatc aataccgagg gaacgagagc cgcaaacgtg ttttatgtgt
     3901 ccgaaaccga cggttcgaag gtgatggatg cggcgcgcat cgacgcgatc gagcaacgtc
     3961 tgcaagaggc tttgcgctga ccatgggaag ggagatcaac atgcataacg tcggaaagag
     4021 gtggggatgg ttggggctcg tggcgttgtt tgcttgtggc gggcctgctt cgcaacccgc
     4081 ggtgccaacg ggaacgacgg tgcaagatcc agcgggcgat gaggcggacg ccgaggcgat
     4141 ggcggaaggc gaggaagcca tcaaaacgaa agatttcgtg aaagccaaag ccgttttttc
     4201 ggcgatcgta gcgcgtaaac cagatcatcc caaggccaac cattacctag cggtggctct
     4261 cgaaaacctt ggcgatgcac aaggggcgga aaaacactat cgcgccgcgc ttgctgccgc
     4321 gccttcgtgg acggattccg tgttgaacct gtcggcgttg ctcatcgatt cccaacgctt
     4381 cgaggaagca gagaaagtgc tcgcctcttc ggcgtcgcgg aaccccaaag acgctgctct
     4441 tcagctcaat cttgcttatg cgcgcatggg caacaaagac attgaaggag cgaaaacggc
     4501 gtttgaatcc gctctggccc ttgccgattc gcccgatgtt cgtctgggat tggccgattt
     4561 gctcgtggaa agtggaaaat atgacgaggc gaaggcgcat atcgatcggg ccgttcagca
     4621 agcacccgaa aacttggaag tgctaaccat ggctgcggaa ttgtaccgaa aggcgcgcct
     4681 gccggatgac tgtatcgtcg cgtacgacaa agccatcgcc ttgcgtgccg tggctcagct
     4741 tttttcgaac cggggcgtct gcaaacaaat gaaaaaagac aacgcgggag ccaaagcgga
     4801 ttataaaaaa gccatcgaaa ccgatcccgc ctacgcgccc gcccactttc tttttggaag
     4861 atttttgctg actgtcgaaa aaaacaaaaa ggcggccatc gaagctttcg aagcctgcga
     4921 gaaaatcgcg cccgacagca aatgcaaaca agccgccgag gaggcacgcc ggtaaaacgt
     4981 tcgacctgca tcggtttcgc ccccgccgtc gcttacgcta tcgaacgtcg agctgttcga
     5041 gaatctcatc caattcatcg agggaagcgt aggggattgt gatttcccct ttgccaccgc
     5101 ggtcccggac ttgcacctta tgtcccaggc gatgtgtcaa ccgttgttcg agatcacgga
     5161 tgttggcgct gccattcgct tggggtggcg ttgtcggcgc atgggtatcg cttccttggc
     5221 gttgctggcg tacgagcgcc t
//
LOCUS       DAOSOR010000429         5235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15902_length_5235_cov_2.882819, whole genome shotgun sequence.
ACCESSION   DAOSOR010000429 DAOSOR010000000
VERSION     DAOSOR010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15902_length_5235_cov_2.882819"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PK710_18885"
     CDS             <1..318
                     /locus_tag="PK710_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HOT11851.1"
                     /translation="EANLALGFRDDERDYAVAAHMLHSLDVKSVRLMTNNPNKIRQLE
                     GYGVQVAGRIPHLLPPNPHNRFYLETKANRSGHYIDFGGKPHVNEQSDPVLVEGMQEP
                     TRH"
     gene            complement(386..1396)
                     /locus_tag="PK710_18890"
     CDS             complement(386..1396)
                     /locus_tag="PK710_18890"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOT11852.1"
                     /translation="MGSLRFGAEQAVRKCIRVQPNDTVVLVTDRECLEIGKEIENVVR
                     EITPSITTFIMEDFGERPDDGSRPLAFPQAIATALETATVSIYAASGKRGELKSFRSP
                     MLAVVDSKPNLRHGHMINITKQVMELGMSTDYDEIQALSAKVYDIVHKARRIRVTSPA
                     GTDFTVTLNPEWKWIISDGQITPAQWKNLPDGEVFTCAQSAEGKAVIDGCLGDYFSEL
                     GTCETFPVVVEMANGIVTRLTCEARPELEKELNDYIHQDENAKRVGEFAIGTNVGLDR
                     IIGNLLQDEKFPGVHIALGHGYPEKTGSPWASDAHIDLVMRRVTIEVEDQVIMRDGKF
                     LI"
     gene            complement(1716..2339)
                     /locus_tag="PK710_18895"
     CDS             complement(1716..2339)
                     /locus_tag="PK710_18895"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOT11853.1"
                     /translation="MFRPITVIVAALLLARPGIPQGEALRYATALQNEAKKHDFDPLT
                     GVALIWRESRWLPSAISSDREDYGLAQIRARHIGACRHDTDPVNHPSPECQAVKQALL
                     NGVYNIRVMGTLISRNRELCHDKIGTARFEQWLASYEGRNYPHRNQWCQPSDATREVI
                     RYRQWLLGKIEPPPRKPATARNSPKRGKQRVAAHPQAEKTKNNTRSP"
     gene            2486..3499
                     /locus_tag="PK710_18900"
     CDS             2486..3499
                     /locus_tag="PK710_18900"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT11854.1"
                     /translation="MKTTTAAWVAGLALVALGCGLVAGYDGVSFDRREGEADAGGAGA
                     MGGSGGVGGATGGTGAAAGAAGVGGATGGTGATAGAAGVGGATGGTGATGGAGGTSVV
                     CNSLEDLYGPLQICVAHSVQIDAWFIDVTEVTNKQYKAWLTYGPDPENDSTCKYAPEN
                     PKFGLEPKCDGDGGQYNGDDDYPVACIDWCAASAYCKGVGKHLCGSIDGGSVLSEDYN
                     KIAVSQWYRACTNGDADRYFPYPQAYDCQICNVTDEPSKVAQHGKCRTPLNIYDLSGN
                     VGEWEDCCDKEKCYIRGGYYSTYGMNDPCKQDGTLLRCDTENTIDKGAKHPFIGFRCC
                     TGP"
     gene            3786..4826
                     /locus_tag="PK710_18905"
     CDS             3786..4826
                     /locus_tag="PK710_18905"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT11855.1"
                     /translation="MKRSTKTWGVGLILLVAGCGWVAGYDGVSFDRDQQSYDAGQESG
                     GAEASAGMGGAAGGGATGGAAGETGGTAGETGGTAGETGGTGAASGAGGEAGVCGSCD
                     EYLEECHEKLRICVAKSIEIGINEKEKPWYIDVTEVTNKQYQDWLGTNPDIKAQVATC
                     VDKNNNFAPQCLPPVSRNEEPKYPVSCVDWCDAVAYCKAIGKRLCGSIDGDSTPWDEH
                     NNEKQDQWYAACVSGKERNWSFPYGNSFNCDKCNVKAGKASQVAEKDKCISYQNVYDL
                     SGNVAEWEDCCKENKLAKTRDCRIRGGHYYSINKFLCANSDGNAVKCDNKDDRDYLSR
                     QPNLGFRCCKGP"
     gene            complement(5042..>5235)
                     /locus_tag="PK710_18910"
     CDS             complement(5042..>5235)
                     /locus_tag="PK710_18910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11856.1"
                     /translation="MRGSALPTTMASHGRPGARLMQARDIRSSSMHAKSPHRNFSVPW
                     PFHRHELEYPMVHKLEYIK"
BASE COUNT         1229 a         1180 c         1615 g         1211 t
ORIGIN      
        1 gaagcgaatc ttgccttggg ttttcgggat gatgaacgag attatgccgt ggcggcccac
       61 atgctccaca gcctggacgt gaagtccgtg cgtctcatga ccaacaaccc aaacaaaatt
      121 cgccagctcg aaggttacgg cgtgcaggtt gccggacgca ttcctcatct gctgcccccc
      181 aacccacaca atcgatttta tcttgaaacc aaggcgaatc gttccggcca ttatatcgac
      241 ttcgggggca agccccacgt caacgagcag agtgaccctg tccttgtcga gggaatgcaa
      301 gaacccacca gacactgata cactcagaag cgtggcgtcg cacgcctcgt cgggctgcat
      361 caagaacagt gatttgaatg agatatcaga tcaggaattt gccatcgcgc atgatgacct
      421 ggtcttccac ttcgatggtg acgcgacgca tcaccaggtc gatgtgggcg tcggaagccc
      481 acggtgagcc tgttttttca gggtaaccgt gccccagggc gatatgaacg ccaggaaatt
      541 tttcgtcttg aagaaggtta ccgatgatac gatcgaggcc gacgttcgtg ccgatggcga
      601 actcaccgac gcgcttggcg ttttcgtctt ggtggatata gtcgttgagt tctttttcga
      661 gttccggtcg cgcttcacag gtcagtcggg taacgattcc gttcgccatt tcgacgacga
      721 cgggaaaggt ttcgcaggtt cccagctcgg agaagtagtc tcccaggcag ccatcgatga
      781 cggctttacc ttcggcgctt tgcgcgcatg tgaagacttc gccatcgggt aggtttttcc
      841 attgggcggg ggtgatttgc ccatccgaaa tgatccattt ccattcgggg ttgagggtta
      901 ccgtgaagtc ggtccccgcc ggagaggtga cgcggatacg tcgggctttg tgaacgatat
      961 cgtagacttt ggcggacagg gcttggatct cgtcgtagtc cgtggacatg ccgagttcca
     1021 tgacctgttt ggtgatgttg atcatatgac cgtgtcgaag gttgggtttg ctatcgacga
     1081 cggcgagcat gggggagcgg aaggacttga gttctccgcg ttttccggaa gcggcataga
     1141 tggaaaccgt agccgtttcg agagccgtcg cgatggcctg ggggaaggca agcggacggc
     1201 ttccatcgtc tggtcgttca ccgaagtcct ccatgatgaa ggtggtgatg gacggggtga
     1261 tttcgcgaac gacgttttcg atttcttttc cgatttcgag gcattcgcgg tccgtaacga
     1321 ggacgaccgt atcattgggt tgaacacgaa tgcatttccg tacagcttgt tcggcaccga
     1381 acctcaagga tcccatcgat cctcctttca accccccggg ttgagaacca gcacacggcc
     1441 tgatgcatcg gagaaggccg aggcaaacgt tccagcgtgt gggaagtagg ggcgagacag
     1501 tcgacgacga aggggctgcc agggccatcg tctttggtga atcgagctgt cgtacctacg
     1561 ctcacctaag ctgccgtagc gcaagcggcc aggcttttcc acccttgaaa cgcgaaggga
     1621 gcaacgaatt catcgtgatt atttgggaaa ggaacacgaa aggacgacaa aaatgcaata
     1681 tgcaggaagg acgccccctg gtgaggcaaa aaggatcaag gggatcgggt attatttttt
     1741 gttttttcgg cctgaggatg cgcggctacg cgttgtttac ccctttttgg ggaatttcgt
     1801 gcagtcgctg gttttcgtgg aggaggttcg atttttccga gaagccattg ccggtatcga
     1861 atgacttcac gggtggcatc ggaaggctgg caccattgat ttcgatgcgg gtaattgcgt
     1921 ccttcgtaac ttgcgagcca ttgttcgaag cgagcggtcc caattttatc atgacataat
     1981 tctcgatttc tagaaatcag ggttcccatg acgcggatat tataaactcc attcaaaagg
     2041 gcttgtttca cggcctgaca ttccgggctg ggatggttca cggggtcggt atcatggcgg
     2101 caggcgccga tatgccgggc gcgaatttgg gcaaggccgt aatcttcccg atcggaggag
     2161 atagcggagg gcagccagcg gctttcgcgc cagatgaggg ctacgcccgt gagcgggtca
     2221 aagtcgtgtt ttttcgcctc attttgcaga gccgtagcgt aacgaagggc ttcgccttgg
     2281 ggtatgccag gcctggcgag gagcagagcg gctacaatca cggtgatggg gcggaacatg
     2341 ggatgcaggc gttaccatga gtcggtctaa tgcaccaaga tcccttcctt gagatccctt
     2401 ccttgaatca cgccttccgg cacgctatcc tccgaagtcg atggtcccac aacgaacggg
     2461 accccaacga aacgggtcat tcgtcatgaa gacaacgacg gcggcgtggg ttgcgggttt
     2521 ggctctggtg gcgcttggct gtggcttggt tgcgggctac gatggggtca gtttcgaccg
     2581 tcgagaaggg gaagcggatg ccgggggggc gggagcgatg ggcggttccg gaggggttgg
     2641 tggagctaca gggggaacgg gtgctgccgc cggtgccgct ggggttggtg gagctacagg
     2701 gggaacgggg gcgacggcgg gtgccgctgg ggtcggtgga gctacagggg gaactggggc
     2761 aacagggggt gcggggggaa cgagtgttgt gtgcaatagt cttgaggact tatacgggcc
     2821 gttacagata tgcgtggccc actctgtgca gatagacgca tggttcatcg atgtcacgga
     2881 ggttacgaac aagcagtata aagcgtggct gacatatggc cccgatccgg aaaatgacag
     2941 tacatgcaaa tatgcaccag agaatccgaa gttcggtctc gaacctaaat gcgatggaga
     3001 tggaggacaa tataatggtg atgatgatta cccagtagca tgcattgatt ggtgtgccgc
     3061 ttcagcttat tgcaagggtg taggtaaaca tctttgtgga agtatcgatg gcggttctgt
     3121 actttcggaa gattataata aaatagctgt aagtcagtgg tatagagcct gtaccaatgg
     3181 cgatgcggat cggtattttc cctatccgca agcctatgat tgccaaattt gtaatgtcac
     3241 cgacgagcca tcaaaagttg cccagcatgg caaatgccgt acccctttga atatttatga
     3301 cttaagtgga aatgtcggtg aatgggagga ttgttgcgac aaagaaaaat gttatattcg
     3361 tggtgggtat tatagcacct atggcatgaa tgacccttgt aaacaagatg gcaccttgct
     3421 gaggtgcgat accgagaaca cgattgacaa gggggcaaag catcctttca ttggttttcg
     3481 atgctgcaca gggccgtgag cattgatgat caagaaaagc caatggcctg gaaagcgtca
     3541 gctatcgtcg ggcgaaaacg aaggggcgga gttgcgtatc tctattttct tatgaaatat
     3601 caataggata gctacagtcg gatcggtaac gcttacggta tcgagacggg acttcggggg
     3661 tgttggcctc gatgagctgg tccttcggaa ccaacggtct ccccttgaat tggggcttcc
     3721 gacgcggtat tctgagaagt catgctttgc aaatcggacg gatcctgggg aactgagtca
     3781 tccagatgaa aagatcgacg aagacatggg gtgtggggtt gattctgctt gtggcgggct
     3841 gcggttgggt tgcggggtac gatggggtca gcttcgatcg cgatcagcag tcctatgatg
     3901 ccgggcaaga gtcaggaggg gcggaagcat cggcgggcat gggaggggct gcgggtgggg
     3961 gtgcgacagg gggagctgcg ggagaaactg gcgggactgc tggggaaacc ggcgggactg
     4021 ctggggaaac cggcgggaca ggcgcggcaa gtggtgcggg aggagaggcg ggcgtctgcg
     4081 gaagttgtga tgagtatttg gaggagtgcc acgaaaaact aagaatatgt gttgcaaaat
     4141 caattgaaat tggcatcaat gaaaaagaaa agccctggta tatcgatgtc acggaagtga
     4201 cgaacaagca gtaccaagac tggctgggca ccaatcccga tataaaagct caggtcgcga
     4261 cgtgtgtgga taagaataat aatttcgctc ctcagtgtct gcctccggta tctcgtaacg
     4321 aagaacccaa atatcccgtg agctgtgtcg attggtgtga tgcggtagcc tattgtaagg
     4381 ctattggtaa acgtttgtgt gggagcatag atggagattc taccccttgg gacgaacaca
     4441 ataacgagaa gcaggaccaa tggtacgccg cgtgcgtatc tggaaaagaa cgaaactggt
     4501 cattccctta tggtaattcc ttcaactgcg acaaatgcaa tgtaaaagct ggcaaagcgt
     4561 ctcaggtagc cgaaaaagac aagtgcatat cgtatcagaa cgtatatgat ttgagtggaa
     4621 acgtcgcaga atgggaggat tgctgtaaag aaaacaaact ggctaaaacc cgagactgtc
     4681 gaatacgagg aggacattat tatagcataa ataagttttt atgtgctaat agtgatggca
     4741 atgcagtcaa atgtgataac aaggatgata gagattattt atctagacaa ccaaacctcg
     4801 gttttcgctg ttgcaagggc ccttgacgat tccttagaag cgcttcctgt cgcgcgaggc
     4861 atctgaccgg tggacgagac gaaaacaaca ctgcccgttt tcctctttca gtgaacccag
     4921 gaatcaggct tctgtatcgt ccgaacctcg ttgattgctt catgccgaaa agacgcaccc
     4981 atgagccatg gctcgccaag cgttggcctt cctggttgtc ccatgcaaac aactatttga
     5041 tttatttgat atattccaac ttgtggacca tgggatactc gagttcatga cgatgaaatg
     5101 gccatggtac gctgaaattt cggtgggggg acttggcatg catggaggag gatctaatat
     5161 cccgtgcttg catcaggcga gccccaggac gaccgtggga agccatggtc gtcgggaggg
     5221 cggaacccct catcg
//
LOCUS       DAOSOR010000430         5227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_15941_length_5227_cov_5.004447, whole genome shotgun sequence.
ACCESSION   DAOSOR010000430 DAOSOR010000000
VERSION     DAOSOR010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15941_length_5227_cov_5.004447"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="PK710_18915"
     CDS             <1..875
                     /locus_tag="PK710_18915"
                     /inference="COORDINATES: protein motif:HMM:NF024293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HOT11857.1"
                     /translation="GCQKRLAAEQQELAPEMGADDPGLEDPGESHRDSKVDRTIKCFN
                     GAMRINKAAQHYFGRLAGGLPRPGKVPSVVGVPPATTLQLCQEAKADLTPPMAEIDRI
                     MPRYVELVASLIRRLEQMDKYYKEKGYRGDGFRQGKQMHESFKSEQEEFQKLHDQLAS
                     AIDAVADKRDDEAIHKESKTRRLRYYSLVFLRDAKLLSREITRDKPDVPTITTMRLRV
                     ADSHKDLSTRAMSHPDEVDKAFMFAMYMGRADTFVQTVRNVDPTKLVDQDLDDMLNKY
                     NAMIDASNLVRWKP"
     gene            987..2273
                     /gene="tyrS"
                     /locus_tag="PK710_18920"
     CDS             987..2273
                     /gene="tyrS"
                     /locus_tag="PK710_18920"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOT11858.1"
                     /translation="MAECNVYDELVWRGLIDQATSPLIPDILKNEKVTCYIGFDPSAD
                     SLHVGHLLQILLLVRMQQAGHKPIALLGGATGVIGDPSGKSEERNLLDLDRVEHNLAG
                     IRAQLEHFLGTTETGAVTIVNNHDWLGSERLLGFLRDVGKHFSVNVMMSRDSVRSRLT
                     QREQGISFTEFSYMLLQAHDFLHLFDNYGCTFQLGGSDQLGNILGGIDLIRRCRKGQA
                     FGLTSPLLTTADGKKLGKTEKGAVWLDARKTSAYQFYQYWVRSDDRDVIKLLCMLTMR
                     SREEIEELTTQVEQAPHHRQAQQTLARDMTALVHGPQAAVEVERAAQALFGQAIAELN
                     ESTLLDVMSEAPSLELPATAFDHGGLELVDLLTRTQLCASKGAARKDISAGGIYVNNR
                     RIQDVGNRVCRSDALHGRYLVLRKGKKNFFLVKLMV"
     gene            complement(2296..4116)
                     /locus_tag="PK710_18925"
     CDS             complement(2296..4116)
                     /locus_tag="PK710_18925"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOT11859.1"
                     /translation="MSTDTNDINPAAASSAAPSPDDTTAAVSTETEAIQTHACDDDRS
                     QVEEPCSSEAVNPEQVTDEPCPGNEAPHVVAPAVAQTAWDDDWHEPGDDIGNRIDGPA
                     HHERTTTEVESASAKRRGKRSKKNDAKDDASNPRGKQNPKAKANAPTRERPAFPVGEE
                     VFGKVIEVLADAIFIDLSSKAKAIFDRHELSSDPPAAGDQFIATVHGDGARGGLVVLT
                     HNPRRFENAKDVVSEALSSGELVNALVTGVIKGGVELDIDGLRAFAPGSHVDLRLGAD
                     LSYLVGQRLPFKVMKYEKNGKEVVLSRRELLEKEATQARQDGLAKLQIGSVVEATVRS
                     VVEWGVFVAIPSANDIEGLIHISEASFERNAKLADLFKVGQSTEVKVLRVDDKGKLWL
                     SRKAVTGDPWEASASQFPVGSRVAGRVVRMQPFGVFVELVPGVDGLIRTADLSLLRIN
                     DPSEVISIGDEINVVVVSCDIKQRKIALHPALPGDEQEPRQKIAPYRTVQIEVVSADV
                     HGLTVRILGVTGASARGFIPAGHTNTARGTDLRVEYPAGTRLEAKIIDVDSKRGEVKL
                     SIRALKEDTEKAAYQEYRTKVARESKFGTLGDLLAKRRSE"
     gene            complement(4322..5119)
                     /locus_tag="PK710_18930"
     CDS             complement(4322..5119)
                     /locus_tag="PK710_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOT11860.1"
                     /translation="MIYGPELGRRIPLRRNSAMEIGRHPSNDFPLDQESVSRRHARIV
                     WSNDHWHAIDLGSTNGTYVNDELVTDRVLRDGDQIKVGRTILKFLEGGSVETSYHEEI
                     YRLMTFDGLTGAHNKRFFHEALEREVSRSKRYGRILSLILFDIDHFKKVNDTSGHIAG
                     DAVLRQLAALVRVNIRREDIFSRVGGEEFAIIVPETGVTEAAAVANKIRILVENAEIS
                     FDTITIPVTISLGVASLEANDTAEVLYAKADALLYRAKQMGRNRVVS"
BASE COUNT         1173 a         1415 c         1475 g         1164 t
ORIGIN      
        1 gggggtgcca aaaacggctt gcggccgagc aacaagagct agcccctgaa atgggcgccg
       61 acgaccccgg tttggaagac cccggcgaaa gccatcgaga ctccaaagtc gaccgaacga
      121 tcaagtgttt caatggcgcc atgcgaatca acaaagcggc gcagcattac ttcggccgct
      181 tggccggtgg attgccacga ccgggaaagg tgccctccgt ggtcggtgta cctcccgcca
      241 cgaccctaca actatgccaa gaagccaaag ccgatctcac gccaccgatg gccgagatag
      301 accgaatcat gccccgatac gtggagctgg ttgcctcatt gatccgtcga ctcgaacaga
      361 tggacaagta ctacaaggaa aaggggtaca gaggagacgg gtttcgacaa ggcaagcaga
      421 tgcacgaatc cttcaaatcg gagcaggaag agtttcagaa gcttcatgac caattggcca
      481 gcgccatcga tgcggttgcc gacaagcgtg acgacgaagc gattcacaaa gaatcgaaaa
      541 cgcgaaggct tcgatactac tcgttggtgt ttttgcgcga tgcaaaacta ttgtcgcgcg
      601 agatcactcg cgacaaaccc gacgtgccca cgataaccac gatgcgatta cgcgtggcgg
      661 acagccacaa ggacttgtcc actcgggcca tgtcccaccc cgatgaggtc gacaaagcat
      721 tcatgttcgc catgtacatg ggtcgcgcgg atacgtttgt gcagacggtt cgaaacgtgg
      781 atcccaccaa gctcgtcgat caggacctcg atgacatgct caacaagtat aacgccatga
      841 tcgacgcttc caatctcgtt cgttggaaac cgtgagccgg ttggtgccgc gggcatctcc
      901 ccctgcgatc gtgcgccacg gggcgctttg ccattgtatg gatagaccga ggcgtttagg
      961 aaggggacga aagccggagg accgtcatgg ccgaatgcaa tgtgtacgat gagcttgtgt
     1021 ggcgtggatt gatcgaccag gccacgagcc ctttgatccc ggatattctc aagaatgaga
     1081 aagtaacctg ttatatcggc ttcgatccga gcgcggactc ccttcacgtc ggccatctgc
     1141 tgcaaattct tttgctggtt cgaatgcagc aagcgggaca caaaccgatt gctcttcttg
     1201 gcggcgccac cggcgtgatt ggagacccca gtgggaaaag cgaagaaaga aacctcttgg
     1261 atctcgatcg agtcgagcac aacctcgcgg gtatccgagc acagctcgag cattttttgg
     1321 gaacgaccga aaccggtgcc gtcaccatcg tcaacaacca cgactggctt ggttcggagc
     1381 ggttgttggg attccttcgt gacgttggca aacacttctc ggtcaatgtg atgatgagcc
     1441 gagattccgt ccgttcacgg ctcacgcaac gcgagcaggg aatctccttt accgagttca
     1501 gctacatgct actccaagcc catgattttc ttcatctttt cgataattac ggatgcacct
     1561 tccaactcgg tggtagcgac cagctcggca acatccttgg tggtatcgac ttgattcgtc
     1621 gctgtagaaa aggacaagcc ttcggactca cctctccctt gctcaccacg gcggatggca
     1681 aaaagctggg caagacagaa aagggagcgg tctggctcga tgcacgaaaa acgagcgcct
     1741 atcagtttta tcaatattgg gtgcgctccg acgatcgaga cgtcatcaag ttgctttgca
     1801 tgctcacgat gcgaagtcgt gaagagatcg aagagctaac cacacaggtc gagcaagcgc
     1861 cgcaccatcg acaagcgcaa caaacactag cgcgagacat gactgccctg gttcatggac
     1921 cgcaggcggc cgtggaggtc gagcgtgctg cgcaggcttt gttcggacaa gcgattgcgg
     1981 aattgaacga atccactttg ttggatgtta tgtccgaagc cccatcgttg gaactacctg
     2041 ccaccgcttt cgatcacggg ggtttggagc tggtcgatct tcttacgcgc acgcaattat
     2101 gcgcgtccaa aggagccgct cgcaaagaca tcagcgcggg gggaatctat gtgaacaatc
     2161 gtcgaatcca agatgtgggc aacagggtct gtcgatccga cgctttgcat ggtcggtacc
     2221 tcgtgttgcg caaaggcaaa aagaattttt ttctggtcaa attgatggtg tgagcgtgtg
     2281 caatccgaag cgagatcatt cgcttcgacg ttttgccaat aggtctccga gcgtgccgaa
     2341 cttggattcg cgcgctacct tcgttctata ttcttgatag gcggctttct cggtatcttc
     2401 tttgagtgcg cgaatcgata gtttgacctc gcctcgcttc gagtcgacat cgataatctt
     2461 ggcttcaagt ctcgtacccg ctgggtactc cacgcgaaga tcggttccac gagcggtatt
     2521 ggtgtgaccc gcggggatga agccccgggc actcgcaccc gtcacaccaa ggatacgaac
     2581 cgtcaaaccg tgaacgtccg cggaaacgac ttcgatctgg accgtgcgat agggagcaat
     2641 tttttgacgc ggttcctgtt cgtctccagg caatgcaggg tgcaacgcga tttttcgctg
     2701 tttgatatcg cagctaacga ccaccacatt gatttcgtcc ccgatcgaaa tgacttcaga
     2761 cgggtcattg attcgcagca gggaaaggtc ggcggttctg atcaaaccat caacccctgg
     2821 gacgagttcc acgaaaacac cgaaaggttg catgcgcacg acccgtccag ccacccgaga
     2881 tcccacggga aactgagatg cgctggcttc ccatggatct ccggtaaccg cttttctgct
     2941 gagccaaagc ttgcctttgt catcgacacg aagaactttg acttcggtgc tttgtccaac
     3001 tttgaacaag tcggcgagtt tggcgttgcg ttcgaaggaa gcttccgaaa tgtggatgag
     3061 tccttcgata tcgttggcgg aagggatggc gacaaatacc ccccattcga cgacggatcg
     3121 cacggtagct tcgacgacgg agccaatttg aagtttggcg agcccgtctt gacgggcttg
     3181 ggtagcttct ttttcgagca attcacgacg agacaggacg acttccttgc cgtttttctc
     3241 gtacttcatc accttgaagg gcaatcgttg ccccacgaga taagacaggt cggctccgag
     3301 acgaaggtcg acatgggaac caggagcgaa agcgcgaagt ccatcgatgt cgagttccac
     3361 gccgcccttg atgacgcccg tgacgagtgc attgacgagt tcaccggaag agagtgcctc
     3421 ggagacgacg tctttggcat tctcgaagcg tcggggattg tgggtaagca cgacgagtcc
     3481 gcctcgcgct ccgtcgccat gcaccgtggc aatgaattgg tcacctgccg cgggaggatc
     3541 ggaggagagt tcgtgacggt cgaatatcgc ttttgccttg ctggacaggt caatgaagat
     3601 ggcatcggcg agaacttcga tcacttttcc gaacacttcc tcgccgacgg gaaaggcagg
     3661 gcgttcacgg gtgggtgcgt ttgctttggc tttcggattt tgtttgccac ggggattcga
     3721 agcatcgtct ttcgcatcgt tctttttcga acgcttgcca cgtcgcttcg ccgatgcgga
     3781 ctcgacttcg gtggtcgtgc gttcatgatg agcaggccca tcgatacgat taccgatgtc
     3841 gtcaccgggc tcgtgccaat cgtcatccca cgcggtttgc gcgactgcgg gagcgacgac
     3901 atgcggtgct tcatttcccg ggcaaggctc gtcggtgacc tgctcggggt tcaccgcctc
     3961 gctggagcac ggctcttcga cctgcgatcg gtcgtcgtcg cacgcgtggg tttggatagc
     4021 ttcggtttca gtggatacgg cggctgtcgt atcgtctggt gagggagcgg cagaggatgc
     4081 ggccgctggg ttgatgtcgt tggtgtcggt gctcatcgca cgcctcgata ggggatgaaa
     4141 gagggcgcag aggatagcgt atgggtctgc gacctgcgac aaggatcgtg tgactgttca
     4201 aagatgaaag aaggtgcata ccacgagatg gccagccatg ggttggtaag cacaacagaa
     4261 aacacgacag cccccatgca tcgactctcc ggatagtgca aggtggtatc ggcaggggac
     4321 gtcaggaaac gacgcggttt cgtcccattt gtttggcacg atacaaaagt gcatcggctt
     4381 tggcgtacag cacttccgcg gtatcgttgg cttccagact cgccacaccc aggcttatgg
     4441 tgaccgggat tgtgatggtg tcgaacgata tttcggcgtt ttccacgaga attctaattt
     4501 tgttggccac cgccgcggcc tccgtcactc cggtttcagg cacaataatt gcgaattcct
     4561 cccctcccac ccgcgaaaat atgtcttccc gtcggatatt gacccggacc aaagccgcga
     4621 gctggcgcaa aaccgcatcg cccgcaatat gaccagaagt gtcattgacc ttcttgaaat
     4681 ggtcgatatc gaacaaaatc agtgacaaaa ttcgtccata tcgtttgctt cgggacactt
     4741 cccgctccaa ggcttcgtga aaaaagcgtt tgttgtgggc gcccgtaaga ccatcgaacg
     4801 tcatcaaccg atagatttcc tcgtgatacg aggtttccac gctaccgcct tcgagaaact
     4861 tcaagatcgt ccgaccgacc ttgatctgat caccatctcg caatacccga tccgtcacga
     4921 gttcatcatt gacataagta ccgtttgtcg acccaaggtc gatggcatgc caatggtcat
     4981 tggaccagac gatacgggcg tggcgacgac tgaccgattc ctgatcgaga ggaaaatcat
     5041 tcgagggatg ccgtccgatt tccatcgcgc tgtttctgcg caaaggaatt cgacgtccga
     5101 gttcaggtcc gtagatcatc acgagacatg cttccccgga cggcgcagac gcattgttcg
     5161 gcgttggttt cagtacggtg atgaccgttc tttcgtcatc ggctggacgg cgaggcacgc
     5221 cttagga
//
LOCUS       DAOSOR010000431         5196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_16078_length_5196_cov_4.623614, whole genome shotgun sequence.
ACCESSION   DAOSOR010000431 DAOSOR010000000
VERSION     DAOSOR010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16078_length_5196_cov_4.623614"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1026)
                     /locus_tag="PK710_18935"
     CDS             complement(112..1026)
                     /locus_tag="PK710_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11861.1"
                     /translation="MRSSFQLRIALLVVAVVLLGGRVSLADELSEFEKGRNSYSVGRY
                     EEAAERFAQMLDSNSDDPLTSPQLIEQARIYRAASLLAIGRLAEADHEIETILRTNPS
                     AYPDPVVFPGIVLDRFTDVRGRIRQELESKAQEKARQEQLRLQREHERRERERERVAA
                     LEKIAQQETHTEHHSRWLAALPFGVGQFQNDQTALAWTLLVTETALATTTVVTSAVVQ
                     NLQSKGTEPNVDPIALNERVSTTKTINHVAFAAWVLTAAGGIAHAQWTFVPERKHVRK
                     RELPPELRPAPTIGVVPGGALLEWRAQF"
     gene            complement(1064..2851)
                     /locus_tag="PK710_18940"
     CDS             complement(1064..2851)
                     /locus_tag="PK710_18940"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT11862.1"
                     /translation="MSIVPSAAPELVKYRLLDEIGHGGMATVYRAIDLRLDREVAVKL
                     MHPHLRENREAAARFVTEARAVAQLRHQGIVEIYDVSDENDDERFLVAQLVRGPSLRA
                     LLREHAEVPAEIGACFGVLLCDALGHAHDKGIIHRDVKPENVLVELDDSRKIAVVKLT
                     DFGIAKVIDAQGVTSTGQVLGSPAHMAPEQIEGGGVDVRTDIFGLGVLVYECMVGHLP
                     FEGKNPAQVLRKVLDGNFPALDRERPTIGSRWAHIVAKAIAKEPSQRWDDIRTFGESM
                     RQQLEQVGIRDPRAEIYAYFSAPDSYRASFVPRLVENLVARADRARQEGDTAGAAADL
                     NRAVAYSPDDQELLGQLTQLATGVRRQRWLARAKRGALLALIGAVATVLLMVLSKPPP
                     LGIPEKPYLPAHTASIRLRPTASPTASPVAVVSSFKLPAIRASVRPILSSVPKAERAV
                     TIVASPGSALVSIDGSAPIKVGFGITQNLLIGRHTLVFSTPSGDPCCLPRTVHVDVVA
                     GEGAQVVNGTVQYRDARLSLVGGPADALLDCPGIGKSVKTGETVTIGMATVDQFVNCF
                     ISGSGIPPGAKSIKLRAGQSVAVSALDPR"
     gene            complement(3225..>5196)
                     /locus_tag="PK710_18945"
     CDS             complement(3225..>5196)
                     /locus_tag="PK710_18945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11863.1"
                     /translation="LGTAPAQLLRRLVEAGGVLQVRVVPPTLEPLAARGLVFARMHES
                     NCIELVLPPAYLVQLPMWEGEDPRGIRALLAQSSAETQAAIASHYAGRPATHPIALPL
                     EEAWSVLSNPEALAREIATLSSTERRLLDSVYQEGCEVDTEELLDLEREPLRLRNATG
                     AAPSRRGVSFSLERRGMLIPVHPNRHIIPTEVAAIIGAEDVSSRKSKRAQIRAFVLDG
                     DHEPRRARFALDPSPIAIALAMAAREGGTEVRETAGTPRSLLLRLSQRFGRDFQTVAL
                     LVALSRALGLWEGSSLSRATPPGAWSLSELGLALFRVWRQGGAWDEGRPEPEVLRLPP
                     DARDSSPVRIVREIVLDALEDLAEGRWLPFEAIADWVRSDPRTPGVTRLLRRWALRVG
                     LEPPLPTDIAQTIVLESLPALGILDVGEADTDHDVVDAPPLVRITPRGRAYFQGNVPQ
                     AESSSTAFMQETVLRIGSEAAVGSVLTLYPFCEVGKVDDTLEVEITNAAISRALASGV
                     DGEVIRATISALVDPSITVAKALDQLSVVVGRITFVAASGFLWCDDPDVREMLRSRKQ
                     TAECFIDPSPPAGLLLSPTVSLDALARRCRVLGVEVISEGQVVRARSTIPPPMLDNSP
                     ARPSGARPTPRTDNPVRSSRSRKPSKSPSRPP"
BASE COUNT         1163 a         1723 c         1305 g         1005 t
ORIGIN      
        1 gggagcccac tgcatcaacg tcaatctcga accctcggga aacccttcct ttcatgagga
       61 attgatcggt cctgcggaac aaatcctgcc acgtctcttc ggagtagaag tctaaaactg
      121 ggcccgccac tccagcaacg ccccaccagg aacgacccca atcgttggcg caggcctcaa
      181 ctccggagga agctcccgct tgcgaacgtg cttgcgctcc ggaacgaacg tccattgcgc
      241 atgcgcaata cctcccgccg ccgtcaatac ccacgccgca aaagccacgt gattgatcgt
      301 tttcgtcgtg gatactcgct cgttcaaggc aatcggatcc acattgggct ccgtgccctt
      361 ggactgaaga ttctgcacaa ccgcactcgt gactaccgtg gtggtggcca acgccgtctc
      421 cgtaaccaat aacgtccacg caagcgccgt ctgatcgttt tgaaattgcc ccacaccaaa
      481 aggaagcgcc gccaaccacc gagaatgatg ctccgtatgc gtttcttgtt gcgcgatctt
      541 ttcgagagcc gctacccgtt cgcgttcccg ctcccgtcgc tcgtgctctc gttgcaatcg
      601 aagctgctcc tgacgcgctt tttcttgcgc tttgctctcc aattcctgac gaatccgtcc
      661 tcgaacatcg gtaaatcgat cgaggacgat tccgggaaat actaccgggt caggataagc
      721 actgggattg gtacgcagaa tggtttcgat ttcatgatcg gcttccgcaa gacgaccgat
      781 ggccaacagg ctagccgctc gatagattcg cgcttgctcg atgagctgtg gcgaagtgag
      841 aggatcgtcg ctgttgctgt cgagcatctg cgcaaaacgt tcggccgctt cctcatacct
      901 tcccacgctg taggagttgc gtcctttctc aaactccgat agctcgtcag caagcgacac
      961 tcgtccccct agcagcacca cggccaccac gagcaacgcg attcgaagct ggaaagacga
     1021 gcgcatgcgt aagctgactg tctttgccac ggtagcccaa tgcttaccga ggatcgagtg
     1081 cggagaccgc caccgactga ccagctcgta gcttaatgga ctttgctcca ggagggatcc
     1141 ccgaacctga aatgaaacaa ttcacaaact gatccaccgt cgccatcccg atcgtgaccg
     1201 tttcccccgt cttgacgctt ttgccaattc ctgggcagtc cagtagcgca tccgcgggcc
     1261 cccccaccaa ggaaagacgc gcatcccggt attgtacggt cccgttcacc acttgagcac
     1321 cctcacccgc tacaacatcc acgtgtacgg ttctcggtaa acaacacgga tcccccgagg
     1381 gcgtgctgaa taccagcgtg tgccgcccaa ttagcaagtt ttgggtgatt ccaaaaccca
     1441 ctttgatcgg cgcgctgcca tcgatgctga ccaatgcact gcccggcgaa gccacgatgg
     1501 tcacagcccg ttccgccttg ggaaccgacg aaaggatcgg tcgtacactt gcgcggatcg
     1561 ccggaagctt gaacgacgac accaccgcaa ccggagatgc cgtcggagaa gccgtgggac
     1621 gaagacgaat ggaggcggta tgagccggta aatatggctt ttctgggata cccagcggcg
     1681 gtggcttgct taggaccatc aacaataccg tcgctaccgc cccaatcaac gccaacaatg
     1741 cgccacgctt cgccctggcc aaccaacgtt gacgccttac cccagtcgcc aattgcgtca
     1801 gttgaccaag caactcctga tcgtcggggg agtaggccac cgcacgattc aaatccgccg
     1861 cggcccccgc cgtgtctccc tcctgccttg ctcgatccgc tcgtgctacg aggttttcca
     1921 caagacgcgg tacgaaagag gcgcggtatg agtcaggtgc ggaaaaatat gcatagattt
     1981 ccgctcgggg atctcgaatc cccacctgct cgagctgctg acgcatcgac tcgccaaacg
     2041 ttcgaatgtc atcccatcgc tgcgatggtt ccttggcgat cgctttcgcg acgatatgag
     2101 cccaccgact gccaatcgtg ggccgctccc ggtccagcgc gggaaaattc ccatccaata
     2161 ccttgcgaag cacctgcgcc ggattcttcc cctcgaaagg aagatgcccc accatgcatt
     2221 catagaccaa tacaccaagc ccaaaaatat ccgtcctcac atccacccca ccaccctcga
     2281 tctgctcggg cgccatgtga gccggagacc ccaatacttg acccgtcgat gtcaccccct
     2341 gcgcatcgat gactttcgct atgccaaaat ccgtgagctt gacgaccgcg atcttccgcg
     2401 agtcgtccaa ctccaccaat acattctccg gtttgacatc ccggtgaatg atccccttgt
     2461 catgcgcatg cccaagcgca tcgcaaagca ataccccaaa acatgccccg atctccgcgg
     2521 gtacctccgc atgctcgcga agaagcgcac gcaacgatgg cccccgcacc aattgcgcca
     2581 cgagaaatcg ctcgtcgtcg ttttcatcag atacatcgta tatctcaacg atcccctgat
     2641 gacgaagctg cgcgaccgct cgcgcctccg tcacaaaccg cgccgcagct tctcgattct
     2701 cccgcaaatg cggatgcatc agcttgaccg ctacctcccg atcaagccga agatcgatcg
     2761 cccgatatac cgtcgccatg ccaccatgac caatctcatc cagcaatcgg tacttcacca
     2821 actccggagc cgccgacggt acaatactca tcgcctctcc ttatcagcgg cgacgcacac
     2881 tttcgaacgg gtcctccggt gtcgattgga atcggcctgg gcttccatga cgatgcttgg
     2941 gcaggatgcc acatccatgg cccgtgtggt tcgagaacga gccgcaatct cacaccactg
     3001 gctcgaagat tcaatggccc gaacaccaca ggcaatgcct tcgaatctcc gcgataataa
     3061 atatatttgt aatatcaata ggttacagtt gtttggacaa gacccccgca cccgctcaac
     3121 gctcctttga tgatttctcc gccgcggtgc ggccaagccg tcaatggtca ttgatggcta
     3181 gccgtcggtt gactcgccct cctcgctcgt gcgccttacc ccccttacgg tggtcgagaa
     3241 ggagatttcg aaggcttacg agacctcgac gaacgaacag gattgtccgt ccgtggtgtc
     3301 ggccgcgcac cacttgggcg tgcaggagaa ttgtccaaca tcggaggcgg aatcgtcgag
     3361 cgcgcacgca cgacctgccc ttccgaaatc acctcgaccc caagaactcg acaacgcctt
     3421 gccaaggcat cgagagaaac cgtcgggctt agcaataatc ccgcaggggg cgatggatcg
     3481 atgaaacact cggccgtctg ttttcgtgag cgaagcatct cgcgcacgtc cggatcgtcg
     3541 caccataaaa agccagaagc tgccacgaat gtgattcgtc ccacaacgac actgagctgg
     3601 tcgagggctt ttgcgaccgt gatgcttggg tcgaccaaag cggaaatggt ggcccgaatg
     3661 acttcaccat cgacaccgga agccaacgct ctcgaaatgg cggcgttggt gatctcgact
     3721 tccagcgtat catccacctt gcccacctca cagaacggat agagcgtcaa taccgaaccc
     3781 acggctgcct cggagccaat acgaagcacc gtctcctgca tgaacgcagt gctcgacgac
     3841 tctgcttgcg gcacgtttcc ctgaaagtag gctctacccc gtggtgtgat gcgcaccaac
     3901 ggcggtgcat ccaccacatc atggtccgta tcggcttccc ccacatccaa aatgcccaac
     3961 gcgggaaggg attcgagcac gattgtttgc gcgatatccg tgggcaaagg cggttccaag
     4021 ccgacacgca acgcccagcg tcgaagcagc cgagttaccc ccggtgtccg cggatcgctg
     4081 cgaacccaat ccgcgatcgc ttcgaaggga agccagcgcc cctccgccaa gtcctccaat
     4141 gcatcgagta cgatttctcg aacgatgcgc acaggactgg agtcccgagc gtcgggaggc
     4201 aaccgaagaa cttccggttc cggtcgaccc tcatcccaag ctcctccctg acgccatacc
     4261 cgaaacaatg ccaagcccaa ctccgataaa ctccaggctc cgggaggggt cgcccgcgac
     4321 aacgaagacc cctcccacag tccaagggca cggctcaacg cgacaagcaa cgccacggtt
     4381 tggaaatcac ggccgaatcg ttgggaaagg cgaaggagca aagaacgcgg agtccccgcg
     4441 gtttctcgaa cttccgtgcc cccttcgcgc gcagccatcg ctagcgcaat ggcgatggga
     4501 gacggatcca aagcaaatcg cgcccggcgt ggctcatgat ccccatcgag cacgaacgca
     4561 cgtatctgcg cacgcttgct tttcctcgaa gacacatctt ccgcaccaat gatggcagcg
     4621 acctccgtcg gaatgatgtg acggttggga tgcaccggaa tcaacatccc cctgcgctcg
     4681 agagagaaac ttacaccacg ccgggaaggg gccgctcccg ttgcgtttcg caatcgcaag
     4741 ggctccctct ccaaatcgag taattcttcc gtatcgacct cacatccctc ctgatacacg
     4801 gaatcgagca atcgacgttc cgtggacgat agcgtggcga tttctcgcgc caatgcttca
     4861 ggattcgaca gcacgctcca cgcctcttcc agaggcaaag caatcggatg agtcgcggga
     4921 cgtccggcat aatgggaagc aatcgccgct tgcgtctccg cgctgctttg cgcaagcaac
     4981 gcgcgaattc ctctcggatc ctccccctcc cacatcggca gttgcaccaa ataggccggc
     5041 ggaagaacca actcgataca attgctttca tgcattcgag caaataccaa cccccgagcc
     5101 gctaggggct cgagcgtcgg tggcaccacg cgaacttgca gcacgccccc tgcttccacc
     5161 aaacgacgta acagttgcgc tggagccgtt cccaat
//
LOCUS       DAOSOR010000432         5190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_16113_length_5190_cov_3.780136, whole genome shotgun sequence.
ACCESSION   DAOSOR010000432 DAOSOR010000000
VERSION     DAOSOR010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16113_length_5190_cov_3.780136"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..295
                     /locus_tag="PK710_18950"
     CDS             134..295
                     /locus_tag="PK710_18950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11864.1"
                     /translation="MGCSSAACGTAIGALIGGPPGAGIGYLIGLGLGSAGGAVAGVKA
                     VTATKKLMQ"
     gene            329..478
                     /locus_tag="PK710_18955"
     CDS             329..478
                     /locus_tag="PK710_18955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11865.1"
                     /translation="MHPELVKKAFAVGLTEALKLFAAIPTKELASAVAKSALTVIKKL
                     KTVAK"
     gene            564..2363
                     /locus_tag="PK710_18960"
     CDS             564..2363
                     /locus_tag="PK710_18960"
                     /inference="COORDINATES: protein motif:HMM:NF021006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM1812 family CRISPR-associated protein"
                     /protein_id="HOT11866.1"
                     /translation="MGRDVLFSFLGTNDYVPVRYSLRGAPTSTRETFVQCALVRSLKA
                     ENRPVDLAILAMTKEAKDKHSTAIRDQLQSLELLVQALDIPTGNSEEELWEIFKTIGT
                     AFQRDDRVVFDLTHGFRSLPSTGLLAMSYFRHVLGIDVQVYYGAFEVLGSFHDVKQRH
                     KDNDEAFFQENIAPIFDLTPMFVLPAWAEAVAEWKRTGRASGLVQQSQPHTAALQRAL
                     KREAPRALTGLPQDLKMLSDALMIMRHDSIGARANEVIEHAEEAKEHIEKEPRLAPLT
                     YVLDQVRDSVEPLRGNGGAWQDYPDSYLRHQLQIVRWLSEHNRIVEAFSFLRELVISC
                     AVRILKNAGIEEIDGFKPSDVDFREKAGGICAYAIGAKSDIKEKEIGTQVKAWLQSHP
                     SILENFRDASQITQNNRNRLDHCWMGEEHGRQRFTKSMAETISKDLERAITAADRLLH
                     CLSNEISSNYNELQDIGPGASALQPCFINLSNHPIDTWSEAQRQGALELGLGTPVELA
                     GGMPLVPPEAGPDDIAKLAEDIVGRAMKQGVRGAFVAGEFTLTYALIQALCRQNIRCF
                     TATTKRQASEVVAPDGSVTRSSVFAFVRWREYV"
     gene            2532..3386
                     /gene="cas6"
                     /locus_tag="PK710_18965"
     CDS             2532..3386
                     /gene="cas6"
                     /locus_tag="PK710_18965"
                     /inference="COORDINATES: protein motif:HMM:NF021551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR system precrRNA processing
                     endoribonuclease RAMP protein Cas6"
                     /protein_id="HOT11867.1"
                     /translation="MTGFELHAWRIRIRALENLELPCLPTVALRSVTGMALREMSCVR
                     RERTSCGGCPEQEDCVYSRLVEPAGKGQSPGVTDVAPPPLVMAPEARTLDDAPIRLSK
                     GATIDVRAVFMGGARVHASLWLTALRSGLARGVGKERRALEVVDAQPLDVTFCAAGKK
                     AEIELWTPLRLKSQGKIRSRFEAGDFVQGLMHRVTLLSETYRLTRPAGFSEDSVRLKH
                     CDGRVIHVRRWSGRQKASMNLPGLTGTLVLEGELEGLWPWLALGEWVQMGKGTSMGFG
                     RYRLRAIG"
     gene            3397..>5190
                     /gene="cas10"
                     /locus_tag="PK710_18970"
     CDS             3397..>5190
                     /gene="cas10"
                     /locus_tag="PK710_18970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR-associated protein Cas10/Cmr2"
                     /protein_id="HOT11868.1"
                     /translation="MNQRERDARFWQAKVAAWVHDPAEKALVLLRDPQGHEGGTSKKL
                     REELFPQGLDESLKKLVKEADHWAAAADRPQFPRSAHDGRYASWSQVDFVSEPQLIHP
                     LSGDSVSLQDFEDIDKEHIKAVSFDHLSDLIHNENGAIDYRRTMLALWRFGSESPARG
                     IGALWSMLPADTRTPDHSIWEHLRLCSAYAGATCDGQASLLLLSLGPVQGFIAQARST
                     SDLWAGSHLLSRLSWEAMRVVAEWCGPDAILFPNLHGVPIVDLWLVEQGLDFSRSKGI
                     FPDWMRFASDANPLFIAALPNRFLALVPESIAEDLAREVRTRVRDKAKSLACEAWRCV
                     ATLAELDEGDGLSQIADQLHGFPEVHWAVIPASLAKSSSDLQKAMEPFFPADDTPPGF
                     LGSSLFRALSKDTTLEGTVFFPPGPGTLYPALYDLTERTHAAAKTSQTFSALNQEGYR
                     CSLCGEREWLTTDRKLLAYHTQDNQPGSPWPIVAQNPRAWAKPGERLCALCCTKRLWP
                     TLFSEELKTILEKTPETVDIPLKSTVISGQIQRYVVSTHTMALASTFREMARGFIKNN
                     NKLKELAGHLEEYRHTALPRQLAHASVSDDLV"
BASE COUNT         1118 a         1389 c         1585 g         1098 t
ORIGIN      
        1 ccccccgtcg gcgtctgtcc aagtatgagc atgaaaaaaa accacaaccc tagccgcgac
       61 attcaccagc aaagcgttgg aactggttcc aagcgctgaa attaccatcg ggatccccct
      121 tgggatggtg acggtgggat gcagctccgc ggcgtgcggc accgcgattg gcgcgttgat
      181 cggcggtccc ccgggcgctg gcatcggcta tctcatcggg cttggcttgg gaagcgccgg
      241 aggtgccgtg gcaggagtca aagcggtaac cgctaccaaa aaattgatgc aatagaaatc
      301 gggttcttga acccaaagga ggtatgacat gcaccctgaa ttggtaaaaa aagcatttgc
      361 cgttggcttg acggaagctc tgaaactttt cgccgctata cccaccaaag aactggcgtc
      421 ggccgtcgcg aaatccgccc tcaccgttat caaaaaactg aaaaccgtag cgaaataggc
      481 aaaggggtta cacaactccg gaggcccttc cacgctgccc acaagccgtg ttagcattct
      541 ggcgtgagaa aggcgggttc atcatgggaa gagacgtatt gttcagtttt cttggcacga
      601 atgattatgt tcccgtgcgc tactcactgc gcggagcgcc cacatccacg cgagaaacct
      661 tcgtccaatg cgcgcttgtc cggagcctga aagcggaaaa ccggcctgtg gatctggcca
      721 tccttgcgat gacgaaagag gcgaaggaca agcacagcac agccatccga gaccagctcc
      781 agtccctgga gcttcttgtc caagcccttg atattcctac aggaaattcg gaagaggaac
      841 tctgggagat cttcaaaacc atcggcaccg ctttccagcg agacgaccgg gtggtattcg
      901 accttaccca tggtttccgt tcgcttccat ccacggggct gctggccatg agctactttc
      961 gccacgtttt ggggatcgac gtacaggtat attacggcgc gttcgaggtg ctagggtcgt
     1021 tccacgacgt aaagcaaagg cataaagaca acgatgaggc gttttttcaa gagaatattg
     1081 cccccatttt cgatctcacg cccatgttcg tcttgcccgc gtgggcggaa gccgttgccg
     1141 aatggaaaag gacgggacgg gcatctgggc tcgtgcaaca gagccagccg catacagccg
     1201 cgctgcaacg agccctgaaa agggaggcgc ctcgggctct gaccggattg cctcaagacc
     1261 tgaaaatgct ctcggatgcg ctgatgatca tgcgtcatga cagcatcggc gcccgggcca
     1321 acgaggtgat cgagcatgcg gaggaagcca aagaacacat cgaaaaagag ccgcggctcg
     1381 ctcccctcac ctacgtcctc gaccaggtgc gcgatagcgt cgagccgctg cggggcaacg
     1441 ggggagcatg gcaggactat ccggattcct accttcggca tcaactgcaa atcgtgcgct
     1501 ggctgagcga gcacaacaga atcgtcgaag cgttctcgtt tttgcgagaa ctagtgatct
     1561 cgtgcgcagt acggatcttg aaaaatgctg gaatcgaaga gatcgacggc ttcaagccct
     1621 cggacgtgga tttccgtgag aaagccggag ggatatgcgc ctacgccata ggagccaaaa
     1681 gcgacatcaa agaaaaggag atagggacgc aagtgaaggc gtggctccaa tcccacccaa
     1741 gcatcctcga gaatttccgt gacgcgtcgc aaatcacaca aaacaaccgt aaccgactcg
     1801 accattgttg gatgggagag gaacatggca ggcagcgttt caccaagtcc atggcagaga
     1861 ctatctcgaa ggatctggaa cgtgcgatca cggccgcgga caggttgttg cactgtctgt
     1921 ccaatgaaat atctagcaat tacaatgagt tacaagatat cggaccaggc gcttctgccc
     1981 ttcaaccctg cttcatcaac ttatcgaacc atcccatcga cacctggagt gaagcccagc
     2041 ggcaaggggc tttggagctt ggattgggaa cccccgtgga attggctggg ggaatgcccc
     2101 tggtgccgcc ggaagcgggt ccggatgata ttgcgaaatt agcggaggat atcgtcggtc
     2161 gtgccatgaa gcaaggtgtg cggggggctt tcgtggcggg agaattcacg ttgacctatg
     2221 cgttgataca agcgctttgc aggcaaaata ttcgttgctt tacggcaacc acgaaacgcc
     2281 aggcctccga ggtcgttgcc cccgatggga gcgtcacccg ctcgtcggtc ttcgctttcg
     2341 tgaggtggcg ggaatacgtt tgacccaacg tccatgaaag ggcccattct gtcagaaata
     2401 gtggtatttt cacgtcattt gcaaaaaagt cggttgcgcc gggtgagcga attgcagtag
     2461 cctatcttcc gtgttgggcg cgactgactt tcaggggacg gcggtggtct gaaggatgga
     2521 gaaaggatgc gatgacgggc tttgaacttc acgcgtggcg aattcgcatt cgagcgctcg
     2581 aaaacctgga gctgccctgt cttcccacgg tggccttgcg cagcgtgacg ggcatggctc
     2641 ttcgggagat gagttgcgtt cgtagggagc gcacgtcctg cggtggatgt ccggagcaag
     2701 aagactgtgt gtattcacga cttgtcgagc ccgcgggcaa gggtcagtcc cctggtgtga
     2761 cggatgtggc accaccgccg ttggtgatgg ctccggaagc tcgaacgctc gacgatgccc
     2821 ccattcgtct gagcaaaggc gccacgatcg atgtgcgggc tgtgtttatg ggtggagcca
     2881 gggtgcatgc gtctttgtgg ctgacggcgc tgcgttctgg gcttgcgagg ggggtgggga
     2941 aagagcgtcg ggcgctggag gtggtggacg cgcagccgtt ggacgtcacg ttctgtgctg
     3001 cggggaaaaa ggcggaaatc gagctttgga ctcccttgcg gctcaaaagc caggggaaaa
     3061 tccggtcgag gttcgaggcg ggggatttcg tgcaggggct gatgcatcga gtcactctct
     3121 tatcggaaac gtatcgactg actcgacccg cggggttttc ggaggactct gttcgcctga
     3181 agcattgcga tggtcgtgtg attcatgtac ggcgctggtc agggcggcaa aaagccagca
     3241 tgaacttgcc tggattgacg gggacgctgg tgctcgaagg ggagttggag gggttgtggc
     3301 cgtggcttgc gctcggtgaa tgggtgcaga tgggcaaagg gacatcgatg gggtttggcc
     3361 ggtaccggct tcgggcgatt ggttaggaga aaaaagatga atcaacggga gcgagacgcg
     3421 cgattttggc aggccaaggt tgccgcgtgg gtgcatgatc cggcggagaa agcgctcgtg
     3481 ctgctgcggg atccccaggg gcatgagggt ggaacttcga agaagttgcg ggaagagctt
     3541 ttcccccagg gccttgatga aagcttgaaa aagctggtca aggaagcgga ccactgggcg
     3601 gcggcggcgg atcggccgca atttcctcgc tcggcgcacg atggtcgcta cgcttcctgg
     3661 agccaggtgg atttcgtgag cgagccacaa ctcattcatc ccctttccgg tgatagtgtc
     3721 tcgttgcagg atttcgagga catcgacaag gaacacatca aagccgtgtc tttcgaccac
     3781 ttgtctgacc ttatccacaa cgagaacggg gccatcgatt accgcagaac catgttggcg
     3841 ttgtggcgtt ttggttcgga aagccccgcg cggggaatcg gcgcgctgtg gtccatgctt
     3901 cccgccgata cccgcacccc ggaccactcg atctgggagc atttgcggct ttgttcggca
     3961 tacgcggggg ctacctgcga tgggcaagca tcgctgttat tgctaagcct cgggccggtc
     4021 caggggttca ttgcccaggc tcggtcgacg tcggatttat gggcggggtc tcatttgctt
     4081 tcgaggcttt cctgggaggc gatgcgtgtc gtggcggaat ggtgcggtcc ggacgccatt
     4141 ctatttccca atttgcatgg tgttcccatc gtggatcttt ggctggtgga gcaggggttg
     4201 gatttttcgc gatcgaaggg cattttcccc gattggatga ggttcgcttc cgatgccaac
     4261 cccctattca tcgcggcgct tcccaaccga ttcttggcct tggtgcccga atccattgcg
     4321 gaagatcttg cacgagaagt acgaacacga gttcgagaca aggctaagtc gttggcttgt
     4381 gaggcatggc gttgcgtagc gacactggcg gaactcgacg agggggatgg tctttcccaa
     4441 attgccgatc aactccatgg gtttcccgaa gttcattggg cggtgattcc cgcttccctg
     4501 gcgaaaagct cctcggactt acaaaaagcc atggagccgt tttttcccgc cgacgatact
     4561 ccccccggat tcctcggttc ttccctgttt cgggctctgt cgaaagatac aacgctggag
     4621 ggcacggtct ttttcccccc tgggcccggc actctttacc cggctttgta cgacctcacg
     4681 gagcgcaccc acgccgcggc caaaacgtcc cagacctttt ccgcgctcaa ccaggaaggc
     4741 taccgctgca gcctttgtgg agaaagggaa tggctgacca ccgatcgcaa gctgctggcc
     4801 taccacaccc aggacaacca accaggttcg ccgtggccga tcgtcgccca aaatccccgg
     4861 gcttgggcca agccaggaga gcgtttatgc gccttgtgct gcacgaagcg tctctggccg
     4921 acgctctttt ccgaagaact caaaacgatc ctcgaaaaaa cacccgaaac cgtggatatt
     4981 ccgctgaagt caacggtgat aagcggtcag atccaacgct acgtggtctc gacacatacg
     5041 atggctcttg cctcgacgtt tcgtgagatg gctcggggtt tcattaaaaa taataataaa
     5101 ctcaaagagt tagcaggcca tctcgaggaa tacaggcaca ctgccttgcc gcgtcagttg
     5161 gctcatgcct ccgtctccga cgatctggtc
//
LOCUS       DAOSOR010000433         5180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_16159_length_5180_cov_3.537561, whole genome shotgun sequence.
ACCESSION   DAOSOR010000433 DAOSOR010000000
VERSION     DAOSOR010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16159_length_5180_cov_3.537561"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /gene="otsB"
                     /locus_tag="PK710_18975"
     CDS             <1..791
                     /gene="otsB"
                     /locus_tag="PK710_18975"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HOT11869.1"
                     /translation="GLPTFWTMLDASPHRLLALDYDGTLAPFRVDRRQAFPLPGVVQA
                     LEALASRTDTKLAIISGRPLRDLKKLLRLSEGAGLTWVGSHGFEGQRADGETWTRRLT
                     AMQSENLLSARRAVEALGHGARLENKPSALAFHTRGMESEEAKSLEKTVYSVWTALAD
                     TAGLRIHAFDGGVEIRVTTADKGQALKEWVNQQAAGAFVVYVGDDRTDEDAFEVAQGF
                     GGIGIHVGYGETCALGTLADCEAVLAWLQRWARMPTYGKVGGIT"
     gene            788..2233
                     /locus_tag="PK710_18980"
     CDS             788..2233
                     /locus_tag="PK710_18980"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HOT11870.1"
                     /translation="MNGSRDFRRLVVVSNRLPIVLGRDEKGQWAARPGEGGLVTALAP
                     VLRNRGGVWIGWPGTDDREALDKVKATSAQAGYQLLPVPLTAEDEKGYYHGFSNETIW
                     PLFHDLVGHCRFEPAHWRKYQEVNQRFARTVLETTTQEDYVWVQDYHLMCLGSMLREK
                     GVKRRLGFFLHIPFPAVDIFSKLPWRKQILEALLAYDLIGFQTARDRRNFIQCVRSLL
                     QGVQHVGRGYLGAIRVGERQVLVGHFPIGIDAKRFASEAASAPVAEQAWYIHELLPKR
                     QIVLGIDRLDYTKGIPERLRAVGNALERFPELHRQLTFVQVVVPSRVNVGDYRQQRRT
                     IEQLVGKINGKFTTAGWTPVHYIYRALSWHELLAYYRTSEIALLTPLKNGMNLIAKEY
                     CVCSVESGVLILSEFAGAARQLQGALLVNPNDIEATADALRQAFHMPAAERKRRIRKL
                     QREVDRHDVFWWVDLFLNAAFGRRLADFPLA"
     gene            2325..3131
                     /locus_tag="PK710_18985"
     CDS             2325..3131
                     /locus_tag="PK710_18985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11871.1"
                     /translation="MKYFRWISVGSWLLLGCTTEPDGSPASAGASGNGADAEPGGGHG
                     GENGTPGGHGGEAGQGAVGGFGAAAGQSGAAGSAGIAGSAGMAGQTGSGSTGLYEPCT
                     TTAECLPGLHCQSHVPEPQGGLCSHACTSHDDCENTASGKGICHPVTSTCVLGCGLNP
                     GQCPDGLVCTDSQFCVEKSSLAASKNTGQSCATELECLHGAACISGEHTSAYCSPPCQ
                     NDEDCKTGAPEAVGSCKQASGLTFCIYLCGIMANGAACPGDLTCEFGMCR"
     gene            3150..3503
                     /locus_tag="PK710_18990"
     CDS             3150..3503
                     /locus_tag="PK710_18990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11872.1"
                     /translation="MRRTIAFLLLVIGTGCGSDDDSQESAESDFIARCNAAYAISCDK
                     GFACDSVHYKGRFNSADHCKHEMSKSVQESSKKFSTSALKDCADTCDLMKNDIQSATC
                     EELDRMVFNDYSCGP"
     gene            complement(3569..4219)
                     /locus_tag="PK710_18995"
     CDS             complement(3569..4219)
                     /locus_tag="PK710_18995"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HOT11873.1"
                     /translation="MADPKKSGGGIVGKKTRRTPRTLGVQAYIPETAKGMKVLMKHFF
                     QNTKELMLGQRNDPVLEALDDGINTISYPEQKRPYPLRNRGLHRLTVREDGSPRCVAC
                     LCCSTACPAQCIFIEPAEYPEGHERRGYERYPARFVIDELRCIFCGFCVEACPCDAIR
                     MDTGEHAVPYDSRDQFLFDKDLLLSFTGRDGTRKNINPRHEPGDPTHPGITRLKKH"
     gene            complement(4271..>5180)
                     /locus_tag="PK710_19000"
     CDS             complement(4271..>5180)
                     /locus_tag="PK710_19000"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOT11874.1"
                     /translation="ILFIFAVSGIGILGAAIGGWASDNKYSLLGALRAAGQLVSYEIT
                     LGMTLIGLLMIYSTFRLDDMVRWQGNNAWGVFVQPLAFFLFFIAATAETKRIPFDLPE
                     GESEILGYFTEYSSMKFGMYFFSEYIEVVTSSAVMITLFFGGWNLPFLHRDGLTIAIG
                     DAVLLKHAMTHGSVVALQVVAFFLKVAAFAWLQVVVRWTLPRFRYDQLMKMCWRMLLP
                     LSIANVFVTAILLLIIDNASLMAKNALGVAADLTQLTTMIAFVVAIVAMARWLLAPAK
                     KHRVVLTSTAKMVAEQGGTRHSPMQA"
BASE COUNT         1130 a         1339 c         1587 g         1124 t
ORIGIN      
        1 caggattacc tacgttttgg accatgctag atgccagtcc gcatcgtctt ctggctctgg
       61 actacgatgg caccttggcg ccttttcggg tggaccgtag gcaagcgttt cccttgcctg
      121 gcgtggtgca agcgctcgag gcccttgcct cgcgaaccga cacgaaactg gcgatcatct
      181 cgggccgtcc gttgcgggat ttgaagaagc tgttgcggct ttcggagggt gcgggcctca
      241 cctgggtggg aagccatggc ttcgaggggc agcgagccga tggggaaacc tggacgcggc
      301 gtcttacggc gatgcaatcg gaaaacctgc tgagcgcacg acgggccgtg gaagctcttg
      361 gtcatggcgc caggctggag aacaagcctt cggcgcttgc ttttcacacg cgaggcatgg
      421 agtccgaaga ggcaaaaagc ctagaaaaaa cagtatattc cgtctggacg gctcttgcgg
      481 ataccgccgg acttcgtatt catgctttcg atggaggcgt ggaaatccgc gtcacgaccg
      541 ccgacaaagg acaagccctc aaggagtggg tgaatcagca ggcggcagga gctttcgtgg
      601 tctatgtcgg cgatgacagg actgacgaag acgctttcga ggtcgctcag gggtttgggg
      661 gtattgggat tcacgtgggt tatggggaga cctgcgcctt gggcacacta gctgattgtg
      721 aagcggtatt ggcctggcta caacgttggg cgaggatgcc aacctatgga aaggttgggg
      781 gaattacatg aatggctcgc gggattttcg acgattggtg gtggtatcga atcgacttcc
      841 gatcgtattg gggcgagatg agaagggaca atgggcggcg cgtccgggag aaggaggatt
      901 ggtgacggcg ttggcgccgg ttcttcgcaa tcgtggaggc gtgtggattg gttggccggg
      961 cacggatgat agggaggcgc tggacaaggt gaaggcaaca tccgcgcaag cgggttatca
     1021 attgcttccc gtgccgctga cggcggagga cgaaaaaggg tattatcatg gtttttccaa
     1081 tgaaacgatt tggccattat ttcatgacct ggtgggtcat tgtcggttcg aaccggctca
     1141 ttggcgaaag tatcaggagg tcaaccagcg atttgcccga acggtgttgg agactaccac
     1201 ccaagaggat tacgtctggg tccaggacta tcatttgatg tgtttgggaa gtatgcttcg
     1261 agaaaagggg gtgaagcgtc gcctcgggtt tttcttgcat atcccttttc ccgcggtgga
     1321 tattttttcc aaattaccat ggcgcaagca gatcctcgaa gccctgttgg cctacgatct
     1381 cattgggttt caaaccgccc gagaccgtcg caatttcatc caatgcgtgc gcagcctcct
     1441 tcagggagtc caacacgttg ggcgaggata cctcggagcg attcgggtgg gagaaagaca
     1501 ggttctcgtg ggtcacttcc ccatcggaat cgatgccaaa cgctttgcgt cggaagcggc
     1561 ttcggctccg gtggctgagc aggcctggta tattcatgaa ttgcttccca aaagacagat
     1621 tgtgctcggc atcgatcgac tcgactatac aaaaggaatt cccgaacgat tacgtgcggt
     1681 tggcaacgcg ctggagcgtt ttccggagtt gcatcggcag cttacgttcg tgcaggtggt
     1741 ggtgccgagc cgggtcaacg tgggggatta tcgtcaacaa cgcagaacga tcgagcaact
     1801 cgtaggtaaa atcaatggaa aattcacgac cgcgggctgg actcccgtcc actatatcta
     1861 tcgtgccctt tcctggcatg aattactcgc ctattaccgc acgagtgaga tcgcacttct
     1921 gacacctctc aaaaacggca tgaacctcat tgccaaagag tattgtgttt gtagtgtcga
     1981 gtcaggcgtg ctcattctca gtgaatttgc cggcgctgcc cggcaactgc agggagcctt
     2041 gttggtcaat cccaacgaca tcgaagccac cgcggatgcc cttcggcaag cgtttcacat
     2101 gcctgccgcc gaacgaaagc gtcgcattcg aaagcttcag cgggaagtgg atcggcatga
     2161 cgtgttttgg tgggtcgatt tattcctgaa cgccgcgttt ggacgtcgcc ttgccgattt
     2221 tccgcttgca tgacgattcg caccctcttc acgagtacca aaacaaaata agtcggagcc
     2281 aattgcccga tacgttctgc ccgacgtatc ctgagggtat gtgggtgaag tatttccggt
     2341 ggattagcgt tgggtcatgg ttgctgctcg gatgcacgac cgaacccgac gggagtcccg
     2401 caagcgcggg ggcgtcgggg aacggggcag atgccgagcc tggcggtggg catgggggtg
     2461 aaaacggcac cccaggcggc catggtggcg aagccggaca aggtgccgtg ggtggtttcg
     2521 gagccgcggc aggtcagtcc ggcgcggcgg ggtcggcggg aatcgcagga tcggcaggga
     2581 tggcgggaca aaccggctca ggttcgaccg gcctttatga accatgcacc accacggccg
     2641 aatgcttgcc ggggctgcat tgtcaaagcc atgttcccga accgcaaggg ggcctgtgca
     2701 gccatgcttg cacaagccac gatgattgcg aaaacaccgc gagtggcaaa ggaatctgtc
     2761 atcccgtcac ctccacctgc gtcctcggtt gtgggctcaa tcccggacag tgccccgacg
     2821 gcttggtgtg tacggactcc caattttgcg tggagaaatc ttctttggcg gcttcgaaaa
     2881 acaccggcca atcctgtgcg acggaactgg aatgtcttca tggggcggct tgtatctccg
     2941 gcgagcatac gagcgcgtat tgttctcccc cgtgtcaaaa cgatgaggat tgcaagacgg
     3001 gggcaccaga agcggtgggc agttgtaagc aagccagtgg attgactttt tgcatttatc
     3061 tatgcgggat catggcgaac ggcgcagcct gtcccggcga tctcacctgc gaattcggga
     3121 tgtgtcgata atcccaacta cggaggccca tgcgacgcac aatagcattt ctgctcctag
     3181 tgattggtac gggatgcggt tccgatgatg attctcagga aagcgcggaa tcggatttca
     3241 tcgcgcgctg caacgcagct tacgcgatat cctgtgacaa agggtttgcc tgcgactccg
     3301 tccattacaa gggtcgattc aacagcgctg accattgcaa acatgagatg tccaaatcgg
     3361 tacaggaatc atctaaaaaa ttctcgacct cggcgctcaa ggactgcgcc gacacctgcg
     3421 acctcatgaa aaacgacata cagtcagcga cttgcgagga attggatcga atggtgttca
     3481 atgactactc atgcggtccg taatccctat tgggatatcg acgaggagcg gtgaggcaga
     3541 tggggtccgg tggccccggc gagagccttc agtgtttttt gagtcgggtg attcccggat
     3601 gtgtgggatc gcctggctca tgacgcggat tgatattctt acgcgtgcca tctcgccccg
     3661 tgaacgatag caacaggtcc ttgtcgaaaa ggaattggtc gcgagagtca tagggcacgg
     3721 cgtgttcacc tgtgtccatg cgaatcgcat cacagggaca tgcctcgaca cagaagccgc
     3781 agaagatgca tcggagttca tcgatcacga atcgggcggg ataacgctcg tagccgcgtc
     3841 gttcatgccc ttccggatac tccgccggtt cgatgaaaat gcattgcgcc gggcaggcgg
     3901 tagaacagca gaggcaggcc acgcagcggg gcgacccatc ctcccggacc gtgagacgat
     3961 gcaggcctcg gttacgcaat gggtagggtc gcttttgttc cggataggag atggtattga
     4021 tgccatcatc caacgcttcg agcacggggt cgttgcgttg cccgagcatg agttccttgg
     4081 tgttctggaa aaagtgcttc atcagcactt tcattccttt ggctgtttcg gggatatacg
     4141 cttgaacgcc aagggtccgt ggtgttcgtc gggttttctt cccgacgatt cctccacctg
     4201 attttttggg gtcagccatg gtgattgggt gtccctttcg tgcgcatggt cagccatgcg
     4261 tttgcttcga tcacgcttgc atgggggagt gcctggtgcc tccctgttcc gctaccattt
     4321 tcgcggtgga ggtgagcacg acgcgatgct ttttcgccgg tgcgagcaac catcgagcca
     4381 tggcaacgat ggccaccaca aaagcaatca tcgtggtgag ttgggtgaga tccgcggcca
     4441 cgccgagagc gtttttcgcc atgagactcg cgttatcgat gatgagcaag aggatcgcgg
     4501 tcacaaacac gttcgcaatg gataggggca aaagcatccg ccagcacatt ttcatgagtt
     4561 ggtcatagcg gaagcgcggc aaggtccagc gcaccaccac ttgtagccag gcgaaagccg
     4621 ccactttgag gaagaaagcg acgacctgca aggccaccac cgagccatgg gtcatggcgt
     4681 gcttgagcaa caccgcatcg ccaatggcga tggtcaagcc atcccgatgc aaaaagggaa
     4741 gattccagcc gccaaaaaac agagtgatca tcacggccga ggaggtgacc acctcgatgt
     4801 actccgagaa aaagtacatg ccgaacttca tgctcgagta ttcggtgaag tagccaagga
     4861 tttcactctc tccttccggc aaatcgaaag gtatccgttt ggtctccgcg gtcgcggcaa
     4921 tgaagaacag gaaaaaagct agcggttgga caaacacgcc ccacgcgttg tttccttgcc
     4981 accgcaccat gtcatcgagc cggaacgtcg aatagatcat gagcaacccg atcaacgtca
     5041 tgccaagggt gatttcatag gacacgagct gaccggcggc gcgaagggca ccgagaaggg
     5101 aatacttgtt atcgcttgcc catccaccga tggcagcgcc caaaatcccg atgccggaca
     5161 ccgcaaagat gaagaggatg
//
LOCUS       DAOSOR010000434         5140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_16325_length_5140_cov_4.384071, whole genome shotgun sequence.
ACCESSION   DAOSOR010000434 DAOSOR010000000
VERSION     DAOSOR010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16325_length_5140_cov_4.384071"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PK710_19005"
     CDS             <1..460
                     /locus_tag="PK710_19005"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOT11875.1"
                     /translation="TCFVYPLASDPSARVMGWAGVAALILFFVPFELTYEILYDFRDI
                     KGDQAQGVPTYPVVHGQERARQIIDGLLVVSALVLGVALAAGVLGVREGLMMAAPLIQ
                     FFFYRPRVRRGVTTPDCVWLTHLGTAQLVMFVVGTAVWGKLGLPANIFLH"
     gene            550..3231
                     /locus_tag="PK710_19010"
     CDS             550..3231
                     /locus_tag="PK710_19010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOT11876.1"
                     /translation="MPSFATHPYASFIQEIEKPARYVGGEWGQVRKPWDRTRVRICLA
                     FPDIFDIGMSHLGTRILYAELNEAPDMLCERAFCPWTDMERALRERGLPLLSLENAKP
                     LSAFHVVGISLQHELVYTNVLTLLDLGGIALRSSERADSDPLVLGGGSVASHPEPVAD
                     FFDAFVIGDGEKKAAEVARRWVEDLERGVGREERLRHLASLGGVYVPSLYEHRFDARC
                     GREVVDRPRYSEAPLPVVRATVPELGMFPTRFPTGGPEAVFDRLSIEIARGCSQGCRF
                     CQGGMIFRPERERDPATIVECVLQSLAHSGQDEISLTSLSPADYTAMAPLVAQLSHHP
                     SLSHVALNVSSLRAYGLDDPTLDEIRRVRVSNLTFAPEAGTQRLREVINKNVTEEQLL
                     ETVRRVVRRGWERIKLYFMIGLPTETQEDVEAIVQLTSRVRAEARAARESKGRSVQVT
                     ASASTFVPKPHTPFQWASMVDLDTVKEKHRRIVVWAREHKVDIKMHEPHGSVLEGLLS
                     RGDRRLGPVIEDAWRAGSRFDGWEGNVKWGIWLAAMERHDLQLIDYIKEIPNEATTPW
                     SHIHVGVSEEFLRQEWEKAQRFKHTLPCLRPADPASKSDKLVCYHCGLDCDLEQAARI
                     RSQRMSAPVADRAPAAMVASCDTVQYRFCYEKIGRASLLGHLDMVREMPRIMRRAGFA
                     VVYTQGFHPKAMMGFGPALRLGVPSFEEYVDVKMQELANPEEGLAKLNAICPEGIRFV
                     RMVRLPDKAPSLVQAIGEARMLLGIDPSAVEARGGVSWLKERLGELMSSESLPWIRNH
                     KGKERTLDLRPWILELRMGTEDDAVRMSEAGIPGSWVMVRLHHALTAQGSVRIDEVVA
                     LMGEREALPYRAVRVALTKTGGRSVMPSAEALGVEVL"
     gene            complement(3253..4170)
                     /gene="hemH"
                     /locus_tag="PK710_19015"
     CDS             complement(3253..4170)
                     /gene="hemH"
                     /locus_tag="PK710_19015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HOT11877.1"
                     /translation="MPDLGILLLSHGSPAHLDDIPEFLANIRRATPPQPIVQAVRARY
                     EAIGGSSPLPEITRAQAHAIEKKTGLPCLIATRMWHPSFEQVLREVSSAQSPTSLIVL
                     SAAPFSAHVYTEALQATALRLQSQGLPIPTLHPAPCWGNHPRYIQGWADATVTVLDQL
                     GIRKTARAVLVPTAHSLPLRIVQAGDPYPHLIHTAVEDFLNRLATNALPSILAFQSQG
                     MTEEPWLGPDLPTVFERAKATGAEGVILVPIGFPTEHVETLYDLDIEAKQIARQQGLW
                     LERVPCLNTAEPLIDALCDLVDQTRNRLS"
     gene            complement(4174..>5140)
                     /gene="hemE"
                     /locus_tag="PK710_19020"
     CDS             complement(4174..>5140)
                     /gene="hemE"
                     /locus_tag="PK710_19020"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HOT11878.1"
                     /translation="PVWFMRQAGRYMAEYRELRAKYSMLELCKTPELAVQVTLQPLRL
                     GVDAAIVFSDILLPLEPMGVPVHFSKNDGPVLEPIRCQNDVDRLRIIDPQESLDYVLE
                     SIRILRRELNVPLIGFAGGPFTLASYLVEGGRTNTFVRTRRLMLAEPHIWHALMEKLS
                     EVVRRHLRAQIDAGAQAVQLFDSWIGQLSPDDYTDSVASHVHKILHNIEMLDVPVIHF
                     GTGTSTLLEAMAKTGGTVLGLDWRIPLDQGWRRIGWNRSVQGNLDPTTLFAPRHIIQQ
                     RASAILQAAANRNGHIFNLGHGILPETPETEVQALVDFVHQSSQR"
BASE COUNT          983 a         1183 c         1840 g         1134 t
ORIGIN      
        1 gacgtgtttc gtatatccct tggcatcgga tccctcggcg cgggtgatgg ggtgggctgg
       61 ggtggccgca ctgatccttt tttttgtccc attcgagcta acctacgaaa ttttatacga
      121 ttttcgtgat atcaagggcg atcaggcgca aggggttccc acctatccgg tggttcatgg
      181 gcaagagcga gcgcgtcaga tcatcgatgg tttgctcgtg gtttcggcgc ttgtcttggg
      241 ggtggcgttg gcggctggcg tgcttggcgt gcgcgaggga ttgatgatgg ctgccccgct
      301 gattcagttt tttttctatc gtccacgggt tcgacgagga gtgactacgc cggactgtgt
      361 ttggctcacg catttgggca cggcccagct cgtgatgttt gtggttggca cggcggtgtg
      421 ggggaagctg gggttgccag ccaacatctt cttgcactag ggaaagcgtc tgtcccgcgc
      481 gagcatcgag cgaaaacggg tggtccgggg tctcgaagcg tgcgggaaac atggtcgagc
      541 gtcaacgtca tgccttcgtt cgcaactcat ccttacgcct cattcatcca ggaaatcgag
      601 aagccggccc gttatgtggg aggggaatgg gggcaagtgc gcaagccgtg ggatcggact
      661 cgcgtgcgaa tatgccttgc atttcccgat attttcgaca ttgggatgag tcatctgggc
      721 acgcggatct tgtatgcgga gctgaatgag gcgccggaca tgctttgcga gcgtgcgttt
      781 tgtccatgga cggacatgga gcgggcattg cgtgagcgcg gtctgccctt gctatcgctg
      841 gaaaatgcga agcctttatc ggcttttcac gtggtgggca tctcgttgca gcacgagctt
      901 gtctacacca atgtccttac gcttctcgat ctgggaggga ttgcgcttcg atcatcggag
      961 cgagcggaca gcgatccgct ggtgctcgga ggaggttcgg tggcgtcgca tcccgaaccg
     1021 gtagctgatt ttttcgatgc cttcgtgatt ggagatgggg aaaaaaaagc ggcggaggtg
     1081 gcgcgacgat gggtggagga tctcgaaagg ggggtagggc gtgaagagcg tcttcgccat
     1141 cttgcgagcc ttgggggtgt ttatgttcct tcgctctacg agcatcgctt cgatgcgcgt
     1201 tgtgggcgcg aggtggtcga tcgaccgcgg tattccgaag cgcctttgcc tgtcgttcgc
     1261 gccacggttc ccgagcttgg gatgtttccc acgaggtttc ctaccggcgg gccggaagcg
     1321 gttttcgatc gtctatcgat cgagatcgct cgcggatgca gccagggctg tcgattctgc
     1381 caggggggaa tgatctttcg gccggaaagg gagcgcgatc ccgcgaccat cgtggaatgc
     1441 gtgctgcaat cgctggcgca tagcggccaa gacgagatct cactcacatc gctttcgccc
     1501 gcggactaca cggcgatggc ccctctggtg gcccaactct cccaccatcc gtccctttcc
     1561 catgtggcgc tcaatgtatc gtcgttacga gcttatggtc tcgatgaccc gacattggac
     1621 gagatacgtc gggttcgtgt gagcaacttg acgttcgcgc cggaggccgg gacccagcgc
     1681 ttgcgagagg tgatcaacaa gaacgtgacg gaagagcaat tgctcgagac ggtgcggcgg
     1741 gtcgtgcgac gaggttggga acggatcaag ttgtacttca tgattggtct tcccaccgag
     1801 acacaagagg atgtggaagc gatcgtgcag ttgacgtcgc gtgttcgagc ggaggcgagg
     1861 gccgcacgcg aaagcaaggg aagatcggtt caggtgacgg catcggcttc cacgttcgtt
     1921 cccaagccac acacaccgtt tcaatgggcg tcgatggtgg atctggatac ggtgaaggag
     1981 aagcatcgac gcatcgtggt gtgggcgcgc gagcataagg tggatatcaa gatgcatgag
     2041 ccgcatggca gcgtgctgga ggggttgttg tcgcgagggg acaggcgttt gggccctgtg
     2101 atcgaagacg cctggcgcgc gggctcccga ttcgatgggt gggaaggcaa tgtgaaatgg
     2161 gggatctggc tggcggcgat ggagcgccac gacctgcaac tcattgatta tataaaagaa
     2221 attccaaacg aagccacaac gccgtggagt catatccatg tgggggtaag cgaggagttt
     2281 ttgcgtcagg agtgggaaaa ggcccagcga ttcaagcaca cgttgccgtg tttgcgtccc
     2341 gcggatcccg cgtcgaagtc ggacaagctc gtgtgttatc attgcgggtt ggattgcgat
     2401 ctggagcaag cggcgcggat tcgttcgcag cgcatgtctg cccccgttgc cgatcgagcc
     2461 ccggcggcga tggtcgcgtc ttgcgatacg gtgcaatacc gattttgtta tgaaaagatc
     2521 gggcgagcgt cgttgttggg tcatctcgac atggtccgag agatgcccag gatcatgcga
     2581 agggctggtt ttgcagtcgt atacacgcaa gggtttcatc ccaaggcgat gatgggtttc
     2641 ggtccggctc ttcgtcttgg tgttccgagt ttcgaagaat atgtggatgt gaagatgcag
     2701 gagcttgcga atcccgaaga aggattggcg aagttgaatg cgatctgtcc ggaggggatt
     2761 cgcttcgttc ggatggttcg attaccggac aaggcgccat cgttggttca agccattggg
     2821 gaagctcgga tgctattggg gatagatcca agcgcggtcg aggcgcgcgg tggagtgtcg
     2881 tggttgaaag agcgcctggg agaattgatg tcttcggaat cattgccgtg gattcgaaac
     2941 cacaaaggaa aggagcggac cctcgatttg cgtccatgga tcttggagct tcgaatggga
     3001 acggaagacg atgcggtgag gatgagcgaa gcggggatac cggggtcctg ggtcatggtc
     3061 cgtttgcatc atgcgctcac ggcacagggc agtgtgcgaa tcgacgaggt cgtggcgttg
     3121 atgggtgagc gagaggcgct gccctatcgc gcggtgcgag tggcgttgac gaaaacgggc
     3181 gggagaagtg tgatgccgtc cgcggaagct ctcggggtcg aggttttgta gcgatggtcg
     3241 aagcagggat cgtcaggaca agcggttgcg cgtttggtcg acgagatcgc agagggcatc
     3301 gataaggggc tcggcggtgt tgagacaggg cactcgttcg agccagaggc cctgttgtcg
     3361 tgcgatttgt ttggcttcga tatcgaggtc atacagggtt tcgacgtgtt cggtgggaaa
     3421 gccgatgggc acgaggatga cgccttcggc accggtggct ttggcgcgtt cgaaaacggt
     3481 gggcaagtcg gggccgagcc atggttcctc ggtcattccc tggctttgga aagcgagaat
     3541 ggatggcagg gcgttggtgg cgaggcgatt caagaagtcc tcgacggcgg tatggatgag
     3601 gtgaggatag gggtcgccgg cctggacgat gcgcaggggc aagctatggg cggtggggac
     3661 gaggacggct cgcgcggttt ttcgaatccc gagttggtcg aggacggtga cggtggcatc
     3721 ggcccaaccc tggatgtagc ggggatggtt gccccagcag ggagcggggt gaagcgttgg
     3781 gatgggcaag ccctgggatt gcaggcgaag ggcggtggct tggagggctt cggtatatac
     3841 gtgcgcgctg aaaggggcgg cggatagaac gatcaaggac gtgggtgatt gtgcggaaga
     3901 gacttcacgc aggacttgtt cgaaagatgg gtgccacatg cgcgtggcga tgaggcaggg
     3961 gaggccagtt tttttctcga tggcgtgggc ctgagcccgg gtgatttcgg gcaggggaga
     4021 ggagccaccg atggcttcgt agcgggcgcg gacagcctgc acgatgggct gtggtggcgt
     4081 tgcgcgacga atattggcta aaaactcggg gatatcgtcg aggtgagcgg gcgagccatg
     4141 ggagagcagg aggataccga ggtctggcat ggtttagcgc tgggaggatt ggtgaacgaa
     4201 gtcgacgagg gcctggactt cggtttcggg tgtttctggg aggatgccgt gaccgaggtt
     4261 gaagatatga ccattgcggt ttgcggcggc ctgaaggatg gcggaagcgc gttgttggat
     4321 gatgtgtcgt ggggcgaaca aggtagtggg gtcgaggttg ccttggacgc ttcggttcca
     4381 gccaatacgt cgccagcctt gatcgagggg gatacgccag tcgaggccga ggacggttcc
     4441 accggttttg gccatggctt cgaggagggt tgaagtacct gtaccgaaat gtattactgg
     4501 cacatctaac atctcgatat tatgtaatat tttatgaaca tgagaagcta cactatcggt
     4561 atagtcatcg ggagagagct ggccgatcca ggaatcgaag agttggacgg cctgagcgcc
     4621 ggcgtcgatc tgggcgcgaa gatgacgtcg aaccacttcg gataattttt ccatcagcgc
     4681 gtgccagata tgcggttcgg ccagcatgag gcgtcgggtg cgcacgaagg tattggttct
     4741 tccgccttcc accaggtagc tggcgagggt gaagggccct cctgcaaaac cgatgagcgg
     4801 gacgttcagt tcccggcgca ggatacgaat ggattcgaga acgtaatcca ggctttcttg
     4861 ggggtcgatg atgcgaaggc ggtccacgtc gttttggcag cgaatgggtt cgagcacggg
     4921 tccatcgttt ttggagaagt gaacgggtac gcccatgggt tcgaggggca ggaggatatc
     4981 ggagaagacg atggcggcat cgacgcccag gcgcaggggt tggagggtga cctgcacggc
     5041 aagttccggg gttttgcaca gctccagcat ggagtatttc gcgcgcaatt cgcgatattc
     5101 ggccatatat cgtccggctt ggcgcatgaa ccacaccggg
//
LOCUS       DAOSOR010000435         5137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_16347_length_5137_cov_4.152499, whole genome shotgun sequence.
ACCESSION   DAOSOR010000435 DAOSOR010000000
VERSION     DAOSOR010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16347_length_5137_cov_4.152499"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..793)
                     /locus_tag="PK710_19025"
     CDS             complement(41..793)
                     /locus_tag="PK710_19025"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOT11879.1"
                     /translation="MTQHDRTERLLGKEALSRLRAACVTVFGLGGVGSSCVEALARSG
                     LGHLRLVDHDTIHESNCNRQLHALHSTVGLPKAQVMKQRVLDIDPTLQVEAFCTFFAQ
                     DTANDLLEPPTHCVVDAIDAMGPKVELLSRCKSLGIPVISALGAGGRLDPTQIRVAPL
                     DQTVGDPLAARVRKLLRRRGTLDGITAVYSLEPPRIAATAPILSRDLFRGRQRIIQPS
                     MLMVPAAMGLAIAYEVIRTIALDPSLNDGPTS"
     gene            complement(790..1539)
                     /locus_tag="PK710_19030"
     CDS             complement(790..1539)
                     /locus_tag="PK710_19030"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOT11880.1"
                     /translation="MFDSHCHLTDLEEPDQALKDARHGGVMSMLTCGYHEPSNQLTCA
                     LHERHPSLPFALGLHPWFATQDLENVLQLIETHHPTAVGEAGLDLWGDPPCHPVERQI
                     QVLEAQLQIAVRLGLPVTLHSRKAVDLLYSVLRNHPGVQGALHAFSGSYEQARRFLDL
                     GMYIGVGGSVTRSRAQRVRHCARSIPLDRILLETDAPAIGMDGVEPPHVRPAHLVIVR
                     DALATLRQQPAETIEAATDENASRLFGISFS"
     gene            complement(1657..4137)
                     /locus_tag="PK710_19035"
     CDS             complement(1657..4137)
                     /locus_tag="PK710_19035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11881.1"
                     /translation="MLRRASFVFISALCWSSLASCSSGSPVTQSESGDITPPQGSQSG
                     VGQPCDEQNRCRTGLECVHGQCAPGKSQPENGPCVISAECQEGLYCTAHGRCATAGSG
                     QLGEACTSDGDCRDGLKCGFKNFALVCIEQGDHDVGQPCVSSADCFGGLACVHDVCAK
                     TPPGAPSFGMPSWAGETCSDDLSQPAVAYFEVPRLSGNGDFYRIPFPNDIRTRDGHPD
                     LTGHPTPGASLLGFDPLEPYLRAIEKENVGFGIYPTVVFRFSNSVDWNSIDNGVNPGA
                     VQWVDITPTSPHYGYTLGWFSLANGMRTKYLCPNWLALRPPLGEPLQPGITYAVILSS
                     SIKRPDGNFVKRSPDFSAMLEDSVPVEQALIQPHSHYKVFRDYLADKGLDKSTLLNAA
                     VFTVGPVRDAAETMARSVDAAPAPTASSWVRCGNAPSPCPQASEDRACGQPNAKFDEL
                     HALVSLPIFQHGAAPYLEQGGQFEITGSTFTIARQEKVCLSLTIPKGIQAPEKGWPLV
                     VFSHGTGGSFRSHVSLGIAETMANEGIAVIGIDQVQHGPRRGESTESPENLFFNFANP
                     FAAKYNAMQGAADQLSLLRFVSMLSIDAPSSPTGSALHFDPNRFSFWGHSQGATQGAL
                     GIPYSTGIKGVVLSGHGASLLESLLSKTSPVNIAAALPFVLGDVDESFQLPGGSLHPA
                     LSLLQAWIDPADPLAHAKQMSITPPLPQTARHVFQPFGQRDTFSPGKTQLTYLIAARF
                     GRVADDPSTVVANPNDDNELAGVGLVTGNAGNASVEGAAVTILTRRYGVDDYDGHFVA
                     FRNQAARQDVTRFLVQSVSGQDPPTVGP"
     gene            complement(4250..>5137)
                     /locus_tag="PK710_19040"
     CDS             complement(4250..>5137)
                     /locus_tag="PK710_19040"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT11882.1"
                     /translation="QALRGRMRAFANIDWRGVNQPDFIDQQVALLEQAKRDGIVGIKI
                     FKALGLAVRDSQGRLIAADAPLLAPIFDACGKLGLIVAWHIGDPVAFFDPVTPDNERY
                     DELKLAPDWSFYGGDYPRHAELIAAQDRVLRRHPHTVFLLIHFGNYPENIDYVDRLLD
                     ACPNVFVDTSARVPEIGRHPAAKVRALFIKHQDRILFGSDFIAGPTGGMQLGSVSDRE
                     PTVADAREFYERHWRYFETSMPQIDHPTPSQGKWKVDAIGLPLDVLRKLYVTNARKLL
                     FSGEFPSPYRPSPSSDPPK"
BASE COUNT         1124 a         1327 c         1657 g         1029 t
ORIGIN      
        1 ggtgtttttc tgcaaacgga gcgcaggtgg ggaccggata tcaggacgtc ggtccatcgt
       61 tgagcgatgg atccagagcg atggttcgaa tgacttcgta ggcgatggcg agtcccatgg
      121 cagcgggcac catgagcatg gacggctgga tgattcgctg tcttccacgg aataggtcgc
      181 gggacagaat gggggcagtc gcggcaattc gcggcggttc caacgaatac acggcggtga
      241 tgccgtcaag ggtgccgcgt ctacgaagca gcttgcgaac gcgagccgcc aaaggatcgc
      301 caaccgtctg atcgagcggc gcgacccgta tttgcgtcgg atccagccgt ccacctgccc
      361 caagtgcgga aatcacggga attccaaggg atttgcaacg agacagcaat tcgactttcg
      421 gccccatggc gtcaatcgca tccacgacgc aatgggtagg gggctccagc aggtcattgg
      481 cggtatcttg cgcgaaaaac gtgcagaatg cttctacttg cagggttggg tcgatatcga
      541 gcacgcgttg tttcatgacc tgcgctttgg gtaaccccac ggtcgaatgc agtgcgtgaa
      601 gctggcggtt gcagttggat tcgtggatgg tgtcatgatc gacgaggcgc aggtgtccca
      661 atccgcttcg ggcaagggct tcgacacagc tcgaaccaac accgcccaag ccgaaaacgg
      721 ttacacaagc cgcgcgaagt ctgctcaatg cttccttacc caaaagccgt tccgtgcgat
      781 cgtgttgcgt catgaaaagc tgattccaaa caaacgagac gcgttctcgt ccgtcgcggc
      841 ttcgatggtt tcggcgggtt gctgtcgcag ggtagcgaga gcgtcgcgaa cgatcaccag
      901 gtgagcggga cgaacatggg gaggctccac accgtccatg ccgatagcag gagcgtcggt
      961 ttccaacagg attcggtcga gaggaatcga acgggcgcag tgacgcacgc gttgggcgcg
     1021 gcttcgcgtg acggatccac cgacgccgat atacattcca agatctaaaa aacgtcgggc
     1081 ttgctcgtag gatcctgaaa aagcgtgcaa cgctccttga actccggggt ggtttcgaag
     1141 caccgaatac agcaggtcga cggcctttct gctgtggagc gtgaccggaa gaccaaggcg
     1201 aaccgcgatc tgcagttgcg cttcgagcac ctggatttgg cgttcgacgg gatggcatgg
     1261 cgggtctccc cacaggtcca atccggcttc gccgactgcc gtggggtgat gagtctcgat
     1321 gagttgcaag acgttttcga gatcttgcgt ggcgaaccag ggatgaagtc caagggcaaa
     1381 aggcagcgag gggtgtcgtt catgcaacgc gcacgtgagt tgattggacg gctcgtggta
     1441 tccgcacgtg agcatgctca tgaccccacc atggcgagcg tctttcaacg cttgatcagg
     1501 ttcttcgagg tccgtgaggt gacaatggga gtcgaacatg agctaaatac cttttaatta
     1561 caacatgtta tcccattcgc gaaaatggcg aacgagcgtg gatgggccgg ggggcgcgca
     1621 ggaccacgaa gcatgaatgc atcttgtcgt ggcatgtcag gggccgacgg tcggagggtc
     1681 ctgtccggaa acggattgta cgagaaatcg ggtcacgtcc tgccgagcgg cttggtttcg
     1741 gaaagccacg aagtgaccgt cgtaatcatc gactccgtag cgacgggtca ggatagtcac
     1801 cgcggcccct tctaccgagg cattgcccgc atttccggta acgagaccca cgcctgccaa
     1861 ttcgttgtca tcattgggat tagcgacgac cgtggagggg tcgtcggcca cgcggccgaa
     1921 ccttgcggcg atgaggtagg tgagttgggt tttccctggg ctaaacgtat cccgttgacc
     1981 gaagggttga aaaacatggc gggcggtttg gggaaggggc ggcgtgatgg acatttgttt
     2041 tgcgtgggcg agtggatcgg cggggtcgat ccaggcttga agaaggctga gggctggatg
     2101 caaggagcct ccgggaagtt ggaagctttc gtccacgtcc ccgaggacga agggcagggc
     2161 tgcggcgatg ttgacgggac ttgttttgct gagcaatgac tccaacaggc ttgcgccatg
     2221 gcctgacaga acgacgccct tgatacccgt ggaatatggg attccgaggg caccctgggt
     2281 agctccttgg gagtggcccc agaaggagaa ccggtttgga tcgaagtgca gggcggaacc
     2341 ggtgggagaa gagggggcat cgatggagag catggataca aaacggagca aggagagctg
     2401 gtcggccgcg ccctgcattg cgttgtattt ggctgcaaag ggattggcga agttgaaaaa
     2461 cagattctcg gggctttcgg tggactcacc acggcgagga ccgtgttgta cctgatcgat
     2521 cccgatgacc gcgattccct cgttggccat ggtctcggcg attccgaggg aaacgtggga
     2581 tcggaagctt cctcccgtgc catgggagaa aacgacgagg ggccagcctt tttcgggcgc
     2641 ctgtatccct ttagggatag tcagcgacag gcagactttc tcctgtctcg cgatggtgaa
     2701 ggtggagccg gtaatttcga attgcccgcc ttgttcgaga tacggagcgg caccgtgttg
     2761 gaaaatgggt aaactcacca gggcgtggag ttcatcgaac ttggcgttgg gttgaccaca
     2821 agcccgatct tccgaggctt gaggacaagg agaaggagcg ttgccgcaac gaacccacga
     2881 cgatgcggtg ggagctggcg cggcgtccac ggaacgggcc atggtttccg cggcgtcacg
     2941 cacgggtccg acggtaaaca ccgcggcgtt gagcagcgtg cttttgtcga gccccttatc
     3001 cgcgaggtag tcgcgaaaaa ccttgtaatg gctgtgaggt tgaatgaggg cttgctcgac
     3061 gggaaccgag tcttcgagca tggcggagaa gtccggggag cgtttgacga agttgccgtc
     3121 gggccttttt atcgatgagg aaaggatgac agcatacgtg atgcctggtt gcagaggttc
     3181 gccaaggggt ggtcgaaggg ccagccaatt agggcatagg tacttggtcc tcatgccatt
     3241 ggcgagggaa aaccaaccga gggtatagcc ataatggggg gaggttggtg tgatgtcgac
     3301 ccattgaacg gcacctggat tgacaccgtt atcgatcgag ttccaatcca cgctgttgga
     3361 gaatcgaaac acgaccgtgg gataaatgcc aaatccgaca ttttcctttt cgatggcgcg
     3421 aaggtagggc tccaacgggt cgaaaccgag cagggaagcg cctggcgttg ggtgtcccgt
     3481 caggtctgga tgaccatcgc gagtacgaat atcgttggga aaaggaatcc gataaaagtc
     3541 accgtttccg gatagtcggg ggacttcgaa gtaggctacg gcgggctggc taaggtcatc
     3601 gctacacgtt tctccggccc aagaaggcat accgaaggaa ggggctcctg gaggcgtttt
     3661 cgcgcagacg tcatgaacac aggctagtcc accaaaacag tccgcgctgc tcacgcaagg
     3721 ttgccccacg tcgtgatcgc cttgctcgat gcaaaccaaa gcaaagtttt tgaatccgca
     3781 cttcaaccca tccctgcaat ccccatcgga tgtgcaagcc tctccaagct ggccgcttcc
     3841 agcggtcgca catcgaccgt gggcggtgca atacagccct tcctggcact cggcgctaat
     3901 cacacaagga ccgttttcgg gttgactctt cccaggcgca cattgcccat ggacgcactc
     3961 caatccggtt cggcatcggt tttgctcatc gcagggttga ccgactccgc tttgtgaacc
     4021 ctggggcggg gttatgtcac cagactcgct ttgggtgacg gggctaccgg aagagcagct
     4081 cgccaacgat gaccagcaga gcgcggagat gaatacaaag gaggcccgac gcagcattac
     4141 cccttaggat tcagcaagtc ataacgcgat gcaagccacg gcaaggaaag aagaagaata
     4201 cgggaaggaa aagtctgatg ggttgcgcga ccgtccttgg gacgagccat tatttagggg
     4261 gatcggagga aggcgatggc cgataagggg aaggaaactc cccgctgaat agcagctttc
     4321 gagcgttggt cacatacaac tttcggagca cgtcgagcgg aagaccaatg gcatccacct
     4381 tccatttgcc ctggcttggc gtgggatgat cgatttgggg catggaggtt tcgaagtacc
     4441 gccaatgcct ctcgtaaaac tctctggcgt ccgccacggt cggctcgcga tcggacacgg
     4501 agccaagttg catccctccg gtgggacctg cgatgaagtc cgaaccaaac aaaatgcggt
     4561 cttggtgctt gatgaacaat gcacgcactt ttgcggcggg atgccgtccg atttcgggaa
     4621 cccgcgccga tgtatcgacg aaaacattgg ggcaagcatc gagcaagcgg tccacgtagt
     4681 cgatgttttc aggatagttg ccgaaatgaa tgaggagaaa aaccgtatgg ggatgccgtc
     4741 gaagcacacg gtcctgggca gcgatgagtt cggcatggcg cgggtaatcg ccaccgtaaa
     4801 agctccagtc cggcgcgagt ttgagttcgt cgtagcgctc attatcaggg gtgaccgggt
     4861 cgaagaacgc cacgggatcg ccgatatgcc acgcaacgat cagcccgagt ttgccgcagg
     4921 cgtcgaaaat cggagcaagc agaggggcgt ctgcggcgat cagtctgcct tggctatcgc
     4981 gcacggccag tcccaaggcc ttgaaaatct tgatgcccac gatcccatca cgttttgcct
     5041 gttcgaggag cgcgacttgc tggtcgatga agtcgggctg attgacaccg cgccagtcaa
     5101 tgttggcaaa tgcccgcatt cgtcctcgaa gggcctg
//
LOCUS       DAOSOR010000436         5109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_16476_length_5109_cov_3.823704, whole genome shotgun sequence.
ACCESSION   DAOSOR010000436 DAOSOR010000000
VERSION     DAOSOR010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16476_length_5109_cov_3.823704"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="PK710_19045"
     CDS             complement(<1..1379)
                     /locus_tag="PK710_19045"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOT11883.1"
                     /translation="MTQSSESPAIRLVALGGLGEIGMNCLAIEAAGKLLVIDCGVSFP
                     HTDLGVDVYHPDFSYLERRRDRVVGIVLTHGHEDHIGGLPYLLRVLDVPVHGSCHALG
                     LASERLLERGFSPRQIRLMPFSVGQRFEAGPFSIEPVRVSHSIPEACALVIRHQRFTI
                     VHTGDFKFDPDPPDGHATDEERLRQIGDEGVSLLLSDSTNIDAEGMSGSERSVGRALS
                     DVISACETRVVVGLFSSNVHRLRMVGEIAQACGRKITLLGRSAQTQVRVATRCGLLNW
                     PSDLLIPPDLLGSVGQRQSLIIASGTQGEPMAALSRLSFQTHPALTLAPKDVLVLSSR
                     VIPGNDPAVSRMISNFIRQGVSVVHRVTNPSIHVSGHAHRDEQRRMIELLRPSVFVPV
                     HGTLHHLHRHAEFARSLGVPTVMAAENGEVLELSSGGIRVGDVVEVGKIATYNGDELL
                     PSVLKQREY"
     gene            complement(1596..4118)
                     /locus_tag="PK710_19050"
     CDS             complement(1596..4118)
                     /locus_tag="PK710_19050"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT11884.1"
                     /translation="MARKAVGVAIALALTAFGPAATAQGRIETVTTAPDSQQRALGVG
                     LMATPSGYRIFARICATSNCPVDEKSALSVQPPAGIGPTAPRLQSLRIGGGRWVVHVD
                     VSTATGRWQAIVAAPLSGVDPLVIWSGATPETPIDDRPRASLQVVTDATGNSQVLVGE
                     VREELQLCGRPTLLSPRVVHTDLSLRPVKLQRLSLAEREAAESVVAVGVEQVMEPLGR
                     LLVATGASSGHGSPSALSDGNLETPWSEGRGKEGRGEFVVFRAPSDVAIASLSLTIRP
                     RTAMVEQGAAPRTVYLAADGRLIRVTLPEDGWKHPGKSYSVTFAQPLETSCLAVVLDD
                     AFVPLAATDPRVTLSEVTAYSVFDGKETHASLARALGGNDKRAAAAASILLRGGDPAV
                     QAVVEHIHQLDASGRHHAMQVLDAAPCSVSSPVFASLLSSEVETERGHARDRIQRCGR
                     SAASALEQVLRHGAGCAPSYRNEAQCEKTMAAEKRHALDAGRLAAADELATVAPERVV
                     PWIAFQLGKMNRPTRRALRGYLARATSKPRGRNALALQLMNESLSVPARIDLLRVAKE
                     NLEGIRGPASVAFSRLATPNATLRTRTLLQDTAAQLARQNDEYALWYLRTSVTKDPEP
                     MVRTQAVTASRGIVEMQPWLMAALEDEQVRVREAAAQSLAGTPEAVPYLVRRLMLDPW
                     PMVRAAAARSLANSGPSSLADQELGRRLQDDAPLVRQWAATALGIRGAVSQTERLRAM
                     ADDSREKVTVRIVAVHALGELCDSKSVDLLTIFAARSTDPYSPEAASGLGAACVAALG
                     RLHPPDLARRLDPLISRPGVPAHIRSAAAEALSASNHCSSPR"
     gene            complement(4122..>5109)
                     /locus_tag="PK710_19055"
     CDS             complement(4122..>5109)
                     /locus_tag="PK710_19055"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOT11885.1"
                     /translation="DFALWKAATEDGYGWPSPWGKGRPGWHIECSAMSTKYLGFGFDI
                     HGGGMDLIFPHHENEIAQSEAAHPHQGPFARVWLHNGFVNVDKEKMSKSLGNFVTVRD
                     VFDRNDPEGFRYFLLTVQYRGPLTFDSEKLDDGRVIFPGVDEAERRMDYLYSTCHRLR
                     TLAEGAEPSAAKEPADLAAIRKVMTSARDKVSAALDDDLNTPVALAVLAEIAKYSNDL
                     CDLAIKRRKDASWVVCARQLARQALEVLDACTQVLGLLRTPHEQYVERTRERRLRLRN
                     LSQAQVEAKISERAQARKNKDFERADALRAQLEDWGIEMADGVEGTTWKVLV"
BASE COUNT         1135 a         1646 c         1350 g          978 t
ORIGIN      
        1 aaatactccc gctgcttcaa tacgctggga agaagctcgt caccattgta ggtggcaatc
       61 ttcccaacct ccaccacatc acctacgcga atcccacccg aagatagctc gaggacttca
      121 ccattttccg cagccatcac cgtgggtacc ccgagcgaac gagcgaactc cgcgtgacga
      181 tgcaagtggt ggagtgtccc atgcaccggc acaaatacgc taggacgaag caattcgatc
      241 atgcgccgct gctcgtctcg atgggcatgt cccgatacat ggatcgaagg gttcgtgacg
      301 cggtggacga cactgactcc ctgacgtatg aagttgctga tcattcgaga gacagcggga
      361 tcgttgcccg gaatgacacg gctcgacagc accaacacat ccttgggtgc gagcgtgagc
      421 gcgggatggg tctgaaagga tagtcgcgaa agagcagcca tcggctcgcc ttgggtgccg
      481 ctggcaatga tgagcgattg acgctgcccc acactgccca acaggtccgg aggaatcagc
      541 aaatcactgg gccaattcaa caatccacaa cgcgtggcta cccgcacctg cgtttgcgcg
      601 cttcttccaa gcaacgtgat ttttcgaccg caagcttgcg ctatctcccc caccatgcgc
      661 aaacgatgca cgttcgacga aaacaatccc accaccacac gcgtttcaca ggccgaaatc
      721 acgtccgaca aggcccggcc caccgaccgc tcactgccgc tcatcccctc ggcatcgata
      781 ttcgtgctgt ccgacaataa caacgatacc ccttcgtccc caatctgacg caaacgctcc
      841 tcatccgtgg catgaccatc cggaggatcg ggatcgaact tgaaatcgcc ggtgtgaacg
      901 atcgtgaagc gttgatgacg gatgaccaga gcgcaagctt ccggaatgga atggctgacc
      961 cgaacaggct cgatcgaaaa agggcccgcc tcgaatcttt gccccaccga aaatggcatc
     1021 aaacgaattt gtcgcggcga aaaacctcgc tccagcaacc gctcgctcgc caatccaagc
     1081 gcatgacaag aaccatggac cggtacgtca agcacccgta acaagtacgg caaaccgccg
     1141 atatgatcct catggccgtg cgtgaggacg atccccacga cccgatcccg ccgtcgctcc
     1201 aaatacgaaa agtcagggtg atacacatcc accccgagat cggtgtgggg aaaggaaacg
     1261 ccacaatcga tgacgagcag cttgccagca gcttcgatgg caagacaatt catcccgatt
     1321 tcgcctagtc ctccaagggc aacaagacga atggctgggg attcggaact ttgggtcacg
     1381 atcaggtggc ctttggggtt gaaacgaaat cgtatggccc gatctaagag gacgccgtgc
     1441 ttcaggtcaa ccaggtcctt gagcacaccc ccgaatcacg ggcgattcaa cgagatgtcc
     1501 acacgccctt gcccctcttt cccttccctt cgtctttcgg atggcaaaaa tatcgtgtaa
     1561 tattcaatag ttgtcgttgt ggtggaaggc gggggtcaac gcgggctcga acaatgattg
     1621 gaagccgaca acgcctccgc tgccgccgat cgaatatgcg cgggaactcc agggcgggag
     1681 ataagggggt cgagacgtcg agccaagtca ggtggatgca accgaccaag cgcggcaaca
     1741 caggcggctc caagaccact cgcggcttca ggagaataag ggtcagtcga gcgagctgca
     1801 aaaatcgtca ataaatccac tgatttacta tcacacagct cgccaagggc atgaaccgcc
     1861 acgatacgaa ccgtcacctt ctctcggctg tcgtcagcca tggctcgcaa tctttccgtt
     1921 tgagaaacgg caccacgaat ccccaaggcc gtggccgccc actgacgaac caaaggcgcg
     1981 tcgtcttgca accgacgacc gagttcctgg tctgccaagg acgatggacc ggagttggct
     2041 aacgaacgcg cggcagccgc tcgaaccatc ggccagggat ccagcatgag acgacgaacg
     2101 agatagggca cggcctctgg cgtgccggcc aggctttgcg ccgccgcttc gcgcacccgc
     2161 acctgttcgt cttccaaggc tgccatgagc cacggttgca tctcgacaat cccccttgac
     2221 gccgtgacgg cttgcgtgcg gaccatgggt tccggatctt tggtcacgct cgttcgcaaa
     2281 taccatagcg cgtattcatc gttttgtcgg gcaagctgag ccgctgtgtc ttgcaacagg
     2341 gttcgcgttc ggagcgttgc gtttggagtg gccaatcgtg agaacgctac acttgcaggt
     2401 cctcgaattc cttcgagatt ctccttggcc acgcgcagca aatcgatcct cgccgggacc
     2461 gacaacgact cattcatgag ttgcagagcc aacgcgtttc gaccgcgagg cttggaagtc
     2521 gctcgcgcca aataaccccg taacgcgcgt cgtgtcgggc gattcatctt cccaagttga
     2581 aaggcaatcc acggtacgac ccgctccgga gctaccgtgg ccaactcatc ggccgcggcc
     2641 agccgccccg catccaacgc atgacgcttc tcggccgcca tcgtcttttc acattgcgcc
     2701 tcattccgat acgagggcgc gcatccggcc ccatgccgga gcacttgctc caaagcgctg
     2761 gcagcacttc gcccacagcg ttggatgcgg tctcgagcat gcccacgctc cgtttcgact
     2821 tcactcgaaa gcaaagatgc gaatacaggg ctcgaaaccg agcaaggggc ggcatcgagt
     2881 acctgcatcg catgatgccg acccgaagca tcgagttgat ggatatgttc caccaccgcc
     2941 tgtaccgcgg gatctccccc ccttagcaat atcgacgccg ccgctgccgc ccgcttgtca
     3001 ttcccaccaa gcgctcgtgc gagacttgca tgcgtctcct tgccatcgaa aaccgaatac
     3061 gccgtgacct ccgataacgt gacacgagga tccgtcgccg ccaaaggcac aaatgcatcg
     3121 tcgagaacga cagccaaaca cgaagtttcc agcggttgcg caaacgtgac cgaatagctt
     3181 tttcccggat gcttccaccc atcctccggc aacgtcacgc gaatcaatcg tccgtcagca
     3241 gcaagataga ccgttcgcgg cgccgccccc tgctccacca tcgccgtccg cggacgaatg
     3301 gtaagcgaaa gactcgcgat ggctacatcg gaaggagcac gaaacacaac gaactcccct
     3361 cgcccttctt tgccgcggcc ttcgctccat ggcgtttcaa gattcccatc cgataacgca
     3421 ctcggggaac catgcccact cgacgcgccc gtcgccacca gcagccgccc taaaggctcc
     3481 atcacctgct cgacaccgac agccaccacc gattccgccg cctcacgctc cgctagggaa
     3541 agacgctgga gtttgaccgg ccgaaggctc aaatccgtgt gaaccacccg tggagacaac
     3601 agcgtgggcc tgccacataa ttgaagctcc tcacggactt ctcccacgag aacctgactg
     3661 tttcccgttg cgtccgtcac cacctgcaac gacgcgcgag ggcgatcgtc gatgggggtc
     3721 tctggcgtgg caccactcca tatcacgagc gggtccactc cggataaggg cgccgccaca
     3781 atggcctgcc atcgacccgt tgcggtgctc acatcgacat gaaccaccca tcgtcccccc
     3841 ccaatacgaa gcgattgcag acggggcgcc gtggggccaa ttcccgcggg aggttgcacc
     3901 gaaagcgctg atttttcgtc cacgggacag ttcgatgtgg cgcagatgcg agcaaaaatc
     3961 cgataaccgc tcggcgttgc catgagtccc acgccaagcg cacgctgttg cgaatcagga
     4021 gccgtggtga cggtctcgat cctgccttgc gctgtcgccg caggaccgaa cgcggtcaac
     4081 gccaacgcga tggcgacccc aaccgccttt cgagccatcg attacaccaa aaccttccat
     4141 gtggtgccct ccacgccatc cgccatctcg atgccccaat cttcgagttg agcacgaagg
     4201 gcgtcggctc gctcgaaatc cttatttttt ctggcttgcg cgcgctcgct tatcttggcc
     4261 tcgacctgag cctgcgacaa gttgcgaagt ctcaaacgac gctctcgcgt tcgctccacg
     4321 tattgctcat gaggcgtacg caacaaccca agcacttgcg tgcatgcgtc cagaacctcg
     4381 agtgcttgtc ttgctagttg cctggcgcaa accacccagg acgcatcttt gcgccgtttg
     4441 atggccaaat cgcataagtc attggaatat ttggcgattt ctgcaagaac cgcaagcgct
     4501 accggcgtgt tgagatcgtc gtcgagcgcc gcgctcacct tgtctctggc cgacgtcatc
     4561 accttgcgaa tggcggccaa gtctgctggt tctttggcag cgctaggctc tgctccctcg
     4621 gcaagggtcc gcagtcgatg acaggtggaa tacaaataat ccatccgacg ttctgcttca
     4681 tcgacgccgg ggaaaatcac gcggccgtcg tccagctttt ccgaatcgaa ggtaagcggg
     4741 ccgcgatact gcacggtgag cagaaagtag cgaaacccct ccggatcgtt ccgatcgaat
     4801 acgtcgcgta ccgtcacgaa attgcccaat gacttggaca ttttttcctt gtccacattg
     4861 acgaaaccat tgtggagcca gacgcgggcg aagggtccct gatgcgggtg ggcagcttcg
     4921 ctttgcgcga tctcgttttc gtgatgggga aaaatgaggt ccatacctcc cccatgaata
     4981 tcgaatccga agccgagata cttggtgctc atcgccgagc attcgatatg ccatccgggc
     5041 cggccttttc cccatggact cggccaacca tagccgtctt ccgtcgcggc tttccatagg
     5101 gcaaaatcc
//
LOCUS       DAOSOR010000437         5099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_16527_length_5099_cov_3.930611, whole genome shotgun sequence.
ACCESSION   DAOSOR010000437 DAOSOR010000000
VERSION     DAOSOR010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16527_length_5099_cov_3.930611"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(762..1646)
                     /locus_tag="PK710_19060"
     CDS             complement(762..1646)
                     /locus_tag="PK710_19060"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HOT11886.1"
                     /translation="MECINRGIFDNPVTDGWETFPTSFMIQDSSLSMDPDRHLTLQNA
                     YNFRDIGGLRTHDGRRVQKGLLYRSDALSRLASSDQEAIAGLGLKTVVDLRTESERKS
                     APDRLGDDRRIRVVHHPMRDPAVPESRWRMMIELIVRGRDIDMGALLQRQYVAFAFDC
                     GAALGAVVRLVAERAHLPLLIHCTAGKDRTGLVAAVILRMLGVSMDDVLDDHIATNEL
                     LRPAFPHFEKTLRRMSMGRLTPEQIRPLLEARAEDLEHVFRTIDERHGSIEGWLEQVC
                     GIGAETTERIRQVLLTEP"
     gene            1650..3089
                     /locus_tag="PK710_19065"
     CDS             1650..3089
                     /locus_tag="PK710_19065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11887.1"
                     /translation="MRRYLVIRSFCALAGFLLACGTSEDSSSWDENRDIDRDAASSSD
                     NRNEETEPSTDGDGDGDAALDVGTDDAVDTEADALDAPLDAQDDSDDALDGTVEDAVD
                     ATSDSAVDSGNTPPDPPEVTVGTVTETKLFNITGTSTSYTTVQGGGSDGTHAYVVLLH
                     KKAGGIDISQLVKYRISDGQRVATASFDAPGDVTNLLGHGNDATYNPDTKRLIIPAWT
                     NDNSKQPADNGKHLRIIDPATLKIVDTKTVDINVTNLCYVNGGYLMFSGGKFRTYDAN
                     FSLTKTVAFDIKAVEDDYAPTGDRIGQGIDCDADYVYITRWYPGPKSNAIYVANHQGQ
                     LVGVYYSGGSEGEHLMHVSGERMLHGMNNIGSVGGELRRIEKLFFVVSYVAEGGSGSM
                     SKTRVLYGRKTPLRKNAFTVSGKQFSGWAAERESDGKWRYQSGDGKESGWYAKGKQPS
                     GWSYYIYKDKAIVYGSAPFGLVKMHAQWK"
     gene            3181..5067
                     /locus_tag="PK710_19070"
     CDS             3181..5067
                     /locus_tag="PK710_19070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11888.1"
                     /translation="MDPKAVELQAAVIRVLQDDRLDDRELLKEIEQLAASDPFRFPGF
                     SHLWAPELYRRNRFIFREFILYRSNAFGDHGYYDDRGNLFNPWSATETRKTLEELLDQ
                     CGKDNEVRLFRRLYEESIGGSWRGAETWRTDLKNAFLSAADDRQRRVALEKYDLPSAW
                     LDEPTALALYQRSPSLCSAFILAHAPTNLSSLWGKPRGPWHQLRQLATEKGDIDFEQK
                     LFRLQATEQQWAERVRSISPIASPQQRVDSLDRIHPPTTPRNATQVYYELLTTHGSDV
                     LPYVMKHLDLRGHAYWSGNLKGLDKLIQLAWDNGWLALWAGLLRNSDEASYNRAITNL
                     LDDQGSLGEYETKRRLLLLAGLGQEMNHPGFGLALALPLTEKTAMAMYRRFPDLLRTS
                     FRHHCRPDWAHTYESLVRAAIEAPDETLVDWFASRVITMVIHGASHKHAAIVRLLVQH
                     YETLQPQPETLARRCGAVLSAIPAGVIGNYDYLLRNNALAQWMFQQKLVAYLHDPRVV
                     RDFLESPQIHVNQLGFHILSLDDPAVAQLSEHNLDLLCAALLRKLHRRTRLLAIDALL
                     QAVRTERAARVVLPKARAALRLPDKRYPKENLIALIATILHRHPSLRSEREQPPIFDH
                     RMQP"
BASE COUNT         1167 a         1442 c         1407 g         1083 t
ORIGIN      
        1 gacatcgcca tgccgtcaca gtccccgggg gcaccccttt gcgttttgca gcctcgagtg
       61 gccccacctc ttgtgccaaa cgcagaatct cggcgcgttg ttgcgcggta cactttgcgg
      121 ccatcttccc atcctccaag acggcatcct gaacacccca tgggcccggt gatattgccg
      181 tcgaattgga tggtcggtgt atgacaaggg caagtagcca ccagaaagaa ctgggcgtgc
      241 gtcgatccca actttccgtt gacgagtgaa tatattgccg cagacggatt tcacccgagc
      301 gcggccgcgt attccctttg ggcggagaaa atggcatccg tgatccgcca caaatggcag
      361 cagaatagca atagccatgg gtccaaacgg gtagatgggt agacgggttc atgggcctgt
      421 gctggctgcc ggtgtggcag atgagtttcc taggccgatg ataaggcttt tgcagcgttg
      481 catgaacttt cgaggtcatg acaacgaccg tatatcgtag gaccctggtg ttttctcggc
      541 atgacaagag cgaaacatga acagggcagt atactagcgg tttgtggtaa acctgttggg
      601 aaaaaagact acccattaac gtgactacag gggataaagc gaagggatga gcaaacgaat
      661 aagtgattga taccattcag tttttcggag agggagactg atggtgagcg gctggcaggt
      721 gataccgcga gcgcagtccc ccgtcgaacg ctccaccacc cctacggttc cgtaagcagc
      781 acttgtcgaa ttcgctcggt tgtctcagcg ccaattccgc aaacctgctc taaccagcct
      841 tcgatcgaac cgtgtctttc gtcgattgtc cgaaacacat gctccaaatc ctccgcgcgc
      901 gcttcgagca gaggtcgtat ctgctctggc gtcagccgtc ccatactcat ccgacgaagc
      961 gttttctcga agtgtggaaa agcaggacgc aataactcgt tggtcgcaat atggtcgtca
     1021 agcacgtcat ccatcgacac gccgagcatt cgtaagatca cggcagccac aaggcccgtg
     1081 cgatcttttc cggccgtgca atgaatcaac aggggcaagt gggctcgctc ggcgacgagt
     1141 cgaaccacgg cgcctagggc tgcgccgcaa tcgaaggcaa acgctacgta ttggcgttgc
     1201 aacaacgcgc ccatatcgat gtcccgaccg cgtacgataa gctcgatcat cattcgccaa
     1261 cgagattccg gtaccgctgg gtcccgcatg ggatgatgaa ccacacgaat gcgccgatcg
     1321 tccccaagac ggtctggagc tgattttcgc tcactttccg tgcgaagatc gacaacggtc
     1381 ttcaacccaa gacctgcaat agcctcctga tccgacgagg ccagtcgaga aagggcgtcc
     1441 gatcgataca gcaagccctt ctgaactcgt cgtccatcgt gggtccgcaa ccctccaatg
     1501 tcgcggaaat tataagcgtt ttgaagagtc agatgtcggt ccggatccat cgatagtgag
     1561 ctgtcttgta tcatgaaaga cgtcggaaag gtttcccatc catcggtcac ggggttgtcg
     1621 aatatccctc ggttgataca ctccatctca tgcgtcgata cctcgtcatt cgctctttct
     1681 gcgcgttggc tggatttcta ctcgcctgcg gcacttcgga ggacagcagt tcgtgggatg
     1741 aaaaccgtga tatcgatcgc gacgccgcat catcatcgga caaccgaaac gaggagacgg
     1801 aaccttccac ggacggcgat ggggatgggg acgctgcgct agacgtcgga acagatgacg
     1861 ccgtcgacac ggaagccgat gcgctcgatg cgccgttaga tgcccaagac gatagcgatg
     1921 atgcgctcga tggtaccgtg gaagatgctg tggatgcgac gtcagatagc gccgtggaca
     1981 gtggcaatac gccacccgat ccgcccgaag tgaccgtggg tacggtcacc gaaaccaagc
     2041 tgttcaacat cacgggcaca tcgacttcgt atacgacggt tcaaggtggc ggatccgacg
     2101 gcacgcatgc ttatgtggtt cttctgcaca aaaaagctgg aggaatcgat atttcacagc
     2161 tcgtcaagta ccggatttct gatgggcaac gagttgcgac cgcttcgttc gacgctcccg
     2221 gagatgtgac caacttgctc gggcacggca acgacgccac ctacaaccct gacacgaaaa
     2281 ggctcatcat accggcctgg acgaatgata attcgaaaca accggcggat aacggcaagc
     2341 atcttcgcat catcgacccg gccaccttga agatcgtcga caccaaaacc gtggacatca
     2401 atgtcaccaa cctctgttat gtcaacggcg gctacttgat gttctccggg ggcaagttcc
     2461 gcacctatga tgcgaatttt tcgctcacga aaacggtcgc tttcgatatc aaggctgtcg
     2521 aggacgacta cgctccgaca ggagatcgaa tcggccaggg aatcgattgt gatgccgatt
     2581 atgtgtacat cacgcggtgg tatccaggac ccaaatccaa tgctatctat gtcgccaatc
     2641 atcagggaca actggtcggc gtgtattatt cgggcggttc cgaaggggag cacctgatgc
     2701 atgtatcggg cgaacggatg ctgcacggaa tgaacaatat cggctcggtg ggcggcgagc
     2761 tgcgacgcat cgagaagctg tttttcgtcg tcagttatgt ggcggagggc gggtccggtt
     2821 cgatgtcgaa aacccgtgtt ctttatggaa gaaagacacc tttgcgcaaa aacgcgttca
     2881 ccgtttcggg caagcagttt tcgggttggg ccgcggagcg cgaaagcgat ggcaaatggc
     2941 gctaccaaag cggtgatggc aaagagtcgg gctggtacgc gaagggcaaa caaccgagcg
     3001 ggtggtccta ctatatctac aaggacaagg cgattgttta tggttccgca ccttttggat
     3061 tggtgaaaat gcatgcgcaa tggaaataga gggaagaacc aggcgcggcc tttcgacatg
     3121 ggagacgaca cacaacaatt cggctcaccc tggaccaccc tgcgataacg tgagcgatgc
     3181 atggacccaa aagcggtaga gcttcaagct gcggtcatcc gcgtccttca ggacgatcgg
     3241 ctcgatgatc gagaacttct caaagaaatc gagcagcttg cggcctcgga cccctttcga
     3301 ttccccggct tcagtcatct gtgggccccc gagctgtacc gacgaaatcg ctttatattt
     3361 cgagagttta tactctatcg gagcaatgcc ttcggcgacc acggctacta cgatgacagg
     3421 ggcaacttgt tcaatccctg gagcgcgacc gaaacgagaa aaacactcga agagctgctc
     3481 gatcaatgcg gaaaagacaa cgaggtccgg ctgtttcgcc gtctttatga ggaaagcatc
     3541 ggtgggtcgt ggcggggagc cgaaacgtgg cgaaccgatc tcaagaacgc ttttttgagc
     3601 gcagccgatg atcgacaacg tcgcgtggcg ctcgaaaaat acgatctccc atcggcatgg
     3661 ctggatgaac cgaccgccct ggctctttat caacgatcac cttcgctgtg ttcggcgttc
     3721 atcctggctc acgctccgac gaatctatcg agtctgtggg gcaaaccacg cggcccgtgg
     3781 catcagttgc gtcaacttgc caccgaaaag ggcgatatcg acttcgaaca aaagctattt
     3841 cgtcttcagg ctaccgagca gcaatgggcc gagcgtgttc gatccatcag ccccatcgcc
     3901 tcaccgcagc agcgggtcga ttcgctggac aggattcatc cgcccacgac accccgcaac
     3961 gctactcagg tttattatga gcttctcacc acgcacggaa gcgatgtgtt gccttatgtc
     4021 atgaagcacc tcgacttgcg gggacatgct tattggtcag gcaatttgaa aggattggac
     4081 aagttgatcc aactcgcctg ggacaacggg tggctggctc tatgggcggg tttgcttcgt
     4141 aactcggacg aagcctctta caatcgcgcg atcaccaatc tattggacga tcaagggagc
     4201 ttgggagaat acgagaccaa gcggcgcctg ctgcttcttg cgggccttgg gcaagagatg
     4261 aaccatccgg ggtttggatt ggcgctggcg ctaccgctca ccgagaaaac ggcgatggcg
     4321 atgtatcgtc gttttcccga cctgcttcgc acttcatttc gacaccattg tcggccagac
     4381 tgggcccaca cctacgaatc cctcgttcgg gctgccatcg aggccccaga cgagaccctc
     4441 gtggactggt tcgctagccg tgtgatcacc atggtcatcc atggtgcatc ccacaagcat
     4501 gcggccatcg tgaggctttt ggtccagcat tacgagacgc tgcagcctca gccggagaca
     4561 ttggcgcgac gatgtggcgc ggtgttatcg gccattccgg cgggcgtcat cggcaactac
     4621 gattatcttc ttcgaaacaa cgctctcgcg caatggatgt tccagcaaaa actcgttgcg
     4681 tatctgcacg accctcgagt cgtgcgcgat tttctcgagt caccgcaaat tcatgtcaac
     4741 cagcttggat ttcacatttt atccctcgat gatcccgctg ttgcgcagtt gtctgagcac
     4801 aacctcgacc tgctctgcgc cgcgctgctg cgcaagctac atcgtcgaac gcgcctgctc
     4861 gcgatcgatg cactcctcca agcggtgcga acggaacgag cggcacgcgt ggtattaccc
     4921 aaagcacggg ccgccctgcg attacccgac aagagatacc ccaaagagaa tctcattgcc
     4981 ttgatcgcca ccatcttgca ccggcatccc tccttgcgct ctgagcgcga gcagcccccc
     5041 atcttcgacc accgaatgca accatgatcc cgctagcagt ttcgtatgct tctccaagc
//
LOCUS       DAOSOR010000438         5097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_16535_length_5097_cov_4.240777, whole genome shotgun sequence.
ACCESSION   DAOSOR010000438 DAOSOR010000000
VERSION     DAOSOR010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16535_length_5097_cov_4.240777"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PK710_19075"
     CDS             complement(<1..676)
                     /locus_tag="PK710_19075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT11889.1"
                     /translation="MNESSRPVLAIRGLSTEVQTRRGVFRAVDDVSFTVPAAGKVALV
                     GESGSGKTMTALSVLRLTDPGVRIVAGVVEFKGQDLLLLRENQMRHLRGSELAMVFQE
                     PMTSLNPMLSVGYQIVETLRAHQKISRKQAKQRAVELLHRVGIPSPSTRVDAYPHQLS
                     GGMRQRVMIAIAMGCRPSLMIADEPTTALDVTIQGQILDLFEQIREQDGTATWLITHD
                     LGVVAGF"
     gene            complement(673..1353)
                     /locus_tag="PK710_19080"
     CDS             complement(673..1353)
                     /locus_tag="PK710_19080"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HOT11890.1"
                     /translation="MRLAFSPACWTAVVAVGVGIAGAGCAGKPIPCVSSAACGPDTVC
                     VAGRCREGKDAPVPASAQRLVLEPVAMAVVSSSGTHERNTSIVSFGKASWGDVVLLLR
                     FAAPFGETTQILSAHVVLEPSVGFVPGPQPVRLQVARIVEPWSAAQASWATLPQLRVV
                     NTTFWASTWGERPLRIDVTEQVQRWREHRRDEQGLAIVSSPQNEIGETYSLGLLQGRG
                     PRLDVYLR"
     gene            complement(1837..2631)
                     /locus_tag="PK710_19085"
     CDS             complement(1837..2631)
                     /locus_tag="PK710_19085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOT11891.1"
                     /translation="MSDLEFVKVERDGYVALVTIHRPDKLNALSAAVVRDLMMAFHQL
                     VFPGDGERPRVVILTGSGEKAFVAGADIGEMATMTAAQAKAFADAGHRLGALMESAPF
                     VVICAVNGFALGGGTELALCGDFIYASDKARFGQPEVKLGVIPGFGGTQRLARRVGLG
                     MARELVYSGRLIDAAEALRIGLVNRVVPHADLMSKVREVAQEIAARGPVAVAAAKRCM
                     LRGYDQDLVNANELEAAEFAVLFGTADQAEGMKAFLEKREPSFLGR"
     gene            complement(2646..3539)
                     /locus_tag="PK710_19090"
     CDS             complement(2646..3539)
                     /locus_tag="PK710_19090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOT11892.1"
                     /translation="MNASDVKVFGVIGAGQMGSGIAQVAAAHGFKVLLLDVNRALVDN
                     AKGKIGAILQKQVDKGKMEASARERLLDHIEPCSEISVLQQADIVVEAATENTEIKLK
                     LFRSADEAMRQGAILASNTSSISITKLAACTGRPESVIGMHFMNPVPLMKLIELVRGL
                     QTSDQTYETVKALSEKLGKTVITSNDEPGFIVNRMLIPFLNEACFALQSRLGSVQDID
                     MGAKLGLNHPMGPLELADLVGLDTLLFIADVLHKEFGDDKYRASTLLRNMVAAGWLGR
                     KTGIGFYCYEGSDKKPNPRVL"
     gene            3709..5043
                     /locus_tag="PK710_19095"
     CDS             3709..5043
                     /locus_tag="PK710_19095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT11893.1"
                     /translation="MGLRLHPLVFFLLVSTSAWAQTDSDRATARLLAEEGIALQQQGK
                     YEEALDKLTRAQQIFNAPTHLLLIAQCQVAVGKLVEGAETYRQLLRKPLTEQAPQAFV
                     RAKETAARELAELEPRIPKIRILIHPDGIEQLHIRIDETAVPAAIVGIDRVENPGKHI
                     IQVSAPGFQTAQVQFQLQESQILPVEVTLEADTPSKPAPAPTTQVPHSYTVASTPEAN
                     APSSHSTDLLMSIRLQWTAPNGELGETHFNGSWIDPGAVRDSFHAGGGLELQADLLFG
                     NWAVGLLFGVDDHGQPSGSHEAVKSLYPTIESVQTTKSFSAFGGISGQWHSCQRCLGF
                     VGETAIIMRQMVQTFEIQPQSGPPDPCSLSWERDVPTVRLLAGLQIPVGSDFFVTPYL
                     SFAGGSTSVTRLEPSDDAPRCPSNKIESSETTGGSITFFSVGVAGTYALALD"
BASE COUNT         1133 a         1618 c         1304 g         1042 t
ORIGIN      
        1 caaacccggc caccacgcca agatcgtgcg tgatgagcca tgtcgccgtg ccatcttgct
       61 ctcgtatctg ctcgaataag tccagaatct gcccttgaat ggtcacatcc aacgcggtgg
      121 ttggctcatc ggcaatcatc aacgaaggtc tgcatcccat cgctatcgcg atcatcacgc
      181 gctgtcgcat ccctcccgat aactgatgcg gataggcatc gacccgcgtg gacggcgaag
      241 ggattcccac acgatgaagc aattccaccg ctcgctgttt tgcctgcttt ctcgatatct
      301 tttgatgcgc ccgcagcgtc tccacgatct ggtagccgac cgacaacatc ggattgagcg
      361 atgtcatcgg ctcctgaaaa accatcgcaa gctcactgcc tcgcagatgc cgcatctggt
      421 tttcacggag caacagcaag tcctgtcctt tgaattccac cacccccgcc acgattcgta
      481 cgcccggatc cgtcagccgc aacaccgaca aagccgtcat cgtcttgcca ctgccagact
      541 cgccgaccag cgccaccttc ccagccgcgg gaacggtaaa cgaaacgtcg tcgaccgcac
      601 gaaagacccc tcgacgagtc tgcacctcgg tgctcaaccc acgaatggca aggacaggac
      661 gagaagattc attcatcgca agtaaacgtc gagcctgggc ccacgacctt gcaaaagacc
      721 aagggagtag gtttctccga tttcattctg cggggaagac acaatcgcca atccctgctc
      781 gtctcgtcga tgctctcgcc aacgctgcac ttgctcggtc acatcgatac gcaaagggcg
      841 ctcgccccag gtggaagccc agaatgtggt attcaccacg cgaagctggg gcaaagtcgc
      901 ccacgaggct tgcgcggccg accagggttc cacgattcgc gccacttgca gacgaaccgg
      961 ttgcggtcca ggaacgaatc cgacggaagg ctccaaaacc acatgcgcag atagaatctg
     1021 ggtcgtctct ccaaagggcg cggcaaaccg cagcagcaat acgacatccc cccacgacgc
     1081 tttgccgaaa gacacaatcg acgtgttgcg ctcatgagtc ccagaagacg atacgaccgc
     1141 catcgccacg ggttccagca cgagacgctg agcactggcc ggcacaggcg cgtctttgcc
     1201 ttcgcggcaa cggccagcca cacagaccgt atccggcccg catgccgccg atgacacgca
     1261 gggaatcggt tttccggcac aacccgcacc ggcgatccct acacccaccg cgactaccgc
     1321 ggtccaacag gcaggcgaaa aagctaacct catggctcgc ggcttcttat ggctttgcga
     1381 gagcgccgtt gcaacaagat aggatgacca accttccaaa acgtccgtcg aatcatgacg
     1441 ataccaagcc tatttttcga tatccgtggc cttcttcgac gagccaggcc ctccaacaac
     1501 tcaccccaaa cggctgcctg ttgcctgttc caccctcttt cgactccaac ccatcgcagg
     1561 gcttcgctct tcgacaccgc cacggagaat catgatccca tccctgtgta tccgtatacg
     1621 gatttaatca tatcatggcc gaatcgaaag gctacccatg ggatctggca tcgatttatc
     1681 cgtggaaatt gaatagtttc agttgaaatt aaataaaagt atcatccatc caagaccatg
     1741 ggaagagcag aagattccga agaataaccg tatagggata tattggattc gaagctgggg
     1801 gaacggaagg gtgccgcgga aagagcgtac gattcgctat cgtccgagga acgacggctc
     1861 gcgtttttcg agaaaggctt tcatgccttc ggcttgatcc gctgttccaa aaagaactgc
     1921 aaattcagca gcttccaatt cattggcgtt gaccaagtcc tggtcgtagc cgcgcagcat
     1981 gcaccgtttt gctgccgcga cggccacggg accacgcgcg gcgatttctt gcgccacctc
     2041 gcgcaccttc gacatcaagt ctgcatgagg caccacgcga ttgacgagtc cgatgcgcag
     2101 cgcttcagca gcatcgatca agcgcccgga atacacgagt tcccgtgcca taccgagtcc
     2161 cactcgtcga gccagacgct gggttcctcc gaagcccgga atgacgccga gtttcacctc
     2221 cggttgtccg aaccgtgctt tgtccgaggc gtagatgaag tcaccacaaa gggccaattc
     2281 ggttcctccg cccagggcga acccattgac cgcacagatc acgacaaacg gagcggactc
     2341 catcaacgct cccaaccgat gccccgcgtc ggcaaaagcc ttggcttgcg cggcggtcat
     2401 ggttgccatc tccccgatat ccgctccggc cacgaaagct ttttcgcccg aaccggtcag
     2461 gatgacaacc cgaggacgct cgccatcgcc agggaatacg agttgatgaa acgccatcat
     2521 caggtcccgg accaccgcgg cgctcaaggc attgagttta tcaggacggt gaatcgtaac
     2581 gagtgcgacg tatccatcgc gttcgacttt cacaaattcg agatcgctca tggtcatgct
     2641 cctgctcaaa gaacgcgcgg attgggcttt ttgtccgagc cttcatagca atagaaaccg
     2701 atgcccgttt ttcgtccaag ccatcccgcc gccaccatgt ttcgaagcag cgtcgaagcc
     2761 cggtacttgt catcgccaaa ctccttgtgc agcacgtccg cgatgaaaag caacgtgtcc
     2821 aaccctacca aatcggccaa ctccagcggc cccatcggat gattcaggcc aagcttggcc
     2881 cccatgtcga tgtcctggac cgaccccaac ctcgattgaa gagcgaaaca cgcttcgttg
     2941 aggaaaggaa tgagcatccg attgacaatg aaaccaggtt catcattcga ggtaatcacc
     3001 gtcttaccga gtttttcgga cagagctttg accgtttcat aggtctgatc gctcgtttgc
     3061 agtccgcgca ccaactcgat gagtttcatc aaggggacgg gattcatgaa gtgcatgccg
     3121 atgacggatt cgggacgacc cgtacaggca gcgagtttgg tgatggaaat ggatgaggta
     3181 ttggaggcaa ggatggctcc ttgtcgcatg gcttcatcgg cagaacggaa gagtttgagt
     3241 ttgatttcgg tattttccgt ggcggcttcc acgacgatat cggcctgttg cagcacgctg
     3301 atttcactgc aaggttcgat atggtcgagc aggcgttcgc gcgcgcttgc ctccatcttg
     3361 cctttgtcca cttgtttttg gaggatcgcc ccaattttgc ctttggcgtt gtcgaccaag
     3421 gcgcggttca catccagcaa aagaactttg aagccgtggg ccgctgccac ttgcgcgatg
     3481 cccgagccca tctgcccggc accgatcacg ccaaacacct tcacgtcgga tgcattcatc
     3541 ggttttcctc atgcgtcgtt ggggatggag acggtccctg tttgcgttca ccatgccagt
     3601 ccacgaatga accgacaccc ttgcctttcg gttaggcggg ctggactggt gggtcaatgg
     3661 tgtcttggaa agtacctcgt acgggccggt tgatgtacat tggtcgcaat gggattacgg
     3721 cttcacccgc ttgtgttttt cctgctcgtc tccacctccg cttgggcgca aacggattcg
     3781 gacagggcca ccgcacggct ccttgcggaa gaaggaatcg ctcttcaaca gcaggggaaa
     3841 tatgaagagg cactcgacaa actcacgcgc gcccagcaga ttttcaacgc tccgacccat
     3901 ttgttgctca tcgcccaatg ccaagtcgct gtcggaaaac tcgtcgaagg cgccgaaacc
     3961 tatcggcaac tgctacggaa acccttgacg gagcaggccc cccaagcctt tgttcgcgcc
     4021 aaggaaactg ccgcgcgcga gttggccgaa ctcgagccgc gcatcccgaa aattcgcatc
     4081 ctcatccacc cggacggcat tgagcaactc cacattcgta ttgacgaaac ggcggtcccc
     4141 gccgccatcg tcggtatcga ccgcgtcgaa aacccaggaa aacacatcat ccaggtctcc
     4201 gccccgggat ttcaaaccgc ccaagtgcaa ttccagctac aagaaagcca aatcctgccc
     4261 gtcgaagtaa cgcttgaagc ggatacgccc agcaagcctg cccctgctcc cacaacccag
     4321 gttccccatt cgtataccgt ggcttctacc cccgaggcca atgcaccgtc ctcccacagc
     4381 accgaccttc ttatgtccat tcggctccaa tggactgctc ccaatggcga actcggtgag
     4441 acccacttca atggctcctg gatcgacccc ggagcggtgc gcgacagctt ccacgcggga
     4501 ggaggtctcg aattgcaggc ggacttgctt ttcggcaact gggccgtggg cttgctattc
     4561 ggcgtggatg accacggtca gccttccggc agtcacgagg ccgtgaaatc gctgtatccc
     4621 actatcgaat cggtacaaac caccaagtcg ttcagcgctt tcggcggaat cagcggacag
     4681 tggcatagct gccaacgctg tctcggcttc gtgggcgaaa cggccatcat catgcgtcaa
     4741 atggtccaaa ccttcgagat tcagccccaa tccggccccc ccgacccttg ctccctgtcg
     4801 tgggagcgcg acgttcccac cgtccgactg cttgccggac tccaaatccc cgtcggaagc
     4861 gacttctttg tcacacccta cctgtctttc gccggcggct ccacgtccgt cacccgactc
     4921 gaaccgtcgg acgatgctcc tcgttgcccg tcgaacaaga tcgagtcttc ggaaacgacc
     4981 ggtggctcga tcacattttt ttcggtcggt gtggcgggaa cctatgccct cgccctcgat
     5041 tgagcggggc gaaaccatcc cgaacgaaac gcaaagccta tcttagtaac tacattt
//
LOCUS       DAOSOR010000439         5090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_16554_length_5090_cov_4.603376, whole genome shotgun sequence.
ACCESSION   DAOSOR010000439 DAOSOR010000000
VERSION     DAOSOR010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16554_length_5090_cov_4.603376"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PK710_19100"
     CDS             <1..577
                     /locus_tag="PK710_19100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11894.1"
                     /translation="FSVGLLWFVGCTPSKVRWEPRQPSPRPMERRAQSSVEIFDGQAP
                     NRPNLEVGVLTAGQSRPKSDAAASQLKAKFIKRAAEIGCDAIVIRDASGAVSSKGGAL
                     EAGCLMWTEDAPAAVAGTPSGPNCTRDYDCQGEQVCNNGTCTEPAGATDPNGASERSG
                     CKTDLDCKGDRICEDGKCTAPEKEPKQDGGT"
     gene            complement(830..1015)
                     /locus_tag="PK710_19105"
     CDS             complement(830..1015)
                     /locus_tag="PK710_19105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11895.1"
                     /translation="MPRADEVSGTYQNACGLQESTLRDPGPDARACTFFSFKIFVFAP
                     IFSGPGATGNAPPQWKK"
     gene            1036..1695
                     /locus_tag="PK710_19110"
     CDS             1036..1695
                     /locus_tag="PK710_19110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HOT11896.1"
                     /translation="MVRRLDMVLSSIGKKELMALSGLGFIGFLVAHLAGNFTILSGAE
                     ALDGYAAKLEALGPLVWVAEAGMVTMALVHVLVGITLVVRNLQARGQRYAMKKSEGGR
                     TIGSRTMWLTGPWILVFVGIHLAQFTFPHKLTEEAPALSKLVAEQLSDPIWAGYYVVT
                     MLILALHVSHGFWSAFQTLGAAPSRSGYLRDTAVLLAFVFGAGFSLLAFMVFNVPDLL
                     K"
     gene            1715..3631
                     /locus_tag="PK710_19115"
     CDS             1715..3631
                     /locus_tag="PK710_19115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HOT11897.1"
                     /translation="MTQLDAKIPGGPLEQKWERHQFELKLVNPANKRKFEVIVVGTGL
                     AGASAAATLAELGYKVKCFGIHDSPRRAHSIAAQGGINAAKNYQNDGDSIYRLFYDTV
                     KGGDYRAREANVYRLATVSNLIIDHCVAQGIPFARDYGGTLANRSFGGAQVSRTFYAR
                     GQTGQQLLLGAYASLNRQIHLGGVTMFTRREMLDLIVVQGHARGILVRNLLTGEYERH
                     VADAVILATGGYANVFFLSTNAINSNVSAAWRCHKRGAFFANPCYTQIHPTCIPVHGD
                     KQSKLTLMSESLRNDGRVWTSKKKGDKRTPNDIDESERDYYLERRYPSFGNLVPRDVA
                     SRAAKERVDAGHGVGSTGVAVYLDFKDAIERVGREAIEKKYGNLFEMYERITGEDPYT
                     TPMMIYPALHYTMGGLWVDYDLMSTIPGLFVLGEANFSDHGANRLGASALMQGLADGY
                     FVIPYTLGGYLGSTKLPKVNDTDDAFKQAQDTAEKRVNKLMTAKGKRTPADFHKELGL
                     LLWEKCGMARNEQGLKEALSKIPKIREEFWENVIVPGDAKDLNQTLERANRVADYLEF
                     AELLVRDALERKESCGGHFREEHQTEEGEALRDDDNFCHVAAWEHIGDDQSPTRHIEP
                     LTFERVPLTQRSYK"
     gene            3632..4381
                     /locus_tag="PK710_19120"
     CDS             3632..4381
                     /locus_tag="PK710_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOT11898.1"
                     /translation="MELTLRVWRQAGPNAPGKFEDYQAKDVSPDMSFLEMLDFVNEDL
                     IRQGKEPITFEHDCREGICGCCGTMVNGKAHGGFEGTTLCQVHMRHFKNGDTIIVEPW
                     RSRVFPVLKDLMVDRSPFDKILHAGGYVSVNVGGAPDANAIPISPNAQDTAMDAAQCI
                     GCGACVAACPNGSAMLFVSAKVSHLDVLPQGKVETVRRTRAMIAKMDELGFGNCTNHA
                     ECEMACPKKISISHIARANRSFWRALFSHES"
     gene            complement(4465..4899)
                     /locus_tag="PK710_19125"
     CDS             complement(4465..4899)
                     /locus_tag="PK710_19125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11899.1"
                     /translation="MALGRSDRLAGGTGPTPIAPSGGASGRQASLALDRRGTRRRLRG
                     PPSRDFNVSAEDRGSLPVRRLKLGVEPWQQSPDIPNCEKAARKGDLLFPQAVIRSVTG
                     VCGYEVGDGQQTAIVRKMADVSAVQNVKAAVFATRALEAASW"
BASE COUNT         1136 a         1463 c         1452 g         1039 t
ORIGIN      
        1 cttttcggtg gggctgctat ggttcgtggg ttgcacccct tccaaggtac gctgggaacc
       61 gcgacaacca tccccccgcc cgatggagag gcgtgcgcaa tcgagtgtcg aaatatttga
      121 cggccaagct cccaatcgac ccaatcttga agtgggtgta ctgacagccg gccaatcgcg
      181 ccccaaaagc gacgcggcgg ccagtcaatt gaaggcaaaa ttcatcaaac gcgcggcaga
      241 aataggttgt gacgccattg tcatacggga tgcatcgggt gcggtttctt caaaaggagg
      301 tgccttggaa gccggctgct tgatgtggac agaagatgcg ccagccgcgg tggcagggac
      361 accatccggg ccgaattgca cgagagacta cgattgccaa ggagagcaag tttgtaacaa
      421 cggaacatgt acggaaccgg ctggcgctac ggatcccaac ggtgcttcgg aacgttccgg
      481 atgcaagacg gatttggatt gcaaagggga tcgcatttgt gaagacggaa aatgcacagc
      541 ccccgaaaaa gaacccaagc aagatggtgg tacctaacgc gctctagcgg tttgggcggt
      601 ggcacgtgga tgtccggcct tttccacgtt gggcacaaga agcataacgt ccccactgac
      661 ggcgagttct tgcctcaggt acggagcggc ggatgatcta ccttgccgtg atcggagccg
      721 agtcgcatgg ctcatgcaca aatgggtgag ccgtgcgacc ccacggcttg actataggtg
      781 atatctgtcg ccaggtcaga gagtccttcg cgaactaagg aaaacgtctc tattttttcc
      841 actgcggagg cgcattgcct gtcgcgcccg gcccggagaa aatgggtgcg aaaacaaata
      901 tcttgaacga aaaaaacgta catgcgcgag catctggccc cgggtcccgt agggtgctct
      961 cttgcaagcc acaagcgttc tggtaggttc cgctcacttc atcggcacgg ggcatatcgc
     1021 ctcgaggaga gcggaatggt tcgtcggctc gacatggtac tgtcgtcgat tgggaaaaag
     1081 gagttgatgg ccctatcggg attggggttc atcggattcc tggtcgcgca tttggcgggc
     1141 aacttcacga tcctatctgg ggcagaggcg ctggatgggt acgcggccaa actcgaggct
     1201 cttgggccgc tcgtttgggt tgcggaagcg ggaatggtta cgatggcctt ggtccacgta
     1261 ctggtgggca tcacgctggt ggtgcgcaat cttcaagcgc ggggacagcg ctatgcgatg
     1321 aaaaagtccg agggcggccg gacaatcggg tcacgaacga tgtggttgac cgggccgtgg
     1381 attctcgtgt tcgtggggat tcacctggcg cagttcacgt ttccccacaa gttgacagag
     1441 gaagcgcctg cgctttccaa actggttgcc gagcagttgt cggaccccat ttgggccggg
     1501 tattacgtcg tgacgatgct tattttggcg ttgcacgtga gccatggatt ttggagcgcg
     1561 tttcaaacgc tcggagcggc gccgtcacgc agtggatacc ttcgagacac ggccgttctg
     1621 ttggcattcg tatttggagc aggattttca ctgcttgcgt ttatggtttt caacgtcccc
     1681 gacttgttga agtaggccac aggagagatt cgacatgacg caactcgatg ccaaaatccc
     1741 cggtggtcct ctcgagcaga agtgggagcg ccaccaattc gaactcaagc tggtcaatcc
     1801 cgcgaacaag cgaaaattcg aggtgatcgt ggtgggcacg ggactcgccg gcgcctccgc
     1861 cgcggccacc ctcgcggaac tcggctacaa ggtcaaatgc ttcggaatcc acgatagccc
     1921 acgacgggct cattcgatcg ctgcccaagg tggcatcaac gccgccaaaa actaccaaaa
     1981 cgatggcgat agcatctatc ggctctttta tgacacggtc aaagggggcg attaccgcgc
     2041 cagggaggcc aatgtctatc ggctcgccac cgtcagcaac ctcattatcg accattgtgt
     2101 ggcgcaagga atccccttcg cgcgggatta tggcggaacc cttgccaatc gctccttcgg
     2161 aggcgcgcag gtttctcgta cgttctacgc ccgcggccag accggacagc agcttttgct
     2221 cggcgcctac gcctccctga accgacaaat ccaccttggc ggcgtaacaa tgttcacccg
     2281 ccgagaaatg ctcgatctta tcgtcgtgca gggccacgcc cgaggcatcc tggtgcgtaa
     2341 cctgttgacc ggagaatacg aacgacacgt tgccgacgcg gtcattctcg ccaccggcgg
     2401 ttatgcgaac gtgtttttcc tgtccaccaa cgcgatcaat tccaatgtga gcgccgcgtg
     2461 gcgctgtcac aaacgcggcg cctttttcgc caacccttgc tacacgcaaa ttcatccgac
     2521 ctgcattccg gtccacggcg acaagcagtc caaactcacc ttgatgagcg aaagtcttcg
     2581 caatgatgga agagtctgga cttcgaaaaa gaaaggcgac aagcgcactc ccaacgatat
     2641 cgacgaatcc gaaagggatt attacctcga gcgtcgttat ccgagtttcg gcaacctcgt
     2701 gcctcgcgac gttgcctctc gcgcggccaa agagcgagtc gacgctgggc atggtgttgg
     2761 gtcgacgggc gtggccgttt acctcgactt caaggatgcc atcgagaggg tgggccgcga
     2821 ggccatcgaa aaaaaatacg gaaacctctt cgagatgtac gaaagaatca cgggagagga
     2881 tccgtatacc acgcccatga tgatctatcc cgcccttcat tacacgatgg gaggattgtg
     2941 ggtcgactac gacttgatga gcaccatccc cggcctattc gttcttgggg aagcgaactt
     3001 ttccgaccac ggagccaatc gtctcggcgc cagcgctctg atgcaaggct tggcggacgg
     3061 ctacttcgtc attccctaca ccttgggtgg ctatcttggg agcacgaaac tgccgaaggt
     3121 caacgacacg gatgatgctt tcaagcaggc tcaagacact gcggagaagc gagtcaataa
     3181 actcatgacg gccaaaggca agcgtactcc ggcggatttc cacaaggaat tgggactgct
     3241 tctgtgggaa aaatgcggca tggcccgcaa cgaacaggga ttgaaagaag ccctttcgaa
     3301 aattccaaaa atccgcgagg aattttggga aaacgtgatc gttccgggag atgccaaaga
     3361 cctgaatcag acgctcgaac gcgccaaccg agtcgcggac tacctggaat tcgctgaact
     3421 tctcgttcgc gatgcgctcg aacgcaaaga atcgtgtggc ggtcacttcc gcgaagaaca
     3481 tcaaacggag gaaggggaag ccttacgcga cgacgataac ttctgccatg tcgccgcctg
     3541 ggagcatatc ggtgacgatc aatctccgac gcggcatatc gaacctctga cctttgagcg
     3601 cgttccactg acgcagagga gctataaata gatggaactc acgcttcgag tttggcgtca
     3661 ggctggtccc aacgcccctg ggaagttcga agactatcag gcaaaagatg tgtctccgga
     3721 catgtctttt ctcgagatgc tcgacttcgt caacgaagac ctgattcggc agggcaaaga
     3781 acccattacg ttcgagcatg attgccgcga aggcatttgc ggatgctgcg gcaccatggt
     3841 caacggcaaa gcccacggcg gttttgaggg caccacgctt tgccaggtac acatgcgcca
     3901 cttcaaaaac ggcgacacca tcatcgtgga accctggcgt tctcgtgttt ttcccgttct
     3961 caaagacctg atggtggacc gttccccctt cgacaaaatc ctccatgccg gcggatatgt
     4021 ctcggtcaac gtgggcggcg ctcccgacgc caacgccatc cccatcagtc ccaatgccca
     4081 ggatacggcc atggacgcgg cgcaatgcat cggttgcgga gcctgtgtcg ccgcctgccc
     4141 caatggttcc gccatgctat tcgtcagcgc caaggtctct catctcgacg tccttcccca
     4201 aggcaaagtc gaaaccgttc gtcgcacgcg agccatgatt gccaagatgg acgaactcgg
     4261 ttttggcaac tgcaccaacc atgcggaatg cgaaatggct tgcccgaaga aaatttccat
     4321 cagccatatc gcgagagcca accgcagttt ttggcgcgcc ttgttctccc acgaaagctg
     4381 aaccttcgtg ggcgtgccga ccgatgctca ccgcgatctt gcccgcgaat ctcggttcct
     4441 cccaccataa gcgataccaa gcccctacca cgacgccgct tcaagagcgc gtgttgcgaa
     4501 caccgcggcc ttcacgttct gcactgcaga aacgtccgcc atctttcgaa cgatagcggt
     4561 ctgttgtcca tcaccgactt catagccgca gaccccagtc acagatcgga tgaccgcctg
     4621 gggaaaaagt aggtctccct ttctcgctgc tttctcacaa ttcgggatat caggggattg
     4681 ctgccagggc tctaccccca gcttcaggcg tcttaccggt agggagcccc ggtcctcagc
     4741 agaaacgttg aagtcgcgcg aaggaggtcc tcgaaggcgc cgtcgagttc ctctccggtc
     4801 aagagccaac gaagcttgtc gtccactcgc tcctcccgat ggtgcgatgg gagttgggcc
     4861 agttcctccg gcgagccggt cagatcgacc aagagccacc gctgcgggag aaagcggatt
     4921 tccgaggcta ccccttcagg ccaacggatc tcgcataagt ctgcttcaag ggtcacacca
     4981 tgctttgatg atgaggcttt gacgcgactg acggctagag gggcagttcc ctcgtcgagc
     5041 ttcagatcca ccccccaacg gacccctcaa tccaaaggca aggggaccgt
//
LOCUS       DAOSOR010000440         5085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_16589_length_5085_cov_4.151889, whole genome shotgun sequence.
ACCESSION   DAOSOR010000440 DAOSOR010000000
VERSION     DAOSOR010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16589_length_5085_cov_4.151889"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PK710_19130"
     CDS             complement(<1..314)
                     /locus_tag="PK710_19130"
                     /inference="COORDINATES: protein motif:HMM:NF014077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOT11900.1"
                     /translation="MMDDDARPLLLVTNDDGYGAPGLEAMVAALEAWATVLVIAPSHE
                     KSACSHQLTLGRPLQRKKLGPHRYWVDGTPADCVYLGLLGGERVSPRIPDLVVSGLNA
                     GL"
     gene            complement(351..836)
                     /locus_tag="PK710_19135"
     CDS             complement(351..836)
                     /locus_tag="PK710_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOT11901.1"
                     /translation="MKWAPLALLPRSALTILREMGRHILRHPVVGVLAIARDNQGKYL
                     LIRRADTGTWTMPGGTLEWGETLRDALFREVQEESGAKVLRVGRLVGVYSRPDRDMRF
                     HAVTIAVEVTVDASHLEPVNRLEVREARLFDATEIPTPLAMGTDAMLQDAMAGGEPTF
                     E"
     gene            complement(918..1877)
                     /locus_tag="PK710_19140"
     CDS             complement(918..1877)
                     /locus_tag="PK710_19140"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOT11902.1"
                     /translation="MFPTKLQQLLGIELPIIGGTMMDLSTAPFVSAISKAGALGIIAS
                     AIYRDENTFRNDLKAVRDATDRPFGVNINLFPMMKKLNNFKYLDIMGEEGVKIVETSG
                     FSPPEDLVARIKSAGMIWMHKCVGVRYAKKAESMGADAVTVVGYENGGATGNLNIGTL
                     VLVPSTVDAVSIPVIGGGGVVDGRTLLAVLSLGASGAIVGTRLLLTEESPIHPRLKQA
                     LVDATELDTDVVMRSVGFAHRVWMNEPAKKAQEIEAKGGGIEALYPYVSGAAAKRMFE
                     TGDLQAGTISCSQAVGLIKEIKPIRETLLEMVDQAKQIQARLG"
     gene            complement(1982..4246)
                     /locus_tag="PK710_19145"
     CDS             complement(1982..4246)
                     /locus_tag="PK710_19145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11903.1"
                     /translation="MSKYAVDDDDIWTEKVWRVRSADGDSKEEVGPVSLDQVRRGIEA
                     GKLDAAAKVARHGSDNWLSAKKLIDAAITRDKALVRKSISTAELSLVEEVSRVQLNDP
                     DLTTPGQRMPVSKSAKADRSVAQGALVLLALLAVTGACWLSFRFIGPRPSDAAINPRA
                     EHFPRSTVAVIESAIRDDMIEMTEVPNAYVSAWLARHVCGGIDIEKRLRSAKGESLSK
                     MHDLGVVMLSAQREWWDAMRCGDNLRSHLLSPRQTTIVFEHGKDSAVVVGLRMDQHIM
                     PEVQGRRAHTFSGLTGFCQKQDKDENACQPLGLAGLTYGQTSFFGSFGDVEAFAAAIT
                     SAHHESSSNQDTLAELAEHASNAPSRTRLVRPSTIPWDGLCEIVAPRKDKESFMNACF
                     PRAAKTRLDDAVLKTRGILIERESLTTAQRVHWSSVLLARSREAGRWIEDELLDLRRD
                     WLSLLVDNESMLIRSLTDSRTTTQQRLWAAASDAWLRALRHSRVSKTGAAVRWELSAK
                     LDPEEQKLVREAMQNEDPEEKAMRAVLEAMALGTPIPIDALAVFLGDEIATWSAAPRA
                     TEGDCRRLLTAVESVSVDPNALNLVGLRDSLIRTFDPKQCVGTPLPESYRACLLQASS
                     LQALEACKPPRSPFVKHAQRRLQGQWTVSNVDGSKSSQLSYLLRASLQRCRLELDNQK
                     IAFDCLEDGGVDVLGVEAQDTDKAVLLLPLEKSRVPKRVVFGESEDSFTMPDFAPGVE
                     LTFRRSVFHSLLKP"
     gene            complement(4335..>5085)
                     /locus_tag="PK710_19150"
     CDS             complement(4335..>5085)
                     /locus_tag="PK710_19150"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOT11904.1"
                     /translation="AHHASFSLSDANVQPSTLLRSGLIVLFSLQGFEIIAVPASSVRS
                     RPFMATATILSLSFAGVLYFGLHLACVQAIPELHASKAPLVEAARALGGPCLAGTVMV
                     GTSISALGIALGMIAMSPRYLSALGRSDGLGTWFGVTNERGVPRRALILTSCLVLLFV
                     QVGRLEQLFAMSSIAVLAQYGSTSASLLWMGAKGQWGLGRKDLVVGVVALAASLVVLT
                     GATWQETFRAVALLALGWLIKFAVSMGRRKP"
BASE COUNT         1112 a         1488 c         1417 g         1068 t
ORIGIN      
        1 ttcagccccg cgttcaagcc cgaaaccacg agatccggaa tacgaggtga cacgcgctcg
       61 ccccccaaca gcccaaggta cacacagtcc gctggcgtcc catcgaccca atagcgatgc
      121 ggccccagct tttttctctg caggggcctt cccaacgtaa gttggtgaga acaggcgctt
      181 ttttcatgag acggcgcgat caccaacaca gtcgcccacg cctccagcgc cgcaaccatg
      241 gcctctagac cgggtgctcc atacccatca tcgttggtca cgagcaaaag agggcgcgca
      301 tcgtcatcca tcatgaaaac atcattccca tggctcatac cgcaggcgtc tcattcgaac
      361 gtgggctcac cgccagccat ggcatcctgc agcatggcgt cggtccccat cgccagcgga
      421 gtggggattt ctgttgcatc gaatagccgc gcttcccgaa cttcaagacg attgaccggt
      481 tcgagatggg aggcgtcgac agtcacctcg accgcaatgg tcacggcatg aaaacgcata
      541 tcccggtcag gacggctata gaccccgacc aatcttccga cgcgcagcac cttggcacct
      601 gattcctctt gcacctctcg aaacagcgcg tcgcgcaagg tttcccccca ctccagggta
      661 cctccaggca tggtccaggt accggtatcg gcgcgacgaa tcagcaaata tttcccttgg
      721 ttatcgcgcg cgatggcgag cacgccaacg acgggatgcc gaaggatatg tcgtcccatt
      781 tctcgtagaa tcgttagcgc ggatcgaggc aatagcgcga ggggcgccca cttcatgaat
      841 gcattcctat gatcaattcc cgtggggaag cagcttgaaa atgatttcgt agcacgtccg
      901 ccgtcgttcg tcctgcctca cccaagacga gcctgaattt gcttggcctg gtccaccatc
      961 tcgagcagcg tctcacgaat cggtttgatt tctttgatca gaccgaccgc ctgggaacag
     1021 gagatggttc ccgcttgcag gtctccggtt tcgaacattc gctttgccgc ggctcccgat
     1081 acatagggat atagcgcttc gatccctccg cccttggctt caatctcctg cgcttttttc
     1141 gcgggttcgt tcatccatac ccgatgggca aatcccaccg aacgcatcac cacatccgta
     1201 tccaactccg tcgcatcgac aagggcttgc ttcaatcgcg gatggatcgg gctctcttcg
     1261 gtgagaagca agcgcgttcc cacgatcgcc cccgaggcac cgagcgacag gaccgccagc
     1321 aacgttcgac catccaccac tccccctcca ccaatgaccg gaatgctcac ggcatcgacc
     1381 gtggaaggaa cgagcacgag agtgccaatg ttgaggtttc ccgtagcgcc accattttcg
     1441 tagcccacca cggtcacggc atccgctccc atgctttcgg cttttttggc gtagcgaacg
     1501 cctacgcatt tgtgcatcca aatcattccg gccgacttga tgcgcgccac caaatcctcg
     1561 ggcggcgaga aaccggaagt ctccacgatc ttgaccccct cctcccccat aatgtctaaa
     1621 tatttgaaat tatttagttt tttcatcatg ggaaagagat tgatgttgac accgaacggc
     1681 cggtcggtgg catcgcgaac cgctttgaga tcatttcgaa acgtgttttc atcgcggtag
     1741 atggcggaag cgatgatgcc gagagcccct gccttcgaga tcgcggaaac gaagggagcg
     1801 gtagataggt ccatcatcgt accgccgatg atgggcagtt cgatccccag aagctgctgg
     1861 agtttcgttg gaaacatggc tgactcctat gctcgtggag cggttgaaaa aagcgtctgc
     1921 aagttccccg gcaccgtacg gaacgccgcc gctcgaagca atgggcaatg agcgcttcgc
     1981 gtcagggctt tagcagtgaa tgaaatacgc tgcgacgaaa ggtaagttcg actcctgggg
     2041 cgaagtcggg catggtgaag gagtcttcgg attcgccgaa gaccacccgt tttgggacac
     2101 gcgatttctc caaaggtagc aacaataccg ccttatcggt gtcctgggct tccaccccca
     2161 acacgtcgac cccgccatct tcgaggcaat cgaaggcaat tttttgattg tcgagttcca
     2221 agcggcaccg ctgtagcgat gctctcaaga gataggatag ttggctgctt ttacttccgt
     2281 cgacgttcga gacggtccat tgtccctgga ggcgtcgttg tgcgtgcttt acgaagggcg
     2341 agcggggagg tttgcaggct tcgagggctt ggagggaaga ggcttggagc aggcaggctc
     2401 gatagctttc gggcagcggc gtgcctacac attgtttggg gtcgaaggtg cggatcaacg
     2461 agtctcgaag tccgaccaga ttgagggcgt tcggatcgac ggaaacggat tccacggcgg
     2521 tcaacagcct tctacaatcc ccttcggtgg cgcggggagc agcggaccag gttgcgattt
     2581 cgtcacctag gaagacggcg agcgcgtcaa tgggaattgg ggttcccagg gccatggcct
     2641 cgagaacagc ccgcatggct ttctcctccg gatcctcgtt ttgcatggcc tcgcgaacga
     2701 gtttttgctc ttcaggatcg agtttcgcac tcagttccca acgcacggca gcgccagttt
     2761 tcgacacacg agaatgcctc aaggctcgca accacgcatc cgacgccgcg gcccacaacc
     2821 gctgttgcgt cgtggttcga gaatccgtca aagagcgaat cagcatcgat tcattgtcca
     2881 cgagcaagga aagccagtct cttcgcaaat cgagcagttc gtcttcgatc catcgtccgg
     2941 cctcacggct tcgggcgagt agaacggaag accaatgcac gcgttgtgcg gtggtgagcg
     3001 attcgcgttc gatgaggatt ccgcgtgttt tcaataccgc atcgtccaga cgcgttttcg
     3061 cggctcgggg aaagcacgca ttcatgaagg attctttgtc ttttcgaggg gcaacgattt
     3121 cgcaaagtcc atcccagggg atcgtcgatg gtcgaaccaa acgtgttcgg ctgggggcgt
     3181 tgcttgcatg ctcggcgagt tccgcgagcg tgtcttgatt cgagcttgat tcgtgatggg
     3241 cagaagtgat ggcggcagcg aacgcttcta catccccaaa agagccaaaa aacgaggttt
     3301 gtccataggt tagcccggcg aggcccaacg gctggcaggc gttttcatcc ttgtcttgtt
     3361 tttggcagaa gcctgtcaag ccgctgaagg tatgtgccct tcgaccttgg acctcgggca
     3421 tgatgtgttg atccatcctc agccccacga cgaccgcaga atccttgccg tgttcgaaaa
     3481 cgatggtggt ttgtcgtggg ctcagcaggt gggagcgaag gttatcgccg cagcgcatgg
     3541 catcccacca ttcgcgttgt gccgagagca tgaccacgcc aagatcatgc attttggata
     3601 gggattcgcc ctttgcggag cgcaaacgct tttcgatatc gatacctcca cacacatggc
     3661 gagccaacca cgcactgaca tatgcgttgg gcacttcggt catttcgatc atgtcatcgc
     3721 ggatggcaga ctcgatcacg gccacggtag agcgtgggaa atgctcggca cgagggttta
     3781 tcgctgcgtc agaagggcgt ggaccgatga aacgaaaact caaccaacat gcgccagtca
     3841 cggcaagcaa tgccaacaag acaagggctc cttgcgccac gcttcgatcg gctttggcgc
     3901 tcttggagac gggcatcctt tgtccgggag tggtgaggtc aggatcgttg agttggacgc
     3961 gagacacctc ttccaccaaa gataattcgg ccgtggaaat ggatttgcga accagggctt
     4021 tgtcccgcgt gatggccgca tcaatcagtt ttttggccga aagccagtta tcggaaccgt
     4081 gtcgagcgac tttggctgcc gcatcgagtt tgccagcctc gatccccctt cgtacttgat
     4141 ccaaactgac gggcccaacc tcctctttgc tgtctccatc cgcagaacga acacgccata
     4201 ctttttctgt ccagatatcg tcgtcatcca ccgcgtattt cgacatgaca atcagtgact
     4261 ttcatgacga gtagcacagc atgtcaaggg ataactcggt acagacacaa gaccgttcgc
     4321 gcggcattcg cggttcaggg ttttcgtcgc cccatgctca cggcaaactt gatgagccag
     4381 cccaaagcca acaaggcgac ggctcggaag gtttcttgcc aggtggctcc tgtcagcaca
     4441 acgagggaag ctgccaaggc caccacaccc acgacgagat ctttccttcc caggccccac
     4501 tgaccttttg cgcccatcca cagcaaggaa gcggatgtgc ttccgtattg ggccagcacc
     4561 gcaatgctcg acatcgcaaa aagctgttcc aagcgcccta cttgcacgaa aagcagcacc
     4621 aggcacgaag tgaggatcaa cgctcgtcga ggcacccctc gttcgttggt cacgccgaac
     4681 caggtcccca agccatcgct acgcccgagg gcggacaagt atcgcggtga catggcgatc
     4741 atgccgaggg caatccccaa ggcggagatg ctggtaccta ccatgacggt cccggcgaga
     4801 cagggtcctc cgagcgctct tgctgcctcg accaagggag ctttgctggc atgaagttcc
     4861 ggaatggctt ggacacaagc gagatgaagg ccgaagtaca acacgccagc gaaggagaga
     4921 gagagtatcg tggccgtagc catgaagggg cgagagcgca ccgaactagc tggaactgca
     4981 ataatttcaa agccttgcag cgagaacagg acgatcaggc cgctgcgcaa cagtgtggaa
     5041 ggctgcacat tcgcgtcgga taaagaaaac gatgcatgat gggcg
//
LOCUS       DAOSOR010000441         5085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_16590_length_5085_cov_4.037972, whole genome shotgun sequence.
ACCESSION   DAOSOR010000441 DAOSOR010000000
VERSION     DAOSOR010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16590_length_5085_cov_4.037972"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /gene="ftcD"
                     /locus_tag="PK710_19155"
     CDS             complement(<1..1188)
                     /gene="ftcD"
                     /locus_tag="PK710_19155"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOT11905.1"
                     /translation="MAIQLVECVPNFSEGRDQSIIQAIADAMRSVEGVSVLDVDPGAD
                     TNRTVYTFVGAPEAVLEAAFRAIRVGVERIDMQKHHGEHARLGAADVTPFVPVSGVTM
                     EECVKLAHRLGKRVGEELGVPVYLYEAAATSEQRRSLPDIRSGEYEGLAKKLEDPAWK
                     PDYGPAEFKPRSGAFVIGARKFLIAYNINLNTRDPKLANEIAIEVRERGRLQRDANGN
                     KILGEDGVPLRTRGRLAECKATGWYIEDYGQAQVSMNLTDHHVTPVHLALETVREEAQ
                     KRGLVVTGSELVGLMPKDAILEAGKYYLKRQGRSWGVPERELIDIAIASMGLSQLSPF
                     DPNKKIIELSVEKSDGKLVQRSVTEFVDECSSESPAPGGGSVAALSGALGAGLVSMVA
                     NLTV"
     gene            complement(1279..2415)
                     /locus_tag="PK710_19160"
     CDS             complement(1279..2415)
                     /locus_tag="PK710_19160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT11906.1"
                     /translation="MSHLDAPLPVDRPDLLSIRAALRRLLVRFGPEYWRTCDSQRAFP
                     SEFMRTMAEGGYLGTLIPEAHGGLGLGPSVASVIVEEINRAGGDGAVVNAQMAICGTL
                     VREGTEEQKAYLPQIAAGTTRLLTVAATEPDSGADMSHLESVARRQGQDWVVDAKKVF
                     ISLAEHTDLMILLVRTEEGPTLFLLDLREFGTEIEIRPVDMIANRMTTMLFIDGLCVP
                     DRCRLGSVGGGLRGLMKGFAPRRVLAAAESIGNARFFLDKSVQHAIDRRTFHRPIGQN
                     QGVQYPLAQAYAKVEAADLMRWDAIRYLEDGRDGGGRSALAKILASEAAWETARAALT
                     TFGGWGLASEVHVERKLRDATIFVFNNMLLSYVAEKVLGMPKSF"
     gene            complement(2412..3392)
                     /locus_tag="PK710_19165"
     CDS             complement(2412..3392)
                     /locus_tag="PK710_19165"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOT11907.1"
                     /translation="MSSDDVKWDVLVESTLLTLLQTAGEGILVFDAAGACKMVGKKIG
                     EFFATDVSKLIGKVRTEVMLVLSKACEEPEAFLALVGGNDNDDPPHVLGEIEVRSPRR
                     VLVLRSTPIKLEGQYLGRLLWMRDVTREASAERARRHLLQRIELLTPVDALTGLANRR
                     RFTEEHEREHGRAMRAWDSYAVLRLDIDDMGGINEHFGVPIGDLVIERVAELLRAGRR
                     DYDVVARLANDEFAILLPGADAVAARAVADRVVMTVADAVKENQQGPQITLSIGVAVC
                     VPPTGESAADIMGRCGMALQFAREAGKGQVHLDLISGDSKGETGEERSTS"
     gene            complement(3575..4837)
                     /locus_tag="PK710_19170"
     CDS             complement(3575..4837)
                     /locus_tag="PK710_19170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11908.1"
                     /translation="MRTNRWRQLSMPILVAGFAAPTVIGCGSIPKPPGLPDVPGGCPD
                     VASVEAIAKIDFAQEFGFDVQGAAKVKSALTASVELQGLQAEIEGELKAACGKLASDL
                     GATPDGNDAVAACKAAGKAIGDLKAKAGGTFKLAVVPPKCAASMNAMADCAASCDASV
                     QPGSAKVSCEGGELSGSCGAECSGSCEMSAGGSCGGTCEGSCEAGFSGTCDGTCDGKC
                     DGKNTKGQCSGKCEGKCEGGAKGNCAGSCKGNCELKAGAKCEGTCTGSCSVDFEAPKC
                     TGEVKPPSMSAECSAECDAKVSGKLECTPASVTFVAKGAVDAEAAAKLKAAIEANLPA
                     VLKVAIGMKDKAVKVAGSVKGVVEGLEGAVKGMVSGGPTMAAKVGACISAPFKGAFDA
                     AASIQANVDVSVSVQASASAEGSASAGS"
BASE COUNT         1138 a         1778 c         1120 g         1049 t
ORIGIN      
        1 aaccgtcagg ttagccacca tgctcaccaa accagctcca agcgcaccag acagcgctgc
       61 cacagagccc cctccaggag ccggtgactc cgaagaacac tcatccacaa attccgtcac
      121 gctgcgctgc accagcttgc catcgctctt ctccacacta agctcgatga tctttttgtt
      181 cggatcgaaa ggcgaaagct gagacaaccc catcgaggca atcgcgatat cgatgagttc
      241 acgttcgggc actccccatg atcgtccctg cctcttcaag taatacttgc ccgcttcgag
      301 gatggcgtcc ttgggcatca gtcccaccaa ttcgctcccc gtaacgacaa gcccacgttt
      361 ttgtgcttct tcgcgaacgg tttcgagagc caaatgcacg ggcgtgacat gatgatccgt
      421 aaggttcatc gacacttgcg cctgtccata atcttcgatg taccaacccg tcgccttgca
      481 ttccgcgagt cgcccacgcg ttcgaagcgg caccccatcc tccccaagga ttttatttcc
      541 attggcatcg cgttgcaggc gtccgcgctc ccgaacctca atcgcaattt cattggcgag
      601 tttcggatcc cgcgtattca agttgatgtt gtaagcgatc aaaaactttc gggcacctat
      661 gacgaaagca ccagatcgtg gcttgaattc cgcgggaccg taatccggct tccacgccgg
      721 atcttcgagt tttttcgcaa gcccctcgta ctcacccgaa cgaatgtccg gaaggctgcg
      781 acgctgctca ctcgttgcag ccgcttcata gaggtatacg ggtacgccca attcctcacc
      841 cacacgcttt cccagtcgat gagcaagctt cacacactct tccatcgtca cgcccgatac
      901 gggaacaaag ggcgtgacat ccgcggcacc aagacgcgca tgttcaccgt gatgcttttg
      961 catgtcgata cgctcgacac ccacgcgaat cgctcggaat gccgcttcca acacagcctc
     1021 cggggctccc acgaacgtgt acaccgtgcg attcgtatcc gccccaggat cgacgtccag
     1081 caccgatact ccctctacag agcgcatcgc atcagcaatc gcttgaatga tcgattgatc
     1141 acgaccttca gagaagttgg gaacgcattc gacgagttgg attgccatga tcacctcatg
     1201 aacgaagatt tctgcccatg ccccatcgcc cctcgttttg caagacaacg taatgcataa
     1261 cgagccacgc cgtgccgttt agaatgactt gggcatccca agcaccttct cggccacata
     1321 actcaacagc atgttgttga atacgaagat ggttgcgtct cgaagcttgc gttcgacatg
     1381 aacctccgag gcaagtcccc atcctccaaa ggttgtcaaa gccgccctcg ccgtctccca
     1441 cgcagcttcc gaagcaagaa ttttcgcaag cgccgaacgc ccccccccat cacgcccgtc
     1501 ttcgagatac cgaatcgcat cccaacgcat caaatccgcc gcctccacct tggcatacgc
     1561 ttgcgccagc ggatactgaa ccccttgatt ctgaccaatc ggacggtgga aggtcctgcg
     1621 atcgatggcg tgttgcacgc tcttatccaa aaaaaaccgc gcatttccaa tcgattccgc
     1681 cgctgccaac acgcgacggg gcgcaaagcc tttcatcaat ccgcgcaacc ctcctcccac
     1741 cgagcccaaa cgacaacgat ccggcacaca aagcccgtcg atgaacaaca tcgtcgtcat
     1801 acgattggct atcatatcca ccggtcgtat ctctatctcc gtgccaaact cccgtaagtc
     1861 caacaaaaac agggttggcc cctcctcggt gcgaaccaac agaatcatca aatccgtatg
     1921 ttccgccaag gaaatgaata ccttcttggc atcgacaacc caatcttgtc cttgccgacg
     1981 agccacgctc tcgagatggg acatatccgc accactatcg ggctccgtcg cagctaccgt
     2041 gagcaaccgc gtggttcccg cagcaatctg cggcaaatac gccttttgct cctccgttcc
     2101 ctcacgaacg agcgtgccgc aaatggccat ctgcgcgttg acgaccgcac catcaccgcc
     2161 cgcacgattg atctcctcaa cgatcacact tgccaccgaa gggcccaatc ccaatccacc
     2221 atgtgcttca ggaatgaggg tccccaagta accgccctct gccatggtgc gcatgaattc
     2281 tgaaggaaaa gctcgttggc tgtcacaagt gcgccaatat tctggcccga aacggacaag
     2341 gagccttcgt aacgccgcgc gaatcgagag cagatccggc cggtccacag gaagcggagc
     2401 atcgagatgg ctcatgacgt cgaccgctct tctcccgttt cccctttcga atctcccgag
     2461 atcaggtcca aatgaacttg tccttttccg gcttcccgcg caaattgcag cgccataccg
     2521 caacgcccca tgatgtccgc cgcactctca cccgtgggcg gcacacaaac cgcaacgccg
     2581 atactgagcg taatctgagg cccttgttga ttttccttga ccgcatccgc cacggtcatc
     2641 accacccggt cggccaccgc gcgcgccgcc accgcgtcag cccctggcag caatatcgca
     2701 aattcgtcgt tggccaaccg agcaaccaca tcataatccc gacgccccgc tcgtaacaat
     2761 tccgccacac gctcgatcac caagtcccca atcggtacac caaagtgctc attgatcccg
     2821 cccatatcgt caatgtccaa ccgcaacacc gcatacgaat cccatgcgcg catcgcccgc
     2881 ccatgctctc gctcatgctc ttccgtaaaa cgccgccgat tcgccaaacc cgtgagcgca
     2941 tccaccggcg tcaacaattc gatccgctgc agcaaatgac gtcgcgctcg ctccgcactg
     3001 gcctcacgag tcacgtcccg catccacaat aaccgcccca aatactgccc ctcgagcttg
     3061 atgggcgtac tacgcaacac caacacgcga cgaggcgagc gcacttcgat ttctcccaaa
     3121 acgtgaggag gatcgtcgtt gtcgttgccg cccacaagag ccagaaacgc ctcaggctcc
     3181 tcacaagcct tgctgagcac gagcatcact tccgtgcgca ctttcccaat cagcttgctg
     3241 acatcggtgg caaaaaactc ccctattttc ttgcccacca ttttgcaggc cccagccgca
     3301 tcgaaaacca aaattccttc ccccgccgtt tgaagcaagg tcagaagcgt cgattcgacc
     3361 agcacatccc acttgacgtc atcgctcgac atgaccgcct taccatgcca cgaatgacca
     3421 gcttaggtcg agattcggga gcagtaagcc gatatcctcg ttcgctcccg cgttcgaaag
     3481 gatagatgaa ccctgcgagt gagcatctcc cttgctcccc ccaaagcaag agaatgccca
     3541 aacgatacac ctgaaaggat gggtcggcag aacgtcagct tcccgcggaa gcacttccct
     3601 cggcagaagc actggcttgc acgctcacgg atacatcgac attggcctga atgctcgccg
     3661 cagcatcaaa cgctcccttg aacggtgccg agatgcaagc accaaccttc gccgccatgg
     3721 tcggaccgcc agaaaccatg cccttgaccg ccccttcgag cccctcgaca acacccttca
     3781 ccgaaccggc gaccttcacc gccttgtcct tcatcccaat cgcgactttg agaaccgcgg
     3841 gaagattagc ctcaatcgcg gccttcagct tggcggcagc ttccgcatcg accgcacctt
     3901 tggcaacgaa cgtcacagag gcaggcgtgc attcgagctt gcccgatacc ttggcgtcac
     3961 attccgcact gcactcggca gacatcgatg gcggtttgac ttcccccgta cacttgggag
     4021 cttcgaagtc cactgagcac gatcccgtgc aggtcccttc gcatttggct cctgccttga
     4081 gttcgcaatt tcccttgcaa ctccccgcac aattcccttt tgcccctccc tcacacttgc
     4141 cttcgcattt gccagaacac tggcctttcg tattcttgcc atcgcacttg ccatcgcaag
     4201 tgccatcaca tgtaccactg aaaccggctt cacaactgcc ttcgcaagtg ccgccacagg
     4261 aaccaccggc gctcatctca caagagccgc tgcattccgc gccacaagat cccgacagct
     4321 cgccgccttc gcagctaacc ttggcactcc caggctgcac actcgcatcg caggaagccg
     4381 cgcaatcggc catcgcgttc atcgatgccg cacacttggg cggtaccacc gcaagcttga
     4441 acgtgccgcc cgctttcgct ttgagatctc caatggcttt ccccgcggct ttgcatgccg
     4501 ccaccgcatc attgccatcc ggtgtggctc ccaaatcgct cgcgagcttg ccacaagccg
     4561 ccttgagttc tccttcgatc tccgcctgca acccttgcag ttctaccgaa gccgtcagag
     4621 cacttttgac tttcgccgct ccctgcacat caaagccaaa ttcttgcgca aaatcgattt
     4681 tggcaatcgc ttccacactc gccacatccg ggcaacctcc cggaacgtcc ggtagtcccg
     4741 gcggtttcgg aatcgatcca catccgatta ccgtgggagc cgcaaatcca gcgaccaata
     4801 tcggcatact cagttgacgc caacgatttg tcctcatcga attacctcca gagcgggatg
     4861 ctgtcgctac caattcgaca tacaatgcaa gggcattggg attacggaaa ccttccaatc
     4921 atcaccgttg ccatagcctt tcgcctattc tcttcacccg ccgcgcggaa catatcgcac
     4981 cttcacgggc tgcttgtcta gcaagaagaa ttcaaaattt ttccccggca cgcgttcacg
     5041 gcagaaaata tcttggagtt ccgctcactt ccatcgcttc cccac
//
LOCUS       DAOSOR010000442         5084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_16594_length_5084_cov_4.077550, whole genome shotgun sequence.
ACCESSION   DAOSOR010000442 DAOSOR010000000
VERSION     DAOSOR010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16594_length_5084_cov_4.077550"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1653
                     /locus_tag="PK710_19175"
     CDS             <1..1653
                     /locus_tag="PK710_19175"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HOT11909.1"
                     /translation="SWHYRSQDEGLIAFSNQHYYEGRLCSFPTPPSPEEELGVSLRRV
                     QGVFYRTGPSKLLRTNPVEAQAIVDEVKRRFNNSPDSCPSLGIITFNIQQRAHIEALL
                     RDTDDERIIEALESRHEGLFVKNLENVQGDERDVILFSTAFSVNDKGVLPLQFGPLNR
                     QGGERRLNVAVTRARRQVIVFSSFDPSQLRAEETSSVGIQLLRAYLDAAQRGADAMRV
                     ASRRPLVDHHRDEMARRLGQAGLAVRKDVGISSFRVDIELALQERPEQPCVALLLDGE
                     SWYARSTVSDRDGMPALVLGKMMRWPVVERVWMPEWLADPEAVIARLAKAVHAPPARA
                     VEKKRSQTLVMSTDFSASKPEQPAIQTTVEPSLLGQTPFQEAIGQKVGNKEQLDALQE
                     SYGVQKMVVELANRMLEVEGPVHEERLARFLMVSFGIQQLHKKRKDEVLGVVEKHIRK
                     ERDFFWPAGMDEATWVDYRPDPEYKRALHQIELVELGNAMADLGKKLGGGFKEELFRQ
                     TLGVFGIQRLTAQARERLEASLALMVERSRLVIRGDEVIEVL"
     gene            2053..3933
                     /locus_tag="PK710_19180"
     CDS             2053..3933
                     /locus_tag="PK710_19180"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOT11910.1"
                     /translation="MKTSRAYRLGWSLVFGWALVAACARPQVTGPQASEPPSAVDDQK
                     PGDGQAFEPPAPNLITTNSEASPSREPVAEFIRLVLPRDDAFLSTPFSPANPARYAGW
                     RESGRLHVGKGNLHYAEAIDTQRLVLAMSTTEGTVQVYDRATRSLKHVVSVANLDDFG
                     RSAMAPWPEGSALHGFLAGRKDGLWLYDATSTRAPQRLDDAFLWTMRWSPDQTVLLGA
                     SSDTKSQTSVLYFYRRTSPDKLERVGAYNLPERVEGLDLSRDNRHLLLSLYPSNTMVL
                     ADLREGRILWRVPAPEFASDVSFSPDGQAVAAGGAGVVLLDATRPTRRTKYTGFGNNV
                     ARVRFSPSGDALAVSSYDGKVRIFSWDLDSLSLTLTKTLSHTGTANVYALHFLDDGDT
                     LMSSSGDQTVRFWSGPAGRQPLKNPWQQGQWKPLVVVEPMAGAGGLERPFDWSALDRE
                     ARAKLFGELRAEPRHRPARLDGPPSPSAIVPGNYACRVGSLYKLRDCIVQKDSAGHVL
                     LEVLEGNIIPMRGVLFDDGPVVRFEGASTAVRPFACMACQDRCYLFDDSCSCRPEPPD
                     RIRQCLSQPLHAVFRKVGNRWQGVLAYALSQDDILNPVPLPADAPFSDSRGRFTIELI
                     AR"
     gene            complement(4119..>5084)
                     /locus_tag="PK710_19185"
     CDS             complement(4119..>5084)
                     /locus_tag="PK710_19185"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOT11911.1"
                     /translation="LEPEFPFITMLMHADTPYQLMKGKNIGNETAVHLSLPRLRQGKI
                     DAFSAVLFVESASQNENTALYCQRATKKIDALLEDHSGLLRLALTPEDFKTNRYSGHI
                     SVFRTLEGSFCLRHGIYRIEDLYASGIRMIGLTWENDHDFATSWVYHGPIGLTERGKN
                     AVVRMNHLGIIVDVSHMSDRAIDDVLAISQAPIVASHSNARSVCPHPRNLNDAHIRAI
                     AEKGGLIGVNFYPPHLVQSGKATLAHVVQHIRYLRDVGGIGCVGLGSDFDGISKGPSG
                     LETAAELPRLRETLLAEGFSEPEVAAIFGGNFLRLFNKVQALGKH"
BASE COUNT         1056 a         1267 c         1582 g         1179 t
ORIGIN      
        1 tcgtggcatt atcgttcgca ggatgaaggg ctgattgcct tttcgaatca gcattattac
       61 gaagggaggc tttgttcctt tcccacgcct ccgtcgccgg aggaggaatt gggggtgtcg
      121 ttgcggcgtg ttcaaggtgt attttaccgc acggggccga gtaagttgct tcgaaccaac
      181 ccggtcgaag cgcaggcgat cgtcgacgag gtgaagcgtc gtttcaataa ttcgccggat
      241 tcctgtccct cccttgggat tatcaccttc aacattcagc agcgcgctca tatcgaggcg
      301 ctattgcgag acacggacga tgagcggatc atcgaggctc tcgagagcag gcacgagggg
      361 ttgtttgtga aaaacctcga gaacgtgcag ggagacgagc gggatgtgat tctcttttcg
      421 acggcgttca gtgtgaatga caagggcgta ttgccgctgc aatttggccc gttgaatcgt
      481 caaggggggg agcgacggtt gaatgtggcg gtaacgcggg cgcgtcgtca ggtgattgtt
      541 ttcagttcgt tcgatccgtc gcagcttcgg gcggaggaga catcgtcggt ggggattcag
      601 cttttgcgtg cctatctcga cgcggcgcag cggggggcgg atgcgatgag ggtcgccagc
      661 cgacgtcctt tggtggacca tcatcgggac gagatggcga ggcgtttggg gcaagcgggg
      721 cttgcggtgc gcaaagacgt gggaatatcg agttttcgtg tggatatcga gcttgcgttg
      781 caggaaagac cggagcagcc gtgtgttgct ttgcttctcg atggggaaag ctggtatgcg
      841 cgttcgacgg tatcggatcg ggatgggatg cccgcgctgg tgttgggcaa gatgatgcga
      901 tggccggtcg tggagcgggt gtggatgccc gagtggctgg cggatccgga agcggtgatc
      961 gcgcgattgg cgaaggcggt tcacgcccca ccggccagag cggtggagaa gaaaaggtct
     1021 caaacccttg taatgtcgac ggatttttct gcatcgaagc cagagcagcc agcgattcag
     1081 acgaccgtgg agccgtcgtt gctcggacag acgccttttc aggaagcgat tgggcagaag
     1141 gtagggaata aagagcaatt ggatgcgttg caggagtcct acggcgtgca gaagatggtt
     1201 gtagaactgg cgaatcggat gctggaggta gaggggccgg ttcatgagga gcgtttggcc
     1261 aggtttttga tggtgagttt tgggattcag caattgcaca agaagcgcaa ggatgaagtg
     1321 cttggggtgg tggagaagca tattcgaaag gagcgggatt ttttctggcc tgcggggatg
     1381 gacgaagcca cttgggtaga ttaccggccg gatccggaat ataagcgtgc gctgcatcag
     1441 atagagcttg tggaacttgg caatgcgatg gcggatcttg ggaaaaaact gggaggggga
     1501 ttcaaagaag agctattccg tcagactctg ggcgtatttg gaattcagcg gttgacagcc
     1561 caggccaggg agcgcttgga ggcatcgttg gcgttgatgg tagagcggtc gaggcttgtg
     1621 attcggggcg atgaggtgat tgaggtgttg tgaggcaagg agtgcctatt atcggagggt
     1681 gatggcccgg gactattcct tgtggtttgg tcgaccgcga gagcgcatgg ttcagggctc
     1741 cgaggcgtca acgttatccc ctgcccatcc atagttttgt acacacaacg ttatatgtta
     1801 tgagattcag gttttggatt cattcatgaa acgttgtttc tttcttttgc gactctatca
     1861 gtcctgtccc ctccaaagcc tttgccaagc atcatatagg cacaacgtta tatgcttatg
     1921 ctctctgtcg actcgtggtc ttgtccgggg caccgcttgc gtacggctcg gacaatggtt
     1981 tccatgaaca gttcaaccac aaggaaaaag ccactgtacg tcggcttcaa aatgcgtcat
     2041 gctccctttc gaatgaagac ttcccgagcc tatcgattgg gttggagctt ggtgtttggc
     2101 tgggctcttg tagctgcctg tgcaaggcca caggtcaccg ggccgcaagc gagcgagcct
     2161 ccaagcgctg tggacgacca gaagccgggc gatggccagg cattcgagcc accggctccg
     2221 aacctgatca ccacaaactc tgaagcgagc ccctcgcggg agcccgtggc cgagttcatt
     2281 cggctggttc ttcctcgtga tgatgctttt ctttcaaccc ccttttcgcc tgccaatccc
     2341 gctcgttacg cgggttggag ggagtccgga cggcttcacg ttggcaaggg aaaccttcac
     2401 tacgcagaag ccatagacac gcaacgtctt gtgttagcca tgagcacgac cgaaggcacg
     2461 gtccaagtgt atgatcgagc gacacgctcg ctgaaacacg tcgtttccgt ggcaaatctc
     2521 gatgatttcg ggcgtagcgc gatggctccg tggccggagg gcagcgctct gcacggcttt
     2581 ttggccgggc gaaaagacgg gctctggctc tatgatgcca catcgacgcg agccccccag
     2641 cgcttggacg acgcttttct ctggaccatg cggtggtctc ccgatcaaac ggtcttgctg
     2701 ggagccagtt ccgacacgaa atcgcaaacc tccgtcttgt acttttaccg tcgcacgtct
     2761 cctgacaagc tagaaagggt cggcgcatac aatctacccg aacgggtgga aggactcgat
     2821 ctgagccgtg ataatcgtca tttgctgctc tcgctatatc cctcgaatac gatggtgctg
     2881 gcggacctgc gggaagggcg gattttatgg cgggtgcctg cgccggagtt cgcctcggac
     2941 gtgagttttt cgcctgacgg ccaggcagtg gccgcgggag gggcaggagt cgtgctcctg
     3001 gatgccacac gtcccacacg ccgcacgaag tacacgggtt ttggcaataa cgtggcccgg
     3061 gtgcgttttt ctcccagtgg agacgccctt gcggtttcct cgtacgatgg gaaagtccgg
     3121 atatttagct gggatctcga cagcctctcg ctgacactca ccaaaacact atcccatacg
     3181 ggcaccgcca acgtgtatgc ccttcatttt ctcgatgatg gcgacacgct gatgtcatcg
     3241 tccggggatc agacggtgcg gttttggagc ggccctgctg gacgacaacc tttgaaaaac
     3301 ccatggcagc aaggccaatg gaaaccgctg gtggtggtgg agcccatggc cggggctggt
     3361 ggattggaaa ggccttttga ttggagcgcg ctcgaccgcg aggcgcgggc gaaacttttt
     3421 ggcgaattgc gtgctgaacc tcgtcaccgt cctgctcggc tcgatggtcc cccctccccc
     3481 tcggctatcg ttcctggaaa ttatgcatgt cgggtaggca gcctttacaa gctacgcgac
     3541 tgcatcgttc agaaggattc cgcgggtcat gtcctattgg aggtgttgga gggcaacatc
     3601 attccgatgc ggggggtgtt attcgacgat gggcctgtcg ttcgtttcga aggggcttcc
     3661 acggcggttc gaccatttgc gtgcatggct tgtcaggacc ggtgctattt gtttgatgat
     3721 agttgctctt gtcgtccgga acctcccgat cgcatccggc aatgtctctc ccagccgctt
     3781 catgccgtat ttcggaaagt gggaaaccga tggcaagggg tattggcgta tgcgctgagt
     3841 caggacgaca tcctcaaccc ggttcctttg ccggcggacg cgccgttttc ggacagtcgg
     3901 ggacgtttta cgattgaact catagcccga tgagaagaga gaaacctgaa tgacatccgc
     3961 ataagatttc ccttcgaggc ttgcggagcg gcacggtcct ttttggtgat taccaccggg
     4021 aggctatcgt tgccggaacg ggtcctgaaa gaagattttc catccatcgt tgtcaagatt
     4081 tcccgccgaa cgatactatg gcgctaacca tcgtgggatc aatgcttgcc caaagcctgc
     4141 accttattga aaagacgcag gaaattgcct ccaaaaatcg ccgccacctc tggctctgag
     4201 aatccttccg cgagcaaagt ctcgcgaagt ctgggcaact cggccgcggt ctccagtccc
     4261 gacggccctt tggagatgcc atcgaagtcc gagcccaagc ccacacagcc gatgccgccc
     4321 acatcgcgaa gatatcgaat atgctgcacc acatgggcca gagtcgcctt tccagattgg
     4381 acgagatgcg gaggataaaa attgacacct atgagtcccc ctttttcggc aatagcacga
     4441 atatgcgcat cattcaggtt ccgggggtga gggcatacgc tcctggcatt ggaatgggaa
     4501 gccacaatag gcgcttggga aatcgccaaa acatcgtcga tggcgcggtc actcatatgc
     4561 gagacatcca cgatgatacc gaggtgattc atgcgcacga cggcattttt gcctcgttcc
     4621 gtaaggccga tggggccgtg gtagacccaa cttgtggcaa aatcatggtc attttcccag
     4681 gtcaagccga tcatacgtat acctgaagca tacaggtcct cgatgcgata gatcccatgg
     4741 cgaaggcaga aagagccttc gagggtgcga aatacggaaa tatggccgga atatcggtta
     4801 gttttgaagt cttcgggggt caaggcgagg cggagtagac cagagtggtc ttcgagcaga
     4861 gcatcgattt ttttggtggc ccgttggcaa taaagggcag tgttctcgtt ttgcgatgcg
     4921 gattctacaa aaagcacggc gctaaaagca tcgatttttc cttggcgaag acgaggaaga
     4981 ctcaggtgta cggccgtttc gttcccaatg tttttacctt tcatcaattg gtacggggta
     5041 tcggcgtgca tgagcatggt aataaaagga aactcaggtt ccaa
//
LOCUS       DAOSOR010000443         5081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_16605_length_5081_cov_4.640867, whole genome shotgun sequence.
ACCESSION   DAOSOR010000443 DAOSOR010000000
VERSION     DAOSOR010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16605_length_5081_cov_4.640867"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..618)
                     /locus_tag="PK710_19190"
     CDS             complement(115..618)
                     /locus_tag="PK710_19190"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT11912.1"
                     /translation="MNIQDDCFVSIAYRLTSDSGEVLDESAADQPLSFIFGRGQMIPG
                     LEQKLEGMSAGQNAQITVEPEDGYGPIREELFRELPRDSFPDDLQLEPGMHLSASGPH
                     GPLSLKVKSIQEDTVTVDLNHPLAGQRLHFDVTIVESREATDEEIFASTMSCSPSACA
                     GCGGSCH"
     gene            622..774
                     /locus_tag="PK710_19195"
     CDS             622..774
                     /locus_tag="PK710_19195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11913.1"
                     /translation="MIPTKQWGDFGIRAWHEQGTDGRIRSVGVCTGLRSARIAHRPVG
                     CYGVGG"
     gene            complement(787..2865)
                     /locus_tag="PK710_19200"
     CDS             complement(787..2865)
                     /locus_tag="PK710_19200"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT11914.1"
                     /translation="MEGRSRVVSEPSHPGTYFIGRYRVVDDIGVGGMATVHLARMDGA
                     GGFQKWVAIKRIHPHLVENEQIIKMFLDEARIAASISHPNVAQVFDLGSFENTYWIAM
                     EYLHGEPLREVLRHVMETGDVLSGPLAARIIADAAEGLHAAHELRGKDGRPLNLVHRD
                     VTPHNLFITYAGVVKVVDFGIAKVAGRLSSTQAGTLKGKIAYMAPEQIRGKDIDRRAD
                     IFALGVVLWELTTGRRLFRMDTDLETVERVQDCVVPPPSSLTYDYPVQLESIVMTALR
                     KNREDRFQTARDLSRALQQYLLQSSQFIGPEEVSAYIGELFGDRIQQREAQLKWAADV
                     TKTLSFENFRPAFRAAGLSVDAARQQVGAVPPPPIPGQTDDDDDDDDDADETVVEPFR
                     AAIMRQEAIRYNKDKPATHDVPRPKTAQRPVVPKPSPGGQTDSITIKGKSPSLAQQRA
                     FDTKSVDSTAQNRPSPSRLRASEPSLASNDLVDDDEDDEVATIVREPAVALKQDRLFA
                     QGRPAPSAANRPAPQQPQQPQQPHHPVAPSVTPSGVVPVPSPSGFTPSGVIVARQDGI
                     PAPARMPSELNVPASGAILGAPVPGPAPSVASQSMGPVPPISQQRIPTGYEFSPAISP
                     TSNPYVIAEPGFDENQWARGLRGSPILVYAAAALGGFLVVAVLIAVLLGGPEPSPQPI
                     PLPPPSPASS"
     gene            3241..3648
                     /locus_tag="PK710_19205"
     CDS             3241..3648
                     /locus_tag="PK710_19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11915.1"
                     /translation="MQPIMEMLDRHFNVLHNRAQVMLGLCGVVITTTGFSGRIIAGTN
                     TLAQWLIVSSVALVLLAAAVVVWGVLHLRWLTQQPGDSVQVWLMEALTYRDHKTQAYR
                     IGISLLLVGLALYVAAIAIMLLHPDRNAVPLNR"
     gene            complement(3706..>5081)
                     /locus_tag="PK710_19210"
     CDS             complement(3706..>5081)
                     /locus_tag="PK710_19210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11916.1"
                     /translation="RPDDAGDINAVDEVPRSSWYQPEGAELALLDPPILQDGPPMLPL
                     TVLHDSPRIRSDGLCVLDARNYRYELRRDDSERPEMRTSAAAIASRLIRAVGLLSPEV
                     HIVFLSEQDFRWPTEDRTLHSSLRALIPPAPANADSIVRMSATRWPVGIDVGVTDPFD
                     ARDDDPNDVVPHRNRRTMRALKVLGAWLGISSINPSKTRDVYVGSPGYGHLRHYVVGM
                     EDALGAASVVKSDKKQGLRTDLGGGPGFNFLTLGLWPGSLRPVTQTRWLAIGDFDENV
                     DPADFTTSPPYAPMTHVRAADGYWAAKRIASIPTKTIVQAVRHARIHDPSAEKKLIDV
                     LLQRRHAVVKFWYSQVTPAEMVHIEARKVTLRDEAISRGLIPPTSVQYHGEIVDDSGV
                     VQSRIPRFEPATESFEIQIPRKVAETNDRIVLRLQVEVDGKRAPRVCEMHFRPVAHSL
                     RLLGIQH"
BASE COUNT         1097 a         1425 c         1476 g         1083 t
ORIGIN      
        1 cacggaagct atcacgcaac gcggattcga agccagcacg cgaggttatg ataagaaaac
       61 ggcgacaggc tcgtttcgga gatcaaggta gatggcagcc gtgcgttacg gacttcaatg
      121 gcaagagcca ccgcagccag cacatgccga agggctacaa ctcatggtcg aagcgaagat
      181 ttcctcatcc gttgcttcac gagattccac gatggtgaca tcgaaatgca gacgttgccc
      241 cgcgagcgga tgattgaggt ccacggtcac ggtgtcttct tgaatggatt tgactttgag
      301 gctcaacgga ccatgcgggc cgctggcact caagtgcatg ccaggttcca attgcagatc
      361 gtcggggaag ctatcacggg gcaattcgcg gaacagctcc tcacgaatcg ggccgtaacc
      421 atcttccggc tcgactgtga tctgcgcgtt ctgcccggca ctcattcctt cgagtttttg
      481 ctcgaggcca ggaatcatct ggcctctgcc gaaaatgaac gacaagggtt ggtcggccgc
      541 ggactcgtca agcacctcac ccgaatccga ggtcaatcga taggcaatgg acacgaaaca
      601 gtcgtcttgg atattcatgc gatgattcct acgaagcaat ggggcgactt tggcattcgc
      661 gcttggcatg agcaagggac agatggaaga attcgttccg tgggtgtttg caccggactt
      721 cggagcgctc gtatcgctca ccggcccgtg gggtgttatg gggttggcgg gtgaatatcc
      781 agtgatttac gaagaagcgg gagatggggg tggcagcggg ataggttggg gagagggttc
      841 cggcccaccg aggagcacgg caatgagcac ggcgacgaca aggaatcctc ccagggccgc
      901 cgccgcatac acgaggatag gggaaccacg cagcccccgt gcccattggt tttcgtcaaa
      961 cccaggttcg gcgatgacat aagggttgct ggtcggcgat attgctggtg aaaactcgta
     1021 tcccgtgggg attcgttgtt gagaaatggg aggaacgggg cccatgcttt gggaagcgac
     1081 actcggagca ggaccaggca caggggctcc aaggatcgct cctgaagcgg ggacattgag
     1141 ttcggaaggc atccgggcag gagcagggat tccgtcttgc cgggcgacaa tgactcccga
     1201 cggggtgaat ccactgggag acggaacggg gacaacaccg gaaggggtca cgctgggcgc
     1261 gaccggatgg tggggctgtt gtggttgctg aggttgctgt ggtgcggggc ggtttgcggc
     1321 cgatggcgct ggtctacctt gagcaaaaag ccggtcctgt ttgagcgcca ctgcgggctc
     1381 acgcacgatc gtggccacct catcatcttc gtcgtcatcc accaaatcat tggaggcgag
     1441 gctgggctcg gaagcgcgta gccgcgacgg cgacgggcgg ttttgggccg tgctgtcgac
     1501 ggatttggta tcgaacgcgc gctgctgagc caacgaagga ctttttcctt tgatggttat
     1561 ggagtcggtt tggcctccgg gggatggttt gggaacgacg ggtcgttgtg cggttttggg
     1621 ccgaggcacg tcgtgagttg cgggtttgtc cttgttgtac cggatggcct cttgccgcat
     1681 gatggcggcg cgaaaaggtt ctacgactgt ctcgtccgca tcgtcatcgt catcgtcatc
     1741 gtcatcggtt tggccgggaa ttggaggcgg cgggacggct cccacttgtt gtcgcgcggc
     1801 gtcgaccgac agtccagcag cgcggaaagc aggacggaag ttttcaaaag aaagcgtttt
     1861 ggtaacgtcc gctgcccact tgagttgcgc ttcccgctgc tgaattcggt cgccaaaaag
     1921 ttctccgata tacgcactta cttcttcggg accgatgaat tgggaggatt gcaacaagta
     1981 ttgctgcaac gcgcgcgaca gatctcgggc cgtctgaaac cggtcctcac ggttcttgcg
     2041 aagagccgtc atcacgatgg actcgagttg cacgggatag tcgtacgtga gcgaagaagg
     2101 cggaggaacg acacaatcct gaacccgctc gaccgtttcc aaatccgtat ccatccgaaa
     2161 cagacgtcgc ccggtggtaa gctcccataa caccaccccc agcgcgaaga tatcggccct
     2221 tcggtcgata tctttgcccc ggatctgctc gggagccatg taggcaatct tgcctttcag
     2281 ggtgccagct tgggtcgaag acaagcgtcc agccaccttg gcaatcccaa agtccacgac
     2341 tttcacgacc cccgcatagg tgatgaatag attgtgcggg gtgacatcgc gatggaccaa
     2401 gttgaggggc ctgccatctt ttccgcgcag ttcgtgagcg gcgtgaagcc cttcggccgc
     2461 gtccgcaatg atacgggcag ccagaggccc actgagaaca tcccccgttt ccatgacgtg
     2521 ccgcaacact tctcgaagag gctcgccgtg taggtattcc atggcgatcc aatacgtgtt
     2581 ttcaaagctg cctaggtcga agacctgagc cacgttcgga tgcgaaatac tcgcggcaat
     2641 tcgcgcttcg tcgagaaaca tcttgatgat ttgttcattc tcgaccaggt gcggatgaat
     2701 gcgtttgatc gccacccact tctgaaagcc ccccgcgcca tccattcgcg ccaaatgaac
     2761 ggtagccatg cccccaacgc cgatatcatc gacgacacga tagcgcccga tgaagtatgt
     2821 cccagggtgt gagggctctg acacaacgcg gctgcgccct tccatacctg gcggactagg
     2881 cggtggcttc ggggacacgg tacggctcat ggaaacgagg tcgacaaacg gccgttcatg
     2941 acaataacac agccagagcc actgaacggt cggaaagtcg cgggcaggcc atgctcaggc
     3001 ctattgccaa tcatgccgca cgctccacga gaaagccaac ttaccgatcc cgcatgccaa
     3061 tttatctatt tttcaattaa ttacatacaa caattttatt taattatatg taatacatga
     3121 catatttgca tttaccttcg tattgctcta cgaacatgcc gttgctaacc atcgccaatg
     3181 ccatttctca gccgcaagca agaatgtgac aggatcctag ggcaaatcgg cgagggccga
     3241 atgcaaccca tcatggaaat gctggatcgt cacttcaatg tgcttcataa ccgcgcgcaa
     3301 gtcatgctgg ggctatgcgg ggtcgtcatt acgacaaccg ggttttccgg gcgaatcatc
     3361 gcaggcacca acacccttgc ccaatggctc atcgtcagta gcgtcgcgct cgtgttgctc
     3421 gctgccgccg tcgtcgtctg gggcgtgctg cacttgcgtt ggttgacgca acaacctggt
     3481 gacagtgtcc aagtctggct gatggaagcg ttgacctatc gggaccacaa aacacaagcc
     3541 taccgtatcg gtatttcgct gctgctcgtc gggttggcac tctacgtcgc agccatcgcc
     3601 atcatgcttc tccatcccga tcgaaacgcc gtccccctca atcgttgacc gtattggcac
     3661 gagtcccagt acttcgaccc accgccggcc ggcaacgatt ttgcctcaat gctgtatgcc
     3721 gagcagacga agggagtggg caacgggacg aaaatgcatt tcacaaacgc gcggcgctcg
     3781 ctttccgtcg acttcaacct gcagacgcag cacgatccta tcgttcgttt cagcgacctt
     3841 tcgtgggatt tgaatttcga acgattccgt agcgggttcg aatcgtggaa tccgactttg
     3901 tacgaccccg ctatcatcga caatttcccc gtgatactgg acggacgtcg gcgggatcaa
     3961 tccacggctg atggcctcgt cacgcagtgt cactttcctt gcttcgatat gaaccatctc
     4021 ggcaggcgtg acttgtgaat accagaattt cacgaccgca tgacgacgtt gcaacagcac
     4081 gtcgatcaat tttttttccg cgctcgggtc atgaatccgg gcatgccgga ctgcttgaac
     4141 aatggttttc gtaggaatcg acgcgatgcg tttggcagcc caatagccgt cggcagcccg
     4201 cacatgggtc atgggtgcat aaggcggtga agtcgtgaaa tccgcggggt cgacgttttc
     4261 gtcgaagtcc ccaatcgcta gccatctcgt ttgcgtcacg gggcgcaaag aaccgggcca
     4321 caatcccaaa gtgaggaagt tgaagcccgg acctcccccc aaatcagttc taagtccctg
     4381 ttttttatct gatttaacta cactcgccgc cccaagggca tcctccatcc ccacgacata
     4441 atgacgaaga tgaccatacc ccgggcttcc gacatagacg tctcgcgtct tgcttggatt
     4501 gatggagctg ataccaagcc aagcacccag cactttcaaa gcgcgcatgg ttcgacgatt
     4561 tctgtgcgga accacgtcgt tgggatcgtc atctcgagca tcgaacggat ccgtcacccc
     4621 cacatcgatg cccacgggcc atcgtgtggc gctcatacga acgatactgt cagcgttcgc
     4681 gggggccgga ggaatcaacg cgcgcaacga cgagtgcagc gttcgatcct cggtcggcca
     4741 tctgaaatcc tgttccgaaa ggaaaacgat atgcacttcc ggtgacaaca agccgacagc
     4801 gcgaatcaat cggctggcga tcgcggcggc agaggttcgc atttcaggac gttcggaatc
     4861 gtcacggcga agttcgtagc gatagttgcg ggcatccaag acgcacagcc catcgctgcg
     4921 aatccgagga gaatcatgca acaccgtgag gggaagcatc ggcggtccat cttgcaggat
     4981 cggcggatcg agcagcgcaa gttccgcccc ttccggttga taccaactcg atcgtgggac
     5041 ctcgtccacc gcgttgatat ctcccgcgtc gtcgggacgg t
//
LOCUS       DAOSOR010000444         5060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_16681_length_5060_cov_3.808791, whole genome shotgun sequence.
ACCESSION   DAOSOR010000444 DAOSOR010000000
VERSION     DAOSOR010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16681_length_5060_cov_3.808791"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1094
                     /locus_tag="PK710_19215"
     CDS             156..1094
                     /locus_tag="PK710_19215"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOT11917.1"
                     /translation="MRVAIFIDGKNFYAGWKDRAGGRRIDFGAMARWIVKRAEGHALW
                     GAHYYTGVETGPASGSESQAKLNGFLDMLEMQPGFFVERFPRKANTFQCVVCGSENRY
                     TQEKEVDTTMVADMLRYAAVGAYDILVLLSGDADYAPAMEGVRAIGKQAYVATWGGAG
                     LASRIRRAAFDHIDLLEGLSMFGRNDDGIPPSVPYVDTYDPPITLRGGDQSLESLRIR
                     EGESVEGLDQSRFLEEIRRAEEKFMGGYVGANYFVTRWASDGLGETAELRRRMLDVLV
                     EEGKVEIYDAPDGNKAIRLASSTNEQEQGRNIDEEP"
     gene            1253..1897
                     /locus_tag="PK710_19220"
     CDS             1253..1897
                     /locus_tag="PK710_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT11918.1"
                     /translation="MASSQSSASTLSEAVLLPCAYSEQQLLDEMLGGRDHAWVEFHNR
                     YARLIFRCITKVTGRFSTVVSAEDVQEIYATLILQLLSNDMHKLRSYDPQRGNRLGTW
                     IGMLAINASYDHLRAARREPNRGSLVEAEEMTSDIMDPFAEVDRKQREQIVADLLESF
                     SEKDRLFVALYFDQGLDAEEVALRMKISVKTVYSKKHKIRARLEKLLQRDLLAA"
     gene            1986..2129
                     /locus_tag="PK710_19225"
     CDS             1986..2129
                     /locus_tag="PK710_19225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11919.1"
                     /translation="MANYKLWNMRLRHEWLKSPKKPKRLKRCQRVRVGLQAAIDRKAP
                     KEA"
     gene            complement(2160..2876)
                     /locus_tag="PK710_19230"
     CDS             complement(2160..2876)
                     /locus_tag="PK710_19230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11920.1"
                     /translation="MTPYDVHTLVRSLLLGSTLCLLTFVVMVASDGGHTTVAQKIGQL
                     CVLTPLLAALGARIAMMQAQSRGETKALESLGASPSRVGLGASLAVVVLGAMATAALA
                     ARVGDIEGLLPRLDGVSWTQLPEGVWISTDSSMKVDAQGLPSFGSFDRQTESISRSTT
                     PFFGVVAAMTVALSDWSRERIGTWSRMMTVLVGSGLAILVFHLVAADRASPWLLLVVP
                     LPIIAQTWHIRLYRWATLGR"
     gene            2927..3484
                     /locus_tag="PK710_19235"
     CDS             2927..3484
                     /locus_tag="PK710_19235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11921.1"
                     /translation="MRQRFFVSWLMVGAMGISNAGCMADPDDGRMPVPGEYLGTYGVN
                     AALLDTSCGAGALSPPGQWDFKVLLSKDGRHLYWTNGAESISGDLASDGKAFSFKTEV
                     SLQLSEPKASRAGCVVWRQDTAEGALEFEEKSSVYPGFSGKLSYSYALGAGSECGDLL
                     DTSGIPTLPCQIRFEMTARREPDKD"
     gene            complement(3481..4116)
                     /locus_tag="PK710_19240"
     CDS             complement(3481..4116)
                     /locus_tag="PK710_19240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11922.1"
                     /translation="MNRYVSWLWLALACCTLFGCQRCSGNPYQGDAILADAAAAPSLE
                     PTDQPIPEAFQKDALWNQAMQSEDDIDLASLADREGASGLLDALEVGGKVGQTALAAL
                     PMADDAPVAFRRLSQLVLLTQGSAQQRVMQTIFSIAETPIHRGEPIDDGGAELCAMAL
                     TRLVQDSKTPAPLRSLAISTLRLPAFVVFVDASTNPMTPGAPPGSASVDGP"
     gene            4222..>5060
                     /locus_tag="PK710_19245"
     CDS             4222..>5060
                     /locus_tag="PK710_19245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11923.1"
                     /translation="MVAVRWFAMLAVGCLYGCVEDSNDRAVKQQANTAEEQAEEKQKA
                     QRQTDREECRRLRHRLEQYPALAGTPRLDEQRHRVLGQAKGWPVVFRREPIRDEEELS
                     PYFRAISEAYTDRRRSFSAFETLRGSVQHHRKQVRQILMPEGYLYADDPEVARWLVTH
                     LDLRRLFNEPELWLMRGDEVFRLERTERGYRHVDGANAGAAASLLLFDRVTTRRSELE
                     PVLHVDFVRAAEQLGFDRVEIERLTSEGINARLRYGSDSLWVQAVFSEQQGRTQLVCE
                     IIE"
BASE COUNT         1145 a         1274 c         1510 g         1131 t
ORIGIN      
        1 cttgcattgt aagctacacg gccgcaagcg tcgatgctgg aggcatggtg gatgcggcgg
       61 atgtgaaaac gcaattcaaa gcgtgacaat cgaaaaacac gcgagtaatc ggaagttgtg
      121 gtgaagactt tttggtagac gatgggttga tgatcatgcg cgttgcaata ttcattgatg
      181 gaaaaaattt ttacgcgggt tggaaagatc gggcaggggg gaggcgaatc gattttgggg
      241 cgatggcgcg gtggatcgtc aaacgtgccg aggggcatgc gctttggggg gcgcattatt
      301 atacgggcgt ggagaccgga cccgcttccg gttccgaaag tcaggcaaag ctcaacggat
      361 ttctcgacat gctcgagatg cagcctggat ttttcgtgga acgttttccc cgcaaagcca
      421 atacttttca gtgtgtggtg tgcggaagcg aaaatcgata cacccaggag aaggaagtcg
      481 ataccacgat ggtggcggat atgcttcgct acgcggcggt tggggcctac gacatcctcg
      541 tgctgttatc gggggacgcg gactatgccc cggcgatgga aggcgtgcgt gccattggaa
      601 agcaagccta cgtcgccacg tggggaggag ctgggctggc gtcgaggata cgaagagctg
      661 ctttcgatca tatcgatttg ctcgaaggtt tatcgatgtt cgggcgaaac gacgatggga
      721 ttcctccttc ggtcccctat gtcgacacgt atgatccccc cattaccctt cggggaggag
      781 accaatcgct ggaatcgctg agaatacgtg agggtgaatc cgtggagggg ctggatcaat
      841 cgcgttttct tgaagaaatc cgacgggcgg aggagaaatt tatgggcggg tacgtgggtg
      901 cgaattattt cgtcacgcgt tgggcctccg acgggctcgg agagaccgcg gagttgcgta
      961 gacgtatgct cgatgtgctt gtggaagaag ggaaagttga aatttatgat gcgccggatg
     1021 gcaacaaggc gattcggctg gcttcttcga caaatgagca agagcaagga aggaatatcg
     1081 acgaggaacc gtaatatgat tccgtaaccg gtgttgcgcg taacgttacg ccgacatggg
     1141 gtgaatgtta cgctaccgtt tcctgctcat ctcgttgttt tcgcggagtt gaacgcaatc
     1201 tatgcctggc atgaagcctg cactctgacc ctcccaccgg aggttcatgt tgatggcttc
     1261 ttcccaatcg agcgcttcca ccctatccga ggcggttctt cttccttgtg cttattcaga
     1321 acagcagctc ctcgacgaga tgctcggtgg ccgtgaccat gcctgggtgg agtttcacaa
     1381 ccgttacgcc cgcttgattt tccgttgtat caccaaagtc acgggacgct tttccaccgt
     1441 cgtcagcgcg gaagatgtgc aagagattta cgctaccctc attcttcagc ttctttcgaa
     1501 cgatatgcat aaactccggt cttacgatcc gcagcggggg aaccgtttag gaacttggat
     1561 tggaatgctt gccatcaacg catcttatga ccatttgcga gccgcgaggc gtgagcccaa
     1621 ccgcgggtcg ctggtagagg cagaagagat gacgagcgac atcatggatc cgtttgcgga
     1681 agtagatcga aaacagcgtg agcagatagt tgcggacctg ctcgagagct tctctgagaa
     1741 agaccggctt tttgttgctc tgtatttcga tcaagggttg gatgcggaag aagtcgccct
     1801 gcgcatgaaa atttctgtca aaacggtcta ttcgaagaaa cacaagatcc gggcgcggtt
     1861 ggagaagttg cttcaacgcg atctgcttgc cgcctgacct cgagcctctc tttttctgcg
     1921 gatatcacct tgaccaaggc atggctctcg ggcaaactgc gccgccgtac ggagacaata
     1981 gactcatggc gaattataaa ctgtggaata tgcgacttcg acacgagtgg ctcaagagtc
     2041 ccaaaaaacc aaagcgtctc aagcgctgtc agcgggtgcg tgttggattg caagctgcca
     2101 tcgaccgaaa agctcccaaa gaagcctgat cttttcgtat tcccgattcc ttcccctcgt
     2161 tatcgaccta gcgtggccca tcggtagagt cggatatgcc aggtttgtgc gataatcggc
     2221 aaaggcacaa caagaagaag ccaaggagag gctctatccg cggctactag gtgaaatacg
     2281 aggatcgcca aaccgcttcc gacaaggacc gtcatcatcc gcgaccaagt gccgattcgt
     2341 tcccgggacc aatccgaaag cgctaccgtc atcgctgcca cgactccgaa aaacggcgtc
     2401 gttgagcgag aaattgactc ggtttgccgg tcgaaggaac caaacgaagg cagtccctgc
     2461 gcatccactt tcatcgaaga atccgtggaa atccaaacac cttcgggcag ttgggtccac
     2521 gataccccat ccaaacgcgg caataaccct tctatatccc ctacccttgc tgccaaggcc
     2581 gcggttgcca tggcaccgag aacaacaacg gccagggaag ctcccaaccc aaccctcgaa
     2641 ggagaggctc cgaggctttc aagggctttg gtctcacctc gcgactgcgc ttgcatcatg
     2701 gcaatccgtg ctcccagtgc cgccaacaaa ggtgtgagaa cgcataattg cccgattttt
     2761 tgcgctaccg tggtgtgtcc cccgtcggac gctaccatca cgacgaatgt caacaggcat
     2821 agggtcgaac ccaaaaggag gcttcgaacc agcgtgtgta cgtcataagg agtcatcgaa
     2881 ctccgtgaac ttggcagatt ccgacgtctt caggtaggtc caaatcatgc ggcaacggtt
     2941 cttcgtttca tggctgatgg tgggtgccat gggtatatcg aacgctggtt gtatggctga
     3001 ccctgacgac ggtcgcatgc ccgtgcctgg tgagtatctt gggacttatg gtgtcaatgc
     3061 ggccttgttg gacacatcat gcggtgctgg agccctatcg ccgcctgggc aatgggactt
     3121 caaggtgctt ttgtcaaaag atgggaggca tttatactgg accaacggtg cggagtcgat
     3181 ttctggcgac ctcgcttcgg acggcaaggc attttctttc aaaacggaag tatccctcca
     3241 actatccgag cccaaagcca gtcgcgctgg atgcgtcgtg tggcggcagg atacggcgga
     3301 aggggcattg gaattcgaag aaaaaagcag tgtctatcca ggtttttccg gaaagctctc
     3361 ctattcgtat gccttgggtg ctggatcgga atgcggggac ctgctcgata cctctggcat
     3421 acctaccctt ccttgccaga ttcgcttcga aatgacagct cgtcgggagc cagacaagga
     3481 ttaaggtccg tcaacgctgg cagatcccgg tggagcaccg ggggtcatgg ggttggtgga
     3541 agcatcgaca aagacaacaa atgccggtag acggagagta gaaatagcga gcgagcgcag
     3601 gggggcagga gttttcgaat cttgaacgag gcgtgtcagg gccatggcgc aaagctcggc
     3661 gcctccatca tcgatgggtt cacctcgatg gatcggtgtt tcggcaatgc tgaaaatggt
     3721 ctgcattacc cgttgttggg ccgagccttg ggtaagcaat acgagttggg agagacgccg
     3781 gaacgccacg ggggcatcgt ctgccatggg gagagctgca agcgctgttt gcccaacttt
     3841 gccaccaact tccaacgcat cgagcagacc ggaggctcct tctcgatctg caaggctagc
     3901 tagatcgata tcatcctcgc tttgcatggc ttgattccac agcgcatcct tttggaaagc
     3961 ctcgggaatc ggttgatccg tgggctcgag agaaggggca gcagcagcgt cggcgagaat
     4021 ggcatcccct tgatacgggt tgccggagca gcgttggcat ccgaacagag tgcagcatgc
     4081 cagagcgagc catagccatg aaacgtatcg attcacgcgc gggcatgtag catgaaaccg
     4141 gagtaatcgc gagcgcgcaa gcgtaggcca tgcaagcctt gcttggcctt ctgcctcgtg
     4201 tcgcctatgt tgagaccggt catggttgct gttcgttggt ttgccatgct tgcggttggc
     4261 tgcttgtacg gctgcgtcga ggactccaac gatcgcgcgg tgaaacagca ggccaacaca
     4321 gccgaagagc aagccgaaga aaagcaaaaa gctcagcgtc aaaccgatcg cgaagaatgt
     4381 cgtcgattgc gtcatcgatt ggagcagtat ccagcgcttg caggaacgcc tcgactcgat
     4441 gagcaacgtc accgtgtatt gggtcaggcg aaaggttggc cggtcgtatt tcgacgcgag
     4501 ccgattcgcg acgaggaaga gctatccccg tatttccgtg ctatttcgga ggcttacacc
     4561 gatcgtcgca ggtctttttc ggctttcgaa accttaagag ggagtgtgca gcatcatcgc
     4621 aagcaagtgc ggcagatatt gatgcccgaa gggtatttat atgccgacga tccggaggtg
     4681 gcacgttggt tggtgacgca tctggatctt cgacggttgt tcaacgagcc agaactctgg
     4741 ctcatgcgtg gagacgaggt atttcgcctc gagcgcacgg aacgcggata ccgccatgtg
     4801 gacggtgcga acgctggcgc tgcggcttcg ctgttgttat tcgatcgggt aacgacacga
     4861 aggtcggagc ttgaacctgt attgcatgtt gatttcgttc gggcggcgga acagcttggt
     4921 tttgacaggg tggagatcga gcgacttact agcgagggaa tcaatgctcg ccttcggtat
     4981 ggttccgaca gcctttgggt acaagcggtg ttttccgaac agcaaggccg tacccagctc
     5041 gtttgcgaaa tcatcgagga
//
LOCUS       DAOSOR010000445         5019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_16858_length_5019_cov_3.120266, whole genome shotgun sequence.
ACCESSION   DAOSOR010000445 DAOSOR010000000
VERSION     DAOSOR010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16858_length_5019_cov_3.120266"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(861..2522)
                     /locus_tag="PK710_19250"
     CDS             complement(861..2522)
                     /locus_tag="PK710_19250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11924.1"
                     /translation="MHDRFAQPAPGHRDWYAFEAMRSLPHLLTLVDRNPLSPTFGCFD
                     REYWHYRTVDFPCGMSQEMCLPLALAWAHAFPGNPYHRNERLRELAIAGIEFAKKSSH
                     SDGSCDDYFPYEQALGALVFSTWAMAETYRILAPDRPDLLDFLRLRGDWLREHNETGQ
                     LANHQAYAALALRAVFEITGDAVYEQASKQFQQLALSWQNEEGWFQEYEGADPGYHTT
                     TITLLAKLYRKTGDESLKEPLLRAIEFAAHFMHPDGSYAGEYGSRNTYHFYSHGFELM
                     APYSDTAARIAQTYLQRSLPERRRYFNDDDRMCAHYVYDWMLSWLDYHPERKGLLEEH
                     REPFTRWFPQAKLLVKKTPRYYAVVSMAKGGVIKVFDEHGPLMSDTGVMVQVDGQRVL
                     VSHLVGRYGQAVDVERGLLSVSGNMAWRKHKVSSPAKQIAFRSVNMTVGRLSANALRA
                     TLQKVLITGKPWSDARFHRTIELLEDDVVVRDTIDLGGKEMEVTRVMVGSDATSIYVA
                     NSNTYQESVLLPWREYPMAAESLRAQGRATLPERRLGEQKLSEDE"
     gene            complement(2515..3492)
                     /locus_tag="PK710_19255"
     CDS             complement(2515..3492)
                     /locus_tag="PK710_19255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOT11925.1"
                     /translation="MSEGASFPRKILDWRLVPLLLVTAIILWLLLHHIAGVDAFAKAV
                     SGARWGLVWAAVGVFVVDLVLSIVRWQIILWAMSKRVGFFRCAHAVLATWPLAVIIPA
                     RAGDVLRAGVIRKQVPFLEGAGSVVAEKVLDIQSLCLLSMIGSVLYGRTSWTAIAAGV
                     LVTEWLTIVLLLRYGESIDRTPLSRFAPKVQRLLVALRALLRSPRHLLGVMASSLAAW
                     VMASTMVHLLLLATGFELPYGVTLSLWPLAIFVGMLPITVAGMGTRDAAFLLAVRASV
                     GTVVRDESVVAATLGYSLIATWLLVIVGLPFALRLTFSLTQSRERNHHA"
     gene            complement(3489..4442)
                     /locus_tag="PK710_19260"
     CDS             complement(3489..4442)
                     /locus_tag="PK710_19260"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT11926.1"
                     /translation="MESTKPLDGQLEGQLGGDTMERPSADKEGVSLVIPAHNERNGAP
                     PVMKETLELLHRELDGKVPFEVIVVDDGSTDDTFEVLRPFEAQGIQVVRHRRNKGYGA
                     AIKTGIRHARFDWIMITDADGTYPAEHIPELLAAREPESMVVGARIGEHTYVPWLRRP
                     PKWVLRKLAELLSGHEIPDLNSGLRVFRREAANRFRAILPDKFSYTTTITLAMFAAGY
                     QVTFLPINYRKREGRSKIRPIADTLGFLKLIIRTVLFFDPLRIFLPLALVFFLSSVGV
                     GVGGYLWTERVMDVTTVLLMVTGIQMLVLGMIADMLNRRMS"
     gene            4745..>5019
                     /locus_tag="PK710_19265"
     CDS             4745..>5019
                     /locus_tag="PK710_19265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11927.1"
                     /translation="MALSLLRSAFSRIVSFVRHRVIAAPTHPRRIPWRIALAWVFSFA
                     SIVDILLGQSPPIASVIAAAGLGLGMGAIFGSMIDGMSYALFKLPPW"
BASE COUNT         1191 a         1606 c         1234 g          988 t
ORIGIN      
        1 ccagaagagt gccagaagag tgcaagaaaa cgcaaaatcg gctgctgggc ctcttatgtc
       61 acgatcacgc ggtcggtgac aaggtcgttc ggtccgatcg gaagcagcgt attatgccgt
      121 cgaagcactg tccaagcacg aaaaggaaca cgtttttggg ttatcgacga caccggattt
      181 ctgaaacagg gtcgggattc ccaatgagca agccgtcaag caatggggag tcgagcaggt
      241 agcgtttagc aaaggtcgtg gtctgacggt ggagccgatg accggcaccg tgcgcaacta
      301 gcggaaactg cgcaactttc tagcaggaat cgaagccacg atctcgtggt agaagcgatg
      361 ttatgggcag cggcactgcc cttggcgtcg cccgttgtcg ttcaagtcga acgtgcttgc
      421 ttcggttgta tcggccaacg tgccggtaat cgcgagaaag cgcattgagg ttgcctcgcg
      481 aagcgatgct ttggcggcct gagcggggcg ctgcaggagt taacgagcag ggtctctgaa
      541 tagggcggcc aggttcccgt gtgtccgcaa gttgctgttt taggagtagt ttttcgtaag
      601 ttccaggatc ctgagggagt tgttgaagct cgagccctgt gtatcacgct aagaccacga
      661 taatcgctgc cacaccaccg aaaccgttcc ccaaaacggc ctttctggga tgggaactag
      721 tatactcggt tcccgtcacc ttctcgtttg ctcctccggt cgtgttcgcg tcccccacaa
      781 cagccccccc cgccgatggc cctgcttttg ccagaaccgc cttcgcgaca aggaccatcc
      841 cagtcggctg aaaacaaccc tcactcatcc tccgacagct tctgctctcc cagcctccgc
      901 tccggaagcg tagcccttcc ctgcgcgcgt aacgactcgg ccgccatcgg atactctcgc
      961 cacggcaaca acaccgactc ctgatacgta ttcgaattcg caacataaat cgatgtggca
     1021 tccgacccca ccatcacccg cgtgacttcc atctccttcc cccccaaatc aatcgtgtct
     1081 cgcaccacca catcgtcttc caacagctcg atcgtgcgat gaaaacgcgc gtcagaccac
     1141 ggttttcccg taatgagaac cttctgcagc gtcgcccgaa gcgcgttggc actcagccgc
     1201 cccaccgtca tattcaccga gcgaaacgcg atctgtttgg ccggcgacga taccttgtgt
     1261 tttcgccaag ccatgtttcc agacaccgat aacaaccccc gttccacatc cacggcctgc
     1321 ccatatcgcc ccaccagatg gctcacgagc actcgttgtc catcgacttg caccatcact
     1381 cccgtatcac tcatcagcgg cccatgctcg tcgaatacct tgatcactcc ccctttcgcc
     1441 atcgatacga cagcatagta tcgcggtgtt ttcttcacca atagcttggc ttgcggaaac
     1501 catcgcgtaa acggctcgcg atgctcctcg agcaatccct ttcgctccgg atgataatcc
     1561 agccacgaca gcatccaatc gtaaacgtag tgcgcgcaca tccgatcatc gtcgttgaag
     1621 tagcgacgac gctccggcaa ggatcgctgc agatacgttt gcgcgatccg cgcggcagta
     1681 tccgaatacg gtgccatgag ttcaaaaccg tgcgaataaa agtgataggt attccggctg
     1741 ccgtactccc ccgcatacga tccatccgga tgcatgaaat gagctgcaaa ctcgatggct
     1801 cggagcaagg gctccttcag cgattcatcc cccgtttttc gatatagctt cgccaacaac
     1861 gtgatcgtcg tggtgtgata tcccggatcc gcaccctcgt attcttgaaa ccacccttct
     1921 tcattttgcc aggataacgc aagctgttga aattgcttcg acgcttgctc ataaaccgcg
     1981 tcaccggtga tctcgaacac ggcccgaagc gcaagcgccg cgtaagcttg atggttcgcg
     2041 agctgacccg tctcgttatg ctcgcgcagc caatctcccc gaaggcgcaa aaaatccagt
     2101 aaatcaggac gatccggggc caggatccga tacgtctccg ccatcgccca ggtcgagaac
     2161 acaagcgccc caagcgcctg ttcatacgga aaataatcat cgcaagagcc atccgaatgc
     2221 gatgacttct tggcgaattc gatgcccgcg atcgcaagct cccgaagacg ctcatttcga
     2281 tgatacggat tgccgggaaa cgcatgcgcc cacgccaacg ccagcggcaa acacatctcc
     2341 tggctcatcc cgcacggaaa gtcgaccgtc cgataatgcc aatactcacg gtcgaaacaa
     2401 ccaaaggtcg gcgacaacgg attgcgatcc accagggtaa gcagatgagg aagcgaacgc
     2461 atcgcttcaa aggcatacca atcacggtgt cccggcgcag gctgggcaaa gcgatcatgc
     2521 atggtgattc ctctcacgag attgtgtcag agaaaatgtc aaacgaagag caaaaggtaa
     2581 ccccacgatc acgagcaacc atgtggcgat gagagaatat ccaagggttg cggccaccac
     2641 ggactcgtcg cgtacgaccg tgcccacact cgctcgcacg gcaagcaaaa acgccgcatc
     2701 ccgcgttccc atgccagcca ccgtgatcgg aagcattccc acgaaaatgg ccagcggcca
     2761 caacgacagc gtcaccccgt aaggaagctc gaaccccgta gccaacagca acaaatgaac
     2821 catcgtgctc gccatcaccc acgcggcaag agaacttgcc atgacaccca aaaggtgccg
     2881 cggagaacga aggagcgcgc gcagagccac gagaagacgc tgcactttcg gcgcaaaccg
     2941 agaaagcggc gtcctgtcga tcgactcgcc ataccgcaaa agcaacacaa tggtcagcca
     3001 ctccgttacg agcaccccag cagcaatcgc cgtccacgaa gtacgtccgt agagcacgga
     3061 accaatcatc gaaagcaagc ataagctctg gatatctaac actttttcag ccactacgct
     3121 gcccgctccc tcgagaaacg gcacctgctt tcgaatgact cccgctcgca aaacatcccc
     3181 cgctcgcgcc ggaatgatca ccgccaaagg ccacgtcgcc aacaccgcat gcgcgcaacg
     3241 aaagaaccca acccgcttgg acatcgccca taaaatgatc tgccaacgca cgatcgacaa
     3301 aaccaggtcg accacgaata ccccaaccgc cgcccatacc aggccccaac gagccccgga
     3361 tacagccttc gcaaacgcat ccactccggc aatatggtga agcaataacc acaaaataat
     3421 cgcggtgacc aacaacaacg gcaccaggcg ccagtcgaga atttttcggg gaaaggaggc
     3481 gccttcgctc atgacatgcg acgattcaac atatccgcga tcatgcccaa gacgagcatc
     3541 tgaatccccg tcaccatgag cagcaccgtg gtcacatcca tcacccgctc cgtccacaaa
     3601 taacctccca cgccaacccc taccgacgat aggaaaaaca cgagagcaag cggcaaaaag
     3661 attcgcaacg gatcgaaaaa tagaaccgtg cgaatgatga gtttgagaaa cccgagcgtg
     3721 tccgctatcg ggcggatctt cgatcgcccc tcccgctttc ggtagttgat cggcaaaaag
     3781 gtgacttgat agcccgcggc aaacatcgca agcgtgatcg tagtcgtata cgaaaacttg
     3841 tccggcaaga tggctcggaa gcggttcgcg gcctcccgac gaaatacgcg aagaccactg
     3901 ttcaaatcgg gaatctcatg accgctgagc agttccgcaa gcttgcgaag cacccacttg
     3961 ggaggacgtc gaagccacgg cacatacgta tgctctccaa tgcgtgcccc caccaccata
     4021 ctctccggct ctcttgccgc caaaagctca ggaatatgct cggcaggata cgtgccgtcc
     4081 gcatccgtga tcatgatcca atcgaaacga gcatgacgaa tccccgtctt gatcgccgcc
     4141 ccataaccct tgttgcgacg atgacgaacg acttgaatgc cctgcgcctc gaaaggacgt
     4201 agcacctcga acgtatcgtc ggtcgagcca tcatccacca cgatcacctc gaaaggaacc
     4261 ttgccatcca actcgcgatg gaggagttca agcgtttcct tcatcaccgg cggcgcgcca
     4321 tttcgctcat tgtgggcggg aatgacaagc gatacccctt ctttatccgc cgacggacgc
     4381 tccatcgtgt caccaccaag ctgcccttcc agttgtccgt cgagaggctt cgtcgattcc
     4441 atgctgattc caaagtaggt tgtgactcac gccaggacat cgtgcgaggc attcatcatg
     4501 ttcgcgtccg cgtgtctagc ctgattccgc cacgacgtcg agccatgggc acgcggcttt
     4561 gcgtgagaaa ccatggtcat gatgactcat ggagcgtggg acgatgaaca tacctgtcga
     4621 tcgccttcag gctttccaca tcgtgcccgc acccgcggac tcgtcgtcac tcccatgatg
     4681 ttccttcgtt cgttgcgcat ttcggctgca agaggtattc aaatacgtcc aatccagaag
     4741 cgaaatggct ctctccctcc ttcgttccgc gttttcacgc atcgtttctt tcgttcgtca
     4801 tcgcgtcatc gccgcgccaa ctcaccctcg tcgcattcct tggcgcatcg ccttggcttg
     4861 ggtttttagc ttcgcttcca tcgtcgatat tctgttgggg caatcgcctc cgatcgcgtc
     4921 ggtcatcgcc gccgcgggac tcggattggg catgggcgcc atcttcggca gcatgatcga
     4981 tgggatgtcg tatgcccttt tcaagcttcc cccctggat
//
LOCUS       DAOSOR010000446         5015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_16872_length_5015_cov_3.316734, whole genome shotgun sequence.
ACCESSION   DAOSOR010000446 DAOSOR010000000
VERSION     DAOSOR010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16872_length_5015_cov_3.316734"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(325..1035)
                     /locus_tag="PK710_19270"
     CDS             complement(325..1035)
                     /locus_tag="PK710_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT11928.1"
                     /translation="MIQPPTRQETVLVIEDDPTLRMGLRATLRSEGFRVIVANDGPSG
                     LEAALSERPDLVLLDVMLPGMNGFEVLETIRQRNADLPILMLTAKGQEEDRVRGLRLG
                     ADDYIVKPFGIAELIARVEANLRRIRREEAQSATVRIGDVTIDLSSHRAQRDGQAITL
                     TALEVRLLRYFLSRDGRVLSRQQILDAVWGADYYGTDRTVDNFINRLRGIIEPNAKTP
                     RFLVTVRGAGYRFSLTGK"
     gene            complement(1032..2819)
                     /locus_tag="PK710_19275"
     CDS             complement(1032..2819)
                     /locus_tag="PK710_19275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT11929.1"
                     /translation="MRDWLRFTVIALTPALAMGWIGLRALQGQEDATRREIQVTIEAM
                     AKRMVMESQTMTRAGSNRLRDSSLPTDPVLLENALRSITPAIADAVVLSSEGELLIPA
                     RKAITSEPKGIAPPRCGTMADELARSRSSPSHGGLRDDFMHQCQEYRSVTGRWVWPLI
                     AMESFTPEHADGLASWMESHASSMRPSEREATMRASLALPTLTGPARKRVLTALSADS
                     TNHEAVRAVLPDPNPRPVLHDGATTPEIIEWNSDASIGYLKTISTGMRAGFVIHAASV
                     RRAMAHGWSTGSADYRVDLIETPAEMDRDTTPFKSVSWITPSLGVQVSLADPLLIDRR
                     TSRARIVLAGVGVGAMVVAFVLAAFLFARMRAARKLSSLRAGFVSTVSHELRTPLASL
                     RMLSELLEENRVPEDEKAEVHAALSREAKRLGETVDRLLGFSRMSAGRYSIVRKEGLV
                     FEPIHQSVMAFRDRHPDLAVEVEVERDMKAQFDDNHLRLALDNLLENAAKYAPSGTPY
                     RVVAREQNAWIVIDVIDRGPGIRRSDVKRIFEPFVRGDDRLSSAVQGSGIGLSLVRQV
                     AQAHHGHAEVMSEPGKGATFRIRFPRSES"
     gene            complement(2940..4280)
                     /locus_tag="PK710_19280"
     CDS             complement(2940..4280)
                     /locus_tag="PK710_19280"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOT11930.1"
                     /translation="MYRLSKRVLPLIVATIVTACGPQPPQSSVPRSSPQPEPAPSSVV
                     LPPTEPVAPDSPTADSTGDTMTKLATSINRLAFDLFPKLSRAQPGNLVFSPASISFAL
                     SMTWGGADGQTAKEMQQVLHFESSSSEVMEASGKLIAALTDRSRPITFRVANQLFGEK
                     TYPFEPSFLSQTARAFGAPMQQVNFKTAHEPTRRLINEWVEQQTENRIQNLIPNRGVD
                     SETRLVLVNAVYFLGDWAAPFDKQRTQPNPFHLSPSNTISVPTMSRTGSYRIASKDGV
                     TALELPYEGNDLSMIIVLPDAVDGLDAALSSMDETRWRELTNGLSHESVWVSIPSFKL
                     EPSAPMRLSTPLRELGMRTAFDRRNANFSKIANPPNPQDRLYISEVFHKGFIRTDEKG
                     TEAAAATAVTMARTASAILRPRAFKADHPFVFVVRDNETNLILFLGIVADPSKT"
     gene            4659..>5015
                     /locus_tag="PK710_19285"
     CDS             4659..>5015
                     /locus_tag="PK710_19285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOT11931.1"
                     /translation="MKSDLFRRKGLEKILAVTNDENHSLKRSLGALHVTLLGMGAIIG
                     AGIFATIGTAAAGDAARPGAGPSLMLSFVITGIVAALTAVCYAELASLVPIAGSAYTY
                     SYATLGELVAWVIGWDL"
BASE COUNT         1076 a         1359 c         1504 g         1076 t
ORIGIN      
        1 cggcctgggg ttcggcgacc agctcggtcg acacttgggc attgtcgatc tcggtgggag
       61 catcctggtt gcaaccaaat ccggcaagga tcgcgagcag aggggatgca aaacgaaacg
      121 ttcgggcgat gcgggtatgg gtcgggttca tcaatttttc ctccaggttg gggggccgaa
      181 gtggcccttc accctgcatc ctggatcgcc gatgtcttgg aattgtccct gccatgtcac
      241 ggatttgtca cggcgtgaca aacccgatgc agccctttgc catgaatcat tgccaggaat
      301 tatgacgatc gcggtgggca tgattcactt tcccgtaaga gaaaagcgat agcccgcgcc
      361 ccgtacggta acgagaaaac gaggcgtttt tgcgttcggt tcgatgatcc cgcgaagacg
      421 attgatgaaa ttatctacgg tccgatccgt gccgtaataa tccgctcccc ataccgcatc
      481 gaggatctgc tgacgcgaca gcacacgtcc atctcttgac aaaaaatacc gaagaagccg
      541 gacttcgagc gccgtgagcg tgatcgcctg gccatcgcgt tgcgctcgat gcgaagataa
      601 atcaatggtc acatcaccta tccgaaccgt ggcggattgt gcttcttcac ggcggatccg
      661 tcgcaaattc gcttcgacgc gcgcgatcaa ttcagctatc ccaaaaggtt taacgatata
      721 atcgtccgcc cccaatcgaa gtccacgaac gcgatcttct tcctgtccct ttgcggttag
      781 catcagaatc ggtagatccg cattgcgctg acgaatggtt tccagtacct cgaacccatt
      841 catgccggga agcatcacat cgagcaatac gagatccggt cgttcggata aggccgcttc
      901 cagtccagaa ggtccgtcgt tggccacaat cacccgaaat ccctcggaac gcaacgtggc
      961 gcgcaacccc atccgcagcg tgggatcgtc ttcgatgaca agcacggtct cttgtctcgt
     1021 cggtggttgt atcatgattc gctccttggg aatcgaatcc gaaaagtggc cccttttccg
     1081 ggttcgctca tgacttccgc atgaccgtga tgggcttgtg caacctgtcg cacaagggac
     1141 aatccgatac cgctgccctg aaccgcgctg ctcaatcgat cgtcgccgcg aacgaatggc
     1201 tcgaaaatac gtttcacatc cgatcgtcgg atacctgggc cgcgatcgat gacgtcgatg
     1261 acgatccacg cgttttgctc acgggctacc acccgatagg gtgtgccgga aggagcgtat
     1321 tttgccgcgt tttccagaag attatccaag gccagacgca gatgattgtc atcgaattgc
     1381 gccttcatgt ccctttccac ttcgacttcg acggccaagt ccggatgccg atctcgaaat
     1441 gccatcacgg attgatggat cggttcgaat accaaaccct cttttcgcac gatggaatac
     1501 cttccggcgc tcatccggct gaatccgagc aagcggtcca ccgtctctcc cagtcgcttc
     1561 gcttctcgcg aaagggcagc atgaacttca gccttctcat cctccggcac tcggttttct
     1621 tctaataatt ccgataacat acgaagcgat gccaggggcg tccgaagctc atgggatacg
     1681 gtcgatacga atccggcgcg caaagaactg agttttctgg ccgcccgcat ccgcgcaaac
     1741 aaaaatgccg caagcacgaa cgcaacgacc atcgcaccaa ccccaacccc cgccaataca
     1801 atgcgtgctc gagacgtgcg ccgatcgatc aacagaggat ctgccaagga tacctgcaca
     1861 ccgagggaag gggtgatcca agaaacgctc ttgaaaggag tggtgtcgcg atccatctcg
     1921 gcgggagtct cgatgagatc cacgcgataa tccgcggatc ccgtcgacca accgtgggcc
     1981 atcgcccgac gaacggatgc cgcgtggatg acgaaaccag cgcgcattcc cgtggatatc
     2041 gttttcaaat agccaatcga tgcatccgaa ttccattcga tgatttctgg cgtcgtggcg
     2101 ccatcatgaa gaaccggcct ggggttgggg tccggcaata ccgcacgaac cgcctcatgg
     2161 ttggtcgaat ccgctgataa tgctgtcagg acgcgttttc gggccggtcc cgtgagggta
     2221 ggcaatgcga ggcttgcccg catcgttgcc tcccgttcgg aggggcgcat cgaggaagca
     2281 tggctttcca tccacgaggc cagaccatcg gcgtgctcgg gcgtgaaaga ctccatcgcg
     2341 atgagcggcc agacccaacg ccccgtgacg cttcgatatt cctgacactg atgcatgaag
     2401 tcatcgcgaa gccccccatg ggacggcgac gatcgagatc gcgcgagttc atctgccatg
     2461 gtcccacaac gagggggagc gattcccttg ggctcggaag taatggcttt tcgggcagga
     2521 atgagcagtt ctccctcgga ggagagcacg accgcgtccg cgatcgcggg cgtgatcgat
     2581 cgtaacgcgt tttcgagcaa aaccggatcg gtcggcaagg agctatcgcg aagacggttc
     2641 gagccagcgc gtgtcatggt ttgtgattcc atgaccatgc gtttggccat ggcctcgatg
     2701 gtcacctgga tttcacgacg ggtagcatct tcctggccct gcaaagcgcg caatccgatc
     2761 catcccatgg caagcgcggg cgtgagcgca atgaccgtaa atcgcagcca atcacgcatc
     2821 gtctcgccaa tcgcccgtgt gtcgctcgac atcctgccat tatcgcatgg gccatgtcat
     2881 ggccatgtca caggagaaaa atatgttgaa tatcatagac ttgtattagt tccgggcagt
     2941 catgtcttgg aagggtcggc gacgattccc aggaataaga tgaggttggt ttcgttgtct
     3001 cggacgacga agacgaaggg atggtcggct ttgaacgcgc gcggacgcaa aatggcggat
     3061 gcggttcgag ccatggttac ggcagttgcg gcggctgctt ctgttccttt ttcgtccgtg
     3121 cggatgaatc ctttgtggaa aacctcggag atatagagtc ggtcctgagg gttcggagga
     3181 ttggcgattt tcgaaaaatt cgcgttgcgg cgatcgaaag cggtgcgcat acccagttcg
     3241 cgcaacggcg tactcagtcg catcggagcg ctgggttcga gtttgaagga ggggatcgat
     3301 acccatacgg attcatgcga aagcccgttc gtgagttcgc gccaacgcgt ttcgtccatc
     3361 gatgatagcg cggcatcgag cccgtcgacc gcgtcgggaa gaacgatgat catggatagg
     3421 tcgttgcctt cgtagggaag ttcgagagcg gtaacaccgt ctttggaggc gattcggtac
     3481 gaacccgtgc ggctcatggt gggcacggag atggtattcg aaggcgaaag atgaaacgga
     3541 ttgggctggg tgcgttgttt gtcgaagggc gcggcccaat cgccgagaaa gtagacggcg
     3601 ttgaccaaca caagacgtgt ctccgaatcg acgccgcgat tgggaattag gttttgaatt
     3661 cggttttccg tttgttgctc cacccattcg ttgatgagcc gtcgcgtggg ttcgtgcgcc
     3721 gttttgaagt tgacctgttg catgggagcg ccaaaagcac gggcggtttg cgaaaggaag
     3781 gaaggctcga agggatacgt tttctccccg aatagctggt tggccacacg aaaggtaatg
     3841 ggccgagatc ggtcggtcag cgctgcaatc agcttcccac ttgcctccat cacttcggaa
     3901 ctggaggact caaaatgcag gacctgttgc atttccttgg cggtctgccc gtccgcgcct
     3961 ccccacgtca tggataacgc aaacgaaatg ctcgcgggag aaaacacgag gttgcccggc
     4021 tgcgcacgag aaagcttggg gaataggtcg aacgccagac gattgatcga tgtcgcgagt
     4081 ttggtcatcg tatcaccggt ggaatccgcg gttggggaat cgggcgcaac cggttcggtg
     4141 gggggtaaga ccaccgacga tggtgctggc tccggttgtg gggaagagcg ggggacggag
     4201 gactgtgggg gttggggacc gcaagcggta acgatcgtgg caacgatcaa gggaagaacg
     4261 cgtttcgata agcgatacat gatggtgctc ctcaaaggat tcagaggctt cggcccgagc
     4321 atgataggcc cgagggatgt aggaaagaaa cgaatgaatg cgggtatcct tacagggatg
     4381 cgcgtggtcg atcttcccaa acgcggttgt ggctgtggcg agagcctgcg tcacgggatt
     4441 ggatggctaa gcgtggcaac cataaaaggc gcacgacttg agagagccat gaccacggct
     4501 gttgcgacag gcttgcgctt cggcagttcg ggttcgtgcg taacgcggga aaatgcgccg
     4561 taacaacatc catgctggtg ataggtccta cacatttttc catttgatgc atgacgccgc
     4621 ttcgcttccc gtgagataga tgccagcgga ggtgctgctt gaaaagcgat ctgttccgtc
     4681 gaaagggctt ggaaaagata ttggcggtca cgaacgacga gaaccattcg ctcaagcggt
     4741 cgttgggtgc gttgcacgtg accctgttgg gaatgggggc gatcattgga gcagggattt
     4801 ttgccacgat cggcacggca gcggcgggag atgcagcacg gccgggggct ggcccctcgc
     4861 tcatgctctc gttcgtgatc acgggcatcg tcgccgcgct taccgctgtt tgctatgcgg
     4921 agctggcatc cctcgtgccc attgctggat cggcctacac ctattcgtat gccacgttgg
     4981 gagaactcgt ggcgtgggtc atcggctggg acctg
//
LOCUS       DAOSOR010000447         5011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_16885_length_5011_cov_4.377119, whole genome shotgun sequence.
ACCESSION   DAOSOR010000447 DAOSOR010000000
VERSION     DAOSOR010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16885_length_5011_cov_4.377119"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PK710_19290"
     CDS             <1..354
                     /locus_tag="PK710_19290"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOT11932.1"
                     /translation="VTEGHTVMHLPFREQFVGDSARPALHGGVLSALADACGGTAVWS
                     TVGPEDRVSTIDLRIDYLRPAPLEELVATADVLRVGNRVGVATIRIAACSEPEQVLVE
                     GKGVYAIRRGEDRGG"
     gene            347..1243
                     /locus_tag="PK710_19295"
     CDS             347..1243
                     /locus_tag="PK710_19295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11933.1"
                     /translation="MVEARGGYRWVWLGWGIWLGCSPAPAPTAPPLPPPISPPSSSLP
                     VVHNIPKSLDISEDEPPPDPRPPPLEHPDPFLFLQVQGFGDAVVSLALGARERRPILV
                     AAHGNYDTPESQCWIWREIVGDDGFVLCPRGVVRPDSPSRNDLRYEYVSNQQLEKEIE
                     AGVEALDLAYPDHVDAEAIVFTGFSQGAIMGASIMARRPERYPRAVLIEGGQRVWYPA
                     HAKAFSEGGGQRILWACGQWECHRQAVEAAKVLEKFQIASRVVFSKGEGHTYGGGVAD
                     EIRAAFSWVVQGDERWARETRH"
     gene            complement(1303..1794)
                     /locus_tag="PK710_19300"
     CDS             complement(1303..1794)
                     /locus_tag="PK710_19300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11934.1"
                     /translation="MKCPYVFDQEFLDDLPYEPRVLLVDRIESFDEQARSFVCRMPTD
                     VPMPFTDAQRVHPVKHPHHVSGAAMVHVTGMLGFVHVFYMLGLRHAQGWTGYGTHLHR
                     AVFRKLVPPGKPMLCSCKELRTRFMGDRIFITYEFEYRHEGDIAYESKQSAMWLKTDP
                     TTS"
     gene            1974..3161
                     /locus_tag="PK710_19305"
     CDS             1974..3161
                     /locus_tag="PK710_19305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOT11935.1"
                     /translation="MTTSEPVTPSTRLRFPISLQRSDASALKEAAETIQADKLLMSVL
                     EAIPSFAMVLDENRQIVAANRRLLDAVGAQTLQEIMGQRPGEAVHCRHACRSPEGCGS
                     TAACVFCGAVQAIVISQQTQTTQVQECRIPLMNSKEGAIDAEVSASPIFVGGKCFTLA
                     TMRDISSEKRRKVLERVFFHDVLNTAGGMQGLVAMLRDKPSMEAGIEYVELLQDLCER
                     LVEAIGQQRELLAAETGELVTRPVFVSVRGLLESIQKVYERHEIAGGRKVVLGQAPDI
                     DIVTDANLARRVVENMVKNALEASARGATVTLDAQDQGEQVALRVHNPSVIPDAIKHQ
                     IFQRSFSTKGSDRGIGTYSMRLLGEGYLGGKVSFTSSEPSGTTFVFRIPKTWAGRPSD
                     ESW"
     gene            complement(3176..3754)
                     /locus_tag="PK710_19310"
     CDS             complement(3176..3754)
                     /locus_tag="PK710_19310"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HOT11936.1"
                     /translation="MTIPPARQEPSEQQAETSKGPRWTPPTVHVLTMFSVVRATEALL
                     WPDPFAETDPAIWGQHYKEAWTRPPIFEPSRPAFSWDGDRWEINVIGHGLMGSEAYLR
                     ARQCKFGWLGALAFTAGSTVVWEYGFEANGVRPSAQDLVYTPISGLALGEARFVLWKS
                     ASQISHPTLRSVLRGLVDPFGELSRGTSIFDC"
     gene            4108..>5011
                     /locus_tag="PK710_19315"
     CDS             4108..>5011
                     /locus_tag="PK710_19315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11937.1"
                     /translation="MNPFIIKRLFLAPLVLACAGPGTSDGHDVASNPFPDSTTEKRPW
                     NQAIIQEDRTSLETQEASSDRVASLILLASPSGDLSVLDPADGSLLGTYEANAVDIAA
                     WAEAPHAAIIAEGWEDGSGSIRQVRFDSAGGLHKMGEYALSLDDLRLIHGPEPMLGLG
                     IREGTTIFCGEEGRTLFGTSSFVTFRGNGFVDTWLLEHADSMPTALVLRYEQGFRELE
                     RFQIPTPDYPCPSRFVREAPTPLIIGVMDHDVWLQHPSGEMVMHRSDRESSEGCVQDA
                     LWIDSENAIAVLTGPDARFHLLSVN"
BASE COUNT         1037 a         1322 c         1581 g         1071 t
ORIGIN      
        1 gttaccgaag ggcatacggt gatgcattta ccttttcggg agcagttcgt gggcgattcc
       61 gcgcgtcctg ccttgcatgg tggtgtgcta tccgcgcttg ccgatgcctg tggcggcacg
      121 gccgtatggt ctacggtagg tcccgaggat cgcgtatcga cgatcgattt gcgcatcgac
      181 tacctgcggc ccgcgccttt ggaagagttg gtagccacgg cggatgtgtt gcgcgtgggc
      241 aatcgtgttg gggtggccac gattcggatc gcagcttgtt ccgagcccga acaggttttg
      301 gtcgagggca aaggcgtgta tgccattcgt cgaggagaag accggggtgg ttgaagcgcg
      361 tggtgggtat cgatgggtat ggttggggtg gggaatatgg ttgggttgct cacctgctcc
      421 agcgcccacg gctccgcctt tgccaccgcc catatcacca ccttcgtcat cccttccggt
      481 tgtacataac ataccaaaat cattagatat ttcagaagac gaaccacctc ccgatcctcg
      541 gccgccgccg ctcgagcatc cggacccttt tctctttctt caagtacaag gctttgggga
      601 tgcggtggta tcgctcgccc tgggcgcgcg cgagcgtcgt cccatccttg tggccgcgca
      661 tggcaactac gacacgcctg agtcccaatg ctggatatgg cgtgagatcg tgggagacga
      721 tgggttcgtg ttgtgtcccc gaggcgtggt tcgccccgat tctccgtcgc gaaatgatct
      781 tcgctacgaa tatgtgtcga accaacagtt ggaaaaagag atagaagcgg gggtggaagc
      841 gctcgatctg gcgtatccgg atcatgtcga tgccgaagcg atcgtattca cggggttttc
      901 ccaaggggcg atcatgggtg catcgatcat ggcacgccgg ccggagcgat atccgagggc
      961 ggtattgatc gaaggagggc agcgcgtctg gtatccggct cacgcaaagg cgttttcgga
     1021 aggcggagga caacgtattt tgtgggcttg cggccagtgg gagtgtcatc ggcaggccgt
     1081 cgaggcagca aaagtgctgg aaaaatttca gattgcttct cgcgtggtgt tttcgaaagg
     1141 cgagggtcat acctacgggg gtggtgtggc cgatgaaatc cgtgctgctt tttcgtgggt
     1201 ggtgcaaggc gatgagcgtt gggcgcggga gacgcggcac tgatgatcac ggagagatgg
     1261 caacacgcgg caaaggggaa gtcagcagtg gctcatgcga tgtcatgacg tcgtggggtc
     1321 ggtcttgagc cacatggcgc tctgcttact ttcgtaggcg atatctcctt cgtgacggta
     1381 ttcgaactcg taggtgatga agatgcggtc gcccatgaag cgtgtccgaa gctctttgca
     1441 gctacacagc atgggttttc cggggggcac caacttacgg aaaaccgctc gatgaaggtg
     1501 agtgccatag ccggtccatc cttgtgcgtg acgaagcccg agcatgtaga acacgtgaac
     1561 gaagccgagc ataccggtca catgcaccat ggccgccccg gatacatgat gagggtgctt
     1621 gacggggtga actcgctgcg cgtcggtgaa cggcatggga acgtcggtcg gcatgcggca
     1681 tacgaaggac cgagcctgtt catcgaagct ttcgatgcgg tcgacgagca agacgcgggg
     1741 ttcataagga aggtcgtcga gaaactcctg atcgaaaaca taagggcatt tcatggggaa
     1801 caaggcctcg ggcctctcct aaacccatcg ctaggtggag gacaaggccg tagattcagg
     1861 ttttcgggct ttcggtcttt gcatcgcaaa aagctgaaac ggttgccatg tcgccaacga
     1921 agcgttaatg atgctgattc ctccccatgc gtccccctcg ccgaggaagt cccgtgacca
     1981 cgtcagaacc cgtgacgccg agcacccgct tacgttttcc catttctttg cagcgatcgg
     2041 atgcgtccgc gctgaaggaa gcggcagaaa cgattcaggc cgacaagttg ttgatgagtg
     2101 ttctcgaggc cattcccagc ttcgcgatgg tgctcgacga aaaccgccaa attgtcgcgg
     2161 ccaatcgtcg cttgctcgat gcggtggggg ctcaaactct tcaggagatc atggggcaac
     2221 gaccggggga agccgttcat tgtcgtcatg cctgccgctc gccggaagga tgcggatcca
     2281 cggccgcgtg tgtattttgc ggcgcggttc aggcgattgt catcagtcaa caaacccaga
     2341 cgacgcaggt tcaggagtgt cgtatcccct tgatgaacag caaagaaggt gcgatcgacg
     2401 ccgaggtttc ggcgtctccg atcttcgtgg ggggcaagtg cttcaccctc gccaccatgc
     2461 gcgatatcag ctcggaaaaa cgtcggaagg tgctcgagcg cgtttttttc cacgatgtgc
     2521 tcaacaccgc ggggggcatg caggggctgg tggcgatgct gcgagacaag ccatcgatgg
     2581 aagcgggcat tgagtatgta gagctattgc aagacctttg cgaacgtctg gtggaagcga
     2641 ttggacagca gcgcgagttg ctcgccgcgg aaacggggga gcttgtaaca cgtcctgttt
     2701 tcgtatccgt gcggggtctg ctcgaatcca tccaaaaagt gtacgaacgc catgaaattg
     2761 caggaggacg aaaggttgtc ctgggccaag ctcctgacat cgatatcgtg accgacgcga
     2821 acctggctcg ccgagttgtg gaaaatatgg tcaaaaacgc cttggaagcc agtgcccggg
     2881 gcgccacggt gacgctcgat gcgcaggacc agggcgaaca ggtcgctttg cgagtccaca
     2941 atccgagtgt cattcccgat gccatcaagc atcagatttt tcaacgttcc ttcagcacca
     3001 aaggaagcga tcgaggcatt ggcacctaca gcatgcgact gcttggcgaa gggtatcttg
     3061 gtgggaaagt gagtttcacg tccagcgagc cgtcgggcac gacgtttgtc tttcggattc
     3121 ccaaaacctg ggcgggtcgt ccttccgacg aatcgtggtg atatccgcgg aggaatcagc
     3181 agtcgaaaat actagttccg cgggatagtt caccgaatgg atccaccaga ccacgaagca
     3241 cggagcgaag ggttgggtgt gagatctggg acgcggattt ccaaagaacg aatcgagctt
     3301 ctcccagggc caatcctgaa ataggcgtat acacgaggtc ctgggcggag gggcgcacgc
     3361 cattggcttc gaaaccatat tcccaaacga cggtgctgcc cgcggtgaag gcaagagcgc
     3421 cgagccatcc gaacttgcat tgacgagcgc gcagatacgc ttcgcttccc atcaatccat
     3481 gcccgatcac gttgatttcc catcgatccc catcccatga aaacgcggga cggctcggct
     3541 cgaaaatggg aggacgggtc cacgcttcct tgtaatgctg tccccagatg gcggggtcgg
     3601 tttcggcgaa aggatcgggc cacagcaggg cctcggtggc tctaacgacc gaaaacatcg
     3661 tcagcacatg aacggtggga ggtgtccacc tagggccctt cgatgtttct gcttgttgct
     3721 ccgaaggctc ttgccgcgct ggaggtattg tcacgggctg cgcgagaaag cctgaaacca
     3781 acaatgccaa agggcccata ggtgtcgttg taccgtactt gcacgacggc tggatccgat
     3841 ttgtgaaggg tcggggggtg acgggttggt ggtgatacgc tcgagccaac ggctcgtcat
     3901 cggggcatcg tattgttcca tcggcgcaac aggtggcggc cgccgatttg gggtggcgaa
     3961 ccgccaccag caaaaatctc cgggattttt cgcgaaactc gtcgatttcg cgctgcgcga
     4021 gggtggcgcg ccgccacctt gttcctcttg attcatcgga aaaacagcgt cgattcgatt
     4081 ggcacgcctt tcgctgagtc cgagggtatg aacccattca tcattaagcg attgttcttg
     4141 gctcccctgg tgctggcttg tgcgggaccg gggacaagcg atggtcacga cgtggcatcc
     4201 aatccatttc cggatagtac gaccgaaaag agaccttgga atcaagcgat catccaggag
     4261 gatcggacca gcctcgagac ccaagaagcg tcatcggatc gagtggcaag cctcatcctg
     4321 ctcgcttcac catcgggcga cctaagcgtg ctcgatcccg cggatggttc gctgctcgga
     4381 acctatgaag ccaatgcggt agacatcgcc gcctgggccg aggctcccca cgcggccatc
     4441 atcgccgaag ggtgggagga tgggagcggg agcatccgtc aggtgcggtt cgactccgcg
     4501 ggagggctgc ataagatggg tgagtatgct ctatcgctcg atgacctgcg gctcatccac
     4561 ggaccggaac ccatgctagg ccttggtatc cgagaaggga caacgatttt ttgcggggag
     4621 gaaggacgaa ccctattcgg aacgagttcg ttcgtgactt ttcgggggaa cgggtttgtc
     4681 gatacgtggc tcttggagca tgccgactcg atgccgacag cgctggtatt gcggtatgag
     4741 cagggttttc gcgaactcga gcggtttcag attcccaccc cggattaccc ttgtccatcg
     4801 cgattcgttc gggaggctcc cacacccttg atcatcggtg tcatggatca tgatgtatgg
     4861 ctacagcatc cttcggggga aatggtgatg catagaagcg accgggaaag ctcggaaggc
     4921 tgcgtgcaag atgcgttatg gatcgattcc gaaaacgcca tcgccgtact gacgggtcca
     4981 gacgctcgct ttcatttact atccgtaaac g
//
LOCUS       DAOSOR010000448         5009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_16899_length_5009_cov_3.953371, whole genome shotgun sequence.
ACCESSION   DAOSOR010000448 DAOSOR010000000
VERSION     DAOSOR010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16899_length_5009_cov_3.953371"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PK710_19320"
     CDS             <1..208
                     /locus_tag="PK710_19320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11938.1"
                     /translation="GEQSQIAPAATFHRNMGMLSMGMMWPTRETPRVPQREPSRVDDA
                     DRDMRLSGPQADGMRSRGSRPRND"
     gene            complement(634..2187)
                     /locus_tag="PK710_19325"
     CDS             complement(634..2187)
                     /locus_tag="PK710_19325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11939.1"
                     /translation="MKSSMNKWPDIGRGWASKTGAKLAVALLVASGAARAETYRVGAG
                     EKYATLQEVANVLSPGDVVEVKGGASYGAVRFERHGSVDKPIVIRGISVGDRRPIIQG
                     GRDTIEAVGDHYVFENLEITGGSFRCFFHRAHDITLRHVVVRDCPAHGILGADEGSGS
                     MLLESSEIFRCGEGDKRHPIYMATDERAHPGSVFRMQFCYVHDGKGGNNVKSRAERNE
                     IYFNWIEGALYHELELIGPDGADPALAREDSEVIGNVFRKTNDAFSVRVGGDGTGETN
                     GRYRFVHNTFLLEANNRAVFRLFDGIESIEMHNNAIYAKGGGPITVVRTSDAKWSTGR
                     ELISGANNWIPKGSTSVPSGWQGTLVGTDPMFENLGELDLRPSAQSPLRDAAAPMQAL
                     AAPPSYAFPRPRAELGFQPPRRAIGPRKLVRASDGKPDIGAYEGGFEFAEAKTVETIP
                     IDQPSRGADAPALGPVPGSGIGPSRPTAAGGRCGCGLPGDVKEKAPWSLVGIGLIAIR
                     RLRNRAKGC"
     gene            complement(2135..3286)
                     /locus_tag="PK710_19330"
     CDS             complement(2135..3286)
                     /locus_tag="PK710_19330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT11940.1"
                     /translation="MNDTINVCFVVNNFNVGGLEKVAIDLVNHLDRSRFKPFVVCLDG
                     RGLLFDRLEIDESASLILDKSGSKRTPVGTFDPRMLWRMRSFFVRHGISIVHAHNLGP
                     LIYGGLSARLGFRRPTVVYSEHNQIYSASASTRRKFVFYIRLADHVVAVSHDLKRTLT
                     RRVRPSCPVRVLHNGIDGSKFALADGSEVRSELGVGGDEILIGSGVVLSKQKGIPYLL
                     QAAKMVVEKEPRARFVLAGQGPLRTELEQMAERLHLGDRFVFLGYRADMPRIISALDI
                     YVLSSLWEGLPLALLEALAMGKPIVATRVGGNPEVVQDGVNGFIVEPRDVNGLARALL
                     RTCQDDAFRNSVREVNRARFENEFSLQAMVSAHEKLYEQVAGHRSWLGK"
     gene            complement(3314..4378)
                     /locus_tag="PK710_19335"
     CDS             complement(3314..4378)
                     /locus_tag="PK710_19335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT11941.1"
                     /translation="MTYQAFAARAEHRESLERLWTQAMSDRRIALVRDRRWSWLYERN
                     PAGPAYTYVVQHVESGQIVGSASVYPRDIVVGGQSYKAGVLADFVTHKAHRVAGPAVM
                     VQRAIMEEHRQRGLDFLFGYPNKGAAPIFPRLRFKTVCESSLWVKPLRTARKLSLFLH
                     PALAIGLAPFANAVLATNDLRLMVSRRPMGAAVMGHVPDQAFDDLWERCRGQVPIFGV
                     RTSAFLRWRYIEHTTERYKVFTVQRRDGGRVYGYVIFSIRENRVYVADMLAEGGVRGA
                     ESLLLAFCRAMRRRGHESVCINYAGDERFFASLRALQFLRRVGARKLIAFVAQDQPEA
                     LRNLVYDHSGWFMHDGELDI"
     gene            4487..>5009
                     /locus_tag="PK710_19340"
     CDS             4487..>5009
                     /locus_tag="PK710_19340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11942.1"
                     /translation="MKRSVISLFCVAACWLVACGSDSDDSAHHNTSGTGGSANPTDAA
                     TSDSSNDATNDATSDTSEDVTMDTPDEEFEPDVAESSVDVAPDSYEDVEQDSPQDVTQ
                     DVAQDITQDIAQDITQDVAQDSPQEVALDVAFDSSGRPPGQCKSNSDCSGPAAECSMS
                     APGGICMGCGSATD"
BASE COUNT         1154 a         1655 c         1221 g          979 t
ORIGIN      
        1 cggcgaacaa tcccaaatcg cccccgccgc taccttccac cgcaacatgg gcatgctgtc
       61 catgggcatg atgtggccta ccagggaaac cccgcgcgtc ccacaacgag aaccatcacg
      121 cgtcgatgac gctgaccgcg atatgcgact cagcggcccc caagccgatg gcatgcgctc
      181 cagaggctct cgccctcgta acgattgacc ctcgccgccc acccaccgtc acacctccat
      241 cacctatccc ttctgctttc gtaactatac atatccgtat agttatggtg gccctgtttg
      301 cgacgcaacc ttccacctca cccccgcccc actttccgcc ttttctgcct ccatccctcc
      361 ttatcaccac cgcttcgctt cccccttctg ccatcctccg atgtccccat tcttaatctt
      421 attccataat attcgtatgt tataacatcg atggcaggat ctcatggggc actcccccac
      481 aggccccggc ttcagccagg ccactggacc tccgaagcac acgccccctc cgacgcatga
      541 ctctgtacca accctgtgga ggttcgagcg cttcgaaggt gaaataatac ctattcggat
      601 agcacgtcgt tcatgccaca acgcatcctt cgatcagcat cctttggctc ggttgcgcaa
      661 tcttcgaatg gcaatgagcc cgatccccac caatgaccac ggagcctttt ctttcacgtc
      721 accgggcaac ccacaaccgc atcgacctcc tgccgcagtc ggacgcgatg gtccgatgcc
      781 agaccccggg acgggaccga gggctggcgc atcagcgcca cgggagggct ggtcgatcgg
      841 aatcgtctct accgttttcg cttcggcaaa ctcgaatcct ccttcatagg cgccgatatc
      901 cggcttgcca tccgaggctc gcacgagctt tcgcggccca atggctcgac ggggcggttg
      961 gaatccgagt tccgctcgtg gtctgggaaa ggcataggac ggtggagccg ccaacgcttg
     1021 catgggtgca gccgcgtccc gaagcggact ttgcgccgac gggcgcaagt ccagctcccc
     1081 caaattttca aacatggggt cggttcccac caacgtgccc tgccatcccg acggaaccga
     1141 tgtcgaaccc ttgggtatcc aattgttggc gccactgatc agttcccgac ccgtggacca
     1201 tttcgcatcc gacgtcctca ccacggtgat gggtcctccc cctttggcat agatcgcgtt
     1261 gttgtgcatt tcgatgctct caatgccatc aaagagccga aatacggcgc gattgttggc
     1321 ctcgagaaga aacgtgttgt gaacgaaacg atagcgacca ttcgtctctc ccgttccatc
     1381 gccaccgaca cgaaccgaaa acgcatcatt cgtttttcga aatacgttgc caatcacctc
     1441 cgaatcctct cgcgcgagag caggatcggc accatcggga ccaatcaact cgagttcgtg
     1501 ataaagcgcc ccctcgatcc agttgaaata aatctcattg cgctccgcac gactcttgac
     1561 gttgtttccc cccttgccgt cgtgaacata acaaaactgc atgcgaaaaa cggaaccggg
     1621 gtgagcccgc tcatcggtcg ccatgtaaat cgggtgacgt ttatcccctt cgccacagcg
     1681 gaaaatctcg ctcgattcga gcaacatcga accggaaccc tcatcggccc ccaatatccc
     1741 gtgcgcggga caatccctca caacgacatg acgcaaggta atgtcatgag cacgatggaa
     1801 aaaacaccga aagctccctc ccgtaatctc gaggttttcg aagacataat gatcgcccac
     1861 cgcctcgatc gtatctcgcc ccccttgaat gatcggtcgt cgatctccta cggaaatacc
     1921 gcgaatgacg atcggcttgt ccacggaacc atgtcgttcg aagcggacgg caccatagct
     1981 cgcgccccct ttgacctcca ccacgtctcc cggggataga acgttcgcca cctcttgcaa
     2041 ggtggcatac ttctcccccg ctcccacgcg ataggtctcc gctcgcgccg cgcccgaggc
     2101 aacgagcagc gctaccgcca gcttcgcgcc cgttttactt gcccaaccac gaccgatgtc
     2161 cggccacttg ttcatagagc ttttcatgtg cgctcaccat cgcttgcaaa ctgaattcgt
     2221 tttcgaatcg ggcacgattg acctcgcgaa cgctgttgcg aaacgcatcg tcctgacaag
     2281 tccgcaacaa cgctcgcgcc aaaccgttca cgtcccgcgg ctcgacgatg aacccattca
     2341 caccatcttg aacgacctcg ggattgccac ccacgcgagt cgccacgatc ggttttccca
     2401 tcgcaagcgc ctccaacaat gcaagcggca agccttccca taacgatgac aagacgtaaa
     2461 tgtcgagcgc ggagatgatc cgcggcatat ccgcgcgata tccaagaaag acgaaacgat
     2521 cgccaaggtg caggcgttcg gccatctgtt ccaattccgt acgcaagggc ccctgtccgg
     2581 caagcacgaa ccgcgcccgc ggctcttttt ccaccaccat tttcgccgct tgaagcaagt
     2641 acgggattcc cttctgcttg gacaaaacca ccccagaccc aatcaggatt tcatccccgc
     2701 caaccccaag ctcggaccga acctccgaac catccgcaag cgcaaacttc gaaccatcga
     2761 tgccgttgtg caacacacgc acaggacacg aaggacgaac acggcgagtc aacgttcgct
     2821 tcaagtcgtg tgagacggcc accacatggt cggcaagacg tatataaaac acgaacttac
     2881 gacgtgtcga tgccgaggca ctgtagatct gattgtgctc ggaatacacg accgtaggac
     2941 gacgaaaccc caaacgagcc gacagaccac cgtagatcaa aggtcccaag ttgtgcgcat
     3001 ggacgatgga tatgccatga cggacaaaga atgaacgcat tcgccagagc atgcgtggat
     3061 cgaaagtccc cacgggcgtg cgcttcgagc cggatttatc gagaatcagc gatgcgcttt
     3121 catcgatctc gagacgatca aacagcagac cccgtccatc cagacaaacc acgaaaggct
     3181 tgaaacgact tcgatcgagg tgattgacaa gatcgatggc gactttctcg agcccgccca
     3241 cgttgaagtt gttaacgacg aaacaaacgt tgatcgtatc gttcatggcg caccaaactt
     3301 cggttcccgg acgtcagatg tcgagttctc cgtcgtgcat gaaccatccg ctgtggtcgt
     3361 aaacgagatt tcgcaatgcc tcgggctggt cttgcgccac gaaggcaatg agtttgcggg
     3421 caccgacgcg ccgaaggaac tgcagcgctc gtaacgaagc aaaaaatctc tcatccccag
     3481 cataattgat gcataccgat tcatgccccc gccgccgcat cgcgcgacaa aacgccagga
     3541 gcaacgattc cgctccccgc accccacctt ccgcaagcat gtccgccacg tacacccggt
     3601 tttctcgaat cgaaaaaatg acatagccat acacgcgccc cccatctcga cgctgcaccg
     3661 tgaacacctt gtatcgctcg gtggtatgtt cgatgtatcg ccatcgcaaa aatgccgacg
     3721 ttcgcacgcc aaatatcgga acctgcccgc gacaccgctc ccatagatca tcaaaagcct
     3781 gatccggaac atgccccatc accgccgccc ccatgggtcg tcgcgatacc atgagacgca
     3841 agtcgttggt cgctagcacc gcgttggcga agggagccaa accaatcgcc agggcgggat
     3901 gcaaaaaaag tgataatttt cgagccgttc gcagcggctt gacccatagc gaggactcac
     3961 aaacggtttt gaagcgaagg cgcgggaaaa tcggcgctgc ccccttgttc ggataaccaa
     4021 aaagaaaatc gagaccacgt tggcgatgtt cctccatgat ggcacgctga accatgaccg
     4081 cgggaccggc gactcgatgc gccttatgcg tcacgaaatc cgccaacacc ccggccttat
     4141 acgattgccc ccccaccacg atgtcacggg gatagaccga cgcggacccc acgatttgcc
     4201 ctgattccac gtgctgcacc acgtaggtat acgccggccc cgccggattg cgctcgtaca
     4261 accacgacca tcgtcgatcg cgcaccaacg cgattcgccg atcgctcatg gcctgggtcc
     4321 aaagacgctc caacgattcc cggtgctcag cccgcgctgc aaatgcctga taggtcatga
     4381 ttccttcctg acgccgtagc actgccgtgc ttcagggaac aaggctcgac caggaagaac
     4441 gaattcagac aggacgaaac cacccgctga tgctacaccg aatgccatga aaaggtcggt
     4501 tatctccttg ttttgtgtcg ccgcctgctg gctcgtcgct tgcggctccg atagcgacga
     4561 ctccgctcac cacaacacct cgggtacggg tggttctgcc aacccaacgg atgccgctac
     4621 gtcggattca tccaacgatg ctaccaacga cgcaacttcc gatacctccg aggatgtaac
     4681 gatggacacg ccggacgagg aatttgaacc cgacgttgcc gaaagctctg tcgatgtcgc
     4741 tccagattct tacgaggatg tcgagcagga tagcccgcaa gatgtcaccc aagatgttgc
     4801 gcaggatatc acccaagata ttgcgcaaga tatcacccaa gatgttgcgc aggattcacc
     4861 ccaagaagtg gcgctcgatg tggcttttga ttcctccgga cgaccgcctg ggcaatgcaa
     4921 gtccaacagc gattgctcag gaccagcggc agagtgttcc atgagcgctc ccggcgggat
     4981 ctgcatgggg tgcggctcgg ctacggatt
//
LOCUS       DAOSOR010000449         4998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_16940_length_4998_cov_3.312159, whole genome shotgun sequence.
ACCESSION   DAOSOR010000449 DAOSOR010000000
VERSION     DAOSOR010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16940_length_4998_cov_3.312159"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1424)
                     /locus_tag="PK710_19345"
     CDS             complement(<1..1424)
                     /locus_tag="PK710_19345"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOT11943.1"
                     /translation="MIRKRPLLVVGIASFQLACGAHESGLDPVATQPAKDAAVVDVQE
                     DVQESMDAKEAGQTDVAQEEDTPKKQDRGDPEQFPSSCLDTCQQACASLHDCGGDSSV
                     YPLDELECVERCAMAMSSPYDLWDDVSGHFRCCASQDACFDVATCGGWLAHPAPGSSC
                     EKLCDCLGQYMTVPSWPADVQPPQGYRFASNAMAMEGSVPHAISAYPGVEIIGRGRVT
                     TLRFSFPIQEHRLRALAGGARSLPTFYDGAGRLAVAMGDIFIELGDMSQVGVLRARAK
                     SAHFSPPEAVSFGRNLFRIASADPWKSLRALPHLRSIPKARVELDMLRYYKTHHIPND
                     PLFLDQWHLRNTGQHESISGVDGRVTEAWDTTMGTPEVVIAINDDGVDVTHPDLAASC
                     LEPLNFPSDWQEQMTDPLSGFGAHGTSVAGVAASIADNGQGGAGVCPRCRIMPHLVGE
                     KVGPTGLSMTDQGIANGFVQMVDEG"
     gene            complement(1546..3255)
                     /locus_tag="PK710_19350"
     CDS             complement(1546..3255)
                     /locus_tag="PK710_19350"
                     /inference="COORDINATES: protein motif:HMM:NF024840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4114 domain-containing protein"
                     /protein_id="HOT11944.1"
                     /translation="MKTWTPFLFTLLISSSAFAVTQPNGKTIPVQSVYCDGGKTAGLA
                     AVFACICEQPGVCNIGDPCPGNQDPNSCDKGQKGTCETTLWHNWNEDPCIPKNLSGLD
                     PVADADIQPETFRPVCPMQFRIESRGTAMFRDIFGWYNVTGSKPSLDDLHVMLDCNTA
                     AGKTVELDLQSEPAYLGGEVGFFLATPQDPNHGPTCANGDCCATLDRIKKGEGFVYYS
                     EPKYNPDTAGQIHLLIYDSRISDYKFYFAWEDIYGGDNDDFTDLVTSVSGIQCSGGGL
                     PCDTGKPGICSYGITECDSSGAIVCKQVFAPSAETCDAIDNDCDGRADNDATCSGDRI
                     CHQGRCVKSCSSGEFPCQGAYQQCDYTSGLCVPSDCVGVSCDADKVCRAGKCVAPCEG
                     VVCPWGQSCIGDVCLDLCEGVSCAPGQVCRGGACFDGCGQCSGATCEAPLRCDTASGE
                     CFDPSCASPCPAGQHCESGDCVSNCKDAVCPGGASCVDGRCQGTDASGSTDGGFDPGD
                     TGGNPLQDGGVEQEGGDNSGSSLYSKGESSGCGCRVAGSVSSTLGLMNLLWFGLWLVR
                     RRR"
     gene            3450..>4998
                     /locus_tag="PK710_19355"
     CDS             3450..>4998
                     /locus_tag="PK710_19355"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOT11945.1"
                     /translation="MVARWIALIVSISAWAAGCADPSVPSESPATPPLVSSQPLASSP
                     VTSASARVHPSPPAEPMPSPVPRIGSLTQDAYIRARPDHKAPEIGTLDLGSTVALRQP
                     SPVGTAGCRGGWYAVEPYGFLCHDGTTTLEPDTHPLMQAKRAHHLSDQACPYSWGESR
                     FAPLYRSIPDAREQRRWEPKLDKHLAALEKLREARRAGASEDEVPTPSPLRGVDIFAA
                     SSPAPAFLANGNPSPWAAVHTPTDLRPRYDTVPARSSIAWTDEFFAEGRSWLLTPQLL
                     LVPKDKVSIQKPSSYSGVRLSSDGIHLPLAFIRGQEKTKYRWEEQGVAQESASGTTIP
                     AAWSPRAEFKEDADSPKGAFVATAETWKRLEHVQLTGKARWKRGKRYLETNEQGRWIR
                     ESDAVVVRGRPPDGLELREGEKWIDVSIHWGTLVAYEGSKPIFATLISPGLKGYARVQ
                     GKPAKNTSPTGAFRIEWKHLTRTMSPDPEKKTYYLSEVPFTQFFHLPFALHGAYWHDA
                     FGEPKSGG"
BASE COUNT         1149 a         1706 c         1195 g          948 t
ORIGIN      
        1 ccctcatcga ccatctgaac gaaaccattg gcaatccctt gatccgtcat gctcaagccc
       61 gtcggcccca ccttttcgcc aaccagatgc ggcatgatgc ggcacctcgg acataccccc
      121 gccccccctt gcccattgtc cgcaatcgat gcagccactc ccgcgaccga cgttccatgc
      181 gcgccaaatc ccgacaacgg atcggtcatc tgctcttgcc aatccgatgg aaaattcaag
      241 ggttccaaac aggaagcggc aagatccgga tgcgtgacat ccaccccatc atcgttgatc
      301 gcaatcacca cttccggcgt tcccatcgtc gtatcccacg cttccgtcac acgaccatcg
      361 acccccgata tcgattcgtg ttgccccgta ttgcgcaaat gccactggtc caaaaagagc
      421 ggatcattcg gtatatgatg cgttttgtag taccgaagca tgtcgagttc gacccgcgct
      481 ttcggaatcg aacgaaggtg cggcaaagct cgcaagcttt tccacggatc cgcactcgcg
      541 atacgaaata gatttcgccc aaaggaaacc gcttccggcg gggaaaaatg agccgatttc
      601 gcacgggccc gaaggacgcc gacttgcgac atatcgccta attcgatgaa gatatccccc
      661 atcgccactg ccaaccgtcc cgccccatcg taaaatgtcg gcaacgaacg cgcaccaccc
      721 gcaagagcgc gaagacgatg ctcctgaatg ggaaaagaaa aacgaagcgt cgttacccga
      781 cctcgcccga tgatctcgac gccaggataa gcggaaatgg catgcggcac gcttccttcc
      841 atcgccatcg cgttgctcgc aaaacgatac ccttgtggcg gctgcacgtc cgccggccac
      901 gagggaaccg tcatgtattg gcccaggcaa tcacagagct tctcacacga tgaccctggt
      961 gccggatgcg ctaaccatcc accacaggtg gccacgtcga aacacgcatc ttgcgaagca
     1021 caacagcgaa aatgccccga gacatcgtcc cataaatcgt aaggagaact catcgccatc
     1081 gcacaacgct cgacacactc gagttcgtcc aacggataca cgctcgagtc accgccacaa
     1141 tcgtggagcg aagcacacgc ttgctgacat gtgtcgagac aggaggaagg aaattgctcc
     1201 ggatcccctc gatcttgttt tttcggcgta tcctcctctt gggccacatc cgtctgcccc
     1261 gcctctttgg cgtccattga ctcctgcaca tcctcctgca catcgaccac ggccgcatcc
     1321 ttcgctggtt gtgtagccac gggatcgagc cctgattcat gcgctccgca ggccaattgg
     1381 aacgaggcaa tccccacgac gagcaacgga cgcttgcgta tcacgacgac tccttgcaat
     1441 gacgattggt cgaaagacta tggaatcaca cgagccaggc ttcagctagc cgaatgaatc
     1501 cgaaagcaaa gcatggtgtt gatcaaggca aaacggcgcg aggagtcatc gtctgcgacg
     1561 aaccagccag agtccgaacc acagcagatt catcaggccc aacgtggagg acaccgatcc
     1621 cgccacacga cagccacacc ccgaactttc acctttcgaa tacaggctcg aaccgctgtt
     1681 atctccccct tcctgctcca cacccccatc ttgcaacgga ttgccgcccg tgtctcccgg
     1741 atcgaatcca ccatccgtcg agcccgaagc atcggttccc tgacacctgc cgtccacaca
     1801 agacgcacca cccggacaga ccgcatcctt gcaattgctc acacagtccc ccgattcaca
     1861 atgctgtccc gccggacatg gcgaagcgca agaaggatcg aaacactcac cacttgccgt
     1921 atcgcaacga aggggcgctt cacaggtcgc cccgctgcat tgcccacatc catcgaaaca
     1981 agctccgccc cggcatacct ggcccggcgc acagctcacg ccttcacata aatcgagaca
     2041 cacatcccca atacagctct gcccccaagg acaaaccacc ccctcacaag gcgcaacaca
     2101 cttgcctgcc cgacatacct tgtccgcatc gcaactcacc ccgacacaat cggaaggaac
     2161 acaaagtccc gaggtgtaat cacattgctg ataggccccc tgacacggaa attcaccgct
     2221 cgaacagctc ttcacacacc gtccttggtg gcaaattcga tcaccggaac acgtggcgtc
     2281 gttgtctgcg cgtccatcac aatcgttgtc gatggcgtcg caggtctccg cggaaggcgc
     2341 gaatacttgt ttgcatacaa tcgcaccgct cgaatcacat tccgtgatgc cataagaaca
     2401 aatccctggc ttgccggtat cgcagggcaa acctccacca ctgcactgga tcccagacac
     2461 actcgtcacc aggtcggtga agtcgtcgtt gtcccccccg tagatatctt cccaggcgaa
     2521 atagaactta taatcggaaa tgcggctatc gtagatgaga agatggatct ggccggccgt
     2581 gtcgggattg tacttcggct cggaatagta cacgaacccc tccccttttt tgatccgatc
     2641 caaggtggcg cagcaatcgc cattcgcaca agtcggaccg tggttggggt cttgcggcgt
     2701 ggcaaggaaa aatcccacct cgcccccgag gtacgccggc tcactctgca aatcaagctc
     2761 gaccgtcttg cccgcagccg tattgcagtc gagcatcacg tgcaaatcat caaggctcgg
     2821 cttgctcccc gtcacgttat accaaccaaa aatgtcgcga aacatcgcgg ttccacggct
     2881 ctcaatccga aactgcatcg gacagacagg acgaaacgtc tcgggctgaa tgtccgcgtc
     2941 agctaccgga tcgagccccg acagattctt cggaatacaa ggatcctcgt tccaattatg
     3001 ccaaagtgtc gtttcacagg ttcccttctg acccttgtca caactgttcg gatcctggtt
     3061 gcccggacat gggtcgccga tgttgcagac ccccggctgc tcacaaatac acgcgaatac
     3121 cgccgcgagc cccgcggttt ttccaccatc acaatacacg ctctgcaccg gaatggtctt
     3181 tccattcggc tgcgtcaccg cgaaggcaga agaggaaatc aacagggtga acagaaaggg
     3241 cgtccaggtt ttcatcgcaa agaatagcct acgagaggaa ttagtacctc gccactgagt
     3301 tacgtttttt tatctcgagc cagcgacgat ttttccctcc tgcccccccc gccccctcga
     3361 tccaacgccc aattcctgat gccaacggac gatgatgccg gcaacggaac ttggcccttc
     3421 ccctccactt cccccatggt ccggccacga tggttgcacg atggattgcc cttattgtca
     3481 gcatctccgc gtgggccgcg ggatgcgccg acccctccgt tccttcagaa agtcctgcca
     3541 caccgccttt ggtgtcgagc caacctttgg catcctcccc cgtaacctca gcctccgctc
     3601 gtgttcatcc ctccccccct gccgaaccca tgccttcccc cgtcccacga atcggctcgc
     3661 tcacgcaaga tgcttatatt cgcgcccgcc cagaccacaa agcccctgag attggaacgc
     3721 tcgaccttgg ctccacggtc gcgcttcgac agccttcccc ggttggcacg gccggttgtc
     3781 gcggcggatg gtacgcggtc gaaccctacg gctttctttg tcacgatggc acgaccaccc
     3841 tggaaccgga tacccatcct ctgatgcaag ccaaacgagc ccatcacctc agcgatcagg
     3901 cttgtcccta ttcctgggga gaatcacgat tcgccccgct gtatcgaagc atccccgatg
     3961 cccgtgagca gcgacgatgg gaaccgaagc tcgataagca tcttgctgca ctcgaaaaat
     4021 tgcgcgaggc gcggcgcgcg ggagccagcg aagacgaagt gccaacccct tcgccccttc
     4081 gcggagtcga tatctttgcc gcttccagcc cagcccccgc tttccttgcc aatggcaacc
     4141 cttccccctg ggcagccgtg catacgccca cggatcttcg cccccgctac gacaccgtcc
     4201 ccgctcgctc ctccatcgcc tggaccgacg aatttttcgc ggaaggccgt agctggctgc
     4261 tgactcccca gctcctgctc gtccccaaag acaaggtgtc catacaaaaa ccgtcgagtt
     4321 attctggtgt tcggctctcg tcggacggca tccacctgcc tttggccttc attcgcggac
     4381 aagaaaaaac caaataccgt tgggaagaac aaggggtagc gcaagaaagc gcctccggta
     4441 ccaccatccc cgccgcatgg tccccaaggg ccgaattcaa ggaagatgcc gattcaccca
     4501 aaggggcgtt cgtggctacc gccgaaacct ggaagcggct cgaacacgtc caactcaccg
     4561 gaaaagcccg ctggaaacgc ggcaaacgct atctcgaaac caacgaacaa ggacgatgga
     4621 tccgggaaag cgatgccgtc gtcgttcggg gtcgcccccc cgatgggcta gaattgcgcg
     4681 aaggcgaaaa atggatcgat gtatccatcc attggggcac actcgtcgcc tacgaaggct
     4741 cgaagcccat cttcgccacc ttgatttccc cagggctcaa aggatatgcg cgcgtgcagg
     4801 ggaaaccggc gaaaaatacg agccccaccg gcgcgtttcg catcgaatgg aaacacctca
     4861 cacgcaccat gtccccggac ccggagaaaa aaacctacta tctgtccgaa gtccccttca
     4921 cgcagttttt ccacctgccc ttcgccttgc acggcgccta ttggcacgat gccttcggag
     4981 aaccaaaaag cggaggct
//
LOCUS       DAOSOR010000450         4987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_16984_length_4987_cov_4.285888, whole genome shotgun sequence.
ACCESSION   DAOSOR010000450 DAOSOR010000000
VERSION     DAOSOR010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16984_length_4987_cov_4.285888"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3586)
                     /locus_tag="PK710_19360"
     CDS             complement(<1..3586)
                     /locus_tag="PK710_19360"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT11946.1"
                     /translation="MIDLPEHWRFALDTVVGQGASGIVWRAKDKQTNAIAAVKLARDP
                     SSAAALAHEAESLLLVGSPHLPSVLDLGQLPHSETQSPQPQAFLALSWVQGQRLCPQS
                     IPDSQRIAIATAIARDVGAALDDLHQAGMAHGDVSPNNILIDQQATGYTATLIDLGYA
                     VEANTDDLWGLTLRYAPPELAHGQRNAIARDLWGFGLCLAEVLDATIAQSATPATTLW
                     SRDLIAPWGAWLRALTAQQPSARPSAAWISRWITGFRSNARRPARVRTAYLRTRKQTL
                     ALARQTIEVRISESVCPWLEQAVRIVRTAHALRHGSAPNHTTTSLDIQPLDAAGIKRW
                     LVALVGVGATHWSLPSTITQRSERDLAALLERLAHRKDPSLWTLLDVLGVLHDDAIHD
                     VAPKGQDPEDDVPLALALVQRPVSAAAMQVAEQRLWKGSLPEIHRVLLAKAMRSAGGL
                     GTAYAVVDGCSDTSAEVLRADLLRRMGRNEQAIQLATSLIAKSEVTTDHKDEASAILA
                     RAMLDNGHAAQALQVIETCQGAPAAEVRALAQLALGDHPRAIQSMEEGLAVAHNDEER
                     ARLLCVQGMEAHARGASKQAYDSFVQATEYAIGAGSIIEEATYQTGVAAAAVDAGYVE
                     KALDASLRAELLWEHLGRPEKMAFALLARASAYHLVGCTHDARQHAQRALDHARTAGD
                     PRVIAYVRMLMADLQSGEQQQREADLAFQALSAHGIVDDRLRAQARKASMGALSDDQI
                     RKLDLVAESENVGVTAKMEWWTARARAWNGGHRLGRDMEIVSKIVRLIYEPMPIGIKG
                     PGVLAARILAASAADGETTRTLAALQTRLSLDLLEHVPSWLRDSALSVPWVTQVPTGM
                     DQGATVDRVQHLEVLIRAMAERNSLRQVLDRSLDALVLWTGVERGILLLRAPGNRLVI
                     RAARNLERRDLQGDQLELSHSLAQRALVTREPVVAVDASGEMTELHSSVGKLGLRSVL
                     AVPLISRGEVQGVAYLDDRLRKGAFGPQELSWVRLVAGIAAMAIADARSQVLLRRAAR
                     TTARANAILKEQLAQREAELERVEKVLASGQSRPRRGYEAIIGESEPVRRMLALVDRV
                     AATDLPVLVEGETGSGKELVAKAVHDNSHRRSKPFVTENCSAIPEALFESTLFGHLRG
                     SFTGADRRRLGLFEVADGGTLFLDEVGEIPLPLQSKLLRFMQDGEVRRL"
     gene            complement(3669..4649)
                     /locus_tag="PK710_19365"
     CDS             complement(3669..4649)
                     /locus_tag="PK710_19365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11947.1"
                     /translation="MTQPAGETTWIRCALEVPYRYEAVAPITKTIELPNVEATRQLLP
                     ASGEALVALLQAGTPVRLHYQIVGRFVVDTESPRSASIVCPSATHYVRSIALGTYRII
                     VGAEKSVNAVISADTQRDTFGRMPSCATLAPSSPKEGCTVPLAVQLSRIEGHDASAKM
                     RTGDIAKLDHVPRAETMPWIQVNSLSSDQPQAPSQCTAGFELIGDVDQDIVRLTARCG
                     SALGMVAHSEVLTGQQGATDEQARYIVKLQAGQCYRAFGVGGPGVQDLDMGWNSPDGS
                     SIAWDTGPEAWSIAPIHGPLCAPSSGEYELVVSVERGQGAFAVQVWRVAP"
BASE COUNT          988 a         1436 c         1491 g         1072 t
ORIGIN      
        1 ccagtctgcg gacttcaccg tcctgcataa accgaagcag cttgctttgc agcggaagcg
       61 gtatctctcc tacctcatcc aagaacaacg tgcccccatc cgcgacttcg aataggccca
      121 atcgtcgacg atccgcgccg gtgaacgagc cccgtaagtg gccgaatagg gttgattcga
      181 acaacgcttc cgggatggcc gagcagttct ccgtgacgaa cggtttggag cggcgatggc
      241 tattgtcgtg gacggccttt gcgacgagtt cttttccgga tcctgtttct ccttcgacga
      301 gcacgggtaa gtccgtggcg gcgaccctat cgacgagcgc tagcatgcgg cgaaccggtt
      361 cgctttcgcc aatgatcgct tcgtaaccgc gtcgcggcct gctttgtcca cttgccaata
      421 ctttttccac acgctccaat tccgcctctc gttgcgcaag ctgctctttg agtatggcat
      481 tggcgcgtgc ggtcgtccga gcggcgcggc gcaacagcac ttgtgaacgc gcgtcggcaa
      541 tggccatggc ggcaattcca gcaaccagcc gcacccatga cagctcttgg gggccaaagg
      601 ctccttttcg taagcgatcg tccaaatagg ctacgccttg cacctcgcct cgactaatca
      661 gcggaaccgc gagaacgctt cgaagcccga gtttgccgac gctagaatgc aattccgtca
      721 tttctcccga agcatccacc gcgaccacgg gctctcgcgt gacaagcgct ctttgcgcga
      781 gcgaatgcga tagctcgagt tggtcgccct gcagatccct acgttctaga tttcgggcgg
      841 cacgaatgac gagtcgattt cctggcgcgc gaagaagcag gattcccctt tctacacccg
      901 tccatagcac cagggcatcc aacgagcggt cgagaacttg gcgcaacgaa ttccgttctg
      961 ccatggctcg gatcagcact tcaaggtgct ggactcgatc gaccgtggct ccttggtcca
     1021 tgcccgtggg cacctgcgtc acccagggaa cggaaagggc gctgtcgcgg agccaagaag
     1081 ggacgtgctc cagcaaatcc aaagacaatc gagtttgtag cgcagcaagc gtacgtgttg
     1141 tctcaccatc agccgcggaa gccgccaaga ttcgtgcagc caatacgccg ggccctttga
     1201 ttccaatcgg catgggctcg tatatcaaac gaacgatctt cgatacgatt tccatgtcgc
     1261 gtccgagccg gtggccacca ttccacgcgc gggctcgcgc cgtccaccat tccatcttcg
     1321 cggtgacacc aacgttttcc gattcagcga caagatccaa cttccgaatt tgatcgtcgg
     1381 aaagcgctcc catgcttgct tttcgagctt gggcgcgaag cctatcatcc acgattccgt
     1441 gggcacttag ggcttgaaag gccaagtccg cttctcgttg ctgctgctct ccggattgca
     1501 gatcggccat caacattcgc acataagcga tgacgcgtgg gtctccggct gttcgcgcat
     1561 gatcgagggc tcgctgggcg tgttgtcggg catcgtgagt gcatcccacc agatgatatg
     1621 ccgaggctcg tgccaagagc gcgaaagcca ttttttctgg acgacccagg tgttcccaaa
     1681 gcagttccgc gcgaagtgat gcatcgagcg ctttttccac gtagccagcg tcgaccgccg
     1741 cagcggccac accggtttgg taggtggctt cctcgatgat ggagcctgcg ccaatggcgt
     1801 attcggtggc ttgcacgaat gagtcatacg cctgcttgga ggctccacgg gcgtgtgctt
     1861 ccatgccctg cacacacaac agtctggctc gttcctcatc gttgtgcgcc accgcaaggc
     1921 cttcttccat ggattgtatg gcgcgcggat ggtcgccgag agccaactgc gccaacgcac
     1981 gaacctctgc ggccggggca ccttggcatg tttcaatgac ttgtaatgct tgcgcggcat
     2041 gaccattgtc gagcatcgcc cgtgccagaa tcgcggaagc ttcgtccttg tgatcggtgg
     2101 ttacctcgga cttcgcgatc agagaagttg caagctgaat cgcttgttcg tttcgcccca
     2161 tccgacggag caaatcggcg cgaagcacct ctgctgatgt gtcagaacaa ccgtcgacca
     2221 cggcataagc agtccctaaa ccaccggcgc ttcgcatcgc tttcgccaat aaaacgcgat
     2281 ggatttccgg tagtgatcct ttccagagcc gctgctcggc tacttgcatc gcggccgccg
     2341 acacgggacg ttggacgagg gccagggcaa gcggaacgtc gtcttcaggg tcttgcccct
     2401 ttggggcaac gtcgtgaatg gcatcgtcat gcaagacacc gagaacgtcc aatagcgtcc
     2461 ataacgatgg gtctttgcga tgagcgagtc gttcgagcaa cgcggcaaga tcgcgttctg
     2521 aacgctgcgt gatcgtggag ggcaaagacc aatgggtggc gccaactccc accagcgcga
     2581 ccaaccagcg tttgatcccc gcggcgtcga ggggttggat atccaaggaa gtcgttgtgt
     2641 ggtttggcgc ggaaccatgt cgcagcgcgt gcgcggtgcg aacgatacgc acggcttgtt
     2701 cgagccacgg acagaccgat tcggaaatgc gcacctcgat ggtttgtcgt gcaagcgcca
     2761 gggtttgttt tcgggtgcgc aagtaggcgg ttcgaacgcg agccgggcga cgcgcgttgc
     2821 ttcggaaccc ggtgatccaa cgggaaatcc atgcggcgga agggcgtgcg gagggttgct
     2881 gcgcggtcaa tgcgcgaagc caagcccccc atggcgcgat caaatcgcgt gaccaaagcg
     2941 ttgtggcggg ggttgcggat tgagcgatgg ttgcgtcgag aacctcggcc aggcacaagc
     3001 cgaatcccca taagtcacgg gcgattgcgt tgcgctggcc gtgggcaagc tcaggtggcg
     3061 cgtatcgaag ggtgagcccc cacaagtcgt cggtgttggc ttcgaccgcg tagccaaggt
     3121 cgatgagcgt tgccgtgtac cctgtggcct gttggtcgat gaggatattg ttgggggaga
     3181 catcaccgtg ggccattccg gcttgatgca agtcgtcgag tgccgcgccc acgtctctgg
     3241 cgatcgccgt cgcgatggca atccgctggg agtccggtat ggattgtggg cacaagcgtt
     3301 ggccttggac ccaggacaag gctaagaaag cctggggttg gggggattgt gtttcggaat
     3361 gggggagttg gccgaggtcg agaacggaag gcaagtgcgg agaaccgaca agcagcagtg
     3421 attcggcttc atgggcgagc gcggcggcag aactcgggtc acgggcgagt ttgacggcgg
     3481 cgattgcgtt ggtttgcttg tctttggctc gccaaacgat accactggcg ccttggccga
     3541 cgacggtgtc gagggcgaag cgccaatgtt ccggtagatc aatcaactga gtttgcaatt
     3601 acacaaatat agctgcaatt tatgagtttg tcatcttgta cgactgtcaa ctaagtaggc
     3661 gatcgtcgtt acggggccac ccgccacact tgaacggcaa aagccccttg gcctcgttct
     3721 acggaaacca ccaactcgta ttcgcctgac gacggtgcgc atagcggacc gtggatgggc
     3781 gcgatggacc acgcttctgg gcctgtatcc catgcgatgg acgaaccgtc tggcgaattc
     3841 cagcccatat cgaggtcctg cacgccaggg cctcctacac cgaaagcccg atagcactgt
     3901 cctgcctgaa gcttgacgat gtatcgcgct tgctcgtcgg tggctccctg ttgtccggtc
     3961 aacacttccg aatgcgcgac catcccgagt gcgcttccac aacgcgctgt caatcgaacg
     4021 atatcctgat cgacatcccc aatcagttca aacccagccg tacactggga tggtgcctgg
     4081 ggctggtccg atgataacga attcacttga atccaaggca tcgtttccgc tctaggtaca
     4141 tggtcgagct tcgcgatatc ccccgtgcgc attttcgccg atgcatcgtg gccttcgatc
     4201 ctcgacagtt gcacggcgag tgggacggtg cagccttctt tgggcgaaga aggagccagg
     4261 gttgcgcatg atggcatgcg cccaaaagta tcccgttgtg tatcggccga aatcaccgcg
     4321 ttgaccgatt tttcggcccc aacgatgatt cggtaggtcc ccagggcgat ggagcgaacg
     4381 tagtgtgttg cggatgggca aacgatgctt gcagagcggg gagattcggt gtcgacaacg
     4441 aatcttccta cgatctgata gtgaaggcgc acaggggtgc ccgcttgaag cagagcaacc
     4501 agggcttctc cgctcgcggg aagcaactga cgagtggcct cgacgttcgg gagttcaatc
     4561 gttttggtga tgggtgcaac cgcttcataa cgatagggaa cctccaaagc acaacgtatc
     4621 catgtcgtct cccccgcggg ttgcgtcatg cggattgcaa atagctttcc tcggtatcgt
     4681 attggatcca ctgaagcagc tttggtgtct cgtaatggtc caagccagaa agaggagggc
     4741 gacgacgcca ctggggtgcc gcatgccacg atgcacaggc caagcaggct ggttgccgac
     4801 aaccttttga ccaattgcct tgtgatcggt ggacgacagt cgctggttgc tagtcgtttt
     4861 cctgtgcgac gaaacgaaag tctcatgtgc gaagtgcttt gcacaatagc agaacaatac
     4921 gctccttccg ggatttggtt ggggcgggct tttctccgtc cctcctgtct tgatatgatt
     4981 ctgccct
//
LOCUS       DAOSOR010000451         4969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_17060_length_4969_cov_3.961131, whole genome shotgun sequence.
ACCESSION   DAOSOR010000451 DAOSOR010000000
VERSION     DAOSOR010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17060_length_4969_cov_3.961131"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="PK710_19370"
     CDS             <1..1097
                     /locus_tag="PK710_19370"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HOT11948.1"
                     /translation="VTAHIGATGLNTDGSGAPIGPIVDFAKNINGADLVQGGHTEAEV
                     NWKMGDVMLVQSDGQLTAYNKVLITVEDGVVTDIQGSHVQVLGKKAITLSASCKDNTA
                     QCPTGFECVAEKLCEQVIEEPDPAVATMIEPWKTHVKELFDEKVGTVDGVWPRNGIVE
                     RSQEVPMGDIITDAILDSYAGLGIQIAFMNGGGIRDSMPSSYKPTDTSLVRVGCSTTT
                     PCDVVLGDIYSILPFNNTVVLRDVPGSVVWDVVEFSVSTVEANHGRFLQVGGIKVTYK
                     ASAAPGARVQSILFTGDGSNKPITKTDTTIYRIAVNDFMNTGGDGYEMLKEEVPTVPW
                     GLFNDAVADYIKKVKDLKTPEPFQRIIAIP"
     gene            complement(1347..2912)
                     /locus_tag="PK710_19375"
     CDS             complement(1347..2912)
                     /locus_tag="PK710_19375"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT11949.1"
                     /translation="MTQSVFLDRRGFITYLVGTSGLAFVGCSSESEEEQTIPRTPVTL
                     EPLDTVILTTERTIVPTKPHQTTLDPRSPIDPEGRALLIEDGYDEYEYGPGEPIIERM
                     PEGHTVGNPGAGRKVLTRFVHVTDIHVTDDESPIRLARFDGGHPLGGAARPHSSHMGR
                     ILNAAVRTVNALNRAQPIDFVLLGGDSSDSAQLNEMSWVIGVLSATGVIACDSGKTND
                     PVPGPDNDPKDPFVPEGLDIPWKFCLGNHDTEIMGISVITPLGVENSMGNNAVAGTQD
                     WSKPGGPVVVGEVPADENRRLLYRHEMLELVAADGDGHGLKNRPTSKASYTFDVGENL
                     RFIVYDTALEEGGADGIVRREDLEAFLKPALNKAKSEGKLVVLASHHGLGSLSDGGFG
                     NQSSKESAVLPDEVKEFFLSYGNIVMSITGHTHDHVVKWVGNNDDGFWEVQTCALVAF
                     PSQMRLVEIANEDNGYLSIQLICVDFATDNDVVAEQGRRLSILDYTSGWGGGDAGTPT
                     DRNVKLYVPLPSP"
     gene            complement(3036..3368)
                     /locus_tag="PK710_19380"
     CDS             complement(3036..3368)
                     /locus_tag="PK710_19380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11950.1"
                     /translation="MQALRADGTLASARIVELYGKLARVSFEDKTEAWQLAVELEPPA
                     TPASTPEDPCSVAPDDRIRAPWTLTNTMYAGRVRETHGRIAEVQFDDGDRTWVPCDAI
                     QPIESPSR"
     gene            complement(3533..4264)
                     /locus_tag="PK710_19385"
     CDS             complement(3533..4264)
                     /locus_tag="PK710_19385"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOT11951.1"
                     /translation="MISLLLDPIFVALLLLLIGLLVWTRSPPRRALLKGKIIAWMGFA
                     LLWIGASPWFSSRLSASLEPRPVDLTAALSDTEPSQRALVILAGGKRYDYDFLPSAER
                     LGPTTQARLLGAARIIREHGPFSLVIVSGTGMPFVTAMADYLTLHGVPKDSIVLEPKA
                     TDTTTNAVYSAAILQEHPKQRVVLVTSALHIPRSVAEFHKVGIEVVPAPVDYVGGRSF
                     QVIPSANSLSRTARCTHELLGHFEP"
     gene            4396..>4969
                     /locus_tag="PK710_19390"
     CDS             4396..>4969
                     /locus_tag="PK710_19390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOT11952.1"
                     /translation="MQQHGVEQVSHGQANDASSGWREEVHEIVSRICEESERTSRIIP
                     RDQLVFPSSVTEVMQAPGSVEQDTSPAHPIALATLRCACARPVFEQSARVTECVQDVL
                     EASARLRSVLGPGVERGAYRALLAHELRRRGLMVWEDVAVAASYQGMVIEEAYRADLI
                     VEGTTLVDLGREQGSRQEVEARLRGLMTKVG"
BASE COUNT         1075 a         1324 c         1502 g         1068 t
ORIGIN      
        1 tggtgaccgc tcatatcggc gccacggggc tcaacaccga cggttctggc gccccgattg
       61 gaccgatcgt agattttgca aaaaatatta acggtgctga tctggtgcag gggggacata
      121 cggaagcaga ggtcaactgg aaaatgggcg atgtcatgct cgtgcagagc gatggccagc
      181 tcaccgcgta caacaaagtg ttgatcacgg tggaagatgg tgtcgtcacc gacatccaag
      241 gttcccacgt tcaggttcta gggaaaaagg ccatcacact aagcgcatcc tgcaaagaca
      301 acacggcgca gtgtcccacc ggcttcgagt gtgtcgcgga aaaactatgc gaacaggtca
      361 tcgaagaacc ggaccccgcg gtggctacca tgattgagcc gtggaaaacg cacgtcaaag
      421 agctattcga cgaaaaggtc ggcactgtcg atggtgtttg gcctcgtaac gggattgtcg
      481 agcgctccca ggaggtcccc atgggagaca tcatcacgga cgccatcctc gattcctacg
      541 cgggtctcgg aatccaaatc gctttcatga atggtggagg catccgggac tccatgccct
      601 cttcctataa gcccacggac acctcgttgg ttcgagtcgg ctgctcaact accacgcctt
      661 gtgacgtggt gctcggtgat atctattcga tccttccctt caacaatacc gtcgtgctcc
      721 gcgatgtccc cgggtcggtg gtgtgggatg tcgtggagtt ttcggtgtcg accgtggagg
      781 cgaaccatgg ccgattcctt caagttggcg gcatcaaggt cacatataag gcttctgccg
      841 cccctggcgc ccgcgttcag tccattctct tcacgggcga cggttcgaac aagccaatca
      901 caaaaacaga taccaccatc tacaggatcg ccgtgaatga cttcatgaac acgggcggtg
      961 atggatacga gatgctcaaa gaggaagtgc ctaccgtccc gtggggcttg ttcaacgatg
     1021 ccgtggcgga ctacatcaaa aaggtcaaag atctgaaaac gcctgagccc ttccagcgca
     1081 tcattgctat tccgtaatcc cccatcccgc cttccaaaca ctcgacgctg acctcattca
     1141 ccacggtcga tgacgccctg agcgctgtcg tagcttggtc gaacctcgct tcccttgcca
     1201 aggtcccccc accagcccat accgttcggc accaccacgg taggaaagcg atgggcatga
     1261 taattgtaac taattgaaat aactgttaaa tttggatcgc cggcgcagcc tcgggcgatg
     1321 gatacgaacc attcggattg ggcgtatcag ggggagggaa gcggaacgta tagtttgacg
     1381 tttcggtcgg tgggggtacc cgcatctccg ccgccccaac ccgacgtata atcgaggatg
     1441 gacagccggc gtccttgttc cgctaccacg tcattgtcgg tggcgaagtc gacacagatg
     1501 agctgaatcg acaggtaacc attgtcctcg tttgcgattt ccaccaagcg catctgactt
     1561 gggaaggcga cgagtgcgca ggtttgtact tcccagaatc cgtcatcgtt atttcccacc
     1621 cacttgacca catggtcgtg ggtatgaccg gtaatgctca tgacgatgtt gccataagac
     1681 aggaaaaact ctttgacttc atcgggcaat accgcgcttt ctttcgagct ttggttgcca
     1741 aaaccgccgt cgctgagcga accaagcccg tgatgggagg caagaacgac aagcttcccc
     1801 tcggacttcg ccttgttcaa cgcgggcttc aaaaatgctt ccaagtcctc gcgtcgaacg
     1861 atgccgtcgg cgccgccttc ctccaaagcc gtatcataga caatgaaccg aaggttctct
     1921 cccacgtcga acgtgtaact cgccttgctc gttggacggt ttttcaaacc atggccgtcc
     1981 ccatcggcgg cgaccagctc gagcatctcg tgtcgataca aaaggcgacg gttttcgtcg
     2041 gcgggtactt ctcctacgac gacgggtcct cctggtttcg accaatcttg tgtgccggcg
     2101 acggcgttat tgcccatgga gttttcgacg cctaggggtg tgatgacgct gatgcccatg
     2161 atttcggtgt cgtggttccc gaggcaaaat ttccatggaa tatcgagccc ttccggaaca
     2221 aaggggtctt tgggatcatt gtctgggccg ggcaccgggt cgttggtttt gccagagtca
     2281 caggcaatca cgcccgttgc tgataagacg ccgatgaccc aactcatctc gttgagctgg
     2341 gcggaatcac tcgagtcccc tcccagcaag acaaaatcga tgggttgggc gcggttcaac
     2401 gcattgaccg tacgcaccgc cgcattcagg attcgaccca tatgcgaaga atgaggccgg
     2461 gcagcgccgc caagtggatg cccaccatcg aatcgcgcca gccgaatggg tgattcatca
     2521 tcggtgacgt ggatatccgt gacatggaca aatcgggtca gcaccttacg tcccgcgccg
     2581 ggattaccca cggtatggcc ttcgggcata cgttcgatga tgggttcccc tggtccgtat
     2641 tcgtactcgt cgtagccatc ctcgatcaac aaagctcgcc cttcgggatc gatgggactg
     2701 cgaggatcga gcgtcgtttg atgaggtttc gtaggtacga tggtgcgctc ggtggtgagg
     2761 atgacggtgt cgagaggctc gagagttaca ggtgttcgcg ggatggtctg ctcctcctct
     2821 gactccgaag aacatccgac gaaagccagg ccgctcgtac ccacaagata ggtgatgaat
     2881 cctctgcgat cgaggaaaac ggattgtgtc atgtgcggaa gcgtaacgcg gaatccgtga
     2941 tgatgcgatg ggattgaagc gaaatctggg ctttgatggg ctgattcgtg gttttactac
     3001 cccttgcatt gacgttcgat gagcaaggct caatatcagc ggcttgggct ttcgatgggc
     3061 tggatggcat cgcaaggcac ccacgttcga tcgccatcgt cgaattgcac ttcggcaatt
     3121 cttccgtggg tttcacgcac tctgccggcg tacatggtgt tggtgagtgt ccatggggcg
     3181 cggatgcgat catcgggcgc cacggagcat gggtcttcgg gagtgctggc aggtgtggca
     3241 ggcggctcca actccaccgc gagttgccag gcttccgtct tgtcctcgaa cgatacgcgc
     3301 gcgagtttcc catataattc cacgatccgc gcggaggcta gcgtcccatc ggcgcgaagc
     3361 gcttgcactt cgctaccgac ggtggagggc ggagttttcc gcccacatcc tcccagcgca
     3421 aacgcggcta agaatacgtg gacgagaaaa cgaaggctga gacgatcaag gaacagatgc
     3481 tgattccttt cgaaggatac ccaaaaagtg tgaccctgcc gcccacgaaa ggtcaaggct
     3541 cgaagtgtcc gagcaactca tgcgtacaac gtgccgtgcg agacaaggag ttggcggatg
     3601 gtatgacctg aaaactgcgc cctccgacat aatcgacagg agcaggaacg acttcgatgc
     3661 cgaccttgtg gaattccgcg accgatcggg gaatatgtaa ggcgctggtg acgagaacca
     3721 cccgttgttt gggatgctct tgcaggatcg ccgcggaata caccgcgttg gtcgtggtat
     3781 ccgtcgcctt gggctccagg acgatgctgt ctttcggaac gccgtgcagc gtaaggtagt
     3841 ccgccatcgc ggtgacgaaa ggcattcccg tgcccgagac gatgacgagc gaaaatgggc
     3901 catgttctcg aatgatgcgt gcggctccga gcagtctggc ttgggtcgtt gggccaaggc
     3961 gttcggcgga gggaaggaaa tcatagtcgt atcgttttcc gccagcgagt atgaccaacg
     4021 cgcgttggga tggctccgtg tccgaaagag cggcggtgag gtcgacgggc cgcggctcca
     4081 ggctggcgga tagacgcgac gagaaccaag gagatgcccc aatccataat agggcgaatc
     4141 ccatccacgc gatgattttc cccttgagca acgcccgtcg agggggggag cgggtccaca
     4201 ccaacaagcc gatgagcagc aagaggagcg caacgaatat gggatccaag agcaggctga
     4261 tcatggcgtg tgtaggttgg taccccacgg ggggcggatt cttcaacgat gaacggacga
     4321 agtgctgttt tgaggcaacc gcttgcttgt ctgtccgatg aaacggtgtg aatcacgatg
     4381 gtcgaaggag gagagatgca gcagcatggt gttgaacagg tatcccacgg tcaggcgaac
     4441 gatgcatcga gcggctggag ggaagaagtg catgagattg tttcgcgtat ttgtgaggaa
     4501 agtgaaagaa cgagccggat cattccccgt gatcaactcg tatttccttc ttcggttacc
     4561 gaggtgatgc aagccccggg ttcggtcgag caagacactt cgcctgctca tcccattgcg
     4621 ttggccacct tgcgttgcgc ttgtgcgcgg ccggttttcg agcagtccgc gagagtaacc
     4681 gaatgtgtgc aggatgtgct ggaggcttcg gcacggctga ggagtgtctt ggggcctggg
     4741 gtcgagcgag gggcctatcg cgcgttattg gcccatgagt tgagaaggcg tggtttgatg
     4801 gtgtgggaag acgttgcggt ggccgcttct tatcaaggaa tggtgattga ggaagcctat
     4861 cgagccgatt tgattgtgga agggacgact ctggtcgatt tgggcagaga gcaaggttcg
     4921 cggcaggagg tggaagcaag gctgcgaggt ctcatgacga aagtaggtg
//
LOCUS       DAOSOR010000452         4936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_17215_length_4936_cov_4.757837, whole genome shotgun sequence.
ACCESSION   DAOSOR010000452 DAOSOR010000000
VERSION     DAOSOR010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17215_length_4936_cov_4.757837"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PK710_19395"
     CDS             complement(<1..283)
                     /locus_tag="PK710_19395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11953.1"
                     /translation="MPLDRVEPEAAAAEEAVVKSAVVGAVLAQAAAAEVEAEPAAVVA
                     EAGPAAAVAEAEPAAAVAEAEPAAVVAEAEPAAAEAGAEPAAAVAEAQVA"
     gene            931..1854
                     /locus_tag="PK710_19400"
     CDS             931..1854
                     /locus_tag="PK710_19400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11954.1"
                     /translation="MKSIQTHACGVSGCVREIQVLGTLLRAGLQAIEPLTEVYKVDMS
                     DVAGLMKIVRSLRGNKLGMLSLSSSLSFQATSFADELGPSAERTSCVNMLMSTSAGIF
                     GHDIHYDTWQDRLAVIAPPRSTALILGTGPAAVAAVAACRQLDFQLIGITTRSWISTE
                     KLHDSPLADRFRQMGALPVLWPMSRECSASSRFSQQMRLQFGELASSAQALIQTIAVS
                     PSSEDARLLASVIPWTQTRQDAVVCDLIYGASTSPFLAEAQRRGLTGIGGIEILTMRG
                     LRIIETWTGQSPPRQLIHAAALDACARLSDE"
     gene            1847..3814
                     /locus_tag="PK710_19405"
     CDS             1847..3814
                     /locus_tag="PK710_19405"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOT11955.1"
                     /translation="MNDLFWPTLHVDGKLARARTEWIMGNGAGAYASSTVALMHTRRY
                     HGLLVAALEPPRKRTVVVSHMDIVLQDSGREVELASHQFPNVSPVEGYRHLQWFHQDP
                     LPRWTYQLTRGTFEESLALVRGINALVLRYLWRGDGAIHASARPLLAMRDHHTLTHAH
                     GAMIQRVHLRPREVCVQPIRNLPQVIFRHGGTFVGSPDWWHRFEYLDEQDRGLEFQED
                     LWTPGVFRLTFQPDVPQYLIVGLDKVPDEEGDVLFEQAAQALRNCDPGPSRSWPVRSL
                     FVAANLFRADECAFPSVIAGYPWFEVWGRHTLTALPGLYLIPGYIEQAKAVMKTLLGA
                     LRNGRIPNRFPETGESVEYHSADASLLLFRVARQLTEALPANDVFAQEVLLPALREVF
                     EAYDKGTEDGIHVTPAGLISAGNPDTSLTWMDARVGEQPVTPRWGAAIELQALWSKGC
                     DDLAWLASRCGDEQLASRASMARDQARSAFAKRFWCESTHYPFDVVSANEDLETAWAD
                     PTIRPNALIALAVDPDLFTTGQAQKIIERVENELLTEMGVRTLSPNDSRYRGFYRGGV
                     EDRDRAYHQGTAWPFLFGALVQATCHTFPNDEPRKARLRSLVENTLSKQIAIGQIPEL
                     ADGDAPHRSQGCVAHAPAVAEMLRALEMLDA"
     gene            complement(3835..4302)
                     /locus_tag="PK710_19410"
     CDS             complement(3835..4302)
                     /locus_tag="PK710_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HOT11956.1"
                     /translation="MDQNLLRSYLSTVYEIPSPSGLLRVSMDGDVAADPSLLPDPLTK
                     TFAILTAYNPRSMLLPRRVNEARHQVLRDLLILGVYRVESCVGYEDEPEGVWREPAWF
                     VHNMDRVEAEAFGRVFRQNTIVWSSGGRPELIVTDPTCDELGKTVAASFRVRS"
     gene            4714..>4936
                     /locus_tag="PK710_19415"
     CDS             4714..>4936
                     /locus_tag="PK710_19415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11957.1"
                     /translation="MLDFFLTAGVALFLGQLAPSSPWPTAMAAIGLVVIGWRRLSMTQ
                     TVVLFFFFALAAYRAGSMLNQYDEARVAAW"
BASE COUNT          988 a         1449 c         1405 g         1094 t
ORIGIN      
        1 gagccacctg tgcctccgct accgccgccg ctggttccgc ccccgcttcc gccgccgctg
       61 gttccgcctc cgctaccacc gccgctggtt ccgcctccgc taccgccgcc gctggttccg
      121 cctccgctac cgccgccgct ggtcccgcct ccgctaccac cgccgctggt tccgcctcca
      181 cttccgccgc cgctgcttgc gccagtaccg ctcccaccac cgccgatttc accaccgcct
      241 cctccgccgc cgctgcctcc ggttccactc gatccagagg cacaggcggg gcacgatgcc
      301 gaatagcaag attccaaggc ctgccaggct tgagcgcccg tggcgtgaat ttgtgaacag
      361 gaggaaaggc aggcgaggtc ttgtggatgg caagcatttc gacacagggc aagcgccaca
      421 caagcaggag tatttccgca tgcttctgcc tggctgcaac aggtttggtc gatacaggcc
      481 gcacattcgg agttgccgac ggcaacattt ccgcattgtg ttcccgcggg acgccccccc
      541 attcccccat cgggcggtac atcggattca aacgacactt cgtcgaactc gaggatgctg
      601 gagcaaccaa gaccaagagc ccctaccatg aaccaaccga taagggttcg accgtgtgtt
      661 ttcgtcatgg tctgatactc ccctttgaag cgctggattt cgaagcccta gagtatagca
      721 cgtcgtgcat cgtcagccag ccgttttcag catgaaattc gacgaatcgc tttggatagg
      781 gcgagccaac gaggcagaag ggcagcggac cgacgaagat attttcgtga aaaggcgacg
      841 gttttgtcga agggtccact ttcgatctcc ccttgggtgc gttctggtcg tacactcggc
      901 tatcgatcgg gttcaacgcg aatggggagc atgaagtcaa tccaaaccca tgcttgtggg
      961 gtcagcggat gcgtccgtga gatccaggtg ctggggacat tgcttcgcgc gggcttgcag
     1021 gcgatcgagc ccttgacgga agtgtataag gtcgacatga gtgacgttgc ggggctcatg
     1081 aagatcgtcc gctcccttcg tggaaacaag cttggcatgc tgtcgctttc cagttccctg
     1141 agttttcaag ccacgagctt tgcggacgag ctggggcctt cggcggagcg aacctcgtgt
     1201 gtgaacatgc tgatgagcac ttcagcggga attttcggtc acgacattca ctacgacacg
     1261 tggcaggaca ggcttgccgt gattgctcca ccgcggagta cggcgttgat actcggcaca
     1321 gggcctgcgg cggtggcagc ggtagcagcg tgtcgccagc tcgattttca actcatcggt
     1381 atcaccacgc ggtcttggat ttcgaccgaa aagctccatg attcaccgct cgccgataga
     1441 tttcggcaaa tgggggctct tccggtgcta tggccgatgt ccagggaatg ctcggcaagt
     1501 agccggtttt ctcaacagat gcggcttcaa ttcggggaat tggcatcctc ggcgcaagca
     1561 ctgatccaaa cgattgccgt aagtccatct tcggaagatg cacgactact agcatccgtg
     1621 atcccttgga cacaaacacg gcaagacgcc gtggtctgcg atctgatata tggggcctcc
     1681 acgagcccat ttctggccga ggcacaacgt cgcggcctta ctgggatcgg aggtatcgaa
     1741 atactgacca tgcgtggatt acgaatcatc gaaacctgga cagggcaaag ccctccgcga
     1801 caactcatcc atgcggccgc actcgatgcc tgcgcgagac ttagcgatga atgatctatt
     1861 ttggccgacg ttgcatgtgg atggaaagct tgctcgcgct cgcacggaat ggatcatggg
     1921 caatggggcc ggggcatacg catcttccac agtcgcgttg atgcatacac gtcggtatca
     1981 tggcttgctc gtagcagcgc tcgagcctcc ccgtaaacgt acggtcgtgg tttcccacat
     2041 ggatatcgtg ctgcaagaca gcggtcggga agtcgaactg gcaagccatc aatttcccaa
     2101 cgtctctccc gtggagggat atcgtcatct tcagtggttc catcaggatc cgttgcctcg
     2161 ctggacgtat caactcacca ggggaacgtt cgaggaaagc cttgcgttgg tacgaggcat
     2221 caacgcgttg gtgttgcgct atttgtggcg tggcgatggg gccatccacg cttcggcacg
     2281 tcctcttctc gcgatgcgtg accatcacac ccttacccac gcgcatggcg cgatgattca
     2341 acgggttcac cttcgccccc gcgaagtatg tgttcagccc attcggaacc taccccaagt
     2401 catttttcgt cacgggggca ccttcgtggg ttctcccgat tggtggcacc gattcgagta
     2461 tctcgacgaa caagatcggg ggctcgaatt ccaagaggac ttgtggactc ctggtgtgtt
     2521 tcgactgacc ttccaaccgg atgtccccca ataccttatc gtgggattgg ataaagtgcc
     2581 cgatgaggaa ggggatgtgc tttttgagca ggcggcacaa gctctgcgca actgcgaccc
     2641 tgggccatcg cgaagctggc ccgtgcgcag tctattcgtg gcagccaacc tatttcgtgc
     2701 ggatgaatgc gcatttccaa gtgtcattgc aggctatccg tggttcgagg tttggggtcg
     2761 tcacacgttg accgcgttgc cgggactgta cctcattcct ggatatatcg agcaagccaa
     2821 ggccgtgatg aaaacgttgc tcggagcctt gcgcaacggt cgaatcccca atcgtttccc
     2881 ggagacgggt gaatcggtgg aataccacag cgccgatgcc tccttgctgc ttttccgtgt
     2941 cgctcgacaa ctcaccgaag cattgcccgc caacgatgtc ttcgcgcaag aggttctact
     3001 tcccgcgctt cgtgaggtat tcgaagcgta tgacaagggc acggaggacg gcatccacgt
     3061 cacccccgcg ggtttgattt ccgctggaaa tccggacact tcgctcactt ggatggatgc
     3121 tcgggtgggg gagcagccgg tcacaccccg atggggcgcc gccattgagt tgcaagcctt
     3181 gtggtccaaa ggatgcgacg atctcgcatg gcttgcgtct cgttgcggcg atgaacagct
     3241 agcaagccga gcatcgatgg ctcgcgacca agcgagatcg gcttttgcaa aacgcttttg
     3301 gtgcgagtcc actcactatc ctttcgatgt cgtttccgcg aacgaagatc tggaaacggc
     3361 gtgggcggac ccaacgattc gacccaatgc attgatcgca ctcgcggtcg atccggattt
     3421 attcaccaca gggcaagctc aaaagatcat cgagcgagtg gaaaacgaat tactgacaga
     3481 aatgggcgtt cgaactttgt ctccgaacga ctctcgatat cgtggctttt atcggggagg
     3541 cgtggaagat cgagatcgag cttaccatca gggaactgcc tggccttttt tgttcggggc
     3601 gctcgttcag gctacctgcc acaccttccc caacgatgag ccgcgcaaag cgcgattgcg
     3661 ctctctggtc gaaaacaccc tttccaaaca aattgccatc ggacaaatcc ccgaattggc
     3721 agatggtgat gccccccatc gctcccaggg ttgcgtggcc cacgcccccg ccgttgccga
     3781 gatgctccgc gccctcgaga tgttggacgc ataactcgcc gtcagatacc cccttcagct
     3841 tcgaacccga aaactcgcgg ccaccgtctt tccaagctcg tcgcacgtcg gatcagtcac
     3901 aatcagttct ggccgccccc cagacgacca tacaatcgta ttctgtcgaa atacccgccc
     3961 aaaggcttcg gcctccacac gatccatgtt gtggacgaac catgcgggct ctcgccacac
     4021 gccttccggt tcatcttcat atcccacgca cgactccacc cgataaactc ccaaaatcaa
     4081 cagatcccga aggacctgat gcctggcctc gttcacgcgt cgtggcaata gcatcgaacg
     4141 tgggttgtac gccgtcagaa tcgcaaaggt tttggtcaat gggtcgggaa gcaagcttgg
     4201 gtccgcggcc acatccccat ccatcgatac gcgaagcaga cccgatggcg acggaatctc
     4261 ataaaccgtg gacagatagg aacggagcag gttttgatcc atgagcgttg atcttccgga
     4321 cttggcgtgg agatagcact cgtactcgac gtcgaattgt caggtcaagg gatcggggtt
     4381 gtggagccga cggcggcgat tgcggtttgt cccatcccct caggagtgat cgacggctga
     4441 tggcaatggg tgatgacgac cgatgacgga gttattctta gaaaactggg gtgtttgggc
     4501 gcgggggcag tgctgagtgg cgagcgccaa tttgggggtg gcgcccgcca tatccttgta
     4561 actatctaaa attacacatt aaattactgt tactccagct tctgggtgct tcgtggggtg
     4621 gcgcaccgcc agtcgtacca tcggttgcga tcatgaaatc aagggaatca tcaagcaacg
     4681 tcgagtggcc caggtcttga tacggcgcgg gtcgtgctcg atttttttct cacagcaggc
     4741 gtcgcgctgt ttttgggtca gcttgctccc agttccccct ggcctacggc catggcggcg
     4801 ataggtctcg tggtgattgg atggcgtcgg ctttcgatga cgcaaaccgt ggtgttgttt
     4861 ttcttttttg cgctggcggc ctatcgagct ggcagcatgc tcaaccaata cgacgaggct
     4921 cgtgttgccg catggg
//
LOCUS       DAOSOR010000453         4921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_17269_length_4921_cov_4.545417, whole genome shotgun sequence.
ACCESSION   DAOSOR010000453 DAOSOR010000000
VERSION     DAOSOR010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17269_length_4921_cov_4.545417"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="PK710_19420"
     CDS             <1..998
                     /locus_tag="PK710_19420"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT11958.1"
                     /translation="GDHQVPVAIKRILSHIARDPAIVALFHEEARVGRQLAHPNIVQV
                     LDDGKDDAGNDYLVMEWVEGLDLYDYMRSYHAARVHVPWQAVAAIGFQILRGLAAAHE
                     RVDGQGQRRPVIHRDLTPSNILLGVDGVVKVADFGLARATDRATMTFPNIIKGKVSYT
                     APELLYGHKANEQSDVYSLGVTLWEALAARKLYSGKNYSEVLAAIQFGEVPSLSKLRP
                     DCPAELIRIVLQALERDVQKRWKTARQMAEPLLAFVRSIRPSVDARRLGGSVQQARDR
                     LRAIDGKLPVIMQEASSDSSIEISIEVSVDEETRSMPAREFRAELSDPFAAVDED"
     assembly_gap    1104..1113
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1232..1915
                     /locus_tag="PK710_19425"
     CDS             1232..1915
                     /locus_tag="PK710_19425"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOT11959.1"
                     /translation="MPQKRASTPTNDTAPVATGTGKRISLGTVERLSVYRSVLEELHE
                     DGVASVYSHQLAEMVGVTPAQLRRDLASFGSFGNIARGYPVFEMARTISGILHTDTMQ
                     VLALFGVGDLGRALLTYRGFEERGFRIGVVFDIDPAKAGKVFAGRRCYHLDQLESALP
                     DFDVRIAVLACRPAGVEGIVERLGRLGVRNILNFVPKRVKPVPGGYVEHVDISGKLEK
                     LSFLSSQRR"
     gene            2018..2443
                     /locus_tag="PK710_19430"
     CDS             2018..2443
                     /locus_tag="PK710_19430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT11960.1"
                     /translation="MNTHHILLVDDDRDLLLSIQAFLTPRGYRVSCAASGAEAKQLIP
                     SSRPDLIVLDVMMESDTEGLVLAQELQRDPQTQSIPVILLTGFMEHLDEKRQTMLFAL
                     DQPWPGAKLLEKPVHLDTLASTIERLLEERRVLDAAKEG"
     gene            2445..2891
                     /locus_tag="PK710_19435"
     CDS             2445..2891
                     /locus_tag="PK710_19435"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOT11961.1"
                     /translation="MPSSSTPGPEALIPLLQQAQARDGFLSPSVMTDIAKLLGIPVSR
                     VFGVATFYNQFRLAPLGEHVIEVCRGTACHVKQSLTLYQALERRLRLRADGNSPDGKF
                     SVVTVACLGACSMAPVLRLDGQFYGHLTVESLDALLTELEQSQRAA"
     gene            2919..4916
                     /locus_tag="PK710_19440"
     CDS             2919..4916
                     /locus_tag="PK710_19440"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOT11962.1"
                     /translation="MMASSELLSPCCSKCKHSPADPCRDRIPCLQRGPLCHDDDSCRS
                     LKSARWERARRGGTGTILWVGAGTCGRANGALRVLDTIKTYLQDRNIEAQVVQVGCLG
                     NCQQEVLVDIHVDGSPRFCYGNIGVDDVPGLLDSVLAQGEYRHPRLIGRHGGPEVGFE
                     DIPEVFHTPYFALQTRNVLERCGLLDPESLDAALSWRAFEAAARALTTLTPEEVCDEV
                     ERAGLRGRGGAGFPTGKKWRVALRTPSAQKYLICNADEGDPGAFMDRALLESDPFRII
                     EGMLIAAYAIGATQGYVYCRAEYPLAIERLEKAIAQCREAGLLGKNILGSLVDFDITI
                     KQGAGAFVCGEETAILHSIEGGRGMPRPRPPFPATSGLFGKPTILNNVETLANVPSIL
                     LRGADWFHSLGLDGAAGTKVFALSGAVKNTGLVEVPVGTSLRRIVGEIGGGAPEPHRI
                     KAVQIGGPSGGCIPEALLDVAVDYGALQRLGAMMGSGGLVLMDERSCMVDVAKFFMEF
                     VRNESCGKCAPCREGTTRMHEILTLVAERPVGSDLRRLERLQAMLYLEELATTVRDAS
                     LCGLGQSAPNPVLSTLRFFREEVEAHILEGRCPAGACQGLRVYEIDNERCIGCLLCKK
                     QCPSGAIVGERKHAHYIVVDSCVGCGSCVEVCPKDAIHRAA"
BASE COUNT          970 a         1286 c         1549 g         1106 t
ORIGIN      
        1 agggcgacca ccaagtgcct gtcgccatca aacgaatcct atcgcatatc gcccgggacc
       61 cagccatcgt cgcgttgttt catgaagaag cgcgggtagg tcgtcagctt gcccatccca
      121 atatcgtaca ggtgttggat gatggaaaag acgatgcggg caacgattat ctggtgatgg
      181 aatgggtcga aggtctggat ttatacgact acatgcgatc gtatcacgcg gcccgggtcc
      241 atgtgccgtg gcaggccgtg gcggccattg ggttccaaat cttgcgcggt ttggcggcag
      301 cgcatgaacg agtcgacggc caaggacaac gtcgaccggt cattcatcgg gatttgacac
      361 ccagcaatat tttgttgggt gtggatggtg tggtgaaggt ggcggatttt ggcctggcgc
      421 gggccacgga tcgagcgacg atgacctttc cgaacatcat caagggaaag gtttcttata
      481 cggctccgga gctgttgtat ggccacaagg cgaatgagca aagtgatgta tacagtttgg
      541 gggtgacgtt gtgggaggct ttggccgcgc ggaaactata ttcggggaaa aactattcag
      601 aagtgcttgc cgcgatccag tttggcgagg tgccgtcgct atccaagctg cgaccggatt
      661 gtcccgcgga gctgattcga attgtgcttc aggcgttgga acgggatgtt cagaagcgat
      721 ggaagacggc acggcagatg gcggaacctt tgcttgcgtt cgttcggtcg atacggcctt
      781 cggtggatgc gcgaaggctt ggcgggagtg tgcagcaggc tagggaccgg ttgcgagcga
      841 tcgatggtaa gttgccggtg atcatgcaag aggcgagcag cgatagttcg atcgagataa
      901 gcatcgaggt atcggtcgac gaggagactc gatcgatgcc ggcgcgtgag tttcgggcgg
      961 aattgagtga tccgtttgcg gcagtggatg aggactgacg tgcaggcggg ggggcgatgc
     1021 ttgcgggtga tgggttttgg aggggggtgg gtaagggggg ggctgggggg gcttgggggg
     1081 gggctgtctg ccccccccgc cgcnnnnnnn nnnggggggg ggtggggggg gctggcgcat
     1141 cggaggacaa gcatttttgc gcggcatgca gaaatggttt gacaaagttt tgtctgtgaa
     1201 tattatcaca gctagctgaa ggaacggaaa cgtgccccaa aagcgagcat ctaccccaac
     1261 gaacgatacg gcaccggtgg cgacgggcac ggggaaacga atttctttgg gaacggtgga
     1321 gcgtctttcg gtgtaccgct cggtgctcga agaactccac gaagatggcg tagcgtccgt
     1381 gtactctcac caactcgcgg aaatggtggg ggtgaccccc gcccaattgc gtcgggatct
     1441 cgcgagcttt gggtcttttg gcaacatcgc tcgcggctat ccggtgttcg agatggcgcg
     1501 aacgataagc gggatccttc atacggacac gatgcaagtg ctggcattat ttggcgtggg
     1561 cgacttgggg cgagctttgt tgacgtatcg tggattcgag gagcgaggct ttcgtatcgg
     1621 cgtcgttttc gatatcgacc ctgcgaaagc gggaaaagta ttcgcggggc gacgctgtta
     1681 tcatctggac cagctcgaaa gcgcccttcc tgatttcgat gtgcggattg ccgtcctggc
     1741 gtgccgtccc gccggtgtcg aaggtatcgt ggagcgtttg gggcgcctag gagttcgtaa
     1801 catcttgaat tttgtgccaa aacgcgtgaa gccggtgccg gggggctatg tggagcatgt
     1861 tgatatttca gggaaattgg aaaaactatc gttcttgagc agccagcgtc gctaaccgtt
     1921 gcctcgctcc tctgctctat tctttcgttc cttattgtga aaaaaatcac tgtgaaaccg
     1981 tgaacgaaat cggtatcaac cccatcggag gccaaccatg aacactcacc atatcctgct
     2041 cgtcgatgac gaccgcgact tgctcctttc catccaagct ttcctcacgc caagaggata
     2101 tcgtgtctcc tgcgccgcct ccggtgcaga agccaaacaa ctcatcccct cctctcgccc
     2161 agacctcatc gtcctcgatg tcatgatgga atccgacacc gaaggactcg tcctcgccca
     2221 agaactccag cgtgaccccc aaacccagtc catcccggtc atcctgctca cgggctttat
     2281 ggaacacctc gacgaaaagc ggcaaaccat gctctttgcc ctcgatcagc cctggcccgg
     2341 agccaaactc ctcgaaaaac ccgtccacct cgataccttg gcctccacca tcgaacgtct
     2401 cctcgaggaa cgtcgggtgc tcgacgccgc gaaagaagga tagcatgccg tcttcctcta
     2461 cacccgggcc cgaagccttg attcccttgc ttcagcaagc tcaagccaga gacgggtttc
     2521 tttccccctc cgtcatgacg gatatcgcga aattactcgg gattcctgtc agtcgtgtct
     2581 ttggagtcgc caccttttat aaccaatttc gactggctcc cctgggagaa cacgtgatcg
     2641 aagtatgtcg ggggacggct tgtcatgtga aacagagtct tacgttatat caggcgttgg
     2701 agcggcgtct tcgcttgcgg gccgatggaa attcccccga cggcaagttc tctgtcgtga
     2761 cagtggcctg tttgggcgcc tgttccatgg cacctgtcct acggcttgat ggccagttct
     2821 atggccatct taccgtggag tcgttggacg cgctgctcac cgagttggag caatctcaac
     2881 gggcggcatg agcctgccgt tgcgaatcag ggagatgcat gatggcaagt agtgagcttc
     2941 tttccccctg ttgttcgaaa tgcaagcatt cgcccgcgga cccttgccgt gaccggattc
     3001 cctgtcttca gcgagggccg ctctgccatg acgatgactc ttgtcgttcg ctgaaatcgg
     3061 ctcgttggga gcgtgcccga cggggtggaa ccggcacgat cctatgggta ggcgcgggga
     3121 cgtgtggtcg agcgaacggg gcgttgcggg tgctcgatac aataaaaact tatttgcaag
     3181 accgaaacat cgaggctcag gtcgtgcaag tgggatgttt gggaaactgc caacaagaag
     3241 tgctggtgga tatccatgta gatggaagtc cacgtttttg ttacggaaat attggtgtcg
     3301 acgatgtccc ggggctgttg gattccgtgc tggcgcaggg ggaataccgt catccccgct
     3361 tgattggccg ccatggagga cctgaagtgg ggttcgagga tattcccgag gtatttcata
     3421 ccccttattt tgctctccag acgcgaaatg tattggaacg ctgtggtctt ttggatccgg
     3481 agagtcttga tgcggcgttg tcttggaggg ctttcgaggc ggcggcgcgg gcccttacca
     3541 cactgacccc cgaggaagta tgcgacgaag tggaaagggc gggactgcga gggcgagggg
     3601 gtgcgggatt tcccaccggg aaaaagtggc gcgtggcctt gcggacccca agtgcacaaa
     3661 aatacctgat ttgtaatgcc gacgaggggg atccgggggc tttcatggat cgggcgctgc
     3721 tggaaagcga cccgtttcgc atcatcgaag gcatgctgat tgccgcgtat gccatcggcg
     3781 ccacccaggg atacgtctat tgtcgtgctg aataccccct tgcgatcgaa cgcctggaaa
     3841 aggccatcgc tcaatgcagg gaggccggcc tgttagggaa aaatattctc ggatccttgg
     3901 tggacttcga tattaccatc aagcaagggg cgggagcttt cgtgtgcgga gaggaaacag
     3961 ctatcttgca ctccatcgaa gggggacgcg gcatgccccg tccaagaccg ccgtttcccg
     4021 ctacctctgg cctttttggc aagcccacca tcctcaataa cgtggagacg ctggcgaatg
     4081 taccgtcgat cctgcttcgg ggcgccgatt ggtttcacag cttgggcctt gatggcgctg
     4141 cggggacgaa ggtctttgcc ctcagcggtg ccgtgaaaaa caccgggctg gtcgaagtgc
     4201 ctgtcgggac ctccttgcga cgcatcgtag gcgagattgg aggcggtgcg ccggagccgc
     4261 atcgcatcaa ggccgtgcaa atcggaggcc ccagcggcgg ctgcattccc gaggctttgc
     4321 tcgatgtcgc ggtggactat ggagccttgc aacggctcgg cgccatgatg ggatccgggg
     4381 gattggtgct catggacgag cgttcgtgca tggtcgatgt tgccaagttt tttatggaat
     4441 tcgttcgaaa cgagtcctgt ggcaaatgcg cgccctgccg agaaggcacc acgcgcatgc
     4501 acgaaatcct gacgttggtg gcagagcgtc cggtcggctc cgatcttcgt cgcctcgagc
     4561 ggcttcaagc catgctgtat ctcgaggaac tggccaccac cgtccgtgac gcttcccttt
     4621 gtgggctcgg tcaatccgct ccgaacccgg tcttgtccac ccttcggttt ttccgtgaag
     4681 aagtggaagc ccacattctc gaaggtcgct gccctgccgg tgcgtgccag ggattacgag
     4741 tctatgagat tgataatgag cgctgcattg gctgcttgct ttgcaaaaaa caatgccctt
     4801 ccggcgccat tgtcggcgaa cggaaacacg cccattacat cgtagtggat tcctgcgttg
     4861 gttgtggaag ttgcgtcgag gtttgtccca aggacgccat tcaccgcgcg gcttgagccg
     4921 c
//
LOCUS       DAOSOR010000454         4921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_17270_length_4921_cov_4.219688, whole genome shotgun sequence.
ACCESSION   DAOSOR010000454 DAOSOR010000000
VERSION     DAOSOR010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17270_length_4921_cov_4.219688"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="PK710_19445"
     CDS             <1..879
                     /locus_tag="PK710_19445"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOT11963.1"
                     /translation="YQFISINKRNVLRGCSTSLHASAADERIRCNFIPSFATPWVAPQ
                     HFVLTLGPIMFGRPIRHPWYDWPEPLEPPSRAHPSNPWRTSPVRARTPRPVQPPETEI
                     SGDGDVPIRGKPFLDPSDAPTPLGSDDMPTTPATPTPSRDEQLRNALLELEESKKRLD
                     RERDKERERVRADLVVQLLPVLDNLDRSIQASAQRDTQDPLREGVRMVRDQFDEVLQG
                     FGLEVFGTAGTAFDPAIHEAMALVAVDDPSQHRNVMEIVRAGYRLEGRLLRAAQVIVG
                     DYRAPEVEQSPTSEEQ"
     gene            complement(882..2054)
                     /locus_tag="PK710_19450"
     CDS             complement(882..2054)
                     /locus_tag="PK710_19450"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOT11964.1"
                     /translation="MPTGSILILVLMAVALVITFLFWRHLQPSIDAPKPSSKPSSKPS
                     DQKKPSSKPSDKNAPDGSVSGKDAPRSDAIPSLVLQRDRDSDPEITMMGKVPEDVLAL
                     LQGKLSADVPIEEEEAFDIDVQELVSETAEEEITGKHALILVVGVARSDRGRKRQCNE
                     DAFLIVEEQHLFVVADGMGGHNAGEIASQKAVDTIGECFSNNVFPGEPKPDWPRRGDE
                     LVRAIHDANDKVLELAQSRDDYSGMGTTVVAVRFSPNKQRAYIAHVGDSRCYRIRHDE
                     LHQLTEDHTLGRLMGATGKTARHLAQAVGVRPEVAVDLTVDEPQPGDHYLVCSDGLTK
                     MVPDSRLLEIATIDENLDLRCQQLIDEANERGGRDNITVIMVRVESPLKTDNEPSA"
     gene            2238..3182
                     /locus_tag="PK710_19455"
     CDS             2238..3182
                     /locus_tag="PK710_19455"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOT11965.1"
                     /translation="MNASAFDQQNGALGWEPIGPIATRRKADEWRFVLRAVDVGSWVA
                     DTQDRQFVLMVPSEQADRALRELAEYEQENRDRIRERPFRDVPLYRDTRWALAIVAAL
                     ALFFSITGPSHSRSAWFQVGIADTDAILSGAVYQTVTALTLHSNTQHLVGNALVGGLL
                     FAIVHRRLGPGLGSLVILMAGASGNLLNAVWHATEHRSLGASTAVMGALGVLASTQFV
                     LNQVQRSNAKAVIKWAPFVAGAALLGTFGASPTSDLHAHGFGFLCGLLLGLIAAFPLR
                     HRKQPLPLWSQILFGLTALLVITGAWTTAFLLGHATPV"
     gene            3258..4280
                     /locus_tag="PK710_19460"
     CDS             3258..4280
                     /locus_tag="PK710_19460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11966.1"
                     /translation="MGQVDQRLEQLHHSLQAEIASMLQRHEARVRGYDAVDLDLGRGT
                     IIFSNNNNTLWTGRVELIGTFLPDTNAWRWWWFGADRPLTRSPLNAVFAAGQSHGIRT
                     LTSAQPAIRGKHEAVQLANLCAALAKSQGVHVVQNADRMTFYGLFNLRSSVIVAGREP
                     SPPPPEPDSSVVHHDSLITVGEPLSPIITTRQPIMSMPPPAYGPLPPVRPPPQKTPIS
                     EPTSVPSASDNEVRPPQRALVMPLAKVASTAVITHLPSGFQQALITLDLHVQQGTKAR
                     FFVVITALDTEGNLVAVPTPHDLMEAAKDLILEDARQGNGRWRRLTVRLTNRPMGISV
                     HVEVVA"
     gene            4261..4677
                     /locus_tag="PK710_19465"
     CDS             4261..4677
                     /locus_tag="PK710_19465"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HOT11967.1"
                     /translation="MSKWWPEVHSQDRNTANTPVVMQRRQRHQAMVWGLLVLGLGGML
                     GVSFLVSPDDIDEGRVALTPACPTKQWFGMPCPTCGMSRAFASLSRGQWGEAMRYNRA
                     SPIVYVLTWTGLVWGSMNSLMSLHKARRFRKKDVRR"
     gene            4674..>4921
                     /locus_tag="PK710_19470"
     CDS             4674..>4921
                     /locus_tag="PK710_19470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11968.1"
                     /translation="MSFQPPPGFGPPREPDNTAPPDIRRFQPPPGYGSPAPGIPAAGG
                     PGFGVQTNTMAITSLVTGILSIPLHFCCYLGWPAGIVA"
BASE COUNT          996 a         1476 c         1335 g         1114 t
ORIGIN      
        1 taccaattta tttccataaa taagcggaac gtgcttcgtg gctgtagtac tagcctacac
       61 gcgagcgcgg cggatgaacg aattcgctgc aatttcatcc cgagcttcgc gacgccgtgg
      121 gttgcccccc agcatttcgt ccttaccttg ggtcccatca tgtttggcag accaattcga
      181 cacccctggt acgactggcc tgaacctctc gaacctcctt cccgtgccca tccctcgaat
      241 ccctggcgta cgagccctgt tcgagcaaga acaccacgcc ccgtccagcc gcctgaaacg
      301 gaaatctccg gtgatggcga cgtccccatc cgcggaaaac cattcctcga tccctcggat
      361 gcaccaactc ccttgggttc ggatgacatg ccgacaacgc ctgccacgcc aacgcctagc
      421 cgcgacgagc agcttcgtaa cgcattgctc gaacttgaag agagtaagaa acgcctggac
      481 cgcgaacgcg acaaagagcg cgaacgtgta cgagcagacc tcgttgtcca acttctgccc
      541 gtgctcgata acctggatcg ttccattcaa gcttctgccc agcgggatac ccaagaccca
      601 ttgcgcgagg gcgtcagaat ggttcgagac caattcgacg aagtgcttca aggttttgga
      661 ctcgaagtat tcggaacggc tggaactgcc ttcgatccag ccattcacga agccatggct
      721 ctcgtcgccg tggatgatcc ttcgcaacat cgaaacgtga tggaaatcgt tcgcgcggga
      781 tatcgcctgg aaggccgttt gctccgcgcc gcccaagtca tcgttggcga ttatcgagca
      841 ccggaggtcg agcaaagccc gacaagcgaa gaacaataac ctcaggccga tggctcgtta
      901 tccgttttca acggagattc cacacgaacc atgatgacgg taatgttgtc gcgtccccct
      961 cgttcattgg cctcgtcaat gagttgttga caacgaagat cgaggttttc atcgatggtg
     1021 gcgatttcca gaagacggct gtctggaacc atttttgtca gtccatccga acaaaccagg
     1081 tagtgatctc ccggctgcgg ctcgtcgaca gtgaggtcca cggcgacttc gggacggaca
     1141 ccgaccgctt gcgccagatg gcgcgccgtt ttgcctgttg ctcccatgag tcttcccaac
     1201 gtgtggtctt cggtgagttg gtgaagttcg tcatgacgga ttcgatagca acgactatcg
     1261 cccacatgcg ctatataagc gcgctgtttg ttcggggaaa aacgcacggc caccacggtc
     1321 gttcccatgc cgctatagtc gtctctcgat tgcgccaact cgagtacttt gtcattcgcg
     1381 tcgtgaatgg cacgaaccaa ttcgtcgcct cgacggggcc aatcgggctt tggttcgcct
     1441 ggaaaaacat tgttggaaaa acattctcca atggtgtcga cggctttctg gcttgcgatc
     1501 tcaccggcgt tatggccgcc catgccatcg gcaaccacga ataggtgctg ttcttcgaca
     1561 atgagaaaag catcttcgtt acattgccgt ttgcggccac ggtcgcttcg cgccactccc
     1621 acgaccagga taagagcgtg ttttccggtg atctcctcct cggcagtttc ggaaaccaac
     1681 tcttgcacat cgatatcgaa agcttcttcc tcctcaatgg gcacatcggc cgagagcttt
     1741 ccctgcaaaa gcgccagaac gtcttccggc acttttccca tcatggtgat ttcgggatcg
     1801 gaatcacgat ccctttgcaa aacaagggat gggatcgcat cagaacgagg cgcgtccttg
     1861 cccgaaacag aaccgtccgg ggcgtttttg tccgacggct tggacgatgg tttcttttgg
     1921 tccgacggct tggacgatgg tttcgacgat ggtttgggcg catctattga aggttgcagg
     1981 tgtcgccaga acagaaaagt gatgacaagg gcgacagcca tcaggacaag gatgaggatc
     2041 gaaccagttg gcatggtttg ctgatggcca ccataccttt tcgtgctgct cttgcgtgta
     2101 ctttcttgga gtgtgccgcg tccctgacgg caggctgcgg ttgacaaggg gaaaaccatc
     2161 cccaacttcc ttgcttcaat gccttggttg ctctgccacc gattcccatc acgtcgattg
     2221 tttcgttcga ggacggcatg aacgcgagcg ctttcgacca gcagaacggg gcattgggtt
     2281 gggaacccat cggtcccatt gccacgcgga gaaaggccga tgagtggcgt ttcgtgcttc
     2341 gggcggtcga tgtgggaagt tgggttgccg acacgcagga tcggcaattc gtgcttatgg
     2401 tgccttccga acaggcggat cgggcgttac gggagttggc cgaatacgag caagaaaacc
     2461 gagatcgcat tcgtgagcgg ccttttcgcg atgttcccct ctatcgagat acccgctggg
     2521 cattagcgat cgtcgctgcg ctcgcactgt ttttttccat cacaggccca agtcattcgc
     2581 gaagcgcctg gtttcaagtc ggaatcgcgg acaccgacgc catcttgtcc ggggcggtgt
     2641 accagacggt gaccgcgttg accttgcatt cgaatacgca acacttggtg ggcaatgcgc
     2701 tggtgggagg tcttcttttc gccatcgtac atcgacgttt gggccccggc cttgggtccc
     2761 ttgtcattct catggcagga gccagcggaa acctgctgaa tgcggtttgg catgccacgg
     2821 aacaccgaag tctcggcgca tccacagcgg tcatgggcgc gctgggagtt ttagcctcca
     2881 cacagttcgt gttgaatcag gtgcagcggt ccaatgcgaa agccgtcatc aagtgggcgc
     2941 cttttgtcgc gggagccgct ttgctcggca cgtttggcgc ctcccctacc tccgatctgc
     3001 atgcccatgg tttcggcttt ctgtgtggtc tgctactcgg cttgatcgcc gcttttccct
     3061 tacggcatcg aaaacaaccg ttaccccttt ggtcgcaaat cctcttcgga ctcaccgccc
     3121 ttctggtcat caccggagct tggaccaccg cattcctgct tggtcacgca acccccgtat
     3181 gagtgggtct tgcacttacc catacggccg tggtttgcta ttccgcgggc tgctgcttga
     3241 accacgacga ccaccacatg ggccaggttg accaaagact cgaacaactg caccactcgt
     3301 tgcaagcgga gatcgcgagc atgctccaac ggcatgaggc tcgcgttcgc ggatatgacg
     3361 cggtggacct cgaccttggt cgaggcacta tcatttttag caataataac aatacgttat
     3421 ggacaggaag ggtcgaactt atcggcacct ttctgcccga taccaacgcc tggagatggt
     3481 ggtggttcgg cgccgatcgg ccgctgactc gttctccgct caatgccgtg tttgccgcgg
     3541 gacaaagcca cggaattcga acgctgacct ctgctcaacc ggcgatacga gggaaacacg
     3601 aggccgtcca actcgccaat ctatgcgctg ctctcgccaa atcccaaggc gtgcatgttg
     3661 tgcaaaacgc cgatcgaatg acgttttacg gtctgttcaa cctccgttcc tccgtcatcg
     3721 tggctgggcg tgaaccgagc ccaccaccac cggaacccga ttcgtcggtc gtccatcacg
     3781 atagtctcat cacggttgga gagcctcttt cgcccatcat tacgactcgg caaccgatca
     3841 tgtcgatgcc accaccggcg tatggcccct tgccccccgt gcgccctcct ccccaaaaaa
     3901 cgccgatttc tgagccaacc tcggttcctt cggccagtga caacgaggtg cgaccgccgc
     3961 aacgcgctct cgtcatgccc cttgccaagg tcgcttcgac cgcggtgatc acgcacttgc
     4021 cctcgggttt tcaacaagcg ctgatcacgc tcgatctgca tgtgcaacaa ggaacgaaag
     4081 ctcggttttt cgttgtcatc acagcgttgg acacggaagg aaacctcgtg gccgttccaa
     4141 cccctcacga tctgatggaa gccgcaaaag acctcattct cgaggacgca cgacaaggca
     4201 acggtcgatg gcgaagattg accgtacgct tgaccaaccg tcccatggga atctccgttc
     4261 atgtcgaagt ggtggcctga ggtgcattcc caagaccgaa acactgccaa tacgccagtg
     4321 gtcatgcagc ggcgtcagcg tcatcaagca atggtttggg gcctgctggt ccttgggcta
     4381 ggtggcatgc tcggcgtatc cttcctcgtt tctccggatg atatcgatga aggccgtgtg
     4441 gctctcaccc ctgcttgtcc caccaagcaa tggttcggca tgccatgccc aacctgcgga
     4501 atgagccgag cttttgcgtc gctcagccgt ggacagtggg gtgaagcgat gcggtacaat
     4561 cgcgcttccc cgatcgttta cgtgcttaca tggacggggt tagtatgggg aagcatgaat
     4621 tcgctcatgt cgttacataa agcccgacgc ttcagaaaaa aggatgtgag gcgatgagct
     4681 ttcagcctcc cccgggattc ggcccccccc gggagccgga caataccgct ccaccggata
     4741 tcaggcgttt tcaaccgccc cctggatatg gaagcccagc tcccggtatc cctgccgccg
     4801 gaggacccgg ctttggcgtg caaaccaaca ccatggccat tacctcgctg gtgacgggaa
     4861 tcctcagcat tcctctgcac ttctgctgtt atctcgggtg gcccgcgggt atcgtcgcca
     4921 t
//
LOCUS       DAOSOR010000455         4914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_17322_length_4914_cov_3.794608, whole genome shotgun sequence.
ACCESSION   DAOSOR010000455 DAOSOR010000000
VERSION     DAOSOR010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17322_length_4914_cov_3.794608"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="PK710_19475"
     CDS             complement(<1..822)
                     /locus_tag="PK710_19475"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOT11969.1"
                     /translation="MMERTTDERPKTAICLTGGGVTGGAYQLGALSALESVVESEPFD
                     LIIGTSSGASVAAAIAGGLPVSRLFRALLDPADNFFPLERNHILSLDVGEWRRTIMSG
                     LNAIRHGVASLVARSPAPMPSDLWEQLDRLFDALPAGLLSLDRYEHFLADFFLRRRVP
                     NSFRSLHRELLITATDLDGGESVLFGSSQFDHVPISLACTASMALPLFFSPVRIGDRY
                     YVDGSVAPTTEIDIAVANGAKFILVLNPNVPIRVSVMPDGVPTGHGIRSSLRDKGL"
     gene            complement(819..1682)
                     /locus_tag="PK710_19480"
     CDS             complement(819..1682)
                     /locus_tag="PK710_19480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOT11970.1"
                     /translation="MLDDDSEDRLSGIPPQERPLSVVAAVLWFVVATAGAMTLIAVTH
                     ALRPGAQTDIVNAVACQAIAYFATILLAVRVHGGNRALADIFALRKTHPIFFLLGGLL
                     GVCLQIPAELLQRLVFRFLPMDPQSLEMQSEMLRMDSLVSRIMIPIVVIGLGPFVEEL
                     FFRGLLFGGMRRSHGVGFTCFMVAVLFAGAHGSIQLFVPLLVIGGAITLVRAVSGSVG
                     PGLVAHFCFNAIPILGIAFGWIRFDSDPTPLPWTISVGGVLATMLILAAFVMLSLRSQ
                     AAVRARQEDLR"
     gene            complement(1742..2170)
                     /locus_tag="PK710_19485"
     CDS             complement(1742..2170)
                     /locus_tag="PK710_19485"
                     /inference="COORDINATES: protein motif:HMM:NF013995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HOT11971.1"
                     /translation="MAYWLLKTEPGAYSFDDLLRDKGTCWDGVRNYQARNNLSRMKQG
                     DRCVIYHSVGPKEAVGVAKVTKTAYPDPTSADAKWLAVDIVPEERFAFPVSLATMKAH
                     PVLAGMALVRQSRLSVCPVTSDEFEALVSLANIARPSNRI"
     gene            complement(2199..4196)
                     /locus_tag="PK710_19490"
     CDS             complement(2199..4196)
                     /locus_tag="PK710_19490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11972.1"
                     /translation="MARSLVRSAAVVCGVATLSISSACSLTDPLGGYADGQGKPSGSD
                     AGTDVVNDVHVYACPKGFDDCDGLASNGCETNTNSSLEHCGGCHQPCLVEQGVGICLL
                     GQCQIDSCLPGFDDCDGEVQNGCEADLSDVAHCGFCGNVCEFDNAQAVCEQATCLIDH
                     CQPGFDDCDLDEDNGCEQDIHSSLEHCGECGKPCLPRPNSTPSCEKGSCLLKCDKGYG
                     DCDGDHANGCESELGADVMNCGDCGHACSTAHAAPQCIGGKCVLTCEDGFASCDDDVT
                     TGCETDIGSDPNHCGSCETDCPSAPGAQPSCVSRTCGLACLSGLGDCNAKDSDGCETD
                     LTTHELHCGVCGRSCWGEECIEGTCPSRLLVKGLNRPTAMVLDATHVYWTDAYDNSVY
                     SMPKSGGDKSTVASEGATGPITIYGGILYWATGLGIAKVAVNGGAKKSVVDGESLVVD
                     LTNDGSTLFWLRPGTWSGSAYNEDGKVVRASMSGGSVLELSVDQKMPKALVVDSTHVY
                     WVNEGTYSGLKYNNDGSVMKVAKTGGVVTTLAYAQSAPCGIAIDNTTVYWANCRDGSV
                     WAVPKTGGVATALVQGQTVSEGLVISGGELYWPYRGPSPNAATGDVKKAWIGASQPLV
                     LAKSQAQPIRLAVDSLYVYWLNWGFDGDPPPGNGSVSRVAR"
     gene            4410..>4914
                     /locus_tag="PK710_19495"
     CDS             4410..>4914
                     /locus_tag="PK710_19495"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT11973.1"
                     /translation="MTSLSPSSPSTAHRVIGRYAIHHEIASGGMATVHLGRLLGEAGF
                     SRTVAIKRLHEHLAKDPDFVAMFLDEARLASRVRHPNVVSILDVVVLDKEVFLVMDYV
                     QGASVSRLARSARTGDKRIPPKITLSIMVGALHGLHAAHEAIGENGQSLGIVHRDVLA
                     DAIGPLLH"
BASE COUNT         1192 a         1570 c         1195 g          957 t
ORIGIN      
        1 caatcctttg tcgcgaaggc tcgatcgaat gccgtgcccc gtaggaacgc catcgggcat
       61 aaccgatacg cgaatcggca cgttcgggtt caaaacaagg atgaattttg ctccgttggc
      121 aacagcgata tcgatctccg tcgtgggtgc cacgctgcca tccacgtagt accgatcacc
      181 gatacgaacg ggtgaaaaaa acagcggcag cgccatcgaa gccgtacagg cgagagaaat
      241 gggaacgtga tcgaattgcg aagatccaaa caatacgctt tcaccgccat cgagatcggt
      301 tgcggtaatg agcaactcac gatgtagact tcgaaaactg ttcggaacac gtcttcgtag
      361 gaaaaaatcg gctaggaagt gttcgtatcg gtccagggac agcaaccccg cgggcaatgc
      421 atcgaagaga cgatcgagtt gttcccaaag gtcagaaggc atcggagccg ggcttcgcgc
      481 cacaagcgaa gctacgccat gccgaatggc gttcaatccg ctcatgatgg tgcgccgcca
      541 ttcgccgaca tccaggctca atatgtggtt ccgctcgagg ggaaagaagt tgtccgcggg
      601 atcgagcagc gcgcgaaaaa gcctggatac cggcaaacct cccgcaatgg cggcagccac
      661 gctggcgccg ctggacgtgc caatgatcaa atcaaaaggc tcggattcca ccacggattc
      721 gagcgcggac aaggcgccca attgatacgc gccccccgtc acgccccctc ccgtcaaaca
      781 aattgcggtc ttcggacgct catccgtcgt tcgttccatc atctcaagtc ctcctgacgc
      841 gcacgcacgg cggcctgact gcgtaaagaa agcatgacaa acgctgcaag gatcaacatg
      901 gtcgccagca ctcctccgac gctgatcgtc caaggcaagg gagtggggtc actatcgaaa
      961 cggatccatc cgaaagcaat gccgagaatc gggatcgcat tgaaacaaaa atgagccacg
     1021 agaccaggtc ccacagaacc gctcacggct cgaaccaacg tgatggctcc cccgatgacc
     1081 agcaaaggaa cgaataattg gatactccca tgggcacccg cgaaaaggac agccaccatg
     1141 aagcaagtaa accctacccc atgactgcgc cgcatacccc caaacagaag accgcgaaag
     1201 aaaagctcct ccacgaaagg ccccaaacca atgaccacga tcggaatcat gattcgtgag
     1261 acaagactgt ccatgcgaag catttcggat tgcatttcca gcgattgcgg atccatcgga
     1321 agaaagcgaa acaccagccg ctgcaacaac tccgcgggga tctgcaaaca aacgcccaat
     1381 agccccccca acaggaaaaa aatcggatgc gtcttgcgca aggcaaatat atccgccaac
     1441 gccctgttgc caccatgaac gcgaaccgcc agcaagatcg tcgcaaagta cgcgatggcc
     1501 tgacatgcca cggcattgac gatgtccgtc tgcgcacccg gacgcaacgc atgcgtcacg
     1561 gcaatgagcg tcatcgctcc tgccgtggcc accacaaacc acaacaccgc cgccacgacg
     1621 gacaacggtc tttcttgcgg aggtatcccg ctcaaccgat cctcagagtc gtcgtccagc
     1681 atggtcccga ttcccttagt acaggtatgg ggaagttggt atccgtccga gcggtgagcg
     1741 ttcagattcg attcgaaggt cgagcaatat tggcaagaga aaccaatgct tcgaactcat
     1801 cggaggtaac tggacaaacc gaaagacgag actgtcgcac caaagccatg cccgccagca
     1861 cgggatgagc cttcatcgtt gccagagaga ccggaaacgc aaaacgctct tcaggcacga
     1921 tgtctaccgc caaccatttc gcatccgccg acgttgggtc tgggtaggct gttttcgtca
     1981 cttttgccac gccaaccgct tccttcgggc ctacggaatg gtaaatcaca caacgatcgc
     2041 cctgtttcat gcgcgaaaga ttgttgcgag cttggtagtt tcgcacgcca tcccagcacg
     2101 tccccttgtc tcgcaacaaa tcatcgaaac tatacgcacc gggctctgtt ttcagcagcc
     2161 aatacgccat ggtccgtcgt ctccatccgc acgattacct accgggcaac gcgcgatacc
     2221 gagccattgc ctggaggtgg gtcgccatca aacccccagt tgagccagta cacgtacaga
     2281 ctatccaccg ctagccgaat cggttgcgct tgcgacttcg caagaacaag gggttgcgac
     2341 gctcctatcc aagctttttt tacgtcgcca gtagccgcgt tagggctagg tcctcgatac
     2401 ggccaataca attctccccc ggatatcacc agaccctcac tcacggtctg accttgcacc
     2461 aaggcagtcg ccacacctcc cgtttttggt accgcccaca ccgaaccatc acggcaattc
     2521 gcccaatata cagtggtgtt atcgatcgcg atgccacacg gcgcactttg cgcatacgcc
     2581 agggtagtaa ccaccccccc cgtcttcgcc actttcatca cggaaccatc gttgttgtac
     2641 ttcaatcccg aataggttcc ctcattgacc caatatacgt gcgtggaatc gaccaccaac
     2701 gccttgggca ttttctgatc caccgaaagc tcgagcactg atcccccaga catcgaggcc
     2761 cgcacgactt tcccatcctc gttgtacgca ctgccgctcc aagttccagg acgcagccaa
     2821 aacaacgtcg aaccatcatt ggtcagatcg acaacgagac tttcgccatc gaccacactt
     2881 ttctttgccc ccccattcac agccactttc gcaattccaa gacccgtcgc ccaatacaat
     2941 atcccgccgt aaatggtgat cggaccagtc gctccctccg atgccaccgt cgacttatcc
     3001 cctccagact tgggcatgct gtacacactg ttgtcgtacg cgtccgtcca atacacatgc
     3061 gttgcatcga gcaccatcgc ggtcggacga ttcaagccct tgacgagcaa acggctcggg
     3121 catgtccctt cgatacattc ctccccccaa caactcctgc cacaaacacc gcaatgcagt
     3181 tcatgcgtcg tgaggtccgt ctcacaacca tccgaatcct tggcgttgca atctcccaag
     3241 ccggacaaac aagccagacc acacgttcgg gagacacacg agggttgtgc tcccggtgcc
     3301 gacgggcagt ccgtttcgca tgagccacaa tgattcgggt ccgatccaat atccgtttca
     3361 cagcccgtgg taacgtcgtc atcgcagctt gcgaacccgt cttcacacgt caatacgcat
     3421 ttgcctccga tgcattgagg agcagcatga gccgtcgagc aggcatgacc gcaatcgcca
     3481 cagttcatga catccgcgcc caactccgac tcacacccgt tggcatgatc gccatcgcaa
     3541 tccccataac ctttgtcgca cttcaataag cacgagccct tttcgcaaga tggtgtcgag
     3601 ttcggccgtg gcagacatgg ttttccgcac tcgccgcagt gctcaaggga agaatggatg
     3661 tcctgctcgc acccattgtc ctcgtccaaa tcacagtcgt cgaaacccgg ttgacaatgg
     3721 tcgataaggc aggtggcttg ttcacaaacg gcttgcgcgt tgtcaaattc gcagacgttc
     3781 ccacagaacc cgcaatgcgc aacgtcagac aagtccgcct cacacccgtt ttgcacctcc
     3841 ccatcgcaat catcgaatcc cggaaggcaa gaatcgattt gacactgtcc aagcaagcaa
     3901 attcccaccc cctgctccac cagacagggc tgatggcagc ctccacagtg ttctaagctg
     3961 gaattcgtgt tcgtctcgca tccgttgctc gcaaggccgt cacaatcgtc gaaccctttg
     4021 gggcaagcat agacatgcac atcgttgacg acgtcagtac ccgcatcact cccggacggt
     4081 ttgccttggc catcggcgta tccaccgagt gggtccgtca aggaacaagc cgatgaaatc
     4141 gaaagagttg ccacgccgca aacgaccgcg gcgctacgaa ccaatgaacg cgccattacc
     4201 aaaaggatgt cgcagttgcg tagcgccgtc aacgaagggt tacgatggga actccaccac
     4261 gaactaacgt catggcaccg cggacccatc cacgggcgcc cggtccgaag tctggaatta
     4321 cgattctttc tcacgagtgc ccatcgcaaa taacaatgga gtgcgacggc tttgcggtca
     4381 tggtgtccaa tcccgtatac taacagcgcg tgacatctct ttctccttcc tcgccttcca
     4441 ccgcacaccg agtcatcgga cgatatgcca tccatcatga gatcgcgtcc ggaggcatgg
     4501 ccacggtgca tctcggaaga ctccttggcg aagcgggttt ttcgcgaacc gtagcgatca
     4561 aacgcttgca tgaacacctg gccaaagacc cggatttcgt cgccatgttt ttggacgaag
     4621 cccgtctggc ttcccgcgta cgccatccga atgtcgtatc gatcctcgat gtggtcgtgc
     4681 tcgacaaaga ggtgttcctc gtcatggact acgtccaggg cgcctcggta tcacgtcttg
     4741 ctcgctccgc ccgcaccggc gacaaacgga ttcccccaaa aattacgttg agcatcatgg
     4801 tcggtgccct tcatggactg cacgctgctc atgaagccat cggcgaaaat ggacagtccc
     4861 tcggcatcgt tcaccgtgac gtgcttgccg atgcaattgg tccactgctg cacc
//
LOCUS       DAOSOR010000456         4909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_17342_length_4909_cov_4.826741, whole genome shotgun sequence.
ACCESSION   DAOSOR010000456 DAOSOR010000000
VERSION     DAOSOR010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17342_length_4909_cov_4.826741"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PK710_19500"
     CDS             complement(<1..644)
                     /locus_tag="PK710_19500"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT11974.1"
                     /translation="MKQSQAPPGAGGFPKRFGKYTLLRPLATGGMAELYLALQKSVAG
                     FEKLIVIKRILPSMNSDPAFIDMLLHEARIAATLSHPNVVQIFDVGFAEGAFYIAMEH
                     IHGEDLRSIVHQMKKLRMTEFPLEHALAIVVGMCAGLAYAHEKHGLDDEPLRIVHRDI
                     SPQNVIVTYSGDIKIVDFGIAKSGEGMGEDTKSGRLKGKVPYMSPEQARGEEID"
     gene            869..1390
                     /locus_tag="PK710_19505"
     CDS             869..1390
                     /locus_tag="PK710_19505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11975.1"
                     /translation="MRWIIASMVCVATVGTFGCATYLDDLNRAERHYQSNHHEQALAL
                     FRALEPDIDSLSHADRARYAYLRGLNDYRLSGHQDESAVTTETPSATSGDKAFRAHAR
                     YWFGIARALEKQRPGSLRKEWMDTLDEALRDLNQDVFGVGVFPDDTDSPSTDSKPEDS
                     NTKSSETDEPDES"
     gene            complement(1398..1616)
                     /locus_tag="PK710_19510"
     CDS             complement(1398..1616)
                     /locus_tag="PK710_19510"
                     /inference="COORDINATES: protein motif:HMM:NF016231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HOT11976.1"
                     /translation="MRRIGAADSDTLATLMAEHRALDERICQLGRQRFLTPDEQLEIR
                     TLKKLKLAKKDRIAVLERRDSFDRRGNP"
     gene            1673..2914
                     /locus_tag="PK710_19515"
     CDS             1673..2914
                     /locus_tag="PK710_19515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT11977.1"
                     /translation="MTKDHLLMIAQLAPNTIQAKKGDETMTRITQNVKNGRLVGLSLL
                     LLGCAGGVESQQPARVAGTQPAMTLPTVYVTADDAGTVSVRMQKANALLLQGQWASAA
                     EAFDRIVSLEPDGPHAPAALFNGGFAWEQIGREKEALERYRKLIDRFADRQETLGALI
                     RASRLSSRGEDWNSLLQLGNTMLSRPDLSIVDRIEALGARGMGLAQQGDWKGAMLSIG
                     KARGLIEEQGWLDTGKLTVGVAQVFFALGEVLRMQGEQILFDPRPADFSEALEQRCQS
                     LLDAQDAYATAMRAYDSHWSAMAGYRVGRMYQQLHADIMRIRPPASAKTERQKELFEG
                     AMQLRYRVLLDKGLKMMDSTIAMAQRTGESSSWVQRAKEAQRDIQRAYDTANAALAKL
                     PFSEQDLQRALDQMAGKPDSP"
     gene            complement(2965..4059)
                     /locus_tag="PK710_19520"
     CDS             complement(2965..4059)
                     /locus_tag="PK710_19520"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT11978.1"
                     /translation="MPHPFPHRLTIFSPHITPTPRILFWETTGACNLRCVHCRREWTD
                     APPNELSTDEVKRLIIDAIAQTNWHSILVFSGGEPLMRPDLEELIAYCTTQGVVPAVA
                     TNATLVDDLVAQKFKQLGVSRVSVSLDGANAKTHDAFRGLHGSFDDALRGIRSLRKAG
                     VDVQINVTFTKANRDEASDMLDLVQMLDACALHVFLLVPVGCGVELPDDVRLDAAEVE
                     RLLTFFDEQASERDLEVRATCAPQSQRIRQQRARARQRKGLPEARGLAPSSKGCLAGG
                     GILFIDAVGKVFPCGYLPMQAGDLRTTPLKTILETSELLSSLRDVTKLEGRCHGCEYD
                     QICGGCRARALGTTGRLFDEEPSCPMNMIR"
     gene            4349..4421
                     /locus_tag="PK710_19525"
     tRNA            4349..4421
                     /locus_tag="PK710_19525"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Thr,seq:tgt)
     gene            4486..4568
                     /locus_tag="PK710_19530"
     tRNA            4486..4568
                     /locus_tag="PK710_19530"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4520..4522,aa:Tyr,seq:gta)
     gene            4617..4692
                     /locus_tag="PK710_19535"
     tRNA            4617..4692
                     /locus_tag="PK710_19535"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4650..4652,aa:Gly,seq:tcc)
     gene            4707..4779
                     /locus_tag="PK710_19540"
     tRNA            4707..4779
                     /locus_tag="PK710_19540"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4740..4742,aa:Thr,seq:ggt)
BASE COUNT         1051 a         1328 c         1486 g         1044 t
ORIGIN      
        1 caatcgatct cttccccacg agcctgttcg ggggacatat agggaacttt gcccttgagt
       61 cggccgcttt tcgtgtcttc tcccatccct tcgccgcttt ttgcgattcc aaaatcgacg
      121 atcttgatgt ccccggaata ggtaacgatc acgttttgcg gagagatgtc gcgatgcacg
      181 atacgtaacg gttcgtcatc gaggccatgt ttttcatggg cgtacgcgag ccccgcgcac
      241 ataccgacga caatggcgag agcgtgttcg agcgggaact ccgtcatgcg gagttttttc
      301 atttgatgga cgatggagcg caggtcttcg ccgtgaatgt gctccatggc gatgtagaaa
      361 gcgccttcgg cgaatcctac atcgaagatc tgcacgacat tggggtgcga aagggttgcg
      421 gcgatgcgtg cttcgtgcag cagcatatcg atgaacgccg gatctgagtt catcgagggc
      481 aggatgcgtt tgatgacgat gagtttttcg aatcctgcaa cgcttttttg aagagcgaga
      541 tacagttccg ccatccctcc ggtcgcaaga gggcgcagca gtgtgtactt cccgaatcgt
      601 ttggggaacc cccccgcgcc ggggggcgct tgggactgct tcatggctgg gcctgacgcc
      661 tccgtcatgt cgttctccta ccgttcaccc atgcgcatgc aaccatctcg cttaaaatgc
      721 cacactccgc acagatgtca ctggagcgtt ggaactgcaa cccccgactg ggcgattcgg
      781 cctgggacgg tgtcggtggg aaagaaagtg caacctacca acaggtagct tgattccatc
      841 gcctattgtt cgataggctg cgaaggtcat gcgctggatt atcgcatcca tggtttgtgt
      901 tgcgacagta ggtacgttcg gttgtgcgac ctatctggac gacttgaacc gtgcggaaag
      961 gcattatcag tccaatcacc acgagcaggc gcttgcgtta tttcgcgctc tcgagccgga
     1021 tatcgactcg ttgagccacg ccgatcgcgc tcggtatgcc tatttgcgag gtctcaatga
     1081 ctaccgtctc agcggtcacc aggatgaatc cgccgtgact acggaaacgc cctcggccac
     1141 ttccggtgac aaagcgtttc gcgcccacgc tcgctactgg ttcgggatcg ctcgcgccct
     1201 tgaaaaacaa cgtccgggtt ccctgcgcaa agaatggatg gataccctcg atgaggcgct
     1261 gcgcgacctc aatcaagatg tattcggcgt aggcgttttc cccgatgata cggacagccc
     1321 atcgaccgac tcgaagcccg aggattccaa cacgaaatcg tccgagacgg acgaacccga
     1381 cgagtcgtga tgcccgttta ggggtttccg cgccgatcga aggaatcacg acgttccaat
     1441 acggcgattc tgtctttttt tgctaacttc agtttcttga gggttcgaat ctcgagttgt
     1501 tcatcgggcg tcaggaatcg ttgtcgaccc aattggcaga tacgttcatc gagcgcacgg
     1561 tgctcagcca tgagcgtggc cagagtgtcg ctatcggctg ccccaattct tctcatggtc
     1621 gatacctccg tggcggaagg tgtctgacga ccgaggcgat gttggtcgta tgatgacaaa
     1681 agaccatctg ctcatgatcg cgcagttggc cccgaacacg atacaagcca agaagggtga
     1741 tgaaacgatg acgcgtatca cgcagaacgt caagaacggt cgactggtgg ggttatcgct
     1801 gcttttgctt ggctgcgcgg ggggggtgga aagccaacag cccgcgcggg tagcgggaac
     1861 gcaacctgcc atgacgcttc cgacggtcta cgtaacggct gatgatgcgg gaacggtttc
     1921 ggtacgaatg cagaaagcca atgcgttgct actccaaggt caatgggcga gtgcggcaga
     1981 ggcattcgac cgaatcgtat cgttggagcc cgacggtccc cacgcgccag cggcgttatt
     2041 caacggcgga ttcgcatggg agcaaatcgg tcgcgaaaaa gaggcgctcg aacgatatcg
     2101 caagctcatc gatcggtttg cagaccgaca agaaaccctc ggcgcgttga ttcgagccag
     2161 ccgcttgtcc agccggggcg aagactggaa ctcgcttttg caattgggaa acacgatgtt
     2221 gtcacgtccc gatctgtcca tcgtcgatcg aatcgaggct cttggcgctc gcggcatggg
     2281 acttgctcag caaggcgact ggaaaggcgc gatgctatcc attggaaaag cgcgtgggtt
     2341 gatcgaagag caaggatggc tcgataccgg aaagctcacc gtcggcgtcg cccaggtatt
     2401 ctttgccttg ggcgaagtac ttcgcatgca aggagaacaa atcctattcg atccaagacc
     2461 ggcggacttc tccgaggcgc tggagcaacg ctgtcaatcg ctgctcgacg cacaggatgc
     2521 gtatgcgacg gcgatgcgtg cttatgactc ccattggtcg gcgatggcag ggtatcgtgt
     2581 gggacgcatg tatcaacaac tgcatgctga tatcatgcgg atcaggccgc ctgcgtccgc
     2641 gaaaaccgag cggcaaaaag agttgttcga aggagccatg caacttcgtt atcgcgtgtt
     2701 gttggacaag gggctcaaga tgatggacag cacgatcgcc atggcgcaac gaaccggaga
     2761 atcatcatcg tgggtccagc gggccaagga ggcccagcga gacatccaac gggcatacga
     2821 tacggccaat gccgcgcttg ccaagctccc tttttcggaa caggacctgc aacgggccct
     2881 ggaccagatg gcaggcaaac ctgactcgcc gtaagtgcgg atgcggtctg cgccgcacgc
     2941 cgaaaccaag ctccggagcg tgattcaacg aatcatattc atcgggcagg aaggctcttc
     3001 atcgaataga cgaccggtcg tgccgagcgc gcgtgctcga catcccccac aaatctgatc
     3061 gtactcgcaa ccatgacatc gtccttccag tttggtgaca tcgcgtaacg acgacagcag
     3121 ttcggaagtt tcgaggatgg ttttgagcgg tgtggttcgt aagtctcccg cctgcatggg
     3181 caggtatccg caggggaaaa ccttgccgac ggcatcgatg aacaggatac cgccaccggc
     3241 caggcagcct ttcgaggagg gggcaagacc tctcgcctcg ggcaagcctt tgcgctgtcg
     3301 cgcgcgggcg cgttgctgac ggattcgctg cgattggggt gcgcaagtcg cgcggacctc
     3361 gaggtctcgt tcgctggctt gctcatcgaa aaaggtgagc aatcgttcga cttccgcggc
     3421 atccaatcga acgtcatcgg gcaattcgac accgcagccg accggtacga gaagaaagac
     3481 atgcaacgcg caagcgtcga gcatctgcac caagtcgagc atgtcagaag cctcatcacg
     3541 attcgctttg gtgaaggtga cgttgatttg cacatcgaca cccgcttttc gaagggagcg
     3601 aattccgcgc agcgcatcgt cgaacgagcc atgcaatcct cgaaacgcgt cgtgggtttt
     3661 tgcgtttgcg ccatcgaggc tcacgctgac gcgtgacacg ccgagttgct tgaatttttg
     3721 tgcaacaaga tcatccacga gggttgcgtt ggttgcgaca gcaggaacga caccttgggt
     3781 ggtgcaatag gcgatgagtt cttcgaggtc gggacgcatg agaggctcgc ccccggagaa
     3841 aacgaggatg gagtgccaat tggtctgcgc gatggcgtcg atgatgagcc gtttgacctc
     3901 atcggtcgac aattcattcg ggggagcatc ggtccattct cgtcggcaat gaacgcagcg
     3961 aaggttgcat gcgccggtag tttcccaaaa caggatccga ggggttgggg tgatatgggg
     4021 ggagaagatg gtgagccgat gggggaatgg atgaggcatg ggtggtggta ttcgggtttg
     4081 gggttgcgaa gctgactgtg gtgtgggaga gttgcgagcg caagtccgcc gagtaggctc
     4141 gggtcatgca tggatatctg cattttaggt gcatgaaatc gcatgccatc gcctgcagga
     4201 gcctcaactt gcacagaaag tgcatgggct aaaaagatct ccttgacacg cgaggtactc
     4261 ggagctacgc ctacgcctcc cttggcgcgc cccagcggtt cgtcttgggg ccagttggtc
     4321 cacatcaacg tggactgggt tttcccaggc tggcgtagct caatcggtag agcaccggtt
     4381 ttgtaaaccg gatgttaggg gttcaagtcc cctcgctagc tctgcaatat ctagcagcgt
     4441 ggcgcgagcc ccaaggagaa gacggaccaa tcgcttaaat cgttcggtgg gttgcccgag
     4501 tggccaaagg gagcagactg taaatctgcc ggcgcacgcc ttcggtggtt cgaatccacc
     4561 acccaccacc ccattggacc catgaatcaa cacacggaag gaatgatcga tcgtatgcgg
     4621 gagtagctca gttggtagag cgtcagcctt ccaagctgaa cgtcgcgggt tcgaaccccg
     4681 tctcccgctc cacctcacca ttatcagccc acctagctca gtaggtagag cacgtccttg
     4741 gtaaggacga ggtcaccggt tcgagcccgg tggtgggctc cgcagcgtaa gtcccgtcat
     4801 gggccttgcg tgttgcgtgt tttgtcgact gcgttcgatc tcacttggag agaccgtcat
     4861 ggcgaaggag aagttcgtaa ggaacaagcc gcacgtaaac gtgggcacc
//
LOCUS       DAOSOR010000457         4908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_17346_length_4908_cov_5.820729, whole genome shotgun sequence.
ACCESSION   DAOSOR010000457 DAOSOR010000000
VERSION     DAOSOR010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17346_length_4908_cov_5.820729"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PK710_19545"
     CDS             <1..448
                     /locus_tag="PK710_19545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11979.1"
                     /translation="LDRRLSLEAGRTEALFVSHESTMPRERLSSDNHAMTSVRASETP
                     GRTVSTVAWTALGAGAALGIGGYFAFAKADDVAKDSAFVCAHSLRCDPDRVDVVRRWD
                     AAALGMWVGSAVGVGVGLGLILAGQPNQSSTAWVVTPTQLQARTVF"
     gene            448..666
                     /locus_tag="PK710_19550"
     CDS             448..666
                     /locus_tag="PK710_19550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11980.1"
                     /translation="MPRRIAFAALVSVSVGCSFDIFHDTSWPNACDLDPSLSGCSDGG
                     PGDGDTEDRAEQDEAPLTSEGGDGDSNG"
     gene            834..3272
                     /locus_tag="PK710_19555"
     CDS             834..3272
                     /locus_tag="PK710_19555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT11981.1"
                     /translation="MATKVLVFESDGAFANELTTEFQALGCEVDVVDDGNIGLQMAAS
                     HRPDLILLSIELPRTNGFSVCNRIKKDPSLKDIPLIIMSSMSNKDTFEQHRRLRTRAE
                     DYLHKPIAFADLLQRVQQFLEISPSAGPSSLEDAIIIDDDISFEENDDEDLHTVMMNR
                     SSLEAFETVEPKAPATTAVSTTPSRPRPSSRAPSQMEVDDLDVDEFADAAFDRLLDAP
                     EPSDQKPSQTALQTPSQIAADTMSTEVPPMLLQEGIETRPATIPALPDIHSEELERLR
                     NELERMQTKAVRLEQDLSASRADNRRLEEQVRSTEQASAEVERLQRQIDELKSRASIP
                     PGRTGTVSSREFLDLREALNNKDKEILALRDQMSQKEKELLAARDVSLSLEREKADTQ
                     DKLLEFEKQALDFRMSIDRLTVQHGEEQQKSADLQARLNASLQQIEEQHTRITEQEAL
                     RLRESEEHAATLETQKREANQALDNLRGERETLSTRITELEKDLSDRNNRITGLENDI
                     KARDSRITELEGNVGDRDNRIVGLENDIKTRDSRITELEGIVGDRDNRIVGLENDIKT
                     RDSRITELEGIVGDRDNRITGLENDIKTRDSRISELEGNVGDRDNRIAGLESDIKTRD
                     SRISELEGNVSDRGRRIDELDASVRDHQSRLEQRDAQIKELQEQVRDREAKLADGESR
                     AKEKDARIDGLEQDLRAARQRTEEVEAERNERDSRIRDLESSLAKIITDRDALAEAKR
                     DLSEKRSELEVQLGASVEKVGQLEALVLAEQERAKKLQVKWDHDKASLDRAKNALAAV
                     LLQIEEVEARGLEG"
     gene            complement(3284..4606)
                     /locus_tag="PK710_19560"
     CDS             complement(3284..4606)
                     /locus_tag="PK710_19560"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT11982.1"
                     /translation="MIVSDGHIVACDPQETVVRGDIVIRNGRILAIGPDARRRAHASV
                     EIVDASGCAIIPGLIQTHVHVCQTLMRGMGEDLPLLTWLQKRVWPLEAAHDERSLHAS
                     AELSLAELVLGGTTTILDMGSTHGYDVILDACLRFGIRVFGGKVMMDRGRGVPGALRE
                     STKASLAQSDALCAHWDGANHGRIRYAYAPRFILSCSERLIRETVERMQAGSFLHVHA
                     AEHGEEKKAVRRAFGADDVDVLSRWGAKGPRCVLAHGVQLTARQVRSVAAQGTRVVHC
                     PSANLKLGSGIAPLDELMQAGVAVGIGADGAACNNNLDAWMEMRLAGLLAKLRKGPAR
                     FSAQQALNRCTIEGARVLHLEQEIGSLEVGKRADLLIISLNSFAQTPVVNVVDTLVFA
                     SGAQHVRDVMADGKWLVRNRELLGHDADKIMAEARNQAKRLRQKAGLA"
     gene            complement(4612..>4908)
                     /locus_tag="PK710_19565"
     CDS             complement(4612..>4908)
                     /locus_tag="PK710_19565"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOT11983.1"
                     /translation="NVENASFGLCICAERVAVDTMVASGEREIRAIAVVTGAPSPTPP
                     CGMCRQVLAEFAQDIPIHLASTVPGTMARTTSLAKLLPDAFRGDMLPSHEGGAS"
BASE COUNT         1180 a         1388 c         1389 g          951 t
ORIGIN      
        1 gttggatcgt cggctgtcgc tcgaggcggg acgaacggaa gcgctgttcg tttcccatga
       61 atccacgatg ccgcgtgagc gtctttcttc ggataaccac gcgatgacgt cggtacgagc
      121 gtctgagact ccgggccgta cggtttccac ggtggcatgg acggcgttgg gagctggggc
      181 ggctcttgga atcggcggct atttcgcgtt tgccaaggcg gatgacgtgg cgaaggattc
      241 tgccttcgtg tgtgcccaca gcctacgatg cgaccctgat cgggtcgatg tggttcgccg
      301 atgggacgct gcggcgcttg ggatgtgggt tggatcggcg gttggcgtcg gggtaggcct
      361 cggcttgatc ctcgcggggc aaccgaatca gtcatcgacc gcgtgggtgg ttacgcccac
      421 ccaactacaa gcaaggactg tgttttgatg ccgcgacgaa tagctttcgc agcccttgtt
      481 tccgtctccg tgggatgctc cttcgacata tttcatgaca cctcttggcc gaacgcttgc
      541 gatttggatc cttcgttgtc tggctgctcc gatggggggc ctggcgatgg ggacaccgaa
      601 gatcgagctg aacaagatga ggctcccttg acttcggaag gcggcgatgg cgattcgaat
      661 ggctgaaagc tgctttgcac ggaaataacg gaacaattct ccgtttcttc cctcaaaatg
      721 gcccagactc ctggtgttct ttgacagcac tcgccctgga tcgatattct tggggcccca
      781 ctgggttggc aacctttttg gtgaggttgc accagacggt tcaggagccc tgaatggcca
      841 cgaaggttct cgtatttgag agtgacggtg cgttcgccaa cgaattgacc acggagttcc
      901 aagcgcttgg atgcgaagtg gatgtcgtgg acgacggaaa catcggcttg caaatggctg
      961 cgagccatcg tccggacctc atcctgctat ccattgagtt accgcgcacc aacggctttt
     1021 ccgtgtgtaa caggatcaag aaagatccaa gtctgaaaga catcccgctc atcatcatgt
     1081 cgagcatgtc caacaaagac accttcgaac aacatcgtcg gctccgaacc cgagcggaag
     1141 actaccttca caagccgatc gctttcgcgg atttgctaca gcgagttcag cagttcctcg
     1201 aaatctctcc ttccgccggc cccagttccc tcgaagacgc catcatcatc gatgatgata
     1261 tttcgtttga agaaaacgat gatgaggatc tgcacacggt aatgatgaac cgttcctcct
     1321 tggaagcctt cgaaaccgtc gaacccaaag ctcccgcgac aacggctgtt tccaccacgc
     1381 cgtcgcgacc tcgtccatcg tcacgagccc caagccagat ggaggttgat gacttggacg
     1441 tcgatgagtt tgccgatgca gcgtttgacc ggctcctcga tgcaccggaa ccgtcggacc
     1501 aaaaaccatc gcagactgcc ctgcagaccc catcgcaaat cgcagcggat acgatgtcca
     1561 ccgaagtccc tcccatgctc ttgcaagaag gcatcgaaac acgaccagcc acgattcccg
     1621 cgttacccga catccattcc gaagagttgg aacgcttgcg caatgaactc gagcgcatgc
     1681 aaaccaaagc ggtccggctc gaacaagacc tcagcgcttc gcgagctgac aaccgtcgtt
     1741 tggaagaaca ggtcagatcc accgaacaag catcggcgga agtggagcgt cttcaacgcc
     1801 agattgacga actcaaatca cgagcttcca ttcctccggg ccggacaggc acggtatcca
     1861 gtcgcgagtt tctcgatctt cgtgaagctc tcaacaacaa agacaaagag attctcgccc
     1921 ttcgcgacca gatgtcgcag aaggaaaaag aactgctcgc cgccagggac gtcagcttgt
     1981 ccctcgaacg agaaaaagct gatacccaag acaaactctt ggaattcgaa aaacaagccc
     2041 tcgactttcg aatgtccatc gatcgtctca cggtccaaca cggcgaagaa cagcaaaaat
     2101 ccgctgactt acaggcacga ctcaacgcgt cccttcaaca gatcgaagag caacataccc
     2161 gcatcacaga gcaagaagcg ctacgtttgc gagagtccga agaacacgcc gcgacactag
     2221 agacacaaaa acgagaggcc aaccaggccc tcgacaacct ccgaggggaa cgggaaaccc
     2281 tttctacccg catcaccgaa ctcgaaaaag accttagcga ccggaacaac cgaatcaccg
     2341 gactcgaaaa cgatatcaag gcccgagact cgcgcatcac cgaactcgaa ggaaacgtcg
     2401 gtgaccgaga caatcggatc gtaggactcg aaaacgatat caagacccga gactcgcgca
     2461 tcaccgaact cgaaggaatc gtcggtgacc gagacaatcg gatcgtagga ctcgaaaacg
     2521 atatcaagac ccgggactcg cgcatcaccg aactcgaagg aatcgtcggt gaccgggaca
     2581 accgaatcac gggactcgaa aacgacatca agacccggga ctcccgtatc tccgaactcg
     2641 aaggaaacgt cggcgaccgg gacaaccgga tcgcgggact cgaaagcgac atcaaaaccc
     2701 gggactcccg tatctccgaa ctcgaaggaa acgtcagcga ccgaggtcgt cgcatcgatg
     2761 aactcgatgc ttcggttcgc gatcatcagt cgcgcctcga acaacgagac gcgcaaatca
     2821 aggaattgca agagcaggtt cgagatcgag aagcgaagct ggccgatggg gaatcccggg
     2881 ccaaggaaaa ggatgctcgg atcgatggat tggagcagga tcttcgagca gcccgtcagc
     2941 ggacggaaga agtggaagcc gagagaaatg agcgagattc acgcattcgt gatttggaat
     3001 cgagtcttgc caagatcatt acggataggg atgccctggc ggaagcgaag cgagacttga
     3061 gtgaaaagcg ttcggagttg gaggttcagc tcggtgcctc cgtcgaaaag gtcggccagt
     3121 tggaggcgtt ggttttggcg gaacaagagc gagcgaagaa gctgcaagtg aagtgggatc
     3181 acgacaaagc ctcgctcgat cgggcgaaga acgcgttggc ggcggtgttg ttacaaatcg
     3241 aagaagtcga ggcgcgagga ttggaaggct gaccgaaccg cttttacgcc agaccagctt
     3301 tttgacgaag ccgtttggcc tggtttcgag cctcagccat gatcttgtct gcatcgtgcc
     3361 cgagtagttc ccgatttcga accaaccatt ttccgtccgc catgacgtca cgcacgtgtt
     3421 gagcccctga ggcgaataca agggtatcga cgacgttcac cacgggagtt tgcgcgaagg
     3481 agtttagaga tataatcaat aaatccgcac gcttacctac ttccagggaa ccgatttctt
     3541 gttcgagatg gagcacgcgg gccccttcga tcgtgcatcg attcagggct tgttgagcgg
     3601 aaaagcgagc aggtcctttg cggagtttgg cgagcagtcc agccagtctc atttccatcc
     3661 acgcatcgag gttgttgttg caagcagcgc catcggctcc gattccgacg gcaacgccgg
     3721 cttgcatcag ttcatcgagg ggggcaattc ccgatccaag cttgagattg gcgcttgggc
     3781 aatgcaccac gcgcgtacct tgggcggcaa cggaccgcac ctggcgagcg gtgagttgca
     3841 ccccatgagc caacacgcac cgcggccctt ttgcgcccca gcggctcaac acatcgacat
     3901 catcagcgcc gaaagcgcga cgaacggctt ttttttcttc gccatgttcc gcggcatgca
     3961 catgaagaaa agagccggct tgcatgcgtt cgaccgtctc gcggatgaga cgttcgctac
     4021 aagacagaat gaagcgaggt gcataggcat aacgaattcg accatggttt gcgccatccc
     4081 aatgagcgca taacgcatcg ctctgtgcca agcttgcctt ggtgctttct cgcaaggctc
     4141 cgggaacacc tcgaccgcgg tccatcatga cttttccccc aaagacccga attccgaaac
     4201 gaaggcatgc atcgaggatg acatcgtagc catgggtcga acccatatcg agaatcgtcg
     4261 tcgttccccc aagcacgagt tccgcgaggg atagttcagc gctcgcgtgc aggctgcgtt
     4321 catcgtgggc agcttcgaga ggccagacac gtttctggag ccacgtgagc aacggaaggt
     4381 cctcccccat tccccgcatc agcgtttggc atacatgaac gtgagtttgg atgaggcccg
     4441 ggatgatggc gcatccgctg gcatcgacga tttcgacgga agcgtgagca cgccgacgcg
     4501 cgtcaggtcc tatcgcaaga atgcgcccgt tacggatgac gatatcaccg cgaacgacgg
     4561 tttcttgggg atcgcaagca acgatatggc cgtcggaaac gatcaaatca gtcaggatgc
     4621 acctccttcg tgggaaggga gcatgtcacc acggaaagca tcgggcagca gtttcgccaa
     4681 agatgtcgta cgagccatgg tgccgggaac ggtggaggcc aagtggatgg gaatatcttg
     4741 ggcaaactcc gcgagtacct gacggcacat gccgcacggg ggggtgggcg aaggggctcc
     4801 cgtgacaacg gcgatggcgc gaatttctct ttctccgctt gccaccatgg tatcgacagc
     4861 cactcgttcc gcgcaaatac acaggccaaa cgacgcgttt tccacgtt
//
LOCUS       DAOSOR010000458         4899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_17380_length_4899_cov_4.675268, whole genome shotgun sequence.
ACCESSION   DAOSOR010000458 DAOSOR010000000
VERSION     DAOSOR010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17380_length_4899_cov_4.675268"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="PK710_19570"
     CDS             complement(<1..888)
                     /locus_tag="PK710_19570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOT11984.1"
                     /translation="MRRRFRAAIGSMVFALSSVTQAEPGVVLHEYIPPDPKEDIELAA
                     TTTDGRLPAAMMTRSGLVEAPDTSRLPTAAEKAYGMSAPRSVESRYNPDRDTRRPVVS
                     RYDDPFSPATAPYKRLRAFDGVAADYTLTVVDTTLRAVVRGDAVNPGDDAFYGDMTVD
                     LVPGEPVRIPSVGPGARIIRQTTQPSVDVELVRDGAENWFVRGPIRTRLRLVLQLAIA
                     QDTFGGAFGDMAVGGQRRIPAVPSNVARSVHEVVKALGLEGETSTRAIVTYLVTYFRG
                     FEPSEQAPRGRDNIYLDLAL"
     gene            complement(965..2053)
                     /locus_tag="PK710_19575"
     CDS             complement(965..2053)
                     /locus_tag="PK710_19575"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT11985.1"
                     /translation="MGAEFSYSFQEGQWLEGTPYRVVRPLGAGGMGEVYEVDHTRTGT
                     RRAVKVARKHADAPMSRRLLREARALRLIEHPNVVRVYEAGMLSGERPYFSMELLEGM
                     SLRTVIAEQAPMMHERAVRLMLQVLDGLSAIQARHLVHRDIKPSNLFLDHRGVVKVLD
                     LGIVKSIQPTLSQSLTKEGMVLGTTKYMAPEQLMGVSVDARADVFAAGLVLAELLIGR
                     KGRKEIWRRCESEDGFGDAPPGLVSAIRRAVARDPTLRFSSAQQMATALRVWVPAPPI
                     PMPRQKTRVAFCLSTACKPPDPRREPSCRSLRIVHHAGWLWGIQIGILALALTAMVLA
                     TTVAWKSTRQPSLDTSGPAACSVLLERP"
     gene            complement(2162..3112)
                     /locus_tag="PK710_19580"
     CDS             complement(2162..3112)
                     /locus_tag="PK710_19580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11986.1"
                     /translation="MRFLPRFRNVVLFWAGVGAALVGGHVQAQERDSEVATDLFVQGR
                     EALKAGDLSQACHLFADSLRLDPAVGTALNLAECEERRGRFVQALRGWKRAVELAQAT
                     HDEREQVAHQRHDALLHRMPHLTVVLSSDAPGGTTVYRQGELVRADSFGRSIPLDMGE
                     QIIVIRAPGHEDRVMRVMLAEGDKKMLTVGPGPAHRPVSPPVSASSVAQDSSSSRTLA
                     YVLGGIGVSGLAAAAVTGVMLLDSRKTVDEHCDEANRCDSQGMDAAGRGRTLVPINLA
                     AWAVGVVGVGAGTYLFLTAPSKPDVASGNAGAWIQVRGTF"
     gene            complement(3115..4521)
                     /locus_tag="PK710_19585"
     CDS             complement(3115..4521)
                     /locus_tag="PK710_19585"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT11987.1"
                     /translation="MLDSLLGVGGMAAVYAATHRNGNRVAVKMLHTALSMEADLRKRF
                     LREGYVANAVNHPGVVRVLDDDTDEDGAIFLVMDLVVGASAETIAEWRGGRVDPEVVV
                     GISYQLLDIVAAAHVNGVIHRDIKPDNICLTCDGTVKLLDFGVARMRDTATATREGTT
                     IGTPAFMAPEQALGKVKEIDVLSDIYAVGAVMFTMLSGRFVHEGESANELLIMAATRR
                     ARSLADVVSDVPKPIVDVVDRALVFEKALRWPSAAAMRDALEQAHAEAYGTPPVVGAE
                     LASVLDGIESVDLALAGEGDDWPTLLVDPEAELEELHPIDELERAIESNETGKWTPST
                     ASKISTGVGTTSDVEPRATKVRTIRKGLWFGIATAAMVLLIVVFGWVYGNRGAVGMAR
                     PALGGVGVPASRIASAALAPALLSAPGPSSSASGNPVDVYALPSVPGTRVSGGQTAVS
                     GASASPAKSADYDPFAHQ"
BASE COUNT         1104 a         1730 c         1162 g          903 t
ORIGIN      
        1 tagcgccaag tccagataga tattgtcacg tcctcggggc gcttgttcgg aaggctcgaa
       61 cccacggaag tacgtgacca aataggtgac gatcgcacgc gtcgatgtct ccccttcaag
      121 tcccaacgct ttcaccactt cgtggacgga acgagccaca ttggatggca cggcggggat
      181 gcgtctttgc cccccgactg ccatgtctcc aaatgcgccc ccgaaggtgt cctgagcaat
      241 agcaagctga agcaccaaac gcagtcgtgt acgaatcggc cctcgcacga accaattctc
      301 ggctccatcg cgaacgagtt ccacatcgac ggaaggctgt gtcgtttgac gaatgatccg
      361 cgctcctggt cccacggacg gaatacgcac cggttcacca ggcactagat ctaccgtcat
      421 atcgccataa aacgcgtcat cgccggggtt cacggcatcg cccctgacta ccgcccgaag
      481 cgtggtgtcc accaccgtca gcgtataatc cgccgctact ccgtcgaaag cgcgcaatcg
      541 tttgtacggc gccgttgccg gtgaaaacgg atcgtcgtaa cggctcacca cgggtcgtcg
      601 agtatcccga tcgggattat aacgactctc cacgctgcgc ggggcgctca tgccatacgc
      661 cttctccgcg gcagtgggta accgcgaggt atccggagct tcgacaagac cactgcgtgt
      721 catcatcgcc gctggcaggc gaccatccgt cgtggtagcc gcaagctcga tgtcctcctt
      781 cggatcgggg ggaatgtact cgtgcaatac gacacccggc tctgcttgtg tcactgatga
      841 gagcgcgaac accatcgagc caatggcagc gcggaatcgc cgtctcatcg aaaacaccat
      901 gagtctcaat cctaatccct ctcacgaaga agggaaggtt ttgcggtgca agagcccgtt
      961 cgtctcacgg gcgttcgagc aaaaccgagc aagccgcggg acccgaggtg tccaacgacg
     1021 gctgacgagt ggacttccaa gccacggtgg tggccaacac catcgccgtc aacgcaaggg
     1081 caagtatccc aatttgtata ccccataacc atcctgcatg atgcacaatg cgaagcgaac
     1141 ggcaactcgg ctcacgtcgt gggtcaggcg gtttgcacgc cgtcgaaagg cagaaagcca
     1201 cacgtgtttt ttgccgcggc atgggaatcg gcggagccgg aacccacacg cgcaaggcgg
     1261 tggccatctg ttgtgctgag gaaaaacgca gggttggatc acgcgcaacc gctcgtctaa
     1321 tcgccgatac gagtccaggt ggagcatccc cgaacccgtc ttcggattca cagcgcctcc
     1381 atatttcctt tctgcctttc cgaccgatga gaagttccgc caacacgagg cccgccgcaa
     1441 agacgtccgc ccgcgcgtct accgacactc ccatgagttg ctccggcgcc atgtatttcg
     1501 tcgttcccaa caccatcccc tctttggtca aagactgact cagcgtcggt tggatcgact
     1561 tgacgatccc caagtcgagc accttcacca caccacgatg gtccaaaaac aggttgctcg
     1621 gcttgatatc tcgatgcacg agatgccttg cttgaatggc ggacagccca tccaacactt
     1681 gcaacatcag acgaaccgca cgctcatgca tcatcggagc ttgctcggca atgacagtcc
     1741 gaaggctcat gccctccagt agctccatcg aaaaataggg acgctctccc gataacatcc
     1801 ccgcttcata cacacgcacc acgttcggat gctcgatgag ccgaagcgca cgggcttcgc
     1861 gaagcaaccg tctcgacatt ggcgcatcag cgtgttttcg cgcaactttt accgcacgac
     1921 gcgtgcccgt acgggtatgg tcgacctcgt agacttcccc cataccgccc gcgcccagcg
     1981 gccgaacgac acgataagga gtcccttcca accattgccc ttcttgaaaa ctatacgaaa
     2041 actctgctcc cacctcgacc tcgatggccc tcacaccaca atgggcactg ccgcaagttc
     2101 ggccaacgtg gcaacgtggt tgcgtacaat cgtcccctcg gaaagcagga aaacgaaccg
     2161 tctaaaacgt tccccgcacc tggatccacg ctcccgcgtt gccagatgcc acatccggtt
     2221 tggaaggcgc cgtcaaaaac aaatacgttc cagccccgac accaaccacc cctaccgccc
     2281 aagccgcaag attgatcgga acgagcgttc gaccacgccc cgccgcgtcc atcccctgcg
     2341 aatcacagcg attcgcctca tcacaatgct cgtccaccgt cttgcgactg tcgagcaaca
     2401 tcacacccgt caccgccgcg gcagccaatc cactcacccc aatcccccca agaacataag
     2461 ccagcgtccg tgaagacgaa gagtcctgcg caaccgacga cgccgacaca ggcggtgaca
     2521 caggccggtg ggcaggcccc ggccccaccg tcagcatctt cttatccccc tccgccagca
     2581 tcaccctcat gacccggtct tcatgccccg gcgcgcgaat cacgatgatt tgctcgccca
     2641 tgtcgagagg aatggaccgt ccaaaagaat ccgcgcggac gagttccccc tggcgatata
     2701 cggtcgttcc cccaggagca tcggaagaca acaccaccgt aagatgtggc attcgatgca
     2761 aaagcgcatc gtgacgctga tgcgcgactt gctctcgctc gtcgtgggtc gcctgcgcta
     2821 actcaacggc tcgcttccaa ccgcgaagcg cctgaacaaa acgcccgcga cgttcctcgc
     2881 attccgccaa gttcaaggcc gtgcctaccg cggggtcgag tcgcaaacta tcggcaaata
     2941 gatgacaagc ctggctgaga tccccagcct tgagcgcttc tcgaccttgc acgaacagat
     3001 cggtcgcaac ctccgagtcc cgttcttgcg cttgcacgtg tccacccacc aaagccgcac
     3061 ccacccccgc ccaaaacagc acgacatttc gaaatcgggg caggaaacgc atggtcattg
     3121 atgcgcaaac gggtcgtaat cagcgctttt tgcaggcgat gcactcgcgc ccgacacagc
     3181 cgtttgccct cccgaaacac gtgttcccgg cacgctcggc aacgcataca cgtcgaccgg
     3241 attgccgctc gccgatgatg atggccccgg agccgatagt agcgcgggag ccaatgccgc
     3301 cgacgctatc cggctggctg gaaccccaac tccacccaac gccggacgtg ccattcccac
     3361 agcccctcga tttccataca cccacccaaa tacgacaatc agcagaacca tcgcggcagt
     3421 ggcgatccca aaccacaacc ccttgcggat ggtccgtacc ttggttgccc gcggctcgac
     3481 atccgaggtc gttcccactc cggtggaaat cttcgaagcc gtgctcggcg tccacttgcc
     3541 cgtctcgttg ctctcaatcg cgcgctccaa ctcgtcgatc ggatgaagct cctccaactc
     3601 cgcttctgga tccactagca atgtcggcca atcatccccc tcgccggcca gcgccaggtc
     3661 caccgactcg attccatcga gcacgctcgc cagctccgcc cccaccaccg gcggagttcc
     3721 ataagcctcc gcatgcgctt gctcgagcgc atcccgcatc gctgccgccg aaggccaccg
     3781 caaagccttc tcaaacacca aggcccggtc cactacatcc acgatcggct tcggcacatc
     3841 actcaccaca tccgccaagg aacgtgcgcg acgtgtcgcc gccatgatca acaattcgtt
     3901 ggcactctcg ccctcatgca cgaaacgccc agagagcatc gtgaacatca ccgcaccaac
     3961 cgcataaata tccgatagga cgtcgatctc cttgaccttg ccaagcgctt gctccggagc
     4021 catgaaagca ggcgtcccaa tcgtcgttcc ctcccgggtg gccgtcgccg tatcacgcat
     4081 gcgcgccaca ccaaaatcca acagctttac cgtcccatca cacgtaagac aaatgttgtc
     4141 gggcttgata tcgcgatgaa tcaccccgtt tacgtgagct gccgccacaa tatccaacaa
     4201 ttgataggaa atccctacca ccacctctgg atccactcgc cccccacgcc actccgctat
     4261 cgtctccgca ctcgccccaa ccaccaaatc catcacaaga aatatcgcac catcctcgtc
     4321 cgtgtcgtca tccaacacgc gaaccacccc cggatgattc accgcattcg cgacataccc
     4381 ctcccgcaaa aagcgtttgc gcaaatccgc ttccatcgac aacgccgtgt gcaacatctt
     4441 caccgctaca cgattgccgt tgcgatgcgt cgccgcatac accgccgcca tcccgcctac
     4501 gccaagaagc gagtccagca tatacttgcc acagagcact gagccaacac gtgcttcagc
     4561 gcgctggact acatcttcgt gaatttctcc cattcctgcg cgtccagacg acgtgaatta
     4621 ccacgttcgc cctatctcgc atatcacatc cggggtagtc cacccccagt caagcacctc
     4681 gcctaaccac agtgaatgca gccatcacac atactgttct cgtatttcga aacattcgtt
     4741 tccgatgttg acacaaaacc actagcgact cataccccat cggcgagaca aaccaacacc
     4801 atttcttcat tcccaagcca tttgacgcaa cgcgttatga ccgttcgaat cgaacctcgt
     4861 tgacgatacg agccctcacg gtgatgctcc acgctccac
//
LOCUS       DAOSOR010000459         4891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_17409_length_4891_cov_4.357527, whole genome shotgun sequence.
ACCESSION   DAOSOR010000459 DAOSOR010000000
VERSION     DAOSOR010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17409_length_4891_cov_4.357527"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..795
                     /locus_tag="PK710_19590"
     CDS             76..795
                     /locus_tag="PK710_19590"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT11988.1"
                     /translation="MRKGNQPLSPWYGSANTGRIIIKIYGICATASPMKDNGSLAGED
                     GTTSPLSRLFLTRRSVRKYEKGAASQQQVAYVEHCARSFASAMGFRSARLHVVGQGEQ
                     RDAIVRAAVSGVIGKVNPWLPFSHARHLILCGAVLPDDASRNHVQRAIKEASMVMQVA
                     LLAATEQGLGTCWMAGIHHDRVESTFPMPDGARLIAMSPLGLPPARLTLSWDTLSHYL
                     VSKRRKPLEQLWMHERWGVKG"
     gene            792..1268
                     /locus_tag="PK710_19595"
     CDS             792..1268
                     /locus_tag="PK710_19595"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT11989.1"
                     /translation="MNGLLSTLVTELCAENTTKNTAPVAVSHQQLTALLSCARVAPSA
                     DNSQTWRFVTVWSPSTRKALSQLVIPSIAPFVAEASVVIVACGVPALISHVRREQPFV
                     MIDVPIAITHIVLRATEMQIPYVWTLDCDETAMRAQLGIPAHARVIGLIALGRDSE"
     gene            complement(1260..3707)
                     /locus_tag="PK710_19600"
     CDS             complement(1260..3707)
                     /locus_tag="PK710_19600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOT11990.1"
                     /translation="MATSERNLSPRGKMRGFFWRWCKRAITSLLVLLALGSVGFFFAV
                     RHYEADLPSTAEIKEGRYHPPQVTRVLARDGTVLSELFTERRTVVPIESLPSHVKLAI
                     LAAEDAHFYEHEGLNYLGMVRAMLINLRSGRTRQGASTITQQVVKNILLDPERTYRRK
                     IREALLARRIEQELTKDQILELYLNQIYFGHGRYGIEEASRYYFGRHARDLSLSQAAL
                     LAGIVAGPEAYSPRKNPSKSEQRRQYVLGQMQTKGFINADQQQAALHEPIVLATAQSE
                     NAELAPEVIEIVKRTLQQVAGPGAVLGGYTVTTTIDPKLQQEARRSLRANLEKYDERH
                     GLQGPLAPPKKMPKKGSRDAPFEGMPRFEDHKILVGEVTKANDELGLIYVRVGDLEGV
                     VRLEDHARYNPKKLVASKFAPNGTLLRVSLLAKETDPKEIEGKNTEAPPVPLRLESGP
                     QSALVALDIRTREVLALVGNYEAVVGGLDRATQARRQPASTFKPIVYSYALHTRRFTP
                     ATLVDVRPNSVRGIRAADEAEAASTETIRLREALAKSVNVAAVHVIREVGPANVVSWA
                     KALGITTPMGPDLSLPLGAYEAKPIEMVNVYATFAAGGQFELPRLITRIQDPNGKEVP
                     LPPTPPSRRVMEESEAYLITSMLTSVIDRGTGQLARGVGRPVAGKTGTSNEAKDAWFI
                     GYSTDIAVAVWTGYDDARPLGRREAGATAALPAWVSFMKAAHAGTPPTDFPRPADVLV
                     VSIDPTTGLLPAEDQTDTLTEFFLSGTEPTERVELDAGVHDALASEQEVPEVPAMERD
                     AETLPALPPPEDTIPLF"
     gene            complement(3836..>4891)
                     /locus_tag="PK710_19605"
     CDS             complement(3836..>4891)
                     /locus_tag="PK710_19605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT11991.1"
                     /translation="LAPLFGMASAPPLPPLAPEPPVGGSGAKGGSGGAEADGGSTGGS
                     GAYAGSGGAAGSAGEAGASGAAGQAGGATGGSGGETGGTGGATGGTGGATGGTGGSTG
                     GTGGTGGSTGGTGGATGGTGGSTGGTGGSTGGTGGSTGGTGGATGGTGGTGGATTYSH
                     TITIDGTNDFAAEETFETTTSSFQGFIAWDANNLYVGLSGSDVGQASPTVFFVVYLGG
                     SGGTTTGVTYQSQTPQLPFEAKWQVHWKANNSNTRAVQWNGSAWVDDSWDFTGKIYQK
                     GSFVEMAIPLSRIGSPSTLAVAMAMLNESSSGEWTWAGVPKGMFVDGKDPDFTKYFNF
                     ALAGSTPPNAHSILPAP"
BASE COUNT          929 a         1452 c         1483 g         1027 t
ORIGIN      
        1 cgggtcacac caccgaaacc gttccccaaa acggcctttc tgggatggga acgaactagg
       61 aactaggaac taggaatgcg aaaggggaac cagccccttt ccccttggta tggttcagcc
      121 aacacggggc gaattatcat caaaatctac gggatttgtg ctaccgcgtc ccccatgaag
      181 gataatggca gtctggctgg cgaagacggc accacctcgc ccctctcccg cctctttctc
      241 acgcgaagaa gcgtacgaaa atacgaaaaa ggcgcggctt cccagcagca agtcgcctat
      301 gtggagcact gcgctcgcag tttcgcctct gccatgggct ttcgctcggc acgattgcac
      361 gtggtagggc aaggagagca acgggacgcc atcgtccggg ccgccgtatc gggggtcatc
      421 ggtaaagtga atccctggct gcccttttcc cacgcacgcc acctcatcct ttgcggtgcc
      481 gtgttgcccg acgacgcttc ccgcaaccac gtgcagcggg ccatcaaaga agcgtcgatg
      541 gtcatgcaag tcgctttgct tgccgccacc gagcaaggcc tcggcacgtg ttggatggcg
      601 ggcatccacc acgaccgcgt ggaatccacg ttcccgatgc ccgatggggc tcgcttgatc
      661 gccatgtcgc cgctgggctt gcctcccgct cgcttgaccc tttcctggga taccctatcc
      721 cattacctcg tatccaagcg tcgtaagccc ctcgaacaac tctggatgca tgaacgatgg
      781 ggagttaaag gatgaacggc ctgctttcca cgttggtgac cgagctttgc gcggaaaata
      841 ccacgaaaaa tacggcgccg gtggcggttt cccatcagca gctcacagcc cttctgtcgt
      901 gcgccagagt cgctccttct gctgataatt cgcagacttg gcgctttgtc accgtttggt
      961 ccccctcgac ccgcaaggcc ctcagccagc tcgtcatccc atccatcgcc ccgtttgtcg
     1021 ccgaggcgtc ggtggtcatc gtggcttgcg gtgttcccgc actcatctcc catgtgcgac
     1081 gggaacagcc tttcgtgatg atcgacgtcc ccattgccat cacccatatc gtgctaaggg
     1141 cgacggagat gcagatacct tatgtgtgga cgctggattg cgacgagacc gccatgcgcg
     1201 cgcagctcgg gattcctgcc catgcgcggg tgattggcct gattgccctg ggccgggatt
     1261 cagaatagcg ggatcgtatc ttccggggga ggtagagcgg gcagtgtttc cgcatctcgt
     1321 tccatggcgg gcacttcggg cacttcctgt tcggaggcaa gcgcgtcatg cacacctgca
     1381 tcaagctcga cccgttccgt gggctccgtg cccgacaaga aaaactccgt cagcgtatcg
     1441 gtctggtctt cggctggcag caagcccgtg gtcgggtcga tggaaaccac cagcacgtca
     1501 gccggtcgag gaaaatccgt gggaggggtc cccgcgtggg ccgctttcat gaacgacacc
     1561 caggccggta gcgccgccgt tgcgcctgct tcacgacgcc ccaggggccg cgcatcgtcg
     1621 tagcccgtcc aaaccgctac cgcgatatcc gtggaataac cgatgaacca ggcgtctttt
     1681 gcctcattgc tcgtgccggt ctttcccgcc acggggcgac caacgccacg ggccaactgc
     1741 cctgttcccc ggtcaatcac tgacgtgagc atgctggtga tgaggtaggc ttcgctttct
     1801 tccatgacgc gacgggaagg aggagttgga gggaggggaa cctccttgcc gttggggtcc
     1861 tggatccggg tgatgagcct ggggagttcg aattggccgc ctgcagcaaa ggtcgcgtaa
     1921 acattgacca tttcgatggg tttggcttcg taggcaccga gtggaaggga taagtcgggg
     1981 cccatggggg tggtgatccc aagcgctttt gcccaggata cgacattggc agggccgact
     2041 tcgcgaatca cgtggacggc agccacattg acggattttg caagcgcttc gcgcaaacgg
     2101 atcgtctcgg tggacgcggc ttcggcctca tcggcggcgc gaattccccg tacggaattg
     2161 ggtcgcacat cgaccaaggt ggcgggggtg aagcgacgtg tgtgcaacgc ataagaatag
     2221 acgatgggtt tgaaagtcga tgcgggttgc cttcgggctt gggtagcccg gtcgaggccg
     2281 ccgacgacag cttcataatt acccacgaga gcgagcacct cgcgggttcg gatatcgaga
     2341 gcgacaaggg cggattgggg accggattcg agacgaagag ggacgggagg ggcctcggtg
     2401 tttttgcctt cgatttcttt ggggtcggtt tctttggcaa gcaagctgac gcgaagcagc
     2461 gtgccattgg gggcgaactt gctcgccaca agctttttgg ggttgtagcg agcgtggtct
     2521 tcgagccgca ccacaccttc gaggtctccc acgcgaacgt agatcaagcc gagttcgtcg
     2581 ttggctttcg tgacttctcc gacgaggatc ttatggtctt cgaaccgcgg cattccttcg
     2641 aagggcgcgt cgcgagagcc ttttttcggc atttttttgg gaggagccag ggggccttgc
     2701 agcccgtgcc gttcgtcata tttctccaaa ttggctcgaa gggagcgacg agcttcttgt
     2761 tgtaatttgg ggtcgatggt ggtggtgacg gtatatccgc cgagcacggc gccggggcca
     2821 gcgacttgtt gcagggtgcg tttgacgatt tcgatcactt cgggcgcgag ttcggcgttt
     2881 tcgctttggg cggtagccag cacgatgggt tcatgaagtg cggcttgttg ttgatcggca
     2941 ttgatgaagc ctttggtttg catttggccg aggacgtact gccgacgttg ctcggatttc
     3001 gatgggtttt ttcgcggaga ataggcttcc ggtccggcga caatcccggc gagcagcgcg
     3061 gcttgcgaca gggagaggtc gcgcgcatgg cgcccgaagt agtatcgcga cgcctcctcg
     3121 ataccgtagc gcccatgacc aaaataaatc tgattgaggt atagttcgag gatctgatct
     3181 ttggtcaatt cctgctcgat tcgtcgcgcg agcaaagctt cgcgaatttt tcgacgatag
     3241 gtccgctccg gatcgagcaa gatatttttc accacctgtt gtgtaatcgt gcttgcgccc
     3301 tggcgcgttc gaccagagcg caggttgatg agcatggccc tcaccatgcc caggtagttc
     3361 aagccttcat gctcataaaa atgagcgtct tccgctgcaa gaatcgcgag cttcacgtgc
     3421 gaaggcaacg attcgatcgg tacgacggtg cgtcgttcgg tgaatagctc cgatagcacg
     3481 gtgccatcgc gcgccagcac acgcgtgacc tgcggggggt ggtatcgccc ttctttgatt
     3541 tccgcggtgc tcggaagatc cgcttcgtaa tgccgtacgg cgaagaaaaa tccaacggat
     3601 cccaaagcca agagcaccaa cagcgaggtg atggctcgtt tgcaccagcg ccagaaaaaa
     3661 ccgcgcattt ttcctcgagg ggataggttg cgttccgatg tggccatgga ttcgccagag
     3721 ctagcattca gagacaaatt ggccaacgcc cgagcggtgg agatttgttc ccgcaggatg
     3781 tcgaacgcta ggcaaaagcg tcatcggggg gcgaacttgg gtagaaggtt cgagatcagg
     3841 gcgcgggcag gatagaatgc gcgttcggag gtgtcgaacc agccagggca aaattgaaat
     3901 acttagtaaa atcagggtct ttcccgtcga cgaacatgcc tttgggcacc ccagcccatg
     3961 tccattctcc cgatgagctt tcattcagca tcgccatggc aacggccagg gtggaggggg
     4021 aaccgatgcg ggaaagagga atcgccattt ccacaaatga gcccttttgg tagatcttgc
     4081 ctgtaaaatc ccagctatca tccacccagg cggacccatt ccactgtacg gcccgcgtgt
     4141 tggaattatt cgctttccaa tggacctgcc atttggcttc gaagggcaat tggggggttt
     4201 gcgactggta ggttacgccc gtggtcgtgc cgcccgagcc gccgagatac accacgaaaa
     4261 aaaccgtggg agaggcttgt cccacatcgg aaccgctcaa tccgacgtag agattattgg
     4321 catcccacgc aatgaacccc tgaaacgagg aggtggtcgt ctcgaaagtt tcttcggcgg
     4381 caaaatcatt ggtgccgtcg atcgtaatag tgtgggaata ggtggttgcg ccgcctgttc
     4441 cgccggtgcc gccggtagct ccgcccgtac cacccgtgct tccgccggtg ccacctgtac
     4501 ttccgccagt accgcccgta cttccgccgg tgccgcctgt ggcgccgccc gtaccacccg
     4561 tacttccgcc agtgccgccc gtaccgcccg tacttccgcc cgtaccaccc gtagcgccgc
     4621 cggtaccgcc cgtagcgccg ccggtgccgc ccgtttcacc gcctgagccg cctgtcgcac
     4681 cccctgcttg cccggcagca ccggatgcac ctgcctcgcc agcgcttccc gcagcgccgc
     4741 ccgaacccgc gtaagctccc gaacctccag tggaaccgcc gtcggcttcg gcgcctccgc
     4801 taccgccctt ggcacctgaa ccgcccacgg gcggttcagg tgccaagggc ggtagcggag
     4861 gcgccgaagc catgccaaac aagggagcca a
//
LOCUS       DAOSOR010000460         4883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_17438_length_4883_cov_5.081400, whole genome shotgun sequence.
ACCESSION   DAOSOR010000460 DAOSOR010000000
VERSION     DAOSOR010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17438_length_4883_cov_5.081400"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="PK710_19610"
     CDS             <1..1245
                     /locus_tag="PK710_19610"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT11992.1"
                     /translation="GVTFGCPVGSSVIGSATSSQICLQDPTVSRVHAEISVQHDRVVL
                     RDCESTNGTFIEEVRIRDADVREGNRIRVGSTVMRVERSGEMLIAELSANDSFGEMVG
                     RSPAMRAVFSVLERVAPTDATVLIQGETGTGKDLAARAIHENSRRAEGSFVAVDCGAI
                     AETLFESELFGHVRGAFSGAISDRTGVIEEADGGTLFLDEIGELPLGLQRKLLRVLEN
                     REIRRVGSNRSKRVDVRLIAATNQPLSAAVNAGYFREDLFFRLAVVMVELPPLRQRRG
                     DIPILARHLLARLSDQPVSLPAEILASLGSRGWPGNVRELRNFLERGLSLGWAGERIP
                     ADPKPISTALDDLVPTHMPLKEARVAWMEQFDEVYLRALLRKTGGNVSAAAREAGVGR
                     RFLQRTMKRIGWSDKTQSGKED"
     gene            1309..2679
                     /locus_tag="PK710_19615"
     CDS             1309..2679
                     /locus_tag="PK710_19615"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT11993.1"
                     /translation="MAIPSNQPSNDEPDPIGMIVDGKYDICQRVAAGGVGTVYRARHR
                     FTGRQVALKLLNEQYTRDKIMSERFLREAKAAAEIGHPNIVEVVDAGVDEAVGVYVAF
                     DFLEGMDLADAYRRKLLSIRQILDVGDGVLDALAAAHQAGIIHRDIKPPNIFLLPGEH
                     GSFSVRVLDFGAAKRQKVSVEESLTTFGTVIGTPAYMSPEQASGQSVDPRTDIWSVGA
                     VLFRGLAERPPFAERNPSLLLACIIRDTAPSLAEFRPDLPMELISVIDKSLARDPAHR
                     WPSAQAMREALMQARASVSGAVATMTPEQLSASYVRRVEPNLVESPSIEKTIQAVPSS
                     ILALQRKQQRKRMAGALAVAVFLVVILAFLWFRPAMQREQPSASAATATISQPTVPVA
                     SNSAAPTMGSASATSSAASVSPIPTRIHIPTVVVPGVVAPPVKGSRALPTGSQRPPVV
                     PIREYE"
     gene            2676..3584
                     /locus_tag="PK710_19620"
     CDS             2676..3584
                     /locus_tag="PK710_19620"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOT11994.1"
                     /translation="MKIRTVLLTMALSLGSSVAFADDAQLARSHFERGVALLDDSQFA
                     QALVELEKSRSIRETAPVLFNLGLAYRGVGRYLEALDCFRQFLEVRDHAKHKKMAEMA
                     TTLIDELQAGLIHVKVRVSGGATSVRIDGKPTGQRDGVHSAVLDPGTHVFEAVREGYE
                     PAVRRIEFKAGEHAEVILDAKSHPKSSRISIEASPAQAEIWLDGKLVGRGHYHGQIPL
                     GTRRLRIVAPGHEPAERTVNVKPGSRQQVTIALTQSSRPITKQWWFWATGAALVAGAV
                     VTAVLVQPESKTPYEGSLGFVSEALR"
     gene            complement(3542..>4883)
                     /locus_tag="PK710_19625"
     CDS             complement(3542..>4883)
                     /locus_tag="PK710_19625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HOT11995.1"
                     /translation="TVEGCTFIGHDNGQQTVAISTKAPTWGWVIRGNIVSGAGTGLYL
                     GNSNGESAFVAGIIENNLIEDTVGYNMQIKHQKPRPSIAGMPTGPNSTIIRHNVFIKN
                     DRPSPDGDRPNLLVGGFPDTGPGSEDLYEIYGNVLIGNHRESLLQASGRVSIHDNVFI
                     DVTDTAILLRNHDLPLKLARVYNNTIFSAKRGINVGSNAAQGSAIFGNLIFADTGVSG
                     GSDVRDNLIDTVANAAQYVKNPSTKLGNMDFYPLTGKCEGAAIDMAVAKSDIDYDVDF
                     NGTSKAGFTFRGAYAGSGTNPGWPISNTIKDTEGSTPDPDAGTGGSGGANTGGSGGAN
                     TGGSGGANTGGSAGANEGGSAGTGTGGSSGSSAGNAGNTNTGGKSGSGGSSAGASGKP
                     ANPSAAGEDDDSGCSCTTARSTGNSAWLALLTLGLAALGRRHRSASDTNPSDPS"
BASE COUNT          950 a         1304 c         1533 g         1096 t
ORIGIN      
        1 ggggtaacgt ttggctgtcc ggtggggtcg agtgtgattg gcagtgcgac gtcgagtcag
       61 atttgtctgc aagatccgac ggtttcgcgg gttcatgcgg aaatctcggt gcagcacgat
      121 cgggtggtgc ttcgggattg tgaaagcacc aacggtacgt tcatcgaaga agtacggatt
      181 cgcgatgcgg atgttcgtga gggcaatcgg attcgcgtgg gttcgacggt gatgcgggtg
      241 gagcggtcgg gggagatgct gattgcggag ctttcggcga acgacagttt tggagagatg
      301 gtggggcgga gtccagcgat gcgcgcggta ttttccgtgc tcgagcgggt cgctccgacc
      361 gacgccacgg tgttgattca gggggagaca ggaactggaa aggacttggc ggcaagagcc
      421 atccacgaaa actcccgtcg agcggagggt tcgttcgtgg cggtcgattg cggggcgatt
      481 gccgagacgc tttttgaaag cgagttattc gggcatgtgc ggggagcgtt cagcggtgcg
      541 atttcggacc gtacgggagt catcgaagag gcggatgggg gaacgctatt tctcgatgag
      601 atcggggaac tgccgctggg gttgcaacga aagctgttgc gggtcctcga aaaccgagag
      661 atacgacgtg tcggttcgaa ccgctccaag cgtgtggacg tccgtctcat cgcggcgacc
      721 aaccagcccc tctctgccgc ggtgaacgct ggatactttc gagaagactt atttttccgc
      781 cttgccgtgg tgatggtcga gcttcctccc ttgcggcagc gtcgtggtga tattcccatt
      841 ctcgcacgtc atttgttggc gcgtctgtcg gatcaacccg tatcgcttcc tgcggaaatc
      901 cttgcatcat tgggttcgcg gggctggcct ggcaacgtcc gcgagctacg caattttttg
      961 gagcgaggtc tgtccctggg ctgggcagga gagcgtatcc ccgcggatcc aaagccgatt
     1021 tcaaccgccc tggacgatct cgttcccacg catatgcctt tgaaggaggc ccgggtggcg
     1081 tggatggaac aattcgatga agtgtatctt cgcgcacttc ttcgaaagac agggggaaat
     1141 gtctccgcgg ccgcaaggga ggctggcgtg ggtcgacgtt ttcttcagcg gaccatgaag
     1201 cgaatcggct ggtcagacaa gacacaatct ggaaaagaag actgatcgct tcctccgcaa
     1261 ttccgctcgc acattggaat gccagccaca tccgtgtaat ctatccctgt ggcaattccc
     1321 tccaaccaac cgtccaacga tgagcctgat ccgatcggga tgattgtcga cggcaagtac
     1381 gacatttgcc agcgtgttgc cgcgggggga gtggggacgg tatatcgtgc gcgccatcga
     1441 tttacggggc gccaagtagc tctgaagctt ctcaacgaac agtatacccg cgataaaatc
     1501 atgtcggaac ggttcctccg cgaagcgaaa gcagcagcgg aaataggcca tccgaatatc
     1561 gtcgaagtcg ttgatgccgg tgtagacgag gccgtggggg tgtacgttgc cttcgatttc
     1621 ctcgaaggaa tggatctagc cgacgcctat cgtcgcaaat tgctttcgat ccgccagata
     1681 ctcgatgtgg gagacggggt cctcgacgcc ttggcggcgg cccaccaggc gggaatcatc
     1741 catcgagata tcaagcctcc caatattttt ctccttccgg gagaacacgg cagcttttct
     1801 gttcgcgtgt tggattttgg tgccgcgaag cgccagaaag tcagtgtgga ggaatcgctg
     1861 acgacctttg gaacggtcat tggaacgccg gcgtatatga gtccggagca agcctctggg
     1921 cagtcggtgg acccacgaac ggatatttgg tccgtggggg ccgtgctatt tcggggtctg
     1981 gcggagcgtc ctccgtttgc ggagcgaaat ccgagtctac tattggcctg tatcatccgg
     2041 gatacggctc cgagccttgc ggagtttcga ccggatttgc cgatggagtt gatttcggtg
     2101 atcgacaagt cgctggcccg ggacccagcc catcgatggc cgagcgcaca ggcgatgcgc
     2161 gaagccttga tgcaagctcg tgccagcgtc tcaggggccg tggctaccat gacccccgag
     2221 cagctttcag cctcctacgt acgtcgagtc gaaccaaacc tcgtcgaatc cccatcgata
     2281 gaaaaaacga ttcaggccgt cccctcgtcg atcctcgctc ttcagcgaaa acagcaaagg
     2341 aaaaggatgg cgggagcatt ggccgtcgcg gtttttctcg ttgtcatcct cgcatttctg
     2401 tggtttcgac ctgccatgca gcgcgaacag ccatcggcat cggcagcgac agccactatt
     2461 tcccagccta cggtacccgt cgcgtcaaac tccgcagctc ccacgatggg atcagctagt
     2521 gcaacgtcga gtgcggcttc ggtttctccc attcccacga ggatacatat tcctaccgtg
     2581 gttgttcctg gcgtggttgc gccacctgtc aaagggagcc gagcgttgcc gacgggttcg
     2641 cagcgcccac ctgtcgtacc gattcgagaa tacgaatgaa gattcgaacc gttctgctca
     2701 caatggcctt gagcctgggt tcttcggttg cctttgcgga tgatgcccag cttgcacgct
     2761 ctcacttcga gcgaggggtt gctttgctcg atgattcgca gttcgcgcaa gccctcgtgg
     2821 agctagaaaa atcgcgttcg attcgagaga cggcacctgt gcttttcaac cttgggttgg
     2881 cttatcgtgg ggtgggccgg tacctcgaag cgctcgattg tttcaggcaa ttcctcgagg
     2941 ttcgggatca tgcgaaacat aagaagatgg cggagatggc caccacattg atcgatgagt
     3001 tgcaagcggg gttgattcac gtgaaggtgc gtgtatcggg cggggcgaca tctgtgcgta
     3061 tcgatggcaa gccgacaggc caacgagacg gggttcattc cgcggtgctg gatccgggaa
     3121 ctcatgtttt tgaagcggtt cgtgaaggtt atgaaccagc ggtgcggcgc atcgagttca
     3181 aggcggggga gcatgctgaa gtgattctcg atgcgaaaag ccatcccaag tcatcgagga
     3241 tttcgatcga ggcttctcca gcgcaggccg agatatggtt ggacggcaag ctcgtgggtc
     3301 gtgggcatta tcacgggcaa ataccgctgg gtacgaggcg tttgcggatc gtggcgcctg
     3361 ggcatgagcc tgcggaaagg acggtaaacg tgaagccggg ttctcgccaa caagtcacca
     3421 ttgcgctcac ccaaagcagt cgtccgatca cgaagcagtg gtggttttgg gccacggggg
     3481 cagcgttggt tgcgggagcg gtggttacgg cggtgctcgt gcagccggag agcaaaacgc
     3541 cttacgaggg atcgcttgga tttgtgtcgg aagcgctgcg gtgacgcctc ccaagggcag
     3601 caaggccgag ggttagaagt gcgagccaag ccgagttgcc cgtggaacga gccgttgtgc
     3661 aagagcaacc gctgtcgtcg tcttcccctg cggcggaggg attagcgggt tttccagagg
     3721 cgcccgcaga actaccgccc gagccagatt ttccaccggt gttggtgttg cccgcgttac
     3781 cagcgctgga tccgctgctt ccccccgtgc ctgttcctgc cgaaccgcct tcgtttgccc
     3841 ccgcgcttcc cccagtattc gcccctccgc tgccgccggt attcgcccct ccgctgccgc
     3901 cggtattcgc ccctccgctg ccgccggtac ccgcgtccgg gtcaggcgtg gagccttccg
     3961 tatctttgat ggtattgctg atgggccaac cggggttggt tcccgagcca gcgtaggcac
     4021 ctcgaaaggt gaagcccgct ttggaagtgc cgttgaaatc cacgtcataa tcgatatcgc
     4081 ttttggcgac ggccatatcg atggcagcgc cttcgcactt gcccgtcaaa ggataaaaat
     4141 ccatgttgcc gagtttggtg gatgggtttt ttacgtattg agcggcgttt gcgacggtgt
     4201 cgatcaagtt gtcgcgaaca tcgctaccac ccgacactcc ggtgtcggca aaaatgagat
     4261 tgccaaaaat cgcagaacct tgagcagcgt tgctacccac gttgattcct cgcttcgcac
     4321 taaaaatcgt gttattgtag acgcgtgcga gcttgagagg caggtcatgg ttccgcagca
     4381 agatggcggt gtcagtcaca tcgatgaata cgttgtcgtg aatcgaaacg cgcccagaag
     4441 cctggagcaa tgattcgcgg tgattgccaa tgagcacgtt accgtaaatt tcgtaaaggt
     4501 cctccgaccc tggacctgta tctgggaagc cacccaccaa taagttggga cggtccccgt
     4561 cggggctcgg gcgatcgttt ttgatgaaaa cgttgtgtcg aatgatggtg gagttgggac
     4621 ccgtgggcat gccagcgatg gaaggacgcg gcttttgatg cttgatttgc atgttgtagc
     4681 cgaccgtatc ctcgatgagg ttgttttcga tgatgccggc cacaaacgcg gattcgccgt
     4741 tggaattgcc gaggtatagc ccggtgcccg cacccgaaac gatatttccg cgaatgaccc
     4801 atccccaggt cggcgctttc gtggaaatcg ccaccgtctg ttgcccgttg tcatgaccga
     4861 tgaaggtgca tccttctacg gtg
//
LOCUS       DAOSOR010000461         4883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_17439_length_4883_cov_4.744408, whole genome shotgun sequence.
ACCESSION   DAOSOR010000461 DAOSOR010000000
VERSION     DAOSOR010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17439_length_4883_cov_4.744408"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="PK710_19630"
     CDS             <1..1050
                     /locus_tag="PK710_19630"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT11996.1"
                     /translation="YHLEEDLARRDFTINAIAYDPIDRLLTDPSGGCADLQHRVIRAV
                     GVANDRFFEDGLRILRAARFAATLHFDIEPDTARAMKECASRLCCISQERIRDEWLKT
                     LGAEQPSRGLRIMLSTGIIEATLPELLPMIGCTQNRYHAFDVWEHTLQTLDACRAEPI
                     LRLAMLLHDIGKPEIRGINEKTGDYTFYHHEVSGARIAEAIVQRLRLSNEQQRRVVHL
                     VRHHLIPYDPSCTDSAVRRWLRRVGTEHLQDILEMARADARGKGVDASETLETLEHLR
                     QRVEELLRQSAALSVRDLSINGHVLMGELGLPPGRQIGQILEHLLQLVIDDPSRNERH
                     LLLAEARTYLQSQPN"
     gene            1171..1443
                     /locus_tag="PK710_19635"
     CDS             1171..1443
                     /locus_tag="PK710_19635"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOT11997.1"
                     /translation="MSEPFTREAIESNLIEVFSKYKHGNVEVQRTSVITADLGVDSLA
                     VMEIVAELEDTFDLTFPDEDLPNVKTVADVADMIVRGLKESGRLHS"
     gene            1440..3236
                     /locus_tag="PK710_19640"
     CDS             1440..3236
                     /locus_tag="PK710_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HOT11998.1"
                     /translation="MTVRSLAEAVERTAISSPARGYRFIRDDKFESDFFSFSQLEERT
                     RELAGCLQSLGLKKADRLALILPDSDQFVLMFLAALRAGVIPVPIYPPTGLGQLSGYV
                     DNTRHIVRKSGATCLMTTSLIKPLLGGVQSACPDLAHITTFAAVMEAKAPFKPVDIKA
                     DDIAFLQFTSGSTNRPKGVILTHANLLANSRNILEEGLASDPQTDSGFTWLPLFHDMG
                     LIGFVLAPIVYGIEVSFMSPLLFLKRPSSWLRGMSRFRATITFGPNFAYALAVKRVRS
                     KEIEGIDLSRWRVAGCGAEPIRAETLEAFADHYAPYGFRKEAFYPAYGLAESTLAVSF
                     SRSVPTDRVKASVLWSTDEAVACEASDPDALRIVNCGKAFDGHQVQVFALDDTRCEHP
                     LADRKVGEMRLKGPSVSPGYFEEPELTRASFVDGWFLTGDLGYLADGNVHICGRSKEV
                     IIIHGRNFYPQDIEWEASQVEGVRKGNVIAFGTRGDGGDREAVVLAYESAITDAAEIE
                     RITKEIRGRIQEVIGVMLDDIIALAPGVLPKTSSGKLQRTKTRDLFERGELTRRRSQR
                     ETNRVDQVKEIAKSQLGYLKLAVFGGRKSPKN"
     gene            complement(3291..>4883)
                     /locus_tag="PK710_19645"
     CDS             complement(3291..>4883)
                     /locus_tag="PK710_19645"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HOT11999.1"
                     /translation="LSAPVEVKPQNCPASNFGESIAIDGDTMVIGAPGVSADQTEGVA
                     YVYVRAGSTWVEQAQLAVPSLPLNTRFGHAVAVSGNTVVVGAPDSSNDRSGSAHVFVY
                     QYGGWSLQESLEDGAPTASDRFGNAVSIQGDRLLVGAPGSTWPGFPSGKAVLFTRKSS
                     KWKVEKTFSDPPGDLSLNFGYSVALAAPWVFIGSPGGSFMGKQKVVYYESTSSGWKDC
                     EVLEPSKLKANSRFGSSISVSGSLLAVGAPEENDSVPEAGAVFVFHWNGTKWEELDKL
                     KVTPPVIHGAFGKNLSVSGHRIIAGMAPADKAWLYAVRGDLGTPCGEDDACASGHCVD
                     GVCCDGPCGGGVADDCEACLKALGATKDGICTALQDTSCHDDDPCTDTAICVDGTCQR
                     STSKSDGAPCPSGTCQGGTCVDTQVDAGDEDSGDSGMAGQAGVDSGGKSGSGGMGGGG
                     TGDGAAGIEETGGGGTGGAQGRDGGTGGSPRPSSSPQEEVSSFYACGVRPRPMCGLYG
                     WLSVLLWVTAASVRRSRRPVAR"
BASE COUNT         1100 a         1521 c         1253 g         1009 t
ORIGIN      
        1 tatcatctcg aagaggatct cgcccgacgt gacttcacca tcaacgcgat tgcctatgac
       61 cctatcgacc gcctgcttac ggacccgtcg gggggatgcg cggacctcca gcatcgggtc
      121 attcgcgccg tgggagtcgc taacgatcgg tttttcgaag atgggttgcg tatccttcgc
      181 gccgcccgat tcgccgctac cctgcatttc gatatcgagc cggatacagc acgagccatg
      241 aaggaatgcg cgtcgcggct ttgctgcata agccaagaac gcattcgtga cgaatggctc
      301 aaaaccctcg gtgcggagca accctctcga ggtctgcgca tcatgctatc gacagggatt
      361 atcgaagcca ccttgcctga actcctgccc atgatcggat gcacgcaaaa ccgctaccac
      421 gctttcgacg tctgggagca tacgcttcag acgctcgacg cctgtcgcgc agaaccgatc
      481 ttgagactcg ccatgctatt gcatgatatc ggaaaaccag aaattcgtgg aataaatgaa
      541 aaaactggcg attacacctt ctaccaccac gaggtttcag gtgctcgaat cgctgaagcc
      601 atcgtgcagc ggttgcggct ttccaacgaa cagcagcgtc gcgtggtgca tctggtgcgt
      661 catcatttga ttccttacga cccatcttgc accgactcgg ccgtgcgacg atggctgcgg
      721 cgtgtgggaa ccgagcattt acaagatatt ttggaaatgg cccgggcgga tgctcgggga
      781 aagggagtgg atgcgagcga gacactagag acactggagc atcttcgaca gcgggtcgaa
      841 gagctactgc ggcaaagcgc ggccttgagc gtgcgagact tatccatcaa cggtcacgtg
      901 ctcatgggcg aattgggtct tcccccgggc cggcaaattg ggcaaatctt ggagcatcta
      961 ttgcaactgg ttatcgacga cccgtcgcga aacgagcggc acctgctttt ggcagaggct
     1021 cggacctatc tccaatcgca gcccaattga acgaaaaacc tgtcattcat gggtttgtgg
     1081 aatcttccag gagcagttgt tgcgttcgct ttgacctacg acgcaaaccc gactacccag
     1141 agcccatcgt aggaataacg agggattccc atgtccgaac cgttcacacg agaagcaatc
     1201 gagtcgaacc tcatcgaggt cttcagtaag tacaagcacg gcaacgtcga agtccaacgc
     1261 accagtgtca tcaccgcaga cctcggcgta gactccttgg ccgtcatgga aatcgtcgct
     1321 gaactcgaag atactttcga tttgacgttc ccagacgagg acctgcccaa cgtcaagacc
     1381 gtggctgacg ttgcggatat gatcgtgcgc gggctgaaag aatccgggag gctgcattca
     1441 tgacagtgcg aagcctagcg gaagctgtcg agcgaacagc tatttcctcg cccgcacgcg
     1501 ggtatcgttt cattcgtgat gacaaattcg agagtgattt tttttcattc tcgcaactcg
     1561 aagagcgaac ccgcgaactc gcgggttgtt tgcagtccct tggattgaaa aaagccgacc
     1621 ggctggctct cattctgcct gattccgacc agttcgtgct catgttccta gcagccctac
     1681 gagcgggtgt gattcccgta cccatctatc cgccgacagg actcggccaa ttgtccggtt
     1741 atgtcgacaa tacacgtcat atcgttcgaa aaagtggcgc aacgtgtctg atgacgactt
     1801 cccttatcaa gcctctgcta gggggcgtgc aatcggcgtg tccggacctc gctcacataa
     1861 cgacattcgc tgccgtcatg gaagccaaag ctcccttcaa gcctgtcgat atcaaggccg
     1921 atgacatcgc atttttgcag tttacgagcg gctccacgaa ccgtcccaaa ggggtgatcc
     1981 tcacccatgc caacttgctc gctaacagtc gaaacatctt ggaagaaggg ctcgcctcgg
     2041 atccgcagac cgattcgggc ttcacatggc ttcccctctt ccatgacatg ggcctgatcg
     2101 gattcgtcct ggctcccatc gtctacggca tcgaggtgtc gttcatgtca cctctgctgt
     2161 ttttgaaacg cccctcgagt tggttgcgag gcatgtctcg attccgcgcc accatcacct
     2221 tcggaccaaa ctttgcgtac gccttggccg tcaaacgtgt tcgctccaaa gaaatcgaag
     2281 gaatcgacct gtctcgctgg cgcgtggcag gctgcggcgc agaacccatc cgcgctgaaa
     2341 ccctcgaagc cttcgcggac cactacgccc cttatggctt ccgcaaagaa gcattctatc
     2401 ccgcctatgg cctcgcggaa tcaaccctcg ccgtgtcctt ttcacgctcc gttcccaccg
     2461 accgagtcaa agcctccgtc ctttggtcca ccgatgaagc cgtcgcttgt gaagcttctg
     2521 accccgatgc cttgcgaatc gtgaactgcg gaaaagcgtt cgatggacat caagtgcaag
     2581 ttttcgctct cgacgacacg cgctgtgagc acccccttgc agaccgaaaa gtcggtgaaa
     2641 tgcgactcaa aggaccaagt gtctcgccgg ggtacttcga agaacccgaa ctcacccgcg
     2701 cttctttcgt cgatggttgg ttcttgactg gcgacctcgg gtatctcgcc gatggcaacg
     2761 tccacatctg tggccgttcc aaagaagtga tcatcatcca cggacgaaac ttctacccac
     2821 aagacatcga atgggaagcg agccaagtcg aaggcgttcg caaaggcaat gtcatcgcct
     2881 tcggaacccg cggcgatggc ggagaccggg aagccgtcgt gctcgcgtac gaatccgcca
     2941 tcaccgatgc cgcggaaatc gaacgcatca ccaaggaaat ccgcggacgt attcaagagg
     3001 tcatcggggt gatgctcgac gatatcattg cgctggctcc tggtgtcctt cccaagacat
     3061 cgagcggaaa actgcaacgt acgaaaacga gagacttgtt cgagcgtgga gaactcaccc
     3121 gaagacgaag tcagcgcgaa accaatcgtg tcgaccaagt caaagagatc gcaaaaagcc
     3181 agctcggata cctcaaactc gctgtcttcg gcggtcgaaa atcgccgaaa aactagcaca
     3241 aacggatcct caccgctttc gcccttttct catcgacgat tgaaaccgcg ttatcgtgcg
     3301 accggtctac gcgatcgacg aacggacgcc gcggtcaccc atagcaagac cgaaagccat
     3361 ccatacagcc cacacatggg ccggggccgc acgccgcaag cataaaacga actgacttcc
     3421 tcctgtggcg atgatgaagg gcggggcgat ccaccggtgc cgccatcgcg tccctgcgcg
     3481 ccacccgttc ctccaccacc ggtttcctcg atgccagcgg cgccatcgcc ggttccccca
     3541 ccacccattc ctccactacc gcttttcccg ccgctatcca cccccgcttg ccctgccata
     3601 ccgctatcgc cactgtcttc atctcccgcg tccacctgtg tgtcgacaca cgtgccccct
     3661 tggcacgtcc ccgaaggaca cggagcgccg tccgatttcg aagtgctgcg ctggcaggtg
     3721 ccgtcaacac atatcgccgt atccgtacag ggatcgtcat cgtggcaact cgtatcttga
     3781 agggcggtgc aaatcccgtc cttcgtggcc cccaacgcct tgagacaagc ttcacaatcg
     3841 tcggccactc ctccgccgca aggcccgtca caacataccc catccacgca atggcccgac
     3901 gcacatgcgt catcttctcc acagggagtc cccaggtccc cgcgaaccgc atacaaccag
     3961 gccttatccg ctggtgccat gccagcgatg atcctatgtc cggatacgga taggttcttg
     4021 ccgaatgcgc cgtggatgac aggcggcgta accttgagct tatccaactc ctcccacttg
     4081 gttccattcc aatgaaatac gaatacagct ccagcctcgg ggaccgaatc gttctcttcc
     4141 ggagctccga ccgccaacaa ggatccagat acggatattg aagatccaaa tctggagttg
     4201 gctttcagtt tcgaaggttc gagcacctca cagtccttcc agccggaaga tgtcgattcg
     4261 taatacacga ctttctgctt gcccataaac gagccgcctg gcgaacctat gaaaacccac
     4321 ggtgctgcca acgcgacgga atacccaaaa ttgagggata gatccccggg agggtccgaa
     4381 aaggtctttt ccactttcca tttcgaactt tttcgagtga aaagaactgc cttgccgctg
     4441 ggaaaacccg gccaagtgga gccgggggcc ccaacgagca aacgatctcc ttggatactc
     4501 accgcatttc cgaatcgatc actcgcagtg ggagcaccat cttcgaggga ctcctgtagc
     4561 gaccagccac cgtattgata cacgaaaacg tgggccgaac ccgaacggtc gttcgacgaa
     4621 tccggagccc ccacgacaac cgtatttccg gacaccgcta ccgcatgccc gaacctcgta
     4681 ttgaggggaa gggaagggac cgccaattgc gcttgctcga cccacgtcga acccgcacgc
     4741 acatacacat acgccacacc ttcggtctga tcagcactaa ccccgggtgc ccctatcacc
     4801 atcgtgtcac cgtcgatcgc aatggattcc ccgaagttgc tggcggggca attctggggt
     4861 tttacctcga caggggcgga taa
//
LOCUS       DAOSOR010000462         4864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_17514_length_4864_cov_4.583697, whole genome shotgun sequence.
ACCESSION   DAOSOR010000462 DAOSOR010000000
VERSION     DAOSOR010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17514_length_4864_cov_4.583697"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PK710_19650"
     CDS             complement(<1..521)
                     /locus_tag="PK710_19650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12000.1"
                     /translation="MPRDERDSFRNPWRVSPIARPILESMATHASPAYRDAAELAARC
                     LHAGTLVGIHESNHRFLITLRQWQARPAHAPSGALFQCLARRSPPLSGNDLPGWIVLQ
                     NGFRGGRLIMVHELSHFANRFLVWRLGHAPGFSRNEAMVRSRWLDEVAARHLAYLAES
                     AIDPEASHPAWPAP"
     gene            810..1805
                     /locus_tag="PK710_19655"
     CDS             810..1805
                     /locus_tag="PK710_19655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOT12001.1"
                     /translation="MPFEAIFGQAAAVGTLEQMLRAGRVHHALRFEGIEGVGKEMAAM
                     AFAQALVCTAGNPTGCGGCSACERAVTMGEGVPSVPLHPDVIFVERGLYPPEVLGRTR
                     PEAQELSVDQIRTVVLERAAFPPHEGKARIYIVRRAEELSISAANALLKTLEEPGRNT
                     YFVLLVSRPARLLSTIRSRTLRVRFAPLGDDVLRKILASHGISSEEVERVIPLCAGSA
                     TAALELADAHASEQREAFVRQALEALQSKDSAAALAFADSQGRDKAVVRLHLGALAVH
                     FARQAREAVLRQELSARQFALRHELVLQAISDVERNASPVLAIENMMLRLRAETG"
     gene            1810..3477
                     /gene="ricT"
                     /locus_tag="PK710_19660"
     CDS             1810..3477
                     /gene="ricT"
                     /locus_tag="PK710_19660"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOT12002.1"
                     /translation="MTQGPNEKNPAGSGVEERPKPKRRRRGRRSSKERAPQGAVAPEQ
                     PGTMPFREPKEGRDPKEGREPKEGREEGRSRSRRRRGKGRKPEPSGKEALVREPEKPA
                     KKELERPLGPPAASGRLAGAERSFPTSLPRHDAAALPPAADGQEAKGRDRRNRRAKRE
                     EVPSKKGRLARPERNKLEQGPIRWSQAPAPDLPAERPVSVLPSAPRPRELDHPVFLSA
                     AEPLIDPELPVDIKPWEALIADEPEDGILYNTVVVRGHRSQRSRLYDAADNVYERGES
                     VAIEADGGSPGTGVVVLPNRRMLYRGRLPRVVRRLGASDMTGESRAKRREAEVFRIAR
                     HAASNLGLPIKIIRAQAEPSGKIVVVFACEERIDFRDLYRRISAAAQGRIELRQIGVR
                     DAAKILGGIAPCGLQLCCASFLRDFAPVSIKMAKEQGLVLNPQRVSGLCGRLLCCLNY
                     EDALYRTQRKLLPKLGKRVVTPSGPGKVRDVDVLSQTIRVTLDNGEVITVKPSDVTAM
                     FPSSSEERVGSRGEHRFGPATQAVHDGVESVFEGRDSSDGAEPVDET"
     gene            complement(3511..4140)
                     /locus_tag="PK710_19665"
     CDS             complement(3511..4140)
                     /locus_tag="PK710_19665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12003.1"
                     /translation="MSALILVVVGIVAGALIVGVLATALLWSWHRRKRALPAPTPVPL
                     LNAPAPRFGIGGFSLGDVVLFDQTETWLTAALRIQDAGNEEATLFFSERSADNDVLLL
                     RLRPELTTFRVHPISLPGAGVGPATLEYDQEIFTRVRLIPVMIERHGKTAPDVGSTGA
                     WSWFESASGDIIGSLHTDHATLTFHGHRTQAGEMMHLAAEGATFRDEEF"
     gene            4313..>4864
                     /locus_tag="PK710_19670"
     CDS             4313..>4864
                     /locus_tag="PK710_19670"
                     /inference="COORDINATES: protein motif:HMM:NF019950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1726 domain-containing protein"
                     /protein_id="HOT12004.1"
                     /translation="MGERDSAYRRCVVLRGSREQTSREARQLLRNLADVLWVDSSRAF
                     DFSVQRWLGRSLQAVCLDLHDGLDADRLGRCHGLIRAGGALVLRMPPKGITPTTPSLT
                     VYPFTDTEVGTRFWRRLERHLDAHGPEDATASLVPCEPFAEGTREQKRVVEQIVARVG
                     QSRPCCMALLAARGRGKSAALGMA"
BASE COUNT          961 a         1250 c         1687 g          966 t
ORIGIN      
        1 ggggctggcc acgcgggatg gctagcttcc gggtcgatgg cgctttcggc aagatatgcc
       61 agatgacgag cggcgacctc gtcgagccat cgggagcgaa ccatggcttc gttgcgtgaa
      121 aagcctgggg cgtggccgag ccgccacacc aaaaagcggt tggcgaaatg cgataactca
      181 tgcaccatga tcaatcgtcc accccggaag ccgttttgca agacgatcca tcctggaagg
      241 tcgttgccgg acaagggcgg ggagcgtcgc gcaaggcatt gaaataaggc accgctgggg
      301 gcatgggcgg ggcgcgcttg ccattggcgt agtgtgatca aaaagcggtg attcgattcg
      361 tgaataccca ccaatgtccc tgcgtgcaag cagcgggcgg caagttcggc ggcgtcacga
      421 tacgccgggc tagcgtgggt tgccatggac tcgagaatgg gtcgagcgat cgggctgaca
      481 cgccatggat tgcggaaaga gtctcgttcg tcccttggca tgggacaagg gtagggattg
      541 gtaaggcaag gggcaagctt gcgggcaagt gccggaacaa accgagttga taagcgttgc
      601 gggtagtgga tgccggtagc gtcggaaaag gggaagaaag aaggatcttc ttaaatatat
      661 agtaaattca tattgttgcg cgacaaacgg cgccgcttcg acgtcccgag ggtttgagca
      721 aaggcgggag gctggtttga ggtggtaatg tgagaggggc gggctcaacc ccctatcgac
      781 ttgggcgcgg ctgtactagg ttgtggctcg tgcccttcga agccattttc ggtcaagccg
      841 ccgcggtagg aacgctcgag cagatgctgc gagcgggtcg ggttcatcac gcgttgcgtt
      901 tcgagggcat cgagggtgtg ggaaaagaga tggcggcgat ggcgttcgcg caggcgctgg
      961 tctgcacggc gggcaatccc acggggtgcg ggggttgttc cgcgtgtgag cgagccgtga
     1021 cgatggggga gggggttccg tcggttccgc ttcaccccga cgtcatcttt gtcgagcggg
     1081 gattatatcc gccggaagtc ctgggccgga cgcgtcccga ggcgcaggag ttgtccgtgg
     1141 accagattcg tacggtcgtt ctcgaacgcg cggcatttcc gccgcacgag ggaaaagccc
     1201 ggatctatat cgtacgtcgt gcggaggaat tgagtatttc cgccgcgaac gcgctgctca
     1261 aaacgttgga ggagccgggg cgtaatacct acttcgtttt gctggtttcg cgtcctgcga
     1321 ggcttttgtc cacgattcgt tcgaggacgt tacgtgtgcg ttttgcgccg ttgggcgacg
     1381 atgtgctgcg aaagattctg gcgagccatg ggatatcgtc ggaggaggtg gagagggtca
     1441 ttcccttatg tgcgggatct gcgacagcgg cgctcgaact tgcggatgcc cacgcctcgg
     1501 agcagcgaga ggcgttcgta cgtcaggcgt tggaggctct tcaatcgaag gattccgcgg
     1561 cggcgctggc gtttgcggat agtcaagggc gagacaaggc agtggttcgt ttgcatctcg
     1621 gcgcgctggc ggtgcatttt gcgcgacaag ctcgcgaagc ggtcctgcga caagagcttt
     1681 ccgcccggca atttgcgctg cgtcatgaac tggtgttgca agcgatctcc gatgtggaac
     1741 gaaacgcctc gccggttctt gccatcgaaa atatgatgct gcgcttgcgc gcggagacag
     1801 ggtgatatcg tgacccaggg acccaacgag aaaaatccag ccggttccgg tgtcgaggaa
     1861 cgtccaaagc caaaaaggcg acgtcgtggg cgtcgttcgt cgaaagaacg tgcgccgcaa
     1921 ggggcagtgg cgccggaaca gccaggaaca atgcccttcc gggagccgaa agaaggccgt
     1981 gacccgaagg aaggccggga gccgaaagaa ggccgggagg aagggaggag caggtcacgg
     2041 cgacgtcgcg gaaaggggcg aaagcccgaa ccatcgggga aggaagcgct cgtccgggaa
     2101 ccggaaaaac cagcaaaaaa agagctggaa aggccattgg gccctccggc ggcgtcgggg
     2161 cgtttggcgg gagcggaacg atctttccct acctcgttgc ctcgtcacga tgcggccgcg
     2221 ttacctcccg cggcggacgg acaagaagcc aaaggcagag atcgccgcaa ccgtcgtgcg
     2281 aagcgcgaag aggttccgtc gaaaaaaggg cgtctggcgc ggccggagcg gaacaagctg
     2341 gaacaagggc cgattcgttg gagtcaagcg ccagccccgg atctgcctgc ggagcgcccg
     2401 gtttcggttc tgccgtcggc cccgcgtcct cgcgaactcg accatcccgt atttctaagt
     2461 gcggccgagc ccctcatcga cccggaactg cccgtggaca tcaaaccatg ggaggcgctt
     2521 atcgccgacg aaccggagga tggaattctc tacaataccg ttgtggtgcg gggccatcgt
     2581 tcccagcggt ccaggctcta cgacgccgcc gacaatgtat atgagcgcgg ggaatcggtg
     2641 gccatcgaag ccgatggggg aagtccaggc acgggtgtgg tcgttctacc caatcgtcgg
     2701 atgttgtacc gcggtcggtt gccgcgtgtg gttcgtcgtc ttggggcttc ggatatgact
     2761 ggggaatctc gtgccaagcg aagggaggcc gaagtgtttc gcattgcgag acacgcagct
     2821 tccaatcttg gattgccgat caaaatcatc cgagcgcagg ccgaacccag tggaaagatc
     2881 gtggtggttt tcgcttgtga ggagcgtatc gattttcgcg atttgtatcg tcggatatcc
     2941 gccgcggcgc aaggtcgtat cgagctgcgg caaattggcg tacgggatgc cgcgaaaatc
     3001 ctgggcggga tcgctccttg cgggttgcag ctttgttgtg cctcatttct gagggatttc
     3061 gcaccagtca gcatcaagat ggccaaagag cagggcctgg tgctcaaccc tcaacgtgtc
     3121 agtgggctct gcggtcgctt gctctgctgt ttgaattacg aagatgcgtt gtatcgcacc
     3181 cagcgcaaac tccttcccaa gcttggcaaa cgggtagtta ccccttctgg tccgggaaaa
     3241 gtccgcgacg tagacgttct ttcgcaaacg attcgtgtca cgctcgataa cggtgaagtc
     3301 atcacggtca agccttcgga tgtgacggcc atgttcccgt catcgtcgga ggaacgtgtg
     3361 ggctcgcggg gggaacaccg attcgggcct gccacacaag cggtccacga tggcgtcgaa
     3421 tcagttttcg agggtcggga ttcgagcgat ggcgcggaac ccgtggatga aacgtagtcg
     3481 ttccgaatac atgtaactat ttatttttat tcagaattct tcgtctctga acgtggctcc
     3541 ctccgcggcg aggtgcatca tttccccggc ttgcgtgcgg tgaccgtgaa acgtgagggt
     3601 ggcgtggtcc gtatgcaggc ttccgatgat atcgccggaa gcggattcga accaggacca
     3661 ggcgcccgtg gagccgacgt cgggagctgt tttgccatgt cgttcgatca tgacggggat
     3721 gagtcgtacg cgtgtgaaga tttcttggtc atattcgagg gtggcggggc ccacgccggc
     3781 gccgggcagc gagatgggat gcacgcgaaa tgtggtgagt tcggggcgga gtcgcagcaa
     3841 aagaacatcg ttatccgcgc tgcgctcgga aaaaaacagc gtggcctcct cgttgccggc
     3901 gtcttggatg cgaagggcgg cggtcagcca ggtttcggtt tgatcgaaga gaacgacatc
     3961 gccaagggag aatcctccga taccaaatcg gggggccggt gcgttgagca agggcaccgg
     4021 tgtgggggca ggcaaggcgc gttttcgccg atgccatgac cagagcaggg ccgtcgccaa
     4081 caccccaacg atcaaggctc cggccacgat tcccacgacc acgaggatga gagcgctcat
     4141 cggaatgtat cccgggttcg ttgtatcgct atcatgacca tcaggatatc agcaggtcgg
     4201 gcaaacggtg atagagatga ttcgacgaag ggcaaagacg gaggttggcg ggtgggggag
     4261 gcagaaagaa acgatgaggt gaggcgtcat cgaagcgaga cgggagccgt ccatgggtga
     4321 gcgtgattcc gcgtatcggc gctgtgtggt gttgaggggg agtcgggagc aaacttcgcg
     4381 ggaggcgcgg cagcttttgc ggaacctcgc ggatgtgttg tgggtcgact cctcacgcgc
     4441 ctttgatttt tccgttcagc gttggcttgg tcggagtttg caggcggttt gtctcgatct
     4501 tcatgacggc ttggatgcag atcgtttggg gcgctgtcat gggctgattc gcgcgggtgg
     4561 agcactcgtt ttgcgaatgc caccgaaagg catcacgcca acgacccctt cgcttacggt
     4621 atatccgttt acggatacag aggtgggcac acggttttgg cgtcggctcg agcggcatct
     4681 cgacgcccat ggaccggagg acgccactgc gtcgctggtg ccatgcgagc cctttgccga
     4741 ggggacgcgc gaacagaagc gagtggtcga gcaaatcgtt gcgcgcgtgg gacagtcgcg
     4801 tccctgttgt atggcgcttt tggcggctcg tggacgaggc aagtcggcag cgttggggat
     4861 ggcg
//
LOCUS       DAOSOR010000463         4859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_17536_length_4859_cov_3.721690, whole genome shotgun sequence.
ACCESSION   DAOSOR010000463 DAOSOR010000000
VERSION     DAOSOR010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17536_length_4859_cov_3.721690"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PK710_19675"
     CDS             complement(<1..345)
                     /locus_tag="PK710_19675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12005.1"
                     /translation="MNRFLSCIIAIVGGYCGWVGSVGAEEPLSEPGASAVTTDERPAA
                     VSTAPETSVAQAVSTPEQPAADNSASPSAPAAYALPFYLRGVVPGTVARLESTYAMYK
                     LPAPVDESGNTLT"
     gene            676..1269
                     /locus_tag="PK710_19680"
     CDS             676..1269
                     /locus_tag="PK710_19680"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HOT12006.1"
                     /translation="MHDATAIVLAGGRSKRMGINKAFLDFDGKPLIARLVDMLRQAFA
                     EVLLSANTPQPYAFLGVEIVADEPKDAGPLGAIISCLAKSSRELNFVMGCDMPFVRLD
                     LVHSLVSMAAQQHCDAVIPQSSEGLYEPLLAVYKRSALEPARALLARGNRRVLDLAQE
                     IRALHPMIDRQSAININTPEDYAAALAAWRQWHDPAL"
     gene            complement(1404..2642)
                     /locus_tag="PK710_19685"
     CDS             complement(1404..2642)
                     /locus_tag="PK710_19685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12007.1"
                     /translation="MKPAFRFVPIASSLHDTRAITALQAEFSPALERMGGACWEDPSD
                     PWTPVIWFVLTGGTEQRLLDAMADHERRVGPGPVMLLAHGGQNSLPAALETLARLQQE
                     GRKGRVLFVSSPSDAEGFERLAQGIHDLEAWRKMRQSRLGVIGRPSDWLVASTVDPQV
                     VRAGWGPTWVPIEMQQFFAVFDEARRRPPTPSEPYPLGVSQGEVSHEAVRNAGRVALA
                     LETVIKTHRLDAITMRCFDLVMERKTTACHALSRLNDAGITAGCEGDVASVVGMMWVR
                     ALLDEPSWMANPAAIDEYSSHVVLAHCTVPRSMVGDCTLRVHFESGIGVALAGRFARG
                     PVTLLRVGGRQLDKAWFEEGELVATSTEEGLCRTQAKVVLREGAATELLLRPLGNHLL
                     LVRGHHGARLRSWWEQFGPG"
     gene            complement(2692..3039)
                     /locus_tag="PK710_19690"
     CDS             complement(2692..3039)
                     /locus_tag="PK710_19690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12008.1"
                     /translation="MRQRWLQRRTVGKASIRVGVILFTVSVGMTMVACESSPGVVESD
                     EKEERARLVQCCQALAHEAWNSDPPNSNVLHAARDCEALARQGHFEQARTKLRAAGHA
                     TACEGCPPNGCRP"
     gene            3271..4029
                     /locus_tag="PK710_19695"
     CDS             3271..4029
                     /locus_tag="PK710_19695"
                     /inference="COORDINATES: protein motif:HMM:NF012255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HOT12009.1"
                     /translation="MPVIHHATKEIQLKIVYYGPGLGGKTTNLKYLHAASRPDLRGKL
                     LSFTTESDRTYFFDLLPVELGLFREYQIRLHLCTVPGQIALDSTRRLVLRNVDGIVFV
                     VDSQIEALNANIESIRNLETNLRLQGDDPDRMPLVVQYNKRDLPNIIPVEDLSVALKV
                     PNEVKEFEACAKTGMGVSDTLKAIVKDCLRLVADPRRAKEGRTPSILPGIRASMYPDS
                     LPASLSEPPIPAPPKVPLMFEDDLVFSPDDPSKS"
     gene            4229..>4859
                     /locus_tag="PK710_19700"
     CDS             4229..>4859
                     /locus_tag="PK710_19700"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOT12010.1"
                     /translation="MKKVFAILTILVVTLSVALGYRIHQQQAYRQAPSGGSGVIEATQ
                     IDLGTRLSSRIRTIHVREGQPVQKGQPLVEFDCEEPLASVDEAKARLAMARAGVQAAE
                     ASERVARGNTQAAWQTARASAAQVKALQAERNHAEKEAFRLASLHTQGAISDSAFDTT
                     ETRVASVQQQQLALEANTAAAGARADAAYRSQQAAAVQITSAEYAVAAAE"
BASE COUNT         1080 a         1583 c         1257 g          939 t
ORIGIN      
        1 cgtcagcgtg ttgccggact catccacggg agccgggagt ttgtacatgg cataggtgga
       61 ttcgaggcga gcgaccgtac cgggaacgac gccacgcaag taaaaaggca gcgcataggc
      121 agcgggtgcc gacggcgaag cggaattgtc agcggctggt tgttccggcg tggatacggc
      181 ctgcgcgacg cttgtttcgg gtgcggtgga gacggccgcc ggcctttcat cggtggtgac
      241 agccgaagca ccaggctcag acaaaggttc ttcagcaccg acgctgccga cccacccaca
      301 ataaccgccc acaatcgcga tgatgcacga taaaaatcga ttcatggtgt ctcgctccgg
      361 acatgaaccg aggcccttgg agcacgaaac ccagagtgct gtccacgaat atttgatgac
      421 atgacatcga tccatggctt cttctttgcg tgacctcgtc cgccccaagg caacaggcga
      481 agaccattgg ctttctccac tttttcaacc ctcatcgcta tacttcccag gtggtttcga
      541 caccgaggca acgccgcttc cctcccccaa ctctagccgc gatgattccc atcgagggaa
      601 tcgcatgctc cgatcccatg aaaacacttg aaatacatga acttactgcc cttgggccca
      661 gccggaatgc gctcgatgca tgatgcaact gccatcgttc tcgcgggagg acgcagcaaa
      721 cgaatgggca taaacaaggc ttttctcgat ttcgacggca agcctctcat cgcccgtttg
      781 gtggacatgc tgcgtcaagc gttcgccgag gtgctcctaa gcgccaatac gccacagccg
      841 tacgcatttc tgggggtcga gatcgttgcg gacgagccaa aggatgcggg accgcttggg
      901 gccatcatat cgtgtctagc gaaatccagt cgggaactga acttcgtgat gggatgcgat
      961 atgcccttcg ttcgcctcga cctcgtgcat agcctggtat cgatggcggc acagcagcat
     1021 tgcgatgcgg tgattcccca atcctccgaa gggctgtacg aaccgctgct ggcggtctac
     1081 aaacgcagcg ccttggaacc tgcccgagct ttgctggcgc ggggaaatcg ccgcgtgctc
     1141 gaccttgccc aggagattcg cgcgctacat cccatgatcg accggcagtc ggccatcaac
     1201 atcaatacgc ctgaggatta tgcggctgct ttggccgcct ggcgacaatg gcatgatcca
     1261 gcgctttgac cggaagctga ggcaggccca gcgggaagga ataaaaatat tcataaaatc
     1321 aatatgttat gctcccacaa cgcacactta tccctttgag cacgggcttt ggtcccatct
     1381 acgagccagg gccgaagccc cacctacccc ggcccaaatt gctcccacca cgaccggagc
     1441 cgagccccat gatgccctcg caccaacaat agatgattgc ccaaagggcg aagcaacaac
     1501 tccgtcgcgg ctccctcgcg caacaccact ttcgcttgcg ttctacaaag cccttcctcc
     1561 gtgctcgttg ccaccaactc gccttcctcg aaccaggctt tgtcgagctg gcgtcctccc
     1621 acacgcaaca gcgtcacggg gcctctggca aaccgtcccg ccaacgctac gccaatcccg
     1681 gactcgaaat gcacacgaag cgtgcagtct cccaccatcg aacgaggcac cgtgcaatgg
     1741 gcaagcacca catggctcga atactcatca atcgcggcgg gattggccat ccatgaaggc
     1801 tcatcgagca acgctcgaac ccacatcatc ccgaccacgc ttgccacatc cccttcacag
     1861 cccgccgtga tacccgcatc gttgagacgg gaaagcgcat ggcaagccgt tgtttttcgt
     1921 tccatgacca gatcgaaaca gcgcatggtg atcgcatcga gccgatgggt tttgataacg
     1981 gtttcgaggg ccaaggccac cctgccagca tttcgcaccg cttcgtggct cacctcccct
     2041 tgcgaaacac caaggggata cggctcggag ggcgtcggag gccgacgccg cgcttcgtcg
     2101 aataccgcga aaaactgctg catttcgata ggtacccagg tcgggcccca gccagcacga
     2161 acgacctggg gatcgaccgt gctggctacc aaccaatcgg atggacgacc gatgacgcca
     2221 agtcggcttt gtcgcatctt tcgccatgcc tcaagatcgt gaattccttg tgctagccgt
     2281 tcgaaccctt cggcgtcgga aggggaagaa acgaatagta cccttccttt tcggccttcc
     2341 tgctgcaacc gcgcaagcgt ttcgagggca gccggcaagg agttctgacc cccatgggca
     2401 agaagcatca cggggccagg acccacgcga cgctcatggt ctgccatggc atcgagcaag
     2461 cgctgctcgg tcccccctgt caatacgaac cagatcacgg gagtccaagg gtccgaaggg
     2521 tcttcccagc acgctcctcc catccgttcg agagcgggcg aaaactcggc ctgaagcgcg
     2581 gtgatcgcac gggtatcatg caacgacgaa gctatcggaa cgaaccgaaa cgcgggtttc
     2641 atgctcttct ccacgacggc tcatagcata tcgcaccgaa agccacagcg atcaaggtcg
     2701 acaaccgttg gggggacaac cttcgcaagc ggtcgcgtgc ccggccgcgc gaagcttcgt
     2761 cctggcttgc tcgaaatgtc cttgtcgcgc cagcgcctca caatcgcgcg cggcgtgcag
     2821 gacattcgaa ttcggagggt ccgaattcca cgcttcatgg gccaaggcct gacaacactg
     2881 cacaagacgc gctctttctt ctttttcgtc gctttccacg acccccggcg acgattcgca
     2941 tgccaccatg gtcatcccaa cgcttacggt gaataagatc acccccacac gtatggaggc
     3001 cttgcccacg gtccgacgtt gtaaccagcg ttgtctcacg gccacaatta ttctaacctg
     3061 ttatcgaccg ataacgctat cctccggcgg ctcaaccttc gaattcagag ctggatgacg
     3121 aaaaacccga tccgtcaagc caaaggtccc catggaacaa aaccgccgat gaggctttca
     3181 accattcaga tcgccgccaa cccgctgctg gagcagtccc cctattccgt ggtagtctat
     3241 acgtagaact catcactcgc aacagccccc atgcccgtca tccaccacgc caccaaagaa
     3301 atccaactca agatcgtgta ttacgggcct ggtctgggag gcaaaaccac caacctgaag
     3361 tacctgcacg ccgccagccg ccccgatctt cgcggaaaac tcctctcgtt taccaccgaa
     3421 tccgaccgta cctacttttt cgacctgctg cccgtcgaac ttggactctt tcgcgaatac
     3481 caaattcgcc tgcacttatg caccgtccca ggccagatcg ccctggattc cacccgaaga
     3541 ctcgtcctgc gtaacgtcga tggcatcgtc ttcgtcgtgg atagccagat agaagcgctg
     3601 aatgccaata tcgaaagcat ccgcaacctc gagaccaacc tgcgtctgca aggcgatgac
     3661 ccggaccgta tgcccctggt ggttcaatac aacaaaaggg accttcccaa catcatccct
     3721 gtcgaagacc tatccgtcgc cctaaaagtc cccaacgaag tcaaggaatt cgaagcctgc
     3781 gccaaaaccg gcatgggagt cagcgatacc ctcaaagcca tcgtcaaaga ctgcctccgg
     3841 ctcgtggccg acccacggcg cgccaaagaa ggaagaacac catccattct gccaggcatt
     3901 cgagcctcga tgtaccccga ctcccttccc gcatcgttgt ccgaaccgcc gattccagca
     3961 ccccccaaag tgcccctgat gttcgaagat gatcttgtat tctcgcccga cgacccttcg
     4021 aagtcatgat gggctatcga aaaccacaag ggatgtcatg cgattaactg gttgatttta
     4081 ttatttaatt ctacgacccc atcctttttt ccccgaggtg actcccattc cgacagattg
     4141 tcgggctcat gaacacctgg tgccctgtat tgcgcccaat cactgctttt tctgctccgg
     4201 catggcctgt gcatcctacg ccttcaccat gaaaaaggtg ttcgctattc tgacaatcct
     4261 ggtggtgacg ctgtcggttg cgctcggcta ccgtattcat caacaacaag cgtatcggca
     4321 ggctccgtcg ggcggctcgg gtgtgattga agccacgcag attgacctgg gcacccgtct
     4381 ttcatcacga atccgcacca tccacgtgcg cgagggccag cctgttcaaa agggacagcc
     4441 ccttgtcgag ttcgactgtg aggagccttt ggcaagcgtg gatgaggcta aggcgcgatt
     4501 ggccatggcc cgcgcgggag tgcaagccgc cgaagcttcg gagcgggtcg cgcggggcaa
     4561 tacgcaagcg gcatggcaaa cggcgcgagc ctcggccgcc caggtcaaag ccctgcaagc
     4621 cgaacgcaac catgccgaaa aagaggcgtt tcggctcgct tccttgcaca cgcaaggcgc
     4681 tatctccgat tccgccttcg ataccaccga aacacgggtc gcaagcgttc agcagcaaca
     4741 gttggcgctc gaagccaata ccgccgccgc aggcgcccgc gccgatgccg cctatcgctc
     4801 gcaacaagcc gcagccgtgc aaatcacctc ggccgaatat gccgtcgccg ccgccgaag
//
LOCUS       DAOSOR010000464         4850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_17565_length_4850_cov_4.106778, whole genome shotgun sequence.
ACCESSION   DAOSOR010000464 DAOSOR010000000
VERSION     DAOSOR010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17565_length_4850_cov_4.106778"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1399
                     /locus_tag="PK710_19705"
     CDS             <1..1399
                     /locus_tag="PK710_19705"
                     /inference="COORDINATES: protein motif:HMM:NF025943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chondroitinase-B domain-containing protein"
                     /protein_id="HOT12011.1"
                     /translation="GDWYQAPPTPSREFYVSPTGSGDGSSVSSPMSFHAAIQQAKEGD
                     WYWMLEGTYKDFYRIIEDGKASKPIVFRNYANGRARIDGNIQVEGEYNWLWGLEVDNL
                     YGITNDVGDGENIGMFRRGIRIINCIVHDGSSTFGSWNFPEQVIYGNIIYRGYHSIYT
                     QNTYSNGVKYFVHNISMDAFTDSGNGPYEFHAYGEGGDVSGLYLEGNVFANTDLEGKS
                     LIGGKNDTPNDHMTLIGNYFYGSHAQLGYARPVQAVFKDNKLYKASLRWRYFWGDGNE
                     RFPNPPPAVVTGNTFYQPDGGDPHVQFQTSTYKACSDPKGYCREDGTSKIRPDDVWDQ
                     NTYLPNFRADFHANNQNIEGMTSLSQWQNATQSAGKMFDSNSTVTAMPTQPMVAVLPN
                     AYEPGRAHVVIFNFAKASTTAVDLSPAVGQGKTYRVMAVKDAFGTPVAQGTYNGGTVN
                     IPTPSEFGVFLVLSD"
     gene            1965..2840
                     /locus_tag="PK710_19710"
     CDS             1965..2840
                     /locus_tag="PK710_19710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12012.1"
                     /translation="MSKSAKPNAVPKLGTPAPDEFKKLIRTTALRVGVPILVVWIVAL
                     LIGHPYVLIGAGVLTLAALGIVAWAYNYLRKGRAVANILHSADMTSASGRKEAMDKLA
                     DKADKGDTAALFAKAQLMMHEDPRKALEVLEQINLDKVMAPIADEARGQRAMIHLLLA
                     EVDRARTLVDGIDLSRHDQLKSRAALAAVVAEAWARSGQGKRANETLDLFDPNNSELT
                     EVKPQLFRSRAFAAASQSDLKRMRHALRQLKAINPQLLGGFVQKKIHPLLEREARQML
                     AQSGAIPKKMVRRRP"
     gene            2837..3328
                     /locus_tag="PK710_19715"
     CDS             2837..3328
                     /locus_tag="PK710_19715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12013.1"
                     /translation="MNSPKSSVLLAQLEALYQQVDAALQGWTCQASTLCCRFGITGRE
                     PYVTSIELALIERQLASRGGGLPTRRRALPLARDTVEQFGQRWRKEERTCPLLDSSGR
                     CFVYACRPFGCRTFFCDNSLKSKHISRERIRQWVAEIERLAHHYCPDGDKGRPLTRAL
                     RVG"
     gene            complement(3359..>4850)
                     /locus_tag="PK710_19720"
     CDS             complement(3359..>4850)
                     /locus_tag="PK710_19720"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOT12014.1"
                     /translation="GSIDGLQAKHRRAQLARESQFYGAMDGAMKFVKGDVIASLVITL
                     INILGGLTVGVLQRSMDATTALKRYGLLTIGDGLVTQIPALVLATAAGILVTRVASEE
                     ADASLGEELAEQLFGTPKAMRVAGIFVLLLALVPGLPWIPFLLIGAVLLLVSRTRATQ
                     LRKKNEQHELRMAQPRTTARGGKGPTFIPMVAPWSVHLGEGLAGLMEDERKGDEVLLA
                     GLRTRGTTIRERLFAELGVPVPPAVMQVSQQLPAQRAVLSIFEIPYCAFDLSEPQDPA
                     SKLDTLETMLVESLRRRASDFLGIAETQMLLDQLEQFAPPLSRQVVPKPISLTILADV
                     LRRLVEEAISVRDLRSILEALALAPATDKDPLSLAEFVRGHLRRALTFQLTAGVTSLP
                     AYLLDPEVEETIRNAVQRTPSGNFLALSPNVARQIVSALLQMINDSPPPEGRPVVLLT
                     QPDVRRFVRKLVETSIPQMHVVSYAELMPEIALQPLGRTTTSAAPA"
BASE COUNT         1052 a         1307 c         1443 g         1048 t
ORIGIN      
        1 gggtgactgg tatcaagcgc ctccaactcc aagccgggag ttttacgtgt cgccaaccgg
       61 ctcaggcgac gggtcgagcg tttcgtctcc tatgagcttc cacgcagcca tacaacaagc
      121 caaggagggc gattggtact ggatgttgga aggcacgtac aaggattttt accgtattat
      181 cgaggacggc aaagcctcca agccgattgt ttttcgcaac tacgccaatg ggcgagcgcg
      241 catcgatggc aatatccaag tagaaggaga atacaattgg ttatggggtt tggaggtcga
      301 taacctctat ggaatcacca acgatgtcgg tgatggtgaa aacatcggga tgtttagaag
      361 ggggattcgt atcatcaact gcatcgtgca cgacgggtcg agtacttttg gttcgtggaa
      421 ctttcccgag caggtcattt acggcaacat catttatcgg ggataccatt ccatctatac
      481 gcaaaacacg tatagcaacg gtgtgaagta cttcgtccac aacataagca tggatgcctt
      541 tacggattcc ggcaatggtc cttacgaatt tcatgcttac ggggaaggag gcgatgtgag
      601 cggtctgtat ctcgagggga acgtatttgc gaatacggac cttgagggaa agagtctcat
      661 tggagggaaa aacgatactc ccaacgacca catgactctt ataggaaact acttttatgg
      721 ctcgcacgct cagcttggct atgcgcggcc cgtccaagcg gttttcaaag ataacaagtt
      781 gtataaagca tcgctgcgct ggagatactt ttggggcgat ggcaatgagc gatttcccaa
      841 tccgcctccc gcggtcgtga cgggaaatac tttctatcag ccggatggtg gggaccccca
      901 tgtccagttc cagacttcga cctacaaggc ctgcagcgat ccaaagggat actgccgtga
      961 ggacggtacg tcgaagatcc gtcccgacga cgtttgggat cagaatacgt atttgccgaa
     1021 tttccgagcg gattttcatg ccaacaacca gaacatcgag ggcatgactt cgttgagcca
     1081 atggcaaaat gccacacaat ccgcgggcaa gatgtttgac tcgaattcga cggtaaccgc
     1141 catgccgacc cagccgatgg ttgcggttct tcccaatgcc tatgagccgg ggcgtgcgca
     1201 cgtggtgatt ttcaactttg ccaaggcgag caccacggcg gtcgatcttt ctcctgccgt
     1261 gggtcaagga aagacctacc gtgtgatggc cgtgaaagac gcgtttggaa ccccagttgc
     1321 tcagggaacc tacaacggtg gaacggtgaa tatcccgact ccgtcggagt ttggggtgtt
     1381 tttggtgctt tccgactgag aaggtgttgc cagcttgacc gagcccttcc taattccctg
     1441 atggacgtgg tcgttgaacc caggttgacc agccctaatt aaaatatcaa taaatacggt
     1501 tagttggccg cactgtggtg atatttgcgg gctaccacgc cttctgccga agctcggcga
     1561 ggtattcctc ccgcgcaagg tgtgtccttg tcagcccagc caggatgcgc tgctgttcag
     1621 ttgggagggt actccgcagc cgcatggcac cctccgcttg tcgttcgacc aagggcaaga
     1681 gcagccgcga accccgaaag aaggctgata ggtcgaagtc caaaggtcat acgctggcga
     1741 gcgtcgtgct accctacttt tccgacgacg gtacacgcgc tggcgcgcct gcggcgtggt
     1801 gacttttcca gttttccacc gggtctgccc cgaacctttg caccagattt tgtgaatgac
     1861 tgacgcggtt gggtcttccg gtcttgccag caagggcgcg acgaagctag ataggggcct
     1921 ttcgttacct ttatgcccga acgacgggtc gagcaacggc agccgtgtcc aagtcagcca
     1981 aacccaatgc cgtgccgaaa ttaggcacac ccgctcccga tgagttcaaa aagctcattc
     2041 gtaccacggc cctgcgtgtg ggcgtcccca tcctcgtggt gtggatcgtc gcccttttga
     2101 tcgggcatcc ttatgtactc attggtgcgg gggtattgac gctggctgct cttgggatcg
     2161 tggcttgggc gtacaactat cttcgcaagg gcagggcggt tgcgaatatt ttgcattccg
     2221 cggatatgac cagtgcctcg ggtcgcaagg aggcgatgga taagctggcg gacaaggcgg
     2281 acaagggcga tacggcagcc ctttttgcca aagcccagct catgatgcat gaggatccga
     2341 gaaaagcgct ggaggtgctc gaacagatca acctggacaa ggtgatggcg ccgattgccg
     2401 atgaggctcg gggacaacga gcgatgattc accttttgct cgcggaagtg gatcgcgcgc
     2461 gcacgcttgt cgatgggatc gatttgagtc ggcatgatca actcaagtcg cgtgcggccc
     2521 ttgccgcggt cgtggcggaa gcctgggccc ggtccgggca aggaaaacga gccaatgaaa
     2581 ccctcgatct tttcgatccc aacaacagtg aactgaccga ggtcaaaccc cagctttttc
     2641 gctcccgggc gtttgccgcc gcttcgcaaa gcgatctgaa acgcatgcgt cacgccctgc
     2701 gtcagctcaa agccatcaat ccgcaactgc tgggcggctt cgtgcagaaa aaaatccatc
     2761 ctttgttgga gcgggaagca cgtcaaatgc tcgcgcaatc cggagccatt cccaagaaaa
     2821 tggttcgccg tcgcccgtga attcccccaa atcctcggta cttctggctc agctcgaagc
     2881 cttgtaccag caagtggatg ccgcgttgca aggatggacc tgtcaggcct ccacgttatg
     2941 ttgtcgcttt ggaatcacgg gccgggaacc ctatgtcacc agcatcgaat tggcgctcat
     3001 cgagcgacag ctcgcttcac ggggcggcgg tttgcccacg cggcgtcgag ccctgcctct
     3061 cgcgcgcgac acggtggagc aattcggaca acgatggcgg aaggaagagc gcacctgtcc
     3121 gcttctggat tcatccggac gatgcttcgt gtatgcttgt cgacctttcg gatgcagaac
     3181 cttcttctgt gataactctt tgaaatcaaa gcatatttca agagaacgga tccggcaatg
     3241 ggtggccgag atcgaaaggc ttgcccatca ttattgtccc gatggagaca agggtagacc
     3301 gcttacacga gcccttcgcg ttggatagat gaccaggcca ctccgtggcc gatgagcatc
     3361 aagccggtgc agcgctggtc gtcgttcgcc ccaacggttg aagggcaatt tccggcatca
     3421 attcggcata agagacgaca tgcatttgcg gaatactcgt ttccacgagt tttcgaacga
     3481 agcgacgcac gtctggttgg gtcaacagca cgacggggcg tccttccggc gggggagaat
     3541 cgttgatcat ctggagcagt gcggagacga tctgccgggc gacgttgggg gatagggcaa
     3601 gaaaattgcc ggaaggagtg cgttggaccg cgttgcgaat cgtctcttcc acctctgggt
     3661 ccagcagata agcgggcagg gatgtgacgc cggcggtgag ttggaacgtc agcgctcgtc
     3721 gcagatgacc gcggacgaat tcggccagcg acagcgggtc tttatccgtg gcaggggcca
     3781 gagcgagagc ttccaggatg cttcgcaagt cgcgcacgct gattgcttct tccacgaggc
     3841 gtcgcaacac gtcggcgaga atggtaaggg aaatgggttt gggaacgacc tggcgcgaca
     3901 acgggggcgc gaattgctcg agttggtcga gcagcatttg ggtttcggcg atgccaagga
     3961 agtccgacgc gcgtcggcgc agggactcga cgagcatggt ttcgagcgtg tcgagcttcg
     4021 aagcggggtc ttggggctcc gaaaggtcga aggcgcagta cgggatttcg aagatggata
     4081 aaacggctcg ttgggcggga agctgctggg atacttgcat gacggctggt ggaacgggga
     4141 cgccaagttc tgcaaacagc cgttctcgaa tggtggtgcc gcgtgttcga aggcccgcga
     4201 gtagtacttc atcgcccttg cgctcatctt ccatcaaccc ggcgagtccc tcaccgagat
     4261 gtacggacca gggagccacc ataggaatga acgtaggtcc tttgccaccc cgggcagtgg
     4321 ttcgcggctg agccattcgc agttcgtgtt gttcgttttt ttttcgaagt tgcgtggcgc
     4381 gtgtgcggga cacgagcaac aacaccgcgc cgatgagcag aaatggtatc cacggaaggc
     4441 ccggaacgag agccagaagc agcacgaata tgcctgctac gcgcatggct ttgggggttc
     4501 cgaaaagctg ttccgccagt tcctctccca acgaggcatc cgcctcttca ctcgccacgc
     4561 gggttaccaa aatccccgcg gcggtggcca acacgagcgc tgggatctgc gtcaccagcc
     4621 catcgccaat cgtcaacaat ccatagcgtt ttagcgctgt cgtcgcatcc atgctccgtt
     4681 gcaacacccc gacggtaagg ccgcccaaaa tattgatgag cgtgatcacg agggaggcaa
     4741 tcacatctcc tttcacgaat ttcatcgccc catccatggc gccgtaaaac tgcgattctc
     4801 gtgcgagttg tgcgcgacgg tgcttggctt gaagtccatc gatgctgccg
//
LOCUS       DAOSOR010000465         4846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_17577_length_4846_cov_3.639115, whole genome shotgun sequence.
ACCESSION   DAOSOR010000465 DAOSOR010000000
VERSION     DAOSOR010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17577_length_4846_cov_3.639115"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            554..949
                     /locus_tag="PK710_19725"
     CDS             554..949
                     /locus_tag="PK710_19725"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOT12015.1"
                     /translation="MHAEINSLPAPRPLPSRQLPGGKKHDDSPPIVVQLREFSEANLY
                     AGLNADEVLGVFVPTYRPWAVGTLVPVALDIPGRDDPLVVHSVVRWTRQGNSDTYPGV
                     GLEFLHLDAKAKKRLSRFILTRTPMLFEI"
     gene            978..1532
                     /locus_tag="PK710_19730"
     CDS             978..1532
                     /locus_tag="PK710_19730"
                     /inference="COORDINATES: protein motif:HMM:NF022591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HOT12016.1"
                     /translation="MRTRFFPLLLMVAVAVSCHEKEDASSASAISSHTPEAPNPSLSV
                     PPVIPSTSNSAAPVAEQLNTVALELQELTFTSDVNNKEPVDVLLTAETGKRVWVHLRL
                     RNRGENPRKIIVQFTVNGEKRTKVELQVDKSWSYRTWAYNTLRAGDKTGKVQVTVTDD
                     EGVNLADTSLPIANKAQTKGYGKK"
     gene            complement(1717..3285)
                     /locus_tag="PK710_19735"
     CDS             complement(1717..3285)
                     /locus_tag="PK710_19735"
                     /inference="COORDINATES: protein motif:HMM:NF018530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HOT12017.1"
                     /translation="MSASSELLATMAVIAAGAMLGRLQIHGIRLDVAAMLLLGVGVAH
                     FGVTLSPALGLFGLLLFLYTVGVRSGPALRNMDRRDFQLAAVGFGIILVAMGGVVSLG
                     RLLGVPLLVSLGSYAGFFGSGAALALLEGAAGGGGASAAFAVSAPIGAVFIMVLVQIW
                     LALSQKRITGELDEWNRTMAAEQEHSESATLLVEQDEVLGQSLRDLRLGCQVLWVTRD
                     KTSIAASADTVLMKNDVIHVSGSREGVENTIKRVGRGIPPLPASSAQIVVRKFFVSNP
                     RAIEKRLEDLLLRERYGATVTRIRRAGINLRAHHGFRFRWGDRIQVSVPADRIDDLRQ
                     LFGDDAHGLETFAFPRAALVIFVGGLLGALPIQLGAAGSLRLGPALGVLVVSLITSAL
                     HRTGPMVWSQSGPTTRLMSQIGLPLFLAQIGNESYTGLVQSWDEYGSILFVLAVAPIL
                     FVALLVVLAGRLFRYGPLTVLSLLPTVAMNTPALINLQDKFREKIPSHVYATVYPVVA
                     LGLLLVMFGLSFWV"
     gene            complement(3357..>4846)
                     /locus_tag="PK710_19740"
     CDS             complement(3357..>4846)
                     /locus_tag="PK710_19740"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT12018.1"
                     /translation="SDEATRCVIEINTQNSVEILARSREAQGLWAAKSAVDRAHRLRS
                     LSTIILERAEQFAQAIHDDTGMLITDALALEILPAARLIDQWSLQAESLLESSPLEFN
                     PLVLPRKSARIERAPRGVVAVLTSSLSPLLSPIRHGLPALLSGNALWLKPDERTPRTT
                     NLIASSLSGLLPADVVFVVPGGAEVGEFIIKSGVAAVFFSGGLDAAKRVAVCCAHDFI
                     PCFVEVLRKTGAVVLDDASIERTARGLVWAAFAKGGQGVHSLGHVFVHQAIADSLAER
                     IATLTKELRAGIDHAPPPLQQRSQALLTQLSTDNEGITVLAGGKSDPEQNVFPPTVVR
                     VALEQVSWLPSFEKAPVLSLVVVKDEKQALELAKNSALGPTISVWTRHAERGQEIAQG
                     LRENVWTVNHHGVPSPLVGMPWSTTERTLDLIVSGSSDFDKHSCSKLVLVDRCTRSRE
                     LGWYPYDDTYQRMLVETARLQSGGWLGRLMALLRLLVLYPKRWLS"
BASE COUNT         1192 a         1482 c         1264 g          908 t
ORIGIN      
        1 acttctcggt aaccatcgac gtctccttca ggtctatcct caagcgttga ggcgcgagag
       61 gaaagcactg ctcatgccaa gggtggacga acccaatcga ggcgaaatga ccaggcatct
      121 gcgacatgag tgactcgact tggcatcgat agaccatcgt cctgggtcga gcttgtcgca
      181 gtcgttgccc aatcgacccc tttgatacaa gcaattcacg aaaaatgctc cgcttcaggg
      241 cgcccggacc cccttcatcc cgactgcagt gcccatttcc gcccgcaagg tcagcacgta
      301 ggatatttat gccttctcga cctcccctac cgtccagaaa acaaagaaaa tcacgaaaaa
      361 tataaaggtt ggactaccga accgtgggat gagttcaagc tccaccggcc caacccccgg
      421 tgcctcgctg cctgcgatgc aatatgacgc cgggctgcgc ctcctgactc cagtctgtgc
      481 gtcgagggaa gctttcctcc cgtatcaacc aaaaatttgg cccttcctgg cccatgccta
      541 ccatcgcccc atcatgcatg ccgaaatcaa ctctttgccc gctccgcgcc cccttccttc
      601 ccgccagctc ccaggcggaa aaaaacatga tgattcccct cccatcgtcg tgcaactccg
      661 cgaattttca gaggcaaacc tatacgcggg cctgaacgca gacgaagtgc tgggtgtgtt
      721 tgtccccacc tatcgcccat gggccgtcgg aacactcgtg cccgtagcgc tggatattcc
      781 cggacgcgac gacccgctgg tcgtccactc ggtggttcga tggacgcgac aggggaattc
      841 cgacacttat cccggcgttg gactcgagtt tttgcacctc gatgccaaag caaaaaaacg
      901 gttgtcacga ttcattctca ctcgaacacc catgctattc gaaatatgag cgtttgttcc
      961 tcacccaaag gattcgaatg agaacccgtt ttttcccctt gctcctgatg gttgccgtag
     1021 cggttagttg ccacgaaaaa gaggatgcct cctccgcttc cgccatctct tcccacaccc
     1081 ccgaggcgcc aaacccctca ttatccgtac ctcccgtcat cccctcgact tccaactccg
     1141 ccgcccccgt tgcggaacag ctcaataccg tcgcgctcga attgcaagaa ctgactttca
     1201 cttccgatgt gaacaataag gaacccgtcg acgtgctcct caccgccgaa accggaaaac
     1261 gcgtctgggt tcatctccgc ttgcgaaatc gtggagaaaa ccctcgaaaa ataatcgtcc
     1321 aattcaccgt caacggagag aaaaggacga aagtcgagct gcaagtcgat aaatcatggt
     1381 cctatcgaac ctgggcctac aatactcttc gggctggaga taaaacaggg aaagtgcagg
     1441 ttacggtgac cgacgacgaa ggtgtcaacc ttgccgatac gagcctgccc atcgccaata
     1501 aggcgcaaac caagggatat ggcaaaaagt agtctcgctt cgccaatatg cctatccgaa
     1561 tcccgatatt tcctcgataa gaataccgcg taacatcatt tcttcccggg actcattctt
     1621 cggaaccgat aaaaaatgtg taataacaaa tagttgtacg gtttctgcca ggatggaaac
     1681 cttctgggca acgaagcggc cttcctatcc cctccctcat acccaaaacg atagaccgaa
     1741 catcacgagc aacagcccca aggccacgac aggatacacc gttgcgtaga catgacttgg
     1801 gattttttcc cgaaacttat cctgcaggtt gatgagagcc ggcgtgttca tggcaaccgt
     1861 cggcaaaagc gataacaccg tcaacggccc ataacggaaa agccgtcctg cgagcacgac
     1921 caacagggct acgaaaagaa ttggagcgac cgcaaggacg aaaaggatag agccgtattc
     1981 atcccaggac tggacgagac cggtatagga ttcattgccg atttgcgcga gaaatagagg
     2041 taatcctatt tgtgacatca gacgcgtggt ggggccggat tggctccaga ccatgggacc
     2101 agttcggtgc aaggcagagg tgatcaacga aacgacaagc accccaaggg cgggtcccaa
     2161 acgcagggaa cccgccgcgc caagctggat tggcagcgcg ccaagcaaac cgcccacgaa
     2221 aatcacgagc gccgcccggg gaaacgcaaa tgtctccaaa ccatgcgcat catcgccaaa
     2281 caactgacga agatcgtcga tcctatcggc cggaacactc acctggatac ggtcccccca
     2341 gcgaaaacga aaaccatgat gcgcacgaag attgatcccc gcacgacgaa tcctcgtcac
     2401 cgtcgccccg taacgctcac gtaacaatag gtcttccaaa cgtttttcga tggctcgcgg
     2461 attggagacg aaaaacttgc gcacgacgat ttgcgcgctg cttgcgggca ggggagggat
     2521 accacgcccg acgcgcttaa tcgtgttttc gacgccttcc cgtgagccac tcacgtggat
     2581 cacgtcgttt ttcatcaata ccgtatcggc ggaagcggca atgcttgttt tgtcgcgcgt
     2641 gacccacaga acctgacagc caagacgaag atcgcgcagg gactggccga ggacctcatc
     2701 ttgctcgacc agcagggtag cggattcgga atgctcctgt tcggcagcca tcgtacgatt
     2761 ccattcgtcg agttccccgg tgattcgttt ctgggagagg gcaagccaga tttgaacgag
     2821 caccatgatg aaaacggcac ctatcggagc agaaaccgcg aacgccgccg acgcaccgcc
     2881 tccccccgcg gctccttcga gcaacgccaa ggcggccccg gaaccaaaaa atccagcgta
     2941 agatccgaga gatacgagca aggggacccc cagcaaacga cccaacgaca ccacccctcc
     3001 catcgccacc aggatgatcc caaaccccac ggccgcaagc tgaaagtcac gtcgatccat
     3061 gttgcgaagc gccggacctg agcgaacgcc gacggtgtat aaaaacagaa gcaaaccaaa
     3121 taagccaagg gcgggagaaa gcgtgactcc aaaatgcgct acgccaaccc caagcaaaag
     3181 catggccgcc acatcgaggc gaatgccatg gatctgcaac cgacccagca tcgcccccgc
     3241 ggcgatgacg gccatggtcg caagcagttc agaagacgcg gacatgccgc gccacatagg
     3301 acaagcttgg cccgttgtcg acggcggtct gcagctttct cgaatgtatc aagtgctcat
     3361 gagagccaac gcttcggata caacacaagc aaacgcaaca aggccatcag cctgcccaac
     3421 catccgccgc tctgaagccg ggctgtttcc accaacatcc gctgatacgt atcatcgtac
     3481 ggataccaac caagctctcg cgaacgcgtg catcggtcca ccagcacgag tttcgaacac
     3541 gaatgcttgt cgaaatcaga cgacccgctc acgatcaaat cgagggtccg ttcggtcgtg
     3601 gaccaaggca tgcccacgag gggcgacggc acgccgtggt gattcacggt ccacacgttt
     3661 tcacgaagcc cttgggcaat ttcttgtccc cgttcggcat gacgcgtcca cacactgatg
     3721 gtcggcccca gagccgagtt tttcgccagt tccagcgctt gcttttcatc cttgaccacc
     3781 acgagggaca atacgggagc cttttcgaac gaaggcagcc atgacacctg ttcgagcgca
     3841 acgcgaacta cggttggcgg aaagacattc tgctccggat cggatttgcc tccagccaac
     3901 accgtgatgc cttcgttgtc ggtggaaagc tgcgtgagaa gcgcctgcga tcgttgctga
     3961 aggggagggg gggcatggtc gattccagct cgaagctcct tcgtaagcgt cgcgatccgc
     4021 tccgccaacg aatcggcgat agcttggtga acgaagacgt ggccaagcga atgaacgcct
     4081 tgtccccctt tggcaaacgc cgcccacacc agcccccgcg ccgtgcgttc gatcgaagcg
     4141 tcgtccaaaa cgaccgcccc ggtttttcgc aacacctcga cgaaacacgg gatgaagtcg
     4201 tgggcacagc aaaccgcaac gcgcttcgcc gcgtcgagcc ctccggaaaa aaataccgcc
     4261 gcgactccag atttaattat aaattcgcct acttccgcgc cgccggggac gacgaaaacc
     4321 acatccgcgg gcagcaatcc cgacaacgat gatgcgatca gattggtggt tcggggcgtt
     4381 cgttcatctg gtttgagcca tagggcgttg ccggagagca gcgcgggcag gccatggcga
     4441 atgggcgaaa gcagaggcga tagggatgag gtcaacacgg ccacgacacc ccggggcgcg
     4501 cgttcaattc ttgctgattt tcgaggcaat acgagcggat tgaattcgag aggggatgat
     4561 tcgagcaagg attcggcttg caacgaccat tgatcgatga gccgggcagc gggaaggatt
     4621 tccaacgcaa gagcgtcggt gataagcatc ccggtatcgt cgtggatggc ttgcgcgaat
     4681 tgttccgcgc gttccaaaat gatggtgctg agcgaacgca atcgatgagc acggtccacg
     4741 gcggatttgg ctgcccacag cccttgggct tcgcgggatc gggccaagat ctcaacgcta
     4801 ttttgcgtgt tgatttcgat gacgcaccga gtggcctcgt cagagg
//
LOCUS       DAOSOR010000466         4834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_17629_length_4834_cov_3.764804, whole genome shotgun sequence.
ACCESSION   DAOSOR010000466 DAOSOR010000000
VERSION     DAOSOR010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17629_length_4834_cov_3.764804"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PK710_19745"
     CDS             complement(<1..183)
                     /locus_tag="PK710_19745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HOT12019.1"
                     /translation="MHEDIFGLMGTIVNNQYRVDSVVGEGGFGVVYRGWHLSFECPIA
                     IKCLKIPPHFTTEAKGQ"
     gene            1076..2884
                     /locus_tag="PK710_19750"
     CDS             1076..2884
                     /locus_tag="PK710_19750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12020.1"
                     /translation="MAMPAFADPPPGQSAWLNGMHDIESLGYMQNATSGCDKGWITDL
                     QYIGAGGQPSTNCHDAAVAAGISIIQRLDVDGSHSFPLDPAQAPGYASAFASFASQCP
                     NTHVWIVGNEPNFTTNNADPESYASPYAEAYAKVHAALHAISGHHNDLVLVAPASPYS
                     PFCICSMHRIIQQIVARGVQPDGFAIHAYTQAQHSGDYGSLASLVTSEDMSASNDGCG
                     YPFHWQFRIYRDWIAAIEKEGHGGKPVFITETGNACAPQQGNSCYPNQDVGYFQAMFQ
                     EINAWNQDPSHTTKIRAITPYRWTLNDDGTGRDFAIGNRPALLDDLTHAFSKKYAWTT
                     PASCESQPNTCTDDASCSGSTICDLTKGNCASISSCGNGGVCPTGEICREATFDCAPS
                     TRGAANITFEPSNPAPGSEVTIDVSGTAGYTNVGLSFEAVPGATVPTELVAIEGTGPY
                     HWKYKATAGATGTYRATFVADPGASTVYAIGYVNVGDVFPDTGGSGGAGGTGGAAGTG
                     GMGTGGSGGISESGGASGSGGGTAGSSGGAAGSSHQTYTNDTNDEGCGCTMPGVGASQ
                     PGYPAILALIALHWAQRRRRSTESDRSPHYFLKNKG"
     gene            3318..4046
                     /locus_tag="PK710_19755"
     CDS             3318..4046
                     /locus_tag="PK710_19755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12021.1"
                     /translation="MRRRCQTRAGRHNPGRWLPLAILTIATGACGPSHNVIAGGNTVP
                     STNETGTSPAARDVGANGSASAPPAPSAAPTPVPPPPPESLAERYATYCPEVVELAAK
                     GDPEPTTSVSRENVSATIKTVKNGLAASVTMPRAFLPPIEREVLWVAQGGDPYECCAK
                     SSPSGLDMMCSLSSVDYIEGRATIRREGNTLIVRWCMADLDTRTVLDRGEIRTQLSDG
                     TALRFVAPETACKGFPANLGGGSK"
     gene            4064..4222
                     /locus_tag="PK710_19760"
     CDS             4064..4222
                     /locus_tag="PK710_19760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12022.1"
                     /translation="MIRSWALSFLVPRFAARQRLLPHDRQRRWAGEQGLSHLLMNGYH
                     VASLSEDP"
     gene            complement(4284..>4834)
                     /locus_tag="PK710_19765"
     CDS             complement(4284..>4834)
                     /locus_tag="PK710_19765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12023.1"
                     /translation="PGAYEEGGAVAGGLGNTVRVDPDTQSFVLDDWIVMDPRTDATRL
                     RADFHLGDPRDWEVGSHPIERLYQDRRRPETFWEVRLYFDDTACKSCRSCEAFVNGVA
                     FEVLEAGGSLTPYPEVVSEDFRRRYRVRLDVHDLVGHRYEEGGPVECASRASLVFDLT
                     FERTSSDFLIEPDRFCNQYSTL"
BASE COUNT         1049 a         1489 c         1366 g          930 t
ORIGIN      
        1 ctgccccttc gcttccgtgg tgaaatgcgg tggtatcttc aagcacttga tcgcgatagg
       61 gcactcgaaa ctcaggtgcc agccgcgata cactaccccg aatcctcctt caccaacaac
      121 gctatccacc cggtattggt tgttgacgat cgttcccatg agtccgaaaa tgtcctcgtg
      181 catcgtgagc gtacgatacg ccaagaccaa gccctgggtc tacgattttc catggctcct
      241 ttgaaatggc tgttcggcga tgacgccgat ggcacgaagt ctcccgccgc agcccttccc
      301 cacggccagg ccagcatcag gggaacaaag gcgccatggc cgaatccacg cctcgcatcc
      361 agcgccgtcg caaatccaaa tccttttaaa ttcaaatact tgacatgcca gcgcgggcgt
      421 tcctggcgcc tcccagacaa gctcctacga ctccgaacga aaggaacgga aagcgagcga
      481 tttgaggagg acatggggta gccaagccag aatatgcttg tttctaacga gctgggcggc
      541 ataggtccag agccccgggt gtcgttggga ccatcgtgtc tgccaacgcc tgtcgatggg
      601 ccacggcgcg ggcactttgg caaagcccat ggaatcactg cgattatctg tcacatcctc
      661 tgaaaacctg gtcattcgca gcggatgcac acggccattg acgtccgtca tgcggatcac
      721 gacaaaacgt ccctccagag tacgatacaa cgacgcggca ttcaaccgta gaggcgttca
      781 tgcgtcaagg gatagaccag gaaagccgga aaccgaggtt gttatcgcgg taaccgggac
      841 cgctgtcgta ccgaatcgca gagcggcaga gcgccgcgta gccgaaccaa gaaacaccac
      901 gccccacccg atccatgccc gtctgtgctc ccaaagtttg aaaaatcagg ttgtcgaagc
      961 gatggtattt cgatatccta catacatggc tatctctcat tccattcctt cggtttggaa
     1021 ggcgctgcgc gtgattcgcc cacaaatagt agcggtctcg gcaggggtcc tgacaatggc
     1081 tatgcccgct ttcgccgatc caccaccggg gcaaagtgca tggctcaacg gaatgcacga
     1141 tatcgagtcc ctgggctaca tgcagaatgc cacgtcgggt tgtgataaag gatggatcac
     1201 tgaccttcaa tatatcggcg ctggaggtca accttcgacc aactgccacg acgccgcggt
     1261 tgccgcggga atttccatca ttcagcgcct cgatgtcgat ggcagccact cttttcctct
     1321 cgatccggca caggctcccg gctacgcaag cgctttcgct tcctttgcct ctcaatgccc
     1381 caacacccat gtctggatcg tgggcaatga gcccaatttc accaccaaca acgccgatcc
     1441 ggagagttac gcctcacctt acgccgaggc ctacgccaaa gtgcatgctg cgcttcatgc
     1501 catttcgggt catcacaacg acctcgttct cgtcgccccg gcgagccctt attctccgtt
     1561 ttgcatctgc tcaatgcacc ggatcatcca gcagatcgtg gcgcgcggcg tccagcccga
     1621 tgggttcgcc attcacgctt atacacaagc ccaacattcg ggtgactatg gctccctcgc
     1681 ctctttggtc acgagtgaag atatgagcgc ttccaacgac ggttgcggtt atccattcca
     1741 ctggcagttc cgcatttacc gcgactggat cgcggcgatc gagaaggaag gccacggcgg
     1801 aaaacccgtc tttatcacgg aaaccggcaa tgcctgcgcc ccccaacaag gcaattcctg
     1861 ctatccaaac caagatgtag gctatttcca ggccatgttc caggaaatca acgcctggaa
     1921 ccaggatcct tcgcatacga ccaaaatccg cgccattacg ccttatcggt ggaccctcaa
     1981 tgacgatggc actggccggg acttcgcgat tggcaaccgt cccgcccttc ttgacgacct
     2041 gactcatgct ttctcgaaaa aatacgcctg gacgacaccc gcgagttgtg aatcgcagcc
     2101 caacacgtgt acggacgacg catcctgcag tggatcgacc atttgcgacc tgaccaaggg
     2161 aaattgcgct tcgataagtt cgtgtggcaa cggtggcgtg tgtccaacag gcgaaatctg
     2221 ccgcgaagcg accttcgatt gtgctccctc gacccgaggc gcggcaaata tcacattcga
     2281 accgtccaat ccagctcctg gaagcgaagt gacgatcgac gtatctggca cggccggata
     2341 cacgaacgtc ggtctttcgt tcgaggccgt gcctggagcc accgtgccga cggagcttgt
     2401 ggctatcgaa ggaacgggcc cttatcattg gaaatacaag gctaccgcgg gagccacggg
     2461 cacgtatcgg gccaccttcg tcgctgaccc tggcgcaagc accgtctatg cgattggcta
     2521 tgtgaacgtg ggagatgtct ttccggatac cggaggctcg ggaggggctg gcggcacggg
     2581 aggcgcggcc ggtacaggcg gtatgggaac cggaggcagt ggaggcatca gcgaatccgg
     2641 cggtgccagt ggctcgggcg gtggcacggc agggtcgagc ggtggtgcgg cgggttcttc
     2701 tcaccaaacc tacacgaacg acaccaatga cgagggttgc ggctgcacaa tgcccggagt
     2761 cggcgcatcc caacctggtt atccggcgat actcgcgctc attgcactgc actgggcgca
     2821 gcggcgccgc aggtccacgg aatctgatag atcccctcat tatttcctga aaaacaaggg
     2881 ataggggaga caatccccta aaccttgaca ttgagccggt ccttgccatg aacttgaacg
     2941 tgaacttgga ccccaaaacc ttgcgttgga catcgtcatg aacctgagcg attctggctg
     3001 agtggtgatg ggtacgactt tccggcagtc gataatcagg gaaatagtcc gggaaaaatg
     3061 aggggacggc tcaaacctcg cacaggatga atacttcgcc gagcttctgg agaaggcata
     3121 ggaacccacg agcatcaccc tacagtgctc aagcaattgt atttattgat gtttttacac
     3181 cgcccccctt cggaaacggg gtttgcggag atacccgatg tgatggctgg cttttccaag
     3241 acggcatcgc aggcagacgg tctgtggatg aaaggaaagt ggttgatgag ccacttccgc
     3301 cggagtatct tttgaagatg aggcggcgtt gtcaaacgag agctggcagg cataatcccg
     3361 gtcggtggct accgctggct atccttacca tcgccactgg cgcgtgtggt ccgtcgcaca
     3421 acgtcatcgc cggggggaat accgtaccgt ccaccaacga gacgggaacc tcaccggccg
     3481 cgcgtgacgt gggtgcgaac gggagtgcat cggcccctcc cgcgccctcg gcggctccaa
     3541 ccccggtgcc tccacctccc cccgaatctc tcgccgaacg ctatgcaacg tactgcccgg
     3601 aggtcgtcga actcgcggcc aaaggcgatc cggagccgac gacgagcgtg tcgcgagaaa
     3661 acgtcagcgc cacgatcaag accgtgaaaa acggcctcgc cgcgtcggtc accatgccgc
     3721 gcgcattctt accgccgatc gagcgcgagg tcctctgggt cgcgcaaggc ggcgacccct
     3781 acgagtgctg cgccaagtcc agcccgtcag gcctcgacat gatgtgctcg ctcagcagcg
     3841 tcgactacat cgaagggcgc gcgacgatcc gccgggaggg caatacgctc atcgtccgct
     3901 ggtgcatggc cgacctcgac acgaggacag tgctcgaccg aggtgagatc cgaacgcagc
     3961 tcagcgacgg caccgccctt cgcttcgtgg cgccggagac cgcatgcaag ggttttccag
     4021 cgaatttggg tgggggctcc aaatagaagc aaaagagcca gtgatgattc gctcatgggc
     4081 tctttccttc ctcgttcctc gtttcgccgc tcgccaacgc ttgctccccc acgaccggca
     4141 gcggcgatgg gcaggcgagc aggggctatc ccaccttctg atgaacggat accacgtcgc
     4201 atcgttgtcc gaagaccctt gaacatataa cgttgtggcg cggttcgatg cgtggcgcaa
     4261 actcgccgac caaggagaat caatcaaagt gtcgaatatt ggttacaaaa tcgatccggc
     4321 tcgatgagga agtccgaaga cgttctttcg aaggtcaagt cgaacacgag gctggcccgc
     4381 gacgcacact caacggggcc accttcctcg taacggtgtc cgacgagatc atgaacatcc
     4441 agccggacgc ggtatcgtcg gcgaaaatcc tcgctgacga cctcggggta gggcgtgagc
     4501 gagcccccgg cctcgaggac ctcgaacgca accccattga cgaaagcctc gcaggaccgg
     4561 caactcttgc aggccgtgtc gtcgaaatag aggcgaacct cccagaacgt ctccgggcgg
     4621 cggcggtcct ggtacagacg ctctatcggg tgactgccca cctcccaatc cctggggtcc
     4681 cccaggtgga agtcggcacg gagccgggta gcgtcggtcc ttggatccat gacaatccaa
     4741 tcgtccagca cgaaagattg cgtgtccgga tccacccgga cggtgttgcc gagaccacca
     4801 gcaacggcgc cgccttcctc gtaggctccc ggtg
//
LOCUS       DAOSOR010000467         4817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_17702_length_4817_cov_4.595968, whole genome shotgun sequence.
ACCESSION   DAOSOR010000467 DAOSOR010000000
VERSION     DAOSOR010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17702_length_4817_cov_4.595968"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PK710_19770"
     CDS             complement(<1..514)
                     /locus_tag="PK710_19770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12024.1"
                     /translation="MGLRVPDEHATLVDVARPSVTVTILWLTIVAILLGCGVGKERKP
                     PGVPPSPESISREEPGGDASDPHQAALLRLGSEPWGWRNDRQDVFHFPLTDWPHWRRV
                     RFWGLPAFVGFRYGDAHRAIAGLWVRRLRPEDPESLEVCFDRMHAWSQPIASDYQTKL
                     TLGPRSYASWK"
     gene            607..1515
                     /locus_tag="PK710_19775"
     CDS             607..1515
                     /locus_tag="PK710_19775"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOT12025.1"
                     /translation="MSKPLIPAIHLPEIPILPNQHTLDVLGRTMEVGPWTLKPFGVLV
                     AIGVYAGVFIAGKFAKSRGIPQIAMSRFMLWVLVSAFVFGHVLDALFYHPQRVVSDPW
                     MVVRLWDGLSSFGGFVGAAIGVFAFKRHFKVKHVLPFADTMAAAFPVGWTFGRMGCAV
                     VHDHPGMRSDLWFAVAFEKGARFDLGLYEMVFAAGVAIVCLLLARHPRPPGFFLGFTM
                     LVYAPVRFALDFLRAEPGQVAGADPRYVMLTPAQWASILMASAGFTLLWRAADSGEVG
                     NEPFEAAAREADPSLWNQHDDSPEPA"
     gene            1535..2158
                     /locus_tag="PK710_19780"
     CDS             1535..2158
                     /locus_tag="PK710_19780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12026.1"
                     /translation="MSFLPQYQATMAVRTRRSSWVLLCMMGWLGCGGNESPASKPNVT
                     AGPVETMTLSFLNPLTEDLYVPWQDTQPAFELAHGQTELVVHRTCIPFCGNGCVCAPC
                     TKSNRVRKLLPGESLQISWIPIHFVMNSCNDSVSCQCAESWPITAGQYTLSLRGFTDA
                     QGGQPISEHPNILVGASPSSTSQNCQATKVFTLAAKETAVQAQFLCQ"
     gene            2250..3149
                     /gene="prmC"
                     /locus_tag="PK710_19785"
     CDS             2250..3149
                     /gene="prmC"
                     /locus_tag="PK710_19785"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOT12027.1"
                     /translation="MTSQDSPATVSPRTEPWTVRRVLAWVTEDFASRGVETPRLEAEL
                     LLAHVLGANRIQLIVDRDRPLTAHELGAYRALVTRRRNHEPVAYLRKEREFYGHDFEV
                     DARVLIPRPDTETLVEVALKRSQERYLFGRLLDVCTGSGNVALSFAKERPTWQVLATD
                     LSSDAIAVASRNAVRLGVAWNVSFFAGDLFDPVKGQPRFHLITANPPYIPSEQVDQLS
                     PSIRNHEPRLALDGGQDGLDLIRRLIHDAPSWLEQGGELALEMACDQGSRVVEMMTRN
                     GWQDVQIVRDLGQRDRVVRGIRP"
     gene            complement(3561..>4817)
                     /locus_tag="PK710_19790"
     CDS             complement(3561..>4817)
                     /locus_tag="PK710_19790"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOT12028.1"
                     /translation="EGMHVRPNDPSIDPTLARLAAAPIGINTNADFRRFGFGPPEGKV
                     IELLALRPMSVAQVLDLNIMPIRQLKLLLYVLMITRGIVVVPVQTSAPARKPQASEPA
                     VVAPNAPPDSSRTGSEAAQASRQAVPMARVKLKKVAASSTVTPMALSPQDIQQRASQV
                     DAESYFEVLGVDQNASPDVVRSAYFSLAKQWHPDRLPDSYHEVRDDAAKVFARMSDAF
                     QTLSDPDKRAQYVEALEKGGTNKDEQAKVQQVIEATIEYQKAEVYLRKHDLGKALRSA
                     QKAYEGDPEQSHHLALYAWVLSQQPEAQQSKDFETSLRLLDQAIKLDARCEQAFFYRA
                     MLNKQLGKHASALRDFKQVVELNPRNIEASREVRLATMRGGAKALADEKPTSSERTKT
                     KPGASESINWTQDSVSDIFGKLFKKK"
BASE COUNT          961 a         1311 c         1445 g         1100 t
ORIGIN      
        1 atttccatga agcgtaggat ctggggccaa gggtgagttt ggtttgataa tcggaagcga
       61 tgggttggct ccacgcatgc atccgatcga agcatacttc gaggctttcc gggtcttcgg
      121 ggcgcaaacg gcgaacccaa agtccagcga tggctcgatg ggcatcgccg tagcgaaaac
      181 caacgaaagc gggcaatccc caaaaccgaa cccgtcgcca atgaggccaa tcggtcagcg
      241 ggaagtggaa aacatcctga cggtcgttgc gccaacccca aggttcggag ccaaggcgaa
      301 gcaaagccgc ctggtgagga tcgctcgcat cgcctcccgg ttcctcccgg ctgatggatt
      361 caggagatgg gggaactccc ggcggttttc gttctttacc cacaccgcat ccgagcagta
      421 tcgcaacgat cgtcaaccaa aggatggtca cggtgacaga aggtctggcc acatctacca
      481 gcgttgcgtg ttcgtcgggc acgcgcaagc ccatggtgct ttttgggaaa aaagacgatg
      541 ccaagtcgcg cggcaagcat cgagccgtgc tagcactggc gcagcaacgc caacgaaagg
      601 gtttacgtgt cgaagccgtt gattccagcc attcaccttc ctgaaatccc gatcctgccg
      661 aaccagcata cgctcgacgt attgggtcgg acgatggagg tcgggccctg gaccctcaag
      721 ccttttggcg ttttggtggc gatcggagtg tacgctgggg ttttcatcgc tggtaagttc
      781 gcgaaatcac gaggtattcc tcagatcgcg atgtcccgtt tcatgctttg ggttctcgta
      841 tccgctttcg tcttcgggca tgtgctcgac gcgctttttt atcatccaca acgggttgtt
      901 tcggatccat ggatggtcgt gcggctttgg gatggcttat cgagttttgg aggcttcgtg
      961 ggtgctgcca tcggcgtatt tgcgttcaag cggcatttca aagtgaaaca cgtgctgcct
     1021 tttgccgata cgatggccgc ggcgtttccg gtcggttgga cattcggacg aatgggttgc
     1081 gccgtcgttc atgaccaccc gggcatgcgc tcggacctgt ggttcgccgt agctttcgaa
     1141 aagggggccc gtttcgacct gggactgtac gagatggtat tcgcggcagg ggtagccatc
     1201 gtctgcttgc tgctcgcccg tcatccacga ccaccgggtt ttttccttgg attcaccatg
     1261 ctcgtgtacg cccccgtgcg attcgcgctg gactttcttc gcgcggagcc tggacaagtc
     1321 gccggcgccg accctcgcta tgtcatgctg acgcctgcgc agtgggcatc catcctcatg
     1381 gcatccgcgg gattcacctt gttgtggcgt gccgcggatt ctggcgaagt aggcaacgaa
     1441 cccttcgaag ccgccgcgcg ggaggcagac ccatccctgt ggaaccaaca cgacgattcc
     1501 cccgaaccag cttgagctga aaacgcacct ttccatgagc tttctgccac aataccaagc
     1561 caccatggcc gttcgaactc gccgctcgtc ttgggtattg ctgtgcatga tgggatggct
     1621 cggttgcggt ggaaatgaaa gcccggcaag caaacccaat gtcaccgcgg gccccgtcga
     1681 aaccatgacc ctgtcgtttc tcaacccctt gacggaagac ctgtatgttc cgtggcagga
     1741 tacgcagccc gccttcgaac tcgctcatgg acagaccgaa ctcgtggtgc atcgaacctg
     1801 catccccttc tgtggcaatg gctgtgtgtg cgctccatgc acgaaatcca atcgcgttcg
     1861 taagctattg ccgggcgaat cgcttcaaat tagctggatt cccatccatt tcgtgatgaa
     1921 ctcctgcaat gattccgtct cttgccagtg tgcggaaagc tggccaatca ccgcgggcca
     1981 atacaccctt tccttgcgtg gattcacgga cgcccaaggc ggacaaccca tcagcgagca
     2041 ccccaatatc ctggtaggcg catctcccag ctctacgtcc caaaactgcc aagccaccaa
     2101 agtcttcacc ctcgccgcaa aagaaactgc cgtccaagcg cagtttcttt gccagtgaga
     2161 tccgtgccat tgacttcatg ccacgaatcc aacagacggt actccatggt caatgaacct
     2221 cgaatcggat ggagagtcct tcgctcatca tgacttccca ggattcgccc gccactgttt
     2281 cgccgcgtac cgaaccatgg accgttcgac gggtactggc gtgggtcacg gaggattttg
     2341 cgtctcgtgg tgttgaaacg ccgcgtttgg aagccgagct tctgttggcc cacgtgctcg
     2401 gtgccaaccg aatccagctc atcgtcgacc gcgaccggcc cctgaccgca cacgaattgg
     2461 gagcctatcg agccttggta acccgacgtc gaaaccacga accggtcgcg tatcttcgaa
     2521 aagagcgcga gttttatggg cacgacttcg aggtggacgc gcgtgttctg attcctcggc
     2581 cggacaccga aacgctggtg gaggttgcct tgaaacgcag ccaggagcgt tacttgttcg
     2641 ggcgattgct cgacgtgtgt acgggttcgg gcaatgtcgc gctctcgttt gcgaaagagc
     2701 gaccgacatg gcaagtattg gccacggatc tatcgtcgga cgccatcgcg gtggcgtcgc
     2761 gcaatgccgt acggctcggt gttgcctgga atgtatcgtt tttcgctgga gaccttttcg
     2821 accccgtgaa aggtcagcca aggttccatc tcattacggc aaacccgccg tatattccga
     2881 gtgaacaggt ggaccagctt tcacccagca ttcgcaatca tgaaccacgg ttggcgctcg
     2941 atggaggcca agacggtctg gacttgattc gtcggcttat ccacgatgct ccgtcatggt
     3001 tggaacaagg aggcgaattg gcattggaga tggcttgcga ccaaggctcg cgggtggtgg
     3061 agatgatgac ccgcaatggg tggcaagacg tgcaaatcgt acgggatttg ggccaacggg
     3121 atcgggtggt tcgtggaatt cgtccctgac aaaatagaac atccccgaat tcggaccggc
     3181 aacagagcgt aaggatgatc gcgatagcga tgggtgaccg atccgaattc gaggagtatt
     3241 cgcccgcggt cgcgagcccg tcggggaaac gacggtaagc catggtaatc caagcaagtg
     3301 atttcagact aagtaagaaa ggaaccagca cgacgccttg gatgaagtcg gcggctgtgc
     3361 tgtgagcaag aaggcaaagg cggtgccaca aggaaatata tattgatttt gaatagttat
     3421 ggatttgtcg agagacgacg aggcgttcga cccctgacag ctctgtcgcg actgtggttg
     3481 ggaagtcacg ggaaatgtcg ttgaatcagg ggcaatgggg gttacgcagg gcgtaggtga
     3541 cgattctgct ggatgcccag ctactttttt ttgaacagct ttccgaagat gtcgctgacg
     3601 ctgtcttgtg tccaattgat ggattcggat gcaccgggtt tggttttggt gcgttcggaa
     3661 gacgtgggtt tttcgtcggc gagggctttg gctcctcctc gcatggtggc gagacggacc
     3721 tctcggcttg cttcgatatt gcgggggttg agttcaacga cttgtttgaa gtcacgcaat
     3781 gcggaggcgt gctttcccaa ttgtttgttc aacatggccc gatagaaaaa tgcctgttca
     3841 caacgggcgt cgagtttgat ggcttggtcg agcaaacgca gggaggtttc gaagtctttg
     3901 gattgctggg cctctggttg ttgggacaga acccaggcgt aaagcgctag atggtgcgac
     3961 tgttccggat cgccctcgta ggctttttgt gcggagcgaa gggctttacc gaggtcgtgt
     4021 tttcgaaggt agacttccgc tttctgatac tcgatggtgg cttcgatgac ttgctgaact
     4081 ttggcctgtt cgtccttgtt ggttccacct ttttcgagag cctcgacgta ttgggctctt
     4141 ttgtccgggt cgctcagcgt ttggaacgca tcggacattc gtgcgaatac ctttgccgca
     4201 tcatcccgga cctcatggta cgagtcggga agtcggtcgg gatgccactg cttcgccaaa
     4261 gaaaagtatg cggaacgcac cacgtcgggg gaagcgttct gatcgacgcc caaaacctcg
     4321 aaatagcttt ctgcgtcgac ttgggaagcg cgctgttgaa tgtcctgcgg ggaaagcgcc
     4381 atgggtgtaa cggtcgaaga agcggcgact ttcttcaact tgacccgcgc catggggacc
     4441 gcttgtcgcg atgcctgggc ggcttcggaa ccggtgcgac tggagtccgg gggcgcgttg
     4501 ggggcaacca ctgccggttc cgaggcttgt ggtttccgcg ctggtgcgct ggtttgcacc
     4561 ggcaccacca cgattcccct ggtgatcatc agcacgtaca acagaagctt gagctgcctt
     4621 atgggcatga tgttcaggtc gagcacttgc gcgacggaca tgggtcgaag ggcgagcagt
     4681 tcgatcacct ttccttcggg agggccaaac ccaaatcgac gaaaatccgc gttggtgttg
     4741 atgccaatgg gcgccgcggc gagacgggcc agggtcggat cgatgctggg gtcattgggc
     4801 cgcacatgca ttccctc
//
LOCUS       DAOSOR010000468         4789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_17825_length_4789_cov_3.988382, whole genome shotgun sequence.
ACCESSION   DAOSOR010000468 DAOSOR010000000
VERSION     DAOSOR010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17825_length_4789_cov_3.988382"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PK710_19795"
     CDS             complement(<1..322)
                     /locus_tag="PK710_19795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12029.1"
                     /translation="MTPIPDLAKSVPPDVLFLCRRLRDRGWRCWIVGGCVRDLLLGRF
                     VHDWDIASDARPNDIMSLFRKVIPTGLQHGTVTVVLNAQRYEVTTLRGDGAYTDGRHP
                     DQIRF"
     gene            complement(432..2102)
                     /locus_tag="PK710_19800"
     CDS             complement(432..2102)
                     /locus_tag="PK710_19800"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT12030.1"
                     /translation="MSDSSSVPLVNPNPSDPSGVVETVLELEEEEVPLLEPEPSEEPT
                     PAGSRLKSDPYIGSTFDGRYKVESILGEGGMGVVYRGRHKVIDKAVAIKVLRVDLARD
                     AEVSERFLREARATSAIDNPHIIDISDFGTLPDGSTYFVMEYLDGKPLTSVFDDEGHP
                     VPITRLIHIAKQIAVGLGAAHDRQIVHRDLKPDNVFLIRHGDEKDFVKILDFGIAKTA
                     SETAKLTRHGSVFGTPHYMSPEQAAGAAVDHRTDIYSLGVIMYEMASGRLPFDADNFM
                     GILTQHLYQAPVPIRALVPPQDIPAGLEAIILKCLSKKLQLRYQSMQELIADIDKLSE
                     GQAPGAIDEMTSRSGGFNVPADFFKSAMPVAVPATPAHLQKKRPWLLMAGIASLVVAT
                     AAVLGVVLLHDDKGAALSTPVVASDQLPVTPPSTSTTPSPIVSAAPTTRFIDVMIVSN
                     APNAEILLGDKRLGEAPNLVPVPEGQTVQVTVRAAGYASQTVELDGSEKRRNIVLRAQ
                     SLGRSAPKSTETTDRTPHRPPPASTPSPPNPKTPPTMGTGEIINPWAK"
     gene            2635..>4789
                     /locus_tag="PK710_19805"
     CDS             2635..>4789
                     /locus_tag="PK710_19805"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT12031.1"
                     /translation="MSESPLGPVPTQLAHFEIKGRIGFGGMAEVFLADKHGAENTCKR
                     LVVKRILPVYGNNSDFREMFKAEAGVATRLNHPNIVQVYEFQEFAEAGQLLSMEYVEG
                     LDLGKLMAYARIKSTRIPPWVAAYIIAEAAKGLHYAHERKSDDGVALAIVHRDVSPQN
                     ILLSYEGAVKIADFGVATANLFRDKVGVLKGKYGYMSPEQARAEIVDRRTDIYALGVI
                     FYEMLAGRPLRPGLSGDDLLAAVRSGQIVAPSTYVRDIPTELEAIVLRALSARKEERY
                     QTARDMAGAISRAMMQRQQLVEAVTLEGTIEALAPRNITSPDVDRKLAFRQSGGLGDL
                     AAQNTVAAVRSARDGSGISSVGTHDEPDIEAKKTAPNKVTKEVRHVAILTLRLHGLEE
                     LRQLGGAERGIEREIRNMLDSLAFKWGARLTWTSHDHARAIVGLLANPSRATSDAANC
                     AIDIQEFLKASAEEWPVTVQASIGIVRGIASGVRDHQGHLIDHALQNPAEFLASLVGD
                     RAPAGCTWAAGGIYRLVRDEFVWRDALPLHIEDAAARQAPSTMRIHELIRRFSRGERA
                     IRREASPSDLIGRDAEKADLHSALHNATQPAEAGAEHVVVRVVVGEMGIGKSALVRAF
                     LNEVRSDVSRIHLDPSPANSELAFGAVAELVRALSSTSSDMKLEQAQAVLNDLLGMPG
                     GAITYRLAQLVSGEREEHGEDEDAAYVRKIVVSGLR"
BASE COUNT          983 a         1241 c         1509 g         1056 t
ORIGIN      
        1 cgaatcggat ctggtcggga tgtcgaccgt cggtataggc gccgtctccc cgcaaggttg
       61 tcacttcata tctctgggcg ttcaagacca cggtgacggt tccgtgctgc aatcccgtcg
      121 ggatgacttt tcgaaacagc gacatgatat cgttgggacg cgcatccgaa gcgatatccc
      181 aatcatgcac gaagcggcca agcaacaagt ctcgaacgca gcctccgacg atccagcatc
      241 gccatccccg atcgcgtaaa cggcggcata gaaacagaac gtctggggga acggatttcg
      301 ccaaatcggg gataggggtc atggtcgatg gggcatggag agggaaggcc ccctccgcgg
      361 cggtggtatc gatgggattg tggcatgctt ggcgccgcga aaccttgtca ttcgtaaggc
      421 tgatcgtcca gtcacttggc ccaaggattg atgatctcac cggtgcccat ggtaggaggc
      481 gttttggggt tgggggggga aggggtcgaa gccggtggtg gtcgatgggg agtacgatcc
      541 gtggtttccg tggattttgg cgcagagcgt ccgagggact gtgcgcgcaa gacgatgttt
      601 ctgcgctttt cggagccatc gagttcgacc gtttgggagg catatccggc ggcacgcacg
      661 gtgacttgga ccgtttgccc ttcggggacg ggaaccaggt tgggggcttc tcctaatcgc
      721 ttatctccca acaaaatctc ggcgttgggg gcattgctca cgatcatgac gtcgatgaac
      781 cgtgttgtgg gagcggcgga aacgatggga gagggggtgg tcgaggttga tgggggtgtg
      841 acgggcagtt gatcggaagc cacgacgggt gtggacaggg cggcgccctt atcgtcgtgc
      901 aatagcacga caccaagcac cgcggcggtg gctaccacca aacttgcgat tcctgccatc
      961 agaagccaag gccgtttttt ctgaaggtga gcgggggtcg cgggcaccgc taccggcatg
     1021 gcggacttga aaaagtcggc gggtacgttg aagccaccgg agcgagacgt catttcgtca
     1081 atggcaccgg gggcttgccc ttcgctcagc ttgtcgatat ccgcgatgag ttcctgcatg
     1141 gattgatagc gaagctggag ttttttcgac aagcacttca ggatgatggc ttccagaccg
     1201 gcaggaatgt cctgcggcgg aacgagagcc cgaatcggca cgggagcttg atacaggtgt
     1261 tgggtcagga tgcccatgaa gttgtcggca tcgaaaggca gcctcccgct cgccatttcg
     1321 tacatgataa cgcccaagga atagatgtcc gttcgatggt cgacggcagc ccctgctgct
     1381 tgctcgggag acatatagtg gggggttccg aaaaccgagc cgtgtcgcgt gagcttggct
     1441 gtttccgaag ctgttttcgc tatgccgaaa tccagaattt tgacgaagtc tttctcgtcg
     1501 ccgtgccgaa tcagaaagac gttgtctggt ttcaaatccc gatgaacgat ctggcggtca
     1561 tgggccgccc cgagaccgac tgcgatttgt ttggctatgt ggatcaaacg agtgatgggc
     1621 accggatggc cctcgtcatc gaacaccgac gtgagcggct tgccgtcgag atactccatg
     1681 acgaaatagg tggatccatc gggtaacgtg ccgaagtcgc tgatgtcgat gatatgaggg
     1741 ttgtcgatgg ctgacgttgc gcgtgcttcg cgcaaaaacc tttccgatac ctccgcatcc
     1801 ctcgctagat cgacacgaag caccttgatc gcaaccgctt tgtcgatgac tttgtgccga
     1861 ccccgataca cgacccccat gccgccctca ccgagaatac tctccacctt gtagcggccg
     1921 tcgaaggtgg aaccgatgta gggatcggac ttgagacggc taccagccgg cgtgggctct
     1981 tcggatggtt cgggttccag aagcggtact tcttcctctt ccaattccaa cactgtctcg
     2041 acaacaccgg atgggtccga gggattggga ttcaccaggg gtacgcttga ggagtccgac
     2101 atcagcatca ccgacggttg cgcctcatga ttttttcgga gccgacgcgg cgcatctcta
     2161 agatgaacaa gagatgggcg atcgcgcaag cttttgactt atggttcttc tctcagccct
     2221 tgggaacatc gcatctgtta tttcgcattt tcgccgttga tgaaatatat gcagtgagaa
     2281 ttgcggaaat cgaacaaaat gctcgagtcg attcctccaa cccctcgcgc atcgccctct
     2341 gcccaaaatc tacatttcat atgtaaattc actgtgttgt attaattgag ccccttggtg
     2401 ggggaagacg aaatgcgcgg cgcattcatc gccatctcgg agacacggac gggatgtcgt
     2461 ttggggcatc gtggaaccga gcgtaggcgt tttcgtattt tgttcagttt tttgcaggag
     2521 cgtgccttgc cggtctgacg tgagaggtgt ctggacccgg gtcaggggtg ggctgagcca
     2581 cacaagactg gctcagcctg gtcggcttgg gctaacgtaa gggtgaagta caccatgtcc
     2641 gaatcgccgc tggggcctgt gcctacacaa ctagcccatt tcgaaatcaa aggcaggatt
     2701 gggtttggtg ggatggcgga ggtatttctt gcggataagc acggtgcgga aaacacgtgt
     2761 aagaggttgg tggtcaagcg gatcctgccc gtctatggca acaacagcga ttttcgggag
     2821 atgttcaagg ccgaagcggg ggtagccacc cgtttgaatc atcccaacat cgttcaggtt
     2881 tacgagtttc aagaattcgc ggaagcgggg caactgctat cgatggaata tgtcgagggc
     2941 ttggacctcg gaaagctcat ggcctatgcg cgtatcaagt ccacgcgtat tccgccgtgg
     3001 gttgcggcgt atatcatcgc ggaggcggcc aaagggcttc attacgccca cgagcgaaaa
     3061 agcgatgatg gtgtcgcgct cgccatcgtc caccgtgatg tatcccccca aaatatttta
     3121 ttatcctatg aaggcgcggt aaaaattgcg gattttggtg tcgcgacagc gaatctattt
     3181 cgcgacaagg tgggagtgct caagggcaag tacggatata tgtcgcccga gcaagcacgg
     3241 gcggaaatcg tcgatcgtcg caccgatatt tatgcattag gggtcatttt ttatgaaatg
     3301 ctcgcgggtc gtcccctgcg cccaggatta tcgggcgacg atcttctagc ggcggttcgg
     3361 tcagggcaga tcgtggctcc gtcgacgtac gttcgcgaca ttcccaccga actcgaggcg
     3421 atcgtgttgc gtgcgttatc cgcgcgcaaa gaggagcgat accaaacggc ccgcgatatg
     3481 gcgggagcca tttctcgcgc catgatgcag cgtcagcaat tggtggaagc cgtcacgctc
     3541 gaaggcacga tcgaggctct cgcacccaga aatattactt cccctgacgt tgatcggaag
     3601 cttgcgtttc ggcagtcggg cggcctcgga gaccttgcgg cccaaaacac ggttgcggcg
     3661 gtgagaagtg ctcgcgatgg ctcggggatt tcgtcggtgg gcacccacga tgagcctgat
     3721 atcgaagcga aaaaaacggc tccgaacaaa gtgacgaaag aagtacgtca tgtggcgata
     3781 ttgacgttgc ggttgcatgg tttggaagag cttcgtcagc tcgggggcgc cgaacggggc
     3841 atcgagcgtg agatccgaaa catgctcgac agccttgcat tcaagtgggg cgcgcgcctc
     3901 acctggactt ctcatgatca tgcccgggcc atcgtcggtc tgttggcaaa cccgagtcga
     3961 gccacctctg atgccgcgaa ttgtgccatt gacatccagg agtttctgaa agcttcggcg
     4021 gaggaatggc cggtgaccgt gcaagcctcc atcggcattg tgcgtggaat tgcctcgggg
     4081 gtgcgggatc atcaagggca tttgatcgac catgcccttc agaatccggc ggaatttttg
     4141 gcctcgttgg taggggatag ggccccggcg ggatgcacat gggcagcggg agggatttat
     4201 cgactcgttc gcgatgagtt tgtgtggcgc gacgctttgc cgttgcatat cgaggatgct
     4261 gccgcgcgcc aagctccttc cacgatgcgc atacacgagc ttattcgtcg tttctcccgg
     4321 ggcgagcgag ccattcgccg tgaggcgagt ccttcggatc tcattgggcg tgatgcggaa
     4381 aaagcggacc tccactccgc tctgcacaat gcaacgcagc cagcggaagc gggggcggaa
     4441 cacgtggtcg tacgcgtcgt cgtgggggag atgggcattg gaaaaagcgc tctcgttcgc
     4501 gctttcctca atgaagtccg atcggatgta tcgcgcatcc atctggaccc ttcccctgcc
     4561 aacagcgaac tggcgtttgg tgccgtggcc gaactcgtcc gcgccctcag ctcgacttcc
     4621 tcggacatga aattggagca agcgcaggcg gtactcaacg accttttggg catgccgggc
     4681 ggggcgatca cgtatcgtct tgcgcagttg gtcagtgggg aacgggaaga acatggggaa
     4741 gacgaagacg cggcgtacgt tcggaaaatc gttgtgtcgg ggctgcgtc
//
LOCUS       DAOSOR010000469         4766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_17928_length_4766_cov_4.132456, whole genome shotgun sequence.
ACCESSION   DAOSOR010000469 DAOSOR010000000
VERSION     DAOSOR010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17928_length_4766_cov_4.132456"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            410..2152
                     /locus_tag="PK710_19810"
     CDS             410..2152
                     /locus_tag="PK710_19810"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT12032.1"
                     /translation="MGSQSDNAVRLPANIGPGTVLSKRFKLIKPIGSGGMAIVWRAEH
                     LVLGIPVALKMLRSVDAHSQVARARFEAEARVVAALKHPNVVHILEYGVHGEVPYFAM
                     ELLEGETLAKRLQRRKTLDAVSVVWIVEQIARPVDKAHAKGIIHRDIKPDNIFLHRTD
                     DGEVPKLVDFGIAKLIQSDDKQALTRTDAVIGTTHYLSPERARGKKPIDAKVDLWALA
                     VVVYECVVGRRPFDEHADDLLGLLKAIAEVDYPPPSTLRPIPPEFDAWTDQALNPNPN
                     RRFENAAEMAAALREALLPGTVSSLTGMAFEALEPEMRPPSMSSVTETGISVDCSQAS
                     STEQSLDHDPSSPEYSPLPEAFATQPQLRRYDPGGESHSVEVEPATNPVNFAELDGAV
                     PTVRQEPHQPKSLSISEDTDEPTRIMRDVAVALRSDDWNDTTTNPVSGSASTVDGSIV
                     SIRAPGTEWSEARPRRRWRLYALAVLTMFVAAGVGASSVWLAAPKLLTASVQELDPEA
                     SLAVTEPVVTAAPASVSSPPALSVVPPVLDVERLPITDTEGSSSTPVPSQAPPRRYPK
                     PIDKRRKPHEVFGL"
     gene            2191..3132
                     /locus_tag="PK710_19815"
     CDS             2191..3132
                     /locus_tag="PK710_19815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12033.1"
                     /translation="MHCVFATVLAFSIVCAPIHAQTPSRTHREAAIALGEEGLALLDQ
                     GEYAAALRKFDAADELIPAPTFGVRAAMCLEKMGHWVEAIHRYEQVVAIRVDPSWPEI
                     HVKAQQEARVRAEALRHRVARLEVSVVGPPDAQYSLKLDAAPVDLDSTSTVLLNPGEH
                     VLELIHGTTSDLRRFQVAEAEHRTERFTLRPPTDFPPSSSSLHGTVGWVSIALGVTGV
                     LVGSVTGYVAYEKKNDLEVRCPKRICPPTAWEDNQSYDRWRTASTAGFVGGTIALGLG
                     TLLLLTEPSTEPAPTATGRFSLKPWFGATTAGFGGEF"
     gene            3132..3875
                     /locus_tag="PK710_19820"
     CDS             3132..3875
                     /locus_tag="PK710_19820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12034.1"
                     /translation="MWRRLLPPLACMLAPIGVIGCVLPDFDNGVGPSPHTGGGTQQDV
                     VSEWPEFDSGPDSEEDIAMEAEPDVDIDASFCETHPEGTVCGESGICDEKVCVAGVCE
                     LVLIPDGVECEQAPNACWKPGVCESGQCTFPVAFPDGYNWKPGDNLARCCKGEPRMVN
                     TNDDCGVCGIKCDTSDGQSCKRNPANNRYYCEGCQASAKCWSGCCSESFMPIRRCAAS
                     DCSTGACVGCPQGTSCVTSSVASNVCTYD"
     gene            complement(4107..>4766)
                     /locus_tag="PK710_19825"
     CDS             complement(4107..>4766)
                     /locus_tag="PK710_19825"
                     /inference="COORDINATES: protein motif:HMM:NF014213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12035.1"
                     /translation="FAPRIDNLASCHAALSSLVEAASQVPMLEPTRVVALWDNEECGS
                     TSMQGARGTLLRGILTRVISARGGNIAEDVDRSLSRSLLVSVDMAHAIHPNYPDRHDR
                     EHAPVLGKGPVIKSNANQSYATDAVSSARFVRACRSAGFTPQRFVVRSDLPCGSTIGP
                     IVAALTGIKTVDVGNPMLSMHSIREMAACADHAMLIDAIGHLLRLDEKKRKGRKIRLV
                     S"
BASE COUNT          962 a         1287 c         1431 g         1086 t
ORIGIN      
        1 gatgatcacg gtcttttaca agatctatct cggggttcgc gaaagcatcg tctaacctcg
       61 ccctcttcat gacaccacca cggaagcaag gaagaaacga cgggtggggg cgtttctaac
      121 ggttcggtgt gtggttgacg tgatgcgtaa tgtcgacaat tcgtggaggg tggtggggtc
      181 gaagatcggg gatacacacc cgaattcgag cgcacccgtt cgttggcgga gggggggcgg
      241 gggggcatga cccggttttc aagcctactt gggcaatgga tggggcggag gctttggaga
      301 ttgggaaagg taccgtctcg gtgtcgacgg gagacggcac gggccttggc cgattcaggg
      361 gcgccgttcc cgtagcgcgt tgcgctccgt tctggcaagg ttgttgctga tgggatccca
      421 atcggacaat gcggttcgtc ttccggccaa catcgggccg ggaaccgttt tgtccaaacg
      481 attcaaactc atcaaaccga ttggttcggg gggcatggcc atcgtttggc gggcggagca
      541 cctcgtattg gggattcctg tcgccttgaa gatgctgagg tcggtggatg ctcattcgca
      601 agtggctcgc gcgcgattcg aggcggaagc gcgggtggtt gctgctctca agcatccaaa
      661 tgtggtccat atcctcgaat acggtgtgca tggcgaggtt ccgtatttcg cgatggaact
      721 gctcgaaggc gaaaccctcg cgaaaagact gcaacgacgc aagaccctgg atgccgtctc
      781 cgtcgtttgg attgtggagc aaatagctcg ccccgtggac aaggcgcatg ccaagggcat
      841 cattcacagg gacatcaaac cggacaacat tttcttgcac agaaccgatg atggagaggt
      901 tcccaaactt gttgacttcg gaattgcgaa gttgattcag agtgatgaca agcaagcgtt
      961 gacgcgaacg gatgcggtca tcggcaccac ccattatttg agtccggagc gtgcacgggg
     1021 gaaaaaaccg atcgatgcca aggtggacct atgggctttg gcggtcgttg tgtacgaatg
     1081 cgtcgtgggt aggcggcctt tcgatgaaca cgcggatgat ctgctcgggc tgctcaaagc
     1141 cattgccgaa gtggattatc ctcccccatc caccttgcgt cccattcccc cggagtttga
     1201 tgcttggacc gatcaagctt tgaatccaaa tccgaatcgc cgttttgaaa acgctgccga
     1261 aatggcagcc gccttgcgag aagccctcct tcctggaacc gtatcgtcgc tcacgggcat
     1321 ggcgttcgag gctcttgaac cggaaatgcg gccaccttcg atgtcatccg tgacggagac
     1381 cggcatttcg gtcgactgca gtcaggccag ttccacagag caatccctcg atcacgatcc
     1441 ttcctcgccc gagtattctc cactgccgga ggcctttgcc acccaacctc agcttcgacg
     1501 ttacgatccg gggggagagt ctcactctgt cgaggtagaa ccagccacca atcctgtcaa
     1561 ttttgctgaa ctcgatggtg cggtacccac cgttcggcag gagccgcacc agcccaaatc
     1621 gctttctatt tcggaagata ccgacgagcc gacgcggatt atgcgtgatg ttgccgtggc
     1681 gttacgttct gacgattgga acgatacgac aacgaaccct gtgtcgggct cggcatcgac
     1741 ggtggatggt tccatcgttt ccattcgggc tccggggacg gaatggtccg aggcgcggcc
     1801 tcgtcggcgg tggcgattgt acgcgcttgc ggtcttgacc atgtttgttg ctgccggtgt
     1861 cggggcatcg tcggtttggc tggccgctcc caagctcctc acggcttctg ttcaagaatt
     1921 ggacccggag gcttcgttag cggtgactga gccagtagtc accgcggctc ccgcgagtgt
     1981 atcatcgcct ccggcattgt cggttgtacc gccggtactg gatgtggaac gtttaccgat
     2041 aacggatacg gaagggtcat cctcaacgcc tgtgccatcg caagcgcccc ctcgccgtta
     2101 tcctaaacct attgataagc gacgaaaacc gcacgaggtg tttggacttt gaccgatatg
     2161 ggaagtcgag tttcgcgaag catatctcgc atgcactgtg tgtttgcgac tgtgcttgcg
     2221 ttctcgattg tctgcgctcc catccacgcg cagacgcctt cccgaacgca tagggaggcc
     2281 gcgatcgcgc ttggtgagga agggttggcg ttgctcgatc aaggggagta tgcggcggcg
     2341 ttacgcaaat tcgatgctgc cgatgagttg atccccgcgc ccacgtttgg cgttcgagcg
     2401 gcgatgtgtc tcgaaaaaat gggacactgg gtcgaggcaa tccatcggta tgagcaggtc
     2461 gtagccatcc gcgtggatcc ttcgtggcct gaaatccatg tcaaagcaca acaggaagcg
     2521 cgtgttcgag cagaagcgtt gcgacatcgt gtggctcgac tcgaggtctc tgtcgtggga
     2581 ccccctgatg cacagtattc cctcaaactc gatgcggctc ccgtcgactt ggattccact
     2641 tccaccgtct tgctgaaccc tggtgagcac gtgctcgaac tcatccatgg aaccacttct
     2701 gacttgcgac gttttcaagt ggccgaagca gagcacagaa cggaacgatt caccctgcgt
     2761 ccccccacgg atttcccacc atcatcatca tcgctgcatg gaaccgtcgg ttgggtcagt
     2821 atagcgcttg gagtaacggg tgtgttggtt ggttccgtga cgggatatgt ggcgtacgag
     2881 aaaaaaaacg atctcgaggt tcgatgtccc aaacggattt gtcctcccac ggcgtgggag
     2941 gacaaccaat cctatgaccg ttggcgcacc gcatcgacag ccggttttgt gggaggaacg
     3001 atcgctttgg gattgggaac gctccttttg cttaccgagc cttcgacgga acccgccccg
     3061 accgcgaccg gtcgcttttc tctcaagccg tggtttggtg ctacgaccgc gggattcgga
     3121 ggagagttct gatgtggcgt cggctccttc cgcctcttgc ctgcatgctg gctccgatcg
     3181 gtgtcatcgg ctgtgtgctt ccggacttcg acaatggtgt tggaccgtca ccccatacgg
     3241 gcgggggtac gcaacaagat gtggtatccg aatggccgga gttcgattcc ggaccggatt
     3301 ccgaagagga tatcgccatg gaggccgagc cggatgtcga tatcgatgca tccttttgcg
     3361 aaactcatcc agaaggaacg gtttgtggcg aatccggtat ttgtgatgag aaggtctgtg
     3421 tcgctggcgt atgcgaacta gttctcatcc ccgatggcgt agaatgcgaa caagctccca
     3481 atgcgtgctg gaagccggga gtttgtgaaa gcgggcagtg tacgttcccc gtggctttcc
     3541 ccgatggtta caattggaaa ccgggcgata atctcgctcg gtgttgcaag ggagaaccac
     3601 gcatggtcaa caccaacgac gactgtggcg tgtgtggaat caagtgcgat acgtccgatg
     3661 ggcaaagctg taaacgaaat cccgcgaaca accgttatta ttgtgaagga tgccaagcga
     3721 gcgccaagtg ttggtcaggt tgttgttccg agtccttcat gccaattagg cgttgtgccg
     3781 cgagcgattg ctcgacgggt gcgtgcgtcg gatgcccaca aggaacttcc tgcgtaacct
     3841 cttctgtcgc ttcgaacgta tgtacgtacg attgaccatg ggccggcaaa gcgccgaatg
     3901 gaaaaacttg ttccgcacgt tcctacgatc tccggactcc cactcaaatt cgtgatgcgc
     3961 agccgaagca tccagccttc gagggggcct cgaacggtgt ggtctgcacc accggctagg
     4021 tataaagatc tataatttcg tctagttgcg atgggttggg ttgcggtggt ggcgaaggtg
     4081 tcgattcgtt tcaaggcttg tgaggttcaa gaaaccaatc gaatttttct tccctttcgt
     4141 ttcttttcat ccagtcgaag caagtgaccg atcgcgtcga tcagcatggc gtgatccgcg
     4201 caggccgcca tctctcgaat cgaatgcata ctcagcatgg gattgcctac atccacggtt
     4261 ttgatgcctg taagcgctgc aacgatcgga ccgatggtcg agccacaagg caagtccgaa
     4321 cgtacgacga accgttgtgg tgtgaaccct gcgcttcggc acgcgcgaac gaaccgtgcc
     4381 gacgatacgg cgtcggtggc atacgattgg ttggcgttgg atttgatgac cggtcctttg
     4441 ccgagcacag gggcgtgttc gcgatcgtgt cggtccgggt aattgggatg aatcgcatgg
     4501 gccatatcga cggaaacgag caacgatctc gaaaggctac gatccacgtc ctccgcgata
     4561 ttccctcctc gcgccgatat cacacgcgtc agtatcccgc gcagcagggt gcctcgagcc
     4621 ccttgcatgc tcgtgctccc gcattcttcg ttatcccaca gagcgaccac tcgtgtcggt
     4681 tcaagcatgg ggacctgcga tgcggcttcg acgagcgatg acaaggccgc atgacatgaa
     4741 gccaaattat cgatacgtgg cgcgaa
//
LOCUS       DAOSOR010000470         4761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_17951_length_4761_cov_3.993625, whole genome shotgun sequence.
ACCESSION   DAOSOR010000470 DAOSOR010000000
VERSION     DAOSOR010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17951_length_4761_cov_3.993625"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..559
                     /locus_tag="PK710_19830"
     CDS             245..559
                     /locus_tag="PK710_19830"
                     /inference="COORDINATES: protein motif:HMM:NF021188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HOT12036.1"
                     /translation="MSFRIDHAHSLPLEQAVERIRALGDYLNNKHGIGVTWTGPHTAH
                     VKGNFMVVKIDATVSVETSKVVFDGKDPGMLWRGKAREYLERKMRKYLDPSCPVSELP
                     RS"
     gene            complement(596..1327)
                     /locus_tag="PK710_19835"
     CDS             complement(596..1327)
                     /locus_tag="PK710_19835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009566864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOT12037.1"
                     /translation="MLENREGKRIPNVSFKVIENGTWKTITTDDLFKGKKVVVFSLPG
                     AFTPTCSSSHLPRFNELANTFKKNGIDDIVCMSVNDTFVMNEWKKDQEAENVTLIPDG
                     NGEFTEGMGMLVDKSNLGFGKRSWRYSMLVDDGVVKKMFIEPQVDGDPFEVSDADTML
                     GYVAPNAEKPKFFTLFTKRGCPHCARAKKLLQDKGMLYEEIELSRSVTSRSLRAVSGG
                     ATTPQVFLGGKLIGTADDLEAYFKG"
     gene            1723..4302
                     /gene="bamA"
                     /locus_tag="PK710_19840"
     CDS             1723..4302
                     /gene="bamA"
                     /locus_tag="PK710_19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOT12038.1"
                     /translation="MAMLALIGAYANAQPADSRPESSSSTPSSEAEDKPKDTKRPASS
                     KDPYADTALRPAPQLPPTEAELARGLPIMEIQVAGNQRVAAEDIMTYLRTRTGQLFQP
                     EELTRDVRELWDSGFFDDIIVDMNRKDDGVRIRFVVRERSSVKTVTFEGNLALDADKL
                     QEAIEVKNDTILSYPAIRRAIQKIRDMYAEEGYFLAEVEHEIIPQRDNEVVIAFKIKE
                     HDQVSVRRVTIIGNENVSEAELREVMYTGQGGFLSFGSGGPFRQDAFERDVLMLNALY
                     YDRGYLSVQVAAPRVDLTPDRTGIELTLSLVEGPQYKIRRLRVYEVDADGKEVEPLGG
                     RRALREMVRAKPGDVFKRAELALDLQRVRTLYRDAGYANVQADPETNLDPEHELVDVI
                     VPIRRGPLVYIDRINIRGNTKTRDKVMRREMEIQEGDLFSETKLDESRSRIIALGYFE
                     RVDFSTEQGAEDDKIQVNIEIVERPTGTFQVGAGFSSIESFIATAQVQQANLFGTGQT
                     LALQAQVSGLRQLVNIHFYEPYFLDSDFSFSTELYDQLRIYDDFSQTSLGGAATFGYA
                     LVRPHTFGSITYTGEYDEVSTVSRSTFLGTSSAVSVFRRLPLANLFRDGFTSSVRPAI
                     TYDTRDNRLFPSSGMYLRGSVEWATPHLGSDNEFLRYSGTGRFYYSLGGGFVAKLNTS
                     LGYVTSPSPEGVPIFARFFLGGIFDLRGFRLRTVGPRLPLASHLDPNAAPISNGANIG
                     GNLMYYQNLELEIPIIEKVGVRGVLFTDAGNAWNTESLYCNAARGQSQQSNGSMEALG
                     VYSVNDPCFSLDSLSSLRTSWGLGLRWFSPMGPLRFEWGFPFKRLAYEESSVFEFTIG
                     NFF"
     gene            4420..>4761
                     /locus_tag="PK710_19845"
     CDS             4420..>4761
                     /locus_tag="PK710_19845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12039.1"
                     /translation="MIRPLASPTPNPFSYALTTLAIASLAACSSWLGFDDVEFESAAG
                     NGGSGGLGNTGGVAGQSGSGGSAGATGGEGGNGGTGATAGIAGSAGDRGVGGSATGGA
                     AGTAGSAGGPAG"
BASE COUNT         1023 a         1453 c         1280 g         1005 t
ORIGIN      
        1 caaaacgcca acgatggctt cacccccaaa tatccaattc caccagctcg ataccgcagc
       61 gcggccgggt tcacgagaac cgaggctacc gtcgagagcc agccaccggg cggaagggca
      121 acaagggcaa gctccaacga cgacggagcg tggctggcaa gcaatacctt acgccaaagt
      181 tagaaacgag atgggcttgc cctggctgga cgtctcacaa gggcggtact atctgttcgc
      241 tcccatgagt tttcgtatcg accacgccca ttctcttccc cttgaacaag ccgtggaacg
      301 cattcgcgcc ctgggggact acctcaataa caagcacgga attggtgtga cgtggaccgg
      361 cccccacacc gctcacgtca aaggtaattt catggtcgtc aagatcgacg cgaccgtgag
      421 cgtcgaaaca tcgaaagtgg tgttcgatgg caaagatccg gggatgcttt ggcgagggaa
      481 agcacgcgag tatctggagc gaaagatgag aaaatacttg gaccccagtt gcccggtgtc
      541 cgaattgccg cgatcgtgaa taccgcgtga gggcagcccg tgaataccga cgggatcagc
      601 ctttgaaata ggcttcgaga tcgtcagcgg tcccgatcag ctttccgccc aaaaatacct
      661 gcggcgtggt cgcgccaccg gacaccgctc gaagggaacg tgaggtaacg ctgcgactca
      721 attcgatttc ctcgtagagc atgcccttgt cttgaagaag ctttttcgcg cgcgcgcaat
      781 gggggcaacc gcgtttcgta aataacgtga aaaacttcgg tttttccgcg tttggggcca
      841 cgtagcccag catcgtatcg gcgtccgata cctcgaaagg atcgccgtcg acttgcggtt
      901 cgatgaacat cttcttcacc actccatcgt cgaccaacat cgagtaacgc caggaacgct
      961 tgccaaagcc gaggttcgac ttgtccacca gcatccccat cccctccgta aactcgccat
     1021 tgccatcggg aatgagtgtc acattttcgg cttcctgatc ctttttccac tcattcatca
     1081 cgaaagtgtc gttgacggac atacatacga tgtcgtcgat gccattcttt ttgaaggtat
     1141 tcgccaactc attgaagcga ggaagatgag aggaggagca ggttggggtg aaagcaccag
     1201 gaagtgagaa aacgacaacc ttttttcctt tgaatagatc gtcggtggtg atggttttcc
     1261 acgtgccgtt ttcgatgacc ttgaagctaa cgttgggaat tcgtttgcct tctcgattct
     1321 ctagcatgag agtctccttg gtctctcgcg gggcgagagg gattgggtat cgtaattcca
     1381 ctcaggtcgc tgggcgcctt ggaggggaac ttggcaacgt tcgatgctgg ctggtgccga
     1441 ggcgttgcgg ctaacccgtc cggaattcgg atggctaacg accgctgcga cccgacatcg
     1501 tcatgccgcg gaaatatggc ttgctcgaca acgcagggaa caggccaagg tcgccccttg
     1561 gcacatttcg tcgccgccac gtactcagca agcttgagac attcggcgca atggggtacg
     1621 agcggaccgc tctcgtatgt gcatctctct taccgcgcca tggtggaccc cgtgaaccca
     1681 atcccttccc gccgcgttcg ggcccgggtt gtgttcgcga tcatggctat gttggccctc
     1741 atcggcgctt acgcaaacgc tcaacccgcg gattcccgac cggaatcatc ctcctcgacg
     1801 ccttcttcgg aagcggaaga caagcccaaa gacaccaaac gacccgcctc ttccaaagat
     1861 ccgtatgcgg ataccgcgct tcgccccgcc cctcaacttc ctcccacgga agcagagctg
     1921 gctcgcggct tacccatcat ggaaatccaa gtcgcgggca atcaacgggt ggccgcggaa
     1981 gacatcatga cctatctgcg cacccgcacc ggtcagctct tccaacccga agaactcacc
     2041 cgcgacgtac gcgaactctg ggattccgga ttcttcgacg acatcatcgt cgacatgaat
     2101 cgcaaagacg atggcgtgcg tattcgcttc gtcgtccgtg agcgatccag tgtaaaaacc
     2161 gtcaccttcg aaggcaatct cgccctcgat gcggacaaac tccaggaagc gatcgaagtc
     2221 aaaaacgata ccattttgag ctatccggcc atccgccgcg cgatccaaaa aatccgtgat
     2281 atgtacgccg aggaaggcta ttttctcgcg gaagtcgagc acgaaatcat cccgcaacgc
     2341 gataacgaag tcgtcattgc cttcaaaatc aaagaacacg accaggtatc cgtgcgccgc
     2401 gtcaccatca ttggcaatga gaatgtgtcg gaagcggaat tgcgcgaggt gatgtacacc
     2461 ggtcaaggcg gctttctgtc cttcggttcg ggcggtccgt tccgccaaga cgctttcgag
     2521 cgtgatgttc tcatgctcaa cgcgctctat tatgatcgag gctacctttc agtacaggtc
     2581 gccgcgcctc gcgtggactt gactcccgac cgcacgggaa tcgaattgac tcttagcctc
     2641 gtggaaggtc cacaatacaa aattcggcgc ctgcgcgtgt atgaggtgga tgcggacggc
     2701 aaggaagtcg aacctctcgg tggccgccgt gccttgcgcg agatggtaag ggccaagccg
     2761 ggtgacgtct tcaaacgcgc cgaactcgcg ctggaccttc aacgcgttcg caccctgtac
     2821 agagatgccg gttacgccaa tgtgcaggcc gatccggaaa cgaacctcga ccccgagcac
     2881 gaattggtgg acgtgatcgt ccccattcgc agggggccgc tcgtgtatat cgaccgaatc
     2941 aacattcgtg gaaacaccaa aacccgcgac aaagtcatgc gtcgcgaaat ggaaatccaa
     3001 gaaggtgacc tgtttagcga aaccaaactc gacgaatctc ggagccgtat cattgctctc
     3061 ggatacttcg aacgagtgga cttctcgacg gagcaaggcg cggaagacga taaaattcag
     3121 gtcaatatag aaatcgtcga gcgccccacc ggtacgttcc aagtaggcgc cggattctcc
     3181 tccatcgaaa gtttcatcgc caccgcgcaa gtgcagcagg ccaacctatt cggaaccggt
     3241 caaaccttgg cgctgcaagc acaggtcagt ggacttcgcc agctcgtcaa tatccacttc
     3301 tacgaaccct actttctgga tagcgacttc tcgttctcca ccgaacttta cgaccaactt
     3361 cgaatctacg acgacttctc ccaaacctcc ctcggtggcg cggccacatt cggttatgcc
     3421 ctcgtgcgcc cccatacctt cggctccatc acctataccg gcgagtatga cgaagtctcg
     3481 accgtttcac ggtccacctt cctgggcacg tcgtcggccg tctccgtatt tcgacgcctt
     3541 cctctcgcga atcttttccg cgatggattc acctccagcg ttcgccccgc catcacctac
     3601 gatacgcggg acaatcgtct cttcccttcc agcggtatgt acttgcgagg ttccgtggaa
     3661 tgggccaccc cccatttggg atcggacaat gagttccttc gctacagcgg caccggacgc
     3721 ttctactatt cgctcggcgg tggcttcgtg gccaaactca atacttccct cggctacgtg
     3781 acgagcccaa gccctgaagg cgtacccatc ttcgcccgct tctttttggg cggtatcttc
     3841 gacttgcgcg gattccgcct ccgtaccgtc ggacctcgcc tcccgctcgc ttcccatctc
     3901 gaccccaatg cggctcccat cagcaacggc gccaacatcg ggggtaactt gatgtactac
     3961 caaaatctcg aactcgagat ccccatcatc gagaaagtag gcgtgcgggg ggtcttgttc
     4021 accgacgccg gcaatgcgtg gaacaccgaa agcttgtact gcaatgccgc gcgagggcag
     4081 agccaacagt ccaatggctc gatggaagcc ttgggggtct actccgtcaa tgacccttgt
     4141 ttttccctgg attccctctc cagtctccgc acgtcctggg gacttggact ccgttggttt
     4201 tctcccatgg gaccgctgcg attcgaatgg ggcttccctt tcaaacgtct cgcttacgaa
     4261 gaatcgagcg tgttcgagtt caccatcggc aacttcttct gagcgccatt gccgccgttt
     4321 ttccaatcca ccatgattcg tcacgcatcg tcggcgatcg cgtgatgacc gaccgagttc
     4381 aggccataat caatcacgcc gcatgcctcg gacatcctca tgattcgacc tttggctagc
     4441 ccaacaccga acccgttctc ttatgcgttg acaacactgg caattgcctc ccttgccgcc
     4501 tgttcctcgt ggcttggctt cgacgacgtg gaatttgaat ccgcggccgg aaatggcggc
     4561 tctggcggcc ttggcaacac agggggcgtg gccggacaaa gcggctcggg ggggagtgcc
     4621 ggtgccactg gtggcgaagg aggcaacggc ggaaccggtg caacagccgg aatcgccgga
     4681 agcgctggcg atcgcggtgt cggcggatcg gctacggggg gagccgcggg caccgccggt
     4741 tccgcgggcg gaccggcagg a
//
LOCUS       DAOSOR010000471         4735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_18074_length_4735_cov_3.490598, whole genome shotgun sequence.
ACCESSION   DAOSOR010000471 DAOSOR010000000
VERSION     DAOSOR010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18074_length_4735_cov_3.490598"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="PK710_19850"
     CDS             <1..917
                     /locus_tag="PK710_19850"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HOT12040.1"
                     /translation="YGVLWKDYDNDGDQDIWVGNYGYSNNLMWNNDGTGTFTDVGVEI
                     GIAKDSVGYQGGNTFGGDFGDIDNDGDLDLFAANIAHPRYQPNSDISMLLINQGPPAY
                     SFVDERASRGLVYDEGDVNGAFADFDHDGALDLAVASLYPGHFSRLYHNDGTGHFTDV
                     SYEANVAVEDAVTVAWADVDHDGDLDLLVVDRSGPRRIHLFINRVGQQKPWIQLDLRG
                     VTTNRGAVGARVTLTAGGITQVREVRAGGGHNVQSDRIVHFSLGEATEITSLQVRWVG
                     GSTETIQGLTPRHRYRVEQGTGMGEIVF"
     gene            complement(950..1111)
                     /locus_tag="PK710_19855"
     CDS             complement(950..1111)
                     /locus_tag="PK710_19855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12041.1"
                     /translation="MLPPFIIEQIRRRENTHRQERVQPQIELPLDDRPARESSPPDEP
                     GVVIIPLWD"
     gene            complement(1282..2055)
                     /locus_tag="PK710_19860"
     CDS             complement(1282..2055)
                     /locus_tag="PK710_19860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT12042.1"
                     /translation="MDIRNQRVCITGASSGIGEACAKAFAAAGCHLCLMARRKGRLED
                     LAVTLQRHHAVDVITFELDVRDRDAVSQLAHRLPSPWQHLDILINNAGLSRGLDPIHR
                     ASIDDWEEMIDTNVKGLLYVTHTLLQAMVQQGHGHVIHIGSIAGHEAYPGGSVYCATK
                     HAVAAINRAMAMDLLGTGVRVSSVDPGMVETEFSLVRFHGDEKRAKGVYQGLQPLSPE
                     DVADAVLFCATRPAHANVREMVLVPSAQASAVHSFRTPS"
     gene            complement(2078..2749)
                     /locus_tag="PK710_19865"
     CDS             complement(2078..2749)
                     /locus_tag="PK710_19865"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HOT12043.1"
                     /translation="MIDIRWSLDHPDLWLGFVQAQNVRWSTTEQALHQSIAETVERMR
                     HQPWPAETTRAAIRDLLRRGGFKPTGRSKPASEYLANTAVDSFPLISNLVDINNLVSL
                     QTGWPCSVLDLDLVGTKEIEVRFGRKQETYVFNSAGQSIDVAGLICLCRLDGDPIANP
                     VKDSMSTKVRESTTRALFVTYTSRRIAQESELQTVLDRVASYLRDHAGASETETQCMT
                     GLTAG"
     gene            complement(2846..3802)
                     /locus_tag="PK710_19870"
     CDS             complement(2846..3802)
                     /locus_tag="PK710_19870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12044.1"
                     /translation="MTSFSRFCCLAIGILAFAGCGSDESENNQNESDAGSDSMNDDVQ
                     ADSPVGDDAKPDTGDSSCTPVNSVSGSVINEQGDAFSNVGVVLCVYQDDGSAKCLNPV
                     KTSASGEFSVTIPAGDGCLESAAYQLKADALSVIPLYCPVDVAAGGAIAMANPNRLVD
                     TPACTRDALGEESKPHEVSAPDDMKMTVIPNGIWLFDFSYDDLGIRRWDAATWGWPCF
                     IDAANAPDDLAVFAPELEVKSDAKDAVHISFPNEAGLSAGTVVDLYGLGGAATSMWDG
                     KEVHEGSWQVIGEAQVSADGSRIETRPGQGLPFATWIGWKKK"
     gene            4202..4393
                     /locus_tag="PK710_19875"
     CDS             4202..4393
                     /locus_tag="PK710_19875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12045.1"
                     /translation="MPKVRKSLTLSMTPGTDSLESTLERHLSTKELSVLLSQGWKMVE
                     TLVPDEQGNVVIVLERDEP"
     gene            complement(4407..>4735)
                     /locus_tag="PK710_19880"
     CDS             complement(4407..>4735)
                     /locus_tag="PK710_19880"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT12046.1"
                     /translation="LSCADALLALQSLHGITFGLWYLSLVTFVQTRADENSRTSLQAI
                     ALSCTGIGSSFASVLGGNLLSHHGARTPFHMAAVCAGIAAFGYAMLAITSRNQNQPHR
                     DISSSE"
BASE COUNT         1043 a         1222 c         1431 g         1039 t
ORIGIN      
        1 gctatggggt gttgtggaag gactacgaca acgatggtga tcaggacatt tgggtcggca
       61 attacgggta tagcaacaac ttgatgtgga acaacgacgg cacggggaca tttacggatg
      121 tgggggtgga gatcggaatc gccaaagact cggtcggata ccaaggaggc aataccttcg
      181 gcggggactt cggggatatc gacaacgatg gggacctgga cttgtttgcc gcgaatatcg
      241 cccaccctcg ttaccaaccg aacagcgaca tcagcatgtt gctcatcaat caaggtccgc
      301 cagcctattc attcgtggat gagcgagcgt cgcgagggct cgtctacgat gagggagatg
      361 tcaacggcgc ttttgcggac ttcgaccacg atggcgcttt ggacttggcc gtcgcatcgc
      421 tgtatccggg ccacttctcc aggctctatc acaacgatgg cacggggcat ttcaccgacg
      481 tgagctatga agccaacgtg gctgtggagg atgccgtgac ggtggcgtgg gcggatgttg
      541 atcacgatgg ggatttggac ttgctggtgg tggaccgttc gggaccgcgc cggattcacc
      601 ttttcatcaa tcgagtcggt cagcagaaac catggattca acttgattta cgcggagtta
      661 caacgaaccg gggagcggtg ggcgctcggg tgacgttgac cgctggaggt atcacacaag
      721 tccgggaggt gcgtgccgga ggcggtcaca acgtgcaaag cgaccgaatc gtccacttca
      781 gcttgggaga agccaccgag attacatccc tgcaagtgag gtgggtgggc ggaagcactg
      841 aaaccattca gggtcttacg ccgaggcatc gatatcgagt ggaacaaggg acgggaatgg
      901 gagagatcgt attttgacac catgggactg cggtagctcg caccgcagtt cagtcccata
      961 gggggatgat gacgacgccc ggctcgtcgg gaggagagct ttctcttgcg ggacggtcat
     1021 cgagcggcaa ttcgatctgg ggttgcacgc gctcctgtcg gtgagtgttt tctctccgac
     1081 ggatttgttc gatgatgaat gggggcagca tggaaacctc ggcttcaagt taagcaaggg
     1141 ttgtgcccat gtctagacct tgaaatgcgg gttttttgaa gatgaccgta tggaaaaaag
     1201 tttcatctct gaaccggatg tcgcggttca gggtgcaaag ctgcatgcac aaaggggggg
     1261 gatatccggg cggtagcttg ctcaagaagg cgttcgaaaa ctgtggactg cagaggcttg
     1321 agcggatggc acgagcacca tttctcgtac attcgcatgc gctgggcggg tagcgcaaaa
     1381 caggacggcg tcggccacat cctctgggga aaggggttgc agcccttggt aaacgccttt
     1441 ggctcgcttt tcgtcgccat gaaagcgcac gagggaaaac tcggtttcca ccattccagg
     1501 gtcgacggaa gacacgcgga cacccgtccc aagcaggtcc atggccatgg ctcgattgat
     1561 agctgccacc gcatgcttcg tcgcgcaata gacgctgcct cccgggtagg cttcatggcc
     1621 cgcgattgac ccgatatgga tgacgtgtcc gtgaccttgt tgcaccatgg cttgaagcag
     1681 tgtgtgggtt acatagagca agcccttcac gttcgtgtcg atcatctctt cccaatcatc
     1741 gatggacgcg cggtggatgg ggtccaggcc ccgggataag cccgcgttat tgatcaaaat
     1801 atcaagatgt tgccatggcg aggggagtcg atgagcaagc tgggataccg catctcgatc
     1861 tcgcacatcc aactcgaagg tgatgacgtc aacggcatgg tgtcgttgca gcgtgacggc
     1921 caaatcctcg agacgtcctt tgcgtcgcgc catgagacaa agatggcatc ctgccgccgc
     1981 aaaagccttt gcgcacgcct ctccgattcc actcgatgca cccgtgatgc atacccgctg
     2041 attacgaata tccatacgtc gctctccttc gtttctttca ccctgccgtc aggccggtca
     2101 tgcattgcgt ttctgtttcc gaagctcccg catgatcgcg aaggtacgaa gccacgcgat
     2161 cgagcaccgt ttgcaactcg gattcttggg ctatccgtcg agatgtatag gtgacaaaca
     2221 aagcacgtgt cgtgctttct cttactttcg tgctcatcga gtccttgacc ggattggcga
     2281 tagggtcgcc atcgaggcgg cacaggcaga tgagcccggc gacatcgatc gattgacctg
     2341 cgctgttgaa cacgtacgtc tcttgctttc ggccaaatcg tacctcgatt tccttggtgc
     2401 caaccaggtc cagatcgagg acggaacacg gccaaccggt ttgtaacgac acgaggttgt
     2461 tgatatctac cagattgctg atgagcggaa acgaatcgac ggcggtgttg gccaagtatt
     2521 cactcgctgg cttgcttcgt cccgtcggct tgaacccacc tcgtcgaagc aaatcgcgga
     2581 tggcagcgcg tgtggtttcc gcaggccaag gctggtgacg cattcgctct acggtctcgg
     2641 cgatgctttg atgaagcgct tgctccgtcg tggaccaacg tacgttttgc gcttgtacga
     2701 aacctaacca aagatccggg tggtccaggg accatcgaat atcgatcaag agattgctcc
     2761 ttaagttttc gccaatgagt tcgaagacag gggaccgtag atcgagcggt gcgcggtttc
     2821 gataggaatg agggggaaat cggttctatt tcttcttcca gccgatccat gtcgcaaagg
     2881 gtagaccttg ccctggacgg gtttcgatac gagaaccgtc ggcactgacc tgtgcttctc
     2941 caatcacttg ccaggagccc tcatgcactt ctttgccatc ccacatcgat gtggcggctc
     3001 cgcctagccc atacaagtcg accacggtcc ccgcggacaa ccccgcctcg ttggggaagg
     3061 agatatgaac cgcgtctttc gcatcggact tcacttccag ctcgggagcg aaaaccgcaa
     3121 gatcgtcggg agcgttggcg gcgtcgatga aacaaggcca gccccacgtt gctgcatccc
     3181 atcgacgaat gccgaggtcg tcgtaggaaa agtcaaatag ccatattcca ttggggataa
     3241 cggtcatttt catgtcatcg ggagccgaaa cctcatgcgg cttcgattct tcacccaggg
     3301 catcgcgagt gcatgcgggc gtatccacga ggcgattggg attcgccatc gcaatggctc
     3361 ccccagcggc gacatccacg ggacagtaca gggggataac cgagagagcg tctgccttga
     3421 gttggtaagc tgccgactcg agacagccgt ctcccgcggg gatggtgacg gaaaattccc
     3481 ctgaagcgct tgttttaacc ggattgaggc acttggcgct tccgtcgtct tggtacacgc
     3541 aaagcacgac acctacgttt gaaaaggcat ctccctgttc gttgatgacc gaaccggaca
     3601 cggaattgac cggggtgcat gaactgtcgc cagtgtccgg cttcgcatcg tcgccaacgg
     3661 gactgtcggc ttgtacgtca tcgttcatgg aatcggagcc cgcatccgat tcgttctggt
     3721 tgttttcgga ttcatcgctt ccgcaaccag cgaaagccaa tatgcctatg gccaagcaac
     3781 aaaagcgaga aaaagacgtc acggtggcct cctatgggtt ggtggctgga acgatgccaa
     3841 ggaagtcgtg cccggctcag tcagacaggc cttctatcta ttgagcacgt tgtcagcaga
     3901 cggcaagacg attggcgacg atgatgtgcg atgagtgtca tcatcgtttg gcttgccatt
     3961 tcgagggaac ctcggaaata aaaaacgaga tgttttgggg ttggattagc caaagcgtag
     4021 cttttggcct gggaagcgtc caggagagcc gctgaaaacc ctggccattg accggaagat
     4081 tcgggagatt ggcaatcgcg gtagctgaac gtccatgatg gtccatcgat gttacgagcg
     4141 ttctttcggt tcattgcgga ttgttgtatg acaaggggga ttgccacgga catgagggtc
     4201 catgccgaag gttcgaaaat cgttgacgtt gtcgatgact ccagggacgg atagccttga
     4261 aagcacgctc gagcgacatt tgagcaccaa agagctgtct gttttgctga gccaaggatg
     4321 gaaaatggtg gagacacttg tgcccgacga acagggaaac gtcgtcatcg tgctcgaacg
     4381 agacgagccc tgaggcgagt gtcgtcctat tcgctggacg agatgtcgcg gtgaggttgg
     4441 ttttggtttc gtgatgtgat ggcaagcatt gcgtatccga aagcggcgat accagcacag
     4501 accgcggcca tgtggaacgg agttcgcgcg ccgtgatggc ttagcaagtt gcctcccagc
     4561 acgcttgcga aagaagaacc aatgccggtg caagacaacg caatggcttg caaagatgtg
     4621 cgactatttt cgtcggcgcg cgtttgcaca aaggtcacga ggctcaagta ccaaagccca
     4681 aaagtgattc cgtgcaggga ttgcagggcg agaagcgcgt cggcacacga taggg
//
LOCUS       DAOSOR010000472         4710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_18209_length_4710_cov_4.797207, whole genome shotgun sequence.
ACCESSION   DAOSOR010000472 DAOSOR010000000
VERSION     DAOSOR010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18209_length_4710_cov_4.797207"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PK710_19885"
     CDS             complement(<1..377)
                     /locus_tag="PK710_19885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12047.1"
                     /translation="MFGISPPRFGVCPTFIGVGALISVMGVDEASAASLSDDVRRVER
                     AWAQVSQAVSVKRPQFLAAGEAFPWLVRVASGAECVSVGVLGARTTDFTVELFHNDLD
                     EMKEGPGADEGVLQSVAGAVYVSR"
     gene            367..1320
                     /locus_tag="PK710_19890"
     CDS             367..1320
                     /locus_tag="PK710_19890"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOT12048.1"
                     /translation="MPNIGPASHKTLSESKRLRTPGWTTLAMVEYALAKRCVVGILAG
                     MRLVVPLLAMLLVACTRTPGPNELTGVGCPKGMVSIPGGSFDMGSNSGDPAESPVHRV
                     TVRGFCMDVTEVTVEAYAACVRGGGCSTLGLQGPSEWWSVACHWGKSGKGNHPINCVD
                     WDQATAYCQWAGKRLPTEEEWEYGARGTDGRTYPWGNGAPGPRLLNACGSECVAWVKK
                     QGHPWKAMHSFDDGWATTAPVGSFPAGDSPFGLKDMAGNVWEWTSSEYSEEYEKNRAN
                     KYRIDRGGGWYYDNPSYVRAAYRNWLLPSLRFINLGFRCAR"
     gene            complement(1356..2228)
                     /locus_tag="PK710_19895"
     CDS             complement(1356..2228)
                     /locus_tag="PK710_19895"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT12049.1"
                     /translation="MPRKRLLVLPSQGQLLISTDLHGNLEDLQALTQRFESLRRCRDD
                     VHWVILGDLVHGPNEEARQTQPELYGYSDQSYLVVETVDVLLRRYPEHVHFVLGNHDH
                     GHVGGPHPSRFHPDEVEHLERSLTEPQRKLLIELFENALLLVVAPCGVLLSHGSPDES
                     LFDAHQVDALSLCYAKNDRHGQWLLSSLLTSYGQTDAVASAMLASVSRGLGFDLRVVV
                     HGHDRDEAGYFTEGQTQVCPVIFGAPRKAKRYLLIDLTGRYESVEDFREGIEVLRLYD
                     EAGSCGKWGNGIGN"
     gene            complement(2431..3351)
                     /locus_tag="PK710_19900"
     CDS             complement(2431..3351)
                     /locus_tag="PK710_19900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12050.1"
                     /translation="MRKDVFGLQISTLCLLPGTLVLTHAPASLAMEGSGAGWLFDGNL
                     GAVRVKSDAYRLAADGAIGYGTGVWGVGVRGSTLAYDEVTSMAHIETAKLDGDAEGWL
                     FLGDGSWTVRPQIRLTVGGASYDSTYTPQAAGAGPWNDQTSWLGRGIAQVGLDWFAGE
                     RFSLAAFAGGGLQVEWYDYESAGAGQASLTDDENTTFRGVARLDMRWKALLDVVSLRL
                     HGDASFFKLTRDRFSVTAGSSMNTVDERVVLSQTEVKTRVYVDIDAAQVWGFRPTVHV
                     GADYFRLSGSEGKLSSWVPLAGVGLLRPWQ"
     gene            3520..>4710
                     /locus_tag="PK710_19905"
     CDS             3520..>4710
                     /locus_tag="PK710_19905"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOT12051.1"
                     /translation="MSSFSKKLQRIQQVGPSSPTPSPSFVPEEETSPVRSSDSLEALR
                     EKIAKIVGRTVAPPPAPSSRPPRTLPFVEHPTPEGSFFQHLMSLDPQHHVGRFPIRLA
                     QSANSATLGLLALDPCLASIDIQRVLYLDTETTGLAGGTGTLPFLVGMGSFSQQGAFV
                     VEQLLLRAPGEEYPLLRRLLDNLQRASAIVTFNGKTFDLPLLRTRLVMNRLPPMPALP
                     HLDLLHVARRIHKTRIGSCHLGAVESRVLGFERVGDVPSDQIAGRYFHFLRTGDEEGL
                     VDVVSHNVWDIVSMAALVGLYGEPLSVLPGEDLAGVAATVARAQEPAQAAEIADIAVE
                     KGGGVPALRLRATLAKARGDRQAALQDFETLEREVDDPGVRLELAKLYEHHLKAYGRA
                     IRVVE"
BASE COUNT          993 a         1396 c         1331 g          990 t
ORIGIN      
        1 cgggagacgt atacggctcc ggccacgctc tggaggacgc cttcatccgc gccggggcct
       61 tccttcatct cgtcgagatc gttatggaat aactccacgg taaaatcggt ggtgcgagcg
      121 ccgagcacgc caacactcac gcattcggca ccggaggcta cacgcacgag ccaggggaaa
      181 gcttcgcccg cggcgagaaa ttgcggtctt ttcaccgata cggcctggga tacttgtgcc
      241 caggctcgtt cgacccgtcg cacatcatcg gaaagggaag cggcggaggc ctcgtcgacc
      301 cccatcaccg aaatgagcgc cccgacgccg atgaacgtgg ggcaaacacc gaatcggggg
      361 ggggaaatgc cgaacatcgg cccagcctcc cacaaaacct tgtcagagag caagcggctt
      421 cgcaccccag gctggacgac actggcgatg gttgagtatg cgttggcaaa acggtgtgtc
      481 gttggtattc ttgccgggat gcggctcgtc gttcccctgc tggcgatgtt gttggtagcg
      541 tgtacccgca cgcctggacc gaatgaattg acgggggtag ggtgtccgaa ggggatggta
      601 tcgattcctg gtggctcttt cgatatgggt tcgaatagtg gggatcccgc ggagagtccc
      661 gtgcatcgtg tgacagtgag ggggttctgt atggatgtca cggaggtgac ggtcgaggct
      721 tatgcggcgt gtgttcgtgg tggtggctgc agtacgttgg gtttgcaagg gcctagcgag
      781 tggtggtcgg tagcgtgcca ttggggcaag agcggaaaag gaaatcatcc gatcaactgt
      841 gtggattggg accaggccac ggcatattgt cagtgggcag gcaagcgatt gcccacggag
      901 gaggagtggg agtatggtgc tcgtggcacg gatggtcgga cgtatccgtg ggggaatggg
      961 gcgccgggac cgcggctttt aaatgcttgt gggagcgagt gtgtggcttg ggtgaagaaa
     1021 caaggccatc cttggaaggc gatgcattct tttgacgatg gttgggcaac gacggcgcca
     1081 gtgggtagtt ttcctgctgg ggacagtcct tttggattga aagacatggc tggcaatgtg
     1141 tgggagtgga catcttctga gtattcggaa gaatatgaaa aaaatcgcgc caacaaatac
     1201 cgtatcgatc gcggtggcgg ctggtactac gacaacccgt cgtacgtccg tgccgcttat
     1261 cgcaattggc tcctgccttc gcttcggttc atcaatctcg gctttcgctg cgcccggtag
     1321 ttcgcgagtt ctcgtttcaa ttcccaattc catttctaat tcccaattcc atttccccat
     1381 tttccacagc tccccgcctc gtcgtataat cgcaatacct cgatgccttc ccgaaaatcc
     1441 tccacgcttt catagcgccc ggtcaggtcg atgagcaaat accgcttcgc ctttcgtggc
     1501 gccccaaata tcacgggaca aacctgggtt tgcccttccg tgaagtatcc cgcttcatcc
     1561 cgatcatggc cgtgtacgac caccctgagg tcgaaaccga gcccccggct cacggaagca
     1621 agcatcgccg aagccacggc atccgtttgc ccatacgaag tcaacaagct gctgagtagc
     1681 cactgaccgt gcctgtcgtt tttggcatag caaagagaaa gagcgtccac ctgatgcgca
     1741 tcaaaaagcg attcatccgg cgaaccgtgg gaaagaagca cgccgcaagg agccaccacc
     1801 aacaataaag cgttttcgaa caactcgata agcagctttc gttgcggctc cgtcaaggac
     1861 cgctccagat gctcgacttc atcggggtga aatcgcgagg gatgggggcc tcccacatgc
     1921 ccatgatcat gattgccgag aacgaaatga acgtgttcgg gatacctccg aagcagcaca
     1981 tccaccgttt ccacaacgag ataagactga tccgaatagc catatagctc cggttgcgtt
     2041 tggcgagctt cctcattcgg gccatgaacg agatcgccta aaatgaccca atgcacgtcg
     2101 tcccggcacc tgcgaaggga ctcgaaacgc tgggtcaaag cctgcaaatc ctcaaggttt
     2161 ccgtgtagat ccgtgctgat cagaagctga ccctgggagg gcagaacgag aaggcgtttt
     2221 cgaggcatga gagatgggaa gtgtgcccaa aagtcccgag atcgatgatg ataccctttc
     2281 ccacgggctt gaccaagtgc aaaaattcac ccttatcgaa gggtagagtc cacccctccg
     2341 aaagccatcg aacggcaatg agtcgcaacc attgtcatca ccccaccccg caacaaccag
     2401 ccctccttgg ctcaggtacg cctgtcaggc tcattgccac ggccgcaata ggcccacccc
     2461 ggccaacggc acccacgacg aaagtttgcc ttccgaaccg gagaggcgga aatagtccgc
     2521 gccgacatgc accgtcggac ggaatcccca cacttgtgct gcgtcgatat ccacgtaaac
     2581 ccgtgtttta acttcggttt gtgatagaac gaccctttcg tcgacggtat tcatggagct
     2641 tccggccgtc acgctgaatc ggtcacgggt gagtttgaaa aaggaagcgt cgccgtgcaa
     2701 ccgcagggag acaacgtcca gcaaagcttt ccatcgcata tcgagccgcg ccacaccgcg
     2761 aaacgtggtg ttttcatcat cggtgaggct tgcctgccca gcccccgcgc tttcataatc
     2821 ataccattcc acctgaagac caccgcccgc gaaagctgcc agggaaaacc tttctcccgc
     2881 gaaccaatcg agccccacct gggctatccc ccgcccaagc caggaagttt gatcattcca
     2941 ggggcctgct cccgcggctt gcggcgtgta cgtcgaatca tagctcgctc cgcccaccgt
     3001 cagccgaatc tgagggcgga cggtccaact gccatccccc aggaatagcc atccctccgc
     3061 atcaccatcg agtttcgccg tttcgatatg cgccatcgac gtgacttcat cataagcgag
     3121 cgtgctgccg cgcacgccaa ccccccagac cccggtgccg tagccaatcg cgccatccgc
     3181 tgccaagcgg tacgcgtcgc tcttcacccg tacggctccc aggttgccat cgaaaagcca
     3241 gcctgcccct gacccttcca tagccaagct cgcgggagca tgcgtcagca ccagcgtccc
     3301 ggggagcaaa cacagcgtcg agatttggag tccgaaaacg tccttgcgca tcttttccct
     3361 cggagttcac cattaaacca agagttcgcc aatggcaatg cattctcggg aaaacccggt
     3421 cgtgaaaatc gcggtcaact gtccacatcc cggcagaagg ccgccaccat tcctctgttg
     3481 atttccgtgg ctgattgcag caaactaagc acacgttcag tgtcctcgtt ttctaaaaaa
     3541 cttcaacgaa ttcaacaggt tggccctagt tcacccacgc cttcgccatc ctttgtcccc
     3601 gaggaggaaa cctcgccagt ccgttcctcg gattcgctcg aagcgcttcg ggaaaaaatc
     3661 gcaaaaatcg ttggaaggac cgtggctcct cctccggccc cctcgagtcg acctccccga
     3721 acacttcctt tcgttgagca tcccacaccg gaaggctcct tttttcaaca ccttatgtct
     3781 ctggaccctc agcaccacgt gggacgcttt cccatccgtc tcgcacaaag cgccaacagc
     3841 gccaccttgg gtctgctcgc tctcgatcct tgccttgcgt ctatcgatat acaacgagtt
     3901 ctgtacctgg atacggaaac gactgggttg gctggaggga cgggcacgct accttttctt
     3961 gttggaatgg gctcattttc ccaacagggg gctttcgtgg tcgagcagct ccttttgcgt
     4021 gcgccggggg aagagtatcc tttgttgcgt cggctgctcg acaatctcca gcgcgcttcc
     4081 gccatcgtga ccttcaacgg aaaaaccttt gatcttcctc tacttcgcac gcgtctggtg
     4141 atgaaccgtt tgccgccgat gcccgccttg cctcatctgg atctgcttca cgtggcgcgg
     4201 cgtatccaca agacgcgcat aggttcctgt catcttgggg cggtggagtc gcgggttctg
     4261 ggattcgagc gcgtgggtga tgtgccatcc gaccagatag cgggccggta ctttcacttt
     4321 cttcgcacgg gagacgagga agggttggtc gatgtggttt cccataatgt atgggatatt
     4381 gtgtcgatgg cagcgctggt ggggttgtac ggggagccct tgtccgtgtt gccgggagaa
     4441 gatctggctg gagtcgcggc cacggtggcc cgtgcccagg agcctgccca ggcagcagaa
     4501 atcgcggata ttgccgtgga aaaaggggga ggggtgccgg ccctgcgcct gcgtgcgacc
     4561 ctcgccaaag cccggggaga ccgacaggcg gccctacagg atttcgaaac gctggaaaga
     4621 gaagtcgatg accctggcgt ccgtctagaa ctggccaagc tttacgagca ccatctcaaa
     4681 gcgtatggac gcgccattcg tgtcgtcgag
//
LOCUS       DAOSOR010000473         4706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_18222_length_4706_cov_3.373038, whole genome shotgun sequence.
ACCESSION   DAOSOR010000473 DAOSOR010000000
VERSION     DAOSOR010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18222_length_4706_cov_3.373038"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1446
                     /locus_tag="PK710_19910"
     CDS             157..1446
                     /locus_tag="PK710_19910"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT12052.1"
                     /translation="MNESSAKITWLEQLHQVLLRVHLPVREMVLTQGRLRIDFGFRTD
                     RNLTVDIRPRDAASQCFSQTRRYSIGYDGQGPLDRSEQSGLERLVSVLQRIENRLPEK
                     WQGTAGVFLPSTPRSERFLRTFPFCTVERSQVGESWLTEVLVRTTSRCNQDCLFCTAP
                     IHETPLESDIYALLDELPEAFPHAMLSLTGGEPTLRPSFLREVVSASSREGIASVQVQ
                     TNAVLLGSKLDPQLIPVRPHLSFFVSLHDLDPERYDRCTHTRGQLAFALKGIRRLVDG
                     GHRVTINTVVSAINVEHLVDFAKQWPRALPVSDAIDWHLSALICPSGRPEAAEVLVPY
                     PTFVEQLQRAAAIAHQQGIVVQSVRFDPCVRTCLFFTPRMARTTIPSSASSRTRNGAP
                     RSTASVGQGRGMFTMHSQRVLLRGSEAVRAALRRSSP"
     gene            complement(1506..1676)
                     /locus_tag="PK710_19915"
     CDS             complement(1506..1676)
                     /locus_tag="PK710_19915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12053.1"
                     /translation="MTDPKPILNLVLPPETTRWRCQVCGIEHRGSSPPAVCPVCGVGP
                     DFWDPLSEPTDS"
     gene            1821..2222
                     /locus_tag="PK710_19920"
     CDS             1821..2222
                     /locus_tag="PK710_19920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12054.1"
                     /translation="MSSALRWLACFCVAGMIAAGACAINPQPEPPGVADDKGGMGGSG
                     GADVGAGVGDSGPGDGAIAPTVDATLFEDVLNTDDGNELGDSDDEGVDVVGGGDADEA
                     GDAEEEEAGDAADEGAADAGDGNVLMDVVDS"
     gene            2679..3890
                     /locus_tag="PK710_19925"
     CDS             2679..3890
                     /locus_tag="PK710_19925"
                     /inference="COORDINATES: protein motif:HMM:NF020049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOT12055.1"
                     /translation="MTRWESLRKQGRSTATLGFALLIALVSLAGCVGAVGEPESYGEG
                     TSTGEQAVSKVSSQTVLARAEGIAPHNRPYVFNGVQDCYGYVRQVWNAILYDGSMHSE
                     DFYPNSYNKSRWLGVSGGLPVADYPSSNWVYFSDPSVLLPGDVLSTHQGHAWGDTWHG
                     GLYAGVSNGQHRIWDNSGSGNGAYNRPFYWGFHYYYKPTHDMLAGKGSSSSGSSGSSG
                     STTKPSAGWVQEYGTFSPYFEINVRTAPNTSASIVATYGPGQSVNYDSYYNDGRYVWI
                     HYVSYSGYDRYMVCRENGTAWGSFSSGGAASSSSSSSSSGSSGSASSGGSKGWVTEYG
                     TFVPYFPINVRTGPSTSSSVVATYNAGESVNYDSYYNDGWYVWLHYVSYSGYDRYLVG
                     RENGTAWGDFY"
     gene            complement(4021..4509)
                     /locus_tag="PK710_19930"
     CDS             complement(4021..4509)
                     /locus_tag="PK710_19930"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HOT12056.1"
                     /translation="MAQPFFGLDDSSSFSETMEQVSDKDAGTAFLLSYLGGTFGLDRF
                     YMGQIGLGILKLLTLGGLGIWSLIDVILFGMGKIRDSQGRPLRRPPPVGNPTVDGPTL
                     LIVSMFLGSWGVDRFMLGQSGLAVAKMLTCGGFGVWSVVDLIMIGTGSMKDAQGNSLR
                     WP"
BASE COUNT         1057 a         1248 c         1390 g         1011 t
ORIGIN      
        1 ggcagtgtag atgcctcgat tgagcctgtc gatcggaatt acgaaagccg gagcgtagaa
       61 cgaaaagcta agggcaaagc cttcccaaag gcagaggggg agttgcgaag aacgacacga
      121 aatagccacg gtccatgcta ggcttgcagc attgtcgtga acgaatcatc cgcgaagatc
      181 acttggctcg aacagctcca tcaggtgctg ttgcgggttc atcttcccgt ccgagagatg
      241 gtgctcacgc aaggaagatt acgcatcgac tttggttttc gcacggatcg gaatctcacg
      301 gtggacattc gcccccgcga tgccgcatcc cagtgttttt cacagacccg tcgctattcg
      361 attggctacg atggccaagg gccgctcgat cgaagcgagc aaagcggttt ggagcgcctg
      421 gtttccgttt tgcagcgcat cgaaaaccgt cttcccgaga aatggcaggg caccgcgggt
      481 gtctttctcc cttcgactcc gcggtccgaa cgtttccttc gaactttccc gttctgtacg
      541 gtagagcgct cccaggttgg ggaaagctgg cttacggaag tattggttcg taccacctcg
      601 cgatgcaacc aagactgcct tttttgcacg gccccaatcc acgaaacacc tttagaatcc
      661 gatatttacg ctttattgga cgaactaccg gaggcttttc ctcatgccat gctcagcttg
      721 acgggcggcg agccaacact tcgtccatcg tttctgcgag aggttgtctc ggccagttcc
      781 cgtgaaggaa ttgccagcgt gcaggtgcaa accaacgccg tgcttttggg ctccaagctc
      841 gatccgcaac tcatccccgt tcgacctcat ctatcgtttt tcgtatcgct gcatgatttg
      901 gatccggagc gttacgatcg ctgcacccat acgcggggtc agctcgcctt cgcgttgaaa
      961 ggaatacgca ggctggtgga tgggggtcat cgagtcacca tcaatacggt ggtgagcgca
     1021 atcaacgtcg agcatctggt ggatttcgca aaacaatggc cgcgcgcgtt gccggtaagc
     1081 gatgcgatcg actggcatct ttccgctttg atttgcccat ccggacgacc ggaagctgcc
     1141 gaggttctgg tgccttatcc aacgttcgtc gaacaactgc aacgagcggc agccatcgcc
     1201 caccagcagg gaatcgtggt tcagtccgtg cgcttcgacc catgcgtccg tacctgcttg
     1261 ttttttaccc cccgaatggc aagaacaacc attccatcgt ccgcaagttc tcgaacacga
     1321 aacggggctc ctcgatcaac agcgagcgtg ggtcaaggcc gaggcatgtt cacaatgcat
     1381 tcacaacgcg tattgcttag gggttccgag gccgtacgcg ctgcgcttcg gcgttcgagc
     1441 ccttaggccc ttgaagcccc ctgccgaatt ggcaacaccg acccaagatc gcgagtgatc
     1501 cttgtttacg agtctgttgg ctcgctcagg ggatcccaaa agtccgggcc tactccgcaa
     1561 acggggcata ccgcaggagg gctggaacct cgatgctcga tgccgcaaac ctgacaccgc
     1621 cagcgggtgg tttcgggggg aagaacgagg ttcaagatgg gtttgggatc ggtcatggcg
     1681 cgaaccgagg gcggcaagcc tagcgtcgaa atatctaaaa cgccagtaaa taccagtagt
     1741 tataacacat tcgaaagcag cggcgagtca ttttgtcatc gcaacctcgc gggattgcga
     1801 ttcgatgcta cactcgtggc atgtcttcgg cactgcgatg gttggcttgt ttttgtgtgg
     1861 cgggcatgat tgccgcggga gcgtgtgcga tcaatccgca gccagagccg ccgggagtcg
     1921 cggacgataa gggtggcatg ggagggtccg gcggggccga tgtgggcgcg ggagtcggtg
     1981 attccggccc aggggatggg gccattgcgc caacggtcga cgcgactctt ttcgaagacg
     2041 tgttgaacac tgatgatggc aatgagctag gggatagtga tgacgagggc gtggatgtcg
     2101 tcgggggggg agatgccgat gaggcaggcg acgcggaaga ggaggaggca ggcgacgcgg
     2161 cggatgaagg ggcggctgat gccggggatg ggaatgtgtt gatggatgtg gtggattcgt
     2221 agggcgaacc gtcgtagcgt ggaaaagacc agtgcggaaa aagggtatgg gaagggcgat
     2281 acccttgcgg attacggtgc ggtgaggcgc ggtattgttc ggggtttggc aggcggtcat
     2341 tcccttcttt catgtaggca aagagatcac aaaaccctat aaaagaagat gtggcgcgat
     2401 attgacaccg tgctgccatt gaacacggtg tatttctcct ctgggatccg gcagatcccc
     2461 attgtcggac tcggaacgtg gcgatagctc gataaaaagc ctggaagtca ccatggcatg
     2521 aagaatgctc cggtgtgtga ggaatgagaa ccccgaagac gagaccactt gcttatcgcc
     2581 ttggagaagg ttgggtaggg ttgagagcga gggcttattt tcccgtgctc ggcagtggac
     2641 ttccacatag agttttgggc caacagatgg aggcagcgat gacaagatgg gagagtttgc
     2701 gaaagcaggg gcgtagcacc gccactctgg gattcgcctt gttgatcgcc cttgtttcgc
     2761 tagcgggatg cgtgggcgcg gttggggaac ccgagtcgta cggagaaggg acaagcactg
     2821 gggaacaagc cgtttccaag gtatcgtcgc agacggtcct ggcgcgcgcg gaagggattg
     2881 ccccccacaa tcgcccgtat gtgttcaacg gagtgcaaga ctgctacggt tatgtgcgtc
     2941 aggtatggaa tgccatttta tacgatggct cgatgcatag cgaggatttc tatccgaaca
     3001 gctacaacaa gtcacgctgg ctcggggtgt ccggggggct tccggtagct gattatccca
     3061 gcagcaactg ggtctatttc tcggacccca gcgttttgtt gcctggcgat gttctttcca
     3121 cacaccaggg gcacgcctgg ggcgatacct ggcacggcgg gctttatgcc ggagtcagca
     3181 atgggcaaca tcgcatctgg gacaacagtg gaagtggaaa tggggcgtat aatcgtcctt
     3241 tttactgggg attccactac tattacaaac ccacccacga catgctcgct gggaaaggtt
     3301 ccagttccag cggtagcagc ggttcgagcg ggtccaccac caagccctcg gcgggatggg
     3361 tccaagaata cggcacgttc agcccgtatt tcgaaatcaa cgtacgcacc gctccgaata
     3421 cgtccgcgag tatcgtagcc acctacgggc ccggccaatc cgtaaattat gattcttact
     3481 acaatgatgg ccggtatgtc tggattcact acgtatctta ttctggctac gatcgatata
     3541 tggtatgccg cgaaaacgga acagcctggg gaagcttttc gtcgggggga gccgcgagca
     3601 gctccagttc ctcgagcagt tccggctctt caggcagcgc cagtagcgga gggtcgaaag
     3661 gctgggtgac ggaatatggc acgttcgttc cgtacttccc catcaatgtg cgcaccggcc
     3721 cgagcacgtc gtcgagcgtg gtagccacct acaacgctgg cgaatcagtc aattatgatt
     3781 cctactacaa cgatggctgg tatgtgtggc tgcattatgt ctcgtatagc ggttacgatc
     3841 gctacctggt aggcagggag aacggaacgg cgtgggggga cttttactaa gccagaaatc
     3901 acttgaagct ccctgctagc aggtaaccgt ttctcgattc ccaccctact ttcacccccc
     3961 tgccctttct tacccgctat ccgaccggaa accccggaca tcccaacggc aaaagagggg
     4021 tcacggccag cgaagggaat ttccctgcgc atctttcatg gatccggtcc caatcatgat
     4081 caggtccacg acggaccaaa cgccaaaccc accacacgtc aacattttgg caaccgccag
     4141 tcccgactgc cccaacatga aacgatccac accccaacta ccgagaaaca tagatactat
     4201 caacagagtc gggccgtcga cagtgggatt tccgacagga gggggacgtc gcagcgggcg
     4261 tccttgcgaa tcgcgaattt ttcccatacc gaataggatg acatcgatga gggaccaaat
     4321 tcccaatcct cccaaggtca ggagtttgag aatccccaag ccgatttggc ccatatagaa
     4381 gcggtccagt ccaaacgtgc cgcccaaata ggaaagaaga aaagcggtgc cggcatcctt
     4441 gtcggaaact tgttccatcg tttcggaaaa cgacgatgag tcatccaagc caaaaaaggg
     4501 ttgtgccatt gaacgatttc tccttgaaat tcagcaattt gaatgattcc cgggaccatc
     4561 gcagagcaag tagccgccat gagtctaccg tgatggtgcg aaatagaaag agtttacggc
     4621 gctttcgagg gcgaattcca aacgctgtga gcttgtcttt cgctgccatc gaatgctata
     4681 ccaacttcgg ccatgagaaa gagctt
//
LOCUS       DAOSOR010000474         4701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_18243_length_4701_cov_4.753551, whole genome shotgun sequence.
ACCESSION   DAOSOR010000474 DAOSOR010000000
VERSION     DAOSOR010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18243_length_4701_cov_4.753551"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="PK710_19935"
     CDS             complement(<1..912)
                     /locus_tag="PK710_19935"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOT12057.1"
                     /translation="MKDRIALIADDEPAHRRMLGAALESIGMQCRYAVDGEEAVREVA
                     VFTPHLLVLDLRMPRLDGLGALKRIHELLPTLPVVVVTAHGDIRAAVEAMKLGARDFL
                     EKPIDIEELRAVALDLLDRPGPAARSPAADADQDEPRSLVGISSSMRQARETIRLAAR
                     SAATVLVRGESGTGKEVVARAIHELSDRQAGPFIAMNCAAVAEGVLESELFGHERGSF
                     TGAEARRRGRFELADGGTLFLDEVAEMQPHLQAKLLRVLQERCFERVGGVTPIRTDIR
                     VVAATNRIPTEEIASGRMREDLYYRLAV"
     gene            complement(902..2329)
                     /locus_tag="PK710_19940"
     CDS             complement(902..2329)
                     /locus_tag="PK710_19940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT12058.1"
                     /translation="MHSSQPPKTTGWISGAVALLLLTIAFLVSWQTWSGLDHVQRMMT
                     DRSNTLHRMVATELRNVARYGAARRERLDAVLQEIANSKDIEGVLLERDDGAVRFLHG
                     TVPQVAERQGQFTLGKTLVVTGPVSIETLGCLELPNEDKTAASACPHEHDAPSDDPSN
                     ELPSCCAADSSSGHVGCSGGPSTGLDGTYRVTLAIDATPYLRIRRSVIYQATAGGLVL
                     LALALALGRHQRQMRRHAQVTQALAIADERARYLERLSLVAGGLAHEIKNPIGALRGF
                     AQLIGEKMEAGSAEAEYVSLMVSELDSITRRVDGLRQFARPAPIQLQSAYPTEIVQRI
                     AALLAPDIASRKLSMHLDLPPSPGPKGMIDADRLRDLIVNLMTNAIEASPEGGTVYVR
                     FMLDQDTQTFLFEVQDEGPGIPVSEREGVLRPFHSTKPDGLGLGLALAQRAVEDHRGT
                     LEIDSSSHGGTAVRARWPRSATREG"
     gene            complement(2360..2986)
                     /locus_tag="PK710_19945"
     CDS             complement(2360..2986)
                     /locus_tag="PK710_19945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12059.1"
                     /translation="MNHKMYYAIVISLLSALAALVIGCAEHETPTTGETVAPVAAEVK
                     NKQCPGFVDNDGDGRCDKAATGECGCASGACDCGKAGGCDCANGGNCECSKDGGSCNC
                     AKAGGCDCANGGNCECSKDGGSCQCAKAGGCGCANGGSCDCSKDGGSCNCAKAGGCDC
                     KAGGHGCAKMGHGSCPKAKGHHHSEAAMPIKAGECGCSKKAEKPASHI"
     gene            complement(3199..>4701)
                     /locus_tag="PK710_19950"
     CDS             complement(3199..>4701)
                     /locus_tag="PK710_19950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12060.1"
                     /translation="VTLTAGKQNATFQVSVTPPTNGGAPISFQVAVSDKPIGSIGVSM
                     AYLGELKLENLVPSLYQGVTCDKLKADDLPTPIMTTSPALATVNDTTAFVALNVAKDY
                     AVTVTAKVGPNIRAFGCVDNIEVVQSDETKIKVPLADLEWPGPVLGTYDLTNLLDFGG
                     TLPESVELAVTILDELTDDHAPNGGCDMANEWYGEDPGAFITDFLMRQTCHWECKPGE
                     DFDNCSQINHGWGDIGAVCQQNMTSWDGGKPRFFGGCGAWETAAPFVQNQINDLMKQY
                     IPEGVLAFANMAGDLARAINKAKIYSQLNVQQGSDTAQPMTHRLVEMEVLLHDLKGKE
                     HIYKFNLADVGLKSLQTNANLSVDGIKVTIPEHEFKLSYGKLIQYIYLHGLLPLFGYT
                     STADMIQHWVNCTAVGQWIFDKTGFASAQDWAQYCTMGITLAGDTLDSQISGFIKSEG
                     TLKLQGTCDATDIDINNIAQTLSNGKWLGQWGEDAGSVGNVSGTFTGKQH"
BASE COUNT          945 a         1366 c         1322 g         1068 t
ORIGIN      
        1 aacagccaat cggtaataca aatcctctcg catacgtccc gaggcgattt cttcggtagg
       61 aattcggttg gtggcggcca ccacgcgaat atcggtgcga attggtgtca caccaccgac
      121 tcgctcgaag cagcgctctt gcagcacacg caagagcttg gcctggaggt gcggctgcat
      181 ttccgcgact tcatccaaaa agagtgtgcc gccgtccgcg agttcgaacc gtccacgacg
      241 tcttgcttcc gcgccagtga atgaacctcg ttcgtgtccg aacagttcac tttcgagcac
      301 accctcggca actgccgcgc aattcatcgc gataaaaggg cctgcttgtc gatcggacaa
      361 ctcatggatg gcccgtgcga cgacttcttt ccccgtgccg ctttctcctc gcaccaatac
      421 ggttgccgca gagcgtgcgg cgagccgaat cgtttcgcga gcctggcgca tcgaggaact
      481 gatgccgaca agcgatcgtg gttcgtcttg gtcggcatcc gccgcgggag agcgtgcggc
      541 cggtccgggt cggtccagca aatcgagcgc cacagcgcga agctcttcga tatcgatcgg
      601 tttttccaaa aaatctcgag cccccaattt catggcctcg accgccgctc gaatatcgcc
      661 gtgcgctgtc accacaacca cgggtaacgt gggcaacaat tcatgaattc tttttagcgc
      721 cccgagacca tccaatcgcg gcatgcgcaa gtccagcacc agtaaatgtg gtgtaaatac
      781 agctacttcc cgaacggctt cctccccatc gaccgcgtac cggcattgca ttccaatgct
      841 ttcgagcgcg gctccgagca ttcgtcggtg agccggttcg tcatccgcaa tgagcgctat
      901 cctatccttc acgggtcgca ctcctcggcc atcgggctcg cacagccgtt cctccatggg
      961 atgaggaatc gatttcaaga gttccacgat ggtcttccac ggctcgttgt gccaacgcca
     1021 atcccaaccc gagcccatcg ggtttggtgg agtggaacgg acgaagaacg ccttcccgtt
     1081 ccgacaccgg gattcctgga ccctcgtctt gcacttcgaa caagaaagtc tgcgtgtctt
     1141 gatcgagcat gaaacgaacg tacaccgttc cgccctcggg cgaagcctcg atggcattgg
     1201 tcatgaggtt cacgatcaag tccctcaatc ggtccgcatc gatcatgccc ttgggacccg
     1261 gggatggtgg caaatcgagg tgcatcgaaa gcttacgaga agcgatgtct ggcgcaagca
     1321 gggccgcgat acgttgtacg atctccgtgg gatacgccga ttgcaactga atgggtgccg
     1381 gacgggcgaa ttgtcgaagt ccatcgacgc gacgcgtgat gctgtcgagt tcgctcacca
     1441 tcaaacttac gtattccgct tcggcggaac cggcttccat tttttctccg atgagttgtg
     1501 caaatcctcg aagcgctcca atggggtttt tgatttcatg ggcgagcccc ccagcgacca
     1561 gactcaatcg ttcgaggtac cgggctcgct cgtcggcgat cgccaacgct tgtgtgacct
     1621 gcgcatgtcg gcgcatttgt cgttggtgtc gaccgagcgc caatgccaag gcaagcaaca
     1681 cgagtccgcc agcggttgct tgataaatca cggaacgacg gattcgtaaa tacggtgtgg
     1741 cgtcgatggc gagagtcacg cgataggtgc catcgagacc cgtcgaaggt ccccccgaac
     1801 aacccacgtg cccagaggat gaatccgcgg cacagcagga aggcaactcg ttggaagggt
     1861 catcggacgg cgcgtcatgc tcatgggggc atgctgaggc tgcggtctta tcctcgttgg
     1921 gaagttccag gcatccaagc gtttcgatgg ataccgggcc cgtgacaacc agcgtttttc
     1981 ctagtgtgaa ttgaccttga cgttccgcca cttgaggaac cgttccgtga aggaaacgga
     2041 cggcaccatc gtcccgctct agcaaaaccc cttcaatatc tttgctgttt gcgatttcct
     2101 gcagcacggc gtccaatcgc tctcttcgcg cggcaccata acgagcgacg ttgcgaagtt
     2161 ccgtggccac catgcggtgc aacgtgttgc ttcggtcggt catcatgcgc tgcacgtggt
     2221 caagacctga ccacgtttgc cagcttacga gaaaagcgat ggtgagcagg agcagggcga
     2281 cggcgccgga aatccagccc gtggttttgg ggggctggga agaatgcatt acgtgatcct
     2341 ggtgttggag cgagggcgat cagatgtgag aggcgggttt ttcggctttc ttgctgcagc
     2401 cacattcccc ggccttgata ggcatcgcgg cttcggagtg atgatgtcct ttggctttgg
     2461 ggcaactgcc gtgtcccatc ttggcgcagc catgtccacc agctttgcaa tcgcatccgc
     2521 cagccttggc acaattgcaa gagccaccat ccttggagca atcacagctt ccaccgttgg
     2581 cgcatccgca tccaccagcc ttggcgcatt ggcaggagcc accatccttg gaacattcac
     2641 agtttccgcc atttgcgcaa tcgcagccgc cagccttcgc gcaattgcag gagccgccgt
     2701 ccttggaaca ttcacagttt ccgccgtttg cgcaatcgca gccgccagcc ttcccgcaat
     2761 cacaggcgcc gctggcgcaa ccacattctc ccgtggctgc cttatcgcat cgtccatcgc
     2821 catcattgtc cacgaatccg gggcattgtt tgttttttac ctccgccgca acgggagcaa
     2881 ctgtttcacc tgtggttggg gtttcgtgtt ccgcgcagcc gatgacaagc gccgcgagcg
     2941 cagacaacaa cgaaatgaca atggcgtaat acatcttgtg attcatcagg gtgtctcctt
     3001 gtgtgttgat tggatgacgt cccgtatgcc ggattcatgc caagctcgga tctggggacg
     3061 atccgaggat tgcgcaaggg gatgaagtgc agtgcgcagg tcttgcgcag tgagcgcgga
     3121 agacgttcat acaactgcgc cgaacatgcg cagcgcaagt agcgcatgca gacaggatgg
     3181 tagggagatg ggagggaatc agtgttgttt gccggtgaac gtaccgctta cattgccgac
     3241 gctaccagcg tcctcgcccc actgaccgag ccatttgcca ttgctcaatg tttgggcgat
     3301 gttgttgata tcgatatccg tggcatcgca ggtgccttgt agtttgaggg taccctcgga
     3361 tttgatgaag cccgagatct ggctgtccaa cgtatctccc gcgagggtga tgcccatcgt
     3421 gcagtattgt gcccagtcct gcgccgaagc gaatccggtt ttgtcgaaga tccactgtcc
     3481 gacggccgtg cagttgaccc agtgttggat catgtctgcc gtgctggtgt agccgaacag
     3541 aggcagcagt ccgtggaggt agatgtactg gatgagcttg ccataggaca gtttgaactc
     3601 atgctcgggg atcgtaactt tgatgccatc gacagagaga ttggcgttgg tctgcagcga
     3661 cttgagtccc acgtccgcca aattgaattt gtaaatgtgc tccttgcctt tcaggtcgtg
     3721 caagagcact tccatttcca ccagtcggtg ggtcatgggt tgcgcggtat cggatccttg
     3781 ttggacgttg agttgcgaat aaattttggc tttgttgatg gcgcgcgcga ggtcgcctgc
     3841 catgttggca aaagccaata ccccttcggg gatgtattgt ttcatcaagt cgttgatttg
     3901 gttttggaca aatggagcgg ccgtttccca agcaccgcat ccaccgaaga agcgaggctt
     3961 cccgccgtcc caactcgtca tattctgttg gcaaaccgct ccaatatcgc cccagccatg
     4021 attgatttgg ctgcaattat cgaagtcctc ccccggtttg cactcccaat gacaggtctg
     4081 gcgcatcaag aagtcggtaa tgaacgctcc tgggtcctca ccgtaccatt cgttcgccat
     4141 atcgcacccg ccgttgggcg cgtggtcatc cgtcagttcg tcgagaatgg tcacggccaa
     4201 ctccacggac tcagggaggg tgcctccaaa atcgagaaga ttcgtgaggt cgtacgtgcc
     4261 tagcaccggt cccggccact cgagatccgc aagaggaacc ttgatcttcg tttcatcgga
     4321 ttgcaccact tcgatgttgt ccacacaacc gaaagcccga atgtttggtc ccaccttggc
     4381 cgtgaccgtg accgcgtaat ccttggccac attcaacgcc acgaacgctg tcgtatcgtt
     4441 gaccgtcgca agcgccggcg acgtcgtcat gatcggggtg ggtaagtcat ccgcctttag
     4501 cttgtcacaa gtaacgcctt gataaagcga gggcacgagg ttctcgagct tcaactcgcc
     4561 aaggtacgcc atcgacacac ctatgcttcc gataggctta tccgataccg ctacctggaa
     4621 agaaatcggt gcgcctccgt tggtgggcgg tgtgacgctg acttggaacg tggcgttctg
     4681 cttgcctgcg gtcagcgtta c
//
LOCUS       DAOSOR010000475         4689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_18306_length_4689_cov_4.004100, whole genome shotgun sequence.
ACCESSION   DAOSOR010000475 DAOSOR010000000
VERSION     DAOSOR010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18306_length_4689_cov_4.004100"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            348..1178
                     /gene="rfbF"
                     /locus_tag="PK710_19955"
     CDS             348..1178
                     /gene="rfbF"
                     /locus_tag="PK710_19955"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HOT12061.1"
                     /translation="MGRRSSEGFQGTGTSLKAIILCGGMGTRLREQTEVKPKPMIEVG
                     GYPLLLHIMRIYAHHGIRDFVLCLGYKGAVIKQYFLQIEALLGDFTLEFGAERKIHYH
                     GMGARDLRDWRVTFVDTGESTQTGERILRASKYLNDETFAVTYGDGVADIDLAAALAF
                     HRSHGKLATVTGVRPPSRFGEIEAKDGEVLSFNEKPQVAQGLINGGFFFFEPAFLKNI
                     KQVPHAALEREALEKCVKDRQLQVYEHRGFWHCMDTYRDWQNLEEMWNSGAPPWRMWT
                     "
     gene            1175..2296
                     /gene="rfbG"
                     /locus_tag="PK710_19960"
     CDS             1175..2296
                     /gene="rfbG"
                     /locus_tag="PK710_19960"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR02622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HOT12062.1"
                     /translation="MTPLDVIRGRSVLVTGHTGFKGSWLCLALHRLGAIVHGLSLPPP
                     TQPNHYELARIAALLRTEMLGDVRDAATVRQALQRANPDLVLHLAAQPIVRQGYEFPE
                     ETFATNILGTLHILEAVRQRRTPCVLLIVTSDKVYEPGKDVPSHREDDPLGGHDPYAA
                     SKAAAELVVQAYRRSFFPPEKLAFHGVKLASARAGNVIGGGDWAQDRIVPDSIRALTQ
                     NRPIIVRNPRAIRPWQHITDVVHAYLTLVATMCASDEPERFSAWNFGPPDGKDVRVAD
                     LVAEICAVWGQGSWMTEALADVPFEAPVLRLDSHKATSRLGVRTYFEPRQAIELTVRW
                     YRAWHEGMDDMQGVSIRDLEAHDRARLHGGPTSSEVQGE"
     gene            2289..3671
                     /gene="rfbH"
                     /locus_tag="PK710_19965"
     CDS             2289..3671
                     /gene="rfbH"
                     /locus_tag="PK710_19965"
                     /inference="COORDINATES: protein motif:HMM:NF011936.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HOT12063.1"
                     /translation="MSDSTKEWQKDAGASAMDEIPEDDAALEHKILRLTGELIRRRLG
                     KRRPFKPGITSIPYAGRVYDEREVQAAVQASLDFWLTLGPEGQAFERELAEYLGVKRA
                     LMVNSGSSANLIALMSLTSEQLDRPLRKGDEVITVACGFPTTVNPIVQAGCIPVFVDV
                     DMTTLGVDVTQLEAARSERTRAVLLAHTLGNPFDLDAVLSFCRAYDLVLIEDNCDALG
                     SRYRGQLTGTFGNFGTSSFYPAHHITAGEGGAVYTHDLHLAKIAESFRDWGRDCWCAC
                     GRDNTCGKRFAWEWDDLPAGYDHKYVYRHLGYNLKPTDIQAAIGRVQLRKLGEFGAAR
                     RRNWARLRQLLSPLEGRVNFVQATPGADPSWFGLAMVLQQPDHEVLHRICAILDAKRV
                     GHRRIFAGNLLRHPAYRHVEHRKVGLLPVSDHVLAGGFFVGVYPGLDDQMMETQAKTV
                     IEAVEQAQRR"
     gene            3983..>4689
                     /locus_tag="PK710_19970"
     CDS             3983..>4689
                     /locus_tag="PK710_19970"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT12064.1"
                     /translation="MTETQLKRSVLWVTIFASFLTPFMGSAVNLALPAISEDFHLNAV
                     SLNWVISSYMLTTSIFLLPAGRAGDILGRRKVFSIGVMIFTLSMVALTLSTHFAWLIG
                     ARIVQGIGGAMMFGTNMAILTSVFPPGERGKAMGINVTAVYTGLASGPFLGGVLTRYL
                     GWRSIFAVLVPLGIVSWILLQTKVKQEWAEAKGERFDWLGAILYGLSIASLMYGVSVI
                     FTIQGKVLIAVGAVLVP"
BASE COUNT          927 a         1204 c         1474 g         1084 t
ORIGIN      
        1 tcggcttcct ggcgatgccc cggccttcca cgcgatcacg gcgtggcata gtttggagca
       61 cgttcataac ccattagaat tgttgactaa aattagggat cggctggagc gcgatggcgt
      121 tcttctcctg gccattccca acaaccaaag cttgcaatcc agggtagggg gacgccgttg
      181 gttgcacctg gatgtgccca gacatcttgt tcacttcgat gaacgctcct tgacgatgat
      241 gctgcgtcgc gcggggcttc gcgtgaagaa acgtttccct ttccgaactc gaggcgcctc
      301 tttgtcccgg accctcgttg cgccatcgtt ctgggacaac tacgatcgtg gggcgacgat
      361 cgtcggaagg ttttcaaggc acaggcactt cattgaaagc gatcatttta tgtggcggca
      421 tgggaacgcg tttgcgggag caaacggaag tcaagcccaa gcccatgatt gaagtcggcg
      481 gttacccttt gctgcttcat atcatgcgga tttacgcgca ccatggcatc cgcgatttcg
      541 tgctctgcct cggctacaaa ggtgccgtca tcaagcagta tttccttcaa atcgaagcgc
      601 ttctcggtga cttcaccctc gaattcggag ccgagcggaa gattcattat catgggatgg
      661 gcgcacggga ccttcgagat tggcgcgtga ccttcgtgga taccggcgaa tcgacccaga
      721 cgggcgaacg gattctgcgt gcttcgaagt atctgaacga cgagacgttt gcggtgacgt
      781 atggcgatgg cgtggccgat atcgatttgg ccgccgcgct ggcgtttcat cgttcccacg
      841 gcaagctggc taccgtaacg ggcgtgcggc ctccgagccg cttcggggag atagaggcga
      901 aagatgggga agtgctttcc ttcaacgaaa aaccgcaggt ggcccagggg ctcatcaacg
      961 gtggtttttt ctttttcgaa ccggcctttt tgaagaacat caaacaagtg cctcatgcgg
     1021 ccttggagcg ggaagccctc gaaaaatgcg tgaaagatcg ccagctacag gtctacgaac
     1081 accgcggttt ctggcattgc atggacacct accgtgattg gcaaaacctc gaggaaatgt
     1141 ggaattccgg cgcgcccccg tggagaatgt ggacgtgacg ccccttgatg tcatccgggg
     1201 gcgatccgtt ctggtcaccg gccatacggg cttcaaaggc tcatggcttt gtctcgcgct
     1261 ccatcgtctt ggcgcaatcg ttcacggtct gtcgctgccc cctccgacgc aaccaaacca
     1321 ttatgaattg gcgcgcatcg cagcgctcct tcgcacggaa atgttggggg atgttcgcga
     1381 tgccgccacg gttcgacagg ccctgcaacg tgccaacccg gacctcgtcc tgcatctggc
     1441 cgctcagccc atcgtgcggc aaggctatga atttccagaa gaaacctttg ccacgaacat
     1501 cctcggcacc ctgcatatcc ttgaagccgt gcggcagcga cgcacgcctt gtgttctgct
     1561 catcgtcacc tccgacaagg tatacgaacc ggggaaggac gttccctcgc atcgcgaaga
     1621 tgaccctctt ggtggtcacg atccctatgc agcgagcaaa gccgccgcgg aactcgtcgt
     1681 ccaggcctat cgtcgatcct tttttccccc cgaaaaactc gcctttcacg gtgtcaaact
     1741 cgccagcgct cgcgcgggaa acgtgatcgg gggaggcgat tgggcgcaag atcgaatcgt
     1801 gcccgatagc attcgagcgc tcacgcaaaa tcggcccatc atcgttcgca atcctcgagc
     1861 cattcgcccc tggcagcata ttacggacgt cgtgcatgcg tatctcacgc ttgtggcgac
     1921 gatgtgtgcc agcgatgagc ccgagcgttt ttccgcgtgg aatttcggtc ctcccgacgg
     1981 caaagacgtt cgggtggcgg atttggtagc ggagatatgt gccgtgtggg ggcaaggcag
     2041 ttggatgacg gaggctcttg cggacgtccc cttcgaagcg ccggtgttac gcttggattc
     2101 gcataaggca acgtccaggc tcggggttcg aacatacttc gaacctcggc aagccatcga
     2161 attgaccgtc cgatggtatc gcgcgtggca cgaaggcatg gatgatatgc aaggggtatc
     2221 gattcgcgat ctcgaagccc acgatcgcgc gcgtcttcac ggcgggccga cgtcttcgga
     2281 ggtgcaaggt gagtgattcc accaaggaat ggcaaaagga tgccggggcg agcgcgatgg
     2341 acgagattcc ggaagatgat gctgcattgg agcataaaat cctgaggttg acgggggaat
     2401 tgattcgacg acgccttggg aaacgtcggc ccttcaagcc tggcatcacc tcgattcctt
     2461 atgcgggccg ggtatatgac gagcgtgagg tgcaggcggc ggtccaggcg tcgctcgatt
     2521 tttggcttac cctcggcccg gaggggcaag cttttgaacg ggaattggcg gaatatcttg
     2581 gagtcaagcg cgcgttgatg gtcaattccg gttcgagcgc caatctcatc gcgctgatgt
     2641 ctctgaccag cgagcagttg gatcgtcctc tgcgcaaagg cgatgaagtc atcacggtgg
     2701 cctgtggatt tcccaccacg gtcaatccca tcgtgcaggc ggggtgcatc ccggtattcg
     2761 tcgatgtgga tatgacgacg cttggggtgg atgtgacgca gctcgaggcg gcgcgcagtg
     2821 agcggacacg cgcggttcta ttggcccata cccttggaaa tcctttcgat ctcgatgcgg
     2881 tgctctcgtt ttgtcgggcc tatgatctgg ttctcatcga ggataattgc gacgcgctgg
     2941 ggagccggta tcgaggccaa ctaaccggaa cctttgggaa ttttggtaca tcgagttttt
     3001 atcccgcgca tcatatcacg gcgggggagg ggggggctgt ctatacccac gaccttcatc
     3061 tggcgaaaat tgccgagagt tttcgggatt ggggacgcga ttgctggtgc gcttgcggtc
     3121 gcgacaatac ctgcggcaaa cgctttgcgt gggaatggga tgatttgccc gcgggctacg
     3181 accataagta cgtataccgg catcttggct acaacctgaa gccgacggac attcaggcgg
     3241 ctattgggag ggttcaactt cgcaagctcg gtgaatttgg cgcggcacgt cgccgtaatt
     3301 gggctcgact gcggcagttg ctttcaccgc tcgaaggtag ggtgaatttc gtgcaggcca
     3361 cgccgggggc cgatccgagc tggttcggat tggcgatggt gttgcaacag ccggatcatg
     3421 aggttttgca caggatttgt gcgatactcg atgcgaaacg agtggggcat cgtcggattt
     3481 tcgcgggaaa tttgctgcgg catccggctt atcgccatgt ggagcatcgc aaggtggggc
     3541 ttttgccggt ttccgatcac gttctcgcgg ggggattttt cgtgggggtg tatccggggc
     3601 tcgacgatca aatgatggaa acgcaggcta agacggtaat cgaggcggtg gagcaggccc
     3661 agcggcgctg aaggtggtaa tataagtggt taggggttgg gttgtaggaa gcggagccgg
     3721 tgccaggcgc aacccggggg atgcgggaat ggaaaacgaa tcataggatc ggtatcaacg
     3781 aaaattccgg tattagatga ccatacggct cagcgtcgac gaaaaaactg gggctttggg
     3841 agggatatta tgaattttct acgatagagg cactggtcgt ggggttcgtc gtgcagaaag
     3901 atggttcgtg tctgcatttt tctccgtgcg gcttttgctg gggaggaata gggtttgcgg
     3961 gcaaggaata ggacagcagc ggatgacaga aacccaactc aagcgctcgg tgttatgggt
     4021 cacgattttt gcgtcgtttc tgactccttt catggggtct gcggtcaacc tggcgcttcc
     4081 ggccatcagt gaagatttcc acctcaacgc ggtgagtctc aattgggtga tcagcagcta
     4141 catgctgacg acctcgattt ttcttcttcc cgcgggtcgg gccggggata ttctcgggcg
     4201 tagaaaagtg ttttccatcg gggtgatgat tttcacgcta tcgatggtgg cgctcacctt
     4261 atcgacccat ttcgcatggc tcattggagc acgaattgtg cagggcatcg gaggggcgat
     4321 gatgttcggg accaacatgg caatcctgac gtccgtattt cctccggggg agcggggcaa
     4381 ggccatggga atcaacgtga cggcggtgta tacgggactt gcctcgggac cgtttctggg
     4441 gggcgtcctt actcggtatt tggggtggag aagcatattt gcggtcctgg ttccgttggg
     4501 gatcgtgagc tggattctgc tacagaccaa agtcaaacaa gaatgggcgg aggcaaaagg
     4561 ggagcgtttc gattggctgg gagctatctt atatggtctt tcgattgctt cgttgatgta
     4621 cggggtttcc gtgattttta cgatacaggg caaggtcctc atcgcggtgg gcgcggtgct
     4681 ggtgccgtt
//
LOCUS       DAOSOR010000476         4686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_18321_length_4686_cov_3.688404, whole genome shotgun sequence.
ACCESSION   DAOSOR010000476 DAOSOR010000000
VERSION     DAOSOR010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18321_length_4686_cov_3.688404"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PK710_19975"
     CDS             complement(<1..565)
                     /locus_tag="PK710_19975"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOT12065.1"
                     /translation="MWTLDAPASHEAIIKKSRFVACATRVDSPQEAAEYLESLHDPHA
                     THHCWAYRIGLLYRFSDDGEPGGTAGRPILAAIEGQQFDHIMVVVVRFFGGIKLGAGG
                     LVRAYGGVTAQCLRLATRVEIIPRVTLRVGAPFHALGALYLLFERFSATKIDEQFDAD
                     GAVFRIELEQKALDDFRGAVSDATRGAG"
     gene            843..1466
                     /locus_tag="PK710_19980"
     CDS             843..1466
                     /locus_tag="PK710_19980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HOT12066.1"
                     /translation="MIDLYTYNTPNGRKVSIALEELGLPYRVHIVDILAGQQFSPEFL
                     AISPNNKIPAIVDSEGPEGKTVHLFESGAILLYLAEKTGKLIPADPMGRLSTWQWLFW
                     QVGGVGPMFGQAGHFVHYAKEKIPYAIERYTSEVNRLLKVLEQQLAKQGPYLVGETYS
                     IADIAVAPWVDGLSFYGLAELLEPHEHIRRWLETFKARPAVQKGWNV"
     gene            complement(2139..2606)
                     /locus_tag="PK710_19985"
     CDS             complement(2139..2606)
                     /locus_tag="PK710_19985"
                     /inference="COORDINATES: protein motif:HMM:NF007807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HOT12067.1"
                     /translation="MHSEITMPFDIRPFRNDDTEALVEIWRRSVRATHDFLKEEDFRD
                     IEQKLATVYFPNVEIYVACSGDDRPVGFIGMNGRHIEMLFLDPLVMGLGMGRKLLEHV
                     MAGDGPLTVDVNAQNPKAVGFYVHCGFVPTGRSETDSEGRAYPLLHLAMVGDG"
     gene            complement(2631..3290)
                     /locus_tag="PK710_19990"
     CDS             complement(2631..3290)
                     /locus_tag="PK710_19990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12068.1"
                     /translation="MPLSDERKRVYPCSIAVFSYDLTTLSKPWASHTTPRKPPLWVAL
                     ALVSAVVGCAGRPAEPVRPNAAFVVNIEAVAAGDGAPGIRFDGPTGEIGVLRLERDDD
                     VAPGAMPLRVENMGQIGKAALLVMDTNPSVAGPMSHCQAGEEQFLRVVNLKGQRPIET
                     FRLKTASCRANTELAAAGIKGSDRDLTLTVEWLLGPKKGNPQRRIIRFDTHGNPISDS
                     G"
     gene            complement(3325..3546)
                     /locus_tag="PK710_19995"
     CDS             complement(3325..3546)
                     /locus_tag="PK710_19995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12069.1"
                     /translation="MSKRSGTAFTDMRLAVRTSAKNDVLFEKGAIFDGFRAQRHCPAA
                     LYLIEVDALRRPLEGIAPGELPRWTEALS"
     gene            complement(3620..4189)
                     /locus_tag="PK710_20000"
     CDS             complement(3620..4189)
                     /locus_tag="PK710_20000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12070.1"
                     /translation="MKSSTVVGDSAFKRYLPDPKAPSPAYRSLQNHLKTRCSGVEWSI
                     TPLLFSVADWQFFSVDCLEARDLPRELYAVHATRGVVEKNDMGVLGEIYHTLDIVGVG
                     TTKARDLAEIAACIQRSRGTVLDEVLLKSISRREGLEAPRLSKESGKATLVYFEQVGG
                     RRLAYRRCEVSISQDVQVDCAEFAPGGRQ"
BASE COUNT         1041 a         1301 c         1323 g         1021 t
ORIGIN      
        1 ttccggcccc ccgggtggca tcgctcacgg ctccacggaa atcatcgaga gctttttgtt
       61 ccagctcgat acgaaaaaca gccccatccg catcgaattg ttcgtcgatt ttggtggcgg
      121 aaaagcgctc gaagagcagg tatagcgcgc cgagggcgtg gaaaggagcg cccacgcgaa
      181 gcgtcacacg aggaatgatt tcgacacggg tagcgagacg caggcattgg gcagtcacgc
      241 caccataggc tcgcacgagg cctccggctc ccagcttgat accgccaaaa aatcgcacga
      301 caaccaccat gatatgatcg aattgctgtc cttcgatggc ggcgagaatg gggcgtccgg
      361 cagtgccgcc aggctcaccg tcgtcggaaa agcggtagag aaggccgatg cgataagccc
      421 agcagtggtg ggtggcatgg ggatcgtgca gggattcgag gtattcagcg gcttcttggg
      481 gggagtccac gcgcgtggcg caggccacga agcggctttt tttgatgatg gcttcgtggg
      541 atgcgggggc gtccaaggtc cacatgggaa gcagtctact gcaagtgatg gacaggttcg
      601 agtgttggag aggttttcgt cgccctggga caaggggggc catcacggtg gggaaaacgg
      661 ggtctgttgc gcgctgcaga acagggatgg atgctgcaag cggtatcgat ggggcctgga
      721 gaggcctaga attgcggggg aaagaggatt tgccaggttg aaggctggaa agcgcgaccg
      781 cacgagaggt tcgagcatcg aagatcgggt ctttgcatgg attcaccagg agcgcaggtg
      841 cgatgatcga tctttatacg tacaataccc ctaacggccg caaggtatcg attgctctgg
      901 aagagctagg gcttccctac cgggttcata tcgtggatat tctggcggga cagcagtttt
      961 caccggagtt tctcgccata tctccgaata ataagatccc ggcgattgtg gattcggaag
     1021 ggccggaagg aaagaccgtc cacctcttcg aatccggcgc gatcttgctg tatcttgctg
     1081 aaaagacagg gaaattaatt ccagcggacc cgatggggcg gttgtcgacg tggcaatggc
     1141 tattttggca agtggggggc gtggggccga tgttcgggca agccggccat ttcgttcatt
     1201 atgcgaaaga aaaaatccct tatgcgatcg agcgttacac gtcggaagtg aatcgattgc
     1261 taaaggtgct cgagcagcag cttgcgaaac agggccccta tctggtgggt gagacgtata
     1321 gcatcgcgga tattgcggtg gccccctggg tcgatggcct gtctttttat ggtttggccg
     1381 aacttctcga gccccatgag catattcggc gctggttgga gactttcaag gcgcggcctg
     1441 ccgtacaaaa agggtggaac gtatgagcgg gactggggcg tgagtttcgt gtttgggctg
     1501 gaattttctt tcaagtagaa ccgacgttcc ctcgcgcgga tctcctgaat cttttcgagt
     1561 agttcgtcga agtagtcttt gccaaaacgc ttgttcagct cgagccgctc gtcatcgagg
     1621 acgaatccct tgatgatgaa ctcgcgcaag gtctgggtcg cccagatgcg gaattgggtg
     1681 gcctggctgc tgttgacgcg gtagcccacc gaaatgatgg cgtcgagatg atcatgctcg
     1741 atttcacgcc ttacctcgcg atttccctcc cgttgaactc ttagaatttt tcgaagagtt
     1801 gactcctgct caagttcacc cgatgcataa atctctttca gatgctagct tatcgtgaaa
     1861 acctcgacgc ccaatagctt tgccatgcgg cgctggtcga gccaaaaggt ctcggattcc
     1921 taaagtattt caacgcgtac ggcgtcgttg gcggttcggt acaccatgat ttcgtcttgc
     1981 ttcgggccat cgacggtcat agtcctgggc tctcgaggac gggctggtgt tggaacgagg
     2041 tttatccctc acagcacgcg gattgcttgc atgaaacacg gatttttcgg ggtgtccggc
     2101 cccccgctgc gcatctttca tgaattttaa aatactcctc acccgtcacc caccatcgca
     2161 agatgcaaca gcggatacgc tcgcccttcg ctatcggttt cgctacgtcc ggtgggaaca
     2221 aatccgcaat gaacataaaa cccaacggcc ttcgggtttt gcgcattgac atccacggtc
     2281 agcggtccat cccccgccat gacgtgctcc aatagcttgc gacccatacc aaggcccatg
     2341 accagggggt ccaaaaacag catctcgata tgccgaccat tcataccgat aaaaccaaca
     2401 ggcctgtcgt cacccgagca cgcaacataa atctccacgt ttgggaaata aacggtagca
     2461 agcttttgtt cgatatccct aaaatcctct tcctttagaa agtcatgggt tgcacgcacc
     2521 gaacgacgcc aaatttccac caaagcttcg gtgtcatcgt tgcgaaaagg gcgaatgtcg
     2581 aagggcatgg taatttctga atgcattttt ttgccggacc ttgcgggaaa tcaaccgctg
     2641 tcggaaatcg ggttcccatg cgtgtcgaaa cggatgatcc gccgctgcgg attccctttt
     2701 ttcggcccca gcaaccattc gaccgtcagc gtcaggtctc gatccgaccc cttgattccc
     2761 gcggccgcca gttccgtatt cgcgcgacaa ctcgctgttt tcagcctgaa tgtctctatg
     2821 ggccgctgtc ctttgagatt gacgacacgc aaaaactgct cttcgccggc ctggcaatgc
     2881 gacatgggac cggccacgga cgggttggtg tccatcacca gcagggccgc cttgcctatt
     2941 tggcccatat tctccactcg cagcggcatc gctccagggg caacgtcatc atcgcgttcc
     3001 agccgcaaga cgccaatctc ccccgtgggg ccgtcgaagc gaatgccagg cgcgccgtcg
     3061 ccagcggcca cagcttcgat attgacgaca aatgcggcgt tgggtctgac tggttccgca
     3121 ggtctgcccg cgcaaccgac aaccgctgac acaagggcta gcgccaccca caaggggggc
     3181 ttgcgtggcg tggtgtgact tgcccacggc ttcgagagcg tcgtcaagtc gtagctaaaa
     3241 acagctatgg aacaaggata tacccgtttc ctttcgtccg acaatggcat tgtcttactg
     3301 cactccaccg tgttcccggc atcgtcagga cagggcttcc gtccatcgtg gcagttcgcc
     3361 aggggcaata ccttccaggg gtctgcgcag agcatcgact tcaatcaggt aaagtgcggc
     3421 aggacagtgc cgctgagcac ggaatccatc gaatattgcg cctttttcga ataacacgtc
     3481 gttttttgcc gaggtgcgca cggccaatcg catgtcagtg aacgccgtgc cgcttcgctt
     3541 gctcaaaagc cagaaccaga ttcacccgag actgcgagaa catttgggta acgagcgtca
     3601 tgcccatcaa ccacgcgact tactgccttc ccccaggagc gaactccgcg caatcgacct
     3661 gcacatcctg ggaaatcgaa acctcgcaac gccgataggc aagccttcgc cccccaacct
     3721 gttcgaaata caccagtgtg gctttgccgc tttccttgct caaacgcggc gcctcgagcc
     3781 cttcccggcg cgaaatggac ttcaaaagca cctcatcgag aaccgtaccg cggctgcgtt
     3841 gaatacaagc cgcaatttcg gccaaatctc gggctttggt cgtccctacc ccgacaatat
     3901 ccagcgtatg atagatttcc cccaaaacac ccatgtcatt tttctccacg accccacggg
     3961 ttgcatgcac cgcatagagt tctcgaggca aatcccgggc ttcgagacaa tccacggaaa
     4021 aaaactgcca atccgccacg gaaaacaaaa ggggggtgat ggaccactct accccggaac
     4081 accgcgtttt cagatggttt tgcagcgagc ggtaagcggg gctcggagct ttgggatctg
     4141 gcaaataccg cttgaatgcc gagtctccca caaccgtcga cgacttcatg gtcacctccg
     4201 atgggcgatg ttccgagggt tgatgttgcg atgggacagg tgttgggtct cccgggccca
     4261 tgacctccgc aggaggcgcc ttgcaacctc cgatggtccc ggaaaccata catagcaagc
     4321 tgcccagggc aatcgaggcc ttttgcatct ttggtgcgtg tagtgcaacc tggtccagga
     4381 cgcaagcaga cggatgccat cgcggcatcg cctatgtaga acgtgtttcc aagaaacaga
     4441 atataggatg ggtaggtcaa tcagccgtca aaacgccaca aaagaagggg gggtgaggcg
     4501 ccaagggtac ggaaaatcac acggattgtc cgcataacgt acgggatggt gagccggata
     4561 ccctctgaca gatcccctca tttattcctg aaatggaaag gggtacgatc aggggatccc
     4621 ccggctttga ttttgacctg gtccttgcgt cctggtgggc catccattat aaaacatcaa
     4681 taaata
//
LOCUS       DAOSOR010000477         4684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_18330_length_4684_cov_4.131778, whole genome shotgun sequence.
ACCESSION   DAOSOR010000477 DAOSOR010000000
VERSION     DAOSOR010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18330_length_4684_cov_4.131778"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1259
                     /locus_tag="PK710_20005"
     CDS             <1..1259
                     /locus_tag="PK710_20005"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT12071.1"
                     /translation="AGVTALSDHVVRITLREPDATLLPILTLGITAPVCRSAGYEYDP
                     QFDQIACGAGPFRLASWQGRDVLELKRHESYHDASHVHLSGIRWMFGVPSTSQRYRFE
                     RGELDIVHELTAADSIAFRADPRWAPLGSWSRPRSTRGIFMNTEMPPFHDVNVRRAIA
                     LAIDRNQIASLRAGHLVAANGMIPSGVVGYDPSFSGQQHDLSQALQLMKRAGWPYDPS
                     TDTGGYPHPIDYYCAADSFDVAVAEIFQQQLQRIGIRLRIKAVSWAAYLSVTGRRQQA
                     AMGADGWSADFDDPSDFFDPLFSSEAIQQEESQNRAFYNNPRLDSLLARARREMQPNE
                     RKKLYHLADGIVRDEAPWATVYGFRYYDVHQGYVQGYTPHPHAYLDVASVWLSVSQRT
                     LAARARKAPPLSIASLAYRLCSGRGR"
     gene            1256..2203
                     /locus_tag="PK710_20010"
     CDS             1256..2203
                     /locus_tag="PK710_20010"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT12072.1"
                     /translation="MMRRMIRRIGTSLFVVWFVLTLTFLIHHALPSDPARAMAGLQAR
                     PADVAELRIQLGLDQPLWKQYSRYMGRLVRGDLGQSYQRHTAVATILKERAFNTLMLA
                     LAATTLQVFFGTLAGMSVAFRRFRWIDHSVVFFTLVGISIPTFLSGLILQYWLAYRWR
                     LVAFDGFGATLTEQLHSLILPALTLGSVGAAFYARYVRDEVRGILHEDYMRTARAKGL
                     PTWRIMFVHGLRNALIPLITLLGMDLGTMMGGAVVTEKLFRWPGIGALTVDAILARDG
                     PVVMGIVVVTSVSIVIANLFVDGLYVIIDPRIRHPAETA"
     gene            complement(2254..4419)
                     /locus_tag="PK710_20015"
     CDS             complement(2254..4419)
                     /locus_tag="PK710_20015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT12073.1"
                     /translation="MAKLQLLLVDQDPNSRKVLEVSLKKAGYTVTVSDDGLDALAKIE
                     LSTPDLVLTDTSLSGIDGFALVKRMKENPAWATIPVVFLTSRNSVEDKVRGFELGVED
                     YLTKPIFVRELMARINMLLNRRAKERMAARQTTSGRTRFTGSILDMGVVDLLQTFEIS
                     RKSGTLHITYDNYEAVLLFRDGQLVHATLGHLQGPEAVYRTLVWNKGEFAIEFGNVNA
                     EDNVGVSTQGLLMEGMRRVDEWTRLQEQLPSLSVQLDVDAQQVIDRLHEIPDEVNKIL
                     RLFDGGRAIKDVLDESPFDDLSTLGTVSKLYFEGILVLADGEGNGAQVSESTPKYSVR
                     GEDAEPLIDEELDGAFLALASMSSSPPSMEANVDAPEATLEKRETLPVPAAPIDHADA
                     VEPPALSLPRSQDPLAKTNPGMPVIKPPKNAAEDDQASPVTKRASSLNDSNPDVEAKP
                     SPDIEENSSFPLSVDDSSFESDGIDHEPQEEQLQAWSKELEEDDEEEKNPDASRHRLS
                     KRAFEDEREDRDELEQEQGQASIETGGVNRKAAASRAAAVAIGALSVIAIFGIWRLQR
                     ESVPNRTLSSLSTAKPAVASSNVTPMNFSNRDAAFDASSSQQKVSSPLMPEAAAHDEP
                     KVDAEPMDAMKPESGEPVSKNLIFQAQRALERGRVNEAIAMAIDYTNAYPTDAFGWLV
                     LGAAYDQAGRRTEAREVYRTCASKAKGAYIGECRALGGQ"
BASE COUNT          954 a         1304 c         1297 g         1129 t
ORIGIN      
        1 tggctggtgt cacagcgctt tctgatcacg tggtccgcat caccttacga gagccggatg
       61 ccaccttgtt gcccattctt acgctaggaa ttaccgcccc cgtctgtcgt tccgcgggat
      121 acgaatacga tccccaattc gatcaaatcg cttgtggtgc gggcccgttt cgtcttgctt
      181 cgtggcaagg gcgagacgtg ctcgaactca aacgtcacga gtcgtatcac gacgcttcgc
      241 atgttcatct ttctggcatt cgttggatgt tcggcgtccc ttccacatcc caacggtatc
      301 gattcgagcg cggagaactc gatattgtcc acgaattgac cgcagccgat tcgatagcct
      361 ttcgtgcaga tccgaggtgg gctccgttgg gctcgtggag tcgtccacgc tccacacgcg
      421 gtattttcat gaacacggag atgcctcctt tccatgatgt gaacgtccgt cgcgccatag
      481 ctcttgccat cgacagaaat cagattgcca gtcttcgagc cggtcatctc gttgcagcca
      541 acggaatgat tccgtctgga gtcgtgggat acgatccatc cttttcggga cagcagcatg
      601 atttgtcgca ggctcttcaa ttgatgaagc gcgctggatg gccgtacgat ccctcgaccg
      661 acacgggcgg atacccacac ccgatcgact attattgtgc ggcggattcc tttgatgtcg
      721 cggtagccga gatattccaa caacagctcc agcgcatcgg catccgcctc cgcatcaaag
      781 ccgtttcttg ggcggcttat ctatccgtta ccggacgtcg ccaacaagct gccatgggag
      841 ccgatggctg gtcagcggat ttcgacgatc caagtgattt ttttgatcct ttgttttctt
      901 ccgaggccat ccaacaagaa gaatctcaaa accgcgcgtt ttacaataac cctcgactcg
      961 attcgctgct cgctcgggct cgccgggaaa tgcagcccaa cgaacgcaaa aaactgtacc
     1021 acctggctga tggcatcgta cgcgacgaag ctccctgggc caccgtatac ggattccgct
     1081 attacgatgt gcatcaggga tacgtgcaag gctacacacc acatccccat gcctacctcg
     1141 atgtggcctc cgtgtggctc agtgtctccc agcgcactct cgcagcacga gcgagaaaag
     1201 cacctcccct aagtatcgct tctcttgcct accgcctttg ttctgggagg ggacgatgat
     1261 gcgtcgaatg attcgacgga ttggaacgtc gctgtttgtc gtgtggttcg tattgaccct
     1321 tacgtttttg attcaccatg cccttccgtc ggatcccgct cgcgccatgg ccggtctaca
     1381 agctcgtccg gcagacgtcg ctgaattacg gatccaactc ggtctggacc aaccgctgtg
     1441 gaagcaatat agccgctata tggggcgtct ggttcgaggc gatctcggac agagctatca
     1501 aaggcatacc gccgtggcga cgattctcaa agagcgcgca ttcaatacgc tcatgctggc
     1561 tctcgcggca accacgctgc aagtgttctt tggaacgctc gctggaatga gcgtggcgtt
     1621 tcgacgtttc cgatggatcg accacagcgt tgtcttcttc acactcgtag gaatcagcat
     1681 tcccacattt ttgtctgggc tgattctaca gtattggctt gcctatcggt ggcggctcgt
     1741 tgcattcgat ggattcggcg caacgctcac cgagcaacta cattcgctca tcctccctgc
     1801 actcacgcta ggatcagtgg gagctgcttt ctatgcgcgg tacgttcgcg acgaggtgcg
     1861 tggcatcctc cacgaagact acatgaggac agctagagcc aaaggcttac ccacatggcg
     1921 aatcatgttc gtccatggtt tacgaaatgc actcattcca ttgatcacac tccttggaat
     1981 ggatctcggc acaatgatgg gaggtgccgt cgttaccgaa aaactctttc gttggcctgg
     2041 tatcggggca ttgacggtag atgccatcct ggcgcgagac ggccctgtgg tcatgggcat
     2101 cgttgtcgta acctcagtga gtatcgttat cgccaacctc ttcgtcgatg ggttgtacgt
     2161 cattatcgat ccgcgaatac ggcatcccgc ggaaacggca taacaatcat cgagcgagtt
     2221 ggtattggag ctttcgtgtg ttcgcccgta ccgtcattgc ccccccaacg ctcggcattc
     2281 tccgatatag gctcctttgg ccttgctggc acacgttcga tacacctcgc gcgcttcggt
     2341 ccgacgcccc gcctggtcat acgctgcgcc aagaaccaac catccaaaag catccgttgg
     2401 ataggcgttc gtatagtcga tagccattgc gatcgcctcg ttcacccgac cgcgttcaag
     2461 ggctcgctgc gcttgaaaaa tgaggttttt ggagacaggc tccccggatt cgggtttcat
     2521 cgcatccatc ggttcggcat ccaccttcgg ctcgtcatgc gcggcagcct ccggcatgag
     2581 cggcgacgat accttctgct gcgatgaaga tgcatcgaaa gctgcatcgc ggttggagaa
     2641 gttcatgggc gtgacattcg atgacgcaac cgccggtttc gctgtcgaca agctcgaaag
     2701 tgtgcggttc ggcaccgatt cgcgttgaag cctccaaatg ccaaagatcg ctatgaccga
     2761 caaagcgccg atcgccacgg cggcagcccg tgaggccgcg gcttttctat tcaccccacc
     2821 ggtttcgatc gacgcttgcc cttgttcttg ctcgagttcg tcacggtctt ctcgttcatc
     2881 ctcgaaagcg cgtttgctca accgatgacg tgaagcgtca ggattttttt cctcctcgtc
     2941 atcctcctcc agttctttcg accaagcctg tagttgttct tcctgcggct catggtcaat
     3001 tccatcggat tcgaaggatg agtcatccac ggacaatggg aacgacgaat tctcctcgat
     3061 gtctggtgat ggcttcgctt cgacatcggg gttcgagtcg ttcagcgaag aggctctttt
     3121 cgtaactgga gacgcttgat catcttctgc cgcatttttc ggaggtttga tgactggcat
     3181 acccggattg gttttcgcca gcgggtcttg acttcgaggc agagacaagg ctggaggctc
     3241 tacagcatca gcatggtcga tcggagccgc gggaaccggc aacgtctccc ttttttcgag
     3301 ggtagcttca ggtgcatcca cgtttgcctc catcgacggc ggcgatgaag acatgcttgc
     3361 aagggccagg aaagcaccat ccagttcttc gtcgatgaga ggctcggcat cctcgccccg
     3421 aacagaatat ttaggtgtac tttcacttac ttgcgcaccg ttcccctcac cgtccgcaag
     3481 cacgagaatg ccttcgaagt acaatttcga taccgtgccg agggtcgaaa ggtcatcgaa
     3541 cggagactcg tcgaggacat ccttgatcgc gcgtccacca tcgaagagac gaaggatttt
     3601 gttcacctca tcgggtatct catggaggcg atcgatgact tgctgagcat cgacgtcgag
     3661 ttgcacggaa agggagggaa gctgttcttg cagacgcgtc cattcgtcga cgcgacgcat
     3721 tccttccatg agcaagcctt gggttgaaac gccaacgttg tcttccgcgt tgacatttcc
     3781 gaattcaatg gcgaattccc ctttgttcca gaccagggtt cgatacacgg cctcagggcc
     3841 ttgcagatgt ccaagcgtgg cgtgaacgag ttgcccatcg cggaacaaca ggacggcttc
     3901 atagttgtcg taggtaatgt ggagagtgcc acttttcctt gaaatttcaa aggtttgcag
     3961 caagtccacc acacccatgt cgagaatcga gcccgtgaat cgagtccgtc cggaggtggt
     4021 ttgacgagcg gccatacgct ccttggctcg tcggttcagc aacatattga tgcgagccat
     4081 gagttcgcgc acgaagatgg gcttggtgag atagtcttcc accccgagtt cgaaccctcg
     4141 gactttgtct tcgacggagt tacgactggt gagaaagacc acgggaatgg tcgcccaagc
     4201 ggggttttct ttcatgcgtt tgaccaacgc aaagccatcg atgccggaca agctcgtgtc
     4261 cgtaagaacg aggtccggcg tagaaagctc aattttggct aaagcgtcga gcccatcatc
     4321 ggaaacggta accgtatagc cagccttctt gaggcttact tcgaggacct tgcgggagtt
     4381 cgggtcctga tcgacgagga gaagctgtag tttggccacg gcgttggagg ggagaatgag
     4441 aggcaggcgg gcagtgtgtc aagagaatcg gcaaaaggcg gacagatggc ggattccaag
     4501 gggcggcttg tgggaatggc ccagcgggaa aagtacatgg ggtacgtcaa tgatgccgtg
     4561 gttcgacagg ctggacgggt tggtcgaggc gagggcagaa cgtcgtgaag tgaatctggc
     4621 ggggttattg cgcccacggg ttgataatct cggaagttgg gtcggcgtgt tttcggggcg
     4681 gtgt
//
LOCUS       DAOSOR010000478         4682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_18338_length_4682_cov_5.086233, whole genome shotgun sequence.
ACCESSION   DAOSOR010000478 DAOSOR010000000
VERSION     DAOSOR010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18338_length_4682_cov_5.086233"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PK710_20020"
     CDS             <1..369
                     /locus_tag="PK710_20020"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740158.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOT12074.1"
                     /translation="SLARVVGYAGVAQDPAWFTTAPAAAIDKTLTTLGLKTQDIDLFE
                     INEAFAVVTMHSIQACKLDPEIVNVHGGAVSLGHPIGCSGARITTTLLHAMLDRKVKR
                     GLATLCIGGGEALAVVLETI"
     gene            522..1718
                     /locus_tag="PK710_20025"
     CDS             522..1718
                     /locus_tag="PK710_20025"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT12075.1"
                     /translation="MRFPRLLAIVTLTSLTAVSACSKKNSPNGSDSTVGKHAIPAAQE
                     GKFNVAFVHVGPVGDGGWTYAQDQGRKYLLQQIPEVHTAHVESVAEGAESEQVIRALA
                     RKGFNLVVATSFGFMDSTETVAKEFPNTKFIHISGFKKNDTNFGNAFGAMEQMKYLAG
                     MIAGARAAQDGVPRLGYIAPYPIPEVVRLGNALMLGAKQTCPQCTMEIRWMNSWFDPG
                     KEQEAAESMFNAGVQVVLTGADTTGPIVVAGQKKKWAVGYDSNNACDADKAHCLTTPY
                     WNWGVFYVDAVKQMRAGSWKPSSWYGEADSGLLGLIGFEDGATPAPGVPESVIPVVRE
                     KLAAMKAGTFTRFDVFAGPIKDNRGKEVLPEGKRLTQEDLEGLPGCKVCMNWLAEGIL
                     GQLSPK"
     gene            1771..2817
                     /locus_tag="PK710_20030"
     CDS             1771..2817
                     /locus_tag="PK710_20030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT12076.1"
                     /translation="MVKRFPGIVANDHVDFTLRRGEVHALIGENGAGKTRLMNMLAGV
                     QRPEEGEIFVHDKAIQIRSHRDADPWGIAMVHQELMLAEALTVAENVALGLRNLKWQI
                     RCSSIERSIEEQSRVLGFDIDPRAYVWHLSIGQRQRVERLRALMRGVRILWLDEPAAL
                     LSPDEKVLDVHEVHACGDKGFEALRHISCKVFAGEILGVAGVSGNGQRELADVFRGMR
                     PTTSGRITLCGNDVTHATPARYLRAGLAYIPENRRTQEAVTSLSLAENLAQKTYRHPP
                     IAKRWGLWRKEMRSFARRLVDTFHVQAVSLQQPAGHLFSGNLQRLILARELSHPISLL
                     LAAQPTRGLDFAAS"
     gene            complement(3243..4178)
                     /locus_tag="PK710_20035"
     CDS             complement(3243..4178)
                     /locus_tag="PK710_20035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12077.1"
                     /translation="MVLRSKVPHDMILDPVISPDEFARCDCGATELWRPVLLADTGAR
                     ACMACGHVRVVHDEWSYPKNPHLDPEVIATRLLTIDDDVRPWLGAFPRLVASPPADSN
                     EPCWLPASLRVADAQMLLAVECELAAAPPLPRLGDRLRAAGIPAAPPRRDMRWEFTYW
                     LDVHDALLLTDESPLGDVLRLASSQTPLAARIANEQLDCRAGLLDSLLGAFETGTPPR
                     NMVLPALRRLAATAPIEACTAVGSAVANRLSSLLDVPFDNEVEIEEIVLLLLALGPTA
                     AVAIEPLRRLEASELVRCRSLLRDGIEELRAVLAR"
BASE COUNT          997 a         1392 c         1412 g          881 t
ORIGIN      
        1 tctctcgcca gggtggtcgg gtatgccggc gtggcgcaag atccggcttg gttcacgacc
       61 gcgccggcgg ccgccatcga caagaccctg accaccctcg gtttgaaaac ccaagacatc
      121 gatctattcg aaatcaatga agctttcgcc gtcgtgacga tgcattcgat ccaggcctgc
      181 aaactcgacc cggaaatagt gaacgtgcat ggcggcgccg tttccctcgg tcacccgatc
      241 ggatgctccg gtgcgcgcat caccacgacg ttgctgcatg cgatgctcga ccgaaaagta
      301 aaacggggat tggcaacact gtgcatcggt ggaggcgaag cgctcgccgt cgttctggaa
      361 acgatctgaa aaacccgctt tcctacggtc attcccctac gttgccgtgc ctgtcatttt
      421 ttgtctggtg taggcttgcg cgggttcgat cgcaatcatc accggggccc ctcgaccacg
      481 ctatggttcg agaggggttc ccgacgcaag ggcatgaaac catgagattt ccaagactgc
      541 tcgccatcgt gacgctcaca tcgcttacgg cggtctccgc ttgcagcaaa aaaaattcac
      601 ccaacggatc cgactccacg gtgggcaaac atgccatccc ggccgcgcaa gaaggtaagt
      661 tcaacgtcgc cttcgtgcat gtcggccccg tgggtgacgg cggatggacg tacgctcagg
      721 atcagggtcg aaaatacctg ttgcaacaga tacccgaggt tcataccgca cacgtcgaaa
      781 gtgtggccga gggcgcggaa tccgaacaag tgatacgcgc cttggctcga aaaggtttca
      841 atctggtcgt cgccacttct ttcggattca tggacagcac cgaaaccgtc gccaaggaat
      901 ttccaaacac gaagttcatt cacatctccg gattcaagaa aaacgacacc aacttcggca
      961 atgcgttcgg cgccatggaa cagatgaaat acttggcagg catgattgcc ggagcccgcg
     1021 cggcacagga tggtgttcca cgactgggct acatcgctcc ttaccccatc ccggaagtgg
     1081 ttcgtctggg caacgcgttg atgttgggag caaaacaaac atgcccacaa tgcacgatgg
     1141 aaatccggtg gatgaattct tggttcgatc ctggaaaaga acaggaagcc gccgagtcca
     1201 tgttcaacgc gggcgtgcaa gtcgtgctta ccggcgccga taccaccggc cccatcgtcg
     1261 tggctggaca aaaaaagaaa tgggccgtcg gctatgattc caacaacgct tgcgatgccg
     1321 acaaagcgca ttgtttgacg acgccctatt ggaattgggg cgtgttttat gtcgatgccg
     1381 tcaaacaaat gcgcgcggga tcgtggaaac cgtcgtcgtg gtacggtgag gcggactcag
     1441 gccttctcgg gctcattgga ttcgaagacg gagccacgcc cgctcccggc gttcccgaaa
     1501 gcgtcatccc tgtggtacgc gagaagcttg ccgccatgaa ggcgggcacg ttcacccgct
     1561 tcgacgtttt tgccggaccc atcaaagaca accggggcaa ggaagtcctg ccggaaggaa
     1621 aacgtttgac gcaagaggac ttggaaggct tgccgggctg caaggtgtgt atgaattggc
     1681 ttgcggaagg catccttgga caattgtcgc cgaagtgaat cgtatcgccg catccgctga
     1741 gcccaaagac atgacgtgac caagcgcggc atggtcaagc gcttccccgg catcgtggcc
     1801 aacgatcacg tcgacttcac cctccgacgg ggggaggtac atgcgctcat cggagaaaac
     1861 ggagccggca aaacgaggct catgaacatg cttgcgggag tgcaacgccc cgaggaaggc
     1921 gagattttcg ttcatgacaa agctatccag atacggtcac atcgcgatgc ggacccatgg
     1981 gggatcgcca tggtgcatca ggaattgatg ctcgccgaag cgctcacggt tgcggaaaac
     2041 gttgcccttg gcttgcgaaa cctgaaatgg caaatccgct gctcgtccat cgaacggtcc
     2101 atcgaagaac aatcgcgcgt cctgggcttc gacatcgatc ctcgggctta cgtctggcat
     2161 ctatccatcg ggcaacgaca acgggtcgaa cggctgcggg ccctgatgcg aggcgtccgt
     2221 atcctgtggc tcgatgaacc cgccgccctg ctcagccccg acgaaaaggt actcgatgtg
     2281 catgaagtgc atgcttgtgg cgacaaggga ttcgaagcct tgcgtcacat ttcatgcaag
     2341 gtatttgcag gcgaaatcct gggtgttgcc ggagtatccg gaaatggtca gcgggagctg
     2401 gctgacgtgt tccgtggaat gcggcctacc acctcagggc gtatcaccct ttgcgggaac
     2461 gatgtgacgc atgccacgcc agcgcgctat ctgcgtgcag ggctggccta cattcccgaa
     2521 aaccggcgca cgcaagaggc cgtcaccagt ctttcgctag ccgaaaacct ggcgcaaaaa
     2581 acttatcgtc atcctcccat cgccaaacgt tggggcctgt ggcggaaaga aatgcgttcg
     2641 ttcgccaggc ggctcgtcga tacctttcac gtccaggcgg tctcgttgca gcaacccgcg
     2701 ggccatctgt tcagcggcaa cttgcaacga ttgatcctgg cgcgagaact gtcccatccc
     2761 atttccttgc tgttggcagc acaacctacc cgggggctag atttcgcggc gtcctgaccc
     2821 ccatgtgccc agtcacatta ccgtcaaatt ggatgatcgg tgcatgacaa ggacagatat
     2881 ggggcctcgt ggtgggaaga acgcggttgg cggagcgaat acacgtgact tgcttgatcg
     2941 tttgcgcctt gctcagctca tcgaccaaga gcggatggag gccagcatcg aaccgcgacc
     3001 tgcttcgtgg ctgttactgt atctacggcc ttgcgcgatt cacattgatt gcagggctct
     3061 gcgctacgag gtggtcaaga gggggagaac gacgagagcg tcatccctca cccttcgcct
     3121 cacggcgatc cacccgtttc ctcccgtcga ccaccgctcc tagcggtaca gaacccgctg
     3181 ccatccccgc tggctcgagt tcacctgccg gatgcccttc catcgttctt tcggttcact
     3241 tattacctcg cgagcacagc tcgaagctcc tcgatcccgt cccgaagcag gctgcgacag
     3301 cgcacgagtt cgctcgcctc gagtcgccgc aacggctcga tggcgaccgc tgcggtggga
     3361 ccgagcgcca aaagcagcag aacgatttcc tcgatctcca cttcgttatc gaagggcaca
     3421 tcgaggagac tcgataggcg gttcgcgacc gcgctgccga cggccgtgca ggcctcgatg
     3481 ggcgccgtgg cggcgaggcg acggagcgcc ggcaagacca tgttgcgggg cggcgtgccg
     3541 gtctcgaacg ccccgagaag cgaatcgagg agcccggcgc gacagtccaa ctgctcgttc
     3601 gcgatacgag ccgccagcgg agtctggctc gaggctagcc gaagcacatc gccaaggggg
     3661 ctttcgtcgg tgagtaacag cgcatcatgg acgtcaagcc aatacgtgaa ctcccagcgc
     3721 atatcgcgac ggggcggtgc cgcggggatc ccggctgcgc ggaggcggtc gccaagtcgc
     3781 ggcagtgggg gcgccgcggc gagttcgcac tcgacggcga gcagcatctg tgcatcggcc
     3841 actcgcaggc tcgccggcag ccagcagggc tcgttcgaat cggctggcgg actcgctacc
     3901 aggcgcggga aggcaccaag ccacggtcgg acatcgtcgt cgatcgtcag cagccgggtg
     3961 gcgatgactt cgggatcaag gtggggattc ttcgggtacg accattcgtc gtgaactaca
     4021 cgaacgtgac cacatgccat gcaagcgcgc gccccggtgt cggcaagcag cacgggccgc
     4081 cagagttcgg tcgctccgca gtcgcagcga gcgaattcat cgggcgagat gacggggtcg
     4141 aggatcatgt cgtgcggcac tttcgagcga agcaccaagc gggagccagc agcgactcct
     4201 ccaggacttt accccaccag aattcgagat ggcaaacggg acccggtacg cggtgacggc
     4261 gggggtgttt ggaggcttgg tgagacaacc gtccgtagcc gagttgccct ggcgaaaacc
     4321 atagctgcgc catcctgtcc ggcaaagacc attcgttgga cgcttctgtt gcccataggc
     4381 aaatcggtca gttttcgcaa atcaagacaa gttccggatc acgtcgcggg ggcggcgtaa
     4441 aggcagtcaa gacggaatca gggaggattc atggtgtcaa cgcgatcggt agacaaacca
     4501 atgcgtcgcc gccttttccc gcagcgcata acccactgcc ctgcgcgatc agcagctacg
     4561 ccacgaacaa gaccgggtcg aagcccggtt gtggggcagg agcgtgtgcc tcggtgatgc
     4621 tggcaatgac acgaagtctc ccgccgcaac gcggacattg cagcgaatct acgtcgaaca
     4681 ct
//
LOCUS       DAOSOR010000479         4667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_18420_length_4667_cov_3.111232, whole genome shotgun sequence.
ACCESSION   DAOSOR010000479 DAOSOR010000000
VERSION     DAOSOR010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18420_length_4667_cov_3.111232"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1278)
                     /locus_tag="PK710_20040"
     CDS             complement(<1..1278)
                     /locus_tag="PK710_20040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12078.1"
                     /translation="MKRFRSWSTHWVASLAVAWLGVGLFMPGCGASQAVPDMSARFYS
                     VHTAMQASGLTQMGGVSRGRLAASQSIKLPLELSAECLTVVAMGDEGVADVSLALKGV
                     DGKEVARDDMIGPDASMRYCPDQPGKYELELSMAKGSGGYAVSVWTGGVPTRRVEAAP
                     QGALTVEGGGSCEAPTILVAGQTYVGNTEDGRKMEEGSCGNTNARELVYRLDLVERQR
                     VSLDLRAEYDGVLYVRRGDCADPEAEVACNDDAPGGGRRSRVDEVLDAGTYYVFVDGY
                     GEEEGAFRLTVQLRPAGNAQNQCEEAPSIVLGTVVRGSLVDGVNNASATCGNDAKGPD
                     VPFRLDLASRSRVRIQHQASGYPPVVHVRSSCERPESEEGCSATGMAVGEATFTGELN
                     AGTHYVFADAAQLASGDYALTVQAADAVGGGAQG"
     gene            complement(1408..2835)
                     /locus_tag="PK710_20045"
     CDS             complement(1408..2835)
                     /locus_tag="PK710_20045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOT12079.1"
                     /translation="MARFEFKLPDIGEGVTEGEIVAWGDGVAVGARVEVDQTLAEVMT
                     DKATVTIPSPKAGVIVHLGGEIGSVLSVGAVLVVIETEGAAGVEVATAESAQRDALPK
                     PSKASEPMEIKGQTPVAPSPKPAPVAPSTQDDPSKTKEGPSATAAGSIPEVLPGMQEA
                     AAEKPLAMRSERPLAAPATRKLARELGVDLTQVAGSGPNGRVTQQDVQRFAEQKSAGT
                     SIVKPAVMPVSPMSISSPEGVGQALEERIPLRGMRKAIFHQMARSKHTAAHFTFVEEC
                     HVTALKALRERMRPLAEAEGVKLTFLPFIIKACVAALKRHPMLNAAYDEVTDEIVLRY
                     TYNIGVAAATEAGLMVPVVKGAENRSLMEISKEIQRLSEDAQAGKIARADLMGSTFTI
                     TSLGAVGGLFATPILNFPEVAILGVHQIKQKPVVVDGNIVIGDVMLLSLSFDHRIIDG
                     HVGAAFAYEVIRYLENPDKLMLEMG"
     gene            complement(2926..3894)
                     /locus_tag="PK710_20050"
     CDS             complement(2926..3894)
                     /locus_tag="PK710_20050"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOT12080.1"
                     /translation="MNMVQAINDALRLEMRREARVVVLGEDVGRLGGVFRVTQGLYDE
                     FGEDRVIDTPLSEAGIIGAAVGMAMYGLVPVPEIQFADFIFPAYDQIVSEVAKMRHKS
                     GNEYQSKMVIRSPVGGGIRGGHYHSQSPESLFIHVAGLKVVCPSNPYDAKGLLIASIR
                     DPDPVLFFEPKRLYRAAKGEVPAEAYEVPLTSAAVLRQGTDVTVVTWGAMVYEALAAA
                     QKAEEQGVSTEVIDLRTLWPVDIDTITASVEKTGRVVVVHEAPKTCGFGAEIVALLNE
                     RVFWHLEAPPKRVAGFDTPFPYTLEMEYLPLAHRILPAILESAHTV"
     gene            complement(3958..>4667)
                     /locus_tag="PK710_20055"
     CDS             complement(3958..>4667)
                     /locus_tag="PK710_20055"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOT12081.1"
                     /translation="FGSVSSPIGTQMTHAVGFAWGAKIRGEDIVTLSFFGEGSTSSNE
                     FHNAMNFAGVFHVPTVLFCRNNHWAISVPSERQSASATFAEKGTAYGVRFVRCDGNDV
                     LAVIRATRDAVERAAAGGGPTLVEALTYRMSGHSTSDDPTIYRSEQQLEPWRRRDPIE
                     RLRQHLGVLGVWDDAKEEKLATEIEAEIQAAVDNAEATEAPSLSSMFDDVFKDLPWHL
                     REQRDELLGGPRASGHH"
BASE COUNT         1021 a         1671 c         1034 g          941 t
ORIGIN      
        1 tccttgcgcg ccaccaccca cggcatccgc agcctgaacg gtcaaagcat aatcgcccga
       61 tgccaactgg gccgcatccg caaagacata atgagttccc gcattcaact ccccggtgaa
      121 cgtggcctcg cccaccgcca ttcccgtggc cgaacacccc tcctcacttt cgggtcgctc
      181 acaagatgat cgcacatgaa cgaccggcgg ataaccgctg gcttgatgct gaatgcgcac
      241 acgtgatcgc gaagccaaat caagacgaaa agggacatcc ggccccttcg cgtcatttcc
      301 acacgtggct gatgcgttgt tgacaccatc gaccaacgat ccgcgaacta ccgttcccaa
      361 cacaatcgac ggagcttctt cacattgatt ctgcgcattt cccgctggcc gaagctgcac
      421 cgtcaaccga aatgccccct cttcctcccc ataaccgtcc acaaatacat aatacgtccc
      481 agcatcaagc acttcatcta cgcgggaacg tcgccccccc ccaggagcat cgtcgttgca
      541 cgccacctca gcctccgggt ccgcgcaatc cccccgacgt acatacaaga ccccatcgta
      601 ttccgcgcga agatccaagg atacacgctg acgctccacc aaatcgagac gatacacaag
      661 ttccctggca ttggtgtttc cacacgaccc ctcttccatc tttcgaccgt cctccgtgtt
      721 ccccacgtag gtctgacctg ccacgagaat tgttggcgcc tcgcaggaac ctcctccctc
      781 cacggtcaaa gcgccttgcg gcgcagcctc cacacgacgc gtcggcaccc ccccagtcca
      841 cacggaaacc gcataccccc cagagccctt cgccatcgat aactccaact catacttgcc
      901 cggttgatca gggcaatatc gcatcgatgc atccggcccg atcatgtcgt cacgcgccac
      961 ctctttgcca tctaccccct tgagcgccaa cgacacgtcc gctacccctt catcccccat
     1021 ggctaccacg gtcagacact cggcagataa ttctaacgga agcttgatgc tctgggatgc
     1081 ggcaagacgt ccccggctca ctccccccat ctgcgtgaga ccactggcct gcatcgccgt
     1141 gtgaacagaa taaaacctcg cggacatgtc cggcaccgct tgcgatgctc cacatccagg
     1201 catgaacaac cccacgccca accatgcaac cgccaggctt gcaacccaat gtgtggacca
     1261 agagcgaaat cgtttcacac ccctgaggct acgtctcttc gccccataac ggtagaggat
     1321 gttgcgattt ctatcgttcc cgcctccgag caaaggcgag cggaaggtgc ttgtcaacgc
     1381 ttcgaaccag gaccgtgaac ggtaccatca gcccatttca agcatcagct tgtccgggtt
     1441 ttcaagatag cgaatcacct cgtacgcaaa ggccgcaccc acatggccgt caatgatccg
     1501 gtgatcaaac gacaagctca aaagcatcac atcaccgatc acgatatttc catccacgac
     1561 aacgggcttc tgcttgatct gatggacacc caaaatggcc acctccggga agttgagaat
     1621 cggagtggca aacaatcccc ccaccgcccc caacgaagtg atcgtaaacg tcgagcccat
     1681 caagtccgcg cgcgcaatct tacccgcctg ggcatcctcg gaaagtcttt gaatttcctt
     1741 ggatatttcc atcaagctgc ggttctcggc tcccttgacc accggaacca tcagccccgc
     1801 ttccgtcgct gccgccacac caatattgta ggtgtaccgc aacacaatct cgtcggtgac
     1861 ttcatcatac gccgcgttca acatcggatg acgcttcaac gccgccacgc aagccttgat
     1921 gatgaacggt aaaaacgtga gtttgacccc ctctgcctcc gccaaaggcc gcatcctctc
     1981 acgcaaagcc ttgagcgcgg tgacatggca ctcctccaca aaggtaaaat gcgccgccgt
     2041 atgcttcgac ctcgccattt gatggaagat cgcttttcgc atgccccgca gcggaatacg
     2101 ctcctccaac gcttgcccca caccctccgg cgaagaaatg ctcatcggtg ataccggcat
     2161 caccgcgggt ttgacaatgc ttgtcccggc cgacttctgc tccgcaaacc tctgcacatc
     2221 ctgctgcgtc actcgaccat tcggcccgga acctgccacc tgcgtcagat cgactccaag
     2281 ctctcgcgcc aacttacgtg tcgccggcgc ggcaagagga cgctccgaac gcatcgccaa
     2341 tggcttctct gcggctgcct cctgcattcc cggaagtacc tccggtatcg agcccgctgc
     2401 cgtagccgag ggtccctcct ttgtcttgga aggatcatcc tgtgtcgacg gcgctactgg
     2461 agccggtttc ggcgatgggg caacgggcgt ttgtcctttt atttccatgg gttccgatgc
     2521 tttcgacggt ttgggcaacg catcgcgctg cgccgactcc gccgttgcca cttcgactcc
     2581 cgccgcacct tcggtctcta tcaccaccag aaccgccccg accgaaagca cagaaccaat
     2641 ctcccccccc aaatgcacaa tcaccccagc cttcggactc ggtatcgtca ccgtcgcctt
     2701 gtccgtcatc acctccgcca gggtctgatc cacctcgacc cgcgcgccca ccgctactcc
     2761 gtcgccccat gctacgattt ccccctccgt aacaccctct ccgatgtccg gaagcttgaa
     2821 ttcaaaacgc gccatgctca gcctctccgt ggttcggtcc tcgtcatctg catcaaagcc
     2881 agcacctgcc gcctcgacgc tgtcatccac tcgttgatac tcgactcaaa cggtatgagc
     2941 gctttccaga atcgcaggca ggatccggtg cgccagggga aggtactcca tctcgagcgt
     3001 gtatgggaaa ggagtatcga aaccagccac acgttttgga ggcgcttcga gatgccaaaa
     3061 cacccgctca ttgaggagcg ctacgatttc tgctccaaat ccacaggttt tcggcgcttc
     3121 gtggaccacc acgaccctgc ctgttttttc gacgctggcc gtgatcgtat cgatatccac
     3181 cggccaaagt gttcttaaat caatgacttc cgtagatacc ccctgctcct cagccttctg
     3241 cgccgccgcc aacgcctcat acaccatcgc tccccacgtc acgaccgtaa catccgttcc
     3301 ctgcctgagc actgccgccg atgtcaaggg cacctcatac gcctccgcgg gaacctcacc
     3361 cttggcagca cgatacagcc gcttcggctc aaagaatagt accgggtccg ggtcgcggat
     3421 cgacgcaatc aacaacccct tggcatcata cggattcgat ggacagacaa ccttcaagcc
     3481 cgccacgtga atgaatagcg actccgggga ttgggaatga taatgccctc ctcgtattcc
     3541 ccctcccacg ggcgatcgaa tgaccatttt cgactggtat tcattcccgg acttatgacg
     3601 catcttcgcg acttcactta cgatttggtc ataggcggga aaaatgaagt ccgcgaattg
     3661 tatctccgga accggaacga gtccgtacat cgccatccct acggccgccc caataatccc
     3721 cgcttccgaa agcggcgtgt caatcacccg atcttcccca aactcgtcat acaacccttg
     3781 cgtgacacga aatacaccgc cgagtcttcc cacatcctcc ccaagaacca cgacacgcgc
     3841 ctcccgtcgc atctccaacc gaagcgcgtc attgatggct tgaaccatat tcatacgagg
     3901 catcgatcgc tgccctcctg tatgcaagac ctctacggcc cttgtctatc cgctttgtca
     3961 atgatgccct gacgctctcg gtccacccag cagctcgtcc cgctgctcac gaagatgcca
     4021 cggcaagtct ttgaatacat cgtcgaacat cgacgacaac gacggcgctt ccgtcgcctc
     4081 cgcattgtcg accgctgcct gtatctctgc ctcaatctcg gtcgccaatt tctcttcctt
     4141 cgcgtcatcc catacaccaa gcacacccaa atgctgccgc agtcgctcga tgggatcccg
     4201 tcgacgccaa ggttcgagtt gctgctccga acgatatatc gtcggatcat cactcgtcga
     4261 atgccccgac atacgatacg tcaacgcctc caccagcgtc ggccctcccc ccgcggcagc
     4321 acgctctacc gcatccctcg tggcgcgaat caccgccaat acatcattgc cgtcacatcg
     4381 gacgaaacgc accccatagg cagttccctt ctccgcaaac gtggcagaag ccgattggcg
     4441 ctccgacggc acactgatgg cccaatgatt gttacgacaa aacagaaccg tgggcacatg
     4501 aaacacacct gcaaaattca ttgcattgtg aaactcattg ctgctggtcg agccctcccc
     4561 gaaaaacgac agggtcacga tatcctcgcc ccgtattttc gcaccccacg caaaaccaac
     4621 cgcgtgcgtc atctgcgtgc caatgggcga actcaccgat ccgaact
//
LOCUS       DAOSOR010000480         4620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_18677_length_4620_cov_4.672070, whole genome shotgun sequence.
ACCESSION   DAOSOR010000480 DAOSOR010000000
VERSION     DAOSOR010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18677_length_4620_cov_4.672070"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PK710_20060"
     CDS             complement(<1..556)
                     /locus_tag="PK710_20060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12082.1"
                     /translation="MDPKSLLIRSSFAAAAITFAAPGWAGPVDLLEADTQAFVQDSGA
                     MDVIYSLRYQDNEGRSAIKSIGQFYEPVAFTRGILHAGNNTIAVTMSPNGGGYYKASF
                     DSAVPRGTYTLELHYRSDKRFAEPTVSNDGKNLLAVWFNPVRWTMPIEKSIVKLVLPL
                     PLEGNLQRPEDITPAMVDGLGVITD"
     gene            1258..3393
                     /locus_tag="PK710_20065"
     CDS             1258..3393
                     /locus_tag="PK710_20065"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOT12083.1"
                     /translation="MKTNRWLVAVGCAIVLVMGGVAGCGDDDDDSGTKPSTGGSGGAG
                     GTGGTAGTAGQAGSGASAGEGGAAQDRKLTLLFTSDEHSDFLGYSPERDDYPLPTQAG
                     SGSIRAGIARRATLLENLRNEAVAADVSSITVSAGDNAMGTLIQMVRGSAALEWRLMH
                     KLGYDLTTLGNHDFDLGLPNLVSALKTAQALGELPGIVASNIHFSASDPADDELEALY
                     STDPAAKAAMHSYRVIETSDGIRVGFIGIMGVDASAVAPFKTPVTFSEVHVDPSDANN
                     TDKVLPHLYADLQPVVDTLRNDEKVDLVVALSHAGLAPDLPELSEDDKIAAHVAGIDV
                     IISGHAHESSATPVIVKNEVTGKEVIVLNGGSRGQFVGRIDLTIPADPSQSVRFDTGT
                     QALVPVDDTIVPDTQYASMLEEAIETIESSGDIGGKTALEHLISRSLGTDVKNDASQA
                     GDLYFYPLATMSFDMKGSRPLAFLTADAHLHMLDTLGMTADFAIQAGGTIRTELLKGK
                     TGAITVGDAFRIVPLGTSPFDGTIGYPLVRVRIPIGAVRLLFEFTSGRGPGASSFDLT
                     GGGIVAEYDCSREPITTVAQAFVPENGRVMKLWIDSDHSDGIEQFDTLVWDRANPPAS
                     VLGGDKYVMVTTNYIAQVMADSMIPLYDENDKIIKPNDAVVFRSDHTEVKELESFFAY
                     IHEQASIPDRYDPSSKNATSRFEGMAFCP"
     gene            complement(3549..>4620)
                     /locus_tag="PK710_20070"
     CDS             complement(3549..>4620)
                     /locus_tag="PK710_20070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12084.1"
                     /translation="YPQDKPMTVRYHHATFVATNDLLSMKVDVPTLFCVFASLILHGV
                     MLGMGLRTVTGIEDVEHVFSPDEWTGNAIDINVEEISKPVEFTNVSRVSTNPPAVVPP
                     PSPATVLAPRPTANLPKEPEPPIVVKPAPSPSPISTLAAPSISSNPSALPAPTASVKD
                     ESFELDGGADANALANRIDGGQEDGLWAGRSDGGQVAGTSSFGAAGSRRVRNLATAFA
                     RAMPIANTTDPVWETLPLGNAGTLEVQVDVDGEGKIVSAKPTDPAQRPNHLTRLVERT
                     VILLRSGRFALRGMSTEEGSETFLLEARIEQIELTPSDDPSQAGPYALGFSPPRKSEP
                     GYATFTLRSGRRIHISISLKEP"
BASE COUNT         1022 a         1265 c         1324 g         1009 t
ORIGIN      
        1 cgtccgtgat caccccgagc ccgtcgacca tggcgggcgt gatatcctcc ggacgctgta
       61 gattcccttc caaaggcaga gggagcacca gtttgacgat agatttctcg ataggcatcg
      121 tccagcgcac ggggttgaac cagacggcaa gcagattttt gccatcgttc gacaccgtgg
      181 gctcggcgaa ccttttgtca cttcgataat gcagttcaag cgtataggtg ccgcgcggaa
      241 cggcggagtc gaaagacgct ttgtagtagc cgccgccatt gggagacatg gttacagcta
      301 ttgtattatt tccagcatgg aggattccgc gggtaaaagc gacgggttca tagaattgtc
      361 caatggactt gatggccgag cgcccttcgt tgtcctgata acgaagcgaa tagatgacat
      421 ccatggcgcc gctgtcctga acgaaagcct gggtatccgc ttcgagcagg tcgacgggac
      481 ctgcccaacc tggtgcagca aacgtgatcg ccgccgcggc aaaagagctt cggatcagga
      541 gtgattttgg atccatcgta acctcgagga cagttcctgg ggacggacga gaggatatca
      601 gtgaggaacc gttatcttca atacccttgt gagttccgtg cggtcgacac ggtgctcatt
      661 gggacctggg ttcagacgag cgacggaatc gcaaggattt tagattatcc ctgaaaaatc
      721 cgaagcttac ggttttctat cgaaatcgaa gcgtcgtttt tcatcgctac aagaaaaggc
      781 tcgatgacta atgaaacagg ttaatcataa ggctaccgca agagggtgaa tggtagggga
      841 aagtgccaat gtccggggag cggcatcggt gtccggcttt ttgcgtcgga gtcatgacga
      901 tcgcaccgac cctcaggccg agggcggagc cacaagctag aatacttcag tccttcaagg
      961 attcacagcg atgacgacct catggtttgc ataatcgaaa cgaataaccg gaatttttct
     1021 acaagaaatc ccgtgtcgcc cttgcaggat cctgaaagcc gtggtttctt tgtccggtga
     1081 acattgtcga cgattgggtc gtggtatgcg gtcttactct tcgaaacggc acgacgtaag
     1141 gagtattcaa gaaatgaccc ttgcttttca aaaaataccg tcgtcggcat gcttagcgcc
     1201 gcatagcgat tgcggcgggg taggtgtcgc gcctgcctcg atggaaaagg agtacgcatg
     1261 aaaacaaatc gttggcttgt ggccgtaggg tgcgcaatcg tcttggtgat ggggggagta
     1321 gcgggatgcg gtgatgatga cgatgactcg ggaacgaagc cgagcaccgg agggtctgga
     1381 ggggccgggg gcaccggggg gaccgcaggc accgcggggc aggccggctc gggcgctagc
     1441 gcgggagaag ggggcgcggc ccaagaccga aaacttacct tgttgttcac gtccgatgaa
     1501 cactccgatt tcctcggcta cagccctgag cgcgatgatt atccgttgcc cacccaagcg
     1561 gggtcgggaa gcattcgcgc gggcatcgct cgtcgcgcca cccttctcga gaaccttcgc
     1621 aatgaagctg tcgccgcgga tgtatcatcc attacggtga gcgcggggga taacgcaatg
     1681 ggaacgctga tccaaatggt gcgcggctcg gcggccctcg aatggcgcct catgcataaa
     1741 ctcggttatg atctgacgac tcttgggaat catgacttcg acttggggct gcccaacctc
     1801 gtgtcggctc tgaaaaccgc ccaagctctc ggggaactgc ccggaatcgt ggcttccaat
     1861 atccacttct ccgcgtccga ccccgccgat gatgaattgg aagcgctgta ttcgaccgat
     1921 ccggccgcca aggccgccat gcattcgtat cgcgtgatcg agacaagtga tggaattcgt
     1981 gtcggtttta ttggaatcat gggcgtggat gcctcggcgg ttgccccctt caaaaccccc
     2041 gtcacttttt ctgaagtcca tgtcgaccca agcgatgcca acaataccga caaagtcttg
     2101 ccgcaccttt atgccgacct acaacccgtc gtcgatacct tgcggaacga cgaaaaggtt
     2161 gatctcgtgg ttgccctgtc tcatgcggga cttgcaccag acctgccgga actgagcgaa
     2221 gacgacaaaa tcgcggctca tgtggcgggc atcgacgtca ttatcagtgg tcacgctcat
     2281 gaatcatcgg ccacgcccgt aatagtcaaa aatgaagtga cgggcaaaga ggtcatcgtg
     2341 ctcaatggcg gctcgcgcgg tcaattcgtg gggcgcattg atttgaccat tcccgctgac
     2401 ccttcccaat cggttcgttt cgataccggt acgcaagcgt tggttccggt ggacgacacc
     2461 atcgttcccg atacccaata cgcttccatg ctcgaagaag ccatcgaaac catcgagtct
     2521 tccggagata tcgggggcaa gacggcattg gagcacttga tcagcaggtc gttgggaacc
     2581 gacgtcaaga acgatgcatc gcaagccggg gacctctatt tctatccgct ggcgaccatg
     2641 tcgtttgaca tgaaaggttc gcggcctttg gcatttctca cggcggatgc gcatctgcac
     2701 atgctcgaca cgttgggaat gacggccgat ttcgccattc aggccggcgg aacgattcgc
     2761 accgaactgc tgaaaggaaa aaccggcgcc atcacggtag gtgatgcatt ccgtatcgtg
     2821 cccttgggaa cgagcccctt cgatggcacg attggttatc ctttggtgcg cgtgcgcatt
     2881 cccatcggtg ccgttcggct attgttcgag tttacgagcg gtcgtggccc aggggccagc
     2941 tcgttcgatc tgaccggtgg tggcatcgtg gcggagtatg attgttcgag agagcccatc
     3001 accacggtgg cgcaggcatt cgtgcccgaa aacggccgcg tcatgaagtt gtggattgat
     3061 tccgaccata gcgatggcat cgaacaattc gataccctcg tgtgggatcg cgccaatccc
     3121 cccgcatcgg ttctaggcgg ggataagtac gtcatggtga cgaccaacta tatcgcgcaa
     3181 gtgatggccg attcgatgat accgctgtat gacgaaaacg ataagatcat caaaccgaac
     3241 gatgcggtcg tcttccgttc ggaccatacc gaggtcaaag aactggaatc gttcttcgcc
     3301 tatattcacg aacaggcttc cattcccgat cgctatgatc cctcctcgaa aaacgccacg
     3361 tcccgtttcg agggaatggc gttttgtccc tgattcgact caaccgttgc gatcttcggt
     3421 ttccatggac gcgttgcgtt agactggaat cgtaagcgga ccgtttccgc cgacaaccaa
     3481 cgaacctcaa ccaaaccgct tcggttttct ttggaccgtt gacgcaggga catcgtgaac
     3541 aactcggctc atggttcttt caacgaaatc gaaatatgaa tccgtcgtcc cgaacgcaag
     3601 gtgaacgtcg cgtagcccgg ttcgcttttt cgcggtggcg aaaaccccag cgcgtacggc
     3661 cccgcttggg aaggatcgtc cgaaggagta agctcgattt gctcgatcct cgcctccagc
     3721 aaaaacgttt cgctaccttc ttcggtggac atgcctcgca acgcaaagcg gcccgagcga
     3781 agtaaaatca cggttctttc caccagacgc gtcaaatgat tcggccgttg cgccgggtct
     3841 gtcggtttcg cactcacaat tttcccttcg ccatccacgt cgacctggac ttccaacgtt
     3901 cccgcatttc ccaacggaag cgtctcccat accgggtctg tggtattcgc aataggcatg
     3961 gctcgcgcga aggcggtcgc caggttgcgc acccgccgcg aacccgcagc gccgaaagag
     4021 gaggtccccg ccacctgccc accgtcggac cgccctgccc acaaaccatc ttcctgacca
     4081 ccgtcgattc ggtttgccaa cgcgttggca tccgcgccac cgtcgagttc aaaagattca
     4141 tctttcacag aagcagtggg tgcaggcaga gccgacgggt tcgatgaaat ggacggtgct
     4201 gcaagtgtgg aaatcggtga cggggatggc gcgggcttga ctacgatggg tggctcaggt
     4261 tctttgggta ggtttgcagt aggacgaggg gcaagcacgg tagcgggcga aggggggggc
     4321 accaccgctg gcgggttggt tgatacccta gaaacattag taaattcaac aggtttagag
     4381 atttcctcga cattgatgtc gatcgcgttg cccgtccatt catccggtga gaatacgtgc
     4441 tccacatctt cgatccccgt caccgttcga agtcccatcc caagcatgac cccgtgcaga
     4501 atcagggaag cgaaaacaca gaacaacgtg ggaacgtcaa ccttcatgct caacaagtca
     4561 ttcgttgcaa cgaaagtagc gtgatggtat cgaaccgtca ttggcttgtc ttgtgggtat
//
LOCUS       DAOSOR010000481         4574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_18955_length_4574_cov_3.512724, whole genome shotgun sequence.
ACCESSION   DAOSOR010000481 DAOSOR010000000
VERSION     DAOSOR010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18955_length_4574_cov_3.512724"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1934
                     /locus_tag="PK710_20075"
     CDS             192..1934
                     /locus_tag="PK710_20075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12085.1"
                     /translation="MRRSLSHGPLAASILIGALAGLLAGGVSCGSSEDDSAVPPKTTT
                     DGGTEATIQEEASVDVNADTPEEGFQIPNEFDDFPTAPIVGDGLPANIGALFDAGTAA
                     SSGGPCLSEPPIDAMVPHNWPALPFEWMASPEHNVFELTLSVDNQVHDLVVYTKQPTY
                     TMEASMWSNLMQHSRGHDVTITLRGAHFDGTSITAGPALGVSGNIHIAPVAAKGAVVY
                     WDASGGTSFEGFQAGDAKPVTVLTPSSAGNAPSGHETTCISCHASASKGKLLVYTADS
                     TDGSRVLDVRMMDGTGVPSPTMVSPSALSLLGRHKQSAPVTTLSEQSSLQSMVISVFI
                     EPTATSGHSEVIWTDLLTPDLQGWGIVARDGDSREASSPSWSHDGSTIAYVSSDVAGE
                     GVIADGEMDIYTVPFNKGAGGPAKPLEGASDPVMREFYPVYSPHDTWIAFNRTELAVD
                     SYDEPTAELFIVPGKGGVAQRLRANDPPACTGLVSPGLTNSWPRWAPQALEHEGKRYY
                     WLVFSSKRYAGSVASNGDWMAQLYIAAVVTQVTEQGETYLADYPPLYVTAQKASARNH
                     TPVWDYFEVPTIPK"
     gene            2048..2311
                     /locus_tag="PK710_20080"
     CDS             2048..2311
                     /locus_tag="PK710_20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12086.1"
                     /translation="MPLIEIEEAARRLARAIASDISLYNEDKITQGVKDDNLFEVLEE
                     EIEEGRALYNSRVSPKLYGRNLYERAIVDILVRSKGHVPSKLW"
     gene            2325..3317
                     /locus_tag="PK710_20085"
     CDS             2325..3317
                     /locus_tag="PK710_20085"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOT12087.1"
                     /translation="MSNPAGRFTFVVAPADGPLRLDIVVLEQLRRQGREVSRSVLHQW
                     FREGRVCIDGAPAKAATRSRCGATVEVDPAPPPASEAEPDPDLVIEIVYKDEHLLVID
                     KPAGLVVHPGRGHPGKTLVNGLLALDCFDPSVIDDDANPEAVLRPGIVHRLDKDTSGL
                     LVVARTRQAREGLKELFASHRIDREYEAIVVGRAVNATYDTFHGRDPSHRLKFTTRNP
                     VAPRRAITHVTLEQELAQGLASLVRCRLETGRTHQIRVHLFERGNTPVLGDALYGKGA
                     LQPWLRQVEQSLGRHWLHAGILGFEHPISGKAMRFTSPLPEELVMCLEALRQGG"
     gene            complement(3343..>4574)
                     /locus_tag="PK710_20090"
     CDS             complement(3343..>4574)
                     /locus_tag="PK710_20090"
                     /inference="COORDINATES: protein motif:HMM:NF013810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12088.1"
                     /translation="RPCAPIPSALRPYPVGLAHGRSGSPGQMKDIINALQFAGHCVFG
                     EEYGKINGKGQTGMAHLNQSGAEFAAVVDKVLKKTGAKKVNVVGYSEGGMVIDNYILA
                     KGGASKVNRMVGFAPGHHPYAHLGLSTIIDGIVFCPNTMEAVHNLLPPFTSNIKSQDI
                     VAAALGLASFVGQHLSPADQEIVESDFAADLFDANYWISLHGGLSEPPGHFLMVGQSE
                     RSLPTNDSAPEVCYTNLISPLDMMVGASAGFLDEGPNVSNIVLPSTTDHVTVIEDKVG
                     IATMLLAFETPCNPTPKPDPEGTEPQQDDDTSEAGVHADGGTNDESWPSEENSTDEND
                     DREDKASVADGGSPPDDHQDRDHNPSNSPSRHPMRDDSQAGCACASMNTTSTVPDLYP
                     LVVLLAMAGLRRRRAIQ"
BASE COUNT          942 a         1212 c         1409 g         1011 t
ORIGIN      
        1 cgataaccgt ggcgcggtat atccacgggt ttcgcaatct cggggggctc gtctcgtcca
       61 tcgtctccga ccatggcagg taggctagcg cgttcattgt catcgaggaa ccgtgatgcg
      121 cggtgttgcg ttggacatgg ggaaaggaac gtgacacagt tttacgggct tatgtggact
      181 tggaggaaat catgaggcgt agcttgagtc acggtccttt ggcggcttct atccttatcg
      241 gggccctggc cggattgctt gcaggcggcg tgtcctgcgg cagttcggaa gacgattccg
      301 cggtgccacc caaaaccacg accgatgggg gtacggaggc gacgatacaa gaagaggcca
      361 gcgtcgatgt aaacgcggat acccccgagg aagggttcca aatacctaat gaattcgatg
      421 acttcccaac tgccccgatc gtgggcgatg ggcttcccgc caacatcggt gccttattcg
      481 acgcgggaac cgcagcctcg tccggtggac cttgtttgtc ggaaccgccc attgacgcca
      541 tggtgccgca caactggccg gctttgccct tcgaatggat ggcaagcccc gaacacaacg
      601 ttttcgaatt gaccctatcg gtcgataacc aagtccatga tttggtggtt tatacgaaac
      661 aaccgaccta cacgatggaa gcgtcgatgt ggtccaacct catgcagcac tcccgtggtc
      721 atgatgtaac gattacgttg cgaggggctc atttcgacgg aacctcgatc acggcggggc
      781 cggctttggg cgtcagcggc aatatccaca tcgcacccgt ggccgccaaa ggagcggtcg
      841 tgtattggga tgcgtcggga ggaacgtctt tcgaagggtt tcaagctggt gatgccaaac
      901 cggtgaccgt gttgacacca tcttcagccg gcaatgctcc gtcgggccac gaaacgactt
      961 gtatttcctg tcatgcatca gcttcgaaag gaaagctgct cgtgtacacg gccgattcga
     1021 ccgatgggtc gcgcgtgctc gacgtgcgga tgatggatgg gacgggtgtt ccaagtccga
     1081 ccatggtcag tccgagtgct ttgtccttgc tgggccgtca caagcaatcg gcgccggtga
     1141 cgacgctttc cgaacagtcc tcgttgcaat ctatggtcat cagtgtgttc atcgaaccta
     1201 cagcaacgag cggacatagc gaggtcattt ggactgacct tcttacgccg gatctgcagg
     1261 ggtgggggat tgtggcgcgc gacggagatt cgcgagaagc ttcatcgcca tcgtggagtc
     1321 atgatggatc gacgatagcc tatgtatctt cggatgtggc gggagaaggg gtgatcgcgg
     1381 atggggagat ggatatttac acggtgcctt tcaacaaggg ggcgggagga ccggccaaac
     1441 cgctcgaagg cgcgagcgat ccggtgatgc gagagtttta tcccgtgtat tcgccacacg
     1501 atacgtggat agctttcaac cgaacggagc ttgccgtgga ttcttatgat gaaccgacgg
     1561 cggagctatt tatcgttccg ggcaaaggtg gggtagcgca gcggttgcga gccaacgatc
     1621 ctccggcttg tacgggattg gtgagccccg gtttgacgaa ttcatggccg aggtgggcac
     1681 cccaagcgct ggaacacgag ggtaaacgat attattggtt ggttttttcc tcgaagcgct
     1741 acgcgggcag cgtggcctcg aacggcgatt ggatggcgca gctatatatc gcggcggtcg
     1801 tcacacaagt gaccgaacaa ggagaaacct acctggcgga ctatccaccc ctttatgtaa
     1861 ccgcacaaaa agcctcggct cgtaatcaca cgccggtctg ggattatttc gaagtaccga
     1921 ccatacccaa atgatgattg acatccattc gtcgcgccac gcgcaaggac tgcccaccga
     1981 cggtatgcgt cgaacctttg acatcctccg cttgctcggg agatactgtt ttgcctagga
     2041 gagcccaatg cccttgatcg agatagagga agccgcccgt cggttggcac gtgcgatcgc
     2101 aagtgatatt tccctataca acgaggataa aatcacccag ggcgtcaagg acgacaacct
     2161 tttcgaggtg ctcgaagagg aaatagagga aggacgcgct ctttacaaca gccgcgtgtc
     2221 tcccaagctc tacgggcgaa acctttacga acgggcgatt gtggatatcc tcgttcgttc
     2281 caaagggcat gtcccatcca agctctggta atcgcgattc gttgatgtcc aaccctgccg
     2341 gtcgttttac ctttgtcgtc gctccggccg atggccccct gcgtctcgat atcgtggtgc
     2401 tcgaacagtt gcggcggcaa gggcgagagg tatctcgcag tgtcctgcat caatggttcc
     2461 gcgaaggacg ggtttgcatt gatggtgcgc cggccaaagc agccacgcgt tcgagatgcg
     2521 gcgcgaccgt cgaggtcgat ccggcgcctc cccctgcctc ggaggccgag ccggatcctg
     2581 atctcgtcat agaaatagtc tataaagacg agcacttact tgtgattgac aaacccgcgg
     2641 ggctcgtcgt tcacccagga cgcggacatc cgggcaaaac cctcgtcaat gggttgctag
     2701 cgctggattg tttcgatcca tccgtgatcg acgacgatgc gaatcccgag gcggtcttgc
     2761 gtcctggcat cgttcaccga ttggacaagg acacgagcgg attgctggtg gtggctcgta
     2821 cgcgacaggc cagggaaggg ttgaaagaac tgtttgcgag ccatcgaatc gatcgagagt
     2881 acgaagcgat cgtggtgggg cgcgcggtca atgcgacata cgataccttt catgggagag
     2941 acccgagtca tcgattgaag tttacgacgc gaaaccccgt ggcgcctcgt cgtgcaatta
     3001 cccatgtgac gctggagcaa gagcttgcgc aggggttggc tagccttgtg cggtgtcgtt
     3061 tggaaacggg acggacgcac cagatacggg ttcatctttt cgagagagga aacacaccgg
     3121 tgctgggcga tgcgctctac ggaaaagggg ccttgcaacc atggcttcgg caggtggagc
     3181 aatcgcttgg aaggcattgg cttcacgcgg gaattctcgg gttcgagcat cccatcagcg
     3241 gaaaggcgat gcggtttacc tcgccgcttc ccgaggaact cgtcatgtgt ttggaggcgt
     3301 tgcgacaggg ggggtgaagg atcggtgttt gaccatttcg agtcactgga tggctcgtcg
     3361 acgtcggaga cccgccatcg ccaacaggac gacaagcgga tacaagtccg gcacggtcga
     3421 tgtcgtattc atcgacgcac aggcacagcc cgcttgcgaa tcatcgcgca tcggatggcg
     3481 gctcggagag ttgctgggat tatgatctct atcctggtga tcgtctggtg gggagccacc
     3541 atcagcaacc gatgccttgt cttcgcgatc atcgttttcg tccgtggagt tctcctcgct
     3601 cggccatgat tcgtcgtttg tgccgccatc ggcgtgaact cctgcctcac tcgtgtcatc
     3661 gtcttgctgt ggttctgttc cttcagggtc cggtttcggt gttggattgc atggagtttc
     3721 gaacgccaac agcatggtgg caatgcctac tttgtcttcg atcaccgtta cgtggtcagt
     3781 ggtcgaaggc aacacgatgt tggatacgtt gggtccttca tcgagaaatc ctgcgcttgc
     3841 gcccaccatc atgtcgaggg ggctgatgag attggtgtaa cagacctcgg gagcgctgtc
     3901 gttggtgggc aggcttcgtt ccgattgacc aaccatcaag aagtgacctg gcggttcgga
     3961 aagccccccg tgcagagaga tccagtagtt ggcatcgaat aagtccgcag cgaagtcgga
     4021 ctcgacgatc tcctggtcgg cgggggacag gtgctggccc acaaaagagg ccagccccag
     4081 tgcggcggca acgatatctt gggacttgat gttcgacgtg aaagggggga gtaggttgtg
     4141 aaccgcttcc attgtattgg ggcaaaacac gattccatcg atgatggtgg acaacccaag
     4201 atgtgcgtag gggtggtggc cgggggcgaa gcccaccatc cgattgacct tgctcgcgcc
     4261 gcctttggca agaatgtagt tgtcgatgac catgccgcct tcggaataac cgaccacatt
     4321 gacttttttg gctcctgttt tcttgagcac tttgtcgaca acggcggcga actcagcgcc
     4381 agactggttc aagtgcgcca ttccggtttg cccttttcca ttgattttcc cgtattcctc
     4441 gccgaatacg caatggccgg cgaattgaag agcgttgatg atgtctttca tctgcccagg
     4501 agagccgctt cgcccatggg caaggccgac gggatagggg cgcaaggccg acgggatagg
     4561 ggcgcaaggc cgac
//
LOCUS       DAOSOR010000482         4555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_19061_length_4555_cov_4.134222, whole genome shotgun sequence.
ACCESSION   DAOSOR010000482 DAOSOR010000000
VERSION     DAOSOR010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19061_length_4555_cov_4.134222"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2254
                     /locus_tag="PK710_20095"
     CDS             <1..2254
                     /locus_tag="PK710_20095"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HOT12089.1"
                     /translation="TFYVLKGQSLEIQTSALLVNDTDPDGDVLSLRVIGPPTKGSLTP
                     TATGFIYAPSKEALGEDRFTYEVADKVGATDTTEATIHVANTIYYVSNHGDDSAPGTS
                     PEHPWKSPNVWATNRVKPGDVVLLERGSVFRETLSFDLSSSNTASRVLGAYGNGPDPI
                     VSGSEPVKEWTEVGKGVWRAPFPERPAHLYVNKTWQTPARTPNSGSYYWSGASPDPGS
                     GGHITTLNLPDLNQPKDAHVGATLRMRTRNWAFTHRRIVASSPGSVTVDTDQHFKGFE
                     AAVHEGGFAYYVDGKRDYLDAPGEWAYDDTEKMLYYFPLDANHKPSSELVEAVIREKG
                     IDISNGMGRDTTVYVDGLRVEQTKDVAVAFWSPSQGNAVLRRVDLRASHKGVTVWMDN
                     VRIIESTILDMIDEGGRFGEAGGDSGVGVGKSELLRSTVANIGLIPGYGTSGWGCQGI
                     VAKSPGFVARHNIFRDIGYTAMEVGTDALIEENLVIHALSSLNDGGSIAFSNTDGLSI
                     RNNIVVDAMGSFKDSVDENSNPFVIPYKRLAMGIYFGDKGVKNVLVEGNVVIGHSMGI
                     FIDHTGQSEGNTVRANLSYGNEDVQIANSDQSLFHGPQAGPCVPSYHDNLLDNWMIPS
                     TRGQWLYTEMNTKCSPPVNWGTIGNNHYALLYHSQVHVMNRDSLANEADRKNYSFEDW
                     KNTQEPTATRTPETVDLEDGESPSILYNAARGPRIVDLTGKWQNLEGEPITKPVVIGP
                     YSAVVLIPRH"
     gene            complement(2491..3591)
                     /locus_tag="PK710_20100"
     CDS             complement(2491..3591)
                     /locus_tag="PK710_20100"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOT12090.1"
                     /translation="MAEFHDDGDAVLVQLSQRQGDISLKTWGERRAWEQVTRILRNGK
                     VKIRSQQEYDLEFEVPRSEFDKVWESLKRLENIESSHEKQVDLSAQYSECSARLRFAR
                     HARETAARLPLDAAPTTAQEEIRRWDRRIEEFHKDLERIEEKSRIAIIRVHLLPQPRY
                     ESAGTALHLSMLGTGHKEMFGRQQTVMAVGLDARGMMGAPLGRSLGLDARAGASLDGG
                     IYVEGRVLMGLGAALGRRASLGLAPGIGTRGLSRWHIPFAVEVPLELFGYARVTDTVQ
                     WKGWARGAGVFGEEMIGSGSFGPRKRKAGTESVPFFDEFSAGTSLLFGWSQSDDPSRR
                     TGPYLAGAYHELMGQKVFEISMGVGMSLWDVR"
     gene            3857..>4555
                     /locus_tag="PK710_20105"
     CDS             3857..>4555
                     /locus_tag="PK710_20105"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HOT12091.1"
                     /translation="MWCKILPVAVGLLLVACESSSSDDGSRGGADSGTEASAGQGGNA
                     GQGGSGGQAGNAGQGGSSGQAGAAGLGGSAGQGTGGSAGEGAGGGGGAEPQGSCNEVD
                     PQAKPPVVFWPKPVRAYPGIPFTYTMVAYDEDGLELCYDVEGAPVGLSINGKGDISWA
                     PTEADTGKHEFTVHITTPQGGRKSVEVSLEVTKNGFVFAELGGHDGAAGTIDDPFGTI
                     EQGLAALKPSEAKTL"
BASE COUNT         1038 a         1245 c         1285 g          987 t
ORIGIN      
        1 gactttttac gtattgaaag gtcaatcgct cgagattcaa acgagcgcgt tgctcgtcaa
       61 tgacaccgac cccgacggcg acgtgttatc cctcagggtg attggaccac caacgaaagg
      121 aagcttgacg ccaaccgcta caggtttcat ctacgctcca tcgaaagaag cactcgggga
      181 agaccgtttt acgtatgagg ttgccgacaa ggtcggcgcc accgatacga ccgaggcgac
      241 gatccatgtg gcgaatacca tatactacgt aagcaaccac ggggatgatt cggcgcccgg
      301 cacgagtccg gagcatcctt ggaaaagccc gaatgtatgg gccaccaatc gcgtaaagcc
      361 gggtgatgtc gtgttgctcg agcggggatc ggtttttcgt gaaacccttt ctttcgactt
      421 atcgagcagc aataccgcct ctcgcgtgct tggcgcgtat ggcaatggac cggatccgat
      481 cgtatcggga agcgagccgg tcaaggaatg gaccgaggtg ggcaaaggtg tctggcgcgc
      541 cccgtttccc gaacgacctg ctcatcttta cgtcaacaag acctggcaaa ccccagctcg
      601 cacgcccaac tccggctcct attattggtc tggagcttct cctgatccgg gttcaggagg
      661 gcatattact actttaaacc tacccgactt gaaccaaccc aaggatgctc acgtgggagc
      721 aaccctgcgt atgagaacgc gcaactgggc gtttactcat cgtcgtatcg ttgcctcctc
      781 ccctggctcg gtgaccgtgg ataccgacca acacttcaaa ggtttcgagg cagccgtcca
      841 tgaaggcggg tttgcgtatt atgtggacgg caagcgtgat tacctcgatg cgcccgggga
      901 gtgggcctac gatgacacgg agaaaatgct ttactatttc cctttggatg ccaatcacaa
      961 gccatccagc gaattggtgg aagcggtcat tcgtgaaaaa ggcatcgata tctccaatgg
     1021 aatgggccga gatacaactg tttatgtgga cggcctgcgt gtggagcaaa ccaaggatgt
     1081 tgccgtcgcg ttttggtcac ccagtcaagg aaatgcagtg ctgcgtcggg tcgaccttcg
     1141 agcgagccac aagggggtta ctgtctggat ggataacgtg cgcatcatcg aatcgacgat
     1201 ccttgacatg atcgatgagg gtggccgttt tggggaagct ggcggcgatt caggggtcgg
     1261 tgttggaaag agcgaactat tgcggagtac ggtcgccaat atcgggttga tacccggtta
     1321 tgggacgagc ggttggggtt gccaggggat tgtggcgaaa agcccgggat ttgtcgcgcg
     1381 ccacaacata tttcgcgata tcggatatac cgccatggaa gtgggcaccg acgccttgat
     1441 cgaagagaac ctggtcattc acgcgctttc ctcactcaac gatgggggct cgatcgcgtt
     1501 ttccaatacg gatggcttat cgattcggaa caatattgtc gtagatgcga tgggaagctt
     1561 caaagacagt gtcgatgaaa acagcaatcc tttcgttatc ccttataagc gcttggcgat
     1621 ggggatctat tttggcgaca agggcgtcaa gaatgtgctt gtcgaaggaa acgtggtcat
     1681 tgggcattcc atgggaattt tcatcgatca caccggccag tcggaaggca ataccgttcg
     1741 cgccaaccta tcgtatggaa atgaagacgt tcaaatcgcc aactcggatc agtccttatt
     1801 tcacggtcct caagctggcc cctgcgttcc ttcttatcac gacaacctgc ttgataactg
     1861 gatgatcccg tcgactaggg ggcagtggct ctacaccgaa atgaacacga aatgcagtcc
     1921 gcctgtgaat tggggaacga tcggaaacaa tcactacgca ttgctttatc atagccaggt
     1981 ccacgtgatg aaccgcgatt cgctcgccaa cgaagcggat cgaaagaact attctttcga
     2041 ggattggaaa aacacgcagg agccgacggc tacgcgtacg cctgagacag tggatttgga
     2101 agatggggag agtccgtcga ttctctacaa tgccgcccga ggtcccagga tcgttgattt
     2161 gacaggaaag tggcaaaacc tcgaagggga gcccatcacc aaacccgtgg tcatcggccc
     2221 gtacagcgct gtggtgctga ttcctcgtca ttgattggct tggatgcttt gctgcctgcg
     2281 ccatcgtgga cccgcgaaaa ctcgaaaaaa ggatgagata ggaggaaaaa ccttctgcct
     2341 tcccgcgcag aatcagaaaa acggtggcaa aacctcgctt tcttgtctcc ccatggcgag
     2401 cgtccggaga agccaagata gccccacgat ctccagaaca ttctcaagta attgaattta
     2461 cgtgtgtttg gcaggaaaaa ttgacatcgt tcaccgaaca tcccacaagg acatcccaac
     2521 ccccatgctt atctcgaaaa ctttctgacc catcaactcg tgataggcac cagccaaata
     2581 cggccccgtt cgacggcttg gatcgtcgct ttgcgaccaa ccgaagagaa gcgatgttcc
     2641 agcggaaaat tcgtcgaaga aagggacaga ctccgttcct gccttgcgtt ttcttggacc
     2701 gaaggaaccg gaaccgatca tctcttcccc aaacacacca gcgccgcgcg cccatccttt
     2761 ccattgcaca gtatcggtca cccgcgcata gccaaataat tcgagcggaa cttcgaccgc
     2821 aaaaggaata tgccatcgtg acagtcctcg cgttccgatc ccaggggcca agcccagcga
     2881 cgctcttctt cccagcgccg cccccagacc cataagtacg cgaccttcaa cataaattcc
     2941 gccatccagg gaggctcccg cccgcgcatc caatcccagc gaacgcccga ggggtgcgcc
     3001 catcatcccg cgcgcgtcga gccccacggc catcaccgtt tgctgccgac caaacatttc
     3061 cttatgccct gttcccagca tcgaaaggtg gagggccgtg cctgctgatt catagcgagg
     3121 ctgagggaga agatgcaccc tgatgatggc gattcgactt ttttcttcga tccgctccag
     3181 gtccttgtgg aattcttcga ttcgtcgatc ccatcgacgg atttcctctt gcgcggttgt
     3241 aggcgcggca tcgaggggca aacgggccgc agtctctctg gcatgtcgag caaaccgaag
     3301 ccttgcggag cactcggaat actgagcaga cagatccact tgcttttcgt gactgctttc
     3361 gatgttttcc aagcgtttga ggctttccca taccttgtcg aactccgatc gcggcacttc
     3421 gaattcaaga tcgtattctt gctggctacg aatcttgact ttgccgttgc gcaggatccg
     3481 ggtcacttgc tcccatgccc ttctttctcc ccatgttttg agagaaatat ccccttgccg
     3541 ttgggataat tgcacgagca ccgcatcgcc gtcgtcatga aattccgcca tttcttgggc
     3601 tttcgcaacg cgtgacaggg tcagcggcaa gaaaaggaaa gcgggtaaca gtagaacaac
     3661 agtccctggc cggtatgact ctcgagtacc cattgccgga taatgatatt tcaatggttg
     3721 atgaccaaca aggtcgggtt ttgctgaaca tcgctccatc ctcctccgat ggtggaaatg
     3781 gctcatattt acgatttttg gcgtcaaccg tgacattggc tatccctctt ggcccccgtc
     3841 ggtctacaat gcgttcatgt ggtgcaaaat ccttccggtt gctgttgggc ttctcctcgt
     3901 cgcttgtgaa agctcgtcgt ctgacgacgg ttcgcggggc ggtgctgatt cgggcacgga
     3961 ggccagcgct gggcaaggcg gtaacgcagg tcagggcgga agcggcggac aggctggcaa
     4021 cgcgggtcag ggcggaagca gcggacaggc tggcgcggct gggctgggcg gtagcgccgg
     4081 acaaggcacg ggcggtagtg cgggagaggg ggccggtggt ggaggtggcg cggaaccaca
     4141 gggaagctgt aacgaggtcg atccacaggc aaagccgccg gtagtattct ggcccaagcc
     4201 ggtgcgggct tatccgggga tcccttttac ctataccatg gtggcctacg acgaagacgg
     4261 cttggagctt tgctacgatg tagagggcgc gcccgtgggg ctcagcatca acggcaaggg
     4321 cgatatatct tgggcgccca ccgaagcaga tactggcaag catgaattta cggtacatat
     4381 caccacgccc cagggcgggc gcaaaagtgt ggaggtcagc ctcgaggtca ccaaaaacgg
     4441 tttcgtattc gcagagcttg ggggacatga tggtgcggcc ggcaccatcg acgatccgtt
     4501 cggcacgatc gaacaggggc tagcggcact caagccctca gaggcaaaaa cgctg
//
LOCUS       DAOSOR010000483         4552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_19086_length_4552_cov_3.989104, whole genome shotgun sequence.
ACCESSION   DAOSOR010000483 DAOSOR010000000
VERSION     DAOSOR010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19086_length_4552_cov_3.989104"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2416
                     /locus_tag="PK710_20110"
     CDS             <1..2416
                     /locus_tag="PK710_20110"
                     /inference="COORDINATES: protein motif:HMM:NF025316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HOT12092.1"
                     /translation="HGPLAFGLRGPLGGGSEATSTAFLLISYLVGGCAEGGDEPSGGG
                     SGGKQNPTCSCQPGTTATCDCPGGGKGVQTCEIDCVSYTTCDCSDAGATGGSGGTGGG
                     STGEMGGSGGQNTGGSPTGGAGGEAASGGSAGSAGAAAGSAGSAGATGDVDVCPGEPL
                     HLSGSGSSIRQGSATGDTSTLTHDYLGNCGSALSKDAVYVVTPDVDGVLRADLGGSEA
                     TSFDSILYARTDCTDQDSEIVCDDTYVKGSEKLEFDVLKNTPYYLFVDGYNKEAGPYE
                     LNVQVIPKGCGNGIKEPGEDCDDGNQIDDDWCHNDCTINPNPAGDTCPGMTMALTGTG
                     TQDRVGTIAGDTSSFSSSYSSSKCVSSSTASAKDFVALVKPDVDGTMTCDLGGSTATP
                     WDSVLYVRSQCDNVSSELACNDSASTGAEKVTLPVVKNTPYYVFVDGYGAAKSGPFVL
                     TCTIKVAYCGNGTVEGTEECDDGNNTSGDGCSSSCKIEPKGPADICPGKTLQLKESNG
                     TYIAFETGSTTGQTHAYVGTCGGTSTAPDQVYKFNSGVGGRVTVSIPTEGTTFDTVLY
                     VRKDACSGPNATQVECKDTTGKGGESITFTAPPNTDYWVFVDGYSGANGNFSLEIKVV
                     PTCGDGTLDVGEECDDGNKTSGDGCSASCKIESTCGSLSEDEPNNTYTSPRLISAACG
                     TFRIHNAAISPAGDSDYFRINGLVEKTKVDAYAYVTTFGSCTSGAPLVISLWKNPIAT
                     PAADYGLCSGQTGNVACSASSTDGNCAKFSYTIPKDGDGDYLLKVHGSSSSSSVSSYG
                     LLVIVH"
     gene            2577..3275
                     /locus_tag="PK710_20115"
     CDS             2577..3275
                     /locus_tag="PK710_20115"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOT12093.1"
                     /translation="MSQTLACVILFDIDGTLLTGPHRCPSPGVAAMDASATRYTGHTG
                     LYERVEFAGRTDRQIARDLLVAGGNPEPTESQIVGLIECYLEMLEQGVQQRPYRALEG
                     AREAVEALRHTEAIVGLGTGNVAKGAQIKLESAGLVDLFDLSLGGYGDDAESRDELLR
                     VGARRCDPTERLPIVIVGDTPHDVRAALAIGARCLAVTTSYFDSHALRESGAHDVVGR
                     LDDRIVERIGRLVT"
     gene            3272..4300
                     /locus_tag="PK710_20120"
     CDS             3272..4300
                     /locus_tag="PK710_20120"
                     /inference="COORDINATES: protein motif:HMM:NF013479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HOT12094.1"
                     /translation="MSDKTESPTPRRLNKARTEGNIPFSSALLQASGMLIVVALAPSA
                     LSATAQQTTKLFMECLQHASDPLAVSSSAILWMPVVLTAPMLATVAVVVALVGYLQTN
                     GVIAFGKLAPDLTRLSPSSLWRSLLSPQRLFGIVRAMATALVVIALVIHRFESHMVDF
                     VRTAGRLHEAAVVAGTLTRGIVRDVLLVMLMLAAVDWVVAYRAWWSRLKMTHAEVKRE
                     VRETEGDPQIKAARERAHQEVLYSATIQRVQEATVVIVNPTRLAHALRYEEKEDDAPV
                     LIAKGEGALAKRMVEAAQAWGIAVVQNIPVARALSELTEGEAIPPGMYEAVAEILRDI
                     AEHHVSQP"
     gene            complement(4363..>4552)
                     /locus_tag="PK710_20125"
     CDS             complement(4363..>4552)
                     /locus_tag="PK710_20125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12095.1"
                     /translation="AFASGVFEAQRPHYALIEAIEALARAREANHPKAEEACLSFLRL
                     LYENQQHAGASMWLRSSI"
BASE COUNT          962 a         1174 c         1413 g         1003 t
ORIGIN      
        1 acacggtccc cttgcctttg gattgagggg tccgttgggg ggtggatctg aagcaacgag
       61 taccgctttt ttgcttatat cgtatcttgt cggtggatgt gcggaaggtg gagatgagcc
      121 ttcagggggc ggttcgggag gaaaacaaaa cccaacctgt tcttgtcagc ccggcacgac
      181 cgcgacctgt gattgtccag gcggtggcaa aggcgtgcag acttgcgaaa tagactgcgt
      241 ttcctacacg acctgcgatt gcagcgatgc gggtgcgacg ggcggaagtg gaggaacggg
      301 aggtggttcg accggtgaaa tgggaggaag cggcggacaa aataccggtg gctctcccac
      361 cggcggggct gggggtgagg ccgccagcgg gggaagcgct ggtagcgctg gggctgctgc
      421 gggcagcgcg ggcagcgcgg gtgcgacggg agatgtggac gtgtgtccgg gtgaacccct
      481 ccatctatcc ggatccggtt cttccattcg tcaaggctcc gccaccggcg acacctccac
      541 gttgacgcat gactacctgg gtaattgtgg ttctgccctt tccaaagacg cggtttatgt
      601 ggttacgccc gacgttgacg gagtccttcg tgcggacctc ggtggatccg aggccacgtc
      661 tttcgactcg atcttgtacg cgcgaacgga ctgtacggat caggattctg aaatcgtttg
      721 tgacgatacg tacgtgaaag gaagcgagaa actcgagttc gacgtgctca agaacacgcc
      781 ctattacctt ttcgtggatg gctacaacaa ggaagcgggg ccgtacgagt tgaacgttca
      841 agtcattcca aaaggttgtg gcaatgggat caaggaaccg ggggaagact gtgacgatgg
      901 caaccaaatc gacgatgatt ggtgtcataa cgattgcacc atcaacccca atccagccgg
      961 ggatacatgc cctggcatga cgatggcatt gacgggaacg gggacgcagg atcgtgtggg
     1021 aaccatcgcg ggggatacct cttcgttttc ctccagctac agctcttcca agtgcgtttc
     1081 atcaagcacg gcgtcagcga aggacttcgt tgcgctcgtc aagcccgatg tagacggtac
     1141 gatgacgtgt gatctgggcg ggtcaacggc aacaccttgg gattccgttt tgtatgttcg
     1201 ttcccaatgt gacaacgtct cttcggaact tgcttgcaac gacagcgcaa gcactggagc
     1261 tgagaaagta acgctcccgg tggtgaaaaa caccccctac tatgtgtttg tcgatgggta
     1321 tggagcggcc aaaagcggtc ctttcgttct gacttgcacc atcaaggtcg cctactgcgg
     1381 aaacggtacc gtcgagggga cagaagagtg cgatgacgga aataacacgt cgggcgatgg
     1441 ctgcagctcc agttgcaaga tcgaaccgaa aggccccgcg gacatttgcc ctgggaaaac
     1501 cttgcagctc aaggaatcga acggaacgta tatcgctttc gagaccggaa gcacgacagg
     1561 tcaaacccat gcttatgtcg gaacttgcgg tggtacatcc acggcacccg atcaggtgta
     1621 caagttcaat tcaggggtcg gaggcagggt gacggtatcg attccgacag agggaacgac
     1681 gttcgacacc gtgctttacg ttcggaaaga cgcttgttca ggccccaatg cgacccaagt
     1741 cgagtgtaaa gacaccacgg gaaaaggcgg tgaatccatc accttcaccg ctccaccgaa
     1801 tacggattac tgggttttcg tcgatgggta cagtggtgcg aatggcaatt tctcactgga
     1861 aatcaaggtg gttcccacgt gtggggatgg taccttggat gtgggggagg aatgtgacga
     1921 cggcaacaag acctcgggag atggttgcag cgccagttgc aaaatcgagt cgacttgcgg
     1981 ttcgctgtcg gaagacgaac ccaacaacac ctatacctct ccgcggctaa tctccgcggc
     2041 ttgcggtacg ttccgaattc acaacgctgc catctctccc gcgggtgatt cggactattt
     2101 ccggatcaat gggttggtcg aaaaaaccaa ggtggatgcc tatgcttacg tcacgacgtt
     2161 cggctcctgt acctcggggg ctcccctcgt catttccctg tggaagaacc ccatcgcgac
     2221 tcccgctgca gactatggcc tctgctctgg acagacggga aacgtcgctt gctccgcaag
     2281 ctccaccgac ggcaactgcg ccaagttctc ttacaccatt ccgaaagatg gagatgggga
     2341 ctacttgctg aaagtacatg gttcgagttc gagcagcagc gtgtcgagct atggtctgct
     2401 cgtcatcgtt cattgatgta ctgactgccg acagtttccc gcacccgtct ttttgatcct
     2461 ttccggctat ccaacgctca tcttcgagaa acctgatagg ctcgggcgat gcctggaatg
     2521 cctttcctca caatggtctc gtctcattcg gacccatgct cgatgggaga acggagatga
     2581 gccagacgct tgcctgtgtg atcttgtttg atatcgatgg tacgttattg acgggaccac
     2641 atcgttgtcc atctccgggt gtggcggcca tggatgcttc ggcaacgcgg tacaccggac
     2701 atacggggtt gtatgagcgc gtggagttcg cggggcgaac ggatcggcag atcgccagag
     2761 atttgttggt agcgggagga aatcccgagc ccacggagtc acagatcgtg gggctcatcg
     2821 aatgctatct cgagatgctc gaacaaggag tgcaacagcg cccttaccga gccctcgagg
     2881 gtgctcgaga agcggtggaa gccttgcgtc ataccgaggc aatcgttgga ttgggtacgg
     2941 gaaatgtggc caaaggagca cagatcaagc tcgaaagtgc gggtctcgtc gatctattcg
     3001 atctatccct tggcggatat ggcgatgatg ccgaatcacg cgacgaattg ctgcgtgtgg
     3061 gagcgcgtcg gtgcgatccc acggagcgat tgcccatcgt gatcgttggc gatacgccgc
     3121 atgacgttcg cgccgcgctt gccattggag cgcgatgcct ggcagtcacg accagttatt
     3181 ttgattcaca tgcgttgagg gaatcggggg cgcacgatgt cgtgggtcgt ctcgacgatc
     3241 ggattgtcga acgaatcgga cggttggtga catgagtgac aaaaccgaaa gtccgactcc
     3301 aaggaggctg aacaaggcca gaacggaagg gaatatcccg ttttcctcgg cacttctgca
     3361 agcttccggc atgttgatcg tggtggcatt ggccccatcg gcgctatccg caaccgctca
     3421 acaaaccacc aagttattca tggagtgctt gcaacacgca agcgatccgt tggcggtgtc
     3481 gtcatcggcg attctatgga tgccggtagt tctcacggca cccatgctcg cgaccgtggc
     3541 ggtggtggta gcgcttgtag gttatctgca aaccaatggc gtgatcgcgt ttggcaagct
     3601 tgcgccggac ctaacacggt tgtctccctc atcgctttgg cgctcccttc tttcgccaca
     3661 acgtctgttt gggatcgtcc gtgcgatggc gaccgcactc gtggtcatcg ccctcgtgat
     3721 tcatcgattc gagtcacata tggtcgattt tgtgagaacg gcagggcgtt tgcatgaagc
     3781 tgcggttgtg gccgggacgt tgacgcgggg cattgtgcgt gacgttttgt tggtcatgct
     3841 catgcttgcc gccgtggatt gggtggtcgc ctatcgggcc tggtggtcgc ggctcaagat
     3901 gacgcatgcg gaagtgaaac gcgaggttcg agagacggaa ggggatccac agatcaaagc
     3961 cgcgcgagag cgagcgcatc aagaggtttt gtattccgcg acgatccagc gggtgcaaga
     4021 ggcaaccgtg gtgatcgtga acccgacgcg tcttgcccat gcgcttcggt atgaggaaaa
     4081 ggaagatgat gctcctgtgc tgattgcgaa gggagaaggc gcgttggcga aacgaatggt
     4141 ggaggcggcg caggcatggg gcattgctgt ggtgcagaac attccggtgg cgcgtgccct
     4201 cagtgaactt acggagggcg aagccatccc acctgggatg tatgaggctg tggcagaaat
     4261 cctgcgggac atcgcggaac accatgtttc gcaaccgtga tgtacgcggt acgtttccct
     4321 ttcgtaaccc gttcatgctc caacgatctt tgcgaaccgt gtttagatcg aagatcggag
     4381 ccacatggat gcacccgcgt gctgttggtt ttcgtacagc agacggagaa atgacaagca
     4441 cgcttcttcg gctttcggat ggttggcttc tctcgcgcgc gccagcgctt cgatggcttc
     4501 aatcaatgca taatgaggtc gttgggcttc gaacactccg ctcgcaaatg cg
//
LOCUS       DAOSOR010000484         4540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_19159_length_4540_cov_4.071795, whole genome shotgun sequence.
ACCESSION   DAOSOR010000484 DAOSOR010000000
VERSION     DAOSOR010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19159_length_4540_cov_4.071795"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(386..538)
                     /locus_tag="PK710_20130"
     CDS             complement(386..538)
                     /locus_tag="PK710_20130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12096.1"
                     /translation="MTCPCLSVGMSRSLVMSGTARLTVGALRSFSNVGRQSSVLAMNS
                     ATARAQ"
     gene            complement(755..829)
                     /locus_tag="PK710_20135"
     tRNA            complement(755..829)
                     /locus_tag="PK710_20135"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(792..794),aa:Pro,seq:ggg)
     gene            complement(968..1564)
                     /locus_tag="PK710_20140"
     CDS             complement(968..1564)
                     /locus_tag="PK710_20140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12097.1"
                     /translation="MMERLFPFVCLVTALGCRPGAGDASTASSAIPSNSVVEGQSTAE
                     ASSLAKPVDSALAAIAYDLEPGCASDVDESKPAREQLDELGRLCAPGLSDFLTLAESP
                     ERVPIERSVDGCIRIGIVSSEKQVGATVVLSDSEGQVLESVTSRTPLLAPRDAVLCVR
                     AGRYEMRVSPLRAKAPLAVRVWEASAPGLGPAPTNSAK"
     gene            complement(1561..2463)
                     /locus_tag="PK710_20145"
     CDS             complement(1561..2463)
                     /locus_tag="PK710_20145"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT12098.1"
                     /translation="MHRVGFFILTTILASSTFGCVKKSEFDAKVAELAQCQGTLGRCA
                     QDFQNVQAQAKENEKKAVDTQALLDARTERANHDLAELQKNLDESTAFNASLRAELER
                     MGANVDKLLQEKGALSTSLEAAKARLEELRRAQAAAERRAQQMKQLIERFRKMADAGQ
                     LQVDIRKGRMVLQLPNDVLFDSGRAAVKPGGKLTLQEVASILRTIPNRQFQVAGHTDN
                     EPIRSSNYPSNWYLSASRAIKVVEILVEDGVAAHTLSACGYGEFDPLHPNDSEENKAK
                     NRRIEIVIQPNVDELLAVDSQTGW"
     gene            complement(2512..>4540)
                     /locus_tag="PK710_20150"
     CDS             complement(2512..>4540)
                     /locus_tag="PK710_20150"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOT12099.1"
                     /translation="YDSGPHPAAMHLCPATCNLVQSNANYRVDLSFACKANCGSSSVG
                     VDPVPLDMVVMLDRSGSMAWCANGKNPPCPGGALTRWNAVKQALGAYAGSTDAAGTTM
                     ALDYFPLPESDQCPVSHYMTPSVDWSLLPGKASDIQSSLNGTNPNGGTPTVPALQGAI
                     NLARQRAIANPNNKVVVVLATDGEPNNCSSTIAGVAAVAADGLAGGAIVTPAVTVGNE
                     ALETTTGTATGATGDEVTKGPFNIGFTYRYYGVNYTQFYVNTNGFVALGSSPPSNSYI
                     NTSLPDSGTPNGVIAPFWDDLVVVGNVYYQTLGTAPNRRLVVTWKNARHWSETGSCTF
                     EAVLHENGEISFRYGSMSGTYSDASSATIGIENASGTQGHTYSYNTARTLSNTSLRYK
                     WTEDTFPPIPTYVIGVGYESGLNSIAVSGGSSQAYIVSGGSASEFAAAMKAIRAQALG
                     CEYMIPSSSLGVPDPEAVTVRYSPGNGGNPVDFPLRSSTTCGSEHGYYFDNPANPQRL
                     YLCSASCDLLATDSKASVAIFYDCLGNYNNGVFTRDYNAAGMCPPGSSHLWSKWIWES
                     TTPNGTSIDFTVATADTQAGLDTAEEVPLLFSTPEGPSSLVGQAIGAKEGPPDTQSGS
                     ADVDQTLALKLQPRKKPFLRVRAHLRGAPPTFTSTPLLSHWNLTVSCENSE"
BASE COUNT          938 a         1327 c         1327 g          948 t
ORIGIN      
        1 ttggtccagc aagcgcaggt gcgcgggtgt ggggtggatc tcgtctggat cgtagttcga
       61 catactctta gaacgatcca gcggcgtgaa aatcaatctg ctgcctaact ttaatgcttt
      121 gatttcgcgt ccctgcggtg caggtctccc catggacggt acgagcggca tcgacggcac
      181 gatcgctctc tggcgaagaa ccgaccggaa aaattggtac ctccaggtct acgacccccg
      241 taccccgcgc gagtaccgag actcggcgca cacaaccgac gaagtcgtcg ctcgccagcg
      301 cctggctaac gctgaaaggc tcgtgattct cggggccttc gtgcctccgg ctgagcacca
      361 ccaagtcccg gcgtggctcg atctgtcact gcgcacgggc ggtcgcggag ttcatcgcca
      421 gcacgctgct ctggcgaccc acgttcgaaa aggagcgcaa ggcgccgaca gtcaaacgtg
      481 cggtgccact catgacgagg cttcgagaca tgcccacgga caagcacggg catgtcatgg
      541 gtgtgcatga tcgttgcaag gcgctcgacc ggacgatgga gcttgccaag atccgaggat
      601 atgttcggtt tcatgacctg cgtcccacgg cgtccacgag gctgaacgac gaagcgctcc
      661 gcaaccgtct ccggtttgta cccgcccccc atttgcccct tgattcaccg gaccacaaaa
      721 ccaaaaaccc gctgaatctt cagcgggttt ccagtcgggg cgagtggatt cgaaccaccg
      781 actccttgac ccccagtcaa gtgcgctaac caggctgcgc tacgccccgg ggttacggcc
      841 aaaccgaccg cagtccaaaa ggaagcggca gtgtatcgaa acacgcgcga actgcaacct
      901 caaaattcaa tcgattcatc ttcgaagatt cacgctccct tgcgacggaa aacatccacc
      961 acaaccctta tttcgcggaa tttgtgggcg caggcccaag gccgggcgcg gaagcctccc
     1021 acacccgcac cgcaagcgga gcctttgccc gaagcggcga cacacgcatc tcatagcgcc
     1081 ccgcgcgcac acaaagcacg gcgtcgcgcg gtgccaacaa gggcgtccgc gaggtgaccg
     1141 actccagcac ttgcccttcg gaatccgaaa gcacgaccgt cgcccccacc tgcttttcgg
     1201 aagacactat cccgattcgt atacacccat ccacgcttcg ctcgatcggc actcgctcgg
     1261 gggattcggc aagcgtgaga aaatcggaaa gccccggcgc gcaaagtcgt cccaattcat
     1321 cgagctgctc tcgagccggc ttgctctcgt ccacatccga cgcgcatccc ggttccaggt
     1381 cgtaggcgat cgccgcaagc gccgaatcca ccggtttggc caagctcgac gcttccgccg
     1441 tcgactggcc ttccaccacg gagttcgagg ggattgccga ggacgccgtc gatgcatccc
     1501 ccgcgcccgg ccggcacccc agcgccgtca caagacaaac gaaaggaaaa agcctttcca
     1561 tcaccagccg gtctggctat cgaccgccag cagttcatcg acattgggtt gaatgacgat
     1621 ttcgatgcgg cgatttttcg ctttattttc ctcggaatcg ttgggatgca ggggatcgaa
     1681 ttccccgtat ccacatgcgg atagtgtgtg agccgctacg ccgtcctcca cgagaatctc
     1741 gacgactttg atggctcggg aggcggacag gtaccagttg gagggatagt tggaggaacg
     1801 aatgggttcg ttatcggtat gcccggcgac ctggaattgg cgattgggga tggtgcgaag
     1861 aatcgacgcg acttcttgca aggtgagctt gccccccggc ttgacggcgg cgcgtccgga
     1921 atcgaagagc acgtcgttgg gcagttgcag caccatacga ccttttcgaa tgtccacttg
     1981 aagctgcccg gcgtcggcca tttttcggaa tcgttcgatg agttgtttca tttgctgggc
     2041 gcgacgttcg gcggcggctt gggcgcggcg cagttcctcg agacgcgcct tggcggcttc
     2101 caggctggtg gaaagggcgc ctttttcttg caggagtttg tcgacgttgg cgcccattcg
     2161 ctcgagttcg gcgcgcaaac tcgcgttgaa cgcggttgat tcatccaggt ttttttgtaa
     2221 ttcggcgagg tcgtggttgg cccgttcggt gcgggcgtcg aggagcgcct gcgtatcgac
     2281 cgcttttttc tcattttcct tcgcttgagc ctgaacattt tgaaaatctt gggcgcatcg
     2341 tcccagcgtg ccttggcatt gggccaattc cgcgactttg gcgtcgaatt cggatttttt
     2401 gacgcagccg aacgtgctgg aagcgaggat ggtggtgagg atgaagaagc caacccgatg
     2461 catgggcagg gtctagcatc agggttggga aaggaacaag ttggcggggg attattcgct
     2521 attctcgcag gaaaccgtaa gattccaatg agatagcagc ggggtgctcg tgaaggtggg
     2581 tggcgcgcct ctcaagtgcg cgcggacgcg gaggaacggt tttttccttg gctggagctt
     2641 cagagcaagg gtttggtcca catccgccga gcccgattgg gtgtctgggg gaccttcctt
     2701 ggctccaatg gcctgtccca cgagcgagga ggggccctcc ggtgtggaga acaacaacgg
     2761 gacctcctcg gcggtgtcga gcccggcctg cgtatcggcc gtggccacag taaagtcgat
     2821 gctggttccg ttgggggtag tggattccca aatccatttg gaccaaaggt ggctggatcc
     2881 gggggggcac attcccgcag cattgtaatc tcgcgtaaaa acgccgttgt tgtagtttcc
     2941 gaggcaatcg tagaaaatcg ccacggaagc tttggaatcc gtggccaaca aatcacaact
     3001 tgctgaacaa agatagaggc gctgcgggtt cgcggggtta tcgaagtagt acccatgctc
     3061 agaaccgcaa gtggtgctcg aacgcaacgg gaaatcgacc ggattaccgc cgtttccggg
     3121 tgaatagcga acagtgacgg cttccggatc gggcacgccc agcgaactgc taggaatcat
     3181 atattcgcag cccaaggcct gtgctcgaat cgctttcatc gcggccgcaa actcgctggc
     3241 gctcccaccg ctgacgatat acgcctgcga actcccgcca cttaccgcaa tcgaattcaa
     3301 cccggactca tatccaacgc caatgacgta ggtcgggatg ggaggaaatg tgtcttctgt
     3361 ccacttataa cgaagggacg tattgctcaa cgtcctcgca gtattgtaag agtacgtgtg
     3421 cccttgcgta cctgacgcat tttcgatgcc aatcgtcgcg gatgaagcgt cggaatacgt
     3481 ccccgacata gacccatatc ggaacgatat ctccccattt tcgtgtaata ccgcctcgaa
     3541 cgtacacgat cctgtttccg accaatgtcg agcgtttttc cacgtgacca cgagccgtcg
     3601 attgggtgcg gtacccaacg tttgataata tacgtttcca accacaacca agtcatccca
     3661 gaagggcgca atgacgccgt tgggagtgcc agagtccgga agactcgtgt tgatatagct
     3721 gttactaggt ggagagctac ccagcgccac aaacccgttg gtattgacat aaaactgcgt
     3781 gtaattgacc ccatagtacc gataggtaaa cccgatattg aaaggacctt tggttacctc
     3841 gtctccagtc gcaccggttg cggttccggt cgtcgtctcg agagcttcat tacccactgt
     3901 gaccgcaggt gtcacgatgg cccctcccgc cagcccatcc gctgccaccg ccgccacccc
     3961 cgcaatcgtc gagctgcagt tattgggttc cccgtcggtc gccaacacga cgacgacttt
     4021 gttattcggg tttgcgatgg cacgttgtcg agcgagattg atggcccctt gaagcgctgg
     4081 cacggttggc gttccgccat tcgggttggt gccgttaaga gaggattgga tatcggaggc
     4141 cttaccgggg agaagactcc aatcgacgga aggggtcata tagtgcgata cagggcattg
     4201 atcgctttcc ggaaggggga aataatcgag cgccatcgtg gttcccgccg catcggtgga
     4261 tcccgcataa gctccgagcg cttgtttgac cgcattccat cgtgtcaggg caccgcccgg
     4321 acacggcgga ttttttccgt tggcacacca ggccatcgag ccggagcggt ccaacatgac
     4381 caccatgtcc agtggaacag ggtcgacacc cacggaggag gaaccgcaat tggctttgca
     4441 ggcaaagctg aggtctactc ggtaattggc attgctttgc acgaggttgc aggttgccgg
     4501 acacaaatgc atcgctgccg ggtgcggacc cgaatcataa
//
LOCUS       DAOSOR010000485         4537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_19179_length_4537_cov_3.931727, whole genome shotgun sequence.
ACCESSION   DAOSOR010000485 DAOSOR010000000
VERSION     DAOSOR010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19179_length_4537_cov_3.931727"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..2224
                     /locus_tag="PK710_20155"
     CDS             191..2224
                     /locus_tag="PK710_20155"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOT12100.1"
                     /translation="MQPSTRPRPWRESRPELLIHNTVTDRLFRNERLLLLWAAIIVGA
                     CGGGFALGVRLLIDMNWWAFSYLRSLDRPLFVLLVLAIPALGGLVLGLIARISMAVGE
                     PGVEELRKSFLTTVGILAVPTALLRLLACAVTMTMGGSVGAEGPVARASTALGSWFAR
                     RFGLDDSRAQLLIACGVSASIAGIFHTPFAAVVFAAEVVLGVYAIRRLAYLLLAAGVS
                     LGLSRWGAGLDAPFSAPPFVLQSPLDLVIHAVVGTGCGALAVLLLKATEWLVSMRDRC
                     AGVWPSVLGGLMVGGVVLMVPQAVGVGYPALSGLLSNSVTISFVVAVLVAKLTATALT
                     LGSRGVGGVFSPSLVVGGGLGWLVGRAVHGVAPQTFAPASSYAAVGMVSMASAVVHAP
                     LTAAAFIVEITRSYDAVVPGVVAGVAASIMSATIRDEPVSGHRPGLPAQGPVQSSIEL
                     TPTTIEELIDPLRDTVPASLSAFELVNRLPAVRQSVLVVADGASVMGVISTRAAMRKD
                     GEALEGKTARDLMIDTGVLHKGDSVAQAIVQFMGHDAPALPVVSEQSQLVGLVWRSDL
                     MDFCAHDLLQNDLYFSDWAPMSGGDPSTRPLRHDVVALPVPPAFVGKTLREVDLGKRF
                     GVVCVGIRRATNTGAFVAVHVRPNLELRADDVLILTGNISDLDQIGLLTQQDG"
     gene            2339..2698
                     /locus_tag="PK710_20160"
     CDS             2339..2698
                     /locus_tag="PK710_20160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12101.1"
                     /translation="MDEDTRRRVCRLIAGIVVVDDELDESEDLFIDRMLAQFELSTEE
                     RDALFPIMDTKEAADEFRALGADVQKEALELLVQAAAVDRKYADEEKVYLHAVCEAAG
                     VSTVEVDRRVHDLIAGS"
     gene            2738..3010
                     /locus_tag="PK710_20165"
     CDS             2738..3010
                     /locus_tag="PK710_20165"
                     /inference="COORDINATES: protein motif:HMM:NF039355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HOT12102.1"
                     /translation="MLSPTRWFGARGRDRERASSSAALSLPATLTPRWKMTVKLVVFV
                     ALGALAGFAYHKLVGCRTGACPITANPWITTVYGAVMGFLLSGGIR"
     gene            3206..>4537
                     /locus_tag="PK710_20170"
     CDS             3206..>4537
                     /locus_tag="PK710_20170"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOT12103.1"
                     /translation="MLLSRCVGRVRWPVIALSIMACSGGACPLQLCSQPSQQNAVIAG
                     RPVFVLQTGHSDWVTDLAFNPSSRLIASSSVDQTVKLWDATSGALRATLHGHKGPVNA
                     VAWHPAGLFLASASADETLRVWDVRTAEELKTLYGHKGAIVSVDVSPDGASMASAGED
                     GTIRLWDAKTYAVRWTIEAGKERIAKVLFSRDSASIIATVGKSIKRWDAISGEFQREY
                     RGHEDWVEALAFSPDGRMLLSGGADKAIHLWNYAEAKIIRSFLGHTWPVWSVAFSPDG
                     LRIASGSGDKTIRIWRVIDGHLVRTLNRHTDHVKTVSFSSEGNLLASSGFDRSILLWD
                     TNVWDLRTTLPGQVRYLSSVQFQPQGELLATAGQKNTVTLWNFKSGQIQASLASDAFA
                     TWAVAFDPSGKYLATAGDEGVVKVWDVATRSVLRTLSVSEEPVRCVGWSPDG"
BASE COUNT          805 a         1091 c         1485 g         1156 t
ORIGIN      
        1 acgtgaaaag gccatgttcg cgagcgccga attggtgctg cgacgtcatg agcgggtcga
       61 acaactacgg ggggtcagca tcgagttgca agatcccctg cgggttcgtt ggtatgattc
      121 ttttcgtacc cgaatggatg agccatcggc gcggttcaaa gtcaagtagc gccgcgtggg
      181 gggcaatggc atgcaacctt cgacgcgccc tcggccttgg agagagtctc gccccgaatt
      241 gttgattcac aataccgtta cggaccgtct cttccgcaac gaacgcttgt tgttgttgtg
      301 ggctgccatc atcgtggggg cctgtggtgg tgggtttgct ttgggggtcc ggctgctcat
      361 cgacatgaat tggtgggcgt tttcgtatct tcgttccttg gatcgccctc tattcgtgct
      421 tctggtgttg gcaattccgg ccctgggcgg gctcgtactc gggcttattg ctcgtatctc
      481 gatggcggtt ggcgaaccgg gagtcgagga attgcgcaag tcattcctga cgacggtggg
      541 gattcttgcc gttcccactg cattgcttcg tttgctggct tgtgcagtga ccatgacgat
      601 gggagggagc gtaggagcgg agggaccggt ggcgcgagcc tccaccgcgc ttggttcatg
      661 gtttgcgcgt cggtttgggc tcgatgatag tcgagcacaa ctgctcatag cttgcggtgt
      721 ttccgcgagt atcgcgggaa ttttccatac accttttgcg gctgtcgttt ttgccgcgga
      781 ggtggtgttg ggggtgtacg cgattcggcg ccttgcgtat ttgttgcttg ccgcgggggt
      841 ttcgttgggg ttgtcacgat ggggcgcagg actcgatgcg ccattttccg cgccaccgtt
      901 cgtgttgcaa agtccgctcg accttgtgat tcatgctgtc gtggggacag gatgcggcgc
      961 attggccgtt ctgctcctca aagcaacgga atggttggta tcgatgaggg atcgatgcgc
     1021 tggggtgtgg ccgtcggttt tgggcgggtt gatggtgggt ggcgtcgttc tgatggttcc
     1081 tcaggccgtg ggggtgggct atcccgcgct gtcgggtcta ttgtccaatt ctgtaacgat
     1141 ttcgttcgtc gtcgcggtat tggtggcaaa actcactgcg acggcactta ccttgggaag
     1201 tcggggtgta ggtggggtgt tttctccatc gcttgtcgtg ggggggggcc tcggttggct
     1261 ggtgggccgt gccgttcacg gtgtcgctcc tcagacgttt gctccagcct catcgtatgc
     1321 ggcggttggg atggtctcga tggcgtcggc ggtggtgcat gctcccctca ccgccgcggc
     1381 atttatcgtt gaaattacac gtagttacga cgctgtcgtt cctggggtgg tggcaggggt
     1441 ggccgcctcg atcatgtcgg cgacgattcg tgatgagccg gtttccggcc atcgtccagg
     1501 gcttccagcg caaggtccgg tgcaatcgtc gatcgagttg acgccaacga cgatcgagga
     1561 gttgatcgat ccgttgcgag acacggtgcc ggcgtcgttg tctgctttcg agctggtgaa
     1621 ccgcttgcct gcggttcgtc agtctgtgct cgtggtggcc gatggggcta gcgtgatggg
     1681 ggtcatttcc acgcgtgccg cgatgcgaaa agatggggaa gcgttggaag gaaaaacggc
     1741 gcgggacctg atgatcgaca cgggggtttt gcacaagggc gattcggtgg cgcaagcgat
     1801 tgtgcagttt atgggtcacg acgcgccggc gctgcccgtg gtgtccgagc aatcacaatt
     1861 ggtcggtttg gtttggcgtt cggatctcat ggatttttgt gcgcatgacc ttttgcagaa
     1921 cgatttgtac ttcagcgatt gggcaccgat gtccggaggg gatccctcga caagaccttt
     1981 gcgacatgac gtagtagccc ttcccgtacc tccagcattc gtcggcaaga ccttgcgaga
     2041 ggtggatttg ggaaaacggt tcggtgtggt atgtgttgga attcgtcgcg caacgaatac
     2101 gggtgccttt gtggccgttc acgtccggcc caacctcgaa ttacgcgccg atgacgtgct
     2161 catcctaacg ggaaatattt cagaccttga tcagattggt cttctgacgc aacaggatgg
     2221 atgaagacgt gaccatcctt tccgaagtac actagggcct tgggtgactg cccattcgtt
     2281 tcgatatctg gcctttcggg gctatggatc ccttcgcttg tgtgatcatg gaggcatcat
     2341 ggacgaggat acccgccgca gggtttgtcg tttgattgca ggaatcgtgg tggtggacga
     2401 cgaactcgat gaaagtgaag atttgttcat cgatcgtatg cttgctcaat ttgagctttc
     2461 gacagaggag cgcgacgctt tatttccgat catggatacc aaagaggccg cggacgaatt
     2521 ccgggcattg ggagccgacg tgcagaagga agccctggag ctgttggtgc aagcagcagc
     2581 cgtggaccgc aaatacgctg acgaagaaaa ggtctatttg cacgcggtgt gtgaggcagc
     2641 gggagtatcg acggtcgagg tcgatcgacg cgtgcatgat ctcattgcgg gatcatgatt
     2701 atgaatccaa cgagctgtta ccgactctaa ccgcattatg ttgtctccca cccggtggtt
     2761 cggtgcgcgc ggtcgggacc gagaacgagc gtcgtcttcg gcggccctat cgttgcctgc
     2821 caccctgact cctcgttgga aaatgaccgt caagcttgtt gttttcgttg cgttgggtgc
     2881 ccttgcgggc ttcgcctacc acaaactcgt tggctgtcgg acgggggcat gccccattac
     2941 ggcaaaccca tggataacga ctgtctatgg tgcggtaatg ggttttttgt tgtccggagg
     3001 gattcgttga gccatgaaag gcgatgatga ccgacacgtg gcggggtgtg ttctggtgac
     3061 tcattggtga cggtcaagct tgccgttcct gtagtcctgc gttgacgaag cagagacgaa
     3121 cgggtgattt cgattgcctt gggtgtctgc ggagcttgct tctgtacgtg tgcgaaggat
     3181 aatcgagcat ggaaagactc gtttcatgct cttgtctcgt tgcgttggtc gcgttcgctg
     3241 gccggtgatt gcgttgtcca tcatggcctg ttcgggggga gcttgcccgt tgcagttgtg
     3301 ttcacagcct tcgcaacaaa atgctgtgat agccggtaga ccggtgtttg tcctacaaac
     3361 tgggcacagc gattgggtga cggatctcgc gttcaatcca agcagccgac tcattgcctc
     3421 ctcgagtgtc gatcaaacgg tcaaactttg ggatgcgacg agtggcgcgt tacgagccac
     3481 gcttcatggc cacaaagggc ctgtgaatgc agtggcctgg catcctgcag gactgtttct
     3541 agcgtctgcg agcgctgatg aaacgctgcg cgtttgggac gtgcgcacgg cggaggaact
     3601 caagacccta tacggacata aaggggcgat tgtatcggtg gatgtgagcc cggatggggc
     3661 aagcatggca tcagcagggg aagacgggac aattcgactt tgggatgcaa aaacctatgc
     3721 ggttcgttgg acgattgaag ccggaaaaga acgaatcgcg aaggtgttgt tctctcgtga
     3781 ttccgcttcg atcatcgcaa cggtgggcaa gagcatcaag cgttgggatg cgatcagcgg
     3841 agaatttcag cgtgaatatc gtggtcacga ggattgggtc gaggcattgg cgttcagtcc
     3901 cgatggtcgt atgttgctct ccggtggtgc ggacaaggcg attcatttgt ggaactacgc
     3961 ggaagcgaag atcatccggt cttttttggg acatacttgg cccgtatggt ctgtggcgtt
     4021 tagtccagac ggcttgagga ttgcctcagg cagtggtgac aagactatcc ggatatggcg
     4081 tgtcatcgac ggacatctcg ttcgaacgtt gaatcgacat accgatcacg tcaaaaccgt
     4141 gagcttttct tccgagggta atctgcttgc atcgtcggga ttcgaccgtt cgattctgct
     4201 gtgggatacg aacgtgtggg atcttcgaac gacgctacct ggtcaagttc ggtacctttc
     4261 gtcggtgcag ttccaacccc agggcgaatt gttggcgacg gctggccaaa aaaacaccgt
     4321 aacgctttgg aacttcaagt ccgggcaaat ccaagcgtct ttagcatcgg atgcctttgc
     4381 aacttgggcg gtcgccttcg atccctcggg aaaatatttg gcgaccgcgg gggatgaggg
     4441 ggttgtcaag gtatgggatg tggcaacccg ttctgttttg cgaacccttt ccgtgtcgga
     4501 agagcccgtt cgatgtgtcg gctggtctcc ggatggg
//
LOCUS       DAOSOR010000486         4537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_19180_length_4537_cov_3.855422, whole genome shotgun sequence.
ACCESSION   DAOSOR010000486 DAOSOR010000000
VERSION     DAOSOR010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19180_length_4537_cov_3.855422"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="PK710_20175"
     CDS             <1..1034
                     /locus_tag="PK710_20175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOT12104.1"
                     /translation="AHGKSSGLAASQTKPAKVVEARTSPRPTEKSLEPPQKPSKPTKA
                     SKALPSAATAAGRPDSAPPKTPAAPVRRSPLMRPPLPAIPVEPPRPSLEDRAERVKTR
                     LEQQPIEVRREYEEHLNMSWIHHDSALEGVVYSFQELQLAIDPTVTVVPDTSIQPICD
                     EIRRHKAAIDFILEMADAKKRQPVTTDLIKRIFLYLHPEEGDLKTLKYRKDVPQHRLY
                     FHEYAPPDKIAYAVRQVVEWINDPETRRTRTTLRIAARAHYDLLRIFPFSQDSGKVSR
                     LLMNFLLLRAGYPPAIIHSTERQRYYEALKGAPLSITHMVEEALENSIASIEKYLDER
                     TTRIRSFVS"
     gene            1148..1372
                     /gene="rpmB"
                     /locus_tag="PK710_20180"
     CDS             1148..1372
                     /gene="rpmB"
                     /locus_tag="PK710_20180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOT12105.1"
                     /translation="MARSQITGKRRMLANNVSHSNIKTKRWQLVNVQRRRIWVPELNR
                     FVRLDLTTRDLRTIDRIGLVAFAKKHGYKL"
     gene            complement(1406..1843)
                     /locus_tag="PK710_20185"
     CDS             complement(1406..1843)
                     /locus_tag="PK710_20185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12106.1"
                     /translation="MRQPNLLGDVPELTCLMMMASDPRGVLAVMHCAFDPQGPEVAPH
                     QVTVSRLAVPVRRGIPRVRPGSPIRLPIPIALLMEGRTPWAAVGIHKVEAVDWQIVMD
                     RVKADLSLEQSLRAALVDNHPERRVFAIVRATGSGAPRASEIK"
     gene            complement(1818..2423)
                     /locus_tag="PK710_20190"
     CDS             complement(1818..2423)
                     /locus_tag="PK710_20190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12107.1"
                     /translation="MTSRAAIALGSEPSMTESARMVLAKGGTSVDACITGFFTAAGAH
                     PGVLLGCVSLLVAGTGVGEHLFDGSVVQPGVGAPRPRGFQQDDLIPVSARIPVAATGA
                     MLSAVHAYGGSIPMSELATPGVRLAQAARAAGRAHLLRRVGDAGPVALRESSFVRALL
                     EIGGRHEGGNVTQQDLADVQASILEPCRMQGVIHASAEPFG"
     gene            complement(2420..3133)
                     /locus_tag="PK710_20195"
     CDS             complement(2420..3133)
                     /locus_tag="PK710_20195"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOT12108.1"
                     /translation="MQRVYIGTRTLQGALERYAKKFNLLEVRTEPEVPLASRTLRRWR
                     KSVPPSFVFSVVLPNVVTELRPSAAADRALQQTLEIATILESPVMLISHPVSVTPTAA
                     NRKRLEELVQRIPHDVVRLAWEPPGLWEEEEARNVASRLGLVLVRDAAREPLAPGSVA
                     YTRLRGLGDSRRLSQTRIDKTIERLQAFREAFVIVETDSPLAVAKAIHASEGRGAEPR
                     ISRTRHVEYALHAEDEEQE"
     gene            complement(3231..>4537)
                     /locus_tag="PK710_20200"
     CDS             complement(3231..>4537)
                     /locus_tag="PK710_20200"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HOT12109.1"
                     /translation="GYGGTGGYGGGGYGGSGGTGGTGGAGGIGGAGGTGATGGYGGTG
                     GTGPVCEGGPHDDCDGDGWTVAEGDCCDKPGFCSTTPALVNPGAFEYIGNGVDDDCDG
                     LIDNIQPPCDGMLNNTNSSDPMDYAKAIGLCQQTKETDPPPFRRWGVISGEFSLANGT
                     GVPASQSRAIRSGFGNLITSLNGGRLAILSTGHAASVNQANPNYAPFQPGQDFGKKSP
                     FPQDWYAANGYRLPNAPGCPEPSGDHANDPIMLRLRIRVPTNARSFSFASWFFSAEFP
                     EYVCSAFNDFFVVLIDSPQPEWPNPADKNLAVYRVTSPPKVFPIGVNLANDPQAQNLF
                     SVCKAGPIGCSGSTSGFAPCLSGPTFLQGTGFDDTSLGGCGRSNMVGGGTGWLTTVGN
                     VAPGQIMEIRIALWDTSDGVWDSLVLLDNWVWNVYPASPGTF"
BASE COUNT         1039 a         1421 c         1192 g          885 t
ORIGIN      
        1 cggcccacgg caagtcttcc ggtctcgcag cttcccaaac caagcccgcg aaagtcgtcg
       61 aagcacggac ttctcctcgg cccaccgaaa aatcgctcga accgcctcag aagccgtcga
      121 agccaaccaa agcctccaaa gcgctcccct cggcagctac tgctgctgga cgcccggact
      181 cagcgcctcc caaaacaccg gcagctcccg ttcgtcgctc accgttgatg cggccacccc
      241 ttccggcaat tccagtcgaa ccgcctcggc cttcgctcga agatcgcgcc gaacgtgtca
      301 aaacccgatt ggagcaacaa cccatcgaag tgcgacgaga atacgaagag catttgaata
      361 tgtcatggat tcaccatgac agcgcacttg aaggagtcgt ttactccttc caagaattgc
      421 agctcgccat cgatcccacc gtcacggtcg tccctgatac gagcattcaa cccatctgcg
      481 acgaaattcg tcgtcacaaa gccgcgatcg atttcatctt ggaaatggct gatgccaaga
      541 aacgacagcc tgtgacaacg gatctcatca aacgaatctt tttgtatctc caccccgagg
      601 agggagacct aaaaacactg aaataccgca aagatgtgcc gcagcatcgg ctgtactttc
      661 atgagtatgc accgccggac aagatcgcct acgcggttcg ccaagtggtg gaatggataa
      721 acgatccaga aacgcgacga acacggacga cgcttcgtat tgcggcgcga gcgcattacg
      781 atcttttgcg gattttcccc ttcagccagg acagcggcaa ggtgtcgaga ctgctgatga
      841 acttcctgct gcttcgcgct ggttatccgc ccgcgatcat tcattccacg gaacgacagc
      901 gttattacga agcgctcaaa ggggcacctc tatccattac ccacatggtg gaagaagcgc
      961 tggagaactc gattgcaagc atcgagaagt accttgacga gcgcacgacc cgaattcgct
     1021 ccttcgtatc ctgacttttc accccatgag aatcgactga accccttgac aggtgcgccc
     1081 ccatgcgtca gggtgccgcc tcttggaatt gtacggggca tcgtcccacc cacgaggatt
     1141 cgcagtcatg gcacggagcc agatcaccgg taaacgccgg atgctcgcaa acaacgtttc
     1201 gcactccaac atcaagacca aacgatggca actcgtcaac gtccagcgtc gtcgtatctg
     1261 ggtccctgaa ctcaatcggt tcgtacgcct cgatctcacg acccgcgacc ttcggaccat
     1321 cgatcgcatc ggccttgtgg cattcgccaa aaagcacggc tacaagctct gatcgatcac
     1381 aacaacccga gcttttcggt gtcctctatt tgatctcgct cgctcgcgga gccccagagc
     1441 ctgtcgctcg aacgatggcg aagacacgtc gttcagggtg attgtcaaca agagcggctc
     1501 gcagcgattg ttcgagcgat agatcggctt tcacacggtc catcacgatc tgccaatcca
     1561 ccgcttcgac tttgtggatt cccacggcag cccaaggtgt tcgaccctcc atcaataaag
     1621 cgatgggaat gggcaatcga atgggcgaac caggacgaac ccgcgggatg cctcgacgta
     1681 cgggcacggc gagcctcgac acggtgactt gatggggagc gacttccgga ccttgtggat
     1741 cgaacgcaca atgcatcacg gccagcacac cacgcggatc gctcgccatc atcatcaagc
     1801 acgtcagctc gggaacatca cccaaaaggt tcggctgacg catggatgac accttgcatt
     1861 cgacaaggct cgagaatgct tgcctgcacg tcggctagat cttgttgtgt aacgtttccc
     1921 ccctcatgcc ttccaccgat ttcgagcagt gcccgaacga aagacgattc gcgcaacgcg
     1981 acgggaccag catcaccgac ccgtcgaagc aaatgcgctc gacccgccgc gcgagccgct
     2041 tgcgcaaggc gtacgcccgg cgtggcaagt tccgacatcg ggatggagcc accataggcg
     2101 tggacggctg aaagcatggc ccctgttgcc gcaacaggga tgcgagcgga tacagggatg
     2161 aggtcatctt gctgaaagcc gcgagggcgt ggcgcgccaa cgccgggctg aacgaccgag
     2221 ccatcgaaaa gatgttcgcc cactcccgtg cctgcaacca acagcgaaac acagccgaga
     2281 agcacacctg gatgtgcccc cgcggcagta aaaaatccgg ttatgcaagc atcgaccgac
     2341 gttccccctt tggcgagaac catgcgcgca gactcggtca tgcttggctc cgaacccagc
     2401 gcaatggcag cccgtgatgt cattcctgct cctcgtcttc cgcatgcaaa gcatactcga
     2461 catgccgagt tctcgagatg cgaggttccg ctcccctccc ctctgatgca tggatggctt
     2521 tggccactgc caacggcgaa tcggtttcga cgatcacaaa agcttcgcga aacgcttgta
     2581 atcgctcaat ggttttgtcg attcgtgttt gcgacagacg acgggagtct cccaaaccac
     2641 gcaaacgcgt gtaagcgacg gaaccaggcg cgaggggctc tcgagcggca tctcgtacca
     2701 ataccagtcc caagcgtgat gccacgttcc gcgcttcttc ttcttcccaa agtcctggcg
     2761 gttcccaagc tagtcgaacg acatcgtgag gaattcgctg cacgagttct tccaaccgct
     2821 ttcgattcgc agcggtcggt gtaacagata ccggatggct gatgagcatc accggagact
     2881 cgaggattgt cgcgatttcg agcgtttgtt gcagagcgcg atcggcggca gcgctgggac
     2941 ggagttcggt gacgacattc ggcaacacga cactgaacac gaaagaaggc ggaacagatt
     3001 ttcgccatcg ccgtagcgtc ctgctcgcca aaggcacctc aggctccgtt cggacctcga
     3061 ggaggttgaa tttttttgcg tagcgctcga gcgcaccttg cagggtcctg gttccaatat
     3121 atacgcgctg catgaggaag gcgggttgtg ccaccgcatc gccacgaggg gaagggtgaa
     3181 taagggaaaa tcatgcttgg tttggggatc cccccacggt catcgttcga tcaaaacgtg
     3241 cctggggaag cgggatacac gttccagacc caattatcca acaagacgag agagtcccac
     3301 acaccatcgg acgtatccca cagtgcgata cggatctcca tgatttgtcc cggcgcaacg
     3361 tttccgacgg tggtgagcca gcccgttcct cctcccacca tattgctcct cccgcatcca
     3421 cctaggctgg tatcatcgaa tccggtgccc tgtaagaacg ttggaccgct caagcacggg
     3481 gcaaatccag atgtcgaccc ggagcatcca atgggccctg ccttgcatac gctaaacagg
     3541 ttttgcgcct gtggatcgtt ggcaaggttc actccaattg ggaaaacctt cggcggcgac
     3601 gtgacgcggt agaccgccaa gttcttgtcc gccggattgg gccactctgg ctgtggtgaa
     3661 tcgataagca ccacgaaaaa gtcgttgaag gcagaacata cgtactcggg gaactctgcg
     3721 gagaagaacc aggaagcaaa ggaaaatgac ctagcattcg tgggaacacg gattcgcaaa
     3781 cgaagcatga tcgggtcatt cgcatgatcg ccactcggtt caggacaccc cggcgcattc
     3841 ggaagccgat agccgttggc agcataccaa tcctgcggga atggactctt tttgccaaag
     3901 tcctgtccgg gctggaacgg agcataattc gggttcgctt ggtttacgga agccgcatgc
     3961 cccgtggata gtatcgcaag ccgcccaccg ttcaacgatg tgatcaaatt gccgaaacca
     4021 cttcgtatcg cacgcgattg gcttgcgggt acccccgtcc cattcgctaa cgaaaactca
     4081 ccggagatga ccccccaccg acggaaaggc ggcgggtccg tttccttcgt ttgctggcac
     4141 aacccgatcg ctttcgcata atccatagga tcggacgagt tcgtgttgtt caacatcccg
     4201 tcgcaaggag gctgtatgtt gtcgatgaga ccatcgcagt catcatcgac gccgttgccg
     4261 atgtattcaa atgctcctgg attcacgaga gccggcgtgg tcgaacagaa tcccggtttg
     4321 tcacagcaat ctccctccgc taccgtccaa ccgtcgccat cgcaatcatc gtggggacca
     4381 ccctcgcaga caggacccgt cccacccgtt ccgccatagc cgcctgttgc acccgtgcca
     4441 ccagcaccac ctatgccacc agcaccacct gtgccacctg tgccgcccga accaccgtag
     4501 ccgccacctc cgtagccacc cgtgccccca taaccgc
//
LOCUS       DAOSOR010000487         4535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_19191_length_4535_cov_4.494196, whole genome shotgun sequence.
ACCESSION   DAOSOR010000487 DAOSOR010000000
VERSION     DAOSOR010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19191_length_4535_cov_4.494196"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PK710_20205"
     CDS             complement(<1..463)
                     /locus_tag="PK710_20205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12110.1"
                     /translation="MSNDNHPNLAKAYLEELCQRLDRKEKLVRRREWWKMPWIASAAV
                     GLALSGCGGTVEAEDCGPNGDACGTENVDKGDKGKDTDAAPPVKEVCDNRVDDDGDGA
                     VDCDDDDCLKDPSCMISAEYAAPFEVEVCDNQVDDDGDGAVDCDDDDCLKDP"
     gene            694..3288
                     /locus_tag="PK710_20210"
     CDS             694..3288
                     /locus_tag="PK710_20210"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT12111.1"
                     /translation="MSPPTKLTLVLLGASIQRETELPGYESLSDFASENYNLALASLE
                     ASVRNTSDLTNHFDVHRIDLDISQGTQTLGMDVVHHIVALEPDLVGLSCYCWSIDALV
                     DVAEEIHQRANDVFLFAGGPSAGPVAKKLLLERPFISAVARGEGENTLIALLRARRNH
                     QSLHTVQGLTWRASDGVLHENPDGEPVDLSTLPSAYQLGILRPPSTSMLLETSRGCPF
                     QCRFCSCMGANRRLRYVPAHRVEEQLRWAVEHGIRGIKFADTAINFQTSRLRELTRAI
                     HRADPHQTLRFTYFLKPERLTLEQIDLLAQIPSDEVIIGTESLTPAARALAGKPPFSP
                     ETFTEQLSWLQRVGPVTCSFILGLPGDSPEGLEQTLSWICEFDDNHPGYLHVICLFWL
                     AVLPGSGLHARHNELGLRCSPRGTPYLLQSHEHSPDALLTMARQAIERHFAHPKLRVE
                     HFHKEYLAQDAPAPDRTIPILRVEHDTRPTVLFVGELDESFRDFGVTPYHLRGAQLRA
                     FLEKHNDIRQAYRFETALESENIVERFDNLHPQWLIASCLRLPPSPWLESLLTQQKIP
                     RLLLEGASSLAQAEAWMTAIPSAYAATFGESEIALRALLRGQPAPGLVTRQPHGLVRS
                     DSPQTLADLDDIPSPFQWGFIRRPGSTIAMQLGRPSPPRTWSDERIFRDMRWALEQRH
                     THVVWLDDSLPSDPSRLGTWIDTLRRADPQGSIRHSYRLDGTQSIACLKALRALPTHT
                     LTVTATVDPLWLSEAEGIAHAWGQKIRNLSKSHLLSIDQLMRKLAPLRRSDALAGWSP
                     GPWHTQDQNARLEFFWKRDTLVHIRLSTQDNRIEASFEVRGERPPEEQLDRLRRVITR
                     LLSGKQIA"
     gene            complement(3302..>4535)
                     /locus_tag="PK710_20215"
     CDS             complement(3302..>4535)
                     /locus_tag="PK710_20215"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT12112.1"
                     /translation="AGVTWRDEQGRVVEELDRAAADLSDLPSPLLEGVLTPPRQNLMF
                     EFSRGCIYRCTYCAWKGQGPGMRFVSDERVGHEVGWAVEHDYEHAFIIDSAINHDDSR
                     LERFSAQIARADPTRHLAFSYFVNHSLLSKDQARALARLRPHEITVGLETVNVRAARA
                     AGRRPVDRDEFIRAMDLLSEVAPATLSIMLGMPGDDLEGFRSTLDFVAEVAERKGKPR
                     VRMARVHWMLIAPGSKMWSLADRHGLVIAQPGVPYVLGSSTFSRDQLIAALHAMREHP
                     RSDLFVWEDAEPLGMLDPALPSMFVAGGDHIGGRVARQISQTEVLQAIRPLEPGRWLR
                     HEWRVGAFEHRHGWPVVVLEGPGNKRVMLQIRPRDAEPRPLARTRRFDLVWLPSGGEG
                     PEQHRLVKALVELIRSNE"
BASE COUNT          995 a         1563 c         1063 g          914 t
ORIGIN      
        1 tcggatcttt gagacaatca tcatcatcac aatccaccgc cccatcccca tcatcatcca
       61 cctgattgtc acagacctcg acctcaaaag gcgccgcgta ctccgccgaa atcatacaac
      121 tcggatcttt gagacaatca tcatcatcac aatccaccgc cccatcccca tcatcgtcca
      181 cccgattgtc acatacttct ttcacaggag gcgccgcatc cgtatcctta cccttgtcgc
      241 ccttgtcgac gttttcagtt ccacaggcat cgccattggg gccacaatct tccgcttcca
      301 cggtgccccc gcagcctgat aaggccaacc ctaccgcagc ggacgcgatc cagggcatct
      361 tccaccattc acgacgtcga accagctttt ccttgcggtc gagacgctga cagagttcct
      421 ccaaataggc tttcgcgagg ttcggatggt tatcgttcga catcacactc ctccattggc
      481 acaatagaga gaactgcaaa ccccgttcca tttttaacta catgtattaa catatctttt
      541 tgataatctt tcgattggtt tggatggtca tggcgcagca ttgacacaag ttggctcgaa
      601 ccccgagcca attccctctg ccctccacca tggtagcatc gaaaaaacca tgctcctgta
      661 taccgaagcc gccgcccgcg caaaccgcca ccgatgagcc cccccaccaa actcaccctt
      721 gtgctcctgg gagcctccat tcaacgggaa accgaactgc cgggttatga atcgctctcg
      781 gacttcgctt ccgagaatta caacctcgcg ctcgcttctc tcgaagccag cgttcgaaac
      841 accagcgatc tcacaaatca tttcgacgtt catcgcatcg atctcgacat ctcccagggc
      901 acgcaaacgc tgggcatgga tgtcgttcac cacatcgtcg ctctcgagcc ggacctcgtg
      961 ggtctgtctt gttattgttg gagcatcgac gctcttgtcg atgtggccga agagattcac
     1021 caacgcgcaa acgacgtttt tctgtttgcc gggggccctt ccgcgggccc tgtcgcaaaa
     1081 aaactgctcc tagaacgtcc attcatttcc gccgtggcgc gaggcgaagg ggaaaacacg
     1141 ctcattgcct tgttgcgagc acgacgaaac caccaatccc ttcacaccgt acaaggtctg
     1201 acctggcgag cctctgacgg cgtattgcac gaaaaccccg atggggaacc ggtggatttg
     1261 tccaccttgc cctcggcgta tcaactcgga atcctgcgcc cgccttccac gtcgatgctg
     1321 ctggaaacca gccggggatg cccgtttcaa tgccgtttct gctcttgcat gggggcaaac
     1381 cggcggttgc gctacgtgcc cgcccaccgt gtggaagagc aactccgctg ggccgtggaa
     1441 catggtattc gcggcatcaa gttcgccgac accgccatca atttccagac cagccgattg
     1501 cgcgaactca cccgggccat ccaccgggcg gaccctcacc aaaccttgcg gtttacctat
     1561 tttctaaaac ccgaacggct caccttggag cagatcgacc tgctggccca aatcccttcc
     1621 gacgaggtca tcatcggcac ggaaagcctg acccccgccg cccgagcgct cgcgggaaaa
     1681 cccccgtttt cccccgaaac gttcaccgaa cagctctcgt ggctccaacg agtcggcccc
     1741 gtcacctgtt cgttcatcct cggactgcca ggcgattcgc ccgaaggact cgagcaaacc
     1801 ctgtcgtgga tttgcgaatt cgacgacaac catccaggct atctccatgt gatttgcctt
     1861 ttctggctcg cggtacttcc cggttccgga ctccatgcgc gacacaacga acttggcctg
     1921 cgatgctccc cccgcggaac cccctacctg ctccaaagcc acgaacacag ccccgacgcg
     1981 ctcctcacca tggcacgaca ggccatcgaa cgacacttcg cacaccccaa actccgcgtc
     2041 gaacacttcc acaaagaata cctcgcccaa gatgcccctg ccccagaccg caccatcccc
     2101 atcctccggg tcgaacacga tactcgtccc accgtccttt tcgtcggtga attggatgaa
     2161 tccttccgcg acttcggtgt gaccccctac catcttcgag gtgcccaact ccgcgctttt
     2221 cttgaaaaac acaacgatat tcgccaggcc taccgattcg aaacagccct cgaatccgaa
     2281 aatatcgtcg aacgattcga caatctccac ccccaatggc tcattgcctc ctgcctccga
     2341 ctccccccct ccccgtggtt ggagtcgttg ctgacccagc aaaagatacc ccgcttgctt
     2401 ctcgaaggcg cctcatcgct tgcccaggcc gaagcctgga tgactgccat ccccagcgca
     2461 tacgcggcca ccttcggtga aagcgaaatc gcattgcgcg ctcttcttcg tggacaaccg
     2521 gctcccggcc tcgtcacgcg acagccccat ggcctcgtaa gaagcgattc accacaaaca
     2581 ctagccgacc tcgacgacat tccttccccc tttcaatggg gattcatccg gcgccccggc
     2641 tccaccatcg ccatgcaact cggtcgccct tcaccccctc gaacctggag cgacgagcgg
     2701 atttttcgcg atatgagatg ggccctggaa cagcgtcaca cccacgtcgt ctggctcgat
     2761 gacagcctcc cctccgatcc ttcccgcctt ggcacgtgga tcgatacgct tcgacgagcc
     2821 gatccgcaag gaagcatccg acactcctat cggttggatg gcacacaatc catcgcctgt
     2881 ttgaaagccc ttcgagcctt acccacccat accttgaccg tgacggctac cgtggacccc
     2941 ctctggctct cagaagcgga aggtatcgcg catgcatggg gacaaaaaat tcgtaaccta
     3001 tcgaaatcac acctgttatc catagatcaa ctcatgcgaa aactggctcc tttgcgccgt
     3061 tccgatgccc ttgccggatg gtccccggga ccatggcaca cccaagatca gaacgcgcga
     3121 ttagagtttt tttggaaacg cgataccctc gtccacatcc gtctttccac ccaagacaat
     3181 cggatcgaag cctcgttcga agtgcgcggc gaacgacccc ccgaagaaca actcgatcga
     3241 ttgcgccggg tcatcacgcg tcttttgtcc ggaaagcaaa tcgcctgaac gatcgtcgtg
     3301 gctattcgtt gctgcgaatc aactccacca gcgctttcac caatcgatgc tgctctggtc
     3361 cctcgccacc ggatggcaac cagacgaggt cgaaacgccg tgttctcgcc aaaggacgag
     3421 gctccgcatc tctgggccga atttgcagca taacacgttt gttcccaggg ccttccaaca
     3481 ccacgaccgg ccaaccgtgc cgatgctcga aggcgccaac cctccattca tgacgaagcc
     3541 atcggcccgg ctcgagggga cgaatggcct gaaggacttc ggtctgtgaa atttgtctgg
     3601 cgactcttcc gccaatatgg tcgccaccgg ccacgaacat ggaaggcaag gcaggatcga
     3661 gcatgccaag aggttccgca tcttcccata cgaacaaatc cgaacgcgga tgctcacgca
     3721 tcgcatgcaa ggcggcgatg agctgatccc gagaaaacgt cgaagatcca agcacatagg
     3781 ggacacccgg ttgcgcaatc acaagcccgt ggcggtcggc caacgaccac atcttcgagc
     3841 ctggagcaat gagcatccaa tgcaccctcg ccattcgaac ccttggtttt ccttttcgct
     3901 ccgccacctc cgccacgaaa tccaacgtac tgcgaaaacc ctcgagatcg tcgcccggca
     3961 tgccaagcat gatcgacagg gtcgcgggcg ctacctccga cagcaaatcc atcgcgcgaa
     4021 tgaattcatc gcggtccacc ggcctgcggc ctgccgctcg ggcagctcgc acgttgacgg
     4081 tttcgagccc caccgtgatc tcatggggtc gaagacgtgc caaggcgcgt gcttggtctt
     4141 tcgagagcag cgaatggttc acgaaatacg aaaacgccag atgtcgcgta ggatcggcac
     4201 gggcgatctg cgcggaaaat ctttccagcc gggagtcgtc atgattgatg gcgctatcga
     4261 tgatgaaggc atgctcataa tcatgctcca ccgcccagcc cacctcatga cctacgcgtt
     4321 cgtcggaaac gaagcgcatc ccgggcccct gccccttcca agcacaataa gtacatcgat
     4381 atatgcaccc tcgggaaaac tcgaacatca ggttctgacg cggtggggtc aacacaccct
     4441 cgaggagcgg ggaaggcaag tcggataaat ccgcggcagc gcgatccaat tcctcgacca
     4501 cccgcccttg ctcgtcccgc catgtcactc cagca
//
LOCUS       DAOSOR010000488         4525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_19239_length_4525_cov_4.047204, whole genome shotgun sequence.
ACCESSION   DAOSOR010000488 DAOSOR010000000
VERSION     DAOSOR010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19239_length_4525_cov_4.047204"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PK710_20220"
     CDS             <1..480
                     /locus_tag="PK710_20220"
                     /inference="COORDINATES: protein motif:HMM:NF014886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HOT12113.1"
                     /translation="ESAMGRYPVESVTMLARIATEMERERTMPSLQVVTHELDNQARN
                     LIASSVYHTIQDGSPVAIAVPSKTGGMARAIARFRPSVWIVAFSTHHATCQALQFSYG
                     VYPITVQEIPANWSEFLRSWLADHGLRKGIAVVTQGPTERDPSANHRLEVLELDPVV"
     gene            486..1043
                     /locus_tag="PK710_20225"
     CDS             486..1043
                     /locus_tag="PK710_20225"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOT12114.1"
                     /translation="MTLLAFDHQLFLTLHEALSHRLVLWFFFAITWLGHGLVLALLIV
                     PLMAWRDRPSLRTHLLPMIIAVATTGAVTNIAKIIVGRERPAVWAAQHALQVHTPFGY
                     PSDKSFPSGHAQTAFGTAVYLSLLYPKATPLFLTLACLVGLSRISLGAHFPGDVVVGA
                     LIGSFGSWIGYRWVLRKRSKPSPIS"
     gene            complement(1129..3534)
                     /locus_tag="PK710_20230"
     CDS             complement(1129..3534)
                     /locus_tag="PK710_20230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12115.1"
                     /translation="MRWSARLASVFSSSVFSTIRSVALGLAILASVVLIGVRVHAHYP
                     VQEWLFWRYLSYWGWMALLSVSCSSVGHRVMSWIFPRRLPLGEHLVLSFALGVFVFFV
                     GMFLLGVSGALGGISALVLPLLLLASGAFPLGRSVLRVIRHLRAWRARPRPSLSLWRY
                     PILALGLLSIAALYFVLISPANISYDARWYHLSIAEHYAAYGGIDRFDEGWFQGTNPH
                     LGSWLYTWAFTLPGTRLFDRAMLCAHLEFLLFLWTLASVSPLVRRLLHGVRSPLAWVA
                     MFLFPGLFLYDSNLSVGADHVGAFWAIPIYLAVMRAWSKPSPSRLSLAAIFVSGALLS
                     KYTVIILAVGPILALIARTLWVGTAALLRKSKDVAKDAWLGIGAFALTGLVATAPHWL
                     KNWLWHGDPVYPILYRHLAPRPWTDDSAMFYSIGIEGRLISPDGSVIDRIWQTIKIVF
                     TFSFVPHNWPRFHGEVPVFGFLFTVSTCLLLFLRRSQRTWGLVFITWIGLATWASIHA
                     RDRYLQSILPWMVVVTAVVLVRIWKSHWSMRALLGLLVGLQLVWGGDVYFFRTHAMIH
                     DSPVKAAVDFLATGFEKKYDERLKIFGAMEKIADHIPKKAKVLMHEEHLTLGLQRRRV
                     NDWQGYQGGLAYGLMTDPKELHEQLVTWGVTHVVWKDAKSAAVDSVGGDLLFFDWVLR
                     HTQDRQVEGGYRIAMLPTEAPQGPFHDEVAYLTCGTNYAQGLYHRSALHLADRVEDRK
                     YPNPDILLEPDASNAQELIERAQYVVWNAKCRPEVRGAWLSAFERMARRGTATDLYVR
                     KKE"
     gene            3625..4320
                     /locus_tag="PK710_20235"
     CDS             3625..4320
                     /locus_tag="PK710_20235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT12116.1"
                     /translation="MAVQYDEQKQYWEGIRERRAPTHPAVLAFARHKLDIMLKTLPQG
                     SSRPKTMLEVGAGNGFFSHTFATAFDLTCLDFSQNMLDRNPLPADRKVQGDAEHLPFD
                     DDSFDIVFCGNLLHHLEEPIIAVREMARVARSFVVLVEPNAMNPLMFAFGVGKPAERG
                     TLKFTRRYVRKLGEQAGLKARRMVTQGAVLPNKTPSLLVSPVSRLEIPNPFGFYHIAI
                     FETPGNAVVGGAE"
BASE COUNT         1050 a         1347 c         1218 g          910 t
ORIGIN      
        1 gagtccgcca tgggacggta tcccgtcgaa tcggtaacga tgctggctcg aatcgccacg
       61 gaaatggaac gggagcgcac gatgccctcg ttgcaagtgg tcacccacga actcgacaat
      121 caagcccgaa atctcattgc cagcagtgtg taccacacga ttcaagacgg ctctcccgtg
      181 gctatcgcgg tcccgtccaa gaccggcggg atggcgcgag ccatcgcacg ttttcgtcca
      241 tccgtgtgga tcgtggcgtt cagcacccac catgccacat gccaggctct ccaattttcc
      301 tacggcgtgt atccgattac ggtgcaagaa atccccgcga attggtccga gtttttgcgg
      361 agctggcttg cggatcatgg cctgcgaaaa ggaattgcgg tggtcacaca aggtcccacc
      421 gaacgagatc ccagcgccaa tcatcgtctc gaagtgttgg aactcgaccc tgtcgtctga
      481 gggccatgac gctccttgct ttcgatcatc agctattttt gaccctgcac gaagcgcttt
      541 cccatcgtct cgtgctctgg tttttttttg cgatcacctg gctcggtcat gggcttgtcc
      601 ttgccttgct catcgttcct ctcatggcgt ggcgtgatcg cccttcgcta cgcacgcatc
      661 tgctaccgat gatcatcgcc gtggcgacca ccggtgccgt gaccaatatc gcgaagatca
      721 tcgtgggtcg cgaacgccct gcagtctggg ccgcgcaaca cgcgctgcaa gtccataccc
      781 cctttggata cccgagcgac aagtcctttc cctccggaca tgcgcaaacc gctttcggca
      841 cggcggtata tctttcgctc ctctatccca aagcgacgcc tctgtttctc acgctcgcct
      901 gcctcgttgg cctatcgcgt atcagcctcg gcgctcattt ccctggtgat gtcgtcgttg
      961 gcgctctcat cggctcgttc ggctcgtgga tcggttatcg ttgggtgctg cgaaaacgct
     1021 cgaaaccatc ccccatctcc tagcaccggc aacgaatcct cttcggatga ttcgccgccg
     1081 catcgtgccc cgaaacgagg cccgtgcttt tcatcgagcg cttgggattc attctttttt
     1141 cctcacgtac aagtcggtag ccgtaccgcg tctcgccatg cgttcgaacg ccgacagcca
     1201 cgcccccctg acctccggcc ggcatttggc attccaaacc acatactgcg cacgctcaat
     1261 caattcttgt gcattggaag cgtcgggctc gagaagtata tccggattgg gatacttcct
     1321 gtcctccacc cgatctgcaa gatgcagcgc cgaacgatga tacagtcctt gcgcgtagtt
     1381 cgttccacaa gtcaaatacg ccacttcatc gtgaaaaggt ccttgcggag cctcggtcgg
     1441 aagcatcgcg attcgataac ccccttctac ctgccggtcc tgggtatggc gcaagaccca
     1501 atcgaaaaac aataagtccc cccctacgct atcgaccgcg gcggacttgg cgtctttcca
     1561 caccacatgg gtcactcccc atgtcacgag ttgctcgtgc aactccttcg gatccgtcat
     1621 cagaccatag gccaatcccc cttgataccc ctgccaatcg ttcacacggc gacgttgcaa
     1681 gccgagcgtc agatgctcct catgcatcaa taccttcgcc tttttcggta tatgatcggc
     1741 gatcttttcc atggcgccaa agatcttcaa tcgctcgtcg tatttttttt cgaaacccgt
     1801 ggccagaaaa tccaccgcag ccttgacagg gctatcgtga atcatcgcgt gcgtgcggaa
     1861 aaaatacaca tccccgcccc acacgagttg caaccccaca agcaatccca atagcgcgcg
     1921 catactccaa tggcttttcc aaatacgaac cagcacgacc gccgtcacca ccaccatcca
     1981 cggcaggatg ctctgcagat accgatctcg tgcgtggatc gaagcccatg tggccaatcc
     2041 gatccaggtg atgaaaacca gtccccaggt tctttgggat cgacgaagaa acagaagcaa
     2101 gcaggtgctc acggtgaaca aaaatccgaa cacaggcact tcaccgtgaa aacgcggcca
     2161 gttatgcggc acgaaggaaa acgtaaatac gatcttgatg gtctgccaaa tcctgtcgat
     2221 gacggatcca tcgggagaga tcaggcgccc ttcgataccg atcgaataga acatcgcgga
     2281 atcgtcggtc caaggacgcg gcgcgagatg acgatacaaa atgggataca cggggtctcc
     2341 atgccatagc cagtttttga gccaatgcgg cgcggtggcc acaagccccg tgagagcgaa
     2401 agcgccgata cccaaccagg cgtctttggc cacatctttc gattttcgca gcagcgccgc
     2461 ggtgccaacc catagagttc tagcgatcaa cgcaaggatg ggaccgacgg ccaaaatgat
     2521 gacggtatat ttggatagca aggcacccga tacgaaaatg gccgcgagag acaatcgcga
     2581 agggctgggc tttgaccagg ctcgcataac ggccaggtaa atcggaatgg cccaaaacgc
     2641 gcccacatga tcggccccaa ccgagagatt cgaatcgtag agaaataaac cgggaaataa
     2701 gaacatggcc acccaagcca agggtgagcg aaccccatga agaagacgtc gcaccagggg
     2761 cgaaaccgag gccaatgtcc aaagaaacag cagaaattca agatgcgcgc agagcattgc
     2821 gcgatcgaat aaccgcgtgc ccggcagcgt gaacgcccac gtataaagcc agctccccag
     2881 gtgcgggttg gtcccttgaa accagccttc gtcgaagcga tcgatcccac cgtaagcggc
     2941 gtaatgctcc gcgatggaaa gatgatacca acgggcgtcg taggagatat tcgccggtga
     3001 aatgagcaca aaatacagcg ctgctatcga aagcaagccc agagcaagga tggggtaacg
     3061 ccacaaggaa agcgagggcc gtggtcgcgc ccgccaagcg cgaaggtgac gaatcacccg
     3121 caggaccgag cgtcctagag gaaacgctcc gcttgcgagc aaaagcagcg gcaaaaccag
     3181 cgcggaaatc cctccgagcg ccccggacac gccgagcaag aacatcccca cgaaaaacac
     3241 gaaaaccccg agggcgaagc tcaacacaag atgctccccc agcggcagac ggcgcggaaa
     3301 aatccaggac atcacccgat gaccgacgct cgagcacgat accgaaagca gcgccatcca
     3361 cccccaatag gaaaggtaac gccaaaacag ccattcttgc accgggtaat gggcatggac
     3421 gcgcacacca atgagcacca ccgaggccaa aatggcgagt ccgagggcga ccgagcggat
     3481 cgtcgagaaa accgaagagg aaaaaactga agccaggcgg gcagaccatc tcatgggcgt
     3541 tggcggttgt agcacccttt ggggcgtgtc gtcttggcct ccacggtctt gcacaggtca
     3601 atggcaagag atagaagcga tgggatggcg gtccaatacg atgagcaaaa gcaatattgg
     3661 gaggggattc gcgagcgacg ggcccccacc catcccgcgg tcctcgcgtt tgcccggcat
     3721 aagctggata ttatgctgaa gacgttgccc caggggtcgt ctcgcccgaa gacgatgctc
     3781 gaggtgggcg cgggcaatgg tttcttttcc catacgttcg cgacggcttt cgacctgacg
     3841 tgtctggatt tttctcagaa catgctcgat cgaaatccct taccggcgga tcgcaaggtg
     3901 cagggggatg cggagcacct gccgttcgac gacgattctt tcgatatcgt attttgcggc
     3961 aatcttttgc atcatctgga agaacccatc atcgcggtac gagagatggc gcgtgtggcg
     4021 cggtcgttcg tggtgcttgt cgagcccaac gccatgaatc ccttgatgtt cgctttcggc
     4081 gtagggaagc cagcggaacg aggaaccttg aagtttacgc gtcgttatgt gcgaaagctt
     4141 ggtgagcaag cgggattgaa ggctcgtcgg atggtaaccc agggggcggt attgcccaat
     4201 aagacaccat ccctgctggt ttccccggta tcgcggctgg aaattccgaa tccctttggg
     4261 ttttatcata ttgccatctt cgagacgccc ggcaacgctg tcgtcggcgg ggcggagtaa
     4321 gcttccgagc agtcaactgg gcactgttcg tgcctgacct ccaggagcat tccactgcca
     4381 cgttgttgtt tcgaaggttg tgtgtattct attccgcggc ttacgagacg tgggagtaac
     4441 aagcggatat tgcttagatg tttgtgctat tagtactgct gccggaaatt tggcccacct
     4501 gaaaatactt cgataagcgc ttgcg
//
LOCUS       DAOSOR010000489         4507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_19331_length_4507_cov_4.941599, whole genome shotgun sequence.
ACCESSION   DAOSOR010000489 DAOSOR010000000
VERSION     DAOSOR010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19331_length_4507_cov_4.941599"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PK710_20240"
     CDS             <1..768
                     /locus_tag="PK710_20240"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOT12117.1"
                     /translation="RLLRLAQLCFSLDIPFEYEDAADPKRLGAFLRDVQDHPLAGIIG
                     ILTLRSLTPASYDTVNVGHYGLASHAYAHFTSPIRRYPDLVNHRIVKARLRADRFQAT
                     DWHDIAMHCTRREREVVDVEREVFDVYRCVLMRDRIGERYSGIVTDVGMNAVTVTLDQ
                     PFVEILIPEEGLGSGGYQRSEDGLHWVARRSGDRISLGTRLEVEVVSTDLTRRVIIGK
                     RIFNTKTFKKLSTTPISSRKNVREYRKRRKYNDKSNR"
     gene            999..1328
                     /locus_tag="PK710_20245"
     CDS             999..1328
                     /locus_tag="PK710_20245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOT12118.1"
                     /translation="MAKAKTAAKKAMTKSALIQTIADLLGEASSRKQVKATIEALQDI
                     GHKELKKNGVFVLPGFAKFVVVKKAARPARQGINPFTKEPMTFAAKPASKAVKARPVK
                     AIKVAVS"
     gene            complement(1456..2127)
                     /locus_tag="PK710_20250"
     CDS             complement(1456..2127)
                     /locus_tag="PK710_20250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT12119.1"
                     /translation="MPLNDRDLATEQQIPQSEDNDDRLSSAVHAVLEGVGAEISASFP
                     GITRLGGQIVAVLYLADGPRSMDTLAEELGRSKSNIFTNLKALEGAGIVERRRVAGLR
                     HDTYALTGPYPDVIIGAYLGRLRRVVADKRKLTQRALMLLGDAPGPQADSLRRRLLDL
                     QRKYELFGRMFSLFGTAIDGPVDLEALLSSIPGKTLEMFVDMARMTLGKLSRSGESGG
                     EGKEE"
     gene            complement(2170..2868)
                     /gene="amrA"
                     /locus_tag="PK710_20255"
     CDS             complement(2170..2868)
                     /gene="amrA"
                     /locus_tag="PK710_20255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOT12120.1"
                     /translation="MGRWSWPWGLMLAVVGSVEIGCRCAGDDPVANSGYGSVVDSRDF
                     DLTAEEKQALLTLARTSVETWVRDHQRVPTDELVRKFPKLNTQRACFVTLRRDGQLRG
                     CIGSLEPYRALVQDVRDNAVSAAMHDTRFRPVEASELSSLRYSLSVLDMPRPLQGVPP
                     SQLPQYLSQHRPGVIIELRGRRSTFLPSVWEDLPDAKIFLSKLCMKQGAPSDCWMDAQ
                     ARISTYGSINFGEP"
     gene            complement(2900..4102)
                     /gene="amrS"
                     /locus_tag="PK710_20260"
     CDS             complement(2900..4102)
                     /gene="amrS"
                     /locus_tag="PK710_20260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOT12121.1"
                     /translation="MAQLSRCGKKVQQQAMTDRRRFLKRTASTFGLGWMGYEGYEQFV
                     RGRAPSNGALVFRNDAPLPLDEYSRRAEYWTLRGRDVECLLCPHGCVLGEGDRGFCRT
                     RVVDGGVMHTVAYGNLCAFAVDPVEKKPLYHFLPQTPIVSVAMAGCNLRCPNCQNWQI
                     SQAKPEEVMRHPLLPEALVTLTKQRSIPSIAFTYTEPMVCFEYVRDTASLAREAGIKN
                     VLVTAGYVNEEPVRELAKVVDAVQLDVKGFDEAVYRGLARGRLAPVLRTLALLWEQGV
                     WVEVSWLMVSEWTDKPQDVATFAKWIVSHLGAQVPLHLLRFHPAHRMTHTPPTSVASM
                     QRAWELAKDSGLQHVYLGNVPSLGGGQTRCPHDGQLLIERRGYQVVKNRLKNHACPAC
                     GTRLAGVF"
     gene            complement(4214..>4507)
                     /locus_tag="PK710_20265"
     CDS             complement(4214..>4507)
                     /locus_tag="PK710_20265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12122.1"
                     /translation="GKLLPFGAAKLLWRLKVRGPRSARLIILGIRQKLRQIRRYAALS
                     TYMYVEMHRSSQQLHLQEGELSWTLEDNSPVNVAIRFMGGKKYKTMRLYERSL"
BASE COUNT         1094 a         1355 c         1091 g          967 t
ORIGIN      
        1 cggcttctgc gtctggcaca gctatgtttt tccctggata ttccctttga atacgaggat
       61 gcggcagatc ccaagcgcct gggagccttt ctccgtgacg tacaggatca tcctttggcg
      121 ggaatcatcg gcatactcac ccttcgctct ctgacgccgg cctcgtacga tacggtgaat
      181 gttggtcact atggtttagc ctcgcatgcg tacgcccact tcacgtcgcc cattcgtcgc
      241 tatccggatt tggtcaatca tcggatcgtg aaagcgcggt tgagggccga tcgatttcag
      301 gcgacggact ggcatgacat tgccatgcat tgcacgcggc gcgaacgtga ggtcgtcgat
      361 gtcgaacggg aggtattcga tgtatatcgc tgtgtattga tgcgagatcg aattggcgaa
      421 cgatattcag gtatcgttac ggatgtggga atgaacgcgg tcacggtgac gttggaccag
      481 ccattcgtcg aaatcctcat cccagaggag gggttgggca gcggaggtta ccaacgaagt
      541 gaagatggtc ttcattgggt ggcgcgaagg tccggggacc ggatttcgtt ggggactcgt
      601 ctggaagtcg aggtggtatc cacggattta acgcgtcgcg tcattatcgg caaaagaata
      661 ttcaatacta agacattcaa gaaattatcc accaccccaa tttcatcgcg aaagaacgtc
      721 cgcgagtatc gtaagcgtcg gaaatataat gataaatcaa atcgttgaac atcgaatttc
      781 ttatatataa tccatattat ttgcaattaa tcctacccca taaactgctg cacagacgtt
      841 gcccccaggt accgaaaccc cttctagcgt aggataaaat cgtcatctcc caacccggta
      901 atgcgtttgt ccccttgatt acgggaaaaa accgtggtct agcttgagta cactgcaaag
      961 accgtgcagg cacacggtca attgaaggag aatgtgacat ggcgaaggca aagacagcag
     1021 cgaagaaggc aatgacgaag agtgcgctga ttcagacaat cgcggatctg ctgggcgagg
     1081 cttcttcacg caaacaagtg aaagccacca tcgaagcgct tcaagacatc ggccacaaag
     1141 aactcaagaa gaacggcgtg ttcgtgctcc cgggttttgc taagttcgtc gtggtcaaga
     1201 aggccgcgcg tcccgcacgc cagggcatca accccttcac caaagagccc atgacgtttg
     1261 ctgcaaagcc tgcaagcaaa gcggtcaaag ctcgtcccgt caaggcaatc aaggttgccg
     1321 tttcctgatt gaccccccca taaactcgaa cggcgcgtca tggacgcgcc gttttctttt
     1381 gtccgcctta tccctctttc tcactcgcac catctgacca tgcatatgtt gcagcatgtg
     1441 tcaacgtacg gctcatcact cctctttccc ctctcctcct gactcaccgg atcgggatag
     1501 cttcccaagc gtcatccgag ccatgtccac aaacatctcc agcgtcttac caggtatcga
     1561 actcaacaac gcctccaaat ccaccggacc atcgatcgcg gtcccaaaca acgaaaacat
     1621 tcgtccaaat agctcgtatt ttcgctgtaa atcaagcaat cggcgacgca acgaatccgc
     1681 ttgcggtcct ggtgcatccc ccaacaacat cagcgctcgc tgcgtcagct tacgcttgtc
     1741 cgccaccacg cgacgaagac gtcccaagta cgctccgatg atgacatccg gatagggtcc
     1801 ggtcagcgca tacgtatcgt ggcgcagtcc tgcaacacgt cgacgttcta cgataccggc
     1861 tccttcgagg gctttaaggt tcgtgaaaat attgctcttc gaacgtccca actcctcggc
     1921 cagcgtatcc atcgaacgag gaccgtccgc caagtacaac acggcgacaa tctgcccccc
     1981 caaccgagtt attcctggaa acgatgcgga aatctcagct cccactcctt ccaataccgc
     2041 gtggacggcg gatgacaatc gatcatcgtt atcctcgctt tgcggtatct gctgttccgt
     2101 cgccaagtca cgatcgttca agggcacgtc ccacgctcct gataagggtg cgttccagtt
     2161 tcgaggctct catggctcgc caaaattgat cgaaccatac gtactgatac gagcctgcgc
     2221 gtccatccag caatccgatg gtgcgccctg tttcatgcat aatttcgata aaaatatctt
     2281 tgcatccgga agatcctccc acaccgatgg aaggaaagtc gaacgacgac ctcgaagctc
     2341 gatgatgact cccgggcgat gctgtgacag atactgcgga agctgcgatg gtggcacgcc
     2401 ttgcaacgga cggggcatat ccaatacgga tagggaatac cgcaaagacg atagttccga
     2461 tgcctccacg ggacgaaaac gtgtgtcatg catggcagcc gataccgcat tgtcgcgtac
     2521 gtcttgcacc aaagctcgat aaggctccaa gctgccaatg cagcctcgaa gctgaccgtc
     2581 gcgacgcaac gtcacaaaac aagcgcgttg cgtgttcaat tttgggaatt ttcgaaccaa
     2641 ctcgtcggtc ggaacccgct ggtgatcacg cacccacgtc tccacactcg tgcgagcaag
     2701 cgttagcaat gcttgttttt cttccgccgt cagatcgaag tcacgactgt ccacgaccga
     2761 accgtacccg gaatttgcca ccgggtcatc ccccgcgcaa cggcacccga tttccacgga
     2821 accgaccacc gcaagcatca acccccatgg ccatgaccat cgccccatga acaacctcga
     2881 aacgctgtca aatactctat caaaacactc ccgcaagacg tgtgccgcac gcgggacatg
     2941 catggttttt caatcgattt ttgaccactt gatagccgcg ccgttcgatg agcaattgcc
     3001 catcgtgggg acagcgggtt tgtccgcccc ctaaggacgg tacatttccc aaatacacgt
     3061 gttgaaggcc ggagtctttc gccaactccc aagctcgttg catcgaagcc accgatgtgg
     3121 ggggtgtatg cgtcatgcga tgggctggat ggaatcgcaa caaatgaagg ggaacttgcg
     3181 ctccaagatg ggacacgatc cacttggcaa acgtcgccac atcttgcggc ttatctgtcc
     3241 actcactgac catcaaccaa ctcacctcga cccacacacc ctgttcccat aacagcgcca
     3301 gcgttcgaag gacgggtgcc aaccggccac gggcaagccc ccgataaacc gcctcatcaa
     3361 acccttttac gtcgagctga accgcatcta ccactttcgc caactcacga acaggctcct
     3421 cattgacata tcccgccgtc accaacacgt tttttatccc cgcctcccga gcaagactcg
     3481 ccgtatcccg cacatactcg aaacaaacca tcggctcggt atacgtaaac gcaatcgatg
     3541 gaatactccg ttgcttggtg agcgttacca acgcctccgg cagcagcgga tggcgcatca
     3601 cctcttcggg tttggcctgc gaaatttgcc aattttgaca attaggacat cgtaagttgc
     3661 atcctgccat ggcgaccgaa acaatcggag tttgcggtaa gaagtgatag agcggcttct
     3721 tttccacggg atcgacagca aaagcgcata aatttccgta ggccaccgtg tgcatcacgc
     3781 caccgtcgac cacacgcgtt cgacaaaatc cgcgatctcc ttccccgagc acgcaaccat
     3841 ggggacacaa aaggcactcc acatcccgcc ctcgtaacgt ccagtactcg gctcgccgac
     3901 tgtattcatc caaaggcaaa ggagcatcgt tgcggaacac caatgccccg ttcgaaggcg
     3961 cacgcccacg tacgaattgc tcgtatccct cgtatcccat ccaaccaagg ccaaacgtcg
     4021 aagcggttcg tttgagaaag cgtcgacgat cggtcatggc ctgctgttgc acctttttcc
     4081 cacaacggga caactgtgcc acgactgcct ttgcgatgca cccacggaac cagcacgcac
     4141 cctcgtggat ccatcgaatg gaataacgtt tttctcaccc gtccgcgcga aaacctcgct
     4201 cgcgcgcaaa cggtcacagg gagcgttcgt acaagcgcat ggttttatat ttttttccac
     4261 ccatgaagcg gatggcaacg ttgaccggtg agttatcctc caacgtccag gacaactcgc
     4321 cttcttgaag atgcaattgc tggctcgaac gatgcatttc cacgtacata taggtggata
     4381 atgccgcgta acgacgaatt tgacggagct tctggcggat accgaggatg atcagacgcg
     4441 ccgagcgcgg accgcgcacc ttcaatcgcc acagcagctt ggcagcaccg aacggcaaca
     4501 acttacc
//
LOCUS       DAOSOR010000490         4504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_19343_length_4504_cov_3.863790, whole genome shotgun sequence.
ACCESSION   DAOSOR010000490 DAOSOR010000000
VERSION     DAOSOR010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19343_length_4504_cov_3.863790"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="PK710_20270"
     CDS             complement(<1..976)
                     /locus_tag="PK710_20270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12123.1"
                     /translation="MIRLSAYPWHSLDRVPRDGLRLVRDGRIALGTTGLMQALEKAAE
                     DYLAPDPVRITVQGTRHGPIAPSVITENVICLFANPPQTLIALVVVEPELAHRIVSTV
                     LMRPILWINRSQPIPSMLHAAFGAFVLSCLRTAAADVGFQLLSVGPSAQASFAALHQP
                     FGILDTTVTLARAAYGASVWWHTKARPTAGTLPFGRKQLLSMGNIPLSLEIVAAMSTI
                     SRGQLASLGRGDAWLPADGWTVRYREARLEGEVLLMSPHATIGWSGQLRHDGTIELGA
                     RKVQEMNEQQATTPSPNSLDTMEALADVPVVVRVEVGTLTLTAREWASV"
     gene            complement(1150..2814)
                     /locus_tag="PK710_20275"
     CDS             complement(1150..2814)
                     /locus_tag="PK710_20275"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing serine/threonine-protein
                     kinase"
                     /protein_id="HOT12124.1"
                     /translation="MSYSFVVSSGPRAGLRLPLPNQQAVILGRAADVDLLLPQEDRFV
                     SRHHARVEVQADGVYLHDLAGRKTTSVNGMPVRWARLAPGNVVRLGNTLLRLVYDGAQ
                     EQGSETVIQPEISRQPVLSPASSLTPPRGISTTTWESKADVLSCDRCGAAGSAPPSPD
                     LWDAAYLCPDCRQQRRKLPSDLPNRIGRFQVLGKIYQGGTGILLEAVSEDGIHVVIKM
                     LRGTNVAKKAMDRFVREQRIATVLRHPNIVRCYEVGAHENHPYLVSEYVSGGSALDIA
                     GREQPVQDVLWLGADLFRALGYAHDLGIVHRDVSPSNVLLTGPDMGAGVRAKLADFGL
                     AKSRIDLNPMDITVAGEAGGSTLTMGPEQVRNFIQMTPSADIYSAAATVFWMLTRDTP
                     LVLPCPLQDAPMHVRKEAILHPDRRSLARLRPDVPKPVVELIDSLVAHSSTAREQLSA
                     KQIAESLGQLAETVARAQIVMTPDVPDSTRRAIHRADIPPDRSPWRFRLSSISSIYWI
                     VMWKWRRPTFARPSIPKTPIASTAPSSVINVFNKTWMTAWRDGKSW"
     gene            complement(2992..4185)
                     /locus_tag="PK710_20280"
     CDS             complement(2992..4185)
                     /locus_tag="PK710_20280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HOT12125.1"
                     /translation="MKRNRPTVAIVGAGPAGSAAAFVTASHGLRTILIDRARHPREKV
                     CGEGCTPRAVRNLERMGLDLTLQGVSVTHTHLISPGGIELFTQLPPGIHGGQALVIPR
                     TILDERLTQRAVRAGADLRQDLRVDQVTVSSSCARLHLHDGSTLDADVVIGCDGMPSV
                     VRRSLGGEPFRQAQTASAVRAIYEDVVWDRERALVLVWDKAVLPAYGWIFPLPEGRAN
                     VGIGMRMDQLQAKEANLRRILETFLQLPRIRSLLAKARPVGRVKGHPLPMTCSPTDFV
                     FDRGLLAGDAAGFVNPLTGEGIEYALESGELAGRVVVQAAQLGNFDRRSLMLYSWACA
                     DQFLPILRLNGWLRHVFAVPWLLDRLFRASLRSERLREDIARIALGGEGARFSGRMLR
                     GALFG"
BASE COUNT         1001 a         1310 c         1284 g          909 t
ORIGIN      
        1 gaacgctcgc ccattctcgt gctgtcaagg tgagcgtgcc aacctccaca cgaaccacca
       61 ccggaacgtc tgcaagcgct tccatcgtat ccagtgagtt cggagaaggg gtggttgctt
      121 gctgttcgtt catttcttgc accttccttg ccccaagctc gattgtgcca tcgtgtcgta
      181 attgtccact ccaaccgatt gtcgcatggg gcgacatcaa caatacttct ccttcgagcc
      241 gtgcttcgcg ataccgcacg gtccatccat cggcgggtag ccaagcatcc cctcgaccca
      301 aacttgccaa ttgtcctcgc gaaatcgtgg acatcgccgc aacgatttcg agcgacaagg
      361 gaatgttacc catggataga agctgctttc gaccgaacgg cagcgtgccg gccgtgggac
      421 gcgctttcgt atgccaccac accgaagcgc cataagctgc ccgagccaag gttaccgtcg
      481 tatcaaggat tccaaaaggt tgatggagcg cggcgaaact cgcttgtgcc gaaggaccga
      541 cagataagag ctgaaaccca acgtcagccg ctgccgtccg aagacacgaa agaacgaaag
      601 caccaaatgc cgcatggagc atcgatggaa tgggttgtga gcgattgatc cacaagatgg
      661 gacgcatcaa cacggtggat acgatgcgat gggcaagctc cggctcgacg accaccaatg
      721 cgattagggt ttgcggtgga ttggcgaaaa ggcagatcac gttttccgtg atcacagatg
      781 gggcgatggg tccgtgccgc gttccttgca cggtgattcg aaccgggtct ggagcaaggt
      841 agtcttctgc tgctttctcc aacgcttgca tcaacccggt tgtaccaagg gcaattcgcc
      901 catcgcgaac cagccgcaac ccatcgcgag gcactcgatc gagcgaatgc cacgggtagg
      961 cagagaggcg aatcattcct tgattgtcgt cagttttgaa gcgttcgtac agagccccgg
     1021 tacaaatcct gtctgggttg ttggcatggt ttcgagcact gattgttggt tgtggagaag
     1081 ggttgtttcc catcccatga acgcgcctgc atgcagatgt gggaaaaacc gtgtcgacct
     1141 aggacaggtt caccaactct tcccatcgcg ccaagccgtc atccacgtct tgttgaagac
     1201 gttgatgacg ctcgagggcg cggttgatgc gatcggggtc ttgggaatcg atggccgcgc
     1261 gaacgtcggc cgccgccatt tccacatgac gatccagtag atggacgaaa tactcgagag
     1321 tcgaaagcgc cagggagagc ggtcgggcgg aatatcggcg cgatggatgg cgcgtcgtgt
     1381 gctgtcggga acatccggtg tcatgacgat ttgtgcccga gctacggttt cggcgagctg
     1441 cccgagagac tcggcgattt gtttggcgga caattgttct cgcgccgtcg aggaatgggc
     1501 aacgaggctg tcgatgagtt cgactaccgg tttgggtaca tctgggcgaa ggcgtgcgag
     1561 ggaacgacga tccggatgaa ggattgcttc tttgcgaaca tgcatcggtg catcttgcaa
     1621 cggacacgga agaaccagcg gggtgtcgcg ggtgagcatc cagaacaccg tggccgccgc
     1681 tgagtaaata tcggcggagg gcgtcatttg gatgaagtta cgcacttgct cgggtcccat
     1741 cgtcaaggtc gaaccccccg cttctcccgc aaccgtaata tccattggat tcaagtcgat
     1801 acgactcttg gcaaggccga agtccgcgag cttcgcacgc actcccgcgc ccatatcggg
     1861 gcccgtcagc aatacgttgg aaggagatac gtcgcgatgc acgataccaa gatcgtgggc
     1921 gtacccaaga gcgcggaaca aatcggctcc gagccagagc acgtcttgca cgggttgctc
     1981 acgccccgcg atgtccaagg cgcttccccc actgacatac tccgaaacca gataaggatg
     2041 gttttcgtgc gctcccacct cgtagcaacg gacgatattg ggatgacgta atacggtagc
     2101 gatacgctgt tcgcgcacga agcggtccat cgcttttttc gccacgttgg ttcctcgaag
     2161 cattttgatg acaacgtgga tgccatcctc ggaaacggct tcgagaagaa taccggtgcc
     2221 tccctgatag attttgccaa gcacctgaaa acgaccgatg cgattgggca aatccgaagg
     2281 cagctttcgc ctctgctgtc gacaatccgg gcataagtac gcggcatccc ataagtccgg
     2341 cgacggagga gccgagccag ccgctccaca gcgatcgcat gacaatacgt ccgccttcga
     2401 ttcccacgtt gtcgtcgaaa taccccgcgg tggggtcaac gatgaagctg gcgaaagaac
     2461 cggttgcctc gatatttcgg gctggatgac ggtttcgctc ccttgctcct gcgcgccatc
     2521 gtaaacgagt cgaagcaagg tattgccgag acgtaccaca ttgcctggag caagacgcgc
     2581 ccagcgtacg ggcattccat tgaccgatgt tgtttttctc cctgctagat cgtgcaagta
     2641 aacaccatct gcctgcactt ctacccgtgc atgatgccgt gacacgaacc gatcttcttg
     2701 gggtagtagt aagtcgacat ccgcggcgcg accgagaatg acggcttgtt ggttgggcag
     2761 aggcagacgc aagccggccc gtggaccaga ggaaacgacg aagctgtagg acatggtcga
     2821 tccagcgcgg ctgagggaaa acgagccccc gagccatcct gctataccac cgatgatgaa
     2881 gcgcatcgat ggtctgcaag cggcattttc cgcttggaat tttgtaccga tggttcaatg
     2941 cccaagagaa gggagcgtaa cgtatttttg aggtgcgaac ctggcgatcg cttatccgaa
     3001 taacgccccc cgcagcattc gaccggaaaa tcgagcgccc tcgccgccaa gagcaatccg
     3061 agcgatatcc tcccgcagtc gttccgaacg caaggacgct cgaaaaagcc tgtccaataa
     3121 ccatggcacc gcgaacacat gcctcaacca gccgttgagc cgcaggatgg gtaggaattg
     3181 gtccgcgcac gcccacgagt aaagcatcaa cgaacgacga tcgaaatttc cgagctgggc
     3241 ggcttgaacc accacacgtc cggcaagttc tccggactcc aaggcgtatt cgattccttc
     3301 gcccgtcaat ggattgacga atcctgcggc gtcgcctgcc agcaacccac gatcgaaaac
     3361 gaaatccgtg ggcgaacaag tcataggcag aggatgacct ttgacccgcc ccaccgggcg
     3421 ggcttttgcc aacagacttc gtatccgtgg caattgtaag aaggtttcga gtatccggcg
     3481 taggttggct tccttcgcct gaagttgatc cattcgcatt ccaatgccca cgttggccct
     3541 gccttccggc aaaggaaata tccaaccata cgcggggagc accgccttat cccacaccaa
     3601 gaccaacgcg cgctctcgat cccaaaccac atcttcgtaa atcgctcgca ccgcgcttgc
     3661 cgtttgcgct tgccgaaacg gttcgccccc caaagagcgc cgaacgaccg aaggcatccc
     3721 atcacatcca atcacgacat cggcgtccaa cgtagacccg tcgtgcaggt gcagacgagc
     3781 gcaggaagaa gacacggtga cttggtcgac gcgaaggtcc tgtcgaaggt cggctcccgc
     3841 gcgaacggca cgttgcgtga ggcgttcatc aaggatggtt cgagggatga cgagcgcctg
     3901 gccgccatga atgccagggg gaagttgggt gaacaattcg attcccccag gagaaatgag
     3961 atgcgtgtgc gtgacggaaa caccttggag cgtcaggtcc aaacccatgc gttcaaggtt
     4021 ccgaacagct cggggcgtgc atccttcccc gcagactttc tcgcgaggat gccgcgcccg
     4081 gtcgatgagg atcgtacgca atccatgcga tgcggtaaca aaagccgccg cggaccccgc
     4141 gggtccagca ccgacaatcg cgaccgttgg cctgttgcgc ttcatggtga ttcgcatatc
     4201 cacaccgtct gccgaaccgt ggatgccgct tatcctgtct caggatttca tcgtcgccta
     4261 gacgcatttc cgctccgccc gatggtcctt ggatttgcgt aaacttgccc acctttcgag
     4321 gatttcgata cagccaggga cacatgcacg acgtggaact gcagcacgcc tggaagacct
     4381 tgttcggacc atcgattcat ccagcggtct tgaatgaaac tgctgtgcgt cgcgcgtacc
     4441 gacgtcgcgt gctcgagatt catcctgatg tggcgggagc gggtgccaca ccccagtttt
     4501 tgcg
//
LOCUS       DAOSOR010000491         4500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_19360_length_4500_cov_4.613498, whole genome shotgun sequence.
ACCESSION   DAOSOR010000491 DAOSOR010000000
VERSION     DAOSOR010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19360_length_4500_cov_4.613498"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>4500
                     /locus_tag="PK710_20285"
     CDS             <1..>4500
                     /locus_tag="PK710_20285"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT12126.1"
                     /translation="CWALGAVLYELLSGSPPYNEQSNMLQLLRVCSADPAPRLAPFEA
                     VPEPLVELIARCLEKDPAKRPQSHEIAQDLHSMLHEGRLRLEAEQSPFRGLLPFSEDQ
                     ADFFYGRDDEIASMLERIRHEPLLPVVGPSGAGKSSFVQAGLIPRLREHGRWIVLRLR
                     PGREPFEALAIRLTRGEHDGTNRSVHTTSRGGGSEEDTREKERALSVQLFENPPFLSF
                     SLHQIAEQENAGVLLFVDQLEEIYTLASNPDVRRRFMTAVCGAADDVESRVRVVLTLR
                     DDFLVRLAETPVVREALGHVTVLRTPGPEALRDIVMTPVRLAGYQFEDQSLVEEMIET
                     VGAQAGALPLLQVAAARWWEGRDRKKKILTKSAYEAMGGVVGAIVKQADGVLDGLTPR
                     QLRVAREMCLRLVTPEHTRRLVVREELLEGLGSDGEEVLRRMVLGRIVVARRGRSEKG
                     GDVEFVHESLVSHWTQLVRWLEGSREEIAFVAEVGAAAELWDKRGRPKEEVWRGPSLH
                     AALAKASQIPTMPERVRAFLEAGQRRERRGSRRRRVFAGVAFGVMTLAATILAWQGQE
                     AASQRRVAEKEREIAELQRWRAEERHAEAMREGAEAAILRDNMLEARAKIRVSLEAQD
                     TPSSRALWSQLRHQPLQAVVRLPTRAWTAKFMDSGRSVAALDIQGSVHTIDVATTQTR
                     RLADWNATSVAEASFSQDGRFLVSTNKTGDLRVWDLEHRSVAKLGHPPPRATWFAISP
                     NGQTVASLHATGAIHLWDVRSGSQTKTFDNQGLEVTRATFDPLAPRLASGDVAGNIRI
                     WTLTTGESKTLPQRHDKLVSCLAFDAHGRLLATSDWGGQIRLWDSRSGKLLRTLEGHV
                     GAVYKVRFHPDGRRLVSVSGDKTVRIWRIDDGSLLHVLRAHQDQVYDVDFDPTGETLM
                     TLDGLGALRLWSLQAASGTIRYDGHRGSVFGIAIHPNEHQLASASRDSSIRIWNVTTG
                     HVEAVLEGHEAGVLGVDYSPDGTRLVSGGNDNTVRLWDTHRQVQLAVLSGHRDAIVHP
                     VFSPDGKLIASPSNDGTIRIWSAATGDKIHELVGHTGGVNQASFSPDGRVLATGADDA
                     TIRLWDVQTAKPLRVLRGHQSPVYGPRFGASEHLLVSAGWDGDTRVWTLPSGESRVVH
                     RCEGYAFFPTFDASQHHVGATCADGTAAIVDLKSNLGLTLQGHVGHVTDMAYAPSGRW
                     VATSGDDGTVRLWSATKGLPVWRGIMLRQEPAEAWSHEGGVSLDGQPVVEKAWQKALQ
                     SQASIASSSRDTLCYLSRDGMLKRWDLKADTQSWQHPMPHGIKVMAHESRCFVLQSDG
                     EASLWNDRGERRTVASDATAIGVRQGEGLVAVSGKILRTALDGTVTSQIPVDERISAL
                     AYVGTYLVAGTYQGDIRFLMDENTRDQPRGSLPGLPSASVQALLEGPAETVVAGFSNG
                     MVGVWDGRRRALVATEHLHGKVLFLQRNRGKVHALSELGDRVTLDLELLERPYCEVMR
                     DVWDRV"
BASE COUNT          857 a         1204 c         1483 g          956 t
ORIGIN      
        1 ttgctgggct ctgggtgcgg tgctgtacga acttctcagc ggctcgcctc cctacaacga
       61 acaatccaat atgttgcagt tgcttcgcgt gtgttccgcg gaccctgctc cacgcctggc
      121 gcccttcgaa gcagtgcccg aaccgctcgt cgaattgatt gcccgttgcc tcgaaaaaga
      181 cccggcgaaa cgtccgcaat cccatgaaat tgcccaggat ttacattcca tgctgcatga
      241 aggcaggctt cgcttggagg cggaacagtc gccgtttcga ggcttgcttc ccttttctga
      301 agaccaggca gacttctttt atgggcgcga tgatgaaatc gcgtcgatgc tcgaacgcat
      361 tcgacatgag ccgctgttac ctgtcgttgg cccttctgga gcaggcaaaa gctctttcgt
      421 ccaggcgggc ctgatcccaa gactacgaga acatggccgg tggatcgtgc ttcgcctccg
      481 tcccggtcgt gaacctttcg aagcgttggc cattcgtttg acccgcggcg agcatgacgg
      541 gaccaatcgc agcgttcata ccaccagtcg ggggggggga tccgaggaag atacaaggga
      601 aaaagaacgc gcgttgagtg tgcaactatt tgaaaatccg ccgtttttat ccttttccct
      661 ccatcagatc gccgaacagg aaaacgccgg agtcttgttg ttcgtcgacc agctcgagga
      721 gatctatacg ctggcgagca acccggatgt gcgtcgccgt ttcatgacgg ccgtgtgcgg
      781 tgcggccgat gacgtcgaga gccgtgtgcg ggtggtgttg acgcttcggg acgattttct
      841 ggttcggttg gcggagacgc cggtggttcg ggaagcgctt gggcacgtga cggtattgcg
      901 gactccgggg ccggaagcgc ttcgggacat tgtgatgacg ccggttcgtc ttgctggcta
      961 tcagttcgaa gatcagtcgt tggtggagga gatgatcgag acggtggggg ctcaagcggg
     1021 cgcgcttcct ctgctgcaag tcgcggccgc ccggtggtgg gaggggcggg atcgaaagaa
     1081 gaaaatcctt accaaaagcg cgtatgaggc catgggggga gtggtaggag cgattgtgaa
     1141 gcaagccgat ggcgtgctcg atgggctcac gccccggcaa ttgcgggtgg cgcgggagat
     1201 gtgtttgcgc ctggtcacgc cggagcatac gcgtcgcctg gtcgttcgcg aggaattgct
     1261 cgagggactt gggtcggatg gggaagaggt cttgcgtcgc atggtgctgg ggcggattgt
     1321 ggttgcgcga agggggcgtt ccgaaaaggg aggcgatgtg gaattcgtcc acgagtcgct
     1381 ggtgagtcat tggacgcaat tggtgcgatg gttggaaggg agccgggagg agatcgcgtt
     1441 tgtagcggaa gtcggcgcgg cggcggagtt gtgggacaag cgaggcaggc cgaaagaaga
     1501 ggtttggcgg gggccatcgc tgcacgctgc gcttgccaaa gccagccaga taccgacgat
     1561 gcccgagcgg gtgcgggctt ttctcgaagc gggtcagcgc cgagagcgtc ggggatctcg
     1621 aagacgccgg gtcttcgcgg gtgtggcctt tggtgtgatg acgcttgcgg ccaccatcct
     1681 tgcctggcaa ggccaggagg ccgcctccca gcgccgtgtg gcggaaaaag aaagagaaat
     1741 cgctgagttg cagcgatggc gggcagagga aaggcacgcg gaagccatga gggagggggc
     1801 ggaagccgcc attcttcgcg acaatatgct cgaagcacgg gccaaaatcc gcgtttccct
     1861 cgaggcccaa gacactccct cgtctcgcgc tttgtggagt cagttgcggc atcaaccctt
     1921 acaagcggtc gttcgacttc ccacacgcgc ctggaccgcc aagttcatgg actcgggacg
     1981 ttcggtggcg gccctcgata tccagggctc cgttcacacc atcgatgtgg ctactaccca
     2041 aacccgtcgc ctggcggatt ggaacgccac cagtgtggcg gaagcttctt tctcccaaga
     2101 tggtcgcttt ctcgtttcca cgaacaagac cggtgacttg cgggtgtggg acctggagca
     2161 tcgatccgtt gccaaactcg gccatccccc cccgcgcgcc acgtggttcg ccatatcccc
     2221 aaatggacag acagtcgctt ctttgcacgc cacgggggcc atccatcttt gggacgttcg
     2281 ttccggatct caaacaaaaa cctttgataa tcaagggctt gaagtgacgc gggccacctt
     2341 cgatccgttg gccccgcggc tggccagcgg cgacgttgcc gggaatatac gtatctggac
     2401 tctgaccacc ggagagagca aaaccttgcc ccagcgtcac gacaagctcg tatcctgtct
     2461 cgctttcgat gcccatggcc gcctgctggc gacgtcggat tggggcggac agatacggct
     2521 ttgggattcg cggtctggca aactcctccg tacgttggaa ggccatgtcg gtgcggtata
     2581 caaggttcgg tttcatcccg acggccgacg cttggtttcc gtaagtggtg ataaaaccgt
     2641 acgtatttgg cgaatcgacg acggctcttt gcttcatgtc ctccgggcgc accaagacca
     2701 ggtttatgat gtggacttcg atccaacggg tgaaaccctg atgacgttgg atgggcttgg
     2761 ggcgctgcgt ttatggtcgt tgcaggccgc ttcggggacg atacgatacg atgggcatcg
     2821 aggcagtgtg tttggcattg ccattcatcc gaacgagcat caactcgctt ccgcaagtcg
     2881 tgattcgtcg attcgtattt ggaacgtgac cacggggcat gtcgaagcgg tgctcgaagg
     2941 acacgaagca ggtgttctcg gtgtcgatta tagtcccgat ggcacgcggc tcgtttccgg
     3001 aggcaacgat aatacggtgc ggctgtggga tacgcatcga caggtgcaac tcgcggtgct
     3061 cagcggtcat cgggatgcca tcgtgcatcc tgttttcagt cccgatggca agctcattgc
     3121 ctctcccagc aacgatggca ctatccgtat atggtctgct gccacgggag acaagatcca
     3181 tgaattggtt ggccatacgg gaggtgtcaa ccaggccagc ttctctcctg atggccgagt
     3241 ccttgccacg ggcgcggacg atgccacgat tcgtttgtgg gacgtgcaaa cggcaaagcc
     3301 gctacgtgtg ctccgcggac accaatctcc cgtgtatgga ccccggtttg gagcgtcgga
     3361 acatcttttg gtctcggcgg gatgggatgg tgacacgcgg gtgtggacgc ttccttccgg
     3421 ggaaagccgt gtcgtgcatc gatgtgaagg atatgctttt ttccctactt tcgatgcatc
     3481 ccagcatcat gtgggggcca cctgcgccga tgggaccgcc gcgatcgtcg atctgaaaag
     3541 caatcttggg ctaacgcttc agggccatgt gggccacgtc accgacatgg cttacgcccc
     3601 ttccgggcga tgggtcgcaa cgtcgggcga cgacggcacc gtgcgactgt ggtcggctac
     3661 caaggggctg cccgtttggc gcggaatcat gttgcgacaa gagccggcgg aagcatggtc
     3721 ccacgaaggg ggggtatccc tcgacggaca gccggtcgtc gagaaagcct ggcaaaaagc
     3781 attgcaatca caggcttcga tcgcctccag ctcccgcgat acgctctgtt atctcagccg
     3841 tgatggcatg ctgaagcgct gggatctgaa ggccgatacg caaagctggc agcatcccat
     3901 gccccacgga atcaaggtga tggctcacga aagccgatgt ttcgtcctgc aaagcgatgg
     3961 cgaagcctcg ttgtggaacg atcggggcga gcgtcggacc gttgccagcg acgccacggc
     4021 gatcggggtt cggcaaggtg aggggctcgt ggccgtttcc ggaaaaattc tgcgaactgc
     4081 cttggatggg acggtaacga gccagattcc tgtcgatgag cgcatcagcg cgttggccta
     4141 cgtgggaacg tacttggtgg ccggcacata ccagggggat attcggtttc tgatggatga
     4201 gaacacgcgg gatcaaccga ggggaagcct tcccggactt ccttccgctt ccgtgcaggc
     4261 gttgttggaa gggccagcgg aaaccgtggt tgcgggattc agtaatggga tggttggcgt
     4321 gtgggatggt cgtcgacgcg cgctggtggc gacggagcat ctgcacggta aggtattgtt
     4381 tttacagcga aatcgtggaa aggtgcatgc cttgtccgag ctgggggacc gggtcacgtt
     4441 ggatctcgaa ctgctcgaac gaccatattg tgaagtcatg cgtgatgtat gggatcgtgt
//
LOCUS       DAOSOR010000492         4490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_19429_length_4490_cov_3.666967, whole genome shotgun sequence.
ACCESSION   DAOSOR010000492 DAOSOR010000000
VERSION     DAOSOR010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19429_length_4490_cov_3.666967"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1494)
                     /locus_tag="PK710_20290"
     CDS             complement(130..1494)
                     /locus_tag="PK710_20290"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOT12127.1"
                     /translation="MNTRVPFLAATLASFLFGACSSEDTSPAHKDPSPIENENNQPGK
                     VARSTLPRESEPQISEQAKIELVEGHNAFALQMYDLIRKDEGVGENMFFSPTSMLLAL
                     SMAYAGAQGDTANEMKNALHFALPPEQLAAGFNWLDQTLEGREEAAYKVAVSEAAKWD
                     LEPPSRDDFRLHIVNSIWGEAKMTFEQPFLDTLARHYDAGVFLSDFRNNPEVERVRIN
                     DWVAEETWDRIQDLLPPGTIDTMTRTVLVNAIHLKLPWDEQFRVVDDMSFHRLDGSTM
                     AVPTIANTKYWPYLEEDGLQAVRVPLKGGNIDLVIIAPAQGQLDAFESRLTTDGFASI
                     FDKMETREVALHLPKVKFTTPSVSLTSKLQIMGMNVHFTSSADFSGITKDESLFISDV
                     VHKAMLGIDEHGVEAAAATAVVMAGSGVPDPVDFDVDRPFFVGIVDKTNTVLFAGHIV
                     DPTK"
     gene            complement(1693..2667)
                     /locus_tag="PK710_20295"
     CDS             complement(1693..2667)
                     /locus_tag="PK710_20295"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF024801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HOT12128.1"
                     /translation="MGRLENGAWVADWYDTSRTGGQFVRSTTTFRHWITPDGSAGPSG
                     EGGFPAELGRYHLYVSLACPWAHRTLIYRVRKRLEEHISLSVVHPDMLEDGWTFATDF
                     PGTTGDTLYNLPFLRDIYTRVSPTISGRVTVPVLWDKKRHTIVSNESSEIIRMFNSAF
                     NHLTGNEEDDWPLELRDEIDAMNDRIYHGLNNGVYRAGFATSQQAYDGAVTQVFATLD
                     WLEERLKTHRYLMGERMTEADWRLFPTLMRFDSVYHLHFKCNRRRLIDYPNLWGYTRE
                     LYQKEGIARTVHLDHIVRHYHRSHTTINPYHIIPINPIIDFEAPHGRG"
     gene            complement(2732..3964)
                     /locus_tag="PK710_20300"
     CDS             complement(2732..3964)
                     /locus_tag="PK710_20300"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOT12129.1"
                     /translation="MNRSQEKELIDQIVRHLVQACPRRYPTSESEDKAQQLVRIYLEE
                     AGLRTEFHSFSFNASAAANVALHFGLGTVGTVLGGVAPRLAFALHAGSALSYYAESMH
                     RGYLLRRLFPHRPCRNVLGVLPSHKEPRLRVVFMAHTDAAFTGLIFDSRVVQAISHRL
                     PPGLGWLHRPLSLATRAHVALSGIDLLRSVVGPLAFPLRPLEFLLTLPSAAVFLASLE
                     VALRNEVVPGANDNLSGVAALPVLARRLAEDQPEDVEFVFVSTAAEEAMMGGADALAR
                     HVESSWDKAKTVLIVLDTLSLGSLRFLDPEGQIGPLPVPFWLRETVEAAAASEARFHD
                     VQAFRPPVGGTDAAPFLARGWDAVALTCIDPSLGSARHYHQMTDDPDHLDLDQVLHAV
                     DFAEALARRIVRDRSRAQ"
BASE COUNT         1072 a         1277 c         1232 g          909 t
ORIGIN      
        1 ccctcatttt ttcctgaatt ggaaagggat acgagctgag aatttttttt ggcgctggga
       61 aaaatgaggg gatggctcag cgccccgcct cgactaccgc gggaacgtga agctccccga
      121 gaacggtgat tacttggtag gatcgacgat atgtcccgcg aacagaacgg tattggtttt
      181 atccacgata cctacgaaaa atgggcgatc gacatcgaaa tccaccgggt ccggaacacc
      241 tgaacccgcc atgaccaccg ctgtggcagc cgcggcctcc acgccgtgtt cgtcgatgcc
      301 gagcatggct ttatgtacta cgtctgaaat gaacagggat tcatccttgg taatccctga
      361 aaagtcggcc gacgaggtga aatgaacgtt catccccatg atctgaagct tggatgtaag
      421 cgagacggat ggcgtggtga acttgacttt cggcaagtga agagccacct ctcgtgtctc
      481 catcttatcg aatatcgagg caaatccgtc ggtcgtcagc cgcgactcga atgcatcgag
      541 ttgaccttgg gccggagcta tgatgacgag atcgatattg ccgcctttga gaggaactcg
      601 tacggcttgc aggccgtctt cttccagata aggccaatat ttcgtattgg cgatggtagg
      661 aaccgccatc gtagagccat cgagtcgatg gaaagacata tcgtcgacga ctcggaactg
      721 ctcatcccac ggcagcttca agtgaatggc attgacgagc acggtgcgcg tcatggtgtc
      781 gatggttcct ggcggcagca gatcctgaat cctgtcccac gtctcttccg caacccaatc
      841 gttgatgcga acgcgctcga cttcagggtt gttgcggaaa tcggaaagga atacgcccgc
      901 atcatagtgc cgggccaagg tgtcgaggaa aggctgttcg aaggtcattt tcgcctcccc
      961 ccaaatggaa ttgacgatat gaagccgaaa atcgtcgcgc gacggaggct cgaggtccca
     1021 ctttgccgct tcggaaacag ctaccttata ggcggcttct tctcttcctt ccagagtctg
     1081 gtcgagccag ttgaagccag cggccaattg ttcgggggga agggcaaagt gcagcgcgtt
     1141 tttcatctcg ttggccgtgt ccccttgcgc tccagcatac gccatggaca gggcgagaag
     1201 catgctcgtg ggggaaaaga acatattctc cccaacacct tcgtccttgc ggatgagatc
     1261 gtacatctgc aaagcgaaag cattgtggcc ttcgacaagt tcgattttgg cctgctcgct
     1321 tatctgcggt tcgctttccc tcggtaaggt cgaacgggcg actttacccg gttggttatt
     1381 ttcgttttct atgggagacg ggtctttgtg ggcgggcgag gtatcctcgg aactgcaagc
     1441 accaaacaga aacgaagcca acgtggcagc tagaaaaggg actcgagtgt tcatgactgg
     1501 attctcctat cgggtcgaaa aagcctcgga gcaatcttgg tgccaaagcc gcttttcccc
     1561 atcaaagccc caaataccca gctccttgac cgaatggcgc agaaatcctc gtcgcggttg
     1621 gcacatattg gcaccaacct aggaaatggc aagcgtggag cctttctcgc tacgtgccat
     1681 acgtcgacgg cctcatcccc ggccgtgagg cgcctcgaaa tcgatgatcg gattgatagg
     1741 aatgatatgg taagggttga tggtggtatg gctcctatga taatgacgta cgatatggtc
     1801 cagatgaacc gtccgggcga ttccctcttt ctgataaagc tccctcgtat aaccccatag
     1861 gttgggataa tcgatgagtc gcctgcgatt gcacttgaag tgcaagtgat atacggaatc
     1921 gaaacgcata agggtgggaa atagccgcca gtccgcttcc gtcatccgct cgcccatcaa
     1981 gtagcgatgc gtcttcaggc gttcttccag ccagtcaagc gtggcaaata cctgggtcac
     2041 ggcgccatcg taggcttgtt gcgatgtcgc aaaacccgct cgatagactc cattgttcaa
     2101 tccatgatag atacgatcgt tcatggcatc gatctcgtcg cgcaattcca aaggccaatc
     2161 atcttcttca ttccccgtaa gatgattaaa cgcggagttg aacatgcgga tgatctcgga
     2221 ggattcgttg gaaacgatgg tatgccgctt tttatcccaa agaacgggca ccgtcacacg
     2281 gccggatatc gtgggggata ctcgcgtata aatatcacgt aaaaacggca ggttgtacaa
     2341 cgtatcccct gtcgttccgg ggaaatcggt agcaaacgtc caaccatctt caagcatgtc
     2401 gggatgaacg acggataagg aaatatgttc ctcgagtctc ttgcgcacgc ggtaaatcaa
     2461 ggtgcggtgc gcccaggggc acgccaggga tacatagaga tgataccgtc ccaactccgc
     2521 gggaaaaccg ccttcgcccg aaggccccgc agaaccatcc ggcgtgatcc aatgacggaa
     2581 tgtcgttgtg gaacgaacga attgcccacc ggttcggctg gtatcatacc aatccgcaac
     2641 ccatgctccg ttttcaagtc ttcccatgaa cctcaacgta gtgggctcga gccccaggca
     2701 ccacccttcc gcttcacaaa acgggctttt ttcattgggc tctgcttcga tcgcggacga
     2761 ttctgcgcgc gagggcctcg gcgaagtcca ccgcatgaag cacctgatcg aggtcgagat
     2821 ggtccggatc gtcggtcatt tggtgataat gacgcgcgga accgagcgat ggatcgatgc
     2881 aggtaagagc caccgcgtcc cagccccgag cgagaaaagg agcggcatcg gtgcccccca
     2941 caggcggacg aaacgcctgg acatcgtgaa aacgtgcctc acttgccgcc gcggcctcca
     3001 ccgtttcccg aagccaaaaa ggcacaggca acggcccaat ctgcccctcg ggatcgagga
     3061 aacgaagact tcccaggctt agggtgtcga gcacgataag cacggttttg gccttgtccc
     3121 acgatgattc cacgtgccgc gccaacgcat cggcgccacc catcatggct tcctccgcgg
     3181 cggtggaaac gaaaacgaat tccacatctt caggctgatc ttcggccaac cggcgggcaa
     3241 gaacgggcag cgctgccacg ccagaaaggt tgtcattggc gcctggaacc acttcgttgc
     3301 gcagcgccac ctccaaactg gcaaggaaaa ccgctgccga tggcaaggtc aacaggaatt
     3361 ccagcggccg taaaggaaaa gccagcgggc cgaccaccga ccgcagaaga tcgatccccg
     3421 aaagggcaac atgcgcgcgt gtcgcaagcg aaagcggtcg atgcagccac cccaatcccg
     3481 ggggcaaccg atggctgatg gcctgaacaa cccgtgaatc gaagatgaga ccagtaaaag
     3541 ccgcgtccgt atgagccatg aacaccacgc gcaaacgtgg ctccttgtgg gaaggaagca
     3601 cgccaaggac attgcggcat ggacgatgcg gaaaaaggcg ccgcagcaga tacccccgat
     3661 gcatcgactc cgcataatag gacagggccg aaccagcatg aagcgcaaac gccaaacgcg
     3721 gtgcgacacc tccaagcacc gtgcccacgg tgcctaatcc aaagtgcaac gccacattgg
     3781 ccgccgcgct tgcgttgaac gaaaaggagt gaaattccgt tcgtaagccc gcctcttcga
     3841 gatagatacg cacaagctgt tgcgccttgt cctccgattc gctcgtggga taccgtctcg
     3901 gacacgcttg caccagatgg cgaacgattt ggtcgataag ctctttttcc tggcttcggt
     3961 tcatgccatc ggcataccac aacgcgcaag cgaagccgct catcaaatca taagcccgcg
     4021 gacacatccc acgtaccacg ggccatgggc catgcgacaa ggaacgccta gatgccaagt
     4081 atgtgaaatt acaatacaat attcgtgttt cgatctccaa cgctatccgt ggggaccatc
     4141 aacgcccgca atcatggcag gccgacttgg gtgccacctt ccgagcggcc agcttcagca
     4201 gactcaatcg ctccccgcct atcacgacca tgagaggctg ttcagccgga gagcctgatc
     4261 cctcgctccg aagctggaca cattggcgta ccacacacga atagggcatg aattcagcgg
     4321 aattgagcag gtccaggatc agatgggctg cgtcatggga ccttcgggat gcgcagcttg
     4381 gaggagccta tttttcgctt ccccaaaaga agcccaacga gagcgaacca caagaattgc
     4441 ccgttcgccg tcttggaagg cgcggcgcga cacgcacatc cggaaccagc
//
LOCUS       DAOSOR010000493         4487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_19451_length_4487_cov_3.954874, whole genome shotgun sequence.
ACCESSION   DAOSOR010000493 DAOSOR010000000
VERSION     DAOSOR010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19451_length_4487_cov_3.954874"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /locus_tag="PK710_20305"
     CDS             complement(<1..1374)
                     /locus_tag="PK710_20305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12130.1"
                     /translation="MRRVFYCAVFLSFGVAACSSDSSEEYHNVPLTDANSDVAADAGS
                     DTNADSTVDTGPDGTEETSSDTASDTASDTFSDVVVTDTPEADLPDGNEPDVVSDSPT
                     DTPIDAPPPDGGSSGTNVIANPGFEYWSDGLPDAWKGEATNIGDDAISDIVTSPHGGA
                     HACALSNPSSTHKRFSTAAFSLADGYYDCTYWVRGAGEVRNATYNGSSYGTYSSYSTV
                     DSPNWKQISWGFNLHGDADAFELVFSVRSTNASSGNLQIDDVSCIRRPEPCDQVTCPE
                     WAACDPGTVACKPKYGRCGTASDCQSWQQCDGTNTCVLAPGRCNNTADCDVASSTPVC
                     DKATHTCVAGDPCAGVVCDEWKQCNPDTALCVLKPGRCNTTADCLHDLPACHGATHTC
                     MPSNHSSNIVPNGGFESWSVYYIPYEGDHLIPDGWYGLDIPGSTEIDPERVLPYSAHP
                     HTGALALQ"
     gene            1453..1794
                     /locus_tag="PK710_20310"
     CDS             1453..1794
                     /locus_tag="PK710_20310"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOT12131.1"
                     /translation="MKQRLNADMLALSRELSFPLLVMDLGRRVHFRVPTAARVDWHAR
                     GTCTHHVSTTRDWSRGGVFIRTDIPRAPGELLHVLMDVGEVIVQFEATVAHRCKDGMG
                     VRVDSSSVRIL"
     gene            1853..3817
                     /locus_tag="PK710_20315"
     CDS             1853..3817
                     /locus_tag="PK710_20315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOT12132.1"
                     /translation="MRRLLPVCCALCCLAPAYAFANPSFTGPVTMRDGVALATDVYLP
                     EGPGPFPVLVARTPYDKAGGQGIGDGLRDAGVVTVVQDTRGRFASQGVDCLFQCDGED
                     GFDTLAWVAAQPWCNGRVVTWGGSALGIVQYMAAVHAPPALDAMWVEVATPTVYQHAF
                     FQNGAFRQSMMEGWLAGQHSDFFLDTLAEHPLDDGTWDSVQTADRYDQVIVPGVHYGG
                     WYDIFNQGTIDAFVGYQHHGGPGAKGKQKLVMGPWTHALNTTQAGELTYPSNAAGVPG
                     GTDTMLVTWLAHYLGFELSSAAVDAIPTVQYYVMGDVKDPSAPGNTWRSADDWPIPAA
                     PVRMHLHPGGMLDESCPTVNDARTSYLYDPAFPSPTKGGANLLIPAGPVDQTIVENRS
                     DVIVFSSAVLTKPMEITGRIRASLWVETDAVDTDINVRLTDVYPDGRSMLVLDGVQRL
                     GYRNGSGSLEPVPIGQPIPVTVDLWSTSIILAAGHKLRVSITSSNAPRFWPNPNDGST
                     YRNTKSPTKANVSILHDQQHPSYVELPDPNRPDTDITVCDPHDAGAEASTDGSVTHDA
                     PHAEDGAIETGVDASVDAPLLEDALDTKEGGSSSAATAEGDGGCGCRTKTGSSATPWL
                     ALAWLFLSVVNMRKYSRKHVFRQANPHVQS"
     gene            complement(4218..>4487)
                     /locus_tag="PK710_20320"
     CDS             complement(4218..>4487)
                     /locus_tag="PK710_20320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12133.1"
                     /translation="LAAVITAHNRDEAVQACASDQEWAGIECVKKGEIQEQQAKCQGN
                     FGMLNLLQNTTDDEMGKEAGNQTQVLMKMMQQQACDWLDKHKQPH"
BASE COUNT          906 a         1286 c         1311 g          984 t
ORIGIN      
        1 ctgcaatgcc agcgcacccg tatggggatg ggcggaatac ggtaggacac gctccggatc
       61 gatctcggtc gaacccggaa tgtccaggcc ataccaccca tcgggaatca agtgatctcc
      121 ctcgtagggg atatagtaga ccgaccagct ttcgaaccca ccgttgggca cgatattcga
      181 tgagtgattc gagggcatgc acgtatgggt tgctccatgg catgccggca agtcgtgaag
      241 gcagtcggcc gtcgtgttgc accgacccgg tttcaacaca cataacgccg tgtcaggatt
      301 gcattgcttc cattcatcgc acacgacccc cgcacacgga tcgccggcca cgcacgtatg
      361 cgttgctttg tcgcatacgg gtgtgctcga agccacatcg caatccgccg tgttattgca
      421 gcgtcccggc gccagcacac acgtgttggt tccatcgcat tgttgccacg attggcaatc
      481 actcgcggtc ccacatcgac cgtatttcgg cttgcacgca acggtgcccg gatcacacgc
      541 ggcccactcc gggcacgtca cctgatcaca aggctccggt cgacgaatac aagacacatc
      601 gtcgatttga agattgcccg aggaggcatt cgtcgatcga accgaaaaaa cgagttcgaa
      661 tgcatcggca tcgccgtgga gattgaatcc ccacgagatc tgtttccaat tcgggctgtc
      721 gacggtcgaa taagaggaat aggttccgta gctgctgccg ttgtaggttg cgttgcgaac
      781 ttctccagct ccgcgaaccc aataggtgca gtcgtaatac ccatccgcca aagaaaaagc
      841 ggctgtcgaa aagcgtttgt gcgtggaact cgggttggaa agagcacaag catgggcgcc
      901 accgtgcggc gaggtgacga tatcggaaat cgcatcatcg ccgatgttgg ttgcctcgcc
      961 cttccacgcg tccggaagac cgtcgctcca atattcgaac cccggattgg caatgacatt
     1021 ggtccccgaa gagccgccat cgggtggcgg ggcatcgata ggggtatccg tcgggctgtc
     1081 gctaaccaca tccggttcgt tgccatcggg caaatccgct tcgggagtgt ccgtcaccac
     1141 cacatcggag aacgtgtcag aggccgtatc cgatgccgta tcggaagagg tttcctcggt
     1201 tccatcgggg ccagtatcca cggtgctatc cgcattcgta tccgagccgg cgtcggccgc
     1261 gacgtcggag ttagcatccg ttagcggtac attgtggtac tcctctgacg aatcactgga
     1321 acaggcagcg acaccgaagg ataggaaaac ggcgcaataa aacactcgac gcatcgtccg
     1381 gcaccttaaa taagtgtggg aaaacgggtg tatagcggaa ggtaggcagt ggcaccagag
     1441 gctgtcggga gaatgaagca aaggttgaat gcggatatgt tggccttgtc tcgcgaattg
     1501 tcgtttcctt tgctcgtgat ggatttgggc cgacgcgttc attttcgtgt tccgaccgcc
     1561 gcccgcgtgg attggcatgc ccggggaacg tgtacgcacc atgtttcgac gacgcgggat
     1621 tggagccggg gaggagtgtt cattcgcacc gacatacccc gagcccccgg ggaattgctc
     1681 cacgttttga tggatgtcgg ggaggttatc gtccagttcg aggcgactgt cgctcatcga
     1741 tgcaaagacg gcatgggtgt tcgtgtggat tcgagttcgg ttcgaatcct gtaatcgtgg
     1801 ctcggggtag ggtatcttat aaattacgcg gaaaacctgc cctggagttg ccatgcgtcg
     1861 attgctgccg gtctgttgtg ccttgtgttg cctcgcccct gcctatgctt tcgcgaatcc
     1921 ttccttcact ggcccggtga cgatgcgcga tggggtagct ctggctaccg acgtgtacct
     1981 tccggagggg cccggaccct ttcccgtgtt ggtcgctcga actccctacg acaaggcggg
     2041 agggcagggg atcggcgatg ggcttcgaga tgcgggagtg gtcacggtcg tgcaagacac
     2101 gcgcgggcgt ttcgcatcgc aaggcgtgga ttgtctgttt caatgcgatg gggaggatgg
     2161 ttttgacacc ttggcttggg tggccgctca accctggtgc aatgggcgtg tggtgacctg
     2221 gggtggctcc gcccttggca tcgtgcagta tatggccgcg gttcatgctc caccggctct
     2281 cgatgccatg tgggtggaag tagcaacccc aaccgtgtac caacatgcgt ttttccaaaa
     2341 tggtgccttt cgacaatcca tgatggaagg ctggcttgcc ggtcaacata gcgacttttt
     2401 cctcgatacc ctggcggaac acccgctcga cgacggcacc tgggattcgg tgcaaaccgc
     2461 cgatcgttac gaccaggtca tcgtgcccgg cgtccactat ggtggatggt acgatatctt
     2521 caaccagggc acgatcgatg cctttgtcgg ctatcaacac cacggtggcc ccggtgccaa
     2581 aggcaaacaa aaactcgtca tgggaccatg gacccatgcc ctcaacacca cgcaagcagg
     2641 agaattgacc tatccatcga atgcagcggg cgtacccggc ggtaccgata cgatgctcgt
     2701 gacctggctg gcgcactatc tggggttcga actaagctcg gcagccgtcg atgccattcc
     2761 caccgtccaa tactacgtca tgggcgatgt gaaagatcct tccgctcccg gcaatacctg
     2821 gcgttcggcg gatgattggc cgattcccgc cgcacccgtg cgaatgcatc tgcatcccgg
     2881 tggcatgctg gacgagtcct gtcccacggt gaacgacgcg cggacgagct acctttacga
     2941 ccccgcgttt cccagtccga ccaaaggggg agccaacttg ctcatccccg ccggccccgt
     3001 ggaccagacc atcgtcgaaa atcgcagcga cgtgatcgtt ttttcttccg cggttctcac
     3061 gaaacctatg gaaattacag gtagaattcg cgcttccctg tgggtcgaaa ccgatgccgt
     3121 cgatacggat atcaacgtcc ggttgactga cgtttatccc gacgggcgat cgatgctcgt
     3181 gctcgatggg gtgcaacgac tcggttatcg aaacggcagt ggctccctcg agcccgttcc
     3241 tatcggtcaa cccatacctg tcaccgtgga cttgtggagc acatccatca tcttggcggc
     3301 cgggcacaag cttcgggtga gcatcacctc atcgaacgcg ccgcgcttct ggccgaatcc
     3361 caacgatggc tcgacatacc gaaacacgaa atcacccacg aaggcgaatg tgtcgatctt
     3421 gcacgatcaa cagcatccct cctacgtgga actgcctgat ccgaatcgtc ccgatacgga
     3481 tatcaccgtt tgcgaccctc acgatgcggg cgcggaagcc tcgaccgatg ggagcgtaac
     3541 gcacgacgct ccccatgccg aggatggcgc catcgagaca ggcgtggacg caagcgttga
     3601 tgctcccttg ctagaagatg cgctcgatac gaaagaaggc ggtagctcgt cagcagccac
     3661 ggcagaaggc gatgggggat gtgggtgtcg cacgaaaacg ggctcttcgg caacgccttg
     3721 gcttgctctt gcctggctct tcctttctgt tgtaaatatg cgaaaatatt caagaaaaca
     3781 tgtattccgc caggccaatc cacacgtgca atcatgagag tccgaaacgc aggcggacgt
     3841 tgcgcccctg accgatccca tcatttttgc cgaactggaa aaggatatgc gctgagaatt
     3901 tctagatctt ggttttggct cggtccttgg gccgccgcca acagttgtct tgagctactt
     3961 aacatcggac tggcacactg cggtgatcaa catgggatga ccgtgatcgt tttgcgtgcg
     4021 ccgtgatcgt gagcgttttt gtgtaccgta cgccgtgatc gtgagcgttt ttgtgtaccg
     4081 tacgccgtga ccgtgatcgt gggccgtgat cgtgaccgtg gaggaaaggc ccgggggaac
     4141 atgaagggat ggctaacctc aaccgacacg gtggaggtcg ttcgaaagcc ccccggacaa
     4201 cgagcgaaac cacgccctca gtggggttgc ttatgcttgt cgagccagtc gcaggcttgc
     4261 tgctgcatca tcttcatgag cacttgggtt tggttgcctg cttctttccc catttcgtcg
     4321 tccgttgtgt tctgaaggag gttcagcatg ccgaaattgc cttggcactt cgcctgctgc
     4381 tcttggattt cacccttttt gacgcattca atgccggccc actcttggtc cgacgcacag
     4441 gcctgaaccg cctcatccct gttgtgggcg gtgatcacgg cagccag
//
LOCUS       DAOSOR010000494         4469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_19540_length_4469_cov_4.261441, whole genome shotgun sequence.
ACCESSION   DAOSOR010000494 DAOSOR010000000
VERSION     DAOSOR010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19540_length_4469_cov_4.261441"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PK710_20325"
     CDS             complement(<1..305)
                     /locus_tag="PK710_20325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12134.1"
                     /translation="MITRRQSLDRMLWAAIGVALPTTGCATCPSPSPFLTTPLPQPQP
                     CQGPAAALREEDVALLRSTTRPLPIVLRDADQTEILRRHARIVSPSDRDHLGDLERRL
                     "
     gene            431..2401
                     /locus_tag="PK710_20330"
     CDS             431..2401
                     /locus_tag="PK710_20330"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOT12135.1"
                     /translation="MPSRWSPVPSWIASVAPLIVALVVAYVDSVWGVVAGIGCGLLLF
                     GCAQVRPTRPIAQTVATLAVVGALGVVGVHAVHRHVLMLLLMVASTLMLGSLWRKVPQ
                     GDRIRIEREPHPLEGTSVASICLVAFVALESRAASAAGVAAIGAFIASIPWMRVLRRQ
                     WNNWLAWCAVTILLVAMGMSAWVAITRVSGAYLMISAVLSGMSALLSMVRLRAGRGIL
                     ALVLEHPARLLVGTFAGLCAVGTVMLALPWSSPRTEGVGILDAAFSSVSASCVTGLTV
                     LDPSTDLSVFGQLSLLLLIQAGGLGIMTISSSALSLLGRRLSLRQEAVMASLVSRENR
                     ADVYHALRRTLSVTLVFETVGAWILAFSFVRLGTPWPKALWHGVFTSISAFCNAGFAL
                     QPDNLIHYQRSPIVLDTVSLLIIAGGLSPVVIVALPHALRGRRLGLQAKVAIVSTVFL
                     LVLGSISYGALEWERTLAGLTTWDRIHNAWFQSVTLRTAGFCSVDYQGLHPATWVMMM
                     VLMFVGGCPGGTAGGIKTTTAFVLLVALLGALRGKASAEVFGRTIPAPTVFKATAIAT
                     LSGMATMIAVSLLLIAQPLAIPVALFEVVSALGTVGLSLGGTAQLDETGKILIMGCMF
                     LGRVGPLTAFLILSDRRAASSWELPAEEIEVG"
     gene            2424..3083
                     /locus_tag="PK710_20335"
     CDS             2424..3083
                     /locus_tag="PK710_20335"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOT12136.1"
                     /translation="MKKQALVLGLGPFGMALVRSLTALGVDVLAVDKNPKLTRLAADI
                     AAEAASFDGADEEALARAAPGRRDVCICAIGDESRDSAIMCTALLRQMGARRVIARAA
                     DPVLARILKLVGAHEVVNPENAFGERLAVHIAHEGVMGEYPLSAGIIITELLVPEAFV
                     GKTLIELELPRKHGITVVALQRGSDTVPTPDPRSPLRRNDMLVVVSRVGAVSTLLDQL
                     E"
     gene            3093..4016
                     /locus_tag="PK710_20340"
     CDS             3093..4016
                     /locus_tag="PK710_20340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12137.1"
                     /translation="MEFKRDMYLGMGALVLSLIIVCVGSVWLFARMSPAIGHVLDEND
                     ESLEAVEQILAVFAQAGPDSVNPSVVDQCNKQLERVRNNITESGEHAEIETIDRAWTR
                     AMAGDASSRKQVVEALMRLASVNRHAMHEANQQAQRLGPRGAWAVTLLCVFGVASGLL
                     VIRKVQQRILVPIDELTQVLNAVHAGDVYRRCTIAGAPQTLTTVMRSINAVLDRIHPS
                     NSEHEKVVEGYRYLADDYPSVLIDDELRVLGVSQAVVAAMDEQAWRRIMQGLGCAMNG
                     EVKSPIQSCESVADRIFLCRIEVDSHSETAS"
     gene            complement(4057..>4469)
                     /locus_tag="PK710_20345"
     CDS             complement(4057..>4469)
                     /locus_tag="PK710_20345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT12138.1"
                     /translation="REDVLPTAHHLLKRLKPDGDPRLSAQVASALTRYDWPGNVRELA
                     NVLERSLLFAGNGEIRPEHLPPSLRALCSQQDAEPITETGVRPGVPLEEVERELIQKT
                     LMALGGNRTRTAEALGLSRRALLYKIKRFGLRDG"
BASE COUNT          827 a         1128 c         1461 g         1053 t
ORIGIN      
        1 agtcgccgtt cgagatcccc gaggtggtcc cgatcggatg gtgagacgat ccgcgcatgg
       61 cggcgaagga tttcggtttg gtcagcgtct ctaagaacga tgggtagcgg cctggtggtg
      121 gaacgaagaa gggcgacatc ttcttcgcgc aaagcggcgg cgggtccttg gcatggttgc
      181 ggctggggca ggggggtggt gagaaagggg gaaggagacg gacaagtcgc gcagccggtc
      241 gtgggtaggg ccacaccgat cgcggcccac agcatcctat cgaggctttg gcgtctggtg
      301 atcatggcga aaacactgta gcgaagaatc ggagttcggg cgaggctggg cgttcgtagc
      361 gataggccca gcatattcat tccgctttgt gctcagttgt gatagcggtt ccgggcagag
      421 gaattggcgc atgccatccc gttggtcacc tgttccgtcg tggatcgcaa gcgtggctcc
      481 gctgattgtc gcgctcgtcg tggcttacgt ggatagcgta tggggggttg ttgcgggaat
      541 aggctgtggt ttgctgttat ttgggtgcgc gcaggtgcgc cccactcgcc cgattgcgca
      601 gacggttgcc accttggccg tggtgggagc gcttggtgtc gtcggggtgc atgctgttca
      661 tcgacacgtt ctgatgctgc tgttgatggt tgcgtcaacc ttgatgcttg gttccttgtg
      721 gagaaaggta ccgcagggag accgtatacg gatagaacgt gaacctcatc ccctcgaagg
      781 gacgagtgta gcttcgatct gtctcgttgc attcgtggcg ttggaatcaa gggccgcgag
      841 cgccgcaggg gtcgccgcga tcggggcttt catcgcgtcg ataccgtgga tgcgagtgtt
      901 gcgaaggcaa tggaacaact ggcttgcttg gtgcgcggtg acgattcttc tcgtggcaat
      961 ggggatgtcg gcatgggtag cgataacgcg tgtgtcggga gcctatctca tgatttccgc
     1021 cgtgttgtcg ggtatgagcg cgctattatc catggttcgt ttgcgcgcag gaaggggaat
     1081 tctcgcgctc gtgctggaac acccggcaag attgctggtg ggtacgtttg ccggtttatg
     1141 cgccgttgga accgtgatgc ttgctttgcc ctggagttca ccgcggaccg aaggggtggg
     1201 tatccttgac gctgcatttt catccgtgag cgcgtcgtgc gttacgggac tgacggtgtt
     1261 ggatccgtcg acggatctat ccgttttcgg acagctatcc ttgctcctgt tgattcaggc
     1321 cggtggattg ggaatcatga cgatctccag ttccgccctg tctttgctgg ggcgacgatt
     1381 atcgcttcgg caagaggcgg tgatggcctc gctggtgagc cgcgaaaacc gagcggacgt
     1441 ataccacgcg ttgcgtcgca cgctttccgt gacgttggtg ttcgagaccg tgggtgcgtg
     1501 gatattggcg ttttctttcg tgaggctcgg aaccccttgg ccgaaagccc tgtggcatgg
     1561 cgtgttcact tcgatttctg ccttttgtaa tgctggcttt gcgttgcaac cagataacct
     1621 catccattat cagcgaagcc ccatcgtgct ggataccgtg agcctgctca tcattgcggg
     1681 tgggttgtct cccgtggtga ttgtggctct tccccacgcc ttgcgcggtc gacggcttgg
     1741 attacaggcg aaagtggcca tcgtctcgac ggtatttctc cttgtattgg gctcgatttc
     1801 gtatggggcc ctcgaatggg aacggaccct cgcagggctc acgacctggg atcgaattca
     1861 caacgcctgg tttcaatccg taacgcttcg gacggcgggg ttttgctcgg tggattatca
     1921 gggactgcat ccggccacgt gggtgatgat gatggtgctg atgttcgtcg gaggctgtcc
     1981 aggaggcacc gccggtggca tcaaaaccac gactgccttt gtgttgttgg tggcattgtt
     2041 gggcgcgttg cgggggaagg catcggcgga ggtttttggc aggacgatcc ctgctcccac
     2101 cgtattcaag gcgacggcca ttgctacgct cagtgggatg gcgacgatga ttgcggtgag
     2161 tctgttgctc atcgcgcagc ctttggcaat accggtggct ttgttcgagg tagtatctgc
     2221 tttgggtacg gtcgggttga gccttggcgg cacggcgcaa ctcgatgaga caggaaaaat
     2281 cctcatcatg gggtgcatgt ttttggggcg tgtcggtccg ctcaccgcgt ttttgattct
     2341 ctcggatcga cgggcggctt cgtcgtggga attgccagcg gaagagatcg aagtgggatg
     2401 agtggcggga aaggcatgac gagatgaaaa agcaggcatt ggtgctcggt ctcggcccgt
     2461 ttgggatggc tttggtgcgc tcgttgacag ctcttggtgt ggatgtgttg gcggtggata
     2521 aaaaccccaa actgacccga ttggcagccg atattgcggc ggaagcagct tccttcgatg
     2581 gtgcggacga agaggcgctg gcacgcgcgg ccccggggcg acgagacgtg tgtatttgcg
     2641 caatcggtga tgaatcaagg gattctgcga ttatgtgtac ggcgttgcta cggcagatgg
     2701 gggctcgacg cgtgattgca agagcggcag atcccgtcct cgctcgtatt ctcaaactgg
     2761 tcggggccca tgaggtggtg aatccggaaa acgcttttgg ggagcgcctg gctgtacata
     2821 tcgcccatga aggtgtcatg ggagagtatc ccctcagcgc tggcatcatc atcacggaat
     2881 tgctcgttcc tgaggcgttt gtgggaaaaa ccttgatcga gctggaactg ccacgcaagc
     2941 atggcatcac cgttgtggcc ctccaacgtg gctctgacac cgttccaacc cccgaccccc
     3001 gatctccatt gcgccgcaat gacatgctgg tggtggtgtc gagagtgggg gcggtatcca
     3061 cgctcctcga tcaactggag taggggcaag ttatggaatt caaacgagat atgtacctcg
     3121 ggatgggcgc tctcgtgttg tccctcatca tcgtttgtgt cggttccgtt tggctattcg
     3181 cacgaatgag ccccgcgatc gggcatgtac ttgatgaaaa cgatgagtcc ctcgaagcgg
     3241 tcgagcaaat tctcgcagtt ttcgcccaag ccggtccaga ttcggtgaac ccttccgtcg
     3301 tggaccaatg caacaagcaa ctcgagcgtg ttcgaaacaa catcacggag tccggcgaac
     3361 acgccgagat cgaaacgatc gacagggcgt ggactcgagc gatggctggc gatgcttcgt
     3421 cgcgaaaaca ggttgtggaa gccttgatga gattggcttc ggtcaatcgg cacgcgatgc
     3481 acgaggcgaa tcaacaggcc caacggcttg gccccagagg ggcttgggcc gtaaccctgc
     3541 tgtgtgtgtt cggggtggcg agcggccttc tcgtcatccg aaaagtgcag cagcggattt
     3601 tggttcccat cgacgagctt actcaggttc tgaacgcggt gcatgcagga gatgtgtacc
     3661 gacgatgcac gatcgcgggc gctccccaga cattgaccac ggtcatgcga tcaatcaatg
     3721 cagttctcga ccgcatccat ccgtcgaact cggagcatga aaaagtggtt gaggggtatc
     3781 gttacctggc cgacgactat cccagcgtgc tgattgacga cgaacttcgt gtgctcgggg
     3841 taagccaagc ggtggttgcc gccatggacg aacaagcttg gaggaggatt atgcaggggc
     3901 ttggttgtgc gatgaacggg gaagtgaaat caccgattca atcgtgtgaa agcgtggccg
     3961 acaggatctt tctttgccgc atcgaggtcg acagccattc cgaaacggcg tcttgaatgg
     4021 taggcgcgcg gaggaacgag ttggtgattg gatacgttat ccatcccgca acccaaatcg
     4081 cttgatctta tatagcagtg ctcgtcgtga cagcccaaga gcctcagcgg tgcgtgttcg
     4141 attgccgcca agggccatca acgttttttg gatcaattcg cgctcgactt cttccaaagg
     4201 aactcctgga cgcacgccgg tttcggtgat cggctcggca tcttgctgcg aacacaacgc
     4261 gcgtaaagag ggaggcaagt gttcggggcg aatctcgcca ttacccgcaa acaataacga
     4321 gcgctctaac acattcgcca gttcccgcac gttgcccggc caatcatacc tcgtcaacgc
     4381 gctcgccact tgagcggata atcgaggatc gccgtcgggt ttgaggcgtt tcaacaagtg
     4441 gtgcgcggtg ggaagcacat cttcccttc
//
LOCUS       DAOSOR010000495         4461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_19584_length_4461_cov_4.496823, whole genome shotgun sequence.
ACCESSION   DAOSOR010000495 DAOSOR010000000
VERSION     DAOSOR010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19584_length_4461_cov_4.496823"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PK710_20350"
     CDS             complement(<1..258)
                     /locus_tag="PK710_20350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12139.1"
                     /translation="MSLIACSNDGDGDSHGANGTGGSSGTGGSSGTGGSSATGGSSGT
                     GGTSATGGAAGTAGGPADDSCASVRLTSYTASAKGWCEYDRT"
     gene            461..1069
                     /locus_tag="PK710_20355"
     CDS             461..1069
                     /locus_tag="PK710_20355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12140.1"
                     /translation="MRDWDLIKITFTILPMVLVAAGCRCSNTPSEQVLPTSKASASPL
                     PTVGSAQSCPARPAWAGEPTPDMEIELLHARTVGTKEQGTGYRLYRDGSLETFDTVTV
                     EPDDQGRMVLKTVAGSWKKRGKVKESARLSLKKKIASQDPMTLRGKWRTTGSTTTRTH
                     LLTRVDEQENVLCYVGEDAPSSIQELQKSIHDLMGQLETATP"
     gene            1193..1771
                     /locus_tag="PK710_20360"
     CDS             1193..1771
                     /locus_tag="PK710_20360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12141.1"
                     /translation="MSYAAPDELRRVERAVADQILQFPVGPCFITQDWQNPAKPRLHC
                     LVATRRLQDNKFLPTMFLLDFGCQGVLDAYFAEPMGNETLSALLDKLHPVFPQGFESI
                     DPSIASRIVGACIQYATKLGFELKPELFSFLSALSEHIDTTIDIEFGRGGKPLYCPNP
                     NEDTRPIVKRLVEAVGPDGFNVELPHTRGTSP"
     gene            1768..2205
                     /locus_tag="PK710_20365"
     CDS             1768..2205
                     /locus_tag="PK710_20365"
                     /inference="COORDINATES: protein motif:HMM:NF013089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HOT12142.1"
                     /translation="MTHVIALLAALALNATANLLIKFGMRGIDLELDGASALDGGLLG
                     LIKLLLRHWQVLAGLACFALNVVFYAFALQKLPISVAYPIMVTTGFAIIVTVAGWKLH
                     ESLTVYQWIGVVTILIGVALVAKDAGRQMGGGKTGEAHQVHDG"
     gene            2298..3134
                     /locus_tag="PK710_20370"
     CDS             2298..3134
                     /locus_tag="PK710_20370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12143.1"
                     /translation="MGSQRFSERAEEARRAQWRLNDLPWDERIRLAGDSRRERLLAKL
                     DLLGACEALYHLKLGARSRAGTLYFHTWKQDDPIIECLEWLDTDELRHLRTLRRLIQT
                     VHSSEQEDIEAKPHADPTRMWRVGRNPRGGPSSLEAEVLRALVDEATTRCLLSTISRQ
                     SRVPIVRAAFSSCARDDGRHIAFLSDLAKLSLNHDDTMHVVRMQGVVLRHIARIQSSL
                     RPYFRVFAAVTDATIDNVASEVFRAVSAVVADLGTPWVRYPTARVIHEADRSPWMLWL
                     LR"
     gene            complement(3188..4366)
                     /locus_tag="PK710_20375"
     CDS             complement(3188..4366)
                     /locus_tag="PK710_20375"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOT12144.1"
                     /translation="MRIRPSNRAGWDLTPNAFSAACQRRIREGQQLLDLVQSNPTRIG
                     LDTLTPVMLDALRHPASTRYEPNPLGIPCAREAVASWYARRGLAVNPDRLMLTASSSE
                     AYGTLLKLLCDPGDSILVPAPSYPLFDQLARLEGVTVKRYPLMMEDGWRVCEAMVQQV
                     VDSRTRAIFAVSPNNPTGSYLHSSELRSIERVAREHNLAVVCDEVFAEYVWKEDTDRV
                     VCAALEADVPTFSLGGLSKSACLPQMKLGWIVMGEHVDQDTIHRLEMIADTYLSVSTP
                     IQHAAGAFLDGATLARDMLAQRIQENLAVLEHSIRATPVSVLPIDGGWYACLRVPSVM
                     SDEQWAELLVLEDGVVVHPGSFFGFPIPGILVIGLIAATNVFREGIQRLVARGEKVIA
                     "
BASE COUNT         1019 a         1308 c         1210 g          924 t
ORIGIN      
        1 cgttcgatcg tattcgcacc agcctttcgc ggaagccgta tacgacgtga gccgcacgct
       61 agcgcaagag tcatcggcag ggcctcctgc cgtaccagcg gcgcctccgg ttgcgctcgt
      121 tcctcccgtt ccgctcgatc ctcccgttgc gctcgagcct cctgttccgc tcgagcctcc
      181 tgttccgctc gagcctcccg ttccattggc accgtgcgaa tcgccatcgc catcgttgct
      241 gcatgcgatg agactcacaa cgcataacgt tgaaaacata tatgtgcgca cggagcgatt
      301 ttagcattcg aagccatgtg cttcgacaag caagttcgac gagcatgcgc gtggccggtc
      361 aattcgcata aggcaagggc atgcgccccc gcggcaagtt cttgctcccc cctcgttcct
      421 ctgtcatttt gccgccgctg attcgaccgt ggagatagtc gtgcgtgatt gggatttgat
      481 caagattacg ttcaccatcc ttccgatggt tttggtggcg gcgggatgtc gctgtagtaa
      541 taccccctcg gaacaagtgc ttccaacttc gaaagccagc gcgtcacccc tacccacggt
      601 cggttcggcc caatcctgtc cagcgcgtcc cgcctgggct ggcgaaccaa ccccagatat
      661 ggaaatcgag cttcttcatg cgcgcacggt aggcaccaag gagcaaggca cggggtatcg
      721 tctttatcga gatgggagtc tcgaaacctt cgatacggtc acggtcgagc ccgatgacca
      781 aggcagaatg gtgctcaaga ccgtggccgg ttcttggaag aagcggggaa aagtcaaaga
      841 atccgcacgg ttatccttga agaaaaaaat cgcttctcag gatcccatga cccttcgcgg
      901 caagtggcga acgacaggaa gcacaacaac ccgaacgcac ttgcttaccc gagtggatga
      961 acaggaaaac gtgctttgct atgtcggcga agacgcccca tcgtcgatac aggagcttca
     1021 gaaaagcatt catgatctga tggggcagtt ggaaaccgca acaccatgac aggggatgca
     1081 agacgatacg gggcgaacag caaacgcctt ggagacgaaa gaaatgcctg accatcgaca
     1141 gaagaggctt gccaagcaga aaaagaagcg tgcccatgta agtcgtgcag ccatgtccta
     1201 cgccgccccg gatgagcttc gacgggtcga acgagcggtt gccgatcaga tactccagtt
     1261 tcccgtgggc ccttgtttca tcacgcaaga ctggcaaaac cctgccaagc cacgtctgca
     1321 ctgcctcgtg gccacacgtc gcttgcaaga caacaagttc cttccgacca tgttcttact
     1381 ggatttcggc tgtcaggggg ttcttgacgc gtacttcgca gaacccatgg gaaatgagac
     1441 cttgagcgcg ttgctcgaca agctgcatcc tgtgtttccg cagggattcg agtcgatcga
     1501 ccccagcatc gcctcccgta tcgtcggcgc ttgcatacaa tacgcgacaa agctcgggtt
     1561 cgaattgaaa ccggagcttt tctccttcct gtccgcgttg tcggaacata tcgacaccac
     1621 gatcgacatc gaattcggtc gcggcgggaa accgctgtat tgcccgaatc cgaatgaaga
     1681 tacacggccg atcgtcaaac gtctcgtcga agcggtcggc cccgatgggt tcaacgtcga
     1741 gctgccacac acaagaggca caagcccatg acacacgtta tcgcgctcct tgcagcgctg
     1801 gctttgaatg ccacggccaa cctgctcatc aagttcggca tgcgcggcat cgatctggaa
     1861 ctcgatggcg ccagtgctct cgacggtgga ttgttggggc tgatcaagtt gctgcttcgg
     1921 cattggcagg tgctggcagg gcttgcctgt ttcgccctca atgtggtttt ctacgcgttt
     1981 gcactgcaga aactaccgat cagcgttgcc tatcccatca tggtgacaac gggtttcgcc
     2041 atcatcgtca ccgtcgcagg atggaagctg catgaaagct tgacggtcta ccaatggatt
     2101 ggtgtcgtaa ccattctgat aggcgtggca ttggtggcaa aagatgccgg ccgtcagatg
     2161 ggtgggggaa aaacaggcga ggcacatcag gttcacgatg ggtaaacccc gattcccatg
     2221 ccatcgttcc catccgcaag gggagcgaaa atattgtaac cctaccccga cagaattaag
     2281 gcatccttcc agccgccatg ggtagtcaac gattctcgga gcgagcggaa gaagcacgaa
     2341 gagcccaatg gagactcaac gacttgccgt gggacgagag gatcaggctc gcgggagata
     2401 gccgtcgcga gcgtttgctc gccaaactcg atcttctcgg ggcttgcgaa gctttgtatc
     2461 acctgaagct cggagcccga tcgcgtgcag gaacgttgta ttttcatacc tggaagcagg
     2521 acgaccccat catcgaatgt ctggaatggt tggacacgga tgaattgcgg catcttcgaa
     2581 cgctgcgacg gctcatccag accgtccatt cgtccgagca agaggatatc gaagccaaac
     2641 cacacgctga tccaacacga atgtggcggg tagggcgcaa tccccgcggc ggaccctcga
     2701 gcctcgaagc ggaagtgttg cgcgctctcg tagatgaggc cacgacgcga tgcttgttga
     2761 gcaccatatc tcgtcaatca cgcgttccga tcgtgcgagc tgccttttct tcctgcgccc
     2821 gggacgacgg tcgtcatatc gcttttttat ccgacctggc gaagttgtct ttgaaccacg
     2881 acgacaccat gcacgtggtg cgaatgcaag gggtcgtact ccgacacatc gctcgcatac
     2941 aaagctcgct tcgaccgtat tttcgcgtgt ttgccgcggt cacggacgcc acgatcgaca
     3001 acgtggcaag cgaagtcttt cgcgcggttt ctgctgtcgt ggcggatctc ggcacaccat
     3061 gggttcgata cccaaccgct cgtgtcatcc atgaggccga tcgctcccct tggatgcttt
     3121 ggctccttcg atagtgatta gcctccccct tcgagcgaac ccaaggagga aaacgtcgac
     3181 ggccccgtca cgcaatcacc ttctccccac gtgccacaag cctttgaatt ccttcacgaa
     3241 atacattagt cgccgcgatc aatccaatca cgagtattcc tggaatggga aaaccaaaaa
     3301 acgatccggg atgaacaacg accccatcct ccaacacaag caattccgcc cactgttcat
     3361 cggacatcac cgacggaacg cgaagacaag cgtaccaccc accatcgatg ggcaacacgg
     3421 acaccggtgt cgcgcgaatc gaatgttcga gaacggcaag gttttcttgg atgcgctgag
     3481 ccaacatgtc acgagccaac gttgccccat cgaggaaggc tcccgcggcg tgttgaatcg
     3541 gtgtcgatac ggaaagatag gtatccgcga tcatttccaa tcggtgaatc gtgtcttgat
     3601 ccacatgctc gcccatgacg atccagccca gcttcatctg cggcaaacaa gccgacttcg
     3661 ataagccccc aaggctgaat gtcggaacat cggcttccaa agccgcacaa acgactcggt
     3721 ccgtatcctc tttccaaaca tactccgcga atacctcgtc gcacaccacc gccaaattat
     3781 gctctcgcgc cactcgctct atcgaacgca gctcgctgct gtgaagatac gaacccgtgg
     3841 gattgttggg actgaccgcg aaaatagcgc gtgtacggga atcgacgact tgttgcacca
     3901 tcgcttcgca aacacgccag ccgtcttcca tcatcaatgg atagcgtttg acagtcacac
     3961 cctcgagccg agcaagttga tcgaataggg gatagcttgg tgcgggaacg aggatcgaat
     4021 cgccgggatc gcataatagc ttgagcaacg tgccataggc ttcggacgag gacgccgtga
     4081 gcatcaatcg atccggattt acggcaagtc cccgcctcgc gtaccacgag gctacggctt
     4141 cacgagcaca cggaatcccc aggggattcg gctcataccg tgtggatgca gggtgacgca
     4201 aagcatcgag catcacaggc gtgagtgtat cgagaccaat gcgagttggg ttggattgaa
     4261 ccagatccaa tagttgctga ccttcgcgta tcctacgttg gcaagccgcg gaaaaggcgt
     4321 tcggggtcag gtcccacccc gctcgattcg agggtcgaat cctcatgatc gtttcatgat
     4381 acccgcgagg ttgcggtgtc aatttccaac gagccgcaaa tgccgaggag gaatggaatc
     4441 atcatagggc accgggtaat g
//
LOCUS       DAOSOR010000496         4428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_19767_length_4428_cov_5.161217, whole genome shotgun sequence.
ACCESSION   DAOSOR010000496 DAOSOR010000000
VERSION     DAOSOR010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19767_length_4428_cov_5.161217"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..1499)
                     /locus_tag="PK710_20380"
     CDS             complement(207..1499)
                     /locus_tag="PK710_20380"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HOT12145.1"
                     /translation="MPVHEARIPGFHKHSLTERHRILSENTSIPIDEITRALHGGGID
                     PETADKIVENVVGTYALPFGLALNFLINGTPYIVPMVVEEPSVIAAASNAARIVREGG
                     GFSATVDPSIMACQIQTYDVPNIPIAVERVLAAKRMLLEHAAKSVEPLVALGGGPTDL
                     EVRVLAADMIVVHVYTDCLDAMGANTVNTIAEALGPEIARLTEGRLGLRILSNLCDRR
                     LVRARCRIPAAAVAVSGQEQQTVDGIVNASRFAELDPYRAATHNKGIMNGIDPVVIAT
                     GNDWRAVEAGAHAYAARSGHYAPLAVWRKTQTDEGTMLEGSLEMPLSLGIVGGTLRVH
                     PAARLALKIMDISTAGQLAQVAACAGLASNLAALRALATEGIQRGHMSLHARSVALQA
                     GAIDDEVQQVANRISEDRLITVEHAQTVLQELRKPSSS"
     gene            2101..2313
                     /locus_tag="PK710_20385"
     CDS             2101..2313
                     /locus_tag="PK710_20385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12146.1"
                     /translation="MSFWASLFGKKVHCHGCGVVLPSHVKAKDGETYCSDVCRDNYRR
                     LSTMPPTPPTQDAPGQEASPSDSLVR"
     gene            complement(2317..3402)
                     /gene="cheB"
                     /locus_tag="PK710_20390"
     CDS             complement(2317..3402)
                     /gene="cheB"
                     /locus_tag="PK710_20390"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HOT12147.1"
                     /translation="MASIRVLVVDDSLLTVQVMTRVLSSDPQIEVVGRALRGDRAIEE
                     AERLRPDVITMDVHMPGMDGIEATRQIVKRFGIPIVIVSAFATASSVAAGTLQALQNG
                     AVDCVQKPSGEIGLDMERVGAELKSKVRAAALAKPASLTSLADRSVTRSDTAMPGGTS
                     TVRAQPWDRVKPSEVSLVAMGISTGGPSTLEQFVPQISGDLPVPIVMVIHMSAPFIPI
                     LAQRLDGASAIGVRVASAGEELRSRTLYLAPADVHLEVTRDLRVRLVAGRPVWGCIPA
                     VDVLFESVARNVGPRAIGMVLTGMGRDGAKGLRAMADSGATTAAQDEATSVVYGMPRA
                     AMQAGAAQYEVPLDRIAAFVRQAIRGT"
     gene            complement(3415..>4428)
                     /locus_tag="PK710_20395"
     CDS             complement(3415..>4428)
                     /locus_tag="PK710_20395"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOT12148.1"
                     /translation="DSRIVRNLVRGGLVKQGFEVETAMDGLEALDKLEAMVAMPSMII
                     SDFEMPRMNGFELGMELRNRPQTKGIPLIVMSARDDKRYKSLMKTAGAVGYIGKPFEP
                     DKVVALVERVLAEQRLKAEKARIRELVADDAMAQTVREIRLGKRSVAKSESMTLLFSD
                     LKGFSTMCTRLSAETVVELLNGYFDAMIPAIQRNQGDVDKFIGDAIMARFRDPEEEVR
                     DPSPLRAIRAALDMQQALREYNRTQPGDQQLHMRIGINHGQVVIGSIGAKSRRDFTTI
                     GDEVNRAQRLESNARVDGICIAENTYQLVRDRVIVEPRESLIQLKGIDQPVQVYDVLG
                     IRP"
BASE COUNT          983 a         1372 c         1190 g          883 t
ORIGIN      
        1 ccctcctggg attgagcgat accacgttgg cacgacgaac ggatcgtggt caacccatgg
       61 cgagaaacat cggggaggaa cgcagaaggt caggacggtt gggtgatgag gaggaaacga
      121 acagaagcta tccagatacg gacaatttgt accaacacaa ctagttgaat ttcaacacaa
      181 atatcgcaga ccacagcgga aacagatcat gaggaagacg gttttcgaag ctcctgcagc
      241 acggtttggg cgtgctcgac cgtgatgagg cggtcctcgg aaattcggtt ggccacctgc
      301 tgcacttcgt catcgatggc tcctgcttgc agggcgacgg atcgagcgtg caacgacata
      361 tgccctcgct gaatcccttc ggtggcgaga gcccgcaacg ccgccaggtt ggaagcaagt
      421 cccgcgcaag cggctacctg ggcgagttgg cccgccgttg atatatccat gattttcagg
      481 gcgagacgcg cggcgggatg gactcgcaag gtgccgccga cgatcccgag gctcaagggc
      541 atctcgaggc tgccttcgag catggttccc tcgtcggtct gggtctttcg ccacaccgcc
      601 agcggggcgt aatggccgga tcgggcggca taggcgtgcg cgccagcttc gacggcccgc
      661 caatcattgc ccgtggcgat cacgaccggg tcgatcccgt tcatgattcc cttgttatgc
      721 gtggcggcgc gataaggatc caactccgcg aaacgtgagg cgttgacgat gccatccacc
      781 gtctgctgct cctgtcccga aacggctacc gcggcggccg gaattcgaca acgagcccga
      841 acgagccgac gatcgcaaag gttggataaa atgcgcaaac caagacgtcc ctcggtcaat
      901 cgggcaatct cggggcccaa tgcctccgcg atggtgttca ccgtattggc ccccatcgca
      961 tcgaggcaat ccgtatacac gtgaacgacg atcatatccg cggctaatac gcggacttcg
     1021 agatccgtgg gtccaccgcc cagagccacg agcggttcga cggacttggc ggcgtgttcg
     1081 agcagcattc gtttggcagc cagcacgcgt tcgacggcga tggggatatt gggaacgtca
     1141 taggtttgga tttggcaggc catgatcgaa ggatcgaccg tggcggaaaa gcctccccct
     1201 tcacgcacga tacgggcggc attgctggcc gcggcgatca cgctgggttc ttccacgacc
     1261 atgggaacga tatagggggt cccattgatc aaaaaattga gtgcgagccc gaaggggagg
     1321 gcgtaggtgc ccacgacatt ttcgacgatt ttgtcggcgg tttcggggtc gatgccgccg
     1381 ccatgcaggg cgcgtgtgat ttcatcgatg gggatgctgg tattttcgct gaggatgcga
     1441 tgccgttcgg tcagggagtg tttgtgaaaa ccggggattc gcgcttcgtg gacgggcatg
     1501 ggtgggaatc taactccaaa tcaagggagg gccgagaggg aagcgcccga gactgcatag
     1561 gaccagcgaa atagctcgag aatcaacgga agaggagctt tggggatggg aagagcaacg
     1621 aatcaaaccc atgagctgat cgagtaaacc ggatcgcgtc cacccatgcg tcgatttcga
     1681 taatatacgt atatgttgtt ccaccaaggg atcacagttg ataatctcat gttaactatg
     1741 ggaatataaa caggaattgt cattttctgc tatctagaat ccgatcctta ctatccgtat
     1801 cagtatattt atatgtttac ggatcatcgt aggacgccta cgaatggagt tccgcacgcc
     1861 gcagcttcct gccatccctt ggaagctgca aagcttgaaa caatgggcaa gcgaagagcc
     1921 attccattcc tatgtggagg ttcaaagccc atcgaccctt ccgtccccct tcgaaagatc
     1981 aagcggccac cgatgggatt ggccctccca aacaaccagg gctagactcc atgagcatcc
     2041 cattgcaacg aatcgggcaa cgttggcggc gtgagcgccg ctctaccatg gaggggaaag
     2101 atgagctttt gggcaagtct gttcggaaag aaagtgcatt gtcacggttg cggagtcgtt
     2161 ttacccagcc acgtcaaagc caaggatggc gaaacgtatt gcagcgatgt ctgccgggat
     2221 aattatcgtc ggctatccac catgcccccc accccaccca cgcaagacgc accgggccag
     2281 gaggcttctc caagcgattc gctcgttcgc tgagcttcac gtccctcgaa tcgcctgacg
     2341 aacaaaagcc gcaatgcgat cgagcggcac ttcatactgc gcggctccag cctgcatcgc
     2401 cgctcgcggc attccataca ccacgctcgt tgcttcatcc tgcgcggccg tcgtcgcacc
     2461 actgtccgcc atcgcacgca aacccttggc accatcacgc cccatacccg tgagcaccat
     2521 ccctatcgca cgaggaccga cattgcgcgc aaccgattcg aacaacacat ccaccgcggg
     2581 aatgcatccc caaacgggtc ggccggccac gagccgaaca cgaaggtctc gcgtcacttc
     2641 gagatgcaca tccgcgggcg caagatacaa ggtccgtgag cgcaattctt cccccgccga
     2701 tgccacgcgc acgccgatcg cactcgcacc atcgagccgc tgcgcaagaa tcggaatgaa
     2761 gggagccgac atgtgaatga ccatcacgat cggtacaggt aagtccccgc tgatctgagg
     2821 aacgaattgc tccaatgtcg aaggtccacc cgtggaaata cccatcgcca cgagactaac
     2881 ttccgaaggt ttcacacgat cccacggctg cgcacgcacg gtagacgttc cgcctggcat
     2941 cgcggtatcc gaccgcgtca ccgagcgatc cgccagcgaa gtcaacgatg cgggttttgc
     3001 caacgccgcg gctcgtacct tcgacttcaa ctccgccccc actcgttcca tgtcgagccc
     3061 aatttcgccc gatggtttct gaacgcaatc caccgcaccg ttttgcagcg cctgcagggt
     3121 tcccgcggcc acactcgatg cggtggcaaa cgcgctcacg atcacaatcg ggatcccaaa
     3181 ccgcttgacg atttgccttg tcgcctcgat gccatccatg cccggcatgt gaacgtccat
     3241 cgtgatgaca tccggccgca atcgctccgc ttcttcaatc gctcgatcac ctcgaagcgc
     3301 acgacccacc acctcgatct gcggatcgct cgacaaaacc cgtgtcatga cctgcacggt
     3361 gagcaatgaa tcgtcgacca cgagaacacg aatcgatgcc acgccccgtt cctcctacgg
     3421 ccgaataccc agcacgtcat acacttgcac gggttgatcg atgcctttta gctgaatcaa
     3481 cgactcacgg ggttcgacga tcacccggtc tcgcacgagt tgatacgtgt tttccgcgat
     3541 gcatattcca tcgacgcgag cattcgattc gagacgctgc gcgcggttga cttcatcacc
     3601 aatcgtagtg aagtcacggc gacttttcgc accgatggat ccgatgacca cctgaccatg
     3661 gttgatcccg atacgcatgt ggagctgttg atcccccggt tgcgtgcgat tgtactcgcg
     3721 aagcgcttgc tgcatgtcca atgccgcacg aatggcgcgc aacgggctcg gatcacgcac
     3781 ttcctcctcc ggatcgcgaa aacgcgccat gatggcgtcg ccaatgaact tatccacatc
     3841 cccctggttg cgctgaatcg ccggaatcat cgcatcgaaa tacccattga gtagctcgac
     3901 caccgtctcc gcgctcaacc gcgtacacat cgtggaaaag cctttgagat cggaaaacag
     3961 caacgtcatc gattcggact tcgccacgct ccgcttgcca aggcgtatct ctcgcacggt
     4021 ttgcgccatg gcatcatcgg ccacaagctc gcgaatcctt gctttttcgg cttttagacg
     4081 ctgttccgcc aacacccgct ccaccaatgc caccacttta tccggctcga aaggcttccc
     4141 gatatagccc accgctcccg ccgtcttcat caaactcttg tagcgcttgt catccctcgc
     4201 gctcatcacg atcagcggaa tccctttcgt ttgcggacgg ttacgcaact ccatgcccaa
     4261 ttcgaaacca ttcatccgag gcatctcgaa gtccgaaatg atcatcgagg gcatcgccac
     4321 catcgcctcg agcttgtcca acgcctcgag gccatccatc gccgtctcta cctcgaaccc
     4381 ttgcttgaca agcccaccac gcacgagatt acgcacgatg cgagaatc
//
LOCUS       DAOSOR010000497         4407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_19891_length_4407_cov_3.455193, whole genome shotgun sequence.
ACCESSION   DAOSOR010000497 DAOSOR010000000
VERSION     DAOSOR010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19891_length_4407_cov_3.455193"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PK710_20400"
     CDS             <1..611
                     /locus_tag="PK710_20400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12149.1"
                     /translation="TFSGKITTFLAKYDVDSALLWSHAFPSASFAVLYGVTAGPNDRV
                     AVTGRVEGSVDWGGGPLSVKGQADCAVAVYDATGKHEASVLIGEKGDAKGDDIRFTSD
                     AILIVGAHSGPLSFRGSSLLEASDQNAFVLALSPDLSSVPWAFSLGAEGEDQALGLDT
                     RGKYVLVAGEFKNSLKYKQLSATSQDVGQAFLLQFLPPPITN"
     gene            complement(749..2083)
                     /locus_tag="PK710_20405"
     CDS             complement(749..2083)
                     /locus_tag="PK710_20405"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOT12150.1"
                     /translation="MNHSEFDIRLGFSYSLGVYLAINALPDALLVMDGMDCTVQMASQ
                     IQGNHDWQSNLISADGAHRILTTHAMPEEVWQNREKAISDVVSYALGRSDSRVIFLGA
                     IRMASITEPPYELILDACAEQTGTEKPLILLPTRSLVDDWLTGYDNTLEYLAKYLPLE
                     PRQGANQGRVAIVGYLMDRNEEDHFGNLRELERLIEGIGGTLVCTWLSGKPTTHLAKI
                     GQADVVISLPYARNAAKILANRTGAALVETELPIGLEGTRTWLETVARALGTQRAAEA
                     FVHKELSQAATKLEAVVSLVSRDMSLALMGEPYLVRGLLGLARELGIPVPLRAIVSAV
                     QNDGQDWLQPDPLDDPITIVDPKEGQLQEPLRVAIEKRGVNLVVATSYVLQDLVPPVA
                     AFELGFPSFHSHALTDQPFMGFRGCLSLVARWVERQAWHHLLRDLRPIPSNG"
     gene            complement(2080..3936)
                     /locus_tag="PK710_20410"
     CDS             complement(2080..3936)
                     /locus_tag="PK710_20410"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOT12151.1"
                     /translation="MTSDLSRIPRYLVAGPDAVAAFHHVFGISPTLRANGTYVLSSIT
                     WKEGMLRVCLTGRLFGRAMIHLQPTHTPGQEKLTVKAESTDAPSHPQVERLVDFLHNR
                     LKATPIEDLLDWLRRDPKTEAMSGVLSEGLLGGLRAGANLAPKPYAHTWTGKPGEVDQ
                     WGLFFDDLVFVFTALSRVNFVLPTTHVVHGCHECTCVTPKIRTRSFFLFYHPRRPHRP
                     AADWLRARLGLGPQYLDQPFRRISTNMNEVDTVRGAEDKLMQAVALAATGQGERIVAI
                     NATCLPKVMGEDVGPAISLARNTPKVHCLSQALPLTHDTSLYTDELEFGQDEPQVMRD
                     LLLPPQGRCLEQEPDGKRIDLVGFPDGPDTLELVTLLERCGAVVNALLLPDIDVAVCR
                     RTPKPNLQVRFPNPNYTRLYHEVFDAMADQIVTPPAPYGIDDTRQWITSIAHALSLDD
                     SRVHEVLNSEQGAIQARWDELSAIARKNTLGFVVDQRVLPRLTDPDRSFGLSLARIVR
                     EMGFGIEVLCYAPEGDTSYARHQLSDMPVRLTPFQTPLQLQQRLREGSFRAVYSDRTL
                     DRRLTRAGQSQFSYADFEMGLAGALRSLHRLVRACALPFYHRYASLLAGEQG"
     gene            complement(3969..>4407)
                     /locus_tag="PK710_20415"
     CDS             complement(3969..>4407)
                     /locus_tag="PK710_20415"
                     /inference="COORDINATES: protein motif:HMM:NF012369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12152.1"
                     /translation="IVTSEETMSLYAANNIAKAVVRYSRNGIMLAGLIANQREIGDFS
                     DMLNAFAESLGTRLCALFHRDPLVRKAENRFQTVVEAAPDSKISLQFQELAAQMALRE
                     PQPYPIPTPLDQNQFVQFIRQYSRDEDLQDEPSDGNDNASASS"
BASE COUNT          931 a         1172 c         1366 g          938 t
ORIGIN      
        1 aaaccttttc gggaaagatt accacctttt tggcgaaata tgatgtggat agcgctcttc
       61 tttggtccca cgccttcccc agtgcttcct tcgccgtatt atacggtgtt accgccgggc
      121 ccaacgatcg ggttgccgtt acgggaaggg tcgagggaag cgttgattgg ggcggtgggc
      181 ccctgagcgt caaggggcag gccgactgtg ctgtggcggt ctacgatgca acgggaaaac
      241 atgaggcttc cgtgttaatt ggggagaagg gagatgccaa gggcgacgat attcgtttta
      301 cgtccgacgc gatcctgata gtcggagccc attcggggcc gctgtctttc cgtggctcgt
      361 ccctgctcga agcttcagac caaaacgcct tcgttcttgc cctatcccct gatttatcct
      421 cggtgccatg ggccttttcc cttggagcgg agggcgaaga ccaggcgctc ggcttggata
      481 ctcgcggaaa gtacgtcctc gtcgcgggag agtttaaaaa ctctttgaaa tacaaacagt
      541 taagtgctac atcgcaggac gtgggccagg cttttcttct tcagttcctg ccaccgccta
      601 tcacgaatta gggggcacca ggccggacca tgggcatgga cttctgacga ccggttggga
      661 cacgaatgtc gcacccacgc ttgtggtgat acaggaacca tcctctcgaa gggcaagctt
      721 gtggaccggg gggggcggac ctgcgatttc agccgttgga ggggatgggg cggagatcac
      781 gcaacaaatg atgccatgcc tgacgttcca cccatcgggc caccagcgac aaacagcctc
      841 gaaatcccat aaaaggctgg tctgtgaggg cgtgcgaatg aaatgagggg aatccgagtt
      901 caaaagcggc cacggggggg acgagatcct gcaatacata actggtggcg accaccagat
      961 tcacaccgcg tttttcgatg gcgacccgca acggctcttg aagctgtcct tctttcggat
     1021 ccacgatggt gatgggatcg tccagcggat cgggttggag ccagtcctgt ccatcattct
     1081 gtacggcgct gacaatggcc cgcagaggca ctggaatacc caattcgcgc gcaagaccga
     1141 gcaaaccccg gacaagatac ggttctccca tcagcgccag actcatatca cggctgacaa
     1201 gcgatacgac cgcttccaac ttggttgcgg cttgcgatag ttccttgtgg acgaaagcct
     1261 ctgccgcgcg ttgtgttccg agggctcgag ccaccgtttc taaccaggtg cgggtgcctt
     1321 cgaggccaat gggaagctcg gtttccacca acgcggcgcc ggtgcgattt gccaatattt
     1381 tcgcggcatt acgtgcgtag gggagcgaaa tgacaacatc cgcctggccg attttggcca
     1441 gatgcgtcgt aggctttccg ctcaaccagg tgcagacgag cgttccgccg atcccttcga
     1501 tcagccgctc cagttctcgc aaattaccga aatggtcttc ctcatttcgg tccatcaagt
     1561 agcccacgat ggcgacgcgc ccctggttgg ctccttgccg tggttcaaga ggcaagtatt
     1621 tcgcaagata ttcgagggta ttgtcgtagc ccgtcagcca atcatccacc aaagaacgcg
     1681 taggaagcaa aatcaaaggc ttttccgtgc cggtttgctc cgcgcaagcg tcgaggatga
     1741 gttcgtaggg aggttccgtg atcgacgcca tccgaatggc ccccaaaaaa atcacccggc
     1801 tatcggagcg tccgagcgca tacgatacca cgtctgaaat ggctttttcc cggttttgcc
     1861 agacctcttc cggcatcgcg tgggtggtga ggattcggtg ggcgccatcg gcggaaatca
     1921 gattcgattg ccaatcgtga ttgccctgga tctgggaagc catttgcacc gtgcaatcca
     1981 tcccatccat cacgagcaag gcgtcgggaa gcgcgttgat ggccagataa acacccaagg
     2041 agtagctgaa gccaagacga atatcgaatt cggagtggtt catccttgct cccccgccaa
     2101 taatgaggcg tagcggtgat aaaagggtaa tgcacaggcg cgcacgagcc gatgcaagct
     2161 ccgcaacgcc ccggccaggc ccatctcgaa atcagcgtag gaaaattgcg attggcctgc
     2221 tcgggtcaat cgacgatcca gggtgcggtc gctgtaaacc gcccgaaaag agccttcccg
     2281 aagccgctgc tggagctgta gcggggtctg gaacggcgtg agccgcaccg gcatgtccga
     2341 aagctgatga cgtgcatacg aggtatcccc ctcgggcgca taacaaagca cttcaatacc
     2401 aaagcccatt tctcgaacga ttcgtgccag tgacagtccg aaagagcgat ccgggtccgt
     2461 cagacggggc agcacccgct gatccaccac gaatccaagg gtgttttttc gagcgatggc
     2521 actaagctcg tcccaacggg cctgaatggc accttgttcg ctgttgagaa cctcgtggac
     2581 ccgtgaatcg tcgagggaaa gcgcgtgggc aatcgacgta atccactgcc gggtgtcgtc
     2641 aattccgtaa ggggcgggag gggtcacgat ctgatccgcc atggcgtcaa aaacttcatg
     2701 gtacagtcgg gtgtagttgg gattgggaaa acgcacttgc aagttgggtt tcggggtacg
     2761 acggcacacc gctacatcga tatcgggcag aagcagggca ttgacgacgg caccgcagcg
     2821 ctcgagaagc gtaaccaatt ccagcgtgtc ggggccgtcg ggaaagccta cgaggtcgat
     2881 gcgcttccca tccggttcct gctccaggca acggccttgg gggggtagca aaagatcgcg
     2941 catcacctgt ggttcgtctt gaccgaattc gagttcgtct gtgtacagac tggtgtcatg
     3001 ggtgagagga agagcctggg acaggcaatg aaccttcggg gtattgcgag ccagggaaat
     3061 ggcgggcccg acgtcttctc ccatgacttt gggcaggcag gtggcgttga tggcgacgat
     3121 acgttcacct tggccggtgg cggcgagggc gacggcttgc atgagtttgt cttcggcccc
     3181 gcgcacggta tcgacctcgt tcatattggt ggatattctg cggaacggtt ggtcgaggta
     3241 ttggggcccg agaccgagac gggcgcggag ccaatcggcc gcgggacgat gggggcgtcg
     3301 tggatggtag aagagaaaaa acgaacgcgt gcgtattttc ggtgtgacgc aggtgcattc
     3361 atggcagccg tggacgacgt gggtggtggg caatacgaag ttgacccgcg acaatgccgt
     3421 gaacacgaac accagatcgt cgaaaaatag accccattgg tcgacctcac caggttttcc
     3481 cgtccatgta tgcgcatagg gtttgggggc gaggttggct ccggcgcgca ggccgccgag
     3541 caatccttcg gaaagcactc cgctcatggc ttcggttttg gggtcgcggc gaagccagtc
     3601 cagcaggtct tcaatcggtg tggccttgag ccgattgtgg aggaaatcca cgagacgttc
     3661 cacctgcggg tggctgggcg catccgtaga ttcagccttt accgtcagtt tttcctgacc
     3721 gggggtgtgc gtcggttgaa gatgaatcat ggcccggccg aaaagccgtc ccgtaaggca
     3781 aacgcgaagc atcccttctt tccaggtgat gctggacagg acataggttc cgttggctcg
     3841 aagtgtcgga gagataccaa aaacgtggtg gaacgcggcg acggcatcgg ggccagcgac
     3901 cagatatctt ggaatcctcg atagatcgga agtcatggtc ttgtatcgcc acgccccttc
     3961 tgattcacct acgatgaggc agaggcgttg tcgttgccat cggatggctc gtcttgcaag
     4021 tcttcgtcgc gagaatactg ccggatgaac tgcacgaact ggttctgatc caggggagta
     4081 gggatgggat agggttgcgg ctcccgaagg gccatttgtg ctgcaagttc ttgaaattga
     4141 agggatattt ttgaatccgg ggcggcttcc acgacggttt gaaagcgatt ttcggccttt
     4201 cgtacgaggg ggtctcgatg aaatagggcg cagaggcgtg tgccgaggct ttcggcgaag
     4261 gcgttcagca tatcggaaaa gtcacctatt tctcgctggt tggcgatgag acccgccagc
     4321 atgatgccgt tgcggctgta gcgaacgacg gctttggcga tattgttggc ggcgtataac
     4381 gacatcgttt cttcggaggt cacgatg
//
LOCUS       DAOSOR010000498         4406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_19897_length_4406_cov_4.617100, whole genome shotgun sequence.
ACCESSION   DAOSOR010000498 DAOSOR010000000
VERSION     DAOSOR010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19897_length_4406_cov_4.617100"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PK710_20420"
     CDS             <1..193
                     /locus_tag="PK710_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12153.1"
                     /translation="PVLQALDAKGAQGVWEVLERIERELRAVMLLVGAANVEALRAAP
                     KFLSPELRPWLLGESNDRR"
     gene            278..1123
                     /locus_tag="PK710_20425"
     CDS             278..1123
                     /locus_tag="PK710_20425"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOT12154.1"
                     /translation="MSSAPPVPPPSERPASFNKERDEFIQTFFHKGARFTQELLQENE
                     QLSQRLSDLEAENAAMRLQIKSDQAIRELLTKIAQLEEEKAKLLSAVREVEAKSSAVE
                     TTYSEVEMELANLAGLYVASRQLHSTMNVSSTVKQIKEILEQLVGAKAYAVYFLSDDA
                     ASLVCIASQGLDEQASRLDQHLGPEGKVFRTGEAYISEDGDLASRPLSDPAACIPLRI
                     ADRVVGVITIVATLEQKPKFLPVDYEYFKLLSGHAASAIVAARLFANANHQVPGIESF
                     LEHGV"
     gene            1133..1510
                     /locus_tag="PK710_20430"
     CDS             1133..1510
                     /locus_tag="PK710_20430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT12155.1"
                     /translation="MAEYACLIIEDSPMMRQLLVFALARVRNLRVTEADDGVDGLRKL
                     ANARFDLIVTDINMPIMDGLKLIRRVRTDPVHKDTPIIVITTEGAQEDRHRALQLGAN
                     AYITKPIQAPQVIAKVKELLGVS"
     gene            complement(1530..2411)
                     /locus_tag="PK710_20435"
     CDS             complement(1530..2411)
                     /locus_tag="PK710_20435"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HOT12156.1"
                     /translation="MINNRSSHGESSDSVSRYISYVRDIDQFSREEELEIARKWVSEG
                     DRCAADALIRANLRYVVAIALTYRRYGLPLADLIAEGNFGLVHAVSKFNPERGTRFVT
                     YAAYWIRAYMLNYVIRSWSMVGVGSGPLRSKLFFRLRRERARIANLVGDNEEATRILA
                     ERFGESQDKIVSLANRLEARDVSLDARVFDDGSIAILDTLESDEPSQEETVEHQQSHD
                     RIKASLREALHDLDPRERFIVEVRMMADADDALSLAEIGRRLGVSRERARQLEARAKK
                     KLRDRLNHLRESEIVAA"
     gene            2649..3200
                     /locus_tag="PK710_20440"
     CDS             2649..3200
                     /locus_tag="PK710_20440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12157.1"
                     /translation="MKHRFRKQQTIVAALVLAVGGFAFSALAGRKADDRSVAMAVLSQ
                     RVSSSAIPASRQALTQAQHALFRADQARAAGDEVNAKLLEGLAREWAELATDSERAAQ
                     AELRADQSQKAAASASAEVRKTQALLETLTARRARAEGQLQQAQESSSASIPSAALSA
                     ARLRPPRPASAASATGSQPGGQP"
     gene            3197..4291
                     /locus_tag="PK710_20445"
     CDS             3197..4291
                     /locus_tag="PK710_20445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12158.1"
                     /translation="MKRLWLVGGLSLSLCGCGHPPRPTALSAADQAARSPTVQDAAPL
                     APQLLAHAEGLRTQAQASYERGEIASAGLLAERAMVAYERAAVMARLIRAEKLAAQAQ
                     NDLSDTTQKQQPLEAERQRLEADIAAIEQLILVVRDAPPITPSGTTDPSRELARLTAA
                     RSIIVDARLLCSAAQLLDPPMEGLSPATAEVTRLEQLLAQWPRPAPVDETMRARTTCL
                     SLLTLARTAHPSTLATDVVLAELSENPDLQPSRDDRGIAITIKDDPQTNPSTKANVQR
                     IAIISKKYKDFPILLVSHTRAKAPVAVQTTMRNRMQTIADTLAAEGIDRSRIVQIEAG
                     SNRPIAHDPLPPPVPSQNDRVEIVLVSPCL"
BASE COUNT          984 a         1323 c         1217 g          882 t
ORIGIN      
        1 tccggtgctc caggcgctgg atgcgaaagg agcccagggg gtgtgggagg tattggagcg
       61 aatcgagcgc gagttgcgcg cggtgatgct tttggtggga gctgcgaacg tggaggcatt
      121 gcgtgcggcg cccaaatttt tgtcccccga gctacgacca tggttgcttg gcgagtcgaa
      181 tgatagaaga taaggattcc gtgtcggtat ggttgatggt tcgaggctag ttcgactgtg
      241 atacgctcga gtaccctttg attggatcga aactctcatg agcagcgcgc ccccagttcc
      301 tcctcccagt gaacgtccag catcgttcaa caaagagcgc gacgagttca tccagacgtt
      361 cttccacaag ggagcgcgat tcacgcagga gttgcttcaa gaaaatgagc agttgagtca
      421 acggctttcg gatctggaag ctgagaatgc ggcgatgcgt ctgcaaatca aaagcgatca
      481 ggccatccgc gaattgctta cgaagatcgc tcagctcgag gaggagaaag ccaagctact
      541 gtccgcggtg cgtgaggtcg aggcaaagtc ctccgccgtg gaaaccacct attccgaagt
      601 ggaaatggaa ttggccaatc tcgccgggct gtacgtggca agccgtcagc ttcactcgac
      661 catgaacgtg agcagcacgg tcaaacagat caaagagata ctcgagcagt tggtgggagc
      721 caaagcgtac gcggtatatt tcttgtcgga tgatgctgcg agcttggttt gcattgcgag
      781 ccaggggctc gatgagcaag ccagccgatt ggatcaacac cttggcccgg aaggaaaagt
      841 attccgaacg ggtgaggctt atatctcgga agatggggac ctggcttcgc gccccttgtc
      901 cgacccggcc gcatgtattc cccttcgcat cgcggaccga gtcgttggcg tgatcaccat
      961 cgttgccacg ttggaacaga aacccaagtt tcttcccgtc gattacgaat acttcaaact
     1021 gctttcggga catgcggcga gcgcgatcgt ggcggcacga ttgtttgcca acgccaacca
     1081 tcaagtccca gggatcgagt ccttcctcga gcacggagtc tagtcaaaga ccatggctga
     1141 atacgcatgc ctgatcatcg aagactcacc catgatgcgc cagcttttgg tgttcgcgtt
     1201 ggcgagagta cgcaatcttc gtgtgaccga agccgacgac ggtgtggatg ggcttcgaaa
     1261 gcttgcgaat gcaagattcg atttgatcgt cacggacatc aacatgccca tcatggatgg
     1321 tctcaaactc attcgaagag tgcgcaccga cccggtacac aaggatactc cgatcattgt
     1381 gatcacgacg gagggagcgc aagaagatcg ccatcgggcg ctgcaacttg gcgcgaacgc
     1441 gtacatcacc aagccgattc aagcgcctca ggtgatcgca aaggtcaagg aattgttggg
     1501 agtatcataa aaacgcccgc gcggagtcgt caggcagcaa cgatttcgct ttcgcgcaag
     1561 tgattgagcc gatcgcgaag ctttttcttg gcacgcgctt cgagctggcg agcacgttct
     1621 cgggataccc ccaaccgccg accaatctcg gccagagaaa gagcatcatc cgcatccgcc
     1681 atcatacgga cttcgacaat gaaccgttcg cgcggatcga gatcgtgaag agcctcgcgc
     1741 agactcgctt tgatccgatc atgggattgt tgatgctcca ccgtctcctc ttgactgggc
     1801 tcatcggatt cgagcgtatc caaaatcgca atacttccat cgtcgaatac gcgggcatcc
     1861 agcgagacat cccgggcttc caaccggttg gcaaggctga cgatcttgtc ttgggactcg
     1921 ccaaaacgtt cggcgagaat acgggtggcc tcttcattat cccccacgag gttggcgatt
     1981 ctcgcgcgct cccgtcgcaa tctgaaaaac aacttggagc gcaacggacc ggagccaacg
     2041 cccaccatac tccaagaccg aatgacgtaa ttgagcatat aagcgcggat ccaataggcg
     2101 gcgtaggtga cgaaacgggt tcctcgttcg ggattgaatt tgctcacggc atgaacgagg
     2161 ccgaagttgc cttcggcgat gaggtccgcc agcggcagcc catatcgccg gtaggtcagc
     2221 gcaatcgcga ccacataacg caaattggct cgaatgagcg catcggcagc acaacgatct
     2281 ccttcactga cccattttct agcaatttcc agttcctctt cccgagaaaa ctggtcgatg
     2341 tctctgacat acgaaatgta ccgagaaact gagtcggagg actcgccatg ggaggaccga
     2401 ttgtttatca tgttacgtta cgtaatatta ttttcagagc cttgggtcaa gcgacagcac
     2461 aaccatcgcg gatccggaga cttttgcgtc agcggcagaa gatctcgtgg agtccaatgc
     2521 cattcgatgg tgcaacgccc cttgacgttg cgcggtttcg caccgaggga ctgacttatt
     2581 attttttgcc ttatcgaacc ggttcttctt gaccacgggc ccgcctcatg cgttgttggc
     2641 cccgtcccat gaaacaccga tttcgtaagc agcaaaccat cgtcgcggcc ttggtccttg
     2701 ccgtgggagg ctttgccttt tccgcgttgg cgggccgtaa ggccgacgac cgctccgtgg
     2761 cgatggccgt gctttctcaa cgcgtttcct cttcggccat tccggcgagt cgtcaggcgc
     2821 tgacccaagc ccagcatgct ctttttcgag cggatcaggc acgggccgcg ggcgatgagg
     2881 tcaatgcgaa actgctcgag ggattggcgc gagaatgggc tgaattggcg accgatagcg
     2941 aaagagccgc acaagcggaa ctccgtgcgg accaaagcca aaaagccgcg gcgagcgcat
     3001 cggccgaggt gcgaaaaacc caggctttgc tcgaaacgtt gacggcccgg cgcgcccgag
     3061 ccgaaggaca attgcagcaa gcccaagaat catcgagcgc atcgatcccc tcggcggctt
     3121 tatccgccgc aaggcttcgt ccaccgcggc cagcttccgc cgcttctgcc acgggcagcc
     3181 agccgggagg tcagccatga aacgcctgtg gctcgtaggc ggcttgagcc tttcgttatg
     3241 cgggtgtgga cacccaccgc gtccgacggc cctgagcgcc gccgaccaag ccgcgcgttc
     3301 acccacggtg caagacgccg ctccgttggc tcctcaactc ttggctcatg ccgaaggctt
     3361 gcgtacacaa gcgcaggcat cgtatgaacg aggcgaaatc gcttccgcgg gattgctggc
     3421 agagcgcgcg atggtggcct acgaacgcgc tgccgtcatg gcacgactca ttcgcgcgga
     3481 aaagctagcc gcccaggcgc aaaacgacct gtcggacacc acgcaaaaac agcaaccact
     3541 cgaggccgaa cgtcagcggc tcgaagccga tatcgccgcc attgaacaac tcatcctcgt
     3601 cgtacgcgat gcccccccca tcacccccag cggcaccacg gatccgtccc gggaattggc
     3661 gcgcctcaca gcggcccgct ccatcatcgt cgatgctcgc ctgctatgtt cggcagccca
     3721 actgctcgat ccaccgatgg agggtctgtc accggccacc gccgaggtca cgcgactcga
     3781 acaactgctg gcccaatggc ccagaccggc gccggtggat gaaacgatgc gagcgcgaac
     3841 cacgtgtttg tctttgctca cgttggctcg caccgcgcac ccttccaccc tcgccacgga
     3901 cgtcgttttg gcggagcttt cggaaaatcc cgatcttcaa ccctcccgcg acgatcgagg
     3961 gattgccatc accattaaag atgaccccca aaccaacccc agcaccaagg caaacgtgca
     4021 gcggatcgcc ataatctcca aaaaatacaa ggattttccc atactcctgg tgagccatac
     4081 ccgcgccaaa gcacccgtgg ccgtccaaac caccatgcga aatcgcatgc aaacgatcgc
     4141 cgatacgctc gccgccgaag ggatcgatcg ctcgcgaatc gttcaaatcg aagccggatc
     4201 gaaccgtccc atcgcccatg accccctgcc tccgcccgtc cccagccaaa acgaccgcgt
     4261 ggaaatcgtg ctggtttccc cttgtctata acgatttgta attttgatga aataataatt
     4321 attcacttcc actttacgag tcaccccaaa ggactcactc ttaacatgtg acgcttcctg
     4381 ttcatcccac cgagtcggcg cccccg
//
LOCUS       DAOSOR010000499         4393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_19963_length_4393_cov_4.310051, whole genome shotgun sequence.
ACCESSION   DAOSOR010000499 DAOSOR010000000
VERSION     DAOSOR010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19963_length_4393_cov_4.310051"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2198)
                     /locus_tag="PK710_20450"
     CDS             complement(<1..2198)
                     /locus_tag="PK710_20450"
                     /inference="COORDINATES: protein motif:HMM:NF012588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HOT12159.1"
                     /translation="MSDELHDFSATGSWIVPQPSRLLVDLEHAQGKEQALVRMVRRLG
                     LSVVVDDASRIRAGTWVDKATVRTEWGQITLQFMQVQTRKPDPLGTVLELRTDDPRSV
                     AALWSYLYHRRQLSSGEPWVGEKRDYEELRVPRRAKHAKETWEDRAAWLREVFGQDFP
                     ALIGARLNPQDVEGNIENYIGAIEIPVGLAGPMMFHGRYARGMILAPMATSEGALVAS
                     TNRGARAVTAAGGVTTHIIHQRMIRAPVFVTDRLESALRLQAWIMDHEPEIRSQVNQV
                     GHHTRLVQLDPVLIGRNLHLRFLFETGDAAGQNSSTLATWRACQWIRAQTAYLPDLPI
                     EHFFVEANTSGDKKATSLNYVMGRGFRVTAEALLPPIALSRLRVDADTLLRLRDSAIT
                     GSAYAGMMGMNANFANVVAAMFAATGQDIASVHESSVGQFHLERHKDGVRAYLMLPNL
                     VVGSVGGGTRLPGQRECLQIVGAYGRAKAARLAEIIAGYCLALDLSTLSAIAAGHFAT
                     AHESLGRSHRQSERLEPSLLSDLCSRAVRKWLRLEPMDELKIHAVVPVQGGTEDSFMG
                     SLDAVGKQRHAVVEASWTMDNTAGSSRFLVKSKPTDHEVVRMMRAVAMLCGEPLASLY
                     EVYQYRLGLRNCHVRELEMAQINDPRFTSITPEFLTVWRDDTHATYLIVMEYLENVTH
                     LNSVKQPQIWKQEHIVAVLHDIARFHSMYLGREADLERVEWLEIPSAPAK"
     gene            2637..3494
                     /locus_tag="PK710_20455"
     CDS             2637..3494
                     /locus_tag="PK710_20455"
                     /inference="COORDINATES: protein motif:HMM:NF014078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HOT12160.1"
                     /translation="MSSQAIRQPRKRLFFGLAALAWLLTAAAGLLLWRITAPGLGQLH
                     AALPIASALIIGAGVAALGLGTLGMVLATLGFRPPVQVARTFAWWAINLVFRPVMLLG
                     RLFDIERERIERSFIEISNHIVRGRHIEVFPNQLLILLPHCLQLDRCRHKITHRIENC
                     RRCGVCPIGDLARLAEYYEVNIAVVPGGTLARKVIKSLKPRAVLAVACERDLVSGIQD
                     VFPMPVIGVLNDRPQGPCCNTIVDLDALERGIRSLLRPQSLPPPASLSRPPRSSGQPR
                     VDPPCLSLH"
     gene            3540..>4393
                     /locus_tag="PK710_20460"
     CDS             3540..>4393
                     /locus_tag="PK710_20460"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT12161.1"
                     /translation="MNYHPTLRSLRLFYFFSFGALGSLFPFLPLLLHKRGLGPSQIGW
                     IMMVIPFANVVIPPIWGFAADAYRIRLPLLRLAATGSALTCLLLLTQHGFFATLAVVA
                     LFSLFRAPLTSLADAASVASLDGRSHEFSRIRVFGSVGFGLFVATLGWLDATDHPSLL
                     IASTAVVYLLATATTLSLSTPSTVSKTGMSSDVRRVLGRPSVLVFLAMSAVYYAGHAI
                     FDVYFGLRMASLGLGDSVVGAGWAIGIASEIMVLLLAPRFFRFVRMEIWLTVCAVAAA
                     LRWLGIAAL"
BASE COUNT          865 a         1336 c         1171 g         1021 t
ORIGIN      
        1 tgttttgcgg gagccgaagg gatttccagc cattccacgc gctcgaggtc cgcttctcga
       61 cccagataca tggaatggaa tcgggcgata tcatggagta cggcaacgat atgctcctgt
      121 ttccaaatct ggggttgttt gacggaattc aagtgggtaa cgttttcgag gtattccatc
      181 acgatcaggt aggtggcatg cgtatcgtct ctccaaaccg tcaaaaattc gggtgtgatg
      241 gaagtgaagc gaggatcatt gatttgcgcc atttcgagtt cgcggacatg gcaattgcga
      301 agcccaaggc ggtattgata aacctcgtaa agggaagcca aaggctctcc gcagagcatg
      361 gccaccgcac gcatcatacg gaccacctcg tggtccgtgg gcttggattt gaccaaaaat
      421 cgcgatgacc cagcggtgtt atccatggtc cacgaggctt cgaccacggc gtgacgctgt
      481 tttcccacgg catccaacga acccatgaaa ctatcttccg ttcctccttg caccggcaca
      541 acggcatgaa ttttcagttc gtccatcggt tcgagccgga gccatttgcg cactgcccgg
      601 ctgcataaat ccgatagcag ggagggttcc aatcgctcgg attgtcgatg gctgcgaccc
      661 aggctttcgt gggcggtggc gaaatgcccc gcggcaatcg ccgataacgt ggataggtcc
      721 agggcgagac aatagccagc gatgatttcg gcgagacgag cggctttggc gcggccgtac
      781 gctcccacaa tctgcaagca ttctcgctgc cccggaagcc tggttcctcc tcctaccgat
      841 ccgacgacaa ggttcgggag catcaaatag gccctcacac catccttatg ccgctccaaa
      901 tgaaactgtc cgacggagga ttcatgcacc gacgcgatat cttgtccggt tgccgcgaac
      961 atggccgcca ccacgttggc gaaattggca ttcatgccca tcattcccgc gtaggcagag
     1021 ccggtaatcg ccgaatcccg caatcgaagc aaggtatcgg catcgacccg cagacgactc
     1081 aacgcgatgg gaggcaacag cgcttcggcg gtcacccgaa acccgcgtcc catcacgtaa
     1141 ttcagcgacg ttgctttttt atccccactc gtattggctt ccacgaaaaa atgttcgata
     1201 ggaaggtccg gcaggtaggc ggtttgcgcg cggatccatt gacatgctcg ccacgtggct
     1261 aacgtcgatg agttctgccc cgccgcatcc cccgtttcga ataggaatcg caagtgaaga
     1321 ttgcgcccaa tgagcaccgg gtcaagttga acgagtctcg tgtggtggcc cacctgattc
     1381 acttggctac gaatttccgg ttcatgatcc atgatccagg cttgaagccg tagggcggat
     1441 tccagacggt ccgtcacaaa aaccggagca cgaatcattc gctgatgaat gatgtgggtg
     1501 gtaacccctc ccgcggcggt caccgcgcga gcgccccggt tcgtggacgc caccaacgct
     1561 ccttccgaag ttgccatggg agccagaatc attcccctgg cataccggcc atgaaacatc
     1621 atcggtccgg caaggcccac gggaatctcg atggcaccaa tatagttttc gatattgcct
     1681 tcgacatcct gcggattcaa tcgcgctcct ataagcgcgg gaaaatcctg accgaatact
     1741 tctcgaagcc aagcggctcg gtcctcccag gtctctttcg catgctttgc ccggcgtgga
     1801 acacgcaact cttcgtagtc gcgcttctcc ccaacccacg gctcccccga cgacaactgc
     1861 cgccgatgat ataagtacga ccatagcgcc gccacggacc tgggatcgtc ggtccgcagt
     1921 tcgagaaccg tgcccaaagg atcgggcttg cgtgtctgaa cctgcatgaa ctgcaaggtt
     1981 atctgccccc attccgtgcg aacggtggcc ttgtccaccc aggtgccggc ccgtatccgc
     2041 gatgcatcat cgaccaccac ggacaaacca agccgtcgca ccatccgtac aagagcttgt
     2101 tctttgccct gcgcgtgctc gaggtccacg agtagcctgg acggctgagg cacaatccag
     2161 gaccctgtcg cagaaaaatc atgaagttcg tctgacatgc cggctctctc agtcgatgca
     2221 atcaccacag cctatcccga ctcctaatcc aactgccaac cgttcgacag tccccaagca
     2281 tccgcggcgg ctcggcgtcg cctatttctc cgattccgtg cagtattttg atgcccccag
     2341 cgatgcaccc ccttcgtgaa gggccgaccg cggcgtatca tccttcaacc caataggcaa
     2401 aggtggcgcc ctccgaaacg cggtcgccgt cgaatatcgc tttcccatcg ataaaatcat
     2461 ttgtattttc aaatagatag gcaataccac ggctcaaaag ctccccaagc gttcccatct
     2521 cccatcgcaa gcgcgtggac gaacagtccg aatgtcatgg tgcaaacgaa aaggcatttc
     2581 gattcacgac cacggctgtt gcggtcgcgg caaaaagacg gctaatagaa atgaccatgt
     2641 cttcgcaagc cattcgacag cctcgaaagc gcctgttttt cggattggct gcactagcgt
     2701 ggctgctgac ggcggcggcg ggcttgctat tgtggcgcat caccgctcct ggattggggc
     2761 aacttcacgc ggcgttgccc atcgcgtcgg ccctcatcat cggagcagga gtcgcggctt
     2821 tgggtcttgg caccctgggg atggtattgg ccacgttagg ctttcgcccg ccggtacaag
     2881 tggctcgcac cttcgcttgg tgggcgatca accttgtatt tcgacctgtg atgctccttg
     2941 gacgcctttt cgatatcgaa cgagagcgca tcgaacgttc tttcatcgag atttcgaatc
     3001 atatcgttcg aggccgacat atcgaagtgt ttccgaatca attgctcatt cttcttcccc
     3061 attgcttaca actcgatcga tgccgacaca aaatcacaca ccgtatcgaa aactgccgac
     3121 gatgtggggt atgccccatc ggtgatctcg cgcgtttggc agagtattac gaagtgaata
     3181 ttgcagtcgt tccgggaggc acgcttgccc gcaaagtcat caaatccttg aaaccccgag
     3241 cggtgcttgc cgtggcttgc gaaagggacc tggtcagcgg catccaggac gtttttccca
     3301 tgccggtcat cggcgtgctc aacgatcggc cgcaggggcc ttgttgcaat acgatcgtcg
     3361 acctggacgc tctcgagcga gggattcgaa gtctgcttcg tccgcaaagc cttccgccgc
     3421 cggcatcgct cagtcgcccc ccgcgatcga gcggccaacc acgagtcgac ccaccatgcc
     3481 tttctttgca ttgaccttcc cttgcatgga tttccattcc atgcttggag ccctgcgtct
     3541 tgaactacca tccaacgcta cgttccttac ggctctttta cttttttagt ttcggcgccc
     3601 tgggatcgct tttccctttc ctacccttgt tgctgcacaa acggggcctc ggtccgagcc
     3661 aaatcggttg gatcatgatg gtgatcccct ttgccaacgt cgtgattccc ccaatatggg
     3721 gatttgctgc ggacgcctat cgcattcgtt tacctttgct ccgcttggcc gcgaccggaa
     3781 gcgcgctgac atgcctgttg cttctgaccc agcacggatt tttcgcgact cttgcggtgg
     3841 tcgccttgtt ttctctgttt cgcgctccgc tcacttccct tgccgatgcg gccagtgtcg
     3901 cctctctcga cggtcgcagc catgaattca gccgtattcg cgtttttggc tccgtcggtt
     3961 tcgggctatt cgttgcgact ctgggatggc tcgacgccac cgatcatcct tccttgctca
     4021 tcgcatcgac cgcggtcgta taccttctgg cgacagccac caccctatcg ttgtccacgc
     4081 cctccaccgt ctcgaaaacc ggcatgtcca gcgacgttcg aagggtgctt ggtcgtccat
     4141 cggtccttgt gtttctcgcc atgagcgccg tttactacgc ggggcacgct attttcgacg
     4201 tgtatttcgg cttgcgcatg gcgtcgttgg ggttgggcga cagcgtggtg ggagccggat
     4261 gggccatcgg tatcgcttcg gaaatcatgg tgttgttgtt ggctcctcga ttctttcgat
     4321 tcgtcagaat ggagatttgg ctgacggttt gcgccgtggc cgcggcgctg cggtggttgg
     4381 gaatcgccgc cct
//
LOCUS       DAOSOR010000500         4375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_20070_length_4375_cov_3.772454, whole genome shotgun sequence.
ACCESSION   DAOSOR010000500 DAOSOR010000000
VERSION     DAOSOR010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20070_length_4375_cov_3.772454"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..1257)
                     /locus_tag="PK710_20465"
     CDS             complement(46..1257)
                     /locus_tag="PK710_20465"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOT12162.1"
                     /translation="MLWTVKKGLYRTQQRVMKVAAKVLPFPVPALLTGPGSVRQLADN
                     VSVRGLRHVLVVTDKGLMDIGLPASLFESLREKGITFAVFDEVQVNPTIENVEAGVRV
                     YRQHGCDGIIAIGGGSPMDCAKIIGARVGNPFLSVRRMKGLFKVILPIPPLFCVPTTA
                     GTGSETTVVGVITDASTHEKFAINDLKLVPKVAVLDPELMVGLPPAITAATGMDALTH
                     AVEAYIGLNGNSFTDRHAEKATRLVFENLEKVYCDGSDLEARYQMALASTFAGAAFTR
                     AYVGYVHAIAHNMGGLYGVAHGLANAIILPHVLTFCREQAAHKLAMLAMVAGLGARGE
                     PDIELADRFIERIKQMNERMGIPTTIAELQAHDIPLIVRRTLAEAHPDYPVPRIMTYS
                     ECEKLVRQLLP"
     gene            1390..>4375
                     /locus_tag="PK710_20470"
     CDS             1390..>4375
                     /locus_tag="PK710_20470"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOT12163.1"
                     /translation="MTALGLHESHIAPKSSAELSLPILRVQRSCVHDGPGLRTVIFFQ
                     GCAMRCLWCHNPEAQPMRPCKGTTPTAISTILEEVCRDKAYFDATHGGVTLSGGEPML
                     QPPESLVALLQALHTKAIHVTVETAADAPWPSFQACLPWVDLFLVDLKTAGSPALHRR
                     LTHRPPDRVQDNIRRLVAANANVRFRMCIVPGHNDSDESLLGVARWLFALERPEIELL
                     RYHGLHERKARQLHLAQQPLAIDDHQAAKAFENAKLKFLQFGINIINSSSEVDSPKTP
                     FSPRIRAITKDLRDAKHAVCLESARLKTEFYKQNAFRPPAILHRARALQYVLAHKTIR
                     IYPHELLVGNFTAKRVAGNVWTEYFATSMAIHFWQIDRQKPVPFSCSTTEKIAFYKNL
                     LPFWARHSLIAKAFPKAKDFLPLLARTIEQRVGFNNNLAALAHFIVPVERLLRLGTSG
                     LAREIRQTQKDASPAPSDFYEATLIALQSVEAWAQRYAQHLEALARKETDALRRSELE
                     EMAAVCSHVPRHPARTFHEALQSILFVHIALCTESFENAISLGRLDILLQPYFERDRA
                     AGRLDRDKARELLACFVLKFDEIVFLNDGDFAFQLGKLFESLSPVETITMGGTDEYGQ
                     DVTRDVTYLLLDICELRPIGVNMAARIHRDSPKDYLHRIAQVYLGGSPMPALFNDDVY
                     VPALLRQYPVTPAQARDYGIVGCVEPVANHDHFANTDCANINVVLPFLQALRGDSRRL
                     WRRGALGRADQIGLDWLHRNARHWPSHRLVSPALYRLRGITPPHDLDSLLERFQLRLH
                     ELVQDILSDHQRIESALARDFQTPLASSLFAGCVQSGKDLYEGGASLNSSGIQAVGIT
                     DVADSLLAIEKIVVKDKLFSLDEIVHAIDTDFETEQGKVIRTHLLAAPKFGNDDAQAH
                     QWVNRVLQIWVDALKTAKHTTRNATYVAGYYGLNVNRVYGQKTPSLPSGRLRGEPLAN
                     SLCPHHGMQRTDLTSALNAVAKV"
BASE COUNT         1011 a         1409 c         1025 g          930 t
ORIGIN      
        1 catcgaaggg cattgaatcg cgttgatcct gaaaaatgag cgtagttacg gcagaagctg
       61 acgaaccaac ttctcgcact ccgaatatgt catgatgcgc ggaaccggat aatccggatg
      121 cgcctccgcc aacgtgcgtc gaacgatgag cgggatatcg tgcgcttgca actcggcgat
      181 ggtggtggga atgcccattc gttcattcat ttgcttgatg cgctcgatga accgatcggc
      241 caactcgatg tcgggttccc cgcgagcccc cagccccgct accatcgcca acatggcaag
      301 cttgtgcgcc gcttgttcac gacaaaacgt cagcacgtgc ggcaaaatga tcgcattcgc
      361 aagaccgtga gccaccccat acaagccccc catattatgt gcgatcgcgt gaacgtaacc
      421 cacgtaggca cgcgtgaaag ccgcgccggc aaacgtggac gccagcgcca tttgatagcg
      481 ggcctcgaga tcgcttccgt cgcagtacac cttttccaag ttctcgaata ccaatcgcgt
      541 ggctttctcc gcatgacgat cggtaaacga attgccgttc agcccgatat acgcctctac
      601 cgcatgggtc agggcatcca tccccgtggc cgccgtaatc gcaggaggca accccaccat
      661 caattcggga tcgagcaccg caactttcgg aaccagtttc aaatcgttga tcgcaaattt
      721 ttcgtgcgtc gaagcatccg taatcacccc aaccacggtg gtttccgagc ccgtcccggc
      781 agtcgtcggc acgcaaaata gcggggggat gggcaatatc accttgaaca agcctttcat
      841 gcgacgaacg gaaagaaacg gatttccaac cctggccccg atgattttcg cgcaatccat
      901 cggagagccc ccaccgatcg caatgatgcc atcacaacca tgctgccgat aaacacgcac
      961 ccccgcctcc acgttttcga tggtgggatt gacctgcacc tcatcgaata ccgcaaacgt
     1021 gatgcccttt tcccgaaggg attcgaaaag cgacgccggc aagccgatat ccatgagccc
     1081 cttatccgtc acgacaagta cgtggcgtaa cccgcgaaca ctcacgttat cggcaagttg
     1141 tctgacactt ccaggcccag tcaatagggc agggacggga aaaggcagca ccttggccgc
     1201 cactttcatg acgcgttgtt gggttcgata aaggcccttt ttcacggtcc acaacatggg
     1261 caacacctcc cggctgcccg catggtacgg cgtcggtcat ccacaagcca gcggatgatt
     1321 caaagagatg gacttgattt atatattctg atgatacaga atttacaatc agatcatcag
     1381 gaggagccca tgaccgctct cggcttgcac gaatcgcaca tcgcccccaa atccagcgca
     1441 gaactatccc ttcccatcct tcgtgtgcag cggtcctgtg ttcacgacgg tccgggtctt
     1501 cgcacggtca tcttctttca aggctgcgcc atgcgatgcc tctggtgtca taaccccgaa
     1561 gcacaaccca tgcgcccctg caaggggacc accccaacgg ccatttccac gatcttagag
     1621 gaagtatgcc gcgataaagc ctatttcgat gctacccacg gaggcgtcac cctcagcggc
     1681 ggcgaaccca tgctccaacc ccccgaaagc ctcgtcgccc tgcttcaagc ccttcacacg
     1741 aaagccatcc acgttaccgt ggaaaccgcc gcggacgccc catggcctag tttccaggct
     1801 tgcttgccat gggtcgacct attcctcgtc gacctgaaaa ccgccggatc ccccgcgttg
     1861 catcgacgtc tcacccaccg tccgccagac cgggtccaag acaatatccg tcggctcgtg
     1921 gcggcaaacg ctaacgttcg ttttcgaatg tgtatcgttc ccggccacaa cgattccgac
     1981 gaaagcttgc ttggagtcgc tcgatggctc tttgcgctcg aacgcccgga aatcgaattg
     2041 cttcgctacc atggcttgca tgagcgcaaa gcgcgacagc ttcacctcgc gcaacaaccc
     2101 ctcgccatcg atgaccacca agccgcaaaa gcattcgaaa acgcaaaact taaattctta
     2161 caattcggaa ttaatattat aaattcatcg tcggaggttg acagccccaa aacccccttt
     2221 tctcctcgaa tccgcgctat caccaaggac cttcgcgacg ccaaacacgc cgtctgtctc
     2281 gaatctgcgc gtctaaaaac cgaattttac aaacaaaatg ccttccgccc tcccgcaatt
     2341 cttcatcggg cccgtgcctt acaatacgta ttagcccata aaacgattcg tatttatcca
     2401 cacgaattac tcgtcggcaa tttcaccgca aaaagagtag ccggcaatgt ctggaccgaa
     2461 tacttcgcca catcgatggc gatccatttc tggcaaatcg atcgccaaaa acccgtcccc
     2521 ttttcctgct ccaccactga aaaaatagcg ttctacaaaa acctgttgcc cttttgggct
     2581 cgccatagcc tgatcgccaa agcctttccc aaagccaaag acttcctccc gttgcttgcg
     2641 cgtaccatcg agcagcgtgt tggattcaac aataacctcg cagccctcgc tcacttcatc
     2701 gttcccgtcg aacgactatt gcgcctggga acgagcggtc ttgccagaga aatcagacaa
     2761 acccaaaagg atgcttcgcc cgcaccctcc gacttttatg aagcaaccct catcgctttg
     2821 caatccgtgg aagcatgggc ccagcgatat gcacaacacc tcgaggcctt ggcgcgcaag
     2881 gaaacagacg cgctccgacg ttccgagttg gaagaaatgg ccgccgtttg ctcccacgtg
     2941 cctcgccatc cagcgcgcac gttccacgaa gccctccaaa gcattctttt cgttcacatc
     3001 gccttatgta cggaatcgtt cgaaaacgcg atttccctcg gacgtctcga tatcctgctc
     3061 caaccgtatt tcgagcgcga tcgggcggcc ggacgtctcg atcgcgacaa agcccgtgaa
     3121 cttctcgcct gcttcgttct caaattcgat gaaatcgtct ttctcaatga tggagatttt
     3181 gccttccaac tcggcaagct tttcgaaagc ctgtcgcccg tcgaaaccat cacaatgggg
     3241 ggaaccgacg aatacggcca ggacgtgacc cgagacgtga cctatttgct attggatatc
     3301 tgcgaattgc gccccattgg ggtcaatatg gcggcacgga tccatcgaga tagccccaaa
     3361 gactatctgc accgcatcgc ccaggtctat ctcggaggct ctcccatgcc cgcccttttc
     3421 aacgacgatg tgtatgtccc agcccttctt cgccagtacc ctgtgactcc agcacaagcg
     3481 cgtgactatg gcatcgtggg ttgtgtcgag ccggtggcaa accacgatca ttttgccaac
     3541 acggactgcg ccaacatcaa cgtggtcctg ccatttcttc aagcattacg cggggattca
     3601 cgccgtcttt ggcgaagggg tgccctgggc cgtgccgatc aaataggcct cgattggttg
     3661 caccgaaacg caaggcattg gccatcgcac cgcctcgttt ctcccgcctt atatcgccta
     3721 cgcggcatta cccctcccca cgatctcgat tccttactcg agcgcttcca actgcggctc
     3781 catgaattgg tccaggatat tctttcggat caccaacgca tcgaatcagc cttggcccgg
     3841 gatttccaaa cccctttggc ttcatccctc tttgcgggct gtgtgcaatc cggcaaagac
     3901 ctgtacgaag gtggtgcaag cctcaattcc agcggcatcc aagccgtcgg tatcaccgat
     3961 gtggccgatt cgctcctggc catcgaaaag atcgtcgtga aagataagct cttttccctc
     4021 gatgagatcg tccatgccat cgacacggat ttcgaaaccg aacaagggaa agtcattcga
     4081 acccacctgc tcgccgcccc caagttcgga aacgatgatg cccaggctca ccaatgggtc
     4141 aatcgggttc tgcaaatatg ggtggatgcc ctgaaaaccg ccaagcatac gacccgcaac
     4201 gcaacctatg tcgccggtta ttacggcttg aacgtcaatc gtgtctacgg ccaaaaaaca
     4261 ccgtcgttgc cctcgggacg cttgcgcggt gagccccttg ccaacagcct ttgcccccac
     4321 cacgggatgc aacgaaccga cttgacctcc gcgctcaacg cggtcgccaa agtcg
//
LOCUS       DAOSOR010000501         4371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_20094_length_4371_cov_3.652456, whole genome shotgun sequence.
ACCESSION   DAOSOR010000501 DAOSOR010000000
VERSION     DAOSOR010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20094_length_4371_cov_3.652456"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PK710_20475"
     CDS             complement(<1..392)
                     /locus_tag="PK710_20475"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOT12164.1"
                     /translation="MDQKIFDELFVLEIANNHWGKVDRGLKIISTYNQIVRFNGVRAA
                     LKLQIRDVPSFIHKDFLGRTDIRYIKKTLDTALPFEGYAALVEAIRKGPCIPMATPFD
                     EASVDTCMKLGIPLLKIASYDINDWFLI"
     gene            complement(431..2239)
                     /locus_tag="PK710_20480"
     CDS             complement(431..2239)
                     /locus_tag="PK710_20480"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOT12165.1"
                     /translation="MIRVADFIAQTLVSRGIDQAFLVSGGGAMHLNDAIGACSGLSYL
                     ACHHEQACAIAAEGYARIAGKPALVNVTTGPGGINALNGVFGAYTDSIPMLILSGQVK
                     RETCMAFHTVPGLRQLGDQEADIAAMVRGITKYVAVVRDPNDILYHLERALALATEGR
                     PGPCWLDIPSDIQAASIDPSCLRRYDPAEDAPVWSRQGLESVCSEILHRLRTASRPVI
                     LAGTGIRLANAVQAFQRLVDKLRIPVTTAWTHDIVPTASPYFCGRQGTIGDRAGNFTV
                     QNSDLLLVLGSRLCIRQVGYHGKAFAREAFKIQVDIDPAELQKPTVSIDLPVCCHLLD
                     FISELEKQLDARGDDSPKHADWLAWCKERVSRYPVVQPRHREFHDGINPYHFFDRLSD
                     RLQDDDVLVCANATATIVPFQVVAMRGCQRMFSNSGSASMGWELPAAVGAAIARRGKR
                     VLCLAGDGSIQMNLQELQTIVHHKLPVKVFVLDNAGYLSIRSTQNNFFGRLVGAGPSS
                     GVSFPNMLDVARAFGIPAIEIEGRDFEASLSSVLASQGPCVGVVRLDPQQGFEPRSSS
                     KSLPDGRIVSAPLEDMYPFLERDELRSNLFIEEWKN"
     gene            2437..3549
                     /locus_tag="PK710_20485"
     CDS             2437..3549
                     /locus_tag="PK710_20485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT12166.1"
                     /translation="MPRPKTISVVTPCFNEQDNVYPLFERTRATLEALGPTYQWEHIF
                     IDNASTDGTIEELQKLAAKDRRVKIIINTRNFGVIRSPLHGLFQASGDAIVSLCADLQ
                     DPPELITDFVREWEKGFLIVAAVKKGSRESPFMYAVRKLYYRTLNHISETELINDFTG
                     FGLYDQKIIDLVRRTGDHYAFFRGLVADMGFPIARVEYIRPVRQHGLSKNRLYDLYDQ
                     AMQGIVKHSKIPLRLTTMTGFIVAFLSALSALGYLIYKIIYWDSFSVGIAPAVIGLFF
                     LSSIQLVFLGMLGEYIGAIHGRLFQNWLVIEKARINFENHTGQEPRLATSLPLESKEP
                     PGRLEEHADLHSHPLVAEAPQKADEEVPRNDMSEEG"
BASE COUNT         1008 a         1243 c         1175 g          945 t
ORIGIN      
        1 tcaatcaaga accaatcgtt gatgtcgtag ctggcgatct tgagcagcgg gataccgagt
       61 ttcatgcacg tatcgacgga ggcctcgtcg aacggcgttg ccatcgggat acaaggtcct
      121 ttccgaatgg cttcgaccaa cgccgcatag ccctcgaaag gcagcgccgt atccaacgtc
      181 tttttgatgt accgaatgtc cgtgcgcccc agaaaatctt tgtgaatgaa ggatgggaca
      241 tcgcgaatct gcaatttgag cgccgcgcgg acgccattga agcgtacgat ttgattgtag
      301 gtcgagataa ttttgagtcc tcgatccacc tttccccaat ggttgttcgc gatctcgagg
      361 acgaaaagtt cgtcgaatat tttttgatcc atggtctcga ccttcgcgcg ccatcttgca
      421 ttggcttcgc tcaattcttc cattcttcga tgaacaggtt gcttcgcagt tcgtctcgtt
      481 cgagaaacgg atacatatct tcgagggggg ccgagacgat ccggccatcc ggcaaggatt
      541 tcgaactgct tcgaggctcg aagccctgct gaggatcgag tcggaccacc cccacacacg
      601 gtccttgact cgcaagaacc gaagatagac ttgcctcgaa gtcccttccc tcgatctcga
      661 tggcgggaat accaaaagcc cgggccacat cgagcatgtt ggggaacgac acaccactgc
      721 tgggaccagc gcccacgaga cgaccaaaga aattgttttg cgtgctgcga atcgaaagat
      781 accccgcgtt atcgaggacg aatactttaa caggaagctt atgatgcacg atggtttgca
      841 actcctgcaa attcatctga atcgaaccgt cgcccgccag gcaaagcacc cgcttgcctc
      901 gtcgcgcgat cgccgcaccc accgcggccg gcaattccca ccccatcgag gcactccctg
      961 agttcgaaaa catccgctgg cagcctcgca tcgccacgac ttgaaagggt acgatggtcg
     1021 ccgtcgcatt ggcgcaaacc aacacatcat cgtcttgcaa acggtccgaa agacgatcga
     1081 aaaagtggta gggattgatg ccatcgtgga attcccggtg tcgcggctgc acgaccggat
     1141 agcgggatac gcgctctttg caccatgcga gccagtcggc gtgcttggga gaatcatcgc
     1201 cccgcgcatc gagctgtttt tctaattcgc taatgaaatc aagtagatgg caacataccg
     1261 gcagatcgat ggacaccgtt ggtttttgca attccgccgg atcgatatcc acctgaatct
     1321 tgaatgcttc gcgggcgaag gctttcccat gatacccgac ttgacggatg cagagcctgg
     1381 agcccaacac gagcagcagg tcgctgtttt gcacggtgaa gttaccggcc cgatccccga
     1441 tggttccttg cctgccgcaa aagtacgggg aggcggtggg cacaatgtca tgggtccagg
     1501 ccgtagtcac gggaatgcgt agtttatcga ccagccgttg aaacgcttgc acggcgttgg
     1561 cgagacggat gccggtgccc gcgaggatga cgggacgaga ggcggttcga aggcgatgaa
     1621 ggatctcgct gcaaacgctt tcgagccctt gacgggacca tacgggggca tcttccgcgg
     1681 ggtcgtagcg ccgcaagcac gaagggtcga tcgatgcggc ttggatgtcg gagggaatat
     1741 cgagccagca agggccgggg cgtccttccg tggcgagagc cagcgcacgc tcgaggtgat
     1801 aaaggatgtc gttggggtca cgaacgacgg cgacgtattt ggtgatgcca cggaccatgg
     1861 cagcgatatc tgcctcctgg tcgccgagtt gccgcaggcc gggcacggta tggaaggcca
     1921 tgcatgtttc tcttttgacc tgtccggaaa ggatcagcat cgggatagag tccgtgtagg
     1981 ccccgaaaac gccattgaga gcgttgatgc cgccggggcc ggtggtcacg ttgacgagtg
     2041 ccggtttgcc cgcgatgcgg gcgtagcctt ccgcggcgat ggcacaagct tgttcgtgat
     2101 ggcaggccag ataggacaga ccggaacaag caccgatggc gtcgttcagg tgcatggcgc
     2161 cgccgccgga aacgagaaaa gcttggtcga tgccgcggga aaccaaggtt tgggcgatga
     2221 aatcagcaac ccggatcatg gaagcataag catacttgat tcggggtcct gaggacgact
     2281 ttgcgccagc atcgcctggg gtggtggcgg aatgatcgcc tatgctcggt attcacccta
     2341 tcgaaatgcc gatcgcgata gaatgggtca tcgagcatgg atgcatcgac ttcattgcct
     2401 tgttgtgatc ccgattctgc aagcggggac tcccccttgc cgcgacccaa aaccatcagt
     2461 gtcgtaacgc cttgtttcaa cgagcaagac aacgtctacc ctcttttcga gcgcacccgc
     2521 gccaccctcg aagccctcgg tcccacctat caatgggagc atattttcat cgataacgca
     2581 tcgaccgatg gcacgatcga agaactccaa aagctcgcgg ccaaggatcg acgtgtcaaa
     2641 atcatcatta atacacgcaa cttcggggtc atccgctctc cgctccacgg ccttttccag
     2701 gcctccggcg acgccatcgt atcgctatgc gccgacttgc aggatccccc cgaactcatc
     2761 accgacttcg ttcgtgaatg ggaaaaaggc tttctcatcg tcgccgcggt caaaaaaggc
     2821 tccagagaat cgcccttcat gtatgcggtt cgaaagcttt actaccgcac gctaaaccat
     2881 atttccgaaa cggaactcat caatgatttc acgggtttcg gactatacga ccaaaaaatc
     2941 atcgacctcg tccggcgcac cggtgaccac tatgcgtttt tccgcggtct cgtcgcggac
     3001 atggggtttc ccatcgcccg ggtcgaatat atccggccgg tgcggcaaca cgggttgtcc
     3061 aaaaatcggc tctacgatct gtacgaccag gccatgcaag gcatcgtcaa acactcgaag
     3121 atccctttgc gccttaccac catgaccggg ttcatcgtcg cctttctcag cgccctatcc
     3181 gccttgggct acttaattta caaaattatc tattgggatt cgttcagcgt cggtatcgcc
     3241 cccgcggtca ttggcttgtt tttcctttcc tccatacaac tggtatttct ggggatgctg
     3301 ggagagtata ttggggccat ccatggtcgg cttttccaaa actggctcgt catcgaaaaa
     3361 gcgcgcatca acttcgaaaa ccataccggt caagaaccca ggctcgcgac ctctttgccg
     3421 ttggagtcaa aggaaccacc aggccgcctc gaggagcacg ccgacttgca ttcccatcct
     3481 ctggtcgccg aagccccgca aaaggccgat gaagaagtcc cccgtaatga catgagcgaa
     3541 gaaggataag taggcagtat gtcctgtcgt gaagccttat gcccctaagc catcccctca
     3601 tatttgctgg gcttttcctt cccggtcacg gtccacgatc acggtcacgg cgcacggcac
     3661 acaaaaatgg tcacgttcat cccatatcgt tcaccgcaaa acgccagtac aatgtttagt
     3721 agcccaggcc aactgttggg ggtgcttcaa gggggtcaag gaccgagcca aactcaacac
     3781 ccaagaatcc taagctcgtg ttcctttcca attcaggaaa aaatgagggg atctgtcagg
     3841 tgatgaccca ggtggatggg ggctcgataa ggtgcggcga tgggcaacga ggctgtctca
     3901 cgatttggcg caatcgaaat acactgaata aaaacggtag gatagagtga acgaggcgag
     3961 atatatcggg tgtgggttca agtcaaaccc catcatgacc agcttattgg acgtggcaag
     4021 cgtcccgcgc gtcggcggaa cacgttgatg gctaacccat tcgcagccac gttgaactca
     4081 tatccaccaa gctggacagc cttattaccc agcggtgtcc cgataataga gtgtttgcaa
     4141 actggcaccc caccctggct ctccccccct ttttcccccc accctggctc taccccccga
     4201 atcggggtgg aggggaatta aaacagtggt taattattta gctaaaatat tttgaaacta
     4261 gaaactagga acaagaaatt cgaattagat attattgaag tgcattttat caatcctgaa
     4321 ccaaccttga tcccactccg actccgcctc ccttccaccc accatgtggg g
//
LOCUS       DAOSOR010000502         4364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_20141_length_4364_cov_3.667904, whole genome shotgun sequence.
ACCESSION   DAOSOR010000502 DAOSOR010000000
VERSION     DAOSOR010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20141_length_4364_cov_3.667904"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3218
                     /locus_tag="PK710_20490"
     CDS             <1..3218
                     /locus_tag="PK710_20490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12167.1"
                     /translation="TTPTIEFIVPCGEAITVSLVVQDSHGLSSALVHSVEVKEQAGPW
                     VSINDCVAGDECGTLDRPWCSMTAAYQRWPLIRAGGATGLRVVRGRYEQGTKNKNGVP
                     IVSPGETLVWNDTAATTDPSVEAYASLGGSPTAAASCAKWDSDPEGVAVVTHEQSGFV
                     LNDDVDVELHRLCLVAKPTSLTALPMVSALTTAGNGSRFVDGIIWMDQVSAPLPARNF
                     GVWFSSFVPNNKVARFENSIVVAGSSTDESGGMLIGLTPPMPILFQGVVRIQDATVYF
                     GNAASSQATYGVLSRDDVTEVYSSTLAGGPGTDESYGVFAQDQDTLRIVGSEVFGQGL
                     ESTTGVFVDAVDKVRIVGSSLYGYPSASAPTTVTWATGLHAYSGRLIDVMANDASYPD
                     LDIVGAGAGVTASTAARGIYVESLQAGAQAGELTVVGPDTGSTARIRGGDSLLRSSGI
                     EIETGIKLTVRHLASITAGRVKSSTSPLPVTQFVAAAIRLRGAGGEANIENINEIRGC
                     DPACIDQGGAISQPEESPLLGIGLFADADTQISPPTKGAWNILVKGPSTIQGGSIEKT
                     NSSNKSVAELIGIRALGDYKTGHRFKVEQNVVVRGSVATSPNGLPSSSAGVRLARSRF
                     IAVAPVEIMGGPAHHTARGVWMNNAHTSGTVLPGAGATLDVSATSTDSFRIVGNPESA
                     DNLRPMAVYGVQDASNRLAIIANTVSLKAYLPTATVPWSAIEGGWAAINSQGAAVGAS
                     FSATANVVLEHVRMHGGVVPGGLVQSGLVMKDLSAAPGTRPVVNACLIEACGQVQGSS
                     HHPDCHQSLQAFSVGAIIDAKDELGWPLLFSNNLVFGGFNTRAGGYGSIGMVATVDND
                     PMGATNSPARHFINNFFSGQGTTPTASCSAGVNPAVSEGLRLKIGPSSSHNIQTTVAA
                     WRRNIFHDGGVACKRFAVSEQGIDADLKGIPFESNDYVKEIPGRDVFPVGSYVALYRE
                     AKYLGPIDHCIYGLPSLFPSCIDVDITNNTDYSNSLNVNPLFATSDVNALIDAPSLAV
                     VERFFTKAPELNVGSPSSFVPRDFFDTIRAAPSTIGHYEQP"
     gene            3232..4089
                     /locus_tag="PK710_20495"
     CDS             3232..4089
                     /locus_tag="PK710_20495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12168.1"
                     /translation="MNSPPSLIAQLQEIQQQIRAGSHQEAMARATLASSVTVLSIQPV
                     ATIVLAVRGGIQQAWTRQEEMGEATYQALLAETPPPKTNDRAADYRQIDNEVPVQRIT
                     CTHCRIRPGFGLCPRCQGVGVLLSDRPGTESVLDCPDCEDGFATCTVCGGSTQSRVVT
                     VRYVTIKPLRINHVIPCHGQEALKEAYPDKVDLPSFLAYDLESSSAYRGTSADVGKDY
                     FGFSFEDTIVRAKAFIDRLHSAPSFLVDSLQSFAMPYLLVHLVTRSQIRRRAVFCIDA
                     RQEFFLRGM"
     gene            complement(4169..>4364)
                     /locus_tag="PK710_20500"
     CDS             complement(4169..>4364)
                     /locus_tag="PK710_20500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12169.1"
                     /translation="ILAYDSTTPFAFLGVVDRHSPQAAMHEYLRDEFVRSRSGPGVSA
                     PELLPYPAPITGDEAPLETK"
BASE COUNT          953 a         1358 c         1161 g          892 t
ORIGIN      
        1 caacgactcc aaccattgaa ttcatcgttc cttgcggcga agcgattacc gtctcgctgg
       61 tggtgcaaga tagtcatggt ctgtccagcg cgttggtgca ttccgtggaa gtgaaagaac
      121 aggcaggtcc ctgggtttcc atcaacgatt gtgtggcagg cgatgagtgt ggcacgctgg
      181 atcgtccctg gtgctccatg acggccgcgt atcaacgatg gcctctgatt cgtgcgggag
      241 gagccacggg gttgcgcgtc gttcgaggac gatacgagca aggaaccaag aacaaaaacg
      301 gtgttcccat cgtgtctcca ggcgaaacac tggtgtggaa tgacaccgcg gctaccacgg
      361 acccatcggt agaagcctat gcatccctgg gcggtagtcc aacggcagcc gccagttgtg
      421 ccaagtggga tagcgacccg gaaggtgtag cggtggtgac ccatgagcaa tcgggattcg
      481 tgctcaatga tgatgtcgat gtcgagttgc accgcctttg cttggtagca aaacccacca
      541 gtctcacagc gctgccgatg gtttctgccc tcaccaccgc gggcaatggt tcccggtttg
      601 tggacggcat catctggatg gaccaggttt cggcaccatt gcccgcgcgc aacttcggcg
      661 tttggttctc gagcttcgtt cccaataaca aagtcgcacg tttcgaaaac agtattgttg
      721 tggcgggatc ttccaccgat gaatccgggg gtatgctcat tgggctaaca ccgccaatgc
      781 cgatcttgtt ccaaggtgtc gttcgtatcc aagatgccac cgtgtacttt ggcaacgcgg
      841 catcttccca agccacgtat ggcgtgctgt cgcgcgacga tgtgacggaa gtctattcga
      901 gcacgctggc gggaggcccg ggaaccgacg aaagctacgg tgttttcgcg caagatcagg
      961 ataccttgcg catcgtggga agcgaggtgt tcgggcaagg attggagtcg accaccggcg
     1021 tattcgtcga tgccgtggac aaagttcgta tcgtgggcag cagcctgtac ggatatccaa
     1081 gcgcgtcagc tcccaccacc gtgacgtggg ctaccggtct gcatgcgtac agcggacgcc
     1141 tcatcgatgt gatggcgaat gacgcgtcct atccggatct ggatatcgtg ggcgccgggg
     1201 ccggagtgac cgcaagcacc gcggcacgag gtatttatgt cgaatccctg caagctggcg
     1261 cacaagccgg agagctcacc gtggtcggac ccgacaccgg ctcgacggct cgcattcgtg
     1321 gcggcgattc gctgctgcga tcgtccggaa tcgaaatcga aaccgggatc aagttgacgg
     1381 ttcgtcatct agcaagcatc accgcgggtc gggtgaaaag ctccacctcc ccgttacccg
     1441 tcacccaatt cgtggctgcc gccattcgcc tgcggggtgc cgggggagaa gcaaatattg
     1501 aaaatataaa cgaaattcgt ggatgcgacc ccgcgtgtat cgatcaagga ggtgccatct
     1561 cccaacccga agaaagccct ctattgggta tcggactttt cgccgatgca gatacacaaa
     1621 tctccccacc cacgaaaggt gcctggaata tccttgtaaa aggaccctcg accatccagg
     1681 gagggagcat cgagaaaacc aactcgagca acaaatccgt cgcagaactc atcggaattc
     1741 gcgcattggg cgactacaaa accggtcacc gtttcaaagt ggaacagaat gtggtcgtgc
     1801 ggggctccgt cgccacgagc ccaaacggtc tgccatcgag ttccgcggga gttcgcttgg
     1861 cacgctccag attcattgcc gtcgcccccg tcgaaatcat gggtggcccc gctcaccata
     1921 ccgcccgagg cgtgtggatg aacaatgccc ataccagcgg cacggttctg ccaggcgccg
     1981 gcgcaaccct cgacgtctcg gcgacgtcaa cggattcctt ccgcatcgtc ggcaaccccg
     2041 aatccgccga caaccttcgt cccatggccg tctacggcgt gcaagatgcc tcgaaccgcc
     2101 tggcgatcat agccaatacc gtgagtctga aagcctatct tccgacggcg acggtgccat
     2161 ggtctgcaat cgaaggagga tgggccgcaa tcaattcaca aggagccgcc gtaggggcca
     2221 gcttctccgc aacagccaat gtcgttttgg aacacgttcg catgcatggc ggtgtcgttc
     2281 ccggtggcct cgtacaaagc ggcctcgtca tgaaagacct ttccgctgct cccggtacac
     2341 ggcccgtcgt caacgcatgc ctcatcgaag cttgcggaca agtgcaaggc tcctcccacc
     2401 atcccgattg ccaccagtct ctccaagctt tcagcgtcgg cgccatcatc gatgccaaag
     2461 acgaactcgg atggcccctt ctattctcga acaatctcgt attcggcgga ttcaataccc
     2521 gagcgggagg ctatggaagc atcggaatgg tcgctaccgt cgacaacgac cccatgggag
     2581 cgaccaactc cccagctcgt cacttcatca acaacttctt ttccggacaa ggaacaacac
     2641 ccaccgcttc ttgctccgca ggagtaaacc ccgccgtctc cgaaggtcta aggctcaaaa
     2701 tcggtcccag ctcttctcac aacatccaaa cgactgtcgc cgcatggcgc cggaacatct
     2761 tccacgacgg cggcgtggct tgcaaacgct tcgccgtctc cgagcaagga atcgatgcgg
     2821 acctgaaagg tatccctttc gaatccaacg actacgtcaa agaaatcccc ggtcgtgacg
     2881 tcttccccgt gggctcctac gtggccctct atcgagaagc caagtacctg ggtcccatcg
     2941 accactgcat ctacggcctg ccgtcgctgt tccctagctg catcgacgtc gatatcacca
     3001 acaacactga ctattccaat agcctgaacg tcaatcccct gttcgccacc agcgatgtca
     3061 atgccctgat cgatgcgcca agcctcgctg ttgtcgagag attcttcacg aaggcgccag
     3121 aactcaacgt gggatctcct tcctctttcg ttcctcggga cttctttgac accatccgag
     3181 cggctccctc caccatcggt cactacgaac aaccataaag gcttttggcc gatgaattcc
     3241 cccccttcgc tcatcgccca actccaggag atccaacaac aaattcgcgc gggctctcat
     3301 caggaggcca tggctcgagc caccctggct tcttccgtca ccgtcctgag cattcaaccc
     3361 gttgccacca tcgtgctggc cgtacgcggc ggtatccaac aagcatggac gcgacaagaa
     3421 gaaatgggag aggccacgta tcaggccttg cttgcagaaa ccccaccccc aaaaacgaat
     3481 gaccgcgccg ctgattaccg acaaatcgac aatgaagtac ccgttcaacg gatcacctgc
     3541 actcattgcc gtatccggcc tggcttcggt ctctgcccac gatgccaggg tgtcggtgtg
     3601 ctgctctccg atcgcccagg aaccgaatcc gtcctcgatt gccccgattg tgaagatggt
     3661 tttgctacct gcaccgtctg cggtggatcg acccaatcca gagtcgtcac cgtccgctac
     3721 gttacgatca aacccctgag aatcaaccac gtcatcccct gtcacggtca ggaagcgctg
     3781 aaggaagcct atcccgataa ggtcgatttg ccctcgttcc tcgcctatga cctcgaatca
     3841 tcttccgcct atcgcggtac cagcgccgat gtcggaaaag actatttcgg cttctcgttc
     3901 gaagatacca tcgtacgcgc caaagccttc atcgatcgcc tccactccgc accaagcttc
     3961 cttgtcgatt cccttcaatc gttcgccatg ccctacctgc tcgtgcatct cgtcacgcgt
     4021 tcacaaataa gacgtcgagc cgtcttctgt atcgacgcaa ggcaggaatt tttcctgcgg
     4081 ggcatgtaga aaaacgtgca aattcaatga gatggctgtt agatgacggg gaaggtcgaa
     4141 tgaatcccgt agggagcagc tctgcagttc acttcgtctc caacggggcc tcatcgcctg
     4201 tgatcggcgc tggataaggc agaagctccg gtgcggaaac cccgggaccc gaccgggatc
     4261 gaacgaactc gtcgcgaaga tattcgtgca tcgccgcttg cggcgaatgc cgatccacga
     4321 cccccaaaaa cgcaaacggc gtcgtcgaat cataggcaag gatc
//
LOCUS       DAOSOR010000503         4343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_20266_length_4343_cov_4.703125, whole genome shotgun sequence.
ACCESSION   DAOSOR010000503 DAOSOR010000000
VERSION     DAOSOR010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20266_length_4343_cov_4.703125"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1396
                     /locus_tag="PK710_20505"
     CDS             167..1396
                     /locus_tag="PK710_20505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12170.1"
                     /translation="MNLAVSKLFAPGLASAAMLWTATASAYCPCYTASSSSNTHNCGV
                     EAAPGKNPSIAEWQDIFDLVAQGPSVWGSKGPDVADIGQGCGKPEPTHSVAAKFPCEL
                     LKAIAMAESGWKQFCVPESPSDQVGPPERTIISFDCGYGVGQVTSGMHSGETPAYDRQ
                     RVAADATYNLATGTQILASKWRATKCVGDNQPSTVEHWYTATWAYNGLAWINNPNNPN
                     HDANRGVYNPSVGGSYTYQEKVFGRIEHPPSGGYWPSIPLAYPDRADCGSGGAPPALP
                     EPNCASPTDCSHTRSTHTSTCFPGPLPDGGSDSGQAGASGAAGSAGAGGVELADANEV
                     EPETGPPEASLDSAGDGGWGGQGGSAGKSSSTYHYQAEEPDGCACRAAGNSTCGPWAL
                     VVSLLGAISVLRRRRGS"
     gene            complement(1451..3091)
                     /locus_tag="PK710_20510"
     CDS             complement(1451..3091)
                     /locus_tag="PK710_20510"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT12171.1"
                     /translation="MIPPTRRRNLMLVAAVALIAAGGALLAHRYKKQQDHDAVVAACE
                     ANSATACSQLCNEPNHPSATACLKLGIMYADGTQGVEQDAAKAVPFFSRACEGQELEG
                     CARFGRALLNGAGIERDEPKAVTVLQRACEGHNQLGCVGLGFAKMRGRGTDKDPKGAV
                     ALFQTACDAGQQRGCAELGVAYWNGVGVEKKDEAKAEQLFKTACEANEQRACAGLGVL
                     AMNGPKEKQDPKKAIELFQKACDSDTKDACAFLGSLYVRGMGLERDVPKAIELFKIAC
                     DKAPPAGCAELGELYATGAGVEQNLDEAIRLFKKSCDNHLPEGCVALGRAHLMGLGVE
                     RNPKKAAELFKIACDDNSPAGCTQLGTVEVEGVGIEKNVASGIKRLREACEQEHPPAC
                     TGLATVVETGLDGDKPDYDRALNLYRQACAWGDPNACAGEAAAMMDGRGTKRDVKGGL
                     EKVERACQAGSAQGCSILGTALIMGLGGKSEIERGAKMLGSLCQVGDPASCLKYAAVL
                     AEGRGVKKDIATSRDILDKTCAAGIRPACEMVKRLPKG"
     gene            3238..>4343
                     /locus_tag="PK710_20515"
     CDS             3238..>4343
                     /locus_tag="PK710_20515"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12172.1"
                     /translation="MQKSPFHIPGHKYGRGLPEAFRTRIGNEALLRDLASLVGPEIDS
                     LHDASGCIARAQELIADAFEASRSLMLVNGPDIGLQMAIMAACDPDDEIILSRNVHFS
                     AVGGIVMSGAWPVFTPARFDEELGLLENMTLLDLRHAIQKHPQARALLLMSPSRHGFA
                     PDLDEMVEEAHNAGLTVIVDERLGAHFRFHKKLPISGIEAGADLVVQDWHQCLGALEQ
                     GAVLHVRGDAAERWGRVQNVFQTMTTTSPSYLIMASLDIARHYAVMHGSEALDHCIGL
                     AREIAGSIDRLPGLNVLGYEMLRRSDVDNIDCTRLTITVADTGLTGYRFAQLLEEEHD
                     VVVDMAEESNLILSTGFQLSEEAAYKLIDALERVV"
BASE COUNT          848 a         1188 c         1343 g          964 t
ORIGIN      
        1 tgcttcgtgc cacctccgaa gaccgggcat ggatgacagg gacatgggca atacccggct
       61 ctcttcccgt ggggatcgta gtgcgagcgt cggacggcaa agtcgttacg gtgccgaggg
      121 tagaaggaca tcgggtggag attcttggtt tggcgggagg tgcgtggtga acctcgcggt
      181 ttcgaagctt ttcgcaccag gtcttgccag tgcagccatg ctgtggaccg caaccgcaag
      241 cgcgtattgt ccctgctaca cggcttcgtc gtcgagcaat acgcataact gcggcgtgga
      301 agccgcgccg gggaaaaacc ctagcattgc tgaatggcaa gatattttcg atctggtggc
      361 gcaggggccg tcggtctggg gttcgaaagg ccctgatgta gccgatatcg ggcagggatg
      421 cggcaaaccc gagccgaccc atagcgttgc cgcgaagttt ccttgcgagc ttctcaaagc
      481 catcgcgatg gccgaaagcg gatggaaaca gttttgtgtt ccggaatcac cgtcggatca
      541 agtcggtccc cccgaacgaa cgatcatttc cttcgattgt ggctatggcg tggggcaagt
      601 cacaagcggg atgcacagcg gtgaaacgcc cgcgtacgat cgacaacggg tagccgctga
      661 cgccacgtac aatcttgcga cgggaacgca gattcttgcg agcaaatggc gtgcaaccaa
      721 gtgcgtcggg gataatcaac caagcacggt cgagcattgg tacacggcga cctgggcgta
      781 taatggacta gcgtggatca ataacccgaa caacccgaac cacgatgcga accggggcgt
      841 atacaatcca tccgtaggcg gttcctacac gtatcaggaa aaggtattcg gaaggatcga
      901 gcatccacca agcggtgggt actggccgtc gatccccctt gcgtatccag accgcgccga
      961 ttgcggcagt ggaggtgctc cccccgcgct gccggagccc aattgtgcat cgccgacgga
     1021 ttgttctcat acgaggagca cccacacatc cacgtgtttt cctgggccgt taccggatgg
     1081 ggggagcgat agtgggcaag cgggagcatc gggagccgcg gggtcagcgg gagctggggg
     1141 cgttgagcta gcggatgcga atgaggtgga accggaaaca gggccaccgg aggcatcttt
     1201 ggatagtgcg ggcgatggtg ggtggggcgg ccaaggaggt tcggcgggaa agagttcgtc
     1261 cacgtaccat tatcaagccg aggagccgga cggatgtgcg tgccgggcgg cagggaattc
     1321 gacgtgtggg ccatgggcgc tggtggtttc gctgctggga gccatcagcg tcctaagacg
     1381 ccgcaggggg agttgaaaaa gggcaggcag ggacgagccc ggattcgtca cgcagttttg
     1441 cgttactacg ctatcctttg gggagtcgtt tgaccatttc acacgcgggg cggatgccag
     1501 cggcgcatgt tttgtcgagg atatcgcgtg acgtggcgat gtcttttttg acgcctcttc
     1561 cttcggcgag cacggcggcg tatttgaggc aagaagccgg gtcccccact tggcaaaggc
     1621 taccgagcat tttggcgcct cgttcgattt cggatttgcc accaagtccc atgatgaggg
     1681 cggtaccgag gatggagcag ccttgggccg aacctgcctg gcaggctcgt tctacttttt
     1741 ccagtccccc tttgacatcg cgtttggtgc cgcgcccatc catcatggct gcggcttctc
     1801 cggcacatgc gtttgggtcg ccccaggcgc aagcttggcg atacaggttc agggctcggt
     1861 cgtagtcggg tttatcgcca tcgaggccgg tttccaccac ggtggcaagt cctgtacaag
     1921 cgggggggtg ttcttgttcg caagcttctc gcaggcgttt gattccggag gccacgtttt
     1981 tttctatgcc gacaccttcg acttccacgg taccgagttg ggtacagccg gcgggggaat
     2041 tgtcgtcaca ggcaattttg aatagttccg cggctttttt cggatttcgc tcgactccga
     2101 ggcccatcaa gtgggctcgt cccagcgcga cacatccttc tggcaagtga ttgtcgcagc
     2161 tttttttgaa aagacggatg gcctcatcga ggttctgttc cacgccagcc ccggtggcat
     2221 acaattcccc caattcggcg caaccggcgg ggggggcttt gtcgcaagct attttgaaaa
     2281 gctcgatggc tttgggcacg tcgcgctcca gccccatgcc ccggacatac aaggaaccaa
     2341 ggaaggcgca ggcatctttg gtatcggagt cgcaggcttt ttggaatagt tcgatagctt
     2401 ttttggggtc ttgtttttct ttggggccgt tcatggccaa cacgccaagg cccgcgcaag
     2461 cgcgttgttc gttggcttca caagcggtct taaagagttg ttcggccttg gcctcatcct
     2521 ttttttccac acccaccccg ttccaatagg cgacgccaag ttcggcacaa ccacgttgtt
     2581 ggccggcatc gcaagcggtt tggaataatg cgacagcccc ctttgggtcc ttatcggttc
     2641 ctcttccgcg cattttggcg aatcccaatc ccacgcatcc gagttggttg tggccttcac
     2701 aagcgcgttg caacacggtt acggctttgg gttcatcgcg ttcgatgccg gcgccattga
     2761 gcaaagcgcg tccaaaacgg gcgcatcctt ccaattcctg accttcgcaa gcgcgcgaaa
     2821 aaaaaggcac ggcttttgcg gcgtcctgct ccaccccttg cgtcccgtca gcatacatga
     2881 tccctagttt taggcatgcg gtggcagagg gatgattggg ttcattgcag agttgactgc
     2941 aagcggtggc ggagttggct tcgcaagcgg ccactaccgc atcatgatct tgctgttttt
     3001 tgtagcggtg ggctagcaag gccccgcctg cggctatcaa cgccacagcg gcgactagca
     3061 tcaagttacg gcgacgagtc ggtggaatca ttgggggcat actcgcggat tgtcgcgccg
     3121 ggtcaaggga ggatgcacaa cgggatgttc tttccctggg ttgtcccgta caacagggac
     3181 atgcggcatg atcaatccac gctcccgttg atcgaggcac tcgatgcata catcaagatg
     3241 caaaagtccc cttttcacat ccctgggcat aaatatggcc gtgggttacc ggaagcgttt
     3301 cgtacgcgaa tcggaaacga agctctatta cgggacttgg catcgctggt cggccccgaa
     3361 atcgactctc ttcacgatgc atcggggtgc attgcccgcg cacaagaact gatcgcggat
     3421 gcattcgagg ccagtcgttc cctgatgttg gtcaatggtc ccgatattgg gttgcaaatg
     3481 gcgatcatgg cggcgtgcga cccggacgac gagatcattt tatcgcgcaa cgttcatttt
     3541 tccgcggtgg gtgggatcgt gatgtccggg gcgtggccgg tgttcacccc ggctcgtttc
     3601 gatgaagaac tcggattgct cgagaacatg acgctgctcg atcttcgtca cgcgatccaa
     3661 aaacatcccc aggcgcgtgc gttgcttctg atgagcccat cacgacacgg ttttgctccc
     3721 gatctcgacg aaatggtgga agaggctcat aacgctggat tgactgtcat cgtggatgag
     3781 cggcttggtg cccacttccg cttccacaaa aagcttccca tctccggcat cgaggcaggc
     3841 gcggatctcg tggtccagga ttggcaccaa tgcctcggtg ccctcgaaca aggcgctgta
     3901 ttgcacgttc gtggagatgc ggccgagcga tggggccgcg tgcaaaacgt ctttcaaacc
     3961 atgacgacca ccagcccctc gtacctcatc atggcttcgc tcgacatcgc gcggcactac
     4021 gccgtcatgc atggaagtga agctctcgat cactgtatcg gcctggcgcg agagatcgcg
     4081 ggctcgatcg atcgactccc tggactcaac gtactcggtt atgaaatgct ccgtcgctcc
     4141 gacgtggata acatcgattg cacgcggttg acgatcacgg tcgcggacac agggttgaca
     4201 ggttaccggt tcgcgcagct tctcgaagaa gagcacgatg tggtggtgga tatggcggag
     4261 gagtcgaatc tcattttgtc gactggcttt cagctcagtg aggaagccgc gtataagttg
     4321 atcgatgcgc tggaacgtgt ggt
//
LOCUS       DAOSOR010000504         4327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_20373_length_4327_cov_3.852762, whole genome shotgun sequence.
ACCESSION   DAOSOR010000504 DAOSOR010000000
VERSION     DAOSOR010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20373_length_4327_cov_3.852762"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..275)
                     /locus_tag="PK710_20520"
     CDS             complement(144..275)
                     /locus_tag="PK710_20520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12173.1"
                     /translation="MQLRDATKAAVELLQSRAWGLVRPDDGDMPLDIDPQSSARILP"
     gene            274..2175
                     /locus_tag="PK710_20525"
     CDS             274..2175
                     /locus_tag="PK710_20525"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOT12174.1"
                     /translation="MNGFSLLSFLSFAICVYLGFFALRTDARSKTNRTFAGLCASLGI
                     WAFCYTFIYPEHDDAVRWFWYRLSGIGWTSFAAFALHFFLTISETHRVTSRPWLVALL
                     YVPAVAFLVRLWTGTLLVDSFLTGPMGTVEVQVSGTPWHVAYSLYYLAYMVVGLGIVA
                     WHGRRSASPEQRSQFRIIVVSGTATTLLGSVTNMALPTFGIHVVPAIAPMLILIWLFA
                     IQYSMVRYRLMAPSLEVAVGEIISKIKDLILLTDRRGEITQVNAQVTDLFGISLERLR
                     GRHAMVLAREPEAMRDLLQPVLAGESEGKVSDLDLVVHGEPVPMRIWTAPVRSASNQL
                     LGSVIVAHDLRPKRRLEREVQERTAAEESLRKHNEHLGALHETTLHLIRRHEIKDVLE
                     GLVARATSLVGASSGYIYLTTSDDPECMSLVVATGPMAAHVGHRVRRGEGVAGRVWQD
                     DAPVLIENYRAWHDRLEVFDTHEFRAVAGIPLRTAESTMGMIGVVYEEGDPRRLGEDE
                     LKVLDQFAGLASVALDNARLYQALREELARVEKTTQKLRSARDAAEAASRAKSAFLAT
                     MSHELRTPLNAIIGYADMLEEEARDQQLEGFGHDLLRIKIASQRLLEIIQSVLDYSRI
                     EAGNMEISA"
     gene            2287..2697
                     /locus_tag="PK710_20530"
     CDS             2287..2697
                     /locus_tag="PK710_20530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT12175.1"
                     /translation="MGDPTRVRQSLLNLLGNAAKFTQQGHVKVTTTQEPIDGKPWIVF
                     QIEDDGVGMTKEQVVASFEAFQQVDSSTTRKTGGIGLGLALTRQLCELMGGRLEIQSA
                     VGEGTTVTMRLPILDSHRDFMDSIITEAEVQRTP"
     gene            complement(2682..>4327)
                     /locus_tag="PK710_20535"
     CDS             complement(2682..>4327)
                     /locus_tag="PK710_20535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12176.1"
                     /translation="VGILPFQGADAGSLLQAMTHGPVPSLANAGVDDEHLQRVLNRGL
                     HTSPQHRATSVFDFVAELFDWLSKQAPPRSPSSSRLGAAQALNLVPSGPQRMSPSQND
                     EASARRPYPQPRTATLLYGAQAVQPSLEPAKVEASVPDLEFPLAPDVGSSVEHHPVAA
                     ADDLENSLDTIPPSPSVSSPHCESPKEPQGFSSIPELEAALVRPSRPSSARSASSAPA
                     EDTSSRAVERGQEPRAIPRAKSSESEVEPKPAPSPLPGTHAEPISSNIDENHIVTTTE
                     TNDDPELKSREPVPVVSPSDETSARKKPRRHLAFGMGAAGVLGLLGLTVVAFLYAPKV
                     LSKHFPNSQEPRPSPAPSAMVSNADPWVVPTGSVSSASIEEVEPSEPVARQPGDITRC
                     ITDFFPEGSFDGLQDLGFVCDQPDPRKGALTMRSRVVIGARGFVTPAMHEWSRLEWHE
                     IAVWAIFRHVCCAEPSPLQLPPPVRDCPSLAEAADGLGRAYGAGESLAESIVQFQRAA
                     ECAHVGKASGYRYAAGPISGGQVTFAGFLERNHKRHGVR"
BASE COUNT          932 a         1189 c         1280 g          926 t
ORIGIN      
        1 cgcctcgaaa cacgagccat cgtgaacgga aagaaatgac aaccccgctt tggagtcacg
       61 acgcgtccga atccatcctc gaacggtcac cgacccgtct gtggggatgc gttgcgccag
      121 aatatcactg atgcttatgg ccttcatggc aggatgcgtg ccgaggattg ggggtcgatg
      181 tcaagaggca tgtctccatc atctggacga acgagccccc acgccctact ctgaagcaat
      241 tccaccgctg ccttggtagc atcgcgaagc tgcatgaacg gattctcgct cctttctttt
      301 ctgtcctttg cgatatgtgt gtaccttggt ttttttgcgt tacgaaccga cgcgcgttcg
      361 aagactaacc ggactttcgc ggggctttgt gcgagccttg gaatttgggc gttttgttat
      421 accttcatct atccggagca cgatgacgcc gttcgatggt tctggtatcg cctgtcgggc
      481 attggctgga cttcgttcgc cgcttttgcg ctgcattttt tcctgacgat ttcggaaacc
      541 caccgtgtga cgagtcgacc atggctcgtg gcgctgttgt acgtgcccgc cgtcgccttt
      601 ctggttcgtc tatggaccgg caccttgctt gtcgattcct ttctcaccgg tccgatgggg
      661 acggtcgaag tgcaggtgtc cggcacccca tggcatgtag cgtattcgct ttactacctg
      721 gcttatatgg tggtggggct cgggatcgta gcctggcacg gacggcgttc tgcttcgccg
      781 gagcaacgga gccaattccg catcattgtc gtttccggaa cggctaccac gctgctgggg
      841 agcgtcacca acatggccct tcccacgttc ggcattcatg tcgtgcctgc gattgccccg
      901 atgctcatcc tcatctggct cttcgccatt caatactcga tggttcgata ccggttgatg
      961 gcgccttcgt tggaagtcgc ggtgggggag attatttcga agatcaaaga cctcatcctc
     1021 ttgacggatc gaaggggaga gatcacgcaa gtcaatgcgc aagtcacgga tttatttggt
     1081 atttcactcg aacggcttcg cggacggcat gcgatggtgc tggcccgtga accggaggcg
     1141 atgcgtgatt tgttgcaacc ggtgctcgcg ggtgaatcgg aaggcaaggt aagtgacctg
     1201 gacctcgtcg ttcatggcga gcctgtcccc atgcggatat ggacggcgcc cgtgcgcagt
     1261 gcttcgaatc agctcctcgg ttccgtgatc gtggcgcacg atcttcgacc caagcgcagg
     1321 ctcgaacggg aagtgcagga aagaaccgcg gcggaagaat ccttgcggaa gcataacgaa
     1381 caccttggcg cgctgcatga aacgacattg cacctcattc gcagacacga gatcaaggat
     1441 gtgctcgaag gattggtggc gcgggccacc agcttggtag gtgcttcgag cggatatatt
     1501 tacctgacga cttcggatga tccggaatgc atgtcgctcg tggttgcgac cggtcccatg
     1561 gccgcccatg tgggacatcg ggtccgtcgc ggagaagggg tcgctggcag agtctggcaa
     1621 gatgatgcgc ccgttttgat agagaattat cgagcttggc atgatcgttt ggaggtattc
     1681 gatacccatg aatttcgcgc tgtcgcgggt atccctttac ggacagcaga gtccaccatg
     1741 ggaatgatag gtgtcgtcta cgaggagggg gatccacgaa gattgggtga ggatgaactg
     1801 aaggtgctcg accagttcgc ggggctggct tccgtggctt tggacaatgc gcggctttat
     1861 caagccttgc gcgaagaatt ggcaagagtc gaaaaaacga cccaaaaact ccgatccgca
     1921 agagatgccg cggaagccgc tagccgcgcc aagtccgcct tccttgccac gatgagccac
     1981 gagcttcgca cacctctcaa tgcgattatc ggatacgccg atatgttgga ggaagaagcg
     2041 cgcgaccaac aactcgaagg attcgggcat gacctgttgc ggatcaaaat cgcaagccag
     2101 cgactgctcg aaatcattca aagcgtgctc gattattcac gcatcgaagc tggcaatatg
     2161 gagatctccg cgtaaaaata cgaacccgag tcgattgttc atgaggtcgc gggaatcctc
     2221 gaacccctgc tgcgacaaca ccataacgct ctcaccattc atatcccatc gcccatcggt
     2281 agcatcatcg gagaccccac acgcgtgcgt cagtcccttc tgaaccttct tggcaacgcc
     2341 gcgaagttca cccagcaagg acacgtcaaa gtaacgacta cgcaagaacc gatcgacggc
     2401 aaaccgtgga ttgtcttcca gatcgaggat gatggcgtgg ggatgacgaa ggaacaagtc
     2461 gtggcatcct tcgaagcatt tcaacaagta gactcctcga ccacccgcaa gacgggaggc
     2521 attggtcttg gtctcgcact gaccagacaa ctgtgtgaac tcatgggcgg aaggctcgaa
     2581 atccaaagcg ccgtggggga aggaaccacg gttacgatgc ggttgcccat cctggattcc
     2641 catcgcgact tcatggattc gatcatcacc gaggcggaag ttcaacgtac gccgtgacgc
     2701 ttgtggttgc gttcaagaaa gcccgcgaac gtcacctgac ccccggagat ggggcctgct
     2761 gcatatcggt atccggaagc ctttcccaca tgcgcgcatt ccgcggcccg ttgaaactga
     2821 acgatggact cggcaagcga ctcacccgca ccataggctc gaccaagccc atcagccgcc
     2881 tcggccaaag agggacaatc tcgtacggga ggtggaagtt gaagcggtga aggctccgcg
     2941 cagcagacgt gacgaaatat cgcccaaacc gcaatttcat gccactcgag acgagaccac
     3001 tcatgcatgg ccggggtcac gaaccctcgg gctccaatca ccaccctgga ccgcatcgtt
     3061 agtgcgcctt ttcgagggtc cggttgatcg catacgaaac ccaaatcctg caaaccgtcg
     3121 aaagaaccct ccgggaaaaa atccgtaata caacgtgtga tgtctccagg ctgtctcgcg
     3181 acaggttcgg atggttcgac ctcttcgatc gaagctgacg atacggatcc tgtgggaacc
     3241 acccaagggt cggcattcga aaccatggcc gagggggcag gcgatgggcg gggctcttgg
     3301 gaattgggga agtgtttcga caaaaccttg ggagcataga ggaaggccac aaccgtgaga
     3361 cctagcagcc cgagtacacc cgcagctccc atgccaaacg ccaggtgccg tcttggtttt
     3421 ttccgcgcag aagtttcatc cgaaggcgaa acgaccggca ccggctcacg ggatttcaat
     3481 tcaggatcgt cattggtctc tgtcgttgta actatatgat tttcatcaat atttgatgag
     3541 atcggttcgg cgtgcgtccc tggtagggga ctaggagctg gtttgggttc aacttctgac
     3601 tccgagcttt ttgctcgtgg gattgcgcga ggttcttgac ccctttctac cgcccgactg
     3661 ctcgtgtctt cagcaggtgc agaggaagcg gaacgcgccg acgaagggcg agagggcctg
     3721 acgagcgcgg cttccagttc gggaatagag gagaaacctt gtggctcttt cggactttcg
     3781 cagtgcgggg aagaaacgga cggcgacggt gggatggtat cgagggaatt ttccagatcg
     3841 tctgccgccg cgacaggatg atgctcgacc gaagagccaa cgtctggagc cagggggaat
     3901 tccaggtcgg gtactgaagc ttcgaccttt gccggttcga gcgacggctg cacggcttgc
     3961 gcgccataga gcaacgttgc cgttcgtggt tgaggataag gacggcgagc cgaggcctcg
     4021 tcgttctgcg atggagacat tcgttggggg ccagaaggga ccaagttcaa cgcttgcgca
     4081 gcaccgagcc gcgaggaaga cgggcttcgt gggggagcct gtttgctaag ccaatcgaat
     4141 agctctgcca cgaaatcgaa gacgctggtc gctcgatgct gcggcgacgt atgcagcccg
     4201 cgattcaata cgcgctgaag atgctcgtcg tcgacaccag cgtttgccaa cgagggcaca
     4261 ggcccgtggg tcatggcttg taacaaagac ccagcatccg cgccttgaaa tggcagaatg
     4321 cccacca
//
LOCUS       DAOSOR010000505         4321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_20409_length_4321_cov_3.896156, whole genome shotgun sequence.
ACCESSION   DAOSOR010000505 DAOSOR010000000
VERSION     DAOSOR010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20409_length_4321_cov_3.896156"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3418
                     /locus_tag="PK710_20540"
     CDS             <1..3418
                     /locus_tag="PK710_20540"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOT12177.1"
                     /translation="NTEGAPVDAAQRLVRDWVDRIGVRGQVRGFGVTGSGREVVGSLL
                     RTCYGNDRVFVMNEIAAHAEGALSIDEKVDTIFEIGGQDAKYIRLEGGRVIDAAMNEA
                     CSAGTGSFIAEQGAKFEGVGDDVVRLGQLAEGADFGISLGQHCSVFMAEVIDEAITQG
                     VERDAIIAGLYDSIIQNYLNRVKGTRSVGQRIFCQGMPFSSPALAAAVVRQTGREVVV
                     PPNPGTIGALGIALLTRKEMVSAQTVAEEALDPHRFMGAEVVSKETFICKSTKGCGGS
                     GNLCRIDRLTTRVSGLKQKFLWGGNCSLYDRGAGRKKLPDLAPDPFREREELVDAILR
                     DCRRDPGSAQVIAMTDEFSLKGMLPLFVTFLHNLGFDCQVLRNADGKALRRGIEGARV
                     PYCAPMQMAHGVFFELAKKQPDYAFIPMLQELPSVKGEVHSTVCPVVQAAPDVIGSLI
                     EGDLSKRRILRPILRFGGEGYRGRQFTESLRRFAEQLGASHRFAEAFKKAVHAQETFE
                     ADCVKIGERALRFAEKHQVVPVAVLGRPYTIYNDVLNSNVPNLLRALGALAIPVDCLP
                     VPDDIPRFNDQYWGYTQRNLRAATFVRQTPGLYSVFCSNYACGPDSFTLQFFSYLMQG
                     KPFAIVETDGHSGDAGTKTRMEAFLYCVDSDVRSSASEHNKINDLKALEEVKAPADRV
                     KADQSLLLIPRMGTGAEVAAACLRSEGFRCEHLPMPDREDVRTGRRYTSGKECVPMIF
                     TTGALLNRLERDRHTQEKFTLAMPTASGPCRFGVYNVMHKVILEQTGWSDRVNVFSPD
                     DGDYFRETSPEFMVKLWCGFVAYDMLEAMLYDVRPVERRKGATNEIYRKYKEELIAGM
                     EKPSKGTALNATLEVLSGMWGMRDLLTRAAQDMAAAKNRVRDVPSVAVVGEIYVRLDP
                     FANDFIIDKLEERGIRARFAPFVEWLEYTQYLAEKRVLDKAMRHDDNPLSIGVTGVVQ
                     RASLQVLYDICAKALQWPERTTVNSTLDAALPYLNTELTGEACLTLGGPIHEFNHKLV
                     QGVVVVGPHECMPCRIAEAQYGKVAEDMNLPYLVLALNGDPLDTEALDRFAYDIHESH
                     RRGLGRDLGTVFKNMGVDNRLSITADDPTLVPLRLSADDRMMSHGRRAQD"
     gene            3641..>4321
                     /locus_tag="PK710_20545"
     CDS             3641..>4321
                     /locus_tag="PK710_20545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12178.1"
                     /translation="MNNRLLWVGFFLGAWGMGACSSAGSTDKDYGGGGSLGGEDGGAG
                     SGGSGIDVPDSGFLEVSTEDTALETGGTKEPIPVDCTGCQGIGEGLENLLCAIELCDP
                     KMVLNSEYHSPTATKPQKIASSRSAVARFGAPNNGLAPLVNDSYALMATGPAKGTDHN
                     VTLGDFGLTTPDGASVPDPFAPPQDTINLTYDVMEWKLRLKAPAEAHGFRINYVFFSE
                     EYDEYIGRE"
BASE COUNT          911 a         1091 c         1368 g          951 t
ORIGIN      
        1 caatacggaa ggagcgcccg tggatgcagc gcagcgtctc gtccgtgatt gggtcgatcg
       61 cattggagtg cgaggacagg tgcgtggatt cggcgtaacc ggatcgggac gagaggtcgt
      121 cgggtctttg cttcgaacgt gttatggcaa cgatcgcgtg ttcgtgatga atgagatcgc
      181 ggcccacgcg gaaggtgcct tatccatcga tgaaaaggtc gacaccattt tcgagattgg
      241 tggacaagac gcgaagtaca ttcgtctcga agggggccgt gtgatcgacg cggcgatgaa
      301 cgaagcgtgt agcgccggca cgggatcatt cattgcggag cagggggcca agttcgaggg
      361 tgtgggcgac gatgtggtgc gtcttggtca attggcggag ggtgcggatt ttggcatctc
      421 gttgggtcag cattgttcgg tgttcatggc ggaggtgatt gacgaagcga ttacacaagg
      481 ggtggaaagg gacgcgatca tcgccgggct gtacgattcg attattcaga attatctgaa
      541 tcgtgtgaag ggtacgcgat cggttggcca gcgaattttc tgtcagggaa tgccgttcag
      601 ttctcccgcg ctggctgcgg cggtcgttcg tcagacgggt cgtgaggtgg tggtgccgcc
      661 gaatcccggc acgatcgggg ctttgggaat tgctcttctc acccgaaagg aaatggtttc
      721 agcgcaaacc gtggcggaag aggctttgga tcctcatcga ttcatgggag ccgaagtcgt
      781 ttccaaggaa acattcattt gcaagtccac caaaggctgt ggaggaagcg ggaatctgtg
      841 tcggattgac cggctgacca cacgagtcag tggtctgaaa cagaagttcc tgtggggagg
      901 caactgctcg ttatacgacc gtggcgcagg gcgaaaaaag cttccagatc tcgccccgga
      961 cccattccgc gaacgggaag agttggtgga tgcgatcttg cgcgattgtc gtcgggaccc
     1021 tggcagtgcg caggtcatcg cgatgacgga tgaattctcg ctcaagggga tgctgccgtt
     1081 attcgttacc ttcctgcaca acctgggatt tgactgtcag gtattgcgca atgcggatgg
     1141 caaggcattg cgtcgtggaa tcgagggggc gcgggttccg tattgtgcac cgatgcaaat
     1201 ggcgcacggc gtattcttcg aactcgcgaa aaaacagcct gattatgctt tcattccgat
     1261 gctccaggag ttgccatcgg tcaaaggaga ggttcactcc acggtctgtc cggtcgtgca
     1321 ggcggccccg gacgtcattg ggagcctcat cgaaggggat ttgagcaagc gtcgaattct
     1381 tcgtccgatc cttcggtttg ggggggaagg ctatcgtggc cggcaattca ccgagtcctt
     1441 gcgtcgtttc gcggagcaat tgggcgcgag ccatcgattc gcggaagctt tcaagaaagc
     1501 agttcacgcg caggaaacct tcgaagccga ttgcgtgaag atcggggagc gcgctcttcg
     1561 attcgcggag aaacatcaag tcgtcccggt ggccgtgctc ggacgtccct acaccattta
     1621 caacgatgta ttgaattcga acgtccccaa cttgctgcgt gcgctcggag cgttggccat
     1681 cccggtggat tgtctgccag tgccggacga catccctcga ttcaacgatc aatattgggg
     1741 ctatacgcaa cgcaacttgc gggcagccac gttcgttcgt caaacccccg gtctatattc
     1801 cgtgttctgc agcaactacg cctgtggtcc cgacagtttc acgctccaat ttttcagcta
     1861 tctgatgcag ggcaaaccct ttgccatcgt ggagaccgac ggtcattctg gcgatgcggg
     1921 gaccaagaca cgcatggaag cgtttttgta ttgcgtggat tcggacgttc gctccagcgc
     1981 gtcagaacac aataaaatca atgacttgaa ggcattggag gaggtcaagg cgccggcgga
     2041 ccgtgtgaag gcggatcaat cgttgctcct cattccgcga atgggaacgg gtgcggaagt
     2101 tgccgcggcc tgtcttcgca gcgaggggtt ccgttgtgag cacttgccca tgccggatcg
     2161 agaagacgtg cgcacgggtc gtcgatacac ctctggaaaa gagtgtgttc cgatgatttt
     2221 taccacgggt gcgctgctca atcgtctgga gcgggatcgt cacacgcaag agaagttcac
     2281 gcttgccatg cctacggcct ccggtccttg tcgtttcggc gtttataatg tcatgcacaa
     2341 ggtgattttg gagcagacgg gatggagtga tcgcgtcaac gttttctctc cggacgacgg
     2401 cgattatttc cgggaaacgt cgccggagtt catggtcaag ctttggtgtg ggttcgtggc
     2461 ttacgacatg ctcgaggcca tgctttacga tgttcgtccg gtggagaggc gaaagggcgc
     2521 aaccaacgaa atctaccgaa aatacaagga agaactcatt gcaggcatgg aaaaaccctc
     2581 gaagggcacg gcgttgaacg cgaccctgga ggtattgagc gggatgtggg ggatgcgcga
     2641 tctattgacc cgcgccgcgc aagacatggc ggccgccaag aatcgcgttc gagatgtgcc
     2701 ttctgtcgcg gtcgtcggtg aaatctacgt tcgtctcgac cccttcgcca acgacttcat
     2761 catcgacaag ctagaggaac gcgggattcg ggcaaggttc gcaccttttg tcgaatggct
     2821 cgagtatacg caatatctcg cggaaaaacg cgtgttggac aaagccatgc ggcatgatga
     2881 caacccgttg tccatcggtg tgacgggggt cgttcaacgc gcttcgctgc aggtgctcta
     2941 cgatatctgc gccaaagcgc ttcagtggcc ggagcgcacc acggtcaact ccacgctcga
     3001 tgccgccctt ccctatctca atacggagtt gacgggagaa gcgtgtttga cccttggagg
     3061 tccgatccat gagttcaacc ataagctcgt gcaaggcgtg gtggtggtgg gacctcatga
     3121 gtgcatgccc tgtcgaatcg cggaggctca atacggcaag gtggcagaag acatgaacct
     3181 tccgtatctc gtgctcgctc tcaatggcga cccgctcgat accgaagccc tggaccgatt
     3241 cgcctacgac attcatgaat cccatcgtcg tggcctgggt cgggacctgg gcacggtctt
     3301 caagaatatg ggagtggaca atcggctatc gatcacggcc gacgatccca cgctcgttcc
     3361 gcttcgcttg tccgcggacg atcggatgat gagccacggg cgaagagcgc aagactgatc
     3421 gccgcatcct aaaatatcta taaataacaa ttacttacac cacgcccgaa accctgtccg
     3481 cagccctggg gcaaggcttg cagaccgctt gctttcgggt gtcgtcgatg ccgggcgata
     3541 gcaggttcat cgctgggtca ggtatggcgg tgtgggggta taaggagcga ggttcgaaca
     3601 caaacttgga caattccgaa gttattgtca taaccaaacc atgaacaacc ggttgctatg
     3661 ggttggtttt ttcttggggg catggggtat gggggcctgt tcctcggccg gaagcaccga
     3721 taaggattat gggggaggcg gaagtctcgg tggtgaggac ggcggtgctg gcagcggagg
     3781 cagtggcatc gacgttccgg attcaggttt tttggaggtg agtacggaag acacggcgct
     3841 tgaaacggga ggaacgaagg agccgatccc cgtggattgt acgggttgtc aagggattgg
     3901 ggaggggttg gaaaacttgt tgtgcgccat cgaactctgt gatcccaaga tggttttgaa
     3961 ttcggaatac cattccccga ccgcgaccaa gccgcaaaaa atagcaagtt cgcgttcggc
     4021 ggtggctcgg ttcggcgctc ccaacaatgg cctggcgccg ctggtcaatg attcgtatgc
     4081 gttgatggcg acggggccgg cgaaagggac ggaccacaat gtgacgctgg gcgactttgg
     4141 cctcaccacg cccgacggag ccagtgtacc ggacccattt gcgcctcccc aagacacgat
     4201 caacctgacg tacgatgtga tggagtggaa gttgcgactc aaggcgccgg ccgaggcaca
     4261 cggctttcgc atcaactacg tctttttctc tgaagaatac gacgaataca tcgggcggga
     4321 g
//
LOCUS       DAOSOR010000506         4320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_20412_length_4320_cov_3.727550, whole genome shotgun sequence.
ACCESSION   DAOSOR010000506 DAOSOR010000000
VERSION     DAOSOR010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20412_length_4320_cov_3.727550"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PK710_20550"
     CDS             <1..247
                     /locus_tag="PK710_20550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12179.1"
                     /translation="CWPPWTTPSIVNTRVVSRCSTRTIPRIPFACASTPAGSSEDGVG
                     EGIEDEAEDEAERKRVGERSQPAETIKIIIKINTIKQ"
     gene            508..2955
                     /gene="dacB"
                     /locus_tag="PK710_20555"
     CDS             508..2955
                     /gene="dacB"
                     /locus_tag="PK710_20555"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOT12180.1"
                     /translation="MITVSRMSFLTLGLVALMVAACGDDDDSSSWSWGDAGGSPSAGG
                     TGGWAGSAGGAGIAGEAGASGEAGSSPQGGSGGSGGSGGSGGAGATGGSSGSGGTSQG
                     GSGGSPPAGDPFEPPPSPPSIPTAKRESIANSVDTILASGTVASASHSILIVDPDTNT
                     TLFERNPNTVLKPASNTKLFTSATAFGQLGQDHRFASGIYADAPISPTGTVSGKLYLV
                     GQHDFTWSTFFYDDPRFALDRIAADLYALGLRTVTGNVEVRGEFVYDGYQFDAYDPAA
                     HRTTVANRFRSALIAKGISVGSATTNSSMAVPASGTLLTEWHALPLSVAASPINRTSH
                     NEFADILLRHVGWMQNSTSSYDSGANVVLSWLLTLGMDTSGIAMHDGSGLSHDNRFSA
                     QHVVSLLQTMAKRPEGQAWERTFSVAGVRGTLSSRMTGADTVGRFFGKTGTLNGVIAT
                     SGYLDHRHLGRRLLFGMLMNNVANQTNARTAHDKIIQAYAADLLSTGSRPSAPELESV
                     TNDGNGATVTIRWTPATGASGYLVWSSADAMLWKRELARRVTGTSHVFGGLTPNERVY
                     IRVSAFNDSGESDPSDVYGTKATKTASTILIVDGNDRWQRQPAPENTTGGGHDFVALV
                     AEAIETQGYDTVTHEAVIQGQVPLKNYGAVLWHLGEEGSEHRTFELAEQQAITAYLQQ
                     GGSLFVSGAEIGYDLMTSGIAADQTFLQEVLRAQYLADDAETFVVKGSGGILQNVGRL
                     GFYTPDRMVVSYPDQIAPTGGSKAILSYDGGLGGTAGIQYEGAYRLVYFGFPFESIDA
                     REERRAVMSRVLGFLLP"
     gene            3147..>4320
                     /locus_tag="PK710_20560"
     CDS             3147..>4320
                     /locus_tag="PK710_20560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12181.1"
                     /translation="MNHLTPTHLARLSVFLVVIVAFACGGDDADELYQPNPPKTTCKT
                     SSDCAAPSAVCDVENGTCVQCLNDTHCDGGARCISNRCVAPTGCTSSLDCVNVPHAPI
                     CDPSSQNCVPCVGPEDCSGTADCIKNQCVPYHACQTSLDCPPNQVCASLLGRCVECET
                     DFDCPTDHVCLGNSCEVRTPCVSDTQCTPQGKLCDKSLGYCVECMTNQQCHEGHHCAQ
                     GVCRPDECDAGESTCKSNALLMCDPQGHRWMDPVACGSQTTCVVKGKSASCEPWICMA
                     GTTACDGDKVVECSKDGMTIISSVDCSVLKKHCLDGECSDLACTPNSKYCVGAELRQC
                     NAAGSDYSVLTVCEPKQVCNSTAGACVNKLCEPGQPACDGERATKYNAAGDGYESGG"
BASE COUNT          941 a         1200 c         1259 g          920 t
ORIGIN      
        1 ctgttggccg ccctggacga ccccatcgat cgtcaacacc cgcgtggtat cccgctgctc
       61 gacccgcacg attccccgaa tccctttcgc ttgtgcgagc acaccggcag gtagctcgga
      121 agatggcgta ggggaaggga tagaagatga ggcggaggac gaggccgagc gaaagcgcgt
      181 gggcgagcgt tcgcaacccg cggaaaccat aaaaatcatt ataaaaatca atacgataaa
      241 acagtaacgg gggaacatgg gggcatggaa acacagcgac tgctttggtt caactccttg
      301 cgtgtggtcg gggccgaatt ctgctggatt cgatgaatgc cctgctcctg catggggtcc
      361 tgttttcaat gcataccaga ttccgcggaa tttatggtct atcggagggt tcgagatcaa
      421 cgttatgggc ccggttctgt catttgtctc gtgaagaggc accaacgcat gggccatggc
      481 atgaaaatgc gctatactga gtaccccatg ataactgtct ctcggatgag ttttcttacc
      541 cttggtcttg ttgccctgat ggtggccgct tgtggcgacg acgatgattc ttcgtcgtgg
      601 tcctgggggg atgcgggggg aagtccttcc gcgggtggca ccgggggttg ggcgggaagt
      661 gctggaggcg cggggatagc cggcgaagct ggagcgtctg gcgaggcggg ctcatcaccc
      721 cagggcggct cgggtggttc gggaggttca ggaggcagcg gtggtgcagg cgccactgga
      781 ggaagcagcg gttccggggg aacgagtcaa ggcggctccg gaggtagtcc ccctgcggga
      841 gatcccttcg agcccccccc ttccccaccg tccataccca ccgcaaaacg cgagagcatc
      901 gctaattccg tcgataccat cctcgcctcg ggaaccgtcg catccgcctc ccattccatt
      961 ctcattgtcg atccggacac caacactacc ttattcgagc gaaatccgaa cacggttctc
     1021 aagccagcgt ccaacaccaa gctattcacc tcggccaccg ctttcggcca gcttgggcag
     1081 gaccatcgtt ttgcctcagg catttatgcc gatgctccca tctcgcctac cggaacggtc
     1141 tcgggaaagc tatatcttgt tggacagcat gacttcacct ggtccacctt cttttacgat
     1201 gacccccgat ttgctctcga ccgtatcgcg gccgatttat acgcgcttgg gttgcgcacg
     1261 gtaacgggta atgtggaagt gcgcggcgag ttcgtttacg acggatatca attcgatgct
     1321 tatgatccgg cggctcatcg cacaaccgtc gccaaccgct ttcgttccgc gcttatcgcc
     1381 aaggggatca gtgttggttc ggcgacgacg aactcatcga tggcagttcc tgcatcggga
     1441 acattgctta cggaatggca cgcgctaccg ctttcggtgg cggcttcccc catcaaccgc
     1501 accagccata atgaattcgc cgacatactg ctgcgccacg tcggttggat gcagaattcg
     1561 acatcctcgt acgatagtgg agccaatgtt gtcctttcct ggctgctcac gttggggatg
     1621 gataccagtg ggatcgcgat gcatgacggc tcgggcctat cgcacgacaa ccgcttttcg
     1681 gcgcagcacg tagtgtcttt gctgcaaacc atggcaaagc gtcccgaggg acaggcttgg
     1741 gaaagaacct tcagtgtcgc gggcgttcgc ggcacgctgt cttcgcgcat gacaggagcg
     1801 gatacagtcg gccggttttt tggcaaaacc ggtactctca acggagtcat cgctaccagc
     1861 gggtacctcg accaccgcca tctcggacgc cggctccttt ttggaatgct catgaacaat
     1921 gtcgcaaacc aaaccaacgc gcgcaccgca cacgataaaa tcattcaagc ttacgccgcg
     1981 gacctgctct cgacgggctc ccgtccttcc gctcctgaat tggaatcggt caccaacgat
     2041 ggcaatggcg caaccgtcac gatccgctgg acccctgcca cgggcgcatc ggggtatctc
     2101 gtctggtcga gcgccgacgc catgctatgg aagcgcgaac tggcacgtcg tgtcaccgga
     2161 acctcccacg ttttcggggg actaaccccc aatgaacgag tgtatatccg ggttagcgca
     2221 ttcaatgatt ccggcgagag cgacccatcg gatgtgtatg gaaccaaagc caccaagacc
     2281 gcgtcgacca tcttgatcgt cgacggaaat gatcggtggc aacgtcagcc ggctccggaa
     2341 aacaccacgg gtggagggca tgatttcgtc gccctcgtgg cagaagcgat tgagacccaa
     2401 ggatatgaca cggtcaccca cgaagcggtg atacaaggac aagttccgct gaaaaattac
     2461 ggtgcagtgc tttggcatct tggagaagaa ggcagtgaac acagaacgtt cgagctggcc
     2521 gaacaacagg cgatcaccgc ctatctgcag caaggcggaa gtcttttcgt cagcggcgcc
     2581 gagattggct atgacctaat gacctcgggg atcgcggcgg atcaaacgtt tcttcaagag
     2641 gttcttcggg cgcaatacct tgcggacgac gccgaaacgt tcgtggtcaa gggaagtggt
     2701 ggtattttgc agaatgtcgg aagactcggt ttttatacac cagaccgcat ggtcgtatcc
     2761 tatccggacc aaatcgcgcc caccggcggg agcaaagcga tcctgtcgta tgatgggggc
     2821 ctcggaggca cggccggcat tcaatatgaa ggggcctatc gcttggtgta tttcggcttt
     2881 cctttcgaat ccattgatgc ccgagaggag cgacgcgccg tcatgagccg cgtattggga
     2941 tttctcctcc cgtgacgcct tcggcgccca tgggcctgga aacctcatcc tgaaatcaaa
     3001 aataacggat actatagagg actaagagaa ccgtgcaaga cagaggattg cccatcgcca
     3061 atcggaagac aaaccccagg gaatacctaa cgaacaacaa gcgtattacg caaggcgccc
     3121 cttgatgaag cgagcttgag gagttcatga accacctcac cccaacccat ttggcaagat
     3181 tatccgtatt tctagtcgtc atcgtcgctt tcgcctgcgg gggagacgat gcggatgagt
     3241 tgtatcagcc caatcctccg aaaaccactt gcaaaacatc gagtgattgt gccgcgcctt
     3301 ccgccgtttg cgatgtggaa aacggcacct gcgtacaatg cctcaacgac acgcattgcg
     3361 atggcggggc tcggtgtatt tcgaaccgct gcgtagcgcc cacgggatgc accagcagtc
     3421 tggattgtgt gaacgtgccc catgctccca tttgtgatcc ttccagccaa aactgtgtgc
     3481 cctgtgtggg accggaggat tgttcgggaa ccgcagattg catcaagaac caatgtgttc
     3541 cttaccatgc ctgtcaaaca tcgttggact gcccccccaa tcaggtatgt gcttcgctgt
     3601 tagggcgttg tgtcgaatgc gaaacggact tcgactgtcc cacagatcat gtgtgtttgg
     3661 gcaactcctg cgaagtccga accccttgcg tatcggatac tcagtgtacg ccgcagggaa
     3721 aactatgcga caagagtctt ggttattgcg tggaatgcat gacgaaccaa caatgccatg
     3781 agggccacca ttgtgcgcag ggggtctgca ggccggatga gtgcgatgcg ggcgaatcaa
     3841 cctgcaagtc caatgctctt ttgatgtgcg acccgcaagg tcaccgctgg atggatcccg
     3901 ttgcgtgcgg ttctcagaca acgtgtgtcg tgaaagggaa gtcagcgagt tgcgagccct
     3961 ggatctgcat ggcgggcaca acggcatgcg atggtgacaa ggtggtggaa tgttccaagg
     4021 acgggatgac tattatttcc tcggtcgatt gctccgtatt gaagaagcat tgcctcgatg
     4081 gtgagtgcag tgatctcgct tgcacgccca attcgaagta ttgtgtaggt gccgaactgc
     4141 gccaatgcaa tgcggcaggc agcgattatt ccgtactcac cgtctgtgaa cctaagcagg
     4201 tatgcaattc gacggcgggc gcgtgtgtca acaagctatg tgaaccgggc cagccggctt
     4261 gcgatggtga acgagccacg aaatacaatg cggcggggga tggctacgag tctgggggaa
//
LOCUS       DAOSOR010000507         4298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_20538_length_4298_cov_4.018855, whole genome shotgun sequence.
ACCESSION   DAOSOR010000507 DAOSOR010000000
VERSION     DAOSOR010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20538_length_4298_cov_4.018855"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PK710_20565"
     CDS             <1..185
                     /locus_tag="PK710_20565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12182.1"
                     /translation="NDGGQRWTREYPTLEQVQPGQQAARMGINAYEHTEKYTYFRADM
                     SDSYESTKLEQQRGWV"
     gene            189..467
                     /locus_tag="PK710_20570"
     CDS             189..467
                     /locus_tag="PK710_20570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12183.1"
                     /translation="MVWLPNLPGTSHPSFFFHDEVHKTADRAGLTTRILLHTAQRPEP
                     AGGEPASPCHWKVEDRTYRIANGGGVATVSTLLPPKADVFEVGGFDGA"
     gene            570..1115
                     /locus_tag="PK710_20575"
     CDS             570..1115
                     /locus_tag="PK710_20575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12184.1"
                     /translation="MDIGTWRIEVSATPPAQHEEYLHFVAVTDDGQTPITAQQIKANS
                     GTAARVGNLTVLFAVDGATEITVQAPGEQRLLVVGLEPSGRYDVTLSGSDTILTKTAN
                     GPQVASSQGVLYLSRGLDPDGDAGGLVSDGGDSPGAGKSSDGCSCGLIRGSAMSSQAA
                     WLASVFFGLLAVVRRRGGAVS"
     gene            complement(1384..2076)
                     /locus_tag="PK710_20580"
     CDS             complement(1384..2076)
                     /locus_tag="PK710_20580"
                     /inference="COORDINATES: protein motif:HMM:NF019464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HOT12185.1"
                     /translation="MALTLGRDDEVIQKVDETWARLERLERQFAPLLELKEDAQPILK
                     ELMHAVILELDELDGHFKLEDALLLIKRLLRNTKNLSALLDRLEQANDLISDMTPIIH
                     EVTKATISELGELEKQGIFKNLAAIRTVAERFSRRYTPERVVALGDKLIELLQLVDLI
                     AQPRTLRLAHAATDAISQDDQGRAEVTMFGLWRKMRDPDTLRGLALMLDLAKALGQAD
                     NTKSVPMLESGK"
     gene            complement(2086..2553)
                     /locus_tag="PK710_20585"
     CDS             complement(2086..2553)
                     /locus_tag="PK710_20585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12186.1"
                     /translation="MIEDCNLDGGNAGYLPTDKATLKAKDLENVYVIGDNTDLPTSKA
                     GSVAHFASEILCENLLREIEGLEPRAVFDGHSNCFIESGFEKGILIDFNYDVEPLPGR
                     FPLPGVGPFALLQETRMNHWGKLMFKWIYWNILLKGEEMPLEPQMSMFGKWAD"
     gene            complement(2550..3209)
                     /locus_tag="PK710_20590"
     CDS             complement(2550..3209)
                     /locus_tag="PK710_20590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HOT12187.1"
                     /translation="MHHYQPGYLFIPFGMYTKEMVLKPKRDFIPPGVEFVLDEVTEID
                     PKNKRVGTKKGRKLNYDFLVVALGCRIAPEEVEGMMEGWGKNVHTFYSLDGAVALAKA
                     LKFFDKGRLVLNIAEIPFKCPVAPLEFVFLADWFFHERGVRDKVEIELVTPLPHAFTK
                     PKAAAILGDFCERKNIIITPNFNIGEVNAAEGYIEELGAEGRKVNFDLLVAIPPNLGP
                     R"
     gene            complement(3665..>4298)
                     /locus_tag="PK710_20595"
     CDS             complement(3665..>4298)
                     /locus_tag="PK710_20595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12188.1"
                     /translation="FRFALKEGCPYISYARLKHSGQFKTGPSKEWVDIKGEQYFTTQI
                     PGFVWKGSTTMFTARDMYLSNRGKLTVSLLSLFGIVDGQGEIYDQGELLRWLAESVWF
                     PTNLLPGDNLQWVPMDGTSARLNFTYNNICISYLVSFDDFGAITEFKTKRYMNENDLE
                     IWVGKVTDYREKAGMMIPFAIEALYQLKDGDYSYAKFYLNEIEYDVPEKY"
BASE COUNT          878 a         1206 c         1227 g          987 t
ORIGIN      
        1 tcaacgacgg ggggcagcgc tggacacggg agtatcccac gctcgagcag gtccaacctg
       61 gacaacaggc cgcccgcatg gggatcaatg cttatgaaca taccgaaaag tacacgtatt
      121 ttcgggctga tatgagcgat agctacgaat cgacgaaact cgaacagcag cgaggatggg
      181 tgtgaagcat ggtttggtta ccgaatctac caggcacttc ccatccatcg tttttctttc
      241 acgatgaggt ccataagacc gcggacagag caggattgac aacgagaatc cttcttcaca
      301 cggcacaacg accagaaccg gctggcggtg agcctgcctc gccttgccat tggaaagttg
      361 aggatcggac ctatcgaatc gccaatgggg gaggtgttgc gacggtgagt acgcttcttc
      421 ctcccaaggc cgatgttttc gaagtgggag gctttgatgg cgcgtaagac tgccgattcc
      481 tcgcttggag cccggatggc aaggaaacga actatcctcc gaacaaggat cgggatccgc
      541 attacgaccc ggagaacaat accaatgcag tggatatcgg cacctggcgt atcgaggtgt
      601 cggcaacgcc acctgcgcag catgaagagt atttgcactt cgtggcagtg accgatgacg
      661 gacagactcc aattaccgcg cagcagatca aggcaaatag cggtacggct gcacgggtcg
      721 gcaacctgac cgtgcttttt gccgtcgatg gcgcaacgga aatcactgtg caagcacctg
      781 gggaacagcg cctgctcgtg gtcggcctcg aaccttcggg ccggtacgat gttacattat
      841 caggaagtga tacgatattg acgaaaacgg cgaatggtcc gcaagtagcc tcttcgcaag
      901 gggtattgta cctttcgcgg gggctcgatc ccgacggcga tgctgggggg ctggtgtctg
      961 atggcggaga ttcccctgga gcgggaaagt cttccgatgg ttgttcctgt ggcctcatcc
     1021 ggggcagcgc gatgtcgtcg caagccgcat ggctcgcttc cgtcttcttc ggattgttgg
     1081 ctgtcgttcg ccgacgtgga ggagcagttt cctgaacgcc gtggctggcc tggccaagtc
     1141 gaaaaacgcc gtttgcctcc ccatctccac aaaagtcaat caaatcgtgc ctttggctgc
     1201 tcgccgaggg aaccggggtc cttcgccgcg agcaagcgcg gcgaagcctg ggtatgtctt
     1261 gcggtcccct tcgcctcagg gatggccgtt ggcttcgtgc tccatgcgcg atcgcgatcg
     1321 gcttcgtcga ggttcgactc gagttgcgag aggttttcga attttcccgg cgatgaagaa
     1381 ccgctacttg ccgctctcga gcatcggaac gctcttggtg ttgtcggctt gtccaagggc
     1441 tttggctagg tcgagcatca gagccaggcc gcggagggtg tcggggtcgc gcatcttgcg
     1501 ccaaaggccg aacatggtga cctccgcacg accctggtca tcctgcgaaa tagcgtcggt
     1561 cgccgcgtgg gctaggcgca gggtgcgggg ctgggcaatc aggtccacga gttggagcaa
     1621 ctcgattagc ttgtcgccca aggccactac ccgctccggg gtgtagcgac ggctgaaccg
     1681 ctcagccacg gttctgatcg ccgccagatt cttgaagatt ccctgttttt ccagctctcc
     1741 tagctcggag atcgttgcct tcgtgacctc gtggatgatc ggggtcatgt cgctgataag
     1801 gtcgttggcc tgctcgagcc ggtcgagcag agcgctgaga ttcttcgtgt tgcggagaag
     1861 ccttttgatc aggagaaggg cgtcctcgag cttgaaatgg ccgtcgagtt cgtcgagttc
     1921 gaggatgacg gcatgcatca gctctttgag gatgggctgc gcgtcctcct tgagttcgag
     1981 cagcggcgcg aactgacgct cgagccgctc gagccgcgcc cacgtctcgt cgactttctg
     2041 gatcacctcg tcgtcacgtc caagggtcag tgccatggtt accccctaat cggcccactt
     2101 gccgaacatg gacatttgcg gctccagagg catttcctcg cctttgagca ggatgttcca
     2161 gtaaatccac ttgaacatca gcttgcccca atggttcatt cgcgtctcct ggagcagggc
     2221 aaaggggcct acgccaggca gcgggaaccg gcccggcaga ggctcgacgt cgtagttgaa
     2281 gtcgatgaga atgcctttct cgaagcctga ttcgataaag cagttggagt ggccgtcgaa
     2341 gaccgccctg ggctcgagcc cctcgatctc gcgcagcagg ttctcgcaga ggatctccga
     2401 tgcaaaatgg gccaccgagc ctgccttgga ggtgggcaag tcggtgttgt cgccgatgac
     2461 ataaacattc tccagatcct tcgccttgag cgtcgccttg tcggtaggca gataaccggc
     2521 gttgccgccg tcgaggttgc agtcctcgat cacctggggc ccaggttggg cgggatagcg
     2581 acgagaaggt cgaagttcac cttgcggccc tcggccccca gctcctcgat atatccttcc
     2641 gcggcgttca cctcgccgat attgaagttc ggggtgatga tgatgttttt gcgctcgcag
     2701 aagtcgccca ggatggccgc cgccttgggc ttggtgaagg cgtggggcag cggagtcacc
     2761 aattcgatct ccaccttgtc ccgaaccccc cgctcgtgga agaaccagtc ggccagaaaa
     2821 acgaactcca acggcgccac cgggcacttg aaagggatct ccgcgatgtt cagcacgagc
     2881 ctgcctttgt cgaagaattt caatgcctta gccagcgcta cggcaccgtc gagggagtaa
     2941 aaggtgtgga cgttcttgcc ccagccctcc atcatcccct ctacctcttc gggtgcgatg
     3001 cggcacccca gggccaccac caggaagtcg tagttaagct tacgcccttt cttggtcccg
     3061 acccgcttgt tcttcgggtc gatttcggtt acctcatcga gcacgaactc gacgccgggt
     3121 gggatgaagt cccgcttggg cttgagcacc atctccttgg tgtacatgcc aaaggggatg
     3181 aacagatagc ccggctgata gtggtgcatc tccggcgtcc accatggtga tggaccattc
     3241 ctccgcgcga agcgctttgc ggagtttggc cgccaccatg gtaccgccgg caccagcgcc
     3301 taagatcacg attttcttca tgtcttcttc ctctcccctc gcatcgactc caggagggat
     3361 ttaccattcg tgccatatca cgactgaatt ggcgggtaaa gcgatcatgt gttgcacagg
     3421 tgcaacgttc gtcgcgacct gccatggtat tcgcccttcg gcacggaggg atagtcggcg
     3481 ttgtccgggg cagttcgggc ctggaacaca aggcagcaca gttaccagcc gcggcgggat
     3541 gagcttttcc gcgccctgcc cactcaacgc ctcctcgtga gaggccatcg ttacttcttg
     3601 atccgcggga gcgctagatg atcgggataa atatcaaaga actccccttc tgttattgat
     3661 ctgttcaata tttttcagga acatcatatt cgatttcatt aagataaaat tttgcgtagc
     3721 tgtaatcacc gtctttaagt tggtacaacg cttcaatggc aaacgggatc atcattcccg
     3781 ctttttctct gtaatcggta acttttccca cccaaatctc caaatcattt tcgttcatgt
     3841 accgcttcgt tttgaattcg gtgatcgctc caaagtcatc aaaggaaacc aaataggaaa
     3901 tacaaatatt attataggtg aaattgagtc gagcgcttgt gccgtccatc ggcacccact
     3961 gcaggttatc tccgggtaaa aggttggtcg ggaaccatac gctttctgcc agccaccgaa
     4021 gtagttctcc ctgatcgtat atttccccct ggccgtcgac tatcccgaaa agtgaaagca
     4081 atgaaacggt cagcttgccc ctgttggaaa gatacatatc cctggctgtg aacattgttg
     4141 ttgaaccttt ccagacaaaa cccgggatct gtgtggtaaa atattgttct ccttttatat
     4201 ctacccactc cttcgagggg ccggttttga attgaccgct atgtttcaat ctagcgtagc
     4261 tgatgtaagg gcagccttct ttcaaggcaa accggaaa
//
LOCUS       DAOSOR010000508         4289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_20588_length_4289_cov_4.311290, whole genome shotgun sequence.
ACCESSION   DAOSOR010000508 DAOSOR010000000
VERSION     DAOSOR010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20588_length_4289_cov_4.311290"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PK710_20600"
     CDS             <1..315
                     /locus_tag="PK710_20600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12189.1"
                     /translation="PGDVATDDGLFADAPLRPIPQLAHDSLSTLNFGEGRVRNLLYGA
                     QPTQGAVAYVILSLLDNEDVEARVLRGAPPTDIEAVPVGWFGPELFGVFPLTRQSNSC
                     GF"
     gene            303..1376
                     /gene="tsaD"
                     /locus_tag="PK710_20605"
     CDS             303..1376
                     /gene="tsaD"
                     /locus_tag="PK710_20605"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOT12190.1"
                     /translation="MRILGIESSCDETAAAVVSHDGCIHSDVVRSQVSLHAPYGGVVP
                     ELASRDHIRGILPVVSEALSRAHLKLSEIDGIAVTCRPGLMGALLVGVQTAKGLAWGA
                     NKPFVGVDHLVGHLLASFLFEKDHAPDTQATFPYVALLASGGHTSLYRVDGPFPEQMR
                     ELGGTRDDAVGEAFDKVAKLLGLGYPGGPIIDQLAAQGNPEAVDVPRPMYGGKSLEMS
                     FSGIKTFVASHVATAGRPANERQLADLCASFQAAVIKTLVKKTLLAARAETIDTVVLG
                     GGVAANRGLRRSFQQACEKYGLKLVVPAPKRCTDNGAMIGYAGALELARGTRHDFDLG
                     VSTHTQLLRSTRRGGGKRPNRPR"
     gene            1373..1546
                     /locus_tag="PK710_20610"
     CDS             1373..1546
                     /locus_tag="PK710_20610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12191.1"
                     /translation="MSLRVCPIGATGEGANLLARFRAGGQGAGADEMGKCLSLCDFTG
                     HRKARPVPSVIVG"
     gene            complement(1590..>4289)
                     /locus_tag="PK710_20615"
     CDS             complement(1590..>4289)
                     /locus_tag="PK710_20615"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOT12192.1"
                     /translation="YALWPRRAFLQVAIPSIVASFGLVLIISGYSYWQGGVCFGPRHL
                     VPSIPLIALGLAHIPQTWLSKPWFWVVASLSFGVNLLGVATTPFIAERQTAPLSLYLK
                     LAKEGALALNPSGFMAPSSERRWRWAAMAKDGTGAFNLGETLGLQGWWSLLPWLLVVV
                     ILMVVFVYIVKPRGDQDEHRNRGSLSVAFILEFFSLQMLKNTIKSSISFRTKRGQGGV
                     LRIASAALFFFSLLLLRFQVVQDLGTGVRQAFHQGLITGIFADFFLTLLVLAGAWCAA
                     WMVVPELFWLAFIALGVVVWSAEIAGVLYYSFFGEPLEWWIVVRQSAEIPLIVDSALA
                     LGRTPFIVGSALLLLSSLGLSGFFLLRAWRIWRKSASPPTEESPIPAVPAKTSLFPGL
                     LLGMISVLFMNSLASSSAARGLLDESTVTRWWRQASGHDKEASRWPNVRNPAQQLIIF
                     RQLESGTEPQNLLPAAWLQPTVEASSSEGNPWPLARRLSFEPKDIAEMREPLGIPTGA
                     MVNMIVIFVESWRSFEFEHPRLGPLVFPRLRARFAQQATYFPFTYASIYPEASTARGQ
                     YSALCSSLPNLGGPPVFLRFTGINVQCLPQLMGNGGYHTVLMSAGANEFHKTKAFFST
                     HGVQTFVGREYFRQFDDPDRVGTWPQQVLSDRPFLHEALQLLEHPNQEQPIRKPFLAV
                     LATLSSHHPYVGVLPDVDLPGADRDYLGYLATLHYVDEALDLFLSELFSTQLADDTLV
                     ALIGDHGIRLPPVESLTKLQQLELISRVPMALITAKPQSSRRIPAPVHQMDLAPILTA
                     IAGVPGKVSWIGRNALLGSGSPWVSSRHDSFAYRLGPHECFGNPNERPRCYHLKPGQD
                     PLFDQNLEETDEVPTLTSFMHRVVKANQRLIQYDLLAPPDAEP"
BASE COUNT          996 a         1177 c         1269 g          847 t
ORIGIN      
        1 ccgggagatg tcgctaccga tgatggcctc tttgccgatg cgccgttgcg tcccattcca
       61 caactcgccc acgattccct ttccacattg aatttcggcg aggggcgcgt gcgcaatctg
      121 ctgtatggcg cccaacccac gcaaggggct gtcgcgtatg tcatcctttc gctgctcgac
      181 aacgaagatg tggaagctcg tgtgttgcgt ggtgcgccgc ctaccgacat cgaagccgtg
      241 cccgttggtt ggttcggccc tgaattattc ggcgttttcc ctctcacaag gcaatcgaat
      301 tcgtgcggat tttaggtatt gaatcctcct gcgacgaaac cgccgccgcg gtagtttccc
      361 atgacggttg catccacagt gacgtggttc gtagccaggt gtcgttgcac gctccgtacg
      421 gcggcgtggt gccagaactg gcatcccgcg accatattcg cggtatcctc ccggtcgtaa
      481 gcgaagccct gtctcgcgcc catctgaaac tcagcgaaat cgatggtatc gcggtcacct
      541 gccgtcctgg attgatgggc gcgttgctcg tgggagtaca aaccgccaaa gggctcgcct
      601 ggggtgccaa caagcctttc gtgggcgtcg atcacctcgt gggtcacttg cttgcttcgt
      661 ttctttttga aaaagaccat gcgcccgaca cccaggccac gttcccctat gtggctttgc
      721 ttgcttccgg cgggcatacc tccttgtacc gagtggatgg ccctttcccg gagcagatgc
      781 gcgaactggg aggtacccgc gacgatgcgg tgggagaggc tttcgacaag gtcgccaagc
      841 tgcttgggct aggctacccg ggaggcccca tcatcgacca gctcgcggcc cagggcaatc
      901 cggaggcggt cgacgttccg cgacccatgt atgggggaaa atcgctcgaa atgagctttt
      961 ccggcatcaa aacgtttgtg gcgtctcatg tagccacggc cggacggccc gccaacgaac
     1021 gccaattggc tgacttatgt gcctcgtttc aagctgccgt gatcaaaacc ctggtcaaaa
     1081 aaacgctgct cgccgcgcgg gccgaaacga tcgacaccgt cgtgctcggc ggtggcgtgg
     1141 ccgcgaatcg tgggcttcga cgttccttcc aacaagcttg tgagaaatac gggctgaaat
     1201 tggtggtgcc cgcacccaag cgctgtacgg ataatggtgc catgatcggt tacgccgggg
     1261 cgctggaact ggcccgcgga acccgacatg acttcgatct cggagtgtcg acccataccc
     1321 aattgcttcg ttccacccgg cgggggggcg gaaaaagacc caatcgtcca cgatgagcct
     1381 gcgagtctgc cccatcggag cgacagggga aggggcgaac ctgttggcgc gttttcgggc
     1441 cggagggcaa ggagcaggag cggatgagat gggtaagtgt ttgagtttat gtgattttac
     1501 agggcatcga aaggcgaggc ccgtgccatc ggtgatagta ggatagtgaa cgcttataca
     1561 agggtggttg gacgtctggc cgcgcagggt taaggctctg catcgggtgg agccaataaa
     1621 tcatactgaa tcaaccgttg gttagccttg accacccgat gcataaacga cgtcaacgta
     1681 gggacttcat cggtttcttc gaggttctgg tcgaaaaggg ggtcttggcc cggcttcagg
     1741 tgataacaac gcggtcgttc attgggattt ccaaagcatt cgtggggtcc aaggcggtag
     1801 gcgaaagagt catgacgaga actcacccaa ggagatccgc tgccgagtaa ggcgttgcga
     1861 ccgatccagg atacctttcc aggcacgcca gcaatcgcgg taaggatcgg ggctagatcc
     1921 atttggtgta ccggagcggg aattcgtcgt gaactctggg gtttggccgt gatgagcgcc
     1981 atgggtaccc gtgagataag ctcgagttgc tgcaatttcg tgagtgattc gacgggaggg
     2041 agccggatcc cgtggtctcc gataagcgcc accagtgtgt cgtcggcgag ctgagtcgaa
     2101 aaaagctccg acaaaaaaag atcgagagcc tcatcgacat aatgcagagt agcgagatag
     2161 ccgagataat cgcggtctgc cccgggcagg tctacgtccg gcaacactcc cacataagga
     2221 tgatgagaac tgagcgtagc caacacggcc aaaaaaggtt tgcggatggg ctgttcttga
     2281 ttggggtgtt cgagaagttg caaagcttcg tgcaaaaaag ggcgatccga caggacctgt
     2341 tggggccacg tgcctacgcg atccggatca tcgaattgtc ggaaatactc acggccgaca
     2401 aaagtctgca ccccgtgtgt cgagaaaaat gccttggttt tgtgaaattc gttggcgccg
     2461 gcgctcatca aaaccgtgtg gtatccgccg tttcccatca gttggggcag acactgcacg
     2521 ttgattccgg tgaaacgcag aaataccggt gggccaccga gattgggaag actcgagcat
     2581 agggcactgt actgaccgcg agccgtactt gcctcgggat agatactcgc atacgtgaaa
     2641 ggaaaatacg tggcttgttg cgcgaaacgt gcgcgtagac gcggaaaaac cagcggtccc
     2701 agtcgcgggt gctcgaactc gaagcttcgc caggactcca cgaatattac gatcatattg
     2761 accatggcac ccgtggggat gcccaggggc tcacgcattt cagcaatatc tttgggttcg
     2821 aacgatagcc ttcgagcaag cggccatgga ttcccctccg aagagcttgc ctctaccgtg
     2881 ggctgaagcc acgccgcggg aagcaagttt tgtggctcgg tccctgactc cagttgccgg
     2941 aagatgatta gttgttgggc gggatttcgc acgttgggcc aacgggatgc ctccttgtca
     3001 tggcccgacg cctgacgcca ccatcgggtc accgttgact cgtcgagcaa acccctggct
     3061 gccgaagagg aggccagcga gttcatgaac aacacactga tcatccccaa gaggagacca
     3121 ggaaataagg aggttttcgc cgggaccgcg gggatagggc tctcttccgt cggaggagaa
     3181 gcgctttttc tccatatccg ccacgcccgc aggaggaaga atccagacaa accgagcgaa
     3241 gacaacagca acagggcgct gcccacgatg aatggtgtgc gtcccaacgc tagtgccgag
     3301 tcgacgatca aggggatctc agcgctttgg cgcacaacaa tccaccattc aaggggctcg
     3361 ccgaagaagc tgtaatacaa cacaccggca atttccgcgc tccataccac cacccccaat
     3421 gcgataaaag cgagccaaaa taactcggga accaccatcc acgctgcgca ccaagctcct
     3481 gccaaaacca gaagcgtaag gaaaaaatcg gcgaaaatac cggtaatcaa cccctgatgg
     3541 aaggcttgcc gcacccctgt tcccaaatcc tgcaccacct gaaaccgtaa cagcagcaag
     3601 ctgaaaaaaa acagcgccgc cgacgcgatg cggagcacac cgccctggcc cctcttggtt
     3661 cgaaaggaaa ttgatgattt tatggtattt ttgagcattt gtagggaaaa aaattctaga
     3721 atgaaagcaa cactgagtga cccccggttc cgatgctcgt cctgatctcc acgtggtttt
     3781 acgatgtaaa caaagaccac catgagaatg acgacaacca acagccaggg cagcagggac
     3841 caccagcctt gcaatcctag agtttcccct agattgaaag ccccagtgcc gtccttggcc
     3901 atggcggccc agcgccagcg tcgctccgac gatggggcca tgaacccgga gggattgagg
     3961 gccagagcac cttctttggc gagtttgaga taaagggaga gaggagcagt ttgccgttcc
     4021 gcgatgaaag gggtggtcgc cacgccaagc aggttgacac cgaaggatag cgaggcaact
     4081 acccagaacc aaggcttgga tagccatgtt tgcggtatat gagccaggcc aagagcgatg
     4141 agcggtatcg aagggacaag gtgtcgaggc ccgaaacaaa cgccgccttg ccaataggaa
     4201 tagccagaaa taatgagtac gagtccgaaa gatgccacga tcgaagggat tgctacctgc
     4261 aagaaggctc gacgaggcca cagggcata
//
LOCUS       DAOSOR010000509         4287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_20601_length_4287_cov_4.261815, whole genome shotgun sequence.
ACCESSION   DAOSOR010000509 DAOSOR010000000
VERSION     DAOSOR010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20601_length_4287_cov_4.261815"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            278..1390
                     /locus_tag="PK710_20620"
     CDS             278..1390
                     /locus_tag="PK710_20620"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOT12193.1"
                     /translation="MPKVSMSSLNQSIDAAGMTDAGTVREHNEDAFLVRNDLCLFMVA
                     DGAGGHNAGDIASKLAIKSIENFFEATESVAKTRPERDRFGLMTQARRLAAAVQKANA
                     DVMEISRSFEKRKGMGTTVVVAYLRPSWNRLYLAHVGDSRCYRVRGEHVEVLTHDHTI
                     LNDVMEMRPDVGDDVLARLPRSVVTRALGMGERIRVDLRAFVLVPGDRYLLCSDGLYR
                     DLPEDVLFETLRITAEPEEVAQRLIALGKKAGGGDNLTALVFDCVRLGKPSHDKQGAD
                     SSSTRRKTASAPEILLLGIESDVDIQPTGEVQILPSDAYDAEVRQAVEDFVSPLRPDH
                     AALPIDLTKTSQCGACGEQIPVGARFCPYCGAPRKT"
     gene            1471..1995
                     /locus_tag="PK710_20625"
     CDS             1471..1995
                     /locus_tag="PK710_20625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12194.1"
                     /translation="MLRTVRMSVWLTWIGVVGVSAASCAQAQDPDAVVDKPEAGVGGS
                     AGQATGGTGGQLEDSSSEADGGEAGMGAMGGSNPGGTGGAGGNGGTGGTGGYGGTGGV
                     PVDAEPEAGIVCGTEICTDWLCEKANIGVSMTVKACCQKTNECGAVNEEGGSLCVPVE
                     VVETLLKMTCSPIE"
     gene            complement(2012..3760)
                     /gene="rpoD"
                     /locus_tag="PK710_20630"
     CDS             complement(2012..3760)
                     /gene="rpoD"
                     /locus_tag="PK710_20630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOT12195.1"
                     /translation="MPSVQTNRCPGSCKKSIPSHPFAQLVALGKQQGFLTYDDVHASL
                     PDSMMSADRVSDLMTELEREHIEVVPSQVHDSTHRSSLDPHRDTEKRLVDTSGMRDPA
                     DMYLRKLGGAPLLTREGEVELAKRIEDGERRMVAAMLQCHSTMREIIRLGERVEAGKT
                     RIADVLRDCDDEDDAEKGRFVRVAREIAVLHHERLQLLESARKANAKGSSGKRHLSGA
                     ERKLKAAIALLHDVRLDRKQLDRFVAKLYSLQRRIDDAEKQLERCECEAGIPFKTIVK
                     LIDQQGNGTSRSTTGGPKRLALDELQEYADTIRTLLTQAQTAEEEAGIDRVQLRSVLR
                     EVRRSQRVSERAKAELVEANLRLVVSIAKKYPGRGLDFLDRIQEGNIGLMKAVDKFDH
                     RRGYKFSTYATWWIRQAINRAIADQARTIRLPVHMFEAINKIARITRLHVQETGIEPS
                     PEEIAEKMDLPVDKIRKYMRTVQDPVSLETPVGDDEDRHLVDFVEDKRTVGPEDGVIN
                     IDFNSTTRQMLQTLSPREEKVLRLRFGIDEKSDNTLEEVGQSFNVTRERIRQIEAKAL
                     HKLRHPKRAEQLRHFL"
BASE COUNT          922 a         1124 c         1279 g          962 t
ORIGIN      
        1 tctccaaaag gatggaagcg gaaggagtca aacccatgct cgatgtcgtg ccaggagacc
       61 attcctacga cttcaatcgc ggccctggcg tgtacgagat gcttctgttt cacgaacgcg
      121 tgcttcgagg cgaacatgct cccggcacgc agggccgtta atcctcatgt cgcaggagcc
      181 aacaccacga acccctccga tttccatgac ggatgggagt ttcacgtgcg ttgcactttt
      241 gcgttggacc taatccggtt actcatatac tcacgcgatg cccaaggttt cgatgtcctc
      301 tctcaatcaa tcaatcgatg ccgctggaat gacagacgcc ggaacggtgc gagagcataa
      361 cgaagatgct tttctcgttc gcaatgactt gtgtttattc atggttgccg atggggcagg
      421 ggggcataac gcaggcgata tcgcgagcaa gctggcgatc aaatcgatcg agaacttctt
      481 cgaagccacg gaaagcgtag ccaaaactcg tccggaacga gaccgtttcg ggctgatgac
      541 acaagcaaga agactggccg cggccgtaca gaaggctaac gcggacgtca tggaaatctc
      601 ccgctcgttc gaaaagcgaa aggggatggg aacgacggtt gtggtagcgt atctgcgccc
      661 atcttggaat cgcctgtatc ttgcgcatgt gggagatagc cgctgttatc gcgtgcgagg
      721 tgagcacgtg gaggtgctga cccatgacca cacgatactc aacgatgtca tggagatgcg
      781 tccggacgtg ggtgatgacg ttttggcccg gcttccccgc agtgtcgtaa cccgggccct
      841 gggaatgggc gaacggattc gtgtggattt acgagccttt gtcttggttc cgggagatcg
      901 atatctactt tgcagtgatg ggctgtaccg ggatttgccg gaagatgtgc tgttcgaaac
      961 gcttcgcata acggcagaac cggaggaggt ggcgcaacgg ctcattgctt tgggaaaaaa
     1021 agcgggggga ggcgacaatc tcacggctct ggtcttcgat tgcgtccgac tcggcaagcc
     1081 aagccacgac aagcagggcg ccgattcatc ttcgactcgg agaaaaacgg cttccgcgcc
     1141 agaaatactg ctgctcggaa tcgaaagcga tgtcgatatt cagccaacgg gtgaagtgca
     1201 gattctgccg agcgatgcat acgatgccga ggtgcgacaa gcggtggaag atttcgtatc
     1261 tcccttgagg cccgatcacg cggcccttcc catcgacctg acgaaaacat cgcagtgcgg
     1321 tgcttgcggt gaacaaatcc ccgtgggagc gcgattctgt ccgtattgtg gagcgccgcg
     1381 aaagacgtga cgggatcggt tcgaggcacg ataccctgag actcgattct gaaccttgct
     1441 ccaagccgtt ctggcatcta cgatgattgt atgctgcgta ccgtgcggat gagtgtatgg
     1501 cttacttgga ttggtgttgt gggcgtcagt gctgcttctt gtgctcaggc gcaagacccg
     1561 gatgctgtcg ttgataagcc tgaggcaggg gtgggcggct ctgccggtca agccacggga
     1621 ggcaccggag gccaactcga ggatagttcc tccgaagcag atggcggtga agcaggaatg
     1681 ggagccatgg ggggctccaa ccccggtgga acgggtggag caggcgggaa cggtggcaca
     1741 ggcggcacag gcggctacgg cggaactggc ggcgttcctg tcgatgccga accggaagca
     1801 ggtattgtct gcggcacgga aatctgtacc gattggctct gtgaaaaggc caatatcggc
     1861 gtgtcgatga ccgtgaaggc ttgctgtcag aaaaccaacg aatgcggcgc cgtcaacgaa
     1921 gaaggaggca gtctctgtgt ccccgtcgag gtggtggaga ctctgctcaa gatgacctgt
     1981 tcacccatcg agtgaaaggc tatccggaaa atcacaggaa gtgacgaagc tgctcagcgc
     2041 gcttgggatg gcgaagcttg tgcaacgctt tcgcttctat ttgacgaatg cgttcacgcg
     2101 ttacgttgaa gctctgccct acctcctcca acgtgttatc cgatttttcg tcgataccga
     2161 aacgaagtcg caacaccttc tcttcacgag gagagagcgt ttgcagcatc tgccgcgtgg
     2221 tggagttgaa gtcgatgttg atgacgccat cttcgggacc aaccgttcgt ttgtcctcga
     2281 cgaagtcgac gagatgacgg tcttcatcat ctcccaccgg tgtttccaaa gacactgggt
     2341 cttggaccgt tcgcatgtat ttgcggattt tgtcgaccgg cagatccatc ttttcggcga
     2401 tttcttcggg cgacggttcg atgccggtct cctggacatg aagccttgtg atacgcgcga
     2461 ttttgttgat ggcctcgaac atgtggacgg gaagacggat ggttcgcgcc tggtccgcaa
     2521 tggctcgatt gatggcttgt cggatccacc aggtggcgta ggtggagaac ttataacctc
     2581 gacggtggtc gaatttgtcc acggccttca tcaaaccgat gttgccttcc tgaattcggt
     2641 cgagaaagtc gagacctcgg cccggatact tcttggcaat ggaaaccacg agacgcaagt
     2701 tggcttcgac gagttcggct ttggcgcgtt cggagactcg ttgactgcgg cgtacttctc
     2761 gcagaacact gcggagctga acgcgatcga tcccggcttc ttcttcggcg gtttgcgctt
     2821 gggtcaggag cgttcgaatg gtgtcggcgt attcctggag ttcgtcgaga gcgagacgtt
     2881 tggggccgcc cgtagtcgaa cgagatgtcc cgtttccttg ttgatcgatg agcttgacga
     2941 tggttttgaa agggatcccc gcttcgcact cgcaacgttc gagctgtttt tccgcgtcgt
     3001 cgattcgacg ctgaagcgag tatagcttgg cgacgaatcg atccagttgt ttgcgatcga
     3061 ggcgaacgtc atgaagcaat gcaatggcgg ccttgagttt tcgttcggca ccagacaagt
     3121 gacgcttgcc cgaggagcct tttgcgtttg ctttgcgcgc agattcgagc aattgcaggc
     3181 gctcgtggtg aagaaccgcg atttctctgg caacgcggac gaagcgaccc ttctccgcat
     3241 catcttcgtc gtcacagtcc cgaagcacat ccgctatccg ggtcttgccg gcttcgacac
     3301 gctcccccaa gcgaatgatt tcacgcatcg tactatgaca ttggagcatg gcggcaacca
     3361 tacgccgctc tccgtcttcg atacgcttgg cgagttcgac ttctccttcg cgggtcagaa
     3421 gcggagcacc accgagtttg cgtagataca tatccgcggg gtcacgcatg ccacttgtgt
     3481 ccaccagtcg cttctcagtg tctcgatggg gatcgaggga agaacggtgg gttgagtcat
     3541 gaacctggga aggaacgact tcgatatgct cgcgttcgag ttctgtcatc aaatcactca
     3601 cacggtcggc tgacatcata gaatccggaa gtgaagcgtg aacgtcatca taggtgagga
     3661 atccttgctg ttttccgaga gcaacgagtt gggcaaatgg gtgcgacgga atcgacttct
     3721 tgcaggaacc cggacaccta ttggtttgga ctgaaggcat cagagaacct ccgacactgg
     3781 ggtaaaaagg aaccacaatc gttttggcga gccgcgaggg ctgactcgca tccggctctc
     3841 accgcaggat gcgccgcttc gacgcatcag ccattgagag ccgcggggct ttagcgcggc
     3901 aagaaggtgg atgccagaaa atcgacatcg agcgatgctt tttctttcga caagcccccc
     3961 agcgttgcgc taactatccg aaagttgtgt cgaaagacgc acggggacgc gtctttcaag
     4021 gctgcaaaat cgcaagccaa cgggcacaaa tcctccttct gccgtcactc tgcgacggaa
     4081 tattttcctt taatttcgtt ttattgggaa ctcgatcgct ccccttcgag ccgcgcaatc
     4141 cccctatcgc aacgggggag agtggggcaa agaagcgacg aaaattgggc agtaaaccac
     4201 gagctgcacc cgcgcgcgtc acacatcagt tgagttcagt caaattcaac ccgcccggat
     4261 accagtagct cgtgtactgt ccatata
//
LOCUS       DAOSOR010000510         4271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_20712_length_4271_cov_4.115987, whole genome shotgun sequence.
ACCESSION   DAOSOR010000510 DAOSOR010000000
VERSION     DAOSOR010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20712_length_4271_cov_4.115987"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PK710_20635"
     CDS             <1..461
                     /locus_tag="PK710_20635"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOT12196.1"
                     /translation="DGSRMSILIQSFGFKYGPPIDADLMLDVRFLPNPYFVEGLRQQT
                     GLDEPVETYVMGFPDTKAFLDRAQSLISFCIPRYEREGKAYLTIAVGCTGGRHRSVAT
                     AVELARRLEETLGTAIGVAHRDIHRDVSSDRASEPDIIGGGVRGMGRAVR"
     gene            458..745
                     /locus_tag="PK710_20640"
     CDS             458..745
                     /locus_tag="PK710_20640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOT12197.1"
                     /translation="MSIRMQEAQAKFEIINELGLHARAATKLVQLAARFPCEIEITRD
                     GQRANAKSVMGVLLLCGVKGVVLDVVARGERAHDAVEAISVLIADRFGESQ"
     gene            742..2544
                     /gene="ptsP"
                     /locus_tag="PK710_20645"
     CDS             742..2544
                     /gene="ptsP"
                     /locus_tag="PK710_20645"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOT12198.1"
                     /translation="MNSERPSAYPGASVTLRGLCGSPGISMGPAIVWSRAAPRRHWRR
                     VDQHEVEFEIQRFHDAVRDGSERIRTVIAAASPRARAETAILDAYLLMLGDLALRQDV
                     EQKIRFERKSCEWAVSATIQSYAKQLEETADPYLRERRHDVEFIGDLLLRALGGGREE
                     RPGIEIDRPSILVAHDLSPADMAAFNHEFVLGIATERGTRIGHTAIIARALEIPAIVG
                     TVELLNNVNQGDEVIIDGFRARVIVRPTKEQSERAVQQAQWHRKRKDRLKQSVALPVA
                     MACGTPITVRANVELAAEVPLAIHRGAQGVGLVRTEFLFIDRSSLPSEQEQYETYREI
                     VQSAAPQMVTLRTFDIGGDKFVSSLCMPPELNPALGLRAIRLGLFHPEILETQLTAMV
                     RASAHGPVRILLPMVSCLSELRDVKQLLRSVIETVDRDGHARAEEIPLGVMVEVPSAV
                     VLADRFAEESDFLSLGTNDLIQYVFAVDRTNRSLAYLASAFDPSILRLVRAASQAALR
                     HDKPLSVCGEMASDPLGAILLVGLGIRELSMEGGAVAEIKECLRRVFHQEAEQVALDC
                     MNLDTAEQVEQHVARSFAPRLADLLQGDYISDKA"
     gene            complement(2555..3346)
                     /locus_tag="PK710_20650"
     CDS             complement(2555..3346)
                     /locus_tag="PK710_20650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12199.1"
                     /translation="MTGRFVRACVWTFAAVGFVACGPKPPPENAGRGTVESEFNRCVD
                     QEKYGEAKGCWQAFLDRYGDVASSAERAYAKEHLEQSATRATSPAAQEEDAVTRGAKA
                     TEGLVRLQEKDGASSSGSGFPTQRVAFDECYKGFSVTGHSERDVIALGERCGAPCGMI
                     PFSSVRSDSQRESDDVDVYGIELRANRCYRFIAVGDSGIDDLDAAIADSEGNILLRDT
                     YSDSAPILGPESAFCPSSAGHYKFVVSVARGAGTFHFQVWQGRRQ"
     gene            complement(3376..>4271)
                     /locus_tag="PK710_20655"
     CDS             complement(3376..>4271)
                     /locus_tag="PK710_20655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12200.1"
                     /translation="LQTKLPLSVASFSTYLSQGKRLRVRFATAGSFELVNDFSISDEG
                     ACGEATHFVQTISVGAYRVSELTDKGGGAEVSTTGPSVGGRHSDMSQHNVGMGDLDAC
                     ATATTDAPPKNCQMPLEILMVPIGSQRVPKPAPSSRGDDPAPSPSVMPTPVSTDRSAG
                     GMTHENCYVGIDLTRDAPTDLAAIVQRCGAPMGMSPVGGAIIDSLSAGQPMHEYGVDV
                     EAGSCYRIFAVGGRGIEDLDTGLKDPAGEWVSKDILDDAFPILNPNGPFCVRQGGRHK
                     LLVSVAKGSGTYAVQLWKVNR"
BASE COUNT          897 a         1124 c         1288 g          962 t
ORIGIN      
        1 aggatggcag ccgcatgtcg atcctcatcc agtcgttcgg tttcaagtac gggccgccga
       61 ttgatgcgga tttgatgctg gatgtccgat tcttacccaa tccatatttt gtcgaaggtc
      121 ttcggcaaca gacaggattg gatgagccgg tagagacgta tgtcatgggt tttccagata
      181 ccaaggcatt tttggaccga gcgcagagcc tcatttcgtt ttgtattccg aggtatgaac
      241 gcgaggggaa agcctatttg acgattgcgg taggttgtac gggcggtcga catcggtcgg
      301 tggcgacggc ggtcgaattg gcacggagac tggaagaaac gttgggaacg gcaattggcg
      361 tggctcatcg tgatattcat cgcgacgtgt cttccgaccg ggcatcggaa ccggatatca
      421 taggaggtgg agttcgcgga atgggccgcg cggttcgatg agcatacgaa tgcaagaagc
      481 gcaagccaag ttcgagatca tcaatgagtt ggggcttcac gctcgtgcgg ccacgaagct
      541 cgttcaactt gccgcgcgat tcccttgtga gattgagatt acgcgcgacg gtcagcgcgc
      601 caatgcaaag agcgtcatgg gggtattgct cttatgcggc gtcaaaggcg tggtgcttga
      661 tgtggtggcg cggggcgaac gtgctcacga tgcggtggaa gcgattagtg tattgattgc
      721 tgaccgattt ggggaatccc aatgaacagc gagcgaccga gcgcgtatcc gggcgcttcg
      781 gtcacacttc gcgggctttg cggttctcct gggatctcca tgggtcccgc gattgtatgg
      841 agtcgagcgg cacctcgtcg ccattggcga cgggttgatc agcacgaggt ggagttcgag
      901 attcagcgat tccacgatgc tgtgcgtgat ggatcggaac ggattcgtac cgtcattgcc
      961 gcggccagtc cgcgagcgag ggcagagaca gccattcttg acgcgtattt gctcatgctt
     1021 ggcgatctcg cgttgcggca agatgtggaa caaaaaatcc gattcgagcg aaagagctgc
     1081 gaatgggcgg tttcggcaac gatacagagc tatgccaagc agctagagga aaccgcggat
     1141 ccgtacctac gcgagcgtcg acacgacgtt gagttcattg gagacctttt gcttcgcgcg
     1201 ctgggcggtg gacgcgaaga gcgtcccggt atcgagattg accggccgag tattctggtg
     1261 gctcacgatt tgtcgccagc cgacatggcg gcttttaacc atgagttcgt tcttggcatt
     1321 gccaccgaaa ggggcacgcg aatcggtcac accgcgatca tcgcccgtgc tctcgaaatt
     1381 ccggcgattg taggaaccgt cgagttgctg aacaacgtca accagggaga tgaggtcatc
     1441 atcgacgggt tccgtgctcg tgtcattgtt cgaccgacca aagaacaatc cgaacgcgcg
     1501 gttcagcaag cccaatggca tcgcaagcga aaggatcgac tcaaacaatc ggtggcgctg
     1561 ccggtagcga tggcgtgcgg cactcccatc acggttcgcg ccaatgtcga gcttgccgcg
     1621 gaagtccccc ttgccatcca tcgaggcgcc cagggagtgg gattggtccg taccgagttt
     1681 cttttcattg atcgatcgtc gttaccctcg gaacaagagc aatacgagac gtatcgggag
     1741 attgtgcagt ccgcggctcc acagatggtg actttgcgaa ccttcgatat cggaggtgac
     1801 aaattcgtat cgtcgttgtg tatgcctcct gaactcaatc ctgcgcttgg tttgcgagcc
     1861 attcgacttg gcttatttca tccggagatc cttgaaacgc agcttacggc catggttcgt
     1921 gccagcgctc acggacccgt acgaattctg ttgcccatgg tttcttgcct ttcggagctt
     1981 cgcgacgtca agcagttgct tcggagtgtc atcgaaacgg tggatcgtga tgggcatgct
     2041 cgagccgaag aaatcccgtt gggtgtcatg gtggaagtac cgtcggcggt ggttcttgct
     2101 gaccgttttg ccgaggaatc ggattttttg agtttgggga ccaacgatct cattcaatat
     2161 gtattcgcgg tggatcggac gaatcgttct ctggcatatt tggcaagcgc atttgatcca
     2221 tcgatattgc gtttggttcg agccgcttcc caagctgctt tacggcacga caagccattg
     2281 tcggtttgcg gcgagatggc gtccgatccc cttggagcga tcctgttggt tggtttggga
     2341 atacgcgagc tatcgatgga gggcggtgcg gtggcggaaa tcaaagaatg ccttcgtcgt
     2401 gtatttcatc aagaagccga gcaagtcgcg ttggattgca tgaacctgga tacggcggag
     2461 caagtggaac agcacgtagc gcgttccttt gcgccacgtc tcgcggattt gttgcaaggc
     2521 gattacataa gcgacaaggc atgaagcgcg tgaatcattg acgacggccc tgccagactt
     2581 ggaagtggaa cgtccctgcc ccgcgcgcca ccgaaactac gaatttataa tgaccggcgg
     2641 agcttgggca gaatgcgctt tcgggaccaa ggatcggggc agagtccgaa tatgtatcac
     2701 gcaacaggat atttccttcg gagtccgcaa tggcggcatc caagtcatca atgccgctat
     2761 cgccgacagc aatgaaccgg taacagcggt tcgcgcgcaa ctcgataccg tacacgtcga
     2821 catcgtcgct ttcccgttgg ctatctgatc tgacactcga aaacgggatc attccgcagg
     2881 gagcgccaca acgttcaccg agtgcgatga catcgcgttc cgaatggccg gtgacgctaa
     2941 agcctttgta gcattcgtcg aaggcaacgc gctgcgtggg aaagccggaa ccactggagg
     3001 aggcgccatc tttttcctgc aatcgcacga gaccttccgt tgcttttgca ccgcgtgtga
     3061 cggcatcttc ttcttgtgca gcgggggagg tggcgcgcgt tgcggattgc tccaggtgtt
     3121 ctttggcgta ggctctctcc gccgaggagg cgacatcacc gtagcggtcc aagaaagcct
     3181 gccaacaccc tttggcttcc ccgtactttt cctggtcgac acatcggttg aattcggatt
     3241 ctaccgtgcc gcgcccggcg ttttccggcg gcggtttggg tccgcaagca acgaagccca
     3301 ccgcggcaaa cgtccaaaca caagcccgaa cgaaacgacc ggtcatggtt gtttcccctt
     3361 cctaccttac tttttctaac ggttgacttt ccaaagctga acagcgtaag tccccgatcc
     3421 tttggcgacc gagacaagca gtttatgccg tcctccctgt cgtacacaga agggaccatt
     3481 gggattgaga atggggaagg catcatcgag gatatccttg gaaacccatt caccagcggg
     3541 gtctttgaga cccgtgtcga gatcttcgat cccacgtcca ccgaccgcga agatgcgata
     3601 acacgagcca gcttccacat cgacgccgta ctcatgcatg ggctgaccag cggatagcga
     3661 atcgatgatg gcgccaccca cgggggacat acccatgggg gctccgcagc gctggacaat
     3721 ggcagcgaga tcggtggggg catcccgtgt caaatcgata cccacgtagc agttttcgtg
     3781 agtcatcccg cccgcggaac ggtcggtgga tacgggtgtt ggcatgacac tgggagaagg
     3841 ggctgggtca tccccgcgcg atgagggagc cggttttggg acgcgttgag agccaatcgg
     3901 aaccatcaaa atttcaagag gcatctgaca gttcttaggg ggcgcatcgg tcgtggcagt
     3961 cgcgcaggcg tccagatcac ccatgccgac attgtgttga ctcatatcgc tgtgacgccc
     4021 tccgacggaa ggcccggtcg tgcttacttc cgcaccaccg cctttgtcgg tgagttcgga
     4081 gactctgtaa gcgcccacac tgatcgtctg aacgaaatgg gtggcttccc cacaagctcc
     4141 ttcatcggag atcgagaaat cgttgacgag ttcgaacgaa cctgctgttg caaaacgcac
     4201 gcgcaatcgt tttccttgag aaagatacgt ggagaagctc gcgacgctca gaggcagctt
     4261 cgtttgaagg t
//
LOCUS       DAOSOR010000511         4262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_20779_length_4262_cov_3.500119, whole genome shotgun sequence.
ACCESSION   DAOSOR010000511 DAOSOR010000000
VERSION     DAOSOR010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20779_length_4262_cov_3.500119"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PK710_20660"
     CDS             <1..315
                     /locus_tag="PK710_20660"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HOT12201.1"
                     /translation="ADEPDHKVKRLVVYPGKRLSLQRHRHRSEHWIVLEGEAMVVQDD
                     EEVRMLLGQSIEIPKGCWHRIRNPGMENLAIIEVQTGEYFGEEDIERKEDDFGRAPPL
                     SL"
     gene            465..1847
                     /locus_tag="PK710_20665"
     CDS             465..1847
                     /locus_tag="PK710_20665"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOT12202.1"
                     /translation="MYRPIIRFVSTAAVMVMMGCDQRGPAAPSSEPTGNTTMPAPTEQ
                     ATQETITEHAKPAPPIAEDSKQAATSTPAGRRPLNVILLVIDAMRDDMPWNGYDRPIA
                     PNLTALHAKSVSYERGYAISSFTSKSVAGLLSGQYPSSLDRTTPFFTSYRDKTNTFMA
                     EVLRDNGVRTLGIQAHLYLKEKSGLHQGFDDWRIVPGIEWDPNKDPFITSPQHTASVM
                     EQLSDPKNVQGKFFAYYHYMDPHDIYNSHEQAPKWGTTPRDRYDEEIWFADYHIQKAL
                     EFVGSQPWGKDTAIIVTADHGEAFGEHGFYRHARHLYEVLIRVPLFVHIPGVEPRKIA
                     RWRSHIDIVPTVYDMMGVPIPEGLPGTSLLPEILGEEVPQRPIVADLPADNINSRRRA
                     LIDDGYKLVAHGQDVRFEMYDVRNDPAETTNLFRKDKARAHAIREQYRKVSEAIPFKK
                     ARGGPAVKKD"
     gene            2008..2949
                     /locus_tag="PK710_20670"
     CDS             2008..2949
                     /locus_tag="PK710_20670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12203.1"
                     /translation="MNQRWAVAGLMMACVTIGCGGSSVPSAKSSSGYEEYTCPEPIGK
                     IVREDCSGVGLRYDGERFEGSVGVGGVGASASYQQTAIRQADSLIQLLKEQRVAMCNN
                     FNTCKLTVAEYREDQKRIDDSFIALMSLKDRMKDMDADQASQLLREIQAIRTGVQNVA
                     SAQPSEPAASESKPTKPEEPTPAEPAPTTPAPAQEGFTCKGPAVQLFRRGTIAGPNFG
                     YDMTRNGLKGHPELASVDFVEFLLETATLSGKKQEYVLVGIAPDANTAAQLKSVVKSF
                     SPSAGVDIPCPAIAPVRKLQFDVSSPQNPPAAHHNAS"
     gene            complement(2931..4058)
                     /locus_tag="PK710_20675"
     CDS             complement(2931..4058)
                     /locus_tag="PK710_20675"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HOT12204.1"
                     /translation="MRFGQSFEYGLRGCRPSATLTDLLAMSPTLEFRDPPEAIVDADG
                     HVALGAYRRPFRHANLEQARITLGRIPLGRVGSRLVLKEWQHVAIILPEAMLTYAIVD
                     AKYVKTSWCHFVNRDGTGQFEHARKGLRLRVARDLWNATTQVRAHGYRIHVHNHLDVG
                     EHTIRIHIHNDGRAPSVEAELRCMHDLERNPPLEVVMPVGPGRAMYSHKVSLPIEGTL
                     KVGQRVIDVCADEARAIWDVHKAHYPHRTWWNWATFAGKDDQGRTVALNLTRNINRRD
                     DEINENAVWLDGALQRLGPARFTHDPFRSLEPWKIGTADGAVDLSFRPLGERRENLRL
                     GLIRSVFHQPFGVFSGTIHAHGQMVRIDDAWGICEDHDALW"
BASE COUNT          978 a         1220 c         1177 g          887 t
ORIGIN      
        1 gccgacgaac cggaccacaa ggtcaaacga ctggtggtgt atcccggaaa acgtctcagc
       61 ttgcaacgcc atcgccatcg ttccgaacat tggatcgtcc tcgaggggga agcgatggtg
      121 gttcaggacg acgaagaagt gcgcatgctt ctcggtcaat cgatcgagat accgaaaggc
      181 tgttggcacc gcattcgtaa ccctggcatg gaaaacctcg ccattatcga agtgcagacg
      241 ggagagtatt ttggcgagga ggatatcgaa cgaaaagaag acgattttgg acgcgccccc
      301 ccattgtcgc tttgaccgac cccatcgctg caccaacccc atcgaaccta aataatatca
      361 agtaatttca tatagttaca cccataccgc ccctgtcttg accgcagctt tcgtcgttcg
      421 acacaaactt gtccttttcg gcctgtcgga gggagggtcg agtcatgtat cggccaatca
      481 ttcgattcgt ttcgacggct gccgtgatgg tcatgatggg atgcgatcaa cgcgggcccg
      541 ccgccccgag ttcggaaccc acgggaaaca cgacgatgcc cgctcccacg gagcaagcca
      601 cccaagagac tattaccgaa cacgcgaaac ctgctccccc catcgccgag gattccaaac
      661 aagcggcaac gtcgactccc gcgggacgtc gtcccctgaa tgtcattttg ctggtcatcg
      721 atgccatgcg tgatgacatg ccctggaacg gctacgatcg tcccattgcc cccaatctca
      781 cggctttgca cgccaagagc gtaagctacg agcgtggata cgccatctcg tcgttcacgt
      841 ccaaaagtgt cgccggatta ttatccggcc aatatccgtc gtcgttggac cggacaacgc
      901 cattttttac cagttatcga gataagacga acacgttcat ggccgaggtc cttcgagaca
      961 acggtgttcg cacgctgggg attcaagccc atttgtactt gaaagagaaa agcgggttgc
     1021 atcaaggctt cgatgattgg cgcatcgttc caggcatcga atgggatccg aacaaagacc
     1081 cgttcatcac ttctccgcag catacggcct ccgtcatgga acagctttcg gatccgaaga
     1141 acgtacaagg caaatttttc gcgtattacc actatatgga tccccacgat atttataatt
     1201 cgcatgagca agcgccgaag tggggtacga cgccccgtga tcgctatgat gaagagatat
     1261 ggtttgctga ctaccatatt caaaaggcgc tggagttcgt ggggtcacaa ccctggggca
     1321 aagatacggc catcatcgtg acagccgatc atggggaggc gtttggtgag catggttttt
     1381 atcggcatgc gcgccatttg tacgaggtgc tcattcgcgt acctttattt gtgcatattc
     1441 caggcgtgga accgcgaaaa atcgcacgtt ggcgtagcca tatcgacatc gttcccacgg
     1501 tgtatgacat gatgggcgtg cccattccag agggattgcc ggggaccagt ttgcttcccg
     1561 agatattggg agaagaggtg ccgcaacggc ccatcgtcgc cgacctgccc gcggacaata
     1621 tcaattcgcg tcgccgcgcc ctcatcgacg acggctacaa actcgtggcc catggtcagg
     1681 acgttcgatt cgagatgtat gatgttcgca acgatccggc agaaacgacc aatcttttcc
     1741 gaaaggataa ggcgcgggcc cacgcgattc gggagcagta ccgaaaagtg tcggaagcca
     1801 tcccgttcaa gaaggccaga ggtggtccgg cagtcaagaa agattgaagg taggtcgctt
     1861 ctgccgaagc ctgctgcgag cgagcggcag cgcacgatgg ctcgatcctg tgcggaaccg
     1921 tgattcaccc ccaggcgagg ctgcggcatg gttggtcgta cggacgtcct aggaagtcat
     1981 gaaagggtgt gataattggg ttccatgatg aaccagagat gggctgttgc cgggttgatg
     2041 atggcttgcg tgacgattgg ctgcgggggt tcgagtgttc cctctgccaa gtcaagttct
     2101 ggatacgagg aatacacatg cccggagccc attggaaaga tagtccgtga ggattgttcc
     2161 ggcgtggggc ttcgctacga cggcgaacga ttcgaaggtt cggtgggagt cggcggtgtg
     2221 ggtgccagcg cctcctacca gcaaacggca attcgacaag ctgattcact catccagctc
     2281 ctcaaggagc agcgtgttgc gatgtgcaat aacttcaaca cgtgcaagtt gacggtagcg
     2341 gagtaccgag aggatcaaaa acgaatcgat gattctttca tcgcgttgat gtccttgaaa
     2401 gatcgcatga aagacatgga tgcggatcaa gcttcgcagc tccttcgcga gattcaggcg
     2461 attcgaaccg gagtgcagaa cgtggcgtcg gctcaacctt ccgaaccagc ggcgtcggaa
     2521 tccaagccca cgaagccgga agagcctaca ccggccgagc ctgcgcccac gacccccgcc
     2581 ccagctcagg agggcttcac gtgcaaaggt cccgcggtac agctttttcg tcgaggcacg
     2641 atcgcgggtc ccaacttcgg ttacgacatg acgcgcaacg gattgaaagg gcacccggaa
     2701 ctcgcctcgg tcgacttcgt ggagtttctc ctcgaaacgg caacacttag cggcaagaaa
     2761 caagagtatg tcttggtggg tatcgccccg gatgccaaca ccgctgccca actcaaaagc
     2821 gtggtgaaaa gcttcagtcc aagcgcgggc gtcgatatcc catgccctgc catcgcaccg
     2881 gtgcgaaaac ttcaattcga cgtaagctct ccccagaatc cccctgccgc tcaccacaac
     2941 gcatcgtgat cctcacatat cccccacgca tcatcgatcc gcaccatttg accatgagca
     3001 tgaatcgttc ctgaaaatac cccaaagggc tgatgaaata ccgatcgaat cagccccaat
     3061 cgcaggtttt ccctgcgctc tccgaggggg cgaaacgata aatcgaccgc gccatcggcg
     3121 gttccaatct tccacggctc caacgatcga aacggatcgt gcgtgaaacg tgctggcccc
     3181 agcctttgta gcgcaccatc gagccacacg gcgttttcgt tgatctcgtc atcgcgacga
     3241 ttgatgttgc gcgtaaggtt caaagctacc gtccgcccct gatcgtcttt tccggcaaac
     3301 gttgcccaat tccaccatgt acgatgaggg taatgggctt tgtggacatc ccaaatcgca
     3361 cgcgcctcgt cggcgcaaac atcgatgact cgctgaccaa ctttcaacgt cccctcgatg
     3421 ggaagcgaga ctttatgcga atacatcgca cggccaggtc ccaccggcat cacgacttcg
     3481 aggggtggat tccgctccag atcgtgcata cagcgcagtt ccgcctctac cgagggggca
     3541 cgaccatcgt tgtggatgtg gattcgaatc gtatgttccc ccacatccag atgattatga
     3601 acgtgaatgc ggtagccgtg ggcgcgcacc tgggtcgtgg cgttccaaag atcgcgggct
     3661 acccgcagtc gtagcccctt tcgcgcgtgc tcgaattgac cagtgccatc gcgattgacg
     3721 aaatgacacc acgacgtttt cacgtatttc gcgtcaacga tcgcatacgt cagcatggcc
     3781 tcgggaagga tgatcgccac gtgttgccac tctttcagaa cgagccgaga accgactcgt
     3841 ccgagcggta ttcgccccag ggtgattcgg gcttgctcga ggttggcatg acgaaagggg
     3901 cgacgatagg caccaagggc gacatggcca tccgcatcga cgatcgcttc gggagggtca
     3961 cggaactcga gggttggaga catggcaagc aagtctgtca aggttgccga tggtcggcaa
     4021 cctcgaagcc cgtattcaaa ggattgtccg aaacgcacgc cagcttcttg tccgcgccga
     4081 tgactgtgat agcctgcctt ttccgagggc cacggtcatg aaaacgattc acgcggcagg
     4141 ctatttgctc tggctttcgc ttctagtgac gggttgtgaa gagaataaaa caccaccacc
     4201 gcccgtacga acacccgtga cgcctgccgt cgaaccgcct gataccgcgc ctgctttatc
     4261 cg
//
LOCUS       DAOSOR010000512         4258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_20808_length_4258_cov_3.464906, whole genome shotgun sequence.
ACCESSION   DAOSOR010000512 DAOSOR010000000
VERSION     DAOSOR010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20808_length_4258_cov_3.464906"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PK710_20680"
     CDS             complement(<1..395)
                     /locus_tag="PK710_20680"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOT12205.1"
                     /translation="MQSIDLRLYVVTDRGQAGERGVGPIVRAAIEGGATVVQLREKQC
                     DTRDFVRYARELVEICRPARVPLLINDRVDVALAAGADGVHLGQADMSLADARRLLGP
                     SAIVGVSVRHGDEVLAAERDGANYLAANGV"
     gene            complement(399..1217)
                     /gene="thiM"
                     /locus_tag="PK710_20685"
     CDS             complement(399..1217)
                     /gene="thiM"
                     /locus_tag="PK710_20685"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF006830.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOT12206.1"
                     /translation="MTESTQFHALDDDARVRDKAPLIHQITNFVVMNFTANVTLALGA
                     QPVMSHAVQELEEMQGFASALVVNIGTLDDVWIESMLRAARVASDKGVPVILDPVGAG
                     ATRLRTDVARRILDETKVAVVRGNAGEILAGAGDGGQVRGVDSLAEAQEVVGVARRFA
                     EERGVIVAMTGAVDVVTDGVRTYCIENGHPLMGRVTGTGCGSTVAVASYVGVADVEHR
                     CEAAVSALAVYGRAGEIAAEASRGPGTFVPAFLDALYQAPTVMKGQDFRVFRKE"
     gene            complement(1455..4061)
                     /locus_tag="PK710_20690"
     CDS             complement(1455..4061)
                     /locus_tag="PK710_20690"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT12207.1"
                     /translation="MRHVSIIFPVPVIVLGWLAGCGGSEGTADEVSTTDASVDAQGQA
                     GQAGQAGEAGVEDSEAGKVDTGSGGAEPDGSSCVDECESEGMRGCHEGGLRTCGQYDA
                     DSCLEWSDVEVCPDGLVCDPSTVTCREACLDHCDPFSIVLVPDPQNYSSKQDNNASNT
                     YRKQTQWMVDHWKSDNIQMVVHLGDNTNHNEESEWKVGDAAFATLDKAGMPYSMTTGN
                     HDYLVKGAFQRGGSLFDKYFGKSRFEGKPWYGGSYGSSNINNYTTFQVGTMKFLVLSL
                     EFAPRKDVLCWAHQVIANHPDYHVIIATHCYLTHGGGYSGCPDPKYTTTGAAGGTTFK
                     ELASRHSNVFLVVSGHINDSEYRVRKGNLGNDVHQMVVDYQMEGPCAESKAADCKNHC
                     RAGGHTGNGWLRKLVFDPRNNTIQAETFSVEEGNKKVFPGGKPTLFCSELFKPSDPNA
                     TGDNWYASDPNHSDHRFQFAFDMTSPVSYAYDDAGQRAFMDRTVNSVGTGDQLVPRVA
                     LAPTGSHVIVWQDDSSSADGAGNHDIMARGFEPGGCAGFADIVVNQSTAGHQHSPAIA
                     MDSQGNFVVAWVDDPSHNGNSRIRARGFSADGKERFAEFVVSATGTAQQRHPAIAMAP
                     DGNFVIVWEQGPVATAQIAMKGFSANGSERFASQSVHSDVLGQRIHPSVAMDGASNFV
                     VVWQDDSDANGSYQIHARGFHAAGTERFARMTINSVAAGQQRDSVIGMANSGAFVVAW
                     ADDQNKTGNHQILARGFSAQGQQTIADFQVHEKTGKYSNPRIAMEPNGAFVIAWQADG
                     GATAGQAQARSFLANGSEWRPQWKLHRVESGDQLTPDIAINATGSLVATWADDMDGNG
                     AMQILSAGFDSP"
BASE COUNT          944 a         1439 c          994 g          881 t
ORIGIN      
        1 accccattgg ccgcgagata gttcgcccca tcccgttcgg ctgccagtac ctcgtcccca
       61 tggcgcaccg ataccccaac gatggcgctt ggcccgagca aacgacgcgc atccgcaagc
      121 gacatatccg cctgaccgag gtggacccca tcggcccccg ccgccaacgc aacatccacg
      181 cggtcgttga tgagcaatgg cacacgagcg gggcgacata tttcgacaag ttcgcgggca
      241 taacgcacga aatcccgagt atcacattgt ttttcgcgca gttgcaccac cgtggctccc
      301 ccttcgatcg ccgctcgcac gatggggccg acgccccgct cgcccgcctg cccccgatcg
      361 gtcaccacgt agagccgcaa atcgatggat tgcatcgctc actcctttcg aaagacccga
      421 aaatcctggc ctttcatcac tgtgggcgcc tggtagagcg catcgagaaa agccggaacg
      481 aacgttcccg gcccccgcga cgcctccgct gcgatctccc ccgctcgccc atataccgcc
      541 aaggccgaga ccgccgcctc gcaacgatgc tctacatccg ctacccccac ataactcgcc
      601 accgccaccg tcgacccaca ccccgtcccc gtcacccgac ccatcagtgg atgaccattt
      661 tcaatgcagt atgtccgcac cccatccgtc accacgtcca cagcgccggt cattgccacg
      721 atgaccccgc gttcctcggc aaatcgccgc gccacgccca ccacttcctg cgcttccgcc
      781 aacgaatcca cccctcgcac ctggccccca tctcccgctc cggcgagaat ctcccccgca
      841 ttgccccgaa ccaccgccac cttcgtctca tcgagaatcc ttcgcgccac atcggtccga
      901 agccgcgtcg cccccgcacc caccggatcc agaatgaccg gaaccccctt atcgcttgcc
      961 acacgagccg ctcgtagcat ggactcaatc caaacatcgt cgagcgttcc tatattgacg
     1021 acgagcgccg acgcaaaccc ctgcatttct tccagttcct gaaccgcatg agacatcacg
     1081 ggctgcgccc caagggcaag cgtcacattc gcggtgaagt tcatgacgac aaaattcgtg
     1141 atctgatgaa tgagaggcgc cttgtcccgc acccgcgcat catcatcaag agcatgaaat
     1201 tgagtcgatt cggtcattcg tcgctccttt ccatctggtt ctaccctgca ctcggcaact
     1261 ccgtatcaaa ggttgcatcg catcgcaacg ctctctgcaa cctacgcgat cgatgcctcg
     1321 aggtggaggg aatcaaataa cgataccgaa tcagccaaat tatgtagtag atctcgacac
     1381 gccctcaagc gcagccctgc ttctcgggcc ttccgacaac gacagacgcc tcacgatgca
     1441 ttccacagct acgttcaagg gctgtcgaat cccgcggaga gaatctgcat ggcaccatta
     1501 ccatccatat cgtcggccca ggtagccacc aacgaacctg tcgcattgat ggcgatatcc
     1561 ggtgtcaatt ggtccccact ttcgacacga tgcagtttcc actgggggcg ccattcactg
     1621 ccattggcca aaaatgaccg cgcctgcgcc tgtcccgccg tggccccccc atcggcttgc
     1681 caagcaatga caaaggcgcc gttaggctcc atcgcaatcc ggggattcga atatttccct
     1741 gttttttcat ggacttggaa gtcagcgatg gtctgctgcc cttgcgcgga aaaaccgcgc
     1801 gccaaaatct ggtgattgcc ggtcttgttc tgatcgtctg cccaggccac cacgaatgcc
     1861 cccgaattcg ccatgccaat caccgaatcc cgttgctgcc ccgcggccac actgttgatg
     1921 gtcattcgcg caaatcgctc cgtccccgcg gcgtgaaacc cccgtgcatg gatttgataa
     1981 cttccgttgg catcggaatc atcttgccat accaccacga aattcgacgc gccatccatc
     2041 gccaccgaag ggtgaatgcg ctgccccaac acatcggaat gaacgctctg ggaagcaaat
     2101 cgctcggaac cgttcgcgga aaaccctttc atggcgattt gggcggtggc tacgggcccc
     2161 tgctcccaaa cgatgacgaa gttgccgtcc ggcgccatgg caatagcggg atgacgctgt
     2221 tgcgctgttc ccgtcgcact cacgacaaat tccgcgaacc gttctttgcc gtccgccgaa
     2281 aacccacggg cgcgaattcg cgagttgcca ttatggctcg gatcatccac ccaagcgacc
     2341 acgaaattgc cttgagaatc catggctatc gcaggagaat gctggtgacc ggcggtggat
     2401 tggttgacga cgatatccgc gaatcccgcg catccccccg gttcgaaacc gcgtgccatg
     2461 atatcgtggt tgcccgctcc atccgcggac gatgaatcat cctgccatac gatgacatga
     2521 ctgcccgtgg gcgcaagcgc cacgcgggga acgagttgat ctcccgtacc gaccgaattg
     2581 accgtccgat ccatgaacgc ccgctgaccg gcgtcatcat aggcatagga aaccggtgag
     2641 gtcatgtcga aagcgaattg gaaccggtgg tcggaatgat tcggatcact cgcgtaccag
     2701 ttatcccccg tggcattggg gtccgaaggc ttgaacaatt cgctgcaaaa cagcgtgggt
     2761 tttcctccgg gaaatacctt tttattacct tcttccacgc tgaacgtctc ggcctgtatg
     2821 gtattgtttc gaggatcgaa aacgagcttg cgtaaccacc cattgcccgt atgccccccg
     2881 gcacgacaat ggttcttgca atccgcggct ttggactccg cacagggccc ttccatctgg
     2941 taatcgacaa ccatctgatg aacgtcgttg ccgagattcc ctttgcgcac ccgatattcc
     3001 gaatcattga tatgaccgga aaccacaaga aatacattgc tatgccgcga agccaattcc
     3061 ttgaacgtcg tgcctcccgc cgccccggtg gtggtatact tgggatcggg acagccactg
     3121 tatccccctc catgcgtcaa ataacaatgc gtcgcaatga tgacgtgata atctggatgg
     3181 ttggcaatga cttgatgagc ccagcataaa acatcttttc gaggcgcgaa ctccaggctc
     3241 aacaccagga acttcatcgt ccctacctga aacgtagtat aattgttgat attgctgctc
     3301 ccgtaggagc ctccatacca gggttttcct tcgaaccggc tctttccaaa gtacttatcg
     3361 aatagactcc cccctcgctg gaacgccccc ttgacaagat aatcatgatt gcccgttgtc
     3421 atgctataag gcattcccgc cttgtcgagc gtggcaaacg ccgcatcccc caccttccac
     3481 tcggattcct cattatgatt agtgttatcc cccaaatgaa cgaccatctg gatgttgtcg
     3541 ctcttccaat gatccaccat ccattgcgtc tgcttacgat aggtattgga agcattatta
     3601 tcttgtttgg aagagtaatt ctgaggatcg ggaaccagca cgatcgaaaa aggatcgcaa
     3661 tggtccagac aggcctcgcg gcaagtcacc gtagaaggat cgcaaaccaa cccgtctgga
     3721 catacctcca catccgacca ctccaaacag gaatccgcat cgtattggcc acacgtacgc
     3781 aggcccccct cgtgacaacc ccgcatcccc tcactttcac attcatcgac acacgacgaa
     3841 ccatccggct ccgccccacc cgatcccgtg tcgactttcc ctgcctcgga atcctccacc
     3901 cccgcttctc ccgcctgccc tgcttgccct gcctgcccct gggcatccac ggaagcatcc
     3961 gtcgtcgata cctcatcggc cgtcccctcc gaaccgccac atccagcaag ccaacccagc
     4021 acgatgacgg gtacaggaaa aatgatcgag acgtggcgca tggcagctct ccgatctgca
     4081 caaaaaaaac cgcaatgatt gctttttgtt caagacttca cggtaaagtc cgaaacagag
     4141 tcgatagtag catgagaacc agaaaaaatc ccgccgatag cccttcggta gcttctcggg
     4201 gatcagtgca taacctattg atttttataa ttttttattc aagctagcca cgtggagc
//
LOCUS       DAOSOR010000513         4254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_20826_length_4254_cov_4.288164, whole genome shotgun sequence.
ACCESSION   DAOSOR010000513 DAOSOR010000000
VERSION     DAOSOR010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20826_length_4254_cov_4.288164"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="PK710_20695"
     CDS             <1..1176
                     /locus_tag="PK710_20695"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOT12208.1"
                     /translation="VFLRGMTDVEMVALTEAMLDSGKVLDLSHIPGIKVDKHSTGGVG
                     DKVSLALVPIVAACGVPVPMVSGRGLGHTGGTLDKLEAIPGFNVNLDADAFARIIREV
                     GGCMIGQTAEVAPADKRIYALRDVTATVESVPLIVASILSKKLAEGIDALVLDVKVGK
                     GAFMKDLEQARTLASALVRVGTRAGKRVVALLTRMEAPLGRTIGNALETREAIDVLFE
                     RAPADMTECTYALCAEMLLLADAASDRDHALRRISEVIRSGKAAQVMENMIAAQGGNP
                     KVVRDPSLMAAAPHVVPVLAPRSGFVCEMDALEMGLSAVMLGAGRTRADQSVDPAVGI
                     VLNAHLGEKVEKGSILAHVHVRDVQAAKPAMDRILHAFKLGDEAKSPEPLVIERIES"
     gene            1221..2489
                     /locus_tag="PK710_20700"
     CDS             1221..2489
                     /locus_tag="PK710_20700"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT12209.1"
                     /translation="MDSVAPWDLGGVVHTVIRAWDYGDPSEPTLLSALAMSLRQVAGS
                     DKIALIIEREGKGWVLGPYDDARVDPADDQITKAHDSILDLDSIPSVPVCVENPDPDN
                     SLERALLEIGIDCFTLIPFVDTRPLGAIVLGHPAGKAPVEAFAQGAEVLAILGKRVGS
                     TIRLRQKGYLPGGSDVLRAMALIEKKAMLGAFAGGLIHEINNPATFMGLAGCQIEKLV
                     KERCAEQSTFEMLFDLADGIRDATHQIRNLVDAFRLLSGSTKQQLVVMVDLERVLDAA
                     VILTRAAHRYDAVIETDIEAIPPCPGQYVELASVLVALLMNALEAMDASTQSKVIQVR
                     ARARERVMEIRIRDTGAGMTKETLARAFEPFFTAKNSRQHAGLGLTMARQTVERFGGT
                     IRMESEKGKGTTVWIQIPLCTETVPPSSPS"
     gene            complement(2494..3987)
                     /locus_tag="PK710_20705"
     CDS             complement(2494..3987)
                     /locus_tag="PK710_20705"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT12210.1"
                     /translation="MTKAEDFQPMLLQSLEPRPKRMTFNRLGEGKDGGPPEPASMGSA
                     VYTFDSTFVPRDAHVEALANLRSFIAELSGAQQAYLLLNGTTSGNLGMVLATVRPGQK
                     IIIPRNAHKSVLGGVVLSGAAPVFIQPEISAELNLVFTIKPEQIRQALLDNPDATAVL
                     LTNPTYQGFCLDLAETERVVHQSGKLLLIDEAHGPHFRFHPDLPLSAMRAGADIAVQS
                     THKILGPFAQTSVLLVNSQRVDDIRLRMAMTMITPSKISHLLAGSLEAAMTQIAEDGE
                     AILQRVIDMTENARDRINQIPGVRAIGREVIGKSESVVDVDLTKLVIDVAGLNLSGYR
                     AARVLHDDYGVSAEMAGATTVTMVVTLGNDQKDLDRCVTGVEQLSRRQRSARAQSSPL
                     LHILALQTPPMGRQVLVPRDAFFARKARVPVPESTGKISGESVIPYPPGIPVLVPGEE
                     ITPEIVEYLTSVRRTGIEIHGCEDRSISTLRIVDEREFLHNFGSGWG"
     gene            complement(3984..>4254)
                     /locus_tag="PK710_20710"
     CDS             complement(3984..>4254)
                     /locus_tag="PK710_20710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12211.1"
                     /translation="APRRNVKLGESVGFVCAEAIHHHTTGLPLVFPGEEITSEMVDYI
                     ISEGARDATVFTSNDQSLQQVGVVDERKMILHRSFFRGAAAWGGR"
BASE COUNT          899 a         1139 c         1264 g          952 t
ORIGIN      
        1 gtttttcttc gcggcatgac cgatgtcgag atggttgctc ttaccgaggc catgctcgac
       61 tcaggaaagg tccttgacct gtcacatatc cctggcatca aggtggacaa acactccacc
      121 ggtggcgtgg gtgataaagt atctcttgcg ctcgtgccca tcgtggccgc ctgtggtgtt
      181 cctgttccca tggtttccgg aagagggctc ggccacacgg gcggaacact cgacaagctc
      241 gaggccattc ccggcttcaa cgtcaacctc gacgctgacg ctttcgcacg catcattcgt
      301 gaggtgggtg gatgcatgat tggtcagacc gcggaggtgg ctcccgcgga caagcgcatc
      361 tacgcgttgc gcgatgtgac ggccacggtg gaatccgttc ctctgatcgt ggccagcatt
      421 ctgtccaaaa aactggccga gggtatcgat gcgctggtgc tcgacgtcaa agtcggcaag
      481 ggcgcgttca tgaaagatct cgagcaagcg cgcacgctgg cttcggcgct ggttcgtgtg
      541 ggcacgcgtg cgggaaaacg tgtcgttgcc cttctgacgc gaatggaggc gcctctcgga
      601 cgcaccatcg gcaatgcact cgaaactcgg gaggcaatcg atgtgttatt tgaacgagca
      661 cccgcggaca tgaccgagtg tacgtatgct ttatgcgcgg agatgctgct tcttgccgat
      721 gccgcaagcg acagagacca tgccctgcgt cggatttccg aagtcatccg aagcggaaag
      781 gccgcacagg tcatggaaaa catgatcgcc gcgcaggggg gaaacccaaa agtcgtccgc
      841 gacccgagcc tgatggctgc cgcgccccat gtcgttccgg tgcttgcccc gcgatcgggt
      901 ttcgtttgcg aaatggatgc tctcgagatg ggcttgtccg ccgttatgtt gggcgcaggt
      961 cgaacgcgag ccgatcaatc ggtggatcca gcggttggaa ttgtcctgaa tgcccatctc
     1021 ggagagaaag tagaaaaagg ttctatcctc gctcatgttc acgttcgcga cgtgcaagcg
     1081 gcgaaaccgg ccatggaccg aatccttcat gcgttcaaac taggcgatga ggcgaaatct
     1141 ccagagcctc tggtgatcga acggattgaa agctgataca atacgctcac gggtcatcgg
     1201 aacgctctgg aggagatgcg gtggattctg tagctccgtg ggatcttgga ggtgttgttc
     1261 acaccgtcat acgagcgtgg gattacgggg atccgtcgga acccaccctc ttatccgcgc
     1321 tcgcgatgag tcttcggcaa gtggccggca gcgataaaat agcattgatc atcgagcgcg
     1381 agggaaaggg ctgggtgttg ggcccttacg atgacgcaag ggttgaccct gcggacgatc
     1441 aaatcacgaa agctcacgat tcaatcctcg atctcgattc gataccgtcg gttcccgtat
     1501 gtgtggagaa tcccgatccg gataattctc tcgaacgagc gctgctggag attgggattg
     1561 actgtttcac gctgataccc tttgtggata cgcggccttt gggagcgatt gtcttgggtc
     1621 atcccgcggg aaaagcgcct gtcgaagctt tcgcgcaggg agccgaggtt ctggccatcc
     1681 tgggaaaacg agtgggaagc acgatacgtt tgcgtcagaa gggctatctt cccggcggat
     1741 ccgatgtgtt gcgcgccatg gctctcatcg agaaaaaagc catgcttggc gctttcgcgg
     1801 gaggattgat ccacgagatc aacaacccgg cgacgttcat gggtttggct ggatgccaga
     1861 tcgaaaaact ggtgaaagag agatgcgcgg aacaatccac gttcgagatg cttttcgatc
     1921 tcgcagatgg cattcgtgac gccacccacc agatccgtaa cctcgtggat gcatttcgcc
     1981 ttctttcggg atcgacgaaa cagcagttgg tggtcatggt cgatctggaa agggtgcttg
     2041 acgcagcggt cattctcacg cgtgcggccc atcgttatga cgcggtgatt gagacggata
     2101 ttgaggccat tccgccgtgt ccggggcaat atgtcgaact tgcgtccgtg cttgtggcct
     2161 tgctgatgaa cgcgctcgag gccatggatg cttcgacgca aagcaaggtg attcaggtgc
     2221 gtgctagagc acgcgagcgg gtcatggaga ttcgaattcg cgacaccggg gcagggatga
     2281 cgaaagagac gttggctaga gctttcgaac cttttttcac agcgaaaaat tcgcggcagc
     2341 acgctggact cgggctcacc atggcacggc aaaccgtgga gcgattcggc ggaaccattc
     2401 gcatggaaag cgaaaaagga aaaggaacga ccgtatggat tcagattccg ctttgtacgg
     2461 agacggttcc gccctcttcc ccatcgtgag ccatcatccc catccgctgc caaaattatg
     2521 caaaaactcg cgttcatcga cgatccgtag tgtggagatg ctgcggtctt cacacccatg
     2581 gatttcgatt cccgtgcgtc gtaccgacgt cagatactcc acgatctcgg gcgtgatttc
     2641 ttcaccggga acgagtacgg ggatgcctgg gggataagga atgacggatt ctccgctgat
     2701 ttttccggtg gattctggga cggggacgcg ggcttttcgt gcaaagaatg cgtctcttgg
     2761 cacgagcact tgccttccca ttggaggggt ttgcagtgcg agaatgtgta gaagagggct
     2821 ggattgcgct cgagcgcttc gttgtcgtcg cgatagctgc tcgactcccg ttacgcagcg
     2881 gtccaagtct ttttgatcat tgcccaacgt gaccaccatg gtcacggtgg tagctcccgc
     2941 catttccgcg cttacgccat aatcatcgtg caacacgcgg gcggcccggt acccggacag
     3001 gttcagaccc gcaacatcga tgaccagttt ggtaagatcc acatccacca cgctttcact
     3061 tttcccaatc acttctcgac cgatggcgcg aacgcccgga atttgattga ttcgatcccg
     3121 ggcgttttcg gtcatatcga tgacccgttg caggatggct tccccatctt cggctatttg
     3181 ggtcatcgcc gcttccaagg atccggcaag aagatgagag atcttcgatg gcgtgatcat
     3241 cgtcatggcc atgcgcaatc tgatgtcgtc gacccgttgg ctgttgacga gcaacaccga
     3301 agtttgtgcg aaaggaccta ggattttatg cgtgctttgg accgcgatat ccgctccagc
     3361 gcgcatggcg ctcagcggca gatcgggatg aaaacggaaa tgaggtccgt gggcttcatc
     3421 gatgagcaga agttttccag attgatgcac gacgcgttcg gtttccgcca aatcgaggca
     3481 aaagccttgg taggtagggt tggtgagcaa cacggcggtc gcgtccggat tgtcgagcag
     3541 agcttgtcgt atttgttcgg gtttgatggt gaacaccaag ttgagttccg cggatatttc
     3601 cggctgaatg aatacggggg ccgcacccga taacaccaca cctccgagta cggacttatg
     3661 cgcgttgcga gggatgatga tcttttgccc aggacgaacc gtggcgagca ccatgcccaa
     3721 gttgccgctc gtggtgccat tgagaagaag ataggcctgt tgcgctccag acaattcggc
     3781 aatgaaactc cgcagattcg ccaatgcctc cacatgagcg tcacgaggga cgaaggtgct
     3841 gtcgaaagtg tatacggcac tgcccatgct tgccggctct ggagggccgc catcttttcc
     3901 ctcgcccaaa cgattgaacg tcatgcgctt gggacgcggt tcgagggact gaagcaacat
     3961 tggttgaaag tcttcggcct tcgtcatcgt ccaccccatg cagcagcgcc gcgaaagaag
     4021 ctacggtgca aaatcatctt tcgttcatcg accactccca cttgttgcaa ggattggtcg
     4081 ttgctcgtaa acacggtggc atcacgcgcg ccttcggaga tgatgtaatc aaccatctcg
     4141 ctcgtgatct cttcacccgg aaataccaag ggaagacccg tggtgtggtg atgaatcgcc
     4201 tcggcacaaa cgaatcctac cgattcgccg agtttgacat tgcgcctcgg cgca
//
LOCUS       DAOSOR010000514         4251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_20853_length_4251_cov_3.573403, whole genome shotgun sequence.
ACCESSION   DAOSOR010000514 DAOSOR010000000
VERSION     DAOSOR010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20853_length_4251_cov_3.573403"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..1300)
                     /locus_tag="PK710_20715"
     CDS             complement(50..1300)
                     /locus_tag="PK710_20715"
                     /inference="COORDINATES: protein motif:HMM:NF017893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HOT12212.1"
                     /translation="MKRLLAIGVIWLGCLIAWVILGSTIVFRSDQSSMNRYDDVALLW
                     GPALQQEPPSAVYTETKKVRESRTTTGMDGTAVVTDVEKQVEQAFPLTLAATDIQTSL
                     HLEHRRKGLLWFPTYTVDFSARYAFVNETDAERRTIVRFPLGQTNTIFEGFGVQTGDG
                     QPVEVQIEAGVAWWNVSFAPGQRREYKIAYRSRGTNSWHYQLTAGTGRVNDFRLTMQT
                     NFSEIDFPAGTLSPSEHGVEGDGWKGTWQFNTLVANAPIGVSLPQLLNPGPLASKITF
                     FAPVGLLFFFFVVAVFAAAHGKNLHPLHYFYFGCAFFAFHLLFAYLVDHVAIAPAFAI
                     ASGVSILLVVTYARLFVGWKYAAMFMGIPQLLYLVLFSFTFFWEGFTGLSITCGAILT
                     LFVMMQFTGRLSWRGLSGTPNSNF"
     gene            complement(1888..3405)
                     /locus_tag="PK710_20720"
     CDS             complement(1888..3405)
                     /locus_tag="PK710_20720"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOT12213.1"
                     /translation="MAESPAEPVQSAAQLTDQADAVASASAQPGQESKPRPEEPRIYS
                     KRRHVWIFYEPNASGGWTGFLGLGSSVRLKSTKSRVGDGCEAFYPVEPRGWVCLNHKT
                     TLDPNDAEYKAILKYSPKIDRPFPHQYGESRGTPRYNEIPTREQQFQREYQLAEHLQA
                     VEKLRAGQLPENEIPKSLRGVDVSLAGSGPPPELSLFPRGIQESRDYLKPLSTVAWSH
                     EFDAEGRTWLVTADLALVPKDKVTPYPKSDFGGVLLDENTRLPIAFTRYEARRKYRRG
                     AEGQMQPTDQMWPRMSILPIDDDKVEVQGKTYYHVKATDDWMDTEDATVARLSPVTPW
                     GAPVQGARVDAPRDPHAQTVKAPEGGRKTWVEVSVLGGWMVAYENTKAVFATLIAPGR
                     GGIPTKGVDPLQTASTPVGTFRVDGKFFTSTMANPAFVHSDVPFAQNFHGPHVLHQAY
                     WHDGWGEKRSAGCINLSPKDALWFFHWTEPMIPEGWYGMRSDKDAGSATVVWVHR"
     gene            complement(3552..>4251)
                     /locus_tag="PK710_20725"
     CDS             complement(3552..>4251)
                     /locus_tag="PK710_20725"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOT12214.1"
                     /translation="NAISMAENYIYIEDQYFRMPMVNDLIVERMKAKPNLRLIVITKP
                     VSEWTDPGCAWTHASHQLFKSNFPNRYMMLQLRAFDYVDVGWGWDETEVRWGDFDVHS
                     KLMIIDDKFLSVGSCNKNNRGLVYEGELNVAVLDAAWVREARRRVFANILPEGTAPTD
                     DVSTWWGQFEAAAAWNDTVFQNWKDEGNDISLDGAPLPAKYRPEGFVFSLEFRTVSDC
                     LIESVGPDMVGRDD"
BASE COUNT         1021 a         1310 c         1068 g          852 t
ORIGIN      
        1 ggttcttacg ccccggtcct aatgtggaac gaagaaagga tctgtagtac tagaaattcg
       61 aattaggggt gccagaaaga cccctccacg acaaccgccc ggtaaactgc atcatgacga
      121 aaagtgtcag gatggctccg caggtgatgg atagccccgt aaaaccctcc cagaagaagg
      181 taaacgagaa aaggacgaga tagaggagct ggggaattcc catgaacatg gcggcatatt
      241 tccatcccac gaacaagcgc gcgtaggtaa cgactagcaa gatggatact cccgaggcaa
      301 tggcgaaggc gggggcaatc gccacgtggt ccaccagata cgcaaatagc aggtggaagg
      361 cgaaaaaggc gcaaccaaag tagaagtaat gcagtgggtg gaggtttttc ccatgcgcag
      421 cggcgaatac cgccaccacg aaaaagaaga aaagcaaccc gacaggcgca aaaaacgtga
      481 ttttcgaagc caaaggcccg ggattgagca attggggtaa ggacacacct atcggtgcat
      541 tcgccaccaa cgtgttgaat tgccacgttc ccttccaccc atccccctcc accccatgct
      601 cgctcggtga tagcgttccc gccgggaaat cgatctccga aaagttggtc tgcatcgtca
      661 gccgaaagtc attcacccgt cccgtccccg ccgtaagctg gtaatgccac gaattcgtac
      721 cccgggaacg atacgcaatc ttgtattccc ttcgctgccc aggcgcgaaa gaaacgttcc
      781 accacgccac ccctgcctcg atttgcacct ccacgggctg accgtccccg gtttgcactc
      841 caaacccctc gaaaatcgta ttggtttgtc ccagggggaa acgtacgatg gtacgacgct
      901 cggcatccgt ttcgttcacg aacgcgtagc gtgccgaaaa gtccacagta taggtgggaa
      961 accataacag ccccttacga cgatgttcca ggtggagcga tgtttggata tccgtcgccg
     1021 ccaacgtgag agggaaagcc tgctccacct gcttttcgac gtccgtcacc accgccgtgc
     1081 catccatccc tgtcgtcgtg cgagattctc gtaccttttt cgtttccgta tacaccgccg
     1141 atggcggctc ctgctgcaac gccggccccc aaagcaacgc cacgtcatcg taacgattca
     1201 tggaggattg gtccgaccga aacacaatcg tcgagccgag aatcacccag gcaatcaaac
     1261 aaccaagcca aatcacgcca atcgcaagca gccgtttcat ggaacacctt cctgccaaat
     1321 cgtggagata agccccatcg tacaaaaacg atatgacact tccgtcggga ctccaaggat
     1381 aaggtcggga ggaattgtgg cgaaatgatg gcgtgacccc tgccaccgtc gtatcgcccc
     1441 cctcgccccg gtcgcgtttg ccttggcccc acacggccca cggctcacgg tcacggccac
     1501 ggcctgcgcg aaacggtcac ggtccacgag aaagggtcac ccaaacttga tgactctagc
     1561 cgttcgcttc ccatgtagat attcctagga ggcgcatcac caaagccatc aaagaaaacc
     1621 cgccagaaat cgggaggcca cgctccacgt atcgaaaaat gccgaagcgc cgaaagacaa
     1681 atccctcacc gagcttctgg agaaagcttc gtaacccgca agtatcggcc cggcgtattc
     1741 aactacatgt atttactaat gtttttataa ggggtggtca aaaggggccc aaagtcaaag
     1801 ttcagggcat tgtcgctcgt atgcctttct cattcaggga aaaatgaggg aatctatcag
     1861 gttctacacg cctttctccc ttccccacta ccgatgaacc cataccaccg tcgcagaccc
     1921 cgcatcttta tccgaacgca tcccgtacca cccctccggt atcatcggct ccgtccaatg
     1981 aaagaaccat aacgcatcct taggagataa attgatacac cccgcactcc gcttctcacc
     2041 ccagccatca tgccaataag cttgatgtaa aacatgcggt ccatgaaaat tctgggcaaa
     2101 cggtacatca gaatgcacaa aagccgggtt ggccatcgtc gaggtaaaaa acttcccatc
     2161 gacccggaac gtacccacgg gcgtcgacgc cgtttgcagc ggatccaccc ctttggtggg
     2221 gattcctcca cgccccggcg caatcaacgt cgcaaaaacc gctttcgtat tctcatacgc
     2281 caccatccat ccccccaata cactgacttc cacccaggtt tttcgtcctc cctcgggagc
     2341 tttcaccgtt tgcgcatgag ggtcgcgcgg cgcatcaacc cgagccccct gtaccggcgc
     2401 cccccacggc gtcaccggcg aaagcctcgc caccgtcgca tcctcggtgt ccatccaatc
     2461 gtccgtggcc ttgacatgat agtaggtttt cccctgcacc tcgaccttat cgtcgtcgat
     2521 aggcaaaatc gacatccgag gccacatctg gtcggtgggt tgcatctgtc cctcggcccc
     2581 ccgacgatac ttgcgtcgcg cttcgtaacg cgtgaaagca atgggaagac gcgtgttttc
     2641 gtcgagcaat acgcccccga agtcggattt ggggtaaggg gtcaccttgt ctttgggcac
     2701 gagggcgagg tcggcggtga cgagccaagt gcgcccttcg gcatcgaact catgggacca
     2761 cgccaccgtg gacaagggtt tgagatagtc cctcgattcc tggatacccc gcgggaaaag
     2821 agagagttcg ggaggcggac ccgagcctgc aaggctgaca tcgaccccgc gcaacgattt
     2881 ggggatctcg ttttctggca attgccccgc gcgcaatttc tccaccgcct gcagatgctc
     2941 tgccaattga tattcccgct ggaattgctg ctcccgggtg ggtatctcat tgtaccgagg
     3001 cgtaccccgg gattcaccat attggtgcgg aaagggacga tcgatcttcg gggaatactt
     3061 gagaattgct ttatattccg catcgttagg gtcgagcgtg gtcttgtggt tcaagcaaac
     3121 ccaccccctt ggctccaccg gataaaaggc ttcgcatcca tcacccaccc ggctttttgt
     3181 gctcttcaac cggaccgaac tgcccaaacc aagaaatccc gtccaccccc cggaagcatt
     3241 gggctcgtaa aaaatccaaa catgccttcg tttggagtaa attcgtggct cttcgggccg
     3301 tggcttcgac tcttgtccag gttgggccga agccgatgca acggcatccg cttggtccgt
     3361 aagctgcgcc gccgactgca ccggctcggc aggtgattcg gccataggct cggaggaacc
     3421 tttgccgttg cacccaagta agcagggaac cagaaaaata ccaaaccgca aaaaggaaac
     3481 cacgagaaga acctagcgaa aaatgtccgc ggggggaatg gctcccgacc cgcgctcgtc
     3541 atccccaccg atcaatcgtc gcggcccacc atgtcaggcc ccacgctctc gatgagacaa
     3601 tcggatacag tgcggaattc caaggaaaag acgaatccct cgggcctgta tttcgccggc
     3661 agcggcgccc catccagact gatatcattg ccttcgtctt tccaattctg gaataccgta
     3721 tcattccagg cggccgcggc ctcaaattgt ccccaccacg tcgacacatc gtcggtaggc
     3781 gcggtcccct caggcaggat gttggcgaaa acacgacgac gcgcctctcg aacccacgcg
     3841 gcgtcgagca cggccacgtt caactcccct tcatacacaa gcccacgatt gtttttgttg
     3901 caggacccca cgctgaggaa tttatcatcg ataatcatca gcttggagtg aacatcgaag
     3961 tctccccagc gcacctcggt ttcatcccac ccccagccga cgtccacata gtcgaaagca
     4021 cggagctgca acatcatgta gcgattaggg aaattggatt tgaaaagctg gtgggaggca
     4081 tgcgtccacg cacatccagg gtcggtccat tcgctgaccg gcttggtaat cacaatgaga
     4141 cgaagattcg gttttgcttt catccgctcc acgatgaggt cattgaccat gggcatacgg
     4201 aagtattgat cctcgatata gatgtagttt tccgccatcg aaatggcgtt g
//
LOCUS       DAOSOR010000515         4249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_20864_length_4249_cov_3.976395, whole genome shotgun sequence.
ACCESSION   DAOSOR010000515 DAOSOR010000000
VERSION     DAOSOR010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20864_length_4249_cov_3.976395"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..281)
                     /locus_tag="PK710_20730"
     CDS             complement(18..281)
                     /locus_tag="PK710_20730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12215.1"
                     /translation="MAAMIKYSSLFLVACALAFLGCSDDEDSGKVCTADEETACTTAY
                     TTCSSEAAANADMQACNQCIEDYCKCYSNCGNTCDKSKLTPCE"
     gene            425..1105
                     /locus_tag="PK710_20735"
     CDS             425..1105
                     /locus_tag="PK710_20735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12216.1"
                     /translation="MPMSKLHHRAIPLALCLGSAFGCDSLVEKPPDPYPIVIVVESDP
                     GKPIEAAGILVGTHQVGATDETGKAHLKLRGDEGESISLSVKCPEGHQNPKPVAVTLR
                     RIADPNARPEYRLSCAPQTRTIAVAIRAEEGPNLPVVFLGREIARTDASGAAHVSMQM
                     RPDETFQLMLKTSETEAGRSLRPQDPVATFRVKNHDEVVTFNQEFKVERRTIRWSRPK
                     GPTRIDTR"
     gene            1254..1643
                     /locus_tag="PK710_20740"
     CDS             1254..1643
                     /locus_tag="PK710_20740"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HOT12217.1"
                     /translation="MAIRIVRLGTPRAKGEPLRVGTVRRPPRGVPKNEFASRDFYDVW
                     FPTVAPTQQTMDIAHSATSPADWSRFFKQYRSEMKKPDAKHAIDLLAAMSHVVDFSIG
                     CYCENELHCHRHELRNLLRAAGAHILE"
     gene            2146..2688
                     /locus_tag="PK710_20745"
     CDS             2146..2688
                     /locus_tag="PK710_20745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12218.1"
                     /translation="MKQRLASRDLAYCGLFGAAALLLPILFHAVHLGHVFMPMYLPLV
                     CLGFFARPVPTAVTALLSPLLSAAIFGMPPFFPPVASSMALELATMAGLIAWLKQRWP
                     NANEWLILVPVLLLGRVLYVVFVYATALLIELPPKFMAGLSLLGGWPGLILMVIVVPP
                     VARAGKAIAYPVAKGEKKEP"
     gene            2688..3305
                     /locus_tag="PK710_20750"
     CDS             2688..3305
                     /locus_tag="PK710_20750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT12219.1"
                     /translation="MHSKVEFFNNIAELWDGWEDRDALGCVMKAGLQKMEISSHEIIL
                     DVGCGTGNLTQALLGRLGPGAKVAAIDISPRMVEVAKTKVRDGRVSWQVADACQTPFS
                     SNYFDRIFCFSVWPHFDDPKATALELRRVLRPLGRLHIWHHIGRDKVNEIHATASPAV
                     SRDILLPAQDLAALLLEWGFDVLEAEETENHYLISAQKPEKTGEC"
     gene            3299..4042
                     /locus_tag="PK710_20755"
     CDS             3299..4042
                     /locus_tag="PK710_20755"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOT12220.1"
                     /translation="MLALEEVWGSAKGRVRRLAPQTRIASGVLLFSGCMVLPASTGMG
                     AIVLVVASLGWSLYCQPPKRILRAALVLGLMLFSPYFLLIPVMAEQLSLSAFSAAARV
                     VAGIFAHGMAGMVVSITTVTSLTVSDLREGLLRLPVPSLVAVIVLQIVHQSLVLVEET
                     RRIASAMSVRGACGRGMTAFRVLVSLPQVWLPRVIQRAERVAAAMDVRGYCAQDLPSF
                     ENHRVGTRDGGVVVMGMGFLILAIMIRIQ"
     gene            4051..>4249
                     /locus_tag="PK710_20760"
     CDS             4051..>4249
                     /locus_tag="PK710_20760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT12221.1"
                     /translation="MCDALGYDRVTVGFQPQGPPVLDSLCFQVGQSERVALVGLNGSG
                     KTTLLMATVGLLEHQGTIRVCG"
BASE COUNT          853 a         1316 c         1117 g          963 t
ORIGIN      
        1 tgctttccgc cgcttcttca ttcacaaggc gtaagcttgc tcttgtcaca ggtgttgccg
       61 caattcgaat aacacttgca gtagtcttca atgcattggt tgcacgcttg catgtcggca
      121 ttggccgccg cttccgaact acaagtcgtg tacgccgtcg tgcaggccgt ttcctcgtcc
      181 gctgtgcaaa ccttgccaga atcctcatca tcgctgcaac cgaggaaagc caaagcgcac
      241 gctaccaaaa atagactcga gtattttatc attgcggcca tatatctcac tatttcgcga
      301 aagcaagatc gaaaatacct tggacgaaaa atgttcgaac agagattgtg tccaggctaa
      361 ccctcgaacc caatcatctc cgccctactc cacccctgct caccgtgcta tactcaccct
      421 gccgatgccc atgtcaaagc ttcaccaccg cgcgatcccc ttggcccttt gtcttggctc
      481 cgcctttggt tgcgattcgc tcgtggaaaa accaccggat ccttacccca tcgtgatcgt
      541 ggtcgaaagt gatcccggaa aacccatcga agccgcgggt attttggtcg gcacccacca
      601 ggtcggtgcc accgacgaaa ccggcaaagc ccacctcaaa ttgcgcggcg atgagggcga
      661 atccatctcc ctttcggtca aatgccccga aggacaccaa aatcccaaac ctgtagccgt
      721 gaccttgcga cgtatcgccg accccaacgc acgcccggaa taccgcttgt cctgcgcccc
      781 ccaaactcgc accatcgccg tggccatccg cgccgaggaa ggaccaaacc tgcccgtggt
      841 gttcttggga cgggaaatcg cccgaaccga tgcctccggt gccgcccacg tcagcatgca
      901 aatgcggccc gatgaaactt ttcaactcat gctaaaaaca tccgagacgg aagccggacg
      961 ctctttgcgc cctcaagacc ccgtcgctac ctttcgcgtg aaaaatcacg acgaagtcgt
     1021 caccttcaat caggaattca aggttgaacg tcgcaccatt cgctggtcca ggccaaaagg
     1081 ccccacacgt atcgataccc gctgaattga accatctccc ccccgcccct tttcaaaaaa
     1141 ttccaccgaa ccacgccccc tcgagccccc cgcggcttgc ggacgcccct cgtgtttctt
     1201 atcaagctcc tataaacgcg cgaaacgctt gctccctgcc caggaaatac aacatggcca
     1261 tccgcatcgt tcgtttagga actcctcgcg caaaagggga acccctgcgc gtgggaaccg
     1321 tgcgacgccc gccgcgaggc gtgcccaaaa acgagttcgc aagccgcgat ttctacgatg
     1381 tctggttccc cacggtcgct cctacccaac agaccatgga tatcgcccat tcagccacga
     1441 gccccgcgga ctggtcccgg tttttcaaac aatatcggtc cgaaatgaaa aagcccgacg
     1501 ccaaacatgc catcgacctt ctggccgcca tgtcccacgt cgtcgacttc agcatcggat
     1561 gctattgcga aaacgaattg cactgtcatc gacatgaact gcgtaatttg ttacgcgctg
     1621 ccggggctca cattctggaa tgagccaacg gcttccttgc ttacctggct cccttttcgc
     1681 tatccaacat gctggcccgt atcagttacc cctctgcggt tacccttccc accgccatca
     1741 accatcgagg ctcctctcct cgaaatacac cctgaccaag gatttcgggg tggtatcgag
     1801 cttccagggc gtcgagagcc tcctgcagcc ccgagaacgt gcccaccctc gccaaaaacg
     1861 aactccgcct ccctcactct caccgttgcc gagcggcaac aagcaccagg ctagtccggc
     1921 tcccttgacg atcccttgcg gcttcggtag cacggtcctt ggaattgatg ttactaatgc
     1981 actgttccaa ccccttttcg ccaaccgata gcgtggcagg gcttttgggg aaagaagtcg
     2041 gaaacggctg cccccttatt ggcgctcttg gccatccctg gacatcaccg gcgataaact
     2101 cgaacagcag gtggagccat tgcatgcgtg cattggaaaa tacagatgaa acaacgtctg
     2161 gcctcccgtg atctcgcgta ttgtgggctt tttggggccg ctgccctgct tttgcccatt
     2221 ctctttcatg ccgtacacct cggccatgtc ttcatgccca tgtaccttcc gctggtctgt
     2281 ctcgggtttt tcgctcgacc tgtgccgacg gccgtgaccg ctctcctcag tcccttgttg
     2341 tccgccgcca tcttcggtat gccgccgttt tttccacccg tggcctcgag catggccctc
     2401 gaactcgcca ccatggccgg cctcatcgcc tggctcaaac aacgatggcc gaacgccaac
     2461 gaatggctca tcctcgtccc tgtcttattg ctcgggcgtg tcctttatgt cgttttcgtc
     2521 tacgccaccg cgctgctcat cgaacttccc ccgaaattca tggcggggct gtcgctgttg
     2581 ggaggctggc cgggactgat cctgatggtg atcgtggtgc cgcccgtggc tcgggccgga
     2641 aaagccatcg cttaccccgt cgccaaggga gaaaaaaagg agccataatg cattccaaag
     2701 tggaattttt caataatatc gctgagttgt gggatggatg ggaagaccgg gatgccctgg
     2761 gatgcgtgat gaaagctggg cttcaaaaaa tggagatctc ttcccacgaa atcattctcg
     2821 atgtcggatg cggcaccgga aacctcaccc aagcgctgct tggtcgtctg ggacccggcg
     2881 caaaagtggc tgccatcgac atctcgccac gcatggtcga agtggccaaa accaaagtgc
     2941 gtgatggccg ggtgtcgtgg caagtggcgg atgcgtgcca aaccccattt tcatccaact
     3001 atttcgatcg catcttttgc ttttccgtct ggccccattt cgacgatccc aaagccacgg
     3061 ctctcgaatt gcgacgcgtg cttcgacctt tgggacgttt gcatatttgg caccatattg
     3121 gccgggataa ggtcaacgag attcacgcta cagcgtctcc cgccgtgagc cgggacattc
     3181 ttcttccggc acaagacctt gcggcgctat tgctcgagtg gggatttgac gttctcgaag
     3241 cggaagaaac ggaaaatcat tacctcatct ccgctcaaaa gccggaaaag acaggcgaat
     3301 gctagcgctc gaggaggtgt gggggagtgc caaaggcaga gttcgacgtc ttgccccgca
     3361 gacgcgaatc gcctcgggcg ttttgctgtt ttccgggtgc atggttttgc ccgcatcgac
     3421 tgggatggga gcgatcgtgc ttgtggtggc gagcctggga tggtcgttgt attgccagcc
     3481 gccgaagcga atccttcgag cggccctggt tttggggctg atgctatttt ccccatattt
     3541 tttactgatt ccggtgatgg ccgagcaact atccctgtcg gcgttttcgg cagcggcacg
     3601 agttgtcgcg ggaattttcg cgcatggcat ggcgggcatg gtcgtatcga tcacgacggt
     3661 gacgagcttg accgtcagcg acttgcgaga agggctgctt cggttgccgg ttccttcgct
     3721 cgtagccgtt atcgtgttgc agatcgttca tcaaagtctg gtattggtcg aagaaactcg
     3781 acggatcgct tcggccatgt ccgttcgggg cgcgtgtgga agaggcatga cggcttttcg
     3841 cgtccttgtg tctctcccgc aagtgtggct gccacgggtc atccagcgcg cggagcgggt
     3901 tgccgcggcg atggacgttc ggggctattg cgcccaggac ttgccatcgt tcgaaaacca
     3961 tcgggttgga acccgggatg ggggggtagt cgtgatgggg atggggtttc tgatactggc
     4021 aattatgatt agaattcaat aggttgggtg atgtgcgacg cccttggata cgaccgcgtg
     4081 accgttggct ttcagcctca aggccccccc gtcctggatt ccctttgttt ccaggtggga
     4141 caaagcgagc gggtcgccct cgtcggactc aatggctccg gcaagactac cctgctgatg
     4201 gcaaccgtcg gcctgctcga acaccaagga acgattcggg tctgcggtc
//
LOCUS       DAOSOR010000516         4244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_20900_length_4244_cov_4.223204, whole genome shotgun sequence.
ACCESSION   DAOSOR010000516 DAOSOR010000000
VERSION     DAOSOR010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20900_length_4244_cov_4.223204"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="PK710_20765"
     CDS             <1..1200
                     /locus_tag="PK710_20765"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT12222.1"
                     /translation="VRRFMREAKAATAIRHPNVIEILDVVEAEGGEPVMVMELLDGTP
                     LDQLMERSGPLALGDVARIMTPVVSAVGAAHALGIIHRDLKPENIYLARATDGSPLPK
                     VLDFGIAKVLDPNQINELATKSGATRTGSMLGTPHYMSIEQACGEKDIDHRTDIWSLG
                     IILYSMLSGHRPYDGENYGQILRALMTTSPPPIEKLVPKLPTEVVDIVNRCMARSRDE
                     RPEDLREVFQVLSRYTQDTTTPAPSLVPIPMSREEMMSGATLQAASVYVPKSGGGSVH
                     QLANRRMMLVVGVGAAFLLLGGSLGAALFFRAEGNGPSEPAAASAEQVQLMAPATQST
                     EVNPVVALVGSQEEPMASASAAISSSPPPQRTGKRTDPQPTHSAVTPATAAPTPKGGG
                     IIETNPY"
     gene            complement(1465..3066)
                     /locus_tag="PK710_20770"
     CDS             complement(1465..3066)
                     /locus_tag="PK710_20770"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOT12223.1"
                     /translation="MTPFRLCLIGLLLTACSDSQESPSDDPGVFDAGMDSTDSATNDD
                     VQADAQADAPFDGASTCGELGSTRCVGVWVETCEPTGWSEPVECPLDQGCRDDACQEP
                     TTRQVQQAASVQQLIDTLSRYSGWHEPVDASAVKERERRAILKGDGSDQTYFASAWRA
                     MNAYPQGHQSLYSLEPGVCGTLLRWQATSRFGVCARPVQEGLIITFAKDGNPLGLHAG
                     DVITHADGVGGDAMLATAYLQPVCGSVFPSAAGRRHAAAASFFGTVETATTLRVRTPE
                     GKVHDVIVSDVEDTATTDCTDPFARNRWVYAETTIRSDGVAVIRLPSFFPFDKEFPTD
                     PKDFDAFIEAYQNEMVKIFEPAKSAKAIVWDARGNAGGITPVALAIIGGFPSARPTSV
                     SYCRSRVVGSSPPSFDDYRYAIYEVTPGGPFSYSGKVAIVVDGLAYSAGDYFPFAALQ
                     ASDAVVVGSPSAGAFGGGHGPIPLAGPPALEANFDPAACFDHASNGFLEANPASPTVE
                     IEYDPADLAKGRDTLLERAIQQLEL"
     gene            3370..3852
                     /locus_tag="PK710_20775"
     CDS             3370..3852
                     /locus_tag="PK710_20775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12224.1"
                     /translation="MGFVMLVGCGGGKPEPSTPASTSDYDEMATESESEPEETSASSH
                     SSAPVSEMGERPPPIPEAWELHQRDCDALGAKYEQLLLAVELEKLEARKLNEKQRGAA
                     EQNVRGTAKQGAENWMRACEGIVGTVQVKSRWDCAYRANTLDRFKGCMDGKYDREGDD
                     "
     gene            complement(3895..4074)
                     /locus_tag="PK710_20780"
     CDS             complement(3895..4074)
                     /locus_tag="PK710_20780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12225.1"
                     /translation="MDEVAWTALIVMPLGLTIPSSTQLAGRQQLRLILRSVLTLGSFR
                     LGELPAPIEQPLPYN"
BASE COUNT          937 a         1133 c         1338 g          836 t
ORIGIN      
        1 gttcgccggt tcatgcggga agccaaggcg gccaccgcga ttcgtcatcc caatgtgatc
       61 gagatcttgg atgtggtgga agccgagggt ggcgagccag tgatggtgat ggagctattg
      121 gatggaacgc ccctcgacca actgatggag cgttcgggac cgttggctct cggtgatgtg
      181 gcgcgaatca tgacacccgt ggtttcagcg gtgggggctg cgcatgcctt ggggatcatc
      241 caccgcgatc tcaagccgga aaatatttat ctcgcgcgcg caaccgatgg cagccctctt
      301 cccaaagtgt tggatttcgg gattgcgaag gtcttggatc ccaatcaaat caacgaactc
      361 gcaaccaaaa gcggcgccac acgtacgggg tcgatgcttg gaacaccgca ctatatgtcc
      421 atcgaacaag cttgcggtga aaaggatatc gatcatcgga cggacatctg gagtcttggg
      481 atcattttgt acagcatgct cagtgggcat cgaccgtacg acggggagaa ctatgggcag
      541 attcttcgag cgctgatgac cacaagtcca ccgcccatcg aaaagctggt tcccaagtta
      601 ccgacggagg tcgtggatat cgtcaatcga tgcatggcgc gttcgcgtga tgagcgtccg
      661 gaggatcttc gggaggtttt ccaagtcctg tcgcggtaca cgcaagacac caccacacca
      721 gcgccatcgt tggttcccat accgatgtcg cgagaggaga tgatgtccgg ggcgacgttg
      781 caagcggcaa gtgtctatgt tcccaagtcg ggtggggggt cggtgcatca gctagcgaat
      841 cggcgaatga tgctggtggt tggtgtggga gcggcgtttc tgttgctggg gggaagtctt
      901 ggggcagcgt tatttttccg ggccgagggc aatggcccaa gcgagccagc cgccgcttcg
      961 gcagagcagg tgcagctcat ggctccggct acccaatcca cggaagtcaa tcctgtggtc
     1021 gcgctggtcg gttctcagga agaaccgatg gcaagtgcct ctgctgccat ttcctcttcc
     1081 cctccgccgc agcggacggg caagcgtacg gacccccagc cgactcattc cgcggtgaca
     1141 cctgccacgg ctgcccccac gcccaagggc ggtggcatca tcgaaaccaa tccttattga
     1201 gggcatgaaa cgggtttcag gctgcatcga acgtgctgct cattcgaatg cgaagggcgt
     1261 cgggatcgat tcctagctat ccatatacgt actattcact ccagcaagat atccagcgaa
     1321 ttcaccggga tcaacacagt tatcttattg attatattgc atttttcaga tttgtttcaa
     1381 gcaagattgt ggatcatgga ggggaagcgt ggggggtaga gccccgttca acttatacat
     1441 ataaggatag ccctccggtc gatatcaaag ctcgagctgt tggatggctc gttcgagcag
     1501 ggtgtcgcgc cctttggcaa gatcagcggg atcgtattcg atttccacgg tggggctggc
     1561 agggttggct tccaaaaagc cgtttgatgc atgatcgaaa caggcggcgg gatcgaagtt
     1621 ggcctcgagg gcaggcggtc cggcgagagg gatgggacca tgaccgcccc cgaacgcgcc
     1681 ggcggaaggg gaaccgacca ccaccgcatc cgacgcttga agggctgcga aggggaagta
     1741 gtcccctgca ctgtaggcga ggccatcgac aacgatggcg acttttcccg agtaggagaa
     1801 aggtcccccc ggcgtgactt catagatggc gtatcggtag tcatcgaacg aagggggaga
     1861 ggaaccgaca acccggctgc ggcagtagga aacggaggtg ggacgggcac tggggaatcc
     1921 gccgatgatg gcgagagcga cgggagtaat tccaccggca ttgccacgcg catcccaaac
     1981 gatggctttg gcggactttg ctggctcgaa gattttcacc atctcgtttt ggtaggcttc
     2041 gatgaacgca tcgaagtcct tggggtccgt ggggaactcc ttgtcgaagg gaaagaaact
     2101 gggcaaacga atgaccgcca ccccatcgga ccttatggtg gtttccgcat acacccatcg
     2161 atttcgcgcg aatggatcgg tgcaatcggt cgtagccgta tcctcgacgt cggagacgat
     2221 gacgtcatgc accttcccct cgggcgtgcg cacgcgcagg gtggtggccg tttccaccgt
     2281 accgaaaaag gaagccgccg ccgcgtgacg ccgacctgcc gcggaaggaa atacggagcc
     2341 gcataccggt tggaggtacg ccgtcgcaag catggcgtcg ccaccaaccc catccgcatg
     2401 ggtgatcaca tcgcccgcgt gaagccccaa gggatttcca tccttcgcga acgtgatgat
     2461 gagtccctct tgcacggggc gagcgcatac accaaagcga gacgtggctt gccagcgcaa
     2521 gagcgtccca cacacgccgg gttcgaggga atacaggctt tgatgtcctt ggggataggc
     2581 gttcatggca cgccaggccg atgcaaaata ggtttgatcc gagccatctc ctttcagaat
     2641 ggcgcgtctt tcccgctctt tgacagccga cgcatcgacc ggttcatgcc agccggagta
     2701 tcgagaaagc gtatcgatga gttgctgcac cgatgcggct tgctgcacct gtcgcgtcgt
     2761 gggctcctga catgcgtcat cgcgacatcc ttgatcgagc ggacattcca cgggttcgct
     2821 ccaaccggtg ggctcgcagg tctcgaccca cacgccgaca catcgtgtcg acccaagttc
     2881 gccacaggtc gacgcaccat caaacggggc atcggcttgg gcatcggcct ggacatcgtc
     2941 gttcgtcgcg gaatcggtcg agtccatccc cgcatcgaaa acgccaggat cgtcggaggg
     3001 ggactcctgg ctgtcagaac aggcggtgag gagcaaaccg atgaggcaaa gtcgaaaagg
     3061 ggtcatggca gtagcccgac agcaatcggc ataccaactg taaggcgttg atattatgtg
     3121 catttaatct atgtcgcccc aacctggcac gcccagggcg tcacgacaat gacacaaaaa
     3181 gcggatggga tatcggtggt cgccgcgtga tgggcgacgg aggaagcgac ctgttttcgt
     3241 gggtgccgtg actgcctgtc gagggtttca ccgttcgcgt catggctatg aagcaccgtt
     3301 caattccttt tccaaggagc gaaacatcgt cgtattctgg ggcctatgga ccggaataca
     3361 gcgtggatca tgggtttcgt catgctcgta gggtgtggag gcggcaagcc ggaaccatcc
     3421 acacccgcat cgacgtcgga ctacgatgag atggcgacgg aatcggaatc ggagccggaa
     3481 gagacctcgg catcgagtca ttcctcggct cccgtgagcg agatgggaga acgtcctccc
     3541 cccattccag aagcgtggga gcttcatcag cgggattgtg atgctctcgg cgccaagtac
     3601 gagcaactat tgctggcggt ggagctggaa aagctcgaag cgcgcaagct caacgaaaag
     3661 cagcggggag cagcggaaca gaatgtgcgc ggcacggcca agcagggagc ggaaaactgg
     3721 atgcgagcat gtgaaggcat tgtgggaacg gttcaggtca agagccgatg ggattgtgcg
     3781 tatcgggcca acaccctcga tcggttcaaa ggctgcatgg atggcaagta cgatcgggag
     3841 ggtgatgatt gaggtggcgg gaggagggta tcgctttcta ttttcccaac gatttcaatt
     3901 gtaaggcaac ggctgttcta tcggggccgg gagttcgccg aggcgaaagg acccaagcgt
     3961 gagaacggag cggaggatga gccgaagctg ctgtcgtccc gcgagttggg tcgatgacgg
     4021 gatggttagc cccaagggca tcacgatgag ggcggtccaa gcaacctcat ccatgacgag
     4081 cggggaacta gcatatcgtt gaggaatccg tcgatgacga cccctacctc ccaacttatt
     4141 gatattaaac gtttttttgg agagtccgtg gacggaaaac ccaagaagag cagccggtca
     4201 aagggtccag acaatcacgt caagcaccga tacggctgga aagt
//
LOCUS       DAOSOR010000517         4225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_21036_length_4225_cov_3.605276, whole genome shotgun sequence.
ACCESSION   DAOSOR010000517 DAOSOR010000000
VERSION     DAOSOR010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21036_length_4225_cov_3.605276"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..774
                     /locus_tag="PK710_20785"
     CDS             166..774
                     /locus_tag="PK710_20785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12226.1"
                     /translation="MRPRTPFALMTTLLLAWAHPANAQTPPSSDFGLRGGLLGSTSYF
                     APIGASAGVFGHYFIDPSLRLGAGVDALSIKSTSSDRLDYDGQSSQALRLSVRGEHHP
                     WPESTVDPWLGLALGSFYGERQAALDGRSAETGFGLVASAELGLAVWCTSAFALQAAF
                     SLDIPLTHTEVLGGDGRVGRGTFFSGAGSVLPLPLLGLRFAL"
     gene            962..1777
                     /locus_tag="PK710_20790"
     CDS             962..1777
                     /locus_tag="PK710_20790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12227.1"
                     /translation="MGLLDCYARAFGLWLLLTTALGCCGPNYEQVLEKHRAAVEKKIA
                     PLPSIHKQLLSLPALQKDEVNLVGAAVDINLSGSNTLSGNTLLCHAEDFAVADELGYV
                     PIRLPTTGKLNQCSSILHRNHDVFDPAKPSAPLLRPFGFSAQSDFERCEAAQNLLILR
                     ILEVVEPSVPVAAASSFQPNPTICDPAASVAPASSTAMKHIRPMTFSGGRISAEALFF
                     SLDDARYLGGFRVQASSSSNLKGTDVQLDFSTQLRKALAEGVRKHIPGATVRE"
     gene            1796..2419
                     /locus_tag="PK710_20795"
     CDS             1796..2419
                     /locus_tag="PK710_20795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12228.1"
                     /translation="MSHAPWLSDKARATVLDQIVGLEATSGAELVVTVAVRSGHYRHA
                     DYLVGALLSLAALLFYLFYPDPLFDDVAVMLIIASFGVGTILGASLSPLRRVFVSRKQ
                     MDEEVRKAAHSKFVEQGISRTRDRTGILVYVSLFEQRVEVVPDIGIPVQDLGERWASG
                     TKALDISAKNGFAAFAQALAAMGPMLTEAVPRAPDDINELPDDMVQA"
     gene            2416..4122
                     /locus_tag="PK710_20800"
     CDS             2416..4122
                     /locus_tag="PK710_20800"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HOT12229.1"
                     /translation="MRIRSPSWRGWNAGLWRASCLMIGLCLCLHAQARPGGGSGFSGG
                     GSSSGGGSRSSGSSGGGSSRSSSSPSKGSSSSSSIETFSYCIENGAARPDALRTWQPG
                     SLGPFGAGPPRPAELPVADASDVAISATIIFLIGFAVLGAPVLLLGGVVFLVGRRLGS
                     GWSTTSSPTARLRTPTRQPGEVRRSLAILRTWDTDFSVALLDDFAYALYAEAHTARGT
                     GALDRLAPYLGPSARATLARQGQRPVSAVIVGAMRYTAFDPNANEGNAVRLTIEFESC
                     YTETAGPGTSQSYYAMETWHFIRSRVARSRPPDRIRVWTCPSCGAPLDRIMGSTCTYC
                     QRVVDGGAFDWTVDSIVVNERSARPPILTGTTEEQGTELPTVFDPHVQEALGSLQHKD
                     PTFSQEALFARINMIFHTMQTAWSSLAWDTARPYLTDNLFQTNSYWISAYRAQKLRNI
                     TEKARIVHIEIVRLESDRFFDALTVRLHATGLDYTLRDTDGALVGGSRTQERPYTEYW
                     TVVRSAERQGAARGTASCPNCGAPMNITMAALCQHCGVKVNSGEFDWVLSRIEQDEVY
                     GG"
BASE COUNT          828 a         1405 c         1095 g          897 t
ORIGIN      
        1 caaggaccga gccaaactca acacccaaga atctttagcc cgtatccctt tccagttccg
       61 gaaaaaatga agggatccgt cagcatatca ccgcccccac gacaatccac acggcggtat
      121 cttggcccgg ttaatgctct cgatgagaga aacaaggaga aaaccatgcg ccctcgaact
      181 cccttcgccc tgatgacgac tctgctgctc gcgtgggctc accccgccaa cgcgcagact
      241 cccccctcat ccgatttcgg actccgcggc ggactccttg gctccacgtc ctatttcgct
      301 ccgattggcg cctccgcggg cgtcttcggc cactatttca tcgatccctc gctacggctc
      361 ggagctggtg tcgacgccct cagcatcaaa tccacatcca gcgaccgtct cgattacgac
      421 gggcaatcct cccaagccct tcgtctttct gtgcgcgggg agcatcaccc gtggcccgaa
      481 agcacagtgg acccgtggct cggactcgcg ctgggaagtt tttacgggga acgacaagcc
      541 gccttggacg gacgttccgc cgaaacaggt ttcggcctcg ttgcctccgc tgaactcggc
      601 cttgccgttt ggtgcaccag tgcttttgcc ctacaagccg ctttttccct cgatattccc
      661 cttacccaca ccgaagtgct cggcggcgac ggacgtgtgg gcaggggtac gttcttctct
      721 ggtgccggct ccgtccttcc gctgcctctt ttgggtctac gttttgccct ctaacgatag
      781 aaatctgtac tatcaattgg ttatgttcca aaccccaaag cgcgaccgct ctccactttt
      841 ttttcaagaa cgtgatatcg taaataaccg ctaattccct gatcggtttt gacatagggc
      901 gaattgacga atcatccctt ccctctttac cacccccgcc ccgacggtgt atcgtgaccg
      961 tatgggcttg ttagactgct atgcacgtgc tttcggcctt tggctcctgc ttaccacggc
     1021 cttgggctgc tgtggtccaa actacgaaca ggtattggaa aagcatcgcg ccgctgtcga
     1081 aaaaaaaatt gccccccttc cctcgattca caagcaattg ctttccttac ccgcattgca
     1141 aaaagatgag gtcaatctcg ttggcgcggc ggtcgacatc aacctgtccg gctccaacac
     1201 cctgtcgggc aacacactgc tctgccacgc cgaagacttc gccgtggccg acgaacttgg
     1261 ctacgttccc atccgcttgc ccaccacggg caaactcaac caatgcagct cgattcttca
     1321 ccgaaaccac gatgtcttcg accccgccaa accttcggcc ccgctgcttc gtcctttcgg
     1381 tttttccgcc caaagcgatt tcgaacgatg cgaagccgca caaaacctgc ttatcctgcg
     1441 catcctcgaa gtcgtggaac cgtccgttcc cgtcgccgcc gccagctcct tccagcccaa
     1501 cccaaccatc tgtgacccag cagcatccgt cgccccggcc tcttccaccg ccatgaaaca
     1561 tattcgcccc atgacgttct cgggaggcag aatctccgcc gaagcgctct ttttctccct
     1621 cgacgacgcc cgctatctcg gggggttccg cgtccaggcc tcgagcagct ccaatctcaa
     1681 aggcaccgat gtccaattgg atttttccac gcagttacgc aaagcgctgg ccgaaggggt
     1741 ccgaaaacac atcccaggcg ccaccgtccg cgaatgaccc acaagggagg tatccttgag
     1801 ccacgcacca tggttgagcg acaaagcaag agccaccgtg ctagaccaga tcgtcggtct
     1861 ggaagccacc agcggagccg agctcgtggt gaccgtcgcc gtccgctcgg gccattatcg
     1921 ccacgccgat tatctcgtgg gcgctcttct ctccctggca gcgctgctgt tttatctttt
     1981 ttaccccgac ccccttttcg atgacgtggc cgtgatgctc atcatcgcaa gtttcggcgt
     2041 ggggacgatc ctaggtgcgt ccctatcacc cctgcgtcgt gtctttgtat ctcgaaagca
     2101 aatggacgaa gaagtgcgca aagcagccca ttccaaattc gtcgaacaag gcatttcgcg
     2161 tactcgtgac cgcacgggca ttctggtcta cgtatccctg ttcgagcagc gggtagaggt
     2221 ggtgccggat atcggaattc ccgtgcaaga cctgggagaa cgctgggcaa gtggcacgaa
     2281 agcacttgat atttcagcaa aaaatggctt tgcggcgttt gcgcaggcgc ttgccgccat
     2341 gggacccatg ctcaccgaag ccgttcctcg ggcccccgac gatatcaacg aacttcccga
     2401 cgatatggtg caagcatgag gatccgatca ccatcgtggc gggggtggaa cgcgggacta
     2461 tggcgcgcgt catgcctgat gattggctta tgtctatgcc tgcatgcaca agcccggcct
     2521 ggcgggggaa gcggattcag cggcggaggc tcgagttccg gcggcggttc ccgttcgtcg
     2581 ggtagttccg gcggaggaag cagccgaagc agcagttcac caagcaaggg aagcagcagc
     2641 tcatcgtcca tcgagacgtt ctcctattgt attgagaacg gagccgcgcg accggatgcg
     2701 ctgcgaacct ggcaacccgg ctcgctcggt cctttcggcg cgggcccccc tcggcctgcc
     2761 gaactgcctg tggctgacgc ctccgacgtg gctatcagcg ctaccattat atttctcata
     2821 ggattcgctg tccttggcgc gcccgtcctg cttctggggg gcgtcgtatt tctcgtgggc
     2881 cgacgtttgg gctcgggctg gagcacaacc tcttccccaa ccgcccgatt acgcacaccc
     2941 acccgccaac cgggagaagt ccgacgctcc ctcgccatct tgcgtacatg ggataccgac
     3001 ttttccgtcg ccttgctcga cgacttcgct tatgcccttt acgcggaagc ccacacggcg
     3061 cgagggacgg gagcgctcga ccgtctggcc ccttatctcg gcccttcggc ccgggcaacc
     3121 cttgcgcggc agggacagcg acccgtttcc gccgtgatcg tcggcgctat gcgctatacc
     3181 gcattcgatc cgaacgcaaa cgaaggaaac gcggtgcgtc ttacgataga gttcgaatcc
     3241 tgctataccg aaaccgcggg ccccggaacc tcacaatcgt attatgccat ggaaacatgg
     3301 cacttcattc gctcccgggt cgctcgatcc cgcccccccg accgcattcg cgtatggact
     3361 tgtcccagtt gcggtgcccc cctcgaccgc atcatgggaa gcacctgcac ctactgccaa
     3421 cgggtggtgg acggaggtgc tttcgactgg accgtcgaca gtatcgtcgt aaacgaacga
     3481 agcgcccgtc cgccgattct caccggcacc accgaggaac aaggaacgga gctacccacc
     3541 gtattcgatc cgcatgtgca agaggcgctc ggcagtttac agcataaaga tcccaccttc
     3601 agccaagaag cgttgtttgc ccggatcaac atgatctttc acaccatgca aacagcctgg
     3661 tcgtcgcttg cgtgggatac cgcgcggccc tacttgacgg acaatctctt ccagaccaat
     3721 tcctattgga tttccgccta tcgcgcccag aagctccgca atatcacaga aaaagcgcgt
     3781 attgtgcata tcgaaatcgt tcgtctggaa tccgaccgct ttttcgatgc tttgaccgtc
     3841 cgtctgcatg ccaccggact cgattacacg ctccgggaca ccgatggcgc cctggtcggg
     3901 gggagccgta cccaagaacg tccctatacg gaatactgga ccgtcgtgcg cagcgccgag
     3961 cggcaaggcg cagcccgggg aaccgcttca tgccccaact gcggcgcgcc catgaatatc
     4021 accatggctg ctctgtgcca acactgtggt gtcaaagtca actccggcga attcgactgg
     4081 gttctgtccc gcatcgaaca agatgaagtg tacgggggct gatacctgag ccaccccctc
     4141 ttttttcgct ggcctttcct tcacggccac ggtcacggcc acggtcacgg tcacggccca
     4201 cgagaaacgc tcacggtaat cccct
//
LOCUS       DAOSOR010000518         4219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_21080_length_4219_cov_4.124880, whole genome shotgun sequence.
ACCESSION   DAOSOR010000518 DAOSOR010000000
VERSION     DAOSOR010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21080_length_4219_cov_4.124880"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..2415)
                     /locus_tag="PK710_20805"
     CDS             complement(184..2415)
                     /locus_tag="PK710_20805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12230.1"
                     /translation="MARGKWSIAPEHLDETLRALQEISDIEGVALLLHTQDGWLPLLE
                     LQANDTQIHEWKEPVDAISIEGSISTTLYFLDGVEPGPVRVQHDEQVDGILLWIGEKP
                     VVAVAGPRMSRLPEGLLHLEGAELSWSSLELAASQVGNSLRSLDVGGNEALGSRAPSL
                     ASSFPKLEGFDLRGIESIQDLTPLANLSQLRRLNLEGCSSLSDLAALSSLSTLTHLDL
                     SDCSAIESLAALRSLTSLISLDLSGCDRLQSLEPLVGLSTLSLLGLADCAQLPDLAPL
                     ARVPSLSSLDLSGCSSLLSVQSIRDCPNLHTLSVLGCSRITDLHVLTACSPLRILEAD
                     DDVMVARVLAGCAVARVDPDYVRQRIESWFALVDRPADGRDLAMDLLPALSLGFSEPW
                     AVSALHDLIDRVKRSGIEDQGLWTRIFISLRLVGDPGWRPSIERALEDLVLGDDSLSI
                     LEPALEALGQISNDSFAGPWARTQATLALDSLIGTPQARRVAKVAVKYYACSRNLSGL
                     DAWIEVLSASSPQQQPASHQTLTSARASESDFTVLAPLVARLAQKQPDSPEMRQVLAV
                     IVGMVRDRPEALSVDPLVVAFEELIEKKPDCAAVGRLWKAFERLLLTAAPASIIAAAA
                     DTILHLIRKNPDNMAFEPLMQEMMDWVVEHPQSPVVERFTALLDAARDSGLVSFSHTV
                     LSHATLRAHASEELREYVSAFDLRQARRAMIRGILDELCAQDIVRNKPRAQIAAAIEE
                     DFG"
     gene            complement(2517..3485)
                     /locus_tag="PK710_20810"
     CDS             complement(2517..3485)
                     /locus_tag="PK710_20810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOT12231.1"
                     /translation="MADVLVVAELAGGQIRKATHSAITFAREAAGHLGGGFSILVIGQ
                     GAAAAAAQLTDFGATKVYTTEITSVNGYVCEHYAPTVAELAKQFAVVVATATAYGKDL
                     IPRVAAKLGAGYAADISGVSHENGLMYKRPIYAGNAFGYVQISTPIQAVTVRQSEFTA
                     AQPAGGASPVENVAAVAPSAGADRVEFISFDQVKSERPELTEASVVVSGGRACKEKFF
                     EVLNPLADLFGAAIGATRAACDAGYAPGDLQVGQTGKIVAPDLYFAFGLSGAIQHLAG
                     MKGSKVIVAVNKDPEAPIFSVADYGLVADLFQVIPELVQQIKAVKE"
     gene            complement(3507..>4219)
                     /locus_tag="PK710_20815"
     CDS             complement(3507..>4219)
                     /locus_tag="PK710_20815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOT12232.1"
                     /translation="FDEYAVEAALRLTENGAQNKVRAGEVVVVTLGPKECETTLRAML
                     ATGADRAIRIEADDDQLDGNVVALALKAIVDKEKPDFVLMGKQAVDGDTNQVGQFLAE
                     YLGWPMATAAATIVEEAGALHVGREVDGGVINLKVKAPGLVTVDLRITAPTSVYSSQT
                     KRDFKYNDGVRFAPLPAIMKAKKKPLDVLKLGDLVADTSLRVIYKKFEPPQGRKAGVK
                     VKDVAELVDKLKNEAKVI"
BASE COUNT          949 a         1226 c         1198 g          846 t
ORIGIN      
        1 acccgacaat agcacacctt gctccgatac gggactcgta tgctcctacg gaacagtatt
       61 gtgttattgt gttatcatgt gggtatgtca gtagtcccgc tatcaaaccc ataggtcgtc
      121 gtccttgggg gataggagca acacctgcgc ccgaccgatc tcaggcctag aattctccgt
      181 ggatcagccg aaatcctctt cgatggcggc ggcaatctga gcacgtggtt tgttgcgcac
      241 gatatcctgc gcgcacaatt catccaaaat cccccggatc atggctcgcc gtgcctggcg
      301 aagatcgaag gcgctcacat attcccgcag ctcctcgctg gcatgagcac gaagggtggc
      361 atgggatagt accgtatggc tgaaagatac caggccggag tcccgagccg cgtcaagcaa
      421 agccgtaaac ctttcgacta cagggctttg gggatgctct acgacccaat ccatcatctc
      481 ctgcataaga ggctcgaacg ccatattgtc gggatttttt cgaatcaaat gaaggatggt
      541 atcggcggcc gcagcgatga tcgaggcagg tgcggctgtg agcaacaatc gctcgaacgc
      601 tttccacagt cgacccaccg cggcgcaatc cggttttttt tcgatgagtt cttcgaaggc
      661 aacgaccaac ggatcgacgc tcaacgcttc cggcctgtcg cgcaccattc cgacgatcac
      721 cgcgaggact tgccgcatct cgggactatc tggctgtttt tgcgccagcc gcgcgaccaa
      781 aggcgcgaga actgtaaaat cgctttccga agctcgcgcc gaggtaagtg tctgatggga
      841 agcgggttgt tgttgtggcg atgatgccga aagcacctcg atccaggcgt ccaatccgct
      901 caaattccgg gaacaagcat aatacttcac cgcgactttc gcgacacgac gggcctgagg
      961 cgttccgatc agggagtcga gagccagggt agcttgcgtg cgagcccagg gcccggcaaa
     1021 cgaatcattc gagatttggc caagggcctc caacgccggc tcgagaatcg acaaggaatc
     1081 atctcccagt accaagtctt ccaacgctct ttcgatactg ggacgccagc cagggtcccc
     1141 caccaatcga agggagatga agattctcgt ccacaacccc tgatcctcga tgccggaacg
     1201 ctttacgcgg tctatcaaat catggagtgc cgaaaccgcc caaggctcgg aaaatcccaa
     1261 ggacaaggcg ggcaacagat ccatcgccaa atcccttccg tctgccggtc gatcgacaag
     1321 agcaaaccag gattcgatac gctgccgcac gtaatccgga tcgacacgag caaccgcgca
     1381 gcccgccaac acgcgcgcga ccataacgtc gtcgtcagct tccaggatgc gcaggggaga
     1441 acaggcggta agaacgtgaa ggtccgtgat gcgagaacac cccagcacgg aaagggtgtg
     1501 aaggttgggg caatcgcgaa tgctttgcac cgaaagcaag gatgaacatc cgctcaaatc
     1561 gagcgaactg aggctaggca cgcgagccaa cggtgccaag tccggcagtt gtgcgcagtc
     1621 agcgagaccg agcaaggaaa gagtcgaaag cccaaccaac ggttccaggc tttgcagtcg
     1681 atcgcatcct gataagtcca gcgaaataag gctggtcaac gagcggagag ccgctaggga
     1741 ctcgatcgcg gaacaatccg aaaggtcgag gtgggtcagg gtggaaagag aagacaaggc
     1801 ggcaaggtcg cttagcgagg agcatccttc caaattcaag cgacgcaact gggatagatt
     1861 cgccaacggt gtgaggtcct ggatcgattc aatcccgcgc aggtcgaagc cttccaactt
     1921 cggaaaggaa gaggcaaggc taggagcccg ggaacccaac gcttcgttgc cgcccacatc
     1981 caggcttcga agactgtttc ccacctggct tgcagcaagt tcgagcgacg accatgacaa
     2041 ttcggcgcct tcaaggtgca aaagaccttc cggaaggcgg ctcatccggg ggcccgccac
     2101 cgcgacaacg ggcttttctc caatccagag caagattcca tccacctgct cgtcgtgttg
     2161 aacacgtacc ggccctggct cgacaccatc gagaaaataa agtgtcgtgg agatggagcc
     2221 ttcgatactg atagcatcga cgggttcctt ccactcatgg atttgcgtat cgtttgcctg
     2281 caattccaac agaggaagcc agccatcctg ggtatgcagc aacagcgcta cgccttcgat
     2341 gtcggaaatt tcttgtaatg ctcgaagggt ttcatcgagg tgctccggag ctatcgacca
     2401 tttgccgcga gccatgattg atccttcctg cgctgaacga tgaacaacgt atcagcgaag
     2461 cggtttctta ccgcatcgac gctacgggaa caacgagggg aggaaggtcg gcgaggttat
     2521 tctttaacag ccttgatttg ctgtacgagt tcgggaatca cctggaacag gtccgccacc
     2581 aggccatagt cagccacgga aaaaatcggg gcttcggggt ctttgttgac ggcaacgatg
     2641 accttggagc ctttcattcc ggcgaggtgt tggatggcac cggacagacc gaacgcaaag
     2701 tacaggtcgg gggcgacgat ctttccagtt tgcccgacct gcaagtcgcc gggcgcatag
     2761 ccggcatcgc aagcagcgcg ggttgcgccg atagcagcac caaaaaggtc ggccaggggg
     2821 ttgagcactt cgaaaaactt ttctttgcag gctcgcccgc ccgacacgac cacggaagcc
     2881 tccgtgagtt ccggacgttc gcttttcacc tggtcaaaac tgatgaattc gacccgatcg
     2941 gctcccgcgg aaggagccac ggccgctacg ttttcgacgg gcgatgcccc ccccgcgggt
     3001 tgagcagccg tgaattcact ctgccgaacg gtgaccgcct gaatcggggt ggagatttgc
     3061 acataaccga aggcgttacc ggcgtagatg gggcgtttgt acatgagccc attttcgtga
     3121 ctcacacctg agatatccgc ggcgtatccc gcgcccagtt tggcggcaac gcgcggaatg
     3181 aggtcttttc cgtatgcggt ggccgtggcg accaccacgg cgaattgttt ggcgagttcc
     3241 gcgacggtag gggcgtaatg ctcgcatacg tagccgttaa cggaagtaat ttcagtggta
     3301 tagaccttgg tggctccgaa gtcggtgagc tgtgcggctg cggcggcagc gccttgaccg
     3361 atgaccaaga tggaaaaccc gccaccaaga tggcccgcgg cctcgcgggc gaacgtgatt
     3421 gccgagtgag tcgcctttcg gatctgacca ccggcgagct ctgcaactac caatacgtct
     3481 gccatatctg tcgctccttc cagaagtcag atgaccttcg cttcgttctt tagcttatcg
     3541 accaactccg ccacatcctt gactttgacg cccgctttgc ggccttgagg aggttcgaac
     3601 ttcttgtaga tgacacgcag cgaggtatcc gccacgagat cgccgagttt caacacatcg
     3661 aggggctttt tcttggcctt catgatcgcg ggcagaggcg caaaacgaac cccatcattg
     3721 tatttgaaat cccgcttggt ttgcgacgag tacacgctgg tcggcgcggt gatgcgaaga
     3781 tcgactgtca caagccccgg agctttcacc ttcaggttga tgacaccacc gtccacttcg
     3841 cgccccacat gcaaggcgcc ggcctcttcc acgatggtcg ctgccgcggt agccatcggc
     3901 caaccgaggt attccgcaag gaattgaccc acctggttgg tatcgccatc caccgcttgc
     3961 ttgcccatga gcacaaaatc gggtttctcc ttgtccacga tggctttcag agccaaagca
     4021 accacgttgc cgtcgagttg atcgtcatcg gcctcgattc gaatcgcccg gtcggcacca
     4081 gtagccagca tggcgcggag cgtggtttcg cattccttgg gccccagggt gacgacaacc
     4141 acttctcccg cacgaacctt gttctgtgca ccgttttcag taaggcgaag ggcggcttcg
     4201 actgcgtatt cgtcgaacg
//
LOCUS       DAOSOR010000519         4211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_21129_length_4211_cov_4.085900, whole genome shotgun sequence.
ACCESSION   DAOSOR010000519 DAOSOR010000000
VERSION     DAOSOR010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21129_length_4211_cov_4.085900"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="PK710_20820"
     CDS             complement(<1..773)
                     /locus_tag="PK710_20820"
                     /inference="COORDINATES: protein motif:HMM:NF023324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3416 domain-containing protein"
                     /protein_id="HOT12233.1"
                     /translation="MFDDPHRTIAIERPSPEIDDGRFPIKREVGDVVQVEVDIFKEGH
                     ERLRAEVLVRPVGAPEWSAYPLELFDNDRWRAAFPVTSLGRWEYTFEAWVDRYGSWLD
                     EVKKKIGAGEDIASELLEGEQLMRGAAERASAGDKKHIEAFLASFHSLRSAVERVEAA
                     SEGAWVKLMAKLEDRSRATRYDRVLEIVVDPVHARFSAWYEIFPRSQGTDPTRGATFR
                     EAALRIPEIRAMGFDVLYMPPIHPIGKTNRKGKNNARVG"
     gene            complement(801..2981)
                     /gene="glgB"
                     /locus_tag="PK710_20825"
     CDS             complement(801..2981)
                     /gene="glgB"
                     /locus_tag="PK710_20825"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOT12234.1"
                     /translation="MSIHRNEIVAIADAQHGQPFQVLGPHQDEHGACIRAYHPMAVAC
                     WIVGIDDGKLLAEAKLIEPRGVFEAALTSIPSAYRLRYRNEKGDEWEEEDPYRFASQL
                     SEFDLHLLREGTHYRTWEKLGAHLVTIDGVHGVRFVVWAPNAKRVSVVGDFNQWDGRR
                     HAMRKHEGNGLWEIFVPGVGHGDVYKFEVFPNHGELAMLKADPFAFYTEVPPKSASRV
                     YDLSGYSWKDDAWMAARAKAEPLAEPVSVYEVHLGSWMHKEDGFSLSYREAAHKLAAY
                     VKEMGFTHVELLPITEHPFYGSWGYQTMHFFAPTSRFGTPSELMEFIDVLHENGIGVI
                     LDWVPAHFPTDGHGLAEFDGTHLYEHADPREGIHRDWNTMVFNYGRTEVWNFLLASAL
                     FWMEVYHFDGIRVDAVASMLYRDYSRKEGDWIPNRFGGRENLEAVEFIKHLNVLLYQY
                     FPGVITIAEESTAWAGVSRPTYLGGLGFTFKWNMGWMHDVLGYMSKEPIHRKYHANEF
                     TFSMLYAYTENFVLALSHDEVVYGKGSLLRKMPGDDWQKFANNRLLFGYMFTHPGKKH
                     LFMGMEFGQWNEWDHNRGLDWHLLSYAPHQGLQRWLKDLHALYRSEPALTQLDYAPEG
                     FRWIDCHDTDNSVYSYLRYAKDQSRYVVVVCNFTPVPREGYRIGVPEPGWYAERLNSD
                     SGIYGGSNMGNAGGLQAEPTPWQGFDWSISLTVPPLACLILQRS"
     gene            3312..3791
                     /locus_tag="PK710_20830"
     CDS             3312..3791
                     /locus_tag="PK710_20830"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT12235.1"
                     /translation="MNRPFRVLGVQQIAVGSRSKENLRRLWVDTLGLSLQGSYRSEKE
                     NVDEDIAVLGTGPWRVEVDLMEPIDPDKAPRVHEPALNHVGLWVDDLARAVAWLTEQG
                     VRFTPGGIRKGAAGHDVCFIHPKGNDAFPLGGEGVLIELIQAPPTVIEAYDRDAKHS"
     gene            3957..>4211
                     /locus_tag="PK710_20835"
     CDS             3957..>4211
                     /locus_tag="PK710_20835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12236.1"
                     /translation="MRQRLQPPRTRFPTRSASHRQRQALARLLVSVAVASTAIFAQEP
                     IFAAPTAAEIKEARALFQAGLALEAAGDYKNALTKFHEVAA"
BASE COUNT          994 a         1276 c         1058 g          883 t
ORIGIN      
        1 aaccccaccc gggcgttgtt cttgccctta cgattcgttt ttccaatcgg gtgaatcggc
       61 ggcatgtaca acacatcgaa gcccatcgcg cggatttcgg ggatacgaag tgctgcctct
      121 cgaaacgtgg ccccccgcgt cggatcggtc ccctgcgacc ggggaaaaat ctcgtaccat
      181 gcgctgaatc gtgcgtgtac ggggtcgacg acaatctcca agactcgatc gtagcgggtc
      241 gcacggctac ggtcctcgag ctttgccatg agtttgaccc aggctccttc ggaagcggct
      301 tccacccgtt ccacggcact tcgcaggctg tgaaaagaag ccaaaaacgc ttcgatatgc
      361 tttttgtccc cggctgacgc gcgttcggcg gcgcctcgca tgagttgttc tccctccaac
      421 aactcgctag cgatgtcttc ccccgcgccg atcttctttt tcacttcatc gagccaggaa
      481 ccataccggt ctacccaagc ttcgaaggta tattcccaac gtcccaagga agtgacggga
      541 aacgcggccc tccaacgatc gttgtcgaag agttccaagg gataggcgga ccattccggc
      601 gctcccacag gccgaaccag aacctcggcg cgcaatcgtt catgaccttc cttgaaaata
      661 tccacttcca cctggacgac gtcccccact tcccgtttga tcgggaagcg accgtcgtcg
      721 atttcgggcg aagggcgctc gatggctatg gtgcgatggg gatcgtcgaa cacgtcattc
      781 tctccttgtc gaagccagac tcaggaccgc tgcaaaatca aacaagccaa cgggggaacc
      841 gtcaggctga tggaccaatc aaatccctgc caaggggtcg gttcggcttg cagcccaccg
      901 gcgtttccca tgttgctccc cccgtatatc cccgaatcgc tgttgagtcg ctccgcgtac
      961 caccccggct caggcacccc gatgcggtat ccttcccgcg gcacgggggt aaaattgcaa
     1021 acgacaacga catatcggga ttggtctttt gcgtagcgga gataagaata tacggaatta
     1081 tcggtatcat ggcaatcgat ccagcggaat ccttccggag cataatcgag ttgcgtaaga
     1141 gccggttccg agcgataaag cgcgtgcaaa tccttgagcc aacgctgtaa tccttgatgg
     1201 ggagcgtacg acagaagatg ccaatcgagg cctcggttgt ggtcccattc gttccactgg
     1261 ccgaattcca tacccatgaa caagtgtttt tttcctggat gggtaaacat atacccaaac
     1321 aacaagcggt tattggcaaa tttttgccaa tcgtcgcctg gcattttccg aagcaatgac
     1381 cctttaccgt ataccacctc gtcgtgcgaa agcgcgagta cgaagttttc cgtataggca
     1441 tataacatgc tgaaggtaaa ttcattcgcg tgatatttac ggtggatggg ttccttggac
     1501 atgtagccaa gcacatcgtg catccagccc atgttccact tgaacgtaaa accaagtccc
     1561 cccaggtagg taggacgaga aacgcccgcc caggcggtgg attcctccgc aatggtgatc
     1621 accccgggaa agtactggta caacaacaca ttgagatgct tgatgaattc gaccgcctcc
     1681 aggttctctc tgcccccaaa acggttcgga atccaatctc cttcttttcg agaataatcc
     1741 cggtacagca tcgatgccac cgcatcgacc cgaatgccat cgaagtgata cacttccatc
     1801 caaaatagcg cgctcgccaa caagaagttc cagacttccg tccgcccata attgaatacc
     1861 atcgtgttcc aatcccgatg gatcccttcc cgggggtccg catgctcata gagatgcgtg
     1921 ccatcgaatt ccgccaagcc atggccatcc gtagggaagt gggcaggaac ccagtccaga
     1981 atcactccga ttccattttc atgaagaaca tcaataaatt ccatcagttc cgacggagtc
     2041 ccaaagcggc tcgtcggcgc aaaaaaatgc atggtctggt atccccacga gccataaaaa
     2101 ggatgctcgg taatgggcaa taactcgaca tgcgtgaacc ccatctcttt gacataggcg
     2161 gcaagtttgt gcgccgcctc ccgataggac aacgaaaaac cgtcttcttt atgcatccaa
     2221 gagccgagat gcacttcgta taccgagacg ggctccgcca ggggctcggc cttcgcacgc
     2281 gccgccatcc atgcatcgtc tttccatgaa taccccgata gatcatacac gcgggaagcg
     2341 gacttgggag gcacttccgt ataaaaggcg aaaggatccg ccttgagcat cgccagctca
     2401 ccatgattgg gaaatacctc gaatttatag acgtccccat gccctacccc aggcacgaaa
     2461 atctcccata agccattgcc ctcgtgtttc ctcatcgcgt ggcgacgacc atcccattgg
     2521 ttgaaatcac cgaccacact cactcgtttc gcattgggcg cccacacgac gaaccgaacg
     2581 ccatggacgc catcgatggt cacgagatga gccccgagtt tttcccatgt ccgataatgc
     2641 gttccttcac gcagcaggtg caaatcaaac tcactgagtt gagaggcaaa gcgatacgga
     2701 tcctcttcct cccattcgtc ccctttttcg tttcggtacc ggagccggta ggccgaagga
     2761 atgctggtca acgcagcttc aaaaacacct cttggctcga tgagtttcgc ctccgccagc
     2821 agcttgccgt catcgattcc cacgatccaa caagccacgg ccatgggatg ataggcgcga
     2881 atacatgccc catgttcgtc ttgatgcggt cccaacactt gaaaaggctg tccatgctgg
     2941 gcgtcggcga tggcaacgat ttcgtttcga tggatggaca tgctcgaatt ctccttcagg
     3001 ggctgcgcaa tcgcgcgact atatgagggg aaacccgcac gaagtcaacc aagcatgcca
     3061 acttcccaac gcttcccgca accgcaacaa gtgccggaac caggacggtt cccacgaatc
     3121 attttgatgc cgcaagcacc agaattcacg atctggctct ccttacgtga accgtcttct
     3181 tggcttgttt gctcgaccgg cccgacctag gctacgacga acaccttgtg cggactcgtg
     3241 ccgcattcgt cccgcaaaac aagggttccc ataagccacg gcgtgcgtgc aacacgtgag
     3301 gagacaagaa tatgaatcga ccgttccggg tgctcggcgt gcaacagatt gccgtaggaa
     3361 gcagaagcaa agaaaacctt cgtcgcttgt gggtggacac gctcggcttg tccttgcaag
     3421 gcagctaccg aagcgaaaaa gaaaatgtcg atgaggatat tgccgtgctc ggaaccggcc
     3481 cgtggcgcgt ggaagtcgac ctgatggagc ccatcgatcc cgacaaagca cctcgcgtgc
     3541 atgagcccgc cctcaatcac gtgggattgt gggtcgatga cctggctcgc gcagtggctt
     3601 ggctcacgga acaaggcgtg cggttcaccc caggaggaat ccgaaaagga gcagcaggac
     3661 atgatgtctg cttcatccat ccgaaaggca atgatgcctt cccgctcgga ggtgaaggcg
     3721 tactcatcga actcatccaa gccccaccga ccgtcatcga agcttatgac cgggatgcaa
     3781 aacactcctg aacccagagg ttcgcaatat ttatcataaa atacgcaagc ttaacctttc
     3841 cccgccaagc ggctccgcgt tgttcggccc taggacgaat gcgcaaccat cccttccaac
     3901 gcttgtccgc cagcgatgag gaaagtacgc tctggtaggg aaggatagcc tcgtccatgc
     3961 gtcaacgact tcaacccccg cggacccgtt ttccaacacg ttccgcaagc catcgccagc
     4021 gacaagcact cgctcgtttg ctcgtgtcgg tagcggtcgc aagcacagcg atcttcgcgc
     4081 aagagccgat cttcgccgct ccaaccgccg ccgaaatcaa agaagcacga gctttgttcc
     4141 aagccgggtt ggccttggaa gccgcgggcg attacaaaaa cgctctaacc aagttccatg
     4201 aagtcgccgc g
//
LOCUS       DAOSOR010000520         4191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_21281_length_4191_cov_3.767650, whole genome shotgun sequence.
ACCESSION   DAOSOR010000520 DAOSOR010000000
VERSION     DAOSOR010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21281_length_4191_cov_3.767650"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4089
                     /locus_tag="PK710_20840"
     CDS             <1..4089
                     /locus_tag="PK710_20840"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HOT12237.1"
                     /translation="TVRDGGRACQYGGTHTFDAITLINGAVVYVAPFNGSDKNATGNL
                     VFKSKSTIRIDATSRITAKGAGYQKKLCDNGSGPTATAGGRGGCAVKDSGGGGAHRGR
                     GGQGTKDCFVYGNSQTCQFPQEWEENCGYLAPSGTSCVYADGLNGAHNCSNPNYTCAN
                     CDGDPSVAGEPYYHHILQPEFGASGGDKGCADGWDGSVRSGSGGGRIVLFAANDNQSG
                     ELILEGRVVAHGERGCASGNDSAGGGAGGTILLVGDHVDIRATARILAHGGRGGDSQP
                     KCLPCTTNANCESGQTCVQGRCSPCNCTPCQPPSNTCPAGYPCKNLGGALGNVCVNAA
                     GTCDPYDPGENEAECAGTQNSGTCDDCAGGGGGGIINVQSRVASIDPKAFFDVRGGQG
                     GICPICAGEAGAGSGELQLDGAYLGEVCDGFDNDFNGLIDDGLGELKCAGGKTIPACI
                     NGIPQQCPADHPACVLPVTDTRPRFVVVLDTSGSMLTDIRGVPTFGDGSPNHSGVDTA
                     SDPDLIDGNNSRLFTAKRALTQVLSAFPHADYALARYHQDVGLNRSCQTASWFECQNN
                     CCSYDDPRNNVPPAFEWIPACVLPTIYPGAGYPSALSSNINIGWTSQNDCINYAGSCG
                     IPRRGADILVGFDAPLEQHLMWLDGRETAFNPSTVPADHCAFGAGGDCEIRATGPTPL
                     AGSLHAVHDYLTPIVACDGAIPCRKYSVILLTDGAESCQGNPVAAAQNLRTLDPRTSV
                     DTFVVGFSVLPEEESQLNAIAHAGGTNKAFLVGSESALANALASIIAASSVYELCNDL
                     DDDCDGLIDEDFPEKGMACHDSGLGVCQGTGIRVCNATKDGTVCMITNPGASPTPEIC
                     NGLDDDCDGLIDEDGVCDTCIPMSEVCNGKDDNCNSIVDDDPIDVGVACGYSLGECVP
                     GKTKCVNGVLICDGEVGPHPEVCDGLDNDCDGVVDGMTRLCYTGAAGTQGVGICRAGS
                     EACIAVQGSGVEAWGACIGQITPASEICDGLDNDCNGLVDDRVPDGLGHFTGDPCCAH
                     GSKCGTGECTEGVYVCAGSQVVCDGGKGPSMELCDHLDNDCNGAIDDVPGIGTACILP
                     SGCAGELACDYATGGLACVSPTIPVLEICNGIDDDCDGQVDEEPEVAQNDSRVGVACK
                     VPEPPHDKPPCKAGITVCRNGVIVCEGAIEPEPEICDGIDNDCDGIPDQPDPCPGDTV
                     CDQGICVAPCGSGEFPCPGGFSCVDGYCVPNSSLDAGTGGSGGTAGAGGSGGSSVDAG
                     DDGALADGAIHDSSMGGSGGTTGMGGMAGNNPDASAAGTGGKGSGPPPENYGLTTGGG
                     GIACGIAVPKTNRRWLMLGALGLVALAVRRSRREAFGQGGWR"
BASE COUNT          860 a         1027 c         1389 g          915 t
ORIGIN      
        1 acggtgcgtg atggcggcag ggcatgccag tatgggggaa cgcatacgtt cgatgcgatc
       61 acgctcatca acggcgctgt cgtttacgtt gcgccattca acggttcgga caaaaacgcg
      121 acgggaaatc tggtattcaa gagcaagagc acgattcgca tcgatgcgac ttctcgaatc
      181 acggcgaagg gggcgggata tcagaagaag ctatgtgaca acggcagcgg gcccacggcg
      241 acagccggag gaagaggcgg gtgtgcggtc aaggattctg gtggtggggg agcgcatagg
      301 ggacgagggg ggcagggaac caaggattgt ttcgtgtatg gcaacagcca gacctgtcaa
      361 tttccgcaag aatgggagga aaattgcggc tatttggcac cttcgggaac cagttgcgtg
      421 tacgccgatg ggttgaacgg agcgcataat tgttccaatc ccaactacac ctgcgcaaat
      481 tgcgacggcg atccatcggt tgccggcgag ccttactatc accatatctt gcagcctgaa
      541 ttcggggcat ccgggggtga taagggatgc gcggacggtt gggatggcag tgttcgctcg
      601 ggaagtggcg ggggccggat cgtgcttttt gccgcaaacg acaatcaatc cggcgaatta
      661 atcctcgagg ggcgtgtggt ggctcatggt gagcggggct gtgcttcggg caatgattcc
      721 gcgggtggcg gcgcgggagg aaccatcttg ctcgtgggag atcatgtgga catccgagct
      781 accgcgcgaa ttcttgccca tgggggaaga ggcggagatt ctcaacccaa atgtctgccg
      841 tgtacgacga atgcgaattg cgagtcgggg cagacttgtg tgcaaggacg ctgttcgccc
      901 tgcaattgca cgccctgtca gccgccgtcc aacacttgtc cagccggata tccgtgcaaa
      961 aacctcggtg gagcgttggg caatgtatgt gtcaatgcgg cgggcacttg tgatccctat
     1021 gatcctgggg aaaatgaggc agagtgcgcg gggacgcaaa actctggaac gtgcgatgat
     1081 tgcgccggcg gaggaggcgg aggaatcatc aacgtgcaat cccgtgttgc ctccatcgat
     1141 cccaaggcat tctttgacgt tcgaggaggg caagggggaa tctgccccat ttgcgcaggg
     1201 gaagcggggg ctggatcggg ggaattgcag ctcgatggtg cctatcttgg ggaggtctgc
     1261 gacggtttcg acaatgactt caatggtctc atcgatgacg gtcttgggga gctgaaatgc
     1321 gcggggggaa agaccattcc ggcatgtatc aatggcattc cacagcaatg tcccgctgac
     1381 cacccagcgt gtgtgcttcc cgtcacggac acgcgacctc gtttcgtggt ggtgctggat
     1441 acatcgggct cgatgctgac cgatatccgc ggtgttccca cctttggcga tggttcccca
     1501 aaccattcgg gggtggatac cgcgagtgat ccggacctca tcgacggcaa caattcgcgt
     1561 ctgttcacgg ccaagcgagc gctgacgcaa gtcttgtctg cctttcctca cgccgattat
     1621 gcgttggctc gctatcacca ggacgttggt ttgaatcgta gttgtcagac agcttcatgg
     1681 ttcgaatgtc aaaacaactg ctgtagttat gacgatccga gaaataatgt cccgccggct
     1741 ttcgagtgga ttcccgcttg cgtgctgccc acgatctatc ctggggccgg gtatccatcg
     1801 gcgctttcct ccaacatcaa catcggttgg acgagtcaaa atgactgcat caactacgcc
     1861 ggttcctgcg gcatacctcg tcgcggtgcc gatattctcg tggggttcga tgcgccgttg
     1921 gagcagcact tgatgtggct cgacggtcgg gaaacggctt tcaatcccag tacggtaccg
     1981 gcagatcatt gtgctttcgg tgccggagga gactgcgaga ttcgcgccac cggacccacg
     2041 cctctcgcgg gttccctgca tgcggtgcat gattacctca ctcccatcgt ggcctgtgat
     2101 ggcgcgattc cttgcagaaa atactcggtc attcttctga cggatggcgc cgagagctgc
     2161 cagggaaacc cggtcgccgc tgcccaaaac ttgcgaactc tcgaccccag aacgtctgtc
     2221 gatacgttcg tcgtgggctt cagcgtgttg cctgaagaag agagccaatt gaatgccatt
     2281 gcccatgccg gcggcaccaa caaagccttc ctcgtgggaa gcgaaagcgc tcttgccaat
     2341 gcgcttgcct ccatcatcgc tgcttcttcc gtctatgagc tgtgcaatga cctcgatgac
     2401 gattgtgacg gtttgatcga tgaggacttt cctgaaaaag gaatggcctg tcacgacagc
     2461 ggtttaggag tttgccaagg aacgggaatc cgcgtttgca atgccacgaa agacggaacc
     2521 gtatgtatga tcacgaatcc gggagcaagt ccgactcccg aaatatgcaa cgggctcgac
     2581 gatgattgtg atggcctcat cgacgaggac ggggtttgtg acacgtgtat tccgatgtcg
     2641 gaagtgtgca atggcaagga tgacaattgc aacagcatcg tggatgacga tccgatcgat
     2701 gtgggggtag cgtgtgggta ttcgttgggg gagtgcgtgc ctggcaaaac gaaatgcgtc
     2761 aatggcgtac tcatctgtga tggggaagtg gggccgcatc cggaggtctg tgatgggctc
     2821 gacaacgatt gcgatggggt ggtcgatggg atgacgcgcc tatgttacac gggtgcggcg
     2881 ggaacgcagg gcgttgggat ctgtcgcgcg ggctccgaag cttgtatcgc ggtgcaaggc
     2941 agtggtgtcg aagcctgggg agcatgcatc ggacagatca caccggcttc ggagatctgt
     3001 gatgggctcg acaacgattg caacggtttg gttgacgacc gcgtgcccga tggtttgggt
     3061 cacttcactg gagacccctg ctgtgctcat ggttccaaat gtggcacggg tgaatgcacc
     3121 gaaggggtct acgtctgcgc gggttcgcag gtggtttgcg atggtggaaa gggaccatcg
     3181 atggaactgt gcgaccatct cgacaacgat tgcaatgggg cgatcgatga cgtgcctggc
     3241 atcggaaccg cgtgtatttt gcccagtgga tgtgcgggag agcttgcgtg tgattacgca
     3301 acgggtgggc ttgcttgcgt gtcgcctacc attccggtgt tggagatatg caacggaatc
     3361 gacgacgatt gtgatgggca agtggatgaa gagcccgagg tagcgcaaaa cgattctcgc
     3421 gtgggggtag cgtgcaaggt accggagcct cctcatgaca agccgccgtg caaagcaggt
     3481 atcacggtat gccgcaacgg cgtcattgtg tgcgagggag ctatcgaacc agagccggaa
     3541 atctgtgatg ggatagataa cgattgcgat ggaattcctg atcaacccga tccttgtcca
     3601 ggggatacgg tatgcgatca aggaatatgc gtggctcctt gtggttccgg ggagttcccc
     3661 tgtccaggcg ggttttcttg tgtcgatggc tattgtgttc ccaattcatc gttggacgcg
     3721 ggaacggggg gctctggagg gaccgcggga gcgggaggtt ctgggggaag ttcggtggat
     3781 gcaggagatg atggcgcctt ggccgatggc gccatccatg attcgagtat gggaggcagc
     3841 ggtggaacca cgggtatggg tggtatggca ggaaacaatc cggatgcttc tgccgcgggt
     3901 acgggaggga aggggtctgg gcccccaccc gaaaactacg gacttaccac cggcggcgga
     3961 ggaattgcct gcggaattgc tgttccgaag acgaatcgtc gatggcttat gctcggtgcc
     4021 ttgggcttgg ttgccctggc cgtgcgccgt tcacgacgag aggctttcgg tcaaggaggg
     4081 tggcgatgag ccttgatttg cgcatgggtg aggccgcctt actcgccgtc ttggttggtt
     4141 tgaatactgg ttgttcaaac gaagagtaat gaaacaacag cggtgatcaa g
//
LOCUS       DAOSOR010000521         4190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_21293_length_4190_cov_3.626844, whole genome shotgun sequence.
ACCESSION   DAOSOR010000521 DAOSOR010000000
VERSION     DAOSOR010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21293_length_4190_cov_3.626844"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PK710_20845"
     CDS             <1..386
                     /locus_tag="PK710_20845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12238.1"
                     /translation="NEEDIEEEPSSPTTPQPRHAAPLDQEEHAVFVVGADDDFIRQCH
                     RAFYLLPVRMVSGALMSAEARAKEVNPVVIVVPADVYAFDRYAFYRLAFEAGCPLLVW
                     EREFGPEQVSVLVEATLRKAEQQQG"
     gene            457..1674
                     /locus_tag="PK710_20850"
     CDS             457..1674
                     /locus_tag="PK710_20850"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT12239.1"
                     /translation="MRFDPERALVEIKREFGEHGGVCPSIERSSTFTVLEPGTMPEIF
                     GGLRGPDKGGCFLYSRHFNPTVDVLARSLAAMEGTEYAVCTASGISAISCTLLQLCTH
                     GDHIVASDTVYGGTHALLAETFPQMGIQTTLVDPTNVSAFEKAITPATKVLYTEVVGN
                     PTLKVADIRALANLAHDRGIRLVVDNTFTPMVISPAQLGADVVVYSLTKFVNGASDMI
                     AGAVCATKDFIFQLMDLHTGRVMLLGPTMDPRTAFDIYQRLPHLALRMREHSRRALAI
                     AQRLESIGAPVVYPGLESHPQHALMRSMGNEGFGFGGLLTLDCGTVQRAEKTMSLLQN
                     EEGFGYIAVSLGYFDTLISCSGSSTSSEIPPEDRERIGLSPGLLRIAVGYSGTLEDRM
                     AQIERAVRKAELV"
     gene            complement(1772..2482)
                     /locus_tag="PK710_20855"
     CDS             complement(1772..2482)
                     /locus_tag="PK710_20855"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOT12240.1"
                     /translation="MMQSNTNPITLALDVGGSGVKGRCFRQPDEPVTERVRIKTPRPA
                     KPEAVIATIIKLLEKQRPFDRVSIGFPGVVIEGVTQNAPNLDGQWKGIDLRHRIEQVA
                     KAPVRVVNDADMHGYGAIEGRGVEMMITLGTGMGAALFSDGRLLPNLELGHHPFEKGF
                     TYEERLGQAALDKVGKETWNHRAQRAFALLQRVFNYRVLYVGGGNVRKLTGKQPDTIV
                     LVPNAIAFSGGVKLWDAR"
     gene            complement(2515..4065)
                     /gene="glgA"
                     /locus_tag="PK710_20860"
     CDS             complement(2515..4065)
                     /gene="glgA"
                     /locus_tag="PK710_20860"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HOT12241.1"
                     /translation="MLPDAFDARTSLPRPLRVLIISAELAPFARAGGLGDVMQGLSHA
                     LGEMGVEVVVATPLYGVTRLDSAVAPQRWKRPIWIPIGGDVRQVGVIETVTDGDVRVL
                     LLDCPPLFKRDSIYGTAQEPYPDNDLRFATLGMAALRAADLMFHGPPDIIHAHDWHAA
                     LAIAYARLSENEAWREAASVFTFHNIAYQGSFPITRAPMLGLHAPILHEDAVVANGSI
                     NYLKTGIVYSDRVTTVSQTYAQEVLEPEHDHGLTEVLAARSDSLVGITNGIDTTLWDP
                     SRDRALAARYDAHDPTGRIACRTALAAELGLDMSHDPPIVGVVSRLTHQKGIDMLLQV
                     VPTLVDDGMRLAIVAVGDQQWQSKLEGLSEQYRKKVAFRCAFGDPIARRVYAGADLFL
                     MPSRFEPCGLGQQYAMRYGSVPVVNPVGGLADTVEPFDETHATGTGFWLEEPTPNGIV
                     AALRRAVAIRKNANLWNQMVRNGMTRNASWDASARSYYALYRELVGADPRPSLPPPVP
                     VGASLARS"
BASE COUNT          873 a         1174 c         1215 g          928 t
ORIGIN      
        1 tgaacgaaga agatatcgag gaggagccat cgtcgccaac gaccccgcaa ccacgacatg
       61 ctgcgccatt ggaccaagag gaacacgccg ttttcgtcgt aggtgccgat gacgacttca
      121 ttcgccaatg ccaccgcgcg ttttatctcc ttccggtacg catggtctct ggcgctctca
      181 tgtccgctga agcacgagca aaagaagtca atccggtcgt aatcgtcgtt cccgcagatg
      241 tgtacgcgtt cgatcgttac gccttttatc gtctagcttt cgaggcaggt tgcccgcttc
      301 tcgtgtggga acgcgagttt gggccagaac aagtctcggt gctcgtcgaa gcgacgcttc
      361 gcaaggcaga gcagcagcaa ggctgagtgc gcgtcactat cctttacttc ccgtgcgtgc
      421 cttggtagca aactcacgtt gtcaaggagg caagtcatgc gattcgatcc ggagcgcgct
      481 ctcgttgaaa tcaagcgtga gtttggtgaa cacggagggg tttgcccttc catcgaacgt
      541 tcctccacat tcaccgtgtt ggaaccagga acgatgcccg agatcttcgg tggtcttcga
      601 ggccctgaca aaggtgggtg ctttctgtat agccgtcact tcaatccgac ggtggatgtg
      661 cttgctcgct cgcttgccgc catggaaggc accgaatatg ccgtttgcac ggcgagcggg
      721 atctcggcca tctcatgcac gctcttacaa ctctgcacgc acggcgatca tatcgtcgca
      781 agcgatacgg tctacggtgg tacgcatgcg ctgctcgccg agacattccc tcaaatggga
      841 atccaaacga cgctggtcga tcccacgaat gtgagcgctt tcgaaaaggc gattacgccc
      901 gccacgaaag tgctttacac ggaggtcgtt ggaaatccga cactcaaagt tgccgatatc
      961 cgcgcgcttg ccaatctcgc ccacgatcga ggtattcgcc tcgtcgtgga caataccttc
     1021 acccccatgg tcatatcgcc cgcacaacta ggtgcggatg tcgtggtcta ctcgttgacc
     1081 aaattcgtca atggcgccag cgacatgatc gcgggtgccg tgtgtgcaac caaagatttc
     1141 atctttcaac tcatggactt gcatacgggt cgagtcatgt tgctcggtcc gacgatggat
     1201 cctcggaccg ccttcgatat ctatcaacgc ctccctcacc tcgccttgcg gatgcgagag
     1261 catagccgtc gcgcgttggc cattgcccaa cgtctcgaat ccatcggcgc gcctgtcgtc
     1321 tatccgggcc tcgaatccca tccccagcat gcgctcatgc gttcgatggg caatgaagga
     1381 tttggttttg ggggtctgct gacattggat tgtggaacgg tacagcgcgc ggaaaagaca
     1441 atgtcgcttc ttcagaatga ggaaggtttt ggttatatcg cggtttctct aggctacttc
     1501 gatacgctga tctcctgctc gggctcgtcg acttcctctg agattcctcc ggaagatcgg
     1561 gagcgaatcg gattgtcgcc aggtttgctt cgcattgcgg tggggtattc tggcaccctc
     1621 gaagaccgca tggcgcaaat cgaacgagcg gttcgcaaag cagagttggt ttgacgtatc
     1681 gatccacgtg aatgttccgg cagcgatttc gatgagaagg tcgggtcgaa gacggtgaaa
     1741 gctgccggtt ttctttcgat gattcagtct ttcatcgagc atcccatagt ttgacgcctc
     1801 cggaaaacgc gatggcgttg ggaaccaata cgatggtatc gggttgtttt cccgtgagtt
     1861 ttcgaacgtt tccaccgccc acgtacagca cacggtagtt gaacacgcgt tgaagcaagg
     1921 caaaagcccg ttgcgctcga tgattccaag tttcctttcc tactttgtcc agggcggctt
     1981 gtcccaggcg ctcttcgtac gtaaagcctt tttcgaatgg gtggtgcccc agttcgagat
     2041 tgggcaacaa gcgtccatcc gaaaaaagcg cggctcccat gccggtgccg agcgtgatca
     2101 tcatttccac accccgccct tcgatcgccc cataaccatg catgtcggcg tcgttgacca
     2161 ccctgacggg agctttcgca acctgttcga tgcgatggcg caggtcgatt cctttccact
     2221 ggccatcgag gttgggagcg ttttgggtca caccttcgat gacgactccg gggaatccta
     2281 tggatacgcg atcgaaaggt cgctgttttt ccagcaactt gatgatggtg gcgatgaccg
     2341 cttcgggttt ggcaggacgc ggtgttttga tacgaacgcg ttccgtgacc ggttcatcgg
     2401 gttgtcgaaa acatcggcct ttgacccctg aaccaccgac atcaagggcg agcgtgatcg
     2461 ggttggtatt ggactgcatc attgccagtt tattgcgtga attaccaaag ctaatcaaga
     2521 acgagccaaa gaggccccca cgggaaccgg cggcggcaac gaggggcgcg gatcggcccc
     2581 aaccaattcg cgatatagcg cgtaataact ccttgcgctg gcgtcccaag aggcatttct
     2641 cgtcatgccg tttcgaacca tttggttcca tagattggca ttttttcgga tggccacggc
     2701 gcgacgtagg gcggccacaa tgccattggg ggttggttct tcgagccaga atccggtccc
     2761 cgtcgcgtgg gtctcgtcga agggttccac ggtatccgca aggccaccca cgggattgac
     2821 gacgggcacg gagccgtagc gcatcgcata ttgctgtccg aggccgcagg gttcgaagcg
     2881 cgatggcatc aggaataggt ccgcgcccgc gtaaacgcgc cttgcgatgg gatctccaaa
     2941 agcgcatcga aacgccactt tctttcgata ctgctcggac aagccctcga gcttgctctg
     3001 ccattgctga tcgccaacag ccacgatcgc caatcgcatg ccgtcatcga cgagggtggg
     3061 gacgacttgc aacagcatat cgatgccttt ttggtgtgtg aggcgggaaa cgacccctac
     3121 gatgggtgga tcgtggctca tatcgagccc caattcggcg gccaaagctg ttctacaagc
     3181 gatgcgaccg gtcggatcgt gggcatcgta acgggcggcg agggctcgat cgcgcgaagg
     3241 atcccaaagg gtcgtatcaa tgccattggt aatgccgacg agactgtctg aacgagccgc
     3301 aagcacctcg gtgaggccat gatcgtgctc gggttcgaga acttcctgcg catacgtttg
     3361 gctgaccgtg gtcactcgat ccgagtacac aatgcctgtt ttcaagtaat tgatgcttcc
     3421 gttggccacg acggcatctt catgaagaat gggagcatgc aggccgagca tgggagcccg
     3481 cgtaatggga aagcttcctt ggtaagcgat gttgtggaac gtaaaaacgc tggccgcttc
     3541 tcgccaggcc tcgttttccg acagccgagc gtaggcgatg gcgagcgcgg catgccaatc
     3601 atgagcgtgg atgatgtcag ggggaccatg aaacatcaga tccgccgcgc gcaacgcggc
     3661 catgcctagc gtggcaaaac gaaggtcatt atccggatat ggttcctggg cagtgccgta
     3721 aatgctgtcc cgtttgaaca atggcgggca atcgagtagc aacacgcgca catcgccatc
     3781 cgttaccgtt tctatgaccc cgacctgtcg cacatcaccg ccgatgggaa tccatatcgg
     3841 acgcttccac ctttggggag cgacagcgga atcgagccgc gtcaccccgt aaagaggggt
     3901 tgcgaccacg acttccacac ccatttcacc aagggcgtgg gaaagtcctt gcatgacgtc
     3961 gcccagcccg ccagcgcgag caaagggcgc caactcggcg ctgatgatca gcacgcgaag
     4021 agggcgaggc agcgatgttc tggcgtcgaa ggcatcagga agcatgagtg tttccgatca
     4081 tgccccggct ggcgtacaga aggaagggag aagtcaatcc tacccttcgg agccagtatt
     4141 caacgcatgg cggaggtgga ggcgagaggc tggtggcgcg gtgggcctta
//
LOCUS       DAOSOR010000522         4177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_21366_length_4177_cov_4.472586, whole genome shotgun sequence.
ACCESSION   DAOSOR010000522 DAOSOR010000000
VERSION     DAOSOR010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21366_length_4177_cov_4.472586"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="PK710_20865"
     CDS             complement(<1..1174)
                     /locus_tag="PK710_20865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12242.1"
                     /translation="MIRAGCRRGRRAVLWTVVLGTTQLIGCGCGSLLDRPRDMAPADA
                     ADVGMHPSASVAASVSAPQVARSDPLQWTPVLHDPRLTAVRVAFEAQDIAQASQQMRE
                     AMQRHQPQGVELCRWSLLLGRMLMQGKDPAGAALALDRAASEPSWILRDMAGMEAAEA
                     YLAAGDVERAKQRLPTLSAESLSSERGQLLRIEIVQASGDEQQAVHLWKQRLSVHPHG
                     PRWEVGALRVARALLDSSSREAEAIEALPWLRRIMSEVPTSSFDKEARALETIALQRL
                     SPQRIRDSAWPIEMKMRRCEALVNQGRRNDALRELDELLEGMSPSQQQQQVGCLATSL
                     AAKLRSQVKKTRNAGADTYEKAVLRCAQHKQELVGVLYAGAKIQAQVGRLPQAMAWF"
     gene            complement(1171..1665)
                     /locus_tag="PK710_20870"
     CDS             complement(1171..1665)
                     /locus_tag="PK710_20870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12243.1"
                     /translation="MTLRPSYPLEPLLEQRQEVVDARASDLLQRLEAAKKADVRKQRA
                     TSERQRHAAMVAEQTRREVNRFERGELVALDLMRWQAWRLAQAHHAQELAQQETIASE
                     RAVEAERREREARAQLAVARADAKVVEVHKRRFEAERRRVEAHAAEMEAEDQVTACCA
                     RRRA"
     gene            complement(1848..2432)
                     /locus_tag="PK710_20875"
     CDS             complement(1848..2432)
                     /locus_tag="PK710_20875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12244.1"
                     /translation="MPLEYQAQCLSCGHRGPVNPWGYQALVVDDARLVPLPHPYEART
                     LREQGTSFFMAGVEGRYARVDYRVCLDCGALAQHARLSFPVTLVGCLLVGLSLGLLWM
                     AGALTRLPAWVMPMLGIGSWLGVTWVAAKLVRVLYRSRQRQLPLAVQCPHCGGTRLRD
                     ISSAIRQSLPCPKCNERSFQIFPVEMLPESIAPK"
     gene            2594..3010
                     /locus_tag="PK710_20880"
     CDS             2594..3010
                     /locus_tag="PK710_20880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOT12245.1"
                     /translation="MHFATFHKKTTSDTDIIDITQDVRAVVASSKAKEGLVTVGVAGS
                     TASITTIEYESGAVSDLCAAIERLAPRQMAYEHDARWGDGNGYSHVRAALLGPCVSIP
                     LRNGALVLGTWQQLVLCDFDNRGRTREVYVHIISGS"
     gene            3207..3833
                     /locus_tag="PK710_20885"
     CDS             3207..3833
                     /locus_tag="PK710_20885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12246.1"
                     /translation="MPERQFFQPEARTKVRDAIVAIEAQTSAEVVVAVRRQSCPYRHI
                     DVGLGSLASFISLLLLLFLPWDFAVSWMPVEVLIAFGVGFALSTVYWAPKRWLVGSSK
                     LHETSWRAACTAFHENGISRTSGRNGLLVFVSMLERRVHVVTDIGIDPDGLGAAWQES
                     LAAMQQAVDRTDFESFLQALLSIGPVLGAAMPHAEDDVNELPDEPDMS"
     gene            3848..>4177
                     /locus_tag="PK710_20890"
     CDS             3848..>4177
                     /locus_tag="PK710_20890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12247.1"
                     /translation="MPTSYRRFLSTLAHRMARPGPAFFGLVAIVALLLISAIAWARPG
                     GGHGYSGPSGGSGGGGDGDGLVMIFLFLLRLCIECPVVGIPLMILFLGFLVVSTLIKM
                     RRRSYGDW"
BASE COUNT          818 a         1308 c         1175 g          876 t
ORIGIN      
        1 caaaccaagc catcgcctgg ggcaaacgcc ccacttgcgc ctgaattttg gctccggcgt
       61 acaatactcc caccagctcc tgcttgtgtt gcgcacaacg caacaccgct ttttcatacg
      121 tatccgcccc agcgttgcgg gtctttttca cctgactacg aagcttggcc gcaagggatg
      181 tcgccaagca tccgacctgc tgctgctgct gcgatgggct catcccttcg agcaattcat
      241 cgagttcccg caacgcatcg ttgcgacgcc cctgattgac aagcgcttca caacgacgca
      301 tcttcatctc gatgggccag gcagagtcgc ggattcgttg gggcgacaga cgttgcagcg
      361 cgatcgtctc cagcgcccta gcctccttgt cgaaggagct tgtgggcact tcactcatga
      421 tacgccgaag ccatggcaac gcttcgatgg cctcggcttc gcgcgaggat gaatcgagaa
      481 gagcacgggc cacgcgaagc gctcccactt cccaccgggg accgtgagga tgaacgctta
      541 gccgctgctt ccaaagatgc accgcttgct gctcatcgcc cgaggcttgc acaatctcta
      601 tccgaagcaa ttgcccacgt tcggaagaca gcgattcggc ggaaagggtt ggtagccgct
      661 gtttggctcg ttcgacatct cccgcggcca gataagcctc cgcggcctcc atccccgcca
      721 tgtcccgaag aatccacgac ggttcagaag cagcgcgatc caaggccaac gctgctccgg
      781 cgggatcttt gccctgcatc aacatcctgc ccaggagcaa ggaccatcgg caaagctcca
      841 ccccctgcgg ttggtgccgt tgcatcgcct cccgcatctg ttgcgatgct tgggcgatat
      901 cctgcgcctc gaacgccacg cgcacggccg tcagccgagg atcatgcaac accggtgtcc
      961 actgcagtgg atcgcttcgt gcaacctgcg gcgcggaaac ggaagccgcc accgaagcgc
     1021 tcggatgcat gccgacatcc gcggcatcgg cgggagccat atcccgagga cgatccagca
     1081 aactaccgca accgcaaccg atgagttgtg tcgttcccag cactactgtc caaagcaccg
     1141 cccgccggcc cctacgacat ccagcacgaa tcacgctctc ctcctcgcgc agcaggcggt
     1201 gacctgatcc tccgcttcca tctccgccgc atgagcctcg acgcgtcgcc gttccgcctc
     1261 aaaacgcctt ttatgtacct cgacgacttt ggcgtccgcg cgcgctaccg caagctgcgc
     1321 tcgcgcctca cgttcgcgac gttcagcctc gacggcacgt tcggaggcga tggtttcttg
     1381 ttgcgccaac tcttgtgcgt gatgggcctg cgccaaacgc cacgcttgcc atcgcatcag
     1441 gtccaacgct accagttcac cacgttcgaa acggttcacc tctcgacgtg tttgctcagc
     1501 caccatggcg gcgtgtcgtt gtcgttccga ggtcgcgcgt tgctttcgta catccgcttt
     1561 tttggcagct tccaaccgct gtagcaagtc gctggcccgc gcatcgacca cttcctgtcg
     1621 ctgctcaagc agcggttcga gcggatagga tggccgaagc gtcatgatgc cccccaccat
     1681 acgtgtctgt ccaagactgg caagttcgtt cccatgattc ggaaacgaag ccgcgttgga
     1741 tcaccgaatt ggagtccgaa acaaggaggc atatacctcc ggcggtccat gtctatctca
     1801 ttgatttaaa acattaataa ttgatatcgg gaccggaccg gcaaggctca tttcggggcg
     1861 atggactccg gcagcatttc aacagggaag atctgaaacg aacgctcgtt gcatttcggg
     1921 catgggaggc tctgccgaat cgcagatgaa atgtcgcgga gtcgggtccc cccacagtgc
     1981 gggcattgca ccgccaaagg gagctgacgc tgccgggagc gatacagcac gcgaacgagt
     2041 ttggcggcaa cccacgtcac cccaagccag cttccgattc cgagcatcgg catcacccac
     2101 gccggcagcc gcgtcaatgc gcctgccatc cacagcaagc ccagagaaag gcccacgagc
     2161 aaacagccca ccagcgtaac gggaaagctc aaacgcgcat gctgggccaa cgcgccgcaa
     2221 tcgagacaaa cgcggtaatc cacgcgtgca taacgccctt ccacccccgc catgaaaaac
     2281 gatgtacctt gttcccgtag ggttcgcgct tcataaggat gaggcaacgg cacgagcctc
     2341 gcgtcgtcga caaccaacgc ctgatagccc cacggattga caggccccct gtgaccgcaa
     2401 gacaaacact gcgcctggta ttccaacggc atcgactctt atcgtaacgg aagcgaagcc
     2461 gaaaagggat agaaccttgg aaccggggac acgattgaaa atgcagttgg cgtccgctat
     2521 caactcgtgc caccaaccca tgacctttcg ttgctctgtc gactcgccgc tgcttgccga
     2581 gtaggctgat gcaatgcatt tcgcaacatt tcacaagaaa acgacatcgg acaccgacat
     2641 tatcgacatc acccaggacg ttcgggccgt ggtggcctca tcgaaagcca aagagggcct
     2701 ggtcacggtc ggggtggccg gttcgacggc aagcatcacg acgatcgaat atgagtctgg
     2761 ggccgtttcc gacttgtgcg cggcgatcga acgtctggct ccccggcaga tggcttatga
     2821 gcacgatgcg cgctggggcg acggcaatgg ctattcgcat gttcgtgcgg cattgcttgg
     2881 gccttgcgta tcgattcctt tgcgaaatgg ggctctggtg ctcgggacat ggcaacaact
     2941 cgtgctttgt gactttgata accggggtag aacgcgcgag gtttatgttc acatcataag
     3001 cgggtcctga tgtcgtcgga atcgcgtgaa tcgaagggct cgcttgcgtt ttgcaccgat
     3061 atgcggcgta cgcttttgcc cgccatccgt gtttcgtcga cgcaaagcgg gtatttcgtt
     3121 cgatcgaacg tgctggacaa ggctgttggc tcgctgcagg aaggtggaat atagcggccg
     3181 cctggggcat gatcgcacca tcatccatgc ccgaacgcca gtttttccag cccgaggcgc
     3241 gaacgaaagt ccgtgacgct atcgtcgcca tcgaagccca gacgtccgct gaggtggtcg
     3301 tagccgtccg acgccaatcc tgcccatacc ggcatatcga tgtgggactt ggcagcctgg
     3361 cttcgtttat ctccttgctt ttattgcttt ttttgccttg ggacttcgcc gtttcgtgga
     3421 tgcccgtgga agtgctcatc gcttttgggg tcggttttgc cttgagcacg gtctattggg
     3481 cacccaaacg gtggctcgtc ggtagctcga agttgcatga gacatcgtgg cgcgcagcct
     3541 gcacggcgtt tcatgaaaac gggatctcac gaaccagtgg gcgaaacgga ttgctcgtct
     3601 tcgtcagcat gctcgagcgt cgggtacatg tcgtcaccga tatcgggatc gaccccgatg
     3661 ggcttggagc ggcctggcaa gaatcgctag cggcgatgca gcaggcggtg gatcgcacgg
     3721 atttcgaatc cttcctccaa gcgctcctgt cgataggccc ggtgctcggc gcggcgatgc
     3781 cccatgccga agatgacgtc aacgaactac ccgacgaacc ggatatgtca tgaacgaacc
     3841 acggtccatg ccaacgtcat atcgccggtt cttgtcgacc ctcgcgcatc gaatggcgcg
     3901 tcccggacca gcctttttcg gactggtcgc catcgttgcc cttttgctca tttccgccat
     3961 cgcttgggca cgtcccggtg gcggtcatgg ctatagcgga ccttcgggag gttccggggg
     4021 aggcggcgac ggcgacggtc tggtcatgat cttcctgttt ttgctgcgac tttgcatcga
     4081 gtgtccggtc gttggcattc cgctcatgat ccttttcctt gggtttctcg ttgtctccac
     4141 cctgatcaag atgcggcgac gctcgtatgg ggattgg
//
LOCUS       DAOSOR010000523         4176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_21372_length_4176_cov_5.289493, whole genome shotgun sequence.
ACCESSION   DAOSOR010000523 DAOSOR010000000
VERSION     DAOSOR010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21372_length_4176_cov_5.289493"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..944
                     /locus_tag="PK710_20895"
     CDS             18..944
                     /locus_tag="PK710_20895"
                     /inference="COORDINATES: protein motif:HMM:NF025055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HOT12248.1"
                     /translation="MDDETQAEFPYAKATIIYVAALSLLAAIVLLGFLIVREQHTRIT
                     HDAALINAAGRQRMLAQRMVRDVLAMEASSLAVDRTKARSSLQDTYQRWRMGHERLLQ
                     GEAGLGGDIFRVPANQEKRAEFQEKFEGFQAAIQIVVQGNGSVPENLGERCDAYVSDM
                     ESLLFEIQRDSEARSMTVSWMLQVIVGLALLLIATEGVFVFRPLIVGLRGSFGKLREA
                     HERVQHELSARMQAEQERDVLKGLLPICAGCKRIRDDEGQWQPVEMYIEERSEARFSH
                     GLCRECIRRLYPERADAILAKIDEQDVSESSH"
     gene            986..1489
                     /locus_tag="PK710_20900"
     CDS             986..1489
                     /locus_tag="PK710_20900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STN domain-containing protein"
                     /protein_id="HOT12249.1"
                     /translation="MLWLFAYRRLLGMFRSRYGAFALLWSMACGGYPEPQQPVCPASV
                     DPEWSAWNPRRTNPSKAMHVPARDTVRIESAIEDDPVDVLLRRARRRLPNLSLHQAPV
                     DETLRWFAEIGQFNVIISAEAGARKVSLSLKNVSLQAAFRAVLASARLEARLLADNII
                     EVRPKSH"
     gene            complement(1490..2008)
                     /locus_tag="PK710_20905"
     CDS             complement(1490..2008)
                     /locus_tag="PK710_20905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOT12250.1"
                     /translation="MTYDSLATVVKRIAFIAWWPLTLACDSPSSSPQPTASSRIVSSS
                     PRIEIIPFPGSTSEVRDWIRSQRERAASEGRVAVVYVGAPWCEPCQRFHDAVTQGKLD
                     ARFPTLRLLEFDRDQHEKSLQEAGCLSELIPLLAKPTEQGTCSHTRVEGGIKGDGAVG
                     FIASRLDVMMRK"
     gene            2106..2948
                     /locus_tag="PK710_20910"
     CDS             2106..2948
                     /locus_tag="PK710_20910"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT12251.1"
                     /translation="MSHDPMQDRAVVVITGASRGIGLGVACWLAVSRCPLMLTARDAT
                     ELERVATWLRSQGAVVEHVACDVADEPAMARALGAATAMGPIGGMLNNAGVLEPVGPI
                     QSLDVSAFESHMRTNVTGVLVGMRQALAHRKPEHPLRIVNVSSGAATRGYRGWAAYCA
                     SKAAVNIMTQVASLEADSMTSIVAVAPGIIETRMQRIIREMPEDRFPDVDDFRKLKQQ
                     GELLHPVDAAIALAWLLRTAPLSLSGRFLDARGDGVLRDRNGEGESAVLRAKQWFDVL
                     EAEA"
     gene            3100..3573
                     /gene="rnhA"
                     /locus_tag="PK710_20915"
     CDS             3100..3573
                     /gene="rnhA"
                     /locus_tag="PK710_20915"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF001236.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOT12252.1"
                     /translation="MSEAKQVQIFSDGSCLGNPGPGGYGVVLLWGEARKELSCGYRRT
                     TNNRMELLAAIEGLRALKRPCHVTLTSDSEYLRQGITQWIRAWKKNGWRTSSKAPVKN
                     ADLWRRLDELAERHTVQWRWVKGHAGHVENERCDTLARTAAQYHATERDMGYEQG"
     gene            3688..3966
                     /locus_tag="PK710_20920"
     CDS             3688..3966
                     /locus_tag="PK710_20920"
                     /inference="COORDINATES: protein motif:HMM:NF003817.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HOT12253.1"
                     /translation="MSERMVQCRKLGKLLPGLPKPPYRNALGQKIYEEVSKEAWDMWL
                     HESVRIINTYRVDLASKEGQQFMLEQTKVFFGYEEGDLAATAWTPPAS"
     gene            complement(3967..>4176)
                     /locus_tag="PK710_20925"
     CDS             complement(3967..>4176)
                     /locus_tag="PK710_20925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12254.1"
                     /translation="MIQTRSRVAPGGRWSHPDEFHWHLEIIPRLTQVAGFEWGTGFYI
                     NPTPPEQAAQFLRDVLIPIQLVGQS"
BASE COUNT          873 a         1006 c         1387 g          910 t
ORIGIN      
        1 acccataaat atcaatcatg gacgacgaga cgcaggcgga attcccgtat gcgaaggcga
       61 cgatcatcta cgttgccgcg ctgtcgttgc tggccgcgat cgtattgctg ggatttttga
      121 tcgtacggga gcagcacact cgcattacgc acgatgcggc gctgatcaat gcagcgggtc
      181 gccaacgtat gttggcgcaa cggatggtca gggacgtgtt ggcgatggag gcatcatcgc
      241 tggcggtcga tagaacgaag gctcgaagca gtctgcagga cacgtatcaa agatggcgaa
      301 tggggcatga gcggttgttg cagggggagg ctggcctggg cggggatatt tttcgcgttc
      361 ctgccaacca ggagaaacgc gcggagtttc aggaaaaatt cgagggcttt caggcggcga
      421 ttcagattgt tgtccagggg aatgggagcg ttccggaaaa cctcggggag cgttgtgacg
      481 cctacgtatc tgacatggag agtctgcttt tcgaaataca gcgcgacagc gaggcgcggt
      541 cgatgaccgt gtcgtggatg cttcaagtca tcgtgggctt ggcgttgcta ttgattgcga
      601 cggagggcgt gttcgtgttt cgtccactca tcgttgggtt acgcggttcc tttggaaaat
      661 tgcgtgaagc tcatgaacgg gtacagcacg agctttccgc tcgtatgcaa gcggagcaag
      721 agcgggacgt tctcaagggc ctgcttccca tctgcgctgg atgcaaacga atccgcgatg
      781 acgaaggtca atggcagccc gtggaaatgt atatcgaaga gcgttcggaa gcgcgctttt
      841 cgcatggact ctgtcgtgaa tgcattcgtc ggctgtatcc tgaacgggcg gatgccattc
      901 ttgcgaagat cgatgagcag gacgtttccg agtcttcgca ttagcttgcg gtcttcaatg
      961 acgtggactc gatggatgtc gagccttgct ctggctgttt gcgtatcgca gattgcttgg
     1021 catgtttcgt tcacggtatg gcgcgttcgc tttgttgtgg agcatggctt gcggtggcta
     1081 tccagagccg caacaacctg tttgtcctgc ctctgtcgac cccgaatggt ccgcgtggaa
     1141 tccgaggcga acgaatccgt ccaaggcgat gcatgtgcct gcgcgcgata ccgttcggat
     1201 cgagtctgcg atcgaagatg atcccgtgga tgtgttgttg cggcgcgcga ggcggcgctt
     1261 gccgaacctt tcgttacatc aagcgccggt ggatgagact ctccgatggt ttgcggaaat
     1321 cgggcagttc aacgtgatca tatccgccga ggcgggggcg cgaaaggtga gcctttcact
     1381 gaaaaacgta tcgctacaag cggcctttcg agccgtcttg gcgagtgcga ggctcgaagc
     1441 gcgtctgctg gcggacaata tcatcgaagt tcgcccaaag agtcattgac tacttgcgca
     1501 tcatgacatc gaggcgcgag gcaatgaatc ccaccgcgcc atcacctttg attccccctt
     1561 cgacgcgtgt gtgggaacag gtgccctgct cggtgggctt ggccaaaagc ggaatgagtt
     1621 cggacaaaca tcccgcctct tgcaatgact tttcatgctg gtctcgatcg aattcgagca
     1681 gacgaagggt ggggaatcgg gcatcgagtt ttccttgggt aacggcgtcg tgaaaccgtt
     1741 ggcacggttc acaccacggt gctcccacgt agacaacagc tacgcgtcct tccgacgcgg
     1801 cgcgctcgcg ctggctgcgg atccaatccc gaacctcgga ggtggagcct ggaaatggga
     1861 tgatttcgat gcgtggggat gatgagacga tccgagacga tgccgtgggc tgcggcgagc
     1921 tggaaggtga atcgcaggca agggtgagtg gccaccaggc gatgaatgcg attcgtttca
     1981 cgacggtggc gagggaatca taggtcatgg cgtgtcgtca tccatgatag tgtgacacaa
     2041 accgtgtagt cgattcacgc aacccgaggc aacgttggtg catcggagct ttcgagtcgt
     2101 tgaccatgag ccacgatcca atgcaggaca gagccgttgt ggtgatcacg ggcgcaagcc
     2161 gcgggattgg tcttggagta gcttgttggc tggccgtgtc tcgatgcccg ttgatgctga
     2221 cggcgcgcga tgcgaccgag ttggaacggg tagcgacgtg gcttcggagc caaggggcgg
     2281 tagtcgagca tgtcgcgtgt gatgtcgcgg atgagcccgc gatggcccgt gcgctcggcg
     2341 ctgcgacagc gatggggccg atcggaggca tgctcaacaa tgcaggcgtt ctcgagcccg
     2401 taggccccat tcaatctctc gatgtctcgg ctttcgaatc ccatatgcga acgaatgtga
     2461 cgggcgtgtt ggtgggtatg cgacaagcct tggcacatag aaaaccggaa caccctttac
     2521 gcatcgtcaa tgtatcttcg ggggctgcca cgcgtgggta tcgaggatgg gcggcatatt
     2581 gcgcctccaa ggccgccgtc aacatcatga cgcaagtggc atcgttggaa gccgattcga
     2641 tgacgagtat cgttgccgtg gcaccgggca tcatcgaaac gcgtatgcag cgcatcatac
     2701 gagagatgcc ggaagaccgt tttcccgacg tggacgattt tcggaaactc aagcaacagg
     2761 gagagttgtt gcatccggtc gatgccgcga tcgcgttggc gtggttgttg cgcacggcac
     2821 ctttgtcgtt gtccggacgc tttttggatg cgcggggaga tggcgttttg cgtgatagga
     2881 atggagaagg ggaaagcgct gtactgcgtg cgaagcaatg gttcgacgta ttggaagccg
     2941 aggcctgacg ggtgggggga tggacaagga gacgcattgt ccgttcaggt ataacctatt
     3001 ggatatattg attaaattgt ctagttcccg ttcttcctgg tagggtgggc gactgttctt
     3061 tccttttctc gatcgggacg tgacgctacg aatcggcaga tgagtgaggc caagcaggtt
     3121 cagatttttt ccgacggatc atgtttgggg aatcctggtc ctgggggata tggggtcgtt
     3181 ctgctttggg gggaggcgcg caaagaactg tcctgtggat atcgccgaac gaccaacaat
     3241 cggatggagc ttcttgccgc gatcgagggg cttcgtgcgt tgaagcgtcc gtgccacgta
     3301 acgttgacgt cggattctga gtatttgagg caaggtatta cgcagtggat ccgggcatgg
     3361 aaaaaaaacg gctggcggac atcgtcgaag gcaccggtga aaaatgcgga tttgtggcgg
     3421 cgactcgatg agctggcgga gcgtcacacc gtgcagtggc gatgggtgaa gggccatgcg
     3481 gggcatgtgg aaaacgagcg gtgtgatacg ttggcgcgca cggccgcgca gtaccatgcc
     3541 accgaacggg acatggggta tgaacagggc tgagttggag cgaaaggccc tttacgtggg
     3601 gtggtgttcg aaccaagttg agagccgcct cggaattggg gcaagagaca tacaaccgca
     3661 gcgtcaagga ttgacgagga ggtcgacgtg agtgaacgga tggttcaatg ccgcaaacta
     3721 ggcaaactat tgccggggct tccgaagccg ccgtaccgaa acgctctcgg acaaaagatc
     3781 tatgaagagg tttcgaaaga ggcgtgggac atgtggctgc acgagtcggt gcgcatcatc
     3841 aacacctatc gggtggatct ggcgagcaag gaagggcagc agttcatgct cgagcagacc
     3901 aaggtattct ttggatacga agaaggcgat ctagcggcaa cggcctggac gccacctgct
     3961 tcctgatcag gactgaccga ccaattggat agggataaga acgtcgcgaa ggaattgcgc
     4021 ggcctgttct gggggcgttg gattgatata aaacccggtg ccccattcga atcccgccac
     4081 ctgggtgagc cgggggatga tttccaaatg ccaatgaaat tcgtcgggat gcgaccagcg
     4141 gccgccggga gcgaccctgg agcgtgtttg gatcat
//
LOCUS       DAOSOR010000524         4167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_21424_length_4167_cov_4.580253, whole genome shotgun sequence.
ACCESSION   DAOSOR010000524 DAOSOR010000000
VERSION     DAOSOR010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21424_length_4167_cov_4.580253"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1637)
                     /locus_tag="PK710_20930"
     CDS             complement(135..1637)
                     /locus_tag="PK710_20930"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOT12255.1"
                     /translation="MASSSFAPAVIGSLLVMATGMASLSAGIWFLLPKNGYAAEGVRI
                     EGKKIPPNTTAEQYIRELAQQKMARTVALQIPNASDSPCSLEQLGVRPDIQTTMRRVM
                     QVGRQGSLAYRIAESWRARRAEINVPLSFHVDADPLLQQVIEAKQKWDYPSTPARYDF
                     HTQSALPHKNGKALDSYGALDRLYDSLRSEENAIDLPVPVAEVPPVVTANYLDHLDMS
                     QRVGHFETRFATAGGQANRAQNIATAAARLDGIVMLPGQVISFNQLVGHRTMDNGFQK
                     AWEIFKGEMVEGVGGGTCQVASTLHAAAYLGGLDIVERSPHSRPSGYIDIGLDATVVD
                     GLVDLKLRNPFLFPVVLHSVVNQGQITFEILGEQRPVRVTFRGEVISTQRYKRKVQEA
                     SWLTEGRVLRKQRGIRGFTVRKVRLIRARDGQQREEVTRDFYPPTTEIFLVPPGTDPE
                     KDLPPLPQEGGKAQDDTDRDSSERPKLEIENAPSARAPAPTPSHHVFIDR"
     gene            1977..3704
                     /locus_tag="PK710_20935"
     CDS             1977..3704
                     /locus_tag="PK710_20935"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT12256.1"
                     /translation="MKTCPRCHLRYPSDATKCFVDGETLVAMQDPRIGLVLSGRYEIE
                     RVLAEGGMATVYVARHRVVKRACAVKILSSGLGRNQVVRERFRREAKATQKLAHPNII
                     EILDHGETDDGLPYLVMEYLEGETLADVIARGALTMPQAMPIALQIARALARAHDFDV
                     IHRDLKPDNVFLCAREGTSPLAKLLDFGIARSMHDARLTSAGEVFGTPQYMAPERIST
                     IDAGPSADLYSFGVMLFEMLTGKLPFQASDVASYFSQHIKETPPTVASMGVSVPPSLE
                     ALIASLLEKTADKRPVDAHAVCKELLEISAKARIAVPPEPRVEQVFPEEEAKSLPSTT
                     TDRWAQRTLVFEQMLAKAHGGNQPPEAKELLATIQRKVRELSVLRSDAIKEQRMLSAL
                     EGKSKEGRQRFGNAVEGLGLDASKARKEARDAREQAEKASIRTDTLRKQAMALHREVI
                     LWEGRSAFEEPYSQLADAYRRLADAIDPWFAARKLDLEASKKAVELENVVTDLEFQIG
                     ELRAGLLRLETSSEKEREAIEQRSTELSQKADSLEQELLSLASRFCEPLRAFPAVSAL
                     FRQLETGSS"
     gene            complement(3701..>4167)
                     /locus_tag="PK710_20940"
     CDS             complement(3701..>4167)
                     /locus_tag="PK710_20940"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT12257.1"
                     /translation="FREDLFFRLGVVTIPVPSLRERIEDVPLLVKHFLDIHGKNRKIK
                     LTREAWKMLESHSWPGNVRELENEIRRALALSDDVIREQDLSIGVMGPQSKTTSAPSM
                     NLKTRLEELERELVREALERTAGNQSRAAQLLGVSRYGLQKMIKRLDVIHKR"
BASE COUNT          858 a         1063 c         1214 g         1032 t
ORIGIN      
        1 cgcgataacg cctactttgg tccagactgg ccgatcggat ttttggtccg gtatctcgat
       61 tacagggcgt tcagaacgtt tctgcatccc attgggccct atcacaccgc gtcaatttgg
      121 gcgaatgttt gccattatcg gtcgatgaac acgtggtgag atggagtggg ggcgggggct
      181 cgcgcggagg gggcgttttc gatttccagc ttcggtcgct cggagctgtc tctatccgtg
      241 tcgtcctgcg cttttccccc ttcttgaggc agagggggaa gatccttttc cgggtccgtg
      301 cctggaggta ccaagaatat ttctgtcgtg ggcgggtaga aatctcgggt cacttcttcc
      361 cgctgttgtc catcccttgc ccggatcaag cgcacctttc ttacagtaaa accgcgaatt
      421 cctcgttgtt ttcgcaaaac ccgcccttcc gtcagccatg aggcttcttg gactttgcgc
      481 ttgtatcgct gcgtgctgat gacttctccg cgaaaggtaa cgcgtacggg tcgttgttct
      541 cccaagattt caaaggtgat ttgtccttgg ttcacgacgc tatgcaagac cacgggaaat
      601 aaaaatgggt tgcgaagttt caagtccacg agcccatcca ccacggttgc atccaagccg
      661 atgtcgatgt agccgcttgg ccgagaatgg ggagagcgtt cgacgatatc gagtcctccc
      721 aagtaagctg ccgcgtgaag ggtgcttgcg acctggcacg tgcctccgcc gaccccttcc
      781 accatctctc ctttgaagat ctcccaagct ttttgaaaac cgttgtccat cgttcgatgg
      841 ccgaccaatt gattgaagga aatgacttgg cccggcaaca tgacgattcc atccaatcgt
      901 gcggcggccg tggcgatgtt ttgggcccgg ttggcttgtc cgcctgcggt agcaaatcgt
      961 gtttcgaagt gccccacccg ttggctcata tccaagtgat ccaaataatt tgccgtaacg
     1021 acagggggca cctccgccac gggcaccggc agatcaatcg cgttttcctc tgaacgtaac
     1081 gaatcgtaga gtcgatcgag agcgccataa gaatccagag cttttccgtt tttgtggggc
     1141 aacgccgatt gtgtgtgaaa atcgtaccgt gcgggtgtcg atgggtagtc ccatttttgt
     1201 ttggcttcga tcacctgttg caacaacggg tcagcatcca cgtggaatga taggggcacg
     1261 ttgatttcag cgcgacgtgc gcgccagctt tccgcgattc gataggcgag gcttccttga
     1321 cgaccgactt gcatgactcg tcgcatcgta gtctggatgt cgggacgcac accgagttgt
     1381 tcgagcgagc aaggtgaatc ggaggcattg gggatttgaa gggcgacggt tcgcgccatt
     1441 ttttgctgcg caagctcgcg aatatattgt tctgctgtcg tattaggagg aattttcttt
     1501 ccttcgattc gaaccccttc ggccgcatat ccgtttttgg gcaagaggaa ccagatcccc
     1561 gcggacaggc ttgccatgcc tgtggccatc acgagcaacg agccgatgac tgcgggtgcg
     1621 aacgatgagc ttgccatggt ggaaccgtga aagatcaacg ctggaaacaa catgcatcat
     1681 tccgagaatt tttgccattc ggcgtcctgg tgtgcggatc gtctgaaacg caaatccagc
     1741 aagcaagcgg gaagatattg agttttcggt aagaaccgtt gaaagatgag agcggttggt
     1801 ttggtttgta ggaacttgga atctcggcca aatggaatgg gtcggccgtg ttggatggtt
     1861 ctcaatcgat tgccttatca gacttccgtt cgcgggcgtc ctccgtttag cctaggggtc
     1921 atggtcgcgc tttcgatcat cgatgcgcgc gggttctgaa tcgtgtcagg agcagcatga
     1981 agacgtgtcc tcgttgtcat cttcggtacc cttcagatgc cacgaagtgc ttcgtcgacg
     2041 gggagactct tgtggcgatg caggaccctc gcatagggct ggtgctttcc ggacgctatg
     2101 agatcgagcg tgtgcttgcg gaggggggga tggcgaccgt gtacgtggcg cggcatcgtg
     2161 tggtgaaacg cgcctgtgcg gtgaagatcc tgagttccgg attggggcgt aaccaggtcg
     2221 ttcgcgagcg atttcgtcgg gaggcgaaag cgactcagaa gcttgcgcat cccaacatca
     2281 tcgagattct cgatcatggg gagacggacg acgggttgcc gtatttggtg atggaatatc
     2341 tcgaggggga aaccttggct gacgtcattg cgagaggggc gttgacgatg ccccaagcaa
     2401 tgcccattgc gttgcagatt gctcgcgcct tggctcgtgc gcatgacttc gatgtgattc
     2461 atcgcgatct gaagccggac aatgtatttt tatgcgcgcg cgaggggacg tctccgcttg
     2521 ccaagttgct ggatttcggc attgcgcgat ccatgcatga tgctcgttta acttcggctg
     2581 gcgaggtttt tggtactcct cagtacatgg ctcccgagcg gatttcgacg atcgatgcgg
     2641 gaccttcggc cgatctgtat tcgtttgggg tgatgctttt cgaaatgctg accggaaagc
     2701 tcccgtttca ggcttccgat gtggcttcct atttttcaca acatatcaag gagacgcctc
     2761 ccacggttgc ttcgatgggt gtatcggttc ccccatcgct cgaagcgttg atagcctctc
     2821 tgctggaaaa aacggcggac aaacgacctg tcgatgctca tgccgtatgc aaagaactgc
     2881 tggaaatctc ggcgaaagcc aggattgccg tgcctcccga accgcgcgtc gaacaagtgt
     2941 ttccggagga agaagccaag tccttacctt cgacaaccac ggatcgttgg gcgcaacgta
     3001 ctctggtctt cgaacaaatg ctcgccaaag cccatggggg aaaccaaccc ccggaggcca
     3061 aagagctgct tgccacaatt caaagaaaag tgcgtgaact cagtgtgtta cggtccgatg
     3121 cgatcaagga acagcgaatg ctctcggcct tggaaggaaa gtcgaaagaa ggacgacaac
     3181 gttttggcaa tgccgtcgaa gggttgggct tagacgcgtc caaggccaga aaagaggctc
     3241 gcgatgctcg agagcaggcg gagaaggcgt cgattcgtac cgatacgctt agaaaacaag
     3301 cgatggcttt gcatcgggag gtcattttgt gggagggccg tagcgcgttc gaggagcctt
     3361 attctcagct tgcggatgct taccggcggc ttgccgatgc gattgatcct tggtttgctg
     3421 ctcgcaagct ggacctggaa gcatcgaaga aagctgtgga attagaaaat gtcgtgaccg
     3481 atcttgaatt ccaaatcggt gaattgcgcg ctggattgct tcgccttgaa acgtcttccg
     3541 aaaaagaacg cgaagccatc gaacaacgtt cgacagaact tagtcaaaag gcagactcgt
     3601 tggagcaaga attgctcagc ctggcttcac ggttctgtga gccgttgcgg gcttttcccg
     3661 ctgtgtcggc gttgtttcgt cagctcgaaa ctggctcgtc ctaacgcttg tggatcacgt
     3721 ccagacgctt gatcattttc tgaaggccgt agcgtgatac gccaagtagt tgggcagcgc
     3781 ggctttggtt gccagccgtt cgttctagcg cttccctcac cagttcacgc tccaactcct
     3841 cgagacgcgt ttttaggttc atgctgggag ccgaggtcgt tttggactgc ggccccatca
     3901 cgccaataga caaatcttgc tctcgaatga catcatcgct cagtgctaac gctcgtcgga
     3961 tttcgttttc gagttctcgg acgtttcctg gccatgaatg ggattcgagc atcttccagg
     4021 cttcccgcgt gagtttgatt tttcgattct tgccgtggat gtcgagaaag tgtttgacca
     4081 acagcgggac atcttcgatt cgctcgcgca aagaagggac cggtatcgtg acgacaccga
     4141 gacgaaagaa tagatcttcc cgaaaag
//
LOCUS       DAOSOR010000525         4163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_21445_length_4163_cov_3.677215, whole genome shotgun sequence.
ACCESSION   DAOSOR010000525 DAOSOR010000000
VERSION     DAOSOR010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21445_length_4163_cov_3.677215"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="PK710_20945"
     CDS             <1..1078
                     /locus_tag="PK710_20945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12258.1"
                     /translation="GSTTGGSGGSGTGGTSASGGSGGSTTTEPPAGNPYPEATCTAPP
                     EAGPEDVSHPTTVVGDGTAASCTSAAVVSAIAQGGIITFDCGPAPIVITLEQTAKIFN
                     DTGPNIVIDGGGLVTLDGGGQRRILYMNTCDEAQKWTTPHCQNQDHPRLTVQNINFSN
                     GNSKNETQYDGGGAIWVRGGRFKLVNTRFLNNTCADTGPDLGGAAVRVLSQFENKPVY
                     VVNSTFGGDANRGGICSNGGAISSIGVSWTIINSLFSYNRAIGHGANPAQPGTPGGGS
                     GGAIYNDGNTMTLSLCGTRIEHNQVNAHGSAIFFVSNDHSGDIWIDRSVIKGNTGGSW
                     YPTYPQISNHSDTPIHVTDSVIEE"
     gene            1247..3670
                     /locus_tag="PK710_20950"
     CDS             1247..3670
                     /locus_tag="PK710_20950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT12259.1"
                     /translation="MRLSRLGFSWPFVALFLVAPQAAADPVEPPVRQTPAISSWPAGL
                     QAPQAVVIPLEIVVGDDGTVREVHVLSSPSEAWAEAARAEAMTWVYTPARVQGVARTA
                     RIRDQVRFEPEAQGTGQEQTPAVDATPPPNASPSLASSAPEPETSASASLPTEPIPVL
                     VTGKARPRAASDVVRNREALSAAPHRDGSELLLTVPGVFLTQHGGEGKAHQIFLRGYD
                     AVHGQDLEIWAGGAPVNDVSNVHGQGYADLHFLIPEAVNEVQASAGPFDPRQGDFAVA
                     GSVRYKLGLAEEGFLVQQGIGSHKGRRTLLAYRPEGENEETFTAFESYSTDGFGPSRA
                     AQHMSWIGQGLLPLGGHAHLRVLGSTYAGRFDSAGVIPLAWIENRGVDRFEPWIRGQS
                     GQSSRTQFVAEFFQHDDAGMEWTVAPFFILRGLGLRQNFTGWLDDPVRGDTTQQRNDA
                     TTLGATAHLKNRITVFSEDDQIEVGVVGRTDRIEQSQRAIAWGTGRETETVVDASVLA
                     NHVGGYLDASLRPIRRVTVRGGVRLDGLSYQVDDRATTGSRSAQGLRMSKRAMVDVAA
                     ATGLHLMASYGEGFRSPQARSLGEGETAPFTTVRAFESGVRFNAEERFQAQAAVFQSR
                     LSKDLVFDHATGRNETVPGTTRSGMALDLVVQPRPWLFSMASLTVVRAVFRESEGIYE
                     KGFLLPYAPQVVARSDTAYRPVVGSMAGADVRGHLGVGLTLLARRPLPYGEMGHDVFL
                     ADASARARWKAVELALEVFNLFDTAWFDGEFVYASRETRNAAASLVPQRHVTAGPPRT
                     LWLTGALHF"
     gene            3712..>4163
                     /locus_tag="PK710_20955"
     CDS             3712..>4163
                     /locus_tag="PK710_20955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12260.1"
                     /translation="MKAKSFSFSLLLGLVSTTAWLALSLGAVSLASSGCGDSAEEKTA
                     GKRVTLRTRVESADDLSKPTLNARGWSVTWSRVLVSVSSLYYYDGAPPSLARLPSPSL
                     WQRWFGISVAHAHPGHYQAGNAMGQMLTAASFDLVSGPTDLAEGEGVTG"
BASE COUNT          848 a         1280 c         1201 g          834 t
ORIGIN      
        1 cggtagcaca acggggggca gcggcggcag cggtacgggc ggaacctccg catcaggagg
       61 ctccggcggc tccaccacca ccgaaccgcc agccggtaac ccctaccccg aagcaacctg
      121 caccgcgccc ccagaagcag ggccagaaga tgtctcccat ccaacaacgg tcgtgggcga
      181 tggaaccgcg gcaagttgca cttccgccgc ggtcgtcagc gccatcgccc aaggtggaat
      241 catcaccttc gactgcggtc cagctcccat cgtcattacc ctcgaacaaa ccgcaaaaat
      301 cttcaatgat accggaccca atatcgtcat cgacggcggt ggcctcgtca ccctcgatgg
      361 cggtggccaa cgccgaatct tgtatatgaa cacctgcgac gaggcgcaaa aatggacgac
      421 cccccattgc caaaaccagg atcatccgcg gctgacggtc cagaacatca atttttccaa
      481 cggcaactcc aaaaacgaaa cgcaatacga tggcgggggt gccatctggg tgcgcggcgg
      541 acgcttcaag ctcgtcaata ctcgcttttt gaacaatacc tgcgccgata cgggccctga
      601 cctcggcggc gccgccgtcc gcgtcctcag ccaattcgaa aacaaacccg tttatgtcgt
      661 gaattcgacg ttcgggggtg atgccaaccg tggaggcatc tgctcgaacg gtggcgccat
      721 cagcagcatc ggcgtgtcat ggaccatcat caacagcctc ttttcctata accgcgccat
      781 tggtcatggg gccaaccccg cccaaccggg cacccccggg ggcggcagcg ggggcgccat
      841 ctacaacgac ggcaacacca tgacgctatc gctttgcggt acacgaatcg agcataatca
      901 ggtaaatgct cacggaagcg ccatcttttt cgtgtccaat gaccattccg gcgatatctg
      961 gatcgatcgc tctgtcatca aaggcaatac aggcggctcc tggtatccaa cctaccctca
     1021 aatctccaac cactccgata cacccatcca cgtcaccgac tccgtcatcg aagagtaaat
     1081 ccctttcttt tcccgcacat taccatcgat tcagccccga tcgaaccacc gtaccgaagc
     1141 catcgttccc tctgccgcaa gcggtaaaac cgctctccga tacccacttg atattttggg
     1201 atggcacgac taccacattt tgtgctacca gcgtttttct cttttcatgc gattgtctcg
     1261 gcttggattt tcatggccct tcgtggctct tttcttggtc gcgccccaag cagccgctga
     1321 tcctgtcgaa cctccggtcc gccaaacccc tgccataagc tcttggcccg cgggattgca
     1381 agctccccaa gccgttgtca tccccctgga aatcgtggtt ggtgacgacg gaaccgttcg
     1441 cgaagtccat gtattgtcgt ccccctccga ggcctgggcc gaagcggcgc gcgccgaagc
     1501 catgacctgg gtatacactc ccgcgagggt gcagggggtg gcgcgaacgg ctcgcatccg
     1561 cgaccaagtg cgttttgaac cggaagccca agggacaggt caagagcaaa caccagccgt
     1621 tgacgctacg ccccctccca atgcttcccc ttccctggct tcttccgctc ccgaacccga
     1681 aacatcggca tccgcctcct tgcccacgga acccattccg gtcctcgtca ccggaaaagc
     1741 gcgtccacgc gcggcttctg acgtcgttcg caaccgcgaa gccctgtccg cggctcccca
     1801 tcgcgatggc agcgagctgc ttttaacggt tcctggcgta tttctgacgc aacatggagg
     1861 ggagggcaaa gcccatcaga tattcttacg agggtatgat gccgttcacg gccaagacct
     1921 cgaaatatgg gccggcggcg ccccggtcaa cgatgtttcc aacgttcacg gtcaaggcta
     1981 cgcggacctg cattttttga tacccgaagc ggttaatgaa gtccaggcct cggctggccc
     2041 gttcgatccc cggcaagggg atttcgccgt cgcgggtagc gtgaggtaca aactcggatt
     2101 ggcggaagaa gggtttctcg ttcagcaagg gattggttcc cataagggcc gacgcaccct
     2161 cctggcgtat cgtcccgaag gcgaaaacga ggaaacgttc accgccttcg aatcgtattc
     2221 cactgatgga ttcggtcctt cccgtgcggc gcaacatatg tcgtggatcg gacaggggct
     2281 gcttcctttg ggagggcacg cccaccttcg ggttcttggg tcaacgtatg ccggtcgttt
     2341 cgattccgcg ggtgtcattc ccctcgcctg gatcgaaaac cggggagtgg atcgcttcga
     2401 gccgtggatt cgcggacagt cgggacaatc ctctcgcaca caattcgttg cggaattctt
     2461 tcaacacgac gatgcgggca tggaatggac cgttgctccg tttttcatcc ttcgcgggct
     2521 cggattgcgt caaaacttca cgggatggct cgacgacccg gtgcgtggag atacgacaca
     2581 gcagcgaaac gatgccacga cccttggagc tacggcgcat ctgaaaaaca gaatcacggt
     2641 gttttcggag gacgatcaga ttgaagtggg cgtggtgggc agaaccgacc gcatcgagca
     2701 gagccagcga gcaattgctt ggggaacagg aagggaaacg gagacggtgg tcgatgcttc
     2761 ggtgcttgcg aaccatgtgg gcggatatct ggatgcatcg ttgcggccga tacgacgagt
     2821 gaccgtgcgg gggggcgtac gtctcgatgg gttgtcatac caggtcgacg atcgagccac
     2881 gaccggcagc cgctctgccc aggggcttcg gatgagcaag cgggcgatgg tggacgtggc
     2941 agccgcaacg gggctacatc tgatggcgag ctatggggaa ggtttccgct ctccccaagc
     3001 gcgcagtctg ggggaaggag agacagcgcc atttacaacg gtccgcgcgt tcgaaagcgg
     3061 cgttcggttc aatgcggaag aacgttttca ggcccaagca gcggttttcc aatcacgcct
     3121 ctccaaagat ctcgttttcg atcatgccac gggaagaaat gaaaccgtcc cgggaaccac
     3181 ccgttcggga atggccctgg atctggtcgt tcaacctcga ccatggctat tttccatggc
     3241 gagtctgacg gtcgttcgcg cggtttttcg cgaatccgag gggatttacg aaaaaggatt
     3301 tcttcttcct tatgcgccgc aggtggtcgc ccgatccgac acggcgtatc gtccggtggt
     3361 agggtcgatg gcgggagccg atgtgcgggg gcatctgggc gttgggctca cgctgttggc
     3421 gagaaggccg cttccttatg gcgagatggg gcacgacgtt tttcttgcgg atgcgtcagc
     3481 gcgcgcgcga tggaaagcgg tcgaacttgc cctggaggta ttcaatctct ttgatactgc
     3541 ctggtttgat ggggaattcg tatacgcctc gcgggaaaca cgaaacgccg cggctagcct
     3601 cgtgccacaa cgacacgtga ccgcaggacc accacgcacg ctttggctca caggagcgct
     3661 tcacttttga tgagggacga acccgcgaga cacctaagaa agataactgc catgaaagca
     3721 aaatcattct cgttctcgtt gctgcttggg ctggtgagca cgacggcatg gcttgcgctc
     3781 agcctcggcg cggtatcgct ggcaagcagt ggctgtgggg atagtgcgga ggaaaaaacc
     3841 gccggcaagc gcgtcaccct tcgaacacgc gtcgagtccg ccgacgatct atccaaacca
     3901 acgctcaacg ccagaggatg gagcgtcacc tggtcgcggg tgctcgtatc ggtatcgtca
     3961 ttgtactatt atgacggcgc gccgccctcg ctggcgcgct tgccctcgcc ttccctttgg
     4021 cagcgctggt tcggcatttc tgtcgcccat gcgcatcccg gacactatca ggcaggcaac
     4081 gccatgggcc agatgctcac ggccgcctcc ttcgatctgg tgtccgggcc gaccgacctt
     4141 gccgagggcg aaggcgtcac ggg
//
LOCUS       DAOSOR010000526         4154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_21523_length_4154_cov_4.048061, whole genome shotgun sequence.
ACCESSION   DAOSOR010000526 DAOSOR010000000
VERSION     DAOSOR010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21523_length_4154_cov_4.048061"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1051)
                     /locus_tag="PK710_20960"
     CDS             complement(167..1051)
                     /locus_tag="PK710_20960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12261.1"
                     /translation="MSLSKPSTPLIFSAAVVFLGCAGEVADAADAETSSTKAWLVVEQ
                     SSSLSDVPTVRSHASLWFLRIAEEKDFDAATHLVSARLQLPPVGTCQPAGVPDDQIAS
                     ASISRVELAYAGDVHVRTQRASIPLTVRFFPNVADLVSGVMYTIHDLNDVQAPFDGWL
                     SLTATGSGEFEPLEASTRPPPLPEKLSINGVTLPLVDTVVPREAPIWLGWAPDSDGDI
                     IYIDIDPVPSASVDRIRCAFSDTGLAEIPRQAIPDTTEMMIAVHRSRDVALRSDRGEV
                     GMAHFDLAVTSRVTFASP"
     gene            complement(1115..2170)
                     /locus_tag="PK710_20965"
     CDS             complement(1115..2170)
                     /locus_tag="PK710_20965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12262.1"
                     /translation="MPRSSGMLLQGLALLVTIAVCSNCTTASHLIVSHDEYSAYRKVR
                     TSTQLDKRLAASHAYLSTYPSGRWARSVKPWFDRAELRYWLLIRDTPSGLATYMQTLP
                     DGPHAKEATDALLAYRERQAAARKELLDLRAAMTEQRLNELALQREEAMNLFTAWLGR
                     FLAIESWGQTTSHLPHDTIFAWRIDPPQGTCHDDRCIKSLHQPYQVPGGGEEADRLLV
                     LDIEFLLKNGMLHEARLRGPALFSRLFEVGAKRNVRPERPADRIDAIAFSLDVISGAI
                     EARLPANRCTLPAVANVVLHRSCDGWTVQAIVADSAEDDDIVSIIGPGSSDSPSHGDA
                     ETPAAASPSSLSSPTYR"
     gene            complement(2176..2568)
                     /locus_tag="PK710_20970"
     CDS             complement(2176..2568)
                     /locus_tag="PK710_20970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12263.1"
                     /translation="MKRPLARQWEKLLLAALLLTFIIAPTPGDIGGCGQQAQLLDAPA
                     FFANKRAIDCQRCNECSFVFQSCYEACDPYAPLPDEFPTGCFPLVHDGEVCLHALHNA
                     SCNDYSAYMTDNLSIRSTPSECNFCPLR"
     gene            complement(2565..3218)
                     /locus_tag="PK710_20975"
     CDS             complement(2565..3218)
                     /locus_tag="PK710_20975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12264.1"
                     /translation="MARPSGNRQFPLPPRFRSLPQTVCLTACSLLVSLFPLPTYAHPR
                     SAADVPEPGPYIHFLSTLSIGDSLRFNNPFRLSHQLGPSGESLSRTPLYSNFGLATAF
                     GDPNGWQHGVNLQWNQALSGLPQHVITPAYLLLLDGSRPWLAFSRVGLPIVLNPNPNV
                     GGELSFGAAYLFTASLGLQTELVGNVFYGAATWEKQITAIPMLSLQLGLIIDLEVLP"
     gene            3597..4067
                     /locus_tag="PK710_20980"
     CDS             3597..4067
                     /locus_tag="PK710_20980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOT12265.1"
                     /translation="MRISCPSCQSSYKIADEKVQGRTVKVRCRKCGLTIHVNEQGVMQ
                     EGGAAGKTAADAGGAEAQFSVLVAEGDQRDMSLADIVAAYNSGVITADTYVWSEGQPD
                     WMPLGEVQVAIDAINAASEAVDKATAGQPTAEPAPPLVTAEFSTKVVFVMVFVP"
BASE COUNT          899 a         1007 c         1424 g          824 t
ORIGIN      
        1 gcctgggaaa attcgcatgc ctgttcatac aattgtgctg cccgcgcgtg catacccgcc
       61 tggatagcca gggccgccgc atccaaaaga cgcgcggagg cttccaacac atcgatctgt
      121 cgaacgattt gggccgaggc ctcgtcttgc atggacgcca tctcgactac ggtgacgcaa
      181 acgtcacgcg cgacgtcacc gcaagatcga aatgcgccat gcccacctct ccccgatccg
      241 atcgcagggc cacatcccga gaacgatgaa cggcgatcat catttcggtg gtgtcaggga
      301 ttgcctgtcg agggatttcc gcaagcccgg tatccgaaaa tgcacaacga atgcgatcca
      361 cggatgcgga aggcacaggg tcgatgtcga tgtagatgat atccccgtcg gaatccggcg
      421 cccatccaag ccatatcggc gcctcgcgcg gcaccacggt atcgacgagc ggcaaggtta
      481 cgccattgat ggacaatttt tcgggcagtg gcggaggccg tgtggaagct tccagcggtt
      541 cgaattctcc ggagcccgtg gccgtaagcg atagccagcc gtcgaagggg gcttgtacgt
      601 cgttcagatc gtgaatcgtg tacattacgc ccgagacgag atcggcgacg ttggggaaaa
      661 atcgaactgt caaggggatg gacgctcgtt gggtgcgcac gtgaacatcg cccgcataag
      721 cgagttcgac acgggaaatg ctagccgagg caatttgatc gtcgggtaca ccggccggtt
      781 gacaggttcc gacaggcggc agttgcaagc gagcgctcac gagatgggtg gcggcgtcga
      841 agtccttttc ttccgcaatg cgtaaaaacc aaagagaagc gtgggaacgc accgtcggaa
      901 cgtcggacag ggaagaggat tgttctacca ccaaccaggc cttcgtcgac gatgtctcgg
      961 cgtcggcagc atcagccact tcacccgcgc agccgagaaa cacgacggca gcggaaaaaa
     1021 tcaaaggggt ggatggtttc gacaacgaca ttcgaatcat agcctacgcg gcaggatggg
     1081 tgggggcaag cctttgacac gctttgcgtg tatgtcaacg gtaagttggc gaagaaaggc
     1141 tggatgggga cgcggcagcg ggtgtttccg catcgccatg ggaagggcta tcggaggagc
     1201 cagggccgat gatggagacg atatcatcgt cttcggcgga atcggcgacg atggcttgga
     1261 cagtccatcc atcgcaggac cggtgcaaga cgacattggc gacggcaggc agggtgcatc
     1321 ggtttgcggg aaggcgagct tcgatggcac cgctaatgac atcgagggag aaagcgatgg
     1381 cgtcgatgcg gtcggcgggt cgttccgggc ggacgttgcg tttggcgccg acttcgaaaa
     1441 gacgggagaa caaggcgggg ccgcggaggc gggcttcgtg taacataccg tttttcaaaa
     1501 gaaattctat gtcgagcacg agaagcctat cggcttcttc gcctcctcct ggcacttggt
     1561 agggctgatg cagggatttg atgcatcgat catcgtggca ggtgccctgg ggggggtcga
     1621 ttcgccaggc gaagatggtg tcatgaggca ggtggctggt tgtttgtccc cagctttcga
     1681 tggcgagaaa gcggccgagc caagcggtga acagattcat ggcttcttcg cgttggagtg
     1741 ccaattcgtt caggcgttgt tcggtcatgg cggcgcgcag gtcgagcagt tcttttctgg
     1801 cggcggcttg tctttctcgg tacgcgagca gcgcgtcggt ggcttctttg gcgtgggggc
     1861 cgtcaggcag ggtttgcatg tacgtggcaa ggccggaggg ggtatctcga atcagcagcc
     1921 agtagcggag ttctgcgcga tcgaaccagg gtttaacgct gcgagcccag cgtccggaag
     1981 ggtaggtgga aaggtacgcg tgggaagcgg cgagacgttt gtcgagttgt gtggacgttc
     2041 tgacttttcg gtaggcggag tattcatcgt ggctgacgat cagatggctg gccgtcgtgc
     2101 agttgctgca gactgcgatg gtgacgagca aggcgaggcc ttgtagcagc atgcccgagg
     2161 agcgtggcat ggtcttcatc gaagcgggca gaagttgcat tcggaaggtg tgctcctgat
     2221 gctgaggtta tcggtcatat acgcgctgta gtcgttgcat gaagcgttgt gcagggcatg
     2281 caggcatact tcgccatcgt gaacgagggg gaagcatccg gtgggaaact cgtcaggcag
     2341 gggggcgtac ggatcgcagg cttcgtaaca ggattggaaa acgaaagagc attcgttgca
     2401 tcgttgacag tcgatggctc gtttgttggc gaaaaacgcg ggagcgtcga gaagttgggc
     2461 ttgttgtccg caaccgccga tatcaccggg ggttggggcg atgatgaacg tcagcagcag
     2521 tgcggcaagc agcagctttt cccattgtcg agccaggggt cgtttcatgg cagcacctcg
     2581 aggtcgatga tgagtccaag ttgcaaggag agcatgggga tggcggtgat ttgtttttcc
     2641 caggttgcgg cgccataaaa aacgttgccg acgagttcgg tttgcaggcc cagggaggcg
     2701 gtgaaaagat aggctgcgcc aaaggaaagc tcaccgccga cgttgggatt ggggttgagt
     2761 acgatgggta atccgacgcg gctgaaggcg agccaggggc gggaaccgtc gagcaacagc
     2821 aagtaagcgg gggtgatgac atgctggggc aggccggaga gggcttgatt ccactgaagg
     2881 tttacgccgt gttgccagcc gttggggtcg ccgaaagcgg tagcgaggcc gaagttcgaa
     2941 taaagcgggg ttcgggacag cgattcgccc gatgggccga gttggtggga cagacggaat
     3001 gggttgttga agcgcaggga atcaccgatg gataacgtgg aaagaaagtg gatatagggg
     3061 ccaggttccg gaacgtcggc ggcggagcgg gggtgggcgt aggtgggcag aggaaaaagg
     3121 ctgacgagca aggagcatgc agtcaggcag accgtttgcg gaagggagcg gaaacggggc
     3181 ggaagcggga attgccgatt tcctgaaggg cgagccacgg gaacgaatct cgataggtag
     3241 cgggctgcgg gcctcggcag ccggggcgaa ccatgccgtg gtctgggcgc ggccgcaagg
     3301 ggagcatttg gaaacgaact ttgcccattg tgggatttgt cttcatgata gcgactgact
     3361 ttgctggtct gctgatggaa accagtctga tcatgaaacc aacagatggg ttgcgttctt
     3421 gactgtgcta ttcttacata ttcgtgtagt tcaaccccaa atcccatggc ccgtggttcg
     3481 gcttaccaat tttttctcga cacgggttac ataatggaag acagaggttc cgtttcccca
     3541 ccgcgagtcg gtacatcgtt ctttcccgcg tgtggttttc caggtggtcg caaacaatga
     3601 gaatctcctg cccctcttgc cagtcaagct acaagatcgc agacgagaag gtccaaggcc
     3661 ggaccgtcaa agtacgctgc agaaaatgcg gattgacgat tcacgtcaac gaacaaggcg
     3721 tcatgcaaga agggggggcg gcgggaaaga ctgccgccga tgcgggtggc gccgaggccc
     3781 aattctccgt gctcgtagcg gaaggagatc agcgagacat gagcctcgcg gatatcgtgg
     3841 cggcctataa ctcgggtgtc atcacggccg atacgtacgt atggtccgaa ggacaacctg
     3901 attggatgcc gctgggggaa gtgcaagtgg ccatcgatgc catcaacgcc gcgtcggaag
     3961 ccgtggacaa ggcgaccgcg gggcagccga cggccgagcc agcaccgccg ctggttacgg
     4021 cggaattttc aacaaaggtg gtatttgtta tggtatttgt gccataataa ccaacgattg
     4081 caccgcccac atattctgca gtattggcgg tgaaggtgct gcctgttact gtgagcgtgc
     4141 cagcaaagtt ggta
//
LOCUS       DAOSOR010000527         4136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_21660_length_4136_cov_3.768439, whole genome shotgun sequence.
ACCESSION   DAOSOR010000527 DAOSOR010000000
VERSION     DAOSOR010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21660_length_4136_cov_3.768439"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="PK710_20985"
     CDS             complement(<1..888)
                     /locus_tag="PK710_20985"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOT12266.1"
                     /translation="MDTARERIVVTGLGMVTPCGTGVTETWDNLLAGRSGIGLITAFD
                     ASEFAVRIAGEVKDWDPSVHIPKKKVRELGRFAQFAVASAQLAVRDAGLELTEQEREN
                     AACVMGVGMGGLEGLERVSEVIREKGPSKVSPYAVPTIAMNMAAGQISIALGIHGPSF
                     CTASACASGAHAIGEALMLLRAGAASVAIAGGAEATITPTGIAGFQAMFALSRRNDDP
                     KGACRPFDTQRDGLSPPKEQRSWCSSRSLAPSVAALASMRNSRDTVPRATLIIPFNPR
                     STAEVPWHRCVGRCRMPDSL"
     gene            complement(946..1194)
                     /gene="acpP"
                     /locus_tag="PK710_20990"
     CDS             complement(946..1194)
                     /gene="acpP"
                     /locus_tag="PK710_20990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOT12267.1"
                     /translation="MADKRDIAAEVTAIIKEQMDVDEKDIKPESTFIDDLGADSLGLV
                     ELVLAFEERFEIDIPDEDTEKIRTVQDAIDYIEKHAKK"
     gene            complement(1184..1396)
                     /locus_tag="PK710_20995"
     CDS             complement(1184..1396)
                     /locus_tag="PK710_20995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12268.1"
                     /translation="MNNASRCKQRDGGGSKQLDGFRGARYAVRPLSQDTVMTGSAGVA
                     WLDEGPRRLDDDNELNTTCPEGPHGG"
     gene            complement(1402..2271)
                     /gene="fabG"
                     /locus_tag="PK710_21000"
     CDS             complement(1402..2271)
                     /gene="fabG"
                     /locus_tag="PK710_21000"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOT12269.1"
                     /translation="MLGGREDGGQLSIFDGGIRGGVTAKLRVPLSWGASRAKERGMFR
                     LDDKVALVTGSSRGIGRAIAVGLAAQGARVFVNYLSGKQQAEQVVMEIEAAGAKAEAL
                     GFDVADSKAVDMAVTQVVERAGRLDIVVANAGISKDGLLMRAKDEDLQSMLEVNLRGA
                     IALARSSIKPMLRARRGRIILVSSVVGEMGNVGQTFYAASKAAMLGVTKSLARELGSR
                     GITVNAVAPGLIDTDMTAGMSEQMRGAMVNAIPLGRFGQAGDVAAAVAYLASDEAGYV
                     TGHVLRVNGGMYG"
     gene            complement(2754..>4136)
                     /locus_tag="PK710_21005"
     CDS             complement(2754..>4136)
                     /locus_tag="PK710_21005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12270.1"
                     /translation="PGDASGDQGDASGDQGSVHETGGPCNPRSCLDLSAQCGTVLDGC
                     GGTVDCGSCPTGQTCGGAGPNQCGVGECTPKTCVQLNAACGLVSDLCGNVLSCGECAK
                     PEVCGGAGIENQCGCTPISCASVKAECGTLSDGCGGVAHCGSCPKGMVCGGKEQNRCG
                     PEPCTPKTCASIGANCGAYADGCDGVIDCGTCVAPQTCGGGKDPFRCGCTPTTCVSHN
                     AECGTVADGCGGTLSCGTCKYGTCQGNRCVCAPVTCASSMAECGTIDDGCGKSLSCGS
                     CKAPRQCGAAGVANQCSCTPAICPPIFADSFESGNMGTKPTGWQIWHNCHADNAWFVE
                     IAGSPAPGGGFQNLRFQTTKFSGSCSSPGAYAQTDYLPVIAGRNYQIESWSRNWGSHS
                     KIDALFYSAQQEYISMKSKDFPADSGSYKQNPSLLVKAPANASFVRIRVALQDSGAAL
                     DFDRLELFEE"
BASE COUNT          890 a         1474 c          925 g          847 t
ORIGIN      
        1 caacgagtcc ggcatcctgc atcgccctac gcatcgatgc catggcacct cggccgtcga
       61 gcgcgggttg aacgggatga taagcgtcgc acgaggaacc gtatcccgtg agttccgcat
      121 agatgcgagc gccgcgacgc ttggcgcgag tgagcggctc gagcaccagg accgctgctc
      181 cttcggaggc gacaacccat cgcgttgcgt gtcgaacgga cgacacgcgc ctttggggtc
      241 atcgtttctt cgcgacagag caaacatggc ctgaaaaccc gcaatgcccg tgggggtgat
      301 ggtagcttcc gccccgcccg ctatcgccac ggaagccgct cccgcgcgaa gaagcatcaa
      361 agcctcgcca atggcatggg ccccggaagc acaggccgac gcggtacaaa agcttgggcc
      421 atgtatccca agcgcaatcg aaatttgtcc cgccgccatg ttcatcgcaa tcgtgggaac
      481 cgcataagga ctgactttcg aaggcccttt ttcgcgtatc acctccgaca cacgctccaa
      541 accttccaat ccccccatgc caactcccat cacacaagcc gcgttttccc gctcctgctc
      601 cgtcaactcc aatcccgcat cccgcaccgc aagctgcgcc gatgccaccg caaattgcgc
      661 aaaacgcccc aactcacgaa cctttttctt gggaatatga accgaaggat cccaatcctt
      721 cacttcaccc gcaatacgaa ccgcaaactc actcgcgtca aacgccgtga tcaacccaat
      781 tccactgcga cccgcaagca ggttatccca ggtttccgtc acaccagtcc cacatggcgt
      841 caccatgccg agacccgtca cgactatccg ttctcgtgct gtatccatga ctgctcgtcg
      901 gaaagtgcgt cgcgcacaaa ccccggagcg acggggccca caaaactatt tttttgcgtg
      961 cttctcaata taatcgatgg cgtcctgcac cgtgcggatt ttttccgtat cctcatcggg
     1021 gatatcgatt tcgaaccgct cctcgaacgc caacaccaac tccaccaacc caagagaatc
     1081 cgctccaaga tcgtcaatga atgtcgactc gggcttgatg tccttttcgt cgacatccat
     1141 ctgctctttg atgatcgccg tcacctcggc cgcaatatca cgcttatccg ccatggggtc
     1201 cctccggaca cgtagtgttc aattcgttgt catcgtcgag tcgtcgtggc ccttcatcca
     1261 gccaagccac gcccgccgat ccggtcatca ccgtatcttg tgataacggt cgcacggcgt
     1321 agcgcgctcc tcgaaaacca tcaagctgtt tcgaccctcc accgtctctc tgcttgcaac
     1381 gcgatgcgtt attcacgact ttcagccgta catcccgcca ttcactcgca acacatgccc
     1441 cgtcacgtat cccgcttcgt cgctcgccaa ataggccacc gccgcggcta cgtcccccgc
     1501 ctgcccaaat cgacccagcg gaatcgcatt caccatcgcc ccgcgcatct gctcactcat
     1561 ccccgccgtc atgtccgtgt cgatgagccc cggagccacc gcattgaccg tgattcccct
     1621 cgaacccaac tccctcgcca aactcttggt cactccaagc atcgccgctt tcgacgccgc
     1681 gtaaaacgtc tgccccacgt tgcccatctc gcccacaaca ctcgacacca aaatgattcg
     1741 acctcgccgc gctcgcagca tcggcttgat cgacgacctg gccaacgcaa tcgccccccg
     1801 aaggttgact tccaacatcg attgcaaatc ttcatccttc gcacgcatca gcaatccgtc
     1861 tttcgatatc cccgcattgg cgaccacgat gtccagacgc cccgcgcgct caacgacctg
     1921 cgtgaccgcc atgtcgaccg ctttgctatc ggctacatcg aaaccaaggg cctccgcctt
     1981 tgctcccgcc gcctcgattt ccataaccac ctgctcggcc tgctgcttgc ccgagagata
     2041 gttcacgaac actcgcgccc cctgggccgc aagtcccacc gcgatcgccc ggccaatccc
     2101 tcgggatgaa cctgtcacca acgccacttt gtcgtccagc cgaaacatgc ctcgctcttt
     2161 cgcacggctt gctccccaac tcaagggaac acgcaacttc gccgtcacac caccccgaat
     2221 tccaccatcg aaaatcgata gctgcccacc gtcttctctc cccccaagca tgaagcaaac
     2281 ggctccgacc agccttgcac cctcgggttg ccgttttccc ttcttccccc cgtcgcctca
     2341 tgcctcgatg gttctgcccc ctttccatgt cattgccatc gattgaaata gtggcagcta
     2401 cgtccacgac gatgatgtcg ccaggatggg tgaagtatac gattacgtat atttgtgact
     2461 tggagaaatg gcgtgatata tgttgtattc tccaaaaaat atagaatatt tttatcttta
     2521 atgacaaccc atcggcaaat tttgagtata tccgttaatg gataattggc attccagcca
     2581 tcatccggat cgcaaaacca gctccccgca tcaaccgcat cacttccgta ccagggcccc
     2641 cacaacgcga tcggcccgaa ggggccgcgc gacaccctca tccccaccct gcctcaccca
     2701 caacgaaaat cacatgggtg ccgcggtgat ggatactgcc ctctcacgct ccgtcattct
     2761 tcgaatagtt cgagcctatc gaagtcgaga gccgcccccg agtcctgtaa cgcaacacga
     2821 atccgaacga acgaggcatt cgcgggcgct ttcaccagta acgatggatt ctgcttatac
     2881 gatcccgagt ccgcaggaaa atcctttgat ttcatagaga tatactcttg ttgcgcgctg
     2941 tagaacaacg cgtcgatctt gctatggcta ccccagtttc tcgaccacga ctcgatctgg
     3001 taatttcttc ccgcgatgac gggcagatag tccgtttgcg cataagcgcc cggggatgaa
     3061 caggaaccac tgaacttggt cgtctggaaa cgaagattct gaaaccctcc ccctggagct
     3121 ggagacccgg cgatttcaac gaaccaagca ttgtccgcat gacagttgtg ccaaatctgc
     3181 cagcccgtag gcttggtgcc catgttgccg gattcgaaac tgtccgcgaa aatgggaggg
     3241 catatagccg gtgtgcaact gcattgattc gctacccccg cagccccaca ttgcctcggc
     3301 gccttacatg aaccacagga aagactcttc ccacagccat cgtcgatcgt gccgcattcc
     3361 gccatgctcg aggcgcaggt gaccggtgcg caaacgcatc gattcccctg gcatgttcca
     3421 tacttgcatg tcccacaaga aagagtcccg ccgcagccgt ccgcaacagt gccacattca
     3481 gcgttgtgcg atacacacgt ggttggcgta catccacagc gaaacggatc ctttcctccc
     3541 ccacaggttt gcggcgccac gcaggttcca caatctatta ccccatcgca cccgtccgcg
     3601 tatgccccac aattcgctcc gatactcgcg cacgtcttcg gagtacatgg ctccgggcca
     3661 caacggtttt gctccttccc tccgcagacc atccctttgg gacagcttcc acaatgggcg
     3721 accccaccgc aaccatcgga aagcgttcca cattccgcct tcacggacgc gcaagagata
     3781 ggcgtgcagc cacattgatt ctcgatcccc gcccccccac acacctccgg cttcgcgcac
     3841 tcgccgcacg acaacacatt gccacataga tccgatacca aaccacaagc ggcgttgagc
     3901 tgcacacagg tcttgggcgt acattcgccg acgccacatt gattgggtcc cgcaccacca
     3961 caggtttggc ccgtggggca cgaaccgcaa tccaccgtac ccccacagcc gtcaagcacc
     4021 gtcccacatt gggcactcaa gtcgagacag cttcgtgggt tacacggacc ccctgtttca
     4081 tggacacttc cctgatcccc cgacgcatcc ccctgatccc ccgacgcatc cccagg
//
LOCUS       DAOSOR010000528         4134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_21678_length_4134_cov_3.295416, whole genome shotgun sequence.
ACCESSION   DAOSOR010000528 DAOSOR010000000
VERSION     DAOSOR010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21678_length_4134_cov_3.295416"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="PK710_21010"
     CDS             complement(<1..972)
                     /locus_tag="PK710_21010"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase GenX"
                     /protein_id="HOT12271.1"
                     /translation="MNVMSPHDLRTMEHGARVRVGGRVRAQPHGRWQIADAFDTVAVV
                     AESSLKEGCLVIVEGRWDGECIDEAVVVDVSEPMADQFHESARLLDRGVGWALQRRAT
                     ILGVIRNYFENQGFIEVETPVRVPCPGMDLHLDAFDSGPAALITSPEYQMKRLLAGGI
                     PKLFQLVHCFRDDEQGDLHNSEFTMLEWYRAFAGIEEMIRDTEGLIEHVARQVTGKAT
                     VRWRGRELDVSAPFERISVLQAFARHALLDRDEVLRLASAEEDTYFGLLVEKVEPALA
                     ASAHAVVLYDFPSVHASLARRRVDDPMVCERFEVYVGGVELCNGFGEL"
     gene            complement(969..1577)
                     /locus_tag="PK710_21015"
     CDS             complement(969..1577)
                     /locus_tag="PK710_21015"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT12272.1"
                     /translation="MKKKRSLEQQKTKRARILAAAAEVFATRGFQEAHISHIAQTAGV
                     ADGTIYLYFPSKESLLVALFEDSVSRFFRQLDDELTSIAAPRDKLLRMVALQLGLLDR
                     ERGLAELLTVNLRQSSRSMRELLKPHFTAYLDRLERVLIEGQNAGVFRKDLSARVVAR
                     AIFGALDGVTLTWALGGGHEGGLVKTAETVGKMLLDGLASNS"
     gene            complement(1624..1878)
                     /locus_tag="PK710_21020"
     CDS             complement(1624..1878)
                     /locus_tag="PK710_21020"
                     /inference="COORDINATES: protein motif:HMM:NF013480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HOT12273.1"
                     /translation="MGLSTIISLSQQALVLSLAVVLPVLAVAAVVGLVVAVLQAATQV
                     QDPTLAHLPRIVAVVAALAAFGPWMGREIAAFALRVFSGG"
     gene            complement(1880..2371)
                     /locus_tag="PK710_21025"
     CDS             complement(1880..2371)
                     /locus_tag="PK710_21025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12274.1"
                     /translation="MRRIRFGVLVVAFVALWAGACSNQGEGERCSLDNNNEDCASGLR
                     CQKIEGQDSPLCCPPPDRPSTVAACIPGQVYRPDSGLTDQGLPDSIVTEAASDSPGDE
                     PQEASVEDVSLDVAEDVEPVETGIETSLDVTPDVDDDETGLEASVDAEKDVVEEAEAD
                     AGG"
     gene            complement(2459..3151)
                     /gene="pgsA"
                     /locus_tag="PK710_21030"
     CDS             complement(2459..3151)
                     /gene="pgsA"
                     /locus_tag="PK710_21030"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOT12275.1"
                     /translation="MTEPSRHSLSPPSTPSPASRRIRRKQRRRSLREDALNLPNLLTM
                     GRILVIPLVLYLLDRGSPFDCYLATLVFVLAAITDFLDGYLARKRNLVSVLGKFLDPL
                     ADKLIVTATLVWLVTMGRVPAWLVIILIGREITITALRGIAMTEGLVIAAGEGGKSKT
                     ALQMVGLVCLILGYPYHVDLIVVDFGVVDLVMVGRALIYCSLVFSIVSAFQYGKFFVD
                     AVEAKAKRAPSG"
     gene            complement(3214..>4134)
                     /locus_tag="PK710_21035"
     CDS             complement(3214..>4134)
                     /locus_tag="PK710_21035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12276.1"
                     /translation="GLRIFPAVLWAGPLAIIAWDIYRKRRLQRSYLKFLAGGAAAVLV
                     LVPLSLHVTGGVHSYEEFMHHIKVHQNTALTNHVGLHTVIKASPEGRMKYARNNALLD
                     PFERWKKQRNDRFEKYKVLWGALFLSFGGLLFFALRRTKALWIGMGLSLILVVSAAEA
                     TCYYWSIWVLAAMLSRLKRQMEWPLLAVFLGVFAFAGFKILLTQMPDNSLTAFSISVS
                     VVAALYYYYHETLLRFARSNGSMEIAIIGLAGASQVFSNQFYFIDDKFTAISALYVAW
                     TVAFVLAFFRWPRAQLGLNTLPVRSESPGA"
BASE COUNT          998 a         1320 c          998 g          818 t
ORIGIN      
        1 cagttctcca aacccattgc ataattcaac cccccccacg tacacctcga atcgctcaca
       61 taccatcgga tcatccacac gacgtcgagc aagcgatgca tgaacggaag ggaaatcata
      121 gagcaccacc gcatgggccg acgctgccaa cgccggctcg actttctcga ctaacaaacc
      181 aaagtacgta tcttcctccg cactcgccaa tcgaagcacc tcgtcgcgat cgagaagcgc
      241 atgccgtgca aacgcttgga gcaccgaaat tctctcaaaa ggcgcgctga catccaactc
      301 ccgaccacgc caacgcaccg tggctttccc cgtcacttgt cgcgcaacat gctcgatgag
      361 gccttctgta tcccgaatca tctcctcgat tcccgcgaag gcacggtacc attcgagcat
      421 cgtaaattcg ctattatgaa ggtccccctg ctcatcgtcg cgaaaacaat gaacgagttg
      481 aaataacttt ggaatcccac ccgcaagcaa tcgcttcatc tgatactcgg gtgaggtaat
      541 caacgccgcg ggcccggaat caaaggcgtc aaggtgcaaa tccatcccag ggcaggggac
      601 ccgcacgggg gtctcgactt ctataaagcc ttgattttca aaataatttc gaatgacccc
      661 gagaattgtt gcccgacgtt gcaacgccca gcctaccccg cgatcgagca accgggcgga
      721 ctcatgaaac tgatccgcca tcggctccga cacgtccacc accacggctt cgtcgatgca
      781 ttccccatcc catctgccct cgacaatcac caaacagcct tctttgagcg atgattcggc
      841 tactacggct acggtatcga aggcgtccgc gatttgccat cgtccatgcg gttgcgctcg
      901 aactcgcccg cctacacgga ctcgagcccc atgctccatc gtccgcaagt catgaggaga
      961 catcacattc atgaattcga ggccaatccg tcgagcaaca tttttccaac cgtttcggcc
     1021 gtcttgacaa gaccgccctc gtggccccct cccaaagccc aggttagcgt caccccatcg
     1081 agcgctccaa aaatggctcg ggcaaccacc cgtgcggata agtcttttcg aaatacccca
     1141 gcattttggc cttcgatcag cacacgctcg agacgatcga gataggcggt gaagtggggc
     1201 ttcaatagct cacgcatcga acggctcgat tgtcgtaggt tgaccgtcaa caattccgcc
     1261 agcccacgct cgcggtccag cagcccaagc tgaagcgcca ccattcgcag cagcttgtca
     1321 cgcggtgccg cgatcgaggt aagctcgtca tcgagttgcc gaaagaagcg cgaaacactg
     1381 tcttcgaaga gcgcgacgag caatgattcc ttgctcggga aatacaggta gatggtgccg
     1441 tcggccacgc ccgccgtctg agcgatatgg gagatatggg cttcttgaaa accgcgagtc
     1501 gcaaacacct cggccgccgc tgccaagatt cgcgctcgtt tcgttttctg ctgctccaac
     1561 gaccgcttct tcttcacgtc tccgccgctg tatcgatgaa ccaaccctgt cgagaaagca
     1621 ccttcagcca ccgctgaata cgcgaagggc aaacgctgca atctcgcgcc ccatccatgg
     1681 accaaacgcc gccaatgccg ccactaccgc aacgattcgc ggcagatgcg ccaacgttgg
     1741 gtcctgtacc tgggtcgccg cctgcaatac agccacgacc aacccaacca cggcggctac
     1801 cgccaacacc ggcaacacca ccgccaagga caaaaccaaa gcttgctgcg acaacgagat
     1861 gatcgtgctg agccccatct cacccccccg cgtccgcttc ggcctcctca actacgtctt
     1921 tttccgcatc taccgatgct tccaatcccg tctcgtcatc gtcgacatcg ggcgttacat
     1981 ccaacgatgt ctcgatgccc gtctccaccg gttcgacatc ttcggctaca tctagcgaga
     2041 cgtcttccac cgaggcttct tgcggttcat cccctggtga atccgatgcg gcctccgtca
     2101 cgatggaatc cggaagtcct tgatcggtca gtccgctgtc cggtcgatag acttgaccgg
     2161 gtatgcaagc agcaaccgtc gacggtctgt ccgggggcgg acagcacagc ggggagtctt
     2221 gcccttcgat cttttggcat cgaagtcccg acgcacagtc ttcgttgttg ttgtccaagc
     2281 tacatcgttc tccctctcct tggttcgaac aagctcccgc ccacaaggcc acgaaagcga
     2341 caaccaaaac gccaaaacga attcgcctca tcgtcggtga ctccagggat gccaaggtat
     2401 tcttcaactc gcctgtccta caagaggctg tgcgccgtgt ccatgctcgt atcgaattct
     2461 atcctgatgg cgctctcttt gctttcgcct cgaccgcatc cacgaaaaac ttgccgtatt
     2521 gaaatgcgct cacaatcgaa aaaacgagtg agcagtagat caacgccctg cctaccatca
     2581 ccaaatccac cacgccaaaa tccaccacaa tgagatccac atgataggga tatccaagga
     2641 tcaaacacac gagccccacc atctgtaacg ccgtcttcga ctttcctccc tctcccgccg
     2701 cgatcaccaa cccttccgtc atggcaattc cgcggagggc cgtgatggtg atctctcgac
     2761 cgatcaaaat aatgaccaac cacgccggaa cgcgacccat cgtgaccaac cagacaagcg
     2821 ttgccgtcac aatcagctta tccgccaacg ggtcgagaaa tttgcccaag acactgacca
     2881 agtttcgttt gcgagccagg tagccatcca aaaaatcggt aatcgccgcc agcacgaaaa
     2941 ccaaagtcgc caaatagcaa tcgaacgggc tgcctcgatc caacaggtac aacaccaggg
     3001 gaatgaccaa aatccttccc atcgtcagaa gattgggaag attcaaagcg tcttcgcgaa
     3061 gcgaacggcg acgctgcttt cgtcgaattc gtcgtgacgc cggggagggg gtcgacggcg
     3121 ggcttagcga atgtctagaa ggttccgtca catctgtggt tgtaacggca gccgcgtgcg
     3181 ttggctatgg ataatgacct gaagttcgat ggatcaagct ccgggggact cggagcgcac
     3241 cggcaaggtg ttgagcccaa gttgcgctcg aggccaacgg aaaaaggcca gcacgaaagc
     3301 tactgtccat gccacgtaga gcgccgaaat cgctgtgaac ttgtcatcga tgaagtaaaa
     3361 ctgattcgaa aacacttggc tggcccccgc caagccgatg atcgctatct ccatgcttcc
     3421 gttgcttcga gcgaaacgaa gcagggtttc atgatagtag tagtacagcg cggccacaac
     3481 gctcacgctt atgctgaacg cggtcaacga attatccggc atctgcgtga gcaggatctt
     3541 gaagcccgcg aacgcgaaaa ctccaagaaa tacagctagt aacggccatt ccatctgacg
     3601 ttttaggcgc gatagcatcg cggccagaac ccagatcgac caataataac acgtggcctc
     3661 cgcagcgctg accaccagga tcagcgacaa gcccatccca atccacaggg ctttggttcg
     3721 gcgtaaagcg aaaaaaagca agccgccaaa cgagaggaac aaggctcccc acaacacctt
     3781 gtatttttcg aaacgatcgt tgcgctgctt tttccaccgc tcgaacgggt cgagcaaggc
     3841 gttgttgcgc gcgtacttca ttcgcccttc gggcgatgcc ttgatgaccg tatgcagtcc
     3901 cacatggttg gtcaacgcgg tgttttgatg cactttgatg tggtgcatga actcttcata
     3961 cgaatgcaca cctccggtga cgtgcaagct caggggaacg agcacgagaa ccgcggctgc
     4021 tcccccggcc aaaaacttga gataggaccg ctgcaatctt cgctttcgat agatgtccca
     4081 ggcgatgata gccagggggc cagcccatag aaccgccgga aatattcgta atcc
//
LOCUS       DAOSOR010000529         4105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_21864_length_4105_cov_4.177778, whole genome shotgun sequence.
ACCESSION   DAOSOR010000529 DAOSOR010000000
VERSION     DAOSOR010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21864_length_4105_cov_4.177778"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PK710_21040"
     CDS             complement(<1..229)
                     /locus_tag="PK710_21040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12277.1"
                     /translation="MGRGSKLLGISLLLMLSCVPAQAQKERTGIERTPPPTQLPRSRP
                     APVAPTVSPQPRPAPAVKRCPEATSLPSGYVC"
     gene            458..1252
                     /locus_tag="PK710_21045"
     CDS             458..1252
                     /locus_tag="PK710_21045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12278.1"
                     /translation="MPLTIRPLEIMALVLVSGCTTLGPTPMVSGQSMALNPRTHVEVQ
                     AAAVPGFFLSSAATDPEGTPTQQVAGLFEPGDWLPLKGLSAGGRWVGGGDESGHFEPM
                     IRYRTEIDEQQRVAVGLVGWGAMHEGSSAQASYEARRVALEVGADVRLTPKSRWVELH
                     GMAALAGTWLDASGSYCANAEGKGIQCDIAGPPKAHAETSSVYPAGSAGLALDLGSRF
                     ESVFHGGRLFAMVGGGWMPKVRDGEPADRAVFHSLGGGLSLALGAP"
     gene            1740..2840
                     /locus_tag="PK710_21050"
     CDS             1740..2840
                     /locus_tag="PK710_21050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12279.1"
                     /translation="MPLFERADGWSTPTRCFETPAGVRHLNESQAFELYRTIAESTTG
                     LTMNTSPEVRTIVGLRGAYPGQLIYHGNLPDRFNDTLVLLWTTKEGFRHVREFPVTTD
                     PGLFDFGRHHASFLRANRRYRLRNGLHNSYHALRMAEKSYVVRDDTNHNGHWDNDRNG
                     WLSPRKLRDHDRQGSAHNIHMAEIDGPLGHASIGIWSAGCQVIPGIANWRAFITQAFT
                     KTRDRVDYFLIDSRDIPASVWRPCTADGTPECPFRIDTLPYTSQGDTSQSGVQAWETY
                     SGSDKAYSGREITYALTVDRRGKLTIRLDYQPPVRLAVHVLEGSDPKACVAWGDKAVI
                     RDIEPGRYFLVVDTPSQTAPPAGAFTIHVSLN"
     gene            2895..4028
                     /locus_tag="PK710_21055"
     CDS             2895..4028
                     /locus_tag="PK710_21055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HOT12280.1"
                     /translation="MRDVSARFGVATLVVWTGCSTSTGTFDSPSDPILDAAAEVSTDA
                     MADGEAVGPGDAETEGSATDVYPDMDNDGMDLEAAAGDGTLLERSYVGPEGTRDYLLY
                     EPAGYDGTPRALVVALHGCSQSASHFATASGYNRLADQHGFIVVWPEQSLLANTALCW
                     NWFLPGHQRRDVGEAAVIAAIVGEVGKTHALDPKRIHAVGMSAGAAMAVVLGAVFPDV
                     FASIGSVEGCPFQGTPCVGSASVLPAATLATYVVSAMGARARPVPLFAIQGSLDINVP
                     PANGDLLVKQWVLAADVVDDNLSNQSISSFPHHVSDDSAHGYSFRRSVYVDGAGKDFI
                     EYLEVKGLGHAWPGASVSLAFSDTQGPSATEGSWHFLEAHPMP"
BASE COUNT          788 a         1139 c         1267 g          911 t
ORIGIN      
        1 tgcacacata accgctggga agtgatgtcg cctcaggaca ccgcttcaca gcaggtgcag
       61 gtctgggctg tggacttacc gtgggcgcaa caggtgccgg tctggaccgt ggaagctgcg
      121 tgggcggggg cgttcgttct atcccagtgc gctccttttg cgcctgcgcc ggcacgcacg
      181 atagcatcaa caacaacgaa atacctaaca gcttagaccc gcgacccatc cttcgactcc
      241 ttcggtccgg ttagtataca accgttccga gaaatgttcg acggcttcat ttcggttttc
      301 ttccaagcct tgaccttgtt tggcggaaca tgaattctgc cacacctgtt gtcgaaaact
      361 cacaacaccg cagtcccaga tatattgtaa ctacttgaat atatcaggat taatactatt
      421 tttccaccaa ggcatggagg atgctaggat gatgatcatg ccgcttacca tccgtcccct
      481 ggagatcatg gcactcgttt tggtctcggg ctgtacgaca ttgggcccca caccgatggt
      541 gtcgggacag tcgatggccc tgaatccccg aacccacgtg gaggtgcaag ctgccgcggt
      601 tcctggtttt ttcctcagct cggcggccac ggatccggaa ggaacaccca cgcagcaagt
      661 cgctggcctt tttgaacctg gagattggct tcctttgaaa gggctttccg cagggggtcg
      721 gtgggtgggc ggtggcgatg agtcgggtca tttcgagccg atgattcgct atcgaaccga
      781 gatcgacgag cagcagcgcg ttgcggtggg gctggttggc tggggggcga tgcatgaagg
      841 ctcttcggct caggcgtcgt acgaggcgcg gcgggtcgcg ttggaagtcg gcgccgatgt
      901 acgtctgacc cccaaaagcc ggtgggtgga gctgcatgga atggcagctt tggccggcac
      961 atggttggat gcttccggat cgtattgcgc caatgccgaa ggcaaaggaa tacaatgtga
     1021 catcgcaggc ccaccgaagg cgcacgccga aacttcgagc gtctatcccg cgggtagcgc
     1081 aggacttgcc ctggacctgg gttcgcgatt cgaaagcgtg tttcatggcg gaaggctatt
     1141 tgccatggtc ggcggtgggt ggatgcccaa agttcgtgac ggcgaacccg cggaccgggc
     1201 cgtatttcat tccttgggag gcggcctgag tttggcgctg ggagcaccgt gaacaccatc
     1261 ctatcgcccg tggtagcaaa ggcttgccgc atcggatgaa catgtcggca cgaacatcag
     1321 gttcgaattg cacggcgtct cctggaccgc ggccctcgat ctgcagaagc attgttttgc
     1381 tgatattgtt ctgtttcacg cccgtccatt gcgacccttc ccgacgatcc cacgccgacg
     1441 cttcgccttc ccaaacctcg ttgcccccgg ccgaagcctc tgcgagggaa aaacaacaat
     1501 atcccattaa ttcaataact tataacgatg ctgaccaagg atcggcgaaa gaaacctcct
     1561 gtggtttcgg ttatgaatcg gctttttcga acagcccacc tctgcccgag ggcgtttccg
     1621 ggcgcaacga tgatcgtacg gaaccgacaa ccgagccacc tccgccctat acctatcgac
     1681 tcgccggatg gcgggaccac catcccgaag acctggtgat gcccggatac aaggatacca
     1741 tgccgttgtt cgagcgagcc gacgggtgga gcacgcccac ccgctgtttc gaaacccccg
     1801 cgggggtccg gcacctcaat gaatcacagg cctttgaact ctaccgcacg attgccgaaa
     1861 gcaccacggg actgaccatg aataccagcc ccgaagtgcg aacgatcgtg ggtcttcgcg
     1921 gagcctatcc ggggcaactc atctatcacg ggaatttgcc ggatcgattc aatgacacgt
     1981 tggtattgct ctggaccacg aaggagggtt tccggcacgt acgggagttt ccggtaacca
     2041 ccgacccggg gcttttcgac tttggccgcc atcacgcctc atttcttcgc gccaatcgcc
     2101 ggtatcgcct tcgcaacggc ttgcacaatt cctatcatgc gctgcgaatg gctgaaaaaa
     2161 gctatgttgt ccgcgacgat accaaccata atggtcattg ggataatgac cgtaacggct
     2221 ggctatcacc gcgtaagctg cgggatcatg accggcaagg cagcgcgcac aacatacaca
     2281 tggcggaaat cgacgggccc cttgggcacg cctcgatcgg catttggtcg gcgggatgcc
     2341 aggtcattcc gggaatcgcg aattggcgag ccttcattac gcaggccttt acgaaaaccc
     2401 gggatcgggt cgactatttt ctcatcgaca gtcgggatat tccggcttcg gtgtggcggc
     2461 cctgcactgc cgatggcacg cctgaatgcc cctttcgaat cgatactctc ccgtatacga
     2521 gccaggggga tacgtcgcag tctggcgttc aggcatggga aacgtactcc ggctcggata
     2581 aggcgtattc cggccgggag atcacctacg cgctgacggt cgacaggcgg ggaaagctca
     2641 cgattcgcct tgattatcag cctcccgttc gccttgccgt ccacgtgctc gagggtagcg
     2701 atccgaaagc gtgtgtggct tggggggata aggcagttat ccgggatatc gagcctgggc
     2761 gatattttct tgttgtggat acgccttcgc agactgcccc gcccgccggt gcgttcacga
     2821 ttcatgtttc gttgaactga aaggcagagg taaagcggct ggcgcagagg gtcgaaagcg
     2881 agtaagtcat agatatgcgt gacgtttcgg cgcggtttgg cgtggcaacg cttgtggtat
     2941 ggacaggttg ctcgacatct accggcactt tcgattcgcc gtcggatccg atactcgatg
     3001 cggcagcgga agtatccacg gatgcgatgg cggatggtga ggcggtaggg ccgggggatg
     3061 ccgaaaccga ggggagcgcg acggacgtct atccggatat ggataatgac gggatggatc
     3121 tcgaagcggc cgcgggagat ggcacattgc tcgagcgctc ctacgtgggt ccggagggga
     3181 cgcgggatta tttgctctac gagccagccg gttacgacgg cacgcctcgt gccttggtgg
     3241 tggctttgca cggctgtagt cagtcggcct cgcattttgc gacggcatcc gggtacaatc
     3301 gtctagccga tcagcacggg ttcatcgtgg tgtggcctga gcagagtttg cttgcgaata
     3361 ccgccttgtg ttggaattgg tttttgcccg ggcatcagcg tcgggacgtg ggggaagcgg
     3421 cggtgattgc cgcgatcgtg ggggaggttg ggaaaaccca cgcgctcgac ccgaagcgga
     3481 ttcacgcggt gggaatgtcg gcgggggcgg ccatggcggt ggtattggga gcggtttttc
     3541 cggatgtatt tgcgtcgatt ggctcggtgg aagggtgtcc tttccaggga acgccttgtg
     3601 tcggctcggc aagcgtattg cccgcggcca ccctcgctac ctatgtcgtc agcgccatgg
     3661 gcgcacgggc tcgcccagtg cctcttttcg ccattcaggg ctcgttggac atcaatgtcc
     3721 cgcccgccaa cggtgatctt ctggtcaaac aatgggtact ggcggcggat gtggtggatg
     3781 acaacctgtc gaatcaatcg atttcctcgt ttccccacca tgtgagtgat gattcagccc
     3841 atgggtatag ctttcgacgt agcgtctacg tcgacggcgc ggggaaggat ttcattgaat
     3901 acttggaagt caaaggtctt ggtcatgcgt ggccgggggc gtcggtatct cttgcttttt
     3961 ctgacacgca ggggccgtcc gccacggaag gctcctggca ttttttggag gcgcatccga
     4021 tgccgtgagt cccgggggca tggtttgcgg ctgagccatc ccctcatttt tcccggacta
     4081 tttcccttgt tgtcgactgc cggaa
//
LOCUS       DAOSOR010000530         4094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_21944_length_4094_cov_4.061401, whole genome shotgun sequence.
ACCESSION   DAOSOR010000530 DAOSOR010000000
VERSION     DAOSOR010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21944_length_4094_cov_4.061401"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="PK710_21060"
     CDS             complement(<1..1274)
                     /locus_tag="PK710_21060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT12281.1"
                     /translation="MSAPKSTMRFEQLEQENAMLLREVHVARKASQITAELVVEQFEK
                     MEEVHKELQHRATVERRLRERLLEELEEAEARERELARAREAAEAANRSKSTFLANMS
                     HELRTPLNAIIGYSELLQEEAKDAGLEELSPDLERINSAGKHLLSLINDILDLSKIEA
                     GKIELFLETFDVRAAVSEVMTTIEPLATKNGNQLEVFVADDVGTMHADLVRIRQCLFN
                     LLSNACKFSENGVISLQVRQQVDEQPFLLFDITDTGIGMTSEQLGKLFRPFTQADASS
                     TRKYGGTGLGLSITKRFAEMLGGSIRVESEPGKGSTFSLRIPVHVVKRTMPEEPEPIS
                     SRVEPDSNAVTILVIDDDANVREWMSRSLNNHGYRAVTAANGREGLELAKKLNPAVVV
                     LDVMMPEMDGWAVLTALKAEPSLTDIPVVMAT"
     gene            complement(1271..1750)
                     /locus_tag="PK710_21065"
     CDS             complement(1271..1750)
                     /locus_tag="PK710_21065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOT12282.1"
                     /translation="MSWTVESKPVRCRVLCLGNRYVAEDAFGPQVHDVLQDWGMPDGV
                     ELVDGGIAGLNLLHCFDHVKQVIVVDALKGFGTAGKLLELSPEQAIFEEDQPLGHGTG
                     LPTLLRLMELSCESDIPSVTIVGVEVPADEQSVKETARRVLFMLGSDVGMTIPPEGK"
     gene            complement(1747..3276)
                     /locus_tag="PK710_21070"
     CDS             complement(1747..3276)
                     /locus_tag="PK710_21070"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOT12283.1"
                     /translation="MSVKTIVLHMNRVEGDLEVHVDVADGVVVDAWCKGTMYRGFEKI
                     LVGRGPLDGLVITPRICGICSTSHLLAAARALDDVFDVEPPPDAHRVRNVTLMTETVQ
                     SDIRHTVLMFAADLVNPAHRSDPHFEEAVRRFEPFRGESVVEVIRETKKILEIVAILG
                     GQWPHSSFMVPGGIVSVPSSADLQRCLWLLRSFQSWYERQILGCSLERFAQIESVAEL
                     HAWLDESPRHQNSDFGFLLRAMRSMRLDEIGAGPGNYLSVGSLEMPNDTRIKAVQDSH
                     FLEPAGFAQGTEVEAFEQESIREHVAHSWFMDYPGGRHPFDGETRPYATGAEGSKYSW
                     AKAPRYADRVVETGPLAEAVMSGQSLIRDMLSQGPNAMVRQIARIIRPVRLIPAMYAW
                     LEEARGDGHYYVKPKPLQNGRGFGLCHAPRGSLGHWVEITDGAISHYQIVTPTAWNAS
                     PRDSENRRGAMEEALIGTPVKEVDNPIDVYHVVRSFDPCLVCTVHTLRKPLNREWNGA
                     A"
     gene            complement(3294..>4094)
                     /locus_tag="PK710_21075"
     CDS             complement(3294..>4094)
                     /locus_tag="PK710_21075"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12284.1"
                     /translation="NGTPSQHHALLEAICHGEQKLDILCVEGAIVRGPGGTGMFDTME
                     GVPKKDLFARLAKVAGDVVAVGTCACFGGIGADGEIEATGLQFLKATPGGFLGSSYRS
                     ANGNVVVNLPGCPCHGDVIASTIATIAQKAPLPLTEWNSPVEWYDLLVHQGCVRNEYH
                     EYRIEEREFGERGCLFFHLGCRGPVAYGPCNKILWNRRSSKTRVGLPCVGCTEPDFPQ
                     SHPFFQTPSIVDIPIELPDGVDRAHYLAYKGMAAAAAPDRLKQRKTRV"
BASE COUNT          890 a         1224 c         1060 g          920 t
ORIGIN      
        1 atggtggcca tcacgacagg gatatccgtc aatgacggtt ctgctttgag tgcggtcagc
       61 acggcccaac cgtccatttc gggcatcatg acatcgagca cgacaacggc gggattcaat
      121 tttttggcga gttccagacc ttctcgaccg ttcgcagccg tgaccgcacg ataaccgtgg
      181 ttgttcaacg agcgcgacat ccactcccgc acgttggcat catcatcgat gaccaagatc
      241 gtcaccgcgt tcgagtccgg ctcgactctc gaggaaatgg gctctggctc ttccggcatg
      301 gtccgtttca ccacatgaac cggaatgcgc aaggagaagg tagagccttt ccctggttcg
      361 ctttcgaccc tgatactccc cccaagcatt tcggcgaatc gcttggtaat gctcaaacca
      421 agccccgttc ctccgtactt gcgcgtggat gaggcgtcgg cttgcgtgaa tggccgaaac
      481 aatttgccaa gttgttcgct cgtcatcccg atgcccgtgt cggtgatgtc gaagaggaga
      541 aagggctgct catcgacttg ctggcgaacc tgcaaagaaa tcacaccgtt ttccgagaac
      601 ttacatgcgt tcgacaacaa gttgaacaag cattgccgta tgcgtaccag gtctgcatgc
      661 atcgtcccga catcgtcggc cacgaagact tccaattggt ttccgttttt tgtcgcaagc
      721 ggttcgatcg tcgtcattac ctcggaaacg gccgcgcgaa catcgaaggt ttcgaggaat
      781 aactcgattt tgcccgcttc gatttttgac aaatcaagga tgtcgttgat gagcgatagc
      841 aagtgttttc cagcggaatt gatgcgctcg agatcgggag acaactcctc cagccccgcg
      901 tctttcgctt cttcctgcaa caactcgctg tacccgatga tggcattcag gggcgtgcgc
      961 agttcatggc tcatattcgc caaaaacgtg ctcttcgaac ggtttgccgc ttccgctgcc
     1021 tcacgcgccc gcgctagctc ccgctctcgt gcttcggctt cttccagttc ttcgagcaat
     1081 cgctcacgca accgacgctc caccgtggcg cgatgctgca gttctttatg aacttcctcc
     1141 attttttcga actgctccac cacgagttct gctgtgatct gcgacgcttt tcgtgccaca
     1201 tggacttccc gaagaagcat cgcgttctcc tgttcgagtt gttcgaaccg catcgtgctt
     1261 ttgggcgcgc tcatttcccc tccgggggta tcgtcatccc aacgtcggac ccgagcatga
     1321 aaagcacacg acgagctgtc tctttcacgc tttgctcatc ggcaggcact tcgactccta
     1381 cgatcgttac cgatggaatg tcggattcgc agctcagctc catcaagcgt aacaaagtag
     1441 gcaagcctgt accgtgcccc aagggctggt cttcctcgaa aatggcttgc tctggactca
     1501 actcgagcag ctttcccgcc gtgccaaacc ctttcaacgc atcgacgacg atcacctgtt
     1561 tcacatggtc gaagcaatgc aacaagttca atccagcaat gccgccgtcc accaactcga
     1621 caccgtcggg cataccccag tcttgtaata cgtcatggac ttggggacca aaggcatcct
     1681 ccgccacata acgattgccc aagcacagga cccggcaacg gaccggtttg gattcgacgg
     1741 tccaactcat gcggccccgt tccattcgcg attcaatggc tttcggagtg tgtgtaccgt
     1801 gcagaccaaa caaggatcga acgatcgcac cacgtggtac acatcgatcg gattgtccac
     1861 ctcttttacg ggtgtgccga tgagcgcttc ttccatggcc ccacgacgat tttcgctgtc
     1921 cctcggcgat gcattccacg cggtgggggt gacgatctga taatgagaaa tagccccatc
     1981 ggtaatctct acccaatgtc ccagggaacc gcgaggggca tgacacaagc caaaacctcg
     2041 accattctga agtggtttgg gttttacgta ataatgtccg tcgccccggg cttcttcgag
     2101 ccaggcatac atcgcgggaa tcaagcgaac ggggcgaatg atgcgagcga tttggcggac
     2161 catggcgttg ggtccctggc tcagcatgtc tcgaatcaag ctttggccgc tcatcaccgc
     2221 ttcggcaagc gggcctgtct ccaccacacg gtctgcatag cggggagctt tggcccaaga
     2281 atatttcgat ccttcggctc ccgtcgcata aggccgcgtc tcaccgtcga aaggatgtcg
     2341 tcctcccggg taatccatga accatgaatg ggcgacgtgc tcccggatgc tttcttgttc
     2401 gaacgcttcg acttcggtcc cttgtgcaaa accggcgggc tcgaggaaat gggagtcttg
     2461 gactgcttta atccgcgtat cgttcggcat ttcgagggaa ccgacggata aatagtttcc
     2521 gggaccggct ccgatctcgt cgagccgcat cgaacgcatg gcccgcagca aaaaaccgaa
     2581 atcactgttc tgatgccgcg gggactcgtc gagccatgca tgtagttcgg ccacactttc
     2641 gatttgggca aatcgttcaa gactgcaacc cagaatttgt cgctcgtacc acgattgaaa
     2701 cgagcgaagc agccacaggc atcgttgtag gtccgcagac gatgggactg atacgattcc
     2761 gccaggaacc atgaaagagc tatgcggcca ttggcctccc aagatggcga cgatctcgag
     2821 aatttttttg gtttcgcgaa tgacctccac caccgattcg ccccggaacg gttcgaaacg
     2881 tcgaacggcc tcttcgaaat ggggatccga acgatgtgcc ggattgacaa gatcggctgc
     2941 aaacatcagc acggtatggc gaatgtcgct ctgcaccgtt tccgtcatca atgtcacgtt
     3001 gcggacacga tgggcatccg gtgggggttc gacatcaaaa acgtcatcaa gcgctcttgc
     3061 cgcggcaagc aaatggctgg tgctacaaat cccgcaaatt cttggcgtaa tgaccaaccc
     3121 gtccaacggc ccccttccca cgaggatttt ctcaaacccg cgatacatcg tccctttgca
     3181 ccaggcatcg acgaccaccc catcagcgac atccacatgc acctccaagt caccctcgac
     3241 gcggttcatg tgcaagacga tggtcttaac ggacatgggg gttcagtgct cctttataca
     3301 cgtgtttttc gctgcttgag ccgatcaggg gccgccgccg ccgccatgcc cttgtaggca
     3361 aggtaatgag cgcgatcgac gccatcgggc aattcgatgg gaatgtcgac aatggaaggc
     3421 gtttgaaaaa atggatggga ttggggaaag tccggttcgg tgcagcccac acacggcaat
     3481 cccacgcggg ttttcgaact gcgacgattc cagaggattt tgttgcaggg gccgtaggcg
     3541 acaggccctc ggcatccaag gtgaaagaac aggcagccac gctcgccgaa ctcacgctcc
     3601 tcgatgcggt attcatggta ttcgttacga acgcagcctt gatgcaccag aaggtcgtac
     3661 cattcgactg gggaattcca ttcggtcaag ggtaggggag ctttctgtgc aatggttgcg
     3721 atggtgcttg caatgacatc cccatgacac ggacaacccg gcaaattcac tacgacattg
     3781 ccattggccg agcgatacga cgatccaagg aatccccctg gcgttgcttt caaaaactga
     3841 agacccgtgg cctcgatttc gccatcggca ccaattcccc cgaaacaggc gcaggtgccc
     3901 actgccacga catctccagc cactttcgcc aatcgcgcaa acaaatcctt tttgggaacc
     3961 ccttccatcg tgtcgaacat tcccgtccca ccaggaccgc gcacgattgc cccctccaca
     4021 caaaggatat cgagcttctg ctcgccgtga cagatcgctt cgagcaaagc gtgatgttgc
     4081 gacggggtcc cgtt
//
LOCUS       DAOSOR010000531         4088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_21982_length_4088_cov_4.144557, whole genome shotgun sequence.
ACCESSION   DAOSOR010000531 DAOSOR010000000
VERSION     DAOSOR010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21982_length_4088_cov_4.144557"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PK710_21080"
     CDS             <1..555
                     /locus_tag="PK710_21080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12285.1"
                     /translation="DHRADLWAVGVMFYEMVTGQEAFDAPTDFAKLTAVLTQDPRPIS
                     QVAPHLVGWDDFFRRAVARDVAARFSSADDMARVLLATARALAGAAPLPTAEFRSIDV
                     EKEKVSSNPPVGREGTHVSAQLPSGPQSPSIAPSVQVIESGHHPPVQPRTGVKPVAVV
                     LIGLLCLMMGFAAGAATMWAALRL"
     gene            764..922
                     /locus_tag="PK710_21085"
     CDS             764..922
                     /locus_tag="PK710_21085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12286.1"
                     /translation="MAFSIVTEISLCILKGRDFFTLALTPVVAKKDIDHAPSEPSLAE
                     VSCTYARK"
     gene            1149..1700
                     /locus_tag="PK710_21090"
     CDS             1149..1700
                     /locus_tag="PK710_21090"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT12287.1"
                     /translation="MRKRFIPVIFTIAALVPIAACSKTPEPVTPSTPTEEAPPPQVSP
                     VQQAAIDDAEKETGGTGLGIDDEILRLCPAVRPPKFGYDSARVKDEFRNTLVALAQCM
                     NDGALKGRSLLMVGHADPRGEEDYNMALGGRRAESVRGAMESLGVDKGRMDVSSRGEL
                     EATGVDEESWAKDRRVDIKLKAN"
     gene            1700..2491
                     /locus_tag="PK710_21095"
     CDS             1700..2491
                     /locus_tag="PK710_21095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12288.1"
                     /translation="MSRVLARIARFIRSFGFTVLSVGLHVALVVLCVLFSTPADSMAA
                     DREWIEVDMPPALGQPHGKDEGKDLGSHIPKAPKMPEPEPFEAPPPVPPPDPEAIPTT
                     VSSAQPAADGGPAAEADAGDAGSFETGAVGEGEGDPTTPVGSENVPPAFGSPDGVPGG
                     QGTGPRKISPQYMAILAAWARARFPTHGHGLGPPPEGTRPCARVSIQIAPDRQVSGSS
                     MTSSGQAVWDDKVRAALPAFSGASVPADPEGGPPPPSIGLNVCWD"
     gene            2625..4040
                     /locus_tag="PK710_21100"
     CDS             2625..4040
                     /locus_tag="PK710_21100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12289.1"
                     /translation="MNTSTFYRRCFRVGLLGAAVCFTVPAFAQLTSRDMPGTDGAVAG
                     SQVEEPLIEISVTSEQMPVLIVAPATTADAPLAEQIAAEIQLTGLFNVRTTVSMRLGA
                     GLPERSKWPALSTAAVVVGRLQRPSGFPIVISDTYHAAESDVYRRRELVLYPGSEPMM
                     VSTMADAILEDVLGIRSHMSGKLVLSDASTRGERSVRVLAPNGSRGRRVSGFGTLARG
                     ADFDRDMRTWYATEDPSGKLQLFQEGQTTPVALGIPGYVQSIGFSPHGQFVALSMGEG
                     DRVRTWKGPSTDQLRMMPLPEEQTALSPSIDDQGRVVHVVGPVNGPFSVMVDEKRVTP
                     PGVWAAMPSFCSTLVEDRVVYMVRAGRSWHIRITSLTNGSSRTVATNAMSPACSPDGR
                     TVAFFSNGLYGKGPGVYLTADFGGQARKVWDGQAAGLRWRGGESLPSKTIVTIASDEP
                     ETTEPSPADPPAKGAVPGSDD"
BASE COUNT          766 a         1236 c         1172 g          914 t
ORIGIN      
        1 gaccatcgcg ccgacctttg ggctgtcggt gtgatgttct acgaaatggt gacaggacaa
       61 gaggctttcg acgcccccac cgatttcgca aaacttaccg ccgtgctcac ccaagacccg
      121 cgacccatct ctcaagtcgc tccccacctc gtcggatggg acgacttctt ccgccgtgcc
      181 gtggctcgag atgtagcggc ccgcttcagt tccgccgacg acatggcgcg tgtgttgctc
      241 gccacggcca gagcgcttgc gggagcagct cctttgccca ccgcggagtt ccgatccatc
      301 gacgtcgaaa aagaaaaagt atccagcaat cctccggtcg gtcgagaagg cacccatgtc
      361 tccgcccaac tcccttccgg tccgcaaagc ccctcgattg cgccaagcgt tcaggtcatc
      421 gagtccggtc atcatccgcc cgtgcagccc cgaaccggcg taaaaccagt ggccgtcgta
      481 ctcataggcc tgctgtgtct gatgatgggg ttcgctgctg gcgccgcaac catgtgggct
      541 gcgttgcgcc tgtgattggc ctttgcgttt cgtccggctc gctgcttcta tccccttgct
      601 cctgacccac cctccgcaaa acgaccaaaa tcttcactcc cacgcccttc cccctcatcc
      661 tttgtaaata tttgtaatat ttgaattagt tgattttgat gcaatttcga aaacttctgt
      721 tccgttctgt ggataagtgt tcgatatttc tgagtatgat gccgtggcct tctcgattgt
      781 tacggaaatt tccctttgca ttctcaaggg tcgtgacttt ttcaccctcg cgctcacccc
      841 tgtcgtcgca aaaaaagaca tcgaccatgc cccttcggaa ccgtctttgg cggaggtttc
      901 ttgcacttat gcaagaaagt gaggtatggg cgcctgggat gggtgtgtcc aggaatcaac
      961 cacccagatg gttggttgtt agccgtatgg gctgacccca gccaaactcc cgaacatccc
     1021 atggttttac gacgtaccca acacggtggt tgcccttcga ttcgcgatga cagggctgtc
     1081 atttccctca accatgggat ttttgaacat tattggagca atgtcacgaa tcagttttgg
     1141 aggatcccat gaggaagcgt ttcatccccg tcattttcac gattgccgct cttgtcccca
     1201 tcgccgcttg cagcaaaacc cccgagcccg tcactccttc gactccaacc gaagaagcac
     1261 cgccgccaca agtctctccg gtgcagcaag cggccattga cgatgccgaa aaagaaaccg
     1321 gcggtactgg gcttggtatc gacgacgaaa tccttcggct ttgccctgcg gttcggcctc
     1381 cgaaattcgg atacgattcg gcccgggtga aagatgagtt tcgcaacacc ttggtggcgc
     1441 tggcgcaatg catgaacgac ggtgccttga aaggacgcag tttgctgatg gttggacatg
     1501 cggacccgag gggcgaggag gattacaaca tggcgctcgg cggcagacgt gccgaatccg
     1561 tgcgcggcgc tatggaatca ctgggagtcg acaaagggcg catggacgta tcctcccgcg
     1621 gtgaactcga agccaccggt gtcgacgaag aaagctgggc caaagaccgt cgcgtcgata
     1681 tcaaactcaa ggcgaactga tgagtcgggt gttggcgagg atagcacgat ttatccgttc
     1741 attcggattt accgtgcttt ccgtggggtt gcatgtggcc ctggtggtgt tgtgtgtgct
     1801 tttttccaca cccgccgatt cgatggccgc ggatcgggaa tggatcgagg tggatatgcc
     1861 tcccgccctt gggcagcctc acggcaagga tgagggcaaa gacctcggtt cgcatattcc
     1921 caaagccccg aaaatgccag aaccggagcc tttcgaggcg cctccgcccg tgcctcctcc
     1981 tgaccccgaa gccattccaa cgaccgtttc ttccgcgcag cccgctgccg atgggggacc
     2041 ggcggcggaa gccgatgcgg gggacgctgg atctttcgaa actggcgcgg ttggggaagg
     2101 ggaaggggac cccaccacgc cggtgggcag cgaaaacgtt ccgccggctt tcggctcccc
     2161 agatggtgtt ccagggggtc agggaaccgg ccctcgaaag atttcgccgc aatatatggc
     2221 aattttggcc gcctgggcgc gcgctcgttt cccaacccac ggccatgggc tcggcccgcc
     2281 tcccgaggga acgcgtccct gtgcccgggt cagtatccag attgcccccg accgacaagt
     2341 atcgggctcg tcgatgactt ccagcggaca ggcggtatgg gacgacaagg tccgggctgc
     2401 cctgccggct ttcagtggtg cttcggttcc cgcggacccg gaaggaggac ccccgccgcc
     2461 ttccatcggg ctcaatgtct gttgggattg agtcaatcga cgtgggtgtt ttgaacgtca
     2521 ttgccccatg cggtcggttt gacggctctt tcgtgaagac ctccgatgga caatgaatac
     2581 ggccgctgct cataaggtct tactgcctga tttggactcc tgtcatgaat acatccactt
     2641 tttatcgtcg ttgttttcgt gttggcctgc tcggcgccgc cgtctgtttc accgttcccg
     2701 cttttgcaca actgacctca cgggatatgc caggaaccga cggggcggtc gcgggttctc
     2761 aagtcgaaga gccgctcatc gaaatctcgg tcacctccga acaaatgccg gttctcatcg
     2821 tggcccctgc cacgacggcg gatgcgccct tggccgaaca aatcgctgct gaaatccaac
     2881 tgacggggct attcaacgtg cgcacgaccg tctcgatgcg gctcggcgcg ggccttccgg
     2941 agcgaagcaa atggcctgcc ttatccactg cggcggtcgt ggtgggcagg cttcagcggc
     3001 cctccggttt tcccatcgtc atctccgata cctaccatgc cgcggaatcc gacgtctatc
     3061 ggcgacgcga actcgtttta tatcctggca gcgagccgat gatggtttcg accatggccg
     3121 atgccatcct cgaagatgtg cttggcatcc gctcgcacat gtccggcaaa ctcgttctgt
     3181 cggatgcctc cacccgcgga gagcgttcgg tgcgtgtgct ggctcccaat ggctctcggg
     3241 ggcgtcgggt cagcggtttc ggcacgttgg cccggggtgc ggacttcgac cgcgacatgc
     3301 ggacttggta tgcgacggaa gacccttccg gaaaattgca gctttttcaa gaagggcaaa
     3361 cgactcctgt tgctcttgga attcccggct acgtgcaaag catcggcttt tctccccacg
     3421 gacagttcgt tgccctatcc atgggggagg gcgaccgcgt gcggacctgg aaaggtccct
     3481 ccaccgacca acttcgcatg atgcccttgc cggaagagca aaccgcgttg tcgccatcga
     3541 ttgacgatca agggcgggtg gttcacgtgg tgggacctgt caatggtccg ttttccgtca
     3601 tggtggatga aaaaagggta acgcctcccg gcgtttgggc cgcaatgcct tctttttgtt
     3661 ccacactcgt cgaggaccgc gtggtgtata tggttcgggc gggacgaagc tggcatattc
     3721 gcatcaccag cttgaccaac ggttcttcac ggaccgtggc caccaatgcc atgtcccctg
     3781 cttgtagtcc ggatggcaga acggttgctt tcttctccaa cggcctttac ggcaaaggtc
     3841 caggcgttta tctgacggcg gactttggag ggcaggctcg taaggtgtgg gacgggcaag
     3901 ccgccgggct tcgttggcgt gggggcgaaa gtcttccatc caaaaccatc gtgacgatcg
     3961 cctcggacga gccagaaacg accgaacctt ctcctgccga ccctccggcc aaaggggccg
     4021 tccccggttc cgacgactag tactacagat cctttcttcg ttccacatta ggaccggggc
     4081 gtaagaac
//
LOCUS       DAOSOR010000532         4080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_22034_length_4080_cov_4.961491, whole genome shotgun sequence.
ACCESSION   DAOSOR010000532 DAOSOR010000000
VERSION     DAOSOR010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22034_length_4080_cov_4.961491"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2281
                     /locus_tag="PK710_21105"
     CDS             <1..2281
                     /locus_tag="PK710_21105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12290.1"
                     /translation="ARRGDLLDELSAVSNAQPSHRAGWLMEAAEIAMLKGDSHRALDR
                     ARRAAALVPSDTEIQLRVVFMTYRLEGLSDRRLVSDMLAQLRRIEAGLSPEDIDLHGF
                     LTAECIEVWQGPDAAMTALQQQIQRTGTGPLLALALADRYSIMGDPHLALPLFDMALG
                     AEDLRGVRTRAQVAFCAARAALRAGGAQLAEPYVALLEREPGSEPLVQRLRVEMTELV
                     PTTDEVRRQLNQVARASRGLSRAQALWQLARISAARNTRESNAEAEGYYGEAIAAASS
                     DATLRKEMEVERDAFRSRLTPSLLPPPARISTVPPAAQVPKVENEPSVDTWVSAIAPS
                     PLSPPVSPSPADTAPVVADTAPVIADAALPPADATPSIETAAPTRPIVDPNRENKLLQ
                     ELAKGNMQAGDELASWISDNPTRSSEVVAIRRRQVVLEPHSVRHLELLRDAARADRNA
                     DYAAALHHVVCVLTGQTAPPPPNLQPSSHDPERLLTMVGRGVYGAAADVLGEVWRSAS
                     HLFVREPAAYGLTGIERISLISPSPVGRAYAKAAGILGLGKVPVFQRRTEGAMALGVA
                     ALSPMAVVVSGEIVDGEPLEYRMAAMMMGTTPPNAMLFGLPPTAVRQLMRALQAAFGP
                     PSAARERASESAVLAAELWRALPGSAQRRFAQLYRDSEPFSYEDAWARALQVTRRAGL
                     LVVGNLAVALKDALSDPGMQQAIDTGAPDAYRHLCRVSVSAADLVRFAASCEYADVRW
                     HDTRHGSGAYWSSTTGSRP"
     gene            2283..2915
                     /locus_tag="PK710_21110"
     CDS             2283..2915
                     /locus_tag="PK710_21110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12291.1"
                     /translation="MTRLSGRDSHDWHLLNLPGVARFSLLLCCVTFLGNPSCLVTSTT
                     EFAEPVLSPPFVDANTALATPQGGAGVPITRLLRVDADTREVTLSTDVRSDDAGRPLW
                     SRAFINYKLGDRPFDEIFGGEVIPPGTFDEVRHIGASFDPGRLNDGCYQVSLVITHEF
                     DAKELIPIRQDDTAILVWWMIKGDPALISLAQCPGVPPSSTLDAGIEGGS"
     gene            2917..>4080
                     /locus_tag="PK710_21115"
     CDS             2917..>4080
                     /locus_tag="PK710_21115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOT12292.1"
                     /translation="MRTCPLAFFLTVCLLSCSVDQMPDHSPTTRVVNTCKIDSDCRAS
                     GRQGMCGSGMCVASSGRLTQGILEIVPPASAFFGAGDSFLIPIDDISHGRWNEDLALP
                     RYANVEGILTTTPSVLGDPPSESCSQAFDTTTQSLRVHVDFSRSDASQGLPGYTVAAN
                     AERVEGGTGFRFAASVPSGKYDVYATVIGDCEADFPPLFAPKKTLDPGNVMMELHVGA
                     LSTLTGTVTPPVSSTGEQISLTGWTVSLVEPEQGKVISTMRKLNDSNPTNFEIRYQPL
                     GETPPILQIVPPKDLIAPTITWDLSVLDLDGDGQVQPSISALDLATVQVNASVVTHEV
                     EAVAGATVRLRSTALMGASAGLNARYETSATTDAEGMFSLSLLPGTYQVVVTPP"
BASE COUNT          755 a         1131 c         1297 g          897 t
ORIGIN      
        1 ggctcgtcgt ggggacttgc tcgatgagct atcggcggtg tcgaacgcgc agccttcgca
       61 tcgagctggc tggttgatgg aagcggcgga gattgcgatg ctcaaggggg atagccatcg
      121 tgcgctcgac cgggcgcgac gcgcggcggc gctggttcct tcggacaccg agatccagct
      181 acgcgtggtg ttcatgacgt accgtctcga ggggttgtcg gaccgacgac tcgtgtcgga
      241 tatgctcgcc cagcttcgtc gtatcgaagc gggcctaagc ccggaagata ttgatttaca
      301 tgggtttctt accgccgaat gcattgaagt ttggcaagga ccggacgctg cgatgacggc
      361 gttacaacag cagattcagc gtacgggcac aggtcctttg cttgcgttgg cattggcgga
      421 tcgatattcg atcatgggtg atcctcattt ggcgctaccg ctcttcgaca tggcgttggg
      481 cgcggaggat ttgcgcgggg ttcgaacgcg ggctcaagtg gctttctgtg cggcgcgagc
      541 ggcgttgcgg gcgggaggcg cgcaacttgc cgagccttac gttgcgctgt tggagcgcga
      601 accgggctcc gagcctttgg tgcagcgatt gcgcgttgaa atgaccgaac tggttcccac
      661 gacagacgag gttcgacgtc agctcaatca ggtggctcgc gcgtcgcggg ggttgtctcg
      721 cgcgcaggcg ctgtggcagc ttgcgaggat atcggcggcg agaaacacgc gcgaatcgaa
      781 tgcggaagcg gaaggatatt atggggaagc catcgccgcg gcatcgtcgg atgccacgtt
      841 gcgaaaggaa atggaagtcg agcgcgatgc gtttcggagt cggttgacgc cgagtctttt
      901 acctccaccg gctcgtattt ccacggttcc gccggctgcc caggtgccaa aggtggaaaa
      961 cgagcccagc gttgacacat gggtcagcgc gatagcgcct tcgccattgt ccccacctgt
     1021 ctcgccatcg cccgcggata ctgctccagt tgttgcggat actgccccag ttattgcgga
     1081 tgcggcgctg ccgcccgcgg atgccacgcc atcgatcgag acggcggcac ccacgcgtcc
     1141 catcgtggac ccgaatcgag aaaacaagct tctacaagaa ctcgccaagg gcaacatgca
     1201 agcgggtgat gagctagcct cgtggatttc cgataatccc actcgctcga gcgaagtcgt
     1261 ggcgatacga cgtcggcaag tggtgcttga accgcatagt gtgcggcatc tggaattgct
     1321 aagagacgct gcccgggctg atagaaacgc ggattatgcg gcggccctcc accatgtcgt
     1381 ttgtgtgttg accgggcaga ccgcgccacc gcctccgaat cttcagcctt cgtcgcacga
     1441 tccggagcgt ctgctcacca tggtaggtcg aggagtgtat ggggccgcgg cggatgtgct
     1501 tggagaggtt tggaggagtg catcacacct tttcgttcga gaacccgccg cctatggatt
     1561 gactggtatc gagcgaatat cgctgatttc tccttcgccc gtgggaagag cttacgccaa
     1621 agcggccggg atccttggat tgggcaaggt gcccgtattt cagcgaagaa cggaaggagc
     1681 gatggctctg ggtgtggccg ccctatcgcc gatggcagtg gtcgtcagtg gtgagattgt
     1741 cgatggggag ccattggagt atcgaatggc ggcgatgatg atgggaacga caccgccgaa
     1801 tgcgatgtta ttcgggcttc ctcccacggc ggtgcggcaa ctcatgcgtg cgttacaagc
     1861 ggcctttggt cctccaagcg cggcgcggga gcgtgcgagt gaatccgccg tccttgccgc
     1921 agagttgtgg cgtgcgcttc cgggctctgc ccagcgtcgc tttgcccagc tttatcgaga
     1981 ttccgaacct ttctcgtatg aggatgcatg ggctcgggct ttgcaagtca cgcgacgcgc
     2041 ggggttgttg gtggtgggga acctggcagt ggcgttgaag gatgcgcttt cggacccagg
     2101 gatgcagcag gccatcgaca ccggcgcgcc agatgcatac cgtcaccttt gtcgtgtatc
     2161 cgtatccgct gctgatctcg ttcgtttcgc ggctagctgc gaatatgccg acgtgcggtg
     2221 gcacgacacc cggcatggtt ccggggcgta ttggagttcg acgacaggat cgaggccctg
     2281 atatgacacg gttgtcaggg cgcgactccc atgattggca tctgcttaac ttgccgggcg
     2341 tggcacgttt ctcgctcctg ctctgttgtg tgaccttcct cggtaatcca agctgccttg
     2401 ttacctcgac cacggaattc gcagaacctg tcctctcccc tcctttcgtc gatgccaaca
     2461 ccgccttggc aacgccgcaa gggggagcgg gagtgcccat cacacgattg cttcgcgtcg
     2521 atgccgacac gcgagaagta acactatcga ccgatgtgcg ttccgacgac gcgggccgtc
     2581 cgctgtggag ccgagcattc atcaattaca aactggggga tcgtcctttc gacgaaatat
     2641 ttggtggcga ggtcattcct cccggcacct ttgatgaagt tcggcatatc ggcgccagtt
     2701 tcgatcccgg acgtctcaac gatggttgct accaggtgtc tctcgtcatc acccacgaat
     2761 tcgatgcgaa agaactgatt ccgattcgtc aggatgacac cgcaatcctg gtatggtgga
     2821 tgatcaaggg tgacccggcg ttgataagtc tcgcgcaatg cccgggcgtg cccccgtcat
     2881 ccacgctgga cgccggcatc gagggaggtt cgtgatatgc gaacctgccc cctcgctttt
     2941 tttctcaccg tctgcctgct gtcgtgctcc gtcgaccaaa tgcccgatca ttcacccacc
     3001 acccgcgtcg tcaacacctg caaaatcgac tcggattgta gagcctcggg gcgtcaggga
     3061 atgtgtggct cgggaatgtg cgtcgcctcg tcagggcggc tgacccaagg aattctggaa
     3121 attgtgcccc cggcttccgc atttttcggt gcgggagact ctttcctcat ccccatcgac
     3181 gacatctccc acgggcgctg gaatgaggac ttggccctac cgcgctatgc caatgtcgaa
     3241 gggattctga ccaccacgcc gagcgtgttg ggggatcctc cttccgaatc ctgttcgcaa
     3301 gcattcgata caacgacgca atcgctgcgt gttcatgtgg acttctcccg tagcgatgcc
     3361 tcgcaaggat tgcctggata cacggttgcg gccaatgccg agcgcgtgga aggcggcacg
     3421 ggttttcggt ttgcggcatc ggttccctcg gggaagtatg atgtctacgc gacggtgatt
     3481 ggggattgtg aagcggattt ccctccgcta tttgctccga aaaaaacgtt ggatcccgga
     3541 aatgtgatga tggagctgca tgtgggggct ctttccacgc taacgggaac ggttacgcca
     3601 cccgtatcgt cgacgggcga gcaaatatcg ttgacggggt ggacggtatc gttggtcgag
     3661 cctgagcaag gcaaggtcat ttccacaatg cgaaagctca acgattcgaa ccccacgaac
     3721 ttcgaaatac ggtaccagcc tttgggcgag acgcccccga tattgcagat agtcccgccc
     3781 aaagacttga ttgcgccgac gataacgtgg gatttatcgg tattggatct ggatggtgat
     3841 ggtcaagttc aaccgagcat atcggcgttg gatctggcta ctgtgcaagt caacgcgagt
     3901 gtggtgaccc atgaggtgga ggcggtggcg ggggcgacgg tacgtcttcg cagtacggct
     3961 cttatgggag catcggcggg gttgaatgct cgttacgaga catccgcgac aacggatgcg
     4021 gaaggcatgt tttcgttgtc gctgttgccg ggaacgtatc aggtcgtggt gacacccccg
//
LOCUS       DAOSOR010000533         4079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_22041_length_4079_cov_4.232107, whole genome shotgun sequence.
ACCESSION   DAOSOR010000533 DAOSOR010000000
VERSION     DAOSOR010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22041_length_4079_cov_4.232107"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2142)
                     /locus_tag="PK710_21120"
     CDS             complement(<1..2142)
                     /locus_tag="PK710_21120"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOT12293.1"
                     /translation="MVEHVGVTSTQTGKRARRVREFGSERGLSFERRFSCSDTPPLQS
                     ISWEERSSSITDPDGTVVFQLDNVTVPSSWSQLATDIVVSKYFRKSGLYGQKDRGETS
                     VAQVVERLAHTIREAGERFGYFSTASDAAIFQDELTYLLVNQYGAFNSPVWFNCGLWH
                     RYQIVGSGGNYAWDPGTDSIVETNNAYERPQCSACFIQSVDDDLMSIYELVKTEARLF
                     KYGSGTGTNFSPIRARQEKLSGGGTSSGLMSFLEVFDRAAGATKSGGTTRRAAKMVTL
                     DMDHPEIVDFIDWKLREERKAKALIAQGYEADFNGEAYHTVSGQNSNNSLRITDDFMR
                     ACTVGGEWKTIARTTGAVVERLNAKVLWNKVAEAAWACADPGVQYDTTINRWHTCPNS
                     GRINASNPCSEYMFLDDSACNLASINLVKFRRQDGSFDVAAYRHAIRVFFLAQDILVD
                     FSSYPTERIARNSHRYRPIGLGYANLGTLLMIEGLPYDSDQARAFAAALTGIMTGEAY
                     ATSAEIARTKGTFEAFAINREPMLHVMGMHRDAAYGIQREHCPEALWKAACDVWDEAV
                     AMGEQVGYRNAQATVLAPTGTIGLLMDCDTTGIEPDFALVKFKKLAGRGYFKIVNQAV
                     PGALRKLGYSEAQVQDIITYISGTGTLTGSPHINRASLLQRGLDEQDLRNIEGKLSGV
                     FDISQAFAPWVLGETTMARLKATAAASKPGFS"
     gene            complement(2801..3466)
                     /gene="ung"
                     /locus_tag="PK710_21125"
     CDS             complement(2801..3466)
                     /gene="ung"
                     /locus_tag="PK710_21125"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOT12294.1"
                     /translation="MRSLSPSWQAVVGDEFDKPYFNKLVAFLRQERSCKTVFPSDDEV
                     FRALDLVPYHDVRVLLLGQDPYHNEGQANGLCFSVRPPTRPPPSLRNIFRELESDLGI
                     PAPKHGDLTAWAHQGVLLLNTVLTVEAHKAASHAGKGWERFTDAVIEALNRRNVPLVF
                     CFWGAHARKKVCLIDTARHPVVQAAHPSPLSQKKFLGSRPFSTINAALVSKGLTAVDW
                     RIP"
     gene            complement(3508..>4079)
                     /locus_tag="PK710_21130"
     CDS             complement(3508..>4079)
                     /locus_tag="PK710_21130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12295.1"
                     /translation="RFCWDRALADHRFFPCVIAAPSSTSACKLPQALWGDFDLDHFLA
                     YTLRATRDQVRIDLSRVVVVGHSGAGCNPRGGLVTAMQSTLPLVGGLVVDVCMDRLDA
                     QPLARARSDMDVVITYQRKWRRDFSEFSALFQEQSQGAQGLRLVQELPILSAQPHTDI
                     LLESLFTWLPRWLPPTEVHSPVAADSKER"
BASE COUNT          909 a         1164 c         1135 g          871 t
ORIGIN      
        1 ggaaaagcct ggtttcgacg cggcagcggt tgccttgagc cgcgccatgg ttgtttcgcc
       61 gagcacccag ggggcaaacg cctgcgaaat atcgaaaacg ccagaaagct tcccttcgat
      121 gttgcgaagg tcttgttcgt cgagcccccg ctgcagcagg ctcgctcggt tgatatgggg
      181 tgagccggta agagtccccg ttccagatat ataagtgatg atgtcctgga cttgggcctc
      241 ggaatagccg agcttgcgta acgcgcctgg gaccgcctgg ttgacgattt tgaagtagcc
      301 tctgcctgcc agcttcttga acttgactag ggcaaagtcc ggctcgatgc ccgtcgtatc
      361 gcaatccatg agcaatccaa tggtgcccgt aggcgcaaga accgtcgctt gcgcattgcg
      421 atacccaacc tgctcgccca tcgccaccgc ctcatcccaa acgtcgcagg ctgccttcca
      481 cagagcttcc gggcagtgct cccgctggat gccatacgcc gcatcccgat gcatccccat
      541 cacatgaagc atcggctccc ggttgatggc aaacgcttcg aaggtgcctt tggttcgcgc
      601 aatctctgcc gatgtcgcat acgcttcgcc cgtcatgatg ccggtcaggg cagccgcgaa
      661 cgctcgtgcc tggtccgagt cgtagggaag cccctcgatc atgagcaggg tgcccaaatt
      721 ggcatagccc aacccaatgg gacgatatcg atgggagttt cgcgcgattc gttccgtggg
      781 atacgaagaa aaatcgacga ggatgtcttg tgcgagaaaa aacacgcgga tggcgtgccg
      841 ataagcggca acgtcaaagg aaccgtcttg ccgccgaaac ttcacgaggt tgatagacgc
      901 aagattacaa gcggaatcgt cgaggaacat gtattcggaa cacggattcg acgcgttgat
      961 tcgaccgcta ttggggcagg tatgccaccg attgatggtc gtatcgtatt gtacgcctgg
     1021 atccgcgcag gcccacgctg cctcggcaac cttattccag agtactttgg cgttcagacg
     1081 ttcgaccacg gcgccggtgg ttcgggcgat ggttttccat tctccgccca cggtgcaagc
     1141 tcgcatgaag tcgtcggtga tgcgcaggga attgttggag ttttgtccgc tgacggtatg
     1201 gtatgcctcc ccgttgaaat ccgcttcgta tccttgagcg atgagggctt tggccttgcg
     1261 ctcttctcga agcttccagt cgataaagtc aacgatttca gggtgatcca tgtcaagggt
     1321 gaccattttg gccgcgcgac gcgtggtccc acccgacttg gtggcgcctg ccgcccggtc
     1381 gaaaacctcg aggaaactca tcaatccgga actggtcccg ccaccggata gcttctcttg
     1441 acgagctcga atcgggctga aattcgtccc agtcccggaa ccatacttga acaaacgcgc
     1501 ctcggttttc accagctcgt agatgctcat caggtcatcg tcgacactct ggatgaagca
     1561 ggccgagcat tggggacgct cataggcgtt gttcgtttcc acgatgctat ccgtgccagg
     1621 atcccatgcg taattgcccc cgcttccgac gatctgataa cggtgccata acccacaatt
     1681 gaaccaaacc ggtgagttga acgcgccgta ttggttgacg agcagatagg tcaattcatc
     1741 ctgaaatatg gcggcatccg atgccgtgga aaaataaccg aatcgttccc ccgcttctcg
     1801 aatggtatgc gccaaacgtt cgaccacctg agcaacactc gtttctcctc gatccttttg
     1861 accataaagc ccagattttc tgaaatattt ggatacgacg atgtctgttg caagctggga
     1921 ccaagaggaa ggaacggtca cgttatccaa ctggaaaacg acggttccat ccggatcggt
     1981 gatgctcgaa gagcgctctt cccaagaaat ggactgcaaa ggtggagtgt cggagcaaga
     2041 aaagcggcgt tcgaaggata atccgcgctc agaaccgaat tcccgcactc gtcgagcacg
     2101 ctttccggtc tgcgtagacg taactccaac gtgttcgacc atggccgaat gtgacatgct
     2161 ttcgcctccc agcgtggcaa cacaaaccac cacccaagac agaaaaaaga acgacgactc
     2221 tttccccgcc ttatcggttt cgacttctat aagaatgcta gcctgttcag aacatccctt
     2281 tggcccctca cccacgcgca ctcatttcac taaactcgcg tacaggctcc gatgaccgaa
     2341 gaaccccgtg ataccctacc gcttgtgccc cttcaagcac aaaccacgag atgtggtaac
     2401 ttgccgttag aacaaccact tacgccaaat cctcgccccg ctgaacaccc gatgggctga
     2461 acgccagacc agaacgccac ctgtcaagaa tttaatcgat ttgccggtga agcatcttcg
     2521 gcgattgttt ttccataata atatccatgc cttagaggct gagggcgatt gcgcttgctt
     2581 ccttcactat ccacattccc ccctttggat accgcccatc gaccatccgt tttcggactc
     2641 ctgtcgcgcg cgtcgtcgtg tcatgccctg ggacgagctt ttcgaaaagc gcgtttcgta
     2701 ccttgttcgc agacgatgta gggggggtat gtcgggtttt gcgttggacc tggaagcgct
     2761 tcggtgggcc ttttgattcg catgtcgtcg aggcgtccag ctagggaatg cgccagtcca
     2821 cggcagtcag tcccttggaa acaagggccg cattgatggt cgaaaatggc cgagacccca
     2881 aaaatttctt ttgggatagg ggagagggat gagccgcttg cacgaccggg tggcgggccg
     2941 tatcgataag gcagactttt tttcttgcgt gcgcacccca aaaacaaaat acgagcggga
     3001 catttcgccg gttcaacgct tcgatgaccg catccgtaaa acgctcccac cccttgcccg
     3061 catgggaagc cgccttatgc gcttccaccg tcagaaccgt gttgagaagc aagacgccct
     3121 ggtgtgccca ggcggtcaga tcgccatgct tgggagcagg tatgccgaga tcgctttcca
     3181 gctctcgaaa aatattgcgc agcgaaggtg gcggccttgt gggagggcgc accgaaaaac
     3241 acaacccatt ggcttgtcct tcgttgtggt acgggtcctg gccgagcaac aacacacgaa
     3301 cgtcgtggta gggcaccagg tccaacgcgc ggaacacctc atcatcgctg ggaaatacgg
     3361 ttttgcagga gcgctcctga cgaaggaacg ccacgagctt attgaagtat ggcttgtcga
     3421 actcatcgcc aacgacggct tgccaggagg gagacagact gcgcatggca tggatcccat
     3481 agctgatatt cggcgttggg gcgcgtatta ccgttctttc gaatcggcag cgacagggga
     3541 atgcacttcg gtgggcggca accaccgcgg caaccacgtg aaaagtgact ccagcagaat
     3601 gtccgtatgc ggttgagctg agaggatggg cagttcttgc accaggcgca agccttgtgc
     3661 tccttggctt tgttcctgaa atagagccga gaattcggaa aaatctcgac gccattttcg
     3721 ttggtaggtg atcaccacgt ccatgtcgga ccgcgcgcga gcgagcggtt gtgcatccaa
     3781 tcggtccatg caaacgtcga caaccaatcc tccgaccagc ggaagcgtgg attgcatggc
     3841 ggtgacgagt ccgcctcgcg gattgcagcc cgcgccagaa tggccgacga ccaccacgcg
     3901 cgaaaggtcg attcggactt ggtcacgggt ggctcgaagg gtgtaagcca agaagtgatc
     3961 gaggtcgaaa tcgccccaaa gcgcttgcgg cagcttgcag gcggacgtcg acgaaggtgc
     4021 tgcgatcacg caaggaaaga aacgatggtc ggcaagggcc ctgtcccaac aaaagcgaa
//
LOCUS       DAOSOR010000534         4045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_22311_length_4045_cov_5.251378, whole genome shotgun sequence.
ACCESSION   DAOSOR010000534 DAOSOR010000000
VERSION     DAOSOR010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22311_length_4045_cov_5.251378"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /gene="aceE"
                     /locus_tag="PK710_21135"
     CDS             complement(<1..859)
                     /gene="aceE"
                     /locus_tag="PK710_21135"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HOT12296.1"
                     /translation="MFADPTTRLPDVDPVQTSEWMDSIAEVAKTQGHVRARYLASKLL
                     EHARSLGVQLPVLVQTPYINTINPSEEPPYPGDEKIEKRIRRLIRWNAAVMVVRANRR
                     FHGIGGHLATYASAASLYETGFNHFFRGREFPEGHDQILFQGHAAPGIYARAYLEGRL
                     TEGHLDRFRREQWLGHGLSSYPHPRLMPKFWEYPTVSMGLGPLFAIYQARFNRYLEAR
                     GMAKTSGNRVFAFLGDGEMDEPESLGALSLASRDKLDNLIFVINCNLQRLDGPVRGNG
                     KIIQELETVF"
     gene            complement(946..2166)
                     /locus_tag="PK710_21140"
     CDS             complement(946..2166)
                     /locus_tag="PK710_21140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12297.1"
                     /translation="MVAEAAVYLTSLMHRDDLFDITLRWLNNVPRQEDGRRLSEIFLF
                     ESAVSAPIVQDIMLGLFAKLFGERLSVERVQYKDLLRSRLIQGIPRYTPRMQELVTAY
                     QKSPEEFFPGMPVDAMLLSTPTRSLVALSRIKRPTRVAEKASFRLSDALADRVRQEAE
                     VFASARAFARGVALQHLISSETQMKEDFVAAEHVVAGAFKDGSVVFSREQLTINDAIG
                     FKVVGDEGELSFAEHLFAEEPGITIVEREEHRGHYNATNLLLDIDLPPIGKIIDENAH
                     RDWHQAAHRGLDPASAPRRFAEYVESGARTIRTEVILTTYPELIESELGRSLHELRIL
                     RLRDRQSYSGQIAQNARYLIEYLLALAYSPSVEVGELPIKLWGRYLPDTVLTAVGYLY
                     GIRQNGLLRWFLPD"
     gene            complement(2223..3620)
                     /gene="sthA"
                     /locus_tag="PK710_21145"
     CDS             complement(2223..3620)
                     /gene="sthA"
                     /locus_tag="PK710_21145"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HOT12298.1"
                     /translation="MPAGAEQRYDVVVIGSGPGGEGAAMKCAKGGLNVCMVERRPLVG
                     GACTHDATIPSKTLRQVIQSLVERKRIPFRGTISPMPSFPDLTRAAASVIERQVELRQ
                     GFYDRNRVPVLHGTARFVDPHTLHVEGADGVQVVQANAFVIATGSRPYHPTDVPFDHP
                     RVYDAESILGLSRTPESVTVYGAGVIGCEYASICRNLGVKVNLINTRSKLLTFLDDEI
                     IDALSYHLSDQGVVIRHGEQAQRVELLDDHVIMHLESGKRIKSDILLWANGRTGNTSE
                     LGLEKIGLVPDERGNLKVDDAYRTSVEHVYAVGDVVGFPSLASAAYDQGRMAGTHIVE
                     GRLDARLARNIPTGIYTTPEVSSLGRTEKELTLAKIPFEVGRAHFRHLARAQITGQTV
                     GMLKLLFHQQTLELLGIHAFGDQASEIIHIGQAIMAQEGSANTVAYFVNTTFNYPTMA
                     EAYRVAALNGMNRLS"
BASE COUNT          868 a         1081 c         1222 g          874 t
ORIGIN      
        1 ggaagacagt ttcgagttcc tggatgatct tgccattgcc gcgcacgggt ccatcgaggc
       61 gctgaaggtt gcagttgatg acgaagatca agttgtcgag tttgtctcgg ctcgcgaggg
      121 acagggcacc cagcgattcc ggctcgtcca tttccccgtc cccgaggaat gcaaatacgc
      181 gatttcccga tgtttttgcc attccccgcg cttcgaggta gcgattgaag cgcgcttggt
      241 agatggcgaa aagagggccc agacccatcg atacggttgg atattcccag aacttcggca
      301 tcaatcgtgg gtgcggatag gaagacaggc cgtgtccgag ccattgttca cgacgaaatc
      361 ggtcgaggtg gccttcggtg agacgacctt cgagataggc gcgagcgtag atgccgggag
      421 cggcatgtcc ttgaaacagg atctggtcgt gaccttcggg gaattcccgg cctcggaaga
      481 agtgattgaa ccccgtttcg tacaggctgg cggcggaggc ataggtggcg agatgcccgc
      541 cgatcccatg aaaacgcctg ttggcgcgca cgaccatgac ggccgcgttc catcggatca
      601 aacgtcgaat tcgtttctcg atcttttcat cgcctggata gggaggttct tcggatgggt
      661 tgatggtatt gatgtagggg gtttgcacga gcacgggtaa ttggactccg agcgagcgag
      721 cgtgttcgag caacttgctc gccaggtacc gtgcgcggac gtgtccttgg gtcttcgcca
      781 cctcggcgat cgaatccatc cattccgaag tctggacggg gtcgacgtca ggcaggcgtg
      841 tggtggggtc ggcgaacatg attcctccgt gggaaataat cgtaggctcg aggtaagaca
      901 gggcgatgta tggagcaggc ggggcccttc cgccagggtt gcggttcagt ctggaaggaa
      961 ccaccttagc aaaccgtttt gtcgaatacc gtaaaggtat ccgacagcgg taaggacggt
     1021 atcaggcagg tatcgtcccc agagtttgat gggcaattca ccgacttcga cggagggtga
     1081 gtaggccagg gcgagcaagt attcgatgag atagcgggca ttttgcgcga tctgaccgga
     1141 gtaggattgg cgatcgcgca agcgaaggat gcgcaattcg tgcaacgagc gaccgagttc
     1201 ggattcgatg agttcgggat aggtagtgag aatgacttcg gtccgtatgg tgcgggcgcc
     1261 cgattcgaca tattcggcga agcgtcgggg agcagaagcc ggatcgaggc ctcggtgcgc
     1321 cgcctgatgc caatcgcgat gcgcgttttc gtcgatgatt tttccaatcg gaggaaggtc
     1381 gatatccaga agaagattgg tggcgttgta atggccccgg tgttcctctc gctcgacgat
     1441 ggtgatgcct ggttcttccg cgaacaggtg ttccgcgaag gatagttctc cttcatcacc
     1501 gaccactttg aaaccgatgg catcattgat ggtgagttgt tcgcgggaaa acacgacgga
     1561 gccatctttg aaagctccgg ccacgacatg ttcggcggcg acgaagtcct ctttcatttg
     1621 cgtttcgctg ctgatgaggt gttgcaaggc aacaccgcgc gcaaacgcac gggcggatgc
     1681 gaagacttcc gcttcctggc gcacccgatc cgcgagagca tcggacaatc gaaacgaggc
     1741 tttctctgcc acgcgggtgg ggcgtttgat acggctcagg gcgaccaagc tacgcgtggg
     1801 tgtggaaaga agcatcgcat ccacgggcat tccggggaaa aactcctcgg gactcttctg
     1861 gtatgccgtc acgagttctt gcattcgcgg tgtgtaccgc gggatgcctt gaatgagtcg
     1921 cgaacgtaac aaatccttgt actgcacgcg ctcgacagaa agacgctctc caaacagctt
     1981 ggcaaacaat ccaagcatga tgtcttgcac gatcggcgca gacaccgcgc tttcgaacaa
     2041 aaaaatctcc gacagccgac gaccatcttc ctgtcgcggg acgttgttca accaccgcaa
     2101 cgtgatatcg aaaaggtcat ctcgatgcat caagctcgtc aagtacacgg cggcctcggc
     2161 aaccacgaat tcattggcct acagatggca tatccttagg aaatgacaaa tgaaatttca
     2221 ggtcaggaca accggttcat cccgttgagc gctgccacac gatacgcttc ggccatcgtt
     2281 ggatagttga aggtcgtgtt gacgaagtag gcgacggtat tagcgcttcc ttcctgcgcc
     2341 atgatggcct ggccgatatg aatgatctcg ctggcttgat cgccgaaggc gtgaattccc
     2401 aaaagctcca aggtttgctg atgaaatagc aattttagca ttcccacggt ctgccccgtg
     2461 atttgcgcgc gcgccaggtg acgaaaatgc gcacgcccca cctcgaacgg tatcttcgcc
     2521 aaggtaagct ccttttccgt tcttcccaag ctgctcacct ccggcgtcgt ataaattccc
     2581 gtcgggatat tgcgcgcaag acgcgcatcc agccgtccct ccacgatatg cgtacccgcc
     2641 attcgcccct gatcgtacgc ggctgacgcc aggctcggaa agcccaccac atcgcccacg
     2701 gcgtacacat gctcgacact cgtccggtac gcatcatcga ccttgaggtt cccccgttcg
     2761 tcaggcacca acccaatctt ttccaaacca agctcggagg tgttgcccgt gcgtccgttg
     2821 gcccaaagca aaatatccga cttgattcgc tttccgctct cgagatgcat gatcacgtgg
     2881 tcgtcgagca actcgactcg ttgcgcttgc tcgccgtggc gaatgacgac tccttggtca
     2941 ctcaagtgat aactgagagc atcgatgatc tcatcgtcga gaaatgtaag cagtttgctt
     3001 ctcgtgttga tgaggttgac cttgacgccg aggttgcgac agatcgatgc atactcgcag
     3061 ccgatcacgc ccgctccgta cacggttacc gactcgggag ttctcgatag ccctaaaata
     3121 ctctccgcat cgtagacgcg gggatgatcg aaggggacat cggtgggatg atagggacgg
     3181 gaaccggtgg cgatgacgaa ggcgttggct tgcacgacct gcacgccatc cgcaccttcg
     3241 acatgaaggg tgtgagggtc gacgaagcga gcggttccgt gcaacaccgg cacacgattg
     3301 cgatcgtaga agccttgtcg caattcgact tgacgttcga tgaccgaagc ggcggcgcgc
     3361 gtcaggtcgg gaaaggaagg catgggcgag atcgtgccgc gaaaaggaat gcgcttgcgt
     3421 tcgacgaggc tttgaatgac ctggcgaagg gttttggaag gaatggtggc gtcatgggtg
     3481 caggctccgc cgaccaaggg ccgacgttcg accatgcaga cgttcaagcc tcctttggcg
     3541 catttcatgg cggcgccttc tccccccggg cccgaaccga tcaccacgac gtcatagcgc
     3601 tgttccgcac ctgctggcat gctatccgac ctttgttatt ctgggttgcc tcgaagaata
     3661 cggcagttgg ttgtacttca tcgggccgcg aagtgtagtg gcaagccccg gtaattcacg
     3721 gctgggatgc ggcttgccct atatattcat aagtagtgga tatataagcg gaatattatg
     3781 ttgggaaggg cctagaggaa gcagggtgtt gccaagacgg caagggtaca aagaggctga
     3841 agatgttgtt gtgaggctgg cccatgtgta tcgcggcgtg gtgaccgggg cggatgtgga
     3901 attgggggcg acacgcagaa gagttcttcc ctcgccgttc gtgaggccga caaagccaac
     3961 catgtcggcg ttgaccccaa tcagcccatc aacgtatacc cgcgtggcta cggcatcccg
     4021 gccatgaatt catgaggcca gaaca
//
LOCUS       DAOSOR010000535         4040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_22348_length_4040_cov_4.464492, whole genome shotgun sequence.
ACCESSION   DAOSOR010000535 DAOSOR010000000
VERSION     DAOSOR010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22348_length_4040_cov_4.464492"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="PK710_21150"
     CDS             <1..1298
                     /locus_tag="PK710_21150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOT12299.1"
                     /translation="AIERYYNVGPSYDDVMADFDEGEIDFGEDNEEINVLELEKASED
                     APVVRLVNMILLSAIKKGASDIHVEPYEKTLRIRYRVDGVLMQEMTPPLKLRAALVSR
                     LKIMSSLDIAERRLPQDGRIKLKLGKGKEMDFRVSVLPTMWGEKVVLRLLDKSNLQLD
                     MTKLGFDPKPLGDFQWAINQPWGMVLVTGPTGSGKTTTLYSALSDLNKDGVNISTAED
                     PVEYNLPGINQVQMHEEIGLNFAMSLRSFLRQDPDIIMVGEIRDFETAEIAVKAALTG
                     HMVLSTLHTNDAPSTISRMLNMGVEPFLITASVNLVLAQRLARRVCKDCATASVLDRK
                     VFEELGFSDELMENATIMKGAGCRTCNNTGYKGRVALYEVMRMTENLRETVLQGASTA
                     ELKVAAIKNGMISLRAAGNLKIAQGVTTPEEVLRVTMAD"
     gene            1332..2465
                     /locus_tag="PK710_21155"
     CDS             1332..2465
                     /locus_tag="PK710_21155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOT12300.1"
                     /translation="MSTGSPEGLKVNLHQLLRAMIEKGASDMHITTGSPPLLRIDGAI
                     VPLKLPALSPVETKQLCYSVLTEEQKVSFEKRNETDLSFGVRGLSRFRANIFMQRGAV
                     AGAFRSIPFKILTFEELGLPPVIAELSAKPRGLVLITGPTGSGKSTTLASIIDKINSE
                     TRQHIITVEDPIEYLHPHKLSVVNQREIGTDTVNFKDALKYILRQDPDVVLIGEMRDL
                     ETIESALTIAETGHLVFATLHTNSCVQAINRVIDVFPSHQQSRVRAQLSFVLQGVVTQ
                     LLLPRASGPGRVLALEVMVPNPAIRNLIREDKIHQIYSQLQVGQEKHGMQTLNQSLYS
                     LYVRRLISLEEAMGRSSDQDELRAMIEGRVAPMGPQGTAMPRG"
     gene            2669..>4040
                     /gene="purF"
                     /locus_tag="PK710_21160"
     CDS             2669..>4040
                     /gene="purF"
                     /locus_tag="PK710_21160"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOT12301.1"
                     /translation="MCGIFGIFGHPEAANITYLGLHALQHRGQESAGIVTTDGRQMFV
                     HRHLGHVADAFSETDLQRLPGHAAIGHVRYSTSGDSHIRNAQPFAVDYARGSIAVAHN
                     GNLTNARNLREHLEARGSIFVSNSDTEVIVHLIATSTQRAVQDRIEEAMAHARGAYSL
                     VFLTEENLIAVRDPYGFRPLCLGELDVASSREKAVLIASEGTSFDLVGARFVREIRPG
                     EMVSIDRKAIRSTMPDLLAGLSVDHRLCIFEYVYFARPDSVINGNSVYTVRKAFGAKL
                     AEEHESQADLVVPVPDSGVPAALGYAERAGLPFEMGLIRSHYVGRSFIEPQQTIRHFG
                     VRLKFNAVASHLRGKRVIVVDDSIVRGTTIKKILLMLRHAGAKEVHVRIASPATLWPC
                     YYGIDTPTRSELIASTRSNDDIRQFIGADSLGFLSMEGMLSVVDHSKDATRVCSSFCD
                     ACFSGHY"
BASE COUNT          894 a         1044 c         1154 g          948 t
ORIGIN      
        1 cggccatcga gcgttattac aacgtcggac cctcgtacga cgatgtgatg gcggactttg
       61 atgaagggga gatcgacttt ggagaggaca acgaagagat aaacgtattg gagcttgaaa
      121 aggccagtga agatgctccg gtcgtgcgtc tggtgaatat gattttgctg tccgccatca
      181 agaagggggc gagcgatatt catgtcgagc cttacgagaa aacgcttcgc attcggtatc
      241 gcgtggacgg agtcttgatg caggagatga cgccgccgtt gaagttacgg gcggcgttgg
      301 tcagccggtt gaagatcatg agttcgctgg atattgccga gagaaggctt ccgcaggacg
      361 ggcggatcaa gctgaagctt ggcaagggca aggagatgga cttccgcgtg tcggtgcttc
      421 ccacgatgtg gggagagaaa gtggttttgc gtcttttgga caagtcgaac ttgcaactcg
      481 acatgaccaa gcttgggttc gatccgaagc cgctagggga ttttcaatgg gccatcaatc
      541 aaccctgggg catggtgttg gtgacggggc cgacggggtc tggcaagacg acaacattgt
      601 attccgcgct ttccgatctc aacaaggatg gggtgaatat cagcacggcg gaagacccgg
      661 tggaatacaa ccttccgggg atcaaccagg tgcagatgca tgaggaaatc ggcttgaatt
      721 tcgcaatgtc gctgcgatcg tttcttcggc aagatccgga catcatcatg gttggcgaga
      781 tccgcgactt tgaaaccgct gaaattgctg tcaaggcagc gttgacgggt cacatggttc
      841 tgtcgacgct tcacaccaat gatgctccgt cgaccatttc tcgtatgctc aacatgggtg
      901 tggagccttt tctcatcacg gcgagtgtga acttggtgtt ggcacaacgt cttgctcgtc
      961 gtgtttgcaa ggattgcgcg accgcgtccg tgttggatcg aaaggtattc gaagagcttg
     1021 gtttttccga tgagctgatg gagaatgcca cgatcatgaa aggggctggc tgtcgtacct
     1081 gtaacaacac cgggtacaag gggcgtgtgg ccttgtacga agtcatgaga atgaccgaaa
     1141 acctacggga aacggtgctt cagggggctt cgacggcgga actcaaggtg gcggctatca
     1201 aaaacgggat gatatccttg cgtgctgccg gcaacctcaa aatcgcgcaa ggcgtaacga
     1261 cgccggaaga agtccttcgc gtcacgatgg ccgattgatc gtaggaacaa gcagcgaaag
     1321 ggacgaatac catgtcgacg gggtcacctg agggtttgaa ggtcaatctg catcaactgc
     1381 ttcgcgccat gattgagaaa ggcgcgagtg acatgcacat taccaccggg tctccgccgc
     1441 tattacggat tgatggcgcg atcgttccgc tcaaacttcc cgcgttatct cccgtggaaa
     1501 cgaagcagct ttgttattcg gttcttacgg aagaacagaa agtatcgttc gagaaacgca
     1561 acgagacgga tttgtctttc ggtgttcgcg gcctttcacg ctttcgggcc aacatcttca
     1621 tgcagcgggg agctgtcgcc ggcgcttttc gatcgatccc attcaaaatt ctgaccttcg
     1681 aggaacttgg tcttcctccg gtcattgccg agttgtccgc gaaaccgcgg ggcctcgtcc
     1741 tcatcaccgg gcccaccggc tctgggaagt caacgacgct tgcttccatc atcgacaaga
     1801 tcaacagcga gacccgtcag cacatcatca cggtcgaaga cccgatcgaa tatcttcatc
     1861 cccataagct gtccgtagtc aatcagcgtg agattggaac cgatacggtc aacttcaaag
     1921 atgctctgaa atatattctg cgacaggatc ccgatgtcgt tctcattggc gaaatgcgcg
     1981 acctcgaaac catcgaatcg gcgttgacga ttgcggaaac cgggcacctc gtgtttgcca
     2041 cgctgcatac caactcttgc gtgcaggcca tcaaccgcgt catcgacgtg tttccgtccc
     2101 atcaacaatc acgcgttcgc gcgcagttgt cgttcgttct tcagggggta gtcacacaat
     2161 tgctgttgcc cagggcgagt ggccctggtc gtgtccttgc tctcgaggtc atggtgccga
     2221 accccgcgat ccgaaacctc attcgtgagg acaagattca tcaaatctac tcgcaattgc
     2281 aggtggggca ggaaaaacac ggcatgcaaa cgctcaacca atccctgtat tcgctgtatg
     2341 tgcgtcgcct catttcgttg gaggaggcga tgggtcgatc ttcggatcag gacgaattgc
     2401 gtgcgatgat tgagggacgc gtggctccca tggggccaca aggaaccgcg atgccgcgtg
     2461 gctgagaaag caatgacgcc gtgtcgtcgt gtggctatcc catgatccgg tgataggcac
     2521 gtcatcggca tctcgatgag atgcggaacc agagaagatc ccaaccagaa aatgccgcgc
     2581 ggttgaactt ctattctctc gttgtgccta tccatctgtt gttggctagc gcgtaccttt
     2641 tctcgaccac ctgtactatg aggcgcgcat gtgcggcatc ttcggcatct tcggtcatcc
     2701 agaagcagcc aacattacct atctaggtct tcatgctctt caacatcgag gtcaggagtc
     2761 cgccgggatc gttaccaccg atggacgaca gatgttcgtt catcggcacc tgggccatgt
     2821 ggccgacgct ttttccgaga ccgatcttca acgcttgccc gggcatgcgg ccattggaca
     2881 cgttcgatac tcgacgtccg gggatagcca cattcgaaac gcgcaaccgt ttgcagtcga
     2941 ctatgcgcgc gggtcgattg cggtagccca taacggcaat ctcaccaacg cacgcaatct
     3001 tcgtgagcat ctggaagcgc gtggttcgat cttcgtttcc aattcggata cggaggtgat
     3061 cgttcacttg atcgcgactt ctacacaaag agcggttcaa gatcgcatcg aagaagccat
     3121 ggctcatgcc cgtggcgcgt acagcctcgt gtttctcacc gaagaaaatc tcatcgcggt
     3181 gcgagatcct tacggctttc gacctttgtg tcttggtgaa ctcgatgtcg cctcatcgcg
     3241 ggaaaaggca gttctcattg cgagtgaagg cacgagcttc gaccttgtgg gtgctcgatt
     3301 cgtgcgtgag attcgacctg gggaaatggt ttcgatcgac cgaaaagcga ttcgatccac
     3361 gatgccggat cttctcgccg gcctttcggt ggaccaccga ttatgtattt tcgagtacgt
     3421 gtactttgct cgtccggact cggtcatcaa tggcaatagc gtgtacacgg tccgaaaagc
     3481 attcggcgca aaactagccg aagaacatga gtcacaagca gatctcgtcg ttccggtccc
     3541 ggattccggg gttcctgccg ccctggggta cgcggagcgt gcaggcttgc ccttcgaaat
     3601 ggggttgata cgaagccatt acgtcggacg atccttcatc gagccgcagc aaaccattcg
     3661 gcactttggc gttcgtctga aattcaatgc cgtagcaagt catttgcgag gtaaacgagt
     3721 catcgtcgtg gacgattcga ttgtgagggg gaccaccatc aaaaaaattc tcttgatgct
     3781 tcgtcatgcc ggagccaaag aggttcacgt ccgaattgcg agcccggcta ccctatggcc
     3841 ttgttattat gggattgaca cgccaacgcg aagtgaactc attgcctcga ctcgctcgaa
     3901 cgatgacatt cgacaattca ttggcgcgga ctcgctcggg ttcttgtcga tggaaggaat
     3961 gctgtcggtg gtggatcatt cgaaggacgc gacgagagtc tgttcgtctt tttgcgatgc
     4021 gtgtttttca gggcattacc
//
LOCUS       DAOSOR010000536         4029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_22441_length_4029_cov_3.871163, whole genome shotgun sequence.
ACCESSION   DAOSOR010000536 DAOSOR010000000
VERSION     DAOSOR010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22441_length_4029_cov_3.871163"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            321..590
                     /gene="rpsO"
                     /locus_tag="PK710_21165"
     CDS             321..590
                     /gene="rpsO"
                     /locus_tag="PK710_21165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOT12302.1"
                     /translation="MALTAESKNNIVATYRRHETDTGSPEVQIALITKRITYLAEHFK
                     NHSKDHHSRRGLLKLVGKRRRLLDYLKRKDMDRYRKVVADLGLRK"
     gene            746..3304
                     /gene="pnp"
                     /locus_tag="PK710_21170"
     CDS             746..3304
                     /gene="pnp"
                     /locus_tag="PK710_21170"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOT12303.1"
                     /translation="MFKNRGRRRSGRPARAVSFPAQKEATIAMFVRESIRVGNAELTL
                     ETGRLAKQASGAVLVTMGETVLLVTAVSSGEERPGIDFFPLTCDYIEKTYAAGKIPGG
                     FFKREARPRDYEVLTARLMDRPVRPLFPEGFKVETQIIATLWSFDKEHPAEPLALIGA
                     SAALHISDIPWEGPIAGIRVGRVDGELIAFPTYQQLELSDIDITVACSKEAIVMVEGA
                     TKEVSEQVLVDALMFAHESAQPIIHLIERLRQSVGKPKYPFQSVRLPEELKKRLFDHC
                     VGGVRACLQIADKQSRYAQYKVVRKEAFAKLIEEFGEETINTWEKHLKESFEDDVKGR
                     EMRDMILDKGLRIGGRDTRTIRDIRCEVGVLPRTHGSALFQRGETQALVTATLGTSSD
                     EQKVDTLMGDTYKSFLLHYNFPPFCTGEVKRIGSVGRREVGHGNLAERALQGMIPDHE
                     AFPYTTRIVSEILESNGSSSMATVCGGTLSLMDAGVPIKAPVAGIAMGLIKDGDRIAI
                     LSDILGDEDHMGDMDFKVCGTARGITAIQMDIKVKGLSRNVLAAALDQAREGRLFILG
                     KMLESLPAPRENMSKWAPRITTIKVKPDQIRIIIGSGGKTIKGIVDQTGVQIDVEDDG
                     TVNVASSDESAVRRALEIIQGLIAEPEVGVVYKGTVKRITDFGAFVEILPNTDGLLHI
                     SEIAHERIERVEDVLKEGDSIEVKVISVERDGKVRLSRRVLLPGGDSSERPRPDAPPR
                     TRNERTTTRERGGPPRERSRTPPPREPRPAREGRSESVERPAREPREKPVKMPRSEAS
                     EMTRPPREPRDRIEKESTPETHDTPVRARAPRERRSEPSGDSAAPRRRREPRDS"
     gene            3520..>4029
                     /locus_tag="PK710_21175"
     CDS             3520..>4029
                     /locus_tag="PK710_21175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12304.1"
                     /translation="MWLAQSVIPPVPPFARLPAHVVDAASDQFIGLDGQYSAPLLARA
                     FRKCRVSQPSLAAWLEQRLAGRIDDAARGFGASMAMSIWMAFTLFAGTNLGTVDPQDF
                     RNVEELLRTDEERRCNDPDEIIESDDIIAAHQPEIARLIRNKLDETLSNYADDVDVDD
                     VDMIYRMLLV"
BASE COUNT          950 a         1262 c          997 g          820 t
ORIGIN      
        1 gaagtccgcc tcgacgcaac aacagttcaa tcaacgcttt cgtctcgcct tcccaagcgt
       61 tacgaaccaa ttcttccacg gaagcaccag gacaaggacg cgtcatgagg atgggtagca
      121 tgcggacaat tcaagcgctg gagcaaggtt tcatcgccaa cgagttgccg ccaacgctca
      181 atctgtcctt tggactctcc caatttcgat tgaccattcc atgaccatgc gtatactccc
      241 gtttctcgaa attggcctgg tccggggctg tgcatggttg cacctggctc tcaccaaggc
      301 gtcacagaag gccgatgtcc atggctctca ccgctgaatc aaaaaacaat attgtggcaa
      361 cctatcgtcg ccacgaaacc gataccggct ccccagaagt acagatcgcc ttgattacca
      421 agcgtatcac gtaccttgcg gaacacttca agaaccactc caaagaccac cattcccgtc
      481 gaggcctgct caaattggtt ggcaaacgac gtcgtcttct tgactacttg aaaaggaaag
      541 acatggatcg gtaccgcaaa gtggtggccg atctcggctt gagaaaatag gttgccaacg
      601 gctcacggta ccagtgccta tcgtgggctt gtgatacccg cataatcgcc catgctcgac
      661 atgcatgggc caaccccaac ccccggacga tccctttgag ccacttcatc ttcgcttcgt
      721 ggacacgaac taccacgatc gcttcgtgtt caagaaccga ggaagaaggc gttctggtcg
      781 tccggctcgc gctgtgtcct ttccagcgca gaaagaggcg accatcgcca tgttcgtgag
      841 agaatccatt cgtgtcggca acgccgaact cactctagaa acaggccgtc ttgcaaaaca
      901 agccagcggc gccgtcctcg ttaccatggg agaaaccgtt cttctggtga cggctgtctc
      961 ctcgggggag gaaagacctg gaatcgactt ttttcccctg acttgcgatt acatcgaaaa
     1021 aacctatgcc gcaggaaaaa tccccggcgg ctttttcaaa cgggaagcaa gacctcggga
     1081 ttatgaagtc cttaccgcac ggctcatgga ccgccccgtg cgacccctct ttcccgaagg
     1141 cttcaaagtc gaaacccaaa tcatcgctac cctctggtcc ttcgacaaag agcaccccgc
     1201 tgaacccctt gccctcatcg gcgcctccgc cgctttgcat atctccgata tcccgtggga
     1261 aggccccatc gctggaatcc gcgtcggacg cgtcgatggc gagttgatcg ctttccccac
     1321 ttaccaacaa ctcgaactca gcgacatcga catcaccgtc gcctgctcca aagaagccat
     1381 cgtcatggtg gaaggcgcta ccaaagaggt ctccgaacag gtccttgtcg atgccctcat
     1441 gttcgcgcac gaatccgccc aacccatcat ccatctcatc gaacgactcc gacaatccgt
     1501 cggaaaaccg aaatacccct tccaatccgt gcgtctgccc gaagaactca aaaaacgcct
     1561 tttcgaccat tgcgtcggcg gcgttcgcgc gtgccttcaa atcgctgaca aacaatcgcg
     1621 atacgcacaa tacaaggtgg tccgcaaaga ggctttcgcc aaactcatcg aagaattcgg
     1681 cgaagaaacc atcaacacat gggaaaaaca cctcaaagaa tcattcgaag atgatgtcaa
     1741 aggccgcgag atgcgcgaca tgattctcga caaaggcctg cgaatcggag gacgcgatac
     1801 ccgaaccatc cgcgatatcc gttgtgaagt cggcgttctc ccccgcacgc atggctccgc
     1861 cctctttcaa cggggcgaaa cgcaagccct cgttaccgct accttgggaa ccagctccga
     1921 tgagcaaaaa gtcgataccc tcatgggaga tacctacaaa tcgtttctcc ttcattacaa
     1981 cttccccccg ttctgcaccg gagaagtcaa gcgaatcggt tccgtcgggc gacgagaagt
     2041 agggcatgga aaccttgctg aacgcgcgct gcaagggatg attcctgacc acgaagcctt
     2101 cccctatacc acccgcatcg tcagtgaaat cctcgaatcc aacggctcct catccatggc
     2161 taccgtttgc ggtggcacct tgagcctcat ggatgctggc gttcccatca aagctcctgt
     2221 cgcagggatc gccatggggc tcatcaagga cggagacaga atcgctattt tgtccgatat
     2281 ccttggcgat gaagatcaca tgggcgacat ggacttcaaa gtctgtggca cggctcgagg
     2341 gattaccgcc atccagatgg atatcaaagt caaaggccta tcccgaaacg tactggccgc
     2401 cgctttggat caggctcggg aggggcgtct gttcatcctg ggaaaaatgc tcgaatcgct
     2461 gcccgcccca agagaaaata tgtccaagtg ggcacctcga attaccacca tcaaagtcaa
     2521 acctgaccaa atccgtatca tcatcggttc cggtggcaaa accatcaaag gaatcgttga
     2581 ccaaaccggt gtccaaatcg atgtcgaaga cgatggaacc gtcaatgtgg cttcttcgga
     2641 cgaaagcgct gtccgacgag ccctcgagat tattcaagga ctgattgcgg aaccggaagt
     2701 gggcgtcgtg tacaaaggga ccgtcaagcg catcaccgac ttcggtgcct tcgtcgaaat
     2761 cctccccaat accgatggcc tgttgcatat ctccgaaatc gcccacgaac ggattgaacg
     2821 cgtcgaagat gtcctgaaag agggcgacag catcgaggtc aaggtcatca gtgtcgaacg
     2881 agatgggaaa gtgagactca gtagacgtgt tcttctgcca ggaggcgact cttcggaacg
     2941 tccgcggccc gatgctccgc cccgcacgcg caatgagcgc acgacgacac gggaacgggg
     3001 tgggcctccc cgtgaacgct cccgaactcc tcccccccgc gagcctcgcc ccgcccggga
     3061 gggccgaagc gaatcggtcg aacgacccgc gcgcgaacct cgggaaaaac ccgtaaaaat
     3121 gccgcgttcc gaagcctcgg aaatgactcg tcccccgcgc gagcctcgcg accgcatcga
     3181 aaaagaatca actcccgaaa cccatgatac ccccgttcgc gcacgggcgc ctcgggagcg
     3241 acgaagtgag ccttccggtg attctgcggc cccccgtcgc cgtcgagaac cccgcgatag
     3301 ctgatccctc ccaagcggag acaacctgct acgtttctca ccgtgcgttg gtccgaacgg
     3361 tataaatatg ctttaaattc aatatgttgg cctgattggg cacaggcatt ctcaaaatcg
     3421 ctttcctttc cctccgcgct ttgcccctgc cgttcgctcc ccacccatac ccagtctacc
     3481 cattcccttt tacctcccca atgctatcct gcgcgcatct tgtggctggc ccagtcggtc
     3541 attcccccgg ttcccccctt cgcgcgactt cccgctcatg tggttgacgc ggcttcggat
     3601 cagtttatcg gcctagatgg tcaatacagc gctcctttgc tcgcccgtgc gttccgaaaa
     3661 tgccgggtca gccagccatc tcttgccgct tggctcgaac aacgattggc tggacgtatc
     3721 gacgatgcgg caagaggctt cggtgcgtcg atggccatga gcatctggat ggcattcacc
     3781 ctatttgccg gaaccaacct cggtaccgtc gacccgcagg atttccgcaa tgtggaagaa
     3841 ctactccgca ccgacgagga acgtcgttgc aatgaccccg acgagatcat cgagtccgac
     3901 gatatcatcg ctgcccatca accggaaatc gcgcgattga tacgcaacaa gctggacgaa
     3961 accttatcga attacgcgga tgacgtggat gtcgacgatg tcgacatgat ttatcgcatg
     4021 ctgctggtt
//
LOCUS       DAOSOR010000537         4027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_22454_length_4027_cov_4.771652, whole genome shotgun sequence.
ACCESSION   DAOSOR010000537 DAOSOR010000000
VERSION     DAOSOR010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22454_length_4027_cov_4.771652"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..113
                     /locus_tag="PK710_21180"
     CDS             <1..113
                     /locus_tag="PK710_21180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12305.1"
                     /translation="MSIELITPVALEEQMRFAIREGGRTVGAGIVTEIVE"
     gene            124..435
                     /gene="rpsJ"
                     /locus_tag="PK710_21185"
     CDS             124..435
                     /gene="rpsJ"
                     /locus_tag="PK710_21185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOT12306.1"
                     /translation="MADTTKIRIRLRAFDHQLLDKSTSDIVETAKRTGARVAGPIPLP
                     TEIRRYTVLRSPHADKKSREQFEIRTHKRLLDILDPTQQTLDALMKLDLSAGVDVEIK
                     S"
     gene            451..1101
                     /gene="rplD"
                     /locus_tag="PK710_21190"
     CDS             451..1101
                     /gene="rplD"
                     /locus_tag="PK710_21190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOT12307.1"
                     /translation="MPTVDVYNLAREKVGSLELSDEVFDTEVKEHLFYEVVKAQLASR
                     RSGTASAKERAAVRGSSKKIYRQKGTGRARHGSIRAPIFVGGGRAHPPQPRDWSYKPP
                     RKVRLSALKSALSMFCKEGRLIVVDSLAIEEVKTKKMVETLACLQVDKKTLVVDHKDN
                     DKLRLSVRNLQTTQFLPPEGVNVYDLLRHDHLVVSRDAVKALEARCLAKRSQGETS"
     gene            1098..1388
                     /gene="rplW"
                     /locus_tag="PK710_21195"
     CDS             1098..1388
                     /gene="rplW"
                     /locus_tag="PK710_21195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOT12308.1"
                     /translation="MTPEQTIDKPIILTEKATRIRGENKVIFKVARKANKIQIRDAIQ
                     KLFDVTVTDVNTIVMRGKERRMGRGHARLQNWKKAIITLKEGDSIPFFDASE"
     gene            1407..2237
                     /gene="rplB"
                     /locus_tag="PK710_21200"
     CDS             1407..2237
                     /gene="rplB"
                     /locus_tag="PK710_21200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOT12309.1"
                     /translation="MGIKTYKPTSPARRFYSSSDFGEITKTKPERSLTEGKISTGGRN
                     NYGRITARFRGGGHKRRFRTIDFRRNKIGVPARVAAVEYDPNRSARIALLHYVDGEKS
                     YILCPDGLNVGDTVVSSRHADIKPGNAMTLRHIPMGTAIHNIEMKRGKGGQIVRSAGT
                     FAQLMAKDGDYAQVRLPSGEVRKIHLDCKATIGHVSNLDHAQIIIGKAGRSRWLGRRP
                     HNRGVSMNPVDHPMGGGEGRSSGGRHPCSPWGQLSKGLKTRKNKRTDSMIIKHRSKKG
                     "
     gene            2241..2531
                     /gene="rpsS"
                     /locus_tag="PK710_21205"
     CDS             2241..2531
                     /gene="rpsS"
                     /locus_tag="PK710_21205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOT12310.1"
                     /translation="MARSIKKGFFIDGHLLEKINEAANQKAKKVIKTWSRRSTIIPEA
                     VGLTFAVHNGRKFVPVFITENMVGHKLGEFAPTRTYHGHSGDRKGKVAVKKK"
     gene            2709..3572
                     /locus_tag="PK710_21210"
     CDS             2709..3572
                     /locus_tag="PK710_21210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12311.1"
                     /translation="MTCTRSLLLAAAIALPCCDSESRAASQGVDDTRNPGEILLDLRD
                     DARLYAMVQPVPAQSDAPRTLMVRWVKQGQAQPASWAFADSPVLDARLVPHSEAIIVV
                     TQNRDLMLLTSIDASPVVLDSRVHPPLSLSENGQFLAYAQGVIPDLEITRFDLNKRSR
                     LESTQNMAPAWSPALSENGSRLLFVSGSTGYPELWEYRGNQAVRLTDRNLTPIPFPSG
                     PTAPIWNGNTIVFEDTTGVHLLSIEPLRLLGSLQGTLPVGVPRSQRFIVQDTTENRWR
                     WMSFDGTEVTP"
     gene            3569..>4027
                     /locus_tag="PK710_21215"
     CDS             3569..>4027
                     /locus_tag="PK710_21215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT12312.1"
                     /translation="MRSLYSGLLVLTPAMLSFLFAVDASATKLRRPFVADLSFNYGFD
                     NNGSAAGCTDHECGSRCYNGHGGNDYALPLGTDVLAPADGVVVATHDGCPNYGGLGNT
                     CGTGCGNYVKIKLADGNFVLFCHFQLGSLKVANGDSVKCGQVVGKSASSGN"
BASE COUNT          918 a         1195 c         1100 g          814 t
ORIGIN      
        1 cgatgagcat tgaactcatc acgccggtgg cgctcgaaga gcagatgcga tttgccattc
       61 gagaaggtgg tcgcacggtg ggtgcgggca tcgtgaccga gatcgtggag taggaatagt
      121 tccatggctg atacaacgaa gattcggatc cgccttcgag cattcgatca ccagcttctc
      181 gacaagtcga cgtcagacat tgtggagacg gccaaaagaa cgggtgcgcg tgtggcagga
      241 cccattcctt tgcccaccga aattcggcgc tataccgtat tgcgcagccc ccatgcggat
      301 aaaaaatccc gggagcaatt cgagatccgc acgcataagc gtcttctcga cattctcgat
      361 cccactcaac aaaccctcga tgcgttgatg aagctggacc tttcggctgg agtcgacgtc
      421 gagatcaagt cgtagccgcg ggaggacgcc atgccgacag tggatgtcta caatctcgca
      481 cgtgagaagg ttggttcgct cgaattgagc gacgaagtct tcgacacgga agtcaaagag
      541 catttgtttt acgaagtggt caaagctcag cttgcatcgc gccgatcggg cacggcttcg
      601 gccaaggagc gagcggccgt taggggctct tcgaagaaga tctatcgcca aaagggcacg
      661 ggccgcgcac gtcacggttc cattcgtgct cccattttcg tgggcggtgg cagggcgcat
      721 ccgccccagc cgagggattg gagctacaaa ccgcctcgca aggtgcgtct gagcgccctg
      781 aagagcgctc tatccatgtt ctgcaaagaa ggtcgcctca ttgtggtgga ctctttggcg
      841 atcgaggaag tcaaaaccaa aaaaatggtc gagaccctcg cttgcctcca agtcgacaag
      901 aaaaccttgg tggtcgacca caaagacaat gacaagctcc gcctctcggt caggaacttg
      961 caaacgactc agttcctacc tcccgagggc gtcaacgtct acgacctgct ccgccacgac
     1021 cacctcgtgg tgagccgcga tgcggtcaag gcactcgaag cccgctgcct cgcgaaacga
     1081 tcgcaaggag agacgtcatg actcccgagc agaccatcga caaacccatt atcttgaccg
     1141 aaaaagccac gcgaatccgt ggggaaaaca aggtcatttt caaggtcgcc cgcaaggcga
     1201 acaaaattca aatccgagac gccatccaga aactgttcga tgtcaccgtg accgatgtca
     1261 acacgatcgt catgcgcgga aaagaacggc ggatgggccg aggacacgcg agattgcaaa
     1321 attggaagaa ggcgatcatc accttgaaag agggagattc gatcccgttc ttcgacgcct
     1381 cggagtaggc aggaaggtag cttgccatgg gcatcaaaac ctataagccc acgtcacccg
     1441 cgaggcgctt ctattcgtcg agtgacttcg gggagatcac aaaaaccaaa cccgaacggt
     1501 cgttgaccga ggggaaaatc tccaccggtg ggcgcaacaa ctacggccgt atcaccgctc
     1561 gcttccgtgg gggaggacac aagcgacgct ttcgaaccat cgactttcgt cgcaacaaaa
     1621 ttggcgtgcc cgcacgtgta gcggcagtgg aatacgatcc gaaccgcagc gcaaggatcg
     1681 ctcttttgca ttatgtcgat ggggaaaaga gctatatcct ctgccccgac ggcctcaacg
     1741 tcggagatac ggtggtgtcc agccgtcacg cggacatcaa accgggcaac gccatgacgc
     1801 ttcgccatat cccgatgggg acagcgattc acaacatcga aatgaagcga ggcaaaggcg
     1861 gacaaatcgt tcgatccgcc ggcaccttcg ctcagctgat ggcaaaagat ggcgattacg
     1921 cccaggtgcg cctgccttcc ggagaggttc gcaagatcca tctcgattgc aaagccacca
     1981 tcgggcatgt gtccaacctc gaccacgctc aaatcatcat cggcaaagct ggccgctcac
     2041 gctggcttgg ccgacgtcct cacaaccgcg gcgtgtccat gaaccccgtc gaccatccca
     2101 tgggcggtgg cgaaggccgt agcagcggtg gacgacatcc gtgctcaccc tggggccagc
     2161 tcagcaaagg cctcaagacc cgaaagaaca agcgcaccga cagcatgatc atcaagcatc
     2221 ggtccaagaa gggataactc atggcccgct ccatcaagaa aggattcttc atcgacgggc
     2281 atctgctcga gaagatcaac gaggccgcga accaaaaggc gaaaaaggtc atcaagacct
     2341 ggtcgcgacg ctcgaccatc attcccgaag ccgttgggct gacctttgcc gtccataacg
     2401 gacgcaaatt cgttcccgtg ttcatcacgg aaaatatggt cggccacaag ctcggtgagt
     2461 tcgctcccac gcggacgtac cacggccatt cgggagatcg taagggcaag gttgccgtga
     2521 agaagaagta gtcgttcgct gcagttttca gttccatttc ttacggcgaa tctcgccaac
     2581 ccatcatgac cccgtcatgc ttcggggttc cgtgtctttc gaatacgaga caaggtcacg
     2641 tttcaccccc tctgaaggag tcaagcgcca ccgtaaggct cgcgttcttt ccgtgctatc
     2701 cttgccgcat gacttgcacg cgtagtctcc tgcttgccgc cgccattgcc ctgccttgct
     2761 gcgattctga atcgcgcgcc gcctcacagg gggtcgacga tacgcgcaat cccggcgaaa
     2821 tcttgttgga tctacgggat gacgcccggc tctatgccat ggttcagccg gttccggctc
     2881 agtccgacgc accgcgaacc ttgatggtgc gatgggtgaa acaaggccaa gcccagccag
     2941 cctcatgggc cttcgctgac tcgcccgtcc tcgatgcacg gttggtgcct cattcggaag
     3001 ctatcatcgt cgtcacccag aaccgcgacc tgatgctgct tacctcgatc gatgcctcgc
     3061 ccgtggttct cgattcacgc gtgcatccac ccttgtcact atccgaaaat ggacagtttc
     3121 tggcctacgc ccagggcgtc attcctgatc tcgaaatcac tcgtttcgat ctgaacaagc
     3181 gctctcgtct cgaaagcacg cagaacatgg cgcccgcctg gagccccgca ttatccgaaa
     3241 atggatcgcg actgctattc gtctctggtt ccaccggcta tcccgaactg tgggaatacc
     3301 gaggcaacca agccgttcgt ctcaccgacc gtaacctcac gccaatccca ttcccctctg
     3361 gtcccaccgc tcccatctgg aatggcaata ccatcgtctt tgaagacact acgggcgttc
     3421 acctactgtc gatcgaacct cttcgtttgc ttggttctct gcaaggaacc ttgccggtcg
     3481 gtgtcccccg atcgcaaagg ttcatcgtgc aagacaccac ggaaaatcgg tggcgttgga
     3541 tgtcgttcga cggcacggag gtgacgccgt gagatctctt tattctggcc ttcttgtgct
     3601 cactcccgcg atgttgtcgt ttctattcgc cgtggacgcg tcggcgacca agcttcgaag
     3661 accctttgtg gcggatttgt cgttcaacta tggatttgac aacaacggtt ccgcggcggg
     3721 ctgcaccgac catgaatgcg gttcgcgttg ctacaacgga catgggggaa acgattatgc
     3781 attgcccctt ggaaccgatg tgctcgcgcc ggccgatggg gtcgtggtgg caacccatga
     3841 tggttgtcca aactatggcg gcctgggcaa tacgtgcgga acgggttgcg gcaactacgt
     3901 caaaatcaaa ctcgcggatg gcaacttcgt gctgttttgc cacttccaac tcggctcttt
     3961 gaaagtcgcc aatggcgata gcgtcaagtg cggccaagtc gtgggtaaat ccgcgagttc
     4021 tggcaat
//
LOCUS       DAOSOR010000538         4009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_22597_length_4009_cov_4.790339, whole genome shotgun sequence.
ACCESSION   DAOSOR010000538 DAOSOR010000000
VERSION     DAOSOR010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22597_length_4009_cov_4.790339"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..1608)
                     /locus_tag="PK710_21220"
     CDS             complement(118..1608)
                     /locus_tag="PK710_21220"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT12313.1"
                     /translation="MATSMGGNDSFRRSVPRTTNMPDGHHQQRYRVIDKIAAGGMAEV
                     FRAESAGIEGFKKKVAIKRVLPHLSQKRKFMSMFLDEARLSAHLSHSNVVQVFDIGIG
                     DDTYFIVMEYVDGADLKAIIEMLRKRGQPFPLEAAVYVATKICEGLAYAHDLKDGRGT
                     PLGIVHRDMSPPNVLITRYGEIKITDFGLAKANNQLEKSEPGIIKGKFSYLAPEAAMG
                     HDVDARADIFAVGIILWEMLAGRKLFLGESDYATVKLVQQANIPSLPEINHKVPPGLE
                     RIILRSLARDLNQRYGHALALAEDLNGFLYHHGQPVGAWDIARLVQQALDERQRQKPA
                     SANIIDQLIKQALFEFTSLGTSQEQSGASPSGRTTPTGTPSPHDGSMITDWESEIQIH
                     NPYGIGRTGANDKLLSSLQDLQVGNLSALEDMPTGPNPLENSLQPASSPPSPPSIESS
                     RPSMDSPTPTEPAAKKKGLGLIIAIVVLLLGAAAAAGAYFAQTVPQ"
     gene            complement(1785..2441)
                     /locus_tag="PK710_21225"
     CDS             complement(1785..2441)
                     /locus_tag="PK710_21225"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOT12314.1"
                     /translation="MGRLPVIAPLLLAGIALVCCEERPKPAPSLRRADAVSASPSAAV
                     PVPAVSTKPSTPRPRRTICKGKPVDRDFPTQALGHGEAPGHESMGTQIRTGHGKWVWV
                     NLWAAWCGPCKEELPLLLSWKQKWEATADFVFVSLDDDQRQFLRFLQQQPREGLRTSF
                     WLPEGKTRAAWLADLGVESSPELPIQILVNPKGKVHCIVHGAVEPEDIPAIDAIIGKK
                     "
     gene            complement(2420..3106)
                     /locus_tag="PK710_21230"
     CDS             complement(2420..3106)
                     /locus_tag="PK710_21230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12315.1"
                     /translation="MRPHVFVGPALGTVLVLGASFWVRGQIDAAADRLANPQKAPETA
                     IASHAEAAYCTPEFKIVLQRVLNACGLVGTGSRRGCKPADVKNIASISDADFNALFLP
                     LKERGGIVMFDEGKDNLDAGANQMIEDRWLDRQGARYFFIVARGSKTGTIERNRVVSH
                     KRANSVFFHISEQSKDPNLEKQVGMLWLGKEFAQLPKEFCEWKRSRESKCDEETINRS
                     AFISWVDCRL"
     gene            complement(3103..>4009)
                     /locus_tag="PK710_21235"
     CDS             complement(3103..>4009)
                     /locus_tag="PK710_21235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12316.1"
                     /translation="IMAFIAAVRLGVFCKTCVGLYVASALLAAAGIWGMVQLRKLSFL
                     DAFSTSPPTSPAGGPPPPSPSPPHEPVRPMPPGNPGAIVLWLASLGLCVGVPAIIYVN
                     ALPDFRPLLASCGTIPQPKSKALVSLATTSPKRDMIIVADPLCPTCKGLHERLKGEGA
                     MENINASLVLMPLDSTCNWMLDRSLHPGSCILSKAVICADPRSRDALEWMYRNQEELA
                     SLGKAGDSLLRARLRTHFGEAIDTCIDAKQTAVRLNNILQYAVTNELPVSTPQLYLGD
                     LRICDEDSDLGLRFTLAQLAPEVLP"
BASE COUNT          841 a         1088 c         1174 g          906 t
ORIGIN      
        1 tcgatcctcg aggcgcggat tgcccatcat ttcccacacg tttttgggga caatggccct
       61 tcggcatgcg gtatgggcaa tgagactgtc gaaaaaaaca acacgaacca ggtcgtgtca
      121 ttggggaacg gtctgtgcga aataggcgcc cgcggcggcc gcggctccca acagcaacac
      181 gacaatggca atgatgagac ccaggccttt cttttttgcg gcaggctccg tgggtgttgg
      241 agaatccatc gagggacgag acgattcgat cgagggcggg gaaggggggg atgaggcagg
      301 ttggagagag ttttccaagg gatttgggcc cgttggcata tcctccaacg ccgacaggtt
      361 gcctacctgc aagtcctgaa gcgaagacaa gagtttgtcg ttggcacccg ttctgccaat
      421 gccataagga ttgtggattt ggatttcgga ctcccaatcc gtgatcatgc tgccatcgtg
      481 aggagaaggt gttcctgtgg gtgtcgttcg accggagggc gaagctccgc tttgttcttg
      541 cgaggtaccg agggaagtga actcgaagag ggcttgtttg atgagttggt cgatgatgtt
      601 ggcgcttgcg ggtttctgtc gctgtcgttc atcgagggct tgctgcacca aacgcgcaat
      661 atcccaagcg cccacgggtt gaccgtggtg atacaaaaat ccgtttagat cctctgccaa
      721 ggcgagggca tgcccatagc gctggttcaa gtctcgcgcc aacgagcgca ggatgatgcg
      781 ttccaagccc ggaggaacct tatgattgat ttcgggtaag gaagggatat tggcctgttg
      841 gacgagtttg acggtggcgt agtcggactc accgagaaat agttttcgtc cggccagcat
      901 ttcccacagg atgatgccca cggcgaaaat atccgcacgc gcatcgacgt cgtggcccat
      961 cgctgcctca ggggcaagat acgaaaactt ccccttgatg atgccgggtt cggacttttc
     1021 gagctgattg ttggccttgg cgagcccgaa gtccgtgatt tttatctccc cataccgggt
     1081 gatcaatacg ttcggagggg acatgtcccg atgaacgatg cccaggggcg ttcctcggcc
     1141 atctttcaag tcatgggcat acgccaatcc ctcacagatc ttggtggcga catagacggc
     1201 tgcctccaag ggaaatggtt gcccccgttt gcgaagcatc tcgatgatgg ctttgagatc
     1261 ggcaccatcc acgtactcca tcacgatgaa gtacgtgtcg tcgccaatac caatatcgaa
     1321 cacttgcacg acattggagt gactcaagtg agcgctgagc cgtgcctcat ccaaaaacat
     1381 cgacatgaac ttccgcttct ggctcaagtg cggaagcacc cgcttgatgg cgactttttt
     1441 cttgaagcct tcgattccgg cgctttccgc gcggaatact tcggccatgc cgcccgcggc
     1501 aatcttgtcg atgacccgat atcgttgctg gtggtgtccg tccggcatgt tggtcgtgcg
     1561 aggcacactc ctgcggaatg aatcgttgcc gcccatggac gttgccacct cccggtttga
     1621 acggtcaagc tatcgacgac ggatctcgct ttcatgacct tggctgagcg tctcgcgcca
     1681 tcgtgtcatg tgggcgttat tcacacctat gcatgaattc ccctgttttt ggcggatatg
     1741 ccctatccgg accccccatt ttaggcggaa cagggctggg gctcctattt tttgccaatg
     1801 atcgcgtcaa tggcggggat gtcctccggt tcgaccgctc cgtggacgat gcaatgcact
     1861 ttgcctttag gattgacaag aatctggata ggaagttcag gcgaggattc cacccccaga
     1921 tccgccaacc aagcggctcg ggtttttcct tccggcaacc aaaagctcgt ccgtaaccct
     1981 tcccgcggct gttgctgcaa gaaacgcaga aactgtcgtt gatcgtcgtc gagggagacg
     2041 aatacgaagt ctgcggtggc ttcccatttt tgtttccagc tcaacaatag cggcaattct
     2101 tctttgcagg gtccacacca tgccgcccac aaattgaccc acacccattt gccatggccg
     2161 gtgcggattt gcgttcccat cgactcgtgc ccgggagcct cgccatggcc gagcgcttgg
     2221 gtgggaaagt cgcgatcgac gggttttccc ttacaaatcg ttcgtcgcgg ccgtggtgtc
     2281 gaaggtttgg tggaaaccgc cggaaccgga acggccgcgg atggggacgc actgaccgca
     2341 tcggcgcgtc ggagcgaggg agcaggtttg gggcgctcct cacaacaaac caacgcgata
     2401 cccgccagca gaagcggcgc tataaccggc aatcgaccca tgaaatgaaa gcgctcctgt
     2461 tgatggtttc ttcgtcgcac ttgctttctc tcgaccgttt ccactcacag aattctttgg
     2521 gaagttgggc aaattctttt ccgagccaga gcattcccac ctgtttttcg aggttgggat
     2581 ccttggattg ctcgctgata tggaagaaca cggagttggc tcgtttgtga gaaaccaccc
     2641 gatttcgttc gatggttccg gttttggaac cgcgcgccac gatgaaaaaa tatcgggcac
     2701 cttgccggtc gagccatcgg tcttcgatca tttgatttgc cccggcatcg agattgtctt
     2761 tcccctcgtc gaacatcacg atgcctcccc gctctttgag cggaaggaac aaggcattga
     2821 agtccgcgtc ggaaatcgat gcgatatttt tcacatccgc gggtttgcat ccgcgacgtg
     2881 atccggtgcc taccaggccg catgcgttga gcacgcgttg cagcacgatt ttgaactccg
     2941 gggtgcaata cgcggcttcc gcgtgggaag cgatggcggt ttccggtgct ttttgaggat
     3001 ttgccaatcg atccgcggcc gcgtcgattt ggcctcgtac ccaaaaactg gctcccagaa
     3061 caagcaccgt gcccaatgcg ggacctacga aaacgtgtgg tctcaaggca gcacctccgg
     3121 agcaagctgc gccaatgtga agcgaagccc cagatccgaa tcctcatcac agattcgcag
     3181 gtccccaaga taaagctggg gcgtggatac cggaagctca ttggtaaccg cgtattgcag
     3241 gatgttgttg agacggaccg cggtctgctt ggcatcgatg catgtgtcga tggcttctcc
     3301 gaaatgcgtt cgaagccgtg cgcgcaacag ggagtcgcct gctttgccga gcgatgccag
     3361 ctcttcttgg tttcgataca tccactccag agcgtcgcgc gagcgcggat cggcgcaaat
     3421 gacggctttg gacagaatgc aactgcccgg gtgcaaagac cgatcgagca tccaattgca
     3481 tgtgctatcc agaggcatca gaaccaagga ggcgttgata ttttccatgg cgccttcccc
     3541 cttcagacgt tcgtgaagtc ctttgcacgt ggggcataag gggtcggcga cgatgatcat
     3601 gtctcgtttg ggagatgtcg tggcaagaga tacgagagct ttgctcttgg gttgtgggat
     3661 ggttccgcaa ctcgccaaca gcggtcgaaa gtccggcaac gcgttgacgt agataatggc
     3721 gggaacgccc acgcataatc ccaacgaagc gagccacaac acgatggcac ctgggtttcc
     3781 tggcggcatg ggacgaacgg gttcatgggg tggactcggt gagggaggag gagggccgcc
     3841 cgcggggctt gtcggtggag acgtcgaaaa cgcatcgaga aaggatagtt ttcgaagttg
     3901 aaccattccc cagattcccg cagcggccag caacgcagaa gccacatata gacccacgca
     3961 tgttttgcaa aacacgccga ggcgaacggc ggcgatgaaa gccatgata
//
LOCUS       DAOSOR010000539         3999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_22672_length_3999_cov_3.798174, whole genome shotgun sequence.
ACCESSION   DAOSOR010000539 DAOSOR010000000
VERSION     DAOSOR010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22672_length_3999_cov_3.798174"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1228
                     /locus_tag="PK710_21240"
     CDS             176..1228
                     /locus_tag="PK710_21240"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOT12317.1"
                     /translation="MKTDSNEHFLSRRAWLFGAGALVVGATMASRSTWLRWLPTDPSL
                     QTARFAAMGTFVDLTLAGALGSVVQNAVATIAELERRMTVFSPDSELSIINKNAALDH
                     QRVSEDLTFVLDQCNIYHRITDGAFDPTVGPLMRTWGFRGGRPTRWPNSLEIRSVLEK
                     TGMAKVDGSDGNVAFRAEGMSLDLGGIGKGYAADRAAETFQRANMVGLVNMGGDIRSV
                     GSQPDGTPWQVGVRHPYHPERLLARLELSGDRAVATSGTSEQTFELEGRSIAHILDPR
                     TGRPVSEVVSATVVADSAMKADAFATAACVLGASEAIRLFESLGGVEALLVTRHAVGS
                     RVMATQGLKVQMVASV"
     gene            1297..3993
                     /locus_tag="PK710_21245"
     CDS             1297..3993
                     /locus_tag="PK710_21245"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOT12318.1"
                     /translation="MRHRFALTSSVIAALFSVNAVQAADSLTPEAQQRGRESVPYLRN
                     NNKIAVFGAPSQAELRRAFDNATLRIRSISAHNEALVVTLEPVIRQRIDPPELRAAEA
                     YLQNLPGVKGIGPVYFEGDGLQVSTGRVWVYLPKGTGAAKAQGILSGIGLHLVDYEDA
                     FDGIAVGVPMLSFSMDALIRDLLAAGYHAVPELMKKSFPRLIPSDPYFAKQWSLRNTG
                     DNVTRAGSQAPLEGVAFADARVSEAWEWTTGVSTTLVGVLDSGTDCTHPELAGKCEQP
                     YNAITNKPSAEPPPLSQDMMAGHGTSVAGIIAAPIDGKGMVGVCPSCRIVPVRLIDSG
                     IFLTDMMILRGFKHAVDAGASVINNSWGPSVSGTYLIPVSQGELQGIKYAGQGRGGRG
                     VLVVYAAGNENADTKYLGHLRTGEPNVMGIAASNHRDRRSSYSNFGEFLDISAPTNDE
                     AITPSIISLDIVGAGDLAKDYTSAFGGTSAAAPVVSGIAALMFSLDPEVTAARVREIL
                     NETADKVDADRGFWDAQGFSVLYGHGRVNALRAILSLKGQHDPACDAPQAADDCQVHL
                     DENCDGFVDEGCSESSNVGLLCAEASECGPENHWLCPSAGKVRGACTYSCVDMPCPDG
                     SMCLVGRCAPYCNKDNPCPQSDTVCTDEGLGVCLRKCESSTDCPADEICDPVKGYCKL
                     DTDGLPGSPCTSDECKGAQGLCLSEGMGFPDGYCTHACSVNAHCENKGKCIATMNGSF
                     CYKACSFDGDCRQHYVCEQAGPRAGTCYKKCDKDSQCRGTEPGWEHIVCELSTGRCVD
                     TEEPDAGTDAAEDAATEPDGASDAQDEPDFPVEAGVADAATDATGQPEAGAHPQDGEQ
                     DDDGCSCRMVGTPTKPWGSLGVMMMAWLWSRRRRRMPVTGRV"
BASE COUNT          743 a         1081 c         1327 g          848 t
ORIGIN      
        1 ccgacgcttt cggcgctgcg ggctcgctga tcggcctcgg gctttgcgcc gcggcggttt
       61 ggctccggga ccgagccgcg agggacgccc acggcgtgcc cacaggaaga tttcaggtgc
      121 gaatccgcgc ctttgcggag gaaacgcccc ccgccccttc ttcgatgagg ttgtcatgaa
      181 aacagactca aacgagcatt tcttgtctcg tcgcgcatgg ctctttggcg ccggagcgct
      241 tgtcgtgggc gccaccatgg cctctcgtag cacctggctc cgatggcttc ctaccgatcc
      301 ttcccttcaa accgcgcgtt ttgccgccat gggcaccttt gtcgacctca cccttgccgg
      361 cgcccttggc tccgtcgtgc aaaatgccgt cgcaaccatc gccgaactcg agcggcgaat
      421 gaccgtcttt tctcccgata gcgagctgtc aatcatcaat aaaaacgccg ctttagacca
      481 ccagcgggtg tcggaagatc tgaccttcgt gctcgaccaa tgcaacatct accaccggat
      541 caccgatggc gctttcgacc ccacggtcgg gccgctgatg cgcacctggg gattccgcgg
      601 ggggcgtccg acgcgatggc ccaattcgct ggaaatccgt tcggttctcg aaaaaacggg
      661 catggcgaag gtcgatggtt ccgacggaaa cgttgctttc cgtgccgagg ggatgagcct
      721 ggatttagga gggattggca aaggttatgc cgcggaccgt gccgctgaaa cgttccaacg
      781 ggccaatatg gtcggcctcg tcaatatggg cggggatatt cgcagtgtgg gttcgcagcc
      841 cgacggtacg ccctggcagg tgggcgtgcg ccatccttat catccggagc gattgctggc
      901 acgtctggag ttgtcgggcg atcgagcggt ggccaccagc gggacttcgg agcaaacgtt
      961 cgaattggag gggcgttcga tagcgcatat tctcgatccg cgcacgggtc ggcccgtgag
     1021 cgaggtcgtg agtgcgacgg tagtggctga tagtgcgatg aaagcagatg cgtttgcgac
     1081 ggccgcttgt gtgctggggg catcggaggc aatccgtctg ttcgaatccc ttggcggggt
     1141 ggaggcgttg ttggtgacgc ggcacgcggt cggatctcgt gtgatggcta cgcaagggtt
     1201 gaaggtgcag atggtcgctt cggtgtgagc gaacgtttct ctctttcgga ggcggggcat
     1261 ctccgctatc tgcagggaag aaccctggag gttgtcatgc gccaccgatt cgctctcacc
     1321 agttccgtga ttgccgcgtt gttcagcgtc aatgccgtcc aagccgcgga ttccctgacc
     1381 ccagaggcgc agcaacgggg gagggagtcc gtcccatatc ttagaaataa taataaaatc
     1441 gcagtgttcg gtgcgccgtc ccaggcggag ctgcgtcgag cgttcgacaa cgccaccctc
     1501 cgcatccggt ccatttccgc tcacaatgaa gcgctcgtgg tgacgctcga gcctgtgata
     1561 cggcaacgta tcgatccgcc agagcttcgg gcagcggagg cgtaccttca aaatctgccg
     1621 ggggtaaaag gcatagggcc tgtctatttc gaaggggatg ggttgcaagt gtcgacgggc
     1681 cgggtgtggg tatacctgcc caaaggaacc ggagccgcca aagcccaggg aattctttcg
     1741 ggaatcgggc ttcatctcgt ggattacgaa gatgctttcg acggtattgc ggtgggtgtt
     1801 cccatgctct cgttttcgat ggatgcgttg atacgggatc tgttggccgc ggggtaccac
     1861 gcggtcccgg aactgatgaa aaagtctttt ccgcgtctta tcccttcgga tccgtacttc
     1921 gcaaagcaat ggtccctgag aaatacgggg gataacgtga cgcgggcggg ctcccaggcg
     1981 cccctcgaag gggttgcttt cgccgatgcg cgtgtgtcgg aagcgtggga atggaccacg
     2041 ggggtttcga cgacgctcgt gggggtgctc gattcgggga cggattgcac ccatccggag
     2101 ttggcgggca agtgtgagca gccctataac gccattacga acaagccttc ggccgagccg
     2161 ccccccttgt ctcaagacat gatggcgggg catggtacgt cggtggcggg aatcattgcg
     2221 gcgcccatcg atggcaaagg gatggtggga gtttgccctt cgtgccggat cgttcccgtg
     2281 cggctcatcg acagcggtat ttttctcacg gatatgatga ttctgcgggg gttcaagcat
     2341 gcggtggacg cgggagcttc ggtgatcaac aactcttggg ggccctcggt atccggaacg
     2401 tatctcattc cggtttcgca aggggagttg cagggcatca agtatgcggg gcagggcagg
     2461 gggggacgag gcgtgctggt cgtttatgcg gcgggcaatg aaaacgccga tacgaaatac
     2521 ctggggcatc ttcgaacggg cgagcccaat gtcatgggca ttgcagcctc gaatcatcgg
     2581 gaccggcgtt cctcctattc gaatttcggc gagtttttgg atatttccgc gccgaccaac
     2641 gacgaggcta tcaccccttc catcatctcg ctggacattg ttggcgcggg cgacctagcg
     2701 aaagactata cctcggcgtt tggaggcacg tccgcggcgg ctccggtggt cagtggtatt
     2761 gcggccttga tgttttccct cgatccggag gttacggcgg cccgggtcag ggagattctc
     2821 aacgaaacag ccgataaggt cgatgcggac aggggctttt gggatgccca gggcttcagt
     2881 gtgctctacg gccacgggcg ggtaaacgct cttcgggcga tactatccct aaaaggacaa
     2941 cacgatccgg cgtgcgatgc tccgcaggct gccgatgatt gccaggtgca tctcgatgaa
     3001 aactgcgatg gcttcgtgga cgaaggatgt tccgaatcat cgaacgtggg gttgctctgt
     3061 gcggaggctt ccgaatgtgg gcctgaaaac cattggttat gcccctccgc cggcaaagtg
     3121 cgcggcgcat gtacctattc ctgtgtcgat atgccttgtc ccgatgggtc gatgtgcctc
     3181 gtgggacgat gtgcgccgta ttgcaacaag gacaatccct gtccgcaatc ggataccgta
     3241 tgcaccgatg aaggtcttgg ggtttgctta cgtaagtgtg aatcgtcgac cgattgtccc
     3301 gcggacgaaa tatgcgatcc ggtgaaagga tattgcaagc tcgatacgga tggtcttccc
     3361 ggttcgcctt gcacctcgga cgagtgcaaa ggggcgcagg ggttgtgcct ttccgagggg
     3421 atgggctttc cggatgggta ttgcacgcac gcctgcagcg tgaatgccca ttgtgaaaac
     3481 aagggcaagt gcattgccac gatgaatggg tcgttttgtt acaaagcctg ctcgttcgac
     3541 ggggattgtc gtcagcatta tgtctgtgag caggcggggc cgcgcgcggg cacatgctac
     3601 aaaaaatgcg ataaagactc gcaatgccgc ggcaccgagc cgggctggga gcatatcgtc
     3661 tgtgaactct ccaccggacg atgcgtcgat acggaagagc cggatgctgg aacggatgcg
     3721 gccgaggatg cggcgaccga gccggatgga gcgtcggatg cacaggatga gcccgatttt
     3781 cccgtggaag cgggcgttgc cgacgccgct acggatgcga ccggacagcc agaagccggg
     3841 gcgcatcctc aggacggcga gcaggatgac gacggttgtt cttgccgcat ggtgggaacg
     3901 ccgacgaagc cttgggggag ccttggggta atgatgatgg cgtggctttg gtcccggcgg
     3961 cgaaggcgaa tgcccgtgac tggacgggta tgaggctgc
//
LOCUS       DAOSOR010000540         3975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_22849_length_3975_cov_4.338776, whole genome shotgun sequence.
ACCESSION   DAOSOR010000540 DAOSOR010000000
VERSION     DAOSOR010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22849_length_3975_cov_4.338776"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            727..2220
                     /locus_tag="PK710_21250"
     CDS             727..2220
                     /locus_tag="PK710_21250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12319.1"
                     /translation="MVRPIIRALLVSACFLSCFGCSSEDASGSSSPAGTGGAGNSDGS
                     VDTGSADSSGDSAGLGGSSGTGGSTGPGGAAGVGGFDGSAGLGGSAGSGGSAGSSGGT
                     QLPIEPVLSADPEVMAILDGLGDNSSAILPPLHTVGDWNSVTKEYSMHVHGPRGRDYS
                     NKAVWMPDRKRAFFCGANHGSPHRLNDAWEFDLPSHTWVMLFAPDPNNAAGVMEIVEG
                     DVLDGNGNVLETVKYVQTQRGGPTHYGHTWWGLAYEPTMKAALWMNVAIGQSAADYIE
                     KQTGSTEGIYKGPPLWAFYPYEGRWQLILSPSPWPKIIYAGAMEYVPELGGAFWYAAN
                     WNGQGMHVYQPGENVWQNLSPNNGENLYHHDQTPRTEAVMCHDRAHGVVVAQSPNHAT
                     THYDIASNTWTKVLNPGEDDTQSPRGHDAGTQMYYDPISQRCLLYDKVQPGSIWSYSV
                     ENKAWSRHTPNGPEAPTSKVISYFDEEHNVFVVNSGSTTWVYRFRIP"
     gene            2279..>3975
                     /locus_tag="PK710_21255"
     CDS             2279..>3975
                     /locus_tag="PK710_21255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12320.1"
                     /translation="MEHFSARQLASAFKLLGSVLLLTLPMACGSENEDSAGVGGSTPG
                     SGGSTGGASGSTGGASGSTGGSSGSTGGSSGSTGGSSGSTGGASGSTGGASGSSGGAS
                     GSGGSEVTPPLVDPNGPRQFLTWDGGSGPSSTQWNRHLRLPWKTTGSGDWLDAEQTPQ
                     GTVPYVSASVTEVGPVSLDVTSLVQRWLDNGENRGFYLRTDQAFAFHFVGRTASDVAN
                     RPSLEVVTDQGSFAAPPLANAQWSPTSTKAVDSRSAFKVNSGQTLAIVLFDLEGVKGK
                     VQSATLRLTCTELKYKGVLNIFEADPPVFRVGGGAEPPIEGIAAAYPLDKGIENHPSV
                     LFAADFSDIKGKYSGSCGEAEQQYDSSTESTYIRGKFIPGKTGSCGLKTQILRGTAEG
                     VPERVENALYARYYVYLESDWGSTVDGNKMPGWDNRFGWWNPAQGGYWQSTTGNGGSP
                     GTGLKVWNKSKNRWEYEGHSLRGLGGTKAGDGNYYDDLFWVGGYIYNLDQGGAFGNDV
                     GWTGTVLSKERWFCIEQYIRMNSVEGPYDAVGNGTAVKDGVYRAWVDGVLSVERTDLR
                     "
BASE COUNT          841 a         1027 c         1242 g          865 t
ORIGIN      
        1 acaagaaatg cgtaacacca tgactcccat ccggtagcct cgtccctgcc tgcacgtgag
       61 ccgggcatga gcgccgtccc tcggaatgac cccatcagcc aagactccgt aacaccgttc
      121 tgattccctc ttaccgccta agcattcaag tgctcacgct cgaagacatg cgctttggcg
      181 cgcacgagct tcaccggtct tcgacggaaa acaaccaact ctcctcatcc cattgtcctg
      241 gaacgtcctg gttactacag gaggcgcgcc acatacgaca cctgctctat cctcagccgt
      301 atttactgca acaagaagcg tgttccgttc agccccctgc aacctcgata cccgcgtttc
      361 cgccccgcga gtggtcgtcc gcatcagtgg agatagtcta aagaagaaat acttctcggg
      421 catgaagcct gaacggggct tgggatcgtt gggggatgag ctgcattgat tcggggggtt
      481 ggcggagaaa acctagaaaa aaccttggtt tctcccccct ctccgactcc taccgcggtg
      541 catgcaagga ggttcgcgac aaatcggatc cagcgcccgt cacggtatgg gacaacgctg
      601 tcgcacccca ccaatctgat aatcaagtat tttcgcaatg ttacacaatt attgagaagc
      661 tcgaactttt tttgggctgg cacgtctcct gctgtggctc gtgcgaaagg aactacagca
      721 atgcccatgg tacgtccaat cattcgcgcc cttctcgtgt ccgcttgttt tctatcctgc
      781 tttgggtgct cgtcggaaga cgcatcgggc tcttcatccc cggcgggaac tggtggcgcg
      841 gggaattccg atggttccgt ggacaccggt agcgctgata gctctgggga ttcggcgggt
      901 ttgggtggat cttcgggcac cggcggctcg acgggacctg gtggcgcggc gggcgttgga
      961 ggctttgacg gttcggcggg tttgggtggc tctgcgggct ccggcggttc ggcgggttcc
     1021 tcaggaggca cacaattgcc tatcgagccg gttctttcgg ccgacccgga ggtcatggcc
     1081 atccttgacg gccttgggga caatagttca gcgattctgc cgccccttca taccgtaggg
     1141 gattggaata gcgtcaccaa agagtacagc atgcatgtgc atggcccccg tgggcgagat
     1201 tattcgaaca aggcagtctg gatgccggac cgcaaacgag ccttcttttg cggcgccaat
     1261 catggatcac cgcaccgttt gaacgatgca tgggagtttg acttgccttc ccatacctgg
     1321 gtgatgcttt tcgctcccga cccgaacaac gccgctggag tgatggagat cgtggaaggc
     1381 gacgtgctcg acggcaacgg caatgtgttg gaaacggtaa aatacgtaca aacccaacgc
     1441 ggaggaccta cccactacgg tcatacatgg tggggattgg cgtatgagcc cacgatgaaa
     1501 gcggcgttgt ggatgaacgt cgccattggt caatctgccg ctgattacat cgaaaagcag
     1561 actggctcga cggagggaat ctacaaggga ccgcctttat gggcctttta tccctatgaa
     1621 ggtcgatggc aactcatttt atctcccagt ccgtggccga agattattta cgccggcgcg
     1681 atggaatacg tgccggagct ggggggggcg ttctggtatg cggcgaattg gaatggccag
     1741 ggcatgcatg tttaccagcc gggcgaaaat gtgtggcaaa acctgagccc caacaacggc
     1801 gagaacttgt accaccacga tcagacaccg cgcaccgaag cggtcatgtg tcatgatcgc
     1861 gcccacggtg tcgttgtggc gcaatcgccc aatcacgcca cgacacacta cgatattgcg
     1921 tccaacacct ggaccaaggt gttgaatcca ggcgaggacg atacccaaag tccgcggggg
     1981 cacgatgccg gcacgcagat gtattacgat cctattagcc agcggtgcct gctttacgat
     2041 aaagtgcagc ctggaagtat ctggtcttac tcggtcgaaa acaaggcgtg gtcacgtcat
     2101 acgccgaatg gcccggaggc tcccacttcg aaagtcatca gctatttcga cgaggagcat
     2161 aacgtattcg tggtgaattc cggtagcacg acgtgggtat atcgttttcg gatcccttga
     2221 ccgagaccgc tgaagcactt tcggatggtt tcaacttcgt tttgtctgga ggagagtcat
     2281 ggaacatttc tcggcacgtc aattggcgtc ggctttcaaa cttttgggaa gcgttctttt
     2341 gctgaccctg ccgatggctt gtgggtcgga gaatgaagac agtgcgggtg tcggaggctc
     2401 gacgcccggt tcgggtggtt cgaccggtgg cgcgagcggt tcgaccggcg gcgcgagtgg
     2461 ctctactggt ggttcgagcg gctcgaccgg tggttcgagc ggctcgaccg gtggttcgag
     2521 cggctcgacc ggtggcgcga gtggctctac cggcggcgcg agcggttcga gcggtggcgc
     2581 gagcggttca ggtggttccg aggtaacccc tccccttgtt gacccgaatg gcccacgcca
     2641 gttcctcacg tgggatgggg gtagtggacc gagcagcacc caatggaatc gtcatctgcg
     2701 cttgccgtgg aagactaccg gttcagggga ttggctcgac gccgagcaga ctcctcaggg
     2761 cacggttcct tacgtttcgg caagtgttac cgaggttggc ccggtgagtc tcgacgtgac
     2821 ttctcttgtg caacgctggc tcgacaacgg cgagaaccgg ggattttacc tccgaacgga
     2881 ccaggccttt gcgtttcatt tcgtggggcg aacggcaagc gatgtcgcca atcgaccgtc
     2941 tctcgaagtg gtgacggatc agggttcgtt tgcggcgcca ccgcttgcga atgcgcagtg
     3001 gtccccaaca tctacgaaag cagtcgacag tcgcagcgcg ttcaaggtca acagcgggca
     3061 aacgttggct atcgtgcttt tcgatttgga aggggtgaag ggaaaggtgc agagtgcaac
     3121 gcttcgtttg acgtgtacgg aattgaagta taagggagtc ttgaatattt ttgaagcgga
     3181 tccaccggta tttcgagtgg gaggtggggc ggaaccgccg atcgagggga ttgccgctgc
     3241 ctatccgttg gacaagggga tagagaatca cccaagtgta ttgttcgcgg cagatttttc
     3301 ggatattaag ggcaaatatt cgggaagttg tggagaggcg gagcagcaat acgactccag
     3361 cacggaatcc acgtatatcc gtgggaaatt tatccctgga aaaacgggga gttgcggatt
     3421 gaagacgcag attttgcgag gaactgcgga gggtgtgccg gaaagggtcg aaaacgcgct
     3481 ctatgcaaga tactatgtgt atctcgaaag cgactggggc tcgacggtag atggcaataa
     3541 aatgccggga tgggacaatc gttttgggtg gtggaatccg gcgcaggggg ggtattggca
     3601 atcgacgacg ggcaacggtg ggtcaccggg caccgggttg aaagtctgga acaagagcaa
     3661 aaatcgctgg gaatatgagg gacattcgct acggggtctc ggcggaacca aggcgggaga
     3721 tggcaactat tatgatgacc tattttgggt gggcgggtac atctacaatc tggaccaagg
     3781 gggcgctttc ggcaacgacg tgggctggac gggtacggtt ctatccaaag agagatggtt
     3841 ttgcatcgag caatacatac gcatgaattc agtggaaggc ccctatgatg ccgtgggcaa
     3901 cggaaccgcc gtgaaagacg gagtctatcg tgcgtgggtg gatggcgtac tatccgtcga
     3961 gcgcaccgat ctgcg
//
LOCUS       DAOSOR010000541         3970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_22878_length_3970_cov_3.996424, whole genome shotgun sequence.
ACCESSION   DAOSOR010000541 DAOSOR010000000
VERSION     DAOSOR010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22878_length_3970_cov_3.996424"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="PK710_21260"
     CDS             complement(<1..1333)
                     /locus_tag="PK710_21260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12321.1"
                     /translation="MTDSRFPPTGTLSISVRLTPSPTPGEPRVDALFDDYASTRRHLF
                     VRHNEGATTNAGDCPLQIVFQDVNSYAAGWTAHCSCDAWHDISVSWNHTTRKASVTID
                     GEVVGMADLPAGYVPDEQRFTFSGRGDVFRSVDVWENDDATGAAMASFRFPEGTDDLT
                     SAEGGTHRMRLTGNFRWVTTEKGEPAIALDHESPRGRMTMIGSSELIEGFAKLQSRAQ
                     DFATAFNHGDTSRSDQPLVIRTIALAWLLTDEEPYREALFHAADAILATSPDSVDDMV
                     GSAIADSIGVIYDTLHKDVGTRTHAPSGTTYRVAIAEFVKASLRHWASYICGGEFDSQ
                     WKCPAGETIYVSGSHAPNNSGKFGSAVLALVDDFPELENVAVRLLANLKAVQAFRDYA
                     LADGGHYFGFAYGAGYSELRSTMIWHTATDANVLWPYMANLPLHYIYGLRAD"
     gene            1833..2222
                     /locus_tag="PK710_21265"
     CDS             1833..2222
                     /locus_tag="PK710_21265"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOT12322.1"
                     /translation="MGSKATKAVAVDDCGRVQGRSIEPGAPGIAQQARRMLEALSYRV
                     ETNGEASIVATGHGRELVAVATKKWTEISCHAPNAFDVMNRPGMLIDGGGRDTKGNRV
                     RSDGSVVDFVMNDKCATGTGRFFVLLG"
     gene            complement(2319..2447)
                     /locus_tag="PK710_21270"
     CDS             complement(2319..2447)
                     /locus_tag="PK710_21270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12323.1"
                     /translation="MVTLFDFLTFTKGWTYVVAGLLLVSFIPFYLFLTEREKRSGP"
     gene            complement(2464..3630)
                     /gene="nrfD"
                     /locus_tag="PK710_21275"
     CDS             complement(2464..3630)
                     /gene="nrfD"
                     /locus_tag="PK710_21275"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HOT12324.1"
                     /translation="MSEAVQTRGKLFTPFNLIAGVILLVGAGILLLRFTQGLQATTNL
                     THYYPWGIWIGFDVMSGVALAAGGFVTSSAVYLFGMKEYKPIVRPAILTGFLGYFLVV
                     LGLIVDLGKPWHLPYPFLVQAGPTSALFEVSLCVALYLTTLFVETTPAVFEWLGWKRW
                     RKVVGAMTIALTVFGLILSTLHQSTLGAMYAITPTKLHPLWYSPYIPVHFFVSAVAAG
                     LSMVIVEGMFSHKAFHHQVQISNEQFERINLGLGKAGAVALAIYTAIKLLSLSLDDKW
                     HLLGTSWGAWYLVELVGFVLLPCFLFTVGYRESKVLFVRIAAVITVIGIILNRFNVSF
                     IAFNYDLPSEHRYVPHWMEIWVSVALVTFGVVMFRWIANRMPIMYEHPDWKDEH"
     gene            complement(3627..>3970)
                     /locus_tag="PK710_21280"
     CDS             complement(3627..>3970)
                     /locus_tag="PK710_21280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12325.1"
                     /translation="HESLPSIGTSYLSVVPLVVTIYPGLLMAFYAFSKRKDKLAQQEL
                     EEAVRLALEKADEDTKEKLKQAADKSAKDKEKAINAAVKKALQEAERKAAAEKAAAAA
                     TDGADPKETKS"
BASE COUNT          925 a         1065 c         1167 g          813 t
ORIGIN      
        1 gatccgcgcg aaggccatat atgtagtgaa ggggcagatt tgccatatac ggccacagca
       61 cgttggcgtc ggttgccgta tgccaaatca tcgtacttcg gagttcggaa tatcctgccc
      121 cataggcaaa tccgaagtaa tgtcccccat ccgccaacgc ataatcgcgg aaagcctgca
      181 ccgccttcaa gttcgcaagc agccggaccg caacgttttc gagttcggga aaatcatcga
      241 caagcgccaa caccgcactt ccgaacttgc cggaattgtt cggggcatgc gagcccgaga
      301 cataaatggt ttcgcccgcg gggcacttcc actgggagtc gaattcacca ccgcagatat
      361 aggaagccca gtgacgaagg gacgccttga cgaattcggc gatggcgaca cgataggttg
      421 ttccgctcgg tgcatgggtg cgtgtcccaa cgtccttgtg caacgtgtca tagatgactc
      481 cgatgctatc tgcgatggcg gaaccaacca tatcatccac ggagtctggg gaagttgcga
      541 gaatggcgtc ggcagcgtgg aaaagagctt cacgataggg ctcttcgtcg gtcagaagcc
      601 acgccaaagc gatcgtgcga atcacgagcg gttgatcgct gcgcgatgta tctccgtgat
      661 tgaaagcagt tgcaaaatcc tgggctcggc tttggagctt ggcaaatccc tcgatgagtt
      721 cgctcgaacc gatcatcgtc atacgccccc gtggcgattc gtgatcgaga gcgatggcag
      781 gttcgccctt ttccgtggta acccaacgga agttgccagt aagccgcatc cgatgggtgc
      841 ctccctcggc agaagtaagg tcgtccgttc cctcgggaaa gcgaaaactc gccatggcgg
      901 ctcccgtcgc gtcgtcgttt tcccaaacgt cgacacttcg aaacacatcc ccacgccccg
      961 aaaacgtgaa acgttgctcg tcgggaacat agcctgctgg caagtccgcc attcccacaa
     1021 cttcaccatc gatagtaaca ctcgccttgc gcgtggtgtg attccaggaa accgaaatat
     1081 cgtgccatgc atcgcacgaa caatgcgctg tccatcccgc tgcataggaa ttgacatcct
     1141 gaaagacgat ttgcaaagga caatcccctg cgttcgtggt ggcgccttcg ttgtgtcgta
     1201 cgaaaagatg ccgtcgtgtg ctcgcatagt cgtcgaaaag cgcatccaca cggggctcgc
     1261 ctggcgtggg ggaaggcgtg agtcggacgg atatcgagag ggttcctgtc ggcgggaacc
     1321 tggaatccgt caaaggaatt ggatcgaaaa ccgtggccct gagtttttcg acgacctaag
     1381 gactcacaag aatgcggggg cgtggattga gcttgaaagg gagtgcggcg cgcgccgtcg
     1441 acccaagccc caatcccaac accgcgaata ggagagccgc ccgcttcatg actggccatg
     1501 atacgagact gtcatcgcag ggatcaaccg tcttggggcg tgcggagagg ctgttcaacc
     1561 tgaatcatcc ccttattttt ccagagtaag tctcccgtcg cagcgctacc ctgtgtaggc
     1621 gcgaatgctc atccgtcgaa gcctcaacgt tcgccgggag atgataaaaa cttgtaaata
     1681 caaatagtta caacgattcg ttcctgcacg gccaccattc gtatcacccc tgttcggtcg
     1741 accaaatcaa cctggcggat cgtctcgcta aagaagatcc atccgaacac catggctcga
     1801 cggagatcac attgccatgc actggaaacg acgtggggtc gaaggcgacc aaggcggtag
     1861 cggtggacga ctgcggtcgt gtgcagggtc gttcgatcga accgggggct ccaggtattg
     1921 cacagcaagc gcggcgcatg ctcgaggcat tgtcctatcg cgtggaaacc aatggcgaag
     1981 catcgatcgt ggcaacgggc catggtcgcg agctggtagc ggtggccacg aaaaaatgga
     2041 cggagatttc atgtcatgcg ccgaatgcat ttgatgtcat gaaccggccg ggaatgttga
     2101 tcgacggggg tggcagggac acgaaaggga accgagtgcg ttcggatggt tcggtggtgg
     2161 atttcgtgat gaacgacaag tgcgcgacgg gaacagggcg ttttttcgtc ttgttgggat
     2221 aaccgaggtt cgttagcgcc gaagggcgct gccgcggaat cctgaggtct tcgatcagaa
     2281 tgcgttgcgc gaagagcgct tgcattttca tcaagcgatc aagggccgct tcgtttttct
     2341 cgttccgtga gaaatagata gaaggggatg aaactgacga gcaacaggcc cgccaccacg
     2401 tatgtccagc ccttcgtgaa cgtcagaaaa tcgaataacg ttaccatggc gtcctccgta
     2461 ggctcaatgt tcgtctttcc aatcgggatg ttcgtacatg atgggcatgc ggttcgcgat
     2521 ccatcggaac atcacgacac cgaacgtgac caacgccacg ctcacccaaa tctccatcca
     2581 atgaggaacg taacggtgtt cggagggcag gtcgtaattg aacgcgatga acgagacgtt
     2641 gaagcgattg aggatgatac caatcaccgt gatgaccgcc gcgattcgga cgaatagcac
     2701 cttggattcg cggtagccta ccgtgaaaag gaaacaaggg agcaagacga aaccgacgag
     2761 ttccacgaga taccaggctc cccaggacgt tccgagcaga tgccatttgt cgtcgaggga
     2821 aagcgaaagg agtttgatgg ccgtgtagat ggcaagcgcg acggcaccgg cttttcccag
     2881 gcccaggttg attcgctcga actgttcgtt ggagatctgg acttgatggt ggaacgcctt
     2941 gtgagaaaac atgccttcca cgatcaccat gctcaacccg gcggccacgg cggaaacgaa
     3001 aaagtgaacg ggaatatacg gggaatacca cagaggatgc agcttggtgg gcgtgatggc
     3061 atacatcgca cccaacgtgg attgatgcag cgtggataag atgagaccga agaccgttag
     3121 agcaatggtc atggctccca cgacttttcg ccagcgcttc catcccaacc attcgaagac
     3181 cgcgggcgtt gtctccacga atagggttgt caggtacaac gcgacacaga gggatacttc
     3241 gaacagggcg gaggtaggtc cggcctggac gagaaatgga tagggaagat gccacggttt
     3301 tcccaagtcc acgatgagtc cgagcacgac gagaaagtag ccgagaaagc ctgtgaggat
     3361 ggcagggcga acgatcggct tgtattcctt catgccgaat agatacacgg ccgaggaagt
     3421 cacgaagcca ccggcggcga gagccacacc gctcatgaca tcgaagccga tccatattcc
     3481 ccaggggtaa tagtgggtaa ggttggtggt ggcctgcaag ccttgggtga aacgcaagag
     3541 caggataccc gcacccacca acagaatgac gcctgcaatg agattgaagg gcgtgaagag
     3601 cttgcctcgg gtttggactg cctcgctcat gacttggtct ccttggggtc cgcaccgtcg
     3661 gtggcggcgg cggcggcctt ctccgccgct gcctttcgtt cggcttcctg cagcgctttt
     3721 ttcaccgccg cgttaatggc tttctcctta tctttcgcgg acttatctgc cgcttgcttg
     3781 agtttttcct tggtatcctc gtcggctttc tcgagggcga gccgcaccgc ttcttccaac
     3841 tcctgttggg caagcttgtc cttgcgtttg ctaaatgcgt agaaggccat gagcagcccg
     3901 ggatagatgg tgaccaccaa aggcacgaca gatagatagc tcgtaccgat ggaaggcagc
     3961 gattcgtgcg
//
LOCUS       DAOSOR010000542         3969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_22883_length_3969_cov_3.867910, whole genome shotgun sequence.
ACCESSION   DAOSOR010000542 DAOSOR010000000
VERSION     DAOSOR010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22883_length_3969_cov_3.867910"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PK710_21285"
     CDS             <1..370
                     /locus_tag="PK710_21285"
                     /inference="COORDINATES: protein motif:HMM:NF019982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT12326.1"
                     /translation="SFVTVGVFWLLLGRRAMMLEPMLQQYMTDIFKPENFPNYLRLFQ
                     ELDAHSAFHHLPEIDVPALVVSGRFDVLTPPYQSREIARRLAHAERLHVRNGSHFVLI
                     ERPEQVVPRVERFLRERVKW"
     gene            687..1067
                     /locus_tag="PK710_21290"
     CDS             687..1067
                     /locus_tag="PK710_21290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT12327.1"
                     /translation="MAKILIVDDDPDVVDACRMFLEREHYDVASAFSREEGMKAVREI
                     DPDLIILDVIMEQPDDGFAMAQQLRRDGFLKPILMLTSIGRVTGLTYGKHADLLPVDA
                     FVDKPVEPAILVQKVAALLGGKEG"
     gene            1072..1602
                     /locus_tag="PK710_21295"
     CDS             1072..1602
                     /locus_tag="PK710_21295"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOT12328.1"
                     /translation="MLLTERQRICEDVTAWTHELGSDRSAMLPILQLIQAKYREVSPY
                     SMQVVADQLGIHPVEVYSVVTFFAFLREGTHGKFVIRLCRTITCDMAGKDRIAQQLRN
                     DLGIDFGQTTPDGQFSLEWTSCMGMCDQGPALLVNDQVFTRVTSDMVHDIVEACRRSF
                     GPLAFHSREVKTEVDQ"
     gene            1599..2792
                     /locus_tag="PK710_21300"
     CDS             1599..2792
                     /locus_tag="PK710_21300"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOT12329.1"
                     /translation="MTSSIMPGLVTFKTIVPNEGLQAALARSRSDVIHEISASKLKGR
                     GGAGFPTGVKWNLAAAACGEPKYIVCNADEGEPGTFKDRVILSEIADIVFEGMTIAAY
                     AIGATKGILYLRGEYTYLRASLEDVLRKRREANLLGDKILGSDLSFDIDIHMGAGAYV
                     CGEETALIESLEGHRGEPRNRPPFPVDTGFQGKPTVVNNVETFVCAAAILGKGAAWFS
                     AIGTEKSSGPKLFSVSGDCDRPGVYEFPLGVTIEQLLDVVGGNNAKAVQVGGASGQCI
                     PAGQFDRAICFEDVSTGGSVIVFGPQRDMLHIAKNFMEFFVEESCGQCTPCREGNAKL
                     LEGIERLEKGQCSMAYLNELCRLGEVMQLSSKCGLGQSSPNAFLSIVKHFREELMGRP
                     HPATR"
     gene            2804..>3969
                     /locus_tag="PK710_21305"
     CDS             2804..>3969
                     /locus_tag="PK710_21305"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOT12330.1"
                     /translation="MSEFKPAIPTRAPVSQRGHFVPPASNPDAIGAKVRVNIDGMDIQ
                     VPIGTTILEAARQLGIRIPTLCHHPDLCVAGVCRICVVEVEGQRTLQASCAYPITAPI
                     TVKTHTAKVRQSRRAILDLMLSKHVGECYSCFRNLNCELQTLCKEYGVDYYRFGHPTE
                     PMFEVDKSSYSVRRDMNKCILCRRCVRTCIDLQEVGVLEAIHRSDKTTIATFMQRPLA
                     EVICINCGQCINRCPTAALGAADPTDELWRDINDPTKHVVIQTAPAPRSGIGECFGLP
                     AGVPLTFQMNTALRTIGFDKVFDTNFTADLTIIEEGTELLLRLYKALVLEQAVALPQF
                     TSCSPGWIKYIEHFYPEMLPHVSSAKSPQQMFGSLIKTYYAKLHGIDPANIVTVA"
BASE COUNT          871 a         1065 c         1136 g          897 t
ORIGIN      
        1 gtccttcgtc accgtggggg tgttttggtt gttgttggga cgtcgcgcga tgatgctcga
       61 accgatgcta cagcaataca tgacagatat tttcaagccg gagaacttcc cgaattactt
      121 gagattattt caagaactcg acgcgcattc ggcctttcat catttgccgg agatcgatgt
      181 tccggcgctt gtcgtcagtg gacgattcga cgtgttgacg ccgccgtatc aatcgcgtga
      241 gattgcgcga cgacttgccc atgcggagcg gttgcatgtg cgcaatggga gtcacttcgt
      301 gctgattgaa agaccggagc aggtcgtgcc cagggtggag cgatttctcc gcgaacgcgt
      361 gaagtggtga atgctagact ctgtcataag aacagtgaca catcagattc aggaatcctt
      421 tacgacgaag tcggcgtaac gtcgataaac gaagaataat ccctataatc ataggatatt
      481 tcttctcggt ggagtgcgtc ggcagggctg tgaacggagg aagggaaaga acgatagatc
      541 gctattcttc tcatccatac ttgcgagttg ggcgaaatct tagtagccca tgaacgagca
      601 agatggtggc cttgccgcaa aacgacaaat gccgttttgg cggggaattc gggatagggg
      661 ttcggaaccg ggaataggag acgcttatgg cgaagatcct catcgttgac gacgatccgg
      721 atgttgtgga cgcgtgtcgg atgtttctcg aacgagagca ttacgacgta gcttctgcct
      781 ttagccgcga ggagggaatg aaagcagttc gggagattga tccggacttg atcatcttag
      841 atgtgatcat ggagcagccc gatgacgggt tcgcgatggc gcagcaactg cgtcgtgatg
      901 ggttcctcaa gccgattctg atgctcacga gcatcggccg ggtgacaggc ttgacgtacg
      961 gaaaacacgc cgatttattg cccgtggacg cttttgtcga caagcctgtc gaaccggcga
     1021 ttctcgtgca gaaggttgcg gccctgctgg gcggaaagga agggtagtcg catgctgctc
     1081 acggagcgcc agcgcatctg cgaggatgtg acggcttgga cccatgaact tggcagtgac
     1141 cgctcggcga tgctgcctat cctgcaactg attcaggcaa agtatcgaga ggtgtcgccg
     1201 tactcgatgc aggtggtggc tgaccaactc ggcattcacc ccgtcgaggt gtatagcgtc
     1261 gttaccttct ttgcctttct tcgcgaagga acccacggaa agttcgtcat ccgcttgtgt
     1321 cgaaccatca cctgcgacat ggccggaaaa gaccgtatcg cgcaacaact tcgcaacgat
     1381 cttggaatcg acttcgggca aaccacaccc gatggccaat tctccctcga atggacgtcg
     1441 tgcatgggaa tgtgcgatca agggcccgcc ttgctcgtca acgatcaggt attcacgagg
     1501 gtaacctccg atatggtcca cgatatcgtc gaagcctgcc gtcgaagctt cggtccgctt
     1561 gcctttcata gtcgcgaagt gaaaacggag gtggatcaat gacctcttcg atcatgccgg
     1621 ggctcgtcac gttcaagacg attgtgccca acgagggact gcaggccgcg cttgcacgaa
     1681 gtcgttccga tgtcatccac gagatttccg cgtcgaagct caaggggcga ggcggtgctg
     1741 ggtttccgac cggggtcaag tggaatcttg ctgccgcggc ttgcggtgaa ccgaaataca
     1801 tcgtgtgcaa cgccgacgag ggcgaacccg gaaccttcaa ggatcgcgtc attctctcgg
     1861 agattgccga tatcgttttc gagggaatga cgatcgcggc ctacgcgatc ggggctacga
     1921 aaggcatatt gtacttgcgc ggagagtaca cgtacctacg tgcttcgctc gaggatgtct
     1981 tgcgaaaacg ccgtgaggca aacttgctcg gggacaaaat tcttggctcc gatttgtcgt
     2041 tcgacatcga tattcacatg ggggcgggtg cttatgtatg cggcgaggaa acggccttga
     2101 tcgaatcgct cgaaggacat cgaggagagc cacgaaatcg tccgcctttc cccgtggaca
     2161 ccggatttca aggcaaaccc acggtcgtca acaatgtgga aactttcgtg tgtgcagccg
     2221 cgattcttgg taagggagcg gcatggtttt ccgccatcgg tacggaaaag tcttcgggac
     2281 caaaactgtt ctccgtatcc ggcgattgtg atcgccctgg cgtttatgag tttcctctcg
     2341 gcgtgaccat cgagcaattg ctcgatgtgg tgggaggaaa caacgccaaa gcggttcagg
     2401 tgggcggcgc atcgggacaa tgcatccccg cagggcaatt cgatcgagcc atctgtttcg
     2461 aggatgtgtc cacgggaggt tccgtcattg tattcggccc ccaacgggac atgcttcaca
     2521 ttgcaaaaaa cttcatggag ttttttgtcg aggagtcttg tgggcaatgt acgccgtgtc
     2581 gtgaaggcaa tgccaaactc ctcgaaggca tcgagcgtct cgaaaaaggt caatgctcga
     2641 tggcctatct caacgagttg tgtcgactcg gtgaagtcat gcagctttcc tccaagtgcg
     2701 gccttgggca atccagtccc aatgctttcc tgtcaatcgt caagcacttc cgcgaagaac
     2761 tgatgggacg gccccatccg gcgacgaggt aaaggagcgc cccatgtcgg aattcaaacc
     2821 agcaatccct actcgcgcgc ccgtcagtca gcgcggtcac ttcgtgccac ccgcctccaa
     2881 tcccgatgcc atcggagcca aggtgcgcgt caacatcgat ggaatggaca ttcaagtgcc
     2941 gatcggcacg accatcctcg aagcggctcg gcaactgggc attcgtattc ccaccctctg
     3001 tcatcacccc gatctttgtg tggctggcgt ttgccgaatc tgcgtggtgg aggtcgaggg
     3061 acaacgaacg cttcaagctt cttgtgccta tcccatcacg gcacccatta cggtgaaaac
     3121 gcataccgcc aaagtgcgcc agtcgcgtcg cgccatcctt gacctcatgt tgtccaagca
     3181 cgtgggcgaa tgctactcgt gtttccgaaa cctgaactgc gagctgcaga cactgtgcaa
     3241 ggaatatggc gtcgattact accgtttcgg ccatcccacc gaacccatgt tcgaagtgga
     3301 taaatcgtcg tactcggttc gtcgagacat gaataaatgc attctatgtc gacgctgcgt
     3361 gcgtacttgt atcgacttgc aagaggtggg cgtgctcgag gccatccatc ggtcggacaa
     3421 gacgaccatc gccacgttca tgcagcgccc gttggccgag gtgatttgta tcaactgtgg
     3481 gcagtgcatc aatcgttgcc cgaccgcggc cctgggcgcc gcggatccca ccgatgaact
     3541 ctggcgcgac atcaacgatc ccaccaagca tgtcgtcatt caaacggcac cagctcctcg
     3601 ttcgggaatt ggcgaatgct ttggattgcc cgcgggggtg ccgttgacgt tccaaatgaa
     3661 tactgccctt cgcaccattg ggttcgacaa ggtatttgac accaacttca ccgcagacct
     3721 gacgatcatc gaggagggaa cggagcttct ccttcgtttg tacaaagctc tcgtgctcga
     3781 gcaagccgtg gctctgccgc aatttacctc ctgctcgccc ggatggatca aatacatcga
     3841 acacttctat ccggaaatgt tgccacacgt ctcatcggcc aagagcccgc agcagatgtt
     3901 cggctcgctc atcaaaacgt attatgcgaa gttgcatggc atcgatccgg ccaacatcgt
     3961 gacggtggc
//
LOCUS       DAOSOR010000543         3969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_22884_length_3969_cov_3.798927, whole genome shotgun sequence.
ACCESSION   DAOSOR010000543 DAOSOR010000000
VERSION     DAOSOR010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22884_length_3969_cov_3.798927"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PK710_21310"
     CDS             complement(<1..463)
                     /locus_tag="PK710_21310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12331.1"
                     /translation="MPAAIPIPAFRRALCLGSARLVSSAPWRFAKVIGIILAISFTFA
                     IIYLSGHGLGHQVHPIVARTAVLIAWLGGAVIALWNAGDRAKADHLDGIDALARTHGI
                     DPHALIISRFLAVALRVTALVMLTCLPVVIASIAVSPSFSDALKRTLSLLPL"
     gene            620..2389
                     /locus_tag="PK710_21315"
     CDS             620..2389
                     /locus_tag="PK710_21315"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HOT12332.1"
                     /translation="MTAILGLSAYYHDASAALVIDGRIVASAQEERFSRIKHDPSFPN
                     QAISYCLKQGGLASSDLEYVVFYEKPYRKLERLIDTWLAFAPRGYRAFLRAVPSWMGV
                     KLHLSREISRSLGSVPQHRLTFVQHHESHAASAFFPSPFTRAALLTLDGVGEWATATI
                     GVGDGNRLEILFEQRFPHSLGLLYAAFTHLAGFRINSGEYKLMGLAPYGQPRFVPTIM
                     DNLIDLRADGSFRLDLSYFDFAHGSTMTSKKFHRLFEGPARQPEAPITQREKDLAASI
                     QAVTQEIVLRCARHLHQHTGEKNVCLAGGVALNCVANGWLRSHGPFERVWVQPASGDA
                     GGALGAALFVWHQLLSQPRIVHGGDTMQGALLGPCFSDDELEAFLQSKGLRFQRYPDD
                     RSLCERVADLLEQQAVIGWFQGRMEYGPRALGNRSIVADPRSQQHRDTLNLKIKFRES
                     FRPFAPSVLAERAHEWFDVPIGYEDPYMLFVVNVRGDVTPLPAVTHIDGTARVQTVDG
                     RHGRYRLLLEAFAERTGCPVLVNTSFNVRGEPIVCTPADAYGCFLRTNLDVLVLGRCV
                     VVRDEQVDVPRHTVAPTGQFPLD"
     gene            complement(2498..3013)
                     /locus_tag="PK710_21320"
     CDS             complement(2498..3013)
                     /locus_tag="PK710_21320"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT12333.1"
                     /translation="MANPQVFFDITIGGAPAGRVTFELFADVVPKTAENFRALCTGEK
                     GMGRSGKPLHFKGSSFHRIIPNFMCQGGDFTRGNGTGGESIYGEKFADENFKLKHSGP
                     GTLSMANAGPNTNGSQFFICTADTSWLDGKHVVFGRVIDGMQVVQQMEAVGSRNGATS
                     KPVVIADCGQL"
     gene            complement(3044..3340)
                     /locus_tag="PK710_21325"
     CDS             complement(3044..3340)
                     /locus_tag="PK710_21325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12334.1"
                     /translation="MTRTQYLTVHPLISGDERITLAIDWVTTGASCMLPAVSVQHARM
                     LLQPTIVVERPPEGPARGKWEGAPWMIIALVVVAVLGTSAYWLMQLRKPRNPQP"
     gene            3426..>3969
                     /locus_tag="PK710_21330"
     CDS             3426..>3969
                     /locus_tag="PK710_21330"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOT12335.1"
                     /translation="MLCSSIPLVNVANAMRLAFVFAPYHHKRFEENISVVDEDFGLFP
                     PINVAYAAAIAERAGHTVRIFDANADIRLGRASEDQVLTQLQEFRPNAVGLYFSTYMF
                     RDTLAWARRIKARLGCVILAGGVNCSVYPRETLEHPEIDFIVVGHADKSLPALLDALD
                     GGGTIDSIAGVGCKVEGELVV"
BASE COUNT          781 a          984 c         1281 g          923 t
ORIGIN      
        1 cgaggggaag cagtgacaag gtgcgtttca gcgcatcgga gaaggaaggg gaaacggcaa
       61 tggaagcgat cacgacaggc aaacaggtga gcatgacgag tgcggtcacg cgcaaagcga
      121 cggcgaggaa tctacttatg atgagggcat gagggtcgat gccgtgggtt cgggcgagtg
      181 catcgatacc atcgaggtgg tctgctttgg cacggtcgcc cgcgttccac agggcgatga
      241 cggcaccacc gagccaggcg atcagcacgg cggttcgggc tacgatggga tggacttggt
      301 ggccgagccc atggcctgat aggtagatga tggcgaaggt gaaggaaatg gccagaatga
      361 tgccgatgac tttggcaaag cgccagggcg cggaggacac cagacgcgcg gagccaaggc
      421 atagggcgcg tcgaaaggcg ggaatgggta tggcggcggg catgggcggg tagcattcaa
      481 caccggcgca ggtcgaactg ccaaggaaca aaaacgttcg tgtgcgaagg agggcgagga
      541 aagggtgaac aaggaaggtt tgcgaatgat tcggtttctc gacgcaatat ggctcgatga
      601 ttaaggttaa ccctcgccca tgacggccat tttgggtttg tcggcatatt atcacgatgc
      661 atcggcggct ttggtcatcg acggccggat tgtggcatcg gctcaggaag agcgtttttc
      721 aaggatcaaa catgatccat cgtttccgaa tcaggccatt tcgtattgtc tgaagcaagg
      781 gggcctggcg tcatccgact tggaatatgt tgttttctat gaaaaacctt atcgaaagct
      841 cgagcggctg atcgacacct ggctcgcgtt tgcgccgcgc gggtatcggg catttctgcg
      901 ggccgttcca tcgtggatgg gcgtcaagct gcatctatct cgggagatat ctcgaagtct
      961 tgggagtgtc ccccagcatc gtcttacgtt cgtgcagcac cacgaatcac atgcggccag
     1021 tgcgtttttt ccttcgcctt ttacgcgcgc ggctttgttg acgctcgatg gggttggcga
     1081 atgggccacc gcgacgatcg gagttggaga cggcaatcgg ttggagattt tgttcgagca
     1141 gcggtttccg cattcgttgg ggttgctcta tgcagcgttc acccatttgg cgggatttcg
     1201 catcaactct ggcgaataca aattgatggg attggcgcct tatggtcagc cgcgatttgt
     1261 gccgaccatc atggataatc tcatcgatct tcgagcggat ggatcatttc gcctcgatct
     1321 ttcgtatttc gactttgcgc atggttcgac gatgacatcg aagaaattcc atcgattgtt
     1381 cgaggggcca gcccgacagc cggaggcacc gatcacgcag cgggaaaagg atctggcagc
     1441 atcgattcaa gcggtgaccc aagagatcgt gcttcgatgt gcgcgtcatc ttcaccagca
     1501 cacgggtgag aaaaacgtgt gtcttgcggg cggtgttgca ttgaactgcg tggccaacgg
     1561 ttggttacgt tctcatggtc catttgaacg cgtatgggtt cagccggctt cgggtgacgc
     1621 cgggggcgcg cttggtgccg cgctatttgt ttggcaccag cttctttcgc agcctcgaat
     1681 cgtgcatggt ggcgatacga tgcaaggggc gttgttgggt ccctgcttca gcgatgatga
     1741 actcgaagct tttttgcaat ccaaagggct gaggtttcag cggtaccctg acgatcgcag
     1801 tctatgtgag cgggtcgcgg accttctcga gcaacaagcg gtgattggat ggttccaagg
     1861 cagaatggaa tacggcccgc gagcgctcgg caatcgcagc attgttgcgg acccacgaag
     1921 ccagcagcac cgcgatacgc tcaacctgaa aataaaattt cgtgaatcat ttcgcccgtt
     1981 tgcaccttct gtcctcgccg agcgggcaca cgaatggttc gatgttccca tcggttacga
     2041 agacccgtac atgctgttcg tcgtcaacgt tcggggcgac gtgacacctt taccggcggt
     2101 cacgcatatc gatggcacgg cgcgggtaca gacggtggat gggcgtcatg gtcggtatcg
     2161 actgctgttg gaagcgtttg cggagcgtac ggggtgtccc gttctggtca acacgagttt
     2221 caacgttcgc ggggaaccga ttgtatgtac gcccgcggac gcatacggtt gttttcttcg
     2281 tacgaacctg gacgttttgg tgttgggccg gtgtgtcgtg gttcgtgatg agcaggtcga
     2341 cgtaccgcgc cacacggtgg ctccgacggg tcaattccca ttggattagg catatggcct
     2401 atacgtgtgg acgaacggga atctggctgt tcgaagcggg gttatccgcg aaagggcggg
     2461 ggtgcggcag gcagggtatc ggcaaagggt agcgtcatca aagctggccg caatcggcga
     2521 tcacgacagg tttgctggtg gcgccattgc gtgagcccac ggcttccatt tgctgaacga
     2581 cttgcatgcc gtcgatcacg cggccgaaca cgacgtgttt tccatcgagc cagctcgtat
     2641 ccgcggtgca aatgaaaaac tgggatccgt tggtattggg cccggcgttg gccatggaca
     2701 gggttccagg cccggagtgc ttgagcttga agttttcgtc ggcgaatttc tcaccgtaaa
     2761 tggattctcc gccggttccg tttccgcgag tgaagtcccc accctggcac atgaagttgg
     2821 ggatgatgcg atggaagctg gagcctttga agtgaagggg ttttccgctt cgacccatgc
     2881 ccttttctcc tgtgcaaagg gcgcggaagt tttcggcggt tttggggacg acgtcggcaa
     2941 atagttcgaa ggtgacgcga ccggcggggg ctcctccgat ggtgatatcg aaaaatacct
     3001 gtggatttgc catgttatgg ttctcctgga tggtgttcgg gcatcaaggt tggggattgc
     3061 gcggctttcg cagttgcatg agccaatagg ccgatgtgcc aagcacggcc acgaccacga
     3121 gcgcgatgat catccatggc gccccttccc atttgcccct tgcagggcct tctggcggtc
     3181 gttccaccac aatggtcggc tgcaacaaca tgcgagcgtg ctgtacactt acagcgggca
     3241 gcatgcaaga agcgccggtg gttacccaat cgatggcaag ggtgatccgt tcatctccgg
     3301 aaatgagcgg gtggaccgtt aggtattgcg ttcgagtcac aaggtgagct gtcgaatccc
     3361 gtgtcgactt gccgacgtgt tcgcttgtgc tcggacattc actccaccat actgggggcg
     3421 acgatgtgct ttgttcttct attccgctcg tcaacgttgc caatgcgatg aggcttgcct
     3481 tcgtttttgc tccttatcac cacaagcggt tcgaggagaa catctcggtg gtggacgagg
     3541 atttcggttt atttccaccc atcaacgtgg cgtatgcggc tgccatcgcg gagcgagcgg
     3601 ggcatacggt gcgtatcttc gatgccaacg cggatattcg attggggcga gcgtcggaag
     3661 accaagtatt gacgcagttg caggaatttc gtccgaacgc cgtaggtcta tatttctcga
     3721 cctatatgtt tcgcgacacg ctggcatggg cgcgtcgtat caaagcgcgg cttgggtgcg
     3781 ttatcttggc gggcggcgtc aattgctcgg tatatcctcg ggagacgtta gagcacccgg
     3841 aaatcgactt catcgtggtg ggtcatgcgg acaagtcgtt gccggccttg ctcgatgcgc
     3901 tcgatggcgg tggcacgatc gattcgattg ctggggtggg gtgcaaggtc gagggggagt
     3961 tggtcgtcc
//
LOCUS       DAOSOR010000544         3966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_22904_length_3966_cov_3.496037, whole genome shotgun sequence.
ACCESSION   DAOSOR010000544 DAOSOR010000000
VERSION     DAOSOR010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22904_length_3966_cov_3.496037"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1509
                     /locus_tag="PK710_21335"
     CDS             <1..1509
                     /locus_tag="PK710_21335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12336.1"
                     /translation="NRADGGAGTTGESGSGGGKSGSGGSSAKGGSGGEDWLDEICFMH
                     DCESDDECVGCPNGRVVCDESSKRCVKCVPGEPPDACDDGEVCSQYGTCIPEGMTCPT
                     DATGEPTVTCTTDNDCFACDTMHQVCDTASGKCVACSDTNLFACDSQASCKQGKCMDK
                     CPYPCTMDEDCASCGTMVNTGHACNETSRHCSECSASHPCPAGSGTVCGPQGMCIKVC
                     GALDKPKGTCQSDDDCKGCEGDLIACHKPINGGDGRCGVPASGCSDIPGNFAVLPEPW
                     SQVTNLCSKDGDCSGVSLDLNVGKILRDLTGLDQIKDAVLPYGMNVCAAASVSVMGKD
                     YACGVCVPCRKDDDCKPIKFDPLIEQAFGPVGSLVAKVLIKMAFGDAPHELNMYCEPV
                     LGEYGVCAPCIDFLHECGNTNTVGSGCHSDWECAAGERCKDGRCQVYKRKDCFGADDC
                     PNGQVCSWNGEGYCCRDIFQGTKTCFSDAECVPQVCAWNGTAFYCTDPVACE"
     gene            1649..2905
                     /locus_tag="PK710_21340"
     CDS             1649..2905
                     /locus_tag="PK710_21340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12337.1"
                     /translation="MQKTPTIVTDDPQIEIEEMGLLDTLQTLDDIELIPEDEDPPKEN
                     TCSNDDAMWVMSSELIQPLGQEDILDARQEDDEPTRFDLRFHPRANQPLFLPNSDPSL
                     PRLEVVDDDRLGMREVIPAGVILPKVVPPPKKQATDSPRMSSIAPVTLATEFDEDNSQ
                     PRLPVVRPAKPRTNLYSATVGALAVAAAVVGFWTLPEIMAATNPPQTVNAVDSPAIVS
                     AHDFENEIQPASIESVVHELEPVIIVGDGMPSASQSASVLEEGQSPKRWEPKQDVLID
                     DSLQLTEGVVFPSHFSDSNAKDEPVSPSTSEPQNEPEPSQPPASSQPSETPSEPAFDT
                     SAASAAMLSAAQAARACAPAGVGRFPARVSVTFAPQTGGVTTATVDSPWIAGTDTTRC
                     IVAKFRSIRVSPFSGPLVTVHKTFEF"
     gene            3030..>3966
                     /locus_tag="PK710_21345"
     CDS             3030..>3966
                     /locus_tag="PK710_21345"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT12338.1"
                     /translation="MRTNRNTNHLVVGAGLAGLSCARELSRAGDTVRILEKDRAVGGR
                     CATHWEQGQPMDYGPVLLHGRACGFEEEIRSIQGITLLEDWPVRRRGAGKPCQHEAYS
                     MTEKRFAIAQGVAAFPEHLARGLDIVLDTRVSEISWTGSTGFHVQSERGEQWNTTSLV
                     LTLPVEPTLAFLWPLSKHSQDIEAIATLLDTLGTLPCLTLMAGYEAAVPDPGWDVLYP
                     ETSDGLMLVSHDSSKRREPAWRVLVFQATPAWSRQHLAMAPEQWAKQLLAEASKVVGE
                     WAAHPQWIKPHAWRHARVDLGNELSGPVRITMPGGA"
BASE COUNT          837 a         1033 c         1231 g          865 t
ORIGIN      
        1 aaccgcgcgg acggcggagc tggcaccaca ggggaatcag gaagcggagg gggaaaatcg
       61 ggttccggag gttcttccgc gaaaggggga agtggcggag aggattggct cgacgagatc
      121 tgtttcatgc atgattgtga atccgacgat gaatgcgtgg gttgcccgaa tggccgggtt
      181 gtctgtgatg agtcttcgaa acgctgtgtg aagtgcgtgc caggggaacc gccggatgcg
      241 tgcgatgacg gcgaggtatg ttctcaatac ggcacgtgta ttccggaagg gatgacctgt
      301 cccacggatg cgacgggcga gcccacggtg acatgcacga cggacaacga ctgttttgct
      361 tgtgatacga tgcatcaggt atgcgatacg gcatcgggga aatgtgtagc ctgcagtgac
      421 accaacctat ttgcctgtga ttcccaagcc tcctgcaaac agggcaagtg catggataaa
      481 tgcccttatc cctgcacgat ggacgaggat tgcgcatcgt gtggaacgat ggtgaacacg
      541 gggcatgctt gcaatgaaac atcgcggcat tgcagtgaat gctcggcctc ccatccttgt
      601 cccgcgggaa gtggcaccgt gtgtgggccg cagggcatgt gcatcaaagt atgcggggct
      661 ttggacaaac ccaaaggaac ctgtcaatcc gatgatgatt gcaaaggttg tgaaggcgac
      721 ctcatcgcct gccacaaacc tatcaatggg ggtgacggtc gttgtggcgt gcccgccagt
      781 ggatgttcgg atatcccggg caacttcgcc gtattgcccg aaccttggtc gcaggtgacc
      841 aacctctgct cgaaagatgg cgattgctcc ggggtcagtc tcgatttgaa cgtgggcaag
      901 atccttcggg atttgacggg attggaccag atcaaagacg ccgtgctgcc gtacgggatg
      961 aacgtatgtg ccgcggccag cgtttcggtc atgggaaagg attacgcttg tggcgtatgc
     1021 gttccttgtc gaaaagacga tgactgcaaa ccgatcaaat tcgacccgtt gattgaacaa
     1081 gcctttggac cggtgggcag ccttgtcgcg aaggtgctca tcaaaatggc ctttggtgat
     1141 gcgccccacg agttgaatat gtattgcgag cccgtgcttg gtgagtatgg cgtgtgtgct
     1201 ccctgcatcg acttccttca cgagtgcggc aacacgaata cggtgggttc cggctgtcac
     1261 agcgattggg aatgcgctgc gggcgaacgt tgcaaagatg gccgatgcca ggtctacaaa
     1321 cgcaaggatt gttttggagc cgatgattgt cccaacggcc aggtatgttc gtggaatggg
     1381 gagggatatt gttgtcggga tatcttccag ggcacaaaga cttgtttttc cgacgcggaa
     1441 tgcgtgcccc aggtctgcgc ctggaatggc accgcatttt attgcacaga tcctgtcgcc
     1501 tgcgaataac caagcctttt ttcccctttc ccgccccttc gctatcaccc ccttgacgaa
     1561 ccctgacttc ggtgccatcc atgtagcaca cggggtgaca ttgggtaagc ttgtacccat
     1621 tacacctcta agacctcgga ggtcggccat gcagaaaaca ccaaccatcg tcaccgatga
     1681 tcctcagatt gaaatagaag aaatggggct attggacacg cttcagacgc tcgatgacat
     1741 cgagttgatc cctgaagacg aagatccgcc caaagaaaat acttgttcga acgacgatgc
     1801 gatgtgggtc atgtcttccg agttgatcca accccttggg caggaagaca tcctggacgc
     1861 gcggcaagag gatgacgagc ccacgcgatt cgatctccgt tttcatcctc gtgccaatca
     1921 accgttgttt ttgcccaaca gtgacccatc gctcccgcgt ctcgaagtgg tggatgacga
     1981 tcgtttgggc atgcgtgagg taattccggc aggtgtcatc ctgccgaagg tggtgccgcc
     2041 tccgaaaaaa caagcaacgg attcaccacg gatgtcttcg atcgctccgg taaccctcgc
     2101 gacggaattc gacgaagaca attcgcaacc tcgtcttccg gtcgttcggc cagcaaaacc
     2161 acgaacgaac ctgtattcgg ccacggtggg cgcgcttgcg gtggctgccg ctgtcgttgg
     2221 gttctggacc ttgccggaaa tcatggccgc aacgaaccct ccccaaaccg tcaatgccgt
     2281 ggattcgccc gcgatcgtct cggcccatga tttcgaaaac gagatacaac ccgcgtccat
     2341 cgagtcggtg gtccatgaac tcgagccggt gatcatcgtt ggtgatggca tgccatccgc
     2401 gagccaatca gcttcggtat tggaagaagg tcaatctccg aagcgttggg aaccgaagca
     2461 agacgtgctg atcgacgact ccctccaatt gacagaaggt gttgtttttc caagtcattt
     2521 ttcagattcg aacgcgaagg acgaaccggt ttctccttct acttccgagc cgcaaaacga
     2581 gcctgaacct tctcaacccc ctgcatcgtc tcagccatcg gagacaccgt ctgagcccgc
     2641 gttcgacaca tcggcagcct cggcggcgat gttgtccgcc gcgcaggcgg cgcgggcatg
     2701 tgcgccagca ggcgtcggtc ggttcccggc gagagtatcg gttacctttg cgccgcagac
     2761 tggcggggtg acgacggcga cggtggatag tccttggatt gcgggaacgg ataccacgcg
     2821 atgcatcgta gcgaagttcc gttcgattcg agtgagtccg ttttcgggtc cgctcgtcac
     2881 ggtccacaag acgttcgaat tctagtattt ccgcgacaac acctatcgct ggagcatcct
     2941 aggattgctc cttgccacct tcgcgaataa aagggcgtaa ggcaggtcgt ggtgatggtt
     3001 tcagggtcaa agacgagatg ggcaacggca tgagaaccaa caggaacacc aaccatctgg
     3061 tggtgggagc tggactggcg gggctttcgt gtgctcgaga gctttcacga gccggcgata
     3121 cggttcgtat cctcgaaaag gatcgagcgg tgggaggtcg ttgcgccacg cattgggagc
     3181 aagggcagcc catggattac gggcccgttc ttcttcacgg tcgggcctgt ggatttgagg
     3241 aagagatccg gtccatccaa ggaatcacgc tgctggaaga ctggccggta cgtcgacgag
     3301 gtgctggcaa gccttgccag catgaagctt attcgatgac ggaaaagagg tttgcgattg
     3361 cgcagggggt ggccgcattt cccgagcact tggcccgggg tctcgatatc gttctggaca
     3421 cgcgagtgtc tgagatttcc tggacggggt cgacgggctt tcatgtacaa tcggagcggg
     3481 gggagcagtg gaatacgacg agtttggtgc tcacgcttcc ggtggagcca acgcttgcgt
     3541 ttctttggcc tttgagcaag cactcccagg acatcgaagc gatcgcgaca ttgctcgaca
     3601 cattgggaac gctgccttgt ttgacgctga tggcggggta tgaggcggcg gttccggatc
     3661 cggggtggga tgtgctgtat ccggaaacat cggatggatt gatgctggta tcccatgact
     3721 cttcgaagcg aagggagccg gcttggcgcg tattggtttt tcaggcgacg cccgcgtggt
     3781 cgcggcagca tctagcgatg gctccggagc aatgggcaaa acagttgctg gcggaagctt
     3841 cgaaggtggt gggcgaatgg gcggcgcatc cccaatggat caagccgcat gcatggcgtc
     3901 atgcgcgcgt ggatttgggc aatgagttgt cgggtccggt acgcatcacg atgcctggag
     3961 gagcct
//
LOCUS       DAOSOR010000545         3955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_22987_length_3955_cov_3.918462, whole genome shotgun sequence.
ACCESSION   DAOSOR010000545 DAOSOR010000000
VERSION     DAOSOR010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22987_length_3955_cov_3.918462"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="PK710_21350"
     CDS             <1..856
                     /locus_tag="PK710_21350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOT12339.1"
                     /translation="GAGAYICGEETALLESLEGSRGEPRLRPPFPAQRGAFGCPTTVN
                     NLESIASCPTILEMGGEQFSKLSSLHKFGDGGCRLYGVSGHVNKPGVYEACIGVTLRE
                     LIYDLGGGMPEGTNLLGVIPGGSSCPIIRPDDIVDAPDPKSILHEWHGKPQLDVPIGV
                     DTMRNLGTMAGTTCAIVLSDRACPVLAMHNLARFYRHESCGQCTPCREGTGWMLRILD
                     KIVQGKGSIEELDMLHEIASNIMGNTICAFGEGASMPMLGFLRKFRKEIEDYIRTGGS
                     SSTGRLAL"
     gene            853..1407
                     /locus_tag="PK710_21355"
     CDS             853..1407
                     /locus_tag="PK710_21355"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOT12340.1"
                     /translation="MINAIVEQGLFYVSALLAVAGALGTVASKSAIRSAMSLLATIVG
                     IAGLYLTLSAQLLAAIQILVYAGAIVVLFVFVIMLLGGQVQPNQDKRTSSSRAIGAGL
                     FALSAILAAVLVARAGLGGPHLFDPPRIEFGSVSELGRVMFTDGVIAFELIGVLLTTA
                     IVGVMAVARTRHAKTNAKSKEVGP"
     gene            1404..1715
                     /gene="nuoK"
                     /locus_tag="PK710_21360"
     CDS             1404..1715
                     /gene="nuoK"
                     /locus_tag="PK710_21360"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOT12341.1"
                     /translation="MTSIPLEYYLVVAAVIFVIGACGFLARRNALIVLMSIELMLNAV
                     NLTLVAFNRWYASHHVGQMFTFFIIAVAAAEAAVGLAIVVALFRLKRTVRTDSVDLLK
                     N"
     gene            1733..>3955
                     /gene="nuoL"
                     /locus_tag="PK710_21365"
     CDS             1733..>3955
                     /gene="nuoL"
                     /locus_tag="PK710_21365"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOT12342.1"
                     /translation="MESLLSLFPNPEDNFPLIAVILGLPLMGALINGIFGKRLGPEGV
                     RTMALSAVGGSFLASLAAFLLLYARVQQASASLSEGAAVPHVKFVWNAWRWLALETTS
                     IDVVFAIDSLSATMMLVVTGVGGLIHLYSTGYMAGDPSYHRFFAYLNLFIFSMLVLIL
                     GDNLPILFVGWEGVGLCSYLLIGFWFEADKNASAGKKAFITNRIGDFGLLFAMAMILH
                     TTGSLSWAGIEARAPELFNELQIWPIGVAPSWLPAFLTSPVVVNYATLIGLALFLGCA
                     GKSAQIPLYVWLPDAMAGPTPVSALIHAATMVTAGVYLVARMSFVFVLSPTAMMVVAG
                     VGAFTALFAATIGLVQHDIKKVLAYSTVSQLGFMFMGVGVGAFASGMFHVVTHAFFKA
                     CLFLGAGSVIHAMHARIHDTDASQDMRNMGGLRKYMPKTYWTYMAATAAIIGLPLTAG
                     FFSKDDILFRAFVNRIQPSGLLKDLAIRNAVQKAGAPLSDEAREAVVQGLHIPEWFST
                     AVFVVGVTAAILTAFYMLRTVFLTFFGDFRGWRIVPGWKNDDHDHDHHASHAHDEPID
                     GPVPHESPWAMWVPLVVLAFFALFAGWVNAGPFHLTAFSHWLEPSMQSGLKMVTTIKQ
                     GDELTHLEHLLLIPGVLAFLIGSGAAYWVYYVKKGEPARQAAQSAGGLYRLVLDKWRI
                     DELYEATVLGAFHSLAETAALFDKWVIDGVLARLTTMVIQSFGSLFGAFQTGRVQRDA
                     A"
BASE COUNT          677 a         1155 c         1162 g          961 t
ORIGIN      
        1 gggtgccggc gcgtatatct gcggcgaaga aaccgcgctt ctcgaatcgc tggaaggaag
       61 ccgtggcgag ccgcgtcttc gtcctccctt ccctgcccaa cggggcgctt ttgggtgtcc
      121 tacgacggtc aacaatctcg aatccattgc ctcttgtccg acgatcctcg agatgggggg
      181 agagcagttc tcgaaactat cttcgttgca caagttcggt gatggtggat gcaggctgta
      241 cggggtgagc ggtcacgtca acaagcccgg tgtctacgaa gcttgtatcg gcgtgacgct
      301 gcgcgaattg atttacgact tgggcggggg catgccggaa ggaacgaacc ttttgggtgt
      361 gattccaggc ggttcttctt gccccatcat tcgtcccgac gacatcgtcg atgcgccgga
      421 ccccaaatcg attctgcacg aatggcatgg aaagccgcag ctcgacgtgc ccatcggggt
      481 cgatacgatg cgcaatctgg gcacgatggc gggtaccacg tgtgcgatcg tattgtccga
      541 tcgcgcctgt ccggtgctcg ccatgcacaa tctcgcgcgg ttttatcggc atgagtcttg
      601 tggtcaatgc acgccttgtc gggaaggaac aggttggatg ctgcgcatcc tcgacaagat
      661 cgtgcaggga aaaggctcga tcgaagaact cgatatgctt cacgaaatcg catccaacat
      721 catgggcaac accatctgcg cctttggcga aggcgcctcc atgcccatgc ttggtttcct
      781 tcggaaattc cgaaaagaaa tcgaagacta cattcgaacg ggtggttcgt cttccaccgg
      841 gaggctcgcc ctatgatcaa cgcgatcgtc gaacaaggat tgttctacgt ttccgcgctg
      901 cttgccgtcg cgggagcgtt gggaaccgtc gcttcgaaaa gcgccattcg ttccgccatg
      961 tcgctgctgg cgaccatcgt cggcatcgcc gggttgtact tgacgctttc cgcgcagctt
     1021 ctcgcggcga ttcagatttt ggtctacgcc ggcgcgatcg tggttctgtt cgtattcgtc
     1081 atcatgctgc tgggcgggca agtgcagccg aatcaggaca agcgaacctc gtcgagtcgc
     1141 gcgattggag cggggctttt cgccctcagc gcgattctcg cggcggtgct cgtcgcgcgc
     1201 gcgggccttg ggggacccca tctcttcgac ccgcctcgga tcgaattcgg ctccgtcagt
     1261 gaacttggaa gggtcatgtt caccgacggc gtcatcgctt tcgaactcat cggtgtgctg
     1321 ctgaccacgg ctatcgttgg cgtgatggcg gtcgcgcgta cgcgtcacgc caagaccaac
     1381 gccaagtcca aagaggtggg cccgtgacct ccattcctct cgagtattat ctcgtcgttg
     1441 cggcggtgat atttgtcatc ggcgcgtgtg gttttctcgc gcgacgcaac gctctcatcg
     1501 ttttgatgag cattgaattg atgctcaacg cggtgaacct gaccctggtg gcattcaatc
     1561 gttggtatgc gagccatcat gtgggtcaga tgttcacctt cttcatcatt gcggtggcgg
     1621 ctgcggaagc cgccgtgggt ctcgccatcg tggtcgcgct gtttcgtctc aagcgcacgg
     1681 tgcgaaccga ttcggtcgac ctcctcaaga actgagcgcc ctggagaccc tgatggagtc
     1741 attgctctcg ttgttcccaa accctgaaga caacttcccg ctcatcgcgg tcatcctcgg
     1801 tttgcccttg atgggtgcct tgatcaacgg catcttcggc aaaaggctgg gccccgaggg
     1861 ggtgcgcacg atggccttat cggcggttgg cggctctttc ctcgcctcgc tagccgcttt
     1921 ccttctgctg tatgcccgcg tgcaacaggc ctctgcctcc ctttccgaag gcgcggccgt
     1981 ccctcacgtc aaattcgtct ggaatgcatg gcgatggctc gctctcgaaa ccaccagcat
     2041 cgatgtggtt tttgccatcg atagcctgag cgccaccatg atgcttgtcg tcacgggcgt
     2101 gggtggcctc atccatctgt attccacggg gtacatggcg ggagacccga gttatcaccg
     2161 ctttttcgcc tatctcaacc tgttcatctt ctcgatgttg gtgctgattc tgggcgataa
     2221 cctgccgatt ctcttcgtgg gatgggaagg cgtggggctg tgcagctacc ttctcatcgg
     2281 gttttggttc gaggccgaca agaacgcttc ggcgggtaaa aaagcgttca ttaccaatcg
     2341 catcggtgac ttcgggctgt tgttcgccat ggcgatgatt ctgcacacga ccggcagtct
     2401 gtcttgggcg gggatcgagg ccagggcacc ggagctattc aacgaactac aaatctggcc
     2461 cattggtgtg gctccttcct ggttgcctgc ctttctcacg agccctgtcg tcgtcaacta
     2521 cgccacgctc atcggccttg cgctatttct tggttgcgcg ggcaagagtg cgcaaatccc
     2581 tctgtatgta tggcttcccg acgccatggc ggggcccacg cccgtgtctg ccctcatcca
     2641 cgcggccacg atggttaccg ccggtgtgta tctcgtggct cgcatgagtt tcgtattcgt
     2701 gctttcgccc actgcgatga tggtggtcgc tggggttggt gcgtttacgg ctctgtttgc
     2761 cgccacgatc gggctcgtgc agcatgacat caaaaaggtg ttggcttatt ccaccgtcag
     2821 ccaactgggg ttcatgttca tgggcgtggg ggtgggcgcc ttcgcctcgg gcatgttcca
     2881 tgtcgtcacc cacgcctttt tcaaagcttg tttgttcctc ggggccggtt cggtcattca
     2941 cgcgatgcac gcgcgcatcc acgataccga tgcctctcag gacatgcgca acatgggtgg
     3001 tttgcgcaaa tacatgccca aaacatactg gacatatatg gcagcgaccg cggcgatcat
     3061 aggcttgccg cttaccgctg gcttcttttc gaaagacgac atcctgtttc gagccttcgt
     3121 caaccgcatc cagccttcgg gattgctcaa agacctggcg attcgaaacg cggtgcaaaa
     3181 agctggcgct ccgctatccg acgaagcgcg cgaagcggtg gtgcagggcc tgcacatccc
     3241 cgaatggttc tccaccgccg tattcgtcgt gggtgtcacc gccgccatcc tgaccgcgtt
     3301 ttatatgctt cgcaccgttt tccttacgtt tttcggggac ttccgtggat ggcgcatcgt
     3361 gcccggctgg aaaaatgacg accacgatca tgaccatcat gcgtcgcacg cgcatgacga
     3421 acccatcgac ggtccggtgc cgcatgagtc tccatgggcc atgtgggttc ctctggtggt
     3481 gcttgctttc ttcgctctct tcgcaggctg ggtcaacgcg gggccgttcc atctcaccgc
     3541 cttttctcat tggctcgagc cttccatgca aagtggactc aagatggtga cgacgatcaa
     3601 acagggagat gaactcacgc acttggagca tctgctgctc atccccggtg tgctggcctt
     3661 cctgatcggc tccggcgcgg cctattgggt gtattacgtc aaaaaaggcg agcctgcccg
     3721 gcaagccgcg caatccgccg gaggcttgta ccgtctggtg ctcgataaat ggcgcatcga
     3781 cgaattgtac gaagccaccg tgcttggtgc tttccattcg ttggcggaga ccgcggcgtt
     3841 gtttgacaaa tgggtcatcg acggcgtgtt ggcgcgattg accacgatgg tgattcagtc
     3901 cttcggttcg cttttcggag ccttccagac gggccgtgtg cagagggatg cagcg
//
LOCUS       DAOSOR010000546         3944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_23063_length_3944_cov_3.818205, whole genome shotgun sequence.
ACCESSION   DAOSOR010000546 DAOSOR010000000
VERSION     DAOSOR010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23063_length_3944_cov_3.818205"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PK710_21370"
     CDS             complement(<1..481)
                     /locus_tag="PK710_21370"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOT12343.1"
                     /translation="MTLPTAGFSFHVQAREGAARAGVLDTPHATVLTPTFMPVGTQGS
                     VKGLSPEEVAATGARIVLGNTYHLWIRPGPEEIDALGGLHSFCRWPHAMLTDSGGFQA
                     FSLTNLVKLSEDGFAFRSHLDGSRMHLSPEEAMRVQGLIGADIAMQLDVCPAANADST
                     "
     gene            complement(478..1575)
                     /gene="queA"
                     /locus_tag="PK710_21375"
     CDS             complement(478..1575)
                     /gene="queA"
                     /locus_tag="PK710_21375"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOT12344.1"
                     /translation="MRIDAFDFDLPAERIAQHPVDPRDASKLLVLDPPTDSLFDRHVF
                     DLPDLLPTGALLIVNDTRVIRARLIGRKAETGGKVELLLVRRLGDESDGGRVVAEQWR
                     AMGKASKAFRFPATILFGDPPCIVAVLRSRAPDGLLDVSLASTNGSSVAQALERVGHI
                     PLPPYIRREDESSDAERYQTVFARHDGAVAAPTAGLHLTHRLLGALSARGFEIATITL
                     HVGPGTFQPVMVDDLDGHTMHEEWFDISHHTVKAIEAARQRKAPVIAVGTTCVRALES
                     AADPEREGMVRPGQGTTRLLIQPGYRFRVVDGMLTNFHLPRSTLLALVSAMAGRERIL
                     RAYQHAIDREYRFFSYGDAMLILRAEGTRWM"
     gene            complement(1625..1990)
                     /locus_tag="PK710_21380"
     CDS             complement(1625..1990)
                     /locus_tag="PK710_21380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT12345.1"
                     /translation="MASARILIVDDQESMRKTLSIALRLDGFDVACAASATDALESLS
                     CRPCDLAIVDLMMPDVNGLDLARQLRTLYPNLKVVLTSAYHLSERQVLRSQCGAIGFV
                     PKPFQMSELIAFLRDKALS"
     gene            complement(2159..3508)
                     /locus_tag="PK710_21385"
     CDS             complement(2159..3508)
                     /locus_tag="PK710_21385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOT12346.1"
                     /translation="MAKILVVDDQPNMRTTLVMMLRGAGYDVDAAVDGTKGSELGATG
                     AYDVVLTDLRMGAVGGIEVLRNIKQKMPMTEVVVMTAYGTIESAVEAMRLGAFDYIQK
                     PFAEQELLMKVQRALDNRRLAGQVQLFANEFKERYHFENIIGRSQSIRDVLARIVKIA
                     PSDATVLITGESGTGKELVAKAIHANSRRNTKPFVPVNCAAITETLLESELFGHARGA
                     FTGAVGARKGLMEEADGGTFFFDEIAETPMSFQAKLLRAIQENEIRRVGENKPIKVDV
                     RIIAATNQDLLRAIEDKTFRQDLYYRLNVARFELPPLRERREDIPLLADFFLQKYNRK
                     NGVKARLGEGVVEALMQHDFPGNIRELENLLEQGVALSGGGIVGLDEVEMRVTRSSST
                     SGRTLAEVVDSAEKQAIEDALRECDGSRERAAELLAISPTTLWRKMTRLGVVYDSRS"
     gene            complement(3568..>3944)
                     /locus_tag="PK710_21390"
     CDS             complement(3568..>3944)
                     /locus_tag="PK710_21390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT12347.1"
                     /translation="REVVAKMLEKQQRPGVIVNISSIARHGNRGQSNYVSAKAAIAQN
                     TKTWSQEFAPFGIRVRAVAPGMVETPMTQGMNAKAREALVNMIPVGRIGEPEDIWQAV
                     RFVIECDYFNGRCIDVDGGLSM"
BASE COUNT          888 a         1168 c         1061 g          827 t
ORIGIN      
        1 cggttgagtc ggcatttgcg gcagggcaga catccagttg catcgcgata tccgccccga
       61 tgaggccttg aacccgcatc gcctcctcgg gagacaaatg cattcgagag ccgtcgaggt
      121 gggagcgaaa cgcaaaaccg tcttccgaca gtttcacgag attcgtgagt gaaaacgctt
      181 gaaatccacc agaatcggtg agcatcgcat ggggccagcg gcaaaaggaa tgcaatcccc
      241 cgagggcatc gatttcctcc ggacccgggc gaatccataa gtgataggtg ttgccaagca
      301 cgatcctggc gccggtggcc gcgacttctt caggggacaa ccccttgacg gacccttgcg
      361 ttccgacggg catgaacgtt ggtgtcaaca cggttgcatg cggtgtatca agcacgcccg
      421 cgcgggctgc tccctcacga gcctgcacgt gaaacgaaaa accggcggtg ggaagagtca
      481 catccacctc gtgccttctg cacgcagtat gagcatcgca tcaccatagc tgaaaaaccg
      541 gtattcgcga tcaatggcgt gttggtacgc cctcaaaatg cgctcgcggc ccgccattgc
      601 gctcaccaac gcaagcaacg tcgaccgcgg caaatgaaag ttcgtcaaca tgccgtccac
      661 cacccgaaac cggtacccag gctgaatgag cagccgcgtg gttccttgtc ctggacgcac
      721 catgccttca cgttcggggt ccgcagcgga ttccaaagcc cgtacacacg tcgttcccac
      781 cgcaatcacc ggtgcttttc gctgtcgcgc cgcctcgatg gccttgaccg tatgatgcga
      841 aatatcaaac cactcctcat gcatcgtatg cccgtccaag tcatcgacca tcacaggctg
      901 aaacgtaccg ggccccacgt gcaacgtaat cgtcgcgatt tcgaatcctc gggcgctcaa
      961 cgctccaagc agccgatgcg tcaagtgcag ccctgccgta ggagccgcca ccgcaccgtc
     1021 atgtcttgca aatacagttt gatatcgctc ggcatccgat gactcatctt ctcttcggat
     1081 atacggcggc aggggaatat gacctactcg ttcgagggcc tgggcaacgc tcgaaccgtt
     1141 cgtactggct aacgacacat cgagcaaacc gtccggcgcc cgagaccgca acaccgccac
     1201 gatgcacggc ggatcaccaa atagaatcgt cgcgggaaaa cgaaatgcct tcgacgcctt
     1261 acccatggcg cgccactgct cggccaccac ccgcccaccg tcgctctcat ctcccaaacg
     1321 tcgaacgagc agcaactcca ctttgccgcc cgtttccgct tttctgccaa tgagacgcgc
     1381 acggatcacg cgcgtatcgt tgacgatgag caaagcaccg gtgggaagca gatctggcaa
     1441 gtcaaagaca tgccggtcaa acaggctatc ggtgggagga tccaacacca gcagtttcga
     1501 agcatcccga gggtccacgg ggtgctgagc gatacgctcc gcgggaaggt cgaaatcaaa
     1561 agcatcgata cgcacgatgg cggtcgccta gcacagcttt taccaagggt ggaggttcag
     1621 gacgtcaaga aagggctttg tctcgaagaa aagcgatgag ttcggacatc tgaaacggct
     1681 tgggaacaaa gccaattgca ccgcattggg agcgaagcac ctgacgctca ctgaggtgat
     1741 aggcactggt gaggacgact ttcaaatttg gatagagggt ccgcagttgc cgagccaaat
     1801 ccagcccgtt gacgtcaggc atcatcaagt ccacgatggc caagtcgcac ggacgacacg
     1861 aaagcgattc gagagcatcc gtagcggaag ccgcacacgc cacatcgaat ccatcgagac
     1921 gcaatgcgat ggacaaggtc tttcgcattg attcttggtc gtccacgatg agtatgcgtg
     1981 cggaggccat acgacgaggt gggaatgcaa accctgcacc agtgcctacg gtccagagtt
     2041 attgtgtaaa taccatgagt taggcatcaa agggatagag aatgccagga tgtcgttgca
     2101 aagacgaagg ggaagagtcg cagggctgca atggcaatga ggccagggct cgaattggtt
     2161 acgatcggga atcgtaaacg acaccgaggc gcgtcatttt tcgccacaag gtggtcggag
     2221 agatggcgag cagttcagcc gctctttccc ggctcccatc acattcgcgc agcgcatctt
     2281 caatcgcttg cttttctgcc gaatcgacaa cctccgccag cgttctaccg cttgtgctgg
     2341 aagaacgtgt cacccgcatt tctacctcgt cgagtccgac gatgccacct cctgacaagg
     2401 ctaccccctg ctccagcaga ttctcgagtt cacggatatt gcctgggaag tcatgctgca
     2461 tgagagcttc cacgacgccc tctcccagtc gagctttgac accgtttttt cggttgtatt
     2521 tttgcaaaaa gaagtcagcg agaaggggaa tgtcttcgcg acgttctcgc aggggcggaa
     2581 gttcgaatcg agcgacattg agcctataat aaagatcctg tcgaaacgtc ttgtcctcga
     2641 tggctcgcag caagtcttgg ttggtagccg cgatgatgcg cacatcgact ttgatgggtt
     2701 tgttttcccc cacgcgacga atctcgtttt cctggatggc acgcaataac ttggcttgaa
     2761 acgacatcgg cgtttccgcg atttcgtcga agaaaaacgt gccgccgtcg gcttcttcca
     2821 tgagtccttt gcgggctccc acggcgcctg tgaacgcgcc cctcgcatgg ccgaaaagct
     2881 cgctttccaa aagggtttcg gtaattgccg cgcaattcac ggggacgaaa ggtttggtat
     2941 tccgacgact gttggcgtga atcgctttgg ccacgagttc tttgcccgtt ccactctcac
     3001 cggtaatcaa cacggtggca tccgacgggg cgattttcac gatccttgcg agaacatcgc
     3061 ggatcgattg cgaacgtcca atgatgtttt cgaaatgata acgttccttg aactcgttgg
     3121 cgaagagctg gacttgtcca gcaaggcgac ggttatcgag ggcgcgctgc accttcatga
     3181 gcagctcttg ctcggcaaaa ggtttctgaa tgtagtcgaa tgccccgaga cgcatggctt
     3241 ccaccgcgct ttcgatggtg ccgtaagcgg tcatgaccac cacctcggtc atgggcattt
     3301 tctgtttgat gttgcgcaac acctcgatgc caccgaccgc ccccatccgc aggtcggtga
     3361 gtacgacgtc gtaagcgccc gtggcgccaa gttccgagcc tttggtgcca tcgacagcag
     3421 cgtccacatc atacccagcg ccccgaagca tcatgaccaa ggtggtccgc atattgggtt
     3481 ggtcatccac taccagaatt tttgccatct gccgtcctgt acctcaaaac atcatcgtcg
     3541 tcgtcaccca ctgccgtcga gctgtcatca catcgacaag ccaccatcca catcgatgca
     3601 tcgaccattg aagtaatcgc attcgatgac aaaacgaaca gcttgccaaa tatcctcggg
     3661 ctcgccgatt cgtcctacgg gaatcatgtt cacgagcgcc tccctcgcct tggcgttcat
     3721 gccttgtgtc atcggtgtct ccaccatacc cggagcgacc gctcgcacgc ggataccaaa
     3781 gggagcaaac tcttgggacc aggttttggt gttctgagcg atcgcggctt tggccgatac
     3841 atagttggat tgcccgcggt ttccatgacg tgcgatcgaa ctgatgttga caatgacccc
     3901 tggacgctgc tgtttttcga gcatttttgc cacgacctcg cgca
//
LOCUS       DAOSOR010000547         3935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_23125_length_3935_cov_4.568814, whole genome shotgun sequence.
ACCESSION   DAOSOR010000547 DAOSOR010000000
VERSION     DAOSOR010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23125_length_3935_cov_4.568814"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PK710_21395"
     CDS             complement(<1..667)
                     /locus_tag="PK710_21395"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12348.1"
                     /translation="MSDKMIPADRKPEFVTIDGNTAVVHVAHAVNEVIAIYPITPSSV
                     MGELADEKSAAGETNIWGSVPHVVELQSEGGASGAVHGALQVGALTTTFTASQGLLLM
                     IPNMFKIAGELTSTVFHVSARAVASHALSIFGDHSDVMACRSTGWALIASGAIQECMD
                     MAMIAHAATLEARVPFIHFFDGFRSSHEVQKIESLSKDDMRYMIQEDLVHAHRARGLS
                     PDRP"
     gene            complement(734..1723)
                     /locus_tag="PK710_21400"
     CDS             complement(734..1723)
                     /locus_tag="PK710_21400"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOT12349.1"
                     /translation="MTDLSTTYLGLSLRSPLVVSSSSLTDSVEKLKSYEENGAGAVVL
                     KSIFEEQIIKESEDFDATLGRGAQSSPEADSYFANIPMEIGPQEYVRLVESAKKSIHI
                     PVFASINCVSAKSWTGYAKQLENAGADALELNLYYLPTDPLKTSADIEKNYIDIVQQV
                     RSQVKIPIAVKLGPFLTAIPHTVAQIAKAGAQGVVLFNRFYQPNLDLDELKIVARLQL
                     SRPEEVLLPMRWIAILNGRIDIDFAATTGIHDGISALKVILAGASVAQVASAIYKHKA
                     PHLKVMLRDMETWLREKEYASVADIRGILSQKTCEDPEGFERAQYIKVLVGHG"
     gene            complement(2067..3167)
                     /locus_tag="PK710_21405"
     CDS             complement(2067..3167)
                     /locus_tag="PK710_21405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12350.1"
                     /translation="MIDISAQEPWTCVLGYFRRIVGVALFLLCGSCVSLGLMVALGSS
                     HAVAAPTAQDETKARQLFNEGVEALNRGQFTEALDKFEQAYGLWNNPKILLNIATTLR
                     ELDRLIEAAQAYEAYFENPNADRSRIPEVQNVLSEIDKKIVSLTVEVRTEGSTLRVDG
                     APIEHRTSDAPGKWFVRLMPGEHILVISKEGFVSQTEKFSAHAGEKVTFRVRLEPEEV
                     QPTAMEQPELEDTEDDGLFSHAGQFGLVARVDVDGKLRGAVGVVGVTYGLGDWFEGQV
                     AALNGRDKGFEVDVSWLMTRGRFKPVVSIGIPTFFVDSGRVGIHGAVGLQVDPSRHWG
                     VLAQVGGASFFNAPDNRESFAFVPALGVQGRF"
     gene            complement(3348..>3935)
                     /locus_tag="PK710_21410"
     CDS             complement(3348..>3935)
                     /locus_tag="PK710_21410"
                     /inference="COORDINATES: protein motif:HMM:NF014327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HOT12351.1"
                     /translation="GSGVIFGATGGVMEAALRTVIELVCGIKVENLFEHADIIPLRGF
                     EAIRMMELKIPKVGPVPDILKKNKDLPFDSWDWLLGATLKVAVAHGTANAKRVLEDIK
                     AGGPLSECHFIEFMACPGGCLGGGGMPIPTSPEIRAARAKAIYAEDASYEVRKSHENP
                     AVMRVYHEFLTDGPCGHTAHTLLHTHYTARGKYIE"
BASE COUNT          881 a         1199 c         1006 g          849 t
ORIGIN      
        1 ccggtctatc cggtgacaga cctcgagcac gatgggcatg aaccaggtct tcctgaatca
       61 tgtaccgcat gtcgtctttg ctcaacgact cgattttctg cacttcgtgc gaggatcgga
      121 agccatcgaa gaagtgaatg aacggtacgc gcgcttcgag cgtcgccgcg tgagcaatca
      181 tagccatatc catgcactct tgaatggctc cggaggcgat caaagcccat ccggtgctac
      241 gacaagccat cacgtccgaa tggtcgccga agatcgaaag cgcgtgggag gccacggcgc
      301 gagccgagac gtgaaatacc gtcgaggtaa gctcgcccgc gatcttgaac atatttggga
      361 tcatcagcag caaaccttgc gaagcggtaa atgtcgtggt caatgcaccg acttgcaacg
      421 cgccatgcac cgccccggaa gcaccgcctt cggattgtaa ttcgacaaca tggggaaccg
      481 atccccagat gttggtttct cccgccgcgg atttttcatc cgcgagttca cccatcacgg
      541 acgacggggt gatgggatag atcgcaatga cctcgttgac ggcgtgagcc acatgcacca
      601 ccgcggtgtt gccgtcgatg gtcacgaatt caggctttcg gtcggccgga atcatcttgt
      661 cagacatgga acctccaaat agcgttcatc ccttccgaag aagggtctcc atcgttatcg
      721 agaaagggcg cggtcatccg tggccgacca gcaccttgat gtattgggca cgttcgaatc
      781 cttccggatc ctcacaggtt ttttgagaca agatgcctcg gatatccgca acggatgcgt
      841 actccttctc ccgcaaccac gtctccatgt cgcgcaacat caccttgaga tgaggggcct
      901 tgtgcttgta gatcgccgag gcaacctgcg ccacactcgc acccgcgagg atgactttga
      961 gagcggaaat cccatcatgg atgccggtgg tggcagcgaa atcgatgtcg attcgaccgt
     1021 tgaggatggc gatccaccgc atcggcagca ggacttcctc cggacgagaa agctggagcc
     1081 gtgccacgat ttttagttcg tcgagatcca gattgggctg atagaaacgg ttgaagagca
     1141 cgaccccctg cgcgcccgct ttggcgatct gcgcgacggt atgcggaatg gccgtcagga
     1201 acggacctag tttgacggcg atgggaattt tcacttgcga gcgaacttgc tgcacgatgt
     1261 cgatatagtt tttttcgatg tccgcgctgg ttttcagcgg gtcggtgggc agatagtaca
     1321 ggttgagttc gagagcatca gcgcccgcgt tttcgagttg tttcgcatag cccgtccagc
     1381 ttttggcgga cacgcaattg atgctggcga atacggggat atgaatgctt ttcttggcgc
     1441 tttcgaccaa ccgaacatat tcctgtggtc cgatttccat cggaatattc gcgaagtagc
     1501 tatccgcttc cggactgctt tgcgccccac gacccagcgt ggcgtcgaag tcctccgatt
     1561 ccttgatgat ctgctcttcg aagatcgact tgagcactac cgcgcccgcc ccattctcct
     1621 catagctctt gagcttctcc acgctgtcgg tcaacgaaga actcgacacg acaaggggac
     1681 tgcggagtga tagccccaga tacgtcgttg acaaatcggt catactggca tcctttcctt
     1741 ccgtcgatca cgtggaaccc aagggtttgc cacgcttggc atttcattcg acccacggag
     1801 cttcgcacct actggttgcc accgctccgg cagccacacc cacagcatac tcacagggcg
     1861 ggagattcag tcggcgctgt tataccgatt tgcccaaggg attcaatcca actcgcacct
     1921 gctattgctc ttctatgcac cgtagctgac gcaacggaac ccaaatgagc gatttcatga
     1981 gcccccattt cgctcaacgg tggaagcatt cggctcccag caccccgttt cgccatcgga
     2041 agccagcttc gcgcgaaggt tctccctcaa aacctgccct gaactccaag ggctggaaca
     2101 aaagcaaacg actcccgatt gtccggggca ttgaaaaaac tagccccccc cacctgtgcc
     2161 aacacgcccc aatggcgcga tgggtccacc tgaagtccta ccgctccatg aattccgact
     2221 cttccggaat cgacaaaaaa cgttggaatt ccaatagata caaccggttt gaagcgtccc
     2281 ctcgtcatca gccacgacac atccacttca aatcccttgt cgcgaccatt cagagccgcc
     2341 acctgccctt caaaccaatc cccaagccca taagtaaccc ccacaacgcc gacagcacct
     2401 cgcagttttc catccacatc cacgcgcgct accaatccaa actgccccgc gtgactgaac
     2461 aaaccatcat cctccgtatc ctccagctcc ggctgctcca tcgctgtcgg ttgcacttcc
     2521 tccggttcga gtctcacccg aaacgtcacc ttctcccccg catgcgccga aaatttttcc
     2581 gtctgggaga caaatccctc tttcgaaatg accaaaatat gctcccccgg cattagtcga
     2641 acgaaccact ttccaggcgc atccgatgtc cgatgctcga tgggcgcgcc atccacgcgt
     2701 agcgtcgaac cttctgtcct gacctctacc gtgagggaga caattttttt gtcgatttcg
     2761 gagagtacat tctgcacttc cggaatccgg ctccgatcgg cgttgggatt ctcgaaatag
     2821 gcttcataag cttgggccgc ctcgatcaat cgatcgagtt cccgaagagt cgtggcgata
     2881 ttgagaagga tcttcggatt gttccaaagc ccgtacgctt gctcgaactt gtcgagcgct
     2941 tcggtaaact gcccccggtt aagcgcttcg accccttcat tgaacagttg tcgcgccttc
     3001 gtttcgtctt gcgccgtagg cgcggccacc gcatgcgacg agccgagcgc gaccatcagc
     3061 ccaagggaaa cacacgagcc acacagcaga aaaagagcca cacccacgat tcgacggaaa
     3121 taacccaaaa cgcaggtcca cggctcttgg gcagaaatat caatcatgac actccgctcg
     3181 agactaaacc acagagtacg cgcacaaggc aattagacaa tccggtggtt cgacggaagg
     3241 catggggcat cgatttccag cctctgctcg atgcaagatt cgcctccatc gtcgcgtgaa
     3301 cgaaggcgtc gcgtgaacga agggatccgg tgggttcaag aagcaggcta ttcgatgtac
     3361 ttgccgcgcg cggtgtaatg ggtatggagc aatgtgtgtg ccgtatgtcc gcacggacca
     3421 tcggtgagga actcgtgata cacgcgcatc accgcgggat tttcatggga cttacgaacc
     3481 tcgtaggaag catcctctgc gtagatggct ttggcacgag ccgctcgaat ctccggtgag
     3541 gtcgggatcg gcatacctcc gcctccgaga caaccgccag ggcacgccat gaactcgatg
     3601 aagtggcatt cggaaagcgg accaccagct ttgatgtcct cgaggacacg cttggcattg
     3661 gcggtaccgt gcgccacggc caccttcagg gttgccccga ggagccaatc ccaggagtca
     3721 aacggcaagt ccttattttt cttcagaata tccggaactg gtcctacttt cggtatcttc
     3781 aattccatca tgcgaatggc ttcgaagcca cgaaggggaa tgatatccgc gtgctcgaac
     3841 aagttttcca ctttgatacc acacaccaat tcgatcacgg tgcgcagtgc cgcttccatc
     3901 acgccccctg tcgcaccgaa aatgacgcct gaccc
//
LOCUS       DAOSOR010000548         3933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_23143_length_3933_cov_3.546416, whole genome shotgun sequence.
ACCESSION   DAOSOR010000548 DAOSOR010000000
VERSION     DAOSOR010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23143_length_3933_cov_3.546416"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PK710_21415"
     CDS             <1..346
                     /locus_tag="PK710_21415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12352.1"
                     /translation="ILATHALMRELRPNMIQVAFFTPYPGAPVHESVRERHLEPQAFS
                     HYNRLINLSQVSDEALRSWQRKLYWDFLMNRRFIAEYLRFETIPALVNLDQFLPFLRL
                     SAKFLTRLNKSQ"
     gene            complement(403..1722)
                     /locus_tag="PK710_21420"
     CDS             complement(403..1722)
                     /locus_tag="PK710_21420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT12353.1"
                     /translation="MAKWFPYIVASLAMGTMGFMACSSDSSDASSHGATAGDASVGDA
                     KPATGGTGGVGGSGGDGGAGASTGSTGGAGGFEWPEGGSSGSSATGGSGGTGFDAGPD
                     VDFSYDAPPTPDGFNPDATCAETIVEAQLTPLDMYIMMDKSSSMEGARWNNVVAAVKG
                     FVDDSASAGIGVGLQYFPVLPSGYTSTCSSDGQCGNYGPCWNTYCRACATTHYSTPAV
                     GITTLPGGAGAIKSSLNATSPFGGTPTGPALTGAIQYATNWANTHPGHTVVVVLATDG
                     DPYSCTPSTIPEISNVAAAGKNQTPSVLTFVIGVGTSLANMNAIAAAGGTQKAYLVDT
                     GGNVTQQFIQALNDIREKSLGCEYVMPTTDAGVIDPALVQLTFTPGGGTPRNVPQVDD
                     ASQCAGEGWYYNDKNNPTRLILCPSFCSTVQADKNGKIEISLGCLSS"
     gene            complement(1754..3007)
                     /locus_tag="PK710_21425"
     CDS             complement(1754..3007)
                     /locus_tag="PK710_21425"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT12354.1"
                     /translation="MAALRRCVLLVHPSRPRERTYPLGLAYLAAAAKRAGALVLGCDL
                     RIDPPAKLVELLRQHPFDYVGITALSTTLSEAAFIVQQVRRIQPASFVVVGGPHATLA
                     PRQTLQATRADAIVRGDGETPFAALLNGSLDVPGVTLRGQTPDDHVSVEPHLDRLPFA
                     DRSVFPLEAYYRRGVFGSSRRTNLIVTRGCPLSCGYCNANALSRGTFRLRSIEQVMQE
                     VQRLRRDYHVDTLVLEDDNIGLDRDYALRLWRAFSEQNPRFRIDLPNGYHPALVDEEL
                     IDAMAAAGVRSIAFGIESLDENHRILLHRHFDLDRFLPIVAYAQRKNIVTTGYILIGI
                     DGQSARQAIQSASSLRTLPFDMVHVSVLLDLPNQGQVVPSRTWKTVRAIMYVRIHLTP
                     RRILRLMRLAAGSSRTDFDKPNRWC"
     gene            complement(3014..>3933)
                     /locus_tag="PK710_21430"
     CDS             complement(3014..>3933)
                     /locus_tag="PK710_21430"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT12355.1"
                     /translation="AHSEPPDFDAFPWPSRHLLDNSRYYSFISQKRNFTIALTTLGCP
                     HTCNFCAITRMPRGVRSVDNVEAEIREVYDRYGVREIDYFDASFLTSSSRTSRLLEAM
                     ARAPSDMEYSIRARVDSIDAHTARALAHARVRQVYLGIESSNDAILSALHKHVQPSTA
                     RRAVALLQREGVRPLGFFMLGSPEETLVTAMRTILFAMSLGLDYAQFSRTIAKPNTPL
                     YDLLRTQTGRDYWRDYVRGTAPEERLPSPWTTLTETQIEAITKLAYFLFYYRPWYVVH
                     ALRRVRTREEFERSVRTAARMLLRSVYFD"
BASE COUNT          901 a         1123 c         1116 g          793 t
ORIGIN      
        1 cattcttgca actcatgcgt tgatgcgtga actacgccca aacatgatcc aagtggcctt
       61 tttcacaccc tatcccggcg cccctgttca tgaaagcgtg cgagagcggc atctcgaacc
      121 acaagccttc tcccattaca atcggctcat caacctctcg caggtctccg acgaagcgct
      181 tcgttcgtgg cagcgaaaac tgtattggga tttcctcatg aatcgacgat tcattgccga
      241 gtatcttcgt ttcgaaacga ttccggccct cgtcaacctc gatcaattcc tgccatttct
      301 tcgcttgagc gcgaagttcc ttacacggct caacaagagt caatgagctt gaagcacgga
      361 tgaaaggtcg cccgactctg acgatcgcac cctacgggat cgtcatgaac tcaaacaacc
      421 gagagagatc tcgatctttc cgttcttatc agcttgcacg gtggaacaga acgaagggca
      481 tagaatcaaa cgtgtcgggt tgttcttgtc gttgtaatac cacccttcac cagcacactg
      541 gctggcatca tcgacttgcg ggacgttccg aggagtccct ccgccgggcg taaaggtcaa
      601 ctgaaccaac gccggatcga tgactccggc atccgtggtg ggcatcacat actcacaacc
      661 caatgatttt tcgcggatat cgttgagggc ctggatgaat tgctgcgtca cgtttccacc
      721 cgtgtccacc aggtacgctt tctgggtgcc ccccgcggca gcgatcgcat tcatattcgc
      781 aagcgaagtc cccacaccga tgacgaacgt caaaaccgat ggcgtctgat tctttcccgc
      841 ggctgcgacg ttggatatct caggaatcgt cgatggtgtg caactatagg gatccccatc
      901 cgtggcgaga acgacgacga cggtatgccc ggggtgtgtg tttgcccaat tcgttgcgta
      961 ttgaatggcc ccggtgagtg ccgggcccgt gggcgttcct ccaaacggcg aggtggcatt
     1021 caaggaagac ttgatggctc ccgcgccacc gggaagcgtg gttatcccca ccgcgggcgt
     1081 ggaataatga gtcgtcgcgc aggctcgaca ataggtgttc caacaaggac cgtagttgcc
     1141 acactggccg tcgctcgagc aagtgctcgt atagcccgag ggaaggacag ggaagtactg
     1201 aagacctacg ccaattccgg cggaagcgct gtcatcgacg aatcctttga ccgcggctac
     1261 gacattgttc caacgagccc cctccatgga actggattta tccatcatga tatacatatc
     1321 cagaggggta agttgtgctt cgacgatggt ctcggcgcat gtggcgtcgg gattgaatcc
     1381 atcaggggtg ggaggggcat cataggaaaa atcgacatcc ggaccggcgt cgaacccagt
     1441 gccgccactg cccccggtgg cactggaacc agagcttccg ccttcaggcc attcgaatcc
     1501 cccggcacct cccgtcgaac ctgtcgatgc gccagctcct ccatccccgc cagagcctcc
     1561 tacgcctccc gtgcctccgg tcgcgggctt cgcatcccca accgaagcgt ctccagccgt
     1621 tgcaccatgg gatgacgcgt ccgaagagtc cgacgaacac gccatgaacc ccatggttcc
     1681 catcgcgagg gaagcgacga tgtacggaaa ccattttgcc atcctattaa gatgaagtgg
     1741 attgcggcga gggtcaacac catcgatttg gcttatcgaa atctgtccgg gacgatcctg
     1801 ccgccagccg catgaggcgc aagattcggc gaggagtcag atgtattcga acatacatga
     1861 tcgctcgcac cgttttccat gttcgtgatg gaacgacttg cccttggttc ggcaggtcaa
     1921 gcagcacgga tacatgcacc atgtcgaaag gaagggttcg cagcgacgat gcggactgaa
     1981 tcgcctgtcg ggcagattgg ccgtctatcc cgatcaggat atagcctgtc gtgacgatgt
     2041 tctttctttg cgcgtaggca acgatcggaa gaaatcgatc caggtcgaaa tggcgatgca
     2101 gcagaatacg atggttttcg tcgaggcttt cgatcccaaa tgcgatggat cgtacgccgg
     2161 ccgcagccat ggcatcgatc agttcctcat ccacgagagc gggatggtac ccgttgggaa
     2221 gatcgatccg aaatcgggga ttctgctcgg aaaaagccct ccataagcgc aaggcgtagt
     2281 ccctatccaa accgatgttg tcatcctcga gcacgagggt atccacgtgg tagtcgcggc
     2341 gaagcctttg cacctcctgc atgacttgct cgatgcttcg aagacggaac gtgccgcgag
     2401 acaaggcatt ggcattgcaa tacccgcacg acaaagggca gcctcgcgtg acaattaggt
     2461 tcgtgcggcg cgaagagcca aatacgcccc gacgataata cgcttccagg gggaacaccg
     2521 agcgatcggc aaaaggcaat cggtcgaggt gaggttcgac cgatacgtga tcgtctggcg
     2581 tctgtccccg aagagtcacg ccaggcacat cgagcgagcc gttcagcaga gcggcaaaag
     2641 gcgtctcccc atcgcctcga acgatggcat ccgcgcgggt tgcctgaagc gtttgtcgtg
     2701 gcgcaagcgt ggcatgggga cctcccacca caacgaagga ggcgggttgg attcgtcgca
     2761 cttgttgaac gatgaatgct gcttccgaaa gcgtggtgga aagcgcggtg atgcccacgt
     2821 aatcgaaggg atgttggcgc agcagttcga cgagttttgc tggcggatcg atgcgcaaat
     2881 cacaaccaag gaccaacgct cccgcgcgtt tggctgccgc ggctagataa gcaagcccca
     2941 aaggataggt gcgttccctc ggacgagaag gatgtaccag caacacgcaa cgtcgcaaag
     3001 ccgccatgag ccctcaatcg aagtacaccg aacgcaggag cattcgcgcg gcagttcgca
     3061 ccgatcgttc gaattcctca cgggtccgaa ctcgacgcag ggcatgaacg acgtaccagg
     3121 gacgataata aaacagaaaa taggcgagct tggtgatcgc ctcgatctgc gtttccgtca
     3181 gcgtggtcca ggggcttggc aaccgctctt cgggcgctgt tccgcgaacg taatcgcgcc
     3241 agtaatcccg gccggtttgg gtgcgcagca gatcgtagag cggtgtgttg ggtttcgcaa
     3301 tcgtccgaga aaactgcgca taatcaagcc ccaacgacat cgcgaacaag atcgttcgca
     3361 tcgccgtgac aagcgtttcc tcaggactgc cgagcatgaa aaatcccaac ggacgaaccc
     3421 cctcccgttg caacaaagcc accgctcgtc gtgccgtact cggttgcacg tgcttgtgca
     3481 acgcggacaa tatagcgtca ttcgacgatt cgatgccgag atatacttgc cgaacacgcg
     3541 cgtgggcaag cgcacgagca gtatgagcat cgatgctgtc gacccgtgcc cgaatcgaat
     3601 attccatatc ggatggcgcg cgagccatgg cctcgagcaa tcgtgaggtt cgcgacgatg
     3661 aggtgagaaa acttgcatcg aaatagtcga tctcgcgaac accgtagcgg tcgtatacct
     3721 cgcgtatctc cgcttcgaca ttgtcgacgg aacgtacacc gcgaggcatg cgggttatcg
     3781 cgcagaagtt gcaggtgtga gggcaaccaa gcgtggtcag agcaatggtg aagttgcgct
     3841 tctgcgagat gaaagaatag taccgcgagt tgtccaaaag atgacgagac ggccacggaa
     3901 acgcatcgaa atcgggtggt tcggaatgcg cag
//
LOCUS       DAOSOR010000549         3918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_23247_length_3918_cov_4.381828, whole genome shotgun sequence.
ACCESSION   DAOSOR010000549 DAOSOR010000000
VERSION     DAOSOR010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23247_length_3918_cov_4.381828"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2284
                     /locus_tag="PK710_21435"
     CDS             <1..2284
                     /locus_tag="PK710_21435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12356.1"
                     /translation="IFHFRVKMTSAGAVLGAPALYNLTATPADEGPIEVVGQRVAFTV
                     RMMSGTIGVRVLDLRGEAPLEGDWSTIARWQNAVTNLQQTGLTTGVNSVFWAIDPPKD
                     DVSLTLMLDGWIEMRSNGSIARMPTEGGRVVGDMTDLEYRPSTKGRPGNLVTWAVDRV
                     RAMPWFGDDRMQMLKTVAFGVLDVYRRAQTTVMGDSSAEEIAEDLGGIVARNAPTTFT
                     DPETGWPPAPIEPFLGKKMEGEGQWIPLENDPFIRMNPGVPPAFLTSFVRTDPKRSYT
                     RIYVTLWDPRQVELHMMAGSVEPKGASGEAGPGMIPRRPELMKRLVAGINGGFQALHG
                     EWGMMGDGVIYLPPKPFAATVAEMRDGFTGFGTWPNDPVVPPTIQSYRQNLTPLVMDE
                     VINPYKRGWWGGTPPEWEDRVHTTRTGICLTKEGFIAYLYGNEIDVMPLAQAMVQARC
                     QYGIHLDMNPGHTGLEYYKVAPTGTLPDLGRPLDKKWEAEGPVPFMEGWSFRARRMVR
                     FMGLMNFPRYIQRETRDYFYLTLRSVLPGPELDIAEGVQAQWRTSGLPQHGFPYAIAT
                     TELQPDPSRADTKVFIMKLDPRTVRLASEADDKLVVSMANVEVEPNQPTLWISDRACA
                     IASEPVNGAVAVVSGLEPGSLAGQEPVAALGIQDDDGMLVYIEIQQGKRPGADEAMLT
                     SLLEKLGCSSRLLLSRPLAPSLGHPASGGGRKVHFVRVDAPGARRIFESTPVVDPSEW
                     APLQARRIRYFKKPKASGNE"
     gene            2321..>3918
                     /locus_tag="PK710_21440"
     CDS             2321..>3918
                     /locus_tag="PK710_21440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12357.1"
                     /translation="MVSNPEYRKRFVLLSVLVLVAFLPSLMTGFVFDDIPLIVDNPYA
                     QSLVYLKRCFTTDLWDIPSRPDPEQWSLFYRPIISASYLLNWVASGGAAWSFHLVNVL
                     AHLGAVWLAFRTATRWLADVRLGFAAALVFGLHPTRNESVLWVSGRTDVFMALFLLLA
                     LELFSSASRRKGSRAVLTWIAGVLALLLALLSKEYAVVFPILVAVDAWVAKDAVYRRR
                     MWGASAGAIVCCAVFLWLRTWLMPLQPPDDPISFFLHAKYVFLSLGRYGSRLVFPWPQ
                     AIFVKPIFIENGEVVLPSFDIAVGVVSTLAFGAVLIWSMVRKRDRVPFLLCALALLLP
                     ILNIVYSGLNSTTADRYFYFPLFGIVVAGLSFAHPLLDRIQQITRGMLLGGLGIVCIA
                     ITWVRTLDFTTENAFWAQEIAVDPDNPLALFALATLAEKQGDRASYRKHLLRAYSPAS
                     TKYLLISTPARTYVRLVDDLLHTRPDGAREHLETLLSLLMALTEGRPIQHASAMEGIS
                     LEPPIIDQNLAVWLRAARPGLLTQA"
BASE COUNT          752 a          958 c         1248 g          960 t
ORIGIN      
        1 tatctttcat tttcgggtga agatgacttc ggcaggagcg gtgcttggcg cgccggctct
       61 ttacaacttg acggctacgc ctgcggatga aggtcccatc gaggtcgtgg gtcagagggt
      121 agcgtttacg gttcgcatga tgtcgggaac gattggtgta cgcgtgcttg atctgcgtgg
      181 cgaggcgccg ctcgaagggg attggtccac catcgcgcgc tggcaaaacg cggtgaccaa
      241 tcttcagcaa acagggttaa ctactggagt taactcagtt ttttgggcta tcgatccacc
      301 caaagacgac gtctcgttga cgctcatgct ggatggttgg atcgagatgc gttcgaatgg
      361 gagcatcgcg cgtatgccaa cggaaggtgg tcgtgttgtg ggagacatga ccgacctcga
      421 gtatcggcct tccacgaaag gtcgtcctgg aaacctggtg acgtgggcgg tcgaccgggt
      481 gcgagcgatg ccgtggtttg gcgacgatcg catgcagatg ttgaagacgg tggcattcgg
      541 tgttttggat gtttatcgtc gtgcgcagac gaccgtcatg ggggatagtt ccgcggagga
      601 gattgcggaa gatcttgggg gaattgtggc gcgaaacgcg ccgaccacgt ttacggatcc
      661 ggagacgggc tggccaccgg cgcccatcga accatttttg ggtaagaaga tggaaggaga
      721 gggacagtgg atacctctcg agaacgatcc tttcatccgc atgaatccag gcgtgccgcc
      781 agcatttctg acatcgtttg tgagaacgga tccgaagcgc agttatacgc gtatttacgt
      841 aaccttatgg gatccgcggc aggttgagct tcacatgatg gcagggtcgg tggaacccaa
      901 gggagcgtcg ggagaagcgg gacccgggat gattcctcgt cggcccgaac tgatgaaacg
      961 gctggtggcg ggcatcaatg gtggctttca agcgttgcat ggcgaatggg gaatgatggg
     1021 ggacggtgtg atttatcttc cgcccaagcc atttgccgcg acggtggcgg agatgcgtga
     1081 tggtttcacg gggtttggaa catggcccaa cgacccggtg gttccgccga ccatccaatc
     1141 ctatcgtcag aatctcacgc cgctggtgat ggacgaggtg atcaatccct acaagcgagg
     1201 gtggtgggga ggaacgccac cggagtggga agaccgcgtt cacacgacac gaacgggaat
     1261 ctgtctcacg aaagaagggt tcatcgcgta tttgtacggc aatgagatcg acgtgatgcc
     1321 gttggcgcag gcgatggttc aggcgagatg tcaatacggc atacacctgg acatgaaccc
     1381 gggacatacg gggttggagt attacaaggt tgcgccgacg gggacgttac cggatcttgg
     1441 gcgtccgttg gacaagaaat gggaagcgga ggggccagtg cccttcatgg aagggtggtc
     1501 gtttcgagcg cggcgcatgg tgcggttcat ggggctgatg aactttcccc gctatattca
     1561 gcgagaaacg cgagattatt tttatcttac gctccggtcg gtgcttccgg ggccagagct
     1621 ggacatcgcg gaaggggtgc aagcccaatg gcgtacgtct ggtctgccgc aacacggttt
     1681 tccgtatgcg attgccacaa cggagttgca gccggatccg tctcgtgctg acaccaaagt
     1741 attcatcatg aagcttgatc cgcgcacggt gcgtcttgca tcggaagcgg atgacaagct
     1801 ggtggtttcg atggcgaacg tggaagtgga gccgaaccaa ccgacgttgt ggatatcgga
     1861 tcgcgcgtgt gcgattgcat cggagccggt gaacggtgcg gtggcggtgg tatcggggct
     1921 ggagccaggt tcgctggcgg ggcaagagcc ggtggcggcg ctagggatcc aggatgatga
     1981 tggaatgctc gtgtacatcg agattcagca aggaaagcgg cctggggcgg acgaggcgat
     2041 gttgacgagt ttgttggaaa aattggggtg ttcgtcgcgt ttgttgttga gtcggccgtt
     2101 agctccttcg ttgggtcatc cagccagcgg aggaggtcgg aaggttcatt tcgttcgtgt
     2161 cgatgcgccg ggagcgcgga ggattttcga aagcacgccg gtggttgatc catccgaatg
     2221 ggctcccttg caagcgcgtc gcatacgata cttcaagaag ccgaaagcaa gcggtaacga
     2281 gtaactgttt tcggacctgc ggaggtggta tcgaaagtac atggtgtcga accccgagta
     2341 tcgcaaacgg ttcgtactgc tttcggtgct cgttctcgta gcgtttctgc caagcttgat
     2401 gactgggttc gtattcgatg acataccgct catcgtcgac aacccgtacg cgcagagcct
     2461 ggtgtatctc aaacggtgtt tcaccacgga tctttgggat attccttcga gaccggatcc
     2521 tgagcagtgg tcacttttct atcggccgat catctcagcc agttatctat tgaattgggt
     2581 agcatccggt ggtgcggcct ggtcgtttca tctggtcaat gtgttggctc atctgggtgc
     2641 cgtatggctt gcattcagga cggccacacg atggcttgcc gatgttcggc ttggatttgc
     2701 cgcggcgctc gtgtttggac ttcatccgac acgcaatgag tctgtccttt gggtttccgg
     2761 tcgcacggac gtattcatgg cgctgtttct gctgttggcg ctcgagcttt tttcttccgc
     2821 ttcgcggcgt aagggaagtc gtgcggtgtt gacgtggata gccggggtgt tggcattatt
     2881 gcttgcgctt ctttccaaag agtacgcggt ggtatttccg atcctcgtgg ctgtggatgc
     2941 atgggtggcg aaggacgctg tctatcgacg aagaatgtgg ggagcttcgg caggggcaat
     3001 cgtttgttgc gcggtgtttt tgtggttgag aacgtggctc atgccactcc aaccgcccga
     3061 tgatccgatt tcgttttttc ttcacgcgaa gtacgtattt ctatcgttgg ggcggtatgg
     3121 ttcgagactc gtgtttccat ggccgcaagc gattttcgtc aagccgattt tcattgaaaa
     3181 cggtgaggtc gtcttgccgt ctttcgacat cgcagtgggg gttgtatcga ccctcgcttt
     3241 tggggctgtc cttatctggt ccatggtacg aaaacgtgat cgagttccct ttctgttatg
     3301 cgctctcgcg ctgcttctcc cgatattgaa catcgtttat tcagggttga attcaaccac
     3361 cgcggaccgg tacttctact tcccactttt tggaatcgtc gtggccgggt tgagttttgc
     3421 acatcccctg ctcgaccgga ttcaacagat cactcgtggg atgttgctag gcggtttggg
     3481 cattgtgtgc atcgccatca cgtgggtgcg cacgctcgac ttcacgacgg aaaacgcgtt
     3541 ttgggctcaa gaaattgcgg ttgaccctga caatcccctg gcgctttttg ctcttgccac
     3601 actcgccgaa aagcaagggg accgcgcttc ctatcggaag catctacttc gtgcgtactc
     3661 acccgcgagt acgaaatact tactcatttc gacacccgcg cgaacgtatg tgcgtcttgt
     3721 cgacgacctg cttcacactc gtccagacgg agcgcgagag catctggaaa cgctcctatc
     3781 gttattgatg gcccttaccg aagggcgtcc cattcagcat gcgtcggcca tggaagggat
     3841 atctctcgaa ccgccaatca tcgatcaaaa tcttgcggtc tggctacgcg ctgcccggcc
     3901 ggggctcctt acgcaggc
//
LOCUS       DAOSOR010000550         3917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_23260_length_3917_cov_4.266960, whole genome shotgun sequence.
ACCESSION   DAOSOR010000550 DAOSOR010000000
VERSION     DAOSOR010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23260_length_3917_cov_4.266960"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3917)
                     /locus_tag="PK710_21445"
     CDS             complement(<1..>3917)
                     /locus_tag="PK710_21445"
                     /inference="COORDINATES: protein motif:HMM:NF024593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4011 domain-containing protein"
                     /protein_id="HOT12358.1"
                     /translation="VSSEDATLESAQLIVQVHDAITPLSHPVTRDIHLEPLQQVEFTD
                     FINILAPEKMRQVEETRQGEVAVWLVKGQQTLATQRLPLTVLAHSHWLGSPFGLAMEM
                     LSAHVMPNAPEIASLMVRVADYLQQFTSDSSIDGYQSGDPQRVDQIVAASYAAMAEKG
                     INYANPPASWEQAGQKVRTPAEVLGGRLGTCLDLSVVLAAVLEQCGLHPLLLMLEEHA
                     LVGYWREEVNSGSAFLAESGDLVNRVDLGMIGVVETTCIPHKTPFFEAVAEAKTRIQD
                     GLLGASDIVAARQLGIRPLPAISHGNGVIQVHEYKPAVHSRPRLAEVPAPLSRTTHPR
                     KAEKEPPTRVRHWKNRLLDLSLRNRLIHFTPRSSIELILSADDLPLVEDLLNAGHALP
                     LHPADAIDDTIKQRHDADSVKDMPLSVCSEAFKSRKILYGDISSESYQNELQKVAYRA
                     RSLEEETGANNLYITLGLLEWKLKERVLLSPLILVPVRLVASRRRGGTYSIVIDEAGE
                     STPNYCLLEKLRLEFGIEIPGLQQPVEDASGIDLQAAFDAVRQVSLETKADFQVRPVA
                     YLGSLQFNKFRLWKDLDEHWEKILQAPLARHLALHPTEPFVDPVKAPSHLDLDELGNT
                     CPIPADGSQLKAIGHALAGRTFVLEGPPGTGKSQTISNLLAQAIFQGKRVLFVAEKRV
                     ALEVVSNRVRELGLGEFMLDLHDQKSKPSAVQSQVRAALESSSESRGDPIGLSNAMET
                     LKSAQSILAQYARRLHARNSAGYSLYSATEACIAADPSVRAMPIPEHLLQAPAQVIQR
                     LEEVLRWLPAVADPVFPSPHAPWRFLEPRASGDINRAIVVEAARYLHPSAVALASGPA
                     VVLAIGNAAEAMLTAELVGYTIGPDTLQVLAAPSWQAAVNALGERVRQITVQQAAWPG
                     LREDFWDAAVAEILGLGGEIRNAGFFSRRGLLQQLCARLRACGMQTPDLDKDAAEVLI
                     GQLGAVKNASMAIASELNRIPTMSLGAGWTPLRQAHREAMSYRFGLMQRLVLRLGQGG
                     AFALRLREWLASGARLSPEHHGALRNLSWALGRLGGVVHPAAMQAWVGERGFVGRWLD
                     SSAHRRMDDPNLGSLSQWLALVDHLTPLVEAGMTSARAAILDGAISSNDAVRAWKRGL
                     EMASRSERSRTAGLEVFNAQAHNRSVERFTDSQDKSRALLAEVMAGKVVEKRSFDGNA
                     QAGRIGALKRELNKQRRRKSVRDLMSEYGDLITEAMPVVLVSPDSLSRFFSLDGPLFD
                     IIVFDEASQVRVSDAIGAIGRARSVVVVGDSKQMPPTSFLQTE"
BASE COUNT          862 a         1187 c         1105 g          763 t
ORIGIN      
        1 cactcggttt gcaaaaaact ggtgggcggc atttgcttcg aatcgcccac cacgaccacc
       61 gaacgcgccc ggccaatggc accaatcgca tccgagacgc gcacctggga agcctcgtca
      121 aaaacgatga tgtcgaataa cgggccatcg agagagaaaa atcgggataa tgaatccgga
      181 ctgaccagca ccacgggcat ggcttccgtg atgagatctc cgtactcgct catcaggtcc
      241 cgaacacttt tccttcgtcg ttgcttgttc aattctcgct tgagggcacc gatgcgaccg
      301 gcctgcgcat ttccgtcaaa gctgcgtttt tccacaactt ttcctgccat tacctcagcc
      361 agcagggcgc ggctcttgtc ctggctgtcc gtgaaacgct cgacgctgcg gttatgggct
      421 tgcgcgttga aaacctcgag acctgccgtg cgcgatcttt cgcttcgcga cgccatctcg
      481 agcccgcgct tccacgcacg aaccgcgtcg ttcgaagaaa tcgcgccatc gagaatcgcc
      541 gcccgggcgc tcgtcattcc cgcctcaaca aggggtgtaa gatggtccac caaagcaagc
      601 cactgcgaaa gagaaccgag attggggtca tccatccgac ggtgcgcgga gctgtcgagc
      661 caccgaccca caaacccccg ttccccgacc caggcctgca tcgccgcggg atgaacaacg
      721 ccgccaagtc ttccgagcgc ccacgaaagg tttcttagcg ctccatgatg ctccggcgaa
      781 agccgtgccc ccgaagccaa ccactcccga agccgcaacg caaacgcgcc gccttgccca
      841 aggcgtaaca ctaaacgttg catcaaccca aaccggtacg acatcgcttc ccgatgggcc
      901 tgccgcaacg gagtccaacc tgcgcccaac gacatggtcg gtatccgatt caactcggaa
      961 gcgatggcca tcgacgcgtt cttcaccgcc cccaactgac cgataagaac ctccgcggca
     1021 tccttgtcga gatccggtgt ctgcattccg caggcacgaa gtcttgcgca aagctgttgg
     1081 agcaaacccc gtcggctgaa aaaacccgcg ttccgaatct ccccccccaa gccgagaatc
     1141 tccgccacgg cagcgtccca aaaatcctcc cgcaaccccg gccacgccgc ctgctgaaca
     1201 gtgatctggc gtactcgttc ccccaacgcg ttgaccgccg cttgccatga gggcgccgcc
     1261 aatacctgaa gcgtatccgg accgatggta taacccacga gttccgccgt cagcatcgcc
     1321 tcggccgcat tgcctatcgc cagaaccacg gctggtcccg acgcaagggc caccgcggag
     1381 ggatgaagat aacgggcggc ttctacgaca atagcccgat tgatgtcccc actggcacgc
     1441 ggttccagaa accgccacgg tgcatgcggg gatgggaaaa ccgggtctgc caccgccgga
     1501 agccaacgca atacctcttc caatcgctgg atgacctggg caggggcttg cagcaaatgc
     1561 tccgggatgg gcatggcccg cacgcttgga tccgcggcaa tgcacgcttc cgtggcgcta
     1621 tacagggaat atcccgcgct attgcgggca tgaagccgcc gcgcgtactg cgcaaggatg
     1681 gattgtgcgg acttgagggt ttccatggcg ttggaaaggc caatggggtc cccccgggat
     1741 tcggatgagg attccaatgc cgcgcgcacc tgggattgaa ccgccgaggg cttgctcttc
     1801 tgatcgtgaa gatcaagcat gaactccccg agccccaatt ccctgactcg attcgaaacc
     1861 acttcgaggg ccacccgctt ttccgccaca aatagcactc ttttcccttg aaatatcgct
     1921 tgtgccagaa gattggagat ggtttgggat ttgcccgttc ccggaggccc ttcgagaacg
     1981 aaagtccgtc ccgcgagcgc atgccctatc gccttcaact gtgaaccatc cgcgggaatc
     2041 ggacaggtat tgccaagttc gtccaggtcc aaatgcgaag gagcctttac gggatccacg
     2101 aacggttccg tcggatgaag cgccagatgc cgagccaaag gcgcctgcaa tatcttctcc
     2161 caatgctcgt cgaggtcttt ccaaagacga aacttattga actgcaagga accaagatag
     2221 gcgacggggc ggacttgaaa gtccgctttg gtttcgaggg aaacctggcg aacggcatca
     2281 aaagcggcct gaagatcgat gccggaagcg tcttccacgg gctgctgaag acccggaatc
     2341 tcgatgccga attccagacg aagtttctct aatagacaat agttcggggt cgactccccc
     2401 gcttcgtcaa tgacgatact gtaggtcccc ccccggcgtc gtgaagccac gaggcggacg
     2461 ggcacgagga tcaggggcga caagagcacg cgctctttga gcttccattc gagcaggccg
     2521 agggtaatat acaggttatt ggcacccgtc tcttcctcga ggctgcgagc ccgatacgca
     2581 actttctgaa gctcgttttg atacgattcg gatgaaatat ctccgtacag gatttttctg
     2641 ctcttgaagg cttcggaaca gaccgaaaga ggcatgtctt tcaccgaatc cgcatcatga
     2701 cgctgtttga tggtgtcatc gatggcgtcg gccggatgca agggaagagc gtgcccggcg
     2761 ttgagcagat cttcgactag cggcagatca tcggcagaaa gaatgagttc gatagacgag
     2821 cgaggcgtaa agtgaatgag tcggttgcgc aaagaaaggt cgagaagacg gtttttccag
     2881 tggcgtacgc gcgttggggg ctctttttcc gctttgcgag ggtgggttgt gcgggacagg
     2941 ggcgcaggca cctcggcaag ccttgggcga gaatgtacag cgggcttgta ttcatggacc
     3001 tgaatgacac cgttaccatg ggatatggca ggcaaagggc gtatgccaag ttgacgggcc
     3061 gcgacaatat cgctggctcc gagaagaccg tcctgaatac gcgttttggc ttccgcgaca
     3121 gcctcgaaaa acggggtttt atgagggata cacgttgtct ctaccacgcc gatcatgcca
     3181 agatccacgc ggttcaccag atccccggat tcggccaaaa aagcgctgcc cgaattcact
     3241 tcttcacgcc aatagcccac gagggcatgc tcctcgagca tcagcaaaag aggatgcaaa
     3301 ccgcattgtt cgagcacggc agccagcacc acgctcaggt ccaggcaagt gcccaatctt
     3361 cccccaagaa cttccgcggg agttcgtact ttctgccccg cctgttccca actcgccgga
     3421 ggattggcgt agttgattcc cttttctgcc atggccgcgt acgacgccgc aacgatctga
     3481 tccacgcgct gaggatcacc ggactgatac ccatcgatgc tggagtcgga tgtaaattgt
     3541 tggagatagt cggccacacg aaccatgagc gaggcgattt ccggggcatt gggcatcacg
     3601 tgggcggaaa gcatttccat ggcgagccca aacgggctcc ccagccaatg gctatgagcc
     3661 aatacggtca gaggcaagcg ttgggtggca agggtttgct ggcctttcac cagccacacg
     3721 gcgacctccc cctggcgtgt ttcctccacc tgacgcatct tttccggggc gaggatgttg
     3781 ataaagtccg taaattcaac ctgttggaga ggttcgaggt ggatgtcgcg cgtgaccgga
     3841 tgggaaagag gcgtgatggc gtcgtgaacc tgcacgatga gttgggcaga ctcgagcgtg
     3901 gcgtcttcgc tcgacac
//
LOCUS       DAOSOR010000551         3904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_23365_length_3904_cov_5.001039, whole genome shotgun sequence.
ACCESSION   DAOSOR010000551 DAOSOR010000000
VERSION     DAOSOR010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23365_length_3904_cov_5.001039"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(523..1680)
                     /locus_tag="PK710_21450"
     CDS             complement(523..1680)
                     /locus_tag="PK710_21450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12359.1"
                     /translation="MESSSVLERAGGCHRRGLSWLRRGFRVSYGLSVMSPTVIYPEFH
                     MPPVPSLIASYGVCGVLPLLLASFAALNWARDRLRARRAKAATESTAPAADGQTVVHG
                     TVELAQGATHAARVEIEQFGFESCYKHSWSHEWKELERRMLVEPFYVREPNGRRIRVE
                     ATTDAYIIDELDEMIQTARDKRVRAATVNPGEEVYVFGVLGFGKDPEAASSYRDDGHG
                     LVLRSPDAGHMLLATTSPAARFRASTRVHGLWVVIFILLAGVFQIMNVMHSVRVAAGQ
                     TATATVLGTRTYTTEGKKAYFTITSWSRVAFPMARCSKKRSRRRTGRNCPKGQKSPSS
                     TCPANLDSKFSAIAPPYTLLPGSCRSSRACSSSSATWAIERRHDPGTSAPS"
     gene            2320..2568
                     /locus_tag="PK710_21455"
     CDS             2320..2568
                     /locus_tag="PK710_21455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12360.1"
                     /translation="MQKRFTAQGLISFFPTMHGGSADRVDDLEFDQQNTHRTEWTAKT
                     PSKGWVVQGIDDVGMERNRLRIHRLSTLPQGCSSVVLC"
BASE COUNT          843 a         1136 c         1153 g          772 t
ORIGIN      
        1 cggatcacga tcagctacaa aaatcgatac gtacgacttc tgggatgaga actaattcac
       61 ggccccttac taccccatag cggagtctga tggctatcac acttccttcg cgatagatac
      121 agccgaagca actccctttt ggaaatacgg caaagcgaat caggtcagga tccgagccat
      181 cccctcattt ttcccgggga atttcaatat ctcgccaatc gcgcatgtgc gggcgggttt
      241 gaaacccgcc cctacggttc cggttgttgc cctgagccat ccccttatta ttccccaagc
      301 ctttccttca cgatcacgat cacggccgac gataacggcc gctgtcacac aaaaccagag
      361 cggcgaccca ggccacgatc gctgggttca ggcacttctt tcacttcggt ttccaggttc
      421 gaggccgttg gctacgtgtc gcaatccgtt cgatggagga cgagggagcc aagaaaggcg
      481 caacgtttta tggatccggc agcttgccct ttccacggtc tttcaagatg gcgcgctcgt
      541 accagggtcg tgccgccttt cgatggccca ggtagccgag gaagaagaac acgcacgcga
      601 agaacggcac gagcccggca gcaaggtgta cggtggggcg atcgccgaga atttcgaatc
      661 caggtttgcc gggcacgtag acgacggcga cttctgaccc ttgggacagt ttcgcccagt
      721 ccgtctcctc gaccttttct tcgaacaccg tgccatcgga aacgcgactc ttgaccacga
      781 agtgatggta aaatacgctt tttttccctc ggtggtgtag gtgcgtgttc cgaggaccgt
      841 agcggtggcg gtctggccgg cagccacgcg cacggagtgc atcacgttca tgatttggaa
      901 aacaccggca agcaggatga aaatgacgac ccaaaggccg tgaacccgcg tgctggcacg
      961 gaagcgggca gcgggtgaag tcgtcgcaag aagcatgtgt cccgcatcgg gtgagcgcag
     1021 gaccagacca tgcccatcat cgcggtacga gctggccgcc tccgggtcct ttccgaagcc
     1081 cagcacaccg aaaacataga cctcttcgcc agggttgacc gtcgcggcgc ggactcgctt
     1141 gtcacgggcg gtttgaatca tttcatctag ctcgtcgatg atgtacgcgt cggtggtcgc
     1201 ttcgacacgg atgcggcgcc cgttcggctc tcgtacgtag aaaggctcga cgagcatcct
     1261 gcgttcgagt tccttccact cgtgactcca actgtgtttg tagcaggatt cgaatccgaa
     1321 ttgctcgatt tcgacgcggg ctgcgtgggt agccccttgg gcgagttcga cggtgccgtg
     1381 aacgactgtt tgtccgtcgg cggcaggagc cgtggattcg gtggccgctt tggcgcgacg
     1441 ggcgcggagg cggtcgcgag cccagttcaa ggcggcaaag gatgccaata ggagggggag
     1501 aaccccgcag acgccgtagc tggcgatcag cgagggaacg ggaggcatgt gaaactcggg
     1561 gtagatgacg gtgggggaca tgaccgatag tccataggaa acccgaaaac ctctccgaag
     1621 ccaactcaaa cctcgacgat ggcagccgcc tgcgcgttcg agcacggagg acgactccac
     1681 cagccatcca tggggcgaag ggggatctcg atacaaacca atgggttgac gcgcttgccc
     1741 ccagcgaacg acgccacagc cctccccgac cagcagctac gccgcggaca agaccgggtc
     1801 gaaacccagt tgtggattac gaatgctccg tttcgtttga cgtgatggga cgatgctgag
     1861 ccagcttccg tgtttcggcg tgtggtgtat ctcgaatcgc aggcttcgcg gacgcgcctg
     1921 ctccggcgca aacgcctcgt agaaactgac gggagtgcgg gtgtcaaggt tgtagcagac
     1981 cagcacgatc ttggtcgcct gtggatacac ctcgtcgcca ggtagcgtag aaattcggcg
     2041 ccatcgactg ctgtacgtct gtgggtaggc tgcaagatga cctcgccggt cagcggttcg
     2101 acggcgcgga ataggttggc ggtaccgcac cgcttgtact cgtcgtcaac gcgctcagcc
     2161 attggaggcc gcacaggcaa tgccggtcgc gcgtgcagga ccagttgctt gctcgtttcg
     2221 tcgaagcagg ccacgggccg ctgtgggtcg tagggcaggg ggaacacatc cagcacgtcc
     2281 tgcatcgact gcacgaacgc agcgttgtgc atgggcggaa tgcagaagcg tttcaccgcc
     2341 cagggcttga tttcattttt tccgacgatg cacggtggtt ccgctgatcg tgtcgacgat
     2401 ctcgaattcg accaacagaa cactcatcgg acggaatgga cagcgaaaac gccatcaaag
     2461 ggatgggtcg tgcagggtat cgatgacgtg ggcatggagc gtaatcgctt gcggatccat
     2521 cgtttgtcca cactaccgca agggtgctcc agtgtcgtgc tttgctaagg gattggggca
     2581 atcatggctc acgcgcgata gtcacggtgg ctctcagcat gaaataccca cggtgaccgg
     2641 gaccgaggcg acgggtctgc aaaggaagca gagcactttc caaacaccac tgatgccgca
     2701 acgatcctgc gccgtgtgtg attcgcgcgc aacgccacac acaggatgaa agatcatccc
     2761 atcgatccga cctatctcta attagagcga ggaaccgaat ctgggcaatt gtacgcagta
     2821 cctgtcaagg tgaccgtcgt ggttgtccca tccccttgac cgagctgagg ctcatcatga
     2881 cgcacgtggg tagcgcccaa cttggcggct ttgttccgaa gatcattcat tgcgtattca
     2941 atgagttttt cgttacttac catgcctccg ccaaaggttc ctcctcccgt tgcggtcacc
     3001 aaccccaagg ttttgcattc ggggggtagt gcgtcacgcg tgggtgcaac acgagcgcct
     3061 tgctctgtga gagatgctgt tttgcacccc gagacaatgc caagtaccaa aaaaccaagt
     3121 acagactatt ttaggatcga cacaagaaaa gcatagccga tccttctgcg gtgtatcaag
     3181 aacgaaaaga acaaaactat cgacctatga ggaggcttgg tcgaacgttg cactgcccgc
     3241 cttctgcggc gcacgggacg gcagcaactg ttcttccggt cgtttttccc cttgtccagc
     3301 ccaagagcag gacatgcgcc cgattcgggt tcgttggtca tgggacaatc gcgcgtagct
     3361 gtcatcatcc acaaggccat gatcgacatt ccgccaccct ttgccggccg caccccggta
     3421 tcgctgggac cgcgggcatc tcgcccacac gctacattcg cgaccatgcc caactgcgta
     3481 cggtcatcga ctacatcgag tccaatccgg tcaaggcggg gctcgtggag cgcaaacaag
     3541 actggcgata cggcagtgcg gatgacgatt gagacaatgt gcgcgagacc cactcggtcc
     3601 caggtacggc cggattgcaa ggtatgccga agacgtgctt ccgacagcga gccgggctgg
     3661 ggcgtgtcgt ccatcaactt cgacatcaac gcgtacctag cgcgagcaaa gaagccatga
     3721 aaagcgctac aaaatcacct aaatagctca ttcgacgtcg ggaagttgct gcctgggagt
     3781 cgaggagcaa caggaatcgg gtgcatcgaa aaaggacgcc ggcttggccg caagcgcatc
     3841 tcggagatcc gtgcccgacc acgccccgag gctcctcgga cttacccaag gcgtgcccga
     3901 ccac
//
LOCUS       DAOSOR010000552         3869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_23623_length_3869_cov_3.433403, whole genome shotgun sequence.
ACCESSION   DAOSOR010000552 DAOSOR010000000
VERSION     DAOSOR010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23623_length_3869_cov_3.433403"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..1406
                     /locus_tag="PK710_21460"
     CDS             201..1406
                     /locus_tag="PK710_21460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12361.1"
                     /translation="MKYLYGDSVPSPIRFDFLTAFETFVDVAVKAVRIEQEIGQAQAA
                     VDLAVEAREEALSIVESFHQKVMVYLDALLDEQQDPNTSVYVRQLREHGDKVVAEARN
                     NCRVAMELAVKERGAEFGARRNELMSLIERFLITELVTPVHVSSVMRLSEGQNQMTTI
                     MRHPGGIVATYSAVPVAGSVWQGPVRLASLVQGLDLQLGTRKSWITGSVAKSAVSLDD
                     FIVGGFELADESAEIRLRKRVDQADSFQFNISRQGEKLLVQVERKGEAEADAMLDLDE
                     GERLRIEKLWQMLRQSVRNTVRSRGGLLSVTMDGEDLWVGRGVRVFFNRIVEHLAPTV
                     TQIIQHSPNPAELSMKIENEEGRREEVYVRRQSLMAKIESLDDDGRSVFSPFGLAGDE
                     LTSEDVDIV"
     gene            1979..>3869
                     /locus_tag="PK710_21465"
     CDS             1979..>3869
                     /locus_tag="PK710_21465"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOT12362.1"
                     /translation="MPRASGLSFLIALSVLASSLTAQAITRCEVLRRAQAWVDDPNSW
                     YSWDATYTDPTTGVCCYRPDCSGMVSAAWGLPHPGHTTYSFAPGPWDDGVSYVISASQ
                     LKPGDALNYPGNPSAGSGHIMLYVSGNFNSGYVEVMEEYSYGHTATRRWRNIDTSIYY
                     PIRYKNIVDGEPEVCNGIDDDCDGLIDEGGVCDHEYLLNGQARVAPARTTDIDGDGRA
                     DVCGRGGAGVWCHLSNGTSWAVRGPVLGLSDDSGWGDSSNWGTFRFGDIDGDGRADLC
                     ARADKGMSCWKSDGVSLSKKVTGPELSDAKGFSKVEYFSTIRLLDMDGDGMDDVCVRA
                     AKGIVCYKSTGDGFGAVLNGPPWSDTAGLKEPKYYGTLQTGDVNGDGRMDVCMRIKEG
                     MECWLSDGNGFPTKVKGPEWSDAMGWGARQYWSTIRLVDVNGDGKADLCARNKSSLRC
                     HFSTGKGFEKAVEVAALSDEQGWNKEGNYLTLRTGDIDGDGAQDLCIRSNTKMLCYLW
                     NGSQFTRVEGPAWSNDQGWNNAEYYNTIQMGDFDGDGKADICGRHADGWRCHSSTGKG
                     FGNAVLLDEFKDKSGWKQSKYYATIFLGGPMCIPSDEICNGKDDDCDGLIDEDGVCDE
                     PGVPEV"
BASE COUNT          836 a          856 c         1272 g          905 t
ORIGIN      
        1 tcatgcggtg gttgggcgct gggtggggcg cgttgttggc gtgggtcgtt gccgtcctgt
       61 ttgtgacgat tcccacggtt ttgtggttat cgcatggagg acgaatgcga atgagtcgta
      121 tggcgcgttg agttgacgtt cgtttttgtc attatcagat tttcgagaga tactaacgat
      181 tcatcgaagg ggcatgacgc atgaagtact tatacggtga ctccgttccc tctccaattc
      241 gattcgattt tctcaccgct ttcgaaacgt ttgtagacgt cgcggtgaaa gccgttcgga
      301 tcgagcaaga aattggtcaa gcgcaggcgg ccgtcgatct ggcggtcgag gcacgagaag
      361 aggctttgag catcgtggag tcatttcatc aaaaggtcat ggtgtatttg gatgctcttc
      421 tcgacgagca gcaggatccg aacacttcgg tgtatgttcg gcaacttcgg gagcatgggg
      481 acaaagtggt ggcggaagct cgaaacaatt gtcgggttgc catggagctt gcggtcaagg
      541 agcgcggcgc cgaatttggg gcgcgacgca acgagcttat gtccttgatc gagcgttttc
      601 tcatcaccga gttggtgacg ccggtccatg tctccagtgt gatgcgcttg tcggaagggc
      661 agaatcagat gacgacgatc atgcgtcatc cgggggggat cgtcgccacg tattcggcgg
      721 taccggtggc tgggtctgtc tggcaagggc cggtgcgact ggcgtcgttg gtgcaaggtc
      781 tcgatcttca actcggtacg cgaaaaagct ggattacggg ttctgtggcg aaaagcgctg
      841 tttcgcttga tgacttcatc gtcggaggct ttgagcttgc ggacgagtcc gcggaaatac
      901 gtcttcgtaa acgggtcgat caggcggata gctttcagtt caacatctcg cgtcaagggg
      961 agaagttgct cgtgcaggtg gagcgcaagg gggaggcgga agcggatgcg atgctcgatc
     1021 tcgatgaagg cgagcgtctt cgcatcgaga aattatggca aatgttgcga cagtcggttc
     1081 gaaacaccgt ccgttcgcgg ggtgggcttt tgagtgtgac gatggacggt gaggatctat
     1141 gggttggccg tggtgtacgg gtatttttca atcgaatcgt cgagcacctg gcgcccacgg
     1201 tcacgcaaat catccagcac agtcctaacc ccgcggagct ttcgatgaag atcgaaaatg
     1261 aggaagggcg aagagaagag gtgtatgtgc gtcgacaatc tttgatggcg aaaatcgagt
     1321 cgctcgatga cgatgggagg tcggtttttt cgccgttcgg tttggctggc gacgaattga
     1381 catcggagga tgtggatatc gtatgaccgt tcgacctagg gggacccacg aaaaaaaact
     1441 aaaatagtct atattgtcaa taagttacgt ctatctggat cctgggtttc ggcagtggat
     1501 gcgagcatgc catggggggc gtggacgagt tgttgtgatg ggaggcaggc gccgtgcgtg
     1561 cttcggtgtt gatcatggcg ctcccggcag agcgatgagg gaatgatgag gaggtgatgg
     1621 aaagacgaac caaggccagt cgacgcatgc gcgggaaggt ggatacgagc acgatggctg
     1681 gttgtgcgga ggactacctc gaagtgcgga tcgttctcag gacttcggcc gatagtcttt
     1741 ggaatgatcg ctttggcgat catgcgcgca tccatcgtgg aaatggacaa gggcaattcg
     1801 gggcgaggat gcgctatcca tcgtttgctg cccatcaaac ggaggcaacg tggagcgaat
     1861 cttgctttgt atcccatcta tgggcattct tcgacgctat tctccacctt ccacagctat
     1921 ttcacccgcc aggaccatga ttcgggatcg cactgagatc gccaggatca tacccaggat
     1981 gcccagagcg tctggtctgt cctttctcat tgccctgtcc gtgctcgcat cgtccttgac
     2041 ggcccaggcc atcacgcgct gcgaggtatt gcgtcgcgcc caggcctggg tcgatgaccc
     2101 caattcatgg tattcctggg atgccacgta tacggatccg acgacgggtg tatgttgtta
     2161 tcggcccgat tgttcgggca tggtttcggc ggcctggggg cttccgcacc ccgggcatac
     2221 gacctatagt ttcgctccgg ggccctggga cgacggtgtc tcctacgtga taagcgccag
     2281 tcagctcaaa ccgggggatg cattgaatta tccaggcaat ccaagcgccg gttccggcca
     2341 tatcatgctt tacgtatcgg gtaatttcaa tagtggatat gtcgaggtga tggaggaata
     2401 ttcttacgga cacacagcta cacgacgctg gagaaatatc gatacatcga tctattatcc
     2461 aatcagatat aaaaatatcg tcgatggaga acccgaggtt tgtaacggga ttgacgatga
     2521 ttgtgatggc ttgatcgatg agggcggggt gtgtgatcat gaatacttgt tgaacgggca
     2581 ggcgagggtg gcaccggccc ggaccacgga tatcgatggg gatgggcgag cggatgtatg
     2641 tggacgtggg ggtgctgggg tatggtgcca cttgtccaac ggtacctcct gggcggtaag
     2701 ggggccggtt cttggcttgt cagatgatag cggatggggc gattcctcca attggggaac
     2761 ctttcgattc ggcgatatcg acggtgatgg tcgagcggat ttatgtgctc gtgctgataa
     2821 ggggatgagt tgttggaagt ccgatggcgt gagtctgagc aagaaagtga cgggccccga
     2881 gctttcggat gcgaagggat tcagcaaagt cgaatacttc agcacgatcc gccttctgga
     2941 tatggatggc gacggcatgg acgacgtatg tgtgcgagcg gcgaagggga tcgtatgcta
     3001 caagtccacg ggcgatggtt ttggagcggt gttgaacggt cctccgtggt cggataccgc
     3061 gggactcaag gagccgaagt attatggtac gctccaaacc ggggatgtga atggggatgg
     3121 gcgcatggac gtatgcatgc gcatcaaaga gggaatggaa tgttggcttt cggatggcaa
     3181 tggattccct accaaggtaa aaggaccgga atggtccgat gccatggggt ggggagcgcg
     3241 gcagtattgg agcacgatcc ggttggtaga cgtgaatggg gatggcaaag cagacctttg
     3301 tgcaaggaac aaaagttcct tgcgctgtca tttctccacg ggaaagggat ttgaaaaagc
     3361 cgtggaagtc gcggctttgt cggatgaaca aggctggaac aaggaaggaa actatcttac
     3421 tttgcgaacg ggagatatcg acggcgatgg ggcgcaggac ctttgcattc gctccaacac
     3481 gaaaatgcta tgctatttgt ggaatggctc ccagtttaca cgggtggaag gccccgcctg
     3541 gtccaatgac caaggttgga ataacgcgga gtattacaat accatccaga tgggggattt
     3601 cgatggcgat ggcaaagccg atatttgtgg tcgtcacgcg gacggatggc gctgccactc
     3661 ctccacgggc aaaggttttg gcaacgcggt gttgctcgat gaattcaaag acaaaagcgg
     3721 gtggaaacag tccaagtatt acgcaaccat ttttctgggc ggccccatgt gcattccgtc
     3781 cgacgagatc tgcaacggca aggatgacga ttgcgatggg ctgatcgatg aggacggggt
     3841 ttgtgatgag cctggcgtcc ccgaggtgg
//
LOCUS       DAOSOR010000553         3863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_23675_length_3863_cov_4.317489, whole genome shotgun sequence.
ACCESSION   DAOSOR010000553 DAOSOR010000000
VERSION     DAOSOR010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23675_length_3863_cov_4.317489"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="PK710_21470"
     CDS             <1..1244
                     /locus_tag="PK710_21470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT12363.1"
                     /translation="NMLENPAISGVVIVARDVTERRQMEARILQAQKLESLGVLAGGV
                     AHDFNNLLTSILGNTSLAAQTLGQDSSAQTLLDRVQTAARRAAELTRQLLTFSGHHSV
                     QGHPVHLGELTHEIAELMRVSIGRNRTLDLAMPEDLPAVLADATQLRQVVMNLIVNAA
                     ESYGDASGQVRVQIGERHCDDQCLRSCNFVSDQASGGHYVYVSVSDDGKGMASETVAR
                     IFDPFFTTKFTGRGLGLSSVLGTIRSHGGAICVQSKPGHGSTFTVLLPADKQKTPISG
                     LEARHNTPVVVPQQGTILVVDDEAVIRDFCVAGLKTAGLHALSASDGPSAIETLHTLG
                     DDLRLVLLDLTMPNTNTDQVIASIREHSPQLPILLTSGHAESHVLSRIKNLNETRFLP
                     KPFCLHELLDAVRDALQEPTG"
     gene            complement(1249..2769)
                     /locus_tag="PK710_21475"
     CDS             complement(1249..2769)
                     /locus_tag="PK710_21475"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT12364.1"
                     /translation="MTHKRWIGFIQAIGIAVSLCIGAQAVAAWDHPANRARILISELR
                     LKEAEPWLQGALSDPQAALEYGKLLLFSTRYEEAARILERPDVAAIAQGAQLGQLARD
                     SAQAVAGAFVVHDIHHDVIVRMQDDHDQPLVPFISEVIDASRSSLRQGLGVELPRPMY
                     VELVRDHFSLAKMTGLPEDAARTTGTVAIANWGRVTMVSPRAMSAGYPWMDTLAHELT
                     HIALGMGTRDRAPLWLQEGVAKTFEKRWRRPDDYDPYPSPHDIAAAGISKNLARDFDD
                     LGPSVALLPSPTHALVVYAQVESFLRFLLSELGEQMLVELIPRLREAGPSEGVSEVLA
                     SMTGRDLQAWQQAWRAWLTGTPRPVPSELSRERGPDALVLRDVRLGRLLLERGHGAAA
                     ARLLQTAEERWPNDLQIRYLLAKASLRHGDVDSAWNLLRQAGPPMAPHAEAFSLLGRM
                     REERHDGVGAEYAFFRGLSLNPWSSECACEAKPPPALPTDAARSWLCEAARNAPRY"
     gene            complement(2766..>3863)
                     /locus_tag="PK710_21480"
     CDS             complement(2766..>3863)
                     /locus_tag="PK710_21480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12365.1"
                     /translation="AALHLAARLRSPHPSLSGGGGRSGTESGGMPGLDDASAGDGDME
                     SFDSEGDEIEKLAKDHASRMEEVQQAIAEAMQSIDMQGLHDLAREHARIVRDSVRGLP
                     TSSSDMSSLEGAAMVLREQAERMAAALERTALSEADQAASNALKAAEQAVLLGQRERD
                     LFGQPSSLSREVVRARETLEREQRWVREQLDAMRKSASEQGKQRLKTESERQRELADR
                     AKDVLKRARSGQEPIPLSTVELIERAESHMREAARALDRADGDQAMTLQRMAQRWLEM
                     ASESMQDTANSNTPSPQTNPSHAESLEGQRDAHGEGDGKNIAKGPVDIPKAEAFTGPE
                     SFRRRVMDGLSHPADPSLREAVRRYSEGLLR"
BASE COUNT          762 a         1168 c         1115 g          818 t
ORIGIN      
        1 cgaatatgtt ggagaatcca gccatcagcg gggtggtcat cgtcgcccgg gacgtgacgg
       61 aacgccgtca gatggaggct cgaatccttc aggcgcagaa gctcgagagc ttgggggtat
      121 tggcgggagg cgttgcccat gatttcaaca atctgctgac atcgattctt ggcaacacga
      181 gcctcgcggc tcagacgctt gggcaagaca gttccgcgca gacactgctc gaccgggtgc
      241 aaaccgccgc gcgtcgtgcc gctgaattga cccgccaact tctaacgttt tccgggcatc
      301 attcggtgca gggtcaccct gtgcatttgg gtgagctaac ccatgaaatc gcggaactca
      361 tgcgtgtgag catcggccgc aatcgaacgt tggaccttgc tatgccagag gatctgcctg
      421 ctgtcctcgc cgatgccacc caattgcgtc aagtggtgat gaatctcatc gtgaatgcgg
      481 ctgagtctta cggcgacgca tcaggacagg tacgcgtgca gatcggtgag cgccattgcg
      541 atgaccaatg tctgcgctct tgcaacttcg tttcggacca agcctccggt ggacattacg
      601 tatatgtctc tgtgagcgac gatggcaaag gtatggcatc ggagacggtg gcacgtattt
      661 tcgacccgtt cttcaccacc aaattcacgg gccgcgggct tggattgtcg tctgtgctcg
      721 gcaccattcg tagtcatggt ggagcgattt gcgtccaatc caaacccgga cacggctcca
      781 cgtttacggt cctgcttcct gccgacaaac agaaaacacc aatttcaggc ctcgaagcca
      841 gacataacac gcccgttgtc gtaccacagc aaggtacgat tctcgtcgtc gacgacgaag
      901 cggtcattcg cgatttttgc gtagctggac tgaaaaccgc gggattacat gcgttgagcg
      961 cttccgatgg tccctctgcc atcgagacgc ttcacactct cggcgatgac ctccgtttgg
     1021 tattgctcga cttgaccatg cccaacacca acaccgatca ggtgattgca tcgattcggg
     1081 aacactcgcc acagcttccc atcctgttga cgagcggaca cgccgaatct catgttttgt
     1141 ctcgaatcaa gaatctaaac gaaacccgct ttctgcccaa gccgttctgt ttgcatgaac
     1201 tactcgatgc cgtgcgcgac gcattgcagg agcccacagg gtagcgcatc agtagcgtgg
     1261 ggcatttcgt gcggcttcgc aaagccagga acgggcggca tcggtgggca gagcaggagg
     1321 cggtttggct tcacaggcgc actccgaact ccagggattc aacgaaagtc cacgaaaaaa
     1381 ggcatattcc gcacctactc cgtcatggcg ctcttcccgc atccggccca gaagcgagaa
     1441 ggcttccgcg tggggggcca tggggggccc agcctgtcgt agcagattcc aagcgctatc
     1501 cacgtctcca tgacgaaggc ttgctttcgc tagcaagtac cggatttgca agtcattggg
     1561 ccatcgttcc tcggcagtct gaagcaaacg agccgccgca gcgccgtgtc ctctttccag
     1621 cagcagacgc cccaatcgca catcgcgaag cacgagcgca tccggtccgc gctcacggga
     1681 cagctcgctg ggcacgggtc gtggtgtccc tgttaaccac gctcgccatg cctgttgcca
     1741 agcttgtagg tcgcgcccgg tcatcgacgc gagcacttcc gaaacacctt ccgatggacc
     1801 tgcttcgcgc agccgaggaa tcagttccac caacatctgt tcaccgagtt cggatagcag
     1861 gaaccggaga aaactctcca cctgggcata gaccaccaag gcgtgggttg gggaaggcag
     1921 caacgccacg gaaggaccca ggtcatcgaa gtcccgtgcc aggttcttcg agattcccgc
     1981 agccgcaata tcatggggag aaggatatgg atcgtagtcg tcggggcgac gccaccgctt
     2041 ctcgaaggtt ttcgccactc cttcttgcaa ccacaaggga gcccgatccc gtgttcccat
     2101 ccccagcgca atatgagtaa gctcatgcgc aagggtatcc atccacggat agccagcgct
     2161 catcgcgcga ggagaaacca tcgttacccg tccccaattg gcgatggcca ccgttccggt
     2221 cgtgcgcgcg gcatcttccg gaagaccagt catttttgcc aaggaaaaat gatcgcgcac
     2281 gagttccaca tacatgggtc gcggaagctc cacaccaagt ccttgtcgca acgacgatcg
     2341 tgatgcatcg atgacttccg agatgaacgg gaccaagggc tggtcatggt catcctgcat
     2401 gcgcacgatg acgtcgtggt ggatatcatg cacgacgaag gcgcccgcga cggcctgagc
     2461 gctatctcgc gcgagttggc cgagttgtgc gccttgggca attgcggcga catcgggccg
     2521 ctccaggatg cgcgcggctt cttcgtaacg tgtggaaaac agtagtaatt tcccgtattc
     2581 gagagctgct tgcggatcgg acaaggcacc ttggagccat ggctccgcct ctttcagccg
     2641 gagttccgaa atgagaatgc gcgcccggtt cgcgggatga tcccaagccg ccacggcttg
     2701 cgctccgatg cataacgaca ccgcgatgcc aatcgcctgg atgaatccga tccaacgctt
     2761 gtgggtcatc gcaacaatcc ctccgagtat cgacgaactg cctcgcgaag ggatgggtcg
     2821 gcagggtgcg aaaggccgtc catcacacgt cgtcgaaagg attccggacc ggtgaaggct
     2881 tcggctttgg ggatatcgac aggtcctttg gcgatgtttt ttccatcccc ctcgccatgc
     2941 gcatcccgtt gaccttcgag gctttcggcg tgggacgggt tggtctgcgg ggagggtgta
     3001 ttggaattgg cggtatcctg catggattcg ctagccattt cgagccatcg ctgtgccatg
     3061 cgttgcaagg tcatggcctg gtcgccatcg gctctgtcga gggctcgggc ggcttcccgc
     3121 atgtgcgatt ccgcgcgttc gatgagttcc acggtggaaa gagggattgg ttcttgtccc
     3181 gatcgcgcgc gtttcaacac atctttggcg cggtcggcca actcccgctg ccgctcggac
     3241 tcggtcttca atcgttgttt cccttgttct gatgcggatt tccgcatggc gtcgagttgc
     3301 tcgcgcaccc agcgttgttc acgttcgagg gtttctcgcg cccgaaccac ctcccggctc
     3361 agggaagaag gttgaccaaa tagatctcgt tctcgctgac cgaggagcac ggcttgctcg
     3421 gcggctttca aggcattgct tgccgcttgg tccgcttccg atagtgcagt tcgctccaac
     3481 gccgccgcca tacgctcggc ctgctcgcgc agcaccatgg cggccccttc gagcgaagac
     3541 atatcggacg aggaagtagg caagccgcgc accgaatctc tcacgatgcg cgcgtgttcg
     3601 cgggccagat cgtgaagccc ttgcatgtcg atggattgca tcgcctcggc aatggcttgc
     3661 tgcacttcct ccatccgaga agcgtggtcc ttggcaagct tttcgatctc atcgccttct
     3721 gagtcgaacg attccatgtc tccgtcgccc gcggaagcgt catccagccc cggcatgccg
     3781 ccgctttccg taccagaacg ccctcctcct ccggacaacg atgggtgggg agagcgtaat
     3841 cgtgcggcca agtgcaacgc cgc
//
LOCUS       DAOSOR010000554         3861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_23692_length_3861_cov_4.856542, whole genome shotgun sequence.
ACCESSION   DAOSOR010000554 DAOSOR010000000
VERSION     DAOSOR010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23692_length_3861_cov_4.856542"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PK710_21485"
     CDS             <1..656
                     /locus_tag="PK710_21485"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOT12366.1"
                     /translation="HTLGAMGTRQAEGEGNPPPTVDVAARLLGVFRNTYYSFPNEAEY
                     SGSKTKIFDASCKLIAEVPTTFHDMLCVQGSGRLASARTVSFAKRNCDCAAVCPRSGQ
                     KICYEALDPAKYPYGRGALGKAITPFRTIAVDDKVIALGTAVFIPAYVSQPMPDGSVH
                     DGCFRAEDRGLKVVGHSVDVFTGGEADTKAWNQLVPTGQGVEVYVDHPRCKARWPDG"
     gene            751..1086
                     /locus_tag="PK710_21490"
     CDS             751..1086
                     /locus_tag="PK710_21490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12367.1"
                     /translation="MATSRENIPEPFRSQIEQATLEFATAIARAFEQSVVAVSHDLVD
                     PHTPRNAAIEKRIESVLQQKRGGWDIEQLHQAVDIPRTTLLSTLQQMVVQGRIVRRYT
                     EDGNRFFPR"
     gene            1145..1615
                     /locus_tag="PK710_21495"
     CDS             1145..1615
                     /locus_tag="PK710_21495"
                     /inference="COORDINATES: protein motif:HMM:NF014019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HOT12368.1"
                     /translation="MRVSTFPLRIRAVRGLLLAAALGTSACGVHQAGIRTGVNPAERL
                     PPVDPAVVQFFGGDDPGPQGQEIGYVISLCDADEKDLALSRLRDAAAKLGADAIVGLR
                     IEIHESTNVVASGRAVRSTSMAQRLAPGSSTTPTISTPAIPTVAPTSPSEEATP"
     gene            1612..2139
                     /locus_tag="PK710_21500"
     CDS             1612..2139
                     /locus_tag="PK710_21500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12369.1"
                     /translation="MKPTIFGSLLLAFSLIGCGQSGFIALAEPASAASPSQYIPIHAS
                     RDIGRPYRVLGFVFRTTEGHKSGLERIDDTMYIMSHDSTEIQELRREALARGADAIVG
                     FEMSPTFDGFGQATGFSVSGYAVKYTATLEPATEAVSTTPTMTSAVPTETPTETPTEP
                     STGTPEPALDSLPTL"
     gene            complement(2358..2954)
                     /locus_tag="PK710_21505"
     CDS             complement(2358..2954)
                     /locus_tag="PK710_21505"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOT12370.1"
                     /translation="MARWAAIVGARGTGKSTHANQVVHLLKRHGICVGGFFQQATEDE
                     LGRRSYDLHRLSTGEVLALARPTTGQELPGHTTYCSFSFVDAAFVQARQWVEQELGSH
                     PVIVIDEVSKVEVSGQGHHDAIRCALGASDDTVVVLCIRADQLFYVVEKFELEDEAIG
                     LLQLPASDEDIVAFVTAIAPPNAIDQQHCSIEPYSSAL"
     gene            3114..3455
                     /locus_tag="PK710_21510"
     CDS             3114..3455
                     /locus_tag="PK710_21510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12371.1"
                     /translation="MIVRVLLFTVPIAWLAGCSSNDNPLLTDVAKQYGQAYCKKLEVC
                     LGVEQFEQSYPGGQDDCVMRTFRIHGTDERSLCTQEQWDRCTKDLEITSCHASDAGVS
                     RPKIPDSCQGC"
     gene            complement(3475..>3861)
                     /locus_tag="PK710_21515"
     CDS             complement(3475..>3861)
                     /locus_tag="PK710_21515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12372.1"
                     /translation="TNIGYLQDGEMQTPLKQEGGEIAWDFAKVIMIPFFLGGQVGSGR
                     FVSDDEWRGVMFGLNWHPTYTAAKPDSLDSIRSFNYLGVQAHVDMGSIRANQPLEPNF
                     QVTVTFLPQIDRDAMFASIGFGSVWY"
BASE COUNT          867 a         1119 c         1054 g          821 t
ORIGIN      
        1 cgcataccct tggagccatg ggaacgagac aagccgaagg ggaaggcaat ccgccgccga
       61 cggtagatgt tgcggcgcgc ctgcttgggg tatttcgcaa tacctattat tcatttccga
      121 acgaagccga atacagtggc tccaagacga agatattcga tgccagttgc aagctcatcg
      181 ccgaagtgcc gaccacgttt cacgacatgc tttgtgtgca gggaagcgga aggctcgcca
      241 gtgcgcgaac ggtgagtttt gctaaacgaa actgcgattg cgcggctgta tgtcctcggt
      301 ccggccaaaa aatctgctat gaggcgctcg atccagcgaa atacccttat ggacggggtg
      361 ctctgggcaa ggctatcacg ccttttcgca ccatcgccgt cgacgacaaa gtcattgctc
      421 ttggaaccgc cgtgttcatt ccggcctatg tatcccaacc catgcccgac ggatccgttc
      481 acgacgggtg ttttcgggcc gaagaccgtg ggctcaaagt cgtgggccac agtgtcgacg
      541 tcttcacggg aggagaagcc gataccaaag cttggaatca actcgttccc acgggacaag
      601 gcgtggaagt gtatgtggat cacccacgct gcaaagcccg ttggcccgac ggataggcgg
      661 gtgggcaacg gaatcccgaa cgcctttcac gcgcggcttg ggatggatgg tagttgattc
      721 ccatcccttt tccgacctaa aagcctcgcc atggccactt cgcgcgagaa catccctgaa
      781 ccgtttcgct cccagatcga acaagcgact ctcgagtttg ccacagcgat cgcacgagca
      841 ttcgaacaga gcgttgtggc ggtatcccac gatcttgtcg atcctcacac ccctcgaaac
      901 gccgccatcg aaaaacggat tgagtctgtc ctccagcaaa aacggggagg atgggatatc
      961 gaacaactcc atcaagccgt cgacattccc cgaacgacac tgctttccac ccttcagcaa
     1021 atggtcgttc aaggacgcat cgtccgtcgt tataccgaag atgggaatcg gtttttcccg
     1081 cggtagctcg atagtgtaac aggtaattta tcgtccttcg cgcatgcgtg ataggaattc
     1141 atgcatgcgt gtatcgacat ttccacttcg aattcgagcg gttcgaggct tgttattggc
     1201 cgcggctctt ggcacttccg cttgtggcgt gcatcaggca ggaatccgaa cgggtgtcaa
     1261 tccggccgag aggttgcccc cggtcgaccc cgcagtcgtc caattcttcg gtggcgatga
     1321 ccctggcccc caaggacagg aaattggcta tgtcatttcc ctatgtgatg cggacgaaaa
     1381 ggatttggca ttgtctcgct tgcgagatgc agccgccaaa ttgggggcgg atgccatcgt
     1441 gggtctgcgc atcgagatcc atgaatcaac caacgttgtg gctagcggaa gagcggttcg
     1501 tagtacgtcc atggcccaaa ggcttgcgcc cggttcgagc acaacaccaa ctatcagcac
     1561 gccagcaatt cccaccgtag ccccgacttc gcccagcgag gaggcaacac catgaaacct
     1621 actatcttcg gttctcttct cctcgcgttt tccctgatcg gttgcggaca atccggtttc
     1681 attgccttgg ccgaaccagc aagcgccgcg tctccttcgc aatacattcc catccacgca
     1741 tcccgcgaca ttggtcgccc ctatcgagtg ctcggcttcg tgttcagaac caccgaaggc
     1801 cacaaatccg ggctcgaacg catcgatgac accatgtaca tcatgagcca tgactccacc
     1861 gagattcagg aattgcgcag agaagccttg gctcgaggcg cggacgcgat cgttggattc
     1921 gagatgagtc ccacgttcga cggcttcggg caggctaccg ggttttccgt cagtggctac
     1981 gcggtgaagt ataccgccac cctcgaacca gcgaccgaag ctgtatccac aacgccaacc
     2041 atgacctcag ccgtgccgac ggaaacgccg acggaaacgc cgacggaacc atcgacgggc
     2101 acaccggaac cggctttgga ttctctcccc actctctgat cttgatccca acaaggatca
     2161 tcgaatcctt tccattcgcg ggttggttct tcccgttggt cacgagccaa gcgcgtacga
     2221 catgctttga catcaaggtc attggcgttg gcgcaagatc ggaaagcagg tcccacgcct
     2281 cgagtagccc gaacagcaca gctcgccccg tggcgaccac atcgacataa ctagctgtat
     2341 ttatatatta ttttcaatca cagggcggat gagtagggct cgattgagca atgctgctgg
     2401 tcgatcgcat tcggtggtgc aatggcggtg acgaacgcga cgatatcctc atcggaagcc
     2461 ggtagttgga gcaaaccgat ggcctcgtct tccagttcga atttttccac gacatagaaa
     2521 agctgatccg cgcgaatgca caatacgacc accgtatcgt cgcttgctcc aagagcgcaa
     2581 cggatggcgt cgtgatgtcc ctgtccggat acttccacct tactgacttc atcgatcacg
     2641 atcacgggat gggatccgag ttcttgctcg acccattggc gagcctgaac gaacgccgca
     2701 tcgacgaaag aaaaggaaca gtacgtggta tgtcctggaa gttcttgtcc ggtcgtagga
     2761 cgggccaggg caagcacctc acccgtggac agtcgatgaa ggtcgtagga gcggcgaccc
     2821 aactcatctt ccgtggcttg ttggaagaat cctcccacgc aaataccgtg gcgtttgagt
     2881 aggtgaacga cttgatttgc gtgggtggac ttgccagtcc cgcgggctcc cacaatggct
     2941 gcccaacgtg ccatgaatac ctcgatttct ggttggcctg gttggctggg gtggtctacc
     3001 ggatatcgtc ccccgtggcc accttgtcga gcccatcgaa gcacgaacga cggatacgtt
     3061 tcgtgcgcgg gctcgattgc catccgcccg tagcctcgct acagtcagtc cccatgatcg
     3121 tccgcgtcct tttattcacg gttccgatag catggctcgc gggctgtagc agcaacgaca
     3181 acccgctgct cacggacgtc gccaaacaat atggccaggc ctattgcaaa aaactcgaag
     3241 tgtgtttggg ggtagaacaa ttcgagcaat cataccctgg agggcaagac gattgcgtga
     3301 tgcgcacgtt tcgcatccac ggcactgacg agcggagtct atgcacccag gagcagtggg
     3361 atcgatgtac gaaggacttg gaaatcacaa gttgtcatgc ctcggacgcc ggcgtgtccc
     3421 gaccgaagat cccagatagc tgtcaggggt gttgaagcgt caaacgcgta aggatcaata
     3481 ccaaacggaa ccgaatccaa tcgatgcgaa catcgcatcg cggtcgattt gaggcaggaa
     3541 ggttaccgtc acctgaaaat tgggttcgag cggctggttg gcgcgaatcg aacccatatc
     3601 gacgtgggcc tgcaccccca agtaattgaa actccgaatc gaatcgaggc tgtcgggctt
     3661 ggccgccgtg taggtgggat gccagttcaa accgaacatg acgcctcgcc attcgtcgtc
     3721 ggatacgaag cggccagacc ccacctgtcc tcccaagaaa aacggaatca tgatgacttt
     3781 ggcaaagtcc caagcgattt ccccaccttc ctgcttgagg ggagtctgca tctccccatc
     3841 ttgtaagtat ccgatattag t
//
LOCUS       DAOSOR010000555         3843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_23817_length_3843_cov_4.223073, whole genome shotgun sequence.
ACCESSION   DAOSOR010000555 DAOSOR010000000
VERSION     DAOSOR010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23817_length_3843_cov_4.223073"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PK710_21520"
     CDS             complement(<1..342)
                     /locus_tag="PK710_21520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12373.1"
                     /translation="MAKLDLTEAEFAELVQEVIRRLARDPGARHLWQWRSRPSCADVP
                     PGPSRRNHLNALLGMLGAATWTVVAGCGSSDCADDPCGGGGNPGQGGTGGSGGGTGGS
                     GGGTGGSGGGTG"
     gene            complement(357..1559)
                     /locus_tag="PK710_21525"
     CDS             complement(357..1559)
                     /locus_tag="PK710_21525"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT12374.1"
                     /translation="MSHCIRHLDLFVTEACNLACPYCFARDRSDAARMTLERARKAIH
                     WLAGSRHSHVHVTFWGGEPLLRLPWLKQVAQELQDRMRDAGKTVSMSLPTNATLLAPD
                     ALDWIERFKVKVFLSIDGDERTQAQRPLASGKNSHELATAGLQAMLHRFGTVTVRMTV
                     TPANVEDLPENIAYFARQGVRRLMVYPAYDQDWSVQDIEAWGEAEKQVAHRLASWIVR
                     MSPRLPIRLDAWMGPLRWMAGLRESRRPTDPISPCGVGESLVALHADGRYSPCHRFTF
                     YGRSKGEPWDLGDDDRGILACDDFRTLTLERQRGQVRCVHCESFAACTMGCVAINYAT
                     TGSLYQVPAWACALQKKRIQACQFLHESLDGHPAYAAYLGQTATALLQTRAQEMGNRA
                     AKLWATLS"
     gene            complement(1556..1759)
                     /locus_tag="PK710_21530"
     CDS             complement(1556..1759)
                     /locus_tag="PK710_21530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12375.1"
                     /translation="MALTAEDREYVIQAAVEILRHPNRDRLLRIVVDRLEADRYQNEH
                     ERELDRVVYALSRTIHDDLSTEP"
     gene            complement(1931..2809)
                     /locus_tag="PK710_21535"
     CDS             complement(1931..2809)
                     /locus_tag="PK710_21535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12376.1"
                     /translation="MKRWLVIGLAVAMGCHSPSKNQSDRGGAKPQPRAIPAPTTLSDT
                     LVSEGAFHNLGFTLAFRIMGDAKEAGKSYAEAMQACRSRGRMLCTESQWLRACEEHSV
                     LGSMESWTATRRGKDVVVAGGKGCGSKEKVKEDDVHPHRMGLCCERAISLRAADAGPW
                     LGLGARLPLRFEQALNQADDKALRGLLADPVVREGRKWSLDDLLAHENKERAQVAWTL
                     FDTCDLRLGPVVVDVSDAGAERKQGMLLTCKTLLAKNGEVIDFTTILGLVGEQEDSLR
                     LVQIDHKGSAYIPGAR"
     gene            complement(2806..>3843)
                     /locus_tag="PK710_21540"
     CDS             complement(2806..>3843)
                     /locus_tag="PK710_21540"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HOT12377.1"
                     /translation="NGLRATGQDYAGVAAMDGSKKSARESSRLLDGAVFEFDFGDRGE
                     NALGSCVCTQRVFMNQVLEDLLSVLQPRQVEDDHFIGESRDLGFMQVFGGQVLGQALN
                     AACRTVDQGWLPHSLHAYFLRPGASDQPILFEVDRIRDGRSFVTRRVVARQRRKAIFN
                     LAASFHVPERGFEHQHRLPDVPGPEGIRSESERMRDIMDRLPERLKQAWLRQRPVEIR
                     VVDPPSMLYPEPRDPIRHAWMRVSGPLPEDPVLHHCLLAYASDFQLLGTCLQPHGKTF
                     MSQDMKVASLDHALWFHRPFRFDDWLLYAMDSPSAAGGRGFNRGSFFDNRGTLVASVA
                     QEALIRRREDT"
BASE COUNT          867 a         1259 c          947 g          770 t
ORIGIN      
        1 gcccgtgcct cctccgcttc cgcccgtgcc tcctccgctt ccgcccgtgc ctcctccgct
       61 tccgcccgtg cctccttgcc ctggattccc tcctcctcca caaggatcat cggcgcaatc
      121 agagctgcca caaccagcca caaccgtcca tgtagcggcc cccaacatgc caagcagcgc
      181 gttaagatgg ttgcgacggc tcgggcccgg tggaacgtca gcacaacttg ggcgagaacg
      241 ccactgccaa aggtgccgtg cgccagggtc ccgagcgagg cgacgaatca cctcctgcac
      301 caattcggcg aattccgctt ccgtaagatc cagttttgcc atggctcatt ccttcgtcac
      361 gacagagttg cccatagttt cgcagctcga ttccccattt cctgcgcacg cgtttgcagg
      421 agtgccgtag cggtctgacc caagtatgcc gcataggcgg ggtggccatc cagcgactca
      481 tgcaagaatt gacacgcctg aatccgcttt ttttgaaggg cgcaggccca cgctggcacc
      541 tgataaagac ttcccgtggt cgcgtagttg atggcgacac atcccatcgt gcatgccgca
      601 aagctctcac aatgcacgca acggacctgc ccccgttgcc gctccaacgt cagcgttcga
      661 aaatcatcgc acgccagtat tcctcgatcg tcatccccca agtcccacgg ctcccctttt
      721 gatcgaccat aaaatgtgaa ccgatgacag ggggaatacc gaccatcggc atgcaatgcc
      781 acgagagatt cccccacacc acaaggagat atgggatccg tgggacgacg cgactcgcgc
      841 aatcccgcca tccaacgcaa aggccccatc cacgcatcga gacgaatcgg gagtcgtggc
      901 gacatgcgca caatccacga tgcaagacgg tgggcaactt gcttttccgc ttcgccccac
      961 gcttcgatat cctgcaccga ccaatcttga tcgtacgccg gataaaccat gagccgccga
     1021 accccttgcc gcgcaaaata ggcgatattt tctggcaaat cctcaacgtt ggccggtgtc
     1081 accgtcatgc gaacggtgac agtaccaaat cgatgcaaca tggcctgcaa gcccgcggta
     1141 gcaagctcat gggaattctt accagaagcg agaggacgct gagcttgtgt tctttcgtca
     1201 ccatcgattg ataaaaacac tttgaccttg aagcgttcga tccaatcgag tgcatccgga
     1261 gcaagaagcg ttgcgttggt gggaagagac atggatacgg tttttcccgc atcacgcatg
     1321 cgatcctgta actcctgggc tacctgtttc agccaaggca acctgagaag cggttctcct
     1381 ccccagaacg taacatgcac gtgtgagtgg cgggatcctg caagccaatg gatggctttt
     1441 cgtgctcgct ccaaggtcat ccgcgctgca tcgcttcgat ctcgagcaaa acaatacgga
     1501 caagcgaggt tgcacgcctc cgttacaaaa agatcgagat gccgaatgca atggctcatg
     1561 gctccgtgct tagatcgtcg tgaatggttc gagacaaggc gtacacgacg cgatccagct
     1621 cccgctcatg ctcgttttga taacgatcgg cctcgagccg atcgacgacg atacgaagga
     1681 gtctatcccg attcggatga cgcaggatct ccaccgccgc ttggattacg tattctcgat
     1741 cctcagccgt caatgccatg gcgatgctct cccaagtcga ccatgaaggg accacaaccg
     1801 caaaaaaacg tcatgggcag tgtaccgtag ccccatgccg gaggccaccg cccttgcctg
     1861 caggctcgct gtgggagagc gaacacccat gcgtgcgatt ccaagacgcc cgaacgggta
     1921 tcccatccat ctatcgcgcc ccagggatgt aggcggaacc cttgtgatca atctgcacca
     1981 atcgcaagct atcctcttgc tctcccacca ggcctaaaat agtagtaaaa tcaataactt
     2041 caccattttt ggccaaaagg gttttgcacg tgagcagcat cccctgcttt cgctcggctc
     2101 ccgcatccga cacatccacc acgaccggtc ccaaccgcag gtcacaggtg tcgaaaagag
     2161 tccaggccac ctgcgcgcgc tccttgtttt cgtgggccaa caggtcatcc aacgaccact
     2221 tccttccttc tcgcaccacc ggatcggcaa gcaaaccacg caacgccttg tcatccgcct
     2281 ggttcaaagc ctgctcgaat cgcagaggca atcgcgctcc caaccccaac cacggtcctg
     2341 catcggctgc ccgcagcgag atggctcgct cacaacacaa ccccatccgg tggggatgca
     2401 catcgtcttc tttcactttt tccttcgaac cgcagccctt tccacctgcc accaccacgt
     2461 ctttgccccg gcgcgtcgcc gtccaggact ccatggaacc caacaccgaa tgctcttcgc
     2521 atgcccgcag ccactgggat tcggtgcata acatgcgccc ccggcttcga caggcttgca
     2581 tggcttccgc gtaactcttc cccgcctctt tcgcatctcc catgatccga aaagcaaggg
     2641 tgaaccccag gttgtgaaaa gcaccttccg aaacaagcgt atccgataac gtcgtagggg
     2701 ctggaatcgc gcggggttgg ggcttggcgc cccctcgatc cgattgattt ttcgagggag
     2761 aatggcatcc catcgccacg gcaagaccaa tgaccaacca acgtttcatg tatcctcacg
     2821 acgacgaatg agtgcttctt gcgccacgga agccaccaga gtcccgcgat tatcgaagaa
     2881 gctgcctcgg ttgaatcctc tgccccctgc cgcactgggg ctatccatcg catataatag
     2941 ccaatcatcg aaacgaaagg gacgatgaaa ccacaacgca tgatcgaggc tcgccacttt
     3001 catgtcttgt gacataaacg ttttgccatg gggttgaaga caggtgccaa ggagctgaaa
     3061 atctgatgca taggcgagca aacagtgatg caggaccgga tcttccggca atgggcccga
     3121 gactctcatc catgcatgcc gaattggatc ccggggctcc gggtagagca tcgatggtgg
     3181 atcgacgacc cgaatctcca ccggccgctg ccgcaaccac gcctgtttca gccgttccgg
     3241 aagccgatcc atgatatcgc gcattcgttc cgattccgaa cggattccct cgggccccgg
     3301 gacgtccggc aggcgatgct gatgctcgaa ccctctttct gggacatgaa acgacgcggc
     3361 aagattgaaa atagcctttc ttcgttggcg cgccaccacc cggcgcgtta cgaaactgcg
     3421 cccatcacgg atgcggtcga cttcgaaaag aatcggctga tccgacgctc ccggacgtaa
     3481 aaaataggcg tgcagcgaat gcggcaacca gccttggtcc accgtccgac acgcggcatt
     3541 aagcgcctga ccgaggactt gccccccaaa tacctgcatg aacccaaggt cacgactctc
     3601 cccgataaag tgatcgtcct ccacttgcct gggttgaagc accgataaca agtcttcgag
     3661 tacctgattc atgaaaaccc tctgcgtaca cacacaagac cctaaggcat tctcgcctct
     3721 atcaccaaag tcaaattcaa aaacggcacc gtccagcaga cggctcgatt ccctagctga
     3781 tttcttgctg ccatccatcg ccgccacgcc cgcatagtct tggcccgttg cgcgcaaacc
     3841 gtt
//
LOCUS       DAOSOR010000556         3827 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_23933_length_3827_cov_3.655620, whole genome shotgun sequence.
ACCESSION   DAOSOR010000556 DAOSOR010000000
VERSION     DAOSOR010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23933_length_3827_cov_3.655620"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PK710_21545"
     CDS             <1..195
                     /locus_tag="PK710_21545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12378.1"
                     /translation="LGFLMGLCPVLAMQAWVPKLGLSLVTPAWLVASVFGGAVLLCLA
                     SATASFRTIATVDPALVFRG"
     gene            complement(324..2042)
                     /locus_tag="PK710_21550"
     CDS             complement(324..2042)
                     /locus_tag="PK710_21550"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HOT12379.1"
                     /translation="MNIGENTGKIEGKLPGVAEGKCGDGLDSDGDGFVDDGCPCTPGQ
                     IAPCFPGPIEARHQGECADGLMLCPGRGEFSFWVGDCSGAVTPTEELCDGVDNDCDGA
                     VDEGCDCGTVGESRSCGWSPNLSLPCKPGQQTCQSNGTWSDCVGVVFPAEETCGDGID
                     NDCNGLVDDGCGEPDGGEDAGVDTGTVEDDGGLTQEICGNGLDDDRNGFIDDGCEVVA
                     YCPPDSVRWDRIQSPDGLTLGSWEAARSAWTGKEHLFAYVGKEEPYGQGQNYKLYLAR
                     WGSDGSHLSTTMIADKNVKNPFSRTDDLVWTGDLAVILASGMLFRANADGLSVGVPVA
                     TNLGRAIGWNGKRIGMLSIRSGTQAFFEELNTQFEPVASVELDGLGWSDVAWTGTHWI
                     VMGTSSKKVVFLDGTTVVATHALPTEQYTWPFMAVSNHGALVCGHTFRSDFSDPYSAR
                     CQLFSGNGELRGDPIAWDGGPFRHGDSGAFWWKSRYMVIFIGDDVGKNTVRIVTIGDD
                     GSVQDVPTPPLGADTGHTPGSWTWYFHSAFDNIEYTPDEIFFETRWLSPYMKSGRMRV
                     YCDTTQ"
     gene            complement(2039..2176)
                     /locus_tag="PK710_21555"
     CDS             complement(2039..2176)
                     /locus_tag="PK710_21555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12380.1"
                     /translation="MKSLERRDNPRTIPAVDMDFGQKDLQGQLGLSFLPSWLPQHARA
                     P"
     gene            complement(2173..2973)
                     /locus_tag="PK710_21560"
     CDS             complement(2173..2973)
                     /locus_tag="PK710_21560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12381.1"
                     /translation="MQDESFDSRDDASLPVPVQVGLAAMRARGPSPEAVERLRAGLGL
                     AGPSGPEQPPEPSPPHPASSPVEPPPAPTILSLPILKSASAVLLAAVVGGLLWFATRP
                     PSPAAVFPSGSSATSAPSLPTLAASSSPQIVQPPVSSASVLDEPKIVRPAESKPASAV
                     PSELDLIQQAQRALHSHPQRTLSLCREHARRYPHGMLAQEREVLMVEALQRLGHDEEA
                     KDRSRAFLKDNPDSAHRKKIEHVTGQPSSSSSAPAHRPSDAPKSKRHE"
     gene            complement(2963..3514)
                     /locus_tag="PK710_21565"
     CDS             complement(2963..3514)
                     /locus_tag="PK710_21565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT12382.1"
                     /translation="MALADMDESSGPRLEFSQVFESHVSFVWRVLEHHGIRGDDVEDA
                     AQEVFIIVAKKLATLNDPSCLRSWLFGIARRVAASHRRRAHVRREQLASDPGKDSADM
                     GQHARNEQVEALTRLESVLGELKEEQRMAFILREVEQLSIQEVAQALGCPLQTAYSRV
                     KAAREHVIAAFREPNPQEVVDAG"
     gene            complement(3547..>3827)
                     /locus_tag="PK710_21570"
     CDS             complement(3547..>3827)
                     /locus_tag="PK710_21570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12383.1"
                     /translation="SLCAGFQAGRILEAGAGFDHNNDSAAGVGGLWVGVDAQIPGERS
                     WGMRLGLSGGVPLVRHQIEFTNESGDSQTLYQSSGVYGRLALTFVHIP"
BASE COUNT          847 a         1173 c          990 g          817 t
ORIGIN      
        1 ttgggatttc tgatgggtct ttgccccgtg ttggcgatgc aggcgtgggt gccgaaacta
       61 ggtctttcgt tggtcacccc ggcatggctg gtagcctcgg tattcggagg ggctgtattg
      121 ctatgtttgg cctccgctac agcctctttt cgcaccatcg ccaccgtcga tcccgccctg
      181 gtgtttcgcg gctaaaagcg tgttccgaag cgaaccccgt ccatgccgac ggatcgctag
      241 aggcttgttg cgacgacatc ctgtccccat ccggacggcc atcatgatag tccaattaaa
      301 tccatgagtt gaatacaaat aggctattgg gtagtatcac aatagacccg catgcgcccc
      361 gatttcatat agggggacaa ccagcgcgtt tcgaaaaaga tctcgtctgg cgtgtactcg
      421 atattgtcga aggcactatg aaaataccat gtccagctcc ccggcgtgtg acccgtatcg
      481 gcacccaatg ggggcgtcgg cacgtcctgc acagaaccgt cgtcgccgat agtcacgatt
      541 cgaaccgtgt ttttgccgac atcatcgcca ataaaaatga ccatataccg cgatttccac
      601 cagaaagcac cgctgtcccc gtgtcgaaaa ggtcccccat cccatgcaat cggatcacct
      661 cgaagctcac catttccgga aaaaagctga caacgggcgg aatacggatc gctaaagtcg
      721 cttcggaacg tatgaccaca taccaacgcc ccatggttgc tgacggccat aaaaggccat
      781 gtgtactgct cggtgggcaa agcgtgcgtg gccaccaccg tcgttccatc gagaaacacg
      841 acttttttcg aactcgtccc catcacgatc caatgggttc ctgtccacgc gacatcgctc
      901 caaccaagtc catcaagctc cacactcgcc accggctcga actgcgtatt caactcttcg
      961 aagaaagcct gcgtgccaga acgaatggaa agcatgccta tccgtttgcc gttccagcca
     1021 atcgcccgtc ccaggttggt cgccaccgga actcccacgg aaagtccgtc ggcgttggcg
     1081 cgaaatagca taccagaagc caaaatgacc gctagatccc ccgtccacac caaatcatcg
     1141 gttcgggaaa acggattctt cacgttctta tccgcaatca tggtcgtgct caaatggctg
     1201 ccatcgctcc cccaacgcgc aagatataat ttataattct gaccttgacc atacggttct
     1261 tcttttccca cataggcgaa aagatgctcc ttgcccgtcc acgcgctgcg cgcggcttcc
     1321 cacgacccaa gcgtaagccc atccggtgat tggattcgat cccatcgaac ggaatcaggg
     1381 ggacagtaag ccacaacctc acacccatca tcgatgaacc cgttgcggtc gtcgtccaac
     1441 ccattgccac atatctcttg ggtcaaaccc ccatcgtcct ctaccgtccc cgtatccact
     1501 cccgcatctt cccccccatc aggctcacca cacccatcat ccaccaggcc gttgcaatca
     1561 ttgtcaatcc catcgccgca agtctcttcg gcaggaaaga cgacgcctac acaatcggac
     1621 caggtaccat tcgattgaca ggtctgttgt cccggtttgc acggcaaaga aaggttggga
     1681 gaccaaccgc aacttctcga ttctcccacg gtaccacaat cacatccttc gtcgaccgcc
     1741 ccatcgcaat cgttatccac tccgtcacag agttcttcgg tgggcgtcac ggcaccggaa
     1801 caatccccaa cccaaaacga aaactcaccc cgaccggggc aaagcatcag cccatcggcg
     1861 cattcgcctt ggtgtcttgc ctcaatcggc ccaggaaagc agggcgcaat ctggcctggg
     1921 gtacacggac acccatcgtc cacgaatccg tcgccatcgc tgtccaggcc atcgccgcac
     1981 ttgccttctg ccacccccgg gagttttcct tcgatttttc cggtattttc accgatattc
     2041 acggtgctcg tgcatgctgc ggcaaccacg agggcaaaaa ggacagccca agctgccctt
     2101 gcaggtcttt ttgtccgaag tccatgtcca cggccggaat ggttcgtggg ttgtcccggc
     2161 gttcgagact tttcactcat ggcgcttgct cttgggtgca tcggagggtc ggtgagcggg
     2221 agccgacgat gagctgctcg gctgccccgt aacgtgttct attttttttc ggtgcgcgct
     2281 atctggattg tctttcaaaa acgctcgcga acggtctttg gcttcttcgt cgtggccgag
     2341 ccgttgcaaa gcctcgacca tgagcacctc ccgttcttgt gcgagcatgc catgagggta
     2401 acggcgcgcg tgttctcggc acaaactcag ggtacgttga ggatggctat gcagtgcccg
     2461 ctgagcttgt tggataaggt cgagttcgga aggtacggcg gatgctggtt tgctttcggc
     2521 agggcgcacg atcttcggct cgtccagaac ggatgcagaa gaaacggggg gttgcacaat
     2581 ctgtggcgat gaactcgccg ccagggtggg caacgacggc gcgctcgttg cggaagagcc
     2641 agaagggaaa acagccgcgg gcgatggtgg acgagtggca aaccacagca atcccccaac
     2701 cacggcggca agcaacaccg cgctggcgct ttttaggatg ggcaacgaca aaatagtcgg
     2761 tgcgggcggg ggttcgacgg gagaagaagc aggatgggga ggcgacggtt caggaggctg
     2821 ctcagggcca gaaggaccag ccagacccaa acccgctcgc agtctttcga ccgcttcagg
     2881 cgacggaccg cgagcacgca tggcggcgag cccaacctgc acgggaaccg gcaagcttgc
     2941 gtcgtcgcga gaatcaaagg attcatcctg catcgacgac ctcctgaggg ttgggttctc
     3001 gaaaagcggc gatcacatgc tcacgggctg ctttcacacg agaatacgcg gtttgtaacg
     3061 gacatccaag cgcttgcgcc acctcctgaa tcgagagttg ttcgacttca cgcaaaataa
     3121 atgccattcg ctgctcttct ttgagttctc caagcacgga ctcgagcctg gtcaaggctt
     3181 ccacctgttc atttcgggca tgttgaccca tatcggcact gtcttttccg ggatcacttg
     3241 cgagctgttc cctgcgaaca tgcgctcttc gtcgatggga agcagcaacc cgtcgggcaa
     3301 tcccgaacag ccaagagcgc aaacaagaag ggtcattcaa tgtcgcgagt tttttcgcca
     3361 cgatgatgaa tacttcttgg gctgcgtctt ccacatcgtc accgcggatt ccatgatgtt
     3421 cgagcacccg ccaaacgaaa ctcacatggc tttcgaacac ttgcgaaaac tcgagtcgag
     3481 gacccgatga ttcatccata tccgcaagag ccatgctgcc taccgtccga aattctgata
     3541 agtttgttat ggaatgtgta cgaaggtcaa cgccaggcgt ccatacaccc cggagctttg
     3601 gtagagggtc tgagagtccc cggattcatt ggtaaattcg atttggtggc gcaccagcgg
     3661 cactccaccg gatagaccga gtctcatccc ccagcttcgt tcgccaggga tttgggcatc
     3721 gactccaacc caaagaccgc caacccccgc ggcgctatcg ttgttgtggt caaaaccagc
     3781 ccctgcttcc agaatacgtc ctgcttggaa accagcacat agggaag
//
LOCUS       DAOSOR010000557         3824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_23963_length_3824_cov_3.594587, whole genome shotgun sequence.
ACCESSION   DAOSOR010000557 DAOSOR010000000
VERSION     DAOSOR010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23963_length_3824_cov_3.594587"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PK710_21575"
     CDS             <1..333
                     /locus_tag="PK710_21575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HOT12384.1"
                     /translation="SGGSGGSGAEGGYAGEGGSAGGPGGEAGTAGIAGSAGVAGNAGS
                     GPEAGGAGSPGASGKPNDNGPKTNSDDDSDSGCGCSTVGHSSSTASALLLGLAMAFCR
                     RLRRRRAA"
     gene            complement(402..1430)
                     /locus_tag="PK710_21580"
     CDS             complement(402..1430)
                     /locus_tag="PK710_21580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT12385.1"
                     /translation="MATLPFVTLGIPCLNEEDFIEPLVRGALAQDYPSDRFEVLVADG
                     GSTDRTLEILERLSKEDCRLRVLHNPGKVQAAGMNEIIKAARGDVLVRFDAHADYAQD
                     YVRKCIEVLGRTGADNVGGGAKTRSKNWFQRAVCAALDSPLGVGGSRYRTEGQEGYVD
                     TVWGGTFPRRIFETVGMYDPKAVVNEDAELNQRILEAGGKIYLSKEIIAYYYPRDSFR
                     GLAKQYFKYGKGRARTLVKHRTFPTPRPAIPFLMVATGALLLVVPPMQALAPLYFSSY
                     GLLTAVEAVRVGRKAGVAAIPAIWAIFPVLHVAHGVGFGTGLIHFLRHPDWAEPERLA
                     PRTTGAGE"
     gene            1550..2509
                     /locus_tag="PK710_21585"
     CDS             1550..2509
                     /locus_tag="PK710_21585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12386.1"
                     /translation="MKKKLAIAAVAVVAVLGTLLIPVGGKAHSIPDNKDKSMDLSSYP
                     ADKPSTPWRLLFIHHSVGGQLLADPGADQELAPSIHATHPNGGGLRKALEGQGYIVRE
                     ASYESQVGDKTDLFDWLPKFRHDMDKVLRVSFNDQTTAENEKNRIVLFKSCYPNSRFE
                     GLGQPPGNPDGPALTVWNAKASFNALLDEFRKHPDTLFVYFTAPPNAPVYGAEPLFKV
                     LLRKVMRKPTTDEAMASQASFARSFNNWVKSPEGWLKDYPLHNVVVFDYFDLLTNEGA
                     SNFLQYPTGNGDDSHPSRAGQQKAAAAFIPFLNRAVHRAALTP"
     gene            2603..3670
                     /locus_tag="PK710_21590"
     CDS             2603..3670
                     /locus_tag="PK710_21590"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOT12387.1"
                     /translation="MSTTRLSVAARTDAGRVRDNNEDSFTVADLNTGERIDDPSAMDG
                     ILVKDRGVLLVVSDGMGGHAAGEVASALVVDSLRGTLGEMDDHSESAHQRLEEAVKRV
                     NANVYEAARSSAREGMGATLTAVVVHQREAYIAEVGDSRAYVLRRGRLRQMTKDQSFV
                     QMLIDSGAITTDQAKNFFQKNWIVQAMGLEANVHVALGRLQLRTGDRLLICSDGLYSL
                     VEEHELAELLAGDDLTQACHQMIDLANERGGTDNVTAIVALIESQDLPFAGHDESVTQ
                     TFEVLKEYAGEKIVDPSRSDSRAASTLPPSDSQDSKPQSQIQTVRPPAPDNGKSSFFI
                     IILGILALIVVALLGYWFFSS"
BASE COUNT          858 a         1336 c          905 g          725 t
ORIGIN      
        1 tcaggcggct cgggtggctc aggcgcggaa ggaggctacg ccggcgaagg tggctccgca
       61 ggcggcccag ggggcgaagc tggtaccgcg ggcatcgctg gctcggcagg tgtcgctggc
      121 aacgcaggat caggccccga agccggcggc gcgggctccc caggtgccag cggcaaaccc
      181 aacgacaatg gccccaaaac caacagcgat gacgacagcg acagcggctg cggttgctcc
      241 accgtcggtc actcctcctc cacggcctcc gcccttctgc tcggcctcgc catggctttt
      301 tgccgtcggc ttcgtcgacg ccgcgcagcc tgatccctcg gtgagcggca cgcggccctt
      361 cgcgcccgct atgtaacaac taattgtatt tattgattaa tttactcgcc cgctcctgtc
      421 gtccgcggcg caagccgctc gggctccgcc caatccggat ggcgcaaaaa atgaatgagt
      481 cccgtgccaa accccacccc gtgggctacg tgaaggactg gaaaaatggc ccatatggca
      541 ggaatggcgg ccacgccagc ctttcgcccc acacgaaccg cttccaccgc cgtcagcaaa
      601 ccataggagc tgaaatatag cggtgccaaa gcctgcatcg gcggcaccac gagcaacaac
      661 gcgcccgtcg ccaccatgag aaaaggaatc gcgggccggg gcgtgggaaa agtccggtgt
      721 ttgacaagcg tccgcgctcg tcccttgccg tacttgaaat actgcttcgc cagcccccga
      781 aacgaatccc tggggtaata atacgcaatg atctccttgc tcaaataaat cttcccccca
      841 gcctccaaaa tgcgttggtt caactccgca tcctcattga ccaccgcctt gggatcatac
      901 atccccacgg tttcgaaaat tcgcctcgga aacgtccccc cccataccgt atccacatat
      961 ccctcctgcc cctccgttcg gtatcgcgac ccccctaccc ccaacggact atccaacgcc
     1021 gcgcataccg ctcgctgaaa ccagtttttc gaacgggttt tcgccccacc ccccacatta
     1081 tccgcccccg tgcggcccaa cacttcgatg cactttcgca cataatcctg cgcataatcc
     1141 gcatgcgcat cgaaccgcac gagcacatca ccccttgcgg ccttgatgat ctcattcatc
     1201 ccggccgctt gcactttgcc cgggttgtga agaacccgta aacgacaatc ctccttcgat
     1261 aatcgctcga gaatctccag cgtacgatcc gtgcttcccc catccgcgac caacacttcg
     1321 aacctgtccg aaggataatc ctgagcaagc gcgcctcgca ccaaaggctc aatgaagtcc
     1381 tcttcgttca aacacggtat gcccaacgtc acgaacggaa gtgtcgccat cgggcgcaca
     1441 ttatcacgtt gcaccgtgtg agtaaatgta tccaatctcc aattgacgat atgcctgtcc
     1501 gtgttaagtt ccatcgtcgt cgcttgccgc acggaggaac ctaaccccca tgaaaaaaaa
     1561 actggctatc gccgctgtgg ctgtcgtggc cgtgctggga accctgctga ttcccgtggg
     1621 cggcaaagcc cattcgattc cagacaacaa ggataaaagt atggacctat cgtcctatcc
     1681 cgccgacaaa ccctccaccc cgtggcgtct actattcatt caccattccg tgggaggaca
     1741 attgctggca gaccccggcg ccgaccagga actcgccccc agtatccacg ctacccaccc
     1801 caacggcggc ggattgcgca aagccctcga aggacaggga tatatcgtcc gagaagcctc
     1861 ctacgaaagc caagtaggcg ataaaactga tctttttgac tggctcccca agtttcgcca
     1921 cgatatggac aaggtcctgc gcgtctcttt caacgaccaa accaccgccg aaaacgaaaa
     1981 aaaccggatc gtgctgttca agtcctgcta cccgaacagc cgattcgaag gcctgggaca
     2041 accccccgga aacccggacg gaccagcctt gaccgtctgg aacgccaaag cctccttcaa
     2101 tgccctgctc gacgaattcc gaaagcatcc cgacacgctt ttcgtgtact tcaccgctcc
     2161 ccccaacgct ccggtttacg gtgccgagcc gcttttcaaa gtcctgctcc gaaaagtcat
     2221 gagaaaaccc acgaccgatg aagccatggc ctcacaagct tccttcgctc gttccttcaa
     2281 taattgggtc aaatccccgg aaggctggct caaagactat cccctgcaca acgtcgtggt
     2341 cttcgattac ttcgaccttt tgaccaacga aggcgcatca aatttcctac aataccctac
     2401 gggaaacggc gatgacagcc atccttcccg cgccggacaa caaaaagccg ccgccgcttt
     2461 catccccttc ttgaaccgcg ccgtccatcg agccgcactg accccctgaa cctcctgttc
     2521 gaacaaagcc ttgcacctac gccccttttc ggtacaaggt tgccctgccc atgaattcga
     2581 cgcttccgac acgaggacag cgatgtcgac cactcggctg agcgtagctg cacgtaccga
     2641 cgctggaaga gttcgcgata acaacgagga ctcttttaca gtcgccgact tgaataccgg
     2701 tgaacgaatc gacgatccct cggccatgga cggcatccta gtcaaggacc ggggcgtgct
     2761 tctcgtcgtc tccgacggca tgggcggaca cgccgccggc gaagtggcca gcgctctggt
     2821 cgtcgattcg ctccgcggca ccctcggcga aatggacgat cactccgagt ccgctcacca
     2881 acgcctcgaa gaagccgtca aacgcgtcaa cgccaacgtg tatgaagcgg ctcgatcgag
     2941 cgccagagaa ggcatgggcg ccaccctcac cgccgtggtc gttcatcagc gcgaagccta
     3001 tatcgccgag gtcggagact cccgcgccta cgtgcttcgc cgcggtcgtt tgcgtcaaat
     3061 gaccaaagac cagagcttcg tccagatgct catcgactcg ggagccatca ccaccgacca
     3121 ggccaaaaac tttttccaaa aaaactggat cgtccaggcc atgggcctgg aagccaatgt
     3181 tcacgtcgcc ctcggacgac tacaattgcg caccggcgat cgattgttga tttgctccga
     3241 tggcctctac tcgctggtgg aagaacacga actcgccgaa ttgctggccg gcgatgacct
     3301 cacacaagcc tgccaccaga tgatcgatct cgccaacgaa cgtggaggaa ccgacaacgt
     3361 taccgccatc gtcgccttga tcgaaagtca agacctgccc ttcgcgggac acgacgaatc
     3421 cgtcacccaa acgttcgaag tcctcaaaga atatgccggt gaaaaaatcg tcgacccttc
     3481 ccgtagcgac tctcgcgcgg cttccactct ccccccatcc gacagccagg attccaaacc
     3541 ccaatctcaa attcaaaccg tccggcctcc cgcccccgac aacggtaaat cctcgttttt
     3601 tataattatt ttaggaatcc tcgccctgat cgtcgtggcc ttgctcggct actggttctt
     3661 cagctcctga agcgccaaac gccccaacag cccctgcact tctcgcacat atcctctcaa
     3721 caccccccga tccaactccg taaaacgctc cctcatatcc gacggcacca cccccaacgc
     3781 cacactgcgc tgccgaagcc cctcgatgag cctttgccca tcca
//
LOCUS       DAOSOR010000558         3822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_23981_length_3822_cov_3.947704, whole genome shotgun sequence.
ACCESSION   DAOSOR010000558 DAOSOR010000000
VERSION     DAOSOR010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23981_length_3822_cov_3.947704"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PK710_21595"
     CDS             <1..575
                     /locus_tag="PK710_21595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT12388.1"
                     /translation="EPPSAPLTLLSEQGPIPAANAQRILHIGDSMVPLVGNYLRSVFT
                     QSKRRYEIISIPSSSTLSWAQEHGLRDAVYRYDPQVILISLGSNELFDPNPNRRAAAI
                     RQIVSETRGRPCLWVGPPAWKKDKGFLQVLRENMGHCRYFDSTQLKLERMADGRHPSW
                     NGSYHWAEAVWKELGGTEPLPTGQGKPSSP"
     gene            706..1491
                     /locus_tag="PK710_21600"
     CDS             706..1491
                     /locus_tag="PK710_21600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOT12389.1"
                     /translation="MTRQRFGRVALSDGVQAYVRVQEDRLELLRTAPWFGVHEMGREV
                     AIDQATWLCPAQPTKILCIGRNYRAHAKEMGADVPTAPLLFLKPPSSLLAPQGTVLLP
                     PESAQVEHEAELGVIIGRRARRISVDQALDFVFGYVAVGDITARDLQRADGQWSRAKG
                     FDTFCPVGPEIVTGIDPCALRVIGRVNGVVRQDGYTKDMIFNVAQLVSHLSQAMTLEP
                     GDLVATGTPSGVGPLSDGDRFEVVIENIGTLAVSVRNEKTTAP"
     gene            1574..3352
                     /locus_tag="PK710_21605"
     CDS             1574..3352
                     /locus_tag="PK710_21605"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HOT12390.1"
                     /translation="MRESGRTQSSPSIDPRQGLARGSRYAILCSLVMTVVGTAACARD
                     NLSNSNIETSTVTSPLVADQEQKLIGTDTTLFGGSVAISSDYAFVGSIEPKSFSGSVV
                     VYQRSGSSWSKSQTLTEASGDGDLLGASLAVDGDTLVAGASMLENGTLPGRVYVYRRT
                     GTKWSQTQTLQASAGYPFDGFGYVVALSQGVLAVLTTEGEAYAFQDSGGSFTKGTYLD
                     ISDMLATAIDTDGTRIAVGLPSHTGGGAVYVFDKTGSGWNSTMVKASDSVSGDKFGTS
                     VLIRGNTLFVGSPRNTSKAVLGKVYVFKDSSVGWSATATLTASDGIIRDYFGMSLAWM
                     DGALLVGAPYDGSSQAKAGAAYWFFERNGGWQEEGKIAASDGSVRNSFGIAVAMSSTR
                     AIVGAGPNIPLGYNAVGARAAYVFSLQRQVGDPCTQNDQCSSASCVNSVCSGVPEGGT
                     GGAAGAGGSGGAGGSGAAAGSGASAGTSGLGGEGGTAGAGAMGGANNTAGNAGSGEPD
                     GAVGKAGGAATGDQDAGVDTPAHEQFTDTTDVSGFYACSTIDAHGGRHPIGVVLGLGL
                     IALSRRRYRDRSGALRPRSDSRNRFP"
     gene            complement(3428..>3822)
                     /locus_tag="PK710_21610"
     CDS             complement(3428..>3822)
                     /locus_tag="PK710_21610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOT12391.1"
                     /translation="PGELIFREGDTSSEMYVVLHGEFEVLKNSKRGVDTRVALLGTGD
                     WFGEMSILDVQPRSASVRSLAVSRLLVITTADLEALYRRDVKSYSLFVLNVARELSRR
                     LRVTDGILAELAASMVDDVVAKRHGSST"
BASE COUNT          837 a         1063 c         1122 g          800 t
ORIGIN      
        1 ccgaacctcc ctctgccccc ctgaccctat tgtccgaaca aggtccaatt ccagccgcaa
       61 acgcacagcg aatccttcac atcggcgact ccatggtccc actcgtggga aattatttac
      121 ggtccgtttt cacccaatcc aagcgacgtt acgaaatcat cagcatccct tcgtccagta
      181 ccttgtcgtg ggcgcaagag catggtttac gcgacgccgt atatcggtac gatccgcaag
      241 tcatcctcat ttcgcttgga tccaacgagc tattcgatcc caatccgaat cgccgagcag
      301 cggccattcg gcaaatcgtc tccgaaacac gcggacgtcc ctgcctgtgg gtcggacctc
      361 ccgcttggaa aaaagacaaa gggttcttgc aagtactccg tgagaacatg ggtcattgcc
      421 gatacttcga ctcgacccag ttgaagctcg aacgcatggc cgacggtcgc catccaagtt
      481 ggaatggaag ctatcattgg gccgaggccg tgtggaaaga gcttggtgga acggagcctt
      541 tgccgacagg ccaggggaaa ccatcgagcc catgagccgc ctaaattaga tgtaacatat
      601 tggaattatt aatttattca ttaacatgga caggatggat acgagaggat tgggcgacgt
      661 gccaggtgac aggttcgacg aggcttcgat acgatagcca cctgcatgac tcgacaacga
      721 tttggaagag tagccctttc cgatggcgta caagcgtatg ttcgggtgca agaagatagg
      781 ctcgaactac tccgcacggc gccttggttt ggcgttcatg aaatgggtcg ggaggtggcg
      841 atcgatcaag ccacctggtt atgtccggcg cagccgacca agatcttatg catcggcaga
      901 aactaccggg cccatgccaa ggaaatggga gccgacgttc ccaccgctcc attgctcttt
      961 ctcaagccac cgtcttcgct ccttgccccc cagggaaccg ttttgttacc tcccgaaagc
     1021 gcgcaagtgg agcacgaagc cgaactcggc gtgatcatcg gtcgtcgcgc gcgacgtatt
     1081 tccgtcgacc aggcattgga tttcgtgttc ggatacgtag cggtgggcga catcaccgcc
     1141 cgcgatcttc aacgggccga tggccaatgg tctcgcgcca aagggtttga caccttctgt
     1201 ccggtggggc ctgagattgt gacaggcatc gatccgtgcg cgttgcgagt catcggacgc
     1261 gtcaacggcg tcgtgcggca agatggctac accaaggaca tgattttcaa cgtcgcgcaa
     1321 ttggtatcgc atttgagcca ggcgatgacg ctcgagcctg gagatctcgt ggccacgggt
     1381 acgccatcag gagtgggacc gctgtccgat ggggaccgat tcgaggtcgt gattgagaat
     1441 attgggacac ttgccgtatc cgttcgaaac gagaaaacta cggccccttg accgcgtgcc
     1501 gctcgaatat tgtatctcct gattcgaacc gacgtcgaat tctttccctc attagtgcta
     1561 gacatactga accatgcgtg agtcaggtcg aacccaatcc tccccttcca tcgaccctcg
     1621 acaaggttta gctcgcggct cgcgatacgc cattctctgt tcgctggtta tgacggtcgt
     1681 tggcaccgct gcctgcgctc gcgacaatct ctcgaattcc aatatcgaaa cgtccaccgt
     1741 aacaagcccg ctcgtggcgg atcaggaaca gaaactcatc ggcacggaca cgacgttatt
     1801 cggaggaagc gtggccatat catcggacta cgctttcgtg ggttccatcg aacctaaatc
     1861 tttttcggga tccgtcgttg tgtaccaacg cagcggctct tcgtggtcca agtcgcaaac
     1921 gttgaccgag gcttcgggag atggcgattt gttaggagct tccctcgccg tggacggaga
     1981 cacgttggtc gctggggcca gtatgctcga aaacggaaca ctaccgggac gggtatacgt
     2041 ctatcgtcga acgggaacga agtggtccca aactcagact ctccaagcat ccgcaggcta
     2101 tccgtttgat ggattcggct atgttgttgc tctttcacaa ggggtattgg ccgttttgac
     2161 taccgaagga gaagcctatg cgttccaaga ctcaggaggc tccttcacca aaggaaccta
     2221 cttggacatc agcgacatgc tggcaactgc cattgacacc gatggcactc gtatcgccgt
     2281 aggcctgcca tcgcatactg gcgggggcgc tgtttacgtt ttcgacaaga cagggtcggg
     2341 ttggaacagc accatggtca aagcttccga ttcagtttcg ggcgacaagt tcggaacctc
     2401 ggtgctgatt cggggtaaca ctttgttcgt agggagtcct cgtaacacct ccaaagcagt
     2461 tcttgggaaa gtctacgtat tcaaggacag ttctgtcggg tggagtgcga cggccacctt
     2521 gacggcatct gacggcataa ttcgggatta ttttgggatg tcgttggcgt ggatggacgg
     2581 ggcgttgttg gttggtgctc cttacgatgg ctcctcccaa gccaaagccg gcgctgccta
     2641 ttggtttttc gaaagaaacg gcggctggca agaggaggga aaaatcgcgg cctcggatgg
     2701 cagcgtaaga aattcgtttg gaatcgccgt cgcgatgagt tcgacacgcg ctatcgtggg
     2761 cgcgggtccc aacatcccgc tcggctataa tgctgtgggg gccagggctg cgtatgtgtt
     2821 ttcgttgcag cgacaagtcg gagacccctg cacgcaaaac gatcaatgca gttccgcatc
     2881 gtgtgtcaac tccgtgtgta gtggggtacc cgagggtggc acggggggag cggcaggggc
     2941 gggcggctcg gggggagctg gtggttcggg agcagcagcc ggctcggggg cgtcggcagg
     3001 gacaagcggc ttgggagggg aaggaggaac cgccggagca ggagccatgg gaggagccaa
     3061 caacacggcg ggaaatgctg gatctggaga gccggatggt gcggtaggga aagcaggggg
     3121 ggcagcgaca ggagaccagg acgccggagt ggatactccg gcacacgagc agttcacgga
     3181 taccacggat gtctcagggt tctacgcctg ttcgaccatc gacgcccatg gtggccgtca
     3241 tccgatcggt gtcgtcctcg gacttgggct catcgcgctt agtcggcgcc gataccgcga
     3301 ccgatcggga gcccttcgcc ctcgaagcga ttcccgcaac cgctttccat gatcgagtcg
     3361 tccatatcca cggatacgcg gactatccgt atccggatac tatgcaaatc ggtcacatgc
     3421 ggcccgttca ggtggacgag ccatgccgtt tcgccaccac atcatccacc atgctcgccg
     3481 caagctcggc aaggatacca tcggtcacgc gcaaccgtcg gctcagttcg cgcgccacat
     3541 tgagtacgaa caaggagtac gacttcacat cacgacgata caacgcctcg aggtcggccg
     3601 tggtgatgac gagcaaacga gaaacggcga gagaacgaac ggaagcagaa cgcggttgca
     3661 catcgaggat ggacatctcg ccaaaccaat cgccggttcc gagcagcgcc acacgggtat
     3721 cgacaccccg tttggagttt ttgagcacct cgaattcccc atgcaacacg acatacattt
     3781 cgctggaggt atccccttcc cgaaagataa gttcaccggg ag
//
LOCUS       DAOSOR010000559         3817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_24024_length_3817_cov_3.622275, whole genome shotgun sequence.
ACCESSION   DAOSOR010000559 DAOSOR010000000
VERSION     DAOSOR010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24024_length_3817_cov_3.622275"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..1355)
                     /locus_tag="PK710_21615"
     CDS             complement(147..1355)
                     /locus_tag="PK710_21615"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT12392.1"
                     /translation="MSRPPRTVQELLLVCTAVSGMLLAVECRLSDSLDDFSRGGPTAG
                     FGGGGYGGESTDGGIQCEKYPVRCDEFAGDSARLMGCCEDNTHWWCERVQGDWRLRSK
                     DCGSNAGCGYDANTGALRCISGQGGSGGGETGGTGGETGGTGGTTGGTGGSGGCSPEC
                     DAKLEECGIHGYCVAKSVPMPGGYSIDATEVTRSQYEAWFLTNPSTAGQPAACSSWNK
                     TYQPDLACMNSDRVYKGPNSGNHPVVCVDWCDAYAYCKGVGKRLCGKIGGGANDYGDF
                     TDVSKSQWYAACSSGGKYEFPYGATYDGQACNSSHKELGTTSEVGSLSSCQSSASGYE
                     GVYDLSGNVWEWEDSCNGTNGISDYCRVRGGAFDFYGNYLGCGNDGRYVRGGSESFLP
                     SAGIRCCSLP"
     gene            complement(1997..>3817)
                     /locus_tag="PK710_21620"
     CDS             complement(1997..>3817)
                     /locus_tag="PK710_21620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12393.1"
                     /translation="TCGQLAAAGPARLSGIAQQVVTIKVPLSGTGETDLPTELSFKVQ
                     SFDIKKKSVKIDPKWTPSWGAEITNATLTTTYDNQDTWAYFSGKIDVSATRNGCSYEL
                     SVPIDFTHVDMSCSTASDCNNPITDLCEGGACVKPECIGEPMLWGQFYTSCYGGTSYE
                     NHLCRTLGNVGGVCADEWCISYELVNGSCSIGRICDGGTCVPPGGALVGASCTRTLSD
                     SGCEAGALCDQGICRWACLEGEPGPHCPAGEVCLSTVCKAASDFAALGAPCSNSGDPC
                     APSGENLVGICRAGICKLRCAKNADCAANEICDTYEKICTPTKCGDGVVDPNAPYEEK
                     CDDHNQTDGDGCNRYCKKEAAQNSDCTDAATVIEGTQSIALTPGPLTMNCSSSGGTND
                     YGATIRFVAPAAGTGDIRFTHQGSSSTDYPQLADLVGCPGTIRYCSGTVGSSGTSTWS
                     GKSFTAYSPNQELWFAVRGRKPTGTVKVTFKPYSCVGIPLPVGDTVMTGPSDSNIFLK
                     PSFNLCEAPYHNRRLATFTAEKDGTVVLTLKTVGASLRAFEGQCPSQSQQDLACMSYS
                     ASQTNPASVSFPVTAGKQYGVITGAGSSAPMDITVHRAGP"
BASE COUNT          787 a         1154 c         1022 g          854 t
ORIGIN      
        1 gattcccgct cgaccatcgt aatcccacgc aagatcgtcg gggtccgaat atagcttaag
       61 aaccgacgga aacgcaatga cctgattcca ggccttgcgc atgctattca caagttcgct
      121 tatgctgtag ttttgcctgc aacaaatcag gggagggagc agcaacgaat cccagcgctg
      181 ggcaagaagc tttcgctacc gccccggacg tagcgcccgt cgttgccgca accaaggtag
      241 ttgccgtaga agtcgaaagc gcctccgcgc acgcgacaat agtctgatat tccattcgtt
      301 ccattacacg agtcttccca ttcccaaaca ttaccgctca aatcgtatac cccctcatac
      361 cctgaagcag acgactgaca actcgaaagg ctccccacct ccgacgtcgt tccaagttcc
      421 ttgtgagagc tgttgcacgc ttgtccatca taggtggccc cataggggaa ttcgtatttc
      481 cctcctgaag aacatgccgc gtaccattgg cttttcgata catcggtaaa atcaccgtag
      541 tcgtttgctc ccccgcctat cttgccgcac aagcgtttgc ctacgccctt gcaatatgcg
      601 taagcgtcgc accaatccac gcacaccacc gggtgattgc cgctgtttgg ccctttgtaa
      661 acacggtccg agttcatgca agccagatca ggttgatagg tcttgttcca actgctacac
      721 gctgctggtt gccccgctgt cgagggattc gtcaaaaacc acgcttcgta ctgactgcgc
      781 gtcacttcgg tggcatcgat ggaataacct ccgggcattg gtaccgactt cgccacgcaa
      841 tatccgtgga taccacattc ttccaacttc gcatcacatt cgggtgagca gcctcctgag
      901 ccgcccgtac caccggtcgt acctccggtt ccgccggtct ctccgcccgt gccgccagtt
      961 tcccctcccc cactgcctcc ctgtccggat atacaccgta acgcgccagt gttggcatcg
     1021 taaccgcacc cagcgttgct cccgcaatcc ttcgagcgaa gcctccagtc tccctgcacc
     1081 cgctcgcacc accaatgcgt attatcttcg cagcagccca taagacgtgc tgaatcacca
     1141 gcaaactcat cgcagcgcac agggtacttc tcgcactgga ttccgccatc cgtactttcc
     1201 ccgccgtaac ctcccccgcc gaaacctgcc gttgggcccc ctcgggagaa atcatccagc
     1261 gagtcgctca accgacactc cacagcaagg agcatgccag ataccgcagt acaaaccaac
     1321 aacaattcct gcaccgttcg gggggggcga ctcatgttga ggttttggtc catcacggct
     1381 cctcgcaatc gattcctgtg aaatctgtca aaatacaaca aatgctcaag gctcgccgag
     1441 aagagtgttg agtatagcaa ttgcgcgcca gacgcacaat tcccaattcg taaccatcca
     1501 agcaagaccg cttgttcgtc cggttaccct cgtttttccc gaattggaaa gggataggag
     1561 ctgcatattc ctgggtcttg atttgggctc ggtccttgac cccccttgag ccaccgccaa
     1621 cctgagccat cccctcctat tgtcccgagt atttcccggg tatgatccat gacccggtca
     1681 gacaacacct cgatgggaag aggcttggaa cgcagttacg aggcggtttg cgagggcagg
     1741 ttgatcccag aattcgcccc accgtccgac gagggtcagc atgcgaaggt tgaagacggt
     1801 ctgagccgtt tcagcgtagg tttcgcgccc cttccattcg ttaagtggca gtccagcgcg
     1861 actcccgcgt agataactcc agctcgcgaa atcagtcgag ggggagtgga cggaacctgg
     1921 atcgagcctc cgaccccggc cctggatacc atgcccatag atatataatg tttttatttt
     1981 cgataggtta ccacatttat gggcctgctc gatgaacggt gatgtccatg ggcgcgctgc
     2041 tgcctgcgcc cgtgattacg ccgtattgtt tacccgcggt cacagggaag ctcacggacg
     2101 ctggattcgt ctgcgatgct gaatacgaca tgcatgccag gtcttgttga gattgcgatg
     2161 ggcattgccc ttcgaaagct cgcaaggaag cccccaccgt tttcagcgtc agcacgacgg
     2221 tgccgtcttt ttccgcggta aaagtggcga ggcgacggtt atggtagggc gcttcgcaaa
     2281 ggttgaatga cggtttcaag aagatattgc tatctgacgg cccggtcatc accgtatctc
     2341 cgactggtag cggaatgccc acgcaagagt agggtttgaa tgtgaccttg accgtcccgg
     2401 tgggcttacg accgcggacg gcgaaccata gttcctggtt gggagagtag gcggtgaagg
     2461 acttgccgct ccaggtgctg gtgccggaag agcccacggt gcccgagcag taacggatgg
     2521 tgccaggaca acccacgaga tcggcaagct ggggatagtc cgtgctggat gaaccctgat
     2581 gcgtgaacct gatgtcccct gtgcccgctg ccggagccac gaatcgaatg gtggcgccgt
     2641 agtcgttggt tccacccgag ctgctgcaat tcatggtcaa cgggcctggt gtcaaggcga
     2701 tcgactgtgt tccctcgatg acggtggcag cgtccgtgca gtcggagttt tgggcagctt
     2761 cctttttgca ataacgatta cagccgtcgc catcggtctg attgtgatcg tcgcattttt
     2821 cctcataggg cgcgttgggg tcgacgacgc catcgccgca tttcgtggga gtgcagattt
     2881 tctcataggt atcgcaaatc tcgttggcgg cgcagtcggc gtttttggca catctcaatt
     2941 tgcagatgcc ggcacggcag attcccacga gattctcgcc gcttggagca catgggtccc
     3001 cactattgga acaaggtgcg ccgagggcag cgaagtccga cgcggctttg cagacggtcg
     3061 agaggcagac ttcgcccgcg ggacagtgcg gaccaggctc cccctcgaga caagcccatc
     3121 ggcaaattcc ttggtcgcac aacgcgccag cctcacagcc actatcgctc agcgttcggg
     3181 tgcagctagc gcccacgagc gccccgcccg gtggaacaca ggtgccgccg tcgcagattc
     3241 taccgatgga acaagagcca ttgacgagtt catagcttat gcaccactca tccgcgcaaa
     3301 cgcctccgac gttgcccaag gtgcggcaaa gatggttctc gtaggaagtt cctccgtaac
     3361 aactcgtgta gaattgtccc cagagcatgg gctcaccgat acactccggc ttgacgcatg
     3421 ctccaccctc gcacaagtcc gtgatcgggt tgttgcagtc ggaggccgtc gagcaactca
     3481 tgtcgacatg ggtgaagtcg atgggaaccg atagctcgta cgagcaaccg ttccttgtgg
     3541 ccgatacatc gatctttccg ctgaaatagg cccacgtatc ctggttatcg taggtggtgg
     3601 tcagtgtggc gttggtgatc tcggctcccc aggagggagt ccattttgga tcaattttca
     3661 cgctcttttt cttgatgtcg aaggactgca ccttgaagga aagctccgtg ggcaagtcgg
     3721 tttctccggt gccgctcaat ggtaccttga ttgtaaccac ttgttgtgcg attcccgaca
     3781 ggcgggctgg gcccgcggct gccaattgcc cgcatgt
//
LOCUS       DAOSOR010000560         3814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_24046_length_3814_cov_3.429370, whole genome shotgun sequence.
ACCESSION   DAOSOR010000560 DAOSOR010000000
VERSION     DAOSOR010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24046_length_3814_cov_3.429370"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="PK710_21625"
     CDS             complement(<1..992)
                     /locus_tag="PK710_21625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12394.1"
                     /translation="MRASRTVLIIFVLCFVGYAFLYVPRLANPLWSDVEFTGWVAPIA
                     WRMVQGQQIYRDFTLPISPGSFALMAGFQALTGRFFLLDELWLCALCQMLMIPIGYAL
                     VRPFTTERNACLATMAMVPVLISAPKEIAYDHTALVLAWSSLLWMSRGLLRSSGRRRE
                     ILLVASGLTAGLTLVFKSSTGMGAIGALGLAVLTMAWIGHKTEGRSSHRQVVREAMLV
                     GGGMLVGVLITLAVVVVLGGAIGEFVSVVFVDGPRLKGGKGQAILNLLSYTIVQTPIH
                     VSFLLGAVLASVIARAVVRNPSFSIVNISDPPEENGRRAWVFAVGMSAYVLLVF"
     gene            complement(1037..1822)
                     /locus_tag="PK710_21630"
     CDS             complement(1037..1822)
                     /locus_tag="PK710_21630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOT12395.1"
                     /translation="MACRRGDGFRVQEHRGAAIFVMDRPDKANALTRAMLEGLGAFSR
                     EMVAREDIRALVIVGAGDRYFCAGADLRERRGYSQDDVREQLRLYRSELGVLDTSPKV
                     VVAGINGMALGGGLEIAMACDLRVASPHAVLGQPECALGIIPGAGGTQRLPRLVGEAR
                     AKEMILLGRRLTSEQALAWGLVNRIESGDLLEGILDWLRPVLEGAPLAQAAALEAMAA
                     SHLPLSRGLEVEAMAYERVLGSEDRMEGLLAFEQKRAARFVGR"
     gene            2049..2672
                     /locus_tag="PK710_21635"
     CDS             2049..2672
                     /locus_tag="PK710_21635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12396.1"
                     /translation="MSQGQTPPTPRESALVFAEGARSGWIASDLIAWMNEHLIAPKRL
                     DTRDGRVHQVVEHGCPTIVFNGATPITPAIRTQTASQVPSLVASARERVIHALRATVR
                     TGETSFVNTALYAGRVARERGPLSKPHWHVYVTEDDALSDQVLALFAADALTHPVDYE
                     RNIAVCDVCGAIVFSQSPSRHGCEAHPFGAVEPRSGHWTHSRTNARS"
     gene            2704..>3814
                     /locus_tag="PK710_21640"
     CDS             2704..>3814
                     /locus_tag="PK710_21640"
                     /inference="COORDINATES: protein motif:HMM:NF025843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOT12397.1"
                     /translation="MTSGRLTHPEEPTSTLQGSIERITFENETTGFRVLQVAPEPEGP
                     SVTVVGPMQPMSLGSRVRITGRWENDARHGLQLRAHTVLLLEPDTREGLMRYLGSGLI
                     PGIGPAYAQRIVDRFGEDTLRVLDQEPHRLEEVQGLGPRRALAVSQAWTERRALHDAM
                     VFLQSHGVTSSLAIRIFRKFGADTIRVVSENPFQLATEVWGIGFQKADQIATMLGIAK
                     QAPQRLEAAVLFALHQAEERGHVFLPRHELTRAAAQIANVPEEHIPIALERTLLTNAR
                     QDTIEPTGPVVYRNQRFIEEAALAHGLARIHGASVAPLTKANDAIAAFEHHTGIELAD
                     EQRQAIQQAAQSPLLIITGGPGVGKTTLLRAILELF"
BASE COUNT          910 a         1393 c          864 g          647 t
ORIGIN      
        1 tcaaatacga gcaaaacgta cgccgacatg cccaccgcaa atacccaggc ccttcgtccg
       61 ttttcttccg ggggatctga gatattgaca atggaaaacg aggggttacg cacgacagcc
      121 cgcgcgatca cgctcgccaa gaccgccccc aacaaaaacg atacgtgaat cggcgtctgc
      181 acgatcgtgt acgacagcaa gttcaaaatc gcctgccctt tgcccccttt gagacgtggc
      241 ccatcgacga aaaccaccga tacgaactcg ccaatcgccc cccccagcac cacgacaacc
      301 gcaagcgtga tcaatacccc caccaacatc ccaccaccca cgagcatggc ttcacgcaca
      361 acctgtcgat ggctcgatcg cccttccgtt ttatgaccga tccacgccat cgtcagcact
      421 gccaagccca acgcgcctat cgctcccatg cccgtgctcg acttgaatac aagcgtcaat
      481 cccgccgtta accccgatgc cacgagcaag atctcgcgac gccgtccgct agaccgcaac
      541 aacccccgac tcatccacag cagactgctc caagccagca ccaaagccgt gtgatcgtag
      601 gcaatctctt tgggcgccga gatcaacacc ggcaccatgg ccatcgtcgc aagacaggca
      661 ttgcgctccg tcgtaaaagg acgcaccaac gcatagccaa tcggaatcat caacatctga
      721 caaagcgcgc acaaccacag ttcgtcgagc agaaaaaatc gccccgtcag cgcttgaaaa
      781 ccagccatga gcgcaaagct tcccggggag atgggcagcg tgaaatccct ataaatttgc
      841 tgcccttgca ccatgcgcca cgcaatggga gctacccaac cggtgaactc cacatcactc
      901 cacagcggat tggcaagacg gggaacgtaa aggaacgcgt agcccacgaa acacaaaacg
      961 aagatgatga gaaccgttcg cgaggcacgc acgaactgca acctagagaa catgagccga
     1021 ccacgccaag aaaaagtcag cgccccacga atcgtgcagc acgtttttgc tcgaatgcca
     1081 acaacccctc catccgatcc tcactgccta gcacccgctc atacgccatc gcttccacct
     1141 cgagtccccg cgataaaggc aaatgcgacg ctgccatcgc ttccaaagcc gccgcttgcg
     1201 ccaaaggcgc tccctccaac accggcctca accaatccaa aatcccttcc aataaatccc
     1261 ccgactcgat ccgattcacc aatccccatg ctagcgcttg ctcgctcgtg aggcgacgcc
     1321 ccagcaaaat catctccttg gcacgagctt caccaacgag tcgcggcaat cgctgcgtcc
     1381 ctccagctcc cggaatgatg ccaagggcac actccggctg ccccaatacc gcatgcggac
     1441 ttgctacccg aagatcacaa gccatcgcga tctccaaccc ccccccaagc gccatcccgt
     1501 tgatccccgc caccaccacc ttcggactcg tatcgagcac cccgagttcc gaccgataca
     1561 atcgaagctg ctcacgaacg tcgtcttgcg aatacccacg ccgctcacgc aaatccgccc
     1621 cagcgcaaaa atatcgatcc cccgccccca cgatcaccag cgcacgaata tcctcccgag
     1681 ccaccatctc ccgcgaaaat gccccgagcc cctcgagcat cgcgcgggtc aatgcattcg
     1741 ccttgtccgg gcgatccatt acaaaaatcg ccgctccccg atgctcctgc actcgaaaac
     1801 catccccacg tcgacaagcc atgccccctg ctaccaacac tgccttggta tcgccagtcg
     1861 atccgtcccc atgagccatt tattccacgt ttttcgttcg taataaaaaa aactagcgac
     1921 cgactgatca atcgtgctaa agaaatggac ttgcataagc gtgaatctca tgcttcgcct
     1981 caccaggggt tcttgaattg ccaagaccgt ctacccgggg gcaccctgtg gaaaacaagg
     2041 gggacatcat gagccagggc caaacaccac caacaccgcg agagtcggct ttggtctttg
     2101 ccgaaggcgc ccgctcaggt tggatcgcca gcgacctcat cgcctggatg aatgagcatc
     2161 tgattgcacc aaaacgcctc gatacccgag acggacgcgt gcatcaggtc gtcgaacacg
     2221 gctgccccac catcgttttc aacggggcaa cgccgatcac cccggcgatt cgtacacaaa
     2281 ccgcttccca agtgccctcc ttggtcgctt ccgcccgtga gcgcgtcatc cacgctttgc
     2341 gagctaccgt ccgaaccggg gaaacaagct tcgtcaacac cgccctatac gccggtcggg
     2401 tagcccgaga acgcggcccc ctcagcaaac cccactggca tgtctatgtc accgaagacg
     2461 acgccctcag cgatcaagtc ctggccttat ttgctgccga cgccctcacc catcccgtcg
     2521 attatgaacg gaatatcgcc gtatgcgacg tctgcggagc cattgtcttt tcccaatccc
     2581 cctcacgaca cggctgcgaa gcccacccct tcggcgcagt cgagccccgc tccggccatt
     2641 ggacacactc gcgcaccaac gctcgatctt gacgcgccat cccttgaata cgacgctgcc
     2701 tccgtgacat ccggtcgcct gacccatcca gaagaaccca cctcgacact ccaaggctcg
     2761 atcgaacgca tcacgtttga aaatgaaacg accggattcc gtgtcttgca ggtcgccccc
     2821 gaacccgaag gcccctccgt cactgtcgtc ggtcccatgc aacccatgtc cctcggaagc
     2881 cgtgtgcgca tcaccggacg atgggagaac gatgcccgcc acggcttgca actgcgcgcc
     2941 cacaccgtcc tgcttctcga gcccgacact cgggaagggt tgatgcgtta cctgggctca
     3001 gggctcattc ccggtatcgg accagcctac gcacaacgaa tcgtcgaccg cttcggagaa
     3061 gataccctgc gggtgctcga ccaggagcct catcgactcg aggaagtcca aggactcgga
     3121 ccacgacgcg cccttgcggt atcccaagct tggacggaac gacgagcgtt gcacgacgcc
     3181 atggtctttt tgcaaagcca cggcgtcacc tcctcccttg ccatcaggat ctttcgcaag
     3241 ttcggtgcgg ataccatccg tgtggtatcc gaaaacccat ttcaattggc caccgaagtg
     3301 tgggggatcg gctttcaaaa agccgaccaa atcgccacga tgctgggcat cgccaaacag
     3361 gccccccaac gactcgaagc agccgtgcta ttcgcgctgc atcaggcgga agagcgcggc
     3421 cacgtctttt taccccgcca cgaactcaca cgcgcggccg cccaaatcgc taacgttccc
     3481 gaagaacata tccccatcgc cctcgaacga actctgctca ccaacgcccg tcaagacacc
     3541 atcgaaccca ccggccccgt cgtctaccgt aaccaacggt tcatcgaaga ggccgccctt
     3601 gctcacggtt tggcccgaat acacggcgca tcggtcgccc ccttgaccaa agccaacgac
     3661 gccatcgccg cgttcgaaca ccacaccggc atcgaactgg ccgacgaaca gcgtcaagcc
     3721 atacaacaag ccgctcaatc cccgttgctc atcatcacag gaggccctgg cgtcgggaaa
     3781 acgaccctcc ttcgcgccat tctcgaacta ttcg
//
LOCUS       DAOSOR010000561         3803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_24139_length_3803_cov_4.038687, whole genome shotgun sequence.
ACCESSION   DAOSOR010000561 DAOSOR010000000
VERSION     DAOSOR010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24139_length_3803_cov_4.038687"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PK710_21645"
     CDS             <1..736
                     /locus_tag="PK710_21645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12398.1"
                     /translation="QDHGEGKGTMSRAEAIVAASAIMISDNPTAQNLRKNLASLRQIP
                     LFERLLATTPTNTDAALSGELAPVPRGPVATFFLGMIGWLLISHVVRLIGRFALQLRR
                     PAELTITTTGIHVRSTTQLLGKVLRESETRIPWEGLVRATREVRYPRLGTYAGLTALA
                     LGSYIGVGWFIDGIRAQSLSLATMGLLVVVLGVALDFVLVSLLPGRKGKCRLVLVPRK
                     GAATCVGWVDATKADALLRQLDRGQP"
     gene            950..2065
                     /locus_tag="PK710_21650"
     CDS             950..2065
                     /locus_tag="PK710_21650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12399.1"
                     /translation="MNLDIRTNSIRIVLLSCLVHSLGCSGDGGDASASLGIDASGSGG
                     AGGTQTGEASTDSFAEASIDSREDMASEGLVSDSFGEEDPDVLSADVSDAEHDSTTDV
                     IDSGEDTAEDTGVDAPGDSDMDSGFDTGTDAMADSSVDSGFDTGTDAMADSSADVPLD
                     ITQDAPPPCTSLGEYCSASAQCCLPESGTSACRRTICALGPGTCLLDDAACLRDWECC
                     SGVCENGVCAPPFCLDDGQHCSDDWDCCSNVCYEGVCIAPMCDENGVACSEDESCCSY
                     YCGPGGTCENAPCQPVGASCAVSEDCCSFYCNSAGLCDEQCAALGSSCVANQDCCSNV
                     CEQNRCVPPQCLANGEYCIDDAQCCSFRCDETYTCVP"
     gene            complement(2077..3612)
                     /locus_tag="PK710_21655"
     CDS             complement(2077..3612)
                     /locus_tag="PK710_21655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12400.1"
                     /translation="MEKKTQRRGSWDTPVLGHLVAVVISLLIHVLPLPVYLLGGAFSF
                     FGPAAEELEDHETVIPIDLMLSEGDEPATVAQALPPLTLESDPTSIPTMPIVVDAGTA
                     VSVDAAPESTDADTPSSADSGFDVLVALDSSDAAEAFDALEALDAKRMDGALEADVVE
                     KSENDAALVSEAASPMPGALDADIARADRLDASVAMAAPDAAHEAGNVGIEGGSGEAR
                     VSRPIRDPIGLAGDARRIAPRDPNVSVLIYPERIRSHPLAIQFAPTLTKLRNWRNFFG
                     GTDLDPIRDTDRILLAGPQLRDSSRVVAVVRYNVPQQRVRSAIDSVVQRNRGQWETKS
                     VPVAKAHVDGAERYFVMTGPQMLVIVPPDGLAQALQLPRNLRFPQGGQEAVVLFLRYP
                     ANAFRGLPVKLPTSVEWMRFSLSINPSGGADAKLEAKDRDAESASTNAPELTKIINKA
                     MVVDLLFTQRRLLDPVTFRAEGDRIRAETHVTDNQLRHILSLMAAKIEQVDKEAPSPA
                     GSR"
BASE COUNT          793 a         1079 c         1097 g          834 t
ORIGIN      
        1 ccaggaccat ggggaaggaa aaggaacgat gagccgggcg gaagccatcg tcgccgcatc
       61 cgcaatcatg atcagcgata atccaaccgc ccaaaacctg cgaaagaatc tcgcctccct
      121 tcgccaaatc cctttgtttg agcgtctgct cgccaccaca cccacgaaca cggatgctgc
      181 cctgtccgga gaacttgcgc cggttcctcg cggtcctgta gccacatttt ttctcggcat
      241 gatcggttgg ctgctcatct cccacgtcgt tcggctcatc ggacgtttcg ctttgcaact
      301 tcgccgaccg gcggaactca ccatcacgac cacgggaatc cacgtgcgtt ccaccaccca
      361 actgctcgga aaagtgctcc gtgaatcgga aacacgaatt ccatgggagg gcctcgtgcg
      421 cgcaacccga gaagtccgct acccgcgtct gggcacttac gccggcctga cggccctcgc
      481 tctcggaagc tacatcgggg tgggatggtt catcgatggg attcgagccc aatccctgtc
      541 tctcgccacc atgggattgt tggtcgtcgt gctgggagtc gccctcgact tcgtcctcgt
      601 cagcctattg ccgggtcgca aaggcaaatg ccgcctcgtg ttggtgccgc gaaaaggcgc
      661 tgctacgtgc gtaggatggg tcgatgccac gaaagccgat gccttgctcc gccaactcga
      721 ccgcgggcaa ccgtagccta taaccctttt ttcccatcct cgacacacca cgctctccat
      781 ccatcggtct ccgatattta ataatatatt caaatactta ccaaggcatc atcctctttt
      841 tcttgcccat gtcccgccag actcgatgtt ctatccataa gccacttatt caaatttcta
      901 ctatattcca tgataccgct ttcgaacaaa aatattataa cgtgaattcg tgaacctcga
      961 cattcgaact aactccattc gtatcgtttt gctttcgtgc ctcgtccatt ccctgggatg
     1021 ttcaggggac ggaggtgatg catcagccag ccttggcatc gatgcctcgg gctccggggg
     1081 cgctggaggc acacaaaccg gggaggcgtc gacggatagc tttgccgaag catctatcga
     1141 ttcacgagag gatatggctt cggagggtct ggtcagcgac tcgtttgggg aagaagatcc
     1201 ggacgttctt tccgctgacg tttcggacgc ggagcacgac tcgacgacgg atgtcatcga
     1261 ttcgggcgag gacacggcag aggatactgg tgtggacgca ccaggggact cggacatgga
     1321 ttcaggattc gacacgggca ccgatgcgat ggcggattcg agtgtggatt cagggttcga
     1381 cacgggcacc gatgcgatgg cggactcgag tgccgatgtt cccctcgaca tcacgcaaga
     1441 cgcgccgccg ccatgtactt ccttgggtga atactgctcg gcctccgcac aatgttgttt
     1501 gccggaatcg ggcacgtccg cgtgtcgtcg caccatttgt gcgctcggcc cgggcacgtg
     1561 tttgttggac gacgctgcct gtttacggga ttgggagtgt tgttctggcg tttgcgagaa
     1621 tggggtttgc gcaccgccat tttgcctcga cgatgggcag cattgctcgg acgactggga
     1681 ttgttgctcg aacgtttgtt acgaaggggt atgcattgct ccgatgtgtg atgagaacgg
     1741 cgttgcatgc agcgaggatg aatcatgttg ttcgtactat tgcgggccgg ggggaacgtg
     1801 tgaaaacgcg ccttgtcaac cggtcggggc ttcatgcgct gtgtctgagg attgctgctc
     1861 gttttattgc aattcagcgg ggttatgcga tgagcaatgc gccgcacttg gaagctcatg
     1921 tgttgcgaac caggactgct gttcgaacgt gtgcgaacaa aatcgctgtg tgcctcccca
     1981 atgccttgcc aacggggagt attgcatcga tgacgcacaa tgctgttcgt ttcgctgcga
     2041 cgaaacgtat acgtgcgttc cttgagggcc tgacagtcag cgagatcccg ctggacttgg
     2101 agcctctttg tccacctgtt cgattttcgc ggccatcaag ctgagaatat ggcgcaactg
     2161 attgtccgtc acgtgagttt cggctcgaat acggtcccct tcggcgcgaa acgtgacggg
     2221 atcgagaagg cgacgctggg taaagagcag gtctaccacc atcgccttgt tgatgatttt
     2281 ggtcaattcg ggtgcattgg tcgaagcgga ttcggcgtcg cggtccttgg cttccagttt
     2341 tgcatcagct cctccggaag ggttgatgct caacgaaaag cgcatccatt ccacgctggt
     2401 gggaagcttc acgggcaggc cacgaaatgc gttggcgggg tagcgaagga agagcacgac
     2461 cgcttcctgc ccgccttgcg gaaaacgaag gtttctaggt agttgcaacg cttgtgcgag
     2521 gccatcgggg ggcacgatca ccagcatctg gggccccgtc atgacgaaat atcgttccgc
     2581 cccatcgaca tgcgctttgg ccacgggaac gcttttcgtt tcccattgcc cccgatttcg
     2641 ttgaacaacg ctatcgatcg ccgaacgaac gcgctgttgt ggcacgttgt aacgaacgac
     2701 tgccacgaca cgggagctat cgcgcaactg cggaccagcg agcaagatgc ggtcagtatc
     2761 gcgaatgggg tccaaatccg taccgccgaa aaagttccgc caatttcgga gcttcgtcag
     2821 ggttggcgca aactgaatgg caagagggtg agaacgaatg cgctccggat aaatgagcac
     2881 ggacacattg ggatctcgag gtgcgattcg cctggcatct cccgcgagac caatgggatc
     2941 tcgaatgggc cgagaaacgc gcgcttcacc acttccccct tcgatcccca cgtttcctgc
     3001 ctcgtgggcc gcatcggggg ccgccatcgc cacggaagcg tcaagcctat cggctcgcgc
     3061 aatatccgcg tctaatgcgc ccggcatggg agaagccgct tccgaaacaa gcgccgcatc
     3121 gttttcagat ttctccacga catccgcttc gagcgcgcca tccatccgtt ttgcgtccaa
     3181 tgcctccaat gcgtcgaatg cttccgccgc atcggacgag tccaaagcaa ccagcacatc
     3241 gaaacccgaa tccgcggagg atggagtatc ggcatcggtt gattcgggag ctgcatccac
     3301 gctcaccgcg gttccagcat cgaccacgat gggcatggta ggaatcgagg tggggtcgga
     3361 ctcgagggta aggggaggaa gggcttgcgc cacggtggcg ggctcatctc cttcactcag
     3421 catcagatca atcggaatga cggtttcgtg atcctccaat tcctcggcgg cagggccgaa
     3481 gaaagagaac gctccgccca atagatacac gggcaacggg agaacatgga tcaacaaaga
     3541 gatcacgaca gcgacaagat gcccaaggac aggcgtgtcc caagaaccgc gtcgctgcgt
     3601 ttttttttcc acgaactgga ccatagcgcg tgttcgttgg acgggcgaac ccctctttgc
     3661 ttgttgtttc cacgatggca tgagttgtgc gattcgccga tttgtggatc ggccggccca
     3721 gtcaggctac gaagacgggc aacgggataa tcagatgaat cgacaatccc gaggaatcga
     3781 tggcaggaaa cgtgaaacag aca
//
LOCUS       DAOSOR010000562         3793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_24237_length_3793_cov_4.530765, whole genome shotgun sequence.
ACCESSION   DAOSOR010000562 DAOSOR010000000
VERSION     DAOSOR010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24237_length_3793_cov_4.530765"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2851)
                     /locus_tag="PK710_21660"
     CDS             complement(<1..2851)
                     /locus_tag="PK710_21660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOT12401.1"
                     /translation="MIKKTLGIDIGSTSFKLCVLSSSPGAEPLAESHAILSHDGDIEG
                     TLERLLDTLGFEDTDEVVGLVTGNEGRHRIALPDVIAAVAIESALDALQLRPRAVVSM
                     GGEDLVVYVLDGRGRIINTYAGNKCASGTGEFFRQQLGRMNLELDDINDICRHACVHP
                     ISARCSVFMKSDCTHRLNKGEATKADVALSLSKVMADKVGEFLIKAKLAQGQVVLIGG
                     VTRNEHLIEFIGKSNPSIELIVPEQAAYFEAFGAAYLARLKGAKVPARDALVRPGVAL
                     AYKTFSPLSEASSLVFHAPSRRAAYDPDAEYILGVDGGSTTTKVALVNALTLEIVAEH
                     YGRTHGDPVAALKQCLREVRRQLGDHKPRIRLVATTGSSRELLGVFLETVGVYNEIIA
                     HTVGTTFFQKDVDTIFEIGGQDAKYVLINNGVPIDYAMNEACSAGTGSFLEESAAGDL
                     NIHSASEIGPIALRAAAPLKFGEHCSAFINSDIRKAIQQGAPREDIVAGLVFSIVANY
                     LNRVVGNRSVGSHIVLQGGVAKNPAVPLAFAQMTGKSITVPPDPELMGCFGVARLVLQ
                     KHDEGLVDKGEFDIDALIDKPIAYGKDFVCKSCDNLCTVRRLVVGARRYPFGGRCSLY
                     TNQRKKRTIHEEKVVDYTQIRTRMLFEDFAPKPDSLRLRTTRVVGVPMAFTVHSLWPF
                     YSWFFHTLGVRIVTSTRIVPEGIAKQESNYCFPTEIAHGAIQDVLNQGVDFIFLPHFR
                     DMPSTEDVHACVCPLTQGLPYFARQAFGLADEMVLRPVLSLKDGFEASRGAFENVAVQ
                     LGFTREEGSRAYDKGIEEYRRFLDAYRTLGKEVLREVQSNPDRVFIALLGRPYNAFTR
                     DANMGIPRKFTSQGVTILPFDMIYSEEGEIPPNNYWYYGQQNMRAVQQVKRVENLYLA
                     WISNFSCAPDSFLLHYVRWMMGQKPYLVLEIDSHTA"
     gene            complement(2985..3686)
                     /locus_tag="PK710_21665"
     CDS             complement(2985..3686)
                     /locus_tag="PK710_21665"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT12402.1"
                     /translation="MNGNKAPRKAGGAAAVAPKKPPSLRQKAKALYRQAILDAAERIF
                     ATQGIRGARIQDIAKLAGMSVGTVYNHFAQKEEIVVALITEREKELREAFACQPHDPQ
                     NFTEAFRIRHERVIQLIRTHKDFFSFALYEGIFESELVPSASMFSTHSADEHERFDRA
                     MEDLLEQGGREGAVAPTDVVRLKRYHVGAMRGVLMGAMKDPSRDVVEEGLAAVELFLR
                     AVRPNAKGIPLPDIV"
BASE COUNT          882 a         1150 c          979 g          782 t
ORIGIN      
        1 ccgcggtatg agagtcaatt tccagcacga gatagggctt ttgccccatc atccatcgca
       61 cataatgcaa taaaaacgag tccggcgcac aggaaaaatt cgaaatccaa gccaaataca
      121 ggttctccac ccgtttgact tgctgcaccg cccgcatatt ttgctgcccg taataccaat
      181 aattgttcgg cggaatctcc ccctcctcgg aataaatcat gtcaaagggc aagatggtca
      241 ccccttgcga cgtgaacttc cgtggaatcc ccatgttcgc gtcgcgcgta aacgcattgt
      301 agggccgccc gagcagcgca atgaataccc gatccgggtt gctctgcacc tcacgcaaca
      361 cctctttccc aagtgtacga taagcatcga gaaatcgacg atactcttcg attcccttgt
      421 cgtaggcgcg actgccttcc tcccgcgtaa acccaagttg cacagccacg ttctcgaacg
      481 cgccgcggct cgcctcaaac ccatctttca ggctcaatac cggccgcaac accatctcgt
      541 cagccaaccc aaacgcttgc ctggcaaaat acggaagccc ctgggtcaac ggacacacgc
      601 aagcgtgtac gtcctcggtg ctgggcatat cgcggaaatg tggcaaaaag atgaagtcca
      661 ccccttggtt gagcacatcc tgaatcgccc catgcgcgat ctccgtggga aaacaatagt
      721 tgctctcctg cttcgcgatg ccctcgggaa cgatgcgcgt ggaggtgacg atccgaaccc
      781 ccaaggtatg aaaaaaccac gaataaaagg gccaaaggga atggaccgtg aacgccatcg
      841 gcaccccgac cacacgcgtg gtgcgaagac gtagcgaatc gggcttggga gcaaagtcct
      901 cgaagagcat ccgcgtacga atttgcgtgt agtccacgac cttttcttcg tggatcgtcc
      961 gctttttacg ctggttggta tatagcgaac agcgaccccc aaaaggataa cgacgcgccc
     1021 ccaccaccag cctgcggacg gtgcatagat tgtcgcagga cttgcacacg aagtcttttc
     1081 catacgcgat gggcttgtcg atgagcgcgt cgatatcgaa ttcgcccttg tcgacgaggc
     1141 cctcgtcgtg tttttggagc acgagtcgcg ccacaccgaa acatcccatc agttccgggt
     1201 cggggggaac cgtgatcgat ttgccggtca tttgcgcaaa cgccagggga accgcggggt
     1261 ttttggcgac gcccccttgc aacacgatat ggctgcccac gcttcgattg cccacgacgc
     1321 gattcagata gttggccacg atggaaaaga cgagccctgc cacgatatcc tcgcggggag
     1381 cgccttgctg aatggctttt cgaatatccg agttgatgaa cgccgaacaa tgctcaccga
     1441 acttcaaagg cgccgcggcg cgaagagcga tgggaccaat ctccgaagcg ctatggatgt
     1501 tgagatcgcc cgccgcggat tcctcgagaa aggaaccggt ccccgccgaa catgcttcgt
     1561 tcatcgcata atcgatgggt acaccgttgt tgatcaacac atacttggca tcctgcccgc
     1621 caatctcgaa tatggtatcc acatctttct gaaaaaaagt cgtgcccacc gtgtgggcaa
     1681 tgatttcgtt gtatacaccc acggtttcca aaaatacgcc aagcaattca cgcgaagaac
     1741 ctgtcgtcgc cacaagacgt attcgcggtt tatgatcgcc aagctgacga cgcacctccc
     1801 tcaaacattg cttgagagcc gccaccggat caccatgggt gcgaccgtaa tgctccgcca
     1861 cgatctcgag cgtcaacgcg ttgacaagcg ccaccttcgt cgtcgtcgaa cccccatcga
     1921 ccccaaggat atattccgca tccggatcat acgccgcacg acgagaaggc gcgtgaaaaa
     1981 ccagactcga tgcctccgac agaggcgaga aagttttgta agccagggca acacccgggc
     2041 gaacaagagc gtcccgcgca ggaaccttgg cccctttcaa ccgcgcgaga taggcagcgc
     2101 caaacgcttc gaaatacgcg gcctgctcag gcacgatcaa ctcgatcgaa ggattagatt
     2161 taccaataaa ttcaatgaga tgctcatttc gcgtcacgcc gccgatgagc acgacttgcc
     2221 cctgcgcaag cttggctttg atcagaaatt ctccgacctt atccgccatc accttactca
     2281 gtgatagcgc aacatccgct ttcgtcgcct cccccttgtt gagccgatgc gtgcaatccg
     2341 acttcatgaa caccgaacaa cgagcggaga tcggatgaac gcaagcatga cgacaaatat
     2401 cgttgatatc gtcgagttcg agattcatcc gccccaactg ctgacggaag aactcccccg
     2461 ttcccgaggc gcatttattt cccgcgtagg tattgatgat acgacctcga ccatcgagca
     2521 catacaccac caaatcttcc ccacccatgg aaaccacggc gcgaggacga agttgcaacg
     2581 cgtcgagggc ggactcgatg gccaccgccg caatgacgtc gggcaacgca atgcgatgac
     2641 ggccttcgtt gcccgtcacc agccccacga cctcgtcggt atcctcgaaa cccaacgtgt
     2701 cgagcaaacg ctccaacgtg ccttcgatat ccccgtcgtg ggaaaggatg gcatgagact
     2761 cggcgagagg ctcagcgcct ggcgacgacg acagcacgca tagtttgaaa gaagtggatc
     2821 cgatgtcgat tccgagggtc tttttgatca tggtgactgc caaaactgca aaggactacg
     2881 tgaaaggaca acgtgccgat cggcgcccgc tgggttagcc caacgtggga gagttggcaa
     2941 tcaggcaggg aggggtgaga ataaaaatga ataaatacaa ttggttatac tatatccggc
     3001 aaggggattc ctttcgcgtt tgggcgaacg gcgcgcaaaa ataactcgac cgccgccagg
     3061 ccttcttcga ctacatcgcg gctcggatct ttcatcgccc ccatcagcac ccctcgcatc
     3121 gcccccacat gatagcgttt gagccgaacc acatcggtag gtgccacggc accttcgcgt
     3181 cccccctgct ccaacagatc ttccatggcc ctgtcgaatc gttcgtgctc atcggccgaa
     3241 tgggtggaga acatcgatgc cgacggaacc agttcggatt cgaaaatccc ttcgtagagc
     3301 gcgaaagaaa aaaagtcctt atgggttcgg atgagctgga tgacacgctc gtggcggatt
     3361 cgaaacgctt ccgtgaagtt ttggggatcg tgtggttggc aggcgaacgc ttcacggagt
     3421 tctttttccc tctcggtgat gagcgccacc acgatttctt ctttttgggc aaagtggttg
     3481 tagacggttc cgacggacat acccgcgagt tttgcgatgt cctggatgcg cgcgccccga
     3541 atcccctggg ttgcgaagat ccgttccgcg gcatcgagga tcgcctgacg ataaagcgct
     3601 ttggcttttt gtcgaagcga ggggggcttt ttcggggcta ccgcagcggc ccctccagct
     3661 tttcgcggcg ccttgttgcc gttcatctta cgctccttca tccaccttgg ctgaggaggc
     3721 atccaccacg ggcatttttg ctgctttttt tccgcggctc cacaacgctc ccaaccgagc
     3781 ccgcaccccg tcg
//
LOCUS       DAOSOR010000563         3786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_24290_length_3786_cov_4.806218, whole genome shotgun sequence.
ACCESSION   DAOSOR010000563 DAOSOR010000000
VERSION     DAOSOR010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24290_length_3786_cov_4.806218"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1720)
                     /locus_tag="PK710_21670"
     CDS             complement(<1..1720)
                     /locus_tag="PK710_21670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12403.1"
                     /translation="MMEYRTADIEDLERVLASAPSPFSDSVLAHALRTPLDVPTIHAS
                     LRQHLLGLVGPCAEERQPVFQVITGDPGEGKTHLLAWLRQASEDSLCSTDGQFFALAP
                     IEPLRAFHRIFHHFLHETVRNLSRPLSVSGHLDSAVGSPLAVIVLRALLRIARLVRWA
                     PWTPPDLVETLDKVIPNRPSFFLGAFAEVAACCWGDIERSFVLSAQRLDALAGMDLEL
                     FRVIAGFPHPDLRQDLIAWLGGVSLSEQAATRLGTRIILDDEQNAWRGLRLLVDLAAL
                     ADVPLALAFDQVEGTERLGEEAVAAWLTALGELYNLGGPTVLLMLCQTQTWPRLRQRA
                     PHHVRDRMEARAPIPLLALRPEQAVSLVELRMHRFWRGVGLAPANPAYPFESDALLDL
                     IRSNHLRSPRSILRFMRQWLDDRAGGTHSKPAEPRPIVRSEEIQRRLHAMIEDDRRRP
                     ERMPEVREQIVQSALREALTTAWLHGKTVFGARIENVDIPAMRTRSRAGCRITLLLST
                     KRLRLYFETNNSAHGQSVASATKRMRDMLRDEQADRVMLVRETALPLPPASRNMLQRL
                     YPRSLVF"
     gene            complement(1717..2334)
                     /locus_tag="PK710_21675"
     CDS             complement(1717..2334)
                     /locus_tag="PK710_21675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12404.1"
                     /translation="MTPQQERVLRTLLARGPVTQASLKADSSTVSELIERQLILSTPP
                     ARKGASARLNITAAGKQALHGEDGSQNATILQQLDTLRKQVADLHALVEAIAITVGAS
                     AVTEQPCQPALPRTLEQFESALRHACHELSTKGGHGALVPLPRVRESLISLGISREAF
                     DDALLALEEQFRIDLKVANDPERLTDRQHGIEVPNRGLLYFVVLR"
     gene            2802..>3786
                     /locus_tag="PK710_21680"
     CDS             2802..>3786
                     /locus_tag="PK710_21680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12405.1"
                     /translation="MGNIQTRGHVEELAISKLEAHGWTVVGGERRGVDLDLFVQFDGI
                     SGKVFFRNAFNLSQGMAETANSDPCSVWIELGEPVGEPTWWSLWDQYRFGQGSKEHAF
                     IATIIHEAIGIGWTKVAGGDVEPGQTWRSPSKTLDTIISRAACLAPSRNEADRRYEIW
                     KKQRHNFGSPPKKHSLVLFLVLLAMPVGVMFFDWLDNGKLTIASIVGPVLLSILPFIF
                     LLDLLKTSKDHLRRPRNLVAGVDAVVNAMRITGVFDVFKVTTAKLRHGLPVVVRSHGY
                     LDRHHQPHGSPKSNTKGSPARAVGDKGIHINDTPHVRWMFATRRIGPVDSAT"
BASE COUNT          813 a         1022 c         1179 g          772 t
ORIGIN      
        1 agaaaacgag gctacgaggg tagagacgtt gaagcatatt tcgtgaggca gggggtaagg
       61 gaagggccgt ttcacgcacg agcatcaccc gatcggcttg ctcatcgcgc agcatgtcgc
      121 gcatgcgttt ggtggcggac gcgaccgatt gtccgtgggc gctgttgttg gtttcgaaat
      181 acaacctgag tcgcttcgtc gacagcagca gggtaatgcg acagcctgct cgtgaacgtg
      241 tacgcatggc agggatgtcg acgttttcga tgcgggctcc gaataccgtt ttcccatgca
      301 accaggcggt ggtgagagcc tctcgcaaag cgctttgaac gatctgttca cgcacttcgg
      361 gcattcgttc cggccgcctt cgatcgtctt cgatcatggc atgcagcctg cgttgtattt
      421 cctccgaacg gacgattggc cttggctcgg cgggtttcga atgggttcct cccgcccgat
      481 cgtcgagcca ttgacgcatg aatcgcagga tcgatcgtgg agatcgaagg tgattggatc
      541 gaatcaggtc gagcaaagca tcggattcga aaggataagc gggattggct ggtgcgagtc
      601 cgacaccgcg ccagaagcgg tgcatgcgaa gctcgacgag ggacacggct tgttcgggtc
      661 ggagggcaag aaggggtatg ggggctcgcg cttccatacg gtctcgaacg tggtgaggcg
      721 cgcgttggcg gagtctaggc caggtttggg tttggcaaag catcagcagc accgtcggcc
      781 cgcccaggtt atacagctct ccgagcgcgg tgagccaggc agccacggct tcctctccca
      841 accgttcggt tccttcgact tgatcgaaag cgagcgccaa gggaacgtcg gccaatgccg
      901 ccaagtccac gagtaagcgc aagccgcgcc aggcattttg ctcatcatcg aggatgattc
      961 gtgttccaag acgggtagcg gcttgctcgg aaagggatac gccaccgagc caggcgatga
     1021 ggtcttgtcg caaatcggga tgaggaaagc cggcgatgac tcgaaagagt tcgaggtcca
     1081 ttcccgcgag ggcgtcgagc cgttgcgcgg aaagaacgaa gctacgttcg atatcgcccc
     1141 agcagcaagc ggcgacttcg gcgaatgcgc cgaggaaaaa ggagggacga ttgggaatga
     1201 ctttgtcgag ggtttccacc aggtcaggag gcgtccaggg ggcccagcgc accaatcgtg
     1261 cgattcgtag cagcgcacga agcacgatga ccgccagggg agatcccacc gcggaatcta
     1321 aatgtccgga tacggatagt ggacgagata aatttcggac ggtttcgtga aggaagtggt
     1381 ggaaaatacg gtgaaaggct cgcaaaggtt cgatgggagc caacgcaaaa aattgcccat
     1441 ccgtcgagca aaggctgtcc tcggaagctt gacgaagcca ggccaataga tgggttttcc
     1501 cttctcccgg atcgccggta atgacctgga agaccggctg tcgctcctcg gcacacggac
     1561 ccaccagccc aagcagatgc tggcgaaggg aagcatggat ggtcggaacg tccagagggg
     1621 tccgcaaggc gtgcgcgaga acactatccg aaaatggact aggcgcactc gccagcacgc
     1681 gttccaggtc ctcgatatcc gcggttcgat actccatcat cgaagcacca cgaagtacaa
     1741 caacccacgg ttcggcactt cgatgccatg ctgccgatcg gtcagacgct ccggatcatt
     1801 ggccactttc aggtcgatgc ggaattgctc ttccaacgca agcaaggcat cgtcgaaagc
     1861 ctcgcgggag attccaaggg atattagcga ttcacgcacg cgaggcagag gaaccaaggc
     1921 gccgtgtccc cctttcgtgc ttagctcatg gcaagcgtgg cgaagggcgg attcgaattg
     1981 ttcgagcgtt cggggcaaag ctggttggca aggctgctcg gtgacggcag aggctcccac
     2041 ggtgatcgcg atggcttcga ccaacgcgtg aagatcggcg acttgcttgc gtaaggtatc
     2101 caattgttga agtatggttg cgttttggga gccgtcctct ccgtgcaacg cctgcttgcc
     2161 cgcggcggtg atgttcaaac gcgcggaggc accttttcga gcggggggcg tggataggat
     2221 gagttgccgc tcgatcaatt cggaaacggt ggatgaatcg gctttgagcg aggcctgggt
     2281 cacggggccc cgggccagca gtgttcgcaa cacgcgctct tgttgcggtg tcatgactgc
     2341 ggcttagtac agcttttgtc gagtggatac aggcaatcag ggtttgcctg cgaccctcgg
     2401 gttcgacttt gccttcggga aggaggtcgc aaggagagat ctagccatcc acgccgcaac
     2461 gagcggcttt gcagcgcccg ctttcaggac tgaggcaaag gtgttgctac tctttcgttc
     2521 ttcctgcagc cacaacggct acttctctcc gatagcgagt tttggggtcg tgcgccgcaa
     2581 gcgctcgaag ggtggcggtt tgctcactct tgccacgaga cacaggccca aacggttcgg
     2641 gaacaccgcg aagcgacttc gtttcaagct ttgtgataga gccggtgact aaaccatagt
     2701 gactgaaatt gcggcgtcgc aatatcgacg ctacgattcc catagtagag cgcgactttc
     2761 tcgcgttggg aaagcttcac ttcacgataa attcgctgac gatgggaaat atccagacca
     2821 gagggcacgt cgaagagctt gctatctcga agctggaagc tcatggatgg accgttgtcg
     2881 gaggtgaacg ccggggggtg gatctggacc ttttcgttca attcgacgga atcagcggca
     2941 aggtcttctt ccgcaacgcc ttcaatctat cgcaggggat ggcggagact gccaactcgg
     3001 acccatgctc cgtgtggatc gagctgggcg agcccgtcgg agagcccacg tggtggtctt
     3061 tgtgggacca atataggttt ggtcaaggca gcaaagaaca tgcgttcatc gccaccatca
     3121 tccacgaagc tatcggcata gggtggacga aagtggctgg cggagacgtg gagcctgggc
     3181 aaacctggcg atcgccgagc aagacgctcg ataccatcat ctcccgggcg gcgtgtctgg
     3241 cgccgagccg caacgaggct gatcgacgat acgaaatctg gaagaagcaa cggcacaact
     3301 tcggatctcc accgaaaaag catagcctgg tgctattcct cgtccttttg gcgatgcctg
     3361 tcggcgtcat gtttttcgac tggctcgata acggaaagtt aacgatcgct tcgatcgtcg
     3421 gccccgtcct gttgtcgatc ttgcccttca ttttcctcct cgatctgttg aaaacctcaa
     3481 aggaccatct tcggcgcccc cgtaacctgg tcgccggagt ggacgccgtg gtcaatgcta
     3541 tgcggatcac cggcgtgttc gacgttttca aggtgacgac ggcgaagctc cgccacggcc
     3601 ttccggtggt ggttcgctcc cacggctatc tcgaccgtca tcaccaaccc cacgggagcc
     3661 ccaaatccaa tacgaaaggg tcccctgcaa gagctgttgg agacaaaggg atccatatca
     3721 acgatacccc gcacgtgagg tggatgtttg caacaaggag aattggccca gttgattctg
     3781 caactc
//
LOCUS       DAOSOR010000564         3785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_24295_length_3785_cov_4.310724, whole genome shotgun sequence.
ACCESSION   DAOSOR010000564 DAOSOR010000000
VERSION     DAOSOR010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24295_length_3785_cov_4.310724"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..>3785)
                     /locus_tag="PK710_21685"
     CDS             complement(18..>3785)
                     /locus_tag="PK710_21685"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOT12406.1"
                     /translation="RPADVLGGSAKRGPMVVGIRYMSRPGTPNARNQEAYQVVQVMDL
                     GISAKVSRFGSLVWINRLSDGKPVEGAEVRIRTPGTAASSDFVVRTDAHGMALVDGSK
                     LFPAKGQEGSSLILVKHGDDWAYRNASDIISTWRYDVPSNLYGEMSPYGMVFTERGVY
                     RPGDTVKIKGIFRQPEARGTSTPVGKSTKITVSTPDGDDLLSRDATLSEFGTLALEFK
                     IPATAELGYYSIGANVGGTDAVHAWGSFNVAEYRASEFKVTTDLNRRSYIRGDEVRCS
                     AQGDYLFGAPMSGSELRTTITRGPSWFAVPKTDDFITTDHAFEQDREEGYIDSSQLLA
                     TQGKLDAQGKSEAGTKLELPGMRGTEYVNCEVEVTDLSRQTVASFSHATVHPAAFYLA
                     LQTGNEGFAQSGTEIGPQILAVDPEGRRQSGVAVDIELIRRTWVTARESAGTNGYHSV
                     SRAVDTVVSQCSTRTVATPASCNLKVPTAGYFILRAKAKDARGNPVAASTYLYATGSG
                     ESGWAEHDDMRVELVPDKTSYEVGQTARILVKSPFQNAEALVTVERAGIYSHRRMQLH
                     GSMPTLSIPITADFHPNAYVSVVLLRGRSKAAPAQWNAPDVGAPAFRIGYANLQINPE
                     SKRLNVEVNANKKDYRPGEQVAVDLQIRDRAGKGQRAEVTLYAVDEGVLMLTDYKTPD
                     PIPIFTEPHPLRVGFTEARTDLARLTLSPFASTVGEDKGLDGGGGGAARSDFRQSVYF
                     NPSLITDESGKAKATFKLPDSLTSYRIMAVVVGATDQFGFGQSTVTTSLPLLARPAFP
                     RVLRAGDEIDAGVVLTSKTLPKSAIDVKATVQGLELVGESVRQVELDKGQSREVRFRF
                     RANSVGNAKVRFHVQGGGEQDSVEITKAVRAPTSLESVALYGSTRSATGEMLGDLSAI
                     RTDVGSINVSVASTALVGLAGSVEYLTEYPYECTEQLASRLVPLIPLRELARLYSLPL
                     PKNTDAFITNTVTKLVRRQRYDGGFGLWDESRTSHPWVSAYALWSLYHAKKAGASVPK
                     KTIENGRTYLRRYLDDYETASHGLTTAAFVMDILAEMGVSDPGYMNRLFDQRADLPLF
                     AKAYLAHAMAMGGGDEKGRDELIRDIEGHIRVQGNQALVTENLGDAYAELMDSTTRTN
                     ALVLRALLSVKKNHALAEPMTRGLLGARQQGTWRNTQEAAYALLALDEYRKAQEFEEP
                     DFTATVWLGDEQILQQAMTGRSVKSTETILPASRMVKAGDNTLAFQRVVND"
BASE COUNT          758 a         1086 c         1075 g          866 t
ORIGIN      
        1 tcgtattgca gggtgcatta gtcgttgact acacgctgaa acgccaaggt gttgtctcca
       61 gccttcacca ttcgactagc aggcagaatc gtttccgtgg acttgacgct tcgcccggtc
      121 atggcttgtt gcaagatctg ctcatcgccc aaccatacgg ttgcggtaaa gtccggttct
      181 tcaaattcct gcgcctttcg atactcgtcg agagcgagca gggcgtatgc ggcttcttgt
      241 gtgtttcgcc aagtgccttg ttgcctcgcc ccgagcagac cgcgggtcat gggctctgcc
      301 agcgcatgat tcttcttgac ggaaagaagt gcgcgaagca ccagcgcatt ggttcgcgtc
      361 gtcgagtcca tcaattctgc gtaagcatca ccaaggtttt cggtgaccaa ggcctggttg
      421 ccttgaacgc gaatatggcc ttcgatatcc cttatgagtt cgtcgcgtcc cttttcatct
      481 ccgccaccca tggccatggc gtgagccaaa tacgctttgg caaagagggg aagatcggcg
      541 cgttgatcaa atagccggtt catataacca gggtcggata cccccatctc cgcgaggata
      601 tccatgacga acgcggcggt cgtcagccca tgggaagccg tttcataatc atcgagatat
      661 cgtcgaagat aggttctacc gttttcgatt gtctttttgg gaacggaagc ccccgctttt
      721 ttcgcatgat acaggctcca caaggcatac gcgctcaccc atggatggct ggttctcgat
      781 tcgtcccaca gcccaaatcc cccatcgtag cgctgacgtc gcacgagctt cgtaacggta
      841 ttcgtgatga atgcatccgt gtttttggga aggggaaggg agtagagccg ggccagttct
      901 cgcaagggaa tcagtggcac caaacggctg gcgagttgtt ccgtgcattc atacgggtat
      961 tccgtgagat actccacact gcccgcgaga cctacgagcg ctgtcgatgc cactgacaca
     1021 ttgatgctcc ccacatcagt gcgaatggcg gaaagatctc ccagcatttc tccggtggct
     1081 gaacgcgtgg aaccgtataa cgcaacgctt tcgagcgaag tcggagcgcg caccgccttg
     1141 gtgatttcaa cgctgtcttg ttctcctccg ccctgcacgt gaaagcggac tttggcatta
     1201 cccaccgagt tcgcgcgaaa ccggaagcgc acctcgcgag attgcccctt atccaattcg
     1261 acttggcgca ccgactctcc cacgagttcc aacccttgca cggttgcttt cacgtcaatg
     1321 gccgacttgg gcagtgtttt cgacgtaagt acgactccgg cgtcgatctc gtcgccggct
     1381 cggagaacac gtggaaacgc tggccgcgcg agcaacggaa gactggtggt gacggtcgat
     1441 tgtccaaagc cgaattggtc ggtagcaccc acgacgaccg ccatgatgcg atagctggtt
     1501 aggctgtcgg gtagtttgaa ggttgccttg gcctttcccg attcatccgt gatgagggat
     1561 gggttgaaat aaacactctg ccggaagtcg ctgcgtgcag cgcctccacc tcccccatcg
     1621 agtcccttgt cttctccgac cgtgcttgcg aaggggctca aggtgagacg agccagatcg
     1681 gtgcgtgctt cggtgaatcc gacgcgcaga ggatggggct cggtaaatat ggggatcgga
     1741 tcgggcgttt tgtagtcagt cagcatgaga acgccttcat cgacggcata caaagtgacc
     1801 tctgcgcgtt ggccttttcc tgcgcgatca cggatctgaa ggtccacggc tacctgttcg
     1861 cccggtcgat aatccttttt gtttgcgttc acctcgacgt tcaagcgctt ggattcaggg
     1921 ttgatttgaa gatttgcgta accgatgcgg aacgctggag cgccgacgtc tggagcgttc
     1981 cattgggcag gagcggcttt cgaacgcccc cgcagcagca cgaccgatac gtacgcgttg
     2041 ggatggaagt cggcggtgat ggggatcgac agcgttggca tcgatccgtg caactgcatt
     2101 cgtcgatgag aataaatccc cgcacgctcc acggtgacga gcgcttcggc gttttggaaa
     2161 ggagatttga ccaggatgcg tgccgtttgt cccacctcat aagacgtttt gtcaggaacc
     2221 agttccaccc gcatatcatc gtgttctgcc caaccggact caccgcttcc cgtagcgtat
     2281 agatacgtgg aagctgccac gggattgcct cgagcgtctt tggctttggc ccggagaatg
     2341 aaatacccag cggtggggac cttcaggttg cagctcgcag gcgtggcgac cgtccgcgta
     2401 gagcactggc tcactacggt atccacggcg cggctgacgc tatggtagcc gtttgtcccc
     2461 gcgctctctc tcgcggtcac ccacgtgcgt cgaatgagtt cgatgtccac agcgacgccc
     2521 gactgtcgac gaccctcggg atcgacagcg aggatttgcg gaccgatctc cgtgccactt
     2581 tgcgcgaagc cctcgttgcc cgtctgcaaa gccaggtaaa aggcagcggg atgaacggtc
     2641 gcgtgcgaga aagaagcgac agtttgacgt gataaatccg ttacttccac ttcgcagttc
     2701 acatactcgg tgcctcgcat tccgggcaat tcgagcttcg tgccagcttc gcttttccct
     2761 tgggcgtcga gcttgccttg ggttgccaat agctgtgatg aatcgatgta accttcttcg
     2821 cgatcttgtt cgaaagcgtg atcggtcgtg ataaaatcat ccgtttttgg aacagcgaac
     2881 caggaagggc cgcgggtaat cgtggtgcga agctcggaac cgctcattgg cgccccgaat
     2941 aaataatccc cttgtgccga gcagcgcacc tcgtcgccgc ggatatacga tcgacgattc
     3001 agatcggtgg tgactttgaa ttctgacgcg cggtactccg cgacgttgaa cgaaccccag
     3061 gcgtggacgg cgtctgtacc tccgacgttg gctccgatgc tgtagtagcc cagttctgcc
     3121 gtcgcgggga ttttgaactc aagggcaagc gtaccgaatt ccgaaagagt ggcatcgcga
     3181 gacaaaaggt catcgccgtc gggagtggaa accgtgattt tggtgctttt ccccacgggc
     3241 gtggaggtcc ccctggcctc gggctgccgg aaaataccct tgattttgac ggtatctccc
     3301 ggtcgataca cccctcgttc cgtaaatacc atcccgtaag gcgacatctc cccgtacaag
     3361 ttgctcggaa catcgtatcg ccaggtggag atgatatcgc ttgcattacg gtaggcccaa
     3421 tcatccccat gcttgaccag aatcagcgaa gacccttctt gaccttttgc cgggaacaac
     3481 ttcgaaccat ccacgagagc cataccgtga gcgtccgtgc gcaccacgaa atcgctcgat
     3541 gccgcggttc ccggggtgcg aatgcggact tccgcacctt ccacgggttt gccgtcggaa
     3601 agacggttga tccagaccaa ggagccaaat cgagatactt tggcgctgat gcccagatcc
     3661 atgacctgca ccacctgata ggcttcttga ttgcgggcgt taggcgtgcc tggccgtgac
     3721 atatagcgta ttcccacgac catgggaccg cgtttcgcgc tgccaccgag aacgtctgcg
     3781 ggccg
//
LOCUS       DAOSOR010000565         3784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_24300_length_3784_cov_3.369268, whole genome shotgun sequence.
ACCESSION   DAOSOR010000565 DAOSOR010000000
VERSION     DAOSOR010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24300_length_3784_cov_3.369268"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="PK710_21690"
     CDS             complement(<1..1210)
                     /locus_tag="PK710_21690"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12407.1"
                     /translation="MDTPRALPDLAQLVVDTGLVRSEQVQEVLEATTDAKRLAPTLVQ
                     RGLIAGPKLAQMLSYQLCLPWVALEHVRFSPKLLRLIPVELAAKYGVIPVHVRNRTSD
                     GRMTLYVATDDPTNQEALEACSRAARLPVRMMVATTTDVSKALAEQYGEGEFDPSHDA
                     LADTAPDGTALDVPSISRPAPDRDIAVAAARMSSPDLGHVQQLATPAAMNPTPNVVVN
                     PPPRLGPSASSPPPPRVAASTSNTKEPSAAAPVAVTGVPRIVGAPSPTDGPHDDDLEE
                     LDALEEYAHDTDLSHVVPATASAHDDEVEKAKPQSDSSALFDASSWEPPPGAPIDVGP
                     IPLPPPPSFLLDDGLASAPPPAGLMAPPPPPPPAPLIGDSIPPEDLDVELNEEDIEEE
                     RLLAIVLVLDA"
     gene            1430..2206
                     /locus_tag="PK710_21695"
     CDS             1430..2206
                     /locus_tag="PK710_21695"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOT12408.1"
                     /translation="MIKPPPLSALRVLEGEEPRDSLRAIRSMVSEDDLSVVFQPIVDL
                     ATGRLFASEALVRCRIAQFQPPPKLFEHAQQLGCVGRLGRLIREIAIPLCSGAPIFVN
                     VHPGELDQGWLVRPDDPIFSHDADVYLEITESVPMSHFSLCVSVLREVCSRGQVYLVI
                     DDLGAGYSNLKRIADLQPNMVKIDGSLVRGLDANNRQQILVKRVVRLCTDLMASVVAE
                     GIESASELQAVRDAGVQFGQGFLLARPAYPIPALSLPPWG"
     gene            complement(2287..3567)
                     /gene="purD"
                     /locus_tag="PK710_21700"
     CDS             complement(2287..3567)
                     /gene="purD"
                     /locus_tag="PK710_21700"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOT12409.1"
                     /translation="MASRNVLVVGGGAREHALCAALDRSPSVSRVMAVPGNAGIGRKW
                     SCHPIRYGDRDTFFEIVEQNAVDLVVVGPEGPLVEGMADALVKRGIAVFGPTAKAARL
                     EGSKVFMKTFAQEVGIPTAPFKVFKSPTAARAYVRTLDVPPVIKADGLCAGKGVVVAE
                     SVSEALSAVDAILERRAFGESGAAIVVEERLHGQEVSVQAMCDGERMFVLPVAQDHKR
                     LKDGDLGANTGGMGAYAPVPLVTPELEKRIRDRILEPTLTGMRDRGMPFRGVLFAGLM
                     ITPEGDPFLLEFNARFGDPEAQVTLPLLQGDFGQVCYACARGELEEGMLTIENRHSLG
                     VVLASHGYPKNVRTGDSIRGLDEVESLEDVKLFHAGTRALGDRLVTSSGRVVTIVGMA
                     DSLQNARDRAYQACDMISFDGMQLRRDIGGRALS"
     gene            3561..3779
                     /locus_tag="PK710_21705"
     CDS             3561..3779
                     /locus_tag="PK710_21705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12410.1"
                     /translation="MPWPRNITTAAPRLAQQNVVAKDEIRPTIPLLMKGTPPIAPAYG
                     FLPTVHIPFLFSTLSLSYPGLAIGSPLT"
BASE COUNT          796 a         1160 c         1027 g          801 t
ORIGIN      
        1 ccgcgtcaag aaccaggacg atcgcgagaa ggcgctcctc ctcgatatct tcttcgttca
       61 actccacatc aaggtcttcc ggtggaatcg agtcaccgat gaggggtgca ggcggaggag
      121 gaggcggggg agccatcagc ccggcaggag gcggggccga cgccaaacca tcgtccaaga
      181 gaaaagacgg aggaggagga agtggtatcg gtcccacgtc gataggcgct cctgggggag
      241 gctcccaaga agaggcatcg aagagggcgg aagaatcgga ctggggcttg gctttctcca
      301 cctcgtcatc gtgtgcagat gcggtggctg gaacgacatg cgatagatcg gtgtcgtgag
      361 catattcttc taacgcatcc aactcctcga gatcgtcatc atgaggacca tctgtcggtg
      421 atggggctcc aacaatccgt ggaacgcccg tgacagcaac gggagcggcg gccgatggtt
      481 cttttgtgtt cgacgtcgaa gccgcaacgc gaggcggagg tggactcgat gcgctcgggc
      541 ccaatcgtgg tggcgggttg accacgacat tcggagtggg attcatggcc gctggcgttg
      601 cgagttgttg aacgtgtccg agatccggcg aggacattct tgcggcagcc accgcgatat
      661 cccgatccgg cgccggacga gatatgctcg gcacatcgag agcggttcca tctggcgctg
      721 tatccgcgag cgcatcatgg gaagggtcaa actcgccttc gccgtactgc tcagccaacg
      781 ccttcgacac atcggtggtg gtggccacca tcatgcgcac gggcagcctt gccgcacgcg
      841 aacaagcttc caaagcttct tgattcgtgg ggtcatccgt ggcgacgtac agcgtcattc
      901 gtccatcgga agtccgattg cgaacatgca cgggaatgac cccatatttt gcggccaatt
      961 ccacggggat gagacgcaga agtttgggag aaaaacgtac gtgttctagg gcaacccacg
     1021 gcaaacacaa ttgataggac aacatctggg caagttttgg cccagcaatc aaaccgcgct
     1081 gaacgagcgt gggcgccaag cgctttgcgt ccgtcgtggc ttcgagaact tcttgcactt
     1141 gctcacttcg aaccaagccg gtgtcgacca ccaattgagc aagatcaggt aatgcgcggg
     1201 gggtatccaa caacgtcctc gatgttacgc caccctttcg agtacatcgg ggtcatgcct
     1261 acgtcaaccc acctgaaagc aatgctctga taactggatt tcttcataat cccgcctttg
     1321 cattcatgac actcttccct ccattccaga tttttgatat tctatcgata agcacctcag
     1381 acgattagcc cacggcattc tgtgtcgagc agcagcgaaa ggcatgcgcg tgataaaacc
     1441 accacccctc tccgctttgc gagtcctcga aggcgaagag ccgcgcgatt cccttcgcgc
     1501 cattcgttcg atggtttcag aggatgactt atccgtcgtc tttcaaccca tcgtcgatct
     1561 tgccacaggg cgtctttttg cgagcgaggc tctcgttcgt tgtcgcattg cgcagttcca
     1621 acctccgccc aagctattcg agcacgctca acaattgggt tgcgtggggc gcctaggacg
     1681 gctcatccgc gaaatcgcga ttccgttatg cagcggcgcc ccgatttttg tcaatgttca
     1741 tcccggtgag ttggaccaag gatggctcgt acgtcccgat gatccgatct tttctcacga
     1801 tgccgacgtt tacctcgaaa tcaccgaatc cgtgcccatg tctcatttca gcttatgcgt
     1861 tagcgtgctg cgggaagtct gcagccgagg gcaggtgtat ctggtcatcg acgatctcgg
     1921 cgcgggctac tcaaacttga aaagaatcgc tgacttacag ccgaacatgg tcaaaatcga
     1981 cggcagcctc gtacgcgggc tcgatgccaa caaccgacaa caaatccttg tcaaacgcgt
     2041 cgttcggttg tgtacggatc tgatggcttc cgtggtggcc gaaggcatcg agtcggcctc
     2101 ggaactccaa gcggttcggg atgcaggagt ccaatttggg cagggttttc tccttgcccg
     2161 gccggcgtat cccattcctg ctttatccct tccgccgtgg ggctaggctc gtttccttgc
     2221 tttccttcga aggggcggcg atagcgtcgg tgctgtcggc ttgcgtattc gtttaccgac
     2281 gattcatcag gacaaggcac gaccaccgat atcgcgacgc aactgcattc cgtcgaagga
     2341 gatcatgtca caggcttgat aggctcggtc ccgtgcattt tgcagtgaat cagccatgcc
     2401 cacgatggtg acgacccttc cactgctggt cacgagccga tcgccaaggg ctcgggtgcc
     2461 ggcatgaaag agtttgacat cctcgaggga ctcgacctca tcgaggcctc ggatgctatc
     2521 tcccgtgcgc acgtttttcg ggtagccatg agaagcgagc accaccccga gcgagtgacg
     2581 gttttcgatg gtgagcatcc cttcctcgag ttctccccgc gcgcaagcgt aacacacttg
     2641 cccaaaatca ccctgaagca agggcaacgt tacttgcgcc tcggggtctc caaagcgggc
     2701 attgaattcg agtaagaaag gatcgccttc gggtgtgatc atcagccccg cgaaaagcac
     2761 gccccgaaat ggcatacccc gatcacgcat tcccgtaagg gttggttcga gaatgcggtc
     2821 gcgaatgcgt ttttccaact cgggagtcac caacggcacc ggcgcatacg ctcccatacc
     2881 tcccgtgttt gcgccgaggt cgccgtcttt cagcctcttg tggtcctgcg ccacgggcaa
     2941 cacaaacatc ctttcgccat cgcacatcgc ctgcacgctc acctcttggc cgtgaaggcg
     3001 ttcttccacg acaatcgccg caccggactc tccaaaagca cgtcgttcca agatggcatc
     3061 gaccgcggac agggcttctg acaccgattc ggcgaccacc actccttttc ccgcgcacag
     3121 gccatcagct ttgatcacgg gcggcacatc gagcgttcgc acgtaggcac gggccgccgt
     3181 tgggcttttg aatactttga aaggcgccgt gggaataccc acttcttgcg caaaggtctt
     3241 catgaaaacc ttggagcctt ccaagcgggc cgccttggcc gtaggtccaa aaaccgcaat
     3301 cccacgcttg accaacgcat cggccatccc ctcgacaagc ggtccttcag ggcccaccac
     3361 caccaagtcc accgcgttct gttccacaat ctcaaaaaac gtgtctcgat caccataccg
     3421 aatcggatgg cacgaccact tacgcccaat ccccgcattg cccggtaccg ccatgacgcg
     3481 gctcaccgaa ggagatctgt ccaaggcagc gcaaagagca tgctcccgcg ctcccccacc
     3541 tacgaccaga acatttcgcg atgccatggc ctcgaaatat cacgaccgcc gcgccgcgtc
     3601 tcgcacaaca gaacgtcgtt gccaaagatg aaatccgtcc cacgattcca ctgctcatga
     3661 agggaacgcc accgatagct cccgcttacg gatttctacc caccgtccat attcccttcc
     3721 tattttccac actgtccctg tcgtaccctg gactcgccat cggctcgccg ttgacgtagc
     3781 aggt
//
LOCUS       DAOSOR010000566         3772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_24399_length_3772_cov_3.646220, whole genome shotgun sequence.
ACCESSION   DAOSOR010000566 DAOSOR010000000
VERSION     DAOSOR010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24399_length_3772_cov_3.646220"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PK710_21710"
     CDS             <1..753
                     /locus_tag="PK710_21710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12411.1"
                     /translation="AVVARFERPTGVFSRSYAEAVLEHYLSEAARIMQVNVQALLIGV
                     LRVTIDETSDKTSPKHKATASASVPTFETFFKPQAFESNGSVFFERICPGWAEKPVVD
                     RDNGTLTGTLNFSESGLDPVVWSRIRGCRFRLEGVKILLGDDAHDHVRLWLGHGVTFA
                     SFGQHPILFDVDLSAEVAGLQAPFELSFRFDPEKGTLAIVIDIPSDPPLGSVIVSSNA
                     SAELTVEAGNGTFQCNLSSRECTSDSADGFTF"
     gene            696..1562
                     /locus_tag="PK710_21715"
     CDS             696..1562
                     /locus_tag="PK710_21715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12412.1"
                     /translation="MQPEFTRMHVGQRRRLHVLIVSFWLIGVLASPVGAEPRHDANFR
                     VTEDTADTLPKQAVRVGLFKWQYGVFDSLMVGTYTVPWVVALANFQLKYRYFDDPHWS
                     AAIEMGVGRLDVTRLRPFETHPGDAIIVVSSVEPSLSYRPHDRVVLSAAIPMSKISVD
                     GTVNTKAFEGVLEGAVDNLQLTATMGWKVSSELSLWIHGRYLALQHIYGDTEATYHPD
                     PYTRIVIHADARTTWLSLGDTWSVLPQAALSFGSFHVRAGIGYGNWNISPVNFVLPQK
                     GLIPQLDAFFLW"
     gene            2131..>3772
                     /locus_tag="PK710_21720"
     CDS             2131..>3772
                     /locus_tag="PK710_21720"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT12413.1"
                     /translation="MADATADFKKGSLGIKFVLLAVGIAALAIVLVYVGIKREGEKLT
                     IEQVVNEQKRIFKLQRNDQIAEWRKWASSTHEFELQQQALLQLAWLNDKDGVPIAIQA
                     LQQPDHRIRGVAAQVVAHFGSPTADAAKPAVNKALLESDASDEPQLAWALVELNDKTA
                     LDRVLKVYRAGHLSKVQRVGGGSAFNPEKLATMASPEQWASYVKDESPSVRQLVATIL
                     SSNADPRFTDPLITLVQDSDVDVAREAAPGLGRIGDEKARKPLLDALGKADKDSRKKF
                     LEALRDGIGGDGLVMALAAVDKSKPETMWHQTRVIFNLLKELADPRAADALVTYIESG
                     PQRHWKTEAAMRLAEVGDPRAVPYLAERMRLNTVKLYGDSELVEEKALARGDEERIVG
                     ARMLADLAKLHPDKVDTFREQAEEAVMFWISDMPQPHANALRFLAQVKSEKTRTKLRD
                     WSFPKASLPREGQQPPMPDEWVVAQSALRYLGVMKDDASWGKFENQLKRRRADANDID
                     ATMQSLMGGGLAILGMSLRALGVGVSDGFAEWGDTRAYK"
BASE COUNT          777 a          963 c         1200 g          832 t
ORIGIN      
        1 gcggtggtgg cgcgtttcga aaggcccact ggggttttca gtcgttcgta tgcggaagca
       61 gttctcgaac actacctgtc ggaggccgca cggatcatgc aagtcaatgt ccaggcgctg
      121 ctcatcggtg tccttcgggt cacgatcgac gaaacgagcg acaagacgtc acccaaacac
      181 aaggcgacag catcggcgtc tgtcccaacg ttcgaaacgt ttttcaaacc ccaagccttc
      241 gagagcaatg gcagcgtatt tttcgaacga atttgtcctg gctgggcgga aaagcccgtg
      301 gtcgatcggg acaatggcac gttgacgggc accctgaatt tttccgagtc gggactcgat
      361 cctgtcgttt ggagccgcat tcgtggttgt cgattccggc tggaaggagt caaaattctt
      421 ctcggtgacg atgcccacga tcatgtgcgg ctatggctcg ggcacggcgt gacgttcgct
      481 tcgttcggcc agcatcccat cctattcgat gtcgatttat cggccgaggt ggcgggtttg
      541 caagctcctt tcgagcttag ttttcgcttc gatcccgaaa agggaacgct ggcgatcgtg
      601 atcgacatcc cctcggatcc gcccctcgga tcggtcatcg tatcgtccaa cgcttcggcg
      661 gaactaacgg tagaggcagg caatggtact tttcaatgca acctgagttc acgcgaatgc
      721 acgtcggaca gcgccgacgg cttcacgttc taatcgtatc gttttggctc atcggcgtct
      781 tggcttcccc ggtgggtgcg gaaccaagac acgatgccaa ttttcgagtc accgaagaca
      841 cggcggatac gttgcccaaa caagcggtgc gagtcgggtt gttcaagtgg caatatggcg
      901 tattcgattc gctgatggtg ggcacgtata cggtcccctg ggtcgtggcg ctcgccaatt
      961 tccaactgaa atatcgctac ttcgacgatc ctcattggtc cgcggcgatc gagatgggag
     1021 tcggacgcct cgatgtcacg cgtcttcgtc cgttcgaaac gcatccgggc gatgccatca
     1081 ttgtggtgag cagtgtggag cccagcctct cgtatcggcc tcatgaccgc gtggtgctca
     1141 gcgcggccat cccgatgagc aagatttcgg tggatggtac tgtcaacacg aaagccttcg
     1201 aaggggtgct cgagggggcg gtcgacaatc ttcaactgac ggcgacgatg ggatggaaag
     1261 tatcgtcgga gctttcgttg tggatccatg gccgatacct cgcgctgcag catatctacg
     1321 gtgacacgga ggccacgtat catcccgatc cgtatacgcg aatcgtcatc cacgcggatg
     1381 ccaggaccac ctggctcagc cttggggaca cgtggtccgt tctgcctcag gcagcactat
     1441 ctttcggcag ttttcatgtg cgcgcgggca tcggctacgg caattggaac atctcgcccg
     1501 tcaatttcgt gcttcctcaa aaagggctca ttccccagct cgacgcattt ttcttgtggt
     1561 aaggcggagg tttcgaaggc gagtggttcg catgggatcg ggggatgagc gcggtgtggg
     1621 caaagatgga agggggggaa tgcgtggggt gggagcttcc aatgggggga cgtttcgagg
     1681 gggaggggcc gcggagcgag gcggtattat tttctgttta tatataatat acatatatcc
     1741 ttgattttat actagacaga gggatattgg tcggatgttt tgggtagggg gggaggtgaa
     1801 acggggcggg catggggtgg tgaaagcgcg gggaggcgat aaaaagaata ttcgtgtaat
     1861 ttcatatagt tacaacagat tcggaggtcg atttggcctt atgattccgt gtgggctgca
     1921 cgagtcgata ccaatttgtc gaaggctgta ttactcgaag gggccaagct cccacgccaa
     1981 ggtgtggggg agagacaatg aaccccaacc ctaatcggac gcagacgcgg cgcgatcggt
     2041 cgtgattgcc ccttgacgaa aggggtgttg aacggctctg gtatgtcccg cgtgctgcgt
     2101 caaagcagcg gcctactcgt tggagggacc atggcggatg cgactgcgga cttcaagaag
     2161 ggctccctgg gaatcaagtt tgtattgctg gctgtcggca ttgccgcgtt ggccattgta
     2221 cttgtgtacg ttggcatcaa gcgcgaggga gagaagttga ccatcgaaca ggtggtcaac
     2281 gagcagaagc gtattttcaa acttcagcga aacgatcaga tagccgagtg gcgcaagtgg
     2341 gcttcgagca cccacgaatt cgaattgcag cagcaagcgt tattgcagct tgcgtggctc
     2401 aacgacaagg atggcgttcc cattgccatt caagcgttgc agcaacccga tcatcgcatt
     2461 cggggtgttg cggcgcaggt agtggcgcat ttcggttcgc ccacggccga tgcggcaaag
     2521 ccggcggtca acaaggcgtt gctcgagtct gatgcatccg atgagcctca gcttgcctgg
     2581 gcgcttgtcg aactcaatga caagacggcg ttggaccgcg tcttgaaagt ctaccgagcg
     2641 ggtcatttat ccaaggtgca gcgtgtggga ggcggctcgg cgttcaaccc ggaaaaactt
     2701 gccacgatgg cgtcgcccga gcagtgggct tcgtatgtca aagatgagag tccgagtgtt
     2761 cgtcagctcg tggcaaccat cttgtcttcg aatgccgatc cgaggtttac cgatccgctg
     2821 atcacgctcg ttcaggattc ggatgtggat gtggcccggg aggccgcgcc tggattgggg
     2881 cgcataggcg atgagaaggc gcgcaagccg ctgctcgatg cgttgggcaa agcggacaag
     2941 gacagtcgaa agaagtttct cgaagccctt cgcgatggca ttggcggcga cgggctggtg
     3001 atggctcttg ccgcggtgga caagtcgaag ccggaaacga tgtggcatca aacgcgagtc
     3061 attttcaact tgctcaagga gttggcggat cccagggccg cggacgcgtt ggtgacgtac
     3121 atcgaatcgg gtcctcagcg acattggaaa accgaagcgg cgatgcgtct ggcggaggtg
     3181 ggggaccctc gtgcggtgcc gtacttggct gaacgcatgc gtctcaacac cgtcaagctc
     3241 tacggtgatt ccgagttggt cgaggaaaaa gcgctcgcgc gtggcgatga agaacgcatc
     3301 gtgggcgcgc gtatgcttgc ggacctcgcc aagcttcacc cggacaaggt cgatactttc
     3361 cgtgagcaag ccgaggaggc cgtgatgttc tggatctcgg acatgccgca gcctcacgcc
     3421 aacgcccttc gttttctcgc tcaggtcaaa tcggaaaaaa cgcgcaccaa gctcagggat
     3481 tggtctttcc cgaaagcatc gctgccgcgg gaaggacagc agcctcccat gcctgatgaa
     3541 tgggtcgtgg cacaaagcgc gttgcgttac ctgggcgtca tgaaagacga tgcttcttgg
     3601 ggcaaattcg aaaaccaact caagcgacgt cgcgcggatg ccaacgatat cgatgcgacc
     3661 atgcaatcgc tgatgggcgg tgggctggcc attctgggca tgtctttgcg agcgttggga
     3721 gtcggtgttt ccgatggttt tgccgaatgg ggcgataccc gggcgtacaa gc
//
LOCUS       DAOSOR010000567         3764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_24468_length_3764_cov_4.107846, whole genome shotgun sequence.
ACCESSION   DAOSOR010000567 DAOSOR010000000
VERSION     DAOSOR010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24468_length_3764_cov_4.107846"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..419
                     /locus_tag="PK710_21725"
     CDS             54..419
                     /locus_tag="PK710_21725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12414.1"
                     /translation="MSSKVLFVIFSDEPCRRNHAIRYALDLHHKGHVARIILEGAATG
                     SMRELGDRGSDFSKLFWEAKDLGLVAGACRAASAGCGDKNHSVLSIAQSNGIELLDAA
                     DGHASLEPFIRDGFQVVVF"
     gene            474..>3764
                     /locus_tag="PK710_21730"
     CDS             474..>3764
                     /locus_tag="PK710_21730"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT12415.1"
                     /translation="MTSAPQISTETRSSPPRARSSSASSYPLFNPTRIARSLSVEPGE
                     ERRTALLFVLLILASIVFDMGRATRDALFLTRYPVTWIAHMWIAYGVVSSVAAVGYGW
                     ISSRVSRARLAIIFAIFSAVSYVAFRVAIGADFGWAIVVFYVWAEVIANLFILQAWAI
                     TNDLHDPRSGRRLFGLIGAGRIVGMLFSGFLTGSIVRAIGTPNLIVVVAAIMIVFAGL
                     VRFIGTRFPLARPKQQPGAGREPTASLRRHKKYTFFLSIMLLTAFIALTVGDYQFKAI
                     VKFSYPERDDVAEYMARFYALMGTLALFVQLFVAPRVLKYLGVIPALFAMPVAFLGST
                     AALLFSPVLPIATMMKLSDNGLQYTIHEATMQLLYFAYPASLRTRVRAILDAMIKPLG
                     CSLGGVVLVFLSPVPFAGESLTSLAARVASVGFVSLTLGIAWVGVLPLVRWAYVDALH
                     RALVRRQSDLREEVELQLDASMEKVLRTTVHSGTPVQVTFAFEVLALSSPKAAREEFP
                     RLIAHESPIVRAFGVEQIARLDPERAADLARAQLADPDPDVRCAAIATLGSVRQEDAV
                     DEIEHFAYDDAHPRLRNEAIVTLISHGGLPGVLAGGARLQQLIHSRDENDRIEAAVVV
                     GNVGQKGLARTLKPLLKDESLLVRRTAATAARGCASTGLLDAMLEAMSERSLVKPLIA
                     AIIAVGPEAIPELANSLRNEDTPRLVRLSIPRVLHGIGTMEALEVLRGCFGTDDEGVR
                     QKCLASASRLREELQAPALPPQTLKPWIQREIEDHVTARDGWLSVRPWLTRPLLDRQL
                     QLELRGHIVRIMRLCEQAYPREQVAAARNGIFSRDATRRANALEVLDNVLDREHRLAI
                     VDIVGRFATEYPFVDDREPVTTPVPERVKHWFAQRVALPGFYRRSVLFEAVGHNRVCD
                     LAEAALAHTRRSNPFLRENALIALAACRPVGWQDRMERALNDEDAVVRAYATYVMQTG
                     RSGLDPGDEMYTTVEKILYLQGVSLFSNVPGNELVPLAMRARVIRLAPGDVVFHEGDP
                     GDSLFIVFHGQIALHAGGRELAVLGEGEVLGELAMLDEAPRTVTATAVGDADVLQVST
                     EDFRVAVQD"
BASE COUNT          691 a          987 c         1136 g          950 t
ORIGIN      
        1 tcatgtcgtt gcgcttccgg tcatggtaac gtgccgaccg gaaaggaaat gccatgtcgt
       61 cgaaagtgtt attcgtcatc ttttccgatg aaccttgtcg tcgcaatcat gcgattcggt
      121 atgcgttgga tcttcatcac aaaggacatg tggcgcgaat tatcttggaa ggtgcggcca
      181 cgggcagcat gcgcgagctg ggtgatcgtg gctccgactt ttcgaagctg ttttgggaag
      241 cgaaggattt gggtctcgta gcaggagcgt gtcgagccgc gtcggctggt tgtggagata
      301 aaaaccacag cgtgttgtcc atcgcccaat cgaatggaat cgaattgttg gacgcggcgg
      361 atgggcatgc cagtctcgag ccattcatcc gcgacggatt tcaggtagtc gtgttctgac
      421 gacgtggttt ctgtcgtatt gatctatccg aacatggatg gcaggcgatt tcggtgacga
      481 gcgcacctca gatttccacg gagactcgat ccagtccgcc tagggcgcgc tcttcttcgg
      541 catcatccta tccattattc aatcccaccc gtattgctcg ttctctttcg gtcgaacctg
      601 gtgaagagcg gcgaaccgcg ttgctattcg tgttgttgat cttggcctcc atcgttttcg
      661 acatggggcg agcaactcga gatgcgcttt ttttgacgcg ctatcccgtg acgtggattg
      721 cccatatgtg gattgcgtac ggagtggtgt cttccgtggc agccgtgggg tacgggtgga
      781 tttcctcgcg cgtttctcgt gcgcgcctcg ccatcatttt cgccatcttc tccgctgtgt
      841 cctatgtcgc gttccgcgtg gcgattggtg cggacttcgg ttgggccatc gttgtttttt
      901 atgtctgggc cgaggtcatt gcgaatctgt tcatccttca agcctgggcg attaccaacg
      961 acctgcacga ccctcgaagt ggccgaaggc tatttggtct cattggcgcg ggacgaatcg
     1021 tggggatgct ttttagtggt tttcttacgg gcagtatcgt tcgcgctatt gggacgccca
     1081 atctcatcgt ggttgtcgcc gcgatcatga ttgttttcgc aggtcttgtg cgattcattg
     1141 gaacacgttt cccactggca agaccgaagc agcagccggg agcgggacgg gagccgacag
     1201 cttcgttacg acggcacaaa aaatacacct tctttctgtc gatcatgctc ctcacggcgt
     1261 tcatcgcgct gacggtcggc gattatcagt tcaaggcgat cgtcaagttc tcatatcccg
     1321 aacgggatga tgtggccgag tacatggctc gattctacgc tttgatggga acgctcgctc
     1381 tatttgtcca actcttcgtt gcaccgcgag tgctcaagta tcttggtgtg attcctgcgt
     1441 tatttgccat gcctgtcgcc tttctcggaa gcaccgcggc ccttctgttt tctccggttc
     1501 ttcccatagc gacgatgatg aagctgagtg acaacggatt gcagtacacg atccacgagg
     1561 ccacgatgca gcttctgtac ttcgcgtatc cagcctcgct tcgaacgcgt gttcgcgcaa
     1621 ttttggatgc gatgatcaaa cctttgggct gttctttggg gggcgtcgtg ctggttttct
     1681 tgtcgcccgt gcccttcgcg ggagagtcat tgacgagctt ggctgcgcgc gtggcttccg
     1741 tgggcttcgt gtccttgacg cttggcatcg cctgggttgg ggttcttccc ctggtgaggt
     1801 gggcgtatgt tgatgctctt catcgagctt tggtgcgtag gcagtcggat ttgcgagagg
     1861 aagtggagct tcaactcgat gcctcgatgg aaaaagtgct tcgaactacc gtgcattcgg
     1921 gcactcccgt gcaagttacg ttcgctttcg aagtgctggc attgagttcg cccaaggctg
     1981 cgcgcgagga gtttccacga ctcattgctc atgaaagccc aatcgtccgt gcatttgggg
     2041 ttgaacaaat tgctcgtctg gaccctgaac gagcagccga tttggcacgg gcacagctcg
     2101 cggatccgga tccagacgtt cgctgtgcgg cgatcgcgac attgggttcc gtgcggcagg
     2161 aagatgcggt cgatgagatc gagcactttg cgtatgacga tgcgcatcca cgacttcgca
     2221 acgaagccat tgtgacgctc atttctcatg ggggtttgcc aggggtgctg gccgggggcg
     2281 cgagattgca acaattgatt catagccgtg acgaaaacga ccgaatcgag gcggctgtcg
     2341 ttgtcggcaa tgtgggacaa aaggggcttg ctcgaaccct caaaccactg ttgaaagacg
     2401 aatcgctgtt ggttcggagg accgccgcaa cggccgctcg cggctgcgct agcacggggt
     2461 tgctcgatgc gatgctcgag gccatgtcgg aacgctccct cgtcaagccg ctcattgcgg
     2521 ccatcattgc tgtcgggccg gaggcgatac cggaattggc caatagcctt cgaaacgaag
     2581 atacgccgcg tctcgtgcga ctcagtatcc cgcgtgttct tcatggaatc ggaacgatgg
     2641 aggctctcga agtgcttcgc ggctgttttg gcacagacga tgagggggtg cgtcagaaat
     2701 gtctcgcgag cgcctctcgg ttgcgtgaag aactccaggc gcccgctctt cccccacaaa
     2761 ccctgaaacc ctggatccaa cgagagatcg aagaccacgt taccgcgcgg gatggatggc
     2821 tttctgttcg tccttggctt actcgtccgc tgttggaccg acagctccaa ctcgaactac
     2881 gcggacacat cgttcgcatc atgagacttt gtgaacaggc atacccacgc gaacaagtgg
     2941 cggctgctcg aaatggcatt ttttctcgtg atgcaacccg aagagccaac gcattggaag
     3001 tgctcgacaa cgtactggac cgagaacatc gtcttgcgat cgtggacatc gttggcagat
     3061 tcgcgacgga gtaccctttc gtagacgacc gtgagcccgt taccaccccc gttcccgaac
     3121 gtgtcaagca ttggtttgcg cagcgtgttg ctctgcccgg cttctatcga cgctccgtac
     3181 ttttcgaagc ggtcggtcat aatcgcgttt gtgatcttgc cgaggcggcc ctcgcccata
     3241 ctcgtcggtc caacccgttt ctaagagaaa acgcgctcat cgcgcttgcg gcgtgccgtc
     3301 ctgtcggatg gcaggaccgt atggagcgtg cgttgaacga tgaagatgcc gtggttagag
     3361 cgtacgctac ttacgtgatg caaacaggtc gttcgggcct ggatccgggg gacgagatgt
     3421 atacgactgt agaaaaaata ttgtatttac aaggggtttc gctgttttcg aacgtgccgg
     3481 gcaacgagtt ggtgcccctg gctatgaggg ctcgagtcat tcgactggca cccggtgatg
     3541 tggtcttcca cgagggcgat cctggagact ccttgttcat cgtgtttcac ggtcagatcg
     3601 ccttgcatgc gggagggcga gagctggccg tgttggggga aggagaagta ttgggtgaac
     3661 ttgcgatgct cgacgaagcg ccgcgtacgg tgacggctac cgcggtgggt gatgcggatg
     3721 tgttgcaggt atcgacggag gactttcgcg tggccgtgca agat
//
LOCUS       DAOSOR010000568         3759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_24498_length_3759_cov_4.526188, whole genome shotgun sequence.
ACCESSION   DAOSOR010000568 DAOSOR010000000
VERSION     DAOSOR010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24498_length_3759_cov_4.526188"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PK710_21735"
     CDS             <1..515
                     /locus_tag="PK710_21735"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOT12416.1"
                     /translation="ITWPDINKISWDIVALYASAAAIGTGLAVTGASSWLAVTIVQQL
                     PPWATSGEGLAMVSSLLTGLVTNFMSDDAAVATLGPILVPMAQVSETSPWMVGFACAF
                     ASSFANVLIIGTPNNVIAYSLAKNPDTGEQLVTLKDFAKHGLIITVLSLLVLWGWALF
                     GYWRWIGFSS"
     gene            557..1108
                     /locus_tag="PK710_21740"
     CDS             557..1108
                     /locus_tag="PK710_21740"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOT12417.1"
                     /translation="MDTRPVKELMLPLAHYATTSSNSTLRDALLALSKAQLGLTNDRH
                     HHRAVLVLDDQDNVIGKLTHWSILKSLQPSVFSESDRVNLARAGLSLDFIADLVERHP
                     YQGPMERMCALASRIHVRDAMVPVQESIDEQARLSEAIVEMVRLHIQSILVTRHGKVV
                     GILRMSDVFEEVADVIRHRPAVE"
     gene            1105..2418
                     /locus_tag="PK710_21745"
     CDS             1105..2418
                     /locus_tag="PK710_21745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOT12418.1"
                     /translation="MNTMVDPGKTRELGWVGKSGWAGAGTTLIAMNLLVLIIGWCCPN
                     DILQRHVVGLILSMLAAAFVYGFCRRTERAEQAQSQAIVESTDRTKAQRSLLKQTYRA
                     FFASATDGFIVLDDDDVILEANPAACRMHGWQVGTLAGVNVRELIGPNSLHQYRVFKE
                     RDEAAGVFRADAEHLRKDGTTIEIEVRASRLRLGNERRMIAVFTDVTERNRAGQRLGM
                     LSRKALMAQEEERARVSRDLHDELGQLLTASRFELGWLEKHTSQMPQEASIALQRAVE
                     AVEQSAHELRRICRGLRPPLLDDLGLEPAVRLLTQDHEDRTGMRVDLESHVDESVAMS
                     NEISLCTYRILQESLNNISRHAGATSVDVTLVCGETELMLSVYDNGKGFDVEALAAAS
                     GVGITGMRERASLVGGLVEIRSAPHHGTRVVFRVPLTTRSGEETP"
     gene            2415..3053
                     /locus_tag="PK710_21750"
     CDS             2415..3053
                     /locus_tag="PK710_21750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT12419.1"
                     /translation="MIRILVVDDHTMVRAGLCRLLDNEPDIQVVDQSGSGREAVQLCR
                     EHKPDVVVLDYSLPDLDGLETTRQIVALELGIRVLILTMYANEEYATRVIRAGASGFI
                     VKVASTDELLTAIRKVAQKGVYVSPSIMEKMVGRMSQVKGEVPEAVLSDREMQVLIQL
                     ARGSTSREVSKALSLSLSTIETYRSRILEKLDLRNNSDMTRFAIRRGLIDIE"
     gene            complement(3181..3717)
                     /locus_tag="PK710_21755"
     CDS             complement(3181..3717)
                     /locus_tag="PK710_21755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12420.1"
                     /translation="MRWGLAKIEPEGERELRIELPLALVVQRLRAQIDALHAWQGGGI
                     TFGRKPFLGEVRGSSFSIRASSSRRDGATVFLVGKLHGCDRGTLIRYRLETPDAYLRS
                     LPFFGVVPVVLFAVGGYFGSRLVAFEGTASSVFMAGVGAIVGLCASIFLISFARLGVR
                     RDHRRLQRFARFSFLIEP"
BASE COUNT          851 a         1029 c         1087 g          792 t
ORIGIN      
        1 tcatcacatg gcccgacatc aacaaaatct cgtgggatat cgtagcgtta tatgcgagcg
       61 ccgccgccat tggcaccgga ctcgccgtca cgggagccag cagttggctc gctgtgacca
      121 tcgttcaaca gcttccccca tgggcgacct ccggagaagg gctcgccatg gtctcctcgc
      181 tgctgacagg ccttgtgacg aatttcatga gcgatgacgc cgcggtggcc acccttggac
      241 cgatcctggt tccgatggca caggtttcgg aaacgtcccc ctggatggtt ggtttcgcct
      301 gcgccttcgc ctcatcgttc gccaacgtgc tcatcatcgg cacacccaat aacgtcatcg
      361 cctactcgct tgccaaaaat ccggataccg gcgagcagct cgtcacactc aaggatttcg
      421 ccaagcacgg gctcatcatc accgtcctat cgctgctcgt gctgtgggga tgggccctgt
      481 tcggttactg gcggtggata ggtttttcat cgtgacgtcg aaccatctgc ggaaggcaac
      541 cgacaggaga cttttcatgg acacgagacc cgtcaaagag ttgatgcttc cgcttgctca
      601 ctacgccacg acctcgagca atagcacctt gagggatgcc ttgctcgccc tgagcaaagc
      661 ccaactcggg ttgaccaatg atcgtcatca ccatcgcgcg gtgttggtcc tcgatgatca
      721 agacaacgtc atcggcaaac tgacccactg gtccatcctg aaaagccttc agccgagcgt
      781 gttttcggaa agcgatcgag tcaacctcgc gcgagccgga ttgagtctcg atttcatcgc
      841 tgacttggtc gagcgccacc cttatcaagg acccatggag cggatgtgcg ccctggcatc
      901 aagaatccac gtacgagatg ccatggtgcc ggtacaagaa agcatcgatg aacaagcgcg
      961 gttgagcgaa gccatcgtgg aaatggtccg tctccatatt caatccatcc tggtgacgcg
     1021 ccatggcaag gtcgtgggga ttctgcgcat gtcggatgta ttcgaggagg tcgcggacgt
     1081 gattcgtcat cgtccggcgg tcgaatgaac accatggtag atccgggcaa aacacgcgaa
     1141 ctgggatggg tgggcaaaag cggatgggct ggagcaggca ccacactgat cgcgatgaat
     1201 ctgctggtgc tcatcatcgg ttggtgttgc cccaacgata tcctgcagcg ccatgttgtc
     1261 gggctgatct tgtcgatgct tgccgcggcg ttcgtatacg gtttttgtcg acgcaccgag
     1321 cgggcggagc aggcacaatc gcaagccatc gtcgaaagca cggatcgaac caaagcccag
     1381 cgttcgttat tgaagcaaac gtaccgagcg ttttttgcct ctgcgacgga tggattcatc
     1441 gtgcttgacg atgacgatgt aattttggag gccaatcctg ccgcgtgccg catgcatggg
     1501 tggcaggtcg gtacgctggc tggcgttaac gtaagggaat tgattggtcc aaactctttg
     1561 catcagtatc gggtgttcaa ggaacgcgac gaagcggcgg gggtatttcg cgcagatgca
     1621 gagcatcttc gaaaagatgg aacaaccatc gagatcgagg tgcgggcaag tcgtctacgc
     1681 ttgggaaacg aacgtcgaat gatcgccgta ttcacggatg tcaccgaacg caaccgagca
     1741 gggcagcggt tgggaatgct atcgcgcaaa gcattgatgg cacaggaaga agagcgagcg
     1801 cgcgtgtctc gggaccttca tgatgagttg gggcaattgc tgacggccag tcgtttcgag
     1861 ttgggatggt tggagaaaca taccagccaa atgccccaag aagcatcgat cgcgttgcag
     1921 cgcgccgtcg aagccgtgga acaatcagct catgagttgc gaaggatttg ccggggtctt
     1981 cgccccccgc ttctcgacga cttggggctc gaaccggcag ttcgcctatt gacccaagat
     2041 catgaagatc gaacggggat gcgtgtcgat ctggagtccc acgtggacga atccgttgcg
     2101 atgagcaacg agatttctct ttgtacgtac cgcattttgc aagaatcgct gaacaatatc
     2161 agtcggcatg caggagcgac gagcgtggac gtaacgctgg tgtgtggcga aacggagttg
     2221 atgttgtcgg tatacgacaa tggcaaggga tttgatgtag aggctctcgc ggcggccagt
     2281 ggagttggca tcaccggaat gcgcgaacgg gcttccctcg tcggaggatt ggtggagata
     2341 cgctccgctc cccatcatgg tactcgcgtg gtatttcgag ttccgttgac gacacggagc
     2401 ggtgaggaga cgccatgata cggatacttg tcgtggatga tcacaccatg gttcgagcag
     2461 gtctttgtcg tcttctcgac aatgagcctg acattcaagt cgtggaccaa tcgggttcgg
     2521 gacgagaggc tgtacagctt tgtcgcgaac acaagcccga tgtggtcgtg ctcgactaca
     2581 gcttgccgga tttggacggc cttgaaacca cgagacaaat cgttgcgctc gaactcggaa
     2641 tccgcgtact cattctcacg atgtatgcca atgaggaata tgcgacacgg gtcattcgtg
     2701 ctggtgcatc ggggttcatc gtcaaagtgg catcgaccga tgagcttttg acggccatcc
     2761 gcaaggtggc gcaaaagggc gtttatgtaa gtccttccat catggaaaag atggtaggac
     2821 ggatgagcca ggtcaaaggg gaggtcccgg aggccgtatt gtcggaccgc gaaatgcagg
     2881 tgctcattca gcttgcaaga ggttcgacga gccgagaggt atccaaagct ttgagtttgt
     2941 cgttgagcac gatcgaaacg tatcgcagca gaatcctcga gaagctcgat cttcgaaaca
     3001 actcggacat gacccgcttt gccattcgtc gggggctgat cgatatcgag tgatggagtt
     3061 ggttagcctt ggtgaacagg tccaggctgg cagctccgac ggtattcgcc cctcgacttc
     3121 gcccatcaac ctttctgtcg cgccgccatt cggctgatga tccgcttgcc ccaaagccct
     3181 tcacggctcg atgagaaatg aaaaccgggc aaaccgttga agccgtcgat ggtcccgacg
     3241 aacacctaac cgcgcgaaac taattagaaa tatggaagcg cacaatccca cgatcgcccc
     3301 cacccctgcc atgaaaaccg aggaagccgt cccttcgaaa gcgaccagcc tgcttccgaa
     3361 ataacctccg acggcgaaaa gcacgaccgg cacgactccg aaaaacggca gagaacgaag
     3421 ataggcatcg ggcgtctcga gtcgataacg aatgagggtg cctcgatcac agccatgcag
     3481 ttttcccacg agaaaaacgg ttgccccatc tcttcgggaa ctcgaggccc gaatggaaaa
     3541 cgatgagccg cggacctctc caagaaacgg ttttcgtccg aaggtaatgc cccctccctg
     3601 ccacgcgtga agggcatcga tctgtgcgcg caagcgctgc accaccagag ccaaaggaag
     3661 ctcaatccga agttctcgtt ctccctcagg ctcgattttc gccaatcccc aacgcacggt
     3721 cgttcaccat gtcacttacc tggctcgtct tgcaagcat
//
LOCUS       DAOSOR010000569         3752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_24565_length_3752_cov_3.678388, whole genome shotgun sequence.
ACCESSION   DAOSOR010000569 DAOSOR010000000
VERSION     DAOSOR010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24565_length_3752_cov_3.678388"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            355..2661
                     /locus_tag="PK710_21760"
     CDS             355..2661
                     /locus_tag="PK710_21760"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOT12421.1"
                     /translation="MRLFIFFTLSWVVASLPACSCGGEEDSTASPQKGSDGGFKDAAD
                     SGTDAPSDTNLDQFFPFDADECVNGQPCGDGGICAGNTCCSTEKACGNACCGDSQICS
                     FQVCVTPGKDCLDSSECEDNEYCEYGLGEPMEAGVPDSGCSGGHIPLQGKCVPRPPQC
                     SEVDGGSPDGGPLTCLDECKWVPETTDFQAELKYAWGGMTSPPYDSDIMMSPIVIQLD
                     DDDCDGKVTANDIPEIVFNTFSGGAYTGIGTVHAISIVQGQVVEKWSVPGVFNASSEL
                     AGGNIDGEPGNEIVGCGAGAVIALRADGTELWRNASGLACRVPAIANVDGDGTPEVIV
                     EGGILDGATGETKVALPSHPWIAADLDDDGQLDLVGPQLAVHADGTQFVDTGMAGTFA
                     AVGDFDLDGNPEVVAVNTSNHTMWVWRHDPSSANGAVIVRDPIDINGALDPALCPPGS
                     SGATHGGGPPTIADFDGDGIPDVALAGGVGYAILSGAKLMDPAVAGPDTFLWVKQTRD
                     CSSAATGSSIFDFNGDGQAEVVYSDEIYFRIYEGKTGDVLFQTCNTTGTLIEYPLVAD
                     VDNDGQADIVVVSNAYAYDCGGTSQSGLRVFGPATGSWVRTRRVWNQHAYHVTNIEED
                     GTVPLHELPNWKQPGLNNFRQNKQPGNEFSAPDAVLELSIVCAPPALAALVRNIGEAS
                     MPAGLEVSFYRGTPPSGDFIGAASTTKPLYSAQSEVVRLALPSAFEGLLNGKDSAYAV
                     VDVPTSVHECRSDNNTSAPVTAHCSTPK"
     gene            complement(2633..>3752)
                     /locus_tag="PK710_21765"
     CDS             complement(2633..>3752)
                     /locus_tag="PK710_21765"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT12422.1"
                     /translation="EAMGARPPRLHTRHGDPASEILREAKDCNADLIVMGSQGIGGLN
                     VFSLGSTAARVVRQASCPVVTWRADDSLEKIDQAFRKVYSSLSVASLRAPDEDPAEAA
                     GLFRHHGTEYYLGYYTRSGFVHVLEQYGIFGLLRDRGYDPKVSFDLSDPFEHVLRIHD
                     AGIEDRDHLLIEVALRPSVIELPGQNPPQHFDVLLVLWLCLQDPRGSFSLRKPPMPDQ
                     SYPGLGLAREMMELLVLMAERLGKRALVNRPMNLHNARYYHHKFHFLDPRMEGRLAAI
                     LRDTEDTSLADASWAMQLGCVVDTHSGEPFRWEGKEQVFPMCDELRAYFDDIEYRRQV
                     WATALEKHYEIDWEQFRQRIQDPSVMASLTTSGCCNAR"
BASE COUNT          799 a         1010 c         1109 g          834 t
ORIGIN      
        1 gggatcatcg ctacaagagc cttctcgcaa caaatcctcc ccttcttcaa acgtgatcat
       61 ctcgaccgcg cgttgcccca gcaaccaacg atacggctca agagccgcct gttccgcttc
      121 ccctgccagt cgtacgtttg gcagttcata catcgacttg ttgactccgt cccatggtca
      181 gaaacgacga tccgttgctc ctgctatcat agtcgtacgc cccataaagg caattatccc
      241 ccatcacccc ttggacattt gtaagaaatc taacaatcca cacaatctat ataatctata
      301 ttatttgcac aagaattcct tgtccgcggg gcgtttacag ggtagcaata gtgtatgcgc
      361 ctttttatct tttttacctt gtcgtgggtc gtggcttcgt tacccgcctg ctcttgtggt
      421 ggcgaggaag acagtacagc ttcccctcag aagggttccg acggaggatt caaggatgcc
      481 gccgacagcg gcacggacgc cccctccgat acgaatctcg accaattttt ccccttcgat
      541 gccgacgaat gtgtcaacgg acaaccttgc ggggatggcg ggatttgcgc gggcaatacc
      601 tgttgtagca ccgagaaagc ttgtggaaat gcttgttgtg gagatagtca aatctgttcc
      661 ttccaggttt gcgtcacacc aggcaaggat tgcttggatt cttcagaatg tgaagataat
      721 gaatactgcg aatacggact cggcgagcca atggaagcag gcgttccgga tagcggttgc
      781 tccggaggcc atatcccctt gcagggaaag tgcgttccaa ggccacccca atgctccgaa
      841 gtcgatggag gcagcccgga cggtgggccg ctgacctgtc tggacgagtg caagtgggtc
      901 ccggaaacca cggattttca agcggaactc aagtacgcct ggggcggcat gacttctccg
      961 ccctacgatt ccgatatcat gatgtcgccg atcgtcattc aactcgatga cgatgattgc
     1021 gatgggaaag tcaccgccaa cgatattccc gagattgtgt tcaatacgtt ttcaggaggg
     1081 gcgtatacgg gcataggaac ggtgcatgcc atctcgatcg tgcaaggaca ggttgtggaa
     1141 aagtggtccg ttccgggagt attcaacgcg tcatcggagt tggcgggggg caatatcgat
     1201 ggcgaaccag gcaacgagat cgtgggttgc ggcgccgggg cggtgattgc gcttcgggcg
     1261 gatggtacgg agctatggcg aaacgcaagc gggctggctt gtcgggttcc agctatcgcg
     1321 aacgtggacg gcgatggcac cccagaggtt atcgtggagg gagggattct tgatggagcc
     1381 acaggcgaaa ccaaggttgc ccttccttct catccgtgga ttgccgcgga tttggacgat
     1441 gatgggcaac tcgacttggt cgggcctcaa ctcgccgttc atgcggacgg aacgcagttt
     1501 gtagatacag gaatggccgg tactttcgcg gccgtggggg atttcgacct cgatgggaat
     1561 cccgaggtcg tggcggtcaa tacgagcaat cacacgatgt gggtgtggcg ccatgatccg
     1621 tcgtctgcca atggagccgt catcgtgcgt gaccccatcg acatcaatgg cgcgttggat
     1681 ccagcgctct gtccgccggg atcgtcggga gccacgcatg gcggcgggcc tcccacgatt
     1741 gcggatttcg atggggacgg cattcccgat gtggcccttg cggggggggt tggatacgcc
     1801 attctgagcg gcgccaagct catggatccg gccgtagcgg gtccagacac atttttatgg
     1861 gtcaaacaaa cgcgcgattg ttcttcggca gcgacgggga gttccatttt cgacttcaac
     1921 ggggatgggc aggcggaagt cgtgtattcc gacgagattt acttccgtat ttacgaagga
     1981 aaaaccggtg atgtcttgtt ccaaacctgt aataccacgg gcacactcat cgagtatccg
     2041 ttggttgcgg acgtggacaa cgatggtcaa gcggatatcg tcgtggtttc caacgcgtat
     2101 gcttatgatt gcgggggcac gagccaatcc ggactgcggg ttttcggtcc cgccacgggt
     2161 tcctgggtgc ggacgcgtcg ggtgtggaac cagcatgcgt accatgtgac gaatatcgag
     2221 gaagacggga cggtgccgct gcacgaactc cccaattgga agcagccagg attgaataat
     2281 ttccgtcaaa ataagcaacc gggcaatgag ttttccgcgc cggatgccgt gctcgagctt
     2341 tccatcgtat gtgctccgcc ggccttggcg gcgctcgtcc gcaacatcgg tgaagcctcc
     2401 atgcccgcgg gcctcgaggt atccttctat cgcggcacgc caccgtccgg ggacttcatc
     2461 ggagccgcct cgaccacgaa acccctgtat tctgcgcaat ccgaagtcgt ccgtctcgcg
     2521 ttaccctcgg ctttcgaagg gctgctcaac ggcaaggata gcgcgtatgc ggtcgtggat
     2581 gttccgacct cggttcatga gtgccgatcg gacaacaata cctcagcgcc cgtcaccgcg
     2641 cattgcagca ccccgaagta gtcagggatg ccatgacgga cgggtcctgg attcgctgac
     2701 gaaactgctc ccaatcgatt tcgtagtgtt tttctagcgc ggtcgcccaa acctgccttc
     2761 ggtattcgat atcatcgaaa tacgctctta attcatcgca catcgggaaa acctgctctt
     2821 ttccttccca acgaaacggc tctcccgagt gggtatcgac cacacatccg agttgcatgg
     2881 cccaagaagc atcggcaagg gaagtgtctt ccgtatctcg gagaatagca gcgagtcgtc
     2941 cttccatccg tggatcgaga aagtgaaatt tatggtgata ataccgggca ttatgcaggt
     3001 tcatgggccg attgacgagg gctcgtttgc ctaagcgctc ggccatgagc accagcagtt
     3061 ccatcatttc ccgagccaat cccaatcctg gataggattg atccggcatg ggtggtttac
     3121 gaagggagaa ggagccccgg gggtcttgca ggcagagcca gaggacgagc aggacatcga
     3181 agtgttgggg gggattttgc cctggcagct cgatcacgct aggccgaagg gcgacttcga
     3241 tgagcaaatg gtccctatct tcgatgccag cgtcatggat gcgcaagaca tgctcgaaag
     3301 gatcggatag gtcgaaagaa accttgggat cgtagccccg atctcgaagc aagccgaaga
     3361 taccatattg ttcgagcacg tggacaaagc cggaacgggt ataataaccg agatagtatt
     3421 ccgtcccgtg gtgccgaaat agtcccgcgg cttctgcggg gtcttcatcg ggggctcgca
     3481 aggaagctac ggaaagggag ctatagactt ttcgaaaggc ctggtcgatt ttttcgaggg
     3541 aatcatcggc gcgccacgta acgacggggc agcttgcttg tcgcaccacg cgagcggcgg
     3601 tcgaaccgag ggaaaatacg ttcaacccgc cgattccctg gcttcccatg acgatgaggt
     3661 cggcattgca atctttggct tctctaagga tttcgctcgc ggggtcgcca tgtctcgtat
     3721 gcagtcgagg gggccgggct cccatcgctt ca
//
LOCUS       DAOSOR010000570         3751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_24572_length_3751_cov_4.395563, whole genome shotgun sequence.
ACCESSION   DAOSOR010000570 DAOSOR010000000
VERSION     DAOSOR010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24572_length_3751_cov_4.395563"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PK710_21770"
     CDS             <1..191
                     /locus_tag="PK710_21770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT12423.1"
                     /translation="EPYVTTKMHGTGLGLSIVQRIVIEHGGEIAYEPQPTGACFAILL
                     PCAGPVATETTSSIPLGE"
     gene            250..2862
                     /locus_tag="PK710_21775"
     CDS             250..2862
                     /locus_tag="PK710_21775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12424.1"
                     /translation="MQSRSFLYVAPIALVVCSLSCGGSAPSRLSPSSAPAATAPTPDA
                     HDDGLASLITSVPEGTLGPYVGYGPKGALALFSPPHESQRRWWVQALDEQGKPKAEPF
                     GLGPSPDDVPFATVRPIGEDVFGAIWVRRIHHTDVLEGALVKNNSMLSDNITTFTQAR
                     GKVIWADAVATSKATILLWVEQQGEETQLQRLLLNPKGEPIGQSEVLQRNVRAWDTVA
                     IDDGMAVALVIGLEEKKNLGSVALLTLDAAGHPKGAVTPISHDGSARFDIGLVRAGQR
                     LLLAWTEVRQADSVIRAAVANFEGRLLVEPRTPRQSLGGQALVALVAPAASKPNQALL
                     VFEEFPSSNQPQRELRMTTLSDEAIASSEGVRLAFSPVGQPNGEYATSSDGFVFLTRA
                     QACAKNGACDDIPWYVRLSSSLTIDAAAPVVVSALHYEPPVLMWSPGCTSMTCLALAA
                     GGQDPTPIVMAKLPKGALQPNTFLVREPAPKPPFVERSQTLLTSIEPLSAFDAATAGS
                     TTYVGWVTHFVEGQGIPQQAPRQAPGNPNKPTAAHLLVQRIDPSGMPLEGPTTVSVRA
                     MSRGGIALAPHPTAQEVCVAWTARDAGTPQVFLTRIGADGKTKLQRMITHGKGDVSDV
                     AIAAHGDGWIVAWVDWRDGQGNVYAARVGASLIKLGPEKLISRAAGDASDLSLAVMGD
                     LVFMAYGSSRADIPNGISNPTIRKLNAKTLEPIGEEQIIDSSALPVHLSKLSVSGHDL
                     VLAWMAQGSPDCEAASCAFARASRIDPDTLRVLTTADFRKSGTTPASLSLRCDEQRCQ
                     GVLLAPHAGKLDLFGLTWVPTSTAMQRNELGSITSPSVGALPPAMVGDALFLADQGLE
                     GQDRLRRIQVRWTP"
     gene            complement(2937..3027)
                     /gene="ffs"
                     /locus_tag="PK710_21780"
     ncRNA           complement(2937..3027)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK710_21780"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3157..3741)
                     /locus_tag="PK710_21785"
     CDS             complement(3157..3741)
                     /locus_tag="PK710_21785"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HOT12425.1"
                     /translation="MKRKQDLERARRGIEEFLRGLGFDIEDHPELARTAERVVGAFSQ
                     DLLSGESMDDATMLKKGSSPARPGEKGIVVLRNLSTVTVCPHHLMPAIGHATVMYVPD
                     KRIAGLGAVARLVDGHARRLVLQEEIGERVAQALVEHLGARGALCRLSLVHTCLVARG
                     ERKHDAHVDTVAFAGSLAVPGPDRELAMMELHTR"
BASE COUNT          785 a         1179 c         1030 g          757 t
ORIGIN      
        1 tcgaacccta tgtcaccacc aagatgcacg gaaccggatt gggcttgtcg atcgtacagc
       61 gcatcgtcat cgagcacggc ggcgaaattg cctatgaacc ccaacccacc ggagcatgct
      121 ttgccatctt gctgccctgc gcgggccccg tcgccacgga aacgacttcc tccatccctt
      181 tgggagaatg aattgtgccg ctcgacggtt agcccccata atcttgtctc caccctttga
      241 acgagtccca tgcaatcacg ctcttttctc tacgtcgccc ccatcgcttt ggtggtttgt
      301 agcctttcat gcggcggcag tgcgccctct cggctttccc cctcttcggc ccctgccgcc
      361 acggctccca cacccgatgc gcacgacgac ggccttgcct cgctcatcac cagcgtgccg
      421 gaaggcaccc tgggacctta tgtagggtat ggccccaaag gtgccctcgc tttattctct
      481 cctccccacg aatcgcaacg acgatggtgg gttcaagccc tcgacgagca aggaaaaccg
      541 aaagcggaac cctttgggct cgggccaagt ccggatgatg tgcccttcgc gaccgtccga
      601 cccatcggtg aggacgtttt tggagcgata tgggtgcgaa ggattcacca taccgacgtg
      661 ctcgaaggcg cattggtcaa aaataacagc atgttgtcgg acaacatcac cacattcacc
      721 caggcgcgcg gaaaggtgat ttgggctgat gcggtcgcca cttccaaagc cacgatcctg
      781 ttgtgggtgg aacaacaagg cgaggaaacg caattgcaac gcctgctgct gaatcccaaa
      841 ggcgagccga tcggacaaag cgaagtcctg caacgcaatg tcagggcatg ggataccgtc
      901 gccatcgacg acggtatggc cgttgccctg gtgattggtt tggaagaaaa gaaaaacctt
      961 ggttccgtgg cgttgctgac gttggatgcc gcgggacacc ccaaaggagc tgtaaccccc
     1021 atctcgcatg atggctccgc tcgattcgat attggcctgg tgcgtgcggg gcagcggcta
     1081 ctgctcgcct ggacggaagt ccgtcaagcc gatagcgtga ttcgagcggc ggtagccaat
     1141 ttcgaaggcc ggttgttggt ggaaccgcgt acgccacgac aatctctcgg gggtcaggcc
     1201 ctggtggctt tggtagcgcc tgcggcatcg aaaccaaatc aagctctctt ggtattcgaa
     1261 gaattcccat catcgaacca gcctcagcgc gaattgcgga tgacgacact cagtgatgag
     1321 gcgatagctt cctccgaggg ggttcggctc gccttttccc ctgtgggtca acccaacggg
     1381 gaatacgcca catcctccga tggattcgtg tttctcacac gcgcgcaggc ctgtgcgaaa
     1441 aacggggctt gtgatgatat cccttggtac gtccggttga gttccagcct aaccatcgac
     1501 gctgcggcac ccgtggtggt tagcgccttg cattacgagc cgcccgtatt gatgtggtcg
     1561 cccggttgta cttcgatgac ctgccttgcc ttggctgcgg gcggacagga tccgacaccc
     1621 atcgtcatgg caaagcttcc gaagggtgcc ttgcaaccca acacgttttt ggttcgtgaa
     1681 ccggcgccca aacctccctt cgtggaacga agccaaaccc tattgacatc gatcgagccc
     1741 ctttccgctt tcgatgccgc cacggccggg tctacaacct atgtcggatg ggtgacgcat
     1801 ttcgtcgaag ggcaagggat accgcaacag gcgcctcgcc aagcgccagg aaacccaaac
     1861 aaacctaccg ccgcgcatct gctggttcaa cgcatcgacc cgtccggcat gcccctcgag
     1921 ggccctacca ccgtttccgt tcgcgccatg tcaaggggcg gcatcgccct tgcgccacac
     1981 cccacggccc aagaagtttg tgtggcgtgg acagctcgcg atgccggcac gccgcaggtg
     2041 tttttgacgc gaatcggagc cgacggcaaa acaaaattgc aacgcatgat cacgcatggc
     2101 aaaggagacg tctccgatgt cgcgatcgcg gcgcatggtg atgggtggat tgtcgcttgg
     2161 gtcgactggc gcgatggcca gggcaatgtg tatgccgcgc gggtaggcgc atccttgatc
     2221 aaacttggtc cggaaaaact catctcgcgg gctgctggcg atgcttccga cctcagcttg
     2281 gccgtgatgg gagatctcgt cttcatggcc tatggcagct cccgggcgga tataccgaat
     2341 gggatatcca atccaaccat tcggaagctg aatgccaaaa cgctggaacc tattggagag
     2401 gaacaaatta ttgacagctc tgccctaccg gtacaccttt cgaaattatc cgtatcggga
     2461 catgatttgg tgttggcatg gatggcgcaa ggatccccgg attgtgaggc ggcaagttgc
     2521 gcgttcgcgc gagcgagccg aatcgatcct gatacccttc gggtccttac gacagcggat
     2581 tttcggaagt caggaacaac gcctgccagc ttgagcctgc gctgcgatga acaaaggtgc
     2641 caaggcgtct tgctggcgcc tcatgctgga aaactcgatc tattcggcct cacctgggta
     2701 ccgaccagca ccgccatgca gcgcaatgaa ttgggatcca tcaccagccc atcggtagga
     2761 gcgctgcctc cggccatggt gggcgacgcg cttttccttg ccgatcaagg actcgaagga
     2821 caagatcgct tgcgacgaat ccaggtgcga tggacaccgt agggcatgaa cagctcccct
     2881 cccccacgag cccaagcatc catggttgga gcgtccgacg attcgatgca ccacaaacgg
     2941 gaaaggcccc gtcacacccg ctctctgcta ccgttgcttc cttccggacc tggcgggttc
     3001 acagcgcgct gtcgacgagg ccatacattt cgaactgagt gattgtgtgt agcgcatcat
     3061 cctggctttg tcgagggaga tgacgaacgc tgtcgaaccc agccaaaaac gtgggaggca
     3121 gggaaagcaa tcccgttatt tcatcggtat ttgccgtcaa cgcgtgtgaa gctccatcat
     3181 cgcaagctct cgatccggcc ctggtacggc cagagaaccc gcaaatgcca cggtatccac
     3241 atgggcgtcg tgtttgcgct cccctcgcgc caccaaacac gtatgaacca gcgacaaccg
     3301 gcaaagcgcc ccccgcgcgc caagatgctc gaccaacgct tgcgccaccc gctcaccaat
     3361 ctcttcttgc aacacgagcc gcctcgcgtg accgtccacc aaacgcgcca ccgccccaag
     3421 ccccgcaata cgcttatccg gtacgtacat caccgtcgca tgcccaatgg ccggcatcaa
     3481 gtgatgagga caaaccgtca ccgtcgatag attgcgcaac accacgatcc ctttttcccc
     3541 aggacgcgcc gggctggagc cctttttcaa catcgtggcg tcatccatac tctcaccgga
     3601 caacaggtct tgggaaaaag ctcccaccac ccgctcggcc gtacgcgcca attccggatg
     3661 atcctcgata tcaaaaccaa gcccccgaag gaattcctcg attccacgcc gggctcgctc
     3721 gagatcctgt ttgcgtttca tgactcgact c
//
LOCUS       DAOSOR010000571         3740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_24661_length_3740_cov_5.883582, whole genome shotgun sequence.
ACCESSION   DAOSOR010000571 DAOSOR010000000
VERSION     DAOSOR010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24661_length_3740_cov_5.883582"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="PK710_21790"
     CDS             complement(<1..889)
                     /locus_tag="PK710_21790"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HOT12426.1"
                     /translation="MRSRLRSCLLVAFAGAACSSYEPDFELVAQRFDPIINGTLDTST
                     SNDSIVMVFHQGPYYQSICTGTLIAPNIVLTARHCVSNTNEYVDCVNDVTGEFSASEL
                     YILKGYDPLSGNPQAIAQGAQVYHDGSKGLCGHDLALIRLSSPISSLTPVRVRVSSAP
                     TIGETFKAVGYGLTNPNNPNSSGRRYYRDGVTVSQIVYGNDFRGGQSICSGDSGGPAI
                     SQQGAVFGVTSRGADCYGNDNYWTRTDSFKSLIDQAATAAGTTYTGEDGTIYPGGGGS
                     GGSGGSGGSGGSGGSGGSGG"
     gene            complement(1442..1807)
                     /locus_tag="PK710_21795"
     CDS             complement(1442..1807)
                     /locus_tag="PK710_21795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12427.1"
                     /translation="MIHPSPHPSRFPSYPEDTNDTGVRQRTIFFQSVADIDARVRTQR
                     ASLDGALLTVANGSRFVLRDALRVLGSSKGHDVFGMTGRIMPLSELLTMGATLSSSTL
                     QIGNVHYEVQLGYLVQPLG"
     gene            complement(1983..>3740)
                     /locus_tag="PK710_21800"
     CDS             complement(1983..>3740)
                     /locus_tag="PK710_21800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOT12428.1"
                     /translation="CQTLFMEPRAIIALGNKLKVVDAQIEREQVAITTRLSEMIREQI
                     EAVAAAESALAHAELKAAAAVLAQDLDLCFPHFEPTPVMDLIQARHPLLQLDGVKVIP
                     SDMKVESGHALIVSGPNAGGKTVVLKTLGLAACMIRAGLPIACDPRSRVGLFDRVATD
                     MGDDQSISKNLSTFSAQVRNLAGILETADVSTLVLLDEIATGTDPRQGEALATGVLDG
                     LCARGAAVACTTHYEGLKALALGDDRFRNASVGFDMSSMSPTFSLATGLPGASSALAI
                     ARRFGMPSYVLERAQQYLAVAEQGFDSLVAKLNDERRALELARSAAEREKQWAEARRR
                     ELDAELKQQRSREHKLLSEHTQGLLSAIQRAREELRMAQQRLRSKKLNEEALKEIKRT
                     VDAVAQKVAMGGELEPVQAKTTASTTPLSPEMIGPGQRVYVPRLRAEAQIVDVLKQGQ
                     VRVALGAVKVLAHIDELRGVGASEDTPAPARNVRPNVKPARFFDAAADPDLPLQTSDN
                     TCDLRGLRAEEAVAMAEQFLDRCLNEGRRVAFLIHGHGTGALRNAIRSALQTNGYVQR
                     FRPGQSGEGGDGVTVAWLV"
BASE COUNT          790 a         1103 c         1047 g          800 t
ORIGIN      
        1 aaccgcctga gccacccgaa ccgcctgagc cacccgaacc gcctgagcca cccgagccac
       61 ccgatcctcc ccctcctgga taaatcgttc catcttcgcc cgtgtaagtc gttcctgccg
      121 cggtcgccgc ctggtcgatc agacttttga atgagtcggt tcgtgtccaa taattgtcgt
      181 tgccgtaaca atcggccccc cgcgacgtca caccaaatac cgctccctgc tgcgaaatgg
      241 caggtcctcc cgaatctccc gagcagatcg actgcccccc acgaaaatca ttgccataaa
      301 cgatctgact caccgtcact ccgtctcgat aatatcgtcg ccccgatgaa ttcggattgt
      361 tcggattcgt cagcccatag cctacggcct tgaacgtttc cccaatggtg ggcgcagacg
      421 agacacgcac acgaaccggt gtgagcgacg atatcgggct ggaaagccga atgagggcaa
      481 gatcgtgacc gcaaaggccc ttgctcccat catgatacac ttgcgctcct tgggcgatgg
      541 cttggggatt tcccgaaagc gggtcgtaac ctttgaggat atacaattcg ctggcgctga
      601 attcccccgt gacgtcgttg acgcaatcga catactcgtt ggtgttggac acgcaatgcc
      661 gcgccgtgag cacgatattg ggagcgatga gcgtgcccgt gcagatcgat tggtagtatg
      721 ggccttggtg aaacaccatc acgatggagt cattcgacgt gctggtgtcg agcgttccgt
      781 tgatgatggg gtcgaaccgc tgggctacca gctcgaaatc cggctcgtag gaactgcaag
      841 ccgcacccgc gaatgccacc agcaagcacg atcgaagcct cgagcgcatc tttcaatcat
      901 aacggtttag cgatggttgc accacggcta cagaaaagat gctgcgctcg gataagacac
      961 cgtgcgacac tgtaagcctg ataccgaccg aatcgaatca ttggaaagcg gcattgctct
     1021 agacttgcat ttccggcaaa atcacttcgt atgggccttc gggagccatt ttgttgcgcc
     1081 tttttcctca gcgtcgcgag caattccctg ggttgcgcga atgtgctggg attcgacgat
     1141 atctctttcg agcaaggaga tggcgtagcc cgtgggcccg ttacctcagc ggaatgtggg
     1201 aagatatcag cgtcttcgat ggcatgcgcg acttgcgtca gtgatttctg ctgcgatatc
     1261 gccaaagcat gcggcgagca aacaccgtgc gcacggctgg cggcctgtcg aatgagatgc
     1321 acggtcgacg atatcgcatg cttgcacgat tgtggggtcc aacatttcga tgggatgacg
     1381 tcgtggaagg aattggatgg gtgcgagtca caaagctgtg cgcaaacgtg cgttcgatga
     1441 atcaaccgag gggttgcacg aggtagccca gttgcacctc gtagtggacg ttaccgattt
     1501 gtagcgtgct cgacgatagc gtggccccca tcgtcagcaa ttccgaaagc ggcatgatgc
     1561 gccccgtcat gccgaaaaca tcatgccctt tcgaagatcc gagaacgcgc aacgcatcgc
     1621 gaagaacgaa ccgagaaccg ttggctaccg tgagcaatgc gccgtcgaga ctcgcgcgct
     1681 gcgttcgtac ccgcgcgtcg atatccgcca cggattggaa aaagatggtg cgctgtcgca
     1741 cacccgtatc gttcgtatcc tccggataag acgggaaccg actgggatga ggagaaggat
     1801 gaatcatgcg tccttggtgg tggttacgaa agtaaccgca tagccccgaa cagaagcagc
     1861 cttcgtgcca atgaaccctg gatctggagg agtcggtaaa ataatatgta aaaacaagtg
     1921 gttatcgttt gcgcctagag gttggggaag gcccaatcca cgccgattgc ggttgaatca
     1981 tgtcagacaa gccacgcgac cgtgacacca tcgccaccct cgccgctttg accggggcgg
     2041 aaacgttgca cgtaaccgtt cgtctgaaga gcggagcgaa tcgcgtttcg caacgctccc
     2101 gtgccatgac catgaatgag aaaagccacc cgtcgcccct cgttgagaca tcggtcgaga
     2161 aattgctctg ccatggccac ggcctcttcg gcacgcaatc ctcgtaaatc acaggtgttg
     2221 tctgaagttt gaagcggcaa atccgggtcc gctgcagcgt cgaaaaagcg tgcgggcttg
     2281 acgttgggac gaacgttgcg ggcgggagcg ggcgtatctt cggatgctcc tacgccccta
     2341 agctcatcga tatgggccag gactttgacc gcaccgaggg cgacacgcac ttgcccttgt
     2401 ttgagcacat caacgatttg cgcctctgcc cgcagtctcg gaacatacac gcgttggccc
     2461 ggcccaatca tctccggcga caacggtgtc gtggatgctg tggtcttggc ctgcacaggt
     2521 tcgagttctc cgcccatcgc aactttttgc gccacggcat cgacggttcg cttgatttct
     2581 ttcagcgctt cttcgttgag ttttttgctt cgtaagcgct gctgcgccat gcgaagctcc
     2641 tctcgcgctc gctgtatggc agacaacaat ccttgtgtgt gttcggacaa taatttgtgc
     2701 tctcgacttc gctgctgttt cagttccgca tccaactccc gtcgacgagc ttccgcccac
     2761 tgtttctctc tttccgcggc cgaccgggcg agttccaaag cccgacgctc gtcgttgagc
     2821 tttgccacca ggctgtcgaa tccctgctct gccacggcca ggtattgttg cgcgcgttcg
     2881 agaacgtacg atggcatgcc gaagcgacgg gcgatcgcaa gcgcgctgga ggctccgggc
     2941 agccccgtgg ccagcgaaaa cgtcggactc atcgaagaca tatcaaaccc gaccgatgca
     3001 ttacgaaatc gatcgtctcc caaggccaac gctttgagtc cttcgtaatg cgtcgtacaa
     3061 gctaccgccg ccccacgcgc acaaagaccg tcgagcactc ctgtagcaag cgcttcgcct
     3121 tgccgcggat ccgttcctgt cgcgatctcg tcaagaagca ccaacgtgct aacgtccgcg
     3181 gtttcgagaa tgccggccaa gtttcgaacc tgcgcgctga acgtcgatag gttcttcgaa
     3241 atgctttggt cgtcgcccat gtcggtggcc accctgtcga acaatccgac gcgagacctg
     3301 gggtcgcaag cgatgggaag gcccgcgcgg atcatacatg cggcaagacc aagggttttc
     3361 aggacgactg tttttccccc cgcgttggga ccggaaacaa tgagtgcgtg cccggactcc
     3421 accttcatgt ccgaggggat cactttcact ccgtcgagtt gaagcaaggg gtgacgcgcc
     3481 tgaatgagat ccatcaccgg cgttggttcg aagtgaggaa aacacaagtc cagatcctgt
     3541 gccaggaccg ctgcggccgc tttgagttcg gcgtgggcaa gagcggattc cgcagccgct
     3601 acagcctcga tctgctctcg gatcatctcc gacaaacgtg tcgtaattgc aacttgctcg
     3661 cgctcgatct gcgcatcgac caccttgagt ttgttgccca gcgcaatgat ggcccgcggc
     3721 tccatgaaca aggtttgaca
//
LOCUS       DAOSOR010000572         3729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_24752_length_3729_cov_3.942297, whole genome shotgun sequence.
ACCESSION   DAOSOR010000572 DAOSOR010000000
VERSION     DAOSOR010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24752_length_3729_cov_3.942297"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PK710_21805"
     CDS             complement(<1..772)
                     /locus_tag="PK710_21805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12429.1"
                     /translation="MTRQLELKTVVRHWIFLTLAILVFTLTACTKKGDSIVLAGDGTR
                     LSSEEIDRDPLALLPSGVAGLVHIDARTALQSELGRPTTKLIQSMMPLGPESNFDPGR
                     DVRRVIVGLYSLQGADVAVVVQGSFDPDAIRMAASSGRPTALGVPLTRLEYANNDLFV
                     AGDVGFVVVTKQTLILGNETGIRRTLDRIRDKRVRRDVPDWMASLIDDPKASVVAVAD
                     LSTDPIVHSAAQQTPFLHGLTVVRILGNFEPPGLNLAGT"
     gene            complement(801..1871)
                     /locus_tag="PK710_21810"
     CDS             complement(801..1871)
                     /locus_tag="PK710_21810"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HOT12430.1"
                     /translation="MSNRKKVDHLRAQMADTDKEVLRLLEIRAKMARDVGTLIKDDPS
                     ASHGLDRTYFDDLIAHGTGELPDESVKQIFKAIYAACASLERPARVAVVGVEGSLGYV
                     AARQVFGGGAQPSFCDSPSAALEEVTRGRAEFALVPFESSHEGTVVSSILALMQTDLV
                     VSAICELAAGVALLNHTGNPEDIEKIYATAHDRVHCESFLHKRIPRATIIDVRSPAIG
                     CRFAVEDHGGATIAHEQMGDIHGLTIAIANIGEQHDLRMRYAVVGTRLSSRTGKDTTS
                     IVFTLNDQPGALAAVLNDFATRGINVKSIMSRPVPGEKWDYVFYLDVHGHASDRGLVI
                     ALDEIRKYTKLMKIIGSFSTHC"
     gene            complement(1924..2166)
                     /locus_tag="PK710_21815"
     CDS             complement(1924..2166)
                     /locus_tag="PK710_21815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12431.1"
                     /translation="MLRSFLLAIAVALAPMQCQGSEDPSLQRTENPGEALYDLAQEFR
                     AKGQSKAYEDTLRFLIARYPSSRRAVTARSELEALK"
     gene            2385..>3729
                     /locus_tag="PK710_21820"
     CDS             2385..>3729
                     /locus_tag="PK710_21820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12432.1"
                     /translation="MSFMNKLAAWGLASCGMVALISSCSANSSQDKYSGTGGGIAEAG
                     DDVTDASAGSGGSSTDSSTDGLFVTDGSLTDVVNETPINICGSECGNTELCDPQHLGY
                     DDNCNGQVDEGCPCTPGLSHWCFKGDPASRNKGACKDGVQKCGETGTWGPCIGGFHAD
                     AIDNCLNQNIPQGCHDLSVAPFTTVHLKDGTGTFGQGATGESFEVTCPSSIPPANCPH
                     VQEPNSPNATYTPLQLGQYEVLYSKDDGAGGKATCTFSLYVGATGLRVELNWDHQGEG
                     RAGTDSEGKPNGTFGPDLDLHVHKPGVQTKWFMNPLQPMAPQDDCYYANCTASNFAIA
                     MPMMAAPKWFFDDAYPHNWTYRLPYNPNDPIYTCYQAPRGAGDEWRVFKKGCHNPRLD
                     LDNVVCDNTKTDPQDPDFCAPENVNIDEPPFGLWTRIGVHYYGHCYAGDLHPTVTI"
BASE COUNT          885 a         1007 c         1046 g          791 t
ORIGIN      
        1 aagtaccagc gagattcagc ccgggaggct cgaaattacc gagaatgcga acgaccgtca
       61 gtccatggag aaagggcgtt tgttgtgcgg cgctgtggac aatgggatcc gtgctcaaat
      121 ccgctacggc caccaccgaa gctttggggt cgtcgatgag cgatgccatc caatcaggaa
      181 catcgcgacg aacgcgtttg tcccgtatcc ggtctagcgt tcgacggata ccagtttcgt
      241 tgcccaggat gagggtttgt ttggtgacga ccacgaatcc gacatcgcca gccacaaata
      301 aatcgttgtt ggcgtattcg agccgtgtga ggggaacgcc aagagccgtc ggacgtccgc
      361 tcgaagcagc catgcgtatc gcgtcgggat cgaaactacc ttgcacgacc acggcaacat
      421 ctgcgccttg cagcgaatac aggcccacga tgacacgacg aacatcgcgt cccggatcga
      481 aattcgactc cggacccaat ggcatcatgg attgaatcag tttggtggtg gggcgaccaa
      541 gctcggattg cagcgcggtt cgcgcatcga tgtgaaccaa gcccgcaaca cccgatggta
      601 gcagcgccag cggatcgcga tcgatctcct ccgacgacaa tcgcgttcca tcgccggcaa
      661 ggacaatgct atcccccttt ttcgtgcatg ccgtcagcgt gaaaaccaag atggcgagag
      721 taaggaaaat ccagtgcctt acaaccgttt tcaattccaa ttgtcgagtc atgatagggc
      781 ccccatcgaa gggttatgct tcagcaatgg gtggaaaaag aaccaataat cttcattaat
      841 ttcgtatatt tacggatttc gtccagagcg atgaccaacc ctcgatccga ggcatgacca
      901 tgcacgtcga gataaaaaac gtagtcccat ttctctccgg gaacaggtcg cgacatgatg
      961 cttttcacat tgatgccgcg tgttgcgaag tcattgagga cagccgccaa ggctcctggt
     1021 tgatcattga gcgtaaagac aatggacgtg gtgtcttttc cggtgcgtga ggatagccgt
     1081 gttcctacca cggcgtaccg cattcgaagg tcatgctgct ctccaatatt ggcgatggcg
     1141 atcgtcaatc catgaatatc gcccatctgt tcgtgagcaa tcgtcgctcc cccatgatct
     1201 tccaccgcga accggcagcc aatggccgga gaacggacat cgatgatcgt ggcgcgcgga
     1261 attcgcttgt gaaggaacga ttcgcaatga acgcgatcat gggccgtcgc gtatattttt
     1321 tcgatgtctt cgggattgcc ggtgtgattg agcaaggcaa ctccggccgc gagttcgcag
     1381 atggccgaca ccaccagatc cgtttgcatg agggccaaaa tgctgctcac caccgttcct
     1441 tcatgggagg actcgaaagg aaccaaagca aattcggcgc gtccacgagt cacttcttcg
     1501 agcgccgccg atggtgaatc gcaaaacgat ggttgagcgc cacctccgaa aacctgtcga
     1561 gcggccacat acccgagcga tccttcgaca cctacgacag ccacacgagc aggtctttcg
     1621 agagaagcac aagccgcata aatggctttg aaaatctgct tcacgctttc atccggaagc
     1681 tcgccggtgc cgtgcgcaat caagtcatcg aaatacgttc ggtcgaggcc atggctagcc
     1741 gacggatcgt ctttgatgag cgttcctacg tctcgcgcca ttttcgcccg aatctcgaga
     1801 agacggagca cttccttgtc cgtatccgcc atttgcgcgc gaaggtggtc cacctttttt
     1861 cggttcgaca tgggggcaag cgtaatgcga ggacacgaga agggcaaagg ggaagcagac
     1921 ggttcactta agcgcttcga gttccgaacg tgcggttacg gcacgacggc tcgacggata
     1981 ccgggcgatg agaaagcgca gcgtatcttc atatgctttc gactgccctt tcgcccgaaa
     2041 ctcttgtgca agatcgtata aggcctcgcc ggggttttcg gtccgttgca acgaagggtc
     2101 ttcgctgccc tggcactgca tcggagccaa ggccacggcg atcgcgagaa gaaaagagcg
     2161 caacacgggg cgacaatagc acgaaaggat gccagcagga ctgtgggata tgagcggcgg
     2221 atgtcacgct aggatcgagt cgatcgcctg ccgttgtcct ttgcctatcg cttttcgccc
     2281 ctccatttca atgcaggtcg gcctgccctt ggagactgcc aagcacgatt gtgcgtgaat
     2341 ctgccggaca atcgaggcat aaccagatac gatcggaggt tcgcatgtcc ttcatgaaca
     2401 agcttgcggc atggggattg gcatcgtgcg gcatggtggc gttgatatcc agttgcagtg
     2461 ccaacagtag ccaagacaag tacagcggaa ccggtggcgg cattgcggag gctggagatg
     2521 acgtcacgga tgcttcagcg ggttctggag gttcgagtac cgacagttcg acagacggat
     2581 tatttgtcac ggatggttcg ctgacggatg tcgtcaacga aacacccatc aatatctgtg
     2641 ggtcggagtg tggaaacacg gagctttgcg atccccagca tctcggatac gacgacaatt
     2701 gtaatgggca agtggatgaa ggatgcccat gcacaccagg tctttctcat tggtgtttca
     2761 aaggcgatcc ggcgtcgcgc aacaaaggag cttgcaaaga cggtgttcag aagtgtggag
     2821 agacgggcac ctgggggcct tgtatcggtg gatttcatgc cgacgcgatc gacaactgcc
     2881 tcaatcagaa cattcctcaa ggatgtcatg acttgagcgt ggctcctttc accacggttc
     2941 atttgaagga tggaacggga acattcggtc agggagcgac gggagaaagc ttcgaagtca
     3001 cgtgcccatc gagcattcca ccagccaatt gtcctcatgt gcaagagccc aacagtccaa
     3061 acgcgaccta tacgcctctt cagcttggac aatatgaggt gctgtattcg aaagacgacg
     3121 gcgccggagg aaaggcgaca tgcacgtttt cgctttatgt gggtgcgacc ggattgcggg
     3181 tggaactgaa ctgggaccat caaggagaag gtagggcggg aacggattcc gaaggcaaac
     3241 cgaatggaac ctttggccct gacctcgact tacacgtcca caaaccgggt gtgcaaacca
     3301 agtggttcat gaatccactg caacccatgg ctccccagga tgattgctat tacgcgaatt
     3361 gcaccgcgag caactttgcc atcgccatgc cgatgatggc ggcgccgaag tggttcttcg
     3421 atgatgccta tccgcacaac tggacctacc ggcttcccta caatcccaat gaccccatct
     3481 acacgtgtta tcaagctccc cgtggagccg gggatgaatg gcgggtgttc aaaaaaggct
     3541 gtcacaatcc acgactcgac ctcgataacg tagtgtgtga caacaccaaa accgatccgc
     3601 aagatccgga tttctgcgca cccgaaaacg tcaatatcga tgaacctccc tttggcttat
     3661 ggacgcgaat cggagtgcat tattacggcc attgctacgc tggggacctg catcctaccg
     3721 ttacgattt
//
LOCUS       DAOSOR010000573         3721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_24817_length_3721_cov_3.803873, whole genome shotgun sequence.
ACCESSION   DAOSOR010000573 DAOSOR010000000
VERSION     DAOSOR010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24817_length_3721_cov_3.803873"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="PK710_21825"
     CDS             <1..882
                     /locus_tag="PK710_21825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12433.1"
                     /translation="VQRGARLRLIDGKPVFYDRFVPLTLGPSAVNSVLASAHVRRIRS
                     GPTHPLLLPLDHSAELIGLEGARGARPALDRLTGKGMLVADLDSNADVFHPQFFHADA
                     GWFDWIDVNANGVFEPGIDAIDLDGDGIVGASETASWLKARTLSLHWGDEVPARDDVF
                     DPAIDWVYFDENGNQQRDYGVDQGFDDSTPAFGEPIFVPDDVNRNGKIDVGERFVRLG
                     TSKFKKSMSTSSTTPRSIMCLREDRTSARCNRTTPVVCMGIAMRCMLQACFRSSRRMS
                     PWWEDVGWGWRLKLICF"
     gene            786..2474
                     /locus_tag="PK710_21830"
     CDS             786..2474
                     /locus_tag="PK710_21830"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOT12434.1"
                     /translation="MHASGVLSIIAADVPLVGRRWVGMAPEADLLLGFEIAQDSAKSV
                     TWALGEKPDVMLHELSVWTGEPLDGSDPYSTMVDASVHNDHVTHTCPTGNLGGSRKHA
                     WLTISPGQTIVADLDVPSINATYVQTTFNVRGESNASIVLEEPNGTSHDLVTGPSYTN
                     LSTGALMYVGHTASSRGTYYWDALLYEHQAGKSIPTGAWKARIKNNGAAVMTVDAYCF
                     DEVSGWGLGVAWHPSIATDASTAGIPSVSDHCIAVGAHTGHPMSSSTPWFNTPGEVGA
                     VRDYSGRGPRIDGTQKPDVLAPDNPWAAAPNAAAFGGNIIPHGAVWPFGGTSGATPHV
                     TGVTALMAQAGVRGLDARQALRDSAIADSTTGEVPNFDYGWGRMDAAGALGALAAGKP
                     PEVQLFASSLSVAIDEPVTLTVSAVDPDGPSDQLEAKWDDGYDGSWDVPYAPIAPRTL
                     SFDTPGHYPTKVRVRDVQGRIDEAVVWIEVVESTHEPDAGADAQPAEGGETETGAGGS
                     SGNTGGSPSHPSSSSDDDDGCGCRVIASRDSAKHEYGWLVALAVFAARRRSSRG"
     gene            complement(2603..>3721)
                     /locus_tag="PK710_21835"
     CDS             complement(2603..>3721)
                     /locus_tag="PK710_21835"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HOT12435.1"
                     /translation="TVLFEDFLYALFAEVHRSRGGNRLGRVSAYLMQEAMTALSRRQA
                     FEVDSVIVGAMRIVHVSGIGPQDAMVVVAVEFEANVTERAQAGIAPQGRYLMERWTLV
                     RKHGPQSRVPAQAIVLACPSCGAPLDVVVAGHCSHCRQQVNSGAFDWTVRSIDLIRAE
                     YRGPMLTTNVAEQGTQLPTVFDPSLQQASAALTSRDPQFQWEGFRARIQLIFNEFQKA
                     WSARDLASMRPYLSDSLFQTQVYWVEAYRQQGLRNITERARIAGLEIVKIDADRFFDS
                     ITVRLWATGLDYTVTEDGQRVVSGSKSRERPYSEYWTFIRGTGRSAPTRTDKACPNCG
                     APLQINMAGVCEYCKAKVTTGQFDWVLSKIEQDEAYRG"
BASE COUNT          765 a         1085 c         1095 g          776 t
ORIGIN      
        1 gtgcaacgcg gggctcgatt gcgtcttatc gatggaaaac ccgtgtttta cgatcgattc
       61 gttccgctga cgcttggccc atcggcggta aattccgtgc tcgcctctgc ccacgtgcgt
      121 cgcattcgaa gtggccccac gcatccgctc ttgcttcccc tcgatcattc cgcggaactg
      181 atcggtttgg aaggcgcacg cggtgcaaga cctgccctcg accggcttac gggaaaaggc
      241 atgctcgtcg ccgacctcga tagcaatgcg gatgtatttc atccccaatt ttttcacgcg
      301 gatgctggtt ggttcgactg gatcgatgtc aacgccaacg gcgtattcga gccgggcatc
      361 gatgccatcg atctggatgg tgacggcatc gtcggcgctt cggaaacggc ctcttggctc
      421 aaagcccgca cgctatctct gcattggggt gatgaagttc ccgcgcgaga cgatgttttc
      481 gatcccgcga tcgactgggt gtatttcgac gaaaatggca atcagcagcg tgattacggg
      541 gtcgatcagg gcttcgacga ttccacgccc gcattcgggg aaccgatatt cgttcccgac
      601 gacgtgaacc gaaacggaaa gatcgatgtc ggtgagcgat tcgtcagatt gggaacctcg
      661 aagttcaaaa agtctatgtc cacatcgagc actacgccca ggtcgatcat gtgtttacgc
      721 gaggacagga cctcagctcg ctgcaacagg actacaccgg tggtgtgtat gggtatagcg
      781 atgcgatgca tgcttcaggc gtgctttcga tcatcgcggc ggatgtcccc ttggtgggaa
      841 gacgttgggt ggggatggcg cctgaagctg atctgctttt aggcttcgaa atagcacaag
      901 acagcgccaa gtcggtcacg tgggcgctcg gtgaaaaacc ggatgtcatg ctccacgagc
      961 tttccgtctg gacgggagag cctttggacg gctccgatcc ttattcgacc atggtcgatg
     1021 cctcggtcca taacgatcac gtgacccata cgtgccccac gggcaatctc ggtggttccc
     1081 gaaaacatgc atggcttacc atctcgccgg gccagaccat cgtcgcggac ttggacgttc
     1141 cctcgatcaa tgcgacgtac gtgcaaacca cgttcaacgt ccggggagaa tcaaacgcga
     1201 gcatcgtgct ggaagagccc aacggcacct cccatgacct cgtcacgggg ccatcgtaca
     1261 ccaacctgag cacaggagct ttgatgtacg tcggccacac ggcatcgagc cgaggcacgt
     1321 attattggga tgcgttgcta tatgaacacc aggcaggcaa atcgatcccc acgggcgcct
     1381 ggaaagcacg gatcaaaaac aacggcgccg ctgtcatgac ggtggatgct tattgtttcg
     1441 acgaggtatc cggatgggga ctgggggttg cgtggcaccc atccatcgcc accgatgcca
     1501 gtaccgcagg aattccttcc gtcagcgacc attgcatcgc ggtgggtgcc catacgggac
     1561 atcccatgtc cagctcgacg ccctggttca atacgcccgg agaggttggg gccgtgcgtg
     1621 attattccgg aagaggtccg cggatcgatg gtacgcaaaa gcccgatgtg cttgcaccgg
     1681 acaacccatg ggcggctgct cccaatgcgg ctgcttttgg cggaaatatc attcctcacg
     1741 gcgcggtatg gccatttgga ggaacttccg gcgccacgcc gcacgtcacc ggtgtgaccg
     1801 ctttgatggc acaggctggt gtgcgtgggc tggatgctcg acaagcgtta cgcgatagcg
     1861 ccatcgccga ttcgaccacg ggtgaagtac cgaacttcga ttatgggtgg ggacgaatgg
     1921 atgccgcggg tgccctcggc gcgctggccg cgggaaaacc acccgaagtg cagctcttcg
     1981 catcgtctct gtccgtcgct atcgacgagc ccgtcacact tactgtttcc gcagtcgacc
     2041 cggatggtcc ttccgaccag ctcgaagcca agtgggatga tggctatgat ggctcgtggg
     2101 acgtgcctta tgctccgatt gcgcctcgga cgttgtcttt cgatacgcct ggccattatc
     2161 ccacgaaggt cagagtgcga gacgtgcaag gcaggatcga tgaggcagtt gtgtggatcg
     2221 aggtagtcga gtcgactcat gagccggatg cgggagccga cgcgcaaccc gcggagggag
     2281 gagaaacaga aaccggcgcc ggtggaagtt ccggaaacac gggcggttca ccttctcacc
     2341 catcgtcttc gtccgatgat gacgatgggt gtggatgccg cgtgatcgca agtcgggatt
     2401 ccgccaaaca cgagtatggg tggctcgttg ccctcgctgt gttcgcggcc cggcgacgat
     2461 cctcgcgtgg gtgacgcagg tcttttcggg atgcgggaat gcgaatagga ggtgattcgt
     2521 agactatccg gatacggata tgaatactag gtcatctctc gatgcttccg cgcgaagaga
     2581 ggaactggcg acttggctac gatcaccccc gatatgcttc gtcttgctcg attttactca
     2641 atacccaatc gaactggccg gtcgttactt tggctttgca atactcgcag acgccagcca
     2701 tgttgatttg caggggcgcg ccgcagttgg gacaggcttt gtccgtgcgg gttggcgcgg
     2761 aacgtcctgt cccgcgaatg aatgtccagt attcggaata aggccgttct cgggatttgc
     2821 tgcctgatac aacgcgctga ccgtcttcgg tgaccgtata atcgagcccg gttgcccata
     2881 accgaaccgt gatggagtcg aaaaaccggt cggcatcgat ttttacgatt tcaagaccgg
     2941 cgatgcgcgc tcgttccgtg atgttgcgca atccctgctg tcgataggct tccacccaat
     3001 acacttgggt ttggaacaac gaatcggaaa ggtacggccg catggatgcc agatcccgag
     3061 cgctccaggc tttctggaac tcgttgaaaa tgagctgaat ccgtgcgcga aatccttccc
     3121 actgaaattg cgggtctcgc gaggtcaagg ccgccgaggc ctgctgcaac gaaggatcga
     3181 aaaccgtggg cagttgagtg ccctgctcgg ccacattggt tgtcaacatc gggcctcggt
     3241 actcggcgcg aatcaaatcg atgctccgca cggtccagtc gaatgcccca gagttgacct
     3301 gctgacggca atgagaacaa tgccccgcca cgaccacgtc gagcggagca ccacaactcg
     3361 gacacgcaag cacgatcgct tgcgcgggca cccgcgactg tggcccatgc tttctcacca
     3421 aagtccaacg ctccatcaag tatcgccctt gcggtgcgat gcctgcctgc gcccgctcgg
     3481 tcacgttggc ctcgaactcc accgccacga ccaccatcgc atcttggggc cctatccctg
     3541 aaacatgcac gattcgcatc gcccccacaa tgacgctatc cacctcgaaa gcctgacgcc
     3601 gggatagcgc cgtcatcgcc tcctgcatga gataggcgga cactcgaccg agccggttgc
     3661 cgcctcggga acgatgcacc tccgcaaaca aggcataaag aaaatcctcg aacaagaccg
     3721 t
//
LOCUS       DAOSOR010000574         3705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_24942_length_3705_cov_4.808767, whole genome shotgun sequence.
ACCESSION   DAOSOR010000574 DAOSOR010000000
VERSION     DAOSOR010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24942_length_3705_cov_4.808767"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="PK710_21840"
     CDS             <1..727
                     /locus_tag="PK710_21840"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOT12436.1"
                     /translation="FPVAALLTPVPCSAHAQSNLRWSLQVGLTAYPCAHPSLIPFVLP
                     RILRQGDMLIPPGGSNASGSIGYALAARELESQVHAGLVPDPDVIVVALGSGGTVAGL
                     LAGLEETSLRCVVHAVRVFPWPWINELSVLTLARSVARRRGTTPSWRSLRKRLWIVND
                     RLGRGYGYPTPWGERAMRMADDMGIELEATYTAKTFSHALRLAQSAQVRNILYWHTLS
                     NAKCPSLDRDIPATLESLWLPPS"
     gene            complement(764..1843)
                     /locus_tag="PK710_21845"
     CDS             complement(764..1843)
                     /locus_tag="PK710_21845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOT12437.1"
                     /translation="MTSDVNPPSQRHPHSPSLPPANERIAVVVNGNAKNVTRDVVETL
                     DQILLGGDLFVSRRLEEGPQIARTIAERGYGTVLTGGGDGTFTVMVTEVVRACRKLDK
                     KPPRFGLLKLGTGNSLAWVVGASKAKGRGLAADILRLRQEAGSRSMRLIEADDFLSPF
                     CGFGIDAVVLHHKELTKEWMLNLPVVGKYVTGEMIYAMSTFTRTFPFYMIHAMPHCRI
                     INEGADAFHVGYNGSFIGAPIPKGSVIFEGKVKLASCSTIPYYGFGFRIFPYAEERED
                     RMALRVSTVPVPTFIRNFPAIWRGEYHNPKVIEDFLVEDVTIEIDPATPFQIGGDSHG
                     TRSRVRMRLSPEPIELVDFYAPPRG"
     gene            1989..2693
                     /locus_tag="PK710_21850"
     CDS             1989..2693
                     /locus_tag="PK710_21850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12438.1"
                     /translation="MATKPKKPPTKGNKPSKTGGSSSRTGKKVVRSPKSETSSKGKSA
                     SEKPLAEAGSSKDEPRHMGIRGAAPWAARHAEKHREEARARAAEPPLPGSARATLRVP
                     TGAEEIKARIASLHNILQKINTLRKNYEQNFFAIGKLLRDIRDSKLYEVHGFGSFEAF
                     VEREVKLGKTTSIRLARVTEVFQEQAAISYGVDLVLQALATLESGKKTGGTNLQPFSV
                     GASPPPLPMRPPLPKK"
     gene            2731..>3705
                     /gene="obgE"
                     /locus_tag="PK710_21855"
     CDS             2731..>3705
                     /gene="obgE"
                     /locus_tag="PK710_21855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOT12439.1"
                     /translation="MKFVDECEVTVHAGDGGNGCIAFRREKYIPHGGPSGGDGGKGGD
                     VVFEGAGGMSTLLDLTYSHDIRAKRGQHGQGKDRYGRSGDDAVVRVPIGTRVIDTETG
                     EMLGDVLTHGQKLIVAKGGAGGRGNIHFATPYDRAPRRAEPGVPGEIRKLKLSLVVMA
                     DVGLLGFPNVGKSTLIRAVSRARPRVDDFPFTTIRPHLGIVSVGSHEQTTQFAMADIP
                     GLIPGASQGVGLGIRFLKHLERTRVLLHLITLDHEETRDPWNDYLAIRQELAAFDENL
                     ARKPELVALSKSDITEVREAYPTLADQFASHGIHLHLISAVTGENTGPL"
BASE COUNT          863 a         1103 c         1008 g          731 t
ORIGIN      
        1 atttccggta gccgcgctgc tcacacccgt tccgtgtagc gcacacgcac aatcgaatct
       61 ccgctggtcc ttgcaggtgg gtttgacagc atacccttgt gcccatcctt ccctcatccc
      121 cttcgtcctc cctcgcatcc tgcgtcaagg tgatatgctc atccctcccg gcggaagcaa
      181 cgcgtctggc tccatcggtt acgccctcgc ggcacgggaa ctcgaatctc aagtccacgc
      241 aggtttggta ccagaccccg acgtcatcgt cgtcgcactc ggttccggcg gtaccgtggc
      301 aggactgctg gctggattag aagaaacttc gcttcgttgt gtcgttcatg ccgtacgtgt
      361 gtttccctgg ccgtggatca acgaactttc cgtacttacc ctcgcacgaa gcgtggcacg
      421 tcgtcgtggc accaccccat cgtggcgctc cttacgaaaa cgtctgtgga tcgtgaacga
      481 tcggctcgga cgcggctacg gttatcccac accgtgggga gaacgagcga tgcgaatggc
      541 cgatgacatg ggcatcgaat tggaggcaac ctacacggcc aaaacttttt cgcatgcgtt
      601 gcgtctcgcg caatcggcgc aggttcggaa catcttgtat tggcatacgc tatcgaacgc
      661 aaagtgccca tctctcgata gggacatacc cgcaacactc gagtcgttgt ggcttccgcc
      721 atcgtgacga catctacgca agcgtttgtt tttacaagat attttatcca cgtggtggcg
      781 cgtaaaagtc aaccagctca atcggctctg gcgacaaacg catgcgcacg cgagagcgcg
      841 ttccgtgaga atcaccccca atttggaaag gcgtcgccgg atcgatctca atggtgacat
      901 cctcgacgag gaagtcttcg atcaccttgg ggttatggta ttcgccccgc catatcgccg
      961 gaaagttacg aatgaacgtg ggcacaggaa ccgtggagac gcgcaatgcc atgcggtctt
     1021 cacgctcttc cgcatacgga aagatgcgga atccaaagcc ataatacggg atggtggagc
     1081 aggaagcgag cttgaccttt ccctcgaaaa tgacactgcc tttgggaatg ggagccccga
     1141 tgaacgagcc gttgtacccc acgtgaaagg catcggctcc ttcattgatg atacggcaat
     1201 gcggcatggc gtgaatcatg taaaacggaa aggtgcgtgt gaaggtggac atcgcgtaga
     1261 tcatctcacc ggtgacatac ttgccgacga ccggcagatt gagcatccat tctttggtga
     1321 gttctttgtg gtgaagcacg accgcatcga tgccgaaacc acagaatggg gagaggaaat
     1381 catccgcctc gatgagccgc atggacctgc ttccggcctc ttgtcgaagg cgtaaaatat
     1441 cggcggcaag acctcgacct ttggctttgc tggcccccac gacccaggca agcgaattgc
     1501 ccgtcccaag tttcagcaat ccaaaccgcg gtggcttttt atcgagcttg cggcacgctc
     1561 gcaccacctc cgtcaccatc accgtaaacg taccgtcccc accacccgtc aatacggtcc
     1621 cataacctcg ttccgcaatc gttcgtgcga tttgaggtcc ttcttcgagt cgtcgcgata
     1681 cgaaaaggtc cccaccaagg agtatttggt ccaaggtttc gacaacatcg cgggttacgt
     1741 ttttggcgtt gccattgacc acgaccgcaa tccgctcatt ggcaggaggc aacgaggggg
     1801 aatgggggtg gcgttgcgac ggggggttga catcggacgt catggcctgc ctgtagcacg
     1861 cgacgagcat agcgggaatg caagttgctg gacgcaaggg gaacgaattg ctaaggcgag
     1921 cgtgtagacg gatggaccct tcttcgagaa ccccgcggga ggcgtaccga acgaaattgg
     1981 gtagggaaat ggctacgaaa ccgaagaagc cgccgacaaa aggcaataag ccgtcaaaaa
     2041 ctggcggttc gtcatcacgg acggggaaaa aagtggttcg ctcccccaag tccgaaacct
     2101 cgtcgaaagg gaaatcagcc tcggaaaagc ccttggccga agccggttcg agcaaggacg
     2161 aacctcgtca catgggaatc cgaggagccg cgccctgggc tgcacggcac gccgaaaaac
     2221 atagggagga agcccgcgcc cgcgcggccg aaccacccct tcccggctcg gcaagagcta
     2281 ccctccgcgt gcctaccggt gccgaggaaa tcaaagcgcg aattgcctcg cttcacaata
     2341 tccttcagaa aatcaatacg ttacgaaaaa attacgaaca gaattttttc gcgatcggaa
     2401 aattattacg ggatatccgc gacagcaaac tctacgaagt acatggcttc ggctccttcg
     2461 aagcgttcgt cgaacgagaa gtgaaactcg gaaaaacaac ttccatccgt ctcgcccgtg
     2521 tcaccgaggt gttccaagaa caagcggcca tcagctatgg cgtggatctg gtgttgcaag
     2581 cactggcaac cctcgaatcg ggcaaaaaaa ccggcggtac taacctccaa cccttctccg
     2641 tcggcgcaag cccccccccg ctgcccatgc gcccaccatt gcccaaaaaa tagcatccaa
     2701 taacactcac ctacctctgc actcaacgtc atgaagttcg tggacgaatg cgaagtcaca
     2761 gtacacgccg gcgatggtgg caacggttgc attgcctttc gacgggaaaa gtatattcct
     2821 cacggtgggc cttccggcgg tgatggggga aaaggaggcg acgtcgtttt cgaaggcgcg
     2881 ggcggaatgt ccaccctcct cgatctcacc tactcccatg atatccgcgc caaacggggc
     2941 cagcacgggc aaggaaagga ccgttacgga cgctccggag atgatgctgt cgtgcgcgtt
     3001 cccatcggta cacgagtcat cgataccgaa accggcgaga tgctcggtga cgtcttgacg
     3061 cacggccaga aactcatcgt cgcaaaaggc ggggccggcg gacgcggcaa catccacttc
     3121 gcaacaccgt atgatcgcgc gccacgacgg gcggagccgg gcgtaccggg cgaaatccga
     3181 aaactgaaac ttagtctcgt ggtcatggcc gatgtcggct tgctgggctt tcccaatgta
     3241 gggaaaagca cgttgatccg agcggtttca agagcccgcc cccgcgtcga tgacttcccg
     3301 ttcaccacaa ttcgtccgca cctcggaatc gtatccgtcg gcagtcacga gcaaaccacg
     3361 caatttgcca tggccgatat cccaggattg attcctggcg ccagccaagg cgtcggcctt
     3421 ggtatccgtt ttctcaaaca cctggagcgc acacgcgtgc tgctgcacct catcaccctc
     3481 gatcatgagg aaacgcgcga cccctggaac gattacctcg ccattcgaca agaactcgcg
     3541 gcgttcgatg aaaacctcgc tcgtaaacca gaactggtcg ccttgtcgaa aagcgatatc
     3601 accgaggtac gagaagccta tcccacccta gcggaccagt tcgcaagcca tggcattcat
     3661 ttgcatttga tcagcgccgt gactggagaa aacacagggc cactc
//
LOCUS       DAOSOR010000575         3702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_24974_length_3702_cov_3.539896, whole genome shotgun sequence.
ACCESSION   DAOSOR010000575 DAOSOR010000000
VERSION     DAOSOR010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24974_length_3702_cov_3.539896"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="PK710_21860"
     CDS             <1..1083
                     /locus_tag="PK710_21860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12440.1"
                     /translation="VWLATAPACSSSSGHSEEPSPTLVTVSPYTFGQGVVCGDFPGAW
                     KSYVATLTDVTDPSRPFILASSNPVPCSMPVSFSWVVPGHEYVAEIDGYEQRDLVSFG
                     GESSGSRHVIDPATGLEMSPRWQTTCGKTTEHAEAETTMAYLQRNVVVGDCRPLSEIK
                     PGTSPTSLVLDLSTVRGALVCGQQPGQVSRFRVSPDDPALSALDADCDEQLTFSPLDP
                     KKRYRFHIEGFESEASSPTWGTWCEGRTEAGVSLPTACDPLVTQGALQVDMPQLLASS
                     PYACSPEDIVSYRALLLGYSLASEKRNCSSPVVFPGLEPTTYQVLIDAFDSSGRERLN
                     AFCEGQVQPGQIVDVHCQIKAAEGVE"
     gene            1184..2008
                     /locus_tag="PK710_21865"
     CDS             1184..2008
                     /locus_tag="PK710_21865"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HOT12441.1"
                     /translation="MRLWCRSSMACLGLWLTWSAVAWADPSVEASVDPPDSAQPSEST
                     EPREPAWCASELEVWQDEICSYIPENLPQDRPTTLVIYLHGVIQPGTTWQYGPQRGMV
                     RAANKHGFAVIMPRGRRGIGPGSMRDWWTWPTSARAQTQVEQAILQQWAQARAWLEQR
                     LGKPFEKVYVVGFSNGAYYAASLALRGVLSVNGYVIVAGGSAQAASQGGAQATHRPPI
                     YIGYGHRDSVGQDCRSLGQALARLRWKHRIVGRPRVGHLMTDSQIEEAMEFFRTIP"
     gene            2005..3081
                     /locus_tag="PK710_21870"
     CDS             2005..3081
                     /locus_tag="PK710_21870"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOT12442.1"
                     /translation="MNTAKVFLRRVCAAESPEQWGQAAQDVLEASQTLEFIRKRDLIA
                     VKLHVGEPGTTTFLPAEVAAGVVRCIRAREARPFLTDSCVLYSGPRSNGAEHAEVAAR
                     HGFCLERTGAVFVPADGLEGNQERIVGIDGNHFREVGVAALIADADGLVVLSHATGHL
                     SAGYGGTIKNLGMGCASRKGKLLQHSDTKPRIRRDACTACGACVEACPERAIMQDAQG
                     IAAIDDPLCIGCGECIAQCRSDAVRFRWDSASHAMQEKMAEHALGVVRHAKGRLTFLL
                     GLVNLTQDCDCMGCRSPIVARDIGFAVSSDPVALDQAAFELVRAAENQTLDKLSYPLL
                     DGAHQVEYAERLGLGTRRFELIEV"
     gene            3299..>3702
                     /locus_tag="PK710_21875"
     CDS             3299..>3702
                     /locus_tag="PK710_21875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12443.1"
                     /translation="MRTRLILISSFGIALLSSRGVLAADINADPSNYKAALPTLKPGD
                     TLHLAGGTYPKLLVINNLKGTESQPIVIQGPSSGAPAVFTADPGPCCNTIEITNSEYV
                     VLRYLTVDGKGVDGAFGLSAKGGDNNLVHHITV"
BASE COUNT          742 a         1066 c         1058 g          836 t
ORIGIN      
        1 gtttggctgg ccactgcgcc cgcttgttcc tcttccagtg gacacagcga ggaaccctct
       61 cccacgttgg tcacggtatc gccctacacg ttcggtcaag gcgtcgtctg cggtgacttt
      121 ccaggagcat ggaaatccta tgtcgccacg ctcaccgatg tcactgaccc gtcgcggcct
      181 ttcatattgg cttcctccaa tcccgtgcct tgcagcatgc ccgtgtcgtt ttcctgggtc
      241 gttccaggtc acgagtatgt cgcagaaatc gatgggtatg agcaaaggga cctggtgtcc
      301 ttcgggggtg agtcttcggg aagccgtcat gtgatcgatc ctgcgacggg tctggaaatg
      361 agcccccgct ggcaaacgac ctgcggaaaa acgacggaac acgcggaagc agaaacgacg
      421 atggcctact tgcagcgaaa tgtcgtcgtt ggggattgtc gccccctttc ggaaatcaaa
      481 ccaggaacga gtccaacgag cctcgtgctc gacctatcca cggttcgggg agcgctggtt
      541 tgcggtcaac aacccggcca ggtgagtcgg tttcgtgtct cccccgacga cccggccttg
      601 tctgccctcg atgcggattg tgacgaacaa ctgacgttct cacccctcga ccccaaaaaa
      661 cgatatcgat tccatatcga aggcttcgaa tcggaagcct cttcccccac atggggtaca
      721 tggtgcgaag ggcgtacgga agctggggtt tcgctgccca ccgcatgcga tccgttggtg
      781 actcaaggcg ctttgcaagt cgatatgccc caattgctcg cctcctctcc ctatgcttgc
      841 tccccggagg atatcgtttc ctatcgcgcc cttttgctcg gctactccct tgcctcggaa
      901 aaacgaaatt gctcgtctcc cgtcgttttt cccggactag aacccacgac ataccaggtg
      961 ctcatcgacg ctttcgactc ttccgggcgt gagcgattga acgccttttg tgaaggtcag
     1021 gtccaaccag gccaaatcgt cgatgttcat tgtcagatca aagctgctga gggcgtggaa
     1081 tagaaaagag caataatatc actgtattac gcttgtgtcg gcgatcctat gccctgtttc
     1141 gaatgcggca ttccttttgg cttcgtgcta ccaccgcaac gtcatgcgtt tatggtgccg
     1201 ttcgtcgatg gcatgccttg gcctgtggct gacttggtct gccgtcgcat gggccgaccc
     1261 ttcggtggaa gcgtcggtgg acccgccgga tagcgctcag ccatcggaat ccaccgaacc
     1321 gagggagcct gcgtggtgtg cgtctgaact cgaggtgtgg caagacgaga tctgctcgta
     1381 tatccccgag aatttgccgc aggacaggcc gactaccctg gttatttatc ttcacggtgt
     1441 cattcaacct ggcaccacgt ggcagtacgg accgcaacgg ggcatggtac gagccgccaa
     1501 caaacatggt ttcgcagtca tcatgccgcg tggacgaagg ggaatcggtc ccggttcgat
     1561 gcgggattgg tggacctggc ccacgtccgc gcgcgcgcaa acacaggtgg agcaggcaat
     1621 tttgcagcag tgggcgcaag cccgtgcgtg gctcgaacag cgcctcggca agcctttcga
     1681 aaaagtatat gtggttggtt tttccaacgg tgcctactac gccgcttctc tggctcttcg
     1741 cggcgtattg tccgtaaacg gatatgtcat tgtggcggga gggtccgccc aagccgcttc
     1801 ccagggggga gcgcaggcca cccaccgccc acccatctac atcggatatg gtcatcgcga
     1861 ctcggtggga caggattgta gaagcctggg acaggctctt gcacgccttc gatggaaaca
     1921 tcgtatcgtc ggacgaccac gagtgggtca tctcatgacc gactcgcaga tcgaagaagc
     1981 catggagttt tttcgaacca tcccatgaat accgccaagg tttttcttcg acgcgtttgc
     2041 gcggctgagt cgcctgaaca atggggccag gcggcccagg atgtgctaga agcctcacaa
     2101 acccttgaat tcattagaaa acgggattta attgccgtca aactccacgt gggggagccg
     2161 ggaacgacga cctttcttcc tgccgaggtc gcggctggcg tcgtccgctg cattcgcgcc
     2221 cgggaggctc gtccctttct gaccgattcc tgcgtgttgt acagcgggcc ccgttccaac
     2281 ggtgccgagc atgcggaagt tgccgctcga catggcttct gcttggaacg caccggtgcc
     2341 gtttttgtcc ccgccgacgg gttggaagga aatcaagaac gaatcgttgg catcgatggc
     2401 aatcacttcc gcgaagtcgg tgtagccgcc ctcattgccg atgccgatgg gcttgtcgtc
     2461 ctttcccatg caaccggcca tctttccgct ggatatggcg gaaccatcaa aaatctgggc
     2521 atgggctgcg cctctcgaaa aggcaagctc ctacagcata gcgataccaa accgcgtatt
     2581 cgtcgtgatg cctgcacggc ctgtggcgct tgtgtcgagg cctgtcccga acgagccatt
     2641 atgcaagatg cgcagggaat tgctgccatt gacgatccgc tttgtatcgg ttgcggagaa
     2701 tgcatcgcgc agtgtcgaag cgatgccgtt cgatttcgtt gggatagtgc atcccatgcc
     2761 atgcaggaaa aaatggccga acatgccctt ggcgtggtgc gtcacgccaa aggtcgtctg
     2821 accttcttgc tcggcttggt gaacttgacc caggattgtg attgcatggg atgccgatcc
     2881 cccatcgtgg caagggacat tggctttgcc gtttcttccg accccgtcgc gctcgatcaa
     2941 gctgctttcg aattggtccg tgcagcggag aaccaaacct tggacaagct ttcctacccg
     3001 ttgctcgatg gtgcgcatca agtggaatat gcggagaggc ttggtttggg aacacgtcga
     3061 ttcgagctga tcgaagtatg atcgaggatg cggcccgttt ggtaatcatc ggactcggtc
     3121 gcagcttcgg gtgcgacacg atgggatcgc gaaaagctcg atacggcatt ggagaggaca
     3181 tgaatgacgc agattggtag cggcaatgcg taaattcatg cgttttcgag ggtgacggat
     3241 ttggcctgag atatgctggg gtagctgtca tcgaacgccc attgtggaag gaaccaacat
     3301 gcgaacacgg ctcatcctca tctcatcttt tggtatcgcg ttactatcct cgcgcggcgt
     3361 cctggcggcg gatatcaacg cggatccgag caattacaag gccgccttgc cgacactgaa
     3421 acctggcgac acgcttcacc ttgcgggcgg cacctatccc aaattgctcg tgatcaacaa
     3481 tctcaaagga acggaaagcc agccgatcgt cattcaaggt ccgtcctctg gcgctcccgc
     3541 cgtgttcacc gcggacccgg ggccctgctg caacaccatc gaaatcacca actccgaata
     3601 cgtcgttttg cgctatctta ccgtcgacgg caaaggagtg gacggcgctt ttggactgag
     3661 cgccaaaggg ggcgacaaca atctcgtcca tcatatcacc gt
//
LOCUS       DAOSOR010000576         3696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_25030_length_3696_cov_4.823675, whole genome shotgun sequence.
ACCESSION   DAOSOR010000576 DAOSOR010000000
VERSION     DAOSOR010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25030_length_3696_cov_4.823675"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1109)
                     /locus_tag="PK710_21880"
     CDS             complement(120..1109)
                     /locus_tag="PK710_21880"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOT12444.1"
                     /translation="MGRWLVFFSTGLFLAACSDDSESSQDWWPPSDGATEQDASVDDV
                     ITVDDASGKDVAQQDQKTEDVTSPSDAEQEADAPPVVTTVDVSHRREVRGVWIATVSR
                     INWPTSTKASEQKAQMEAILDTVEKAGFNTVFFQVRPEADALYASDLEPWSRYLTGTQ
                     GVDPGYDPLTFTLESCHRRGLELHAWLNPYRARAGNVGASAPNHVINQMPQAVVSYGN
                     LRWLDPGHPDAFNHTLAVIEDLLERYDFDGLHFDDYFYPYPDTGLTFDDNATFAAYGG
                     GKSRADWRRDNVNRMVAAVGERVKAMRDDVRWGISPFGVYRPGMPAGVTGLPP"
     gene            complement(1182..3587)
                     /gene="lon"
                     /locus_tag="PK710_21885"
     CDS             complement(1182..3587)
                     /gene="lon"
                     /locus_tag="PK710_21885"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOT12445.1"
                     /translation="MSEKKNPHRSEDEVTFGDRLPVLPIRNAVLFPGAVAPFDVGREK
                     SVALVEEMDSFSSPVIAIFAQRDPATDDPGFDDLYPVGCAARVLKALKHSSGNYSLIL
                     QGLTRIRLTSMPQTSPYLIAKVQKLEPPATDDVEIEALAMSLREIAKQVIQLMPELPR
                     EASALIDSIQEPGALSDLVAANLDAPVEEKAQLIETVDVKERIRKVLRLLTRQLEVLK
                     MRDRINSQIKEEMGKNQREYVLRQQLKAIKEELGEDEGDQGDLDGLEERISKAELPQE
                     ADQVARKQLKRLRAMQVGSAEYTVVRTYLDWILDIPWATSTQDNMDILKVREVLDEDH
                     YGLDKVKKRIVEYLAVRKLKKDKKGPILCLIGPPGVGKTSLGKSVARALGRKFHRISL
                     GGVHDEAAIRGHRRTYVGALPGQIIQGMKKAGTINPVFMMDEVDKIGHDFRGDPSAAL
                     LEVLDPEQNNSFADHYLEIPYDLSHVMFIATANVPDPIPPPLRDRMEILDLPGYTRRE
                     KLAIARRHLIPKQLEEHGLAEEQVNITDAAVEEIIDHWTREAGVRNLERSIASVIRGV
                     AVKVAEGDDTPRTIETEDQLREYLGPIRFTSEVAERTEETGVATGLAWTSVGGEILFI
                     EATKMFGTGKLQLTGQLGDVMKESAQAALSYVRTNAERYGINRDFLEKSDLHIHIPAG
                     AMPKDGPSAGVTMFTALVSMLTGIRVRHDVAMTGEITLRGRVLPVGGIKEKVLAAHRA
                     GIRRLLLPERCKADLEEVPKEIKDELEFVFVQRMDQVLENALETMPTPLPADVQNAPP
                     PAN"
BASE COUNT          756 a         1097 c         1006 g          837 t
ORIGIN      
        1 gccttcgtgc aggtgctgct gctgagccgc accgaggagc cgctgggcag ccgcaaatac
       61 tggccgatct acgaggccgc ggccgaggtg ggcatgccgg tgggcgtgca cgccttcggc
      121 tatggcggca accccgtcac ccccgcgggc atgcccggcc ggtaaacccc aaagggagat
      181 atcccccaac ggacatcgtc acgcatcgcc ttcacccgct cccccaccgc cgccaccatc
      241 cgattgacat tgtcccgacg ccaatccgca cgtgattttc cccctccata cgccgcaaaa
      301 gtagcgttgt catcgaaggt caatccagta tccggatagg gatagaagta atcgtcgaaa
      361 tgaagtccat cgaaatcgta gcgctcgagt aagtcctcga tgacagccag cgtgtggttg
      421 aacgcatcag gatgacctgg atcgagccat cgcaagttcc cgtaagacac gaccgcttgc
      481 ggcatctggt tgatgacgtg attcggcgcg ctcgccccca cattgccagc tctggcccgg
      541 taggggttga gccaggcgtg cagttcgagc cctcgacgat ggcaggactc gagcgtgaag
      601 gtcaatggat catagccggg gtccactcct tgggttcccg tcaagtaccg actccaaggc
      661 tcgagatccg aggcatacaa agcatccgcc tcagggcgta cttgaaagaa aaccgtattg
      721 aatccagcct tttcgaccgt atcgaggatg gcctccatct gcgccttctg ctcggaagct
      781 ttggtgctcg tcggccagtt gatacgcgat accgtcgcaa tccatactcc ccgcacctcg
      841 cgacgatggg atacgtccac cgtggtgacg acgggtggag catcagcttc ttgctcggca
      901 tccgacgggc tcgtcacatc ttcggttttt tgatcttgct gcgcgacatc cttgcccgat
      961 gcatcatcga ccgtgatgac atcgtccacc gaggcatcct gctcggtggc tccatcggaa
     1021 gggggccacc aatcctgact cgactcggag tcatcggaac aggcggcgag aaaaagaccc
     1081 gtggagaaaa aaactagcca acgacccatg agtcgcgctt agcatacaag gcgcactccg
     1141 accacgagcc agaggaaagg gagaagaaat tagcgggctg atcaatttgc cgggggaggc
     1201 gcgttttgca cgtcggcggg tagcggcgtg ggcatggttt ccaaagcatt ttccagcact
     1261 tgatccatgc gctgcacaaa tacgaattcg agttcgtctt tgatctcctt gggaacctct
     1321 tcgaggtccg ctttgcaacg ctccgggagc aaaagacgcc gaattcccgc gcgatgggct
     1381 gccaggactt tttccttgat gccccccacc ggcaacaccc gccctcgaag cgtgatctca
     1441 ccggtcatgg ccacatcatg acgaacgcgg atcccggtca gcatggaaac gagcgccgtg
     1501 aacatcgtca cgccagcgct ggggccatcc ttgggcatcg cgcccgcagg aatatgaata
     1561 tgcaaatcgc ttttttcgag aaaatcccgg ttgatgccat aacgctcggc attggtcctc
     1621 acgtaggaca aggccgcttg cgcggactct ttcatcacat caccgagttg acccgtgagc
     1681 tgaagcttgc ccgtaccaaa catcttcgtg gcttcgatga ataggatttc ccctcccacg
     1741 ctggtccaag ccagccccgt agccacaccg gtttcctccg tacgttccgc cacctcactg
     1801 gtgaagcgaa tcggtccaag atactcgcgc aactggtctt ccgtctcaat ggtgcgaggc
     1861 gtgtcgtcgc cctccgctac tttcaccgcc acgccacgga tgacgctcgc aatcgatcgt
     1921 tcgagattgc gaacaccagc ttctctcgtc caatggtcga tgatttcttc gaccgcggca
     1981 tcggtgatgt tgacttgttc ttccgctagg ccgtgctctt cgagttgctt cgggatcaag
     2041 tgacgacgtg cgatggcgag tttttcacga cgggtatatc ctggcagatc gaggatttcc
     2101 atgcggtccc gcaggggcgg aggaatcggg tccggaacgt tggcggtggc aatgaacatg
     2161 acatgcgaca ggtcataagg gatctcgagg tagtggtccg caaaggagtt gttttgctcc
     2221 ggatcgagca cttcgagcag cgctgccgac ggatctccgc ggaagtcatg gccgatttta
     2281 tcgacctcat ccatcatgaa cacgggattg atcgtgcccg cttttttcat tccttgaatg
     2341 atctgcccgg gaagagcccc cacgtaggtt cgtcgatggc cccggatggc cgcttcatca
     2401 tgcacacccc ccaaagagat gcggtggaat ttccgaccaa gcgcacgcgc cacgcttttt
     2461 ccaagcgatg ttttgcccac gccaggaggc ccgatgaggc acaaaattgg gcccttttta
     2521 tcttttttga gctttcgaac cgccaggtat tccacgatgc gttttttcac tttgtccaag
     2581 ccgtaatggt cctcgtcgag cacctctcgt accttgagga tatccatgtt gtcttgcgtg
     2641 gatgtggccc aggggatatc gaggatccaa tcgagatacg ttcgcaccac cgtgtattcg
     2701 gctgacccca cctgcatagc tcgaagacgt ttgagctgtt tgcgggcgac ttgatcggct
     2761 tcttgaggaa gctccgcttt ggaaatacgc tcttcgagtc catccagatc tccctggtcg
     2821 ccttcgtctt cgcccagttc ttctttgatg gccttgagtt gttgacgcag cacatattcg
     2881 cgctggtttt tccccatttc ctctttgatt tgggagttga tgcggtcgcg catcttgagc
     2941 acttctagct ggcgtgtaag caaacgaagg acttttcgaa tacgttcctt gacgtccacc
     3001 gtctcgatga gttgcgcctt ttcttccacc ggcgcgtcga ggttggcggc caccaaatcc
     3061 gacaacgctc cgggctcctg aatggaatcg atgagagccg atgcctcccg aggaagttcg
     3121 ggcatgagtt gaatgacctg ttttgcaatc tctcgaagac tcatcgccag cgcttcgatc
     3181 tccacatcat cggtcgcagg cggttcgagc ttctgcacct ttgcaatgag atacggtgac
     3241 gtttgcggca tggatgtcag ccgaatccgt gtcaatccct gcaaaatcag ggagtaattg
     3301 cctgaactat gtttcaaggc tttcagcact cgcgccgcac accccaccgg gtatagatca
     3361 tcgaaaccag gatcgtctgt cgctggatcc cgttgcgcaa aaatcgcaat cacaggactg
     3421 gaaaagctat ccatctcctc gacgagggcg acagactttt ctctgcccac gtcgaagggc
     3481 gccacagcgc caggaaaaag cacggcattt cgaatcggca atacgggaag ccgatctccg
     3541 aaagtaactt cgtcttctga acgatgcggg ttcttttttt ccgacatgat gtttccttgg
     3601 atgcccggct cgcgtggctc atcgcttcac gatgctcaac gttggttgcg gaccagcatg
     3661 tctggagcaa gctaatcacg acaggcggtg ggggct
//
LOCUS       DAOSOR010000577         3690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_25091_length_3690_cov_4.337827, whole genome shotgun sequence.
ACCESSION   DAOSOR010000577 DAOSOR010000000
VERSION     DAOSOR010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25091_length_3690_cov_4.337827"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..774
                     /locus_tag="PK710_21890"
     CDS             133..774
                     /locus_tag="PK710_21890"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT12446.1"
                     /translation="MAQRPKPEVREAIVHAAALAFCEHGFEDATLIDIVNRAGTSIGN
                     LYKYYPNKNELFSAFLPPSFPKELTSRLSQQVRALHAETNAFELDHQHPYRQASERLL
                     AFSIAHRERVVFLLLRAKGSKYEPFAADIVRRLVGLAVGYAKKAHPTLVFTPARRRAL
                     VRIYRGFVLALGAILVEERTEKACREAVALLTRYHLSGLAALFAHTASRSPSP"
     gene            771..2165
                     /locus_tag="PK710_21895"
     CDS             771..2165
                     /locus_tag="PK710_21895"
                     /inference="COORDINATES: protein motif:HMM:NF039677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6178 family protein"
                     /protein_id="HOT12447.1"
                     /translation="MTLSRYSSRELIRARSSLHALLGLPDLAQTIKSLPVEAFSALVR
                     SIGLEDAGEIVALATTDQLVQAFDEDLFVSEKSGERERFDVKRFALWLEVLLEAGEEL
                     TADRISELDESFVAHALSGLILVLEEDALRERLDESGERQGRLVDKAIENALLEEIDG
                     YLLVAKQQQGWDSVLALVLALDRNHRPLLVRLLDRLVRIGSHLLDDLDELSTVLSEGE
                     SLAEDVEAAREERRSRQGYVEPRAARAFLLLARRPMDISDRRGERDPLTKAYFRELMR
                     FSSPGGSAPTQAASAFLPSVDPPRLAQATDASFVNDASDLTAMGVFVRSLRELNDTQA
                     SVFSERMDEFAYLVNVLVAGFDEEGERLSRKKSVRAALATVCFGAVLAYRASQSPRNA
                     GALPTQADYLKIIRKWSADWLFRLASSALSSGAAPDVKGAKKRGFLMSGRQLDKALDT
                     ATPSSSTPLKTSSS"
     gene            complement(2146..>3690)
                     /locus_tag="PK710_21900"
     CDS             complement(2146..>3690)
                     /locus_tag="PK710_21900"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12448.1"
                     /translation="RANDFERRTEEGQLKGKLGYMSPEQVTGQAIDSRSDLFTLGIVM
                     AELLVARPLFGIGNELDVLMRIRDADLSTFERYGARLPSELRDVVRKALAQKPDDRFS
                     NATEFAEAIEDFLRQSRTPTGAHRLVAWLHHASLIPSTKSSDSALAIGRTRHSTPRAF
                     AAVVPIKNVTPSVPTVPMKPPMGAETVVPSDYRVVSGQQSWGPVSYAKMVELFATGRA
                     QTDSLVARGAGPFRPARDIPELARLLLSAALSAPPEAPEHALERWELDRASHVHKLFS
                     IVSSKQTGLLIAAQGNRMKRFFLSEGAFTFATSTDKAELLGQQLIARGQAMQMEVDMA
                     LALCPRYGGRLGDALVGLGVLRPVELFRALVAQIHDRLVDLVSWRSGSLQFIRGVRAE
                     EEAILHGVAPVETLAQGVINGYDQEELSQILRPYMQSEIRSAPPGEWTASALRLKHKH
                     LHIIEKVSKPTVLGTLIDQTLTSDTTTKDDVLRAVFIGLSAGLLLATGWPPTPRLPSI
                     PTKKMF"
BASE COUNT          750 a         1100 c         1047 g          793 t
ORIGIN      
        1 gggccgggga tgggggggca gcccgggccg gggaagggag cctctttccc gtgacacgcg
       61 gattacaaac acgttctagc cgcaccccaa tcccttgcct ccttcctcaa aagcgaatat
      121 acaattctct ttatggcaca acgaccaaaa ccagaggtcc gcgaagcgat tgtccatgcc
      181 gcggctctcg cgttttgcga gcatggcttc gaagacgcaa cgctcattga catcgtgaat
      241 cgtgcaggca cctccattgg taacctgtac aaatactacc ccaataaaaa tgagctattt
      301 tcagcgtttc taccgcctag ctttccgaag gaactcacct ctcggctcag ccaacaagtc
      361 cgcgcgttgc atgccgaaac gaatgccttc gaactcgacc accaacaccc gtaccgacag
      421 gcttccgaaa gactattggc tttttccatc gcccatcgag agcgtgtcgt attcttgctc
      481 ctgcgcgcca aagggtccaa gtacgaaccc ttcgctgccg acatcgtgcg acggctggtt
      541 gggttggctg ttggctatgc caagaaggcc catccaacgc tggtgttcac gccggcccga
      601 cgtcgtgcgc tcgtgcggat ctatcgggga ttcgttctgg cccttggcgc gatcttggtc
      661 gaagagcgga cggaaaaggc atgccgtgag gccgtcgccc tactaacccg atatcacctc
      721 agtgggctcg ccgccctctt tgcccacaca gcctctcgga gtccatcgcc atgacattgt
      781 ccagatattc ttcgcgggaa ctcatccgtg cacgatcgtc gttgcatgct ctccttgggt
      841 tgccggatct tgcacagacc ataaaaagtc tccccgttga ggcgttttcc gctctcgttc
      901 gaagcattgg gctcgaggac gcgggggaga tcgtcgctct ggcaacgacc gaccaactcg
      961 tgcaagcctt cgatgaagat ctattcgtca gcgaaaaatc gggagagcgt gagcgtttcg
     1021 atgtgaaacg gttcgccctt tggctcgagg tgctattgga agcaggcgag gagttgaccg
     1081 ccgatcggat atccgaactc gatgagtcat tcgtggccca cgccctatcg ggtctcatct
     1141 tggtgttgga ggaggatgcc ttgcgtgaac ggctcgacga gagcggtgaa cgtcagggcc
     1201 gtttggtcga taaagccatt gaaaacgctc tgttagaaga gatcgacggc tatcttctcg
     1261 tggccaaaca gcagcaggga tgggattcgg tcctggcact cgtgctcgcg ctcgaccgca
     1321 accatcgccc gttgctcgtc cgtttgctcg atcggttggt gcgaattggg agccatcttc
     1381 tcgatgactt ggacgagcta tcgacggtgc tttcggaagg cgaatctctg gcggaagatg
     1441 tggaagcagc gcgcgaggag cgtcgcagca ggcaagggta tgtggagcca cgagccgcgc
     1501 gtgccttttt gttgttggcg cgacgaccga tggatatttc cgatcggagg ggggagcgag
     1561 acccgttgac gaaggcatat tttcgggaac ttatgcgctt ttcttctcct gggggctcag
     1621 cgcccacgca agccgcgtcg gctttcctgc cttcggtcga tccgccacgg ttggcgcagg
     1681 caacggatgc ttctttcgtg aacgatgcct cggacctgac ggcaatggga gtattcgtgc
     1741 ggtcgcttcg cgaattgaac gacacccagg cttcggtgtt ttccgaacga atggatgaat
     1801 ttgcctattt ggtcaacgtg cttgtggctg gtttcgacga ggagggggag cgattatcac
     1861 gaaaaaagtc ggtgcgtgcg gcgcttgcca cggtttgttt cggggcggtg ctcgcgtatc
     1921 gggcgagtca aagcccgcgc aacgccggag cgttgccgac gcaggcggat tacttgaaaa
     1981 ttattcgcaa gtggtcggcg gactggctgt ttcgtcttgc ctccagcgcc ttgtcgtccg
     2041 gtgcggcacc cgatgtcaaa ggtgcgaaga aacgcggatt tctaatgtcc gggcggcaac
     2101 ttgacaaagc tctcgataca gccaccccgt cttcctccac ccccctcaaa acatcttctt
     2161 cgtaggaatc gaaggcaacc gcggtgtcgg cggccacccc gtcgccaaca gcaatcccgc
     2221 gctcaatcca atgaacaccg cacgcaatac atcgtctttc gtcgtcgtgt ccgaagtcaa
     2281 cgtttgatcg atcaacgtgc ccagcacggt aggtttggat actttttcga tgatatgaag
     2341 atgcttgtgt ttgagacgca acgcgctcgc tgtccattcc ccaggcggcg cgcttcgtat
     2401 ctcgctttgc atatatggac gcaaaatctg cgataattct tcttgatcgt agccgttgat
     2461 gacgccttgg gcgagcgtct ccaccggcgc aaccccatga agaatcgcct cttcttccgc
     2521 acgaacaccc cgaatgaact gcaacgatcc ggaacgccag cttacgagat ccacgagcct
     2581 atcgtgtatt tgcgccacca aggcgcgaaa tagctccaca gggcgcaaca cgccgagccc
     2641 gacgagagca tcgcccaacc ggcccccata gcgaggacac aacgcaagag ccatatccac
     2701 ttccatctgc atcgcttgtc ctcgtgcgat gagctgctgt ccgagcagtt cagccttgtc
     2761 ggtggaggtg gcgaacgtga aggcgccttc cgacaggaaa aaccgcttca tccggtttcc
     2821 ttgggcggcg atgagaaggc ccgtttgttt gctggacacg atagaaaata gcttgtgaac
     2881 atggctcgcg cgatccaatt cccagcgttc caaagcgtgt tccggtgcct cgggaggtgc
     2941 cgataacgcg gcggaaagca gcaaacgggc gagttccggg atgtctcgcg cgggccgaaa
     3001 aggaccggcg ccgcgggcca cgagactatc ggtttgcgct cgacccgtcg cgaataactc
     3061 gaccattttc gcataggaaa ccggccccca cgattgctgg cccgacacca cgcggtagtc
     3121 ggaaggcacc acggtttccg cccccatcgg cggcttcatc ggaaccgtcg gtacggaagg
     3181 agtgacattt tttatcggca ccacggccgc aaacgctcgt ggcgtcgaat gccgcgttcg
     3241 tccgatcgcc aacgcggagt cgctcgactt ggtcgatggg atcaacgacg cgtgatgcaa
     3301 ccacgccacc agacgatgcg ctcctgtcgg cgttcggctt tgtcgaagaa aatcctcgat
     3361 ggcttccgcg aattcggtag cattggagaa tcgatcgtcg ggtttttgcg ccaacgcttt
     3421 tcggacgaca tcccgcagct ccgacggcaa acgcgcgcca tacctttcga aggtcgaaag
     3481 atccgcatcc cgaatccgca tcagcacatc caattcgttg cctatcccaa acagcggcct
     3541 cgccaccagc aattccgcca tcacgatgcc caacgtgaac aagtcgcttc gcgaatcaat
     3601 cgcctgaccg gttacctgct cgggcgacat atacccgagc ttgcccttga gctgcccttc
     3661 ttccgtgcga cgctcgaagt cgttggcgcg
//
LOCUS       DAOSOR010000578         3683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_25154_length_3683_cov_4.695976, whole genome shotgun sequence.
ACCESSION   DAOSOR010000578 DAOSOR010000000
VERSION     DAOSOR010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25154_length_3683_cov_4.695976"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PK710_21905"
     CDS             <1..482
                     /locus_tag="PK710_21905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibro-slime domain-containing protein"
                     /protein_id="HOT12449.1"
                     /translation="NQNNFDQWYRYTENVNKVYQLFLKFAPHEGVYTFMSSAFFPLDN
                     KGWGNTPGYDHNFGFTTELHTRFKYEGGEIFRFTGDDDVWVFINGELAVDLGGLHPPV
                     SKTIELDAIAADFGLVKGKAYSIELFHAERRPEGSNFRVDTNLDFLDCGSVVPDRPK"
     gene            957..2186
                     /locus_tag="PK710_21910"
     CDS             957..2186
                     /locus_tag="PK710_21910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HOT12450.1"
                     /translation="MKILHTSDWHLGRVLYGKKRYKEFEDFLDWLLKTVIDEKVDVLL
                     VAGDIFDTSTPSNRAQELYYRFLHQLIDSPCRHAIFIAGNHDSASFLDAPKQLLEALH
                     IHVVGKKRDPVDDEVLVLRDRQGAPELIVAAVPYLRDSDIRDAEAGESVEDKGKKLIE
                     GIHRHYAEVAKLAEKTRQQWGEDIPIVGMGHLFTSGGRIVDGDGVRELYVGSLAHVDA
                     SRFPSCFDYLALGHLHVAQKVHGMETARYSGSPLPMGFGEAHQKKKVYLVEFEKRKAS
                     VESLDVPVSQELERIQGNWDVISARIRTLAATASKKWLEVIYDGDEVIGNLRERLEAL
                     VEDSPMEVLRIKNNRIIEQVMKRCHDDESLGELKADEVFRRCLEAHDVPETQRPGLIQ
                     AYQEVLQSLLEEDVRVQ"
     gene            2198..>3683
                     /locus_tag="PK710_21915"
     CDS             2198..>3683
                     /locus_tag="PK710_21915"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT12451.1"
                     /translation="MRILQVRFKNLNSLEGEWQIDLTHPSFTSESIFAITGPTGAGKS
                     TILDAICLALYGRTPRLEKVTKSSNEIMSRQAEDCFAEVVFETDNGRFRCHWSQRRSR
                     GKAKNDWQPAKHELANADSGVILEAQIRKVAAAIEAATGMDYYRFTRSMLLAQGQFAA
                     FLQASPDDRAPILEQITGTEIYSDISIRVHERQRDERQKLLVLQAETAGIQLLEPSQE
                     QELRQRLEAQEAKEKEHARNVADTKKAMEWLEDIDRLRKEIAELAGESERLQGELEAF
                     VPEREKLNQAQKAASLDGLYAQLTTTRKQQTEEEIDLEAHTGKVAGLQSLVTERAEAL
                     KNAAQQSLLAKQALKEAGPQLQEVRALDQRLAEQRKMAAEEQKACDKAAATLQAHEKA
                     HGSEQIKRENTGKVLQLASSYLETHAQDEWLVGGLAGVEEQVKGLVDKQKEIARKQGE
                     QVKATAVRDKAAKTVAEREKQSVLRRQSWQQATQKRNEGKEAWEQ"
BASE COUNT          916 a          937 c         1082 g          748 t
ORIGIN      
        1 cgaatcaaaa taactttgac cagtggtatc gttacacgga aaacgtcaac aaagtatatc
       61 aactcttttt gaaattcgct ccccatgagg gtgtgtacac cttcatgagc agcgcgtttt
      121 tccctttgga caacaaaggc tgggggaata cgcccgggta tgaccacaat ttcggtttta
      181 caacggagtt gcatacccga ttcaagtacg aagggggcga aatctttcgg tttacgggcg
      241 acgatgatgt gtgggttttc atcaatgggg agctggcggt cgacctgggg ggcctccatc
      301 ctcctgtcag caaaaccatc gagttggatg ccatcgccgc tgacttcgga ctcgtcaaag
      361 gcaaagccta tagcattgag cttttccacg cagaacgtcg cccggaaggc tccaacttcc
      421 gtgtcgatac caacctggac ttcttggatt gcggaagcgt cgttcctgat aggcccaagt
      481 gaaggacaac ttttattgga aaagcgctgt ttaaagcctc gatgagatgg gtcgtcagaa
      541 tcaatacccc ttcctgacgc tcgatctcca gcaggggctg tttaacccga tagcttgatc
      601 tttcgtacca gagcctcgca cattcccgtt cggtgtgcca atacactggc cccctcgcct
      661 accacaaatg ccatggaacc gggatcgccg cacggcggtg gaaaaaatgg agtgaccgtt
      721 tcgcctggac cgggaccctt cgtgctcgcc gaccctaggg gcgcaatcga gccagcttcg
      781 ccgtccttga cggcatccga cctcggtgta ggcgtaagta tttgtattta catctattta
      841 atttgtaaag aagggatgag gaatccctct ttctcgatta ttcttcttgg atacaccatg
      901 gaacaagcgg tttttcccaa ccatcgaacc gcccacgatg cggaaagggg gcatgaatga
      961 agatcctgca tacgtccgac tggcatcttg gtcgcgttct ttatggcaag aaacgctata
     1021 aggaattcga ggattttctg gactggctcc tcaagacggt aatagatgag aaggtcgacg
     1081 tgcttttggt ggcgggagac atctttgaca cgagcactcc cagcaaccgt gctcaggagc
     1141 tttattaccg ctttttgcat cagctcatcg attcgccgtg ccgccatgcc atattcatcg
     1201 cgggaaacca tgattccgct tcctttctcg atgcgccaaa gcaattgctc gaggcgttgc
     1261 atatccatgt ggtgggaaaa aaacgagacc ctgtcgacga cgaggtgctg gtgcttcgtg
     1321 accgacaagg cgcccctgaa ctcatcgtgg cggcggttcc ttatctgcga gacagcgata
     1381 tccgcgacgc ggaggcaggg gaaagcgtgg aagacaaggg gaaaaagctg atagagggca
     1441 ttcacaggca ctatgccgaa gtggccaagc tggcggaaaa aacacgtcag cagtggggag
     1501 aggatattcc catcgttggg atggggcatc tttttaccag cggcggacgt atcgtggacg
     1561 gcgatggggt gcgcgagctt tatgttggat cgttggctca tgtggacgcg agccgttttc
     1621 cttcgtgttt cgactaccta gccctcggcc acctgcatgt tgcccagaag gtacatggca
     1681 tggaaacggc tcgctatagc ggctcgcctt tgcccatggg ttttggggag gctcatcaga
     1741 aaaaaaaggt ctaccttgtc gaattcgaga aacgaaaggc atcggtggaa tccctcgatg
     1801 tgccggtatc tcaagaactc gagcgaattc aaggaaattg ggacgtcatc tcggcgcgaa
     1861 tccgcacctt ggccgccacc gcatcgaaga aatggcttga agtcatctac gatggcgacg
     1921 aggtgattgg gaatttgcgt gaacggctgg aggctttggt ggaggactcg cccatggaag
     1981 tgcttcgcat taagaataac cgcattatcg aacaagtgat gaaacgctgc cacgacgacg
     2041 aatccctcgg tgaactgaaa gccgatgagg ttttccggcg gtgtctcgag gcgcatgatg
     2101 tgcctgagac ccagcgcccc ggcttgatcc aggcctatca agaagtgctt caatcactac
     2161 tggaagagga tgtccgcgtg caatagggag gcgaacgatg cgaatccttc aagtccgttt
     2221 caaaaacttg aactctttgg agggggaatg gcaaatcgac ctgacccatc cgtcgtttac
     2281 ctccgaaagc atttttgcca ttacaggacc gactggggcg ggcaaaagta cgattctcga
     2341 tgcgatttgc cttgccctgt atgggcgaac ccctcggttg gaaaaggtga cgaaaagcag
     2401 caatgagatc atgtcgcgcc aggccgaaga ctgttttgcc gaggtggtct tcgaaacaga
     2461 taacgggcgt tttcgctgtc attggagcca gcgccgttcc cgcggcaagg cgaaaaacga
     2521 ttggcagccc gcaaaacatg aactcgccaa cgccgattcc ggtgtcattc tcgaagctca
     2581 gatcaggaaa gtggccgctg ccatcgaagc cgcgacgggc atggactatt atcggttcac
     2641 ccgctccatg ctcctggccc aggggcaatt cgcggccttt ctccaggcat ccccggacga
     2701 ccgggcgccg attctcgagc agatcacagg aacggaaatc tatagtgata tttcgatacg
     2761 cgtccatgaa cggcagcgag acgaacggca gaaacttttg gtactccaag cggaaaccgc
     2821 gggaattcag cttctcgagc cgtcgcagga gcaagaattg cgtcagcgcc tcgaggcaca
     2881 agaagcgaaa gaaaaagagc acgcacgaaa cgttgcggat acgaaaaagg ccatggaatg
     2941 gctcgaggat atcgatcggc tacgcaagga gattgctgaa ttggccgggg agtcggagag
     3001 gctacagggg gagttggaag cttttgttcc ggaacgggag aagctcaatc aggcccaaaa
     3061 agccgcttcc ctcgatggtt tgtacgcaca gctcacgacg actcgcaagc agcagacgga
     3121 ggaggaaata gacctggagg ctcataccgg gaaggtagct gggctacagt cgctggtgac
     3181 ggagcgagcg gaagcgctga aaaatgcagc gcaacagagc cttctggcca aacaagcttt
     3241 gaaggaggcg ggacctcagt tgcaggaagt ccgtgcgctt gaccagcggc tcgccgagca
     3301 gcggaagatg gccgcggaag aacagaaagc ttgcgataag gctgcagcaa ccctgcaagc
     3361 gcacgaaaaa gcgcatggat ccgagcagat aaagagagaa aacacaggga aggttctgca
     3421 actcgcctcc tcctatctcg aaacccacgc tcaggatgaa tggctggtgg gcggacttgc
     3481 gggggtggaa gagcaggtca agggattggt ggacaagcaa aaagaaatcg cgcgcaagca
     3541 aggcgagcag gtcaaggcca ccgctgttcg cgacaaggcg gccaagaccg tggcggagcg
     3601 ggagaagcaa tcggtcctgc gcaggcaatc atggcagcag gccacgcaaa agcgcaacga
     3661 gggcaaagag gcgtgggagc agt
//
LOCUS       DAOSOR010000579         3673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_25248_length_3673_cov_3.680763, whole genome shotgun sequence.
ACCESSION   DAOSOR010000579 DAOSOR010000000
VERSION     DAOSOR010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25248_length_3673_cov_3.680763"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(284..562)
                     /locus_tag="PK710_21920"
     CDS             complement(284..562)
                     /locus_tag="PK710_21920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12452.1"
                     /translation="MTAERATHWLQARETSSSRRRHDLQVLRDLVVRWWRDGSDDCAN
                     TESSLVPVTRGGVFSQESHALRAARRAYPFAIGTPRLYETGFRCARDP"
     gene            694..1926
                     /locus_tag="PK710_21925"
     CDS             694..1926
                     /locus_tag="PK710_21925"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HOT12453.1"
                     /translation="MPYRRLQVLLFALSLAPAASCRLGESLTDFSSGSDEGQAAAAGS
                     GNATGGNGEEAGPGDSGGHQGGEGGVAGSGSMGGAGGSGGTGATGGSGGTGGAGGAGG
                     SGGTGATGGSGGTGGAGGAGGSGGTGGTGGSGGTGGAGGAGGSGGTGGAGGTGGSGGT
                     GATGGAGGSSGTGGSPVWQPTPGTSWQYQLSGTIDTTVDAVMYDIDLFDTPGSTIETL
                     RKLGRIVICNFSAGTWEKWREDASSFPPSAIGKPVDGGADEKWLDIRADQVREIMKKR
                     LEQAVQKGCDGVEPDNVDGYRHSTGFSIHSSEQLEFNRFLALEAHGLGLSIGLKNDLE
                     QVSALVADFDWALNEECILWDECQVLAPFISANKAVFHVEYGDDALATKVCPLTAPLK
                     FSTLIKKPELGAWRRACP"
     gene            complement(2251..3552)
                     /locus_tag="PK710_21930"
     CDS             complement(2251..3552)
                     /locus_tag="PK710_21930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12454.1"
                     /translation="MLSEKGQLLRTLAEHGNRKVAERLWHEWFKKASDTEVSILQKAQ
                     KELDLARPPHRGGVFLPLADKKGISGGLLVRVEFSDSPLGQEALDLTSQNAIAEALDA
                     AWKSVRAKGPRPDVYFQFPFASIASVRGTSLWLPGFLAAVAKWGDAVVDTNILATGSM
                     DDDIDLLQAKMRLLEDRGAEIGVDTLWVATRRAPMTVPPKAQVLGDTDEALDRIFSFR
                     PWHHSADVVQCHVHCATRRFDPPARFKEPVTLGFKAYLEPDDLVEVREKVFDALRGPA
                     AELSIAGPVALGAWLGSALRNHKTTVRVVHNDQVWCDNRKRHRISPRDGKPRALLVRC
                     ADDDGENEHHYPIRGVGEVHWTTIRAPGVLTPVDLPNVVEQVILVIGQGEGPVYVAVQ
                     GPIPLAFAMGAALQPLGEHFSFCQLQKTEYIQWFTGQQARI"
BASE COUNT          842 a          995 c         1086 g          750 t
ORIGIN      
        1 aattatcgcg ctggactgcc actcaacgaa ttgaaggggc gcgaaaccta cgttcgagcc
       61 aggaccatcc ggtcatcatc gagcgtcgct cgtgaaaacc agctatcgtt tcccagggct
      121 gaacatcatg agaagcagtg ggcaccaccg agagcggcag gtgcgagttg tcatcgggtt
      181 cgatcatgat gcgcggagcg ttccgaagaa caaataaatt acaataaatt caatatgtta
      241 cactaatatc gcctcccgaa cctttggacc gtagcgcggt gtgttacggg tctctggcgc
      301 agcggaagcc ggtttcgtac agacgaggtg ttccgatagc aaaaggataa gcgcgtcgag
      361 cggcccgcaa cgcatgggac tcctgggaga agacgccacc acgcgtgaca ggcaccaggc
      421 ttgattccgt attggcacaa tcgtcgctgc catcccgcca ccaccgtacc acgaggtctc
      481 gtaggacttg aaggtcatgg cgcctacggg aagaactcgt ttcgcgtgct tgcagccaat
      541 gagttgctct ttctgccgtc atcgcggctt ctagaaaaaa tttttcttgt catattgaac
      601 cgttgcatga aatccgatag aatattcgaa catcgaggcg tcaattcagt ttgttgatgc
      661 ttgtccgtat tgtgtcagac gaaggggaag acgatgccat accgtcgctt gcaggttctg
      721 ttatttgccc tgagtcttgc acctgccgca tcatgtcggc ttggtgaatc gttgacagat
      781 ttttcttcgg gttcggacga aggacaagcg gccgcggcag gcagtggcaa tgccacggga
      841 gggaatggcg aagaagcggg gccaggggac tcgggaggac atcagggcgg agaaggcggg
      901 gtggcgggct ctgggagcat gggtggtgct ggaggctccg gtggaacggg tgcgacggga
      961 ggttctggag gcacgggcgg cgcgggcggt gctggaggtt ccggcggcac gggtgcgacg
     1021 ggaggttctg gaggcacggg cggcgcgggc ggtgctggag gctccggcgg cacgggtggg
     1081 acgggaggtt ctggaggcac gggcggcgcg ggcggtgctg gaggttccgg tggaacgggc
     1141 ggcgcgggtg gtactggagg ttccggcgga acgggtgcga cgggcggtgc tggagggtcc
     1201 agtggaacgg gcggctctcc cgtgtggcag cccaccccag gaacgtcttg gcaataccag
     1261 ctttcaggaa ccatcgacac aaccgtcgat gcagtgatgt acgatatcga tctattcgat
     1321 acgcccggaa gcaccattga aaccctgcgg aagctggggc gcattgtgat ttgtaatttt
     1381 tccgcgggta cctgggagaa atggagggag gatgcgagca gttttccgcc ttctgccatt
     1441 ggaaagccgg ttgacggcgg agcggatgag aaatggcttg atattcgagc cgaccaagtc
     1501 cgggagatca tgaaaaaaag gctggagcaa gctgtccaaa aaggatgcga tggcgtcgaa
     1561 cccgacaatg tcgatggtta tcgccacagc acggggtttt ccattcattc ttccgaacag
     1621 ctcgaattca atcggtttct tgcgctcgag gcacacgggt tgggtttgtc gatcgggttg
     1681 aagaacgatc tcgagcaggt ttcggcgctt gtggctgact tcgactgggc gctcaacgaa
     1741 gaatgtattc tctgggatga gtgccaggta ttggctcctt tcatcagcgc caacaaagcc
     1801 gtgtttcatg tggaatatgg cgatgacgct cttgccacga aagtatgtcc cctgacagca
     1861 ccgctcaagt tttctacctt gatcaagaag ccggagttgg gtgcctggcg gcgggcctgc
     1921 ccctaatagc atgtttcgtg ggctcggacg gctgataggc agtattaccc aaccgccaaa
     1981 gcgccaaaag agaaaagggg ttaaggcgat gcttcccctg caagatatgt gggttttctg
     2041 catgaagcag ggaattcttg aacgcatccc agcttcgttc caagcatccg acaacgatcc
     2101 atcatccctt cgatacgcgc ctgcatggcc cgtattgatt aataaaatct tcccaaacca
     2161 aggaaataat caataaaaac aaggagatag gccagaagcg gcgtacgcga tcgggctgat
     2221 cctcaccctg ctcgatgcgc gaagcattcc tcagatcctt gcctgctgac ccgtaaacca
     2281 ctgaatatat tctgttttct gcaactgaca gaacgaaaaa tgttcgccca gaggttgaag
     2341 agccgcaccc atggcaaacg caagcggtat cggcccctga acagccacgt acacagggcc
     2401 ttccccctgc cctatcacga ggatcacctg ctcgaccacg ttcggaagat cgacaggcgt
     2461 gagaacccca ggggcccgaa tcgtcgtcca atgtacctcc cccacgcccc gaatcggata
     2521 gtgatgttcg ttttcgccat cgtcgtcagc acagcgcacg agcagggcgc gcggcttgcc
     2581 atcccgagga gaaatacgat ggcgtttgcg gttgtcacac cagacctggt cgttatgaac
     2641 gacccgaacg gtggttttgt gatttcgcaa cgcgctaccc agccaggcac caagagcaac
     2701 aggccccgcg atgctcaatt ccgccgccgg tccccgaagc gcgtcaaaaa ccttttctct
     2761 cacctccaca aggtcgtcag gctccaaata tgccttgaat cccagggtaa caggctcttt
     2821 gaatcgcgca ggagggtcaa acctacgtgt ggcacaatgg acatggcatt gaacgacatc
     2881 ggcgctgtga tgccaggggc gaaaagagaa aatacggtcc aaggcctcgt ccgtatcccc
     2941 gagcacttgc gcctttggag gcacggtcat gggagcccga cgagtcgcca cccatagggt
     3001 atccacgcca atctccgcgc cgcgatcttc caaaagtctc atcttggctt ggagcaagtc
     3061 gatatcgtcg tccatactgc ccgtggcaag gatattggta tccaccacgg catctcccca
     3121 cttggcgaca gccgccaaaa atccgggcaa ccagagggat gttccacgaa cggaagcgat
     3181 ggaggcgaaa gggaattgaa aataaacgtc gggtctggga cctttggctc tcacggactt
     3241 ccacgccgcg tcgagcgcct cggcgatggc attttgtgat gttaaatcga gagcttcctg
     3301 cccgagggga gagtcggaaa actcaaccct caccaaaaga ccgcccgaaa tccctttctt
     3361 gtcggcaagg ggaaggaaaa cccctcctcg atgcggagga cgagccaaat ccaactcctt
     3421 ttgtgctttc tgcaaaatag aaacctcggt atcggatgcc ttcttgaacc actcatgcca
     3481 cagacgttcc gccacctttc gatttccgtg ttccgccaaa gtgcgaagca actgcccttt
     3541 ttcactcagc atcgctcaat ctctcaccac aatgcgaccg tccaaaagaa gatacgccac
     3601 cggactgtca aagatgctcg acggcacgat ggcctgatgg gaattggagc gaagggcctc
     3661 gaaaggcacg ctc
//
LOCUS       DAOSOR010000580         3669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_25281_length_3669_cov_4.253182, whole genome shotgun sequence.
ACCESSION   DAOSOR010000580 DAOSOR010000000
VERSION     DAOSOR010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25281_length_3669_cov_4.253182"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PK710_21935"
     CDS             <1..368
                     /locus_tag="PK710_21935"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT12455.1"
                     /translation="FQGNALACSTAVATIDSVRSQKLDERAGRVGNAFQQWLRLAIGS
                     YPQVQAITGAGLMVGVAFDSGTTASTVAHALLQRGYIVVTGGPNANTLTLTPPLTIHE
                     DLLEKFVDCLADVLANLPR"
     gene            376..1515
                     /locus_tag="PK710_21940"
     CDS             376..1515
                     /locus_tag="PK710_21940"
                     /inference="COORDINATES: protein motif:HMM:NF016338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-protein synthetase"
                     /protein_id="HOT12456.1"
                     /translation="MTSPGSRAHCPFDLPASQALHERVRRFIDHQMAGRQSESFDSLA
                     VDIARFQFQCETGVEKLYKAKNIDTGSLTLASQIPAVPTDVFKLRRVACHPAELDTYR
                     FLTSGTTLGARGMHPVRDADTYRRGTRAWASQMLYPDTDRLDWILLAPPFADSPHSSL
                     GFMLDDLCAATQGQATWAVLDGRVDVDAISRALYCANQRQMPIIMAGASFAFVHLLEA
                     LQGRNLALGQRGRVMQTGGFKGKSKEVEALALREQIARSLKIPTDAVISEYGMTELGS
                     QAYEGRWRGWLGLEQPHEDFVCPPWMRVTTVHQETLEPLPDGEIGIARIEDLTNVDSA
                     VAVQTADRVRVMGNRFQLLGRTPGATPRGCSIGIDEILSPESLRP"
     gene            1512..2648
                     /locus_tag="PK710_21945"
     CDS             1512..2648
                     /locus_tag="PK710_21945"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HOT12457.1"
                     /translation="MNALQRERRLMALVQACQTLANPKTPQGKALRKDLLAQGPLSES
                     GVELALTEHLELEPSIQDRKALMAAAGQADVVHVLLSANVFVGALRALALAAVAAPHV
                     VVRMSRRESVFARHLLDAVDKRAFGYPIEIVDSLMIPNSCEGEAHEVHLYGSNSTIVQ
                     VASSLPEFVRIRAHGSGLGLAVVETSCSTNDAAQAVAASMIPFDQRGCLSPRIALVHG
                     DESRAWRFSQALLSCLERAGTKIPLGDLLPEERAERARYQDICRMAGTLFDSPHGCVG
                     LTGGSLWIPPIGRNMHVLACQPAEVETVLRPWLRRIVAAVGIAGGDGGDLATMVQRVV
                     PKARVSAADRMQKPPLDGPVDRRTPEPLAPSEVIRIFRDEYEGG"
     gene            complement(2659..>3669)
                     /locus_tag="PK710_21950"
     CDS             complement(2659..>3669)
                     /locus_tag="PK710_21950"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOT12458.1"
                     /translation="VVIATVHGGGGCKAFEDPDDLSSCQDSEIFEVARDLEPNLIDVL
                     VAGHTHQAIAHRVNGTAIVQSYAYAKAFGRVDLHVNPSTGDVVVLKIHPPHPLCQNES
                     KDYSAACEPGTYEGEPVLPSAVVQQAIAEDMARSEARRLEPVGVTIEADLMDRGRPAS
                     PLSSQLAAWMLEAHPGADVSFVNSGGIRASIPAGPLHYGAFYEMFPFDNRFATATIKA
                     LSFRKLLQDGLVHGHGFAIAGIQANVTCKGEELQVELTKKGRVLRDNDDIVLLTSDYL
                     AMSERFAQAGMEKEAFHVDMGVPIREAIVAYLRKKGGTVAASPSIPVKTPGSWPVTCL
                     VR"
BASE COUNT          788 a         1129 c          995 g          757 t
ORIGIN      
        1 cgtttcaagg aaatgcgctg gcttgcagca ccgccgtggc caccatcgac tccgttcgtt
       61 cccaaaaact cgatgaacgc gccgggcgtg tcggcaatgc cttccaacaa tggctacgat
      121 tggcgattgg ctcctacccc caagtgcaag ccatcaccgg cgcgggattg atggtcggcg
      181 ttgccttcga ttccggcacc accgcctcca ccgtcgccca cgctctcctg caacgaggat
      241 atatcgtcgt caccggcggc cccaacgcca atactctgac cctgactcct cccttgacga
      301 tccacgaaga cctgctcgaa aaattcgtgg actgcttggc agacgtcctt gccaacctgc
      361 ctcgatgacc tgctcatgac atcccctggc tctcgcgctc actgcccctt cgacttgcct
      421 gcatcacaag ctcttcatga aagagtccga cgcttcatcg accaccagat ggccggaaga
      481 caatccgaat ccttcgattc cctcgcggtg gatattgcgc gattccaatt ccagtgcgaa
      541 acaggcgtcg aaaaactata caaagcaaaa aatatcgata ccggctccct gacgctcgct
      601 agccaaatcc cggctgtccc tacggatgtc ttcaaactcc gacgagtggc ttgccatcca
      661 gccgaactcg atacctatcg atttctcaca agtggcacca cactgggggc gagaggaatg
      721 caccccgtgc gcgatgccga tacgtaccgt cgcgggacca gagcttgggc ttcccaaatg
      781 ctctatccgg atacggatag attggattgg atcctgctcg cgcctccgtt cgcggactct
      841 ccgcattctt ccctggggtt catgctcgac gatctatgcg cggcgaccca aggacaggca
      901 acatgggccg tgctcgatgg ccgggtggac gtggatgcga tttcacgcgc gctttactgc
      961 gccaaccaac gccagatgcc catcatcatg gccggcgctt cctttgcctt cgttcacctt
     1021 ctcgaagcgc tgcaaggacg taacctggcc ttgggacaaa ggggacgagt gatgcaaact
     1081 ggcggattca aagggaaatc taaagaagtg gaggccctgg ccctgcgcga acagatcgcg
     1141 cggtccttga aaatccccac cgacgccgtg atatccgaat atggtatgac cgaactcgga
     1201 agccaggcgt atgaaggaag atggagaggg tggctcggac tcgaacaacc ccatgaagac
     1261 ttcgtgtgcc ccccttggat gcgtgtcacg accgtgcatc aagaaaccct cgaaccgttg
     1321 ccggatggcg aaatcggcat cgcccgcatc gaagacctga ccaacgtcga ttccgccgtc
     1381 gccgtgcaaa ccgccgaccg cgtccgcgtc atgggaaatc gcttccaact cctcggacgt
     1441 acccccggtg ccaccccccg gggatgctcg atcggcatcg acgaaatcct gtctcctgaa
     1501 tccttgcggc catgaatgcc ctgcagcgtg aacgaaggct gatggctctc gtgcaggctt
     1561 gccaaacgct tgcgaacccc aaaacccctc aaggaaaagc cctccgaaaa gacctccttg
     1621 cacaagggcc cctatccgaa tcgggtgtgg aactggccct gaccgaacat ctcgaactcg
     1681 aaccgtccat ccaagaccga aaagctctca tggcggccgc tggacaagcg gacgtcgtcc
     1741 acgtgctgct ttccgccaac gtcttcgtgg gcgctctacg cgctttggcc ttggccgccg
     1801 tcgcggcgcc ccacgtcgtc gtgcgcatgt cccgacgaga atcggtattt gcccgtcacc
     1861 tgctcgatgc cgtcgacaaa cgagccttcg gataccccat cgaaattgta gattcgctta
     1921 tgattccgaa tagttgcgaa ggcgaggccc atgaggttca cctttacgga agcaatagca
     1981 ccatcgtcca ggtcgcctcg agtctgcccg aattcgtccg aattcgcgct cacggttcag
     2041 gcttgggcct agcggtcgtt gagacctcct gttccaccaa cgacgcggcg caagccgttg
     2101 cggcgagtat gatccctttc gatcaacggg gatgcctttc accccggatc gcgttggtgc
     2161 atggcgatga gtcacgcgca tggcggtttt cccaagcgct gctctcttgc ctcgaacgcg
     2221 ccggaacgaa aatccctttg ggagatttat taccggaaga acgagccgaa agggcgcgct
     2281 accaagatat atgccgtatg gcaggaacct tattcgattc cccccatggc tgtgtgggcc
     2341 tgacgggcgg tagcctttgg attccgccga tcggacgcaa tatgcatgtg cttgcttgcc
     2401 agcctgcgga agtggaaacg gtgttgcgtc cgtggttgcg acggattgtg gcggcggtgg
     2461 gcatcgcggg cggagacggg ggtgatcttg ccacgatggt tcagcgcgtg gtgccaaagg
     2521 cgcgggtgag cgcggccgat aggatgcaga aaccaccgct ggatggtccc gtggatcgaa
     2581 gaacgcctga acctctcgct ccttcggagg tcattcgtat ttttagagat gaatacgaag
     2641 ggggatgagc gaaagatact accgtacaag gcaggttacc ggccacgagc caggtgtttt
     2701 cacgggtatc gagggggatg cggccacggt gccccccttt ttgcgaagat atgcgacgat
     2761 cgcctcgcgg atgggtactc ccatatcgac gtgaaatgct tctttttcca tgcctgcctg
     2821 cgcgaaacgt tcactcatcg ccaaataatc gctcgtgaga agaacgatat catcgttatc
     2881 ccggagcacc ctgccttttt tggtcaactc gacttgtaat tcttcccctt tgcacgtgac
     2941 attcgcttgg attcccgcaa tcgcaaagcc gtgtccgtga acgagaccgt cttgcagcag
     3001 tttacgaaac gaaagggctt ttatcgtcgc ggtcgcaaaa cgattgtcaa aaggaaacat
     3061 ctcgtaaaac gcgccgtaat gcagtgggcc tgcgggaatc gatgcacgaa tgcctccgga
     3121 attcacgaac gaaacatccg ctcccggatg cgcttccagc atccaagctg ccaattgcga
     3181 cgataacggc gaggcgggac ggcctctatc catcaaatcg gcctcgatcg taacgcccac
     3241 cggttcgagc cgcctggctt cggaacgagc catatcttca gcaatggcct gctgaaccac
     3301 cgcgcttggc agtacaggct ctccttcgta ggttcccggc tcacaagcgg cggaataatc
     3361 cttactttcg ttttggcata acggatgcgg aggatgaatc ttcagaacga ccacgtcccc
     3421 ggtggatgga ttgacatgca aatccacgcg tccaaacgct ttcgcatagg cgtacgattg
     3481 gactatcgcc gtgccgttga cccgatgagc aatcgcttga tgggtatgac ccgccacgag
     3541 aacatcgatg aggttgggct ccaggtcccg ggcaacctcg aaaatttcac tatcttggca
     3601 agaagacagg tcatcgggat cttcgaaagc cttacaaccg ccgcccccat gcaccgtcgc
     3661 gatgaccac
//
LOCUS       DAOSOR010000581         3661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_25347_length_3661_cov_4.705491, whole genome shotgun sequence.
ACCESSION   DAOSOR010000581 DAOSOR010000000
VERSION     DAOSOR010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25347_length_3661_cov_4.705491"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PK710_21955"
     CDS             <1..337
                     /locus_tag="PK710_21955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12459.1"
                     /translation="FDASLPSCLREGLEAPRELSKQAINDGVTRYLHGPQTFQGAVYV
                     ITRAVEQASSGLSVLPEKQRKLIESRVLCRKGWADAARDAGYPTVSAAMRALRPALRA
                     LLKNSLGER"
     gene            449..1165
                     /locus_tag="PK710_21960"
     CDS             449..1165
                     /locus_tag="PK710_21960"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HOT12460.1"
                     /translation="MSVVAHASSPENEVRIVAFDVDGTLVEHDRGLVIWQLLNRRYGG
                     SADINVQRYQAFKSGAITYAQWVDLDIAGWQRQGATRDRIIEVIRKELRLVADARQVT
                     TELKRRGYMVVVVSGTLDIVLETLFPEHPFRHVFTNRIRFDDSGAIEGWTATAYDMAG
                     KAEALRLVSRQTQVPTSAIAFVGDHTNDLEALALAGCPIAYDPKDDALKDVVRHVLPR
                     GQLRRLLDLFPGPEHPLILS"
     gene            1162..2187
                     /locus_tag="PK710_21965"
     CDS             1162..2187
                     /locus_tag="PK710_21965"
                     /inference="COORDINATES: protein motif:HMM:NF012913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOT12461.1"
                     /translation="MSKLPHVLMIHTGGTLMMRPGDDDRILQPDVYARDLLAELPVLR
                     SIASIDTRILFNLDSGDMQPSHWVEIAQCIHESLDEYDGIVVITGTDTMAFTASALAF
                     LLPGLDRPVVLTGAQRPLFDFRTDARSNLIDAFHIATLPVAEVGIAFGHKFLRGCRST
                     KTDAWGLDAFDSPLCQPLVTLGLGVKIGSHVLAARAAGAFDDRIDPRVLCLRVFPGLD
                     PSLIDDALKAGVHGFVLEAFGVGNVPVAHGSLIPSLARASEQDVPVVIVSQCPRGGVD
                     LGRYAGSAAALQAGAISGGDMTVEAAITKLMILLGRAPDTRRRAFILNGFRRALLGEM
                     SVDFPYT"
     gene            complement(2219..2914)
                     /locus_tag="PK710_21970"
     CDS             complement(2219..2914)
                     /locus_tag="PK710_21970"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOT12462.1"
                     /translation="MLAPRVIVFDLDGTLLDTSPDLALSCNHALEVYGRTPLPVEEIQ
                     RYIGDGARRLMSRAARMKDDDPNLDGLVEEFIRHYQQYPVQRTTWMPYATHVLEELSE
                     MLLAICTNKPRSITDAILDTLHASERFALVVGGGDLERNKPSPDPLRFIAKQLRVDPK
                     ELVMVGDGPQDVLAGKSVGARTIAVAAGYTSETFLRELHPDMLLDDLRTLPDIIQRWR
                     EATVRVGRSHASY"
     gene            complement(3011..>3661)
                     /locus_tag="PK710_21975"
     CDS             complement(3011..>3661)
                     /locus_tag="PK710_21975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT12463.1"
                     /translation="VTHRHRDCNLPCVTTAPSRMETSMVTLPDSSVITNAQSGDTKAL
                     EELAKRIRPHVERQLTRYPVSEEDRQDLLQSTLMQVIRKISSFRGDSSFSTWLFRVTA
                     NEALMMMRSHRRHRARLVEGLDMEDLGALVEAQAANHGTHVATSERDARVREALEDLP
                     ESYRTVVVAHYHQDLGLQEIADRFRISESAVRSRLHRARSRLREVLAADSSANIAA"
BASE COUNT          707 a          915 c         1204 g          835 t
ORIGIN      
        1 tttcgatgca agcctgccgt cgtgtcttcg cgaggggttg gaggcgccgc gggagctttc
       61 gaaacaggct atcaatgatg gcgtaacgcg ttatctacac gggccccaga cctttcaagg
      121 agccgtctat gtcatcaccc gcgcggtgga gcaggcttcg tcggggctgt cggtgttgcc
      181 ggaaaagcag cgaaagctca ttgaatcccg tgtgttatgt cgaaaaggct gggcggatgc
      241 cgcaagggat gcgggttatc ctaccgtaag cgcggcgatg cgtgcgctgc gtccagcgct
      301 tcgtgcgctc ttgaagaatt cgttggggga gcgatgacgc aggggcacgg ctgcggacag
      361 tatgcgtgag ccgtggctca taggtgggag aagggtcgac aacccgaccc gataccaact
      421 tacggcaaga tgtactaagc caaagcccat gtcggtcgtt gctcatgcct cgagtccgga
      481 aaacgaggtt cgcatcgtgg ctttcgatgt cgatgggacg ttggtggagc atgatcgagg
      541 cttggtcatc tggcagctac tcaatcggag gtacggtggt tcggcggaca taaatgttca
      601 acgatatcaa gcatttaagt cgggagcgat cacctatgcc caatgggttg acctggatat
      661 cgcgggatgg caacggcaag gcgccacccg ggatcgaatc atcgaggtga ttcgcaaaga
      721 actgcgtctt gtggccgatg cgcggcaggt caccacggag ctgaagcgac gcggctacat
      781 ggtggtggtg gtatcgggca ccctggacat cgttctcgaa acgttatttc ccgagcatcc
      841 atttcggcat gtgtttacga accgcattcg cttcgacgat tcgggggcca tcgagggctg
      901 gacggccacg gcgtatgaca tggcgggcaa ggcggaagcg ttgcgtttgg tttctcgaca
      961 aacccaggtc ccaacgtcgg caatcgcgtt tgtgggtgat cataccaatg acctcgaagc
     1021 gttggcgctc gcgggttgtc ccatcgccta tgacccgaaa gatgatgctt tgaaagacgt
     1081 tgtgcgacac gtcttgcccc gcggacaatt gcgtcgttta ctcgatctat tccccggacc
     1141 tgagcatccg ttgatcctat catgagcaaa ctaccccacg ttttgatgat ccacaccggt
     1201 ggaacgctga tgatgcgtcc cggtgacgac gatcggattt tgcagcctga cgtttatgcg
     1261 cgggatttgc ttgcggagct tccggttctg cgatcgatag catcgatcga cacgcgcatc
     1321 ttgttcaacc tcgattctgg ggacatgcag ccgagccatt gggtggagat tgcgcaatgc
     1381 atccacgagt ccttggatga atacgatggc atcgtggtga tcacgggcac cgatacgatg
     1441 gcgtttacgg cgagtgctct tgcgtttttg ctgccgggcc tcgatcgtcc ggtggtgttg
     1501 acgggcgcgc agcgtccgct tttcgatttt cgcaccgatg cgagatcgaa tttgatcgat
     1561 gcgtttcaca ttgcgacgtt accggtggcg gaggtgggca tcgcgtttgg gcacaagttt
     1621 ttgcgggggt gtcgaagcac gaaaaccgat gcgtggggat tggatgcttt cgatagtccg
     1681 ttgtgccagc cgctcgtgac gttgggattg ggtgtgaaga ttggatcgca tgtgttggcg
     1741 gctcgtgccg ctggcgcgtt cgatgatcgg atcgatccgc gtgtgttatg ccttcgcgtg
     1801 tttccggggt tggatccctc gctgattgac gatgcgttga aagcgggggt tcatgggttc
     1861 gtgctcgagg cgtttggtgt gggcaatgtt ccggtggccc acggttcgct gattccttcc
     1921 cttgcacgcg cttccgaaca agatgtaccc gtcgtcatcg tcagccaatg cccgcgcggc
     1981 ggagtcgatc tcggacgcta cgcgggctcg gccgcggcgc ttcaagccgg agcgatatcc
     2041 ggaggagata tgacggtcga ggccgcgatt accaagctga tgattctgtt ggggcgagcc
     2101 ccggatacgc gtcgtcgcgc cttcattctc aatggttttc gtcgtgcttt gctcggggaa
     2161 atgagcgtgg actttcccta tacgtgagcg acggcaggcc atgagtggcc actagggtct
     2221 agtaggaagc atgggaccgg cccacgcgaa cggttgcctc tcgccaacgt tggatgatgt
     2281 cgggaagggt tcgcaggtcg tcgagcaaca tatcggggtg caattctcga aggaaggttt
     2341 cggacgtata gccagcggcg acggcgatgg ttcttgcgcc aacacttttt cctgccaaaa
     2401 catcttgcgg gccatcgccg accatcacca attccttggg gtcgacgcgc aattgtttgg
     2461 cgatgaatcg taagggatcg ggagaaggtt tgtttctttc cagatctccg ccgccgacga
     2521 cgagggcgaa gcgttcgctg gcgtgaaggg tatccagaat ggcgtcggtg atcgaccgag
     2581 gcttattcgt gcaaatggca agcagcatct cgctcaattc ttcgagaaca tgcgtggcgt
     2641 agggcatcca cgtggttcgt tggacgggat attgctggta gtgacggatg aattcttcga
     2701 ccaggccatc gaggttgggg tcatcgtctt tcattcgtgc cgcacgcgac atgagtcgtc
     2761 gtgcgccatc gccgatgtag cgttggattt cctctacggg cagaggggta cgaccgtaca
     2821 cctcgagggc atgattgcag gacaatgcga ggtcgggact ggtatcgagg agtgtgccgt
     2881 ccaggtcgaa aacaatgacg cggggtgcga gcatgtgtga gacatactcg cacgaacggc
     2941 tgaattatcc aagggcaact tggctgcgga ggcggagggg tgcaggtggg ataggggaag
     3001 gaaggcgtga tcaggcggcg atgtttgcgg acgagtctgc ggcgagcacc tcgcgcaggc
     3061 gagaacgtgc gcgatgcaag cgtgaacgaa cggctgattc ggatatcctg aatcggtctg
     3121 cgatttcctg aagaccgagg tcttgatggt aatgagcgac caccacggtc cgatacgatt
     3181 caggcaagtc ttcgagcgct tctctcacgc gggcgtctcg ctcgcttgtg gcgacgtggg
     3241 tgccgtggtt ggcggcctgg gcttcgacga gtgcgccgag gtcttccata tcgagacctt
     3301 cgacaaggcg tgcccgatgc cgacgatggg agcgcatcat catgagggct tcgttggcgg
     3361 tgacgcgaaa cagccaggtg gaaaaactcg aatcaccacg aaaagaagat attttgcgaa
     3421 tgacctgcat caacgtgctt tgcaacaagt cttgtcggtc ttcttcggaa acgggataac
     3481 gggtgagttg tcgttccacg tgggggcgaa tgcgtttggc aagctcttcc agggccttgg
     3541 tatccccgga ttgggcgttg gtaatgactg aagaatcagg aagggtaacc atgcttgtct
     3601 ccatgcggct tggggccgtc gtgacgcagg ggagattgca atctcggtgc cgatgagtaa
     3661 c
//
LOCUS       DAOSOR010000582         3660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_25356_length_3660_cov_4.056865, whole genome shotgun sequence.
ACCESSION   DAOSOR010000582 DAOSOR010000000
VERSION     DAOSOR010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25356_length_3660_cov_4.056865"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1739)
                     /locus_tag="PK710_21980"
     CDS             complement(<1..1739)
                     /locus_tag="PK710_21980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HOT12464.1"
                     /translation="MNEIANDTSATSAVAPTTYDSDKITVLEGLEAVRKRPGMYIGDV
                     HDGSGLHHLVWEAVDNAVDEHLAGACTHIDVTVHFDGSVTVEDNGRGIPVGMHEKGVS
                     AAEVVMTVLHAGGKFDHSSYGVSAGLHGVGVSAVNAVSEWLKMEIRREGKVWFQEYRK
                     GIPVDRLLPIGETTHSGTKISFKPDTEIFTNTEYSFDVLAKRLREMSFLNAGLVITLT
                     DEREGDRRKVYEYKGGIREFVDLLNRNKEPIHEEVIYIEASAQHENNLPIQVAIAIQW
                     NASYQEQVFPYTNNVHNKDGGTHLTGLRAGLTRTLNQYALQLNLVKEKNALSGEDCRE
                     GLTAVISVKHPDPSFDSQTKSKLVSSEVKGLLEGIIVDKLGQVFEENPATARRIIEKA
                     VVAAKARDAARKARENVRKGSQDLTSLSGKLADCQSKDPTKCELYIVEGESAGGSAKQ
                     GRDRHFQAILPLRGKILNVERARTDRMLASEMIGILITALGCGIDDAGGLDISKLRYH
                     RIILMTDADVDGSHIRTLLLTFFYRKMPEIIEKGYLYIAQPPLFRVRKGKKDLYLKDE
                     KALESFLVDNAIEG"
     gene            complement(1904..3022)
                     /gene="recF"
                     /locus_tag="PK710_21985"
     CDS             complement(1904..3022)
                     /gene="recF"
                     /locus_tag="PK710_21985"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOT12465.1"
                     /translation="MAARQQASAKLEALEVRNIRNIEALGLDPSPGTNVICGANGQGK
                     TSLLEAIYLVATTRSFRTHRLRETIRHGTDGGSAKARFEDMLGDREQVVGLTPTQRSV
                     RVDGQRPASLAQYAVKTPVVVFHPGELALTMGPASGRRTLLNRIALFFDPTTHAVQHA
                     YGEAMRARQRLLQVQGPQAQGLEAYERLMVEHGMRITRFRQDVAEKLANVARGVLDQA
                     TFGSLRLDARYVPGGSLDEQEECKRLVASRSQDCRRSSSTHGPHRDEFLLEINGASTR
                     TDASQGQHRLLTLCLKIAEMVCVGQARGVNPILLLDDVSSELDAERTEAFFDLLGHRN
                     EQVFLTTTRAEMLSVFERKMGETNVFWMKGGQIGGKPW"
     gene            complement(3022..>3660)
                     /locus_tag="PK710_21990"
     CDS             complement(3022..>3660)
                     /locus_tag="PK710_21990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOT12466.1"
                     /translation="IRMVTTDGHRLTKVEATVPGRHATATMLIPYKAITELRKLTDEF
                     KGLDAKSESPTMVTITQFGPNAFFSIGDTRFSVKLVDAQFPAYQQVIPKSSERVVRAP
                     RAILMDALRAVSLAASDRTGGVQLSIESGTMRILSESPESGDGSDELPVEYTGTAMKI
                     GFNAKYLLDVLAVLDDEEVDLLLSGELDPAIIKPTGESDGRSFVGVVMPMRI"
BASE COUNT          813 a         1132 c          854 g          861 t
ORIGIN      
        1 ccttcaatgg cgttatccac caaaaagctt tcgagcgcct tttcatcctt gagataaagg
       61 tcttttttgc ctttgcgaac acgaaacagc gggggttggg cgatgtacaa gtatcccttt
      121 tcgatgatct ccggcatctt ccgataaaag aaggtgagca acaacgttcg gatatgcgac
      181 ccgtccacat ccgcgtccgt catcaaaatg atgcgatggt agcgaagctt cgagatatcc
      241 aaaccacccg catcgtcgat accacaccca agcgcggtga tgagaatgcc gatcatctcg
      301 gaggcaagca tgcgatcggt acgggcacgc tcgacattga gaatcttgcc tcgaaggggc
      361 aaaatcgctt ggaagtgacg atcccttcct tgtttggcgc ttcctcccgc gctctctccc
      421 tcgacaatgt atagctcgca cttggttggg tccttggact gacaatccgc aagctttccc
      481 gacagagagg tgaggtcttg cgagcctttt cgcacgtttt cacgcgcttt tcgcgcggca
      541 tcgcgagctt ttgccgctac cactgctttc tcgatgatgc gacgcgccgt cgccggattc
      601 tcctcgaaaa cctgtcccag cttatcgacg atgatgcctt ccagaagccc tttgacctcg
      661 ctcgacacaa gcttggactt cgtctgcgaa tcaaaagaag gatcgggatg tttgacgctg
      721 atgaccgccg tcaatccttc acggcaatcc tctccggata aggcattttt ctctttcacg
      781 aggttgagct gcaaagcgta ttggttgagc gttctcgtca atcctgcgcg caatcccgtc
      841 agatgggtcc ccccatcctt gttgtgtacg ttgttcgtgt aaggaaaaac ctgttcttga
      901 tagctcgcgt tccactgaat cgcgatggcc acctggatgg gtaggttatt ttcgtgttgc
      961 gcggaggcct cgatataaat gacctcctcg tgaatgggct ccttgttgcg attgagcagg
     1021 tccacgaatt ccctgattcc acccttgtat tcatatacct ttcgacgatc cccttctcgt
     1081 tcatccgtga gtgtgatgac caaccccgcg ttgagaaaag acatctcacg cagacgcttt
     1141 gccaacacat cgaagctgta ttccgtgttg gtaaagatct ccgtatccgg cttgaaggat
     1201 attttggttc cgctgtgtgt cgtttcccca atgggaagga gccgatcgac cgggattcct
     1261 tttcgatact cttgaaacca gaccttgcct tcccgtcgga tttccatttt caaccattcg
     1321 gaaaccgcgt tgaccgccga aacgccaacc ccatgtagtc cagcggatac accatagctc
     1381 gaatgatcga acttaccgcc agcgtgcagt accgtcatga ccacttcggc cgcgctaacc
     1441 cctttttcat gcataccaac cggaattccc cgtccgttat cctccaccgt caccgaacca
     1501 tcgaagtgaa ccgtgacatc aatatgcgtg catgctccgg cgagatgctc gtctaccgca
     1561 ttgtccaccg cttcccatac caagtgatgc agtcccgaac catcatgcac atcaccgatg
     1621 tacatacctg gtcgtttacg aactgcctcc aatccttcga ggaccgtgat tttgtcggaa
     1681 tcatacgtgg tcggcgcgac cgcggaagtc gcggatgtgt cgttggcgat ttcgttcatg
     1741 ggcgtcttgt ttctacggga cggcgggcct ttggccgcag ggccaaaggg tcatgggcca
     1801 tgggatgaac gtacggcccg gttcgcctag aaagtgagct ttcttagcgc atcgatggca
     1861 cctgagcaag cgctgccccc gattcccatg aatcgtttca cggtcaccat ggcttccccc
     1921 caatctgtcc ccccttcatc caaaaaacgt tcgtttctcc catttttctc tcgaataccg
     1981 acagcatctc cgcacgggtc gtcgtcaaga acacctgctc gttccgatga cccaacaaat
     2041 caaaaaacgc ttccgtccgc tccgcgtcca actcactcga tacatcgtcg agcaacaata
     2101 tcggattgac cccccgagct tgcccaacac ataccatttc cgctattttc aaacacaacg
     2161 tcaatagacg atgttgccct tgcgaggcgt ccgttcgagt ggacgcccca ttgatttcaa
     2221 gaagaaactc atcacggtga ggtccgtggg tcgaggatga ccttctacaa tcttggctac
     2281 gagatgccac cagacgcttg cattcctctt gctcatcgag agaaccaccc gggacatacc
     2341 gcgcatcgag ccgtaatgaa ccgaacgttg cttggtccaa tacacctcgg gccacattgg
     2401 cgagtttttc agccacatcc tgtcgaaagc gtgtgatgcg catcccatgc tccaccatca
     2461 accgctcata cgcttccaaa ccctgcgctt gcggaccttg cacttgcaac aatcgctgcc
     2521 gcgcgcgcat cgcctcgcca taagcatgtt ggacagcatg ggtagtcgga tcgaaaaaaa
     2581 gcgcgatacg attcaataac gttcgacgac ccgacgctgg acccattgtc aatgcaagct
     2641 cgcccggatg aaacacaacc acgggagttt tcacggcgta ttgcgccaaa ctcgccggcc
     2701 gttgcccgtc cacacgcacg gaacgctggg ttggcgtcaa accaaccact tgctcccgat
     2761 ccccaagcat gtcttcaaat cgagctttcg cactaccccc atccgtacca tggcgaatcg
     2821 tctctcgaag ccgatgggtc cgaaaactcc gcgtcgtagc caccagataa atcgcttcga
     2881 gaaggctcgt tttcccttgc ccattggcac cacaaatcac attggtgcca ggcgatgggt
     2941 ccaacccgag agcttcgatg ttccgaatgt ttcgaacttc gagcgcctcg agcttcgctg
     3001 atgcttgctg tctcgccgcc atcaaatccg catcggcatc acaacaccca cgaaacttcg
     3061 accgtcggat tctcccgttg gtttgataat ggccggatca agctctccag ataacaacaa
     3121 atcgacttct tcgtcgtcga gaaccgcaag aacatcgagc aagtacttgg cgttgaaccc
     3181 aattttcatc gccgtgcccg tatactcgac gggtagctca tcggaaccat caccgctttc
     3241 cgggctctca ctcaaaatgc gcatcgtacc cgactcaatc gaaagctgaa ccccacccgt
     3301 acggtcactc gccgccaacg atacggcccg gagagcatcc atcaatatcg cccgtggcgc
     3361 gcgaaccact cgctccgatg acttggggat gacctgctgg taggcaggaa actgcgcatc
     3421 cacgagcttg acggaaaaac gagtatcccc aatgctgaaa aacgcgtttg gaccaaattg
     3481 cgtaatggtc accatcgtgg gcgactcgct cttcgcatcc aaccccttga actcatccgt
     3541 cagcttccgc aattccgtga tggccttgta cggaatcaac atcgtcgccg tcgcatgacg
     3601 acccggtacc gttgcttcca ccttcgtcag tcgatgacca tccgtagtca ccatgcgaat
//
LOCUS       DAOSOR010000583         3644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_25505_length_3644_cov_3.662580, whole genome shotgun sequence.
ACCESSION   DAOSOR010000583 DAOSOR010000000
VERSION     DAOSOR010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25505_length_3644_cov_3.662580"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1851
                     /locus_tag="PK710_21995"
     CDS             <1..1851
                     /locus_tag="PK710_21995"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOT12467.1"
                     /translation="GQVVGKSASSGNSSGPHLHLAWRNSSNVDKNPYVGSCSATSVTA
                     WVDQGTYWGSTSAQCECAASPEVCNGKDDDCDGLVDEDDVCEQEALLLGQSWVAPART
                     TDIDGDGQSDICGRGMAGVWCHLSEGTSWSSKGPTLPLSDASGWSDPTNWGTFRMGDI
                     DGDGRADLCARSDERVQCWKSDGTSLSIAVEGPGWADASGWGNFQYHSTLRLLDIDGD
                     GKDDLCARAAKGIVCLKSTANGFGPSLEGPAWSDAQGLDKAKYYGTLRTGDVNGDGKD
                     DLCMRTQEGVECWLSDGDGFSTRVQGPGWSDGSGWGEMPYWSTIRLADVNGDGRADLC
                     ARAAAGLRCHFSTGNGFGDAVIVAALSDDTGWADPSNYLTLRTGDVDGDRAQDLCIRA
                     NAKVICYRWDGSAFVSFDGPAWSDEEGWNKPQYFHTIQLGDMNGDGKADLCGRHVTGW
                     RCHPSTGTGFGAEVLLDEFKDTGGWSSDKYYGTLQFGGPLCIPHEEICNGIDDDCDGE
                     VDEGGVCLTDGGAGGSGGSDASSQTGGSSGSGNDAGKDSRSESDPSQPPSPSQPSSPD
                     TNYYPVSETSDASGCACTTTSNASNTFYGWMLVGAVTLLRSRRKTQSKRS"
     gene            complement(1918..2154)
                     /locus_tag="PK710_22000"
     CDS             complement(1918..2154)
                     /locus_tag="PK710_22000"
                     /inference="COORDINATES: protein motif:HMM:NF020917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HOT12468.1"
                     /translation="MGEPKIAFKAPAVLELEAGTYWWCKCGLSANQPFCDGSHKTTEF
                     TPMKLELTEKKTVALCQCKHTGNPPFCDGTHNKL"
     gene            complement(2165..>3644)
                     /gene="gpmI"
                     /locus_tag="PK710_22005"
     CDS             complement(2165..>3644)
                     /gene="gpmI"
                     /locus_tag="PK710_22005"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOT12469.1"
                     /translation="HGTAVGLPSDGDMGNSEVGHNSLGAGRVFDQGSKLVGRAIETGS
                     LFQGQTWTSFIQQVISAHTALHFIGLLSDGNVHSHIDQLFALLRRADQEGVQRCFVHP
                     LLDGRDVPETSALQFIEPLEKLLADISSKPDRTYAIASGGGRMLVTMDRYNANWSVVE
                     RGWNAHVHGQGRRFPSATAAIESYRQQHPGLIDQNMPEFVIEKDGKPIGAMNDGDAVV
                     FFNFRGDRGIEISRAFDEDSFHEFDRGQRPRVMYAGMMQYDGDLMIPKKYLVDPPAID
                     RTMGEYFAHNGVKQLAISETQKYGHVTYFWNGNRGGKFDEKLETYVCIPSDTLPFEER
                     PWMKAAEITDRFIEELRSGSYRHLRLNYPNGDMVGHTGVRDAAIQAVEAVDLSIARLM
                     AATRQVEGILIVTADHGNADEMYELDKKGGFKCNADGRPRSKTAHTLNSVPFVIYDPG
                     FAGEYRLAELERPGLSNVAATALSLMGYEPPEGYDPPLIQFS"
BASE COUNT          765 a          953 c         1111 g          815 t
ORIGIN      
        1 ggccaagtcg tgggtaaatc cgcgagttct ggtaattcaa gcggccctca ccttcatttg
       61 gcctggcgca attcgagcaa cgtcgacaag aacccttatg ttggctcctg ttcagcgact
      121 tccgtcaccg cgtgggtcga tcaaggaacg tattggggat ctacaagcgc ccaatgcgaa
      181 tgcgcggcat cgccggaggt gtgcaacggc aaggacgatg actgcgatgg attggtggac
      241 gaagacgatg tgtgtgagca ggaggctttg cttctcggtc aatcctgggt tgctccggcg
      301 cgcaccaccg atatcgatgg ggacggccaa tccgatattt gtgggcgtgg catggcagga
      361 gtttggtgcc atctatcgga aggaacttcg tggagttcca aaggaccaac gcttcccctt
      421 tcggatgcta gcggctggag tgatccgacc aactggggaa cctttcgcat gggcgacatc
      481 gatggcgatg ggcgagcaga cttgtgtgcg cggagcgatg aacgcgtgca gtgttggaag
      541 tccgatggaa ccagcctgag cattgcggtc gaaggccctg gatgggcaga cgcgagcggc
      601 tggggcaact tccaatatca cagcacactg cggttgttgg acatcgatgg tgacggcaaa
      661 gacgatctat gcgcgcgtgc ggccaagggg atcgtatgcc tcaagtccac ggcgaacggc
      721 tttggtcctt ccttggaggg gcctgcctgg tcggatgccc aagggcttga taaagcaaag
      781 tattacggca cgttacgaac gggtgacgtg aacggagacg gaaaagacga cttgtgtatg
      841 cgcacgcaag agggggtgga gtgctggcta tccgacgggg atgggttttc aactcgcgtg
      901 caaggccctg ggtggagcga cggttcgggg tggggagaga tgccgtattg gagcacgatc
      961 cggcttgccg acgtcaacgg ggatggtcgc gcggatctat gcgcgcgtgc ggcagccggc
     1021 ttgcgatgtc atttttccac cggaaacggg tttggagatg cagtgatcgt agccgcgttg
     1081 tcggatgaca cgggctgggc ggatccttcg aactacttga cgttgcgaac gggcgacgtg
     1141 gatggcgatc gagcgcaaga tttatgtatt cgcgccaacg cgaaagtcat ttgttatcgg
     1201 tgggatgggt cggctttcgt ttcgtttgac gggcctgcct ggtcggatga agaaggatgg
     1261 aacaagccgc aatactttca tacgattcaa ctgggcgaca tgaatgggga tgggaaggcg
     1321 gatttatgtg gtcgccacgt gacgggatgg cgctgtcatc cttccacggg tacgggtttc
     1381 ggagcggaag tcctgctcga tgaattcaag gacacgggag gatggtcgag cgacaagtat
     1441 tatggaacgt tgcagtttgg cggtccgttg tgcattcccc acgaggaaat ttgcaacgga
     1501 atcgatgacg attgcgatgg tgaagtggac gaaggagggg tgtgccttac ggatggggga
     1561 gcgggaggaa gtggtggttc cgacgcttct tcgcagaccg gtggatcttc cggaagcggc
     1621 aacgatgctg gaaaggatag ccgttccgag tctgacccct cccaaccccc ttcaccatcc
     1681 cagccctctt caccagatac aaactattat cccgtaagcg agacttcgga tgcgtcgggc
     1741 tgtgcctgca ccacgacatc gaacgcgagc aataccttct atggctggat gctcgtcggg
     1801 gctgtcaccc tgcttcgctc tcgaagaaaa actcaatcga agcgctcgtg atcccgtggt
     1861 ggatctggtc gagtccatcg actcctgtca ctcaccaatc cgcgcgcgcc aagcccttta
     1921 gagtttgtta tgggtgccgt cgcaaaacgg tgggttgccc gtatgcttgc actggcagag
     1981 ggcgacggtt ttcttttcgg tcaattcgag cttcatcggg gtaaattccg tggttttatg
     2041 ggagccatca cagaaaggct gattggcgga caaaccgcat ttacaccacc aataggttcc
     2101 agcttcgagt tcgagtaccg cgggcgcttt gaatgcaatt ttcggttcac ccatttcgat
     2161 cacctcaaga gaactgaatg aggggcggat catatccttc cggcggctca tatcccatca
     2221 aagacagagc ggtggcggca acattggaca gaccggggcg ttccaattcg gccaaccggt
     2281 actcaccggc gaaccctgga tcataaatca cgaaaggaac ggaattgagt gtatgcgccg
     2341 ttttcgaacg cggccgccca tcggcattgc acttgaaccc gcctttcttg tcgagttcgt
     2401 acatctcgtc ggcattgccg tggtccgctg tcacgataag gataccttcg acttgtcggg
     2461 tggccgccat cagtcgtgcg atggaaagat caacggcttc caccgcttga atggccgcat
     2521 cccgcacgcc agtatgtccg accatatccc cattcggata gttgagccgc aggtggcgat
     2581 acgatccgct gcggagttcc tcgatgaatc gatccgtaat ctccgccgct ttcatccacg
     2641 gccgttcttc gaacggcagc gtatcgctcg ggatgcacac atacgtctcc aacttctcat
     2701 cgaattttcc accccggtta ccattccaaa aataggttac gtgtccgtat ttctgcgttt
     2761 cactgatcgc caattgtttg acaccgttat gcgcgaaata ctcccccatg gtgcggtcga
     2821 tggcgggagg atccaccagg tactttttcg gaatcatcaa atcgccatcg tattgcatca
     2881 tcccggcgta catcacccga gggcgttgac cgcgatcgaa ctcgtggaag gagtcttcgt
     2941 cgaaagcacg gctgatctcg attccgcggt ctccccggaa attgaagaaa acgacagcat
     3001 caccatcgtt catggcacca atcggcttgc cgtctttctc gatcacgaac tcaggcatgt
     3061 tttgatcgat gagtccgggg tgttgttgac gataggattc gatggccgca gtggcagagg
     3121 ggaagcgtcg tccttgccca tgaacatggg cattccatcc tctttcgacg acactccaat
     3181 tggcgttata gcggtccatg gtgacgagca ttcgaccgcc gccagaagcg atggcgtagg
     3241 ttcggtccgg cttcgaggag atgtcagcga gaagcttttc gagcggttcg atgaattgca
     3301 acgcgctggt ttcggggaca tcgcggccat cgagcagcgg atgaacgaaa cagcgttgga
     3361 ccccttcctg gtcggccctt cgcaacagtg cgaacaactg atcgatatgg ctatgaacgt
     3421 tgccgtctga caataggccg atgaagtgca aggcggtatg cgccgagatg acttgttgaa
     3481 tgaacgacgt ccaggtttgt ccttgaaaga gcgagcctgt ctcgatggcg cggccgacga
     3541 gtttggagcc ttgatcgaaa acgcgtccgg ctcctaacga attatgaccc acttcgctgt
     3601 tgcccatgtc gccgtccgac ggcaggccaa ccgccgttcc gtgc
//
LOCUS       DAOSOR010000584         3639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_25553_length_3639_cov_4.202009, whole genome shotgun sequence.
ACCESSION   DAOSOR010000584 DAOSOR010000000
VERSION     DAOSOR010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25553_length_3639_cov_4.202009"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PK710_22010"
     CDS             complement(<1..377)
                     /locus_tag="PK710_22010"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT12470.1"
                     /translation="MLLEQLMVIVTGGAQGMGRHFSKRLVQAGARVVAADVNEEGLAS
                     LHRECEAEAGKLWVRKLDVSDEQQCIDSVGWAYNQMGGLNALVNNAGILRDGLLVKLD
                     RKTGQIVKLSRAQWDAVIAVNLTG"
     gene            complement(497..1036)
                     /locus_tag="PK710_22015"
     CDS             complement(497..1036)
                     /locus_tag="PK710_22015"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT12471.1"
                     /translation="MTESTTLREIQDRYPLLDVFPETDGAPQLVLCVMRTAALLERVV
                     ARALMPFRLHYAQFNALVLLDREREGLRPSVLGSYLCVSRPNVTKLLARLKDRGLVVE
                     KSDPQDGRAVLAVITEEGIGLVQLAFVAVTRDLSTVVETLPQQDGAHLRTVLDRFRNG
                     LFQALRASGCEEDSGLASI"
     gene            1421..3316
                     /locus_tag="PK710_22020"
     CDS             1421..3316
                     /locus_tag="PK710_22020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT12472.1"
                     /translation="MSSSVRLSRCIDFNGMLEALPEPAMLIHLASGRITGVNSLACSL
                     LNLPAHQLIGMKAVDSRASGHRATFEAALLALSKTYNRPPAVVNEVLMGAHRCLHAVS
                     SRFGYFEIENEPFALHFFSERLEDGAEPARQSTIQHKNEFFLSAARELRNPLTSILGF
                     LDLISEPESSERDRGVWIAEIRKSSEMLMGIVQNLLDLGQMETGSFQLEHDAVPTSLA
                     LAHVLSQARTRGQHLGHDVIARYTTPVPHTLTLDPTRFRQCLSTLIHFVIENTSRGDV
                     TLQTAIIGHENQRQLAFDVSATCTDLDPQDLESLLDPWQSPTMRARSWKLAFTVAAKI
                     SIMMGGNIQGGMDAGRIGMRLTIQPNPSDFQQMVHLSLPEETQGRPSTEPPLRLRGSV
                     LLIENNDSDIAFLSKILRGSGLRVDHAPSLDLQSHPFQRYDAVLVGIDLPHANGLEPV
                     EKLRTSGVTVPIVALTTSTCPHDHNRCLESGCTAVVTKPIERTTLLQRLDDLISTTED
                     RRVMSVLPPDIAIADPLPSTLETEPSVRQILPQLVMRMPRLIKHIAQAIRRLDLPTAA
                     RTARSLGATAGNCGFESLSKAAHALADACEHPPIDTDVLGDRLEQLENMAKRVQAAYP
                     DFIKPSE"
BASE COUNT          814 a         1193 c          851 g          781 t
ORIGIN      
        1 cccgtcagat tcaccgctat caccgcatcc cattgcgcac gcgacaattt gacgatctga
       61 ccggtctttc gatcgagttt gacaagaaga ccatctcgta aaatccccgc gttgttgacc
      121 agagcattca acccgcccat ctggttgtag gcccaaccca ccgaatcgat acactgctgc
      181 tcgtccgaca catccaactt tcgaacccac agctttccag cctctgcctc acactcgcga
      241 tgcagtgatg caagcccttc ttcattcacg tccgccgcca cgaccctggc ccccgcctgt
      301 accaagcgtt tggagaaatg ccgccccatt ccttgagccc cccctgtcac aatgaccatc
      361 aactgttcga gcagcatgca gcaacctcct cgggcgcgag cgcatatcgg ctagtcgacg
      421 acgcggcaca accccgctta cccgtgcgac ataacacgtc cagcgtcaga ctgtcagaca
      481 aagctttgtc ggcaagtcaa atgctcgcca aaccggagtc ttcctcgcac cccgaagcgc
      541 gcaaagcctg aaacaaccca ttgcggaaac ggtccaacac cgttcgcaaa tgcgcgccgt
      601 cttgttgcgg caacgtctct acaaccgtcg aaagatcccg ggtgaccgcc acaaacgcca
      661 gttgaacgag accaattccc tcttccgtga tgacagcaag tacggcgcga ccatcctgcg
      721 gatccgattt ttccaccaca agcccccggt ccttcaatcg cgccaacaat ttcgtgacgt
      781 tgggacgaga aacgcacaga tacgaaccca acacgctcgg tcgtaacccc tcacgctcgc
      841 ggtcgagcaa cacaagcgcg ttgaactgcg cataatgaag ccgaaacggc atcagcgctc
      901 gtgcaacgac ccgctccaac agcgcagccg tgcgcatgac gcataaaacc aattgtggtg
      961 cgccatccgt ttctggaaac acgtcgagaa gcggataacg atcttggatt tcacgaagtg
     1021 ttgtggattc agtcacggga ccaccatagc cgacatcaag gttgccaggg cgagagcttc
     1081 gattcactcc gaccatcgtt gtttccatgg ggcccccagc cgtcgataac cacgaaattc
     1141 cgctagattt tccggtgcgg accggatgct cgttcgaatc ccataattca tatttatatt
     1201 ttttaggtaa ttacagagat atcacccaga acctgcccgt cccaacctca ccctgcaccg
     1261 cggcaggttc agtgctatga ttgactctgt ctccgatcac ttcgtgagat cagtcgtggt
     1321 gggccaccat gggaacggga agcttgtctc cctgtcgtgg tttttgcttc gcttgcttca
     1381 tggactcttc acggtctgtc tttggggaaa cacgtaggcc atgtcctcat cggttcgatt
     1441 gtcaagatgc atcgacttca acggcatgct cgaagctttg cccgagcccg ccatgctcat
     1501 ccatctggct tcgggtcgaa tcaccggcgt caactccttg gcctgctccc ttttgaacct
     1561 gcccgcccat caactcatcg gcatgaaagc cgtcgactca cgcgcctctg gtcaccgcgc
     1621 caccttcgaa gcagctttgc tcgctctttc gaaaacgtac aatcgacccc ctgctgtcgt
     1681 gaatgaagta ttgatgggcg ctcatcgctg tctgcatgcg gtcagttcgc gctttggata
     1741 cttcgaaatc gaaaacgaac ccttcgccct tcattttttc tccgagcgac tcgaagatgg
     1801 tgcggagccg gctcgacaaa gcacgatcca acacaaaaac gaattcttcc tatcggccgc
     1861 ccgcgaactc cgaaatccgc tgacgtcgat tcttggtttt ctcgacctca tttccgaacc
     1921 ggaaagcagc gaacgggatc gcggcgtatg gatcgcggag atccggaaaa gtagcgaaat
     1981 gctgatgggc atcgtgcaga acttgctgga cctggggcaa atggaaacag gctcgttcca
     2041 actcgaacac gacgccgtcc cgacaagcct tgcccttgcc cacgttctat cccaggccag
     2101 aacgaggggc cagcacctgg gtcacgacgt cattgctcga tatacgaccc ccgttcccca
     2161 caccctcacc ctcgatccca ctcgctttcg acaatgcctt tctaccctca ttcatttcgt
     2221 catcgaaaac acgtctcgcg gtgacgttac gttgcaaacc gccatcatcg gtcacgaaaa
     2281 ccaacgacaa ttggcctttg acgtttcggc cacgtgtacc gacctcgatc cacaagacct
     2341 ggagtcattg ctcgacccgt ggcaatcgcc gacgatgcgg gcgcgttctt ggaaactggc
     2401 attcaccgtt gcggcgaaaa tctcgataat gatgggggga aacattcaag gaggtatgga
     2461 tgccggccgc atcggaatgc gattgaccat tcagcccaac ccatcggact tccaacagat
     2521 ggtccatctg agtctgccag aggaaacaca aggccgaccg tcgacagaac ccccgttgcg
     2581 tcttcgaggc tccgtattgc tcatcgaaaa caacgattcg gatatcgcct tcctttcaaa
     2641 aatccttcgc ggcagcggat tacgtgtgga tcatgccccg tccctcgact tgcaaagcca
     2701 cccttttcag cgatacgacg ccgtcttggt aggaatcgat cttccccacg ccaatgggct
     2761 cgagccagtc gaaaaacttc gcacttccgg cgtcaccgta cccatcgtcg ccctgacgac
     2821 ttccacgtgc ccccacgacc acaatcggtg cttggaatcc ggatgcacgg ctgtcgtaac
     2881 caaaccgatc gaacggacca cgctgctcca gcgactcgat gatctcatca gcacgacgga
     2941 agatcgtcgt gtcatgtccg tgcttccacc ggacatcgcc attgcggatc cattaccatc
     3001 gacgctcgag accgaacctt ccgtgcgtca gattttgccg caactcgtta tgcgaatgcc
     3061 caggctgatc aagcatatcg cgcaagcgat acgacgactc gatctgccca cggccgcgag
     3121 gaccgcacgt tccttggggg cgaccgctgg taattgcgga ttcgagtcct tgtcgaaagc
     3181 cgcccacgcg ctagctgatg cttgcgagca tcctcccatc gataccgatg tccttgggga
     3241 tcgcctcgaa cagctcgaaa acatggcgaa gcgtgttcag gccgcctatc cggacttcat
     3301 caaaccctcc gaataatcgc cttactcgtt gtcgaatcag ccctccgagg tctttccctc
     3361 atcatctccc cctttccgtg acaactaccg caacatcggc cctcgcttcg tatcgagccg
     3421 attcgatacc aacttgccac gcgctgtact aagtcttcgt ggtctgaatt cgcccccacg
     3481 caaaccgagt agctcatgag ccaagaccgg atttccgtcg tccttcacgc ccatttttat
     3541 caacctccgc gcgacaaccc ttggactgaa tcgatcgatc gcgaagcttc ggccgctccg
     3601 tttcacaact ggaacgagcg tatcgaagcg gaatgctac
//
LOCUS       DAOSOR010000585         3617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_25779_length_3617_cov_4.082538, whole genome shotgun sequence.
ACCESSION   DAOSOR010000585 DAOSOR010000000
VERSION     DAOSOR010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25779_length_3617_cov_4.082538"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            557..1339
                     /locus_tag="PK710_22025"
     CDS             557..1339
                     /locus_tag="PK710_22025"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT12473.1"
                     /translation="MEQARNYVCLSCSAPVPLGHKFCGRCGTGVPPEILDARTLFFSD
                     MQNPTKAKLVLIRGEGAEGLSYHLRAEEHLAGRSGNLQFPDDPFISERHANFFYRNDK
                     LVVRDEASVNGVYIRVRGSVEVSMGDTFLAGEQMFRIEANNVSNDPPADDGTYFYASP
                     KHPGPFRIVQVFQGGISGMVVCARASSLQIGREGGDLNFPGDPYMSGTHCRLEDNHGK
                     LTLTDLNSRNGTYVRIVGERELEHGDYLFLGRKLLRVEMNTN"
     gene            complement(1370..2488)
                     /locus_tag="PK710_22030"
     CDS             complement(1370..2488)
                     /locus_tag="PK710_22030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOT12474.1"
                     /translation="MAWLVRLLPWLGDESTFPPERKVCGLTLALRIALAAQAGGACAV
                     ELSPKVAFLLPLLEDPRLRIPVRMADENSNVALRVDMPWNLVIHRDLLKDLASSGATG
                     IRNLFEQPHAFHPPFGFEPIVVSDRNSKLRAERALLRSLRKVQDGWTSTYLNRYISLF
                     ITRWLTAIPIRPNQLSLAILGVGLCGAYLASRGTYGAMLLGALLFQAQSVLDGCDGEL
                     SRVTYRGSHLGEWLDTIGDDLTNYGFFAGLAWGLHSVSGSWWYLAAGAVVVGCGVITS
                     TIEYLYLIRIGSGDLLKYPLGIGKAPGADAKEAKRKGIVDAISPLFKRDSFVLLTLVG
                     TAVGLAGPFLFVFAAGGIGIFVAVLQAEFRMAKERKMR"
     gene            2513..>3617
                     /locus_tag="PK710_22035"
     CDS             2513..>3617
                     /locus_tag="PK710_22035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12475.1"
                     /translation="MGNLALFTPIVSVIGFGLLSVACGGEGESRFGASVDSQFVVPDS
                     LEALVAENWLDHPWPSDFRRSTDGSVRIAGFDNPRSVVLIDTYLTALDKAIDGFSPVA
                     AGALRFTGPIDPSTLPSDPKAAMAPNASVQLVDVDDRSTECGKRQWLSLYWRQEAGVW
                     WRPNTLAFMPTMGFPLRPKTRYALVVTDRVTAQDGSRLGVAEDLLEVLGLREATDRTR
                     GLRDAWAPSIEVLEKEGILRDHIVHLTVFTTSDPTMELQAIADHARSREPAPEVDDAW
                     ELKEQTPDFDVYEGSYGPVPDYQRGEPPFEVVGGGFEINAEGVPVVQREYHARFSLLV
                     PNAQACPMPAEGYPVVMYAHGTGGDYRSVVGVTG"
BASE COUNT          784 a          986 c         1079 g          768 t
ORIGIN      
        1 gtccggcttg gcgcatgaac cacaccggag tgcagtcaac ggactcaccg cggcaagcgc
       61 gtaaaaagcg gtcattcatg gggcgcgatg gtatcatggg cgggatgaaa aggcgggagg
      121 aaggaagcca tcagcggatg ctttcgagcc tggcgatgcg gatggagcat agagcttgac
      181 cctggtggga agcgatggct acaaatcgag tttgaaggta gtgtgtcggc cttggtgtgt
      241 tggtgaaggt tgacgcaagc cgcacgaaac gtgtggatgg tatgaaaagc cggcgatgga
      301 attcgagata ggagtcgttt gtgggcgatg cgatcgcttc agcctgatgg gcacggggcg
      361 gtgtgtgtgc ggccatgatc tcactctatt caagacctcc acctcccctt cccagcctgc
      421 gggaagttca aaatcggggt cgatggcggt tcgtcgccct accccacgag aagaagtggt
      481 acgttctcga ccggcagagg tattgtcccg cccggcccaa gccagccgtt ttgcgcatct
      541 ttctcaggag gagctcatgg agcaagcccg caactacgta tgcctgtcct gctcggcgcc
      601 tgtcccgttg ggacataagt tctgtggccg ttgtggaacg ggggtacccc ccgagatcct
      661 cgatgcacgc acgctgtttt tcagcgacat gcagaatccc acgaaagcca agctcgtgct
      721 catccgcgga gagggggccg aagggctttc ctatcacttg cgagccgaag agcacttggc
      781 cgggcgcagt ggcaatcttc aattcccaga tgatcccttc atctccgaac gtcacgccaa
      841 tttcttttat cgcaacgaca agctcgtggt tcgcgacgaa gcctcggtca acggggtcta
      901 tattcgtgtg cgaggaagcg tggaagtatc catgggagac acgtttctcg cgggcgaaca
      961 gatgtttcgc atcgaggcga acaacgtgag caacgatccg cctgccgatg atggaacgta
     1021 tttttacgcc tcaccgaaac atccggggcc cttccgtatc gtgcaagtct ttcaaggagg
     1081 catttcaggc atggtggttt gcgcgcgagc ctcctcgttg caaatcggcc gtgaaggcgg
     1141 agacctcaac ttccctggcg acccctatat gtccggaacc cattgccgtc tcgaagacaa
     1201 ccacggcaaa ctcaccttga cggatctcaa cagtcgcaac ggcacgtatg ttcgcatcgt
     1261 tggcgaacgt gaattggagc atggggatta tttgttcctc ggccgcaagt tgttgcgcgt
     1321 cgaaatgaac accaactgat cgccaatcgt tgctgaacct atagcgtcat caccgcattt
     1381 ttcgttcctt ggccatccga aattcggcct gcaacacggc tacgaatatt ccgatgccgc
     1441 ctgccgcaaa gacaaataga aatggtcccg ccaatcccac ggccgtgccc acgagcgtga
     1501 gcaacacgaa actatctcgt ttgaacagcg gtgagatggc atcgacgatc ccttttcgtt
     1561 ttgcttcttt tgcatccgcg cccggagctt ttcctatgcc gagggggtac ttcagaaggt
     1621 ccccggaacc gatgcgaatc aagtagagat actcaatggt gctggtgatc acaccgcatc
     1681 cgacgaccac ggcgcccgct gcaagatacc accacgaccc cgagaccgaa tgaagccccc
     1741 acgcaagtcc agcgaagaaa ccatagttgg tgagatcatc gcctatcgta tccaaccatt
     1801 ctccaagatg ggaaccgcgg taggtgactc tcgacaactc cccatcgcac ccatcgagca
     1861 cgctttgcgc ttgaaatagc agcgcgccaa gcagcatcgc gccgtaagtc cctcgcgaag
     1921 ccagataagc tccgcacaac cccacgccaa gaatggctag cgaaagctga ttggggcgaa
     1981 tcggaatcgc cgtgagccac cgggtaatga acagggaaat atatcgattc agataggtcg
     2041 aggtccatcc atcctggact ttgcgtaagg atcgcaaaag ggcacgttcg gcacgcagct
     2101 ttgagttacg atcggaaacg acgatgggct cgaagccaaa gggtggatgg aaggcatggg
     2161 gttgttcgaa aagatttctg attcctgtcg cgccgctcga ggccaggtct ttgagcaaat
     2221 cgcgatgaat cacgagattc catggcatat cgacccgtag tgcgacgttg gaattttcat
     2281 ccgccatgcg aacgggaatg cgaagtctgg ggtcctcgag caaaggaaga agaaaagcga
     2341 ccttgggcga cagttccacc gcgcaagctc cgcctgcttg tgccgccagc gctatccgca
     2401 gggcaagcgt caggccacat acctttcgtt caggaggaaa tgtcgattca tcacccaacc
     2461 acgggagcag ccgaacgagc catgccatcg ccgccgatgt acaacaatgg gcatgggaaa
     2521 tcttgcgttg ttcacaccca tcgtgagcgt catcggcttc ggcttgcttt ctgtcgcttg
     2581 cggaggcgaa ggagaatcgc gattcggtgc ctcggtcgat tcacaattcg tcgtccctga
     2641 ttccctggag gccttggtgg ctgaaaactg gctcgatcac ccatggccga gtgattttcg
     2701 tcgctccacg gacggctccg ttcgcatcgc tggctttgac aatcctcgtt cggtagtgct
     2761 gatcgacacc tatcttacgg cgttggataa agccatcgat gggttttctc ccgttgccgc
     2821 gggggctttg cgattcaccg gtcccatcga tccatccacg ttaccttcgg atccaaaggc
     2881 agcgatggcc ccgaacgcct cggttcagct cgtggatgtg gacgatcgct ccaccgagtg
     2941 cggaaagcgt caatggctgt cgttgtattg gcgacaggag gcgggagtat ggtggaggcc
     3001 caacacgttg gcgttcatgc ccaccatggg ttttccgctt cgtccgaaga ctcgttatgc
     3061 cttggtggtg accgatcggg tgacggctca ggatggttca cgtcttggag tggccgagga
     3121 tttgcttgag gttctgggat tgcgggaagc gacggatcga acgcgagggc ttcgggatgc
     3181 gtgggcgcca tcgatagagg tattggaaaa ggaagggatt ttgcgcgatc acatcgtaca
     3241 tctcacggtg tttacgactt cggatccgac gatggagctg caagccattg cggatcatgc
     3301 gcgcagtcgg gagccggccc ctgaagtgga cgatgcatgg gagttgaagg agcaaacccc
     3361 ggacttcgat gtatacgagg ggagctatgg gccggttccg gattatcaac gaggtgaacc
     3421 gccctttgaa gtcgtcgggg gaggattcga gatcaacgcc gaaggagttc ctgtcgtaca
     3481 acgcgagtat catgctcgat tttctctgtt ggttcccaac gcgcaggcct gcccgatgcc
     3541 ggccgagggc tatccggtgg tgatgtacgc gcatgggacg ggaggagatt atcgatccgt
     3601 ggtgggcgtg acgggca
//
LOCUS       DAOSOR010000586         3612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_25827_length_3612_cov_3.735732, whole genome shotgun sequence.
ACCESSION   DAOSOR010000586 DAOSOR010000000
VERSION     DAOSOR010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25827_length_3612_cov_3.735732"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..>3203
                     /locus_tag="PK710_22040"
     CDS             169..>3203
                     /locus_tag="PK710_22040"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOT12476.1"
                     /translation="MNLSDLAVRRGVTFTMVFLVIVGFGLFSLSRLQLDLYPDITLPS
                     VVVITNYTGASPEDIETLVTRPIEGAVAAVKDVEEIRSTSKQGVSLVEVKFDWGKDMD
                     QAETDVRRKLEMIEALLPQDADDSIVFAFDPSLQPVVMLMVTGPYPLDELRRIAENDL
                     EPRIARLPGIASAEVAGGLEREVHVELDPTKIAAFGLDVNNVIGAVYRENSQIPGGAL
                     EQGTLDFTIQTEGKYSNVEQIGEVVVGMKPTDTGPVPIRLKEVARVVDTFYESQRVLE
                     VDGEPTVWLLVRKQSGANTVRAAEGVIEALPNLSRSVAAELNFKVIFNQADFINESLG
                     NLSSTGLLGVGITFLVLLVFLRNMRSSLIVASAIPLSVVATFSIMDRANMTLNTLSMA
                     GLALAIGMLVDNAIVVLENIFRLRQEGMNAWDAAIHGAKGVSLAVTASTLTTVAVFVP
                     VLFVPGIAGVLFQDMSITICFSLMVSLVVALTFIPLASSRLLGTERATRLLEKAAKND
                     PLRRVQHHYGRALDWVLAHRWVTLVGLVGALAITALLAAAMPTEFVPKDDQSLLFVTI
                     ETPIGNNLQQTYKMVKEAEEHVKKIIPEDARKLVALDVGVGKGFVSIFSKGVHAGLLR
                     VPLVKPDQRTKSQAQYEAEVREDLRGIPGIKPTVSMPFNLMGDGDIAVEIRGHDLDMS
                     RNLGLDLVEKLKTMPDLAEVVFSMDDQKPEVRVRFDRLKMAELGISAAAVGNVISTYF
                     MGRTAGRYAEGGDEYDIVVRYAKAHRRDVDELRKMPIATPSGQVVPLDNIASVDIGLG
                     PVDITRLDQERITTLKIYLHDDWVDSKGKNQKKDLGGTIGRVDEYLKNYPWPPGFSYE
                     IGGSAEDFLTSFRYLGWALLVSVLLVYIVMASQFESFRQPFIIIFAVPLAAIGVVLMF
                     TVTRSVMNISSLVGVIMLAGIVVNNGIVMIDAANQLRDEGKGRLEAIAIASRIRLRPI
                     ILTSATTVLAMLPLALGIGEGAAAWSGMAKAVIGGLTVS"
     assembly_gap    3204..3213
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3415..>3612
                     /locus_tag="PK710_22045"
     CDS             3415..>3612
                     /locus_tag="PK710_22045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12477.1"
                     /translation="MHSMGFGWMRLILLGGWVRAGAWARNIKARRVHAANLGLAGLEC
                     GLMLDDGTVLLVMGVLGYAACL"
BASE COUNT          747 a          947 c         1081 g          827 t
ORIGIN      
        1 gccagcgggt cgttcattgt ggagcgagaa aaggctcact ttaccaggtg ctcgacggcc
       61 tcgccgaagg agaccgaatc atcgtgcggg gacaacacgg tcttcgcgat ggccaacgtg
      121 tcgaaattgt cgacgcgaaa tccgaatagg cgtgagcgag gcataccgat gaacctatcc
      181 gaccttgccg ttcgccgtgg tgtcacgttc acgatggttt tcctcgtcat cgtgggcttt
      241 ggcctgtttt ctctttctcg tctccaactc gacctttatc ccgacatcac gctgccttcc
      301 gtggtggtca tcaccaacta cacgggagca agcccagaag acatcgaaac gctcgtcaca
      361 cgacccatcg aaggcgccgt ggccgcggtc aaagacgtcg aggagatccg ttccacttcc
      421 aaacaggggg tatccctggt cgaggtcaag ttcgactggg gaaaggatat ggatcaggcg
      481 gaaacggatg tgcgtcgaaa gcttgaaatg attgaggcct tgcttccgca agacgccgat
      541 gactcgattg tttttgcgtt cgatccatcg cttcagccgg tggtgatgtt gatggtcacg
      601 ggcccgtatc cgttggatga gttacgacgg atcgcggaaa acgacttgga accgaggatt
      661 gcgcgcttgc caggcattgc ttctgccgag gtggctggag ggctcgagcg agaagtgcat
      721 gtggaactcg atcccacgaa aatcgcggct ttcggtttgg acgtcaacaa cgtcatcggc
      781 gcggtttacc gggaaaattc gcagattcct ggtggcgcgc tggaacaggg aaccctcgat
      841 tttaccatcc aaaccgaggg aaaatacagc aacgtcgaac agatcgggga agtcgtggtc
      901 ggaatgaagc cgacggacac ggggcccgtg ccgatccggc tcaaagaagt cgctcgggtg
      961 gtggacacgt tttatgaaag ccagcgagtg ttggaggtgg atggggaacc tacggtgtgg
     1021 ctgctcgttc gaaagcagtc tggcgccaat acggttcgcg ccgccgaggg agtcatcgag
     1081 gcgttgccca atctcagccg ctcggtagcc gccgagctga acttcaaggt catcttcaac
     1141 caggcggatt tcatcaatga atcgctcggg aacctatcct ccacgggact gttgggcgtg
     1201 ggaatcacct ttctcgtcct gctcgtcttc ctgcgcaaca tgcgaagctc tttgattgtc
     1261 gcttccgcca ttcccttatc cgtggtggct accttttcca tcatggatcg agccaatatg
     1321 acgctcaaca cgctatcgat ggccggattg gcgctggcca tcggtatgct cgtcgacaac
     1381 gccatcgtgg tgctcgaaaa tattttccgt ctccgacagg aggggatgaa cgcctgggat
     1441 gctgccattc acggtgccaa aggggtgagt ttggcggtga ccgcctccac cttgaccacg
     1501 gtggcggtat tcgttccggt gcttttcgtt ccgggaatcg cgggggtgtt gttccaagac
     1561 atgagcatca ccatctgttt ttcgttgatg gtttccctgg tcgttgcctt gacgttcatt
     1621 ccgcttgcga gttcgagact tttgggtacg gagcgggcca ctcgtttgct ggaaaaagct
     1681 gctaaaaatg atccgttgcg gcgtgtccaa caccattacg gtcgcgcctt ggattgggtg
     1741 ctggcgcatc gatgggttac gttggtgggt ttggtgggcg cgttggcaat caccgcattg
     1801 ttggccgcgg ccatgccgac ggagttcgtt cccaaggatg accaatcgtt gctctttgtc
     1861 accatcgaaa caccgattgg caacaacctt cagcagacct acaagatggt caaagaagcg
     1921 gaagaacacg tcaaaaaaat cattcccgag gatgccagaa agctcgtggc tctcgatgtg
     1981 ggtgtaggaa aaggcttcgt ttcgattttc tccaaaggcg ttcatgccgg cctattgcgt
     2041 gttccgctcg tgaaaccgga ccaacggacg aagtcgcaag cgcagtacga agccgaggta
     2101 cgtgaggatt tgcgagggat tccaggcatc aaacccacgg tttccatgcc gttcaacctg
     2161 atgggcgatg gcgatatcgc ggtggagatt cgggggcatg acctcgatat gtcgagaaat
     2221 ctaggactcg acctcgtgga gaaactcaaa accatgcccg atctcgcgga agtcgtcttc
     2281 tccatggacg accagaaacc cgaggtgcgt gtgcgattcg accggctcaa aatggccgag
     2341 ctaggcatct ccgccgccgc tgtcggtaac gtcatttcca cctacttcat gggtcgtacc
     2401 gcaggccgct acgccgaagg gggtgatgaa tacgatatcg tcgttcgtta cgccaaagcg
     2461 catcgtcgtg atgtcgatga attacggaaa atgccgatcg ccacgccgag cggccaggtc
     2521 gtccccctgg acaatatcgc cagcgtcgac atcggtctcg gccccgtcga tattacccgt
     2581 ctcgatcaag agcgcatcac cacgttgaaa atctatctgc acgacgattg ggtggattcg
     2641 aaggggaaaa atcaaaaaaa ggacctgggt ggaacaatcg gccgtgtgga cgaatacctc
     2701 aaaaattatc cttggccgcc tggttttagc tacgagatag gcggttcggc ggaagacttc
     2761 ctcacttcgt ttcggtatct gggctgggcg ctgctcgtct ccgtgttgct cgtgtacatc
     2821 gtcatggcga gccaattcga gtctttccgc caacccttca tcatcatttt tgccgttcct
     2881 ctcgccgcca tcggcgtggt gttgatgttc accgttaccc gcagcgtcat gaatatctcc
     2941 tcgttggtgg gagtcatcat gctggcgggc atcgtggtca acaatggcat cgtgatgatc
     3001 gatgcggcca atcaacttcg cgacgaaggc aagggaaggc tggaagccat tgcgatagca
     3061 tcgcgaattc ggttgcgtcc catcattctc acgagtgcaa cgacggtgtt ggcgatgctt
     3121 ccgcttgcgc tggggattgg ggaaggggct gcggcctggt caggcatggc gaaggccgtc
     3181 atcggtggtc taaccgtgtc tatnnnnnnn nnncacgttc ttcgccagca aaaaacagga
     3241 actcaaagct cgcgagcgga tctctcgggt gagtggggct catgcccctt ccgagtgagc
     3301 aaggggagca ccgaccggaa tcatccagcc cctatcgaac cggcgtatga cccagcggga
     3361 atacgggaaa caggctttgc gtacggtatg gcgaaacaat gttggatgct gcccatgcat
     3421 tcgatgggtt ttggctggat gaggctcatc ttgttggggg gttgggtacg ggctggcgct
     3481 tgggctagaa acatcaaggc caggagagtc catgccgcga atctgggttt ggccgggctt
     3541 gaatgtggct tgatgctcga tgacggaacg gtgttactgg tcatgggcgt tttgggttat
     3601 gccgcttgct tg
//
LOCUS       DAOSOR010000587         3601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_25921_length_3601_cov_4.344050, whole genome shotgun sequence.
ACCESSION   DAOSOR010000587 DAOSOR010000000
VERSION     DAOSOR010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25921_length_3601_cov_4.344050"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..427
                     /locus_tag="PK710_22050"
     CDS             158..427
                     /locus_tag="PK710_22050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12478.1"
                     /translation="MRCSGCRLGGWEDGRKVFRATARRKPCARASDIEAVATGAVQRN
                     EESVLQALQADSTIAFRFWSPQALDALGVLLGPNSGPYVPIGGVG"
     gene            548..862
                     /locus_tag="PK710_22055"
     CDS             548..862
                     /locus_tag="PK710_22055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12479.1"
                     /translation="MLIADYMKVGTAIDLWPWRQVACGCPEIIGWMVLDKRNPSSAFG
                     RICARCTLSFETRFPDAKSSKPSRSSSVRLMMYRVAIPNMEHTSGGMESEYAIKSMET
                     EH"
     gene            1065..3167
                     /locus_tag="PK710_22060"
     CDS             1065..3167
                     /locus_tag="PK710_22060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12480.1"
                     /translation="MSLPFPVSRFLFSVSCNFRAACGLFAAGLLLSCGTTEEPSVDET
                     VTMAASVESEPLIGDGDIPSRPPVAMSWGMTDGSHVAGKFDPFRVTLRNERMEKVEVA
                     LSMVGTGLDQRMVKVDVGTFHLKDLESHQIDIPIESLPIQSVGAPAQIELTATCSGAG
                     WMLELPAERVYVQFTPDFSKVHASTTSTWAPVALGLSGGDVSSKAFQTLLQDPMSAVR
                     ADASKGGGDPDAVDLERAKSLYESLVRPVLEPVGRYVDDTGTLVDLIEPRTGGGMLPV
                     TREQAALLETVLIHAAPAVPTNPAPLDFPVIGRTVRMCAKWGVQYNDSGLGEDYLNGV
                     GHQLYPARFAKGQIRRLTPNVQDFWAGQFDFDGCAPTMFLPYAQYYLYVEPNFTTTNN
                     TKFRVTGDNASQFWAWWLPFTIGSLPPVLALSTANHEASRIGAVVSTLLSNWDPGVAV
                     VSNKNYRLVASTTDCEQTGAFSRGEYVCYGFDPGGGPHTTISKIVIAHEIAHSLDASG
                     SGTPYNTYYWELTGVPACRCDHVLTANQLHCIQSWEQAGGAFIEGFAQFYAAKVFNEW
                     RHTDCVFAYYKEFRQDNGVVLMPPVAMSCRTNHRWMKNHCDYSAQSGGVEMDWMTYFW
                     NVHAPQEYGNKRISMQEYHLIKRKSCTGSTGSLCSSAHQLDFETWRVGALDYFGSSSN
                     LKFQHVRDMGYLHGVNRY"
     gene            3178..>3601
                     /locus_tag="PK710_22065"
     CDS             3178..>3601
                     /locus_tag="PK710_22065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12481.1"
                     /translation="MRSHLVILCSLVIMGCSNEPTVTAPNPIHRADATPPAAPVEASA
                     EAHLDVDKEPDDPNVLWREGDCSHPLVEKRCEHGWCAIPSGCFVIGSPEDELGRAPYM
                     EGQVPVTITRPFHFGQFEVTQELWTSLGLPNPTAENEYC"
BASE COUNT          723 a         1028 c         1079 g          771 t
ORIGIN      
        1 gcgggcgtct cgcccgcatg gtctccatcg tcacccgcgc tgccgtatcg ccagtgttgt
       61 ttgtgcgcaa cgagccccgc ctggaccgga ttggactcga tgtagttgcc cttgtcctac
      121 accgaccatc ccgtttgaca gcaatatgac cgggcacatg aggtgttcag gttgccgtct
      181 tggaggatgg gaagatggcc gcaaagtatt ccgcgcaaca gcgcgccgaa agccttgcgc
      241 gcgagcctcc gacatcgaag cggtggccac tggtgccgtg cagcggaacg aagaatctgt
      301 tttgcaagct ttgcaagccg actccaccat agcgtttcgc ttctggtcgc cccaagcgtt
      361 ggatgccctt ggggtattgc ttgggccgaa ttcagggccg tatgtaccca ttggcggcgt
      421 aggatagggg catcttttcg agtcgatatc aaaccgcgcg agcgctttct ggctgaggtg
      481 atggccgcgg ctgtaccagt cgccgggtga ttggaccgcg cggttgccgc tacgcttgcg
      541 cgcagcaatg ctcattgccg attatatgaa agtgggtacg gcgattgatc tgtggccgtg
      601 gcgtcaggtc gcctgcggat gtccggagat tattggttgg atggtgcttg acaagcgcaa
      661 tccctcgtcg gccttcggca ggatctgcgc ccgctgcacc ttgagcttcg agactcgctt
      721 ccctgatgcc aagtcttcca agccaagccg ttcttcctct gtgaggttga tgatgtaccg
      781 tgtcgccatc cccaacatgg aacacacatc aggcggaatg gaaagcgaat acgccatcaa
      841 atcgatggaa acggagcact aaaataaagt aacaacgatt ccaaagggtg gttcgaggag
      901 gatgcagagc gcttgtgttc catgttcaaa tgaaaacttt tcagattacg gtcttttggt
      961 gtgtgttatt tgctagatgc attgaatttg ttattgctac ataatacgcg aaagtgggcc
     1021 ctgtgttgga agtaaaatgt ttctgctcgc aaaggaggaa agaaatgtca ctaccgtttc
     1081 ccgtttcccg tttcctgttt tctgtttcct gtaatttccg cgcggcgtgt gggttgttcg
     1141 cggctggttt gctcctgagc tgcggaacga ccgaggagcc ctccgttgac gaaaccgtga
     1201 caatggctgc cagcgtggaa tccgaaccgc taataggcga tggggatatc ccaagtcggc
     1261 ctcccgtcgc gatgagctgg ggcatgaccg acggttccca tgtcgcaggg aagttcgacc
     1321 ctttccgggt caccctccgg aacgagcgga tggagaaggt cgaggtcgcc ctgtcgatgg
     1381 tcgggacggg attggatcaa cgcatggtca aggtggacgt tggtaccttc cacctcaagg
     1441 atctcgaatc ccatcagatc gatattccca tagagagcct tccgattcag agcgtcggag
     1501 cgccggctca aatcgaactc acggcaacct gctcgggtgc cgggtggatg ctcgagttgc
     1561 ctgccgaacg agtgtatgtc cagttcactc cggacttctc caaagtccat gcgtcgacga
     1621 cgagcacatg ggcacctgtg gcattgggct tgtcgggagg ggacgtgtcg agcaaggcgt
     1681 tccagacgtt gctccaggat cccatgagcg ccgtcagggc ggacgcttcg aaaggaggcg
     1741 gtgaccccga cgcagtggac ctggagcgag cgaaaagtct ctatgagtct ttggttcgac
     1801 ctgtgctcga gcctgtgggc cggtacgtcg acgatacggg cacgctggtc gatctcatcg
     1861 agccgcgaac tgggggaggg atgttgcccg tgacgcgcga gcaagcagct ctgttggaga
     1921 ccgtgctcat tcacgccgcg ccggcggttc ccaccaaccc cgcgcccctc gactttccgg
     1981 tgattggccg taccgttcgc atgtgcgcga agtggggcgt ccagtacaac gattccggtc
     2041 tgggtgaaga ctacctcaac ggggtggggc accagctcta tccggctcgc ttcgccaagg
     2101 gacagatccg tcgccttacg ccgaacgtgc aggacttctg ggctgggcaa ttcgacttcg
     2161 atggatgcgc ccccacgatg ttcctcccgt acgcgcagta ctacctgtac gtggagccga
     2221 atttcacgac gaccaacaac accaagtttc gagtgacggg agataacgcg tcgcagttct
     2281 gggcgtggtg gcttcccttc accatcggtt cactccctcc ggtcctggcg ctttccacgg
     2341 cgaaccacga agcgagccgc attggtgcgg tggtctccac gctgctgagc aactgggatc
     2401 cgggcgtggc ggtggtgtcg aacaagaact accggctggt tgcgtcgacg accgattgcg
     2461 agcaaacagg cgccttttcg cgcggagagt acgtttgcta cggcttcgat cccggtggtg
     2521 gtccgcacac cacgatttcg aagattgtca tcgcgcacga gatagcacac agccttgacg
     2581 cctccggctc cggaacaccc tacaatacct actactggga gctgactggt gtgcccgctt
     2641 gtcgctgtga ccacgttctc accgcgaacc agcttcattg catccaatcc tgggagcagg
     2701 cgggtggtgc cttcatcgaa gggttcgccc agttctatgc cgcgaaggtc ttcaacgaat
     2761 ggcgccacac ggactgcgtc ttcgcctact ataaagagtt ccgacaggac aacggcgtgg
     2821 tactgatgcc acccgtggcg atgagctgtc gaaccaacca ccgatggatg aagaaccact
     2881 gcgactactc cgctcagagc ggcggcgtgg agatggactg gatgacgtac ttctggaacg
     2941 tgcatgcgcc ccaagagtac gggaacaaac gaatctcgat gcaggagtac catctgatca
     3001 agaggaagtc ctgtacggga agcacaggga gcctctgctc gagcgctcat cagctcgact
     3061 ttgagacatg gagggtcggt gctctcgact acttcggatc atcatccaac ctcaagttcc
     3121 aacatgtgcg ggacatggga tacctgcacg gcgtgaatcg atactgaggc aagcatcatg
     3181 aggagtcatc tcgttatcct ctgctcgctg gtcatcatgg gttgctcgaa cgagcctacg
     3241 gtgacggcac ccaacccgat tcaccgagct gacgccacac cgcctgctgc tccagtggaa
     3301 gcaagcgcgg aggcccacct cgacgtcgat aaagaacctg acgacccgaa tgtgctttgg
     3361 cgcgagggag attgctcgca ccccctcgtg gaaaagcgct gcgagcacgg ttggtgcgct
     3421 atcccgtcgg ggtgcttcgt catcggctcc ccggaagacg aacttggccg ggctccctac
     3481 atggaaggac aggttccagt caccatcacc agaccctttc acttcgggca gttcgaggtg
     3541 acgcaggaac tctggacgtc cctcggcttg ccgaatccca cggcagagaa cgaatactgt
     3601 t
//
LOCUS       DAOSOR010000588         3587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_26074_length_3587_cov_3.389581, whole genome shotgun sequence.
ACCESSION   DAOSOR010000588 DAOSOR010000000
VERSION     DAOSOR010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26074_length_3587_cov_3.389581"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1747)
                     /locus_tag="PK710_22070"
     CDS             complement(<1..1747)
                     /locus_tag="PK710_22070"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOT12482.1"
                     /translation="MMTAKIVWTKIDEAPALATYSLLPIVQAYTKGTGVIVETRDISL
                     TGRIIANFPDRLRDDQKIPDHLAQLGELAKTPQANIIKLPNISASIPQLHAAIRELQD
                     KEYDIPSFPEEPKNEAEKALQTRFAKVLGSAVNPVLREGNSDRRAAASVKSFAKKNPH
                     KMMQPWPSSGSKTRVVHMQENDFYGSEKSVTMSKADVLRIEFVAEDGSVTVLKEQLPV
                     QMGEVVDATVMNVASLRAYYADTIDQARKQGVLLSLHLKATMMKVSDPILFGHAVSVF
                     YADALDKHADLLREVGANVNNGLADVLEKIERLPADKKAQIEADIQAVSERQPALAMV
                     DSRKGITNLHVPNNVIIDASMPNVVRDGGKMWNKHDQLQDTWAMIPDRCYATIYQTII
                     EDCQQRGQFDPATMGSVSNVGLMAQKAEEYGSHDKTFEAKGRGVIRLVDSAGQTLLEQ
                     SVQKGDVFRACQVKDAPIRDWVRLAVRRAKASGAPAIFWLDERRAHDAQVIAKVKAYL
                     PDHDTTGLDIRIMRPDDAMRFSLERIRRGQDTISVTGNVLRDYLTDLFPILEFGTSSR
                     MLSIVPLMQGGGLFET"
     gene            complement(1933..2592)
                     /locus_tag="PK710_22075"
     CDS             complement(1933..2592)
                     /locus_tag="PK710_22075"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOT12483.1"
                     /translation="MKTSKYVGWMMGLVLVGALAGCDNEPAKGAAKAVTSAVQPVKEE
                     PAKEATTSTVKYKFDQAESKVQWVGAKITGKHDGGFGTFEGTIDLVDADPTKSRVSVE
                     ITMDSLTADAEQLVGHLKSKDFFEVETYPKSRFTSTAVVAGGDGGASHTVTGNLELHG
                     VTKSITFPATITVQGDTVAVKAEFAINRKDFGIVYPGKPDDLIKDDVLLRLNISAKKS
                     Q"
     gene            complement(3050..3442)
                     /locus_tag="PK710_22080"
     CDS             complement(3050..3442)
                     /locus_tag="PK710_22080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12484.1"
                     /translation="MGDLIGSLRGGSFQRWWAFGASVCVAMAGCGGIVAEPEEGQDSD
                     QEESSAPSTGGNASGTGKSAGAKGPGKTGDCQSCEGQPVCAFCLVQSYEATIICPIGK
                     KPSRSGCMSLGERYSSSDGQTVSCHYCR"
BASE COUNT          785 a         1148 c          856 g          798 t
ORIGIN      
        1 ccgtctcgaa caatccccct ccttgcatca acggcacgat cgaaagcatc cgcgaactcg
       61 ttccaaactc caaaatcgga aataggtccg tgagataatc gcgaagcacg ttgcccgtca
      121 ccgaaatcgt atcctgccca cgacgaattc gttcgagaga aaaccgcatc gcatcgtccg
      181 gtcgcatgat gcgaatgtcc aatcccgtcg tgtcgtggtc cggaagatac gccttgacct
      241 tcgcaatgac ctgagcgtcg tgcgcacgac gttcgtccaa ccaaaagatg gccggagccc
      301 ccgaggcttt cgcacgacga accgccagcc gcacccaatc tcgaattggc gcgtctttca
      361 cctgacaagc ccgaaataca tcacccttct gcaccgattg ctcgagcaac gtctggcccg
      421 cgctatccac caggcgaatc actccccgac ccttggcctc gaacgttttg tcatgggagc
      481 catattcctc cgccttctgc gccatcaatc ccacgttcga cacactgccc atcgtcgctg
      541 gatcgaattg tccacgctgc tgacaatcct cgatgatcgt ttgataaatc gtcgcatagc
      601 aacggtcggg aatcatggcc caggtgtctt gaagctggtc gtgcttgttc cacatcttgc
      661 cgccatcgcg aacgacattg ggcatggaag cgtcgatgat gacgttgttg ggcacgtgta
      721 agttagtgat tcctttacgg gaatcgacca tggccagcgc cggttgtcgc tcggaaacgg
      781 cctggatatc agcctcgatc tgcgcttttt tatccgccgg aagtcgctcg attttctcca
      841 gaacatccgc gagaccgttg ttgacgttgg cgcccacctc acgaagcaga tccgcatgtt
      901 tgtccagagc atcggcataa aaaaccgaca ccgcatggcc aaacaagatg ggatccgaca
      961 ccttcatcat cgtcgccttc aagtgcaaac tcagtagcac cccctgtttt cgcgcctggt
     1021 cgatcgtgtc cgcataatac gcgcgcagcg aggccacatt catcaccgtc gcgtcgacca
     1081 cttcccccat ctgcaccggc aactgttcct tgagtacggt gaccgaacca tcctccgcca
     1141 cgaattcgat gcgaagcacg tccgccttcg acatggtgac ggacttttcg ctgccgtaaa
     1201 aatcgttctc ttgcatgtga acgacacgag ttttcgaacc cgatgacggc cacggttgca
     1261 tcatcttatg cggatttttt ttcgcaaacg acttgaccga agcagcggcg cggcgatccg
     1321 agtttccctc ccgcagcacg gggttgacgg cggaaccaag gaccttggca aagcgcgttt
     1381 gtaaggcttt ttccgcctcg tttttcggct cctccggaaa gctcgggatg tcatattcct
     1441 tgtcttgaag ctctcgaatg gctgcatgga gctggggaat cgacgcgctg atgttgggaa
     1501 gcttgatgat gttggcctgc ggcgtcttcg ccaactcacc aagctgcgca agatgatcgg
     1561 gaatcttctg atcgtctctc aaacggtccg gaaaattggc gatgattcgt ccggtaagag
     1621 agatgtcacg cgtttcaacg atgacccccg tgcctttcgt gtaagcttgc acgataggga
     1681 gcaaagagta ggttgccaga gctggcgctt catcgatctt cgtccaaacg atcttcgctg
     1741 tcatcatcgt ctcctcgcgc tggaaagcag gctcccggct tgatggccgc acatcggaat
     1801 cgcgccgcat cccccatgga tgaaccacga ctcccacact cccgcaataa cccatcacaa
     1861 gagcgcccgt ttctcataat caagcgtgag gattgaatca agcagcgcga ggaagcgcga
     1921 aaaatgggag tattactgcg atttttttgc ggaaatattc agccgaagca acacgtcgtc
     1981 cttgatgaga tcgtcgggct tgcccggata cacgatcccg aagtccttgc ggttgatcgc
     2041 aaactccgcc ttcaccgcca ccgtatcgcc ctgcacggtg atggtggccg ggaacgtgat
     2101 ggacttggtc acaccgtgta actcgagatt gcccgtcacc gtatgcgatg ccccaccatc
     2161 accacccgcc acgaccgccg tcgaagtaaa cctcgacttg ggatacgtct ccacctcgaa
     2221 aaagtccttg cttttgagat gaccgacgag ctgctcggcg tccgcggtca acgagtccat
     2281 cgtgatttca accgatacac ggcttttcgt gggatcggca tccaccagat cgatcgtgcc
     2341 ttcgaaggtg ccaaacccgc catcgtgctt tcccgtgatc ttggccccca cccattgcac
     2401 tttggattcc gcctggtcga atttgtattt gaccgtgctg gtggtggcct ccttggcagg
     2461 ctcctctttc accggctgca ccgccgacgt caccgctttc gcagccccct tggcaggctc
     2521 attatcacag cccgcaagcg cccccaccaa caccaagccc atcatccatc caacgtattt
     2581 gcttgttttc atcatgcctc tcgagcgtca tgccacggta cgccatcgca ccggcagaaa
     2641 aggtttggga atgacccttc cgatgctcga ccactccatt tgctcgcacc gccgaggaga
     2701 caatctgcat atttcggtga tttgttgtcg tagcctcgta tttcaatgcc caattcccct
     2761 gatggccacg tcttcacccc gcaaaaaccc acggcaaacc gcgatcgccg atcgcagccc
     2821 cccgtcactt cacgtttttc cctcccctga accatgacgc catgattcat gaccgtttca
     2881 ccgcgcactg acacgcaccc ttttccccga aaggtcgcct cgccatgctt cgcataccct
     2941 agcccatcgg ctatggacaa atcttaccgt ttttcctgct ccttgaggga aaacgaaaac
     3001 ccaccttccc aggcaacgct aacatactga atttacaata atatttatgt catcgacaat
     3061 aatgacaaga aacggtctgc ccgtcggaag agctatagcg ctccccaaga ctcatgcagc
     3121 cggaacgcga gggtttcttg cctatgggac agataatcgt agcttcgtag ctctggacga
     3181 gacagaaggc acataccggc tggccctcgc aagactggca atcaccggtt tttcccggtc
     3241 ctttcgcgcc agcactcttg cccgtcccag aagcattccc gcccgtggaa ggagctgatg
     3301 attcttcctg gtcggaatct tggccctcct ctggctccgc gacaatcccg ccgcatccag
     3361 ccatagccac gcacaccgat gctccaaacg cccaccaacg ctggaacgaa ccaccccgaa
     3421 gcgaaccgat aaggtcaccc atgatccatc tccctgcgaa tagccttgcg attattgcaa
     3481 cctgcatgcc agcaaacggt tgcgaaatcg ctggtcgaaa ccgggtgatt ctcacgaacg
     3541 aacgtcaaat cagttagaat cctgacactc gtccacgcac cgaaagg
//
LOCUS       DAOSOR010000589         3572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_26219_length_3572_cov_3.686096, whole genome shotgun sequence.
ACCESSION   DAOSOR010000589 DAOSOR010000000
VERSION     DAOSOR010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26219_length_3572_cov_3.686096"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..1560
                     /locus_tag="PK710_22085"
     CDS             238..1560
                     /locus_tag="PK710_22085"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOT12485.1"
                     /translation="MLSTIPADGASGVAVDTNLVITFSSPMTPASLTAQTTAGACSGT
                     IQVSSNSFADCVAFKTAAPAMSGQDAVATLELETALTATGVYKLRVLGTAKDALGNDI
                     EPFLMTYGFTADINCLGQAIVISQAYGGGGNSGATYTHDFIELHNRGAMDVDLTGWSV
                     QFASATGTTWQVTPLTGTIAAGGYFLIQQAKGTGGTTSLPDPDAIGTSAMGAENFKIA
                     LVRNSTALTGACPNDPAIADFVGVGTANCFEGTVGAKLSNTTAALRKDQGCTETDDNA
                     ADFEALAPTPRNTKSPIVACSCSANETDEPYEIDYCVLQHPASVTVVKNTTTESIYGR
                     IFETGLTEAAGSADRVLAQLGWGPTSVNPSAQSGFQYVNATFNVQAGNDDEYAATISP
                     EVAGTYSYVYRFSIDGGAHWTYCDLDGAGSNASLSFDPALLGELTVTD"
     gene            1672..>3572
                     /locus_tag="PK710_22090"
     CDS             1672..>3572
                     /locus_tag="PK710_22090"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOT12486.1"
                     /translation="MLLEVPRIRSKAKTALVAAAVLAACGSPSELDTIGQRVSALSSN
                     LVIAEVYGGGGLNTGGTPAPYSHDFVVLFNRDSAPVNLKDFVLQYASPTNNFSNKIDL
                     PDHSLDAGQYFLIRLGTDNAAVGSPVPNPDLTPTAATNIGGTNGKLALARASKPLSGC
                     GGSANPCPAADIVDLLGYGTASQAEGTVLPALNHTSSAKRKLAGCQETDDNKEDFEIG
                     APTPRGTTSDKKDCAALPDGGAAGAGGGGGSGGSAGTAGSAGTAGSAGTAGSAGTAGS
                     AGTAGSAGAGDAGVVVTEGLVLAEIYPAGGNSGAVLKHDYVVLFNRGTQSVTLGGAVL
                     QYASAAGNFENTAKYTMSLPNESLLPGEYFLVQLDGGSNGGDLPTPDASGTLALGASA
                     GKVALALATAPLTGCGSSTTPCDSKDIIDLIGYGSTASQYEGSASASAPSVTKSMHRK
                     NGGCVDTNDNKNDIDVLDPNPRNRSTTKYDCSAVVDAGADVVADVVADVQQDAPQDVV
                     TDVVEAGADFDGVVIAEVYGGGGNSGASFTHDFVVLFNRGTEPVSLAGLSIQYASATG
                     SFSSSNNTVALPSAILAPGKYFLIQLALGTTGDGSALPTADLQATIAMSATAGKVALV
                     KSSALLDKCG"
BASE COUNT          740 a          990 c         1123 g          719 t
ORIGIN      
        1 ttctcgccca gcaccgattc caccttcggc cgcaccggaa tgacgcggta gccgtgcccc
       61 agcagtgtat gcatcgacta tgtctgccga tgtgacaatg gcaaaaaaga tggtgacgaa
      121 acggatatcg attgcggcgg aagttcatgc gctccgtgca aacccgaaca agcctgcctt
      181 caggataccg attgcgaaac cggacagtgt gtgagccaga agtgtggatg gacgaccgtg
      241 ctatccacga ttcctgcgga tggcgcgagc ggcgtggcgg tggacacgaa ccttgtgatc
      301 actttctctt cgccgatgac ccctgcttcc ctcacggcgc agactacggc gggggcgtgt
      361 tcgggaacca ttcaagtgtc ttccaatagc ttcgcggatt gtgtggcttt caagaccgcg
      421 gcgcctgcca tgagcggtca ggatgcggta gctaccctcg aactcgagac agccttgact
      481 gccacgggag tctacaagct tcgtgtgttg ggaaccgcga aagatgcgct tgggaacgat
      541 atcgagcctt tcctgatgac ctacggattc acggcggaca tcaattgtct cggacaggcg
      601 atcgtgatca gccaggcgta tggaggtggc gggaactccg gtgcgaccta cacgcacgac
      661 ttcatcgagc tacacaatcg gggcgcgatg gacgtcgact tgacgggctg gtccgtgcaa
      721 tttgcttccg cgacgggcac cacttggcag gtcacgccgc ttacgggaac gattgccgct
      781 ggcggttatt tcttgattca gcaagcgaag ggaacgggag ggacgacgtc gcttccggac
      841 ccggacgcga tcggaacgag cgccatgggt gctgagaact tcaagattgc gctcgtgcgc
      901 aattcgacag cattgacggg ggcatgcccg aacgatccgg ccatcgccga ctttgtgggc
      961 gttggcacgg ccaattgttt cgaaggcacg gtcggggcca agctcagcaa caccaccgcc
     1021 gcgcttcgca aggatcaggg atgcacggaa accgatgata atgcagcgga tttcgaagct
     1081 ctagccccca ctccccgcaa caccaagtcg cccatcgtcg cttgctcatg cagtgccaac
     1141 gagacggatg agccttatga aatcgattat tgcgtgttgc aacacccggc atcggtgacg
     1201 gtggtcaaga acacaaccac ggaatccatt tatggccgga tttttgaaac ggggctcacc
     1261 gaggccgcgg gatctgccga ccgggtgttg gcgcaactgg gatgggggcc cacttcggtc
     1321 aatccgagcg cgcagagcgg ttttcaatac gtgaacgcca cgttcaatgt tcaagcgggc
     1381 aacgacgatg aatatgcggc caccatctcg cccgaggtgg cggggaccta ttcgtacgtg
     1441 tatcgattca gcatcgatgg gggcgcgcat tggacgtatt gcgacctgga cggagcgggt
     1501 tcgaacgcaa gtctatcgtt cgacccggcg ctgcttggtg aactgacggt aacggactga
     1561 tcctttcccg atcccttccc accttttttc cgccaatccg ccgcgctgac tggacggcag
     1621 gtatcctttg gtgtcacggt agctcgtcga tgctcatgca aggaggtcac catgctcctc
     1681 gaggttccac gaattcgttc gaaagcaaaa acggcccttg tagccgcggc ggtgcttgcg
     1741 gcgtgcggca gcccatcgga actggatacg attggccaac gagtcagcgc cttatcttcg
     1801 aatctggtca ttgcggaggt ctatggtgga ggggggctca atacgggtgg cacgcctgcg
     1861 ccctactcgc atgacttcgt cgtgctattc aaccgggatt cggcacctgt caatctcaag
     1921 gacttcgtgc tgcaatatgc ctcgcccacc aacaacttca gcaacaagat cgacctaccg
     1981 gatcactccc ttgacgcggg gcaatacttc ctcatccgat tagggacgga taacgctgcg
     2041 gtaggcagcc ccgttcccaa tcccgatttg acaccaacag ccgcgaccaa tatcggagga
     2101 accaatggga aactcgcgct tgcccgtgcg agcaagccct tgagcggctg cgggggttcc
     2161 gcgaatcctt gtcctgccgc ggatatcgtt gaccttctgg gttacggaac ggcatcgcaa
     2221 gcggaaggaa ccgttttgcc ggcgctcaat catacgagtt cggccaagcg caagctggcg
     2281 gggtgtcagg aaaccgatga taataaagaa gatttcgaga ttggggcccc cactccgcga
     2341 ggcacgacaa gtgataaaaa ggattgcgct gccttgcccg atgggggagc ggctggagct
     2401 ggaggcggag gaggatcggg aggttcggcg ggcacagcag gttcggcggg cacagcaggt
     2461 tcggcgggca cagcaggttc agcaggtacc gccggttcgg caggcacagc gggttcggca
     2521 ggcgctggtg atgcgggggt cgtggtaacc gaagggctcg ttcttgccga aatctatccg
     2581 gcgggcggga actcgggtgc cgttttgaaa catgattacg tggtgttgtt caatcgcggc
     2641 acacaatccg tcactctcgg gggtgccgtg ttgcagtatg caagcgccgc gggaaacttc
     2701 gaaaatacgg ctaaatacac catgagcctt ccgaatgagt ccctgcttcc aggcgagtat
     2761 tttctcgtcc agcttgacgg cggctccaac ggtggggacc ttcccacccc tgatgcaagc
     2821 gggacgctcg cgcttggggc aagcgcgggt aaggtagcgc tggccctcgc cactgctccg
     2881 ctgaccgggt gtggctcgag tacaacgcca tgtgattcga aggatatcat cgacttgatt
     2941 ggatacggtt cgacggcatc gcaatacgag ggctctgcaa gcgcgtcggc cccgagcgtc
     3001 accaagtcga tgcaccggaa aaacggtggt tgtgtcgata cgaatgacaa taaaaacgac
     3061 attgacgtgc tggaccccaa cccacgaaac cggtcgacga cgaaatatga ttgttccgcg
     3121 gtggtagatg cgggagcgga tgtggtggcg gatgtggtgg cggatgttca gcaggacgcc
     3181 ccgcaggatg tggttacgga tgtggtggaa gcgggagcag acttcgacgg tgtcgtcatt
     3241 gcggaagtat atggcggcgg cggaaattct ggagcttcgt ttacccacga cttcgtggtc
     3301 ttgttcaatc gaggcaccga gccggtgagc ttggcggggt tgtcgattca gtatgcgtcg
     3361 gcgacgggca gcttttcttc ctcgaacaac acggtagcgc ttcccagcgc cattctcgcg
     3421 cccggcaaat acttcctgat tcaattggct ctcggaacca cgggagatgg aagcgcgttg
     3481 ccaacggcgg acttacaagc gacgatcgcg atgtcggcaa ccgcgggcaa ggtggctttg
     3541 gtgaagtcat cggccctgct ggacaagtgt gg
//
LOCUS       DAOSOR010000590         3568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_26259_length_3568_cov_3.539425, whole genome shotgun sequence.
ACCESSION   DAOSOR010000590 DAOSOR010000000
VERSION     DAOSOR010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26259_length_3568_cov_3.539425"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="PK710_22095"
     CDS             <1..784
                     /locus_tag="PK710_22095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12487.1"
                     /translation="MNHQGWGAPPDDKGATPFAYPEQPVGGFPGAAPGVPFGSEPGMP
                     QPWNVTDSFAFAWNRIKADPGTIIGTMLLGYFISNLPSTIARFIPSDEPSDETSLIVT
                     IVLALLGGLFSTFMMGGMTLFTLNVARGSFYRLNDIFQGGPFFLSILGANLLIGLGVM
                     FGTMLLIVPGIIIALGLCLTTLIIVDRRCGAIEALQQSWRLMNGHKLNVFLWWLMAAV
                     VSLVGILACCIGTIVTAPLLQIAFAFVYLKISGQTTADSVGA"
     gene            complement(891..1967)
                     /locus_tag="PK710_22100"
     CDS             complement(891..1967)
                     /locus_tag="PK710_22100"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HOT12488.1"
                     /translation="MKEVVVVGAGRIGSTIAHMLAHCGDYRITLVDRSQEPLSEIETG
                     PALTTAVLDITDEASLVRLLTGKYAVVSAAPFQLTVAIATVAAKAGVHYLDLTEDVES
                     TRKVIEIAASAKTAFIPQSGLAPGFISIVANDLAGRFDSLDTIRMRVGALPQYPSNAL
                     KYSLTWSTDGVINEYIQPCEAIVDGKRMEVAALEGLEEFSLDGVQYEAFNTSGGLGSL
                     TDTYKGKVQTLNYRTIRYPGHAAIMRMLLQDLRLKDRREVLKDVLEAAVPMTSQDVVV
                     ILVTVLGQRQGRLMQETYANKIYSGLVAGRQMTAIQITTATSACAVFDLLATGKLPQK
                     GFIRQEDITLEAFLENRFGAPYRQ"
     gene            complement(2211..3455)
                     /locus_tag="PK710_22105"
     CDS             complement(2211..3455)
                     /locus_tag="PK710_22105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12489.1"
                     /translation="MRLGVFSFMVCGGGCVASTVFAGDWEHSDDFELGYQGSYVFEAR
                     TLSRNRPYASAAHRTSVYSEESPTRWRLDASLRGWVDVRGDLENEVQPELDVRELSLS
                     RADDYTDIRLGLQQIAWGETFGVPIADVVNPRDLRDPLFLEMDWVRRPTWTANVQVMV
                     ESLRLQAVATPLPRNNELPERGSAYDPFPPVLKGVEVEPQREFSLGRFGRDGEYGGRL
                     GYLFDFGLDVGLLYFFHWNRVPVYELAMQGGRPVVVPFQDRIHTAGITVSMAFEDWVL
                     RGDGVMHGNVPWMTRDLQGTRRIDYLQAIVGVDRTTESGWTLGAQFHIDYRGDRMLTW
                     GSGRIQKSLWDGDLEPQIFAYIGVDNTDRWVQPRIDWHVLSLWTLSVRADLVWGTTGD
                     RAGDLGFIDGRHRVMMWTSLRL"
BASE COUNT          779 a         1199 c          852 g          738 t
ORIGIN      
        1 tatgaatcat caaggatggg gggctccccc cgacgacaag ggagcaacac ctttcgcgta
       61 tccagagcaa ccggtaggcg gatttccagg tgctgcccct ggtgttccgt ttggctccga
      121 acctggaatg ccacaacctt ggaacgtcac cgactccttc gctttcgcct ggaatcggat
      181 caaagccgac cccggcacga tcatcggcac gatgctgctc ggctacttca tctccaatct
      241 cccttcgacc atagccaggt tcatcccgtc cgacgaacct agcgacgaaa cctctttgat
      301 agtcactatc gtgctcgccc tcctcggtgg tttgttttcc acgttcatga tgggaggcat
      361 gacgctattt accctcaacg tagcgcgtgg aagcttctat cggttgaacg atattttcca
      421 aggcgggccg ttttttttga gcatcctcgg cgccaacctg ctcatcggcc tcggcgtgat
      481 gttcggaact atgctgttga ttgtcccagg catcatcatc gctctcggat tgtgtctgac
      541 gactctcatc atcgtcgatc gtcgatgcgg cgccatcgag gccctccaac agagctggcg
      601 gctcatgaac gggcataagt tgaacgtttt cctgtggtgg ttgatggctg ccgtcgtatc
      661 tttggtcggt attctcgcct gctgcattgg cacgatcgtc acggccccct tgctccagat
      721 cgctttcgcc ttcgtgtacc tcaagatttc aggacaaacc acggctgact ctgtcggggc
      781 ctgaccccgg atctcgaccg agggcaggtc gagcgggcac caagacagct tgtgattact
      841 agttgtgcgc tgcttgcatg gcgtgggaag cgcaggggga aagcaacgat ctactgacga
      901 taaggcgcgc cgaagcgatt ttccaaaaat gcttccagcg tgatgtcctc ctggcgaatg
      961 aaacccttct gcggcagctt gccggtagcc aacaaatcaa ataccgcaca cgcactcgtc
     1021 gccgtcgtaa tctgaatcgc cgtcatctgt cgacccgcga ccagcccact gtaaatctta
     1081 ttggcgtacg tttcttgcat caatcggcct tgacgctgac ccaatacggt cacgagtatc
     1141 accaccacgt cctgggatgt catcggtacc gcggcttcca gcacgtcctt gagcacctca
     1201 cgacgatcct tgagccgcaa gtcttgcaac aacattcgca tgatcgccgc atgacccgga
     1261 taacgaatgg ttcggtagtt cagcgtttgc acctttccct tgtaggtatc ggtgagggaa
     1321 cccaacccgc ccgacgtgtt gaaagcttca tattgcacgc cgtccagcga gaactcctcc
     1381 aagccttcga gggcggcaac ttccatacgc ttgccatcca cgatcgcttc gcacggttga
     1441 atgtattcgt tgatgacccc atccgtgctc caggtcagac tgtacttcaa cgcgttgctc
     1501 gggtactgcg gcaacgcccc aacgcgcatg cggatcgtat cgagagaatc gaaacgcccc
     1561 gccagatcat tggccacgat ggaaatgaac cctggcgcaa gaccgctttg cggaatgaac
     1621 gccgtcttcg cggatgccgc aatctcgatc accttgcgcg tcgactccac atcctccgtc
     1681 aagtcgagat aatgaacccc cgccttggcc gccaccgtcg ctatcgccac cgtaagctga
     1741 aacggagcgg cactcacgac agcgtacttc ccagtgagaa gacgaacaag cgacgcttca
     1801 tccgtgatgt ccaacaccgc ggtcgtcagc gctggccccg tttcaatctc ggaaagcggc
     1861 tcctggctgc gatccacgag cgtaattcga tagtccccac aatgcgcgag catatgcgcg
     1921 atggtagagc cgatacgacc tgctccgaca acgacgacct ctttcatggc gttctccctt
     1981 aagcgatagg ttcgcgttcg attgttgcag cctgcctttc gaatcgccga ggcgtttcct
     2041 attcctgcgc tggatacctc cgccacaggc agagtacttc cgcatcgaat cgatgccgcg
     2101 caaggacgcg ctgcgtccgt tctggtaatg gacatcgagc ggttgtgcaa gcctttccac
     2161 caccgaatcg acgaactccc cgctgcctgc ttcccccccc cagcctcccc tcacagccgt
     2221 aaactcgtcc acatcatgac gcgatgacgc ccatcgatga accccagatc tccagcacga
     2281 tcacccgtcg tcccccacac cagatccgcg cgcacggaaa gcgtccataa cgacaacaca
     2341 tgccagtcaa tccgcggctg cacccatcga tccgtattgt ccaccccaat atacgcaaaa
     2401 atctgtggct ccaaatcgcc atcccatagc gatttctgaa tccgtccgct gccccacgtg
     2461 agcatcctat cccctcgata atcaatgtga aattgcgctc ccagggtcca tccggattcc
     2521 gtcgttctgt ctacccccac aatcgcctgg agatagtcga tacgccgcgt cccttgcaga
     2581 tcccgcgtca tccacggcac attcccatgc atcaccccat cgcctcgcaa cacccaatcc
     2641 tcgaacgcca tggaaaccgt tatccctgcc gtgtgaatcc gatcctgaaa cggcaccacc
     2701 acgggccgcc ccccctgcat cgccagctcg tacaccggaa cacgattcca atggaaaaag
     2761 tacaatagcc ccacatcgag gccaaaatcg aataaatacc ccaatcgtcc cccatattcc
     2821 ccatcacgac cgaatcgacc aagcgaaaac tcacgctgcg gctccacctc gacgccctta
     2881 agaaccggcg gaaaagggtc gtaggcagaa cctcgctccg gcaactcgtt gtttctcggc
     2941 agtggcgtcg ccacggcttg caaccgcaac gactccacca tgacctgcac attggctgtc
     3001 caggtcggac gacgcaccca atccatttcc agaaacagag ggtcgcgcaa atcccttggg
     3061 ttgacgacat ccgcgatggg caccccaaag gtttctcccc acgcgatctg ttgaagcccg
     3121 agccgaatat ccgtgtagtc atcggcgcga gaaaggctca gctcccgcac atcgagttcg
     3181 ggctgcacct cgttttccag gtctccccga acatccaccc agcctcgcag cgatgcatcc
     3241 aaacgccaac gggtcgggct ctcctcggaa tacaccgaag tccgatgcgc tgccgaagca
     3301 tagggacggt ttcgcgatag cgtcctggcc tcgaatacat aagacccttg ataccccaat
     3361 tcgaaatcgt cggaatgctc ccaatccccc gcgaaaaccg tcgaagccac acacccccca
     3421 ccgcatacca tgaacgaaaa tacaccaaga cgcaccaccc ccttgtatca cgatagggtg
     3481 atttcgtcgc cccaagccgt ccggctactc gagattctcg ggcacgaaca atgaggccgg
     3541 gaacttgcgc acctccatgg ccaacacc
//
LOCUS       DAOSOR010000591         3566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_26281_length_3566_cov_4.154942, whole genome shotgun sequence.
ACCESSION   DAOSOR010000591 DAOSOR010000000
VERSION     DAOSOR010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26281_length_3566_cov_4.154942"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="PK710_22110"
     CDS             <1..1334
                     /locus_tag="PK710_22110"
                     /inference="COORDINATES: protein motif:HMM:NF013560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HOT12490.1"
                     /translation="LLEKMVNASSEIEKTFNTFRAQLEGKKYSNNELLDMLAKQKDSK
                     KRQAIWEGLKQVGERVGPQIIALAKIRNEAATKLGYANYWDMQVRLQEHDPERLMTLF
                     QELENLTNAPFAAMKTKLDEELAQKLHVRPEEMMPWHYDNPFFQDAPPSSNVDLDDFY
                     QGKKKEDIVAIAKQFYSDIGIDITPVLDKSDLYERDGKDQHAFCIAIDREGDVRTLLN
                     IKPKAEWMDTMLHEQGHAVYYRLLDHELPYNLREAAHIFTTEAIAMMFGALAKNPTWM
                     THYVGADKKKVKAAEADILEQRRREQLIFTRWTLVMLHFEKALYENPDQDLNQLWWDI
                     VEKYQSLRRPPHRHAADWASKPHFTIAPVYYHNYMLGELFASQLRASLATFARHEGPT
                     STLSFNGRKEFGEFLTNKVFKPGMRMKWPQFVRAATGEELTAKYFAREVAR"
     gene            1411..2814
                     /locus_tag="PK710_22115"
     CDS             1411..2814
                     /locus_tag="PK710_22115"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOT12491.1"
                     /translation="MRAFKTPVSTFVARRNALHATIRTPALLAAGALRARNYPANGYP
                     FRASSHFLYLVGAALPSAVLWLSDGKSTLFIEPEEPGADLWDGPKPSFDSLRETLGMD
                     QVRDLSELESAIEAQRNSIATLPTQDAASCELLSRCLGRQIQPGSGASLKDGAPDAAL
                     ADAMIALRLRHDDAALEQLRWAASVTAQAHLAGMRATRTARSETEIFGAMMGVLRTAG
                     HEDSFGPIVSKNGEVLHNIRHDNALADGDLVLADVGGETPEGWAGDITRVWPVSGTFS
                     PTQRAIYDVVLAAQCKAIERVRIGVRYRDIHETAKRVMVQGVRDLGIFRGEVDGLLER
                     GAAAIFFPHGIGHLLGLDVHDMEDLGDRAGYAAGRTRSKAFGDCYLRLDRDLEPRMAV
                     TIEPGFYQVPAILASPAYTAAVGNDLRRDVLAKYADVRGIRIEDDVVVTQGDPEVLTK
                     EVPKTAEEVEAIVQSGR"
BASE COUNT          845 a         1055 c          974 g          692 t
ORIGIN      
        1 aactgctcga aaaaatggtg aatgccagca gcgaaatcga aaaaactttc aacacctttc
       61 gcgcacaact cgagggcaaa aaatactcga acaatgaatt gctcgacatg cttgccaagc
      121 agaaagattc gaaaaaacgc caagccattt gggagggact caaacaagtc ggcgaacgag
      181 tcggacccca gatcattgcc cttgccaaaa tccgaaacga agccgctacg aaactcggct
      241 acgccaacta ctgggacatg caagtgcgcc tgcaagaaca cgaccccgaa cgtctgatga
      301 ccctctttca agaactagaa aatctcacca acgccccctt tgccgccatg aaaaccaagc
      361 tcgatgaaga gctggcccaa aagctccatg tccgtcccga ggaaatgatg ccttggcatt
      421 acgataaccc gttttttcaa gacgcccccc cctcgtcgaa cgtggacctc gatgacttct
      481 accaaggcaa aaaaaaggaa gatatcgtcg ccatcgccaa gcagttctac agcgatatcg
      541 gtatcgatat cactcccgtt ctcgacaaaa gtgatttgta cgaacgagac ggcaaagacc
      601 aacatgcgtt ttgtatcgcc atcgatcgcg agggagacgt tcgcacctta ttgaatatca
      661 agccgaaagc cgaatggatg gatacgatgc ttcacgagca aggacacgcc gtgtactacc
      721 ggctgctcga ccacgaactc ccttacaact tgagagaagc ggcccatatt ttcaccaccg
      781 aggcgatcgc catgatgttc ggcgctctcg ccaaaaaccc cacgtggatg acccactacg
      841 tgggagcgga caaaaagaaa gtcaaagccg cggaagcaga tatcctcgaa cagcgcagac
      901 gcgagcagct catcttcaca cgctggaccc tggtgatgct gcacttcgaa aaagccttgt
      961 acgaaaaccc cgaccaagac ctcaaccaac tctggtggga tatcgtcgaa aagtaccagt
     1021 cgttgcgtcg ccccccccac cgtcacgccg cggattgggc ctcgaaaccg catttcacca
     1081 tcgctcccgt ctattaccac aactacatgc tcggagaact tttcgcgtcg cagttgcgag
     1141 cctcgctcgc caccttcgca cgacacgaag gcccaacctc caccctttcg ttcaatggtc
     1201 gcaaggagtt cggtgagttt ttgacgaaca aagtcttcaa acccggcatg cggatgaaat
     1261 ggccccaatt cgttcgagcg gccacgggtg aagaactgac ggccaagtat ttcgctcgtg
     1321 aggtggccag gtaacacgat ccgccagctt cccgttgctc ttccactctt tcgttgtgtc
     1381 tcacccgcca ggctcattac actggcgctc atgcgcgctt tcaagacccc cgtctccacg
     1441 ttcgtggcac gtcgaaacgc tttgcatgca acgatccgca cgccagcctt gcttgccgcc
     1501 ggtgcgcttc gggcccgcaa ctatcccgcg aacggctatc cgtttcgagc cagcagccac
     1561 ttcctctatc tggtgggagc cgccctaccg tccgccgtac tatggctaag tgatgggaaa
     1621 tcaacgcttt ttatcgaacc cgaggaacca ggggccgacc tttgggatgg ccccaaacca
     1681 tcgttcgact ccttacgaga aacacttgga atggaccagg tgcgggacct gtccgaactc
     1741 gagtccgcca tcgaagcaca acgcaactcg atcgcgactc tccccacgca agacgccgct
     1801 tcctgtgagc ttctttcccg gtgcctggga cgacaaatac aaccaggttc cggtgccagc
     1861 ctaaaggatg gagcgccgga cgctgccttg gcggatgcga tgatcgcgtt gcggttgcgt
     1921 catgacgatg cggcactcga acagcttcgg tgggccgcca gcgtcacggc gcaagctcac
     1981 ttggcgggta tgcgcgccac ccggacggct cgctcggaaa cggaaatttt cggcgcgatg
     2041 atgggcgtat tgcgcaccgc gggccacgaa gactcgtttg gccccatcgt gagcaaaaac
     2101 ggcgaggtgc tgcacaatat ccgccatgac aatgccttgg cggacgggga cctggttctc
     2161 gccgatgtcg gaggggagac tcccgaaggg tgggcggggg atatcacgcg ggtatggccc
     2221 gtgtccggaa cgttttctcc cacccagcgg gcgatttacg atgtcgttct cgcggcacaa
     2281 tgcaaagcca tcgagcgagt tcgcataggg gttcggtatc gtgacattca tgagacggcc
     2341 aaacgcgtca tggtgcaggg ggtccgggac cttgggattt ttcgcggcga ggtcgatggt
     2401 ctgctcgaac ggggtgccgc ggcgatcttc tttccccatg gaatcggcca cctgttaggg
     2461 ctcgacgtcc acgacatgga agacctgggg gatcgcgcgg gatatgctgc gggaagaacg
     2521 cgttccaaag cctttggaga ctgctacctg cggctcgacc gggatctcga accgcgtatg
     2581 gctgtgacca tcgagcctgg tttctatcaa gtgccagcca tcctggcgag tcccgcgtac
     2641 accgccgccg taggaaacga cttgcgtcgc gatgtgttgg ccaagtacgc cgacgttcgg
     2701 ggaatacgca tcgaagacga cgtcgtcgtc acgcaaggcg atccggaggt tctgaccaag
     2761 gaagttccga aaacagccga agaagtggag gccatcgttc agtcgggccg ttgacggcaa
     2821 atccacaacc ccgcggatcc gaccggtggt tgacaggtat ccatactcgg acaccttttc
     2881 atccatccgt ggtgccgtac aaaccgcctg cccgatggag atagccaatg cgacggtcga
     2941 ggcggcacca gctcatttca atccacgatg cacggcatgg ctcgacggcc aacgagaacc
     3001 aatttgctgc cgtgggagat gcatcgaagc ggctggcaga gagagcccca actgtcgttt
     3061 ccacgcttcc gaacgggtga cggatgtgga tggttcagac ggtctcctcg gtaacgacgg
     3121 aaacagcgaa ttgactttcg cgcacgtatc caaagacctc agactgttgt tagaaaacgt
     3181 tcactacatc attatcagac aaacagacgg aacgagcgtc ggcctggggg tatcggcatc
     3241 atcgggaacg acctgcgccg cggtagaatg gcaacgcact ccgatcttcg gaaaactcgc
     3301 atcgaagacc aaaccatcgt cgagccaagg ggatcgaggg ttctgcccaa ggaggttccg
     3361 aaagcagcca cgatggtgga ggttgtcatg caggggggct gtcgatgaca agctggacca
     3421 acacgaaaca ggccgttgtt taccgcccat ccaggcttgg gtatcctacc ataccagcgg
     3481 tattgctctg tctccatttt caattgcata gaggctactc aatgaacatg aagcgaatgc
     3541 tgggaattgg cttttcggtg gggctg
//
LOCUS       DAOSOR010000592         3565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_26290_length_3565_cov_3.945299, whole genome shotgun sequence.
ACCESSION   DAOSOR010000592 DAOSOR010000000
VERSION     DAOSOR010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26290_length_3565_cov_3.945299"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PK710_22120"
     CDS             complement(<1..364)
                     /locus_tag="PK710_22120"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOT12492.1"
                     /translation="MSNEPVFVGLDCSTHGAKAIAWSLQGEPKAQASSSFPLHNPHAG
                     FWEQDPRDWLEASQRVLSQIARELGPRVAAIAITHQRETIVGVDDAGSPVRPGIVWMD
                     ERSRDDVQHLRQVVGADSF"
     gene            complement(463..1428)
                     /locus_tag="PK710_22125"
     CDS             complement(463..1428)
                     /locus_tag="PK710_22125"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HOT12493.1"
                     /translation="MKTSTAVFQYLNATCRGSGQVVFMNNPITGLLNVAALLWAAYFG
                     GTTIFVALGAILGTFVSTITAYPLHADRESIRSGLYGFNGMLVGAALPTFLDNTPSMW
                     ALLLLATITVTFVTIALERLLSTWKIPGLTFPFVLTSWIVLLATYRFTAAAPAGLPKA
                     QLIAPAVAVDHVWTLGDFLHASLTSVSQVFFVNNPVSGAIFLFALAVHSRWCALLAAS
                     GAALAVVTAWLLGADPTLILHGLWGYSAALTAPAIGCIFLKPSARSLALSALAVGFTV
                     LVQAATASVSETVGLPALTFPFVLTTWIFLLAKPGVESESAEPNA"
     gene            1843..3138
                     /locus_tag="PK710_22130"
     CDS             1843..3138
                     /locus_tag="PK710_22130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12494.1"
                     /translation="MRASAEQEHEARVPVVGTPKSATGRERETFESDESGRTVADVAL
                     YLPRNVLDLLFITTGAASGLIEDEQVVPRLKDTFYSENHEIGVFPTAFVETGFTPNIG
                     ARMIASIDNFASTLRAGYGGPDSTVVESRIRMTSGCRIPLSLSIEWLQDRRTDLVFMG
                     LGPSPEQDPRNQFRPLQLSRNGLYRERRQRMIAGVGVRLAPDWVLVLSNGLKSRHVDD
                     APDAGSDQLSRVFEPSSIPGANRITRTVYTEAALRFDSRENRGAPTTGAMMEAYGGVY
                     NGIRSQGASAVASGVRWAAFLPLYRVTNVISPRITIDSITPLGDAPLPFFDYLSAADF
                     RGFDGRRDRVVMVASVDYRWTLIQYIAARVFGDATNVAPEVSSLALEHARYAWGLGFD
                     LHSASTQIGRVAASFSQGDMRLFLSLGVAPSGFGDRQHR"
     gene            3331..>3565
                     /locus_tag="PK710_22135"
     CDS             3331..>3565
                     /locus_tag="PK710_22135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12495.1"
                     /translation="MPSRWLAIAVIVLGCLSATSCVHRPARFADRPIVTDIADDAPID
                     LPRRIELNWDWYLSDAFLRRSLFDALNPNRPDDA"
BASE COUNT          782 a          973 c         1051 g          759 t
ORIGIN      
        1 gaaaggaatc agcgcccaca acctgccgaa ggtgttggac atcgtcgcgg gaacgctcat
       61 ccatccaaac gatgccaggg cgaacgggac ttcccgcatc gtcgacaccg acaatggttt
      121 cccgttgatg ggtgatggca atcgcagcca ctcgtggccc aagctcgcga gctatttggg
      181 acaatacccg ctgagacgcc tcgagccaat cgcgtgggtc ttgttcccaa aaacccgcgt
      241 gaggattgtg caaaggaaaa gaagacgatg cttgggcttt gggctctcct tgcagggacc
      301 acgcgatcgc tttggcacca tgggtcgagc aatcaaggcc cacgaacacc ggttcgttcg
      361 acacgacgca agcatcgaat gaaccagcga gaaggtcaac cgcatccgtg tgcgacgata
      421 atcattggcg gtatcgtccg catcctgcag gtcgacaagg gctcaagcgt ttggctcggc
      481 cgattcagat tcgacaccag gtttcgccaa gagaaagatc catgtcgtca agacgaaagg
      541 aaacgtcagg gctggtagac ctaccgtttc cgaaacgctg gcggtagctg cttgaacgag
      601 tacggtaaag cctacggcga gtgcgctcaa ggctagtgat cgcgcgctgg gtttcaaaaa
      661 aatacatccg atggccggtg cggtgagtgc cgcggaatag ccccacagac catggaggat
      721 gagggttggg tcggcgccaa ggagccaagc ggtgacgacg gcaagtgcag cgcctgaggc
      781 ggcaagcagc gcacaccaac gagaatgcac cgcaagagcg aacaaaaaaa tcgctcctga
      841 aacaggattg ttgacgaaaa atacttgaga cacactcgtc aacgaagcgt gcagaaaatc
      901 cccaagggtc cagacatgat cgacggccac tgccggagct atcaattgtg ccttgggcaa
      961 ccctgctggg gcggcggccg taaaccgata ggtggcaaga agaacgatcc aactggtaag
     1021 cacgaaaggg aatgtcaaac ccggtatttt ccatgtgctc agcaggcgtt cgagagcgat
     1081 ggtgacgaag gtgacggtga tggtggcaag cagcaaaaga gcccacatcg acggagtgtt
     1141 gtcgagaaac gtgggcaagg ctgctccgac gagcatcccg ttgaatccat aaagacctga
     1201 acgaatggat tcgcgatccg cgtgcaaggg ataggcggtg atggtggata cgaaagtgcc
     1261 aaggatggct cccagggcca cgaagatcgt ggttccacca aaataggcag cccagagcaa
     1321 ggcggcgaca ttgagcaacc ctgtgatggg gttgttcatg aaaacgactt gtccagaacc
     1381 ccggcaggtg gcgttcaggt attgaaagac cgctgtggat gtcttcatgg gaaggtcgat
     1441 aggagcgagc cgtgagggcg gggagcaccg aaagggtccg ccccgctccg tcatcaaaag
     1501 cgtaacgatg gtcgaccagg gctctggaaa cgggtcgccg tggcagatac ctagggagta
     1561 aaccccggat acgtcaagat tgaccctgct gtgcctcggg ttttgggtgg gctcatttcc
     1621 tagcgtgaag gtgaatccgg gtcgtacggg atgaaatcaa gcatattcat caggacattg
     1681 acggtttgtg gagacaagct gcattggctg ttgtcacggt gcagcttttc ttgaaggatt
     1741 cttctggcgt ctatctttgc tggttcccat cgtgcgcagc ttatggccct tcgtcgcttc
     1801 cggtcttcct gttctatcct aatatggata ctatgcattt cgatgcgggc cagcgccgag
     1861 caggagcatg aagcacgggt tcccgtggtg ggtaccccga agagcgcgac ggggcgtgag
     1921 cgagaaacgt tcgaaagcga cgagtccggg cgaacggtgg cggatgtggc cctgtacttg
     1981 ccacgcaacg tgctggatct tctgttcatc acgactggtg cagcgagcgg gctgatcgaa
     2041 gacgagcaag tggtccctcg gctgaaagac acgttttaca gcgagaacca tgaaattggt
     2101 gtgtttccaa cggcttttgt ggaaactggg ttcacgccga acatcggcgc ccgcatgatc
     2161 gcaagcatcg ataacttcgc atccaccctg cgggccggat acggaggacc ggattcgacg
     2221 gtcgtggagt ctcggattcg gatgacttcg ggctgtcgta tcccgttgtc cctttccatc
     2281 gaatggcttc aggaccgacg caccgacttg gtattcatgg ggctcggccc ttctccagag
     2341 caggaccctc gtaaccaatt ccgaccgttg caattatccc gaaatggatt gtatcgcgag
     2401 cgtcgtcaac gtatgattgc cggagttggc gtgcgcttgg cccccgattg ggtactcgtg
     2461 ctgtccaacg ggcttaagtc gcgtcatgtg gatgatgcgc cagacgccgg cagtgaccaa
     2521 ctgtccaggg tattcgagcc atcttccatc cctggtgcca acaggattac gcgaaccgtc
     2581 tacacggagg cggcgttacg gttcgactct cgcgagaatc gaggcgctcc aaccaccggt
     2641 gccatgatgg aagcctatgg tggcgtgtac aacggcatac ggtcacaagg cgccagcgcc
     2701 gtggcgtccg gtgtgcgttg ggccgccttc cttcctcttt atcgggtcac caatgtcatc
     2761 tcccccagaa tcaccatcga ttccatcaca cccctggggg atgccccgtt accgtttttc
     2821 gactacctta gcgcagccga ctttcgagga ttcgatggcc gacgcgatcg cgttgtgatg
     2881 gtggcttccg tcgattatcg atggacgctc atccaatata tcgcggctcg tgttttcggc
     2941 gatgccacca atgtggcccc ggaggtctca tcgcttgctc tcgaacacgc tcgttatgcg
     3001 tggggccttg gtttcgatct gcatagcgcc agcacccaaa tcggtcgtgt cgcggcttct
     3061 ttcagccagg gagatatgcg gctttttcta tcccttggag tggccccctc cggcttcggc
     3121 gaccgtcaac accgctaatt cataactata tggttttatt gatattatat gaaatcgaaa
     3181 cctgctcgat ccctccgttc gtctccctgt cgaagcatca tgaaatggca agacgttcct
     3241 tttcgcaagg acccaaggct aagatcgaaa agtctcgtgg ttgatggtca cgatccattg
     3301 tgtccgggct ccagattgcc tggggatgtc atgccctcgc gatggcttgc catcgccgtg
     3361 attgtccttg gctgcctttc ggcgacgtca tgcgtacacc ggcccgcgcg gtttgccgat
     3421 cgccccatcg ttaccgacat cgccgatgat gcccctatcg acctgccaag acgcatcgaa
     3481 ttgaattggg attggtacct atcggatgcc tttttgcgaa gatcgctttt cgatgcgctc
     3541 aatcccaacc gtcccgacga cgcgg
//
LOCUS       DAOSOR010000593         3563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_26307_length_3563_cov_3.859464, whole genome shotgun sequence.
ACCESSION   DAOSOR010000593 DAOSOR010000000
VERSION     DAOSOR010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26307_length_3563_cov_3.859464"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="PK710_22140"
     CDS             <1..860
                     /locus_tag="PK710_22140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12496.1"
                     /translation="LLHLGDQGVIESSRWFSNQQFLYPLQLVAVDDGVVVAGMDWDKK
                     SNYRGLVLARIRLDGTVVWAKRFGSCGGSGYPGMQPTDARRLVNGDILVTGLLGLGQR
                     SFMMEVKPEGQVTWATALSADDPLSELAIHSVRELPTTGFLAAGRYHHHYDPYRVFLA
                     GLDAKGRTLWLKSYGQPSQESGLPAKQVFPSIQLTDDGAALLAAYSTAPIPSENRNVW
                     MFKAAAVDGAVAFRPGEAEVLEFPHASVECDLTPEAVSLTVSDLDLAPASFVPSIENR
                     NISVESLDP"
     gene            1030..1674
                     /locus_tag="PK710_22145"
     CDS             1030..1674
                     /locus_tag="PK710_22145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12497.1"
                     /translation="MMNTRWLGILCVGIGFWACSGTVETASSSGGGSAGAGGTPAGGS
                     GGASIGGTAGSAGTEPMAGGAGEAAAGGDAAAGGDAAAGGDATACKPQDVQAQGPCAM
                     VLGMYWDGAACVTLSGCSCVGSDCSHGFEESHAFPTGILSAQETCEKAYRGCLTCAPQ
                     DGHLAGNCAFLGPIIRWDGKKCVQETMGCSCKGPDCPSLYSNSGECIKAHAACP"
     gene            complement(1784..2215)
                     /locus_tag="PK710_22150"
     CDS             complement(1784..2215)
                     /locus_tag="PK710_22150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12498.1"
                     /translation="MVQPNNLRTSAGGLKPWPQINRRTHFKKPAMSIAVRKRGGNRGV
                     RLPGVWHSHSHQLGRKVLASFDIDSIQCPCHTLQMGGYGPNFNPKNAPNGIQGSARPE
                     AKRYSGVGLQSLQNKFFGPLHGITCHRFDVGGSRTTLSARC"
     gene            2338..>3563
                     /locus_tag="PK710_22155"
     CDS             2338..>3563
                     /locus_tag="PK710_22155"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HOT12499.1"
                     /translation="MRISSTAGVAFTLFGLGACIAIPEGDGIPSRITDRDDASADAFI
                     NVEANAPLQDAGIDRPITDPHAILGVDPSHGPFSGGRVAVIRGNGFKSTARVWFGQVE
                     VPSSDVTAGDPMRIQVLVPPGDPGQVDVRTQNGDDASTSRTLQSAYTYDAFYAEPSKG
                     PTSGGTIVKLIGKSTNWGSTAEVRVSNEPCLTSKIVSPSEIECVTPPQPAGSVAISVK
                     MPGEATQVVYDAFTYSDSDNGYKGGLSGGKMQGVLKVLVYDAYTGSPIPGARIIVGDN
                     LDSAFLGTTDQAGLHVFSEPGLSDKRSVTIAKSCFQPVTFVDVPVDTVTAYLPPVLSP
                     ACLGDGGEIPPVGGKGATLATIKGQLVWYGGTEFKRAHWSNVPSPKGPDERQAAYLFQ
                     PSSDPTSKFYLPDASRA"
BASE COUNT          701 a          910 c         1170 g          782 t
ORIGIN      
        1 ggctgcttca tcttggcgat caaggcgtta tcgaatcatc ccgttggttt tcgaaccagc
       61 agtttttgta tccgctacag cttgtggcgg tggacgacgg ggttgtggtc gcggggatgg
      121 actgggataa aaaatccaat tatcgcgggt tagtcctggc gcgcatccgg ctcgatggaa
      181 cggttgtatg ggcaaagcgt ttcggctcgt gtgggggcag cggatatcca gggatgcagc
      241 ccacggatgc ccggcggctc gtcaacggtg acatcctcgt gacaggcctg ctgggccttg
      301 gacagagaag cttcatgatg gaagtgaagc ccgaagggca ggtgacctgg gcaacggcct
      361 tgtcggctga tgacccgcta tcagaattgg cgattcattc tgttcgagag cttcccacca
      421 caggattcct ggcggcgggg cggtaccatc accactacga tccttaccgc gtatttcttg
      481 cgggcctcga tgcaaaaggg cgcacgcttt ggctcaaaag ttacgggcag cccagtcaag
      541 aaagcggact cccggcaaag caggtttttc cttcgattca gttgacggat gatggggccg
      601 cgctcctggc agcctattcg acagcgccca tccccagcga gaatcgtaac gtctggatgt
      661 tcaaggccgc ggcggtcgat ggcgcggtgg cttttcgacc gggcgaagcg gaagtgctcg
      721 agtttcccca tgcttctgtc gaatgcgatc tgaccccgga agccgtttcc ctgacggtat
      781 ccgacctcga tcttgccccg gcatccttcg ttcctagcat cgaaaaccgc aatatctccg
      841 tcgaatcgct cgatccatga gggccgaggc tcgtacgcac ggccgtcccg cagacaataa
      901 tttacaataa tatcaacaac ttatatcaca gcctgcgtat catgggaaga tcggatacgg
      961 tgggtagggt gtcgggaaag aatcaggatg gtaagtgcgc gtatgggaag aaaaggatat
     1021 tctacgttca tgatgaatac tcgatggctg gggattcttt gcgtggggat tggtttttgg
     1081 gcctgcagtg gaacggtgga gacggccagt tcgtcgggtg ggggaagtgc cggagctggg
     1141 ggcacgccgg cgggtggatc ggggggtgcg tcgattggcg gcacagcggg ctcggcagga
     1201 acagagccga tggcaggcgg cgcaggagag gcggcggcag gaggtgatgc ggcggcagga
     1261 ggtgatgcgg cggcaggagg tgatgcgact gcctgtaagc cgcaagacgt tcaagcccaa
     1321 ggtccttgcg cgatggtatt gggcatgtat tgggatgggg cagcttgtgt caccttatcg
     1381 gggtgctcgt gcgttggttc tgattgctcc catgggttcg aagagagtca tgcgtttccc
     1441 acggggatac tgtccgcgca ggagacgtgc gaaaaagcgt atcggggttg tctgacctgc
     1501 gctccccagg atggacatct cgcgggcaat tgcgcatttt tgggcccgat tatccgatgg
     1561 gacggcaaaa aatgtgtcca ggaaacaatg ggatgcagtt gtaaaggacc ggattgcccg
     1621 agtctgtatt cgaattcggg ggagtgtatc aaagcgcacg ccgcttgtcc gtagctacgc
     1681 ttgtcctacg ccggccatcc aattcgacgg cactatgacc aggcacgtag ggtgttcagg
     1741 ttgctgtctt ggaggccggg aagatggcca caaagtatac tgctcaacag cgcgccgaaa
     1801 gcgttgtgcg cgagcctccg acatcgaagc ggtggcaagt gatgccgtgc agcggaccga
     1861 agaatttgtt ttgcaagctt tgcaagccga ctccactata gcgtttcgct tctggtcgtg
     1921 ccgagccttg gatgccgttg ggtgcgttct ttgggttgaa gttagggccg tatccgccca
     1981 tttgcagcgt atgacagggg cattgtatcg agtcgatatc aaacgatgcg agcactttcc
     2041 ggccaagctg atggctgtgg ctgtgccaga cgccgggtaa tcggacaccg cgattgccac
     2101 cacgcttgcg cacggcaatg ctcattgccg gttttttaaa atgggtacgg cgattgatct
     2161 gcggccatgg cttcaggccg cctgccgatg tccggagatt gtttggttgg accatgcttg
     2221 acaagcgccg taaagaccgt gatgcgcttc aggcctgtcg tctggcaacg gctccggaat
     2281 tcggaggaat ccactcgagt accgcccgcc acgaagctgt actatgcttg ctggaccgtg
     2341 cgtatctctt caactgctgg cgtggctttc acccttttcg ggctcggggc ttgtattgcg
     2401 attcccgaag gcgatgggat tccgtcgcgt atcactgacc gtgatgatgc gtccgccgac
     2461 gccttcatca acgtggaagc caacgcgcct ttgcaagatg ccggaatcga tcgcccgatc
     2521 accgatcccc atgcgatcct tggcgtcgac ccatcgcatg gcccgttttc cggaggtcgc
     2581 gtcgcggtga ttcgaggaaa cgggttcaag tcgacggcaa gggtatggtt tgggcaggtg
     2641 gaagtgccgt cgtcggatgt gacggcgggc gatccgatgc gcattcaggt cctggttccc
     2701 ccaggcgatc cggggcaagt agatgtacgc acccaaaatg gcgacgatgc atccacgtcg
     2761 cgaaccctgc aaagcgcgta tacctacgat gctttttatg ctgaaccttc gaagggaccg
     2821 accagcggtg ggacgatcgt caagctcatc ggcaagtcta cgaattgggg gtcgacggcg
     2881 gaggtgcgtg tatccaacga gccgtgtttg acgagcaaga tcgtgtcacc cagcgagatt
     2941 gagtgtgtga cgccgcccca gcccgcgggc agtgtggcca tttcggtcaa gatgccgggg
     3001 gaagcaacgc aggtggtgta cgatgcgttt acgtattccg acagtgacaa cgggtacaag
     3061 ggggggcttt cgggcggaaa gatgcagggc gtgctgaagg tcttggtgta tgacgcctat
     3121 acgggcagtc cgataccggg ggcgcgtatc atcgtgggag acaatcttga ttcggcgttc
     3181 ctcggcacca cggatcaggc ggggcttcat gtgtttagcg agccagggct ttcagacaaa
     3241 cggtcggtga cgattgccaa gtcttgtttt caaccagtca cgttcgtgga tgttccggta
     3301 gatacggtga cggcgtattt gcctccggtg ctgagtccgg cttgtcttgg ggacggtgga
     3361 gagattccgc cggtgggagg aaagggagca acgctagcca ccataaaagg gcagctcgta
     3421 tggtatgggg ggacggaatt caagcgagcg cattggtcca acgttccgtc accgaagggg
     3481 ccggacgagc ggcaggctgc ctatttgttc cagccaagtt cggatccgac ctcgaagttt
     3541 tatcttcccg atgcatcgcg ggc
//
LOCUS       DAOSOR010000594         3561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_26320_length_3561_cov_4.309469, whole genome shotgun sequence.
ACCESSION   DAOSOR010000594 DAOSOR010000000
VERSION     DAOSOR010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26320_length_3561_cov_4.309469"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1451
                     /locus_tag="PK710_22160"
     CDS             <1..1451
                     /locus_tag="PK710_22160"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT12500.1"
                     /translation="TDLAERLRVEARGLARLRHPNLVEVYDLGTALDGRIYFVMELLE
                     GATLRAMLSDQGRLSVSISVELMIQVLAGLQAAHGAGIVHRDVKPENVFVCRDGTVKL
                     LDFGVAKVMDAWIPNQPITVKGMTVGTPQYMSPEQAEGRALDGRTDVYAAALVLWEML
                     IGRPAYSDTDVYELLQAKWRGVMPLAEMGIGVSRGLSDVIVRASQPDLNERYPSATAF
                     QQALRIALVQSYLSSEAAEPKLRKKRDSLPSLEDMARSNRLMPPDVDATALTELVEAA
                     LAVDRDAPTRSHARALGPRGPTGTEILPAISPRPEARPMTESMQADQSVAVEDKACRS
                     RVSGNTLSLSHLPWVSWIAGTIAFLIPVMLAVLVGFWHFRKPSHAPEASSSMGAQGIS
                     RASAEPTDSLADAAAVPSWEENATIEGGVDVDSDAVVREDSSVSSATAGVKKPPIPKT
                     SARVEATMALPPSPALSSPPVSSERKMPKSGL"
     gene            complement(1508..2308)
                     /gene="dapB"
                     /locus_tag="PK710_22165"
     CDS             complement(1508..2308)
                     /gene="dapB"
                     /locus_tag="PK710_22165"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOT12501.1"
                     /translation="MSHTPRIALHGATGRMGAAMLSICRQANVPIVGAVAAPNTPDIG
                     LDLGEVHGTQPIGVLVTDNLSSALLGADVVVDFSHASAAQSLIKAVRSANLPLVSGTT
                     GFDADTHDALQRLGQQVPVLWASNFSLGIQVLAELVRHATQRLGPDFDVEIVEIHHRR
                     KVDAPSGTAIRLAQEAQASYPRQLRHGRHGMLGIRPQDELGISSVRGGDVVGDHTVHL
                     LGIGERLELTHRASSREVLARGALLAARCLPTMPPGVWQLADLLPSTS"
     gene            complement(2305..3213)
                     /gene="dapA"
                     /locus_tag="PK710_22170"
     CDS             complement(2305..3213)
                     /gene="dapA"
                     /locus_tag="PK710_22170"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOT12502.1"
                     /translation="MNQKLNLHGALTALVTPFSANGENLDIDAFEALLTAQLESGIDG
                     LVPCGTTGESPTLTDQEKRDIIAHTVRLAKGRVPIVAGTGSNDTQMSLRASQTALDLG
                     ADAIMLVMPYYNKPSQEGLFQHITTIATQIGNAPVVLYNIPGRSVVDLSNDTLARIVD
                     AAPNVVAVKDATGNVLRCQQVMHRFGNAITVLCGDDALTPAMMTNGALGVISVTSNLY
                     PDRVSAVCRAMKDSNIALARRLHLALLPVHDAMFVEPNPVPIKAALASKKRMHPTVRL
                     PLSPASRTTQDLVSRVTAAYEATEVA"
BASE COUNT          695 a          822 c         1274 g          770 t
ORIGIN      
        1 gtacggactt ggcggaacga ttgcgggttg aggcgagagg gcttgcgcgg cttcggcatc
       61 cgaatttggt ggaagtgtat gatttgggga cggcgctcga cggtcgaatt tattttgtga
      121 tggagctttt ggagggggcg acactgcgag cgatgctttc ggatcagggg cgtttgagcg
      181 tatcgatatc ggtggagctg atgatacagg tgctcgcggg attgcaggcg gcccacgggg
      241 cggggatcgt tcaccgcgat gtgaaaccgg agaatgtatt tgtttgtcga gatgggacgg
      301 tcaagctgct ggactttgga gtggcgaagg tgatggacgc gtggataccg aaccaaccca
      361 ttaccgtgaa agggatgacg gtgggaacgc cgcagtacat gtcgccggag caggcagaag
      421 gcagggcgtt ggacggtcgc acggatgtgt acgccgcggc gctggttctt tgggaaatgc
      481 tgattgggcg tcctgcttat tctgatacgg atgtctatga attgcttcaa gcgaagtggc
      541 ggggcgtgat gccgctggca gagatgggga tcggtgtttc tcgtggcttg agcgatgtca
      601 ttgttcgagc gagccaaccg gacctcaacg agcggtaccc atcggctacg gcgtttcaac
      661 aagcgctgcg aatcgctttg gttcaatcct atttgtcgtc ggaggccgca gaaccaaagt
      721 tgcgaaagaa acgtgattcg ttgccgagtc tcgaagacat ggcgagatcc aatcggttga
      781 tgccgccaga tgtcgacgcc accgcgctga ccgaattggt ggaagcagcc ttggcggtcg
      841 atcgcgatgc gcccactcgt tcgcacgccc gggcgttggg accgcgagga ccgacaggca
      901 ctgagatact tccagccatc tcaccgcgcc ccgaggccag gccgatgacg gagtcgatgc
      961 aggctgatca gtcggttgcc gtcgaggaca aggcttgtcg ttctcgcgtt tccggaaaca
     1021 cgctgtcgct ttctcacctc ccctgggtga gttggattgc cggaacgatt gcgtttctta
     1081 ttccggtcat gttggcggtg ctggttgggt tctggcactt ccggaaaccg tctcacgcgc
     1141 cggaagcttc gtcatcgatg ggtgcgcaag ggatctcacg agcaagcgcg gaacctacgg
     1201 attcccttgc cgatgcggct gctgtgccgt cgtgggaaga gaatgcgacg atagaggggg
     1261 gagtggatgt ggattccgat gctgtggttc gcgaggattc atctgtttcc tcggcgacgg
     1321 ctggtgtcaa gaagccaccg attcccaaaa cgtccgcgag ggtggaggca accatggcat
     1381 tgcccccttc gccagcgctg tcttccccgc cggtttcgtc ggagaggaag atgccgaagt
     1441 cggggttgtg acgtggggaa ggcaggttcc ccccaggttg tggcttaatg aataaaatag
     1501 tgtaaattca agatgttgaa ggtaaaaggt cggccagttg ccagactccg gggggcatgg
     1561 ttggcaggca gcgagcggcg agcagggcgc ctcgagcgag cacctcgcgg ctggaagctc
     1621 gatgggttag ttccaaccgt tccccgattc ccaacaggtg tacggtatgg tcgcccacga
     1681 cgtcgccgcc gcggacggat gagatgccga gttcatcctg aggccgaatt cccagcattc
     1741 catggcgccc gtggcggagt tgcctagggt aggaggcctg ggcttcttgg gccaggcgga
     1801 tagccgtacc gctgggggca tcgacttttc gtcggtggtg gatttcgacg atttccacat
     1861 cgaagtcggg tccgaggcgc tgggtggcat ggcggacgag ttcggcgagc acttgaattc
     1921 ccaggctgaa gttggaggcc cacaatacgg gaacctgttg gccgagtcgt tggagggcat
     1981 cgtgcgtatc cgcatcgaac ccggtggtgc cagaaaccaa gggaagattg gcggagcgta
     2041 cggccttgat gagggattga gccgcggatg cgtgagagaa gtccacgacg acatcggctc
     2101 cgagcaacgc ggaagagagg ttatcagtga cgagaacgcc gatgggttgg gttccgtgta
     2161 cttctcccag gtccaggcca atgtcggggg tattcggagc tgcgacggcc ccgacgatgg
     2221 gcacgttggc ctggcggcaa atcgagagca tggcggcacc catgcggcct gtggcaccgt
     2281 gcaaggcgat gcgcggcgtg tggctcatgc aacctccgtg gcttcgtagg cggcggtaac
     2341 gcgactgacc agatcctgtg tggttcgtga ggccggagat agcggaagtc gcaccgtggg
     2401 atgcatcctt tttttcgaag cgagagcggc tttgatggga accggattgg gttcgacgaa
     2461 catggcgtcg tgaacgggca gaagggctaa atgaagtcga cgggcgaggg cgatgttgct
     2521 gtctttcatg gctcggcaca cggcggagac ccgatcggga tagaggttgg atgtcacgct
     2581 gatgacgccg agggcaccgt tggtcatcat ggcaggcgtc aaggcgtcgt cgccacaaag
     2641 cacggtgatg gcgttgccga agcgatgcat gacttgctgg cagcgcaaga cattaccggt
     2701 ggcatctttg acagccacga cattaggggc ggcatcgacg attcgcgcga gggtatcgtt
     2761 ggacaagtcg acgacggatc gtcctgggat gttgtaaagg acgacgggag catttccgat
     2821 ttgggtggcg atggtggtga tgtgttggaa cagtccttct tgcgaaggtt tgttgtaata
     2881 gggcatgacg agcatgatgg cgtcggcacc caagtccaga gcggtttggg aggcgcgcag
     2941 gctcatttgt gtgtcattgg agccagtacc ggccacgatg ggaacgcgtc ctttggcgag
     3001 tcggacggta tgagcgatga tgtcgcgttt ttcctggtcg gtgagtgtgg ggctttcccc
     3061 ggtggttccg cagggcacca ggccatcgat tccactttcg agttgtgcgg tgagcagggc
     3121 ttcgaacgca tcgatgtcga ggttttcgcc gttggcggag aagggggtga ccaatgccgt
     3181 gagggcgccg tgcaggttga gtttttgatt catggggtgg gaggttggtc gttggcagag
     3241 ggtggatcaa gggtgcaatt gggaattggg tgggctaggg gcgagcgtcg tgggggcagg
     3301 ggtggggaag gattggctag ctatgattgg ttgatgaagc taacgatatt tgaattagtg
     3361 tcaatttgtt gcattagtaa gtagaagact atcacgttgt tatcggatag gcatgtaccg
     3421 aagcgaaaga taaaggcgta ggtaagcagg gcgttagggt ggagtgttgc aaatttgcac
     3481 aggcgttgcg acaaggtatt cgtgaatcgt tgtcgaatga ggggcgaaac aatgcgatag
     3541 gggaggcggg aacttcgacc a
//
LOCUS       DAOSOR010000595         3551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_26420_length_3551_cov_5.454233, whole genome shotgun sequence.
ACCESSION   DAOSOR010000595 DAOSOR010000000
VERSION     DAOSOR010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26420_length_3551_cov_5.454233"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PK710_22175"
     CDS             complement(<1..370)
                     /locus_tag="PK710_22175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOT12503.1"
                     /translation="MSSCLQFSIILCTRNRAELLSRALESMLSLDFPAEKFELCLVDN
                     GSTDATKTVVHAFAARAPFAVRYLFEGKPGLSIARNHGIQQALGTYLFFTDDDQLVDP
                     AVLREHLRVAQTYNVRAVQGA"
     gene            501..758
                     /gene="rpoZ"
                     /locus_tag="PK710_22180"
     CDS             501..758
                     /gene="rpoZ"
                     /locus_tag="PK710_22180"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOT12504.1"
                     /translation="MARVTVEDCLEREENRFALVVLAAARTRQLMKGASPLVRARNKA
                     AVVSLREIATGKVHFHRPSFEVVEEWLKTIPGAHVGFTEEG"
     gene            768..1598
                     /locus_tag="PK710_22185"
     CDS             768..1598
                     /locus_tag="PK710_22185"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOT12505.1"
                     /translation="MPRVPPIPSRTEAAVHDSNSWKAQGSLSHYTEPSYYDKCYANRH
                     HDIAFYETQTASAQTILEYGCGNGRIALPLARRGAHVTGVDLSIPMLESFRERLGREP
                     KPVRDRITLVHGDMRKKRLKRRFEVVLCTFNTFLHLYDRGDVEQFLACVRNHLEPEGK
                     FIMDTSLPLIEELARNPGRPYRVPRLKYPPTGQVVRYAEYFDYDPMSQVMRVTMRFEP
                     FDDPKAAWSVLLAHRQFHPQEIEALFHYNGFQVLSVHPNLDDGTTPLDSLGWVARVRR
                     "
     gene            complement(1609..2205)
                     /locus_tag="PK710_22190"
     CDS             complement(1609..2205)
                     /locus_tag="PK710_22190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12506.1"
                     /translation="MRLSWFFPALPLLLSAACTIIERPATSADAVEHSNDSNTRVEPN
                     DPEYSAGPSRERRGARPTARHRHGARPEGVGTYPSDPRQPAAPDTEKPDSPRRPLPDR
                     SKPQLEQQQDRSALERAPLHSLADGSSCSSGDECMSGICEGMGCGPRQGRCMPKERAC
                     TRDLRVYCGCDGQEFRASGRCPGRTFLHPGPCQVESDR"
     gene            2316..2732
                     /locus_tag="PK710_22195"
     CDS             2316..2732
                     /locus_tag="PK710_22195"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT12507.1"
                     /translation="MLPATSIAHIALYCTDLMSMERFYCDTLGYRVVWRPDSDNCYLS
                     RGKDSLALHVGPPTKAESRLDHFGMTLDCREHVQQWAQHLIASGIALEAPPRTHRDGS
                     TSFYVRDPEGNRIQFLHLGPGMTFAQEGENRGPSQT"
     gene            2990..>3551
                     /locus_tag="PK710_22200"
     CDS             2990..>3551
                     /locus_tag="PK710_22200"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT12508.1"
                     /translation="MNVRHVWLLGLLVGCKEPASTSEPPIGSPAVSTSSSSAAPVFSE
                     ASAPSAPTAMESVSASASSSASASGAAAETLCPEDMALVQGEHCSLVEQKCLEHALDK
                     EGKAIEAVCLRFEKPSKCNSTGRWHLRFCMDRYEYPNKVGEKPMVLVDFHEATRLCQE
                     QGRRLCTPAEFNFACEGEDMLTYATGF"
BASE COUNT          784 a          959 c         1059 g          749 t
ORIGIN      
        1 tcgctccttg tacggcacga acgttatagg tttgggccac acgcagatgt tctcgcaaca
       61 cggcgggatc cacgagctgg tcgtcgtctg tgaaaaacag atatgttccc aaagcctgct
      121 gaatcccgtg atttctggcg atggaaagcc cgggtttgcc ctcgaataga tagcgaacgg
      181 caaagggagc ccgggcagca aacgcgtgga cgaccgtttt cgtggcgtcg gtcgaaccgt
      241 tgtcgaccag gcataattcg aatttttccg cgggaaaatc gagggacaac atgctttcga
      301 gggcccggga caacagctcc gcgcggttgc gtgtacatag gatgatggaa aattggaggc
      361 aagacgacat ggtattcacg gcgtgataag ccttgcgccc gtttcgagca agggcgagag
      421 tcgaacaatc gcccttgcat gacacgacgg agaagactag gctcactgcc tcttttcgaa
      481 aggtttttag gagctacacc atggcccgcg tcactgtcga agattgcctg gagcgtgagg
      541 aaaaccgttt tgcccttgtg gttctcgcag cggcccggac acgtcagctc atgaaagggg
      601 cctcaccgct ggttcgggcg agaaataagg ccgcggtggt gagtctacgc gagattgcca
      661 cgggaaaagt ccatttccac cgaccctcct tcgaggtcgt ggaagaatgg ctaaaaacca
      721 ttcctggggc ccacgtcgga ttcaccgaag agggctgatc cgaccccgtg ccccgcgtac
      781 cgcctatccc gtcgcgcacc gaagctgccg ttcacgacag caactcgtgg aaagcccaag
      841 gctctctatc ccattacacc gagccctcct attacgataa atgctacgcg aatcgtcacc
      901 atgacatcgc gttttacgaa acgcagaccg cttcggcgca aaccattctc gagtatggtt
      961 gcggcaacgg acgcatcgcg ttgccgctgg cccgtcgagg cgcccacgtt acgggagtcg
     1021 acctttccat ccccatgctc gagtcctttc gagaacgtct gggtcgagaa ccaaagcccg
     1081 tacgcgatcg gattaccctc gtccatggag atatgcgaaa aaaaaggtta aaaaggcgtt
     1141 ttgaagtcgt gctttgcacg ttcaacacgt ttttgcacct gtatgaccgg ggcgatgtag
     1201 agcaatttct ggcttgcgtg cgaaatcacc tcgagcccga aggcaagttc atcatggata
     1261 cgtcgctgcc cttgatcgaa gagctggccc gcaacccggg acgaccctat cgcgtaccga
     1321 gattgaagta tccccccacc ggacaagtcg tgcgctatgc agaatacttc gactacgatc
     1381 cgatgtccca ggtcatgcgc gttacgatgc gattcgaacc tttcgacgat cccaaagcgg
     1441 cgtggtccgt gctgcttgcc catcggcagt tccatcccca ggaaatcgaa gcgctttttc
     1501 attacaacgg gtttcaggtg ctatccgttc atcccaatct cgatgatggg accaccccgt
     1561 tggattcctt gggctgggta gctcgcgttc gccgctgatg attcgagatt atcgatcgga
     1621 ttcgacctgg cacggaccgg gatgcaggaa cgtacggccg ggacatcgac cgctcgcacg
     1681 gaattcctgg ccatcacagc cgcaatatac acgaaggtct ctcgtgcagg cgcgctcttt
     1741 gggcatgcag cgtccttgtc gtggaccaca gcccatcccc tcgcaaatcc cactcataca
     1801 ctcatcacca gaagaacaag aggaaccgtc ggcaagagaa tgcaaaggag cacgttccaa
     1861 cgcagagcgg tcttgctgtt gctcgagctg gggcttggaa cggtcgggaa gaggccgtcg
     1921 tggggagtct ggcttttccg tgtcgggagc ggcgggctgg cgggggtcgc tcggataggt
     1981 tccaacaccc tctggacggg caccgtgtcg atgcctggcc gtaggacgag caccgcggcg
     2041 ttcacgagaa gggccggcgg aatattctgg atcgttgggt tcgaccctcg tatttgaatc
     2101 gttggaatgc tccaccgcgt ccgctgaagt tgccgggcgt tcgatgatcg tgcaagcggc
     2161 tgacaaaagc aggggcagcg cggggaaaaa ccatgaaaga cgcatacatc acctcgaaat
     2221 catgactgtg ggcagccgat gtagggtttg acgaagcagc cagtggattg tttcaccctt
     2281 gcgtttcgag accatcgccc cagagtcggc acaccatgtt acccgccacg agcattgccc
     2341 acatcgcatt atattgcacg gacttgatgt cgatggaacg attctactgc gacacgctcg
     2401 gatatcgcgt cgtgtggcgc cccgattcgg acaattgcta tctgtcacgg ggcaaagaca
     2461 gcttggcgct ccacgtcggg ccgcccacga aagcggaatc gcggctggat cattttggaa
     2521 tgacgttgga ctgtcgtgag catgtgcagc agtgggcgca acacctgatc gcgtcgggga
     2581 tagcgctgga agcgccccct cgaacgcatc gtgatggctc cacgagtttt tacgtgcgtg
     2641 accctgaagg caatcgcatt caatttcttc atctcggacc gggcatgacg tttgcgcaag
     2701 agggtgaaaa tagggggcca tcgcagacgt aacgggcttt ggcagaagga caatgcgcag
     2761 cccaggccga tgggtagcga gcatgagttt tcggagatat tttttcgaaa gcgaggggga
     2821 cggagcgatt cagaaggctg agccgctcga cggaacagtg aacgggtggg gaagccgcgc
     2881 ggcatgacta gcataagtat gtgaaattat acgatatttt tatgtctcga caagatggac
     2941 tccaggaatg gatattcagg tgcaagctac tgtattacgt tggtaagcga tgaacgtgcg
     3001 gcacgtatgg ctccttggtt tgctggttgg gtgtaaagaa cctgcgtcga caagcgaacc
     3061 gccgattggc agccccgcgg tttcgacaag ttcgagttca gcggcgcctg tgttttctga
     3121 agcatcggct ccttccgcgc ccacggcgat ggaatccgta tcggcatcgg cgtcttcttc
     3181 cgcttcggcg tctggggccg ctgcggaaac gctatgtccc gaggacatgg cattggtgca
     3241 aggggagcat tgctcgcttg tggagcaaaa gtgtctcgaa catgctctcg acaaggaagg
     3301 caaggcgatc gaggcggttt gtctgcgttt tgaaaaacca agcaagtgca attcgacagg
     3361 ccggtggcac ctgcgatttt gcatggatcg atacgagtac cccaacaagg ttggggaaaa
     3421 gccaatggtc ttggtcgact ttcatgaggc cacacggtta tgccaagagc aagggcgacg
     3481 gttgtgtacg ccagcggagt tcaacttcgc ttgcgagggg gaagacatgc ttacgtatgc
     3541 cacggggttc g
//
LOCUS       DAOSOR010000596         3550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_26437_length_3550_cov_2.966237, whole genome shotgun sequence.
ACCESSION   DAOSOR010000596 DAOSOR010000000
VERSION     DAOSOR010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26437_length_3550_cov_2.966237"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="PK710_22205"
     CDS             <1..801
                     /locus_tag="PK710_22205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12509.1"
                     /translation="SSSDGKIIRLPMDGGIPQEVAGGLDNITSLAFGDGNVFWTMCGT
                     IEHVQRCRITQTPTSGGKRNLVFDAGPNQVAYAVWAGTHLLWAEATNKSVMKTDTKQP
                     KPAALTATGDVTSMVSRDNDIFWSEGRALAPDGAIMRSSLSGPALAVSKGRRFPRHLL
                     VDGDRLIWVENSETEAGSVLHAIMTVEANGGEPKTIVKDLSHVEGIAVGGTKLLIMTS
                     DTLTYVPKTGGESAVLVADLKSPAGPVATESHAYWISNDKIWRISLQP"
     gene            996..2171
                     /locus_tag="PK710_22210"
     CDS             996..2171
                     /locus_tag="PK710_22210"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOT12510.1"
                     /translation="MSWTAPSSLPLSSDGNELPVLPRFFTSLASLSQGERKQPVRVSW
                     LGDSHTQPDIWTQAVRTPFQERFGSGGLGFVHVGWEKWGYRHGMAELSVQGQWGIEPP
                     RLLSVEPFDDGVLGLGGVRLVPQKGATASVQARPGTLQGRARWELAVRFRHGRGALRI
                     IPSEGSPWQVHADAQHTHVQDIGWVTTEPDSGFSMEVVEEGVEVFGVVAELEASGGVV
                     FDVLGLNGARVIHALGWNETAWQQRLQRRAPDLLVLAYGTNEAGIRNLSMQGHTQDLV
                     ALIDRARIASPNADCLIVGTMDRGGDAMSEKVHVLNEAQKQAAQLRGCAFWSAQVAMG
                     GKASMQRWQKENPPYATSDRVHLTVRGYQKLGNMLATDILQAFDAGTVRMVSESITN"
     gene            complement(2365..>3550)
                     /locus_tag="PK710_22215"
     CDS             complement(2365..>3550)
                     /locus_tag="PK710_22215"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOT12511.1"
                     /translation="NEIPTLSLEDARERGLLRPVDNVITERYLRTIEQLSRHSQPRSD
                     LRIVYTPMHGVGLATAMEALKRAGFDQVVPVASQAAPNGEFPTVHFPNPEEPGAMDLA
                     FATAKETGADLILANDPDADRLAVAVPNRDGDGFLQLSGNQVGVLLAHYYLAYDPSPP
                     KDRLFVTTIVSSPMLGAMARQEGARYEETLTGFKWIANRAMQVEADTGTTFIAGYEEA
                     LGYTIGTAVRDKDGVSAVAVFAEMTAYLRSQGRSILDELELMYRKYGLYVSRQVSRWH
                     RGLDGAQRIQQIMAKLRAAPPSRLEDFDVVAVRDYQQQLRTQRDGTRTTLDLPKSNVL
                     SFELEGGSRIVARPSGTEPKIKFYFDHREVMHPEESLPSAEKRANERLDRLVNAFLQA
                     AE"
BASE COUNT          769 a          978 c         1067 g          736 t
ORIGIN      
        1 tcgtcttccg atggaaaaat cattcgtttg cccatggacg gcggcattcc tcaagaggta
       61 gccggcggat tggacaatat tacttcgctc gcttttggcg atggcaacgt gttttggacg
      121 atgtgcggca cgatcgaaca tgtgcagcga tgtcgtatca cccagactcc aacgtcggga
      181 ggcaagcgga acctcgtctt cgatgcgggt cccaatcaag tcgcgtatgc cgtatgggcg
      241 ggcacccatt tgctctgggc agaggctacc aacaaatccg tcatgaaaac cgataccaag
      301 caaccgaagc ccgcggcgct cacggcaacg ggtgatgtga cgagcatggt atcacgcgac
      361 aacgacatat tctggtccga aggacgagcc ttggctcccg acggggcgat catgcgatcg
      421 tcgttatccg gtccggccct tgccgtatcg aaaggacgac gatttccgcg tcatctcctc
      481 gtggatgggg atcgattgat ttgggtggag aattccgaaa ccgaagcagg gtccgtgtta
      541 cacgccatca tgacggtcga ggccaatggc ggtgagccca aaacgatcgt caaggatttg
      601 tctcatgtgg aaggtatcgc ggtgggaggc acgaagctat tgatcatgac ttcggatacg
      661 ttgacgtatg ttccgaaaac ggggggcgaa agcgcggtat tggtggctga tttgaaatcc
      721 cccgcgggtc cggtcgctac cgaatcccac gcttattgga tttcgaacga caaaatatgg
      781 cggatctccc ttcagccctg aagcgtcgaa ttcccgcttt actagggata tggctcagcg
      841 cttccgtctg cgcgtgtgaa acgacatccc cacggccttc cgtgtcgacg ctcccggtat
      901 cacaccccag aagcgatctt cataagcagc aaaccgtctc gtctgcgagc ctgccaagcg
      961 agactctgcc cccgcgagat cagcagccat tcacggtgtc gtggacggcg ccttcgtcgt
     1021 tgcctctctc ctccgacggc aatgaattgc cggtacttcc caggtttttc acttccttgg
     1081 cctcgctatc acaaggagaa aggaagcagc cggttcgggt gtcatggctt ggggactcgc
     1141 atacacaacc ggatatttgg acccaggccg tgcgaacgcc cttccaagag cgatttggct
     1201 ccggagggct gggattcgtg catgtgggat gggaaaaatg gggctatcgg cacgggatgg
     1261 ccgaactatc cgtacaggga caatggggca tcgaaccacc acgtcttctt tcggtggaac
     1321 ctttcgacga tggggtgttg gggttgggcg gcgtgcgcct cgtgccgcaa aaaggggcga
     1381 cagcctcggt gcaagcaagg ccaggaacgc tccaaggacg cgcgcgatgg gaattggccg
     1441 tgcgatttcg tcacggacga ggcgcgctgc gaatcattcc ctccgagggc agcccttggc
     1501 aagttcacgc cgatgcgcaa cacacccacg ttcaagacat tggatgggtc accacggaac
     1561 cggatagtgg tttttcgatg gaagttgtcg aggaaggtgt cgaagttttc ggtgtggtgg
     1621 cggagttgga agcatcgggg ggcgtggttt tcgatgtgct cggattgaat ggcgcgcgcg
     1681 tgattcatgc gctcggctgg aacgaaaccg cctggcaaca gaggctgcaa cgccgcgcac
     1741 cggaccttct ggtgctggcg tatggcacga atgaagcggg tattcgcaat ctatccatgc
     1801 aggggcatac ccaggacttg gttgcgctga tcgatagagc acgcatcgct tctcccaacg
     1861 cggattgcct gatcgtgggc accatggatc ggggaggcga tgcgatgagc gaaaaggttc
     1921 acgtgttgaa cgaggcgcaa aaacaggcgg cgcaattgcg cggctgcgcc ttttggagcg
     1981 cgcaagtggc catgggaggc aaagcaagca tgcaacgatg gcaaaaagaa aatccccctt
     2041 atgccacctc cgatcgcgta cacctgaccg tccggggcta tcaaaaactc ggaaatatgc
     2101 tggccacgga catccttcag gccttcgacg cgggaaccgt gcgcatggtt tctgaatcta
     2161 taaccaattg attttattga aacaataaga caggaggatt cgaccggggc atcgatttcc
     2221 aagccatgaa cgatgcgcca agacaactct gttcacgtca tgaaaacgcg aactttcaca
     2281 cgggcgcgtg acaagcgaag cgatgagcac gggtgggata ccgaggatga ggttcgaatc
     2341 gtcgtcgtgc agccacgagc ggactcattc cgcagcttgc aaaaaagcat tcaccaggcg
     2401 atcgagacgc tcgttcgctc ttttttccgc ggacggcaat gactcctcgg ggtgcatgac
     2461 ttcccgatga tcgaagtaaa acttgatctt gggttcggta ccgctcgggc gtgccacaat
     2521 ccggctgcca ccttccaatt cgaaagaaag cacgttgctt ttcggaaggt cgagtgtggt
     2581 tctggtacca tcacgctgcg ttcgaagttg ctgctgatag tcccgcaccg cgaccacatc
     2641 gaaatcctcc agcctcgatg ggggagccgc acgaagcttc gccatgatct gttggattcg
     2701 ctgtgcgccg tccagaccac gatgccatcg actcacctgg cgactgacat acaacccgta
     2761 cttgcgatac atcagttcga gttcatcgag aatgcttcgc ccttgcgaac gaagataggc
     2821 ggtcatttcg gcgaaaaccg cgacggcgct cacaccatct ttgtcacgca cggcggttcc
     2881 gatcgtatag ccgagcgctt cctcgtaacc ggcaatgaag gtggttcccg tatccgcctc
     2941 gacttgcatc gcgcgattgg cgatccactt gaacccggtc agcgtttcct cgtatcgcgc
     3001 tccctcctgg cgcgccatcg caccgagcat gggtgaagat acgatggtcg tgacaaaaag
     3061 ccggtctttg ggcggcgaag gatcataagc gaggtagtaa tgggcgagca gtacgcccac
     3121 ctggttgccg gacaactgga gaaagccatc cccatcccgg ttgggcacgg ccacggcgag
     3181 acgatcggca tccgggtcat tggctagaat caagtccgcg ccggtttctt tcgcggttgc
     3241 gaaagccagg tccatcgctc ccggttcctc gggattggga aagtgaaccg tgggaaactc
     3301 gccattcggg gcggcttgcg aagccaccgg aacaacctgg tcgaatccgg cccgtttcaa
     3361 agcttccatg gccgtggcaa ggcccacgcc atgcatgggc gtatagacga ttcgcaagtc
     3421 ggagcgcggt tgcgaatgcc gcgaaagctg ttcgatggtg cgcagatacc gttcggtgat
     3481 gacgttatcg acggggcgta acagcccgcg ttcacgagcg tcttcgagcg ataaggtagg
     3541 gatttcattg
//
LOCUS       DAOSOR010000597         3540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_26528_length_3540_cov_4.503300, whole genome shotgun sequence.
ACCESSION   DAOSOR010000597 DAOSOR010000000
VERSION     DAOSOR010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26528_length_3540_cov_4.503300"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PK710_22220"
     CDS             <1..450
                     /locus_tag="PK710_22220"
                     /inference="COORDINATES: protein motif:HMM:NF022059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOT12512.1"
                     /translation="DNRPCKAIQTGGPSGGCIPARFIDLPVDYESLREKGSMMGSGGM
                     IVMDDTSDMVDVARFFMEFCVGESCGKCVPCRVGTAQMNDLLTKIIDGKATKHDIVHL
                     EELCELLRDTSLCGLGQSAPNPVVSTLTHFRQEYLDRLVETDEEAAS"
     gene            447..1166
                     /gene="hoxU"
                     /locus_tag="PK710_22225"
     CDS             447..1166
                     /gene="hoxU"
                     /locus_tag="PK710_22225"
                     /inference="COORDINATES: protein motif:HMM:NF005745.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bidirectional hydrogenase complex protein HoxU"
                     /protein_id="HOT12513.1"
                     /translation="MSPKVRIKTLKIDGRDIGAREDETILQVARENGIQIPTLCHLDG
                     LEPIGACRLCMVEVRGSSRLLPACVTRVEEGMEVTTKSPRLIRHRRMILELLFAERNH
                     VCSVCVVNGKCELQSLAVALGMTQVRTPYRFPRFELDASHPRFVNDHNRCVLCARCVR
                     VCDEIEGAHTWDIMGRGVNGRVITDLNEPWGESNSCTGCGKCVHVCPTGALSEKGTLR
                     EDAGKQRDFLPYLTLMREVRR"
     gene            1163..1696
                     /locus_tag="PK710_22230"
     CDS             1163..1696
                     /locus_tag="PK710_22230"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12514.1"
                     /translation="MTKARLATVWLDGCSGCHMSLLDMDEKILEVAKVADVVYSPLVD
                     TKDFPENVDVALVEGAVSSEDDLEKIQRIRKNSRFLVALGDCAVTGNVAAMRNLFGVR
                     AVLDRSYRLTSVPAGSPPDQIVPRLLSRVRPLHEVVAVDLTVPGCPPSAGVIHHVVTE
                     LLAGRTPDVTDLTRFGA"
     gene            1709..3133
                     /locus_tag="PK710_22235"
     CDS             1709..3133
                     /locus_tag="PK710_22235"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOT12515.1"
                     /translation="MAQNIVIDPVTRIEGHAKITIQLDEHGFVHDAHFHVTQVRGFEK
                     FCEGRPFYEMPGLMARICGICPVSHLVASSKACDAIMAVSIPPVAENLRRLMNLAQIL
                     QSHALNAVHLSGPDLALGMDAPAAERNIFGLVEKHPGLAQDGISLRSVGQHIIETLGN
                     KRVHPAWIVPGGVNSPLSAQQRDHILSLLPDALVKAQRLVDWFKSIMDSFSEEIRVFG
                     NFPSLFMGLVDEEQNLEHYHGSLKVIDFQGKTLVPSLPVDRYGDYIAEAVEPWSYLKS
                     PYFKAKGYPNGIYRVGPLARLNIVEGCGTRWADQELAVFKDMDQPVVSSSFHYHYARL
                     IEMLFCIERMQQLLEEPDILDTHVRAYASANARRGIGVSEAPRGTLIHDYEVGPNGLI
                     KSANLIIATGNNNLAMNQAVLQVAKRFVRGPDVTDGMLNRVEAVIRAYDPCLSCSTHA
                     LGKMPLEVTIVDSDGHPVRVCTNP"
     gene            3225..>3540
                     /locus_tag="PK710_22240"
     CDS             3225..>3540
                     /locus_tag="PK710_22240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12516.1"
                     /translation="MLRSLLIASLAMLTVSCSEDSSTVEGGSSGSSGADANVEDTQTD
                     VVDSGVEGGKDAPEEASSTDVSADTLADTEPETSPPDAEPDVVDAGTGQARCGATPYD
                     WIT"
BASE COUNT          750 a          986 c         1002 g          802 t
ORIGIN      
        1 gacaaccgcc cctgcaaagc gattcagacc ggaggccctt cgggaggttg catccccgcg
       61 cgcttcatcg acttgccggt cgactacgaa tcccttcgcg aaaaaggttc gatgatggga
      121 tccggaggca tgatcgtcat ggatgacacg tcggatatgg tcgacgtagc ccgatttttc
      181 atggagtttt gtgtgggaga gtcctgtggc aaatgcgtcc cttgccgcgt gggaacggca
      241 cagatgaatg accttctcac gaaaatcatc gacggaaagg ccaccaaaca cgatatcgtt
      301 cacctcgaag agctatgtga gttgttgcga gacacgagtc tttgcggtct aggccaatcc
      361 gctcccaatc ccgtggtctc caccctgact cacttccgcc aggagtatct cgatcgtctc
      421 gtcgagacgg acgaggaggc tgcatcatga gccccaaagt tcgcatcaaa acgctaaaaa
      481 tcgatggccg ggacattggt gcgcgcgagg atgaaaccat cctgcaggtg gcccgcgaaa
      541 acgggattca aattcccacg ttatgccacc tcgatggcct cgaacccatt ggagcttgtc
      601 gcttgtgcat ggtggaagtg cgcggatctt ctcgtctttt acccgcctgt gtgactcgcg
      661 tggaagaagg catggaagtc acgaccaagt ctccccgatt gattcgccat cgcagaatga
      721 tcctcgaact gctttttgct gaacgcaacc acgtttgctc cgtttgtgtc gtcaatggca
      781 aatgcgagct tcagtccctc gccgtggcgc ttggcatgac gcaagtacgc acgccctatc
      841 gttttccccg ctttgaattg gatgcctcgc acccacgttt cgtcaacgac cacaaccggt
      901 gcgtgttgtg tgcccgttgc gttcgcgtct gcgacgagat cgagggagcg catacatggg
      961 atatcatggg tcgaggtgtc aacggtcgtg tgattacgga tctgaatgag ccctggggag
     1021 agtcgaatag ctgcacgggt tgcggaaagt gtgtccatgt ttgcccgacc ggtgcgttgt
     1081 cggaaaaggg cacgttgcgt gaagacgccg gaaaacagcg tgattttctg ccttacctca
     1141 ccttgatgcg ggaggtgcga cgatgaccaa agcacggctt gcgacagtgt ggctcgatgg
     1201 ttgttcgggc tgtcacatgt ctttgttgga catggatgaa aaaattcttg aagtcgccaa
     1261 ggtcgcggat gtcgtgtaca gcccgcttgt cgacaccaag gactttcccg agaacgtgga
     1321 cgtggcgttg gtggaaggag cggtatcgtc cgaagatgat ctggaaaaaa tccaacggat
     1381 tcgaaaaaac tcgcgatttc tcgtagcgct cggagattgc gcggtgaccg gcaacgttgc
     1441 ggcgatgcgc aatctatttg gcgttcgtgc cgttctggat cggtcctaca ggctaacgtc
     1501 ggttcccgcg ggaagcccgc ccgaccagat cgttcctcga cttctatcgc gcgttcgtcc
     1561 gctccatgag gtcgttgcgg tcgacctcac ggtgccgggg tgtcctcctt cggcaggggt
     1621 cattcaccat gtcgtcaccg aattgctggc gggaagaacg ccggatgtaa ctgacttgac
     1681 gcgctttggc gcatgaggat gtgtgtccat ggcccagaac attgtcattg acccggttac
     1741 gcgcatcgaa ggacacgcca agatcacgat ccaactcgat gagcatggtt tcgtccacga
     1801 cgcgcacttt cacgtcacgc aggttcgcgg tttcgaaaaa ttttgcgaag gcaggccctt
     1861 ttacgagatg cctggcttga tggctcgcat ctgtggaatc tgccccgtca gccatctggt
     1921 agcttcgtcc aaagcctgcg atgcgatcat ggcggtgagc atacctccgg tagcggaaaa
     1981 cctgcgcaga ttgatgaatc ttgcgcagat tctccaatcc catgcgctca acgccgtaca
     2041 tctttcgggg ccggacctgg ctcttggcat ggatgctcca gccgccgaac gcaatatctt
     2101 cggcctggtc gaaaagcatc caggcctggc gcaagatgga atcagcttgc gatcggtggg
     2161 gcaacatatc attgaaaccc ttggaaataa gcgggttcac ccggcatgga tcgttcctgg
     2221 aggcgtgaac tctcctcttt ccgcacaaca gcgagaccat atcctctcgc tgctccccga
     2281 cgctttggtg aaagcacagc gactcgtcga ctggttcaaa agcatcatgg acagcttttc
     2341 cgaagagata cgcgtctttg gaaattttcc ctccctgttc atggggctcg tcgatgaaga
     2401 acagaatcta gaacactacc acggatcact caaagttatt gattttcaag ggaaaaccct
     2461 agtaccttcg ttgcctgtcg atcgttatgg cgactatatt gccgaagccg tggaaccttg
     2521 gtcctacctg aagtccccct acttcaaggc caaaggatac ccgaacggca tctaccgcgt
     2581 gggtcccctc gcccggctca atatcgtcga aggatgcggc acgcgatggg ccgatcaaga
     2641 gcttgcggta ttcaaggaca tggatcaacc ggtcgtctcc agttcttttc attatcatta
     2701 cgcccggttg atcgagatgc ttttctgcat cgaacgcatg cagcaactcc tcgaagaacc
     2761 ggatattctc gatacgcatg tgcgagcgta cgcctcggcc aatgccaggc gcggtatcgg
     2821 agtgtccgaa gcgcctcgcg gcacgttgat ccatgactac gaagtcggtc ccaatggtct
     2881 catcaaaagc gccaacctca tcattgccac gggcaacaac aatctggcga tgaaccaggc
     2941 ggtgttgcaa gttgccaaac ggttcgtgcg cggccccgat gtcaccgatg gcatgctcaa
     3001 tcgtgtcgaa gcggtgattc gagcttacga cccctgtcta tcctgctcta cccacgcgct
     3061 cgggaaaatg cctctggaag ttaccattgt ggactcggac gggcacccgg tccgggtctg
     3121 caccaatcct tgatgttgtg acaacagcgc tgcatatcgc gcgcttttcc gctccccgac
     3181 aaattgtgct tgcatgactg tcgatcacgg ggtgaacttc agtcatgctc agaagtttgc
     3241 tcattgcttc attggccatg ctgaccgtct cttgctccga ggactcttct accgtggaag
     3301 gagggagttc gggttcgagt ggagccgatg caaacgtgga agacacgcaa accgatgttg
     3361 tggacagcgg cgtggaaggc gggaaggatg cgcccgagga agcttcttcg acggatgttt
     3421 ccgcggacac gctcgcggat accgagccgg agacttcgcc cccggacgcc gagccagacg
     3481 ttgtagatgc tggcacaggg caagcacgtt gcggcgcgac gccctacgat tggatcacgt
//
LOCUS       DAOSOR010000598         3531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_26613_length_3531_cov_4.953395, whole genome shotgun sequence.
ACCESSION   DAOSOR010000598 DAOSOR010000000
VERSION     DAOSOR010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26613_length_3531_cov_4.953395"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PK710_22245"
     CDS             complement(<1..469)
                     /locus_tag="PK710_22245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT12517.1"
                     /translation="MSIVFAMLSNMGCSNRPVVVRIIDGQTVQGRFVHPEAYALVMEG
                     QLAEQRGWLAQAERLYLAALRYQPNDASILVRLGALRCAWNQQTVPAAQKTFADALKV
                     DRFLVSAYVERGRCALRHGMTGQAEQDARRALSLAPSDWTVTALLADVLEKGGK"
     gene            complement(496..1941)
                     /locus_tag="PK710_22250"
     CDS             complement(496..1941)
                     /locus_tag="PK710_22250"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT12518.1"
                     /translation="MTEPPRNTKSPSPHGDITGSDFLFHLYRGAELLQENLVHEAKAE
                     IENALSLQPLEPQGQDLLAQVYFRLGLYPRAIAIYEQLASNFPDELTPRINLSLCYLK
                     TGQPERAREHLEHVVVSEPSRQRAWAYLGLAFERLGDYEKARAAFDRAGYSSMARRME
                     VLVDSSSAQHEAPSSSKSDPNLHEVRRVAEAAFHELDASDGAFCLASSTPQEDSKTPG
                     TWVEVELGRPDKKAITAIAPAPFSESSSFAQPTASAPLEPEQPVRHALPLQAWTQAYN
                     GVFSRNHRSITHADGTLLVSVHNEFATREDLVEAFVCFDIPQARENIPREQGGDPSLP
                     LGGSNRPLLRVQQTRHIVLHPRVDRVLQAVMLEHESFMIREECLGAFDLDLSHDYGRL
                     ASGLGDTISLVHLRGTGLVVLDLPTAFMALEVQEKQGCVLRCSSLIGWTGRIVARCLP
                     AHESPAAMRGWTGISGQGTVFLASSHNTLFS"
     gene            complement(1981..2691)
                     /locus_tag="PK710_22255"
     CDS             complement(1981..2691)
                     /locus_tag="PK710_22255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12519.1"
                     /translation="MRQSDTHRAPGKKNTFRYLLPFVVLTMVGGCGVRGTPVRGPAET
                     LRGYADALDQNRIEDAYAMLSDEAKREMPLDAFRRMVQENPNEMRDVAEALRRPGSVP
                     VVTATIDTPDGQALLLRYESGRWRVDVSAVDLYSQNTPRQAVTSFVRAFERGRYDVLM
                     RFVPEAKKPGLDASKLKAAWEGSQKQEMQTLIAAVKSALPTATFEEAAGRATMPFGAV
                     GTVQLVQENGVWKIEDFD"
     gene            2884..>3531
                     /locus_tag="PK710_22260"
     CDS             2884..>3531
                     /locus_tag="PK710_22260"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOT12520.1"
                     /translation="MSRGPSAAQEGISIPILPLRNSVLFPMSVVPINVGRPRSVRLIE
                     DALGMERATIGILTQFASDTDEPTFRDLYKIGTLARIVQVIRVGPSVYTVKISGIARF
                     RVLSGQGLEPFMRAQVQRIIDPVDHDEQSDQLSAHLRESARTLLTRNPALPQELNGIL
                     ENVREPGALADLLVASFPAEFAPVDERQQVLETFDIKKRMMLVQEMIDRQLKVLKV"
BASE COUNT          764 a         1015 c          998 g          754 t
ORIGIN      
        1 gtttaccccc tttttcgagc acgtctgcaa gcaacgcggt aaccgtccag tcggacggag
       61 ccagactgag ggcccggcgt gcatcctgct ctgcttgccc cgtcattccg tggcgcaaag
      121 cgcaacgacc gcgctcgaca taggccgaaa ccaaaaacct atccactttc agcgcatcgg
      181 caaacgtttt ctgtgcggct ggcaccgttt gttggttcca agcgcaccgt agcgcaccca
      241 gccgtacaag gatcgaagca tcgttcggct gataacgaag agcagcaaga tataagcgct
      301 cggcttgtgc caaccatcct cgctgctcgg caagttgtcc ctccatcacc aacgcatacg
      361 cttcgggatg tacaaatctc ccttgaacgg tctgtccgtc gatgatgcgc acgacaacgg
      421 gacggttcga acagcccatg ttcgaaagca tcgcgaacac gatgctcata ccccacgaaa
      481 agacccagcg agcgctcatg aaaacaacgt gttgtgggat gaagcgagga aaaccgttcc
      541 ttggccggaa atgccagtcc atcctcgcat cgccgccgga gactcatggg cgggaaggca
      601 tcgcgccacg atgcgtcccg tccagccaat cagcgatgag cagcgcagca cgcagccttg
      661 tttttcctgg acttcgaggg ccatgaatgc ggttggcagg tcaaggacaa caagccctgt
      721 gccccgtagg tgaacgagag atatcgtatc tcccaatccc gacgcgaggc gtccatagtc
      781 atgggatagg tcgagatcga aagctccgag acattcctct cggatcatga agctttcgtg
      841 ttcgagcatc acggcttgaa gcacgcggtc gacacgagga tgtaagacga tgtgccgagt
      901 ttgttgaaca cgtaacagag gtctgttcga tccgcccaga ggcagcgatg gatcgccgcc
      961 ttgctcccgt ggaatgtttt cacgagcttg ggggatgtcg aaacagacga aggcttcgac
     1021 caggtcttct cgcgtggcga actcgttgtg gacggagacg agcagtgtgc catcggcgtg
     1081 tgtgatactg cgatgattac gcgaaaacac gccgttgtat gcctgcgtcc aggcttgcag
     1141 cgggagagca tggcgaacgg gctgttcggg ctcgaggggg gcggaagccg ttggttgcgc
     1201 gaatgaggac gattcggaaa atggcgcggg agcgatagcg gttatcgctt tcttgtccgg
     1261 gcgtccaagc tcgacttcaa cccaagttcc aggcgttttc gaatcctcct ggggtgtgga
     1321 agaagcaagg caaaaggctc cgtccgaggc atcgagttca tgaaaagccg cttcagcaac
     1381 ccgacgtact tcgtgcaagt tcggatcgga tttcgaggag ctaggcgctt cgtgttgtgc
     1441 agaggaagaa tcgacaagga cttccattcg cctcgccatg ctgctgtagc cggcgcgatc
     1501 gaaagcggcg cgcgcttttt cgtaatctcc aaggcgctcg aaagcgaggc ctaaataggc
     1561 ccacgctcgc tgacgagagg gttccgaaac gacgacgtgt tcgagatgct ctcgagcacg
     1621 ttccggctga cctgttttca ggtagcataa ggaaagattg atacgaggcg tcaattcgtc
     1681 gggaaaattg ctcgcaagtt gctcataaat cgcgatggcc cgagggtata gccccaaacg
     1741 aaagtagacc tgggcaagca agtcctgccc ttgcggttcg agcggttgaa gcgataaggc
     1801 gttttcgatt tccgccttgg cctcgtgaac gaggttttcc tgaagcagtt ctgctcctcg
     1861 gtataggtga aagagaaaat ccgatcccgt gatgtcgccg tggggcgacg gacttttcgt
     1921 gttgcgagga ggttctgtca tggctcagta cctgaagcgc tcgttcgaag gccgatccgt
     1981 tcagtcgaag tcctcgattt tccatacccc gttttcttgc acgagttgaa ccgttcccac
     2041 cgcgccaaac ggcatcgtgg ctcggcctgc cgcttcctcg aacgttgccg tgggcaaagc
     2101 gctttttacc gcggcgatga gcgtttgcat ctcctgtttc tgacttcctt cccaagccgc
     2161 cttgagcttg gatgcatcca aaccaggctt tttcgcttcc ggtacgaacc gcatgagaac
     2221 gtcatagcga ccccgttcga aagcacgtac aaacgacgtt accgcttggc gaggagtgtt
     2281 ttgcgaatac aaatcgaccg ccgatacgtc gacgcgccaa cgacccgatt cgtaccgaag
     2341 caataacgct tgtccatccg gggtgtcgat ggtcgccgta acgaccggaa cactacccgg
     2401 acgtcgcaag gcttccgcca cgtcacgcat ttcatttgga ttttcttgaa ccatgcgacg
     2461 aaacgcatcc agcggcatct ctcgcttcgc ttcatcgctc agcatcgcat aagcgtcctc
     2521 gatacggttt tggtcgagag cgtccgcata gccgcgtaac gtttccgccg gaccacgcac
     2581 tggtgtgccg cggactccgc agccgccgac catggtgagg accacgaacg gcaacaggta
     2641 ccgaaacgtg ttctttttgc ctggcgcccg atgggtgtcg ctttgcctca tggtgatatc
     2701 gttgcacatg ggatcgattc gtcgcccaaa ccgttcaagg atgagtttcc gaagggcaga
     2761 gtatacgagg ccagcccggc aagcacgact gatcgttacg gaacaaggtc ctttaggggt
     2821 tggctcgatg tgatttcccg ctcccccttt ggctttgttc ccgggccgta ttacggttcg
     2881 gacatgagtc gcggaccgtc agccgcccag gaaggcatca gcatccccat tcttcccctt
     2941 cgcaactcgg tactatttcc catgtccgtg gtgccgatca atgtgggaag gccaagaagc
     3001 gttcgcttga tcgaagacgc cctcggcatg gagcgtgcca ccatcggcat attgacccag
     3061 tttgcgtccg ataccgatga gccgacgttt cgtgacctct acaaaatcgg cacgctcgct
     3121 cgaatcgttc aagtcattcg tgtcggtccg tccgtatata cggtgaagat cagtggtatc
     3181 gcacgttttc gcgtgttgag cggacaagga ctcgaaccgt tcatgcgcgc ccaggtgcaa
     3241 cgcatcatcg atccggtaga ccacgacgag caatcggatc aactcagtgc gcaccttcgg
     3301 gaaagcgcaa gaaccctgct gacgcgcaac ccggccctgc cccaggaact caacggtatt
     3361 ctcgaaaacg tcagggaacc cggtgctctc gcggacctgt tggtagccag ctttcccgcc
     3421 gaattcgctc ctgtcgacga gcgccagcaa gtgctcgaaa ccttcgacat caaaaaacgc
     3481 atgatgctcg tgcaggaaat gatcgatcgg caactcaaag tgctgaaggt t
//
LOCUS       DAOSOR010000599         3523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_26691_length_3523_cov_3.767301, whole genome shotgun sequence.
ACCESSION   DAOSOR010000599 DAOSOR010000000
VERSION     DAOSOR010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26691_length_3523_cov_3.767301"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            757..1314
                     /locus_tag="PK710_22265"
     CDS             757..1314
                     /locus_tag="PK710_22265"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOT12521.1"
                     /translation="MTPAVFTPATDLPTLDDVSLLWDRFGMLDHIRDHSRVVGHVALM
                     LTTWLAQVGILLDPRAVEVGALLHDIAKTPCLGTTRRHDREGENILRDLGYPEVAYLI
                     GVHVRLPQPHPIDESFVVYYADKRVMHDRIVDLGQRFSYIAERYGKGDAESLKRIEVA
                     RLRAFAAEQELFALLPSHRPEDIQA"
     gene            1311..2510
                     /locus_tag="PK710_22270"
     CDS             1311..2510
                     /locus_tag="PK710_22270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12522.1"
                     /translation="MKPWYGRFRALWRRLRLSKRIAIFVGLGVLLLSVSKTACYAFFR
                     PSHLDRSHPAVREDLAARRRYLIHAVTDLSSGKMPWYLPDQFKGEWLAVTYSMTAMAL
                     VNLSRIYPDTVGESAQILDQMAAAMETDDVTSFDRALWNEDPLSSLDGPHGHIGYLGH
                     LAIVLVAKKYVNPASPNDQRLRSILLALHRRMLDSPWILAETYPGQIFVADNAVVVAS
                     LSLAQPLFPDVSLDISERWVRSIQRRYLDPDTGLMVFRVGTDGSVIEKSRGSGVAWSI
                     FYLSYAHDSFARQQYQQLRSTLLSQPFGLFHGIREVPVGNDWQGDVDSGPVLFGLSPS
                     ATGFAIAGAVLTGDDDTTLHLLRTAEWAGTTVSTGQGRRYLLAPLVGDAILLAMRTVT
                     PWRQPVP"
     gene            2613..3026
                     /locus_tag="PK710_22275"
     CDS             2613..3026
                     /locus_tag="PK710_22275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12523.1"
                     /translation="MADVSTSGKTHLVANLVLQILMLVGHLMLGIGAAVSAVLFFYGD
                     AGWGPFATDAADAGRRTGNLLAIIALALWSCVGLVWTPLNAFGLWNRRPWARSSTSAY
                     WLLSALTICLLPLSVYGLLSLARTDVREALEPDSN"
BASE COUNT          701 a          945 c         1017 g          860 t
ORIGIN      
        1 ttccaagcct cttcccattg aggtgctgtc tgaccgggta atggatcata cccgaatcga
       61 aggctgatca accatgcaac ggcccgcatt cttcgatttt ttgtgtagat agcccgtgat
      121 cgtcgttctc ttcgagcgag caatcgacgt tgccaagtcg gatgacgtcc cccaacctta
      181 tcgggactgg ttccgaacca actgggtttt cattcacgaa cgtaccgttt cgactcccaa
      241 aatcgtggat gaaaagctct cgccctcggg tgaggatgca agcatgcaga cgtgatatgg
      301 aagaatgggc aatgacgatc gagcaatagt cgctgcgtcc aatcacgcat tcttctccgc
      361 gtaacggtgc cctcgtatga aaaaaacgaa gctggagcga gcgggtcgtc atgcgatagc
      421 ctattgtgaa acggctggcg tcacaggcca agatgcatcg tcgtgaaacg aattcgctgt
      481 gggtacgatt ggggcaataa gaagaatctt ctgtaaatta ggaaatttgc aatctctcat
      541 gagttcatgg tttttatttg ttgcttcgta cgcgggggtt gaagtcattt ctctgtaatt
      601 atacgtattt atgcgacttt tgccgcagtg gttcaggagc gcctcatttt tcgcgggcag
      661 cggtttgccc cggcgcaggc gaagccaacc gtcggctccg ttgatcccga ccgacatccg
      721 gcgcaaaatc atagccatga tcgccaggca cttttcatga cgcccgccgt attcaccccc
      781 gccacagatc ttcccaccct ggatgacgtc tcgctgcttt gggaccgatt cggaatgctc
      841 gaccatattc gagaccatag ccgggtcgtg gggcatgtcg ctctcatgct gacgacttgg
      901 cttgcgcaag tgggcatctt gctcgacccg cgcgccgtcg aagtcggtgc tttgctgcac
      961 gatatcgcaa aaaccccatg cttgggcaca acgcgtcgcc acgatcggga aggagagaac
     1021 atcctgcgtg acttgggata tccggaagtt gcttacttga ttggtgtgca tgtgcggttg
     1081 ccacaacccc atcccatcga tgaatccttc gtggtgtact atgccgacaa acgcgtgatg
     1141 cacgatcgga ttgtggactt gggccaacgc ttctcttata tcgccgaacg ctatggcaaa
     1201 ggcgatgcgg agtcgttgaa acgcatcgaa gtagcgcggt tgcgtgcatt cgccgcggaa
     1261 caagaattgt ttgctcttct gccctctcat cgacccgagg acatccaagc gtgaaaccct
     1321 ggtatggccg atttcgggcc ctgtggcgac ggcttcgact gtcgaaacgc atcgcgatct
     1381 tcgtaggtct cggcgtgctg ctgctgtcgg tatcgaaaac ggcatgctac gcgtttttcc
     1441 gtccatccca cctcgaccga tcgcaccccg ctgtacggga agacctcgcc gcgcgcaggc
     1501 gatacctcat ccacgccgtt accgacctgt cgtcgggcaa aatgccctgg tacctgcccg
     1561 atcagttcaa aggcgaatgg ctggccgtta cctattccat gaccgcgatg gcgctggtga
     1621 atttgtctcg gatctatccg gatacggtcg gtgaatccgc gcaaattctc gatcagatgg
     1681 ctgccgcaat ggagaccgac gacgtaacat ctttcgatcg ggccttgtgg aacgaggacc
     1741 ctctttcgtc gttggatggc ccccatgggc atattggcta cctcggccat ctggcgatcg
     1801 tgctcgtcgc aaaaaaatac gtcaatccgg cttcccccaa cgaccagcga ctgcgctcta
     1861 tcctgctcgc gttgcatcgt cgtatgctcg acagcccgtg gattttggcg gaaacgtatc
     1921 cagggcaaat atttgtggcg gataatgccg tggtggtggc cagcctttcg cttgcacaac
     1981 cgctgtttcc ggacgtttcc ttggatattt ccgagcgttg ggttcgctcg atacaacggc
     2041 gctatttgga tccggatacg ggattgatgg tgtttcgggt gggaaccgat ggcagcgtga
     2101 tcgagaaaag tcgcggctca ggagtcgcct ggagtatctt ctacctgagt tacgcccatg
     2161 attccttcgc acgacaacag taccagcagt tgcgctcgac gttgctcagc caacctttcg
     2221 ggttgtttca tggcatacgg gaagttccgg tcggcaacga ttggcagggc gatgtggatt
     2281 caggccccgt gctgttcgga ttgagccctt cggccaccgg ttttgccatc gccggcgcgg
     2341 tgctcacggg tgatgacgat accaccttgc acttgttacg gactgccgaa tgggcaggga
     2401 cgaccgtgag tacggggcag gggcgacgat acctgctcgc gccgttggtc ggagatgcca
     2461 ttttgcttgc catgaggacg gtgacaccgt ggcggcagcc cgtaccgtaa ccaatctacg
     2521 cttccaggac ccaggaggcg tcgcaggcgg cggcgcgggt ttgatgttgc cttgggttcg
     2581 accgacagac catgctaggt ctcggtgggt acatggctga cgtatcgact tcgggaaaaa
     2641 ctcaccttgt agcgaatctg gtgttgcaga ttctcatgct tgtgggtcat ttgatgctgg
     2701 ggattggcgc ggcggtcagc gccgttttgt ttttttatgg ggatgccgga tggggcccct
     2761 ttgccacgga tgccgccgat gcagggcgac gcacaggcaa cttgcttgcg atcatcgcgc
     2821 tggccctgtg gtcttgcgtg ggattggtct ggaccccttt gaatgccttt ggactttgga
     2881 accgtcgccc ttgggcgcgc agcagcactt ccgcctattg gctcctgtcc gcgcttacca
     2941 tctgcctcct accgctatcc gtatatggac tgctcagctt ggcacgaacg gacgttcgcg
     3001 aggccttgga gcctgattcc aattgaatct attaaattgc catatttgaa gttgttaaaa
     3061 taataaatct agaaaacaag gatgggtcgt tggtggcgga cgcttttgct tcagccagcg
     3121 ggccaaacct ttttcatccc acttcgcggg ccaatcgaat ccagaggttt ttcggtggcg
     3181 acctccgacc gcctctgata atcttcgaaa attgacgaat acgaacatct gaccgcgcat
     3241 ggaggagcca tgaggagtca tgagtaagat gcttcatgac atctctttat gccagtgtct
     3301 ggagcctcat agggttcgtc tttgtctcga aggtcgttcg gcaatggtgg atttgggctt
     3361 gacgggtgaa cagagcgagt ggctggcacg tggcgacaag aggccaattc gagagcattt
     3421 tcgagggagc ctatgacgca tccattcgct ccgggacaga tcttggagga taaataccgg
     3481 atcgatagtg tcatcggcga aggggggatg ggcgtggtgg ttg
//
LOCUS       DAOSOR010000600         3522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_26706_length_3522_cov_3.549178, whole genome shotgun sequence.
ACCESSION   DAOSOR010000600 DAOSOR010000000
VERSION     DAOSOR010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26706_length_3522_cov_3.549178"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..1625
                     /locus_tag="PK710_22280"
     CDS             183..1625
                     /locus_tag="PK710_22280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT12524.1"
                     /translation="MSQGTEDKKALGRIALQQRTVVSRGAAEEEPVDLEGLKALSEQH
                     GVPGIDLTQVCIRLQDLDLLPREIAKRHGILPVLVRDDRVFVAMVSPGDKHVIDELEF
                     VTGKRVYPYVALPSALSKAQEAAYEQRNRGEELYIGPRCPEETLRRMGVLAQSEGEMP
                     FDATPAPASEPQEPPRVVGDVTDRAASDSELSEADFPNTSVDLSVVTDLPDSAPMAGR
                     SAAESDVRTILIVDDEVDIRKMLARLLTDRGYQVLEADRGLQALRLVKEKVPDIIILD
                     AMLPEVHGFEIARRIKGSQRYGHIPIIMISAVYRGWRYAEDVKASYGVNAYIEKPFRI
                     AEILKAIDEVAKTSSSDKPAIAERKKVSEAAEQALAAGVAAYQAGNLDAAIEHLKQGV
                     NIDPLAYRLHFHLGLLCGKKGLVYEAIQHLERALDINARHFPAMKNLAVLYQKAGFRN
                     KAIETWERAIAIAPDEATKQSIKELLLGLL"
     gene            1682..3355
                     /locus_tag="PK710_22285"
     CDS             1682..3355
                     /locus_tag="PK710_22285"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HOT12525.1"
                     /translation="MRFLCPKCKAKYQIADDKLEGRAVRMKCRKCGDIIDVPSPTELS
                     AHPPTSPPPKPPAASGIQKKAAPNLKPPAKPARPLGIAPRPSTALGKPPQPSSKTPQP
                     PVAAKPEPPKPAVASTAPEVPSEPSVGGLADAFAQRVREPQTSDVSAAIEVLSAGAGE
                     EWYVGINGVPLGPVRLSTLRQKAQQGAIDENSLVWRAGFEEWLPLRTFPELLVLVREG
                     QESGSRASLLPAVPQPSVPARTAFSPGAFGHMAAPTHSSAPPTVSESAAPSDPDLDSP
                     TIIASGVQPSGVAILTTDPFASTQPAPEPSASPSVPAAARKSFVDDLTLGVRKQVRMH
                     PAAYVLIAIAFGFGATGAIVFFTNDREPAPPPTIQVVTVTAPPTAPSELDASAAASVA
                     MNTDSLPTEVKGGSVPRAGSESKTPNGKEPSKTDETTGVPRLGLGPSPLVDGPSVGGP
                     RSEGGGNLPQQLDQSDIERVVNAHRSSVKRGCWEPALAGRSPNAPKSAKVTVLISIGP
                     SGQVTQASASGGDGFPGLSSCIVSKVRAWKFPPSGGTSQANVPFAFFSQ"
BASE COUNT          762 a         1006 c          989 g          765 t
ORIGIN      
        1 aaggaagatt cgagaatttt tttcaaatat ttctggatcg acacaaagaa gaattaaaaa
       61 gcgcgtgaaa actatcgcca tttttttctc gtgggatgtc aaactgttgt cggagcccta
      121 tccgttggat atcgtgggct tcgaatgagg tgtcgctaca tgaacggcat tcgagtgtcg
      181 ccatgagcca aggtaccgag gataaaaagg cgcttggtcg cattgccttg caacagcgga
      241 ccgtggtttc caggggtgct gcagaggagg agccggttga tctggaaggg ctcaaagctc
      301 tttcggagca gcatggtgtg ccggggatcg atctgacgca ggtttgtatt cgtttgcagg
      361 atttggattt gctgcctcgg gaaatcgcaa aacgacacgg tattcttcct gttctcgttc
      421 gtgacgatcg cgtgtttgtg gcgatggttt cgccggggga caagcatgtc atcgatgagt
      481 tggagtttgt gacgggcaag cgtgtgtatc cttacgtagc tcttccaagc gcattatcga
      541 aggctcagga ggcggcgtac gagcagcgca atcgtgggga ggaactctat atcggtccgc
      601 gttgtccaga agaaacgttg aggcgtatgg gggtgttggc gcagtcggag ggagaaatgc
      661 ccttcgacgc tacacctgcg cctgcttccg aaccgcagga gccgccgcgc gtcgtgggtg
      721 atgtcacgga cagggccgct tccgattcgg agctttcgga agccgatttt ccgaatactt
      781 ccgtggatct ttcggtggtt acggacttac cggatagtgc gcccatggcg gggcgttcgg
      841 ccgctgagtc tgatgttcgc acgattttga tcgtggatga cgaagtggac atccgaaaga
      901 tgctggctcg attgttgacc gatcgaggct atcaggtgct cgaagccgat cgaggcttgc
      961 aagccttgcg gttggtcaag gaaaaagtgc cggatatcat catcctcgac gccatgctgc
     1021 cagaagtgca tggcttcgaa atcgctcgtc gaatcaaggg aagtcagcgc tacggccata
     1081 ttccgatcat catgatctcg gcggtctatc gtggatggcg ctatgccgag gatgtcaagg
     1141 cgagctatgg cgtgaatgcc tacatcgaaa aaccttttcg aatcgctgaa attctcaaag
     1201 cgatcgacga agtggcaaag acctcatcgt cggacaaacc ggcgatcgca gagcgaaaaa
     1261 aagtatcgga agccgcggag caagcccttg ccgcgggggt agccgcgtat caggccggaa
     1321 acttggatgc ggcgatcgag catctcaagc agggagtgaa catcgacccg ctggcttatc
     1381 gtcttcactt tcaccttggc ctactttgtg ggaaaaaggg attggtatat gaggccatcc
     1441 agcatctcga acgcgcgttg gatatcaatg cccgtcattt tccagccatg aaaaacttgg
     1501 ctgtccttta tcagaaagct gggtttcgca acaaagccat tgaaacctgg gaacgagcca
     1561 tcgcgattgc tccggatgag gccacgaaac aatcgatcaa agaactactt ctggggttgc
     1621 tctaaacgac tggcatcctc aggatcgctt caacgatgat gtcgcccgag tatagtcttc
     1681 catgagattc ctctgcccga aatgcaaggc gaagtatcaa atcgccgacg acaagctcga
     1741 gggccgagca gttcgcatga agtgccgaaa atgcggcgat atcatcgatg tgccgtcgcc
     1801 caccgaactt tcggcccacc cgcccacctc cccaccgccc aagccacccg ccgcttccgg
     1861 gattcaaaag aaggctgctc caaacctgaa gccccctgcc aagccggcgc gcccgttggg
     1921 gattgctcca agaccttcga ccgcgctggg caagccaccc caaccgtctt cgaaaacgcc
     1981 acaaccaccg gttgctgcta agccagaacc tcccaagcct gccgtcgctt ccactgcccc
     2041 agaagtgcca agcgagccat cggtgggtgg attggccgat gctttcgcac agcgagtccg
     2101 agagcctcaa acgagcgatg tctccgccgc catcgaggtg ctttccgcag gcgccggtga
     2161 ggaatggtat gtcgggatca acggcgtgcc ccttgggccg gtgcgcctct cgaccctcag
     2221 gcaaaaggcc caacagggcg ccatcgatga gaactccctc gtatggcgag cgggatttga
     2281 agagtggttg ccgcttcgga cgtttcccga acttctcgtt cttgtgaggg aaggtcaaga
     2341 aagtgggtca agagcctcac tgctacccgc tgttccacag ccgtcggtgc ctgctcgtac
     2401 cgctttttct cccggtgcct tcggacatat ggcggctcca acgcattctt ccgcaccgcc
     2461 caccgtctcc gaatccgctg caccaagtga cccggatctg gattctccca ccatcattgc
     2521 ctccggtgtc caaccttccg gcgtagccat tctcaccact gaccctttcg cttccaccca
     2581 acctgccccg gaaccgtccg catccccatc cgtacctgcc gccgcgcgaa agtcgttcgt
     2641 cgacgatctc acgttgggag tccgcaagca agtgcgtatg caccctgccg cttacgtgct
     2701 cattgccatt gctttcggat tcggagcgac aggggccatc gtatttttta ccaatgaccg
     2761 ggagcctgca ccaccgccga cgatccaagt ggttacggtc acggcgcctc ccaccgctcc
     2821 ttcggaactg gatgcatctg ctgccgcgag tgtggctatg aacacagact cgcttcctac
     2881 cgaagtgaag ggtggatccg ttccccgagc gggatccgaa tccaaaactc caaacggaaa
     2941 agaaccatcc aaaaccgacg agacgaccgg tgtccctcgt ttggggcttg gtccttcgcc
     3001 gcttgtcgat gggccttccg tcggaggacc gagatccgaa gggggaggaa accttccgca
     3061 acaactcgac caatccgata tcgagcgtgt cgtcaatgct catcgatctt cggtcaaacg
     3121 cggatgttgg gagcccgcgc tcgccggacg ctctccgaac gctccgaagt ctgccaaagt
     3181 aacggtcttg atctcgattg gaccaagcgg tcaggtaacc caagccagcg cttctggcgg
     3241 cgatggtttc ccagggcttt caagttgcat cgttagcaag gtgagagcat ggaagtttcc
     3301 gccttcggga ggtacttctc aggccaacgt gcccttcgcc tttttctcac aataggcgta
     3361 tctctgtcag tggtcttttt ggatagccgc gatccaaaag ccgccaaaga tggatagacg
     3421 gctgtcacat atcgcccact ccaatcgtct aagaccgtga tttaacagcg gaatattcat
     3481 tgccgtcgcg acgggaatcg cgatgcggac accgcgcgaa tc
//
LOCUS       DAOSOR010000601         3520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_26721_length_3520_cov_3.546320, whole genome shotgun sequence.
ACCESSION   DAOSOR010000601 DAOSOR010000000
VERSION     DAOSOR010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26721_length_3520_cov_3.546320"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /locus_tag="PK710_22290"
     CDS             <1..1295
                     /locus_tag="PK710_22290"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOT12526.1"
                     /translation="CDGCIEALRQHEIVVGDRLILQGKEQSLRRLEQLGLISELHWLD
                     RKEAAELAGGCAELLPLRVPEGSVLAGSNLLDSRMGHGFGLTAVGLIRGGEARCMPSP
                     TERIEAGDLLILQGSSRDFEVLDALQALEIREPGKAEVAELESQQVGVTEVLLSPRTS
                     LAGKTLGALMFRERYGVSVLAIWREGRAFRTGLQEKALQFGDALLVYGRRSDLALMAR
                     DPDFVVLDEAAAQAPRLDKAGLATAIMLGVLASAMLGYLPISIAALMGAALMVLVGCL
                     RMEEAYQAIEWKVVFLIASMLPLGAAIESTGVSQIAAEMLIAGVGKYGPRSVIAALFA
                     VTVIGVQVIPSAAIVVLMAPIALSTSSTLGISSHLLMMTVSMAAAASYASPLSHPAHL
                     LVMGPGGYRFMDYVKVGVPVTLLAFGVSVVLLPLMWPP"
     gene            1377..2849
                     /locus_tag="PK710_22295"
     CDS             1377..2849
                     /locus_tag="PK710_22295"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT12527.1"
                     /translation="MTIPPEAANLYPEPGTVIDNKYRCERLIGAGGMGAVALATHLIR
                     KAPVALKFMHPGVSIQEGFVERFINEAVAASRIDSEHVVKVFDVGQLQEGTPYLVMEY
                     LEGIDLADVLERDGAPGLGSIPRCLHFAVQILRGLQTAHDAGIVHRDMKPSNCFVIEK
                     DGDPDFVKLLDFGISKLQQPGDVKLTQTNSLLGTPLYMSPEQSRSAKLADHRTDLYAV
                     GVIIYELLTGKPPFDSDSTFTELLLKIATEEPIPLREFRPDVPEELAAVIRRAFARNP
                     NDRYGSAYELSEALAPWCDARSLPILEGARRRAARITGLPHTMLSSGTTQSSETAPAA
                     TGYTGVSAPEKTQAVVPASLVTSQAGVAAPAMQGRARSPSRMAFALVGVGAMVLAAAA
                     LWFFSRPSAQDTPPASLAVSVVEHTTPPPPTTVTASSAPIVVVAQETPSSIPSASASV
                     QVTLPIRTTSTAKATTPPPPPPSPEGTSTKPKIGDITIQH"
     gene            2920..>3520
                     /locus_tag="PK710_22300"
     CDS             2920..>3520
                     /locus_tag="PK710_22300"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOT12528.1"
                     /translation="MMVSVLAMLASSVPSVVHAQAKPTKSQARPSIRAELPEEARRSW
                     DLAIELFEARDYQGARVEFMRAFDLSRNPRVLFNVGVCDKNLRRYARAAAVWRQQLDT
                     GSAQLSEEDISTTRAAIAAVEPFVGMLTVRANIEGATLLIDGEEAGQTPFLGPISVDV
                     GRHEVRLRKPDYRDHIEEVEIASGQPGLIDIKLDPVVKTA"
BASE COUNT          692 a          858 c         1197 g          773 t
ORIGIN      
        1 ggtgtgatgg ttgtatcgag gcgcttcgtc agcacgagat agtggtgggg gatcggttga
       61 tactgcaggg caaggagcaa tccttgcggc gtctggagca attgggattg atttctgagc
      121 ttcattggct ggatcgcaaa gaggcggcgg aattggcggg tgggtgtgcg gagttgttgc
      181 cgttgcgtgt gccggaaggt tctgtgctag cggggagcaa tttgttggac agtcggatgg
      241 gccacgggtt tggattgaca gcggtaggtt tgattcgtgg gggggaggcg cggtgtatgc
      301 catcgccgac ggagcggatc gaggcggggg atcttctgat attgcaaggg tcatctcggg
      361 attttgaggt attggatgct cttcaagctc tcgagattcg ggagccgggc aaggcggagg
      421 tagctgagct ggaatctcag caggttgggg taacggaggt gttattgtct ccgcgcacgt
      481 cacttgcggg aaaaacgctt ggggcgctga tgtttcgtga gcgatatggt gtgagcgtgc
      541 ttgcgatttg gcgagagggc agggcgtttc gcacggggct ccaggaaaaa gcgttacaat
      601 tcggtgatgc gctgctcgtc tacggtcgac gcagtgacct agccttgatg gcgcgagatc
      661 cagattttgt ggttttggac gaagcggcgg cgcaagcacc gcggttggac aaggcggggc
      721 tggcgacagc gatcatgctg ggggtgttgg cgagtgcgat gcttggatat ttgccgattt
      781 ccatcgcggc attgatgggg gcagcgttga tggtcttggt gggttgtttg cgtatggagg
      841 aggcatatca ggcgatcgaa tggaaggtgg tatttttgat tgcgagcatg cttcccttgg
      901 gggcggccat cgagtccacg ggggtttcgc agattgcggc ggaaatgctc attgcgggag
      961 ttgggaagta cggtccccgc tcggtcatcg cggccttatt tgcggtgacg gtgataggcg
     1021 tgcaagtcat cccatcggcg gccattgtcg tgttgatggc ccccattgcg ctgagcacgt
     1081 cgagtacgct gggcatatca tcgcatttgc tgatgatgac ggtgtcgatg gcagcggcgg
     1141 cgagttatgc gagtccgttg tctcacccgg cgcatctttt ggtgatgggt cctggtgggt
     1201 atcgtttcat ggattacgtc aaagtgggag ttccggtaac gcttctggcg tttggggtat
     1261 cggtggtgct gcttcctttg atgtggccgc cgtgacagaa tcgcaaaggc ttgccgacac
     1321 agcctcatgg tgcaaggtat actcaacggg ccaccgcacg cctgcgagaa aaagccatga
     1381 ccattccgcc cgaggccgcc aatctttatc ctgaaccagg tacggtcatc gataacaagt
     1441 atcgatgtga acggctcatt ggcgcgggcg gcatgggggc ggtggcgttg gctacgcatt
     1501 tgatacgcaa ggccccggta gcgttgaaat tcatgcatcc gggcgtatcg atacaagaag
     1561 gattcgtcga acgattcatc aacgaagcgg tggcggcatc gcgcatcgac agtgaacacg
     1621 tggtgaaggt gttcgacgtg gggcaattgc aagagggcac gccgtacctg gtgatggagt
     1681 acctcgaggg catcgatttg gcggatgtgt tggagcggga tggcgcaccg gggctgggca
     1741 gtattcctcg gtgtttgcac tttgcggtgc aaatactgcg tggattgcag accgcccacg
     1801 acgctgggat cgtgcatcgg gacatgaagc catcgaattg tttcgtcatt gaaaaagatg
     1861 gcgatccgga tttcgtcaag ctgctggact ttggcattag caagcttcag caacctggcg
     1921 acgtcaaatt gacgcagacg aactcgctct tgggcacgcc gctgtatatg tcgccggagc
     1981 agtcccgtag cgcgaagtta gcggatcatc ggactgattt gtacgcggtg ggggtcatca
     2041 tatacgagtt attgacgggg aaaccgccgt ttgactcgga ttcgactttc acggagttgt
     2101 tgctcaagat cgcgacggaa gagccgattc cgttgcggga attccgaccg gatgtacccg
     2161 aagaattggc cgcggtcatt cgtcgtgctt tcgcgcgaaa ccccaacgat cgatacggct
     2221 cggcgtacga actcagcgaa gcgcttgcgc catggtgcga cgcgaggagc ttgccgatcc
     2281 tggagggagc ccgtcgacga gcggctcgaa tcacgggttt gcctcatacg atgctttctt
     2341 cgggtacgac ccaatcgtcg gaaaccgcac ccgcggcgac ggggtatacg ggcgtttccg
     2401 cacctgagaa gacacaagcc gtggtgcctg cttcgctggt gacgtcacag gctggggttg
     2461 cggcgccggc gatgcaaggc agggcacgtt cgccatcacg tatggcattt gcgttggtcg
     2521 gcgtgggagc catggttctg gcagcggcgg cgctatggtt tttttccagg ccgagcgcgc
     2581 aggacacgcc ccctgcatcg cttgcggtgt ccgtcgtaga acacacaaca ccgccgccac
     2641 cgaccacggt tacggcatcg agcgctccga tagtggttgt ggcgcaagaa acgccgtcgt
     2701 cgattccttc tgcttccgcg tcggtgcaag tcactctacc gattcgaacg acttccacgg
     2761 caaaggcaac gacgccgcca ccaccaccac catcacccga gggaacgtct accaaaccga
     2821 aaatcggaga catcacgatt caacattgag gcgatgcaat cagatgacaa ttcgggcaat
     2881 gcaagaagag aaaggggcac gacgatgagg ggcgcgatca tgatggtatc ggttctggcg
     2941 atgctggcat cgtcggttcc ctccgtggtg catgcacagg cgaagccaac caagtcgcaa
     3001 gcacggccat cgattcgcgc cgagcttcca gaagaagcgc gtcgttcgtg ggatttggct
     3061 atcgagctat ttgaggcacg ggattaccaa ggggcgcggg tcgagttcat gcgagccttc
     3121 gacttatcgc gcaacccgag ggtgttgttc aacgtaggcg tatgcgacaa aaacctacgt
     3181 cgttatgctc gtgcggccgc ggtgtggcga cagcagttgg acacgggtag cgcgcaattg
     3241 tccgaagaag atatcagcac cacgcgagct gccattgcgg cggtagaacc attcgtgggg
     3301 atgctaacgg tgcgggccaa tatcgaaggg gcaacgctgc tcattgatgg ggaggaggcg
     3361 ggtcaaacac cgtttcttgg gccgatttcc gtcgacgtgg ggcgtcatga ggttcgactt
     3421 cgcaagccgg actaccgcga ccacatcgag gaggtagaga ttgcgagtgg gcagccgggg
     3481 ctcattgata tcaagctcga tccggtggtc aagacggcgt
//
LOCUS       DAOSOR010000602         3516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_26761_length_3516_cov_3.704421, whole genome shotgun sequence.
ACCESSION   DAOSOR010000602 DAOSOR010000000
VERSION     DAOSOR010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26761_length_3516_cov_3.704421"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="PK710_22305"
     CDS             complement(<1..927)
                     /locus_tag="PK710_22305"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOT12529.1"
                     /translation="MRARWLFGPILILACALSWACSGPPESMGKRSRKRYEAFCQAHV
                     LGLGTIDVESDYLPHVVQCENGGAPSASLQAQAVAARSYLYYKLETTGLIRDGEMDQV
                     YTCPGAPTREHVQSVLQTAGQILTYKGSTLCAFFVAGAKQSPPACRGNIEHDTERYVT
                     INWGVSGTEVRQSPLGLVHPSNHQNRGCLSQWGSRCLATMGWSYLDILKFYYGADIVL
                     EKASGPCVGDSNRPPVGRVVHIDCEAITGWVQDPDEPEVALRVHGFFGGSTGSSQAIQ
                     VVSVSTTPPRCDSDPPCPKAFSIPIPYRLRDGK"
     gene            1033..1266
                     /locus_tag="PK710_22310"
     CDS             1033..1266
                     /locus_tag="PK710_22310"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOT12530.1"
                     /translation="MNVQIHFHGAIDRRGFEQDHIVTCPQGTTVEQVLELLAYPPLQL
                     RAIVAVVKGRRVDRNEPLQDGDTLDLMVPAGGG"
     gene            complement(1328..2569)
                     /locus_tag="PK710_22315"
     CDS             complement(1328..2569)
                     /locus_tag="PK710_22315"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOT12531.1"
                     /translation="MRVWGFSFFFFLGCAPSYQTDPLPVPVTPTPIEVQQDPFPSPEQ
                     PISQEPPPAPALEPCKGHAYLSNVPFLKGQLHVHSDRSFDAATPVEDVIAFYAKRGYD
                     FISITDHNHVTVPKEHPSGLLTISGVELTYNAATCDPPPRPGYLCAFHAGVLFLNPMR
                     DVTRGRHFNLPFRRQRLEVWQNLLARAEDLDGVFVLNHPTFHYAIDETQLDELVGRGV
                     RLVEFFNAGVIDHDKAGPTVAIQQAEALWTAMLDRGHPVIAVGGDDAHHFADAATIRM
                     TGKKPLLGDRVWVMVRAQRSVEAIREAFIQGDFYVSTGVRLSELEVSDLNVHLRIDPR
                     EGQSYSTTFVGQGGRILAQVDGTEACFPTEKAEGYVRAVVQSDDGEMAWTQAVMLESN
                     DARPSPGPIAKATGNMGLSTQ"
     gene            2717..>3516
                     /locus_tag="PK710_22320"
     CDS             2717..>3516
                     /locus_tag="PK710_22320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12532.1"
                     /translation="MPSSVHFFPLIVCLALVGCGKKAPNPEGNEYSSSDPASRRDACI
                     HTDGCRDHGRCTEQQARCVVATDRDCHQSRDCVRDGLCHVVGDGCQATSDEDCRATRR
                     CRENGLCSARNGICVAASVRDCLSSTECKRRGRCDMVNGACEATTDASCKSSILCKEK
                     GYCGIEAGECKAKTDDDCASSADCIDYGQCKFVQGACVAVDDGMCRRSKRCRYLGLCS
                     AREGQCIAASDQDCRSQQACELHGRCTAKQGRCEVGSDDDCRWSEVCHR"
BASE COUNT          774 a          913 c         1102 g          727 t
ORIGIN      
        1 tttcccatca cgcagacggt agggaatggg tatggaaaac gctttggggc agggagggtc
       61 ggaatcgcat cggggcggag tggtgcttac ggacacgacc tgaatggcct gggaggaacc
      121 tgtagaaccg ccaaaaaagc catgcacgcg caaggcaact tccggttcat ccgggtcttg
      181 tacccatccg gtgatagctt cgcagtcgat gtgaacgacg cgcccgacag ggggacggtt
      241 gctatcgccc acgcacgggc ccgaggcttt ttccagaacg atatccgcac cgtaataaaa
      301 tttgaggata tcgaggtagg accagcccat ggtagcaagg cagcgggagc cccattgcga
      361 caagcatccg cggttttggt gattcgaggg atgcacgagg ccgaggggag attgacgaac
      421 ttccgtgccg gaaacacccc aattgatagt cacgtagcgt tccgtatcgt gttcgatatt
      481 tcctcgacag gcggggggcg attgttttgc ccctgccaca aaaaaagcac ataaagtcga
      541 gcccttatac gtaagaatct gcccggcggt ctgcaacacg gattgcacgt gttcgcgagt
      601 cggtgcccca ggacaggtat agacttggtc catttcccca tcgcggatga ggccggtggt
      661 ttcgagcttg taataaagat aggaacgagc ggccaccgcc tgggcttgaa gggaagcgga
      721 aggcgcgccg ccattttcgc attgaaccac gtggggaaga tagtcggact cgacgtcgat
      781 ggtcccgagt ccgagaacat gagcttggca gaacgcttca taacgcttgc gggaacgttt
      841 gcccatggac tcgggaggtc cggaacaagc ccacgacaaa gcgcaagcca gaatcaagat
      901 aggtccaaaa agccaacggg cacgcacgac cctagtgtag ttccagacgc gatgcgtttg
      961 cgacagcgtt cttcccatca ggcgcacaac gcccctcgac gtgtttggca ttcatgctag
     1021 cgtcgttcgg agatgaacgt ccaaattcac tttcatggcg ctatcgatcg gcgcggtttc
     1081 gaacaagatc atatcgtcac gtgccctcaa ggaaccaccg tcgagcaggt gttggaactc
     1141 ctcgcctacc cgccgctcca acttcgagcg atcgtggccg tcgtgaaagg tcgaagggtg
     1201 gacagaaacg agccgttgca agatggagat acgcttgatt taatggtgcc cgccggtggt
     1261 ggttgacaga ccggagcaag acaacgcggt ttgatcccgc gtcgatcaag cggaagagga
     1321 cgcagcctca ttgcgtcgat aaacccatat ttcccgttgc tttggcgatc ggcccaggag
     1381 aggggcgggc atcgttggat tcgagcatca cggcttgtgt ccatgccatc tcaccatcgt
     1441 cgctttgcac cacggcacgc acatagcctt cggctttttc cgtgggaaaa catgcttccg
     1501 tgccatcgac ttgcgccagg atacggcccc cttgtccgac gaatgtcgtc gaatagcttt
     1561 ggccttctcg cgggtcgata cgaagatgca cattgaggtc ggagacttcc aattcgctga
     1621 ggcgaactcc cgtggacacg taaaagtccc cttggataaa cgcttcgcga atggcttcga
     1681 cggagcgttg tgcgcgaacc atcacccaga cgcgatcgcc gagcaagggt tttttcccgg
     1741 tcattcgtat cgtcgcggca tccgcgaaat ggtgtgcatc atcccctcct accgcgatga
     1801 ccggatgtcc acggtcgagc atggcggtcc acaaggcttc agcctgttgg atagccactg
     1861 tcggtcccgc cttgtcgtgg tcgatgactc cagcattgaa aaactcgacc aggcgaactc
     1921 cacggcccac caattcatcg agctgtgttt cgtcgatcgc ataatgaaac gtggggtgat
     1981 tgagcacgaa cactccatcc agatcctcgg cccgagccag gaggttttgc cacacttcca
     2041 gtcgttgacg gcgaaacgga aggttgaagt gccgtcctcg ggttacgtcc cgcatcggat
     2101 tgaggaacaa gacccccgcg tgaaacgcgc ataaataacc gggacgcggc ggagggtcgc
     2161 acgttgccgc attgtaggtt agttcgaccc cggaaatcgt cagcaagccg gaaggatgct
     2221 ctttgggaac ggtaacgtgg ttgtgatccg taatggaaat gaagtcatat ccgcgctttg
     2281 cataaaatgc gatcacatcc tcgacggggg tagcggcatc gaaagagcgg tcggaatgga
     2341 cgtgcagttg tcctttcagg aagggaacgt tggatagata agcatggcct ttgcagggtt
     2401 cgagggctgg ggcagggggc ggttcctggg agatgggttg ctccggtgat ggaaacgggt
     2461 cctgctgaac ttcgatcggt gtgggcgtca ccggaacggg cagaggatcc gtttggtagg
     2521 aaggagcaca accgagaaaa aagaagaaag aaaaacccca aactcgcatg ccgatacaga
     2581 catcctgcgc cttgcgcaag gtgatcgcaa gttaccggcg attcgaatgg aaaaccggag
     2641 ccgcgcaagg ttgtcgcgcg aggtcatgcg cagtaatgcg tgcttttctt gccgctgtct
     2701 tctacgatgc ccagccgtgc catcgtcggt tcatttcttt ccgctcatcg tttgccttgc
     2761 ccttgtgggg tgtggaaaga aagctccgaa tcccgaaggc aatgagtatt cgtcgtcgga
     2821 ccccgcctcg cgaagggacg cttgcatcca caccgatggt tgccgcgatc atgggcgatg
     2881 tacggaacag caggcgcgct gtgtggtggc caccgaccgg gattgccatc aatcccgcga
     2941 ttgcgtgcgt gatggtctct gccatgtggt gggtgatggt tgccaagcaa cctccgacga
     3001 agactgtcgg gcgacgcgtc gatgtcgtga aaacggtttg tgcagcgcac gaaacggcat
     3061 ttgtgtggcg gcatcggtga gggattgtct ttcctcgacg gagtgcaaga gacgtgggcg
     3121 atgtgacatg gtgaacggcg cgtgtgaagc cacgacggac gcttcgtgca agtcatcgat
     3181 tttgtgcaaa gaaaaggggt attgcgggat tgaagcgggg gagtgcaagg caaagacgga
     3241 cgacgattgc gcgagttcag cggactgtat tgattatggg caatgcaagt ttgtccaggg
     3301 agcctgtgtg gcggtggacg acggcatgtg tcggcggtcc aagcgatgtc ggtatcttgg
     3361 tctttgttcc gcccgagagg ggcagtgcat tgcggcgagc gatcaggact gtcgttctca
     3421 gcaagcgtgt gagttgcatg gccgatgcac ggccaagcaa gggcgttgtg aggtagggag
     3481 cgacgacgac tgtcgatggt cggaggtatg ccatcg
//
LOCUS       DAOSOR010000603         3515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_26774_length_3515_cov_4.121965, whole genome shotgun sequence.
ACCESSION   DAOSOR010000603 DAOSOR010000000
VERSION     DAOSOR010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26774_length_3515_cov_4.121965"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PK710_22325"
     CDS             <1..421
                     /locus_tag="PK710_22325"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOT12533.1"
                     /translation="QVASLSKKLEQMGKKVAFGIHPVAGRMPGHMNVLLAEADVDYDL
                     LKEMDEINESFASTDVVLVIGACDVVNPAAINMPDTPISGMPILNAHEAKRVLVCNLD
                     EKPGYSGVPNSLYEEETTLLLFGDANASVTELLGSLS"
     gene            446..1876
                     /locus_tag="PK710_22330"
     CDS             446..1876
                     /locus_tag="PK710_22330"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT12534.1"
                     /translation="MITIQQLSDKLLRAEYAVRGPIVIRAQELEAQGRKIIYCNIGNP
                     QALKQPPLTFMRQILSLVEYPELLTKAQELYPKDVVERARDILTKNPSGTGAYTQSAG
                     IPFIRKAVADFIANRDGIPANPANVILTDGASKGAQAVLTALLRSPKDGFMIPIPQYP
                     LYSASLTLYGGEQIGYYLDEENGWQLNETILTQSLNQAKSKGINPVGLVVINPGNPTG
                     AVLSQENIAMIIKFARQHKLALIADEVYQENVYATDREFHSFAKVMHALGDTDTPLFS
                     LHSVSKGFLGECGHRGGYLEMRNIPENVLAEFIKLQSISLCANVPGQLVTYLMVAPPK
                     PGDASHELYVRERDSILRDLKEKATLLGQGIQQVPGMFVDIPQGAMYAFVRFELPHKP
                     GVDPTQMSADQREKYEAERDNAYCLALLEETGICVVPGSGFGQRAGTLHFRTTFLPPR
                     DEMEDLVKHLGGFHVRYVEKLRSSAS"
     gene            complement(2036..2266)
                     /locus_tag="PK710_22335"
     CDS             complement(2036..2266)
                     /locus_tag="PK710_22335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12535.1"
                     /translation="MSMEPRLDRWMHLVHGADRLEESASEQAFEVACNYLQSVLEVFP
                     KDLDPVDDFEAYAVRRLARSILHVMKPPPPLP"
     gene            complement(2292..>3515)
                     /locus_tag="PK710_22340"
     CDS             complement(2292..>3515)
                     /locus_tag="PK710_22340"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HOT12536.1"
                     /translation="WLENPRAAETHSSESVARYMPATSTTEARKYHLQMPGYRMTPLK
                     GLTRLANMLGIGGIWVKDEAERFGLGSFKVLGGSFAIFRYIRDRLGNPTPFSHADLAR
                     DEVRKILKGLTFAAATDGNHGRGVAWAARQLGTESVIYVHERTSPSRIHAIEEAGARV
                     VVVPGTYDDAVRQVSKDATENHWQVISDTSWPGYEDIPAWVMQGYATLFLEVQQQMVA
                     LGDLRPTHVFVQAGVGSLAAAAAAFYANLYRNARPKIVVVEPERAACLYASLEANDGQ
                     PRSFPGDLDTIMAGLACGDPNPIAWQILRDTADIFLKCPDYVAGKGMRVLGMPLAGDP
                     TVISGESGAVTLGALMFVLRREEYRPLRERLGLNHNSQVLLVNTERNTDPDHFRQVVW
                     DGAASVPVAYRESYL"
BASE COUNT          815 a         1021 c          942 g          737 t
ORIGIN      
        1 ccaagtcgct tctctttcga aaaagctcga gcaaatgggc aaaaaagtcg ccttcgggat
       61 ccatccggtg gctggtcgta tgcccggaca catgaacgtg ctgctcgccg aagccgatgt
      121 cgactacgac ttgctgaaag aaatggacga aatcaacgag tctttcgcca gcaccgatgt
      181 cgtgctcgtc attggtgcct gcgacgtcgt caacccggct gccatcaaca tgcccgatac
      241 tcccatctct ggcatgccca tcctcaatgc gcatgaggcc aagcgcgtcc ttgtgtgcaa
      301 cctcgatgag aaaccgggct actcgggcgt tccaaactct ctttacgaag aggaaacgac
      361 ccttttgctt ttcggcgacg ccaatgcaag cgtcaccgaa ttgctcggaa gcctttcctg
      421 aggttgtgtg gatagcgagg tcatcatgat tacgatccag cagttgagcg acaaacttct
      481 tcgtgctgag tatgccgttc gtggaccgat cgtcattcgc gcccaggaac tcgaggccca
      541 gggccggaaa atcatttact gcaatatcgg gaaccctcag gccctcaagc agccgccgct
      601 cacgttcatg cggcaaatcc tgtctctcgt cgaataccct gaattactga cgaaagcaca
      661 ggagctatat cccaaggatg tcgtcgagcg agcgcgtgat attctgacaa aaaatccttc
      721 gggaaccggt gcttataccc aaagcgcggg tattcctttc attcggaaag cggtcgcgga
      781 cttcattgca aaccgcgacg gcatccccgc gaatccggcg aacgtgattt tgacggacgg
      841 ggcgagcaaa ggagcgcaag cggtgctcac ggcgctgctt cggtctccca aggatggatt
      901 catgattccc atcccccaat accctctgta tagcgcaagc ttgacgctct atggcggcga
      961 acagattggg tattatctcg atgaagagaa tgggtggcag ctcaatgaga ccattcttac
     1021 gcagagcttg aatcaggcga agagcaaggg aatcaacccg gtcggtctcg tggtgatcaa
     1081 tcccggcaac ccgacgggtg cagtcctgtc gcaagaaaac atcgccatga tcatcaagtt
     1141 cgcccggcag cacaaactcg cattgattgc cgacgaagtg taccaagaaa acgtatatgc
     1201 cacagaccgg gagttccact ccttcgccaa ggtcatgcat gcgcttggag atacggacac
     1261 gccgctgttc agcctgcact cggtttccaa gggatttctt ggggaatgcg gccatcgcgg
     1321 cggttacctc gaaatgcgca acatccccga aaacgttttg gcagagttca tcaaactaca
     1381 gtccatcagc ctgtgcgcca acgttcccgg acaactcgtt acttacctca tggtggctcc
     1441 ccccaagcca ggggatgcga gtcatgaatt gtacgttcgt gaacgcgact ccatcttgcg
     1501 cgatctcaaa gaaaaagcga ccttgctcgg ccagggtatt caacaagtcc ccggaatgtt
     1561 tgtcgatatt ccgcaagggg ccatgtatgc cttcgttcgt ttcgaattgc cacacaagcc
     1621 aggggttgac cccacccaga tgtcggcgga ccaacgggaa aagtacgaag cggaacgcga
     1681 caatgcctac tgcctcgctc tgttggaaga gaccggcatc tgtgtggtgc ccggatcagg
     1741 cttcggacaa cgggcaggaa cgctccactt ccggacgaca ttcttgccgc cccgcgacga
     1801 gatggaagac ctggtcaaac acctcggcgg cttccatgtt cgttacgtcg aaaaactccg
     1861 ctcctcagct tcttgatagc tgacaattcc taacacagcc tttgcagtca cctactgggc
     1921 acgtcggccg tacacgatcg accccttgcc cttgtgcccc atcatcgaag ctcaagcggc
     1981 agcgaagacg taagacattg atatttctac gtatttatac gatatggtcg agctatcagg
     2041 gaaggggagg gggtggtttc atcacgtgga gaatggagcg ggccagtcga cggaccgcat
     2101 acgcttcgaa gtcatcgacg ggatcgaggt ctttgggaaa cacttcgaga acgctttgca
     2161 agtagttgca cgcaacctcg aaggcttgct ccgatgcact ttcttccaag cgatctgcgc
     2221 catgaacgag gtgcatccac cgatcgagcc gaggctccat gctcatccag cgcctccaag
     2281 agatgaagat atcagaggta ggattcgcga taggcgacag gaacggaggc agcgccatcc
     2341 caaacgactt gacggaaatg gtcaggatcc gtgttccgtt ccgtattgac gagcagcacc
     2401 tgggaattgt ggtttagacc gagacgttcg cgaagcggac gatattcttc tcgacgcaac
     2461 acgaacatca gtgctccgag cgtaacggca ccggattcac cggaaatgac cgtcgggtcg
     2521 cccgcgagtg gcatgcccaa tacacgcatg cctttgcccg cgacatagtc cgggcacttg
     2581 aggaagatat ctgccgtatc gcgcaggatt tgccaggcga ttggattggg gtctccacaa
     2641 gccaaccctg ccatgatggt atcgagatcg cccggaaacg aacgtggttg gccatcgttg
     2701 gcttcgagcg aagcgtacaa gcaagccgct cgttcaggct cgactactac gatcttgggt
     2761 cgcgcatttc gataaagatt ggcataaaaa gctgccgctg ccgctgccaa ggagcctact
     2821 cccgcctgta cgaaaacatg cgtcggtcga aggtccccca gagccaccat ctgctgctgc
     2881 acctcgagaa aaagtgtggc gtagccctgc atgacccatg cggggatgtc ctcatagccc
     2941 ggccatgatg tatccgaaat cacctgccaa tgattctcgg tcgcatcttt actcacttgt
     3001 cgcacggcat cgtcgtaggt tcccggcacc accacgaccc gagcacccgc ttcctctatg
     3061 gcgtgaatgc gcgaaggcga ggtcctctca tgcacataga tgacggattc agtgccaagc
     3121 tgacgtgcgg cccaagccac gccacgccca tggttgccgt ccgtggccgc cgcgaacgtg
     3181 agccctttca agatctttct cacctcgtct cgcgcgagat cggcatgaga aaacggcgtt
     3241 gggtttccta accgatcgcg aatatagcgg aaaatagcaa atgatccgcc caataccttg
     3301 aaagacccga gaccaaaacg ctccgcttcg tccttcaccc aaatgccgcc gatgccaagc
     3361 atattcgcaa gacgcgtcag ccctttgagt ggcgtcatgc gataaccagg catctgtaga
     3421 tggtattttc gagcttcggt cgtgctcgtt gctggcatgt agcgggccac actctcggag
     3481 gagtgcgttt cagcggcgcg tgggttttcc aacca
//
LOCUS       DAOSOR010000604         3507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_26850_length_3507_cov_4.292294, whole genome shotgun sequence.
ACCESSION   DAOSOR010000604 DAOSOR010000000
VERSION     DAOSOR010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26850_length_3507_cov_4.292294"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="PK710_22345"
     CDS             <1..681
                     /locus_tag="PK710_22345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12537.1"
                     /translation="ATANCNDVCETYSELPNAVDHLNCVIGCNDNDCTKGCDQTYPEE
                     GKALDAYFACLGDKCDDECPGFGPSYPICDSGLAMGNESCATCLGDSCCNEVKACEAD
                     QNCLACITGTGGTNCEKDPKMAALETCWGQHCESACKTTGKCTIGFTDPSMAACNDCM
                     KAKCEKECIDASDADGLNAYLKCAQGCTDQACLDNCGTQYPAAAAAIDALDSCLEAKC
                     KTECTTTP"
     gene            935..1678
                     /locus_tag="PK710_22350"
     CDS             935..1678
                     /locus_tag="PK710_22350"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT12538.1"
                     /translation="MGSSRQEVQLALQICQREPLGNECRAEMFENELAAHRVVLSPFW
                     LDRTEVTVQAYQRCVDAGVCSPPPYSRGAQRFDALSLPVSLVSWEDARTFCHFVGKQL
                     PTEAQWERAARGLSARRFPWGQHIATRRANHGTWAADNTDASDGFAELAPVGSFPQGR
                     TTEQVDDLAGNVAEWVSDVYEDGYTNKPVVDPTGPAHGTVRVVRGGSYTHGMPWLRGA
                     ARMYHQASFRAPYLGFRCARRAAPSHPAP"
     gene            1675..2562
                     /locus_tag="PK710_22355"
     CDS             1675..2562
                     /locus_tag="PK710_22355"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT12539.1"
                     /translation="MKIAHLSDPHLLDLDKTACKALLSAKRITGTLNLLAARRRHHHN
                     TVAQQIAHHIRESRVDHVVITGDMTNLSLESEFACVARWLREDLAMPSDCVSIIPGNH
                     DRYTEQAARHHVFESVLQPYIASDIPSASAFPFVRLRGPVAIIGLDSAVPRPVFVAAG
                     EIGHDQRERLRALLHSSEVRQRTPIILVHHSVLWDRPWAREMMHGLRDSAALQALLPP
                     RSGLLLHGHRHRTMCHSIHVGHGRWDVFGVSSASSTSNKPSRAASYHVYEFDDLGNRM
                     GTLSITRRKDGSFRTEELR"
     gene            3059..>3507
                     /locus_tag="PK710_22360"
     CDS             3059..>3507
                     /locus_tag="PK710_22360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12540.1"
                     /translation="MRVSTHSWIAIVGLMFGVVACGSDDDNRGYSPGTGGTGGTNTGG
                     SGGMATGGTGGGQTGGSGGVETGGTGGGQTGGSGGVETGGTGGEETGGTGGATGGTGG
                     ATGGSGGATGGTGGGSSADCELSFPGVPASCNSCFQDKCENQCVDVQS"
BASE COUNT          761 a         1024 c          976 g          746 t
ORIGIN      
        1 gccacggcaa actgcaacga cgtttgcgag acgtactctg aattgccaaa cgctgtggat
       61 cacctcaact gcgtcattgg ctgcaatgac aatgattgca cgaagggttg tgatcagacc
      121 taccccgagg aaggcaaagc gctcgatgcg tacttcgctt gccttggaga caagtgcgac
      181 gatgaatgcc ctggattcgg tccttcttac ccgatttgcg acagcggact cgcgatgggc
      241 aatgagtcgt gcgccacgtg cctgggcgat agctgctgca atgaggtcaa agcatgcgaa
      301 gctgaccaaa actgccttgc ttgcatcacc ggcaccggtg gaacgaactg cgagaaggac
      361 ccgaagatgg ctgccctcga aacctgctgg ggacagcatt gcgaatcggc ctgcaaaacc
      421 accggcaaat gcaccatcgg cttcaccgac ccgagcatgg ccgcctgcaa cgactgcatg
      481 aaggccaagt gcgaaaagga atgcatcgac gcttctgacg ctgacggact caacgcctat
      541 ctaaaatgcg ctcaaggttg tacggaccag gcctgcttgg ataactgcgg tacccaatat
      601 cccgcggcag cggcggccat tgatgccctc gattcctgcc tcgaagccaa gtgcaaaacc
      661 gaatgcacca ccacgccgtg atgcaacttg aatgacgcgg gttcggaaat caccataccc
      721 gcgtctttcc tctcctctcc ttcccgttat cgtctgtgga atcgcgattc acctcgtagc
      781 taggactccc tatcccatcg cacgagcaca gtcagatccg atcgacaccg tacttatttc
      841 tcccggggca agcgcccgca cgctttcggg ccatccgctg accttgaggg tgcctggccc
      901 cgagctgatc ctttttcctc gaagcgtttt caccatgggt tcctcacggc aagaggtcca
      961 attggcccta caaatatgcc aacgagagcc tttgggaaac gagtgtcggg cagagatgtt
     1021 cgaaaacgaa ttggctgctc atcgcgtcgt tctttctccc ttctggctcg accgcacaga
     1081 agtcacggtg caagcctacc aacgttgtgt ggacgctgga gtctgctcac ctcctcccta
     1141 ttctcgtggc gcccaaagat tcgatgcgct ttctctgccc gtatcgttgg tttcatggga
     1201 agacgctcgg actttctgcc acttcgtggg taagcaactc cccaccgaag cgcaatggga
     1261 acgtgcggcc cgtggtctat cagccaggcg ctttccttgg ggccaacata tcgcgacacg
     1321 aagggccaac catggcacct gggcagcgga caacaccgac gcttccgacg gcttcgccga
     1381 actggcgccc gtcggatcct ttccccaggg aagaactacc gaacaagtcg acgacctcgc
     1441 ggggaatgtc gccgaatggg tctccgacgt gtatgaagac ggctacacca acaagcccgt
     1501 cgtcgatccg acgggacccg cccatggcac agtccgagtc gttcgtgggg gaagctacac
     1561 gcatggaatg ccatggcttc gaggagccgc ccggatgtat catcaagcat cttttcgcgc
     1621 accctacctt ggctttcgct gtgcccgtag agccgctcct tctcacccgg ccccatgaaa
     1681 attgcgcatc tctcagaccc tcacctgctc gacctagaca aaactgcctg caaagccctg
     1741 ctttcagcca aacgaatcac gggtaccttg aaccttctcg cggcgcgtcg acgacatcac
     1801 cacaacacgg tcgcccaaca gatcgctcac catatccgtg aaagccgcgt ggatcatgtc
     1861 gtgatcaccg gtgacatgac caatctgtcg ctcgaatcgg aattcgcatg cgtggcccgc
     1921 tggcttcgtg aagatctcgc aatgccgtcc gattgcgtat ccatcattcc tggcaatcac
     1981 gatcgttata ccgagcaagc tgcacgacac catgttttcg aaagcgtgtt gcagccgtat
     2041 atcgccagcg acatcccttc tgctagcgcg tttcctttcg tgcgcttgcg tggaccggtg
     2101 gcgatcattg gcctcgattc agccgtacct cgacccgttt ttgtggcggc gggagagatc
     2161 ggccatgatc aacgcgagcg gttgcgtgca ttgcttcact cttccgaggt tcgtcagcga
     2221 acaccgatca tcctcgttca tcattctgtt ctttgggatc gtccatgggc ccgggaaatg
     2281 atgcatggac tgcgggatag cgcagcgctg caagcgctcc ttccaccgcg ttcgggactg
     2341 ctgctgcacg ggcatcgtca tcgcacgatg tgccattcta tccacgtggg tcatggcaga
     2401 tgggacgtgt ttggcgtgtc gagcgcgtcg agcacatcaa acaagccatc gcgtgcagcg
     2461 agctatcatg tgtacgaatt cgacgacctc ggcaatcgaa tgggaacact atccatcacg
     2521 cgaaggaagg atggctcttt tcgcacagaa gaattgcgat agtgatcctg tgcgatcgcg
     2581 ggcagcgcca tccgcgcttt gccatgatcg aaaacggtgg ctgaccatac acgatcaccg
     2641 catccaccaa gttcctagta cccattcatc gtcgttgccg tgatgacgac agatgagctt
     2701 gatcgaaaat cgatccttcg atgttctcct acgcatcgaa aacggacaca caaatccgat
     2761 tggagaacca cagaggttcc gtttgctccg cgggcaattg gatcgcgttg cgcgcctgag
     2821 gcgatgacat acgacaacgg cactcgcatc atttggccca gcatcaccga ccgctgaaac
     2881 cgaattctgt ttgttactga cgtaagtact tgagaagcat tgcgtgaatc ggttcgtggt
     2941 cgtgttcgaa cttggcggct gaacctattt tcgcccaatt tttttgatca tttggcgtaa
     3001 gaaagagcta tcatcccaac ccctgccgat gcttattggc tttgctggga ggaacgaaat
     3061 gcgagtaagt acacattcct ggattgcgat tgtcggcttg atgttcggcg ttgtcgcttg
     3121 tggaagcgac gacgataacc gtggatactc ccccggaact ggaggcaccg ggggaacgaa
     3181 tacaggaggc tccggaggga tggcaaccgg tggaacgggc ggtggacaaa ccggtggatc
     3241 gggaggagtt gaaaccggtg gaacgggcgg tggacaaacc ggtggttcag gaggagttga
     3301 aaccggtgga acgggcggtg aagaaacggg tggaacggga ggcgctaccg gtggaacggg
     3361 aggcgctacc ggtggttcgg gaggcgctac cggtggaaca ggcggtggct ccagcgccga
     3421 ttgcgaactt agtttccctg gtgtccctgc ctcgtgcaac tcgtgcttcc aagacaagtg
     3481 tgaaaaccaa tgcgtggacg tgcagtc
//
LOCUS       DAOSOR010000605         3503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_26898_length_3503_cov_4.435035, whole genome shotgun sequence.
ACCESSION   DAOSOR010000605 DAOSOR010000000
VERSION     DAOSOR010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26898_length_3503_cov_4.435035"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2339
                     /locus_tag="PK710_22365"
     CDS             <1..2339
                     /locus_tag="PK710_22365"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOT12541.1"
                     /translation="GTGGTGGTGGGTGGTGGTGGGTGGTGGGTGGTGGTGGGSSTCAP
                     LDECSGVCVDFATDPDYCGNCSTSCKPHEACLAGHCQMGVGSLVLSEVRAQAPSFFEI
                     YNGGATSLNLGGYQVQWSTDGFGTGSFELPSYDLGPGEFVTLVEGTGASEPGQLRLGT
                     SVAWISHLAVRLTNAGGYGLDFVRTGSSSVTPPTGTTWSGSNAPNPSAWTNQSLVRNI
                     TKSDTNSAEDWSLVSESSPGSFCPRASQCGGECLDVTNAQHSCGACGFTCSSSQVCLD
                     GSCSQGYTGLWISEYRRSQRAGVEIHNPTPETITLTGYRLDVLGPTTLSYVFPTMTIE
                     PGAFLFLYLGTGVEDNNSLFVGPSAAFGADVAVALYDDAAIPLDFVRFGNSTAAPPSG
                     ASWFGANVPTPDNGFNQSAKRKLDKFDTDGASDWVVNSPATPGYGCVDGLTSCSGSCV
                     DISQNPAHCGGCGNGCGPHETCRGGVCRTVGAVVISELRNASYEYFELFNGTASTVDL
                     SAWEVHWNADGGNGAFVVPSGIKLDPGKFLRLHESSGTSTSEQLMMGTAINWTDWIAV
                     RVLKGTSEIDLVRTGSSPVYGQHWNGVPVDNPPEGQSIVRNIYAADTDSAADWTVQAS
                     DSPSRYCKTANESVCAGQCDDLSKSATNCGACGNACPFGNICNNGVCVEQGAIRMNKY
                     GSFNGTSSGRPEMFFNGRWHVSYNSITTPVATVICRQLGFPNALSGSSSAGSCGSSST
                     CGYLLSITCSGNEALLEDCSFSNYTGDYTGYSVRCEVP"
     gene            2428..>3503
                     /locus_tag="PK710_22370"
     CDS             2428..>3503
                     /locus_tag="PK710_22370"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOT12542.1"
                     /translation="MTARLLVRDIGKSFGATRALDGVTLCAQAGEIHAVLGENGAGKS
                     TLMNILAGSLSADRGFIEVDGAPYRPRSPKEARNLGVAMVHQELALCPHLSVLDNLML
                     GQENARWGWLDRKTMKTRASDALHLVVGPEARSWLDQSVGTLSVASQQLIEIARALVV
                     SAGAGASDCRLLILDEPTSSLGRQDTERLFEILRRLRNQGHTILYISHFLEEIRRIAD
                     RFTILRDGATVGEGVVAEVSVSDMVRAMAGRRVEQRYLRSERRGGPVALTLDRVAGLN
                     LPKQVSLHLHYGEVLGIAGLVGAGRSELLRSIFGLEPIRSGTIKLGAFVGPASPAHRL
                     AHGLGMLSEDRKNEGLAANLSVMD"
BASE COUNT          679 a          943 c         1075 g          806 t
ORIGIN      
        1 gaggcaccgg cggcacgggc ggcacgggcg gaggcaccgg cggcacgggc ggcactggcg
       61 gaggtaccgg cggcacgggc ggaggcaccg gcggcacggg cggcactggc ggaggttcat
      121 cgacctgtgc tccacttgat gaatgctcgg gcgtttgcgt cgattttgcg accgatccgg
      181 actattgcgg caattgttca acgtcttgta agccccacga ggcttgtctc gcaggacatt
      241 gtcagatggg agtcggctcc ctcgtgctat cggaggtgcg tgctcaggcc ccttcgtttt
      301 tcgagattta caacggcggg gcgacgagtt tgaatcttgg cggataccag gtgcagtgga
      361 gcaccgacgg gtttgggacg ggttctttcg aattgccttc ctatgacctt gggccgggcg
      421 agttcgtgac gttggtggag ggaacaggcg cttcggagcc aggacaactt cgtttaggca
      481 cctccgtagc ctggatttcc catctagccg tgcgtctcac gaacgccggc ggatatggtt
      541 tggattttgt acgaacaggc agttcttcgg ttacgccgcc gacggggaca acctggtccg
      601 gttccaatgc accgaatccc agcgcctgga ccaatcagtc gttagttcgt aacatcacga
      661 aatcagatac aaattccgcg gaggactggt ctttggtctc cgaaagctcc cccggtagtt
      721 tctgccctcg ggcctcccaa tgcggaggag aatgccttga tgtgaccaat gcacagcata
      781 gttgtggggc ttgcggcttc acctgttctt catcgcaggt atgcctcgat gggagctgct
      841 cccagggcta tacggggctt tggatcagcg aataccgtcg ttcccaacgc gctggggtcg
      901 aaattcataa tcccacaccc gagaccatca cgctgacggg gtatcgtctc gacgtgcttg
      961 gccccaccac gttgagctat gttttcccca cgatgacgat tgaaccgggc gcgtttctct
     1021 tcctttatct gggcacgggg gtagaggata ataactcact tttcgtgggg ccatcggcgg
     1081 cgtttggcgc ggatgtggcg gtggcgttgt atgatgatgc cgcgattcca ttggatttcg
     1141 tgcgttttgg gaattcgacg gcggctcctc cttcgggcgc gagttggttt ggcgccaatg
     1201 tgcctacgcc ggacaacgga ttcaaccagt ccgccaaacg aaagctcgat aagttcgata
     1261 cggacggcgc ttccgattgg gtggtgaatt ctcccgccac gccaggatac ggttgtgtcg
     1321 atggtctcac atcctgctct ggcagttgcg tggatatcag tcagaatccg gcgcattgcg
     1381 gaggatgcgg caacggctgc ggcccccatg aaacgtgtcg cggaggagtt tgccgaaccg
     1441 taggtgccgt cgtcatctcc gaactccgca atgcatctta cgaatacttc gagctgttca
     1501 atggtaccgc gtcgaccgtg gacttgtcgg cctgggaagt gcattggaat gccgatggtg
     1561 gcaacggagc cttcgtggtt ccttccggca tcaaactcga tcccggcaaa ttcctgaggc
     1621 tccacgaatc cagcggtacg tccaccagcg agcaactcat gatgggaacg gcaatcaatt
     1681 ggacggattg gattgccgtg cgtgtgctca aaggcaccag cgaaatcgac ctcgtaagaa
     1741 caggctcctc ccccgtatac ggtcaacatt ggaacggtgt acccgtggac aatccacccg
     1801 aaggccaaag cattgtccgg aatatctatg cggccgatac ggattccgcc gcggattgga
     1861 cggtgcaagc cagcgatagc ccgtcgcgct attgtaagac ggccaacgag tcggtttgtg
     1921 ccgggcaatg cgatgacctg tccaagagcg ctaccaattg tggcgcttgt ggaaatgcct
     1981 gccccttcgg aaatatttgc aacaacgggg tttgcgtcga acaaggtgcc atccgcatga
     2041 acaagtacgg gagctttaac ggaacctcct ccggtcggcc cgaaatgttc ttcaacggac
     2101 gatggcatgt ctcgtataat tccatcacca ctcccgttgc tacggtgatt tgcaggcaac
     2161 tcggattccc caatgccctg tccggttcgt cgagcgctgg gagctgcgga tcatctagca
     2221 cctgtggtta cctcctgagc ataacctgtt ctggtaacga ggcgttgttg gaggactgtt
     2281 cctttagcaa ttatacgggg gattatacgg gctattccgt tcgctgtgag gttccataac
     2341 cctccttttt ctcttttctc ctcgcgatct tcccactctg gcggatggtt gttgggcttt
     2401 tgtcacggca cggggtagtg tgtcgccatg acggcccgcc tgctcgtgcg cgatattggc
     2461 aagtcattcg gcgccaccag agcgctcgat ggggtaactt tatgtgccca agccggtgaa
     2521 attcacgcgg ttcttggcga aaacggggcc ggaaaaagca cgctcatgaa tattcttgcc
     2581 ggtagtctat cggcagatcg aggtttcatc gaagtcgatg gggcgccgta tcggccccga
     2641 tccccgaaag aagctcgaaa tctaggcgtt gccatggtcc atcaggaact ggcgttatgc
     2701 ccgcatctat cggttctcga caatctcatg ctggggcaag aaaacgcccg gtggggatgg
     2761 ctcgaccgga aaaccatgaa aactcgcgcc agcgacgcgc tccacctcgt ggtcggtccc
     2821 gaagctcgct cctggctcga tcaatccgtc ggtacccttt ccgtggcatc ccagcaactc
     2881 attgaaattg ctagggcttt ggtcgtttcc gccggagccg gggccagcga ttgccgattg
     2941 ctcatcctcg acgaacccac cagcagcctg ggccggcaag acacggaacg cctttttgaa
     3001 attcttcgtc gacttcgaaa tcaagggcat accattctgt atatttcgca ttttcttgaa
     3061 gaaatacgcc gtattgccga tcgattcacc attcttcggg atggggcgac ggtcggcgag
     3121 ggggtagtcg ccgaggtttc ggtgagcgac atggttcggg cgatggctgg tcgacgagtc
     3181 gagcagcgat atttgcgctc cgaacgtcgt ggggggccgg tggcgctgac gctggatagg
     3241 gtggctggat tgaatttacc gaaacaggtg tcgctgcatc tacactatgg ggaggtgttg
     3301 gggattgcgg ggttggtggg ggcggggaga agtgaattgt tgcggagcat ttttggattg
     3361 gagccgattc ggtcgggaac gatcaagttg ggggcgttcg tggggccagc ttctccagcc
     3421 catcgtctag cccacggcct ggggatgctc agcgaagata ggaaaaacga aggcttagcc
     3481 gcgaatttat cggtcatgga taa
//
LOCUS       DAOSOR010000606         3499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_26938_length_3499_cov_3.557491, whole genome shotgun sequence.
ACCESSION   DAOSOR010000606 DAOSOR010000000
VERSION     DAOSOR010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26938_length_3499_cov_3.557491"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="PK710_22375"
     CDS             complement(<1..1268)
                     /locus_tag="PK710_22375"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HOT12543.1"
                     /translation="MLTRAFELGKRFLRGLGTTWVGKIGVALTTSSFLLFSLAEVLRL
                     TGAVTQAYVGLITYMTLPATFVMGLMMIPVGWWLYRRKTGHTTRELLSKEFSDELLAP
                     APLGSRLFLIIAGLTLANIVFLGAGSARMLHFMDQPVFCGTACHSVMHPEWITYQQSP
                     HARVRCVDCHVGQGPEAAVDAKLNGLWQVISVSFNLYERPIPTPVHNLRPARETCERC
                     HWPQKFYGDRIKTIAHFADDRESSPQYTTLSLKVGSGTGQRRGEIHWHVAAENEVRYL
                     PGDKRRNTVQWVEVRRPGGTWKRYTHTQLPVLSQGEQRPHGSKSVRAMDCVDCHNRAT
                     HIYQDPAAAVDDAIERGEIDRTIPFIRRQAVAALRGRYPSDVPATQSIEQDIVGYYAK
                     HEPEVLHEHGRALSKAVEAIERIYSRNIHPT"
     gene            complement(1272..2636)
                     /locus_tag="PK710_22380"
     CDS             complement(1272..2636)
                     /locus_tag="PK710_22380"
                     /inference="COORDINATES: protein motif:HMM:NF024839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOT12544.1"
                     /translation="MKRSWVYVTVVAAAAMVVIPALVLGQDGKKAEPAPAGKCMTCHR
                     SINPGVYHQWYDSEHAKHNVTCIDCHGANKGDPDAFEHEGALVATLVTPKDCGRCHEK
                     EMREVDGSHHAKAGLILESQDAYLAHATGGEPVAVAGCENCHGTNVKVDDKAPNKLSA
                     KSWPNSGIGRLNPDGSKGSCTSCHPRHAFSKVQARRPEACGKCHLGPDHPQKEIYEES
                     KHGNAFFSHVDEMNLKADRWVAGQDYSAAPTCATCHMSATTVRGATHDVGQRLSWTLR
                     PAESALQEDWEKKRNNMKEVCMSCHGPRFVEGHYAQMDGVINLYDQKFAKPAGQIMKM
                     ITDKGLLKNKASFANDIEWVYYELWHHEGRRARHGAAMMGPDYTWWHGLYEVSQHFYF
                     KFIPAARAYNDPEVNAYIDKMFKEDPMHQWMSRPTEEIKADIRSGKMQQFYKDMYTPK
                     RKPE"
     gene            complement(2864..>3499)
                     /locus_tag="PK710_22385"
     CDS             complement(2864..>3499)
                     /locus_tag="PK710_22385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12545.1"
                     /translation="AEPPTQTTQNTDSLPLPLFLQESADMPGLRRELEQLAQRCRAFA
                     DVCDETAKRIDTLAKGQPSHALQRQTQPIAKPLARPLARNLDVKTVPLRVTSPKLKRG
                     EIEVSLDWCDNEPTVIRPQSVATSRTTLPPPVLVACPSCGHGNGQQYESCVMCGCRLH
                     AMTEAEEEEIEVVFTPTLGSRLRNFIQRLDPRRPRWLDPDVLESVDIPSFS"
BASE COUNT          739 a         1085 c          925 g          750 t
ORIGIN      
        1 gtcggatgga tgttgcggga ataaatgcgt tcgatcgctt cgaccgcctt ggacagcgcg
       61 cgaccatgct cgtgcaatac ctctggctca tgcttcgcat agtatccaac gatatcctgc
      121 tcgatcgatt gcgtcgcggg aacgtcggat ggataacgtc cgcgcaatgc cgccaccgct
      181 tgtcgccgaa taaagggaat ggtccgatcg atttctcccc gttcgatcgc atcatccacc
      241 gctgccgcgg ggtcttggta aatatgtgtg gcgcgattgt gacaatcgac acaatccatc
      301 gctcgcacac tcttcgaacc gtgaggacgc tgctctccct gcgacaagac gggaagttgc
      361 gtatgcgtgt agcgcttcca cgtgccaccc ggacgtcgca cctcgaccca ttgcaccgtg
      421 ttgcgacgtt tgtcgcccgg aaggtaacga acttcgtttt ccgctgccac gtgccagtgg
      481 atttcaccgc gccgctgacc ggttccggac ccaaccttga gcgaaagcgt agtgtattgc
      541 ggcgatgact cccgatcgtc cgcgaaatga gcaatcgttt tgatccgatc gccataaaac
      601 ttctgcggcc aatggcagcg ttcacaggtt tcccgcgccg gacgaagatt atgcacgggc
      661 gtgggaatgg gacgttcgta taagttgaac gacacggaaa tcacttgcca cagcccattg
      721 agcttggcat cgaccgcggc ttccggtcct tgtcccacat ggcaatccac acatcgcacg
      781 cgcgcatgag gcgactgctg atacgtaatc cactcgggat gcatcacgct atggcaggcc
      841 gtaccgcaaa acacgggctg atccatgaag tgaagcatgc gcgcgcttcc cgctccaaga
      901 aacacgatgt tggcaagagt cagtcccgcg atgatgagaa acaaacgaga gcccaggggc
      961 gccggtgcca atagctcgtc cgaaaactcc ttcgacaaca actccctggt cgtatgaccc
     1021 gttttacgac gatacagcca ccatcccacc gggatcatca taagccccat cacgaacgtg
     1081 gccggcaacg tcatgtacgt gatgagaccg acgtaggctt gagtgaccgc tcccgtgagc
     1141 cgcagcacct cggccaacga gaacagcaag aaggaactcg tggtcaacgc aacgccgatc
     1201 ttgccgaccc acgtcgttcc caaaccacgc aaaaatcgct tacccagctc gaacgcacgc
     1261 gtgagcatcg tctactccgg cttgcgcttc ggtgtgtaca tatccttgta gaattgctgc
     1321 atcttcccac tgcggatgtc cgctttgatc tcctcggtcg gacgcgacat ccattgatgc
     1381 atcggatcct ctttgaacat cttgtcgatg taggcgttga cttccgggtc gttgtaggcg
     1441 cgggcggcag ggataaattt gaagtaaaaa tgctgactta cctcatacag gccgtgccac
     1501 caggtgtaat ccggacccat catcgccgcg ccatggcgcg cacgacgccc ttcatgatgc
     1561 cacaactcgt aatacaccca ctcgatgtcg ttcgcgaacg aagccttgtt cttcaacaac
     1621 cccttgtccg tgatcatctt catgatctgt cccgccggct tggcgaactt ctgatcataa
     1681 agattaatca cgccatccat ctgcgcataa tgcccctcca cgaaccgcgg gccgtggcat
     1741 gacatgcaga cctctttcat gttgttgcgc ttcttctccc aatcttcctg caacgcagac
     1801 tccgcggggc gaagcgtcca cgaaagcctc tgccccacat catgcgtggc cccccgtacc
     1861 gtcgttgccg acatatgaca cgttgcgcaa gtcggcgccg ccgaataatc ctgccccgct
     1921 acccaccgat ccgctttcaa attcatctcg tccacatgcg agaaaaacgc gttgccgtgc
     1981 ttggactcct cgtaaatctc cttctgagga tgatccggcc ccagatggca cttgccacag
     2041 gcttccggac gacgcgcctg caccttcgaa aacgcatgcc gcggatgaca cgacgtacac
     2101 gagcctttgg aaccgtccgg gttcaaacgt ccaatgcccg aattcggcca gcttttcgcc
     2161 gatagcttgt tcggagcctt gtcgtcgacc ttcacattcg taccgtgaca gttctcacac
     2221 cccgccaccg ccaccggttc ccctcccgtc gcatgcgcaa gatacgcatc ttgcgattcg
     2281 agaatcaaac ccgccttcgc atgatgcgac ccgtcgacct cccgcatctc cttttcatga
     2341 caccgaccgc agtctttcgg cgtcacgagc gtcgccacca acgccccctc atgctcgaaa
     2401 gcatcaggat cacccttgtt cgccccgtgg cagtcaatgc acgtcacgtt atgcttcgca
     2461 tgctcggaat cgtaccattg atgatacacc cccggattga tcgaacggtg gcaggtcatg
     2521 cacttgcccg ccggcgccgg ttccgctttc ttaccgtctt gacccaatac caaggccggt
     2581 atcaccacca tcgctgcagc ggcgacgacc gtgacgtaaa cccaacttcg cttcatcgtg
     2641 ctcctccagt tggaccgttg ggtctaacct aggcggaggc ttcggtttag tctacgaaat
     2701 acgaaggtta ttccgcgttt gccagccaac ggagcgttgc cgcaatcggc ctgcgttcac
     2761 gatttggaca gggcggtgaa gacgcttcga tatcgaggga ttgggaggcg atgcggacac
     2821 cgaagagaag tgttccgatt cgtctgtaaa tatgcgtaat tattcacgaa aatgagggta
     2881 tgtccaccga ctccagcaca tccggatcga gccaacgcgg acgtcgtgga tccagacgtt
     2941 ggatgaagtt gcggaggcgt gaacccagag tcggcgtaaa aacaacctcg atttcctctt
     3001 cttcggcctc ggtcatcgca tgcagccgac acccgcacat gacacaactt tcgtattgct
     3061 gcccgttgcc atgaccacac gacgggcatg ccacgagcac aggaggcggc aaggtcgtcc
     3121 gagaagtggc cacggactgc ggacgaatga ccgtgggctc attgtcgcac caatcgagcg
     3181 aaacctcgat ctccccgcgt ttgagcttgg ggctggtcac gcgaaggggg acagttttta
     3241 cgtcgagatt tcgagccaaa ggacgggcaa ggggctttgc gatgggctgc gtttgacgct
     3301 gaagcgcgtg cgatggttgt cccttcgcca acgtatcgat gcgttttgcc gtttcatcac
     3361 aaacatccgc gaaggcgcgg catcgttggg cgagttgctc gagttcacga cgaagccccg
     3421 gcatgtccgc cgactcctga aggaagaggg gaaggggaag cgagtcggta ttttgtgtgg
     3481 tttgtgtcgg tggctcggc
//
LOCUS       DAOSOR010000607         3492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_27000_length_3492_cov_4.367472, whole genome shotgun sequence.
ACCESSION   DAOSOR010000607 DAOSOR010000000
VERSION     DAOSOR010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27000_length_3492_cov_4.367472"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="PK710_22390"
     CDS             complement(<1..1304)
                     /locus_tag="PK710_22390"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOT12546.1"
                     /translation="MPTTRFPRYFNIFHDKGAFAGLFFAALWAAACSSPPSYPTSPTE
                     TPPSIRPNAPGDLAPPLFTEEKRNSVFAGVAQITGTGFAPGMGPTCTGFLLDTSAPNA
                     PAYVITNGHCVDILDAITVLSGFPADGLASFARFANPEGTVAPNTIDVRVNEIAWASM
                     RGTDTAVLRLDRTLGSLTEAGIPSYRIRGFAKPGEAVHVVGIPIDGVPSEQQVLRRVD
                     CTAHAPSRLLEHVWLWDQSQGGDCWGIRQGSSGSPVFDRNQQVVGVLNTTTIGAEKGG
                     ECYLGNPCEVGPAGTLHRDNTSYWMSLGELPGCFDDKGVFSLEQAGCPLEAGPPYQVL
                     NANAYARPPAHFSLTVNSTATDPIVFKTGPLETTDCRSPEGYGGSLEMGALYEAEINA
                     SKGHVLFCATGKNSDGQLALRQAGFAVVEIDTTAPVRPIQLTV"
     gene            1522..1896
                     /locus_tag="PK710_22395"
     CDS             1522..1896
                     /locus_tag="PK710_22395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HOT12547.1"
                     /translation="MRFIVYHYPKCATSRKALAWLDKHEIAYEAIDLVATPPSKEKLK
                     DLWKRSGEPIRKLFNTSGESYRKGGFKDKLATMSDADALTALAADGKLIKRPLVDMGQ
                     RVIVGFREEDYAQAFAGLDSAK"
     gene            complement(2239..2907)
                     /locus_tag="PK710_22400"
     CDS             complement(2239..2907)
                     /locus_tag="PK710_22400"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOT12548.1"
                     /translation="MTPLVAGTLAGAFHVISGPDHLVAVTPLAVRQPAQAVRVGALWG
                     AGHAVGAGLLGAIGVLAKSYLDVDFLSSWAEFLVGAVLCAVGIWAIVQTRKLLIHHHH
                     HAHDGEVDAVAEHEHEHLHIGDDKKHLHHGTSFGVGVLHGAAGTGHLLGVLPSLALPP
                     LSAAVYLISYGLAAVGAMAAFSAGLGLLGKRLRPAWLRHFLMGSGVVAIGLGVYWMWT
                     GWPG"
     gene            complement(2904..>3492)
                     /locus_tag="PK710_22405"
     CDS             complement(2904..>3492)
                     /locus_tag="PK710_22405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12549.1"
                     /translation="TSFEVPAKAWTSTGFVVGPADDTTQLGEGATQQDLEAMKAGGYS
                     IHVKGSASKNGVTKTFDWQFKTKTLYRDCKAEVDGKEVHGVVVPQGGEDTVELTIHGD
                     HFFYDDLEAATAERRFDAIACADANGDGEVTLEELEQATLLPDANATDEACKNRPVYR
                     PGSQPVNNLRAFVTALSRTVGHYRGEGECISEKME"
BASE COUNT          754 a         1005 c         1053 g          680 t
ORIGIN      
        1 acggtgagtt ggatggggcg cacgggggcg gtggtatcga tttcgacgac ggcgaatccg
       61 gcttggcgaa gggcaagctg cccatcgcta ttttttcccg tggcgcagaa caggacgtga
      121 cctttcgatg cattgatttc cgcttcgtac agggcaccca tctcgagcga tcccccatag
      181 ccctcgggcg atcgacagtc ggtagtttcg agggggcccg tcttgaaaac gatcgggtcc
      241 gtggccgtgc tgttcaccgt gagggaaaag tgggcgggcg gacgagcgta ggcgttggca
      301 ttgaggacct ggtacggtgg accggcttcg agggggcagc cggcttgttc gagggaaaac
      361 acacctttgt cgtcaaagca tcccggcaac tcgcccagcg acatccaata actcgtgtta
      421 tctcggtgta acgtgcctgc cgggccgact tcacaggggt tgccaagata gcattcgcct
      481 cctttttccg cgccgatggt ggtggtattg agcacgccaa cgacctgttg gttacggtcg
      541 aagacgggcg agccggaact gccctggcga ataccccagc agtccccccc ttgggattgg
      601 tcccacaacc atacgtgttc gagaaggcga gagggagcgt gggccgtgca gtcgacgcgt
      661 cggagaacct gttgttccga cggcacccca tcgatgggga ttcccacgac gtggacggct
      721 tccccgggtt tggcgaagcc gcgaatgcga tagcttggga tgccagcctc ggtcaacgac
      781 cccagggtgc ggtcgagacg cagcacggcg gtatccgttc cgcgcatcga agcccaggcg
      841 atttcattga cccgaacgtc gatggtattc ggggccacgg ttccctcggg gttggcgaag
      901 cgtgcaaaag aagcgaggcc gtcggcggga aagccgctaa ggacggtaat ggcatcgagg
      961 atatcgacac aatgaccgtt ggtgatgacg tacgctgggg cgttgggggc gctggtatcc
     1021 agcaaaaagc cggtgcaagt ggggcccatg cccggtgcga atccggttcc ggtgatttgc
     1081 gccaccccgg cgaatacact atttcgtttt tcttcggtga aaaggggtgg tgccaggtcg
     1141 ccgggggcgt ttggacgaat cgagggcggt gtttctgtgg ggctggtggg atatgatggg
     1201 ggtgaggaac aggcagcggc ccacaaagcc gcgaagaaaa ggccagcgaa agcccctttg
     1261 tcgtggaaga tattgaaata gcgaggaaat ctagtggttg gcatggttgt tttgcgatgg
     1321 tggagggggt aaaaggaatg acaagtcctt gcagtatggg gtggcgatgg ccgacggcaa
     1381 gagggggaga agggtgaaat tacaatggtg aaagcgcggt tggcaccttc gatgaagcga
     1441 ctggcagcgc tggctgccaa tagcccaagg aactgtcgtt ttcatcatat tgggcaagca
     1501 agcccgtgta ggatgccctc catgaggttc atcgtttacc actatcccaa atgcgcaacg
     1561 agccgcaaag cgttggcgtg gctcgacaag cacgagatcg cctacgaggc catcgatctg
     1621 gtggcgactc cgccatcgaa agaaaaactg aaagaccttt ggaaacgttc cggggagccg
     1681 attcgcaagc tattcaatac ctcgggggaa tcctaccgga aggggggatt caaggataaa
     1741 cttgccacga tgtccgatgc cgatgctctg acggctctgg cagccgatgg caaactcatc
     1801 aaacggcccc tcgtcgacat gggacagcgg gttatcgtgg gtttccgaga ggaagattac
     1861 gcccaagcgt tcgcaggtct tgattcggcc aagtgagcag accttgggcc cgcgagcctg
     1921 gaaacatcac cgtggccgac catggacgac ccgatcatcg cggcgcgtga aaacggtagg
     1981 aaatcatgaa acgttgtgtg ttgaatgatt gtgggaggat gtgttgagga ccatccaagg
     2041 attggatgcg caacgtattg tgcattaaca taaaacattg cgccactatc gactttttct
     2101 acgcctctgg tgctgggggc cgtttaccca agctcatcaa gttcgcgtcg tgctctcgct
     2161 cgccatgaag ggggggtgaa agggggtgat cgaggaagag ggtccgaaaa gcagacccga
     2221 caagcacatg gtggagggtt aacccggcca gccggtccac atccaataca cgccaagccc
     2281 aatggccacg actcccgagc ccatcaaaaa atgccgtagc catgcggggc gaagccgttt
     2341 tcccaacaac cccaaccccg cgctgaacgc agccatcgcc cctaccgccg ccaacccata
     2401 agaaatcagg tacacggcag ccgacaaggg cggaagcgcc aacgatggca gcacgcctag
     2461 caaatgcccg gtgcccgcag ccccatgcaa gactccaaca ccaaacgatg tgccgtgatg
     2521 aagatgcttt ttgtcgtcac cgatatgcag atgctcgtgc tcatgctccg ccacggcatc
     2581 gacctcgccg tcgtgagcgt gatgatggtg atggatcagc agctttctcg tctgcacgat
     2641 ggcccaaata cccaccgcgc ataagacggc tccgaccaaa aactcggccc acgacgacaa
     2701 aaaatcgaca tcgaggtagg atttggcgag gactccgatg gctccgagca acccggcgcc
     2761 taccgcatga cccgcgcccc ataaagcacc gactcgaacg gcctgggccg gttgacgcac
     2821 ggccagcggc gtgaccgcta ccagatggtc cggtccggaa atcacgtgaa aggcacccgc
     2881 tagggttccc gccaccaacg gggtcattcc attttctccg agatgcactc tccttcaccc
     2941 cggtaatgac ccaccgtgcg agacagggcc gtcacgaacg cgcgaagatt gttgaccggc
     3001 tggctgcctg gacggtacac cggcctgttc ttgcacgctt cgtccgtggc gttggcatcg
     3061 ggaagcaacg tcgcttgctc cagctcttcc aacgttactt cgccatcacc gttcgcgtcc
     3121 gcacaggcaa tggcatcaaa ccgtcgctcc gcggtcgcgg cttccaagtc gtcgtaaaag
     3181 aagtggtctc cgtggatcgt caactccacc gtatcctctc ccccttgcgg caccaccaca
     3241 ccatgcacct ctttgccatc cacttccgcc ttgcaatccc gatacagcgt cttcgtcttg
     3301 aattgccaat caaacgtctt ggtcaccccg tttttcgaag cgctcccttt cacgtgaatg
     3361 ctgtaccccc cagctttcat cgcttcgaga tcctgctgcg tcgccccttc gcccaattgt
     3421 gtcgtatcat cggccgggcc taccacgaaa cccgtggaag tccacgcttt ggcaggtact
     3481 tcaaaagacg tg
//
LOCUS       DAOSOR010000608         3491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_27010_length_3491_cov_3.855064, whole genome shotgun sequence.
ACCESSION   DAOSOR010000608 DAOSOR010000000
VERSION     DAOSOR010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27010_length_3491_cov_3.855064"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="PK710_22410"
     CDS             complement(<1..1162)
                     /locus_tag="PK710_22410"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT12550.1"
                     /translation="MSPMYEASPPRPILSLGRYALYGEIAAGGMATVHFGRMSSLGGF
                     SRTVAIKRMRQEIARDPVFVAMFLDEARLTSRIEHPNVVATLDVIAEQGEVFLVMEYI
                     RGESLQRLRRAAKSRQEMVPVPVAVSIMGQVLAGLHAAHEALGEGGTPLGIVHRDVSP
                     QNIMVGEDGISRIMDFGIAKAESRLQITRDGQMKGKLPYMAPEQLSGGRSKIDRRVDI
                     FSSAVVLWELLASRSLFRADHPGETLANVLSAPIPSLTAIRSDIPSTLDHVMQRALSR
                     SPEQRPQTAEQFASELEESIQAGPASPRVVAAWVASLAGEVLVNRTQYVREMESLSSH
                     AMADGVAEGMARGPEPSFTHESNSEAVAAFGPLPPPVAPPFKRSASSSIDLAS"
     gene            complement(1250..2605)
                     /locus_tag="PK710_22415"
     CDS             complement(1250..2605)
                     /locus_tag="PK710_22415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOT12551.1"
                     /translation="MAGRKTPRGPEASLFEAAAKQSDQQSRPLADRLRPRDLEEVVGQ
                     SHLLGSGKPLERAIATDKIPSMLLWGPPGTGKTTIARVIATRTQAHFVPYSAVLGTVG
                     ELRERLQEAKEAKLFHGRRTILFIDEIHRFNKSQQDAFLPFVEEGIVTLIGATTENPS
                     FSVNAALLSRCKVFRLEGLDEASLILLLERAMHDEHRGWGRSAIDYDPLALAAIARLS
                     DGDGRRALNTLEMILQQLQPDESLTVQVVTRSSEHRPLLYDKSGEEHYNVVSALIKSM
                     RGSDPDASIYWAMRMLEAGDDPLFILRRLLIFASEDIGNADPRALQVAVAADQSFRRM
                     GMPEGIYPIAHAVLYLACAPKSNAVGTAFSQAKDAIDEFGSLAVPHKLRNAVTVLMKS
                     VGYGQGYRNPHEEAGHVASGERYLPDELEGRRFYEPSNQGLEIAIGEKLARLRNPKPQ
                     G"
     gene            complement(2613..>3491)
                     /locus_tag="PK710_22420"
     CDS             complement(2613..>3491)
                     /locus_tag="PK710_22420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOT12552.1"
                     /translation="AEESQRVRGDLSDPLGEREHEVAPRLVHRYPDRALLLVTSTCAV
                     HCRYCTRARLVGTEDGYWSLSELEPAFRWLEAHPQVREVLISGGDPLVVSTRRLDALL
                     WRIRRISSIDVIRVGTRVPNVLPMRVDDELVTMLRAHQPLWIMTHFNHPRELTALSCH
                     ALAQLVDGGFPVMNQTVLLRGINDDDQVLATLFRGLVRQRVRPYYLLHADVMEGTSHF
                     RTSVARSLEIYSRLQGKVSGLAIPRLMVDTPGGKGKVPLGPASVVSMESHKTVLRTFR
                     GEEVVLIEPGQFEGHR"
BASE COUNT          769 a         1065 c          946 g          711 t
ORIGIN      
        1 gggatgcgag gtcgatgctc gaagaagcgg aacgtttgaa ggggggagcc actggcggag
       61 gtagcggacc gaaggcggcg acagcctcag aattggattc gtgggtgaaa gagggctccg
      121 gtcctcgggc cataccctcc gcaaccccat cagccatggc gtgcgatgac agggattcca
      181 tctcgcgaac gtattgggtt cggttgacga gcacctcgcc ggcgagagag gcaacccacg
      241 cggcaacgac tcggggcgag gctgggcctg cttggatgga ttcctcgagt tcagaagcga
      301 attgctcggc ggtttgcggc cgctgctccg gacttcgact tagcgcccgt tgcatcacat
      361 ggtcgagcgt cgatgggata tccgagcgga tggcggtcaa cgaaggaatc ggcgcggaca
      421 agacattggc caacgtttcg ccgggatgat cggcgcgaaa caggctccgc gacgcaagaa
      481 gctcccaaag cacaaccgcg gaagagaaaa tatccactcg tcgatcgatt ttcgaacgcc
      541 ctcccgacaa ttgctcgggc gccatatacg gcaacttccc cttcatctgg ccatcgcggg
      601 ttatctgtaa tcggctttcc gcctttgcga ttccaaagtc catgatgcga gaaataccgt
      661 cttctccaac catgatgttt tgcggggata cgtcacgatg aacgataccc aagggcgttc
      721 ccccttcccc caacgcctca tgggccgcgt gaagaccggc gagaacctgg cccatgatgc
      781 tcacggccac cggtacggga accatctcct gacgtgactt tgcggctctt cgcagacgct
      841 gcaaggactc accgcggatg tattccatga cgagaaagac ctcgccctgc tcagcgatga
      901 catcgagggt agcaacgacg ttcggatgtt cgatgcgtga ggttagccgc gcctcatcga
      961 ggaacatggc cacgaagacg ggatcccggg caatctcttg gcgcatgcgt ttgatggcga
     1021 cggtccggct gaagcctccg aggctgctca ttcgcccgaa atgtacggtt gccatgcctc
     1081 cagccgcaat ctccccgtac agcgcatagc gccctaggga gaggatcggc cgaggcggcg
     1141 atgcctcgta catcgggctc attattgata cggtacaatg gagaaatcaa aacgtccagc
     1201 ttgggtgcaa acccattccc ccgcaaataa cgctgagatg ctgatgcttt cagccttggg
     1261 gttttggatt tctcaaccgc gccaactttt ctccaatggc gatttccaac ccttgattcg
     1321 agggctcata aaacctccga ccctccaact catcgggaag ataacgttcc cccgacgcta
     1381 cgtgcccggc ttcttcatgc gggttgcggt atccctggcc gtagccgacc gacttcatca
     1441 ataccgtgac ggcattgcga agcttgtggg gcaccgcaag gctgccgaat tcgtcaatgg
     1501 catcttttgc ctgggaaaaa gccgtcccca cggcattgga tttgggggcg caagcaagat
     1561 agagcaccgc atgcgcgatc ggatatatcc cctcgggcat tcccattcgg cgaaaggatt
     1621 gatccgccgc caccgccact tgcaaggccc ggggatcggc gtttcctatg tcttcactcg
     1681 caaaaatcaa cagcctgcgc aaaataaata atggatcatc gcccgcttcg agcatgcgca
     1741 tcgcccaata aatcgacgca tccggatccg acccccgcat ggacttgatg agagcactga
     1801 ccacgttgta atgctcttcc ccgcttttat catacaataa cggtcgatgc tcactggacc
     1861 gagtcaccac ctgcaccgtc aatgactcgt cgggttgcaa ttgctgcaaa atcatctcga
     1921 gcgtattgag ggcgcgacga ccatcgccat cggatagcct ggcaatggcg gccaacgcca
     1981 gcggatcgta atcgatggcg gaccggcccc aaccgcgatg ttcatcgtgc atggctcgtt
     2041 cgagcagcag tatcagcgac gcctcatcga gtccttcgag ccgaaacacc ttacagcgcg
     2101 agagtaaggc cgcattgacg gagaacgaag ggttctcggt agtagctccg atcagcgtta
     2161 cgattccctc ctcgacgaaa ggcaaaaagg catcttgttg gcttttgttg aatcggtgga
     2221 tttcatcgat gaacaaaatc gtgcgacgcc cgtgaaagag cttggcttct ttcgcttcct
     2281 gcaatcgttc tcgcaattcc cccacggttc ccaacacggc ggaataggga acgaaatggg
     2341 cttgggttct cgtggcgatc acccttgcga tcgtggtttt tcccgttcct ggggggcccc
     2401 acaacagcat cgaaggaatt ttatcggtgg cgatagcgcg ctcgagaggc tttcccgaac
     2461 cgagcaagtg cgattgtccg acgacttcct cgagatcgcg ggggcgaaga cggtccgcca
     2521 acggtcgaga ctgctggtcc gactgctttg cagccgcttc gaaaagcgat gcctcgggac
     2581 ctcgcggagt tttccgacct gccatgcttc cctcaccgat ggccctcgaa ttgtccgggt
     2641 tcgatgagga ccacctcttc gccacgaaag gttcgcagca ccgttttgtg cgattccatc
     2701 gataccacgg acgctgggcc tagcggtacc ttgcctttgc cgccgggcgt atccaccatc
     2761 aatcgcggta tcgccaaccc agacaccttt ccttgtaatc tgctgtaaat ttcgagactt
     2821 ctcgctaccg aggtccgaaa atgcgacgtt ccttccatca catccgcgtg caacaaataa
     2881 taggggcgca cccgttgtcg caccaacccg cgaaaaagcg tcgcaagcac ctggtcgtca
     2941 tcattgatgc cacgaagaag caccgtctga ttcatgaccg gaaaccctcc atccacgagc
     3001 tgcgccaaag catgacacga caatgccgta agctctcgtg gatggttgaa gtgcgtcatg
     3061 atccatagcg gctgatgagc ccggagcatc gtcaccaact cgtcatccac acgcatgggc
     3121 aggacattcg gcacgcgcgt gcccacccga atgacatcga tgctcgaaat acgccgtatt
     3181 ctccaaagca acgcatccag tcggcgcgtg gaaaccacca ggggatcccc tccgctgatc
     3241 aacacctcac gtacttgcgg atgagcttcc aaccatcgaa aagcgggctc caattctgac
     3301 aaagaccaat atccatcctc cgttccgacc aatcgtgccc gtgtgcaata tcggcaatgc
     3361 accgcgcatg tggacgttac caacaacaac gcgcgatccg ggtagcgatg caccaacctg
     3421 ggcgccacct cgtgttctcg ctcacccaat gggtcggata agtctccgcg aacccgttgc
     3481 gactcttctg c
//
LOCUS       DAOSOR010000609         3489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_27033_length_3489_cov_3.625510, whole genome shotgun sequence.
ACCESSION   DAOSOR010000609 DAOSOR010000000
VERSION     DAOSOR010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27033_length_3489_cov_3.625510"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /gene="dmeF"
                     /locus_tag="PK710_22425"
     CDS             complement(<1..818)
                     /gene="dmeF"
                     /locus_tag="PK710_22425"
                     /inference="COORDINATES: protein motif:HMM:NF033827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HOT12553.1"
                     /translation="MVSGHDHSFGQERKRPGERKTIIVIVLTAVTMVVEIVAGLVYGS
                     MALLADGLHMASHAAALTISAAAYVYARRHASDRRYSFGTGKVNALAGFTGSVLLGLF
                     AGFMAFESVGRIISPVDIVFDHALFVAVVGLAVNAISVVFLQGGHGHSKDEPNHDASD
                     HDVHEDHNLRSAYFHVLADALTSVAAIVALLAGKYFGWVWLDPIMGIVGALLVGKWSL
                     GLVRDTSAVLLDRQAPEPMVARVRSAIEPEARVVDLHIWSIGPGIYACVLAVEGP"
     gene            complement(994..1866)
                     /locus_tag="PK710_22430"
     CDS             complement(994..1866)
                     /locus_tag="PK710_22430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOT12554.1"
                     /translation="MSALLDAAFLKELEALRRRLEIKARSGSAGDQLAKRRGGSAEFQ
                     DHRPYAPGDDLRRIDWAAYARTGEPVLKLFRMEEDVMLRLLVDSSASLDYGVPSKFDI
                     ARRVGAAIGYMVMASSQRAQVFSVGEGIKSMSRSARGRGGVASFLKSLEALQPGGATH
                     LSKAIDAVVQRSHRPGMLVVISDFLDGGLVTASLSRAKSAGHDLALVHVVTPEEVEPP
                     WEGDWTLEDGETGATVDVTMDPAALEAYMLRFTGLCEELRAVARKLGAGYVRMRTDEP
                     LEPVVRRFVARGVD"
     gene            complement(1863..>3489)
                     /locus_tag="PK710_22435"
     CDS             complement(1863..>3489)
                     /locus_tag="PK710_22435"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT12555.1"
                     /translation="ELRQAARLGRTPIILMTITALVALLMTAIGGIMSVAASPSTIGI
                     TLFHTFFSLAFFVVTLLGPALAANSVASEREGRTWEAVILTGLPPKTIARGKFLSAFT
                     NISMYIVMLAPVGALSFLFGGVTALEVVVAFFFLFLFALLGVAFGLAISSALASGRTA
                     ILLSLLLAFPLTLVAYLGLGVGLSVAANDAWPAVIGGAPVWLPTAYERAPFSLEYITF
                     LVVIPIAGLTVPAWFFYEITVANLTSLTDDRATGLKRWYVVMVPLLTAAIVIPVVTLG
                     NGKPSGSIMVGVSILFSFVVFAFFVFQGDAIGPSRRVLVHWERKKASQLTRFMGPGLV
                     KTISLVFVLGSVGILVLAVAGVAVSLLSPHVTDRQLEAERVVAFVEYAATYLLFLGGF
                     AAWIRTKASTSGVARVLLLVAIFLSAAGPWIVAAIAGVISEGSDEALIVAAPSPFFAF
                     VLVGMLDKAQRGLATIVGVICSSAWALIGLGLFAAASVRAKRIIHEHFAMLSESEKML
                     AQEDEELQRMDLQQQNLDSAPENALVGQGESGA"
BASE COUNT          905 a         1186 c          811 g          587 t
ORIGIN      
        1 ggcccctcca ccgcaagcac acacgcgtaa atccccggac caatggacca tatatgcagg
       61 tcgacgaccc gcgcttctgg ctcgatcgcg cttcgcactc tcgccaccat cggttccggc
      121 gcttgccgat ccaacaacac ggcgctcgta tcgcgcacaa gacccaaaga ccattttccc
      181 accaacagcg cgcctacaat ccccatgatc ggatccaacc acacccaccc gaagtacttg
      241 ccagcaagca aagccacaat cgccgccact gacgtcaacg catcggccaa tacgtgaaaa
      301 tacgctgacc gaagattatg gtcctcatgc acatcatgat ccgaagcgtc atgattcggt
      361 tcatccttcg aatgcccatg ccctccttgg agaaatacca ccgatatcgc attgaccgcc
      421 aaccccacca cagccacaaa aagcgcgtga tcaaaaacga tatcgacggg cgaaatgatc
      481 cgccccacgc tttcgaacgc catgaagccg gcaaacaatc cgagcaatac cgatccagta
      541 aaacctgcca gcgcgttgac cttgccggtc ccaaaactat aacgacgatc cgaggcgtga
      601 cgtcgggcat acacgtaggc agccgccgaa atcgtcagcg ccgcggcatg cgatgccatg
      661 tgcaacccat cggcaagcaa cgccatcgag ccatagacca atcccgccac gatctcgacg
      721 accatcgtga ccgcggtcaa cacgatgacg atgatggttt ttcgctcacc aggccgcttc
      781 cgctcttgcc caaaggaatg atcgtgaccc gaaaccatcg tcgcttgaaa tccgtttatg
      841 ctcgcgatga ccgtcttgct cgcaagccca tgagatccaa cacagccact tcgcttgtaa
      901 tacagcttcg atcaagatgg tacggccccc gtcgtcggag cagcgcctca acgccccatc
      961 agcacaccac ccacaggttg cgatcgtatg gcctcagtct accccccgag ccacgaaacg
     1021 acgtacgacc ggctccaaag gctcgtccgt tcgcattcgc acatatccgg ctcccagctt
     1081 gcgcgccacc gctcgcaatt cttcacataa ccctgtaaat ctcaacatat acgcctcgag
     1141 ggccgccgga tccatcgtca cgtccaccgt cgcgcccgtc tccccatcct cgagtgtcca
     1201 atccccctcc caaggcggct ccacctcttc cggcgtcacc acatgcacca acgccaagtc
     1261 gtgacccgcg gactttgccc gactcagcga cgccgtcaca agccccccat ccaaaaagtc
     1321 gctgatgacc acaagcatcc ctggacgatg ggaacgttgc accaccgcat caatcgcttt
     1381 ggaaagatgc gtcgctccac ctggttgcaa cgcttccaat gacttgagaa aggatgccac
     1441 accccctcgc ccccgggcgc tacgactcat gctcttgatc ccctcaccga ccgaaaacac
     1501 ctgagcacgc tgcgagcttg ccatcaccat gtacccaatc gcagccccca cccgtctggc
     1561 gatatcgaac ttcgaaggaa ctccgtaatc caacgatgcc gatgaatcca ccagcaaacg
     1621 aagcatcacg tcttcttcca tccgaaacag cttgagcaca ggctcgcccg tcctcgcata
     1681 cgccgcccaa tcgatgcgtc gtaaatcatc gcctggcgca tacgggcgat ggtcctggaa
     1741 ctccgcggag cctcctcgac gctttgcgag ttgatctccc gccgaccccg accgcgcctt
     1801 gatttccaga cgccgtcgta acgcctctag ctctttgaga aaagcggcat cgagcaacgc
     1861 gctcatgccc ccgattcccc ttgcccaacg agggcgtttt cgggtgcgga atccaaattt
     1921 tgttgttgta aatccatacg ttgcaattct tcgtcttctt gcgccagcat tttttcggat
     1981 tccgaaagca tggcgaagtg ctcatggatg atccgcttcg ctcgcaccga tgccgccgcg
     2041 aacagtccca atccaatgag cgcccaggcc gaagaacaga tgaccccaac gatggtcgcc
     2101 agcccacgtt gggccttgtc gagcatgccg accaaaacaa atgcaaaaaa cggtgaggga
     2161 gccgcaacga tcagcgcttc atcacttcct tccgaaatga cccccgcaat cgccgcgaca
     2221 atccaaggtc ccgcggcaga caaaaagatg gcaaccaata gcaacacgcg cgctacgccc
     2281 gaagtagaag ctttcgttcg aatccatgcg gcaaacccac caagaaacaa taagtaggtg
     2341 gccgcatatt caacgaaggc taccacacgc tccgcttcaa gttgccgatc ggttacatgc
     2401 ggcgacaaca gactgaccgc cacacccgcc accgccaaca cgagtatccc aacactaccc
     2461 aacacaaata ccaaggaaat ggtttttacc aatccgggcc ccatgaaacg cgtaagctga
     2521 cttgcttttt ttcgctccca atgcacgagc acccgccgcg atgggccgat ggcatcccct
     2581 tgaaaaacga agaaggcaaa cactacgaac gaaaacagaa tcgacacacc caccatgatc
     2641 gaaccggatg gcttgccatt ccccaaagtt acgacgggaa tgacgatggc ggccgtcaac
     2701 agaggaacca tgaccacgta ccatcgcttc aaccccgtgg cgcgatcgtc ggtcaagctg
     2761 gtgagattgg cgacggtgat ctcgtaaaaa aaccaggcgg ggaccgtcaa tcctgcgatg
     2821 gggatgacga ccaagaaggt gatgtactcg agagaaaagg gagcgcgttc ataggccgta
     2881 gggagccata cgggagcgcc gccaatcacc gctggccaag catcgttggc cgccacggaa
     2941 aggccaacgc ccaaccccaa gtaggccacg agcgttagcg gaaacgcgag cagaagggaa
     3001 agcaaaatcg cggtacgacc actagccaat gccgaactga tggcaagacc gaaggcgacc
     3061 cccaacaagg caaaaagaaa caggaaaaaa aacgcgacca ccacctctaa cgcggtcacc
     3121 ccaccgaaaa gaaacgacag cgcccccacg ggcgccaaca tcacgatgta catcgatatg
     3181 ttggtgaaag ccgacaaaaa ctttccccgc gctatcgtct tcggaggcaa gcctgtcaga
     3241 atcaccgcct cccacgttct gccttctcgc tccgatgcca cactgttggc cgccaaagcc
     3301 ggtcccaaca aggtcaccac gaaaaacgca agcgagaaaa acgtgtgaaa taacgtaatt
     3361 ccaatggtgg aaggcgaagc ggccacactc atgatgccgc caatcgcggt catgagcaaa
     3421 gccaccagcg ccgtaatcgt catcaggatg ataggggttc gccctaagcg cgccgcttgg
     3481 cgaagttcc
//
LOCUS       DAOSOR010000610         3482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_27110_length_3482_cov_4.148526, whole genome shotgun sequence.
ACCESSION   DAOSOR010000610 DAOSOR010000000
VERSION     DAOSOR010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27110_length_3482_cov_4.148526"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1424)
                     /locus_tag="PK710_22440"
     CDS             complement(135..1424)
                     /locus_tag="PK710_22440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12556.1"
                     /translation="MSLLVILGVGAGLLSIGRPGDAQSPSPTTSSVIPTSAVSAADAD
                     VDADTDVDALVPAPGTEMARVDRLRCGPEPDDWVKRIRAVPGARVDTRNNIDIDGDGT
                     PDRIISVRRGCQTSKAVHHILLKRPSCWAYLGHILATSLSVEGPARPLRTIVARGAEA
                     GRGGQATGGTVRFDFEPYGLQFEPRRGPDPANDCISRDDDPENWRPLPDLDGDGIPDA
                     VHVTVVRNLIPIIIETIRVRRGACWRSVATVESGYDGKPGLASHVLLTSPRHRAKDIL
                     LADGTVFVWNGSSYDERVMALAFDADSIAGPPPTTAQTCTTTAPTGSLLPVGDLDCDG
                     VTDFVSRTGEELCCPPQTYWSYYVSNHGCWQSQSVRLYGSYGRLPLPANAGFRTEKMD
                     ENGVRELEIRTWTVRYQECFCAPVAGKCPKQCDLSSN"
     gene            complement(1492..1797)
                     /locus_tag="PK710_22445"
     CDS             complement(1492..1797)
                     /locus_tag="PK710_22445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12557.1"
                     /translation="MRKNHFALFAALLSTVGLCELVRFTPIVVPSLVKLQAFPSADPE
                     VRRVVEPNTPLAWNVLNGIAKIPICRKFVDAPASGETDRGERRTNGSAGNKTLVCGC"
     gene            complement(2232..2852)
                     /locus_tag="PK710_22450"
     CDS             complement(2232..2852)
                     /locus_tag="PK710_22450"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT12558.1"
                     /translation="MMGSDNGNSDEKPVHRVELEGFCLDITPVTVDAYARCRGCSTPG
                     SGEFCTWRRARKGNHPINCVDWGQASAYCRSVGKQLPTEAQWEYAARGGSRQLEYSWG
                     SEGPQGRACWNRQEGTCAVGSYASGAFGLKDMAGNVWEWVQGWYGDYPSSVSRNHAGA
                     FSDSSRVCRGGCWVINAPTGLRGAYRLDVASGNRSSGLGFRCARTR"
     gene            2851..2997
                     /locus_tag="PK710_22455"
     CDS             2851..2997
                     /locus_tag="PK710_22455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12559.1"
                     /translation="MKDPEGIHAMPLGQFDPCDGGDTGGVAVGPGSDTAGGGADAVRS
                     GLSS"
BASE COUNT          716 a         1085 c         1002 g          679 t
ORIGIN      
        1 aggccgcttg cggccgtccg gagattgttg gtcggatggt gcttgacaag cgcatgtatg
       61 ctccgccgtg gagccgcgtg ctgcgcctgc gacacagcag cggcagccta tccgagagat
      121 gctgcccgct ccggctaatt gctcgacaaa tcgcattgct ttgggcactt cccggcaacc
      181 ggagcgcaga agcattcctg ataccgaacc gtccaggttc gaatttccag ctcccgaacc
      241 ccgttctcgt ccatcttttc ggtgcgaaac ccagcgttgg caggcaaggg aagccgaccg
      301 taggaaccgt acaacctcac gctttgggac tgccaacacc cgtggttcga aacgtagtag
      361 gaccagtacg tttgcggcgg gcagcacagc tcctccccag ttcgcgagac gaagtccgtc
      421 acgccgtcgc agtccaagtc tcctaccgga agaaggcttc cggtcggcgc cgttgtcgta
      481 cacgtttgcg ctgtagtggg cgggggtccc gcgatgctgt ccgcatcgaa cgccaaagcc
      541 atcactcttt cgtcgtacga tgagccgttc cacacgaaca ctgtgccatc agcgagcagg
      601 atgtccttgg cgcgatgacg aggggacgtt agcaaaacgt ggctggccaa acccggcttg
      661 ccgtcgtagc ccgactcgac cgtagccacg cttcgccagc acgcgccgcg tctcacacgg
      721 atggtttcaa tgatgatcgg aatgaggttc cttacgacag ttacgtgtac cgcgtctgga
      781 ataccgtctc cgtcgagatc cgggaggggg cgccaattct ctgggtcgtc gtcgcgggat
      841 atacaatcgt tcgctgggtc aggcccgcgt cgaggctcga actgcagccc gtaaggttcg
      901 aaatcgaatc gtacggttcc ccccgtcgct tgccctcccc gcccggcttc tgcaccacga
      961 gccacgatgg tgcgaagcgg acgggcaggt ccttcgacag agagcgatgt tgccaagata
     1021 tggcccaagt acgcccaaca actgggtctt ttcaaaagga tgtggtgaac agccttgcta
     1081 gtctggcatc cccgacggac actgatgatc cgatcgggcg ttccgtcgcc gtcgatgtcg
     1141 atattgttcc gagtatccac gcgtgcgccc ggcaccgctc gaattctttt tacccaatcg
     1201 tccggttccg gtccgcagcg aaggcggtcg acccgagcca tttcggtccc aggcgccggc
     1261 acaagggcat cgacatcggt atcagcatcg acatcagcat cggctgcgct gacagccgag
     1321 gtgggaatca ccgaggaagt cgttggcgac gggctttggg cgtctcccgg ccttccgatc
     1381 gaaagcagac cagcacccac ccccagtatc accaataagc tcacgatgtg ccgacggaga
     1441 cggccacttc ctgatcttgc atatttctcg ttgtacatgt cgacgcgcac ctcagcagcc
     1501 acacacgagc gtcttgtttc cagcggatcc gtttgtcctg cgttcgcctc gatccgtctc
     1561 tccgctcgcc ggtgcatcga cgaatttgcg gcaaatcggg atcttagcga tgccgttgag
     1621 cacattccaa gccaggggag tgttcggctc gacaacccgg cgcacttccg ggtcagccga
     1681 cgggaaggct tggagcttca cgagcgatgg aaccacgatc ggggtgaagc gcacaagctc
     1741 gcacaatccg acagtgctga gcaaggcagc gaagagggca aaatggttct tgcgcatgac
     1801 ttgggaccct gccaccgagt aggcaaccgc catgccacgc ggcgttcgag ggaaatgcac
     1861 caggggtcgc gggtggagat gccggtggtg tccaaggcgt tcacggtggt gtccgacgac
     1921 ggacaccgaa tctccggcac ctttgtgcgg gaacctcacc ttaacccgca cgatccgtaa
     1981 gtctgcgaaa ttccaaatct atgtacagcg acgttgatac aactcagccc gacggccccc
     2041 tccgcgggtc gatacaacag ggtgagcgag gcaaggaggc cggggcgaag catgataggc
     2101 gtggtatcac gaacaacgac ggtttggggg gggcgatagg gcgaggatgg ctggtgggtg
     2161 cgagggaacg cggggatacg aattgagggt tctgctgtcc tacagaaaat agcaagactt
     2221 acaccgcctt cctacctggt cctggcgcaa cgaaacccga ggccgctgct cctattgccg
     2281 gaagcgacgt cgagccgata ggcaccgcgc agccccgtcg gggcattgat gacccaacag
     2341 ccgccgcggc acacgcggct agagtccgaa aaagccccgg catgattcct acttactgag
     2401 cttgggtagt ccccgtacca gccttgcacc cattcccata cgttccctgc catatccttc
     2461 aatccaaacg cccccgaggc atagctgccc accgcacacg tcccttcctg acgattccaa
     2521 cacgctcgcc cttgggggcc ctccgaaccc cacgaatact cgagctgcct cgatccgccc
     2581 cgcgccgcat actcccactg ggcctccgtg ggcaattgct tgcccaccga acgacaatag
     2641 gcactcgcct gcccccaatc tacacaattg atcggatgat tcccctttcg cgcccttctc
     2701 caggtgcaga attcgccact acccggcgtc gaacacccac ggcaacgcgc gtacgcatcc
     2761 accgtcacag gcgtgatatc cagacaaaat ccctccagct ccacacgatg caccggtttt
     2821 tcgtcactat taccgttgtc ggagcccatc atgaaagatc ctgaagggat ccacgccatg
     2881 ccactggggc aattcgatcc ttgcgacggt ggagataccg gtggtgttgc cgtgggaccc
     2941 ggttccgaca cggctggcgg tggtgcagac gccgtgcgaa gcggcttgag ttcctgacga
     3001 agcggttcct tcggtttttc ctcgcccaca tccaaagggc gtgagaccac gttcggagga
     3061 gtacacgcag acaggaccac aagcggaagc catcgcacga acttcatgca ttgagaatat
     3121 cgggagagaa agctggcgca caagaggaag cggccatggg gataaagata tttggatggg
     3181 atgggcggct tttgtttttg ttctgtcgcc cttgtcccca catagtagcc tcccattccc
     3241 ggcccttccc cccacaccgt gggcccccca tcccctgtcc ttccccccac accgtgggtg
     3301 gaagggagat ataatatcta attataattc cttgttccta gtttctagct tataaacatt
     3361 ttagctaata attaacaact gttttaattc ccctccaccc cgattcgggg gggagggcca
     3421 gggtggggga aataaacggg ggcgagggcc agggtggggg gaaataaacg ggggcgaggg
     3481 cc
//
LOCUS       DAOSOR010000611         3474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_27186_length_3474_cov_3.144487, whole genome shotgun sequence.
ACCESSION   DAOSOR010000611 DAOSOR010000000
VERSION     DAOSOR010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27186_length_3474_cov_3.144487"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PK710_22460"
     CDS             complement(<1..657)
                     /locus_tag="PK710_22460"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT12560.1"
                     /translation="MRSNGVLLVPIDLSPGSQTALQAARFLATAYQARLVVMHVVDKA
                     PAIGALRWEAGRNADVSTADAEAEVQQWLATRGFECQDIVVVVGMPGAEVIRKAKELA
                     VDAIVMATHGVTGIQARFLGGTAYQVLRGVRCPVMTVKPPGFGALLFKMWEGIQLFGE
                     KLPPFHDGSVGLPPKHVLHPTDFSDASHQATLLAARMAGHLDAQLTLLHVCPDTDTVQ
                     D"
     gene            complement(662..1486)
                     /locus_tag="PK710_22465"
     CDS             complement(662..1486)
                     /locus_tag="PK710_22465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12561.1"
                     /translation="MTHFSEQKVVLETGTPMVCLCPHCRASLVQGEHVLLEVERHDQQ
                     SGHLRLSPRLNVFDSTSTIELVEGVEVADLRCADCKTTLGDSQRRCTLCGSRVARLWI
                     NIDGQDVDFYICMRKGCRWHAISQAARSRLILQTAGFHKPDRPSELIQSGTKLDCSCP
                     YCDHDLVQGDDLVVRITNAPQEVGTLILSPYLNDFRASSSIPAEKGVVAEDFACPFCL
                     RSLKDPHRRCNLCAASTAVFNVRTSAGSAHIYVCMRRQCHWHYLDDEQRAWALEPD"
     gene            1624..2484
                     /locus_tag="PK710_22470"
     CDS             1624..2484
                     /locus_tag="PK710_22470"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT12562.1"
                     /translation="MKFPRWVWLAINFWAFWGCTPSEEPAPTVIEVPEASPVPSAVPS
                     PSPPAACPPGMVEVKGEYCPTVMHQCIEGGKNHDGKPSSDPMPYYCEAYRPGYAKCMK
                     KEVPQHFCIDIYEYPNEKGVLPEVFVSWYEAKALCEKIGKRLCDDDEWTLACEGPERL
                     PYPYGWRRDRGRCNIDKPWRKPNDGILARKTRDGKVEAEIERLSQRHVSGAMPGCVSA
                     YGVMDMGGNVDEWTVNVTLGGKPYRSMFKGGHWCGGARNRCRPTTESHDETTRYYAEG
                     FRCCASPRAN"
     gene            2788..>3474
                     /locus_tag="PK710_22475"
     CDS             2788..>3474
                     /locus_tag="PK710_22475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12563.1"
                     /translation="MRTLIIALATVIVVLLMAPRAQAIESLTAQDVVSWAQYAVGYSY
                     RWGHASWRTDKALPGRCSGTCPRCQNSGPNGADCSGFLAKVWRVPSSIPMTTDSPNVY
                     STLHFYSTNLHWSRIDRADVKQADAMVYRAKNKKGQDVGHIFLYDSKDPWGIMWTYEC
                     LSCKAGCVHHLRTAPSPYQAIRRNSMVTKAKSCTYHHECSTGNCAWNGDIYCCRDPGF
                     TGETCYHDHDP"
BASE COUNT          778 a          887 c         1059 g          750 t
ORIGIN      
        1 atcctgcacg gtatcggtgt cggggcaaac gtgcaggagc gtgagttggg catcgaggtg
       61 tccagccatc cgtgcggcaa gcaaggtggc ttgatgagag gcgtcggaaa agtcagtggg
      121 atgcagcacg tgttttggcg gcagacctac ggagccatca tgaaaaggcg gcaatttttc
      181 gccaaacagt tgaattcctt cccacatttt gaatagtagg gctccaaatc caggtggttt
      241 gaccgtcatg accgggcaac gaacgcctcg aagcacctgg taggccgtac ctcccaaaaa
      301 gcgggcttga atccccgtga caccatgagt tgccatcacg atggcatcga cggcgagttc
      361 tttggcctta cgaatgactt ccgcgccggg catccccaca acgaccacga tatcctggca
      421 ttcgaatccg cgcgtcgcca gccattgttg gacttccgct tccgcatcgg ctgtcgagac
      481 atcggcgtta cgccccgctt cccatcgcaa tgcgccaatg gcgggggcct tgtcgacaac
      541 atgcatgacc acgagacgcg cctgataggc tgtggccaag aaccgagcgg cttgcaacgc
      601 tgtctgggag cctggggaaa ggtcgatggg gacgagaaga acaccgttgg aacgcatcga
      661 ttcaatccgg ttcaagcgcc caagcgcgct gttcgtcgtc caaataatgc caatgacatt
      721 gtcggcgcat acagacgtag atatgggcgc ttccggcgga ggtgcgtaca ttgaacacgg
      781 cggtggaggc cgcacagaga ttgcagcgtc gatgagggtc tttcaggctt cgaaggcaga
      841 aggggcaagc gaagtcctcg gcgacgacgc ctttttccgc aggtatggaa ctcgaggccc
      901 gaaagtcgtt gaggtagggg ctgagaatga gcgtgccgac ctcttgtggg gcgttggtga
      961 tacggaccac caagtcatcg ccttgaacca ggtcgtggtc gcaatagggg caggagcaat
     1021 cgagtttcgt tccggactga atcaattcgc tggggcggtc tggtttgtgg aatccggccg
     1081 tttgcaggat caatcgactt cgagcggctt ggctgatggc atgccaacga cagcctttgc
     1141 gcatgcagat atagaagtcc acgtcttgtc catcgatgtt gatccagagg cgtgcgacgc
     1201 gcgagccaca cagggtgcat cgtcgttggc tgtcaccgag cgttgttttg caatccgcac
     1261 agcgcaggtc cgccacctcg accccttcga ccaattcgat ggtgctggtg ctatcgaaga
     1321 cgttcagccg ggggctgagc cgcagatgtc cgctttgctg gtcgtgtcgt tccacctcga
     1381 gaagaacatg ctcgccctgc actagcgaag cccggcaatg ggggcagaga caaaccatgg
     1441 gcgtgcccgt ttcaagcacg accttttgtt ccgaaaagtg ggtcatgaaa gatatccttg
     1501 ctacgaatgg gcatgttgac aaggccgagg cacgatcgca agcatggatg ttcgatggtt
     1561 gtctttcacg actgccgata tttaggggcg gcatcgtgct gccccttcga ccgcgcaagg
     1621 ttcatgaaat ttccgcgttg ggtatggctt gccatcaatt tttgggcttt ttggggatgt
     1681 actccttcag aagagccggc tcctacggtt atcgaagtgc ccgaggcatc gcccgtgcca
     1741 agcgcggtcc cctccccttc cccaccagcg gcttgtccac cggggatggt cgaagtcaag
     1801 ggggagtatt gtcccaccgt gatgcatcaa tgtatcgagg ggggaaaaaa tcacgacggc
     1861 aaaccgagca gcgatccaat gccctattat tgtgaggcgt atcgtcctgg ctacgcaaag
     1921 tgtatgaaaa aggaagttcc gcagcatttt tgcatcgata tttacgagta tccgaatgaa
     1981 aaaggggtgc tcccggaggt tttcgtgtcc tggtacgaag ccaaggcgtt atgcgaaaaa
     2041 atcggaaaac gtctgtgtga cgacgacgaa tggacgctgg cttgcgaggg accggaacga
     2101 cttccctacc cgtacggctg gcgtcgggac cgcggccgat gcaacatcga caagccctgg
     2161 cgcaagccga acgatgggat tttggcgaga aagacgcggg atgggaaagt cgaggcggaa
     2221 atcgagcgtc tgtcgcagcg acacgtttct ggcgcgatgc cgggatgcgt gtccgcgtac
     2281 ggcgtcatgg acatgggggg aaacgtggat gagtggacgg tcaacgtgac gttggggggc
     2341 aagccgtacc gttcgatgtt caaggggggg cattggtgtg gcggggcgcg caaccggtgt
     2401 cgaccgacca cggagagcca tgatgaaacc acgaggtatt acgcagaggg tttccggtgc
     2461 tgtgcttctc cacgggcaaa ctaaccattt gtttttatta gagtaaatga ctgttccagg
     2521 aacgtaatcg taagacgctg aggccgtgtg gatccaaggg aaatcgaagg tgggagtata
     2581 ttttcggcac tggcaaatcg gtagagtatc ggcttcgacg tcactatcgc tgatgagaaa
     2641 cgtgaatggc gccgtgtgta tgaaccgtgc gcatagggaa gaagtcgcac tcacgaactg
     2701 tggccaatca gtggctcgag acccatttca ccagcgcttg cggcaaaatt ccgcttgggt
     2761 aagaaaattg catgtttcat catcacgatg agaactctga ttatcgctct cgcaacggtc
     2821 atcgtcgttt tgctcatggc accccgggca caggccattg aatcgctgac ggcgcaagac
     2881 gtggtgagtt gggcacaata tgccgtgggt tattcttatc ggtgggggca cgctagctgg
     2941 cgaacggata aagctttacc ggggcgttgt tcgggaacct gtcccagatg ccagaacagc
     3001 gggcccaatg gagcggattg ttccggattc ttggcaaaag tgtggcgagt accgtcgtcc
     3061 atacccatga ccacggattc tcctaacgtc tattcgacct tgcactttta ttctaccaat
     3121 ctgcattggt cacgaatcga tcgtgcggac gtgaaacaag cggatgcgat ggtgtaccgc
     3181 gccaagaaca agaagggtca agacgttggg cacattttct tatatgacag caaagacccc
     3241 tgggggatca tgtggacgta cgagtgtttg agttgtaagg cgggctgcgt tcaccatttg
     3301 cgaaccgctc cgtccccata ccaagcgatc cgtcgcaact cgatggtaac aaaggccaaa
     3361 tcctgcacat atcatcacga gtgttctacg gggaattgtg catggaacgg tgatatctat
     3421 tgttgtcgcg acccaggttt tacgggagag acatgttacc atgaccatga cccc
//
LOCUS       DAOSOR010000612         3464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_27278_length_3464_cov_3.808448, whole genome shotgun sequence.
ACCESSION   DAOSOR010000612 DAOSOR010000000
VERSION     DAOSOR010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27278_length_3464_cov_3.808448"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PK710_22480"
     CDS             complement(<1..374)
                     /locus_tag="PK710_22480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12564.1"
                     /translation="MMQPPSPPLPEVNFEHLRLLSIFYYVLGAIYVMVALIPLIHLSV
                     GIAIVTGVLHSPPDTIPLGWMFIGLAAVVVIVGQTYAICMLLTGRYLAAHKAWKFCYV
                     VAAVNCMNVPLGTVLGILSLIQL"
     gene            complement(371..1444)
                     /gene="mltG"
                     /locus_tag="PK710_22485"
     CDS             complement(371..1444)
                     /gene="mltG"
                     /locus_tag="PK710_22485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOT12565.1"
                     /translation="MVRSKWFKAALATVSSLMILSMGFVAFLLLPCHGDGSSVEVEWD
                     APESAIEAADLLARAGLVRSVWLMALYLRVDGHWDRIDSGIHLLQNDMAPRTLLRRLR
                     RLQGGAEVRVIIPEGFDTFDVARRLQESGVCSQRALLKAAHDATLMAELRLPVKDAEG
                     YLFPATYAFVRNQSPSSIVRRMVNEAIKRHAGLFDEHPEALKTLHDDLGWNRHDILIL
                     ASMVEKEAAVDEERPLIASVFLNRMRSASFRPKQRLQSDPTARYGCLLDPLKIPTCAG
                     AEKGVTGPMVRDPANPYSTYAHAGLPPGPICNPGERSLLAVLTPAITNYLFFVARGNG
                     RHSFSESFESHRDAIRDAQSNPR"
     gene            complement(1612..2058)
                     /gene="ruvX"
                     /locus_tag="PK710_22490"
     CDS             complement(1612..2058)
                     /gene="ruvX"
                     /locus_tag="PK710_22490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOT12566.1"
                     /translation="MIVVDPPIMRVAALDLGKVRVGLAVTDEIGAMAHPRPPLDARVR
                     SDLLRKIKDFAEQESVDHFVVGLPLDPAGQRGPAARKAMAFAQQLFEFTGINVVLWDE
                     RFSTSEATRRLREGGHRARESRAKIDSAAACLILQAWLDSQSGRAR"
     gene            2154..3443
                     /gene="hisS"
                     /locus_tag="PK710_22495"
     CDS             2154..3443
                     /gene="hisS"
                     /locus_tag="PK710_22495"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOT12567.1"
                     /translation="MNYRAVKGMNDILPDEMVRWHHLEREFRVVAERHGYGEVRTPII
                     ESTALFTRSIGEATDIVEKEMYSFERHGEALTVRPEGTASAVRAYVEHHIHQKEPVTR
                     WYYLGPMFRGERPARGRYRQFHQLGCELFGDAGSVCDAEMIDMLHGLFMRLGISDLEI
                     VINSLGGADTRPRYRQIIVDHLEPRKARLSEDSIRRLSSNPLRILDSKDERDIAALEG
                     LPSIIEVLTQDDREHFDRLCSQLDRLGTPYRVDPTLVRGLDYYTRTLFELRSQAGQVG
                     AQNALGGGGRYDNLVQQMGGPSTPAIGFALGVERILLAMDVAELASPPLVVIAPIGPQ
                     AASEALLMARELRDHGICVELDGRGGASTSLKAMLRRANARGARVCLVLGQEELESQK
                     VQLKDLAARSQVLVAREELMHRLLDVLQQPMMTGGAA"
BASE COUNT          777 a          936 c         1042 g          709 t
ORIGIN      
        1 agctgaatca acgacaaaat tccaagtacg gtgcctaacg gcacattcat gcaattgacc
       61 gctgcgacaa cgtaacaaaa cttccaagcc ttgtgtgcgg ccaggtagcg ccctgtcaac
      121 agcatgcaga tggcatacgt ttgtccgacg atgacgacta cggcagccag gccgatgaac
      181 atccaaccga gagggatggt gtcaggtggt gagtgaagca cacccgttac gatcgcaata
      241 ccgacgctca gatgaattaa aggtatgaga gcgaccatga cgtagatggc acccaggaca
      301 taataaaaaa tggaaagcag ccgaaggtgc tcgaagttga cttcaggcaa gggaggagaa
      361 ggcggttgca tcatcgtgga ttggattgcg cgtcgcggat tgcgtcgcgg tgggattcga
      421 acgattcgct gaacgagtgt ctgccattac ctctggcaac gaaaaaaaga tagttggtaa
      481 tggccggggt gagaacggcg agcaaggagc gttctcctgg attgcagatg ggtccgggcg
      541 gaagtcccgc gtgggcgtag gtgctgtaag ggttggctgg gtctcgcacc atgggccccg
      601 tgacaccttt ttccgcgccg gcacaggtgg gaattttcaa agggtccagc aaacaaccgt
      661 atcgagccgt aggatcgctt tgtagccgtt gtttcggtcg aaaactcgca ctgcgcatcc
      721 gattcaaaaa cacgctggcg atcaatggtc gttcttcgtc caccgcagcc tctttttcga
      781 ccatcgacgc aagaatgaga atgtcatgac ggttccatcc caggtcgtcg tgtagggttt
      841 tcaacgcctc gggatgctca tcgaacagcc ccgcgtggcg tttgatcgcc tcattgacca
      901 tgcgacgcac gatggacgac ggggattgat ttcgtacgaa cgcgtaggtt gcgggaaata
      961 agtagccttc ggcatctttg acgggcaggc gcaattcggc catcaaggtg gcatcgtgag
     1021 cggctttgag caacgcgcgc tggctgcaca cgccgctttc ctgaaggcgg cgtgccacat
     1081 cgaacgtatc gaatccttcc ggaatgatga cacgaacctc agcgccgccc tgcaatcggc
     1141 gcaagcgtcg caacaaagtc cgcggtgcca tgtcgttttg caacaaatgg atgccggaat
     1201 cgattcgatc ccaatgtcca tccacgcgta gataaagtgc catgagccaa acggatcgca
     1261 ccaagcctgc acgagcgagc aggtcggcgg cttcgatggc ggattccgga gcgtcccatt
     1321 cgacttccac cgaagaacca tcgccatggc aaggaagcaa gaggaacgca acgaagccca
     1381 tgctcagaat catcagcgaa gagacagtgg ccaaggcggc tttgaaccac ttcgaacgaa
     1441 ccacggtccg ccggcgggct ggggtatcag cgcgtttgtt ttcgcgatgg ggttgaggta
     1501 gccgggaatt tttcgatggc cgggattggg aatgctttgg gcttccggag cctttgggcg
     1561 agggggtgga ccgtggcttt cgcttgggtc gaggttcttt cgcgcttccg ctcaccgtgc
     1621 tctcccagac tgactatcga gccatgcctg tagaatgaga caggccgcgg cactatcgat
     1681 ttttgcacgg gactcacggg cgcgatgccc accttctcga agtcgtcgag tggcctcgga
     1741 agtcgagaag cgttcatccc aaagaacgac attaatacct gtaaattcaa acagttgctg
     1801 agcaaaggcc atcgcttttc gcgccgcggg tccgcgttgt ccagccgggt ccaaaggcag
     1861 tcccaccacg aagtggtcca ccgattcttg ttcagcaaag tctttgattt ttcgcaacag
     1921 atcgctacgg acccgggcat cgaggggcgg acgcgggtgc gccatggcgc cgatttcatc
     1981 ggtgacggcc aatccaacgc gcactttgcc caagtcgagg gccgcaacac gcataatcgg
     2041 gggatctacc acaatcatgg ggctcgagaa tgacgatatg cgccgggtag atcacgttca
     2101 cgttgttcct tgcttgaccc gtccgcttgc accgtccata agccgcccag tcaatgaact
     2161 accgagccgt caaagggatg aacgacatcc ttccggacga gatggtccgc tggcatcatc
     2221 tcgagcgaga attccgcgtt gtggcggagc gtcatggtta cggtgaggtg cgaacaccca
     2281 tcatcgaatc caccgccttg ttcacacgct ccatcggaga agcgacggat atcgttgaaa
     2341 aggagatgta ttcgttcgag cgccacggcg aagcgttgac ggtgcggccg gaagggacag
     2401 cgagcgcggt ccgagcctat gtggagcacc acatccatca aaaggaaccg gtcacgcgct
     2461 ggtattactt ggggccgatg tttcgcggag agcgccccgc gcgaggacgg taccgtcagt
     2521 tccatcaact cgggtgtgaa ttatttggtg acgcgggttc tgtatgcgac gccgaaatga
     2581 tcgatatgtt gcacggatta ttcatgaggc tcggcatctc ggacctcgag atcgtcatca
     2641 attctctggg gggcgccgat actcgtcccc gttatcgcca gatcatcgtg gaccatctcg
     2701 agccacgaaa agcacggttg agcgaggatt cgatccgacg tctgtcatcc aatcctcttc
     2761 gcatccttga ctcgaaggac gaacgagaca ttgcggcact cgagggattg ccatcgatca
     2821 tcgaagtgtt gacgcaagac gaccgggagc atttcgacag gctgtgctcc caactcgacc
     2881 gtctcggcac tccgtatcgc gtggatccga cgttggttcg ggggctcgat tattacacgc
     2941 gaaccctatt cgagctacga agccaggcgg gtcaagtcgg agcgcaaaac gcgctggggg
     3001 gaggaggacg atacgacaat ctcgtgcaac agatgggagg gccgtccaca ccagcaatcg
     3061 gatttgcgct tggcgtggaa cgtatcctgt tggcgatgga cgttgccgaa ctagcctctc
     3121 cacctttggt ggtgatcgct ccgatcggtc cgcaggcggc gtcagaagcg ttgctcatgg
     3181 cgcgagaact ccgagatcat ggcatttgcg tcgaactcga tggtcgaggg ggcgcatcga
     3241 cttcgctcaa ggccatgtta cgacgcgcga atgctcgcgg cgcgcgggtg tgtttggtgc
     3301 ttggacagga agagttggag tcgcaaaagg tacaactcaa agatctcgct gctcgttcgc
     3361 aagttcttgt ggcaagagaa gaactcatgc atcggctgct cgacgtattg caacagccca
     3421 tgatgaccgg aggcgcagct tgacgcccaa acggaatctc aggt
//
LOCUS       DAOSOR010000613         3458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_27353_length_3458_cov_3.360270, whole genome shotgun sequence.
ACCESSION   DAOSOR010000613 DAOSOR010000000
VERSION     DAOSOR010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27353_length_3458_cov_3.360270"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3458)
                     /locus_tag="PK710_22500"
     CDS             complement(<1..>3458)
                     /locus_tag="PK710_22500"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOT12568.1"
                     /translation="VYAYILRKTLGEACAEAGECETGHCVDGACCAQSACPDCHSCAA
                     PGSQGQCTAMPSLQGKSCGDSSTSECSQPDTCDDQGVCQPNHVAEGTACGSHEATACT
                     EPDSCDGLGTCLPNHRPEGYECGDNTKNECTDPDTCNGEGACVPNHQPDGTACSGGVC
                     DQGQCSGGGTGGAGGASGSGGSGAEAGQGGSGASAGTSGSAGSGGAKTPPAADSDSDD
                     GCGCRMTRHAAPNGFLVVLLMAMAVIARRNGSFPWRKLLASGLLVPVVSATACGNETD
                     IVVPPDANGQGGSSADGGTDGAVGGQSGTGASAGVGGSGAYGGGTVCPPEKQCGLACC
                     TDNQECIDNQCFEKCPAPRERCGDPPVCCEADQQCHKGQCIAACTPPTVPCGQETIVC
                     CDATDVCFNNQCVVPGGDCIDFADCDDDEYCEPTLNRCLPATALPACEYHPPVGEFQP
                     AREWAYSPVTAPYVRASSSSPAVDICSQGFPLAFANSDEALAVVPLGGLILPFFDKDR
                     TAMIVSTNGWLTLDANYQGGPAPQNTELLSPSTPSLVVAPYWDDLKNVTACASVNTSV
                     GSVAVQWRGQVGNTGEWIEFQANLHAKNHADPGVIELKYAQLGSTQDGDGATIGIAEV
                     STGRAIQHGFNRNGAVNTSTSLRFHYVPLRNRFDALAGPAVFDLDGDGKPEVILAAYD
                     DTPTSSGVPNDPIYDMHSGVLTILNGEDGTIQARPFETPGYAGYLGAGIALSVGDLDG
                     DGKVEIVGVGRLEPMVDSKPYVKAFNADGTLKWISNTDVGFNHNGWGGGVHIADLDGD
                     GRAEIFFGLSVFNHEGMLLWSKPVEYGSPATTAADLDGDGDLEIVSDRSAWHHDGTPL
                     WQRPDLGAYHFPSIADFDLDGKPEVALVANGSLIILNGHDGSTFWGPLSLQTDGGGPL
                     NIGDFDGDGYPEIGTAGEGLYLVIDLQCEGSPLPAGCDRTGVRWSSPTKDISSNVTGS
                     SLFDFEGDGVAEVVYSDECFTRVYSGTSGKVLFETSVNTRTATEYPLVADVDGDNNAE
                     IIVVANENVVGCSTAPWSIGLTGAPWKPADYPAPFCTTSLCGHRGIAVYGDALDNWVR
                     TRRVWSGHAYHITNVLSSGAIPQHEERNWTNPRYNNFRMNAQGAALFSAPDLQVQLDV
                     DPLVCP"
BASE COUNT          703 a         1017 c         1030 g          708 t
ORIGIN      
        1 ggggcagacg aggggatcga cgtcgagttg cacttgcaag tccggagcgc tgaacagcgc
       61 ggcaccctgg gcattcatgc ggaaattgtt gtagcgggga ttggtccaat tgcgttcttc
      121 gtgttgcggg atggcaccgc tcgaaagcac attggtgatg tgataggcgt gtccactcca
      181 tacgcgtcgc gtgcgcaccc aattgtcgag agcatcgccg tatacggcga taccgcgatg
      241 gccgcacagg gaggtcgtgc agaaaggagc ggggtaatcc gcgggtttcc agggggcgcc
      301 ggttagtccg atgctccacg gggcggtgct gcagccgacg acgttttcgt tggcgacaac
      361 gatgatctcg gcgttgttgt cgccatcgac gtcggcgacc aagggatatt ccgtggcggt
      421 gcgtgtgttg acggaggttt cgaaaagcac cttgccactg gtgccagaat acacgcgggt
      481 gaagcattca tcggaataca cgacttcggc gactccatcg ccttcgaagt cgaacaagga
      541 agagccagtg acattggagg agatatcttt ggtgggagaa ctccatcgga cacccgttcg
      601 atcgcaaccg gcgggcagag gggagccttc gcattgcaag tcgatcacga ggtacaatcc
      661 ttcgcccgcg gtgccgattt cgggatagcc atcgccatcg aagtcaccga tgttcagagg
      721 cccgccgcca tcggtttgca aagacagcgg cccccagaag gtgctgccgt cgtgaccgtt
      781 gaggatgatg agcgaaccgt tagcgaccag ggcaacctct ggttttccat cgagatcgaa
      841 gtccgcgatg ctgggaaagt ggtatgcgcc caggtctgga cgctgccata acggggtgcc
      901 atcgtgatgc caggcgctgc gatcggaaac gatttccagg tcaccatccc catcgaggtc
      961 tgcggcggtg gtggctggac ttccatactc caccggtttg ctccacagca gcatgccttc
     1021 atgattgaac acgctcagac caaaaaagat ctccgcgcgc ccatcgccat ccaagtcggc
     1081 aatatgaacg ccgccccccc aaccgttatg attgaatcca acgtcggtgt tggatatcca
     1141 cttcagggtc ccatcggcgt tgaatgcctt gacataaggt ttcgaatcca ccatgggttc
     1201 gagccttccc acgccgacga tttccacctt gccatcacca tcgaggtcgc ccacgctcag
     1261 ggcgatacca gcgccgaggt accctgcata accaggggtt tcgaagggac gagcctgaat
     1321 ggtgccgtct tctccgttga ggatcgtcag cacgccgcta tgcatatcgt agatgggatc
     1381 gttggggacg ccggaactgg tcggggtatc gtcataggcg gcaaggatca cctctggttt
     1441 gccatcccca tcgagatcga agacggcggg acctgccaga gcatcgaagc gattgcgcaa
     1501 cggcacgtag tgaaaacgca gggatgtgct tgtgttcacc gcaccatttc gattgaagcc
     1561 gtgttggatg gctcgtccgg tggacacctc ggcgataccg atggtggccc catcaccgtc
     1621 ctgcgttgaa ccaagttgtg cgtatttgag ttcgatgact cccgggtccg catggttttt
     1681 ggcatgcaaa ttcgcctgaa actctatcca ttctcccgtg ttgcccacct gtccgcgcca
     1741 ttgcacggct acgcttccca ccgaggtatt cacggaagcg caagccgtca cgtttttcag
     1801 gtcatcccaa tagggggcca cgacgaggct gggggtagag gggcttaaca attcggtatt
     1861 ttgtggagca ggtccgccct ggtagttcgc atcgagcgtc aaccaaccgt tggtggaaac
     1921 aatcatcgcg gttcggtctt tgtcgaaaaa gggcaagatc agccctccga gaggaacgac
     1981 ggccaacgct tcatcggagt tggcgaaagc gagaggaaat ccttgtgagc agatgtcgac
     2041 ggcaggagaa gaggaggatg ctctcacata cggagcggtg accggcgaat aggcccattc
     2101 tctggcaggt tggaattcgc ccaccggagg atgatattca caggcgggca aggcagtcgc
     2161 gggaaggcat cgattcagcg tgggctcaca atattcgtcg tcatcgcaat ccgcgaagtc
     2221 gatacaatcc ccgcctggaa cgacgcattg gttgttgaag cacacatcgg tggcatcgca
     2281 acatacgatt gtctcctgac cacaggggac cgtgggtggc gtacaggcgg caatgcattg
     2341 acctttgtgg cattgctgat cggcttcaca gcatacgggg ggatctccgc aacgctctcg
     2401 gggagcgggg cacttttcga agcattggtt atcgatgcat tcctggttat cggtacagca
     2461 cgcgaggcca cattgttttt ctggggggca gacggttcct ccaccataag cacctgagcc
     2521 tccaacgccc gcggaggcgc ccgtaccgga ttgtcctccg acggcgccgt cggttccgcc
     2581 atccgcgctc gaacctccct gaccattggc gtcgggaggc acgacgatat ccgtttcgtt
     2641 gccgcatgcc gtggcggaga caacaggtac cagcaagcca gaggcaagaa gcttgcgcca
     2701 tggaaacgag ccatttcgtc gggcgatcac ggccatcgcc atcagcaaaa ccacgagaaa
     2761 accgttgggg gcagcatgcc gagtcattcg gcagccgcat ccgtcatcgg agtctgaatc
     2821 ggcagcgggg ggagttttcg cgccaccgct gcccgcgctg cccgaagttc cagcgcttgc
     2881 tcccgagccg ccttgtccgg cctcggctcc cgagcctccc gagcccgacg ctcccccggc
     2941 gcctcctgtt ccccctccag agcactgtcc ttgatcacac acgcctccgg agcaggcggt
     3001 accatccggt tgatgattgg gaacacatgc tccctcccca ttgcatgtat ccggatcggt
     3061 acattcattc ttggtgttgt cgccacattc atagccttct ggacgatgat tcggcaaaca
     3121 agtcccgagc ccatcacagc tatccggttc cgtacacgcg gtagcttcgt ggctaccaca
     3181 agccgtccct tcggcaacgt gattgggctg gcatacgccc tggtcatcgc aggtatccgg
     3241 ttgggagcac tccgaagtcg acgagtctcc gcaggatttt ccttggagcg atggcatggc
     3301 cgtgcattgt ccttgcgaac ctggagcggc acaggaatgg caatccggac aagcgctctg
     3361 ggcacaacac gcgccgtcga cacaatgacc ggtctcgcat tcccccgcct ccgcacacgc
     3421 ttcccccagc gtttttcgca agatataggc gtatacgg
//
LOCUS       DAOSOR010000614         3455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_27381_length_3455_cov_3.584412, whole genome shotgun sequence.
ACCESSION   DAOSOR010000614 DAOSOR010000000
VERSION     DAOSOR010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27381_length_3455_cov_3.584412"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1538
                     /locus_tag="PK710_22505"
     CDS             <1..1538
                     /locus_tag="PK710_22505"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HOT12569.1"
                     /translation="AGGSGGSGGSELDDWQCEAAPGGTEIYLSLNGDDTNAGTLNAPY
                     ATFGRAIQAAKAGDVIYVRAGTYGKKQAMVRGVARDEASYPATKCPDGEVLADKYCYH
                     DTYAFIALQDFSGWASNTPSYTVESGTKDHPIAICPYPGEHVTLDATGWTMRAVTVGL
                     KAHWIIAHFEIIGGMVNIGGGNANAQPHDIVIRGNNVHDITIEGGDNPGIVRIDRGDV
                     YGPWNIFVWGNELHDLYDIAQPGQWQNVQDAQHFGAVTTLSRQTYSGFDGGGTGYIEI
                     IGNTMYNLPQAFFFKNPMEGPIEIRHNIIRNAGSLGLMMAANVHMVHNLVSNVSDGWQ
                     AGNGGYDDDRITAISGQNAVIEYNTFVGLDSMLQLRCATGHQVQHNIIAGLSGSTKGA
                     NWDTPSFVSKQGIYPDPLDPAHSILQNMTSNHNCFISPHADFQLAARYLPQEVTGNGW
                     LVEHYDHAHAKTTFGFDIDSVTVVEANPSSLFLDPDAGDFQLIDPNRCPNMGYHAYPG
                     DAE"
     gene            2097..3254
                     /locus_tag="PK710_22510"
     CDS             2097..3254
                     /locus_tag="PK710_22510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12570.1"
                     /translation="MDPKASLGLMAIAALVVACSDDSSSNTPTGDSGSKTDSGAGGSG
                     GSNTDPGSGGSAGSGGSAGSGGSAGTGGSAGSGGSAGSGGSAGSPTDPGKAEIWEEGL
                     APGVNMGIAAQYPDDVGISSHPAVYAAEDFESGSVQLPTEEDRLVNNLTVVDNEVYTG
                     HYAAAFSWPEGFNGPTTRFALPADPNDNPHGAYFVRVCYNFDKSFHPGDQSVGVGVKG
                     FGIYAEGEGTTETTWYNTSVQFVGWGGSSKPQANDGYLWVGHLYSYNPHPDEAVATVG
                     ELDNVSEYRFSAYATPFDYIRFNTWRCYEVGLYLNTPGENDGEARFWIDGVLQSRVTH
                     MRFRDSITPRPDKVNISLHRVTENIPHTMTRWMDNIVIARRYIGPVKLSSP"
BASE COUNT          789 a         1157 c          859 g          650 t
ORIGIN      
        1 gtgccggcgg ctcgggcggc tcgggcggct cggaacttga cgactggcaa tgcgaggccg
       61 cgccaggcgg aaccgaaatc tacctctcgc tcaacggcga cgacaccaac gccggcaccc
      121 tcaacgctcc ttacgccacg ttcggtcgtg ccatccaagc cgccaaagcc ggagacgtca
      181 tctacgtccg cgcgggaacc tacgggaaaa aacaggccat ggttcgcggt gtcgcacgcg
      241 acgaagcctc ctatcctgcc acgaaatgcc ccgacggcga ggtgcttgcc gacaaatact
      301 gctaccacga tacctacgca ttcatcgccc tgcaggactt ttcgggttgg gcctcgaata
      361 ccccatccta caccgtagaa agcggcacca aagaccaccc catcgccatt tgcccctacc
      421 ccggcgagca tgtcacgctc gatgccacgg gctggaccat gcgcgctgtc acggtcggcc
      481 tcaaagccca ttggatcatc gcccatttcg aaatcatagg cggcatggtc aatatcggcg
      541 gcggaaacgc caacgcacag ccgcacgaca tcgtgatccg cggcaataac gtacacgata
      601 tcaccatcga gggtggcgac aacccaggca tcgtgcgcat cgaccgcggc gacgtctacg
      661 gcccgtggaa catcttcgta tggggcaacg aactgcacga cctttacgat attgcccagc
      721 caggccaatg gcaaaacgtg caagatgccc agcacttcgg tgccgtcacg acactctcgc
      781 gccaaaccta ttccggcttc gatggcggcg gaaccgggta catcgagatc atcggcaata
      841 cgatgtataa cttgccccag gccttcttct tcaaaaatcc gatggaaggg ccaatcgaga
      901 tccgccacaa catcatccgc aacgcgggaa gcctgggtct catgatggct gccaacgtcc
      961 acatggtcca caacctcgtc agtaacgtat ccgatggctg gcaagcgggt aacggcggct
     1021 acgacgacga ccgaataacc gccatctcgg gtcaaaacgc ggtcatcgaa tataacactt
     1081 tcgtcggcct cgactcgatg ctccaattgc gctgcgccac cggacaccaa gttcagcaca
     1141 atatcatcgc gggtctttcg ggcagcacga agggagccaa ctgggacacc ccttcgttcg
     1201 tgagcaagca gggcatctac ccggaccccc tcgaccccgc gcactccatc cttcaaaaca
     1261 tgacctcgaa ccacaactgc tttatctccc cgcacgctga ctttcagctt gcagcccgct
     1321 atctgccgca ggaggtgacc ggcaacggct ggcttgtcga gcattacgac cacgcccacg
     1381 cgaaaaccac gttcggattc gatatcgatt ccgtaacggt cgtcgaggcc aacccgtcca
     1441 gcctgttcct ggatcctgac gcgggagact tccaactcat cgatccaaac cgctgtccga
     1501 atatgggata ccacgcgtat ccgggtgatg ccgagtaagc tccataacga tacccccaaa
     1561 gctgccacgg ccgcggtcac ggtcacggtc acggtcacgg tcacggtcac ggcccacggc
     1621 gcaagccaca cccaaaagga ccacggtcac aatggcccac cgccatcccc gataagtccg
     1681 tgatgacggc cctctgccca cggtatcaca gctcgcctac tccccaactc cccatctcga
     1741 cccatcgtcc gatgaacatc gaaaagcgcc tatcaccagc ccaaataagg ccatgagagc
     1801 gaggtatcat gagactttca tttcaactat caagcgtatt aagaaatcac aaaccatccg
     1861 ccggtagctt cccaagctca ccataacatc ttgatattat tggtttaatt atcattgcac
     1921 ccgaatgcga gccccggctc accacgatcg cccacgaaaa aaaatacgat cgcacgtttt
     1981 ccttccctga tttctatcct cattcatcac ccgcctgttc ccagaaaaaa aacatggcgt
     2041 cactttggcc ccaatgatac gatagagcgt tctttccaag aattgggcaa aatcgcatgg
     2101 atccaaaagc atcgcttggt ctgatggcaa tcgctgcgct tgttgtcgcc tgcagcgatg
     2161 attcctcctc gaataccccc acgggcgaca gtggctcgaa aaccgattcc ggcgcggggg
     2221 gctccggtgg atcgaatact gatccaggct ccggtggctc cgcgggttcc ggtggctccg
     2281 cgggttccgg tggctccgcg ggtaccggtg gctccgcggg ctccggtggc tccgcgggtt
     2341 ccggtggctc cgccggctca cccactgacc ctggcaaagc cgaaatctgg gaggaaggac
     2401 tggctcccgg cgtcaatatg ggcatcgccg cgcaataccc cgacgacgtc ggtatctcct
     2461 cccatcccgc cgtctacgcc gcggaagact tcgaatccgg gtcagtgcaa ctgcccaccg
     2521 aagaagaccg cctggtcaac aaccttaccg tggtcgataa cgaagtatat accggccact
     2581 atgccgccgc cttttcctgg ccagaaggct tcaacggccc taccacacgc ttcgccctgc
     2641 ccgccgaccc aaacgacaat ccccacggcg cctacttcgt gcgcgtctgc tataacttcg
     2701 acaaaagctt tcatcccggt gatcaaagcg tcggcgtggg agtcaaaggc tttggcatct
     2761 atgccgaggg cgaaggcacc accgaaacga cctggtacaa tacctccgta caattcgtcg
     2821 gctggggagg cagttcgaaa ccccaggcca acgacggata cctctgggtc ggtcacctct
     2881 atagctacaa tccccatccc gacgaagccg tggctaccgt tggcgaactc gacaatgttt
     2941 ccgaataccg attctccgct tacgctactc ccttcgacta tatccgattc aatacctggc
     3001 gctgttacga agtgggacta tacctcaata ccccgggaga aaatgatggc gaagcgcgat
     3061 tctggatcga tggagtattg caaagtcgag tcacccacat gcgttttcgg gatagcatca
     3121 caccgcggcc cgataaggtg aatatcagcc tgcaccgcgt aaccgaaaat atcccccata
     3181 ccatgactcg ctggatggat aatatcgtca tcgcacgtcg ctatatcggc cccgtaaagc
     3241 tctcctcccc ctgagctatc tccgcatttt cccagctatt tccttcgccc gacaactgcc
     3301 agaaaggtct cccgaccgtc gctcggccca gcccatcgtg ggtccacatt cacggcccac
     3361 gctaatcgct cacggcccac ggtcacggtc acggcccacg gcaccatggg ggcgtacttt
     3421 gggaagaaag aacgcaagat agttggccaa gtttt
//
LOCUS       DAOSOR010000615         3454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_27391_length_3454_cov_3.326272, whole genome shotgun sequence.
ACCESSION   DAOSOR010000615 DAOSOR010000000
VERSION     DAOSOR010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27391_length_3454_cov_3.326272"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PK710_22515"
     CDS             complement(<1..606)
                     /locus_tag="PK710_22515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12571.1"
                     /translation="MIRKIAPLAFGLWLCACAGASPLMYPAHPLSDGNVRFAAGAAAN
                     FALGTPSHAIDEAAAEPVGVMADRPTEKTIRGALAVAAMAPGVSPLVSGRVGLGYDME
                     GGLTYTGRAAHLDARWAWVADSLALSFGAGASALLSRRGASPDMPIAGLDLDATTGWG
                     VDVPIVFGWHSDADMVWWWTGTRGGYERLRGDVGYRGPAPAL"
     gene            complement(613..936)
                     /locus_tag="PK710_22520"
     CDS             complement(613..936)
                     /locus_tag="PK710_22520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HOT12572.1"
                     /translation="MALFINDNCISCGACEAECPNEAIEYRGHQYAIVPERCTECVGF
                     RQQPACQDVCPSEACVRDPNHPESKPNLIRKVLALHPDDESVRRRIRCMQPEEQDDPP
                     SDHQG"
     gene            1049..1720
                     /locus_tag="PK710_22525"
     CDS             1049..1720
                     /locus_tag="PK710_22525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12573.1"
                     /translation="MLRSIRTVTKAWKSVFQRSCGLTKLSYGAPTMGRLSFWMYAISL
                     WVAGVALGCNPEIGDDCTTSTDCSINGDRLCDTTQPGGYCTIFNCEPDTCPEEAQCVA
                     FDTELDPTCQDQRWARFERTFCMLRCKGPGDCRSGYICSDMNAPNNPWGAHIADQHPD
                     GTKVCIAPWSGATPGAGLTEVCSPYDGGFPEADSVATSDASSDDSGAIDDAQDGSGDS
                     EGDDL"
     gene            1745..2782
                     /locus_tag="PK710_22530"
     CDS             1745..2782
                     /locus_tag="PK710_22530"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT12574.1"
                     /translation="MALFHRPLPRTLEAALRDLKEKSVLVRRSAVQDLGRHAREQATS
                     AIEEALLEALRDDDPLVRTEAAYCLGDMKSKRALPLLLVTVDDANSLVRQAAIDALGT
                     IGDPQATGRLLRALEDERPDVRFQAVIALGRISVDTGMDAVLRAVCDEDPHIRYIAVR
                     VAEELVTKELSPTDTASLPERLVEQAKGWLEDEDAHVRLAAAILLGKCGDDAGAEHLL
                     DAIEGRIFPMEPEDEAAAIELAGTLKLHQAIAGLERRAFGWKRWIKEQHVDTARVALV
                     RLGHQRARRAIVSDLRSWSRNQRTVAVTTAGQAGIREAIPLIEAMRGDHEQAVQEAVD
                     EALELLHARND"
     gene            2798..>3454
                     /locus_tag="PK710_22535"
     CDS             2798..>3454
                     /locus_tag="PK710_22535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12575.1"
                     /translation="MQGLSNWNAVRYDSQQPSAHVESYFLKAHDPKTPRALWLRASII
                     APCVRPAHAIAEAWAIVCEQDGSLTVVKESLPVADTRFSKEELLVSIGSFFHLDAKTC
                     HGAIQRTDHQIRWDLHHASTAKAWVHSPLPAAIEHAVSASHFASPFPDLRIDGSLSVD
                     GRTIDVSGWQGMQGHHWGRAHPPFMAWGHCNQWEQQVDTVVEAVSIRERLGPILGPLW
                     T"
BASE COUNT          679 a          908 c         1115 g          752 t
ORIGIN      
        1 aagagcgggg gcggggccac ggtatcccac atcgcctcga agccgctcat agccaccgcg
       61 cgtcccggtc caccaccaca ccatatccgc gtcgctatgc cagccgaata cgatggggac
      121 atctacgccc cagcccgtgg ttgcgtcgag atccagcccg gcaatgggca tatccggcga
      181 cgcgcctcgt cgggaaagca aggcgctagc tcccgctccg aacgataaag ccaaggaatc
      241 cgctacccaa gcccagcgag cgtccagatg agcggcgcgg cccgtatagg ttagtcctcc
      301 ctccatgtcg tagccgaggc ccacgcgtcc agacaccaag ggtgataccc cgggggccat
      361 ggcggctacg gcgagggcgc ctcggattgt tttttcggtg ggacggtcgg ccatgactcc
      421 cacgggctcg gccgcagcct catcgatagc gtgcgacggg gtgccaagcg cgaagttagc
      481 agccgccccc gcggcaaacc gtacgtttcc gtcggaaagc ggatgggcag ggtacatcaa
      541 cgggcttgcc cctgcacaag cgcataacca cagaccaaag gcgaggggtg ctatctttcg
      601 tatcacggca actcatcctt ggtggtcgga cggcggatcg tcctgctctt cgggttgcat
      661 gcatcttatc cttcgacgca cgctttcatc gtctggatgc agggcgagca ctttgcgaat
      721 gagattgggt ttggactctg gatggtttgg gtcgcggacg caagcctcgg atgggcaaac
      781 gtcctggcag gctggttgct gtcgaaatcc gacgcattcg gtgcatcgtt cggggacgat
      841 ggcatattga tggccgcgat actcgatcgc ctcgttggga cattccgcct cacaagcgcc
      901 gcagctaata caattgtcgt tgatgaacag cgccatgggt tggtctcgaa gcgaattcgg
      961 atatgtccat gatattcata ggaaccttct gcacactgtg tcaacgtagg ccgtggagtc
     1021 gataggtgtt gagcaagtgg gagtggggat gctgcggtcg atcaggacgg tgacgaaggc
     1081 gtggaagtcg gttttccaac ggtcttgcgg attgacgaaa ttgagctacg gtgcgccgac
     1141 catgggccga ttgtctttct ggatgtatgc gatttcgctg tgggtggcgg gtgtagcgtt
     1201 gggctgcaat cccgagattg gcgatgattg cacaacttcc acggactgtt cgatcaatgg
     1261 tgatcgtcta tgtgatacga cgcagcctgg ggggtactgc accattttca attgcgaacc
     1321 cgacacttgt cctgaggaag cgcaatgtgt ggcgttcgat acggagttgg acccgacgtg
     1381 ccaagaccag cggtgggctc gtttcgaacg gactttttgc atgttgcgat gcaaggggcc
     1441 tggtgattgt cggtctgggt atatttgcag cgatatgaat gcgccgaaca atccttgggg
     1501 ggcgcatatt gcggatcagc atcccgacgg caccaaggtt tgcattgctc cttggtcggg
     1561 agctacccct ggtgctggac ttacggaagt gtgcagtcct tacgatggcg ggtttccgga
     1621 agctgattcc gtggcaacgt cggatgcatc ttcggacgac agcggtgcca tcgacgatgc
     1681 gcaagatggt tccggcgatt ccgaagggga tgacctttga cgacgcggtg tcacggggga
     1741 gtccatggca ttatttcacc gcccgttacc acgaacgctc gaggcagccc ttcgcgatct
     1801 caaagaaaaa agcgtgttgg tgcgtcgttc cgcggtgcaa gacctcggtc gacatgcgcg
     1861 agagcaggcc acatcggcca ttgaggaagc tctgctcgaa gcgttgcgag acgatgatcc
     1921 gttggttcgt acggaagcgg cttattgtct tggggatatg aagtcgaagc gggcgttgcc
     1981 tttgctgttg gtgacggtgg atgatgcgaa ttcgttggtg cggcaagcgg cgatcgatgc
     2041 gttggggacg ataggagatc cgcaggcgac ggggcgtttg cttcgtgcgt tggaggacga
     2101 gcgtccggat gtgcgatttc aggcggtgat tgcgctcggg cgaatctcgg tggatacggg
     2161 aatggacgca gtattgcggg cggtatgcga tgaggacccg catattcgtt atattgcggt
     2221 gcgcgtggcc gaggagttgg tcaccaagga gctgtcgccc acggatacgg catcgttgcc
     2281 ggaacgattg gtggagcagg caaagggatg gttggaggac gaagacgcgc acgtgcgatt
     2341 ggctgcggcg attcttttgg gaaaatgcgg tgatgacgcg ggcgccgagc atttgctcga
     2401 tgccatagaa ggacggattt ttcccatgga acccgaggat gaagctgcag cgatcgagtt
     2461 ggcgggaacg ttgaaattgc atcaggccat cgcgggattg gagcgtcgcg cttttggctg
     2521 gaaacgttgg atcaaagagc agcacgtgga caccgcgcgc gtggctttgg tgcgtctggg
     2581 tcaccaacgg gcgcgacgag ccattgtgtc ggatcttcgt tcttggtcgc gaaaccagcg
     2641 tacggttgcg gtgacaacgg ccgggcaagc tggcatccgt gaagccattc ccctcatcga
     2701 ggcgatgcgt ggggatcatg agcaagcggt gcaagaagca gtcgacgagg cgctggaact
     2761 tttgcatgct cgtaacgact gaggagggtc gaaccccatg caaggtttgt cgaactggaa
     2821 tgccgttcgc tacgattcgc aacagccgag cgctcatgtc gaaagctact tcctcaaagc
     2881 ccacgacccc aaaacgcctc gtgccttgtg gctccgagct tcgattatcg ctccttgtgt
     2941 gcgtccagcc cacgccattg cagaagcatg ggccatcgta tgcgaacagg atggctcatt
     3001 gacggtcgtc aaagaatcgc ttcccgtggc ggatacgcgt ttctccaaag aagagttatt
     3061 ggtaagtatt ggctcttttt tccatctcga tgcgaagact tgtcacggag ccatccaacg
     3121 caccgatcac caaatccgct gggacctcca ccatgcatcg acagccaagg catgggtaca
     3181 ttctcctttg ccagcggcaa tcgagcatgc cgtatctgcc agccactttg cttctccctt
     3241 tcccgacctg cgtatcgatg gttcgctgtc tgtcgatggt cgaacgatcg atgtctctgg
     3301 ttggcaaggg atgcaggggc atcattgggg tcgtgctcat cccccgttca tggcctgggg
     3361 acattgcaat caatgggagc agcaggtcga tacggttgtg gaagcggtca gtatccgcga
     3421 gcgtttgggc ccgattctcg gcccgttatg gacg
//
LOCUS       DAOSOR010000616         3434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_27601_length_3434_cov_4.621782, whole genome shotgun sequence.
ACCESSION   DAOSOR010000616 DAOSOR010000000
VERSION     DAOSOR010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27601_length_3434_cov_4.621782"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1717
                     /locus_tag="PK710_22540"
     CDS             <1..1717
                     /locus_tag="PK710_22540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOT12576.1"
                     /translation="PVVLNQLYRKTDLQSSYGVINLAIVKGRPEVLDLKSALNHFVEH
                     RREVVTRRTRFELHKAEAEREIVEGLGMAITQVDLVIKTIRESPDPDTARLRLMGVPL
                     QGLEQFVRRAGRPEEEIQAAAQRKEYLLSERQARAILEMRLARLTGLEQEKLAVQYGK
                     LCEDIERFRSILADDSVLMNVIVTELEDIRERFANPRRTVIVSTEVEINVEDLIQEED
                     MVVTVSHLGYIKRTSAAAYRAQRRGGKGKLGMQARDEDFVQRLFVASTHATVFFFSDK
                     GKVYRKRVFEIPEAASNARGRAVVNFVGMEPGEKIAAITLVSDFEEGQFVVTLTKKGQ
                     IKKTSVVEFQNVREKGIIGVRIEDDDELLSAAVTDGQREFIIATRGGQSIRFPEEQVR
                     ATGRATMGVKAVDLDDGDEVVGMVVTMETCGHVLAVCENGYGKRTVLQEFRVQNRGGK
                     GVRLIETSERNGPVVGIGLVCDADELMMVTDRGQMIRTKVAEIRETGRYAQGVKIMTL
                     GESERVVALERIAEREEEYEGDGNGTDGEEGNRNGNGSSRTYRSTRPPSRYSERPPDS
                     GSDDG"
     gene            1724..2416
                     /gene="nth"
                     /locus_tag="PK710_22545"
     CDS             1724..2416
                     /gene="nth"
                     /locus_tag="PK710_22545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOT12577.1"
                     /translation="MAPKSKAIRYALTTFERLRAHHADADCELVPRDPFQLLVAVVLS
                     AQTTDAAVNRVLPDLFATMPDAASFATAQPEQVAEKIKTIGMYRQKAKHLVGLAKQIM
                     EHHQGQVPRQFDALVQLPGVGRKTANVVMGVAFGIPSGVVVDTHVQRLSQRLGWTKQQ
                     QPESIEKELSAVFPKEVWIILSHVLIFHGRRVCTARAPACERCVVSDVCPSAGKAENV
                     GRKPSRMRSLRE"
     gene            complement(2526..>3434)
                     /locus_tag="PK710_22550"
     CDS             complement(2526..>3434)
                     /locus_tag="PK710_22550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HOT12578.1"
                     /translation="ILGGDSGITLRDMLAAAALLKSKRIPSRLEFLLAPPSRQILEIL
                     TTTGALLDLIATGARLIEPDHRILTGRLYSPAHGENSLRTFDPEPTARTEPSFLVASA
                     ETLAFAIANGQLDDPRSFKRPVRVTVPRVFPTDDVLIARPRKSTLATIGKKLVVATVP
                     AVGFDSELDLTVHVGVPEADSFEHNKIALVARNDHEIRRVESLATSWLGAVRAVVAEH
                     IPTEIALWLGGLGIAAFRLPCVQDLHDGNVLHLPAPARWPADGQVEANIGKNTIVLTW
                     LGRSGEKEILRSGLYRPPHPHAKANA"
BASE COUNT          778 a          810 c         1144 g          702 t
ORIGIN      
        1 tcctgtggtg ctcaatcagt tgtatcgaaa aacggatctt caatcgtcgt atggtgtcat
       61 caatctcgcc atcgtcaaag ggcgtcccga agtcctcgat ctcaagtctg cgctgaatca
      121 tttcgtggag catcgtcgtg aggtggtgac acggcgaacc cgtttcgaat tgcacaaagc
      181 ggaagccgag cgggaaatcg tcgaaggtct cggcatggcg attacgcagg tcgatctggt
      241 catcaaaacc atccgggaat caccggatcc ggataccgcg cggctgcggc tcatgggggt
      301 gccgttgcaa gggctcgagc aattcgttcg tcgcgctgga agacccgaag aagaaataca
      361 ggcggctgca caacgcaagg agtacctgct ttccgagcgt caagcgcgcg ccatcctcga
      421 gatgcggctc gcgcgattga ccggcctcga gcaggaaaag ctggccgtgc aatacggcaa
      481 gctttgtgaa gatatcgagc gatttcggtc tattttggcc gatgattccg tgctcatgaa
      541 tgtcatcgtc acggaactgg aagacattcg cgaacggttt gccaatccac gccgaacggt
      601 catcgtttcc acggaagtcg aaatcaacgt ggaagacctc attcaagaag aggatatggt
      661 cgtgacggta tcccatctcg gatacatcaa acgtacgagt gcggccgctt accgggcgca
      721 acgacgaggt ggaaaaggca agcttggcat gcaagcgcgg gacgaggatt tcgtgcagcg
      781 gctatttgtc gccagtacgc atgccacggt gtttttcttc tcggacaagg gaaaagtcta
      841 tcgaaagcgt gtgtttgaga ttcccgaggc ggccagcaat gcccgaggtc gtgcggtcgt
      901 gaacttcgtg ggcatggagc ccggcgaaaa aattgccgct attacgctcg tcagcgactt
      961 tgaagaagga caattcgtcg tcaccttgac gaaaaaaggg cagatcaaaa aaacgtcggt
     1021 ggtggagttc caaaacgttc gggaaaaggg gattattggc gttcgtatcg aggacgatga
     1081 cgaattgctt tcggcagcgg tgaccgacgg ccagcgggag ttcatcattg ccacgcgagg
     1141 aggccagtcc attcgttttc cagaagagca ggttcgcgcc accggacgag ctacgatggg
     1201 ggtcaaggcg gtggacttgg acgacgggga cgaagtggtg gggatggtgg tgaccatgga
     1261 aacgtgcggt cacgtgttgg cggtgtgtga gaatggctat ggtaaacgga ccgtattgca
     1321 ggagttccgc gttcagaatc gaggtggcaa gggcgtgcgg ttgatcgaaa cgagcgagcg
     1381 caatggcccg gtggtgggta ttgggttggt atgcgatgcg gatgaactga tgatggtcac
     1441 ggatcgcggg cagatgattc gtaccaaggt agcggaaatc cgcgagacgg gtcgatacgc
     1501 gcaaggcgtg aaaatcatga ccttgggcga aagcgagcgg gtcgtggccc tcgaacgaat
     1561 tgccgaacga gaagaagagt atgaggggga cggcaacggt acggatggcg aggagggaaa
     1621 tcgaaatggc aacggctctt cgcggacgta ccgttccaca cgcccgcctt cgcggtattc
     1681 cgaacgtccg ccggacagcg gttccgatga cggctgagtg ttcatggctc caaaatccaa
     1741 agcgatccgt tatgccctaa cgacgttcga gcgtcttcgg gcgcatcatg cggatgcgga
     1801 ttgtgaactc gttccgcgcg atccatttca gttgctcgtg gcggtggtgc tcagcgcgca
     1861 aaccaccgat gccgccgtga atcgggtgct tccggatctg tttgccacga tgcccgatgc
     1921 ggcgagtttt gcgacggcgc agcccgagca ggtggccgag aaaatcaaaa cgatcggcat
     1981 gtatcgtcag aaggccaagc acctggtggg acttgcaaaa caaatcatgg aacatcacca
     2041 ggggcaggta ccgcgacagt tcgacgcgtt ggtgcaattg cctggtgtcg gaagaaagac
     2101 cgccaatgtg gtgatgggag tggcgtttgg tatcccttcg ggggtggtgg tcgataccca
     2161 tgtgcagcgt ctttcccagc ggttgggctg gacgaagcag cagcagcccg aatcgatcga
     2221 gaaggagtta tcggccgttt ttccgaaaga ggtttggata attctatcgc atgtattgat
     2281 ttttcatggt cgaagggtgt gtacggcgcg cgctcctgct tgtgaacggt gtgtggtttc
     2341 ggacgtttgt ccgagtgcgg gaaaagcgga gaacgtgggg agaaagcctt cgaggatgag
     2401 atcgttgcgg gaatagcctc gatcgtgaac gttgatggtt aatgaaatgg aaagagattg
     2461 catactatcc gcatatggat agcctacgat acggggctga tcagaaacga acggtggtgc
     2521 aacgattatg cgttcgcttt ggcatggggg tgtggtggac gatacaaacc cgaccgcaag
     2581 atttcttttt ctccggatcg accaagccag gtgaggacaa tcgtgttttt gccgatgttg
     2641 gcttcgactt gtccatccgc aggccagcgg gcgggtgcgg gaagatgcaa tacgttgcca
     2701 tcgtgaagat cttgaacgca agggagacgg aacgcggcga ttccgaggcc gccgagccat
     2761 agcgcgattt cggtgggaat gtgttcagcg acgacagcgc ggacggcacc gagccatgag
     2821 gtagccaatg actcgacacg tcgaatctcg tggtcatttc ttgccacgag ggcgatcttg
     2881 ttgtgttcga aggaatcggc ttccggcacg ccaacatgga ccgtcaggtc aagctcggag
     2941 tcgaagccga ccgcgggtac ggtagcaacc accagttttt tcccgatggt cgctaacgtg
     3001 ctttttcgag ggcgggcaat gagaacgtcg tcggtgggga aaacacgggg cacggtgaca
     3061 cgaaccgggc gtttgaagct tcggggatcg tcgagctggc cgttagcgat cgcgaaagcg
     3121 agagtttcgg cggaagcgac gaggaaagag ggctcggtgc gagcggtggg ttcgggatcg
     3181 aacgtgcgca gagagttttc gccatgggca ggggagtaga gtcgtccggt caggatacga
     3241 tggtcaggtt cgatcagacg agcgcccgtg gcgatgaggt cgagaagggc gccggttgtg
     3301 gtgagaattt cgaggatttg gcgcgaaggg ggagctagca aaaactcgag ccgtgaagga
     3361 atccgtttgg acttgagcaa ggccgcggcc gcgagcatgt cccgcaacgt gatgccgcta
     3421 tcaccaccaa gaat
//
LOCUS       DAOSOR010000617         3434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_27602_length_3434_cov_4.289435, whole genome shotgun sequence.
ACCESSION   DAOSOR010000617 DAOSOR010000000
VERSION     DAOSOR010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27602_length_3434_cov_4.289435"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="PK710_22555"
     CDS             <1..954
                     /locus_tag="PK710_22555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12579.1"
                     /translation="DGHFGALLWDDKTDSIFVVRYDFSGNELWSTKLTNSDNKPTDFG
                     IGDSRLEYGDGKYGAYYHVHSDSGHEGDTLKWVTTAGVETTGWKWGCSHSMSNLLRFN
                     ASLSKFMPACVTDCFPGTSGDFSTNSIGGIYLNHTSGPVLNVDAGCNGSVAGELGGAA
                     EGTAGWKLVFNAHQAPAKKGQQSYSPATMNQDIGFASIKGNFSSDPVVWLTDTSSINE
                     ADATIARWQPKGETKEQYVVGWAEPGSSYVYKLARVDASGAFLEGPTDITSLARWGRR
                     DDPMRVHIGGDVVWAWFESPGSTQLHLARLVSGNTPTCASF"
     gene            complement(982..2820)
                     /locus_tag="PK710_22560"
     CDS             complement(982..2820)
                     /locus_tag="PK710_22560"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HOT12580.1"
                     /translation="MRKPFRERLEEGTVLFDGGMGTMLYTKGIYINTCFDELNITAPH
                     LVRQVHDEYLRAGADAIETNTFGASSYKLEPFGLAQKLHEINLRGAQIAREAAGDDVY
                     VAGAIGPLGVPIEPIGRVGFDEARADFAKRVRALREGGVDLIILETFLYLSELEQAIL
                     AVRDECDLPVIAQVVIRNGETLAVGTPISDAVARINGFYPDMVGFNCAVGPKEMLDAL
                     ETAVALTSRPISLMPNAGEPRRVEGRTIYLGSPEYFAEFSKRFVQTGARIIGGCCGTT
                     PEHIKAMRRAIAALEPAPRHQIQVRELQAQAPPEITPYSPQDKSRLSCRLADHKYVTL
                     CELVSPKGVDAERVLKQARQLAHHGVDAINIPDGPRASSRMSALALAILIQRNVNIEV
                     LLHYTCRDRNVIGMQSDLLGAWALGIRNILAVTGDPPKLGNYPDATAVYDVDAIGLTN
                     LLWRLNHGLDIARNPIGEPTGFLVGVGANPGAINLEEELRRLEYKIEAGAEFIITQPV
                     FDLRIFETFLRRIEPIRVPVLSGIWPLVSLRNAEFLNHEVPGASVPDDVMEKMRAAKS
                     KEEALAVGTAVAQQMVRHVRPHTQGIQIAVPFGRVDMVLEVLDGVL"
     gene            3165..>3434
                     /locus_tag="PK710_22565"
     CDS             3165..>3434
                     /locus_tag="PK710_22565"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT12581.1"
                     /translation="MALEREELHLLLDTLRSFCDRNLPLARRLELDANHTYPADVIEG
                     LFGEVGLHLLFLPTSCGGTDGGAFDIYRVSELMAQYDLGVATAVLA"
BASE COUNT          689 a          818 c         1132 g          795 t
ORIGIN      
        1 gacgggcatt ttggagcatt gctctgggat gacaagaccg acagtatttt cgtggttcgt
       61 tatgattttt ccggcaacga gctgtggtcc acgaagctga cgaattcgga taacaagccg
      121 acggatttcg gcattgggga cagtcgtctc gagtatggcg atggtaagta cggggcttat
      181 taccatgttc attcggattc gggtcatgag ggggacacgt tgaagtgggt gacgacggct
      241 ggtgtggaga cgacgggatg gaagtggggg tgttcgcata gcatgagcaa tttattgcga
      301 ttcaacgcgt cgctcagcaa gttcatgccc gcgtgtgtga cggattgttt tcctgggacg
      361 agtggtgatt tcagcaccaa ttccattggg ggcatttatt tgaatcacac gagcggtccg
      421 gtgttgaacg tggatgctgg atgcaacggt agtgttgcgg gggaattggg gggagctgcc
      481 gagggcacgg ctgggtggaa gctggtgttc aacgcgcacc aggcgcctgc caagaagggt
      541 cagcagagtt attcgcccgc gacgatgaat caggacattg gatttgcctc catcaaaggc
      601 aatttctctt ccgatccggt ggtttggttg acggatacgt ccagcatcaa cgaagcggat
      661 gccacgatag ctcgttggca accgaaagga gaaacgaagg agcaatatgt ggttggctgg
      721 gcggagcccg gttcctccta cgtctacaaa ttggcaaggg tcgatgcttc cggagcattt
      781 ttggaagggc cgacggatat cacctcgttg gcgcggtggg ggcgccgcga cgatccgatg
      841 cgggttcaca taggtggtga tgtcgtgtgg gcctggttcg aatccccggg aagtacgcaa
      901 ttgcatttgg cccggctggt ttcgggcaat acccccacct gcgcttcgtt ttgaaccgtc
      961 tttgtcagta agaaacgatg actacaaaac gccgtcgagg acttcgagca ccatatcgac
     1021 acgtccgaag gggacggcga tttggatacc ttgggtatgc gggcgaacgt ggcgaaccat
     1081 ttgttgagcg acggctgtcc ctacggcgag agcttcttct tttgatttgg ccgcgcgcat
     1141 tttttccatg acgtcatcgg gcacggaggc gcctgggact tcatgattga ggaattcggc
     1201 atttcgcaac gaaacgaggg gccagatccc ggaaagcacg ggtacgcgga tgggttcgat
     1261 acggcgcaaa aacgtttcga agatgcgcag gtcgaacacg ggttgggtga tgatgaattc
     1321 ggcacccgct tcgattttat attcgagtcg tcgcagttct tcctcgaggt tgatggcacc
     1381 agggttggcg ccgacgccga cgagaaagcc ggtgggttcg ccgatggggt tgcgggcgat
     1441 atccaggccg tgattgagtc gccacagcag gttggtcaag ccgatggcgt cgacatcgta
     1501 gacagcggtg gcatcggggt aattgccgag tttggggggg tcgccggtga cggcgaggat
     1561 attgcgaata ccgagggccc aagctcccaa caggtcgctt tgcataccga tgacgtttcg
     1621 gtcgcgacag gtgtagtgaa ggagcacttc gatattgaca ttgcgttgaa tgaggatggc
     1681 gagggccagg gcggacatgc gcgagcttgc tcggggacca tcggggatat tgatcgcatc
     1741 aacgccatgg tgggcgagtt gtcgtgcttg tttgagcacg cgttcggcgt cgacaccttt
     1801 gggtgatacg agttcgcaga gggtgacgta cttgtgatcg gcgaggcggc atgacaagcg
     1861 tgatttgtct tggggggaat agggagtgat ttcgggaggt gcctgggctt ggagttcacg
     1921 aacttggatc tggtgtcgag gggctggttc gagcgcggcg atggcgcgtc gcatggcttt
     1981 gatgtgttca ggcgtcgttc cacagcatcc gccgatgatt cgtgcgccgg tttgcacgaa
     2041 gcgtttcgaa aactcggcaa agtattcggg tgatccaagg tagatggttc ttccttcgac
     2101 gcgtcgcggt tctcccgcgt tgggcatcaa agaaatgggg cgagaggtaa gggccacggc
     2161 cgtttcgagg gcatcgagca tttccttggg accgaccgcg caattgaaac caaccatatc
     2221 gggatagaac ccattgatcc gcgccacggc gtcggaaatg ggagtgccca cggcgagggt
     2281 ttcgccattg cgaatcacga cttgcgcgat cacgggcaga tcgcattcat cgcgaacggc
     2341 aaggatggcc tgttcgagtt cggataggta caaaaaagtt tcgaggatga tgaggtccac
     2401 gcctccttct cgaagagcgc gaacccgttt tgcgaaatcg gcgcgtgctt cgtcgaagcc
     2461 gacacgtcca atcggttcga tgggcacgcc gagaggaccg atggctccgg cgacgtagac
     2521 gtcgtcacca gccgcttctc ttgcgatttg ggctccccgc aaattgatct cgtgcaattt
     2581 ctgagcgagt ccaaagggct cgagtttata gctgctggca ccaaacgtat tggtttcgat
     2641 ggcgtcggct cccgcgcgta agtattcatc gtggacttgg cgaaccaagt gcggtgccgt
     2701 gatgttgagt tcgtcaaagc aggtgttgat atagatgccc ttcgtgtaga gcatcgttcc
     2761 cattccaccg tcgaacaata cggtgccttc ttcgaggcgc tcccgaaatg gttttcgcat
     2821 ggtctatcct ctgtcgttga tgggagtgca tagtgtagga ggtgggccgc ggtcatggga
     2881 aagcgatgcg aggtgggggg gcgaggcggt ctttcgaggc tggataattc ttcgaaaccc
     2941 cgatcggaga gacgaaatcg tacctgttcg tttgaatcgt gcaacgcacg aagggcgggg
     3001 acggtcggtt tttgcgacat tcagaggatt acaacgcgaa gcgtcaagac aactctcgca
     3061 tcggacgtgt gcgggaatgt ccgacccgtg ttatgcccct gcccccaacg cggtgactgc
     3121 aagtggttgc cgcatggctt ctgcgattgg aacgaggaat ctcgatggct ctcgagcgtg
     3181 aagaactaca cctgcttttg gacaccctcc ggtccttttg tgatcgcaac ctaccgttgg
     3241 cgcgtcgact cgaactcgat gcgaatcata cgtatcccgc cgacgtcatc gaaggattgt
     3301 ttggcgaggt gggactgcat ttgctgttct tgccgacatc ctgtggaggc acggatggtg
     3361 gagcgttcga catctatcgc gtcagtgagt tgatggcgca gtacgacctg ggtgtggcca
     3421 ctgccgttct ggcc
//
LOCUS       DAOSOR010000618         3418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_27764_length_3418_cov_3.690158, whole genome shotgun sequence.
ACCESSION   DAOSOR010000618 DAOSOR010000000
VERSION     DAOSOR010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27764_length_3418_cov_3.690158"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="PK710_22570"
     CDS             complement(<1..1233)
                     /locus_tag="PK710_22570"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOT12582.1"
                     /translation="MTKKLLLSSVFGPFGVDDAFGHKENLMELFHNQVTKAQGIASLR
                     FHHRSFGLYFLAENVRAPVTVLDFPSRNDFERELKRQRYDAVGISFIVPNFLKAKEMA
                     RLVRQHQPEAQILLGGHGAAIEGIENLIDCDHVVRGEGIRWLRQYLGEDPHAPIHHPT
                     LISAEHKRLFGIPTPGACGLLVPGVGCVNGCRFCATSHFFGRTYTPFFASGHALFEQL
                     CRMSNELGTQEFFVMDENFLKDKTRAQELLSCMEENHRPFSFAVFSSAETIEAFGVEN
                     LARLGVFFLWIGAESKHETYDKNKGRNLQALIRNLRDHGIAVLVSGILFLEHHRPDTI
                     SQDIDYLIDLQGVFTQFMMFTPLPGTQLYKQYKRQNRIDFDLPFEQWHGQHLLNFHHP
                     HFTRAQATQWLQEAFQQEF"
     gene            1393..2331
                     /locus_tag="PK710_22575"
     CDS             1393..2331
                     /locus_tag="PK710_22575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT12583.1"
                     /translation="MSRTAMSRWWKIPAGPVFWMVTVQVAWTITLVAWIIYFVERREE
                     LEGWSTLVAGILLLCLLLAGVTVIVIHFGRQVAHNRAVKDFVSTVSHDLRSPLATVKL
                     HLQTLEYRTLSPEQQTLCLATALEELNRLESGIEDILTASRVERGNLRLDKDPVELAD
                     FLTSYCDDKGRMLSLHDAVLSWTPPQTQRFKVRADPKLLRQMLDNLIDNALVHGPRGV
                     KIDLGLSERGGAAVIHVSDNGPGIVRSQRKKIFRMFYRAPGHRKHRKGTGLGLFIVAS
                     IAKAHSGRAWVDSDGAGKGSTFHVAIPFAKEERNPS"
     gene            2328..3020
                     /locus_tag="PK710_22580"
     CDS             2328..3020
                     /locus_tag="PK710_22580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT12584.1"
                     /translation="MKQRLLLVEDEENLAKGLELNFSLEGFSVQWVANGVDARTSWKK
                     EMPDLVVLDLGLPDTDGLELLQEIKRVDLRVPVLVLTARASDEERITGLSLGADDYVT
                     KPFNLTELVLRVRGLAQRSQWYRTSLPKEICLGAAVLTPSDATLRRDGETYWLTELEL
                     RLLLHLWRQRGSYVSREELLVQVWGYSAETATRTVDIFVSRLRRMLGDAANPVLLLTK
                     RGKGYMLVREGE"
BASE COUNT          704 a          803 c         1127 g          784 t
ORIGIN      
        1 gaattcttgt tgaaaagcct cttgcaacca ttgggtggct tgcgcgcggg tgaagtgagg
       61 gtggtggaag ttcagcaagt gttggccgtg ccattgttcg aaaggaaggt cgaaatcgat
      121 gcggttttgc ctcttgtact gcttgtagag ttgggttccc ggcaaggggg tgaacatcat
      181 gaattgggtg aaaactcctt gaagatcgat gagatagtcg atgtcctggg agatggtgtc
      241 ggggcgatgg tgttcgagaa aaaggatgcc ggaaacgagt acggcaatgc cgtgatcgcg
      301 caagttccgg atgagggctt gcaggttgcg gcctttgttt ttgtcgtacg tttcgtgctt
      361 ggattcggca ccaatccaga ggaaaaacac gcctagccgc gcgaggtttt ccactccgaa
      421 cgcctcgatg gtttcggcgg aggagaatac ggcgaagctg aacgggcgat ggttttcttc
      481 catgcaggaa agcagctctt gcgctctcgt tttgtctttg agaaagttct cgtccatgac
      541 gaagaactcc tgggtgccaa gttcgttgga catgcggcaa agctgttcga aaagggcgtg
      601 tccgctggcg aaaaacgggg tgtaggttcg accgaaaaag tggctggtcg cgcagaagcg
      661 gcagccgttg acacatccga ctcccggaac gagtagcccg cacgctcctg gggttggaat
      721 cccgaatagg cgtttgtgtt cggcggagat caaggttgga tgatggatgg gagcgtgggg
      781 gtcttcgccc aggtattgtc ggagccagcg gatcccttcg ccgcgcacga catggtcaca
      841 gtcgatgagg ttttcgatgc cttcgatggc ggcgccgtgg cctccgagga gaatctgtgc
      901 ctcgggctgg tgttggcgca ccaatcgagc catttctttg gctttgagga agttggggac
      961 gatgaagctg atgccgacgg cgtcgtagcg ttggcgtttc agctctcttt cgaaatcgtt
     1021 gcgggaagga aagtcgagca cggtaacggg agcgcggacg ttttccgcca ggaagtacaa
     1081 gccgaaggag cgatgatgga agcgaagcga cgcgatcccc tgggctttgg tcacctgatt
     1141 gtgaaataat tccatcaggt tttccttgtg cccgaaggca tcatccacgc cgaaggggcc
     1201 gaagacgctg ctgagaagaa gtttttttgt cattgtcgga caagcatgcg gcttgtctgt
     1261 ccgggcgagc gtaaaacatg ggtaaaatgg gatgtggcag ggatcctcgc ggctgttccg
     1321 attgtgctat ccatggggcc gagccatgaa tggtcgtttt ctacccgttg accagtccgg
     1381 agccgcactg ccatgagccg cactgccatg agccgttggt ggaaaatccc cgcggggcca
     1441 gttttctgga tggtgacggt gcaggtggcg tggaccatca ccctggtggc gtggatcatt
     1501 tacttcgtgg agcgaagaga ggagttggaa ggctggagca cgttggtggc tggcattctg
     1561 ctgctctgtt tgcttttggc gggcgtgacg gtcatcgtga ttcattttgg caggcaagtc
     1621 gctcataacc gtgcggtcaa ggacttcgta tccacggtca gccacgacct tcgctcgccc
     1681 cttgccacgg tcaagttgca cctacaaacc ctggagtatc ggacgctttc gccagagcaa
     1741 cagaccttgt gccttgccac ggccctggag gaactcaatc ggttggaatc gggaatcgag
     1801 gatattctca cggcttcccg cgtcgagcgc ggcaacctgc gtctcgacaa ggaccctgtc
     1861 gaactggctg attttctaac atcgtattgc gatgacaagg gaaggatgct ttcgctgcac
     1921 gacgccgtct tatcgtggac gccgccgcaa acgcaacgtt tcaaggtccg ggcggatccc
     1981 aaattgctgc ggcagatgct cgataacctt atcgacaacg cgcttgtgca tggtccccgg
     2041 ggcgtgaaaa tcgatcttgg gctgtccgaa cggggcggcg cggcggtcat tcatgtgagc
     2101 gacaacggtc caggtatcgt ccgatcccag cgaaaaaaga tatttcgcat gttttatcgc
     2161 gccccgggac atcgaaagca ccgaaaaggc accggtcttg gcctgttcat cgtggccagc
     2221 atcgccaaag cccattcggg gcgcgcctgg gtcgatagcg atggcgccgg aaaaggaagc
     2281 acctttcacg tggccattcc ctttgccaag gaagaaagaa acccatcgtg aaacaaagat
     2341 tattgctcgt cgaagacgaa gaaaaccttg cgaaagggct ggaactgaac ttttcgctcg
     2401 aaggattctc cgtgcaatgg gtggccaacg gtgtggatgc gaggacgagt tggaaaaaag
     2461 aaatgcccga cctggtggtg ctcgatcttg ggctgccgga taccgacggt ttggaattgt
     2521 tgcaggaaat caagcgtgtc gaccttcggg ttccggtgtt ggtgctcacc gcgcgagctt
     2581 cggacgagga acgtatcact ggtttgagtt tgggagccga cgattatgtg accaagcctt
     2641 tcaacttgac cgaactcgtg cttcgtgtgc gtggcctggc ccagcgcagc cagtggtatc
     2701 gaacgtcgtt gcccaaggag atatgcctgg gcgcggcggt attgacgcct tcggatgcca
     2761 cgcttcgacg ggacggggag acgtattggc tgacggaatt ggaactgcgg ttgcttttgc
     2821 atttgtggcg ccagcggggg agttatgtgt ctcgggagga gctattggta caggtgtggg
     2881 ggtattcggc ggagacggcg acgcggacgg tggatatttt cgtgtcgagg cttcgccgga
     2941 tgttggggga tgcagctaac cctgtgttat tactaacaaa acgggggaaa gggtatatgc
     3001 tcgtgcgcga gggggaatga gcgctggtgg aggcagggtg gaggttgtac gaatcgccga
     3061 acctgttttc ccacgacttg tgcagtatca gagtttgccg tgcgttgggg agggggttcg
     3121 gattttttac cggctgattc atcgatgcta agatctaaga tcgtggtgga agcttggatc
     3181 cgcccatgct acgagagaat ctatcttggg cttctgttgc ctgattgcag aaaacaagga
     3241 gacgtgggaa tgagaacatc gctttcttta tcggtagtcg cgctggcctt tcttctgaca
     3301 ggaggattga gtgcatgcag cgacgacgac gacaacaaca acacgggtgg cactgcgggt
     3361 agtgagaccg gtggcactgg tggtggggag accggtggca ctggtggtgg ggagaccg
//
LOCUS       DAOSOR010000619         3415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_27801_length_3415_cov_3.643750, whole genome shotgun sequence.
ACCESSION   DAOSOR010000619 DAOSOR010000000
VERSION     DAOSOR010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27801_length_3415_cov_3.643750"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="PK710_22585"
     CDS             complement(<1..973)
                     /locus_tag="PK710_22585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12585.1"
                     /translation="MKRLLIYGIPVGALFFPLMQACSSDDEDPSSPSKGGSGGAVTDA
                     QSDHQNDTSTGGGSGGGTSGTGGSIAGSGGTDVPDAPEDSEPDVPEDIVVGDVICPAG
                     SFQCKNNTLLKCNETGTQWIDYWPCATSALCDDQQGRCHPPVCQPSEHRCVGATLEIC
                     LSNQTGWSTKEVCESAAHCRAEYQTCAATPCSPNTMQCSGSVLQVCNGTGWDDVQDCK
                     SSTLCDSVAKTCTPSACAPGQVSCDGAKLRVCNFTLTDWHTLETCVSEALCDEAGSQC
                     DICLAKSYRCDGPKLWLCSEDGQQESLSQTCSSPQRCNALSGRCDPAC"
     gene            complement(1195..1737)
                     /locus_tag="PK710_22590"
     CDS             complement(1195..1737)
                     /locus_tag="PK710_22590"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HOT12586.1"
                     /translation="MTFRIPEGLHWWAIRTKANFEKTVFAQLTNRQFEAFLPTYKTYS
                     RRKDRRAIVSLPLFPGYVFLHSNLTDRERRVGVMQTRGMVQILGGPEGPLPVRDHEIT
                     SLQVMCNSDRMLEPLGRIERGKTVRIVSGGLAGVIGIVAEIKGKGRRIICNVNLLGRA
                     VAAELLPTDIEVVGPFDRFE"
     gene            1931..2779
                     /locus_tag="PK710_22595"
     CDS             1931..2779
                     /locus_tag="PK710_22595"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOT12587.1"
                     /translation="MLWPWTREQGQWTGNGLAFMVARMRRVDIETIHVSLWGDRLAQR
                     IGLGQVDAEFVIRVLEPFATERRREKLQRVFADRIGSVTVVMDSICDPHNGAALLRTC
                     EAFGVARIHLLEQGASFLAHAGVARGTHKWVEVRTHADAESCVKALRDDGMVLVGAHP
                     QGKLQAEDLQSIPRLALVLGNEHQGIRPVLTQACEHAVRVPMRGFAESLNVSVSGGIL
                     LAYATAHRPGDLPETERRRLYARGLAVTVPNAGALLERADHERFAGEQDNARQGPFTA
                     KNSLSD"
     gene            2875..>3415
                     /locus_tag="PK710_22600"
     CDS             2875..>3415
                     /locus_tag="PK710_22600"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HOT12588.1"
                     /translation="MKTPPLWYVCSQGAVFGPMNTVTLLQGVAEGHVPTDSLVRAHAW
                     SQWRNILQIREVSAFQKASNPSFEPRWELPYPEDEGQALARSFLRCAISSTEALLLAM
                     HAAVELTRAEVAMVHRYRGPERAFVTSFTHGDGLEPSVGHALPGYDAACVAAMDGRGV
                     WGSQGVGSAQRALVARLSRG"
BASE COUNT          714 a          909 c         1076 g          716 t
ORIGIN      
        1 cgcaagccgg atcgcatcgt ccgctgagcg cgttgcaacg ctggggcgat gaacacgttt
       61 gggaaaggga ttcttgctga ccgtcctcgg aacatagcca cagcttgggg ccgtcacatc
      121 gataggattt ggcaaggcaa atgtcgcatt gggagcctgc ttcgtcgcaa agcgcttcgc
      181 tcacgcaagt ctcgagcgta tgccaatcgg tgagcgtgaa gttacaaacg cgaagcttcg
      241 ccccatcgca ggatacttgt cctggagcac aagccgaagg cgtgcatgtt ttcgcgaccg
      301 aatcgcaaag cgtggaagac ttgcagtctt gtacgtcgtc ccaaccggtc ccgttacaaa
      361 cctgaagcac gcttccactg cactgcatgg tgttgggaga acaaggagtg gcggcgcagg
      421 tttggtattc cgcacgacaa tgggctgccg attcacacac ctctttggtg gaccagccgg
      481 tctgattgga caaacaaatc tcgagggtgg caccgacaca acgatgttcc gaaggttggc
      541 agacaggggg atggcatcgt ccctgctgat cgtcgcacaa cgcggaagtg gcacagggcc
      601 agtagtcaat ccattgtgtg cccgtttcat tgcatttgag cagggtgttg tttttgcatt
      661 gaaacgaacc cgcggggcaa atcacatcac cgacgacgat gtcctcggga acgtcaggct
      721 cactatcctc tggtgcatcg gggacgtccg tacctcccga accagcgatg gaaccgcctg
      781 ttcccgacgt tccgccgccg ctgcctccgc cggtgctcgt atcgttttga tggtcggatt
      841 gcgcgtcggt aactgctccc ccggagcctc ctttggaggg ggaggaaggg tcctcatcgt
      901 cggaactaca tgcttgcata aggggaaaaa acaaggctcc aacagggatt ccgtagatca
      961 acaagcgttt catgttcctt cactccacga cgattcgatg caacccagat gtggcgagag
     1021 agactcgcgc gacccggccc gcttcggttt ccgtcagtat gcgcctgtca gaagacaaag
     1081 caatgcgtgg cacgcgtggc tcggctttcg gcaagaatac cgaatgaaat gtgccaaagg
     1141 gaggttgacg gatgctctga gcgctgattg cgacgacaaa tcagtggcca tttcttattc
     1201 gaagcgatcg aaaggaccga cgacttcgat gtcggtaggg agcaattccg cggcgaccgc
     1261 gcgacccagc aggttgacgt tgcatatgat tcgacggccc ttgcctttga tttcggcaac
     1321 gattccgatc acgcccgcca atccgccaga cacgatacgt acggtctttc ctctttcgat
     1381 tcgcccgaga ggttccaaca tacgatcgga attacacatc acctgaaggc tggtgatctc
     1441 atggtcccga acgggcaagg ggccttccgg accacctaaa atctgaacca ttccgcgggt
     1501 ttgcatgaca ccgacgcgtc gttcccgatc cgtcaggttc gaatgcaaga aaacataccc
     1561 cggaaacaga ggcaaagaga caatcgcgcg acggtctttt cgtcgcgaat aggttttgta
     1621 ggtgggaagg aaggcttcga actgacgatt cgtgagttga gcgaagacgg ttttttcgaa
     1681 gttggccttg gtgcgaatcg cccaccagtg caggccctcg ggaatccgaa acgtcatggg
     1741 gacaacactt ctccgagctg tcaggcggga atatagacga tttgggcctg gtaagcgaat
     1801 gcgaaggcat ggtgcgtgaa gcgtgtgccg tgaccgtgta gcgtatgccc gcgaccgagc
     1861 ggactcccac caaaacggat gccgttcaca ggatggaata cactttcctg cccatggatg
     1921 gcgaaagtgg atgctctggc cctggacgcg ggagcaaggg cagtggacag gcaacggctt
     1981 ggcctttatg gtcgcgcgga tgcgtcgtgt ggacatcgaa acgattcatg tgagcctgtg
     2041 gggggaccgg ctggcgcaac ggattggcct gggccaagtg gatgcagagt tcgtgattcg
     2101 cgtgctggaa ccgttcgcca cggaacgtcg gcgggagaag ttgcagcggg tcttcgccga
     2161 ccggatcggt tcggtcacgg tggtgatgga ctcgatttgt gacccccaca acggcgcggc
     2221 attgctgcgg acgtgtgaag cgtttggggt agcgcggatt catttgttgg aacaaggggc
     2281 ctcgtttctc gcccacgcgg gagtcgcccg gggcacgcat aagtgggtcg aggtgcgtac
     2341 ccacgcggac gcggaatcct gtgtgaaagc gcttcgcgac gacgggatgg tgctggtggg
     2401 agcgcatccc caagggaagc tacaagctga ggatttacag tcgattcctc gattggctct
     2461 ggtgctgggc aacgagcatc aagggatacg tccggtcctg acgcaagcat gtgaacacgc
     2521 ggttcgtgtg ccgatgcggg ggtttgcgga aagcctcaac gtgagtgtga gcggagggat
     2581 attgcttgct tatgctacag cccatcgccc cggtgattta ccggaaacgg aacgtcgtcg
     2641 actgtacgca agaggtctgg ctgtcaccgt acccaacgcg ggagcgttgt tggaacgggc
     2701 ggatcatgaa cggttcgcag gcgagcagga caatgcccgg caaggcccgt tcaccgcaaa
     2761 aaatagcctt tcggattgaa gccccacgag tcttttggcc attgcgcggg caaccgcgac
     2821 aatgggccgg tatcgcggat acccaccatc atgtcgtcct ctgcctccag gatcatgaaa
     2881 acgcctcccc tgtggtatgt atgctcgcag ggggcggtct tcggtccgat gaacaccgtg
     2941 acgttgctgc aaggcgtggc ggagggccat gtgcccaccg acagtttggt ccgcgcccat
     3001 gcctggtcac aatggagaaa tattcttcaa atacgtgagg ttagcgcttt tcagaaggcg
     3061 agcaatccct cgttcgaacc acgatgggaa ttgccttacc cagaagacga agggcaagcg
     3121 ttggctcgtt ctttcctacg ctgtgccatc agttccaccg aagcgttgtt gttggcgatg
     3181 catgcggcgg tcgaattgac gcgagcagaa gttgcgatgg tgcatcggta tcgcggcccg
     3241 gaacgtgctt ttgtaaccag tttcacgcat ggtgacggcc ttgaaccgtc ggtaggccat
     3301 gcgttgcctg gttacgatgc ggcttgtgtg gcggcgatgg acggtcgagg ggtgtggggc
     3361 agtcaagggg ttggttcggc ccagcgtgcg ttggtggcgc gtttgagccg cggaa
//
LOCUS       DAOSOR010000620         3404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_27926_length_3404_cov_3.133771, whole genome shotgun sequence.
ACCESSION   DAOSOR010000620 DAOSOR010000000
VERSION     DAOSOR010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27926_length_3404_cov_3.133771"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PK710_22605"
     CDS             complement(<1..285)
                     /locus_tag="PK710_22605"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOT12589.1"
                     /translation="MLKTLIRFCVEHRIAVLLAALGFAAYGIFAYLQTPVESYPDVTN
                     LQVNVIATMPGLAPPEIERQVTIPLERSLNGLPKVLRMRSESLFGLSLVYL"
     gene            complement(324..1427)
                     /locus_tag="PK710_22610"
     CDS             complement(324..1427)
                     /locus_tag="PK710_22610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT12590.1"
                     /translation="MTLSSWILLFPILFLLACSKTPIVQETLNVASSYPQGSIVIPEA
                     SRAFIEVQEVSSEKTAALLRAPARITFRDGAVSKLGAPLPGRVQAVHVRSGDSVKAGE
                     PLVTLDCPDAASSRASLSVARAAQREAQAALQRETHMLEQGVGTEKDRLAAERALAEA
                     DAELVRAQAAARFVGNRLGTTVVLRAPIAGVVVSRNATVGAAVDPGGEPLVEVGDPSE
                     LWVQADIFERDLHLVQPGLRAFLELPTAYEPLAGTVAFVGATIESDARTAPVRINLDA
                     TSHELRPGMFGRVRIETPRKALALPTEAVLIKDGGKSFVHIQTEPGVFVARQVAVSQP
                     VEGKVLITSGIAPGDQVVVRGSLLLDGAAAQLL"
     gene            1545..3041
                     /locus_tag="PK710_22615"
     CDS             1545..3041
                     /locus_tag="PK710_22615"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT12591.1"
                     /translation="MRLTVSLSLWLALATASVLGIHGWFQLQQEESDLDLVARRELSL
                     LATAIRTGVESAVRDGQQPDIEQLLEQLERRDPELDVFVFDKNLALLSSSWGSSPNLP
                     RANAVLRESEPEAIHVQVVEETDFVASTPVRVQGANAGFIVLMRPRAALQTDLSEERQ
                     GLVVSMLAVVIALSLTLSLVVHIRLHRPLHQIMHRVRRLAQGEYSEPIVISGKDEIVE
                     LAREFDLMSQALEESRRKIVQAAESRDRLQAEMAELNRLALVGQLAASLAHEIGSPLQ
                     VLNGRARLLAARQELPSDARRNAEILVEQTDRITDIVERLLSLARRKPLKRHVISLVE
                     PVGQVLELLMPHAERQGVELRWTARDTPPVLANAGLVQQVILNLVQNALHASSRGHRI
                     EVTLQPSTYTPPATSRQENTVCIMVDDEGIGIEPDVLPKVFDPLVSQWREDERRAGTG
                     LGLSVVKSIMTDHGGWVCAAVGPSGRGSRFIVHFPMVDASGRAPTDDD"
     gene            3031..>3404
                     /locus_tag="PK710_22620"
     CDS             3031..>3404
                     /locus_tag="PK710_22620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT12592.1"
                     /translation="MTTKKRLLVLDDDPSVVDYLCESLEAAGYEAVPMTSPREALARL
                     GEEEFDLIITDVEMPEIRGVDLLQAVLESNPSQLVLLITAFGSVEMAVEAVRSGACDF
                     LTKPFNVDFANGRGTSARLRCNR"
BASE COUNT          709 a          911 c         1059 g          725 t
ORIGIN      
        1 gagatacacg agggatagac cgaaaaggct ttcacttcgc atccgaagga ctttgggaag
       61 cccgttgaga gagcgctcca ggggaatggt tacctgacgc tcaatctccg gtggggccaa
      121 ccctggcatc gtggcaatca cgttgacttg caggttggtg acgtcgggat aggattccac
      181 cggggtttgg aggtaggcga aaatcccgta ggcagcgaag ccaagagccg ccaagagcac
      241 cgcgatgcgg tgttcgacac agaagcgaat gagcgttttt agcatatcta tgcgattttc
      301 gaggtgtgta ggctagtgcc gtctcataga agctgtgcag cagcaccatc gagcaatagc
      361 gatccacgca cgaccacctg gtcccctgga gcgatgccgg aggtgatgag cactttgcct
      421 tcgacgggtt ggctgactgc tacctgccgc gcaacgaaga caccgggctc ggtttggatg
      481 tgtacgaagg actttccgcc gtctttgatg agcacggctt cggtgggcag agcgagtgct
      541 ttccgcgggg tttcgatacg aacgcggccg aacatgccgg gacggagttc atgagatgtt
      601 gcgtcaagat tgatgcgtac gggtgccgta cgggcatcgc tctcgatggt agctcccacg
      661 aaagcgacgg ttccggcgag aggctcgtag gcggtgggca gttcgagaaa agcgcgcaat
      721 cccggctgta cgaggtgaag gtcacgctcg aagatatcgg cctgtaccca gagttcggaa
      781 ggatctccca cctcgacgag gggctcgccg ccggggtcca cagccgcgcc gacagtggca
      841 ttgcggctta cgacaacgcc tgcgatgggc gctcggagca caaccgtcgt gccgagcctg
      901 tttccgacga atcgtgcggc tgcttgggcg cgaacgagtt cggcgtcggc ttccgcgagc
      961 gcgcgttccg ccgccaggcg gtccttttcg gtgcccaccc cctgctcgag catgtgggtt
     1021 tcgcgttgga gcgcggcctg agcttcccgt tgagccgcac gagcgacgct caaggatgca
     1081 cggctcgaag ccgcatcggg gcaatcgagg gtaaccaaag gttcgcccgc ttttacggaa
     1141 tcccccgagc gcacatgcac ggcttgcaca cgcccgggca aaggagcgcc aagctttgaa
     1201 accgccccgt ctcgaaaggt gatccgcgcc ggagcgcgaa ggagcgcggc ggttttttct
     1261 gagcttactt cctgcacttc gataaacgct cgagaggcct cgggaatcac aatgctccct
     1321 tggggataac tagacgcaac gtttagtgtt tcttgcacga tgggggtttt cgaacaagcc
     1381 agcagaaata ggatgggaaa taggagtatc cacgatgaaa gcgtcatggc ccagggggag
     1441 agcatctccc atgccacctg tttcccaaag gaaatcgtgg gattgaaggc ccacggactg
     1501 gttcctcttg aaacacttgt atacttttga cacaattgtt tcccatgcga ttgacagtat
     1561 ccctttccct ttggttggcg ttggccacag cctcggtgct tgggatccat gggtggtttc
     1621 agttgcagca agaagagtcg gatctcgact tggtggctcg tcgtgagttg agtttgctgg
     1681 cgacggcgat tcggacaggg gtggagagcg cggttcgcga cggtcagcag cccgacatcg
     1741 aacaattgtt ggaacagttg gagcgccggg atccggaatt ggatgtattc gtattcgata
     1801 agaacctggc tctcctcagc agttcgtggg gaagctctcc caacttgcct cgcgcgaatg
     1861 ccgtgttgcg tgagtcggaa cccgaagcga tccacgtgca agtggtggag gaaaccgatt
     1921 tcgtcgccag cacaccggtt cgtgtccagg gagccaacgc cggattcatc gttttgatgc
     1981 gcccacgagc cgcgttgcaa acggatttgt ccgaagaacg acaaggattg gtggtttcga
     2041 tgctagcggt ggtgattgca ctatccctca ccctgtcgtt ggtcgttcat atccggctcc
     2101 atcgtccttt gcaccagatc atgcaccgcg ttcgacgact ggcacaaggc gaatactcgg
     2161 agccaattgt aatatctggt aaagatgaga tagtagaatt ggcaagagaa ttcgatctga
     2221 tgagtcaggc cctggaagaa agccgtcgca agatcgtgca agccgcggag tctagggatc
     2281 gtctgcaagc agaaatggcc gaactcaatc gtctggcgtt ggtgggacag ctcgccgcca
     2341 gtctggctca tgaaatcggt tctccccttc aagtgctcaa cggccgggct cgtctgcttg
     2401 ccgcgcgaca agaactgcct tccgatgccc gtcgcaacgc cgaaatcctt gtggaacaaa
     2461 ccgaccgcat caccgatatc gtggagcgat tgctttccct tgctagacgt aaaccattga
     2521 aacgacacgt tatttctctt gtcgagcccg ttgggcaagt gctagagctt ctcatgcccc
     2581 atgccgagcg acaaggcgtc gaacttcggt ggacggctcg agacacgccg ccggtgcttg
     2641 ccaacgcggg gttggtgcag caggtgatct tgaatctagt gcaaaacgca ctgcacgcca
     2701 gctctcgggg ccatcgcatc gaggtgacgc tacaaccctc cacctatacg cctcccgcta
     2761 cctctcgaca agaaaacacg gtttgtatca tggtggacga cgaggggatc ggtatcgagc
     2821 cagacgttct gccgaaagta tttgatcctt tggtttcgca atggcgggag gatgaacgtc
     2881 gcgcgggcac ggggctgggg ctatccgtgg tgaagtcgat catgaccgat cacggtggtt
     2941 gggtctgcgc cgcggtgggc ccctctggac ggggaagtcg atttatcgtt cattttccga
     3001 tggtggatgc atctgggagg gccccgaccg atgacgacta agaaacggct cttggtgctc
     3061 gacgacgacc cttcggtggt cgattatctt tgtgaaagtc tggaggcggc gggctacgag
     3121 gcggtgccga tgacgtcgcc cagggaagcc ctggcacgtc ttggagaaga agaattcgac
     3181 ctcatcatca ccgacgtgga aatgcctgaa atccgggggg tggacttgct ccaagcggta
     3241 ttggaatcca acccaagcca gctcgtgctt ttgattactg cgtttggcag cgtggaaatg
     3301 gctgtggagg ccgtgcgttc cggggcctgc gattttttga ccaaaccttt taacgtagat
     3361 ttcgcaaacg ggagaggaac aagcgccaga ttacgttgta atcg
//
LOCUS       DAOSOR010000621         3398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_27996_length_3398_cov_4.531858, whole genome shotgun sequence.
ACCESSION   DAOSOR010000621 DAOSOR010000000
VERSION     DAOSOR010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27996_length_3398_cov_4.531858"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="PK710_22625"
     CDS             <1..1000
                     /locus_tag="PK710_22625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT12593.1"
                     /translation="GAVRLGGEIFGSTGIVDAGKTPVAKDGDTVFTKRNTPVEWMGEL
                     RFALDRQKAGWLGFGAGTRLSAGYGAPDVRVLANLGYAFSLRDTEPGSPHRRYTADRD
                     VEPTYSDRDKDGIPDDLDLCPDDPEDGKEPDPNDGCPAPSDRDGDGIPDDVDECPDEP
                     EDFDGLADHDGCPEDDYDADGIPDVEDKCPREPGVASTNPELHGCPQFIRRIEGSTEI
                     QILKRVEFATNSAVLLPQSFPILDEVYALLKANPDVTKVAVQGHTDNRGGRDLNMKLS
                     QDRADSVMRYLVNKGVVASRLTATGYGFDKPIDTNDTAEGRQRNRRVEFHILEQNN"
     gene            1056..1967
                     /locus_tag="PK710_22630"
     CDS             1056..1967
                     /locus_tag="PK710_22630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOT12594.1"
                     /translation="MNDLLSLLPEVREALRVGAPVVALESTIISHGMPYPTNVQTARE
                     VEEIVRAKGAIPATIALLQGRIHIGLSSEQLDTLGRAKNVRKVSRRDLPIALVSGQMG
                     ATTVAATMFCAHLAGISVFVTGGIGGVHRRGEVTLDISADLMELASTPVAVVCAGAKA
                     ILDLPRTLEVLETHGVPIVGFQTDQFPAFYTRSSGLPVDVRLDDPADVARVMATKWKL
                     GLAGGILVTTPVPEADAMDSREIDEVIEQSLEEANSLSLQGKDITPFLLKKIVERTEG
                     RSLATNVALVKHNADVGAAIAIAYAAQ"
     gene            complement(1970..2620)
                     /locus_tag="PK710_22635"
     CDS             complement(1970..2620)
                     /locus_tag="PK710_22635"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOT12595.1"
                     /translation="MRWTALAFTHLAEILSPSRCAACDSLLDRRALLCAICASTVLRW
                     SGDGNPLAFGHYGGALSSALLRLKYGNRPDLGAALGQLLVPVVLAHLTPRDVDLVIPV
                     PVPYDRLVQRGYNQSALIGRPLASALMARLETRALARKDGSVRQAGLNRSDRLKNLQG
                     AFVLRAPKRVRGKRVVLVDDVRTTGATLQACEDLLLKGGADVVATAVVARTELFES"
     gene            complement(2674..3270)
                     /locus_tag="PK710_22640"
     CDS             complement(2674..3270)
                     /locus_tag="PK710_22640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12596.1"
                     /translation="MTELRRISSFALLAIVLAVGCDRPTPSPQPASSQEPVVGASTSA
                     IASASASVSASATASVPAVVVDPASAKKWSGTYEAKRVELATPPKVTDFTWKNDDGSK
                     AVGNGRLSMIVADGVVGGEASGALGSQVLSGLLDGTLLRLELIPTDPTVSSAMSGGGT
                     GELKDGVISGTMRVAGPKGVEVREVTFKLEPSGQSTAP"
BASE COUNT          752 a         1038 c          933 g          675 t
ORIGIN      
        1 cggcgctgtt cgtcttggtg gtgagatctt cggctctaca ggcattgtcg atgcgggaaa
       61 aactccggta gccaaagacg gcgacaccgt attcaccaag cgcaatacac ctgtggaatg
      121 gatgggcgaa cttcgcttcg cgctcgatcg tcaaaaagcc gggtggctcg gcttcggggc
      181 tggtacacgg ttgagcgcgg gctatggcgc acccgatgtc cgtgtccttg caaacctcgg
      241 ttatgctttt tcgttgcgtg atacggaacc gggctctccc caccgacgat acaccgccga
      301 ccgtgacgtg gagcctacct actccgacag agacaaggac ggcattcccg acgatctcga
      361 cctttgcccc gacgaccccg aagacggcaa agagccggat ccaaacgatg gttgcccggc
      421 cccttcggac cgcgatggtg atggtattcc cgatgacgtg gatgagtgcc ccgacgaacc
      481 tgaagacttc gatggccttg cggaccacga cggttgtccg gaagacgact atgacgcgga
      541 tggaatcccc gatgtcgaag acaagtgccc gcgtgaaccc ggggttgcga gcaccaatcc
      601 cgaactccac ggctgccccc aattcattcg tcgtatcgaa ggctcgaccg aaattcagat
      661 cctaaaacgt gtcgagttcg caaccaacag cgccgtcttg ttgccacaat ccttccccat
      721 tctcgatgaa gtatacgccc tgttgaaagc caacccggat gtcaccaaag tagccgtaca
      781 aggtcatacg gataaccgtg gcgggcgtga cctcaatatg aaactcagtc aggaccgcgc
      841 cgacagtgtc atgcgctacc tcgtgaacaa gggcgtcgtt gcctcgcggt tgaccgccac
      901 cggctatggc ttcgacaaac ccatcgacac caacgatacg gccgaaggac gccagcgcaa
      961 ccgacgggtc gagttccata ttctcgaaca aaataactga tatttcacat acttatgccc
     1021 attcgggcct tggctctccc gacaggacga tgatcatgaa tgacctcctc tccttgttgc
     1081 cagaggtgcg tgaggctttg cgcgttggcg cacccgtcgt tgccctcgag tcgaccatca
     1141 tctctcatgg aatgccgtat cccaccaacg tgcagaccgc gagggaagtc gaagaaatcg
     1201 ttcgcgcaaa gggagcgatc ccagccacca ttgccctttt gcaaggccgc attcatatcg
     1261 gcttgtcttc cgaacaatta gatacgctgg gccgtgcgaa aaacgttcgc aaggtgagtc
     1321 gtcgtgacct gcccattgct ctggtctccg ggcaaatggg agccacgacg gtggctgcga
     1381 cgatgttttg cgcccatctt gcgggcatct ccgtatttgt gaccggaggc atcggcggcg
     1441 ttcatcgtcg tggtgaggtg acgctcgata tctccgcgga cttgatggaa ttggcctcga
     1501 cacccgtggc ggtggtatgc gcgggagcca aagcgattct cgatttgcca cggacgctcg
     1561 aagtgctgga aacgcacggc gttcccatcg ttggatttca aaccgatcaa tttcccgcgt
     1621 tttacacgcg atccagcggg cttccggtcg atgtgcggct cgatgacccg gccgacgttg
     1681 cgcgcgtcat ggccacgaag tggaaactcg gtcttgccgg tggaatcttg gtgaccacac
     1741 ccgttcccga agcggatgcc atggactccc gtgagatcga cgaggtgatc gaacaatcct
     1801 tggaggaagc caacagcctg tccctgcaag gcaaagatat cactccgttt ttattgaaaa
     1861 aaatcgtgga acgcaccgaa ggtcgcagct tggctacgaa tgttgcgctc gtcaaacaca
     1921 acgctgacgt aggtgctgcc attgccatcg cctatgcggc tcaatgagcc tatgactcga
     1981 ataactccgt tcgtgccacc acggcggtgg cgaccacgtc ggctccccct ttcaacaaca
     2041 aatcctcaca cgcttgcagt gtggctccgg tggttcgcac atcatcgacc agcacaaccc
     2101 gtttgccccg cacacgcttc ggcgctcgaa gcacgaaggc cccttgcagg tttttgagac
     2161 gatccgagcg attgagccca gcttgccgaa ccgatccatc ctttcgagca agcgcgcgcg
     2221 tttcgagacg agccatgagc gctgaggcca aagggcggcc gatcagggcc gattgattgt
     2281 agcctcgttg tacgagacga tcatacggaa caggtacggg aatcaccaaa tcgacatcgc
     2341 gcggagtcag atgagctagc acgacaggaa cgagaagctg gccgagggca gctccaaggt
     2401 caggacgatt cccatacttc aagcgcagca aagctgagga aagggcgccg ccataatgac
     2461 cgaacgccaa agggttcccg tcaccggacc atcgtaggac cgtggatgca caaatggcgc
     2521 acaacaacgc gcgtcgatcg agcaacgaat cacaagctgc acaacgcgaa ggggatagga
     2581 tttcagcaag atgagtgaac gcaagagctg tccatcgcat accccttttc ggcccaaacc
     2641 accgcctggt tgcgccctat tcacaaggca aaattacgga gctgtgcttt ggcccgatgg
     2701 ttccagcttg aacgttacct cgcgtacctc cacccctttg ggaccggcaa ctcgcatggt
     2761 gcccgaaatc acaccgtctt tgagttctcc tgtaccacct cccgacatgg ccgacgacac
     2821 cgtgggatcg gtcggaatca attccaatcg caacagggtg ccatcgagca aaccagatag
     2881 cacttgagaa cccagcgcac cgcttgcctc acctcccacc acgccgtccg ctacgatcat
     2941 cgatagccgt ccattgccga ccgctttcga accgtcgtca tttttccatg taaaatcagt
     3001 aactttgggt ggtgtggcca gctccacgcg tttggcctca taggttccgg accacttctt
     3061 ggcagaggcg ggatcgacaa cgacagcggg caccgatgcg gtggcagacg ccgataccga
     3121 agcgctcgcg gaggcgatgg cggaggtgct ggcgcccacc actggttctt gcgaagacgc
     3181 tggttgtggc gacggcgtgg gtctgtcgca tcccacggcc aaaacgattg ccaacaaggc
     3241 aaagctcgat atccgtcgca attccgtcat gccgcgccat ccttgggagt cataagttcg
     3301 tgaatccgta ccccaagctc tccttctacg tccaccaatt cgccccgggc aacctcgatt
     3361 cctccgaccc gaagacagat tggctgtcca aggcttcg
//
LOCUS       DAOSOR010000622         3385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_28120_length_3385_cov_4.993694, whole genome shotgun sequence.
ACCESSION   DAOSOR010000622 DAOSOR010000000
VERSION     DAOSOR010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28120_length_3385_cov_4.993694"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..316)
                     /locus_tag="PK710_22645"
     CDS             complement(137..316)
                     /locus_tag="PK710_22645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12597.1"
                     /translation="MSRWDAETRVVHTVSGIELLKKAYPDYSRLPKELAVLKAPKQAL
                     YPKGSLNPGGIFDNG"
     gene            complement(1473..2003)
                     /locus_tag="PK710_22650"
     CDS             complement(1473..2003)
                     /locus_tag="PK710_22650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12598.1"
                     /translation="MKHNTVSHAIDTHGAEYPPRRKPVCHPCYDPDPDNPGTEGAIVR
                     DNVIDNPDCSGSAITLRGGKGLVQHHTIRGYSKAIQLSKQTPQVIEPIPIWDDDIIDP
                     MQWITGAGKRIPAIASLRLGLRGFRHGFRQARKRTRHVRGLGHRRPLWLAPTLLPAAL
                     VIVGPGHDMLTPSQRA"
     gene            complement(2137..2634)
                     /locus_tag="PK710_22655"
     CDS             complement(2137..2634)
                     /locus_tag="PK710_22655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12599.1"
                     /translation="MKRSSTSYRIAVAATMFAHSAHAQTVLFASTGTQADWQAAIDIV
                     ESPAVIVIPSGKWQIVESIDLTKDANTIIGAGMYRTRLSRDARLPTPTPPRSPNTKSL
                     SSARRQKVRFVFLRLGSRVGSPPASKTGRSAFLFRTYRTFVWTLAPSVSREVVASKLG
                     ESSGA"
     gene            2730..>3385
                     /locus_tag="PK710_22660"
     CDS             2730..>3385
                     /locus_tag="PK710_22660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOT12600.1"
                     /translation="MKESPSRREALKRIGATAAVAVGAGALATLRWDRHGQAREASSQ
                     RHAIKDFRVPPRTDLPTMAVAKRGTDPNSLTRAAIDALGGMSRFVAKGDVVTIKPNVG
                     WDRSPMHAANTNPEVVASVVRACYEAGAKHVIVTDYSCNEVQRSFQRSGIWKAAYEAG
                     AEVLLPAEHQFRTVAVGGTVLGEWPVLRPIIDADKVINVPIAKHHSLARFTGCMKNWY
                     G"
BASE COUNT          689 a          939 c         1024 g          733 t
ORIGIN      
        1 tcaccggtcc gctgttcgaa gacacggccg acgcgggaca gtagacagcg gtgtcgccgc
       61 aggaaacgtc agatccatca tcaccacggc agtcacgaga caacatggcg agcttgctct
      121 gtagcagttc gccgtcctac ccgttgtcga agatcccccc cggattgagg ctccctttcg
      181 gatacagtgc ttgtttgggc gctttcagta ccgcgagttc cttcggcaag cgcgaatagt
      241 caggatacgc ttttttcaat agctcgatgc cggaaaccgt atgtacgaca cgtgtttcag
      301 catcccatcg gctcacccga cctcggcctc acaagccatg gcgggtcgcg aacggatgaa
      361 atacccaagg gggcagcagt acccctctgc ggatggaaag ggcaccagtt cttgcgcctc
      421 tcgcgagccc aaggctcgct cacgcacgcg attttgcccc acaattgggc ttcgatccgg
      481 tcttgttcga ggcgttgctg ctgggcgggg cgggcagggc agtgggggcg ttcgccgcga
      541 gtaaaggcgg cgacgcatgg gtttggctcg agatcccttt gccaccacgg atgactgctg
      601 gttttgtgct ctttacatga tcgcacgggc ggttgagctc gttgacgttc gatttgtttt
      661 gcgagaggcg ttggaatttc cgatcggcgt ccgtcgtttc agcacctaat cctgtgcagt
      721 tatcctgcag tgcatggagt ttttcggcgc ttttcttcct aatctcccta cttgcggcga
      781 cgccgcgcgc attgaccaac gcttccaatc cccatcaacc tctttctgcc ccagcggttc
      841 tggccgagtg gtgatgggta tgacttttcg gcagtcgata acaagggaaa tgctccggcg
      901 aaaatgaggg gatggctctg atttgctgtg ctttgcagac agcctcttcg acaatcccac
      961 catcagcgat atcctgacca aaccctcatc tggaaagggg ctttggatca tggatcccct
     1021 attcgcggaa aacctttgcc aactgctcac agtctatcgg aagaacaaca gattcaacca
     1081 cacccactct cgctgatacg ttgaatcgac tcggggctga taccacaagg cgaaatcttc
     1141 aaaaaactcg ttggaagtgg ggagcctcct ttggatgaga gacgtgcttt ggtgcgatga
     1201 gttcgattca cgcgagattc gcgagacgag gcaaggcctt ttgcgcggac aacaacggcg
     1261 atagtctggg aaatatatgc cgatacgtgc ttcgtcatcc tatcggcttg tcgcgcctca
     1321 gcctcataag cgatgggtgc ggcgcgtacg agatgaagta cccaagaggc agcaggactc
     1381 ctctgctgat ggaaagagta ccatttcttg ctcatctagc gtctctggtg cccccaccgc
     1441 gtcacccgtt ggtctggtac gtaggcgtgt tgtcaagcgc gctgcgatgg cgtgagcatg
     1501 tcgtgcccag gtccaacgat gacaagggct gccggcaaca acgtcggagc gagccacaag
     1561 ggccgtcgat gaccaagacc acggacgtgt cgtgtacgct tgcgggcttg cctgaagcca
     1621 tgcctgaagc cgcggagccc caagcgcagc gaagctatcg cggggattcg ctttcctgcg
     1681 ccggtgatcc attgcatggg atcgatgatg tcgtcgtccc agataggaat gggctctatt
     1741 acctgtggcg tctgtttcga gagttgaatg gccttggaat aaccgcggat cgtgtggtgc
     1801 tggacgagtc ccttgccgcc gcgcagggta atggccgagc cgctgcagtc ggggttgtca
     1861 atgacattgt cgcgaacgat ggccccttcg gttcccggat tatccggatc cggatcatag
     1921 cacggatggc agacgggttt gcgacgggga gggtattccg cgccgtgggt atcgatggcg
     1981 tgggacacag tgttgtgttt cacggcgtag tgccggaata cgtaacgcgc tccttgccat
     2041 gggcttctcc catgggaacg gttccaccta cggaaacccc atagccacaa ttaccgatgc
     2101 tggcttcgta tctatcccga aatgtataat cgttgattac gccccggaac tttcccccaa
     2161 ctttgacgcc accacttccc gtgaaacgga aggggcaagg gtccacacga aagtccgata
     2221 cgttcgaaaa agaaatgccg accttccggt cttcgaggct ggtggactgc caactcgtga
     2281 ccccaaacga agaaagacga accgaacttt ttgccttcga gcggaagaaa ggctcttggt
     2341 attgggtgag cgtggcggcg tcggcgttgg caggcgagca tccctactca atctggttct
     2401 atacatcccc gcgccaatga tggtgtttgc gtccttcgtg aggtcgatgc tctccacgat
     2461 ttgccatttg ccagagggga tcacgatcac ggccggtgat tcgacgatat cgatggcggc
     2521 ttgccaatcg gcttgtgttc ccgtggaagc aaagagcacg gtttgggcgt gggcggagtg
     2581 agcgaacatc gtcgctgcga cggcgatgcg ataggaagtc gatgagcgtt tcatgcggtt
     2641 ggtggactcg acgtgtatgg tgcgtatcca cgatcgcaag aggcattccg ggcttgcgcg
     2701 agcgcacaaa gaaccgtaac gacaaagcca tgaaggaaag tccatcgcga agagaggcgt
     2761 tgaagcgcat cggggcgacg gctgccgtgg ctgtgggcgc cggagccttg gccacgttgc
     2821 gatgggaccg acacggccaa gcgcgggaag cctcgagcca gcggcatgcg atcaaagatt
     2881 ttcgtgtgcc gccgcggacc gatttaccca ccatggccgt ggcgaaacga ggcaccgatc
     2941 cgaattcgtt gacccgcgcg gccatcgatg cgttgggcgg gatgagtcgg tttgtggcca
     3001 aaggagacgt ggtcaccatc aagccgaacg tgggctggga ccgttctccc atgcacgccg
     3061 caaacaccaa tcccgaggtg gtcgcctccg tggtacgtgc ttgttatgaa gcaggtgcca
     3121 aacacgtgat tgtcacggat tactcctgca atgaagtgca gcgttccttt caacggtccg
     3181 ggatttggaa ggcggcttac gaagcaggcg cggaggtctt gctccccgcc gaacatcagt
     3241 ttcgaacggt ggccgtcggt ggcaccgtgc tgggtgaatg gcccgtgctt cggccgatca
     3301 tcgatgcgga caaggtcatc aacgtaccga ttgccaagca ccacagcctg gcccgcttca
     3361 ccgggtgcat gaaaaattgg tacgg
//
LOCUS       DAOSOR010000623         3381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_28158_length_3381_cov_4.361395, whole genome shotgun sequence.
ACCESSION   DAOSOR010000623 DAOSOR010000000
VERSION     DAOSOR010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28158_length_3381_cov_4.361395"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..405)
                     /locus_tag="PK710_22665"
     CDS             complement(115..405)
                     /locus_tag="PK710_22665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12601.1"
                     /translation="MMANDSERNHPRTLPVWEQPRGDEACTTCQSQTWIWEEEVHLVR
                     QMIETKYKARQARRAGDANAVHALRQQFESLRQQMEKVRRQRLDSLGYTDYD"
     gene            484..903
                     /locus_tag="PK710_22670"
     CDS             484..903
                     /locus_tag="PK710_22670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOT12602.1"
                     /translation="MKSFRKELWFETKSRREYINITQRVREALRESGIREGLCLVNAM
                     HITASVYINDDESGLIQDYDDWLEKLAPHEPTSHYRHNRTGEDNGDAHLKRQVMGRQV
                     VVAVTDGDLDFGPWEQIFYAEFDGRRRKRALVKIIGE"
     gene            complement(907..2088)
                     /locus_tag="PK710_22675"
     CDS             complement(907..2088)
                     /locus_tag="PK710_22675"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOT12603.1"
                     /translation="MQALRVQARRVAWHAALLVTFLSLHALARPAVDMSGARRRGGHW
                     TAPTSQGAAVLTLDPHLQKDVETLLRQARAPEAAAVVIDVRTGRILAWTSVDGQGRDL
                     VSEPYAPPASLFKVVTAAALLEDKVPVSARQCYVGGHRNVHLRDLRSSGAGGATCSTF
                     SEALGYSLNMVMAGLANRYLDAKKLQAFALRMGFNGSVPIDVEVRGGSARVPTSEEGM
                     ARAAAGFGPGTLTPLEAAYMMTIIARDGERPALQLIDGWVDSDKALRPTPVSDRRAIG
                     AATARTLRGLLEVTPREGTAFKAFRDPSGKRYLGSHGGGGKTGTLTRGQQSRLFSWYA
                     GFAPAQNPQVAVAVMLANEQRWWRKGSEVARDVLRAYFAHQKTAGITHPLRSSTARRS
                     P"
     gene            2234..>3381
                     /locus_tag="PK710_22680"
     CDS             2234..>3381
                     /locus_tag="PK710_22680"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOT12604.1"
                     /translation="MRSISLTLCLGLAVALGCGSPNNPLRIPPPPAMPEPTPTSQPLP
                     DPPKKTPTADVDPVASGLTVRGIQSLCDEHLERAQSLLESIQSLDNAPAQSLNWDNTI
                     GRYDEIVLELSVAGGFPSLMALGHPDKTLRDAAKDCEPKIEKFRTDLMLDTRFATVIR
                     RYADQKPPLQSVRERLLRELLRDLRRYGLELPADAQARLRKINEELSKLQQDFSSNIS
                     DAVLTIQVKPAQLRGLPDSFVEQHKPGPDGLVTLNTNYPDYFPVVTYCEDRTVARDLT
                     AKFDSRAADKNLSILDRVLALRNEKAKLLGYETWADYAIEPRMAKTAHTVRDFLDRLS
                     HELQEPARKEYQEFAAEYRRLGHNPGAAIPITNACTSNKNCENANMGST"
BASE COUNT          791 a         1124 c          807 g          659 t
ORIGIN      
        1 ggacaatgat aataaattaa cagaatgccc ttttaaaaga aattcatcaa taaaatcaac
       61 atattactca cttctccctc gagaacgaac gaagcgttcg ccgccaccac gacatcaatc
      121 gtaatccgtg tagccaaggc tatcgagtct ttgccgtcgt actttttcca tctgttgtcg
      181 cagcgattcg aattgttgcc gcaacgcatg gacagcgttg gcatcgcctg cccgacgcgc
      241 ttgccttgcc ttgtatttcg tttcgatcat ctgacgaacc agatgcactt cttcttccca
      301 aatccacgtt tggctttggc acgtggtgca ggcttcatca cccctgggtt gttcccacac
      361 gggaagggtt cgcggatggt ttcgttcgga atcgttagcc atcatgccaa tccttggatg
      421 cattgacgaa gccaggggtc tcgctgcatg gtcttggcct cgttttcaaa aaagggagcg
      481 ctcatgaaat cctttcgcaa ggaactttgg ttcgaaacca agtctcgcag agagtatatc
      541 aacatcaccc aacgggtccg cgaggcattg cgagaaagcg gtattcgcga ggggttatgc
      601 ctggtcaacg ccatgcacat cacggccagc gtatacatca acgatgatga atcgggcttg
      661 attcaagatt acgacgattg gctcgagaag ctcgcccccc atgaacccac atcgcactat
      721 cgtcacaacc gcacggggga agacaatggc gatgcccatt tgaaacgcca agtcatgggc
      781 cgacaggtgg tggtagcagt caccgacggg gacctcgatt ttggcccctg ggagcagatt
      841 ttttacgcgg aattcgatgg gcgccgacga aagcgcgcac ttgtcaaaat catcggcgaa
      901 taacgcttat ggtgagcgtc gggcggtgga cgatcgaaga ggatgcgtga tgcccgccgt
      961 tttttgatgg gcaaagtaag ctcgcagcac gtcacgggct acttccgacc ctttgcgcca
     1021 ccatcgctgc tcgttggcga gcatgacggc taccgcgact tgtggatttt gcgcgggggc
     1081 aaaccccgcg taccatgaaa acagccgcga ttgctggccg cgcgtcaacg ttcccgtctt
     1141 cccaccaccc ccatgagatc ccaaataccg ttttcctgat ggatcacgaa acgctttgaa
     1201 cgccgtgcct tcccgcggtg tcacctcgag caaaccacga agggtccgag ccgtcgcagc
     1261 cccaatcgca cgacgatcgc ttacgggagt cggacgcaac gccttgtcgg aatccaccca
     1321 gccatcaatc aactgtagcg ctggacgctc cccgtcacgc gcgatgatcg tcatcatata
     1381 agccgcttcc agcggtgtca aagtaccagg accaaagcca gcggctgccc gagccatacc
     1441 ctcttcactc gtgggtactc tggccgagcc acccctcacc tcgacatcaa tcggcaccga
     1501 tccgttgaac cccatccgca acgcaaaagc ttgtagtttt tttgcatcga gataccgatt
     1561 cgcaagcccc gccatcacca tgttcaagct gtaccccaac gcttcggaaa acgtggagca
     1621 agtggctccg ccggcacccg acgatcgaag gtcgcgcaag tgaacgttgc gatgcccccc
     1681 gacataacac tgccgcgcgc ttacgggcac tttatcctcc aatagcgcgg ccgcggtcac
     1741 cactttgaat agactcgcgg gtggcgcata gggttcggac accagatcgc gcccctgccc
     1801 gtccacactg gtccatgcca aaattcggcc cgtgcgaaca tcaatcacaa cggccgcggc
     1861 ttccggcgca cgggcctgcc gcaacaatgt ttctacgtct ttctgtagat gcgggtccaa
     1921 ggtcaacacc gcggccccct gcgaagtcgg agccgtccaa tgcccacccc tgcgccgtgc
     1981 ccccgacata tccaccgcgg gacgagccaa ggcatgcagg ctcaaaaagg tcaccaacag
     2041 ggccgcgtgc cacgcgacac gccttgcttg gactctcaac gcttgcatca ccttttcacc
     2101 taggacggca tcgtccaatg ggccaaattc cttggcaaat tccacgcccc atcgcctcga
     2161 tcgctatgat accgttcaat ttccgtgcaa accggggttt gtcggcaccc acttccgagg
     2221 tatgaccacg cccatgcgct cgatatcact caccctttgc ctgggattag cggtcgcgtt
     2281 gggatgcggc tcccccaaca accccctgcg aatcccacca cctccagcca tgccagaacc
     2341 cacgcctact tcccaaccac tgcccgaccc ccccaaaaaa acgccaacgg ccgatgtcga
     2401 cccagtcgct tccggattga ccgtacgagg aatccagtcg ctgtgtgacg agcacctcga
     2461 acgcgcccaa tcccttctcg aatccatcca atcgctggac aacgccccgg cgcaatcgct
     2521 gaactgggac aataccatcg gacgatacga cgaaatcgtg ctcgagctat ccgtcgcagg
     2581 cggcttccct tcgctcatgg cgcttggtca tcccgacaaa accctgcgcg atgccgccaa
     2641 agactgcgaa ccaaaaatcg aaaaattccg caccgacctc atgctcgata cgcgctttgc
     2701 caccgtcatc cgccgatacg cggaccaaaa accacccctg caaagcgttc gagaacgatt
     2761 actccgcgag ctgctccgcg atcttcgacg ctacgggctc gaactgcccg cggacgcaca
     2821 agcccgcctg cgcaaaatca acgaggaatt atcaaaactc caacaggact tctcctccaa
     2881 tatctccgat gccgtgctca ccattcaggt gaaacccgcc caactccgag gcttgcccga
     2941 ctccttcgtc gaacagcata aacccggccc cgacggactc gtcaccctca acaccaacta
     3001 ccccgactac ttccccgtcg tcacctactg cgaagaccgc accgtcgccc gtgacttgac
     3061 tgcaaaattc gacagccgcg ccgcagacaa aaatctgtcc atcctcgacc gagtcctcgc
     3121 cctgcgaaac gaaaaagcca aactcctcgg ctatgaaacg tgggcggatt acgccatcga
     3181 acctcgaatg gcgaaaaccg cacacaccgt acgtgatttc ctggaccgct tatcccacga
     3241 attgcaagaa cccgcccgca aagaatacca agaatttgcc gcggaatatc gtcgcctcgg
     3301 acataacccc ggcgccgcca tcccaattac gaatgcttgt acatcgaaca aaaactgcga
     3361 gaacgcaaat atgggttcga c
//
LOCUS       DAOSOR010000624         3367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_28321_length_3367_cov_3.689614, whole genome shotgun sequence.
ACCESSION   DAOSOR010000624 DAOSOR010000000
VERSION     DAOSOR010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28321_length_3367_cov_3.689614"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="PK710_22685"
     CDS             <1..793
                     /locus_tag="PK710_22685"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOT12605.1"
                     /translation="GAQQMQRVEVRFGQGQRQILRRRGPGFEMLEPKGYAVDAALAAD
                     LFDTVSHLRAERWVAERDDGSFGLGTPSLIARMTFDEQGKEQTRSLRLGTETQQGRYG
                     RWDPDTGVFVLSRATEKALQTYAIDRLVFMVDPSEVHLLRLEAGTKDVWIGVTGDSWR
                     SQRGSMHPMDEATIAKIRQLLSELRAEGVVTLGAAKPEQGFAKPQLRVEVQSVLRKEK
                     IAFSIGYGDVWRSMNVVYVRRDGVEATYAIAQSKVLPLIEAMGGP"
     gene            837..3074
                     /locus_tag="PK710_22690"
     CDS             837..3074
                     /locus_tag="PK710_22690"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOT12606.1"
                     /translation="MRTSHDFPRQFSLLGRSLRIRNIRPGKRLITPKTSLAILVALSI
                     VASASTAAAITRCEVLRRAQAWVDDPNAWYSWDPVYTDPTTGVCCYRPDCSGLVSAAW
                     GLPPPGHTTYSFAPGPWNDGVSFVISPAELKPGDALNYPGNPNAGTGHIMLYVSGNFS
                     SGYVEVMEEYDYGHTAERRWRNIDTSAYYPIRYVGIVDGEPEVCNGIDDDCDGLIDEG
                     VCGQDYLLRGQAWIAPARTTDIDGDGRADVCGRGMSGVWCHLSKGASWSEKGPVLSMS
                     DADGWNDPMNWGTMRYGDIDGDGRADLCVRADIGVQCWKSDGVGLRIEVSGPPWSDDN
                     GWGHAPFLTTMRLMDFDGDGKDDLCARASKGIECYSSTGNGFGPKVEGPAWSDAAGLN
                     EPKYYGTLREGDVDGDGRVDVCMRTQQGMECWLSDGKGFPTRVQGPSWADEDGWGELR
                     YWSTIRLADINGDGKADLCARDSQALRCYFSQGNGFASGVEVAPLSDAMGWDDETNFL
                     TIRTGDFDGDGRQDLCARSNSVIECYRWDGATFVRVDGPAWSDGDGWNQSQYFDTIQM
                     GDMDGDGRADICGRHVDGWRCHLSEGTGFGPAIVVDEFTDQGGWGQPKYYSTLFFGGP
                     GCHPSEEICNGKDDDCDGLVDEEGVCEQGGGVDSGLGGGGSGGLMGSGGSGGSKAPDG
                     GHGGVGSQAIKGEESTGGSLYQPPASGCACRTGAWAARGRFVVGLMMGMVGVLLARRR
                     KHETP"
BASE COUNT          666 a          778 c         1189 g          734 t
ORIGIN      
        1 gggagcacag cagatgcagc gggtcgaagt gcggttcggc caaggacaac gtcagattct
       61 gcgtcgacgg ggacctggat tcgagatgct cgaacccaaa ggctatgcgg tggacgccgc
      121 ccttgccgcg gatttgttcg atacggtgag ccacctgcga gcggaacgtt gggtggccga
      181 gcgcgatgat ggctcgtttg ggcttgggac cccttcgctc atcgcacgaa tgaccttcga
      241 cgagcaaggg aaggaacaaa cacgttcgtt gcggctgggc acggagacac aacaggggcg
      301 ctatgggcgt tgggatcccg acacgggagt attcgtgctt tcccgagcca cggaaaaagc
      361 gttgcaaacg tatgccatcg accgattggt gttcatggtc gatccttctg aagtgcattt
      421 gctgcgcctc gaggcaggaa cgaaagacgt ttggataggc gtgacgggcg attcttggcg
      481 atctcaacga ggaagcatgc atccgatgga cgaggcgacg atcgcgaaaa ttcgtcaatt
      541 attatcagag cttagagctg aaggggtcgt gactctcggc gcggcgaaac cggaacaagg
      601 gtttgcgaaa ccccaattgc gtgtcgaggt gcagtccgtc ctccgaaaag aaaagatagc
      661 gttttccata ggatacggcg acgtctggcg ttcgatgaac gtggtatacg tgcgtcgtga
      721 tggggtggag gccacgtatg cgatcgcgca atcgaaagtc ctgccgctca tcgaggccat
      781 gggagggcct tgagggtcgc gccggagtgg ttcgaaaaat gcttatccaa gggagcatgc
      841 gaacttctca tgactttccc cgacaatttt cgctccttgg gcgcagcctt aggatccgca
      901 acatccgccc tgggaaacgg ctcatcacac cgaagacttc ccttgccatc ctcgtggcgt
      961 tgtcgatcgt ggcgagcgca tcgactgccg cggccattac acgttgcgaa gtattacgcc
     1021 gggcacaggc ctgggtcgat gaccccaacg catggtattc atgggatccc gtctacacgg
     1081 accctaccac tggggtttgc tgctaccggc cggattgttc cgggttggta tcggcggcct
     1141 ggggcttgcc gcctccagga cataccacct acagtttcgc gccagggccg tggaacgatg
     1201 gcgtttcttt cgtcattagc ccggccgagc taaaacccgg cgacgctctc aactatcccg
     1261 gcaatcccaa cgccggcacc ggacacatca tgctctacgt gtcgggcaac ttcagcagtg
     1321 gatatgtcga ggtgatggaa gagtatgatt acgggcatac cgccgagcgt cgctggagaa
     1381 atatcgacac ctcggcctat tacccaatcc gttatgtcgg gattgtcgat ggtgaaccgg
     1441 aagtatgtaa cgggatcgac gatgattgtg acgggttgat cgacgagggg gtgtgcgggc
     1501 aggactacct attgcggggg caggcctgga ttgccccggc gcgcacgacg gatatcgatg
     1561 gcgatggtcg tgcggatgtg tgtgggcgcg gaatgtccgg tgtttggtgc cacctatcga
     1621 aaggggcttc gtggagtgag aaaggacctg tcttgtccat gtccgacgcg gatggttgga
     1681 acgacccgat gaattggggc acgatgcgct atggggacat cgatggggat ggccgtgcgg
     1741 acctttgcgt tcgagcagat atcggtgtgc agtgttggaa atcggatggg gtgggcttgc
     1801 gtatcgaggt gtcagggcct ccgtggtcgg acgacaatgg ttgggggcac gcgccatttt
     1861 tgaccacgat gcgtctcatg gacttcgatg gggatggcaa agacgatctg tgtgcgagag
     1921 cgtccaaggg catcgagtgt tattcatcga cgggaaacgg ttttggtccg aaggtggagg
     1981 gaccggcgtg gtccgatgcg gcggggttga atgaaccgaa gtattacggg acgttacgtg
     2041 agggggacgt ggatggggac ggacgcgtgg atgtatgcat gcgcacgcaa cagggcatgg
     2101 agtgttggct ttcggatggc aagggatttc ccacgcgcgt gcaaggaccc tcctgggcgg
     2161 atgaggatgg gtggggagaa cttcgatatt ggagcacgat tcggcttgcg gatatcaatg
     2221 gggatggaaa agcggattta tgcgcgcgcg attcccaagc gcttcgttgt tacttttccc
     2281 agggcaatgg gtttgcgagc ggcgtggagg tagcgccgtt gtcggatgcg atggggtggg
     2341 acgacgaaac caattttcta accattcgaa ctggggattt cgatggagat ggcaggcaag
     2401 acctatgcgc tcgatcgaat tccgtgatcg aatgttatcg atgggacggt gcgacgttcg
     2461 tgcgtgtgga tggacctgct tggtcggatg gggatgggtg gaatcaatcg caatacttcg
     2521 acacgattca gatgggcgat atggacggcg atggtcgcgc ggatatttgt ggccgtcatg
     2581 tagacggttg gcgatgtcat ctgtccgagg gaacggggtt tgggccggcg attgtggtcg
     2641 atgaatttac cgaccaggga ggttggggtc agccaaagta ctattcgacc ctattttttg
     2701 gcggcccggg ttgccatcct tcggaggaga tctgcaacgg caaagacgat gattgtgatg
     2761 gtttggtgga tgaggagggt gtgtgtgagc aagggggagg ggtcgatagt gggttgggcg
     2821 gagggggaag tggcggtttg atgggaagtg gcggttcggg tgggtcgaaa gctcccgatg
     2881 gtggccatgg tggggtgggc tcgcaggcga tcaagggaga ggaatccacg ggaggttcct
     2941 tgtatcagcc tcccgcttcg gggtgtgcgt gccggacggg cgcatgggcg gcccggggtc
     3001 gattcgttgt gggcctgatg atggggatgg taggtgtcct gctagcaaga aggagaaagc
     3061 acgaaacccc ctgacgcggg tgggctgtcc ggtcgtgccg cgaacgtggt ccgcctggtt
     3121 ttgagcgggt ctttggtgct tgcggccggt cgtggggtcg atgttcggcg catttccgtg
     3181 gttcgacgaa ttgaaatagt gtgtaatttc gataggttgg tgcttatccc ccaagacgga
     3241 tccttcgcag cggcaacgaa cactagcggc tgatcatcgg cgccatgagg tgttggatcg
     3301 gttgcgaaag atgggcagca gcttgtcggc ataggcaacg tttggtgcta acctcggatg
     3361 gaccaga
//
LOCUS       DAOSOR010000625         3363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_28348_length_3363_cov_3.467352, whole genome shotgun sequence.
ACCESSION   DAOSOR010000625 DAOSOR010000000
VERSION     DAOSOR010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28348_length_3363_cov_3.467352"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2298
                     /locus_tag="PK710_22695"
     CDS             <1..2298
                     /locus_tag="PK710_22695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12607.1"
                     /translation="FLAISLGMFGCGSDEQSSPGVSGSGGSAGNANGGSGGAAVDGGS
                     GGTANVGGAAGMGGNGGTGGGNPLEPPVEASNQIIMRNQSPQTLTNYPFQFGRPFLQG
                     EIKAFPVVYANDTPIPTQTDIKQRWPDGSVRFAIVSVVIDALEPNATMTLKFGEQSTS
                     DNTPLTTAEMLDPVFDFDAQMALTSGGQTVSASARKMLTDGKAIPWVSGPIATTVILA
                     DHSKGRVYDLGFDTYRSIRPIFEATFWPRTHQVRIRFIGENANTTTLQDVAYDLALTT
                     GSTAPKSFYTRANVPHYFGTRWTKVAWIPTAPESNVLIDFGLPYLAQSQFMPNFDTSR
                     TVPEERLAALAASWSAADKELFDTGMWTPYMPTTGGRAEIAPYPSWTNLWLRTGDPRA
                     REAALGQADLAGSWPLHWREGDEQTKFDRKKTIPGLGMPISVNAHPDIWFPDNNGNYH
                     GILPTPRISPKTPWVADGAHQPDPFSPQYILTGDHFYLEQIQLWAAAQTVAYPYGQYG
                     RGLNGYGGIQDQIRGNGWVIRNRVNAAFLSPDGTPEKTYFHELIEDAFALWEGQRNIQ
                     GTPLANHPQWAYGHDTIPVTASPLHIFDRGIDLASTSLNGLDPNTTSRITALWQHYYF
                     LIELGVARDRGFPAEPLLKWLAPVLNGQFTDPGYDPVLCRMYMTPIADVSGNYFPTWA
                     DIANAIPQSTYEAQKTAGGSNYYPLLAASAASATYWEPGGEEAWAWLSTHVRATSQTD
                     YGTEWFSWDLLPRPEGTKPLPPSTP"
     gene            complement(2682..>3363)
                     /locus_tag="PK710_22700"
     CDS             complement(2682..>3363)
                     /locus_tag="PK710_22700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12608.1"
                     /translation="EEPTTEEIVVDNRITAINSVGYGDAAAESGTTTPAPAFTHSNPY
                     LPAETGGDDDLDLLHAAQKRRRIVRVLVLVIPVLLTLLILLFALANGAFSSNAGLPPP
                     LPTTIPTEPTSRNDDSDRVPEPSTPSKPSMPSAGTDQAVLVVRGPPTGMVYVMGKPLG
                     ATDSPILTDCGQRFVRVGSEMGAKGLMSVQWLAKGQSVQLRCGETTTITADPSVRAES
                     AGEEGRVP"
BASE COUNT          711 a         1007 c          920 g          725 t
ORIGIN      
        1 tttctggcaa tttcgttagg gatgttcggt tgtggatccg atgagcagtc ctcccctgga
       61 gtctctggtt ccggcggcag tgcgggaaat gccaacggtg gctccggcgg ggctgcagtc
      121 gacgggggca gcggagggac tgccaatgtc gggggagccg ccggtatggg aggtaacggt
      181 gggacgggcg ggggaaaccc gctggagccg cccgtggaag cctcaaacca aattatcatg
      241 cgaaaccaat ccccgcaaac gctcacgaac tacccattcc aattcggtcg tccctttctg
      301 caaggcgaga tcaaggcatt tcctgttgtt tacgccaatg acacgcccat cccgacccag
      361 acggatatca aacaacgctg gcccgatggt tccgtccgtt ttgccatcgt gtcggtcgtc
      421 atcgatgcgc tggagcccaa cgccaccatg acgctgaaat ttggcgaaca gtcgactagt
      481 gacaacacgc cgttaaccac ggccgagatg ctcgatccgg tgttcgattt cgatgcgcag
      541 atggcattga cgagcggtgg tcagacagtc agcgcctctg ctcgcaaaat gctcacggat
      601 ggcaaggcaa tcccctgggt ttcgggcccc attgccacca ccgtgatcct cgccgatcat
      661 tccaaaggcc gggtatatga ccttggtttc gacacctacc gttccatacg tccgattttc
      721 gaagcgactt tctggcctcg gacccatcag gttcgcattc gcttcattgg ggagaacgcg
      781 aataccacga cgctacaaga tgttgcttac gaccttgcgt tgactaccgg ttccactgct
      841 cccaagtcgt tttacactcg tgccaatgtg ccccattatt tcggcacacg ctggacgaaa
      901 gtcgcctgga tccccacggc tcccgagagc aacgtgctca tcgatttcgg gcttccttat
      961 ctagcccaaa gccaattcat gcccaacttc gacacatccc ggacggttcc cgaggagcgg
     1021 ctcgcggccc tggcagcgag ttggtcggcg gctgacaagg agcttttcga tacgggcatg
     1081 tggacccctt acatgcccac cacgggtggg cgtgccgaga tcgctcccta cccttcctgg
     1141 acgaatcttt ggcttcggac gggggacccg cgagcacggg aagcagcgtt ggggcaagcc
     1201 gacctcgctg gttcctggcc tttgcattgg cgtgagggcg atgagcaaac caaattcgac
     1261 cgcaaaaaga cgatcccggg tctgggaatg cccatttcgg tcaacgccca tcccgacatc
     1321 tggtttccgg acaacaacgg gaactatcac ggaattttgc ctaccccgag aatttccccc
     1381 aaaactccat gggttgcgga tggtgctcac cagcccgatc cgttctcacc tcagtatatc
     1441 ctcactggtg atcatttcta tctcgaacag atccaactct gggctgctgc acaaaccgtg
     1501 gcgtacccct acgggcaata cggtcgtggc ctcaacggct acggtggaat tcaggaccag
     1561 atccgaggca atggctgggt catacgtaat cgcgtcaatg ccgctttcct ttcccccgac
     1621 ggaacgcctg aaaaaaccta cttccacgaa ctcatcgaag acgcttttgc cttgtgggaa
     1681 ggacagcgga acatccaggg cacgccactc gccaatcatc cgcaatgggc ttatgggcac
     1741 gatacgattc ctgtgacagc ttcgcctttg cacatttttg accgaggaat cgacctggca
     1801 tcgacttcat tgaacggact cgaccccaat acgacctcga gaattacggc attgtggcag
     1861 cattactact ttcttattga actcggtgtg gcgcgagacc gtggctttcc tgcagaaccg
     1921 ctgctgaaat ggcttgcacc ggtgctcaat gggcagttca ccgatccggg ctacgacccc
     1981 gttctttgtc gtatgtacat gacccccatt gccgatgtaa gcggcaatta ctttcccacg
     2041 tgggcggaca ttgccaacgc gattccacaa tccacctatg aggcacaaaa aacggcaggg
     2101 ggaagcaatt actatccact tttggcggca agcgccgcgt ccgccacgta ttgggaacct
     2161 ggaggcgagg aggcgtgggc atggctcagc acgcacgtgc gcgcgacgag tcaaacagat
     2221 tacggcaccg agtggtttag ttgggatttg ctaccccgcc ctgaggggac gaaacctctt
     2281 ccgccgtcca cgccctgaat tctgtggtta gcctcatctt tcctcctctt tttcgggggt
     2341 ttcttgggag ttattcggca ttgcggaagc tagattcaga agcaccatcg cacgacgtcc
     2401 gcgattgcgt atctcgctcc ctttgaacta cctgcctccc accgcctccg atttaccacc
     2461 gataaacgca ttgtcgccgc gttttctaga atgaccaacc aggcaaccgg tggcccatcg
     2521 tggcctgttg cacgaagatc gtcccggaga aagcacggaa ctggttatcg taaggcgttt
     2581 gccccaggag tagcgagttg caactcagga aatgggatcc cggcatgtcg atttcggctc
     2641 cgatatcgca aaccacggga ggaagtcgtc ggctcgatgc tctacgggac ccgcccctct
     2701 tctccggcgg actccgcccg aacggaaggg tccgcggtaa ttgtcgtggt ttccccacag
     2761 cgaagctgca ccgactgccc ttttgccaac cactgcaccg acataagccc tttggccccc
     2821 atttcggaac ccacccgaac gaatcgttga ccacaatccg tcaaaatcgg tgaatcggtc
     2881 gcccctagag gttttcccat cacatacacc atacccgtcg gtggtcctcg aacgacaagc
     2941 acggcttggt ctgttcccgc gctcggcatt gacggttttg aaggcgtgga aggctcaggt
     3001 acgcgatccg agtcgtcatt gcgcgaggtg ggttccgtgg gaatcgtggt tggcaagggg
     3061 ggaggaaggc cagcattcga cgaaaacgcc ccattcgcca acgcaaacag cagaatcaac
     3121 agtgtgagaa gcacggggat caccaacacg agaacccgca cgattcgacg acgtttctgc
     3181 gcggcgtgta gcaggtcgag gtcgtcatcc cctcctgttt ctgcaggtaa ataggggttg
     3241 gaatgggtaa aggcaggagc gggagtggtg gtgccggact ccgcggcggc atctccataa
     3301 ccaactgaat ttattgctgt aattcgatta tcgacaacga tttcctcggt cgttggctcc
     3361 tcc
//
LOCUS       DAOSOR010000626         3361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_28372_length_3361_cov_3.883545, whole genome shotgun sequence.
ACCESSION   DAOSOR010000626 DAOSOR010000000
VERSION     DAOSOR010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28372_length_3361_cov_3.883545"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..1185)
                     /gene="hemW"
                     /locus_tag="PK710_22705"
     CDS             complement(31..1185)
                     /gene="hemW"
                     /locus_tag="PK710_22705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOT12609.1"
                     /translation="MRTSPCSPAKKTEQALTSASQAGCYVHVPFCVSKCRYCAFFSET
                     STALMHDWLEAVISEARSLKDSFSTFDTLYLGGGTPSVLPDRLIENLLERLRNVLHFD
                     RHLEITMECNPGDLDFARASRLHTMGISRVSLGVQSFDDATLRFLGRRHDARCAQIAV
                     EQLRHARFDHIGLDLIWGIPHLPVDRTLASVESALSFDPEHLSCYSLTVESATPLHKD
                     VSSGVVRMKDDEALAEEAMRLWARLSQAGYDHYEVSNFARSPAYRSRHNTKYWYHTPV
                     LGLGPAAHSFDGSRRWANVSSVSDYVNRIRAGQRAVTFEEVLSNEQKISERVVLGLRT
                     SDGVARSIVEGRRLKELQELGLLIVTRDRVSPTERGMLVADSLAVALDPT"
     gene            complement(1148..2944)
                     /gene="lepA"
                     /locus_tag="PK710_22710"
     CDS             complement(1148..2944)
                     /gene="lepA"
                     /locus_tag="PK710_22710"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOT12610.1"
                     /translation="MNFIRNFSIVAHIDHGKSTLADRLIVGADLVSERNFREQILDNL
                     DIERERGITIKSQTVSLPYRANDGHDYILNLIDTPGHVDFTYEVSRALASCEGILLLV
                     DATQGVQAQTVANLYLAMEYDLEVIPVINKIDLPSADIDAARSQIDKELGLDGSLAVP
                     VSAKTGEGLDALREAIVERIPPPKTPQDDRLRAVIFDAAYDPFRGSVISCRVFSGSIK
                     QGDTLLLMSNGLTRRVEEVGEHRLVRTPTKSLSAGSVGYIIAGIKTIADTRVGDTITL
                     ADDPVDKPMTGFREPKPVVFSSIYPVASDDYPSLVDALQKYTLNDASLVYTKDSSAAL
                     GQGFRAGFLGLLHLEIAQERLEREFNQSLILTAPSVEYHFTLNDGSKISVDNPTYYPD
                     PTTIALAEEPYIRASILTPDRYMGAIMKLCIERRGENSELRYPGPGRVEMLFDMPLAE
                     VLFDFYDQLKSMTQGYGSFDYTLIDYRESDLVKLDILVNGEKVDALSMIVHRERSRER
                     AVHACERLKDVIPRHMFKVPIQGAIGGKIIARETIPAFRKDVTAKCYGGDISRKRKLL
                     EKQKAGKRRMKMVGNVEIPQNAYVAVLTSKKD"
     gene            complement(3060..>3361)
                     /locus_tag="PK710_22715"
     CDS             complement(3060..>3361)
                     /locus_tag="PK710_22715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOT12611.1"
                     /translation="PPSAKPAEPAKKTEKPADKPAEKPTQPARPTPPESPSAKPAEPA
                     KKTEKPADKPAEKPAEKPSQPARPTPAQPPSAKPAEPAKKTEKPAEKPAAKPAAP"
BASE COUNT          684 a          831 c         1064 g          782 t
ORIGIN      
        1 atcgtcatcg aagcatgcta gctgtcgcat tcatgtggga tccagcgcta ccgccagcga
       61 atccgcaacg agcatcccgc gttccgtggg cgacactcta tcccgagtca cgatcagcaa
      121 tcccaattct tgcagctctt tcagccgacg accctccacg atcgatcgcg ctacgccgtc
      181 ggaagtgcgc aatccaagaa cgacacgttc cgatattttt tgttcgttcg acaacacctc
      241 ttcgaaggtc acagcgcgct gtccggctcg aattcgattt acgtagtccg acacggacga
      301 gacattggcc cagcggcgcg atccatcgaa ggaatgggcg gctggtccca gtccaagaac
      361 gggcgtgtgg taccagtact tcgtgttgtg gcgcgaacga taggcaggcg agcgtgcgaa
      421 gttcgagact tcataatggt catatccggc ttgggatagt cgcgcccaca gcctcatggc
      481 ttcttccgcc aaagcctcat cgtctttcat ccggaccacg ccggaagata cgtctttgtg
      541 caggggggtg gcggattcga ccgtgagcga ataacacgaa agatgttctg gatcgaagga
      601 tagggcagat tcgacggaag ccagggttcg gtcgacaggt agatggggga taccccaaat
      661 caagtcgagt ccgatgtgat cgaaccgggc gtggcgcagt tgttcgacgg cgatttgcgc
      721 gcatcgagcg tcatgacgac gtcccaagaa tcgcaaggtg gcgtcatcga aggattgaac
      781 accgaggcta actcggctga ttcccatggt atgcaagcga gaggcgcgag cgaagtcgag
      841 gtcgcccgga ttgcattcca tggttatttc gagatgacga tcgaagtgca atacatttcg
      901 aagtctttcg aggaggtttt cgatcaaccg atcgggcagg acacttggcg tacctccgcc
      961 gagatagagc gtatcgaagg ttgagaacga gtctttcagc gatcgagcct cggagatgac
     1021 ggcctcgagc cagtcatgca tgagggctgt cgaggtctcg gaaaaaaagg cgcaatagcg
     1081 gcatttgctc acgcaaaacg ggacatggac ataacaaccg gcttgggaag ctgatgtcaa
     1141 agcctgttca gtcttttttg ctggtgagca cggcgacgta cgcattttgg ggaatctcga
     1201 cgttgccgac cattttcatg cgtcttttac cggctttttg cttctcgagg agcttacgtt
     1261 ttcgggagat atcgccaccg tagcacttgg cggtcacatc ttttcgaaat gcggggatgg
     1321 tttcgcgagc gatgattttc cctccgatgg cgccctggat gggcactttg aacatgtgtc
     1381 gagggatgac atctttgagt cgttcgcagg cgtgaaccgc acgttcgcgc gaacgttcgc
     1441 gatgaacgat catcgacagg gcatcgactt tctcgccgtt cacgaggata tcgagtttga
     1501 ccaggtcgct ttcccggtaa tcgatgagcg tatagtcgaa ggaaccatat ccttgggtca
     1561 tgctcttgag ctgatcgtag aagtcgaaca ggacttccgc gagtggcata tcgaagagca
     1621 tttctacacg gcctggtcct ggatagcgga gttccgaatt ctcgccgcgg cgttcgatac
     1681 acaatttcat gatcgcgccc atgtaccggt ccggggtaag gatacttgcg cggatataag
     1741 gttcttcggc cagagcgatc gtcgtggggt ccggatagta tgtcggatta tcgaccgata
     1801 tcttggaacc atcgttgagg gtgaagtggt attcgacact gggcgcggtc aggatcaggg
     1861 attgattgaa ttcgcgttcg agtctttctt gcgcgatttc gagatgaagc agtccgagga
     1921 atcccgcgcg aaatccttgt ccaagagccg cggaggagtc cttggtatac acgagggaag
     1981 catcgttgag cgtgtatttc tggagagcat cgaccaggct cgggtaatcg tcggaagcta
     2041 ccggatagat tgatgaaaac accacgggtt tgggctctcg aaagcccgtc atgggtttgt
     2101 ccaccggatc gtcggcaagc gtgatggtgt ccccgactcg ggtatcggca atcgttttga
     2161 taccggcgat gatatagcct acggagcctg ccgacaggga tttggtggga gtgcgcacca
     2221 gccgatgttc ccccacctcc tcgacccttc gagtcaggcc gttggacatg agcaacaacg
     2281 tatcgccttg cttgatgctt cctgaaaaaa cacggcacga gatgacggaa ccgcgaaaag
     2341 gatcgtaggc agcgtcgaaa atcacggcac gcaagcgatc gtcttgtggc gttttgggag
     2401 gcgggatgcg ttcgacaatc gcttcgcgga gcgcatcgag cccttcaccg gttttcgcgg
     2461 atacgggaac ggcgaggcta ccatcgagcc ccaattcctt atcgatctgg gagcgtgcgg
     2521 catcgatatc ggcggagggg aggtcgattt tgttgatgac ggggatgact tcgaggtcat
     2581 attccatcgc gagataaagg ttagcgacgg tttgcgcttg cacgccctgg gttgcatcga
     2641 cgagcagcag gataccttcg caggaggcca gagcgcggga tacttcgtag gtgaagtcga
     2701 cgtgtccagg ggtatcgatc aagttgagga tgtagtcgtg tccatcgttg gcgcggtagg
     2761 gcaacgagac cgtttggctt ttgatcgtaa tgccgcgttc gcgctcgata tcgaggttat
     2821 cgagtatctg ttcgcgaaag tttcgttcgg agacgaggtc tgcgccgacg atgagccggt
     2881 cggccagggt tgatttgccg tggtcgatat gcgcaacgat gctgaagttt cgaatgaagt
     2941 tcatgggagt cggcaacgaa ggagggagag cctgccaggg ttcggccgtg ggtcaaggcc
     3001 aatcaacagc gagggaaagg aaggagaaca ggagtaggca gaggctattg atggtgtttt
     3061 tatggcgcgg cgggctttgc cgcgggcttt tccgctggtt tttctgtctt tttcgcgggt
     3121 tcggcaggct tggccgaagg cggctgggcc ggagtcgggc gcgcgggttg cgagggcttt
     3181 tccgcgggct tttccgcggg tttatccgct ggtttttctg tctttttcgc gggctcggca
     3241 ggcttggccg aaggcgattc cggcggagtc gggcgcgcgg gttgcgtggg cttttccgcg
     3301 ggtttatccg ctggtttttc tgtctttttc gcgggttcgg caggcttggc cgaaggcggc
     3361 t
//
LOCUS       DAOSOR010000627         3357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_28421_length_3357_cov_4.455179, whole genome shotgun sequence.
ACCESSION   DAOSOR010000627 DAOSOR010000000
VERSION     DAOSOR010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28421_length_3357_cov_4.455179"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PK710_22720"
     CDS             complement(<1..211)
                     /locus_tag="PK710_22720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12612.1"
                     /translation="MQLQQIAYALVLMLAFGAYWWWRSRGRGGRQTEVYLGLSPGEEL
                     ETYASGRHHLDFGVADVGAAALGMER"
     gene            602..1969
                     /locus_tag="PK710_22725"
     CDS             602..1969
                     /locus_tag="PK710_22725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12613.1"
                     /translation="MAFTAFQQRCPQILAACPELQAYQEWLKTQRTPSSRDYLFSQTR
                     VRFEPRKQDVVSLLPGLSVAHKNKRTVLISARPFHEIVLDGVTVQQAERILRAFDGQR
                     TLLEARWDSGVSPGCFASFLRASFGWVVFAPAAIAQLENDLSGTEITRFPTVPYGIER
                     AYWENMIDVRAYARLHLEALSSTADVLRLLRELHVLALLGRHLNSFYKPASPIADQTV
                     APGALYLDMPRLLERGERTIFLDGPRVNVSLLGGQAYHDALYRSLDDAEALAPSRIFS
                     SGGVDWGRVVTARSEKDDSFGPWFCPPRPIVDRHWDKLAGELMGAVKAASNRNMQAMT
                     DGLASFHQTFVRLHPFHCANQSIAMNLVNAVLTMAQGFGIPHLILDLLALRLSETAYR
                     KLLARAVRAYGVGGMDAPSRLSTLMARSAAMNAVVEAMAGGSSQEQHAGRLAADDAGW
                     ALLSD"
     gene            complement(2298..>3357)
                     /locus_tag="PK710_22730"
     CDS             complement(2298..>3357)
                     /locus_tag="PK710_22730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT12614.1"
                     /translation="DPLNNPLVDAPNIKYQTAGALRVFFRGLVQVVFPRQLSGDYSAP
                     QEPVPQHLFEVENVLGAAAFVLIPIIAIALAIRAWWIERRVFRLLCGSTGRSRKELDP
                     NLARLRLPMLGMLGAVGLMWVVVAYFPHSNIPVLLPTVRAERFWYFPVIGSSLALAAG
                     FTWLYESTRQRWHGALAVGICAAFLTFQAARARTHAFHYADDLAFWDATRKAVPRSAK
                     AHLNYSVMWGARGRLDIRLHANKEALALAPSWPMAYIYLGDTLCRLHRPEEAWPYYQQ
                     GFEKAPGDPNLIALSLQCLWDEVALEDHRDELLTMSKKHPGSWLEYLVNDIFNHGEEN
                     NGVDPKYRPRGYNEGPRE"
BASE COUNT          704 a          935 c          977 g          741 t
ORIGIN      
        1 cgcgctccat acctagcgcg gccgctccga catcggcgac accgaagtcg agatgatggc
       61 gaccgcttgc gtacgtctcc agctcctctc ccggagacag gccgagatac acctccgtct
      121 ggcgaccgcc tcgaccgcgg ctcctccacc accagtacgc gccgaacgcc agcatgagaa
      181 caagggcata tgcgatctgt tgcagttgca tgtgtgaatt cacaaatcat tacctcaccg
      241 tcacgacgac gtctacgact gcttgatcac attcgcgacg gtccacgccg tggtgctgtt
      301 gggtcgaaca ggcgacggca gagcggctac aaaccgggta cagaacagta cctgttttcc
      361 ccgtggcggg accgcagcat cattgtaact cattgaattt gcagaacttt actccttgag
      421 cctgcgcagg gggtcaacga gtcggtgatt cgaatccact cgccccgact gaaagcccgc
      481 tgaagattca gcagattttg gttttgtggt cgcttgctcc tcacgaagcc acgaaaacaa
      541 tattacggat acgcctccgc gccggctacc tcccctcggc tcgccagagt agcatcccga
      601 catggctttt accgcgttcc aacaacgatg cccccaaatt ctcgcggcgt gccccgaact
      661 acaagcctac caggaatggt tgaagaccca acgcacccca tcgtcacgcg attatctgtt
      721 cagccagacg cgcgttcgtt tcgagcctcg caaacaagac gtcgtttctc ttcttccggg
      781 cttgtcggtg gctcacaaaa acaaacgcac cgtattgatc agcgctcgtc cgtttcacga
      841 aatcgtgttg gatggtgtga cggttcagca agcggagcgg atcctgcgcg ccttcgatgg
      901 gcagcgaacc ttgctcgaag cacggtggga ttcgggcgta tcccccggct gttttgccag
      961 ctttctccgc gcctcgttcg ggtgggtggt cttcgcgccg gccgcgatcg cccagctcga
     1021 aaacgatctt tccggcaccg aaatcacacg atttcccact gtcccttacg ggatagaacg
     1081 agcctattgg gagaatatga tcgacgttcg tgcgtatgcg cgtttgcatc tcgaagcgtt
     1141 atcgagcacc gcggatgtcc ttcgtctgtt gcgggagttg catgtcttgg cgttgttggg
     1201 gcggcacctg aacagcttct acaaacctgc gagccccatc gctgatcaga ccgtggcgcc
     1261 cggcgcgctg tacctggata tgccgcgtct tctcgaacgc ggggaaagaa ccatctttct
     1321 cgatggtccg agggtcaacg tatcgctgct cggtgggcag gcctatcacg acgctttgta
     1381 ccgttcgttg gatgatgccg aagctctggc tccaagccgg atattttcat cgggtggggt
     1441 tgattggggg cgagttgtga cggctcggtc ggaaaaagat gattctttcg gtccgtggtt
     1501 ttgtcctccg cgacccatcg tggaccggca ttgggataag cttgcggggg agctgatggg
     1561 ggcggtcaaa gccgcgagca atcggaacat gcaagcgatg accgatggct tggcttcctt
     1621 tcatcagacc ttcgttcggc ttcatccttt tcattgcgcc aaccaatcca tcgcgatgaa
     1681 cctcgtcaac gcggtgttga caatggcgca aggcttcgga attcctcatc ttatcttgga
     1741 tctcctggcg ttgcgtctgt cggaaacggc atatcggaag ctattggcaa gggcggtacg
     1801 agcctacggc gtgggaggaa tggatgcccc gagccggctc agcacgctca tggcgcgcag
     1861 tgccgccatg aatgccgtgg tcgaggcgat ggccggtggc tcttcgcaag aacaacacgc
     1921 gggccgattg gccgcggacg atgcggggtg ggctttgttg tcggattagg gtgcgatttc
     1981 caggcaaggg gacagccagc ttggggcggc ccccaattcc tatttctagt tcctagtttt
     2041 agttatgatt ttaattctaa ttttagctac ttgttttggt ttgtttttgc gaactgtgtc
     2101 ccaacctggc cctctccccg ccatagctaa ccgaaaaggg aaggaccggg gacagatgca
     2161 cccaaatcgg gaaaggatac cttttatcac tgcgaataat ggaattatgt gaaggattta
     2221 tattgccaag cgaaacggat ctgaaaacca ggaactagga accaggaatg cgaattagat
     2281 attaatatta caccctacta ttcccgtggt ccttcgttgt acccacgagg acggtacttc
     2341 ggatctaccc cgttgttttc ttccccgtgg ttgaaaatat cgttgacgag gtactcgagc
     2401 cacgaacccg ggtgtttttt ggacatcgtc agaagttcgt cgcggtggtc ctccaacgcc
     2461 acttcatccc agaggcactg cagggatagg gcgatcaggt ttgggtcccc cggcgctttt
     2521 tcgaaacctt gttggtaata aggccacgct tcttcgggcc ggtgcagacg gcataaggta
     2581 tcgcccaggt agatataggc catgggccaa gagggagcga gcgcgagtgc ttccttgttg
     2641 gcatgcaaac ggatgtcgag cctgcccctt gcgccccaca tgaccgaata attcaggtgt
     2701 gccttggcgc ttctggggac agcctttcgg gtagcgtccc agaaggcgag atcgtcggcg
     2761 tagtgaaatg cgtgggttcg tgctctggca gcttgaaagg tcaaaaaagc ggcgcaaatg
     2821 cccacggcaa gggcgccatg ccaccgttgg cgtgtggatt cgtacagcca cgtgaacccc
     2881 gcggcgagag ccaggctgga gccaatgacg ggaaaatacc agaagcgctc cgcgcgaacc
     2941 gttggcaaaa gcacggggat attcgaatgc ggaaaatagg ctaccaccac ccacatcaag
     3001 ccaaccgctc cgagcatgcc gagcatcggc aggcggagtc gggcgagatt ggggtcgagt
     3061 tctttgcgcg agcgacccgt tgaaccgcaa agaagtcgaa agactcggcg ttctatccac
     3121 caggcgcgaa tggctagcgc gatggcgatg atgggtatga ggacgaaagc cgcggctccg
     3181 agcacatttt ctacttcgaa aaggtgttgg ggtacgggct cctggggcgc cgaataatcg
     3241 ccggagagtt gccggggaaa tacgacctgc accaatcctc gaaagaatac gcgcaacgca
     3301 ccggcggtct ggtacttgat gttgggagca tcgaccaacg ggttgttcaa gggatct
//
LOCUS       DAOSOR010000628         3351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_28491_length_3351_cov_2.951760, whole genome shotgun sequence.
ACCESSION   DAOSOR010000628 DAOSOR010000000
VERSION     DAOSOR010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28491_length_3351_cov_2.951760"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1583
                     /locus_tag="PK710_22735"
     CDS             <1..1583
                     /locus_tag="PK710_22735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12615.1"
                     /translation="AVLRETDMAATLSELAFIDKCSPDAEYLGSAAHRDNAASAHCIA
                     ITSHLGVNGSCSAAPPTDGKAMGAIFEDKGSGTDDMSTRLEGATATVKGTSMTMTVSG
                     PDAFYSFDLKAGTYDIEASMDGYDSNTVSCVVTAGQTQWCSIGLKKSEPAKPDGGLDQ
                     PDARPDVFTSPDAAEQDVEREAGQEDAEQEDARRPPGPNYWSSNDDSGCSCRTTQPRG
                     GLGFGVGTLMALGLFAARRRRLLGGLMGLSALALLGCDQDASHHGTITKATAPLATQP
                     TAQPTPRLASQRKILNSQYILPVLSPDGAWLAATHPGLNQLWIASTSDGALKNISQAP
                     RSGYMPSWRADSQAIAFRDGNADPSGNIWSFSDLAAQRAASFLSSNTVSVRQTEDDRI
                     VLRVGGREHTIDEGKDKYFSPSVSQDGSWVVYTGITLGIHLYHVPRGETVQLGQGTHP
                     SLSADGTWLAYEKTDDDGHSLISGDLYLCDLRDTAYPVFNLTRTPREIERTPSLSADG
                     RLIAYVQSDGVYLATIER"
     gene            1796..3211
                     /gene="miaB"
                     /locus_tag="PK710_22740"
     CDS             1796..3211
                     /gene="miaB"
                     /locus_tag="PK710_22740"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOT12616.1"
                     /translation="MKRFYIRTLGCQMNMHDSDRVHELLCDAGMSPVDAPELADLVVV
                     NTCSVREKAESKLDSELGKLRKLKTSRKGAMVLVVAGCVAKQRGQAMLDRMPFVDVVL
                     GPDNLMSLPRLATAAAMGAPPTVATDWDVDQPTFLRSRASNEGLRPSAFVSISKGCNE
                     RCSYCIVPSTRGPERHRPASEIVDEIQELVSFGALEVILLGQTVNGYQDPTGELGARP
                     SHALSDFGALLQRIARDVPRLARLRYTSPHPRYFDPSVIEAHRQLEVLCAHVHMPVQS
                     GSDRMLKRMIRRHDRAEYLEAIRQLRRARPGLTVSTDLIVGFPTETDDDFEQTLSLMQ
                     EVPFLGVYAFKYSPRPGTAALRLGDDVPDSLKAERLARVFDLSERCMQQHLEGLVGTS
                     QRVLIEEPNARGDGGVSGHTDRNEIVHVEPSGGLRVGDGIVPLRIVRAYKHSLVGELD
                     RTDATVKNNLRYRLAVVSGEP"
BASE COUNT          671 a          951 c          986 g          743 t
ORIGIN      
        1 ttgccgtgct gcgagagacg gatatggcgg cgaccctgtc agaactggct tttatcgata
       61 agtgcagtcc agatgcggaa tacctgggaa gcgccgccca tcgcgacaac gcggcgagcg
      121 ctcattgcat cgcgatcacc agccacctgg gcgtgaacgg ctcttgttcg gcagctcctc
      181 ctaccgacgg caaggccatg ggagccattt ttgaagataa aggctcggga accgacgaca
      241 tgtccacgcg tctcgaaggc gccaccgcca cggtgaaagg cacgagcatg accatgaccg
      301 tcagtggacc ggatgctttt tattccttcg atttgaaagc cggtacttat gatatcgagg
      361 cttcgatgga tggatacgat tcgaatacgg tgtcttgtgt ggttaccgcc ggacagaccc
      421 aatggtgctc gatagggctt aagaaatccg agcccgccaa accggatggg gggttggatc
      481 agccggatgc gcgaccagac gtatttacca gtcctgacgc cgctgaacag gatgttgaac
      541 gagaagctgg tcaagaggat gcggaacagg aagatgctcg ccgtccgcct ggtcccaact
      601 attggagttc caacgatgat tccggctgtt cttgcagaac gacccagcct cgcggcggcc
      661 tcggcttcgg tgtggggacc ctcatggctc tgggcctttt cgcggctcgt cgacggcgcc
      721 tgctcggcgg cctgatgggg ttgtccgcac tcgcactgct tggctgtgat caggacgcgt
      781 cccatcacgg cacgatcaca aaagcgacag ctcccttggc cacgcagcca accgcccagc
      841 cgaccccgag gctcgcgtct cagcgtaaaa tattgaattc acaatatatt ttacctgttc
      901 tgagccccga tggcgcgtgg ctggccgcca cccatccagg gctgaaccag ttgtggatcg
      961 cttctacttc cgacggtgcc ttgaaaaaca tttcccaggc tccccgcagt ggatatatgc
     1021 cgtcgtggcg cgcggatagc caggccattg cgtttcgcga cggaaatgcc gatccgtctg
     1081 gaaatatttg gtctttttcg gatctggccg cgcaaagggc cgcctcattc ctgtcctcga
     1141 ataccgtaag cgtgcggcaa accgaagacg accgaatcgt gctgcgggtc gggggccgtg
     1201 agcatacgat cgacgaaggc aaggataagt acttctcacc atcggtgtcg caggacggat
     1261 cctgggttgt ttatacggga atcaccctgg ggattcatct ctatcacgtg ccgcgggggg
     1321 aaaccgtgca actcggacag ggcacgcatc catcgctgtc cgcggatgga acgtggttgg
     1381 cctacgaaaa gaccgatgac gacggacatt cgttgatttc cggggacctg tatctttgcg
     1441 acttgcgcga caccgcgtat cccgtattca atcttacccg cacaccgcga gaaatcgaac
     1501 gcacgccttc tttgtccgcg gatggtcgct tgatcgcgta tgtccaatcc gatggggtgt
     1561 atctggctac gatcgagcgc tgacactgtc ctacgcccat cctcttctcg atgtcgagaa
     1621 ggcgcccctg tattctgttt tctcgccttt cctgaatgtt tcttccgtaa tgtttaatgg
     1681 aagaagagtc ccctctgttt ttcatcaacc tctaggagct gatggccgtg cttggggcgc
     1741 gacggtgatt gccagggtct gggctctacg ctaagagaag cgttgtccgg tcgtgatgaa
     1801 acggttttat attcgaacgc ttggttgtca gatgaacatg cacgattccg atcgcgtgca
     1861 tgaattgttg tgcgatgcag gtatgtcgcc cgtggatgcc ccggagttgg cggacttggt
     1921 ggtggtcaat acctgcagtg ttcgcgaaaa agcggaatcc aagctggata gcgagctggg
     1981 gaaactgcgc aagctgaaaa cctcgcgaaa aggtgcaatg gtgctggtgg tcgccggttg
     2041 tgtcgcaaaa caacgaggcc aggccatgct cgaccgtatg cctttcgtgg acgtggtgct
     2101 cgggccggat aacttgatgt ccttgcctag gcttgcgacc gcggcggcca tgggcgctcc
     2161 ccccaccgtg gcgacggatt gggacgtgga ccaacctacc ttccttcgct ctcgcgcctc
     2221 caacgagggc cttcggccgt ccgctttcgt gtccatctcc aaagggtgta acgaaagatg
     2281 ttcgtactgt atcgtgccgt cgacgagagg accggaaaga catcgcccgg catctgaaat
     2341 tgtagatgaa attcaagaac ttgtgtcttt tggggcgctg gaagtgatct tgctcggtca
     2401 gacggtcaat gggtatcaag acccgaccgg tgaactcgga gcgcgtccca gccacgctct
     2461 atcggacttt ggggcccttc tgcaacgcat cgcccgtgat gtgccgcggt tggctcgtct
     2521 tcgttatacc agcccccatc cgcgatactt cgacccatcg gtcatcgaag ctcatcggca
     2581 gctcgaagtg ctttgcgccc acgtacatat gcccgtccaa tccggcagcg accgtatgct
     2641 caaacgcatg attcgccgtc acgatcgcgc cgaatacctc gaggcgatcc ggcaattgag
     2701 gcgcgcgcgc cctgggctca cggtatctac cgacctgatc gtggggtttc ccaccgaaac
     2761 cgacgatgat ttcgagcaaa ccctgtcgct catgcaggaa gtaccgtttc tcggggtgta
     2821 tgcattcaag tattcccctc gccccggaac cgcggccctt cgtcttggcg atgatgtgcc
     2881 cgatagcctg aaagccgagc gattggcgcg agttttcgac ctgtccgagc gatgcatgca
     2941 acaacacctc gaaggtctcg tcggcacctc gcagcgcgtg cttatcgaag aacccaatgc
     3001 aaggggcgac ggtggcgtga gtggtcacac cgaccgcaat gaaatcgtac acgtggagcc
     3061 ttccggcgga ttgcgcgtgg gcgatgggat cgtgccgttg cggattgtgc gcgcgtacaa
     3121 gcattcgctc gtcggtgaac tcgatcgaac ggatgccacg gtgaaaaata atcttcgtta
     3181 tcggcttgcc gtcgtcagcg gtgagccatg agcgataacg ggaggtaacc atgcccctga
     3241 ttcttccgtt tcaacagcat attcccaaaa ttcatcccgg cgtctgggtg gcgccgcatg
     3301 cgacgatcat cggtgatgtg gagattgagg aaggcgcttc gatatggttt g
//
LOCUS       DAOSOR010000629         3349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_28515_length_3349_cov_3.937766, whole genome shotgun sequence.
ACCESSION   DAOSOR010000629 DAOSOR010000000
VERSION     DAOSOR010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28515_length_3349_cov_3.937766"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="PK710_22745"
     CDS             complement(<1..1144)
                     /locus_tag="PK710_22745"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOT12617.1"
                     /translation="MLSWLLRYRLLVLIASVAIATGGVFAWTRLPIDAFPDVTNTQVM
                     ILSEAPGYAAVDVEQRVTYPIEQAMRGLPRVVQVRSLSKPGLSQVVVVFEDDAETYWT
                     RQLVFERLATVREHLPPGVEPELGPISTGLGEIYQYTLEGEGKTAMELRTIQDWIVAP
                     LLKPIAGVNEVNSFGGEVKQIQVLVSPEKLLAFGLTVQDVVEAVEASNANAGGGIVVR
                     DWEQMYMRGVGLLQDIPDLESTVVDVKDGTPVYLRDVAEVTIGAEVRQGAVTRDGKGE
                     VVAGMIVMLKGENSKEVVSRVKDTVDRIGGTLPEGVKINVFYDRTSLIEACIATVRNA
                     LLEGGILVIIVLFLFLAELRTSFIVLVSLPLTFLVSFIVMGWLGISS"
     gene            complement(1156..2478)
                     /locus_tag="PK710_22750"
     CDS             complement(1156..2478)
                     /locus_tag="PK710_22750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT12618.1"
                     /translation="MEKGRRSTSLVKWGSPFAVVLLWWGTSACERTSHAQDSKPREVV
                     SVSEAKADDHDHDHDHGSESDLDKSAESLFNQDCEHGIKAFSCDECRYEVGVVRVAEP
                     LMRQGLFELVHPGERAVAAPLELTAEVRFDDRLVAHVRPVAQGIIRKVHVVIGDEVKQ
                     GAPLLEMDSVAVGDAYGELLEARARLVLAKQSYERAESLKKSGLASEKDYLEVKTDYE
                     AARIRVDAAQGRVARLGGTGAGTIVLRAPMTGKVLELHAVPGEVASSDESIATVGNHD
                     AVWVWADLFERDLAKVMDAQNGGKLEASVFVEAYANEAFSGVVDLVSPSMDRKSRTVK
                     VRVAVDNPKGRLLAGMFAKVQVFLPGGERALAVPREAVLEDEGRSFVFVHHHGEYFVR
                     RPVRTGRAWGGWVEVVSGVKAEQQVVGRGAFLMKSDVLRSKMGAGCAD"
     gene            complement(2999..>3349)
                     /locus_tag="PK710_22755"
     CDS             complement(2999..>3349)
                     /locus_tag="PK710_22755"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOT12619.1"
                     /translation="VEARLGSAEQVANDVPTTVPVVVATHEHALDLAAIVWAIERGHT
                     YVGGIGSKTKSVKTRRALEERGVPKAVVDSLRMPVGLKLGGRTPEEIALAIAAELVAW
                     RAGTLDKAATKQDE"
BASE COUNT          764 a         1183 c          761 g          641 t
ORIGIN      
        1 tggacgatat gcccagccaa cccatgacga tgaaactcac caaaaaagtg aggggcaggg
       61 aaaccagcac gatgaacgaa gttcgaagct ccgcaagaaa caaaaacaaa acgatgatga
      121 cgagaatgcc cccttccaat aacgcgtttc gcaccgtcgc gatgcacgct tcgatcagcg
      181 atgttcgatc ataaaataca ttgattttca ccccctccgg cagcgtgccg ccgattcgat
      241 cgaccgtatc cttcacccgg ctcacaacct ctttcgagtt ctcccccttg agcatgacga
      301 tcatgcccgc taccacttcc cccttaccat cgcgcgttac cgctccctga cgaacctccg
      361 cgccaatcgt cacctccgca acatcccgaa gataaacagg cgtcccgtct ttcacatcca
      421 ccaccgtgct ctcgagatcg ggaatgtctt gcagaagacc cacgccacgc atgtacatct
      481 gctcccaatc gcgaacgacg atgcctcctc ccgcattcgc attgcttgct tcgacggcct
      541 cgaccacgtc ttggacggtg agcccgaaag ccagtagttt ttctggcgaa accaggactt
      601 ggatctgctt gacttctccg ccaaaactat tgacttcgtt gacaccggcg atgggtttga
      661 gcaaaggcgc gacgatccag tcctggatgg tccgtagctc catcgcggtt ttcccctccc
      721 cctccaacgt atattgatag atttcaccta gccctgtgct gatcggccca agttccggct
      781 ctacccccgg tgggagatgc tctcgcacgg tcgccaacct ctcgaacacg agttgtcgag
      841 tccaatacgt ctccgcatca tcctcgaata ccaccacgac ctgggataat cccggcttcg
      901 acaaggagcg cacttgcacc acacgcggca acccgcgcat cgcttgctcg atgggatagg
      961 tgacacgctg ctccacatcc accgccgcgt accccggtgc ctcgctcaaa atcatgactt
     1021 gggtgttcgt cacgtcagga aacgcatcga tgggaaggcg ggtccaagca aacacccccc
     1081 ctgtcgcgat ggccacggag gcaatcagga caagaagtcg atatcgcaga agccaggaaa
     1141 gcatattcga cccgatcaat ccgcgcagcc agcgcccatt tttgagcgta acacatcgga
     1201 tttcatgaga aatgcgcccc gccccaccac ttgctgctcc gccttcacgc ccgaaacgac
     1261 ctccacccac cccccccagg ctcgtcccgt tcgaaccgga cgacgcacga aatactcacc
     1321 atgatgatgc acgaaaacga acgaacgccc ctcgtcttcg agaaccgcct cgcgaggaac
     1381 cgcgagcgcc cgttccccgc ccggcaaaaa cacctgcact ttcgcgaaca tccccgccag
     1441 aagacgaccc ttcggattgt ctaccgcaac acgcaccttc accgtacgcg atttccggtc
     1501 catcgaagga ctgacgagat cgaccacacc cgaaaaggct tcgttggcat aagcttctac
     1561 gaataccgag gcttcgagtt tgcccccgtt ctgagcgtcc atgactttcg ccaaatcccg
     1621 ctcgaaaaga tccgcccata cccaaaccgc atcatggttg cccaccgtgg cgatgctctc
     1681 atccgaagag gccacttcgc ccggaaccgc gtgcagctcg agcactttcc ccgtcatggg
     1741 agcacgcaat acgatggtgc ccgcacccgt tcccccaagc ctggcaacac gcccctgcgc
     1801 agcatcaacg cgtattctgg ctgcttcgta gtccgtcttg acctcgagat agtccttttc
     1861 cgacgccaaa ccgctttttt tcaacgattc cgcccgctca tacgactgct tcgccagcac
     1921 cagccgcgca cgcgcttcca gcaactcacc gtaagcgtcg cctaccgcca cgctatccat
     1981 ctccagcaaa ggcgcgcctt gcttcacctc atcgccaatc acaacatgca ctttgcgaat
     2041 gatcccctgc gccaccggac gcacatgcgc caccagacga tcgtcaaatc gtacctccgc
     2101 cgtcaattca aggggagcag ccacggcgcg ctcgccagga tgaacgagtt cgaacaaccc
     2161 ctgacgcatc aacggctcgg ctacgcgaac gacccctacc tcgtagcgac actcgtcaca
     2221 cgaaaacgct ttgattccat gctcgcaatc ctgattgaac aaggattccg cggacttatc
     2281 caagtcgctc tccgaaccgt ggtcatgatc gtgatcgtga tcgtccgctt tcgcttccga
     2341 tacggaaaca acttcccgag gcttggaatc ttgcgcgtga ctcgttcgct cgcacgcact
     2401 tgtcccccac cacagcaaca caaccgcgaa aggactaccc cactttacca aagacgtcga
     2461 acgcctgcct ttttccatag cacacctgcc atcccgcttg gattttcctg tgacactcag
     2521 cccacgcgac gaatgtcacc ctcgaggggg atggaacacg cgacctttca ccccttccgc
     2581 ctcggggtcg aacaggtgtg ccatgaaaag gagatttacc gaaatagggc caaacacaag
     2641 gaaatcggtg gcatcccgtg tggcgaaaag acgcccgtga cagcacgtac acaggcaacc
     2701 gtcgccacaa tcatgcccat ccgaacatgg atcgggacat gatgagccat cggcgtggta
     2761 cgccaccgtg tcgtcgttgt ggtcatccac cgccaaggtt accaaccaag gtcccaggac
     2821 gcccaaaacc agcgcgagca ccgtcaacca cgaaacgaac cgccagccgc gtggcgcggt
     2881 gcaaattggc tgtttcgtgc gacggaccct catctatccc aaaaaccttt tccgtaaacc
     2941 ataggatacc aaagccgcgc gtcaagtcgc cagcattcgg cttcgcgttg gcaactcctc
     3001 attcatcctg cttcgtagca gctttatcta aggttcccgc cctccaggct accagttcgg
     3061 ccgcaatggc caaggcaatc tcctccggcg tacgcccgcc aagcttcaac cccacaggca
     3121 tacgaagact atccactacc gctttcggca caccccgctc ctccaacgct cgccgggttt
     3181 tcacggactt cgtcttgcta cctatgccac ccacatacgt gtggccgcgc tcgatagccc
     3241 atacgatggc cgcaagatcg agcgcatgct catgcgtggc caccaccacc ggaacggtcg
     3301 ttggcacgtc attggctacc tgctccgcgg aacccaatct cgcttcgac
//
LOCUS       DAOSOR010000630         3317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_28848_length_3317_cov_4.354077, whole genome shotgun sequence.
ACCESSION   DAOSOR010000630 DAOSOR010000000
VERSION     DAOSOR010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28848_length_3317_cov_4.354077"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(352..1128)
                     /gene="pgeF"
                     /locus_tag="PK710_22760"
     CDS             complement(352..1128)
                     /gene="pgeF"
                     /locus_tag="PK710_22760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOT12620.1"
                     /translation="MHSPLALRSPRLSAAGFRHAFFTRLGGFSLPPFHSLNFASHVGD
                     DPASVARNLSIAAEDLNVDAQRILFLKQVHGIVTHLAAPPLSLDETEKWQGDALIARE
                     PSIACGVRIADCAPILMADPVSGFVAAIHSGWRGTEQNITGHVIDTLRDHGCHAHHLI
                     AAVGPHIERCCFETGHDVAARLSRASPVHDVLHLGPRGRPHVDLRRILHAQLLQAGVR
                     PNQIDHVRGCTVCDSERFFSYRRDGSRSGRLLAAIVPRKT"
     gene            1469..2539
                     /locus_tag="PK710_22765"
     CDS             1469..2539
                     /locus_tag="PK710_22765"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT12621.1"
                     /translation="MAGPDPFIGRDILDSQFRILQKIGSGGMGSVYKASQPSMNRMVA
                     VKILHPKLANRRDLVSRFRREASAMSHLSHPNTVKVFNYGELEDGSLYIVMEYLEGKN
                     LHQTVRKGGPLPYERALPILIQVCGALDEAHRKGIVHRDLKPENIFLCTDPLGQQDYA
                     KVLDFGLAKVTEREMNPGSIILTQEGMVFGTPEFMSPEQAQGKALDPRSDIYSLAVIL
                     YEALTGKLPFDAKTPMEFIQHHVATKPIDLNARVAGLSFPPALAEVLAKAIAKKPDRR
                     FQTAAEFAQALQEIVPDAATERGTIPTASPALRASITEPTPLPAQASPKVSTVPLLIG
                     VAVGSLVLGILITIVLMKFVLS"
     gene            complement(2652..>3317)
                     /locus_tag="PK710_22770"
     CDS             complement(2652..>3317)
                     /locus_tag="PK710_22770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12622.1"
                     /translation="VDVLLSQARKPETLTAYLATALARIHIEETGGFDESWPENEWRS
                     TCELAAITLGFGVLLANASYMFSKGCGGVHVDRATALQVTEIALGLAIFVAHHEITSS
                     FTNTLDPTQRAAMSEAKLWVDSNQKLIRRLRRDPAEVARDEMLEVQEAKPWLARVLGL
                     GGKKKRNLEVFDEDGFSQFEAEMKRRKTQSADSKKTDGSIDDLRALVDESLEEVRASR
                     EQV"
BASE COUNT          718 a          933 c          961 g          705 t
ORIGIN      
        1 gaaccgccgt cggcttcggc gcctccgcta ccgcccttgg cacctgaacc gcccgtgggc
       61 gggtgaagcg ctccggttcc cccattcccc tcggagtctt cgccctccgc gcatcccatc
      121 accgatagcc atgccaaaca agggagccaa agccagccat acgcgcgcat atcgaacctc
      181 ccacccaagc gaggtgtttg cttgtattca caacgtgccg cagaaatgag gcacgctccg
      241 atacccccta agcctaccgt tcttcgcccg gttggccagc acattttttc tccatccacg
      301 atgggcggag tttgttcgcg ggcctccccc cccgacccga caaccatgga ctcaggtttt
      361 tcgaggtacg atcgccgcta gcaaccgccc gcttcggctc ccatcacggc gatacgaaaa
      421 aaagcgctcc gaatcacaga ccgtgcaacc gcgcacgtgg tcgatttgat tcggacgcac
      481 gccggcttgg agcaattggg cgtgcaggat gcgtcgcaaa tccacgtggg gccgtccgcg
      541 agggcccagg tgaagcacat cgtgtacggg gctggcgcga gagagtcggg cggcgacatc
      601 atgtccggtc tcgaaacagc agcgttcgat atgaggtcct acggctgcga tgagatgatg
      661 ggcgtggcaa ccatgatcgc ggagcgtatc gatgacatgg cccgtgatat tttgttcggt
      721 tccgcgccag ccggaatgga tggctgccac gaatccggag acagggtcgg ccatcaggat
      781 gggagcgcaa tcggcaatac gcacgccaca ggcgatggac ggctcgcgag cgatgagagc
      841 atcgccctgc catttttctg tttcgtcgag ggataggggg ggcgccgcaa gatgggtcac
      901 gatgccatga acttgtttca aaaacaggat ccgctgggca tcgacgttca ggtcttcggc
      961 agcgatggaa aggttgcgcg cgaccgaggc ggggtcgtca ccgacatggg aagcaaaatt
     1021 gagcgagtga aatggcggga ggctgaaacc cccgaggcgt gtaaaaaagg cgtgtcgaaa
     1081 accagcggcc gaaagacgag gggagcggag cgccaagggt gaatgcacgg caatcagggt
     1141 atccgcgacc tgtcgccaag gggtgaacaa tcgtcgcagg cttgggatgg tagcttggga
     1201 tcgcggcctg acaaagcagg tcctctcggc gttgccttcc gattccccca tggctcacaa
     1261 ttataaaatt aatgtataaa tacaattggt tacaatagtg ccacggatcg tgggtaggct
     1321 ccatcagccc gcttttccca tgtcataccc ccaagacttc gacgtcatga ttcgttggtc
     1381 tgccgcccca cgggacgaat ggacaattgg gtggctttga agacgacgca tcgcgcttgc
     1441 cgcgcaaccg tggtattcat cgggagccat ggcagggcct gatcccttta tcggtcgtga
     1501 tatcctcgac agtcaatttc gaatcctcca gaagatcggg tcgggaggta tggggtcggt
     1561 ttataaagcg tcccaacctt cgatgaaccg aatggtggcg gtgaagatcc ttcatccgaa
     1621 gctggcgaat cgtcgggatt tggtttcgcg tttccgtagg gaagcgagtg cgatgagtca
     1681 cttgagccat cccaatacgg tgaaggtatt caattatggg gaactcgagg acggttcttt
     1741 atacatcgtg atggagtatc tcgagggaaa gaatttgcat cagacggtgc gcaagggagg
     1801 gccgttgcct tatgagcgtg cgctgccgat attgatccag gtctgtgggg ccttggacga
     1861 agctcatcgc aaagggatcg tccatcgcga tctgaagcct gaaaacatat tcctttgcac
     1921 ggatcctttg ggtcagcagg attatgcaaa ggttttggat tttggtttgg cgaaggtgac
     1981 cgaacgggag atgaacccag gttcgatcat tttgacgcag gagggcatgg tattcggtac
     2041 gcccgagttc atgtcgccgg agcaagcgca agggaaggct ctcgatccgc gcagtgatat
     2101 ttattcgctt gcggtgattc tatacgaagc gttgacaggc aagcttccgt tcgatgccaa
     2161 gacgccgatg gagttcattc agcaccatgt ggccaccaaa ccgatcgatt tgaatgcacg
     2221 ggtagcgggt ctttcctttc ctcccgcgct tgcggaagta ttggcgaagg ccattgccaa
     2281 aaagcccgac cgtcgatttc aaacggccgc cgaattcgcg caagcgctgc aagaaatcgt
     2341 gcctgacgca gccacggaac gcggcacgat cccaaccgcg tcgcccgctc ttagagcatc
     2401 gatcaccgag ccaacgccgc tgcccgcgca agcctccccg aaagtgtcta ccgttccgct
     2461 gctcatcggt gtggcggtgg gaagcctcgt tctcggcatt ctcatcacca tcgtgttgat
     2521 gaagttcgtc ctgtcctgaa ttcgttgacc ttgggtcgaa cgctggatcg ttcaaaaata
     2581 caccctaacc ccaactatcc gaaatcggat agctgggaag gattgcggat gggaacgaac
     2641 gcgcgatctc gtcagacttg ttcccgtgaa gcccggactt cttcgaggga ttcgtcgacc
     2701 agggcccgaa gatcatcgat ggacccatct gtttttttgc tgtcggcgct ctgcgtcttc
     2761 cgacgtttca tctcggcttc gaactgagag aaaccatcct cgtcgaacac ttccagattc
     2821 ctttttttct ttcctcccaa ccccagaacc cgcgcaagcc aaggcttggc ttcttggact
     2881 tcgagcatct cgtcgcgtgc gacctcggcc gggtcacggc gcaatcggcg aatcaatttt
     2941 tgattcgagt ccacccacag cttggcctca ctcatagcgg ctcgttgtgt ggggtcgagg
     3001 gtattggtaa acgacgacgt gatttcgtga tgagccacga atatcgcaag gccaagagcg
     3061 atttccgtga cttgcaaggc ggtggcccga tcgacgtgaa cgccgccgca acctttcgaa
     3121 aacatataag aagcattggc gagcagcaca ccaaaaccca gggtgatggc ggctagctca
     3181 caggtcgaac gccattcatt ttccggccac gattcatcga atcccccggt ctcttcgata
     3241 tgaattcgcg ccaaggcggt ggcaagataa gcggtcaacg tctcaggctt tcgcgcctgg
     3301 ctgagcaata catccac
//
LOCUS       DAOSOR010000631         3316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_28864_length_3316_cov_3.732904, whole genome shotgun sequence.
ACCESSION   DAOSOR010000631 DAOSOR010000000
VERSION     DAOSOR010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28864_length_3316_cov_3.732904"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..859
                     /locus_tag="PK710_22775"
     CDS             188..859
                     /locus_tag="PK710_22775"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT12623.1"
                     /translation="MRKDDGSFEPHSPYERPQPNVRYQARSEPGVFPQAYINRHESQA
                     ACEAAGKRLCSLAEWYRACRGPRGFTYPYGPQRIRGACNASKHHLLSRIYGTNPRAWK
                     YDEHFNDPKLNQEPGFLAKTGEYEACISEYGVTDMVGNLHEWISDTVDPSLENKIPLR
                     DDIRKKLEKNTGHVIFMGGFFSTTAEHGNGCAFITIGHEPKYHDYSTGFRCCKNASAP
                     PASVP"
     gene            complement(862..2265)
                     /locus_tag="PK710_22780"
     CDS             complement(862..2265)
                     /locus_tag="PK710_22780"
                     /inference="COORDINATES: protein motif:HMM:NF037014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12624.1"
                     /translation="MAKGGKGEGVDRAARVQPFVFTCYLFSSGFDSSRIGLWASEGLS
                     VGMQAGYALRVLHSMLFANIRNLAGARTLAHSSAGNRRACFEVAVGAFPRHWGFLVGT
                     DPGGFATALENAGPSGAEIEEALRARWIDDEFAERLEEPFRLDVNAPPDGSLLFERDP
                     VVTVEGPLWQAWLAAHWARAWFELPCVVATRAARLTMAAGKMGVMDATSSSIVDPELA
                     VRVARAAHVGGLRSTQNAVAASRLGIKLRITKPPEALSLAEEAEVVADRGWTFRSTAV
                     GAVAHLGPGDDEEETLADLQRHGLQAAEWASRGIAILPVGLRLRVDLVAMEQDGVWVS
                     RLGAIPDMTVIPGRKLVVRYFDRTGQPIADLMHGVAERVSPPEEAMLMGHLDVPTSIP
                     IVGATTCKPVLEPLLRGGHGVGMGSSVDKARAELRSALQMLPAAYARLRYPARFPVGY
                     TRVLADMKASLVAMHAS"
     gene            complement(2298..2702)
                     /locus_tag="PK710_22785"
     CDS             complement(2298..2702)
                     /locus_tag="PK710_22785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12625.1"
                     /translation="MGAIKNEAACGGSEPPASESRPVRPGQETKSSMPAPDPNRPSGW
                     FGAPIYRSPVLPPLETEHKTFELRTVRLFSEFETKAPSPGLTDGRKQMDIPTVPTVRV
                     QRRRLWVRLLWLQGWGIVVGIGILLYFWLTQP"
     gene            2798..>3316
                     /locus_tag="PK710_22790"
     CDS             2798..>3316
                     /locus_tag="PK710_22790"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOT12626.1"
                     /translation="MARRPPFNRKSLLQALDLAQLQLVAARLGIQLSPASHAQRLRSK
                     LACHPDATARRILEVLDNDTVASLFDRIGFEAPAPPSPADHPRTRESRFVAIDFETAD
                     SKPDSACSVALVRVHGQRIVKRAARLIRPPRRQITLTWVHGITWQDVAEAPTFRAVWL
                     ELAPILEGTDFLV"
BASE COUNT          723 a         1213 c          759 g          621 t
ORIGIN      
        1 gcccacgccc agcgcatcgc cgcccccaag cgccacccat gccctacctt ccgcctcggc
       61 agcgaattcc accgcttccg ccgcgacttc ttcgagcgcg ccatccgcgg aatccgctcc
      121 gccctgcccg ccggaaatgg cccatatcca tcgaacttgc atcgataaat gggaagccca
      181 tctcgtcgtg cgcaaagacg atggctcgtt cgaaccccac tccccgtacg aacgtcctca
      241 gccaaatgta cgttatcaag cacgctccga accaggcgtg tttcctcaag cgtatatcaa
      301 tcgtcatgag tcccaagccg cctgcgaagc ggcgggcaaa cgcttatgct ccttggcaga
      361 atggtatcgc gcctgccgtg gcccccgagg ctttacctat ccctacggcc ctcagcgaat
      421 ccgaggcgct tgtaacgcaa gcaagcacca cttgttgtct cgcatctacg gcacgaaccc
      481 gcgcgcgtgg aaatacgacg agcacttcaa cgatccaaaa ctgaaccaag aacctggttt
      541 cctggcgaaa acgggcgaat acgaagcctg tatcagcgaa tatggcgtga ccgatatggt
      601 cggtaatctc cacgagtgga tcagcgatac cgtcgatcca tccttggaaa ataagatccc
      661 cttgcgcgac gatatccgca aaaaactcga gaaaaacacg ggtcacgtaa tttttatggg
      721 cggttttttt agtacgaccg ccgagcatgg aaacggttgc gccttcatca ccatcggcca
      781 tgaacccaag taccatgact attcgaccgg atttcgatgc tgcaaaaacg catccgcccc
      841 ccctgccagc gttccctgat ctcaactcgc atgcattgcc accaaactcg ccttcatgtc
      901 cgccaacaca cgcgtatagc ccaccggaaa tcgcgcggga taacgaagac gggcgtatgc
      961 cgctggcagc atctgaagcg cgcttcgtaa ctccgcccgc gctttgtcca ccgaactgcc
     1021 catccccacc ccatgccctc cacgcaacaa cggctccagc accggcttgc acgtcgtcgc
     1081 acccacgatc ggaatgctcg tcggtacatc cagatgaccc atgagcatcg cctcttccgg
     1141 gggggatact cgctctgcaa ccccatgcat caagtccgca atcggctgac ccgtacgatc
     1201 gaaataccga accacaagtt tgcgaccagg gatcaccgtc atgtcaggaa tcgccccaag
     1261 ccgcgatacc catacgccat cctgttccat cgccaccaag tccacgcgca gtcgcaaccc
     1321 gacgggcaat atcgctatgc ctcgactcgc ccactctgcc gcctgaagcc cgtgccgctg
     1381 caaatccgcc aacgtttcct cttcatcatc cccaggacca aggtgcgcaa ccgcccccac
     1441 cgccgtcgac cgaaacgtcc accccctatc ggccaccacc tcggcttctt ccgccaaact
     1501 caacgcctcc ggcggcttgg tgatccgaag cttgatcccc aaccgagatg ccgccaccgc
     1561 gttttgcgtc gaccgtaacc cccccacatg cgccgcccgc gccacacgaa ccgccaactc
     1621 cgggtcgaca atcgaagacg acgttgcatc catcaccccc atctttcccg ccgccatcgt
     1681 caaacgcgcg gctcgcgtcg ccaccacaca cggcagttcg aaccatgccc tcgcccaatg
     1741 ggccgccaac cacgcttgcc acagcggccc ttccaccgtt accaccggat cccgctcgaa
     1801 gagcaaactg ccatccggtg gcgcattcac atccaaacga aacggctcct ccaatcgttc
     1861 cgcaaattca tcgtcgatcc aacgagcccg taacgcttct tcgatctcgg caccactcgg
     1921 gcccgcattc tccaaagcag tcgcaaatcc cccaggatcc gtgcccacca aaaatcccca
     1981 atgccgcgga aaagccccca cggccacctc gaaacaagct cgacgattcc ccgcactcga
     2041 atgggcaagc gtcctcgccc ccgcaaggtt gcgtatattg gcaaacagca tcgaatgaag
     2101 cacccgcaaa gcataacccg cctgcatccc caccgacagc ccttcgcttg cccagagccc
     2161 aatccgggat gaatcgaatc ccgagctaaa taaataacat gtaaatacaa atggttgtac
     2221 cctcgccgcc cgatcgactc cttccccttt ccctcctttt gccaccgggc caatcgctgc
     2281 ccatcgagcc ggcttcctta gggttgggtt agccaaaaat acaataaaat tccgatacct
     2341 accacgatcc cccatccctg caaccacagc aatctcaccc ataatcgtcg cctctgcacg
     2401 cggacggtcg ggaccgtggg gatgtccatc tgtttgcggc catcggttag ccccggggag
     2461 ggtgctttgg tctcgaattc ggaaaacaat cgaacggttc gcagttcaaa ggttttgtgc
     2521 tccgtttcga gcggtggcaa cacgggactc cgatagatgg gagcaccaaa ccagccggaa
     2581 ggtcggttcg gatcgggcgc tggcatcgaa cttttcgttt cttgcccagg acgaaccggc
     2641 ctcgattcgg aagcaggggg ttccgatccg ccgcacgctg cctcgttttt tatcgcaccc
     2701 atggcggtca actccacccc cgtggtactg cacccatgga ttccggccaa aaaatcggat
     2761 cttgccttgc ccaatcgcta ccatcccacc cactgccatg gcgcgccgtc cccccttcaa
     2821 tcgaaaatcc cttctccaag cgctggatct cgctcaactc cagctcgttg ctgcgcggct
     2881 cggtatacaa ctatcccctg cgagccacgc acaacgtttg cgttccaaac tggcctgcca
     2941 tcccgatgcc accgcccgtc gtatcctgga agttctcgac aacgataccg tcgccagcct
     3001 tttcgatcgg attggcttcg aagcaccggc tccaccatcg cccgccgatc atccgcgcac
     3061 ccgcgaatct cgcttcgtcg ccatcgactt cgaaaccgcg gattcgaaac ccgatagcgc
     3121 atgctccgtc gctctggtcc gcgtacacgg ccaaaggatc gtgaaacgtg cagcccgtct
     3181 catccgacct ccccgacgcc aaatcacact cacgtgggtg catggtatca cgtggcaaga
     3241 cgtggcggag gcgcccacct ttcgcgctgt ttggctcgaa ctcgcgccca tcctcgaagg
     3301 gacggacttt ctcgta
//
LOCUS       DAOSOR010000632         3304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_29002_length_3304_cov_5.514928, whole genome shotgun sequence.
ACCESSION   DAOSOR010000632 DAOSOR010000000
VERSION     DAOSOR010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29002_length_3304_cov_5.514928"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PK710_22795"
     CDS             complement(<1..187)
                     /locus_tag="PK710_22795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12627.1"
                     /translation="MHPAFHRATVLFTTMRSFDYGRSSMEPVVEDRSVHHALVAKIMQ
                     EWVPFNAYLGIRVVRVTE"
     gene            243..1625
                     /locus_tag="PK710_22800"
     CDS             243..1625
                     /locus_tag="PK710_22800"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HOT12628.1"
                     /translation="MHEIRWSLKLASIVGVALALGGWTHSESIAAVGTSSVSQGSPLA
                     YDDWFTQEAMRVDLFHSGVDTESAYSLDEIVWEPLWPGTRRYLIDPFDFGTYRFRVTD
                     RATSQEIFRLGFATLFEEWTGTGEAKNKIRRTMHESIRFPWPKQPVTVTIDHRSRNGK
                     FEPMYRLDIDPRSHQILRVRKHTDFEVVSLTPEAKDPAHCLDIVIIPEGYAREDDSKL
                     RADLQRFTEAFVSTPPWTTMKDMVCIRGIVAYSNASGVSEPRKGVFVDTLLGASFNTF
                     DSPRYLTLQHTKTMREVASLAPYDAVFVMVNSARYGGGGVFNQWAVFTSDNEYDDYVM
                     LHEFGHSMAGLGDEYYDSAISMDEDTMYPPGVEPWEPNITAFVGQRRTAIPWADLIEE
                     PTPVPTPETEEYASVVGLFEGAGYKAKGLYRPQADCKMFHKGLVPFCHVCARSIQRMA
                     RYHAGMELNP"
     gene            1622..2704
                     /locus_tag="PK710_22805"
     CDS             1622..2704
                     /locus_tag="PK710_22805"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12629.1"
                     /translation="MNTRRALSWLALPIALFAYAGCQKTKWKDYDPTASPSANESAAP
                     LPSVLPDPVEALHQRTYAIEVHDDLVHAGTTAGVVSWNLSDPTVPKRVASLTLRGSVQ
                     HLAQMPDTSLLVVSTGPSGVALVDTKEAREGKLTLLNPHPWPTELRKACHAAWHAALL
                     DSKTAVVACGGSGVIRLDVSDPTAPVLKKQVLIPGYARDIAVLGKKGVVAAGRKGVAL
                     IDFGNAVPRVMEQLDLNADARAVAIHDQVAYVAAGERGLVMVDISHAPLRILGELLPK
                     TTDMARGIALSESHAFLCLGDSGLAVIDITDPAKPTELSRFDPKGAVNRATRNGSQLL
                     IANDAYGVAVLDVSNPKTPALMTRER"
     gene            complement(2811..>3304)
                     /locus_tag="PK710_22810"
     CDS             complement(2811..>3304)
                     /locus_tag="PK710_22810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12630.1"
                     /translation="FNDKFYIFLEAASTNGGARTVINFTECRDPNSYHDFTCSAAQAA
                     NDLCHEGDRFCYVAINTALSDCCWYQGCPNKTKPNTDISGTGFSCGTEQVDGLGDGSM
                     GHTYGSSTGWLKTEWPIEPNEEFTLIFHIHDTADSLFDSEVILDGFTFLTQVDKGTAP
                     VVK"
BASE COUNT          711 a          960 c          909 g          724 t
ORIGIN      
        1 cttcggtaac ccgcaccacc cgaatgccaa gataggcatt gaaaggcacc cattcttgca
       61 tgattttcgc gaccagcgcg tgatgtacgc tccgatcttc cacgacaggt tccatcgacg
      121 agcgaccgta gtcgaacgaa cgcatcgtcg taaataggac tgtcgcccga tggaacgctg
      181 ggtgcattgg cggcaaacca tgaggtatag tctgggcctg ttcatcttcg agaggcgtgg
      241 atatgcatga aatccggtgg tctttgaagc tggcaagcat cgttggcgtg gcgcttgcgt
      301 tgggtggatg gacccattcc gaatccatcg cggcggtggg cacttcctcc gtctcccagg
      361 ggtcacccct cgcctatgac gattggttca cccaggaagc catgcgcgtg gacttgtttc
      421 attccggtgt ggataccgaa agcgcatata gcctcgatga gatcgtctgg gagcccctct
      481 ggcctggaac caggcggtat ctgatcgatc ccttcgactt cggcacctat cgttttcgtg
      541 tgaccgatag ggcgacttct caagaaatat tcagactcgg cttcgctacc ttgttcgaag
      601 aatggacggg cacgggggaa gcgaaaaaca aaattcgccg caccatgcat gaatccatcc
      661 gctttccgtg gcccaaacaa ccggtcacgg tgacgatcga tcatcgatca cgcaatggca
      721 agttcgaacc gatgtatcga ttggatatcg atccacgctc ccatcagatc cttcgcgttc
      781 gtaaacatac ggatttcgaa gtggtttcat tgactcccga ggccaaggat cccgctcatt
      841 gcctcgatat cgtcatcata cccgagggat atgcgcgcga ggatgactcc aaacttcggg
      901 cggatttgca acgtttcacg gaagctttcg tttcgactcc tccgtggacg acgatgaaag
      961 acatggtttg cattcgcggt atcgttgcgt attccaacgc atcgggagtg agtgaacctc
     1021 gaaagggtgt gtttgtggac acgcttttgg gcgcgagttt caacacattc gattcgccgc
     1081 gatacctgac gctccagcac accaagacca tgcgcgaggt tgcttccctg gcaccttacg
     1141 atgccgtgtt cgtcatggtg aattccgcgc gctatggcgg cggcggcgtc ttcaaccaat
     1201 gggccgtctt cacctccgac aacgaatacg atgattacgt catgctgcac gagttcggcc
     1261 attccatggc gggactcgga gatgaatact atgattccgc catctcgatg gatgaggata
     1321 cgatgtatcc gccgggggtc gagccgtggg aacccaatat caccgcgttc gtagggcaac
     1381 gacgcacggc tattccctgg gccgatctca tcgaagaacc aacgcccgtg cccacccccg
     1441 aaaccgaaga gtacgcctcg gtggttggct tgttcgaagg cgctggctac aaggcaaaag
     1501 gtctgtatcg cccccaggcc gattgcaaaa tgtttcataa aggcctagtc cccttttgcc
     1561 acgtctgcgc tcgaagcatc caacgcatgg cgcgatatca cgcgggaatg gagttgaacc
     1621 catgaacact cgtcgtgcgc tatcgtggct cgccctgccc atcgcgcttt tcgcttacgc
     1681 cggatgccaa aaaaccaagt ggaaagacta cgatccaacc gcttccccct ctgccaacga
     1741 gtccgccgcc ccgctgccca gcgtgctgcc cgaccccgtc gaagctttgc accaacgcac
     1801 ctacgccatc gaagtccacg atgacctcgt tcacgcgggc acgaccgctg gtgtcgtgag
     1861 ctggaatcta tccgatccaa ccgtacccaa gcgggtagcg agccttaccc ttcgaggaag
     1921 cgttcaacat ctcgcgcaaa tgccggatac atcgctactc gtcgtttcca cgggcccttc
     1981 cggagttgcg ttggtggaca cgaaagaggc gcgagaagga aaactcacgc tgctcaaccc
     2041 ccatccatgg ccgaccgaat tgcgcaaggc gtgtcatgcc gcatggcacg cggcgctttt
     2101 ggattcgaaa accgcagtgg tggcgtgtgg tgggtcagga gtgattcgtc tggacgtgtc
     2161 ggatccaaca gcgcccgtgc tgaaaaagca ggtgctaatt ccaggatatg cacgtgatat
     2221 cgcggtgtta gggaagaaag gcgtcgtggc cgcgggccga aaaggcgtgg cgctcatcga
     2281 ctttggcaat gccgtccctc gcgtgatgga acaactcgac ttgaatgccg atgcgcgagc
     2341 ggtggcgatt cacgaccagg ttgcctatgt ggccgctggc gaacgagggc tcgtgatggt
     2401 ggatatttct cacgctcccc ttcgaatcct aggagaatta ttaccaaaaa cgacggatat
     2461 ggcgcgaggc atcgcgttgt ccgagtccca tgccttttta tgcctcggcg attcgggtct
     2521 tgccgtgatc gatatcaccg accctgcgaa acctactgaa ttgagccggt tcgaccccaa
     2581 aggcgctgtc aaccgagcga cgagaaatgg atcccaattg ctgatcgcca atgatgccta
     2641 cggtgtggcg gtcttggatg tgtcgaatcc gaaaacaccc gcgctgatga cacgcgagcg
     2701 ctagggtatt tcatggggcc cgagcaatgg gcgcgtgttt ggcgcgcttg gcagcgtggg
     2761 tgatgagcct gcgacgttga atcatttttc ccttccacac cctttgcgat tcacttgacc
     2821 actggcgccg ttcccttgtc gacctgcgtc aaaaacgtga acccatcgag aatcacctca
     2881 gagtcgaaca aactatccgc cgtatcgtgg atgtggaaaa tcagtgtaaa ctcctcgttg
     2941 ggctcgatcg gccactcggt cttgagccag cccgtcgaag acccatacgt atgccccatg
     3001 ctcccatccc caagaccgtc gacttgctcc gtgccgcatg aaaaacccgt acccgaaata
     3061 tccgtattgg gtttggtctt gttgggacag ccttgatacc agcaacaatc cgatagcgcg
     3121 gtgttgatcg ccacgtagca gaaacggtcc ccttcgtggc aaaggtcgtt ggccgcctgc
     3181 gccgcgctgc acgtaaagtc atgataacta ttgggatccc gacattccgt gaagttgatc
     3241 accgtgcgcg cgccaccgtt cgtgctcgcc gcctcgagga aaatataaaa cttatcgttg
     3301 aact
//
LOCUS       DAOSOR010000633         3300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_29049_length_3300_cov_3.751002, whole genome shotgun sequence.
ACCESSION   DAOSOR010000633 DAOSOR010000000
VERSION     DAOSOR010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29049_length_3300_cov_3.751002"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="PK710_22815"
     CDS             <1..1104
                     /locus_tag="PK710_22815"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOT12631.1"
                     /translation="EQNGNPSVIGGGIRAKSELLVEDSIVRDNVGDRGSGIASGPARA
                     IIARTAIQANTSHGDHGGGLYAAGPVDVVGCLVVDNTVGKTAGYGWGGGLMFFNEGTK
                     ATVTDTEVRGNFAPTKGSGVFVDDGAEASLRNVLIVNNRCAKEGGALYVDGATEQLGS
                     KAWLTNVTIAGHDCGDNGYGNAVVMERASTLEVVSSIFWNNGPRDTSVQPGNTLTVRY
                     SNTKDKLPGPGNLSADPLFASAQTGDYRLKNQAGRWDPSLNQCVKDDVTSPCVDKGDP
                     DGSYEREPKPNGGRVDMGAFGNSPLAGCAPEQSLTTAAPGSNTPSLGPSRSGAAKGRC
                     GCEAPGSRRGQWGWLGVALSWLAFRTRRKPFRR"
     gene            1007..2905
                     /locus_tag="PK710_22820"
     CDS             1007..2905
                     /locus_tag="PK710_22820"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOT12632.1"
                     /translation="MKPREAVEASGGGLGWRSHGSLFELAANRSVDDKARRSQPFALD
                     STAMKASLPFVSVAVLLLTYSSAQAGDLHVDARNSGSEDGTASHPYRSVQAAVSAAKD
                     GDRIKIAGGTYSETISVTNKGIVFEGGYPGASSYDQGGSGDFNQADPGKHPTILQGSS
                     GSAVLNVSGTVQTIVDGLVLRDGRFGITSDAYPELKGTVTVRRSLIENNGSASSQGGG
                     IRAESALVVEDSVIRGNVGERGSGISARKASLRLVRSLVEDNESRGDHGGGVYAAGDV
                     EVIGCVIRNNRVGEGLNYGWGGGIIVFNAGTNATIADTEVLGNYAPTKGSGIFVDDGA
                     RATIRNVLVANNQCSERGGAVYVDGLDAQHGSRATLINVTVAGHACGDGRGNALLLEQ
                     GSEVTVESSIFWGNGAKDVTVDPGNSLKVRYSNSIDNLSGDGNLSVDPLFANPGKGDY
                     RLKNEAGRWDPQSESCVKDSETSPCVNAGDPSGAFDREPEPNGGRVDMGAYGNSLSAG
                     CAANGGAGGFGGAGGTAGQGGTGGSGPAGGSAGGPGGSGGSAEEGGGAGSGGGGGGTG
                     GTGQAGTGAQAGSGLGGMAATTSSRNDDDGCGCRFVHREANMSMVWLTVLGLLGWRAR
                     RKYSKIIN"
     gene            complement(2914..>3300)
                     /locus_tag="PK710_22825"
     CDS             complement(2914..>3300)
                     /locus_tag="PK710_22825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12633.1"
                     /translation="ETLASLGNRDPWLRHRSHQMLDRAQELRILLPTLRHFAHDELER
                     ARINTLEARFDATLGSLDPTSRALQLAAFGIANAHALRCRFRRDLDQPRFSRTRYRWP
                     SPMHRWQQSLESLAAGLRKPSEQPIG"
BASE COUNT          678 a          887 c         1104 g          631 t
ORIGIN      
        1 gaacaaaatg ggaacccgtc cgtcattggc ggcggcatcc gcgccaaatc cgaattgctc
       61 gtggaagact cgatagttcg tgacaacgtg ggagaccggg gctccgggat cgcttcaggt
      121 cctgcccgcg ccatcattgc gcgaaccgcc attcaagcca acacctccca cggggaccat
      181 ggaggagggc tgtacgcggc aggtcccgtc gatgtggtag gctgcctcgt tgtcgataat
      241 actgttggga aaacagcagg ttacggatgg ggcgggggat tgatgttctt caacgaagga
      301 accaaagcca ccgtgaccga caccgaagtc cgtggcaatt tcgcccccac gaaaggcagc
      361 ggcgttttcg tcgacgacgg tgccgaagcc tcgttgcgca acgtgctcat cgtcaacaac
      421 cgatgcgcca aagagggtgg tgccctgtat gtggacggcg ccaccgagca gttgggctcc
      481 aaagcctggc tcaccaacgt caccatcgcg ggacacgatt gtggcgacaa cggctatggc
      541 aatgccgtcg tgatggaacg agcctccacg ctggaagtcg tgagcagcat tttttggaac
      601 aacggcccca gggacaccag cgtgcagccc ggaaatacgc tgacggtgcg ctactccaac
      661 acgaaagaca aactcccggg cccaggcaac ctgagcgcgg acccgctctt cgcctccgcg
      721 caaaccggcg actatcgttt gaaaaatcag gccggtcgct gggatccgtc gttgaatcaa
      781 tgcgttaaag acgacgtaac cagtccgtgt gtggacaagg gggatcccga cggttcgtat
      841 gaaagggagc ccaaacccaa tggtggccgt gtcgatatgg gagcttttgg caattcgccg
      901 cttgcgggct gtgcgccgga gcaatcgttg acgaccgctg ctcccgggtc gaatacccca
      961 tcgctcggtc cctcccgctc cggtgcggcc aaaggacgat gcggctgtga agccccggga
     1021 agccgtcgag gccagtgggg gtggcttggg gtggcgctct catggctcgc ttttcgaact
     1081 cgccgcaaac cgttccgtcg atgacaaggc tcgacgctcc cagccgttcg cgttagattc
     1141 cacggcgatg aaagcttcct tgccattcgt ttccgtggcg gtgctcttgc tcacctattc
     1201 ctcggcccaa gccggcgacc ttcatgtcga tgctcgtaat tccggctccg aagatggaac
     1261 cgcatcgcat ccgtatcgaa gcgtacaagc ggcggtcagc gcggccaagg atggagaccg
     1321 tatcaaaatc gcaggcggaa cctattccga aaccatcagc gtcaccaaca aagggatcgt
     1381 tttcgaagga ggatacccag gagcgtcttc gtacgatcaa ggcggttccg gagatttcaa
     1441 ccaggccgat cccggcaaac accccacgat tttacaaggc tcttccggtt ccgcggtgct
     1501 caatgtctcc ggaaccgtgc aaaccatcgt cgatggactc gtgcttcgcg acggacgatt
     1561 cggtatcaca tcggatgctt atccggaact caaaggcacc gtgacggtgc gccgatctct
     1621 catcgaaaat aacggtagtg catcgtccca gggcggaggg attcgcgcgg aatccgcgtt
     1681 ggtggtcgaa gatagcgtga ttcgaggcaa tgtaggggag cgggggagcg ggatttcggc
     1741 tagaaaagca agccttcgac tcgttcgaag tctggtggag gataatgaat cccgcggtga
     1801 tcacggggga ggcgtgtatg ccgcgggcga tgtggaagtc atcggatgcg tcatccgcaa
     1861 caaccgggtt ggtgagggtt tgaactacgg ctggggtggc ggcatcatcg ttttcaacgc
     1921 ggggacgaac gctaccattg cggatacgga agtgcttggc aactatgccc ccacgaaagg
     1981 aagcggcatt ttcgtcgacg atggcgctcg tgccacgatt cgcaacgtgc tcgtggccaa
     2041 caaccaatgc agtgaacgcg gaggcgcggt ctatgtcgat ggtttggatg cacagcacgg
     2101 ttctcgcgcc actctcatca atgtcacggt ggcgggacat gcctgtgggg atggtcgggg
     2161 caatgcgttg ctgctcgagc aaggctccga agtgacggtg gagagcagca tattttgggg
     2221 aaatggtgcg aaagacgtca ccgtggatcc gggcaattcg ctgaaggttc gctattcgaa
     2281 tagcattgac aacctttcgg gcgatggaaa tctgagtgtg gatccgctat tcgcgaatcc
     2341 cggcaaaggc gattaccgct tgaaaaacga ggctggacga tgggatcctc aatccgagtc
     2401 gtgtgttaaa gacagtgaaa ccagtccgtg cgtgaatgcg ggggaccctt cgggagcctt
     2461 cgatcgagag cccgaaccga atggcgggcg cgtggatatg ggcgcttatg gcaattcgct
     2521 gtccgcgggg tgtgcggcca atggcggcgc cggtggattt ggcggcgctg gtggtacggc
     2581 agggcagggg gggactggcg gaagcggacc cgcgggtggt tcggcagggg gacctggcgg
     2641 ctcgggaggc agtgcggagg aaggtggcgg tgctggctcc gggggaggtg gcggcggtac
     2701 cggaggaacg ggacaggcgg gcaccggtgc tcaggccggc tcgggactgg ggggcatggc
     2761 cgcaacaacc tcgtcgcgaa acgatgacga cggttgtggc tgcaggttcg ttcatcgtga
     2821 ggcgaatatg tcgatggtgt ggcttaccgt gcttggcctg ctggggtggc gagccaggcg
     2881 caagtattcg aaaataataa actaattccc tacctagccg atgggttgct cgctcggttt
     2941 ccgcaatccc gcggcgagag actctagact ttgttgccag cggtgcatcg gcgagggcca
     3001 gcgatatctc gttcggctaa agcggggttg atccaggtcg cgtcggaaac ggcagcgcag
     3061 cgcatgggcg ttggcgattc cgaaagcggc gagttgcagc gcccgggagg ttgggtccaa
     3121 agagccgagg gttgcgtcaa aacgagcttc gagggtgttg atacgagcgc gttcgagttc
     3181 atcgtgggcg aaatggcgca gcgtaggcag caaaatccgt agttcttgcg cgcgatcgag
     3241 catctggtgg ctccgatgac gcagccacgg atcgcggttt cccagcgagg caagggtttc
//
LOCUS       DAOSOR010000634         3278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_29317_length_3278_cov_3.737512, whole genome shotgun sequence.
ACCESSION   DAOSOR010000634 DAOSOR010000000
VERSION     DAOSOR010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29317_length_3278_cov_3.737512"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="PK710_22830"
     CDS             complement(<1..1101)
                     /locus_tag="PK710_22830"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT12634.1"
                     /translation="MVAVSGCGGTVESPTRIDCSDCTILRGATVFDGQVAAPGTVVLR
                     RDRVQSLVRGPVEIVAGRVLDMDNHTILPGLIDLHVHVRASSGPPAMADPHHDTKDHF
                     KALLRSGITTVLDLGTERHIVFALRQRIQDGLLAGPCLLAAGAAFTPTGGHPCVYGRP
                     GFDLCTFVDGAADVDQSLIELAGEHPDAFKIIIESGTQTHPLPTMSHEVIEALVDRGE
                     KLQVPVIAHISRTADIVDALDAGVRFFAHLPVRDRLDDAVAERLARVGAVVIPTVVVE
                     DARYRASLGPLEEVGADDAKSNISEDVLAAWRDPAMVASWREPKMQQEAQRRRDNVLD
                     NLRICHRAGVRIVAGTDAGNPAVFHGISMHREL"
     gene            1420..2136
                     /locus_tag="PK710_22835"
     CDS             1420..2136
                     /locus_tag="PK710_22835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12635.1"
                     /translation="MVVATVVCTSQAKAQKEPSDEESSSSTNEKLRSFPIIETGPQAG
                     FRTGFAKGMGKLSAQDGELGGGSHGFIPFWLDLGYRVHPNVYVGAYGQFGWVLLRKGV
                     LCDGEYVKCSGQNYRVGASFHYHFIPDGDADPWVGLGAGYEWYFSNVSFATSGVPNMR
                     NVFHGPEWVNVSVGLDIKKNRQGGVGPFVALAVGQYLSRLYSDVNEGEVVDVAESIVA
                     TEMHQWLFFGIQGSFFDSFH"
     gene            2320..>3278
                     /gene="arcC"
                     /locus_tag="PK710_22840"
     CDS             2320..>3278
                     /gene="arcC"
                     /locus_tag="PK710_22840"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOT12636.1"
                     /translation="MTSLDRSHPVAVVAIGGNSLIKDKHHQEVRYQWDAARETCFHIA
                     NMIEKGWTVVVTHGNGPQVGFILRRNEIASSEVHTTPLDAIVADTQGSIGYMLQQSLN
                     NEFVRRGLQRHAFTIVTQVLVDKNDSAFKDPTKPIGSFMDETTAMQMKQAEGWDVVED
                     AGRGWRRVVASPHPVQVVEQDAITAAVQQGWIVIAGGGGGIPVVQTPQGLEGVAAVID
                     KDRLSALLAAAIKADLFVISTAVEKVCLHYGKPNQQDLTHITAEQARQYMNEGHFAPG
                     SMKPKMEAVLQFLDEGGKRAIITNPENLERALLGETGTVIEGH"
BASE COUNT          753 a         1020 c          858 g          647 t
ORIGIN      
        1 aagctcccga tgcatggata tcccgtgaaa aacagcaggg ttgcccgcat ccgtgcctgc
       61 cacgatccgc acgcccgccc gatggcatat ccgaagattg tcaagcacat tgtctcgtcg
      121 acgttgcgcc tcttgctgca tcttcggctc cctccacgaa gccaccatcg cagggtcgcg
      181 ccaagcggct agcacatcct cggatatatt cgacttcgca tcatctgccc ccacttcctc
      241 caggggcccc agcgaagccc ggtatcgcgc atcttccacc accaccgtcg gaatcaccac
      301 ggcacccact cgagcaagac gctcggccac cgcatcgtcc agccgatctc gaacaggtag
      361 gtgggcaaag aatctcaccc ccgcatccag ggcatccacg atatccgctg tcctagaaat
      421 atgtgcaatt acaggtactt gtaacttttc tccgcgatcg accagcgcct cgatcacctc
      481 atggctcatc gtaggcaagg gatgcgtttg ggtccctgac tcgatgatga ttttgaaggc
      541 gtccgggtgt tcgcccgcca actcgatcag gctttggtcc acatcggcag ctccatccac
      601 gaatgtgcaa agatcgaagc ccggtctccc atacacacaa ggatgcccac ccgtcggagt
      661 gaacgctgct cccgccgcaa gaaggcaagg ccctgcgaga agcccatcct gaattcgctg
      721 tcggagcgca aaaacgatgt gtcgctcggt ccccaagtcc aacaccgtgg tgatcccgga
      781 tcgcagcaag gccttgaaat ggtctttggt atcatggtgg gggtcggcca tggccggtgg
      841 tcccgaagaa gcacgcacgt gaacgtgaag atcgatgagt ccaggtaaaa tcgtgtggtt
      901 gtccatatcc agcacccgac ccgccacgat ttcgactggt cctcgaacca gcgattgcac
      961 acggtcccgt cgaagcacaa ccgtgcctgg cgcggctact tgcccatcga ataccgtggc
     1021 tcctcgaagg atcgtgcaat cggaacaatc gattcgtgta ggagattcta ccgtgccacc
     1081 acatccactc acggctacca tggcaagggc ggaacacaac cttaggctgc tgcaaacagg
     1141 catgaagata ggataattca gcttcggttc gcgagcactc caaggattgg cagcgagttc
     1201 atgccagcgt gaggaacccg tacacgaacc atagcccccc gcttgccctc gattggcgcg
     1261 aatcgttcaa gaatacctcg attgctctgg accaagggac acccctcgac gtcagcaccc
     1321 gcaaaggata cactgcggtt ctgtcacacc atcgaaccca tgccgaacac gcattccatt
     1381 ctcatgcggc ggatcgtatt gtcacagtgg ttagcaacca tggtcgttgc aaccgttgtc
     1441 tgcacctcgc aagccaaggc gcagaaggag ccatcggacg aggaatcttc ctcgagcacg
     1501 aacgaaaaac tccgctcctt ccccatcatc gaaacaggcc cccaagctgg ttttcgaacc
     1561 ggttttgcga agggtatggg aaagctctcc gcacaagacg gcgaacttgg cggcggctct
     1621 cacggcttca tccccttctg gctcgatctg gggtatcgcg tccaccccaa cgtatacgtc
     1681 ggcgcctacg gtcaattcgg ttgggtgctg cttcgcaaag gggtgctatg cgacggcgag
     1741 tacgtcaaat gctcgggaca aaactatcga gtcggagcca gcttccatta tcatttcatt
     1801 cccgacggtg acgccgatcc ctgggtcggc ctcggcgcgg gctacgaatg gtatttcagc
     1861 aatgtttcct tcgcgacgtc aggcgtgccc aatatgcgga acgttttcca cggacccgaa
     1921 tgggtcaacg tcagcgtcgg tctggatatc aaaaagaatc gacagggagg agtcggcccc
     1981 ttcgtcgccc tggccgtcgg ccaatatctt tctcggctct attcagacgt gaatgaggga
     2041 gaagtggtcg atgtcgccga atccattgtc gccaccgaga tgcaccaatg gctgtttttc
     2101 ggaatccaag gctctttttt cgacagcttc cactagtcgt atccagggtt gacctcgacg
     2161 ccaactggtt gtacccgagg gcgtctccgc acgcgtaacg attggagttg gtcgtcatca
     2221 ggctggtctg atcacctccc ccgatcaacg cgacgactat cccagcatca cgaccaagcc
     2281 cacccaagct tctgtcggat gactcccgaa ggttggacca tgacctcgct cgatcgctcg
     2341 caccctgtcg cagtggtcgc cattggcggc aattccctca tcaaagacaa acaccatcaa
     2401 gaggttcgct accaatggga tgcggcccgt gaaacctgct tccacattgc caatatgatc
     2461 gaaaaaggat ggaccgtggt cgtcacccac ggcaatggtc cacaagtcgg cttcatcctc
     2521 cgacgaaatg aaatcgcgtc ttcagaagtc cataccactc cgctcgacgc catcgtcgcc
     2581 gatacccaag gcagcattgg ctacatgctt cagcaaagcc tcaacaacga attcgtgcgc
     2641 cgcggcctgc aacgccatgc cttcaccatc gtcacgcaag tgctcgtcga caaaaacgac
     2701 tcggctttca aggatcccac caagcccatc gggtcgttca tggacgaaac gacagcgatg
     2761 caaatgaaac aagcggaggg atgggatgtg gtggaagacg cggggcgagg atggcgacga
     2821 gtggtggcgt cacctcatcc ggtgcaagtc gtcgaacaag atgccatcac cgccgctgtc
     2881 caacaaggtt ggatcgtgat agccggcgga gggggaggta ttcccgtcgt tcaaaccccc
     2941 cagggcctcg aaggagtcgc ggccgtcatc gacaaagacc gtctctcagc tcttttggct
     3001 gccgcgatca aagccgactt gttcgtcatt tccacggcgg tggaaaaggt ctgcttgcat
     3061 tacggaaaac cgaaccaaca agacctcacg catatcacgg cggaacaggc tcgacagtac
     3121 atgaacgaag gccattttgc gcccggttcc atgaaaccaa aaatggaagc cgtccttcag
     3181 tttctcgacg aaggaggcaa gcgcgccatc atcaccaatc cagaaaacct cgaacgcgcg
     3241 ttgctaggcg aaaccggcac cgtcatcgaa gggcattg
//
LOCUS       DAOSOR010000635         3266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_29440_length_3266_cov_4.183743, whole genome shotgun sequence.
ACCESSION   DAOSOR010000635 DAOSOR010000000
VERSION     DAOSOR010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29440_length_3266_cov_4.183743"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PK710_22845"
     CDS             <1..252
                     /locus_tag="PK710_22845"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOT12637.1"
                     /translation="GIGSYGTVGLDFAVAVTIGLLGGWWLDKKVGWTPWLTIVGLLFG
                     VAAGFNILFKAAKRLREQTEREERIDSRQRDGNGDSSER"
     gene            224..694
                     /locus_tag="PK710_22850"
     CDS             224..694
                     /locus_tag="PK710_22850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12638.1"
                     /translation="MATVIQASDSQGKELAEAGAQVDVALVAVGGFGLMASLVGALGW
                     DWHVAVGVGVGSGLAVANLWLLKRLARAFFSRGGSSRVLWGLLGVFKFVGLMVGVGLL
                     LRHDIVGPLPLIVGYGALPFGITLSNFLGARSQADVMECHESGSCDSFDSTKKG"
     gene            704..1516
                     /gene="atpB"
                     /locus_tag="PK710_22855"
     CDS             704..1516
                     /gene="atpB"
                     /locus_tag="PK710_22855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOT12639.1"
                     /translation="MPEHTSFLTYLVYMLPNGAELAERFGHGVVANTPPSWQSWEPPV
                     TALLLVLILLALGYRARATFTKLDDAVIPEDKLSIRTFFEVFLGYFYSMARDVMGPER
                     AKRYFPIIGAASCFVFFGNIVGLIPGLVIPPTSSLNITLGSALLVFVLFNFYGIKANG
                     LGYLKHMCGPWLGPAGIPLNALIFVVEVISTCVRPITLSVRLMLNMAVDHLLAGIFLG
                     LFALLVPVPVMFLGVIVILVQTLVFTLLSSIYIALATEHEEHDHGGREHAHA"
     gene            1669..2004
                     /locus_tag="PK710_22860"
     CDS             1669..2004
                     /locus_tag="PK710_22860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOT12640.1"
                     /translation="MLSKNKLALAVGSLVVLVTTSAFAQDAATNANDVYAWAAAAAGF
                     AIGIAALGGTMSQGRSVSAALEGISRNPGAAPRIQTAMILGLAMIESLVLFALVIAIL
                     LWTKITVGA"
     gene            complement(2104..>3266)
                     /locus_tag="PK710_22865"
     CDS             complement(2104..>3266)
                     /locus_tag="PK710_22865"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOT12641.1"
                     /translation="PIPVHANSVLAIHDEVPVFESPEASAKRRGVLAADIPLPFFAFR
                     RGPGCAGRWIGIGPSAWVCQDKITLSHSQPVPASFSTYRESKNGLPYPYFFVGRRGSW
                     GYVHLRSVDVSMPDQNYEPGFAVAIVQEREHNRELYGRTTRGYWVPMRDLNPARPTTF
                     HGEVVRDGLLDFVWIHSAQARALSKPMAHAAKQDNLVRFQWLRVEEEKIVAGHAYYRI
                     GENRWVSDRDARKPSLAPPPDEIRPGERWIDIHLDSQTLVAYEGDRPVYATLVSTGRG
                     RQNTPFATPFGVHRIWVKLLTSSMGNLQDERASDLYLIEDVPFVQFFHKGVGLHAAFW
                     HDRFGNVRSHGCVNLAPYDAQWLFAFTSPRLPAGWRAILPHEHSESTIVRVR"
BASE COUNT          643 a          827 c         1027 g          769 t
ORIGIN      
        1 ggcatcggca gctacggcac cgtgggactc gattttgcgg ttgccgtgac catcggccta
       61 ttgggcgggt ggtggttgga caagaaggtt ggatggacgc cttggctgac gatcgtggga
      121 cttttgtttg gagtggcagc gggattcaac attttgttca aagcggcgaa gcgattgcga
      181 gaacagacgg agcgagagga acgaatcgat agtaggcaac gagatggcaa cggtgattca
      241 agcgagcgat agtcagggca aagagcttgc cgaggcgggt gcccaggtgg atgtggcgct
      301 ggtggcggtg ggtggtttcg gactgatggc gtcgcttgtg ggggctttgg gatgggattg
      361 gcatgttgcg gttggcgtcg gtgtgggaag tgggctggcc gtggccaatc tatggttatt
      421 gaagcgtttg gcgagagcat ttttttcgcg gggggggtcc tcccgtgtgt tgtggggatt
      481 gcttggggta ttcaagtttg tgggattgat ggttggtgtc ggattgttgc tgcgtcatga
      541 catcgtgggc ccgctgccgc tcatcgtcgg ctatggtgcg ttgccgtttg ggataacgct
      601 gtcgaacttt cttggagcgc gatcccaggc ggacgtcatg gagtgccacg aaagcgggtc
      661 atgcgattca ttcgattcca cgaagaaggg ctgagcgcgg atcatgcctg agcatacttc
      721 atttctgacc tatctagttt atatgctgcc caatggggcc gagcttgcgg agcggttcgg
      781 gcatggggtc gtggcgaata cgccaccttc gtggcaatcg tgggagccac cggtgacggc
      841 ccttttgctc gtattgatcc tgctggcgct tggttaccgc gcacgtgcca cgttcacgaa
      901 gctcgatgat gcggtcattc cggaagacaa gctgagcatc cgcacgttct tcgaggtttt
      961 tctcggatat ttctacagta tggcgcgtga cgtcatgggt ccggaaagag cgaagcgata
     1021 ctttccgatc attggtgcgg catcgtgttt cgtgtttttc ggcaacatcg tcggtttgat
     1081 ccccgggctc gtcattccgc caacttccag tctcaacatc acgttgggtt ccgcgctgtt
     1141 ggtattcgtg ctgttcaact tctatgggat caaagccaac ggcttggggt atctcaagca
     1201 catgtgcggt ccgtggcttg ggcccgcggg tatccccctc aacgccttga tttttgtggt
     1261 cgaagtcatc tccacttgtg ttcgaccgat cacgctgtcc gtacgtctga tgctcaatat
     1321 ggcggtcgat cacttgcttg cgggtatctt tttggggctg tttgcattac tcgtcccggt
     1381 gcccgtgatg tttctcggcg tgatcgtcat cctggtgcag actcttgtgt tcacgctgct
     1441 gtcatcgatc tacattgcgc tcgccaccga gcatgaagag catgatcacg gaggtcgtga
     1501 gcatgctcac gcgtagtcga aaacctgctg accaagtcgg caatggtagg tagttgaagg
     1561 cgcatggttc gaggtccctc gaccctgcgt gaaaaccgtc aaagagcgga gaaggccacg
     1621 gggaatcatg ctcactcccc gtggtgtcat ggaaaggagc gatgttggat gttgtcgaag
     1681 aacaagctgg cgcttgccgt aggatctctg gttgtcctcg ttactacgtc cgcgtttgcc
     1741 caggacgcgg caacgaacgc caatgacgta tatgcgtggg cggcagccgc ggcgggcttt
     1801 gccatcggca tcgcggcgct tggtggcacc atgagccaag gcaggtccgt atctgctgct
     1861 ttggagggaa tttctcgtaa ccccggggct gccccgcgca ttcagaccgc catgatcctc
     1921 ggcctcgcga tgatcgaatc gctggtgttg tttgccctcg tcatcgccat tttgctttgg
     1981 accaagatta cggtcggcgc ataaacccac gacgggcaaa gacggataga tcgtctccca
     2041 gggcccttga ttcgggcatg gggagacgtg tgtcccgcgt gtccatccca ggatcgctcc
     2101 gaatcaccga acgcgaacga tggtgctttc ggaatgctca tgggggagga ttgcgcgcca
     2161 tcctgcgggc aaccgtggac tggtgaaagc aaacagccat tgggcgtcat aaggcgcgag
     2221 attgacacaa ccgtggctac gcacgttgcc gaacctgtca tgccaaaacg ccgcatgcag
     2281 tccgacacct ttgtgaaaaa actgcacgaa gggcacgtct tcgatgagat ataagtccga
     2341 cgctctttcg tcttgcagat ttcccatgct cgacgtcagg agtttgaccc agattcggtg
     2401 aacaccaaag ggggtggcaa aaggtgtgtt ttgacggcca cggcccgtgg acaccaaggt
     2461 ggcatacacg gggcgatcgc cttcgtaggc aacgagggtt tggctgtcga ggtgaatgtc
     2521 aatccatcgt tccccaggac gaatttcatc cggcggtgga gcaaggctgg gctttcgagc
     2581 atctcgatca ctgacccaac ggttttctcc gatgcggtaa tacgcgtgtc ccgcgacgat
     2641 tttttcctct tcgacacgaa gccattggaa acggaccagg ttgtcttgtt tggcagcgtg
     2701 agccatgggt ttcgacaaag ctcgagcttg ggccgagtgg atccacacaa aatccaagag
     2761 gccatctcga acgacttccc cgtgaaatgt cgtgggtcga gccggattca gatctcgcat
     2821 gggcacccaa tacccacgag tcgttcgacc atatagttca cggttatgct cacgctcttg
     2881 gacgatggcg acggcgaagc ccggttcgta gttttgatcg ggcatcgata catccacgga
     2941 tcgcaagtga acgtatcccc aagagccacg gcgtccgacg aagaagtatg ggtagggcaa
     3001 accgtttttg gattctcggt aggtcgagaa agaggcgggg accggttgag aatgggatag
     3061 cgtgattttg tcttggcaga cccaagccga agggccaatg ccaatccatc ttcccgcgca
     3121 tccaggacca cgtcgaaacg caaaaaaagg caatggaata tcagccgcga gcacaccacg
     3181 tcgcttggca ctcgcctcag ggctttcgaa aaccgggacc tcgtcgtgga tggccagcac
     3241 ggagttggca tgaacgggaa tgggaa
//
LOCUS       DAOSOR010000636         3260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_29516_length_3260_cov_3.670515, whole genome shotgun sequence.
ACCESSION   DAOSOR010000636 DAOSOR010000000
VERSION     DAOSOR010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29516_length_3260_cov_3.670515"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PK710_22870"
     CDS             complement(<1..341)
                     /locus_tag="PK710_22870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12642.1"
                     /translation="MRPVVRAAVAVAGLSAISAWSYRFSVPRHITVVTTIDNHDGSPL
                     SSLCPAGFLPDSTQCVPFPPMGDENQQPGLSKGVNTPRDPSGTWKIYEQIPRRPDRPA
                     PYEAYRFPFDPR"
     gene            429..1106
                     /locus_tag="PK710_22875"
     CDS             429..1106
                     /locus_tag="PK710_22875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12643.1"
                     /translation="MTIKEHVDVQQVLTKIFEAERTLRQQSRKLLQAPHGAVLGQLQT
                     AFSQASQLKDEQEAELRLVCLTRLLRTVPGPGAIDLLIDILGHDNEEARQGAGFVLED
                     IASERLGEVRKGIERALKRLPVGSLALCELPFVILGLDGDVIRMLRPFLSHQDPEAVA
                     AAIDAYVEYADPDAITLLEPLTQDNRRVQVNIESTDEEESVTIADLAADAIDALQEVA
                     SIMASEP"
     gene            1484..2917
                     /locus_tag="PK710_22880"
     CDS             1484..2917
                     /locus_tag="PK710_22880"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HOT12644.1"
                     /translation="MTHPFRSWLCPCFGIMFCVAFVDDAKAQTYDGETPVAASDALVM
                     GDWLFRPYLELRSQAEFRFHPFDVGGTMITTRPSSSPIGGTQAEVEKQWWVLSRGRMG
                     LSAERGYLRAVLELQDARLWGELSPLRLDARDRLPSTGARLAYGEVRGVGSRASFLRI
                     GRQQVVWGDGRLLGASDFSPTGRSLDAVRGQWVLGDFDLEGFSSILVSPGAAPAPMRR
                     ETLGSPEGMGTHLHGLRLAWHIAPWLHLQSNNLGRFARFPTEHLIDPGDLYLTGLRVW
                     GGYVGVSYALEGAYEAGRDAAVGSTRSRSAWAVAANANYETGLWWNLGLGVEGAYATG
                     QKKGESAITRFDPMLPDNRVGRDAMGLVAWSNVIDVGATATLSPHDDAQLVFGYRYLA
                     MAEPSDGWQTADMVFVGEAPNNDESFLGHELDARMRLSPWGPLDVEAGYGLFLTGPGA
                     KNVLEASGRGRPNLQHYGYLQTTFRIP"
     gene            complement(2949..>3260)
                     /locus_tag="PK710_22885"
     CDS             complement(2949..>3260)
                     /locus_tag="PK710_22885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12645.1"
                     /translation="PQLQPQLQPQLQPQLQPQPQLQPPPPTAAYPPVNPRMVETQMLA
                     AIQPPAVGHTGSGLMTPTGAPFVDPDAAPVWLKALALSSVVSALTVLGVLGWLLKNRF
                     Y"
BASE COUNT          673 a          832 c         1074 g          681 t
ORIGIN      
        1 cggggatcga aggggaaacg gtaagcctcg tagggggcgg gtcggtctgg gcgacgcggg
       61 atctgctcat agattttcca ggttccggaa ggatcccgag gcgtgttgac tcctttgctc
      121 aatcccggct gttggttttc atcccccatg gggggaaagg gaacgcactg cgtggagtca
      181 ggcaggaatc cggcgggaca gagggatgac agaggtgagc catcgtggtt atcgatggtg
      241 gtcacgacgg tgatatggcg tggcacggag aagcgatagg accaagcgga aattgcgctt
      301 agtccagcta cggcgacggc agcacgcacc acgggacgca cgagccagag cgtaacgtga
      361 ggagggtcca agggccagtg atcgtttgca ccgttgcatt acccgacggg tggaggtagc
      421 cgtaagctat gaccatcaag gaacatgtgg atgtgcagca ggttctcacg aagatattcg
      481 aagcagagcg taccctgcgg cagcagtctc ggaagttgct tcaggcgccc catggggctg
      541 tactcggtca acttcaaacg gctttttcgc aagccagtca attgaaagac gaacaagagg
      601 ccgaactgcg gctcgtatgt ttgacgagat tgctgcggac ggtaccggga ccgggtgcca
      661 tcgatttgct cattgatatc ctcggtcatg ataacgaaga ggcacgacaa ggagccggct
      721 tcgttctcga agacatcgcc tcggaacggt tgggtgaagt ccgaaagggt atcgaacgcg
      781 cgctcaaacg ccttcctgtg ggcagcttgg ccttgtgcga gctgcctttc gtcatcttgg
      841 gattggatgg tgacgttatc cgtatgcttc gacctttcct ttcccaccaa gaccccgagg
      901 ccgtggcggc cgccatcgat gcctatgtcg aatacgccga ccccgatgcc atcactcttc
      961 tcgaaccgtt gacccaagac aaccgccgcg tgcaggtcaa tatcgaatcc acggatgagg
     1021 aagagagtgt gaccatcgcg gacctggcag ccgacgcgat cgatgcactg caagaagtcg
     1081 ccagcatcat ggcttcagag ccctgaatgg gccgtctcga accccgtgtt cccagcttgc
     1141 cggaaaagct gtaacttatt gaatttactt agtttaattt atgtcgcgac ggaaggaagc
     1201 tcgatgccgc tcgaccattt caacgagggc acttgcttgc cggtcgcaac ggcttgaccc
     1261 ttctggcctg acgaacgtct tgatttcatt cagggcagcg tcgaaaacac ccaccgtcgc
     1321 gacagaccga acgtcccgcg ccatgatgga gccaccgtag gatacgtaag cgtctacggg
     1381 ttatcgtctc tccaagacag accaagccat tccccattcc ccattggcca tcgtattgct
     1441 tacactgccc aaccagacca ctcatgccca cggacacgat ctcatgactc acccgttccg
     1501 ctcctggctg tgcccatgct tcggtatcat gttttgcgta gcgttcgtcg acgatgcgaa
     1561 ggcgcagacg tacgatgggg aaacgccagt tgcggcgagc gatgcgttgg tgatggggga
     1621 ttggttattt cgaccctatt tggaattgcg gagccaagcg gagtttcggt ttcatccctt
     1681 cgatgtggga ggaaccatga taacgacgcg tccatcgagc agtccgatcg gtggaacaca
     1741 agccgaggtt gaaaaacagt ggtgggtgtt atcgcgcggg agaatgggat tgtccgcgga
     1801 gcgaggttac cttcgcgccg tgctcgagtt acaagacgct cgtctgtggg gggagttatc
     1861 gccccttcgt ctggatgcga gagacaggtt accgtcgacg ggagccaggt tggcgtatgg
     1921 ggaagtacgg ggtgtggggt ctcgagcttc ctttttgaga attgggcgac aacaggtggt
     1981 gtggggagac ggtcgattgt tgggagcatc cgatttttct cccacgggac gctctttgga
     2041 tgcggtgcga ggccagtggg tgcttggcga tttcgatctc gaagggtttt cgagcattct
     2101 ggtaagccca ggagccgcac cagcgccaat gcgtcgcgaa acactcggaa gcccggaagg
     2161 catgggcacg catctgcacg gcctacggct cgcttggcat attgcgccat ggctccatct
     2221 tcaatccaac aatctagggc gtttcgcgcg ttttccgacg gagcatttga ttgaccccgg
     2281 cgatctatat ctcaccggtt tgcgcgtatg gggtgggtac gtgggcgtat cctacgcgtt
     2341 ggaaggggca tacgaagcgg ggcgagacgc ggcggttggg tccactcgtt ctcgaagcgc
     2401 gtgggctgtg gccgcgaatg cgaactatga aaccggatta tggtggaacc tgggtctggg
     2461 ggtggaaggc gcgtacgcga caggtcagaa gaaaggggaa agtgccatca cgcgtttcga
     2521 tcccatgctg cccgacaatc gggtgggacg cgatgccatg gggttggtgg cttggtccaa
     2581 cgtgatcgat gtcggggcaa cggcaactct ttcgccccat gacgacgcgc agctcgtgtt
     2641 cggctaccga tacctggcga tggcagagcc gtcggatggt tggcaaacgg ctgacatggt
     2701 atttgtgggg gaagcgccga acaacgacga gtcgtttctt ggacatgaat tggacgcaag
     2761 gatgcggctg agcccgtggg gaccgctgga tgtggaggcg ggctacgggc tgtttcttac
     2821 gggtccgggc gcaaaaaatg tgctcgaagc atccggccga ggtcgtccaa acttacaaca
     2881 ttacggctac ttacaaacga cctttcgtat tccttgaaag cgaggagggt cagggcgcca
     2941 caagccggtc aataaaaccg gttcttgagc agccatccga gcacgcccag cacggtgaga
     3001 gcggatacga cgctggacaa ggcgagagct ttgagccaga ccggcgcggc gtcggggtcg
     3061 acgaaggggg caccggtggg cgtcatgagt ccagaacccg tatgaccgac ggctggtggt
     3121 tggatggctg ccagcatctg ggtttcgacc attcgtgggt tgacgggtgg gtaggcggcg
     3181 gtcggaggag gaggctgtaa ttgaggctgg ggttgaagct ggggttgaag ctggggttga
     3241 agctggggtt gaagctgggg
//
LOCUS       DAOSOR010000637         3259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_29528_length_3259_cov_4.655743, whole genome shotgun sequence.
ACCESSION   DAOSOR010000637 DAOSOR010000000
VERSION     DAOSOR010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29528_length_3259_cov_4.655743"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PK710_22890"
     CDS             complement(<1..604)
                     /locus_tag="PK710_22890"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HOT12646.1"
                     /translation="MVISAPFEVFEPRTGHTPVVVEVPHAGLFMDPESMAHCVAPVMS
                     LAADADLYVDELVTDLFEEGATIIASRWSRYVLDLNRAPDDVDGRSVRGATASSCPHG
                     LLWRETRSGCQALRQPVEPQELQRRLDCFYKPYHGALDEVVEHRCQRFGTVVVLSMHS
                     MPSEGVDADVVIGTLGRSTASDRLVDGLEKAVRAFGLRVVH"
     gene            complement(649..1542)
                     /gene="xerD"
                     /locus_tag="PK710_22895"
     CDS             complement(649..1542)
                     /gene="xerD"
                     /locus_tag="PK710_22895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOT12647.1"
                     /translation="MDLHAWVDAYLDYLRIERALSRNTVESYSRDLGKLLALAEEQGA
                     KTPKDIDTPLVSVYLVELGKQGLGARSAARHLSAVRGFCRFLVREKAISTDPCVLIDR
                     PRTRRKLPIWLDEQEVDRLLDAPDESTPRGIRDRAMLRLMYAAGLRVSELVGLRLGAV
                     DFRAGIVKVTGKGGKSRLVPVGEVALDAIQTYLEMGRAHHATEKDTVLFVSPRGGALT
                     RQGFWKLVVRYARGVGIMKPISPHKLRHSFATHLLRHGADLRAVQAMLGHADIGTTEI
                     YTHVADDHVRRVYRETHPRAR"
     gene            complement(1716..2210)
                     /locus_tag="PK710_22900"
     CDS             complement(1716..2210)
                     /locus_tag="PK710_22900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12648.1"
                     /translation="MMVSRALLLLMLGLLPGCGRGGMASCEQDRRQAQAVLEKDKRVA
                     RISHEADQLALQGRPRQAADRIEADAKIPMDEAISAAKAWKPQTVWGAERAKDLLALT
                     EQRRQRLTAYVTALRSEQLTKVVEQMELQRDLEKQALEVEKTLSAGPQTADACAILPD
                     STGQ"
     gene            complement(2207..2515)
                     /locus_tag="PK710_22905"
     CDS             complement(2207..2515)
                     /locus_tag="PK710_22905"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOT12649.1"
                     /translation="MERRVSKRTRVDLLINKYIDGYPYACRVMDISKGGVLLEKMSEP
                     QHDRTYYPVEIAVPGAVDRIWAWTRQVRVSGNRQALRFVAMDPFDQVLLDQYVNRKPR
                     "
     gene            complement(2637..>3259)
                     /locus_tag="PK710_22910"
     CDS             complement(2637..>3259)
                     /locus_tag="PK710_22910"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT12650.1"
                     /translation="QDLHAILQTENVQGGTWLGWSFGAQVLLEAFRHPGPHPDRLILI
                     NPCYGRRPRDPAGIRRLLPFGLDAMAWSPEWWERAVRRASSWPETASWLKRLGFVAPA
                     ADQEGLLDILRHFRSVNAHAFLRLLRAAATHRIDGILGGIDVPTLVIFGDKDWVTPRF
                     NAEPLARQISNVELFLVRNGTHFVPLEYPELINLRVEKFLREHASL"
BASE COUNT          696 a         1057 c          874 g          632 t
ORIGIN      
        1 cgtgaaccac ccgcaaacca aaagcacgaa cggccttttc cagcccgtcg accaaccgat
       61 ccgacgccgt gcttcgaccc aacgtgccaa tcaccacatc cgcatcaacc ccctcggatg
      121 gcatggaatg catgctcaac accaccaccg tgccaaaccg ctgacaccta tgctcgacga
      181 cctcatcgag cgccccatga tatggcttgt agaaacaatc gagccgtcgc tgcaactctt
      241 gcggttccac cggctgccgt aacgcctgac atccggacct cgtttctcgc cacaataacc
      301 catgcggaca cgacgatgcc gtcgcccccc gcacgcttcg cccatccaca tcgtctggcg
      361 cccggttcag atccagcaca taacggctcc accgagacgc gataatcgtc gccccttctt
      421 cgaacagatc cgtcaccaat tcatccacgt acaagtccgc gtccgcagca agggacatga
      481 ccggcgccac gcaatgggcc atcgattccg gatccatgaa caaacccgca tgagggactt
      541 ccaccaccac gggggtatgc cccgtccgcg gctcgaacac ctcgaaaggc gcggaaatta
      601 ccatagaacg actctcccat cccagcacca tgcccctcgc tccccctttc atcgcgctcg
      661 aggatgcgtt tcgcgataca cccttcggac atgatcatcg gcgacatggg tgtaaatttc
      721 cgtggtgcca atatccgcat ggcccaacat ggcctggact gcccgaaggt ccgcaccgtg
      781 gcgcagcaaa tgagtcgcga aggaatgtcg aagtttatgg ggcgaaatgg gcttcatgat
      841 gcccaccccc cgagcataac gcaccacgag tttccaaaac ccttgccgcg tcaaggctcc
      901 cccccgcggc gatacgaaaa gcaccgtatc cttttcggtg gcatggtggg ctcgccccat
      961 ttccaaatag gtttggatgg catcgagcgc gacttcgccc acgggcacca atctcgattt
     1021 tccacctttt cctgtaactt tgacgattcc cgcgcgaaaa tccacggctc caagacgcaa
     1081 ccccacaagt tcactcaccc tcaatcccgc cgcatacatc aatcgcaaca tggcccgatc
     1141 ccgaattcct cgcggcgtgg actcgtccgg cgcatccaac agacgatcca cttcctgttc
     1201 gtcgagccat atcggaagct tgcgacgcgt gcgcggacga tcgatgagca cgcaagggtc
     1261 ggtggagatt gctttttccc gcaccaaaaa gcgacaaaac ccgcgaaccg ccgacaaatg
     1321 ccgggcggcg gagcgcgccc caagtccctg ttttcccagt tctaccaggt atacagacac
     1381 cagaggcgta tcgatatcct tgggtgtctt tgccccttgc tcctccgcca aggccagcaa
     1441 ttttcccaag tcccgagaat acgactccac cgtgttgcgg gaaagcgcac gttcgatccg
     1501 caaataatcg aggtaagcat cgacccaggc gtgaaggtcc acccgccgat cctaccgcaa
     1561 cgccccgtgc gccgtcgact ttcccgccat cggtccgccc gcgtacgcta ccccccgtag
     1621 tgattgccaa cttgccgatg taccgcttgg ctcaccgtcg aagaccgaaa ccacgtccgg
     1681 cagatcgaat ataacatatt gaatttgcat attttttatt gccccgtcga atctgggagt
     1741 atggcgcaag cgtcagcggt ctggggccca gcgcttaacg ttttctcaac ctcgagcgct
     1801 tgcttttcga gatcgcgttg cagctccatc tgctcgacga ccttcgtcaa ttgctcggat
     1861 cgaagggcgg tcacataggc cgtgagccgc tgccgacgct gttcggtcaa tgcgagcagg
     1921 tctttggccc gctctgctcc ccacacggtt tgcggtttcc aggcttttgc ggcagaaatc
     1981 gcttcgtcca tgggtatttt cgcgtcggcc tcgatccgat ccgccgcttg tcgcggacgt
     2041 ccttgcagag ctagctgatc cgcttcatgc gagatgcgcg caacccgctt gtctttttcc
     2101 aaaaccgcct gcgcctgccg acgatcctgt tcacacgaag ccattccacc gcggccgcat
     2161 ccgggcagca acccgagcat cagcagcaaa agagcgcggc taaccatcat cggggttttc
     2221 gattcacgta ttggtcgagc aatacctgat cgaagggatc catcgccacg aaacgcaagg
     2281 cctggcgatt gcccgagacg cgaacctgcc gcgtccatgc ccagattcgg tccacggccc
     2341 ccggaaccgc gatttccact ggataatacg ttcggtcatg ctgcggctca ctcatctttt
     2401 ccagcagcac cccccccttc gatatatcca tcactcgaca agcatacgga tacccgtcga
     2461 tatacttgtt gatcagcaga tcgacccggg ttcgtttcga gactcgtcgt tccatgggcg
     2521 aatacctcca cccacgaagt ataagcgaac cgaaacaatg tgatcaaatg tatgcacaag
     2581 tacagtgaca acccaaggga agatgctctt ggggcaagca ataaaacttt gtaaaattac
     2641 aagcttgcgt gttcccgcaa gaacttctcc acccgcaagt tgatgagttc cgggtattcg
     2701 agcggtacga aatgggtgcc atttcgcacg agaaatagct cgacgttgga gatttgtcgg
     2761 gcgagcggct cggcgttgaa ccgaggcgtg acccagtcct tgtcgccgaa gatgacgagg
     2821 gtggggacat cgattccgcc gaggataccg tcgatgcggt gggtggcggc ggcacggagc
     2881 aagcgcagaa aggcgtgggc gttgacgctg cggaagtggc gaaggatatc gagcaggcct
     2941 tcttggtcgg cggcgggagc gacgaaaccg aggcgtttca accaggaggc ggtttccggc
     3001 caggagctgg cacgacgcac ggcgcgctcc caccattcgg gggaccaggc catggcgtcg
     3061 aggccgaagg gcagcaggcg tcggataccg gcgggatctc gcgggcgacg gccgtagcag
     3121 gggttgatga ggatgagtcg gtcggggtgg gggccggggt gccggaaggc ttcgagcagg
     3181 acctgggcgc cgaagctcca tcccagccag gtgccgcctt gcacgttttc cgtttgcaag
     3241 atggcgtgca agtcctggg
//
LOCUS       DAOSOR010000638         3259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_29529_length_3259_cov_4.516230, whole genome shotgun sequence.
ACCESSION   DAOSOR010000638 DAOSOR010000000
VERSION     DAOSOR010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29529_length_3259_cov_4.516230"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="PK710_22915"
     CDS             <1..857
                     /locus_tag="PK710_22915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12651.1"
                     /translation="GGGETGGTGGGETGGTGGGETGGTGGGETGGTGGAAGQGGVAGS
                     AGTAGTAGTAGTAGTAGTAGTAGAAGTAGAAGSGGQSNCTLITYENLAPYQVAPGQGA
                     VFYAEVDPNIEGADPDLGSLEFYEEATGTFNLGTGDNANYETCSQCMRVLADVTQSAV
                     GKFFFQSKGMITVDASAGPTSGGLKATLTGVELIEVTIDENTAKSTPVPNGACLALPA
                     SFELYIEPPPPTWTCDLEYYRDGQFCDCTCGAWDPDCDDPANDVFDCDDGEVCKKDGS
                     GDPICTNP"
     gene            1093..1893
                     /locus_tag="PK710_22920"
     CDS             1093..1893
                     /locus_tag="PK710_22920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12652.1"
                     /translation="MASLPAGATACGPLRCLHFETPEDAFRFVLSFQPLILAIGESHA
                     KKDKAAVASAAKRFTDTLLPFLKDKASDLVVELMAPNPSCKKTTQQVKKSHEVVTQQQ
                     APTAQNEYVLMGERARTLGVVPDLLRPSCEDLSAIQRAGDDPIAVTLSTIARLTIAHV
                     EKLLRSASRKPEDADKMIVTYGGALHNDPSPSEDRALWSFGPDLSRISNGHYVALDLF
                     VPEYMDDTESWRALPWFAHYDADRMGEKTTLFHLDDGSFVLIFPRSDL"
     gene            complement(1909..2169)
                     /locus_tag="PK710_22925"
     CDS             complement(1909..2169)
                     /locus_tag="PK710_22925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12653.1"
                     /translation="MHRKDQMMQELNGLIRELLELRYRGGDYTRLAHAQGVVDGYMQA
                     LLDSGLASREELLALVRQQRAVIDGPATRSVYLDEIETTVAA"
     gene            2365..2763
                     /locus_tag="PK710_22930"
     CDS             2365..2763
                     /locus_tag="PK710_22930"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOT12654.1"
                     /translation="MRISIFKSKIHRATVTHADLDYEGSVTIDRDLLEAADIVPNEMV
                     HIWNVTRGTRLVTYALEGPAGSGAICVNGAAAHQNRPGDLVILATFAEMTREEARVYE
                     PRVVMVDQNNRIIDADAKEKPGPVLHSWMV"
     gene            2881..>3259
                     /locus_tag="PK710_22935"
     CDS             2881..>3259
                     /locus_tag="PK710_22935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12655.1"
                     /translation="MTSTASPMLKAIAAHPRANDLARFTRDCLLDSAPNLPDAPTVLE
                     RAAARGLSPDDAVVESTRVTDALVAPSRVEDSILLGTLLAYGLALDAVDTATQTQTRK
                     LVQLATHAGIDAWSSLDATMGEAA"
BASE COUNT          673 a         1007 c          908 g          671 t
ORIGIN      
        1 ccggtggtgg tgagacggga ggaaccggtg gtggtgagac gggaggaacc ggtggtggtg
       61 agacgggagg aaccggtggt ggtgagacgg gaggaaccgg tggagccgcc gggcaaggtg
      121 gagtcgcggg ctccgctggt accgctggta ccgctggtac cgctggtacc gcaggtaccg
      181 ctggtaccgc tggtaccgca ggtgccgctg gtaccgcagg tgccgcaggt tccggtgggc
      241 aaagcaactg cacgctcatt acgtatgaaa acctcgcgcc ctaccaagtt gctccagggc
      301 aaggcgccgt tttctacgcg gaagtagacc ccaacatcga aggtgccgat ccggatttgg
      361 gctcgctcga gttttatgaa gaagccacag ggacattcaa cctgggaacg ggagacaacg
      421 caaactacga aacatgctcg caatgcatgc gagttctcgc ggatgtcaca cagtctgcgg
      481 tgggcaagtt cttcttccag tccaaaggca tgataaccgt agatgccagc gcgggcccca
      541 cgagcggtgg actcaaagca actcttacgg gtgtggagtt gattgaggtc acgatcgatg
      601 aaaacaccgc caagtccact ccggttccga atggcgcttg cctcgccctg cccgcctcat
      661 tcgaactcta catcgagcct cctcctccga catggacctg cgacctcgag tactacaggg
      721 atgggcagtt ctgcgactgc acctgcggcg catgggaccc tgattgtgac gaccccgcca
      781 atgacgtttt tgactgcgat gatggggaag tctgcaagaa agacgggagt ggagacccga
      841 tttgcacaaa cccctgaatc atccctgaaa aacactgaca acgccgccct tccccttctt
      901 tcttgacaac gctcgacgtt cgcgctcacc actgtggtgg tgaatcgcta ttttcccatt
      961 ctccctgtcg ttctgctgat tgcggccgcg tgtgatcggg cgaccaccgt cccaccagca
     1021 cccatccctg ttgtcgagtc ttcttcctcc cctaccccgg ttgcgtcggc atctgcatct
     1081 gcatccgcgt ccatggcttc gctacctgcc ggagctaccg cttgcggccc gcttcgatgc
     1141 cttcacttcg agactccgga agatgcgttt cgcttcgtgc tctcctttca gcctctcatt
     1201 ctcgccatcg gagaatccca cgcgaaaaaa gacaaagcag ccgttgcctc ggcggccaaa
     1261 cggtttaccg acacgttgct tcccttcctc aaagacaaag cttcggatct ggtggtcgag
     1321 ttgatggcgc ccaacccctc atgcaaaaaa accacccagc aagtgaaaaa atcgcatgaa
     1381 gtcgtcacac aacaacaagc ccccaccgca caaaacgagt atgtcctcat gggagagcga
     1441 gcgcgaacgc tgggagtggt tcctgacttg ctccgcccct cgtgcgaaga cctcagcgcc
     1501 atacaacgcg ctggtgacga ccccatcgcg gtcacgctat cgacaattgc acgcctgacg
     1561 atcgctcatg tcgaaaaatt gctgcgttct gccagccgca agcccgaaga tgcggacaag
     1621 atgatcgtga cctacggagg ggctcttcac aacgatccgt cgccttccga agaccgggct
     1681 ttatggtctt ttgggcctga tctttcgcgt atttcgaacg gtcactacgt ggcgctcgac
     1741 cttttcgtac ccgagtatat ggatgacacg gaaagctggc gcgcgttgcc gtggttcgcg
     1801 cattacgatg cggaccgcat gggtgaaaaa acgaccttat tccatctcga tgatgggagt
     1861 ttcgtgctta ttttcccccg ttccgacctt tgatgccgat gccctcgatc atgcggcgac
     1921 ggtcgtctcg atctcgtcga gatacacgga gcgagtagca ggtccatcga tcacggcgcg
     1981 ttgttgtcgc acgagcgcca gcaactcttc ccgtgaggcc agaccgctat ccagcagcgc
     2041 ttgcatatag ccatcgacga ccccctgggc atgggccaga cgtgtgtaat ccccgccgcg
     2101 atagcgcaat tcaagcagtt cacgaatcaa tccgttgagt tcttgcatca tctggtcttt
     2161 gcggtgcatc acgcgtttcc tccgtaggga cccaagccac gtcgggttgt tttttgaacc
     2221 gctaccgaag gtgggagccg acggccaact ttcacggaac aagatcttga aacgcgcgag
     2281 atagctgcct aagttcgagc cgttgcgcag gtggacctgg gcctcccaag ccgccattca
     2341 gcaacccgga aagaagcttg tcgtatgcgt atcagcatat tcaagtccaa gattcacaga
     2401 gcgacggtga cccacgccga cctcgactat gaaggcagcg taacgatcga ccgagatctg
     2461 ttggaagccg cggatatcgt ccccaacgag atggttcata tctggaacgt cacgcgggga
     2521 acacgtttgg tgacctatgc tctcgaaggg cccgctggct ccggcgcgat ttgcgtcaac
     2581 ggtgccgcgg cgcatcaaaa tcggcccggg gacctggtca ttctcgccac gttcgcggag
     2641 atgactcggg aagaagctcg cgtttatgaa ccgcgggtgg tgatggtcga tcaaaacaac
     2701 cgcatcatcg acgcggacgc gaaagaaaag cccggacccg tgctccatag ttggatggtc
     2761 tgatcgccaa aagcaccggg cccacctttt catttcgcag aatactggcc tccaacgccg
     2821 ggttccttta tggcggcaag gtagagtgtt cgaattcgtt cctctggccc ggattgaacc
     2881 atgacgtcta ccgcttctcc gatgctgaag gctatcgccg cccatccccg tgccaatgat
     2941 ctggctcgct tcacacgcga ttgtctcctg gatagcgccc cgaatctccc cgacgcgccc
     3001 acggtgttgg aacgtgccgc ggcacgaggg ctgagccccg acgacgctgt cgtcgaatcc
     3061 actcgcgtaa ccgatgcgct cgtggctccc tctcgcgtcg aggattctat cctcctggga
     3121 actttgctcg cttacggcct cgccctggat gccgtcgaca ccgccacaca aacccagaca
     3181 cgcaaactcg tgcaactcgc aacccatgcc ggcatcgatg cttggtcctc gctcgacgcc
     3241 acgatgggcg aagccgccg
//
LOCUS       DAOSOR010000639         3257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_29553_length_3257_cov_4.568082, whole genome shotgun sequence.
ACCESSION   DAOSOR010000639 DAOSOR010000000
VERSION     DAOSOR010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29553_length_3257_cov_4.568082"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="PK710_22940"
     CDS             <1..854
                     /locus_tag="PK710_22940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12656.1"
                     /translation="YNRINLRGGGEFIWKPGGGMFDWRLGYEYGAVLFEEDQFDSLNN
                     ATHKIFTRGRWKFLPRTALLFDANTAFVRYPHVDANSYLLGSNPLRARVGFNGLMTDR
                     LALLAMVGWGASFHRTGSVPVENFDSVIGQAQLTFYPTLAPTDGSGQAEAAYTLSSVA
                     LGYVRDFENSYFGSFYGRDRGYLRASAFIVQRVVIEGEGGLSRVGFPNLYFADGTQRS
                     GAFHETRYDASFHTEYRVLPTVGINATVTYDKNDSVSLLTAAGSTIEDDLSNDRLQVF
                     VGARWFM"
     gene            929..1456
                     /locus_tag="PK710_22945"
     CDS             929..1456
                     /locus_tag="PK710_22945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOT12657.1"
                     /translation="MTNRVNLPPPVESTTIGPGDTFELFIVGEDKIPTAYTVSPDGTV
                     DFPYIHRQRVVGLEPQQVVDLVREQLQAQGILSDPSVMVNMKEYRSKFVNILGQVKSP
                     GTFPLVPGFTLVQAISKAGGPSPIADSDNVRLTRTTNGERRTLRVSLRSITEGRSPDI
                     PLQSGDVITIAERVF"
     gene            complement(1453..3129)
                     /locus_tag="PK710_22950"
     CDS             complement(1453..3129)
                     /locus_tag="PK710_22950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOT12658.1"
                     /translation="MKESAKQYENALKSWEEGPLAKAKAKARPRPEPGLLARSYDPPD
                     LFGPDDLEAIGFDPLRDLGMPGEPPYTRGVQPNMYRGRYWTMRQYAGFGTAKESNERY
                     RYLLAQGQTGLSVAFDLPTQMGIDSDSPRAAGEVGRVGVAIDSIEDMRVLLDGVPQDR
                     VSTSMTINSTAAVLLCLYIAVAEEKGIARNVLRGTIQNDILKEYIARGTYVYPPRPSL
                     RLISDIFAFCGAEVPKWNTISISGYHMREAGCDAAQEIAFTLANGLEYVKTAVSAGLD
                     VNAFGEQLSFFFNGHNNFLEEIAKFRAARRLWSTYMKERFGATSPRARALRFHCQTAG
                     ATLTAQQPHNNAVRVAIQALAAVLGGCQSLHTNSFDEALGLPTQQSATIALRTQQIIA
                     HETGVADFVDALAGSYAVESLTHTIEREAKAYIERIDELGGAVAAIEQGYIQREIQSS
                     AYQYQLDIEQKRRIIVGQNDFISDTEAVPILKVDPKEEQAQAQRLRAFRARRDETQWA
                     ASLQRIDTVARGSDNLVPPILEAVKVGATVGEISDALRGVWGEFTEVLTI"
BASE COUNT          678 a          851 c          920 g          808 t
ORIGIN      
        1 cgtacaaccg aatcaatctg cgcggaggag gggagttcat ttggaaacct gggggtggca
       61 tgttcgattg gcggttgggc tatgaatacg gtgccgtcct tttcgaagaa gaccaattcg
      121 attcgttgaa caacgcgacg cataagattt tcacgcgggg tcgctggaag tttttgccac
      181 gaaccgcgtt gctattcgat gccaatactg cgttcgttcg ctaccctcac gtcgatgcga
      241 acagttactt gctggggtcc aatccgttgc gagcgagagt tggcttcaac gggctcatga
      301 ccgatcggtt agctttgctt gcgatggtcg gctggggagc cagtttccat cggaccgggt
      361 cggttcctgt ggaaaacttc gatagcgtca tcggccaagc gcaacttact ttctacccca
      421 cgcttgcgcc caccgatgga agcggacaag cagaagccgc ttatacccta tcgtcggtgg
      481 ccttgggata tgtgcgagac ttcgaaaaca gctactttgg aagcttttat ggacgcgatc
      541 gcggctatct tcgagcatct gctttcattg ttcaacgcgt agtcatcgaa ggggaaggag
      601 gcctttcccg agttggtttc cccaacttat acttcgccga tggcacacag cgttcggggg
      661 cttttcatga aacacgctac gatgcttcct tccataccga atatcgcgtt ttgccaaccg
      721 tcggcatcaa tgcaaccgta acctatgaca aaaacgatag cgtttcgctg ctgacggccg
      781 cgggatcaac gatcgaagac gatctcagta atgatcgttt gcaagtgttt gtgggtgctc
      841 ggtggttcat gtagccgaaa tcaattctgc acgaaggctc gtgtcgtttg gattgtgcgc
      901 gcttttcgtc agttcatgcg ccgcgcccat gaccaatcga gtcaatctcc cgcctcccgt
      961 ggaaagcaca accataggac cgggggatac cttcgagcta ttcatcgtcg gggaagacaa
     1021 aatccccacc gcctatactg tctctcccga cggtacggtg gacttccctt atattcatcg
     1081 tcaacgggtg gtaggtctcg agcctcagca ggttgtcgat ctggttcgag aacaactcca
     1141 agcgcagggc attctttccg atccatcggt gatggtgaac atgaaagaat accgttcgaa
     1201 gttcgtgaac atccttggtc aggtcaagtc tccgggaacc ttccccttgg ttcccggttt
     1261 cacgttggtc caggcgattt cgaaagcggg cggtccgagt cccatcgcgg attccgacaa
     1321 tgttcgtctc acacgaacga ccaacggaga gcgtcggacg ctgcgggttt cgttgcgttc
     1381 catcacggag gggagaagtc cggatatacc gcttcaatcg ggtgatgtga ttacgatcgc
     1441 ggagcgcgtg ttttagatcg tcagcacttc ggtgaattcg ccccatacgc cacgaagggc
     1501 atcgctgatt tccccaaccg tggcgcctac tttcaccgct tccagaatgg ggggcaccaa
     1561 gttgtcgctt cctcgtgcga ccgtatcgat gcgttgaagc gacgcggccc attgggtttc
     1621 gtcgcgtcgc gctcgaaacg ctcgaagtcg ttgggcttgt gcttgttctt ctttgggatc
     1681 gactttgagg atgggaaccg cttccgtatc gctgatgaaa tcgttttggc ctacgatgat
     1741 ccttcgtttc tgttcgatat cgagttggta ttgataggcg gagctttgga tttctcgctg
     1801 gatataccct tgttcgatgg ccgcgaccgc tcctcccagc tcgtcgattc gttcaatata
     1861 tgctttggct tctctttcta tcgtatgtgt gagcgattcc acggcgtaac ttccggccag
     1921 ggcatcgacg aaatcggcaa cgccagtttc atgagcgatg atctgctgtg ttcgcaaggc
     1981 gatggtggca ctctgttggg tgggcaggcc aagggcttca tcgaagctat tggtgtgcag
     2041 ggattggcaa cctccaagca cagcggcgag agcttgaatc gccacgcgca cggcattgtt
     2101 gtgcggttgt tgtgcggtca gggttgcccc cgctgtttga caatgaaatc gcagcgcgcg
     2161 ggcacgcggg gaggtagccc cgaatcgttc cttcatatac gtggaccata aacgtcgggc
     2221 tgcacggaat tttgcgattt cctccaagaa attattgtgc ccattgaaga aaaagctgag
     2281 ttgttctccg aaggcattga catcgagccc agcggaaaca gcggttttca catattctag
     2341 tccgtttgcg agagtgaaag cgatctcctg agcggcatcg caacctgctt ctcgcatgtg
     2401 gtaaccactg atcgaaatcg tattccattt gggtacttcg gccccacaaa acgcaaaaat
     2461 atccgaaatc aatcgcagcg atggtcgagg aggatatacg tacgtcccgc gcgcgatgta
     2521 ctctttgagg atatcgttct ggatcgtgcc tcggaggaca tttcttgcga tgcctttctc
     2581 ctcggcgaca gcgatataaa ggcagagcag cacggcagcg gtggagttga tggtcatcga
     2641 tgtggagacg cggtcttggg ggacgccatc gagcaacacg cgcatgtctt cgatggagtc
     2701 gatggcgacg ccgacgcgtc cgacctctcc agcagcacga ggggagtccg aatcgatgcc
     2761 catttgggta ggcagatcga aagccacgct gagtccggtt tgtccttgtg cgagcagata
     2821 tcgataccgt tcgttggatt ccttggcggt cccgaatcca gcgtattgtc gcatggtcca
     2881 ataacgaccc cggtacatat tgggttgaac gcctcgggta taaggaggct caccgggcat
     2941 tcccagatct cgcaaaggat cgaaaccgat ggcttccaaa tcatcggggc cgaatagatc
     3001 gggtgggtca taagagcgag cgagaaggcc tggttccggt cgagggcgag ccttggcttt
     3061 ggctttggcc aaaggacctt cttcccagga tttgagagcg ttttcgtact gctttgcgct
     3121 ttccttcata cctcacctgt ctttccattt ttccgaacga agaacacgaa gcaacgcgga
     3181 agaggtggca tgagccactt gttccgccgt tcgtgttgcc atgagcgagt cgaacaattc
     3241 catcgcaacg atgagaa
//
LOCUS       DAOSOR010000640         3253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_29591_length_3253_cov_3.927455, whole genome shotgun sequence.
ACCESSION   DAOSOR010000640 DAOSOR010000000
VERSION     DAOSOR010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29591_length_3253_cov_3.927455"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PK710_22955"
     CDS             complement(<1..275)
                     /locus_tag="PK710_22955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12659.1"
                     /translation="MKAVSALVAVIVLFAIAYMVGPSAKFVFGVAVPYAAMAVFLGGI
                     IYRVAFKWGKAPVPFAIATTCGQQKSLPWIKHASLENPSTTGGVILR"
     gene            complement(272..805)
                     /locus_tag="PK710_22960"
     CDS             complement(272..805)
                     /locus_tag="PK710_22960"
                     /inference="COORDINATES: protein motif:HMM:NF025719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsbRD N-terminal domain-containing protein"
                     /protein_id="HOT12660.1"
                     /translation="MLTHKRATLTQRWTDAVLNTYPADSARFMRRQKDAFANPGGHLV
                     RQCIEALVDALEQGVEVAKVDAALEGLLKLRALQDFTPAQAVQFVFIAKTILREELSL
                     PAGDPSWERWDTRIDALALVAFDVYMRCREKVYEVRYNQLKKLNHVMLERANRPATHA
                     RSNGSSEWEPNDRGDEG"
     gene            complement(1104..1421)
                     /locus_tag="PK710_22965"
     CDS             complement(1104..1421)
                     /locus_tag="PK710_22965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HOT12661.1"
                     /translation="MATFEYEGVSIQVDEDGFMEEPEKWNEEVAKALASTEGVTELTE
                     DHWKVVNYLRDYYLKFGVAPMIRKLCKESKYSLKQIYELFPSGPAKGACKVAGLPKPT
                     GCV"
     gene            complement(1476..3164)
                     /locus_tag="PK710_22970"
     CDS             complement(1476..3164)
                     /locus_tag="PK710_22970"
                     /inference="COORDINATES: protein motif:HMM:NF009410.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOT12662.1"
                     /translation="MALVLKQKKKLQTRAVGSGGPRGGGETSALRPKHVLKTPPCTDG
                     CPIHNDIRGALQIMAQTEDYGRTREESLELAWERFTETSPFPAVCGRVCPHPCETVCN
                     RADMEGAVSINQLERIIGDFGLQKGLALKKLTDEVHPEKIAVIGAGPAGLSCAYQLAR
                     RGYRVTVFEAFSQTGGMLRYGIPRYRLPADVLDGEIARIEKLGVEIKLNTAVGRDFPY
                     TDLRKDYAAIFVGIGAHKGKALRIPGEDAANVWTATTFLNAVMSGKQVDVGNKVLVIG
                     GGDAAIDAARVSRRLGAEVWIVYRRTIHEMPAIEPEIEGAKEEGVNFEFLVMPLEVIK
                     EGDRAVAMKFQRTELGEPDASGRRRPVPIEGAVFTLDCTTVVSAISQEPDFEGFDDLH
                     TGRDWVKVDEFHRTNQEGVYAGGDVLDLALVTTAIAQGSRAAATIHQVLRCEQVSITP
                     MAPIIHKDKMNRAWFEKKDRVVPQPGADVPTRLAQPELEVEKGLTQDEAFEEAKRCMS
                     CGLCFECGHCWTMCGDGAIIRPLNKGEPYAFKLEFCQGCKKCAEQCPCGYIEMA"
BASE COUNT          697 a          988 c          864 g          704 t
ORIGIN      
        1 atgcgcagaa tcactccgcc tgtcgtggac ggattttcga gggaggcatg tttgatccac
       61 ggcagcgatt tttgctggcc acaggtggtg gcgatggcaa agggtacggg tgccttgccc
      121 cacttgaaag caacccgata gatgatgccg ccaaggaaaa ccgccatggc cgcgtagggc
      181 acggcaacac caaagacaaa cttcgcgctc ggtccgacca tgtacgcgat ggcgaacagc
      241 acgatcacag ccacgagggc ggataccgct ttcatccttc gtcacccctg tcgttgggtt
      301 cccattcgga agaaccgttg gatcgagcgt gcgtcgcagg acgatttgcg cgttccaaca
      361 tcacgtggtt cagcttcttg agttggttgt aacggacttc atacactttc tcgcgacagc
      421 gcatgtacac atcgaaggca acgagcgcca aagcatcgat gcgcgtgtcc catcgttccc
      481 aggaagggtc gcccgccggc aagctcagtt cttctcgtaa aatggtcttg gcgatgaaca
      541 cgaattgaac ggcctgcgca ggcgtgaagt cctgcaacgc ccgcagtttc aataggccct
      601 ccaaagccgc atcgaccttg gccacttcca caccttgctc gagcgcgtcc acgagcgcct
      661 cgatgcattg acgcacgagg tggcccccag gattggcaaa agcatccttt tgacgtcgca
      721 tgaacctggc gctgtcagcc ggatacgtat tgagcacggc atcggtccag cgctgcgtga
      781 gcgtggcgcg cttgtgcgtg agcaacggct cctccatggt agacgtccgg gggcacgaaa
      841 ggtgaggccc acgggtcaat cacccgtata tgcaaggctt gattgcaggt caaccatgca
      901 agcgagaggg actgagattg gtcacaacgt catagtgctg acaaatgacg taagcggtag
      961 agtatttaac tcacaatata ttcgttagca taggctacga cacagtcaat gagcgggcat
     1021 ccatgatgag gggataaggt gtagcccaac gaaagaaggg atttatcgtt gggcgtgaga
     1081 agggaaggtc tattgggacg acatcaaacg cagccggttg gcttgggcag tccggcgact
     1141 ttgcaggctc cctttgccgg gccgctcggg aaaagctcgt agatttgctt gagggagtac
     1201 ttggactcct tgcagagctt gcggatcatc ggagccacac cgaatttgag gtagtaatcc
     1261 cgaaggtagt tcacgacttt ccaatggtct tcggtcaatt ccgtgactcc ttcggtagaa
     1321 gcgagcgcct tcgctacttc ctcgttccac ttctcgggct cttccatgaa gccgtcttcg
     1381 tcgacttgaa tgctcacacc ctcgtactca aaggttgcca tgacgtgttt tctcctccac
     1441 tagcagcttc gttgttgcga aacaggtcct tacgattacg ccatttcgat ataaccgcag
     1501 gggcattgct ccgcgcactt cttgcagccc tggcagaact ctagcttgaa agcatagggc
     1561 tcgcccttgt tcaacggacg gataatggca ccatctccgc acatcgtcca gcagtgtccg
     1621 cactcgaaac acaaaccgca actcatgcag cgtttggcct cttcaaaagc ctcatcttgt
     1681 gtcaatccct tttcgacctc gagttcgggt tgcgcgaggc gcgtggggac atccgcgccg
     1741 ggctgaggca ccacacggtc ttttttctca aaccaggccc gattcatctt gtctttgtga
     1801 atgatcggtg ccatcggcgt gatcgacacc tgctcacaac gcaacacttg gtgaatcgtt
     1861 gccgcggcgc gactcccttg cgctatggcg gtcgtcacca gcgccaggtc cagaacgtcg
     1921 cctcccgcgt aaaccccctc ttgattggtc cggtggaact catcgacctt cacccaatcc
     1981 cggcccgtat gcaaatcatc gaatccttcg aagtccggct cctgactgat ggccgaaacc
     2041 acggtggtgc aatctagcgt aaacaccgcg ccttcgatgg gtacggggcg acgacgtccg
     2101 ctggcatcgg gctctcccaa ttcggttcgt tggaacttca tcgcgacagc acggtcgcct
     2161 tctttgatga cttccaacgg catgacgaga aactcgaagt tcaccccttc ctctttggct
     2221 ccctcgattt ccggctcgat cgcgggcatt tcgtgaatgg tgcgacgata cacgatccag
     2281 acttccgcgc cgagacgccg ggatacccgc gcggcatcga tggcggcatc accaccacca
     2341 atgacaagca ccttgttgcc cacgtcgacc tgcttgccag acatcaccgc attcaaaaag
     2401 gtcgtggccg tccatacgtt ggccgcatcc tcaccaggaa tgcgcaacgc cttaccctta
     2461 tgcgcaccaa ttcccacgaa aatggcagca tagtcttttc gcagatccgt ataggggaag
     2521 tcacgcccca cagccgtgtt gagcttaatc tccaccccca acttttcgat acgcgcaatc
     2581 tcaccgtcga gcacgtccgc aggcaatcga tagcgcggga taccataacg caacatccca
     2641 ccggtctggc tgaaagcctc gaataccgtc acccgatacc cgcgtcgcgc caattggtag
     2701 gcgcaggaaa ggcccgccgg acccgcacca atcacggcaa tcttctcggg atggacctcg
     2761 tcggtcaact tcttgagggc aagccccttc tgcaaaccga agtcaccgat gatacgctcc
     2821 aattggttga tggaaaccgc cccttccatg tctgcacgat tgcaaaccgt ctcgcaggga
     2881 tgcggacaga cacgcccaca gaccgccggg aatggagagg tttcggtaaa acgttcccag
     2941 gcaagctcca acgactcttc ccgcgttcga ccataatcct cggtctgcgc catgatttgc
     3001 aaggcgccgc gaatgtcgtt gtgaatggga cacccatcgg tgcatggtgg agtcttgagt
     3061 acgtgcttcg ggcggagcgc cgatgtctcg ccgcctcccc gtggccctcc cgaccctacc
     3121 gccctagtct gcaacttctt tttttgcttg agtacgagcg ccatcaaact ctcctaccga
     3181 ccccctagag tgttgtaggg gaccagtctt atagtgtcaa ggacttcacc ggaaggattc
     3241 catcgttgca tgg
//
LOCUS       DAOSOR010000641         3241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_29736_length_3241_cov_4.404896, whole genome shotgun sequence.
ACCESSION   DAOSOR010000641 DAOSOR010000000
VERSION     DAOSOR010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29736_length_3241_cov_4.404896"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="PK710_22975"
     CDS             <1..1317
                     /locus_tag="PK710_22975"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HOT12663.1"
                     /translation="GLPQDQVAVLFMSSDPSSQNGGMSLVCPVPAANPWATAVHGTGK
                     GQAWHITTDAPVSAYDIHPYGGAASFLPSAEMLLPTSAWGTNYVAVVPKLGEMLNPPS
                     GPGPQYGTIVAKENNTTVTVLPRVALPGGNGVAAAPANQPTQFTLQQHEFIQWEPSQE
                     MSGTIISSDKPVAFTGGNGYLCLKGMSSKGGGCDSGHQAIPPVAALGYRYVGIPYTTR
                     RADLQPESIIYRIVGAVAGTSLTFDPPVPGAPTTLDVGQMVDFEATGPFVVSSQDEDH
                     PFYLGQMMTGCFVHGGSRPGINPASLSAEYNCLGDEEYVNILPPAQFLSRYIFFTDPT
                     YGTTNLVLTRVKTPSGFKEVSVGCSGVVSGWQPVGSSGEFEIANVDLVRSAHGVAGCT
                     NGPQSAQSDGPFGLMVWGLDSFASYAYPAGGNIAPINTVVVPAVPK"
     gene            complement(1359..2981)
                     /locus_tag="PK710_22980"
     CDS             complement(1359..2981)
                     /locus_tag="PK710_22980"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT12664.1"
                     /translation="MSRRKRQAKSAAPAPCSPSEPPRAKLGERHALVGFFLLTVVLRW
                     IYFATADGPSFADPLIDGDYYDYLADRLSRGQGFEPGPFWQPPLYPIVLGLLYSVLGH
                     DLLWPRIMQSLADGLTAVLAVQIASRVLGNARWALAAGVLVALHGTLVFYSGEILPTQ
                     LAILASTVAVWQATRSSRTMKTAIGSGIAVGLGALAVATTMVLLLPLTWFWMKENQKQ
                     GLGLLAAGLVVVGTATAANVFRSDQWVLISANGGVNLYLGNAERANELQAIRPGGAWE
                     ALVNEPVDAGITSPSGQDGYFVRKALGWCASSPASCLGGLAWKARLLLRSQEIPRNES
                     VEVVRHQSPVSSVLLARVGSFALPHALLLPLAAAGIVYALRKKQAAATLVVWCTLALG
                     AMPVLFFVTGRYRTAMAAMLCVLAVLGAHGLWTLKKEAWREGLAAFVVLFAAVWPAPL
                     PVDAVSYEAEMHYAVGGRRARLGDDPQAATHWQRAVALRPDYLEAHFNLGLLYVRHQQ
                     WSKAIDAFLLVLSIDPDHAMARELIEECKKYQ"
BASE COUNT          718 a         1002 c          911 g          610 t
ORIGIN      
        1 gggttacccc aagaccaggt cgccgtactg ttcatgtcct cggatccatc ttcgcaaaac
       61 ggtggtatgt ccctggtttg ccccgtgcct gccgcgaacc cgtgggccac cgcagtccac
      121 ggaaccggaa aagggcaagc ctggcatatc accaccgatg cccccgtcag cgcctacgat
      181 atccacccct acggcggcgc ggcttctttt ctcccaagcg ccgaaatgct tctcccgaca
      241 agcgcctggg gaaccaacta tgtcgctgtc gttcccaaat taggtgagat gctcaatccg
      301 cccagcggac caggccccca atacggaacc atcgtcgcca aagaaaacaa caccaccgtg
      361 accgtgcttc ccagggtggc cttgccggga ggaaacggag tcgccgcggc tcccgccaac
      421 caacccaccc aattcacact gcaacaacat gaattcattc agtgggagcc ctctcaggaa
      481 atgagcggaa cgatcatttc ttccgataaa cccgtcgctt ttaccggagg aaacggctac
      541 ctctgcctca aaggcatgag ttccaaaggc ggaggatgtg attccggcca tcaagccatt
      601 ccacccgtcg ccgccctcgg ataccgctac gtaggaattc cttacaccac ccgacgcgct
      661 gatttgcagc cagaatccat catttatcga atcgtcggcg ccgtggcggg tacatccctc
      721 actttcgacc cacccgtgcc aggggccccc accaccctcg atgtgggaca aatggtggat
      781 ttcgaagcca cgggaccttt cgtcgtcagc agccaggatg aagaccaccc cttttacctc
      841 ggccaaatga tgacaggctg cttcgtgcat ggcggaagcc gccccggcat caatccggcc
      901 tctctgtcgg ccgagtacaa ttgcctgggc gacgaagaat acgtcaacat cctgcccccc
      961 gcccaattcc tctctcgcta tatcttcttc accgacccca cctacggcac caccaatctc
     1021 gtcttgaccc gcgtcaaaac cccgtccgga ttcaaagagg tgagcgtggg atgctcgggt
     1081 gtggtgtctg gctggcagcc cgtcggctca tcgggtgaat tcgaaatcgc aaacgtggac
     1141 ctggtgcgca gtgcccacgg agtggccggt tgtaccaacg gcccccaatc cgcgcaaagc
     1201 gatggcccct ttggactcat ggtgtggggt ctggattcct tcgcctccta cgcctacccc
     1261 gcgggaggca atatcgctcc catcaatacg gtcgtcgtcc ccgccgtgcc caaataggcg
     1321 gaggccccac ggagccactg caacataacc aattgtattt attgatattt tttacattcc
     1381 tcgatcagct ctcgcgccat ggcatggtcc ggatcgatgg aaagcaccaa caggaaggcg
     1441 tcgatggcct tcgaccattg ctgatggcgc acgtaaagca atcctaaatt gaagtgggct
     1501 tccaggtaat ccggacgcaa agccacggcg cgctgccaat gggtggctgc ctgcgggtcg
     1561 tcgccgagcc tggcgcgacg tccaccgacg gcataatgca tttctgcttc gtaggagacg
     1621 gcatcgacgg gcaagggagc gggccagaca gcggcaaaca ggaccacgaa cgcggcaagc
     1681 ccctctcgcc acgcctcttt tttcaaagtc cacaagccat gggcgccgag gacggccaac
     1741 acgcatagca tcgcggccat ggcggttcgg tagcggcccg tgacaaaaaa cagaacgggc
     1801 atggctccga gagcgagggt acaccagacg acaagggttg ctgccgcttg tttttttcga
     1861 agggcgtaga cgatgccagc ggcggccaaa gggagcaaaa gggcgtgagg caaggcaaaa
     1921 gaaccgacac gggcgagcaa gacggaggaa acgggggatt gatgacgaac gacctcgacg
     1981 ctttcgttgc gtgggatttc ctgggatctg agcaatagac gcgctttcca ggccaatccg
     2041 ccgaggcagg atgcggggct cgaggcacac cagcccaggg ctttgcgaac gaagtatccg
     2101 tcttgtcccg aggggcttgt gattccggca tcgacgggtt cattcaccag ggcctcccag
     2161 gcgccgcccg gccgaatggc ttgcaactca ttggcgcgct ccgcattgcc gagatagagg
     2221 ttgactcccc cgttggcgga aatgaggacc cattggtcgg agcgaaaaac gttcgcagcc
     2281 gtggccgtcc ccaccacgac aagtcccgcg gccaatagac caagcccttg cttttggttt
     2341 tccttcatcc aaaaccaggt caaaggaagt agcaacacca tggtcgtggc gaccgcaagc
     2401 gcccccaacc cgacggcaat tccgcttcct attgccgtct tcatggttcg cgaggacctg
     2461 gtggcctgcc agaccgcgac ggtagaggcc aagatggcga gctgggtggg aaggatctcc
     2521 ccgctataaa aaaccagggt tccgtgcagg gccaccagca cccccgcggc cagggcccat
     2581 cgtgcgtttc caagcaccct cgaggcgatc tgcacggcga gcacagcggt caggccatcc
     2641 gccagcgatt gcatgatccg aggccataga aggtcgtgcc caagcacgga atacaaaagt
     2701 ccgagcacga tggggtacaa ggggggttgc cagaacggcc ccggctcgaa cccttgccct
     2761 ctcgaaagcc ggtctgccag ataatcgtaa taatcgccgt cgatgagcgg gtccgcaaag
     2821 gagggcccat cggccgtggc gaaatatatc catcgcaaga ccacggtaag aaggaaaaac
     2881 cccacaagcg cgtggcgctc ccctagtttt gcccgcggtg gttcggaggg gctgcaaggg
     2941 gcaggtgccg cggatttggc ttggcgtttt cggcgactca cggcgttcac gtcacagagt
     3001 tggaaccgga attccgtttg gatttcatta atgaaacatc aatgaaattg tgttatgtta
     3061 actagctgaa aatacgacac tttctgaggc atagggtttg cttggtagag attcacgtat
     3121 ttttgcattt cgaaccgaaa tgagcaatca acgttcaacc ctacctcctg ctctcaatga
     3181 actcgacgcg ctgctattgg agtatgtgac ggagggggtc tcggtcgttc gaaacgcatc
     3241 g
//
LOCUS       DAOSOR010000642         3239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_29757_length_3239_cov_5.017274, whole genome shotgun sequence.
ACCESSION   DAOSOR010000642 DAOSOR010000000
VERSION     DAOSOR010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29757_length_3239_cov_5.017274"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="PK710_22985"
     CDS             complement(<1..1290)
                     /locus_tag="PK710_22985"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOT12665.1"
                     /translation="MTQHDPPSPNDTPSPGTGEPDAVPSAAPPSPSAAQPHRFRSPKE
                     RRESRKKRKITRTSLDTGLESLLGVIIVGSVLAVGTVHTYTLLLVGLLSTLAGTLALF
                     LYRKDAEEWPLTLPAGGILLLTLWTALQAIPLPASLVAILAPHNAETWRHALDPLGLP
                     GPAWHPISLDPGATWVEVLKGITYLGVLLTSTVVAFRRGATFGVGLVFVSALAAGAFT
                     IAHGIAGMTKVFGLYEPIYFSSPWQMGPLLNSNHLAGYLNLGVTCGLGTLLVRKPNVP
                     RWLSGLGVATLIALAVLAASRGATVLLPLGVALVVLLLRSRNIPEQEAVSRRWLNILT
                     FGAVAGGVTFTALGLTSRHWKALLDQDLGKLSILSWARPLVFDHPWLGIGRGAFETVY
                     PAYRPIQGHVLWTHPENLLVQWAAEWGLPVTLLTVGFF"
     gene            complement(1483..2736)
                     /gene="rho"
                     /locus_tag="PK710_22990"
     CDS             complement(1483..2736)
                     /gene="rho"
                     /locus_tag="PK710_22990"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOT12666.1"
                     /translation="MHLRELKQKPMVELVQLAKELNIEGGAGLRKQELIFALLQSQAA
                     KNGSIFGEGVLECLPDGFGFLRAPDYNYLPGPDDVYVSPSQIRRFGLRTGDSVSGTVR
                     PPKESERYFALLKVEKINGEAPEVARDKILFDNLTPLYPTAKFDLEILDGPRNNSTRI
                     VDLLVPIGKGQRCLIVSPPRAGKTMLLQDIANSIHRNHPDTVLIVLLIDERPEEVTDM
                     QRTVHGEVISSTFDEPATRHVQVAEMVIEKAKRLVEHKKDVVIMLDSITRLARAYNTV
                     VPPSGKILSGGVDSNALHKPKRFFGAARNLEEGGSLTIIATALVDTGSRMDEVIFEEF
                     KGTGNSEIHLDRKLMEKRIFPCLDINRSATRKEELLLPDWKLQRVWLLRSLLHPLNVI
                     DSMEFLLDKISRTQNNQEFLESMNQ"
     gene            complement(3023..>3239)
                     /locus_tag="PK710_22995"
     CDS             complement(3023..>3239)
                     /locus_tag="PK710_22995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12667.1"
                     /translation="VYSGNIRDIDGQSTVCGACATRIIERDGYRILSYRIDAQGRCEV
                     CGANVAGRFDERAGAWGEKRQRLRLGR"
BASE COUNT          657 a          774 c         1134 g          674 t
ORIGIN      
        1 gaaaaagccg acggtgagga gggtgacggg cagtccccat tccgcggccc attggacgag
       61 gagattttcg ggatgtgtcc aaagcacgtg gccttggatg ggccggtagg ctgggtacac
      121 ggtttcgaag gcgccacggc cgatgccaag ccaggggtgg tcgaagacga ggggacgcgc
      181 ccaggaaagg atggatagtt ttcccaggtc ttgatcgagc aacgctttcc aatggcggga
      241 ggtgaggccg agagcggtga acgtgacgcc tcctgccacg gcgccgaagg tgaggatgtt
      301 gagccatcgg cgggagacgg cttcttgttc ggggatattt ctggaacgaa gcaataacac
      361 gacgagagcg acgcccaggg ggagcaatac ggtagcgcct cgcgaggcgg ccaagacggc
      421 caaggcgatg agggtggcca cccccaatcc tgataaccat cgaggaacat taggttttct
      481 tacgagcagg gtgccgagtc cgcaggtgac gccgaggttg agatagccgg cgaggtggtt
      541 ggagttgagc aaaggcccca tctgccaggg ggatgaaaag tagatgggtt cgtagagccc
      601 aaagaccttg gtcattcccg cgataccgtg ggcaatggtg aaggcgccgg cggccagggc
      661 gctgacgaaa accaagccga cgccgaaggt ggcgccgcgt cgaaaagcga cgacggtgct
      721 tgtgagcagc acgccgaggt aggttattcc tttgaggacc tccacccagg ttgcgccggg
      781 gtcgagggag atgggatgcc aagccgggcc tggtagcccg agggggtcga gggcatgtcg
      841 ccaggtttcc gcgttatgag gggcgaggat ggcgaccagg cttgcgggca aagggatggc
      901 ctgcagcgcg gtccacagcg tcagcagcag gattccgccc gcgggcaagg tcaacggcca
      961 ttcctcggca tctttgcggt aaaggaatag ggcgagcgtt ccggcgagcg tgctcagaag
     1021 accgaccaaa agaagtgtgt aggtatgcac ggtgccgaca gcgagcacgc ttccgacgat
     1081 gatcacaccg agcaagcttt ctaggccggt atcgagggaa gtgcgcgtaa tctttcgctt
     1141 ttttctgctt tcccgacgct ctttggggga gcggaagcga tggggttgcg cggcggaagg
     1201 tgagggcgga gcggcggaag gaacggcgtc cggttctcca gtgccgggag agggggtgtc
     1261 gttgggggaa ggggggtcgt gttgggtcaa ggccagtgga tgagcgatgt tcggacaatt
     1321 atccgcggca agtcaatgac ggaggcgatg ggcatgaggg aattttggct ccgtgggcgg
     1381 atattcggtg atgcttgcgt gtgatgctcc tggcgcgggc cgggttcatg tggggaaggg
     1441 gaaaaacgaa ttggatgggg tggggggacg agggaggcgg gactattggt tcatcgactc
     1501 caggaactct tggttgtttt gcgtgcgaga gattttgtcg aggaggaatt ccatcgagtc
     1561 gatgacattg agaggatgca gcaagcttcg gagcagccag acgcgttgca gtttccaatc
     1621 gggcagaagg agttcttctt tgcgggtggc gctgcgattg atgtcgaggc aggggaagat
     1681 gcgtttttcc atcagtttgc gatcgagatg gatttcgctg ttcccggttc ctttgaactc
     1741 ttcgaagatg acttcgtcca tacgggagcc ggtatcgacc aaggcggtgg cgatgatggt
     1801 caacgagccg ccttcttcga ggtttcgcgc ggcgccgaag aagcgcttgg gtttgtgcaa
     1861 ggcgttggaa tccacgccgc cggagaggat ttttccgctg ggggggacga cggtattgta
     1921 ggcgcgggcc aagcgcgtga tggagtcgag catgatgacg acgtcttttt tgtgttcgac
     1981 gagtcgtttt gctttttcga tgaccatttc cgcgacctgg acgtggcggg tggcgggttc
     2041 atcgaaggtg gaggagatga cctcaccgtg gacggtgcgt tgcatatcgg tgacttcttc
     2101 gggtcgttca tcgatgagca agacgatgag gacggtatcg gggtgattgc gatggatgga
     2161 gttggcgata tcttgcagca gcatggtctt accggcgcga ggcggtgaga cgatgaggca
     2221 gcgttgacct tttccgatgg gcacgagcaa gtcgacgatt cgcgtggagt tattgcgtgg
     2281 tccatcgagg atttcgaggt cgaacttggc ggtaggatac aagggggtga ggttgtcgaa
     2341 gaggattttg tcgcgggcga cttcgggggc ctcgccattg attttttcga ctttgagcaa
     2401 agcgaagtag cgttcgcttt ccttgggggg gcggacggtg cccgagacgc tatctccggt
     2461 gcgcaagccg aagcgtcgga tttgggaagg ggagacgtaa acgtcgtcgg gtccgggaag
     2521 gtaattatag tcgggcgcac gcaagaagcc gaagccatcg ggcaggcatt cgagaacgcc
     2581 ttccccgaag atacttccat ttttcgccgc ctggctttgc agaagcgcga aaataagctc
     2641 ttgtttacgg aggccggcgc ctccttcgat attgagctct ttggcgagct gaacaagctc
     2701 taccatcggc ttctgcttca actctcgaag atgcatgggc tgagtgtcct ccacccccac
     2761 gttgtcacgc gctgattcat cacattcggt ctggcgtgtg aacgatccgt gagggttgtt
     2821 gtcgaggggc acggctcaaa acgtttatgg aattcagagt ccccgcggga gacaagccgc
     2881 gccgttgaga tgtcgttgaa gaaaaacggg gacgtgttcg cggactacga acacgtttct
     2941 cctatcgatc aagccgggcg tcctgtcaat ctgagaaacg atcgaaatcg tcttcggcga
     3001 atcgtaggtg acgaatcgag ggtcatcttc ccaaccgaag gcgttggcgt ttttcgcccc
     3061 acgcgcctgc ccgctcatcg aaacgtcctg ccacgtttgc gccgcaaacc tcgcatcgac
     3121 cctgtgcatc gatccgatac gacaaaatcc gatatccatc acgttcgatg atgcgcgtgg
     3181 cacacgctcc gcaaacggtg gattgaccgt cgatatcgcg aatattgccg gaatagacg
//
LOCUS       DAOSOR010000643         3197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_30223_length_3197_cov_3.630490, whole genome shotgun sequence.
ACCESSION   DAOSOR010000643 DAOSOR010000000
VERSION     DAOSOR010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30223_length_3197_cov_3.630490"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1415)
                     /locus_tag="PK710_23000"
     CDS             complement(150..1415)
                     /locus_tag="PK710_23000"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT12668.1"
                     /translation="MRGLLAGLARWWRLRTLWENARMALSSVRANALRSLLTCLGIVI
                     GVATVIGILSIVGGINNLVTGELDRVGASSFFLQKYPGVRVGFMREYRLRPNLTSDDA
                     KAILLACPSVESVSPSVRFRSQQVRSGNNKTDPDVLLMGVTSSHSTVSGLSIQEGRDF
                     TPLEDEGPRNVAILGKDVVERVFPWGSPLGRFVRIGSYRLQVVGVLEKRGAVFGESQD
                     NIVLMPLSTVLQGLDPEDNLAIAIKAKPDASLQKAMSEVRSVMRVRHKLKMTDPDTFE
                     LETKESLVAMWKNLTAAIFAAAVGIAGISLLVGGVGIMNIMLVSVKERTREIGIRKAL
                     GARPNDIARQFLIEASVLSLLGGVLGVGLSLGVLKVVSWVTDVMPLAFSVDSVVLALV
                     FSLGVGISFGYFPARKAAKLNPIEALRYE"
     gene            complement(1415..2632)
                     /locus_tag="PK710_23005"
     CDS             complement(1415..2632)
                     /locus_tag="PK710_23005"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT12669.1"
                     /translation="MIHGLMESGRLAVESLRSNTMRSLLTTLGIVIGISTVIAIISVI
                     QGLNRAFAAQISGMGSDVIYVQKWNWTSNDWTQLRKWKDIGFKELHAIQRSSHLVDKV
                     APMFVTMRKVKNGRRYIDEATVYGFGVGGPEIRGAVPMAGRALTPVDFDGSREVVLIG
                     WDIAEKLFPHESPVGKPLTVGGKRLQIVGVLEKKGNAFGESLDATVIMPIGTFMKHFG
                     RRRMMTITVVPKANADPDETIDELRGILRRVRKVPFGQPDTFGINRIDALQELYRTLT
                     GGLYAAMIGVGSISLLVGGVGIMNIMLVSVTERTKEIGIRKALGARPRAILLQFLLEA
                     IVLSCLGGVLGTWLGFGIAALVDAVSPVPASVQPWSIGIGLGFSATVGLVFGVLPARR
                     AAAKNPIDCLRYE"
     gene            complement(2629..>3197)
                     /locus_tag="PK710_23010"
     CDS             complement(2629..>3197)
                     /locus_tag="PK710_23010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT12670.1"
                     /translation="GSGKSTLMNILGCLDTPSQGRYELDGEQVAGMNENELADIRNRK
                     IGFVFQTFNLLARQSAQANVELPLVYSGVKAATRKKRAKEALEAVGLGQRADHRPNEL
                     SGGERQRVAVARALINRPSIILADEPTGNLDTRTSIEIMRLFNNLHQNGNTIVLVTHE
                     PDIAEYAHRVVRLRDGLIESDAPGVGRT"
BASE COUNT          796 a         1097 c          667 g          637 t
ORIGIN      
        1 agcgcttatc gaagtatttt caggtgggcc aaatttccgg cagcagtagt aggaagacga
       61 tagcccaaac acgatagccc cttccccccg tatcccttcc accccccaga caaccacaaa
      121 atccaagata tcgtcagcca tcatcgcctt cactcgtacc gaagcgcctc gatcggattg
      181 agtttcgccg cttttcgagc aggaaaatac ccaaacgaaa tccccacccc cagcgaaaat
      241 acgagcgcca acaccacgga atctaccgaa aaagctagcg gcatgacatc cgtaacccac
      301 gacaccacct tcaatacccc aaggctcaga ccaaccccca gcaccccgcc aagcaaggaa
      361 agaaccgaag cttcgatgag aaactgccgt gcaatatcgt taggacgcgc tcccaacgct
      421 tttcgaatcc caatctcccg ggtgcgctct ttcacactga caagcatgat attcatgatc
      481 ccaacccctc ccaccaacaa agaaatcccg gcaatcccca ccgctgccgc aaaaatcgcc
      541 gccgtcaggt tcttccacat cgccaccaac gactctttcg tctccaactc gaatgtatcg
      601 ggatccgtca tcttgagttt atgacgcaca cgcatcacgg accgcacttc actcatcgcc
      661 ttctgcaacg aggcatccgg tttggctttg atggcaatcg ccaaattgtc ttccggatcg
      721 agaccctgca acaccgtcga caacggcatc aacacgatat tgtcctggct ctccccgaat
      781 accgcccccc gtttttcaag gaccccaacg acttgcaaac gatacgagcc aatccgaacg
      841 aatcgcccga gaggagaacc ccagggaaat acacgctcca ccacgtcttt cccaagaatc
      901 gcgacattcc ttggcccctc atcctccagc ggcgtgaaat cccggccttc ttgtatggat
      961 aatccactga ccgtggaatg actgctcgtg acccccatga gcaatacgtc cggatccgtc
     1021 ttgttgttcc cactccgaac ttgctgtgac cgaaaacgga ccgaaggaga gacggattcc
     1081 accgacgggc aggcgagaag aatcgccttc gcatcatcgc tcgtcagatt cggacgcaac
     1141 cgatactctc tcatgaaacc cactcgaacg cctggatact tttgcaaaaa aaacgacgac
     1201 gcccccacac ggtcgagttc ccccgtcacc agattgttga tcccccccac gatactgagg
     1261 attccaatga ccgtagcgac gccaatgacg atcccaaggc aggtcaacaa cgaacgcaac
     1321 gcattggccc gtacggacga caacgccata cgagcatttt cccataaggt tcgcagtctc
     1381 caccaccgcg ccaaaccagc cagtagcccc ctcatcactc atacctcaaa caatcgatag
     1441 gatttttcgc cgcggcgcgc cgtgccggca aaaccccgaa cacaaggccc accgtcgccg
     1501 aaaacccaag gccaatgccg atcgaccagg gctgaaccga agcaggaacg ggacttaccg
     1561 catccaccaa ggccgcaatc ccaaacccca accacgtgcc aagaactccc cccaaacacg
     1621 acaaaacaat cgcttccaac agaaattgta acaggatggc ccgcggacgc gcccccaacg
     1681 ccttgcgaat ccctatctcc ttggtccgct ccgtcaccga taccaacatg atattcatga
     1741 tccccacccc ccccaccaac aaggaaatac tccctacccc gatcatcgcc gcatatagcc
     1801 cccccgtcaa cgtacgataa agctcctgca acgcatcaat gcgattgatt ccaaacgtat
     1861 ccggttgccc aaacgggact tttcgaacac gacgcaaaat gccccgaagc tcatcgatgg
     1921 tttcatcggg atccgcgttg gccttgggaa cgaccgtgat ggtcatcatc cgccgacgac
     1981 caaagtgttt catgaacgtg ccaatcggca tgatgaccgt ggcatcgaga ctctcgccga
     2041 aagcattgcc ctttttctcc aataccccca cgatttgcag gcgttttccc cccaccgtca
     2101 ggggttttcc aacgggcgac tcatgcggaa atagcttttc cgcgatatcc caaccaatga
     2161 gaacgacctc gcggctccca tcgaaatcca ccggtgtcaa cgctcgaccc gccatcggta
     2221 cggcaccgcg tatctccggt ccgccaaccc caaacccata gacggtggcc tcgtcgatat
     2281 accgacgccc gtttttcacc ttgcgcatcg tcacgaacat cggcgctacc ttatcgacca
     2341 gatgactgct tctctgaatc gcatgcaact ccttgaatcc aatgtctttc cacttgcgaa
     2401 gctgcgtcca atcgttgctc gtccaattcc acttttgcac atagatgacg tcactgccca
     2461 tgcccgaaat ctgcgcggca aaagcacgat tcaacccttg gatcaccgat atgatcgcaa
     2521 tcaccgtgct gatccctatc acgatcccaa gcgtggtcag aagacttcgc atcgtattcg
     2581 accgaagcga ctccaccgca agacgaccgc tctccatcaa tccgtggatc atgttctgcc
     2641 cacccccggc gcatccgact cgatcaggcc atcccgcagc ctcacgacac ggtgggcgta
     2701 ttcagcgata tccggctcat gcgtcaccag cacaatcgta ttcccattct gatgtaaatt
     2761 attgaataaa cgcatgattt ctatactcgt gcgggtatcc aggttgcccg tcggctcatc
     2821 ggccaggatg atggatggtc gattgatcaa ggcgcgagcc accgcgaccc gttgccgctc
     2881 tccaccggac aattcgttgg gacgatggtc ggctcgttgt cccagtccca ccgcttccaa
     2941 agcttctttc gcacgctttt ttcgcgtggc agctttcact ccgctataaa ccaagggcaa
     3001 ttccacgttg gcttgcgcgg attgacgagc cagcaggttg aaggtctgaa acacgaatcc
     3061 gatttttcga ttgcgaatat ccgccaattc gttttcgttc atcccggcca cttgctcccc
     3121 atccaattcg taacgcccct gcgaaggcgt atccaaacac ccgagaatat tcatcaacgt
     3181 ggattttcca gagcccg
//
LOCUS       DAOSOR010000644         3197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_30224_length_3197_cov_3.623488, whole genome shotgun sequence.
ACCESSION   DAOSOR010000644 DAOSOR010000000
VERSION     DAOSOR010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30224_length_3197_cov_3.623488"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1955
                     /locus_tag="PK710_23015"
     CDS             <1..1955
                     /locus_tag="PK710_23015"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT12671.1"
                     /translation="QPDFALPILLDPSELDQLKGPDRILVVDDNEANRDLLARRLRNK
                     GYVVETVESGKRALAMVEQEHFDLLLLDVMMPEISGFDVLKTLRESRSVSDLPIIMAT
                     ALDASEDIVKALKLGANDYVTKPLDFAVVLARVGTQLALKRTKDQVVELNRSLAEAQR
                     KISKLVDSSAEAMNDVESWSSAMALEVAQAIGAKSISVWVYEAERPSAFTQTDAPSPD
                     VQEVSVAAKNGTYLVRDSETVVPVQGLSGEVFGALVIRGLRLGELAAKVVASFAHQLG
                     GALELQKMRKDLKASTERRRASQQEMIDRGVDLLRICPTCQRCFDQRTTHCPEDNQAL
                     PEPSSLPYRVAGRHRLVRVLGEGGMGTVFAARDERLERDVAVKIIKGEHFNNDTVRLR
                     FEHEARAVAKIDHPGVVAIYDSGELEDQSLFIVMELLVGRDLSELVNACGRGTPKQVA
                     QLLRQVGSGLAAAHRVRLVHRDIKPENIFLVVESTGFRAKLLDFGVAKELAMDHGLTQ
                     TGALIGTPLFMSPEQILGKSTDTRSDIYSFAAVAYLALTGRRVTLGTTLPEILLDVVQ
                     NEPPPPSSLIIELPHSVDAAFAAALAKEPDKRPNDVESWVHGFVDDLERIESDTKGWA
                     LGNEMLHLASQKRERNTTVREKPGSS"
     gene            complement(2143..2268)
                     /locus_tag="PK710_23020"
     CDS             complement(2143..2268)
                     /locus_tag="PK710_23020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12672.1"
                     /translation="MAEKNDKGGAPETKEKYETPWAMLAIFGVIFLGIVLYGFFN"
     gene            complement(2317..2694)
                     /locus_tag="PK710_23025"
     CDS             complement(2317..2694)
                     /locus_tag="PK710_23025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12673.1"
                     /translation="MPDLANDDDPQDTHRALISRLLLRLLLMTTLAISGVVSFRVPMR
                     WAPESSRIEAPSQTECEYVSTVGDETPARGTERHELAIPVHASVGMFREEDERYLTWV
                     SEWIGYSSRRETAQRARAPPVDG"
     gene            complement(2687..>3197)
                     /locus_tag="PK710_23030"
     CDS             complement(2687..>3197)
                     /locus_tag="PK710_23030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT12674.1"
                     /translation="TILEDRNLGYALGATDYLTKPIDRKRLMTVLNRICGNTSGMPIL
                     IVEDDEFIRDTMRRMLTREGWAVVEAENGKAALRVIDTVVPALILLDLMMPEMDGFQF
                     LEELRMGDRASHVPVVVVTAKELTAQDHERLNLQAERVIRKGAYSRNDLMREIGRLVR
                     AHVRSEVNA"
BASE COUNT          721 a          891 c          850 g          735 t
ORIGIN      
        1 cgcaacctga ttttgctctt cccatcctgc tggacccttc cgaactcgac caactgaaag
       61 gaccggaccg tatccttgtc gtggatgata acgaagccaa tcgggacttg ctcgccagac
      121 gccttcgaaa caaggggtat gtcgttgaaa cggtcgaaag cggaaagcga gccctcgcga
      181 tggtcgagca ggaacacttc gacttgctgt tgctcgatgt gatgatgccg gaaatcagtg
      241 gcttcgatgt actaaaaacc ttacgagaaa gccgttccgt ttcggatctt cccatcatca
      301 tggccacggc tctggatgcg agcgaggaca ttgtcaaagc gctgaaactc ggagccaacg
      361 attacgtaac gaagcctctg gactttgcgg tcgtgcttgc tcgcgtcgga acgcaattgg
      421 ctctcaaacg taccaaggat caggtggtcg agctgaaccg aagcttggcg gaagcgcaac
      481 gcaagatttc caaactcgtg gattcctctg cggaagccat gaacgatgtg gagtcatggt
      541 cctccgccat ggcgttggag gttgcgcaag ccatcggtgc gaaaagtatc tccgtctggg
      601 tctacgaagc ggaacgacct tcggcattca cgcagaccga tgcgccatct ccggacgtcc
      661 aggaggtatc ggtcgcagcg aaaaatggta cctatctcgt tcgcgattcg gagaccgtcg
      721 tgcccgttca agggctttct ggagaagtat tcggtgctct ggtcattcga gggctgcgcc
      781 ttggagaact cgccgcgaaa gtcgtcgcta gctttgccca tcaactcggc ggggcccttg
      841 aactgcaaaa aatgcgaaag gacttgaagg cctccaccga gcgtcgaaga gcctcccaac
      901 aagaaatgat cgatcgcggc gtcgacttgt tgcgcatctg ccctacatgt cagcggtgtt
      961 tcgatcaacg aactacccat tgtcccgagg acaatcaggc cctaccggag ccgtcgtctc
     1021 ttccctatcg tgtggcgggt cgtcatcgat tagtccgggt actgggggaa ggcggcatgg
     1081 ggacggtatt tgcggcgcgc gatgagcgat tggaacgaga tgttgccgtt aaaatcatca
     1141 aaggcgaaca cttcaataac gatacggttc gcttgagatt cgaacatgaa gctcgcgcgg
     1201 tggcgaaaat cgatcacccg ggtgtcgttg cgatttatga ctccggtgaa ctcgaagacc
     1261 aatcgctttt catcgtgatg gagttgttgg tcggtcgcga cctatccgaa ttggttaacg
     1321 cttgcggaag aggtacccca aaacaagttg cacaacttct tcgtcaagta ggttccggac
     1381 tcgcggcagc ccatcgcgtt cgactcgtgc atcgggacat caaaccagaa aacattttct
     1441 tggtcgtcga gtccaccgga tttcgtgcaa aacttctcga cttcggggtc gccaaagaac
     1501 ttgcgatgga ccatggtctc acccaaaccg gcgcgctcat cggcaccccg cttttcatgt
     1561 ctcccgagca aattctcggt aaatctacgg atactcgcag cgatatttat tcctttgccg
     1621 cggtcgccta cctcgcgctt acgggtagaa gagtcaccct gggaaccaca ttgcccgaga
     1681 tccttctgga tgtcgtgcaa aatgaaccgc ctcctccttc gtctctgatc atcgagcttc
     1741 cccattcggt cgatgctgct ttcgctgccg ctctcgcgaa agaaccagac aagcgtccga
     1801 acgatgtcga atcatgggtt catggtttcg tcgacgatct cgaaaggatc gaatcggata
     1861 cgaaaggatg ggcccttggc aacgagatgc tccatctggc ttcacagaag cgagagcgaa
     1921 acacgacggt tcgggaaaaa cctggttcgt catgatgcct ccgtcgcgca caagtactgg
     1981 ttttttcctt gtccacgaag ccttcggcac gacgtagacg cgatgggtat tggttcaaac
     2041 gatagtcaga tgcgtcggcc atggcacggg aggtaacgtt acgtgcggcc ttggagaaaa
     2101 gatgggtgat ggaattgacg gtttggaatc ggaggggttc ggttagttga agaaaccgta
     2161 caagacgata cccaggaaaa tcacaccgaa tatcgcaagc attgcccagg gggtttcata
     2221 cttctctttg gtttcgggcg caccgccctt atcgtttttc tcggccatga gtcactgcct
     2281 ttctcgatcg atgaacaaca actatcgact tgagtttcat ccatcgacag gcggtgctct
     2341 tgcgcgttgt gccgtctcac gccgactcga gtatccgatc cactccgata cccatgtcag
     2401 ataacgttca tcttcctcgc gaaacatccc cacggaggca tgcacgggaa tcgcaagctc
     2461 atgcctttcc gtacctcgtg cgggagtctc atctccaacg gtcgatacat actcacattc
     2521 cgtctggctt ggcgcttcga tgcggctcga ttcgggcgcc caccgcatgg gcacccgaaa
     2581 ggaaaccacc ccagagatgg ccaacgtggt catcaacagg agacggagca acaaacgcga
     2641 aatcaaagct ctatgagtat cctgcggatc gtcgtcgttg gcaaggtcag gcattgacct
     2701 ccgaacgaac atgcgcgcgg accaatcgtc cgatttcgcg catcaggtca tttcggctgt
     2761 aggccccttt tcggatgacc cgttcggctt gtaaattgag gcgttcatgg tcttgggccg
     2821 tcaattcctt ggcggtgacc accaccaccg gcacatggct cgcccggtct cccattctca
     2881 attcttcgag gaattgaaag ccatccattt ccggcatcat caaatccagc aaaatgagtg
     2941 ccggcacgac ggtatcgatg acccgaagcg ctgctttgcc gttttccgct tccacgacag
     3001 cccagccttc ccgtgtcagc attcggcgca tcgtatcgcg tatgaattca tcatcctcga
     3061 caatgagaat cggcattccc gatgtatttc cacaaatacg attcaatacg gtcatcagcc
     3121 gttttctgtc gatcggcttg gtcaagtagt ccgtggcacc tagcgcataa ccaagattcc
     3181 gatcttcgag aatggtg
//
LOCUS       DAOSOR010000645         3194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_30261_length_3194_cov_3.437719, whole genome shotgun sequence.
ACCESSION   DAOSOR010000645 DAOSOR010000000
VERSION     DAOSOR010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30261_length_3194_cov_3.437719"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1070)
                     /locus_tag="PK710_23035"
     CDS             complement(120..1070)
                     /locus_tag="PK710_23035"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOT12675.1"
                     /translation="MNIETRWLRVERAEARDRSIEEAARLLGAGKLVAFPTETVYGLG
                     ARVGDESAVRAVFRAKGRPAAVPLIVHVRDIQQARRYVREMPEVVEELARKYWPGPLT
                     VVLDRSEQVPDAVTAGGGTVGIRVPDHPVALALIDALGEGIAAPSANPYGRLPPVRGE
                     HVIHALGGRIDAVLDGGPCRGGLESTVIDARILPAVLLRRGAMDPRQLGISLVERVVT
                     EYWAFDGWIRVGPSSEYARWAARLGALKVGRMGEPEMSRKPSDVASYARWLYEGLHEL
                     WAQGCARVWVDAPPRGPEWEPIWDRLARLSKLSPPDCPPD"
     gene            complement(1071..2549)
                     /gene="dnaB"
                     /locus_tag="PK710_23040"
     CDS             complement(1071..2549)
                     /gene="dnaB"
                     /locus_tag="PK710_23040"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOT12676.1"
                     /translation="MEIRGDGRSGTISHADDAGDSRGDRVPPHDLGAESAVLSAVLLS
                     RDVLDKVQPIVAADHFYSDANRKIFEAFVDLSANGRPIDILTVRSWLDDRGFLQRVGG
                     AKYLAEILDAVPAVSNVDDYAKRIREKWRVRELISACRRVAAEGYGTIEDVQKFIDSA
                     EQTVYDIARTPESTTVMSLRDVIQVTFTRITDSLRRGSRITGTPTGFTRLDKLTGGLN
                     EGDLTIVAARPGMGKTSLVLNMAVNVALPKEVRSEDAEGGEEYRLEQGAGVAIFSLEM
                     PREQLATRLLCSEGRVDVSKVRQGGLQRDDWSRLTKAAGELSKLPIWIDDTSDLSVLE
                     LRAKVRRIQSMQGDRGKLGLVMIDYLQLMSGNPKASSREQQISEISRNLKKLAKDLSV
                     PVIALSQLNRAVETRGTKDKRPQLSDLRESGAIEQDADTIVFIYRDDYYNPEDPSVAG
                     LAELIVAKQRNGPTGRIKVRFTKSCTRFDNLAEEEYGEEEPG"
     gene            complement(2565..2981)
                     /gene="dut"
                     /locus_tag="PK710_23045"
     CDS             complement(2565..2981)
                     /gene="dut"
                     /locus_tag="PK710_23045"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOT12677.1"
                     /translation="MAGDEAHLPECKTEGAAGADLSASIPEPWTIEPGQRVLVPTGIA
                     LAIPQGFEGQIRPRSGLALRHGVTVLNAPGTIDSDYRGELKVLLINLGERPFVIEPGD
                     RIAQLVIAACACPRFEVVWELDETQRGAGGYGSTGL"
     gene            complement(3014..>3194)
                     /locus_tag="PK710_23050"
     CDS             complement(3014..>3194)
                     /locus_tag="PK710_23050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOT12678.1"
                     /translation="QVLTGGGALSDIQLFSQLKALGEVKTALSSMTDPRARELEKRVA
                     QLLEASLRTGRFAQL"
BASE COUNT          702 a         1150 c          693 g          649 t
ORIGIN      
        1 cccgccctat aaaaatcatg gggtgcttgt tttcaatcgt ttcccggaac agaactcggc
       61 ttataggctt ctgagaccac gccttcacgt cgcttgccga cgatggtagg cgtgtatttt
      121 cagtctggcg gacagtccgg cggagacaac ttcgacaggc gcgccaaccg atcccaaatc
      181 ggttcccact ccggcccccg cggcggtgcg tccacccaaa ctcgcgcaca cccctgtgcc
      241 cataattcgt gcaatccttc gtacaaccat cgagcgtagg aagccacatc cgaaggcttg
      301 cgcgacattt caggctcgcc catgcgccca accttcaggg cacccaacct cgctgcccat
      361 cgtgcatact cggaagacgg ccctacacga atccatccat caaaggccca atattccgta
      421 accactcgtt ctaccagcga aattccaagc tgccgcggat ccatcgcccc tcgacgcaac
      481 agcaccgcag gcagtatccg cgcatcgatg accgtcgact ccagcccacc acgacatggg
      541 ccaccatcaa gcaccgcatc gatccgtcct cccaacgcat ggatcacatg ctccccccga
      601 acaggcggaa gcctgccata cggattggcg ctcggcgccg caattccctc ccccaatgca
      661 tcaatcagcg ccaaagccac cggatgatcc ggtacccgaa tccccaccgt acccccccca
      721 gccgtcaccg catccggcac ctgctccgaa cggtccaaga caaccgtcaa cggccccggc
      781 caatacttcc gcgcaagctc ctccaccacc tcaggcatct ccctcacata acgtcgcgcc
      841 tgctgtatgt cccgaacatg cacgatcaaa ggcacggccg ccggacgtcc tttggctcga
      901 aaaaccgccc ggactgcgga ctcgtcgccc acccttgccc ccaacccata caccgtctcc
      961 gtgggaaatg ccacgagctt acccgcgcca agcaaacgcg cggcctcttc aatactccta
     1021 tcccgagctt ccgcccgttc gaccctcaac catcgcgtct cgatattcaa tcagcccggc
     1081 tcctcttcgc catactcttc ttccgccaag ttatcgaatc gcgtacagga cttggtaaag
     1141 cgtaccttga tccgccccgt gggaccgttg cgctgcttgg caacaatgag ttccgctagc
     1201 cccgccaccg atggatcctc cgggttatag taatcatctc gatagatgaa aacgatcgta
     1261 tcggcgtctt gctctatcgc accagactca cgcaaatccg aaagctgcgg gcgcttgtct
     1321 ttcgtcccac gcgtttctac cgcacgattc aattgcgaaa gggcaatcac cggcaccgaa
     1381 agatccttcg ccagtttctt caaattcctt gagatttcgc ttatttgctg ctcgcggctc
     1441 gaggctttcg gattgccgct catgagctgc aaatagtcga tcatcaccaa tcccaacttg
     1501 ccccgatcgc cctgcatcga ttgaatgcga cgtactttgg cgcgcagctc gagaaccgaa
     1561 aggtccgacg tatcatcgat ccaaatcggc agcttggaaa gctctcccgc ggctttcgtc
     1621 aaccgactcc aatcgtcacg ctgaagcccc ccctgccgca ctttgctcac atccactcgg
     1681 ccctcagaac acaataggcg ggtagcaagt tgctctcgcg gcatctccaa cgaaaatatg
     1741 gccacccccg ctccctgctc caaccgatac tcctcccccc cttccgcatc ctcgctcctt
     1801 acctctttcg gcaacgccac gttgaccgcc atgttgagta ccagcgacgt ctttcccatt
     1861 cccggtcgcg ccgccacaat cgtcaaatcc ccttcgttca acccacccgt caacttatcc
     1921 aagcgcgtaa aacccgtcgg cgtccccgtg atcctactgc cccgacgcaa cgaatccgtt
     1981 atccttgtga acgtcacttg aatgacatcc cgcaaagaca tgaccgtcgt cgattcaggc
     2041 gttctcgcga tgtcataaac cgtttgctcg gcactatcga tgaacttttg aacgtcttcg
     2101 atcgtcccat atccctccgc cgccacccga cgacacgcgg aaatcaattc tctaacacgc
     2161 cacttctccc gaattcgttt tgcataatcg tcgacgttcg aaaccgcagg aaccgcatca
     2221 agaatttccg caagatactt cgcacctccc acacgctgca aaaacccacg atcatcgagc
     2281 cagcttcgaa ccgtcaaaat atcgatcggc cgaccgttcg cggataagtc aacgaaagcc
     2341 tcgaagattt ttcgattggc atcactgtaa aaatggtccg cggcaacaat gggctgcacc
     2401 ttatccaata cgtcccgcga caataagacg gcggatagca ccgccgactc cgcccccaga
     2461 tcatgcggcg gtacacgatc gccgcgactg tctcccgcat cgtcagcatg cgatatcgtg
     2521 ccagatctcc catcacccct tatttccacg gcctttcctt tctttcacaa acctgtcgac
     2581 ccgtaaccac cagccccccg ctgggtctcg tccaattccc ataccacctc aaaccgcgga
     2641 cacgcgcagg ctgcaatcac gagttgagcg atcctgtcgc ccggctcgat gacgaaagga
     2701 cgctcgccca agttgatgag cagcactttc agctcgcctc gataatcact gtcgatagtc
     2761 cctggcgcat tgagcacggt caccccatgg cgcaacgcaa ggcctgatcg tggacgtatt
     2821 tgcccttcga acccttgtgg aatcgccaac gcaattcccg tgggaaccaa aacccgctgc
     2881 cccggttcga ttgtccaagg ttcgggaatc gacgcagaca aatcagctcc cgcggctcct
     2941 tcggttttgc attccggaag atgagcctcg tcgccagcca cgtttctcac tcgaatgaca
     3001 agatcacgag acgtcacaat tgcgcgaatc gccccgtccg aagagacgcc tccaagagct
     3061 gagccacgcg tttttcgagt tccctcgcgc gcggatccgt catcgaggaa agagccgttt
     3121 tcacctcacc gagggctttg agctgactga aaagctggat gtcgctcaat gccccccctc
     3181 ccgtcaaaac ctgt
//
LOCUS       DAOSOR010000646         3186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_30348_length_3186_cov_4.281060, whole genome shotgun sequence.
ACCESSION   DAOSOR010000646 DAOSOR010000000
VERSION     DAOSOR010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30348_length_3186_cov_4.281060"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PK710_23055"
     CDS             <1..495
                     /locus_tag="PK710_23055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12679.1"
                     /translation="SGGSGGSGGSGGSGGSGGSSTAVDCSFTNSCQSARDIGTVRGDK
                     GSDSLQVTGATSEWLRVRVLEANESVVGQKLKVTITLQPANQTNFDLFVFVNKGSDVI
                     ECSDLAGSSMETLSKPDTVSVSWGEGSVANGDDDHRWVSIEVRHKTGECDTTKKWTLT
                     VKGN"
     gene            complement(502..2010)
                     /locus_tag="PK710_23060"
     CDS             complement(502..2010)
                     /locus_tag="PK710_23060"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HOT12680.1"
                     /translation="MTKRTVTSWALVGLFFAGSMLGCGSDEATTDGNVCVPTAEICDG
                     KDNNCNGQIDENLTQECTEGGLKGEQTCTNGQWGPCVTQQPECVPSPEVCDGKDNDCD
                     DAIDEDDDGNALQRECKDDQGLAGKQICQKGAWGPCQASCVPSAEVCDGKDNDCDGLV
                     DEDDSGQPLKQDCSNQCGLGTEICVAGTWQQCTAPQPSTEVCDGVDNDCDGEVDEDFE
                     CAKGTQANCGTDVGVCEFGTKVCGATCQWGNCIGGVNPGTEICEGKNDEDCDGTVDNG
                     CGCTNGEKKSCCGGTEITCTGGTWPSCPPAPVEVCNGIDDNCNDKIDEDLPVNPYQLD
                     EDISGIDDCAHAKALTAIMEGEPAQSFTFHLYKPDGSPDRDFFLFRADENSGVDDTFG
                     CIADGPNECYTLVIKMTSVPAGVTYDYCVYDVGFEGDTATCAKPIKKTCASSSTPNNQ
                     VTMNWAGLCSLNDNRYFYLEVFPKSGSALSCQPYKFTIEHLDNSPQEAECSF"
     gene            2164..>3186
                     /gene="nagZ"
                     /locus_tag="PK710_23065"
     CDS             2164..>3186
                     /gene="nagZ"
                     /locus_tag="PK710_23065"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOT12681.1"
                     /translation="MQSLSRLCGQLIVGGYRGEEPSATFLASLRRGERAGAILFKRNL
                     PTWQAGYVASAMIADASSDEVGPVVAVDQEGGRVQRFGSPVLRLPPMRQLAQRADETL
                     VAEVAETLGLQLRQLGFNVNFAPVLDVDTNPANPVIGDRSFSSDANVVARFGVAFALG
                     LQAAGVAACGKHFPGHGDTTIDSHLDLPTVDTSPQRMQQVEFIPFYAAIEAGIDALMT
                     AHIVVRQVDSERPATMSPTMVRGWLREQMGFRGVVFSDDLEMRAIAGRMEIGQAAVQA
                     VRAGCDVLLICKREDWQDEAHASLVREAESSQEFRGYCEQAVARCRALRARRPRKAAP
                     HWRQIDF"
BASE COUNT          654 a          909 c          882 g          741 t
ORIGIN      
        1 agcggcggct cgggcggtag tggagggagc ggcggctcgg gtggcagcgg cggttcgagc
       61 acggctgtcg attgctcttt cacgaattca tgccaatccg ctcgagatat cggcaccgta
      121 cgcggtgaca aaggaagcga ctccctgcaa gtgaccggag caacttccga atggcttcgc
      181 gtgcgcgttc tcgaagccaa cgaaagcgtt gtcggtcaaa agctcaaggt cacgattacc
      241 ctacagccag caaaccaaac caacttcgat ctatttgttt tcgtcaacaa aggaagcgat
      301 gtcatcgagt gctccgatct cgccgggtca tcgatggaga ccttgagcaa acccgatacg
      361 gtcagcgtct cttggggaga aggatccgtg gccaacggtg acgacgatca ccgatgggta
      421 tccatcgaag tccgtcacaa gacgggggaa tgcgatacca caaaaaagtg gacgcttacg
      481 gtgaagggga attgatggcg gttagaacga gcattccgct tcttgcgggg aattgtcgag
      541 gtgttcgatc gtgaacttat aaggttggca actcaacgcg gagcccgact tggggaagac
      601 ttcgagatag aagtacctgt tgtcattcag cgagcaaagc cccgcccaat tcatggtcac
      661 ctggttgttc ggcgttgacg aggaagcgca ggtcttcttg atcggcttgg cacacgtggc
      721 cgtatcgcct tcaaaaccaa cgtcatacac gcagtagtcg taggtgactc ccgccggaac
      781 gctcgtcatt ttgatgacca acgtataaca ctcattggga ccgtcggcga tgcagccaaa
      841 ggtgtcatcc accccgctgt tttcgtcggc gcggaacagg aaaaagtccc gatcgggtga
      901 accgtccggc ttgtatagat ggaaggtaaa gctctgagcc ggttcccctt ccatgatggc
      961 cgtcagggcc ttggcatggg cgcaatcgtc aatgcctgaa atatcctcgt cgagttggta
     1021 cggattgacc ggcaagtcct catcgatctt gtcgttacag ttatcatcga taccattgca
     1081 gacttcgaca ggggccggtg ggcaggacgg ccacgttccc cccgtgcagg tgatttccgt
     1141 tccgccacaa cagctctttt tctctccgtt cgtgcagcca cagccgttgt ccacggtgcc
     1201 gtcacagtct tcgtcgtttt tgccttcaca tatctccgtg cccggattga ctccaccgat
     1261 gcagtttccc cattggcagg tggcaccaca aaccttggtg ccaaattcgc atacgcctac
     1321 gtcggttcca caattggctt gcgtgccctt ggcacattcg aagtcctcat cgacttctcc
     1381 gtcacaatcg ttatcgacac catcgcaaac ctcagtggaa ggttgtggtg ccgtgcattg
     1441 ctgccaggtg cctgccacgc aaatctccgt tcccaagccg cattggttcg aacaatcctg
     1501 ttttagcggt tgcccagaat catcttcgtc caccaggccg tcgcaatcgt tgtctttgcc
     1561 atcgcaaacc tcagcgctcg gcacgcagga agcttgacac ggtccccagg ctcccttctg
     1621 acagatttgc tttcccgcaa ggccttggtc gtccttgcat tctcgctgaa gcgcattccc
     1681 atcgtcatct tcatcgatgg catcgtcgca atcgttgtct ttgccatcac acacttccgg
     1741 tgagggtacg cactcgggct gttgcgtcac gcacggtccc cattgaccgt tcgtacacgt
     1801 ctgctctccc ttgagccccc cttccgtgca ttcctgcgtc aagttctcgt caatctgacc
     1861 attgcagttg ttgtccttcc catcgcaaat ctcagccgtc ggaacacata cgttaccgtc
     1921 cgtcgttgcc tcatcacttc cacatcccag catggaaccg gcaaagaaca acccaaccaa
     1981 cgcccaagaa gtaacggttc gcttcgtcat gattcagtcc tcaattcccc cgaaaaaaca
     2041 caattgtatt gaagtcccgc atccggcctt cgtcaaggaa ggaatcgatt gattgggaag
     2101 cgcaaagatg gaattgccgt agtgttgaat ttctcagccg ctcgaccaca ctcttgcacc
     2161 ataatgcagt cgctttcgag attgtgtggc caattgatcg ttggcggtta ccgcggtgaa
     2221 gagccttccg ctacctttct tgcctctctt cgacgagggg agcgggccgg ggctatcttg
     2281 ttcaagcgaa acctgccgac ctggcaagcg ggctacgtcg ccagcgccat gatcgccgac
     2341 gcctcatccg atgaggttgg cccagtcgtt gccgtcgacc aggaaggagg acgtgtacaa
     2401 cggtttggtt cacctgtatt gcgtcttcca ccgatgcgtc agttggcgca acgggcagat
     2461 gaaacccttg tggcagaggt tgccgaaaca ctgggacttc aacttcgaca actcgggttc
     2521 aacgtcaact tcgcaccggt gttggacgtg gacacgaacc ccgccaatcc tgtcattggc
     2581 gaccggtcgt tttctagcga tgcgaacgtg gtggctcgtt tcggggtggc gtttgccctc
     2641 gggctgcaag ccgcgggagt ggctgcctgt ggcaaacact ttccgggaca tggggatacc
     2701 accatcgatt cgcatctcga tctgcccacg gtggatacct cgccccaaag aatgcaacag
     2761 gttgaattta ttccatttta tgctgctatc gaggctggaa tcgatgcgct gatgaccgcg
     2821 catatcgtgg ttcgccaggt cgattcggag cgaccggcga cgatgagtcc caccatggtt
     2881 cgcggatggt tacgcgaaca gatgggcttt cgaggggttg tgttttccga tgatttggag
     2941 atgcgcgcga tagcggggcg catggagatc ggacaagcgg cggtacaggc ggttcgagcc
     3001 ggttgtgacg ttctgctcat ttgtaaacgt gaggattggc aagacgaagc ccatgcatcg
     3061 ctggttcgtg aggcggagtc tagtcaggag tttcggggct attgcgagca agccgtggca
     3121 cgttgtcgcg cgttgcgggc tcgtcgccca cgaaaggccg ccccgcattg gaggcaaatc
     3181 gacttt
//
LOCUS       DAOSOR010000647         3175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_30480_length_3175_cov_4.682692, whole genome shotgun sequence.
ACCESSION   DAOSOR010000647 DAOSOR010000000
VERSION     DAOSOR010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30480_length_3175_cov_4.682692"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PK710_23070"
     CDS             <1..317
                     /locus_tag="PK710_23070"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOT12682.1"
                     /translation="TAHAEIIALRLAAAATGHWRLSDCTLVVTLEPCPMCAGAIVNAR
                     IPRLVFGCLDPKSGGVRSLMRIADDERLNHRAHIVEGILAEECAQRLKDFFGRLRRDG
                     KK"
     gene            complement(406..2670)
                     /locus_tag="PK710_23075"
     CDS             complement(406..2670)
                     /locus_tag="PK710_23075"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HOT12683.1"
                     /translation="MSLRRRLLLLSFLLAVAATFAAALLLPPFIRHQAQRRAQQYHLT
                     LQIQSVRPAWLGLRLGQVDIACPDIPSLTLHAEAIEVRRSSIAVINASATVQTDLRSF
                     AEQVHTWRSRLPSSSESNPAKKSSALVAIDNLALSWQGFDGEGSNCSLTGLNIRDLFG
                     GQSVDVGLVQIEHPQGSLKAEAVALAWIREPSAAIRSLGIGRAYADVSVDHELQGASS
                     KSAAVSASDFRDRLARWASTVWAPMAVGATVRVEALEVLVRHGGQQLHVGPGRLSVQQ
                     DKTALLLDLTPRSEGAAGITFRGSLPLSVGPVVLDVVGGPIPLGALGVKEGNLGLVDV
                     ASTTLSSNAHLKLHEDGKTLSFDGKVVAKNVGLEHARLAPAPVRGLELAAKLRGVASL
                     DGSMLRFDEGQIDIGAVQFSLRGSLDRGETSAVDIHFGIPLVACQSVLEALPPSLAPV
                     VHGMIAAGTLSLSGYLRWDETDPKKYRFEYKADHDCRFTSVPEQVDVRRFRSVFKRKA
                     YDLQGKPIEVETGPGTAGWVSREGFNHFIEAAVMTCEDGRFRRHRGFDHEAIENSVRE
                     NLRAKKMLRGASTISMQLAKNLYLGREKTVSRKLQELILTMYLEQTLTKDQIMELYLN
                     VIEFGPMTYGIGNAASKYFHKHAASLTLGQSMYLASVLPSPLRQHFAKDGKVTDGWMR
                     YLYKLMRIAAKMRWITELELEDGLGEWVVYGTPDPIRMTPMHEDEGEPLDDPSLNPPK
                     DDPFGWQPDGSLVY"
     gene            complement(2764..2880)
                     /gene="rrf"
                     /locus_tag="PK710_23080"
     rRNA            complement(2764..2880)
                     /gene="rrf"
                     /locus_tag="PK710_23080"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(2961..>3175)
                     /locus_tag="PK710_23085"
     rRNA            complement(2961..>3175)
                     /locus_tag="PK710_23085"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          702 a          961 c          869 g          643 t
ORIGIN      
        1 ctaccgccca cgcggaaatc attgctctac gcctcgcggc cgccgccacc ggacactggc
       61 gattgtccga ctgcacgctc gtggtcaccc tcgaaccctg cccgatgtgc gcaggagcca
      121 tcgtcaacgc ccggatccca cggctcgtgt tcggatgcct cgaccccaaa tccggcggcg
      181 tacgctcgct gatgcgcatc gccgacgacg agcggctcaa ccatcgcgcc catatcgtgg
      241 aaggtattct agcggaagag tgcgcccaac gattgaaaga cttctttggc cgtctgcgac
      301 gcgacggcaa aaagtgaatc cggtggcgat agagaccaca gtatcgcacc tgttgtccat
      361 tcccccagcg cgatggactc gccccccttg gacaaattat ccaaatcagt ataccaacga
      421 cccatcgggc tgccatccga acggatcgtc tttcggtgga tttagggatg gatcgtcgag
      481 gggctcgccc tcatcctcgt gcatcggcgt catccggatc ggatcaggcg tgccatagac
      541 cacccactca cccaaaccat cctccaattc caactcggta atccaacgca ttttcgcggc
      601 aatacgcatt agcttgtata aatatcgcat ccaaccgtcg gtcactttgc catccttggc
      661 gaagtgttgg cgcagcggtg atggcaaaac ggacgccaag tacatcgatt gtccaagggt
      721 gagagaagcg gcatgtttgt ggaagtactt ggatgccgca tttccgattc cgtaggtcat
      781 tggtccgaat tcgatcacat tgagataaag ctccatgatt tggtctttgg tgagcgtttg
      841 ctcgagatac atggtgagga tgagttcttg caacttgcgc gaaacggttt tttcccttcc
      901 caggtagaga tttttcgcga gttgcatcga aatcgtactg gcgcctcgca acatcttttt
      961 ggctcgtaaa ttctcccgaa ccgagttttc gatggcctcg tgatcgaacc cacgatgtcg
     1021 tcgaaatcgc ccatcctcac aggtcatcac cgccgcttcg ataaaatgat tgaacccttc
     1081 tctcgagacc catcccgcgg tacccggccc ggtttccacc tcgatcggct tgccctgaag
     1141 atcgtaggcc tttcgcttga agacactgcg aaaccggcgc acatccactt gctcgggtac
     1201 cgaagtgaat cggcagtcgt gatcggcctt gtattcgaat cggtattttt ttgggtccgt
     1261 ctcgtcccat cgaagatatc cagataagga cagagtgccc gctgcgatca tcccgtgtac
     1321 taccggtgcc agtgaaggcg gtaacgcttc gagcaccgat tggcaggcca ccagtggtat
     1381 accgaaatgg atatctaccg cagaggtttc ccccctgtcg agactgcccc gtagagaaaa
     1441 ctgaacagcc ccaatgtcga tctgcccctc gtcgaaccgc agcatcgaac catccaacga
     1501 ggccacccca cgaagcttgg cagccaactc caatcctcgc accggtgccg gagccagacg
     1561 ggcatgctcg agccccacgt ttttcgctac caccttccca tcgaaggaaa gggttttgcc
     1621 atcttcatgc agtttcagat gggcattcga agacagcgtg gtcgaggcca catccaccaa
     1681 cccgagattt ccttctttga ccccgagcgc ccccaaagga atgggccccc ccaccacgtc
     1741 gagcaccacc ggcccgacag aaagcggcaa agatccacga aaggtaatgc ccgccgctcc
     1801 ttcggagcga ggagtcagat ccaaaagaag cgccgttttg tcctgctgaa ccgatagacg
     1861 cccaggccca acgtgaagct gctgcccccc atgacgcacc aacacctcca acgcttcgac
     1921 ccgcaccgtc gcccccacgg ccatcggtgc ccataccgtc gacgcccacc gcgcgagacg
     1981 atcccgaaag tcacttgccg acacggcagc cgatttcgac gacgccccct gcagctcgtg
     2041 atccacgctc acgtccgcat acgcgcggcc gatccccaaa gagcggatgg ccgccgacgg
     2101 ctcgcgtatc cacgccaacg ctacggcttc ggctttcaag gagccttggg ggtgctcgat
     2161 ttgcaccagt cccacgtcta cagattggcc accgaaaaga tcgcggatat tcagcccggt
     2221 cagcgaacaa ttcgagcctt cgccatcgaa gccctgccac gatagggcaa gattatcgat
     2281 ggcgacgagg gcggaacttt tttttgccgg gttcgattcg gacgacgagg ggagtcggct
     2341 tcgccaggta tgaacctgct cggcaaagga gcgaagatcg gtttgtacgg tggcagaagc
     2401 gttgatgacg gcgatggagg agcgacggac ttcgatggcc tcggcgtgaa gagtgagcga
     2461 agggatatcc ggacaagcga tgtcgacctg tccgaggcgc aggccgagcc aggcaggtcg
     2521 aacggattgg atttgaaggg tgaggtggta ttgctgggcg cgtcgttggg cctggtgccg
     2581 aatgaagggg ggtagcagca acgcggcggc gaaagtcgcg gcaacagcga gcagaaagga
     2641 caagagcaac agccgtcgac gaagggacat cgtgggggta ataccgcgtt gcgagacgag
     2701 gtgaaaggcg gtggacaaaa agaaaaagct ccgacttctt tcgaaggcgg agcttctata
     2761 taagcccggc agcgtcctac tctcccacac agtcgcccat gcagtaccat cggctccgaa
     2821 gagcttgact tccgagttcg ggatgggatc gggtatggcc tcttcgatat cgccaccgga
     2881 aaattttggg atccaatgac cggagttggg tcataacccc aacttcgcgt acgaatgaga
     2941 ttcgatgagt gtattagggg acggtcaagc ctcacggctt attagtactg gttagctccg
     3001 cgcattgcta cgctttcaca cccagcctat taccacgtgg tctacatgga gcctttaggt
     3061 ctttcgacgg gacacctagt cttgaggccg gcttcccgct tagatgcttt cagcggttat
     3121 ccgttccgaa cgtagctacc cggcaatgct cttggcagaa caaccggatc accag
//
LOCUS       DAOSOR010000648         3167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_30587_length_3167_cov_4.832905, whole genome shotgun sequence.
ACCESSION   DAOSOR010000648 DAOSOR010000000
VERSION     DAOSOR010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30587_length_3167_cov_4.832905"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PK710_23090"
     CDS             <1..528
                     /locus_tag="PK710_23090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12684.1"
                     /translation="LVTLPTGTGAGNDPNAENASAVASASPARYAWDGAIFANNDSVL
                     IGGADLAYVAGGLTVQAEATYLQFIRIRGDNVQKDELRSLLTTGLFAGYFLIPELSVG
                     AELRYGHWLSTPAAVEKNSVARNNLSAGGGVRGYFKLGDSISLRPGLSYHQALDEPLS
                     DAEYKYVQLDLPFVF"
     gene            836..1714
                     /locus_tag="PK710_23095"
     CDS             836..1714
                     /locus_tag="PK710_23095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HOT12685.1"
                     /translation="MRSCVQLAEILESIVATCDVPARLALDPVGVVRTYRHPAQQELA
                     GLLSASLAFGQVKALRRAIEQVLSRMGSDWMGVLEDRHETLCRLEGFQYRMVRGRDIG
                     RLLVGARFVQRRQGSLGEAFCKEFHLAGALRPALVAWTRQLRDAAGAFDHEDQGRRGY
                     GHVLPDPAGASPNKRLLLFLRWMVRPNDGVDLGLWTTVPPSALLIPVDTHIHRLGKLL
                     GMTQRSNPSWLAAEDITAVLRQIDPDDPVRFDFALCHLGMTLGCSTRPDPTRCESCPV
                     RADCQQRAQPRMAESL"
     gene            complement(1734..>3167)
                     /locus_tag="PK710_23100"
     CDS             complement(1734..>3167)
                     /locus_tag="PK710_23100"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOT12686.1"
                     /translation="LVRLGVSSVDVRHVDDGVLITLATSQRHNPALLASIDEMMLSIP
                     HLVCVAVSERDRSDPQVLGSQPTVVRGPDAARYTPDRHAPYHYAGPGAFTQAHPESMT
                     QLHRDLEEQIIRSFGRIEGCRVLELYAGTGAFSLRLAHAGAHVTLVESFAPSVQLAAR
                     AATEQNLALETRIGDAAVVTQQLLRNHAFFDLIIVNPPRRGLAPAVRTAVISLQPRMA
                     LYVSCNPITLARDVAVWTYHGLRPRMIRPYDMIPLSEVVETMAVLTPAVPTQPTLLYS
                     DPQLLVVDKPAHEKLLASPGAPGSLLERVRQTLGAPDAQPLHPMDVGQSGVCLLARTA
                     AAKNALLQDVLPRQSSWTVLAKGIPHAKGSLGKTKAAHPGPPMRYVRQQVIAGHGWLQ
                     VFTQGDDAQGMARGLSRIGHPIVGDEKFGDRRTNQYFIHRHGLDRPFMHLDTVTVFHQ
                     GRTLQFRSELAADLQAVADDLRASAEE"
BASE COUNT          607 a          884 c          986 g          690 t
ORIGIN      
        1 ctggtcacgc tgccgacggg tactggggcg ggcaacgatc ccaatgcgga gaatgccagc
       61 gccgtagcat cggcgagtcc tgcccgatac gcctgggatg gtgccatttt cgccaacaat
      121 gattcggtcc tcatcggtgg tgcggatctc gcgtatgtgg ctggcgggct gaccgtgcaa
      181 gcggaagcta cgtatctgca attcattcga attcgcggcg acaacgttca gaaggacgaa
      241 ctccgcagtt tgctgacaac cgggttgttc gccggttatt ttctcatccc cgaactatcc
      301 gtgggtgccg aacttcgcta cggccattgg ttgagcacgc cagccgccgt cgaaaaaaac
      361 agtgtcgcgc gcaataacct ttcggcggga gggggtgttc gcggttactt caaactcggc
      421 gactccatat cgctgcgacc agggttgagc tatcatcaag ctctcgacga gcctctcagc
      481 gacgccgagt acaagtatgt ccagcttgat ttgccgttcg ttttctgatc ggcgcgggta
      541 gccatccaat agccgcacgc ggagcacggc cccgcatagc cataacgata aaatatcctt
      601 atcatttcta tggttatgct tgatttgccg ccccggagcc aacccgtccg acgaacgctt
      661 ggagatttgg cgcgggtaag cctttcggcc attttccgat ggacgagggg ccccttccag
      721 atcagtcgca acatattgat ttaacaagat ttaatttgaa ttcgccagcg ttggtattct
      781 ccctatcgct ccttccgctt gccaatcacc tccccccgcc cgatgaatca ttcccatgcg
      841 ctcttgcgtg caactggctg aaatactcga atcgatcgtc gccacctgtg acgtgccggc
      901 ccgtctggct ctcgaccccg taggcgtcgt gcgaacgtat cgtcatcccg cccagcagga
      961 attggcggga ttgctttccg catctctcgc cttcggtcag gtgaaagccc tgcgacgcgc
     1021 gatcgaacag gtgctttcgc gaatgggcag cgattggatg ggtgtgctcg aggatcggca
     1081 cgaaacgttg tgtcgtttgg aggggttcca gtaccggatg gttcgagggc gggacatcgg
     1141 ccgtttgcta gtgggcgcca ggttcgtaca acgtcggcag ggctcgttgg gcgaagcatt
     1201 ttgcaaagaa ttccacctgg cgggagcgtt acgacccgcc ctcgtagcgt ggacgcgaca
     1261 gttgcgcgat gccgccggtg ctttcgacca tgaggatcag gggcgacgcg gttatggtca
     1321 tgtgctgccc gacccggccg gggcaagccc aaataagcga ctcctgcttt ttctacgctg
     1381 gatggtgcgt ccgaacgatg gtgtcgactt gggattgtgg accacggtcc cgcccagtgc
     1441 gctgttgatc cccgtcgata cgcatattca tcgcctggga aaactccttg gcatgaccca
     1501 acggtccaac ccaagctggt tggcagcgga ggatatcacc gcggtattgc gtcaaattga
     1561 ccccgacgat cccgtgcgtt tcgactttgc tttatgccat ctcgggatga cgcttggctg
     1621 ttcgacccgc cccgatccca cacggtgtga aagctgtccg gttcgagccg attgccaaca
     1681 acgagcgcaa cctaggatgg cggaatcgtt ataactattt gaaataacaa tatttattct
     1741 tctgccgacg cacgcaggtc gtcggcaacg gcctgcagat ccgctgcaag ttcgcttcga
     1801 aactgaagcg tcctgccctg gtgaaatacc gtcaccgtat ccagatgcat gaacggccga
     1861 tcgaggccgt gacggtggat gaagtattgg ttggtgcgcc ggtcgccgaa tttttcgtca
     1921 cccacgatgg gatgaccgat tcgtgacagg ccgcgagcca tcccctgagc atcgtctcct
     1981 tgcgtaaaaa cctggagcca tccgtggccc gcgatgactt gttgtcgaac gtagcgcatg
     2041 ggcggaccgg ggtgtgctgc tttggttttt cccagggagc ctttggcgtg cgggatgcct
     2101 ttggcgagga cggtccaact gctttggcgt gggagaacgt cttgaagtag cgcatttttc
     2161 gctgcagccg tacgggccag caaacaaacg ccgctctgtc ccacgtccat cggatgaaga
     2221 ggttgggcgt cgggtgcgcc taacgtttgc cggacccgtt cgagcaagga gcctggagcg
     2281 cctggggaag cgaggagttt ttcgtgggct ggtttatcga ccacgagcag ttgggggtcg
     2341 gaatacagca gggttggctg ggttggtaca gccggagtca gcacggccat tgtttccacc
     2401 acttcggaca gggggatcat gtcgtagggg cgaatcatcc gtgggcgtag gccatggtat
     2461 gtccacacgg ctacgtcgcg cgccaatgtg attgggttgc aagacacgta aagcgccatg
     2521 cgcggttgaa gagaaatcac ggccgttcgt acggcgggag cgagtcctcg tcgcggcgga
     2581 ttgacgatga tgaggtcgaa aaaagcatgg tttcgaagga gttgctgggt cacgaccgcg
     2641 gcgtcgccga tgcgggtctc gagggcgaga ttttgttccg tggcagctcg agccgccagt
     2701 tgaacggaag gcgcaaagct ttcgacgagt gtgacgtggg cgccggcgtg cgccagtcga
     2761 aggctaaacg ctcctgttcc cgcgtaaagt tcgagcaccc gacatccttc gatacgaccg
     2821 aaagaacgaa tgatttgttc ttccaggtct cggtgcaatt gcgtcatgga ttccgggtgg
     2881 gcctgggtga aagctcctgg tccagcatag tgataaggcg catgccgatc gggggtgtag
     2941 cgcgcggcgt cggggccccg aacgacggtg ggttggctgc cgagcacttg cggatcggaa
     3001 cggtcgcgct cgctgaccgc cacacagacg aggtggggaa tcgacagcat catctcatcg
     3061 atcgacgcga gcagcgcggg attgtggcgc tggcttgtgg caagcgtgat cagcacgccg
     3121 tcgtcgacgt gccgcacatc cacgctgctc acaccgagtc ggacaag
//
LOCUS       DAOSOR010000649         3162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_30673_length_3162_cov_3.908915, whole genome shotgun sequence.
ACCESSION   DAOSOR010000649 DAOSOR010000000
VERSION     DAOSOR010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30673_length_3162_cov_3.908915"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..576
                     /locus_tag="PK710_23105"
     CDS             85..576
                     /locus_tag="PK710_23105"
                     /inference="COORDINATES: protein motif:HMM:NF002449.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HOT12687.1"
                     /translation="MSCPCGRGPSLSECCGPYLKGIKKPITAEDLMRSRYAAYVVQDI
                     DYIIKTHAPETVHTVDREDATAWSQNADWLGLDVLRTERGEKDDEEGIVEFVARYELD
                     GDLHTHHERSRFRRIDGTWFYVEGEMVKPEPMVRTGPKVGRNDPCPCGSGKKYKKCCG
                     KVA"
     gene            655..2301
                     /gene="pgm"
                     /locus_tag="PK710_23110"
     CDS             655..2301
                     /gene="pgm"
                     /locus_tag="PK710_23110"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HOT12688.1"
                     /translation="MALHELAGKPAPRELLVHVPRLVTAFYTHRPDPNEPSHRVSFGT
                     SGHRGSSLDHSFNEHHILAIVQAICEYRASEGITGPLYLGMDTHALSEPAQAVAIEVL
                     AANGVDTRITKEFTPTPVISHAILTYNAAKTQGLADGIVITPSHNPPADGGIKYNPPE
                     GGPADTKTTSLIQQRANQILEQGLSAVQRIPYERALRCACIREHDYIAGYVGDLDRVI
                     DMQAIAQAGLNIGVDPLGGSGIHYWDPIADRYGLKLEVVNRVVDPTFSFMTVDKDGKI
                     RMDCSSPYAMAGLIEHKDRFDIAFGNDPDFDRHGIVTRTSGLLNPNHYLAVAIHYLFR
                     NRPSWKPEAAIGKTLVSSSMIDRVAVSLGRTLSEVPVGFKWFVQGLITGQYGFGGEES
                     AGASFLRKDGTVWTTDKDGILLDLLAAEITAREGKDPGALYRELEAKHGSPVYERMDA
                     PATPSQKQALAKLSPEQVRAETLAGEAIIARLTKAPGNDASIGGLKVVTANGWFAARP
                     SGTEDIYKIYAESFLGTGHLHEIQQQAQQIVHDAFQVAGV"
     gene            2862..>3162
                     /locus_tag="PK710_23115"
     CDS             2862..>3162
                     /locus_tag="PK710_23115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12689.1"
                     /translation="MKKMIVIGLMGMLASMAWACSDDKDDDNNNGGGGGGTNTGGGSN
                     VGGGSNVGGGTAEGGSGGGSNVGGGTAEGGSGGGSNVGGGTAEGGSGGGSNVGGGT"
BASE COUNT          674 a          853 c          957 g          678 t
ORIGIN      
        1 tcgagcgatc ggcgcgcgcg ggtcttgcaa accttgcttg ctcatgcttg gtttgggttc
       61 tctttttcct cgaggagatg aaacttgagc tgtccctgcg ggcgaggccc ctccctttcg
      121 gaatgctgcg gcccttatct gaagggtatc aaaaaaccga tcacggccga ggatctcatg
      181 cgttcgcgat atgcggcgta tgtggtgcag gacatcgatt acatcatcaa aacgcacgca
      241 ccggaaaccg tacatacggt cgaccgggag gatgctaccg cgtggtcgca gaatgccgat
      301 tggcttgggc tcgatgttct ccgcaccgaa cgaggggaaa aagacgatga agaggggatc
      361 gtcgagtttg tggcgcggta cgaactcgat ggagatttgc atacgcacca cgagcggtcc
      421 cgattccgac gaatcgatgg gacttggttt tatgtcgagg gggaaatggt caagccggaa
      481 ccgatggttc gaacgggtcc gaaagtaggc agaaacgatc cttgtccctg cggcagtggc
      541 aagaagtaca aaaaatgctg tggaaaggtg gcgtgagatt tccgcctctg cttgcggctg
      601 cctttatcaa tctcgtttgg taaataagat ccttctgacc acggaggtcg atttatggcc
      661 cttcatgagc ttgccggcaa accggctcct cgcgaattgc tcgtccacgt tcctcgcctc
      721 gtcacggcgt tttataccca tcgtcccgat cccaacgagc cctctcatcg ggtcagtttc
      781 ggtacttccg gccatcgcgg ctcgtcgctc gaccacagtt tcaacgagca ccatatcctc
      841 gccatcgtgc aagccatctg cgaataccgg gcgtcggaag gcatcaccgg tccgctgtac
      901 ctgggcatgg atacgcacgc gttgtcggag cccgcacaag cggtggcgat cgaggtgcta
      961 gcggccaacg gggtcgacac gcgcatcacg aaggaattca ccccaactcc cgtgatttcc
     1021 catgccatcc tcacctacaa cgcggcaaaa acgcaggggc ttgcggatgg catcgtgatc
     1081 acgccttcgc acaatccgcc tgccgatggt ggtatcaagt acaatccgcc ggaaggcgga
     1141 cccgcggata cgaaaaccac cagcctcatc caacagcgtg ccaatcagat cctcgagcaa
     1201 ggattgtccg ctgtccaacg aatcccttat gaacgcgcgt tgcgctgcgc gtgcattcga
     1261 gagcatgatt atatcgccgg gtatgtcggc gacctggatc gggtcatcga catgcaggcc
     1321 atcgcgcaag cgggcttgaa catcggggtc gatccgctgg gtggctcagg catccattat
     1381 tgggatccca ttgcagaccg ctacgggttg aaactcgaag tggtcaatcg cgtggtcgat
     1441 ccgacctttt ctttcatgac cgtcgacaaa gacggcaaaa tccggatgga ttgttcttcc
     1501 ccctacgcga tggcggggct catcgaacac aaggatcgat tcgacatcgc ctttggcaat
     1561 gacccggact tcgaccggca cggcattgtc acccgcacga gcggactgtt gaaccccaac
     1621 cattatcttg cggtcgcgat tcattatcta tttcggaatc gtccgtcgtg gaagccggag
     1681 gcggcgattg gcaaaacgct cgtttccagt tcgatgatcg accgggtggc cgtgagtttg
     1741 ggcagaacgc tgtcggaggt tcccgtgggc ttcaagtggt tcgtgcaagg gctcatcacg
     1801 gggcaatacg gttttggggg tgaggagagc gcgggggcgt catttttgcg caaagacggg
     1861 accgtgtgga cgaccgataa ggatgggatt ttattggatt tattggcggc cgagatcacg
     1921 gcgcgggagg gcaaggatcc cggtgcgttg tatcgggagt tggaagcgaa gcatggcagt
     1981 ccggtgtatg agcgcatgga cgcgccagcg acgccatcgc agaaacaggc tcttgccaag
     2041 ctttcccctg aacaagtgcg ggccgagacg ttggcgggcg aggccatcat cgccaggctc
     2101 acgaaagctc cgggcaacga tgcttcgatt ggcggattga aggtggtgac ggccaatggt
     2161 tggtttgcgg cccggccctc gggcaccgag gatatttaca aaatatatgc ggaaagcttc
     2221 ctcggcacgg gccatttgca cgagattcaa cagcaggcgc aacagatcgt tcacgacgct
     2281 ttccaagtgg caggggtgtg ataacgctac aaacccctcg cgttcccacg tcacactatc
     2341 cgtttccgga tactcgtcct tgtcgatggc caaacgcgcg gcgcaggcga cagggggcga
     2401 gactcgataa ccccgcgagg ccctcgcggg actgcaagac agctcatgca tccatcatcg
     2461 ctatcgaatc attacccggg taaccttcat gattctgccg ttcggccttc gcatccgggg
     2521 gatagcaacg gccacatcga cattaaatcc aataaattca aatcgttaac ttgatttccc
     2581 tgggttgacg attccttgga agggtcctgt tgccgtgctt gaaggattcg tggtcgtcat
     2641 tttgtcccca tcctgaccca atgttttgtc gtcataaatt aagcagacca cctttgcagc
     2701 gccttgcggt tgtcttatga ccgccctagt ttcactcgct gtcgggaatt tgcccctccc
     2761 ttgtcggtat tgcaattcgg aggaaaacgc ttttggggga ggaagggata ttcgtgacgg
     2821 tgcgtatcag gaaatacctt aacaacgaag ggaatatttc aatgaaaaag atgatcgtga
     2881 tcggtttgat ggggatgctg gcttccatgg cctgggcttg ttccgacgat aaggacgatg
     2941 ataataataa cggtggcggc ggcggaggca ccaacaccgg aggcggcagc aacgtgggtg
     3001 gcggcagcaa cgtaggtggt ggtactgccg aaggcggctc cggaggcggc agcaacgtag
     3061 gcggtggtac tgccgaaggc ggctccggag gcggcagcaa cgtaggcggt ggtactgccg
     3121 aaggcggctc cggaggcggc agcaacgtag gcggtggtac tg
//
LOCUS       DAOSOR010000650         3146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_30867_length_3146_cov_4.603688, whole genome shotgun sequence.
ACCESSION   DAOSOR010000650 DAOSOR010000000
VERSION     DAOSOR010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30867_length_3146_cov_4.603688"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3146)
                     /locus_tag="PK710_23120"
     CDS             complement(<1..>3146)
                     /locus_tag="PK710_23120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12690.1"
                     /translation="EYCLHAGTHLSVLFANKVVLIGGQILKGSRSAYEAALDLGEILS
                     WCGGSELAIARDITMKELKAFADALSEAQRGKRTDSFESPTRKVRLRSVSASARLRGV
                     DVEPMTVEQKIVRTYASAVVVMRRLFDDLRRGQYVLPPKIKRIAQSLVDLSEGKTPAF
                     LGVTEVRNQNHDDAGRAVNTAILSVATARQVTANRVLLCQIAMAALMHDIARPRALAQ
                     LTGGLPGVVPRLSEDAEDRQAAGAAAVLTALGRVNEPTVARTVVTYESLWIRRGQMLG
                     PLYGGLREPTVHARIIAIARKYNDFVTPEPGLPPPAPDVAIARIFSQMEHPTDRLILR
                     MLVAALGLMPVGTLVKLSSGETAEVVVGPDLGAPTDRPRIKIIMDGRGGMVSPPRVVD
                     LSNPPADLKGAHIQQVLSTEGWGRGIDLESAVGEQASEIPAPPSSAPAHAAASFSDSA
                     YEPGDYADEVGATDSVIPDSMVDDSGQSALSNSANRSLGWHDAPPPAARSAEQALLRE
                     TVRLPTARQMMPTARPPKVYTLPSTAETLDPDADENTVFEASPFGEQPDGAPYDDDDE
                     AGTIFFQRASSPPVVVGAKLREQAGIPPTAQGELAKTPLVHVLVYVLDRELSGSLVVM
                     DPEGREHCVFFDHGAPSKVRTEHPVAMLGEELIASRALPPAVIDTMVQQAQAHRRLLG
                     EFLIHSGLVTRQAVTDALRAQVTHRLDWLAKLPGEARYEFYRDQNLLASWGGQITPIE
                     PLSAILSAMRSWNDHERIISNLMRLKDHPIRLHPEACVQGLMLSQEEQQVLSVIRSRE
                     VSLLELYGDSLSTDHGISAFLYMLFATRQLQIPGQDRAPMRCSQHTAFLPNEAISSRR
                     PEAIHPAPSVTTVAGQEDDELSYESYPSLATSPSAVAQAVVSDMKATITYRSDVGSAQ
                     EPVLGDDLVPQGLEPTARGKLASTPLIHVLTYMLDHLATGTVVLREPDGVFHAVYFEN
                     GAVAKVRSGRPMARLGELLVAAGQLDAGQVDAAVQTAKEIGTLLGEYLIVDNLSSRGA
                     VIHALENQIFEKIICLANL"
BASE COUNT          718 a          969 c          857 g          602 t
ORIGIN      
        1 agattcgcca agcagatgat cttttcgaag atttggtttt cgagagcatg gatgacagcg
       61 ccacggctcg atagattgtc gacgatgaga tactcgccta gcagagtgcc aatctctttc
      121 gcggtttgca cggccgcatc cacctggccg gcatccaatt gtccagccgc aaccaatagc
      181 tctccgagcc gagccatcgg tcggcccgac cgaaccttgg ctaccgcccc attctcgaaa
      241 taaaccgcgt gaaaaacacc atcgggctcg cgaagcacaa ctgtccccgt ggccagatga
      301 tccaacatat acgtaagcac gtggatgaga ggggtgctcg ccaacttgcc acgggctgtc
      361 ggctctaatc cctgcggcac caagtcatcg cccaacacag gctcttgagc agaacccaca
      421 tcggatcgat acgtgatcgt cgctttcatg tcggaaacga ctgcttgtgc caccgccgat
      481 ggactcgtcg ccagcgacgg atacgattca tacgacaact cgtcatcttc ttgtcctgcc
      541 acggtcgtga ccgaaggcgc cgggtggatg gcttctggtc gtcgcgaaga aatggcttcg
      601 ttgggtaaga atgcggtgtg ctgactgcaa cgcatcggcg ctcgatcctg cccggggatc
      661 tgaagctgtc gcgtagcaaa tagcatgtac aaaaaagcgc taatgccgtg atccgtggac
      721 aagctatcgc catacagttc gagcaacgac acctcacggg agcggatgac cgaaaggact
      781 tgctgctcct cctgggacaa catcaacccc tgcacgcagg cctcgggatg cagacgaatc
      841 ggatgatcct tcaatcgcat caagttggaa atgatgcgtt cgtggtcgtt ccaagagcgc
      901 atggcgctca aaatggcgct cagcggctca atcggcgtga tctggcctcc ccaagaagcc
      961 agcaagttct gatcacgata aaattcgtac cgcgcctcgc ccggaagctt cgcaagccaa
     1021 tcgagccgat gcgtaacctg tgctcgcaga gcatctgtca ccgcctgccg cgtgaccagc
     1081 ccactatgaa tgagaaactc accaagcaaa cgccgatggg cttgggcttg ttgcaccatg
     1141 gtgtcgatga cagcaggagg taacgcgcgt gaagcaatga gttcctcccc gagcatcgcc
     1201 accggatgct ccgttctcac cttcgacggc gcaccatggt caaaaaacac acaatgctcc
     1261 cgtccctccg ggtccatcac gaccagagag ccggataatt ccctatccaa tacgtagacc
     1321 aacacgtgaa cgagaggagt ttttgccagc tccccttgcg cggtaggcgg tatgcccgct
     1381 tgctcacgga gcttcgcccc aacaacgaca ggtggactcg acgctcgttg gaaaaaaatc
     1441 gttcccgctt cgtcgtcatc gtcatacggc gcgccatccg gctgctctcc gaacggcgaa
     1501 gcctcaaata ccgtattctc atccgcatcg ggatccagcg tttcagcagt cgagggcagc
     1561 gtatagactt tgggcgggcg cgcggtcggc atcatctgcc gggccgtcgg caaacgcacc
     1621 gtttcgcgaa gcaacgcttg ctctgcagaa cgcgcggctg gtgggggggc atcatgccat
     1681 cccaaagacc ggtttgcgct attcgacagc gcactttgtc ctgaatcatc gaccatggaa
     1741 tcaggaatga ccgaatcggt ggctcccacc tcatcggcat aatccccagg ttcataagcg
     1801 gaatcagaaa acgatgcggc agcgtgcgca ggcgcggaag aaggaggtgc cggaatttcg
     1861 gacgcctgtt cgccgacagc cgactcgagg tcgatgccgc gcccccatcc ttccgtactc
     1921 aaaacctgct gaatatgtgc gcctttcaag tccgcagggg gattggataa atcgacgaca
     1981 cgcggcggcg aaaccattcc gccgcggcca tccatgatga tcttgattcg tggccggtcc
     2041 gtcggcgccc ccagatcggg ccccacgaca acctccgccg tctctccaga cgacaacttt
     2101 acgagcgttc caacaggcat gagcccaagc gccgccacga gcatgcgcaa gatgagccgg
     2161 tcggtaggat gctccatctg ggaaaaaatc cgtgcgatgg ccacatcggg cgcgggagga
     2221 ggcaaccccg gctcgggtgt cacaaaatca ttgtatttcc tggcaatggc aatgatacgc
     2281 gcgtgaaccg tgggttcacg taacccgcca tagagcggtc ccaacatctg accgcgacga
     2341 atccacaacg actcatacgt caccaccgtg cgcgccaccg taggctcgtt gacccgacca
     2401 agggccgtaa gcacagccgc ggctcccgcg gcttggcggt cctccgcatc ttcgctcaat
     2461 cgaggcacca caccgggcag cccccccgta agctgcgcga gggcgcgcgg acgagcgata
     2521 tcgtgcatca acgctgccat ggcaatctgg cagagcaaca cgcggttggc agtgacttga
     2581 cgcgcggtag ccacggataa aatggccgtg ttgaccgcgc gacccgcatc atcgtgattc
     2641 tgattgcgaa cctcggttac cccaagaaat gctggtgttt tcccttccga tagatccacc
     2701 agactctggg ctatgcgttt gattttcggc ggaagaacgt attggccgcg tcgaagatca
     2761 tcaaacaatc gcctcatcac cacgaccgcc gaggcatacg ttcgaacgat tttttgttcg
     2821 acggtcatgg gttcaacatc gacacctcgc aaacgtgcgg aagcagaaac agaacgaaga
     2881 cgcacctttc gggtcggaga ttcgaaagaa tccgtgcgtt ttcctcgctg tgcttccgaa
     2941 agcgcatcgg cgaaggcctt gagttccttc atcgtgatgt cccgagcgat ggccaattca
     3001 gaaccaccac accacgacag gatctcccca agatcgaggg cagcctcata agcgctgcgg
     3061 gaacctttga ggatttgacc gccgatgagc accaccttat tggcaaaaag cacggataga
     3121 tgtgtgcccg catgcagaca atactc
//
LOCUS       DAOSOR010000651         3140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_30965_length_3140_cov_3.546191, whole genome shotgun sequence.
ACCESSION   DAOSOR010000651 DAOSOR010000000
VERSION     DAOSOR010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30965_length_3140_cov_3.546191"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..632
                     /locus_tag="PK710_23125"
     CDS             108..632
                     /locus_tag="PK710_23125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12691.1"
                     /translation="MRNTILLLMGLSLLFIQSSMFWLLGWVPVSGLTPSLILPLIVFM
                     GVHEYSLARGAALACVMGYGLDLLAAAPVGLFTFVSVATFVLARAAGVRLAAQHIITQ
                     VPLAFVFSLVQSVMVLVLLAIFGANPHGAREMLSLVLPHAIGTALFSPIVFRIASKLH
                     QTVGVPPRAERTPS"
     gene            629..2542
                     /gene="mrdA"
                     /locus_tag="PK710_23130"
     CDS             629..2542
                     /gene="mrdA"
                     /locus_tag="PK710_23130"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOT12692.1"
                     /translation="MSMLLSRTDFAEFRRRPRWLALGVVLGLLIMTVRLFFLQIVDGD
                     KYKALAKESVIRKTTLATTRGVIRDRFGRVLAASRPSYNVYCVPSRLDLVETWPKLVE
                     YMQLTSGEQEQLAKRIETLRHGPRKDWQILLKEDVSRDVVAVLDTHSGELRGVEVVPT
                     PVRYYPFKEMGAQVLGYMSEVDSDLLLRLRNIGYMEGDRRGAAGVERGWESYLRGRRG
                     WEKVVVDARGRRHNTPEASQLIDDPKRQDPVPGRDIRLTIDLDIQKAIHAAMRGHLAG
                     AAVVVEVRTGRILGMVSKPSYDPNDLSGGNGKTALRDAYKRAYNDPLRPIVDKTMSGA
                     YPPGSTFKPFTALAALNERLVDARQSTRCRGFHQFGNRVFKCTHVHGNVDLHKSIVES
                     CNVYYYQLGERIGMDRIAKIGLEFGFGQKTGLGTNPEAAGRMPTRAWYAQHYRQFRQG
                     FTLNAAIGQGATTVSVLQLALAYAALANGGTLYEPQIVRAVETADGTIVQDFSPRVRR
                     RVAARSEDLTLIKRALWEVVNSDRGTAYRERLPGIDVAGKTGTAQVSHRTVRAGEDTK
                     IWYFNRDHAWFTGYAPAAAPEISLAVLIEHGGAGGKYAAPVVFQVARAFEQQKADRAA
                     KSSTDTRSGAGAP"
     gene            2539..>3140
                     /locus_tag="PK710_23135"
     CDS             2539..>3140
                     /locus_tag="PK710_23135"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOT12693.1"
                     /translation="MNLHLGNQGLRTKDQFDWPLFICAALIAVVGIVNLYSATSVYGA
                     SRSELYLNQVYWLVAGGILAVIVASLDYRFLEQAGYVIYAVGVLLLIIVLILGKDIRG
                     SARWIPIGSFSFQPSEFMKLALTIALAKYLHTDPKSEARTLRDLIVPTLLTLLPVVLV
                     QQQPDLGTSIILALIFGSILVLTRIHWKTSLFSIVSVSSVA"
BASE COUNT          609 a          872 c          916 g          743 t
ORIGIN      
        1 atttatcaga cggtcgaggc ggttcctgcc gtggacttat cgcgtctcga ctatgtattg
       61 atcgtgactt ccatggagtc cgacaagggc acggacgccc cctagccatg cgcaacacga
      121 ttttattgct catgggcttg agcctgctgt tcatccaatc gtcgatgttt tggctgttgg
      181 gatgggtgcc cgtatccgga ctcaccccct cgctcatctt gccgctcatc gtctttatgg
      241 gcgtgcatga gtattctctg gcacggggcg cagcgcttgc ttgtgtcatg ggctacggtc
      301 tggatttgct cgcggcggct cccgtggggt tgttcacttt cgtgtcggtg gcgacgttcg
      361 tgctggctcg cgcggccggc gtacgactcg cggcgcagca catcatcacg caagttccgc
      421 tggcgttcgt tttttccctg gtacaaagcg tgatggtgct ggttttgctc gccatcttcg
      481 gggccaatcc gcacggagcg cgcgaaatgc tcagtctggt gcttccccac gccattggca
      541 cggcgctgtt ctcacccatc gtctttcgaa tcgctagtaa gctgcatcaa accgtcggtg
      601 ttccccctcg cgcggagagg actccgtcat gagtatgctg ctgtctcgca cggacttcgc
      661 tgaatttcga cgccgtccgc gttggctcgc tctcggtgtc gtccttggct tactcatcat
      721 gaccgtgcga ctgttttttc ttcaaatcgt cgatggtgac aaatacaagg cgttggccaa
      781 agagagcgtc attcgaaaaa cgactctggc aacgacgcgt ggtgtgattc gagatcgttt
      841 tggacgggtg ctcgcggcca gccgtccttc ttataatgtc tactgcgtgc cttcgcggtt
      901 ggatctcgtg gagacgtggc cgaagctggt ggagtacatg cagttgacca gtggggagca
      961 ggagcaactc gcaaagcgca tcgaaacgct tcgtcatggt ccgcgaaaag actggcaaat
     1021 cctgctcaaa gaagacgttt ctcgcgatgt ggtggcggtt ctcgatacgc atagtggtga
     1081 actgcgcggt gtcgaagtcg ttcccactcc cgtccgctac taccctttca aggagatggg
     1141 agcgcaagta cttggatata tgtcggaagt cgatagcgac ctgcttttgc gtctgcgaaa
     1201 catcggatac atggaagggg atcgtcgggg ggccgcagga gtggaacgcg gatgggaaag
     1261 ctatcttcgt gggcgtcgag gatgggaaaa agtggtggtc gacgcaaggg gacgtcggca
     1321 caacacgccg gaagcttctc aactcatcga tgatccgaag cgccaagacc ccgttcccgg
     1381 gcgtgacatt cgtctgacga tagacctgga catccagaag gcgatccacg cagcgatgcg
     1441 aggacacttg gcgggtgcgg cggtggtcgt ggaggtgcgc acgggacgaa tcctcggcat
     1501 ggtatccaaa ccgtcttatg atccgaacga tctttcggga ggaaacggca aaaccgcgtt
     1561 acgcgatgcc tacaagcgag cgtataacga tcctcttcgt cccatagtgg acaagacgat
     1621 gagtggggct tacccaccgg gctccacgtt caaaccgttc acggctctgg ctgcgctcaa
     1681 cgaacgtttg gtggatgcac gtcaatccac gcgttgccga ggattccatc aatttggcaa
     1741 ccgtgttttc aagtgtaccc atgttcacgg caacgtggac cttcacaagt ccatcgtgga
     1801 atcgtgcaac gtttattatt atcaactcgg tgagcgaatt ggcatggatc gcattgccaa
     1861 aatcgggctc gaattcggat ttggtcaaaa aacaggtcta ggaacgaatc cggaagctgc
     1921 ggggcgaatg cccacgcgcg cttggtatgc gcagcattac cgtcagttcc ggcaaggatt
     1981 cacgctcaac gccgccatcg gtcaaggagc cactaccgta tccgttctgc aactcgctct
     2041 cgcgtatgcg gctctcgcca acggtggcac gctgtatgag cctcagatcg ttcgagccgt
     2101 ggaaaccgcc gacggaacga tcgttcagga tttttcgcct agggtacgtc gtcgtgtcgc
     2161 cgcgcgttcg gaagatctga ctctgatcaa acgagcgctt tgggaggtcg tcaattccga
     2221 tcggggaacg gcctatcgcg agcgattgcc tggcattgat gtagccggga aaacgggtac
     2281 ggcacaggtt tctcatcgaa ccgtacgagc tggtgaggac accaagatat ggtatttcaa
     2341 tcgagatcac gcatggttca cgggctatgc tccggccgcg gcgcccgaga tatctcttgc
     2401 ggtgctcatc gagcacggcg gagcgggagg taagtacgcc gcccctgtcg tgtttcaggt
     2461 ggcgcgtgct ttcgagcaac agaaggctga tcgtgcggct aaatccagca cggataccag
     2521 gtctggtgcg ggagccccat gaaccttcac cttggcaatc agggactgcg cacgaaagac
     2581 caattcgatt ggcccctgtt catctgtgcg gcgctcatcg ccgtcgtggg aatcgtcaac
     2641 ctgtacagcg ccacgagcgt ctatggtgcc tcgagaagcg aactatatct caaccaggtg
     2701 tattggctcg tggctggggg cattctcgcg gtcatcgtgg catccctcga ttaccgtttt
     2761 ctcgagcaag ccggttacgt catctatgcg gttggtgtgt tgctgctcat catcgtgctc
     2821 attttgggca aagacatccg aggctcggcc cgctggatac cgataggttc cttcagcttt
     2881 cagccaagcg aattcatgaa gctcgcgctc acgatcgcct tggccaagta ccttcacacc
     2941 gatccgaagt ccgaagcgcg aacgttgcgc gatctcatcg tccccacgtt gctgaccttg
     3001 ttgcctgtgg ttctcgtgca acagcaaccc gatcttggaa cctccatcat tcttgccctc
     3061 atcttcggtt ccatcctcgt gttgactcgc atccattgga aaacgtcgtt gttcagcatc
     3121 gtctcggtgt cttcggtggc
//
LOCUS       DAOSOR010000652         3137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_31010_length_3137_cov_4.325762, whole genome shotgun sequence.
ACCESSION   DAOSOR010000652 DAOSOR010000000
VERSION     DAOSOR010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31010_length_3137_cov_4.325762"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="PK710_23140"
     CDS             complement(<1..822)
                     /locus_tag="PK710_23140"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HOT12694.1"
                     /translation="MTRNKPNGLRVGQEPSHNGTHPARDHDVDRGAHASGKAKFDLGV
                     GNGWFPSEDIPWGYGENRITAMARDPDWLYVYWEVTDDAIDQARQRLGRGAHDTWCCL
                     RVYDTSGRDFDGCNALSYFDIAIERTTRDWFVHVGKPRSSCHVEVGIKSRLGDFQPMA
                     RSGRTDFPRKRPVEEVSVEWLTVEDEPSKQASLPASAAYVSRYSGPAPDIPVPLQPPY
                     SDAYPRSSGATRVIMERHVERQEIQSRTWSERKVFRWFSRASRRFEITPPRSSIPW"
     gene            complement(1041..>3137)
                     /locus_tag="PK710_23145"
     CDS             complement(1041..>3137)
                     /locus_tag="PK710_23145"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOT12695.1"
                     /translation="GGVGGAGGTGGFLEEECPSNVLCGTNGVCCEVGQECVDGACMPA
                     CDSGIRCGVDATTCCTAGDVCLSDECVTPGVSCKDSYDCDEGEFCEPTLGRCLPQPSG
                     EDLCEYRPPTLPFNPVVKWSWTGSTIQPSANQVINMPVVVDLDQDNVPDVVIVTSETY
                     ESNNPGYLRALDGKTGVEKWDASADVYAAGNEVNPRGTPAAADLDGDGVVEIVAPKMG
                     GGAIAFRADGSLYWRSTLADGQTPYTEAMNSVTTAIAHMDGDGSPEIVMGGVVLDAQG
                     RVRSGQGRGRAGANDANYGAVSIIADVDGDGNQDVVTGAAAWSVTGDVIWENGMSDGY
                     PAISDLDEDGKPELIVISQGQVRVHDAATGKMLATVKMPGNGRGGPPTVADFDGDGRM
                     EIASANGTAYAVFEYESQPSPSLSVKWSKETQDLSSNVTGSTVFDFEGDGAAEVVYGD
                     ECYMRVYSGKNGDVLFETASSSATIHEYPVIVDVDGDNRTEIVVVSNDRNHGPGTCPG
                     YGMSEKARRGVFVYGDANDKWVRTRRIWNQHAYHITNILADGVLPKPEPASWKPPVGF
                     NNYRVSTQGAGVFNAPDLRVDLEVSSASCPLGLELRARVKNAGSLGVPAGVQAKFFLG
                     ADASGVFVGEGYTTKSLLPGQSEVVSVLFEAANHVPPFQFYVVVDGSTAAAGMMECVE
                     DNNDAHAGGVACPSVK"
BASE COUNT          700 a         1091 c          731 g          615 t
ORIGIN      
        1 ccagggaatc gatgaacgcg gcggcgttat ctcgaatcgt cgagacgcgc gcgaaaacca
       61 acgaaatact ttcctctccg accaggttct cgactgtatt tcttggcgct ccacatgccg
      121 ctccatgatc acacgggtgg cgccggaaga ccgcggatat gcgtcggaat acggaggctg
      181 aagcgggacg ggaatgtccg gggccgggcc cgaatagcgc gatacatagg cagcggaggc
      241 cggcaacgac gcctgtttcg atggctcgtc ttcgaccgta agccactcca cggacacttc
      301 ttcgacagga cgcttacgcg ggaaatccgt acgacccgag cgagccatgg gctgaaaatc
      361 acccagccgc gacttgatgc ccacctcgac atgacaactc gagcgaggct tacccacatg
      421 cacgaaccaa tcgcgagtcg tacgctcgat cgcaatatcg aaataactca acgcattgca
      481 gccatcgaaa tcccgccccg atgtgtcgta cacccgcaaa caacaccacg tatcgtgggc
      541 cccacgacca agacgctgcc gcgcctggtc gatcgcatca tcggtgactt cccagtagac
      601 gtagagccag tccggatcgc gcgccatcgc ggtgatgcgg ttttcgccat atccccaggg
      661 aatgtcctcg gatggaaacc aaccattgcc cacacccaga tcgaacttgg ccttgcccga
      721 cgcgtgcgca ccacgatcca catcatgatc ccgagccgga tgcgtaccgt tgtgcgaggg
      781 ctcttgaccc acacgtaaac cgttaggctt gttcctggtc atggtcactg cctgccgaaa
      841 ggatgccgtc gcttgggttt gcgctcagtg taactctttt cacccacgac aagcgcggta
      901 gaagagaaaa ccgcgtttgc tggactcctc gagtcgaaaa acgtgaaagg atacctcggc
      961 acaccctttc cgcagcatcc ccgcaaaccg tggggggcta gcatgataac caattgtttt
     1021 tattagataa atttatatat tcacttgaca ctcgggcacg ccactccccc cgcatgggcg
     1081 tcgttgttat cctccacgca ttccatcatg cccgcggccg ccgtgctgcc atccaccacc
     1141 acataaaact ggaagggagg cacatgattc gcagcttcga aaagcacgct gaccacctcg
     1201 gattgtccag ggagcaaact cttggtcgtg tatccctccc ccacaaacac gcctgacgca
     1261 tccgcgccca agaaaaactt cgcctgcacg ccagccggca cacccaagga acccgcgttt
     1321 tttacccgcg cccgcaactc cagccccagg gggcacgagg ccgacgacac ctccaaatcc
     1381 acccgcagat ccggagcatt gaatacacca gccccctgcg tggacacacg atagttgttg
     1441 aaccctaccg gcggtttcca acttgccggc tcgggcttcg gaagcacgcc atcggcaaga
     1501 atattcgtaa tatgataggc atgctgattc cagattcgcc gcgttcgcac ccatttgtcg
     1561 ttcgcatccc catagacaaa caccccgcga cgagcttttt cgctcatccc ataccctggg
     1621 catgttcccg gaccatgatt ccgatcattg gaaaccacca cgatctccgt ccgattatcc
     1681 ccatccacat ccacgatcac cggatactcg tggatggtcg ctgaactcga tgcggtttca
     1741 aacagcacgt ctccgttttt cccgctgtaa acccgcatat agcactcatc cccatacacc
     1801 acttccgccg ccccatcccc ctcgaagtca aacaccgtgg aacccgtcac attcgacgac
     1861 aagtcttggg tctctttcga ccatttcacc gatagcgaag gggaaggctg ggattcatac
     1921 tcgaaaacgg cgtaagcagt gccgttggcg gaggcgattt ccattctccc gtccccatcg
     1981 aagtcagcca ccgtgggcgg accaccccgc ccgttgccag gcatttttac cgtcgccagc
     2041 atctttcccg tggccgcatc gtgaacgcgt acctgccctt gagaaatcac gatgagttcc
     2101 ggcttaccat cctcatcgag atccgaaatc gcaggatacc catcggacat cccgttttcc
     2161 cagatcacgt cgcccgtcac cgaccacgcc gccgcacccg tcaccacgtc ctggtttcca
     2221 tccccatcca catcggcaat gatgctcacc gccccataat tcgcatcatt cgcccccgct
     2281 cgaccacgcc cttgccccga tcgaactcgc ccctgcgcat ccaacacaac cccacccatc
     2341 acgatctccg gcgaaccatc cccatccata tgagcaatcg ccgtcgttac gctgttcatc
     2401 gcctcggtat acggggtctg accatccgcc aatgtcgatc gccaatacaa cgatccatcg
     2461 gctcgaaacg caatggctcc gcctcccatt ttcggtgcca cgatctccac caccccatcg
     2521 ccatccaaat ccgccgccgc tggcgttccc cgaggattga cttcattgcc cgcagcatac
     2581 acgtccgccg acgcatccca tttttcgacc cctgtcttgc catcgagcgc tcgtaagtac
     2641 ccagggttgt tggattcata cgtctcgctc gtgacgatga ccacatccgg aacgttgtct
     2701 tgatccagat ccacaaccac aggcatgttg atgacttggt tggccgaagg ttggatcgtc
     2761 gacccggtcc acgaccactt cacgaccgga ttgaacggca aggtaggggg acggtattca
     2821 cataaatcct cgcctgaagg ctgagggaga caccgaccca gggtaggctc acaaaactcc
     2881 ccctcgtcac aatcgtatga gtccttgcac gacacaccgg gcgtcacaca ttcatccgaa
     2941 agacagacgt cgcctgcagt acagcaagtc gttgcgtcga caccgcatcg aatccccgaa
     3001 tcacaggcag gcatgcacgc gccgtccaca cactcttgcc ccacctcgca gcaaacaccg
     3061 ttcgtgccgc aaagaacgtt ggaaggacat tcttcttcga ggaatccccc ggtaccgccc
     3121 gctcctccca ctcctcc
//
LOCUS       DAOSOR010000653         3133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_31069_length_3133_cov_3.684860, whole genome shotgun sequence.
ACCESSION   DAOSOR010000653 DAOSOR010000000
VERSION     DAOSOR010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31069_length_3133_cov_3.684860"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2180
                     /locus_tag="PK710_23150"
     CDS             <1..2180
                     /locus_tag="PK710_23150"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT12696.1"
                     /translation="DDALESRLLRRTVFRSFLAADAQRGPLCLVFEDLHEAHDESLAL
                     LQFLLEHLDGPVLALCIARNELETRRDGWRSIRKERHRVIDLGPLGETDAASMMQALL
                     APCGEAPQPLVDAACQLAGGNPLLLEQMVRIFHDTGVLEEEDALSETPVWLVHLGKLE
                     TVRLPLTVEDAVQARVAALAPALRLLLEQAAAVGSVFWLGALTVLARLDSTTPELWSL
                     QDDADIRKIRELLQELMDRDFVLRLPDSTFPGDEEYVFKHNLERERVAKLTSPSVSRR
                     YHRLIADWLEHQSNTRSDEEYVAMLASHREAAGSMREAAMAYLDAGDVARSRYSPAKA
                     IEHYARGLELLGDDEPRRRLEVYHHQGDVCQQLGKMEEALVSFRSMLSLAYRLNLFGK
                     GGAAHNRIGRLHRETGVLDQAARHLGTGLALFEACNDERGIASSLDDIGKLHWLRGDY
                     PLALEQMRRALTMRRQIGDRRSIALSLNNVGLVLQDSGEFKEALESFSQALKIRREIG
                     DLLGVIITLNNLGTVAQDQGNHARALEVFEEALAVAREVGDRNKIALVLTNLGEAQRS
                     MGQASEAVLSLTEAVTTCEDLNDRLGVAEALRALGMAYLEQNELVQARESISRAVDIF
                     AAARSKVHLGSALRTLGEITAGGGWGREHASKARDYFQRSIAIFEEIGNELELARSLT
                     SYADFLDRGELVDHIVEDSRVLRHRAEGIFLKLRNSSPTNHAS"
     gene            2170..>3133
                     /locus_tag="PK710_23155"
     CDS             2170..>3133
                     /locus_tag="PK710_23155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT12697.1"
                     /translation="MQANSAQPNTLHQRLQQVASVISGQFVGKDEIIRLLLVAVIAGE
                     HAVLLGPPGTAKSALIRSLSQLLQARYFEYLLTRFTEPNEIFGPVDIAAFREGSYRRR
                     TEGMLPEAEIVFLDEVFKSNSAILNALLTLLNERRVVVGGQAIPCPLISVFGASNEVP
                     GDETLNAIFDRFLIRVHSDNLDAYHFSELLSRGVQHELRAMKQQALHPLVSSTELMSL
                     STMVGQRMQFNESFLSSYKGLVFQIRAEGISLSDRRVVKLLKLFAANAYLDGRQAADA
                     SDFFVLKHIWNNEDQVPVLAGIVQPVLEAFYREYPDRKRVGAAGV"
BASE COUNT          615 a          738 c         1006 g          774 t
ORIGIN      
        1 cggacgacgc tctcgagtct cggttgctcc gtcgcacggt gtttcggagt tttcttgcgg
       61 ccgatgccca gcgaggcccc ctttgtctcg tattcgaaga ccttcatgaa gcgcacgatg
      121 agtcgttggc attgttgcag tttttgctcg agcatctcga tggtcccgtt ttggctctgt
      181 gtattgctcg caacgaattg gaaacgcgtc gtgatggctg gcgttcgatc cgcaaggagc
      241 gacatcgagt catcgacctg gggccacttg gggaaacgga tgcggcaagc atgatgcagg
      301 cgttgttggc gccgtgcggg gaagctccac agccgttggt ggatgcggcg tgccagcttg
      361 ctggaggcaa tccgttattg ctcgagcaga tggttcgcat ttttcatgat actggggtgt
      421 tggaggagga ggatgctctt tcggagacgc cggtgtggct cgttcatctc ggcaagctcg
      481 aaacggtacg tcttcctctc acggtggaag acgccgtgca ggctcgagtg gcggcattgg
      541 ctccggcatt gcggttgctg ctcgagcaag cggcggcggt agggtcggtg ttttggctcg
      601 gagccttgac cgttttggcg cgtcttgaca gcaccacgcc ggagctttgg tctttgcaag
      661 acgacgcgga tattcgcaag attcgcgagt tgcttcagga gttgatggat cgggacttcg
      721 tgttgcgttt gcctgattcg acgtttcctg gggatgaaga gtatgtattc aaacacaatc
      781 tggaacgaga gcgcgtcgcg aagctgacct cgccttcggt gtcccgtcgt tatcatcggt
      841 tgatcgcgga ctggctcgag catcagtcca acaccagatc ggatgaggag tacgtggcga
      901 tgcttgcatc gcatcgggag gctgcaggtt cgatgcggga ggctgcgatg gcctatctgg
      961 acgcggggga tgtggcccgt tcccggtatt ctcctgcgaa agcgatagaa cattatgcgc
     1021 gggggctaga gttgcttggg gatgacgaac cgagacgtcg tctcgaggtg tatcatcatc
     1081 aaggcgatgt ctgtcagcag ctcggaaaga tggaggaagc gcttgtttcg tttcgctcga
     1141 tgctttcatt ggcgtaccgg ttgaatctat ttggcaaagg cggggcggcc cacaatcgca
     1201 tagggcggct gcatcgagag acgggagtgt tggatcaggc ggctcgacat cttggaacgg
     1261 ggctcgcttt gttcgaagca tgcaacgacg agcgggggat tgcatcgagc ctcgatgaca
     1321 ttggaaaact tcattggctt cgtggtgatt accccttggc gctcgagcag atgcgtcgtg
     1381 ctctcaccat gcgtcgacag attggtgacc ggaggtcgat agcgctttct ttgaacaatg
     1441 tcggattggt gcttcaggat tctggcgaat tcaaggaggc cctcgagtcg ttttcgcaag
     1501 ctttgaagat acgaagagag attggggact tgcttggggt gattatcacc ttgaacaatc
     1561 tcggtacggt tgcgcaggac cagggcaatc atgcgagagc actggaggtg tttgaggagg
     1621 cgttggcggt agctcgcgag gttggggata gaaacaagat agcgctcgtg ttgacgaatc
     1681 tgggcgaggc gcagcgttcg atggggcagg cttcggaagc ggtgttgagt ttgaccgaag
     1741 cggtgacaac gtgcgaggat ctgaacgacc gtctaggggt agcggaagca ttgcgcgcgc
     1801 ttggaatggc ctatttggaa cagaacgaac tggtccaggc tcgtgagagt atttctcgtg
     1861 cggttgacat ctttgccgcc gccagaagca aggttcatct tggttcggcc ctccgcacgc
     1921 ttggggaaat caccgcgggg ggaggttggg gccgtgagca tgcgagcaaa gctcgtgatt
     1981 atttccaacg atccatcgcg atcttcgaag agattggaaa tgaattggag cttgctcgaa
     2041 gtttgacaag ctatgcggat tttctggatc gaggagaact ggtcgaccat attgtcgagg
     2101 actcacgtgt gttacgccat agggccgagg gaatcttttt aaaactaaga aactcatctc
     2161 ctacgaacca tgcaagctaa ttcagcccaa cccaacacgc tgcatcagcg gttgcagcag
     2221 gtcgcctctg tcatttccgg ccaattcgtt ggaaaagacg agatcattcg tttgcttttg
     2281 gtcgctgtga tcgcggggga acacgcggtg cttctcggcc ctccgggaac ggcaaaaagc
     2341 gcgctcattc ggtcgttgtc ccaattgctc caagcgcgct acttcgagta tttgctcacg
     2401 agattcacgg agcccaacga gattttcggt ccggtggata tcgcggcgtt tcgcgaaggt
     2461 agttatcggc gtcgaaccga ggggatgtta cccgaagcgg aaatcgtatt tctcgacgag
     2521 gttttcaagt ccaacagcgc cattctcaat gcgctgctga ctttgttgaa cgaaaggcgg
     2581 gtggtggtgg gcggacaagc gattccgtgc ccgctcatca gtgtatttgg tgcatccaac
     2641 gaggtgcctg gcgacgagac gctgaatgcg attttcgatc ggtttctcat ccgtgtgcat
     2701 tcggacaatc tggatgccta ccacttttcg gaactgcttt ctcgtggtgt ccagcatgaa
     2761 ctgcgcgcga tgaaacagca ggctttgcat cccttggtgt cgtcgacgga gttgatgtcg
     2821 ttgtcaacga tggtgggtca gcggatgcag ttcaacgagt catttttgtc ttcgtacaag
     2881 gggttggtat ttcagattcg ggccgaaggc attagtttga gcgatcggcg ggtggtcaag
     2941 cttctcaagc tatttgccgc aaacgcctat ctcgatgggc gccaagccgc ggacgcgagt
     3001 gattttttcg ttctcaagca tatttggaac aatgaagatc aggtgcctgt tcttgcaggg
     3061 atcgtgcagc cggtgttgga agcattttat cgtgagtatc ccgaccgcaa gcgagttggc
     3121 gccgcggggg ttg
//
LOCUS       DAOSOR010000654         3120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_31254_length_3120_cov_3.852529, whole genome shotgun sequence.
ACCESSION   DAOSOR010000654 DAOSOR010000000
VERSION     DAOSOR010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31254_length_3120_cov_3.852529"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1547)
                     /locus_tag="PK710_23160"
     CDS             complement(<1..1547)
                     /locus_tag="PK710_23160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOT12698.1"
                     /translation="MDANRAIRSPVIRNAFPHSQHSSFPFLFDRALVILAEIMASTSS
                     FLPSWPADLAAKGWDQLDILIISGDAYVDHPAFGPALVARWLEHHGYRVGMIAQPRWD
                     SIEDISRLGRPRLFVGVSAGNLDSMLNKLTAQKKVRSTDDYSPGGQPGLRPNRATVVY
                     SNLCRQAFPGLPIILGGIEASLRRLAHYDYWSDSVRRSVLFDAKAHLLVFGMAERAIV
                     QIAHRLNRGETVDQLTEIPGTAHPLGSPRQWKHLAEASSQRPGDGKPLLLPSFEEVRA
                     DKKAFARMARLAHAESSPYHARCLLQPHGDEAVWLNPPALPLKTEELDRLYRLPFCRA
                     QHPSYEKPVPALATVRHSLAVVRGCFGGCAFCSIAEHEGRIVQSRSPDSILEEVRLLV
                     KGKGFDGTLTDVGGPTANMYGLRCKNEALQRVCRRSSCLHPRVCRHLGTDAGPYLHVL
                     RALRKVPGVRHVLVQSGIRLDLAERSPAFVQELAQFHTGGQLSVAPEHCQSHVLQAMR
                     KPSIECY"
     gene            complement(2272..>3120)
                     /locus_tag="PK710_23165"
     CDS             complement(2272..>3120)
                     /locus_tag="PK710_23165"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOT12699.1"
                     /translation="DVGFHNLRRISQVVREERGKQGLVIGEINAHPARYLSVVDGVLQ
                     YYVLGLLLEWTRGGIGNARLGRLLQRMVDEVGIDALLRCWLMLENHDTPRLMDRIPDR
                     TQRHALALLTFSLPGCVCLYYGVELGMTGEKDPYNRAAMRWDLCTDTNEELQFYRKLV
                     ALRKEKRALRVGDFVRLDTEHVLAFLRRTRRWRETLVILANGSNTPVQEVVCLEDPML
                     MQYLELLPLFSIHERSMEPIVIGPAGTISITLAPHDVVILGLKLPPRFSGMSPSGAYE
                     VARRAR"
BASE COUNT          714 a          870 c          876 g          660 t
ORIGIN      
        1 tcgtagcact cgatcgatgg ttttcgcatc gcttgaagaa catgggattg acagtgttcc
       61 ggcgctacgg aaagctgtcc tcccgtatgg aattgcgcca actcctgaac gaaagcggga
      121 gaacgttccg ccaaatcgag gcgaatcccc gattggacca gcacgtgacg aactccgggc
      181 accttgcgaa gggctcgcaa tacatgcaaa tagggccccg cgtcggtccc caaatgacgg
      241 caaacacgcg ggtgaaggca ggaagaacga cggcatacac gttgtaacgc ctcgttttta
      301 caacgtaatc cgtacatatt ggccgtcggc ccccccacgt ccgtcagggt tccgtcgaat
      361 cccttgcctt tcaccagcaa gcgcacctct tcgaggatgc tatccggact acgactctgc
      421 acgatccgcc cttcatgctc cgcaatcgag caaaatgcac aaccgccgaa acaaccacgt
      481 acgactgcca aggaatgacg aaccgtcgcc agtgccggta ccggtttttc ataactagga
      541 tgttgtgcgc gacaaaacgg aagacggtaa agacggtcca gttcctcggt ttttagaggc
      601 aaggcgggcg gattgagcca taccgcttcg tcgccatggg gctgcaacag acagcgagca
      661 tgataaggag aggactccgc atgcgccagc cttgccatcc gtgcgaaggc ttttttatcc
      721 gcccttactt cttcaaagga aggtaataac aaaggttttc catcccccgg ccgttgactc
      781 gaagcttccg cgagatgttt ccactgcctg ggactcccga ggggatgggc cgtaccaggg
      841 atttccgtca actgatccac cgtttcgcct cgattcagac gatgagcgat ttgcacgatg
      901 gcccgttccg ccatcccaaa caccaaaaga tgggctttcg catcgaaaag cacggaacgc
      961 cgaacggaat ccgaccaata atcataatgg gccagacggc gaagagaagc ttcaattccc
     1021 cctaaaataa taggtaatcc aggaaaagcc tgacgacaaa gattggaata aacgaccgtg
     1081 gctcggttag ggcgaagtcc gggttgaccg cccggactgt aatcgtcggt cgaacgtact
     1141 tttttctggg cagtgagctt gttgagcatg gaatcgagat tccctgcact gacgcctacg
     1201 aatagccgcg gacgtcccaa ccgtgaaata tcctcgatcg aatcccatct aggttgtgcg
     1261 atcattccca cgcgataccc atggtgctcc aaccaacggg cgacgagggc gggtccaaag
     1321 gcggggtgat cgacgtaggc atcaccggaa atgataagga tatcaagctg atcccaacct
     1381 tttgccgcaa ggtccgccgg ccaactggga aggaacgatg aggtcgatgc catgatttcg
     1441 gcaaggatga caagggctcg gtcaaagaga aagggaaaag aggagtgttg agagtgggga
     1501 aaggcgttgc ggatgaccgg cgagcggatg gctcgattgg cgtccacctc ggtatttcgt
     1561 tgttctgcag ctcgggaggg cagaaaacca cgcgtattga tgctctcttc cgtttcaacg
     1621 tgcctgccgt ttcatttcag tctatcttat catgatgttt attcgtcagg tgctttgcgg
     1681 cagttgggga ctcgtgggga tcgtcggcag tatgctaagc tgttgtccac cccaagagaa
     1741 tggcgagaaa tcagtggcaa cgctcccttc ggcacgcgcg cctgagccgc gcgccacgcc
     1801 gtcaacctcc ccgacgtggc ccgtccgaga gtgtgaatca cctgctccct gtcgcatgcc
     1861 gaatgggcaa ccgggcatgc gctgctcgga aacggaagct tgtttcaacc cttgtcccgc
     1921 gggcatgggg cctgagaagg aaggtagttt ttgcgcgaaa atttgcaaaa cggcttccga
     1981 ttgcggtggc gaccgctgtt ccccggaagg aatatgcgac cgatggcctg ccccgttacc
     2041 gtgtcaagac cccgagtttt gcacggtttc gggtgattat ggcggatttc gatgctctcc
     2101 atccagtccg tgtcagagtc cttgtaagaa ggggcttgtg ctgtttggcg gaacccattg
     2161 cgcgaaaccg tgtgagaagg agagccaatg ccctggtggt cattgcgaaa acgggatttg
     2221 tgctccctta tgtccttcgg aaggatgccc ctaccgctgg gaatgaggtg tttatcgcgc
     2281 tcttcgcgcc acttcatacg cgccggaggg gctcatcccc gaaaaccggg ggggaagttt
     2341 gaggcccaat atcacgacgt catgaggcgc gagggtgatg gaaatggtgc ccgcggggcc
     2401 aatgacgatg ggctccatcg atcgttcatg aatggaaaag aggggaagga gttccagata
     2461 ttgcatgagc atcgggtctt cgagacacac cacttcttgc accggggtgt tgctgccatt
     2521 ggcaaggatc accagcgttt ctcgccacct tcgggttcgt cgcaggaaag cgagcacgtg
     2581 ttcggtgtcc aaacgtacga agtccccaac gcgtagagcc cgtttctctt ttcgaagcgc
     2641 taccagtttt cgataaaact ggagttcttc attcgtgtcg gtgcataaat cccatcgcat
     2701 ggccgcgcgg ttgtacgggt ctttctcgcc ggtcatccca agctctacac cgtagtagag
     2761 acagacacat ccgggtaatg agaaggtgag caacgcaagg gcatgccgct gtgtccggtc
     2821 gggtatccga tccatcagtc gaggcgtgtc gtggttttcc aacatcagcc agcaccgcag
     2881 caaggcatcg atcccgacct cgtccaccat gcgttgtagc aatcgcccca atcgcgcatt
     2941 gccaattccc ccgcgcgtcc attcgagcaa caatcccaat acgtaatact gaagtacccc
     3001 atcgacaacg gaaagatatc gagccggatg cgcgttgatc tcgccaatga ccagcccttg
     3061 ttttccccgt tcttcccgca cgacctgcga gatacgacga agattgtgaa agccaacgtc
//
LOCUS       DAOSOR010000655         3119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_31268_length_3119_cov_4.458225, whole genome shotgun sequence.
ACCESSION   DAOSOR010000655 DAOSOR010000000
VERSION     DAOSOR010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31268_length_3119_cov_4.458225"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PK710_23170"
     CDS             <1..773
                     /locus_tag="PK710_23170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HOT12700.1"
                     /translation="EKSLGYAVLWTSDGAETWSTRSQGHDLRFRRVYFISPTQGWAVG
                     TRGNEGVLAKTTDGAKTWTFAKAPAHPPFFNQEPAVTRCEDVRFFDDQRGVALCLCCT
                     GNCEPEEGQSPSYLTSLLRTYDGGQTWSMDMDYEAKMAVPPFPEMSKASGMLTMSFPD
                     PNHGFMAGQNNIILRYEASQPEAEAWPAASCESSSGTGGTGTGGVSQGSDTPSEDKQN
                     EKDEGGCGCRVAPSSRSPLTALLFGIALLCRRRRTNLS"
     gene            776..2263
                     /locus_tag="PK710_23175"
     CDS             776..2263
                     /locus_tag="PK710_23175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOT12701.1"
                     /translation="MKRLLDTARRRPRDVAIVGLSCLLILLLCCTMIVRITYPFDLEW
                     MEGAMVDHVHQVLEGKRLYGPPSLRFVPFIYPPVFTWLASSIALVLGPGYLALRLVSV
                     AATAATMACIYCFVRREGAGWVESLMATGLYAGCYPLSGYYYDVGRVDASSVCFMLWA
                     AFAVRFGRSVGSAMGAAALLIVAYHTKQTSILLALPLGAYLWWVERRRAFWFWGAWIA
                     LGLGTSVALDTVHQGWFGYYTHRVVGGHAIAWDQLLAVLRGEIVIPFALALGVACFPY
                     LSRSAIGRKHARLYYALLTIGLLVIAGLGRIHTGGWINVLMPIHAGVAVVTGWGLFAA
                     REHNESNPLILRFIYLLLGVQLIMMIWDPRPAIPTQADRQAGHAWLNHVRSIHGDVWT
                     THRGHLTFLAGKKRHAHMMAIQDVMRSTADFRGAKSRLAMEVNEALASDQFARMLMDN
                     DDFWFMPSVQQHMVRDGDHWFSQPDVFWPRAGARLRPEIGYVPKR"
     gene            complement(2341..2616)
                     /locus_tag="PK710_23180"
     CDS             complement(2341..2616)
                     /locus_tag="PK710_23180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12702.1"
                     /translation="MIGDDPHRPIDIISPSVDDANRKLKWGVGLGAFALASYAVFGTV
                     CPICLVATPALLGAGLFERVRNHRACARLDKPSDTVQATPSNRTPQQ"
     gene            complement(2659..>3119)
                     /locus_tag="PK710_23185"
     CDS             complement(2659..>3119)
                     /locus_tag="PK710_23185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12703.1"
                     /translation="ATATSRLPFPGTEEGAKALLEGFLKSDADRKTMTSSLRPDAADF
                     DAIFTSDVVANARAAYDEKWKEEPVVEAREGQTELQMWKATTEQLRDGSGDASEFPSG
                     YKKIADKLKPGLTFYRFKFVKPGDKHGNAFDGLVFVNGHWVMVSKPYRYL"
BASE COUNT          599 a          862 c          946 g          712 t
ORIGIN      
        1 aggaaaaatc gttgggatac gctgtgttat ggacttccga cggggcagag acatggtcga
       61 cgcgttcgca gggccacgac ctacgtttcc gtcgcgtata tttcatttcc cccacgcaag
      121 gctgggctgt gggaactcgc ggaaatgagg gcgttcttgc gaagactacc gatggcgcca
      181 aaacgtggac gtttgccaaa gcccccgcac atcctccgtt tttcaatcag gagccagcgg
      241 tgacgcgatg cgaggacgtg cggtttttcg acgatcaacg cggagtcgcc ctgtgcctgt
      301 gctgtacggg caactgcgag ccggaggaag gccagagtcc gtcgtacctc acatccttgt
      361 tacgaaccta cgacggcgga caaacctggt cgatggatat ggattacgaa gcgaagatgg
      421 cggtgccgcc gtttccggaa atgtccaaag catcgggaat gctcaccatg tcatttcccg
      481 atccgaatca tggattcatg gcgggacaga acaacatcat cttgcgctat gaagcaagcc
      541 agccagaagc tgaagcgtgg cccgcggcct cttgtgaatc atcgagtggc accggaggaa
      601 ccggaaccgg cggcgtttcc caaggctccg atacaccctc cgaagacaag caaaacgaaa
      661 aagacgaggg aggctgcggc tgtcgcgtcg ctcccagctc tcgctcaccc cttaccgccc
      721 tactattcgg aatcgcgctg ctttgtcgcc gtcgacgcac caacctgtcg tgaatatgaa
      781 acgactgctg gataccgcac gtcgtcgccc gagggacgtc gctatcgtcg gtctgtcttg
      841 cctgctcatc ctgttgttgt gttgcacgat gatcgtgcgc atcacctatc catttgatct
      901 cgaatggatg gagggggcga tggtggacca tgtgcatcaa gtgctcgaag gaaaacgcct
      961 ttacggccct ccctcccttc gattcgtgcc cttcatctat ccgcccgtct tcacctggct
     1021 cgcctcgtcg atcgcccttg tcctaggacc ggggtatctc gcgttgcgtc tcgtgtcggt
     1081 agcggccacc gcagccacga tggcctgtat ctattgtttc gttcgtcgtg aaggagcggg
     1141 ttgggtcgag tctttgatgg cgacggggtt gtatgcgggc tgctatccgc tatccggata
     1201 ctattacgac gtgggacggg tcgatgcctc gagcgtttgc ttcatgcttt gggcagcgtt
     1261 cgcggtgcgg ttcggacgaa gtgtcgggtc cgcgatgggc gcggcagctt tgttgattgt
     1321 cgcgtaccat acgaaacaaa catcgatctt gttggcgctt cccctgggcg cgtatctctg
     1381 gtgggtggag cgtcgacgcg ccttttggtt ctggggcgca tggatcgcct tgggcctggg
     1441 cacgagcgtg gcgttggaca cggttcatca aggatggttt ggctactaca cccatcgcgt
     1501 ggtgggcggt catgcgatcg cctgggatca gctccttgcc gtgttgcgcg gggagattgt
     1561 gatcccgttt gccctggcgc ttggagtggc ttgctttccc tacctttcgc gatcagcgat
     1621 cggaaggaag cacgcacggt tgtattacgc gttgctcacc atcggtttgc tggtcatcgc
     1681 gggtttgggg cgaattcata cgggcggatg gatcaacgtg ttgatgccca ttcacgcggg
     1741 agtggccgtg gtgaccggat ggggattgtt cgccgcacgg gagcataacg aatctaaccc
     1801 attgatttta cgttttattt atctgcttct gggcgtgcaa ctcatcatga tgatctggga
     1861 tccgaggccc gcgatcccca cccaggcgga tcgacaagcg ggtcatgcct ggctgaatca
     1921 cgtgcgttcg atacacggcg atgtgtggac gacccatcgc ggtcacttga cctttctagc
     1981 cggcaaaaag cgtcatgcgc acatgatggc catccaagac gtgatgcgtt ccacggcgga
     2041 ctttcgtggt gccaagtcac ggttggccat ggaagtgaac gaagccctcg catccgatca
     2101 atttgctcgg atgctgatgg acaacgacga cttttggttt atgcccagcg tgcaacaaca
     2161 catggttcgg gacggtgatc attggttttc gcagcctgat gtattttggc ctcgagcggg
     2221 tgcgaggctg cgcccggaga tcggctacgt tccaaaacgc taatcgtcgg tggaacacga
     2281 atccacaaaa cccacgataa cccccaagga ttgagaggag tcggcctacc cccacgaagg
     2341 ctattgttgg ggtgttcggt tcgaaggagt tgcttgtacg gtatcggacg gtttgtcgag
     2401 ccgagcgcaa gcgcggtgat ttcttacacg ctcgaaaagc cctgcgccaa gcaacgcggg
     2461 cgttgccacc aggcagatcg ggcaaacggt tccgaagacc gcatagcttg ccagggcgaa
     2521 agcgcccaag cccacgcccc acttgagctt gcgattggca tcgtcgacgg aagggctgat
     2581 tatgtcgatg gggcggtgag gatcgtcacc gatcatggtg ttggcagtat ggatccgaat
     2641 cgcggcagcg caagcagttc acagatagcg atagggtttg ctcaccatga cccaatgacc
     2701 gttcacgaat accaaaccat cgaaagcgtt gccgtgtttg tcgccgggct tgacgaactt
     2761 gaagcggtag aaggtgagcc cgggcttgag tttgtccgcg attttcttgt agccgctcgg
     2821 aaactcgctg gcatcccctg agccatcgcg aagctgctcg gtcgttgctt tccacatctg
     2881 caattccgtc tgtccttccc gcgcttccac cacaggctct tctttccact tctcatcgta
     2941 ggcagcacga gcgttggcga cgacatcgga ggtgaagatc gcgtcgaagt cggcggcgtc
     3001 gggacgcaaa gaactggtca tggtcttgcg gtcggcgtcg gatttcaaaa atccttcgag
     3061 caacgctttg gcgccttctt cggtccctgg aaacggcaag cgtgaggtcg cggtggcca
//
LOCUS       DAOSOR010000656         3118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_31280_length_3118_cov_4.311786, whole genome shotgun sequence.
ACCESSION   DAOSOR010000656 DAOSOR010000000
VERSION     DAOSOR010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31280_length_3118_cov_4.311786"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="PK710_23190"
     CDS             <1..1046
                     /locus_tag="PK710_23190"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOT12704.1"
                     /translation="MGLVAGLTGSLFGAALSNRWVIIGIALVFLALAGSMFGLFEFVL
                     PSKLTNKLATMGGVGYGGAFVLGLISGVVAAPCTGPVLTGILLWIGKTQNPWLGAAAL
                     FAFSLGLGVPFWLVGTFAVRLPKSGKWMVAIKSFFGIVLAVVALYLVKNAFPQLTASL
                     PSAVSVPLVAGALAVVGIALGAIHLNFDEGPGRAVRKTIGLVLTIGGLAFLLAWLEKP
                     KEFSDQDTSSSDGGEAFDWKTDETEALAAAKAGNKPMLVDFTAEWCSACKRLARETFP
                     ESTVRAELGRFVLLKIDATQDDDPKVMELQKKYRVVGLPTVILLDAKGNEVERLTDFV
                     PPSVFINSLRRVK"
     gene            1367..2476
                     /locus_tag="PK710_23195"
     CDS             1367..2476
                     /locus_tag="PK710_23195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12705.1"
                     /translation="MNIFPIQRILALARWAPSGDNTQPWRFQVLGKDRLHILGHDTRA
                     HCVYDLDGHASQIAQGALLETLAIAATTEGLRADMARKTSSADSAPVYEITLTAHQDS
                     RPHPLAAMIEKRAVQRRAMSSRPLSEAHKDALSSSVTGYRILWFEGLAQRWNMARFMA
                     ANAKNRLTMPEAYPTHRDIIEWNAQFSDDRVPDRAVGVDPLTTRLMRWALQRWERVRF
                     LNTWLGGTWVPRLQLDLIPGLRCAAHFALVAEQEPSTIDDFVMAGRSLQRLWLTATSL
                     GLVLQPEMTPVIFSRYIRQGRNFTSVPAIHDRAARLAKRLERQLGTSMARHTVAMGRL
                     GYGPLPSSRSLRLPLCRLLVASDQTSQLAAEAQNQ"
     gene            2624..>3118
                     /locus_tag="PK710_23200"
     CDS             2624..>3118
                     /locus_tag="PK710_23200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12706.1"
                     /translation="MPVNLRVDPMSIGRWPRPWVACVLCLVGVVSPCEAQQQEMRPIR
                     VRLLWENRTQDPTCIDAKTLVKDVEDRLQRSVFIENNPDLVLEGAVVLEAGKPVVRIS
                     LHDANGSPIGRRELESEDRTCSTLNAAVPLSVALMIDYQQSTLRLHVPVVAVAPAPLA
                     AVARP"
BASE COUNT          622 a          964 c          853 g          679 t
ORIGIN      
        1 ccatgggctt ggtggctgga ctgacgggaa gtctgttcgg cgcggctttg tccaatcgat
       61 gggtcatcat cggcatcgcc ttggtttttc tcgcgctcgc cggctcgatg ttcggccttt
      121 tcgaattcgt tctcccttcc aaactcacca acaaactcgc caccatggga ggcgttggct
      181 atggcggcgc cttcgtgctg gggctcatca gcggcgtggt ggcagctccc tgtaccggcc
      241 ccgttctcac aggcatcctt ttgtggatcg gaaaaaccca gaacccgtgg ctcggtgcgg
      301 ccgctctttt cgctttctct ctcggacttg gtgttccttt ttggctcgtc ggaacgttcg
      361 ctgtccgcct ccccaaaagc ggaaaatgga tggtcgccat caaatcgttt ttcggcatcg
      421 tcctggccgt cgtggccctt tacctcgtca aaaatgcctt tccccagctc accgcctcgc
      481 tgccttccgc cgtgtccgtg ccgctagtgg ctggcgccct ggccgtcgtg ggcattgcct
      541 tgggggccat ccatctgaac ttcgacgaag gaccagggcg ggctgtccga aaaaccatag
      601 gcctggtgtt gaccatcggc gggctggcct ttttgctcgc ctggctggaa aaacctaagg
      661 aattcagcga tcaagacact tcttcctcgg acggtggcga agctttcgac tggaaaaccg
      721 acgaaacgga agctctcgcc gcggccaaag cgggtaacaa acccatgctg gtcgatttta
      781 cggcggagtg gtgcagcgcc tgcaaacgtc tggctcgcga aaccttcccg gaaagcacag
      841 tccgagccga actcggacgc ttcgtgttgc taaaaatcga tgctacccaa gacgatgacc
      901 ccaaagtgat ggaattacag aaaaaatatc gtgtggttgg tttgcccacc gtcatcctcc
      961 tcgatgccaa gggaaacgag gtggagcgtc tgacggactt cgtgccccca agcgttttca
     1021 tcaactcgct gcgtcgggtg aaataaagcc aaaaccactc ctccgccccg cctatcttca
     1081 tccttttcga acgaaacgat agaaaacgag gcaagctcct tcttggggcg gaagcgctcg
     1141 cctcctgcct caggccctgc ctgtctttcg acatcttctg aacaacaccc tcagaaaacc
     1201 caacccttca cggcacagct tactccaaag cccaccgtcg gctccaaggc gcgtcgagcg
     1261 aaggactcca ccatgaagct cggcccgcac gaggcttcgg agtccacatc gggcaaaggc
     1321 aaccaggcac aacgcaagcc atcccaccgt gtgaagaggc aaaatgatga acatatttcc
     1381 cattcagcgt atcctggctc tggcccgctg ggctcccagt ggtgacaata cgcaaccctg
     1441 gcgtttccag gtgctcggca aggaccggct ccacatactc ggtcacgata cgcgcgccca
     1501 ttgcgtgtac gatctcgacg gtcatgcgag tcagattgct caaggtgccc ttttggaaac
     1561 cttggccatc gctgccacga ccgaagggct tcgggctgac atggcgcgaa aaacgtcaag
     1621 tgcagatagc gcccccgtct acgaaataac cctgaccgcg catcaggatt cgcgccccca
     1681 tccgcttgct gcgatgatcg agaaaagagc cgtgcagcga agggccatgt cttctcgccc
     1741 tctatccgaa gcccataaag acgctctttc ctcgagtgtt accggctatc gcatcctgtg
     1801 gttcgaaggg cttgcccaac gctggaacat ggcgcgcttc atggccgcaa acgccaaaaa
     1861 ccgtctcacc atgccagaag cctaccctac gcatcgcgat atcattgaat ggaatgcaca
     1921 attcagtgat gaccgagtcc ccgatcgagc cgtcggtgtc gatccgctga ccactcggct
     1981 catgcgctgg gccttgcaac ggtgggaacg agttcgcttt ctcaacacct ggctcggggg
     2041 aacatgggtg ccgcgtctgc aactcgacct gatccctggt ttgcgctgcg ccgcccattt
     2101 cgccctggtt gctgaacaag agccctcgac catcgacgat ttcgtcatgg ccggtcgttc
     2161 cctgcagcga ttgtggctta cggccacgag tctggggctg gttttgcaac ccgagatgac
     2221 gcccgtgatc ttctcccgct acatacggca aggcagaaac ttcacatccg tgccggctat
     2281 ccacgaccgg gctgcccggt tggccaagcg cttggaaaga cagttaggaa cctcgatggc
     2341 tcgccacacg gttgccatgg ggcgccttgg ctatggcccg ttgccttcat cacgttcctt
     2401 gcgcctaccc ctttgtcgac tcctcgtcgc aagcgaccag acctcgcagc ttgccgctga
     2461 agcccagaac caatgaacct cggcagaacc ctttaaacat tcactaccgt ccccgattcg
     2521 ctgcactttt cgatgtggtt cctgccgttt gcacgaacaa cgacccatcg cgcacacaat
     2581 accctggata actgaatgcg ttctttgtag gaccggaagc acgatgcctg tcaacctgcg
     2641 tgtcgacccg atgtccatcg gccgctggcc cagaccgtgg gtcgcttgcg tcctttgtct
     2701 tgtcggtgtc gtttcccctt gcgaagccca acagcaggag atgcgcccga ttcgggtccg
     2761 cctgttgtgg gagaatcgca cgcaagaccc tacatgtatc gacgccaaga cgcttgtgaa
     2821 agatgtcgag gataggctac aacgtagtgt ttttattgaa aataaccccg atttggtctt
     2881 ggaaggggcg gtggtgttgg aagcgggaaa acctgtggtg cggatatcgc tccacgatgc
     2941 gaacggcagt ccgatcgggc ggcgcgaatt ggagagtgag gatcggacat gctcgacgtt
     3001 gaatgcggcg gtaccattgt ctgtggcgtt gatgatagac tatcaacaga gcacattgag
     3061 gctgcacgtt cctgtggtgg cggttgcgcc ggccccactg gctgctgtgg cgcgtccg
//
LOCUS       DAOSOR010000657         3118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_31281_length_3118_cov_3.792034, whole genome shotgun sequence.
ACCESSION   DAOSOR010000657 DAOSOR010000000
VERSION     DAOSOR010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31281_length_3118_cov_3.792034"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="PK710_23205"
     CDS             <1..1572
                     /locus_tag="PK710_23205"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT12707.1"
                     /translation="EKEALTLLRPAVDAIALAHRLGIAHRDLKPSNLILANTVQGSVL
                     KVLDFGIAKVMQEGETSALRATKTSSGFHAFSPQYGAPEQFLSSRFGATGPWTDVHAL
                     GLILTEIVVGKPAMVGEEHGDFHELAVRALRPTPRTLGAQVSDPFEALCRRCLASDPA
                     DRFPDADALLAAMDAIELGVRDWTVSTPVTPEHASETFPSPLEISDAALTIESTNHLP
                     FAETEASDAKTTPEGRRSRRLILGAGLAILFAVAGLVGWLVPSRSQNQAPTEEPCIAV
                     AGGTFRMGAEGAAPDQRPAHDVTVSDFALDKTQVTVQAYRLCVEAGACVAVDTSHLVS
                     NAEEQAKWNTFCNWGKPGRDQHPMNCVDWHQAQAYCAWVHKRLPSEQEWEYATKGGEE
                     QRMHAWGDDKPDADRLNACDLDCGQQAASVGWKWKPIYERSDGWPGTSPVGSFPNGQG
                     RWGHLDLAGNVWEWTSSPFCPYGDRRNEACSKTNMVARGGGWASRYPGIFRGSFRSKY
                     PREYRAADVGFRCAR"
     gene            2122..>3118
                     /locus_tag="PK710_23210"
     CDS             2122..>3118
                     /locus_tag="PK710_23210"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HOT12708.1"
                     /translation="MRSAQLLSLLSLTLLPVACGAEDPLPPPESTGQHTEPIIGGTLD
                     SDSSHDGVVMLYTQDGALCTGSVISQPGQKGVVLTARHCVSNVVSEYVTCKNDVSGDL
                     YPGSIYVLKGTSPNENSIIGRGEKLFHTGGTSLCDNDIAVMVLQDSLSGVQPLRVRKD
                     KQTFIGETFTAIGYGLTNPNSQYSAGKRYLRSNVQVTDFGPKYYGLYEKEFLGTVSIC
                     QGDSGGPAVSSDYAVMGVTSRGGDCNGNTNIWTRPEKFIDVIDQAVTYAGSTYQDDDG
                     NLHNGSGGGSGGSGGSGGSGGSGGSGGSGGTGGNGGTGGSSGVGGSGGDPVGGNGG"
BASE COUNT          699 a          829 c          963 g          627 t
ORIGIN      
        1 gagaaagaag ccttgacgtt gcttcgtccg gcggtggatg ccattgcgct ggcgcatcgt
       61 ctcggcatcg cccatcggga tctgaagcca tcgaatctca tcttggcgaa taccgtgcaa
      121 ggaagcgtac tgaaggtatt ggattttgga attgcgaagg tgatgcaaga aggagagacc
      181 tcggcacttc gagccacgaa gacatcgagt ggatttcatg cattttcacc gcaatacggt
      241 gcgcccgagc agtttttgag ttcgcgtttt ggggcgaccg ggccatggac ggacgtgcat
      301 gcgttagggt tgatattgac ggagatcgtg gtgggaaaac cagccatggt gggcgaagag
      361 cacggggact tccatgagct tgcggttcga gccctgcgcc ccacgccacg cacgttagga
      421 gcccaggtat ctgatccttt cgaagcattg tgccgtcgat gtctcgcaag cgatccggcg
      481 gaccgatttc ccgacgcgga cgctctatta gcagccatgg acgctatcga gttgggggtt
      541 cgtgattgga cggtatcgac gccggtgacg ccagagcacg cctctgaaac gttcccgagc
      601 ccgctggaga tttccgatgc ggcgcttacg atcgaatcga cgaaccatct tcctttcgcg
      661 gagacagaag cgtccgacgc gaagacaacg ccggagggac gtcggtctcg tcggttgatc
      721 cttggcgctg gtttggcgat attgtttgcg gtcgcgggtc tcgtgggctg gttggttccc
      781 tcccgttccc agaaccaagc gccaacggaa gagccgtgca ttgccgtggc agggggcaca
      841 tttcgcatgg gtgcggaggg cgcagccccg gaccagcgtc ccgcgcacga tgtcaccgtg
      901 tcggacttcg cgctcgataa aacccaggtc acggtgcaag cgtatcgatt atgtgtggaa
      961 gccggagcgt gcgttgccgt cgatacatct catctcgtat ccaacgcgga ggaacaagca
     1021 aagtggaaca cgttctgcaa ctggggcaaa ccagggcgtg atcaacatcc gatgaattgt
     1081 gtcgattggc atcaagccca agcgtattgc gcgtgggtcc acaagcgttt gccatcggag
     1141 caggaatggg aatatgcgac caaggggggg gaggagcaac gcatgcacgc atggggagat
     1201 gataaacccg atgcggatag gctcaatgca tgtgacttgg actgtggaca gcaggccgca
     1261 agcgttggat ggaagtggaa accgatatat gagcgaagcg atggctggcc gggaacttca
     1321 cctgttgggt cgtttcccaa cggccaaggg cgttggggtc atctggattt ggcgggcaat
     1381 gtatgggaat ggacgtcctc tccattttgt ccgtatgggg atagaaggaa cgaagcctgt
     1441 tcgaaaacca acatggtggc gcgcgggggg ggatgggcca gtcgataccc aggaatcttt
     1501 cgaggaagct tccgttccaa atacccacgg gagtatcgag cagccgacgt tggttttcgg
     1561 tgcgctcgat agatatcgct caggttgacc ggatcgggat gcacaaaaag caaccgcgca
     1621 aaggaaaggg ggggaggtat ccttcgcgcg gtctaagcgt gggaggatga ggggggtacc
     1681 ctctcacgcc ttcccaggaa accagcatag gacatgccaa atcaattact tgaaaactcc
     1741 agggcttgtt ggtcgatggg tgcgaagggg cttgcgacca tgcaatttca ccctttctca
     1801 acggcaacag cgactgtacg gaaatgtagt tcacggatca aacgctccct cagtaccagc
     1861 aaagtgaaga agtccgtttg gacgcgacga aaaatgtgcg ttccctgtcg atgaacaggt
     1921 gcgcttggcc gtcaaggaat gcacacttca ctctgtcatg ctctgtccca agccattgcc
     1981 cttaccacga tgaagattcg ccaccaccct tcggattggt acagatcctg cgcttgggta
     2041 cggaaagtct ccaaggtcgg ctgccccgcc gacaagcacc cgatgcaatc ccccacgcat
     2101 ggggtgcaga aagggaaatc gatgcgctca gcccagttat tatccctgct atcactcacc
     2161 ttattgcccg tcgcctgcgg tgcggaagac cccctccccc ctcccgaatc cacaggtcaa
     2221 cacaccgaac ccatcattgg cggcacgctc gactccgaca gttcccatga tggcgtcgtc
     2281 atgctctaca cccaagacgg tgcgctttgc accggttcgg tcatttctca acccggccaa
     2341 aaaggcgtcg tcctaaccgc aagacactgc gtttccaacg tcgtttccga gtatgtgacc
     2401 tgcaaaaacg atgtgtctgg agacctatac cccggttcca tctacgtgct gaaaggaacg
     2461 agtccgaacg aaaacagcat catcggccgg ggagaaaagc tatttcatac cggcggcacg
     2521 tcgttgtgcg acaacgacat cgccgtgatg gtccttcaag attccctcag cggagtgcag
     2581 cccctgcgag ttcgaaagga caaacaaact ttcatcggag agacgtttac tgccattggt
     2641 tatgggttga ccaaccccaa tagccagtat tccgcgggca aacgatacct gcgcagcaat
     2701 gtccaggtca ccgacttcgg acccaaatat tacggcctgt acgaaaaaga gttccttggg
     2761 accgtgtcga tttgccaagg ggactcgggt ggcccagccg tgtcgagtga ttacgccgtc
     2821 atgggcgtaa cctcccgtgg tggggactgc aatggcaata ccaacatctg gacccgcccg
     2881 gaaaagttta tcgatgtcat cgaccaagcg gtgacctacg ccggatcgac ttatcaagac
     2941 gacgatggca atttgcacaa tggttcgggt ggcggcagcg ggggaagtgg cggtagcggc
     3001 ggaagtggcg gtagcggcgg aagtggcggc agcgggggaa ctgggggcaa cggcggaact
     3061 gggggcagca gcggagttgg cggaagtggc ggtgatccgg tcggtgggaa cggaggtt
//
LOCUS       DAOSOR010000658         3115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_31317_length_3115_cov_4.068954, whole genome shotgun sequence.
ACCESSION   DAOSOR010000658 DAOSOR010000000
VERSION     DAOSOR010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31317_length_3115_cov_4.068954"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="PK710_23215"
     CDS             <1..894
                     /locus_tag="PK710_23215"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOT12709.1"
                     /translation="TGNKVVRVLAAGTKVTAVASTASNGFYKVSHNGTTGWAHGGYMT
                     IVSTPSGSSGVSGSMAVGTALETVADLNLRSGPSTSNKILKVMYTGSKVTTVAKDPQN
                     GFYKVDHAGTVGWAHGGYLRKAGSGSSGGSSGGSSGSSKVDGAISRAKAGVGFSYWWG
                     HARWLPGGPSSSTKGSCSGSCPSCKHSGNYGADCSGYVGKIWQVPSNNNDLTHDWHPY
                     STSTFVGSSSLWTTVSRSNVKKADAMVYNSGGSGHIFLYESGDGWGSMWAYECKGCSA
                     GCVRNLRTAGSAYKAIRRSGY"
     gene            1232..1798
                     /locus_tag="PK710_23220"
     CDS             1232..1798
                     /locus_tag="PK710_23220"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOT12710.1"
                     /translation="MTFLELCLLAIALSVDAFSVGAVVGLRHRSRRQVFRIAFHFGLF
                     QALFPWLSALASRLFLDFIDPWDHWIVFTVLGIVGLRMVWEALRGEEHRREAIDLTRG
                     FKLIGLSTAVSIDAAAAGLVITTSNTPLLPSVALIGTVTMLATTLAMLGARRLSSSLG
                     KRAEVMGGIVLMLLGTRVLLSGLGIIAP"
     gene            1836..2543
                     /locus_tag="PK710_23225"
     CDS             1836..2543
                     /locus_tag="PK710_23225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12711.1"
                     /translation="MGTTTGFGFRLVLAFATLGMVVGCESPKPSPSLEGQASVDKTEP
                     EPPFALPAAREVPLQRVGEWVDRARAKLPANEVSKIDAWLREAVRMPVEVRGESRKTL
                     SDTTPLTCGYQEKMSCDFSGTLSEKAKHFVASTLLCMDKDDAILQRSDWKIEPNTPRG
                     KVVKLPVDDGLLRQCWEKGGVSLRLEVVGMPCVVPFPRQVRCAGEYNTCLHRCKGEEP
                     CEQTCDGNRRSCLAVCK"
     gene            complement(2551..>3115)
                     /locus_tag="PK710_23230"
     CDS             complement(2551..>3115)
                     /locus_tag="PK710_23230"
                     /inference="COORDINATES: protein motif:HMM:NF018095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOT12712.1"
                     /translation="LRDSFRGYRRWTSIQLMLLRFSREIRMLNVRFLSASNASIRWIA
                     ILFVVVQTLFLSAYASAETKSVRVKTANFREAPSDGADVLFTADRYYPVRILARKSGW
                     AKVKDFEGEVAWIAERLLGPQKGIVVIVEKTVARDGPQADASAVFDAKWSESFAVKQI
                     REGWVLVESAQGLSGWLSQDVVWGIEP"
BASE COUNT          667 a          884 c          871 g          693 t
ORIGIN      
        1 accggaaaca aggtcgttcg cgtgcttgcc gcgggaacca aggtcacagc ggttgcttcg
       61 acggcctcca acgggtttta taaagtgagc cataacggca ccacgggttg ggcccacggt
      121 ggctacatga ccatcgtatc cactccatcc ggcagttctg gcgtgtcggg ctcgatggcc
      181 gtaggaacgg cattggaaac ggtagccgat ctcaacctgc gctctggtcc ttccacatcc
      241 aacaaaatat tgaaggtgat gtacacgggc tcgaaggtga cgacagtcgc caaagacccg
      301 caaaacggat tttataaagt agaccatgcg ggaaccgtcg ggtgggccca cggcggttat
      361 ctgcgaaaag caggatctgg ttcttcgggc gggtcttcgg gcgggtcttc gggctcctcc
      421 aaagtcgacg gtgccatcag ccgagccaaa gctggtgtcg gcttctctta ctggtgggga
      481 cacgcgcgat ggctccccgg tggccccagc tcatcgacca aaggttcctg ctccggctca
      541 tgcccaagct gcaaacactc cggtaactac ggcgcggact gctccggcta cgtgggcaaa
      601 atttggcaag taccgagcaa taacaacgac ttgacccacg actggcatcc gtactcgacc
      661 tccacattcg tcggttcctc cagcctgtgg accacggtgt ctcgaagcaa cgtgaaaaag
      721 gccgacgcca tggtgtacaa ctccgggggg tctggacata tcttcctgta cgaatcggga
      781 gatggctggg gctcgatgtg ggcttacgaa tgcaaaggct gctcggcagg atgcgtccgc
      841 aacctccgaa ccgcgggaag cgcctacaaa gccattcgcc gaagcggcta ctaatctccc
      901 ctttcgtgag tccccgccga gttttcccta cacttaccga ctttaccact gtgatatccc
      961 atcgctcata cgtgatcccc gagatccagg actgcgtttc cactaccacg ctcaagccca
     1021 ctttttctca cctgctcacc tacactgggg ctatcgagac cacataatcc ctgagcttgt
     1081 ttgcatccca actgtgatct ccgggatcaa gatgatcgcg agcatttttc gcgaccagcc
     1141 ccgccttttg cgcaagctga ggcaacgacg attcccaccc tcatcaatgc aaggttgcac
     1201 acaccgtgtg cctccaccat cgtgacggcc cgtgactttc ctcgaactat gtttgctcgc
     1261 catcgcatta tccgtcgacg cgttttccgt cggcgcggtg gtgggactac ggcatcgaag
     1321 tcggcgacag gtattccgca ttgcttttca tttcgggcta tttcaggcgc tatttccatg
     1381 gttgagcgcg ctggcgagcc gcctattcct cgacttcatc gatccttggg accattggat
     1441 cgtatttact gtcctcggga tcgtcggcct tcgcatggtg tgggaagcgc ttcgagggga
     1501 agaacaccgg cgtgaagcga tcgacctgac gcgaggattc aaactgatag ggttgtcgac
     1561 cgcggtgagt atcgatgcag cagccgctgg cttggtcatc accacgtcca acacgcctct
     1621 gctgccgtcc gtggctttga tcggtacagt gacgatgctt gccacgactc ttgccatgct
     1681 tggcgctcga cgactttctt cttctttggg caaacgcgcg gaagtcatgg gaggaatcgt
     1741 gttgatgctg ctcggcacgc gggtgttgct ctctggtctg ggaatcatcg ctccctgaac
     1801 gtacactggc gtccatcatc gtagggaagg cgatcatggg aacgacgaca ggatttgggt
     1861 ttcgattggt tttggcattt gcgacccttg ggatggtggt ggggtgcgaa tcaccaaaac
     1921 cttctccttc tctcgaaggg caagcgagtg tcgacaagac agaacctgag ccgccgtttg
     1981 cgcttcccgc tgctcgtgaa gtgccgcttc agcgtgtagg cgaatgggtg gatcgcgcga
     2041 gggccaaact gcctgccaac gaggtttcga aaatcgatgc atggttgcgt gaggccgtgc
     2101 gtatgcctgt ggaagtgcga ggggagtcca gaaaaacact gtccgacacg acacctctga
     2161 cgtgtggcta ccaagagaaa atgtcatgcg atttcagcgg gacgctctcc gaaaaggcga
     2221 agcatttcgt agcctcgact cttctgtgca tggacaagga tgacgccatc cttcagcgat
     2281 cggattggaa gatcgaaccg aatacaccgc gtggcaaggt ggtcaaactg ccggtcgacg
     2341 atgggctatt gcggcaatgc tgggaaaagg gaggggtgtc attgcggttg gaagtcgtgg
     2401 gaatgccatg cgtcgtgccg ttccctcgtc aggtgcgctg cgctggcgaa tacaacacgt
     2461 gtttgcatcg ctgcaaaggc gaggaaccat gcgaacagac atgcgatggg aatcgccgaa
     2521 gttgcctcgc tgtatgtaaa taattgtatt tcaaggctca attccccata cgacgtcctg
     2581 actcagccaa ccggataatc cctgcgcgga ctcgaccaga acccatcctt cacgaatctg
     2641 tttgactgcg aacgactcgc tccatttggc gtcgaaaacg gctgacgcat cggcttgtgg
     2701 tccatcacgc gccactgttt tctcaacgat gacgacaata cctttttgtg gtcccagcag
     2761 acgttctgca atccatgcga cttccccttc gaagtccttg accttggccc atccgctttt
     2821 tcgcgcgaga atgcgaacgg gataataacg gtccgcggta aacaggacat cggcaccatc
     2881 cgatggtgct tctcgaaaat tcgcggtttt gactcgaacg cttttcgttt ccgccgatgc
     2941 gtaggcggac agaaagagag tctgcacaac gacaaatagg atcgcgatcc atcgaatcga
     3001 ggcgttggaa gcggacaaga aacgaacgtt gagcatcctt atctcccgcg aaaatcgcag
     3061 cagcatgagt tggatcgaag tccatcgacg atatccacgg aagctatcac gcaac
//
LOCUS       DAOSOR010000659         3108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_31405_length_3108_cov_3.457583, whole genome shotgun sequence.
ACCESSION   DAOSOR010000659 DAOSOR010000000
VERSION     DAOSOR010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31405_length_3108_cov_3.457583"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /locus_tag="PK710_23235"
     CDS             complement(<1..1446)
                     /locus_tag="PK710_23235"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOT12713.1"
                     /translation="MASPSLEEKLRRLELLHRVGLSLSTEKNRERLLETILVEAKEAC
                     HADGGTLYLRTADDHLRFAIMRSDSLGIRLGGTTGKPIDLPSIPMFDAETGLPNHGNV
                     ASYAASLKRSINVGDAYDTQTFDFTGTKRFDAHHGYHSESFLTIPLLNHEDWVIGVLQ
                     LINARDPETGRIVQFSEEDQHLAESLAAQAGIALHNHVLLEEQRILLERFIKVLAAAI
                     DAKSPYTGGHCERVPVIAEMLIGALCEDSSETFADFDMTEEERYEFRIAAWLHDCGKV
                     VTPPHVMDKATKLEAIHDRIHMVRDRIEILRRDAELEAARKVMAGQLSWQQASTMAKE
                     KSAELDDILTFLERVNMGSEDLADSDVQRIQQIAELQYRKGGEWVPLLTADEVSNLSV
                     RRGTLTESERIVINGHMVQTISMLESLPFPRMLKRVPEYAGGHHERMDAKGYPRGIHA
                     GTMSLPARVMAVADVFEALTARDRPYKRGKPL"
     gene            complement(1457..2785)
                     /locus_tag="PK710_23240"
     CDS             complement(1457..2785)
                     /locus_tag="PK710_23240"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF006559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOT12714.1"
                     /translation="MKAEFDLVLRDAIVMTPNGREAIDIGIRGERIAGLGSLAEESSE
                     KTLRLPGLTVLPGVIDSHVHFREPGFIHKEDIETGTQAAVLGGVTTVLEMPNTSPPTT
                     SAGDLQDKLARAHGRAYTDHAFFVGATVDNIEELFKLEQLRGCAGVKVFMGSSTGSLL
                     VEDDATLERVLMATQRRVVVHAEHELRLKARKRKAESMGHPRAHPEWRDWETALTATK
                     RLLAIASKTGRCVHVLHVSTAEELEVLSRHRDLATVEVTPQHLTLRAPECYEKHGTFA
                     QMNPPLRDGKHGDALWKAVREGVVDVIGSDHAPHTIDEKKRPYPSSPSGIPGVQTLVP
                     LMLDHVNAGRLTLERFVDLTSAGPARVFGISGKGRIAVGYDADLTIVDMNKKQRIEQN
                     WLASRCGWSPFEGKIVQGWPRVTIVRGRVVMRDNQIHVPIGRSARFCGTL"
     gene            complement(2811..>3108)
                     /locus_tag="PK710_23245"
     CDS             complement(2811..>3108)
                     /locus_tag="PK710_23245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridylate synthase"
                     /protein_id="HOT12715.1"
                     /translation="EAVPQTGRLHQIRRHFKHCGHPLVGDVRYGRGEINRLFRQEHGL
                     VRLALHAMELRFPHPCLGEMVSVTSALPHDLSTTLERLGVPAFLWKTPLMENVT"
BASE COUNT          673 a          972 c          769 g          694 t
ORIGIN      
        1 caggggtttt ccgcgtttat agggacggtc ccgggcagtc agagcctcga atacatccgc
       61 caccgccatc acacgcgccg gcagcgacat cgttcccgcg tggattccac ggggatagcc
      121 tttggcatcc atcctttcat gatgcccgcc cgcgtactcc ggcacccgct tgagcatgcg
      181 cggaaatggc agcgattcca gcatgctgat ggtctgcacc atgtggccgt tgatcacgat
      241 tcgctctgat tccgtaagcg tgcctcggcg cacggataag ttggaaactt catccgcggt
      301 caacaaagga acccattcgc ccccttttcg atactgaagc tcggcaatct gttgaatgcg
      361 ctgcacatcc gaatccgcca gatcctccga acccatattc actcgttcga gaaaggtgag
      421 gatgtcgtcg agttcagcgc ttttttcctt cgccatcgtc gatgcttgtt gccaggacaa
      481 ctgtcccgcc atgacttttc gcgcagcttc caattccgca tcacgacgaa ggatctcgat
      541 gcggtctcgc accatgtgta tgcggtcatg gatcgcctcc agcttggttg ccttatccat
      601 gacatggggc ggagtcacca cttttccaca atcatgaagc catgcggcga ttcgaaactc
      661 gtaccgctcc tcttcggtca tgtcgaagtc agcgaacgtt tcggagctgt cttcgcataa
      721 agcgccgatg agcatttccg caatcacggg cacacgttca caatgtccgc ccgtataagg
      781 tgatttcgcg tcgatcgccg cggccaggac cttgatgaat ctttcgagca gaatgcgctg
      841 ctcttcgagc aagacgtggt tatgcaacgc aatgcccgct tgcgccgcca aggactccgc
      901 aagatgttga tcttcctcag aaaattggac gatccgcccc gtttccggat cgcgtgcgtt
      961 gatgagttgc aacaccccga tcacccaatc ctcatggttc aacaacggaa tcgtcaaaaa
     1021 cgattccgag tgatacccat gatgcgcgtc aaaacgtttc gtcccggtaa aatcgaaggt
     1081 ctgcgtatca tacgcatcgc ctacgttgat cgatcgcttc agcgacgccg catacgaagc
     1141 gacatttccg tggtttggta agcccgtttc cgcatcgaac atcggaatcg acggcaagtc
     1201 gatgggctta cccgtggttc cccccaagcg aatgccaagc gaatcactac gcatgatcgc
     1261 gaaacgcagg tgatcgtccg cggtccgaag atagagcgtg cctccgtccg catggcaggc
     1321 ttccttggct tccacgagaa tcgtttcgag caagcgttcc cggttcttct ccgtcgataa
     1381 cgataagcca acgcgatgaa gcaactccaa tcgtcgtagc ttttcttcga gagaagggct
     1441 tgccatgatg gctccgtcaa agcgttccgc aaaaccttgc gcttcgtcct atgggaacat
     1501 ggatctgatt atcccgcatg acgactcgac ctctcacgat ggtaacgcga ggccaacctt
     1561 gcactatctt gccttcgaaa ggtgaccaac cgcaacggct tgcgagccaa ttttgctcga
     1621 tacgctgctt cttgttcatg tccacgatgg tcaagtcggc atcataacca accgcgattc
     1681 gcccttttcc cgaaatacca aacacccgcg ctggccccgc actcgtcaaa tctacgaaac
     1741 gttccaacgt caatctcccc gcattcacgt ggtcgagcat gagcggaaca agggtttgca
     1801 ctccaggaat ccccgaaggg ctagaaggat aaggacgttt tttttcgtcg atcgtgtggg
     1861 gagcgtgatc cgaaccgatc acgtccacca ccccctcacg aacagctttc catagtgcat
     1921 ccccatgctt gccgtcgcgc aggggtgggt tcatttgggc gaaagtcccg tgcttttcat
     1981 aacattcagg agcacgcaaa gtcagatgct gcggcgtcac ctccaccgtc gccaagtccc
     2041 gatggcgaga tagcacttcc aattcctccg cagtgctcac atgaagcaca tgaacacacc
     2101 gacccgtttt cgatgcgatc gcgagaagac gcttcgtagc cgtgagcgcg gtctcccaat
     2161 cacgccattc cggatgcgcc cggggatgac ccatggattc ggcttttcgt tttcgcgctt
     2221 tcaatcgcag ttcatgctcc gcatgcacca cgacacgacg ttgcgttgcc atcagcacac
     2281 gttccaacgt ggcatcgtct tcgacaagca atgaacccgt ggaacttccc atgaaaacct
     2341 tgacccccgc gcacccgcgc aattgctcca gcttgaacag ctcttcgata ttgtcgacgg
     2401 tggcgcctac gaaaaacgcg tgatcggtgt aggcacgccc atgcgcgcga gccagcttgt
     2461 cttgcaaatc ccccgcgctc gtcgtcggag gactcgtgtt cggcatctcc aagaccgtcg
     2521 ttaccccccc aagcaccgcg gcctgggtcc ccgtttcgat gtcttcctta tgaatgaacc
     2581 cgggttcccg aaagtgtacg tgcgaatcga tgactcccgg aagtaccgta agcccaggaa
     2641 gtcgcaacgt tttctcactc gactcctctg ccagactgcc caacccggct atcctctccc
     2701 cacgaatgcc aatgtcgatt gcctcccgcc cattgggggt catcacgatg gcgtctcgaa
     2761 gaaccagatc gaattcggct ttcattcctt ggctccaagc gcagtcggcc tcaggtcacg
     2821 ttctccatca aaggggtttt ccataaaaaa gcagggactc caagacgttc caaggtcgtg
     2881 gatagatcgt ggggaagcgc cgaagtcacg gacaccatct cccccaaaca gggatgagga
     2941 aacctaagct ccatggcatg caaagcaagc ctaaccagcc catgctcttg tcgaaatagc
     3001 cggttgattt cgcctcgtcc gtagcgcacg tcccccacca atggatgtcc acaatgcttg
     3061 aaatgcctgc gaatctgatg cagtcgtccc gtctggggca ccgcttcc
//
LOCUS       DAOSOR010000660         3098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_31525_length_3098_cov_4.455143, whole genome shotgun sequence.
ACCESSION   DAOSOR010000660 DAOSOR010000000
VERSION     DAOSOR010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31525_length_3098_cov_4.455143"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3084)
                     /locus_tag="PK710_23250"
     CDS             complement(<1..3084)
                     /locus_tag="PK710_23250"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOT12716.1"
                     /translation="MQGWNVPRKAGWDTHGLPVEVEVSKELRIPDKAGIEAYGIEPFT
                     QKCIESVFRYTSEWETLTEKIGFWVDLEQAYVTYHKSYVESVWWALSNLFDKGLLYQG
                     HKVVWWWAQGGTALSSHEVGQGWKTVDDPSVFVRLPLRDDPSTSLVVWTTTPWTLPSN
                     VYVAVDLSFDYVIVRDGEHKLIVAAELRERLETSIKRPLEVLETKKGKDLVGLSYIPP
                     FDIFYSEYKDLEAKGKDGTTRLAFWRVEPASFVTLDTGTGLVHVAPAFGEDDHALHRS
                     LLDTYENPSSIPLLCAIEPDGKFIDAMGEGVAKRWVKDADADLIKALDRRGLLVHAET
                     YRHDYPFCWRSDDDPLIQMARPAWYIRTTREIDKAKANNRNIHWIPDHIKEGRFGDFL
                     EHNVDWALSRERYWGTPLNVWVCSQDPEHKHAPDSVAAIEKLNPHAFEAFHAAKQKDP
                     SLSDHLMVHKPWVDNVVFPCPSCGAPMHRVPEVIDCWFDSGCMPFAQWGFPHVPGSLA
                     RFDHSFPADFISEAIDQTRGWFYSLLMVSTLVFDESTQKQMGLTRVRPVPHPYKACIV
                     LGHTCDKDGKKESKSKGNYTPPEVILDRVCMDFAVIDDAASRGLQSKEGEAWIATADL
                     EGLDLTDGATVSVSGSSDEARPFVLRGSRKLPRRVVLLHEADRNALGVEVGPLGANVM
                     VVEVPRLDRKYRVTIEDTQTPSPGADAFRWFFYASSPPWTNTRHSLSNVRSAQKEFQI
                     KLRNVYSFFTIYANIDGFDPSEGIAEWQGLDAAALASGKAVSAIKDRPLLDRWMLSEL
                     ALTVRDVTSHLEAYRVHESALRLIDCVDALSNWYVRRSRERFWAAGFSQDKRDAYWTL
                     YTSLTTLARLCAPFIPFFAEEIYQNLVRRPWPASQPLSVHLCQWPELDEKAVDEALSV
                     EMKAVRDIVSLGLQVRTNNRLKVRQPLRSVDVVLANRALKDRMKAYESLIRSELNVHE
                     VHWLDPGQEGKEVVYKLKPNFRALGPKLGKRVQEAKTALAKADAGELRTRLVTDGK"
BASE COUNT          689 a          915 c          826 g          668 t
ORIGIN      
        1 ttttccgtcg gtgacgagcc tggtgcgaag ctcacccgca tcggccttgg ccagcgcggt
       61 tttggcttct tgcacacgtt taccgagctt tggccccagt gcccggaagt tgggcttgag
      121 tttgtacacc acctctttgc cctcttgtcc cggatcgagc caatggacct catggacgtt
      181 gagttcgcta cgaatcaggg attcatacgc cttcattcgg tctttcaacg cgcgattcgc
      241 caaaaccaca tccacggagc gaaggggctg acgaactttc aaacggttat tggttcgcac
      301 ttgcaacccc agcgatacga tatcccgcac cgctttcatt tcgacggata aggcttcatc
      361 gaccgctttt tcgtccaatt cgggccattg gcagaggtga acactgagag gttgcgacgc
      421 gggccagggc cgtcgcacga ggttttgata gatttcctcg gcaaaaaacg gaataaaggg
      481 cgcgcacaac cgggccaacg tcgtcagact cgtatagagc gtccagtagg catcgcgttt
      541 gtcttgcgaa aaaccagccg cccagaaccg ctcacgactc cttcgcacat accaattgga
      601 caatgcatcg acacaatcaa tcaaacgtag cgccgattca tgaacccggt aagcctccag
      661 atgggatgtg acatcgcgca cggtaagtgc caattcgcta agcatccatc ggtcgagcaa
      721 gggacgatct ttgatggcac tgacagcttt tccggatgcc aacgctgccg cgtccagccc
      781 ttgccactcc gcgatcccct cggaaggatc gaagccgtcg atattggcgt agatggtgaa
      841 aaacgaatag acattgcgca gcttgatttg gaattctttt tgggcgctcc gaacgttgga
      901 cagggagtgg cgcgtattcg tccacggggg tgaagaggca taaaagaacc agcgaaaagc
      961 atcggccccc ggcgatgggg tttgcgtatc ttcgatggtg acacgatact ttcggtcgag
     1021 ccgcggcacc tccaccacca tcacgttggc acccagaggg cctacctcga cgcccaacgc
     1081 atttcgatcc gcctcgtgca gcaacaccac gcgccgcgga agtttgcgag atccccgcaa
     1141 gacaaagggg cgcgcctcgt cggaagaacc cgatacgctc accgtggcgc catcggtcaa
     1201 gtcgagccct tcgagatccg ccgtggcgat ccatgcctca ccttctttgg actggagccc
     1261 tcgactggcg gcatcgtcga tgaccgcgaa atccatgcaa accctgtcca atatcacctc
     1321 gggtggggtg taattgcctt tggacttgga ctcttttttc ccatccttgt cacaggtatg
     1381 accaagcacg atacaagctt tgtacgggtg ggggacgggc cgaacccgcg tcaatcccat
     1441 ctgtttttgc gtcgattcat cgaatacgag cgtcgatacc atcaacaagg aatagaacca
     1501 tcctcgcgtc tgatcgatcg cttcactgat gaaatccgcg ggaaaactat ggtcaaaacg
     1561 ggcgagcgac ccgggcacgt gaggaaatcc ccattgcgca aaaggcatgc accccgagtc
     1621 gaaccaacaa tcgatgacct ccgggacccg gtgcatcgga gcaccacaac tcgggcaggg
     1681 aaatacaacg ttatccaccc acggtttatg aaccatcaga tggtcgctca gcgatgggtc
     1741 tttctgcttg gccgcatgaa acgcttcgaa cgcatgagga ttgagctttt cgatcgccgc
     1801 gacactgtct ggcgcatgct tatgctccgg atcttgcgag catacccaaa cgttcaaagg
     1861 ggttccccaa tagcgctccc gcgaaagcgc ccaatcgaca ttgtgttcga gaaagtcacc
     1921 aaaccgcccc tctttgatgt gatccggaat ccaatggatg ttccggttgt tggctttcgc
     1981 cttgtcaatc tcgcgggtcg tgcggatata ccaggcagga cgcgccatct gaatgagcgg
     2041 atcatcgtcg ctacgccaac agaacggata atcatgacga taggtctccg catgaacgag
     2101 caggccccgc ctgtccaggg ccttgatcaa atccgcatcc gcgtccttca cccagcgttt
     2161 cgccacaccc tcgcccatcg catcgatgaa ttttccgtcg ggttcgatgg cgcaaagcaa
     2221 cggaatactc gacggattct cgtaggtatc gagaagggat cgatgcagcg cgtggtcgtc
     2281 ttctccaaac gcgggagcga catgcaccag ccccgtaccc gtatcgaggg tgacgaagga
     2341 ggcgggctcc acacgccaga acgcgagccg cgtcgtaccg tccttgccct tcgcctctaa
     2401 gtccttgtat tcactataaa aaatgtcgaa tggggggata taactcaggc ccaccagatc
     2461 ctttcctttt ttcgtttcga gcacttcgag aggacgtttg atcgacgttt ccaggcgttc
     2521 gcgcagttcg gcggcgacga tgagcttgtg ctccccatct cgtacgatca cgtaatcgaa
     2581 agacaaatcc acggctacgt agacattgga gggcaacgtc cagggcgtgg tggtccaaac
     2641 caccagactg gtggaggggt cgtcgcgcaa gggcaagcgc acgaaaacgc tcgggtcatc
     2701 gacggttttc caaccttgtc cgacctcgtg actcgacagg gccgtgcctc cctgggccca
     2761 ccaccagacc actttatgcc cctggtagag caaccctttg tcgaaaaggt tggacaaagc
     2821 ccaccacacg ctttcgacat agcttttgtg ataggtgacg taggcctgtt cgagatcgac
     2881 ccaaaagccg attttttcgg tcaacgtctc ccattcggac gtatagcgaa agaccgattc
     2941 gatgcatttt tgggtgaacg gctcgatgcc atacgcttcg atgcccgctt tgtcggggat
     3001 acgaagttct ttggacactt cgacttccac gggcaagcca tgggtatccc aaccggcttt
     3061 gcgcggcacg ttccagccct gcatggtttt gaatcgcg
//
LOCUS       DAOSOR010000661         3077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_31783_length_3077_cov_3.577432, whole genome shotgun sequence.
ACCESSION   DAOSOR010000661 DAOSOR010000000
VERSION     DAOSOR010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31783_length_3077_cov_3.577432"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..607)
                     /gene="crcB"
                     /locus_tag="PK710_23255"
     CDS             complement(209..607)
                     /gene="crcB"
                     /locus_tag="PK710_23255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOT12717.1"
                     /translation="MNSWREWLWIALAGAFGACARVGLSLFVHQHFGNALAWGTMSVN
                     VLGSFAFGVIVQIGLSSNWLPDELRLALTMGFLGAFTTFSTFSYETYRYAESGLWCSA
                     LANMMGNLVPGLLAVWLGLVLGRWIVAWGS"
     gene            complement(681..>3077)
                     /locus_tag="PK710_23260"
     CDS             complement(681..>3077)
                     /locus_tag="PK710_23260"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOT12718.1"
                     /translation="LSNGTWGFQRGTEKFEGCAECRGLREDLFTSELTHRYLIPMLIR
                     AGARVYTPREWDPRVDEIIVDDSGPGYQEQGSGWENGTSPGGHKGGYRVLAPGAAGSA
                     SFIPDLPNEGNYWISLRYVAGSNRSTATRVVIEHAGGSQEMVIDQRQDNSRWIYLGQF
                     WMRPGANHGVRLEPTAGDPGYVIADAVRFGGGIHAETSAPRWHMSAQAHLAYLGAPSS
                     ITSSGNDVTMRPLYAEWQGADVYLSIHSNATGSVNTTSGTSTYRYNCGTYSDHSTAPS
                     PSACDDPPRSDALQRAVHDGVIQMLREHWDPNWRDFGRRVANFGEVRALDQIPGALIE
                     LAFHDNTVTPSGSNPPRMPDNRALQEPRWRRLTARGLYKGLLEFLNPGAKMAPEAPVI
                     TSAVTEPAASGVGVRVRWEPVKDAKGYRVYVARGGKGFDGSTIVDSTDVLLDAAPGQP
                     VFVRVSALNEGGESLASEVLGAASQSGLADHKQVLIVQGFDREDAWTAEGGNTFDYVF
                     AHGLAIAAAGWGFHSATNEAVADGAIPLEPYTIVDWMLGEESVADATLDATERDKVEA
                     YTKAGGKLLVSGAEIGWDLVNTGKHEAFVQNVFGAAFEKDDSENDSLVAPSAGVLGSL
                     PEMHLDDGTQGTYPVEYPDVFLPSATGASQALTYGNGDGAAVAYDNGETRTFLAGFPL
                     ETVQPVAARDALMASILSFLVPEGPPEPMIPDAGVEDDAQTEPQEEAGTTADSGKKDA
                     AVDEGGVEVTMPPWSHDDSADGCACRASGMGSPHSAAWLWVGLGLLAAMMRNNRMRRA
                     "
BASE COUNT          655 a          931 c          892 g          599 t
ORIGIN      
        1 tgagtttacg tgcgtgcagg agtcgatagg gtgtaatttc ccgcagtagt attaagcccc
       61 cctttggacc accgccaaca gttgctttgg gctatcatac aatggattgg cgtactgcac
      121 tgaacattag gggatgaccg tgaccggttt gcgtgaaccg tgaccgtaaa agaaaggcac
      181 aaaaatgagg ggatagctcc gttccgcgtc aggatcccca agcgacgatc caacgcccca
      241 aaaccaaccc gagccagacg gcaagcagtc cgggaacgag attgcccatc atgttggcca
      301 atgccgaaca ccatagtccg ctttccgcat aacgataggt ttcatagctg aacgtggaaa
      361 acgtggtgaa ggcaccgaga aaacccatgg tcaacgcaag ccgaagttcg tcaggtagcc
      421 agttcgagga tagtccgatc tgcacgatca ccccaaaagc gaaggacccc agcacgttga
      481 cgctcatcgt cccccaggca agcgcatttc cgaaatgctg atgcacgaac aaggacaaac
      541 ccacgcgagc gcaagcgcca aaggctcccg ccagggcgat ccaaagccat tcacgccagg
      601 aattcattca tgtcaccggg tcggaaaagc ataagcctgt ccgctattgt ttcatgcggg
      661 aaggggagga taatcgtcga ttacgcccgg cgcatccgat tgttgcgcat catcgcggcc
      721 aaaagaccaa ggcctaccca gagccacgcg gcggagtggg gcgaacccat cccagacgcc
      781 cggcaagcgc agccgtcagc ggaatcatcg tgactccatg gcggcatggt cacttccacc
      841 cctccttcgt cgaccgcagc gtcctttttc cccgaatccg ccgtggtacc cgcttcttct
      901 tgcggttccg tctgggcatc gtcctccact cccgcatccg gaatcatggg ttccggaggt
      961 ccctcgggca cgagaaagga gagaatcgaa gccatcagcg catcgcgggc agcgacgggt
     1021 tgcacggttt cgagtggaaa tcctgccaag aacgtacgcg tttccccgtt atcataggca
     1081 acggccgcgc catcgccatt gccataggtg agagcctggg aggctcccgt cgccgatggc
     1141 aggaaaacat ctggatattc aacaggatag gtcccctggg tgccatcatc gagatgcatc
     1201 tcgggcaagc tgcccaacac tcccgcggaa ggagccacga gggaatcgtt ttccgaatcg
     1261 tctttttcga acgcggctcc gaagacgttc tggacgaatg cctcgtgttt gccggtattc
     1321 acgagatccc aaccgatttc cgcgccagag acgaggagtt ttcctcccgc cttcgtatag
     1381 gcttccacct tgtcgcgttc tgtggcatcc aacgtggcat cggcgacgga ttcttcgcca
     1441 agcatccaat cgacaatcgt ataaggctcg agggggatgg caccgtcggc cacggcttca
     1501 ttggtggcgc tgtggaatcc ccacccggcg gcggcaatgg caagcccgtg ggcgaaaacg
     1561 taatcgaagg tattgccgcc ttccgcggtc caggcgtctt cacggtcaaa gccttgcacg
     1621 atgagcactt gtttgtggtc cgcaaggccg ctttggcttg ccgcccccag cacctcggaa
     1681 gcaagagact ctccgccctc attcagagcc gatactcgca cgaatacggg ctgaccaggc
     1741 gccgcatcga gaagaacgtc ggtgctatcc acgatcgtcg aaccatcgaa tcctttgcct
     1801 ccccgcgcca catacacccg atagcctttg gcatctttca ccggctccca acgaacgcgc
     1861 acccccacgc cagaagccgc cggttcggtg accgccgagg tgatgaccgg cgcctcggga
     1921 gccatcttcg cgccaggatt gaggaattcg agcaagccct tgtacaatcc cctggcggtg
     1981 agccttcgcc atctgggttc ttgcaaggcg cgattatccg gcattcgcgg gggattgctt
     2041 cccgaaggcg ttaccgtatt gtcgtgaaat gccaattcga tgagggcgcc agggatttga
     2101 tcgagcgccc gcacctcgcc gaagttggcc acacgacgac cgaaatctcg ccaattcggg
     2161 tcccaatgtt cgcgaagcat ctgaatgacg ccatcgtgga ccgcacgctg caacgcgtcg
     2221 cttcgcgggg gatcatcaca ggcggaaggg ctcggcgcgg tactgtgatc actgtacgtg
     2281 ccgcagttat accgatacgt actcgttcct gacgtggtat tgacgctacc ggtagcgttg
     2341 ctgtgaatgg aaaggtatac gtcggctcct tgccattcgg cgtacagggg ccgcatggtg
     2401 acatcgttgc cggaagaggt aatggaagaa ggcgcgccga gataggccag atgggcttgc
     2461 gcgctcatgt gccatcgcgg cgcggaggtt tcggcgtgaa tcccgccacc gaaacgtacg
     2521 gcgtcggcaa tgacgtagcc agggtcgcca gcggtgggct cgagacgcac gccgtggttt
     2581 gctcctggac gcatccagaa ctggcccagg tagatccagc ggctgttgtc ttgccgttgg
     2641 tcgatgacca tttcttggga accgccagcg tgttcgatca cgacccgggt ggccgtggaa
     2701 cggttggaac cagcgacata tctcaatgaa atccaatagt tgccttcatt cggcaaatcg
     2761 gggatgaagg aagcggagcc cgcggctccc ggagctaaaa cgcggtatcc gcccttgtgg
     2821 ccccctgggc tcgtgccatt ttcccaaccc gagccctgct cctggtagcc gggaccactg
     2881 tcgtcgacga tgatttcgtc cacgcggggg tcccattccc ggggcgtata gacgcgagcg
     2941 ccggcacgaa tgagcatggg gatgaggtat cggtgggtta gttcggaggt aaaaaggtct
     3001 tctcgcaagc cgcggcattc cgcacaaccc tcgaactttt ccgttccgcg ttgaaaaccc
     3061 caggtaccgt tcgagag
//
LOCUS       DAOSOR010000662         3067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_31914_length_3067_cov_3.642762, whole genome shotgun sequence.
ACCESSION   DAOSOR010000662 DAOSOR010000000
VERSION     DAOSOR010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31914_length_3067_cov_3.642762"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1125
                     /locus_tag="PK710_23265"
     CDS             82..1125
                     /locus_tag="PK710_23265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12719.1"
                     /translation="MSEQDAPSPRRSACAVWTGTELFVWGGTAFNNLPLGDGGAYDPI
                     TDSWRPININGAPSARGEPNCVAMGTDVFVWGGSTNSGLVDGGAIWSSTQNTWKAIDS
                     HKAPKSRRWPAVVWTGERVLVWGGQTDDGKCEQSGSQYDPVLNTWNSMSDSSSPKHCK
                     RQAYAWMDTLLYVYGGRNDNDIAGNKLHSYDPATDVWKEVNTITLAARSNSFITPSDS
                     RLLVWSGFTTKEYGVPDGFVFDPSTCTWKPLTSPQPSSIRGRVPFRSGWCVWTGDAML
                     LVGGVKEGKIITNSITYNPNTKPQDQWSKAIPWDPPLEYDQGIGVWTGTEFILWGGSD
                     GSKPILGGSRWMP"
     gene            1697..2074
                     /locus_tag="PK710_23270"
     CDS             1697..2074
                     /locus_tag="PK710_23270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12720.1"
                     /translation="MADGQGAEDAEQSEEPAAVVSVGQNRSTTAMVLTIGGAGVFATG
                     LGMGLWGYVKAKDADSGEGEDADAARRLALIGDIGMGVGLVAAGIGTYLLFSEEDSTD
                     SSKVRVGPWAAAGAGGLVAVGRF"
     gene            complement(2466..>3067)
                     /locus_tag="PK710_23275"
     CDS             complement(2466..>3067)
                     /locus_tag="PK710_23275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12721.1"
                     /translation="RTGLSISQASWTIGRSPAVQSGRAIEVATMYAVEGTIVDPIANR
                     PIENISVIARPSTMSTGSYAERIFGTAPLIARAASTLTGAGGGFVLGVDPGVVDITAQ
                     PSDISRFPWLVVGRAKVPSEPLGTLTISFPIPWAGTLRDPNGNPVQQARLRVYALLGR
                     EADGPADHPVLPGVLPIAETRTDKHGAFTLLLPSEVEGI"
BASE COUNT          636 a          801 c          936 g          694 t
ORIGIN      
        1 atgcattgcg gcgactgtgg acaaccatgc cctaaggatc gcgactgcct ccagggtgaa
       61 tgtgccaagg gtttccgctc gatgagtgag caagacgccc catcgccccg gcgttccgct
      121 tgcgccgtat ggacaggaac agaactattc gtctgggggg gtaccgcttt taacaacctg
      181 ccgctgggcg atggtggcgc gtacgatccc atcacggatt catggcgtcc gatcaacatc
      241 aatggagcac cgtcggcgcg cggagagccg aattgcgtgg ccatgggaac agacgttttt
      301 gtctggggtg gttcgacaaa tagtgggctg gtcgatggcg gagcgatttg gagttcgacg
      361 caaaacacct ggaaagcgat tgattctcac aaggctccga agtctcgtcg atggccggca
      421 gtggtttgga caggtgaacg agtcctggtg tggggtgggc agacagacga tgggaaatgt
      481 gaacaaagcg gtagtcaata cgatcctgtc ctcaacacgt ggaattcgat gagcgactcg
      541 tcttcgccga aacattgtaa gcgacaggct tatgcgtgga tggatacgtt gctgtatgtc
      601 tacgggggac gaaacgacaa cgatatcgcg ggcaacaaac tgcattcgta cgatcccgct
      661 acggatgtct ggaaagaagt gaacacgatt actctcgctg ctcgctcgaa ttccttcatc
      721 acgccgagcg actcacgctt gctcgtatgg agcgggttca caacgaaaga gtatggggtc
      781 ccggacggtt tcgtcttcga tccgtcgacg tgtacatgga aaccactgac ttctcctcaa
      841 ccgtcatcga tcagaggtcg ggttccgttt cggtcgggat ggtgcgtgtg gacgggtgat
      901 gccatgctgc tggttggcgg tgtcaaagaa ggaaaaataa ttacaaattc aattacttac
      961 aacccaaata ccaagccaca ggatcagtgg tcgaaggcga ttccttggga tcctcccctc
     1021 gagtatgacc agggaattgg cgtatggacc ggaactgagt ttattctctg gggcggatcc
     1081 gatggatcga aacccattct tggtggtagt cgttggatgc cttgatacgt tttcgtccga
     1141 tcgttgcatt ggttctctgt cttggtgtca cgccgtgggt gcatgcccag gggacaggtc
     1201 cgagtgctgt ggacgtagcg acggcaagag aattgtttcg agaagggggg cgtcttgcca
     1261 atgaacagaa gtggcaggag gctcttgatg cgtacgagcg gtcgatggcg cttcggcctt
     1321 cgaacttgac ccgatacagc attggggtga cgttggagcg cttgaatcgc ctcgtggagg
     1381 cgatggaata cttacgggag ttcgttcatt cggaccatga cagcgccacg cgatcctatg
     1441 tggaagcagc aacgcgtctg ctgcgcgaac tggagcgtcg cattggtcga atcaaggtgg
     1501 tgatacctgg caatccgcgg ggagcggagg ttcggttcga caatgtaccg attccttcgg
     1561 tagccattgg tgtgcatcgt cccacggatc ccggtcatcg aaaaatcgaa gtccgtgttc
     1621 ctggctacgt tcctttcgtc aaagaggtgg acttgaaaga gggcgagtcg gtcgagatcc
     1681 cgttcggctg cataccttgg ccgacgggca aggtgccgaa gacgcggagc agtccgagga
     1741 gccagccgcg gtggtatcgg tgggccaaaa tcgaagtacg acagcgatgg tgctcacgat
     1801 cggtggcgcg ggtgtttttg ccacgggtct gggcatgggg ctttggggat acgtcaaagc
     1861 gaaagatgct gactcgggcg aaggtgaaga tgccgatgcg gcccgtcgtc tcgcactcat
     1921 tggggatatt ggtatggggg tggggttggt ggcggcggga attggaacct acctgctttt
     1981 ttcggaggag gattccacgg atagctcgaa agtgcgtgtg gggccatggg cggccgcagg
     2041 agcgggtggg ctcgtagccg tgggccgttt ttagcgcaag ggatgggaaa ggggcgtggt
     2101 cgggccgcag ggcaagcgtg acgtggatcg tcatgaattt cttttccccc cccctcggcg
     2161 ggcttgtgtg taactatgtg aatttatgtg tttttgtaga tataaccccg tgccatcggc
     2221 tttttccccg agcctttcct tcaccttacg acagatgaaa gggtagtccc gcctccgctt
     2281 cgccatccgc aagtctgttt cgatggtttg ccatcggtcg gataagcacg gtattgtcga
     2341 ctcgtcaccg tgcccctctt ctctgcctat tcatgtgcaa ctatttgaaa ttacatattt
     2401 taatcttgtg aacttccgcc gatttcaccc cttgcgtcga cttgcctcga accaagagcc
     2461 tcccctcata tcccctcaac ctccgacgga aggagaagcg tgaaagctcc gtgcttgtcg
     2521 gttcgtgtct ccgctatcgg caaaacgcca ggtagtacgg ggtggtccgc cggaccgtcg
     2581 gcctcccgac cgagcagggc atacacccga agacgcgctt gctgcaccgg attgccgttc
     2641 ggatcccgta atgtacccgc ccacgggatc ggaaaactga tcgttagcgt tcccaacggc
     2701 tcgctcggta ctttcgcgcg tcctaccacc agccaaggaa atctagaaat atccgaaggc
     2761 tgagccgtga tatccaccac cccgggatcg actcccaaga caaacccccc gccagcaccg
     2821 gtcagagtcg atgccgcacg agcgatgagc ggtgccgtgc cgaatattcg ctcggcataa
     2881 gaccccgtgc tcatcgtcga agggcgcgca atgaccgaaa tattttcaat gggacggttt
     2941 gcgatggggt ccactatcgt tccctccacc gcatacatcg tggctacttc aatcgctctt
     3001 cccgattgta ccgcgggaga acgtccaatc gtccacgaag cttgcgaaat cgatagcccc
     3061 gttcgat
//
LOCUS       DAOSOR010000663         3059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_32028_length_3059_cov_4.620839, whole genome shotgun sequence.
ACCESSION   DAOSOR010000663 DAOSOR010000000
VERSION     DAOSOR010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32028_length_3059_cov_4.620839"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="PK710_23280"
     CDS             complement(<1..1206)
                     /locus_tag="PK710_23280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12722.1"
                     /translation="MASLAESVASWLGRVELLARGVDEQRRAALRSLERGDPLAAREH
                     ALALLREVPNSPAGLAILVDACEALWLDDEAVEALQKLCESAPWRGELWVRLAEGLLR
                     LKAPASEVTKALENALEPDMDPSSRRRALLLLADMDLEREDAWRATRWLDALRFHDSD
                     PEIGLRRLELALMLGDRNGIAESLRSVGQPGTLDGRGSLAVGRGRWQLGEPMALDLLI
                     RALLLEARGAEEALAALLAGSHDVVEVKRIREVLQALGIIEEPRFSFSLAMAEGREQD
                     ARRELVRIARAGDIGAARTLVDVALERKDVEALAAAVDVLGARAPSVAAAIVGAYQAW
                     REGRGQEALTTLDSVTEEETSAGKMAHWLRVQVFGMWLRDGEDGSFQRLIDELRKAAS
                     FMDRLDLVGA"
     gene            complement(1293..2999)
                     /locus_tag="PK710_23285"
     CDS             complement(1293..2999)
                     /locus_tag="PK710_23285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12723.1"
                     /translation="MNAPNLLGEWLPWVWVVWFAALLALTARIWRREVLPIRPRRHAA
                     IVLVVVSLGLLIASFLVPFRVDASGLMELFAGTEPWKGVVGKSVLELIRPLSPLDPMR
                     IILLTRVFALGSTAVAIAVVTSIWRAHHHIEKETERIDLVLLSTVGCLLLTPSYASGL
                     LGVFNFWFFPFVMLGMLLALVDLERRPSWLGRILFWTSLPLLAYSRPETLVATLSMTF
                     LVILSFRKRGDRLTWVSTAVLAFAMTAIAPSVFAYLDDRVRNQPLLMGGDAAGDSSSW
                     ATGWILFRRIIQHLPINGLAVGVAMHGMGLLAMVGMVRRVRARQASVGQVAAFVFFLV
                     EFVAIGVHREGFLRYDKYGLLVVSAIWFLALSTLPEYRASTRRVLLRIGLIGSALLFV
                     FHVTLGMLGVGRCVLPHEPVRDAKLRDMTLLWEDTPRWVRAKCDHGTTNRVVVVMLDE
                     RGEMPEQLDKRADGAKRCEPERWPLVHQWKRAGCATDLFIPDRVESIDEILCEPSDGK
                     HGNPLYSRRTSDHPTLGIISFHRREHVDWVREFLHRRADCGWQMEEESGAALLLRRAH
                     GR"
BASE COUNT          735 a         1046 c          729 g          549 t
ORIGIN      
        1 agcccccacc agatccaacc gatccatgaa ggacgctgcc tttcgaagtt catcgatcag
       61 ccgctgaaac gacccgtcct caccatctcg taaccacatc ccaaacacct gcacgcgaag
      121 ccaatgcgcc atcttgcccg ccgatgtttc ctcctcggtg accgaatcga gcgtcgtgag
      181 agcctcttgt ccccggccct cccgccatgc ttggtaagcc ccgacaatcg cggccgcaac
      241 actcggagca cgcgcgccca atacgtccac ggccgccgct aacgcctcga cgtcttttcg
      301 ctccaaagct acgtccacca acgttcgcgc cgcgcctatg tctcccgctc gcgcgatgcg
      361 aaccaattct cgacgcgcat cctgttcgcg cccttcggcc atcgccaagg agaacgagaa
      421 acgaggctcc tcgatgattc caagtgcctg taatacttca cgaattcgct tgacctccac
      481 cacgtcatgg gaacctgcca gaagcgctgc cagcgcttcc tcggctcccc gcgcttcgag
      541 aagaagcgca cgaatcaaca aatccaaagc catcggctca ccaagttgcc agcggcctcg
      601 gcccaccgct aacgaccctc gcccgtcgag cgtgccgggc tgccccacgc ttcgaagcga
      661 ctccgctatc ccattacggt cgcctagcat cagcgcaagc tccaaccttc gaagtccaat
      721 ctccggatcg ctgtcatgaa aacgaagcgc atcgagccat cgcgtggccc gccacgcatc
      781 ctctctctcc aaatccatgt cagccagaag aagcaaggcg cgacgacgag aggatggatc
      841 catatccggt tccagggcgt tctcgagggc tttcgttacc tcggatgccg gagccttcag
      901 ccgcaacagc ccttcggcaa gacgaaccca tagttcccct cgccacggcg cggattcaca
      961 cagcttttgc aatgcctcga ccgcctcgtc gtccaaccat agcgcctcac aagcatccac
     1021 caaaatggcc agccccgcgg gtgaattggg tacctcccgc aacaacgcca gcgcatgctc
     1081 tcgtgcagcc agcggatccc ctcgttctag acttcgcaac gcggcgcgac gttgctcgtc
     1141 cacccctcgc gcaagaagct ccacacgtcc taaccagctc gctacactct ccgcaaggct
     1201 tgccacggga tgagactata ccagcgccgc gaacggagcc atgcctcacc ttcaaccctt
     1261 ccgccattcc ttatcccaac ggcgtttcga tctcatcgac catgggcgcg acgcaatagc
     1321 agggcagcac cggattcctc ttccatttgc caaccgcaat ccgcccgccg atgcaaaaat
     1381 tctcgcaccc aatcgacgtg ctctcgcctg tggaaactga tgatccccaa cgttggatga
     1441 tctgatgtcc gtcgtgaata tagtgggttg ccgtgttttc catcagacgg ctcgcatagg
     1501 atttcgtcta tcgattcgac cctgtcggga atgaaaagat ccgtcgcgca tccagcgcgc
     1561 ttccattgat gcaccaaagg ccagcgttcc ggctcacacc tcttcgcccc atccgcccgc
     1621 ttgtccagtt gctcgggcat ctcgccgcgt tcatcgagca tcacgacaac gacgcgattc
     1681 gttgttccat ggtcgcactt ggcgcgaacc caccgaggtg tatcctccca caacaatgtc
     1741 atatcccgaa gcttagcgtc tcggacgggt tcatgcggca acacacatcg accaacgccc
     1801 agcatgccca acgtaacgtg aaacacaaat aatagggcgg aaccaatcaa gcctatacgt
     1861 agtaacactc gccgtgtgct cgctcgatac tctggcaatg tcgacaaagc caaaaaccaa
     1921 atcgcggaca ccaccagcaa tccgtatttg tcgtagcgaa gaaagccttc ccgatgaaca
     1981 ccgatggcta cgaattccac aaggaaaaaa acaaacgccg cgacttggcc cacgctcgct
     2041 tgtcgcgcac gcacccgccg caccatcccg accatcgcca gcaaacccat cccatgcatc
     2101 gccacgccga cagctagccc gtttatcggt aagtgctgga taatacgtcg gaataagatc
     2161 catcccgtgg cccaggacga actgtcgccc gcggcatctc cgcccatcaa caatggctga
     2221 tttcgtacgc gatcatcgag atacgcgaat accgacgggg cgatggcggt catggcaaag
     2281 gcaagcaccg ccgtgcttac ccacgtcagt cgatcaccac gtttgcggaa agaaaggatg
     2341 accagaaacg tcatcgacaa cgtagccacc agcgtttcgg gcctcgagta agcgagcaaa
     2401 ggcaagctcg tccaaaacaa tatgcgacca agccacgaag gccttcgttc aagatcgaca
     2461 agggccaaca gcatcccgag catgacgaaa ggaaagaacc agaagttgaa gacacccaac
     2521 aatcccgagg cgtaacttgg tgtgagaagc aggcagccaa ccgtggatag aagaacgaga
     2581 tcgattcgct cggtttcctt ttcgatgtga tgatgtgctc gccatatcga cgtcaccacg
     2641 gcaatggcca cggcagtcga tccaagcgcg aaaacacgtg tcagcagaat gattcgcatc
     2701 ggatccagag gcgacagcgg acggatcaat tccaagacgc tttttcctac cacccctttc
     2761 cacggttccg ttcctgcgaa aagctccatc aaaccgcttg cgtctacccg aaacggtacc
     2821 agaaacgatg cgatgagtag cccgaggctt acgacaacca acacgattgc agcgtggcgg
     2881 cgtggtcgaa tcggcagcac ctcgcgccgc catatacgag ccgtaagcgc aaggagggct
     2941 gcaaaccaca ccacccatac ccagggtagc cattcaccga gaagattcgg cgcattcatg
     3001 aacgccatcg atcatgcacg agtcggcgca tcgattcgag gcaagaatga gaatccctc
//
LOCUS       DAOSOR010000664         3052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_32131_length_3052_cov_4.738405, whole genome shotgun sequence.
ACCESSION   DAOSOR010000664 DAOSOR010000000
VERSION     DAOSOR010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32131_length_3052_cov_4.738405"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="PK710_23290"
     CDS             complement(<1..1191)
                     /locus_tag="PK710_23290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT12724.1"
                     /translation="MPHPPWKSREGLACVIEDWLASRIIQPCFAAERSFAPSQAQTVD
                     LPEDLAPCLRQALAKRSIVQLYTHQAGAFALARGLPDPEAQNPAGPRCIVVATPTASG
                     KSLCFHLPVLQAIVENPSARAMYVFPTKALSRDQESSLHGLISDAQLSIPAIVYDGDT
                     PGDARRVARERSPIILTNPDMLHTGILPHHANWAKTFQALRYVIIDELHTYRGVFGSH
                     MAHVIARLRRIAQFHGSDPVFLCATATIGNPRAHAARLTNVAESDVALLDRSGAAKGP
                     RRFFFYNPPVVNAELGIRASYLKSAVRLTADLLRANVSTLVFGQSRNSVELMLKYLRD
                     RLAGEVPKDAIMAYRGGYLPADRRAIEQGLREGVIRCVVATNALELGIDVGELQAVVC
                     AGYPG"
     gene            1353..2177
                     /gene="lspA"
                     /locus_tag="PK710_23295"
     CDS             1353..2177
                     /gene="lspA"
                     /locus_tag="PK710_23295"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOT12725.1"
                     /translation="MNRRPEHEPPVESESLTPEVREVPNPPVSQDTPTPSVSVTQDRP
                     KPSYVLLGIVSTVALALDLGTKWWALHTLEKPLAHGEGIPAPIQINSWLTFVLARNRG
                     GAWGLLQTANEEIRKPFFLVISLVAIGFIVYLFRRVHPKQHALKWGLPLVLGGAIGNL
                     VDRIRYGWVIDFIDYRASWVRWLNERIASLSPTHGITDHWPTFNIADVAICVGVGLMA
                     IDMFTSRAHHHRRDTTKVVSLQPEQGSAVEPNSPAASSNPPPSDPTSSGLAGQSKV"
     gene            2182..>3052
                     /locus_tag="PK710_23300"
     CDS             2182..>3052
                     /locus_tag="PK710_23300"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOT12726.1"
                     /translation="MQSQLFRLFDLGAPSYFVLLVTGFLFAATLGILWAKRIGRNPDV
                     IVDLALGMLLAGVAGARIMHVLVDGYFWDYVHLCTNPSLVDWQITKDACLSDRYDGVW
                     DAAKGVCHPQKSDCFAWAAFWAGGLTYYGGFIGASVVAWFLLKRDRFPFWKAADMAGF
                     AIATGLAFGRMGCLLAGCCFGVHNDGPLALIFPPGSPASESQFKLGLLPSSRVASLPV
                     LPAQLGEAIASLAIAAFCLLVVHPRKRYDGHVFVAFVMLYALARFLLEFFRADDRGGL
                     WALSTSQWVGIILI"
BASE COUNT          597 a          884 c          865 g          706 t
ORIGIN      
        1 gccgggatat cccgcgcaga ccaccgcttg aagctcccca acatcgatac ctagctccaa
       61 cgcatttgtc gccaccacgc atcggatgac tccctcgcga agcccttgct cgatggcgcg
      121 acgatccgcg gggaggtagc ctcctcgata cgccatgatc gcgtctttcg gcacttcccc
      181 ggcaagtcga tctcgcaaat acttgagcat gagttcgacc gaattgcggg attggccgaa
      241 aacgagggtc gacacgtttg cccgcagcag gtcggcagtc aatcgaaccg ctgatttgag
      301 gtacgaagcg cgaatgccaa gctccgcgtt gacgaccgga gggttgtaaa aaaagaaacg
      361 ccgaggtcct ttcgcggctc ccgagcggtc gagaagagcc acgtctgatt ccgccacgtt
      421 cgtgagcctg gcggcatgcg cgcgcggatt gcctatggtg gcggtcgcgc ataagaaaac
      481 tggatcggaa ccgtggaatt gcgcgatacg acgcaatctc gcgatgacgt gagccatgtg
      541 ggaaccaaaa acgcctcgat aggtatgaag ttcatcgatg atgacgtagc gaagggcctg
      601 aaaggttttc gcccaattcg cgtggtgcgg aaggatgccc gtatgcagca tatcgggatt
      661 ggtcaaaatg atgggggaac gttctcgcgc tactcgacga gcatccccag gcgtatcgcc
      721 gtcataaact atcgcgggga tcgatagttg cgcatcggag atgagcccgt gcaaactgct
      781 ttcttgatct cgcgacagcg ctttggtggg aaagacgtac atcgcgcggg cggacggatt
      841 ctctacgatg gcctgcaaga ctggcagatg aaagcacagg ctttttcccg aggcggtggg
      901 cgttgccacc acgatgcatc ggggaccggc agggttctgg gcttcggggt ccggcaagcc
      961 gcgggcgagg gcaaatgccc ctgcctggtg ggtatatagc tggacaatcg aacgtttggc
     1021 gagggcctga cgcaagcaag gtgcgaggtc ttccggcaaa tcgaccgttt gcgcctggct
     1081 gggagcaaac gagcgttccg ctgcaaaaca gggttgaatg atgcgcgatg ccagccagtc
     1141 ttcgatgacg caagcaagcc cttcgcgtga tttccaggga ggatgtggca tggtggttct
     1201 cagcgtcgcg gccagggatc tcagagatat ctcaaccgat taacctagac aagcgttccg
     1261 tattgatgtc ctctgcgatg ctaccgcttt gcaatcctgg cgttgccacg cagattatcg
     1321 taaaccatgc cttccgcatc gcgaccctat ccatgaaccg acgtcctgaa cacgagccgc
     1381 ccgtggagag cgaatccctc acccctgaag tccgggaggt acctaaccct cccgtctccc
     1441 aagacacacc cacgccttcg gtatccgtga cgcaagaccg acccaagcca tcctatgtat
     1501 tgcttgggat cgtgtcgacg gtggcccttg cgctggattt gggaaccaag tggtgggcgt
     1561 tgcataccct cgagaaaccc ctagctcatg gggaaggcat cccagctccc atccagatca
     1621 actcttggct taccttcgtg ttggcccgta atcgaggcgg tgcctggggc ttgttgcaga
     1681 ccgccaacga ggagattcgt aagccttttt tcctcgtcat ctccttggtg gccatcggct
     1741 tcatcgtcta tcttttccga agagttcatc ccaaacaaca tgctctcaag tggggattgc
     1801 ctctcgtttt gggaggtgcc atcggaaatc tcgtagaccg cattcgctat ggttgggtca
     1861 tcgatttcat cgactacaga gcatcctggg ttcgctggct caacgaacgc attgcttccc
     1921 tatcgccaac ccatggcatc acggaccatt ggccaacctt caacatcgct gatgtggcga
     1981 tctgcgtggg tgttggtttg atggccatcg atatgttcac cagccgcgcg caccatcacc
     2041 ggcgcgacac gacaaaagtc gtatctctcc aaccggaaca gggttctgcg gtggagccga
     2101 attcgccagc ggcatcgtcg aatccgccgc cctccgatcc cacttcttcc gggctcgctg
     2161 ggcaatcgaa ggtttgaggg catgcaatcg cagcttttcc ggctgttcga tcttggcgca
     2221 ccaagctact tcgtgctgct cgtcaccggg ttccttttcg ccgcaacact aggaattctt
     2281 tgggcaaaac gcattggtcg caatccagac gtcatcgtcg atctcgccct cggaatgctg
     2341 ctagctggcg ttgcgggcgc gcgcatcatg cacgtgctcg tcgatggcta tttctgggac
     2401 tatgttcatc tttgcaccaa tccctctttg gtagattggc aaatcaccaa agacgcatgt
     2461 ttgtccgatc gttatgatgg cgtctgggat gcggccaaag gggtatgcca tcctcagaaa
     2521 tccgattgtt tcgcgtgggc cgcgttttgg gccggtggtt tgacgtatta tggaggtttc
     2581 atcggcgcat ccgtcgttgc gtggttcctc ttgaaacgcg accgttttcc attttggaaa
     2641 gccgcggata tggcggggtt tgccatcgcc accgggctag ctttcggccg tatgggatgt
     2701 ttgctcgcgg gatgctgttt cggcgtacac aatgatggtc ccctcgccct catctttcct
     2761 ccaggaagcc ctgcaagcga atcgcagttc aaactcggtt tgctcccgag ttcccgcgtc
     2821 gcctccctgc ctgttcttcc cgctcaactc ggcgaagcca tcgcttcact cgccatcgcc
     2881 gctttctgtt tgctcgtcgt tcacccacga aaacgctatg acggccatgt attcgtggca
     2941 ttcgtgatgt tgtatgccct ggccaggttc cttctcgagt ttttccgagc cgatgatcgg
     3001 ggcggactat gggcgctgtc cacatcccaa tgggttggaa tcattctcat ag
//
LOCUS       DAOSOR010000665         3022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_32556_length_3022_cov_4.876980, whole genome shotgun sequence.
ACCESSION   DAOSOR010000665 DAOSOR010000000
VERSION     DAOSOR010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32556_length_3022_cov_4.876980"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PK710_23305"
     CDS             complement(<1..334)
                     /locus_tag="PK710_23305"
                     /inference="COORDINATES: protein motif:HMM:NF009726.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HOT12727.1"
                     /translation="MTRQGQAGRQGADVRSDCHVACTLRDEGGIDLEIESKVATMFGH
                     KIRKQVLDILADMNVAHATVQVQDGGALPFTLDARVEAALRAADPSLKRNRWIEAKAG
                     WDTPTRRDR"
     gene            complement(376..2181)
                     /locus_tag="PK710_23310"
     CDS             complement(376..2181)
                     /locus_tag="PK710_23310"
                     /inference="COORDINATES: protein motif:HMM:NF012550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOT12728.1"
                     /translation="MGRTIVEKILGTKSNCDARAGDIVDIHIDARVARDFGGANVVKH
                     IQSTGLRVHDPARTYFTFDCNPTGSDQKYATNQQTCRMFARDHGIRVFDIDQGIGTHL
                     AMDRGLIGPGDTFVSTDSHANILGAICAFGQGMGDQDIAHAFAHGTVWFRVPPSVRIE
                     LRGTPAPDATPKDITLALLRRFGANGLLGVAAEMYGTYIDNLDLPGRITMASMFTEMG
                     AIIGLFPVTDTIAQQLSTISKRPFTIMHADPDAEYVERYVVDIEGIGPMISRPGHPED
                     VVPVTEVEGIPIDSALVGSCTNGRIDDIRAAARVLSGRHVAESVVFKIVPSTDAVWNL
                     ALQDGLLRELKQAGVLVGNAGCAGCAAGQVGQNGPGEVTVTTGNRNFAGKQGKGDVYL
                     ASPETVAASAVAGCITTAKRLAAGAYTRKARKTHLEAAQKEAATRRQVPTEVTGRVWR
                     IPVDNIDTDMIFHNRHLAITARQEMGQYAFGNLQGFEHFAKQAKPGDIIVTGKNFGAG
                     SSRQQAVDCFLALGISVIVAESFGAIYERNAINVGMPILRAPALAEVQDGDTVHVDFR
                     TGVVSIPSRGISVKGQPFSDVQLRIYERGGLLVAG"
     gene            2411..>3022
                     /locus_tag="PK710_23315"
     CDS             2411..>3022
                     /locus_tag="PK710_23315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12729.1"
                     /translation="MREEKAIVEGLARTHLPRTLDFSYFLNLFVQLRWIAAACAGLVV
                     FLAQFALRLDSFAYPWLYSIVASILVYNSLFFVVLRLPPAKAQGGESQDDLLYRRGRL
                     GALIQILFDLIALFGLLHFSGGLTNPFVLFFLFHVVVAGILLEPSRAIAVACLTCALI
                     FCLGLFERMGWLSHYRSVEILGSTDPLQSWLFALGLPGVMTITF"
BASE COUNT          581 a          849 c          896 g          696 t
ORIGIN      
        1 accgatcacg tcgtgtcggc gtgtcccaac cggcttttgc ctcgatccat cggtttcgct
       61 tcaacgaagg gtcggcggct cggagggccg cctccacccg cgcatccaac gtgaagggca
      121 aagccccccc gtcctgcacc tgcaccgtgg catgagccac attcatgtcc gccaaaatat
      181 caagcacctg cttgcggatc ttgtggccga acatcgtggc gactttcgat tcgatctcga
      241 gatcgatgcc cccttcgtcg cgcagtgtac aggcgacatg acaatcggat cgaacgtccg
      301 cgccttgcct tccggcctga ccttgtctcg tcatgtttgt tctcctgctg tgaatcagag
      361 cccatggatc ggggttcaac ccgcgaccag aaggcctcct cgctcataaa tacgaagttg
      421 aacatcggaa aacggctgtc cctttacgga tattccgcgg gaagggatgc tcacgacacc
      481 cgttcggaaa tccacatgga ccgtgtcgcc gtcttgcact tcggcaaggg caggggcgcg
      541 taaaatcggc atgcccacgt tgatcgcgtt gcgttcgtag atggcgccga aagactcggc
      601 gacaatgacg ctgattccca gcgccagaaa gcaatccacg gcttgttgac gagaactacc
      661 cgcaccgaag ttttttcccg tcacgatgat gtcgccgggt ttggcttgct tcgcaaagtg
      721 ttcaaacccc tgcaagttgc caaaggcgta ttgtcccatc tcctgccggg cggtgatggc
      781 gagatgccta ttatgaaaaa tcatatcggt atcgatgtta tcgacaggga ttcgccacac
      841 ccttcccgtg acctcggtgg ggacttgccg tcgagtcgca gcctcttttt gagccgcttc
      901 gagatgtgtt tttctagctt ttcgtgtgta agcgcccgcg gccaggcgct ttgcagtcgt
      961 gatgcatccg gccacggcag aggccgccac cgtttccgga gaggcaagat acacgtcccc
     1021 tttgccttgt ttaccagcaa aatttcgatt tcccgtcgtg accgtcacct ccccaggacc
     1081 gttttgaccc acttgacccg cggcgcaacc cgcgcatccc gcgttgccca cgagcacgcc
     1141 cgcctgcttg agttcgcgca gcagtccgtc ttgcaaggca agattccaca ccgcatcggt
     1201 ggaaggcacg atcttgaata cgacactttc ggctacatgc cttccggaaa gcacgcgcgc
     1261 cgccgcgcga atatcgtcga tgcgaccatt ggtgcaagag ccaacgagcg ccgaatcgat
     1321 cggaatgcct tcgacttcgg tgacgggaac cacgtcttcg ggatgacctg gacgcgagat
     1381 catcgggccg atgccttcga tatcgacgac ataccgttcg acatactcgg cgtccgggtc
     1441 ggcatgcatg atggtgaaag gacgtttcga gatggtggaa agctgttggg cgatggtgtc
     1501 cgtgacaggg aaaagtccaa tgattgcacc catttccgta aacatggacg ccatggtgat
     1561 ccgtcccgga agatcgaggt tgtcgatata ggtgccgtac atctcggcgg cgacaccgag
     1621 cagcccattg gcgccgaaac gtcggagcaa ggcgagcgtg atatccttgg gggtggcatc
     1681 gggcgcgggc gtgccacgca actcgatgcg aacggaaggc ggaacacgga accagacggt
     1741 gccgtgagcg aaggcgtgtg cgatatcttg atcgcccatg ccctgtccaa aggcacagat
     1801 ggcgccgagg atgttggcgt gcgaatcggt ggaaacgaag gtgtctccag gaccgatcag
     1861 gccacggtcc atggcgagat gcgtaccgat gccttggtcg atgtcgaaga cgcggatgcc
     1921 atgatcgcgc gcgaacatgc ggcaggtttg ttggttggtg gcgtatttct gatcgctgcc
     1981 ggtgggattg caatcgaagg tgaagtaagt gcgcgcgggg tcgtggacac gcaatcctgt
     2041 ggattggatg tgtttgacga cgttggcgcc gccgaagtcg cgtgccacgc gagcgtcgat
     2101 gtggatatcg acgatatcgc cggcgcgagc atcgcaattg cttttggtac cgaggatttt
     2161 ttcgacgatg gtcctgccca tgggacagcc tttctatcgt tttcgatggg tatcgcgagt
     2221 tgcggcggac cttgccttcg ggaccaaaac gggtcaaggc cgagtcgcag gggcgcgtga
     2281 tggaagtggc ggtggatagc cagggctatt cgagccaatg tcacgggtgg ccgcgaagag
     2341 acggaaccag tcggaaccgg acatggcagg cttgaacggc gagtccggtc aggggtaaaa
     2401 taacgtcgac atgcgggagg agaaggcaat cgtcgagggt cttgcgcgga cacatctacc
     2461 gcgaaccctc gatttttcct atttcctcaa cctatttgtg caacttcgct ggattgcggc
     2521 agcctgcgcg ggcttggtcg tttttctggc ccaattcgct ctgcgcttgg atagctttgc
     2581 ctatccgtgg ctttactcga tcgtagcctc gattcttgtc tataatagct tgtttttcgt
     2641 ggtgctgcgc ctgccccctg cgaaagcgca gggcggagaa tcgcaggacg atttgcttta
     2701 tcgcagagga cggctcgggg cgctcatcca aatcctattc gatttgattg cgcttttcgg
     2761 cttgttgcac ttctccggag ggctgacgaa tcccttcgtt cttttctttc tatttcatgt
     2821 ggtcgtagcc ggcattttgc tcgaaccctc acgggccatt gcggtggctt gcttaacgtg
     2881 cgccctgatt ttttgccttg gtttgttcga aagaatggga tggctctcgc attatcgatc
     2941 cgtggaaatc ctcggttcga ccgacccatt gcagagttgg cttttcgcgc tcggacttcc
     3001 aggcgtcatg acgatcacct tt
//
LOCUS       DAOSOR010000666         3022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_32558_length_3022_cov_4.147961, whole genome shotgun sequence.
ACCESSION   DAOSOR010000666 DAOSOR010000000
VERSION     DAOSOR010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32558_length_3022_cov_4.147961"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PK710_23320"
     CDS             complement(<1..446)
                     /locus_tag="PK710_23320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12730.1"
                     /translation="MMRHAYGWLLASGVWFNVLSCGGVTSHDGAMSSTPVSAQESTPS
                     WNDASTTDGAASAAGGGFCVGSSKVQHGGVMVDPASVTSSPLVMDCCSGFMFRLHTKS
                     QLGTDLSVIVKSFEMFRSGDYAFGPEKGPLEVTVSGGDVPLWSGTAG"
     gene            complement(604..2406)
                     /locus_tag="PK710_23325"
     CDS             complement(604..2406)
                     /locus_tag="PK710_23325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12731.1"
                     /translation="MFGASRFRQFFDFLSLWLAVAIPGLAALQRVAWDSVWRDDLPLV
                     RGLGLVAPGGPSTLTTVAMQLASLFPLGPLPFRAALISALALSICSTFVFLMARGVLE
                     THVPGSFLNAPLAGVAALSASLGPGLQAEATVGGGATVPLAAGLAALWAITHPQWSKE
                     RRHGVCAILSGALLTESALAALVVLLACAVSMVLRKDWPGNRREWIAWGLWPLTVGVL
                     MMPAWFRPLSPNSILNYGRDFSMAELAMVDPVARPATGLAAWKAEVGVVSLVIAVLGG
                     VMGLVRKRTRWLVAPWLLVVAADAVFPASHGGVLTVDQLTALRSFAIVAIAMLASVGV
                     QTVVTTLTDTALPMAKAAAVLLVLFDGTLVAVTAENAAFSVDRSVQPGASAYTDQALY
                     RIDPNAMVLVRSHAIIWRLLASQVVAGARPDVVVVPFPLMGRGTVAAGVLAHEPAATM
                     MLRDMALEGTAGPHAVSRVSDRRRLLVELDPAWKDEMAVHLKAEGLWLSMETQPLGRS
                     DRRLAAENAGKVIERVAKAASSQPADEATQSVLLTVAKQQTLAAALAKDRQVTYDLLQ
                     KMAAVAPQDLFVRALKQRMEHAKGATIDVSGLIR"
     gene            complement(2490..2879)
                     /locus_tag="PK710_23330"
     CDS             complement(2490..2879)
                     /locus_tag="PK710_23330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HOT12732.1"
                     /translation="MSEPDNRRQTERTPIELQVEYRRKNMFFADYTRNISKGGTFIRT
                     EKPMPIGTEFVFSLVVPGFEKPLTLVGKVVWSVSDSDATEANPAGIGIEFQYRDETER
                     QRIDALVRKLMVQELGSKLVKRLLDND"
BASE COUNT          690 a         1031 c          762 g          539 t
ORIGIN      
        1 ccggccgttc ccgaccataa ggggacgtcg cccccgctga ccgtgacttc taacgggcct
       61 ttctcaggcc caaacgcgta atcacccgac cgaaacatct cgaacgactt cacgatcacc
      121 gacaaatccg taccaagctg tgacttggta tgaagccgaa acatgaaacc gctacaacaa
      181 tccatcacca gcggtgatga cgttaccgat gccggatcca ccatcacacc accatgctgc
      241 accttgctgg aacccacaca aaacccaccc ccagccgctg atgctgcccc gtccgtcgtc
      301 gaggcatcat tccacgacgg tgtgctttcc tgagccgaga caggggtgga cgacatggcg
      361 ccgtcatggg aggttacgcc cccacaggac aacacattga accatacccc tgatgcgagt
      421 aaccaaccat acgcgtgccg catcatctat acctcccgat tcgaccagca tctggtagac
      481 aaatcaagca atgccatggc cccaccgtct cggcaaggcg gaatgctttg atgaatcacc
      541 gtcgtcttgc cgaccccagg gccctttgcg gtttcgccca gagctgtttc gcaatcgtcg
      601 cattcagcgg atcagcccag aaacatcgat ggtagcccct ttggcatgct ccatccgttg
      661 cttcaacgcg cggacgaaaa ggtcctgggg ggcgacagcc gccatttttt gcagcaggtc
      721 ataggttacc tgacggtctt tggccagggc tgcggcgagg gtttgctgtt ttgccacagt
      781 gagaagcacc gattgggtgg cttcatccgc gggttggctc gatgccgctt tggccacccg
      841 ctcgatcact tttcctgcat tttccgccgc caatctgcgg tccgaacggc caaggggctg
      901 cgtttccatc gacagccaca acccctctgc cttcaaatgg accgccattt cgtctttcca
      961 cgccggatcc aactccacca acaagcgtcg acggtccgac acgcgcgata ccgcgtgcgg
     1021 ccccgcggtg ccttcgagcg ccatgtctcg aagcatcatg gttgcggcag gctcatgcgc
     1081 gagaacccca gccgccaccg ttccccgacc catgagggga aaaggaacca cgacgacatc
     1141 ggggcgtgct cccgcaacca cctgcgatgc caaaagccgc cagatgatcg cgtgggaacg
     1201 cacgagcacc atggcgttgg ggtcaatgcg gtacaaggct tggtccgtat acgccgatgc
     1261 gcctggttga accgaacgat ccacggaaaa cgccgcattt tccgcggtga ctgccaccag
     1321 cgttccatcg aatagcacga ggagcaccgc ggcggccttg gccatgggaa gcgccgtatc
     1381 cgtcaacgtc gtcaccaccg tttgcacgcc cacgctggca agcatcgcaa tcgcaacgat
     1441 cgcgaaactc cgcaacgcgg tgagttggtc gaccgtgagc acgccaccgt gactggcggg
     1501 aaatacggca tccgccgcaa ccacgagcag ccagggcgcc accagccatc gagttcgctt
     1561 tcgtacgagc cccatgaccc cacccaacac ggcaatcacc agcgaaacca cgccgacttc
     1621 cgctttccaa gcagccagcc cggtggcagg acgagccacc ggatcgacca tcgccagttc
     1681 cgccatcgaa aaatctcgcc cataattgag aatggaattc ggcgataagg ggcgaaacca
     1741 cgccggcatc atcagaacac ccacggtcag aggccatagc ccccaggcaa tccattctcg
     1801 acgatttccg ggccaatcct ttcgcaatac catcgacact gcgcaggcca gcaggaccac
     1861 caaggccgcc aaggcacttt cggtgagcag cgcgcccgac aggatggcac acacaccatg
     1921 gcgtcgctct ttcgaccatt gcggatgggt gatagcccaa agcgcggcca atccagcggc
     1981 aagaggtacg gtcgcacctc cccccaccgt tgcttctgct tgcaatcctg ggcccaacga
     2041 cgccgaaaga gcggccaccc cagccagggg cgcattcaaa aaagagcctg gaacatgggt
     2101 ttccaacacg ccccgggcca tcaaaaacac gaacgtcgag cagatagaca aggccaatgc
     2161 ggagatcaac gcggcacgga aaggtagcgg accaagagga aagagcgagg ccagttgcat
     2221 cgccacggtc gtgagcgtcg atggcccccc cggcgccacc agcccaagtc cgcgcaccaa
     2281 cggcaaatca tcacgccaga ccgaatccca ggccacacgt tgcaacgcgg ccaatcccgg
     2341 aatggctacc gccagccata aggataggaa atcgaaaaat tgtcggaaac gggatgcgcc
     2401 aaacaccgtc tctcccgtgc cacgatcttg aaaaaaaggc caaatagctg cctactcaac
     2461 cccaatggcg cggtccctca cccacgagat caatcattgt ccagcaatcg cttcaccaat
     2521 ttcgaaccta gctcttgcac catgagcttg cgaacgagcg cgtcgattcg ttgccgctcc
     2581 gtttcatcac ggtattgaaa ttcaatccca atgcctgccg gattcgcttc cgtggcgtcg
     2641 gaatcgctca cgctccacac cacttttccc acgagcgtca agggtttctc gaatcccggc
     2701 accaccaacg aaaagacgaa ctccgtaccg ataggcatcg gcttttccgt acggataaac
     2761 gtgccgcctt tggagatgtt tcgcgtatag tccgcgaaaa acatattctt acgccggtat
     2821 tcaacctgca actcgatggg cgtccgctcc gtttggcgtc gattgtcagg ttcgctcatc
     2881 agccagcagc ttagccgatt tgatccccct tgtcatcgcg tctgatgcgc cccacgacgt
     2941 tctccctcca cgctcccaca agcgattcgc cactgtttta ccccgcactt cttcacgcca
     3001 ttccctcgcc agatcatctc tc
//
LOCUS       DAOSOR010000667         2979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_33146_length_2979_cov_4.000342, whole genome shotgun sequence.
ACCESSION   DAOSOR010000667 DAOSOR010000000
VERSION     DAOSOR010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33146_length_2979_cov_4.000342"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PK710_23335"
     CDS             <1..284
                     /locus_tag="PK710_23335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12733.1"
                     /translation="RVQTIIVHVQTIRADLRAGVDEEFSMARSNPCSRLPHGVGLVLA
                     LARKIVESKPNHDESPGMEEFLAFRVFDLMYAVRHEGVSNPPRNPSWRH"
     gene            383..1069
                     /locus_tag="PK710_23340"
     CDS             383..1069
                     /locus_tag="PK710_23340"
                     /inference="COORDINATES: protein motif:HMM:NF022591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HOT12734.1"
                     /translation="MSKPLFWTCVASLSVAMISLVASGWVITNDRKDDEKLGLGRVWD
                     EPEINRVEAGSSVGVGAKTAQLGVSAKSGTVSGGEVEDSKTSEESRANRAELRVLRLV
                     VAHGVRHREPLDEAESFVAGTRPVYAFVELANQSDHPGEIVVDFEKGTTKTGNVGLAV
                     PSNTGRYRTWAYSRGLRQAGSWRVVVRDAHTGRELARKSIEILDSETPNARAEVASDW
                     MVDGDPKGMI"
     gene            1160..2245
                     /locus_tag="PK710_23345"
     CDS             1160..2245
                     /locus_tag="PK710_23345"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HOT12735.1"
                     /translation="MSSSDQRSDPGLDLHRGLAVVLMFLAHALRMQSSRSSSAVFRWI
                     DDTLQWMDRGVAYVAASFLFIVGFSMVLAHRDSASHARAFMGKVSRRAFRLYLMSLLM
                     FFAEHGTDMPDFLLSSGILSVIAVAMVLTAAAITSSRPITALALVVGVVMAVTYGLEA
                     MNVSVSGLNAGPGGAIPLVMFSAMGAWSAKLYLQHGVKAVAAVAMIASPGFVIALTRD
                     EPWLALYESVYKDHGGLAIAHYLGLATPKGEIVSRVFWNPSTIGAIGLVTPVASTTAL
                     MLWKQGRVAHKAALRPLLWLGRHALMVFVAHYALLGMVDLMGLRPSHAGWTLGLWAAL
                     IALCSGMSAVAERYPMSLSFRKKSRAR"
     gene            2291..>2979
                     /locus_tag="PK710_23350"
     CDS             2291..>2979
                     /locus_tag="PK710_23350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOT12736.1"
                     /translation="MNEHVLHSGKALIALVWVLSFCACDRMETKHESSALSPTPSVEP
                     PTRPPIDGGSSRSTLEPDVSSSSVQPVIPPSSTARLEDERNTIDIFRFAAPATVFVTQ
                     KRLVQDWTMRTTEVPAGSGTGFVWDQKGHIVTNYHVVDPSNGRTTYTVTLYNRKSYEA
                     ALVGGEPNKDIAVLRIATPGEELTPIRLPPASQKIEVGQKTIAIGNPFGLDHTLTTGV
                     ISALGREVTGYG"
BASE COUNT          571 a          685 c          974 g          749 t
ORIGIN      
        1 cgcgtgtgca gacgatcatt gtgcatgtgc agacgatccg tgcggatctt cgggcgggag
       61 ttgatgagga attttcgatg gctcggagta atccgtgttc gagattaccg catggggttg
      121 ggttggtttt ggcgctcgcg cgcaagattg tcgaaagcaa accgaaccat gatgagtctc
      181 ctggcatgga agagttcctt gcgttccgtg ttttcgattt gatgtacgcc gttcgccacg
      241 agggtgtgtc aaatccgcca cggaaccctt cttggagaca ctgaaattat attgtaaatc
      301 aataacttgc ggttgttggg gcaattggtc caagcgttgc ttcaaacggt ggcatcactt
      361 catccctggt ggagtcatca tcatgagcaa gccgttgttt tggacctgtg tggcctctct
      421 ttccgtggca atgatttctc ttgtcgcatc gggatgggtc atcacaaacg atcggaagga
      481 tgatgaaaaa ctcggcttag ggcgagtatg ggacgagccg gaaatcaacc gggttgaagc
      541 cgggtcatcg gtgggggttg gagcaaagac ggcgcagctc ggtgtgagcg cgaagagtgg
      601 aacagtgtcg gggggagagg tggaagactc gaaaacgtct gaggaaagcc gagcgaatcg
      661 ggcggagttg cgggtgttgc gtttggtggt cgcgcatggg gttcgtcatc gcgagccttt
      721 ggatgaagcg gagtccttcg tggcagggac tcgaccggta tatgcgtttg tcgagctggc
      781 caatcagtcg gatcatccag gtgagatcgt ggtggatttt gagaaaggga cgacgaaaac
      841 ggggaatgtg ggacttgcgg tgccgtctaa tacgggcagg tatcgtacgt gggcgtattc
      901 ccgtgggttg cggcaagcgg gaagttggcg tgtcgtggtg cgcgatgcgc atacgggtcg
      961 agagcttgcg cgaaaatcca tcgagatatt ggattccgag actcccaacg cgcgagcgga
     1021 ggttgcgtcc gattggatgg tggacggcga tccgaagggt atgatttgaa acggattatt
     1081 tcagggttct ggtgattggt ttctatcttg gctgtcgatt ctcaacgcgt tcgcgattag
     1141 gttctcctgc ttcgatacca tgtcttcgtc cgaccagcgc tccgatcctg ggctcgactt
     1201 acaccgcggt ctggcggtag ttttgatgtt tcttgcgcat gcgttgcgca tgcaaagctc
     1261 tcgttcgtca tcggcagtgt ttcggtggat cgacgatacg ctgcagtgga tggatcgcgg
     1321 tgtagcttat gtggcggcgt cgtttctgtt catcgttggg ttcagcatgg ttttggccca
     1381 tcgggattcg gcctcccatg cacgagcgtt catggggaag gtatcgcgtc gggcctttcg
     1441 gttgtacctt atgtctttgc tcatgttctt cgccgagcac ggcacggata tgccggattt
     1501 tttgctctcc tcgggaattc tgtcggtgat tgcggtggcg atggttctta cggccgcagc
     1561 gatcacatcg agcaggccaa tcactgccct tgcgcttgtg gtaggtgtgg tgatggcggt
     1621 gacctatggg ctcgaggcga tgaacgtttc ggtgtccggg ctgaatgcag gaccgggggg
     1681 ggctataccc ttggtgatgt tttcagcgat gggagcttgg tccgcgaagc tatatctaca
     1741 gcatggagtg aaagccgttg ccgcggtggc gatgattgcc tctcctggat tcgtgattgc
     1801 gctaacgcgt gatgaaccct ggttggcctt gtacgagagc gtgtacaagg atcatggcgg
     1861 ccttgcgatc gcgcattacc tgggtcttgc cacccccaaa ggggagatag tttcgcgtgt
     1921 attttggaat ccgtcgacga tcggcgcgat cggtttggtg actcccgtgg cgtcgacgac
     1981 agcgttgatg ttatggaagc aggggcgcgt agctcacaag gcagcgttac gtccgttgtt
     2041 atggcttggt cgtcatgcgc tgatggtttt cgtggcgcat tatgcgctgc ttgggatggt
     2101 tgatttgatg ggactacggc cttcgcacgc gggttggacc ttgggacttt gggcggcgtt
     2161 gattgcgctt tgttcaggca tgagcgcggt ggcggaacgt tatccaatga gcctgtcgtt
     2221 tcgaaaaaaa tcgcgggcaa gatgaagctc agaccttgct cagggcgggt gtgtttcgta
     2281 tgaaagctcg gtgaacgaac acgttttgca ttctggaaaa gcactgatcg cgctggtctg
     2341 ggttctgtcg ttttgtgctt gcgacagaat ggaaacaaag cacgaatcat cagcgctttc
     2401 gccgacgcct agcgtcgagc caccgacacg tccacccatc gatggcggaa gttcccggtc
     2461 gacgctcgag ccggatgtta gttcttcatc ggtccagccg gtgattcccc caagttccac
     2521 ggctcgtctc gaagacgaac gcaacacgat agacatattt cgttttgcgg cgccggcaac
     2581 ggtatttgtg acacagaagc gtcttgtgca ggattggacg atgcgaacga cggaggtgcc
     2641 ggccgggtcg ggaactggat tcgtatggga tcagaagggg catatcgtga cgaattatca
     2701 cgtggtcgat ccgtcgaatg ggcggaccac gtacacggtg acgctttaca accgcaaatc
     2761 ttatgaagcg gcgttggtgg gaggggaacc caacaaggat atcgcggtgc ttcgaattgc
     2821 cacgcccggg gaagaactca cgccaatccg tttgcctccc gcgtcgcaaa aaatcgaagt
     2881 tgggcagaaa accattgcga ttggcaatcc ctttggtctc gatcatacgt tgacgacggg
     2941 agtcatttcc gcgcttggac gcgaggttac cggttatgg
//
LOCUS       DAOSOR010000668         2976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_33193_length_2976_cov_4.273536, whole genome shotgun sequence.
ACCESSION   DAOSOR010000668 DAOSOR010000000
VERSION     DAOSOR010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33193_length_2976_cov_4.273536"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PK710_23355"
     CDS             complement(<1..332)
                     /locus_tag="PK710_23355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12737.1"
                     /translation="MPNNTDGLAACSISCPHHPRCPGCPLLDLPYPEQCQRKHNTVVD
                     AFRAYPSLRNIEVSDLRAAPTIHSYRIRAKLVVSDDGIGLFAAGTHDIVDTPSCPVLH
                     PLVRQTVQR"
     gene            complement(397..1959)
                     /locus_tag="PK710_23360"
     CDS             complement(397..1959)
                     /locus_tag="PK710_23360"
                     /inference="COORDINATES: protein motif:HMM:NF016138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HOT12738.1"
                     /translation="MSALVKNAAGRLVPTEINGKPAVPYQGVHQFRPSGRKAAPPIRS
                     CADYPADGNKIVGSLREALERCGVKNGMTVGTHHHFRNGDFVANAVMDTLADMGVRDV
                     VWAPSASFPCHAPMVRHLESGVMHHIEGSMNGPLGEYCSAGKMRGLGVLRSHGGRFQA
                     IQDDELHIDLAVIAAPTADPFGNANGVQGPSACGPLGFALADSLYADRVVVVTDNLVP
                     FPCVPWQIHGQNVDYVVVHDRIGDPSKIVSGTTQVTRSPDRLLIAEYIARFVAEVGIM
                     ADGFSFQAGAGGITLAFALYLKELMRKANVKARFIRGGSTKYLVDMLQEGFTDYILDG
                     QTFDLDGVRSMREDPKRHLDTSPFTSYNFHGKGNFASILDCVVLGATEVDRAFNANVV
                     THSDGVLLHGIGGWQNCLTAKCTILAVPSFRDRIPVILDRVTTLCGPGELIDVIATER
                     GIAINPRRQDLIDAAKRCTVPIRSIDDIADEVNRLTGGPPDAPTLGDKVVAAIKWVDG
                     TVIDSVRQSLNC"
     gene            complement(1956..>2976)
                     /locus_tag="PK710_23365"
     CDS             complement(1956..>2976)
                     /locus_tag="PK710_23365"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOT12739.1"
                     /translation="VQDGGALPFTLDARVEAALRAADPSWMRDRWIEAKAGWDTPTRR
                     DRFRRSRLYLPGNEPKFMINAGLYGADGLILDLEDSVAPMHKFAARYLVRNALRILDF
                     GTAERMVRINQGDLGLEDLDVILPHHVHLVLIPKVETAEQVRQVDDRVAMHLGKDHGV
                     LLMPIIESAKGGWFAYDIAMASPSVVALAIGLEDYTADIGTQRTNEGRESLWLRQQVI
                     NAARAAGVQPIDTVFSDVADSEGLRGSVIEAKQLGFDGKGCIHPRQIPIIHEAFAPTR
                     QEIDKAQKIVRAFDVAQQQGLAAVSLGSKMIDPPVVKRAMHTVTLAIACGLLSEDWKT
                     KEVSS"
BASE COUNT          596 a          807 c          962 g          611 t
ORIGIN      
        1 cgctgcacgg tttgtcgtac gagcggatga aggacggggc acgagggggt atcgacgata
       61 tcgtgggtac cggcggcgaa caaaccgatg ccgtcgtcgc tgacgacgag cttggcgcgg
      121 atgcgataag aatggatggt tggcgcggcg cgtagatcgg aaacctcgat atttcgaagg
      181 gaaggatagg cacggaaggc gtcgaccacg gtattatgct tgcgttggca ttgctcgggg
      241 tagggcaggt cgagcaaggg acatcctggg caacgtgggt gatgcgggca ggatatcgag
      301 caggcggcga gcccatcggt gttgtttggc atggcggtgg tctcgaaaga ggacgggaga
      361 gagacgattc acgactatcc gtttacggat agtctatcaa caattcaggg attggcgcac
      421 gctatcgatg acggttccat cgacccattt gatggcggca acgaccttgt ctcccaaggt
      481 gggagcatca ggggggccgc ccgtgagacg attcacttca tcggcgatat catcgatcga
      541 gcggatgggg accgtgcagc gttttgcggc atcgatgaga tcctgtcgcc ggggattgat
      601 ggcaatcccg cgttcggtgg caattacatc gatgagttct ccaggaccgc agagcgtcgt
      661 gacgcgatcg aggatgacgg ggatgcggtc gcggaagctg ggaacggcca ggatcgtaca
      721 ctttgcggtg aggcaatttt gccagccgcc aatgccatgg agcaaaacgc cgtcggagtg
      781 ggtgacgaca ttggcgttga aagctctgtc gacttcggtg gcgccgagca cgacgcagtc
      841 gaggatcgag gcgaaattgc ctttgccgtg gaaattgtag ctggtgaacg ggctggtatc
      901 gaggtgccgt ttggggtcct cgcgcatgga cctcacacca tcgagatcga aggtttgtcc
      961 gtcgaggata tagtcggtga atccttcttg gagcatatcg acgagatatt tggtggagcc
     1021 gccgcgaatg aagcgagctt tgacgtttgc ctttcgcatg agttctttca ggtacaaggc
     1081 gaaggcgagg gtgattccgc cggcgccggc ctgaaaggag aagccgtccg ccatgattcc
     1141 gacttcggcg acgaagcgag cgatatattc ggcgatgagc aatcggtcgg ggcttcgggt
     1201 tacctgggtg gtgccgctga cgattttgct tgggtctccg atgcggtcgt gaacgacgac
     1261 ataatcgacg ttttgtccgt gaatttgcca aggtacacaa gggaagggga cgaggttatc
     1321 ggtgacgacg acgacgcgat ccgcgtacag ggagtcggcg agggcgaagc cgagggggcc
     1381 acaagcgctc ggaccttgaa cgccgttggc gttgccgaag gggtcggcgg tgggagcggc
     1441 gatgaccgcc aggtcgatgt gcaattcatc gtcttggatg gcttggaatc ttccgccgtg
     1501 ggagcgcaat acgccgagac ctcgcatttt tccggcggag caatactcgc caagcgggcc
     1561 attcatgctt ccttcgatat ggtgcatgac cccggattcg aggtggcgaa ccatcggagc
     1621 gtggcatgga aacgaggcgg aaggtgccca aaccacatcg cgtacgccca tgtccgccag
     1681 ggtatccatc acggcattgg ccacgaaatc gccattgcgg aagtgatggt gcgtgcccac
     1741 ggtcattccg tttttcacgc cacagcgttc caaggcttcg cgaaggctgc ccacgatttt
     1801 gttgccgtcg gccgggtagt cggcgcagga acgaatgggc ggagcggcct tgcgtcccga
     1861 gggtcgaaac tggtggacgc cctggtaggg gacggcaggt tttccgttga tttccgtggg
     1921 gacgaggcgt cccgcggcgt ttttaacgag cgcgctcatg aagacacctc cttcgttttc
     1981 cagtcctccg acaacaaccc acaggcaatc gcaagcgtta cggtatgcat ggctcgtttc
     2041 accaccggtg ggtcgatcat tttgcttccc aacgacacgg ccgcgagccc ttgttgctgc
     2101 gccacgtcga aagcacgcac gattttttgc gctttgtcga tctcttggcg cgtgggcgcg
     2161 aaagcctcgt ggatgatcgg aatctgacga ggatgaatgc atcctttgcc atcgaaaccg
     2221 agttgtttgg cttcgatgac gctgccgcga agaccttccg aatccgccac atcggaaaaa
     2281 accgtatcga tgggctgcac ccctgccgca cgggcggcat tgatcacctg ctgacgaagc
     2341 cagagactct cgcgaccctc gttggttcgc tgcgtgccaa tgtccgccgt atagtcctcc
     2401 aagccaatgg cgagcgctac taccgatggc gacgccatcg cgatatcgta ggcaaaccat
     2461 ccgcctttgg cgctctcgat aatcggcatc aacagcacgc cgtggtcttt gccaagatgc
     2521 attgccaccc ggtcatcgac ctgccgaact tgttccgcgg tctcgacctt aggaatcagc
     2581 acgagatgaa cgtgatgcgg caggatgaca tcgaggtcct cgagtcccag gtccccctgg
     2641 ttgatgcgga ccatgcgttc cgcggtgccg aagtccagga tgcgcagcgc gtttcgaacg
     2701 agataacgcg cggcgaactt atgcatgggc gcgacggaat cttcgagatc gaggatgaga
     2761 ccatccgctc cgtagagccc cgcgttgatc atgaatttcg gttcatttcc gggtagatac
     2821 aagcgtgatc gacgaaaccg atcacgtcgt gtcggcgtgt cccaaccggc ttttgcctcg
     2881 atccatcggt ctcgcatcca cgaagggtcc gcggctcgga gcgccgcctc cacccgcgca
     2941 tccaacgtga agggcaaagc ccccccgtcc tgcacc
//
LOCUS       DAOSOR010000669         2965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_33371_length_2965_cov_3.579038, whole genome shotgun sequence.
ACCESSION   DAOSOR010000669 DAOSOR010000000
VERSION     DAOSOR010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33371_length_2965_cov_3.579038"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /gene="efp"
                     /locus_tag="PK710_23370"
     CDS             <1..517
                     /gene="efp"
                     /locus_tag="PK710_23370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOT12740.1"
                     /translation="GKPYTIVEAQFVKPGKGQAFTRTRIKNLQTGAVIDRTFRSGDKL
                     EPADVQVRDMSFIYPDGDGYVFMDESGDQVSVPADVLGESSQFLSDGLAVEIVFFKGE
                     AIDVTMPAHVVLEITHCDPGVRGDTASNVTKPATLSTGAVIQVPLFVNENEWVKVDTR
                     TGAYMERVNKR"
     gene            complement(529..1314)
                     /locus_tag="PK710_23375"
     CDS             complement(529..1314)
                     /locus_tag="PK710_23375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12741.1"
                     /translation="MGSKDILSALGAYERLREQGTGTKELDTIRKRIEKAVPSRSVQA
                     YFLPFGPSVDPSGGTWSPLAFLPDGSLLIRTDNSVMRFDAKLRIALPDPDSGPIAPWS
                     LRVEASGGSTSFEGVEGRDGWLWARWRRGNDTIEALLPFDTSLTKTTARPVAWSSTGL
                     HLLTSLGPVWVSTQGSKVKRTIPEATPGSLGSPRSPDGHQLVHASSLGLVVLDKGNDA
                     TLWRAPSLPNGYEKLAGCVISNGATAVACIDSAMTRVFLNESQ"
     gene            complement(1215..2330)
                     /locus_tag="PK710_23380"
     CDS             complement(1215..2330)
                     /locus_tag="PK710_23380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12742.1"
                     /translation="MRRGVDWQVLFVPTIVSTLMGCDSCEKEKPYVPYTVDPSAMVSG
                     AISSDPAESPTMPSIETFSPVVAKRLNGALSVRTAEGTVSAPAGKAIDLWLEADLTAD
                     GKPDSVAWLRSTDGLTGELVRFVTTRPGEALSLSTLVSLPPDFAMAPDCKATSDLRQV
                     GPRTVALSIRKICPIGTKNVTTQWVAALVLERNPALRFQLIVDQPPIEKLEISLDALD
                     RDGDGFDDLLVSFQSNGSTAAFEEPKTENAVATLRYFDRPAGLSRDPHEPTSSFLAMA
                     QRLERLAKGNAKDSVAPIARATRQLHLSLCGEGDSRIKIMGQPLQCGSRDAMTQSRRR
                     SWMQRWVRKTFFLPSGLTSVFGSKEPARRNWIQSENG"
     gene            complement(2499..>2965)
                     /gene="def"
                     /locus_tag="PK710_23385"
     CDS             complement(2499..>2965)
                     /gene="def"
                     /locus_tag="PK710_23385"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOT12743.1"
                     /translation="ETLADSGNGVGLAAPQIGIGVRAIVVMLDARGNNPHVDCFLNPR
                     IIERSDDLTLDYEGCLSIPDVCGLVKRNRRIVVEHGLFGSRDRLVAEQFDARIFQHEI
                     DHLDGVLYVDRVQGQTHPKDKLKELREQLRAQQPAVACTPRFGGRHRDEILL"
BASE COUNT          650 a          809 c          876 g          630 t
ORIGIN      
        1 tggaaaaccc tataccatcg tggaggccca atttgtgaag cctggcaaag gacaggcttt
       61 tacgcgaacc cgcatcaaga acttgcaaac cggggcagtc attgaccgca ccttccggtc
      121 tggggataaa ctcgaaccag ccgatgtgca agtacgcgac atgtccttca tctatccgga
      181 tggggatggt tacgtattca tggatgagtc gggagatcaa gtgtcggttc ccgcggacgt
      241 gctcggcgaa tcgagtcagt tcttgtccga tgggcttgct gtcgaaatcg tatttttcaa
      301 aggagaggcc atcgacgtta ccatgcctgc gcatgtcgta ctcgagatta ctcattgcga
      361 tccgggcgtt cgcggtgaca cggccagtaa cgtgaccaaa ccggcaacct tgtccaccgg
      421 tgcggtgatt caagttcctc ttttcgtcaa tgagaacgaa tgggtcaaag tcgacacgcg
      481 cacgggtgcc tatatggaac gcgtcaacaa gcgctgatcg gtcatcgttt actgggattc
      541 gttcaagaac acgcgtgtca tggcgctgtc gatgcaagcc actgccgtgg ccccgttcga
      601 tatgacgcag cctgctagct tttcataacc gttcggcagc gatggtgcgc gccagagagt
      661 cgcgtcgttt cctttatcca gcacgacgag acccaggctc gaagcatgaa cgagttggtg
      721 cccatcgggc gagcgcgggc ttcccaagct tccaggggtt gcttccggaa tcgttcgttt
      781 caccttcgaa ccttgtgtgg acacccaaac gggaccaagg ctcgtgagca gatgaaggcc
      841 tgtggaagac caagcaaccg ggcgggcagt cgtctttgtg agggacgtat cgaaaggaag
      901 caatgcctcg atggtatcgt ttcctcggcg ccatcgagcc catagccaac cgtctcgacc
      961 ctcgacccct tcgaatgacg ttgaaccgcc cgaagcttcc acgcgcaggg accaaggtgc
     1021 gatggggccg ctatccggat cgggaagtgc gatacgaagt ttggcgtcga aacgcatcac
     1081 cgaattgtcg gtgcggatga gcagggatcc atccggaaga aaagccagag gactccatgt
     1141 gccacccgac ggatcgaccg aaggtccgaa gggcaggaag taggcttgaa cgcttcttga
     1201 cggaacggcc ttctctatcc gttttcggat tgtatccagt tccttcgtgc cggttccttg
     1261 ctcccgaaga cgctcgtaag ccccgagggc agaaagaatg tctttcgaac ccaacgctgc
     1321 atccagctcc gccgtcgtga ttgagtcatg gcatcgcgag aaccacattg caaaggctgc
     1381 cccatgatct tgatgcggct atcgccttcg ccgcatagcg acaagtggag ttgtcgagtt
     1441 gcacgtgcga tcggcgccac gctgtctttg gcattgcctt tggccaatcg ttcgagtcgt
     1501 tgggccatcg cgagaaacga agacgtgggc tcatggggat ctcgtgacaa gccagcgggt
     1561 cgatcgaagt agcgaagtgt ggctaccgcg ttttcggttt tcggctcctc gaaggctgcg
     1621 gtggacccgt tgctctgaaa cgaaaccaga aggtcgtcga agccatcgcc atcgcggtcg
     1681 agcgcatcca acgaaatctc gagtttttca atcggtggtt ggtcgacgat gagttggaac
     1741 cgcaatgcag ggttacgttc gagcaccagg gctgcgaccc attgtgtggt gacgttcttg
     1801 gttccgatcg ggcagatttt tcgaatcgac agagccacgg ttcgaggacc tacctggcgc
     1861 aaatccgaag ttgccttgca gtccggagcc atcgcaaaat cgggaggaag tgaaacgagt
     1921 gttgaaagcg acaaggcttc gcccggtcgg gtggtaacga agcgtaccag ttctcccgtg
     1981 agaccatctg tgctccgaag ccaggccacg gagtcgggct tgccatcggc cgtcaggtcg
     2041 gcttcgagcc ataaatcgat cgctttaccg gccggagcgg aaaccgtgcc ctccgccgtc
     2101 cgaacagaga gcgcaccgtt gaggcgtttg gccacgactg gtgaaaaggt ttcgatgctc
     2161 ggcatcgttg gcgactcagc gggatccgag gaaatggctc cgctgaccat ggcgctaggg
     2221 tcgaccgtat agggaacgta aggcttttct ttctcacagg agtcgcatcc catgagcgtc
     2281 gagacaatcg tcggaacgaa aagcacttgc caatctacac cgcgtctcat gaagcaaggt
     2341 tgtacggcag aatcgaacga ttcgaaacgg agatcgagtc gagggattgc ggagccaacc
     2401 atccgtgggg cgacaccctt ggatgaggcg tgatcacgca aggcgccaag gtagcgatgg
     2461 atcgctttgg accggactag ggcaaaaatg ttacgggatt acaagaggat ttcatcccgg
     2521 tgtcggccac cgaatcgggg ggtacaggct accgcgggct gctgagcacg caattgttcc
     2581 cgcaattcct tgagtttatc tttgggatgg gtctgacctt gcacacggtc gacataaagc
     2641 acaccatcga gatgatcgat ttcatgctgg aaaattctgg cgtcgaactg ctccgccacg
     2701 agtctatccc ggcttccgaa cagcccatgt tccacgacga tccgacgatt gcgtttgacc
     2761 aatccgcata catcgggaat cgacaggcaa ccttcatagt ccagcgtgag atcatcgctt
     2821 cgctcgatga tgcgggggtt gaggaaacaa tctacgtgcg ggttgttgcc acgggcatcg
     2881 agcatgacca cgatcgcgcg caccccaatg ccaatttgtg gtgcggcgag gcctactcca
     2941 tttcctgaat ctgcaagggt ctcgc
//
LOCUS       DAOSOR010000670         2951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_33566_length_2951_cov_3.527279, whole genome shotgun sequence.
ACCESSION   DAOSOR010000670 DAOSOR010000000
VERSION     DAOSOR010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33566_length_2951_cov_3.527279"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PK710_23390"
     CDS             complement(<1..357)
                     /locus_tag="PK710_23390"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HOT12744.1"
                     /translation="MDRFLVDTLTVLAEPPVMSENDHSSPRRRFLERCAQGSIALGTC
                     VIAWPVYRYLLPPPLDPPDTATSLALGPSRQLLPRQARSATLGSIPIIVLRLEDGSLR
                     AFDATCPHLGCEISFDP"
     gene            490..1998
                     /locus_tag="PK710_23395"
     CDS             490..1998
                     /locus_tag="PK710_23395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT12745.1"
                     /translation="MPGWLSELLPISILLVVVALVLARLPKVKEVEHSAAFRRRRVLN
                     WLPLGLAYAFLYMGRYNLKVSQHVFEQMTNADGQQLMTNAGFATIFGVGTAVYGISFL
                     LNGPLTDRFGGRKAILTGMGGAAAANGLMALTMVIFQRGGPMADSVGRNFLLLFSILY
                     AANMYFQSFGAVAIVKVNAPWFHLRERGVFGAIFGILIALGIYFAFDWSYKIISFFGQ
                     DPHLEQASSRLTWVFAAPATILAALWVVVGIWVKNTPGDAGHEDFDTADASSEEQGKN
                     LGAGQVIKLMLRNPVVMTIASVEFCNGFLRQAIMQWYPTFAKHTNGELGHMDGFVFKN
                     WGLLLCIAGIVGGMVAGVISDHLFQSRRGPVVSILYGGMIVGSIVLFFAYQTSMVGWI
                     AVLMSVLVIGVHGMLSGTASMDFGGKKNVGIVVGIIDGFVYAGTAMMALTYGLLLPDE
                     QLNEAGKIVGPSTQASNWFAWPAAMIPFAIVGFVLALRVWNAKPQSKAASVS"
     gene            complement(2068..>2951)
                     /locus_tag="PK710_23400"
     CDS             complement(2068..>2951)
                     /locus_tag="PK710_23400"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT12746.1"
                     /translation="ACDSGATGAGNNCGNASTQDCCASSDVQGGSFSRSYDGVYYMDD
                     TYVATVADFALDTFEVTVGRFREFIEAGWGTQKNPPSPGAGAHPLIANSGWNAEWNAE
                     LVADKGALTTSLNCDVTYQTWTDTPGPKEKRPINCVTWYEAFAYCAWAGGRLPTEAEW
                     NFAASGGSQHRTYPWGDTLDHDHAVWGCETDGSPGCVAADIVDVGSRSPLGDGLWGHA
                     DLAGSMSEWVLDRFVDPYPMPCSNCAAVQFGSGRMRRGGAWNEMNPNRLRNGSRESAN
                     PSHRLTHMGFRCARSQP"
BASE COUNT          581 a          740 c          973 g          657 t
ORIGIN      
        1 ggggtcgaaa ctgatctcgc agcccagatg cggacaggtg gcgtcgaagg ctcgcaagga
       61 cccatcctcg agccggagca cgatgatggg aatcgagccc aaggtggcgg aacgggcttg
      121 gcgaggcagc agttgccgcg acggccccaa ggcgagggat gtggcagtat ccggtgggtc
      181 caacggggga ggcaagaggt agcgataaac aggccaagcg atcacacaag tacccaaggc
      241 tatcgagccc tgggcgcaac gctccaaaaa gcgtcgacgt ggggagctgt ggtcattttc
      301 gctcatgact ggtggttcgg ccagtaccgt gagggtgtca acgagaaatc ggtccatgaa
      361 gggagcgaaa gggcttgggg caacataatg cagggaggcg tgggaaagga tggggtagcg
      421 cttgactgaa gcaggatgcg cgcgtaggtc ccggtccggt cgaaagactt cccacgatcg
      481 gagtcccgca tgcctggttg gctgagcgaa cttctcccca tttcgattct cctggtggtc
      541 gtggcgctcg ttctggcgcg gcttcccaag gtcaaagagg tggaacacag cgcggcgttt
      601 cgtcgtcgcc gtgtgttgaa ttggctcccg ctggggttgg catacgcgtt tctttatatg
      661 gggcggtaca acctcaaggt gagtcaacac gtattcgagc agatgaccaa tgccgatggg
      721 cagcaattga tgacgaacgc tggttttgcg accatttttg gggtgggcac ggcggtatac
      781 ggaatttctt ttttgttgaa tgggccgctg accgatcggt ttggggggcg aaaggcgatt
      841 ctcacgggga tgggaggagc ggccgcggcc aatgggttga tggctctgac catggtgatt
      901 ttccagcgtg gcggaccgat ggcagactcg gtgggaagga attttttgct tttattttca
      961 atactttatg cagccaatat gtactttcag agtttcgggg ccgtggcgat cgtgaaggtg
     1021 aatgctccct ggtttcatct tcgagagcgc ggcgtgtttg gggccatctt tggaattctg
     1081 atagccctgg gaatctattt cgcgttcgac tggagttata aaatcattag ttttttcggg
     1141 caggatccgc atcttgaaca ggcttcttcc cggctcacct gggtctttgc cgctcccgca
     1201 acgattctcg cggccttgtg ggttgtcgtg ggaatatggg tcaaaaatac accgggggat
     1261 gcgggccatg aggatttcga tacggcggat gcgtccagcg aagagcaggg caagaacctg
     1321 ggggcaggac aggtcatcaa gttgatgctg cgcaaccccg tggtcatgac cattgcaagt
     1381 gtcgaatttt gtaacggatt tctccgtcaa gccatcatgc aatggtatcc gacgttcgca
     1441 aaacatacga atggcgaact tgggcatatg gatggtttcg tgttcaaaaa ctggggattg
     1501 cttctttgta tcgcggggat cgtggggggc atggtggcgg gcgtgatctc cgaccacctt
     1561 tttcaatcgc gacgcggtcc cgtcgtatcc atcctgtatg gcggcatgat cgtggggagt
     1621 atcgtcctat ttttcgcgta tcagacatcc atggttggct ggatcgcggt gctgatgtcg
     1681 gtgctcgtca tcggcgtgca tggcatgctt tcgggcacgg cgagcatgga ttttggaggg
     1741 aaaaagaacg tcgggatcgt cgtggggatc atcgatgggt tcgtgtatgc ggggacagcc
     1801 atgatggcgt tgacctatgg gctgttattg cccgacgagc agctcaacga ggcggggaag
     1861 atcgtggggc catcgacgca agcgtcgaac tggtttgcat ggccggcggc gatgattccg
     1921 tttgcgatcg tgggtttcgt gttggcgctg cgggtctgga atgccaagcc acaatcgaag
     1981 gcggcgtcgg tgtcctgacg ttcggatttt gaaaatcggt gccgcgaagg catcgaagca
     2041 ttggggtgga gatacccggt caaggactca aggttggctt cgggcgcaac ggaaacccat
     2101 gtgggtgaga cggtgagagg ggttggcgct ttcgcgcgag ccgttgcgca gtcgattggg
     2161 attcatctcg ttccacgcgc cgccacgtcg catacggccg gagccgaact ggacggccgc
     2221 gcagttggag cagggcatgg gataggggtc gacgaatcga tccagcaccc attcggacat
     2281 gcttccagcc aagtccgcat gtccccagag gccatcgcca aggggtgagc gggaacccac
     2341 gtcgacgatg tcagcggcga cgcaaccggg ggagccatcg gtttcacaac cccagacggc
     2401 gtgatcgtgg tcgagggtat cgccccatgg gtacgttcgg tgttggctac cgccggaggc
     2461 ggcgaaattc cattcggctt ccgtgggcaa tctaccgccg gcccacgcgc aataggcaaa
     2521 tgcttcgtac caggtcacgc aattgatggg tcgtttttct ttggggccgg gggtatcggt
     2581 ccaagtttgg taggtcacat cgcaattcaa gctggtggtc aacgctccct tgtcggcaac
     2641 gagttcagcg ttccattcgg cgttccaccc tgaattcgca atcaacggat gcgcccccgc
     2701 gccaggagag ggcgggtttt tttgggttcc ccaacctgct tcgatgaatt cccgaaagcg
     2761 ccccacggtg acttcgaaag tatccaacgc gaagtcggcg accgtggcca cgtaggtatc
     2821 atccatataa tacacgccat cgtaagaacg gctgaacgag ccgccttgga catccgagct
     2881 tgcgcagcaa tcttgggtcg aggcattccc gcaattgttt cccgcccccg tggctccgct
     2941 atcgcaagcc g
//
LOCUS       DAOSOR010000671         2942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_33687_length_2942_cov_3.873225, whole genome shotgun sequence.
ACCESSION   DAOSOR010000671 DAOSOR010000000
VERSION     DAOSOR010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33687_length_2942_cov_3.873225"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PK710_23405"
     CDS             complement(<1..644)
                     /locus_tag="PK710_23405"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOT12747.1"
                     /translation="MRGSVPPCALMYVSILEEIVIILGLSMVVLLLGSRARIPPIVGF
                     LITGVVAGPHGLGLVEASAHVEVLAEIGVVLLMFTIGLEFSFKSLARIKKTVLLGGTT
                     QVLVTLGLGMLVAVLMGWKAQSSAFVGALVALSSTAIVLKLLQDRAQVETPHGQASLG
                     ILIFQDISILPMMLAIPLLAGTKESSDPPAWMIGKFLALIIGLIVSAKWLLPKI"
     gene            845..1024
                     /locus_tag="PK710_23410"
     CDS             845..1024
                     /locus_tag="PK710_23410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12748.1"
                     /translation="MHAERDLGFALGAADYIIKPFDAKALLRTLCCHRSKDRPAHVLV
                     VDNDEPRRQTLKKSS"
     gene            1015..1329
                     /locus_tag="PK710_23415"
     CDS             1015..1329
                     /locus_tag="PK710_23415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT12749.1"
                     /translation="MLVKEGWQVSKADNGRATLESLENWTPGLMLLNLMMPEGDGFSV
                     VEAMQTRPEWRSIPVVIVTVLDLSQADLDRLSGSIFKVLQKGSTASRTFWNSFVNACE
                     VE"
     gene            complement(1364..1906)
                     /locus_tag="PK710_23420"
     CDS             complement(1364..1906)
                     /locus_tag="PK710_23420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12750.1"
                     /translation="MTRLPHLSDNAQQERTDQGQCWWFGVNNGKHGHIRLMDIRPFML
                     GQEPEFAWPIGNSKPKGLYTHMKEGDRVLFWTGHGDEPEWGFIGTATISALHHDRIIF
                     GRARRFSTALTPYPKKYPHETDVIRSLERIFGRDYPPLGDVMRAVFGTRRRHPITVHP
                     IGENAFEEVMKLAQAPPNAR"
     gene            complement(1903..2493)
                     /locus_tag="PK710_23425"
     CDS             complement(1903..2493)
                     /locus_tag="PK710_23425"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOT12751.1"
                     /translation="MNSKPPHEVETSLYKLVFHPISDEEPSSVFADAAQDVSRDATEL
                     CIVGPYLGGAVLLPLVEGRKFRLITDLEACFGAGVDEAVLSLLSDHLDSIKNKAGVHA
                     KLVCSSKAALFGSANFTRQGFASRDELGCLIRDPQLLLIVQQWFEDLWLTSDAVDEKA
                     LSSVAKQVKERAVARQELGIATENPPDALAHGTWDG"
     gene            2608..>2942
                     /locus_tag="PK710_23430"
     CDS             2608..>2942
                     /locus_tag="PK710_23430"
                     /inference="COORDINATES: protein motif:HMM:NF025677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HOT12752.1"
                     /translation="MRPLPRHVAQEFQKYLRCGILAWGFTRAHCRSCEASMLVAFSCK
                     LRGVCPSCGARRASQTAANLVDRVLPRVALRQWVLSTHYELRVPMARCPALLSAVVRI
                     LCAEISRLMR"
BASE COUNT          626 a          793 c          873 g          650 t
ORIGIN      
        1 aagatttttg gaagcagcca ttttgcggat acgatgagtc cgatgatgag ggcgaggaac
       61 ttgccgatca tccatgctgg cgggtcggag gattctttgg ttccggcgag aagtgggatg
      121 gcgagcatca tcggcaggat ggatatgtcc tgaaaaatca ggatgccgag agatgcctga
      181 ccgtgcgggg tttcaacctg agcgcggtct tgcaacagct tcaagacgat ggctgtggaa
      241 ctcaaggcga cgagcgcgcc aacgaaagcg gaggattgcg ctttccaccc catgaggacg
      301 gcgaccaaca tgccgagtcc gagggttacg agcacttgtg tggttccccc gagcaggacg
      361 gtctttttga tgcgagcgag gcttttgaac gaaaactcga ggccgatggt gaacatcagc
      421 aagacgacgc cgatttcagc caacacctcg acgtgggccg atgcttcgac gagtccgagt
      481 ccgtgaggac cagcgacgac tccggtgatc aaaaaaccca cgatgggtgg gattcgtgcg
      541 cgggagccga gaagcagcac gaccatcgac aggccgagga tgatgacaat ctcctcgaga
      601 atggatacat acatcagcgc gcatggtgga actgagccac gcatgtgtca acgcgttctc
      661 accctccgtg ggcgatcagg cctggagatt tttgcgcgcc cctcgtagac ggacagggtc
      721 ctttgtccga agccggtcct ggtgattgcc ttttcgccgc ccccgcgacg tgacccggaa
      781 cttgtcttga cttgcgaaag ctgatcgatt tgtctatcgg catattcctg tcgttctcgt
      841 atccatgcac gcggagcgag acctgggatt tgcgctcggt gccgcggact atattatcaa
      901 gcctttcgac gccaaagcgc tgctgcgcac tctatgttgt catcgcagca aggaccgtcc
      961 tgcccacgtt ctcgtggtcg ataatgatga gccgaggcga caaacgctca aaaaatcctc
     1021 gtaaaagaag gttggcaagt ctcgaaagcg gataatggtc gcgcaaccct cgagtccctc
     1081 gaaaactgga cgcctgggtt gatgctgctc aacttgatga tgcctgaggg cgatgggttt
     1141 tcggtcgtgg aagcgatgca aacaagacct gaatggcgtt ccattcctgt cgtgatcgtc
     1201 actgtactcg atctatctca ggccgatctc gaccggttga gtggcagtat tttcaaagtg
     1261 ctgcaaaaag gaagtacagc ctcgcggacc ttctggaact cgttcgtcaa cgcatgcgag
     1321 gttgaatgaa gagccgagga cggaagattc gaggagagcg gaactaccgg gcgtttgggg
     1381 gtgcctgagc aagcttcatc acttcttcga aggcgttttc accaatcgga tggacggtga
     1441 ttgggtgtcg tctgcgggtt ccgaatacgg ctcgcatgac gtcgcccaac ggcggataat
     1501 cgcgtccgaa gatgcgttcc aagctgcgaa tgacgtcggt ttcatgtggg tactttttcg
     1561 gatagggggt caaggcggtt gaaaaccttc tggctctacc gaaaatgatc ctatcgtggt
     1621 gtagggcgct gatcgtggcg gttccgatga agccccattc gggttcatcc ccgtggccgg
     1681 tccaaaacaa tacacggtca ccttctttca tgtgtgtata caggcctttc ggcttgctgt
     1741 ttccgatggg ccatgcaaat tctggctctt gaccaagcat aaagggccgg atgtccatca
     1801 gccgaatatg tccgtgtttc ccgttattta cgccaaacca ccagcattgt ccttgatccg
     1861 tccgctcttg ttgggcattg tcggagaggt gaggaaggcg ggtcatccat cccaggttcc
     1921 gtgagccaac gcgtcgggcg ggttttcagt tgctatcccg agttcttggc gagcgactgc
     1981 acgttctttc acctgcttcg ccactgacga cagggctttt tcatccactg catccgatgt
     2041 gagccacagg tcttcgaacc attgctgaac gatgaggagc aactggggat ctcgaatcaa
     2101 acagccgagt tcatcccgac tcgcgaagcc ttgtcgagtg aagttggccg acccgaagag
     2161 cgccgccttg gaactacata caagtttggc atgcacaccc gctttgttct tgatggaatc
     2221 caagtgatcg gacagcaacg agagaaccgc ttcatcgaca cccgccccaa agcatgcttc
     2281 gagatcggtt ataaggcgaa actttcgacc ttccacgagg gggagcagca cggctccccc
     2341 gagataaggc ccaacgatgc atagctcggt cgcatcgcgg gagacatcct gagcagcatc
     2401 cgcaaatacg gaactgggct cttcgtcgct gattgggtga aaaacgagct tatagagcga
     2461 cgtttccact tcgtggggcg gtttggaatt caccataacc ataatgatac aacatggttg
     2521 ccagtagccc agcccgagaa ctccgtactg gttggggtcg tgcgaacttg actcgagacg
     2581 ttccttgcgg tggcaagcga agcgtacatg cgaccgctgc cacggcacgt cgcccaagag
     2641 ttccagaagt acttgcgctg cgggatcttg gcttggggct ttacgcgtgc ccactgccga
     2701 agctgcgagg catcgatgct cgtggctttc tcgtgcaagc ttcgcggcgt gtgcccgtcg
     2761 tgcggcgcga ggcgcgcaag tcagaccgca gcaaacctgg tggaccgagt cctgccccgc
     2821 gttgccttgc gacaatgggt gctgagcaca cactacgagc tgcgtgtacc gatggcacgc
     2881 tgcccggctc tgctcagtgc ggtggtgcgc atcctgtgcg cggagatttc ccgtctgatg
     2941 cg
//
LOCUS       DAOSOR010000672         2939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_33724_length_2939_cov_4.393204, whole genome shotgun sequence.
ACCESSION   DAOSOR010000672 DAOSOR010000000
VERSION     DAOSOR010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33724_length_2939_cov_4.393204"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1502)
                     /locus_tag="PK710_23435"
     CDS             complement(<1..1502)
                     /locus_tag="PK710_23435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12753.1"
                     /translation="MRNLLALVMAWVAISWTVVARADLIIRPGPQGGLGTWLVAGPLF
                     GTEKAMRFSGEQEIVLGATVMPGRDFRLLQAPDGQFDLQRELRAPSQAFALFGAVLRA
                     SSPTRVLLILGADDGVRVTVDDRVVLDKDLWRPPLHDDDAVAFDLSPGDHPLLIRLRQ
                     RSGPWKLSARLLDASNMAPCRTVQFVLPGASLDRVDESKLVQVDVGLDSFSDRYEPWV
                     HVMAQGGLPEGVELDVHASVVKTPPKSGEVLVFTSFLGKLAARGHRVHGLKARLLPLF
                     AEGLMADAADDTTEHPFEVRVRVGARETRVRRQGHAQIRRALGQIDRALLQMREHTHE
                     PQEAEVVASTLALSRERLAKYVSEGDDDLAATVDEAKAASTVARHVLDKQDPLPLIRG
                     PMRLAYPSPLDGKNRPFGVYVPPSWQEGKTYPLVVVLHGLNGLPMQMIRIYFGQDHPW
                     RRAPWEDRHVGSLAPLDAFVVSPSGFGNIGYREFGEMDVMHLVQWMKRTYP"
     gene            complement(1546..2025)
                     /locus_tag="PK710_23440"
     CDS             complement(1546..2025)
                     /locus_tag="PK710_23440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12754.1"
                     /translation="MTSRKLTAGGEVVAYCTKCRMDLNHRIVALVDGIPVKVECESCG
                     SHHKYRRPMEERATDKRERKTASSPRERSAPAPRTAGARAAAEAVQETAREKEWQSRI
                     AGKMADEFVKYTPKGSFEADALVHHFKFGDGYVVRVIDANKIEVMFRDGPRVLAQRI"
     gene            complement(2113..2613)
                     /locus_tag="PK710_23445"
     CDS             complement(2113..2613)
                     /locus_tag="PK710_23445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12755.1"
                     /translation="MAWDRYTKRMIRDDDDEPMEGTDGEAVRRRFVERLLPELIKRIV
                     DTGVVKLAEQPENLRHFVQELRVPKELGTYLLSQIDETKNGLYRVVAGEIRDFLEHTN
                     IADEVTRALTKLSFEIRTEIRFVPNDSAEGLFPKPEVQSEVAVKKTPAPKKRLEKTSR
                     FSKKRT"
BASE COUNT          610 a          943 c          774 g          612 t
ORIGIN      
        1 ggataggtcc gcttcatcca ctgcaccaaa tgcatgacgt ccatttcccc aaactcgcga
       61 tacccaatat tgccaaatcc gctcggcgat acgacgaacg catcgagggg cgcaagcgag
      121 ccgacatgcc tgtcttccca gggagcgcga cgccacggat ggtcttgccc gaagtagatc
      181 cgaatcatct gcatcggaag accgttcaat ccgtgaagca ccacgaccaa cgggtaggtt
      241 tttccctctt gccacgatgg cggcacatac acgccaaagg gacggttctt cccatccaac
      301 ggacttggat aagcgagcct catcggccca cggatgagcg gcaagggatc ttgtttgtcg
      361 agcacatgcc gggccaccgt cgacgccgcc ttcgcctcgt cgactgttgc cgccagatcg
      421 tcatcaccct cggaaacata cttggcgagc cgctcccgag ataacgcgag cgtcgatgcc
      481 accacctccg cctcctgcgg ttcatgggta tgctcgcgca tctgtagcaa cgcgcgatcg
      541 atctgcccca acgcgcgacg tatttgcgcg tgcccttgcc ttcgaacgcg tgtctctctt
      601 gcgcccacgc gcacgcgaac ctcgaacgga tgctctgtcg tatcgtcggc cgcatcggcc
      661 atcaacccct ctgcgaagag gggaagaagg cgtgctttca acccatgaac gcgatgcccc
      721 cgggcggcca gcttccctaa aaaacttgta aatacaagca cttcccctga tttcggcggc
      781 gtcttcacca ccgatgcgtg gacatccaat tcgacccctt cgggcaatcc tccttgcgcc
      841 atcacgtgaa cccacggttc gtatcgatcg gaaaagctat ccagccccac gtcgacttgc
      901 accagcttgg attcatccac acggtccaac gaggcaccgg gcaatacgaa ttgcacggtt
      961 cggcagggag ccatattcga cgcgtcaagc aagcgcgcgg aaagtttcca tgggcccgag
     1021 cgctgtcgca accgaatcaa gagcggatgg tcacccggag aaagatcgaa ggccacggcg
     1081 tcatcgtcgt gcaagggagg gcgccacaaa tccttgtcga gcaccacgcg gtcgtcgaca
     1141 gtaacgcgca cgccatcatc ggctccaagg atgagcaaca cccgcgtggg ggaggatgcg
     1201 cgcaaaacgg cgccgaagag agcgaacgcc tgggaaggag cgcgaagctc gcgctgcaga
     1261 tcgaattgtc catcgggtgc ttgaagcaac cgaaagtcgc gtcccggcat cacggtagcg
     1321 cccaggacga tttcttgctc gccggaaaac cgcatggctt tttcggttcc aaaaagcggt
     1381 ccggctacca accacgttcc caatcctcct tgcggtcccg ggcgaatgat aaggtcggcg
     1441 cgcgcgacga cggtccacga gatcgccacc cacgccatga ccaacgcgag caagtttcgc
     1501 atgcccctat ggcatcgaat cccttggata ttgcgagcaa ttagctcaaa tccgttgcgc
     1561 cagaacgcga ggaccgtctc gaaacatgac ttcgatctta ttcgcgtcga tgacgcgcac
     1621 gacatatcca tctccgaatt tgaaatgatg gaccaaggca tcggcctcga agctgccttt
     1681 cggtgtgtac ttgacgaatt catcggccat cttgccggcg atgcggcttt gccattcttt
     1741 ttctcgagct gtttcttgca cggcttctgc ggccgcacgt gcccccgcgg tccgaggggc
     1801 gggtgcgcta cgttctcgcg gcgaggatgc ggtctttcgt tctcttttgt ccgtcgcccg
     1861 ctcttccatg gggcgacgat acttgtgatg agacccacac gattcgcatt cgaccttgac
     1921 cgggatgcca tcgaccaacg cgacgatccg gtgattcagg tccattcgac atttcgtgca
     1981 gtaggcgacg acttcaccac cggccgtcaa ctttcgactt gtcatgcaga aggctcgatc
     2041 cgttgaagaa aagggcggca tccagggtga tgccgactgt aggttgcggc atatcgcggc
     2101 gtccggcaat gcctacgttc gtttcttgga gaagcggctt gtcttttcca ggcgtttttt
     2161 cggggcggga gtttttttga ccgcgacttc cgattgtacc tccggcttgg gaaacaggcc
     2221 ttcggcggaa tcgttgggca cgaaccgaat ctccgttcga atctcgaagg aaagtttggt
     2281 caacgcgcgc gtcacctcat cggcgatgtt ggtatgttcc aaaaaatcac ggatttcgcc
     2341 cgcaaccacg cgatacaaac cgtttttcgt ttcgtcgatc tgcgaaagca agtaggtgcc
     2401 caattccttg gggactcgca attcctgcac gaaatgacgt agattctccg gctgttccgc
     2461 cagcttcacc acgcccgtat ccacgatacg tttgatgagt tccggcagca gacgctccac
     2521 gaatcgcctc cggacagctt ccccatccgt gccttccatc ggttcgtcgt catcgtcgcg
     2581 aatcattcgt ttggtgtacc tatcccaagc cacgcggtca atcatcagcc ttcacgacgt
     2641 cgggccgtat ccagcggaag tcccctgatc cacgacagca atacgtcaag gttttcgata
     2701 cgaggctcac ttcaccaccg ttgccatacc aagatccagc ggatgctttt cgaaggtcca
     2761 acagggtcga tgcggcccaa tcctatcgaa agtaaggcca agcccctgcc aggaccccat
     2821 gaagccggct agttatggtg attcatcctc tcgatcgaaa acagtcatac cctttccccc
     2881 catccccggc ccttcccccc acacggtggc cccccatccc cggcccttgt tcctgcccc
//
LOCUS       DAOSOR010000673         2895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_34406_length_2895_cov_4.345070, whole genome shotgun sequence.
ACCESSION   DAOSOR010000673 DAOSOR010000000
VERSION     DAOSOR010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34406_length_2895_cov_4.345070"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PK710_23450"
     CDS             complement(<1..453)
                     /locus_tag="PK710_23450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12756.1"
                     /translation="MKIAWIGVGLMLVACSSDETADSGGGGDQGGSAAVGGSGGAAGG
                     GGGGGEGGVAGSGGMGGSGGVAGSSGAAGSGATAGSSGAGGTNVGGSGGEPRDYSLRP
                     FSDDSPWNIEIPPNATYLPNDPRTQRIRSFGAFGVNTVNWTIWTWYASD"
     gene            complement(858..1349)
                     /locus_tag="PK710_23455"
     CDS             complement(858..1349)
                     /locus_tag="PK710_23455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12757.1"
                     /translation="MKTQFVGLTVVGILSLCLGACDEKKAPASATSADKPAVEAPEKA
                     KEESPKPAAVPGDMKASKVEKAVAEWGKDDVKSAFEKSGWMVGSAVETTSSNLVSITV
                     VATKGEAKATVSYFRGGSDFWKKSLEKDGASIHEEEGILLGVLIKNDKEGSQTLLNSL
                     LGK"
     gene            complement(1676..2749)
                     /locus_tag="PK710_23460"
     CDS             complement(1676..2749)
                     /locus_tag="PK710_23460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12758.1"
                     /translation="MNSSDASVQDSSAEADAQNGDGDRKDANDGEVGPLIDGGQDSGM
                     EGGKDAGLEKDGDPDDDGSILDVAEEPEETIRCPKGSTEVFRDDFDGSEVDESKWNVV
                     DQGIGGGTFTQKTYMRRENVKVQDGNLVVSSWRHCENPRSNRNAPIHESRCPGTGDNY
                     YSGAWLKVKGGYAAGGKGSVGFRAKMPKPVPGSFPALWARNTEGGDLYTELDLIEMTW
                     DRPKNVPAAPDTFVVTTHFGAGAKYHAKGPAVGPFQGLTDSFHVWEVQWDAGTSPAIV
                     RYYYRDTADSPPQLLRETTVASSGFAGNVKETDFAEALRKAFRPYVDFAVLPETTWSV
                     SPDAAEIYAPDDLLVDCVIVCQY"
BASE COUNT          604 a          891 c          721 g          679 t
ORIGIN      
        1 atccgaagcg taccacgtcc agatggtcca gtttaccgtg ttgacaccga acgcgccgaa
       61 gctacgaatc cgctgcgttc gggggtcatt gggcaggtag gtcgcattcg gaggtatttc
      121 gatattccag ggcgaatcat ccgaaaaagg acgcaggctg taatcacgag gctccccacc
      181 gctgcctccc acgttggtgc ctcctgcgcc actgctgccc gcggtggccc cggagcccgc
      241 cgcaccgctg ctacctgcga ctccgccact tcctcccatt ccaccgcttc ccgcaacccc
      301 accttcccct ccacccccgc cgccacccgc cgcgccaccg cttcccccta cggcagcgct
      361 gccaccttgg tcccccccgc cgccagaatc cgccgtctcg tcggaactgc aagccaccag
      421 catcagcccc acgccaatcc acgcgatctt cataccatct ttggtacggc actgcggttt
      481 atctgtcaag gtgaataggt tgaaagatcc gttcatggat atcgaggagt gtcgagagaa
      541 accaccacgc tgtacgccat ttcccgaaga ctgacgtgca ggcgattcaa ggagtcattc
      601 gcggataatt gatagctaac tatatgaatt atcggtgttt gttcgtgagt tgccgattcc
      661 tgggaatcga tggcaagacc cgggaattgc caacatgcgc tcctcgatgc ctgacgttgg
      721 attgggtctg gaccctcgtt ggctttttcc cgagtgattc tcatgggata ggcgcagccg
      781 gacagagagc agccggaggc ttggtaggaa cgacttggtt ggaaaaaggt gcgtcctcta
      841 gcgggaatac gaatcgatca tttccctaaa agggagttga gaagggtttg ggagccctct
      901 ttatcgtttt tgatcagcac cccgagcaga atgccttctt cttcatgaat cgaagcgcca
      961 tctttttcga ggctcttttt ccagaagtcg ctaccgccgc ggaaatagct caccgtggct
     1021 ttggcttccc ctttggtagc gacgaccgta atgctgacaa gattcgagga cgtggtttca
     1081 acggcggagc ccaccatcca accacttttt tcgaaagccg atttgacgtc atcttttccc
     1141 cattccgcga cagctttttc tactttagac gctttcatat cccctgggac cgcagccggt
     1201 tttggggatt cttctttcgc cttttctgga gcttccacag cgggcttgtc cgctgaggtc
     1261 gccgaggcgg gagctttttt ctcgtcacaa gcgcccagac aaagggatag aatgccaacg
     1321 accgtgagtc cgacgaattg ggttttcatc cttcaaagcc ctttccatgg ataatgaaat
     1381 cggagcctac aggatacctg tggttgtcga tacctgtcca ttccttgata tttttcgtaa
     1441 ccgtgtcgtg aatcggactt tcgtcaccct tgggaagtga ctcgtttccc gccacgatcg
     1501 tcatcgattc cccacgaatt gggccgactt gtgcgaactg cgccccaggc cgaacatcga
     1561 accgtgaaat gacgacatgc ggcaccttct tctggtcttc gaattcgaaa ggcccagagt
     1621 ccgcctggat gccatggata aggagcagtt agaacaagat aattagccct gtccttcagt
     1681 attgacaaac gatgacgcaa tcgaccagca aatcgtcggg agcatagatt tccgcggcat
     1741 cagggcttac cgaccaggtc gtttccggca acaccgcgaa gtccacatag ggacgaaacg
     1801 ctttgcgcaa cgcctcggcg aagtcggttt ctttcacgtt gcccgcaaaa cccgaggagg
     1861 caaccgtcgt ttcccgcaac aactgaggag ggctatccgc ggtatcacga taataatacc
     1921 gaacgatagc ggggctggta ccagcatccc attgcacttc ccagacgtga aaactatccg
     1981 taagtccctg gaaaggtccg acagcgggac ctttggcgtg gtatttggcc cctgctccaa
     2041 aatgcgtcgt gaccacgaag gtatccgggg ccgcggggac gtttttcggc cgatcccacg
     2101 tcatttcgat caaatcaagc tcggtataca gatcccctcc ttccgtattt cgtgcccata
     2161 gcgccggaaa cgaacctggc accggcttgg gcatcttggc tcggaaccct acggacccct
     2221 ttcccccggc cgcatacccc cctttcacct tgagccacgc tccagaataa tagttgtctc
     2281 ccgtgccagg acaacggctt tcgtgaatcg gggcattccg attcgaacgg ggattctcgc
     2341 aatgccgcca cgacgaaacc accagattcc catcctgaac cttcacgttt tctcgacgca
     2401 tataggtctt ttgcgtaaat gtcccgcctc cgatcccctg atcgacgaca ttccatttcg
     2461 attcgtccac ctcactgcca tcgaaatcat cgcgaaaaac ctccgtgctg cccttcgggc
     2521 accgaatcgt ctcttccggt tcttcagcga catcgaggat gctaccatcg tcgtcaggat
     2581 caccgtcctt ctccaaaccc gcatcttttc cgccttccat gccggaatcc tggccgccgt
     2641 cgataagagg gccgacttca ccatcgttgg cgtcttttcg gtccccatcg ccgttttgag
     2701 cgtcggcttc ggcgctggaa tcttgtacgg aagcatcgga actgttcatg tcatccgagg
     2761 tagaagaact cgaacacgac aggggcagac ccgcgctgac cgaaaataga aacataccga
     2821 cccctatgct tcgcatatac cttgttcgca ggttcatttt gctttctttg cctccggagc
     2881 ggttgatggt agggg
//
LOCUS       DAOSOR010000674         2895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_34412_length_2895_cov_3.904225, whole genome shotgun sequence.
ACCESSION   DAOSOR010000674 DAOSOR010000000
VERSION     DAOSOR010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34412_length_2895_cov_3.904225"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PK710_23465"
     CDS             <1..599
                     /locus_tag="PK710_23465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12759.1"
                     /translation="FASSTGGVGTCSPSTFKHVPNEFRSDIAGFVLSQAQAKGGGVLL
                     FHDIHTYTVSKLDTILSRLEQNGFKFVPLSDTSTFPLLNGSAPKPSAWVGTPCTDDSQ
                     CNFTASGSSGFCWTYDDSNLGFCSLECNGYCPDKSGTAPTFCTSVDGGQTGQCVSKAS
                     EANHQCSDLPGTDPVSAQRFVGTSTASAATALVCLPSP"
     gene            796..2445
                     /locus_tag="PK710_23470"
     CDS             796..2445
                     /locus_tag="PK710_23470"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOT12760.1"
                     /translation="MMKGRLAEKITDLTLELTSVRSIVGTAGEVDIANRVHQIFRKMK
                     YFQQHPENLHFVPVPHDPLGRKSVLAIVKGEKRQNKKAIVLIGHIDTVGTSDYGLLEP
                     YATKPLELAEKFKEASLTDEVRTDLESGNYLFGRGIFDMKCGVSTLMAIIESVTEDIQ
                     GFEGNLVFAAVCDEEGNSSGMLSVVPELIRLQAQEGFEYQALLDTDYSAPRFAGDTTK
                     YVYIGTVGKLMPSFYVVGSEAHGGDPFKGLDSNHLSSAIMQEIDFNMKYSDEAEGEVT
                     VPPISLKQTDLKQEYSVQTPKTGYLYFNYGTHASGPHQVLDKVMAGAKLAFQNLVDRL
                     NEEHETYCKASGIPFHKLPWTARVLSYAELYDRVVAEKGVAAKEVLETRKNELLADPN
                     VDERLFALRMVETLHNLWSDNDPVVIAYFSPPYYPHVFVKGDSPLEKKLLRVVDELIQ
                     QTYASHHVKMKRFYPYISDLSYASAPRDPSAIEALQANMPGFGTKYVLPLKEMQDLNL
                     PVINIGPFGKDAHRYTERLEKQYSFDIVPGLVRETIIKLLQ"
BASE COUNT          685 a          836 c          750 g          624 t
ORIGIN      
        1 gttttgcgag ttccacgggc ggggtaggca cctgttcacc gagcaccttc aagcatgttc
       61 ccaatgaatt tcgctctgat atcgcggggt tcgtattgtc ccaggcccag gccaaggggg
      121 gaggcgtgtt gctattccac gacatccaca cctatacggt cagcaagctc gacaccatcc
      181 tttcgcgcct ggagcagaac ggcttcaagt tcgtcccgct gagcgacaca tcgacgtttc
      241 ctttgctcaa cggctcggca cccaaaccgt cggcatgggt cggcacgccc tgcacggatg
      301 actcccaatg caacttcacg gcatcggggt cgagcggctt ctgctggact tacgacgatt
      361 ccaacctcgg attctgctcg ctcgaatgca acggttattg tccggacaaa tccggtaccg
      421 cacccacgtt ttgcacaagc gtcgatggcg gccagacggg gcagtgcgtg tcgaaggcaa
      481 gtgaagccaa ccaccaatgc agcgatctac ccggtaccga tcctgtttcg gcacagcgtt
      541 tcgtgggaac gagcacggca tcggccgcga ccgccctggt ctgcctgcct tcgccctaat
      601 cgatagtcac catcctggcc tcatcgagcc ccgctcgctt tgtcggggaa gtccggggcg
      661 gctccgccac cgaagtggca attcacgatt cccatctatg ttccaagcct tccaacacgg
      721 gcagctttcc atggtcttgg cttgcgaaag ccccctgctt gtcctacaga cctgctccaa
      781 cgaccgaagg agaccatgat gaagggcaga ttggctgaaa aaataacgga tctgactctg
      841 gagttgacga gcgttcgaag catcgtcgga accgcgggag aagtcgatat cgccaaccgg
      901 gtccaccaaa tctttcgaaa aatgaagtat ttccaacagc atccggaaaa tctccatttc
      961 gttcccgtcc cccatgatcc cctcggaaga aaaagcgtcc tggctatcgt caaaggggaa
     1021 aaacgccaaa acaaaaaagc gatcgttctc atcggccata tcgatacggt ggggacgtcg
     1081 gattacggcc tgctcgagcc gtacgcgacg aaacctcttg agttggcgga gaaattcaaa
     1141 gaagcatcgc taaccgacga ggtgagaacg gatctggagt ccggaaatta tctctttggc
     1201 cgagggatat tcgacatgaa gtgcggggta tccacgctca tggctatcat cgaatcggtg
     1261 accgaagata tccaggggtt cgaaggcaat ctcgtatttg cggcggtatg cgacgaagaa
     1321 ggcaattcct ccggtatgct gtcggtggtg ccagaattga tacgactgca agcgcaggaa
     1381 ggattcgaat accaggccct gctcgacacc gattattccg cccctcgatt tgcgggagat
     1441 accaccaagt acgtctatat cggaaccgtg ggtaagctga tgccgagctt ttatgtcgtg
     1501 ggttccgaag cccacggcgg agaccccttc aaaggattgg actccaacca cctgtcgtcc
     1561 gccatcatgc aagaaatcga tttcaatatg aaatacagcg atgaggcgga aggagaggtg
     1621 accgtccccc ccatctccct caagcagacg gatttgaaac aagagtattc cgtgcaaacc
     1681 ccgaaaacgg gatatctata tttcaattat ggcacccacg caagcggccc tcaccaggta
     1741 ttggataagg tgatggcggg agccaagttg gcattccaaa acctggtgga tcgtctgaac
     1801 gaagaacatg aaacctattg taaagccagt ggaattcctt ttcacaagct tccgtggacc
     1861 gcgagggttc tatcctacgc cgagctgtac gaccgcgtcg tcgcggaaaa gggcgtggcc
     1921 gcgaaagagg tattggaaac ccgaaagaat gagttgctcg ccgatccaaa cgtcgacgaa
     1981 agacttttcg cccttcgtat ggtggaaacc ctgcacaacc tgtggtctga caatgatccg
     2041 gtcgtcattg cctacttctc gcccccctac tatccgcacg ttttcgtcaa aggcgattcc
     2101 cccttggaga aaaaactcct tagggtcgtc gacgaactca tccaacaaac gtacgcctct
     2161 catcacgtca aaatgaaacg attctatccg tatatctctg atctgagtta cgcttctgcc
     2221 ccccgagacc cctcggccat cgaggccctt caggccaata tgcccggctt cggaaccaag
     2281 tacgtgttgc cgttgaaaga aatgcaagac ttgaacttgc ccgtcatcaa tatcggacct
     2341 tttggcaaag acgctcatcg ctatacggaa cggctcgaaa aacaatactc cttcgacatc
     2401 gtgccaggcc tggtgcgcga gacgatcatc aaacttctac agtaataccc gccatgcctg
     2461 ctgcgagctg catccccgcg cttgcattcc cccaggctcg tcttgcttgt taatcctcca
     2521 accttcccat agcgatggcg ggaatcggaa acgttctggc gacgacccca cgaaaaagtc
     2581 gctctcccta tcggccgctt tggcgtaacc catggaatac actgaaggat gcgtttgccg
     2641 ggtggcagtc gtcctggggc gtccttatgc tggtcttcat cttccaatcc aggtccgttt
     2701 cgaaatcata ggtcgtatgc catggctccc aatacgcagg aaacactgaa gacaatcgcc
     2761 cgcgagagaa tccttttgct cgacggggcg atgggcacga tggttcaacg cttcgaattg
     2821 gacgaatcgt cgtttcgtgg aacgcgtttt gccgaccatc ccagggacct gaagggcgat
     2881 aacgatctgc tggtg
//
LOCUS       DAOSOR010000675         2880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_34647_length_2880_cov_3.926018, whole genome shotgun sequence.
ACCESSION   DAOSOR010000675 DAOSOR010000000
VERSION     DAOSOR010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34647_length_2880_cov_3.926018"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="PK710_23475"
     CDS             <1..1197
                     /locus_tag="PK710_23475"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibro-slime domain-containing protein"
                     /protein_id="HOT12761.1"
                     /translation="KSRCGDGIILPGDSNEQCDDGNNQSGDGCSATCTVEPGFTCAPI
                     VVGGGPTMTLPIVLRDFHDTHPDMEKYLGTESGIVKPLLGADRKPEYAHGTSSSLTVT
                     SEATFNQWYRDVPGVNATALQTLELTRLATGEYQYDNSSFFPLDGQLFGNEGRAHNFH
                     FTSEVRYWFEYKGNEILLFTGDDDVWVFLAGKLAVDLGGVHGALPGGIELNATTAAAF
                     GLTLGQVYEIAVFQAERHTTQSNYRLTLSNFDNVRSKCDWICGDGIVTKYEACDDGVN
                     DGSYGSCMPGCKLRAPHCGDGIVQTSEGEECDDGLNLSVYGGCAPGCKLGGYCGDGVV
                     DSLFGEQCDDGINAGGYGECAPGCILGPRCGDGIMQTEEGETCDDGNRVSGDGCSANC
                     NREAPH"
     gene            complement(1248..>2880)
                     /locus_tag="PK710_23480"
     CDS             complement(1248..>2880)
                     /locus_tag="PK710_23480"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOT12762.1"
                     /translation="EDALAAALYLMSGVRAVAAGRVGRDKLLPVQIPRLAMTGEHLDQ
                     VVAAIVSLYRQREKVAPLEPLSVGTWRDQMRYRSVFADLTPYEFDTFPYLIHTVEHVG
                     TLTREERLKAIRAAGYNTFLLRSADVAIDLLTDSGTSAMSTDQWAAYDAAHASSANSS
                     EFEHFVEVLQDIYGYQQIIPTHQGRAAEHILSQIMIKPGQLVPGNMYFTTTKEHQELA
                     GGHFIDVIVDEAHVPQSDCLWKGNIDLGKIDKAVKERGAEGIAYISFELAVNMAGGQP
                     VSMDNMKEVYAYCSARKIPVFYDATRAVENAYMIQQRDPRYRTTKIRDILREMMLYGD
                     GCTVSGKKDFLINIGGILAFRDDKELAARAQELLRVYEGSVASGGLATADLAVIAQGA
                     QEMLDDRYIRSRVRQTQLLGSMLMEAGVPIVTPPGSHAIFLDAKRFLPHVPQEEYPAQ
                     RLAAEIYVETGVRVMERGNVSSGRDENGKNYCPALELVRLTISRRVYTNDHMRAVADG
                     IIRLYQRRNEIKGLRFVYEPAHLRFFQGRFEPIEG"
BASE COUNT          647 a          786 c          808 g          639 t
ORIGIN      
        1 aaatcacgtt gtggcgatgg gatcatcttg cctggagatt ccaacgagca atgcgatgac
       61 ggcaacaatc aatccggcga tggttgttcc gcgacatgca cagtcgaacc cggtttcact
      121 tgcgcgccca tcgtggttgg cggcggtcca accatgacgc ttcccatcgt tcttcgggat
      181 ttccatgaca cccatccgga catggaaaaa taccttggga ccgagtcggg tatcgtcaaa
      241 cctctgcttg gggccgatcg aaaacccgag tacgcgcatg gcacctcttc gagtctcacc
      301 gtgacgagcg aggccacgtt caaccaatgg tatcgcgacg tgccaggcgt gaacgctacc
      361 gcattgcaaa cgctggaact gacacgtctg gccacgggcg aataccaata cgacaacagc
      421 agttttttcc cgttggatgg tcagctattc ggcaatgaag gacgagccca taacttccac
      481 ttcacgagcg aggttcggta ttggtttgaa tacaaaggca acgagatact gctgtttacg
      541 ggcgatgacg atgtctgggt atttttggcc ggaaagttgg cagtggattt gggcggggtt
      601 catggcgctc tacccggcgg gattgaattg aacgccacga ccgcggcagc ctttgggctc
      661 acactcggac aagtatacga aatcgctgtg tttcaagcgg aacggcatac cacccaatcc
      721 aattatcgtc tcacgttatc caacttcgac aacgtgcgaa gcaagtgcga ttggatttgt
      781 ggcgatggga tcgtcaccaa gtacgaagcc tgtgatgacg gcgtcaacga tggttcttac
      841 ggaagctgta tgcccggctg caagcttcgt gcacctcatt gcggagatgg aatcgtgcaa
      901 acgtccgaag gagaggagtg cgatgacggt ttgaatttga gtgtttatgg cggatgcgcg
      961 ccgggatgta agttgggagg atattgtggg gatggggtcg tggatagttt gttcggcgag
     1021 caatgcgacg atggaatcaa cgctggagga tatggggagt gtgctcccgg atgtatctta
     1081 gggcctcgtt gtggggacgg gatcatgcag acggaagagg gcgaaacgtg tgatgatggc
     1141 aaccgcgttt caggggatgg ttgcagcgcc aattgcaaca gggaggcccc ccactgacag
     1201 agagcgtgtc agaatcgtcg catcgtcaag aggatgagga gcgttgctta tccttcgatg
     1261 ggttcgaatc ggccttggaa aaagcgaaga tgggcgggct catacacgaa acgcaagcct
     1321 ttgatctcat tgcgtcgctg gtatagacgg atgattccat cggcgaccgc tcgcatgtga
     1381 tcgttggtgt acacacgccg cgaaatcgtc aagcgaacga gttcgagagc tgggcaataa
     1441 ttctttccgt tttcatcgcg tccgctcgag acattgccgc gctccatgac tcgaacgcca
     1501 gtctcgacgt agatttcggc cgcgagtcgc tgcgctggat actcctcttg cggaacatgc
     1561 ggaaggaaac gcttcgcatc aaggaagata gcgtggcttc caggaggcgt aacgatcggc
     1621 acccccgctt ccatcagcat cgatccaagc aattgcgttt gtcgaacgcg cgatcggata
     1681 tagcgatcgt cgagcatttc ctgcgctccc tgcgcgatga cagccagatc tgccgtggca
     1741 agacctccac tcgccacgct accctcatac actcgcaaca gctcctgcgc gcgagccgca
     1801 agctccttgt catcgcggaa cgccaagatg ccaccgatgt tgatgaggaa atccttcttc
     1861 ccgctgacgg tgcatccatc cccatacagc atcatttctc gaaggatgtc gcggattttt
     1921 gtcgtgcggt atcgtggatc tcgctgctgg atcatgtatg cgttctcgac agcgcgcgtg
     1981 gcgtcataga aaacgggaat ctttctggcg ctgcaatacg catagacctc tttcatattg
     2041 tccatgctca cgggttgccc gccagccatg ttcaccgcaa gttcgaagga aatataagcg
     2101 atcccctccg ctccccgctc cttcaccgct ttgtcgatct tgccaagatc gatgtttcct
     2161 ttccacaagc agtcgctctg cggaacatga gcctcatcga caatcacatc gatgaagtgc
     2221 ccacccgcaa gctcttgatg ctctttggtc gtcgtaaaat acatattacc cggaacgagt
     2281 tggccgggtt tgatcatgat ctgcgaaagg atatgctcgg cagctcgtcc ttgatgcgtc
     2341 gggatgattt gctgataccc atagatatcc tgcaacacct ccacaaaatg ctcgaattcc
     2401 gaactattgg cggatgacgc atgagccgca tcatacgctg cccactgatc ggtgctcatc
     2461 gcagacgtgc ccgagtcggt gagcaagtcg atggcaacgt cggcggaacg aagcaaaaac
     2521 gtgttatatc cagccgctcg gatcgccttc agacgctctt cccgggtaag cgttcccacg
     2581 tgttccaccg tgtggatgag ataggggaac gtgtcgaatt cgtagggagt caggtccgcg
     2641 aataccgaac gataacgcat ttgatcgcgc caggttccca cggatagcgg ctcgagaggc
     2701 gccacttttt cgcgttgacg atacaagcta acgatagcgg caaccacctg atcgagatgc
     2761 tcgccggtca tcgccaagcg cggaatctgc acgggaagca gcttgtcccg tccaacacgc
     2821 cccgcggcta ccgcacgcac accgctcatc aagtacaacg ctgctgccaa cgcatcttcc
//
LOCUS       DAOSOR010000676         2876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_34720_length_2876_cov_4.214463, whole genome shotgun sequence.
ACCESSION   DAOSOR010000676 DAOSOR010000000
VERSION     DAOSOR010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34720_length_2876_cov_4.214463"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(353..1159)
                     /locus_tag="PK710_23485"
     CDS             complement(353..1159)
                     /locus_tag="PK710_23485"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOT12763.1"
                     /translation="MALAICLTLAWGLAIPSAHAQQAGIRSHRGWLGVLMQESPDGRV
                     LVDEVIRGSPAERAKIQRGDLIEQVEGVAVHSPKQMAQQVGRFGAGAVVRVQFLRKGT
                     PLTVMPRLEPYPSNEQLLRLQWLGRPAPALQGLLATDGSPGPILDSFRGKVLVLDFWA
                     SFCIACRVSTVSMNGWHQRYAGQGLEIVGIAAEPPEVVEQASRRFAMRYQAYADPDMT
                     TTASYRVQELPSVVIIDRKGIIRDVATGYAPERMHQLETLIQRLLREPNP"
     gene            1428..1772
                     /locus_tag="PK710_23490"
     CDS             1428..1772
                     /locus_tag="PK710_23490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12764.1"
                     /translation="MQVIGTFWSRGHGTGHWQSEARVNGGTEQAMASLVRPTDSMPVG
                     SLLVQSHSQNGTKLGYFAMKKREPGYFSEGGDWEYVVVSRDGRVESRGKIESCARCHA
                     QAPVDYLFRLTP"
     gene            2338..2411
                     /locus_tag="PK710_23495"
     tRNA            2338..2411
                     /locus_tag="PK710_23495"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2372..2374,aa:Arg,seq:acg)
     gene            complement(2430..>2876)
                     /locus_tag="PK710_23500"
     CDS             complement(2430..>2876)
                     /locus_tag="PK710_23500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12765.1"
                     /translation="DGGLLGEREYDLSRVIQGATVRHSPSGLQLELGESSSAAWPPSS
                     MLTNPRISFRMQATPAVVRQGKHPIAMDAVAFEILIPHRPMTTSEIIHGSIVLDVDML
                     GLELGEPVRFVLETDVGPIISKQHLRLEVNTFVRDVVKIGPSIPSD"
BASE COUNT          665 a          784 c          833 g          594 t
ORIGIN      
        1 ccagcgagcc gtcttccacg aacgttcccg catccacgag catggatatg cgttctcgag
       61 cgaagttttt gccttgttct tcgtttttgc ggtgagaggc ctcgggacct ccgcgctcga
      121 tgcgctcgag caattcaacc agcgacaagc ttgcaaccat gatgaccgtg ttctagctgc
      181 cttatgacaa ggcgtcatgt ggtggttgag tgaaggttcg tgcagaattt ctccacgagc
      241 cattcacgga tggtgccatg acaacgaagg acccccatcg gcccatggat agcagcagat
      301 gcgagccatg acaaaccgca ggcattcgcc gttttttgtg cccaggcaac aattaggggt
      361 tcggttccct aaggagacgt tggatcagcg tttcgagctg gtgcatgcgc tcaggcgcat
      421 accctgtcgc cacatcgcgt atgattccct tacgatcgat gatcacgacg gagggcaatt
      481 cttggacacg gtaggaggcg gtggtggtca tatcggggtc ggcataagct tggtaccgca
      541 tggcgaagcg acgggaggct tgctccacga cctcgggagg ttccgcggcg attcccacga
      601 tctcgagccc ctggcccgca tagcgctgat gccaaccgtt catcgaaacc gtgctgacgc
      661 gacaagcgat acagaaggaa gcccaaaagt ccaagacaag gactttgcct cgaaatgaat
      721 cgagaatagg acccggactg ccatcggtgg cgagcaatcc ttgtaaggcc ggtgcgggtc
      781 tgccaagcca ttgcaaacgc agcagttgct cgttggatgg atagggctcg agacgaggca
      841 tgaccgtcag gggcgttcct tttcgcagaa actgcacgcg aaccacggct ccggctccaa
      901 aacgaccgac ctgttgcgcc atctgtttcg gcgaatgcac cgcgacgcct tccacctgct
      961 cgatgaggtc gccgcgctga attttggccc tttccgccgg tgagccgcgt atcacctcat
     1021 cgacaagcac tctgccgtcg ggactttctt gcatcaggac cccaagccat ccacgatggg
     1081 agcggattcc cgcctgctga gcatgcgccg acgggatagc caggccccaa gcgagggtca
     1141 gacaaatcgc cagtgccaca cggaccagga cgcccttccg tagcctgtgg tatgtaaaag
     1201 aaatcacgat attggtcatg actcacttgc ccctagatgt cgagcgaact gtacgacagc
     1261 acgtccacct cattgtcgca ttgtctgtcc tcactgcatg ggcctgccac ccgaacccgt
     1321 cgttcatacc tggtttttcc gacgcttcac gcccttcgga ggtaccacca tcggccgtta
     1381 ccacccacca gcccgccgtc tgggaacact ttcatggctt gcatgacatg caagtcatag
     1441 ggacgttttg gtcccggggg cacggtacgg gacattggca aagcgaagca cgcgtcaacg
     1501 gggggactga acaggcgatg gcttccctgg tgcgacctac tgacagcatg ccggtgggct
     1561 cgctgctcgt gcaaagccac tcgcaaaacg gcacaaaact aggttatttc gcgatgaaaa
     1621 aacgcgagcc gggatacttt tcggaagggg gggattggga gtatgtcgtg gtgagccgtg
     1681 atggcagggt ggaaagccga ggcaaaatcg aaagctgcgc ccgttgccac gcacaagctc
     1741 ccgtcgatta tctatttcgc ctgactcctt gatgaacgat cggcaggggc ggtaaggcga
     1801 cacaccgcgg catgcgtgcc gccgtggccg tttcatcgag gcaaaaccgt tgaggtgggg
     1861 aaagaagaag ggataagtca gacaaaagca gcgactagga ataaggattc gtgaacaagc
     1921 gtgtaataag cgatgtcaag ggaagaaatt gaaaacaagt taagaagact aaggaaaaaa
     1981 agaaaaggat taaggaagag aactcggcaa tctgggaggc aagaagaaga taagtgagat
     2041 taccgaggtg caaccaccaa gacgttgcgg ctgtcttagc agcatccgcg ccaactgttg
     2101 taatatgtgt aaattcaaat acttacgttg gtgacgcgcg agtgaagcgc tcgcggtccc
     2161 gacagcggtg tcatagcgac caataaggtg tgcattattt gcgggcctga ttgtcttgaa
     2221 cacgaatccg ttctcgatga aagccgattt catgctcgtc ggttttccct gtcggccctt
     2281 cgaaatgtcg cttgacatct ctacccccca caagctatct cccgcgtccg ttcgtcggcg
     2341 cccgtagctc agttggatag agcattggtc tacggaacca agggtcgcag gttcgaatcc
     2401 tgctgggcgc gctagtgacc gcacacgtgt cagtctgacg ggattgaggg gccgatcttg
     2461 acgacatcgc gtacgaaggt attgacctcg agccgcagat gctgcttgga aatgattggc
     2521 cccacatccg tttccaagac aaaacgtacg ggttcgccca gttcgagccc cagcatatcc
     2581 acatccaata cgatgcttcc atggatgatt tccgacgtgg tcatcgggcg atggggaatg
     2641 aggatttcga atgcgactgc atccatcgca atgggatgtt ttccttgtcg gacgacagcc
     2701 ggggtggctt gcattcgaaa agaaattctt gggttcgtga gcatgctcga ggggggccaa
     2761 gccgcggaag atgattctcc caactcgagt tggaggcctg acggtgaatg acgaaccgtg
     2821 gcgccttgga tgacgcgaga caaatcgtat tcccgctcgc cgagcaaacc gccatc
//
LOCUS       DAOSOR010000677         2874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_34750_length_2874_cov_4.275630, whole genome shotgun sequence.
ACCESSION   DAOSOR010000677 DAOSOR010000000
VERSION     DAOSOR010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34750_length_2874_cov_4.275630"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="PK710_23505"
     CDS             complement(<1..1258)
                     /locus_tag="PK710_23505"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT12766.1"
                     /translation="MTVRIGQLLDGKYRVVRVLGKGGMGAVYEGENTLISRRVAIKVL
                     LPELLKKRDVATRFELEAQAAGRIQNDHINEVLDLGTLEDGARFIVLEFLEGETLKER
                     VKRVGRLTPMQAVHIVRQVLVGLSAAHRAGIVHRDLKPGNVFILWEKLGQRDFVKIID
                     FGVSKFLMSQESQVLDESHANAVLGTPHYMAPEQLRRSGTVDARADLYSVGIILYKAV
                     TGHVPYHAQSVKELLVKLTTTAPTPATRWVAGIDAGFLRILDKATQRDVERRFQSADE
                     FLAELEQWRLEMAANELPYFTEDGLMSTMHNVSLKRGSSEVSVPPVHLSSSSVDSISI
                     HELPDVSLPAKPATMAEQPAHAVQPQPIMPAQPVARSSWMGKGVAVLLVVTAMGGVGV
                     GAWYLSRTFRPPPTSSRQRWRVKSKAS"
     gene            1399..2421
                     /locus_tag="PK710_23510"
     CDS             1399..2421
                     /locus_tag="PK710_23510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12767.1"
                     /translation="MQFAVLKRSILTGSLLLAMVASASLAEGQLTDAQRKAARTAANE
                     GLQAFESGDFPHAIEQFEQANQIIRAPTHTLYIARAYAKLGQWLKARELYSEIVQEPL
                     AAEAPPAFVQARKDAENELETLEALIPRITIEISGHADETVQVTMNDKPIAAEAIGVA
                     QRVEPGTYLFRATSGQFRARSVKITIAEKERKTVNLELEAEPPPSPSPTEPVAPVSTS
                     ANQTTVYPYLGYGALGIGVAGIALGFYFNKQHADRLEEGDSLFDKCNPRVCTVEERNR
                     ISDIDDEASSNKQMSTISFVAGGVAIVGGAALLLFAPSQSGSSDTSSSVRPWLGLNSA
                     GISGSF"
     gene            2533..>2874
                     /locus_tag="PK710_23515"
     CDS             2533..>2874
                     /locus_tag="PK710_23515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12768.1"
                     /translation="MNRFYRYASHSAAFMSLAAALGACNPSKPSPSQTVTSASSVPSG
                     SVGATHAKQEANALLNTYLLELAKLEKAQALAYWTAANSGKKEDFDAAARAELELKKL
                     HSSADRYRQIVS"
BASE COUNT          640 a          869 c          763 g          602 t
ORIGIN      
        1 tggacgcttt tgactttacc cgccaccgtt gtctgctgga tgtcgggggc ggacgaaaag
       61 ttctcgacag ataccaggcg ccaacaccaa ccccacccat cgcggtcacg acaagcagca
      121 ctgccacgcc ttttcccatc cacgacgagc gagcgacggg ttgcgctggc atgatgggct
      181 gtggctgaac cgcatgggct ggttgttccg ccatggttgc gggtttggcg ggcaaagaga
      241 catcgggaag ctcgtgaata gaaatcgaat cgacgctgga agaggataaa tggacgggcg
      301 gcacggagac ttccgaactt ccccgtttga gcgacacgtt gtgcatggtg gacatgaggc
      361 cgtcttcggt gaagtacggc agctcattgg cagccatttc caaacgccat tgctcgagtt
      421 cggcaaggaa ttcatccgcg gattgaaagc gacgttcgac atcccgctgg gtcgctttgt
      481 ccaatatgcg caaaaaacca gcatcaattc ccgcaaccca acgagtggcg ggggtgggag
      541 cggtggtcgt aagcttgacc aacagttctt ttaccgactg cgcatgataa gggacgtgtc
      601 ccgtgacggc tttgtaaagg atgataccga cggagtacaa gtctgcccgg gcatcgaccg
      661 ttccacttcg tcggagctgt tccggcgcca tatagtgcgg tgtgccgagc acggcattgg
      721 cgtgggattc atccaagact tggctttctt gactcatcaa gaacttggag acaccaaagt
      781 cgatgatttt cacgaagtcc cgctgcccga gtttttccca aagaatgaat acgttccctg
      841 gcttcaaatc acggtgaacg atcccggccc gatgggcggc ggacaacccg acgagcacct
      901 gacgaacgat atggacagct tgcatcgggg ttaggcgacc gacacgtttg actcgttctt
      961 tgagggtttc tccttcgagg aattccaaca cgatgaaccg ggcaccatct tccagcgtgc
     1021 cgaggtcgag aacctcattg atatgatcgt tctgaattcg tcccgccgct tgcgcctcga
     1081 gttcaaaacg cgtcgcaaca tcgcgttttt tcagcagttc cggcagcaat accttgatgg
     1141 ccacgcgacg agaaatcagg gtgttttccc cctcatacac ggctcccatg cccccttttc
     1201 cgagcacgcg aaccacacgg tatttcccgt cgagaagctg accgatacgc acggtcatcg
     1261 cagcaccata tcctcgatcg cttgtatcac tcgcgttgcg tggccgcaag ccaacggctg
     1321 ccgttagacc cctgtcttcg cctctttcct ggattcaaaa taggaagcgg cgtaagagcc
     1381 tgctaccatg cgagcaggat gcaattcgcc gtattgaaac gttccattct cacaggatcg
     1441 ctactgttgg ccatggtcgc ttccgcttct ttggcggagg gacaactcac cgatgcgcag
     1501 cgcaaggccg cacgaaccgc cgccaacgaa gggttgcaag ccttcgaatc gggcgatttc
     1561 ccccacgcca tcgagcagtt cgagcaagcc aaccaaatca tccgcgcccc tacccatacg
     1621 ctgtacatcg ctcgcgccta cgcaaaactc ggccaatggc tgaaagccag ggaactgtat
     1681 tccgagattg tacaggaacc ccttgctgcg gaggctcccc ctgctttcgt gcaagcccgc
     1741 aaagacgcgg aaaatgagtt ggaaacgctg gaagcgctca tccctcgtat caccatcgaa
     1801 atctcggggc atgccgacga aaccgtccag gtgacgatga atgacaaacc gatcgccgcc
     1861 gaagcgattg gtgttgccca acgggtcgag cctggcacct acctttttcg cgcaacctca
     1921 ggtcaattcc gggctcggtc cgtgaagatc acgatcgcgg aaaaagaacg taagaccgtc
     1981 aatctcgaac tcgaagccga acccccacca tcgccttcgc ccaccgaacc cgtcgccccc
     2041 gtgtcgacat cggcgaacca aacgactgtc tatccgtatc tgggctacgg cgctctggga
     2101 atcggagtcg cgggcatcgc cttggggttt tacttcaaca aacaacacgc ggaccgtctt
     2161 gaagagggag attccctctt cgataaatgc aatccgcgcg tttgtaccgt agaagagcgc
     2221 aatcgcatca gcgatatcga cgacgaggca tcctcgaaca aacagatgtc caccatttca
     2281 tttgtcgccg gtggggtagc gatcgtcggc ggtgccgcgc ttttgctttt cgccccttcg
     2341 caaagcggat cctcggatac atcctcgtcg gttcgaccct ggctcggatt gaattctgcc
     2401 ggtatttcgg gatcattttg atccttgctc atcgctgact tgcccggctc cgctcgagaa
     2461 gcgtacaaaa cacttgctcg acatggccga ccagccttca cgcccgtcat gacattgaat
     2521 caggagccaa cgatgaatcg gttctatcgg tatgcttccc attccgccgc tttcatgagc
     2581 ctcgctgctg ccctaggtgc ttgcaacccc tccaaaccct ctcccagcca aaccgtgacc
     2641 tcggcatctt cggtcccgtc cggatcggtg ggcgcaaccc atgcaaaaca ggaagcgaat
     2701 gccttgttga acacctatct tctcgaactg gcgaaactcg aaaaggcaca agccttggcg
     2761 tattggaccg cggccaactc agggaaaaaa gaggacttcg acgcagccgc gcgggccgag
     2821 ttggaactca aaaaattgca ctcgagtgcc gaccgttacc ggcagatcgt ctcg
//
LOCUS       DAOSOR010000678         2872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_34779_length_2872_cov_4.712460, whole genome shotgun sequence.
ACCESSION   DAOSOR010000678 DAOSOR010000000
VERSION     DAOSOR010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34779_length_2872_cov_4.712460"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PK710_23520"
     CDS             complement(<1..436)
                     /locus_tag="PK710_23520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12769.1"
                     /translation="MKHFRFTWLALAWALSSMGMLGCGSSDDTDATGADASWDSTGVS
                     DAYGADDSVPEAGADVGKEASVDVAQDGPWETSEDVIEDTQEIDVQSDVQEETSCTLV
                     RPYSVSDPECNECAQTACCEEVNACLNVDFANGRGTSARLRCN"
     gene            complement(493..2337)
                     /locus_tag="PK710_23525"
     CDS             complement(493..2337)
                     /locus_tag="PK710_23525"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOT12770.1"
                     /translation="MIGSTDGSIRLHDGVAGLYLSCCSPEPCLSPVVSRKACVGPSYR
                     VVDGFSAFVRKSRGTESEPMIRCIALTTFLGLLVVGCATSEPSEDGYAGGAGADGRAG
                     AAGSNVGGGKPKSDGGTDVEDPEEEETTCESGGSLTHPIKQEPLAAIVTSDALAPSFA
                     SYATFHTLLGMPTIVATTSEICAGQCNDADPKKDTASHIKKWLRKQLSVQYVILGGDI
                     EHVPSRKVHDRYQNPLMSDYTFEADFHTDYYFADYSKWDTNGDGVYAQDGTDFPEYRP
                     DVAVSRITASTPSEAERYYEKVRRYYTAYNLSHVAKVLMLSNVATQFMNVDIDGAWYF
                     ESEGKTLSLLPQGSEVRRLYSTGMAGAEKNSVAKQIDAIESGANLIVHSGHGTVKHLT
                     AEYSVHEDMSGEMAHDLKNDTYPIFLSSACEAATFSADDSAGEMLMDAPQGGAIAYLG
                     NSVTGLGLAGGMQMIDELLRYVRDTDHPLLADAIRMAHENMPKKDTFQVPGLNVKIPV
                     VDQASYEWTQKSVTVFGDILIPVWKGWVEPAASVTASSRPTCEGTWIDLVFDPSVDGK
                     ARVIIEQAVLEVPVVKGKARLHAAGSPSSLTVGFAVPGRLATFEKLSL"
     gene            2362..>2872
                     /locus_tag="PK710_23530"
     CDS             2362..>2872
                     /locus_tag="PK710_23530"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOT12771.1"
                     /translation="MSTPALTVWEAFAYGIIQGVTEFLPVSSSGHLRLAHQFGLGALP
                     QELELPFDVLLHAATLIAIGVAFRREIAAALAWRPTFFACVFISIVPAGLAGLLGKKA
                     VQAVGESWWLLGVCYVFTAVLLTVSERISARRALADSDRPVHEMLGDVTPKQALWVGL
                     LQVLALLPGV"
BASE COUNT          586 a          940 c          715 g          631 t
ORIGIN      
        1 gattacaacg taatctggcg cttgttcctc tcccgtttgc gaaatctacg ttcaaacaag
       61 cgttcacctc ttcacaacaa gctgtttgcg cgcattcgtt gcactccgga tcgctcaccg
      121 aatagggtct caccagcgta caagacgttt cctcttgcac gtcgctttgt acgtcgatct
      181 cctgcgtgtc ctcgatcacg tcttcggacg tttcccaggg cccgtcttgt gccacgtcca
      241 ccgaggcctc tttgccgacg tccgcaccgg cctccggcac ggagtcgtcc gccccatacg
      301 catccgacac gcctgtcgag tcccacgagg catccgcccc cgtcgcgtca gtatcgtcgc
      361 tgctcccgca tcccagcatc cccatcgaag aaagcgccca agccaaagcc agccatgtga
      421 atcggaagtg tttcatcacg tcatcttatc tcataggtcc aatacatcca acgcggtcgg
      481 tcaccggaac ccttacagag aaagcttctc gaaggttgca agacgtcccg gaaccgcaaa
      541 ccccaccgtc aacgaagagg ggctacccgc tgcatgaagc cgggcttttc ctttcactac
      601 cggcacctcg agcacagctt gctcgatgat cacacgcgct ttgccgtcca cggacggatc
      661 gaaaacaagg tcgatccacg tgccttcaca ggttggacga gacgaagcgg tcaccgatgc
      721 cgcgggctcc acccaacctt tccacaccgg aatcaggata tcgccaaaga ccgtcacact
      781 cttttgcgtc cattcgtaac tggcctggtc cacgacgggg attttcacat tcaagcccgg
      841 gacctgaaat gtatcttttt tgggcatgtt ttcgtgggcc atacggatag cgtcggccag
      901 caacggatgg tcggtatccc gcacatagcg cagcagttca tcgatcatct gcatcccgcc
      961 cgcgagccca agccccgtga ccgaattgcc cagataagca atcgcaccgc cctgcggagc
     1021 gtccatcaac atctcccccg cggaatcgtc cgccgaaaaa gtggctgctt cacacgcgga
     1081 agatagaaag atggggtacg tgtcgttttt caagtcatgc gccatctcgc cgctcatgtc
     1141 ttcgtggacc gagtactccg ccgtgagatg tttcaccgtg ccatgcccac tgtgaacgat
     1201 cagattggcg ccgctttcga tcgcatcaat ctgcttggct accgagtttt tctccgctcc
     1261 cgccattccc gtggagtaca gcctcctaac ctcgctgcct tggggaagaa gcgataacgt
     1321 cttgccttcc gattcgaaat accacgcgcc atcgatgtcc acgttcatga attgcgtcgc
     1381 cacattggac aacatcaaca ctttcgccac gtgagaaagg ttgtaagccg tataataacg
     1441 acgcaccttc tcgtagtatc gttcggcttc cgaaggcgtc gaagcggtta tcctcgagac
     1501 cgccacatcc ggacggtact ccggaaaatc ggtgccatct tgagcatata cgccatcccc
     1561 gttggtatcc catttcgaat agtccgcgaa gtaatagtcc gtatgaaagt ccgcctcgaa
     1621 cgtgtagtcg ctcatcagcg gattctgata acggtcatgc accttgcgcg atggcacgtg
     1681 ctcgatgtct cccccaagga tgacatactg cacggaaagc tgttttctta gccatttttt
     1741 gatatgcgat gccgtatctt tcttgggatc ggcatcgttg cattgccccg cacaaatctc
     1801 cgacgtcgtc gccacgatcg tgggcatgcc gagcaacgtg tgaaaggtcg catacgaggc
     1861 aaacgatggg gcaagagcgt ccgacgtcac aatggctgca agcggttcct gcttgatggg
     1921 atgggtcaac gacccaccgg actcacacgt cgtttcctct tcctccggat cctcgacatc
     1981 cgtgccccca tcgctcttag gcttgcctcc ccccacgttc gatcccgccg ctcccgctcg
     2041 accatccgcc cctgcccccc ccgcgtaacc gtcctccgaa ggttccgaag tggcacaccc
     2101 aacaaccaga aggccaagga aggtcgtcaa agcaatacat cgaatcatgg gttcgctttc
     2161 tgtgccgcgc gacttccgaa cgaaagcgga aaacccatcg acgactcgat acgatgggcc
     2221 tacacacgct tttctcgaga cgactggcga caagcacggt tcaggactac agcaagatag
     2281 atatagtccg gcaacgccat cgtgcaagcg tatcgaacca tccgttgacc caatcacgac
     2341 ggcttggtac cgctgtccgt catgtccacc cctgccttga cggtttggga agccttcgcg
     2401 tacggcatca tccaaggcgt gaccgagttt ctcccggtat cctcttccgg acatctccgg
     2461 ctcgcgcatc aattcggcct cggcgcacta ccgcaggaac tcgaattgcc cttcgatgtg
     2521 ctgctgcatg ccgccactct catcgccatc ggcgtggcat ttcgacgcga aatcgccgcg
     2581 gctctcgctt ggcgccccac ttttttcgct tgcgttttca tcagcatcgt tcccgcggga
     2641 ctggcaggat tgctcgggaa aaaagccgtg caagccgtcg gcgaatcctg gtggttgctc
     2701 ggcgtctgct acgtgttcac cgccgtgctg ctcaccgtga gcgagcgtat ttccgcccgg
     2761 cgcgcactgg ccgattcgga tagacccgtg catgaaatgc ttggcgacgt aaccccgaaa
     2821 caagccctgt gggtgggcct gctgcaagtg ctagccttgc tgcccggcgt ca
//
LOCUS       DAOSOR010000679         2872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_34781_length_2872_cov_4.256656, whole genome shotgun sequence.
ACCESSION   DAOSOR010000679 DAOSOR010000000
VERSION     DAOSOR010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34781_length_2872_cov_4.256656"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="PK710_23535"
     CDS             complement(<1..1232)
                     /locus_tag="PK710_23535"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT12772.1"
                     /translation="MSEKEYEPPTSATPSGVLGSIGAYQLTEVLRRTRVAEEFLARQR
                     GPGGFERVCLLKVAQRNRTDLRGAEMLGREAKVLMRLDHPCIVRFHDFFELGDRVVLV
                     LEYFSGLTLERFLELLVEHEEVLDERITWHVALALFEALAHVHGLGGEGESGSVVHRN
                     ISPSNVLLSSSGRVRVTGFSAVQDGDTLQEATALGEIVRVPSYLAPEQLRGSAASEEA
                     DAFSAALIVWELLTGQRATPEGLSEFDLLKVLSTRHPDSLRVLRPDVPALVTTALDLC
                     LMPNPQERRIRCEEVAGCISAGLPPGDGATVLRETIARMGPAVEKLAGGKAQSTPPSR
                     RPGSTPPLPSRPEQITLPDRVRISARMEAAVTAIPRAPGSRPSLPSLPAALPDLLPDD
                     SQPSIIAYEANEVAEKQVL"
     gene            complement(1500..>2872)
                     /locus_tag="PK710_23540"
     CDS             complement(1500..>2872)
                     /locus_tag="PK710_23540"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOT12773.1"
                     /translation="IIEYAVRNVAVAISWANYFRTLLEVAFGIQVPAWLATDARTAAH
                     IPGLLESAPHVLGIPIVFNLLAFLIVAVITMVLVWGIRESARFNAGMVVIKIVVLLFF
                     IGVALYFVPSDKIVQNWQPFQPRGWGGTLAGAAVVFFAYIGFDAVSTVAEETRNPSRD
                     LPIGIIASLTICTLLYVVVAAVFTGMMPFPELVQRLATEQAEPLTMALDHVAPQATWA
                     SGIVAFGSVIAHTAVLLVFQLGQPRIFFSMARDGLLPPSFARVHPRFKTPHVTTILTG
                     LVVGTVAAFASIDEMVDLTNIGTLFAFVLVCLGIPILRHTDPDRKRPFRVPFGPYLIP
                     VAGVISCLFLMVYLPPASWWRFIGWLILGLAVYCAFGYTHSHLGREAGRVQQAPIGLK
                     VAAVALLLTSLGLFAMPHGLGWLELLGAMGRGEPRAVLGIGLAFGGAMVASLALWRTR
                     IALRAR"
BASE COUNT          680 a          890 c          789 g          513 t
ORIGIN      
        1 agaacctgtt tttccgcaac ctcattggct tcataggcaa tgatgctggg ctgggaatcg
       61 tcgggcagta gatcgggcaa agccgctggc aacgaaggca ggctaggacg ggatcctggg
      121 gcgcgaggaa ttgccgtgac cgctgcttcc atccttgcgg agattcgcac tcgatctggc
      181 aaggtaatct gttcggggcg agaaggtaag gggggagtgg aacctggacg acgggatggg
      241 ggggtggatt gagctttacc ccccgccaat ttttccactg cgggtcccat tctggcgatg
      301 gtttccctca agaccgttgc gccatcgccc ggcggtaatc ccgcggaaat gcagcctgcc
      361 acctcttcgc agcggattcg acgctcttgg gggttgggca tcaaacataa gtcgagggcg
      421 gtggtgacga gggcaggcac atcggggcga agcacgcgca aggaatccgg atgtctcgtg
      481 gataaaacct tgagcaagtc gaattcggaa aggccttccg gagtagccct ttgtccggtg
      541 agcaactccc acacgataag agccgcggaa aaagcatcgg cctcctccga cgcagcgctt
      601 cccctcaatt gttcgggcgc aagatagcta ggaacccgaa cgatctcgcc aagagccgtc
      661 gcttcttgca acgtatcgcc atcctggacc gcggaaaatc cggtgacacg cacccttccg
      721 ctcgaggata gcaacacgtt ggaaggcgag atatttcgat gaacgaccga gcctgattcc
      781 ccttcccctc cgagtccatg aacgtgagcc aaggcctcga aaagcgccaa cgccacgtgc
      841 caggtgatac gttcgtcgag cacttcctcg tgttccacca atagttcgag aaatcgttcc
      901 agcgtcaatc cactgaaata ttcgaggacg agaacgaccc tatcgcccaa ttcgaaaaag
      961 tcgtggaaac gaacgatgca cggatgatcc aggcgcatga gcactttggc ttcgcgtcca
     1021 agcatctcgg ccccccgcaa gtccgtgcga ttgcgttggg ctactttgag caaacaaacc
     1081 cgttcgaacc cgcccggccc acgctggcga gccagaaact cctccgccac cctcgttcgg
     1141 cgcaggactt cggtaagttg gtacgcaccg atcgatccga gcacgcctga aggtgtggcg
     1201 cttgtcgggg gctcatactc cttttcgctc atcctagcgc tctttccaac gatgcttcgc
     1261 cgtcatggaa gccgcgaaac gaaattccat agctgaaagc gtgcgaacca tgcggccgca
     1321 tcttggtcac acacctgctg aaaaatgcaa gccctcttgt cgcctgcatt cgattatctc
     1381 tttgccatcg ggcatgcgct ttcaccgttg cccccctgta tcccataagt ccttgaaaaa
     1441 cggtattatt tctgcagttc ctccgtccgc tccccgcgga gtcattcgcg aatccaccgc
     1501 taccgtgctc gtaaggcaat ccgcgtgcgc cacaaggcca gcgaagccac catcgccccc
     1561 ccaaacgcca ggccaatacc caacacggcc cgcggctccc ctcgtcccat cgcgccgagc
     1621 aattcgagcc agccgagacc atggggcatc gcaaatagcc ccaacgacgt cagaagcagc
     1681 gccaccgccg cgaccttcaa ccctatcgga gcctgctgca cccttcccgc ttctcgtccg
     1741 agatggctat gcgtatagcc gaacgcacaa tacaccgcca agccgagaat caaccagcca
     1801 atgaagcgcc accaggaggc aggtggcagg taaaccatca aaaataaaca agaaattaca
     1861 ccggcgaccg ggatcagata gggcccgaaa ggaacgcgga aaggacgctt tcggtccgga
     1921 tcggtatgac gcaaaatcgg aatccccaga catacgagca cgaacgcgaa tagcgtaccg
     1981 atattcgtca gatccaccat ctcgtcgata ctcgcgaagg ccgctaccgt tcccaccacc
     2041 agccccgtca gaatcgtcgt tacatgaggg gttttgaaac ggggatgaac ccgcgcgaac
     2101 gatgggggca acagaccatc cctggccatg gaaaagaaga ttcgcggctg cccgagttgg
     2161 aaaaccaaca gcactgccgt atgggcgatg acgcttccga acgcgacgat gcccgaggcc
     2221 caggtggcct ggggagccac gtgatcgagc gccatggtga gcggctcggc ttgctcggtt
     2281 gccagccgtt gcacaagctc cggaaacggc atcatcccgg taaatacggc cgcgacaacc
     2341 acgtacaaaa gcgtgcaaat ggtcagcgaa gcgatgatgc cgatgggcaa atcccggctt
     2401 ggattgcgcg tttcttccgc gaccgtcgag accgcatcga aaccgatata ggcaaaaaat
     2461 acgacggccg ccccggccag ggttcctccc catcccctgg gctgaaacgg ttgccagttc
     2521 tgaacgatct tgtccgaagg cacgaaatac aaggcaaccc cgatgaaaaa caacaacacc
     2581 acgatcttga tcacgaccat gcccgcgttg aatcgcgcgg attcccggat gccccagacg
     2641 agcaccatgg tgatgacggc cacgatgaga aaagcgagaa gattgaatac gatggggatg
     2701 cccaaaacgt ggggagcgct ttcgaggagc cccgggatgt gcgcggccgt cctcgcatcc
     2761 gtcgccagcc aggcgggaac ctgaatgccg aaggccactt cgagaagagt tcgaaagtaa
     2821 ttcgcccagc ttatcgccac cgccacattg cgcactgcat actcgatgat ca
//
LOCUS       DAOSOR010000680         2851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_35128_length_2851_cov_3.227110, whole genome shotgun sequence.
ACCESSION   DAOSOR010000680 DAOSOR010000000
VERSION     DAOSOR010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35128_length_2851_cov_3.227110"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1400)
                     /locus_tag="PK710_23545"
     CDS             complement(<1..1400)
                     /locus_tag="PK710_23545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12774.1"
                     /translation="MNASVALNQAKSALSVGDVQQVLDLFCRIVATESDLFPWILLLI
                     EAGHLHVASEHADKLRFDEQARIRELLQEESGDWEGQGDPGDAYGIDDEVLRPRAEVQ
                     EELISLFLRFYGGRRDIYAKAWYDERRKRAGYHPVQQPLTESVVRAHLQGSMTIGQYL
                     LYPDGACAYGVIDLDVSASVLQTLRVARGEEASALMHPPLGRYVLTLLDAGERLGLPL
                     FAEDSGGRGVHLWLHMEPRRAASAVRAMLSQVIVAAGPIPPEVGVEVFPKQDRLGPRG
                     LSSLVKLPLGVHPATLRRCALLDRKLKVYSSALEGLRALRLVPMDAVDAVVGRRVVPL
                     PAPEITPVRAVPRLDTRPTPRSLAETLRQIEVGVPEKEAVDAVLRGCPVLAGLTKKAF
                     ENHSLLADEARALLYTIGLISPGPGVIDEVLASAKIPRRELDRLRRGLPNPAGCKKLS
                     RLAPEGTCSCFEKNEVL"
     gene            complement(1397..2629)
                     /locus_tag="PK710_23550"
     CDS             complement(1397..2629)
                     /locus_tag="PK710_23550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12775.1"
                     /translation="MAEARSHGISDPHATLIEAEALMRKQAWEEVAALGLRLPTPPDE
                     SWLAVMDVVAFALGQLRQWTVAIALLEHAYGLEATWRRASSLAYLYYDGALASQAPRG
                     PKPDMSKEALRKGFRRWIAEALRHDPDSIKDIYRLGIFEAQVEACHDKPALRAFLRVI
                     DLFHALSEEEKARRGDYRKVCSKALYAGARSALRLKQLGLARKLSFACIREDKEANHV
                     EPVHKLQMAARVCMATGEFDHAERAARLALDAPGPPRRDYLFGLLSDISLLRSDPEAA
                     CSWIEKHVPEHRRASYLWRKLGDGRAARGQLKEACQAWESALRQDRCGRHLTLVRLGR
                     AYLDLGALGKAEDAFRKAAHFRVSRFMKEDREALEGLEKVLALRGKKEELPRIAERLA
                     KCGGQGQPASDDDELGVA"
BASE COUNT          645 a          947 c          714 g          545 t
ORIGIN      
        1 agcacctcat tcttctcgaa acacgagcag gtgccctcgg gagccagacg agacagtttt
       61 ttgcacccag cgggattggg caagccccga cgaaggcggt cgagttcacg acgagggatt
      121 ttcgcactgg ccagcacttc atcgatgact cccggtcccg ggctgatcaa tccaatggtg
      181 tagagcaagg cacgcgcctc atccgccaat aagctatggt tttcaaaagc ttttttcgtt
      241 aatccggcca gcacgggaca gcctcgaagc accgcgtcga ccgcttcttt ttccggcacc
      301 cccacttcga tttgccgcaa cgtttccgca agagaacgag gtgtcggacg cgtatcgagg
      361 cgcggtacgg cccgcacggg tgtgatttca ggcgctggca ggggcactac cctgcgcccc
      421 accaccgcgt ccaccgcatc catgggcacc aggcgcaacg cgcgcaatcc ctccaaggcg
      481 ctcgaatata ccttcagttt cctatccaat agcgcgcatc gacgaagcgt ggccggatga
      541 acccccagag gcaacttgac caaactcgat agtccccgcg gacccaagcg atcttgcttg
      601 ggaaaaactt ccactcccac ctccgggggg atcggtccgg cagccacgat cacttgggaa
      661 agcatggctc gaaccgcgga agcagcacgg cgcggttcca tgtgcagcca aagatgaacc
      721 cctcgcccac cactgtcctc cgcaaacaag ggtaacccca atcgctcccc cgcatccagg
      781 agcgtcaaaa catagcgccc cagcggcgga tgcatgagcg ccgacgcctc ctctccccgc
      841 gccacccgaa gcgtttgcag cacggacgcc gaaacgtcga ggtcgatgac cccataagca
      901 caggccccat ccggataaag caggtactgc cctatcgtca tcgaaccttg cagatgcgcc
      961 cgaaccacac tctccgtcaa aggctgctgc acaggatggt atcccgcgcg cttgcgacgc
     1021 tcatcgtacc aggctttggc ataaatatct cgcctgccac cgtaaaaacg aaggaaaagc
     1081 gagatcagtt cctcttgcac ctcggcacga ggacgcaata cctcatcgtc gataccatag
     1141 gcatccccgg ggtcgccttg gccttcccaa tcacctgatt cctcttgcaa aagctcacga
     1201 atgcgagcct gctcatcgaa cctcaacttg tccgcatgtt cggaggcaac gtgaaggtga
     1261 ccggcttcga tcagcaacaa gatccatgga aataagtcgg attccgtggc aacgatgcgg
     1321 cagaacaagt cgagaacctg ctggacatcc ccaacactca acgcgctttt ggcttggttc
     1381 aaggcaacgg aagcgttcat gccaccccca attcatcgtc gtccgacgcg ggttgtcctt
     1441 gtcccccgca cttggccaat cgttcggcaa tgcgcggtaa ttcttccttt tttcctcgaa
     1501 gggcgagcac tttttccaat ccttcgagag cctcccggtc ctccttcatg aaacggctga
     1561 ctctgaaatg tgcggccttt cgaaaagcat cctcggcttt gcccaaagcg ccgaggtcga
     1621 gataggcacg gcccagacgc accaacgtta gatgccttcc gcagcggtcc tgacgcaaag
     1681 cggattccca agcctgacag gcttctttca gttgccctct ggccgcccgt ccatcgccta
     1741 attttctcca taaatacgat gccctacgat gttccggcac atgcttctcg atccaggagc
     1801 aggccgcctc gggatcggag cggagcaggg aaatatcact caaaagcccg aaaagataat
     1861 cacgcctcgg tggcccaggg gcatccaggg caagacgagc cgcccgctcc gcatgatcga
     1921 attcgcccgt cgccatgcat acccgcgcgg ccatttgcaa cttatgcacc ggctccacgt
     1981 ggttcgcttc cttatcctcc cgaatgcagg cgaatgatag tttacgagcc aatcccagtt
     2041 gtttcagccg caacgccgaa cgagcccccg cgtaaagcgc tttggagcaa actttcctgt
     2101 aatcccctcg acgcgctttt tcttcttccg aaagagcatg aaaaaggtcg atcacacgta
     2161 aaaacgcccg caacgccggt ttgtcgtgac aggcctccac ctgcgcttcg aaaatcccaa
     2221 gccgatagat gtccttgatg gagtccggat catgccgtaa tgcttccgct atccaacgtc
     2281 gaaagccctt tcgtaacgcc tccttgctca tgtccggctt cgggccgcgc ggcgcttgcg
     2341 aagccaacgc cccatcgtag tacaaatagg ccaaactcga cgcccttcgc cacgtggcct
     2401 ccaacccgta ggcatgctcg agcaacgcaa tggcaaccgt ccactgacga agctgtccca
     2461 gggcaaaagc caccacatcc atgactgcaa gccaggactc atccgggggg gttggcaacc
     2521 gaagccccag tgcggccacc tcttcccagg cttgcttgcg catcagcgct tcggcctcga
     2581 tcaaagtagc atggggatca gaaataccgt gggatcgcgc ttcggccatc ggcaactcct
     2641 tttctccttt caagacgcac gggagcttgg agatctgaca gccacggagc aaataccttt
     2701 tccctggaga aaaacgggcc tgcaccccgt cccccacctg attcaaaagc cacgttgccc
     2761 tggcaacgcc ccgtcgtttt cagcggagcc tacgaaaatc agcgaaccga acgtcgaaaa
     2821 cgaaaattcg gcgcaagata atggctcgca t
//
LOCUS       DAOSOR010000681         2846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_35193_length_2846_cov_3.745969, whole genome shotgun sequence.
ACCESSION   DAOSOR010000681 DAOSOR010000000
VERSION     DAOSOR010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35193_length_2846_cov_3.745969"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PK710_23555"
     CDS             <1..531
                     /locus_tag="PK710_23555"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT12776.1"
                     /translation="LHVVINHLRYLGASGSVRRIPKHRFSVRVMEHQGAEIREFFDQD
                     TITIGRAPGNDLMIPDYSVSRQHAQVHFADGSFTVHDLGSKLGTVVSGKRVSDTLSFQ
                     PGERLELGDFELVFEGPIDASYRTQYSSLMAESPTGYPPPVMEGMELASGLLEAPALS
                     QEEVRELNDLDVIDEE"
     assembly_gap    581..680
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(804..2813)
                     /locus_tag="PK710_23560"
     CDS             complement(804..2813)
                     /locus_tag="PK710_23560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12777.1"
                     /translation="MAVVLGSGPPVEQKGKMYVRSANQGTWTPFNLANQTLYEKKKKD
                     GSHSVLLKTTDSSKLVLDGGFEVNFKVKWNKVHFFTKFYGGYDFNLGLDGTLDFEGSD
                     SYTKKLFPTGNDDWKTLAEFSILGIPVHIDFRLEAVAKVQASATGSFMSEYSNRRSYG
                     FWFSMTNGDWDSGTIHTHDSEWKQPPTWTLEGKATFEAGLKPILALGLGGKYAGVGAQ
                     AHAGFTAFPRLKLDAELKANSHAASTLDWQLDACLDLGVHASAKADFWGYEKSKTWEK
                     VLWEGCMKLDDGSKCFPYCEGKNCGPDGCDGVCGSLQGQCEPGWVCNDGVCQCVPDCT
                     NRVCGSDGCGGTCPSGCDQYGEPYECIEGTSKSVCACVPDCSGKECGDNGCGGQCGTC
                     EIGKRCNASHQCEPIPDACVPECGGRQCGRDPVCWLPCPVGNPGSCHEPGDTCSHHGF
                     CCRIAEQFHGTGSPGTYSGALSCSLGSTPGSEAALVTPTSVSDIPSTPFALKLKAGGK
                     DLVTCTEKFRCEAITDLPFRRAETMLWVDEPAAGQAMARAGFGFGPNLDWAVVMDGVA
                     KKAYITHGNHQTDLGAIEVKRWYKILLRAKDDFSQMYFEVPGVGGTWVPAGQAMWEAI
                     RFQTTVTNPPQTWCPLGIGTVGATTAGGLELIPYTAYFDDVHACR"
BASE COUNT          622 a          787 c          738 g          599 t
ORIGIN      
        1 cttcacgtag tcatcaacca tttgcgttac ttgggtgcga gcggcagtgt acgtcgcatt
       61 ccgaaacatc gcttctccgt acgggtcatg gagcaccagg gagcggaaat acgagagttc
      121 ttcgatcaag atacgattac gatcggccga gcccccggca acgacctcat gattcccgat
      181 tacagcgtat cccgacaaca cgcgcaggtt cactttgccg acggctcttt cacggtgcat
      241 gaccttggaa gcaagctggg tacggtggta tcgggaaagc gtgtcagcga cacactatcg
      301 tttcaacccg gagaacggtt ggaattgggc gatttcgagc tggttttcga gggccccatc
      361 gatgccagtt atcgcaccca atactcgtca ctcatggctg aatcgccaac aggttatcct
      421 ccaccggtga tggaaggcat ggagttagcg tcgggattgc tcgaagcgcc cgcgttatcc
      481 caggaggaag ttcgcgaatt gaacgatttg gacgtgatcg acgaagaatg aagccacgac
      541 ttggactgcc caagagcttg cccgaatacg gcgatcttgt nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn cgtaggcaag gcatcgtagg caaggcatcg taggcaaggc
      721 atcgtaggca aggcatcgta ggcaaggcag tattgggtcc ccggcgtcgt gcggtaaggg
      781 gttcaaacga tacctatgat gtatcaacga caggcatgaa cgtcatcgaa gtaggcggta
      841 tagggaatga gttcgagacc tccagcggtt gtggctccga cggttccgat gcccagcgga
      901 caccaggttt gaggcggatt agtaaccgta gtctggaaac gaatcgcctc ccacatcgct
      961 tgtccggcgg gaacccaggt gcctccaacc ccaggcacct cgaagtacat ttgcgaaaag
     1021 tcatccttgg cccgaagaag gattttgtac catcgcttga cctcgatggc tccaaggtca
     1081 gtctggtggt tgccatgggt gatataggct tttttggcca caccatccat gaccacagcc
     1141 caatcgaggt tgggtccaaa accgaaaccg gctctcgcca tcgcttgccc cgctgcgggc
     1201 tcatccaccc aaagcatcgt ttccgcacgc cggaaaggca gatcggtgat ggcttcacaa
     1261 cggaattttt cggtgcaggt cacgagatcc ttgccgcctg ctttgagttt caacgcgaaa
     1321 ggagtgctgg gaatatcact gacagaggtt ggggtgacaa gcgccgcctc ggagccaggg
     1381 gttgagccga gcgagcatga aagggcgccg gaataggttc ccggcgagcc agtgccgtgg
     1441 aattgttcgg ctatcctaca gcagaaaccg tgatgggaac aggtgtcacc gggttcatgg
     1501 caggatccgg ggttgcccac agggcaagga agccaacaca cgggatctcg cccgcattgg
     1561 cgtcccccgc attccggaac gcaagcgtcg ggaatgggct cacattgatg cgaggcgttg
     1621 caccgcttgc cgatctcgca ggtgccgcac tggcctccgc aaccgttgtc cccacattct
     1681 ttgcccgagc aatccggaac gcaagcgcat acgctcttcg acgtcccctc gatgcattcg
     1741 tagggctctc cgtattgatc gcatcctgac ggacatgtcc cgccgcagcc atcactaccg
     1801 cacactctat tggtgcaatc tgggacacat tggcaaactc catcgttgca cacccacccc
     1861 ggttcgcact ggccctgcaa cgacccgcaa acaccgtcac aaccgtcggg gccgcaattc
     1921 ttcccctcac aatacggaaa gcacttgctg ccatcgtcga gcttcatgca cccttcccaa
     1981 agcacttttt cccaggtctt cgacttctcg tatccccaaa aatccgcctt ggcgctcgca
     2041 tgcacaccaa ggtcgagaca cgcatcgagt tgccaatcga gggtactggc cgcatggctg
     2101 ttggctttca gttcggcatc cagcttcaag cgaggaaatg cggtgaatcc agcatgagcc
     2161 tgggcgccca cccccgcgta cttgcccccc aacccaagcg caagaatcgg cttcaatccc
     2221 gcctcgaaag tcgccttgcc ctccaacgtc caggtcggag gctgcttcca ttcgctatcg
     2281 tgtgtgtgaa tcgttccgga atcccagtct ccgttggtca tggagaacca aaaaccatag
     2341 cttcgacgat tcgagtattc ggacatgaac gagccggtgg ctgacgcttg cactttggcg
     2401 acagcttcga gtctgaaatc gatgtggacg ggaattccca aaatggaaaa ctccgccagg
     2461 gtcttccaat cgtcgttgcc cgtcgggaac agctttttgg tgtaggaatc ggaaccttcg
     2521 aaatccaacg tgccatcgag accaaggttg aagtcatatc caccgtaaaa tttcgtgaaa
     2581 aagtgaactt tattccattt aaccttgaag ttgacttcga atccgccatc caagacaagc
     2641 ttgctgctgt cggtggtttt cagaagaacg gaatgactcc catctttctt cttcttttcg
     2701 taaagcgttt ggttggccag gttgaaagga gtccaagtcc cctggttggc agatcgaaca
     2761 tacatcttgc ctttttgttc gacaggcggc ccagagccta gtactacagc cacgaagcac
     2821 gttccgctta tttatggaaa taaatt
//
LOCUS       DAOSOR010000682         2840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_35289_length_2840_cov_5.265709, whole genome shotgun sequence.
ACCESSION   DAOSOR010000682 DAOSOR010000000
VERSION     DAOSOR010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35289_length_2840_cov_5.265709"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1553
                     /locus_tag="PK710_23565"
     CDS             <1..1553
                     /locus_tag="PK710_23565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12778.1"
                     /translation="GACSSTPVVDVVLQDPDGRRAYAKYAQLVIFDNGCPDRTKLANG
                     DVSGYRWMQSVEVDGNFNEIGTLDKAPYGFAAILRDDYCGVIGFGCTPVDLSHHRHIA
                     IAVNEEVSPPRGSCDTDQVCANSVCIAGNPTEEDADTEEDIGPLNCEFDIIASGNFDL
                     PATASTVYSGPVVVATSNGFVIMYREAEPSGLHPVGVRLKVTDQGNVTRNNINLPACA
                     QNNRDDGIAAAWNGSLGAGLMAVALPSCGPENAQLHVSNFDRDGKTLADHQYKNVPAP
                     LHIHPINAMAPAPASKNFLLATMQGPVPLLFVFNGITVQGDPPPSEIHKGNGKATFTQ
                     ISSSESIRSLLTDSDLDDGKILVSVVDISTGSESMTDFEHSPITSLVSWSERTVVAQP
                     SGDSLVWKAITKSGSSIKSGTLDGGPYTSVGSAQLHDYLLLAGAKKGAITMFRIDDAN
                     GTFSGSPFQSTLSSSFGLSAIEGFQGERIGIAAARKRVVVAWITSTVPMANTSTAPGG
                     FAVLACRG"
     gene            1546..2823
                     /locus_tag="PK710_23570"
     CDS             1546..2823
                     /locus_tag="PK710_23570"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOT12779.1"
                     /translation="MANRRRARVFRARIKALAKGGNGVASVLSGQGQNSILVPGTLPG
                     ELVEIEQISKTKASLLRILEPSAHRIAPPCTYANSCGGCDWMHLDLAIQRRTRPILVH
                     DELHRVGIPTPDIGSHHAEMNLAYRARARLAVVGTSHGIIVGFRASKSRRIVPVDTCL
                     VLEPVLDIARAELASWLDGSEGMGEVTLMLGRNHLPAIYVTWKGTLNANVFASAQDNV
                     EQRRWAGVSIWLDGATVPATMGDPRGVALGVDGHDLFIPPGGFMQAHNQMNPTLVGHV
                     QSIAETLGHPILELFAGSGNFSIALARQTPDLETVEQQDAAVAAARSNLETRGLRARL
                     RVDDAEKTIVRSAVRTVVLDPPRTGAKRAVQTIAASRARDVVMVSCDPATLARDSAIL
                     VHQGRFELHRIDVFEMFPHTSHVETVACFRRKR"
BASE COUNT          618 a          812 c          795 g          615 t
ORIGIN      
        1 tcggagcttg ttcctccacg cctgtcgtcg acgttgtgtt gcaggatcct gacggtcgac
       61 gtgcttacgc gaagtacgct caactcgtca ttttcgacaa tggttgtccc gacagaacga
      121 agttggcgaa cggtgacgta tctggatatc ggtggatgca gtctgtggaa gtggacggca
      181 acttcaatga gatcgggacc ctcgacaagg ctccctatgg gtttgccgcc atcttgcgcg
      241 acgattactg tggcgtgatt gggtttggtt gcacaccggt ggatctttcg catcatcgtc
      301 acatcgcgat cgcggtcaac gaggaagtct cgccaccacg aggcagttgc gacacagatc
      361 aggtctgtgc aaacagtgtt tgcatcgccg ggaacccgac ggaagaagat gcggatacgg
      421 aagaggatat cggtccgctc aattgtgaat ttgacatcat cgcatcgggc aatttcgatc
      481 tgcctgcgac cgccagcacg gtgtattcgg ggcccgtcgt cgttgccacg tccaacggtt
      541 ttgtcatcat gtatcgagaa gcggaacctt cgggtctgca ccctgtcggt gttcgcttga
      601 aagtcacgga ccaaggaaat gtgactcgca acaacatcaa tttgcccgca tgtgcgcaga
      661 acaatcgcga tgatggcatt gctgcggcgt ggaacggaag tctgggggct ggtctgatgg
      721 cggtggctct tccttcttgc ggtcccgaaa acgcacagct tcacgtgtcc aacttcgatc
      781 gcgatggcaa gacgctggcc gaccatcaat acaaaaatgt gcccgctcct ttgcacattc
      841 atcccatcaa tgcgatggcg cctgcaccag cgagcaagaa tttcctattg gccaccatgc
      901 aaggccccgt gccgctgcta ttcgtattca atggcattac cgtgcagggc gatccccccc
      961 cttctgaaat acacaaagga aacggcaaag caacgttcac gcaaatctct tccagcgaaa
     1021 gcatccggtc gttgcttacg gactcggatc tcgacgatgg caaaatcctc gtaagcgtgg
     1081 tcgacatcag taccggaagc gaatcgatga ccgatttcga gcattcaccg atcacgagtc
     1141 tggtgtcttg gagcgaaaga accgtagtcg cacaacccag cggcgactcg ctggtttgga
     1201 aagcgatcac caagtccgga tcgtccatca aaagtggcac tctcgacgga ggtccctata
     1261 cctcggtagg ctcggcccaa ttgcacgact acttgctgct cgcaggagcg aaaaaaggcg
     1321 ccatcaccat gtttcgtatc gacgatgcga acggtacgtt ctccggttct cccttccaaa
     1381 gcacgctttc atcgtccttt ggcttgtccg ccatcgaggg ctttcaagga gaaaggatcg
     1441 gcatcgcagc cgcacgaaaa cgtgtcgtgg tcgcttggat aaccagtacc gtaccgatgg
     1501 ccaacaccag caccgcgccg ggagggttcg ccgtgcttgc ctgccgtggc taatcgtcga
     1561 cgcgcccgtg tgtttcgtgc gcgcatcaag gcgttggcca aaggagggaa cggagttgct
     1621 tccgttctat ccggacaagg acaaaatagc atcctcgttc ccggcacgct gccaggcgaa
     1681 ctcgtcgaaa tagaacaaat tagtaagacc aaagcctctc tcctccgcat cctagaaccg
     1741 tccgctcatc gaatcgctcc accatgtact tatgcgaata gttgtggagg atgtgattgg
     1801 atgcatctcg atctggccat ccaacggcgt acacggccca tcctggtcca cgacgaactg
     1861 catcgggttg gtatccccac accggatatc ggttctcatc acgcggaaat gaacctcgcc
     1921 taccgtgcgc gcgcgcgctt ggccgtcgta ggcacaagcc atggcatcat cgtgggattt
     1981 cgcgcatcca agagccgacg catcgtcccc gtcgatacgt gtctggtgtt ggaacccgtg
     2041 ctcgacatcg ctcgcgctga gcttgcctct tggctcgacg gcagcgaggg catgggcgaa
     2101 gtgacattga tgcttggacg caatcacttg cccgccatct atgtcacttg gaaaggcacg
     2161 ttgaatgcca acgtattcgc aagcgcacag gacaatgtcg aacaacggcg ttgggcggga
     2221 gtgagcatct ggctggacgg tgcgacggtt ccggccacga tgggggaccc acgcggcgtg
     2281 gcgctcggtg tggatggtca cgacctattc attcccccag gcggattcat gcaagcccat
     2341 aaccaaatga atccaacgtt ggttgggcac gtgcaaagca tcgccgagac gcttggccat
     2401 cccattctcg aattattcgc tggctccggc aatttttcca tcgcccttgc tcgtcaaacc
     2461 ccggatttgg aaaccgtaga acagcaagat gcagccgttg cagccgcacg ttccaatctc
     2521 gagacacgag gtctgcgtgc tcggctgcgc gtcgatgacg ctgaaaagac gatcgtacgg
     2581 tctgccgttc gtacggttgt gctcgacccg ccgagaacgg gcgcgaaacg agccgttcag
     2641 accattgcag caagtcgcgc gcgagacgtg gtgatggtgt catgcgatcc ggcaaccttg
     2701 gcacgagatt ccgcgatcct ggttcaccaa ggacgcttcg agcttcatcg tatcgatgta
     2761 ttcgagatgt ttcctcacac gagccatgtg gaaaccgtgg cgtgttttcg gagaaaacga
     2821 tgattcaatc cgtggacgag
//
LOCUS       DAOSOR010000683         2828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_35477_length_2828_cov_4.474937, whole genome shotgun sequence.
ACCESSION   DAOSOR010000683 DAOSOR010000000
VERSION     DAOSOR010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35477_length_2828_cov_4.474937"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PK710_23575"
     CDS             complement(<1..581)
                     /locus_tag="PK710_23575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12780.1"
                     /translation="MVGKDGTKKTADGEPKAHRFGAVTHEFSTSKKTSPWPRRAIEVS
                     GVSGATLVPVGLVAVEGTLGIVVAVVGGLMALAAYVLSASKRHRGLTTKVVLHELGIV
                     CSQGRQSRELMWNEVVDITYKTVSIPGAKATIALVFETVHPPPFLLMVGGPYGDERAT
                     ALLIEKLGKIWLDVWCRRAKVLAELDGIQVGKA"
     gene            complement(581..1657)
                     /locus_tag="PK710_23580"
     CDS             complement(581..1657)
                     /locus_tag="PK710_23580"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HOT12781.1"
                     /translation="MKASLSWLRSLIHGLDATDQQIVQILSLAQWAVKKCDRSHDDTV
                     LELADTARTDVLGHIGLAREIAALFRQPFEPPVVDAPVRITHGTMRVPIEVKETTACL
                     GFGALFVDNVHVESSPDWLSHRLKTLGHRPDNNVVDITNLVMLEYGHPVEAIDFDRIE
                     GSLEVRFARSGETLQLEDKRLRLHPDDLVIVSNGGPIALAGIAFCPSLHIGANTRRIM
                     LSSVCVDLTSMMRTMQRHGLRSEHSQRPSRGGYREDLMDLLAQAGSLTTHLARGEAVT
                     GSVHIRLAPENLWYGNVRPPSEIGWQQAVQILERLGCEVVPVHGAEGSLQVRLPWFRH
                     DLHNEEDIVKELRRVNLVWTTWSR"
     gene            complement(1704..>2828)
                     /locus_tag="PK710_23585"
     CDS             complement(1704..>2828)
                     /locus_tag="PK710_23585"
                     /inference="COORDINATES: protein motif:HMM:NF027969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOT12782.1"
                     /translation="RSGLFLGVYHRTLDKLMLFDESRAGSSATQGAMVVPMNVGDLDD
                     PRLRAQIGQDGCEQLEQDIDLLNAAGDAFDPSSFLQGRLSPMFFGSAINNFGLETFLD
                     SFIQMMPPPGPRASSIGLVDPGRQEFTGFVFKVQANMNPAHRDRVAFVRICSGQFHRK
                     TKVLHVRTDREVRLANPTQFIAKERTIVEEAYAGDVIGVYDPGFFQVGDTLTGGTKLE
                     FEPIPSFAPEYFKRLVMVDPLRRKQLVRGTTQLAQEGTVQLYQPPDIRTGDMILGAVG
                     LLQLEVVKHRLASEYGVDVRLESVPFHLARWVTRTDGQPVDLSHVRQQIDAMLVVDVR
                     DRPVVLFEAEWNLNSAVKFHPEYQFAETAQGVIVRDPQND"
BASE COUNT          647 a          839 c          750 g          592 t
ORIGIN      
        1 cacgctttcc ccacctggat gccgtctagc tccgcaagga ctttggcgcg acgacaccac
       61 acgtccagcc atattttgcc gagtttttcg atcaacagag cggttgctcg ttcgtcgccg
      121 taagggcctc ccaccatgag aagaaaaggc ggcggatgaa ccgtttcgaa caccagcgca
      181 atcgttgctt tcgcgcctgg gatagaaacg gttttgtaag tgatatccac cacttcgttc
      241 cacatgagtt cgcgagattg ccttccctgc gaacatacga tccccaactc gtgcagcacg
      301 actttcgtag tgagcccccg atgtcgtttg ctggctgaca atacgtaggc cgccaaagcc
      361 atgaggcctc ctaccaccgc caccacgatg ccaagcgtac cttccaccgc caccagcccc
      421 acgggaacca acgtcgctcc actcacaccg gatacctcga tggctcgacg gggccaaggc
      481 gaggtttttt tcgacgtgct gaactcatgg gtcacggcac caaagcgatg tgcctttggc
      541 tcaccatccg ccgtcttttt ggttccatct ttgccaacca tcaccgactc caggtcgtcc
      601 aaaccagatt cacccttcgc aattccttga cgatatcctc ctcgttgtgc aggtcgtgac
      661 gaaaccaagg cagccgcacc tgcaaagaac cctcagcgcc gtgaacggga acaacctcgc
      721 atccaagacg ttccagaatc tgaaccgctt gttgccagcc gatttcggat ggcggacgaa
      781 cgttcccata ccacaaattc tccggtgcta gccgaatatg gacagaaccc gtgaccgctt
      841 cgccacgcgc cagatgggtt gttagtgacc cggcttgtgc caatagatcc atcaggtcct
      901 cgcgatatcc accacgcgat ggtcgttggg aatgctccga acggagtcca tgacgctgca
      961 tcgtgcgcat catagaagtg aggtccacgc acacactgct gagcatgata cgacgtgtgt
     1021 tcgcgccaat gtgcagcgag ggacagaaag cgatacccgc aagcgcgatc ggtccgccat
     1081 tcgagacgat cacgagatcg tcgggatgca gtcggagacg cttgtcttcg agttgtaacg
     1141 tctccccgga ccgcgcaaac cggacctcga gggaaccttc gattcgatcg aaatcgatgg
     1201 cctccacggg atgaccgtat tcgagcatga ccagattcgt gatatccacc acattgttgt
     1261 ccggtcgatg gccgagcgtt ttcaatcgat gagataacca atccggcgat gattcgacat
     1321 gcacattgtc gacgaacaaa gccccgaatc cgagacaagc cgtcgtttcc ttgacctcga
     1381 tcggcacccg catcgtaccg tgagttatgc gaacgggcgc atcgacgacc ggtggttcga
     1441 aaggttgacg aaacaatgcc gcgatctcac gcgccaatcc gatatgccca agcacatcgg
     1501 ttcgagccgt atccgccaac tcgaggaccg tatcgtcatg gcttcgatcg cactttttca
     1561 cagcccattg cgcgagcgac agaatttgca cgatctgctg gtccgtggca tccaaaccat
     1621 ggatgaggct acgaagccag gataaggacg ctttcattgg atcgggtctt tagcgcagat
     1681 gcagggaggg gagaacggta ggctcagtcg ttttgaggat ctcggacgat aacgccctgt
     1741 gcggtttccg caaattgata ctccggatgg aacttcacgg cggaattgag gttccactcc
     1801 gcctcgaaca aaaccaccgg ccggtctcga acatccacca caagcatggc atcgatctgt
     1861 tgacgaacat gggacagatc caccggttga ccgtccgtac gcgttaccca acgcgcaaga
     1921 tgaaagggca ccgattccag acgaacatcg acaccgtatt ccgaagccag acggtgtttg
     1981 accacctcga gttgcaacaa tcccacggct cccaaaatca tgtctcccgt gcgaatgtcg
     2041 gggggttgat atagttgcac cgttccttct tgggcgagtt gggtggtgcc tcgcacgagt
     2101 tgcttgcgac gcagcggatc gaccatcacg agccgtttga aatactccgg agcgaaactc
     2161 ggaatgggct cgaactccaa tttcgttccg cccgtgagcg tatctccgac ttggaaaaag
     2221 ccagggtcgt acacgccaat gacgtccccg gcgtaggctt cctcgacgat cgtcctttct
     2281 tttgcgataa attgagtggg gttggcgaga cgtacttctc gatccgttcg aacgtgcagg
     2341 accttggtct ttcgatggaa ttgtccggag cagattcgga cgaaagcgac acggtctcgg
     2401 tgagccggat tcatgttggc ttgtactttg aatacaaagc cggtgaattc ttgtcgtccg
     2461 gggtccacca gcccaatgct gctggcccgt ggtccggggg gaggcatcat ctgaatgaag
     2521 gaatcgagaa atgtctcgag gccgaagttg ttgatcgcac tgccaaaaaa catgggcgac
     2581 agacgccctt gcaaaaacga cgatgggtcg aaggcgtcgc ccgcggcatt gagaagatcg
     2641 atatcctgct cgagctgttc gcagccatcc tggccgattt gcgcacgcaa tcgagggtca
     2701 tccaaatctc ccacattcat ggggaccacc atcgctccct gcgtcgcgct cgaaccggcc
     2761 cgagattcgt caaacagcat caacttgtcc aaggtgcgat gataaacccc aagaaataga
     2821 ccggagcg
//
LOCUS       DAOSOR010000684         2826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_35506_length_2826_cov_3.850956, whole genome shotgun sequence.
ACCESSION   DAOSOR010000684 DAOSOR010000000
VERSION     DAOSOR010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35506_length_2826_cov_3.850956"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="PK710_23590"
     CDS             <1..893
                     /locus_tag="PK710_23590"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT12783.1"
                     /translation="MTITFLALTFLTISIMNERARRRNQMVELSRALDLKNRELLRID
                     QMRKQLLAVASHDLKSPLGAVSSYLAGMRDGYLGPLTDEQKHVVQRSLDRLSRLRGFI
                     DDVLAWSSIEQGMLRQEIRPVDIEKLLQRVIEDHRDTALARDIDIRLVVPHPLPKVDC
                     SPERMNQVFENLVSNAVKYSQDGGKVRVEAGREGDDLLVSVLDKGIGIRGEDLERLFE
                     DFFRAPSVRTKVAGTGLGLALVRRIVRAHQGEIWATSELGQGSTFFVRIPITSTQVAR
                     SARLDSQDSESDTVLGDNRN"
     gene            complement(1041..2291)
                     /locus_tag="PK710_23595"
     CDS             complement(1041..2291)
                     /locus_tag="PK710_23595"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HOT12784.1"
                     /translation="MVTSIAMAGCSGSVVENDHAQQEAGSAGAGPSDAAVDVLPSDSP
                     LPTDGPLPSDAPSPTDSPLPTDAPVPPDSSAPDAHCLEPEADFDGDGYTILQGDCDDC
                     NPHINPGAYDYLGDGLDNDCNGTIDDGPHLCDQGLAMKSSESVDGARAIGLCRSTSLG
                     AEGKDKTWGVIRTRYVKADGTEGMNPLSHGLLANFGPETPPEGVTMLALSTGTARAPN
                     HPDYSSNDGANMGTTSNPPPGYPKPIASCPGVILGSCNDPAAFEIDLRVPTNAHSFSF
                     KFNFYSSEFPVYVCSQYNDIFVTMMAPIPSGIPDGNICFGPNLDPISPNTTLLQVCKA
                     QTAGGNQYDCPLGPAGLEGTGFEGKGATGWLQTTAPVEPGSIINLRFATWDSGDAILD
                     SLVLIDDFQWSIELVPKVVTTPVL"
     gene            2521..2727
                     /locus_tag="PK710_23600"
     CDS             2521..2727
                     /locus_tag="PK710_23600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12785.1"
                     /translation="MRFRTAELGRPEAKSRARRSELDQPEALAPMGGQAVSERGKGMG
                     VSRTHGSAARASDLGISEQFVIYG"
BASE COUNT          627 a          730 c          854 g          615 t
ORIGIN      
        1 tcatgacgat cacctttttg gctcttacat tcctaacgat ttcgatcatg aatgagcggg
       61 cccgacgtcg aaatcagatg gtcgaattga gccgcgcgct ggacctgaag aatcgcgaat
      121 tgcttcgtat cgatcagatg cgaaagcagc ttctcgcggt ggcgtcccac gacttgaaat
      181 ctccgctcgg cgctgtctcc agctaccttg cgggcatgcg cgatgggtac ttgggaccgc
      241 tcaccgacga acaaaagcac gtggtgcaac gttcgttgga ccgactatct cggctgaggg
      301 ggttcatcga cgacgttctt gcgtggtcat cgatcgagca agggatgctc cgccaggaga
      361 ttcgacccgt ggacatcgag aagcttcttc agcgcgtgat agaagaccat agagacactg
      421 ccctggctcg cgatatcgat attcgcttgg tcgtgcccca tcccttgccg aaagtagatt
      481 gctcgcccga acgcatgaac caggtattcg aaaacttggt ctccaatgcg gtgaaatact
      541 cccaagatgg gggaaaagtg cgcgtcgaag caggtcgaga aggtgacgat ctgctcgttt
      601 cggttctcga caagggaata ggcatccgtg gcgaagactt ggaacgattg ttcgaggatt
      661 tcttccgtgc gccttcggtg cgaaccaaag ttgcgggtac gggactcggc ctagcgctcg
      721 tgcgacgcat cgtgagggcg caccaggggg agatctgggc cacgagcgaa ctcggacaag
      781 gatccacctt tttcgtgcga atcccgataa cgtcgacgca ggtagcgcga agcgcgaggt
      841 tggattctca agactcggag agcgatacgg tccttgggga taatagaaat taatatgtaa
      901 tatccaatgg ttagaacctg ttggggacag cgatgccgac aggcaggggc gaaaaccatc
      961 cccccgggcg cgttggccga gtcgtggagg gatcccgctt gttatccgtg atcaccctat
     1021 caagtcatcc aagtcgggat tcaaaggacg ggggttgtga ccactttggg taccaattct
     1081 atcgaccatt gaaagtcatc gataagaacg agcgagtcca ggatggcgtc gccagaatcc
     1141 caggtcgcaa accgaagatt gatgatggat ccaggttcca cgggcgctgt ggtttggagc
     1201 catcccgtgg cgccttttcc ttcgaaccct gtgccttcta accccgcggg ccccaacgga
     1261 caatcatact gattgccgcc ggcagtctgg gccttgcaaa cctgaaggag tgtcgtgttg
     1321 gggctgatgg ggtccagatt gggcccgaag cagatgttgc catccggtat tcccgacgga
     1381 attggcgcca tcatcgtgac aaaaatgtca ttgtactgac tacagacgta aacagggaat
     1441 tcggaagaat agaaattgaa tttgaaggag aacgaatgag cgttcgtggg cacgcgaagg
     1501 tcgatttcga acgctgccgg atcgttgcag gagcctagga taacgcctgg acaagaagca
     1561 atcggttttg gataacccgg tggagggttg ctcgtcgtgc ccatgttggc gccatcgttg
     1621 gaactgtagt cggggtgatt gggcgcgcgg gctgttcccg tggacaatgc caacatcgtc
     1681 accccctcgg gtggagtctc cgggccaaag ttcgcaagca gaccatggga taagggattc
     1741 attccttccg taccgtccgc tttgacataa cgagtgcgga tgactcccca ggtcttgtct
     1801 ttgccttcgg cgccaagcga ggtggatcgg caaaggccga tggcccgtgc cccatctacc
     1861 gactcactgc ttttcatcgc caatccctgg tcacaaaggt gaggcccgtc gtcgatggtg
     1921 ccgttgcaat cgttgtccaa gccatctccc aagtagtcat aagctcccgg attgatatgt
     1981 ggattgcagt cgtcgcaatc cccttgcaag atggtatacc catccccatc aaagtccgct
     2041 tccggctcga ggcagtgggc atcaggagca gatgaatcgg gcggtacggg agcatcggta
     2101 ggtaagggcg agtcagtggg agatggggca tcggatggca atggcccatc ggtgggcagg
     2161 ggggaatccg atggcaacac gtcgaccgcc gcatcggaag gtccggcgcc tgccgaacca
     2221 gcctcctgct gggcgtggtc attttcgacg acggagcccg agcatcccgc catggcgatg
     2281 ctggttacca agcaaagact tttccatgca cacacgattg aattctcccg gttctgtcgg
     2341 ctcgagcggc gtggggcgaa gcaaaaagtt ttcgtcatat cttaggtgcg gtcgatgaat
     2401 ttttcggaaa cgagatggcc tatcgccttt tgaaccgttt gcttttgttt cgatgggtgc
     2461 agtgtagcat gccagcgtcg gtcgcctatt gggattcacg cacgacgaca acgtttttga
     2521 ttgcgttttc ggacagcgga actggggagg cccgaggcaa aaagccgtgc tcgacgatct
     2581 gaactggacc agcccgaagc cttggcgccc atgggcgggc aggcggtttc ggagcgagga
     2641 aagggcatgg gcgtgagtcg tacccatggt tccgcggccc gggcttccga cctcggtatc
     2701 agcgaacaat tcgtgatata cgggtagatc ataaacgaga aaggaaaaat atgggcacca
     2761 ggataattta ttactgctgc cggaaatttg gcccacctga aaatacttcg ataagcgctt
     2821 gcgtat
//
LOCUS       DAOSOR010000685         2808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_35814_length_2808_cov_4.208137, whole genome shotgun sequence.
ACCESSION   DAOSOR010000685 DAOSOR010000000
VERSION     DAOSOR010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35814_length_2808_cov_4.208137"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="PK710_23605"
     CDS             <1..701
                     /locus_tag="PK710_23605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12786.1"
                     /translation="SPTIAEADNQPAQPPPSKTLGLEGVLGVSGRLGSMSSGYDSAER
                     AGIQYGAGVFFSPYRELALGLSYTYSGFGAEEFNPSVNDATGKIRRSLHNGMLNIRAY
                     PLRNDTIGLWVGLGAGITWQTATASGAVTTNDFVQPSRSYQVSAGPDAGLALGAALGM
                     DYDVSNDMAVLASLALTNHRLTSEPLDGSTSPAVPGSGSASQLDFRFAFQYRFDLTGT
                     SAPVTATVQTASSE"
     gene            complement(730..909)
                     /locus_tag="PK710_23610"
     CDS             complement(730..909)
                     /locus_tag="PK710_23610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12787.1"
                     /translation="MQAKESNGLCGQRNRCVIVGDDEYRLGLARSAFAGTRPQAADDL
                     ADGIVAEQLSSTEFP"
     gene            complement(1225..>2808)
                     /locus_tag="PK710_23615"
     CDS             complement(1225..>2808)
                     /locus_tag="PK710_23615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12788.1"
                     /translation="AVAQWRIVHGKVCSPTPRDHAAWQDFLSDATVPGDGSSKAPYWH
                     ARPEPNFGGGKVRWFHRFGSTSNAYIHTNPSDNGADPYEVAVNTRRKYEAMYPFTYFR
                     RKNREFNFLFMPWAISRRYFDIERTYNWNATAKAAQYYASFGPERYAEMAASDDWLRP
                     YLMASKEAFDMLAGAVMSPQPGPYYVSSERKINGYIKGSIYDTTDNPSGFASKSFDIP
                     LGLGRFIDDDMDSGPFGGGSWKYQEWMNRAGFELEKAFALAMLTDGRPPVYSPNREMF
                     LDPRTQRLNFYASMPQAMSRFIGGIMAEDWESVGMHYICTQRDPLANTCVSGQAEMFD
                     ITKPTLPVRPANAAILFPNIGFRQQSVAAILAAVHSRRTGDMSLLNKTRIWIEGVDGT
                     VGTTGFPDPKEQIRFFNPMSGMTYIARRYGTETLQKVDNTGATVPVKEVEKGIGARML
                     QLANWLMVYTYKVERDAATNDPVVNEFGQVQLKLDANGQPMLLSTDPANTKLSALIKY
                     VGHIDGMRQIGLLIGSGPL"
BASE COUNT          600 a          873 c          729 g          606 t
ORIGIN      
        1 cttcacccac cattgccgag gcggataacc aaccggccca accgcccccc agcaaaaccc
       61 tcggcttgga aggtgtcctc ggtgtttccg gacgccttgg ctccatgtcg agcggatatg
      121 actccgcgga acgagcggga attcaatatg gcgcaggtgt ttttttctcg ccctatcgcg
      181 aactcgcgtt gggtctttct tacacatata gcggattcgg agccgaggaa ttcaatccct
      241 ccgtcaatga cgctaccggc aaaatccggc gttccctgca caatggaatg ctcaatatcc
      301 gcgcatatcc cctgcgcaac gacactatcg gcctatgggt cggactcgga gccggcatta
      361 catggcaaac cgccaccgcc tctggcgccg tcacgaccaa cgacttcgtc caaccttcac
      421 gctcctatca agtgagtgcc ggtcccgatg ccggcctcgc cctcggcgcc gccctcggaa
      481 tggattacga tgttagcaac gatatggccg tcctcgccag cctagcgctc accaatcatc
      541 gtcttacgag cgagcctctc gacggcagca cttcccctgc cgtgcccggc tccggctccg
      601 ccagccaact cgacttccgc ttcgctttcc aatatcgctt cgatctcacc ggcacatccg
      661 ctcccgtcac cgctacagtc caaacggctt cctcggaata gccgaaagaa gccgtccccg
      721 actttttcgc tatgggaact ccgtgctcga gagctgctcg gccacgatcc catccgccaa
      781 atcatccgcg gcttgcggtc gcgtccccgc gaaagcgctc cgcgccaaac ccagtcgata
      841 ctcgtcatct cctacaatca cgcatctgtt gcgttgaccg cacaagccgt tcgattcctt
      901 cgcttgcatc gcatcgcata ggctagagct tcgccctatc cccacaaaaa ccgacaaggc
      961 gaaaagaatg cttatttctt gtcatcgaga agatttgtct tgcggtggtg ggaaaggatg
     1021 gtgcgcgcgg agcatacagt gctctgctgt ggctcgcttt tctgcccaag cctggctacg
     1081 cctttcgccc gccaaccgat ggggtttggg caaacgtatc cctcgaagta gggaggtcat
     1141 caggacaatt tcttgtggaa tctcagaagg atagacgggg gaaggagcct cggcgaaaga
     1201 gccgtccgag gctccttggg tctctcaaag cggaccggag ccaataagca agccgatctg
     1261 ccgcattccg tcaatatgac ccacgtactt gatcaaggca ctgagtttcg tattggcggg
     1321 gtcggtgctc aatagcatcg gctgcccatt ggcgtcgagc ttgagttgca cttgtccgaa
     1381 ctcgttgacc accggatcgt tggtcgccgc atcgcgctcg accttgtagg tatacaccat
     1441 cagccagttc gccaattgaa gcattctggc gccaatgccc ttctccactt ccttgaccgg
     1501 caccgtcgcg cccgtattat caaccttttg caaggtttct gttccgtaac ggcgggcgat
     1561 ataggtcata ccggacatcg gattgaagaa ccggatttgt tctttgggat ccgggaagcc
     1621 agtggtaccg accgtgccgt ccacaccctc gatccaaatc cgcgttttgt tgagcagcga
     1681 catatcaccc gttcgacggg aatgaaccgc tgccaagatg gctgcaacgc tctgctggcg
     1741 gaagccgata ttggggaaca ggatagcagc gttggccggg cgaacaggca gggtgggttt
     1801 cgtaatatcg aacatttccg cctgtcctga cacacacgta ttcgcgagtg gatccctctg
     1861 cgtgcagata tagtgcattc ccacgctctc ccagtcctct gccatgatgc cgccaatgaa
     1921 tcggctcatc gcctgcggca tcgaagcata gaagttcaag cgctgggtac gcggatcgag
     1981 gaacatctca cgattgggcg aatataccgg aggtcgaccg tcggtcaaca tggcaagcgc
     2041 aaacgccttc tccagttcaa atcccgcgcg attcatccat tcctggtatt tccaggaacc
     2101 accaccaaac ggaccactat ccatgtcatc gtcgatgaac cggcccaaac cgagcgggat
     2161 atcgaaggac ttcgaagcaa aaccactggg attatccgtt gtatcgtaaa tactcccctt
     2221 gatgtagccg ttgattttgc gttccgaaga aacgtaatag ggacccggct gcggggacat
     2281 gacagcccca gccaacatat caaaggcttc cttactcgcc atcaagtacg gccgaagcca
     2341 atcgtccgac gccgccattt ccgcatagcg ttccggtccg aagctggcgt aatactgagc
     2401 cgctttggcc gtggcattcc agttgtacgt tcgttcgatg tcgaagtatc gacgagaaat
     2461 cgcccacggc atgaacaaga agttgaattc tcggttcttt cggcggaagt aggtgaacgg
     2521 atacatcgcc tcgtacttgc ggcgcgtgtt gaccgccacc tcataaggat ccgcaccgtt
     2581 gtcgctcggg ttcgtgtgaa tataagcatt cgacgtcgaa ccaaaacggt ggaaccagcg
     2641 taccttgccg cctccgaagt tcggttcggg gcgagcatgc caatacgggg ccttggaact
     2701 cccatcgccc ggcaccgtcg catcgctgag gaagtcttgc caagccgcat gatcgcgcgg
     2761 agtcggtgag cataccttac cgtgaacgat acgccactga gccacagc
//
LOCUS       DAOSOR010000686         2804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_35879_length_2804_cov_5.317934, whole genome shotgun sequence.
ACCESSION   DAOSOR010000686 DAOSOR010000000
VERSION     DAOSOR010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35879_length_2804_cov_5.317934"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(592..2727)
                     /gene="pta"
                     /locus_tag="PK710_23620"
     CDS             complement(592..2727)
                     /gene="pta"
                     /locus_tag="PK710_23620"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOT12789.1"
                     /translation="MARSLYISAAEPHSGKVLIALGLMGVLDRTIHRIGFFRPVAVPY
                     RLTGSETTVPDRDVHLMREVFSLDGEVSEMFGVTSKQAETMTAAGQQGALLERILEAY
                     KQLEGKVDFVICEGTDFLSTTPSIEFDFNTEVARDLNAPVVLVVDGSSNEADEIFSKA
                     KIGFDGFRAKGCEVLAVFVNKVRGSGCEMLRAELAPRLNDAGIALAAFLPYETTLAMP
                     RMDEVTAQLGGRVLYGKNYLHNKVGKTVVASGSVETMIPLLDNNLLLVTHTDRHDAIL
                     MALVSLMSHTAPNIGGIVLSGEQELAAPVRELIEGLPGPRIPIVRSPKPTYETVLALT
                     RVRAGIEPGHARELEVARQQFETSVDTEALTKAIRLSAPRIMTPKLFKYELITRARAA
                     KKHIVLPEGEEERILEAADALLRLRAVELTLLGNPENVLAKAGQLGISLDGARIENPK
                     TSAWLDEFVAIYQRARAHKGMTFEAAREYVLEPTVFGTLMVHTGHADGLVAGATTSTA
                     MTIRPAFQVIKTRKGFSIVSSVFFMCLEDRVLVYGDCAVNPDPTASQLAEIALASAET
                     AQDFGIEPIVAMLSYSTGESGQGADVEKVKEAVKIARAARPSLLLEGPLQYDAAVDAS
                     VAKTKLPGSQVGGRATVFIFPDLNTGNNTYKAVQRSARAIAIGPVLQGLNKPVNDLSR
                     GCLVEDIINTVAITAIQAQGARGGGASHS"
BASE COUNT          550 a          831 c          833 g          590 t
ORIGIN      
        1 gcaggaatcc acaatgattt gccccgccca aagctcgagc tggaagtcga gctgtacggc
       61 tgtcaccgaa ggtgtcgatt cgagattgga aaccgagacg cggcacgtca tgccctgggg
      121 gagcttcagc caatcccctt gtatttgtga gggtatccca tgctcccgga gcaacccctg
      181 gagcatggca tgatggctgt gcgcagtgtg gtgcaagaat gctctcctga aacgggcttt
      241 ggggtggcgt aaggtttggg gaaaacaagg tggccgcttc gaaaatatcc ccttcgtctt
      301 tgccgggggg tcgtcgcata aggtagcagc cacggcaacc gacgaggtgc gggaggggcg
      361 ggaaaccgct cgaaatgact atgttccgcg agatgcaggc ggaaccgaac gtgcgcttcc
      421 cgtacgacag gtaacggata ggttgtggcc gctggagtca gactctagat aaataataat
      481 aaattcaata tgttacacta gttttgcaga gtcgaaaacg aatcgcccgc tgccgtacga
      541 cgctcgctcc ggtgaccgaa cagccgaaat agctatttcg ccaaaccgat cctacgagtg
      601 tgaagctccc ccgcctcgcg ccccttgagc ctggatcgcg gtgattgcca cggtgttgat
      661 gatatcctcg acaaggcagc cgcggctcag gtcgttgacc ggtttgttga gcccttgaag
      721 caccggtcca atcgcgatcg ctcgcgccga acgctgtacg gctttgtagg tgttgttgcc
      781 ggtgttgaga tcggggaaga tgaacacggt tgcacgaccg ccgacctgtg agcctggcag
      841 cttggttttc gccacgctcg cgtccacggc cgcgtcgtat tgcagtggtc cttcgagcag
      901 aagcgaaggc cgtgccgcac gtgcgatctt cactgcttct ttgaccttct cgacgtctgc
      961 gccctgccct gactcgccgg tcgaatacga aagcatcgcc acgattggct cgatcccaaa
     1021 gtcctgcgcg gtttcggcgc tcgccaatgc gatctcggca agctgtgaag cagttggatc
     1081 gggattgacc gcgcagtcac cgtaaaccag cacccgatcc tcgaggcaca tgaaaaaaac
     1141 cgaggagacg atgctgaagc ccttgcgcgt tttgatcacc tggaacgcgg ggcggatcgt
     1201 catcgccgtc gaagtcgtgg cgcccgctac cagaccatcc gcgtggccgg tgtggaccat
     1261 caacgttcca aaaaccgtgg gttcgagtac gtactcacgc gccgcctcga acgtcattcc
     1321 cttatgagcg cgggcgcgtt ggtagatagc gacgaattca tcgagccacg cgctcgtttt
     1381 cgggttttcg atccgtgcgc catcgagcga aataccgagt tgaccagcct tggcgagcac
     1441 gttttccgga ttgccgagca gcgttagctc cacggctcgc agccgaagca atgcatccgc
     1501 cgcctcgagg atacgctcct cctctccttc gggcaggacg atatgtttct tagcggcacg
     1561 ggcacgcgtg atcagctcat acttgaagag cttcggcgtc atgattcgcg gtgcgctcaa
     1621 acgaatcgcc ttggtcagcg cttccgtgtc cacgctggtc tcgaactgct gacgggccac
     1681 ctcgagttcg cgcgcatgac cgggctcgat tcccgcgcgc acccgggtca gcgcgagcac
     1741 cgtctcgtag gttggctttg gcgaacgcac gatcgggatc cgcggtcctg gcagcccttc
     1801 gatcagctct cgcaccggcg ccgccagctc ctgctcgcca gaaagcacga tccctccgat
     1861 gttgggcgcg gtatggctca tcagggagac gagcgccatc aagatggcgt cgtgccgatc
     1921 ggtgtgcgtg acgagcagca agttattgtc gagcagcggg atcatcgttt cgacggagcc
     1981 cgatgccacg acagtcttgc ccactttgtt atgcaaatag ttctttccgt aaagcacacg
     2041 accgccgagt tgcgcggtga cctcgtccat gcgcggcatc gcgagggttg tctcgtaggg
     2101 taagaaagcg gcgagcgcga tccccgcatc gttgagccgt ggtgcgagtt cggctcgtaa
     2161 catttcacat cctgatccac gcaccttgtt cacgaacacc gcgagcacct cacagccctt
     2221 ggcacggaac ccgtcgaatc cgatcttcgc tttcgagaaa atttcatccg cctcgttcga
     2281 agagccgtcg accacgagca ccacgggtgc gttgagatcc cttgcgactt cggtgttgaa
     2341 gtcgaattcg atcgacgggg tggtggagag aaagtcggtt ccttcgcaga tcacgaagtc
     2401 cacttttccc tcgagttgtt tgtaagcctc gaggattcgt tcgagcagcg cgccttgctg
     2461 tccagccgcg gtcatcgttt cggcctgctt gctcgtcaca ccaaacatct cgctcacctc
     2521 gccatcgagc gagaagacct cacgcatcag gtgaacgtcg cggtcgggaa ccgtggtttc
     2581 ggaacccgtc agtcgatacg gcacagcgac cggacggaag aaaccaatgc gatggatcgt
     2641 gcgatcgagg acccccatga gtccgagtgc gatgaggacc ttgccgctgt gtggctcggc
     2701 ggcgctgatg tagaggctcc tagccatttt caactccccg ctggcggatc gtgcgcttga
     2761 ttgacgatat catgcacgat acgagggcgg ctgctcaatc gttt
//
LOCUS       DAOSOR010000687         2793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_36058_length_2793_cov_3.789993, whole genome shotgun sequence.
ACCESSION   DAOSOR010000687 DAOSOR010000000
VERSION     DAOSOR010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36058_length_2793_cov_3.789993"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1928)
                     /locus_tag="PK710_23625"
     CDS             complement(<1..1928)
                     /locus_tag="PK710_23625"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HOT12790.1"
                     /translation="MGWDRVRRCGKPGPWLLFASMVGGCGFDRSDRWDDLRPTSMMTC
                     REGDLQCSLVLEVCVSNGAGGTRWTVLDDCKAQGKVCAPSLLACAHCFPDERICDGQQ
                     VLVCTSDGTKRVVIDDCEGKRGVACRSGACTDLCAEAAVKQSNVGCEYWGVDLDNANI
                     NATSNAAAQQYAIVVSNPQPDLSAEVTIEQDDGPVGSPNDPMVVSSATIYPGNLRVFK
                     LGPREVDGSPPGQFDTGPGTAHTRAAYRVLSSVPVVAYQFNPLDNVNVFSNDASLLKP
                     VEAVTYTPGLLDLQYIGLGWPQTIAHTDDPETNFNPANPTNLRAFLTVVGTQPDTRVR
                     IAPSTVVVAGMGIEQTRPGQYIEAVLQPFEVLNLETGDFNADFTGTLVEADGPVLAFS
                     GSEASDAPYFDRLANRFCCADHLEEQLDPLRTAGTSFFAAHAPNRSAAVSQAGASLLG
                     VVPEPEFFRILSVADRPVHIQTTLPEPDDKLELPGRGAYLDLIAYRDFHIDADGPIML
                     ASVQASQDAAGVRRGLPGGDPSLLIIPPIEQYRANYVFLTPDKYAFDFVVIVASTEAL
                     VTLDGQDVMEAGCARAAADGLTDQERGSSHSPAWVYRCQLSFPVVLPDAEAPNNVLPG
                     IQNDGVHRIEASMPVLALV"
     gene            complement(1932..>2793)
                     /locus_tag="PK710_23630"
     CDS             complement(1932..>2793)
                     /locus_tag="PK710_23630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12791.1"
                     /translation="SIGYEFSLTAYPGNLSLYALAGIENRKSPTAVFTAYAMGFLQGI
                     STLPGQTTKDIFIPMTKPLDQAVVLSVEPPSAGPKGPDRLIASVAVKLGNEGYAIFPN
                     GQRVALLANTGDLPFVGMPGLTDALAGSRYVSSAKAVTGANAGTPLSVIEKYLSNDAS
                     LPIQMAGFVQVPVLQEPAAGQPFDGRHLRIAYAPGGASVDISVVRIQAGAGLVEWLVA
                     IPGSKSPVELPDLASLGLGLPPGPLSIFVYGGHVGDGSFDYGTLVYRQLDARGWTAYA
                     YDVFHRYGGN"
BASE COUNT          662 a          886 c          718 g          527 t
ORIGIN      
        1 accagggcca gtacgggcat gctggcttcg atgcgatgga caccatcgtt ttgtatgccc
       61 ggaagcacat tgttgggcgc ttccgcatcg gggagcacca cgggaaaaga aagctggcaa
      121 cgatacaccc acgcgggcga atggctcgaa ccacgttctt gatccgtcaa accatccgcg
      181 gcggcacgcg cacatcccgc ttccatgaca tcttgtccgt ccaacgtcac caacgcctcg
      241 gtggatgcca cgatgaccac aaaatcaaac gcgtatttgt caggcgtgag aaatacatag
      301 ttggcgcgat actgttctat cggcgggatg atgagaagac tcggatcacc accaggcaac
      361 cctcttcgca cgcccgctgc gtcttggctc gcttggacac tcgcaagcat gatcggaccg
      421 tcggcatcga tatgaaaatc ccgataggca atcagatcca agtaggcacc gcgtccgggt
      481 aactcgagct tgtcatcagg ctccggcagc gtcgtctgaa tgtgaacggg tcgatcggct
      541 accgacaaaa tcctgaaaaa ttccggctcc ggcacgactc cgagcagcga tgcccccgct
      601 tgcgataccg ccgcggaacg attgggggcg tgagcggcaa aaaaactcgt tcccgccgtt
      661 cgaaggggat caagctgctc ttccaagtgg tccgcacaac aaaaccgatt ggccaaccga
      721 tcgaagtaag gagcatcgga cgcttccgaa cccgaaaaag ccagcacggg accatccgct
      781 tccaccagcg ttcccgtaaa atccgcattg aaatccccgg tttcgagatt cagaacctcg
      841 aacggttgca gcaccgcttc gatgtactgc ccaggcctgg tctgctctat acccatgccg
      901 gctaccacga ccgtcgatgg cgcgatgcga acacgagtgt ccggttgggt gcccaccacc
      961 gtcaaaaatg ctcgaagatt cgtgggattc gctggattga agttggtctc cggatcgtcg
     1021 gtatgcgcta tcgtttgcgg ccaacccaaa ccaatatact gaagatcgag caaaccaggc
     1081 gtatacgtca ccgcttcgac cggtttgaga agcgatgcgt cattcgaaaa aacattgacg
     1141 ttgtcgagcg gattgaattg ataagctacc accggtaccg aagagagaac gcgatacgcc
     1201 gctcgcgtat gcgcagtgcc cggacccgta tcgaattgac ccggcggact gccgtccact
     1261 tcccgagggc ccaacttaaa tactcgaaga tttccaggat aaatcgtcgc ggaagagaca
     1321 accatcgggt cgttgggcga acctaccggc ccatcgtctt gctcaatcgt tacctccgcc
     1381 gacaaatcag gctgggggtt cgataccacg atggcatact gctgcgccgc cgcattgctc
     1441 gtcgcattga tgttggcgtt gtccaaatca acaccccaat actcacagcc cacattgctc
     1501 tgttttaccg ccgcttccgc acataaatcg gtacaagccc cggaccgaca ggccactccc
     1561 cgctttccct cacagtcgtc gatgaccacc cttttcgttc catccgaagt acaaaccaat
     1621 acttgctgcc catcgcatat ccgttcgtcc ggaaagcaat gagcacacgc aagaagcgag
     1681 ggagcacata ccttgccttg cgccttgcaa tcatccaata ccgtccatcg cgtgcccccc
     1741 gcgccgttgg atacacatac ttccaacacc aaggagcatt gcaggtcccc ctcgcggcac
     1801 gtcatcatcg acgtgggtcg tagatcatcc caacggtccg aacggtcgaa accgcaaccg
     1861 cccaccatgc tcgcgaacaa gagccatggc cccggcttcc cacaacgtcg tacgcggtcc
     1921 caacccatcg attagttgcc cccataccga tggaaaacgt cgtaggcata agccgtccag
     1981 ccccgtgcgt cgagttgacg atacaccagc gtgccgtagt caaaggaacc atcgcccacg
     2041 tgcccaccat agacaaaaat cgataacgga cccgggggca gaccgagccc gagcgacgca
     2101 aggtcgggta gctccacggg tgatttcgaa cccggaatgg cgaccaacca ttcgacgaga
     2161 ccagctccgg cctgaatgcg cacaaccgaa atatcgacgc ttgcccctcc tggcgcatac
     2221 gctattcgca gatgcctgcc atcgaagggt tgacccgccg ccggctcttg cagcacaggg
     2281 acctgaacga aacccgccat ctgaatagga aggctcgcgt cgttggataa gtacttttcg
     2341 atgaccgata acggtgtccc cgcattggct cccgtcaccg ctttggcaga ggaaacataa
     2401 cgagaacccg caagagcatc cgtcaaccct ggcatcccga caaacggcaa atcccccgta
     2461 ttcgcgagca gcgcaacccg ctgaccgttg ggaaagatcg cgtacccttc gttgcctaac
     2521 ttgactgcca cactggcgat caaacgatcc ggccctttgg gccccgccga tggcggttcg
     2581 accgacaaca ccaccgcctg atctagcggc ttcgtcatgg gaatgaaaat gtccttggtc
     2641 gtttgtcctg gcagggtcga gatcccttgt aggaacccca tcgcgtaggc cgtgaacacc
     2701 gcggtgggtg acttgcgatt ctcgattccc gcaagagcat aaagcgaaag gttgcccggg
     2761 tacgctgtca aagaaaactc gtatccaatg ctg
//
LOCUS       DAOSOR010000688         2785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_36206_length_2785_cov_3.044689, whole genome shotgun sequence.
ACCESSION   DAOSOR010000688 DAOSOR010000000
VERSION     DAOSOR010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36206_length_2785_cov_3.044689"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            361..678
                     /locus_tag="PK710_23635"
     CDS             361..678
                     /locus_tag="PK710_23635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12792.1"
                     /translation="MVIEAYEWLVVLSSDEDVQRCARERDENRKLNEIELWLTREEGR
                     EEGREQGKREVIQRILSLRSIELTPSDHDALMACHDITTLDKLLERALLMQPGQALIE
                     GEP"
     gene            complement(887..1885)
                     /gene="gmd"
                     /locus_tag="PK710_23640"
     CDS             complement(887..1885)
                     /gene="gmd"
                     /locus_tag="PK710_23640"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOT12793.1"
                     /translation="MTKRALITGITGQDGSYLAELLLSKGYEVHGIIRRSSSFNTERI
                     DHLYRDRHDEDAKMILHYGDLNDASSLQKVLTDSEPDEVYNLAAQSHVRVSFDVPEYT
                     AEITALGTIRLLEAIRRAEMKPRFYQASSSELYGKVVETPQTEKTPFYPRSPYAAAKA
                     FAFHVTRNYREGYGMFAVNGILFNHESPRRGETFVTRKITRAVGRIKHGLQDKLYLGN
                     LEAKRDWGYAGDYVEAMWMMLQHPEPEDFVIATGQTHAVRELLDEAFGYAGLDWRKFV
                     EIDPRYHRPTEVDLLLGDASKARRLLGWEPKVSFKELVRKMVDADMALAKREKRVG"
     gene            1964..>2785
                     /locus_tag="PK710_23645"
     CDS             1964..>2785
                     /locus_tag="PK710_23645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOT12794.1"
                     /translation="MAKTSKQERVFVAGHTGLVGSSVMRRLHGLGYDDVVGRDVAQLD
                     LTDAVATRAFFEEMRPSYVVLAAAKVGGIHANNTFPADFIRINLQIQTNVIDAAHRTG
                     VKKLLFLGSSCIYPKHAPQPMKEEHLLTGPLEPTNDAYAIAKIAGILMTQAYNRQHGT
                     NFIAAMPTNLYGPGDNFDLNTSHVLPAMVRKFVEAKDQKKAEVELWGTGSPRREFLFV
                     DDLADALVFLLENFDATGERDNFVNVGVGEDISIDALAALVREVVGYEGKIAWNAS"
BASE COUNT          665 a          777 c          806 g          537 t
ORIGIN      
        1 ccagcgatca cgacgcgttg atggcctgcc atgacatcac caccctggat aagttgctgg
       61 aaagagcctt gctcatgcag ccgggacagg cgcttttcga aggcgaatcc tgaccgggaa
      121 gacacagaga gcaggcagag cggttcacca cagagataca gagggcacag agcggagaca
      181 aaagcggaga aaaacgggag gaccgtcgcc agcacgacaa atggcaacgt ccggtacgat
      241 taaatcgcgc cgccaaccgc gggcaacgct gtaggggcag gtattatacc tgcccatcgt
      301 acaaggtttt taggagccaa aacggatgag gaaagacgca aggtgtcgca ggaggatccc
      361 atggtgatag aagcctatga atggctggtg gtactctcga gcgacgaaga tgtgcaaagg
      421 tgcgcgagag aacgcgacga gaatcgtaag ctgaacgaaa tcgaactctg gctgacgaga
      481 gaagagggga gagaagaggg aagggagcaa ggaaaacggg aagtcatcca gcgcattcta
      541 tccctgcgtt cgatcgaatt gacccccagc gatcacgacg cgttgatggc ctgccatgac
      601 atcaccaccc tggacaagtt gctggaaaga gccttgctca tgcagccggg acaggcgctt
      661 atcgaaggcg aaccctgaca tctgatggga aaggttcgaa agcagggggc ggattcacca
      721 tggggataat gaaagccaga aacgccacgg ctaggtaggg ccggaccatt gccagctacg
      781 cttcgggttg aggaaagaga tggggccaca acccagaaaa ggggaggagt aaaatataat
      841 aaatacatac agttgcaact gtcgcacaga gcgcaggaaa tacccctcac cccacccgct
      901 tctcccgctt cgccaacgcc atatccgcgt ccaccatctt gcgcaccaac tccttgaacg
      961 acactttcgg ctcccagcca agcagccgac gcgccttgct cgcatccccc aacagcaaat
     1021 ccacctccgt cggacgatga taccgcggat cgatctccac aaacttcctc caatcgagcc
     1081 cagcataccc gaaagcctcg tcgagtaact cccgcaccgc atgggtttgc cccgtcgcaa
     1141 tcacgaagtc ctccggctcg ggatgctgta gcatcatcca catcgcctcc acataatccc
     1201 ccgcgtatcc ccaatcgcgc ttcgcctcca ggttccccaa gtacaactta tcttgcagtc
     1261 cgtgcttgat ccgccccacg gcgcgcgtga tcttgcgcgt cacgaaggtc tcgccccgac
     1321 gcgggctttc atggttgaac aggatcccat tgaccgcgaa catcccgtag ccctcgcgat
     1381 aattgcgcgt cacgtggaag gcaaaagcct tggccgcggc gtagggactg cggggataaa
     1441 aaggcgtttt ttccgtttgg ggcgtctcca ccactttgcc ataaagctcc gacgaactgg
     1501 cttgataaaa gcgcggcttc atctccgcgc gacgaattgc ctcgagcaac cggatcgtgc
     1561 ccaatgctgt gatttcagcc gtgtattccg ggacgtcgaa cgaaacccgg acatggcttt
     1621 gggcggcgag gttgtagact tcatccggct cgctatccgt caacaccttc tgaagcgacg
     1681 atgcatcgtt caagtcaccg taatgtaaga tcattttggc gtcttcgtca tgacggtcgc
     1741 ggtacagatg atcgatcctt tcggtgttga acgaactgga ccgacgaatg atcccgtgta
     1801 cttcatagcc cttggaaagg agcagttccg cgagataaga gccgtcttgg ccggtgatgc
     1861 ccgtgatgag tgcccgcttg gtcatggcgg ggcatcatgc tcgaactcgc tctccaattc
     1921 cagtcccttg cgttgtggtt cgagaaggag taggcccgaa cggatggcaa agacgagcaa
     1981 gcaagaacgt gtattcgtgg ctgggcatac gggattggtg gggtcgtcgg tcatgcggcg
     2041 tcttcacggc ctgggatatg acgatgtggt ggggcgagac gtggcccagc tcgatctcac
     2101 ggatgccgtg gcgacaaggg cgtttttcga ggaaatgagg ccgtcgtacg tggtgcttgc
     2161 cgcggccaag gtggggggaa tccacgccaa caacactttt cccgcggact tcatccgcat
     2221 caacttgcaa atccagacga atgtgatcga tgccgcccat cgcacggggg tgaaaaagct
     2281 cctttttcta ggaagttcgt gcatttatcc caagcatgcg ccccagccga tgaaagaaga
     2341 acaccttctt accggcccgc tcgagcccac caacgacgcc tacgccattg ctaagattgc
     2401 cggcattctg atgacccagg cgtacaatcg tcagcacggc acgaacttca tcgcggcgat
     2461 gcccacgaat ctctatggcc ccggggacaa cttcgatctg aatacttcgc atgtgcttcc
     2521 cgcgatggta cgaaaattcg tggaagccaa agaccaaaaa aaggcggaag tggaactgtg
     2581 gggcacgggc tcaccgcgtc gggagttcct tttcgtggac gatctcgccg acgccctcgt
     2641 ttttctgctg gaaaacttcg atgctacggg ggagcgagac aatttcgtga acgtgggggt
     2701 gggtgaggat atatcgattg acgcgctagc ggcattggtt cgggaggtgg tgggctacga
     2761 agggaaaatt gcgtggaatg cgtcg
//
LOCUS       DAOSOR010000689         2763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_36582_length_2763_cov_4.021787, whole genome shotgun sequence.
ACCESSION   DAOSOR010000689 DAOSOR010000000
VERSION     DAOSOR010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36582_length_2763_cov_4.021787"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..232
                     /locus_tag="PK710_23650"
     CDS             80..232
                     /locus_tag="PK710_23650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12795.1"
                     /translation="MNCILACVLDEETEDVVACVGQCDTDYPVGKALYDAAIGCVDEH
                     CMAQCE"
     gene            504..707
                     /gene="rpmE"
                     /locus_tag="PK710_23655"
     CDS             504..707
                     /gene="rpmE"
                     /locus_tag="PK710_23655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014697411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOT12796.1"
                     /translation="MKENIHPTYVAAKITCACGASFETRSSKGDYSVDVCAMCHPYYT
                     GKMKLVDTQGRVERFRRKYATKA"
     gene            740..1885
                     /locus_tag="PK710_23660"
     CDS             740..1885
                     /locus_tag="PK710_23660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOT12797.1"
                     /translation="MSSERLSTCATEARPYIGGQAVIEGVMMRSPRSYAVVLRRRDGT
                     LTVKERAMTDTRKGVLAWPLVRGVATLVEALRLGTGALRFSAEHYEADLEAEEKKKGP
                     GVIASCLSAFRTITGAIVGIATSDLDAQPSGGQTKSGSSMTFVAMLFALLLFVALPQA
                     AAAGASRLLHIEVDIRSPAFQVLTGIFKLCIVLGYMLLMRRIPEVRRVFMYHGAEHKA
                     IATYEAAEALTVDNARSKTRFHPRCGTTFLVMVVFVSILVFSGIGPLLPRLNLGGVVE
                     NILFFLMKLPFLPVIAAITYEIQRWTARYCLRGPLRLVLYPGFAVQGITTLEPDDTQL
                     EVALASLRATLWREQAQEVPNATPEVTQYRDFSHLMEQPAYGASNRA"
     gene            1900..>2763
                     /gene="prfA"
                     /locus_tag="PK710_23665"
     CDS             1900..>2763
                     /gene="prfA"
                     /locus_tag="PK710_23665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOT12798.1"
                     /translation="MLPRDKLEALERRSIEIEEALCHPEVANDYQKVQKLNKERSELL
                     PVVEAYQQVRKIESQLEEDRAALQDPDLRELAEAEIPSLEEQLTALEQRVELLLLPVD
                     PNDQRNTILEIRGGEGGEEAALFAADLFRMYVRFAESRRWKVEVLSLSEASAGGYKEV
                     IALVTGHHVYSHLRYEAGVHRVQRVPVTEAQGRIHTSTATVAVLPEAEDVEIQIDDKD
                     LRFDIAASGGPGGQGVNTTNSAVQITHLPTGLIVKCQDERSQIKNKAKAMKILRSRLL
                     DMEQQKRDAEVS"
BASE COUNT          573 a          697 c          876 g          617 t
ORIGIN      
        1 tatcgcgctg gactgccact caacgaattg aaggggcgcg aaacctacgt tgaacgcgga
       61 aatatgcgat gatttctatg tgaattgcat attggcctgt gtgctcgacg aagaaacgga
      121 ggatgtggtc gcatgcgttg gacagtgcga tacggattat ccggtgggaa aggcgttgta
      181 tgatgcggcc attgggtgtg tggacgagca ttgtatggcg caatgcgagt gaccgatggg
      241 cgaggagcgt gcgagccgag gtcgggagga gatcggctca gattttgtaa tcactggaaa
      301 acattgaggt ttattggaga tccccatgcc ggggatgaac aaaaacgcat gaaaacggca
      361 aggaatggaa ctcggccttg cgcgcgtatc gcttgacgcg taaagtgcgc ccccttgctt
      421 cccgtgccat catggatggc ctaggttttg ccgaagattc gtccggaatt gcgtggcaat
      481 gccggtgatt ggatgaggtc gtgatgaaag agaatattca cccaacgtat gttgccgcca
      541 agatcacgtg cgcttgtggg gccagcttcg agactcgttc gagcaaggga gattactcgg
      601 tcgacgtttg cgcgatgtgc catccttact acacgggcaa aatgaagctg gtggacaccc
      661 agggtcgtgt cgaacgcttc cgtcgaaagt acgccaccaa ggcctagcct tctcccactt
      721 tttgcctaac aacttcaaga tgagttcgga acggttatcc acctgcgcca ccgaggcgcg
      781 tccctatatc ggcgggcaag ccgtcatcga aggggtcatg atgcgttcgc ctcggtccta
      841 tgcggttgtg ctgcgacgtc gtgacggcac gttgacggtc aaagagcgtg ccatgacgga
      901 tacacgcaag ggggtgttgg cgtggccgct ggttcgtggc gtggcaacgt tggtcgaagc
      961 gcttcggctt ggaacagggg cgcttcgatt ttcggcggag cattatgaag cggacctgga
     1021 agcggaagag aaaaagaaag gtccgggtgt gatcgcatcc tgtctgtcgg cgtttcgcac
     1081 catcaccggt gcgatcgtgg gcattgccac ctctgatctc gatgcacagc ccagcggtgg
     1141 gcagaccaaa tccggttctt cgatgacgtt tgtggcgatg ctctttgccc ttcttttgtt
     1201 cgtggccctt cctcaagccg cggctgcggg agcgagtcgt ttgctgcata tcgaagtgga
     1261 catccgctcg ccagctttcc aggtgttgac cggtatcttc aagctctgca tcgtcctcgg
     1321 atacatgctg ctgatgcgtc gaatccccga ggtgcgacgg gttttcatgt atcacggggc
     1381 ggagcataag gcgattgcca cctacgaggc ggccgaggcg ttgacggtgg acaatgcgcg
     1441 gagcaagacg cgttttcatc cccgatgtgg gacgacattt ttggtgatgg tggtcttcgt
     1501 atcgatcctt gtgttttcgg ggattgggcc attgctaccc cgtctgaacc ttggcggcgt
     1561 cgttgagaac atattgtttt tcttgatgaa attgccgttt ctgcccgtga ttgccgccat
     1621 cacgtatgag atccagcgtt ggacggcgcg ctattgtctt cgcggtccgc tgcgcttggt
     1681 gttgtatccg ggcttcgcgg tgcagggcat caccacattg gagccggacg acacccaact
     1741 cgaggtggct cttgcttctt tgcgcgcaac cttatggcgc gagcaggccc aagaggtgcc
     1801 gaatgccacg cccgaggtca cgcaataccg tgacttctcc catctgatgg agcaacccgc
     1861 ttatggcgcg tccaatcgcg cgtgacgtcg tgagggtcca tgctgccgcg tgacaagctt
     1921 gaagctctgg aaagacggtc catcgagatc gaggaagcgt tgtgtcaccc ggaggtggcg
     1981 aacgattatc aaaaggttca gaagctcaac aaggagcgaa gcgagttatt gccggtggtc
     2041 gaggcctatc aacaggttcg caaaatcgaa tcgcaactcg aggaggatcg cgccgcgctc
     2101 caagaccctg accttcgtga actagcggag gcggagattc cgtcgctcga agagcaactg
     2161 accgccctcg agcagcgagt cgagctgctt cttttgccgg tggatccaaa cgatcaacgc
     2221 aacaccatcc tggagattcg tggcggggaa ggaggcgaag aagccgcctt atttgccgcc
     2281 gatctctttc gtatgtacgt gcggtttgcg gagtctcgac gctggaaggt agaggtattg
     2341 agtttgagtg aagcttccgc gggggggtac aaggaagtga tcgcattggt cacgggccat
     2401 cacgtctact ctcatttgcg gtacgaagcg ggtgttcatc gggtgcagcg cgtgccggtc
     2461 accgaggcgc aggggcgcat tcatacatcc acggctacgg tcgcggtgtt gcctgaggct
     2521 gaagacgtgg aaatccaaat cgacgacaaa gatcttcgtt ttgatattgc ggcatcgggg
     2581 gggccagggg gacagggggt caacaccacc aatagcgcgg tgcaaatcac ccatttgcct
     2641 acggggttga tcgtcaagtg tcaggacgag cgctcgcaga tcaaaaacaa ggccaaggcg
     2701 atgaagatct tgcggtcccg tttgttggac atggagcaac agaaacggga cgcggaagtc
     2761 tcg
//
LOCUS       DAOSOR010000690         2760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_36641_length_2760_cov_3.624030, whole genome shotgun sequence.
ACCESSION   DAOSOR010000690 DAOSOR010000000
VERSION     DAOSOR010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36641_length_2760_cov_3.624030"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PK710_23670"
     CDS             <1..609
                     /locus_tag="PK710_23670"
                     /inference="COORDINATES: protein motif:HMM:NF021926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HOT12799.1"
                     /translation="RTAWRWCRPPRAAVRVSSACRLLGPLMPVTAVDGALVLRIETQA
                     ALDVLRSQAAGVSGQRLVAVAVDLRGNLQEPARLMLRGIRGIHESRRAVMVSEEIRVG
                     TRVAFAVIDAASAAADLEHTLRQMRRDLAGGMPRFGFYVDCAGRGSQLHGEEGVDLQM
                     LRRFFPGLPIAGVASAFEIGPGARGAAVHLYSGVFCVVCAPS"
     gene            672..1445
                     /locus_tag="PK710_23675"
     CDS             672..1445
                     /locus_tag="PK710_23675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOT12800.1"
                     /translation="MQSTHQREVRSFGASDIGRFRKVNQDAYLRDDDLGLYVVADGMG
                     GHAAGEVASLEAVDTLYGMIKRGLASMGDKLSSGITEDNARAACRLVEGAVQAATYMV
                     FAMAELDREKSGMGTTISALLVLGNHAVIGQVGDSRVYQVRGGKAVQLTEDHTLIAWE
                     LKQGLITPEQAKHSPHKNIITRAVGNRDYVQVDTSLVELHPADRYLLCSDGLHGYLRT
                     EEIAHLCMSDGQSVVENLIHIANARGGKDNITAVLVEVL"
     gene            1552..2559
                     /locus_tag="PK710_23680"
     CDS             1552..2559
                     /locus_tag="PK710_23680"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOT12801.1"
                     /translation="MTFRRVGVVMGGSSGERNVSLASGKAVVDGLSEKGFDVVPIDLE
                     DRKDWASALKGARVDAVFLALHGRLGEDGCVQGLCEVLGIPYTGSGVLASALAMDKLK
                     AKELFHQHNVPTPAYYTLSVYDDLSDIEQIHGSFGFPVIVKPRSEGSSLGVRCAESLP
                     ELVYAVTEAHKLDDHVLVERRIFGREVNVGVLHGRVLGAIEIAPKKGIYDYEAKYTPG
                     MSEYFMPARLPATRYLGVLKVAEKAVKALGCTGACRVDLIVTEHANEVVLEVNTIPGM
                     TKTSLLPKIAAAAGYSFADLCESIMLSARLHARNQTQTPPESQRLPATGTHTETLAKA
                     V"
BASE COUNT          556 a          800 c          788 g          616 t
ORIGIN      
        1 cgcaccgcct ggcgctggtg caggccgcca cgcgcggcgg tgcgcgtttc ttccgcttgc
       61 cgtctgctcg gcccgctgat gcctgtcacc gccgtcgatg gtgccctggt gttgcgtatc
      121 gaaacccaag cggccttgga cgtattgcgg tctcaggcgg cgggcgtttc ggggcagcgt
      181 ctggtggctg tggcggtgga tttgcgaggt aacctccagg agcctgcacg cttgatgttg
      241 cgtggaatcc gcggcatcca cgaatcgcgt cgggctgtta tggtttcgga agaaatccgt
      301 gtcggcaccc gcgtggcttt tgccgtcatc gatgccgcct ccgcggcggc ggatttggaa
      361 cacaccctac ggcaaatgcg tcgtgacttg gcaggaggaa tgcctcgttt cgggttctac
      421 gtggattgtg cgggccgagg cagccagctt catggcgagg aaggggtcga tcttcagatg
      481 ctgcggcgtt ttttcccagg cttgcccatc gctggagtgg cctccgcgtt cgagatcgga
      541 cctggggccc ggggggccgc tgtccacctg tatagcggcg tgttttgcgt ggtgtgtgcc
      601 ccgagctaat gtccatcggg tatttttact ttgctccttt ctccattccc tcactacgat
      661 gacccaccct catgcagtcg actcatcaac gagaggtccg atccttcggt gcttcggata
      721 ttgggcgttt tcgaaaagtc aatcaagacg cgtatcttcg cgatgacgat ctcggattgt
      781 atgtggtggc cgacggcatg ggaggacatg cggctggcga agtggccagc ctcgaagccg
      841 tcgataccct ttacgggatg atcaaacgag gccttgcgag catgggcgat aagctatctt
      901 ctggtatcac cgaggataac gcgcgcgccg cctgtcgtct ggtcgaaggt gccgtacaag
      961 ccgcgaccta catggttttt gccatggctg aactcgatcg cgagaaatca ggaatgggaa
     1021 ccaccatttc cgcattgctc gtgttgggaa accacgcggt gatcggtcaa gtcggagata
     1081 gtcgcgtgta ccaggtgcga ggcggaaaag ccgtgcaact caccgaagac cacaccctca
     1141 tcgcctggga actcaaacaa ggtctcatca ccccagaaca agccaaacac tcgccgcaca
     1201 aaaatatcat cacccgcgcc gtcggcaatc gcgactacgt acaagtcgat accagcttgg
     1261 tcgagctgca tcccgcggac cgatacttgc tgtgttccga tggattgcac ggctatttgc
     1321 ggaccgagga aatcgctcat ttatgcatgt ccgacggtca atccgtggtg gaaaacttga
     1381 tccacatcgc caacgcccgc ggtggcaaag acaatatcac cgccgttttg gtcgaagtgt
     1441 tgtgacgccg atttgttgac catttcgtgt cgtgctgata gcgacaagtc cgatgaaaca
     1501 tggatggatg gccacgaatg tccgtcgcac atccaaccga taggagtacc aatgaccttt
     1561 cgacgagtcg gcgtggtgat gggaggttcc tccggagaac gaaatgtctc gctcgcaagc
     1621 ggaaaagccg tcgtcgacgg gctatccgaa aagggattcg atgtggtgcc catcgatctc
     1681 gaggatcgga aagactgggc cagcgcgctg aagggtgccc gtgtcgatgc tgtttttctt
     1741 gctctccatg gtcgcctggg agaggacggc tgcgttcaag gattatgcga ggtgttggga
     1801 atcccgtata ccggctccgg tgtgttggcc tcggccctcg ccatggataa actcaaagcc
     1861 aaagagctat tccaccagca taatgtccct acgccagcgt actacacgct gtccgtgtac
     1921 gatgaccttt cggatatcga acaaatccat ggttctttcg gttttccggt gattgtcaag
     1981 ccgcgctccg aaggctcgag ccttggtgtg cgttgcgccg aatccctccc cgaactcgta
     2041 tatgcagtca ccgaggcgca taaactcgac gatcacgtgc ttgtggaacg acggattttc
     2101 ggacgagaag tcaatgtggg agtgctgcac ggacgagttc tcggagccat cgagattgct
     2161 cccaaaaaag gcatttatga ctatgaggcc aagtacacgc ccggcatgtc ggagtacttc
     2221 atgcccgcgc ggctccctgc cacccgctac cttggcgtgt tgaaagtcgc cgaaaaagcg
     2281 gtaaaagcac ttggatgcac cggtgcttgc cgtgtcgacc tcatcgtgac ggaacacgcc
     2341 aatgaagtcg tgctcgaagt caacacgatt ccgggcatga ccaaaacgag tcttcttccc
     2401 aaaattgccg cggccgctgg ctatagcttt gcggatcttt gcgaatccat catgctttcc
     2461 gctcgtctgc acgcgcgaaa tcaaacccag accccgcccg aatcccaacg gctacccgcg
     2521 accgggaccc ataccgaaac cctcgccaaa gcggtttgac ttcatctttc cgttccccct
     2581 tttttctcat ccgccactaa gatccccacg ctttgcctga accagccctg caactcgaac
     2641 acctgtcccg acgtttcggt gcggtactcg ctgtctctga cgcttccgcc acgtttcatt
     2701 acggaaagat gcacgccctg gtcggcgaaa atggagctgg aaagtccacg ttgctcaaaa
//
LOCUS       DAOSOR010000691         2749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_36845_length_2749_cov_4.596140, whole genome shotgun sequence.
ACCESSION   DAOSOR010000691 DAOSOR010000000
VERSION     DAOSOR010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36845_length_2749_cov_4.596140"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="PK710_23685"
     CDS             <1..970
                     /locus_tag="PK710_23685"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOT12802.1"
                     /translation="RTLPHISGRYSYNRLPFAYVSSVFSTLAPRKGYLVNDREPVWYE
                     RTIGWTNGLIYAKPRAYEGQNYALSYSIARSGGDLPVGKDLDPYARVGRDPTRGMLGV
                     VRASWTYANTERYAMSVGPERGFSLGAAVDFGDIYTASDYSIYSFTYQTTAYLPMPWL
                     RHHSAAFHLGGGVSAGTYPRRGLYYVGGFMDTNIQDVINNTMFQSGFVLRGYEPISFI
                     GSQYHLANVEYRFPIASVDRGISTLPVFLQRLSGNLFVDYGGAFNTLDTDHPRDQFHT
                     GLGGELLIDLQLGYFGVINSRLGYAKGFGEFAVPGGQTYLAISAPF"
     gene            1127..>2749
                     /locus_tag="PK710_23690"
     CDS             1127..>2749
                     /locus_tag="PK710_23690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12803.1"
                     /translation="MRNWRFSRTTLFAASAIGIIGCVGEEFQDTPAYDAGTKEGAAGG
                     DGSPGGKDSGGFSGESGYAGGSSAGAGGETAGAAGQSGGSAGSAGTAGSAGTAGSAGT
                     AGSAGATGGAAGSGTPCAQGWMSCTTAADCETDVTSHPLHCGTCSHACPSAPFASAVC
                     VSSTCKLSCNNGYDDCDGNPSNGCEVELSSNAKHCGTCGKVCPGGNDAVCVSGVCGLG
                     CTSPRADCDNDPNNGCETNLHESVQHCTKCNQACPTYPHSKPKCSAQGCGFTCEGTWK
                     NCNNIDTDGCEADTMTSLKHCGACNAECGTQNASSQCVSGSCQITCKAGFGDCDKNNA
                     NGCEADFSTNALHCGECGHSCLGSDCSMGYCVPKAGPTFPSPISDFAVSLNDLLVIEQ
                     NVLYRIPFGGTATLSTPATVGGHVVTLPKNSTYTAYYSSEQTEQPVVEQLIKSVTFVQ
                     PPVYSNVAIDTGIQLALAVTADRVYWSTSGYGPNSQRQIRYQSLGSNDGNVLVVPTED
                     AYSIALGSGYLYWPEFNGGRVKRISMQSTTITD"
BASE COUNT          604 a          782 c          757 g          606 t
ORIGIN      
        1 acggaccttg cctcatatca gcggtcgata cagttacaat cggcttccct tcgcctacgt
       61 ctcatcggtc ttcagcacct tggctccccg caaaggatat ctggtcaatg atcgagaacc
      121 ggtgtggtac gaacgaacga ttggatggac caacggtctc atttacgcca agcctcgcgc
      181 gtatgaaggc caaaactatg cgctttctta ttcgattgcg cggtctggcg gtgacctgcc
      241 agtaggcaaa gatctcgatc cttatgctcg ggttggacgc gatccgacac gcggaatgct
      301 cggcgtggtt cgggcatcgt ggacatacgc aaacacggaa cgttatgcga tgagtgtcgg
      361 accagagcgg ggcttcagcc tgggcgcggc ggtcgacttc ggtgatattt ataccgccag
      421 cgattactcg atctattcgt tcacctatca aactaccgcc tacctaccca tgccttggct
      481 caggcaccac agcgctgcgt ttcacttggg gggcggcgta tccgccggca cctacccacg
      541 tcgtggactg tattacgtag gcgggttcat ggacaccaac atccaggacg tcatcaacaa
      601 cacgatgttt caaagcggat tcgtcttgcg tggttatgaa cccattagct tcatcggaag
      661 ccaataccac ctggccaatg tggaataccg cttccccatc gcatcggtgg accgcggtat
      721 ttcgacattg cccgtgttcc tgcaacgact ctccggaaac cttttcgtgg actacggcgg
      781 tgctttcaac acgctcgaca cggaccatcc gcgagatcaa tttcatacgg gccttggggg
      841 cgaattgctc atcgatcttc aactcggcta cttcggcgtc atcaacagtc gtttgggata
      901 cgccaaaggt ttcggcgaat tcgccgtgcc cggtggacaa acctatctcg cgatttctgc
      961 gcccttttga acactcggac agaaaatgta gacataagtg cgcttgcgct tgctaccacg
     1021 gctgattcga tccaagttcg cttctacctt cctgttattt aaccaataaa ttcctgatat
     1081 tataaaatcc cccttcgacc cggggttttt cgtaggagtt ggaaccatgc gcaactggcg
     1141 tttttctcga acaacgcttt tcgctgcctc cgcgataggg attatcggct gtgtcggtga
     1201 ggaatttcag gatactccgg cctacgatgc aggaaccaaa gagggtgccg ccggcggtga
     1261 cggcagccca ggaggcaagg attctggcgg cttttcaggg gaatcaggct acgcgggagg
     1321 ctcttccgcg ggggccgggg gcgaaactgc aggtgctgca ggccaatccg gtggaagcgc
     1381 gggctcggcg ggaacggcag gctcagcggg aacggcaggt tcggcgggaa cggcagggtc
     1441 ggctggcgcc accggtggtg cggcaggatc aggcacgcct tgcgcccagg gctggatgag
     1501 ttgcaccacg gccgcggact gcgaaaccga tgtcacatct catcctcttc attgcggcac
     1561 ctgttctcac gcttgtcctt cggcgccctt cgcatctgcc gtatgtgtct cttccacctg
     1621 caagctatcg tgcaacaacg gatatgacga ctgcgacggc aacccgagca acggttgtga
     1681 agtcgagtta tcgtccaacg ccaagcattg cggaacgtgc ggcaaggtct gccctggggg
     1741 aaatgatgcg gtttgcgtat ccggcgtttg cggtctgggt tgcaccagtc ctcgcgctga
     1801 ttgcgacaac gatccgaaca acggatgtga aacgaacctt catgagagcg tgcagcattg
     1861 tacgaagtgc aaccaagcct gcccgaccta tccgcattct aagccgaaat gctcggccca
     1921 aggatgcggt tttacatgcg aaggaacgtg gaagaactgc aacaacatcg atacggatgg
     1981 gtgcgaagca gacaccatga ccagcttgaa gcattgcggc gcttgtaacg ccgaatgcgg
     2041 aacacaaaac gcgtcatccc aatgcgtttc gggaagctgt cagatcacat gcaaggcggg
     2101 atttggggat tgtgacaaga acaacgcaaa cggttgtgaa gcggattttt ccaccaatgc
     2161 attgcactgc ggggagtgcg gccactcctg cttgggttcc gattgttcga tgggctactg
     2221 cgtgccaaag gccgggccaa cattcccatc gcccatttcc gactttgcgg tgagtcttaa
     2281 cgatctgctc gtgatcgaac aaaacgtgct ttaccgcatt ccctttggcg gaaccgctac
     2341 cctttcgacc ccagcaacgg tggggggaca tgtggtcaca ctccccaaaa actcaacgta
     2401 tacggcttat tactcgtcgg aacaaacaga gcagccagtc gtagagcaat tgatcaagtc
     2461 ggtaaccttc gtacaacctc ccgtctattc caatgtcgcg atcgataccg gcattcaact
     2521 ggccctggcg gttaccgccg atcgcgtgta ctggagcaca tcaggctacg gcccgaactc
     2581 acaacgtcaa atccgctacc aatctctggg ttccaacgat ggaaacgtat tggtcgtgcc
     2641 cacggaagat gcctattcca tcgcgctagg ctcgggctat ctatattggc ccgagttcaa
     2701 cggcggacga gtgaaacgca tctccatgca aagcacaacg atcactgac
//
LOCUS       DAOSOR010000692         2742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_36970_length_2742_cov_4.452921, whole genome shotgun sequence.
ACCESSION   DAOSOR010000692 DAOSOR010000000
VERSION     DAOSOR010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36970_length_2742_cov_4.452921"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PK710_23695"
     CDS             complement(<1..541)
                     /locus_tag="PK710_23695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12804.1"
                     /translation="MVARTTFLSSLGCLGVGLILTTASPAGADPIPRYQANVKGNVRV
                     FGSTLTYDCGSKVLPPPGATASCAGQKEVSDSAGDLYWRDNLANVSVEPKQARTSATL
                     DLPSGSVVTYARLYWSALKVGDQPDKDAVLDWVNGSPTTIVSDHCHPTIPFPFSTHPD
                     WFYYQCSGDATDYVRKLGAG"
     gene            complement(665..1861)
                     /locus_tag="PK710_23700"
     CDS             complement(665..1861)
                     /locus_tag="PK710_23700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12805.1"
                     /translation="MRASRWILPFAGLVLWSLGCSDDDTSTAPHAKVGDDAGTDGSVL
                     LPEAAQESSVVDAGHDVVDEQDAFFDAPVDVAADQEEDAPIVVPKPTPADIDFKALQP
                     IPSGEFLLFNDWNPSPNMVRAISLDGQSVVDVFRAFRIWSLGVSHDSKRIAFACGDPK
                     QAEHYDITLGDAIQHTWIYDVASQSAEVLAFGNLNDECHTFGPDDHNLYVCRRYDFSK
                     TGSFKGWRIGRIDLSSRVFEFLTPEMDSVFALHPQPTPDETEMLYGMIDIPSSPPAGV
                     RSIKKLHFATETFSFVRTDADTPVLSPDGKRYLYRNFLQKRTLWASSLDATNEVQVVA
                     AEVTKPVWSPDGSRVAYLVRDDAHNCDHIEMVASDGSQASAPTRLRDCSTSGEFITNI
                     AWISIP"
     gene            2041..2733
                     /locus_tag="PK710_23705"
     CDS             2041..2733
                     /locus_tag="PK710_23705"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOT12806.1"
                     /translation="MLALSLSKNLLVDLVIYLVPMVLSLSVHEYAHAWMAFRLGDDTA
                     SREGRLTLNPMAHIDVFGTLLIPAFNVLLGGFALIGWAKPVPVRPVRFNRKVTMRVGM
                     ILTAVAGPLSNLMMAVASIGLAALLIRVAPEWLMGDRPTGLAKLLQAMFILNIGLFIF
                     NLLPLPPLDGSRLLPRSMDGFVTHVSPYSFILLLVVINIPFLRETLLVQPVMLTVRLL
                     QTVFSTQLWGVA"
BASE COUNT          627 a          739 c          773 g          603 t
ORIGIN      
        1 cgcccgcgcc caacttgcga acatagtccg tagcatcgcc cgaacactgg taataaaacc
       61 aatctggatg ggtggaaaaa ggaaacggga tggtagggtg acaatgatcc gaaacgatgg
      121 tggtgggcga accattgacc caatcgagca ccgcatcctt gtcaggctgg tcaccgactt
      181 tgagcgcaga ccaatacaga cgcgcgtagg taacgaccga accggaagga agatccaagg
      241 tggccgaagt gcgagcttgt ttgggctcga cagagacgtt ggccagattg tcacgccaat
      301 acaaatcgcc agcgctatcg gaaacttctt tttgtcctgc acacgaggcc gttgctcccg
      361 gaggtgggag gacctttgag ccacagtcat aggtcagcgt ggaaccaaac acgcggacat
      421 tacctttgac attggcttga tagcgaggaa tcgggtcagc gccggcaggc gaggccgtcg
      481 tcaaaatcaa acctacgccg aggcaaccca agctagatag aaaggtggta cgtgcaacca
      541 tagtgggcgc gtatagcacc cctggagccg cggtcgacca caatccgtgg gaatacgggt
      601 ttccgcttct tgaatgtccc aattacacaa tgatcaaaat cgattcacgc gggggggaat
      661 tcacctaagg tattgaaatc caagcgatat ttgtaatgaa ctccccggaa gtcgagcagt
      721 ctcgaaggcg agtcggcgcg gatgcctggg aaccgtccga agccaccatc tcgatgtgat
      781 cacaattgtg cgcatcgtcg cgtaccaaat aggcaacccg cgacccatcc ggtgaccaca
      841 cgggtttcgt tacctccgcc gctaccacct gaacctcgtt cgtggcgtcg agggatgagg
      901 cccataacgt gcgtttttgc aggaaattcc ggtatagata tcgcttgcca tccggggata
      961 acacgggagt gtcggcatcg gttctcacga acgaaaacgt ctccgtcgca aaatgcagtt
     1021 tcttgatgct ccgaacccca gcgggaggcg atgacggaat atcgatcatc ccgtacagca
     1081 tttcggtttc atccggtgtg ggctgggggt gcagggcgaa tacggaatcc atttccggtg
     1141 taaggaattc aaaaacccga ctggataaat cgatacgtcc gatccgccat cctttgaagg
     1201 agccggtctt agaaaaatcg tagcgacggc atacatagag gttgtggtca tcggggccga
     1261 aggtatggca ttcgtcgttg aggttgccga aggccaacac ttcggcggat tgagaagcaa
     1321 cgtcgtagat ccacgtatgc tggatggcat cgccgagggt gatatcgtag tgttcggctt
     1381 gtttagggtc tccacaagcg aacgcgatgc gtttggaatc gtgagacaca cctagactcc
     1441 agattcgaaa cgcgcgaaag acgtccacta cggactgtcc gtccagggat atcgctcgca
     1501 ccatgttcgg tgatggattc cagtcgttga acagcaggaa ttcacctgaa ggaattggtt
     1561 gaagagcctt gaagtcgata tcggcgggag ttggtttcgg aacaacgatg ggggcatctt
     1621 cttcttggtc cgcagcgaca tccacggggg catcgaaaaa cgcgtcttgt tcatccacaa
     1681 cgtcgtggcc cgcatccacc accgaggact cctgagcggc ttccggcaga agcacactcc
     1741 catccgtccc ggcatcatcg cccaccttgg cgtggggtgc ggtggaggtg tcatcgtcac
     1801 tgcaaccaag gctccataga accaggcctg cgaagggaag aatccaacgg gacgcgcgca
     1861 tagagtccag aatacctcaa atggcatggg agagacgatg aacaacccca ttttccgtgg
     1921 aaaacgtcga ggcaaggagg ccaccgtatt ttcgcgacat ggcacgaacg ccgcaccgat
     1981 ctcacgcaaa aaagacgagc ccccttccct cacccgcctt ctcgtgccag tgatccggcc
     2041 gtgctcgctc tgtctctcag taaaaatctt ctcgtcgatc tggtcatcta cctggttccg
     2101 atggttttat ccctgagtgt ccacgaatac gcacatgcgt ggatggcctt tcggctaggg
     2161 gacgatacgg cctcacgaga aggtcgtctg acgctcaacc cgatggccca tatcgacgta
     2221 ttcgggacgc tattgattcc cgctttcaac gtgcttctcg gaggattcgc gttgattggg
     2281 tgggcgaagc ccgttcctgt acgaccggtt cgattcaacc gaaaggtcac gatgcgtgta
     2341 ggcatgatac tgacggcagt agcgggaccg ttatccaatc tcatgatggc ggtggcttcg
     2401 atcggactgg ctgcacttct gattcgagtc gcgcccgaat ggctgatggg agaccgtcct
     2461 acgggattgg ccaagctgct gcaagccatg ttcatcctca acatcggcct gttcatcttc
     2521 aatttgcttc cccttccccc tctggatggt tcgcggcttt tgcctcgttc catggatggg
     2581 tttgtcaccc acgtatctcc ctattcgttc atcttactat tggtcgtgat caatattccg
     2641 tttttgcgag aaacgttgct cgttcaaccg gtgatgttga cggtgcggtt gcttcaaacc
     2701 gtattcagca cacagttgtg gggggttgcg tgactgaggc gg
//
LOCUS       DAOSOR010000693         2740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_37005_length_2740_cov_4.976164, whole genome shotgun sequence.
ACCESSION   DAOSOR010000693 DAOSOR010000000
VERSION     DAOSOR010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37005_length_2740_cov_4.976164"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PK710_23710"
     CDS             complement(<1..481)
                     /locus_tag="PK710_23710"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOT12807.1"
                     /translation="MQRNDPKAHRALNHLAKALFSSLLLGLVLWKVPVQTAWHHLRDL
                     SLTALISIVFLSFAFPAVAAMRWRRVLHHLDAPSTWSPLLLDHLVASTYNMLLPTSIG
                     GDVVRAWRCGRRIDNPAHAWSTVLFERLVGIIALALLSVPGLMMAPGAMRTLGWVVAA
                     "
     gene            complement(521..1315)
                     /locus_tag="PK710_23715"
     CDS             complement(521..1315)
                     /locus_tag="PK710_23715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT12808.1"
                     /translation="MIVDRALEVIRDRSHRQRMADADYWDERVQSRDGDARSVWHSAS
                     FNHAWNHRQMQVLESAFRQLLGDIRGQELLDVGCGTGRITLELQRMGARAVGVDFSSA
                     AVQAASKESVAKEPPIRFMVGDIARPPLPFDNESFDATVAVGCLAVACKDLASLEQAL
                     RELARLTKKSGAIVLLEPIHSSRLLGRVLRASVADWLQAADRAGLQLVARRGMGFIPI
                     RLAMSSFDLPRWMVKPVFRAGENALESLPLQRLADYSLLGFRHAYR"
     gene            complement(1312..2532)
                     /locus_tag="PK710_23720"
     CDS             complement(1312..2532)
                     /locus_tag="PK710_23720"
                     /inference="COORDINATES: protein motif:HMM:NF024925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOT12809.1"
                     /translation="MSKGRHVLIVGCGFPQLGLIRAARRLGIVTTGIDLNPGAVGAAE
                     VDHFLHASTGDSAAICAAFRESKAEGIATSGSELALTTTAHAAERLGLPFYADTETVH
                     RCQAKDAMRACYRAAGLEVPFFESCTEIEQAFDFIERIGLPVVVKPANGWGQRGVCRV
                     DSREQLEYAFHKALKYSHRGGGVVLETCISGSEVSVNGWVENGELVSYCVTDREVFAG
                     HDPLGVMRSEITPSSLDAALINKAVLVAQQGAKALGLLRGPCYSQVAVSQERAVLFET
                     AARLGGGFDADVTRLACGVDLYARLLGIALQDEALESSGSAHALKPALVRFLAPRPGF
                     LQAIEGIERARNVAGVEDLAIYARAGDFLDPLQFAAKRIGHILVTGSSRQQAIDRADE
                     AERHIRLIVQGNKS"
     gene            complement(2519..>2740)
                     /locus_tag="PK710_23725"
     CDS             complement(2519..>2740)
                     /locus_tag="PK710_23725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12810.1"
                     /translation="IWGTQMGWSSIFAAVMAMGGMQLLALSLIGEYVARIFVQTQRRP
                     IFVVAEAMGGANAVEKASTVSKEGEHVEG"
BASE COUNT          599 a          825 c          778 g          538 t
ORIGIN      
        1 cagcggctac cacccagcca agcgttcgca tggcccccgg cgccatcatc agcccgggaa
       61 ccgagagcaa cgccagggca atgattccca ccaatcgttc gaataggacg gtggaccatg
      121 catgagcggg attgtcgatc cgacggccgc accgccaagc gcgcaccaca tcgcctccaa
      181 tgctcgtggg aagaagcatg ttgtaggtcg atgcgaccag gtgatcgagc aacagggggc
      241 tccatgtcga aggggcgtcc agatgatgca gcacgcgacg ccaccgcatc gctgccaccg
      301 cggggaaggc aaaggacaga aaaacgatgg aaatcaaggc agttagcgac agatccctca
      361 gatggtgcca cgcggtttgg acagggacct tccacaacac caaccccaat agaagtgatg
      421 aaaacagagc tttggcgaga tggttcaggg cacgatgagc tttggggtcg tttctctgca
      481 tggggccctt ttttttccgc gcgatcatct cccttgtccg ctagcggtat gcgtgacgga
      541 aaccgagcaa ggaataatcg gcaagacgct gcaagggcaa ggattcgagg gcgttttctc
      601 ccgctcgaaa cacgggtttt accatccatc ggggcaagtc aaagctcgac atcgctaacc
      661 ggatcgggat aaatcccatt cctcttcgag ccacgagttg caatccggcc cgatcggcgg
      721 cttgcagcca atcggccacg gaagcccgga gcacgcgacc gaggagccgt gaggaatgaa
      781 tcggctcgag caatacgatc gcgcctgatt ttttcgtcag tcgcgccagt tcccgcagcg
      841 cttgttccaa tgatgcaagg tccttgcagg ccacggcaag acagccgacc gccacggtgg
      901 cgtcaaacga ttcattgtca aaaggcagcg gcggacgggc gatgtccccg accatgaacc
      961 gaatgggcgg ttctttcgct acggattctt tcgatgcggc ttgcacggcg gcggaagaaa
     1021 aatcgactcc cacggctctg gcgcccattc gctggagttc caaggtgatt cttccggttc
     1081 cacatcctac gtccaacaat tcttgtcctc ggatatcgcc gaggagttgt cgaaatgcgc
     1141 tttcgagcac ttgcatctgg cgatgattcc aagcgtggtt gaacgaagcg gaatgccaca
     1201 cggagcgcgc atccccgtct cggctctgta cccgctcatc ccaataatcg gcatcggcca
     1261 tgcgttgccg atgggaacgg tcgcggatca cttcgagggc gcggtctacg atcatgattt
     1321 gtttccttgt acgatgaggc ggatatgtcg ctcggcctcg tcggcgcgat cgatggcttg
     1381 ttgtcgcgac gagccggtta cgaggatgtg cccaatgcgt ttggccgcga attgtagagg
     1441 atcgagaaag tcgccggcgc gagcatagat ggccaagtct tccactcccg ccacgtttcg
     1501 tgctcgttcg atcccttcga tcgcctgaag aaagcctggg cgaggggcca aaaatcggac
     1561 caacgcgggt ttgagggcat gagccgagcc ggaagactcg agggcctcgt cttgcaatgc
     1621 gattccaagc aatcgagcat ataggtccac gccacaggca agacgggtga cgtcggcgtc
     1681 gaagccacct cccaatcgtg cggcggtttc gaaaagcacc gcgcgttctt gggatacggc
     1741 gacctgggaa tagcaaggtc cccgcaggag accgagagcc ttggcgcctt gttgcgctac
     1801 cagaacggct ttgttgatga gggcagcatc gagcgaggaa ggcgtgatct cgcttcgcat
     1861 gacacccagc ggatcgtggc cggcaaaaac ctcccgatcg gtaacgcaat aggaaacgag
     1921 ttctccgttt tccacccatc catttacgga tacttcgctt ccagaaatac aggtttctag
     1981 aactacgcct ccgcctcgat gagagtattt caaagcttta tgaaacgcat attcgagttg
     2041 ttcccgcgaa tccacccgac agacgcctct ctgtccccat ccgtttgctg gcttgacgac
     2101 aaccggaagg ccgatgcgtt cgatgaagtc gaatgcctgt tcgatttctg tacacgattc
     2161 gaaaaacggg acttccaggc ctgccgcgcg ataacacgct cgcatcgcgt ctttggcttg
     2221 gcatcgatgc accgtctcgg tatccgcgta aaacggcaaa ccgaggcgtt ccgcagcgtg
     2281 cgcggtggtg gtcagggcca gttccgatcc ggacgtggcg atcccctcgg ccttggattc
     2341 tcgaaaagcc gcgcaaatcg ccgcactatc gcccgtggag gcatgaagaa aatggtcgac
     2401 ttcggctgcg cccaccgctc ctgggttcaa atcgatcccc gtggtgacga tgccaagccg
     2461 ccgcgccgcc ctgatcaaac caagctgcgg gaacccgcat cccacgatca atacgtgtct
     2521 acccttcgac atgctcgcct tccttgctga ccgtcgaagc cttttcaacc gcgttcgcgc
     2581 cgcccatcgc ttccgccacc acgaagatgg gtcgacgctg ggtctgcacg aagatccgtg
     2641 cgacatattc tccaatgaga ctcaacgcca aaagctgcat gcctcccatc gccatgacgg
     2701 cggcaaaaat ggaggaccat cccatctgtg tcccccaaat
//
LOCUS       DAOSOR010000694         2726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_37252_length_2726_cov_3.360165, whole genome shotgun sequence.
ACCESSION   DAOSOR010000694 DAOSOR010000000
VERSION     DAOSOR010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37252_length_2726_cov_3.360165"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PK710_23730"
     CDS             complement(<1..257)
                     /locus_tag="PK710_23730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOT12811.1"
                     /translation="MSSPSGAGKTTLTAKLRERFAGLRFSVSHTTRSPRSNEQDGREY
                     HFVDRQRFEAMIAEDAFVEWAEVHDHLYGTSVQEIELARTA"
     gene            complement(301..1164)
                     /locus_tag="PK710_23735"
     CDS             complement(301..1164)
                     /locus_tag="PK710_23735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOT12812.1"
                     /translation="MTGFGIGHAPIGRSRLIVEARSVNHRFLDVRVRACKELIDHVMY
                     VEQLARQQLRRGRVEIALRTDANGGQGFAIDMGRAQAIYESLCALRDRIAPTDDVPFS
                     LLASIPDALVADSAWDAEACQQAISQALLQALQAADDMRKQEGDLLTRDLLTRLETVR
                     NRTQSIAARRCEYSTIVRDRLLGRVQQFISDHDLAIDPSRIAQEVILIVERSDIEEEL
                     IRLESHFQQFEALACTDEPVGRRLDFLLQEMGREINTVGSKSQDACISHAVVDIKAEI
                     ERMREQVQNVE"
     gene            complement(1216..1530)
                     /locus_tag="PK710_23740"
     CDS             complement(1216..1530)
                     /locus_tag="PK710_23740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12813.1"
                     /translation="MWVPKVEEKHYYCHRCRNEIEMIAKLQRTDTCPHCGADLHCCKN
                     CEHWDPSAHNQCKEHIAEYIPDREKTNFCTFFTFRDGKPEDTSRVIASSKAKLDDLFK
                     KK"
     gene            complement(1554..2063)
                     /locus_tag="PK710_23745"
     CDS             complement(1554..2063)
                     /locus_tag="PK710_23745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HOT12814.1"
                     /translation="MRLYKGRIPPIASAIVKALVANNDIETQSPKQVEHDIEAVLKTY
                     LEAERQVNEEARDLMQSRKLAPHELGRIKRLVAERKGIAIGDDTLDYLLDQIVAVLMY
                     SEAVEEVFVEDVDLRRRMAPIFKQEMAVEEQLERETRAQLGHVQEGTRTWEVEYRRVM
                     EEIRRRKGL"
     gene            2181..>2726
                     /locus_tag="PK710_23750"
     CDS             2181..>2726
                     /locus_tag="PK710_23750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12815.1"
                     /translation="MPNRSVLGNWRQLRLLFETMSICPWCKGNTPRAYDTCPLCGKNP
                     HEHPSVSGGNFSSFDSFDDDDAGGLDVQGAIAPLSHADGPDSFGSHALDDAPQGVSLE
                     LDAPPLPSRRPLTPKSLSLSQQPSMPSPAVGSAPPAFDTFDLMILADFGPAPQKITEF
                     PLYAIRVTSRRRELRKQAQLAR"
BASE COUNT          567 a          811 c          695 g          653 t
ORIGIN      
        1 ttggccgtcc gcgccaactc gatctcctga acactcgtgc cgtataaatg atcatgaacc
       61 tccgcccact ccacgaaggc atcctcggca atcatcgcct caaaccgctg gcgatccacg
      121 aaatggtatt cacgaccgtc ttgctcgttg gaccgcggtg accgcgtcgt atgcgaaacc
      181 gaaaaccgta gccccgcgaa tcgctcccgc agcttggctg taagcgtggt ttttcccgct
      241 ccagagggcg aggacaagat cagcaggata aactcgccgc tattaggtaa tgcattgaaa
      301 ttattcaaca ttttgcacct gctcgcgcat gcgctcgatc tccgccttga tatcgacaac
      361 cgcgtgggaa atacaagcgt cctggctttt cgaccccacc gtgttgattt ctcgccccat
      421 ttcttgaagc aggaaatcga gacgacgacc gacgggttca tcggtgcatg ccaaagcttc
      481 gaattgctga aaatggcttt ccaagcgaat gagttcttct tcgatatcgc ttcgttcgac
      541 gatgaggatg acttcctgcg cgatgcgcga tgggtcaatg gctaggtcgt gatcgctgat
      601 gaattgttgc acgcggccca ggagccggtc ccgaacgatg gtcgaatact cgcatcgacg
      661 cgctgctatc gattgggtgc ggttgcggac tgtttcgaga cgcgtcagca agtcgcgtgt
      721 gagaaggtct ccttcttgct tgcgcatgtc atcggcggct tgcaaagctt ggagcagcgc
      781 ttgagagata gcttgctgac aagcctcggc atcccacgcg ctatcagcga ccagcgcatc
      841 cggtatgctg gcgagcaacg agaatggaac gtcgtcggtg ggcgcgatcc gatcgcgaag
      901 cgcacataat gactcgtata tcgcctgtgc tcgccccatg tcgatcgcga atccttggcc
      961 gccattcgca tcggtccgca gggcaatctc gactcttcca cgacgaagct gctgtcgcgc
     1021 cagttgctcg acatacatca cgtggtcgat aagttctttg cacgcacgca cccgcacgtc
     1081 caaaaaacga tggttcacgg aacgagcctc gacgatcaaa cgactccgac cgataggagc
     1141 atgcccgatg ccgaagcctg tcatgctctt caagtcgata cctcgaatcc ctcgattgga
     1201 atcatgtccg tcgacctact tctttttgaa tagatcgtcg agcttggcct tggaactggc
     1261 aatcaccctc gaagtatctt cgggtttgcc gtcgcggaac gtgaaaaacg tacagaaatt
     1321 cgttttttct cggtcgggga tatactccgc gatgtgctcc ttgcattggt tatgagccga
     1381 tggatcccaa tgctcgcaat tcttacaaca atgcaaatcc gccccacaat gcggacaggt
     1441 atccgtgcgc tgcaacttgg cgatcatctc gatctcgttg cggcaacgat gacagtaata
     1501 atgcttttcc tcaaccttcg gaacccacat ctcaagtcct ctcgtgcccg ggcttacaag
     1561 ccctttcgcc gtcgtatctc ttccatcacc cgacgatatt ccacttccca ggttcgtgtc
     1621 ccctcttgta catgccctag ctgcgcgcgg gtctctcgct ccaattgctc ctccaccgcc
     1681 atttcttgtt tgaaaatcgg agccattcga cgacgcaggt ccacgtcttc gacgaagact
     1741 tcttccacgg cttcggagta catgagaacc gcaacgatct gatcgagcag atagtcaagc
     1801 gtatcatcgc caatggcgat gccttttctt tctgccacaa gacgtttgat gcgtcccaac
     1861 tcatgggggg cgagctttct cgattgcatc aaatcccgcg cttcttcatt gacttggcgc
     1921 tcggcttcca aatacgtctt gagcacggcc tcgatatcat gctccacctg tttgggggat
     1981 tgcgtttcga tgtcgttgtt cgcgacgaga gccttgacga tggccgaagc gatggggggg
     2041 attcttccct tgtaaaggcg catggggaac tcgcaggaag gtgaatattc agatcctatt
     2101 cctcacccta gaaggcatgg tcccagggtg tcaatcgaag gtgcccgcga cggcgcgcaa
     2161 ccttgaccat tgtgtttccc atgccgaatc ggtccgtgct tgggaattgg cggcaacttc
     2221 gtttattatt cgaaacaatg tccatctgcc cctggtgcaa aggaaacaca ccgcgtgcct
     2281 atgacacttg ccccctgtgc ggaaaaaatc cgcacgagca tccgtcggtt tccggaggaa
     2341 acttctcttc tttcgattct ttcgacgatg acgatgcggg ggggctggat gtgcaagggg
     2401 ccatcgctcc gctgagccat gccgacggac cggactcttt tggctcccac gcgctcgatg
     2461 atgcccctca aggcgtttct ctcgaactcg atgccccccc gctgccttcg cgccgtccgc
     2521 tgacgccgaa atccctgtcg ctttcgcaac agccttctat gcccagtcct gccgtcggtt
     2581 cggccccccc cgcgttcgat acctttgatt tgatgatact cgcggatttt gggccagctc
     2641 cacaaaaaat aacggaattt ccactatatg caattcgtgt gacgagccgt cgacgagaac
     2701 tgcgaaaaca agcgcagttg gcaagg
//
LOCUS       DAOSOR010000695         2716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_37435_length_2716_cov_4.973318, whole genome shotgun sequence.
ACCESSION   DAOSOR010000695 DAOSOR010000000
VERSION     DAOSOR010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37435_length_2716_cov_4.973318"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="PK710_23755"
     CDS             <1..958
                     /locus_tag="PK710_23755"
                     /inference="COORDINATES: protein motif:HMM:NF021759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HOT12816.1"
                     /translation="LTDTSSILGRWHSETRTIEINRAFAIHQPWVHVIEVLKHEMAHQ
                     FVDQILGQKNDGHGELFRSVCQRFCIDPRASGLPNAHPPSEQEERVLSRVARLLALAD
                     SPNTHEAHAAMSAAQRLMLRYNIDQARLASGQSRYEFRQVGHITGRIQESERILAALL
                     IEHFFVNALWVQAYVPMTGKSGSVLELCGTPANLEMAEYVYAFLSHTAQQLWNAHQKS
                     TKCSGRDRQTYLAGVMLGFRERLARESTAQQCEGLVWAGDPGLDAYLRARHPHTRRLV
                     RYGNRRTQAREHGKRAGREIVLRRPFEAQPTNDGRLLPSKSR"
     gene            complement(950..1363)
                     /locus_tag="PK710_23760"
     CDS             complement(950..1363)
                     /locus_tag="PK710_23760"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOT12817.1"
                     /translation="MLTWDDRFSVKVTVIDEQHKQLFGSARRFSEAMQRNESREAFAE
                     LLGFLIEYGKMHFQTEEAIFLQCGYEAAASHIEQHQLFLDIVQGFKLRFESDDLIVAA
                     EVVHFLENWLVEHVTTSDQRYVPCLAAQGLTKPSS"
     gene            1518..2288
                     /locus_tag="PK710_23765"
     CDS             1518..2288
                     /locus_tag="PK710_23765"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOT12818.1"
                     /translation="MLLAIDIGNTNISFGVFDTTDLIHHFRAESSRTRTIDEYCVFVE
                     QMLALLNVERRALNAAIIASVVPTLTEPMSSLARRMFACEPVVVGPGTRTGMPILYDN
                     PRDVGADRIVNAVAAYDIVRAGVVVVDFGTATTFDCVSPKGEYLGGAIVPGIQISAEA
                     LFARAAKLPRVDLTAPAKVIGRNTPHAMQSGITYGYASLVEGMIERIRNEVGFPCAIL
                     ATGGLAPLIAKLTSCIEHVDDSLALRGMRIVYERNRPT"
     gene            2302..>2716
                     /locus_tag="PK710_23770"
     CDS             2302..>2716
                     /locus_tag="PK710_23770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOT12819.1"
                     /translation="MRKFMLSVGIAALLLMLVTWLAAALGTESVSFQRAMTDAASIDH
                     RILFDVRLPRIALSAVAGAGLAAVGAAFQALLRNPLAEPYVLGVSGGAALGATLVIAF
                     GVPLAAVWSSALIPASAMIGGLAATALVYAIARAGT"
BASE COUNT          594 a          808 c          726 g          588 t
ORIGIN      
        1 tttgaccgac acctcgtcga tcctcggaag atggcactcc gaaacacgca ccatcgagat
       61 caatcgcgcc tttgccattc atcaaccttg ggtacacgtg atcgaagtgc tcaaacatga
      121 gatggctcac caatttgtcg atcagatcct gggacaaaaa aacgacggac acggagagct
      181 ctttcgctcg gtttgccaac gtttctgcat cgatccgcga gcctcgggct tgcccaacgc
      241 ccacccaccc tcagaacaag aagaacgcgt tttgtctcgt gtagcccgtc tgttggccct
      301 cgccgacagc cccaacaccc acgaagccca cgcggccatg agcgccgccc aacgcttgat
      361 gcttcgatac aacatcgatc aagcacgtct cgcttcaggc caatcgcgat acgagtttcg
      421 acaagtagga cacatcaccg ggcgcatcca agaaagcgaa agaatcctcg ccgcattgct
      481 catcgaacac ttctttgtca atgctctctg ggttcaagct tacgtgccga tgacaggaaa
      541 atcaggcagt gtgctcgaac tatgcggaac gcccgccaac ctcgaaatgg ctgaatacgt
      601 gtacgccttc ctgtcgcata ccgctcagca attatggaat gcccatcaga agtcgacaaa
      661 atgctccggc cgagaccggc aaacgtatct cgctggtgtg atgctaggat ttcgagaacg
      721 cctggcgcga gaaagcacgg cacagcaatg cgaaggactc gtttgggccg gcgatccggg
      781 tctcgacgcc tatctgcgag cgcgacatcc ccatacgcga cgtttggttc gctacggcaa
      841 tcgacgaacc caagcgagag aacacggcaa acgcgcgggt cgcgagatcg tccttcgacg
      901 ccccttcgag gcgcagccaa cgaacgacgg acggcttcta ccgtcgaagt caagatgatg
      961 gtttcgtcaa tccttgcgcc gccagacacg gaacgtatct ctgatctgag gtggtcacat
     1021 gttcgacgag ccaattttct agaaaatgca cgacttcggc ggccacgatg agatcatcgc
     1081 tttcgaaacg gagtttgaaa ccttgcacga tatcgaggaa aagctggtgc tgctcgatat
     1141 gggatgcggc cgcttcgtac ccacactgca agaagatcgc ttcctccgtt tggaagtgca
     1201 tctttccgta ttcaatcaaa aacccaagca gctctgcaaa agcctcccga ctctcgttac
     1261 gctgcatggc ctcggagaaa cgtcgagcag atccaaatag ctgcttatgc tgctcatcga
     1321 tgaccgtaac cttgacgctg aatcgatcgt cccaagtgag catgatggcc tagcgtagcg
     1381 tgacacacgt ccaatgcaat actgctagat caatacacac ggcacccaga cgttccttgg
     1441 agactccgtg tacgctttcg tcaatcacca cgactcgctg ttggtgagcg gattgttcat
     1501 gagatagtct tgccccgatg ctgctcgcaa tcgacattgg caacaccaac atcagttttg
     1561 gtgtatttga cacaacggat ctcattcatc acttccgtgc cgaaagctca cgcaccagga
     1621 cgatcgatga gtactgcgtg ttcgtagaac aaatgctcgc gttgctgaac gtggagcgtc
     1681 gggccttgaa cgccgccatc atcgcaagcg tcgtacccac gttgaccgaa ccgatgagtt
     1741 cccttgcgcg acgaatgttt gcgtgtgagc cggtcgtggt gggacctggc acacgcacgg
     1801 gaatgcccat tctgtacgat aatccgagag acgtgggcgc tgaccgcatc gtcaatgcgg
     1861 tcgccgcgta cgatatcgtt cgtgccggcg tggtggtcgt cgattttggc accgcaacga
     1921 catttgattg tgtgagcccg aaaggagaat atttgggagg cgccatcgta ccgggaattc
     1981 agatcagcgc ggaagctctg tttgccagag ccgcaaaact cccccgcgtc gacttgacag
     2041 ctcctgccaa agtcattgga cgcaacaccc ctcatgccat gcaatcaggc atcacctatg
     2101 ggtatgcgtc gctcgtcgag ggtatgattg aacgtattcg caatgaagta gggtttcctt
     2161 gtgccatcct ggccaccgga gggctggcac cactcattgc caagctcacc tcatgcatcg
     2221 agcatgtgga tgatagtctg gcgcttcgag gcatgcgtat cgtgtacgag cgcaatcgac
     2281 ccacctagac aagaggaatc gatgcgcaaa ttcatgctct ccgttggcat cgctgctttg
     2341 ctgttgatgc tcgtgacctg gctggcagcg gccctcggca cggaatccgt gtctttccag
     2401 cgggcgatga cggatgcagc aagcattgat caccgcattt tattcgatgt tcggttaccc
     2461 cgaatcgcgt tgtcggccgt cgcgggggcg ggccttgctg ccgtgggtgc cgcgtttcaa
     2521 gcgctgcttc ggaatcccct tgccgagcct tacgtactgg gagtctccgg tggcgctgcg
     2581 ctcggcgcca cgctcgtcat tgctttcgga gttcccttag ccgccgtatg gtcgtcggcc
     2641 ttgattcccg catccgccat gataggcggc ttggcggcca ccgccctcgt gtatgccatc
     2701 gcccgcgccg gcaccg
//
LOCUS       DAOSOR010000696         2708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_37572_length_2708_cov_4.637769, whole genome shotgun sequence.
ACCESSION   DAOSOR010000696 DAOSOR010000000
VERSION     DAOSOR010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37572_length_2708_cov_4.637769"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PK710_23775"
     CDS             <1..816
                     /locus_tag="PK710_23775"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOT12820.1"
                     /translation="LGTDLAAEAIDVAKKAIYPGSATMDMPVAWREQFFEKEEAGWFR
                     VKDSVRRLVRFDVHNLAHRPPTGPFHVITCRNVLMYLQQDAQHKAVGALASVLAKDGV
                     LAVGHGESLRGFENLLVPDRGSAVNLYRLAKGIAMASEAMASEAMASEAMASEATPSE
                     LMSLGEKPLAPPLSAPVSRAKPPKNTHTIVLKGVYDEQANPQGVASLKAALADAVAGG
                     LSVIIEADGALSLDGATARLILRAMRTCPMPMVVHATRDSVKQWARRYGVPLG"
     gene            1140..1880
                     /locus_tag="PK710_23780"
     CDS             1140..1880
                     /locus_tag="PK710_23780"
                     /inference="COORDINATES: protein motif:HMM:NF012693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOT12821.1"
                     /translation="MPEAFGLSKTGRRPRNEDSFSTRSDLGVYVVADGMGGHAGGHVA
                     SRLVVDTLERFFDQVGDSDVGMDAESQGVSLAEQRMDLAIRMCKREVERKAVGDLAPM
                     GTTLAALLLRDGRAVLAHVGDSRIYRLRRGHLEQMTRDHSLCAELEAAGGHELVKRLT
                     GPYTAMVTRSISAYSNAKPDIRTERTEAGDRYLLCTDGLSGALDAEAMASILRIYKEP
                     QEAACRLVQLALAAGSMDNVTAVVVDPA"
     gene            1980..>2708
                     /locus_tag="PK710_23785"
     CDS             1980..>2708
                     /locus_tag="PK710_23785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12822.1"
                     /translation="MADTEDFGGFDDELVEIERATAILHQRDPSQAELVVQELKARRE
                     EELRREEVARKIREIAAKRRRVRKKRIAIVAGMVVVGAAAAIPLARAVLQEAARSKAL
                     QAELTQQALPLSSMGFEQQAEWLDVPPVGVVFEVPRNTCSAVLGVAENENQKLPIQVA
                     RPGLEPVSHQGGLVWCSCDKEQVTASVVDPGNKRVALRWLNTKMGNVGGIEVLMSHAT
                     PAFRVVDDPRAYGCADAAFSLWAQS"
BASE COUNT          548 a          615 c          947 g          598 t
ORIGIN      
        1 cttggaacgg atctggccgc cgaagccatc gatgtggcaa aaaaggcgat ttatccaggt
       61 agtgcgacga tggatatgcc agttgcgtgg cgtgaacagt tcttcgaaaa agaggaagcg
      121 ggttggtttc gtgtcaaaga ctccgttcga cggctcgttc gttttgacgt gcataacctt
      181 gcccatcgtc ccccaacggg cccttttcac gtgatcacat gccgaaacgt ccttatgtac
      241 ctgcaacagg atgcccagca taaggccgtt ggcgctttgg cttccgttct tgcgaaagat
      301 ggcgtgcttg cggttgggca tggcgagagc ctgcgagggt tcgaaaatct tctcgtgcct
      361 gaccgaggca gcgccgtgaa cttgtaccga ctcgcgaaag gcatagcgat ggcgtcagaa
      421 gcgatggcgt cagaagcgat ggcgtcagaa gcgatggcgt cagaagcgac accgtcggaa
      481 ctcatgagct tgggcgaaaa gcctctggcc ccgcctttgt ctgctccggt atcgcgagcg
      541 aaacccccaa aaaacaccca tacgatcgtc ttgaaaggcg tttacgacga gcaggccaat
      601 ccgcaggggg tagcgtcgtt gaaggctgct cttgccgatg cggttgctgg ggggctttcg
      661 gtgatcattg aggccgatgg ggcattgagc ctcgatggtg cgaccgcgcg gttgattctt
      721 cgggcgatgc gaacctgtcc gatgccgatg gtggtgcatg cgacgcggga tagtgtgaag
      781 caatgggctc gtcgttatgg cgttccgctt gggtaggcgt tgtgggcaaa ggggcgacga
      841 tagtattggc gcttgcggat ccatgactca cccttaatag tgtaactata ggatattata
      901 gtataaatat cgttttggcc caaagggggg cgctgacaag aaatccggaa cggcggtgat
      961 ctttcggttg catcgaagca gcggtttgga ttttggaggc ttgcagatct gcaaacgtaa
     1021 ccgtgtaagt tgttgataaa agtggagaac cattgggggg cctgggagga aaacgcgaag
     1081 aattggctga atattgagga attttccatc ttggcatgat gatcgctacg cgacaggcta
     1141 tgccggaagc atttggcttg agcaagactg gtcgccgacc tcgcaacgag gattcttttt
     1201 ccacacgttc tgaccttgga gtgtatgtgg tggcggatgg gatggggggg cacgccgggg
     1261 gccatgtggc gagtcggttg gtggtggaca ccttggagcg atttttcgac caggtgggcg
     1321 attcggatgt ggggatggat gcggagagtc aaggggtgtc ccttgcggag cagcgtatgg
     1381 atttggcgat acggatgtgc aaacgggagg tggagcgcaa ggcggtgggg gatttggcgc
     1441 cgatggggac cacgttggcg gcattgttgt tgcgggatgg acgcgcggta ttggctcatg
     1501 tgggggacag tcggatttac cggcttcgtc gggggcatct cgagcagatg acgagggatc
     1561 attcgctttg tgctgaattg gaggcggcgg gaggacatga attggtcaaa aggctgacgg
     1621 gaccttatac ggcgatggtc acccgttcga tttcagctta ttcgaatgcg aaaccggata
     1681 ttcgcacgga gcggacggaa gcaggggatc ggtacttgct ttgtacggat gggttgagtg
     1741 gggcgctcga cgcggaggcg atggcatcga ttcttcggat atacaaggag ccccaggaag
     1801 cggcctgtcg tcttgtgcaa ctggctttgg cagcgggaag catggacaat gtcacggcgg
     1861 tggtggtcga tccggcctga gtgactccaa aaacgcgcaa ggggcttgac ccaaaggttc
     1921 tttccgttgt tttctcacga cggcgctccc gcgtcgggta gggaaggact ctgggattct
     1981 tggcggatac ggaagatttt ggcggctttg acgatgaact cgttgagatc gagcgagcga
     2041 cggccattct ccatcagcgg gacccgagcc aggcggagtt ggtggttcaa gagctgaaag
     2101 cgcgtcggga agaggaactg cgccgagaag aggttgcacg aaaaatccgc gaaattgccg
     2161 caaaacggcg tcgcgtgcgg aaaaaaagaa tcgccattgt ggcgggtatg gtggtggtag
     2221 gggcagcggc ggccattccg ttggctcgcg cggtcttgca agaggccgct cggagcaaag
     2281 ccctccaagc ggagctgacg caacaggcgt tgcccctgtc gtccatgggt tttgagcagc
     2341 aggccgagtg gctcgatgtg cctcccgtgg gtgtggtttt cgaggtgcct cgcaacacgt
     2401 gttcggcggt gttgggggtg gcggaaaacg aaaatcaaaa gcttccgatt caggtggctc
     2461 gtccgggcct cgagccggtt agccatcagg gggggcttgt ttggtgttct tgtgacaagg
     2521 agcaagtgac cgcgagcgtg gtcgatccag gaaacaaacg cgtggctttg cgttggctca
     2581 acaccaagat gggcaacgtg ggagggatcg aggtgttgat gagtcacgcg actccggcgt
     2641 ttcgggttgt cgacgatccg cgcgcttatg gttgcgcgga tgcggctttc tccctgtggg
     2701 cgcagagt
//
LOCUS       DAOSOR010000697         2706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_37625_length_2706_cov_3.344776, whole genome shotgun sequence.
ACCESSION   DAOSOR010000697 DAOSOR010000000
VERSION     DAOSOR010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37625_length_2706_cov_3.344776"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1402
                     /locus_tag="PK710_23790"
     CDS             <1..1402
                     /locus_tag="PK710_23790"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOT12823.1"
                     /translation="VLPFGFIRFFYAPWLEAQLRSQAPKGVPPTTTGHVLFCSWDAIT
                     RGLAEHFSLLGIPYYVLEPDPVKATDLNMEGTPVVRGDLDACATYEAARISQARAVYT
                     GLDDATNTSIILTVREACTTVPMIASAENDESVDLLELAGASHVLPLKFRLGEQLANR
                     VQASECRSHVIGQFENLIIAEFPVYKTPFSGRTLLESEIRKLTGVNVVGVWERGRLRQ
                     VKPTTILEDWTIPVVVGTAEQIAALDEQLHTDSVNGHPVIVVGGGKVGRATAAALVRK
                     AVPVHLLEHDPVVAAKAQPYATQVFVGEGADRSLLLQAGIDKAPSVVLTTNDDAMNIY
                     LSIYFRKLNPDLRIVARITHERNIESIHRAGADFVLSYSALGQESVLAIVQKRDFVFL
                     GGDVEFFVLDVPPSLVGKTLRGSGIGAQTGLNIIGLREEHATTNPGPGTVLVQGAELL
                     AIGTQQQHGEFKKIFG"
     gene            1601..2104
                     /locus_tag="PK710_23795"
     CDS             1601..2104
                     /locus_tag="PK710_23795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12824.1"
                     /translation="MNRKTFLLIGLTALGLSMATPLSQARADEIRKSGNFGLGLGVST
                     FAAPLSAKYFMADSHALQFNAGFFQDDLDFEGQGVGLGLDYLFEMPAITSGGGVELGW
                     AAGLGPAFGTAHGHSALAAVGVLGLEFNIKAISVDFVLEYRPQLLLTPDVDLDLVEFG
                     GHARYYF"
     gene            2257..>2706
                     /locus_tag="PK710_23800"
     CDS             2257..>2706
                     /locus_tag="PK710_23800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12825.1"
                     /translation="MIRARVFPILMTIPSLLVAACSCSEEGGTASPPGADSGTDGAMD
                     GSTDGPMDSTIFDTSKPDVDSECPGGCDDGEVCSHGQCVPLSPCTDDNDCSNDSYCDP
                     QTGCIPWGTPPGKTHDPECIFVIAPGQFAPTVRCEFSSAPPGDPFPNH"
BASE COUNT          541 a          714 c          802 g          649 t
ORIGIN      
        1 cgtgcttccc ttcggtttca ttcgtttttt ttatgcgcct tggctcgagg cgcagcttcg
       61 gtcccaagcg ccgaagggcg tacctccgac cacgacgggg catgtattgt tttgttcgtg
      121 ggatgcgatc acacgggggc ttgcggagca tttttctctg ttgggcatac cgtattatgt
      181 gctggagccg gatcccgtga aggcaacgga tttgaatatg gaggggacac ctgtcgtacg
      241 aggggatctc gatgcgtgtg cgacgtatga agccgcacga atttcgcagg cgcgagcggt
      301 gtatacgggt ttggatgacg ccaccaacac gagcatcatt ctgaccgtac gggaggcgtg
      361 tacgacggtt cccatgattg catcggcgga gaatgatgag tctgtcgatc tattggaact
      421 cgcgggagct tcgcatgtat tgcctttgaa gtttcgattg ggcgaacaat tggcgaaccg
      481 ggtacaagca agcgagtgcc ggtcgcatgt gatcggtcag ttcgaaaacc tcatcattgc
      541 cgaatttccg gtgtacaaaa ccccattttc gggtcgtacg ctgctcgaat ccgaaatacg
      601 caagctcacg ggagtcaacg tcgtgggagt gtgggagcgg gggcgtttgc ggcaagtcaa
      661 accgacgacg attctcgagg attggacgat tcctgtggtg gtggggacgg cggaacagat
      721 tgcggctttg gatgaacagc ttcacacgga ttcggtcaat ggtcatcccg tgatcgtggt
      781 gggtggggga aaagtaggga gagcgacggc ggctgcgttg gttcgaaaag ccgtgcccgt
      841 ccatttgttg gaacacgatc cggtggtggc ggccaaagcg caaccttatg ccacccaagt
      901 tttcgtggga gaaggcgcgg atagaagtct tctgctgcaa gcaggaatcg acaaggcgcc
      961 atcggtggtg ctcaccacca atgacgacgc catgaacatt tatttaagta tctattttcg
     1021 gaagctcaat cccgacttac ggatcgtggc ccgtatcacc cacgagcgta atatcgagtc
     1081 gattcatcgc gcgggggccg atttcgttct cagctattcg gctcttggtc aggaatcggt
     1141 acttgccatc gtccagaagc gggatttcgt gtttctcggt ggggatgtcg agtttttcgt
     1201 gctcgacgtt cctccctccc tggtgggaaa aacgctgcga ggaagcggta ttggggcaca
     1261 aacgggactc aacatcatcg gacttcgaga ggagcatgct acgacgaacc ctggtcctgg
     1321 cacggtgctc gtgcaaggcg cggagttgct tgcgatcggt acgcagcagc aacatgggga
     1381 attcaaaaaa atcttcggat gatagggcga tcatctccgc gcaaaccacc atggttccgc
     1441 caggcaagga gccagcggaa ggatcgagcg atcagggttg catcatcgga gctgcatctt
     1501 tatgtaccta acgtgctcca accttcagcc tcccatggat gcaccgcata ccacttatcg
     1561 ttacctcttg ttccgaggag aaaggaatca ttcctccatc atgaatagaa aaactttctt
     1621 gcttattgga ttgaccgctt tgggtttgag tatggcaacg cctttgtccc aggctcgcgc
     1681 ggatgagatt cgcaaaagtg gcaacttcgg gcttgggctt ggggtaagca cgtttgccgc
     1741 accgctatcg gcaaagtact tcatggccga cagtcacgct ctgcaattca atgcgggatt
     1801 ttttcaagac gatctggatt tcgaggggca aggggttgga ttgggactcg attatctttt
     1861 cgagatgccc gcgatcacca gtggaggtgg cgttgaactc ggatgggcgg caggtctcgg
     1921 tcctgccttc ggaactgctc atggccactc cgcccttgcc gcggtaggag tgctcggtct
     1981 cgagttcaat atcaaagcca tctccgtgga cttcgtattg gagtatcgcc ctcaattgct
     2041 cctcacccct gacgtcgacc tcgatctcgt cgaattcgga gggcatgcgc gctattactt
     2101 ctgatccccg tatccggtag gcacctattt tcttggcgcg tgcccccgcg ggcaggctgg
     2161 acctttgccc ttcggcggcc ccgaagaatc taccggctca tcccttccag ctcgtgtatt
     2221 ctttcatgag cctcccccga tccaggagct gttgccatga ttcgagcccg cgtatttccc
     2281 atcctgatga cgatcccgag tcttctggtt gccgcttgtt cttgcagtga ggaaggaggg
     2341 accgcatcgc ctcccggcgc ggattccgga accgatgggg cgatggatgg gtcgacggac
     2401 ggacctatgg attcgaccat cttcgatacc agcaaacctg atgtcgacag cgagtgtccg
     2461 gggggctgcg atgatgggga agtatgttct cacggccaat gtgtccccct ctcgccctgc
     2521 acggatgaca acgattgcag caatgacagc tattgcgatc cgcaaaccgg ttgcatccct
     2581 tgggggacac cgcctggtaa gacgcacgat ccggagtgca tttttgtaat cgctcctggt
     2641 cagtttgcgc ccacggttcg gtgtgagttt tcctcagcgc cacccggcga tccgtttccg
     2701 aaccat
//
LOCUS       DAOSOR010000698         2705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_37649_length_2705_cov_4.300000, whole genome shotgun sequence.
ACCESSION   DAOSOR010000698 DAOSOR010000000
VERSION     DAOSOR010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37649_length_2705_cov_4.300000"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="PK710_23805"
     CDS             <1..977
                     /locus_tag="PK710_23805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12826.1"
                     /translation="AVVTATPAPVATATPAPAPTATPAPVPAPEPAAAPAPLFGAPSP
                     VASVEPLFGSGVAAVQAKHEKKPDLFSTQMTAAQEEEVATSAPRISPATSKPTGARDE
                     SSVLFSLSALTSAAAASAPKSDANSGGDDSGLIDLRALADKDSAGAQPALAMPTGGLL
                     DAAPLLGTPLVDPSLHQLKASDPPPAKSNKPLIIGIAIGAVGILSAVIAVVVVLTREQ
                     PAPVVMPVATTTEVEQPTTPIPVPTPSDTSSSAAAAPATGGPPSATTTARPIVRSTGP
                     KSTSTSTAPGKTPSGPAEPATPPAKKPPANKCNCPPGDLACNMACAVK"
     gene            complement(970..2067)
                     /gene="mutY"
                     /locus_tag="PK710_23810"
     CDS             complement(970..2067)
                     /gene="mutY"
                     /locus_tag="PK710_23810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOT12827.1"
                     /translation="MNTPLAPHDWHPNTLRAMRHELLTWFEKYGRQLPWRTKPRDPYA
                     VWVSEIMLQQTRVATVVPYFERFMHRFPTLEALACAPVDDVLSAWSGLGYYRRARALH
                     ATARQVMQQQGRLPSDHAHLLRLSGIGYYTAAAIASIAFDIPVAVVDGNVIRVLTRLT
                     NDASSMDSTTARNDIHQLANRLIDPKRPGAYNEAIMELGALLCTPRTPSCAHCPWRKR
                     CLGLHAQTADQLPVLAKRKPSALLALEAFVITDNQQRVLLCRRHPRGLYGGLWEPPMS
                     AEGPQATRMVESWIGKKGQRLQPRLTHVLTHRVLSIRVTRWALDSAEAPKNLLFTNTY
                     DRMVWLSETAMAERGISSLAMKVLAFSRRAT"
     gene            complement(2064..>2705)
                     /locus_tag="PK710_23815"
     CDS             complement(2064..>2705)
                     /locus_tag="PK710_23815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12828.1"
                     /translation="PMLDAQLAARAHVAPCFVMAFFSAASCQLPTSAPDAFEAGQQDA
                     TLPLVSASPASTQTPFDAAPMAVANVDHPFHGPAWTACTNGFRTSNQPQRDVTRLSLS
                     CAPYQGMRRFGRTFFHRATPSSPGSFSIRLATAQCARVFVTADNQVHSFRVSILDSQE
                     AIVGYAHSRSSPFAVANESGAFCVPRTGEYRIRIDIEQGSGLIAAEVWTLPPP"
BASE COUNT          549 a          779 c          865 g          512 t
ORIGIN      
        1 ctgccgtagt caccgcaaca cctgcccctg tggcaacggc aacgccggct cccgcgccaa
       61 cggcaacgcc cgctcctgtg cctgcgcccg aacctgctgc cgcacccgcg ccattgttcg
      121 gagcgccttc tcccgtcgct tctgtcgaac cgctttttgg ctctggggtt gccgcggtcc
      181 aggcgaaaca cgaaaagaaa cccgatctat tcagcacgca aatgaccgct gcgcaggagg
      241 aagaagtggc cacgagcgcg ccacgaatca gccccgcgac cagcaaaccc actggcgcgc
      301 gagatgaatc ttccgttctg ttttctctga gcgcattgac ttccgccgcc gctgccagcg
      361 ctcccaaatc cgatgcgaac tccggaggag atgactccgg tttgatcgac ctgcgcgcgc
      421 ttgcggataa agacagcgca ggggctcaac ccgctctggc catgcccact ggcggactgc
      481 tcgatgccgc acctcttctc ggaacacccc tcgttgaccc gagtttgcat caactcaaag
      541 cttccgaccc gccacccgcc aaaagcaaca aacctctcat catcggtatt gccatcggag
      601 ccgtgggtat cctgtccgcc gtcatcgccg tggtcgtcgt gctcaccaga gagcagcccg
      661 ctcccgtagt catgccggtc gccaccacca cggaagtcga acagccaacc actcccatcc
      721 ccgtgcctac gccatcggat acgagttcat cggcggctgc ggcgccagcc acgggaggtc
      781 cgccttccgc aaccacgacc gctcgaccca tcgttcgctc gacgggaccg aaatcgactt
      841 ctacgagtac cgccccggga aaaactccgt caggacccgc cgaaccggcc actccgccag
      901 ccaaaaaacc accggcaaac aaatgcaact gtccgccggg ggatctcgct tgcaacatgg
      961 cttgtgccgt caagtagcgc gtctggaaaa agcaagcact ttcatcgcca gcgaggatat
     1021 ccctcgctcg gccattgcag tctcgctcaa ccagaccata cggtcataag tatttgtaaa
     1081 taataagttt tttggtgctt cggcagagtc cagggcccag cgggtcacgc gaatggaaag
     1141 cacccgatgg gtcaagacat gcgtgaggcg cggctgaagc cgctgtcctt tttttccaat
     1201 ccacgattcg accatgcgtg tcgcctgcgg cccctccgcg gacatggggg gttcccacaa
     1261 tcctccatac aatcctcgag ggtggcgacg gcacagcaga acccgctgtt ggttgtcggt
     1321 gatgacgaag gcttcgaggg cgagaagggc agagggtttt cgttttgcga gcacgggaag
     1381 ttgatcggcg gtttgggcat gcaagccgag gcatcgtttt cgccaagggc aatgggcgca
     1441 cgatggtgtt cgtggggtgc agagaagggc tcccagttcc atgatcgctt cgttgtatgc
     1501 accggggcgt ttgggatcga tgagtcggtt ggcgagctga tgaatatcgt tgcgtgcggt
     1561 tgtcgaatcc atgctcgagg catcgttggt gagccttgtg aggacgcgaa tcacgttgcc
     1621 gtcgacgaca gccaccggga tatcgaaagc gatgctcgcg atggcggcag cggtgtagta
     1681 tccgattccg gatagtcgaa ggaggtgagc gtggtcggaa ggcaaccttc cttgttgttg
     1741 catgacttgc ctggccgtag cgtgcagggc tcttgctcga cggtagtagc cgagtccgct
     1801 ccatgcgctg agcacatcgt cgacaggtgc gcaagccagg gcctcgaggg tgggaaaccg
     1861 atgcatgaat cgttcgaagt aaggcacgac ggtagcgacg cgtgtttgtt ggagcatgat
     1921 ttcgctgacc catacggcgt aggggtcgcg aggcttggtg cgccacggca attgtcgacc
     1981 gtacttttca aaccaggtca gcagttcgtg gcgcatggca cgcagggtat tgggatgcca
     2041 gtcgtgcggg gccaagggag tattcatggt gggggaagcg tccagacttc ggcggcgatg
     2101 agtccggagc cctgttcgat atcgatacga attcggtatt ctcctgtccg cggcacacag
     2161 aaggcgccgg attcgttggc gacagcgaag ggcgatgaac gggaatgtgc gtagccgacg
     2221 atggcctctt gggagtcgag aatcgatacg cgaaaggaat gcacctggtt atcggcggtg
     2281 acgaaaacgc gagcgcattg tgcggtggcc aggcggatgg aaaatgagcc gggggaagag
     2341 ggtgtggcgc ggtgaaagaa cgtgcggccg aagcgacgca tgccctgata aggagcgcac
     2401 gacagcgaca ggcgcgtcac atctcgttgg ggctggttgg aggtacgaaa accgttggtg
     2461 caagcagtcc atgcggggcc gtggaaaggg tggtccacgt ttgcgacggc cattggggcg
     2521 gcatcgaaag gcgtttgggt cgaggcgggt gaagcggaga cgaggggtag ggttgcatct
     2581 tgctgtcctg cttcgaacgc atcgggcgcg cttgttggta gttggcagct tgccgcggaa
     2641 aagaaagcca tgacaaagca aggggcaacg tgggcgcgag cggcgagttg cgcatcaagc
     2701 atggg
//
LOCUS       DAOSOR010000699         2704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_37666_length_2704_cov_4.886750, whole genome shotgun sequence.
ACCESSION   DAOSOR010000699 DAOSOR010000000
VERSION     DAOSOR010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37666_length_2704_cov_4.886750"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="PK710_23820"
     CDS             <1..1027
                     /locus_tag="PK710_23820"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT12829.1"
                     /translation="PKAYQAHYSLGVILERLGDADALARYRQAFTIVTDYEPAITAYG
                     LLLARKGNAAEADHFLTERHAKMPKSAAVAAALAEIKSMQRETESARKLAQEALKANP
                     NYAPAMITLARDHFRNRRLDLAKYALQAILDGFGPNNPPRDKDNVEARLLRALIYKEE
                     GARALAIGELESVLQKRPDMVDAGVQLATYYLESGNAAKAQPLLERALAYNRDNLVAH
                     LNLGDVHRLMGRPAEAKREFDWVLAKDASLIHVHYNIGLLFLFSDNYPGKTPVQQVDA
                     AIASFEKYKELRGRSVDPRGSDVEQLIIRAKAKKAMVEAESKQPSGPASPTSATPAAS
                     AAPAASQ"
     gene            1041..1280
                     /locus_tag="PK710_23825"
     CDS             1041..1280
                     /locus_tag="PK710_23825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12830.1"
                     /translation="MKHFCFATVIGLLLTSVSSFAHAQKQGRVITLNEVTIVGRVQRP
                     IAAVDVARIRPKITLSELRQPFLNKIEEAITKEPF"
     gene            1287..1841
                     /locus_tag="PK710_23830"
     CDS             1287..1841
                     /locus_tag="PK710_23830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12831.1"
                     /translation="MALWIASCCSVFAILWALWELRLAATALNLQPDLLCRACRSWRH
                     GLGILARAMQGEADAWETQLVRSLAQTREYEVARGEVNVVLSEIDISLSANTKTYAAC
                     ARISVFGCLIAAAWLFIAGQGLTTAVLDVFAIGAAGVLSTLAAAKEAQRLVRRKRQAL
                     DDWVEELIRLVQWEPQGESGVDVE"
     gene            1947..>2704
                     /locus_tag="PK710_23835"
     CDS             1947..>2704
                     /locus_tag="PK710_23835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOT12832.1"
                     /translation="MTQPYAEPPKSRPGQMTAVMRALPQATGPKVLRIGLVQGGRVIE
                     ERVVKQRTHVMVGASEKNMFVINAPGVPATFRLFELVGSDKKRAQYHLNYLDGMTGRI
                     ALQSGITDLQTLKSQAHRGPQGTYQVALTEEARGKIVIGDITFLFQFVAPPPVQPKPQ
                     LPVSVQSGLSSQIDWRFTIIAAFSFLFHFGAVGMLYSDWMDPEINEEAAIAGLLDSVR
                     NIPPPPPIETPDATTDATTTGTTEVATEQKGGGKG"
BASE COUNT          614 a          711 c          814 g          565 t
ORIGIN      
        1 tcccaaggca taccaagctc attattcgct tggagtcatc cttgaacgtc ttggggatgc
       61 cgacgctctc gcacgatacc gacaagcgtt caccattgtt acggactacg agccggcgat
      121 cacggcctac ggcttgttat tggcgagaaa gggaaatgca gcggaggctg accacttcct
      181 gaccgaacgt cacgccaaaa tgccgaaatc agccgcggtt gccgctgccc tggcggaaat
      241 caaatcgatg cagcgcgaga cggaaagcgc cagaaagctt gctcaggaag cgctgaaagc
      301 caaccccaat tacgctcccg cgatgattac gttggcccgg gatcacttcc ggaaccgaag
      361 gctcgacctc gccaaatacg ccttgcaagc catcctcgat ggtttcggtc ccaacaaccc
      421 gccgcgagac aaagacaatg tcgaagcgcg gctgctgcgc gccctcatct acaaggagga
      481 gggagcccgc gcgttggcga tcggcgaact cgagagtgtg cttcagaagc gaccggatat
      541 ggtcgacgct ggagtgcagc ttgcgaccta ctatctcgag tcgggcaatg cagccaaggc
      601 gcagccgctg ctcgagcgag cgcttgccta caatcgcgac aatctcgtgg ctcacctgaa
      661 ccttggcgat gtgcatcgat tgatgggaag gcctgcggaa gcgaaacgag aattcgattg
      721 ggttctcgcc aaagatgcaa gcttgattca tgtgcattac aatatcggac tgcttttcct
      781 tttttccgac aactatccag gaaaaacccc cgtacaacag gtcgacgcgg ccatcgcgtc
      841 ttttgagaaa tataaggaat tacgggggcg ttcggtcgat ccccggggca gcgacgtgga
      901 gcaattgatc attcgcgcca aagccaaaaa agcaatggtc gaagcggaat ccaaacagcc
      961 cagcgggccg gcatccccga cctcggcgac tcctgctgct tcagcggccc cagcagcaag
     1021 tcaatgagag gaaatgcaac atgaaacact tctgctttgc caccgtcatc ggactgttgt
     1081 tgacgagtgt atcatcgttc gcgcatgcgc aaaaacaagg ccgcgtgata accctcaacg
     1141 aggttaccat cgtggggcgc gtgcaacgtc ccatcgccgc ggtggacgtc gcgcgcatcc
     1201 gtccgaaaat cacgttgtcg gagcttcggc agcctttctt gaacaaaatt gaggaagcca
     1261 ttaccaaaga gcctttctga cacgtgatgg ctttgtggat agcctcgtgc tgcagcgtat
     1321 ttgcgatcct gtgggcgctg tgggagttgc ggctcgcagc gacggcattg aatcttcaac
     1381 ctgacttgct ttgtcgggcc tgtcggtcgt ggcggcatgg gttgggcatt ttggcgcgag
     1441 cgatgcaggg cgaggcggac gcttgggaaa cacagctcgt gcggtcattg gcgcaaactc
     1501 gtgaatatga ggtggcgcgc ggggaagtca atgtcgtgtt atcggagatc gacatatctc
     1561 tttctgccaa cacgaaaacc tatgccgctt gtgcgcggat ttctgttttt ggctgcctca
     1621 ttgcagcggc ctggcttttc atcgcgggcc aaggtctcac cacggcggtt ctcgacgtat
     1681 ttgccattgg ggccgcgggg gttctatcca cgctggccgc cgcaaaggaa gcccagcggt
     1741 tggtacgccg taagcgtcaa gcgttggatg attgggtgga ggaattgatt cggcttgtgc
     1801 aatgggaacc ccaaggtgaa agtggagtcg atgtcgaata ggcaagataa gacgcggact
     1861 atcgatcggg atgcaaccag aagcgttggg cttgcatctg gctcgagacg atgcgaaagt
     1921 tgtaatcatt gtttggagga ttggaaatga cgcaacctta cgcggaaccc ccgaaaagtc
     1981 gtcctgggca gatgacggcg gtgatgcgag ctttgccgca agcgacggga ccaaaggtat
     2041 tgcgtattgg tctcgtgcag ggcggtcgcg tgatcgaaga gcgtgtggtc aagcaaagga
     2101 cccatgtgat ggtgggggcg agcgaaaaaa acatgttcgt gatcaacgca ccgggggtac
     2161 cggccacgtt tcggttgttc gagttggtgg gttcggacaa gaagcgagcc caatatcatt
     2221 tgaattattt ggatgggatg accggccgaa ttgccttgca atcgggcatc acggatttgc
     2281 agactttgaa aagccaagcg catcgaggac cgcagggaac ctatcaagtt gcgctgacag
     2341 aggaggcgcg gggcaaaatt gtgatcgggg acatcacgtt tttgtttcaa ttcgtggccc
     2401 ctccccccgt gcagcccaaa ccccaactcc cggtgtccgt tcaatccggt ttgtcctctc
     2461 aaatcgattg gcgattcacg atcatcgcag cgttttcttt cttgtttcac ttcggcgcgg
     2521 taggaatgct ctactccgat tggatggatc cggagatcaa tgaagaagcc gcgattgcgg
     2581 gtttgctcga tagtgtaaga aacatccctc cgcccccacc gatcgagacg ccggatgcga
     2641 cgacggatgc tacgacgacg ggaacgacag aagtggcgac ggagcaaaaa ggggggggta
     2701 aggg
//
LOCUS       DAOSOR010000700         2696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_37818_length_2696_cov_4.245362, whole genome shotgun sequence.
ACCESSION   DAOSOR010000700 DAOSOR010000000
VERSION     DAOSOR010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37818_length_2696_cov_4.245362"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="PK710_23840"
     CDS             complement(<1..1037)
                     /locus_tag="PK710_23840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12833.1"
                     /translation="MSRIGESHRFFEHSSTRRGLNTMEMHRILALGSLFFIPLACHNP
                     STKQKSAPPSDSAIPISAPPKRIDPERPKSTVEPTVSDPTDGWPFVDDRDPANPEDAR
                     KRPFGIDALYRVQHLGDVHWSPDGKHVLFTVTTHDWKKGKSNTDIYLVDENGENLRQM
                     TRFEGTDAHPRWSPDGDSFLFVSSRGDESQVWVMPLQGGEPRAVSKISTGVSDPTWSP
                     DGTRIAFVSRVFPEHGADDAANAKQMKARKDNPIQAHIADHLLFRHWSFWADGTRNHI
                     LVLTIETGEVIDVTPGDFDSPSFELGGRGFAWSPDGREIVFVSNREAPDAQAWTTNKD
                     LWVVPSQGGEI"
     gene            complement(1034..>2696)
                     /locus_tag="PK710_23845"
     CDS             complement(1034..>2696)
                     /locus_tag="PK710_23845"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HOT12834.1"
                     /translation="EDKPKGNAAGKVLIISFLLGGLYDFLVEAVHAWNPMVKTSTLLG
                     GLGRRLHDQFRFEISLNAIAALFGLGYIIGLRYAAIIAAGSVLAYLVLVPLIYLVGAQ
                     LPEFQYIGETYKIAEMSGAQIFAQFVKPIGIGAIAVSGIIGMIRMGKIILGSLSLGLK
                     GLKSKSDDSEKVKRTDRDMNPRTVMLIQLGTTLLMGGLFFVVAWQKGDFGIGAAAGYA
                     ALGMIVAFVLSFLFTPVAAQAIAIVGVNPVSGMTLITLVIASGAMAASGLAGPAGMFI
                     ALIIGTAVCTALSTSGALVSDFKIGYWIGSTPANQQKWKFLGIIVASLTVAIVVPAMD
                     SAYHFLVADEAGRMVSNQEVLPAPQANMLAVIVKGLMSDAQQPYLLYALGGVVAIMLY
                     MAGVPMLAFALGMYLPIFINVPVLFGAFVAWLIGQTGGSKKVREARTSQGILIASGLM
                     AGAAIFGIVTAVLRLDFTGYAIRYLSIGEIFHVETKLIDGTKQTLLKSEAAAWYDTIL
                     GQGLGYGAYIILALACYFLARWGAEKELASSAPDTSDESRDQASS"
BASE COUNT          644 a          686 c          802 g          564 t
ORIGIN      
        1 atgatttcgc caccttggga gggcacgacc cacagatctt tgttggtggt ccaagcttgg
       61 gcatcgggtg cttcccgatt gctgacgaag acgatttccc tgccatccgg agaccaggca
      121 aatccgcgtc ctcccaattc gaacgatggt gaatcgaagt caccaggagt gacatcgatg
      181 acctcacccg tttcgatggt gagcacgaga atgtgattgc gggttccatc cgcccagaaa
      241 gaccaatgcc ggaagagcag gtggtcggcg atatgcgctt ggatagggtt gtctttgcga
      301 gccttcatct gcttggcatt ggcagcatca tccgcgccat gttcggggaa cacacgggaa
      361 acgaaggcga ttctcgtacc atcgggggac cacgtggggt cgcttacgcc agtggatatt
      421 ttgctcaccg cgcggggttc tcctccctgc aacggcatga cccacacttg tgattcatcg
      481 cctcgggaag aaacgaagag aaaagaatcg ccatcgggag accaccgagg atgggcatcg
      541 gttccttcga aacgcgtcat ctggcgaaga ttttccccat tttcatcgac caagtagata
      601 tccgtgttgc tcttgccttt cttccaatcg tgcgtggtga cggtaaacaa aacgtgtttt
      661 ccatccggag accaatgcac atcgccgaga tgctgcacgc gatagagcgc gtcgataccg
      721 aaggggcgtt ttcgagcatc ttcggggttg gcggggtcgc ggtcatcgac gaaaggccaa
      781 ccatccgtag ggtcggagac ggttggttcg acagtgcttt tgggtcgttc ggggtcgatg
      841 cgttttggag gcgcggaaat ggggatcgcc gaatcagaag gaggggcgga tttttgtttt
      901 gtggatgggt tatggcacgc caaagggatg aaaaatagcg atcccaaagc caggatgcga
      961 tgcatttcca tggtgttcaa tccccttcgg gtcgatgaat gttcgaaaaa tcggtgagat
     1021 tcaccgattc gactcatgaa gaagcttggt ctcgagactc gtcgctcgtg tcaggggcag
     1081 aggaagcgag ttctttttcc gctccccaac gagcaaggaa ataacaggcc agagcaagga
     1141 tgatataggc cccatagccc agaccctgtc cgaggatggt atcgtaccat gcggctgctt
     1201 cgcttttgag caaggtttgt tttgtgccat cgataagctt ggtttccaca tggaagattt
     1261 cgccaatgga aagatagcga atcgcatatc cggtaaagtc gaggcgaagc accgcagtga
     1321 cgattccgaa aatggcagca ccggccatga gcccggaggc aatgaggata ccttgcgagg
     1381 tgcgggcttc tcggaccttt ttgctgccac cagtctgtcc gatgagccag gccacgaaag
     1441 cgccgaatag cacgggaaca ttgatgaaaa tcggcaagta catccccagc gcgaaagcca
     1501 gcatcggcac gccagccata tacaacatga tcgccacgac acctccaaga gcgtacaaga
     1561 gatagggctg ttgggcatcg gacatcaggc ccttgacaat gacggccagc atattggctt
     1621 gcggcgcggg gaggacctcc tggttggaaa ccatgcggcc cgcttcatca gcgacgagga
     1681 agtggtaggc gctatccatc gcgggaacga cgatcgccac ggtgagggaa gcaacgataa
     1741 tgccgaggaa tttccatttt tgctggttgg cgggggtaga tccgatccag tagccgattt
     1801 tgaaatcgct cacgagggcg cccgaagtgg ataaggccgt gcaaaccgca gtaccgatga
     1861 tgagagcgat aaacataccc gcggggcctg ccaaaccaga ggcggccatg gcgccggaag
     1921 caatgacgag ggtgatgaga gtcattccgg atacggggtt gacgcctacg atcgcgatgg
     1981 cttgtgccgc gacgggagtg aaaagaaaac tgagaacaaa ggccacgatc atgccgagag
     2041 cagcgtagcc ggcggccgcg ccaatgccga aatctccttt ttgccaagcg acgacaaaga
     2101 acaatccgcc catcagcagg gttgtgccca actggatgag catgacggtt cgaggattca
     2161 tgtcacgatc ggttcgtttg actttttccg agtcatcgct tttgcttttg agccctttga
     2221 gtccgaggga caaggaaccg aggatgattt ttcccatgcg gatcatgccg atgatgcccg
     2281 agacggcgat ggccccgatc ccgatgggtt tgacgaattg agcgaagatt tgcgctccgg
     2341 acatctcagc gatcttgtag gtttcgccaa tgtattggaa ttccggcagt tgcgcgccta
     2401 cgagatagat gagaggaaca agaacgagat aggccagcac ggatccggct gcgatgatcg
     2461 cggcgtatcg aaggccgatg atgtagccga ggccgaaaag ggcagcgatg gcgttaaggc
     2521 ttatttcgaa gcgaaattga tcatgcaaac gcctgcccaa ccccccaagc aaggtgctcg
     2581 ttttaaccat cggattccat gcatgaaccg cttcgaccaa aaaatcgtac agaccaccga
     2641 ggagaaacga gatgatgagg acttttcccg cggcgtttcc cttaggttta tcctcg
//
LOCUS       DAOSOR010000701         2682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_38061_length_2682_cov_3.296155, whole genome shotgun sequence.
ACCESSION   DAOSOR010000701 DAOSOR010000000
VERSION     DAOSOR010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38061_length_2682_cov_3.296155"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..1082)
                     /locus_tag="PK710_23850"
     CDS             complement(180..1082)
                     /locus_tag="PK710_23850"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT12835.1"
                     /translation="MATLPWRLIATFVAAFDFARMALFGRWLGWFVGSFLRIRRVHVR
                     NAMRLAGLDASRSRVNRVYQQLGTGVFELLWAAGKAPSESAVLVRVDDWHQVERALSK
                     GRGVVVATAHTGNWDLAACAIAQRISLTVITKHLSWRSLDRYWQSLRAVRGVDLIDAA
                     GALRKTKERLAQGGAVVFLVDQAPHRSTGVVRFPFLGQMAEHDATFAMVAARYRVPVL
                     LALPKRSADGTHEVCVEWISDAPPRGSKTWVVQVTRAVSAKLETFVEQHPEQWLWLHR
                     RWKMHASNPPRGETDAQPSAGRRN"
     gene            complement(1087..1374)
                     /locus_tag="PK710_23855"
     CDS             complement(1087..1374)
                     /locus_tag="PK710_23855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12836.1"
                     /translation="MKQEQLEAFLASVSATVGEQGWHEVAAEQHITLYAGHDGVGLTV
                     GKVEAIQIEGDQLRARTRKGETYLLALSDVFATAVEAPKNNERKAGFAADR"
     gene            complement(1414..2241)
                     /locus_tag="PK710_23860"
     CDS             complement(1414..2241)
                     /locus_tag="PK710_23860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOT12837.1"
                     /translation="MAFRLCESEEEMGNLVDYETDRGIAIITLRDPPVNSFTHEMLSE
                     LDSYVLDARFDDDVRVIVVTGHGENYFSAGANITMLREVDPAFRKNFFLFAAETMSRI
                     ENTPKVVLAALNGHAVSGGFEIALACDFRIARAQAGSVGFPEIHLGLIPGAGGIQRLA
                     RMVGKGPAMQLVLEGEKLPFENAHQLGLIHQVWETATHAEFMANVIEYARRFASSDKA
                     PLALCKAKRAVQAAYENSIDQGLVVERSLLAPLLSSEDLQEGLAAWCEKRAAEFQGK"
     gene            complement(2382..>2682)
                     /locus_tag="PK710_23865"
     CDS             complement(2382..>2682)
                     /locus_tag="PK710_23865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT12838.1"
                     /translation="AISWLPVSWLHLTLMGRYVGAQYFQNPDTSRWGRLGSSRMLDAR
                     LEVLPLESLRLWVRGTNLTDANVQGHYSYPEPGRQVFTGLSLEWPEKKSDQGWTM"
BASE COUNT          635 a          840 c          657 g          550 t
ORIGIN      
        1 acgctgctcc atcatgacct cctacgtgaa tggatgaaaa gaattgttgt cgctcacaac
       61 ataagacgct cgctccccac gaaaccctcg accctctatc tgccatgaca ttggtcgatt
      121 gctccgctcg ccgccgaaac acaaaaggcc agaataagat aagtgtatgt aaataaacgt
      181 taattacgtc gtccggccga aggttgggcg tccgtttccc cgcggggcgg attcgatgca
      241 tgcatcttcc aacgacggtg cagccatagc cactgctccg gatgctgctc cacaaacgtt
      301 tcgagtttgg cagacacagc acgtgtgact tgcacgaccc acgttttcga tcctcgtggc
      361 ggcgcatcgc tgatccattc cacgcatact tcgtgcgttc catcggcaga tcgtttcggc
      421 aaagcaagaa gaacgggtac ccgataacgc gccgcgacca tggcaaatgt ggcgtcatgc
      481 tcggccatct gtccaagaaa gggaaaacga acaacgccgg tcgatcgatg gggagcctgg
      541 tcgacgagaa aaaccaccgc tcccccctgg gccaaacgtt ccttcgtttt tcgtaaagcg
      601 ccagcggcat cgatcaagtc gacaccacga acggcacgca acgattgcca gtagcgatcg
      661 agcgatcgcc aagaaaggtg ttttgtgatc acggtgaggg aaatccgctg cgcaatcgca
      721 caagctgcca ggtcccaatt gcccgtgtgg gccgtcgcca ccacgactcc cctacccttc
      781 gacaacgccc tttcgacttg atgccaatca tccacacgaa cgaggactgc actttccgaa
      841 ggggctttac ccgctgccca cagcaattcg aaaacgcccg ttccaagctg ttgatacaca
      901 cgattcacgc gagaacggga agcgtctagc cccgcaagcc gcatcgcgtt tctgacatgc
      961 acgcgacgga tacgcagaaa tgaacccaca aaccagccga gccatcgccc gaacaaagcc
     1021 attcgtgcga aatcgaaggc agcgacgaag gttgcgatga gacgccaggg aagcgttgcc
     1081 aagcctctat cgatccgcgg cgaaccccgc ttttctttcg ttgtttttcg gtgcttccac
     1141 cgcggtcgca aatacatcgg ataacgcaag caggtacgtt tcaccctttc gcgttcttgc
     1201 ccgaagctga tcgccttcga tctggattgc ctccactttg ccaacggtca aacccacacc
     1261 atcgtggcca gcgtacaagg tgatatgttg ttccgcggcg acttcatgcc acccttgttc
     1321 tcccaccgtc gcggatacag aagccagaaa cgcttcgagt tgctcttgtt tcatggcgag
     1381 atagagccaa cagaacggcc cggcgtcaag aaactacttg ccttggaact ccgcagcgcg
     1441 tttttcgcac caggcagcaa gaccttcttg caagtcttca gaggatagca gcggcgccag
     1501 cagggaccgt tccacgacaa gtccttgatc gatcgaattc tcatacgccg cctgcactgc
     1561 ccgtttcgcc ttgcatagcg ccaacggagc cttgtcggag gaagcgaaac gacgcgcata
     1621 ctcgatgaca tttgccatga actccgcgtg cgtggcggtt tcccagacct ggtgaatcaa
     1681 tccgagttga tgggcatttt cgaaaggtag cttctccccc tcgaggacca attgcatggc
     1741 cggccctttt cctaccatgc gagccagccg ttgaattcct cccgctcctg ggatgagtcc
     1801 cagatggatt tcgggaaatc caacgcttcc cgcctgcgct ctcgcaatac gaaagtcaca
     1861 agccagcgcg atttcgaatc ctccactgac cgcatgaccg ttcagcgcag ccaacaccac
     1921 cttgggggtg ttttcaattc gactcatggt ttccgcagca aaaaggaaaa aattctttcg
     1981 aaacgccgga tcgacttccc gcagcatcgt gatattggcc cccgcggaaa agtagttttc
     2041 tccgtgtccc gtaaccacaa tcaccctgac atcatcatca aagcgcgcgt ccaatacgta
     2101 gctatccaac tccgacagca tctcgtgcgt gaaactattc acgggaggat cgcgaagcgt
     2161 gatgatagct atgccgcgat ccgtttcgta atcgaccaaa ttgcccattt cctcctccga
     2221 ttcgcacaac cgaaaagcca tggtagcagc aacggcgacg aatcgttcag gattgttctc
     2281 gacggaatac cgcgccccga ttcaaacgtc ctatgcgctc atgaccgtcc ttcccaagct
     2341 ccccaagagc catgaacgcg aaaacacccg cctggtcctt ttcacatcgt ccatccctga
     2401 tcgctctttt tttcaggcca ttcgagggaa aggccggtga aaacctggcg ccccggttcc
     2461 ggatacgaat agtggccttg cacgttcgcg tccgtaagat tcgtgccccg tacccataac
     2521 cgaagcgact cgagggggag aacctccagc cgcgcgtcga gcatccgaga gctgcccaga
     2581 cgcccccacc gcgatgtgtc tgggttctgg aaatattgag ccccaacata acgacccatc
     2641 aacgtcaagt gtagccagga aacaggaagc caggaaatcg ca
//
LOCUS       DAOSOR010000702         2680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_38093_length_2680_cov_4.288381, whole genome shotgun sequence.
ACCESSION   DAOSOR010000702 DAOSOR010000000
VERSION     DAOSOR010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38093_length_2680_cov_4.288381"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(478..1377)
                     /locus_tag="PK710_23870"
     CDS             complement(478..1377)
                     /locus_tag="PK710_23870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12839.1"
                     /translation="MAWRFAVGFLLGWAFLPSMGCSHREPSDQGVASASSTVSAPAPV
                     QQPSRPSMGENVLVEKEDGQFVQGRVVKDGDPITVEYGGRKTKIRVSDDRVYGLGSRA
                     AAVTINDFAACKTAPDTWVGCLIKQVASGRVEVEDAQGKTYDLAEGDVVGLREETRRR
                     IGQKHKELSEHRDFFAAMNQQTRPYQPPGWEPKPGDEVVARHEDGSWRGGRIRRITPT
                     NVHVSWDDKSPLSIREAREVTPKPKKPQDVQPGQMVLARPRKGSVWELYRVQSIDAKG
                     VLIVDQDGRSRKARKSELIAFGS"
     gene            complement(1420..>2680)
                     /gene="polA"
                     /locus_tag="PK710_23875"
     CDS             complement(1420..>2680)
                     /gene="polA"
                     /locus_tag="PK710_23875"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOT12840.1"
                     /translation="SLQLHHLQRDRIERQGMSDLLTQVEIPLISVLIDMESAGVLIDV
                     IQLEQLGSMLQERMKELEATAKQQAGRDFNVGSPRQLETILFDELGLPVKKRTKSARS
                     TDASVLEALVDMHPLVGTILALRQLSKLQSTYVETLPKLVHPTTGRIHTRFNQSVAAT
                     GRLSSSDPNLQNIPVRTQEGLAIRRAFIAPQGYTLLSADYSQIELRVLAHLSKDPVLI
                     DAFRNHEDIHSRTAMEIFGVSSQQVTAEQRRQAKTINFGVIYGMGEVALSKKLGIERG
                     EARQFIDRYFSRYEGVRRFMDSTLEAARQGQAVETILGRRRYLPDLRSSNRVLRAQAE
                     RIAGNTPIQGSAADLLKLAMVRLAAPVVPGARMILTVHDELIFEVPENHVEEAENRVR
                     QAMESVMDLDVPLLVDVGVGPSWADAH"
BASE COUNT          563 a          840 c          661 g          616 t
ORIGIN      
        1 gttacaataa caccgttacc ttaacgtcgc aaccgactga tcggcatgta tttcggctct
       61 gagcacgatt ttgggaagcc ccctaacgcc gcgactgtgc aagcgccgac tgcacggggc
      121 tgccgcgtct gctacaaccc atattatgct gaatcgacga acgtaagccg tgcgcgcctt
      181 ggttcgatgc gttggtgatt cgccatcgcg tgagcaggga agctggaagg acggttttcc
      241 gctggaggtg agcgagtgtt gatctgattt atccgctacg tggcccatcc cccgcggaac
      301 cacctcccca caacccatgg cccttcgaat caccccgata gaccaacgtc aataccaccc
      361 aatacaccgc cctaagcgtg tatattgttg tatttattat agtttatcaa taccccgacg
      421 ttcgatggaa acgacacggt caccggagcg gattctcttg ctctctattg atcggaatca
      481 acttccgaac gcaatgagtt cgcttttacg agctttgcgg gaacgtccgt cctggtccac
      541 gatgagcact cctttggcat cgatactctg cacccggtac aactcccaca ccgacccttt
      601 tctcggacga gccagaacca tttgccccgg ttgcacatcc tgcggctttt tcggtttcgg
      661 cgtgacctca cgcgcttccc ggatcgaaag cggtgactta tcgtcccacg aaacatgcac
      721 gttcgtgggc gtgatgcggc gaatccttcc tccacgccag gagccgtctt cgtgtcttgc
      781 caccacttca tcgcctggtt ttggctccca tcccggcggt tgatacggtc gtgtttgctg
      841 gttcatggcc gcgaaaaaat ctctatgttc ggacaattcc ttgtgttttt gaccgatccg
      901 ccgccgcgtc tcctcccgta gccccaccac atcgccctcc gctaggtcat acgtcttgcc
      961 ctgcgcatct tccacctcta cccgtcccga agcgacctgc ttgatgagac agcccaccca
     1021 ggtatccggg gcagttttgc acgccgcaaa atcgttgatg gttaccgcgg ccgcacgaga
     1081 acccagccca tacacccggt cgtcggaaac cctgatcttc gtttttcggc cgccatattc
     1141 caccgtgatc gggtcgccgt ccttgaccac acggccttgc acaaactgcc catcttcttt
     1201 ttccaccaac acattctcgc ccattgacgg tctcgaaggt tgttgcaccg gagcaggcgc
     1261 cgacaccgtc gacgacgccg aggctacccc ttggtcggat ggttcacgat gggagcaccc
     1321 catcgaagga aggaatgccc aacctagcaa gaaaccaacc gcgaaacgcc acgccatggg
     1381 cgccgacgat agtgcaggtt gccccagagg caaggaccgt cagtgggcgt cggcccagct
     1441 tggtccaacc ccgacatcga cgagcaacgg cacatccaga tccatcaccg actccatggc
     1501 ctgacgaacg cggttttcgg cttcctccac atggttttcg ggtacttcga agatcaattc
     1561 atcgtgaacc gtcaaaatca ttctggcccc tggcacgaca ggagccgcca aacgaaccat
     1621 tgcaagtttc aatagatcgg cggcgctccc ttgaattggc gtatttcccg ctattcgctc
     1681 cgcttgggcg cgcaggacac ggtttgagct acgcaaatcc ggcaaatacc gacgtcgacc
     1741 tagaatggtt tccacggcct gcccttgacg tgccgcttcc aacgtcgaat ccatgaacct
     1801 tcgcacgcct tcgtaccgcg aaaaataccg atcgatgaat tgtcgagcct ccccccgctc
     1861 gatccctagt ttcttcgata gcgccacttc tcccattcca taaatcacac caaagttgat
     1921 cgtcttggct tgacgacgtt gctcggccgt tacctgttgc gacgatacgc caaagatttc
     1981 catcgcggtg cgcgaatgaa tatcttcatg atttctgaag gcatcgatga gcactggatc
     2041 tttcgataga tgcgccagga cccgcaattc gatctgcgaa tagtcggcgc tcaacaacgt
     2101 atagccttgt ggagcaatga aagcgcgacg aatggccagg ccttcttgcg ttcgcaccgg
     2161 gatattttgc aagttcggat cgctggagga aagcctaccc gtggcggcca cactttgatt
     2221 gaaccgtgta tgaatacgcc ccgtggtcgg atgaaccaac ttcggcaggg tttcgacata
     2281 ggtgctttgc agcttcgaaa gctgacgaag ggcaagaatc gtcccaacca acggatgcat
     2341 gtccaccaac gcttccagca cactggcatc ggtgctgcgc gcgcttttgg tccgcttttt
     2401 cacaggaagc ccaagctcat cgaagagaat cgtttcgagc tgtcgggggg aacccacgtt
     2461 gaagtcgcgt cccgcctgct gtttcgctgt agcttccaac tctttcatcc gctcttgcag
     2521 catcgatcct agctgctcga gctgaatgac atcgatcagc acgccagcgc tctccatatc
     2581 tatcagcacg gaaatcaacg ggatttcgac ttgcgtgaga agatcgctca tcccttgtcg
     2641 ttcgattcga tctcgctgca ggtggtgaag ctgcaaagac
//
LOCUS       DAOSOR010000703         2679 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_38114_length_2679_cov_4.342988, whole genome shotgun sequence.
ACCESSION   DAOSOR010000703 DAOSOR010000000
VERSION     DAOSOR010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38114_length_2679_cov_4.342988"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PK710_23880"
     CDS             <1..217
                     /locus_tag="PK710_23880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12841.1"
                     /translation="FATAFPEGHVEGWPESANSVVRLGLQISAACSTIAALVSFFGYG
                     RSDPLAHPLESPAPTPEPMVPGNIKTK"
     gene            complement(338..2038)
                     /locus_tag="PK710_23885"
     CDS             complement(338..2038)
                     /locus_tag="PK710_23885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOT12842.1"
                     /translation="MEAAISIVALILTVLGSLGVFLLGMKLISEALQKVAGERLKSLL
                     AKMTSNRFSGVLTGLAVTGVLQSSSATTVIVVSFVSAGLFTLTQAISVIMGANIGTTV
                     TGWIVALVGFKMEITLFALPAIAFGVFMSFMKHVKIKLWGEVLVGFGILFLGLSLMKD
                     SIPTISNPEQLAWLKHFSGGGLWSTLAFVLFGTVITAILQSSSATMTMTLTLAAMGWL
                     PYSAAAALVLGENIGTTATANLAAIGAPPVAKRAALAHLFFNVFGVIWAVALMNIYWL
                     PMVDWLVPGDPTVDFNTIQGDPAALALAAGVVSTHLAAAHTLFNVTNTAIMLPFVRQL
                     ERLVTRIVPETDDEAPARARFLDPSRIGSAEINVVQVSKAMQHMISLVRNMLKDAFYI
                     VAHPHDDLGKMVEKTLGIEREVDQLEQEVLSYLAALGQMPVSEATSRKLSDLVQNTHR
                     LERIGDHCAVIVRIARRIHSAGTPFSEEAIEDTHRLYEQVDRSLEHVSAFISGSGSIT
                     VGEEIEDRIDSTRRKLRGKYVQKMEQEGAQRAQCLAVLDTITHLEEIGDRAVGIIRRA
                     "
     gene            complement(2207..>2679)
                     /locus_tag="PK710_23890"
     CDS             complement(2207..>2679)
                     /locus_tag="PK710_23890"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOT12843.1"
                     /translation="IGSLAGAYINYYLAKWLGYPVLERYGKYFFLPKDKLERAQEIFR
                     QYGPGATFVCRLLPAIRQLISIPAGLSNMKLGPFTLWTGLGAGFWVTILTVVGYTIGA
                     HTASMSYADLVHKGKDQIGANLIYLIPTLLVGFALYVWISNRIMGKGKKVAVSG"
BASE COUNT          635 a          786 c          725 g          533 t
ORIGIN      
        1 cttcgccacc gccttccccg aaggccatgt cgaaggctgg cccgaaagcg ccaacagtgt
       61 cgttcggctc ggtttgcaaa tcagcgcggc ctgctccacc atcgccgccc tcgtttcctt
      121 tttcggatac ggccgctcgg accccctcgc gcaccccctc gaatcccccg ctcccacgcc
      181 tgaacccatg gttccgggaa acataaaaac caagtaatta caaatagtta taccaaatcc
      241 gacgttcgca ttctcgcctc aatagcccac ttggcctgcg agcatgcatc cacgagcctc
      301 cacggtgccc cgagcgtccc gtcgacgcca caaagactca cgcccgccgg atgattccca
      361 ccgctctatc cccaatctcc tcgagatggg tgatggtatc caacaccgcg aggcattgcg
      421 cccgctgagc cccctcctgt tccattttct gcacgtattt tccgcggagt tttcgacggg
      481 tagaatcgat cctatcctcg atctcctcgc ccacggtgat acttcccgaa ccggatatga
      541 aagcgcttac gtgttcgagg gagcggtcca cttgttcata aagacggtgg gtgtcttcga
      601 tggcttcctc cgaaaacggt gttcccgcgg aatgaatgcg tcgcgcgatg cgcacgatga
      661 cggcacaatg gtctccaatg cgttcgagtc ggtgggtgtt ttgcaccagg tccgagagct
      721 ttcgtgaggt agcttccgaa acaggcattt ggccgagagc cgccagatac gacaggactt
      781 cctgttcgag ttggtcgacc tctctttcga tccccagcgt tttttcgacc atttttccga
      841 ggtcatcgtg gggatgggcg acgatataaa aggcgtcttt gagcatattt cgaaccaacg
      901 agatcatatg ctgcatggcc ttgctcacct gaaccacgtt gatttcggcg cttccgatgc
      961 gcgaagggtc gaggaatcgg gcacgggcgg gtgcttcgtc atcggtttca ggaacgattc
     1021 gggtgacgag gcgctcgagt tgacgaacga aaggcagcat gatcgcggta ttggtgacat
     1081 tgaatagcgt gtgggcagcg gccagatgcg tggagacgac gccagcggcc aaagccaatg
     1141 ccgccggatc gccctgaatg gtattgaagt ccacggtagg gtctccgggc acgagccaat
     1201 ccaccatggg aagccaatag atgttcatga gagcgaccgc ccatatgaca ccaaagacgt
     1261 tgaaaaacag gtgagcaagg gctgcccgct tggccaccgg aggcgctcca atagcggcaa
     1321 gattggcggt tgccgtcgtg ccgatgtttt cgccaagaac caacgctgcc gccgcggaat
     1381 agggaagcca tcccatggcc gcaagcgtca aggtcatggt catggtcgcc gagctggact
     1441 gaagaatcgc cgtaatgacc gtcccaaaca gcacgaatgc gagggtcgac cacagtcctc
     1501 cccccgaaaa gtgcttgagc caggcaagtt gttcggggtt cgagatggtg gggatcgaat
     1561 ccttcatcaa cgataggccg agaaataaga taccgaaacc gacgagcacc tcgccccaga
     1621 gcttgatttt tacgtgtttc atgaagctca tgaacacgcc gaaggcaatc gcgggcaggg
     1681 caaaaagcgt gatttccatc ttgaagccga ccaacgccac gatccacccg gtcaccgtgg
     1741 tgccgatatt ggcgcccatg atgacggaaa tggcttgggt gagcgtaaac aagccggccg
     1801 aaacgaagct gacgacaatg acggtggtgg ccgaactgga ttgcagtacc cccgtgaccg
     1861 caagacccgt gagcacaccg ctgaaccggt tcgaagtcat tttggccaac aatgacttga
     1921 gacgctcgcc cgccaccttc tgcaaagctt cggaaatgag cttcatcccc agaaggaaaa
     1981 cgccgaggga accaagcacc gtgagaatca gggcgacgat cgaaatggct gcctccacat
     2041 ccaagccctc atggcaattc gcaaagaaaa gcagcgaggg gtggaagggg cctcatcggc
     2101 ccagaaaagc gctcatcgtg taccacagcc gctcggaagg ccaatgggaa aacagcttgc
     2161 atcggtcgat ttttcagcgt ggtatcgccg aggaaaagag cttcattcag cccgaaacag
     2221 ctactttctt gcccttgccc atgattcgat tgctgatcca cacataaagc gcaaacccca
     2281 cgagcaaggt cggtatcaaa tagatgaggt tggcgccgat ctggtctttt cccttatgaa
     2341 cgaggtccgc atagctcatc gaagccgtat gggcgccgat ggtatagcca acgacggtca
     2401 atatcgttac ccagaatccc gcccccaaac ccgtccacag cgtaaatggg cccaatttca
     2461 tgttcgaaag tcctgccgga atcgaaatga gctgacgaat tgccggaagc aaccggcata
     2521 cgaacgtcgc cccagggccg tattgccgga agatttcctg cgctcgctca agtttgtcct
     2581 tcggcaaaaa gaaatactta ccgtaacgct cgagcacagg atatccgagc catttcgcca
     2641 agtaatagtt gatatacgca ccggcaagcg aacctatcg
//
LOCUS       DAOSOR010000704         2677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_38150_length_2677_cov_3.807018, whole genome shotgun sequence.
ACCESSION   DAOSOR010000704 DAOSOR010000000
VERSION     DAOSOR010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38150_length_2677_cov_3.807018"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..472)
                     /locus_tag="PK710_23895"
     CDS             complement(50..472)
                     /locus_tag="PK710_23895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12844.1"
                     /translation="MNSRRTKPSVLHLMGEMNGRSKTCGSAMIAVFLLWQVIAVLHLA
                     FAIHTVCPEHHVVAHADRETGEVEHHPDVPFSDDDECFVLVALAAHGGMPCLDAPQVP
                     VAPSYLLETEPQIIRSIILAQRYRFRLSPSHSPPAAGG"
     gene            complement(677..1756)
                     /locus_tag="PK710_23900"
     CDS             complement(677..1756)
                     /locus_tag="PK710_23900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12845.1"
                     /translation="MPTTDGFDLDETRPFANVPWEAPATEPQPVVVMHAHDDVMVEMG
                     ETELDLDDVGLGGLEIVVEDDVFKAEPPSSSRRVLEPEFDLSDDSRVTPPPESGPQRV
                     VPKIEVDDSPRTPTPVEHPSIEMLGQTVDLPESGKDKLELDHTPKASPVREVTTTEME
                     AVIPGASPPGIFPPAEPSTKDWDLPDDFASAPEKPTEPLRKKPMPIAMDAPFAEGIIS
                     PIDDVAVKEREVAEKAAQEAKKREEAERAAQEAKERQEAEKAVQEAKKRQEAEKAAQE
                     AKEREEAERAAQQAKERQEAERAAQEAQRRALEARPVVVEVAGKTVECADLSAAEVAT
                     FVGAIQAESTKTFVQALDASLALGQ"
     gene            complement(1900..2469)
                     /locus_tag="PK710_23905"
     CDS             complement(1900..2469)
                     /locus_tag="PK710_23905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12846.1"
                     /translation="MADAPFDPSGAITFDLPSGCVNLAHASARVLVPADGVATLCHAA
                     GEHATREFGYSIGTAMGKRLAQRLGQGAANVSMQTFLQHLRGEFALVGFGVVGMQQWA
                     DALLLIVEHTSLPSDLIAATLESALAGSTNRTVVCVKLVEEDGKTRFLLGGPQGAKRV
                     TEWLEKGMFWGEVLVRLHSRHDPDARGDA"
BASE COUNT          537 a          806 c          739 g          595 t
ORIGIN      
        1 taccaattta tttccataaa taagcggaac gtgcttcgtg gctgtagtac tagccaccag
       61 cagcgggagg agagtgagaa ggagaaagac ggaaacgata acgttgagcg aggatgatgc
      121 tcctaatgat ttgcggttcc gtctcgagca aataactcgg agccaccggc acctggggag
      181 catcgagaca cggcatgccg ccatgcgctg ccaaagcgac gagcacgaaa cattcatcat
      241 catccgagaa cggaacatcc ggatggtgct cgacttcgcc cgtctcccgg tccgcatgag
      301 ccaccacatg gtgctcggga cacaccgtgt gtatggcaaa cgccagatgc agtacggcga
      361 tcacctgcca aagcaggaag acagctatca tcgcgctgcc gcaggttttc gatcgcccgt
      421 tcatctcacc catcaggtgt aagaccgacg gtttggttcg ccgcgaattc attccgacga
      481 acacattctg tcggaagtgt tagcgcggat ccttgaaccg gtcaatgcga tgcttcctgc
      541 cgtcccgtgg gccaaatccc acctgggaag ccacaggcgc ttctgctatt tatagtgcaa
      601 aatcaatatg ttacagaaaa cgcctcagcg gattttcgac cgctatcagg tatgtgacat
      661 ccgccagagc gacaggtcat tgccctagcg ccaggcttgc atcaagagcc tgaacgaacg
      721 ttttcgtcga ttcagcctga atcgctccca cgaaggtagc cacctcagcc gcggacaggt
      781 cggcacattc cacggttttt ccagcaacct ccaccaccac cgggcgagct tcaagagcac
      841 gtctttgcgc ctcttgcgcc gccctttcgg cttcttgacg ctctttggcc tgttgcgccg
      901 ccctttcggc ttcttcacgc tctttggcct cttgcgccgc cttttcggct tcttgacgct
      961 ttttggcttc ttgcaccgcc ttttcggcct cttgacgctc tttggcctct tgcgctgccc
     1021 tttcagcttc ttcacgcttt ttggcctctt gcgccgcctt ttcggccact tcgcgctctt
     1081 tgacggcaac atcgtcgatg ggggatatga ttccctcagc gaacggcgca tccatcgcaa
     1141 taggcatggg cttctttcga agtggctcgg tgggcttttc aggagccgaa gcaaaatcgt
     1201 caggcaaatc ccaatctttg gtggatggtt cggcaggagg gaagattccg ggaggcgatg
     1261 ctccaggaat caccgcttcc atctcggtgg tcgtcacttc gcggaccgga ctggctttcg
     1321 gcgtatggtc tagctcgagc ttgtctttgc cgctttcagg aaggtcgacc gtctgtccca
     1381 gcatctcgat cgatgggtgt tcgacgggcg tcggcgttcg gggactgtcg tcgacctcga
     1441 tcttcggaac cacccgttgt gggccagatt ccggaggtgg cgtcacccgc gaatcatcgg
     1501 acaaatcaaa ctcgggctcc agcacacgcc tcgaggacga aggcggttcc gctttgaaca
     1561 catcgtcttc gactacgatc tcgagccctc ccaagccgac atcgtcgaga tccagttcag
     1621 tctcccccat ctcgaccata acgtcatcat gagcatgcat gacaacgacc ggttgcggtt
     1681 ctgttgccgg tgcctcccac gggacgttgg caaagggtcg tgtctcgtcg agatcgaaac
     1741 cgtccgtggt tggcatggag atggatgacc gcgtagctcg agccgcagaa tccaagacga
     1801 agggactttg tggagggacc atcggggcac gctcgcgact ggccctctgt tgaaccctgg
     1861 tcaggagaga ctgcagtttc gctatttttt cctgacggtt catgcgtcac ctcgcgcatc
     1921 gggatcatgt cgggagtgca atcgtacgag cacttctccc caaaacatgc ccttttcaag
     1981 ccattcggtc acacgtttcg ctccttgggg ccccccgagc aaaaagcgcg ttttgccgtc
     2041 ttcttccacc agttttacgc atacgaccgt gcggttggta gagcccgcca gagcgctttc
     2101 caatgtggcg gcaatgagat cagagggtag gcttgtgtgc tccacgatga gaagcaacgc
     2161 gtctgcccac tgctgcatac ccacaacgcc aaagcccacc aaggcgaatt cgcctcgtag
     2221 gtgttgaagg aacgtctgca tcgagacgtt ggcagcgccc tggccgaggc gttgcgcgag
     2281 acgttttccc atcgcagttc cgatggaata tccgaactcg cgtgtggcat gctctcccgc
     2341 ggcgtggcaa agggttgcca ccccatctgc tgggacgaga acccgcgcgg aagcgtgggc
     2401 aaggttcacg caaccggaag gaaggtcgaa ggtgatggct ccggaaggat caaacggggc
     2461 gtcggccatg ggcactccca ccgcacaagg tttatcaagc gttgtaaagc gttgtagcca
     2521 accgtgtcaa atgcagagcc atcgaaccgt cttccaagct gcactttgcc tatccttgta
     2581 cggcctggca ggtccggctt tggcccaaaa tcagtgggtg gaaccggcac aacccgcccc
     2641 gatgctcaac gaacgacctg gcggttctta ccacgcc
//
LOCUS       DAOSOR010000705         2663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_38400_length_2663_cov_4.396472, whole genome shotgun sequence.
ACCESSION   DAOSOR010000705 DAOSOR010000000
VERSION     DAOSOR010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38400_length_2663_cov_4.396472"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="PK710_23910"
     CDS             complement(<1..1091)
                     /locus_tag="PK710_23910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin"
                     /protein_id="HOT12847.1"
                     /translation="MIVVRVGCLGAALMMSTMPAAAECDNAARLSTCIDADTLWLHPG
                     PGRFQAIGSTETTASEEFSLGFFTTYFRNPIVLRASSANPDGVDVPAIEHMVNSTFLW
                     GYGLSDRVELTLAMPVTLYQNGTGVSGFLSSQSQEVPTTSMRDLRLGATWSLLPRSRV
                     HSDELFALTVRMQAAIPTGDEQWFGGDRGFVGIPSLAGDMRLGRFFAATELGVRIRKT
                     TNLAGSRIGSQSFLALGAGVDLLPSEWLSVGVEAYALPGLVGQETLERDPANGELIGK
                     TKSESLIPAEWMTTLRSAPTRGGDLSFYISGGSALPLDGTAMTAPQMRWTAGVRFAPL
                     HRDSDGDGVIDSLDQCPYQPEDRDGFQDE"
     gene            1229..1450
                     /locus_tag="PK710_23915"
     CDS             1229..1450
                     /locus_tag="PK710_23915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12848.1"
                     /translation="MSCPYRAVLLVTRDMRGTASSVAKLRIELHCGEPEGHLGAHHDP
                     RHGIQWDDHGKTVRTLLRDESEPARSHFP"
     gene            complement(1508..>2663)
                     /locus_tag="PK710_23920"
     CDS             complement(1508..>2663)
                     /locus_tag="PK710_23920"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT12849.1"
                     /translation="KWKHQFQSAGFYAAVEQGYYRTAGIVVEIWEATGQGDPADVVLR
                     GDAQFGIASSDLIILRAQGKPVVALAPIYQHSPMVLLVSEKSGIDSVHGLKGKRIMLE
                     SHADELIAYLHFEGIERGAVTWIPHVFDPSRLIDGTVDATSAYSTDEPFLLQIEGMRY
                     STYTPRSGGIDFYGDTLFTTEEQVRMHPDRVRRFLEASLEGWKYAMAHEQEVIDLILN
                     KYSKRHSREHLLFEAERSRQLIMGDVVEIGYVNPGRWQHIGETYQRMGTIQSVPSLSG
                     FLYERNPQHVPRGLYGALVGAFALTLIIAAVAYRFYRLSVQVREQAESLRKAMAEIKV
                     LSGIIPICAHCKKIRDDQGYWNQLEAYISNNSDALFSHGICEDCEKKYYP"
BASE COUNT          624 a          809 c          678 g          552 t
ORIGIN      
        1 tcctcgtcct gaaatccatc ccgatcctcg ggttgatagg ggcattggtc caggctgtcg
       61 atgaccccgt cgccatcgga gtcacgatgc aaaggcgcaa aacgaacccc cgcggtccat
      121 cgcatctgag gcgcggtcat cgccgttcca tcgaggggca acgcggatcc gccgctgatg
      181 taaaatgaca aatcgcctcc acgtgtcgga gccgatcgga gcgtggtcat ccattccgcg
      241 ggaatcaaag actcggactt ggttttgccg atgagttcac cattggcagg atcgcgttcg
      301 agcgtttcct gcccgaccaa tcccggcaag gcatacgcct ccactccaac gctcaaccac
      361 tcggaaggca ataggtcgac ccctgccccc aacgcaagga aactctggga acctatccga
      421 ctgcccgcga ggtttgtcgt cttgcgaatt cgaaccccca gctccgtcgc cgcaaaaaaa
      481 cggcccagcc tcatatcccc agccaagctt ggaatcccga caaagccccg atcaccgcca
      541 aaccattgct catcacccgt gggaatcgcg gcctgcatac gcaccgtgag cgcgaaaagt
      601 tcatcggagt gtacccgtga acgcggaagc agagaccacg tggctcccag acgaagatcc
      661 cgcatcgaag tcgtgggtac ttcctgggac tgactcgata aaaaaccgct cacccccgta
      721 ccgttttggt aaagcgtcac aggcatcgcc agcgtgagtt ccacccgatc ggaaagacca
      781 tatccccata gaaacgtcga gttcaccatg tgctcgatgg caggcacgtc gacgccatcc
      841 ggattggccg atgacgcgcg aagaacaatg ggatttcgaa aataggtcgt gaaaaagcct
      901 agggaaaact cctccgacgc cgtggtttcc gtcgaaccga tggcttgaaa ccgtccgggg
      961 cccggatgaa gccaaagcgt atcggcatcg atgcatgtgc tcaaacgagc cgcgttgtcg
     1021 cattccgcag ccgctggcat ggtgctcatc atcaacgcag cgccaaggca accgactcgc
     1081 accacgatca tcgcccgatg ccgtggccgg aaacccaatg aagaagggga tcgtgaaacc
     1141 atgaattccg ttgctctatc acgatggccc caccggggaa acgcctccct tgacgacgtt
     1201 cccgcttcac gtctaggggt ggagcatcat gtcttgtccg tatcgcgccg tgcttctggt
     1261 gactcgcgac atgcggggaa ccgcgtctag tgttgccaag cttcggattg aattgcactg
     1321 cggagaaccg gaaggtcacc tgggcgccca tcacgatcca cgtcacggaa ttcaatggga
     1381 cgaccatggg aaaaccgtac gcacgttgct tcgcgacgaa agtgaaccgg cgcgaagtca
     1441 tttcccatga tactcgtggc ggtttcgctt gatcggatcc catttcgcga aggtggaacc
     1501 aaatcgttca gggatagtat ttcttctcgc aatcctcaca tatgccatgg ctaaagaggg
     1561 catcggagtt gttggagata taggcttcga gttgattcca ataaccctgg tcgtcacgga
     1621 tcttcttgca atgcgcacaa atggggatga taccgctgag cactttgatc tccgccatcg
     1681 ctttgcgcaa tgactccgct tgttcgcgca cttgcacact caatcggtag aatcggtacg
     1741 ccacggcagc gatgatcaac gtcagcgcga aagctccgac aagcgcccca taaagccccc
     1801 gcggcacatg ctgcggattt ctctcataca aaaaacccga aagcgatggc accgattgga
     1861 tggtgcccat ccgttgatac gtttccccaa tatgctgcca tcgcccaggg ttgacatatc
     1921 caatctccac gacgtccccc atgatgagct ggcgagaacg ctccgcttca aataacaaat
     1981 gctcccgtga atgccgtttg ctgtacttgt tcaggatcaa atcgatgacc tcttgctcat
     2041 gggccatcgc gtatttccat ccttccagcg atgcttccaa aaaccttcgc acgcgatcgg
     2101 gatgcattcg aacttgctct tccgtggtga ataacgtgtc tccgtaaaag tcgatacctc
     2161 cggatctcgg ggtatacgtg ctgtatcgca tcccctctat ttgcaacaaa aatggttcat
     2221 cggtcgaata ggccgacgtg gcatccaccg tgccatcgat caatctggaa ggatcgaata
     2281 catggggaat ccacgtgacc gcaccacgct cgatcccttc gaaatgcagg taagcgatga
     2341 gttcgtcggc atgactctcc aacatgattc gcttgccttt gagcccgtga acactatcga
     2401 ttccagactt ttcggacacc aaaagcacca tcggagagtg ttggtagatg ggcgccaacg
     2461 ccaccacagg cttgccttgg gctcgcagaa tgatgagatc ggaactggcg ataccaaact
     2521 gcgcgtcgcc acgaagcacg acatccgcgg gatccccttg acccgtggct tcccatatct
     2581 cgaccacgat gcccgcagtt cgataataac cttgctcaac cgccgcataa aatcccgctg
     2641 attgaaattg atgcttccat ttg
//
LOCUS       DAOSOR010000706         2653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_38589_length_2653_cov_3.949962, whole genome shotgun sequence.
ACCESSION   DAOSOR010000706 DAOSOR010000000
VERSION     DAOSOR010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38589_length_2653_cov_3.949962"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="PK710_23925"
     CDS             complement(<1..1248)
                     /locus_tag="PK710_23925"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOT12850.1"
                     /translation="MDPIKLFIRRPIFTAMLLLALVVFGLFSWPKMGVDMMPDMDIPV
                     VTVVTVLPGADPETIEKDVTEPLEEVFNTLPGLHTLTSVNVENVSQIIIRFDMERDID
                     VAAQDIRDRVQSTLSKLPTDIQTPLIQKLDPGAMPILTLAFSGPLSAERMSNFAEDEL
                     KPALQQINGVGTVTIFGDQQSEVRITLDPTRLRAHGLTPLDAVGAVRAQDLDLSSGRT
                     AESHVERIVKLKAEARSVEELRTLVLSSPMGIPVRLGEVADVTQGPAEPRSLARLGSS
                     AAIGLTVTKQSGANTVQVVSNILAGIAGLQQRLPEGCELQVVHDDSKYIRSSITAVQE
                     DMGIGGVLAVVVVLIFLRNWRSTLVSAVALPTSVIGTFAFMHLLGFTFNIVTMLALTL
                     SIGLLIDDAIVVIENVVRHLENGV"
     gene            complement(1264..2361)
                     /locus_tag="PK710_23930"
     CDS             complement(1264..2361)
                     /locus_tag="PK710_23930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT12851.1"
                     /translation="MKLREILFLGAMAALVPFAVGCENHGAKAALPSVASAQPVPVRV
                     VKPETQSLSGESRVTSSLRSKSEAILSAKYTGTIIFLEVQVGDRVKAGQALVRLDASM
                     AAIALQNAKAADRLAQANLANAKTELERAKALREQGALSDANFDKLNMAHDIASAQAD
                     QAKAAIRMASQQISDATIVAPFAGVVSARFRNPGDTVSLMPPTPVLSLVDPDHLEVKM
                     AVPEALVPRLRVGDVLGATASPLDTAFQVRVSALGATVDPMTRTVEVLADVMEPMDPN
                     LKPGALATVDLAGSKVLAGLYLPSAAVHKADGQSFVYVVKEGKLQRKQVVIAPIRPGT
                     ILVTDGLRSDDQVALDMVELAEGQTVRPLSN"
BASE COUNT          609 a          862 c          651 g          531 t
ORIGIN      
        1 caccccgttt tccagatgcc gcaccacgtt ctcgatgacc acgatcgcat cgtcgatcaa
       61 tagaccgatc gacagcgtca acgcgagcat cgtaacgata ttgaacgtaa atcccagcaa
      121 gtgcatgaat gcgaaggtgc caatgaccga ggtgggaagc gccaccgcgg acaccagggt
      181 cgagcgccaa ttgcgcaaaa aaatgagcac caccaccacc gccaacacac ccccaatgcc
      241 catgtcctcc tgcaccgcgg tgatggaact tcgaatatac ttcgaatcgt cgtgaacgac
      301 ctgcaattca cagccttcgg gcaaacgctg ctgaagcccc gcaatacccg ccaggatatt
      361 ggatacgacc tgaaccgtat tggcccccga ttgcttggtc acggtcaagc caattgcggc
      421 agaagaccca agacgcgcca gactgcgcgg ctccgccggc ccctgggtca cgtccgccac
      481 ctcgccaagc cgcacgggga ttcccattgg gctggacaaa acgagcgtcc gcaattcctc
      541 gacagagcgt gcctccgcct tgagtttcac aatcctttcg acatgagatt cggcggttct
      601 tccactggat aaatcgagat cttgcgcccg aaccgcaccc accgcatcca acggcgtgag
      661 cccatgcgcc cgcaacctcg tcggatccaa cgtaatgcgc acctcgctct gctgatcacc
      721 gaaaatcgtc accgtcccca cgccgttgat ctgctgcagc gctggcttca gttcatcttc
      781 cgcaaaattc gacatccgct ccgccgacaa cggaccactg aacgccaagg tgagaatggg
      841 catcgccccg ggatccaact tctgaatcaa aggcgtctga atgtccgtcg gcagcttgga
      901 cagcgtcgat tgcacccgat cccgaatatc ctgagcagct acatcgatat cgcgctccat
      961 atcgaatcgg atgatgatct gggatacgtt ctcgacgttg accgaggtga gcgtatgaag
     1021 ccccggaagc gtgttgaata cctcttccaa cggttccgtc acgtccttct cgatggtctc
     1081 cggatcggcc cccggcagca cggtaaccac cgtgaccacc ggaatatcca tgtccggcat
     1141 catgtccacg cccatcttcg gccaggaaaa taaaccgaaa acgacgaggg cgagtagcag
     1201 catcgcggtg aaaatcggtc ggcgaatgaa tagcttgata ggatccaaca cagcctccga
     1261 tcgtcagttc gacaaggggc gaacggtctg cccctccgcc agttccacca tgtcgagagc
     1321 cacctgatcg tcagaacgca aaccatcggt gacaaggatc gtcccaggac ggatcggtgc
     1381 tataactacc tgttttcgtt gtaattttcc ttccttcacc acatacacga aagactgacc
     1441 gtcggcttta tgaaccgctg ccgacggcaa atacaaccct gccaaaacct tcgaacccgc
     1501 cagatccacc gtcgcaagcg ctcctggctt caagttcgga tccatcggct ccatcacgtc
     1561 tgccaatact tccaccgtgc gtgtcatcgg atcgaccgtc gcccccaacg cgctgacacg
     1621 aacctgaaac gccgtatcca acgggctggc ggtggccccc agcacatccc ccacacgaag
     1681 ccgcggcacc aaggcctcgg gcactgccat tttcacttcg agatggtcgg gatccacaag
     1741 cgaaagcacg ggggtgggcg gcatgagact caccgtatca ccgggatttc tgaaacgcgc
     1801 ggagacaacc cccgcaaacg gcgccacgat cgtcgcatcg ctgatctgct gactcgccat
     1861 ccgaatcgcc gccttggcct gatccgcttg tgccgacgca atatcatgcg ccatgttcag
     1921 cttgtcgaaa ttggcatcgg aaagcgcccc ctgctcacga agcgctttcg cccgctcgag
     1981 ttccgtcttt gcattggcca gattcgcctg ggcaagacgg tccgctgctt tcgcattctg
     2041 caacgcgatc gccgccatcg acgcgtccag gcgcacgagt gcttgaccgg ctttgacccg
     2101 atcgcccacc tgcacctcga ggaatatgat cgtacccgta tacttcgcgc tcaagatcgc
     2161 ttcgcttttc gatcgaaggc tggacgtcac ccggctctct cccgacaacg attgggtctc
     2221 gggtttgacg acacgaacgg gcacgggttg cgcggacgca acagagggaa gcgccgcttt
     2281 ggcaccgtga ttctcacagc cgaccgcgaa cggaaccaac gccgccatcg cgccaagaaa
     2341 caaaatttca cgtaatttca tgatgataac caatacacgc gtatcgtgga ataagcttgc
     2401 cgtttccttt ggagccatgg gcgcttgctt cgatgcacga ccatcgcgta cgaacggtca
     2461 tctcggcaac ccggccaggg catcgatggg ggaacggttc atcgtatgaa ccctagatca
     2521 ggataatgaa acctcaaatc attaattgaa cccatgttca tattggtttt tcttccaccg
     2581 tcaccgtatt tttcggcttc ccatcccgcg atcatactgt aacctactga aataattact
     2641 atatttctat ttt
//
LOCUS       DAOSOR010000707         2621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_39176_length_2621_cov_3.904521, whole genome shotgun sequence.
ACCESSION   DAOSOR010000707 DAOSOR010000000
VERSION     DAOSOR010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39176_length_2621_cov_3.904521"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PK710_23935"
     CDS             <1..695
                     /locus_tag="PK710_23935"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT12852.1"
                     /translation="VGAGAFFRGSSDGIANEGPARSITLSAFWIDKYEITTARYARCV
                     DAGACKPTATGRWCNQGNAAKRDHPINCVRWDQAEAYCRWAQARLPTEAEWEKAARGS
                     DGRVYPWGNEPPDCERTVWFDPDTRLHACGKFGTWLVGSKPKGASPYGAMDMAGNVWE
                     WVADAYQADYYAIAPSKDPPGPNAGSYRILRGGGWGHDGRGALRAAKRFPFAGSNQTP
                     GIGFRCAMDDET"
     gene            complement(810..2366)
                     /locus_tag="PK710_23940"
     CDS             complement(810..2366)
                     /locus_tag="PK710_23940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HOT12853.1"
                     /translation="MLRSSHNLDLIPHLPRYLSAVGAFACLMLAPGPSEAQVVLQDNL
                     TSASGCTGPCVSRTVEGAGSFSAAGWQTLHAHDRIIYDIGQELSCGVLQFTLANFAPW
                     PQYQGTTTYKNQGINFLGLFENSEGSHGTAIGANESVFMLKYAANLDWNGSEYVLADP
                     GLEWVRQTVGWVAGLQVGPCDQGASCANNPEFDWAEAQMANSAPDTPSTYVYRLEWNT
                     TGIRVLINGTERAAHPFVVYPNCCHLQPDVPRLRYLYVGKTTFLGTDGWGYFKGPIYS
                     NITVERCQDPCEGHCGNGIQDCGEEGIDCGGTCPPCNTPPKTGKIRVYDASGARLDEG
                     GPPAHRGDTITLRAAVDDDETEDKNMVNPRFFLRSSGSAEWNVLDGAAATFDPNEPEL
                     NWFYTWTIPMTAPLGVYDIRFDYADEAGAVATQTQTDEFTVIDALTPDGGTQEGGAGE
                     AGTTHDAGSDARPDSSSSAEASPEGSPSAASSDDSDGCGCRIGSRPSGSWLWLGLLGL
                     GWLRRRRAHR"
BASE COUNT          564 a          652 c          860 g          545 t
ORIGIN      
        1 gtgtgggagc aggggctttt tttcggggtt catcagacgg cattgccaac gaagggcccg
       61 cgcgttcgat cactctttca gccttttgga tcgataaata cgagataacg acggctcgct
      121 acgcccgatg tgtcgacgcc ggagcgtgca aaccgaccgc gaccggcagg tggtgcaacc
      181 agggaaacgc cgcgaaaagg gaccatccga tcaattgtgt tcgatgggat caagccgagg
      241 cctattgcag gtgggctcag gcgcgtctgc cgaccgaagc agagtgggaa aaggcggcca
      301 gggggagcga tggcagggtg tatccgtggg gaaatgagcc tccagactgc gaacgtaccg
      361 tatggttcga cccggatacc cgcctgcacg cttgcggtaa gttcggaacc tggttggtgg
      421 gctccaaacc caaaggagca tcaccctacg gtgcgatgga tatggcgggc aacgtctggg
      481 aatgggttgc ggatgcctat caagctgatt attacgccat cgcgccgtcg aaggatcccc
      541 ctggcccaaa cgcggggagc tatcgtattc tgcgcggtgg aggctggggg catgatggaa
      601 gaggggcgct cagggcagcc aagcggtttc cgtttgcagg gagcaaccag acgccgggca
      661 taggatttcg gtgtgcgatg gacgacgaaa cgtgaggcga gcagcggctc ccgggaagtg
      721 taacatcttg aatttatgaa tttatttgtg ttagcggcag aatgggtttg gccgtgccgt
      781 tggctaggaa ctcaatcctt cgtggatggt taccgatggg ctcgacggcg tcgcagccag
      841 cccaggccca gcagtccgag ccatagccag gagccgctgg ggcgagagcc gatccggcat
      901 ccacaaccgt cggaatcatc gctcgaagcg gccgaagggg aaccttcggg agaggcttcg
      961 gcggaagagg aggaatcagg acgagcgtcg ctaccggcgt cgtgggtcgt cccggcttct
     1021 ccagctcctc cttcttgggt gccaccatcc ggcgtaaggg catcgatgac ggtaaattcg
     1081 tcggtttggg tttgggttgc gacggcacca gcttcatccg cgtaatcgaa gcggatatca
     1141 taaaccccca ggggcgccgt catggggata gtccaggtgt agaaccaatt gagttcgggc
     1201 tcgttggggt cgaaggtggc ggcggcgcca tcgaggacgt tccattcggc gcttccgctg
     1261 ctccgaagga agaaccgagg gttgaccatg tttttgtcct cggtttcatc gtcatcgacc
     1321 gcggctcgca aggtgatggt atctccccga tgagcgggcg gaccgccttc gtccaaccgg
     1381 gcgccggaag cgtcgtatac acgtattttg ccggttttgg gtggtgtatt gcaggggggg
     1441 caggtccctc cgcaatcgat gccctcctct ccacaatctt gaataccgtt tccgcagtgt
     1501 ccctcgcagg ggtcttggca gcgctcgacg gtaatgttgg aatagatggg gcctttgaaa
     1561 tagccccagc catccgttcc tagaaacgtg gttttcccga catataaata acggagtctc
     1621 ggaacgtcgg gctgtaaatg acagcaattg gggtaaacga cgaaaggatg ggcagccctt
     1681 tcggtgccgt tgatgaggac acggattccg gtggtattcc actcgaggcg gtagacatag
     1741 gtacttgggg tgtcgggggc ggaatttgcc atttgggctt ctgcccagtc gaactctgga
     1801 ttgttggcgc aagaggcgcc ttggtcgcaa gggccgactt gtagaccggc aacccagccg
     1861 accgtttggc gaacccattc gagccctggg tctgccagga cgtattcgct tccgttccag
     1921 tcgaggtttg ccgcgtactt gagcatgaat accgattcgt tggcgccaat cgcggtgcca
     1981 tggctccctt ccgaattctc gaaaagacca aggaaattga tgccttggtt cttgtaggtg
     2041 gtggtgcctt ggtactgagg ccacggagcg aagttggcga gggtgaattg aagcacgccg
     2101 caagaaagct cttgtccaat atcgtagatg atgcggtcgt gggcatgcag ggtttgccag
     2161 ccggccgcgg agaaactgcc agctccttcg accgttcggc tcacgcaagg gcccgtgcag
     2221 ccagaggcgg atgtcaggtt gtcctgtagg acgacttgtg cttcggaagg gcctggtgcg
     2281 agcatgaggc aagcgaaggc cccgacagca gaaagatagc gtggaagatg ggggatgagg
     2341 tcaaggttat gggatgaacg aagcatgcat gattcctttc tcaaggcgag tctaagctcg
     2401 aaaatgctga agcaagcgag gtaatacagt gatttcgaaa aaaattactc aaactgtacc
     2461 aatgactgga tcggtttgtc aaggttgaaa aaagaatggt tagtgaaaag ataatgtaaa
     2521 atccaagtcg gatcgaagag gaaggacaag ccattgggcg acgaatgggg catgatcatc
     2581 gatctggaac cgcagtttgc ctgcgactcg gagaggccct g
//
LOCUS       DAOSOR010000708         2614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_39320_length_2614_cov_2.934740, whole genome shotgun sequence.
ACCESSION   DAOSOR010000708 DAOSOR010000000
VERSION     DAOSOR010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39320_length_2614_cov_2.934740"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="PK710_23945"
     CDS             complement(<1..1030)
                     /locus_tag="PK710_23945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12854.1"
                     /translation="MKPTHWVLLSLSTLIACHSPPPPPENVTLNPVQEKTSPPSKPPP
                     PPPPLVPPPKAVRLTTGLEGFCAVLDNGKVSCWGGSPEKPIPRILPNIDNAVDVTRLT
                     INPHDELIVIARTDGSVLSLAEGQEIPNNFKYLNNVQRVHGNRSIADGTLFGGVLCAL
                     HRDGTVSCFPRGAMQPEAKPLSMPGWTDVLELSVSLNHVCARMPSGIQCVRHGDLGTS
                     KVVRVPLPEGKTAVSMQVGFSVLCVAFESKTVACQDLGAPKASFKEVPGANPFGLNQL
                     GWLEVDVPIVCATNGNHVTCSRMDEFGSGVSLPALEKGDVSLEGFGSVEELAIAPQAA
                     CLRNSDGAI"
     gene            complement(1290..1751)
                     /locus_tag="PK710_23950"
     CDS             complement(1290..1751)
                     /locus_tag="PK710_23950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12855.1"
                     /translation="MIVVACRTPHHSEGLPDSSTTAASATTMRHPMREIENTHAQPGE
                     TEKPRLVLCLTASPQMQVGYGHVLECQIHKVLAGAFEENTLRLTILAGDHDRLKYLLA
                     HAYPTVFTIGFTLHRSDEPYPLMPLSGFVDSHRYSWAIDFMNDACHNPTTP"
     gene            2048..>2614
                     /locus_tag="PK710_23955"
     CDS             2048..>2614
                     /locus_tag="PK710_23955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12856.1"
                     /translation="MDNIVYFLTDASDEQAREAAAAMEQTARELAPILAREVRFHVEN
                     VLPRALAKLRLHGADVLVVDARHEAPTEQSKAVQLLRELYREHEVAGPTSRSRTILVA
                     PASPFGAQIAFEAGRLRIGSTLPLEAQDSGWARIWHHIDRAIHERRGGKTAICLAGGG
                     IEGGFYEVGILRALQYFLPNFSLANVDII"
BASE COUNT          538 a          642 c          826 g          608 t
ORIGIN      
        1 caatggctcc gtccgagttc cgaaggcatg cggcttgtgg ggcaatcgcc aattcctcta
       61 cgctgccaaa cccttccagg gagacatcac ccttttccaa ggcaggcaag gagacacccg
      121 agccgaattc gtccatgcgg gagcaagtca cgtggttgcc attcgtggcg cagacgatcg
      181 gaacatccac ttcgagccat ccgagctggt tcaacccgaa cggatttgcc cccggcacct
      241 ctttgaagga ggctttggga gcgccgagat cttgacaagc caccgttttc gattcgaaag
      301 ccacgcataa gaccgagaat ccaacctgca tggaaaccgc tgttttccct tccggcaggg
      361 gtacgcggac caccttcgac gtgcccagat ctccgtgacg gacacattga atccctgagg
      421 gcatgcgcgc acaaacatga ttgagcgaga cggaaagctc gagaacgtct gtccatccgg
      481 gcatggaaag gggtttggct tctggctgca tcgctccccg ggggaaacag ctcacggtcc
      541 catcgcgatg aagagcgcat aagacgccgc cgaacagggt accgtcggct atgctccggt
      601 tgccatgaac tcgttggaca ttgtttaaat atttgaaatt attgggtatt tcttgacctt
      661 cggcgagtga taggacagaa ccatccgtac gagcgatcac gatgagttcg tcgtgtggat
      721 tgatggtgag tcgagtcaca tccacggcgt tatcgatatt cggaagaata cgggggatgg
      781 gcttttccgg acttcctccc caacagctta ctttgccatt gtcaaggaca gcgcaaaagc
      841 cttccaagcc tgtcgtaagg cgaacggctt tgggcggtgg gacgagaggt gggggcggtg
      901 ggggtggttt ggagggtggg ctggtttttt cctggaccgg gttgagcgtt acgttttcgg
      961 ggggaggggg tggggaatgg caggcgatga gcgtggacag ggaaagcaaa acccaatggg
     1021 ttggtttcat gggatcaatg ctatcacctt cggggggagc atggttgttt gcattgattg
     1081 aatccgcatg tttttctcgt ttggacggcc gtgggagcgg cttgggggtg tcgttgggca
     1141 agccgatggg ctcggggtca tggtaggcgc agggcgcggt tggttcggcg tatgcggttt
     1201 ggagtttgcc tttcgatgac ttgtgcgggg gctgctatca ggggccaaag tcctgtcgac
     1261 gaagcccgtc catgcggagg ctttcgcatt cagggggtcg ttgggttgtg gcaggcatcg
     1321 ttcatgaaat cgatggccca ggaatatcgg tgagaatcca cgaaaccgct gaggggcatg
     1381 agggggtaag gttcatcact gcgatgcaat gtgaaaccga tcgtaaatac cgtaggatag
     1441 gcatgcgcaa gcaaatactt cagcctatcg tggtcacccg ccaggattgt gagacgtagc
     1501 gtgttttctt cgaaggctcc tgccaacacc ttgtgtattt ggcattctaa tacgtgtcca
     1561 taccctacct gcatctgagg ggatgccgtc aagcataaaa ccaatcgagg tttttccgtt
     1621 tccccgggtt gtgcatgggt attttcaatt tcgcgcatcg gatgacgcat cgtcgtggcg
     1681 gatgccgcgg tcgtggaaga atccgggagt ccttccgagt gatggggagt gcggcaggca
     1741 acgacgatca tcgcgcaccc ccaaagcacc ggactatttt ccagcagctt cgaaaggatt
     1801 gaggatgggc cccggcgcat agaataggga gtagcggttt tctctcgcgc catcaatacg
     1861 caagcggccg caaccgtatc aacgcgggtc ggatggtgtg cgaaaaacct tgtatcgaat
     1921 cgatggtcgt ttcgtcgtga attggcggac ggttatcgtg gctgcgtcat gaacttcatc
     1981 gcagctcgcg cgcccgtttt gttctgccgt gtgttatact gttgtatctt tgcatcgttg
     2041 actgcccatg gacaacattg tctattttct aacggatgct tcagacgagc aggcgcggga
     2101 agcggcggct gccatggagc aaacggcacg agagttggcc cccatcttgg caagggaggt
     2161 gcgttttcac gtggaaaacg tattgcccag agcgcttgcg aagcttcggc tccacggagc
     2221 tgacgttctt gtcgtcgatg cgcgtcatga agcgccgacg gagcagagca aggcggtgca
     2281 actattgcgt gagttgtatc gcgaacatga agtggcgggg cctacgagtc gttcgagaac
     2341 gattctggtc gcgccggcaa gcccattcgg tgcgcaaatc gctttcgaag cagggcgact
     2401 gcggattggt tccacgttgc ctctcgaggc gcaggacagc ggatgggcaa ggatatggca
     2461 tcatatcgat cgggccattc acgaacgacg tggcgggaaa acggcgattt gtcttgcggg
     2521 tgggggcatc gaaggcgggt tttacgaagt cggtatcctt cgtgctttgc agtattttct
     2581 gcccaacttt tccctggcta atgtcgatat catt
//
LOCUS       DAOSOR010000709         2600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_39600_length_2600_cov_3.956778, whole genome shotgun sequence.
ACCESSION   DAOSOR010000709 DAOSOR010000000
VERSION     DAOSOR010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39600_length_2600_cov_3.956778"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PK710_23960"
     CDS             complement(<1..236)
                     /locus_tag="PK710_23960"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOT12857.1"
                     /translation="MVAVSGGAGFIGSHLVRSLVAEGARKVIILDSLRYGDPSNLAGL
                     GAKVELVPFTLGTDDPGRLPDLLAGVSGLFHLAA"
     gene            637..2079
                     /locus_tag="PK710_23965"
     CDS             637..2079
                     /locus_tag="PK710_23965"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOT12858.1"
                     /translation="MPPPHSRKTPWLRVCLWLGLLAFLFTGVSLVAIAFVVLPAEGCP
                     AHGLFVGSHLPPSDPPFGSWLEQRRQRLSQEPVLLVYGMDSYETTLGELGIEVDVAAT
                     MADALRFGREPSVAQRARTLYLARNSLIDVPLSYSMDMRRAQITLRPFAQQVRRAPVN
                     AYVNLTEHARIADIPGAELDLEATAASMMQGVLAGERAFLVHTRPIRAQVTEADLMAV
                     DVTRVVSTFETRFTTWGEGHGRSRNIELAAGSIDGMVLMPGQSFSFNEVVGRRSLEAG
                     YVWAPVIVGDEMRPGVGGGICQVASTLYASALLGALQITERWAHGRPSSYTRMGLDAT
                     VAYPSKDLRFVNSLPYPVILHLHFPEKGVLRAEILGGEPIAKVRYSYGVSRTMPFVRR
                     IVSVSSLAPGTAKRKQKGIQGYSVVSVVELDFGSRVDKRVYHSEYRPTPEIFWVSPDY
                     DLSALPELPKGAKGIEEKITEAEQEYDPTG"
     gene            complement(2140..>2600)
                     /locus_tag="PK710_23970"
     CDS             complement(2140..>2600)
                     /locus_tag="PK710_23970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOT12859.1"
                     /translation="APNATIIGDVEIEEGASIWFGVVLRGDVGPIRIGAGSNIQDLTC
                     AHTTGGVSRVIVGRQVTVGHSCVLHGCEVGDEALIGMGSVLLDNAVVGEGSVLGAGSL
                     LTARTAIPARSLAMGRPARVVRPVNDSERTLGIDGAAVYQELMKEYRGHT"
BASE COUNT          535 a          828 c          682 g          555 t
ORIGIN      
        1 tccgcggcaa gatgaaataa gccactcacc cccgccagca agtccggcaa tcgaccggga
       61 tcgtcagttc ccagcgtaaa aggcaccaac tcgaccttcg cgccgagacc ggcaagattg
      121 gatggatcgc cgtagcgaag gctgtcgagg atgatgacct tccgagcccc ctcggcaacg
      181 aggcttcgga cgagatgcga gccgatgaag ccggcgcccc cggatacggc aaccatggat
      241 cctgcaaagc gagatggcat gcctctggat agcataacga ctggcgcttg tgaccccctg
      301 ccgaaccgtg gacgatagga tcggttctcg aaagggatac catgaatcgc cgcgaggccc
      361 cccgcggttc catctcataa taattataaa atcaattatt tatgttattt tcattcccct
      421 cggcctcgaa tcgtcgcaag cccatgccat cctcaaaacc aacgatgctc aagcgggcca
      481 gcgcatgccc ggatcacaaa aatcatcttt atttacaaat ggttatctat tcaccgacag
      541 tctttggacg ctcctatcgt tcaccttgcg cgaattacgc cctctcgcgc cctcctcctt
      601 tttgtcaaac cgcgcgccaa cagctaccct ttgagggtgc ctccccctca ctcacgaaag
      661 accccatggc ttcgtgtctg cctctggctg ggactcctgg cctttttgtt cactggcgtt
      721 tctctcgtcg cgatagcgtt tgtcgtcttg cccgcagaag gttgcccagc tcatgggctt
      781 ttcgtgggtt ctcatcttcc cccttctgac ccaccgttcg gttcctggct cgaacagcgg
      841 cgtcagcggc tctcgcaaga accggtgttg ctcgtatacg gcatggattc ctatgaaacc
      901 acgctcgggg agctgggaat cgaagtcgac gtggccgcaa ccatggccga tgctcttcga
      961 ttcggtcggg aaccgtccgt cgcccaacgc gcacgcacct tgtatttagc tcgaaacagc
     1021 ttgatcgacg tgccgctatc ctattccatg gatatgcgtc gcgcgcaaat caccttgcga
     1081 cctttcgcgc agcaggtgcg acgggcaccg gtcaatgctt acgtcaacct tacggagcat
     1141 gctcgaatcg ctgatattcc gggtgcggag ttggatttgg aagccaccgc ggcctcgatg
     1201 atgcaaggcg tgcttgccgg tgagcgagct ttcctcgttc atacccggcc cattcgcgct
     1261 caggtcaccg aagccgatct gatggccgtc gacgtcaccc gcgtggtatc gaccttcgaa
     1321 acgcgattta ccacctgggg agaaggccac gggcgttccc gcaacatcga acttgccgcc
     1381 ggctcgatcg acggcatggt gctgatgccg gggcaatcct tctctttcaa tgaagtcgtg
     1441 ggacgccgtt ctttggaggc cggatatgtg tgggcccccg tcatcgtggg cgacgagatg
     1501 cgtcctgggg tgggaggagg catttgccag gtagcgagca ccttgtacgc ttccgccctg
     1561 ttgggtgccc tacaaatcac cgaacgatgg gcccatggcc gtccatcgag ctacacccgt
     1621 atgggcctcg acgccacggt agcttatcca tccaaggacc tgcgtttcgt caattcgctg
     1681 ccctatcctg tgattctcca cctgcacttt ccggaaaaag gggtattgcg agcggaaatc
     1741 cttgggggcg aaccgattgc caaggtgcgc tactcctacg gcgtttcccg aacgatgcct
     1801 ttcgtgcgac gtatcgtctc cgtatcctct ctcgcccctg gaacggcaaa acgaaaacag
     1861 aaaggaattc aagggtattc cgtcgtttcg gtggtggaat tggactttgg ttcgcgcgtg
     1921 gataaaaggg tttatcactc ggaataccgt ccaacaccgg aaattttttg ggtgtccccg
     1981 gactacgacc tgtccgcgct gccggagctt cccaaaggtg ccaaaggaat agaggaaaaa
     2041 atcacggaag ccgagcagga atacgatcca accggatagt cgacgacctg ctccgtggtt
     2101 tgcgccatct tggtgcggac tcgcgacatc cccaaaactt catgtgtgtc cgcgatattc
     2161 tttcatcaac tcctgataca ccgccgcccc atcaatcccg agggtgcgtt cgctatcgtt
     2221 gaccgggcgc accacccgag cgggacggcc catcgccaga gatcgagcgg ggatcgcggt
     2281 tcgagcggtc aacaggctcc ctgcccctag cacggaccct tcccccacca ccgcattatc
     2341 cagcaggaca ctgcccatcc cgatcaaggc ctcgtcgccc acctcgcaac cgtgcaagac
     2401 gcaactatgg ccaaccgtaa cttggcgacc gacaatgacg cgggacacgc ctccggtcgt
     2461 atgcgcgcat gtcaaatcct ggatattcga ccccgcgcca atccgaatcg gccctacatc
     2521 accgcgaagt acgaccccaa accatatcga agcgccttcc tcaatctcca catcaccgat
     2581 gatcgtcgca ttcggcgcca
//
LOCUS       DAOSOR010000710         2586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_39879_length_2586_cov_2.883050, whole genome shotgun sequence.
ACCESSION   DAOSOR010000710 DAOSOR010000000
VERSION     DAOSOR010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39879_length_2586_cov_2.883050"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /locus_tag="PK710_23975"
     CDS             <1..1422
                     /locus_tag="PK710_23975"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT12860.1"
                     /translation="TITGGASYPQQMRALRMEPSIIIGTPGRLRDHIERGFIDMSHVR
                     SVVLDEADQMLDMGFREDLEAIVDRAAPGRRMHMASATFPPDAQALAKRYQVDSRTVF
                     GAEPGEINTDISFSAHRVRPSDRFAALVNVLLLQPEERTLVFVRTRADAATLAQQLAE
                     AGFLARPISGDLEQAERTRTLEAFRSGIIKVLVATDVAARGLDIADVSRVIHADPPGD
                     AEALTHRSGRTGRAGKKGHSIILVPPAAEEAVTRMFSRAKVHVRWTAVPTEEQVWKAA
                     DERLLASLQDALHAHDDPDSEPEPDRLHALAERLLREARDPVPLVRELLKRAHHGGPS
                     EPRKVETVVVERHKRMLSKEHADRDSWLPFRINYGARAGADARRLLAMVCRRGRIQGS
                     QVGAIRIGEAFSTFEVQAQVVEAFLQHVRKPDPRDPHIRISRFDPQYFKGKGTPRPAR
                     EPKAHLLVKRFAKHHQAKHRGQG"
     gene            complement(1447..>2586)
                     /locus_tag="PK710_23980"
     CDS             complement(1447..>2586)
                     /locus_tag="PK710_23980"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HOT12861.1"
                     /translation="RLEDAIRQVETGKNLDGARKSLEALLQDPSLTASAKDDARLALS
                     RALELQGDREGAIVQVETLLGSHPGSKHFESRSTAERRLSKLLNNDDREPRQPTREEQ
                     VAPMAGVLSKFFPPAANGSYSVRILVFGRPPESSETLGTFNIAGAIRRAAEDACPLCE
                     NKVRMDNTHSGFSSWTSLPMVLAASSSDNPSLADSLTVFYFDLEQLRVPGRYDRYLPL
                     AADQIASYLHPEQGVVVAKLRENAPPIIVIAAPRWGLLSEVERAFSEMTELPTEPKPI
                     ALTHGLRSSEIRNTIRAAYPSLRQCYEKHLEKSPEAAGKFIVGFEIDDRGAVGMARME
                     PNSTLTDAELQQCFLGVVRGLSFPAVGGKTTVTYPIELRPNRATN"
BASE COUNT          546 a          724 c          797 g          519 t
ORIGIN      
        1 accattacgg ggggagccag ctatccccag caaatgcgcg cgcttcggat ggagccctcc
       61 atcatcatcg ggactcccgg aaggcttcgg gatcatatcg aacgggggtt catcgatatg
      121 tctcatgttc gaagtgtggt tctcgacgaa gccgatcaga tgctggatat ggggtttcgc
      181 gaggatctgg aggcgatcgt ggatcgagcg gcgccgggga ggcgaatgca catggcttcc
      241 gcgacatttc cgccggatgc ccaggcgctt gccaagcggt atcaagtcga ttcacggacg
      301 gtcttcggtg cggaacctgg cgaaatcaat acggatattt cgttttcggc gcatcgggtg
      361 cggccttccg atcgattcgc ggccctggtc aatgtgctgc ttctgcagcc cgaagagcga
      421 accctggtgt tcgtacgcac gcgcgccgac gccgcaaccc ttgcgcaaca actcgcggaa
      481 gcaggctttt tggcgcgccc catcagcgga gacctggaac aagccgagcg aacgcgaacg
      541 ctcgaagcct tccggtccgg tatcatcaag gtgttggtgg ccacggatgt ggcggcaaga
      601 ggtttggata tcgccgatgt gagtcgggtg attcatgccg atccgccggg agatgcggaa
      661 gcgctgaccc atcgttccgg acgaacgggc cgagcgggta agaaaggcca tagcatcatc
      721 ctcgttcctc ccgcggccga agaagcggtt acgcgcatgt tttcccgtgc gaaagtgcat
      781 gtccgatgga ccgccgttcc caccgaagag caggtttgga aagccgcgga cgagcggctc
      841 ttggcatcct tgcaagatgc cctgcacgcg cacgacgatc cggattcgga gcccgaaccc
      901 gatagacttc acgcgctggc ggagcgtctt ttgcgcgaag cccgggaccc ggtcccgttg
      961 gttcgggaac tgctaaaaag agcgcatcac ggggggccat cggaaccacg caaggtggag
     1021 acggtcgtgg tggaacgcca caagcgaatg ctatcgaaag agcatgcgga tcgcgattcc
     1081 tggcttccgt ttcgcatcaa ttacggggct cgcgcagggg cggacgcgcg acgattgctg
     1141 gccatggtct gcaggcgagg ccggattcag ggctcgcagg tgggggctat ccgaatcggg
     1201 gaagcgtttt ccaccttcga agtgcaggcc caggtcgtgg aggcgttcct tcagcacgtt
     1261 cgaaaacccg atccacgaga tcctcatatc cgtatttcgc gcttcgatcc ccaatatttc
     1321 aagggaaaag gaacgcccag accggctcgt gagcccaagg ctcatctcct tgtcaaacgc
     1381 tttgccaagc accaccaggc aaaacatcga ggccaaggct gaggaagtca aactatccgt
     1441 atatggttag ttcgtagcgc gattgggtcg caattcgatg ggatacgtga cggtcgtttt
     1501 ccctcccacg gcagggaaag agagcccgcg aacgacgccg agaaagcatt gttgcagctc
     1561 ggcatccgtc aaggtggagt tgggttccat gcgagccata ccgaccgcgc cgcgatcgtc
     1621 gatttcaaat cccacgatga acttgccagc ggcttcaggc gatttttcca agtgtttttc
     1681 gtaacattgg cgcaacgagg gatacgccgc gcgaatcgtg ttgcgtatct ccgaagagcg
     1741 caagccatgg gtgagcgcaa tgggtttggg ttccgtgggg agttctgtca tttcggaaaa
     1801 tgctcgttcc acctcggata atagccccca gcgcggagcc gcgatcacga tgatgggagg
     1861 cgcgttttca cgtaacttgg cgacaactac cccttgctcc ggatgcagat aggaggcgat
     1921 ttggtccgcg gccagcggaa gataccgatc gtagcgtcca gggactcgaa gctgttccag
     1981 atcgaaataa aagaccgtca gcgagtcggc caaagaagga ttgtcagaag acgaggcggc
     2041 aaggaccatg ggcaaggacg tccatgagga aaaccctgaa tgggtattgt ccatccgcac
     2101 tttgttttcg catagcgggc aggcgtcttc cgcagcgcga cgaatcgctc ctgcgatgtt
     2161 gaatgttcct aacgtttcac tgctttctgg aggacggcca aaaaccaaga tgcgaacgga
     2221 gtaagagcca ttcgcggcag gagggaaaaa cttggatagc acaccggcca tcggggccac
     2281 ttgctcttca cgcgtgggtt gacgcggctc acgatcgtca ttgttcaaca gcttgctgag
     2341 gcgacgttcc gccgtggaac ggctttcgaa atgcttggaa ccaggatggg agcccagcag
     2401 agtctcgact tgcacgatgg ccccttctcg gtccccttgc aattccaagg cgcggctcag
     2461 cgccagacgc gcatcgtctt tcgccgaagc cgtaagcgat ggatcctgca acagggcttc
     2521 cagtgatttt cgcgcgccat ccaggttttt tcccgtctcc acttgacgaa tggcgtcttc
     2581 gagacg
//
LOCUS       DAOSOR010000711         2581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_39984_length_2581_cov_3.939826, whole genome shotgun sequence.
ACCESSION   DAOSOR010000711 DAOSOR010000000
VERSION     DAOSOR010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39984_length_2581_cov_3.939826"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="PK710_23985"
     CDS             <1..1112
                     /locus_tag="PK710_23985"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOT12862.1"
                     /translation="ESLGVQMSVLIGNVALWEELVAVRQRLERENRYYREGNPAPVAS
                     GRMVGDSPAFRHLIELIGKVAPTRSAALILGETGVGKELVAQEIHRLSAHRNGPFIAV
                     HVASLPPGLVASALFGHERGAFTGATEQVRGRFELANGGTIFLDEIGELGVEDQVRLL
                     RVLQEGTFERVGGTKTLRSSFRLVAATNRDLAALVREAKFREDLYYRLNVFPVVVPPL
                     RDRLEDIPKLAFHFLDQYARRAGKEYEGIGEADMDRLLEYAWPGNVRELEHVIERAVV
                     LSDPPMLKIPTLATSREPSSSRTPKEFRRLEEVERQYIREVLNHVGGKITGKAGAAEI
                     LGMKPSTLQFRIDKLGLREALDQSRYQGKAARRKS"
     gene            complement(1155..>2581)
                     /locus_tag="PK710_23990"
     CDS             complement(1155..>2581)
                     /locus_tag="PK710_23990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT12863.1"
                     /translation="SNTTVPWRIGAAREWDRSILRERRTPAFKLPQPIPTGVFSRAPR
                     QILGRRILAQALLALAFAWYREHNHFSKEEPLTTPQHLSLYTSRHDRVEVCVTVKASV
                     PNASLSQLASSRAGQSYLAELQSIAPLTREEEWQLALRIENSEQAMLQAILEVPDLAR
                     QVCSLLLAAADGQQPIHQLLEYSQNDEVAHARLRRLVSALTSRNNDNISELLQNIRFH
                     RKRLEDLANQVHPETHPEAAARIAKARRTIQTTKRELVRRHLWLAVRIAIRLPSRRLD
                     LLDRIQEGNLGLMQAADRFQVRKGVRFATYAAFWVRRGIHRATAEQGYCVRLPVQVLE
                     AAYRAEQSQRRHDAKHPDPASEQHIAQSAAMSVERLREVKRGIGDSVSIDDHDIADPM
                     PSVEQQIGDWQRVQVLHAQVASLDNREKRVLTLRYGLESGEELSREDTANVFRLTTER
                     IRQLENQALQRLQHPCRKTLLLAV"
BASE COUNT          549 a          635 c          815 g          582 t
ORIGIN      
        1 tcgagtcctt gggcgtccaa atgagtgtat tgattggaaa cgtggcattg tgggaggagt
       61 tggtggcggt tcgacagcgt ttggagcggg agaaccgata ttatcgagag ggaaatcccg
      121 cgccggtggc ttctggccgc atggtcgggg attcgcctgc gtttcgccac ttgattgaat
      181 tgattggaaa agtggctccc acacgaagcg cggctctcat cctaggagaa acgggcgtgg
      241 gaaaagaact cgtagcccag gagatccatc ggttgagcgc ccaccgaaac ggccctttca
      301 ttgccgtgca tgtggcatcg ctccctccgg ggttggtggc aagtgccctt ttcggccatg
      361 aacggggggc ttttacggga gccacggagc aagtgcgagg acgattcgaa ttggccaatg
      421 gggggacaat ctttctcgat gaaatcggtg agcttggtgt cgaggatcag gttcgactcc
      481 ttcgtgtatt gcaagaaggg acgttcgagc gggtaggagg caccaaaacc cttcgatcga
      541 gtttccgtct cgtggctgcg acgaaccgcg atctcgcggc cctcgtccga gaggcaaagt
      601 ttcgcgaaga cctgtattac cgtctcaatg tctttcccgt tgtcgttccg cctttgcggg
      661 accggctgga agatattccc aaactcgcct tccacttttt ggaccagtac gcacgtcggg
      721 ccggcaagga atatgaagga atcggagagg ctgatatgga tcggctgctc gaatatgctt
      781 ggccgggcaa tgtgcgggaa ctcgagcatg tgatcgagcg agcggtcgtg ttatcggacc
      841 ctccgatgct caaaatacca acgttggcaa cgtcccggga accgtcgtcc agccggactc
      901 ccaaagagtt tcgccgtctc gaagaagtgg agcgtcagta catccgagag gtactcaacc
      961 acgtgggggg aaagataacg ggaaaagcag gtgcggcgga aatccttggt atgaaaccgt
     1021 ctaccttgca gtttcggatt gataaactag ggcttcggga agcgttggat caatcgagat
     1081 accaaggtaa ggcagctcgt cgaaaatcgt agttcgtacg gatgaaccgg aatcgtcaca
     1141 aggcagttca agcatcaaac agcgagcagc aacgtctttc gacaggggtg ctgaagccgt
     1201 tgtaacgcct gattctcgag ttgtcgaatg cgttcggtcg tcaaccgaaa cacgttggcg
     1261 gtgtcctctc gactcaattc ttcgcccgat tctaacccat agcgaagggt aagaacacgt
     1321 ttttcacggt tgtcgaggga ggctacctgc gcatgaagca cctgaacacg ctgccaatcg
     1381 cctatttgtt gctcgacgct gggcatgggg tctgcgatat cgtggtcatc gatggatacg
     1441 gaatcgccaa tgccgcgttt cacttcccga agccgttcga cagacatcgc tgccgattga
     1501 gcgatatgtt gttcactggc gggatcgggg tgcttggcgt cgtggcgccg ctgggattgt
     1561 tcggcgcggt acgccgcctc gaggacttgt acgggcaaac gcacacaata gccttgttcc
     1621 gcagtggcac gatgaatacc acgacgtacc cagaaagcgg cgtaggtggc gaatcggacg
     1681 ccttttcgga cttggaaccg gtcggcggct tgcatgagtc cgagattgcc ttcctgaatt
     1741 ctgtctaaaa ggtccaatct tcgggaagga agccggatgg caatccggac ggccagccaa
     1801 aggtggcgac gtaccaactc acgttttgtg gtttggatgg tgcgtcgagc cttggcgata
     1861 cgcgcggcgg cctcggggtg ggtttcggga tggacctgat tggcgagatc ttcgagtcgc
     1921 ttcctgtgaa aacgaatatt ttgtaataat tccgagatgt tatcgttgtt tcgggaggtc
     1981 agggccgaga caagacgtcg aagccttgcg tgcgcgactt catcgttttg ggagtattcc
     2041 aagagctgat ggattggctg ttgtccgtct gccgctgcca gaagaagcga acagacttgt
     2101 cgcgcgaggt ccggaacttc gaggatagcc tggagcatgg cctgctcact gttttcgatt
     2161 cgcagggcca gttgccattc ttcttcacgc gtcaggggag ctatggactg aagttcggcc
     2221 agataagact gaccggcgcg ggaagaagcg agctgggata aggaagcgtt gggaacagag
     2281 gctttgaccg tcacacacac ctctacccgg tcatgccggc tcgtatagag ggaaaggtgt
     2341 tgcggtgtgg tcaagggctc ttcctttgag aagtggttgt gctcgcggta ccaagcaaac
     2401 gctagagcta gcaacgcctg tgccagaatt ctacgaccga ggatttgtct tggagcgcgg
     2461 gaaaagacgc ctgttggaat gggttggggc agcttgaacg ctggtgtgcg tctttctctg
     2521 agaatggatc gatcccactc gcgggcggct ccgatccgcc atgggacagt ggtgttcgaa
     2581 c
//
LOCUS       DAOSOR010000712         2565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_40315_length_2565_cov_4.684861, whole genome shotgun sequence.
ACCESSION   DAOSOR010000712 DAOSOR010000000
VERSION     DAOSOR010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40315_length_2565_cov_4.684861"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1632
                     /locus_tag="PK710_23995"
     CDS             <1..1632
                     /locus_tag="PK710_23995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12864.1"
                     /translation="SREARSWSTADRVLVALLAIVGTFCLLQLLWMKYGRDQGIYAVV
                     ADTVLRGGMPYRDAWDFKPPGIFFVYIIAHLLFGSNEWSIRAVEVMAFLSLVPIMGTL
                     ARRFFGDARVGWVAAVLSIWIQSQLEFWHTAQPESFGGVLALAGLAISTHPSSLDNRG
                     QRWKPWVLGLLAGFFFGASGLMKPHLFGVALVAATHAVWTLRKSGHGWRRQLAAFSTI
                     GFGSVIPIVLCIVWFAARGALSDLHHTLFVFAPCYASTTWNQQQFFHYLYAAAYRVTG
                     GYSAIVGIGMVLTAVWGKRTTREREGLLLIALAALPQVLGIAIQSKFFAYHFGATFPF
                     CALFASVGIWRTWQYAQRWPWLGPTLFALGTVAAADARTATVDLSDSFLQRSFKRTRA
                     MLFESKEQRDRIDGELYTVADVHYGANRRVAQWLEQHTSSQDTVFIWGFEPHIYAASA
                     RRPASRFIYNVPQRVAWENQWARDVLMEDLRKDPPKVIVVEHGDIFPIVTGNHSDSAH
                     ALASFPELEQWMETYRKQDRIQDFDIFVREINATP"
     gene            1669..2091
                     /locus_tag="PK710_24000"
     CDS             1669..2091
                     /locus_tag="PK710_24000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12865.1"
                     /translation="MKSLPIVLSGLLSGCLMMPPPQASTGSTNPSQNNAPTEVTPEEP
                     ASPTPSATAPVPSGPSVVSVKLRNTCGNTVKLFFGSKPGFSSGRFDSLGGNTSTNMQL
                     KPGDMVWLTDENRKEISSVTVAESTREIETTCDGIRQK"
BASE COUNT          510 a          702 c          748 g          605 t
ORIGIN      
        1 tctcgtgaag cacgatcgtg gtcgaccgcg gatcgtgttt tggtggcgct gctcgcgatc
       61 gtcggcacgt tttgtttgct tcaacttttg tggatgaagt acggccgcga ccagggcatt
      121 tacgcggtgg tggccgatac ggtgttgcgt gggggcatgc cttatcgtga tgcgtgggac
      181 ttcaagcccc cgggtatttt cttcgtatac atcatcgccc atctgctttt cggatcgaac
      241 gaatggtcca tccgcgcggt cgaggtgatg gcgtttctgt ctttggttcc catcatgggg
      301 acgcttgcgc ggcgattttt tggggatgcg cgcgtgggct gggtggccgc ggtgctgtcg
      361 atatggattc aatcgcaact cgagttttgg catacggcgc aaccggagtc ctttgggggg
      421 gtgttggcgt tggcggggct tgcgataagc acccatccct cctcgctcga caaccgggga
      481 caacgttgga aaccttgggt tcttgggttg ttggcgggat ttttcttcgg tgcctcgggt
      541 ctcatgaagc cacatctatt tggcgttgcc ctcgtcgccg ccacgcacgc ggtctggact
      601 ctacgcaagt cgggtcatgg ttggcgccga caactcgcgg ccttttcaac catcggtttc
      661 ggcagtgtca tacccattgt tctgtgcatc gtttggtttg ccgcgcgagg cgccttgagc
      721 gatttgcatc acaccttatt tgtatttgct ccgtgttacg cttcgaccac gtggaatcaa
      781 cagcagtttt tccattatct ttacgctgcc gcttaccgtg tcaccggtgg atattcggcc
      841 attgtcggca taggaatggt gctgacggcc gtgtggggaa agcgaacaac tcgggaacgc
      901 gaagggttat tgttgatcgc tttggccgcg ttgccacaag tccttggcat cgccattcag
      961 tcgaagttct ttgcgtatca cttcggggcc acctttccct tttgcgcctt gtttgcgtcg
     1021 gtggggattt ggaggacgtg gcaatacgcg caacggtggc cgtggctggg tcccacgctt
     1081 ttcgcacttg gcaccgtggc cgccgccgac gctcgcaccg ctaccgtcga tctatccgac
     1141 tcgtttttgc aacgaagctt caaacgcacc cgcgccatgc ttttcgaaag caaggagcaa
     1201 cgcgatcgca tcgacgggga gctatacacc gtggcagacg tccactacgg ggccaatcgg
     1261 cgcgtggctc agtggctcga gcaacatacg tcgtcgcagg acacggtgtt catctggggc
     1321 ttcgaacccc atatctacgc agcctctgca cggcgtccgg cttccaggtt catctacaac
     1381 gtgccgcagc gggtggcctg ggaaaaccaa tgggctcgag acgtattgat ggaggatttg
     1441 cggaaggacc cgcccaaagt cattgtggtg gaacacggtg atattttccc gatcgtgacc
     1501 ggtaatcaca gcgattcggc tcatgcgttg gcgtcttttc ccgaactcga acaatggatg
     1561 gaaacctatc ggaaacaaga taggatccag gacttcgata tcttcgttcg tgaaataaat
     1621 gctacgccat aaccttcgga tgcgaactgg ccctggagga acttccccat gaaaagccta
     1681 cccatcgtgc tgagtggtct gttgtccgga tgcttgatga tgccaccgcc ccaagcaagc
     1741 acgggctcca ccaacccatc gcaaaacaat gcacccaccg aagtcacccc cgaggagcct
     1801 gcctctccca cgccgtctgc aacggccccc gtaccttctg gaccgagtgt cgtttccgtc
     1861 aagcttcgca atacgtgcgg caatactgtg aagctatttt tcggaagcaa accgggtttc
     1921 agcagcggcc gtttcgattc gttgggaggc aacacctcta cgaacatgca gctcaaacct
     1981 ggagacatgg tttggctgac ggacgaaaac cgaaaagaga tctcgtcagt aacggtcgcg
     2041 gaatcaacca gggaaatcga aacgacctgc gacgggattc gacagaaata ggattcgatg
     2101 gatggcaaaa tcacccctca ccacggggtt cgcgccccgt cgccaccgct tggggaaaga
     2161 ccaaaataat ctataaatac acatagttat tcattgctcg acacatgcct cctcgaagag
     2221 ctggtttgct ctttggtcct ggaacgggca attggggaat gggggggtga gatcaaagaa
     2281 tgctggacga cagccatgag aaggggtttg gtggtgtggg ccatgatagg cgttccgacc
     2341 cgattttttg ggattcacac cacggccatg gcacctgata tcatcgagtg tgcagaacgc
     2401 cccccaggga atgaagcgga tcgaaacgtg ggcgagcggg agagttgttg atggaaagca
     2461 acgcattttg cccgcattgt ggcactccca ttcccctgtc ggccagcgaa tgcccatcct
     2521 gtcaggaacc ggtgcctgcc gccaagacgg tgattggcta cgccg
//
LOCUS       DAOSOR010000713         2551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_40605_length_2551_cov_4.897837, whole genome shotgun sequence.
ACCESSION   DAOSOR010000713 DAOSOR010000000
VERSION     DAOSOR010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40605_length_2551_cov_4.897837"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /gene="nrfD"
                     /locus_tag="PK710_24005"
     CDS             complement(<1..1112)
                     /gene="nrfD"
                     /locus_tag="PK710_24005"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HOT12866.1"
                     /translation="MFEKVFQGSSRYYAWVGALLVLIGIAAVFYLQQLQTGLGITGLG
                     RDVTWGFYIAQLTFLVGVAASAVMVVLPYYLHNYKAFGKMTVLGEFLAVSACTMCLLF
                     VVVDIGSPQRAMNLFFHPTPNSILFWDAVVINGYLLINALVTRVSLVAERKGEAPPTW
                     IKPVIYLSIPWAVSIHTVTAFLYAGLGARPFWLTAILAPRFLASAFASGPSFLILLLF
                     ALKKFGNYDVGKEPILKLAQIVTYGMLINVFFVAMEMFTAFYSGSHHHTIPFQYLYVG
                     LDEHKALVPWMWTSAVMAIIAIAILVNPKTRNDLKILPWACVLVFASIWIDKGMGMVV
                     TGFIPNPVGHITEYMPTLPEMMISLGIYAFGFLMITV"
     gene            complement(1121..1885)
                     /locus_tag="PK710_24010"
     CDS             complement(1121..1885)
                     /locus_tag="PK710_24010"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOT12867.1"
                     /translation="MRHDRRAFLGNAAAGAMVVTSGAALTSCADTGHYEPGKGGTRWA
                     MVVDLRKCAEHEDCRACTQACHKTHNVPDFEGDPLHEIKWIWKEPKFDHVFPSFENHP
                     LPAHIRKKPVLVLCNHCDNPPCVRVCPTKATWRREDGIVMMDWHRCIGCRYCVVACPY
                     GSRSFNWVNPRPYVKEMTPDFPTRMKGVVEKCTLCEKRLSQNLLPACVEACPHGALAF
                     GNLEDPNSEVAKIVATEFTIRRKPDLGTEPEVYYVV"
     gene            complement(1885..2301)
                     /gene="dsrJ"
                     /locus_tag="PK710_24015"
     CDS             complement(1885..2301)
                     /gene="dsrJ"
                     /locus_tag="PK710_24015"
                     /inference="COORDINATES: protein motif:HMM:NF038038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrJ"
                     /protein_id="HOT12868.1"
                     /translation="MTTNRKEAAMYDAGKIVLGLAVFVGVSAFPIWYNIAAGKGAYAV
                     QLEPPPNGTKQCVAETANGYMRAEHMHILDEWRNMVVRDGKRPKVTVDGVHYDYSLSK
                     TCMNCHSNKEKFCDSCHTPAGVAPACWDCHVAPEDK"
     gene            complement(2267..>2551)
                     /locus_tag="PK710_24020"
     CDS             complement(2267..>2551)
                     /locus_tag="PK710_24020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12869.1"
                     /translation="NMEMRLRGGLPRGNAVSYVRDKHGVNMLACICAIDKATLPPLVE
                     YWAPGVGVCGVHELLGNALIMPGESEERKAGKINLRAEEDDDESEGGGDV"
BASE COUNT          611 a          770 c          660 g          510 t
ORIGIN      
        1 accgtgatca tcaaaaaacc aaaggcgtaa atgccaaggc ttatcatcat ctccggcaac
       61 gtcggcatgt attcggtgat atgccccacc gggttcggga tgaaacccgt cacgaccatg
      121 cccatgccct tgtcaatcca gatggaggca aagacgagaa cacaagccca gggcaggatt
      181 ttcaggtcgt tgcgagtttt ggggttgacg agtatcgcaa tggcgatgat cgccatgacc
      241 gcggatgtcc acatccaagg aacgagcgct ttgtgctcat cgagacccac gtacaaatat
      301 tggaagggaa tcgtgtggtg atggcttccg ctataaaacg cggtgaacat ctccatggcc
      361 acgaaaaaca cgttgatgag catgccgtag gtcacgattt gcgcgagctt gaggataggc
      421 tccttgccca catcgtagtt gccgaatttt ttgagcgcga acagcaacag gatgagaaac
      481 gaaggaccag aggcaaaggc cgaagccaaa aagcgcggcg caaggatggc ggtcagccag
      541 aacgggcgcg cgccaagtcc cgcatacaga aatgccgtga cggtgtgaat gctaaccgcc
      601 caggggatcg ataggtagat aacgggcttg atccaagtgg gaggagcctc cccttttcgt
      661 tccgccacca gcgagacccg cgtgaccaac gcattgatca acaggtaacc gttgatcacc
      721 accgcatccc aaaacaggat cgaattgggt gtgggatgga agaaaaggtt catcgctcgc
      781 tgcggactcc cgatgtccac caccacgaat agaagacaca tcgtacaggc ggagacggca
      841 agaaactcac ccaagaccgt catcttgccg aacgccttgt agttatgcag gtagtacggc
      901 agaaccacca tcaccgccga ggccgccacg cccaccagaa acgtaagctg cgcgatgtaa
      961 aacccccagg tgacgtctct tcccaggccc gttatcccca agcccgtttg aagttgttga
     1021 agataaaaga ccgcggcaat gccaatcaaa acgagcaaag cccccaccca tgcataatac
     1081 cgagagcttc cttggaatac tttctcgaac atgggctacc tcacacgacg taatagacct
     1141 caggctccgt gcccaggtcg ggttttcttc gaatggtaaa ctccgtggcg acgatcttcg
     1201 ccacctcgga gttaggatcc tcgagattgc cgaaagccaa cgcgccatgc gggcaggctt
     1261 cgacgcaagc tggcaataga ttttgcgaaa gacgcttctc acacagcgtg catttctcga
     1321 ccacgccctt catccgtgtg ggaaaatccg gtgtcatttc cttcacatac ggacgagggt
     1381 tgacccagtt gaaactcctc gaaccgtaag gacatgccac cacgcaatac cgacatccaa
     1441 tgcaccggtg ccagtccatc atcacgatgc cgtcttctcg acgccacgtg gccttggtgg
     1501 ggcaaacccg cacgcaaggc ggattgtcgc aatgattgca aagcacgagc acgggctttt
     1561 tgcgaatgtg agcaggaagc ggatgatttt caaacgatgg gaacacgtgg tcgaacttcg
     1621 gctccttcca gatccacttg atctcgtgca aagggtcgcc ttcgaagtcg ggcacgttat
     1681 gcgttttatg acaggcctgg gtacatgccc ggcagtcctc atgctccgca cattttcgca
     1741 ggtcgacaac catcgcccaa cgcgttccac ccttgcccgg ttcgtaatga ccggtgtcgg
     1801 cgcaagacgt cagagcagca ccggatgtga ccaccatggc tccagcggcc gcgttaccca
     1861 aaaacgctct tctgtcgtgt cgcatcactt gtcctccggc gccacgtgac agtcccaaca
     1921 tgcgggcgcc acacccgctg gggtatgaca actgtcgcag aacttttcct tgttcgaatg
     1981 acagttcatg cacgtcttgg acagactgta atcgtagtga accccatcga ccgtcacctt
     2041 cggtcgcttc ccatcgcgaa cgaccatgtt gcgccactca tcaagaatat gcatgtgctc
     2101 ggcgcgcatg tagccattgg ccgtttcagc cacgcattgc ttggtgccat tgggcggagg
     2161 ttcgagttgc accgcatacg cccctttgcc cgcggcaatg ttgtaccaaa tgggaaaggc
     2221 actcacccca acgaaaaccg caaggccaag gacgatcttg cccgcatcat acatcgccgc
     2281 ctccttccga ttcgtcgtca tcctcttccg cgcgaaggtt gatcttgccc gctttgcgct
     2341 cctcactttc gcccggcatg atgagggcgt tgcccaatag ctcatgcaca ccgcagactc
     2401 ccacgcccgg cgcccaatac tcgaccaaag gcggaagagt tgccttgtcg atcgcgcaga
     2461 tgcacgcgag catgttcaca ccgtgcttgt ctcgcacata cgagaccgca ttgccgcgcg
     2521 gcaaaccacc tcgtaacctc atctccatgt t
//
LOCUS       DAOSOR010000714         2551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_40608_length_2551_cov_3.750801, whole genome shotgun sequence.
ACCESSION   DAOSOR010000714 DAOSOR010000000
VERSION     DAOSOR010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40608_length_2551_cov_3.750801"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="PK710_24025"
     CDS             complement(<1..1219)
                     /locus_tag="PK710_24025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12870.1"
                     /translation="MSVRRIVAVCAALLCFGFSHIARAQSDTTVHAVGNIGFGYTDNM
                     FGTPSEPPPGQPGPVGVWMLELSPGLAIIHDSPRAIHTLTYAHPFTIYLGHSDSVQQS
                     DVGAWNGLFTLSPRDELILGLSVERSNTRLGNLHAGAEQTTAASQLTGDNMLLQTRLS
                     QLYSHEFSGQWRMTQNASAGAVIPLLVPTPQPNRYEVTLGAGPDYTTGRNAYGFSGEA
                     TYFFTSEVNEGGLNLESAAQIVMTGVFRWRHDLTETWSTELNGGFAGALRADPVRGGL
                     WGPIALAAVRYASEGYDASLSIERSLGPDLLTARTVMADHVLLAGGVPIDQEHNIILR
                     TGTGYSHSRSLEVAESGFLVVAPFATPKSTDDARLVTTFDTFVLDASIGWYPEDLPFV
                     ELRYQHLEQIGEQS"
     gene            1293..1865
                     /locus_tag="PK710_24030"
     CDS             1293..1865
                     /locus_tag="PK710_24030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12871.1"
                     /translation="MRTLGIERLDPDASPELYAELYRVARRVREAGRAVVGLLPTGSD
                     VAVVPLALELAFALADACEGAVAVVDANTRWPALQQMRSPKDERSKKSGFRTTWLASF
                     LAIMTPIEASEAMALDGLDRLLMHETQGFTHMLVDLTGFERHGEHWGMLDAIDGVLLV
                     GHPGATREDEILRLHNDMPPGKILGVILVG"
     gene            1938..>2551
                     /locus_tag="PK710_24035"
     CDS             1938..>2551
                     /locus_tag="PK710_24035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12872.1"
                     /translation="MLQRLRSKNLHTWVSGYGRDVWQRSLDRGHRGLRHILFAVCDHY
                     EPLWNQPTEQVGERRVAAWQHGYPKLAAEFRDADGRPPQHTFFFPGEEYRPFFFDRLE
                     PLIEGGFGEVELHLHHDDATAESFRHDVLSYLDTYAKRGHLSRSADGRVRYGFIHGNW
                     ALANGRPDGRRCGVDNELPLLFETGCYADFTFPSAPDITQPNIV"
BASE COUNT          490 a          604 c          882 g          575 t
ORIGIN      
        1 gggattgttc gccgatttgt tcgaggtgtt ggtagcgcag ttcgacgaaa gggaggtctt
       61 cagggtacca accgatggac gcatcgagca cgaaggtgtc gaaggtggtc acgagacgag
      121 cgtcatcggt ggatttgggg gtagcgaagg gggcaacgac gaggaatccg gattcggcta
      181 cttcgaggga gcggctatgg gagtagcctg ttccggtgcg gaggatgatg ttgtgttctt
      241 gatcgatggg aacgccgcct gcgagaagga catggtcggc catgaccgtg cgagcggtga
      301 gcaggtcagg gccgaggctt cgttcgatgc tcaagcttgc gtcgtagcct tcgcttgcat
      361 agcgcacggc ggcgagagcg atgggtcccc acaggccgcc tcggacgggg tcagcgcgca
      421 gggctccggc gaatccgccg ttgagttcgg tggaccaggt ttcggtgagg tcgtggcgcc
      481 atcggaagac accggtcatc acgatttgtg cggcggactc gaggttcagg cctccttcat
      541 tgacttcgct cgtgaaaaag taggtggctt cccccgaaaa cccataggcg ttgcgtccgg
      601 tggtgtagtc tgggcctgcg ccgagggtga cttcgtagcg attgggttgc ggagtaggga
      661 cgagcagcgg gatgacggcg ccggcggagg cgttttgggt catgcgccac tgtccggaaa
      721 actcgtggga gtagagttgg gatagacgtg tttgcaggag catattgtcg cccgtgagtt
      781 gagaagcggc ggtggtttgt tcggctccgg cgtgcaagtt gccgagccgg gtattgcttc
      841 gctccacgga caggccaagg atgagttcat cgcggggaga caaggtaaat aaaccgttcc
      901 aagctcctac gtccgattgt tggacggagt cggaatgtcc gagataaata gtgaaagggt
      961 gggcgtaggt gagggtatgt atcgcgcggg ggctatcatg gatgatggcg agccctggtg
     1021 ataattcgag catccagacg cccaccggtc ctggttgtcc ggggggcggt tccgaggggg
     1081 ttccaaacat gttgtccgtg tatccgaacc caatattgcc cacggcatgc acggtcgtgt
     1141 cggattgcgc gcgggctata tggctaaaac caaagcaaag cagcgcggcg cagacggcga
     1201 cgattcgacg gacagacatg acagagcctt atcatgccca tcgacggtta tgatcgtcta
     1261 aaatgcaaga tgtgtctgac atagaagatc ccatgcggac tttgggtatc gagcgtctcg
     1321 atccggatgc ctcgcccgag ttgtacgcgg aattgtaccg ggtagcgcgt cgtgtgcgcg
     1381 aagcgggccg ggcggtcgtt gggcttttgc cgacgggatc ggatgtagcg gtcgtgccgc
     1441 tcgctttgga gttggcgttt gctttggcgg atgcttgtga gggtgcggta gcggtggtgg
     1501 atgcgaatac gcgttggcct gccttgcagc agatgcgctc gcccaaggat gagcgttcga
     1561 aaaaatccgg atttcgtaca acatggttgg cgagcttttt ggcgatcatg acaccgatcg
     1621 aggcgtcgga ggcgatggcg ctcgatgggc tagatcgttt gttgatgcac gaaacccaag
     1681 ggtttacgca tatgttggtg gacctgacgg gattcgaaag gcatggggaa cattggggca
     1741 tgctcgatgc gatcgacggt gtgcttctgg tgggtcatcc gggcgcgacc cgtgaggacg
     1801 agattttgcg gctacacaac gatatgcctc caggaaagat tcttggtgtc attctggtcg
     1861 gatgagccgt gcgacaatcg gcgttggacg cagcatgaac gatcgccttt gtgtagtgga
     1921 tccgatacaa aggaggcatg ctgcaacggc ttcggagcaa gaaccttcac acctgggttt
     1981 cgggatatgg tcgcgacgtt tggcagcgat cgttggaccg aggacatcgg gggttacggc
     2041 atattctttt cgccgtctgt gatcattacg agccgctctg gaatcaaccg acggagcaag
     2101 tgggggagcg tcgtgtggcc gcgtggcagc atggctatcc gaagcttgcc gcggagtttc
     2161 gtgatgcgga tggcaggcct ccccagcaca cgtttttttt cccgggagaa gagtatcgcc
     2221 cctttttttt cgatcggctc gaaccgttga tcgaaggtgg gtttggtgag gtggaacttc
     2281 atcttcacca cgacgatgcg accgcggaaa gctttcgaca cgatgtgctt tcttatttgg
     2341 acacctatgc caagcgaggg catctatcgc gtagtgctga tggccgtgtt cgttatggct
     2401 tcattcacgg taattgggcg cttgccaatg ggcgaccgga tgggcgtcga tgcggtgtgg
     2461 acaacgaact gccgctcttg ttcgaaaccg gttgttatgc ggatttcacc tttccgagcg
     2521 cgccggacat cacgcagccc aatatcgtga a
//
LOCUS       DAOSOR010000715         2548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_40684_length_2548_cov_3.806659, whole genome shotgun sequence.
ACCESSION   DAOSOR010000715 DAOSOR010000000
VERSION     DAOSOR010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40684_length_2548_cov_3.806659"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1369)
                     /locus_tag="PK710_24040"
     CDS             complement(17..1369)
                     /locus_tag="PK710_24040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12873.1"
                     /translation="MTFFSKLSRLLLVATASSPWLACGNDAGQGSPSQDADANHSDGS
                     VDGMTDDGPPFPQDASVDGREDASDGTFLDALHDINMDGLADVGDGNLDVISDVGEET
                     IEDAPWDILTDADSGDEWTPTCYYGDFDQPGVHHVACITVPSLDRWVLDRPELTGESI
                     GPATMTRDGAWLAVTVQPAGAPSRIFLAPTNQQEPPKQLVVAPSIHRHIDQMAFSFDG
                     KWLAFTADFDLEGSLGLYVVTLATGHVRRISPAAQAGRNVTGFAWSMGIPSAAHLAFA
                     GNINTESVHELWSAAVDTMTSPTPIVSLPVLGTQAGVRKDLGWDSQGRILFRSNHENP
                     QGWSLYRVHHDGAAIEKVPGSSLDSTTGVATIGSFGVSPDGANVAFSANSPDAMVFNV
                     YLLSEGKDEARRLSQLPVLKKPGTVSGPSFGDRIAWSADGEWLAVIADWRLASSHWFR
                     "
     gene            1425..2300
                     /locus_tag="PK710_24045"
     CDS             1425..2300
                     /locus_tag="PK710_24045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12874.1"
                     /translation="MRCVVERQDEGDWMSLKLEKAPVQWGDLVFHVLAHVPPARRVAA
                     SVYDKVYVDFVKKHAGPASGRTLAEDAQALGKLAVTHEELASVQQLAWLHETIKSAQA
                     AARWELRELGPEQVDAPEVLSGLVRTAPAAELLRRAALLEEQVWASLPAEVDGRALQQ
                     AFEIVMPTAPWLHRCRVRLVRSLRLRGRVRGNEIWVGMPMVALDLGSEHVAWQAAHEA
                     TVREASVMARKNGMSATYGLVEPVAVVALASRSKRAGLQHEHGNWYGHLARPPSIHPE
                     PLLPEQRHLLTQMLE"
BASE COUNT          541 a          718 c          770 g          519 t
ORIGIN      
        1 acgatggcat cggatatcac ctgaaccaat gcgaagaggc caagcgccaa tccgcaatga
       61 ccgcaagcca ttcgccatcc gcgctccacg caatccgatc gccaaatgaa ggccccgaga
      121 ccgttcctgg tttcttgagt acgggaagtt gcgatagtcg ccgagcttcg tccttccctt
      181 ccgacagcaa gtacacgttg aaaaccatgg catcagggct gttggcgctg aaagcgacgt
      241 tcgcgccatc gggcgacacc ccaaaactgc cgatcgttgc caccccggtt gtcgagtcga
      301 gactgcttcc cggcaccttt tcgatcgccg ctccatcgtg atggacgcga tacagcgacc
      361 agccttgcgg gttctcatga ttggaccgaa atagaatcct tccttgcgag tcccagccaa
      421 ggtcttttcg tacgcccgct tgggtcccca gaaccggcaa cgacacgatc ggagtcggcg
      481 acgtcatggt gtccaccgcc gcgctccaca gctcatgcac gctttccgta ttgatattcc
      541 ctgcgaaagc aagatgggcc gcactcggaa tccccatcga ccaggcaaac cccgtgacgt
      601 ttctgcctgc ttgcgccgcg ggactgatgc gcctgacatg gcctgttgcc agcgtgacga
      661 catacaaacc gagtgaacct tccagatcga aatcagcggt aaaagcaagc catttgccgt
      721 cgaaggagaa ggccatttga tcgatatgcc gatggatgga aggagccacg acgagttgct
      781 ttggcggttc ttgttgattc gtcggtgcga gaaaaatgcg cgacggcgct cccgcgggct
      841 gcacggtcac cgctaaccaa gctccatccc gcgtcatggt ggcgggtccg atggactcac
      901 cggtcagctc cggacgatcg agcacccacc gatccaacga aggaacggtg atgcaagcaa
      961 cgtgatgaac ccccggttga tcaaaatccc cgtaataaca cgtaggagtc cattcatccc
     1021 cggagtcggc gtccgtgaga atatcccatg gcgcgtcttc gatggtttcc tctccaacat
     1081 cggaaatgac atcgaggttg ccgtcgccaa catccgccaa accatccatg ttgatatcgt
     1141 gcaatgcatc cagaaacgtt ccgtccgaag cgtcttcccg accatccacg gaagcatcct
     1201 gcgggaaagg ggggccatca tcggtcatgc cgtcgacaga accatcggaa tggttggcgt
     1261 cggcgtcttg gcttggtgaa ccttgtccag cgtcatttcc gcaagcgagc catggactcg
     1321 atgccgtagc gacaagcaac aagcgcgata atttcgagaa aaatgtcatc gaacctgcat
     1381 agtatagtgg aggaagggaa gaccttcgac acaatcagca tgtcgtgcgt tgcgttgtcg
     1441 agcggcaaga cgaaggggac tggatgtcgc tgaaactcga gaaagcaccg gtgcagtggg
     1501 gcgacttggt ttttcacgtg ctggcgcacg tcccgccggc acgtcgcgtg gcggcctccg
     1561 tctacgacaa agtgtatgtg gatttcgtaa aaaagcacgc gggcccggcc tccggtcgaa
     1621 ccttggccga ggatgcgcag gcccttggca agttggcggt cacccacgaa gagcttgcgt
     1681 cggtgcaaca actcgcctgg cttcatgaga cgataaagag cgcgcaagca gcggctcggt
     1741 gggagttgcg ggaactcggg ccggaacaag tcgatgcgcc cgaagtgctt tcgggtctgg
     1801 tgcgaacggc ccctgcggct gaattgcttc gccgcgcggc gttgttggaa gagcaggtgt
     1861 gggcaagttt gcccgccgag gtggatggtc gtgccttgca gcaggcgttc gagatcgtga
     1921 tgcccacggc tccatggttg catcggtgtc gagtgcgttt ggtgcggtcg ctgcgattgc
     1981 gggggcgggt tcgaggcaac gagatttggg tgggaatgcc gatggtcgca ctcgatttag
     2041 gctcagagca tgtggcgtgg caagcagcgc atgaggccac ggttcgagag gcgagcgtca
     2101 tggctcgaaa aaacggaatg tccgctacgt atggccttgt ggagcctgtt gccgtggttg
     2161 cattggccag ccgttcgaaa cgggctgggt tgcagcacga gcatgggaat tggtatggac
     2221 acctggctcg acccccatcg attcaccccg agccattgct gccggaacaa cggcacctgc
     2281 taacgcaaat gctcgagtga ggttgacttt gaccttgggt tggtccttga catgaacgtg
     2341 aacttgagcc tggaccccaa aaccttgcgt ttgacctggt cttgaaccct agcgattcgg
     2401 tttaggtggt gatgggtacg cctttccggc agtcgataac aatatcgaca ctccgagaaa
     2461 aatgagggga tggctcagac gatatgggat taccggaacc agttttgtgt gccgcgcgcc
     2521 gtgaccgtga ccgtgaccgg tttgcgtg
//
LOCUS       DAOSOR010000716         2520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_41266_length_2520_cov_4.020690, whole genome shotgun sequence.
ACCESSION   DAOSOR010000716 DAOSOR010000000
VERSION     DAOSOR010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41266_length_2520_cov_4.020690"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PK710_24050"
     CDS             <1..216
                     /locus_tag="PK710_24050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12875.1"
                     /translation="SVLDDLVHAAFSRDKVALLTHASRTLTRARTLVRLANRAHLLAE
                     KQRTYFAEQSDEIGKKISGWTRHARTR"
     gene            complement(612..1661)
                     /locus_tag="PK710_24055"
     CDS             complement(612..1661)
                     /locus_tag="PK710_24055"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
                     /protein_id="HOT12876.1"
                     /translation="MKKDGWKGVAMGRLREMVTRAARGKRRSPQVARFLLDEDTELLH
                     LRTELAAQTYEPGPVRPMWIQDPKRRLISVIPFRDRVVQHLLIDTTLQAIERRMAPQS
                     HACRKGHGTHRALREAMGWTRARRWVLKVDIRKFFPSIDHELLRRRLHSVTPVSWRWL
                     SDRFIDAPYEGEQVHLWFPGDTLWTPVERPHGLAIGSLTSQIWANFYVSALDHLLAAG
                     LGIGSFVRYCDDWLVWHDDPQVLRNALAQMQQAAQRLRLRLHPSKTRLYRTTDPVSFV
                     GFVLRRRGDGVIVHLRKDSLRRFRQRVRLMRSLFQAGAIEASEVRSRVQAWLAHARHG
                     HTRRLVEAVMEDIVF"
     gene            1677..2204
                     /locus_tag="PK710_24060"
     CDS             1677..2204
                     /locus_tag="PK710_24060"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOT12877.1"
                     /translation="MLRPALLALLTLVPLVTACESSTQSSPASASAPIRCESGTGFSA
                     PNDKGDLVDVPGSAGKPVVVELWARWCEPCQASVRDLLDHRNDLKDAEVVLLGVLEEG
                     ETIDDARGTLASWGVDSPFLIDRGGALMRRFGFADLPAAVVLDAAGNIRWTSSQKPTL
                     SEIKNAVKAVSEPCP"
     gene            complement(2270..>2520)
                     /locus_tag="PK710_24065"
     CDS             complement(2270..>2520)
                     /locus_tag="PK710_24065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12878.1"
                     /translation="RRYGASWWEERGWRSGYFGAEPPCVDGGSVDPEDEYPFGRPISL
                     DDPLEGGTEGVGAKSVIRHSFRRSTLNGPHHQPSVPLA"
BASE COUNT          503 a          864 c          683 g          470 t
ORIGIN      
        1 tcggtgctcg atgacctggt tcatgccgcg ttcagccgcg acaaggtcgc cttgctcacc
       61 catgcttccc gtacgttgac ccgagcccgc accctcgtgc gccttgccaa ccgcgcccat
      121 ctccttgcgg aaaaacagcg cacctatttc gctgagcaat ccgacgaaat cggaaagaag
      181 atttctggct ggacacgcca tgctcgcacg cgataaactc gccaacgcgt gcggggttcc
      241 gcatgcgcgt cggctgggga agctctaacc gcgtgtgccg cggcggcagt tggaacaaca
      301 atgacccgtc gaggctgcgc ggtgccaacc gcaacaacaa cacgcccgac aatcggaaca
      361 acaacctcgg gtttcgttgc gccagcacca ggaatctccc cgcctttcgg cccaaaacgc
      421 catcaggcga gccagagcag acgcgatgcc ccaacgtcgc gcctggcgtg cttgtcctgg
      481 tccacccgtt gccgacgcgg gtcccccaag acccgatgag catcgttggt ctccgccaac
      541 gatcccccgc tcgccgagtc gcctccacct catacgagcg cgcgcggacg acgggcggga
      601 tttatccatc ctcaaaacac gatgtcctcc atcacagctt cgaccaatcg tcgcgtgtgt
      661 ccatgcctcg catgcgcaag ccacgcttgc acccgcgacc gaacctccga tgcctcgatc
      721 gctcccgcct gaaacagcga gcgcatcaac cgcacccttt gtcgaaatcg acgcaagcta
      781 tctttgcgca gatgcacgat gacgccatcg ccacgacgtc gcaacacgaa tcccacgaag
      841 gatactgggt ccgtggtgcg atacaaccgt gttttcgatg gatgcagtcg caagcgcagg
      901 cgctgtgctg cttgctgcat ctgcgccagc gcgtttcgaa gcacctgcgg atcgtcatgc
      961 cacacgagcc aatcgtcgca gtaacgaacg aagcttccga tgccgagacc tgcggccagc
     1021 agatgatcca aggccgacac ataaaaattc gcccatatct gggaagtcag cgagccgatg
     1081 gcaagtccat ggggccgttc gaccggagtc cataaggtat caccgggaaa ccatagatgt
     1141 acttgctcac cttcataagg cgcatcgatg aatcggtccg aaagccatct ccaactcaca
     1201 ggagtcaccg aatgcagccg acgtcgcagg agttcatggt cgatactcgg aaaaaacttt
     1261 cgaatatcca ccttcaacac ccagcgacgc gcgcgagtcc aacccatggc ttcccgcagg
     1321 gcgcgatggg tcccatgtcc ttttcggcag gcatgggatt ggggtgccat tcgtcgttcg
     1381 atggcttgca gtgtcgtgtc gatgagcaga tgctgcacga cccggtcgcg aaacggaatg
     1441 acgctgatca gccgacgctt cggatcttgg atccacatcg ggcgaacggg gccgggttcg
     1501 taggtttggg ctgccaattc cgttcgtaag tgcagcagtt ccgtgtcctc atcgagcaga
     1561 aagcgcgcaa cctgggggct tcgtcgcttt cctcgggccg cgcgcgtcac catttcccga
     1621 agccgcccca tcgcaacccc tttccacccg tctttcttca tgataccacg cctatcatgc
     1681 ttcgccccgc cctgctcgcc ctactcaccc tggtccccct ggtcacggca tgcgaatcct
     1741 cgacccaatc ctccccggct tcggcttcag ccccgatccg ctgcgaaagc ggcaccggct
     1801 tttccgcacc caatgacaag ggcgacctgg tcgatgtgcc cggttccgca ggaaaacccg
     1861 tggtcgtgga attgtgggca cggtggtgcg agccttgcca agcctcggtt cgcgaccttc
     1921 tcgaccaccg aaacgacctg aaagacgccg aagtcgtctt gctcggggtg ctcgaagagg
     1981 gggaaaccat cgacgacgcc cgcggcaccc tcgcttcctg gggtgtcgac tcgcctttcc
     2041 tcatcgaccg gggcggagcc ctgatgcgaa gattcggctt tgcggacctg cccgctgctg
     2101 tggtgctgga tgccgcaggc aatatccgct ggaccagcag ccaaaaaccg accctcagcg
     2161 agatcaaaaa cgccgtgaaa gccgtgtcgg agccttgccc ctgacccctg cccctcccct
     2221 gccccagccg aagtccgcag ccgatgaagt gatcgagttt acttttgctc tatgccaagg
     2281 gcacggacgg ctggtgatga ggaccgttta aggtgctacg acgaaacgaa tgacgaatga
     2341 ccgacttggc acctacccct tcggttcccc cctccagcgg gtcatccagg ctgatcggac
     2401 gcccaaaagg gtattcgtcc tcggggtcta cgcttccgcc gtccacgcac ggtggctcgg
     2461 ccccgaagta tccgctccgc caaccgcgtt cttcccacca cgaggcccca tacctacgat
//
LOCUS       DAOSOR010000717         2516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_41356_length_2516_cov_3.070297, whole genome shotgun sequence.
ACCESSION   DAOSOR010000717 DAOSOR010000000
VERSION     DAOSOR010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41356_length_2516_cov_3.070297"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1788)
                     /locus_tag="PK710_24070"
     CDS             complement(<1..1788)
                     /locus_tag="PK710_24070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12879.1"
                     /translation="MGLLSVFLLALIFLVSCSSNDSTPEPVMTGPIKEIEYELFPFVV
                     SLDAAAREALLESREDGTLVFDGIPPVLAEVEQGSILVSSVAKNAPYGFLRIVREVEP
                     SNGHLTLRTVGAPPQLAFRKLHLRASDQIASLTSPDNEWYFPTLQPSIKGSGKASEPI
                     DFFLFNGDGDPSTKDDQVHVTGKLSGGLTYDIDIDTSWGKLDAISKVTDCVGKILSFG
                     LGGNCELPEIKAYLHITPNAKADVRFEGAAFKSYEKEYPLATIIVSPGVIEIGPIAFV
                     VLVDVTATIEGRASSRFDVGVSASVDVTTGLDYGSLSGVSFIPPKLNHHFAPEATDVT
                     LSGYAKVSIGPKLSLMLYGVVGPYAELRANASVDADQKRTPCFDVHAGADVGVGFHLQ
                     LPGLGTLVDVGTSIPLVDESVATGSCKQPPNSSSYPPGAGPDADHYLNPTFDPWARQL
                     EGVCSTIPHQTRDMAWIELERAIDGHWALAGSGMDTLTKVDDQGKLVWAKRYLDPAPA
                     DSSKPQALLIQRTLPTKGAGLLLTGHPYAVMEIGQGGGVRWAAKFEPSEKITETGPQG
                     WLSDQRMFMSVADDGQGNAIIAGSYQPDQD"
     gene            1956..>2516
                     /locus_tag="PK710_24075"
     CDS             1956..>2516
                     /locus_tag="PK710_24075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12880.1"
                     /translation="MRIARVSFCTRRLCALLVFLAAGGCAREHAHLVDPASRPMLYHR
                     GNTHGGDRFDASLPSMPWAERASTEDWRADPRPETGLTLEDILSTDSSLLPAPSTENL
                     PVPPRTDAELPPKPVPNLPSLRTMDASTIPDGEACLTQLMGLGVSFRMGPEKKGMKTS
                     VLVDGPIGGVTYRGYQNGPLLCDCRLA"
BASE COUNT          544 a          724 c          696 g          552 t
ORIGIN      
        1 atcctgatcg ggttggtaag atccagcgat gatggcgttt ccctgaccgt cgtcagcgac
       61 cgacatgaac atcctttgat ccgaaagcca gccttgagga cctgtttccg tgattttttc
      121 gctgggttcg aacttcgccg cccagcgcac accgccccct tgcccgatct ccatcaccgc
      181 atacggatgg cccgtgagca acaatcccgc gcctttcgtg gggagcgtac gctgaatcaa
      241 aagagcctgc ggcttgctcg agtccgccgg ggctggatcg aggtaacgtt tcgcccagac
      301 caacttgcct tggtcatcga ctttggtcag cgtatccatt ccggaccccg ccaaagccca
      361 atgcccgtcg atcgcccttt ccaattcgat ccaggccata tctctcgttt gatgggggat
      421 cgtcgaacac acgccttcga gctgtcgagc ccacggatca aacgtcggat tgagataatg
      481 atccgcgtca ggcccagcgc cgggagggta actgctcgaa ttaggcggct gtttgcaact
      541 ccccgtggcg acgctttcgt cgaccaaagg aattgatgtg cccacatcca ccaacgttcc
      601 aagtccaggc agttggaggt gaaatccaac acctacatca gcgcctgcat gaacgtcgaa
      661 acagggcgta cgcttctggt ccgcgtccac cgatgcattc gccctcaatt ccgcgtaggg
      721 ccccaccacg ccatacaaca tcagcgaaag cttcggcccg atcgatacct tggcgtatcc
      781 cgatagggtc acatcggtag cctccggcgc gaagtggtgg ttcagcttgg gaggaatgaa
      841 actgacacca gaaagcgaac cataatcgag ccccgtcgtc acatcgacag aagcagaaac
      901 ccccacgtcg aaccgactcg acgcgcgccc ctcgatggtg gccgtgacat ccacaagcac
      961 cacgaatgcg attgggccta tctcgatgac cccaggcgaa acgatgatgg tcgccagcgg
     1021 atattccttc tcgtaacttt tgaaggccgc gccttcgaaa cgcacgtccg ccttcgcgtt
     1081 cggcgtgata tgaagatacg ctttgatctc cggaagttca caatttcccc caagcccgaa
     1141 agagagtatt ttcccgacgc aatcggtgac tttcgatatg gcgtcgagct tgccccacga
     1201 cgtatcgatg tcaatatcat aggtcaatcc accgctgagt ttgcccgtga catgaacctg
     1261 atcgtctttg gtggatggat ccccatcgcc attgaacaag aaaaaatcga tgggttcact
     1321 ggctttgccg ctccctttga tgctgggttg caatgtcggg aaataccatt cgttgtcagg
     1381 ggaggtgagg gaagcaatct ggtcggaggc tcgaagatga agttttcgga acgcgagttg
     1441 aggcggcgcc cccacggtac gtaaggtgag gtggccgttg gatggttcca cttcacgaac
     1501 gatacgtaaa aaaccatagg gcgcgttttt cgccacgctc gaaacaagga tcgagccctg
     1561 ctctacctcg gccaacacgg gtggaatccc atcgaatacg agtgtcccat cctcccggga
     1621 ttccagcagc gcttctcgtg ccgccgcatc gagggaaacg acgaacggaa aaagttcgta
     1681 ttcaatttcc ttgatgggcc cggtcatgac cggctcgggc gtggaatcgt tggaagaaca
     1741 agataccagg aaaatcaatg ctaggagaaa gactgagagt agacccatga tcctgcctga
     1801 gaatatggag tggcccgcgc ttcaagccta acaaatatgg acattggttg catccgtctt
     1861 gttgattcct ctttttttac cggaaatggg gaagacatcg gagggattga tccttttttt
     1921 tggtccatgg ccgtgatgga tgacaggttc ggggtatgcg aatcgctcgc gtgtcgttct
     1981 gcacccgaag gctgtgcgcg ctgcttgttt ttttggcagc cgggggctgt gctcgcgagc
     2041 atgcccatct cgtcgacccg gcgtcacggc ccatgctcta tcatcgcggt aatacgcacg
     2101 gaggggaccg tttcgatgct tctctgccct cgatgccgtg ggcagaaagg gcttcgaccg
     2161 aggattggcg ggccgacccg cgaccagaaa cgggattgac gctggaagat atcctctcga
     2221 ctgattcttc tcttttaccc gccccgtcca cggaaaacct gcctgtccca cccagaacgg
     2281 atgctgaact accgcccaag cccgtgccga atttgccgtc gcttcgaacg atggatgcca
     2341 gtaccatccc cgatggcgaa gcatgcctga cgcaactcat ggggttgggg gtgtcatttc
     2401 ggatggggcc ggaaaaaaag ggaatgaaga cgtcggtgct cgtcgatggg ccgatcggag
     2461 gcgttacgta tcgcggttat cagaacggcc ctcttctttg tgattgtcgt ctcgcg
//
LOCUS       DAOSOR010000718         2513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_41421_length_2513_cov_4.207893, whole genome shotgun sequence.
ACCESSION   DAOSOR010000718 DAOSOR010000000
VERSION     DAOSOR010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41421_length_2513_cov_4.207893"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PK710_24080"
     CDS             <1..231
                     /locus_tag="PK710_24080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12881.1"
                     /translation="TPHPTVTAKASITARKDPRIRRATVRLRLRRRVQGLDASWEFMA
                     GLNPSTPSRLHPTGSFEINPNPYRGIFIENSR"
     gene            complement(252..977)
                     /locus_tag="PK710_24085"
     CDS             complement(252..977)
                     /locus_tag="PK710_24085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT12882.1"
                     /translation="MTQPLVELRQVKKTFHHLGRPLEVLKGIDLQLHRGELAAIVGPS
                     GAGKSTLLHCLGTLDLPTSGQIMLEGQDITALPGARLSALRNKTIGFVFQFHHLLPEF
                     TALENVQMPGLIHGQSRSETAPRARQLLKDVGLAERATHRPGELSGGEQQRVALARAL
                     FMDPPILLADEPTGNLDSATSDAIHDLFLQVNRERGTTIVVVTHNVGFAERMPRIIRL
                     RDGFVEKDVRRELEKKQAEEALP"
     gene            complement(986..>2513)
                     /locus_tag="PK710_24090"
     CDS             complement(986..>2513)
                     /locus_tag="PK710_24090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT12883.1"
                     /translation="TAISFRLPRPRGTQFVIHHELIRMTALVSSVLFLLGVLALLLPL
                     VLNVLEGRSFISFVGARHVRAAKSGFLTVISVLSISGVAVSSCALVSVSSIMGGFGAD
                     LQRKILGNNPHITIDVQDQGGFDDWEAVLQRVRLVPGVAAATPVVAGEAMGSSHTNTS
                     GTMVRGIDPDSIGNVIDLLHNIEVGKFEYLREPELLTRLPAGEIIGLGPGGEPFRKGP
                     DIGGSLFRGTVTPVDPSVQEVLRAPAIHPGVIVGRELAKTLHVYVGDEITLISPMGDL
                     GPMGVMPRVRKFRVAAIFYSGMYEYDASHAYVLLDVAQDLFSMGTNVSSIDLRAVQGE
                     AAAELRPAVYDSVQRETLRVRDWKELNRNLFSALKLERVATFIILSLAIAVASFCIIC
                     TLLLMVTEKGKEIAVLKSLGASSQAIMRVFMLEGVVIGGIGTVFGVVSGIALTVGLSL
                     YGLRLDPEVYYIDRLPIKVDWVDWVLVAVSALVICTLATIYPAYAASTLRPVESLRHE
                     "
BASE COUNT          629 a          852 c          582 g          450 t
ORIGIN      
        1 acaccccatc ccaccgtcac cgcaaaggcg agcatcaccg caagaaaaga ccccagaatt
       61 cgccgcgcaa ccgttcggct ccgcctgcga cgacgggtgc agggactcga tgcttcgtgg
      121 gaattcatgg caggactcaa cccttccacc ccatccaggc tacaccctac cggctcgttt
      181 gaaatcaacc ccaacccata ccgcggcatc ttcatcgaga attcgagata gatgccccct
      241 cggtggccac ttcacggcaa agcttcctcg gcctgctttt tctccaactc ccttcgcaca
      301 tccttttcca caaaaccatc gcgcaaccga atgatgcgcg gcatccgttc cgcaaacccc
      361 acgttatgcg tcaccaccac aatcgtggtg ccccgctccc gattcacctg caaaaacaaa
      421 tcatgaatgg catcactcgt ggccgaatcc aaattgcccg tcggctcgtc cgcaagcaag
      481 atcggaggat ccatgaacag cgcccgagcc agtgccaccc gctgttgctc gccaccggac
      541 aactcccccg gacgatgggt ggcgcgctct gccaagccaa cgtcttttag caattgcctc
      601 gcacgcggcg ccgtttccga acgactctgc ccatggatga gccccggcat ctgcacgttc
      661 tccaacgccg taaactccgg aagcaaatgg tgaaactgaa agacaaatcc aatcgtcttg
      721 tttcgtaacg ccgaaagccg cgctcccgga agcgcggtga tatcctgccc ttcgagcatg
      781 atttgccccg aggtcggcag gtcgagggtc ccaagacaat gcaacaacgt gctcttgccc
      841 gctccggaag gccccacgat cgcggccaat tcgccccgat gaagttgcaa atcgattccc
      901 ttgagcactt cgagaggacg gcccaagtga tgaaaggtct tcttgacttg acgaagttca
      961 accaggggtt gggtcacggc tttgcctact cgtgtcgaag ggattccacg ggacgaagtg
     1021 ttgatgcggc atacgcggga taaatggtcg cgagcgtaca aatcacaagg gcactgacgg
     1081 ccaccagtac ccaatccacc caatctactt tgatgggcaa ccgatcgatg taatacacct
     1141 cgggatcgag tcgaaggcca tacagcgata atccgacggt caaagcgata ccggagacga
     1201 ctccaaacac ggtgccgata cccccaatca ccactccctc gagcatgaac acccgcatga
     1261 tggcctgact cgatgccccc aaggacttga gaaccgcaat ttcttttcct ttttcggtca
     1321 ccatcagcag caaggtgcag atgatgcaaa agctcgccac ggcgatggca agggaaagaa
     1381 tgatgaacgt ggctacccgc tcgagcttca acgcgctgaa caagttacgg ttgagctcct
     1441 tccaatcacg cacccgaagc gtctcacgct gcaccgaatc atagaccgcc ggtcgaagct
     1501 ccgccgccgc ctccccttga accgcccgca agtcaatcga ggaaacgttc gtacccatcg
     1561 aaaacaaatc ttgcgccacg tcgagaagaa cataggcatg gctggcatcg tactcataca
     1621 tgccactgta aaaaatcgcc gcgacacgga acttgcgcac tctcggcatc acccccatcg
     1681 gccccaaatc tcccatcgga gaaatcaacg taatctcatc tcccacatac acgtgaagcg
     1741 ttttggccaa ctcacgaccc acaatcactc ccggatggat cgcaggagcg cgaagcactt
     1801 cttgaaccga cggatcgacc ggggttaccg tcccacgaaa cagcgatccc ccaatgtccg
     1861 gccccttgcg aaatggctca ccgccaggcc ccaacccgat aatctcccca gccggcagac
     1921 gcgtaagcaa ttccggctcc cgcaagtatt cgaacttgcc cacctcgatg ttgtgcagca
     1981 ggtcaatcac attgcctatc gaatccgggt cgatcccccg caccatcgtt cccgacgtat
     2041 tggtatgcga tgaacccatc gcctctcccg ccaccacggg cgttgccgcc gctacccccg
     2101 gtacgagacg aacccgctgc agcaccgcct cccaatcgtc gaaaccccct tgatcctgca
     2161 catcaatcgt aatgtgcgga ttgttgccaa gaatctttcg ttgcagatcc gcgccaaacc
     2221 ctcccatgat cgaactgacc gacaccaaag cacaggaact caccgcaacg cccgagatcg
     2281 aaagcacgga gatgaccgtc aaaaatccgc ttttcgccgc tcgaacatgc cgcgctccca
     2341 caaacgaaat gaaactgcgt ccctcgagaa cattgaggac caaaggcagc agcaaggcaa
     2401 gaacccccaa taaaaacaat acactagaca ccagcgccgt cattcgaatc agttcgtggt
     2461 gaatcacgaa ctgcgtgccc cgcggcctcg gcaaacgaaa tgaaatcgcc gta
//
LOCUS       DAOSOR010000719         2507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_41547_length_2507_cov_4.071370, whole genome shotgun sequence.
ACCESSION   DAOSOR010000719 DAOSOR010000000
VERSION     DAOSOR010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41547_length_2507_cov_4.071370"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PK710_24095"
     CDS             <1..769
                     /locus_tag="PK710_24095"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOT12884.1"
                     /translation="LLKADNLSIGYGGKALVDRISFQVFAHDFFGLVGPNGAGKSTLL
                     MTLLGSVAPVEGKVHARPSLRIGYVPQRSRMDPLFPMSAIEVVRAGAMGPKAKGKAGW
                     VLSSASQQEAMVALDRVGMKSLGTRPLRDLSGGQQQRVLMARALVREPDLLILDEPTA
                     GMDIPSEVELLDFVSGLRVQGTAILLVVHQISLVAGRANRMALINKDIPLFAVGEANE
                     LLNSETLTALYREPMQVFDAAGQVIVRCGCAGQGARA"
     gene            766..1611
                     /locus_tag="PK710_24100"
     CDS             766..1611
                     /locus_tag="PK710_24100"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOT12885.1"
                     /translation="MSFWGSSFLWLEAVIAACAVGAACAVVGVYMILRRVVFLPAALS
                     QVSGLGVVFAFLLPTLLGLEQGTPWTSPELVAGVVTLAASLLLGWMPEPRRLSRDAVI
                     GVAYVLASALMLIIGDRIPQDSHDINDILFGNAVAVETGQMVVALVVAGVVLSLHGWL
                     MRPFLLVSFDSPTAKAHGVPVRVLDTVLFLTVGLTISVATKTIGAMPVFAFAVLPAAA
                     GLSAFSDMRKVFLFAAGIGAASAFLGYWISFVGSVPTGPCMAVVTALFLLPAVIAGRL
                     WQGKR"
     gene            1667..>2507
                     /locus_tag="PK710_24105"
     CDS             1667..>2507
                     /locus_tag="PK710_24105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12886.1"
                     /translation="MKTRVAATIIAALSVSAVSMAQQTPVPDGEKDNPQKPPSASSED
                     ATRSGAANPGASDASSATGDAALMAELTAAADKDDSSLNASRGSAAIAFEQRQQDPAN
                     YLGKEVIGNETNPSISIILDAAFSAFSKDNRLRFGGHAPTTNGPFIQNAELAASAPID
                     PFFRIDLAHSLSHGHIEEVFLTTTALPWNLQVRAGQFKSQIGRHNPTHLHTWDFVVHP
                     LPNQFLFGGGGLTLPGGEVSVLLPLPWYVEVVGALQIGEAGVFRTKSTDYGAPVMADF
                     VYPA"
BASE COUNT          434 a          645 c          812 g          616 t
ORIGIN      
        1 tctgctaaaa gccgataatt tatccattgg ttacggagga aaagccctgg tcgaccggat
       61 ttccttccag gtctttgccc atgatttttt tggcctcgtg ggtcccaacg gtgcgggcaa
      121 gtccacgctg ttgatgactt tgttggggag cgtcgcgccg gtcgagggaa aggtacatgc
      181 tcgtccttcc ctgcgaattg gctacgtacc ccagcgttcg aggatggacc cgttgtttcc
      241 gatgtcggcg atcgaggtgg ttcgcgcggg ggccatgggg ccgaaagcga aaggaaaggc
      301 tggctgggtg ttgtcatcgg cctcgcaaca ggaggcgatg gtggctctcg accgcgtggg
      361 catgaagtcg ttgggaactc ggcctttgcg ggatttatcg ggaggtcagc agcagcgtgt
      421 gctcatggcg cgggcgcttg tccgagagcc tgacctattg attctcgatg agccgaccgc
      481 gggcatggat attccttccg aggtggagtt gttggatttc gtgagcggcc ttcgagtgca
      541 aggcacggcg attttgttgg tggttcatca gatctcgttg gtggcgggtc gcgcgaatcg
      601 aatggcgctc atcaacaagg atattccgct atttgccgtg ggagaagcca acgaactttt
      661 gaattccgaa acgctcacgg cgctttatcg cgaaccgatg caggtattcg atgccgcggg
      721 acaggtcatc gtgcgttgtg gatgcgcggg gcaaggagca agggcatgag tttctggggc
      781 tcttccttct tgtggctcga agccgtcatt gccgcttgcg ccgtgggagc cgcttgtgcc
      841 gtggtgggcg tgtacatgat tctgcgacgc gtggtttttt tgcccgcagc gctttcccag
      901 gtctcggggc tcggtgtcgt tttcgcgttt ttgctaccca cgctattggg actcgaacag
      961 ggcacgccgt ggacttctcc ggaactggtg gcgggagtcg tgacgttggc tgcttcgcta
     1021 ttgcttggat ggatgcccga gcctcggcga ttgagccgcg atgcggtcat tggtgtggcg
     1081 tatgtgcttg cctcggcgtt gatgctcatc atcggcgatc gaatcccgca ggatagccat
     1141 gacatcaatg acattctttt tggcaatgcg gtggcggtcg aaacagggca gatggtcgtg
     1201 gcgctggtcg tggctggcgt ggtgctcagc ctgcacggct ggctgatgcg gcctttcctt
     1261 ctggtatcgt tcgattcccc cacggccaaa gctcatggtg tgcccgtgcg cgtcttggat
     1321 acggttctat ttctcacggt cggtctaacg atttcggtgg ctaccaaaac gattggagcg
     1381 atgcctgttt ttgcgtttgc ggttctcccc gcggccgcgg gattgagtgc attttcggac
     1441 atgcgaaagg tgtttttgtt cgcggcgggg ataggagcgg cttctgcttt tttggggtat
     1501 tggatttctt tcgtgggctc ggtgccgacg ggaccatgca tggcggtggt tactgccttg
     1561 tttttacttc ccgctgtcat cgctgggcgg ttgtggcaag ggaagcgatg agtgcgggcc
     1621 atgatgggat gaacgtggcg atcatcgatc gcctttggga ggaacgatga aaacacgggt
     1681 tgccgcgacg atcatcgcgg cgttgagtgt gagcgcggtg tcgatggcgc agcagacacc
     1741 cgtgccggat ggtgagaaag acaatccaca aaaaccaccg agcgcctcct cggaggatgc
     1801 gacccggtca ggggcagcga accctggcgc gtccgatgcc agctcggcca ctggggatgc
     1861 cgcattgatg gccgagttga cggcagccgc cgacaaagac gattcttcgt tgaatgcgtc
     1921 tcgcggatcg gcggccatcg cgttcgagca acgacaacaa gacccagcga attatcttgg
     1981 aaaggaggtc attggcaacg aaaccaaccc ttcgatctcg atcatcctcg atgcggcttt
     2041 cagcgcattt tcgaaagaca accggttgcg attcggagga catgctccca cgaccaacgg
     2101 tccgttcatt caaaacgcgg aacttgcggc ttctgcaccc atcgatccat tttttcgcat
     2161 cgaccttgcg catagtctga gtcatgggca tatcgaggaa gtctttttga caaccacggc
     2221 cttgccctgg aacttgcagg ttcgagcggg gcaattcaaa tcacagatcg ggcggcacaa
     2281 tcccacgcat ttgcatactt gggatttcgt ggtgcatccg ttgccgaacc aattcttgtt
     2341 tggaggcgga gggctgaccc tgcctggcgg cgaggtctcg gtattgttgc ccttgccctg
     2401 gtatgtcgag gtcgttggcg ctttgcagat cggcgaagcc ggtgtgtttc gcaccaagtc
     2461 cacggattac ggggcaccgg tcatggcgga cttcgtgtac ccagctc
//
LOCUS       DAOSOR010000720         2502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_41672_length_2502_cov_3.665304, whole genome shotgun sequence.
ACCESSION   DAOSOR010000720 DAOSOR010000000
VERSION     DAOSOR010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41672_length_2502_cov_3.665304"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /gene="trpS"
                     /locus_tag="PK710_24110"
     CDS             <1..816
                     /gene="trpS"
                     /locus_tag="PK710_24110"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOT12887.1"
                     /translation="MAAALLACGVDPTRSILFHQSAVTAHLRLAWIFECVARIGWLNR
                     MTQFKDKVGKNRDSASAGLYTYPVLMAADVLAYKATHVPVGDDQRQHLELANDIAQKF
                     NHDYGVEFFPPIVPMVLGPAARVMSLRDGTKKMSKSDSSDQSRINLTDDADTIAAKIR
                     RAKTDSEPLPSEPEGLENRPEARNLVGIYAALAECEPKEVLTQHGGSGFGAFKEALAE
                     LVIDKLAPIAESMRQHLADPSQIDDQLRQGAVRAAALADPIVTEAEKLVGFLR"
     gene            complement(823..>2502)
                     /locus_tag="PK710_24115"
     CDS             complement(823..>2502)
                     /locus_tag="PK710_24115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12888.1"
                     /translation="GMGMGFASSAGKRASAWVVPLVVMMLGCGSSSEQGASPVTGPDE
                     GTGGNGAAGQGHHEGTGGAAANGSGGSSTGGAGAHGAVGGSGAEAGSGANGGQTGSPF
                     TYGLNLGYYNPQLNDSEESQLGLAAGANSHRHKLTEPFLDRWGDDIHVQELSGMLQAG
                     EQDLVCFLVGASEAHSTAPAGSADWQREHYSPNNLYEPIFTDNGQVNPNNPWAAFVAR
                     VVTTYSPYIHTWEVWNEPDQVGGHWQATSSWDTQPPNPSDLIWWNDTIFAYIRLLRVT
                     WEVVHHLDPQGKVALGGIGYPSFLNAILRYTDEPTSGKVDADHPRKGDAYFDVVSFHY
                     YPIFSNGNSDAGAKGLLHLYDQLQTQLEKAGVSGKTFIVTESGAPRFAVDGRPGGKDY
                     AANYLMKAMALAQNAGIDRIDWFILGDSADPGASSSPFDYMGLYENLMNISELGQAIL
                     TDTGVAYATLGGLLAGAHSDPVATQALGSPSSVALRTKQGDRAYVVWAETTAGEGASA
                     TISVPASHDVTVYRWDYSKTKKTQTLSPNGGAVVVDISSAPSILITPMPVP"
BASE COUNT          555 a          737 c          708 g          502 t
ORIGIN      
        1 atggccgctg cgctgctcgc ttgtggcgtc gatcccacac gttcgatttt gttccatcaa
       61 tccgccgtga ccgcgcattt gcggttggcg tggatattcg aatgtgtggc ccgtatcggc
      121 tggctcaatc gaatgacgca attcaaagat aaagtaggga aaaaccgcga tagcgcttcc
      181 gcgggtctgt atacctaccc ggtgctgatg gccgcggatg tgttggcata caaagccacc
      241 cacgtacccg ttggcgatga ccagcggcaa catctcgaat tggccaacga tatcgcccaa
      301 aagttcaacc atgattacgg cgtcgagttt ttcccaccca tcgtgcccat ggtcctgggc
      361 cccgccgcac gggtcatgag cttgcgcgat ggcacaaaaa aaatgagtaa atccgactcc
      421 tcggaccaat cacgcatcaa tctcaccgac gacgcggaca cgattgcagc caaaattcgt
      481 cgggcaaaaa ccgactcgga acctttgccc tccgagcccg agggattgga aaaccggccg
      541 gaagcaagga atctcgtggg gatctatgcc gcgctggccg aatgcgagcc aaaggaggtt
      601 ttgacccaac atgggggcag cgggtttggc gctttcaaag aagcgctggc cgaattggtc
      661 atcgataaac tcgccccgat tgccgaaagc atgcgacaac acctcgccga tccatcgcaa
      721 atcgacgatc aattgcgtca aggggccgtc cgagccgccg ccctggccga ccctatcgtg
      781 acagaagcag agaaattagt tggattcctg cgatgagcag gctcatggga caggcatcgg
      841 cgtgatcaga atcgaaggag ccgacgaaat gtccaccacc acggcacccc cgttgggcga
      901 cagggtctgc gtctttttcg ttttcgaata atcccagcga tacacagtca catcgtggga
      961 agcaggtacg ctgatggtgg cgcttgctcc ctcgcctgcc gtggtttcag cccatacgac
     1021 ataggcgcgg tctccctgtt tggtacgcaa tgccaccgag cttggagaac caagcgcttg
     1081 ggtagcgacg ggatcggagt gggcaccggc cagcaaaccg cccaacgtgg cataagcgac
     1141 tccggtatcg gtcaggatag cttgccccag ctcactgata ttcatgagat tttcgtataa
     1201 gcccatataa tcgaacgggc tggaagaggc tcccggatcc gcggaatcgc ccaaaataaa
     1261 ccaatcgata cgatcgattc ccgcgttttg cgccagggcc atggctttca tcaagtaatt
     1321 tgcggcgtaa tccttgccgc cagggcggcc atcgacggca aaccggggtg ctcccgactc
     1381 ggttacgata aacgttttac cagatacccc cgccttttcg agttgcgttt gtagctggtc
     1441 gtacaagtga aggagacctt tcgcgcccgc atcggagttg ccgttggaga agataggata
     1501 atagtgaaag ctgacgacat cgaagtaggc atcgcctttg cggggatggt ccgcatccac
     1561 cttgccgctg gtcggctcgt cggtataacg taagatggca ttgagaaacg agggataccc
     1621 aatccctccg agggccactt tcccttgggg atcgagatgg tggacaacct cccatgtaac
     1681 tctaagcaag cgaatatacg cgaaaattgt atcgttccac cagatgagat ccgagggatt
     1741 cggaggctgc gtatcccacg atgacgtggc ttgccaatgg ccccccactt gatccggttc
     1801 gttccatact tcccaggtgt ggatataagg actgtaggtg gtgaccaccc gagcgacgaa
     1861 agcagcccac ggattgttcg gattcacttg tccgttgtcg gtgaagatgg gctcatagag
     1921 attgttggga gaataatgct cccgttgcca atccgcggac ccggcgggag cggtcgaatg
     1981 agcttccgac gcgccgacga ggaagcaaac gagatcctgt tctccggctt gcagcatacc
     2041 ggagagttct tggacgtgga tatcgtcacc ccatcggtcg agaaatggct cggtgagttt
     2101 gtggcgatgg gagttggctc cggcggcgag accgagttgc gattcctcgc tatcattgag
     2161 ttggggattg taatagccaa ggttcaaccc ataggtgaat ggcgagcctg tttgcccacc
     2221 gttggctccg gagcccgcct cggcccccga accacccacc gccccatgag cgccggcccc
     2281 cccggtagac gatccgccgg aaccattggc cgcggcacca cccgtgcctt cgtgatggcc
     2341 ctgcccggcc gcaccgttgc ccccagtccc ctcgtcggga cccgtaaccg gagaggctcc
     2401 ttgctcgctc gaactgccac agccgagcat catcaccaca agcggaacca cccaagcgct
     2461 cgcacgttta cctgcggagg aagcgaaacc catacccata cc
//
LOCUS       DAOSOR010000721         2498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_41758_length_2498_cov_4.529267, whole genome shotgun sequence.
ACCESSION   DAOSOR010000721 DAOSOR010000000
VERSION     DAOSOR010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41758_length_2498_cov_4.529267"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="PK710_24120"
     CDS             complement(<1..851)
                     /locus_tag="PK710_24120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12889.1"
                     /translation="MRFAWTLSIVIVWGCSGPAFSSSESTPAFDAGADTPIDSPTCTP
                     ITCAQLNAACGIIADGCGSTLQCGSCPPNHLCVSDGASFFCQCQPKYCAQLEAHCGLQ
                     DDGCGDTIDCGGCDAPSVCAGKTCQCSKIDCKSAGFDCGLIPDGCGGTIDCGTCEGGE
                     TCGANDQANVCGEGSCTAKTCAQLGVECGDVSDGCGNILSCGSCTPPDTCGGGGIQKK
                     CGCSPTTCGQLGFNCGVVSDGCKGTLDCGTCTPPNTCGANGQANVCGCKPTTCEKEMF
                     ECGNAPDG"
     gene            complement(967..2247)
                     /gene="thrC"
                     /locus_tag="PK710_24125"
     CDS             complement(967..2247)
                     /gene="thrC"
                     /locus_tag="PK710_24125"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOT12890.1"
                     /translation="MNDVPWRLVCTDCGTSYPGLESRYRCDCFGTLDVNHDFRRFRLP
                     SLSVLDARLASRTPIDRSGVWRFREFILPLPTEHIVTRREGNTNLYDAPSVARYTGVE
                     QLFLKHEGENPTGSFVDRGMSVAMSVARQLGATRVACASTGNTSASMASYAAVAGMRA
                     FAFVPEGQITDAKLSQTLAFGAKVIRVAGTFDTIMTLVEDFCESERITLVNALNPFRI
                     EGQKAIGWELMQDLDWEVPDWLVLPGGNLGNASAIYKSLQEMYDLGLLHRLPRFAVIQ
                     PEGANSLYKAFLTADSLVPIPTPTTLLSAIRIGNPVCWKKGLRAVHATDGVVEEVSDQ
                     ETFDAKAIVDKAGIGAESTACTTIAGVKKLVDGGVIESHERVCCILTGHILKEHDSVL
                     AYHHATMPNITAHFANTPILADASIQAIKRATDS"
     gene            complement(2240..>2498)
                     /locus_tag="PK710_24130"
     CDS             complement(2240..>2498)
                     /locus_tag="PK710_24130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12891.1"
                     /translation="PNPRTRNQTRLERPKPKAFQHGPIVSGQLRPCRHYLPNPLISCV
                     LSKPVGQDTCPDDSVIASAFNGRLSISIKPSPTIQEQDNHE"
BASE COUNT          543 a          607 c          801 g          547 t
ORIGIN      
        1 ccatcggggg cattgccgca ctcgaacatt tctttttcgc acgtggttgg cttgcagcca
       61 catacgttag cttgaccgtt tgctccgcat gtattggggg gggtacacgt gccgcaatcg
      121 agggttcctt tgcaaccatc agaaaccacc ccacagttga atccgagctg gccgcaggtg
      181 gttggcgagc agccgcactt tttttggatg cccccgcctc cgcatgtatc aggaggtgtg
      241 catgaaccgc aagagaggat attgccgcat ccgtcggaaa cgtcgccgca ttcaacacca
      301 agttgcgcgc aggttttggc ggtacatgaa ccttcaccgc aaacattggc ttgatcgttg
      361 gcgccacacg tctcgccccc ctcacaggtg ccgcaatcga tggttccgcc gcatccatcc
      421 ggaataaggc cgcaatcgaa ccccgcggat ttacagtcta tttttgaaca ttggcaggtc
      481 tttccggcac acacggaggg ggcatcgcaa ccaccgcaat cgatcgtatc gccgcaacca
      541 tcgtcttgca gtccgcaatg cgcttcgagt tgtgcgcaat acttgggttg gcattggcag
      601 aaaaacgaag ccccatctga gacacaaaga tgattgggcg ggcatgagcc gcactgcaga
      661 gtgctgccgc agccatccgc aatgataccg caagcagcgt tcaattgtgc gcacgtgatg
      721 ggggtacacg taggggagtc gatgggtgta tccgcccctg catcgaaggc aggggtggat
      781 tcggaagaag agaacgctgg gcctgaacac ccccaaacga tgacgatgga aagagtccac
      841 gcaaaccgca tgggtttaag catagcatcg atgatgaggg agggtggacc gttgggggga
      901 gaagtgggac gaaataggat ggggaatatg tgcgccacgt aacgaggtgc ccatgaatgg
      961 agaatgctaa ctgtcggtgg cacgttttat ggcctgtatc gacgcatcgg ccaggatggg
     1021 agtattggcg aagtgagccg tgatgttggg catggtagcg tgatggtatg cgagcacgga
     1081 gtcgtgttct ttgaggatat ggcccgtgag gatgcagcac acgcgttcgt ggctttcgat
     1141 gacaccgcca tcgacgagtt ttttgacgcc ggcgatggtt gtgcaggcgg tagattcggc
     1201 gccgatgcct gctttgtcga cgatggcttt ggcatcgaag gtttcttggt cggagacttc
     1261 ttccacgacg ccgtcggtgg cgtgaactgc tcgcaatcct tttttccaac acaccggatt
     1321 tccgatacga atggcggaca ataaggtggt tggggttggg ataggcacga gggaatcggc
     1381 ggtgaggaag gctttgtaca gactgttggc gccttcgggt tggatgactg cgaagcgggg
     1441 cagccggtga agtagtccca agtcatacat ttcttggagg gatttgtaga tagcggaggc
     1501 attgccgagg ttaccgcctg gcagcacgag ccaatcgggg acttcccaat ccaagtcttg
     1561 catcaattcc catccgattg ctttttgtcc ttcgatacga aatggattca gagcgttgac
     1621 caaggtgatt cgttcacttt cgcagaagtc ttctaccaag gtcatgatgg tatcgaaggt
     1681 tccggcgact cgaatgactt tggctccgaa agcgagggtt tggcttagtt tggcatcggt
     1741 gatctgaccc tcggggacga aagcgaaggc gcgcatgcca gccacggccg cataggaggc
     1801 catggaagcg gatgtatttc ccgtagaggc gcaggccaca cgcgtggcac cgagctgacg
     1861 agcgacactc atggcgacgg acatgccgcg gtcgacgaaa ctacccgttg gattttcccc
     1921 ttcgtgtttg aggaaaagct gctcgactcc cgtgtaacgc gcgacgcttg gcgcgtcata
     1981 caggttggta tttccttctc gtcgtgtgac gatatgctct gtgggaagag gaaggatgaa
     2041 ttcgcgaaag cgccatacac cggagcggtc gatgggggta cgggaggcga gtcgcgcatc
     2101 gagaacgctc agggatggga ggcggaagcg gcgaaaatcg tggttgacgt cgagggtacc
     2161 gaaacaatcg caacggtagc gtgattcgag gcctgggtag gaagtaccac aatccgtaca
     2221 aaccagcctc caggggacat cattcatggt tatcttgctc ctggattgtc ggagagggtt
     2281 tgatggaaat cgacaaacga ccgttgaaag ccgaggctat cacggagtcg tcggggcagg
     2341 tatcttggcc tacgggtttg ctcaatacgc atgaaatgag gggattgggc agatagtgac
     2401 ggcagggccg aagctgaccg gaaacgatgg ggccgtgttg gaaggccttg ggttttgggc
     2461 gttcgaggcg cgtttgattt cttgtgcgag gattgggg
//
LOCUS       DAOSOR010000722         2496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_41804_length_2496_cov_3.854158, whole genome shotgun sequence.
ACCESSION   DAOSOR010000722 DAOSOR010000000
VERSION     DAOSOR010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41804_length_2496_cov_3.854158"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2072)
                     /locus_tag="PK710_24135"
     CDS             complement(<1..2072)
                     /locus_tag="PK710_24135"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOT12892.1"
                     /translation="MPAGLSIRWISSVALLASIAASCTSDSPPSEPMSRDCAHAVWAR
                     ASRPGATLGLVGSWNDWEGISVPMHKREDGWYLAYFVLPPGEYGYWIEEDGIARLDDF
                     NPLTTYREQQEVSLLLAEDCSVPKIQVVTAEGRDDGRSRITGVFLTSSSHSPFDPSSL
                     RAQGVGDSSYRIGRANPNTGQFVLEAQGLARGKYTWTVRAKDKDGREAEPATVSFWIQ
                     PQMQSWQDGLLYQIMVDRYLGDGGEYLTSPVTAGSRAGGTLDGVRASIENGYLESLGV
                     TALWLSPVYLNPIEARQSPWDNHFYEGYHGYWPVESQVVDPRIGGEKALYELVETAHE
                     HGLRVLLDIVPNHVYETNRIYVEHQGDGWFHDGPDKCICGDEPCPWSTHIETCWFTSY
                     LPDYQWKNPEVMRHGALESLWWLRTFDLDGVRIDAVPMMPRSATRRIAHALRGSVVSN
                     TELFILGEIYTGGGQGGINQIRYHLGPAGLDSAFDFPLMWSLRETIAHGKGSFADVQR
                     ILEASDLSYEGSGVTMARIIGNHDTTRFLSEAQGDAARDPWKNPPLQPDEPQPYARHR
                     MAMTAMMTLPGLPVMYYGDEVGLAGGGDPDCRRVMPRMDSLNPHQQAVLEHSRRLGRL
                     RRCSRALHRGSLRTLRADKTTYVYVRDIKDGFPVVVAISTASSTQTLSLSSEEIAAGS
                     YVDVLSGEV"
     gene            complement(2389..>2496)
                     /locus_tag="PK710_24140"
     CDS             complement(2389..>2496)
                     /locus_tag="PK710_24140"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT12893.1"
                     /translation="RTVDNFIVKLRRKIEDSPEKPEHILTVYGFGYKLV"
BASE COUNT          575 a          800 c          625 g          496 t
ORIGIN      
        1 acttctcccg acaagacgtc gacataagac ccggccgcga tctcctccga cgaaagagac
       61 agcgtctggg tcgacgaggc tgtcgatatc gcgaccacca ctggaaaacc atctttgata
      121 tcgcgcacat acacgtacgt tgtcttgtcg gctcgcaagg ttcggagaga accgcgatgc
      181 agcgcgcgtg agcatcgacg caaccttcca agccttcgcg aatgctccag caccgcttgt
      241 tgatgtggat tgagactgtc catgcgcggc atcacacgac gacaatccgg gtctccccct
      301 cccgcgagcc caacctcgtc gccataatac atcaccggca gcccaggcag cgtcatcatc
      361 gctgtcatcg ccattcgatg tcttgcgtag ggttgaggtt cgtcaggttg aaggggcgga
      421 tttttccaag gatctcgagc cgcatccccc tgcgcttccg acaaaaaacg cgtggtgtca
      481 tggttgccga tgatgcgcgc catcgtgact cccgaccctt catacgagag gtcggaagcc
      541 tccaggatgc gttgcacgtc cgcaaaactc cctttgccgt gcgcaatggt ttctcgcagc
      601 gaccacatca aaggaaaatc gaaagcgctg tcgagccccg ccggaccgag atgataacga
      661 atctgattga tgcctccttg tcctcccccc gtataaatct caccaagaat gaagagctcc
      721 gtattcgata ccaccgagcc tcgcaaagca tgcgcaatcc ggcgcgtggc cgatcgcggc
      781 atcatcggta ccgcatcgat acgcacccca tccaggtcga acgtgcgtag ccaccacaat
      841 gactccaacg cgccatgacg catgacctcg gggttcttcc actggtagtc cggcagataa
      901 gacgtaaacc aacaggtttc gatatgcgtg ctccagggac acggctcatc gccgcaaata
      961 cacttgtccg ggccatcgtg aaaccaccca tcgccctgat gctccacgta gatcctgttg
     1021 gtctcgtaga cgtggttggg tacgatgtcg agaagcacgc gcagaccatg ctcgtgcgcg
     1081 gtttccacca gttcgtataa cgctttttcc cccccgatac gcggatccac cacctgggac
     1141 tccaccggcc aatagccgtg ataaccctcg taaaaatgat tgtcccacgg actttgacga
     1201 gcctcgatcg ggttgagata gaccggcgaa agccacagcg ccgtcacgcc aagcgattcg
     1261 agatacccat tttcgatcga cgctcgcacg ccatccaggg ttccccccgc gcgactgccc
     1321 gcggtcaccg gtgaggtcag atactccccc ccgtcgccca aataacggtc gaccatgatc
     1381 tggtacaaca agccatcctg ccaagattgc atttgaggct gaatccagaa agatacggtc
     1441 gcgggctccg cttcgcgccc atccttatcc ttggctcgca cggtccatgt gtatttgcca
     1501 cgcgccaacc cttgcgcctc gagaacgaat tgacccgtat tcggattcgc ccgaccgatt
     1561 cgataggatg agtcaccaac cccctgcgcg cgaagactgc ttggatcgaa cggcgaatga
     1621 gacgacgacg tgagaaagac cccggtaatt cgcgagcgac catcatctcg cccctctgct
     1681 gtcaccactt gaattttcgg taccgaacag tcctccgcca acaacaaaga gacttcctgc
     1741 tgctcgcggt acgtcgtcaa tgggttgaag tcgtcgagcc gagcaattcc atcctcctcg
     1801 atccaataac catattcacc agggggaaga acgaagtatg ccagatacca gccatcctct
     1861 cgcttgtgca tcggcacgga aatcccctcc caatcgttcc aacttcccac cagaccaagc
     1921 gtggcacccg gacgagaagc gcgcgcccat acagcgtgtg cgcaatcacg agacatgggc
     1981 tcggaaggag gcgaatcgct ggtacacgat gcagcgatcg aagcaagcag agcgaccgac
     2041 gaaatccagc gaatcgatag tcccgcaggc attacccgat gaaatgtagt tggatacggc
     2101 gctggtgtcg aatggctccg catccttcga aataccaaaa aataagcagg ctacgagccc
     2161 aataggggct tcgcccctcg agccaactcg ttttcacgtt cgtcgatcga cccggaaccc
     2221 tccctagcca acctcatcgt ccaacgctcc cgagccatca cgcaacgttg aaatagcaac
     2281 gtggttggca cgattgctac agctaattcc caatttctct cccccccatc gccaataccg
     2341 cgcaccaggg cccgcggaat ggaaccgtcg tcatgcaaca ccgtgtattt acacgagttt
     2401 atatccaaat ccgtataccg tgaggatgtg ttcgggtttc tcgggggagt cctcgatttt
     2461 tcgacgcagc ttgacgatga agttgtcgac ggtgcg
//
LOCUS       DAOSOR010000723         2494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_41847_length_2494_cov_4.700697, whole genome shotgun sequence.
ACCESSION   DAOSOR010000723 DAOSOR010000000
VERSION     DAOSOR010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41847_length_2494_cov_4.700697"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="PK710_24145"
     CDS             <1..1027
                     /locus_tag="PK710_24145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12894.1"
                     /translation="DSPTVLLEIERRRLLISQPGPTLRLDKLREAASLAAGALGRNPE
                     VMIIEGFDWEAAEPADVQQLRELARDIGAEIWLSVRSHRHVPVTDPHGIPSPVDRFSD
                     LIDVVLTLESVEGRIVLHVLKHHGKTGVDVGLELDVVTMQLVQDPRMHKRSGPRTWER
                     FVLHSGGARGAESAFGETAERYGIREITFTFNGHDNRVRNRGLRYLSDADLQLGDVSL
                     RYVSHRLGREFPATVSVRRIIQSIWHQVRPCQQVFVIGQIQEDGTVRGGTGWGAELAR
                     RWDKELHVFDQDKKTWFRWDGQAWLNATPVIGSPMFAGIGTAHLTDSGRQAIESLFER
                     SFGPGGD"
     gene            1046..2077
                     /locus_tag="PK710_24150"
     CDS             1046..2077
                     /locus_tag="PK710_24150"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT12895.1"
                     /translation="MMRPLIPQGRISGYARALMMGSLRLALGAGVAFFIAGCTCTDSR
                     EKTGVASNASSLAQASASGSVSATPTAQRPPRPTQAAPKPETITALSSDRFKLHVSRW
                     KAKEPSTVSVLLVHRLGGDRSEWTPLIERLLPSDEPMDIVSFDLRGHGESTVDPAKPN
                     RPVHWSSFDLATFEKMDGDVEAVMGLHEKGNKFKWILVGSDLGANLVVRQAHRRSDNI
                     VAVALVSPGASLRGLELYRPFASVLELPNLLLAGTKDTVSDEPVRLLGQMSKTSTRVT
                     WDVSGHGAEALGKERWQMWDELANWIDERVRGTRTMVETEGSASPPASASSGGVGSVG
                     SVEVSKPAP"
     gene            2074..>2494
                     /locus_tag="PK710_24155"
     CDS             2074..>2494
                     /locus_tag="PK710_24155"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOT12896.1"
                     /translation="MSHELGDKGREDFLSEAQEVIDRLSRDLLLLDATTRVGPVDADL
                     VNSVFRGVHTLKGLCGMFNATHLTALSHELENTLDELRLGKTNLTPELLDQLFRAIEI
                     YNRLLAVSRTMGADEPDEELEAFLRDLRRIGGPASVSS"
BASE COUNT          512 a          611 c          816 g          555 t
ORIGIN      
        1 ggattctccc acggtattgc tggaaatcga gcgtcggcgg ttgctgattt cccaaccggg
       61 acccacgctt cgcctggaca agcttcgtga ggccgcatct ttggccgcgg gggctttggg
      121 acgaaatcca gaggtgatga tcatcgaggg cttcgattgg gaagcggcgg aacccgccga
      181 cgttcagcag cttcgagagc tagcccgaga cattggagcg gagatttggt tgagcgttcg
      241 tagccatcgg cacgtgccgg ttaccgatcc tcatggaatc ccttcgcccg tggatcggtt
      301 ttcggacctc atcgacgttg tgttgactct cgagtcggtg gagggccgga ttgtgcttca
      361 tgttctcaaa caccatggca aaacgggtgt ggacgtgggg ttggaactcg atgtggtcac
      421 gatgcaattg gtgcaggacc cgcgaatgca taagcgatcg ggtccacgaa cttgggaacg
      481 attcgtcttg catagcggag gcgcgagagg cgcggaatcc gcatttggtg agactgccga
      541 gcgctacggc atccgggaaa tcacgttcac gttcaatggg cacgacaatc gcgtccgaaa
      601 ccgtggtctt cgttatctat ccgacgccga cctgcagctt ggggacgtga gcttgcgcta
      661 tgtatcccat cgtttgggcc gggagtttcc cgccacggtg tcggtgcgac gaatcattca
      721 aagcatttgg catcaagtgc ggccatgtca gcaagtcttc gtcattggac agattcagga
      781 agacggaacg gttcgtgggg gaacgggttg gggagctgaa ctggctcgtc gctgggacaa
      841 ggaattgcat gtcttcgatc aagacaaaaa aacttggttt cgctgggatg ggcaggcgtg
      901 gctgaacgct acgccggtca tcggttcgcc gatgttcgcg gggattggaa cggcgcactt
      961 gaccgattcc ggacgccagg ccatcgaatc gctatttgag cggtcatttg gcccgggcgg
     1021 agactgaatc gtcgaagagt tcgccatgat gcggcccctt attccccaag gccgcatctc
     1081 gggttacgct cgtgccctca tgatgggaag ccttcgattg gccctcggcg caggggtagc
     1141 ttttttcata gcgggttgta cgtgtacgga ctcgcgcgaa aaaactgggg ttgcaagcaa
     1201 tgcgtcgtcg ctggcgcagg catcggcttc gggatccgta tccgcgacac cgacggcgca
     1261 gcgcccacca cgtccaacac aagcggcacc gaagcccgaa acgatcacgg cgttgtcgtc
     1321 ggatcggttc aagcttcacg tctcccgttg gaaagcaaag gaaccatcga ccgtatcggt
     1381 attgttggtg catcggcttg ggggcgatcg gtccgagtgg actcctttga ttgagcggct
     1441 attaccatcg gacgagccga tggatatcgt gagtttcgat ttacggggcc atggcgaatc
     1501 caccgtggat ccagcgaaac cgaatcggcc agttcattgg agttcgttcg atcttgcgac
     1561 gttcgaaaaa atggatggag acgtggaagc cgtgatgggc ttgcacgaaa aaggtaataa
     1621 attcaaatgg atacttgttg gttcggacct gggggccaac cttgtggtca gacaagccca
     1681 tcgaagaagc gacaatatcg ttgcggtggc gctggtctct ccgggggcat cgctgcgtgg
     1741 gctcgagtta tatcggcctt ttgcttcggt gttggaactg cctaacttgc tgctagcggg
     1801 aacgaaagac acggtttcgg acgagccggt gaggcttttg gggcagatgt caaaaacatc
     1861 gactcgggtg acatgggatg tgagtggcca tggggcggag gcattgggca aggagcgctg
     1921 gcagatgtgg gatgagctgg ccaattggat agacgaacgt gttcgcggca cacgaacgat
     1981 ggtggaaacg gaaggctcgg cgtcccctcc ggcgtctgca tcgtcagggg gagtcggctc
     2041 cgttggcagc gtggaggtat ccaaaccggc accatgagtc acgagttggg agacaagggg
     2101 cgggaggatt ttctgtccga ggcacaggag gtcatcgatc ggctatcgcg ggatctattg
     2161 ttgcttgacg cgacgacgcg ggtgggtccg gtggatgcgg atttggtcaa cagcgtgttt
     2221 cgtggggttc atacgctcaa agggttatgt gggatgttca acgcaacgca tcttacggcg
     2281 ttgtctcatg aactagaaaa cacgttggat gaattgcgtc ttgggaaaac taacctcaca
     2341 ccggagctgc tggaccagct atttcgagcg attgagattt acaatcgttt gcttgcggtg
     2401 agtcgcacga tgggggccga cgagcccgat gaggaactcg aggcttttct aagagattta
     2461 cgtcgaatcg gcggacctgc cagtgtgtca tcct
//
LOCUS       DAOSOR010000724         2489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_41987_length_2489_cov_3.512325, whole genome shotgun sequence.
ACCESSION   DAOSOR010000724 DAOSOR010000000
VERSION     DAOSOR010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41987_length_2489_cov_3.512325"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>862)
                     /locus_tag="PK710_24160"
     CDS             complement(<1..>862)
                     /locus_tag="PK710_24160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12897.1"
                     /translation="IILGDGAAGVAAAHASLRLGVRPLLISLGNGATTLYSGCLDHGV
                     WNLHPRSSSLDERQKEFIDAIDIWCVDPEKTRVATLAGVVRTADAADRALLNLASHKN
                     ETILVPRIDRPNWDADFLARSWSEDLWCREQGLRFESFDASSLDDDRMRFGSDTEIAF
                     HLDSPEGRTKFGEHLSAEVQRHGGGKSVLLGPWLGIDTPVASILTNHIGIQIGEACAG
                     LGSTAGARWEHAANRWLTQQRITRIQDRVQRLEQVDGDWNVHVSQSKRPYQAKAVVVA
                     TGGIAGGGIIL"
     assembly_gap    863..872
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(888..>2489)
                     /locus_tag="PK710_24165"
     CDS             complement(888..>2489)
                     /locus_tag="PK710_24165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HOT12898.1"
                     /translation="TGVLRDCAIRGLSACLFERNDLAFGASGNSSGMIHGGVRYLTSS
                     PKVTRLSCLDSGYIQRIAPHLLFRIPFIMPIHRSIGSRIFLQLVDALFGAYDSFQPLK
                     RGRPHTRLTPEQARILEPGLSGDIVGAVTFDEWGIDGARLCTANAVDAHQRGAHVRTH
                     TTVEGLELLSSSPARMAVRWRDRITNQSGSVSTRVVVNASGAWAPLTASLADMPPSVA
                     AVRPGKGIHVVFDRRLSNYAVVAQAIDNRQIFLEPWQNVSILGTTDTDYYGDLDQVTA
                     TSDEVRYLIEAIRRVFPSIQNARAIHTFAGVRPTLYAYGPIPDKLSREHEIIDHASHG
                     KDGIYSMIGGKLASYRIFAQQMTDIVAARLDCHQPSQTHLLPLPGGDESLDAGELAKI
                     CGIDPVAARRLIYRHGSRARLIAEQIVESKRTPRFICSCEAITEEEIRFVVRHEFVRC
                     FEDLSRRTRLGLGPCAGLHCVLRAGAIVADELGWTPSQGRSQALRLLLSSSFTRAVAL
                     GSAQARQEALLRAAIRAQVGWGQEP"
BASE COUNT          538 a          692 c          750 g          499 t
ORIGIN      
        1 gaggatgatt ccacctcccg cgataccgcc cgtcgcgacc acgacagcct tggcctggta
       61 cgggcgcttc gactgggaaa catgcacatt ccaatcgcca tcgacctgct cgagccgctg
      121 cacgcgatct tggatccgcg tgatccgttg ttgggtcagc caccgattcg cggcatgctc
      181 ccaccgggct ccggcggtac ttccaagccc cgcgcaagct tctccgattt gtatccctat
      241 atggttagtc aggatcgaag caacgggcgt atcgatgccc aaccacggtc ccagcaatac
      301 agattttccg cctccatgcc gctggacctc cgcgctcaga tgctcaccga atttcgtacg
      361 accctccgga ctatcaaggt gaaaggcgat ttccgtatcg gaaccaaaac gcattcgatc
      421 gtcgtcgagt gacgaggcat cgaagctttc gaaacgaaga ccttgctcac ggcaccagag
      481 atcttccgac catgatcggg cgaggaaatc agcatcccaa ttcggtctat cgatacgagg
      541 cacaagaatc gtttcgtttt tgtgagaggc cagattgagc agggcgcggt ccgcggcatc
      601 ggcggttcga acgactccgg cgagagtggc aactcgtgtt ttttcgggat caacacacca
      661 tatatcgatg gcgtcgatga attctttttg tcgttcgtcc agcgatgaac tcctgggatg
      721 caggttccag acgccatggt ccaaacaacc ggaatacagc gtagtggcgc catttccgag
      781 ggaaatgagc agcggccgca cccccaggcg aagcgatgca tgagccgcgg caactcccgc
      841 cgcgccatcg ccaaggatga tcnnnnnnnn nncatcttcg tgatgattca cggttcttgt
      901 ccccatccga cttgtgctcg aatcgcggct ctcaacaatg cttcttgccg tgcctgagcg
      961 cttcccagag cgaccgcgcg cgtaaacgaa gacgaaagca aaaggcgaag ggcctgcgag
     1021 cgcccctgcg atggcgtcca ccccagttca tccgccacga tggcacccgc acgaaggacg
     1081 caatgcagcc ccgcgcatgg tccaagcccc aaacgagtac gtcgagacaa atcctcgaag
     1141 catcgaacga attcatgtcg aacgacgaat cggatttctt cttccgtgat ggcttcgcaa
     1201 gagcagatga agcgaggcgt acgttttgac tcgacgattt gttctgcaat gagacgcgcg
     1261 cgacttccat gccggtagat gagtcgtcgc gctgccaccg ggtcgattcc gcagatcttg
     1321 gcgagttccc cggcatcgag ggattcatcg ccgcctggca aaggcagaag atgtgtttgg
     1381 gagggctgat gacaatcgag gcgagcggct acgatgtcgg tcatctgttg tgcgaagatc
     1441 cggtagcttg caagctttcc accgatcatc gagtagatgc cgtctttgcc atggctggcg
     1501 tgatcgatga tctcgtgttc acgggacaat ttgtcaggaa tgggtccata agcgtacaag
     1561 gtgggtcgca cacccgcgaa ggtgtgaatg gctcgtgcgt tttggatgga cgggaatacg
     1621 cggcgaatcg cctcgatgag atagcggacc tcatcactgg tggcggtcac ctgatcgaga
     1681 tcgccgtagt agtcggtgtc ggtagtgccc aaaatgctga cattttgcca aggttcgaga
     1741 aagatctgcc gattgtcgat ggcctgagcc accacggcgt agttggaaag gcgtcggtcg
     1801 aaaacgacat ggatgccttt tcctggacga acggccgcga cggagggcgg catatccgcg
     1861 agggatgcgg tcaggggggc ccaagcgccg gaagcgttga cgacgacgcg cgtcgatacg
     1921 gagcctgatt gattggtgat tcgatcccgc cagcgaacgg ccatgcgggc tggggaagag
     1981 gaaagcagtt caagcccttc gaccgtggta tgcgtgcgaa cgtgggctcc gcgttggtga
     2041 gcatcgacgg cattggcggt gcatagtcgt gcgccgtcga ttccccattc atcgaaggtg
     2101 acggcaccaa cgatgtcgcc ggacaggcct ggctcgagaa ttctggcttg ctctggtgtg
     2161 aggcgcgtat gggggcgtcc tcgtttgagc ggttgaaagc tgtcataggc gccgaagagg
     2221 gcatccacga gttggagaaa aatccgagag ccgatgctgc gatggatcgg catgataaac
     2281 gggatacgaa agagcaagtg gggggcgatt cgttgaatat agccggaatc taggcatgac
     2341 aatcgggtga ccttcggact ggaagtgagg tagcgaaccc caccgtggat catgccactg
     2401 gagttgccgc tagcgccgaa ggccaggtca tttcgctcga acaaacacgc ggacaaccct
     2461 cggatcgcgc aatctctgag cactcccgt
//
LOCUS       DAOSOR010000725         2487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_42019_length_2487_cov_4.073191, whole genome shotgun sequence.
ACCESSION   DAOSOR010000725 DAOSOR010000000
VERSION     DAOSOR010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42019_length_2487_cov_4.073191"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="PK710_24170"
     CDS             <1..658
                     /locus_tag="PK710_24170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12899.1"
                     /translation="DEKGNSIPIPIGSRIQDARFAFPPSSLVALLDDRDRLFVWNSKT
                     GDVTPIDESVFPGFGFAHASESIMYAKGQTPELAAFRVDLPGGSPLQLTKGDTPVWGF
                     AFSPDDKQVVFVDAPDGFPTLVVMDSQGGKSHRLTNRRIGPDDVRAGAELAPFPDGRR
                     PPLWIGTTVFLENSAGVFAIDRFGSVRVRYDKGRDLHLDVSATRALFRDGARVRQVLP
                     "
     gene            655..2253
                     /locus_tag="PK710_24175"
     CDS             655..2253
                     /locus_tag="PK710_24175"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT12900.1"
                     /translation="MSLFETKKALWTGLLALAAVVFATPGHAVKFRRPFDANIGVNYG
                     FDNNPGGGCRDYACGGVCYDGHSGTDFPLSLGTAVVAAASGRIGATYNGCANYGGLGN
                     TCGGRCGNYVRIDYNDGTSALYCHLQLNSIMVSVGQQVSCGQQIARSASSGNSSGPHL
                     HFGLRVGGANRDPFAGSCSQSTSYWVAQGSYPHPVPSTQCESVCACSPGQTQSEACGN
                     CGSRSRTCGSNCQWGGWSSCTGQGECKAGSVESRDCCDCGSQTRRCSSQCSWEAWSAC
                     DGPDPNGGQEACETGEPGICAEGRVRCLQGCRSCVRLHEPRQETCDDVDEDCDGVIDN
                     DYPQQIGEPPPQMAARLLDASYSQAIEINGQTQAWATFRNEGRATWKAWDVWLVPTST
                     IDGNVSPFFDSHTWPAFDVAATLQEDVAPGETATFTWTIRAGDSRGKIQDTFQLTSSD
                     GSLMRCPSPHVQVDVLVTQTRRAWEPSPSTLPAPSDETSGSDESSGCACRTEPMPKER
                     YGWVGLLLFLIVRIRQRTRQHPKP"
BASE COUNT          507 a          696 c          748 g          536 t
ORIGIN      
        1 cgatgaaaaa ggaaattcga ttcctatccc tatcgggtca cgtattcaag acgcccgatt
       61 tgcctttccc ccctcttctt tggtcgcttt gctcgacgat cgggatcgct tattcgtatg
      121 gaattccaag actggagatg tcacacccat tgatgaatcg gtttttcctg gatttggatt
      181 tgcccatgca tccgaaagca taatgtacgc aaagggacag actcctgagc ttgccgcatt
      241 tcgcgtggac ctccccgggg gatctcccct acaactcacg aaaggagata cacccgtatg
      301 ggggttcgcc ttctcgccag acgacaagca agtcgtgttc gtggatgctc ccgatggctt
      361 tccgacgttg gtggtcatgg attcccaagg gggaaagagc catcgcttga ccaatcgcag
      421 gattggtcct gacgacgttc gtgcgggggc tgagctggct ccctttcccg atgggcgtcg
      481 tccgcccctt tggatcggta cgaccgtatt tttggaaaac tccgctggcg tgttcgcaat
      541 cgatcggttt ggcagcgtac gggtccgata cgacaaagga cgtgacttgc acttggacgt
      601 aagtgctacg cgagctttgt tccgtgatgg cgcgcgcgtg cgacaggtgt tgccatgagc
      661 cttttcgaaa ccaaaaaagc gctatggacg gggctcctcg ccctcgccgc ggtggtattc
      721 gcgaccccag gccatgccgt caagtttcgt cgtccgttcg atgcgaatat tggcgtcaac
      781 tacggcttcg acaacaaccc gggcggaggt tgccgggact acgcgtgtgg cggcgtttgt
      841 tacgacggcc attctggcac ggattttcct ctttctctgg gaaccgccgt cgtggcagcc
      901 gccagcggca gaatcggagc tacctataac gggtgcgcca attacggggg tcttggcaat
      961 acctgcggcg gtcgatgcgg gaactacgtg cgaatcgact ataacgacgg cacctccgcg
     1021 ctgtactgcc atctgcaact gaattcgatc atggtgagcg tggggcaaca ggtatcgtgc
     1081 gggcagcaga tcgcgcgatc cgcgagttcc ggcaacagtt ccgggcctca cctgcatttc
     1141 ggattgcggg tcggaggggc gaatcgagat ccgttcgcgg gcagttgctc tcaatccaca
     1201 tcgtattggg tagcgcaagg ctcgtatccg catcccgtgc caagcacgca atgcgaaagc
     1261 gtctgcgctt gttcaccagg gcaaactcaa tcggaggcat gcggaaattg cggttcgaga
     1321 agtcggactt gcggatccaa ttgtcagtgg ggaggatggt cttcctgcac gggccagggg
     1381 gaatgcaaag cgggatccgt cgagtctcgc gattgttgcg attgcgggtc tcaaacgcgt
     1441 cgatgctcga gtcaatgttc gtgggaagcg tggtccgctt gtgatggccc cgatcccaac
     1501 ggtgggcaag aagcctgcga aaccggggag cccgggattt gtgccgaggg tcgcgtgcgc
     1561 tgcctgcaag gatgccggag ttgcgttcga cttcatgaac cccgccaaga gacctgcgac
     1621 gatgtcgatg aagactgcga tggcgtcatc gacaacgatt atcctcaaca aatcggcgag
     1681 ccaccgccgc aaatggcagc tcgtttgctc gatgcttcct actcgcaagc cattgaaatc
     1741 aatgggcaaa ctcaagcgtg ggcgaccttc cgcaacgaag ggcgggccac ctggaaagca
     1801 tgggatgtct ggctcgtacc cacctcgacg atcgacggaa atgtcagtcc gttttttgat
     1861 tcccacacgt ggcctgcctt cgacgtggcc gccaccctcc aagaagacgt cgcccccggc
     1921 gagacggcaa cgtttacgtg gacgattcgt gctggggatt cgcgggggaa aattcaagac
     1981 actttccaac tcacgtcgtc ggatgggtcg ctgatgcggt gtccatcgcc tcatgttcag
     2041 gttgatgtgt tggtgacgca aacgcgaagg gcatgggaac cttcgccgtc gacgctgccc
     2101 gcgcccagcg atgaaacctc cggctcggac gaaagttcgg gctgcgcctg tcgaacggag
     2161 ccgatgccca aggagcgata cggctgggtt gggctgctgc tgttcttgat cgtgagaatc
     2221 cgtcagcgta ccaggcaaca tccgaaaccg taggggcggg tttcaaacac gcccatagat
     2281 gcgaaagaat ggtgcatagg gtaccgacga atccaagcgc tctatcggcg gcaagatccg
     2341 cgacaatcgc gcaagatggg cgtttgattg cgagaaaccg tctgcgtatt aggccacacc
     2401 ctgacagatt ccctcatttt tcctgaattc gataggtgcg cttgtcaagc accatccgac
     2461 caacaatctc cggacggccg caagcgg
//
LOCUS       DAOSOR010000726         2473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_42348_length_2473_cov_4.313896, whole genome shotgun sequence.
ACCESSION   DAOSOR010000726 DAOSOR010000000
VERSION     DAOSOR010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42348_length_2473_cov_4.313896"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..>2473)
                     /locus_tag="PK710_24180"
     CDS             complement(146..>2473)
                     /locus_tag="PK710_24180"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOT12901.1"
                     /translation="GFAYQMKTSVTADGFWYEGSWGYHFFALDPLMQLARMATKGGYD
                     LLSDAALQSMFRAPIRFAMPDWTLPPFNDSGSADLISNDRFFEMAWQVYQDPIYLEVL
                     GKRTRGRYALYWGEKDLPQSVTSPLSSELFPDAGYAVLRSGKGAEATYIALDYGPHGG
                     WHGHYDKLGFVLYANGATMAVDPGTQSYAAPTHATWDKVTVAHNTVVVDTKSQQEATG
                     NLHRFLGLPGLSIAAADAGNVYGKPSEPTVDLLRTMVLAPEYLVDRFHVQSSDGSSHD
                     IDWILHSPGEIATDLAQKAYDKFPKEHGYQHLSKAKSTETSETWRVAFEQKPSASNIG
                     SVWGSETSVKASFSSSKEQAHEGSWSGKMTYDFQANTGYGLYAMPTLEPVQDKPDTLR
                     FWVYGDGSKHGFGVRVNDATDERFVKKLGSIDWKGWKSFEVSEVESWSHYLGNNDGVL
                     DTPVKNVSFELTYQAGGASAGAIWVDDVHVSFDGGSPEQVTDFEVPYRALRVQMLADP
                     STTVVTGEGLGPNLLVPVPFVMARRHGQDTTFLSLLEPGAEQPRIKDFAALPTDAAPE
                     DEALAYRASTDDFIDTALVLAKGKAGTERTFGDASCDGSSCLIRTKGNELLRLVVGEA
                     TALTQGLPLLVSEAPLPGLQVDYVSENQGMDITAQVQFVTVLRVLGPQVTSVRVNGQD
                     TPFQRDGDYVVLNLEPSEEKPDAGSEPSEDADVSENQDGATDAKENEKGKTDASDGND
                     DGCGCRVVGAPARGGVGGMLALLGLWGVVLRRRYR"
BASE COUNT          506 a          766 c          658 g          543 t
ORIGIN      
        1 cacgatgggg cggttcgaac gcacctgcca gccggcggtt ttcgaaatct gtgaaacgtc
       61 tgtaatatat tgaattaatt gctgattttg tgtccacccc cgtggtgaac ggcagaacat
      121 cccatgccga aagcacggtt gccctctacc gatatcgacg ccgcaatacc acaccccaca
      181 gaccaagcaa cgcaagcatt ccccccacac cgccccgcgc gggtgcgccc acgactctgc
      241 agccgcaacc atcgtcgtta ccgtcgcttg cgtccgtctt gcctttttcg ttctcttttg
      301 cgtctgtggc tccgtcttgg ttctccgaga cgtcggcatc ctcggatggt tccgaacccg
      361 catccggctt ttcttccgaa ggctcgaggt tcaaaaccac atagtcgccg tcgcgttgga
      421 aaggcgtgtc ctggccgttg acgcgcaccg aagtcacctg cggcccaagc acccgaagca
      481 cggttacgaa ttgcacctgg gctgtgatat ccattccctg gttttccgag acataatcca
      541 cttgcaagcc cggcagcggc gcctcggaga caagcaacgg caagccctgg gtcaatgccg
      601 tggcttcccc caccaccaga cgcagcaatt cgttgccctt tgtgcggatg agacacgacg
      661 atccatcaca agacgcatcg ccgaacgttc gttccgtccc cgcctttcct tttgccagca
      721 cgagtgccgt atcgatgaaa tcatcggtgg aagcccggta cgccagggct tcgtcctcgg
      781 gtgccgcatc cgtgggcaat gccgcgaaat ccttgatgcg cggctgctcc gcgcccggtt
      841 ccagcaacga aaggaaggtg gtatcctgcc catgacgcct tgccatgacg aaggggacag
      901 ggactaacaa gttcggtccc agcccttctc ccgtgaccac cgtggtggaa gggtctgcaa
      961 gcatctggac gcgcagggca cgatacggaa cctcgaaatc cgtgacctgc tccggactgc
     1021 ctccgtcgaa agatacgtga acatcatcga cccagatcgc tcccgcgctc gcccctcctg
     1081 cttgataggt caattcgaaa gacacgtttt ttacgggtgt atccaatact ccatcgttat
     1141 ttccaaggta gtgggaccag ctttctacct ccgaaacctc gaaggatttc catcccttcc
     1201 aatcgataga cccgagtttt ttgacgaaac gttcgtccgt ggcgtcgttg acacgtacgc
     1261 cgaatccgtg tttcgagcca tctccgtaga cccaaaagcg cagggtgtca ggcttgtcct
     1321 ggaccggctc gagggtgggc atggcgtaga gcccgtagcc cgtattggcc tggaaatcat
     1381 acgtcatttt tcccgaccag ctcccttcgt gggcctgttc cttgctagac gagaaagaag
     1441 ccttgacgga ggtttcgctg ccccacaccg agccgatgtt ggaagcagaa ggcttttgct
     1501 cgaaagccac ccgccaggtt tcgctggttt ccgtgctttt tgccttggaa aggtgctgat
     1561 agccgtgttc cttgggaaac ttgtcgtagg ctttttgcgc aagatccgtg gctatttcgc
     1621 ctggggaatg caagatccaa tcgatatcat ggctcgaacc atcgctcgat tggacatgga
     1681 atcggtcgac caggtattcc ggggccagga ccatggttcg caacaggtcc accgtaggtt
     1741 ctgacggttt tccatagaca tttcccgcat ccgccgcggc gatggacagg cccggaagcc
     1801 ccaaaaaccg atgtagattg ccggtggctt cttgctgcga tttcgtatcc acgacaaccg
     1861 tgttgtgggc gactgtcact ttatcccagg tggcgtgggt gggcgccgcg tacgattgcg
     1921 tgcccggatc cacagccatg gtcgcgccgt tggcgtaaag tacgaatcca agcttgtcat
     1981 aatgtccgtg ccaccccccg tgaggaccgt agtcgagagc gatatacgtt gcttccgccc
     2041 cttttccaga gcgcaacacc gcatagcccg catcggggaa taattcgctg gacagggggc
     2101 tcgtcacgct ttgcggaagg tctttttcac cccaatacag cgcataacgg cctcgggtcc
     2161 gctttccgag cacttccagg taaatggggt cttggtagac ttgccacgcc atctcgaaaa
     2221 accgatcgtt cgagatgaga tccgcgctcc cagaatcgtt gaacggcggc agcgtccagt
     2281 ccggcatggc aaaacggata ggagcccgga acatggattg aagcgcggca tcgctcaaca
     2341 ggtcgtaacc ccccttggtc gccatccgcg ccaattgcat caacggatcc aacgcaaaaa
     2401 agtggtatcc ccaagatccc tcgtaccaaa acccgtccgc cgtcacgctg gttttcatct
     2461 gataggcaaa ccc
//
LOCUS       DAOSOR010000727         2472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_42378_length_2472_cov_2.658254, whole genome shotgun sequence.
ACCESSION   DAOSOR010000727 DAOSOR010000000
VERSION     DAOSOR010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42378_length_2472_cov_2.658254"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1388)
                     /locus_tag="PK710_24185"
     CDS             complement(150..1388)
                     /locus_tag="PK710_24185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT12902.1"
                     /translation="MKKVKRIIFWTIVALIVLGVGGVIVKSALAEKKVAVYTRAVERK
                     TITQAVTASGKVQPVVKVEISAYVSAEIIRLSVQEGQAVKQGDLLVELDSMRYRATRD
                     GYGAAVTAAQSQGKLAQINWEQAKRDFERVQSLHQQGLASKADVEAAKTKLEAQSSVV
                     RAAQDDVQRASAQLRLAGDDVSKTVLRSPIDGVVIALNKEAGEMVVGSGFTRDVIMTV
                     ADLSTMEVRVEVDENDVPHVKVGQKANITVDAFPKEKFEGEVTSIANSAKVKGAGTQE
                     EITNFEVAIRLVKPARPLRPGMSATAEIVTEVKKDVLAVPIQCLTMRDPEAAPKTPVS
                     LMRSDKLKEVVFSVVDGRASLVVVKTGISSDFDYEVEGPIEPGLPLVCGPFKALNKEL
                     ESGLLLDVKEEASMMSEKEP"
     gene            complement(1708..2091)
                     /locus_tag="PK710_24190"
     CDS             complement(1708..2091)
                     /locus_tag="PK710_24190"
                     /inference="COORDINATES: protein motif:HMM:NF015754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCCL domain-containing protein"
                     /protein_id="HOT12903.1"
                     /translation="MKNLLVICVLMSFAIGLAGCCKNGKSVDCNTKLKDVEGQSGELG
                     TSFSITCPAGCRGGSIWGTDTYTTDSPICLAGIHAGVIAQDKGGDVKVTIVKSLPSYK
                     GSARNGVTTSDWANSWGDTAFQVSK"
BASE COUNT          521 a          801 c          594 g          556 t
ORIGIN      
        1 attcatcaac gtggattttc cagagcccga cggccccatg atcgccacgt attctccttt
       61 ttcgatgtgc agatcgattc ctttcaggac ctcgattttt tgggctccaa gatcgtaggt
      121 cttgcgaata tcgatacaac gaagcaggct catggctctt tctcgctcat catggaggcc
      181 tcttccttga cgtcgagcaa cagtccggat tccaactcct tattcagcgc tttgaaaggc
      241 ccacacacca gcggcaatcc gggctcgatc ggcccttcca cctcgtaatc gaagtccgaa
      301 ctgatcccgg ttttcacgac caccagcgac gcgcgcccgt ccacgaccga gaataccacc
      361 tctttcaact tgtcggatcg catcagcgat accggtgttt tcggcgcagc ctcgggatct
      421 cgcatcgtca ggcattgaat gggtaccgca agaacatcct ttttgacttc ggtcacgatc
      481 tccgcggtgg cgctcatgcc gggccgtagg ggacgcgctg gcttcaccag acgaatcgcc
      541 acctcgaaat tcgtgatttc ctcctgcgtt cccgcccctt tgaccttggc cgaattcgcg
      601 atcgacgtca cttctccctc aaatttctcc ttgggaaacg catccaccgt gatattggcc
      661 ttttgaccga ctttcacgtg gggaacgtca ttttcatcca cctcgactcg cacctccatg
      721 gtggatagat cggccaccgt catgatcacg tcgcgagtaa atcccgaccc taccaccatc
      781 tccccagcct ccttgttgag cgctatgaca accccatcga tgggggagcg aagcacggtc
      841 ttggaaacgt cgtcgccagc caaacgaagc tgggccgagg ctcgctgcac atcgtcctgc
      901 gccgcacgaa ccaccgagga ttgagcctct agcttggttt ttgccgcttc cacatccgct
      961 ttcgaggcca atccttgttg gtgcaggctc tgtacgcgtt cgaaatcgcg cttggcctgc
     1021 tcccaattga tctgcgcaag cttgccctgg gattgtgccg cggtcaccgc cgcgccatac
     1081 ccatcccgag tggcccgata tcgcatggaa tcaagctcca cgagcagatc cccctgtttc
     1141 accgcctgcc cttcttgtac cgacagacgt atgatttccg ccgatacata cgcggatatc
     1201 tcgaccttga ccaccggctg caccttgccg gaagcggtca ccgcctgcgt gatggtcttt
     1261 ctttccaccg ctcgcgtata tacggcgact tttttttcgg ccaacgcgct ttttacgatg
     1321 actcccccca cgccaagcac gatcaacgcc acgatggtcc agaaaatgat cctctttacc
     1381 ttcttcaagg ggccacctcg gtactctcta cggggctgcg atgagactat tacaacagac
     1441 ccggagtttt atttcgtttt tttgggtcga aacacaaccg acaccggagg atgcagggag
     1501 gaagtccgcg cgtcagcgct ccgcaagcac gtggcaagtc aacgaaaata ctagggtttc
     1561 tccaaatatc gtggccgggg aggccagagg tcggagagac aaaaaaaacg caagctcaag
     1621 cgctcgctcc tccacgagca cggacccacg ctcgacgctt cgtcgcggga aaagcgaatc
     1681 gattcgatat ccccccaagg ggcgcgctta cttcgacacc tggaaagcgg tatcccccca
     1741 ggaattggcc caatcgctcg tggtcacgcc gtttcgcgcg cttcccttgt acgagggcaa
     1801 actcttgacg atggtgactt tcacgtcgcc gcccttgtcc tgcgcgatga caccagcgtg
     1861 gattcccgcc aagcaaatgg gagaatccgt ggtgtaggta tccgtccccc atatcgatcc
     1921 accccggcag cccgccggac acgtgatgga aaacgaagtg ccgagctcgc cgctctgccc
     1981 ctccacatcc ttcaacttcg tgttgcagtc cacggatttg ccgtttttgc aacatcccgc
     2041 cagaccgata gcaaacgaca tgagcacgca tatcacgagg aggttcttca tcgtgttcac
     2101 ctgaatgcaa gagtttcttc tcgcatttta tcgggatgtc tcacggcgtc aatcgttact
     2161 tcaaaaatca cggcgatgct tcaaagctcc ccctttcccg gatgaacgct gcaactcatc
     2221 ggtatttcaa ggataatttg cgatctcctc gccgccatgg gcatggatgc tccagtccaa
     2281 ccagcccatc gcctcgctca cgatcacggt caccgccaca gtcacggccc acgagaaacg
     2341 gtcacggtca tcccatatcg ttcaccgcaa aacgccagta caatgttgaa tagcccaggc
     2401 caactgttgg gggtgctcca agggggccaa ggaccgagcc aaactcaaca cccaagaatc
     2461 tttagcccgt at
//
LOCUS       DAOSOR010000728         2463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_42572_length_2463_cov_3.507475, whole genome shotgun sequence.
ACCESSION   DAOSOR010000728 DAOSOR010000000
VERSION     DAOSOR010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42572_length_2463_cov_3.507475"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1511
                     /locus_tag="PK710_24195"
     CDS             <1..1511
                     /locus_tag="PK710_24195"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HOT12904.1"
                     /translation="TVVIEHTTDAVRHPLLHQGLLIAEAAFTLLFTIEYVLRWYASPN
                     RWRYPFTTYALIDLIAILPSFLMFSHVMGIGSEFLMLRTVRLLRTLRFLRLLRLLRIL
                     RHGYTLYRLAVVTRIYASALVYQYRLGRLARLGLISLASWIAGANLVHLIERLSTTAN
                     SQSPYASDYWESYWGVLVFIISGMDAPEPISLGGRIVVTLLLLVGICIVAVFTGEVVS
                     VLVRSEERSGRMALKPPGLKLAEHIVILGRNDHLEKIIQQVHHALAGRHYILVVCQEA
                     EQLPSPGPRVHGRVFALEGNPARDDVLNAANLEDARRVIVLSAADTQASPLERDNIAL
                     MHAIAAVARNKRIPLVLELQEPESLRYASSIQSADCLVSRSFGEMLMSQSVHNKGVAE
                     IYDELMTFSPNTNEFHRLRVPPSLVGSTFRQAQEYFLDLDSEAILLLGIDGPEQQAYQ
                     DFRLCVGNDADNPLRDHVLTASDYLIVCAVERPGFEPHHPEKAWQTHELPRR"
     gene            1535..2284
                     /locus_tag="PK710_24200"
     CDS             1535..2284
                     /locus_tag="PK710_24200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12905.1"
                     /translation="MNEKVPAIVEQLRNDPVDGDLDIALIIQDKRLWDAKPDWHPDPG
                     RKGKIIEIFGCPAQKESLQLAHIANAKAAIILADPLHGMLSDARSTLVGIAIERENPQ
                     VHTVMELLLSINRTHLRATAVDEVICLGELSEKLLSQSAVSPGVSRLFAHLLTSSVGT
                     AQLFVRPMTGALAGSTYRELAKRAIRNDAPYVVCGYIRPRPRPQSERQENVVVFNPRV
                     DTTPGKDSPLDERDRLILLASTPPDLSLLHG"
BASE COUNT          511 a          745 c          646 g          561 t
ORIGIN      
        1 ccaccgtcgt gatcgaacat acaaccgatg cggttcggca tcccttgctg catcaaggtt
       61 tgctcatcgc cgaagccgcc ttcaccctcc tatttaccat cgaatacgtg ttgcgctggt
      121 atgcgtcacc aaaccggtgg cgctatccgt tcaccaccta cgcgctcatc gatctcatcg
      181 ccatcttgcc ctcatttttg atgttctccc acgtgatggg catcggttcc gagttcttga
      241 tgctgcgaac cgtccggctc ttgcgcacac tgcgtttttt acgcttgctc cggctgctgc
      301 gcatcctccg acacggctac acgctctatc ggctcgctgt cgtcacgcgc atttacgctt
      361 ccgcgctcgt ttatcaatat cgcctcggtc gcctcgcccg tctgggcctc atttccttgg
      421 cctcatggat tgcaggggcc aacctggtgc atctcatcga aagactcagc accacggcga
      481 actcacaaag cccttatgcc tccgactatt gggaatccta ttggggcgtg cttgtcttca
      541 tcatctccgg tatggacgcg ccggagccca tctccctcgg cggccgaatc gtcgtcacct
      601 tgctgctgct cgtcggcatc tgtatcgtcg ccgtctttac aggcgaggtc gtctccgtac
      661 tcgtacgctc cgaagaacgc agcggacgca tggctctaaa acctcccggg ctcaagctcg
      721 ccgagcatat cgtcatcctc ggtcgcaacg atcacctcga aaaaatcatt cagcaagtac
      781 accacgccct cgccggacga cactatatcc tcgtggtttg ccaggaagcc gaacagcttc
      841 cgtcgcccgg accacgagtt catggtcgtg tttttgcgct cgaaggaaac cctgcccgcg
      901 atgatgtcct caatgccgcc aacctcgaag atgctcgtcg tgtcatcgtc ctatccgcgg
      961 cagacacgca agcaagtcct ctcgagcggg acaatatcgc gctgatgcat gcgatcgcgg
     1021 cggtggcacg caacaagaga atccctctgg tgctcgaatt gcaggaaccc gaaagcctgc
     1081 gttatgcgtc atcgattcaa tcggccgatt gcttggtgag ccgctctttc ggtgaaatgc
     1141 tgatgagcca atcggttcac aacaagggcg tcgccgaaat ctacgatgaa ctcatgacct
     1201 tttctcccaa taccaacgag tttcaccgcc tgcgggttcc tccctccctc gttggctcca
     1261 cgtttcggca agcccaggaa tacttcctcg atctcgactc ggaagccatt ttgttgctgg
     1321 gaatcgatgg cccggaacag caggcctacc aggattttcg tctttgcgtc ggaaacgacg
     1381 cggacaatcc gttgcgggat cacgtgctca cggcctccga ctacttgatt gtttgcgctg
     1441 tcgaacgacc tggttttgag ccccatcatc cggagaaggc atggcagacc cacgaacttc
     1501 cccgccgctg agcggccacg tcgtgatttg caacgtgaac gagaaggttc cggccattgt
     1561 ggagcaactt cgcaacgatc ccgtggacgg agatctcgat attgccctca tcatacaaga
     1621 caaaaggctg tgggatgcaa agccggattg gcacccagac ccggggagaa aagggaaaat
     1681 catagaaatt tttggctgtc ccgcgcaaaa agaatcgttg cagctcgctc atatcgccaa
     1741 cgcaaaagcc gcgatcatct tggcggatcc ccttcatggc atgctttccg acgcccgaag
     1801 cacgttggtg ggcattgcga ttgagcggga aaaccctcag gtccacacgg tcatggaatt
     1861 attgctttcc atcaaccgaa cgcacctgcg cgccacggcg gtcgatgagg tgatttgttt
     1921 gggggaattg tcggagaaat tgctctccca aagcgcggtg agccccggtg tgagtcggct
     1981 attcgcgcac ttgctgacct ctagcgttgg cacggcgcag cttttcgttc gacccatgac
     2041 cggcgcgctc gcgggctcta cctatcgtga attggccaaa cgcgccattc gcaacgatgc
     2101 gccttatgtc gtctgtgggt atatccggcc ccgtccgagg ccgcaaagcg agcggcagga
     2161 aaacgttgtg gttttcaatc cacgggtaga tacgacgcct ggcaaggata gcccgttgga
     2221 tgaaagggat cgtttgattt tgttggcctc gaccccgcct gatctatcgc tgctgcacgg
     2281 ttaatccgcc ttccggcaat caattaagtt caatcatttc aattagttac cgttatattg
     2341 ccgcatattt gcgacgggga gggcaagaaa agggcgcaag gggcctcctg ccgccaggag
     2401 aagcgaccag gaggtgagaa gagaaaaaag ggaaaatcag gaagggtcta gctcaatcac
     2461 tca
//
LOCUS       DAOSOR010000729         2462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_42597_length_2462_cov_3.404238, whole genome shotgun sequence.
ACCESSION   DAOSOR010000729 DAOSOR010000000
VERSION     DAOSOR010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42597_length_2462_cov_3.404238"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1940
                     /locus_tag="PK710_24205"
     CDS             <1..1940
                     /locus_tag="PK710_24205"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT12906.1"
                     /translation="LLSAWSAFRLPTVIAAILIPCVTEAAAGLWIWTARSAPPSDLVD
                     WIALCLAITAAATISAIGPALGTALLNRKRRKWIAALVLAVAGSVLAWIDLTAFVSLY
                     PFLHALLEAATTVLWLLSFYLLLETYVARSKYFLFFAAACAIVAVGFIAFHGPSWSAR
                     HLRHTATHHVYVGRLLARTHHLPTLSTHTIRRQPVAEAAQRSMSRQDERPADACPSLW
                     WPKEAPATSVSLRAEMPEAPNIVVVFVDTLRADIAHDPRIMPNLSRFSRASLQFTRAY
                     STGSDTRSCLPAMVRGSYDLDRDDEYDPIYLARANGIPTGLAIASSPREFLAKHVPQF
                     RFDEVLEVPDYDDDKKVWGYGAHRFTAERLVDESIAWIESKPNKRFLLWQFHFDLHAW
                     RELDPKELANQARSLGVPKLRGEWNRYAAAAATIDAAFGRFLRELERLDVRKNTVIIF
                     LADHGEGMGRQGFWLHSVFLWESLVRVPLVVHLPGIEGRKIDSYVSTVDVGPMLARLI
                     DPNASLFPYHGEDLLTQIGPEPASRRFPMLMRAVLKEQVVRVGVIDPTINRKLILPLE
                     SGTPELYDLSVTDPDEMDKALEETATVEKLLPIVNDGPMNPRARRKHDRCLRSLSEPD
                     QDDSLATEGRRMKRAKTGGPT"
     gene            1999..>2462
                     /locus_tag="PK710_24210"
     CDS             1999..>2462
                     /locus_tag="PK710_24210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12907.1"
                     /translation="MLQSKESEDPPGNSSLAADPSLGPTLALTHSITESMAPLNDGAT
                     PISTNEFNDLALDDSPLLPSHDESIQLDAAAGLTNPIAPDVESRAAPAADPTVPIEKR
                     VEAFEDLMDSEIGDTSLSRARNIERTIGLRQLYLKFEGGNPTGTQKDRIAFA"
BASE COUNT          525 a          783 c          611 g          543 t
ORIGIN      
        1 tccttctatc tgcctggtca gcattccgat tgccaaccgt cattgccgca attctgatcc
       61 cctgcgtgac cgaagccgcc gccggactgt ggatatggac cgcaagaagc gcccctcctt
      121 ccgacctcgt cgattggatt gccttatgcc ttgccatcac cgccgccgct acgatttccg
      181 cgatcggccc agccctgggc accgcgcttc tcaaccgaaa acgacgaaaa tggatcgccg
      241 cgttggtgtt ggccgtcgcc ggatctgtct tggcctggat cgacctgacc gcgttcgtgt
      301 ctctctatcc attcctgcac gcgctgttgg aagctgccac aaccgtgctc tggcttcttt
      361 ctttctactt gctcctcgaa acgtacgtgg cgcgttcaaa atacttcctc ttctttgctg
      421 ccgcctgtgc gatcgtcgcc gtaggcttca tcgcgtttca tggcccctct tggtccgctc
      481 gacatcttcg tcacaccgcc acccaccacg tgtatgttgg acgccttctc gctcgtactc
      541 atcatctgcc cacactgtcg acgcacacga ttcgtcgaca acccgtggcc gaagccgcgc
      601 agcgctcgat gtcgcgacag gatgaacggc ccgcggacgc ttgtccttcg ttgtggtggc
      661 ccaaagaagc acctgccacc tccgtgagct tgcgtgcgga aatgccggaa gctcctaata
      721 ttgttgttgt ttttgtagat acgctacgag ccgatatcgc ccatgacccg cggatcatgc
      781 ctaatctttc gcgtttctcc cgcgcttcct tgcagttcac acgtgcctat tcgaccggct
      841 cggatactcg gagctgcctt cctgccatgg tccgaggaag ctatgacttg gaccgagacg
      901 atgaatacga cccgatttat cttgcgcgcg cgaatgggat tcccacgggc ttggccatag
      961 cttcatcccc ccgtgaattc ttggccaagc acgttccgca gttccgcttt gacgaagttc
     1021 tcgaagtgcc tgactacgat gacgacaaaa aagtatgggg ttatggcgca caccgattca
     1081 ccgcagaacg gttggtcgat gaatccatcg cctggattga atcgaaaccg aacaaaagat
     1141 ttttgctctg gcaatttcat ttcgacttac atgcatggcg tgagttggat cccaaagaac
     1201 tcgccaacca ggcgcgttcg ttgggagttc ccaaactccg aggcgaatgg aaccggtatg
     1261 ccgcggccgc cgccaccatc gatgccgctt tcggacgctt ccttcgagaa ttggaacgtc
     1321 tggatgtacg aaaaaatacg gtgatcatct tcctcgcgga ccacggcgaa ggtatgggac
     1381 gacaaggctt ttggcttcat tccgtgttcc tatgggaatc gctcgtgcgc gttcccctcg
     1441 tcgtgcatct tccgggaatc gaaggacgaa aaatcgactc ctacgtgtct accgtcgacg
     1501 tcggtcccat gcttgcccgg ttgattgacc ccaatgcctc cctatttccc tatcatggtg
     1561 aagacctgct cacccagatt ggccctgaac ccgcctctcg gcgattcccc atgctgatgc
     1621 gcgccgtgct caaggagcaa gtcgtgcgtg tgggcgtgat cgaccccacc ataaacagaa
     1681 aactcatcct gcccctcgaa agtggaaccc cggaactcta tgacctatcc gtgacggacc
     1741 ccgacgaaat ggacaaagcg ttggaagaaa cggccaccgt cgaaaaactg ctgccgatcg
     1801 tcaacgatgg ccccatgaac cctcgtgctc gtcgaaaaca cgaccgctgc ttacgttccc
     1861 tttccgagcc cgatcaggat gattcgcttg ccacggaagg tcggcgaatg aaacgtgcga
     1921 aaacaggtgg acccacatga ccctttttca gagggaaagg accacctcga cgacataacc
     1981 atgaaatgag gtacgctgat gctgcaatcc aaagagagtg aggacccccc aggaaattca
     2041 tcgcttgcgg cggacccttc gctcggcccc acgcttgctc tcacgcattc catcaccgag
     2101 tccatggccc ctctgaacga tggcgcgaca ccaatatcca ccaatgaatt caatgactta
     2161 gccttggacg acagcccgct gctgccgagc catgacgaat caatacaact cgatgcggcc
     2221 gcgggcctta ccaatcccat tgcaccggat gtcgaatctc gcgccgctcc tgccgccgac
     2281 ccgaccgtac ccatcgaaaa acgagtcgaa gcattcgaag acctgatgga ctcggagatc
     2341 ggggatacct ccctgtcacg cgccagaaat atcgaacgaa ccatcggatt gcgccaactg
     2401 tatctcaagt tcgaaggggg taaccctaca ggtacacaaa aagaccgcat cgcattcgca
     2461 ca
//
LOCUS       DAOSOR010000730         2454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_42808_length_2454_cov_3.466028, whole genome shotgun sequence.
ACCESSION   DAOSOR010000730 DAOSOR010000000
VERSION     DAOSOR010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42808_length_2454_cov_3.466028"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            323..1231
                     /locus_tag="PK710_24215"
     CDS             323..1231
                     /locus_tag="PK710_24215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12908.1"
                     /translation="MRCLIPAVLLISLLALGCGSTDASLESSGGGADASDAVMDAADA
                     GDTLEAGQTTETNEAADDAIIDVASEVSIEGGSGGDADADATSDDAEAGDDALDCNAL
                     SAEIAAQTQVIGSTSVVVRLDYTSLKVLGQVAVKGGYAGGMKEQVARDVAQKDTGYGG
                     GTLLSGPDPEDEWVFFTSAGDFGGSAAVSARTGLTVFGGSTVWAGKGDINYPKDWNLI
                     DLGSGCPPRERRPARGFDLGQGQPLAQAKVDAAVDVVWQTALPAALMQWGYVFDVLVL
                     LYPRSVGAFDPTTAEYVVIVNAGWLE"
     gene            complement(1361..>2454)
                     /locus_tag="PK710_24220"
     CDS             complement(1361..>2454)
                     /locus_tag="PK710_24220"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOT12909.1"
                     /translation="ATQAPCQGVIGPMPVEPTPLAQATHFAAPNGTSKNCTEAAPCDV
                     WTAIANAGPGSVVFLRGGVYPVSKSIAFHQQGTQTGPITIESYPGELAIFDGSSLKPG
                     TQVYIRVTGAFYHLRRFEVRHMPMQGIWIGGTDNTLDGIVAHHNKLSGIQIFSPYEEY
                     PYGKYGSRNTLRNCVAHDNSGVGLLDPEFADGGNSDGISISSGADNEVDHCLVYLNSD
                     DGIDTWRSTGSSITCSVARQSGLGSGDGNGFKAGGKPPSDGTVVRRCISYRNKQTGFD
                     YNSGKNVVFEHVTAVEDHRGFWTGDDTQLMNSVVVGDSVQGYGSGQITGNCWQTGGSP
                     EFISMDPTSPDFMRPLPGSPCSQLGAAGQ"
BASE COUNT          493 a          652 c          800 g          509 t
ORIGIN      
        1 ttgaaccctt ggacccttgt catctgggat ctggggtcgt cgtaagggag cgaagggagc
       61 ttgccccgcg aagcgagcgc gcttgcggcg ctcgcagcgg ggaatgcgac cgtagcgtac
      121 tggagacggc ccggggggcc gaggggggga gaatcccccc ctcgttttga aaaatgcgtt
      181 gaaaatgtgg cgcttacagt gaattggagc ggttttgggc tgctcggttt ccatcaaaac
      241 aagaaggccg ctggttggcg tgacggtgta ttgatcacgc agcgacttga tcccgcgccg
      301 acgatcgatc atcttgaatc ccatgcgttg cctgattcct gcggtattgc ttatctcgct
      361 cctcgccctc ggctgtggga gcacggatgc ctcgttggaa agctccggag gcggagcgga
      421 tgccagcgat gcggtcatgg acgcggccga tgcgggcgat acgctcgaag caggccaaac
      481 gacggaaacg aatgaggccg cggacgatgc catcatcgac gtggcgagcg aggtgtcgat
      541 cgaaggggga tccggtggcg atgcggacgc cgatgccacc agtgacgatg ccgaagccgg
      601 tgacgatgcg ctcgattgca acgcgctttc tgcggaaatc gccgcgcaaa cgcaagtgat
      661 tggttccacc agtgtcgtcg ttcgactcga ttatacgtcg ctgaaggtgt tgggacaggt
      721 ggctgtcaaa ggggggtatg cggggggcat gaaagaacaa gtggcgcgcg acgtcgcaca
      781 aaaagacacg ggctatggcg gcggaacgtt gttgtccggc cccgatcccg aggatgagtg
      841 ggtgtttttc acctccgcgg gtgattttgg aggctccgcc gccgtgagcg cgcgtacggg
      901 actcacggtg ttcggcggtt ccaccgtctg ggcaggcaaa ggcgatatca actatccgaa
      961 agactggaac ctgatcgatc tcggcagcgg ctgcccgccc cgggaacgac gacccgcgcg
     1021 cggtttcgat ctgggtcaag gccagccgtt ggcgcaagca aaggtcgacg ccgcggtgga
     1081 tgtcgtctgg caaaccgcgt tgcctgccgc actcatgcaa tggggctatg tttttgatgt
     1141 gctcgtgctg ttgtacccac gctcggtggg ggccttcgac cccaccacgg ccgagtatgt
     1201 cgtcatcgtc aacgctggtt ggctcgaatg accggacgat cgtcgtgggg caagaacaac
     1261 gagcgtatag ataaaaatgt ataaatatca atgggttatg gaaagttggg cggtggatgc
     1321 aggccttccg atgagctgat gatgcgggag ggaacgccga tcattgtcca gccgcgccga
     1381 gttgcgaaca gggcgagcca ggcaaaggac gcatgaagtc aggagaagtc ggatccatgc
     1441 tgataaattc cgggctgccg cccgtttgcc agcaattgcc cgtgatctgt ccactgccat
     1501 agccctgaac gctatcgccg acgaccacgc tattcatgag ttgcgtatca tctcctgtcc
     1561 agaagccccg atgatcctcg accgcggtca cgtgctcaaa gaccacgttt tttcctgagt
     1621 tgtagtcgaa accggtctgt ttgttgcgat aggaaatgca gcggcgcacc acggtgccgt
     1681 cggagggcgg tttcccgcca gctttgaatc cattgccatc cccgcttccc aaaccacttt
     1741 gacgagctac cgagcaggta atcgaactgc ccgtggatcg ccaggtgtcg atgccatcat
     1801 cggaattgag gtataccaaa caatggtcga cttcgttatc ggccccggac gaaatggaga
     1861 taccgtcgga gttacctcca tccgcgaatt ccgggtcgag caggccgacc ccggaattgt
     1921 cgtgcgcgac acaatttcga agggtgttgc gggagccgta cttgccgtag ggatattctt
     1981 cgtaagggct aaagatttga ataccactta gtttgttatg atgggctacg attccatcga
     2041 gggtattgtc cgttccgcca atccagatac cttgcatggg catatgacga acttcaaacc
     2101 ggcgaaggtg gtagaaggcg cccgtcacgc ggatgtacac ctgcgtgccc ggcttgaggc
     2161 tggagccgtc gaatatggcc aattcacccg gatagctctc gatggtgatc ggcccggttt
     2221 gggttccttg ttggtggaag gcgatggatt tcgagaccgg gtacacacca ccgcgtaaaa
     2281 agacgacaga tccggggccg gcgttggcaa tcgcggtcca cacgtcgcaa ggcgcggcct
     2341 ccgtgcaatt cttgcttgtg ccgttgggag ccgcgaagtg ggtagcttgt gcgagggggg
     2401 ttggttcgac gggcatgggg ccgatgacgc cttgacaagg agcttgtgtt gcgc
//
LOCUS       DAOSOR010000731         2453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_42824_length_2453_cov_5.015430, whole genome shotgun sequence.
ACCESSION   DAOSOR010000731 DAOSOR010000000
VERSION     DAOSOR010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42824_length_2453_cov_5.015430"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PK710_24225"
     CDS             <1..250
                     /locus_tag="PK710_24225"
                     /inference="COORDINATES: protein motif:HMM:NF039580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12910.1"
                     /translation="DLIRRALAIGDKMFLSGEIERREALSSRIIENAFLSFVDQGYIE
                     QTEANKLALVSSFADPAAVRTIENRIMATLTRRSEDRL"
     gene            247..1080
                     /locus_tag="PK710_24230"
     CDS             247..1080
                     /locus_tag="PK710_24230"
                     /inference="COORDINATES: protein motif:HMM:NF015376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HOT12911.1"
                     /translation="MTYRFSLFLGIAAVVSGAMACVGSPSPLVPEARGSVGLPYRGVL
                     TEPVALPQQGTGYVRIKPAGKHYGTRALVQTIEYAAAEVQRQRPGPPLQIGDLSGPYG
                     GRIPNHSSHRTGRDVDFIFYATDLGGAMVPAQGWTHYGADGLGIAHEGKHGRIYLRMD
                     LERNWLLVKALLQAPTANVMWLFVSNPLRALLTEYAFARGEDPLIVWQAENVLHQPRN
                     SLPHDDHFHLRIMCPEGSEGLACEQGGPVWPWLTPVTLDWPESSSEIATFIEAATIPS
                     P"
     gene            1077..2009
                     /locus_tag="PK710_24235"
     CDS             1077..2009
                     /locus_tag="PK710_24235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOT12912.1"
                     /translation="MTSSHLLAHADAEEFLRALCDIEPAGIDLCYVDPPFNVGSAFCA
                     RKQKGEYRGKARWSSGELAYDDRWGGIEGFLRFLEPRLGAIWKVIARGGMVWIHLDHR
                     AVHETKSLCDSLFGKDAWLGEVIWVPGNGARGRGFSVTHQTLLIFGKRDRHRPFVWND
                     DHPTLREAFARTSLEMHFRAVDENGRRYRERVIAGKRYRYYADEGRKLGSVWTDIPAM
                     VANTPIHRQGTGYPTQKPERLLERIIRWVTIPGALVCDPMCGSGTTLVVAARLGRRFV
                     GSDKSPIAIEMTANRLRSEGIEFESWRNHQATTL"
     gene            complement(2103..>2453)
                     /locus_tag="PK710_24240"
     CDS             complement(2103..>2453)
                     /locus_tag="PK710_24240"
                     /inference="COORDINATES: protein motif:HMM:NF016356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HOT12913.1"
                     /translation="NVLSIIAHCGFLPVCPEQLGGLSTPRARHEIIGERVVSEYGDDA
                     TDAFERGAQQAFLLVTLSAARLAILKARSPSCGKGQVYDGTFTGRLCAGHGLFAKRLI
                     EYGIRVLTEDEITQ"
BASE COUNT          530 a          670 c          713 g          540 t
ORIGIN      
        1 ggacctaata cgtcgcgcgc tcgccatcgg agacaagatg ttcttgtccg gtgaaatcga
       61 acgtcgtgaa gcgttgagca gccgaatcat cgaaaacgcg ttcctatctt tcgtcgatca
      121 aggctatatc gagcaaacgg aagccaacaa gctcgctctg gtttcgtctt ttgccgatcc
      181 cgccgcggta cgcaccatcg agaatcgaat catggcgacg ttgacacgac gttccgaaga
      241 ccgactatga cgtatcgttt ttctcttttc cttgggattg ctgccgtggt ttcgggtgcc
      301 atggcgtgtg tcggttcgcc ttcgccgctc gtacccgaag cccgaggctc tgttggtctt
      361 ccctatcgcg gtgtgttgac cgaacctgtc gcgcttcccc agcaaggaac cggctacgta
      421 cgtatcaaac ccgcgggcaa gcattatggt acgcgagcac tggtgcagac gatcgaatac
      481 gcggccgccg aggtgcaacg tcaacgccct ggaccaccgc tgcagattgg ggatttgtcg
      541 ggaccgtacg gcggacgtat ccccaaccac agctcccatc gcacgggacg ggatgtggat
      601 ttcatttttt atgccaccga tcttggtggt gccatggttc ctgcccaagg ctggacccat
      661 tacggtgccg atggcctggg catcgcccat gaaggtaaac atggccgcat ctatttgcgc
      721 atggatttgg agcgcaactg gctcttggtg aaagcgctgc tgcaagctcc cacagccaat
      781 gtcatgtggc ttttcgtcag caacccactc agggctttgc ttacggaata cgcatttgct
      841 cgcggagagg accctttgat cgtttggcaa gccgagaatg ttttgcatca gccacgcaat
      901 tccttacccc atgacgatca cttccatctt cgaatcatgt gtccggaggg aagtgagggg
      961 ctggcgtgcg agcagggagg gccggtctgg ccttggttga cacccgtcac gctcgattgg
     1021 cccgaaagct cctccgagat cgcaaccttc atcgaggccg caaccatccc ttcgccatga
     1081 cgtcgtcgca tctcctggct cacgcggatg cggaagaatt tttgcgcgcg ttatgcgaca
     1141 tcgaacccgc gggaatcgat ctttgctatg tggatccgcc cttcaacgtc ggatcggcat
     1201 tttgcgcacg aaagcaaaaa ggcgaatacc gaggcaaagc acggtggtca tctggcgagc
     1261 ttgcctacga tgatcgatgg ggaggtatcg aaggatttct tcgatttctc gagccgaggc
     1321 tcggagcgat ctggaaagta atcgcgcgcg gtggcatggt gtggattcat ctcgaccacc
     1381 gcgcggttca tgaaaccaaa tcgttgtgcg attcgctatt tgggaaagac gcctggttgg
     1441 gagaagtcat ctgggtaccg ggtaacgggg ctcgagggcg aggtttcagt gtgactcatc
     1501 aaaccctgct cattttcgga aaacgcgatc gtcatcggcc tttcgtttgg aatgacgacc
     1561 accccacgct gcgcgaagcc ttcgcacgaa cgagtctcga gatgcacttt cgcgcggtgg
     1621 atgaaaacgg acgtcggtac cgcgaacgag tcattgctgg taagcgatat cgatactacg
     1681 ccgatgaagg gcgcaaactg ggcagcgtat ggacagatat tcctgccatg gtcgccaaca
     1741 cgcccattca cagacagggc actggatatc caacccaaaa gcccgagcgg cttctcgaac
     1801 gcatcatccg ctgggtgacg atccccggtg cgctggtatg cgatcccatg tgcggttcag
     1861 gcacgactct ggttgtagcc gcgcggctcg ggcgacgatt cgttgggtcg gacaagagtc
     1921 ccatcgcgat tgaaatgacg gcgaatcgtc ttcggagcga aggcatcgag ttcgagtctt
     1981 ggcgaaatca tcaagccacg acgctgtagg gggtgggacg ttcgtgtctg cggaaagagg
     2041 ctgaaaaaaa acgtaaacac cgaggctccc ttcgaccgcc gatcgattcc aacaacatga
     2101 gtttattgag ttatttcgtc ttccgtcaga acccgaattc catattcgat cagccgtttc
     2161 gcaaataagc catggccggc gcataagcgt ccggtaaagg ttccgtcata cacttgacct
     2221 ttgccacacg aaggagatcg cgctttgaga atggcgagac gtgcggctga caacgtgacg
     2281 agtagaaaag cttgttgggc gccacgttcg aaagcatccg tggcatcatc tccgtattcc
     2341 gagaccacgc gctcaccgat gatttcgtga cgagcacgag gggtggatag gccgccgagt
     2401 tgttcggggc agacggggag aaatccgcag tgtgcgatga tggaaaggac att
//
LOCUS       DAOSOR010000732         2450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_42900_length_2450_cov_3.292276, whole genome shotgun sequence.
ACCESSION   DAOSOR010000732 DAOSOR010000000
VERSION     DAOSOR010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42900_length_2450_cov_3.292276"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="PK710_24245"
     CDS             complement(<1..1108)
                     /locus_tag="PK710_24245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12914.1"
                     /translation="MENRRSRGERYSTREVVAILVPLCTDLIARHERGEQLLLTPSFI
                     RFQVGSPTALIPGQPSSGLPRDRACLPPESRTGKGGDARSTVFSIGAIAYEMLTGDAV
                     GPGMRRPSEIVPSLPAIVDTILSKALIGDPSHRPPDLGALAQALHNLAPTASQAPPPI
                     DEAALDDEDFEVDISLSMVPPPPKAAVAPLPPPAPIPHDLMGTDDGGLVAVARAPRAP
                     DPIDPTSALAQLRDRLEADPRPRYVVIKDGMDHGPFSAVELLQQIASHTFEAQHILVD
                     SFSQDERPISDWDEFAPFAEHAKLNKQIVAEKIELERSVVAESKTTRNKALFISGSLA
                     IVAVIGISAWFIAKPKTRSGEVLVDAHSALNIEFD"
     gene            1347..2243
                     /locus_tag="PK710_24250"
     CDS             1347..2243
                     /locus_tag="PK710_24250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT12915.1"
                     /translation="MSVPTSRVTVVMVVEDDPVNRMLLGEVCRGEGYEVLLADDGEMA
                     LSQFMERDIDVILVDGAMPRMDGFAFSRWVRSLSDVPIIMVSASIEPGVQRKARDAGV
                     TTFISKPFRVYELTRQILAVMRGVSPSEPPGGQRRRLAAVLDRLRTSKPLRADLGRAI
                     QQGGVCLVLRLENEQDVVRRIGRTGRDALLGYAGQGLEGYWGHESVYWTDSHELAVVV
                     TASQFEGLMEMLRGLLEEMQRFDVGDVLFRYGAVRFQPMQGLDPDEVIRVARHAANEA
                     GHRGEQGRIEEIGERVEPAGSE"
     gene            2253..>2450
                     /locus_tag="PK710_24255"
     CDS             2253..>2450
                     /locus_tag="PK710_24255"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT12916.1"
                     /translation="MSEIMDWLGFGPSDLRAPLTGREQEMRDLEQALAEVVDARESRL
                     VTVMGASGAGKSRLVHDFLTRL"
BASE COUNT          480 a          505 c          935 g          530 t
ORIGIN      
        1 catcgaattc gatattgagc gcggagtgag catcgacgag cacttctccg ctgcgagttt
       61 tcggtttggc aatgaaccaa gcgctgattc cgatgaccgc gacgatcgcc agcgagccgc
      121 tgataaaaag agctttgttg cgagtggttt tggattctgc caccacggag cgctcgagtt
      181 cgattttttc cgcgacgatt tgtttgttga gcttggcgtg ctcggcgaat ggggcgaact
      241 catcccaatc ggagatgggg cgttcgtctt gagaaaaaga atcgacgaga atgtgttgtg
      301 cttcgaaggt gtgggaggcg atttgttgaa gcaattcgac cgcggaaaac ggtccgtggt
      361 ccattccgtc tttgatgacg acgtagcggg gacggggatc ggcttcgagt ctatcgcgaa
      421 gctgagccaa tgcggaggtg ggatcgatcg ggtcgggggc tcgaggcgcg cgggctaccg
      481 caaccaggcc gccatcgtcg gttcccatga gatcatgggg aatgggtgcg gggggaggca
      541 ggggtgccac cgctgccttc ggtgggggag gaaccatgga aagcgagatg tcgacttcga
      601 aatcctcgtc atcgagcgct gcttcgtcga tggggggagg agcttggctc gccgtgggag
      661 cgagattgtg aagggcttgc gcaagggctc ccagatcggg gggacggtgg gaaggatcgc
      721 caatcaacgc tttgctgagg atggtgtcga cgatagcagg cagagaagga acgatctcgg
      781 aaggacgtcg cataccgggg cccacggcat cgccggtgag catttcgtag gcgatggcgc
      841 cgatactgaa aacggtggag cgtgcgtcgc cgcctttgcc agtgcgagat tcggggggca
      901 agcaggctcg gtctctaggc agtccggacg atggttgtcc tggaatcaaa gcggttgggc
      961 tacctacttg aaagcgaatg aaagaggggg tgagcaagag ttgttcaccc ctttcgtggc
     1021 gtgcgatgag gtccgtgcag agcgggacga gtatcgcgac cacctcgcgg gtagagtaac
     1081 gttcgcctcg ggatcgtcgg ttttccatat ccgcgcggag cgttccgggc gggatgacga
     1141 cgttggcggg gaggggtgtt aaaccagggc cagcttgagt cattgaaagt cgaagatacg
     1201 acaggggcgg tgggctctca atacagacga gtctgaagtg agcgaaggag gtgtaagtcg
     1261 tgaagaaata acgcattgcg ttgtcaatga tggggtcgtg agaggtcggc ctttgggttc
     1321 cagcgacctt gggctaggat tcgagcgtga gcgttccgac gtcgcgcgtt acggtagtca
     1381 tggtggtcga agacgaccct gttaatcgga tgctgcttgg ggaggtgtgt cgcggtgagg
     1441 ggtatgaggt gttgctggcg gacgatgggg agatggcgct cagtcagttt atggaacggg
     1501 atattgatgt gattttggtg gatggtgcga tgccgcgcat ggacggattt gcgttttctc
     1561 gctgggttcg gtcgttgagc gacgtgccga tcatcatggt ttcggcgagt atcgagccgg
     1621 gggtgcagcg caaggcgcgg gatgctgggg tgacgacgtt catatcgaag ccgtttcggg
     1681 tttacgagct gacgagacag attctggcgg tgatgagggg ggtatcgccg agtgaaccgc
     1741 cgggggggca acgtcggcgt ttggcggccg tattggatag gctgaggacg tcgaagccgt
     1801 tgcgtgcgga cttggggcga gcgattcagc aggggggagt ctgtttggtt cttcggttgg
     1861 aaaacgagca agatgtggtt cggaggatag ggcgtacggg acgggatgct ttgcttgggt
     1921 atgcggggca ggggttggag gggtattggg ggcatgagtc cgtgtattgg actgacagcc
     1981 atgagttggc cgtggtggtt acggcatctc agttcgaagg gttgatggaa atgttgcgag
     2041 gtctcctgga ggagatgcag cgttttgatg tgggtgatgt gttatttcgc tatggggccg
     2101 tgaggtttca acccatgcag gggcttgacc ccgatgaggt gataagggta gcacgccacg
     2161 cggcgaacga agctggacat cggggagagc aagggcgtat cgaagagatt ggcgagcggg
     2221 tggagccagc aggaagcgag tgaacggtca atatgtctga gatcatggac tggctgggct
     2281 ttggtccgag tgacttgcgt gcgccgctaa cgggacgcga gcaagaaatg cgtgaccttg
     2341 agcaagcgtt ggcggaagtg gtggacgcac gagagtcgag gctcgtgacg gtgatggggg
     2401 cgtccggagc agggaaaagt aggttagtgc atgatttttt gacgaggctc
//
LOCUS       DAOSOR010000733         2441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_43104_length_2441_cov_4.040654, whole genome shotgun sequence.
ACCESSION   DAOSOR010000733 DAOSOR010000000
VERSION     DAOSOR010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43104_length_2441_cov_4.040654"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PK710_24260"
     CDS             complement(<1..490)
                     /locus_tag="PK710_24260"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HOT12917.1"
                     /translation="MSRTDFLSKVARRVAEPASNAPPGVKNSILTVAAGSYGLRPGGD
                     DVTMPTGFDPRAAALFESIVEASYLVATADGVFDDAEKNAFRTIVLEACRGAVASDSL
                     EALLADLADQLEEDGLDHRVQMVSKTINRTDQQREVLRIATFLAFASGGVSDAELDVI
                     EKM"
     gene            complement(557..1846)
                     /locus_tag="PK710_24265"
     CDS             complement(557..1846)
                     /locus_tag="PK710_24265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOT12918.1"
                     /translation="MNSWKGLAIATSLAALVVGVVVACGGDDEDNGGSGGSSASSGSG
                     GTAGKGGSGTGGSAADSGSGGSYIITEGGVGDTSYPDGFDPDAACASQDFKATLTAAN
                     LLFVIDRTGSMNCNPPPTTDSEKCEVQPVKENPNEPSKREITRDALTAAWQGLVNSDP
                     LPSVGVMLFNNDDFCGFPASPDVVVGPMNDPQLNELVLKLGEVKPKGATPIIGATMSA
                     YNYLWKNKDSFTGNKFVVLLTDGAETCDKDVASKAYLIGKANEAASVGIRTFVLGAPG
                     SERERAFLSQIAWAGGTAKSATCDHSGSDPEVGDCHMDMTLQNMNFAQELQKNLEAIS
                     GEALSCEFEVPQPGPNDPAIDYNKVNVVYTHGDGTKETIPQDTTVECSDPTNAGWQYY
                     DNKSKIVLCGSVCDKVKSDPKASISIELGCVTVVVPK"
     gene            complement(2018..>2441)
                     /locus_tag="PK710_24270"
     CDS             complement(2018..>2441)
                     /locus_tag="PK710_24270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOT12919.1"
                     /translation="GGPDVFAHNVEVVRRMTRRVRDARCDYDRSLAVLAYAKKRVPER
                     FIKSSLMVGAGETDEEVEVCLSDLRQVGVDIVTVGQYLRPSPKHMPVDRYVEPERFEA
                     YRRMGERMGFRFVASGPLVRSSYRAAEAFVRAERGVVD"
BASE COUNT          553 a          868 c          507 g          513 t
ORIGIN      
        1 tcattttctc gatgacgtcg agttccgcat cgctcacgcc ccccgaggca aaagccaaaa
       61 acgtggcaat gcgcaacacc tcccgctgct gatccgtcct attgatggtt ttggacacca
      121 tttgcacacg atggtccaga ccatcctctt cgagttgatc cgccaaatcc gcaagcaatg
      181 cttcaagcga gtccgaagcc accgcgcctc gacacgcctc caatactatc gtcctaaatg
      241 cgttcttctc cgcatcatcg aacacaccgt ccgccgtggc cacgagatac gacgcctcca
      301 cgatgctctc gaaaagtgcg gcggctcttg gatcaaaacc cgtcggcatg gtgacgtcat
      361 ccccacccgg acgtaggcca taagaccccg cggccacggt gagaatcgaa ttcttgacac
      421 caggaggagc attgcttgcc ggctcagcca ctcgccgagc taccttggac aaaaaatccg
      481 tgcgggacat gaggaaatcg tctgcctctc gagttgaatt gtcttggaat aacgaccgtt
      541 atcgaaagaa gaagcgttat ttcggcacca ccaccgtcac acatccaagc tcgatcgaaa
      601 tcgaagcttt gggatccgac ttgaccttgt cgcagaccga accacataac acgatttttg
      661 atttgttgtc atagtactgc caacccgcat tcgttgggtc ggaacattcc acggtcgtgt
      721 cttggggaat cgtctctttc gtaccatccc cgtgcgtata taccacgttc accttattat
      781 aatcgatcgc cggatcgttc ggccccggct gcggtacctc aaactcgcac gacagcgcct
      841 cgccactgat cgcttccagg ttcttctgca gttcctgcgc gaagttcata ttctgcaacg
      901 tcatgtccat gtggcaatcc cccacctccg gatccgaacc ggaatgatca cacgttgctg
      961 actttgccgt tcccccagcc caagcgatct gtgacaaaaa cgctcgctct cgctcgcttc
     1021 ccggcgctcc aagcacgaac gtccgaatcc ccaccgatgc cgcctcattc gcctttccaa
     1081 tgagatacgc cttcgaagcc acatccttgt cacacgtctc cgccccatcc gtcaacaaca
     1141 ccacaaactt gttgcccgta aacgagtcct tgtttttcca caaataattg tacgcactca
     1201 tcgtcgcccc aatgataggc gtagccccct tgggctttac ctccccaagc ttcaacacca
     1261 actcgttgag ttgcggatcg ttcatcgggc ccacaacgac atcagggctc gctggaaaac
     1321 cacagaaatc atcgttgttg aaaagcatca cccccaccga aggcagtgga tccgaattca
     1381 caagcccctg ccacgccgcc gtcaacgcat cacgcgtaat ctcccgcttc gagggctcgt
     1441 tcgggttctc cttcaccggc tgcacttcac acttctcact gtccgtcgtc ggcggagggt
     1501 tgcaattcat cgatcccgtg cggtcaatca caaaaagtag attcgccgcc gtcaacgtcg
     1561 ctttgaagtc ctgcgacgca cacgcggcgt ccggatcgaa accgtccgga taagaagtat
     1621 cccctactcc cccctcggtg atgatgtacg atcctcctga accgctgtcc gcagcagaac
     1681 cgccggtccc cgatccgcct ttcccagccg ttccccccga accactgctc gcagaactcc
     1741 ccccggaacc gccgttgtct tcatcatccc caccacaagc taccaccact cccaccacca
     1801 acgcggcgag actcgtcgca atcgcaaggc ctttccagct attcataacc gtattctcct
     1861 tcacagctcc gattctccca cggcacatcg agtcacgatg ggatacggac ggcgggtgtc
     1921 caagcaagtc actcccaccg ttgtagcacg ttctctgccc atcggaaagc atccccaccg
     1981 aaagagccga ggagccatcc accttacccc atatctttca gtcaaccact cctctttccg
     2041 ctcgcacaaa tgcctccgcc gctcgataac tcgaacgaac caaaggacca ctcgccacaa
     2101 atcgaaaccc cattcgctcg cccatccgac gatacgcctc gaaccgttcc ggctccacat
     2161 agcggtccac gggcatatgt ttcggtgatg gtcgcaaata ttgccccacc gtcacaatat
     2221 ctacccccac ctgccgcaaa tccgacagac agacctccac ctcctcgtcc gtctcccccg
     2281 ctcctaccat caaactcgac ttgataaatc gctcaggaac acgctttttc gcatacgcca
     2341 acaccgccag cgaacggtca taatcacaac gcgcatctcg aactcgccgg gtcattcgac
     2401 gaacaacctc gacgttatgc gcaaatacgt ccggcccccc c
//
LOCUS       DAOSOR010000734         2423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_43489_length_2423_cov_4.221706, whole genome shotgun sequence.
ACCESSION   DAOSOR010000734 DAOSOR010000000
VERSION     DAOSOR010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43489_length_2423_cov_4.221706"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..>2423
                     /locus_tag="PK710_24275"
     CDS             76..>2423
                     /locus_tag="PK710_24275"
                     /inference="COORDINATES: protein motif:HMM:NF039580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOT12920.1"
                     /translation="MVRSYDPSAVLQTAFRRFFDHIQIDEAWLSIVRDAAGRGPVVYV
                     MRNLSFLDFMALDHLTERYGLPGVRFANDLGLWVLEPFAETWTQAVLQSRRTPPTERM
                     SDVISSGGSAVLFLKRPPTLLEKAAHSRRRAVSSEGDALVKTLFDLQGRMDRPIMLVP
                     QVFVWSKRPERLEPSLIDFVLGPRDWPGRIRLAAEFLLNYRNVLLRAGEPIALTQFLQ
                     ANAGETQETLVRRGTYALLRKIERERRAVLGPMRKPADRVREEIVRSPKLTHAIRQLA
                     GEGRAERMLLTARAYRMLRELEAAPDPETIVAMSTAIQGILNVLYAGLEIDKEGIERV
                     RQAAQRGTLVFLPSHKSHIDYLLLSHVLQKHSLQLPLIAAGDNLSFFPAGPIFRRGGA
                     FFIRRSFHGDRLYAAVVDAYLRRVLRDGWPIEVFIEGTRSRTGKLLPPKLGLLNMLVD
                     SGLSISNRDILFIPVSIGYERLVEEGSYVRELLGGEKRTEDTTALIRASAIFAERYGR
                     LNIQFGEALSLRQLMQETGLREGDRVTPARRRELTKKIAYQALGRINAVTAATPGAIV
                     ALALLTHGKRGLTHSELLERSRQLLVTLQRQGARCISSLVTPSGALRPASIREAAKMF
                     QKAKLVQVHIAADSLVGKRVKTSAKTDQDIVYTVPDDKRIALDISKNIIIHFFVPFAL
                     IAIAMLAPPGPPCDLASVRERVERLSKLFKHEFMFSRDKGFDEIFDDTLRSMALAHEI
                     RVEDNQLSFGEGHDGATGRAWLVWYGSMLRNFLEAYVVSARSL"
BASE COUNT          492 a          683 c          681 g          567 t
ORIGIN      
        1 gcaaaggttc tgcaaaccaa gcgttgaccg gcggcattcc ccctgccaaa acctcgcttt
       61 atgtctggaa gagacatggt tcgaagttat gacccgagtg ctgttttaca aacagcattc
      121 cgtcgctttt ttgatcacat tcagatcgac gaggcatggc tttccatcgt tcgcgatgcg
      181 gctgggcgcg ggccggtcgt atacgtgatg aggaatttgt cgttcctcga cttcatggcg
      241 ctcgatcact tgaccgaacg ttacgggttg ccgggcgttc gattcgccaa tgacttgggg
      301 ttgtgggtac tcgagccatt tgccgaaacg tggacacaag ccgtccttca atcacgccgt
      361 acgcctccca cggaacgaat gagcgacgta atctcgagtg gtggctccgc ggtgcttttt
      421 ctcaagcgcc ctcccacctt actcgaaaaa gcagctcata gtcgtcgcag ggccgtgtct
      481 tcggaaggtg atgcgttggt gaagacgctc ttcgatttgc aaggacgcat ggaccgaccc
      541 atcatgttgg tgcctcaagt attcgtttgg tccaagcgcc ccgaacgatt ggagccttcg
      601 ctcatcgatt tcgttctcgg cccacgggat tggcctggac gcattcggtt ggcggctgag
      661 tttttgctca actaccgcaa tgttctattg cgtgcgggtg agcccattgc gctgactcag
      721 ttcctgcaag ccaacgcggg agaaacccag gaaacgctcg tccgtcgggg aacctacgcg
      781 ctcctgcgta agatcgagcg ggaacgtcga gcggtcttgg ggccgatgcg taagcccgcg
      841 gatcgtgttc gcgaagagat cgtccgcagt cccaagctca cccatgccat tcggcaactt
      901 gcgggcgaag ggcgtgcgga acgaatgctg ctgacggctc gtgcttatcg catgcttcgc
      961 gaactcgagg ccgctcccga tccagagacg atcgtggcga tgagcactgc gattcaaggg
     1021 attctcaacg tgctctacgc ggggttggag atcgacaagg agggaatcga acgagtcagg
     1081 caagcggcgc agcgcggtac tctcgttttc ttgccgagtc acaaaagtca catcgattat
     1141 cttcttctgt cgcatgtatt gcagaagcac tcgctgcagc ttcccctcat cgctgccggc
     1201 gacaacttat cgttctttcc cgctggcccg atttttcgtc gagggggcgc gtttttcatc
     1261 cgtcgcagtt tccacggaga ccgtctctac gccgcggtgg tcgatgcata ccttcgtcgc
     1321 gttcttcgtg atggttggcc gatcgaagta ttcatcgaag gaacgcgttc acgaacggga
     1381 aagcttcttc ctccgaaatt ggggctgctc aacatgctcg tagactcggg cttgagcatc
     1441 tcgaatcgag atattttatt cattcccgtg agcatcggct acgaacgttt ggtagaggaa
     1501 ggctcttatg tgcgcgagtt gttgggggga gagaagcgaa cggaagacac gacggcgctg
     1561 attcgagcct ctgcgatctt cgcggagcgt tatggtcggc tcaacattca gtttggcgag
     1621 gccttgagct tacggcaatt gatgcaagaa acgggactcc gtgaaggtga tcgcgtcacc
     1681 ccagcacgac gacgcgagct aaccaaaaaa attgcgtatc aagccttggg acgcatcaac
     1741 gcggtcacgg ccgcgacccc aggtgccatc gtggcgctag cgttgctcac ccatggtaag
     1801 cggggactca cgcattcgga actgctggag cgctctcgcc agctacttgt cactcttcag
     1861 cgacagggag cacgttgcat ctcttcgctc gtcacaccgt cgggcgcatt gcgccccgcc
     1921 tccatccgcg aagcggcgaa aatgtttcaa aaagccaagc ttgtccaagt ccacatcgcc
     1981 gccgattctc tcgtgggaaa acgcgtcaaa acgtctgcaa aaaccgatca agacatcgtc
     2041 tacaccgttc ccgacgacaa acgcatcgct ttggatattt ccaaaaacat catcatccac
     2101 tttttcgttc cgtttgcgct catcgccatc gcgatgcttg ctcctcctgg tcctccttgt
     2161 gaccttgctt cggttcgaga acgggtcgaa cgcctttcca agctgttcaa acatgaattc
     2221 atgttctcga gggataaggg gttcgatgag atcttcgacg acacgctgcg gtcgatggcg
     2281 cttgcccacg agatccgcgt ggaagacaat caattatcgt tcggtgaggg gcacgatggc
     2341 gcaacggggc gagcgtggct cgtttggtat ggatccatgc tccgcaactt cttggaagcc
     2401 tacgtggtaa gcgcacgttc tct
//
LOCUS       DAOSOR010000735         2423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_43493_length_2423_cov_3.985642, whole genome shotgun sequence.
ACCESSION   DAOSOR010000735 DAOSOR010000000
VERSION     DAOSOR010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43493_length_2423_cov_3.985642"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..775)
                     /locus_tag="PK710_24280"
     CDS             complement(5..775)
                     /locus_tag="PK710_24280"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT12921.1"
                     /translation="MIDGKRVCVVMPAYNAAETLEKTVSEVPKTIVDDIILVDDQSKD
                     HTVSIARRLGLHVIVHEKNRGYGGNQKTCYREALRRNADIVVMVHPDYQYTPRLIPAL
                     ASCISSGLYDAALGSRILGGRAIAGGMPVYKYVANRALTAVENLLIGEKLSEYHTGYR
                     AFSRQLLLTLPLEENEEGFVFDNQMLVQAVFFGFKIAEVTCPTVYTAESSSIGFSSSV
                     VYGFGVLNAAVACRLALHGVHVPRFLSAQGKKLFGDEP"
     gene            complement(772..>2423)
                     /locus_tag="PK710_24285"
     CDS             complement(772..>2423)
                     /locus_tag="PK710_24285"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT12922.1"
                     /translation="ILSIGVTVWVQGHGGAVRTIEELPLLARVENVVVSYARYLGILV
                     WPKDLCAFYPFPGRWPIMWTVGSTILLIVITLLGVVMAKRKPFVLVGWLWFMGTLVPV
                     IGLVQVGSQSIADRYTYIPSIGVFMALAWGVDEMAGRFRHGRRWQWAVGVAVSVALAA
                     ATYVQAGRWQNNAILYRHTLAVSRSFNVYAHYFVGIDCFERGDDAEARRHFELIVKAA
                     PAFSKGHAALGTVLAHQRDYDRALTHLKRALVLEPDVADVHLNVAQILDDRGDAAGAK
                     AHYLDVLRLDPESYVANYRLGRLYWRAGDHARAAIHFERALIENPHDHAAKRAFIEAC
                     LALGQRDRAVRECLDWMTWARGVDSWMRLAEQFERLNMLHEAATALAKAVAADPANPE
                     SYHRLGIFLAAYGQTNLAIGYLEVAVRLHGDNSSARMALATALLSLGRTQEAILQLRQ
                     ASIRRPEDTTLALHATGLLLRHGKTQEAISFFQEAWKRNPRLSLAAIDWIEQLHDTDQ
                     RKQAILFFDSLRAFVLENNQHELASAMDALARLLTVKGTRP"
BASE COUNT          557 a          696 c          657 g          513 t
ORIGIN      
        1 ggcgtcaagg ttcgtctccg aaaagttttt ttccttgtgc ggacaaaaac cgaggaacgt
       61 gaacaccgtg caaagccagt cggcaagcca ctgccgcgtt caagactccg aagccgtaca
      121 cgacactgga ggaaaagcca atcgacgatg attccgcggt gtagaccgtg gggcaggtca
      181 cttcggcgat tttgaaaccg aagaacacgg cttgcacgag catttggttg tcgaagacga
      241 atccctcttc gttttcttcg agcggcaagg tcagaaggag ttgccgagaa aaggcgcgat
      301 agccggtgtg atactcggat agtttctcac caatcaacag gttttccacc gcggtaagcg
      361 ctcggttcgc cacgtatttg tacacgggca ttcctcccgc gatggccctt ccgccgagga
      421 tgcgtgagcc aagggcagca tcgtacagtc cactggagat acaagaggcc aacgcgggga
      481 tcaaccgagg cgtgtattga taatccggat ggaccatcac gacgatatcc gcatttcgtc
      541 gaagcgcttc cctgtagcag gtcttttgat ttcccccata gccgcggttt ttttcatgca
      601 cgatcacgtg caatccaaga cgtcgcgcaa tggatacggt gtggtctttg gattgatcat
      661 cgacgaggat gatatcgtcg acgatggtct ttggaacctc agatactgtt ttttcaaggg
      721 tttccgccgc attgtaggca ggcatgacga cgcaaactcg tttgccatcg atcatggtct
      781 tgtccctttt acggtgagta gtctggcaag agcgtccatg gcactcgcga gttcatgttg
      841 attgttttcg aggacgaatg cacgcaagga atcgaagaac aggatggctt gttttcgttg
      901 atccgtatcg tgcagttgct cgatccaatc gatggcagca agcgatagtc ttgggtttcg
      961 cttccatgct tcttgaaaaa agctgatggc ctcctgagtt tttccatggc gtaggagcaa
     1021 cccggtggca tgaagggcga gcgttgtgtc ttccgggcgc cgtatcgagg cttgacgaag
     1081 ctgcaggatt gcttcctggg ttcggcccaa ggaaagcaaa gcagtggcaa gggccatacg
     1141 cgcggacgaa ttatctccgt gcaggcgcac cgccacttcg agataaccga tggccagatt
     1201 cgtttgaccg taggcagcca aaaaaattcc caggcggtga tagctttcgg gattggccgg
     1261 atccgcggcc acagcctttg ccagcgcggt cgcggcctcg tgcaacatat tgagccgctc
     1321 gaattgttcc gccaaccgca tccacgaatc cacgccgcgt gcccatgtca tccagtcgag
     1381 acactcgcga accgctcgat cgcgctgtcc aagagccagg catgcctcga tgaacgctcg
     1441 cttggccgcg tgatcatgag gattctctat taaagctctc tcgaagtgaa tcgcggcccg
     1501 ggcgtgatcc cccgcacgcc aatacagccg tcccaatcgg tagttagcca catagctctc
     1561 aggatcgagg cgcaatacgt cgagatagtg tgccttggct ccagccgcat ctccgcggtc
     1621 atcgaggatt tgcgccacat tgagatgcac gtcggccacg tccggttcca gcaccagcgc
     1681 gcgtttcaaa tgcgttagtg cgcgatcata atctcgttgg tgcgccaaca ccgtacccag
     1741 cgccgcgtgt cctttggaaa acgccggagc tgcctttacg atgagttcga aatgtcttcg
     1801 cgcctccgca tcgtcccctc gctcgaaaca atcgatgccc acaaagtaat gagcgtacac
     1861 attgaatgat cggctgacgg ccagcgtgtg acggtacagg atcgcattgt tctgccacct
     1921 ccccgcttga acatacgttg ccgcggccaa cgccacactt accgcaacgc ctaccgccca
     1981 ctgccatcgt cggccatgac gaaaacggcc tgccatctca tccactcccc aagccaacgc
     2041 catgaataca ccgatcgacg ggatataggt gtagcggtcg gcgatcgatt gcgatcccac
     2101 ttgaaccaga ccgatgaccg gaaccagggt acccatgaac cagagccacc cgacaagaac
     2161 gaacggtttt cgcttcgcca tgacaacgcc aagcagcgtg atcacgatca gaaggatcgt
     2221 ggaacctacc gtccacatga tgggccaacg gccagggaac ggataaaaag cgcagagatc
     2281 tttcggccaa acgaggattc caagataacg ggcataggaa acgacaacgt tttcaacgcg
     2341 cgccaacagg ggcaattctt cgatcgtccg aaccgctcca ccatgtcctt gtacccagac
     2401 cgtcaccccg atggagagga tgg
//
LOCUS       DAOSOR010000736         2412 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_43777_length_2412_cov_3.186254, whole genome shotgun sequence.
ACCESSION   DAOSOR010000736 DAOSOR010000000
VERSION     DAOSOR010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43777_length_2412_cov_3.186254"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1701)
                     /locus_tag="PK710_24290"
     CDS             complement(<1..1701)
                     /locus_tag="PK710_24290"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOT12923.1"
                     /translation="MIRIQLMIILMLLCFVGTAQAAPLSTRFSTNIGSGPVHALAVSG
                     NRLYVGGQSGLSVVDISGSGTPTVLGQISLGAEVTAIYVQGNTVYAGTVNQSGPEFRI
                     VNVSNASSPSVAGSLELGVDVAAIAISGTTAYVGVAAPGGDNPNRGLRIVSVANPSSP
                     SLQGQLLNDTRIFGLKIYKNYLLGVSNSIPAEAAILHVWNISSPANPTHVVSTHEGRT
                     IRAYYVETIGDYAFLGQYHGFDDREVGVFDLSALPNVKSPTSVGLSSRSFGGAASGSY
                     LFAGLETRAPTNFEVIDGSHGPTALRLEGYKAGDNGRITSVAISGLTAYLGLKEGSSN
                     LIGVDVSKYLGPDTIAPTVTLHQPVGGAPLSGKIQLRATATDNIRVTKVDFSVGNTHI
                     ATAHTQSLTSPFFALWDTSTVADGNHTVTATAWDNAGNSSKSSITVMVANAAGDKLAP
                     TVAFVHPDNNVTVGNAILLSATAEDNFAIARVQFQVDGNNFGAAITTPPYRIAFDTTE
                     ISNGSHNVTAIATDTSNNSAQATATFTVDNRPAWYSVFTPKRQVYVSPNGNGNGSSAS
                     R"
     gene            complement(2192..>2412)
                     /locus_tag="PK710_24295"
     CDS             complement(2192..>2412)
                     /locus_tag="PK710_24295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12924.1"
                     /translation="EPPLGCRDSTSELLGAAGRFLGRRQSQIETAFLSLYAVTRSAAK
                     HVSEAKSPVVPERPEEPGAQGHRRLIRG"
BASE COUNT          535 a          646 c          716 g          515 t
ORIGIN      
        1 tcttgaagcc gagctgccat tgccattacc attgggagaa acgtagactt gccgtttcgg
       61 tgtgaaaacg gagtaccagg cagggcgatt gtcgaccgtg aaagtggcgg tggcctgggc
      121 gctgttgttg gaggtgtcgg tagcgatggc cgtgacgtta tggctgccat tgctaatttc
      181 ggtcgtatcg aaagcgattc gatagggggg tgtcgtgatg gccgcgccga agttgtttcc
      241 atccacttga aattgaacgc gagcgatggc gaaattgtct tctgccgtgg cgctgagaag
      301 aatcgcgttg ccgaccgtta cgttattgtc cggatgaacg aaggccaccg tgggagcaag
      361 cttgtctcct gccgcattgg cgaccatcac cgtgatggag cttttgctgg agttgcctgc
      421 attatcccac gccgtagcgg ttacggtgtg gttgccatcg gccaccgtgg acgtatccca
      481 tagggcaaag aacggactcg tcaggctttg ggtatgcgct gtagcaatat gcgtgtttcc
      541 cacggaaaag tcgactttgg tcacgcgaat gttgtccgtg gcggtagcgc gaagttggat
      601 ttttccggat aagggtgcgc ctcccaccgg ttggtgtagc gtgaccgtgg gcgcaatcgt
      661 atccggtccc agatacttgg agacatccac cccaatgagg ttgctgcttc cttccttcaa
      721 cccaaggtaa gctgtcaggc ccgagatcgc caccgaagta atgcgaccgt tatcccccgc
      781 cttgtagcct tcgaggcgta acgccgtcgg accatgggat ccgtcgatga cctcgaagtt
      841 ggtcggagcc ctggtctcga gtccggcaaa aagataagag cccgaggctg cgccaccaaa
      901 acttcgcgac gacaggccca cggaggtagg agacttgacg ttaggcaacg cgctcagatc
      961 aaagaccccc acttcccggt catcaaaccc atgatactgg ccgagaaagg cgtaatcgcc
     1021 aatcgtttcg acgtaatatg cccgaatggt gcgcccctca tgggtggata cgacatgggt
     1081 gggattggct ggcgaagaga tattccagac gtgaagaatt gctgcttcgg caggaatcga
     1141 attggagact cccagcaggt aatttttata gatctttagg ccgaaaatcc gtgtatcatt
     1201 gagaagttgc ccttgtaagg acgggctcga tggattcgcc acggagacga tgcgaagacc
     1261 gcggttcgga ttgtcgcccc ctggcgcggc aacacccaca tatgcggttg tgccggagat
     1321 ggcgatggcg gcgacatcga cgccaagttc gagggaacct gccaccgaag gactgctcgc
     1381 gttcgatacg ttgacgatgc gaaactcagg gcccgattgg ttgacggtac cggcgtatac
     1441 cgtattccct tggacataga tagccgtgac ctcagccccc agcgatattt ggcccaacac
     1501 ggttggggtg cctgaaccgc tgatgtctac cacggaaaga ccggattgtc ctccaacgta
     1561 aagacgattg ccggaaacag ccagagcatg gacaggcccg cttccaatat tcgtgctgaa
     1621 acgtgtggaa aggggagccg cctgcgccgt gccgacaaaa cacaatagca ttaggatgat
     1681 catcaactgg atccgaatca tggtaccttc ctccgtttgc cgctgcggaa agtggatggg
     1741 cacgccaata gtgcccatgg tcgcatacat gaacttatga gagtagagag aaccggaaaa
     1801 gaatacaacg acaaaatgag acgcggctta tgacgatcat tcgtgccagt gccgcaaacg
     1861 ttcaatcgta ttgtttgttc acggttgtgg tgttgggatt tcgaactacc taccttgcac
     1921 gacggaacga aggaaagcca tggggaccac cataagcgct tccacaagag aggatggaga
     1981 cctcgacggc gaggcgctac gttggccgat gtgctcaacc gcaagggggg agtttggtcg
     2041 cgcgaatccg tcttcgttcc gggctacatt cggtactgta ggggcgggtt tgaaacccgc
     2101 ccgccatgtg cgattgtcga gatgtcgaaa tcccccgaga acaaagaggg gatggctcag
     2161 cccgtctgga cgttcagatt tccgctccct cttaccctct tattaaacgc ctgtgcccct
     2221 gcgcccccgg ctcttccggc ctttccggaa ccacagggga cttcgcctcg gaaacgtgct
     2281 ttgcggcgct tcgggtaacg gcataaagcg agaggaacgc cgtttcaatc tgcgattgac
     2341 gacgccccag gaatcgtccc gctgctccca gcaactcgga agtggagtcc cggcacccca
     2401 aaggaggctc cc
//
LOCUS       DAOSOR010000737         2405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_43963_length_2405_cov_3.934043, whole genome shotgun sequence.
ACCESSION   DAOSOR010000737 DAOSOR010000000
VERSION     DAOSOR010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43963_length_2405_cov_3.934043"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PK710_24300"
     CDS             complement(<1..592)
                     /locus_tag="PK710_24300"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOT12925.1"
                     /translation="MADFVHLHVHTQYSLLDGAIRLKAEMDKPLEERVDLVGLVRRHG
                     MKAVAITDHANMFGAIGFYKACLAEGVQPILGCEVNVKRDTGAWGAAAERAPVDHLVL
                     LAASEKGYHNLVRIVSLGHVQPASDVGPSVAIETIAANASDVVILTGCLGGRVAQSVL
                     HHGPEAADPALAELRDIADPGHLFVELQHHGFPEQPV"
     gene            879..2012
                     /locus_tag="PK710_24305"
     CDS             879..2012
                     /locus_tag="PK710_24305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12926.1"
                     /translation="MLHTRRSTLLFSALAIGLLAFAGACGGDDGGDDGGGNNVTPDPP
                     CEDCAVPPERPSAGVPGDGDGTVLAMNKLFLGNTDRSGVASNTAWKQFGFDIDGYKST
                     KTSSNHCKVYGKANPSNVKTDGVNGIDNSFGANIIPVITVASADAAQKIEQALKNGSF
                     TLILEVKAIGGGKDYVNLPAALYAGASMVDDAGDPMTPNWDGNDEWPVYCELMQDCKA
                     SGTTQFPDAKSTVTFPSSYMTGGTWVSGDRGTVKLSLSLQGYALDLEINKAVITADMS
                     GNPPTSATNGVIAGVLEAQKLVDSIAKVGGSISTSLCGDTPAFQSVRQGILEAADIMK
                     DGTQDPSAVCDGISVGLGFEMKAVKLGKVLDKRPEGGNPCDGS"
     gene            2188..>2405
                     /locus_tag="PK710_24310"
     CDS             2188..>2405
                     /locus_tag="PK710_24310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12927.1"
                     /translation="MGRKLAASLIAVLFMGCTIHAQTGSPQEPAQPAPPPPPPPPAAT
                     ENPAEPAPAPATPTTSGVKQEGGKLKMPG"
BASE COUNT          546 a          751 c          629 g          479 t
ORIGIN      
        1 gcacgggttg ctcgggaaaa ccatggtgtt gaagttccac gaaaagatga ccggggtctg
       61 caatgtcgcg aagctccgca agcgccggat ccgctgcctc cgggccgtgg tgcaacaccg
      121 attgggccac ccgtcccccc aaacatcccg tcaatatcac gacatccgag gcattggccg
      181 caatcgtctc gatcgccacg ctcggtccca catcgctggc gggctgcaca tgacccaacg
      241 acacgatacg aaccaggttg tggtatccct tttccgatgc cgctagcagc accaaatggt
      301 ccacgggtgc tcgctccgcg gccgcccccc aggcgcccgt gtcccgcttc acattgacct
      361 cacagccaag aatcggttgc acgccctcgg ccaaacaagc cttataaaac ccaatcgccc
      421 caaacatatt ggcgtgatcc gtaatcgcca ccgccttcat cccgtggcgt cgcaccaacc
      481 ccaccagatc cacccgctct tccaacggct tgtccatctc cgccttgagg cgaattgcac
      541 cgtcaagcaa cgagtactgc gtgtggacgt gcaaatgtac gaagtcggcc atcccttcga
      601 cctagccaca gtcggccgaa gagtaaacgg catctggacc ctatcttgcg aatcgtgaca
      661 aatcctaacc tgcgcctcta tatgctactt ttccctaccc acaccatcaa cgccttgttt
      721 ttgcgaaact cgcaaaagaa attcgatcta ttggacattc cccaggttgc tcatgctatg
      781 catgagcctt gcctcaatgg cttgtcctgc tatcacgatt acatccgcga gttcgctact
      841 cgccaaccct tcaccaaccc tctaggaggc taggtagaat gcttcatact cgtcgatcca
      901 ccttgctatt ttccgctttg gctattggtc ttctcgcctt cgcaggcgct tgcggcggcg
      961 acgacggcgg cgacgacggc ggcggcaaca acgttacccc cgatcctcct tgcgaagact
     1021 gtgctgtgcc tccggaacga ccgtcagcgg gagtaccggg agacggggat ggcacggttt
     1081 tggccatgaa caagctattt cttggtaaca cggaccgcag cggtgtcgcg agcaataccg
     1141 cttggaagca attcggcttc gatatcgatg gctataagtc gaccaaaaca tcgtccaatc
     1201 attgcaaggt gtatgggaaa gccaatccat ccaacgtcaa gactgacggc gtcaatggca
     1261 tcgataattc tttcggagcc aacatcatcc ctgtcatcac agtcgcgagt gccgatgcag
     1321 cccagaagat cgaacaagcg ctcaaaaatg ggtctttcac gctcatcctc gaggtcaaag
     1381 ccattggtgg tggaaaagac tatgtgaatc tgccggcggc tctgtacgcg ggcgcgagca
     1441 tggtcgacga tgctggtgac ccgatgactc ccaattggga tggcaacgat gaatggccgg
     1501 tgtactgcga attgatgcaa gattgcaaag cgtctgggac gacacaattt cccgatgcga
     1561 agtcgaccgt tacgtttcct tcgagctata tgacgggagg aacgtgggtt tcgggcgata
     1621 gaggcacggt aaaactctcg ctatcgctcc aaggctacgc gcttgacctg gagatcaaca
     1681 aggcggtcat cacggcagat atgtcaggca atccgccgac ttccgcaacc aatggcgtca
     1741 tcgccggcgt gctcgaagcc caaaaactgg tcgattcgat cgcgaaggtg ggcggcagca
     1801 tcagcacgag tctgtgtggt gacactcccg cgttccaaag cgtcaggcaa ggcatcctcg
     1861 aagccgcgga catcatgaag gatggcaccc aggatccttc cgcggtttgc gatggcatct
     1921 cggtcgggct tggattcgag atgaaagctg tcaagctggg aaaagtgctg gacaagcgac
     1981 ctgagggggg caatccttgc gacggctcct gagccaaagc tagcctgatt gcgcacaaaa
     2041 cccttttctg ctccataaag tagagctaca tagttttttc aatgaacata gccggggggc
     2101 aacccgtcca acccggcgcg ttcgctcgcc attagccacg aacgcgatat gccgtcttga
     2161 aggcaacgtc gaaggaggaa agacatcatg ggtcgtaagc tcgctgcttc gcttattgca
     2221 gtccttttca tgggctgcac catccacgct caaactggct ccccgcaaga acccgcgcaa
     2281 cccgcgccgc ccccgccgcc tccgccgccg gccgccaccg aaaatccagc ggaaccggct
     2341 cccgcccccg ctacccccac aaccagtggc gtcaagcaag agggcggaaa actcaagatg
     2401 cccgg
//
LOCUS       DAOSOR010000738         2399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_44120_length_2399_cov_5.194539, whole genome shotgun sequence.
ACCESSION   DAOSOR010000738 DAOSOR010000000
VERSION     DAOSOR010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44120_length_2399_cov_5.194539"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..2163
                     /gene="htpG"
                     /locus_tag="PK710_24315"
     CDS             244..2163
                     /gene="htpG"
                     /locus_tag="PK710_24315"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOT12928.1"
                     /translation="MTDTEAKEKDAAQELPFQAETKQVLHLMVHSLYTNKEVFLRELI
                     ANASDALDKVRFLAVTDHAMAERVGPPEIIIQVDRESKTITVEDNGIGMTRQEAIDNL
                     GTIAHSGTVEFLKKLQTDKDETRANLIGQFGVGFYSAFIVADRVDVESRSARDPDAEP
                     VLWRSSGEGSFFITKGERDRPGTKVTVHIKGDENDDLLSDWRIEALIKKYTDFVSHPI
                     KLGEKAVNQTTALWMRPRSEIKQEQYDEFYAHVHGAMEPGTPLERIHFSADAPIQYNV
                     LLFIPSKAPVDLLMDTKKKGIRLYAKRMFVMDGCERLAPPYLQFLRGVVDSEDLSLNV
                     SREILQDDRKLDVIKKAITKQTLKALQTMAEEQPEKYQNFWKEFGRLLRLGVSSDPAN
                     QESIGKLLRYPSTATTDEELTSLDAYIGRMKEGQKAIYYLTGPSLKALRSSPHLEVFR
                     RKGVEVLLMHEAVDEWVVGGLFTYDKKNFESIAHGTVDLSDLPNVDGQQDDQSEPSLD
                     AQMQACVKRIEEVLGDRVKQVRTSSRLTDSASCLVSDEGDVSPQMERVMRMLDREVES
                     PKRILELNPKHELVKNLALMVAAEPESEDVITFSELLLDQAMLAEGVVPDPSGLLARM
                     QRVATAASQRTRQSR"
BASE COUNT          556 a          546 c          768 g          529 t
ORIGIN      
        1 gggggtagat gcaggcatgg gatgaccgac gagaggggta ggcggtttga agctcgggtg
       61 atgggctgat gggggttggg ggattcgggg ccatgagcta tcgttggttt tcgggatatt
      121 tgggtggtgg cgcgaaggcc aggcgtatcc gattggtcct tcggatggct gccgggtctt
      181 gccattgtcg aaagttgggt taacgaagct ccgaatccca ggcgagtgta tggaggcaga
      241 cgcatgacgg atacggaagc caaggagaaa gacgctgcgc aggaattgcc ttttcaggct
      301 gagaccaagc aagtgctgca cttgatggtt cattcacttt acaccaacaa ggaagtgttt
      361 ttgcgagaac tcatcgcgaa cgcttctgat gcgttggaca aggttcgttt ccttgcggtc
      421 accgatcacg cgatggccga gcgtgtgggg ccgcccgaga tcatcatcca agtcgatcgg
      481 gagtccaaaa ccatcaccgt ggaagacaat ggtattggca tgacccggca ggaagcgatc
      541 gacaacctcg gaacgatcgc ccattcgggt acggtcgagt ttttgaaaaa gcttcagacg
      601 gataaggatg agacgcgcgc caacctcatc ggtcaatttg gtgtggggtt ctattccgcg
      661 ttcatcgtcg ccgaccgggt ggatgtggag tctcgatccg cgcgggatcc ggatgcggaa
      721 ccggtgttgt ggcgttcgtc cggggaaggc tcctttttca taaccaaagg tgagcgtgac
      781 aggcccggca ccaaggtgac ggttcatatc aagggggatg agaacgacga tttgctatcg
      841 gattggcgta tcgaagcgct catcaaaaag tatacggatt ttgtcagtca ccccatcaag
      901 ctgggcgaaa aagctgtcaa tcaaacgacg gcgttgtgga tgcggcctcg gtccgagatt
      961 aagcaggagc agtacgacga gttttatgcg cacgtgcatg gggccatgga gccgggcact
     1021 cccctcgaac gtattcattt ttccgcggat gcgccgattc aatacaacgt gttgctattc
     1081 attccgagca aggcacccgt ggacttgttg atggatacca agaaaaaggg cattcggttg
     1141 tatgcaaaac gaatgttcgt catggatggc tgcgagcgtc ttgcgcctcc ctatctacaa
     1201 tttctccgtg gtgtggtgga ttccgaggac ctttctctca atgtgtcgcg agagattttg
     1261 caagacgatc ggaaactcga tgtcatcaag aaagccatca ccaagcagac gctcaaagcg
     1321 ctgcagacga tggctgagga acagccggag aagtatcaga atttctggaa ggaattcgga
     1381 cgcttgttgc gtcttggggt atcttccgat cccgcaaacc aggagtcgat tggcaagctg
     1441 ttgaggtatc catcgacggc aacgacagac gaagagctga cctcgttgga tgcgtacata
     1501 ggcaggatga aggaaggaca gaaggcgatc tactacttga ccgggccgag tttgaaagcg
     1561 ttgcgaagca gcccgcacct cgaggtattt cgtcgaaaag gggtggaagt cctgctgatg
     1621 cacgaggcgg tcgatgagtg ggtcgtgggt ggattgttca cctacgacaa gaaaaatttc
     1681 gaaagcatcg cccatggtac cgtcgatctt tcggatctac cgaatgtgga tggtcagcaa
     1741 gatgatcaaa gcgagccttc gttggatgcg cagatgcaag cttgtgtgaa acgaattgag
     1801 gaggtgctgg gcgaccgggt caagcaagtg cgaacctctt caaggctgac ggatagcgcg
     1861 tcgtgtttgg tttccgacga aggtgatgtg agtccgcaga tggagcgagt gatgcggatg
     1921 ctcgatcggg aagtggaatc accgaagcgg attttggaat tgaatcccaa gcacgagctt
     1981 gtaaaaaacc tggccttgat ggtggctgcc gagcctgagt cagaggatgt gatcacgttt
     2041 tccgaactgc tattggatca agcgatgttg gccgaggggg tggtacccga tccctcggga
     2101 ttgcttgcga gaatgcagcg tgtggcgact gccgcaagcc agcgaacccg ccagtctcga
     2161 tgaaccaacc catgcttcgc gcatcgactg atttgccctc tgtcgatgga aaaatcctca
     2221 tcccatcatc gatgcgtgtt tgttctcccc gttgaatgac ccatggaagt aggatggtgc
     2281 cgatgtcagg atcgggacgg atagtgatcg cgggacgtta cgaactgctg gaagtagcag
     2341 gatcgggagg gatggcggcg gtatggcgag gagtgatgcg cgagggcgac caccaagtg
//
LOCUS       DAOSOR010000739         2399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_44122_length_2399_cov_3.723549, whole genome shotgun sequence.
ACCESSION   DAOSOR010000739 DAOSOR010000000
VERSION     DAOSOR010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44122_length_2399_cov_3.723549"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="PK710_24320"
     CDS             complement(<1..1085)
                     /locus_tag="PK710_24320"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOT12929.1"
                     /translation="MAYDIPSMQMPRIAGKALRALVKAVETPGLGHAVRVQMLSNLGF
                     DVFRNTPAHGSIAFGPALPSLDRQASAPVSPSLLESMLQSDVHESGFRFETVADYRRA
                     YLDGATTPTQVAERLADAIAQSDQSDPPLRVFITFRREDLVRQAEQSTRRYAEGRPLS
                     VLDGVPVAVKDEVDMLGYPTTVGTRRLGKEAACQEATAVARLRAAGALLIGKANMHEL
                     GIGVTGINPHHGAARNPYDRSCITGGSSSGSAAAVAAGMGPLALGADGGGSIRIPASL
                     CGLVGLKPTFGRISEKGAAPLCWSVAHLGPIGATAMDATIGYALMAGKDEADIFSLRQ
                     PEVEVEGIQEQNLEGYKLGVFRPWFEDA"
     gene            complement(1211..1741)
                     /locus_tag="PK710_24325"
     CDS             complement(1211..1741)
                     /locus_tag="PK710_24325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12930.1"
                     /translation="MHVLVAIHPVFEEGSALELTKRTHNLGHWSETMNKMTYGIVISL
                     GTALLFTGCCKKGDDEQQPTSPIIDTEPEPSGATITGSSALEKLKAKGYTPITDPVVG
                     ENGGIKSTAVVLKPDLITVNIIEYPDANMAKLAASGSASVNDVLTHLSGKSVIRVTCV
                     GKPKDKCEAIMSTIKD"
     gene            1894..>2399
                     /locus_tag="PK710_24330"
     CDS             1894..>2399
                     /locus_tag="PK710_24330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12931.1"
                     /translation="MTCHHREMIRCGWLVGALCFLAIGCGSSEQTGSEPPFDGGVEVA
                     IEGSTNGEAGEEAMSQIDGEVWQDAIYSEVDGQDETIVETCEPIVACAAPLPDFGPAR
                     GFHKTSSKLTAKIGSPRHRGRDLFLLEGQDAWVLGKFAYGLLDDDIKGEEIDVYLLKD
                     CGNTWSLVG"
BASE COUNT          496 a          654 c          693 g          556 t
ORIGIN      
        1 gcatcctcga accacggacg aaacaccccg agcttatatc cttccaggtt ttgctcttga
       61 atcccttcta cttccacctc cggctgccga agagaaaata tgtccgcctc gtcctttcct
      121 gccatcaatg catacccaat cgttgcgtcc atggccgttg cgccgatggg gcccaaatgg
      181 gcaacgctcc agcaaagagg agcggcacct ttttcgctaa ttcgaccgaa cgttggtttg
      241 agcccgacca gtccgcacaa cgatgcggga atgcgaatgg atccgccacc atcggctccg
      301 agcgccaacg gtcccatgcc agcggctacc gctgccgcac tgccgctgga agagccaccc
      361 gtgatgcacg accgatcgta aggattcctc gcggcgccat ggtgtgggtt gattcccgta
      421 accccgatgc caagttcatg catattcgct ttgccaatga gcaaagcgcc ggctgcccgt
      481 aatctggcca ccgccgtcgc ttcctgacac gcagcttctt ttcccaacct ccgagtgccg
      541 acggtcgttg gatagccgag catatccact tcatccttca ctgccaccgg aactccatcg
      601 agtaccgaaa gtggtctgcc ttccgcatac cgccgcgtgc tctgctccgc ttgacgaacc
      661 aaatcctctc gtcgaaacgt aatgaatact cgtagtggag gatcggattg gtcgctttgg
      721 gcaatggcat ccgccaatcg ttcggccacc tgcgttggtg tggttgcacc gtctaggtaa
      781 gctcgccgat agtccgccac ggtttcgaag cgaaagccgg actcgtggac atcgctttga
      841 agcatggatt cgagaaggga aggggagacc ggagcgcttg cctgccgatc caagctggga
      901 agcgctggcc cgaaggcaat cgagccgtgt gccggggtgt tccgaaacac atcgaaaccc
      961 agattcgaca gcatttgcac tcgaaccgca tgcccaaggc caggggtttc aacggccttg
     1021 acaagagcac gaagtgcctt gccggctatt cgtggcatct gcatcgaggg gatatcgtac
     1081 gccatggttc cgcctttcgg cccttatata cgggatctgc cgagaggaaa ccagtcatgc
     1141 ttcacgaaca ggcaccaacc cgttgcgctg tcgatgcgat gcgaaaggaa aatcgaaaac
     1201 gctgcgacat tcagtccttg atggtggaca tgatggcttc gcatttgtcc ttcggttttc
     1261 cgacgcatgt cacgcgaatg accgacttgc cgctcaagtg cgtcagtacg tcgttgaccg
     1321 atgcactgcc cgacgctgca agcttcgcca tgttggcgtc ggggtattct atgatgttga
     1381 ccgttatcaa atcgggtttg agcacgacag cggtgctttt gataccgcca ttttcaccga
     1441 ccaccggatc ggtgatgggc gtgtaccctt ttgccttgag cttctcgagg gccgaggagc
     1501 cagtaatggt tgctccggaa ggttcgggtt ccgtgtcgat gatgggagaa gtcggttgct
     1561 gttcgtcgtc tccttttttg caacaccccg tgaatagcag agccgtgcca agcgaaatga
     1621 cgattccata agtcattttg ttcatagtct cgctccaatg accaaggttg tgagttcttt
     1681 tcgtgagttc gagagcggaa ccttcttcga agacgggatg aatggcaaca aggacatgca
     1741 tgccgtggag cgattttggc cagtggccga tcttgctcga agccgacgtt gcaacgcgtc
     1801 atgaggtgat gtcggtggga gtgagtaggt ttggagggct gactaccggt cggaaaattt
     1861 gcgtttttcg tcttgacccc cccagttcgc ctcatgacat gtcatcatcg tgagatgatt
     1921 cgatgcggtt ggttggttgg cgccctttgt tttctggcca taggttgcgg ttcatcggaa
     1981 cagacaggct ccgaaccccc gttcgatggc ggtgtggaag ttgccatcga ggggagcacc
     2041 aacggggagg ctggcgaaga agccatgagc cagatcgatg gagaggtttg gcaggatgcg
     2101 atctattcgg aagtcgacgg gcaagatgag acgattgtgg aaacctgtga gccaattgtt
     2161 gcgtgtgcgg cgccgcttcc tgactttgga cccgcgcgcg gctttcataa aacgtcttcc
     2221 aaactcaccg cgaagatagg atcgccgcga caccgcgggc gtgatctatt cttgctggaa
     2281 gggcaagacg cttgggttct gggcaaattc gcttacggtt tgttggatga cgatatcaaa
     2341 ggggaagaga tcgatgtgta tctgttgaaa gactgcggga acacatggtc cttggtggg
//
LOCUS       DAOSOR010000740         2396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_44189_length_2396_cov_4.198633, whole genome shotgun sequence.
ACCESSION   DAOSOR010000740 DAOSOR010000000
VERSION     DAOSOR010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44189_length_2396_cov_4.198633"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..1777
                     /locus_tag="PK710_24335"
     CDS             263..1777
                     /locus_tag="PK710_24335"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HOT12932.1"
                     /translation="MPQRPIVRFLVLIGVTSIGCNDLPKEAEGVGTRSMPVVGGVPAE
                     VGDYPSTVSLMMKQGGKWESGCTGTLVAPTVVITAAHCLEDWWGTPLSINEIRIVYGY
                     TNPQTAPSSELRTLASLHPHPNYAPNASTDSDGLGKTNDIGVVVLNEPIANGVTTPIL
                     PENMVDSVLVPGSQVHVVGYGINNINTWASGILYKAITPYIRHISWEMLAGSPGNPDS
                     CNGDSGGPAYMEVGGALYLVGVTSRAWKKSQKMCGDGGIYTIASAHLTWLEGVVGNLD
                     AGVSDITLDVSHLDASESCLPLTSRCHPMTNEGCDSSAGEACKVTNGAMTCQKASNDV
                     QEGGLCDQTSRFCAVGLHCGSTFRCQRVCCSDADCPQGGPCLPISGTIGSLGTCEWVK
                     QDASPDVETDSTTEAAADAMGGAGGAAGQGGMAGQGGVAGQGGMAANGGAAGSGQAAG
                     HAGDGNESRPPKPSAEEDDEGCGCRTAGSRSVGTPAWLAALGLGLLLRRRQKTL"
     assembly_gap    1887..1896
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1897..>2396
                     /locus_tag="PK710_24340"
     CDS             <1897..>2396
                     /locus_tag="PK710_24340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOT12933.1"
                     /translation="DEIKAAFRKLAAKHHPDRNAGDVSANERFKEINAAYQLLSDPKK
                     RMMFDRFGAAGANASGANPFAGGMPFDFDLSGVSLDGIFGDLLGAFGFGGKGDRGDVK
                     KEVTIDFAEAAFGCEKEVTYERAELCERCHGSGAASGADTRTCSACNGRGRIRFQQPF
                     LPMAAE"
BASE COUNT          485 a          613 c          783 g          505 t
ORIGIN      
        1 acaataagtt cgcgattttc aaagttaaat ttggaaatcg accgtcgcaa cccgccccgg
       61 agaacacgga gacgacggaa gaagacgacg gataaacggg ggggtggccg gtcttcgacg
      121 gtgaaatcac ccgcctcggc gtgttcgact tgcacggtgg gcggaatcaa aacacagtag
      181 gagccatcct gacggaaccg cggttcgacc atcgtgggcc gctgccgttc accgaacagc
      241 cctcttgccg gtggagtcgt acatgccaca gcgaccaatt gtaaggtttc tcgtactgat
      301 tggtgtcaca tcgattgggt gcaatgactt gccgaaagag gcggaaggcg tcgggactcg
      361 ttcgatgccg gtggtgggag gcgtgcctgc ggaggtcggt gattaccctt ccacggtttc
      421 gttgatgatg aagcaggggg gcaagtggga gtcgggatgt acgggtactt tggtcgcacc
      481 taccgtggtg attacggcgg ctcattgtct cgaagattgg tggggcacac cgctttcgat
      541 caatgagatt cgcatcgtgt acggttatac caatccccag accgcgccat cttccgaact
      601 gcgcaccctc gcatcgctgc atccgcatcc gaattatgcg cccaatgctt cgaccgactc
      661 tgatgggctc ggcaagacga acgacattgg cgtggtggtg ttgaacgaac cgatcgccaa
      721 tggggtgacg acgccgattt tacctgaaaa tatggtcgat tccgtgctgg taccggggtc
      781 gcaggttcat gtggtcggct atgggatcaa caatatcaac acgtgggcat cgggcatctt
      841 gtacaaggcc atcacgccct atattcgaca tatttcatgg gagatgctgg cgggaagccc
      901 tggcaatccc gatagctgca atggcgatag tggtggtccg gcgtatatgg aggtgggcgg
      961 cgccttgtac cttgtgggag tcacctcccg cgcctggaaa aagtcgcaaa aaatgtgcgg
     1021 tgatgggggt atttatacca tcgcttccgc tcatttgaca tggctcgagg gggtcgtggg
     1081 taacttggat gccggggtat ccgacatcac cctggatgtt tcgcacctgg atgccagcga
     1141 atcgtgcttg cccctgacgt ctcgctgtca tcccatgacc aacgagggat gtgattcgtc
     1201 ggccggagaa gcatgcaaag tcacgaacgg ggcgatgacg tgtcagaaag cctccaacga
     1261 cgtgcaagag ggcggtttat gcgatcagac ctctcgattc tgtgccgtgg gattgcattg
     1321 tggttcgacg tttcgctgtc agcgggtttg ttgctcggat gcggattgtc cgcagggagg
     1381 cccttgcttg ccgatttctg gcacgattgg ctcgctgggg acatgcgaat gggtcaagca
     1441 ggatgcttcc ccggatgtcg agactgattc cacgacggaa gcggcggcgg atgcgatggg
     1501 aggagcgggg ggggctgcgg gccaaggcgg tatggcggga caaggtggcg tggcaggaca
     1561 aggtggcatg gcggcaaacg ggggagccgc gggttccggg caagcagcag gccatgcggg
     1621 cgatggcaat gaatcccggc cccctaagcc ctcagcggaa gaagacgatg aaggatgcgg
     1681 ttgccgcacg gcgggctctc gttcggtcgg tacgcccgcg tggctcgctg ccctcggttt
     1741 ggggctgctg ctacgacgtc gtcagaaaac tctctaatct ttccttggga actacgtctg
     1801 tgcgtcgggc cacggctcgt gctatggcgg aagggtgcaa gacccgtatg aagtgcttgg
     1861 tgtgccgcga gatgcgaccg ccgacgnnnn nnnnnncgac gagatcaaag ccgcctttcg
     1921 caaattagcc gcgaagcatc atccggatcg caatgcgggt gatgtttccg ccaacgaacg
     1981 gttcaaagag atcaacgcgg cgtatcaact gctatcggat ccgaaaaaac gtatgatgtt
     2041 cgaccggttc ggcgccgccg gagccaacgc atcgggcgcg aatcccttcg cggggggaat
     2101 gccattcgac ttcgatttat cgggcgtgag tcttgacggt atcttcggcg atcttcttgg
     2161 agctttcggt tttggcggaa aaggggatcg aggggacgtc aaaaaagagg tcaccatcga
     2221 ttttgccgaa gcggctttcg gatgtgaaaa agaggtcacg tacgagcgag cggaattgtg
     2281 cgaacgttgc cacggttctg gggcggccag tggagcggat acccgaacct gttccgcatg
     2341 caatggccgt ggccgtattc gctttcaaca gcctttcctt cccatggcgg ccgagc
//
LOCUS       DAOSOR010000741         2395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_44229_length_2395_cov_3.634188, whole genome shotgun sequence.
ACCESSION   DAOSOR010000741 DAOSOR010000000
VERSION     DAOSOR010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44229_length_2395_cov_3.634188"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PK710_24345"
     CDS             complement(<1..422)
                     /locus_tag="PK710_24345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12934.1"
                     /translation="MSTSTAPGYLRLIGFSGTNKVMAGELSRLTTRAGISHCLPEPRK
                     DGLGSLVYPFDPHIAALAVRYHRTSTRVLWDLYESRATRLEPLYDDLVRQIRDDDRGW
                     GQSGYGISVTTHGVQEFAAGERQIVGTVKNALIDGSKQR"
     gene            complement(517..2094)
                     /locus_tag="PK710_24350"
     CDS             complement(517..2094)
                     /locus_tag="PK710_24350"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT12935.1"
                     /translation="MPLKFLGQDTGVLPRIDRYELIAELASGGMASVYLARLVGMGGF
                     ERFFAIKRLHPHLGHEEAFVSMFLDEARIVAGIRHQHVVPVLEVGASDHGYYLVMEYI
                     EGENLGTFLTQAIETKSPIPPNVVVRILLDVLAGLHAAHEHVDADGHPIGLVHRDVSP
                     QNILIDTNGVARIADFGVAHATSRLSSTRSGQLKGKVAYMAPEQARGEGVSPRSDVFA
                     AAIVLWESLTSRRLFLANNEAVTLNRLLFEPIPELEEVAPSIPRGIARVLARALQRDP
                     AKRYASAADMADELQATARASGWLGSASDVKYYVRNVVGVAMERRRASIREHLSRVSS
                     ESSGTANAPGMLERPDIPFSKPSNSSVSSATMAVSTASSITSSSDTHSETPTASRKRS
                     SSLLVAGLGFGLLIVVAVFFALNTNRSLNQGASMPSASTVLPTTSSTASLPITPPSFF
                     SHTISELATTSAQPSASYPTTPIASTRSTRPTSGNGKTTTASHPSASAKSPSEPTTAT
                     ATPTATSLDDLRQNPYR"
BASE COUNT          503 a          551 c          827 g          514 t
ORIGIN      
        1 cgttgcttcg agccatcgat caaagcgttt ttgacggtac ccacgatctg ccgttcgcct
       61 gcggcaaatt cttggacgcc atgagtcgtg acgctgattc catatccaga ctgtccccaa
      121 ccacggtcat cgtctcggat ttgacgcact agatcgtcgt acaagggttc gagacgagtg
      181 gctcgactct cataaaggtc ccacaacaca cgggtacttg tgcgatggta ccgaacggcg
      241 agggcggcga tatggggatc gaatgggtat acgagcgagc caaggccatc tttgcgaggt
      301 tccggcaagc agtgggagat gccggctcgg gtggttaggc gtgacagttc gccagccatc
      361 accttgttcg tgccgctgaa accgatcaac cgaaggtaac caggtgcggt cgacgtggac
      421 atgatcgtga gagtatcgca attgggtatt gatccaagaa gtgtgaaaac gacggagatc
      481 cgtgctcatc tctgaggtga tcgcaagcag gtatctctag cgatatgggt tttgtctcaa
      541 gtcgtcgagg gaggttgcgg tgggggtcgc cgttgcggtg gtaggttcgg aaggagattt
      601 tgcagaggcg gaaggatggg aggctgtggt tgtttttcca tttccggagg ttggtcgggt
      661 tgatcgtgtg gatgcgatcg gcgtggttgg gtacgaagcg gaaggctggg cggaagtcgt
      721 agctagctcg gaaatcgtgt gcgaaaagaa cgatggtggt gtgatgggca gcgaggcggt
      781 actgcttgtc gtgggcaaga ccgtggaagc gctgggcatc gaggctcctt ggttgaggga
      841 gcgattggtg ttgagtgcaa aaaagacggc gacgacaatc aataggccga atcctaggcc
      901 tgccacgagc agcgacgatg agcgcttgcg gctggcggtc ggagtctcgg agtgggtatc
      961 ggaagaggac gtgatggatg aagcggtcga tacagccatg gtggcggacg acacgctgga
     1021 attggatggt ttcgagaatg gtatgtcggg gcgttcgagc atgccgggag cgtttgcggt
     1081 gccactcgat tcgctggata cacgagacaa gtgttcccgg atcgatgcac gtcgacgttc
     1141 catggcaacg ccgaccacgt ttctaacgta gtatttcacg tctgatgcgg agccgagcca
     1201 tccgctggcg cgagcggttg cttgcagttc atcggccatg tccgcggcgc tggcgtaacg
     1261 tttggcgggg tctcgttgca gggctctggc gagtacgcga gcgataccac ggggaatact
     1321 gggggcgact tcctcgagtt cggggatggg ttcgaaaagc aaacggttga gggtgacggc
     1381 ctcattgttg gcgagaaaga ggcgacgact cgtcaaactt tcccacagga cgatggcggc
     1441 ggcaaaaacg tcggagcgag gggaaacgcc ttctccgcgc gcttgttctg gcgccatata
     1501 agcaactttt cctttgagtt gacccgaacg agtgctgctg agtcgcgatg tggcgtgggc
     1561 gacgccaaag tcggcaattc tggcgacacc attggtgtcg atgaggatgt tctgtgggga
     1621 aacatcgcga tggacaaggc caatcggatg tccatcggca tcgacatgct cgtgagccgc
     1681 gtgcaatcct gcaagcacat cgagaaggat acgcacgacg acattgggcg ggatgggcga
     1741 tttggtttcg atggcttggg tgaggaatgt gccgaggttt tcgccttcga tgtattccat
     1801 cacgaggtag tagccatgat cggaagctcc gacctcgagg acaggcacga catgctggtg
     1861 acgaatacca gcgacgatgc gagcctcatc gaggaacatg ctgacgaaag cttcttcgtg
     1921 accgaggtgg gggtggagtc ttttgatcgc gaaaaagcgt tcgaaaccgc ccattccgac
     1981 gaggcgggcg aggtacacgg aggccattcc tccgctagca agctcggcga tgagttcgta
     2041 gcggtcgatg cggggcaaga cccctgtgtc ttggccgaga aacttgagcg gcattcgacg
     2101 gatctcgtgt caacgtacta gcggatgaag acttcttaaa acggtacacg actcgagcga
     2161 ggttgtccaa gaagggcatt ggcaggcagg gaggaatcgt ggaggcaggc gagcggatcg
     2221 aacgctattc cgatggtggg atggactacc aggtgccgat gttattggcg cgtgcttgtt
     2281 gacgggtcag tccacggttt gccgcgtcat tttttcggtt accggaggcg gtggtgtaga
     2341 tttcgtcgag gttggcggtg atatcgaaga acagacgagc atcggggctc cacag
//
LOCUS       DAOSOR010000742         2388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_44403_length_2388_cov_4.147878, whole genome shotgun sequence.
ACCESSION   DAOSOR010000742 DAOSOR010000000
VERSION     DAOSOR010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44403_length_2388_cov_4.147878"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PK710_24355"
     CDS             <1..443
                     /locus_tag="PK710_24355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12936.1"
                     /translation="LARRTDHLLRNPSTRYLIIGAPTGGAMADAAFLVDNLGRNGLKP
                     SAIVLNRAESEPPLCEQRVMQAISDALFDVPEQHLLALRNTLETMQNEHQLRGQAATR
                     TMSALQDIAPAGTRVLRLPFIHRSEPSAIVIALADAWLATGSDL"
     gene            complement(539..1309)
                     /locus_tag="PK710_24360"
     CDS             complement(539..1309)
                     /locus_tag="PK710_24360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12937.1"
                     /translation="MTRIDYWKAAFVTGCSVLAFVAVHLGHTDDADAAKIRKKPPLQP
                     LASRSAPSPATSVPPSTPGASAPAPTVDPTPAIPYPTSGLVCLYGEQRDPESQKIRCL
                     APEELSPPRLVYTDTRALAEQLGMYNPATTPPPAGITEGPEGPEPENPKARVVTVSFE
                     NGVVGGALKNLRSHTDEFASCLDQHGGLRVPSARLKLMFFVRSNQKPSEMIVASARNV
                     PTPIVRCVRKVIESNAIGRPSTDAVGVTVLIELKNPGS"
BASE COUNT          514 a          631 c          727 g          516 t
ORIGIN      
        1 gactcgcacg acgtaccgat catctgctgc gcaatccttc gactcgctat ctcatcattg
       61 gtgcgcccac cggtggtgcc atggccgatg ccgccttcct cgtcgataac cttggacgca
      121 atggtttgaa accatccgcc attgtgctca accgcgcgga aagtgaacct ccgctgtgtg
      181 aacaacgagt gatgcaagcc atctccgacg cccttttcga tgtgccggaa caacatctgc
      241 tcgcgctccg gaatactctc gagaccatgc aaaacgaaca tcagcttcga ggacaggccg
      301 caactcgaac catgtccgcc ttgcaggaca tcgcccccgc cggaacccgt gtccttcgac
      361 ttccgttcat ccaccgttcc gaaccaagcg ccattgtcat cgcgttggcc gatgcatggc
      421 tcgcgaccgg ctcggatctt tgaggtgatc acgggcctga gtccacgctc ccacgataac
      481 cgagggcttg ttgaaataat caataaatac gtatagttat tagcaggtgg cgcctgtctc
      541 agcttcccgg atttttcaac tcgatgagga cggtaacccc gacggcatcc gtggaggggc
      601 gtccgatggc gttgctttcg atcaccttgc gcacgcaacg gacgatggga gtgggcacgt
      661 tacgcgcgct ggccacgatc atttcggaag gtttttggtt ggagcgaacg aagaacatga
      721 gtttgagcct tgcgctcggc acgcgcagtc ctccatgttg gtcgagacag gaagcgaatt
      781 catcggtgtg ggaacgaagg tttttgaggg ctccgcccac cacaccgttt tcgaaggaga
      841 cggtgacgac gcgtgctttg gggttttctg gctcaggccc ttcagggcct tccgtgatgc
      901 cagcaggcgg gggcgtggtg gcggggttgt acataccaag ctgttccgcc agggctctgg
      961 tgtccgtgta gaccaggcgg ggaggggaga gttcttccgg cgcgagacat cggatttttt
     1021 gagattccgg atcccgttgt tcaccataaa gacacacgag gcccgaagtg ggatacggaa
     1081 tcgctggcgt tggatcgacg gtgggggcag gtgcgctagc tcctggagtc gaagggggga
     1141 cggacgtggc aggcgacggc gcggaacgtg atgctagcgg ctgaagcggc ggtttttttc
     1201 gtatcttcgc ggcgtcggcg tcgtcggtat ggccgagatg gacagcgacg aaagcaagaa
     1261 cgctgcatcc ggttacgaag gcggctttcc aataatcaat ccgggtcacg acgtgcagct
     1321 tgttccgaaa tgggccggtc gtcgaggggt tccttgccat tggggcggca tgaacgaaaa
     1381 gcgcggcggg aaggctttca cgagtgggtg gaatgccaac cctccacgac gacaaagccg
     1441 tcgtcggacg aaagaaatac caggggaaaa tgaggggatg gttcagaggc tggctgggaa
     1501 gcatgcggtt cggcaagcca tactttcgta caaggacctt gtcggtttcc tgttcggaga
     1561 gcagcccctg cggggcacgt ttctatccag ccactcgagc actccttgct caagcgggtg
     1621 ctgccgtgcg tgtgctggtg cagcgggtcc gtcgccgggt gggacggggt tgaggtagca
     1681 cggctcgccg gtttgctcga aggctctgac ggcctcggca aatctggggg tgtgtcgggt
     1741 tgcaaagcat tacggcatgc atgagaaggg atgacacggt gcgatttctg gagcccgtag
     1801 ccatacgcgg caaatcagca catatcctcc cgtcgccaga gactacactg gcgatgatgt
     1861 gttgtgtacg agcgagtaac ctgcttgcgc tcgcgagtgg ctcttacccg gtccacaggc
     1921 gattggctcg aagcctacat tggcgctctc aaacaagaac gctaagcaat cgacgacatg
     1981 gatagatggg tccatgcttt tcaaggttga cataagcggg ccatgatttg aaactcctta
     2041 ttagcattcg aatagcgacc tcatccaacg ctgaaagatc ccctcatatt ttcctgaaaa
     2101 acaagggata tgagagacaa tcccctaaac catgaccttg acattgacat tgacctggtc
     2161 cttgacatga acttgaacgt gaaattggac ccaaaacctt gcgtttaacc tggtcttgaa
     2221 ccttagcaat tcctggccga gtggtgaagg gtacgacgtt ccggcagtcg acaacaaggg
     2281 aaatactccg ggaacaatga ggggatggct caggggtagt gtatgcggtg attgaaacta
     2341 gtactacaga tcctttcttc gttccacatt aggaccgggg cgtaagaa
//
LOCUS       DAOSOR010000743         2386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_44470_length_2386_cov_3.434577, whole genome shotgun sequence.
ACCESSION   DAOSOR010000743 DAOSOR010000000
VERSION     DAOSOR010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44470_length_2386_cov_3.434577"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="PK710_24365"
     CDS             <1..1292
                     /locus_tag="PK710_24365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12938.1"
                     /translation="QPLRDTPELFSALKSSASRSPRPRPSSALATADARLGGGLFGGG
                     SAQQARVEHRDRDQDLFGAAQHPVEEEVSTSAPVNPMEKPAVPKFTAERGENSVLFTI
                     DTIMAVGTQSAKRPSAFPEEEKIDFRKLASVVPPPMDSGDFGASFESPPSFGGPALGA
                     LAAPTLPTSIEPEPMPEPVAEPEAMYPYDAPAKSNKGLIIGLGAAVVVLLLVTLGLGG
                     YFLFGSSEEPVAANTTTPTAEAVTAPEDKTDDKSERTTDNEKKDDGAQDAKDKKEDGA
                     DKDKKDDKKDEATAKADATGKVGGPLPVGTGTAKAVADPVKSDPKADSKKDDAKPADV
                     PAASADRKFSRSAAVSALNAAAGAASGCKKPDGPTGRGRVSVTFAPSGRVTTAVVNGP
                     PFAGTSVGGCVAAAFRRATVPPFDGSPVTVHKSFSIN"
     gene            complement(1318..>2386)
                     /locus_tag="PK710_24370"
     CDS             complement(1318..>2386)
                     /locus_tag="PK710_24370"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT12939.1"
                     /translation="APTGRASRRMSETTGKDAYTVHRLLEFDPRGGRFTRHEKKPIEA
                     GAIIVDESSMLDLTMSFALIRAIATGTRLVLVGDVDQLPSIGAGAVLRDIIDSRAIPC
                     VRLHKVFRQASQSRIVANAHRIREGLEPQTPEKGDLASDFFIAPAKDAHDAANKVLLL
                     VQERIPRQFHLDPVRDVQVLTPMRRGPAGIEALNERLQHALNPPHIPQRPFPMNLRMG
                     DKVMQLRNDYTRDVFNGDLGFVTHTDPNAEQAVVEIDGRPIVYDRPHLDELRLSYACS
                     IHKSQGSEYPAVVIPWVTAHFIMLSRNLLYTAVTRGQRLVVLVAEPRAIQLALSQTRR
                     EQRYSNLMQRLAAAVQQQPLV"
BASE COUNT          490 a          622 c          778 g          496 t
ORIGIN      
        1 ggcagccgtt gcgtgatacg ccggagcttt tctccgcgtt gaaaagcagt gcttccagat
       61 ctcctcggcc tcgtccttcc tcggcactcg caacggccga cgcgcggttg gggggaggac
      121 tttttggggg aggttcggcg cagcaagcgc gcgtggagca tcgtgaccga gatcaagatc
      181 tgttcggcgc ggctcagcat cccgtcgagg aagaggtatc gacgagcgct ccggtcaacc
      241 cgatggagaa gccggccgtg cccaagttta cggcggagcg tggggaaaac tccgtgctgt
      301 ttacgatcga tacgatcatg gcggttggta ctcagtccgc gaaacgacct tcggcgttcc
      361 ccgaggaaga aaagatcgac tttcgcaagc tcgcttcggt cgttccacca ccgatggata
      421 gcggagattt cggtgcatcc ttcgagtcac ctccgtcgtt cggagggcct gcgctgggcg
      481 cgcttgccgc cccaacgctt cccacatcca tcgagcccga acccatgcct gagcccgtgg
      541 ccgagcctga agcgatgtat ccttacgatg ctcctgccaa gagcaacaag gggctcatca
      601 ttggattggg tgcggcggtt gtggtgttgc tgctcgtcac ccttggtttg ggtgggtatt
      661 tcctttttgg aagctcggaa gagcccgtgg ctgcgaacac gacgacgccg acggctgagg
      721 ccgtgacggc tccggaagac aaaacggatg ataagagcga gcggacgacc gacaacgaaa
      781 aaaaagatga tggagcgcaa gacgcgaagg ataagaaaga ggacggggcg gacaaggaca
      841 agaaagacga taaaaaagac gaggcaacgg ccaaagcgga tgcgacgggg aaagtagggg
      901 ggcctttacc ggtaggtacg ggcacggcga aagcggttgc cgatccggtc aagagcgatc
      961 ccaaggcaga ttcgaagaaa gacgacgcga agcccgcgga tgttccggcg gcgtccgcgg
     1021 accggaaatt tagccgttcc gcggcagttt ccgcgttgaa tgccgctgcc ggcgcggctt
     1081 cgggatgcaa aaaaccggat ggtcccacgg ggcgcggacg cgtttccgta acgtttgctc
     1141 cttccggaag ggtcaccacg gctgtcgtca atggtccgcc ttttgccggt acttccgtgg
     1201 gtggatgtgt tgcggccgcg ttccgaaggg cgacggttcc tcctttcgat ggaagccctg
     1261 tgacggttca taagtcattt tcgatcaatt gaagctgtat cggccgcttt cggacgatta
     1321 gacgagaggt tgttgttgga cggccgcggc gagtctttgc atcagattgc tgtagcgttg
     1381 ttcccggcgc gtttgggata gggccaattg gatggcacgc ggttcggcga ccagcacgac
     1441 gagtcgctgg ccacgggtaa cggcggtgta caataggttg cgtgacaaca tgatgaaatg
     1501 agcggttacc catggaatga ccacggcggg atactcactg ccttggcttt tatggatgct
     1561 gcatgcgtac gatagtctta gctcatcgag gtggggccga tcgtaaacga tgggtcggcc
     1621 atcgatttcc accacggctt gttcggcgtt tgggtcggta tgagtcacga aaccaaggtc
     1681 cccgttgaac acgtcgcgtg tgtaatcgtt tcgcaactgc atgactttgt cgcccattcg
     1741 caagttcatg ggaaacgggc gctgggggat gtggggggga ttgagcgcgt gttgcaagcg
     1801 ttcattgagg gcttcgatac ccgcgggacc acgacgcatg ggtgtgagca cttgcacgtc
     1861 gcggacggga tcgagatgaa attggcgcgg aatccgctct tggaccaaca aaagcacctt
     1921 gttggcggcg tcgtgcgcat ctttggcagg agcgatgaaa aagtcagaag caaggtcccc
     1981 cttttccggc gtttggggtt ccaatccctc gcgaattcgg tgtgcgttgg caacgattcg
     2041 gctttgggag gcctgacgaa acactttgtg caatcgaacg caagggatgg ctcgtgaatc
     2101 gatgatgtcg cgaagtacgg cccccgctcc gatggatggc aattgatcga catcacccac
     2161 caacacgagg cgagtgcctg tcgcgatggc ccgaatcaat gcgaaactca tggtcaagtc
     2221 cagcatggaa gactcgtcca cgatgatggc tcccgcttcg atgggttttt tttcatggcg
     2281 agtgaatcgt ccgccacgag ggtcgaattc gagaaggcgg tgcaccgtat aagcatcttt
     2341 tccagtggtt tcgctcatgc gacgagacgc tcgtccggtg ggtgcg
//
LOCUS       DAOSOR010000744         2382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_44580_length_2382_cov_4.175333, whole genome shotgun sequence.
ACCESSION   DAOSOR010000744 DAOSOR010000000
VERSION     DAOSOR010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44580_length_2382_cov_4.175333"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..1148)
                     /locus_tag="PK710_24375"
     CDS             complement(18..1148)
                     /locus_tag="PK710_24375"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOT12940.1"
                     /translation="MSICLIPAGSHDPAWLTRAAVEALRHADVVYCESGAWFSDIVAR
                     DARRIEVSACRSACLRMMDDARAGQRVVRVYDRVGWKDRIVLRDMRMLRDASLAFELI
                     PSTEPQADRWANWVEQRPLLGRRVVVLRMVGQASTTAELLVERGADPWVVPTIELHPP
                     EDVEAFRTALRNLGGYDLVAFTSTNGVDKTWEQLRELGLDARAFGACRIAAIGDATAR
                     QLLNHGIVADVKAKEFRGEGLADGIIEQMGNLRGKRILIARAMEAREVLPEALQAAGA
                     DVDVVAAYRTLPAPPETMEPLREGLRHNEVDVVLLTSSSTVTNLCSALGQEAAMLLSR
                     ACLASIGPITTAQAKKLGLTVGVEAATFTVPGVVQALESYFR"
     gene            1348..1803
                     /locus_tag="PK710_24380"
     CDS             1348..1803
                     /locus_tag="PK710_24380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12941.1"
                     /translation="MARTQVVLIAIILSTMAGCTKPQATNHSTQPSEAASHPVVGGRC
                     DYQSTAGHVVVERIESAPETENNCATDPVRVVLRFTPSDGASEPTTDTLTIFDGKNPP
                     RSCLAAAGIMEGAKFPATHKQITSGTCTPSLLEPNIPALDSCRDACFTK"
     gene            1884..2228
                     /locus_tag="PK710_24385"
     CDS             1884..2228
                     /locus_tag="PK710_24385"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HOT12942.1"
                     /translation="MTYTASLPVTESFSRGSKRGMLARIPFWADVGAFWRYFRDRNAS
                     PWGKIFALLTVAYVVMPVDAIPDIAPVLGWLDDLGLMLVALTYLASVVRKYRNDSAPR
                     IDDDKPIEPSAL"
BASE COUNT          527 a          805 c          584 g          466 t
ORIGIN      
        1 caatctggca atgagaatca tcgaaaatag gactcgaggg cttgtacgac acccggaacg
       61 gtaaacgttg cggcctccac gccgaccgtc aaacccagtt ttttcgcctg cgccgtggtg
      121 atgggcccaa tactcgccaa acacgctcga gacaatagca tcgctgcctc ctgccccagc
      181 gccgaacaca gattcgttac cgtcgaactc gacgtaagca acaccacatc cacctcgttg
      241 tgcctgagcc cttctcgcag aggctccatc gtttccggtg gggctggcaa tgtgcggtac
      301 gccgcgacga catcgacatc ggctccggcc gcttgcaatg cctcgggcaa tacctcccgc
      361 gcctccatcg ctcgcgcgat gaggattcgc ttgccgcgaa ggtttcccat ctgctcgatg
      421 atgccatcgg ccaacccttc ccctcggaac tccttggctt tcacgtccgc aacgatgcca
      481 tggttcaaca actgccgggc tgttgcatca ccaatcgccg cgattcgaca agccccgaac
      541 gcacgcgcat ccaatcccaa ttcgcgaagc tgctcccatg tcttgtcgac cccgttggtg
      601 ctggtaaacg ctacgagatc atagccgcca aggttccgca acgccgttcg aaacgcttcg
      661 acatcttctg gaggatgcag ttcgatcgtg ggaaccaccc atggatccgc cccccgttcc
      721 acaagcaatt ccgccgtcgt actcgcttgc cccaccatgc gtaacaccac cacccgtcgc
      781 cccaataacg gccgttgttc cacccagttc gcccagcgat cggcttgcgg ttccgtggat
      841 ggaatcaact cgaacgcaag cgaagcgtca cgcaacatgc gcatgtcccg aaggacaatc
      901 ctgtctttcc atcccactcg atcataaacc cgaaccacac gctgtcccgc gcgtgcatcg
      961 tccatcatcc gcaaacacgc cgaacggcaa gcagatacct cgatgcgcct tgcatcgcga
     1021 gccacgatat ccgaaaacca tgcgccgctt tcgcaataca cgacatcggc gtggcgcaat
     1081 gcctccaccg cggctcgagt caaccaggcc ggatcgtggc ttcccgcagg aatgagacaa
     1141 atggacacca ccaggccaaa ctagccccga cccgttgcaa agggaaagac caaaggcatg
     1201 agcccccatc gatgagccgc ccgctgtccc caggccatgg attcccccat cgaaaaggtc
     1261 gatccgagtc gaagaaggag gccttcggaa aagccattgc ccgatagctc gaccgaatcg
     1321 tacgctatcc atgtccgagg tatcaccatg gcgcgtacac aagtcgtttt gatcgcgatt
     1381 atcctttcca cgatggcagg atgcaccaaa ccacaagcca ccaaccattc cacgcaaccc
     1441 tccgaggccg cctcccatcc ggtcgtcgga ggccgctgtg actaccaatc cacggcagga
     1501 cacgtcgtcg tggagcgaat cgaaagcgct cccgaaacgg aaaacaattg cgcgaccgat
     1561 ccggtacgcg tggtgttgcg ctttactcct tccgacggcg catccgaacc aacgaccgat
     1621 acccttacga ttttcgatgg gaagaaccct ccgcgttcgt gcctggctgc cgccgggatc
     1681 atggaaggag caaagtttcc cgctacccac aaacaaatca cctccggtac atgcacccct
     1741 tccttgctcg aaccgaatat ccctgccctg gactcctgcc gcgacgcttg cttcaccaag
     1801 tgaatccatg acgcatcgcg tggttgccct gccgaaatac agccttacat tgatcggcac
     1861 tctcaaccgt cgaacggagt gccatgacct acacagcttc tcttcccgtc acggaatcgt
     1921 tttcccgcgg ctcgaaacga ggcatgctgg ctcgtatccc tttttgggca gatgtcggcg
     1981 cgttttggcg gtacttccga gaccgtaacg cctcgccgtg gggaaagata ttcgccctgc
     2041 ttaccgtcgc ctatgtcgtc atgcccgtcg atgccatccc cgatatcgca cccgtgttgg
     2101 gatggctcga tgacctcggc ctgatgctcg tagccctgac gtacctggcc tcagtcgtgc
     2161 gaaaatacag aaacgacagc gctccacgta tcgacgatga caaacccatc gaaccgtctg
     2221 ccctgtgata ccgatgatca agcggggata tcgtcacaca cgcggaatcg atcaggtggg
     2281 attgcgagta cacaccacat catcgacctg aatatggtcc ttcgaggcat cggtgtaact
     2341 cgcgtacaga atgatgcgca tgtcgtgaac gttgtcgttg at
//
LOCUS       DAOSOR010000745         2380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_44636_length_2380_cov_5.499355, whole genome shotgun sequence.
ACCESSION   DAOSOR010000745 DAOSOR010000000
VERSION     DAOSOR010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44636_length_2380_cov_5.499355"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..149
                     /gene="infA"
                     /locus_tag="PK710_24390"
     CDS             <1..149
                     /gene="infA"
                     /locus_tag="PK710_24390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOT12943.1"
                     /translation="GSENTMKLTATLCGKMRSRYIKVTTGDRVKVELSPYDLTRGRIV
                     YRMR"
     gene            complement(279..923)
                     /locus_tag="PK710_24395"
     CDS             complement(279..923)
                     /locus_tag="PK710_24395"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOT12944.1"
                     /translation="MTQASSPTNPPQGRIIAVAGNIGVGKSSLVEWLRQHFDVVPFFE
                     PNEDNPYLPNFYQDMPRWAFQSQVFFLIRRFRLHRAMENESHDVVQDRTIYEDAEVFA
                     THLHRSGIMDERDWQVYTELYHTLRRELRPPDLLVYLRCPVEVLRKRIRQRGRAFEQS
                     VARPYLKALDKLYAEWFERYDLSPTLVIDTHHLDYVQNLFDRQELVERVRTLLA"
     gene            complement(920..1567)
                     /locus_tag="PK710_24400"
     CDS             complement(920..1567)
                     /locus_tag="PK710_24400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12945.1"
                     /translation="MSNAEPHAQADLAPRAEASILAGLTNRYAQSNLDGIAYEPALQL
                     SFQARGYVWSWLNAAVYHRRASHGIDLPRGAAGFDYERIEIDKALAYSLGARIEPTYR
                     VSDVFRAWVSVGLGWGRMSLDRVHVQEATRSYTVRERAGVFVEVPMGIGASYELVPRW
                     LTVQMEADIAPLSKQSGNLFGPTPYVDSNGSLHFTGSFPTQSLSTSFLLGVSFLL"
     gene            complement(1694..>2380)
                     /locus_tag="PK710_24405"
     CDS             complement(1694..>2380)
                     /locus_tag="PK710_24405"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HOT12946.1"
                     /translation="CANVPIQVPTCDHIEETNLPLEMFGRRYIVTDLFNPAGRPRQMV
                     RIHGLVDDTLVSFDPPSAYQSIRVRRGETVDLDLPKEQSSAREFMVSSTHPIGVTQYM
                     VGNLAEPYQAQAAGINLGDPSQTFVVPVSRYLKRYFLAVPTGFEQHRVDIVASTGSEV
                     YFDGRLISPESFRAVGASGFSVARLEHLEPGKRHEIRGEKALGVQVYGFGPYTSWMAP
                     GGIELRSALP"
BASE COUNT          588 a          710 c          601 g          481 t
ORIGIN      
        1 atggttccga aaataccatg aaactcaccg caactctttg cggaaaaatg aggtcacggt
       61 acatcaaggt caccacagga gaccgcgtca aagtcgaact ttccccctat gacctgactc
      121 gcggacgcat cgtgtatcga atgcgttgac ctttcaccca ttcgcccgtg ggataatcct
      181 tcgcatcgtt tcagtttgct tccgtgtccc gccagcgccg ggctgccgca gtcagccccc
      241 gctagccgcg atcgtcaccg agtcgacgat gacaccgctc aagcaagcaa tgtgcgaacc
      301 ctttccacga gttcttggcg atcaaataaa ttctgcacat agtcgaggtg atgcgtgtcg
      361 atgaccaagg ttggcgaaag atcataacgc tcgaaccatt ccgcatagag cttgtcgagc
      421 gccttgagat agggacgagc cacgctttgc tcaaatgcgc gaccacgctg ccgaatacgt
      481 tttctcaaca cctccacggg acatcgcaag tagaccaata aatcaggcgg cctgagttcc
      541 cgccgcaacg tatgatacag ttccgtgtac acctgccagt cacgctcatc catgatgccg
      601 cttcggtgca agtgcgtcgc gaatacctcc gcatcctcgt agatggtccg gtcttggacg
      661 acatcgtggg attcattttc catggctcga tgcagacgaa agcggcgaat aaggaaaaat
      721 acttgtgatt gaaaagccca tcgaggcatg tcctgataaa aattcggaag ataagggttg
      781 tcttcgttgg gctcgaaaaa tggcacgaca tcgaaatgct gccgtaacca ttcgaccaag
      841 ctcgacttgc ccacccctat gttgccggcg acggcaatga tgcgaccttg ggggggattg
      901 gtgggggaac tcgcctgggt cacaacagaa acgatacgcc caataagaag ctcgtggaaa
      961 gactctgggt cggaaaggag cctgtaaaat gcaacgaccc gttggaatcc acataaggcg
     1021 tcggcccgaa tagattcccg gattgtttgg ataatggcgc aatatccgct tccatctgga
     1081 ccgtgagcca gcgaggtacc aattcgtacg aggctccaat tcccatcgga acctcgacga
     1141 aaacacccgc gcgctcacgc acggtgtacg atcgggtagc ctcctgcacg tggacgcggt
     1201 ccaaactcat tcgaccccat ccaagcccca cgctcaccca ggcccgaaag acgtccgata
     1261 cgcggtacgt tggctcgatt cgtgcgccca aactataggc gagcgccttg tcgatctcga
     1321 tccgctcata atcgaatcct gcggcgcccc gtggcaagtc gatcccatgg gatgctcggc
     1381 gatgatacac cgccgcgttg agccatgacc aaacatatcc tcgcgcttga aaggaaagct
     1441 gaagagctgg ttcgtaggca atgccatcga ggttggattg ggcataccga ttcgtcagtc
     1501 ccgcaagaat cgatgcttcc gcgcggggag ccaaatccgc ttgcgcgtgt ggctcggcgt
     1561 ttgacatcgc gctcgatagc cagacgcagg gcacgagcgc caatcgacat cggcgtatga
     1621 aaaacgaagg agtcacgaaa accatcgcag cctaatccat gctttgcgca atgcgtagcg
     1681 gctttgtgca aagctatggc aatgccgagc gaagctcaat tcccccaggt gccatccaac
     1741 tcgtatacgg cccaaacccg tacacttgaa cgcccaacgc tttttctccg cgaatctcat
     1801 ggcgtttgcc tggctcgaga tgctcgagtc tcgctacgct gaacccgctc gctcccaccg
     1861 ctcgaaacga ttcgggcgaa atcaaacgac catcgaaata cacctccgat cccgtcgaag
     1921 ccacgatatc cacacggtgc tgctcaaacc ccgtcggcac cgctaggaag tatcgcttca
     1981 aatatctgga caccggaacc acgaacgttt gactcggatc cccaaggttt atcccagcgg
     2041 cctgcgcctg ataaggctcc gcaagatttc cgaccatgta ttgcgtcacc ccaatcggat
     2101 gggttgacga aaccatgaac tccctcgccg atgattgttc tttcggtaga tccaaatcca
     2161 ccgtctcgcc acggcgaaca cgaatcgatt gataagccga tggcggatcg aatgaaacca
     2221 aagtgtcatc caccaaacca tgaatacgca ccatctgacg tggacgcccc gccggattga
     2281 atagatcggt cacgatgtag cgacgaccaa acatctcaag cggcaaattg gtctcctcga
     2341 tatgatcaca ggtcggcacc tgaatcggaa cattggcaca
//
LOCUS       DAOSOR010000746         2379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_44673_length_2379_cov_3.544750, whole genome shotgun sequence.
ACCESSION   DAOSOR010000746 DAOSOR010000000
VERSION     DAOSOR010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44673_length_2379_cov_3.544750"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..1330
                     /locus_tag="PK710_24410"
     CDS             272..1330
                     /locus_tag="PK710_24410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOT12947.1"
                     /translation="MTSPYATPSFVQQLLSKAIAAGASDVHLKVGQPPGARVRGRMVY
                     FRMDNVVPDDTEAAATTILQEYRGAPSIKDITEVDTSYAVPGVGRFRVNVYRQRGSLA
                     IVMRTIPLDIPTFEQLGCPEAVKLLADKERGMVIIVGAAGNGKSSTLAAMVGYLNRTR
                     PIHVITIEDPIEFLHKDEMASISQREVGIDTPSFAIALRAALRQDPDVILVGEIRDQE
                     TMDIALKAAETGHLVLTTLHTPDVTRTINRMIALFDLADPTELRDRIANSLQGIVAQR
                     LLPKVDGEGLVLASEVLVMTGTARETIRRPENNPPLKEVMEKGVHPYGMQTFPMDLKR
                     LMQLGVISKDVARAAAGF"
     gene            1477..>2379
                     /locus_tag="PK710_24415"
     CDS             1477..>2379
                     /locus_tag="PK710_24415"
                     /inference="COORDINATES: protein motif:HMM:NF023649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HOT12948.1"
                     /translation="MRFYLWIPWLAWSLVGCGSESETDAATHEAGIDAHADAQVDAAV
                     DAREDAVEDAVTDSSIDGAETGDSSDVAPDIEKDTQNVDATNDVLEAGLPTGCLVGDY
                     QPYFGNLHAHTSYSDGKGLPSEAFAHARQQAGLDVQVITDHLEQLYWPNPSGRWDKCR
                     QQADDAYEPGAFVALCGFEYGSGFHGIKSTGHNNVFFSDKLFPMVQLDFRDFYKTLVA
                     CANCIGQFNHPGDESEQTWSGFEYHADADDRIQFFEFNGAGDVWNLYFAALDKGWHVS
                     AVMNQDNHSADWGTKNDNRSGLWMP"
BASE COUNT          539 a          682 c          654 g          504 t
ORIGIN      
        1 aaacggattc cgccgctcaa cggagaaaag cgcggcttcg aagaaaaatc gcccgcgcca
       61 actctagcag caacgccacc gaggaacaag ccagcctccc ccttgctctc cacaccacaa
      121 aaaacgttga tattacaaca ggttaggagt gtgtggcaag accgtttcgc aagaattttt
      181 ccgcgtcagc ccttcggaaa gcctgccatg aagcatcgag gcatgggatc ctgttgccaa
      241 acaacccagt tttcacctat ggtttcatgt cgtgaccagc ccctacgcta ccccttcttt
      301 cgtccaacaa ctcctgtcca aagccatagc cgcgggagcg agtgacgtcc atctcaaagt
      361 cggacaaccc ccaggcgctc gcgttcgtgg acgcatggtc tattttcgga tggataatgt
      421 tgtccccgac gacacggagg ccgcggccac gaccatattg caggagtatc gaggcgcgcc
      481 cagcatcaag gacatcacgg aggtcgacac ttcttatgcc gtccctggcg tgggacgttt
      541 tcgcgtcaat gtataccgtc agcgcggaag ccttgcgatc gtgatgcgga ccattcccct
      601 ggatattccc accttcgaac agcttggctg ccccgaggct gtgaaactac tcgccgacaa
      661 agagcgcggg atggtcatca tcgtgggagc cgccggcaac ggaaaatcct ccacgcttgc
      721 ggccatggtc gggtacctca accgtacgcg tcccatccac gtcatcacca tcgaggatcc
      781 catcgaattc ctccacaagg atgagatggc ctccatcagc caaagggaag tcggtatcga
      841 taccccgagc tttgcgattg cgttgcgggc cgccctccga caagatccgg acgtcatttt
      901 ggtcggtgag atccgagacc aggagaccat ggatatcgcg ttgaaggcgg ctgaaaccgg
      961 gcaccttgtc ctaaccacgc tccacacccc ggacgttacc cgaaccatca accgcatgat
     1021 cgcgcttttt gacctggcgg accccaccga actgcgcgat cgcattgcca acagtctgca
     1081 aggcattgtt gcgcaacgtt tgctgcccaa ggtcgatggg gaggggctcg tactcgcctc
     1141 tgaggtgctg gtcatgacgg gtaccgcacg cgaaaccatc cggcgccctg aaaacaaccc
     1201 accgctcaaa gaggtcatgg aaaagggggt tcacccttat ggcatgcaga ccttccccat
     1261 ggacttgaag cgactcatgc agctcggggt gatttcgaaa gacgtggccc gcgctgcagc
     1321 ggggttttga agccgccatc cccctcactc ataaaatagc tataaattca attggttaca
     1381 aatgctgccc cgggaccatt gtcaccgatt tcgattttgc ttttccctgt caaccaaagg
     1441 tgggaaaaaa attgaaacct gctatcttcc ctggtgatgc gtttctatct ctggatacct
     1501 tggttggcct ggagcctcgt ggggtgcgga tccgagtcgg aaacagacgc cgctacccac
     1561 gaagcaggga ttgatgccca cgcggatgcc caagtcgatg ctgccgtcga tgcgcgggag
     1621 gatgccgtcg aggatgcggt caccgattcg tccatcgatg gggcagagac gggagattca
     1681 tctgatgttg cgcctgatat cgaaaaggat acacaaaacg ttgacgctac aaatgatgtt
     1741 ctcgaagcgg gtttgccgac gggatgtctg gtaggtgatt accaacctta tttcgggaat
     1801 cttcatgcgc atacgagcta ttccgatggc aaagggcttc cttcggaggc gttcgcccac
     1861 gcgcgccagc aagcggggct cgatgtccag gtgatcaccg accacctgga acagctttat
     1921 tggccgaacc cttcgggacg ctgggacaag tgtcggcagc aagcggatga cgcctacgag
     1981 cccggtgctt tcgtggcctt atgcggtttc gaatatggtt cgggcttcca cggcatcaaa
     2041 tccacggggc ataacaacgt attcttttcc gataagctat ttcccatggt tcagctcgac
     2101 ttccgagact tttataaaac actcgtggct tgcgcgaact gtatcgggca attcaatcat
     2161 ccgggcgatg agtccgagca aacgtggtcg gggttcgagt atcacgcgga tgccgacgat
     2221 cgcatccagt ttttcgagtt caacggcgcg ggcgatgtgt ggaatctgta cttcgccgcg
     2281 ttggataaag ggtggcatgt atccgcggtg atgaaccagg ataaccatag cgcggattgg
     2341 ggaacgaaaa acgacaatcg aagcggattg tggatgcct
//
LOCUS       DAOSOR010000747         2377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_44714_length_2377_cov_4.639104, whole genome shotgun sequence.
ACCESSION   DAOSOR010000747 DAOSOR010000000
VERSION     DAOSOR010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44714_length_2377_cov_4.639104"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..711
                     /locus_tag="PK710_24420"
     CDS             199..711
                     /locus_tag="PK710_24420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12949.1"
                     /translation="MRRFWRPWVFVAQTVLMVGLGRIGLGCGTDPVNPEGCKDIEFAR
                     CEAAPACPAFSPDFDVTGCKRFYRDQCLKGLQTASDPGNPRIDACTRAIQKASECAHN
                     QIAPCSIGSREIPDPCALITNPEDFTECGFLDDYYGEDSGGQPPPQDASTEPPVQQEA
                     GTDTDAAAKE"
     gene            737..1279
                     /locus_tag="PK710_24425"
     CDS             737..1279
                     /locus_tag="PK710_24425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12950.1"
                     /translation="MPPFSLRFACTLLFLASFLFGCGGKGEPPLPPSKPFGPDERILV
                     VAYSWRESTQSEGYLHEPDVAYMRVKDTFTLHLEEKSLTALVDVNRVELLRNPEGREF
                     HCKVTGAIKASVTYAWLNGEPTVAVNVPPASLPRRCKESGFSRLSKQFPALSATYALR
                     GDQLIAIEPITLRSALLPAN"
     gene            complement(1380..>2377)
                     /gene="cls"
                     /locus_tag="PK710_24430"
     CDS             complement(1380..>2377)
                     /gene="cls"
                     /locus_tag="PK710_24430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOT12951.1"
                     /translation="YLIRPDITGRWFLYKLIAAANRGVDVRLLCDGWGCFALPRGWIK
                     QLRMQGVQVAFFFPVSSMLMQPVSLRNHRKIVVVDATIAFTGGINIGDEYLGKMESVG
                     AWRDTHIRIQGPAAASLQSVFLRDWAFSTQERPERESLFPASNEAVGEAHASILTSGP
                     DTDSEAIHRVFFGAIAGAKERVYITTPYFVPDRALIVALQMAALQGVEVRIIVPSRSN
                     HSLTLHAGRSYYEELIEAGVHIHEYSPGMIHAKMMVVDGCIALVGSANMDLRSFRLNF
                     EVHALIGDEYTSRALERSFFIDLAQTKEIDLQAWRKRRWHSRVAEGAARLVSPLL"
BASE COUNT          502 a          693 c          646 g          536 t
ORIGIN      
        1 ccgcaaaaga cgcggctctg ggcggcgacc atcactcgtt gggttgtgcc gatatccttg
       61 gggctcgcat tgctgaggtt ggtggtttat tttttgaagc cgtgacttcg gggggatggc
      121 gtgatggcag ggggcaaggc gtttttgcat tgtttgcaaa cttggccgaa tcgcaggacc
      181 gtgctacgcg cccagttcat gaggcgcttt tggcgaccct gggttttcgt tgcccaaacg
      241 gttttgatgg ttggactggg aaggattggg ctcggttgtg gcaccgatcc ggtcaacccg
      301 gaggggtgca aggacattga gtttgcgcgt tgcgaagctg ctcctgcatg cccggctttc
      361 agcccggact tcgatgtcac cggctgcaag cgattttatc gggatcaatg tttgaaggga
      421 ctacaaaccg ccagcgatcc tggcaacccc cgcatcgacg cttgcacgag agccatccaa
      481 aaagccagcg aatgcgcgca caaccagatc gctccttgct ccatcggctc gcgagaaatc
      541 ccggatccct gtgctttgat caccaatccg gaagatttta ccgaatgcgg ttttctcgat
      601 gattattacg gcgaggattc tggtggtcag ccaccgcccc aagacgccag cacggagccg
      661 cccgtccaac aagaagcggg taccgacacg gatgcagcag ccaaggagta ggaataggca
      721 gaaaaatcgg tgccccatgc cccccttttc tctccgattc gcctgcacac tgctatttct
      781 cgccagtttt ctattcggtt gcggtggcaa aggcgagcct ccccttcccc cttccaagcc
      841 attcggccct gacgaacgaa tcctcgtggt cgcgtattct tggcgtgaat ccacgcaatc
      901 ggaaggttac ctccacgaac cggacgtcgc ctatatgcgc gtcaaagaca cgtttacgct
      961 gcatctcgaa gaaaaatccc tcaccgcgct cgtcgatgtg aatcgcgtcg agttgcttcg
     1021 caatcccgag ggaagggagt ttcactgcaa agtcacgggc gccatcaaag cttccgtgac
     1081 gtatgcgtgg ctgaacggcg agcccaccgt agcggtcaac gtccctcccg cttccctgcc
     1141 ccgtcgatgc aaagaaagcg gtttttcgcg tctttcaaaa caatttcccg cactgtccgc
     1201 cacctatgcg cttcgggggg atcaactcat tgccattgag cccatcacgt tgcgaagcgc
     1261 gctattgccg gccaattagg cgtattggcc ctgcgattac ggtcgagggc gggtgcgagg
     1321 cagtgaaaac tcgctgccga ttgcgtgctc tctatcccga tttccattcc aatttcggct
     1381 cataataacg gggaaaccag cctggccgcg ccttccgcca cgcgagagtg ccatctgcgc
     1441 ttgcgccacg cttgcagatc gatctctttg gtttgtgcca aatcaatgaa aaatgaacgt
     1501 tccagagcgc gtgacgtata ctcatcgccg atgagggcat gcacttcgaa gttgagtcga
     1561 aaacttctca gatccatgtt tgcgcttccc accagggcaa tgcatccatc cacgaccatc
     1621 attttcgcgt gaatcatgcc gggggagtat tcatggatgt gaacgcccgc ctctatcaat
     1681 tcttcgtaat agctcctgcc ggcatggagc gtgagcgaat gattgctgcg cgatggcacg
     1741 atgatccgca cttccactcc ttgcaaagcg gccatttgca gcgcaacgat gagcgctcga
     1801 tcgggaacga agtagggtgt cgtgatgtag actcgttctt tggctccggc aatcgcacca
     1861 aaaaacacgc gatggatggc ctcactatcc gtatcgggac cgctggtgag aatcgacgca
     1921 tgggcttctc cgaccgcttc gttggacgcg gggaagagcg attcgcgctc gggtcgctct
     1981 tgggtcgaaa aggcccaatc tcttagaaac acgctctgca gcgaggctgc cgcgggacct
     2041 tggatccgaa tatgagtgtc gcgccacgcc cctaccgact ccatttttcc caggtattca
     2101 tcgccgatat tgattcctcc ggtgaacgca attgtggcat cgaccaccac gattttcctg
     2161 tgatttcgca gggataccgg ttgcatgagc atcgaggata cgggaaagaa aaaggctact
     2221 tgcacccctt gcattcggag ctgtttgatc catccccgcg gcagcgcaaa acatccccag
     2281 ccatcgcata acaatcggac gtcaacgcca cgattcgccg cggcgatgag tttatacaag
     2341 aaccatcgcc ccgttatatc cggccgaatc aagtaat
//
LOCUS       DAOSOR010000748         2377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_44716_length_2377_cov_4.236434, whole genome shotgun sequence.
ACCESSION   DAOSOR010000748 DAOSOR010000000
VERSION     DAOSOR010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44716_length_2377_cov_4.236434"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2125
                     /locus_tag="PK710_24435"
     CDS             <1..2125
                     /locus_tag="PK710_24435"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT12952.1"
                     /translation="VDYNVIWRLFLSRLRNLRKMGDGSLRIRYLDPGDGEWKHESFDM
                     VVLSTGQEKPVSPQRLAAMIGLDAPPEGLDTPQGFEKVRSSKPGIFLCGSLLGLTDIS
                     EALTAGSAAAGEASKLLHQLGKPYEVEVALPPERPVARERPRVHVLLCRCGDNRASQA
                     VDFALSAQRLRAIPGVGQVDVVDTLCRGDGFASSEQLLRHSKCNRVVFAACMPYIYRR
                     RLRAIAEKAGFNPALVEVVDWLSLLPSTSAEGQEASLMRQREMRVMVGIEKLKATDPV
                     PTVEVAVYPRVLVVGGGVAGMRAALSLAQRGIGVDLVESRGELGGRVRQDLHYTLDGL
                     DPRGLVEGLMQAVRENPRIQVHTETRLSRTQGTVGRFRSVLEQGEKRITIEHGATIVA
                     TGGKEAGTTLYGYGSSERVMTQSEVEKGLSEGSLDAAALDTVVMIQCVGSREPGAHNY
                     CSRVCCAAALKNANQLRKANPQVRIVILYRDMMTYGFLESYYTEARRQGVLFASYDPA
                     DKPRVQFENGKPVVTFTDTILRDEVRVSADLLCLSTGVEPDPSHQELGEKLGIALTQD
                     GFFQEAESKWRPVDCLKEGVFLAGLAHSPRPIVEVLAQAEAAAQRAFVYLSKGRLTAA
                     RMTSKVHDSLCARCRTCITACPFEARSYDVVNHRVTVDAATCQGCGICAVACPNGAAE
                     LKGPNDRQNLAIIEASLMGSWSAGP"
     gene            2177..>2377
                     /locus_tag="PK710_24440"
     CDS             2177..>2377
                     /locus_tag="PK710_24440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12953.1"
                     /translation="MDWSVELSSSVDKEELKWVRETLEAKLKAESGQYGEAARILWEL
                     LVQIRHTKNSRWECLTMIHMGKV"
BASE COUNT          479 a          528 c          844 g          526 t
ORIGIN      
        1 tgtcgattac aacgtaatct ggcgcttgtt cctctcccgt ttgcgaaatc tacgtaaaat
       61 gggtgatggg tccttgcgta tccgctatct cgacccgggc gatggcgaat ggaagcacga
      121 aagcttcgac atggtggtgc tgtcgacggg tcaagaaaag cctgtgagcc cccagcgctt
      181 ggcggctatg ataggtttgg atgcaccgcc ggagggactt gacacaccgc agggattcga
      241 aaaggtgcgt tcctcgaagc ctgggatatt cctttgcggg tctttgctgg ggctgaccga
      301 catcagtgag gcattgacgg cggggagcgc ggcggcagga gaagcctcca agctcttgca
      361 tcaactcgga aagccgtatg aagtggaggt ggcgctgccg ccggaacggc ccgtggcaag
      421 ggagcgtcct cgcgttcatg tcctattgtg tcgctgtggg gataaccgag cttcccaggc
      481 cgtggacttt gcgctgtctg cccaacggtt gcgcgcgatt ccgggggttg ggcaggttga
      541 tgtggtggat acgttgtgcc gaggagatgg gttcgcgtcg tcggagcaac tccttcgcca
      601 ttcgaagtgt aaccgagtcg tcttcgccgc gtgcatgccc tatatatatc gtcgccgcct
      661 gcgggccata gcggaaaaag ccggattcaa tccggcactc gtggaggttg tcgattggtt
      721 gagccttctg ccttcgacaa gtgcggaagg gcaggaagct tctttgatgc ggcaacgtga
      781 gatgcgagtg atggttggga tcgagaaact caaggcgacg gatcctgttc cgacggtcga
      841 agttgctgtt tatccgaggg tgcttgtggt ggggggggga gtagcgggga tgcgagccgc
      901 tttatctctt gcgcaacgtg gcattggggt ggatttggtg gagagtcggg gggagcttgg
      961 gggaagggtg cggcaggatt tgcattatac gttggacggg ttggatcctc gggggttggt
     1021 cgaggggttg atgcaggcgg tccgggaaaa cccgcggatt caggtgcata cggagactcg
     1081 gctttccagg acgcaaggga cggtggggcg ttttcgaagc gtgttggagc aaggggaaaa
     1141 gcggataacg atcgagcatg gtgccaccat tgtggccacc ggggggaaag aggcgggaac
     1201 cacgttatat gggtatggtt ccagcgagcg cgtcatgacg caaagcgagg tggaaaaggg
     1261 gctttccgag gggagtctcg atgccgcggc gttggatacg gtggtgatga ttcaatgcgt
     1321 gggttcccgc gagccagggg ctcacaatta ttgcagccgg gtatgttgtg cggcggcgtt
     1381 gaaaaatgcg aatcaactga gaaaagccaa tccgcaagtg cgaatcgtca tcttgtaccg
     1441 ggatatgatg acctacgggt ttctcgaatc gtattatacg gaagccaggc gccaaggggt
     1501 gctatttgct tcttacgacc ctgcggacaa gcctagggta caattcgaga atggaaagcc
     1561 ggtggtgacg tttacggata ccattcttcg cgacgaggtt cgggtgagtg cggatttgtt
     1621 gtgtttgagt acgggggtgg agcctgatcc gagccatcaa gaattaggcg aaaaactggg
     1681 tattgcgctg acgcaagacg ggtttttcca ggaagcagag tcgaagtggc gtccggtgga
     1741 ttgtctgaag gaaggggtat tccttgcggg actggcgcat tctccgcggc cgatcgtgga
     1801 ggttttggct caggcggaag cggcggcgca gcgagcgttc gtatatcttt cgaaggggcg
     1861 tttgacggct gctcggatga cgtcgaaggt tcacgacagt ctgtgtgcgc gatgcagaac
     1921 ctgtattacg gcctgtcctt tcgaggcacg ctcctacgat gtggtgaacc atcgtgtgac
     1981 ggtggatgcg gcaacgtgtc aagggtgtgg gatttgcgcg gtggcctgtc cgaatggggc
     2041 cgcggagctg aaagggccga atgataggca gaacctggcg atcatcgagg catccttgat
     2101 ggggtcttgg tctgcgggac cgtaggcaaa tccacgcttc ccggactggg cttcagccca
     2161 gtggatcgga gggaccatgg attggtcggt ggagttgagt tcttccgtgg acaaggaaga
     2221 actgaaatgg gttcgggaaa cccttgaagc gaagctgaaa gccgaatccg gtcaatacgg
     2281 ggaggcggct cggattctgt gggagctgtt ggtccagatc cgacatacga aaaacagtcg
     2341 atgggaatgc cttaccatga tccacatggg caaggta
//
LOCUS       DAOSOR010000749         2369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_44929_length_2369_cov_3.461971, whole genome shotgun sequence.
ACCESSION   DAOSOR010000749 DAOSOR010000000
VERSION     DAOSOR010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44929_length_2369_cov_3.461971"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PK710_24445"
     CDS             <1..192
                     /locus_tag="PK710_24445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12954.1"
                     /translation="SQVLDDACDAVQAVYACLSKRSANRVLPTTRPHTHDTPTAKPSS
                     TSASQSPIHEAVLPLPCHP"
     gene            complement(358..501)
                     /locus_tag="PK710_24450"
     CDS             complement(358..501)
                     /locus_tag="PK710_24450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12955.1"
                     /translation="MRDGDSSQDALLLGWDGRPRIVGCALLGKEALSRDATDCRHISI
                     RGA"
     gene            complement(835..2127)
                     /locus_tag="PK710_24455"
     CDS             complement(835..2127)
                     /locus_tag="PK710_24455"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT12956.1"
                     /translation="MVEPVCRLLGYFCAIRWGGLQGEPVCKVSPSADGSRAQQSDAAN
                     ARVGDGCVWYVRLLLVVLSMSVWMGCGAHGSAAPRGDSVDRHTEVEPKTDFAVKVLEG
                     RGIRYCGGGFEYEGEIFFEYNKDTLKRDREETDATIGKWVEYLKRYPKLRVKVVGHTD
                     SRGASLMNANLSLRRARSVARELRNRGVPAGQLDEPEGMGEEGTEQIEGPDCYNKEPE
                     GCEDKETPGDRCKCKAVWAKSRRVEFAPVEGTDALEEDCGQAAVGEAAKAEPDPESAC
                     PENLWGIHINAGRPNHFVGAALAWQPLCWLEVTGGVGFWPKKSVEGDGESGKYSAWSI
                     PIRARFWPGRHHSFIADVGGGYIQVNVDVREPIRNESDSTSYGRNFGFLGIGYGYRSN
                     GAFRLALLGGVMAMGKIGRFESISEPTVYPELSVGFLW"
BASE COUNT          539 a          809 c          538 g          483 t
ORIGIN      
        1 agccaggtgc tcgacgacgc ttgcgacgct gtgcaagcag tatatgcttg tctgagtaag
       61 cgctccgcca accgcgttct tcccaccacg aggcctcata cccacgatac gcctacggca
      121 aagccttcca gcacatcagc ttcccaatcc cctatccacg aagcggttct accgctgccc
      181 tgccacccct gagccatctc ctcatttttc ccgaagcctt tccttcgccc tacgactact
      241 ggaaacaagt acctatcatt gcgtggccgc agtcacggca acggtcacgc atacggtcac
      301 ggtccacgac acacaaaacc ggtcacggta cagcaaataa gtccacaagg cgggatacta
      361 cgctccccgt atcgaaatat gccgacaatc cgtggcatcc cggctcaacg cttccttacc
      421 aagcaaggcg catccgacga tccgcggacg accatcccag ccgagcagca gcgcgtcctg
      481 gctggaatca ccatcacgca cctcgcacgg aatgagaagg cttggtagcc cacgagcatc
      541 accacacagc gctcaactga ccgtatttat tgatgtttct acaaggctga tcccaatggc
      601 cccgagggcc aggtcgaagt caaaatccag gggattccct gctcgtattc ctttccaagg
      661 caagaaaatg agcggatctg tcagcctgcg ctggagttac cgcaactcga aggagggcgg
      721 caccagtcga atggatgggt ggaagaaagg gagccgcgta tctgcaatat ccccacccag
      781 gttgccaagg gtagaatggg atacgttgtt taccaaaaag caaggaagct cccgtcacca
      841 caaaaatccg acggacagtt ccgggtacac cgtcggctcc gaaatgctct cgaatcggcc
      901 gatcttgccc atcgccatga ctcccccgag aagcgcaaga cggaaagctc cgtttgaccg
      961 atagccgtag ccgataccaa gaaatccgaa atttcgacca tagctcgtgg aatcgctctc
     1021 attacgaatc ggctcacgga cgtcgacgtt cacctgaatg tatccgcctc ccacatctgc
     1081 gatgaaagag tggtggcggc ccggccagaa ccttgcacga ataggaatgc tccaagccga
     1141 atacttcccg ctctccccat ctccttccac actctttttc ggccagaaac cgactccccc
     1201 ggtgacttcc aaccagcaaa gcggttgcca tgccagcgct gcccctacga agtgattggg
     1261 tcttcctgca ttgatatgaa ttccccataa attctcagga caagccgact cggggtcagg
     1321 ttctgccttc gcagcttcgc ctaccgctgc ttgtccgcaa tcctcctcga gcgcatcggt
     1381 cccctccaca ggcgcaaatt ccacacgtcg gcttttggcc caaaccgcct tgcacttgca
     1441 ccggtcaccg ggagtctcct tgtcttcgca gccttccggc tccttgttgt agcagtccgg
     1501 cccctcgatc tgctcggtgc cttcctcgcc catcccttcc ggctcgtcga gttgaccagc
     1561 aggcacacct cgattgcgca actcacgcgc cacactcctc gctcgtcgta acgacaagtt
     1621 ggcgttcatc aaagatgctc cacgcgaatc ggtgtgcccc acaaccttca cccgcagctt
     1681 cggatacctt ttcaggtatt cgacccactt gccaatggtc gcatccgttt cctcgcgatc
     1741 gcgcttcaat gtatccttgt tgtattcgaa aaaaatctcg ccttcgtact cgaaaccccc
     1801 tccgcaataa cgaattcccc tgccctcaag caccttgacg gcaaaatccg tctttggctc
     1861 cacctcggta tgacgatcga cactgtcgcc cctgggagct gccgaaccgt gcgctccgca
     1921 gcccatccaa accgacattg acaggacaac caagagcaac cggacgtacc acacgcaacc
     1981 atcccctacc ctcgcattcg ccgcatcgct ttgttgggct ctcgacccgt ccgcactcgg
     2041 cgatacttta catacgggct caccttgcaa gccgccccat cgaatcgcac aaaagtaccc
     2101 caataaccgg caaacaggct caaccatccc tcatcctccg attggcctcg gctcaaacaa
     2161 gctcccatcg gccaaaatta cgctgaggcc tgcgagaggt caacgaaaaa cacgtcgatc
     2221 caatgggctc ttatctgatg atttgttacg ccacgctcgg ccagtgcatt tcgacgcggt
     2281 gctcaagggg cttgccaaca acaagcgcaa gccctaagcg taggtttcgc gccccttcaa
     2341 ttcgttgagt ggcagtccag cgcgataat
//
LOCUS       DAOSOR010000750         2367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_44975_length_2367_cov_4.260813, whole genome shotgun sequence.
ACCESSION   DAOSOR010000750 DAOSOR010000000
VERSION     DAOSOR010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44975_length_2367_cov_4.260813"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PK710_24460"
     CDS             <1..526
                     /locus_tag="PK710_24460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HOT12957.1"
                     /translation="WVLLYTLDILDAPRVAKRALESTRGMPETVLFARCDDWMRRDML
                     RYLSDEARSAVRMHQRNGDLVAIATGATLPSALPVARELGIEHIIATELEVRDGRLTG
                     KAIEPLAYGDGKLKKAKYLLDQYGIHLQDTTFYSDSATDLPLLMAVGQPVAVNPDPKL
                     SRMAKTRGWPIQRW"
     gene            complement(541..1248)
                     /locus_tag="PK710_24465"
     CDS             complement(541..1248)
                     /locus_tag="PK710_24465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12958.1"
                     /translation="MNFLSHDFILPADSTPLTRLASALPDLWAVLHRKPLPLVVLRTL
                     EASRHSEARQLARGVRSHLAADTAFHGHPSFGQRVAWLAPQLEPLWKGLRHGHLAAHV
                     LVEMILDAWLIERQPMRVDDYYACFSPSRIRLAARWSASDKLMENEVISVIERFSNSQ
                     FLRDYATPEGLLDRFVRLMMHTPFASGTHPDFDGLVRVTRDAISALEAGSEALLEHAR
                     KASDEALERAAQKHARE"
     gene            complement(1245..>2367)
                     /locus_tag="PK710_24470"
     CDS             complement(1245..>2367)
                     /locus_tag="PK710_24470"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT12959.1"
                     /translation="GAERDGDSPLIVAGGPLTFSNPLPLAPFVDAVLIGEAEDELMDL
                     VDRALAAGSKAHRLEQLATMGSVWVPSLHGDRLPQAAICHTEQLPAWAPIRTPHAELR
                     DMFLVEAVRGCSRRCHYCVMRGQKGRGMRIIPKERIMDRIPSEARKVGLVGASVSDHP
                     HIVSVVETLADRGCHVGLSSLRPERLREPLVRALRAAGAKTITTAMDGSSQRLRDLIE
                     RNTTAEHLLLAAQTCRQARIERLKLYLMVGLPGETTRDMEECAALVRELSRLLPISLG
                     ISTFCAKVGTPLQTAPFAGIDVAQRHIKTLRRALAGKAEVRATSIRWAWVEHVLAGGS
                     ASEGLAVFEAERRGGTFSDYRKAFEKLGHTPFSAHPGDD"
BASE COUNT          496 a          735 c          686 g          450 t
ORIGIN      
        1 gtgggtcctg ctttatacgc tcgatatcct cgatgcccca cgggtcgcaa aacgtgcgtt
       61 ggaaagcacg cgaggcatgc ctgaaaccgt gcttttcgcc cgatgcgacg attggatgcg
      121 tcgagatatg ctgcgctatc tcagtgatga ggcacgctcg gccgttcgca tgcaccaacg
      181 aaatggcgat ctggtcgcca tcgccaccgg tgccaccttg ccctctgccc ttcccgtggc
      241 gcgagaactc ggtatcgaac acatcatcgc caccgaactc gaggtccgcg atggcagact
      301 taccggtaag gccatcgaac cgctcgccta cggtgacgga aaactgaaaa aagccaaata
      361 cctcttggat caatatggaa tccacctcca ggacaccacg ttttattcgg acagcgccac
      421 cgacctgccg ttgttgatgg ctgtcggaca accggtcgcc gtcaacccgg atcccaaatt
      481 atcgaggatg gccaagacac gtggttggcc catacaacga tggtaaccga acggctttgg
      541 ctactcgcgg gcgtgcttct gcgccgctcg ctccaacgcc tcatctgacg cctttcgcgc
      601 gtgttccagc aacgcttcgg aacccgcctc cagcgcggaa atggcatcgc gggtgacccg
      661 taccaatccg tcgaaatccg ggtgagtccc cgaagcgaaa ggcgtgtgca tcatcaatct
      721 cacgaaacga tcgagcaagc cttcgggggt tgcgtaatcc cggagaaatt gggagttcga
      781 aaatctctcg atcacggaaa ttacctcatt ttccatgagc ttatcgctgg cgctccaccg
      841 ggccgccagc cggatcctcg atggcgaaaa gcaagcgtag tagtcgtcga cacgcatggg
      901 ttggcgctcg atgagccacg catcgaggat catttcgacc agcacgtggg cggcgagatg
      961 accgtggcgc aaccctttcc acaacggttc gagctgcgga gcaagccatg ccacccgctg
     1021 gccgaaggac ggatgaccat gaaacgccgt atcggcggcc agatggctgc gtacaccgcg
     1081 agcgagttgt cgagcctcgg agtgtcggga agcctcgagt gttcgcagca ccacgagggg
     1141 caaaggcttt cggtgtaaga cggcccaaag gtccggcagg gcgcttgcca aacgggtgag
     1201 aggcgtcgaa tccgcgggca aaatgaagtc gtgggataag aagttcaatc gtcccccgga
     1261 tgggccgaaa acggcgtatg tccaagtttt tcaaaggctt ttcgataatc cgaaaacgtg
     1321 cctccccggc gttctgcctc gaagacggcg agcccttcgg acgcgcttcc cccggccagg
     1381 acatgctcta cccacgccca tcgaatgctg gtggctcgta cttccgcctt ccccgccaac
     1441 gcgcgccgta acgttttgat atggcgctgg gccacatcga ttcccgcgaa gggcgctgtc
     1501 tgtaatgggg taccgacttt cgcacaaaac gttgatatgc cgagggaaat gggcaacaga
     1561 cggctcagtt cccgcacgag cgcggcgcat tcttccatgt cccgtgtggt ctcgccgggc
     1621 agccccacca tcaggtatag cttgagccgt tcgattcgcg cttgcctgca ggtttgggcc
     1681 gccaacagca ggtgctccgc cgtggtgttc cgctcgatga ggtcgcgaag acgctggctc
     1741 gaaccatcca tggccgtggt gatggtcttg gctcccgcgg cacgcaatgc tcgaaccaaa
     1801 ggctcgcgca gccgctccgg tcgcaggctc gacaagccca cgtgacaacc gcggtccgcc
     1861 agtgtctcca cgaccgatac gatatgagga tggtcgctca cggacgcgcc aacgagccct
     1921 actttccgag cctccgatgg gatacggtcc atgatgcgct ctttcggtat gatgcgcatt
     1981 ccacgacctt tttgccctcg catcacacag tagtggcatc gtcgcgaaca gccccgaacc
     2041 gcctccacga gaaacatatc ccgcaattcc gcatggggcg ttcgaatggg agcccacgcg
     2101 ggcaactgct cggtgtgaca gatcgcggct tgtggcaatc gatcaccgtg caacgaaggc
     2161 acccataccg agcccatggt tgccaattgt tcgagtcgat gagctttcga accagcagcc
     2221 aacgcccggt cgacaaggtc catgagttcg tcttctgctt ctccgatcag aaccgcatcg
     2281 acaaaagggg cgagagggag cggattggag aaagtcaagg ggccacccgc gacgatgagg
     2341 ggggagtcgc cgtctctttc agcccca
//
LOCUS       DAOSOR010000751         2364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_45057_length_2364_cov_3.357731, whole genome shotgun sequence.
ACCESSION   DAOSOR010000751 DAOSOR010000000
VERSION     DAOSOR010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45057_length_2364_cov_3.357731"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2364
                     /gene="dnaE"
                     /locus_tag="PK710_24475"
     CDS             <1..>2364
                     /gene="dnaE"
                     /locus_tag="PK710_24475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOT12960.1"
                     /translation="LPTFDLPEGQDTTTYFIKVAMDGLQQRFDEYRRIGKPVDESAYR
                     QRLEREISVIVEMQFPGYFLIVWDFIRFAKQKKIPVGPGRGSGAGSLVAYAMRITDLD
                     PMPYNLLFERFLNPERVSMPDFDIDFCMDRRDEVIRYVTQKYGADSVGQIAAFHELKA
                     RSVIKDVGRSLGVSAQDTQKLASLVPDLGQGKTATIPQALEIEPKLKAIWELGGIERE
                     VLEQAQRLEGLTRHIGMHAAGVVISNGPLWDTVPAFRQGDTLITQYNMSDVEQAGLVK
                     FDFLGLRTLTVIDETVRRVNMRPDRQGKEPLDINQIPMDDGETFALLQLGETIGVFQL
                     ESSGMQNLFKKLRPDCFEDIIAAVALYRPGPLESGMVDQFVACKHGRQLIKKMHPLID
                     GLLQPTYGVIVYQEQVMQIAQKLANYSLGGADLLRRAMGKKKKEEMDKQTDLFLQGAR
                     DNGVDEHKAKEIFELVAQFAKYGFNRSHSAGYALLTYQTAYLKAHYPAEFLCALMTAD
                     REKTEKVVRIIDEGRAMGVQILPPDINGSDTDFKVVYGSPDGSYRPTGQGRLKDPLQP
                     MIRFGLGAVRGVGDAALEGVFEVRKNGDFKDIFDFCSRVDVRKLNRGVLESLVQSGAF
                     DSTLKPSGVSRAQALASIDVAMERGRSASKDRERGQTTLFGLFDAAVGSTTSSVAEYP
                     DIPSWDLRESCIREKQSLGFYLSSHPLDRYGRSFEKLGAVAVESLVNRDPWAEVRLVG
                     MVESYREKIFKGGGGKSALFDLEDKTGKVTAKVRESQIDKHAPILTSA"
BASE COUNT          562 a          551 c          738 g          513 t
ORIGIN      
        1 cctgcccacg ttcgatcttc ccgaagggca agataccacg acctatttca tcaaagtggc
       61 gatggatggg ctccaacaac gcttcgatga ataccgtcgt atcggcaaac ccgtcgatga
      121 atccgcctac cgtcagcgtc tcgagcggga gatctcggtg atcgtcgaga tgcaatttcc
      181 ggggtatttt ttgatcgtct gggacttcat ccgctttgcg aagcaaaaga agatcccggt
      241 ggggcccgga cgaggctcgg gagcagggtc gctggtggcg tatgcgatgc gcatcacgga
      301 cttggacccg atgccttaca accttctgtt cgagcgattt ttgaatccag agcgcgtatc
      361 gatgccggat ttcgatatcg acttctgcat ggaccgacga gacgaagtca ttcggtatgt
      421 gacacaaaaa tatggtgcgg attccgtggg tcagatcgcg gcatttcatg aactcaaagc
      481 gcgaagtgtg atcaaagatg taggccgatc gctgggcgta tcggctcaag atacgcagaa
      541 attggcgagt ctggtgccgg acttggggca ggggaaaacc gcgacgatac cgcaggctct
      601 cgagatcgag cccaaactca aagcgatttg ggaactcggg ggaatcgagc gcgaagtgtt
      661 ggagcaagcg caaagactcg aaggtttgac acgacacatc ggcatgcatg ccgcaggggt
      721 cgtcatttcc aatggacctt tatgggatac cgttccggct tttcgtcaag gcgacacgct
      781 catcacgcag tacaatatgt ccgatgtcga gcaggcaggg ctggtcaagt tcgacttttt
      841 gggtttgcgc acactgaccg tgatcgacga aacggtgcgc cgtgtgaaca tgcgtccgga
      901 tcgccaagga aaagagcctc tggacatcaa ccagatcccc atggatgatg gggaaacgtt
      961 tgcgttgttg caattggggg aaacgattgg cgtgtttcag ctcgagtctt cgggcatgca
     1021 aaacctattc aaaaaactgc gcccggactg ttttgaagac atcatcgcgg cggtggcgtt
     1081 gtatcgcccc ggacctttgg aaagcgggat ggtggaccag ttcgttgcct gtaaacacgg
     1141 acgacaactg atcaaaaaaa tgcatccgct gatcgatggc ttgctgcagc ccacctacgg
     1201 cgtgatcgtc taccaagagc aagtcatgca aatcgcgcag aaattggcga attattccct
     1261 tggaggagcc gacttgctcc gtcgcgcgat ggggaaaaaa aagaaggagg aaatggacaa
     1321 gcagacggat ctatttcttc aaggagcgcg tgacaatggg gtcgacgaac ataaagcgaa
     1381 agagatcttc gagcttgtgg cgcagtttgc caaatatggc ttcaaccgga gtcattcggc
     1441 gggttacgca cttctgacct atcagacggc ctacttgaaa gcacactatc ccgcggagtt
     1501 tttgtgtgcc ttgatgacgg cagaccggga gaagacggag aaggtcgtgc gcatcatcga
     1561 tgaggggcga gcgatgggcg tacagatttt gccgccggac atcaacggtt cggatacgga
     1621 cttcaaagtg gtgtatgggt cacccgatgg tagctatcga ccgacggggc aggggagatt
     1681 gaaggacccg ctgcaaccga tgattcgatt tggccttggg gcggtgcgtg gcgtgggaga
     1741 cgcggcgctg gaaggagtgt tcgaggttcg taagaatgga gatttcaaag atatttttga
     1801 tttctgttct cgtgtggacg tgcgaaaact caatcgtggc gtgctcgaat cgttggtgca
     1861 aagcggcgcc tttgacagca ccttgaaacc ctcgggcgtt tccagagccc aagcgctggc
     1921 atcgatcgat gtggccatgg aacgtggacg aagcgcgagc aaggataggg agcgagggca
     1981 gacgacgtta ttcgggttat ttgatgcggc agtcggtagt acaacctcat cggtggctga
     2041 atacccggat attccttcgt gggatttgcg cgagtcctgc attcgggaaa agcagtccct
     2101 cgggttttat ttgtcgagcc atccgctcga tcggtatgga cggagctttg aaaaactcgg
     2161 agcggtggcc gtcgaatcgc tcgtgaacag ggacccgtgg gcggaagttc ggttggttgg
     2221 catggtggaa agctatcgcg agaagatttt caagggggga ggggggaaaa gcgccctttt
     2281 cgatctggag gataagaccg gaaaggtaac ggcaaaagta cgagagagtc agattgacaa
     2341 gcatgcgccc attttgacat cggc
//
LOCUS       DAOSOR010000752         2360 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_45140_length_2360_cov_3.894143, whole genome shotgun sequence.
ACCESSION   DAOSOR010000752 DAOSOR010000000
VERSION     DAOSOR010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45140_length_2360_cov_3.894143"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="PK710_24480"
     CDS             complement(<1..1008)
                     /locus_tag="PK710_24480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12961.1"
                     /translation="MGYRIPGTIGLIVLFAALVRCGSDGDEGNAGSAGQSGVGGKAGG
                     DSTGGNAGSGAAAGSAGSGAGPIEIENSGCPQALQPLAARGKVSRVGDGTPASCTEAS
                     LKNAIDSSISKEQWTTIEFDCGKETHTIVVERALRYQGKLMIDGGGRIVLQGAGKDRL
                     LDMDHHSELVVQRLTLRDGVTDASGGAIHHPWYGTLVAVDVRFENNRASLDAGEIGGG
                     AIFAGGLREVIISGCSFLGNRASNGGALLNRGSTLTVVDSLFENNEATSYNSSGQFGN
                     GGGLYIDGMAYEDVGPAGDFHLCGSVFHANRAKQHGSAVFGYFYAGTTAYIDRCQMSN
                     NS"
     gene            1390..>2360
                     /locus_tag="PK710_24485"
     CDS             1390..>2360
                     /locus_tag="PK710_24485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12962.1"
                     /translation="MTIHPIPSFMAECVMPHHDAIRRIVRSGRTIASGFATSEPSALY
                     DILWDYIQNEDITDLDIRQSLFMAPHRLCLGDALTCRGLFDGMAEKSGMGMFSGLARK
                     AQAITKKIEGLQKLVAHYAELRERRIRFTSPFIGATVNMIIPDNAMTRLAFPELVGRN
                     TTRMGITDMQSVHFPEAVDPIGYPEGKPVIDAFVCVMTPPDEHGQMSHGPANGANGEL
                     LDRVLEGGDVDLMVYVNPRYPFTWGYGDASNTIHVDRFRDLARKGRLVVVEDETPVPS
                     LPKDSFSNPSPVELAIAQHVVNHIELHAQSTYGRAIQVGFGGTGVLA"
BASE COUNT          483 a          650 c          691 g          536 t
ORIGIN      
        1 tgagttgttg ctcatctggc agcgatcgat ataggcggtg gttcccgcat aaaaatagcc
       61 gaagacggcg ctgccatgtt gtttggcacg gttggcgtga aatacggaac cgcataaatg
      121 aaagtccccc gcgggtccca catcctcgta ggccatccca tcgatataca atccgcctcc
      181 attgccaaat tgcccggagg agttgtagct ggtggcctcg ttgttttcga agaggctgtc
      241 gacgaccgtg agcgttgaac cgcggttgag aagggcgcca ccgttggaag cgcgattgcc
      301 aaggaacgag cagccagaaa tgatgacctc ccgcaaccca ccggcaaaaa tcgcgcctcc
      361 gccaatctca cccgcatcga gactcgctcg attgttctcg aatctcacat ccaccgcaac
      421 caacgtcccg taccacggat ggtgaatggc acctccggat gcatcggtaa ccccgtcgcg
      481 caaggtcaat cgctgcacca cgagttcgga atgatgatcc atgtcaagca aacgatcctt
      541 gccggcccct tgcaaaacga tccgaccacc cccgtcgatc atcagtttgc cttggtaccg
      601 cagggcccgt tcgacaacga tcgtatgtgt ctctttccca cagtcgaatt cgatggtggt
      661 ccattgttcc ttcgagatgc tcgaatcgat ggcatttttc agggaagcct ccgtgcagga
      721 agccggggta ccgtcgccca cgcggctcac ctttccgcgt gccgcaagag gttgcagggc
      781 ctgggggcat cctgagtttt cgatctcgat cggaccggca ccactgccgg cgcttcccgc
      841 ggcggccccg ctgcccgcgt ttcctcccgt ggaatcgcca cctgcttttc cccccacgcc
      901 gctttgtccc gcggaaccgg catttccctc atcgccgtcg cttccgcaac gcaccaacgc
      961 ggcgaaaagg acaatcaatc caatggttcc tggaatacgg taccccatgg cggacattct
     1021 ccttcgaggc aaggagccgt tttcgatggc tgagccctca tcaagctttc gcaagctatg
     1081 gcgtatacac caaccttgag ttgccaggac cgtccgagca cggcatttat gtcgtcgctt
     1141 gtttctttga ttccttcaga tgtgtgtaac atcttgaagt tattatataa atattgacga
     1201 tggtgccggg ggggcgcggt cgaggcggaa tgacgaacaa cgaaacgcgt ctttcgaagc
     1261 ccgacggtgg cgtcgttggt gggggcatgg gttgtggcgg tcgttggcca agcgtgcgat
     1321 cggctggtgt attcgcgaaa tcgacatttt tttgaggttg tgccagagca gcgggcctgg
     1381 agaccccgta tgaccatcca tccgattccc tctttcatgg ccgagtgtgt gatgccgcat
     1441 cacgatgcga tccgacgaat cgttcgctcg ggtcgaacca ttgcgtcggg gttcgcgacc
     1501 tcggagccat cggcgttgta cgacattcta tgggattaca ttcaaaacga ggacattacc
     1561 gaccttgata ttcggcagtc gttgttcatg gcgccgcatc gtttgtgctt gggtgacgcg
     1621 ctgacgtgcc gcggattgtt tgatggcatg gctgagaaat caggaatggg gatgttttcg
     1681 ggcctggcga gaaaagcgca ggccattacc aaaaaaatcg aaggattaca aaagcttgtg
     1741 gcgcattacg ccgaactgcg ggagcgacga attcgtttca cgagcccgtt cattggtgcg
     1801 accgtcaaca tgatcatccc ggacaacgca atgacgcgtc tggcgtttcc tgagcttgtg
     1861 gggcgcaaca cgacgcgcat ggggattacg gacatgcagt cggttcactt tccggaagcc
     1921 gtcgatccga ttgggtatcc ggaaggcaag cctgttatcg acgcatttgt gtgtgtgatg
     1981 acgccgccgg atgaacatgg gcagatgagc cacggtccag ccaatggtgc caacggtgag
     2041 ttgctcgatc gggtgcttga agggggagac gttgatctga tggtatacgt caatcctcgg
     2101 tatccgttta cgtggggata tggggatgcc tccaacacca ttcacgtgga ccgattccgg
     2161 gatcttgcgc gaaaaggccg gttggtggtg gtggaagatg agacaccggt tccatcgttg
     2221 cccaaggata gtttttcgaa cccatcgccg gtcgagttgg cgatcgctca acatgtggtg
     2281 aaccatatcg aattgcatgc tcaaagcacg tatgggcggg cgattcaggt ggggttcggg
     2341 ggcacgggtg tgcttgcgat
//
LOCUS       DAOSOR010000753         2358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_45186_length_2358_cov_3.532349, whole genome shotgun sequence.
ACCESSION   DAOSOR010000753 DAOSOR010000000
VERSION     DAOSOR010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45186_length_2358_cov_3.532349"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..1421)
                     /locus_tag="PK710_24490"
     CDS             complement(180..1421)
                     /locus_tag="PK710_24490"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOT12963.1"
                     /translation="MKYWPAIVSALVALAVAIPVIQELVPGIRLFERVEATPVQQEQT
                     SAVSREAVGDVEVDRLFIGETGVTAPAGPGRSAHLTLDPKVQRAADAAVKARRIPHGG
                     SVLMDVRTGEILAHVSRRGGNDKSDVITTATVVGTDIFKLVTAAALVNVAGATASTPI
                     CHRDAGKKLELEDLIEDEQKDTWCPSLAEVLARNLDAPLARMAYRELDKEPLEKTAQA
                     FGFGTPVPFDLPVQASRFSVPSTDEGLIRAALGAESVTMSAMQGLVLASVVASQGMMV
                     QPVLVRKVEAAGGRVVYAAPKQPRYLRRVLSQTTAEELKKMMFETTEIGEGFRSFHDD
                     TGHPVFGEVKVAGVVGAEAVVKDAEQVTWFVGFAPVQEPQVAIAAVVRNRRGASGHAT
                     ALAVDILRGYFESRKGDAPNT"
     gene            1523..2266
                     /locus_tag="PK710_24495"
     CDS             1523..2266
                     /locus_tag="PK710_24495"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HOT12964.1"
                     /translation="MPDEERFFRQMRVSGIGPEGQAALSKSRVLVVGCGALGSVLASF
                     IVRAGVGTVHLVDDDWVELSNLYRQILFDQEDALQRRDKALVVASKLSLVHPFVTVKA
                     HRIRLLDDNIDALVGGVDVVVDGTDNFETRYRINDTCVRLGIPWVYGGVLGMTGMSMT
                     VVPGQTPCLRCVFPSVAGAQDGPTPEREGVFGPVVGVVASLQAAEALKLLMGHPPSAG
                     LARVDLMEGSLSRLDLGGRRPDCSCGQSS"
BASE COUNT          478 a          803 c          602 g          475 t
ORIGIN      
        1 tgagtcccgc aaccagcgca aatgccaggt gaatgcggct cggagccacg ctttgcccct
       61 cgacagccac ggaatgttcg ggctgcgatg gctcgcgaga tagttctgtc gagtcatcca
      121 tcgcgttgcg acatacccct cagcgcgata ccgcgcaacc atcggtgacc caaccaccgt
      181 caagtattcg gcgcatcacc ttttcgagac tcaaaatatc ctcgcaaaat atccacggca
      241 agcgccgtcg catgaccact cgcccccctg cgattgcgca ccacggccgc tatcgccacc
      301 tgcggctcct gcaccggcgc aaaccccacg aaccacgtca cttgttcggc atccttgacc
      361 accgcctcgg cccccaccac gcccgccacc ttgacctcac caaaaaccgg atgccccgta
      421 tcgtcatgaa atgaccgaaa cccctcccct atctccgtcg tctcgaacat catcttcttc
      481 agctcttcgg ctgtcgtttg ggaaaggaca cgacgaaggt accgcggttg cttcggcgcc
      541 gcatacacca cccttccccc agccgcttcc accttacgca ccaacaccgg ttgcaccatc
      601 atcccttgac tcgccaccac cgaagccaaa accaacccct gcatcgccga catcgtcaca
      661 gactcagccc ccaacgccgc tcgtatcagc ccttcatccg tggaaggaac cgaaaaccga
      721 ctcgcttgga cgggaagatc gaaaggcacc ggtgtaccaa aaccaaacgc ttgcgccgtc
      781 ttctcgagtg gctccttgtc caactcccga taggccatac gcgccaacgg cgcatccaga
      841 ttgcgcgcca gcacctccgc cagcgaagga caccacgtat ccttttgctc atcctcgatg
      901 agatcttcaa gctccaactt ctttcccgca tcacgatgac aaatgggcgt cgatgccgtc
      961 gcacccgcca cattcaccaa cgccgcggcc gtcaccagct tgaaaatatc cgtgccaaca
     1021 accgtcgccg tagtgatcac atcacttttg tcgttgcccc cacgacgact cacatgggca
     1081 aggatttcgc ctgtccgcac atccatcagc accgatcccc catgcggaat tcgacgcgcc
     1141 ttcacagccg catccgccgc acgctgaacc ttcggatcca acgtcaaatg cgcacttcgc
     1201 cccggccccg cgggagccgt cactcccgtc tcccctataa acagcctgtc cacctccacg
     1261 tcccccaccg cttcccgcga cacagccgag gtttgctctt gttgcacagg ggtcgcttcc
     1321 acacgctcga acagccgaat gcctggtacc agctcctgaa tcaccggaat tgcgacggcg
     1381 agcgcgacca gggcagacac gatcgcgggc caatacttca tggtacgaat acacttacat
     1441 agtaagtaaa tgtagtcaag tttttatctt tcctcccacg aacccccttg gcgacgacgc
     1501 gagggcaacg caagctgctc ctatgcccga cgaagaacga ttttttcggc aaatgcgcgt
     1561 gtctggcatc ggaccggaag gccaggccgc gttgtccaaa agccgcgtcc tcgtggtcgg
     1621 ctgtggcgcc ttgggcagcg tgctcgcaag cttcatcgtc cgcgcaggcg tgggaacggt
     1681 ccacctcgtc gatgacgatt gggtcgaact cagcaacctg tatcgacaga ttctcttcga
     1741 ccaagaagat gctctgcagc gtcgtgacaa ggcgctggtc gtggcatcca agctgtcgct
     1801 tgttcatcct ttcgtgacgg tcaaggcgca tcgaatccgg ctcctcgacg acaatatcga
     1861 tgcgttggtg ggtggagtgg atgtcgtcgt ggatggaacg gacaacttcg agacgcgcta
     1921 tcgaatcaac gacacatgcg taaggcttgg aattccttgg gtttatggtg gtgtcctggg
     1981 gatgacgggc atgtcgatga ccgtggttcc cgggcagact ccttgtttgc ggtgcgtgtt
     2041 tccttccgtg gctggagcgc aagacgggcc aacgccggag cgggagggcg tgtttggtcc
     2101 cgtggtgggg gttgtggcgt cgttgcaagc ggctgaagcc ctgaaactac tgatgggtca
     2161 ccctccttct gccgggttgg cgcgggtgga tctgatggaa ggaagtcttt cgcggctcga
     2221 tctcggcggt cggcgtcccg attgttcctg cggccaatcg agctgattca ccgcagaaac
     2281 gacttatcga tcgtctccct gtcgaagcgc ttccactcgc ccatcacacg cctttcatgc
     2341 ggtaaaaacc gcgccttg
//
LOCUS       DAOSOR010000754         2345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_45522_length_2345_cov_3.189520, whole genome shotgun sequence.
ACCESSION   DAOSOR010000754 DAOSOR010000000
VERSION     DAOSOR010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45522_length_2345_cov_3.189520"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1369
                     /locus_tag="PK710_24500"
     CDS             <1..1369
                     /locus_tag="PK710_24500"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT12965.1"
                     /translation="QRRVAIKFLLPEMLRSTEVVARFQREARAAAMLESEHVTKVIDV
                     GTTPDGTPFMVMELLTGEDLATLVERGGPLSADTAIAYILQACEAIAEAHRAGIVHRD
                     LKPSNLFLAARNDGSSTIKVLDFGISKLQSTTASLALTQESAVMGSPTYMAPEQVKSA
                     KSVDARADIWGLGVILYELLAGKTPFPGTEYHEVLAKVLLEPCPPLTAYRPDISPQLE
                     AVVLRCLQKKPSDRFPTVMDFALALRPFVTDDLTRMSIDRVCRLAGVPAAASTTDTRS
                     VHQPRPQTASNTFVALAATSPAVGTTSPRRIPRGIVFGVGAALLALVLAVGGLWMNWS
                     TTSPPPGGVVPDGFSSSAVSGTSVRASRAPDDPVDAPAESQVLAASAAPTIIVVDPEP
                     TQVASTTSHANQLPVPARSNPSGKSSSGKKSQSSKHPVVGETPPKTAKPATTSRKNPL
                     DMDLK"
     gene            1381..>2345
                     /locus_tag="PK710_24505"
     CDS             1381..>2345
                     /locus_tag="PK710_24505"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOT12966.1"
                     /translation="MRQHKFRNYFAAFLVLASTAGPALGQPAPSAETGTEGDSRAAVR
                     PLAETLTEQALAEYEAGKLLFGDGDFAGASVKFQRAYEISKDGRLLWNKAVCEKNLRH
                     YAAVLRLVRRYQQEAGPMLSEADQQEAADLIHAVSNFVSPVWVTIDETGATISVDGEV
                     IGTSPLTEPFLVDMGSRQITVSKPGFRDYTQTVQVAGMSELRLSIRMDRESVEGRLLI
                     AAGPGETIFLDGKVVAEGRWDGNVSPGRHNIRVTAPGKEPYDSDVEVRAKETRELRIS
                     LSSLSKNDGGGGGSTWLWVTGGTLLVAGAVVGGYFLFKPEDKPAP"
BASE COUNT          492 a          700 c          694 g          459 t
ORIGIN      
        1 ccagcgaagg gtagccatca agttcttgtt gccggagatg cttcggagca cggaggtggt
       61 ggcgcggttt cagcgggaag cgcgagccgc ggcgatgctc gaaagcgagc atgtgacgaa
      121 agtgattgac gtgggaacca cgcctgatgg caccccattc atggtcatgg aacttttgac
      181 tggggaggac ttggccacgc tggtggaacg tggtggcccc ctttccgcag ataccgcgat
      241 cgcctatatt ttgcaggcct gtgaagcaat cgccgaggcc catcgcgccg gtatcgtcca
      301 ccgcgacttg aaaccctcga atctgtttct cgctgccaga aacgatggga gcagcactat
      361 caaagtgctt gattttggga tatcgaagct gcaatcgacg accgccagtt tggctttgac
      421 ccaagaatcg gcggtcatgg gatcgccaac gtatatggcc cccgaacaag tcaaaagcgc
      481 gaaatccgtg gacgcccggg cggatatttg ggggctgggg gtgatcctat acgaattgtt
      541 ggccggaaaa acgcctttcc ccggaaccga gtaccatgag gtgctcgcca aagtgctgtt
      601 ggaaccatgc cctccgctga cggcctaccg tccagatatt tcgccacaac tcgaggccgt
      661 cgtgctgcgc tgcctgcaaa aaaaaccgtc ggatcgattc cccacggtca tggatttcgc
      721 tcttgctctg cgaccgttcg tcacggatga cctcacgcgc atgtccatcg accgcgtctg
      781 ccgattggcg ggcgtccccg cggcggcttc gacaaccgat acaaggtcgg ttcatcagcc
      841 gcggccgcaa actgcatcga ataccttcgt ggctttggct gccacctccc ccgccgtggg
      901 caccacatca ccgcgtcgta tccctcgagg cattgttttc ggcgttggcg cggccctgct
      961 ggcactcgtc cttgccgtcg gaggcttgtg gatgaattgg agcacaacgt cgcctccccc
     1021 cggcggagtc gtgcccgacg ggttcagctc cagcgccgtt tccggaacct cggtgcgtgc
     1081 cagccgtgcg cccgacgatc ctgtcgatgc cccagcggaa tcgcaggttt tggctgcttc
     1141 cgccgcaccg accatcatcg tcgtcgatcc ggagccaacg caagtggctt cgacgacatc
     1201 gcacgcaaat caactccccg tgccggctcg ctcaaatccc tctggcaaat cctcatcggg
     1261 aaagaaaagc cagtcttcga agcatcccgt cgtgggagaa accccgccca aaaccgccaa
     1321 acccgcgacg acgagccgga aaaacccttt ggatatggat ctgaaataag ggaaataccc
     1381 atgcgccaac acaaatttcg taattatttc gctgctttcc tcgtgctggc ttccacggcg
     1441 ggacccgccc tggggcagcc tgctccctcg gcggaaacag ggacagaagg cgattcccgc
     1501 gctgccgttc ggcctttggc cgaaacgctc accgaacagg cgctcgcgga atacgaagct
     1561 ggcaagctct tgttcggcga tggcgatttt gccggtgcca gcgtgaagtt tcagcgcgcc
     1621 tacgaaatct ccaaagacgg acgattgctg tggaacaaag ccgtatgcga gaaaaacttg
     1681 agacattatg ccgccgtatt gcgtttggtg cgccgatacc agcaggaagc tggcccgatg
     1741 ctgtcggaag ccgaccaaca agaagcggcg gacctcattc acgcggtcag caacttcgtg
     1801 agccccgtat gggtgacgat cgatgaaacc ggcgcaacca tctcggtgga tggcgaagtg
     1861 atcggcacat cgcctttgac cgaaccgttt ctggtggata tgggctcgcg ccaaatcacg
     1921 gtctcgaaac ccggatttag ggactacacc cagaccgtgc aggtggccgg catgagcgaa
     1981 ctgcgcttga gcatccgaat ggaccgcgaa tcggtggaag gaaggcttct catcgccgcg
     2041 gggcctggag aaaccatttt cctcgatgga aaggtggttg ccgaaggacg ctgggacggt
     2101 aacgtatcgc ctgggcgcca caacattcgt gtgacggctc ctggcaaaga accctacgat
     2161 agcgatgtcg aagtgcgcgc gaaagaaacc cgggagcttc ggatcagcct ttcttcttta
     2221 agcaaaaacg atgggggggg aggcggcagc acctggcttt gggttaccgg cggaaccttg
     2281 ctcgtggccg gagcggtggt cggcggatac ttcttgttca agccagaaga taaacccgcc
     2341 cctat
//
LOCUS       DAOSOR010000755         2338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_45690_length_2338_cov_5.521244, whole genome shotgun sequence.
ACCESSION   DAOSOR010000755 DAOSOR010000000
VERSION     DAOSOR010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45690_length_2338_cov_5.521244"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..649)
                     /gene="ruvA"
                     /locus_tag="PK710_24510"
     CDS             complement(38..649)
                     /gene="ruvA"
                     /locus_tag="PK710_24510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOT12967.1"
                     /translation="MIGRLTGALVQKDAEGGILLDVHGVGFELTVPRGTLERLGLEQE
                     DRVTLFVHTHVREDALVLFGFSSAEERQAFRVLLGVTSIGPRLALSVLSVLPAAELAK
                     AIRDKDVRSLVAVPGVGKKSAERLILELQDKLGFVTVASAAMGGKKVPMGEESVRQQV
                     VDGLTRLGFKPSEAEWAVSSLGKSVEDRSIPAVIREALALLRH"
     gene            951..1178
                     /locus_tag="PK710_24515"
     CDS             951..1178
                     /locus_tag="PK710_24515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12968.1"
                     /translation="MVVRILSIASVVSICAACGGSGSETPPPVEPVSPKISSQKSPPT
                     SSETVEQETQLRPTPSSIVAPSESDDTDTEP"
     gene            1508..>2338
                     /locus_tag="PK710_24520"
     CDS             1508..>2338
                     /locus_tag="PK710_24520"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOT12969.1"
                     /translation="MTQSVYVVSAVRTPIGSFQGALSSVSAPRLGAVVITKALERAKL
                     RPEDVQEVFMGNVVGAGIGQAPARQAAIFANLPASVPCTTINKVCGSGMQAVIFGTKT
                     LRLGDADIVVAGGIESMSRIPYYLPAARDGMRMGDGKVVDGMIFDGLWDPYGNQHMGM
                     FGEKCAKEFGFTREAQDAFAVESYRRALEAQKQGKFDAEIVGVEVPQRKGEPVVVQVD
                     EEPARGNPSKMAQLRPAFAKDGTITAANASKIDDGACAMVLATDEAVKKHGLVPLARV
                     V"
BASE COUNT          543 a          740 c          591 g          464 t
ORIGIN      
        1 tggccaggtc aaggacgctg cacacaatcg tgtgctttca gtgacgaagc aaggccaagg
       61 cttcgcgaat caccgccgga atcgaccgat cttccacaga ttttccaagg gaggaaacag
      121 cccattccgc ctccgatggt ttgaatccaa ggcgcgtcag accgtccacg acctgctgac
      181 gcaccgattc ttcccccatc ggcactttct ttccccccat cgcggcactg gccaccgtta
      241 cgaaccccaa cttgtcctgc aactccaaga tcaaccgctc cgcggatttc ttacccaccc
      301 caggaaccgc caccaaagac cgaacgtctt tgtcccgaat cgctttcgcc aattccgccg
      361 ccggcaatac cgacaggacc gataaggcga gacgaggccc aatgctcgtc acgcccagca
      421 atacgcggaa ggcttgtcgt tcctccgcgg agctgaaacc aaataggacc agcgcatcct
      481 ctcgcacatg cgtatgaacg aaaagcgtaa ccctgtcttc ttgctccaac cccagccgct
      541 cgagcgtgcc ccgcggtacc gtcagttcaa aaccaacccc atgaacatca agcaaaatac
      601 cgccttccgc atccttttgc acaagcgctc ccgtcaaccg accaatcatg ccctccttta
      661 tcaaccatcg ctcgccaatt gtcagccact gatctgaaac cttccatgaa tacctccaat
      721 ttgcccaaat gttatagcgt ccttcctttt tgttccccgc ttcgaaagag gaggacacca
      781 caagcaccgc ccaccatggc ttgcatcacc aatcgagttg tctccgacgc gtcgaacgcg
      841 gaatctgcat ccatcggtgg attcgtgttc gaaggttatc ggaaagatcg atgaaccacc
      901 gatctcaata ggcttgcccc gcattcacca gacacctaca acctcaaagc atggttgttc
      961 gaatcctatc gattgcttcg gtcgtatcga tatgtgccgc gtgtggagga agtggaagcg
     1021 aaacccctcc ccccgtcgag cccgtatctc ccaaaatatc gagccaaaaa tcaccgccaa
     1081 cctcatcgga aaccgtcgaa caggaaaccc aactccgtcc cacccccagt tcgatcgtcg
     1141 ccccctcgga gtccgacgac acggacaccg aaccttgaag cgtagcgacg ctggtagcga
     1201 tggcgtaacc gtacacagga gcatgctctg ccctgcgcaa cccgcgtcag gatcggttct
     1261 gacgattcga aggatgttct ttgatagcgc tgtccaggcg tagcccatca tcatcacttc
     1321 ggttgacgga aacgaaatcg cagtcccttg cggttcgaca cggagccctc atgcgttgca
     1381 ccaacactcg ccatcaacgg tggaaaacgg tgccacgacg gtccttgcac gagtgggttt
     1441 cagcctcgcc ttcttcgcgg tatggcggtt tcctattccg aatccagcca cgaaggagag
     1501 taggcccatg acgcaatccg tgtatgttgt cagcgctgtt cgtacaccta tcggcagctt
     1561 tcaaggggca ttgtcatccg tttcggcccc gcggctcggt gccgtcgtca tcaccaaggc
     1621 tctcgagcgc gccaagcttc gtcccgaaga cgtacaagaa gtgttcatgg gcaatgtggt
     1681 cggcgcggga attggacaag ccccagcgcg tcaagcagcg atcttcgcga atctccccgc
     1741 atccgtgcct tgcaccacga tcaacaaagt atgcggttcc ggcatgcaag cggtcatttt
     1801 tggaaccaaa accctacgcc ttggcgatgc cgatatcgtc gtggccgggg gcatcgaatc
     1861 gatgtctcgc atcccttact acttacccgc cgctcgcgat ggcatgcgga tgggagatgg
     1921 caaggtcgtc gatggaatga tcttcgatgg attgtgggat ccttatggca accaacacat
     1981 gggaatgttc ggggaaaaat gcgccaagga gttcggattc acacgcgaag cgcaagacgc
     2041 tttcgccgta gaaagctacc gacgagcgct cgaggcgcag aaacagggta agttcgatgc
     2101 ggagattgtc ggagtcgaag tccctcaaag aaaaggagag cccgtggtgg tgcaggtaga
     2161 cgaggagccg gcccgaggaa atccttcgaa aatggcacag ttacgccccg ccttcgccaa
     2221 ggacggcacc atcacggccg cgaatgcttc caagatcgac gacggcgcat gcgcgatggt
     2281 gttggccacc gacgaagctg ttaaaaaaca tgggctggtg cctctcgcca gggtggtc
//
LOCUS       DAOSOR010000756         2322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_46121_length_2322_cov_4.190560, whole genome shotgun sequence.
ACCESSION   DAOSOR010000756 DAOSOR010000000
VERSION     DAOSOR010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46121_length_2322_cov_4.190560"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1440)
                     /locus_tag="PK710_24525"
     CDS             complement(76..1440)
                     /locus_tag="PK710_24525"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOT12970.1"
                     /translation="MVDPKERWERSEGREGDFSQRVTFTYMIGVYLAVNAISDLYLLV
                     EGPDCSYMKTQYVQGNHDWMSTLTSVSGFHRVANTALHPSQMGSSREEGIREVLLRMA
                     GDASVPAIALTSMPMAFITGADYERLTRLVHKQTGKSAVHIPGKSLSGDWLDGYAETL
                     LALASQVELQLEGQQDHRVAIVGYLWDRNEGDHEGNLRELRRIFDALGLELVSVWLDG
                     SRFEDLARVAQAKTILSFPYGRKAAKKLARRTNARLIEMPLPFGFEDSERFVRRLGEA
                     FACETQAEAFIDRQLSTIIPKLEWVVPFLFQNRRFAFVGEPHILPGFEGLVSMLGGEL
                     AYAVITNHPHHASTLRVGLEASCVLVYPKMKEMVRFLFGKARETDIHLVVTNNVGIMG
                     AEGAIFEFGFPSSFRHALYDRPFLSFAGALAFVDSMANTMRMHEVELARRTMSMGLGK
                     LLGH"
     gene            complement(1433..>2322)
                     /locus_tag="PK710_24530"
     CDS             complement(1433..>2322)
                     /locus_tag="PK710_24530"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOT12971.1"
                     /translation="KAVEADERSVNLIGFAGAKAVEELREALREFGVGVNVVLLPDLN
                     VDRVDALPRARTNVFYPNQLWRHMYDQVLDRSSLKAVEVPGPFGFEASRAWFRQVAQA
                     VGESDEAKQERAWEKVVAPWQSRWENLCAQARSYRLGFVVRDEEAYYLTNPGASWGVP
                     LLELVEEMGFGIDVLVGVSDAKIAKNAALAIRRRFRKPDRHSVRAFDSFSFLRKRLRD
                     CPANAFLSYHFYDWRLTEAGKASFSIQHFEMGVAGAVRTIERLLRVCQTTFFSRYGRY
                     LGREESGLRTVGQKEKLDG"
BASE COUNT          575 a          769 c          506 g          472 t
ORIGIN      
        1 cgcggctgtg gtcgacgcgc cgatccgaag cgctcaccat caaagtcacc atgtcgactc
       61 tcgtagcctg gcgcttcaat ggcccaatag cttgcccaaa cccatcgaca tcgttcttcg
      121 cgctagctca acctcgtgca ttcgcatcgt gttcgccatc gagtcgacga aagccagcgc
      181 tcccgcgaag ctcaaaaatg gtctgtcata caaagcgtgc cggaacgaag agggaaaccc
      241 aaactcgaag atggcacctt ccgcacccat gatgcccacg ttgttggtca ccaccaaatg
      301 aatgtccgtc tcgcgcgctt ttccgaataa gaaacgcacc atctctttca tttttggata
      361 gacaagcacg caagaagcct cgagccccac ccgcaacgtc gacgcatggt gcggatgatt
      421 cgtgatcacc gcgtaggcca attcgcctcc tagcatcgat accaacccct cgaaaccagg
      481 caagatgtgc ggctccccca caaaggcaaa ccgacgattc tgaaacaaaa agggaacgac
      541 ccattcgagt tttggaatga ttgtcgacaa ctgccgatcg atgaacgctt ccgcctgagt
      601 ctcacatgca aatgcttctc ccaaccgtcg aacgaatcgt tccgagtcct cgaatccaaa
      661 gggtaacggc atctcgatca aacgtgcgtt cgttcttcgt gcaagcttct ttgcagcctt
      721 tcgtccatag ggaaacgaca agatcgtttt cgcttgcgca acacgcgcaa ggtcttcaaa
      781 ccgggaacca tcgagccaaa ccgatacgag ttccaaccca agcgcgtcga atatccgcct
      841 tagttcacgc agatttccct catgatcccc ttcgttgcga tcccataaat accccacgat
      901 cgccacgcga tggtcttgtt gcccctcgag ctgcaactct acttgcgagg ctagcgcaag
      961 cagagtctcc gcataaccat ccagccaatc cccagacagc gacttgcccg gtatgtgaac
     1021 cgccgacttg cccgtttgct tgtggaccaa acgcgtaaga cgctcgtaat ccgcgcccgt
     1081 gatgaacgcc atgggcatcg atgtcagcgc tatcgcgggt acggaggcgt cacccgccat
     1141 tcgaagcagc acctcgcgaa tgccttcttc acgggaactg cccatctggg aagggtgcag
     1201 cgccgtgtta gccactcggt gaaagcccga cacactcgtc aacgtggaca tccaatcgtg
     1261 attgccctgc acgtattgcg tcttcatata gctgcaatcc ggcccctcga ccaaaagata
     1321 caaatccgaa atcgcattga ccgcaaggta aacgccaatc atgtacgtga acgtcacccg
     1381 ttggctgaaa tcgccttcgc gcccctcgga tcgctcccat ctctcctttg gatcaaccat
     1441 ccaacttttc cttttgcccc accgttcgca acccgctttc ctcgcgacca agataacgcc
     1501 catagcgcga gaaaaatgtc gtttgacaca cgcgaagcaa tcgctcgatc gtgcgtaccg
     1561 cgcccgcaac ccccatctcg aagtgctgaa tcgaaaaact cgccttaccc gcttcggtca
     1621 agcgccaatc ataaaagtga taggaaagaa atgcgttcgc tgggcaatcc cgcagtcttt
     1681 ttcgcaaaaa cgaaaaacta tcgaaagcac gcaccgaatg ccggtcgggt tttcggaacc
     1741 gacgacgaat cgctaacgct gcatttttag cgattttcgc atcacttacc cccaccaaca
     1801 catcgatccc aaaccccatc tcttccacca attcgagcaa aggcaccccc cacgacgctc
     1861 caggattggt gagataatag gcttcttcgt ctcgcaccac aaaaccaaga cgatacgatc
     1921 tagcttgagc acataagttt tcccaacgcg attgccacgg cgccacgact ttttcccagg
     1981 cacgctcttg ctttgcctca tccgattcac ccacggcttg cgccacttgt cgaaaccacg
     2041 cgcgggaagc ttcgaatcca aaaggacccg gcacttccac agccttgagc gaactgcgat
     2101 ccaatacctg atcgtacatg tgccgccaaa gttgattcgg ataaaaaaca ttcgtccgcg
     2161 cgcgcggcag cgcatccacc cgatcgacat tcaagtccgg aagcagaacc acgttcaccc
     2221 caacaccaaa ctctcgcaac gcctcccgca actcctcgac cgccttggcc cccgcaaaac
     2281 caatgagatt caccgagcgt tcatcggctt caaccgcttt cg
//
LOCUS       DAOSOR010000757         2317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_46252_length_2317_cov_3.343059, whole genome shotgun sequence.
ACCESSION   DAOSOR010000757 DAOSOR010000000
VERSION     DAOSOR010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46252_length_2317_cov_3.343059"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PK710_24535"
     CDS             <1..442
                     /locus_tag="PK710_24535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOT12972.1"
                     /translation="IAHELHLLGVPLLPRMITEHAHGATGIDIHPGAQIDRRFFIDHG
                     TGVVIGETCVIGRGVRLYQGVTLGAKSFPLDENGKPIKGIARHPILEDDVIVYSGATI
                     LGRITIGRGSVIGGNVWLTRGVPPNSRIAQQAPRQEVFVDGGGI"
     gene            complement(506..1381)
                     /locus_tag="PK710_24540"
     CDS             complement(506..1381)
                     /locus_tag="PK710_24540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12973.1"
                     /translation="MSLKPITRRFYAPVALVGVMATVFPTVSRADDVQACIEAHEHAQ
                     VARKEGKLLDARQSLLTCVRDTCPTILQKECAPWLTEVNSSIPTISVIARRADGTELY
                     EVRVLVDGRQVASTLDGRAIEVDPGPHVLRFETDGLPPVEQETVFREGIKNHEVMVVF
                     ESTSSGPPAPLEPTPDVDRDTIRPVPTMVYVLGGVGIAGLATSVYFEARGLSKRSDLD
                     AQGCKPFCSTSDVDAAKRDILIGDIALGAGLVSLGAATYFYLSRPSVPTSTASVQPSL
                     DLRKHTAMAFVQGSF"
     gene            complement(1378..>2317)
                     /locus_tag="PK710_24545"
     CDS             complement(1378..>2317)
                     /locus_tag="PK710_24545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12974.1"
                     /translation="DRRADIYAASVVLWELLSNRKLFKGQHDVSLMHEILERTSNAAI
                     EPPSQRTPGLPKSLDNIVLQGLSARPDDRWQSARDMAIAIEKALVVASTREVGDWVRE
                     EVGDELREKADKIAEFESLTSQPAFEVHELLSQPTASSGISVISAPNTGVISSLPTPG
                     GTTIVSQQSSSSGRHVVILSAGVAIGIIGLGIIGAYTLVHSATSTVSSDSPHATAPPS
                     ATIQEETPTIQIDQLMRVTEDADVVVEEPDSQEPEASARPVVRPPVIKPAGNKDKPPP
                     SPTKPPAGTTPPAAQCDPPFTVDANGIKRFKPECLR"
BASE COUNT          482 a          605 c          710 g          520 t
ORIGIN      
        1 aatcgcccat gaattgcatc tgcttggcgt gccgttgctg ccgcgcatga ttacagagca
       61 tgcgcacggg gccacgggca tcgacattca cccaggcgca caaattgacc gacgcttttt
      121 catcgaccat ggaacgggag tcgtgatcgg tgaaacgtgt gtcatcggac gaggggttcg
      181 tctgtaccag ggggtgaccc ttggagcgaa gagctttcct ctcgatgaaa acggtaagcc
      241 catcaaaggg atcgcacgtc atccgattct cgaagacgat gtcatcgtat attcgggagc
      301 tacgattctt ggtcgaatca cgatcggtcg cggctctgtc atcggaggca atgtatggct
      361 gacccgtgga gttccgccca atagccggat tgctcaacaa gcgcctcgac aggaagtatt
      421 cgtcgatggt ggaggaatct aatcgacgcg ccctcgtcgt cggttttccc ttgaatcaca
      481 gagggaacaa ccatggctcc aagcttcaaa acgagccttg tacgaaagcc atggcggtgt
      541 gttttcgcaa gtccaggctc ggttgcacgc ttgccgtgga agtgggaacc gaggggcgag
      601 ataagtaaaa ataggtagcc gcgccgagcg agacgagtcc tgcccctagc gcgatatccc
      661 caatcaatat gtcacgtttg gcagcatcca cgtcgctcgt ggagcagaac ggtttgcatc
      721 cctgcgcatc gaggtcggag cgtttcgaca gtccccgcgc ttcgaaataa acggaggtgg
      781 caagccccgc gatcccaacg cccccgagca cataaaccat ggtgggcacg ggtcgaatgg
      841 tgtctcgatc gacgtcagga gtaggttcca agggggctgg tggaccgctg gacgtcgact
      901 cgaacacgac catgacttca tggtttttaa ttccttcacg aaacaccgtc tcttgttcga
      961 cgggcgggag tccatcggtt tcgaagcgaa gaacgtgagg tcccgggtcc acttcgatcg
     1021 cgcgaccgtc gagcgtggag gccacctgtc ggccatcgac gagcacgcgc acctcatata
     1081 gttcggttcc atccgcacga cgtgcaatga ccgagatggt ggggatgctg ctgttgactt
     1141 ccgtaagcca cggggcgcat tccttttgaa gaatcgttgg gcaggtatct cgaacgcacg
     1201 taagcaagct ctgccgtgca tcgagaagtt tcccttcttt tctagctact tgcgcgtgct
     1261 cgtgggcttc gatgcaggcc tgtacatcat ctgcccgaga taccgtggga aatacggtag
     1321 ccatgacacc aacaagggcc acaggggcat agaagcgtcg agtgataggt ttgaggctca
     1381 ccttaagcac tccggtttga aacgtttgat gccattggca tcgacggtaa acgggggatc
     1441 gcattgtgct gcggggggcg ttgttccggc aggtggtttc gtgggagaag gtggtggttt
     1501 gtccttgttt cccgcgggct tgatgacagg cgggcgaacc acgggacgcg cggaagcttc
     1561 gggctcctga ctatcgggct cttcgacaac gacgtctgcg tcttccgtca ctcgcatgag
     1621 ctgatcgatt tgtatcgtcg gcgtttcttc ttggattgtg gcggacggtg gcgccgtcgc
     1681 gtgaggggaa tcgctactca cggttgaagt tgctgaatga acgagagtgt acgcaccaat
     1741 gattccgaga ccgatgattc caatggcaac acccgcggaa aggatgacga catggcgtcc
     1801 ggacgatgag gactgctggg agacaatggt cgttccgcct ggtgttggca aagatgagat
     1861 gacgccggtg tttggagccg atatcactga aatgccggaa gaggcagtgg gttgagatag
     1921 tagttcgtgt acttcgaagg cgggctgtga ggttagactc tcgaattccg caattttgtc
     1981 agccttttcg cgtaactcat cgccgacttc ctcacgaacc cagtcaccga cttcgcgcgt
     2041 ggaagcgacg acgagggcct tttcgatggc gatggccata tcgcgcgcgc tttgccatcg
     2101 atcgtcgggg cgggccgaaa gcccttgaag cacgatattg tcgaggcttt tggggagccc
     2161 tggcgttcgt tggcttggag gttcgatcgc tgcgttgctc gtgcgttcga ggatttcgtg
     2221 catcaacgaa acgtcatgct gtcctttgaa cagttttcga ttgctcaaaa gttcccacaa
     2281 cacgacagaa gcagcgtaga tatccgcgcg ccggtcg
//
LOCUS       DAOSOR010000758         2307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_46541_length_2307_cov_4.151421, whole genome shotgun sequence.
ACCESSION   DAOSOR010000758 DAOSOR010000000
VERSION     DAOSOR010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46541_length_2307_cov_4.151421"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..767)
                     /gene="wbpD"
                     /locus_tag="PK710_24550"
     CDS             complement(183..767)
                     /gene="wbpD"
                     /locus_tag="PK710_24550"
                     /EC_number="2.3.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003113426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2-acetamido-3-amino-2,
                     3-dideoxy-D-glucuronate N-acetyltransferase"
                     /protein_id="HOT12975.1"
                     /translation="MTPPLIHPSAIVDDGAQIGTGSRVWHFVHVCAGARIGQGVSLGQ
                     NVFVGNRVVIGDRCKIQNNVSVYDGVTLEEGVFCGPSMVFTNVYNPRSLIERKHEYRA
                     TLVKKGATLGANCTILCGITIGSYAFVGAGAVVNQDVPDYALMVGVPARQIGWMSEYG
                     ERLDLPLEGHEHAVCPHMGAKYELRGRVVRKLSA"
     gene            complement(1158..1550)
                     /gene="trxA"
                     /locus_tag="PK710_24555"
     CDS             complement(1158..1550)
                     /gene="trxA"
                     /locus_tag="PK710_24555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOT12976.1"
                     /translation="MATITLTEDNFNETIENNDIVLIDFWAPWCAPCRAFGPVFEKAS
                     EKNTDAVFAKINTEEQQGLAAAFNIRSIPTLMVFREKVLIFSQPGMLPAPVLDELMGK
                     IRELDMDEVRAKIAEEEKKSQGAEAAPN"
     gene            1699..>2307
                     /locus_tag="PK710_24560"
     CDS             1699..>2307
                     /locus_tag="PK710_24560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12977.1"
                     /translation="MSNGSLLASAGVVMMAWSLGCGCPCPEPATPQAPAAAAARTRPT
                     LPPGALRQQCADTPAESTSPTIEVFADLGVIRLSADTFVDLPVQQRKLAYALAKVAQA
                     ATPIQYEQRHPQGKHIQILAEELALHLNAVPADLRPSVKTFVALVFAHKGPRDAWSDE
                     RLSFSLTRAQLETAVLAAWKAGASFPGVSSEPALRTWLSKLTP"
BASE COUNT          475 a          639 c          691 g          502 t
ORIGIN      
        1 gtgccgttgg cgacgctgat gcaaaagcgg gcgccggtat agacggcaag tttttcttcc
       61 agttcggcca cttccggtcc gaggatgaac tgaccgtggt ccaacacctt ctggatgttg
      121 gtgtcgattt gcgcttttac ccgttggtat tgggctttca gatccacgaa ttgcatgggt
      181 cctcaggctg ataatttgcg aacgacgcgg cccctgagtt catatttcgc gcccatatgg
      241 gggcagacgg catgttcgtg gccttccaaa ggaaggtcga gtcgctcgcc gtattcactc
      301 atccagccga tttgacgagc tggcactcct accatgagcg catagtccgg cacgtcttgg
      361 ttgaccaccg ctccagcccc gacgaaggcg taggaaccga tggtaatccc gcaaagaatg
      421 gtgcaattcg cgccgagggt ggcgcccttt ttgaccaacg tggcgcggta ttcatgcttg
      481 cgctcgatga gcgaacgcgg attgtagaca ttggtgaaca ccatgcttgg gccgcaaaaa
      541 acaccttctt ccaacgtaac gccatcgtaa acgctaacgt tattttggat tttgcatcgg
      601 tcaccgatga cgacccgatt gccgacaaaa acgttttgac cgagggaaac cccctggccg
      661 atgcgtgccc cggcgcaaac gtggacgaag tgccagacac ggctgccagt gccgatttgc
      721 gcgccgtcat cgacgatggc gctgggatga atcaggggag gcgtcatttc aaggggattc
      781 atgttttttg gataccgccg caaattagac aagaaaaact gcgagcgctt gaccctatcc
      841 tcgactgcct tggcacggga ttcggcctcg agcacgggcc tacgtctgta atcattcgtc
      901 gagttggtat gcaagaaaga acgacggctt gcgcgcggga gccatggtca cggttcgaag
      961 acaacgcaat gggtggcggg agcgagtgtg ccgcttcgcg tcgcccctcg ggatgcgatg
     1021 ggtgtgctga tcgaagtgga ttgagaagga ctggcgcggc gggcccaacg gcatcccagg
     1081 ggggattgac gatgggtaga gcgtgcccac tgccagggcg cgcagaacat aactattgga
     1141 aatataaaga tattttatca attcggagcg gcttcggccc cttgagattt cttttcttcc
     1201 tccgcgatct tggctcgcac ttcatccatg tccagctctc ggatcttgcc catcaattcg
     1261 tcgagcacgg gggctggcag cattccgggt tgggagaaga tgaggacctt ttcgcgaaac
     1321 accatgagcg tgggaatgga tcggatgttg aaggccgcgg ccaggccttg ctgttcttcg
     1381 gtgttgattt tggcgaagac cgcatcggtg tttttctcgg aagctttttc gaaaaccggc
     1441 ccgaacgcgc gacagggagc acaccaggga gcccaaaagt cgatgaggac gatatcgtta
     1501 ttttcaatgg tttcgttgaa gttgtcttcg gtcaaagtga tggtcgccat catgctactc
     1561 cccgcgcctc gcgcgtgttt gaatccgctt gttgggattg gagcaagccg ccccacaaag
     1621 gattcgcaag ccgaaaaaag atgagaaccg aggggtgaat cgcttgcgaa tcgtgcaggg
     1681 aggtgctggg cttcgtgcat gtcgaacggt tccctccttg ccagcgcggg agttgtcatg
     1741 atggcctgga gtctgggatg tggctgtcct tgccccgagc ccgccacacc gcaagctcct
     1801 gccgctgctg ctgcgcgcac acgacccacc ctaccacccg gcgccttacg gcaacaatgc
     1861 gcggacacac ctgctgaatc cacgtcaccc acgatcgaag tgttcgcgga cttgggcgtg
     1921 attcgattgt ccgcggacac ttttgtggat ctaccggtgc agcagcgcaa gctggcctac
     1981 gcgctggcga aagtggcgca agcggcaaca cccatccaat acgagcaaag gcacccgcaa
     2041 ggaaagcaca tccaaatcct ggccgaagaa cttgccttgc acctgaacgc cgttcctgcc
     2101 gatcttcggc catcggtcaa gacattcgtg gcgctagtat ttgcgcataa aggcccgcgg
     2161 gatgcgtgga gtgacgagcg tttgtcgttt tccctgaccc gggcacagct cgaaacggcg
     2221 gtgctggcag cgtggaaagc tggtgccagc tttccgggcg tgagttccga acccgccttg
     2281 cgaacgtggc tgtcgaagct gactccc
//
LOCUS       DAOSOR010000759         2301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_46722_length_2301_cov_3.782725, whole genome shotgun sequence.
ACCESSION   DAOSOR010000759 DAOSOR010000000
VERSION     DAOSOR010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46722_length_2301_cov_3.782725"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="PK710_24565"
     CDS             <1..1206
                     /locus_tag="PK710_24565"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOT12978.1"
                     /translation="VINNSWGPGGGDPNFAAVSFPIPAASSVVKAAFTYAETHGRNGK
                     GTVVVFAAGNENEMVSAYGKEPTMVTVAAVDDQGLKAYYSNRGPQVLVAAPSNGGIRG
                     IVTTSAGSQYTDSFGGTSSASPFVAGVAGLILSANPDLTAAQVRDILAQSATKVDPVW
                     GDYDENGHSPYYGYGLVNAAVAVRLASGSCTDPADCPAPSDDCGTNCSKAACDACRTT
                     ADCEQGLVCQALPPLGRSVCVAGEAAGCPAGTSAHRGYCIPTRSSCGLCAASETCNGR
                     DDDCNGQVDENLDCTGVRVLQCPLADEGCSASEACAATVCVPSCQTDADCGEDANCRI
                     VKDRYGKANSLVKGCAKSLVGTCKSGCDILASTMTDDQIEDFVSCMKDGKTSCMAAFG
                     CASKLPIKM"
     gene            complement(1322..>2301)
                     /locus_tag="PK710_24570"
     CDS             complement(1322..>2301)
                     /locus_tag="PK710_24570"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT12979.1"
                     /translation="RLLRTHEYDQLQRTETLRLMFGLPLEEPRPPISQRLQSAGYHTI
                     ASLDDGYSEFLSEKSGVHKGFSVFRHVDSLPKEKRNDVGNTELALAALRSRPPDKPFM
                     LWSHYFGPHDPSTKHNEVQSFGSSVTDLYDHEILFADHALSPLLEELDAIGKQRPLAV
                     FVTSDHGEYLTSTRRVHGSGLHELTTHIPLIAKIPGVSPGNSDALVSLIDIPATILRL
                     CGVRGAAGMDGVDLREVLTGKHKHRVLHSDTWRFKVNGEPWFDLTSAYDDQHKLLFFR
                     HTNQMRLERRDDPNPQTTNLLDEIPIPKHLDDAMGAYMEQTGGPPDLHD"
BASE COUNT          468 a          524 c          774 g          535 t
ORIGIN      
        1 gtgatcaaca atagctgggg acctggggga ggagacccga atttcgcggc cgtttctttc
       61 ccgattcctg ctgcatcgtc ggtggtcaaa gcggcgttta cgtatgcgga aacgcatggt
      121 cgcaacggca aaggaacggt ggtggtattt gcggcgggca atgaaaacga gatggtttct
      181 gcttatggca aagagccgac gatggtgacc gtggcggccg tggacgatca gggattgaaa
      241 gcgtattaca gcaatcgagg tccgcaagtg ctggtggcgg cgccttccaa cgggggcatc
      301 cgcggcatcg tcacgacgtc ggcgggttcg caatacacgg attcttttgg gggaacgtca
      361 tcggccagtc ccttcgttgc gggagtcgcg gggctcattc tttccgcgaa ccccgatctg
      421 accgcggcgc aagtacgcga tattcttgcg caatctgcga ccaaggtcga tccggtgtgg
      481 ggtgattacg acgagaacgg tcattcccct tactacggat atgggctggt gaatgcggcg
      541 gtggcggtgc gtcttgcttc gggaagttgt accgatccgg ccgattgtcc ggcgccctcg
      601 gatgattgcg gaacgaattg tagcaaagcc gcctgcgatg cttgtcgaac gacggcggat
      661 tgtgagcaag ggctcgtctg tcaggccctt cctccgctgg gacggtccgt gtgtgtggca
      721 ggcgaggctg cggggtgccc tgcgggaacg agtgcgcatc ggggctactg cattcccacg
      781 cgaagcagtt gtggactatg tgctgcctcg gagacttgca atggtcgaga tgacgattgc
      841 aacggacagg tggatgaaaa cctggattgc acgggtgtgc gggtgcttca gtgtcctttg
      901 gccgatgaag ggtgctcggc ctccgaggct tgtgcggcaa ctgtctgtgt tccctcgtgt
      961 caaacggatg cggattgcgg cgaagatgcc aattgcagaa tcgtcaagga tcggtatggg
     1021 aaggcgaaca gtctggtgaa gggatgtgcg aaatccttgg tgggcacatg caagtccggt
     1081 tgtgacattc ttgcatcgac gatgacggat gatcagatcg aggatttcgt ttcctgcatg
     1141 aaggatggca aaacctcatg tatggccgcg tttgggtgcg catcgaaact gccgatcaag
     1201 atgtgaaaaa tggctttgtc ggagggtgta gatgccagcg atcgcaagag ccttcgcagg
     1261 ggatagcgtc gttcgtgcga aggcttctgc ggtgaaacgt cagcgtgatg ggtaagggga
     1321 atcaatcgtg tagatcgggg ggcccaccgg tctgttccat ataagcaccc atggcatcat
     1381 cgagatgctt gggaatgggt atttcatcaa gtaagttagt ggtttgtggg ttgggatcgt
     1441 cgcgacgttc caatctcatt tggttggtat gtcgaaagaa aagcagtttg tgttgatcgt
     1501 cataggcgga tgtgagatcg aaccaaggtt ctccgttgac tttgaaccgc caggtatcgc
     1561 tgtgtaaaac ccgatgcttg tgttttccgg tgaggacctc acgcaggtcg acgccatcca
     1621 tgcccgcggc gcctcgcacg ccgcatagtc gcaggatggt tgcggggata tcgatcaggc
     1681 tgacgagagc atcgctattc cctggtgaaa cgccaggaat tttcgcgatc aacgggatat
     1741 gggtggtgag ttcatgcaac ccggaaccat gcacgcgacg ggtactggtc aggtattcgc
     1801 cgtggtcgga ggtgacgaaa acggcaaggg ggcgttgttt tccgatggca tcgagttctt
     1861 cgagcaaggg gctaagtgca tgatccgcaa agaggatttc gtgatcgtac aagtccgtga
     1921 ccgaggagcc gaaggactga acttcgttgt gtttggtact ggggtcatgg ggtccgaagt
     1981 aatggctcca gagcatgaac ggtttgtctg gtggtcggct gcgcagtgct gcgagggcga
     2041 gttccgtatt gcccacgtcg tttcgttttt ctttgggcaa ggaatccaca tgacgaaata
     2101 cggagaatcc cttatgaacg ccggattttt cgcttagaaa ctcggagtaa ccatcgtcca
     2161 aggaggcgat ggtatggtag ccggcggatt gcaatcgttg tgaaatgggc ggccgcggct
     2221 cttcgagggg aaggccaaac atgagtcgaa gggtttcggt tctctgaagt tggtcgtatt
     2281 cgtgggtgcg aagcaggcgg t
//
LOCUS       DAOSOR010000760         2297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_46825_length_2297_cov_4.447368, whole genome shotgun sequence.
ACCESSION   DAOSOR010000760 DAOSOR010000000
VERSION     DAOSOR010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46825_length_2297_cov_4.447368"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PK710_24575"
     CDS             complement(<1..658)
                     /locus_tag="PK710_24575"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT12980.1"
                     /translation="MIEAIPLLHDYLHLSARRLPDKTALVCQKKRLTYSEIEARSNAL
                     AWSLEKNGVQRGDRVIVFADNTVETVIAFWAVLKANAVVSIVNPLTKADKLAYLLDDC
                     RAVAMMTDAHLTRVFADVVKRAKHLRTLIVSGTSDLSQFDTIACVKSWDDAVGEGNTD
                     CPPRRQCIDIDLAAIIYTSGSTGDPKGVMLTHRNMITAATSVSTYLRFAQDEIVMCVL
                     P"
     gene            complement(668..>2297)
                     /gene="asnB"
                     /locus_tag="PK710_24580"
     CDS             complement(668..>2297)
                     /gene="asnB"
                     /locus_tag="PK710_24580"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOT12981.1"
                     /translation="ALWDTKTETLVLARDWAGIVPLYVCVHDNRLYFASELKVIFAAD
                     PTIPRDLDPVGMHETFTFWTIVPPQSVFRGIEEIEPGHVRTIHLGVIRDQPFVEPSYR
                     TGPEDGFRGTLDDAVRAVGVALEKAIRIRMDRADVAVGCYLSGGLDSSLVASMANEAH
                     KGKPQALRTFSLRFEDAEYDETPFQQEMVERMGSDHSEVVVSRHAIAEAFRDVIWFTE
                     RPILRTAPVPLILLSRRVREMGMKVVLTGEGADEVFAGYDIFREAKVRRFWAKSPQSE
                     CRPRLLEKLYPYLARSPVAQQAMSRRFFGRNLDQAHRPGFSHDMRWEGTSALKRLMSP
                     EFSSVGVDVVARVLDSLPASFDRWSFLSRDQYLEIRILLAGYLLSCQGDRMLMANSVE
                     GRFPFLDNDVVRLANSLPADYKLRVLDEKHVLKRLASGKIPEAIVRRKKQPYRAPDAL
                     SFVGPGAPEWIEDMMSEAAVRDAGVFSPAAVSTLWRKCKTRANDGQFSNTDNMAVVGV
                     LSTQVLHHTFVRSNPKPESIVEFNTLIDRLSPHR"
BASE COUNT          540 a          662 c          614 g          481 t
ORIGIN      
        1 gaggcaacac acacatcacg atctcgtcct gcgcaaagcg caaatacgtg gatacactgg
       61 tcgctgccgt gatcatgttg cgatgcgtga gcatgacgcc tttggggtca cccgtgcttc
      121 ccgaggtgta gatgatggcc gcaaggtcga tatcgatgca ctgccgacgc ggcggacagt
      181 ccgtgtttcc ttcgcccacc gcatcgtccc acgacttcac acacgcgatc gtgtcgaatt
      241 gcgaaaggtc cgacgttccc gacacgatga gcgttcggag gtgtttcgcc cttttgacca
      301 catcggcaaa tacccgcgtg agatgcgcgt cggtcatcat ggccaccgct cggcaatcat
      361 cgagcaaata cgctagtttg tcggctttgg tcagcggatt gactatggat acgaccgcgt
      421 ttgccttgag cacagcccag aatgcaatca ccgtctccac cgtattgtcg gcgaatacga
      481 tgacacgatc gcctcgctgt accccatttt tttccaaaga ccaggcgagc gcattggatc
      541 gcgcctcgat ctcggaatac gtcaaacgct tcttttgaca gacgagagca gtcttgtcgg
      601 ggagtcgccg cgccgacaaa tgcagataat cgtgcaagag cgggatcgct tcaatcatcg
      661 caatatccta ccgatggggt gaaagacggt cgatgagggt gttgaactcg acgatcgatt
      721 cgggtttggg attggaacga acgaaggtgt gatgcaagac ctgggtcgat aacactccta
      781 ccacggccat attgtcggtg ttggagaatt gaccatcgtt tgcgcgcgtc ttgcacttgc
      841 gccacaaggt cgagaccgcc gcgggcgaga atacccctgc atctcgcact gcggcctcgg
      901 acatcatgtc ctcgatccat tcgggcgccc cgggacccac gaaagataat gcatccggcg
      961 cacggtaagg ttgctttttg cgtctcacga tggcttcggg aatctttccg gaggccaatc
     1021 gtttgagcac gtgcttttca tccaataccc gaagcttgta gtccgcgggt aaggagttgg
     1081 cgagacgaac gacatcgttg tccaaaaatg ggaatcgtcc ttccaccgag tttgccatca
     1141 gcatgcggtc accctgacag gagagcaggt aaccggccaa taggatccga atttcgagat
     1201 attggtctcg ggatagaaaa gaccaacgat caaatgatgc gggaagggaa tccaacaccc
     1261 ttgccacgac atcgacgccg acggaagaaa actcaggaga catcaaacgc ttgagagcgg
     1321 aagttccttc ccaacgcatg tcatgggaaa agccgggacg atgggcctga tcgagattcc
     1381 gaccgaaaaa gcgtcgagac atcgcctgct gtgcgacagg agaacgcgcc agataagggt
     1441 aaagcttttc gagcaaacgg ggtcgacatt cggattgtgg gctttttgcc caaaatcgac
     1501 gcactttggc ttcacggaat atgtcgtatc cagcaaagac ctcgtcggcg ccctctcccg
     1561 tgagcaccac tttcattccc atttcgcgaa cacggcgtga gagcaggatc aagggcaccg
     1621 gcgccgttcg aagaataggt cgctccgtaa accagatcac atcgcgaaac gcttcggcaa
     1681 tcgcgtgtcg cgatacgacg acctcactgt ggtcacttcc cattcgctcg accatctctt
     1741 gctgaaatgg cgtttcatcg tattccgcat cctcgaagcg caaagaaaac gttcgtaatg
     1801 cctgtggttt ccccttatgc gcttcgttgg ccatcgaggc gaccaaggaa ctgtccaagc
     1861 ccccagatag ataacagccg actgccacat ccgctctatc catgcgaata cgaatggctt
     1921 tttcgagtgc tacgccaacc gcgcgcaccg cgtcgtcaag cgtaccccga aacccatcct
     1981 caggccccgt ccgatacgac ggctccacaa agggttggtc tcgaatcacc cctagatgga
     2041 tggtgcgaac gtgacccggt tcgatttcct cgataccgcg aaacaccgac tgcggcggga
     2101 caatggtcca aaacgtaaac gtctcgtgca ttcccacagg atcgagatcc ctggggatgg
     2161 ttgggtcggc ggcaaagatc actttcagtt cggaagcaaa atacagccga ttatcatgca
     2221 cacatacata gagcgggaca atgccggccc aatcccttgc cagcaccaac gtctcggttt
     2281 tcgtgtccca cagcgcc
//
LOCUS       DAOSOR010000761         2291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_46987_length_2291_cov_3.511181, whole genome shotgun sequence.
ACCESSION   DAOSOR010000761 DAOSOR010000000
VERSION     DAOSOR010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46987_length_2291_cov_3.511181"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PK710_24585"
     CDS             complement(<1..303)
                     /locus_tag="PK710_24585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12982.1"
                     /translation="MSPLDIGYAVALLARHGIPARIIDLEAEADSFEILMKRLREHQP
                     SVLFLHAITPGIPHALRIARYARDHFASLQKIVAIGQHASVDPHSVLDDNSPIDLCL"
     gene            complement(393..1682)
                     /locus_tag="PK710_24590"
     CDS             complement(393..1682)
                     /locus_tag="PK710_24590"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HOT12983.1"
                     /translation="MRKIGPWKLGLVLCMGIACSANSDKGEFGTGAGGNGTQHPQDAG
                     TSGGSVQTGGGPVEDSGGLLLDAQVNTDSEPSNYCPHMDNEDHDGDGFSFEQGDCNDC
                     DPNANPGAYDVFGGVDGGPGVDEDCNGIADDEPIECDDGIDIASNDPMAAARAIGLCR
                     VAVPNATGKDRTWGVLSAKYVKVDGTPGMNDLSHGILPKFGAANVQQGKTMLALSSGT
                     ARAPGQPGFSSPNGAIMGTNSAPPPGYPKESPACPGVVTGPVFDPAALELVVRVPTNA
                     RSFSFNLNFYTFEFPEYICSEFNDFYVTMMWPPPPGLPDANISFDQDNNPISVNNSLL
                     QVCKPQSAGGKNFPCPLGPALLQNTGYAGPLIGSHAATGWLQTRAPVEPGSEITLRFA
                     IWDSGDPVLDSLVLIDNFQWSVDPASGSSTEPVPSPK"
     gene            complement(1860..>2291)
                     /locus_tag="PK710_24595"
     CDS             complement(1860..>2291)
                     /locus_tag="PK710_24595"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOT12984.1"
                     /translation="PRFLKIEITESTAATHVESAIATLQRLKNMGINVLIDDFGTGYS
                     SLSYLQQLPIDMLKIDRSFVKNAVSDKDSAAIVRAIIAMAHGLGIQVVAEGVETLQQL
                     NFLFRERSDFIQGYFFSQPVTAEAFAQLLRQDRHLDYLPHR"
BASE COUNT          502 a          633 c          682 g          474 t
ORIGIN      
        1 caaacacagg tcgatcgggc tattatcgtc gagaaccgaa tgcggatcga cgctggcatg
       61 ttgtccgatc gccacgattt tttgaagaga tgcgaaatgg tcgcgggcgt agcgggcgat
      121 acgaagagcg tggggaattc cgggcgtgat ggcgtgaaga aaaagcacgg aaggctgatg
      181 ctcccgtagc cttttcatga gtatttcgaa actgtcggct tcggcttcga gatcgatgat
      241 ccgcgcgggg ataccatgac gcgccagcag ggcgacggca tagccgatgt cgagcgggct
      301 catgacattt tcgggatggg cggcgcgacg ttcccaccag gaagggaccc gaacgaagac
      361 gatcatgatt gcgaataccg atggggaaaa agctatttag gcgagggtac gggttcggtg
      421 gaggaaccgg aagcggggtc gacggaccac tggaagttat cgatcagcac gagagaatcg
      481 aggacggggt ctccggaatc ccagatggcg aagcgcaggg taatttcgga gccaggttcg
      541 acgggggcgc gggtttgcaa ccatcccgta gccgcatgag agccgataag gggtcccgca
      601 tacccggtat tttggagcaa cgcggggccg agggggcatg ggaaattttt ccctcccgcc
      661 gactggggct tgcacacttg aaggaggcta ttgttcacac tgatggggtt gttgtcttgg
      721 tcgaaggaga tgttggcgtc cggaagccca ggggggggcg gccacatcat cgttacgtaa
      781 aagtcattga attccgagca gatatattct ggaaactcga aggtataaaa gttgaggttg
      841 aaggagaaag accgtgcgtt ggtgggcacc ctcaccacga gttccagcgc agcggggtcg
      901 aatacgggac cggtaaccac cccgggacag gccggtgatt ctttgggata accaggaggc
      961 ggagcgctat tggtgcccat gatcgcgcca ttaggcgacg agaatccagg ctgtcccggc
     1021 gcgcgagctg ttcccgagga aagggcaagc atggtcttgc cttgctgcac gttcgccgcg
     1081 ccaaatttgg gcaaaattcc gtgggacaaa tcgttcatcc ctggcgtccc atccaccttc
     1141 acgtacttgg ccgacagcac gccccacgtt cgatctttgc cggtcgcgtt cggaacagca
     1201 accctgcaca atccaatcgc tctggcagcc gccatgggat cgttgctcgc aatgtcgatc
     1261 ccgtcatcgc attcgattgg ttcatcatcc gcgatgccat tgcagtcttc atcgacaccg
     1321 gggccgccat ccacgccgcc aaagacgtca taagcgcctg ggttggcgtt gggatcgcag
     1381 tcattgcaat cgccttgctc gaacgaaaag ccatcgccat cgtgatcttc attatccata
     1441 tggggacaat aattcgacgg ctcgctatcg gtgttcacct gcgcatcgag cagcaatccg
     1501 ccactatctt ccacgggtcc tccccccgtt tgcaccgatc cacccgaggt tccagcgtct
     1561 tggggatgct gcgtcccatt gccccctgcc cccgttccga actcgccttt atcggagttg
     1621 gcggagcaag cgattcccat acacaacacc aatccaagct tccacggtcc tatcttgcgc
     1681 atctccacct ctcctgaagc caatgagccc aacgcgagca catacttctc aggctagcct
     1741 gcgaaccccc atccagcaag gaatccacgg ctgcggagcc gctcccgcgc atcgacaaac
     1801 cagcaccttg ggtgcaatcc tacacttcga tcggaagttg cgtcgatatc gaccgtcctt
     1861 cacctatgcg gcagatagtc cagatggcgg tcttggcgga gcaactgcgc gaaggcttcg
     1921 gcggtcacgg gctgactgaa aaaataccct tgaatgaaat ccgagcgttc gcggaacaag
     1981 aagttgagtt gttgcagtgt ttcgacgcct tcggccacga cttgaatgcc aagtccatgc
     2041 gccatggcaa tgatcgcgcg aacgatggcc gccgagtctt tgtcactcac cgcatttttg
     2101 acgaaggaac gatcgatttt gagcatatcg atgggcagtt gctgtaagta ggacagcgac
     2161 gaataccccg tgccgaagtc gtcgatgagc acgttgatgc ccatgttctt gagccgttgc
     2221 aacgtggcga tcgcggattc gacgtgggta gcggcagtcg attcggtgat ttcgattttg
     2281 agaaaacgcg g
//
LOCUS       DAOSOR010000762         2287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_47102_length_2287_cov_3.569444, whole genome shotgun sequence.
ACCESSION   DAOSOR010000762 DAOSOR010000000
VERSION     DAOSOR010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47102_length_2287_cov_3.569444"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PK710_24600"
     CDS             <1..420
                     /locus_tag="PK710_24600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12985.1"
                     /translation="KLPMGLILGGVAGLLGLLVTIAFIAYFYVIRQEPEVPAITLPPV
                     ATIAQEDPSPDPSPTNEPTIVPPVTTAEPPSTTPTGTTTVRPRDAGVDAGFVFPTFPD
                     AGIALPPFPSSIPSGLIPPLPTELPTIPLPPWPPSNP"
     gene            417..539
                     /locus_tag="PK710_24605"
     CDS             417..539
                     /locus_tag="PK710_24605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12986.1"
                     /translation="MNIARDRYGLFTEEMLIISTITSPAFAVSWVGQVEQDSDG"
     gene            532..1791
                     /locus_tag="PK710_24610"
     CDS             532..1791
                     /locus_tag="PK710_24610"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT12987.1"
                     /translation="MANLQPGDMIDQRYQMGRLIGSGGMASVYEVLDTTSGHRAAVKI
                     LHRQFMLDPVIGQRFIREAQAASVLDTPYSVKVEGTGNLSDGRPFIVMELLEGLTLQA
                     LIERQGPRFEAERVLYLADQVALGLIDAHARGIIHRDLKPDNIFVINTPAGELIKIVD
                     YGISKIFSGEGGVKLTQTGVTVGTPQYMPVEQLRGTKNIDGRVDVYALGVVLYEMLAG
                     QKPYDGFTYEEVILKVATTTAPSLGVYRSDLPEGLVSLVDRAMARQVQDRIASMTQLR
                     QEMAPYWSGRSPLSGSGQSLAQSGAFVSARSPELASVSPRASAGHTQVLQGPAAALAP
                     PVPAPPPELAAPSPPVPAPQPELPAKTLPDHVYQASRQEPVSAAPPMVETRQKSSSTI
                     VILLVIIAVFLFLLLVGIGVLALMLLG"
     gene            complement(1938..>2287)
                     /locus_tag="PK710_24615"
     CDS             complement(1938..>2287)
                     /locus_tag="PK710_24615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12988.1"
                     /translation="PNPVPSNGPIVLDLEIVVNEPIQAPVALQIALPEGAQLSSGLPS
                     ESLSLLQPGRLYRQYVVQTASPLRYPVVVTAQAKGPNDAWGFNARREYPTSDILPPHP
                     PSPNFGRPPVVRP"
BASE COUNT          451 a          643 c          699 g          494 t
ORIGIN      
        1 aagctaccga tgggactcat tctcgggggt gttgcaggct tgctcggcct tttggtcacc
       61 atcgccttca ttgcgtattt ttatgtgatt cggcaagagc cagaggttcc cgcgatcacg
      121 ctgccgcctg tcgcaacgat tgcccaagaa gacccatcgc ccgacccatc gcccacgaac
      181 gaaccaacca tcgtgccgcc tgtcacgacg gcagagccac cgtcgacgac gcccacgggc
      241 accacgaccg ttcgtccacg agacgccgga gtcgatgctg gatttgtttt ccctaccttt
      301 cccgacgccg gcatcgcatt gccgccgttt ccctcttcga ttccttcggg gctgattccg
      361 cctttgccca ccgaactgcc cacgattccc cttccgcctt ggcctccttc gaacccatga
      421 acatcgcgcg agaccgatat ggccttttca ccgaagagat gttgatcatc tcgacgatca
      481 cttcaccagc ttttgcggta tcctgggtcg gccaggtgga gcaagacagc gatggctaac
      541 cttcagccag gagatatgat cgaccaacgc taccagatgg ggcgattgat cggttctggg
      601 ggaatggcgt cggtgtatga agtcctggac acgacgtcgg gtcatcgtgc ggccgtcaaa
      661 atactgcatc ggcagttcat gctcgatccg gtcattgggc agcgattcat ccgcgaagcg
      721 caggctgcaa gcgtgctgga caccccatat tccgtcaagg tcgagggaac cggcaacctg
      781 agtgatggtc gtccgttcat tgtcatggag ctgctcgaag ggctcacgct gcaagctctc
      841 atcgaacgtc aaggtcctcg tttcgaagca gaacgggttt tatatctcgc ggatcaagtc
      901 gcgttggggc tcatcgatgc gcacgctcgt ggcatcattc atcgggatct caagcccgac
      961 aacatcttcg tgatcaacac ccctgcgggt gagttgatca aaatcgtgga ctacggcatc
     1021 tcgaaaatat tttcgggaga aggcggtgtg aaactcaccc aaacgggtgt gaccgtgggc
     1081 acgccgcaat atatgcccgt cgagcaactg cgaggcacga agaatatcga tggtcgggtc
     1141 gatgtatacg ccttgggcgt ggtgctttac gagatgcttg cggggcaaaa gccctatgat
     1201 ggcttcacgt atgaggaagt catcttgaag gtggccacga cgaccgcgcc ttcgttggga
     1261 gtctatcgta gtgatttgcc cgaagggttg gtcagcttgg tcgatcgagc gatggcgcgc
     1321 caggtccagg atagaattgc gagcatgacg cagcttcgcc aagagatggc gccttattgg
     1381 tccggtcgat ccccactatc cggatcggga cagtctttgg cgcaatcggg agcgtttgtg
     1441 tccgctcgtt ctcctgagct tgcttccgtg tcaccgcggg cgagtgcggg acatactcaa
     1501 gtgctgcaag ggcccgcggc tgcactggcg ccaccagttc ccgcaccgcc gccggaattg
     1561 gcagcaccgt cgccacccgt tccggcgccg cagccggaac ttcccgcgaa aacattaccc
     1621 gatcatgtgt accaggcgag ccgccaagag ccggtttccg ccgcgcctcc gatggtggaa
     1681 acccgtcaga aatcgtcttc cacgatcgtc atcctcctcg tcatcatagc cgtttttctg
     1741 tttttattgc ttgtcggaat cggtgtgctt gccctgatgt tgcttggctg aacaccgaat
     1801 ccatcgatgt ggggtgtatc gatgaaggag ccatcctggt catcgtgatg ccgtggcacc
     1861 tacgatccca ggcgtagtca aacgccgacg agccgatggg aaaggggaga tagcgaaggg
     1921 atgcgggcta ggaacgatca gggacgaacg acgggagggc gtccgaagtt gggacttggt
     1981 ggatgaggcg ggaggatatc cgaagtggga tactcgcggc gagcgttgaa cccccaggcg
     2041 tcgttggggc ctttggcttg tgccgttacg accacggggt atcggagtgg tgatgcggtt
     2101 tgcacaacgt actggcggta caatcggcct ggttgcaaca gggagaggga ttcggagggt
     2161 aacccgctgg acagttgagc gccttcgggc aaggcgattt gcaaggcgac gggggcctga
     2221 attggctcat tgacgacgat ttccaggtcc aagacgatgg ggccgttaga gggtacgggg
     2281 ttggggc
//
LOCUS       DAOSOR010000763         2275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_47420_length_2275_cov_4.440541, whole genome shotgun sequence.
ACCESSION   DAOSOR010000763 DAOSOR010000000
VERSION     DAOSOR010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47420_length_2275_cov_4.440541"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PK710_24620"
     CDS             complement(<1..194)
                     /locus_tag="PK710_24620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12989.1"
                     /translation="MQPVRHWIARSVLALLVMGMAYMVACGDDPSTADYEQDKSDSGN
                     EGYEGYEGYEGYEGYEGYEG"
     gene            complement(465..1895)
                     /locus_tag="PK710_24625"
     CDS             complement(465..1895)
                     /locus_tag="PK710_24625"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT12990.1"
                     /translation="MFADRYPCYVGGEARFFDRQLPVTNKYTQEVFTRVSLAGEGVVD
                     EAIALARQASEPMRRLACYERQAVLDHCVARFRERAEELALSLCLEAGKPIRDARGEV
                     ARLIDTFRLASEESTRIYGEVMPLDISPRARGYTGFWKRVAIGPCSFISPFNFPLNLV
                     AHKVAAALAVGCPFVLKPASFTPLGALLIGEILTETSLPKGAFSILPCSRSAADAFTT
                     DERLKLLSFTGSPEVGWDLKARAGKKKVVLELGGNAAVVVDQGVDLDDVVQRILVGAF
                     YQSGQSCISVQRILIHEAIFEPLRDRLVQATLGLVMGDPREETTFIGPMITEKEAERL
                     ESWIQSAVRRGARVLCGGKRRGNMHEATLLSEVPKDENLVCKEAFGPVAVLSKFKDLD
                     EAFREVNDSAYGLQAGVFTRDIFRAHQAWEELEVGGVVINDIPSWRVDHMPYGGVKDS
                     GLGREGVRFAMQDMTEIRMLVMRTPG"
     gene            complement(1895..>2275)
                     /locus_tag="PK710_24630"
     CDS             complement(1895..>2275)
                     /locus_tag="PK710_24630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12991.1"
                     /translation="LARWKDLGPELLVKYMDGNVRDDQGKVTHPSYPKEHYARIVAET
                     GDHFYLRDESQESAAPSASTLGPASASVAAPPSSGISPPISSAPSPTKAKGGCAVGVS
                     SASGEYAGLLMLLFGWMMKKRRGE"
BASE COUNT          531 a          761 c          516 g          467 t
ORIGIN      
        1 tacccttcgt acccttcgta cccttcgtac ccttcgtacc cttcgtaccc ttcgtaccct
       61 tcgttgccac tatccgactt gtcttgctca tagtcggccg tacttgggtc gtccccgcaa
      121 gcgaccatat aggccattcc cataacgagc aaagccaata ccgatcgcgc tatccaatgt
      181 ctgacaggtt gcatgggacc tcctaagcca cgttttcaaa catagaatgt cataactatg
      241 tgtcgacgtc aacgtggttc aaccggcggg ggtaacgtca ggaccccgtt ggcatgatcg
      301 ccaacggctc cggccgtttt caagggctat ccatcgaaac ccctcattta ttccgatatg
      361 aaccgtgaag tttgtggagg atcgcttacg atactgtaga agctcgattc gcgaatcatc
      421 aatggttccc tattccttcc atcccctcgc tccatccacc gcactcatcc cggtgtccgc
      481 atcaccagca tgcgtatctc cgtcatatcc tgcatcgcaa aacgaacccc ttcccgaccc
      541 aatccgctgt ctttcactcc accataaggc atgtgatcca ctcgccaaga aggaatatca
      601 ttgatgacca cgccccccac ctcgagttcc tcccacgcct gatgggctct aaaaatatcc
      661 cttgtaaata caccagcttg caatccgtag gcgctatcgt tcacctcccg gaacgcctcg
      721 tcgagatcct tgaacttcga caataccgcc accggaccaa aagcctcttt gcacaccaga
      781 ttctcatcct tgggcacctc cgatagcaat gtagcctcat gcatattgcc gcgacgtttg
      841 cccccgcata gcacccgagc cccccgccga accgccgact ggatccagga ttcgaggcgt
      901 tcggcctctt tttccgtgat catcggcccg ataaaggtcg tttcctctct cggatccccc
      961 atcacaaggc caagggtggc ttgtacgagt ctatctcgca atggttcgaa aatcgcttca
     1021 tgaatgagaa tgcgttgcac actgatacag ctctggccgg attgataaaa cgcacccacc
     1081 aagatgcgct gaacgacatc gtcgagatcc acgccttgat ccaccacgac ggcagcattt
     1141 cccccgagtt cgagcacgac cttctttttt ccagcccggg ctttcagatc ccaaccaact
     1201 tccggtgatc ccgtgaaact cagcagcttc aaacgctcgt cggtggtgaa ggcatcggcc
     1261 gcggagcgcg aacagggcaa aatggaaaag gctcccttgg gaagactcgt ttccgtaagg
     1321 atttcaccga tcaaaagcgc gcccaacggt gtgaaactcg ccggtttgag cacgaacggg
     1381 caaccgaccg caagcgccgc cgccacctta tgggccacca ggttcaaagg aaaattgaaa
     1441 ggcgagatga aagaacaagg acctatcgcc acccgcttcc aaaatcccgt gtacccacga
     1501 gcccgcggac tgatgtccaa cggcatcact tccccgtaaa tccgagtcga ttcctccgag
     1561 gcaagccgaa acgtatcaat cagccgagcc acctcccccc gcgcgtcgcg aatcggcttt
     1621 cccgcttcca ggcaaaggct gagtgcaagc tcttcggcac gctcccgaaa acgcgccaca
     1681 caatgatcca ataccgcctg ccgctcgtaa caagcaagcc gacgcatcgg ctccgatgct
     1741 tgccgcgcca acgcaatcgc ctcgtcgacg acgccctcgc ccgccaggga cacgcgcgta
     1801 aatacctctt gcgtatactt attggtgacc ggaagctgcc ggtcgaaaaa acgcgcctcg
     1861 ccgcccacat aacacggata tcgatcagca aacatcattc acctcgacgc tttttcatca
     1921 tccacccgaa taacagcata agcagccccg catattctcc cgacgccgaa gacacaccca
     1981 cggcacaccc tccttttgcc ttcgttggcg aaggtgcgct cgaaatcgga ggggatatac
     2041 cggaagacgg cggcgcagcg accgacgcgg aagcgggccc aagggtagac gcggacggag
     2101 cggccgactc ttgcgactca tcccgcaaat aaaaatgatc ccctgtttcc gcaacgatac
     2161 gcgcataatg ctctttgggg tacgaaggat gcgtgacctt gccctgatcg tcgcgcacat
     2221 tgccatccat atacttgacg agtaactcag gtccgaggtc tttccaccgc gcaag
//
LOCUS       DAOSOR010000764         2251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_48100_length_2251_cov_3.970856, whole genome shotgun sequence.
ACCESSION   DAOSOR010000764 DAOSOR010000000
VERSION     DAOSOR010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48100_length_2251_cov_3.970856"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="PK710_24635"
     CDS             <1..898
                     /locus_tag="PK710_24635"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOT12992.1"
                     /translation="ITRDEDLTPNLHHAGGCGHALQRCGPVWGVRVNAIALGGPALHS
                     GTTSAVVLTHRPGLLALRQRDVEIPFRSLRVDRADYGVTVRGQSLRVDLVEHLFAAIA
                     GLAIVSDLRVDVYGSEIPLLDGGALRFSRALRQLGFASGEPIVTVARAATFRWGESVY
                     RFSPFPTVRIVVNVQFDHALIQGQRACWQGDPQDFLERIAPARTFGFRRDAEQLQAVG
                     RARAVDLRSVVVLDENGTCLSDPPPSPDECVRHKLLDLLGDLMLWGGVPTGHVEVTRP
                     GHRATHSVMGEAWARGVVVDAR"
     gene            1055..>2251
                     /locus_tag="PK710_24640"
     CDS             1055..>2251
                     /locus_tag="PK710_24640"
                     /inference="COORDINATES: protein motif:HMM:NF012563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOT12993.1"
                     /translation="MTYPFHISDDVAGMRKAIENHLKCTLARDARTATRQDWYLATAH
                     AVRDRIMERYIVTMQTHANANTRRVYYLSLEYLMGRMLHNNLYNTGLFETARKALAEL
                     GLAMPEIDEEEPDMGLGNGGLGRLAACFLDSLATLDLPAVGYGIFYEFGLFRQRFVNG
                     HQTEYPDSWLQVPSPWFVMRPEYAIEVKLYGHVEHTVDDRGDFTIRWVGTRTVLGIPH
                     DLPICGYGARTVNFLRLWESRASEEFDLTIFNEGGYVEAVREKAVGETFTKVLYPNDK
                     SESGKELRLVQQYFIVACSLRDIVRRYFRENEGWDQFADKVAIQLNDTHPAVAVAELM
                     RLMIDVYGLEFDEAWSITQRTVGYTNHTLLPEALESWPLPLFERLLPRHMQIVYAINS
                     RVLREAR"
BASE COUNT          426 a          615 c          697 g          513 t
ORIGIN      
        1 gatcacccga gatgaagacc tcacgccgaa ccttcaccac gctggtggct gcggtcacgc
       61 tcttcagcga tgtggcccgg tttggggagt aagggtgaac gccattgccc ttggtggccc
      121 ggcgcttcat agcggaacca cttcggccgt cgtattgacg catcgaccag gccttctggc
      181 gctgcggcag cgagacgtcg agattccgtt tcggagctta cgcgtcgatc gagcggacta
      241 tggggttacc gtgcgtgggc aaagcctccg ggtggacctg gtcgagcatc tttttgccgc
      301 gatcgctggg ttggccattg tctcggatct gcgggtcgac gtgtatggtt cggagatacc
      361 gctgttggat gggggagccc tgcgcttttc gcgcgccttg cgacagcttg gttttgcctc
      421 tggcgaaccc atcgtgaccg tggcacgagc tgccaccttc cgatggggcg agtccgtgta
      481 tcgattcagt ccgtttccaa cggtgcgtat cgtggtgaac gtgcaattcg accatgcgct
      541 gattcaaggc caacgagcct gttggcaggg agacccacaa gattttttgg aacgaatcgc
      601 cccggcaaga acgttcggat ttcgtcggga tgccgagcaa cttcaagcgg tgggacgggc
      661 gcgagccgtg gaccttcgtt cggtcgtggt cttggatgaa aatgggacgt gcctttcgga
      721 cccaccgccg tcccctgatg aatgcgttag gcataagcta ttggatttat tgggagatct
      781 tatgctctgg ggaggggttc ccacggggca cgtggaggtt acccgaccgg ggcatcgcgc
      841 tacccattcg gtgatgggtg aggcttgggc tcgtggagtc gttgtggacg cgcgctaagc
      901 catggttttg ggcctatctt ccctgaacga ggagagcatg gttacaatgg ctgcccaact
      961 tcgtgtcgtt cgcgaccatc atcgggtctt cgatcggcta acgtccgtgt atcaaggctc
     1021 gtgagacagc cccctgaccc tgcaaggacc tatcatgacc tatccttttc atatttctga
     1081 tgatgtggcg ggtatgcgaa aagcgatcga gaaccacctg aagtgtacgc tcgcgaggga
     1141 tgcgcggacc gcaacgcgtc aggactggta tcttgcgacg gcccatgccg tgcgtgaccg
     1201 catcatggag cgatacatcg tcacgatgca gacgcatgcg aatgcgaata ctcgtcgtgt
     1261 gtactactta tccctcgagt acttgatggg gcggatgctg cacaacaatc tgtacaacac
     1321 gggattgttc gagactgcgc gaaaggcgct tgcggagctt ggtcttgcca tgcctgagat
     1381 cgacgaggag gagcctgaca tggggttggg caacggcggt ctggggcggc ttgcggcgtg
     1441 ttttttggac tccttggcca cgcttgacct tcccgcggtg ggctatggaa ttttttatga
     1501 gttcgggttg tttcgccagc gcttcgtcaa cggtcatcag accgagtatc cggactcctg
     1561 gctgcaagtt cccagtccct ggttcgtgat gcggccggaa tacgccatcg aggtgaaact
     1621 atacggccat gtggagcata cggtcgacga tcgaggggac tttaccatcc ggtgggtagg
     1681 tacacgaacg gtgttgggta tcccccatga ccttccaatt tgtggctatg gagcccgtac
     1741 cgtcaatttc ctccgattgt gggagtctcg cgcctccgag gaattcgacc tcaccatctt
     1801 caatgaaggc ggatacgtcg aagccgtgcg cgaaaaagcc gtcggtgaga cgtttaccaa
     1861 agtcctgtat ccgaatgaca aatccgaatc agggaaggaa ctccgtctcg tgcagcaata
     1921 cttcatcgtt gcctgttcgc tacgagacat cgttcgacga tattttcgcg aaaacgaggg
     1981 ctgggatcag tttgcggaca aggttgcgat ccaactcaac gatacccatc cggcggtcgc
     2041 tgtcgcggag ctgatgcggc tgatgatcga cgtctacggc ctcgaattcg acgaagcctg
     2101 gtcgatcacc cagcgcacgg tcggttacac caatcacacg ctgctgcccg aggcgctgga
     2161 aagctggccg ctgccactgt tcgagcggct gctgccccgc cacatgcaga tcgtctatgc
     2221 gatcaacagc cgcgtactgc gcgaagcgcg c
//
LOCUS       DAOSOR010000765         2248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_48190_length_2248_cov_4.129503, whole genome shotgun sequence.
ACCESSION   DAOSOR010000765 DAOSOR010000000
VERSION     DAOSOR010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48190_length_2248_cov_4.129503"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1243)
                     /locus_tag="PK710_24645"
     CDS             complement(146..1243)
                     /locus_tag="PK710_24645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HOT12994.1"
                     /translation="MRHQAALVCLVGLFGSACDSEDPSPAASPDAGVTDSSDAEPPLD
                     AAQDLPEDSPPDQSADAPFDEADAAEPLPSLERRTYAGLEGKRDYLLHRPAGPQDKPL
                     VVVLHGCNQSAASFAKLTGFDLLAEEASFVAVFPEQSGQANPYLCWNWFLEDQQRRDV
                     GEAAILHGIVGEVVAEGGIDPSRIYAIGLSAGGAMAVVLGTVFADVFAAVGTVEGCPF
                     RGTPCLNEPSPLTGNELAQIAYSAMGAHARALPVFVVQGDADAVVKPANGDLVVEQWL
                     GVADLADNGQLDGSVPRTPTTEQTQTAPGGKTYEILTYGEGESVWVLRWTVMGMAHAW
                     SGGASGMLFSDPEGPDAARGAWDFFSKHVLP"
     gene            complement(1324..1773)
                     /locus_tag="PK710_24650"
     CDS             complement(1324..1773)
                     /locus_tag="PK710_24650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12995.1"
                     /translation="MTETINSHVQQAIGAWKKLIDKQQEMAGSVTDELMSWNKKGVEK
                     LSERLEQAKQLSSEVMEHAREISDPTEMVSTWSGMVDRHLEAVSGSTKSMMDLQAEGW
                     DKARATLDTFHKASTTTFDVGMKVHKAWCDAVMETAKRSADWTPVGS"
     gene            complement(1899..>2248)
                     /locus_tag="PK710_24655"
     CDS             complement(1899..>2248)
                     /locus_tag="PK710_24655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12996.1"
                     /translation="ASKLLPVSLLVQLIRLGDASLAEFLGRYLSVALALYLQAKRGAS
                     VVSSFNPFASVPFEAISAFARMFASSVPYQEPKVTAEPVEEGEPSDFHPEVAALRREI
                     EELRKEIQQKHKT"
BASE COUNT          473 a          647 c          654 g          474 t
ORIGIN      
        1 agcctggcgc ccacgcagcg atcgatggaa tcacaggtaa acacgggagt tcgatagacg
       61 tcccgagcaa ttcgttcgct tgcttcgcgc acgtcttcga aggtgggtat ccggcatggg
      121 ccggatttgg gagtccaggc gttcatcagg gcagcacgtg cttggagaag aaatcccagg
      181 cgccccgcgc ggcatcaggc ccttcgggat cggaaaagag cattcccgaa gctcctccgg
      241 accaggcatg ggccatcccc atcaccgtcc aacgaagcac ccacaccgac tccccttccc
      301 catacgtcag tatttcgtat gtttttcccc caggagccgt ctgcgtttgc tccgtggtgg
      361 gcgtccgcgg caccgaacca tcgagctgcc cgttgtccgc gaggtccgcg acaccgagcc
      421 attgttccac gacaagatcc ccattcgcgg gtttgaccac cgcgtctgca tccccctgca
      481 ccacgaaaac aggtagtgcc cgggcatgcg ctcccatggc cgaataggca atttgggcaa
      541 gttcattgcc cgtgagcggc gaaggttcgt tcaggcaggg agtgccgcga aacggacacc
      601 cctccaccgt ccccaccgcg gcaaaaacat cggcgaaaac cgtaccgagc accacggcca
      661 tggctcctcc agccgatagg ccgatcgcat aaatcctaga ggggtcaatc cctccttccg
      721 ccaccacttc gccaacgatt ccatggagga tagccgcctc tcctacgtct cgacgttgct
      781 gatcttcgag gaaccaattc caacacaggt aggggttggc ctgacccgat tgctcgggaa
      841 atacggctac gaacgaagcc tcctccgcca gaaggtcaaa acccgtcagt ttggcaaacg
      901 aggcggcgga ttggttacat ccgtgcagaa ccacgacgag aggtttgtcc tgggggcctg
      961 caggtcggtg aaggagataa tcacgtttgc cttcgaggcc ggcgtacgtg cgacgctcga
     1021 gcgagggcag cggttcggcg gcatcggctt cgtcgaaagg ggcatccgcg ctttggtctg
     1081 gtggggagtc ctcgggtaaa tcctgggcag catcgagcgg tggttcggcg tcggacgagt
     1141 ctgtcacgcc agcgtcgggg gatgcagcgg gagatgggtc ctcggaatca caagcagagc
     1201 cgaataagcc aacgaggcag acaagcgcgg cttgatgtct catgggccag ttgtaccccg
     1261 gcaaggctca agggtcgtga gaaaacgacg ttaagaggcg gggattttgc gatgcaacat
     1321 gaatcaggag ccgacgggag tccaatcggc ggagcgtttc gcggtttcca tgaccgcatc
     1381 gcaccatgct ttatggacct tcattcccac gtcgaaggtt gtggtggaag ctttgtggaa
     1441 ggtatccagg gtggcgcgag ctttgtccca tccctcggct tgcaagtcca tcatggattt
     1501 ggtggaaccg gaaacggctt cgaggtggcg gtccaccatc ccggaccagg tcgaaaccat
     1561 ctcggtggga tcggagattt cccgagcatg ttccatgact tcactggaca attgttttgc
     1621 ctgttcgaga cgttcggaga gtttttccac gccttttttg ttccaactca tgagttcgtc
     1681 cgtcacgctc cccgccatct cttgttgttt gtcgatgagt tttttccaag cgccgatagc
     1741 ttgctgcaca tgcgaattga tggtttcggt catgataggg tcctccgtag ggggaatcat
     1801 tactgattcc tgttgttcat cagggttttg atgagcagga aggtaggacc gggcaagaat
     1861 atgtcaagag acaaaatgtt gcggcgcaac attttctttc atgtcttgtg tttttgctgg
     1921 atttcttttc gaagctcttc gatttcgcgt cgcaacgcgg ctacctcggg atgaaagtcc
     1981 gacggctccc cttcttcgac cggctcggcc gtgaccttcg gctcctgata gggcaccgaa
     2041 ctcgcaaaca tacgcgcgaa agcgctgatg gcttcgaagg gaaccgatgc gaaaggattg
     2101 aaggagctga ccacgctggc tccgcgtttc gcctgcaaat aaagcgcaag agccacggat
     2161 aagtaccgtc ccaaaaactc cgccaacgag gcatctccca aacggatgag ttgcaccaac
     2221 agactgacag gcagcaactt cgaggctc
//
LOCUS       DAOSOR010000766         2248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_48194_length_2248_cov_3.388965, whole genome shotgun sequence.
ACCESSION   DAOSOR010000766 DAOSOR010000000
VERSION     DAOSOR010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48194_length_2248_cov_3.388965"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..2032
                     /locus_tag="PK710_24660"
     CDS             332..2032
                     /locus_tag="PK710_24660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOT12997.1"
                     /translation="MRAMKGMGWELIGAVVCFLAACSSEKTSDDGASQPTDAGREVGK
                     DSGGGGSGGSGGHTGGDGNLIKPACTPSPQGGSTQVQKPTLFATLFDSWHEAWLGSAV
                     VADVDGDGTKEIVVARGSLVLGWHLDGQLVRKLETEGGRIWSSPVVADLVGASAGLEI
                     AAASRDSIYLWDAQGKLLPGFPFVWRDELRSLAAADIDGDGDLELVAVTTSPLSEHGQ
                     RDIIIAIHADGRVVDGFPPNTSGASGCDDACYVTGGFDQNLALGEVDGDGKADILAPQ
                     DNAYMSLHRGNGEAFDAAPIFKGRKKFPGIRFLHDYAQAQQGWAQDEATSNQAHFTNS
                     APALADIDGDGTHELIVLASVQNASQDDRLRGVALWVLKPDGTRPGPWVEPFHVPGYL
                     AGLWDFEGTNVVGATNQVTVADIDSQRPGPEFLFAGFDGKIHCVDASRNELWQATYTT
                     SDRVLTAGVVVADLSADGSPEIVFSTYSPDEGKSHLFVLDAAGNELHKTELPKRGAMP
                     VPTIADVDGDGQLEIVVSLKDGESGKQQVLVYTVAGSSDNCLLWPTARGNDLRSAFIP
                     "
BASE COUNT          432 a          531 c          741 g          544 t
ORIGIN      
        1 gtaccaattt atttccataa ataagcggaa cgtgcttcgt ggctgtagta ctagtgccac
       61 gaccggatat ggatgattcc tctcctggaa acgtcgagcc ctcccccaag cccgacaggc
      121 aaagcaaagg tgtcgaatct accgcttatc cttttgcctt tcccaacgga tggctgccgg
      181 tctttggtgt tgtaacatat tgtttttatt gttattaata ctcgttcggt tttttttcga
      241 ggggcgacgg ccagaattgc cggtctgtct tgcagttcag gcttatcgat atcaagctat
      301 tttcggatat tcctccacga aaggacgagc gatgcgagcc atgaaaggga tgggttggga
      361 attgatcggt gctgtcgtct gctttcttgc ggcttgctca tccgaaaaaa catccgatga
      421 tggagcgagc caaccgacgg atgcagggcg cgaggttggc aaggacagtg gtggtggtgg
      481 cagcggcggt agtgggggcc atacgggggg agacggcaat cttatcaaac cggcttgtac
      541 gccttcgccg cagggcgggt cgacgcaggt tcagaagccc accttgtttg ccactctttt
      601 cgattcttgg catgaggcgt ggttgggttc tgcggtggtg gcggatgtgg atggggatgg
      661 taccaaagag atagttgtcg cgcgcggcag cctggtgctt ggttggcatc tggatggtca
      721 gctcgtgcga aagctcgaga ccgaaggggg gcgcatttgg tcatctccgg tggtggcgga
      781 tctggtgggt gctagtgcgg ggctcgagat agccgcggcg tcgcgcgact cgatttatct
      841 atgggatgcg cagggcaagc tccttccggg atttcctttc gtatggcgtg atgagcttcg
      901 ttcccttgcg gcagcggata tcgatggcga tggggatctg gagcttgtgg ccgtgacgac
      961 ctcccctcta tctgaacatg gacagcgcga tatcatcatc gcgattcacg ccgatggccg
     1021 tgtggtggat ggttttccgc caaacacgtc cggggcttcg ggttgtgacg atgcttgtta
     1081 tgtgaccggg gggttcgatc agaatcttgc gctaggggag gtggatgggg atgggaaggc
     1141 ggatattctt gctccccagg acaatgcgta catgagtttg caccgtggaa atggcgaggc
     1201 attcgatgcg gcgcccattt tcaaaggtcg aaaaaaattc cctgggatcc gctttttgca
     1261 cgactatgct caagcacaac aaggctgggc acaggacgaa gccacctcga atcaagcgca
     1321 tttcaccaat agtgcgccgg cgctggcgga tatcgatggg gatgggaccc acgagcttat
     1381 cgtgctcgct tccgtgcaaa atgcttccca ggacgatcgg cttcgtgggg tggctttgtg
     1441 ggtattgaag ccggatggaa ctcgtcctgg gccatgggtt gagccttttc acgtgcctgg
     1501 gtatcttgcc ggattgtggg attttgaggg aacgaatgtg gtgggggcga ccaatcaggt
     1561 cacggtggcc gacattgact cccaacggcc ggggcctgag tttttgttcg cgggtttcga
     1621 tggaaaaatc cattgcgtcg atgcctcgcg aaacgagctt tggcaagcga cctacaccac
     1681 cagcgatcgt gtccttaccg ctggggtcgt tgtggcggat ctttcggccg acggttcccc
     1741 cgagatcgtt ttttccacct actcaccgga tgagggaaaa agccacttgt tcgtgctcga
     1801 tgcggcaggc aacgaactgc ataaaaccga acttcctaag cggggagcca tgcccgtacc
     1861 gaccatcgcg gacgtggatg gggatgggca gctcgaaatc gtggtcagtc tcaaggatgg
     1921 ggagagcggc aagcagcaag tactcgtcta tacggtggct ggatcgtcgg ataattgcct
     1981 gctttggccg acggcccggg gcaatgactt gcgaagcgcg ttcattccgt aagcaacgtt
     2041 gtgtttagcg gtggaggttc ggcgcaatgg atggcacgat tcatccaaca tttcctgaga
     2101 aggtgtgaag catgcggtgg atgacaagga tgatggtttc gtggctaggc gtatcgctgg
     2161 cgcttggcat ggtttgcggg tgcggaggag atgaggggga tacttcctct gggtcttcgg
     2221 gtacgtcggg aggctcggca ggctcggg
//
LOCUS       DAOSOR010000767         2228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_48747_length_2228_cov_4.619420, whole genome shotgun sequence.
ACCESSION   DAOSOR010000767 DAOSOR010000000
VERSION     DAOSOR010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48747_length_2228_cov_4.619420"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="PK710_24665"
     CDS             <1..700
                     /locus_tag="PK710_24665"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT12998.1"
                     /translation="FQVRVQAALALGASGSERAVGPLCGALDDETASVRAAAAAALAR
                     LRKPSGLSCLKARAGKETNGAVKTQIEKASARIEKDAEHQRNAKASRQPTRASRLYVA
                     VEATKTKHGRRKGEIELLVQATIRRLLLADAKVALAPSGQSPQDFDNAAKGNRVQGYL
                     LRPTVESVDYDGAKLSVALRVTLFSYPSMALQGEFFPKLSMSGTPQRDPEAEDELLQM
                     AAQKAVEKFLATTR"
     gene            758..901
                     /locus_tag="PK710_24670"
     CDS             758..901
                     /locus_tag="PK710_24670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT12999.1"
                     /translation="MTASPLVTISSNLDEVIKGIAVAARIDSRSASMVPMIRISSMTT
                     AGA"
     gene            1087..>2228
                     /locus_tag="PK710_24675"
     CDS             1087..>2228
                     /locus_tag="PK710_24675"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT13000.1"
                     /translation="MPSGPASRSQSENALAADPLLGTVLNDKYHVLAVVGSGGMGKIY
                     RAEQIPLGRPVALKVLHPAHTSTAKEGNFQQRFFLEASILSRLQHPNVVTVFDYGYIE
                     GTHPEMYFMAMEYLAGETLEHRIRRQGFLSPEELLPIVRQISRGLQDAHRQGVVHRDL
                     KPSNVMLVARSDGSMLVKIVDFGLVKVLGTDSDQITREGTFLGSARYMSPEQIVHGGV
                     DHRTDVYALGVLMFEALCGVPPFQREDLIQTLMAHVHEPIPEMASVAPNVHVPLAVEA
                     MVRRCMSKEPSERPPDMDSVLAEIDVCARALGFPVAPSDRYDVESGELMRGDHVVTRS
                     DALTRAEGRSPSLSITKSENAPLVFSPENPDTKTLARAKSSRKLLIV"
BASE COUNT          477 a          595 c          659 g          497 t
ORIGIN      
        1 ctttcaggtg agggtgcaag cggctttggc gcttggagct tccggttccg aaagagcggt
       61 ggggccgttg tgtggggctt tggacgatga aacggccagt gtgcgtgcgg cggccgcagc
      121 ggctttggcg cgtttacgta agccgtcggg gctatcgtgc ctcaaagccc gagcaggcaa
      181 agaaaccaat ggggcggtga agacgcagat cgaaaaggcg agcgcacgca tcgaaaagga
      241 tgccgagcac caaagaaacg cgaaggcctc ccggcagccc acgcgggcca gtcgattgta
      301 cgtagccgtg gaagccacca aaaccaagca tggtcgtcgc aaaggcgaga ttgagttgtt
      361 ggtgcaggca acgatccgca ggctgctgct cgcggatgcc aaagtggcgt tggcaccctc
      421 ggggcagagt ccgcaagact tcgacaacgc tgccaaggga aatcgcgtgc agggctacct
      481 gcttcgtccc accgtggaat cggtggatta cgatggtgcc aaactatccg tagccctgcg
      541 agtgacccta ttcagctatc catcgatggc attacaagga gagttttttc cgaagctatc
      601 gatgtcgggc acaccccaac gggatcccga agcagaagac gagcttcttc agatggcagc
      661 gcaaaaagcc gtggaaaagt ttttggcaac cacacgttga tgatctcatg acgggcgctg
      721 cgcactccat gcttttgttc ggagcagaga gggtacgatg acggcaagtc ctttggtgac
      781 catctcatcg aacctcgatg aggtgataaa agggatcgcc gttgccgcgc gcatcgattc
      841 ccgatccgcg tcgatggttc ccatgattcg tatctcttcg atgacgacag caggggcgta
      901 agggtcgccg acgtgaaacg ctctcgcgct gtgcgagcat cgccacactc aatgccgatc
      961 ggatgttggc cacgggagtc gcgaacttgc gttgtcattt ggacggtgac ttgtaatatg
     1021 gaaatatgct gttgttcgcc gtcccgattg agaactccat cgtcggcacc atgagggtgg
     1081 atatacatgc ctagcggtcc tgcctctcga tctcagtcgg aaaacgcact cgccgctgat
     1141 cctttgcttg gcacggttct caacgataag tatcacgttc ttgccgttgt cggcagcgga
     1201 ggaatgggaa agatctatcg agcggagcag attccgcttg ggcgacccgt ggcgctgaag
     1261 gttttgcatc cagcacacac gtccactgcg aaggaaggaa atttccagca gcgttttttc
     1321 ttggaagcga gcattttgtc tcgcctacag catcctaacg tcgtcaccgt ttttgattat
     1381 gggtacatag aaggaaccca tcccgaaatg tacttcatgg cgatggaata cctcgcgggt
     1441 gagacgctgg agcatcggat tcgtcgtcaa ggattcttat cgcccgagga gctattgccc
     1501 atcgtccgtc agatctcccg aggtttacag gatgcgcatc gccagggggt agtgcatcga
     1561 gatctcaagc catcgaacgt gatgctcgtg gcgcgatcgg atggttccat gctggtcaaa
     1621 atcgtcgatt ttggtctggt caaagtgctc ggaacggact cggatcagat cacgcgagaa
     1681 ggaacgtttc tcgggtcggc ccgctacatg tctcccgaac agatcgtgca tggcggggtc
     1741 gatcatcgca cggacgtcta cgcccttggt gttctcatgt tcgaggcttt gtgcggtgtg
     1801 cctccgtttc aacgtgagga cctcatccaa accctgatgg cgcatgtgca tgaaccgatt
     1861 ccggaaatgg cttccgtggc tccgaacgtt catgttcccc tcgctgtcga agcgatggtt
     1921 cgtcgttgta tgtccaagga accgagtgaa cgtccaccgg atatggatag tgttcttgcg
     1981 gagatcgatg tctgtgcccg agcccttgga tttcctgttg cgccttcgga ccgttatgat
     2041 gtcgaatccg gagaactcat gcgcggcgat cacgtcgtca cccgttccga cgcattgacg
     2101 cgagcggagg ggcgttctcc ttccctgagc atcacgaaat ccgaaaatgc tcctcttgta
     2161 ttttctcccg aaaacccgga tacgaaaaca ctagcccgag ccaagtccag caggaagctg
     2221 ttgatcgt
//
LOCUS       DAOSOR010000768         2210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_49302_length_2210_cov_3.915081, whole genome shotgun sequence.
ACCESSION   DAOSOR010000768 DAOSOR010000000
VERSION     DAOSOR010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49302_length_2210_cov_3.915081"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="PK710_24680"
     CDS             <1..1129
                     /locus_tag="PK710_24680"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOT13001.1"
                     /translation="DGTVLLAGSEDKTVRMWDAATGKLLNQWRGHRGPVVALAFAPDS
                     SVAVTASEDASLAIWDIRRRGLRTWLKGHESAVRAVAVSPDGVRIASGGDDKTLRIWE
                     TSTAKLRDTIFAHGDSIRAVAFCPDGSLIATSGRDQTVKLWDSGDGGLRATYRGHAGS
                     VDAVAFSPNGQFLAAAGESVLMHRRSDSHALVLRTFAVDGEEVGLAHTLDGVFEGDPR
                     ALRRVLYRVGDDVATSELLSAEQLSESFFHDGLGVAFSEGRPISVPMESEHGIGLPPS
                     VEFVDPLPKQVASDTVEVSVQAKSRGGGIAAIRLYVNGELIKDSSAQSEDKRSVKGLS
                     EIVVYRVPLVVGDNVLEAEAVNIQGRVKSPRVSVTILRKES"
     gene            1161..2081
                     /locus_tag="PK710_24685"
     CDS             1161..2081
                     /locus_tag="PK710_24685"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOT13002.1"
                     /translation="MIHVFGAVLVDAIACHDAFTPGTSCIATVRTAMGGVGFNIFRAL
                     GSAPRRFITALGDGPFSHYAKEALEAYADQIMVRVMPNRDVGMYLALMQRGRLLYGVT
                     DASTFEEAMDPGWLETVIRAVCPQDIVVIDANLVPDTIAYLVDAVARRGAWIVFEPVS
                     VDKAARSRKALHDVFLITPTEQELEALVGTQEPREKQVFEWMRQRRVEHLVVTMGAEG
                     LQWYDTTHTIRLRPLRTIDVRDTTGAGDQLMGTLLSEVHAARMTLPEGVSSSSARETV
                     GKSMEEKLRRAMDDVEAMLLERLSSLEQCC"
BASE COUNT          440 a          512 c          726 g          532 t
ORIGIN      
        1 tgatgggacg gttttgcttg cgggaagtga ggataagacc gttcgcatgt gggatgccgc
       61 cacgggaaag ctattgaacc aatggcgggg ccatcgagga ccggtggtgg cccttgcgtt
      121 tgcgccagat tcctccgtgg cggtgacggc ctcggaggat gcgtctctcg ccatttggga
      181 tattcgacga cgcggattgc gaacatggtt gaaagggcat gagtcggcgg tgcgtgccgt
      241 ggcggtaagt cccgatggtg tgagaattgc ttctggtgga gatgacaaaa cacttcgaat
      301 ttgggaaacg agcactgcca agttgcgaga taccatcttc gcgcacggtg attcgattcg
      361 agcggtggcc ttttgccccg atggaagcct gattgccacg tcggggcggg atcaaacggt
      421 caagttgtgg gactcaggag atggtgggtt gcgggcaacc tatcgagggc atgccggttc
      481 cgtcgatgcg gtggctttca gtcccaatgg tcaattcctt gccgcggctg gagagtccgt
      541 actcatgcat cgccgatccg acagtcacgc ccttgtgctg cgcacgtttg ctgtcgatgg
      601 cgaagaagtg ggtcttgcgc ataccttgga tggtgttttt gaaggggatc ctcgggcttt
      661 aagacgagtg ttgtatcggg tgggcgacga tgttgcgacg tcggagttgc tcagtgcaga
      721 gcaactcagt gagtctttct tccacgacgg gttgggagtt gccttttcgg aggggcgtcc
      781 catctcggtt ccgatggagt ccgagcatgg gattggtctt cctccatcgg tggagtttgt
      841 cgatccgctg cccaagcagg tggcctcgga tacggtcgag gttagcgttc aggcgaaaag
      901 tcgcggaggc ggtattgcgg ccatacgatt gtacgtcaat ggtgagctta ttaaggatag
      961 ttcggcgcaa tcagaggata agcggagcgt gaaagggctt tccgaaattg ttgtctatcg
     1021 tgtccccttg gtcgtgggcg acaacgtact cgaagcggaa gcggtcaata ttcaggggcg
     1081 tgtcaagagc cctcgtgtta gcgtgacgat cctgagaaaa gaaagctgag caacaacggc
     1141 tttggttgtc tggactcctc atgattcatg tttttggtgc cgtattggtt gacgccattg
     1201 cgtgtcacga tgcgtttacg cccgggacgt cttgtatcgc taccgtccga acggcgatgg
     1261 gaggggtggg cttcaacatt tttcgagcgc ttggttctgc gccccggcga ttcatcacgg
     1321 cattgggcga tggacctttc tcccactatg caaaagaagc gctcgaagct tatgccgatc
     1381 aaatcatggt tcgtgtcatg ccgaaccggg acgtgggtat gtatcttgcc ctcatgcaaa
     1441 gagggcgttt gctttatggc gtcacggatg catccacatt cgaggaggct atggatccgg
     1501 gctggctgga gacagtgata cgagccgtat gcccgcaaga tatcgtggtg atcgatgcca
     1561 atctcgtacc ggacaccatt gcttatcttg tggatgcggt ggcgcggcgc ggagcctgga
     1621 tcgtttttga gcccgtttct gtagacaaag ccgcgcgttc gcgaaaggca ttgcatgatg
     1681 tgtttttgat cacacccacc gaacaagaat tggaagcgct cgtgggaacc caggaaccca
     1741 gggaaaaaca agtgttcgag tggatgcgac aacgacgtgt cgagcatctt gtggtcacga
     1801 tgggagccga aggcctgcag tggtatgaca cgacacacac aatacgattg agaccgttgc
     1861 gcacgatcga tgtgcgagat acgacgggag cgggtgacca attgatggga acgctccttt
     1921 cggaggtaca tgccgcgcgt atgacgcttc cggagggggt atcgagttca tcggcgcgtg
     1981 aaaccgttgg aaagtccatg gaagaaaaac tgcggcgggc gatggacgat gtggaggcca
     2041 tgctgctgga acggttgtca tctttggagc agtgttgcta agggagttct tgcttgcttg
     2101 gtggcgcgtc tttctggata ggcgcatcgc cccttgatat tcatcatggc ttcgcgaatt
     2161 cgtttcgggt ggttgagtgt gaagcagaaa ctgcaccatg tgccgtggcc
//
LOCUS       DAOSOR010000769         2202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_49553_length_2202_cov_4.035864, whole genome shotgun sequence.
ACCESSION   DAOSOR010000769 DAOSOR010000000
VERSION     DAOSOR010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49553_length_2202_cov_4.035864"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            415..>2202
                     /locus_tag="PK710_24690"
     CDS             415..>2202
                     /locus_tag="PK710_24690"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT13003.1"
                     /translation="MGQRGPSRAIILAAGMGSRLVAEEAFPKPLMPVAGVPLLVRVLR
                     NLQMEGIREAVIITGYRGEQIEQRLLAEPSLVLRLRFVRNHDYQLKNGVSLLKAARYV
                     DRECILSMADHLYSPELVRRIRQFSLPEGHCVLGVDYDVDRCFDIDDATKVRVSGGRI
                     VDIDKSLEQIDCIDTGVFRIGPDFIRELERVREATGDCSLSDGVRAMAQRGKFLVCDI
                     GDARWIDVDTPQALQRAEAMIRVFGDGLGDEPRSSASPVAPEAMEYFAPSWVRAVQPY
                     NEEHFDVAASRDGVARMMSNESPYEPSERVIRAVVEAARHGNVYPTGARGLRERLAGR
                     EGFAEDFVTLGSGSTELIDLIIRTFVAPGEEVLLSVPTFSMYEARTRAVGGVPVMVPI
                     TDDNAFDVHGIIASITERTKVIFLCAPNNPTGARLDEASVRRILGLGLPTVIDEAYYE
                     FADNPRTLSHVLTEYPNAIVLRTLSKAFGLAGFRLGYAFSHPVVTRLIGRLKLPWNIN
                     GVTIAAASAVLDDMAEFDDRMRRLRSGRDYLARELAMVPGLRVLTTDGNFVLVDTHHT
                     GIEPESLVQAMFAEGVLIRSLAVHHAEHSY"
BASE COUNT          419 a          493 c          762 g          528 t
ORIGIN      
        1 aggggggtgg acgtcggtga tctgagttgg atcgaggtag atacggcggg ggatttgtct
       61 cgggcggaag cgatggtgcg cgctgggctg ttgtaggttt gcaccggggg gcgtgtaacg
      121 acggtagcag tggatgctgg cactttcgtt gcttcaaagc gatgcacgtt tggcggttac
      181 accacactgt gaacatgcat tcacaatttc gtcgataaga aagcttggtt gagaacagag
      241 gcggagtcca gggtgtgatt tccgtgcaac agggtgtggt gtaccgacaa cacaaaggga
      301 tggtaaccgg ctgagacaca agcggaattg gttgcaaaag attacccact tgggcgagcg
      361 gcgtgctaaa ggccgcgaga ttcttgtgag cttggttagg aaaggttaat gaacatgggt
      421 cagcgaggac cgtcgagggc catcattctt gccgcgggta tgggatcgcg tcttgtagcg
      481 gaagaggcgt ttcccaagcc gctcatgccg gtcgccgggg tgcctttgct cgttcgcgtt
      541 cttcgcaatt tgcagatgga gggcatccgc gaagcggtca tcatcacggg gtatcggggc
      601 gagcagatag agcagcgact gctggcggag ccgagcctgg tgttgcgtct gcgcttcgtt
      661 cgcaaccacg actatcagct caaaaacggt gtgtccttgc taaaagccgc tcgatacgtg
      721 gatcgggaat gcatcctatc gatggcggat cacctgtatt cgccggagtt ggtgcgtcgt
      781 attcgacaat tttccttgcc tgaggggcat tgcgtgctgg gtgtcgatta cgatgtggac
      841 cggtgtttcg atattgacga cgccacgaag gtgagggtgt cgggggggcg gattgtcgat
      901 attgacaaga gcttggagca gattgactgc attgacacgg gggtgtttcg cattggtccg
      961 gacttcattc gcgaactgga gcgggtgcga gaagccaccg gtgattgttc gttatcggac
     1021 ggagttcggg cgatggcgca gcgcggcaaa ttcctggttt gcgatattgg cgatgcgcgc
     1081 tggatcgatg tggatacgcc gcaggctttg cagcgtgcgg aagcgatgat tcgggtcttt
     1141 ggtgatggct tgggtgatga gccccggtcg agcgcgtcgc cggtagcacc cgaggccatg
     1201 gaatatttcg ctccgtcgtg ggtgcgggcg gtacaacctt ataatgagga gcacttcgat
     1261 gtggcggcgt cgcgcgacgg ggtagcgcgg atgatgtcca acgagagccc ttatgagccg
     1321 agtgagcgtg tcatccgtgc ggtggtggaa gcagctcgcc atggtaacgt ttatccaacc
     1381 ggcgctcgcg gactgcggga gcgcttggcg gggagggaag gattcgcgga ggatttcgtc
     1441 accttggggt cggggtcgac ggagttgatt gacttgatca ttcgtacttt cgtcgcgccg
     1501 ggagaagagg ttctgttgag tgtcccgact ttctccatgt atgaagcgcg aacccgcgcg
     1561 gtcggagggg taccggtgat ggtgccgatt accgatgata acgctttcga tgtgcatggg
     1621 atcattgcga gtatcacgga gcgaaccaaa gttatttttc tatgtgcgcc gaacaatcca
     1681 acgggagcgc ggctcgatga agcatcggtg cgtcgaattc ttgggctcgg gttacccacg
     1741 gtgattgacg aggcttatta tgagtttgcg gataatccgc ggactttgtc tcatgtactg
     1801 acggaatatc cgaatgccat tgtgcttcga acgctttcga aggcgttcgg gttggcggga
     1861 ttccgtttgg ggtatgcgtt ttcccatccg gtcgtgacgc ggctcattgg tcgattgaaa
     1921 ctgccgtgga acatcaatgg ggtcacgatt gctgcggcgt cggcggtgtt ggatgacatg
     1981 gcggaattcg atgatcgcat gcgacgactt cgcagtgggc gggactatct cgctcgcgaa
     2041 ttggccatgg tgccagggct aagggttttg acgaccgatg gtaacttcgt gttggtggat
     2101 acccatcaca cgggtatcga accggagtcg cttgtacaag cgatgtttgc agagggtgta
     2161 ttgatccgct cgctggctgt tcatcatgcg gagcacagtt ac
//
LOCUS       DAOSOR010000770         2201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_49585_length_2201_cov_5.055918, whole genome shotgun sequence.
ACCESSION   DAOSOR010000770 DAOSOR010000000
VERSION     DAOSOR010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49585_length_2201_cov_5.055918"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PK710_24695"
     CDS             complement(<1..313)
                     /locus_tag="PK710_24695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13004.1"
                     /translation="MLKPSPRRNIFVGILLLILAVGVAAYDYVQPATDGASWFRRYLL
                     VIDGGVLLLGVAAFVRGVWVFASGQQGGPRESESRTMLFFRRNYRKLLVSATVLTGVV
                     FG"
     gene            complement(317..2029)
                     /gene="lysS"
                     /locus_tag="PK710_24700"
     CDS             complement(317..2029)
                     /gene="lysS"
                     /locus_tag="PK710_24700"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOT13005.1"
                     /translation="MTKQEGNQKDEDRIPAGSSESALIATRKAKAERMRQRGQDPFTN
                     DIEPERITPLGDVRARFASALGEQGTYDSEAVARQVQDSAWGQSVTVAGRVIASRSFG
                     KVHFLRIRDWSGEIQLLCRKNVLGDAFARLEDIDVADHVTATGGVMVTKTGELSLEVN
                     DLRLLTKAYRPLPDKWAGLSDVELRYRRRYVDLVSNEQARAVFRARSVIVRALRTYLD
                     DRGFLEVETPTMNTLVGGATAKPFVTHHNALGLELFMRIAPELHLKRLVVGGFERVYE
                     VARCYRNEGLSTRHNPEFTMVEFYMAYATYETLMDMTEDMLRHVDEALQRAMPQEHAQ
                     WKNSRPFTLDEPFHRKTMLELLRMRLGAEARALAEQTEATVAALMSAGLDDGALQDGP
                     KLRGVVEKACDVGGLDKVVVKQLRGCESHGELVFVLYELIVEPELTRLFVKGDKSVPV
                     FVTDHPVEVSPLARRQTGPNGNPDLVDRFELFVHGRELCNAFSELNDPEDQAQRFLDQ
                     VRKKERGAEETMDYDEDYIRALEHGLPPTAGFGMGVDRLTMALTAQDSIREVILFPLM
                     RPEH"
BASE COUNT          477 a          751 c          539 g          434 t
ORIGIN      
        1 caccaaatac gacacccgtc agcaccgtcg cggaaaccag aagcttgcga taattgcgcc
       61 tgaaaaacag catcgtccgc gactcggact cgcgcggtcc cccctgctgc cccgaggcga
      121 aaacccatac gccccgaacg aacgcggcca ccccaagcaa caacaccccc ccatcgatca
      181 cgagcagata acgacgaaac cacgaggctc cgtcagtcgc gggttgcaca taatcatacg
      241 cggccacccc caccgccaga atgagcagca ggatgccaac gaaaatgttg cgacgtggac
      301 tgggtttcaa catgggtcag tgctcgggtc gcatgagcgg aaaaagaatc acttctcgaa
      361 tcgaatcctg tgcggtcaaa gccatcgtga gacgatcgac acccatgccg aagcccgccg
      421 tgggaggcag gccgtgctcc aacgcacgaa tatagtcctc atcatagtcc atcgtttctt
      481 cggctccacg ctcctttttc ctcacttgat cgagaaaacg ttgcgcctga tcctccggat
      541 cattcaactc gctgaaggca ttgcataatt ccctgccatg cacgaataac tcaaaccgat
      601 ccaccaggtc aggattgccg ttgggccctg tttgacgacg cgccagcgga gatacctcca
      661 ccggatgatc cgtgacgaaa acgggcacgc tcttgtctcc cttgacgaat aacctcgtca
      721 attccggctc gacaatcagt tcgtaaagca cgaacacaag ctcaccatgg ctttcacatc
      781 cccgaagttg cttgacgacc accttatcca agcctccgac atcgcacgcc ttttccacca
      841 cccctcgtag ctttggcccg tcttgcagcg ccccgtcgtc cagcccagcg ctcatcaacg
      901 ccgccaccgt tgcctcggtt tgctcggcca acgctcgcgc ctcggctcca agacgcatcc
      961 tcaatagctc cagcatcgtt tttcgatgaa atggctcatc cagcgtaaac gggcgactgt
     1021 tcttccactg cgcatgctct tgcggcatcg cccgctgcaa tgcctcatcc acgtgacgaa
     1081 gcatgtcttc cgtcatgtcc atcaacgtct catacgttgc atacgccatg tagaactcaa
     1141 ccatcgtgaa ctccgggttg tgacgtgtcg acaacccttc gttgcgataa caccgcgcca
     1201 cctcatacac ccgctcgaat ccgcccacga ccagccgctt caagtgcaac tcgggggcga
     1261 tacgcatgaa taattcgagc ccaagggcgt tgtgatgggt cacgaaaggc ttggctgtcg
     1321 caccccccac caacgtattc atcgtcggtg tctccacctc gagaaatccc cggtcgtcca
     1381 agtacgtccg caacgcccga acgatcaccg agcgcgctcg aaataccgcc cgcgcctgct
     1441 cgttggatac gagatccaca tagcggcgac gataccggag ttcgacgtcg gaaagccccg
     1501 cccatttgtc cggcagcggg cgatacgcct tggtcaaaag gcgcaaatca ttgacttcta
     1561 acgacaattc cccggttttc gtgaccatca ccccgccggt agccgtcacg tggtcggcca
     1621 cgtcgatgtc ctcgagtcga gcaaaagcgt caccaagcac gttttttcgg caaaggagct
     1681 gaatctcccc agaccagtcc cgaatacgta ggaaatgcac ttttccaaac gaacgggaag
     1741 cgatcacgcg cccggccacc gtcacgctct gcccccatgc cgaatcctgc acttgccgcg
     1801 ctaccgcttc cgaatcgtag gtgccctgct caccaagcgc cgaggcaaag cgtgcccgaa
     1861 catcccccaa cggcgtgatc cgttcgggtt cgatatcgtt ggtgaaaggg tcctgtcccc
     1921 gctggcgcat tcgctcggcc ttggctttgc gggtggctat caacgcggac tcggacgagc
     1981 ccgccggaat ccggtcctcg tccttttggt tgccttcttg cttcgtcatg ccgtctgggc
     2041 ctgtagcgtg gtaactacgg attgtccacg gaggcgttgc gtgtgactgc catcgaatat
     2101 gaaacctgga tgctcctggc cctgcaacaa gccgacctcg ccgcagccca tggagatgtg
     2161 cccgtcggat gcgtcatcgc cgaccctcac ggaaccatca t
//
LOCUS       DAOSOR010000771         2199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_49653_length_2199_cov_3.763993, whole genome shotgun sequence.
ACCESSION   DAOSOR010000771 DAOSOR010000000
VERSION     DAOSOR010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49653_length_2199_cov_3.763993"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="PK710_24705"
     CDS             complement(<1..800)
                     /locus_tag="PK710_24705"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HOT13006.1"
                     /translation="MTLPFEFLNPAGLWMLSALAPLIVLYILKIKRQRLRVPSTWLWV
                     SAQRDLLAKSPFQRLTPQIPLFLQILIILLLSLALARPSTRSEAISGDHLALVIDTSA
                     SMSALDESGKTRLELAKASALRVIDALGPSSDAMVLEAGHDARIASPMDRDRRRLQAA
                     IEMLQTSDTEGDLGTAVALAVDRLRQLGGQVRVIVFTDGALAHPDSLSSVSIPMQLVK
                     VGSTIDNAAIVRVDVRRGRDAKADTDQVQVFAMLANYGTRARESFVTLR"
     gene            complement(1061..1696)
                     /locus_tag="PK710_24710"
     CDS             complement(1061..1696)
                     /locus_tag="PK710_24710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13007.1"
                     /translation="MKRLIPFFISCLLVACASDDEGYDDKNPNPSDAGKDVAADTVAT
                     DSNTKDTATNDVVDDKVTQPDVIPDAIPEATPDTNPEDTGVPDVGPDVEFDAPADVKP
                     DIGPVSSCSELSTNECFSNKDCVDTLRCSNAGTETDPVPCCVSGPRGTGKAGTPCKSE
                     NDCESGVCIEGTSKQLCSKACSSNDDCPDGMKDCRYIWGSGSSDDWCFPTN"
     gene            1870..>2199
                     /locus_tag="PK710_24715"
     CDS             1870..>2199
                     /locus_tag="PK710_24715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13008.1"
                     /translation="MNVRLGTVFMTLLLSVGLLMGPLGCGGSSEADLPPGTGFDAADA
                     PNDAPDAASDSSGDSSPDVALDVAPDVAPDVVPDVAPDVAPDVVPDVAPDVAPDVAPD
                     VVPDVVPD"
BASE COUNT          471 a          646 c          636 g          446 t
ORIGIN      
        1 cgaagcgtga cgaacgactc gcgcgcccgc gttccataat tcgcaagcat cgcaaatact
       61 tgcacctggt ccgtatccgc ttttgcatcg cgaccccgtc gtacatccac tcgcacgatg
      121 gccgcgttgt cgatcgtcga ccccactttg acgagctgca tcggaatgct aacggatgac
      181 agcgaatctg gatgcgccaa cgcaccatcc gtgaatacga tgacacgaac ttgaccgcca
      241 agctgacgga gacgatccac ggccaacgcc accgcggttc ccaaatcgcc ctccgtatcc
      301 gaggtttgaa gcatttcgat ggcagcttgc agacgacgtc gatcgcggtc catgggggag
      361 gctatgcgcg catcgtgtcc tgcctccaac accatggcgt cactgctcgg acctagcgcg
      421 tcaatcacgc gcagggcgga agctttcgcc aactccagcc gcgttttgcc agactcatcg
      481 agcgccgaca tcgaagcgga cgtgtcgatg acgagagcaa gatgatcgcc agaaatggcc
      541 tcggaacgag tcgaaggtcg cgcaagggcc aaagacagca ggaggatgat caggatctga
      601 aggaacaaag ggatttgggg agtgagccgc tgaaagggag acttggcgag gagatcgcgt
      661 tgagccgaaa cccacaacca cgtcgaaggt acccgaagcc gttggcgttt gattttcaat
      721 atgtacagca caatcagcgg cgccagggcg ctgagcatcc aaaggccagc cggattcaag
      781 aactcgaacg gcaacgtcat gcgggtcgaa acgctttcgc cggaatcatg accggccacg
      841 ggagaatagc aaccgttagc cgtgggaact aggggctctc cactctgact ccaggaatcg
      901 aagccgtccg tgcgggataa tgagatttat gctttaaatt caagtggtta cagtgagccg
      961 gacaaagatg gggcgtcatg accccgtcct tttctaccgg gggccgcgtt caggaccaac
     1021 accgggtccg gacaatcgtg tgacgacggg ggaagcagac tcaattcgtc gggaaacacc
     1081 aatcgtcgct gcttccactg ccccagatgt acctgcaatc cttcatccca tccggacagt
     1141 catcgttgga agaacaagcc ttggagcata gctgcttgct tgtgccttcg atgcaaaccc
     1201 cgctttcgca atcgttttcc gacttgcaag gagtccccgc ctttcccgtg ccccgagggc
     1261 cgctcacgca acatggtaca ggatcggttt cggttcccgc attcgagcat cgtagcgtgt
     1321 cgacgcagtc cttgttcgaa aaacactcat tggtgctcag ctcgctgcaa ctcgaaaccg
     1381 ggccaatatc gggcttcacg tcagcgggag catcgaactc gacgtctgga cccacatctg
     1441 gaacgcccgt atcctcagga ttggtgtccg gcgtggcctc cgggatggcg tcagggataa
     1501 cgtccggttg tgtgaccttg tcatccacga catcgttcgt cgctgtgtct ttcgtattcg
     1561 agtcggtcgc gacggtatcc gccgcaacgt cctttcccgc gtccgaagga ttcggattct
     1621 tgtcgtcgta accctcatcg tcagaggcac aagccacgag caagcacgaa atgaaaaaag
     1681 gaataagtct cttcatcccc tgagaattgc tgctatcttc cgaaaaatca agggcttgtt
     1741 cgttggccgg gggggcaagg gagtgccttt tttcgaagat gccccgaacg accgctggct
     1801 tcgtttcgac agaggtgtac aatcatctcc aacctcggtc gtaaaaccaa caccataagg
     1861 ggaaagatcg tgaacgtacg cttgggcacc gtcttcatga ccttgttgct gtcggttgga
     1921 ttgctcatgg gtccgttggg ctgtggggga agttcggaag ccgacttgcc tccggggacg
     1981 ggcttcgatg ccgcggatgc gcccaacgac gcacccgacg ctgcctccga ttcgagcgga
     2041 gattcatcgc cagacgtggc actcgatgtc gcgcccgatg tcgcgccgga tgtggtgccc
     2101 gatgtcgcgc ccgatgtcgc gccggatgtc gtgccagatg tcgcgcccga tgtcgcgccg
     2161 gatgtcgcgc cggatgtcgt gcccgatgtc gtgccggat
//
LOCUS       DAOSOR010000772         2188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_50006_length_2188_cov_4.296296, whole genome shotgun sequence.
ACCESSION   DAOSOR010000772 DAOSOR010000000
VERSION     DAOSOR010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50006_length_2188_cov_4.296296"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="PK710_24720"
     CDS             complement(<1..1230)
                     /locus_tag="PK710_24720"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOT13009.1"
                     /translation="MRFVSSLTSISLVVLPLLTGSTAFAATLQVGPGKTFGAPCDAIK
                     AASDGDIIEIDASGTYKGDVCAVTRNNLTLRGVGGRATIDANGAHHGGKAIWVITGAN
                     VVVENIEFLGAKVPDRNGAGIRQEGKNLTVRGCYFHDNENGILTSADSESEILIESTE
                     FAHNGYGDGQSHNLYIGHVKRLTFQFNYSHHAKIGHLLKTRAAENYILYNRLTGESGN
                     QSYEIDVPNGGLTYIIGNLVHQGQNTDNSSMLSYRREGAHANNPSQQLFVVHNSFVSD
                     RNTGIFLNVDPGVSPPAVVRNNIFMGKGTIVTQTNADVSGSFEGDPGFVDKDGFDYRL
                     SPGSPAENAGVAPGTGAGFDLTPTHHYVHPLGAEGRVTVGAIDIGAYEIGESAGSGGS
                     AGAGGGAGAGGDGGSAGS"
     gene            complement(1493..>2188)
                     /locus_tag="PK710_24725"
     CDS             complement(1493..>2188)
                     /locus_tag="PK710_24725"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOT13010.1"
                     /translation="KFSGTSMAAPHVSAAAALLMSLGVTEPGRVQAMLEASAEVPEGE
                     IKGGPRFGQGIVQVGKAVRATVGVQAATRLALVFGLTAYVGRRIRRKGGFSRFWRPGF
                     WLMALAFGPGLLAWAPIIVSRVSLPIDLLARPVPDWDLLLGVSVHRWLPLAHVLIPFS
                     LSAVGFGIRWARPFIAGIAVGIAAYLGSVPLLELTTGNLISRLLLATWALINIAGCLW
                     LAVLHLDEREVSS"
BASE COUNT          496 a          657 c          586 g          449 t
ORIGIN      
        1 gcttccagcc gagcccccgt cgccccccgc tcccgcacct cctccagcgc cagcacttcc
       61 ccccgaacct gcgctttccc cgatttcata cgcgccgata tcgatagcgc caaccgttac
      121 ccgcccctcc gccccgaggg gatgcacata atgatgcgtc ggggttaggt cgaatcccgc
      181 tcccgtgccg ggagcaaccc ccgcgttttc tgccggcgaa cccggtgaca atcggtagtc
      241 gaagccatcc ttgtccacaa aaccagggtc gccttcaaaa ctgccggaaa cgtccgcatt
      301 ggtttgcgtg acgatcgtgc ccttgcccat gaaaatattg tttcgcacca cggcgggtgg
      361 ggaaacacct gggtcgacat tgagaaatat tccggtattt cggtcactta cgaatgaatt
      421 atggacgacg aagagttgtt gactcgggtt attggcgtgg gcgccttccc ggcgatacga
      481 aagcatggaa ctattgtcgg tgttttgccc ttgatgcacg aggttgccga tgatgtaagt
      541 caaaccaccg ttgggcacgt cgatttcgta gctctgattg ccggattcac cggtgagacg
      601 attgtacagg atgtagtttt ccgcggcccg ggtcttgagc aagtgcccga tcttggcgtg
      661 gtgggaataa ttgaattgaa acgtaagccg tttcacgtgc ccgatataaa ggttgtggct
      721 ttgaccatca ccgtagccgt tatgggcaaa ctccgtggat tcgatgagaa tttcgctttc
      781 ggaatcggca ctcgtcagga tgccgttttc gttgtcatga aagtagcacc ctcgaacggt
      841 caggtttttt ccttcttgac gaatgccggc cccattgcga tccggaactt ttgcgcctaa
      901 aaactctatg ttttcgacga ccacgttggc tccggtgatg acccaaatgg cctttccccc
      961 gtgatgagcc ccgttggcgt cgatggtcgc gcgacctccc acgccacgta gggttaggtt
     1021 atttcgagtt accgcgcata cgtcgccctt gtaggtgccg gaagcatcga tttcgatgat
     1081 gtctccgtcg gaggcagctt tgatggcgtc acaaggcgca ccgaaagttt ttccgggccc
     1141 tacctggagc gtggcagcga aagcggtcga acccgtgagc aagggaagca caaccaagga
     1201 aatactggtc agggatgata cgaagcgcat gcaattacct tttgtggaaa tagtaagtct
     1261 ctagcctacg gcgccatcaa gcgggtttca tgccatttcc acgaaatttc cgacagggct
     1321 gaagtcccgc tcttcggcgt tcgcccatgc gccatcggtg tcgccatccc gtcatcggcc
     1381 cgtaccaaat tggatccaag ttgtcgcacc aaaggccggt atttcgtgat gctaccgagg
     1441 aaccgggacg aaccggtcca ccgaacaccg gcagcaactc gtgattattt cactacgacg
     1501 atacttctcg ttcatcgaga tgcaacaccg cgagccaaag gcatccagcg atgttaatca
     1561 aggcccaagt agctaataat aaacgagaaa tcaggtttcc cgtcgtgagt tccaaaaggg
     1621 gaacgctccc caaataggca gcaattccca cggcaattcc cgcgatgaaa ggacgagccc
     1681 agcgaatccc aaaaccaacc gcggatagcg aaaaggggat cagaacatgc gccaacggaa
     1741 gccaacgatg caccgatacg ccaaggagca agtcccagtc gggcacagga cgcgccaata
     1801 agtcgatagg cagggaaaca cgggacacga taataggcgc ccacgcgagc agccctggcc
     1861 cgaacgccag ggccatcagc cagaaccccg gtcgccagaa ccgcgaaaag cccccttttc
     1921 gtcgaatacg ccgccccaca tacgccgtca ggccaaacac cagagccagg cgagtggcag
     1981 cctgtacccc cacggtggct cgaacggctt tgcccacctg aacgattcct tgtccaaagc
     2041 gcggtccgcc tttgatttcc ccttcgggaa cttccgcgct agcttcgagc atcgcttgca
     2101 cgcgtcccgg ttcggtcacg ccaaggctca tcagcaacgc ggcggcagcc gatacatggg
     2161 gcgccgccat agaagtaccg gaaaactt
//
LOCUS       DAOSOR010000773         2157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_51036_length_2157_cov_5.090866, whole genome shotgun sequence.
ACCESSION   DAOSOR010000773 DAOSOR010000000
VERSION     DAOSOR010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51036_length_2157_cov_5.090866"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PK710_24730"
     CDS             <1..209
                     /locus_tag="PK710_24730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13011.1"
                     /translation="CVITPSSSILSFSIFGPLLRPDLFGEFMQLPRGQSELLRWFGTE
                     PNRTVNPKLGTIVRLCEHAYKNPG"
     gene            complement(216..479)
                     /locus_tag="PK710_24735"
     CDS             complement(216..479)
                     /locus_tag="PK710_24735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13012.1"
                     /translation="MTALREQRSRRWLAGGAVGLLVGIGLSFLGSGPVGGWIATAAAV
                     VLVMGIHTFGRLGPDLPPRSDSKEPKSQKKSRNKRKAIRRSDP"
     gene            complement(476..1000)
                     /locus_tag="PK710_24740"
     CDS             complement(476..1000)
                     /locus_tag="PK710_24740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13013.1"
                     /translation="MKSQLVAQRAMLSLGASILVYLSSVGCGPPEARTPNPLRSVDEG
                     RALKLIARAFSAEGIQPTAPRWVSLSGNTKVRLDVGAEGRKLGVVYLTASDVHELGDN
                     PLAKRSSTGSELIVRAGEGPDDGLRAVILYASDYVYDDNVGDDREASAITAENKLDRD
                     VRDFIVIARKNHWP"
     gene            1138..1764
                     /locus_tag="PK710_24745"
     CDS             1138..1764
                     /locus_tag="PK710_24745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13014.1"
                     /translation="MSYQGLLYCILISTLGGCASQRISDPRSAAEAYAQAASRGDGDM
                     IYDMLDEQAQRSMTRAEVRKLVANQREELAEQAKAIRSPQARVRSTATIRFADGEDAI
                     LDLSENGQFLIVSADALPAGARTPVQALDQLRHVLARRSYPGLLRVLSTSTRSAMEND
                     LRSLVEGLENPEELDVQQSGETATVRIPGGHWIVLRREGATWRIDNID"
     gene            1740..>2157
                     /locus_tag="PK710_24750"
     CDS             1740..>2157
                     /locus_tag="PK710_24750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOT13015.1"
                     /translation="MAYRQHRLTWFWVLACLLFVSSIPGKVRAMGDEGAFHPRVLIVG
                     EARFEGARTTAPARWSWELTRRTSAPARLAPTTVRADSPTLLEEPFVWWLGSTDVGAL
                     SSREITGLRAFFALGGVLFVDDSDPTTGVFERSAKRE"
BASE COUNT          416 a          597 c          648 g          496 t
ORIGIN      
        1 tctgtgtgat cacaccttcc agttcgatct tgtccttttc gattttcggg ccactccttc
       61 gacccgattt atttggtgaa ttcatgcagc ttcctcgagg ccaatcggaa ttgctgaggt
      121 ggtttggcac ggagccaaac cggacggtca atccgaagtt gggcaccata gtccgcttgt
      181 gcgaacatgc ctataagaat cctggatgaa agtgatcatg gatcgcttct tcggattgct
      241 tttcgtttgt ttcgggattt tttctgagat ttcggctctt tcgaatccga tcgcggtggc
      301 agatcggggc ccaagcgtcc gaaggtatgg attcccatga ccaaaacgac ggcagcggcg
      361 gtagcgatcc agccgcccac ggggccggat cccaggaagg acaatccgat tcccacaagg
      421 aggcccacgg ctccaccggc cagccagcgt cgagatcgct gttctcgtag cgcggtcacg
      481 gccaatggtt ctttcgagca atgacgatga aatcgcgaac gtctcggtct agtttatttt
      541 ccgctgtaat tgcggatgct tcccgatcat cccccacgtt atcgtcgtat acgtaatcgg
      601 aggcgtacag gatgacagca cgcaagccgt cgtcagggcc ttctcctgct cgcacgatga
      661 gttctgagcc ggtggaggat cgtttggcca gcggattgtc cccgagttcg tggacatcgg
      721 aagcggtgag atacaccacg ccgagttttc gtccctcggc tccgacgtcg agtcttacct
      781 tggtgttgcc agaaagcgat acccaccgag gtgctgtggg ttggatgcct tcggcggaaa
      841 aagcccgtgc gatgagtttg agagcgcgac cttcatcgac ggaccgcagc gggtttggtg
      901 tacgggcttc gggggggccg cagccgacgc tactgagata aaccaagatc gaagcgccca
      961 gggagagcat ggcgcgctga gccacgagtt gtgatttcat gcacgccaca gactagcgga
     1021 cggagccaag atgcgccaga tccggttgtt cgacccccca tccattcgac catgcacgga
     1081 acactttgcc atcggccttg ggtttcgtag tgttgaacca gaatggttcg gactcgcttg
     1141 tcatatcaag gtctgctgta ttgcatcctc atctcgaccc tgggcggatg cgcctctcag
     1201 cgcatatccg atcctcggtc cgccgcggaa gcctatgcac aagcggcctc gcggggagac
     1261 ggcgacatga tttacgacat gctcgacgag caggcacagc gttcgatgac gcgagcggag
     1321 gttcgaaagc tggtggcgaa tcagcgcgaa gagctagcgg agcaagccaa agcgatccgc
     1381 tctccccagg ctcgtgttcg atccaccgcg acgattcgct ttgcggatgg tgaagatgcc
     1441 attctggatc tatccgaaaa tggacagttt ctcattgtct cggccgatgc gttgcctgcc
     1501 ggcgcgcgaa cgccagtcca agctctggat cagcttcggc acgtgctagc tcgacgcagc
     1561 tacccgggtt tgttgcgggt gttgagcact tcgactcgca gtgcgatgga aaatgatctt
     1621 cgttctttgg tggagggatt ggagaatcct gaagaactcg acgtgcagca atctggcgaa
     1681 accgcgacgg tgcgcattcc cggtggccac tggatcgtgt tacgtcgcga gggagcaacg
     1741 tggcgtatcg acaacatcga ttgacatggt tttgggtgct ggcttgcctt ttgtttgtca
     1801 gctcgatccc cggcaaggtt cgcgcgatgg gtgacgaggg agcgtttcat ccccgggtac
     1861 tgatcgtggg agaggctcgc tttgaaggcg ctcgaaccac tgcacccgcg aggtggtcct
     1921 gggaattgac acgaagaacg agcgctcccg cgcgtcttgc tccgacgacg gttcgcgcgg
     1981 attctcccac cttgctcgag gagccttttg tgtggtggtt gggttccacg gacgtgggcg
     2041 ctctatcatc ccgtgaaatc acgggccttc gcgctttttt tgctcttgga ggcgtattgt
     2101 tcgtcgacga ttccgatccc accactggag tattcgaacg aagcgccaaa cgcgaac
//
LOCUS       DAOSOR010000774         2151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_51211_length_2151_cov_4.080630, whole genome shotgun sequence.
ACCESSION   DAOSOR010000774 DAOSOR010000000
VERSION     DAOSOR010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51211_length_2151_cov_4.080630"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1530)
                     /locus_tag="PK710_24755"
     CDS             complement(<1..1530)
                     /locus_tag="PK710_24755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13016.1"
                     /translation="MKTKRLLISTTAGFAALTATAVARADTVVDDFENFSGWTGLVAE
                     TKEVHDGKGAGRWQDHVNHSTAKKVFATPLDVSGEKHFQMWVYSAVANNAKITLVFDS
                     ENAADHEGWDYYSKSIYIDWTGWRFIRIPLDSFGVSRNPLGWNHINYISLHAGGWGTD
                     PKDDTLLILDSLSFGTGVISSVKMRPSWKGNDFEYRFEVGLEERLGKSRNLSFSLDYP
                     SSYPFSSSLDSSSLTLSPHGSGTLLAQVTVPSSAISGTTLLDIHSAFMVVSEQGLPID
                     GVQLDGAVPLVERDPPRTLLDAQDFARIEAWSASEAWASNARASILNQAEGWPEAYEK
                     KYFLSTWALPPEGGQWSLWYVCPVHGVNLKYEDKHHRCPVDGQEFTGWPYDQVIYGRM
                     HGDLAKYATDLGLAWRMTGQARYCQSALEILEAYADVYDSYPIHDIHQKQAGSGARVL
                     AQTLDEAGWLLPMAWAYDMVANCPAMTSGLKDHIEQDLLRHAVAVIQRHPAGESNWQS
                     WH"
BASE COUNT          462 a          639 c          592 g          458 t
ORIGIN      
        1 atgccacgac tgccaattgc tttcccccgc cggatgacgc tgaatgaccg ccaccgcatg
       61 acgcaacaaa tcctgttcga tatgatcctt caacccgctc gtcatcgccg ggcaattcgc
      121 caccatgtca tacgcccacg ccatcggaag caaccagccc gcttcgtcta gcgtttgcgc
      181 caagacgcga gccccggagc cagcctgctt ctgatggatg tcatgaatgg gatacgaatc
      241 atacacatcc gcatacgctt caagaatctc cagggcgctt tgacaatacc gggcttgccc
      301 cgtcatgcgc caggcaagtc ccaaatccgt cgcgtatttg gccaaatctc cgtgcatccg
      361 cccgtaaatg acctgatcgt aaggccaacc cgtgaattcc tgaccgtcga ccgggcatcg
      421 atgatgcttg tcttcatatt tcagattcac tccgtggacg ggacatacgt accatagcga
      481 ccattgccct ccttccggag gaagggccca ggtggataga aaatactttt tttcgtaggc
      541 ttccggccac ccctccgcct gattgaggat gcttgcccgc gcgttcgaag cccaggcctc
      601 cgacgcgctc cacgcctcaa tcctggcaaa gtcctgtgcg tcgagaagtg tgcgaggcgg
      661 gtcccgctcg accaggggaa cagccccgtc aagctgcaca ccgtcgatcg gaagcccttg
      721 ctcggacacg accatgaacg ccgaatgaat gtccaggagc gtggtgccgg agatcgctga
      781 cgatggcacg gtcacttgag ccaatagggt tccggacccg tgagggctca gggtcagcga
      841 ggatgaatcg agcgaggaag aaaaggggta ggaggaagga taatcaagtg aaaacgatag
      901 gttacgtgac tttccaagcc gttcttcgag ccccacttcg aaacggtatt cgaaatcgtt
      961 tcccttccag gatggccgca ttttcaccga ggaaatgact cccgtgccga acgacaggct
     1021 gtcgagaatc aacaaggtgt catccttggg gtccgttccc cagcccccgg catgcaagga
     1081 aatataattg atgtggttcc acccgagggg attgcgagac acgccgaagc tatccagggg
     1141 gattcggatg aaacgccatc ccgtccagtc gatgtaaatg gatttggaat aatagtccca
     1201 cccctcgtgg tccgcggcgt tttccgagtc aaaaacaagg gtgatcttgg cgttgttcgc
     1261 tacggccgag tacacccaca tttgaaagtg tttttcaccg gacacatcaa ggggtgtggc
     1321 gaataccttt ttcgcggtgg aatggttgac atggtcctgc caacgccccg cgcctttgcc
     1381 atcgtgaacc tctttcgtct ctgcgaccag tccagtccaa ccagagaagt tctcgaagtc
     1441 gtccaccacg gtatcagctc gcgccacggc cgtggccgtc aacgccgcaa aacctgccgt
     1501 ggtcgagatt aaaagacgct tggttttcat ggttatcccc atcacgtcgc tacgtagatg
     1561 aagtaagata gcaggtttac agtcggagtg gaggtgtaac cgacgcttcg agcatgactg
     1621 tggcttcgtc gagcgcgtag cgcgaaatcc agaagttcgc tcgcggcggg ggcaagggtg
     1681 tgtgtttgct tacgacaggg ctggcttgga gcggttttca ccaccgggcc ttcgggaagt
     1741 ccgaatgccg attgaacctg gggaccaggt cctcgacagg agcggattgt tcctccgacg
     1801 ggtcaccggg aaagggttag cgaaggtcgc taggctggct ttcggtcatc cgaatggcct
     1861 cgttcgagcc tgctagccgt ttgactgctc ctgagacatg gacggcagcc gttgcgccat
     1921 ggataggacg ccgttgccat gaccttgcca tctccttgtc acgccatggc gataggcaga
     1981 ataacgttga ccccaaaacc caacgctcat ccagcgtttc gcttacgcga ggtagtcacg
     2041 aaccaagggg tgcttttctc cgtaattcgt gagtgtctca ccgctgcctt ctcgaaactc
     2101 caccgcccct gccccggtcg ctccgccccc atcgatgcgt ttcgaacgac c
//
LOCUS       DAOSOR010000775         2133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_51804_length_2133_cov_4.213186, whole genome shotgun sequence.
ACCESSION   DAOSOR010000775 DAOSOR010000000
VERSION     DAOSOR010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51804_length_2133_cov_4.213186"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PK710_24760"
     CDS             complement(<1..445)
                     /locus_tag="PK710_24760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13017.1"
                     /translation="MTWLIALMVLAGVFVLLRALARKKQNAIHTIEPGLTQDEPTSDS
                     ASGGSKSQLEPKTTQSLRKQTIDERLLPKAPSRGPHHPRSRLFDAASARRRFSHSMRC
                     KNRDLSVLAEDPEQLARHGLPLWRNEAQLAEALGLSVGQLRHLSMH"
     gene            complement(611..>2133)
                     /locus_tag="PK710_24765"
     CDS             complement(611..>2133)
                     /locus_tag="PK710_24765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13018.1"
                     /translation="LLGLAANDHRPVRLVARVRNPLLFRFCLQTLGSLVWSDDTWISD
                     DEWTAFTLDPVVTVHPDRIMFEAFSRDQSAYGLLIIDRDQFETSGQVSCGTTNVDFTA
                     WLYGALAEMRSSRTTTFRVEAGGIEVSTRQAGGRFEQKVELPESWVRGFLNISAAMAF
                     PGTRLRAEPVDMLAVIRYLQQHKAKTAPRALRYEMIPGREAEIVVEPFETRFPLVGAN
                     HGYPKPRIIRTWGRRRLRLIEPLLPYAEHVEIYLKGRAMPSFYAVGLPGYTFVLGLSG
                     WTANSLTTGTGFAAGSSVSDSAVDRALMLLQQQYHIDVETLCAAATMDKPTAMAALET
                     LIRRGRAVFDVQARRVRYRELLAEPIDEAKIYPPDPRLVQVERWHREGAVHIRDTILR
                     KTTKTRQFKLDGQWIQRQVDLRDRVLTGRVGEQEEVEVVLDDEGRIIFGRCGCDHFVS
                     YALSKGPCAHLMALERQSRRHLHDPSALKSTANPISSLPNEGTEVLLDDNNEYDDE"
BASE COUNT          471 a          628 c          587 g          447 t
ORIGIN      
        1 gatgcatcga taggtgacga agttgaccta cgctgagccc aagggcctcg gccagttgcg
       61 cttcgttcct ccaaagcggc aatccatgac gcgcaagttg ctccgggtct tcggcaagca
      121 ccgagagatc tcggtttttg caacgcatcg aatgagaaaa ccgtcgacgt gccgatgcag
      181 cgtcaaagag tctgcttcgt gggtggtggg ggccccgaga aggggctttg ggcaacaagc
      241 gctcgtcgat ggtttgcttg cgaagcgatt gtgtcgtctt gggttctagc tgagatttgg
      301 acccgccgct ggcgctatcc gacgtgggtt catcctgggt gagtccgggt tcgatggtgt
      361 gaattgcgtt ttgtttttta cgtgcgagag cgcgaagcag cacaaacact ccggcaagga
      421 ccatcaacgc gatcagccag gtcatggaca aatgctcccg gaatcgcctg aaccacttcg
      481 aggctctggc accatggctc cccacgttac gcacgtgggg accgtggcgg tggaaaggag
      541 gcaacattcc actgcggcga aacaccgcag catgagaggc tggttctgca aagcgcttcc
      601 gagagcgggt tcattcatca tcgtactcgt tattgtcgtc aagaagaact tctgtccctt
      661 cgttcggaag cgaagatatc gggttagcgg tcgacttaag tgcggacgga tcgtgcaaat
      721 gtcgccggct ctggcgctcg agcgccatga gatgcgcgca aggacctttc gacaacgcat
      781 acgaaacgaa gtgatcacaa ccacatcgcc caaagatgat tctgccttca tcatcgagca
      841 cgacttccac ttcttcttgc tccccaaccc gtcctgtaag gacccgatcg cgcaggtcga
      901 cctgtcgttg tatccattgc ccatcgagct tgaactgacg agttttcgtc gtttttcgaa
      961 gtatcgtatc gcgaatatgc accgcacctt cgcgatgcca tcgctcaacc tgtacgagcc
     1021 gtggatccgg aggatatatc ttcgcttcgt cgataggttc cgccaagagt tcgcgatacc
     1081 gaacgcgacg cgcctgcacg tcgaataccg ctcgccctcg tcgaataagc gtttcgagcg
     1141 cagccatcgc cgtcggtttg tccatggtag cggccgcgca aagtgtttcc acgtcgatat
     1201 ggtattgttg ctgaagcagc atcagcgccc tgtcgacagc agaatccgac acagacgaac
     1261 cagcggcaaa accagttcct gtcgtcaaag aattcgctgt ccatccactg agccccagca
     1321 cgaacgtata gccgggcaaa cccacagcat aaaaactcgg catcgcgcgt cccttgagat
     1381 aaatctccac gtgctcggca taaggaagca agggttcgat aaggcgcaaa cgacgtcgcc
     1441 cccacgtgcg aatgatccgt ggttttggat aaccgtgatt ggcccccacc agagggaatc
     1501 gtgtttcgaa gggctccacg acgatctccg cctctcgacc gggaatcatt tcgtagcgaa
     1561 gtgcccgtgg tgcggtcttg gccttgtgtt gctgcaagta acgaatgacc gcgagcatgt
     1621 cgaccggttc ggcgcgcaac cgtgttcccg gaaatgccat ggccgcggat atattcaaaa
     1681 acccgcggac ccagctttcc ggcagttcca ccttctgttc gaagcgtcct cccgcttgcc
     1741 gcgtgctcac ctcgattcca ccagcctcca cccgaaacgt ggtcgttcgg ctcgatcgca
     1801 tctcggccaa ggcgccatac aaccaggccg taaagtccac attcgtcgtt ccacaagaca
     1861 cttgccccga cgtttcgaat tggtcccggt cgatgatgag caggccgtag gcgctctggt
     1921 cgcgagaaaa tgcctcgaac atgattctgt ccggatgcac cgtcaccact ggatcgagag
     1981 tgaacgccgt ccactcgtca tccgatatcc acgtatcatc ggaccagacc agcgaaccaa
     2041 gggtctgaag acaaaagcgg aacaagagag gattgcgcac gcgcgcaacc aagcgcacag
     2101 gacgatgatc attggccgcg agacctagca gtt
//
LOCUS       DAOSOR010000776         2123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_52139_length_2123_cov_3.742263, whole genome shotgun sequence.
ACCESSION   DAOSOR010000776 DAOSOR010000000
VERSION     DAOSOR010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52139_length_2123_cov_3.742263"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            306..1139
                     /locus_tag="PK710_24770"
     CDS             306..1139
                     /locus_tag="PK710_24770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13019.1"
                     /translation="MGKALRWATLAVLVMGCSGTVESANGGSAGASGVGGSAAMGGAG
                     GSHTGGTGASAGQAGNTGGNAADAGGGAGGSGAVAGGGAGGSGADAGASGQGATGGSA
                     GYPPAPQCKDDSQCILYSDCCTCVAMRPGDPVEDCPITCVVDKCTELGISQAKGPRCL
                     AGHCAVGFDCDAPKVLCNALPPQCEAGEVPSVNNLCWGPCVPASQCAFVPNCSNCDPK
                     MYTCVTDVGWVFEHHCVPTPQACKNDLSCECMQNAVCLPPFDHCSASPTDGVFCECTN
                     C"
     gene            1273..>1545
                     /locus_tag="PK710_24775"
     CDS             1273..>1545
                     /locus_tag="PK710_24775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13020.1"
                     /translation="MNKHVALTILAAAIAFTSSACTQKYLEFSVWEGPSQVSQAKKKF
                     VGGIGGYHDEAWALLEKNDVDGTIALIDGSSSKNYFDWYNLAVLYTV"
     assembly_gap    1546..1555
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1776..>2123
                     /locus_tag="PK710_24780"
     CDS             1776..>2123
                     /locus_tag="PK710_24780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13021.1"
                     /translation="MGSLGPALGSKVSEDRFPLHEEPVGSGALLAEPTMKAVVVRRRE
                     RGVRGVVRAREMPKEPSTTMNDGAEVEVGAAMVVASRVTQKIVWAAKEVGEAEQHVCP
                     SNAPRLAEALSAKR"
BASE COUNT          422 a          521 c          688 g          482 t
ORIGIN      
        1 ttcgattgag ggcacggtgg tttggcttgc cgcaagcgtg ttggcttgcc ctcttgatat
       61 acgattttct ttgatcgcga tggcgctgtc catggtgatg agtaattgca ccgtgcggct
      121 ggcttggatg cgttttccgt gacatcgtta tccctgcgtg aggtgggagg ctttttcagg
      181 gatgactgtc atggctttgg gtatgcgaat actctgggac gggaccgaat caaaacgtcg
      241 acgctcgagc catgacatga tggtgtgtcc gatggattcc atggttccgt tcaaggagaa
      301 tcaagatggg taaggcgctg cgttgggcca cgttggccgt gttggtgatg ggttgtagcg
      361 gaacggtaga gtcagccaac ggaggatcgg ctggcgcgag tggtgttgga ggctcggcgg
      421 ccatgggagg ggctggcgga tcgcacaccg gtgggacggg ggcaagtgca gggcaagcgg
      481 ggaatacagg aggaaacgcc gcggatgcag gtgggggtgc tggcggaagc ggggcggtag
      541 cgggtggggg tgctggcgga agcggggcgg atgcgggtgc ctctgggcaa ggcgcaacgg
      601 gaggttctgc aggataccct ccagctcccc aatgcaagga tgactcccaa tgcatccttt
      661 actcggactg ttgcacctgt gtcgcgatgc gaccgggaga ccctgtcgaa gattgtccca
      721 tcacgtgcgt cgtcgataag tgtaccgaac ttggcatcag ccaggcgaaa ggtccccgtt
      781 gtctcgcagg ccactgcgcc gtgggctttg attgcgatgc ccccaaggtg ttgtgcaacg
      841 cccttcctcc ccaatgtgag gctggcgagg tgccaagcgt aaacaatctt tgttggggcc
      901 cttgcgttcc cgcatctcaa tgcgccttcg ttccgaattg ctccaactgc gatccgaaga
      961 tgtacacgtg cgtgacggat gtcggatggg ttttcgaaca tcattgcgtg cccaccccgc
     1021 aggcctgcaa aaacgatctg tcctgtgagt gcatgcaaaa tgccgtctgc ctaccgccct
     1081 tcgaccactg ctctgcgtcg cccacggacg gcgttttttg cgaatgcacg aattgttgaa
     1141 ggcgtaactt attgaatttc aacgatatta ttaagattcg aagcgctctc cgcattgcta
     1201 tggtacttag cgcgtgtttt ccgtaaactc ggggcccttc gaaccttgtc atcccacgga
     1261 ggagaccgtc tcatgaacaa gcatgtcgct cttacgattc tcgctgcagc cattgcgttc
     1321 acatcatccg catgcacgca aaaatatttg gagttcagtg tttgggaagg gccttctcag
     1381 gtcagccaag ccaagaaaaa gtttgtgggt ggtatcggcg gataccacga cgaggcgtgg
     1441 gctcttctcg agaagaatga cgtagatgga accatcgctc ttatcgacgg ctcttcgagc
     1501 aagaactact tcgattggta caaccttgcc gtgttgtata ccgtcnnnnn nnnnngaagg
     1561 ctgaggaatg cattcagaag gcgatcgaag atcacgagaa gtccttcaac aagccgtatg
     1621 atatgttgca ggaagaactc gcctacatcc aagatcatcg cgctcgctac gtgagccaat
     1681 aatcatctcg aaccacgagt ttttcgactc gtgcggtccc atcccccatt tgcatcctat
     1741 cgtcctgccg gcgtgccctt tgcgttgatc gcatcatggg ttcgttgggt ccggctcttg
     1801 ggagcaaggt ctcggaagac cgctttcctc ttcatgaaga accggttggt tcgggcgcgc
     1861 tgcttgcgga gccgacaatg aaggcggtgg tggtacgacg gagggaacgg ggcgtgaggg
     1921 gcgtggtgcg ggcccgtgaa atgccaaagg agccatcgac gacgatgaac gatggggcgg
     1981 aggtggaggt gggtgctgcg atggtggttg cctcgcgggt gacgcagaag atagtttggg
     2041 ccgcgaagga ggtgggggag gcggagcaac acgtttgtcc ttcgaatgcg ccgagattgg
     2101 cagaggcatt gagcgcgaaa agg
//
LOCUS       DAOSOR010000777         2115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_52404_length_2115_cov_3.611650, whole genome shotgun sequence.
ACCESSION   DAOSOR010000777 DAOSOR010000000
VERSION     DAOSOR010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52404_length_2115_cov_3.611650"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PK710_24785"
     CDS             complement(<1..1171)
                     /locus_tag="PK710_24785"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOT13022.1"
                     /translation="MKHVMRWVAPLLLALPLGIACSSTDPENDPAEPTDDESRLRNEI
                     FEENASGKADNADCSGVVLPDQSGFNKRVAITFDDGPHLDNTPRVLDVLAAHHATATF
                     FINGKGVRTAQHRDLLKRMHEAGHIIGNHSQNHANLKTVSAATLKAEIEGTHQILLDV
                     GVKPAFFRFPYGSSSCATAETVRSYGYAVTGWHIDSADWCFAASRGGVGYCDPATFQH
                     VPNSYRSDIAGFVLSQARAKGGGVLLFHDVHAYTVGVLDSILTKLENDGFRFVGLDDV
                     DTFPRLNGKKPASSPFIGSACEDHDDCNFTAVNEAGFCQTYEGGGFCSISCNGTCPDK
                     AGMAPTFCVSLDGNSGQCVAKSSAANGYCADLPGTTAKPMSRFIGTSSAPASTADV"
     assembly_gap    1395..1404
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1413..>2115
                     /locus_tag="PK710_24790"
     CDS             1413..>2115
                     /locus_tag="PK710_24790"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOT13023.1"
                     /translation="MQANSGEPHPKVVVADDDRVTREAICSILRRAGHEVFPATDGQE
                     AVEIVGQGGVDLVLLDILMPRLGGIETCRIIKGMTVDTFVPVMLVTVRTDTASRVEGL
                     KIGADDYISKPFEERELIARIEAMLRIKRLNDHFVAVRQKLERLSVYDELTGLYNYRY
                     LHTRLQEEFKRAERYHEPLACVVIDIDKLHDHNEKGGQRHGDAILRGVADRIRRCVRE
                     VDVVARYGGEEFLVIL"
BASE COUNT          506 a          597 c          588 g          414 t
ORIGIN      
        1 aaacgtccgc ggtggacgct ggggcagaac tggtaccgat gaaccgactc attggcttgg
       61 ctgtcgtgcc gggaagatca gcgcagtagc cattggccgc gctggatttc gcaacgcatt
      121 gaccggagtt gccgtccaac gacacacaaa acgtcggggc catgccagcc ttatcgggac
      181 aggtgccatt gcatgagatc gagcagaagc ctccgccttc atacgtctga cagaaccctg
      241 cttcgttgac agccgtgaag ttgcagtcat cgtggtcttc acaagccgag ccgatgaagg
      301 gcgaggacgc tggttttttc ccgttgagcc gtgggaaagt gtcgacatca tcgaggccga
      361 cgaacctgaa gccatcgttt tcgagcttcg tgagaatgct atcgagcacg ccaaccgtgt
      421 aggcatgaac gtcgtggaaa agcagcacgc cgccgccttt ggcacgagct tgactcaata
      481 cgaatccagc gatgtcactc cggtacgaat tcggcacgtg ttggaaggtg gccggatcgc
      541 aatagcccac acctccacgg gatgccgcaa agcaccaatc cgctgaatcg atatgccagc
      601 ctgtcaccgc ataaccataa gaacgaacgg tctcagcggt ggcacaggaa gaagacccat
      661 aagggaaacg gaagaacgcg ggtttcaccc ccacatcgag caagatttga tgtgttccct
      721 caatctccgc cttgagcgtg gccgcggata ccgtcttgag attcgcgtgg ttttgcgagt
      781 gattgccaat gatatgcccc gcttcatgca tacgtttcaa aaggtcacga tgttgggcag
      841 tccttacgcc tttgccattg atgaaaaacg tagcggtggc gtggtgcgcg gccagcacgt
      901 cgagaacccg cggcgtattg tccaaatgag gaccatcgtc gaacgtgatg gccacacgct
      961 tattgaatcc gctttgatcg ggaaggacaa cgccggaaca atcggcgtta tcggcctttc
     1021 ccgacgcatt ttcctcgaat atctcattgc ggagtcgaga ctcgtcatcg gtcggttccg
     1081 cgggatcgtt ttccggatcg gtggaagaac aagcaattcc caagggaaga gcaagaagca
     1141 agggggcaac ccaacgcatg acgtgcttca tggtctcagt cctcatcgcg cgggacacca
     1201 acgagcggtg ccccggttat aaacgttaat cgttgcacta tatccaagtg tctgcatcat
     1261 gtcttcaaaa atgcagtgac aaaagaagaa aggggtgcgg gttgcagcca ggtcaaggtt
     1321 cgcgcaacct agccccgtgc ctgccgaaaa aatcatcaat caccgcccaa gtgacccccc
     1381 cccccccccc ccccnnnnnn nnnnattggg cgatgcaggc aaattcaggc gaaccgcacc
     1441 ccaaggtggt ggtggccgac gatgaccgcg tgactcgaga ggccatctgt tccatcttgc
     1501 gccgggctgg acatgaggtt tttcccgcca cggacggaca agaagccgta gaaatcgtgg
     1561 gccagggggg cgtggacctc gtgctgctcg atatcctcat gccccgactc ggcggaatcg
     1621 aaacatgccg gatcatcaag ggaatgaccg tcgacacctt cgttcccgtc atgctcgtca
     1681 ccgtgcgaac cgataccgcc agccgtgtcg aaggcttgaa aattggagcc gatgactaca
     1741 tcagcaaacc cttcgaagaa cgggaactca tcgctcgcat cgaagccatg ctgcgcatca
     1801 aacgcctcaa tgatcacttc gtcgccgtac gacaaaaact cgagcgactc agtgtctatg
     1861 acgaactcac gggactctac aactaccgat acttacacac acgactgcag gaggaattca
     1921 aacgcgccga gcggtaccat gaaccgttgg cgtgtgtggt catcgatatc gataagcttc
     1981 acgatcataa tgaaaaagga gggcagagac acggcgacgc catccttcgc ggcgtggcgg
     2041 atagaatccg acgatgtgtg cgcgaagtcg atgttgtcgc acgctacggc ggagaagagt
     2101 tcttggtcat tctgc
//
LOCUS       DAOSOR010000778         2109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_52588_length_2109_cov_3.590068, whole genome shotgun sequence.
ACCESSION   DAOSOR010000778 DAOSOR010000000
VERSION     DAOSOR010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52588_length_2109_cov_3.590068"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /gene="mreC"
                     /locus_tag="PK710_24795"
     CDS             complement(<1..670)
                     /gene="mreC"
                     /locus_tag="PK710_24795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOT13024.1"
                     /translation="MNSLRRYRDAIIVVILLAVPFFFLRANVRDPRELNPLDRAIITI
                     SAPFQYVASMLARGISTIVGDYIYLVDVKADNQKLAYENARLHARVRHLEHLEVDNAR
                     LKKLLELKNTLPADLVSAHVVSKDTTEYFRVARVSLDRSARNIRPNMPVLALDGVVGT
                     VHRVAGDTIDVLLTVDSGSGIDVVVERTGARGIVRGTGDRSRYACRVEYVQRTDEVAV
                     GDLLL"
     gene            complement(707..1741)
                     /locus_tag="PK710_24800"
     CDS             complement(707..1741)
                     /locus_tag="PK710_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOT13025.1"
                     /translation="MIFDWLYGLFSNDLAIDLGTATTLIYVRGKGIVSCEPSVVAVQQ
                     DARGAKKVLAVGREAKEMLGRTPGNIQAVRPLRDGVIADFEITEAMLRWFIARANTRR
                     TLVKPRIIICVPFGITEVEKRAVKESAEGAGAREVYLIEEPMAAAIGAGLPITEPSGN
                     MVVDIGGGTTEVAVISLAGIVYSQSVRVGGDKMDESIVAYMKRKYNLAIGEQTAERIK
                     VTIGNAYPLEQQATMEVKGRDMVAGVPKTIVVHSDEIREALSEPINAIVDAVLIALER
                     TPPELAADIVDKGIVLTGGGALLKNLDVLLREESGLPVMVCDDPVSAVVLGSGKALDH
                     MELLKEVTIG"
BASE COUNT          485 a          626 c          544 g          454 t
ORIGIN      
        1 tcaacaacag atcgcccacg gcaacttcat ccgtgcgttg aacgtattcc acgcgacaag
       61 catatcgcga acgatccccc gtgccacgaa cgataccacg cgcccccgtg cgttcgacca
      121 ccacatcgat gccgctgccc gagtctaccg tgagcaaaac atcaatggtg tcacccgcca
      181 cgcggtgaac ggtgcctacg acaccatcga gagccagcac cggcatattg ggccggatgt
      241 tgcgtgcgct tcgatccaaa gacacacggg caacacgaaa atactcagtg gtatctttcg
      301 atacgacatg agcagaaacc agatccgccg gcagggtgtt ctttagttca agcagcttct
      361 tgagccgcgc gttatccact tcgagatgct cgagatggcg aacccgcgcg tggagacgcg
      421 cgttctcata cgccagcttc tgattgtccg ccttcacatc gaccagataa atataatccc
      481 ccacaatggt ggaaataccg cgcgccagca tggatgcgac atactgaaac ggcgccgaaa
      541 tggtgatgat ggcccgatcg agcggattga gttcgcgcgg atcccggacg ttggcccgca
      601 ggaaaaaaaa aggcaccgcc aagagtatga cgacgatgat tgcgtctcga tagcgcctta
      661 gtgaattcac gatcaggttg ctccggtcgc gatcaggtcg cgtccgctac ccgatcgtta
      721 cctccttgag gagttccatg tggtcaaggg cctttccact tcctagcaca actgcggaga
      781 cggggtcgtc acacaccatg acgggaaggc cggattcttc acgaagaagg acatcaaggt
      841 tttttaatag ggccccccct cccgtaagaa cgattccttt gtcgacgata tcagcggcta
      901 actcgggagg agtacgttcc agcgcgatca gcacggcgtc gacaatggca ttgatcggtt
      961 cactcaaagc ttcccgaatc tcatcggagt gtaccacgat ggtttttggt accccagcca
     1021 ccatgtcgcg tcctttgact tccatcgtcg cctgttgctc gagcggataa gcgttgccaa
     1081 tggtgacttt gattcgctcg gcagtttgct ctccgatggc caagttgtat ttgcgcttca
     1141 tatacgccac gatggattca tccatcttgt ctccgccgac ccgaaccgat tgcgaataaa
     1201 cgattccagc cagggaaata acggctactt cggtggttcc gccgccgatg tcgacaacca
     1261 tattgccgct gggttcggtg atgggtaacc ccgccccaat ggcagcagcc ataggttctt
     1321 cgataagata tacctcacga gcaccagcac cttccgcact ttccttgacc gcccttttct
     1381 cgacctccgt gatcccaaac ggtacgcaaa tgatgatgcg cggcttcacc agcgttcgtc
     1441 gcgtgttggc ccgagcaatg aaccaccgca acatcgcttc cgtgatttca aaatccgcaa
     1501 tgaccccgtc gcgtaacggt cgaaccgctt gaatgttgcc cggtgtgcga cccagcatct
     1561 ctttggcctc tcgtccgacc gccaacactt ttttggctcc tcgcgcgtct tgttgcacgg
     1621 caacgacgga cggctcacaa gatacgattc ccttgcctct cacgtagatc agcgttgtcg
     1681 ccgtgcccag gtcaatggcg agatcattgg agaaaaggcc gtagagccaa tcgaagatca
     1741 tttcgtagcc tctgcggtcc ggcgatggct catggcagac aatgggtagc acaggctcgg
     1801 atggccgacg ttcgtctgag tatcctgcga agggttcaga atcataaacg ctggaagatg
     1861 agcttcatac gagcgaatca ttgtgtgcgt cgccaattca ggcgcgtctc gtaacacgcg
     1921 actccaaacg aagcaaattg agcatcacaa gggctgctta tgcgttcaga caaaggggga
     1981 aagatgactt cttcccttga ccttcaaaac caaatactac gtcagggccg acctgctggg
     2041 cgcggtttac atgcccgcaa cgacgcaagt caaggtgcag agatgcccgc tggtcagcaa
     2101 acgtcattc
//
LOCUS       DAOSOR010000779         2102 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_52844_length_2102_cov_3.385442, whole genome shotgun sequence.
ACCESSION   DAOSOR010000779 DAOSOR010000000
VERSION     DAOSOR010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52844_length_2102_cov_3.385442"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="PK710_24805"
     CDS             <1..907
                     /locus_tag="PK710_24805"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOT13026.1"
                     /translation="QTLIAAAVRPISWLSVGAGMRFAVETRSDNHIFVPSQADNSVQY
                     IDMRTETGVATAPVVSAATLLHDDRLHVSLTWRGELESSLRGANTVQINGFQGTPQYP
                     FRQPMNIIADYAPHQVLLSGAWKDDSFAATLDTTWSQWSGYRDDTDNLAGFHDTVSAA
                     AGVEGRVTKRSVVRGGLGFRPSPVPEQVGRTNYADNSMWIFGLGFGHEVEIDGATLDI
                     SAYGQVHAAVPRSHTKAFSSNAPACAEGVTALCDEIPDDIRDPVTGKEMPEVQGLQTG
                     NPGFPGWSSGGWIAVAGIQVSWRYR"
     gene            904..1812
                     /locus_tag="PK710_24810"
     CDS             904..1812
                     /locus_tag="PK710_24810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13027.1"
                     /translation="MKSSLFTLGSWALLACLWLSACDPPEPPAGEYPAVFRDAAATSD
                     AEAADSGPNSDLVADGTWLLWHETSTCVKALSVDLESVTQTLLLVSLSSDVGGVVHHT
                     SRDCLIEQTPIVGVATTIPLQVVETIPPQNYVAVLSGSHEGATYSTQRIIELWGLHLD
                     DPEFEELPKDPLDSRVYDQDGDGNPGATLVLGANQCSMYVVQRAIAQWNGKVESSIRI
                     AGVGNNATTQVVLGATGGFCASQFDTRNPPDSARFVLQRVDGRYGAPNLDTNGDGQID
                     CEEVRAYGSEPFGPRQPDNERCKSQP"
     gene            complement(1835..>2102)
                     /locus_tag="PK710_24815"
     CDS             complement(1835..>2102)
                     /locus_tag="PK710_24815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13028.1"
                     /translation="PGALFACAVKIASYPEIYHDSGLVTTVRDQGRPALRDLVGSWFP
                     SLTQFCFYQNGSLRAEKHKKWLENESALARLGRNAPDVPPEGTL"
BASE COUNT          394 a          550 c          677 g          481 t
ORIGIN      
        1 gcagacgctc attgccgccg cggttcgtcc catatcctgg ttatctgtcg gcgctggaat
       61 gcgttttgcc gtggaaacac ggtccgacaa ccatattttc gtgccgagtc aggcggataa
      121 ttcggtgcaa tacatcgata tgcgcaccga aaccggtgtt gccaccgctc cggtggtctc
      181 ggcggcgacc cttttgcatg acgaccgtct gcatgtatcg cttacgtggc gcggagaact
      241 cgagtcttct ctgcgcggtg ccaacacggt tcaaatcaat ggttttcaag ggactccgca
      301 ataccccttt cgccaaccca tgaatatcat tgccgattat gctccccacc aggtcctcct
      361 ttccggcgcg tggaaggatg attcctttgc ggcgacgctc gatacgactt ggagccagtg
      421 gtccggttac cgtgacgata cggataatct ggcgggattt cacgataccg tatcggcggc
      481 agcaggcgtg gaggggcgag tgacgaaacg ctcggtcgtt cgtggcggcc ttggattccg
      541 tccttccccg gtaccggagc aggtaggaag gacgaactac gccgacaatt ccatgtggat
      601 tttcggtctg gggttcggcc atgaggtcga aatcgacggg gcaaccttgg atatatcggc
      661 ttatgggcaa gtgcatgcgg ccgttccccg ctcccacacc aaagcttttt cgtcgaacgc
      721 tccggcctgt gcggaggggg tcacggcgct ttgcgatgag attcccgatg atatccggga
      781 tccggttacg ggcaaggaga tgccggaggt gcaaggccta caaaccggaa accctggttt
      841 tccaggatgg tcgagtggtg gctggattgc ggtggcgggt atccaggtgt cctggaggta
      901 tcgatgaaat cctcgttgtt tacccttggt tcatgggcgc tcctcgcatg cctgtggctt
      961 tctgcctgcg atccgccgga gccgccggcg ggggaatatc cggcggtttt tcgtgatgcc
     1021 gcggctactt ccgacgccga ggccgcggat tctggcccga attcggattt ggtggccgat
     1081 ggcacctggc tgctttggca tgagacttcc acgtgcgtga aggcgctgtc ggtcgatttg
     1141 gagtctgtca cccaaacgct gttgctcgta tcgctgagtt cggacgtggg cggggttgtc
     1201 catcatacgt cccgcgattg tctgatcgag caaacgccga tcgtcggtgt ggcgaccacc
     1261 attcccttgc aggtggtcga gacgatacct ccgcagaatt acgttgccgt gttgagcggc
     1321 agccatgagg gggcgacgta ttccacgcag cgtatcatcg agttatgggg cttgcatctc
     1381 gacgatccgg aattcgagga attgccaaaa gatcctttag attcaagagt ttatgatcag
     1441 gatggcgatg ggaatcctgg tgccacgctc gtgttggggg ccaatcaatg ctcgatgtat
     1501 gtggtgcagc gggcgatcgc gcagtggaat ggcaaggtcg aatcgagcat tcggatcgcg
     1561 ggcgtgggca acaacgccac gacgcaggtt gtgttggggg ctaccggtgg attttgcgca
     1621 tcgcaattcg acacccgcaa tcctcccgat tcggcgcgat tcgtgttgca gcgggtggat
     1681 ggtcggtatg gggcgccgaa tttggatacg aatggggatg ggcagataga ttgtgaggaa
     1741 gtgcgagctt atggcagtga gccctttggt ccccggcagc cggacaacga acgctgcaaa
     1801 tcccagccgt gatcgaggat ggggtttgcg ggattcagag ggttccttcg ggaggaacgt
     1861 cgggggcgtt tcgtcccaaa cgcgcgaggg cgctttcgtt ttcaagccat tttttgtgct
     1921 tttcggcgcg tagggacccg ttttgataaa agcaaaactg ggtcagagaa gggaaccacg
     1981 agcccacgag gtctcgtagc gcggggcgtc cctgatcccg caccgtcgtc accagtcccg
     2041 aatcatgata aatctcggga tatgaggcta ttttgacggc acaggcgaaa agggcgccag
     2101 gg
//
LOCUS       DAOSOR010000780         2086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_53365_length_2086_cov_3.203348, whole genome shotgun sequence.
ACCESSION   DAOSOR010000780 DAOSOR010000000
VERSION     DAOSOR010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53365_length_2086_cov_3.203348"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..841)
                     /locus_tag="PK710_24820"
     CDS             complement(164..841)
                     /locus_tag="PK710_24820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13029.1"
                     /translation="MRRFIIGASLTTLTCLGTPALAQQFAEKGAMALSADHLFGVTSY
                     KITLDPDIPYTDDIDVKGTVFSLLWGAQAGASPVVPAIYPRTSFDYFVIDGLSLGGSV
                     GFSTASVKVKSGGSSMEGDHTAVTFAPRVGYAFGLSDTIWFWLRGGPTYVSIEADPEY
                     SDVSEASALQFKAEGMFVFGISGGFGLEVGPALGLPLTGEFDAGRVDGDLSALHFGVY
                     AGMVGWF"
     gene            complement(924..1211)
                     /locus_tag="PK710_24825"
     CDS             complement(924..1211)
                     /locus_tag="PK710_24825"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOT13030.1"
                     /translation="MNRRPDGSPKGSCAERGSEQVYKALRAGLKERGLARVRARVVSC
                     SCLDACTTGPSILVEPQHIMYGRVTIEDIQDILDAIENGTVVERLSINPEF"
     gene            1370..>2086
                     /locus_tag="PK710_24830"
     CDS             1370..>2086
                     /locus_tag="PK710_24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13031.1"
                     /translation="MLRYNSFRWWCGWIVQLSWVVSLLMFVGCRNSSPSDKNAAAASI
                     DHPPGLPHPEMEANQVALEEPGGTRSGTPNGIPPALRGPRLANGEKGGGLGNHARSPG
                     APPLAAQESSPFMAGQPITAEPQLAIDPNGRFATTYRPGGGHLAAFESAVARGIVPGA
                     ERELVSDVGARYWTPMDSPRDRALALRTDFERAALPPSGGETHMRLSLRSSDRAAAAR
                     PHLSVHLVLDVSGSMRGEPMD"
BASE COUNT          491 a          660 c          530 g          405 t
ORIGIN      
        1 gctggtgatg aagggcactg gggttgttcg aagcaagtca tcacgggccg catcgacgtg
       61 tctcgacatc cccttcgcgc catgaatggc cacgcaacaa cctctgcgca tggtgggcca
      121 ggtccccccc atggcacaag cattctaccg tgtacgaccc gaatcagaac cagcccacca
      181 taccggcgta tacaccgaaa tgcagggcgc tgagatcccc atcgacacgc cctgcatcaa
      241 attctccggt gagcggcaga cctagcgcag gcccgacttc cagtccaaaa cctcccgata
      301 tcccaaacac gaacattcct tccgccttga actgcaacgc ggaagcttca gagacatcac
      361 tatactcggg gtccgcttct atcgacacat aggtgggacc accacgaagc cagaaccaga
      421 tcgtatcgct caacccaaac gcatatccca cgcgcggagc gaaggtaact gcggtgtgat
      481 ccccttccat gctactacca cccgacttca ccttaaccga agccgtcgaa aagcctacgg
      541 atccgcccag agaaagaccg tcgatgacga aatagtcaaa cgacgttcga gggtagatgg
      601 caggaacaac agggcttgcc cctgcttgag caccccacaa caaggagaaa accgttccct
      661 tgacatcgat atcatcggta tacggaatat ccgggtcaag cgtaatttta tagctcgtta
      721 ccccaaataa gtggtccgcg ctcagcgcca tcgctccttt ttccgcgaat tgttgtgcca
      781 atgccggggt acccaggcaa gtaagcgtgg ttagcgaagc tcctatgatg aatcggcgca
      841 tggcaattct cctgtccaca acaccaccta gctggacgaa aatactcctg ttggcatcga
      901 agttcaaaga caattcaaag tatttaaaac tctggattta ttgaaagacg ttcgacaaca
      961 gtaccatttt caatcgcatc aaggatatcc tggatatcct ctatggtgac tcgaccatac
     1021 atgatatgtt gcggctcgac caatatcgat ggccccgtcg tgcaggcatc caagcagcta
     1081 caagacacca cccgggcgcg tacacgggcc aatcctcgct ccttgagccc cgcccgcagc
     1141 gccttgtaca cctgttccga ccctctttcc gcacacgagc ccttcggact cccatccggg
     1201 cggcggttca cacaaaccat caagtattgg tctcgaagcg gcataaacgc ttatctgacg
     1261 gggccttgcc tcgacgtcaa gcacacaatt cacattcgag cccaatgaag gaaacatgac
     1321 cgtgtcaaat gtgtcaccat atcggctggc aaggctcagg agggttcgca tgcttcgtta
     1381 caattcgttt cgctggtggt gtgggtggat cgtgcagcta tcgtgggtag tctcgctgct
     1441 catgttcgtc ggttgccgaa atagttcacc cagcgacaag aatgccgccg ctgcaagcat
     1501 cgaccatccg ccaggactcc ctcacccaga aatggaagcc aaccaagttg ctctcgaaga
     1561 acccggcggc acaaggtcag gcacgcccaa tggcattcct cctgcgttac gaggaccgcg
     1621 actcgccaac ggcgaaaaag gtggtggcct gggaaatcat gcgcgatcac caggcgcgcc
     1681 accgctggct gcccaagaat catccccctt catggcagga cagccaatca cggccgaacc
     1741 acaattggcc atcgacccca acggccgatt tgccacgacg tatcgccccg gcggtgggca
     1801 tctggccgcc ttcgaatcag cggtggcaag gggaatcgtc ccgggcgccg aacgggaact
     1861 cgtcagcgat gtgggcgcgc gatattggac gccgatggac tctccccggg accgtgcctt
     1921 ggcccttcga acggacttcg aacgcgccgc acttccgcca agcggggggg agacacacat
     1981 gcgtctgtct cttcgttcca gtgatcgagc tgccgccgca cgccctcatc tttccgttca
     2041 tctcgtgctc gatgtcagcg gttcgatgcg gggagagccc atggac
//
LOCUS       DAOSOR010000781         2084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_53444_length_2084_cov_2.998029, whole genome shotgun sequence.
ACCESSION   DAOSOR010000781 DAOSOR010000000
VERSION     DAOSOR010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53444_length_2084_cov_2.998029"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PK710_24835"
     CDS             complement(<1..257)
                     /locus_tag="PK710_24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOT13032.1"
                     /translation="MMESAGRTALRAAIQANYESCLSCHACRTQCPVHVATGLLAPLG
                     LLRMAALGMGERLKQASELWYCLQCRRCTLACPSEVAPSTL"
     gene            complement(254..>2084)
                     /locus_tag="PK710_24840"
     CDS             complement(254..>2084)
                     /locus_tag="PK710_24840"
                     /inference="COORDINATES: protein motif:HMM:NF013314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT13033.1"
                     /translation="WRSKNPSLLSPYDQLEAAPGVLVCGSALEPTDIAGAVVGGTAAA
                     SRAVQALLRLGRTTASSQPPAFREVDETEKTLVLVCQCHLDGLAGLPPQVPGADVRLV
                     PSPCLPGGMENLRSLLRSTHHPRVVFAACRPHAERRTFERLAQREGVDEAYRQVVSDR
                     LSQTALTMAVAALEANQPVPDEAVPQSRLFPRVLVVGGGISGMQAAWSLAERGIDVDL
                     VESRTHLGGMWSRDGFGPEPVVEAMKALETEVRDAERIRVHLGTRLVATERVPEGWIS
                     TLQQDHQQVAVWHGATILAVGAKEKSIKSYCYGDCDRVLTQTRFERSLQNHELDPGSV
                     VVMIQCVETRQGGASDVCSRVCCLRALRHAIFLRRTDPRSRVVIVYRDMMAWGRWEQE
                     YTLARRLGVVFVSYHPDRPPQVSLHEDKPVVQVWDPVLGHAVRVTADWLVLSSSLEAS
                     VSNGELARVLGLELDGLGYFREVDPKWRPVDTAVDGVYLAGTCHSPQLAGEALLQAQA
                     AAQRAYERLTMPPNVLPSTVATVRHELCARCGLCIEACVFKARWLDPSEDRVVVDAMA
                     CRSCGACVSACHNGSARLASMGEGAWMAQVEASMSLISLGKNR"
BASE COUNT          463 a          761 c          511 g          349 t
ORIGIN      
        1 atcaacgtcg aaggcgccac ttcggaagga cacgccaacg tgcatcgacg acattgcaaa
       61 caataccata gctcagacgc ttgcttcagc cgctccccca tccccaaagc cgccatgcgc
      121 agcaacccca agggagccaa tagccccgtg gcaacatgca cagggcattg cgttcgacag
      181 gcatgacaac tcaaacatga ttcgtaattc gcctgaatcg ccgctcgtaa cgctgttcgc
      241 cccgcgcttt ccatcatcga tttttcccta atgaaatcaa agacatggac gcttccacct
      301 gcgccatcca cgccccctcc cccatcgacg ccaaccgcgc ggacccgttg tggcacgcgg
      361 atacgcaagc cccacacgac cggcaggcca tcgcatcgac cacgacacgg tcctccgagg
      421 gatccaacca cctggccttg aaaacacaag cctcgataca caaaccgcaa cgagcgcaaa
      481 gctcatggcg aaccgtggct accgtcgaag gcaatacgtt cggcggcatc gtcaaacgct
      541 catacgcccg ttgcgcggcc gcctgagctt gcagcaatgc ctcgcccgca agctgcggcg
      601 aatggcacgt tcccgccaaa tacactccat ccaccgccgt atccacaggt cgccacttcg
      661 ggtcgacctc acgaaaatac cccaacccat ccaattccaa tcccaatacc ctcgccagct
      721 caccattcga taccgacgct tccagcgacg aactcaatac caaccaatcc gcggtcacac
      781 gcacggcgtg ccccaacacc ggatcccaca cctgcacaac cggtttatcc tcatgaaggc
      841 tgacctgcgg cggccgatcc ggatgatacg atacaaagac aacccccagt cgacgcgcca
      901 acgtatattc ctgctcccat cgtccccagg ccatcatatc ccggtacaca atcaccaccc
      961 gggaccgcgg atccgtacgt cgaagaaaaa tggcatgacg aagcgcccgc aagcagcaca
     1021 cccgactgca aacgtcggat gcgccccctt gccgcgtctc cacgcattga atcatcacga
     1081 ccacactgcc gggatccagt tcgtggttct gcaaggaacg ctcgaagcgc gtttgcgtca
     1141 gcacacgatc gcaatcaccg taacaatatg attttattga tttttctttg gctcccaccg
     1201 caagaatcgt ggccccatgc cacaccgcga cctgttgatg atcctgctgc aacgtgctta
     1261 tccacccttc gggcacgcgt tcggtcgcca ccagccgggt ccccagatgc acgcggatgc
     1321 gctcggcgtc acgaacctct gtttcgagcg ctttcatggc ttccacgacg ggctcgggcc
     1381 cgaagccgtc gcggctccac atccctccca aatgcgttcg tgactccacg aggtccacat
     1441 cgatcccgcg ttccgccagg ctccaggccg cctgcatccc cgagattcct ccgcccacga
     1501 cgagcacccg ggggaacaat cgagactgtg gtaccgcttc gtccggcacg ggctggttcg
     1561 cctccaaagc agccaccgcc atcgtcaaag ccgtctgcga tagccggtcc gagaccacct
     1621 gccgatacgc ctcatccaca ccttctcgtt gcgccaaccg ctcgaacgtg cgtcgttccg
     1681 cgtgaggacg gcaggcggcg aacaccactc gtggatgatg cgtcgagcgc aacaggcttc
     1741 gaaggttttc catgccgcct ggcaaacatg gcgatggcac gagtcgcaca tcggctcccg
     1801 gcacctgggg cggcaaacca gcaagcccgt ccagatggca ttgacacacc aacaccagcg
     1861 ttttttccgt ctcatccact tcgcgaaacg cgggcggctg ggatgacgcc gtggttctgc
     1921 ccagtcgaag aagcgcttgc acggcccgcg acgccgccgc ggtacccccc accaccgctc
     1981 cggcaatatc cgtgggttcg agggccgagc cgcagactag cacaccaggg gcggcctcga
     2041 gctgatcgta gggagaaaga agcgaaggat tcttcgaacg ccac
//
LOCUS       DAOSOR010000782         2050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_54664_length_2050_cov_4.044612, whole genome shotgun sequence.
ACCESSION   DAOSOR010000782 DAOSOR010000000
VERSION     DAOSOR010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54664_length_2050_cov_4.044612"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PK710_24845"
     CDS             complement(<1..231)
                     /locus_tag="PK710_24845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13034.1"
                     /translation="MWKRNRWLGRTGVAVMVAALALVSSRSSMAQEASAEVSTSGFSK
                     ATATGNTDHDLFAGSFAVGYFGVRSVPIAINPQ"
     gene            431..1849
                     /locus_tag="PK710_24850"
     CDS             431..1849
                     /locus_tag="PK710_24850"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HOT13035.1"
                     /translation="MLRRIVLFATPALFTLLACQGPTQPQSDAIRYLGQGIMGGADDP
                     DHPAIVGIISIQGMGAGLCSGTLIAPNLVLTARHCVAETQEQVSCTSSTFGKTFGASS
                     FFITTEWQGPEIAYQTYELKGNWFQAAEIIVSDDNHVCGNDIALIRLATNVPNSVAMP
                     VEPRIQDNAAAGEKYVAIGFGSVNDKGDQWGRRRILEDLTIDCVGTCNSFFVNNDNEW
                     EGDKGVCQGDSGGPALDDQGRVIGVVSRGSFNCSMPIYGSVYAWRDFLQQHALEAAAA
                     GGYDPPPWAGGPQPDAGTEPPDSGTDSAAPDAPIESEPEPQPTPLADLGQRCVNHVDC
                     ATGLCVYETNLNLYCSVPCTIAFPNCPDGYTCDPSLGACFKDGGFGAKCTVGSDCRSG
                     ICVEDSHGTYCTEPCTSNDQCPFSSTCAQGACFLPNPKPTPSPNKNSGCNISLKDDPT
                     KPIPWALGLGIALALLSRRRRS"
BASE COUNT          437 a          671 c          524 g          418 t
ORIGIN      
        1 ttgcgggttt atagctatcg gcaccgaccg tacgccgaaa tatcccacgg caaacgaacc
       61 cgcaaacaaa tcatggtccg tattgccggt ggcggtagct ttcgaaaacc cagacgtgct
      121 tacctcggca gaagcttctt gagccatcga agaccgactc gagaccaagg ccaacgcggc
      181 gaccataacc gccacgccgg ttctaccaag ccaacgattc cgcttccaca tggttcattt
      241 cccgttgttc ggcgtattga ctccctacga ccccaaggcc aacgcctcag gtcttcgccg
      301 agcatcttgc ggtgtaggcc gcgcaacgtc aagaaatcct ttatcgaaga atctccatct
      361 gcttcgatcc cttcggcccg cacggtgcta tgctgacact tgcctgtcgg tcttattccc
      421 gaggtcgttc atgcttcgac gcattgtctt gttcgccaca cctgccctct tcacgctcct
      481 cgcctgtcaa ggtcccacgc aaccgcaatc ggatgccatc cgatacctcg gacaaggcat
      541 catgggcggc gcggatgacc ccgatcatcc cgccatcgtg ggcatcatca gcattcaggg
      601 catgggggcg ggattgtgtt ccggcaccct catcgctcca aacctagtgc tcaccgcacg
      661 acattgcgtc gccgaaaccc aagaacaagt ctcctgtaca tcctccacgt tcggaaaaac
      721 cttcggcgca agcagcttct ttatcaccac cgaatggcag ggaccggaga tcgcctacca
      781 aacctacgaa ctgaaaggaa attggtttca agccgccgaa atcatcgttt cagacgacaa
      841 ccacgtatgc ggtaacgata tcgcgctcat tcggctcgcc accaatgttc caaactccgt
      901 cgccatgccg gtcgaaccgc gcatccagga caacgcagcc gcgggggaaa aatacgtggc
      961 gattggcttc ggatccgtca acgacaaggg tgatcaatgg gggcggcgac gtatcctcga
     1021 ggacttgacc atcgattgcg tgggcacgtg taattcgttt ttcgtgaaca acgacaacga
     1081 atgggaaggc gacaaaggcg tttgccaggg ggattcggga gggccagcgc tcgacgatca
     1141 ggggcgagtc atcggtgtgg tgtctcgcgg ttcgttcaat tgcagcatgc ccatctacgg
     1201 cagtgtatac gcttggcggg acttcctgca gcaacacgct ttggaagcag ccgcggcggg
     1261 aggatacgac ccaccaccat gggccggtgg tccacaaccc gacgctggca ccgaaccgcc
     1321 cgatagcgga accgacagcg ccgcacccga tgcgcccatc gaatcggaac ccgagccgca
     1381 acccactcct ctcgccgacc ttggacaaag gtgcgtcaac catgtcgatt gcgccacggg
     1441 cttgtgcgtg tacgaaacca accttaattt gtactgttct gtgccttgta cgatcgcctt
     1501 tcccaactgt ccggatgggt atacctgcga cccaagtctc ggcgcgtgtt tcaaagacgg
     1561 tggtttcggg gccaaatgca ccgtgggctc cgactgtcga agcgggatct gtgtcgaaga
     1621 cagtcatggg acctattgca cggagccctg caccagcaac gaccaatgcc cgttttcttc
     1681 cacgtgcgcg caaggagcat gtttcttacc caatcccaaa ccaacccctt cgcccaacaa
     1741 aaatagcggc tgcaacataa gcctcaaaga tgaccccacc aaacccattc catgggcgct
     1801 cggcctgggt atcgctctcg ctttgctctc tcggcgacgt cgctcgtagc cttccagctc
     1861 ctttccccac gccatgagcc cgaccttgtc tcgtcgcgcc tgcttgcttg ccgctgcatt
     1921 cgctttgtct tgccgttctt ccaacccatc tcgccccgga gacggccaac gaatcgtatc
     1981 gctgactccc aacaccaccg aaacggtcta cgcccttggc gcgggaaacc gaatggtcgg
     2041 tcgctcccgt
//
LOCUS       DAOSOR010000783         2046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_54801_length_2046_cov_3.918132, whole genome shotgun sequence.
ACCESSION   DAOSOR010000783 DAOSOR010000000
VERSION     DAOSOR010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54801_length_2046_cov_3.918132"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2046)
                     /locus_tag="PK710_24855"
     CDS             complement(<1..>2046)
                     /locus_tag="PK710_24855"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOT13036.1"
                     /translation="DCDGGVCLPSGVCCESADRACGDVCCQAGDACLFGACVTPGDIC
                     FTPADCPDGHYCEPALGSSSSPDAGSPDAAPPEDGGPICTQDTPPSGRCVPAPIVCDD
                     KGQPPGCVEPCEYKPKPGALRTVVKWQWGMEPKPTEYQDFADVWATPAVARIYDANCD
                     GKVDLADPPNIVFVSGNAKRTCCSCSGDAVSTCLTGVLRMLDGRNGREIWSLPKASAT
                     STGFAGMSVAVGDVDGDQLLDIIAMTGEGHIAVINHDGTVKAISTEKADGHDAGTFGW
                     GGGIAIGDMDNDGRVEIAYGRTLFSYNDKGQVERLWVGTAGSGSGLAQALSHFVDLDG
                     DGTLELLAGRTAYRKDGSMLWNNSEANGFTAVGDFDGDGLPDVVVVHNGTIRILKGLD
                     GTILLGPLTLPGTGSGGPPTVADFDGDGKPEIGVAQQNLYSMLKPDFTNNQIKVVWSQ
                     TNHDMSSSVTGSSVFDFQGDGKAEVIYNDECYLWVYDGADGSILYTDFTQSFTATESS
                     IVADVDGDGHAELVKIANGANPNTWSCAHHDEPGKIVAPHTEPFPVWQPAPSGDYRGI
                     TVLGDVENAWVGTRTLWNQHAYEVTNICDPRDSACESGSYYGQIPKNQKPNWKQPWLN
                     NFRQNVQDKGVFDAPDATVSLSVSCRMPVPLTVSVRNMGLSGLPAGVEVGLFRVASP"
BASE COUNT          432 a          591 c          575 g          448 t
ORIGIN      
        1 cgggagaggc gacgcggaac aatccgactt cgactcccgc gggaaggcca gacagcccca
       61 tgttacgaac gcttacggta agggggacgg gcattcggca agaaacgctc aacgatacgg
      121 tagcgtccgg cgcatcgaaa acgcctttgt cttgcacgtt ttgtcggaaa ttattgagcc
      181 agggttgttt ccaattgggc ttctggttct ttgggatttg tccgtaatag gaaccacttt
      241 cacaagcgct atcccttgga tcacagatat tggtcacctc atacgcgtgt tgattccaga
      301 gagtacgtgt tccgacccat gcgttttcga catcccccaa gacggtaatt ccccgatagt
      361 cgccgcttgg agcgggttgc cagacgggga aaggctctgt atggggggcc acgatttttc
      421 ctggctcatc gtggtgagcg caggaccaag tgttgggatt cgctccattg gctattttga
      481 cgagttccgc atgtccatcg ccatcgacgt ccgccacgat ggacgactcg gtagcggtga
      541 acgattgcgt aaagtccgta tacaaaatgg atccgtcggc gccgtcgtac acccaaagat
      601 agcactcgtc gttgtagatg acttccgcct tgccatctcc ttgaaagtca aaaacgctcg
      661 atccggtgac ggaagaactc atgtcgtggt tggtttgaga ccaaaccact ttgatttggt
      721 tgttggtgaa atcgggtttg agcatcgaat acaggttttg ttgcgctaca ccaatctccg
      781 gtttcccatc tccatcgaag tcggccacgg tcggcgggcc tccgcttccc gttccgggta
      841 gtgtcagcgg gccaagcaag atggttccat caagaccttt gagaatacga atcgtgccgt
      901 tgtgaaccac cacgacatcg ggcaaaccat cgccatcgaa gtctcccacg gcggtgaacc
      961 cgttggcttc ggaattgttc cacagcatcg acccatcttt gcgataggcc gttcgccccg
     1021 cgagaagttc aagcgtgcca tcgccatcga ggtcgacgaa atgcgagagg gcctgggcaa
     1081 gtccgctgcc ggagcctgcg gttcctaccc acaagcgttc gacttgtccc ttgtcgttgt
     1141 agctgaaaag tgttctgcca taagcgattt ccacgcgtcc gtcattgtcc atgtcaccga
     1201 tggcgatgcc accgccccat ccgaacgtgc cggcgtcgtg gccatccgct ttttcggtgc
     1261 tgatggcctt gacagtccca tcgtgattga tcacggcgat atggccctcg cctgtcatgg
     1321 caatgatatc gagaagctgg tccccatcga cgtcacccac cgccacactc atcccggcaa
     1381 accccgtgct tgtcgctgaa gccttgggca aggaccagat ctcgcgtcca ttccggccat
     1441 cgagcatccg aagcacaccg gtgaggcaag tggaaacggc atcgccggag caactacaac
     1501 acgtgcgttt cgcgttaccg gaaacgaata cgatgttggg aggatccgcc aagtccactt
     1561 tgccatcgca attcgcatcg tagatacgag ccacggcggg agtcgcccaa acatcggcaa
     1621 agtcttgata ttccgtgggt tttggttcca ttccccattg ccacttcacc accgtacgaa
     1681 gcgctcctgg tttcggtttg tattcgcagg gttcgacaca acccgggggt tggcctttgt
     1741 catcgcagac gatgggagcg gggacgcatc gacctgaggg gggcgtatct tgggtgcaga
     1801 taggaccgcc atcttccggc ggggcggcgt cggggcttcc cgcatcgggg gaagaggaag
     1861 aaccaagggc gggctcacaa taatgtccgt ctgggcaatc ggctggtgtg aaacagatat
     1921 ccccgggggt aacgcatgct ccaaaaaggc atgcatcgcc agcctggcag caaacgtctc
     1981 cgcacgcacg atcggccgat tcacaacaga cgccgcttgg caaacaaacg ccgccgtcgc
     2041 aatccg
//
LOCUS       DAOSOR010000784         2045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_54835_length_2045_cov_3.386432, whole genome shotgun sequence.
ACCESSION   DAOSOR010000784 DAOSOR010000000
VERSION     DAOSOR010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54835_length_2045_cov_3.386432"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2030
                     /locus_tag="PK710_24860"
     CDS             <1..2030
                     /locus_tag="PK710_24860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HOT13037.1"
                     /translation="PQQLYLPVISDPIYGYPAVNVEAMERTPTSLLHWMRAIIAVRRR
                     HRAFGRGGLHLLYPSNHHILAYLRHHDEELLLCVFNLSASAQAVELDLHEHVGRYPVE
                     LLGGSTFPRIGQLPYLLTLPAYGYYWFRLSSPHQPPLPMPDRPDLPFLVIGRQARVDD
                     VLGPVFHDILGDFLHRQRWFQAKGSVILAIQATLDRVWETGPGEWLWAIVRVEVQNQA
                     PQRYSLPLAVLWDEQAESVKQSAPARILAKIRRGPRIGVVCDAYLDPQFALQMSSAFD
                     RAEHLTLAGGTLRFLATAAYQSPVHLQPAQVEPLDVEQSHTSVRLGSLQIYKSYRRLQ
                     RRGRSEVEIGRYLTESSPFPNTAPVHGHVELSDIDGENQTIGILVGYIPNEGDAWSYT
                     SNYLEHYVEARLADEEGHGKPTERHGAYAPFAKNLGKRTGQLHLALSRPTADPAFSPR
                     PMTGEDVFRWASFVRQDAIRTVDTVEASLHRLDESALPYAQRLVERKRELLERLEREA
                     PHPCEGLCCRFHGDFHLGQVLLSRGDAYIIDFEGEMARPEGTLEEKHSPLRDVAGMLR
                     SFNYAAIVALAEISASLGHAVQLEGIVRAWEQEARQSYLEGYRAVTRGRGLVPDNQEA
                     FSYLLELFLFEKALYEINYELNSRPRWVLAPLRGVLALLDHPGFPQEPSSK"
BASE COUNT          415 a          576 c          572 g          482 t
ORIGIN      
        1 acccccaaca gctttacctg ccagtgatat cggatccgat ttacggctat cccgcggtga
       61 atgtcgaagc gatggagcga acgccaacct ccttgctcca ttggatgcgg gcgatcatcg
      121 ccgtccgtag gcgtcatcga gccttcgggc ggggtggttt gcacctgctt tatccatcca
      181 atcaccatat cctggcctat cttcggcacc acgacgaaga actgctgtta tgcgtgttca
      241 atctttctgc ttcagcccag gcggtcgaat tggaccttca tgagcatgtg gggcggtatc
      301 cggtggaatt gctcggaggt tccaccttcc ctcgcattgg gcaactgccg tatctgctca
      361 ccttacccgc ctatggctac tattggtttc ggctttcctc gccgcatcaa ccgcctctgc
      421 ccatgcccga cagacccgat ttaccctttt tggtcatcgg aaggcaggcc cgggtggacg
      481 acgtcctcgg ccctgttttc catgatatcc ttggggattt tcttcatcga caacgctggt
      541 ttcaggccaa ggggagcgtt atcctggcaa tccaagctac cctcgatcga gtgtgggaaa
      601 caggacccgg cgaatggctc tgggcgattg tccgtgtcga agtgcagaac caggctcccc
      661 aacgatattc attacctctc gccgtattgt gggacgagca agcggaatcg gtgaaacaat
      721 cggcgcccgc ccgaattctg gccaaaattc gcagaggtcc aagaatcgga gtggtttgtg
      781 atgcctacct ggacccgcaa tttgccttgc aaatgtcgag tgctttcgat cgagccgagc
      841 atcttaccct cgcggggggc acactccgct tccttgccac tgcagcttac caatcacccg
      901 tccacctgca gccagcgcag gtcgagcctc tcgatgtcga acaaagccat acctccgtac
      961 gcctcggctc cttgcaaatc tacaagagtt accgtaggtt acagcgacgt ggccgcagtg
     1021 aggtggaaat tggcaggtat ctgacggaat cctctccttt tcccaacacc gcaccggttc
     1081 atggtcatgt ggagctttca gatatcgatg gggaaaacca gaccatcggg attctggtgg
     1141 gctacattcc caatgaagga gacgcctgga gttatacgtc gaattatctc gaacactatg
     1201 tggaagcgcg attggccgac gaagagggtc atggcaaacc gaccgagcgg catggggcct
     1261 acgcgccatt cgccaaaaac cttgggaaaa ggacgggaca actgcatctg gccttgtctc
     1321 gacccacggc ggatcccgcg ttttcgcctc gacccatgac cggcgaggac gtttttcgtt
     1381 gggcttcttt cgtgcggcag gatgcgattc gaaccgtgga tacggtggag gcatcgctac
     1441 atcgtcttga cgaaagcgcg ctgccctatg cacaacgttt ggtggaaagg aagcgggagc
     1501 tgttggagcg tctcgaacgg gaggcgccgc atccctgtga aggcctatgt tgtcgttttc
     1561 atggggattt tcaccttggc caggttctcc tttcccgggg ggatgcttat atcatcgact
     1621 tcgaggggga gatggctcga cccgagggaa ctctcgaaga gaagcattca ccgctgcgcg
     1681 acgtggcagg aatgcttcgc tccttcaatt atgcggccat cgtggcattg gcagagatat
     1741 cggcaagtct cggacatgcg gtgcaactcg aagggattgt ccgagcgtgg gagcaagagg
     1801 ccaggcaatc gtatttggag ggttatcgag ccgtgacccg gggccgcggg ttggtgcctg
     1861 acaaccaaga ggcattttcc tatcttctcg aattgttttt gttcgaaaaa gctctttatg
     1921 aaatcaatta cgagctgaac agtcgtcctc gctgggttct ggcaccgctt cgcggtgtgc
     1981 tcgccctgct tgaccatcct ggctttcccc aagagccatc ttcgaaatga ttgcaggatt
     2041 accta
//
LOCUS       DAOSOR010000785         2038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_55110_length_2038_cov_3.163893, whole genome shotgun sequence.
ACCESSION   DAOSOR010000785 DAOSOR010000000
VERSION     DAOSOR010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55110_length_2038_cov_3.163893"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..440)
                     /locus_tag="PK710_24865"
     CDS             complement(126..440)
                     /locus_tag="PK710_24865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT13038.1"
                     /translation="MDKRLAMLEDLTRSGKADAFAWYALALEYKSKARFDDAMQTFST
                     LREKEPGYIPMYLMAGSMLIELGREQEARAWLTDGIQRAEAANNAHARDEMEALLDSI
                     SS"
     gene            complement(492..1829)
                     /locus_tag="PK710_24870"
     CDS             complement(492..1829)
                     /locus_tag="PK710_24870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13039.1"
                     /translation="MTDRIEITKMDPIVREGLEVLRRSGANKALRATIQAVAAVADET
                     TEESVAYLIACYQGAKVFAAAGDMDAAITLMNTVPRQVAERPENALILSTLALEVGEG
                     MRRLGAVDTAEKIVGLGHELRRHQFGRFHPRTGLSSLYAARICMDQRRWSDAHKYYEA
                     SVVGLGPYHPFNAAAYAERAFAVQMIAPNARLFPEFVMSAPRPYWRRLVNFMAGTTLQ
                     LPLDLRMAVLLNVSCEMEETLEDSGDLSRPLLVTAYQHACTSNDERGEELEEVLQERG
                     WCDDQVRMPPTLPEAGSDTESFWTAPDRDDDSRPMDALAISFKLFEGAAEASAGRKSS
                     AIDAFERVRDASDEDTIEHWAARGCLQLLDRGWKLRHNQFKPEIRAIEALALSKLPKT
                     TQTKLRGIELSVRKGSLDVSFLGAELTEAQQKRAAQAVQSALTWITDRSEEAS"
BASE COUNT          439 a          608 c          550 g          441 t
ORIGIN      
        1 ccctcgcgcg tatcgctcac gtcatcgtct cctgccacca gagcttttag cgccccaaac
       61 cccacgaatc ccgcggaccg aatcgtttcc cgggattata gaaattcagc taataaaaca
      121 ataggttagg aggatatcga gtcgagaagg gcctccattt catcgcgggc atgcgcgttg
      181 ttcgccgcct ccgcccgttg gataccgtcg gtcaaccaag cacgggcttc ctgctcccgg
      241 ccgagttcga tcagcatgga tccagccatc agatacatgg gaatatatcc aggctccttt
      301 tcgcgtaatg tcgaaaaagt ctgcatggcg tcgtcgaatc gagctttgga tttgtactcc
      361 aacgcgagag cataccacgc aaacgcatcc gcttttccgg agcgagtgag gtcctcgagc
      421 atggcaagac gcttatccat gggcgaaccg tggcattgct cggagcgtat ggccacaaca
      481 attccggtga gtcatgaagc ttcttcgctt cggtcggtaa tccatgtgag ggctgattga
      541 accgcttggg ccgctcgctt ctgctgcgct tcggtcagtt cagcgccaag gaacgagaca
      601 tcgagggaac cttttcgaac cgataattcg atgccgcgca acttcgtttg cgttgttttg
      661 ggaagcttgg ataacgcaag cgcttcgatg gcacgtattt ccggcttgaa ttggttgtgc
      721 cggagcttcc acccgcggtc gagaagctga agacagcccc gggccgccca atgctcgatg
      781 gtatcctcgt cgctcgcatc gcgtacgcgc tcaaaagcat cgatggccga ggattttcgc
      841 cccgccgatg cctccgcggc tccctcgaat agcttgaacg atatagctaa cgcgtccatc
      901 ggtcgactat cgtcatcgcg atcaggagcg gtccaaaatg actccgtatc cgatccagct
      961 tccggaagag tcggtggcat gcgcacttgg tcgtcacacc aaccccgctc ttgcaacact
     1021 tcttcgagtt cctcaccacg ttcatcgttc gaagtacagg cgtgttggta tgcggtcacc
     1081 aacaaaggcc gcgagagatc gccgctgtct tcgagcgttt cttccatctc gcaactcacg
     1141 ttgagcaaca ccgccatacg taagtcgagg ggaagctgaa gggtggtgcc ggccatgaaa
     1201 ttcaccagtc ttcgccaata cggccgcggt gcggacatca caaattcggg aaatagccgt
     1261 gcattcggag cgatcatctg taccgcaaaa gcgcgctccg cgtaggctgc cgcgttgaaa
     1321 gggtgataag gccccaaccc cacgacgctg gcttcataat acttgtgagc gtccgaccag
     1381 cgccgctgat ccatgcagat gcgcgccgcg tagagcgaag acaaccccgt tcgtggatga
     1441 aagcggccaa attgatgtcg tcgcaactca tggccgagcc ccacgatctt ttccgctgta
     1501 tctaccgctc caagccgacg cattccctcg ccgacctcga gggcgagcgt ggatagtatc
     1561 aacgcgtttt cgggtcgctc cgctacctgt ctgggtaccg tattcatcaa ggtaatcgcc
     1621 gcatccatgt ctcccgcggc ggcaaaaacc ttcgcccctt ggtagcaggc gatgaggtac
     1681 gccacggatt cctccgtcgt ttcatccgcc acggcagcca cagcttgaat cgtggcccga
     1741 agcgccttgt tcgcgccact tcgacgcaag acttcaagcc cttctcgtac gatggggtcc
     1801 atcttggtga tctcgattcg atcggtcatg agatgcgttc agggttgtag gacgtccatc
     1861 cgacgccgaa gaaatactct cggaaacgag gggttggaga tgagtcgctg ccatggtggc
     1921 cggattcgaa cagacaacac gctggaattc atatcaacca ccagataagg ataccgaacc
     1981 gttttcgcgt aacgacaaag atgccgcctg ctcggatgtg tcagaagacg aagggacc
//
LOCUS       DAOSOR010000786         2015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_55990_length_2015_cov_4.262245, whole genome shotgun sequence.
ACCESSION   DAOSOR010000786 DAOSOR010000000
VERSION     DAOSOR010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55990_length_2015_cov_4.262245"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PK710_24875"
     CDS             <1..268
                     /locus_tag="PK710_24875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13040.1"
                     /translation="FDMVPVSDSSSLEVSWPGNTYTFAKNADGCECHNRFEAPAGKYR
                     IEVPVYLTNELYPSTPDYTAVVDFTLPAPSGVVTVDLTEAYPED"
     gene            complement(501..1745)
                     /locus_tag="PK710_24880"
     CDS             complement(501..1745)
                     /locus_tag="PK710_24880"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOT13041.1"
                     /translation="MDRVFPCLNESGSQPGDEPQQICSVASRKRVGAFGEILRKQILI
                     PFVVLAFSVGCAGSSSESQGDPQADSGASSLDTGAVEQDANFVPDASDDQVDGGSGED
                     AEIGEDAEIEEAGDEQGVEVDGGEPSEPDAAVPPPTHKQHILRIFHWNIAGGKENGCK
                     PGPISEAVLKFVKDHKVDFVGLNEVCPKQHDAIEKALRAEWKVPNGTQFAAYKGDELA
                     TVVGNSIFARLGFVPDSIEREELGADQYGKRFLICAKILGFPHLRQCATHLSPNDDKA
                     GNQLVKMLGVIEKYWEKQNDTVFFTGDLNLRADAKALDIVYSAAVNTKNNSNNKGQYR
                     EVDDNDPQHCLGYGERTLPNTTGGPCKQGGKIDFIFIRQNHIVAGDYDGDTFNIPKTC
                     NGKVCSDHRPVFGRARVQIALD"
BASE COUNT          456 a          575 c          513 g          471 t
ORIGIN      
        1 gttcgacatg gttcccgtaa gcgacagctc gagcctcgag gtttcctggc ccggcaacac
       61 gtatacattt gccaaaaacg ccgacggctg cgaatgccac aataggttcg aagcgcccgc
      121 cggcaaatat cgcatcgaag taccggtata tctcaccaac gaactctatc cctctacgcc
      181 cgactatacc gctgtcgtgg atttcacgct gccggcgcca tccggcgtgg tcaccgtgga
      241 cttgacggaa gcctaccccg aagactgaca ggggcctata agccacggca tcattggcaa
      301 ttgtcgaggg gttcgaggct tttcggtcga aaaagtcggt gcctgaacca gccccttcct
      361 tttccctcga gcctttcctt cacggccaag acccacgaga aacggtcact ccaagaattc
      421 gccgctcgta tccctttgcc attcgggaaa aatgagggga tctatcagga tccgggtgat
      481 tcggatcagg gaaagatgcg tcaatcaagc gcgatctgca cccgggcgcg accaaagacg
      541 ggacgatggt cggagcatac cttgccgttg caggtcttgg ggatgttgaa ggtgtctccg
      601 tcgtaatcgc cagcgacgat atgattctgc cgtataaaaa tgaaatcgat ctttccgcct
      661 tgtttgcagg ggccgcccgt ggtgttcggc agggtccgtt cgccatagcc caggcaatgt
      721 tgcggatcgt tatcatcgac ttcccgatat tgccccttgt tatttgagtt gtttttggtg
      781 ttgacggcag cgctatagac tatatccaat gctttggcat ccgctctcag gttgagatcg
      841 ccggtaaaaa acacggtatc attctgtttt tcccagtact tttcgatgac tcccagcatc
      901 ttgaccagtt ggtttccggc tttatcgtcg ttcgggctga gatgggtcgc gcattgccga
      961 aggtgcggaa agcccagaat ttttgcacaa atcaagaaac gtttgccata ctgatcagcg
     1021 ccgagttcct cgcgttcgat cgaatcaggc acaaatccga gacgagcaaa gatcgagttt
     1081 cccacgaccg tggccagttc atctccctta taagcggcga attgcgttcc attcggcacc
     1141 ttccattcgg cgcgaagagc tttttcgatg gcgtcgtgct gttttgggca tacttcattc
     1201 aggcccacga aatccacctt atggtccttc acaaacttga gcaccgcttc ggaaatcggc
     1261 cccggcttac agccattctc tttgccgcca gcgatattcc agtggaaaat ccgcaagata
     1321 tgttgtttgt gggtcggcgg aggcacggcg gcatcgggct cggaaggctc acccccatcg
     1381 acttcgacac cctgctcatc gccagcttcc tcaatctcgg catcttcccc aatctcggca
     1441 tcttccccag aaccgccgtc aacctgatcg tcgctcgcat caggaacaaa gttcgcatcc
     1501 tgttcgaccg cgcccgtatc gaggctcgag gcaccgctgt cggcttgcgg atcgccctgc
     1561 gattcggagg aggagcccgc acaacccacc gaaaaagcaa gcaccacaaa gggaatcagt
     1621 atctgctttc gcaaaatttc tccaaacgcc cccacccgct ttcgtgaggc gacagagcaa
     1681 atctgttgcg gttcatcccc aggctggcta cctgattcgt tcagacacgg aaaaacgcga
     1741 tccatagcac ctcccaatgg gcgagagaat agcaccccat cgtcggacgg tcaacggcat
     1801 gggataaatt tgatgatact ttatggagcc caattcggtt gccatgggcg gcaaggtttt
     1861 ccacttggac ataaagaggc aacacccgat tcgaaaaagc tcgttgccct tacccccatc
     1921 cttgaggaag ggattgtaat cgtgtaactt attgataaaa tagcatgggg gcgtactttg
     1981 ggaagaaaga acgcaagata gttggccaag ttttt
//
LOCUS       DAOSOR010000787         2011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_56143_length_2011_cov_3.717791, whole genome shotgun sequence.
ACCESSION   DAOSOR010000787 DAOSOR010000000
VERSION     DAOSOR010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56143_length_2011_cov_3.717791"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PK710_24885"
     CDS             <1..820
                     /locus_tag="PK710_24885"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOT13042.1"
                     /translation="EADSPQRSLPSGFAQDSTCMVELPQFEGPLDLLLHLITKHELDI
                     LDIPIAFITDKYLEYLSQLKAWSIDVASEYLVMAATLAHIKSKMLLPSPPPGQDDVLE
                     EEQEDPRAELVRRLLEYQKYKAAAVDLGDRALFGADVFGRGEMEPRDGGPAPLAPVGV
                     FALIDAFQRIAERRKIRIDHEIDLERISITERIGELSELLRTRGPCTIDDLLPDRMGT
                     SELIITFLALLEMTRLRMTRLQQSHPLAPLHVTLAVSEQQEQKSGVGPSNDNEA"
     gene            817..1824
                     /gene="scpB"
                     /locus_tag="PK710_24890"
     CDS             817..1824
                     /gene="scpB"
                     /locus_tag="PK710_24890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOT13043.1"
                     /translation="MSDPTHPPIGSSGGVSDVSSTPPKDEREAPILDSHENEPPPFDM
                     PDSQPPQRSASVMRSDEADAEPTSERNYPPADVEDDVVRSHLKGLIEALVFASDAPLP
                     APEIAKTAKADRKLVMELLRELQEEYLSRGIRLVEVAGGFAFRTASAYAPFVRDVTAQ
                     RPVKLTRAQLETLAIVAYRQPLTRPEIDDVRGVDSGPVLKMLLDRGLVRILGKRDEPG
                     RPLIYGTTSTFLSFFNLRSLKDLPSLREFTELTDDSKRAFEKALGEEPPEQLDFGEAV
                     WTNPTESGDELVADEHLSQIGHESNTSHSAQEPDPMGTDPHVSDEDDSDDEAPRSRAS
                     R"
BASE COUNT          419 a          611 c          570 g          411 t
ORIGIN      
        1 tgaggcggat tcgccgcagc gttccttacc ttccggattt gcgcaagatt cgacctgcat
       61 ggtcgagctt ccgcagttcg aagggccatt ggatcttctg cttcatctca tcaccaaaca
      121 tgaactcgac atcctcgata tccccatcgc cttcatcacc gacaagtacc tcgagtacct
      181 gtctcagttg aaagcatggt cgatcgacgt cgctagcgag tatctcgtca tggcggccac
      241 cttggcgcat atcaagtcca agatgttgtt accttctcct ccaccggggc aagacgatgt
      301 gttggaagag gaacaggaag accctcgggc ggagttggtg cgacgcttgt tggagtatca
      361 gaaatacaaa gcggcagcgg tggatttggg ggatcgtgcg ttgtttggcg cggatgtttt
      421 tggacgtgga gagatggagc ccagagatgg tggaccagct cccctggcac cagttggcgt
      481 ttttgctctg atcgatgctt ttcagcgcat cgcagagcga cgaaaaatcc gtatcgatca
      541 cgaaatcgat ctggaacgca tcagcatcac cgagcgcatc ggtgaattgt ctgagcttct
      601 tcggactcga gggccatgca cgattgacga ccttcttccc gatcggatgg gaaccagcga
      661 acttatcatc acttttcttg cgttgctcga aatgacacgt cttcgcatga ctcgattgca
      721 gcaaagccac ccgcttgccc ctcttcacgt aacgcttgca gtctccgagc aacaggagca
      781 aaagtcgggc gttggaccgt ccaacgacaa cgaggcatga gcgatcccac gcatcctccc
      841 atcggctcca gcgggggagt ctccgacgtt tcctcgacac ctcccaagga cgagagagag
      901 gcgccgatat tggattcgca cgaaaacgag cctcccccct tcgacatgcc ggactcccag
      961 ccgccgcaac gttcggcttc cgtcatgcga tcggatgagg cagacgcgga gccaacatcg
     1021 gagcggaatt atcccccagc cgatgtcgag gacgacgtgg tgcgttctca tctcaaaggc
     1081 ttgatcgaag cactcgtttt cgcgagtgat gctccactcc ctgccccgga aatcgccaaa
     1141 acggccaaag cggaccggaa actcgtcatg gaattgctcc gtgagttgca agaggaatac
     1201 ctgtcgcgcg gtatccgatt ggtggaggtc gccgggggat tcgcgtttcg aaccgcttcc
     1261 gcttatgctc ccttcgttcg cgatgtcaca gcacagcgtc ccgtgaaatt gacacgggcc
     1321 cagctcgaga cgctcgccat cgtggcctat cgccaaccgt tgacgcgacc tgaaatcgac
     1381 gatgtgcgcg gcgtggattc cggacctgtc ctgaaaatgc tcttggatcg cggcttggtg
     1441 cgtatccttg gcaaacgcga tgaaccaggt cggcccttga tctatggcac gaccagcacg
     1501 tttttgtcgt ttttcaatct caggagtctc aaagatcttc catctttacg cgagtttacg
     1561 gaactcacgg acgactccaa acgagctttc gaaaaggcgt tgggggaaga accgcccgag
     1621 caactcgact tcggcgaggc cgtatggacc aatcccacgg agtccggtga tgagctggtc
     1681 gccgacgaac atttgagcca gattggtcac gagagcaaca ccagccactc cgctcaagag
     1741 cctgacccaa tgggaacaga cccacacgtt tcagacgaag acgactcgga cgacgaggct
     1801 ccacgctctc gagcgtcgcg gtgacgtcgc cgtcgactcc gaggggcgcg ggtggctgcg
     1861 cgtcggcaga cgccctctcg acggcccgct gccggacgtc accctcggtg atcaggccgt
     1921 cggggccgga tcccacgatc ccgtcgagcg ccaccccgag ctcccctgcc acgcgccgcg
     1981 ccctggggct cgcagcgtct gccgagaggc g
//
LOCUS       DAOSOR010000788         2003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_56420_length_2003_cov_4.376283, whole genome shotgun sequence.
ACCESSION   DAOSOR010000788 DAOSOR010000000
VERSION     DAOSOR010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56420_length_2003_cov_4.376283"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PK710_24895"
     CDS             complement(<1..618)
                     /locus_tag="PK710_24895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13044.1"
                     /translation="MRLFSRPVRMSRKPTKTTRTGHYALLFARALVVGIFLSWGSRVQ
                     AADTSETFAVGVTDVEWYSGVDRVEGGAKDRTVFADMMLGIGLLHRLSAYTGVVMQGT
                     GAFTEGQSSQYMGLLGTPLDTDHCDLDLQLDVSSGGPSQQFELRPAFELNVDGDPQMR
                     SWGVYVRAPFPVYGRKMGNGRTHATFHLELTVGAYAHLIDGHQVLI"
     gene            1029..1283
                     /locus_tag="PK710_24900"
     CDS             1029..1283
                     /locus_tag="PK710_24900"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HOT13045.1"
                     /translation="MAIVSLVAGIVSWFMCPFIGGIVALICGFMARKDIAKDKTKWDG
                     DSLALAGMIVGGLNVIGYLLVGILYLLMFVGAFSLAIFGS"
     gene            complement(1258..>2003)
                     /locus_tag="PK710_24905"
     CDS             complement(1258..>2003)
                     /locus_tag="PK710_24905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13046.1"
                     /translation="NPLHTCHVDGRTCEIADAIASPSLLRGSRSAARLHSAFEFVTDV
                     LPCAFCREAEASVDRLRGVRFGKHERNILQYSPGPTSPSGAILDPDLSTHSDRETYLR
                     AVRKLSRLGLLEVGHRLVRVQTGSRRKDGTAVTRAYMHRTLQQTALGALVVEAYRSEM
                     EAGKAIRWEKQLDGLRQRARSSLNELLVEFGVSLETRVETLALESQAGGVDAKRARET
                     RYLVQPLLDALKSTVRWNDDEVKNQRSRG"
BASE COUNT          465 a          616 c          519 g          403 t
ORIGIN      
        1 gatcaacact tgatgcccat cgatcaaatg agcatacgca ccgaccgtca attcgagatg
       61 aaaggtggca tgcgttcgac catttcccat tttgcgcccg tagacaggaa atggagcacg
      121 tacatacacc ccccaagaac gcatttgtgg atccccgtcg acattgagtt cgaaagcggg
      181 acgcaattcg aattgttgcg aaggtccccc tgaggatacg tcgagctgga gatccaaatc
      241 gcaatggtcg gtatccaagg gggtgcccaa aaggcccatg tattgcgacg attggccttc
      301 ggtgaaggcg ccggttcctt gcatgacaac cccggtgtac gcggaaagtc ggtgcaatag
      361 gccgatgccg agcatcatgt cggcgaatac cgtacgatcc ttcgcgccgc cctccaccct
      421 atccacaccg ctataccact cgacatccgt gacacccaca gcgaacgtct cgctggtatc
      481 cgccgcttgg actcgactgc cccacgatag aaaaataccg accacgagag cgcgggcaaa
      541 gagcaaggca taatgtcctg ttcgcgttgt tttcgtgggc tttcggctca tccgaacggg
      601 gcgagagaag agacgcatgt cgtacttggg gtctagctaa aagtgataga cttctctacc
      661 aatataaaga gtatctttca agagaatatg ggctggagac tccatctgcc tatctgaaaa
      721 ctcgtgggag gagggcaaag gtcaggcgcg aaaagccgtg gaagcttcat agaagcaccg
      781 gcacacgcga gggaagcaag ccggccatga gccccgcgga taagtgggtt gacaccctca
      841 ccccaacgaa cgaccatctt ccgatgcaac aacaaccagg tggttttggt cctcctccgg
      901 caggttttgg caatcctccc cctgcaggtt ttggagcctc tcccagtccc tacggcggcc
      961 cacccgcagg cggattcgga cccggtcaat tccctgctcc acccccgcta cctcgtcaaa
     1021 cccataccat ggcgatcgtt tcgctggtcg ccggcatcgt aagctggttc atgtgccctt
     1081 tcatcggcgg gattgtcgcg ctcatctgcg gcttcatggc acgaaaagat atcgctaaag
     1141 ataaaaccaa gtgggatggc gattccctcg ccctcgccgg catgatcgtt ggcggactca
     1201 acgttatcgg ttatctcttg gttggaatct tgtacctgtt gatgttcgtg ggcgcattca
     1261 gcctcgcgat ctttggttct tgacctcatc gtcattccaa cgaaccgtgg acttcaacgc
     1321 atcgagcagc ggctgcacga gatagcgagt ttcccgcgct cgcttggcat ccacaccgcc
     1381 cgcttgcgac tccagggcaa gcgtttcaac acgagtttcc aacgataccc caaattcgac
     1441 caacaactcg ttcaacgacg agcgagcgcg ttgacgcaac ccatcgagtt gtttctccca
     1501 acgaatggct tttcccgctt ccatctcgga ccgataggcc tccaccacca gcgcgcccaa
     1561 cgccgtctgc tgaagcgtcc gatgcatata cgcccgagtc acggccgtcc catccttgcg
     1621 ccggctgccc gtttgcacac gcaccaaacg atgacccact tcgagcagcc ctagccggga
     1681 taacttgcga accgcacgca aatacgtttc tcgatccgaa tgggtcgata agtccgggtc
     1741 aaggatggcg cccgaaggag aagtcggacc tggcgaatat tgaaggatat tgcgctcgtg
     1801 cttcccaaat cgaacgccac gaagacgatc caccgaggct tccgcctctc ggcaaaacgc
     1861 acagggcaat acatccgtca cgaattcaaa cgccgaatgc agtcgtgccg ccgaccgcga
     1921 cccccgaagc agcgaaggcg aagctatggc gtccgcaatc tcacaagttc gaccatccac
     1981 atggcaggta tgaagaggat tcg
//
LOCUS       DAOSOR010000789         1997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_56645_length_1997_cov_5.577755, whole genome shotgun sequence.
ACCESSION   DAOSOR010000789 DAOSOR010000000
VERSION     DAOSOR010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56645_length_1997_cov_5.577755"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PK710_24910"
     CDS             <1..188
                     /locus_tag="PK710_24910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA integration/recombination/inversion protein"
                     /protein_id="HOT13047.1"
                     /translation="ATFVTLALASDKSETWISDRTGHKSSEMIRRYYRKARTFAELGL
                     HALAPMDQSIPEFVAPT"
     gene            complement(259..340)
                     /locus_tag="PK710_24915"
     tRNA            complement(259..340)
                     /locus_tag="PK710_24915"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(304..306),aa:Leu,seq:tag)
     gene            684..1364
                     /locus_tag="PK710_24920"
     CDS             684..1364
                     /locus_tag="PK710_24920"
                     /inference="COORDINATES: protein motif:HMM:NF011102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOT13048.1"
                     /translation="MGKNILIFGPNGSGKGTQGAIVQKKVNVPHIESGAIFREHISGG
                     TDLGKKAKAYIDRGDLVPDDITIPMMIERLQKPDCKDGWLLDGFPRSKAQAISLAESL
                     DKAGIALDYVIEIILDREIAKLRIMGRRLCVNDNNHPNHIAFDAIKPVEKDGKLVCRV
                     CGGDLKTRADDQDEAAINKRHDIYYDEVTGTMAAIQHFKMAGGPKVISVDGSKTIGEV
                     TESILAKL"
     gene            1429..>1997
                     /locus_tag="PK710_24925"
     CDS             1429..>1997
                     /locus_tag="PK710_24925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13049.1"
                     /translation="MPRINSGLPRIVQLSLRAAESLGYRTRILDPEYGYLFEVDNGVE
                     KRCFVGGMSPLNDAVAARIAQDKYYTSMLLADRGFRVVQGVRCLRADYFRVDDFSDRA
                     GPQPGIDLANRVGYPVIVKPNRMALGRDVVAVHDESSLLRAIETVWKGDYLALVQRVA
                     WGADVRLDFLDGTFLAGYRRHPIRIVGDGR"
BASE COUNT          431 a          643 c          502 g          421 t
ORIGIN      
        1 aggccacatt cgtcaccctg gccctcgcgt cggacaagtc ggagacgtgg atctcggatc
       61 ggacgggcca caagtccagc gagatgatcc gccgctacta ccgcaaggcg cgaaccttcg
      121 ccgaactcgg gctgcatgcg ctcgcaccga tggaccagtc gattcccgag ttcgtggccc
      181 caacgtgacc ccgcttcggg ccgcggaaac ccgacgcacc cagcctaccg accgatcctc
      241 ccaagcttct ccactaagtg cacgaggagg gacttgaacc ctcacgcctc tcggcgctgg
      301 aacctaaatc cagtgcgtct gccaattccg ccactcgtgc gcggctctcc ttaccaatag
      361 ccacaccatg gccaacaaca ccaccgtgtg accttcgacg cttattgaca gcacctgcac
      421 gttttcgtca agccgcttcg actcgccttc cgtcccaatc cctttccctt catccctctg
      481 gtaccccaac gccttccacc ccattcgtcc gcatgataaa attgtataat aaatacaaat
      541 aattatatct tgacagctcc cccgcttccc catcgaatcc cccattggcg acggttccgg
      601 tttcccttca cgctccgtgc gtccacaatg ccccccgcta gcggtcgccg ttgcaccgct
      661 ccatcttcac gcggagtctg cccatgggaa aaaacatcct catctttgga ccgaatggaa
      721 gcggaaaggg aacgcaagga gccatcgtcc agaaaaaagt caatgtgccc catatcgagt
      781 ctggtgccat ctttcgtgag cacatcagcg gtggaaccga cctcggaaaa aaagccaagg
      841 cctatatcga tcgaggtgac ctcgttcccg atgacatcac catccctatg atgatcgaac
      901 gcctgcaaaa accagattgc aaggatggct ggctgctcga cggttttcct cgttccaaag
      961 cccaggccat cagcctcgcg gaaagcctcg acaaggcagg cattgcgctt gattacgtga
     1021 tcgaaatcat tctcgatcgc gagatcgcca agctcagaat catggggcgg cgcttgtgcg
     1081 tcaacgacaa caaccatccc aaccacatcg cgttcgatgc gatcaagcct gtcgagaagg
     1141 acggcaaact cgtatgtcgc gtgtgcggcg gtgatctcaa gacacgtgcc gatgatcaag
     1201 atgaagcagc catcaacaag cgtcacgaca tctattacga cgaggtgacg ggcaccatgg
     1261 cagccattca gcacttcaaa atggcaggag gccccaaagt catctccgtc gatggttcca
     1321 agaccattgg tgaggtgacc gaatccattc tcgccaagct ctgatcctcg ctggagcgct
     1381 gctacccacc cttcccccat ggtcggcgca cgagtagcat cgtgctcgat gccacgaata
     1441 aattcaggat tacctcgtat cgttcagctt tccttgcgtg ccgcggaatc cctcggttat
     1501 cgaacacgta ttctcgaccc ggaatatgga taccttttcg aggtcgataa cggggtcgaa
     1561 aaacgctgct ttgtgggggg gatgtctccc ctgaacgatg cggtcgccgc gcgaattgct
     1621 caggacaagt actacacctc catgctgctg gcagaccggg ggtttcgcgt cgtgcaaggc
     1681 gttcgctgtc ttcgcgcgga ctactttcgt gtcgacgact tctccgatcg tgccggtccc
     1741 caacctggaa tcgaccttgc caaccgtgtg ggatatcccg tcatcgtcaa gccgaatcga
     1801 atggctcttg ggcgtgatgt cgtagcggtc cacgacgaat cctcgctcct acgtgccatc
     1861 gaaaccgtgt ggaaaggtga ctatctcgcc ctcgtccagc gcgtggcctg gggtgccgac
     1921 gttcggctcg actttctcga tggaactttc ctggccggtt atcgacgtca tcccatccgt
     1981 atcgtggggg atggacg
//
LOCUS       DAOSOR010000790         1994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_56778_length_1994_cov_2.950490, whole genome shotgun sequence.
ACCESSION   DAOSOR010000790 DAOSOR010000000
VERSION     DAOSOR010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56778_length_1994_cov_2.950490"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="PK710_24930"
     CDS             <1..936
                     /locus_tag="PK710_24930"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HOT13050.1"
                     /translation="GIRVIVRGPWARRSRAIAYGALMTCWAIPMRSCTACGHDGANLL
                     LKRAKRRTNEHPFTPKQTMNKVYKNAREACADIPSGATMLLGGFGLCGIPENCILALR
                     DLGIKDIVMISNNCGVDDFGAGVLLQTRQIRKMVSSYVGENKEFERQFLEGELEVELV
                     PQGTLAERIRAGGAGIPAFYTPAGVGTAVSEGGLPMLYDKKGNVIKASEPKDIRVFDG
                     KMYVLERGITAQYALVKAWKGDRLGNLVYRHTAANFNPMMAMAGEITIAEVEELVEVG
                     ELDPDHIHTPGIYVHRIFQGETFEKRIERRTVSKS"
     gene            945..1607
                     /locus_tag="PK710_24935"
     CDS             945..1607
                     /locus_tag="PK710_24935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HOT13051.1"
                     /translation="MAALTRDQIAKRAAAELRDGYYVNLGIGMPTLVSNYIPKGMQVF
                     LQSENGLLGMGPYPTEDKIDADLINAGKETVTMVPGASIFSSADSFAQIRGGHINIAI
                     LGAMQVSETGDLANWMIPGKMVKGMGGAMDLVAAARRVVVIMDHCSKDGSPKVVRSCS
                     LPLTGRRVVHRIITDLAVMDVTPKGLLLIEVVEGMTAADIQQRTEPKLLIADNLKTIA
                     ID"
     gene            1604..>1994
                     /locus_tag="PK710_24940"
     CDS             1604..>1994
                     /locus_tag="PK710_24940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HOT13052.1"
                     /translation="MIPLPDLLSRVYGRAPRGIELGLDRVGRALDALDHPERNWASLH
                     IAGTNGKGSVAAIASLALQQAGYRVGLFTSPHLHRFAERIRIDQIPLDDDRLAQALAL
                     ALSFDLTFFETAFIAAIIAFHQAKTECA"
BASE COUNT          445 a          628 c          525 g          396 t
ORIGIN      
        1 ggaattcggg tcatcgttcg cggcccttgg gcgcgcagga gtcgtgccat cgcgtacggt
       61 gctctgatga cttgctgggc aatcccaatg cgatcgtgta cagcctgcgg gcacgatggc
      121 gcgaacctct tgctcaaacg cgccaagcgt cgtacgaacg agcatccttt caccccgaaa
      181 caaaccatga acaaagtcta taagaacgcg cgtgaagcct gcgcggacat tccttcgggc
      241 gccaccatgc tgcttggagg tttcggtctg tgtgggatcc ccgaaaactg catcctcgct
      301 ctacgagacc ttgggatcaa agacatcgtc atgatctcga acaactgcgg cgtcgacgat
      361 ttcggagcgg gtgtgctgct tcaaaccaga caaatccgca aaatggtgtc gtcgtatgtc
      421 ggcgaaaaca aggagttcga acgccaattt ctcgaaggag aactcgaagt cgaactggtt
      481 ccccagggga cgttggccga gcgcattcgc gcgggcggcg ctggcatccc cgccttctat
      541 actcccgccg gtgtgggaac cgccgtaagc gaaggtggac tacccatgct ctacgacaaa
      601 aagggcaacg tgatcaaagc ctcggaaccc aaggatatcc gcgtattcga tggcaaaatg
      661 tatgtgctcg aacgtggcat caccgcgcaa tacgccttgg tcaaagcctg gaagggcgac
      721 cggctcggca accttgtcta tcgtcacacg gcggccaact tcaatcccat gatggccatg
      781 gccggtgaaa tcaccatcgc cgaagtagaa gaactcgtgg aagtgggtga gttggatccc
      841 gaccatatcc acacccccgg catctacgtt caccgaatct tccaaggcga aactttcgaa
      901 aaacgcattg aacgacgtac cgttagcaag tcgtaggagc tttcatggca gcattgacac
      961 gtgatcagat tgccaagcgt gcggccgcag agctgcgcga cggctattac gtcaacctcg
     1021 gcatcggcat gcccacgctg gtatccaact acatccccaa agggatgcag gtctttttgc
     1081 agagcgaaaa cggattgctc ggcatgggac cgtatcccac ggaagacaag atcgacgcgg
     1141 acttgatcaa cgccggcaaa gagaccgtca ccatggtccc aggcgcatcg attttttcaa
     1201 gcgccgactc gtttgcccaa atccgcggcg gccacatcaa tatcgccatc cttggtgcca
     1261 tgcaggtctc cgaaacgggc gatctcgcca actggatgat ccctggaaaa atggtcaaag
     1321 gcatgggcgg tgccatggat ctcgtggccg cggcgcgacg ggtggtcgtc atcatggacc
     1381 attgttccaa agacggttca cccaaagtcg ttcgctcttg ctcgctcccg cttaccgggc
     1441 ggcgcgtcgt tcatcgtatc atcacggacc ttgccgtcat ggatgtcacc cccaaaggac
     1501 tgctgctcat cgaagtcgtg gaaggaatga cagcggctga catccaacaa cgcaccgaac
     1561 ccaagctcct catcgccgat aacctgaaaa ccatcgcgat cgattgatac ccctgcctga
     1621 cctgctctcg cgcgtttacg gtcgcgcccc ccgcggcatc gaactcggcc tcgatcgcgt
     1681 gggtcgcgcc cttgatgcgc tcgaccaccc ggaacgaaac tgggcctccc tacatatcgc
     1741 gggcaccaac ggcaaaggaa gcgtggccgc catcgcctcc cttgcccttc aacaagccgg
     1801 ttatcgggtc ggcctgttca cctcccctca tcttcatcgt ttcgccgaac gaattcgaat
     1861 cgatcaaatc cccctcgatg acgatcgact cgcccaggcc ctcgcccttg ccctctcttt
     1921 cgacctcacc tttttcgaaa cggctttcat cgccgccatc attgccttcc accaggcaaa
     1981 aaccgaatgc gccg
//
LOCUS       DAOSOR010000791         1980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_57325_length_1980_cov_3.353766, whole genome shotgun sequence.
ACCESSION   DAOSOR010000791 DAOSOR010000000
VERSION     DAOSOR010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57325_length_1980_cov_3.353766"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PK710_24945"
     CDS             <1..483
                     /locus_tag="PK710_24945"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13053.1"
                     /translation="ITLASIQASQDAAGVRRPYPGGDPSLVLIPPTQQYRKDYVFLTP
                     DKYAFDFLIVTAPASANVMIDGRPLDELECETAWANGGDAPTVAGVAEARVHRCPLSF
                     PFIDPLLPPEQGVFQGTQNDGVHRVQSNENVGLIVVGFDERVSYGYAAGTQLEAIAVP
                     "
     gene            complement(498..674)
                     /locus_tag="PK710_24950"
     CDS             complement(498..674)
                     /locus_tag="PK710_24950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13054.1"
                     /translation="MWSGWLERIATYVLEPSRDICTICGEIVSTARHCENCGVVVCTT
                     CSNEGVCPYCGEPL"
     gene            complement(791..>1980)
                     /locus_tag="PK710_24955"
     CDS             complement(791..>1980)
                     /locus_tag="PK710_24955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13055.1"
                     /translation="QGMTQEADDHLIAYGERLVFIGRVNEGLVALDDVARRRNTDANL
                     ARRVADLYLDRNGPGDAMLALTRMQACFLSTKRDIETLRVLARAFTIIGQRPKAFEVR
                     KLMVKIAYDNGDLEIAQALVDELRKDNPKDQELRTLEAMVYPDRAQPVPMPSVDAPQE
                     EAVPASVSLSEDSFEFIEESISELEGEMVAAASTYPPAHGPFINSHPNLPPVPPPAPI
                     PRPMSAMSPMQPPPPPVLPRPAVAPPAPRPVQHTAPTAQVWTIPSSIPPDPNDMELAE
                     AYDDFSPNDDLGELEETTLSRTAKPSPGSLPPPDFFADDDAADAFDDASFNEPSDAPQ
                     PDFIVDEGLEEAFEEADFFMGQGLYDDALTLLQDLLPRYPDHPLLIERIHFVSAAKYD
                     QDK"
BASE COUNT          447 a          544 c          567 g          422 t
ORIGIN      
        1 atcacactcg cctccattca agcaagccaa gatgccgcgg gagtgcgtcg accctatccc
       61 gggggggacc caagcttggt cctcatcccg cccacgcaac agtaccgaaa agattacgtt
      121 tttttgacac cggacaagta tgctttcgac tttctcatcg tgacggctcc tgcctctgcg
      181 aacgtcatga tcgatggtcg tcccctcgat gaacttgaat gcgaaaccgc atgggccaat
      241 ggtggggatg cccctacggt cgctggagtt gccgaagcgc gagtgcatcg atgccctttg
      301 agctttcctt tcatcgaccc gttgctccca cccgaacaag gcgtttttca aggaacgcaa
      361 aacgatgggg ttcatcgcgt gcaaagcaat gaaaacgtag ggttgatcgt tgtggggttc
      421 gatgaacggg ttagctacgg atatgcggct ggaacgcaac tcgaagcgat tgccgttcct
      481 tgaagaggtt tgaaaaacta cagcggttcc ccacaatacg gacagactcc ctcgttcgag
      541 catgtcgtgc aaacaacgac accgcagttt tcacagtggc gagccgtgga gacgatttca
      601 ccgcagatgg tgcaaatgtc acggctgggt tccagaacgt aggttgcaat tctctccaac
      661 caacccgacc acattgtcat catgttgctc caggggttaa acagacggct cagtcagaca
      721 acgtccgagg ccggacgttc cctacacccc cggtaacgct cgacgaaact cgatgtattc
      781 ccctgggtgt ctacttgtcc tgatcatatt ttgccgcgga aacgaagtga atccgctcga
      841 tgagcaaggg atgatccggg tagcggggaa gcaagtcctg cagcaatgtc aaggcatcat
      901 catacagccc ttgacccata aaaaaatccg cctcttcaaa ggcttcttcc agcccctcgt
      961 cgacgatgaa atcgggctga ggtgcatcag aaggctcgtt gaaacttgcg tcatcgaacg
     1021 catcggcagc gtcgtcgtcc gcaaaaaagt cgggaggagg tagcgagcca ggcgatggtt
     1081 tggcagtgcg agacagggtg gtttcttcga gttcccccaa atcgtcgttg ggtgagaaat
     1141 catcgtaggc ttcagccaat tccatgtcgt tcggatctgg agggatcgaa gatgggattg
     1201 tccaaacttg cgccgtcggt gccgtgtgtt gcacaggtcg cggggccggc ggagccacag
     1261 ccggacgcgg cagaaccggg ggcggcggtg gttgcatggg cgacatcgca ctcataggac
     1321 gtggaatcgg tgctggcggt gggacgggag gaaggtttgg atggctattg atgaaagggc
     1381 catgggcagg aggatacgtg ctggccgccg cgaccatctc cccttccaat tcgctgatgg
     1441 attcttcgat gaattcaaag gaatcttcgg acaaagacac actggcaggg acggcttcct
     1501 cttggggagc gtccaccgaa ggcatgggca ctggctgagc ccgatcggga tataccatcg
     1561 cctcgagcgt ccgcaactcc tggtccttcg gattgtcctt gcgtagctca tcgaccaacg
     1621 cctgggcaat ctccaaatcg ccgttatcat aggcgatctt caccatgagt ttacggactt
     1681 cgaacgcctt gggacgctgg ccgataatgg taaatgccct ggcaagcacc cgaagcgttt
     1741 cgatgtctcg tttggtagac aaaaaacacg cttgcattcg cgtcaatgcc agcatggcgt
     1801 cgccaggacc attgcgatcg agatatagat ccgccacgcg acgagccagg ttggcatcgg
     1861 tattgcgacg cctcgcgacg tcatcgagtg cgaccaaacc ttcattgaca cgaccgataa
     1921 acacgagcct ctcgccatag gcgatgagat gatcatcggc ctcttgggtc atgccctggg
//
LOCUS       DAOSOR010000792         1979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_57346_length_1979_cov_4.464137, whole genome shotgun sequence.
ACCESSION   DAOSOR010000792 DAOSOR010000000
VERSION     DAOSOR010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57346_length_1979_cov_4.464137"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1445
                     /locus_tag="PK710_24960"
     CDS             <1..1445
                     /locus_tag="PK710_24960"
                     /inference="COORDINATES: protein motif:HMM:NF018504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protein kinase PrkA"
                     /protein_id="HOT13056.1"
                     /translation="ISRRYRVGAVTLGPQMSVDAGERQVTADLSLAALPVALQATTLY
                     EAFGELVDAAGGLLEFSDLLKRPLDTYKYLQLSVEAGEVALQRQNLMLNCVMIGSANE
                     IHLAAFREHPEFPSFRGRMELIRTGYLLDYLAEKRIYDNQVAAQVQCHVAPHATLVAA
                     TFAVLTRMMKPDPDKYDQALRELASSLSALEKADLFACGLIPNRLNLDQRKTLRSNIP
                     SILAEGQSRSLYEGMIGASPREVQTVLLDAAQHPHYQCLSPLAVLEELDKLCERISQF
                     AWLQESPQEGGYHDHAAFRKLLLDRLHDTWEEEFRTASGLVDEKQYASLFQRYIENVS
                     AWSKGERIRNRITGEDTNPDTNLMEEMESLLGWDENREENRRGMLSRLAAWSLDHPKE
                     PLDHLLVFADQLKNVRETVYAKLRKPIAELCRDIVECIRHDGTMLSEERKKEVRDALD
                     ALAQQQGYCDQCAADAAAMLIRNRFRDIIV"
     gene            1448..>1979
                     /locus_tag="PK710_24965"
     CDS             1448..>1979
                     /locus_tag="PK710_24965"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOT13057.1"
                     /translation="MDQRRYRPLGPIALLALGINGIVGVGIFFIPSTVSSYIPGVWGA
                     SVYALTTLFCIPIALCFSRLGPIFDEDGGPYVFARAAFGPDAAFMVGWLTFSSAVLST
                     SAVIRGLSEAICTSYLTADAQRMVATATVLVLSAIVFGGLRITAWAWTTMTVAKLAPL
                     LALLVVWMAAPVAHHAS"
BASE COUNT          416 a          615 c          519 g          429 t
ORIGIN      
        1 tcatttcacg tcgctatcgc gtgggcgccg tgaccttggg accgcagatg tcggtcgatg
       61 ccggagaacg gcaagtaacc gcggatcttt ccctggccgc attgcccgtg gccttacaag
      121 caacgactct gtacgaggcc ttcggagaac tcgtcgatgc tgctggaggg ctactcgagt
      181 tcagcgacct gctcaagcgt cctctggata cgtacaaata tctccagctt tccgtggaag
      241 cgggagaggt ggcgctacaa cgtcaaaacc tcatgctcaa ttgcgtcatg atcggaagcg
      301 ccaacgaaat tcacctcgcc gctttccggg aacaccccga gttcccaagc ttccgtggtc
      361 gaatggaact cattcgcacc ggttatctcc tcgattacct cgcggagaag aggatctacg
      421 acaaccaggt ggcagcgcaa gtgcaatgcc atgtcgcgcc tcacgcgacg ctcgtcgccg
      481 caacgttcgc cgttctcacc cggatgatga agccggatcc ggataaatat gatcaagccc
      541 ttcgcgaact tgcgtcctct ctatccgccc tcgaaaaagc cgatctcttt gcttgcggcc
      601 tcattcccaa tcgattgaac ctcgaccagc gaaaaaccct tcgctccaat atcccatcga
      661 ttctggcgga agggcaatcg cgctcgttgt acgaaggtat gatcggcgca agcccgcggg
      721 aagtgcaaac cgtattgctc gatgcagctc agcatcccca ctaccaatgc ctatcacctc
      781 tcgccgtact cgaagaactc gacaaactct gtgaacgcat ctcgcaattc gcttggctcc
      841 aagaatcccc gcaagaaggc ggctatcatg accacgccgc gtttcgaaaa ctgctgctcg
      901 accgtttgca cgatacctgg gaggaagaat tccgaaccgc cagcggtttg gtggatgaaa
      961 aacaatacgc cagccttttc caacgctaca tcgaaaacgt atccgcctgg agcaagggcg
     1021 agcgtatccg caaccgcatc acaggcgagg atacgaatcc ggataccaac ctcatggaag
     1081 aaatggagtc ccttctggga tgggacgaaa atcgagaaga gaatcgtcgg gggatgctga
     1141 gtaggctggc tgcgtggtct ttggaccatc caaaagagcc tctcgatcat ctgttggtct
     1201 ttgccgacca gctaaaaaac gttcgggaaa ccgtttacgc caagcttcgc aaacccatcg
     1261 ccgaactgtg tcgcgacatc gtggaatgca ttcgtcacga tggcactatg ctttccgagg
     1321 agcgaaaaaa ggaagtccgc gacgccctcg acgctctcgc acagcaacaa ggctattgtg
     1381 atcaatgcgc cgccgatgcc gccgcgatgc tcattcgaaa tcgtttccga gatatcatcg
     1441 tgtagcgatg gatcaacgcc gttaccgccc cttgggtccg atcgcgctgc tcgcgctcgg
     1501 catcaacggg attgtgggcg ttggcatctt cttcatccct tccaccgtat cctcctatat
     1561 cccaggtgtc tggggagcca gcgtctatgc tctcaccacc ctgttttgca ttcccattgc
     1621 cttgtgtttc agccgtttgg gaccgatctt cgacgaagac ggaggaccgt acgtctttgc
     1681 ccgggcggcc ttcggtcctg acgccgcttt catggtcgga tggctgacct tcagctccgc
     1741 cgtgctgagc acctccgccg tgatccgcgg cctttccgag gccatctgca ccagctatct
     1801 cacggccgac gcccaacgca tggtcgcaac cgctaccgtt ctcgtcctgt ccgcgatcgt
     1861 ctttggcggg ttgcgtatca cggcatgggc ttggacaacg atgacggtgg caaagctcgc
     1921 tcccctgctg gccttgctcg tcgtgtggat ggcagcgccc gtcgcccatc atgcatcgt
//
LOCUS       DAOSOR010000793         1962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_58106_length_1962_cov_3.396959, whole genome shotgun sequence.
ACCESSION   DAOSOR010000793 DAOSOR010000000
VERSION     DAOSOR010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58106_length_1962_cov_3.396959"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PK710_24970"
     CDS             complement(<1..342)
                     /locus_tag="PK710_24970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOT13058.1"
                     /translation="MNANILLGLSPLLIVAVGALVLMVVEALSRFRDERLESPSASLA
                     VSSSVVLFASAIAAAGVWFYGPENLPEVSSLAPWLLLDRFSLFFFVILTLGGALSCLL
                     AGGYLPEHKLDR"
     gene            complement(339..>1962)
                     /locus_tag="PK710_24975"
     CDS             complement(339..>1962)
                     /locus_tag="PK710_24975"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOT13059.1"
                     /translation="PRQSPRVLRGLTLGILLVDLLASLWLLQVPMSTGWHFVYVHEWI
                     PAFGIRYHVAVDGISLWLVLLTTFITPIAAYVTFGSIKTRIKDLCFSFLFLQAAMIGA
                     FVALDLFLFYVFWELMLIPMYLMIGVWGSQDRIKAALKFFLYTMAGSVLMLAAMLYLV
                     YTYSQLTGEVSFSLLALNKVLLPQQAQLLCFWAFAIAFFIKVPMFPVHTWLPDAHVQA
                     PTGGSIILAAILLKMGTYAYLRFAMGLFPWAAGTQAANLAGVAVLGGIIYGALCSWKQ
                     DDFKRLVAYSSVAHLGYVMLGLFANTHTSTQGALLQMVNHGVSTGALFLLVGVIYDRR
                     HTREISEFGGLAKVMPVYAAVFLVVTMASVGVPGTNGFVGEFMVIMGTYTSPYLGKFH
                     WVDAAVAAFGVILAAVYMLSVVQKVFFGPLKNPKNAHLPDLNVREGIALVPLVIMIFV
                     IGLFPSVFLSRSSQSVSDFHTRNRLVWNESQAANPEPRLLTNDILAGSLSGSSRDELM
                     KRLEALDGQEPGRKPDDKGSKDETLAIQEESK"
BASE COUNT          513 a          589 c          546 g          314 t
ORIGIN      
        1 ccgatcgagc ttgtgttcgg gaagatagcc gcccgccagc aagcaggaca acgctccccc
       61 caacgtcaaa atcacgaaga aaaacaagct gaatcggtcg agcagcagcc atggagccag
      121 cgacgagact tccggcagat tctccgggcc gtagaaccac acgcccgcgg cggcgatggc
      181 cgaggcaaat aacacgaccg aagacgaaac ggccaagctg gcggaaggtg attcgagccg
      241 ctcgtcacga aaacgcgaaa gcgcctcgac caccatcagg acaagagcac ccaccgctac
      301 gatgagcaga ggcgacagcc cgagaaggat attcgcgttc acttgctttc ctcctggatc
      361 gccaaggtct cgtccttgga acccttgtcg tctggttttc gtccaggttc ttgcccatcc
      421 aaggcttcca gccgcttcat caattcatcg cgcgaagagc ccgacaggct gcccgccaaa
      481 atgtcgttgg tcagcaagcg cggctcggga ttcgccgcct gggactcatt ccacacaagc
      541 cgattgcgcg tgtggaaatc cgataccgac tggctcgatc gactcagaaa taccgaggga
      601 aatagcccga tcacgaaaat catgatcacg aggggaacga gcgcgatgcc ctcgcgcacg
      661 ttgagatccg gtagatgagc gtttttcgga tttttcaacg gcccgaaaaa cactttctgc
      721 accacactga gcatgtatac cgccgccaag atgacgccga aagccgccac cgccgcatcc
      781 acccaatgga atttgcccag ataaggcgag gtataggtgc ccatgatgac catgaactcg
      841 ccgacgaatc cattggttcc cggcacgccc acgctcgcca tggtcacgac gagaaacacc
      901 gccgcgtaca cgggcatcac cttcgccaac ccaccgaatt ccgagatttc tcgggtatga
      961 cgacggtcgt agatcacccc tacgagcaaa aagagcgcgc ccgtggacac accgtggttg
     1021 accatctgca acaaggcacc ctgggtgctc gtatgcgtgt tggcaaaaag gccgagcatg
     1081 acgtagccga ggtgcgcaac ggaagaatag gccaccaatc gcttgaaatc gtcttgtttc
     1141 catgaacata gggcgccgta gatgatgccg ccaagcaccg ctacccccgc caggttcgcg
     1201 gcctgggtgc ccgccgccca ggggaacaat cccatcgcaa aacgcaggta cgcataggtc
     1261 cccatcttca acagaatggc cgccagaatg atggagccgc ccgtgggcgc ctgcacgtga
     1321 gcatccggta accacgtatg caccgggaac atcggcactt tgatgaagaa agcgatcgcg
     1381 aaagcccaga aacaaagcag ttgcgcctgt tgcggcaaca gcaccttgtt caacgcaagc
     1441 aggctgaaac tcacctcacc ggtgagttgc gagtaggtgt atacgaggta caacatggcg
     1501 gcgagcatca gcaccgaccc ggccatcgtg tacaaaaaga acttgagagc cgccttgata
     1561 cggtcctgag aaccccagac gccgatcatc aagtacatgg ggatcagcat cagctcccag
     1621 aacacgtaga acaggaatag atccagcgcg acaaaggcgc cgatcatcgc cgcttgcaag
     1681 aatagaaacg aaaaacacaa gtccttgatg cgcgttttga tcgaaccgaa ggtcacgtac
     1741 gccgcaatgg gcgtgatgaa ggtggtcaga agcaccagcc aaagactgat tccatcgacc
     1801 gcgacgtggt aacggatgcc gaaagcgggg atccactcat gcacgtatac gaagtgccat
     1861 ccggtggaca tgggaacttg cagcagccac agggaggcga gcaagtcgac gagcagaatg
     1921 ccgagggtca ggccgcgcaa aacgcgtggg gattgccggg ga
//
LOCUS       DAOSOR010000794         1925 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_59666_length_1925_cov_4.589305, whole genome shotgun sequence.
ACCESSION   DAOSOR010000794 DAOSOR010000000
VERSION     DAOSOR010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59666_length_1925_cov_4.589305"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PK710_24980"
     CDS             complement(<1..617)
                     /locus_tag="PK710_24980"
                     /inference="COORDINATES: protein motif:HMM:NF014213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13060.1"
                     /translation="MRSPREYALQLLEYIDHCPTAFHAVERSVAWLSSAGFEPLREQD
                     KWQLTPGDRRFVIRNGSTIVAFVVGSAPPSEAGFLLVGAHTDSPALKLKPNASHQRFG
                     YQQLGVEKYGGLLLSTWFDRDLGLAGRVVLDRPGADLETQLIHIQRPILRIPTLAIHL
                     NRDVNSKGFLIDEQNHLPPIFGFAQSADEDALRKLLVHEFGRQGLA"
     gene            complement(675..1703)
                     /gene="dusB"
                     /locus_tag="PK710_24985"
     CDS             complement(675..1703)
                     /gene="dusB"
                     /locus_tag="PK710_24985"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOT13061.1"
                     /translation="MAFRIGPLNFEGAAWLAPLAAVSSAPFRQICLEHGCEQAVTEMV
                     SSEALVREVRKIARRMTRAQGERALIVQLFGSHPSVMAQAATLAVQTTHADAVDINMG
                     CPVRKVLGTGAGVALMRDPDRAAAIVESVCRAIAPVPVTVKMRAGWDDELNAVQVARS
                     VVQAGAKCITVHGRTREQYHKGETRWDIITAVKQAVAVPVIGNGGVRCASDAQAMLEQ
                     TGCDAVMIGRGALGNPWVFRNVARGVDQEPSLEQRFQVIRIHVARQVAYAGEFMGIRE
                     MRKHLGWYLRGLKGSAMVREKLHSMKTEQDVLSALSAYQEALTSGRATASQRDFEPDA
                     FPHSRYGV"
BASE COUNT          418 a          559 c          540 g          408 t
ORIGIN      
        1 gccaatcctt gtcgtccgaa ttcgtgaaca agcagcttgc gcaaagcatc ttcatccgcg
       61 ctttgtgcaa aaccaaaaat gggaggcaaa tggttctgct cgtcgatgag gaaacctttg
      121 gaattgacgt cacggttgag atgaatggca agcgtgggaa tacgcaggat gggtcgttga
      181 atgtgaatca attgggtttc gaggtcagcc cccggacggt ccaataccac acgcccagcg
      241 agtccaagat cgcgatcgaa ccaggtggac aataggagtc caccgtactt ttccacgcca
      301 agctgttggt atccgaaccg ttgatgagat gcgttgggtt tgagtttgag ggctggtgag
      361 tccgtatgcg ctcccaccaa aagaaatccc gcttcggacg gtggtgcgct gccgaccacg
      421 aacgccacga tggtactgcc gtttcggatg acgaagcggc gatctcccgg cgtgagttgc
      481 cacttgtctt gttcgcgcaa tggttcgaat cccgcggagg acagccacgc gaccgaacgc
      541 tcgaccgcat gaaacgccgt gggacaatga tcgatgtact ctagcaattg cagtgcgtat
      601 tctcggggcg atctcattcg agaagaataa acactgctat ccatcctgac caggacttca
      661 cacgtgttgg ggtatcagac accgtaacgt gagtgaggga aggcgtcggg ctcgaagtct
      721 ctttgggaag cggtggcgcg accgctcgtc agagcctctt gataggcaga aagcgcggac
      781 agcacgtctt gttcggtttt catcgaatga agtttttcgc gtaccatcgc gcttcctttc
      841 aagcctcgta gataccagcc aagatgcttt cgcatctccc gtatccccat gaattcaccc
      901 gcataggcca cttgcctcgc gacgtgtatc cgaatgactt gaaagcgttg ttcgagggaa
      961 ggttcctggt ccacaccacg agccacattt cgaaacaccc atgggttacc cagcgctccg
     1021 cgaccgatca tcaccgcatc acagcctgtt tgctcgagca tggcctgcgc atccgaggcg
     1081 caacgcaccc cgccattgcc gatcacggga acagcaaccg cttgtttgac ggcggtgatg
     1141 atgtcccagc gtgtttcgcc tttgtggtat tgctcgcgcg ttctgccatg caccgtgatg
     1201 cattttgctc cggcttgcac cacgctccgt gcgacctgga ccgcgttgag ttcgtcgtcc
     1261 cagccggcac gcatcttgac agtcaccgga accggggcaa tcgcacgaca cacgctttcg
     1321 acaatcgcag cggcgcgatc gggatcgcgc atcaacgcaa ccccagctcc cgttcccaac
     1381 accttacgaa cgggacaccc catattgata tccaccgcat ccgcgtgggt cgtctgcact
     1441 gccagggtcg cggcctgggc catcacggaa ggatggctgc caaaaagctg gacaatgagt
     1501 gcccgctcgc cttgcgcgcg tgtcatacgt cgcgcgatct tacgaacctc acgcaccaat
     1561 gcctcactgg acaccatttc ggtgaccgct tgctcgcagc catgctcgag gcatatctga
     1621 cggaacggag cactgcttac cgcggcaaga ggcgcaagcc aggccgctcc ttcgaaattc
     1681 aggggaccga tccgaaatgc catgacggct tgccatttgc ttcacgaagc tggggttggc
     1741 aagactcgag gcacattcat cgttcgtgca tccgaggctc gtcgctgtag ctgcctgcat
     1801 cgggaggcct cctaaagcaa cgcggatcaa ggcttgaaca aaaggaaagt agacctattt
     1861 gtgatgtgat ttcaatatgt tatgacaggt gcgccggtcg tggaaggcgg cgggcgtaaa
     1921 gcgag
//
LOCUS       DAOSOR010000795         1904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_60585_length_1904_cov_4.135208, whole genome shotgun sequence.
ACCESSION   DAOSOR010000795 DAOSOR010000000
VERSION     DAOSOR010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60585_length_1904_cov_4.135208"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="PK710_24990"
     CDS             complement(<1..942)
                     /locus_tag="PK710_24990"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOT13062.1"
                     /translation="MSAVIGIDLGTTNTVVAAVRDGRAMALPTETGERLFPSVVSFHP
                     NGSVLVGREAKRRRLQDAANTVYSTKRLIGRSYDSEEVRKARSRSAFVIQEGPGRGVL
                     VEARGETYTLSEISAFVLKEAKRIAESTGEVVDRAVITVPANFNDLQRAATKVAGHIA
                     GLEVQRILNEPTAAALAYGYGKGNQERIAVFDFGGGTFDVTLLDLAGSVFEVLATAGD
                     TFLGGDDIDLAIAERMAERYLKTHRYDARADRQVFERLLASAEEIKIRLSASDVATVQ
                     LQEIAFGVGGKSLDMPFSMTRLELEQLAAPLVDRALGV"
     gene            complement(970..1533)
                     /locus_tag="PK710_24995"
     CDS             complement(970..1533)
                     /locus_tag="PK710_24995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13063.1"
                     /translation="MMEDYRVPYEALLDEIRNEFNDFKIVHKRTDRLSHRIDRLLCWV
                     TLGGQREYLTRYHTVLGNTLYVPDGWDSTPYLDRIITLRHERVHLRQRRRYGGIGMAF
                     LYLIPFFPLGLAYGRARIEWEAYAETVLATAELKGVETARSASMREYVVRQFTSGAYG
                     WMWPFRRQVDAWYDEVLAKLDRQVSRR"
     gene            complement(1530..>1904)
                     /locus_tag="PK710_25000"
     CDS             complement(1530..>1904)
                     /locus_tag="PK710_25000"
                     /inference="COORDINATES: protein motif:HMM:NF014908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOT13064.1"
                     /translation="PQPQKEAQATYAPIIRKEDGKIDWSHSARAIHAHIRGMTPWPGA
                     FTELGGQWLKVHRARHHDGQAGPSGTVVIADSKHVLVACEAGLLELLQVQLPGKKAMP
                     ATALVAGRKLRAGDKLGAVGAG"
BASE COUNT          415 a          686 c          429 g          374 t
ORIGIN      
        1 cacccccagc gctcgatcca caagcggcgc cgcgagttgt tcgagttcca atcgcgtcat
       61 cgaaaacggc atgtccagcg atttgccacc gaccccaaac gcgatttcct gaagctgaac
      121 cgtggccaca tccgaagcag atagacggat tttgatttct tccgccgacg cgagaagccg
      181 ctcgaagacc tgacggtccg cccgagcatc gtagcgatgg gtttttagat aacgttccgc
      241 cattcgctca gcgatggcca gatcgatgtc gtctccgccc aaaaacgtat cgccggcggt
      301 cgccaacacc tcgaatacac tgcccgccag atcgagcagc gtgacatcga acgtaccacc
      361 cccaaagtcg aataccgcaa tacgctcctg gttgcctttc ccataaccat acgcaagcgc
      421 cgccgccgtc ggctcgttga gtatccgctg aacctcgagc cccgcgatat gaccagccac
      481 tttcgttgcg gcacgctgca aatcattgaa gttggcagga accgtgatga ccgccctatc
      541 caccacctcg cccgtcgatt cggcaattcg cttcgcctct ttcagaacga acgcgctgat
      601 ctccgataat gtatacgttt ccccccgcgc ctccacgagc actccacgcc ccggcccttc
      661 ctgaatcaca aacgccgagc gcgaccgtgc cttgcgcacc tcctccgaat cataagaccg
      721 tccaatcaac cgcttggtcg aatataccgt attcgccgca tcctgaagcc gacgacgctt
      781 cgcttcccga cccaccagga ccgaaccgtt cggatgaaac gatacgaccg acggaaacaa
      841 acgctccccc gtctccgtcg gcaacgccat cgctcgcccg tcgcgaaccg cagccactac
      901 ggtattcgtt gttcctaagt cgatcccaat tactgcactc attcttcccc catcgcggga
      961 ttgcttctct taccgccgag acacttgcct gtccagtttc gccaacacct catcatacca
     1021 agcgtccacc tgtcgccgaa acggccacat ccatccatac gccccactcg taaactgccg
     1081 caccacgtac tcacgcatcg acgccgaacg cgccgtctcc acgcctttca actccgccgt
     1141 cgccaacaca gtctccgcat acgcctccca ctcgattcgc gccctgccat acgcaagacc
     1201 caaggggaaa aacggaatga gataaagaaa cgccatccct atccccccgt aacgacgtcg
     1261 ctgacgcaaa tgcacccgct cgtgccgaag cgtgatgatc cgatccaaat acggcgtact
     1321 atcccaaccg tccggtacgt aaagcgtgtt gcccaatacc gtgtgatacc tcgtcaaata
     1381 ctcgcgctgc ccccccaacg tcacccaaca caatagccgg tcgatccgat gagaaagacg
     1441 atccgttcgc ttgtggacga tcttgaaatc attgaattca ttacgaattt cgtcaagcag
     1501 cgcttcgtag gggacgcggt agtcctccat catcctgctc ccacggcccc gagcttgtcg
     1561 cccgcccgaa gcttgcgtcc ggccaccagg gccgtcgctg gcatcgcctt ttttcctggc
     1621 aattggacct gcaatagctc gagcaaaccg gcttcgcatg ccaccagaac atgcttcgaa
     1681 tcggctatca ccacggtccc ggacggacca gcctgtccat cgtgatgccg cgcccgatgc
     1741 accttcaacc attgcccgcc caattccgta aaagcgcccg gccaaggcgt catccctcga
     1801 atatgcgcat gaatcgctcg cgcggaatgc gaccaatcga tcttgccgtc ttccttgcga
     1861 atgattggcg catacgtggc ctgcgcctcc ttttggggtt gagg
//
LOCUS       DAOSOR010000796         1903 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_60631_length_1903_cov_4.440476, whole genome shotgun sequence.
ACCESSION   DAOSOR010000796 DAOSOR010000000
VERSION     DAOSOR010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60631_length_1903_cov_4.440476"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="PK710_25005"
     CDS             <1..947
                     /locus_tag="PK710_25005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOT13065.1"
                     /translation="IGEQSTLEQMGGAHMHCSVSGCGDVLVYSEKEAIAWAQRYLSYL
                     PSRAGAAIPMAAPRPPALPWQIIEQVVPKDEGLPFDMMEVVKALVDEGSWLEIKPLYA
                     KEVLTGLCRIEGRSVGIVANQPNHLGGVLFVESANKVARFIQLCDAFGIPLVFLTDVP
                     GFMIGVKVEQQGIIRAGAKMIAAVSSATVPKISVIVRKAYGAGLYAMCGPAFEPDTCL
                     ALPSAAIGVMGPAAAVNAVYYNKIQALAEGEERQSFIEQKRQEYEEDIDLYKLADDLV
                     VDAVVPPSDLRRQLSHRLALLVDKQEIRLPRKHLVSPM"
     gene            complement(1224..>1903)
                     /locus_tag="PK710_25010"
     CDS             complement(1224..>1903)
                     /locus_tag="PK710_25010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13066.1"
                     /translation="WATGKQGARIFMALAARAFLLNKEHRCSDPDATPCSGTTSACHG
                     YYRESDVAHHTANTFHLLHRYLSDNDPDTRFVQLHGFAGNENDYVEASDGSRNKNNPS
                     SVSIAFINALKKLVPTPNAVFSCQDPKDPPPLGLCATTNVQARATHDPFIDACTEGTK
                     KSSGRFLHIEQAKALRQDSPSKGWSWTQVRDALHETWPDCRQPGCALGPPLPNAEGCT
                     CGMPCDP"
BASE COUNT          388 a          495 c          590 g          430 t
ORIGIN      
        1 tcatcggcga gcagagcacg ctcgagcaga tggggggcgc tcacatgcat tgcagcgtgt
       61 cgggatgcgg cgacgtgctg gtgtattcgg aaaaagaagc cattgcgtgg gctcagcgct
      121 acctatcgta cttgccgtcc cgagcgggag ccgccatccc catggcggcc ccaagaccac
      181 ccgccttacc ttggcaaatc atcgaacagg tcgttcccaa ggatgaggga ttgcctttcg
      241 acatgatgga ggtggtgaaa gcccttgtgg atgaaggctc ctggctggag atcaaaccgt
      301 tgtacgccaa ggaggtattg acaggcttgt gcagaatcga aggccgttcg gttggcatcg
      361 tggctaacca acccaaccac cttggcggcg tgctcttcgt ggaaagcgcc aacaaagtgg
      421 caagattcat tcaactttgc gatgcattcg gcattccttt ggtgtttctg accgacgttc
      481 cggggttcat gattggcgtc aaggtcgagc agcaagggat tatccgagcg ggcgcgaaaa
      541 tgatcgccgc ggtgtcatcg gccacggtgc ccaaaatctc ggtcatcgtc cgaaaagctt
      601 atggtgccgg tctttatgcc atgtgcggcc ccgccttcga acccgacacc tgccttgcgc
      661 taccctcggc agccatcggc gtcatggggc ccgccgccgc cgtcaacgcc gtgtattaca
      721 acaaaatcca ggctctcgcg gaaggcgagg aaagacaaag cttcatcgaa caaaaaaggc
      781 aagagtacga agaagacatc gatctttata aattggccga tgatctggtg gtcgatgccg
      841 tggttccccc ttccgacctt cgacggcaac tcagtcaccg actggcgctt ctcgtcgaca
      901 agcaagagat ccgacttcca cgaaagcacc tcgtttcccc catgtagcga gtcttccgac
      961 acttcctcgc catcgttggg ctgtgtccat ttaacatatt gaatttattg tctttttttg
     1021 gcttacccag cggttccgat cgtcggaaac catgacgcat acgcggacga tgccatgagt
     1081 ccatgcttgc gttgtggatg ccgaaggatt ggagagcgtc ggccgagaac catgttcgga
     1141 cggcagtcat cgatggactc ctgggattgg gtgtcggcga gttcagggag gaagtgaaac
     1201 gtcaggtctg gccacgggag cggtcaaggg tcgcaaggca ttccgcacgt gcagccttcg
     1261 gcgttgggta acggggggcc aagagcacag cctggctgcc gacaatcggg ccatgtctcg
     1321 tgcaacgcgt cgcgaacctg ggtccaggac catcccttcg acgggctatc ctgtcgcaaa
     1381 gctttggctt gttcgatatg aaggaaacgg ccggatgatt ttttcgttcc ttccgtgcag
     1441 gcatcgatga aagggtcgtg agttgctctt gcctgcacgt tcgtggttgc gcacagtccc
     1501 agggggggcg ggtctttcgg gtcttggcaa gagaacacgg cattgggtgt tggcaccagc
     1561 tttttcaggg cgttgatgaa agcgatggaa accgacgagg ggttgttttt gttccggctt
     1621 ccatcgctgg cttcgacgta gtcgttttcg ttgccggcaa agccgtgcaa ttgcacgaag
     1681 cgggtgtctg ggtcgttatc ggatagatag cgatgaagaa ggtgaaaggt gttggcggta
     1741 tggtgagcga catccgattc tcggtagtat ccgtgacacg cggaggtggt tccgctgcag
     1801 ggggtggcat ctgggtctga gcagcggtgt tctttgttga gaaggaaagc gcgagcggcg
     1861 agagccatga aaattctggc gccttgtttt cccgtggccc aat
//
LOCUS       DAOSOR010000797         1903 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_60640_length_1903_cov_3.751623, whole genome shotgun sequence.
ACCESSION   DAOSOR010000797 DAOSOR010000000
VERSION     DAOSOR010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60640_length_1903_cov_3.751623"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PK710_25015"
     CDS             <1..468
                     /locus_tag="PK710_25015"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241772.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOT13067.1"
                     /translation="MVRAYTYIRELGAEGLKQATDMAVLNANYLAALLKDTFPLAQEK
                     TSMHEAVFSDRVLEAETGIKTLDIAKRLIDYGFHPPTVYFPLVVKGAMMIEPTETESK
                     ETIEAFVSAMKSIEREARESPELVREAPHLTRLDRLDEARAARKPRLRWSQEP"
     gene            complement(475..915)
                     /locus_tag="PK710_25020"
     CDS             complement(475..915)
                     /locus_tag="PK710_25020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT13068.1"
                     /translation="MLSRPMNKADYDEIVRVIDWWWGGPTASLAHPVFFYELGKIARI
                     MEVDGKMIGFLLGFLTAEPPPIGYIHMVGIHPDYRRRHVGRKLYESFEDSCRSAGCTL
                     MKAVTAPGDERSVQFHEALGWVWETVPDYAGPGRARVVFTKRIG"
     gene            985..1686
                     /gene="rpe"
                     /locus_tag="PK710_25025"
     CDS             985..1686
                     /gene="rpe"
                     /locus_tag="PK710_25025"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOT13069.1"
                     /translation="MPKPVGSPSGPIVAPSILSADFGRLHDQVQAAEQAGAGWIHVDV
                     MDGHFVPNITIGPLVVRAVRRATQLPVDVHLMVSEPDSLVEAFVDAGADVVTVHSEAC
                     VHLQRTLRRIRDLGASVGVALNPHTPETVLSYVGRDIDLVLVMTVNPGFGGQAFLPSV
                     VPKIRALRSWVDEAGLPIVIEVDGGIGPGTAALVARAGARVLVAGSAVFGQPDLRKAI
                     EAIEREARVEAEVTS"
     gene            1683..>1903
                     /locus_tag="PK710_25030"
     CDS             1683..>1903
                     /locus_tag="PK710_25030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13070.1"
                     /translation="MRIVIGLGVVMALALGSCAPASPSKPAGSGGQEARTGVAASGVE
                     QVDAATQGMIDRMLERVSGMRGLSRVGVVR"
BASE COUNT          372 a          440 c          665 g          426 t
ORIGIN      
        1 atggttcggg cgtacacgta tattcgtgag cttggcgccg aggggctcaa gcaggccacg
       61 gacatggcag tgctcaatgc gaattatctt gcggctttgt tgaaggatac atttccgctt
      121 gcgcaagaga aaaccagcat gcacgaggcg gtgttttcgg atcgggtatt ggaagcggaa
      181 acagggatca agacgctcga tattgcgaag cgtctgatcg actacgggtt ccatccgccg
      241 accgtatact tccctttggt cgtgaaggga gcgatgatga tcgagcccac ggaaacggag
      301 tcgaaggaga cgattgaagc gttcgtgagt gcgatgaaat cgattgagcg ggaagcaaga
      361 gagtctccgg aattggtgcg agaggcgccg catctgacca ggttggatcg tcttgatgaa
      421 gcgcgtgcgg cgcgaaaacc gcggcttcgt tggtcacaag agccctgagg gagtttatcc
      481 aatccgcttc gtgaacacca cgcgggcccg accaggaccg gcgtagtcgg gcactgtctc
      541 ccaaacccat ccaagtgctt catgaaattg gacggaacgc tcatcgccag gagcggtcac
      601 ggctttcatg agcgtacagc cggcgcttcg acaggagtct tcgaacgact cgtacagttt
      661 tcgtccaaca tgccgtcgac gatagtcggg atgaattcca accatgtgga tgtagccgat
      721 gggggggggt tcagcagtaa gaaagccgag caaaaacccg atcattttcc catccacttc
      781 catgattcga gcaatttttc ccagctcgta aaagaatacg ggatgggcga gcgatgcggt
      841 tggaccaccc caccaccaat cgatgacacg gacgatttca tcgtagtccg ctttgttcat
      901 gggtctggag agcatcgaga cgcatggtat cagaattcgg ttccgatgtt gttctgccgt
      961 ggttcatgct aacgcaatct tgtgatgccg aagccagtag gaagtccttc gggacctatc
     1021 gttgcaccgt cgattctatc tgctgatttt ggccgcttgc acgaccaagt gcaggctgcc
     1081 gagcaagccg gagcgggatg gattcacgtg gacgtgatgg acgggcactt tgtgccgaac
     1141 attacgattg ggccgcttgt cgttcgtgcg gttcggcgag ccacccaact acccgtggac
     1201 gtgcatttga tggtgtcgga gcccgattcg ttggtggaag cgtttgtgga tgcgggagcg
     1261 gacgtggtga cggttcatag tgaggcttgt gtgcatttgc agcggacgtt gcgcagaatt
     1321 cgggatcttg gggcatcggt gggggtggcg ctcaatccgc atacgccgga aacggtgctt
     1381 tcgtacgtag gacgggatat cgatttggtg ttggtgatga cggtcaatcc tggtttcgga
     1441 ggacaagcgt ttcttccgtc ggtggtgccg aaaatccggg cgcttcgttc gtgggtggac
     1501 gaggcggggt tgccgatcgt catcgaggtg gatggtggca tcgggccggg cacggcggcg
     1561 ctagtggcta gggcgggcgc tcgggtactg gtggcgggtt ctgcggtttt tgggcaaccg
     1621 gaccttcgca aagccataga ggcgatagaa agggaggcga gggtggaggc cgaggtgacg
     1681 tcttgagaat tgtgattggg cttggggtgg tgatggctct ggctttggga tcgtgcgcgc
     1741 cagcttctcc gtcgaagcct gcgggttcgg gtgggcagga ggctcgtacg ggtgtggctg
     1801 catcgggcgt ggagcaggtg gatgctgcga cgcaggggat gatcgatcgc atgttggagc
     1861 gggtgagtgg gatgcggggg ctttcgaggg tgggggtggt gcg
//
LOCUS       DAOSOR010000798         1888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_61336_length_1888_cov_4.280415, whole genome shotgun sequence.
ACCESSION   DAOSOR010000798 DAOSOR010000000
VERSION     DAOSOR010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61336_length_1888_cov_4.280415"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PK710_25035"
     CDS             <1..335
                     /locus_tag="PK710_25035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13071.1"
                     /translation="RLLGIQQGVEQGVQQGLEQGVKQGVKQGLEQGVKQGVKQGVKQG
                     VKKGLDKGLAPLVRLYERRLGRALSSRERTALQERLLKAGPERVGDVVLDLSAQQLAD
                     WLADPNAT"
     gene            363..1214
                     /locus_tag="PK710_25040"
     CDS             363..1214
                     /locus_tag="PK710_25040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13072.1"
                     /translation="MANTKAENTISTNYSRIFVRYSTAGDLRAQWEEARTKGATLLPV
                     RSPPRHLTALERLTLTVQAPDIQADIEVELLQILEGLGIVVRVLDLGILDPPSLPMLG
                     TGSPTLPTVRFENSTETPSSHSCPEKRAPERTHDSAVGSSALSWSVDKLTAEWDNLGT
                     AERIRVARYGNRGARGVVLRSNDRTIFSHLLTNPHISTEEIASLAGNAALDPAALRRI
                     AMSTDWLRSAEVVRNLVCNPKLPMPEVERLIRLLSRDELVRLAKSGRARASVKQAVAK
                     RLKASER"
     gene            1433..>1888
                     /locus_tag="PK710_25045"
     CDS             1433..>1888
                     /locus_tag="PK710_25045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HOT13073.1"
                     /translation="MALLVVGPWLMLAVMVIFTPLPMELRADHRFDSSVRVFDRHGKL
                     LAEVRAEDGARARWVSLERVGSHLPNALIAAEDTRFRMHPGIDPFAMVRAAFHNLLHR
                     RIVSGASTLTQQLGRNLVPRPRTYWGKIREMTVALRIEASLSKDQILESY"
BASE COUNT          407 a          515 c          588 g          378 t
ORIGIN      
        1 gccggctgct ggggattcag caaggggtcg agcagggcgt ccagcagggt ctggaacaag
       61 gcgtcaagca gggcgtcaag cagggcctgg aacaaggcgt caagcaaggc gtcaagcaag
      121 gcgtcaagca gggcgtaaaa aaaggcctgg acaaagggct cgctccgctc gttcgtcttt
      181 atgaacgacg tcttgggcgc gcgttgtcga gccgcgaacg aacggcattg caggagcgtt
      241 tgctcaaagc agggccagaa cgcgtgggcg atgtcgtgtt ggatttgtcc gcccagcagc
      301 ttgcggactg gcttgcggat ccaaatgcca cctaaaaggc atggatataa cctgtcgaat
      361 cgatggcaaa caccaaagcg gagaacacca tttctacgaa ttattcacgc atcttcgtgc
      421 gatactcgac ggcgggggac ttgcgagcac aatgggaaga ggcacgaacc aagggagcga
      481 cccttctccc ggtcaggtcg ccgccccgtc atctcaccgc gctcgaacgt ctgacgttga
      541 ccgtccaagc ccctgatatt caagcggata tcgaagtgga gctgctgcag attctcgagg
      601 gactcggcat cgtggtgcgc gtgctcgacc tcggcatatt ggatccacct tcgctgccga
      661 tgctcggaac gggttcaccc acgcttccta ccgttcgctt cgagaattcg accgaaacgc
      721 cgtcgagcca ttcctgcccg gagaagcgtg ctcctgaacg aacgcacgat tccgccgttg
      781 gttcgtctgc cctttcgtgg tccgtcgaca agctgaccgc agaatgggac aatcttggca
      841 cggcggagcg gattcgggtg gcgcgatatg gcaatcgagg agcgcggggg gtggtattac
      901 ggagcaacga tcgaacgata ttttctcacc tattgacgaa tccccatatt tcgacggagg
      961 aaatcgcctc gctggcgggt aatgcggcgc tcgatccggc agccctgcga cggattgcga
     1021 tgagtaccga ttggttgcgt tccgcagagg tggttcgtaa cttggtgtgc aatcccaaac
     1081 tgccgatgcc cgaggtggaa cgtctgattc ggctgctgtc gcgagacgaa ttggttcggc
     1141 tggccaagtc cggacgcgcc agggcttccg tcaaacaagc cgtagccaaa agactcaaag
     1201 ccagcgaacg ttgaaaacca gtgagaagag gatgaaaaca cgaggcacgt ccatagccag
     1261 aaaccgacgt gatatcgcca tcttcggtgg atatcgccag agacgtggga atagccggca
     1321 agcaccgcgg cgttggtgaa ccatgtcgca agcatcaggt ttttggcaac ggcaacgccg
     1381 ccgggtacgc atagcttggg ctggatttcg acggccatgg gcgatgcgga ccatggcgct
     1441 tttggtggtg ggtccttggc tgatgctggc cgtgatggtc atttttacgc cgcttcccat
     1501 ggagcttcgc gcggaccatc gattcgactc ttccgtgcgg gttttcgatc gccatgggaa
     1561 actgctggca gaggttcgcg cggaagacgg ggctcgagcc cgatgggttt ccctcgagcg
     1621 tgttggctcc caccttccga atgccctgat agcggctgag gatacgcgat ttcggatgca
     1681 tccagggatc gatcccttcg cgatggtacg cgcagctttt cataatttgt tgcatcgccg
     1741 gatcgtatcc ggagcatcga cactcacgca gcagctagga agaaacctgg tgcctcgacc
     1801 gaggacgtat tggggaaaaa tacgagagat gacggtggct cttcggatcg aggcatcgct
     1861 atccaaagac cagatcctcg aatcgtac
//
LOCUS       DAOSOR010000799         1884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_61532_length_1884_cov_3.559322, whole genome shotgun sequence.
ACCESSION   DAOSOR010000799 DAOSOR010000000
VERSION     DAOSOR010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61532_length_1884_cov_3.559322"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..>1884
                     /locus_tag="PK710_25050"
     CDS             266..>1884
                     /locus_tag="PK710_25050"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT13074.1"
                     /translation="MAEPRQRAEPVAKGGDVEPASRVGTVIRGKWRVDSLVGSGGMAS
                     VYAATHRNGQTAALKVLHTSLSNETSIRDRFLREGYVANSVNHPGVVAVLDDDETDDG
                     APFLIMELLLGQTLSKRWKKLGRRMPIVEAFRIALPVLDCLSACHAASVIHRDLKPPN
                     IFLTDDGRVKILDFGVAQLRDATTEKTRTGTALGTPYYMSPEQAMGLVDQLDGRADLF
                     SVGAILHALITGQRIHNARTENEALILAATTPVPTVARIDPNLPIEVIKLIDKSLAWD
                     RRNRHADATAMREDVERTLALVQGQPSTAPVRMPSAREVAAIPEAPAARGVARPSSPS
                     RPAQPPQTVPTRSVPSHRPSIPPSVVEEETAEEDDPRVQEARDLFKHIDRLLPTGRQL
                     GLEHPATERAMRTSYEAFVLALNRQPQALRWKVRPYSFLVLNQTVWEPGAPFDTIPYN
                     MFAAGVRQFVMLPGLAFEEFRTVVALMMLDPGQDLPPEDDLVSAFWDRGLSHVAFETT
                     DPFTDGDAGQREAFVDEADELEDLAARASEA"
BASE COUNT          376 a          469 c          613 g          426 t
ORIGIN      
        1 cttgtgcgag gattggggag catatcgaaa ccaatgtgcg gcataaccgg acttcatagg
       61 agcaagggcc aaggaacgca acgatggtcg ggccgcatct cgaatcgtgg agaatactga
      121 attgtgcaga gaattcgatt gtcatttcgg ggtgggtctg ggaacaattt gacgatgact
      181 tcaatgttga cgtggggctt gacctagcgc tgggaaagat gttcaaggta cactcgacat
      241 aacattgact ggatatagtg aaccggtggc tgaaccacga cagagagcgg aacccgttgc
      301 caaaggagga gatgttgaac cagcgtctcg cgtaggcacg gtcattcgtg ggaaatggcg
      361 tgtcgactcg cttgtgggtt cgggcggaat ggcgtcggtg tatgcggcga cgcaccgaaa
      421 tgggcagacg gcggctctca aagtcctaca tacgtcgtta tccaacgaga cgagcattcg
      481 cgatcggttt cttcgtgagg ggtatgtcgc caattcggtg aaccatcctg gagtggtagc
      541 ggtactcgac gacgatgaga cggacgatgg tgctccgttt ttgatcatgg agctattgct
      601 tggtcagacg ctttccaagc gttggaagaa gcttggtcgt cgtatgccga ttgtcgaagc
      661 atttcgcatt gccctgcccg tattggattg tttgtccgcg tgtcatgcgg ccagcgtgat
      721 tcatcgtgat ctcaagccac cgaacatttt tttgacggac gatggtcggg tcaagatcct
      781 ggactttggt gttgcgcagc ttcgggatgc gacgacggag aagacgcgaa cgggcacggc
      841 tcttggcacg ccgtattata tgtctcccga acaagcgatg gggcttgtgg atcaactcga
      901 tggtcgggcg gatttgtttt ccgtgggagc gattctgcac gcgttgatca cggggcagcg
      961 gattcacaat gctcgaacgg aaaacgaagc tctcatcctt gcggcaacga cgccggttcc
     1021 tacggttgcg cgcattgacc ccaatttacc catcgaagtc atcaagctca ttgacaaatc
     1081 cctcgcctgg gatcgtcgca atcgtcatgc ggatgcgacg gcgatgcgcg aggatgtgga
     1141 gcggacgttg gcgttggtgc aagggcagcc ttcgaccgcg ccagtccgaa tgccatcggc
     1201 ccgcgaggta gcggccattc cggaagcgcc tgcggcgaga ggggtggctc ggccttcctc
     1261 gccttctcga ccggcccaac caccgcaaac cgttcccacg cgctccgtgc cttcgcatcg
     1321 gccttcgatt cccccatcgg tggtggagga ggagacggcg gaggaagacg acccccgtgt
     1381 gcaggaagca cgcgatttgt tcaaacatat cgataggtta ctgccaactg ggcggcaact
     1441 cgggctggag catcctgcga ccgaacgcgc gatgcgcacg agttatgaag cgtttgttct
     1501 tgcgctgaac cgtcaacctc aggcgttgcg gtggaaggtt cgtccttatt cgtttctcgt
     1561 tttgaaccaa acggtgtggg agcctggtgc gccgttcgat accattccgt acaacatgtt
     1621 tgcggcgggg gtgcgtcagt ttgtgatgct tccggggctc gccttcgagg aattccgtac
     1681 ggtcgtggcg ttgatgatgc tcgatccggg gcaagatctg ccgccagagg atgatttggt
     1741 atcggcgttt tgggatcgtg gtctttcgca cgttgcgttc gagacgacgg atccgtttac
     1801 ggacggcgat gcggggcaac gagaggcgtt tgtggacgag gcggatgagt tggaggactt
     1861 ggcggcacga gcatcggagg cgag
//
LOCUS       DAOSOR010000800         1840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_63624_length_1840_cov_3.324930, whole genome shotgun sequence.
ACCESSION   DAOSOR010000800 DAOSOR010000000
VERSION     DAOSOR010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63624_length_1840_cov_3.324930"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="PK710_25055"
     CDS             <1..710
                     /locus_tag="PK710_25055"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOT13075.1"
                     /translation="TNQDDDSFASLFENATTVQRQRHWSIGDRVEGIVVKIGSNAVFL
                     DLDGKREAFIDRASLADVTGQEATLSVGDTIVARVAEVGRHVGGVRLEPLSIRAAGQQ
                     EQSERVELVDAAGPRLSTGAHVKGQIVRVESYGVFLQIEGTTGREGRGLIPASETGSP
                     KGTDLRKKFPLGTNLEAKVLSIADDGKIRLSIRALQADEERASFESFSDHSNDHQGGF
                     GTMAAAFARSKNKSNKR"
     gene            786..1694
                     /locus_tag="PK710_25060"
     CDS             786..1694
                     /locus_tag="PK710_25060"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT13076.1"
                     /translation="MPLAPLPNPENIKLRLRGLLVAMPAVAFLGSSLMAANAVQMASL
                     PVLPISRSAFRKTNRWVANTWWGWCVTAGEVIHDISLVVSGDDVPMRENAIVIVNHQD
                     MADITFVMAFAKSKDRLGDLKWFVKKAIKIVPGVGWGMVFLDCIFVDRQWTSDRASVQ
                     AAFRRFSRDSIPIWLVSFVEGTRLTPEKLVQSQRYARQLGIEPLDHVLLPRTKGFVAS
                     VQGLRDHVDAVYDLTLGYEHGVPSLWQYIQGFSPRAHLHVRRFPMADLPHDPEALAAW
                     LHARFREKDELLSRYYQSGSFQETGS"
BASE COUNT          400 a          532 c          492 g          416 t
ORIGIN      
        1 ccacgaatca agatgacgac tcctttgctt ccctcttcga aaatgcaacg actgtgcagc
       61 gccaacgaca ttggtcgatc ggcgatcgtg tcgaaggtat cgtcgtaaaa atcggctcaa
      121 atgcggtttt tctcgacctg gacggaaaac gagaagcgtt cattgaccgt gcctctcttg
      181 cggatgtcac cggacaggag gcgacgctat cggtgggaga taccatcgtg gctcgtgtcg
      241 cggaagtcgg gcggcatgtg ggaggcgtga gactggaacc gttgtcgatt cgagccgcgg
      301 ggcaacaaga gcaatcagaa cgagtcgaac tcgtggatgc cgcgggacca cgcctgtcga
      361 ccggtgctca cgtgaaaggt cagatcgtgc gtgtcgaatc gtacggtgtt ttcctgcaaa
      421 tcgaagggac cacgggtcgc gaaggacggg gtctcatccc cgcatcggag accggttctc
      481 ccaaagggac agacttacga aaaaagtttc ccctgggtac gaacctggag gccaaagtgc
      541 tcagcatcgc cgacgatggg aaaattcgtc tttcgattcg tgctctccaa gccgatgagg
      601 agcgagcttc gttcgagagc ttttccgacc attcgaacga ccatcaaggt ggattcggaa
      661 caatggccgc agcctttgcc cgcagcaaaa ataagtcgaa taaacgctga cctccgtctc
      721 ctatttctcc ttcaccgcca tgccatgtgc cccttgctcc catatcgcgc cgtaccggag
      781 tagccatgcc ccttgctcca cttcctaatc ctgaaaacat caaactgcgc ctgcgcggct
      841 tgttggtggc catgcccgcg gtcgcttttt taggctcttc gctcatggcc gccaatgccg
      901 tccaaatggc aagccttccc gtgcttccca tctcaagaag cgcctttcga aaaaccaacc
      961 gctgggttgc caatacctgg tggggttggt gcgtcaccgc tggcgaagtc attcacgaca
     1021 tttccctcgt cgtgtccggc gacgatgtcc ccatgcgcga aaacgccatc gtcatcgtca
     1081 atcatcaaga catggcagac atcacctttg tgatggcgtt cgccaaatcc aaagaccggt
     1141 tgggcgacct caaatggttc gtgaaaaaag ccatcaagat cgtgccggga gtcggatggg
     1201 gaatggtctt tctcgactgt atcttcgtgg atcgacaatg gacctcggac cgagcttccg
     1261 tgcaagctgc tttccggcgc ttttcacggg actctatccc gatatggtta gtctccttcg
     1321 tggaaggaac gcggttgacg cccgaaaaac tagttcaatc ccaacgctac gccagacaat
     1381 tgggaatcga acccctcgat cacgtgctgc tcccaagaac caaaggattc gtcgcttccg
     1441 tgcagggact ccgtgatcat gtcgatgcgg tttatgatct caccttgggc tacgaacatg
     1501 gtgtccctag cctatggcag tatatccaag gtttttctcc gcgagcccat ctgcatgttc
     1561 gacgcttccc catggcggat cttccccatg atccggaggc attggcggcc tggcttcatg
     1621 cgcggttccg tgaaaaagat gagttgttgt cccgctacta ccaaagcgga tcgtttcagg
     1681 aaaccgggtc ttgaatcatc tcttcagcat ccggttgccg tgataccccc gcccgcgacg
     1741 tatcgcatca cttcagattg atcaacgaga gctgcgtacc cgctgcgtag gcgtagctga
     1801 cataattgtc gaatccgtac accagggcca gtacgggcat
//
LOCUS       DAOSOR010000801         1831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_64058_length_1831_cov_4.003941, whole genome shotgun sequence.
ACCESSION   DAOSOR010000801 DAOSOR010000000
VERSION     DAOSOR010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64058_length_1831_cov_4.003941"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1575)
                     /locus_tag="PK710_25065"
     CDS             complement(<1..1575)
                     /locus_tag="PK710_25065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOT13077.1"
                     /translation="MKRTNLPIWPLALLLPLLAGVAFAQPKAPATPVERLRIPAARGA
                     AAPAATPNPTPPPATPATPEARHGDTTSLPQFPDEIEFQPRPDGYRVAFSLEDANLSE
                     LVRVISQLTGRRFIFGGKVRDIKATVYSPQKVTVAEAYRAFLAILETNGLTVVPHGQF
                     LKIIESPGVVNQTTPIFGTGQPVPQEERYITRLYRLNHISADEVAKLLVKFKSKEADI
                     STYPPGGLLIITDTGSNVRRLLRIVEEIDVGGASNQIWVENIHYAVASELATKLGEIF
                     DISGKGQPTDAVDASDTRISKIMAEDRTNSLIIVATESAYLRVLELIKRLDVQQTGDG
                     DIHVLALQHAMAEELAQTLNNILSGMTSTSGAKAARPAGAPGGEGVFEGTLRITADKA
                     TNSLVITSSLRDYASIRTVIDMLDRPRRQVFIEAVILDVSVDHTNQFNINYHGGAAPN
                     VGGGGDSLVYGGFNPLTSILLPSADNLQGLALGVRGPDIDGTTNLLGTGLTVPAFGVV
                     VNALATSSDANVLATPH"
     gene            complement(1646..>1831)
                     /locus_tag="PK710_25070"
     CDS             complement(1646..>1831)
                     /locus_tag="PK710_25070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13078.1"
                     /translation="IGVSTVFVATMLLLLGSLADLAVAPASDGTTDATSVPEVSAQPL
                     APSRAPAKGVKTPKEQS"
BASE COUNT          392 a          509 c          548 g          382 t
ORIGIN      
        1 atgcggcgtg gcaagtacgt tcgcatcact gctcgtcgct aacgcattga ccacgacacc
       61 aaacgcggga accgtcaatc cagtccccaa taagttggtg gtcccatcga tatcaggccc
      121 ccgcaccccc aacgccaacc cttggaggtt gtccgcggag ggaaggagaa tcgaggtcag
      181 cggattgaac ccgccataca ccaacgaatc gccaccaccg cctacgttgg gcgcagcccc
      241 cccgtgataa ttgatgttga attgattggt atggtccacg gaaacgtcga ggatgacggc
      301 ctcgataaag acctgccgac gagggcggtc caacatatcg atgaccgttc gaatggaagc
      361 ataatcccgt aacgatgagg tgatgacgag cgagttggtg gctttgtcgg cggtgattcg
      421 caaggttcct tcgaaaacgc cttccccccc aggcgcaccg gcaggacgcg ccgctttcgc
      481 tccgctcgtc gatgtcattc ccgacaggat attgttcaac gtctgcgcca gttcttccgc
      541 catcgcatgc tgcaaggcca acacatggat gtccccatcg ccagtctgct gcacatcgag
      601 acgtttgatg agttcgagaa cgcgcaaata ggcggattcc gtcgccacga tgatcaagga
      661 atttgtgcgg tcttcggcca tgattttcga aatccgcgta tcactggcgt cgacggcatc
      721 cgtgggttgt cccttaccgc tgatgtcgaa aatctcacca agcttggtgg caagttctga
      781 agcgacggcg taatgaatgt tttctaccca gatttggttg gaggctcccc ccacgtcgat
      841 ttcctcgacg attcgcagca gacgtcgcac attcgagcct gtatcggtaa tgatgagcag
      901 gcctcctggc ggataggtgg agatatctgc ttctttggac ttgaacttga ccagcagttt
      961 cgcgacttcg tcggcggaaa tatggttgag acgataaagc cgggtgatat agcgctcttc
     1021 ttgcggcacg ggttgtccgg tgccgaaaat cggggttgtt tggttcacca ccccaggcga
     1081 ctcgatgatt ttcaaaaact gtccgtgagg tacgaccgtc aagccattgg tttccaaaat
     1141 ggcaagaaaa gctcgatagg cttcggctac cgtcaccttt tgaggggaat acacagtagc
     1201 cttgatgtcc cggaccttgc cgccgaagat gaagcgacgg ccggtgagtt ggctgatgac
     1261 gcgaacgagt tcggaaaggt tcgcatcttc gagggaaaac gcgacgcggt agccatcggg
     1321 acggggttgg aattcgattt cgtcggggaa ttgcggcagc gaagtcgtgt ccccatggcg
     1381 tgcttccggg gtggcaggcg tcgctggcgg cggggtaggg ttgggagtgg cggcaggcgc
     1441 agccgcacct ctcgctgcag gaatgcggag cctttccacg ggagttgctg gcgccttggg
     1501 ctgagcaaag gccaccccag ccaaaagggg cagcagcaga gcaagaggcc aaatagggag
     1561 gttcgttctt ttcatgagca attcgggtcc gtcccccgtg cgactacgac aatcgttcga
     1621 agagagcaat tgcggaaagc aaggctcatg attgctcctt cggggtcttt acgcccttgg
     1681 ccggtgcccg cgatggagcc agtggctgcg ccgatacctc gggcaccgag gtcgcatccg
     1741 tggttccgtc gctcgcggga gccaccgcca aatccgccaa ggaacctagc aaaagcagca
     1801 tcgtggccac gaacaccgtg gatacaccga t
//
LOCUS       DAOSOR010000802         1819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_64630_length_1819_cov_3.445578, whole genome shotgun sequence.
ACCESSION   DAOSOR010000802 DAOSOR010000000
VERSION     DAOSOR010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64630_length_1819_cov_3.445578"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..1713
                     /locus_tag="PK710_25075"
     CDS             283..1713
                     /locus_tag="PK710_25075"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOT13079.1"
                     /translation="MIRRDQRRVRWVMGGVLPVLASCGSMDPTDEPLGVTRQEIKAPL
                     PQAYCTIQVDGVGAVDTEKDYLPHLIQCENGGANLEALKAQAIAGRSVAYYAMAKDGR
                     ICDGQSCQVYSCGRTPEPIHHQAVEETSGMYLSYDDMLTYGFYVAGDKNVAPPSCVGS
                     SGATEKYVTYNEGKTGADVKQTTLGYVSYPIFGQNRGCMSQWGARCLENNKNYDYLGI
                     LRFFYGDDIQILTAPGSCVVPVGPELDAVLVDSGSDAEQDGQGGYRVCAGSSFHYWFE
                     LKNTGKASWSDAGGTAMGSAVRLGVPDDGVDPLTGVSRLSVSENDNDDVRSDGGDCHD
                     APGCSRTRFVEGEGIEATAPTKVGVISSTWRLVDEGRAWFGPTMSLTFEVVDCAGGAA
                     GAGGSGGAAGMGGTAGESGADAAAGASAGGGQPTPVPAATWYETEEPEGCACGIVGTR
                     KMSALWLVLLGGWLGWRRRREVRLST"
BASE COUNT          365 a          438 c          621 g          395 t
ORIGIN      
        1 cgcggtgggc cttttggggt tttcttgggg ataccttctg tcgacatggc gacggacgct
       61 gagaactgac cgttaacgga tagttggcca tgtcccttgg atgcgatgga tcgacgcctc
      121 cgaggagctt cgccgggctc tcggatgaat gcgcggccac ggtcactctg gtcgtccgag
      181 cctccggata ctttgagccg accgcagttc atgaaagttt tccgcgcggg cgaatgccgt
      241 gccggttggt atccgccggt tcgatccgct acaatgtttt tcatgattcg acgagatcaa
      301 cggcgcgtgc gatgggtgat ggggggtgtg cttcccgtat tggcgagttg tgggtcgatg
      361 gaccccacgg acgagcccct gggcgtcacg cgtcaggaaa tcaaagcccc tttaccgcaa
      421 gcctattgca cgattcaggt cgatggggtt ggcgcggtgg acaccgaaaa ggattaccta
      481 cctcacctca tccaatgcga aaacggcggc gccaatctcg aggcgctcaa agcacaggca
      541 atcgcgggcc gttccgtagc ctattacgcg atggccaaag acgggcgaat ctgtgacggc
      601 cagtcttgcc aggtctattc ttgcggaagg actcccgagc ccattcatca tcaagccgtc
      661 gaagaaacgt cgggcatgta cttgagctat gacgatatgc tgacgtacgg attttacgtg
      721 gcgggggata aaaacgtcgc tccgccaagc tgcgtgggta gcagtggagc cacggaaaaa
      781 tatgtaacgt acaacgaggg caagacggga gctgatgtca agcagacgac cctcggctac
      841 gtcagctatc ccatttttgg gcaaaaccgt gggtgcatga gccaatgggg cgcgcgatgt
      901 ttggaaaata ataagaatta cgattactta ggtatattgc ggttttttta tggtgacgat
      961 atccaaatcc tgactgcccc cggttcgtgt gtcgtgcctg tggggcccga gctggatgcg
     1021 gtgctcgtgg attccggttc cgatgcagag caagacggcc aaggggggta tcgtgtttgc
     1081 gcgggatcgt cgtttcatta ttggttcgaa ttgaaaaaca cgggcaaggc gagttggtcg
     1141 gatgcggggg gaacggcgat gggtagtgcg gtgcgtcttg gtgttcccga cgatggggtt
     1201 gatccgttga ccggggttag tcgtttgagc gtatcggaaa acgacaatga tgatgttcgg
     1261 tccgacggcg gggattgtca cgatgcgcca ggatgcagcc ggacacgttt tgtcgagggc
     1321 gaggggatcg aagcgaccgc gccaacgaaa gtgggtgtca tttcgagtac ttggcgattg
     1381 gtggacgagg ggcgggcatg gtttggacct accatgtcct tgacgtttga ggtggtggat
     1441 tgcgctgggg gggcagcggg ggcgggaggc tctgggggag cggcgggaat gggaggaacg
     1501 gcgggcgagt cgggtgccga tgcggcggca ggggcgtcgg cgggcggcgg gcaacctaca
     1561 cccgttcccg ccgcaacctg gtatgaaacc gaggagccgg agggttgtgc ttgtggcatc
     1621 gtcggaacca gaaaaatgag cgctttatgg cttgttctgc tgggcggttg gctagggtgg
     1681 cgaagacgcc gcgaagtccg attgtcaacg tgacaacacc tgctttccca cgaatactgc
     1741 ccaattaggt caatctcgca ccatcggtac gcttgtcgct cgttgggatc ggaaacggtt
     1801 gacaacttga cataacgaa
//
LOCUS       DAOSOR010000803         1813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_64935_length_1813_cov_3.610353, whole genome shotgun sequence.
ACCESSION   DAOSOR010000803 DAOSOR010000000
VERSION     DAOSOR010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64935_length_1813_cov_3.610353"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="PK710_25080"
     CDS             complement(<1..989)
                     /locus_tag="PK710_25080"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOT13080.1"
                     /translation="MSIPPLPNTKTHVLGGKVVAAGGQARQAIAALFGFDPSEATAVA
                     WVDHAVEIAIDANGNVQVLRVERRESGSRGLVRTAHLNIYARGKDLPQSFAERVRRAA
                     ERLSGWTVDHLAVLVASDPESGSPGLAMPPARDEAKRPRSLLDTWGAADSFADFFAGG
                     EIARSQLDSIDPSKLFHFVQHCDAECLFVNPHSVGFIVTLVNFPWDDRVREPGHPGGQ
                     NLANITDRDFVEEGMVTTDLTEDDVIMGNPGKLTSLIEYAVSRPSSGDKLLFVSNTCV
                     PTVIGEDVESVVKRVRNRTGKEILYLTVTPRSMTNVFQSLLVDRRLEAEAKAV"
     gene            complement(1087..>1813)
                     /gene="argS"
                     /locus_tag="PK710_25085"
     CDS             complement(1087..>1813)
                     /gene="argS"
                     /locus_tag="PK710_25085"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOT13081.1"
                     /translation="LYVVGAPQRQHLSMVFSAATMAGWLCPPARAEHVEFGSVLGADG
                     KMFKTRAGDTIKLSDLIQEGVERALAEVTLRFPELDDETRRRVARQVGVGALKYADLS
                     NDRVKDYVFDFDRMLKFEGNTGGYIQYAHARCRSILRHARLRPEPGPIEIVHPAERTL
                     ALVSLRLGAVIHQVAETLQPHTLCSYLFELAGALSGFWHDCPVLKADSELVRDSRLRL
                     VELTSRILDHGLGLLGIQAPERM"
BASE COUNT          430 a          604 c          427 g          352 t
ORIGIN      
        1 tcaaccgctt tcgcttccgc ttccaatcga cgatccacca aaaggctttg aaatacattc
       61 gtcatcgaac gaggcgtcac cgtcaagtac aggatctctt tgcccgttcg attccgaacc
      121 cgtttgacga ccgattccac atcttcccca atcaccgtcg gcacgcaggt attgctcacg
      181 aagagcaact tgtcccccga ggaaggacga gacaccgcgt actcgatcaa tgaagtcagc
      241 ttgcccggat tgcccatgat gacgtcgtcc tccgtcagat cggtcgtcac catcccttcc
      301 tccacgaaat ccctgtccgt gatgttcgct aggttttgtc cccccggatg tcccggttcc
      361 cgtactcgat catcccatgg gaaattgacc agcgtcacaa tgaaccccac cgagtgagga
      421 ttcacgaaca aacactccgc atcacaatgc tgtacgaagt gaaatagctt ggacggatca
      481 atggaatcga gttggcttcg cgcgatctcc ccgcccgcaa aaaagtccgc gaaactgtcc
      541 gctgctcccc acgtatccaa caacgatcga ggacgtttcg cctcgtctcg agcgggaggc
      601 atggcaagcc ccggcgagcc cgattccgga tcggacgcta ctaggacagc cagatgatcg
      661 acggtccaac ccgaaagccg ttccgccgct cgccgaacgc gttcagcgaa cgattgaggt
      721 aggtctttgc ctcgcgcata aatgttgaga tgcgccgtgc gaacgagccc tcgagaccca
      781 gattcccgac gttcgacacg caatacctgc acgttgccgt tcgcatcgat ggcaatctcg
      841 acagcgtgat cgacccacgc aaccgctgtc gcttcgctcg gatcgaatcc aaatagcgcg
      901 gcgatggctt gtcgtgcttg cccgcccgcc gcaacgactt ttcctccaag gacgtgcgtt
      961 ttcgtgttgg gaagaggtgg aatggacatc ggatccatcg agtggtcgca cgagcaagcc
     1021 caaggagcaa cccatcatcg cttcaaccca atacaacccg acaaaccaac gaccccgacg
     1081 aacgtttcac atccgctcgg gtgcctggat ccccagcaaa cccaacccat ggtcgagaat
     1141 ccgcgacgtc aactcgacca gccgaagccg ggaatcgcgg accagctcgg aatccgcctt
     1201 caacactgga caatcatgcc aaaagccact caacgctccc gccaactcga atagataact
     1261 gcacaaggta tgaggctgca acgtctccgc cacctgatga atcaccgcgc ccaatcgcaa
     1321 cgatacaaga gccagcgtac gctccgcggg gtgaacgatt tcgatgggcc caggttccgg
     1381 tcgtagtctc gcatgacgca agatggaacg gcatcgcgca tgggcatact gaatgtaacc
     1441 gcctgtattt ccttcaaatt ttagcattct atcgaaatcg aagacgtagt cttttacccg
     1501 gtcgttggat aaatcagcgt acttcaacgc ccccaccccc acctgccggg ccactcgtcg
     1561 acgtgtctca tcatccaact cgggaaagcg caatgtcact tccgccagcg cgcgttccac
     1621 cccttcttga atcaaatcgc tcaacttgat cgtatcgccc gcacgcgtct tgaacatttt
     1681 tccatccgcg ccaagcacgg aaccaaactc cacatgctcc gcacgcgcgg gaggacaaag
     1741 ccatccagcc atcgttgccg cagaaaatac catcgacaag tgctgccgct gcggcgcccc
     1801 caccacatac aac
//
LOCUS       DAOSOR010000804         1796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_65773_length_1796_cov_3.979322, whole genome shotgun sequence.
ACCESSION   DAOSOR010000804 DAOSOR010000000
VERSION     DAOSOR010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65773_length_1796_cov_3.979322"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..>1796
                     /locus_tag="PK710_25090"
     CDS             136..>1796
                     /locus_tag="PK710_25090"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOT13082.1"
                     /translation="MRSLRWAILGMTLIPIGCWDSEQQPQNSSAADAGQEQSESDATI
                     DNTIDNDEQASGTLRGSVKLFGKQEHSGILVQVDGSSLSTTTNNDGDFSLEGVPAATV
                     SLSFSFPSYQPEHVEGVVITENQTLTLDPVVLRLGRKMRAGEGVVPFGYGRDDAYALY
                     FSDFSPFLGSGSLWVQDLESSLEFDMGDGVSPATVFPSPDGKYLTVVHDVALGTGTGT
                     LVGLRFGDDEVVEISQEAWGGAVGYVQNGAKLIYLANVDLMRGAGDLHLVDGSTWQDT
                     VLGQNVVIDSVELTENESALWFASNPSSTDYTADLYHYAFGSQKAEKVDSKVALGVRY
                     SVGDADSIVYLKNPGTECRGTLAHWAPNSPSKTLGSKVPCPEVHVSGDSKNVLFLSDY
                     DDNSDEGVLQHWSPASANPTAVAQHVHASMELAPNGSVLVYRQPSPSPDPNVSAWDFQ
                     TSKNHSLGIVSFYLFSENSQRLVFLADTDETTELGELQTIEFATEKLASIATEASRWG
                     LEIDPSGRWIAYQRNTKDFKGDLWLFDTSDDHNEMLLEKALPSIR"
BASE COUNT          399 a          510 c          474 g          413 t
ORIGIN      
        1 aagaccatcg tgcttcggaa ttctaaatat caccaattgt tccggaattt ttcttgacag
       61 cgcgaacttc tatgatcaag ttcgtgtcca ctttttgttg caatgcacaa tgtgcctctt
      121 tcgaggagaa gcaagatgcg ttcgttacgt tgggcaatcc ttggcatgac ccttattccc
      181 atcggttgct gggattcgga acagcagcct caaaatagct ccgccgcgga tgctgggcag
      241 gaacaatccg agtcggatgc aaccatcgac aacaccatcg acaacgatga gcaggcctcc
      301 gggaccctgc ggggctccgt aaaactcttt ggaaaacaag agcatagcgg aatcctggtg
      361 caagtcgacg gctcctccct gtccaccacg accaataacg acggggactt ttccctcgag
      421 ggcgtccccg ccgcaaccgt atccctctcg ttttcgtttc cgtcctacca accggagcac
      481 gtcgagggag tcgtcatcac cgaaaatcag actctcactt tggatccagt ggtgcttcga
      541 ttaggaagaa aaatgcgggc aggcgaaggc gtggtcccct ttggatatgg ccgggatgac
      601 gcttatgctc tgtattttag tgatttctcg ccctttttgg ggtccggctc cctgtgggtg
      661 caggatctcg agtcgtccct ggagttcgat atgggtgacg gggtatctcc cgcaaccgtc
      721 tttccaagcc ccgacggcaa gtatttgacg gtcgttcacg atgtggcgct gggtacggga
      781 acaggaacgc tcgtggggct gcgtttcggc gatgacgaag tcgtggaaat ttcccaggag
      841 gcttggggcg gggccgtcgg atatgtccaa aacggcgcaa aactcatcta tctagccaac
      901 gtggatctca tgcgtggggc aggagacctg catctcgtcg atggctcgac ctggcaagat
      961 acggtgctcg gtcaaaacgt cgtcatcgat tcggtggaac tcaccgaaaa cgaatccgcc
     1021 ctctggttcg cctccaatcc atcgtccacg gactataccg cagacctgta tcactatgcc
     1081 ttcggaagcc aaaaagctga aaaagtcgat tcgaaggtgg cgctcggggt gcgctatagc
     1141 gtgggcgatg ccgattctat cgtgtacttg aaaaatccgg ggaccgagtg ccggggcacc
     1201 ctggcccact gggcgccaaa ttccccttcg aaaacccttg gttccaaggt gccttgcccc
     1261 gaggtccacg tgagtggcga tagcaaaaac gtcctatttc tctcagacta cgatgataac
     1321 tccgacgaag gcgtgctgca gcattggtcc cctgcttccg ccaatcccac cgccgtcgca
     1381 cagcatgtcc acgcctcgat ggaattggcc ccgaacggct ccgtccttgt gtatcgacag
     1441 ccttcccctt ctcctgatcc caatgtttcg gcatgggact tccaaacttc aaaaaatcat
     1501 agtcttggaa tcgtgtcctt ttatctgttc agtgagaaca gccagcgcct tgtcttcctt
     1561 gccgacaccg acgaaaccac tgagctaggt gagctgcaaa ctattgaatt tgctacagaa
     1621 aaacttgctt ccatcgcaac ggaggcgtcg cggtggggac tggaaattga cccctcgggc
     1681 cggtggatcg cttatcaacg caataccaaa gacttcaagg gcgacttatg gctattcgat
     1741 acgtccgacg atcacaacga aatgctcctg gaaaaggcgc tcccctcgat ccgatg
//
LOCUS       DAOSOR010000805         1781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_66558_length_1781_cov_3.493627, whole genome shotgun sequence.
ACCESSION   DAOSOR010000805 DAOSOR010000000
VERSION     DAOSOR010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66558_length_1781_cov_3.493627"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PK710_25095"
     CDS             complement(<1..299)
                     /locus_tag="PK710_25095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOT13083.1"
                     /translation="MDTTPRDTAPFARIDSFVAATRTAIHVRRQDRLLMIRPEKTLGI
                     NDSAVEILSALYDPKAGPSHGVVQKLASRYGVEVDRIASDAAALLSGGRFPSVRIP"
     gene            complement(299..>1781)
                     /locus_tag="PK710_25100"
     CDS             complement(299..>1781)
                     /locus_tag="PK710_25100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13084.1"
                     /translation="GVITDDQNKRLQDHFAFVYSDYQDSRRLTVYTEKKSLPAQGVHM
                     VRIYIPQSSMPNLSKTSNTRPDATIPAIPTDTGSPATSAPVTPRDVPISFGDYAARAI
                     VLAILSFFLNPIALFAEFLVLGLPLWIIDKIAKTTLLRKIFLPANEFKFAHPTKGTIL
                     GWTAVLVPFRLTWAIIAGLGSGVVALLRLHPIIELYIKKKAPVYESPEITVSTFRKDG
                     YVPTLAPEEAAVVMDRPIKAINLLVLNLQARGAIQVLSRNPLQVQVVDASRATTEPEK
                     CFLKSLTGRGTLTQQGMLEVLKSLTASVQPKIWRADTAATVEAYRQRAQQRWDELRAA
                     PPTSRPQFFNTWYADLYLQDTFFQDLGTTFGAIPTSVPADRPLTTLGAAREQSSLWGG
                     VERVQDFAEGVADTIESGAKATIDRIENLFNFDGEAGARPGIQKGVAVAVGYDACHSA
                     CHSACHSACHSACHSACHSACHDACACHSACHSACVSSCAGSF"
BASE COUNT          427 a          479 c          521 g          354 t
ORIGIN      
        1 gggattcgaa cggagggaaa tctgccgcca gaaagcaaag cggcggcatc ggaagcgatc
       61 cgatccactt cgacgccata gcgggaagca agcttttgga cgaccccgtg ggacgggcca
      121 gctttggggt catagagcgc ggaaaggatt tccaccgcgg aatcgttgat accaagggtt
      181 ttttcgggcc ggatcatcag cagacgatct tgtcgtcgca cgtggatcgc cgtgcgggtg
      241 gcggctacga aactgtcgat ccgagcgaat ggagcagtat cccgtggggt ggtatccatc
      301 agaaactccc tgcgcagctc gagacacagg cgctatggca agcggaatga caggcgcaag
      361 catcgtgaca ggcgctatgg caagcggaat gacaggccga atggcatgca gagtggcaag
      421 cagagtggca agcagagtgg caagcatcgt aaccgaccgc aaccgccaca cctttttgga
      481 taccgggacg tgcacccgcc tcgccatcga agttgaacag attctcgatc cggtcgatgg
      541 tggctttcgc ccccgattcg atggtatccg ccacgccttc ggcaaaatcc tggacccgtt
      601 ccacaccacc ccatagggaa gattgttccc gagcagcccc aagggtcgtc aggggacgat
      661 cagcaggaac gctggtggga atggccccaa acgtcgtgcc taagtcctga aaaaacgtgt
      721 cttgaaggta gagatcggca taccatgtat tgaaaaactg cgggcgagag gtgggaggag
      781 ccgcgcgcaa ctcatcccaa cgctgttgcg cgcgctgccg ataagcctcg accgtcgccg
      841 cggtatccgc acgccatatt ttgggctgca cggaagcggt caggctcttg aggacctcaa
      901 gcattccttg ctgggtcaag gttccccgcc cggtcaatga cttcaaaaaa cacttttccg
      961 gttccgtcgt cgcccgagac gcatccacga cttgcacctg caagggattg cggctcaaca
     1021 cctggatggc ccctcgggct tgaagattga gaacgagcaa attgatggct ttgataggac
     1081 ggtccatcac caccgccgct tcctcgggag ccagcgtggg cacatagccg tcctttcgaa
     1141 aagtagatac cgtaatttca ggggattcat atacaggcgc ttttttcttg atgtataatt
     1201 caatgatcgg atgaagccga agaagagcaa ccaccccgct gcccaacccc gcaataatcg
     1261 cccacgtcaa ccgaaaaggc accaggactg ccgtccagcc caaaatcgtg cctttcgtgg
     1321 gatgcgcaaa cttgaattcg ttagcaggca gaaatatttt acgaagtagt gtggttttag
     1381 cgattttatc gatgatccac aacggcaggc ccagaaccaa gaactcggca aacaatgcaa
     1441 ttggattgag aaaaaatgat agtatcgcta gaacgatggc gcgagctgcg tagtccccga
     1501 aagaaatggg tacgtctcgc ggcgtgaccg gggctgacgt cgcgggggac ccggtatccg
     1561 ttggaatggc ggggatggtg gcgtcaggac gcgtattgct cgtcttcgat aggttcggca
     1621 tcgaagattg cgggatgtag atccgcacca tgtggacccc ttgcgccggg agcgattttt
     1681 tttcggtata taccgtgagc cgccgcgaat cctgataatc ggaatacacg aaagcgaaat
     1741 gatcctgcaa acgtttgttt tgatcgtccg tgataacccc g
//
LOCUS       DAOSOR010000806         1781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_66561_length_1781_cov_3.304751, whole genome shotgun sequence.
ACCESSION   DAOSOR010000806 DAOSOR010000000
VERSION     DAOSOR010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66561_length_1781_cov_3.304751"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PK710_25105"
     CDS             complement(<1..263)
                     /locus_tag="PK710_25105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HOT13085.1"
                     /translation="MNPTAKRVLERDVRGVARACRLVDDRVDGYIEMLKDLYPHTGKA
                     WVVGVTGNPGAGKSTLTDRLVAYWRGEGKRVGVVAVDPTSPFSG"
     gene            619..>1781
                     /locus_tag="PK710_25110"
     CDS             619..>1781
                     /locus_tag="PK710_25110"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13086.1"
                     /translation="MMRLAPFYAHALVFGLSLVIAGCGDDLGSTSHDAPADSQAYDAA
                     SETFSEASPQDDATSSDGQTHDTLESDSEAESGEPYREPDTPPFASTVFGNAPGVIFR
                     WRSDGTSTSPGQYGGDIAHVKAERPAPFSVFPYTSFSPSQEKPAGYLGTTPITTQSVG
                     FTIQAWFRPRSIDGTVTLFSNTENGQGLCFDIKDGYLQMALSTSDAGKPYRHWVRDTS
                     TPLETNRWYQASVSASLKSDHWRIALFLDHNLIHEQKLPFDHGSVLVNSTELPAVAAE
                     PTGGKLDGEVFDGDMHALVVHNYPIGDDYLGTRQLREGGRTFGTPSYHDYLQIPEPGG
                     TATATPPYSDRRYNYARRVALTDNDRRFTGLRHARARLGLPFLNDRYVPNGVAV"
BASE COUNT          357 a          628 c          416 g          380 t
ORIGIN      
        1 cccgaaaatg gactcgtcgg atccaccgcc accaccccga cccgcttgcc ttccccacgc
       61 caatacgcca cgagacgatc cgtcagcgtg ctcttgcccg cccccggatt gcccgtcact
      121 ccaaccaccc aggccttgcc cgtatgcgga tacaaatcct tcaacatctc aatgtatccg
      181 tcgactcgat cgtccacgag acgacaggct cgggccacgc cgcgaacgtc tcgctccaac
      241 actcgcttcg ctgtgggatt catgagcccc tcggtagcgt caggcactac aaaaacgcaa
      301 gaaaagatgt accccaacgt ccttcggaaa ccacgctgcc gcgatccccc atgtctcagt
      361 gggtacctgc gctgcgcacc ccatcactcc tctttccccc agggcaacac aacatcatca
      421 ataaatacca atagttatgc ttattattcc cgatcgctgc tccccctgcc cttccccttc
      481 ctcccttcca ccaccccttt gtctgcccca ccccacgaat tctcaggccg tttcgtcttc
      541 ctacccgcgt tggaaaaaca atcattggtg atttgaccga atcgacgctg gctcgggtgt
      601 tgcttcccgt cccaggcgat gatgcgcctt gccccgttct atgcccatgc tctcgtcttt
      661 ggactttccc ttgtcatcgc tggctgcggc gacgatctcg gttcgactag ccacgacgcc
      721 cctgccgact ctcaggctta tgacgcggct agcgagacct tcagcgaggc gtccccccaa
      781 gatgacgcca cttcctctga tggccaaacg cacgatacgt tggaatctga cagcgaggcg
      841 gagtctggcg agccttaccg tgaacccgac acgccgcctt ttgcatctac ggtattcgga
      901 aacgctccgg gcgtcatctt tcgctggcgt tccgatggga cgtccacgag ccctgggcaa
      961 tatgggggcg acatcgcgca cgtcaaagcc gaacgcccgg cgcccttcag cgtgttccct
     1021 tatacgtctt tcagtccttc ccaagaaaaa cccgctggct acctcggaac gactcccatc
     1081 accacccaat ccgtcggctt caccatccaa gcctggtttc gccctcgatc catcgatggc
     1141 acggtcacgc ttttttccaa tacggaaaac ggccaaggct tgtgtttcga tataaaagac
     1201 ggatacttac agatggccct cagcacttcc gacgcgggga agccttatcg tcattgggtg
     1261 cgcgacacct ctacccccct cgagacgaat cgttggtatc aggcgtctgt ttccgcttct
     1321 ttgaaaagcg accattggcg aatcgctctc tttctggacc acaatctcat ccacgaacaa
     1381 aaactacctt tcgatcatgg ttcggtgctc gtcaacagca cggaactgcc ggcggtagcc
     1441 gcggaaccca cggggggcaa gctcgatggg gaagttttcg atggcgacat gcatgccctg
     1501 gttgttcata actacccgat tggagacgat tatttgggta ctcgccagct tcgggagggt
     1561 ggccgcacct tcggcacgcc cagctatcac gactacttgc agattcccga acccggcggc
     1621 actgcgaccg caaccccgcc atattccgac cgccgataca attacgcccg ccgcgtggct
     1681 ctcaccgaca atgaccgtcg attcacaggg cttcgacacg cgcgagcacg cctaggcctc
     1741 ccctttctca acgatcgcta cgtccccaac ggagtggccg t
//
LOCUS       DAOSOR010000807         1779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_66674_length_1779_cov_4.748840, whole genome shotgun sequence.
ACCESSION   DAOSOR010000807 DAOSOR010000000
VERSION     DAOSOR010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66674_length_1779_cov_4.748840"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PK710_25115"
     CDS             <1..684
                     /locus_tag="PK710_25115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13087.1"
                     /translation="VSEAGGVISMDASIGEVDAPVLGVRYWFNDGIGVDVGLGIRRWT
                     GSAKWKRYERVPGDPEAAPREFSFEQDGVSQTGFLLHAGLPIAFAKEKHYVFELVPEA
                     NIGFSGGTVKDQAQRLPSADRDPVVRDDIKLSGFRLDIGARVGSEIHFGFMGIPNLAL
                     QASVGLMLSMQKIKADGGARPPADSSTTVSDAQPGTTFQASSMEISTTVRDTPWAIFE
                     NTVSALYYF"
     gene            complement(889..1770)
                     /locus_tag="PK710_25120"
     CDS             complement(889..1770)
                     /locus_tag="PK710_25120"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HOT13088.1"
                     /translation="MAYGLFLGCNMPAIRPDVEKAIRLTMPALGVELLDMEGYVCCPA
                     FGTFPSLDEEAHLAVSAWNLSIGEKKGVDILVECGSCYRSLRSGKRCLDQHEAHRKRA
                     NELLAKTGRSYEGKATPRHISDVLLHEVGLSTIRAAVKKPLGGLRAVVQYPCHTLFPH
                     KTVGFEESSVRPHGLADLVEALGASVEHFSAEYQCCGGAGGFHGSSKAEANAFAKTKL
                     DAIIAETRADLIVVSCITCLMHLDNVQKELSRDGASYAIPVFDYNQLLALCMGFDAQQ
                     VASLCTVPRQAIVNALR"
BASE COUNT          362 a          480 c          540 g          397 t
ORIGIN      
        1 gtgtcggaag cgggaggtgt gatttcgatg gatgcgtcca tcggagaagt cgatgctccg
       61 gtgctggggg tgcgctattg gttcaacgac ggcatcggtg tcgacgttgg gttgggaatt
      121 cgtcgttgga ctgggtctgc caagtggaag cgctacgagc gggttcccgg ggatccggaa
      181 gcagcgcctc gggagttttc cttcgagcaa gacggtgtga gccaaacggg cttccttctg
      241 cacgcggggc ttcccatcgc ctttgccaaa gaaaaacact acgtattcga acttgtgccg
      301 gaagccaata tcggcttttc cggcggcacg gtgaaggatc aggcccagcg gcttccttcc
      361 gcggaccggg atcccgtcgt tcgcgatgat atcaagttga gcggctttcg tttggatatc
      421 ggagcccggg tgggttcgga aattcacttc ggattcatgg ggataccgaa tcttgccttg
      481 caggcgagcg tggggttgat gttatccatg cagaaaatca aagccgatgg tggggctcgc
      541 ccccccgcgg attcttccac gaccgtctcc gacgcgcagc cgggtacgac gtttcaggct
      601 tcgtccatgg aaatatccac caccgtccga gataccccct gggcgatttt cgaaaatacg
      661 gtcagcgcct tatactactt ctaattgccg aataaacacg caacgttgtg tgaatcgtgg
      721 ccgtcctggc ttggatgatc atggctatgt acgcgcaaca tttggtgtat tcctcgttgg
      781 ccaccgaatg cgttggctcc ccctcgaaag gattttctcc gttcccagcg caggttcgac
      841 caatattcga gacacaacgt tatgtgtaat aacggcctgt ggcttctgtt agcgcaaggc
      901 gttcacaatc gcctgacgag gcaccgtgca gagggaggcg acctgctggg catcgaaccc
      961 catgcaaagc gctagcagtt ggttgtagtc gaaaacggga atcgcatacg aggcgccgtc
     1021 gcgactcaac tccttttgga cgttgtccag atgcatcaag caggtgatgc aggataccac
     1081 gatgaggtcc gcgcgggttt cggcgatgat ggcgtcgagc ttggttttcg cgaacgcgtt
     1141 ggcttcggct ttggaagaac cgtgaaaccc tcccgccccg ccgcagcatt gatattccgc
     1201 gctgaagtgt tcgaccgaag cgccgagggc ttcgaccagg tcagccaagc cgtgggggcg
     1261 tacggagctt tcctcgaaac cgacagtttt gtgggggaaa agggtatggc aggggtattg
     1321 aacgaccgct cgcagtcctc cgaggggctt tttgaccgcg gcgcgaatgg tggaaagtcc
     1381 gacctcgtgg agcaaaacat cgctgatatg ccgcggggtt gcctttcctt cataagagcg
     1441 tccagttttg gcaagcagtt cgttggcgcg ctttcgatgc gcctcatgct ggtcgaggca
     1501 gcgtttccca ctgcgcaagg agcgataaca agagccgcat tccacgagga tatcgactcc
     1561 ttttttctcc ccaatggata gattccacgc gctcacggcc aaatgcgcct cttcgtcgag
     1621 cgaggggaac gtaccgaatg cggggcaaca tacatatcct tccatgtcca gcagctccac
     1681 accaagcgcg ggcatggtca gacgaatggc cttttcgaca tcgggccgga tagcgggcat
     1741 gttgcaaccg agaaataaac cgtaggccat gggtcagtc
//
LOCUS       DAOSOR010000808         1756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_67873_length_1756_cov_5.489124, whole genome shotgun sequence.
ACCESSION   DAOSOR010000808 DAOSOR010000000
VERSION     DAOSOR010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67873_length_1756_cov_5.489124"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..1191)
                     /locus_tag="PK710_25125"
     CDS             complement(43..1191)
                     /locus_tag="PK710_25125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOT13089.1"
                     /translation="MIRLVADLRSDGPLAVGLDHPWMRKRVSISTMSKVQVKMLAEEG
                     RFGGKNLVSSARTRVVAVTGAGSFLGKNLIGLLEEDPRVARIVALDIRPPSTAGVKTR
                     YYEVDFSHATSEQRVAEIFSAERVQSLVHVAFLSHPTHATGWAHELESVGTMQVLHAA
                     RQAGVSKLVHWSQTLLYGARPSNPNFLSEDHPLLAPADYSFFHDKIEAEKEAARFAQH
                     PDTIVTILRTAPILGPTIRNYMSRFLSRRIIPTLMGFDPLWQLTHEVDAVSAFKLAID
                     KDVPGTFNIVSDGVLPLSTIIRLAGRVSLPIPHPLAYPLAGALWLAQVSNTPVGFLDY
                     LRYLCVADGQRAVERMGFRPTYSAREALMDFASAQHLRDVRLLNDGAS"
     gene            1212..>1756
                     /locus_tag="PK710_25130"
     CDS             1212..>1756
                     /locus_tag="PK710_25130"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT13090.1"
                     /translation="MSGIDEHFPQKLGPYELVRLLATGGMAEVYLARRQGPHGFTKTV
                     ALKRILPQLVQDEDFVAMFVDEARVCAQLSHPNLVQVFDFGEADGELYMAMEFVDGTN
                     CAKAVRKAASMSETVPLDATLHIILGVLRGLEYAHAAVDVNGVPLGLVHRDVSPGNIL
                     LARSGAVKLSDFGIARANDFE"
BASE COUNT          366 a          469 c          558 g          363 t
ORIGIN      
        1 cgggccacga tcgttctccg atttcgatgc gcgatcgctg gttcagctcg cgccgtcgtt
       61 caacagccgc acatcacgaa ggtgctgcgc ggaggcgaag tccatcaggg cttcgcgagc
      121 ggaataggtg ggtcgaaacc ccattcgctc gacagcgcgt tggccatcgg cgacgcataa
      181 gtagcgaaga taatccagga atccaacagg cgtgttcgac acttgcgcga gccatagcgc
      241 tccggcgagc ggataggcaa gggggtgagg aatcggcaac gagactcttc cagcgagacg
      301 aatgatcgtc gacaagggga gcacgccatc actcacgatg ttgaaggtgc cgggcacgtc
      361 tttgtcgatg gcgagtttga atgcggagac ggcgtcgact tcgtgggtga gttgccatag
      421 ggggtcgaat cccatcaggg ttgggatgat gcgacgggat aagaatcggc tcatgtagtt
      481 tcggatggtg ggtccgagga tgggagcggt tcgcagaatg gtcacgatcg tgtcggggtg
      541 ttgggcgaag cgggcggctt ctttttcggc ttcgattttg tcgtgaaaaa acgagtaatc
      601 ggcgggggcg agcaagggat gatcttcgga taggaagttt ggattcgaag gtcgcgcccc
      661 gtacagaagc gtttggctcc aatgcacaag tttggatacg cctgcctggc gagccgcatg
      721 cagcacctgc atcgtgccca ccgattcgag ttcgtgggcc cagccggtgg cgtgggtggg
      781 atgggataaa aaggcaacgt ggacgaggga ttgcacgcgt tcggcggaaa aaatctcggc
      841 cacgcgttgt tccgaggtcg cgtgggaaaa atcaacctcg taataacgcg ttttgacgcc
      901 ggccgtggag ggtggtcgga tatctagcgc gacgattcgc gccacacgag ggtcctcttc
      961 gagcaatcca atcaggtttt tcccaagaaa tgaccctgcc cccgtcactg ccaccacccg
     1021 tgtgcgcgcg gaagaaacga ggttcttccc accaaaacgg ccttcttccg cgagcatctt
     1081 gacttggacc ttcgacatcg tgctaatact cactcgtttg cgcatccaag gatggtccaa
     1141 tcccaccgcg agcggaccat cggatcgcaa gtctgcgaca aggcgaatca cgaaggcaac
     1201 cctacccgcc aatgtccggc atcgacgaac actttccgca aaagctgggg ccatacgagc
     1261 tggttcgatt gctcgccacc ggcggtatgg ccgaagtgta cctggcgcga cggcaagggc
     1321 cccacggatt taccaaaacc gttgccctga aacgaattct tccccaattg gtccaggatg
     1381 aggacttcgt cgcgatgttt gtcgacgagg cccgggtgtg tgcccagctt tcccatccca
     1441 acctggtgca agtcttcgat tttggcgaag cggacgggga attgtacatg gcgatggagt
     1501 tcgtggatgg gaccaattgc gccaaagccg ttcgaaaagc cgcttcgatg tccgagacgg
     1561 tgccgttgga tgccacgctg cacatcatcc tgggcgtgtt gcgagggctg gaatacgcgc
     1621 atgcggcggt ggatgtgaat ggtgtgcctc ttggtctcgt gcatcgcgac gtatcccccg
     1681 gcaatatcct tctggctcgt tcaggggccg taaaattgtc ggattttgga attgcgcgcg
     1741 ccaacgactt cgagca
//
LOCUS       DAOSOR010000809         1727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_69535_length_1727_cov_3.937799, whole genome shotgun sequence.
ACCESSION   DAOSOR010000809 DAOSOR010000000
VERSION     DAOSOR010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69535_length_1727_cov_3.937799"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PK710_25135"
     CDS             complement(<1..755)
                     /locus_tag="PK710_25135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13091.1"
                     /translation="MQLLGLKTSLFASALVLFMGCSSNDSAQSDGGKKKPDEQTPNAE
                     KIMTVDETLGADAATFPPFASGTGVRHAATVVGPTGNRFRLIEKELLVATSDSAQLEG
                     VLARTGGTLLETWDFSTAGDSSGLRFHAIQIDPSRTSADDLEQALSKIESLQGRIRVS
                     STAALATFAAYASEAKQGTHVFPNFLMDNTTIMDRVTQEGPGVTWFSSDYSRNPFEWP
                     YMKRGGALDIGVAEAWRIVEANVPTGTRVPVVVL"
     gene            1192..>1727
                     /locus_tag="PK710_25140"
     CDS             1192..>1727
                     /locus_tag="PK710_25140"
                     /inference="COORDINATES: protein motif:HMM:NF014662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOT13092.1"
                     /translation="MNHTRYFNSITQEVMMTADSTRRLSKDASPVDVLAAAVRLLAEN
                     VDVCMTTVIEAQGSSPATPGQKMLLGKDGTMLGTVGGGAVEREAVDRMRAILDARAAM
                     PEVQSYALAKDLGMACGGRVQLLFEPLLSADPVLLIGAGHIGAALASLLLRVGFRVIL
                     TDERPDALSPERLAALPGV"
BASE COUNT          375 a          463 c          498 g          391 t
ORIGIN      
        1 agaaccacga ccggcacccg ggtgccagtc ggcacgttgg cttccacgat gcgccaggct
       61 tccgctaccc cgatatccaa cgctcccccc cgcttcatat aaggccattc gaagggattt
      121 ctcgaatagt cggaagagaa ccaggtgacc ccagggcctt cctgcgtcac ccgatccatg
      181 atggtcgtgt tatccatcaa aaaattgggg aaaacgtggg ttccttgttt cgcttcggaa
      241 gcgtaggccg cgaaggtcgc gagggcagcg gtgcttgaaa cgcgaatccg tccctgcaat
      301 gactctatct tcgacaaggc ttgctcgaga tcatcggcgg aagtgcggct gggatcgatc
      361 tggatagcat gaaaacgaag accggatgaa tcccccgccg tggaaaaatc ccaagtttcg
      421 agcagggtgc cgcccgttcg agccagcacg ccttcgagct gtgcggaatc cgaagtcgcg
      481 acaagcagct ccttttcgat gagccggaaa cgatttccag tcggtcccac tacggttgca
      541 gcatgccgca cgccagttcc cgatgcgaac ggcggaaacg tcgcggcatc agcccccaac
      601 gtttcatcga ccgtcatgat tttttccgca ttcggcgtct gctcatcagg cttcttcttg
      661 ccaccatcgc tctgtgctga atcattgctc gaacaaccca tgaacaatac aagcgcggac
      721 gcgaacaaac tggttttcaa acccaatagt tgcatgattt gcctccggaa cgatgcaggc
      781 gagttcctgc gaaagccctg caccttgcca aaaggtttcc atccacaagt cacattggtt
      841 ttctatgtgg acagactcgg tgaaacgccc agtcggcaaa cggcgggttg gcgctcgatg
      901 aaatgaattt gacgataatc ttcgcctaat atttattatg gtaaacatgg tatttatgga
      961 tggagagaaa aaaacagaag ctaaatgccc ttggtggtga attcgtaacc tggcagtcgc
     1021 gggaacaatc ctatcccggc gatagtgtaa tctattgaat ttattgtatt ttatgatgta
     1081 gcggggagga aggcccggat gccgagtgca gccttttcgc cctggctggc gagcgtggga
     1141 cactcgaccg ggaggcgctg atgtcttaga gtcattgacc gtggcaaggc cgtgaaccat
     1201 acacggtatt tcaattccat tacccaggaa gtcatgatga ccgcggattc gactcgacgt
     1261 ttgtccaaag acgcttcccc tgttgacgta ttggctgctg ccgttcgctt gctcgccgaa
     1321 aacgtggacg tttgtatgac gacggtcatc gaagcccaag gatcgagtcc ggcaacgcct
     1381 gggcagaaaa tgcttttggg caaagacgga acgatgctgg ggacggttgg gggaggggcc
     1441 gtggagcgtg aagccgtcga ccgcatgcga gctattctcg acgcacgggc tgcgatgccg
     1501 gaagtgcaat cctacgcact ggccaaagat cttggtatgg cgtgtggggg acgtgtccag
     1561 ttgcttttcg agccgctact gagtgctgat cccgtgctgc tcattggagc gggacatatc
     1621 ggggctgcgt tggcttcgtt actcctgcgt gtgggctttc gggtcatcct gacggatgaa
     1681 cgtcccgatg cgttgtcgcc ggagcggctt gctgccttgc ccggggt
//
LOCUS       DAOSOR010000810         1720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_69922_length_1720_cov_4.945946, whole genome shotgun sequence.
ACCESSION   DAOSOR010000810 DAOSOR010000000
VERSION     DAOSOR010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69922_length_1720_cov_4.945946"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /gene="fni"
                     /locus_tag="PK710_25145"
     CDS             complement(<1..827)
                     /gene="fni"
                     /locus_tag="PK710_25145"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOT13093.1"
                     /translation="MEQDRTATRKDEHILIAQREDVGFHRTGTLLDGVQFFHDSLPDL
                     DIDAIDTSVQVMGKRLRIPLMVAGMTGGTDKARDINKRLAEQVQGYGCAMGLGSQRAM
                     LEQPALSATYEVRDVAPTMLLFGNIGVIQARDTPTAALREMSERVGADALCVHMNPAQ
                     EVVQPEGDRDFRGGLETMGRLQSELPIPIVAKETGCGVSPRTAQRLYDMGIRHVDVSG
                     SGGTSWVAVEMKRARGTKKGLGELLREWGIPTAASVVYARRVGMETIVATGGIQTGL"
     gene            934..>1480
                     /locus_tag="PK710_25150"
     CDS             934..>1480
                     /locus_tag="PK710_25150"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOT13094.1"
                     /translation="MKERKKANPSRTPTVDHEEAVSQQLPTASDPEEAFQALLADVRR
                     DVDARLAGYLDARVAETARHGADVEAMARAVRDLTLRGGKRLRAVLVLIGYLASNQHG
                     SFDPALDAGVAFELLQTYLLVHDDWMDGDERRRGGPSVPAMLREHHGSTRLGDAAAVL
                     AGDFAAALSLDVLSRLDAPPER"
     assembly_gap    1481..1490
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1491..>1720
                     /locus_tag="PK710_25155"
     CDS             <1491..>1720
                     /locus_tag="PK710_25155"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOT13095.1"
                     /translation="NLFAEIQQDVICGQQLDIAGKELDVEVYYPLKTGSYTVRGPLLL
                     GAILAGAPPEVMHALEQYARPLGVAFQMQDDLL"
BASE COUNT          326 a          572 c          436 g          376 t
ORIGIN      
        1 tccagacccg tctgaattcc ccccgtggcg acaatggtct ccatcccaac gcgccgcgcg
       61 tagaccaccg atgccgccgt gggaattccc cactctcgga gcaactcccc caaccccttt
      121 ttcgtccctc gcgcgcgctt catttcgacc gctacccacg aggttccacc ggatccggat
      181 acgtccacat gacggatccc catatcatac agtcgctgtg ccgtacgtgg ggaaactcca
      241 catccggtct ccttggccac gatcgggata ggcaattcgc tttgcagacg gcccatcgtt
      301 tccagcccac ctcgaaagtc ccgatcccct tccggttgta ctacctcctg cgctggattc
      361 atgtgaacgc aaagcgcatc tgctcccacc cgctcgctca tctcccgcaa ggcagccgtc
      421 ggcgtgtctc gcgcctgaat cacgccaata ttgccaaata acagcatcgt aggagctaca
      481 tctctcacct cataggtggc cgataacgct ggctgctcga gcatcgcccg ctgactgcca
      541 agccccatcg cacaaccata tccctgcact tgctccgcaa gccgcttgtt gatgtcccga
      601 gccttgtccg tacctcccgt catccccgca accatcagcg ggatccgcaa gcgcttgccc
      661 attacctgca ccgacgtgtc gatggcatcg atatccaaat ccggtaagga gtcatggaaa
      721 aactgtaccc cgtccaacaa agtccctgtc cgatgaaacc ccacgtcctc ccgctgggct
      781 atcaggatgt gctcgtcttt gcgtgttgcc gtccgatcct gctccaaggt ctcctccaca
      841 tcaggttttt cttgcttcga gacccgtctg tcagagcgga cgcgatgtgt cgttgacgac
      901 ggaccgaagc cgcgcatagc ctgctcaggt cccatgaaag aacgcaagaa ggccaatcct
      961 tcccgtacgc caaccgtcga ccacgaggaa gctgtttctc aacaattgcc gaccgcatcg
     1021 gatccggagg aagcgtttca agcgctcctt gctgatgttc gtcgcgatgt ggatgcccgg
     1081 ttggccggat acctggatgc tcgtgtcgcg gaaacggctc gtcatggcgc ggatgtcgaa
     1141 gccatggcgc gggccgttcg ggacttgacc ctgcgaggtg gcaaacgatt gcgtgccgtt
     1201 ctcgtgctga tcggttatct cgcaagcaac caacacggct ctttcgaccc tgctctcgat
     1261 gcgggtgtgg ctttcgaatt gctgcaaact tatctgctcg ttcatgatga ttggatggat
     1321 ggggatgaac ggcgacgcgg tgggcccagt gtccccgcca tgctgcgcga acatcatggc
     1381 tccacacggc tcggtgacgc cgcggccgtg ctcgccggtg acttcgcggc tgccctctcc
     1441 ctcgacgttc tgtcccgact cgatgccccc ccagaacggt nnnnnnnnnn aacctgttcg
     1501 ccgaaattca acaagacgtc atctgcgggc aacaactcga cattgccggc aaagaactgg
     1561 atgttgaggt gtattatcct ttgaaaacag gtagttacac ggttcggggg ccattgcttt
     1621 tgggtgccat ccttgccggt gcccctcccg aagtcatgca cgccctcgaa caatatgctc
     1681 gacccctcgg tgtcgctttc caaatgcaag atgatttgct
//
LOCUS       DAOSOR010000811         1675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_72539_length_1675_cov_4.796296, whole genome shotgun sequence.
ACCESSION   DAOSOR010000811 DAOSOR010000000
VERSION     DAOSOR010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72539_length_1675_cov_4.796296"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..>1675)
                     /gene="fusA"
                     /locus_tag="PK710_25160"
     CDS             complement(77..>1675)
                     /gene="fusA"
                     /locus_tag="PK710_25160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOT13096.1"
                     /translation="IKSIMHAGIIDLIRMKAARFDEESRGQNIVWEPIPEDDQNLAEQ
                     MHEVLVEACADFDESIMSKYLDGCLDQVTPHQIHQAIRKGTLEGKLVPVLCGSAFRNK
                     GVQMLLDAVVNYLPSPVDIPPVEGVSKDGKKKLTRKASDDEPFAALAFKLATDPHVGN
                     LTYFRVYSGRLTAGKVVYNPTRNKRERLVRILRMHACKREEIKECNAGNIYAAVGLRD
                     TRTGDTLCDEKHTIVLEQMTFPDPVIQIAIEARTKADLDRLMDSLQRLEQEDPSFRWT
                     TNEETGQTMISGMGELHLEIVVDRLKREFHVQANVGKPQVSYRETILGKGRAEGKFER
                     VVGNHNIFGHLVLEIEPVERGKGFVFENAIPVDNLPKDFIPHIEKGLEGALTRGVLAG
                     FPLVDIKARAVDATYHEVDSTPPAYEVAASIALEAACKTAGVAIMEPIMNVEVVTPDN
                     YLGDVIGDLNARRGNVTGMSQRRNLQIIDCQVPLATMFKYTTDLRSKTQGRATHTMHF
                     SHYSPVPANIQDEIIRKIRGEYSL"
BASE COUNT          352 a          499 c          422 g          402 t
ORIGIN      
        1 ggtgcccacg tttacgtgcg gcttgttcct tacgaacttc tccttcgcca tgacgattcc
       61 tctcttgcct gtcgctctac agggagtatt cccctcgaat ttttctgatg atttcgtcct
      121 gtatattcgc gggcacgggc gaataatgcg aaaaatgcat ggtatgcgtc gcgcgccctt
      181 gggtcttgga ccgaagatcg gtcgtatact tgaacatggt ggccagcgga acctgacagt
      241 cgataatttg taggtttcgc cgctgagaca tgccagtcac gtttccgcga cgggcgttga
      301 gatcgccgat gacgtcgccc aagtagttgt ccggagtgac gacttcgaca ttcatgatgg
      361 gttccatgat ggcaacccca gcggttttgc acgcagcctc gagggcaatg gaggcggcaa
      421 cttcataagc aggtggtgtc gaatccactt catgataggt ggcgtccacc gcccgcgctt
      481 tgatgtccac aaggggaaag ccggccaaaa cgccacgcgt aagcgcgcct tccaaaccct
      541 tttcgatatg cggaatgaag tctttgggca ggttgtcgac cggaatcgca ttctcgaaaa
      601 cgaatccttt gccccgttcc acaggctcga tctcgagcac caagtgcccg aaaatattgt
      661 gatttcctac gacccgctcg aactttccct ccgctcgccc cttgcccaaa atcgtttcgc
      721 gatacgacac ttgcggcttg ccaacgttgg cttgcacatg gaactcgcgc ttgagacgat
      781 ccaccacgat ctcgagatga agctctccca tccctgaaat catcgtctgg ccagtctctt
      841 cgttcgtcgt ccagcgaaac gatggatcct cctgttcgag acgctgcaat gaatccatca
      901 atcgatccaa gtcggccttg gttcgcgcct cgatggcgat ttggatcacc ggatccggga
      961 aggtcatctg ttcgaggaca atcgtgtgct tttcatcgca aagcgtatcg cccgtgcggg
     1021 tatcacgaag cccgaccgcc gcgtaaatat ttcccgcgtt gcactctttg atctcctcac
     1081 gcttgcacgc atgcatgcgt aagatacgca ccaaccgctc acgcttgtta cgcgttgggt
     1141 tgtacaccac cttccccgcg gtcaaacgcc ccgaatacac acggaaatac gtcaaattac
     1201 cgacatgagg gtcagtcgcc aacttgaagg ccagcgccgc aaaaggctcg tcgtcggatg
     1261 ccttgcgcgt aagctttttc ttgccgtcct tggatactcc ttcgaccggt gggatatcca
     1321 ccggtgaagg caagtaattg acgaccgcat caagaagcat ctgcacgcct ttgttgcgaa
     1381 acgccgagcc gcaaagcacc ggcacgagtt tcccctcgag cgtacccttg cgaatcgctt
     1441 gatgaatctg atgcggagtg acttgatcga ggcagccatc caagtacttg gacatgatcg
     1501 actcgtcgaa gtccgcacag gcctccacca acacttcgtg catctgctcc gcaagattct
     1561 gatcgtcctc ggggatcggt tcccagacga tattttggcc ccggctctct tcgtcgaacc
     1621 tggctgcctt catccgaatc aagtcgataa tgcctgcatg cattatcgac ttgat
//
LOCUS       DAOSOR010000812         1670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_72830_length_1670_cov_3.537461, whole genome shotgun sequence.
ACCESSION   DAOSOR010000812 DAOSOR010000000
VERSION     DAOSOR010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72830_length_1670_cov_3.537461"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1517
                     /locus_tag="PK710_25165"
     CDS             <1..1517
                     /locus_tag="PK710_25165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13097.1"
                     /translation="KTIRPQARTVLLIGLGTGATATALSRAGLDVVAVELDPVVIDFA
                     RRYFSYQGHAIAADGLAFAKSDSRVFDVVVIDAFDGTQGFSDLTTSQAMQVFLERRAR
                     DGLVMVRTVDKPDSAAVNALWQAWNDRFRIAMTSGINNEPQVIVVLGSQVPMIIEDGG
                     PIPLWPMHGTEQVSPAMRETLAASEQSERQVVLMGYLVRDPSGVFVVDLPHVEMGAIR
                     YVLDEPAAKAVSPLLADKTTFPTQGDIRTDGDLSGTLHEVLGGGGVKRSEVRFSPVVV
                     KVQGKARLRALVDPDWGMSSRQPRRLPTNPLLPYGGVLYELQDAHLLWSLRYSDWNAV
                     HTRRLRPLARRAAVRLTRGEIQEGLKELRSYLQEVETSLGAAALPLPLIEDIRVLRSR
                     LEALGDPPSTPPTASWTARACDRAVHSLPEEEEETVTDGALLRSSLRSCALTYYERAA
                     LRQTGDDALFSARRLLALIDDEDSATGRRLLKAIRARYGELSPLQELPEDSLSR"
BASE COUNT          296 a          437 c          546 g          391 t
ORIGIN      
        1 ccaaaacgat acgtccgcaa gctcggacag tgctgctcat cggccttggc accggtgcga
       61 cggcgacggc gctgtcgcgt gcgggcctcg atgtcgtggc tgtcgaactc gaccccgtgg
      121 tcattgattt tgcacgtcgc tacttcagtt atcaaggtca tgccattgcg gcagatgggc
      181 ttgctttcgc caagtcggat tctcgcgtct tcgatgtggt ggtcatcgat gctttcgacg
      241 gcacgcaagg gttttccgat ctgacgacct cgcaagccat gcaagtgttt ttggagcgtc
      301 gtgcccgtga tggtctggtg atggtccgaa cggtggataa gcctgattcg gcagcggtca
      361 atgccctttg gcaggcatgg aacgaccgtt ttcgcatcgc catgacttcg ggaatcaaca
      421 atgagccgca ggtgatcgtg gtgcttggat cccaagtacc catgatcatc gaggatggtg
      481 ggcctatccc gttgtggccg atgcatggga ccgaacaggt atctccagcc atgcgcgaaa
      541 cgcttgctgc ttcggagcaa tccgagcgac aggtcgtgct gatgggctat ctggtccgag
      601 acccttcggg cgttttcgtg gttgatctgc ctcatgtaga aatgggtgcc attcgatacg
      661 tactcgatga gcctgcggcc aaagctgtct cgccccttct tgccgacaaa accacgtttc
      721 ccacgcaagg ggatatacga acggatggtg atttgtcggg tacgttgcat gaggtgttgg
      781 ggggaggagg ggtgaagcgg tcggaagtac gtttttcgcc ggttgtggtg aaagtgcagg
      841 ggaaagcgcg gttgcgggcc ctggtggacc ccgattgggg catgtcctcg cgacaaccgc
      901 gtcgcctccc tacgaatccc cttcttccgt atggtggtgt gctttacgag ttgcaagacg
      961 ctcatctgct ctggtctctg cgatattcgg attggaatgc cgtgcatacg cggcgtctgc
     1021 ggcccctggc ccgtcgcgcg gcggtgcgtc tgacgcgagg ggaaatccag gagggattga
     1081 aagaattacg gtcctacttg caagaagtgg aaacgtcgtt gggagcggcg gccttgccat
     1141 tgccacttat cgaagacata cgggtattgc gatctcgttt ggaagccttg ggggatccgc
     1201 catcgactcc ccctaccgcg tcgtggaccg cgcgggcgtg cgatcgagcc gtgcatagct
     1261 tgccggaaga ggaagaagag acggtgacgg atggcgccct tttgcgtagt tccctgaggt
     1321 cctgtgcttt gacgtattac gaacgagcgg ctttgaggca aaccggggat gacgcgctgt
     1381 tttcggcgcg ccgtttgctc gcgctgattg acgacgagga ttcggccacg ggccgtcgcc
     1441 tgctcaaggc cattcgcgcg cggtatggtg aattgagtcc tctgcaagag ctgccggagg
     1501 attctctttc gcggtgaatc ataggggtgg cacccaagga gggcacggag ccgtttttta
     1561 gtggttccat ccgcatcgaa ggggaggtcg cgtagggcaa agggttatac ggttgtctgc
     1621 aacgccttcc ctttacgtcg atgaggcatg ggcctacggc ttcgtctcgg
//
LOCUS       DAOSOR010000813         1664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_73185_length_1664_cov_3.649472, whole genome shotgun sequence.
ACCESSION   DAOSOR010000813 DAOSOR010000000
VERSION     DAOSOR010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73185_length_1664_cov_3.649472"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PK710_25170"
     CDS             <1..420
                     /locus_tag="PK710_25170"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOT13098.1"
                     /translation="PVVCSDTARVIQIPRAELLAQMKQYPGLAISINQSMGDRVQALH
                     AKVEILSAGGVEARLASLLLDLAERFGDEFDDGTFIIPIALSRQELANLISTTFETTV
                     RVMSKFKRNQIIDTTAEGFVIRDFSRLRTAASSVLGL"
     gene            complement(423..>1664)
                     /locus_tag="PK710_25175"
     CDS             complement(423..>1664)
                     /locus_tag="PK710_25175"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOT13099.1"
                     /translation="SYSPDGRFIAYLRQDVPGFEADRFRLALYDRLKGTTRVLTESFD
                     AWVLDFVWAHDGKTILFQAPVQGRFPLFEVDVDSASIQRVSGIPSVESFDRSREGSLA
                     FTYSAVGDPVELYIRKKDSKETKRVTAFNRELSADYDARPVEEMWIPGVDGKKVHTFI
                     VKPHGFREKKKYPLILNVHGGPQYQWADRFRGDWQVYPAAGYVVAFPNPHGSIGYGQE
                     YTNAISKDWGGKVHKDVLAVTDALASLPFVDPQRMGVMGWSFGGYYVNWLVGQTDRFK
                     AAASMMGIFDLRSFYATTEELWFPDWDLDGPPWNNPSVYDRFSPSNFVTRFKTPTLLI
                     TGEKDFRISYTQSLAMFTALRRQNVPARLIVFPNDGHWPSTVRSMPLYYAAHLDWFHR
                     YLGGEPSRYDPEQMIRGTAFD"
BASE COUNT          386 a          434 c          485 g          359 t
ORIGIN      
        1 cccgtcgtat gcagcgacac cgcgcgagtc attcagattc cacgagcgga attgcttgcg
       61 caaatgaagc agtatccagg acttgccatc agcatcaacc aatcgatggg cgaccgcgtt
      121 caagcattgc atgccaaagt cgagatcctg agcgccggcg gcgtagaagc tcgcctcgcg
      181 tccctgctgt tggatctcgc agaacggttc ggcgatgaat tcgatgatgg caccttcatc
      241 ataccgattg ccctctctcg ccaagaactc gccaatctca tctcgacgac attcgagacc
      301 acggttcgtg tcatgagcaa attcaagcgc aatcagatca tcgacacgac cgcggaaggg
      361 ttcgtcattc gggatttttc aagactgcgt actgcggcat cgagtgtgct tggcctgtga
      421 aatcaatcaa aagcggttcc gcgaatcatc tgctcgggat catagcgact tggttcgccg
      481 ccaagatatc gatgaaacca atcgagatgt gcggcgtaat acagcggcat cgatcgcacc
      541 gtgctcggcc aatgcccgtc gttgggaaaa acaatgagcc gagcgggaac gttttgtcgt
      601 cgcaacgccg tgaacatggc caggctttgc gtataggaga tgcggaagtc tttctcgccc
      661 gtgatgagca gcgtgggagt tttgaatcga gttacgaaat tcgagggaga gaaacggtcg
      721 tacacggatg ggttattcca gggcggacca tcgagatccc aatccgggaa ccagagttct
      781 tcggtagtgg cgtaaaaaga acgcaagtcg aagataccca tcatcgaagc agcggctttg
      841 aatcgatccg tctggccgac gagccagttg acgtagtatc caccgaaaga ccagcccatg
      901 actcccatgc gctgcgggtc gacgaagggc agcgaagcca gagcgtcggt cacggcgagg
      961 acgtctttat gcaccttgcc gccccaatct ttcgagatgg cattggtgta ttcctgtccg
     1021 tacccgatgg agccgtgagg attggggaaa gccacgacat agcccgcggc tggatacacc
     1081 tgccaatctc cacggaagcg gtcagcccat tggtattgcg gtccgccatg tacgttgaga
     1141 atcaggggat actttttctt ttcgcggaag ccatgaggtt tgacgataaa cgtgtggact
     1201 tttttgccgt ccacgcccgg gatccacatt tcttcgacgg gtcgcgcatc ataatcagcg
     1261 gataattcgc gattgaaggc agtgacccgc ttggtttctt tggagtcttt tttgcgaatg
     1321 tataattcga cggggtctcc cacggcgcta taggtaaacg cgaggcttcc ctctcgtgag
     1381 cggtcgaagg attcgacaga cgggataccg gagacccgtt ggatcgaagc agaatcgaca
     1441 tcgacttcga acaaggggaa acgtccttga accggtgctt ggaagaggat ggttttgcca
     1501 tcgtgcgccc agacgaaatc cagcacccag gcatcgaagg attcggtgag tacgcgggtt
     1561 gtgcctttca gtcgatcata gagcgcgagg cggaagcgat cggcttcgaa gccggggacg
     1621 tcttgtcgta ggtaggcaat gaatcgaccg tcgggcgagt acga
//
LOCUS       DAOSOR010000814         1631 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_75267_length_1631_cov_5.653553, whole genome shotgun sequence.
ACCESSION   DAOSOR010000814 DAOSOR010000000
VERSION     DAOSOR010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75267_length_1631_cov_5.653553"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PK710_25180"
     CDS             complement(<1..215)
                     /locus_tag="PK710_25180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13100.1"
                     /translation="MSYEDDQDRQSDPGLIPGTIIAGRFRVESKLGEGGMGAVYRATN
                     IATGRWVALKAMHPEYAVKKEVVRRFMR"
     gene            complement(342..1325)
                     /locus_tag="PK710_25185"
     CDS             complement(342..1325)
                     /locus_tag="PK710_25185"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HOT13101.1"
                     /translation="MARSKSAETRTEASDSVDSQAAGVSSGPTFVVEASLAAVVTPKL
                     QTWLEDLSGAIPRVMEKQDGEAVHDLRVAIRRIRSLLRVVRPVFGRFHVTRIRQQMQR
                     VASATGQLRDEEVLAETLDALALEPALRAGMGTWLQIRQQRERELREGVIRILSARVL
                     DMPRQQLQALLILPPAPGRDKEAYRFAKRVVIEAQRRIEALRTADVADVQGMHDLRIA
                     YKRLRYAVEALSPVLPPELRAWGQVATTFQKILGNLHDHDVAMDTLRAAAELSESTRV
                     GVMQALQDRRVYYAQRYLAEVGFEVASGAVTPETTESRAGEETEKASKSGS"
     gene            complement(1328..>1631)
                     /locus_tag="PK710_25190"
     CDS             complement(1328..>1631)
                     /locus_tag="PK710_25190"
                     /inference="COORDINATES: protein motif:HMM:NF036802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13102.1"
                     /translation="KQREYLGRHGVAVLTLAIDARGRMIFPPWLSTRGVLDDMEERDV
                     LNGAVENVVKALSSWSFSEQNPSDQEVMEVAERAVQRSLDGLSSRRPMTIVHVVRA"
BASE COUNT          354 a          582 c          367 g          328 t
ORIGIN      
        1 cgcatgaacc ggcgaaccac ttcttttttt accgcatatt ccggatgcat cgccttgagc
       61 gccacccaac gccccgtggc aatattggtg gcacgataga cggctcccat cccaccttcc
      121 ccaagcttcg actccacccg aaatcggccc gcaattatcg ttcctggaat gagcccaggg
      181 tcactttgtc gatcctgatc atcctcgtaa gacatattcg accgcgccta ccatacgctc
      241 tcatggaggc tacggcggta aagccgcaga cgcaacttcc acgctaccgc aaatcacaat
      301 ccacggaagg atacgtggaa tcctacggtc cccgcccgtg ctcaagaccc cgatttcgaa
      361 gctttttccg tctcctcccc tgcccgcgat tccgttgtct ccggcgtaac ggctcccgac
      421 gccacctcga accccacctc cgcaagataa cgctgagcat aatacacgcg acgatcctga
      481 agtgcctgca tgaccccaac gcgcgtcgat tccgatagct ccgccgccgc tcgaagcgta
      541 tccatcgcca cgtcgtgatc gtgtaagttc cccaaaatct tctgaaatgt cgttgctacc
      601 tgcccccagg cccgaagttc tggcggcaat accggcgaca aagcctccac cgcatatcgc
      661 aaccgcttgt acgcaatgcg taaatcatgc atcccttgca cgtcggccac atccgcagtt
      721 cgcaaagcct cgatacgacg ctgcgcctcg atcaccacac gtttcgcaaa tcgatacgct
      781 tccttgtctc gaccaggcgc gggcggcaag atcaacaacg cttgcaattg ttgacggggc
      841 atatccagca cgcgggccga tagaatgcga atgacccctt cgcgaagctc cctctctcgc
      901 tgctgtcgaa tctgaagcca tgtccccatg cccgcccgaa gcgcaggctc cagcgccaac
      961 gcatccagcg tttccgcaag cacttcctca tcccgcaatt gccccgtcgc cgatgccacc
     1021 cgctgcatct gctgccgaat ccgtgtgaca tgaaatcgcc caaatacggg acgaaccacg
     1081 cgcaacaaac tgcgaatgcg tcgtatcgcc accctcaaat catggaccgc ttccccgtcc
     1141 tgcttttcca tgacgcgagg aattgctcct gatagatcct cgagccatgt ctgcaatttc
     1201 ggtgtgacca ccgccgccag cgacgcttcc acaacgaatg ttggtcccga cgatacaccc
     1261 gcagcctgcg aatcgactga atcggatgct tctgtccttg tttcggcact cttggagcga
     1321 gccaaggtca agccctcacc acatgaacga tggtcatggg ccgccgactc gaaagaccgt
     1381 ccaaactacg ctgcaccgca cgctctgcca cttccatcac ctcctgatcc gaagggtttt
     1441 gctctgaaaa tgaccaggag gacaaggcct tgaccacgtt ttctaccgct ccattcagca
     1501 cgtctctctc ctccatgtcg tcgagaaccc ctcgggtgct cagccaagga ggaaagatca
     1561 tgcggcccct cgcgtcgatc gcaagcgtca gaaccgccac accatgacgg cccaaatact
     1621 cccgctgctt c
//
LOCUS       DAOSOR010000815         1601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_77193_length_1601_cov_4.018111, whole genome shotgun sequence.
ACCESSION   DAOSOR010000815 DAOSOR010000000
VERSION     DAOSOR010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77193_length_1601_cov_4.018111"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="PK710_25195"
     CDS             complement(<1..946)
                     /locus_tag="PK710_25195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13103.1"
                     /translation="MTTCGPNPSRDEHREVFEFGYSVFRRRASIENDVLRLDRGARSV
                     ELRLSALVHLYLQPRNAVQVLWLAEKTDRPTGRVHKVVANATDPGLHSLVEAIVRRRP
                     EIDLRGYSSRQAFRLMKVRDTAGRMIFGLPFLLPIGIGIWLLPYLAHGLDFGEERVSA
                     MSLSQHRSYGSHNVVITGAKARLHESTEVVTSHFRRFGPAVETTRTLVPLVPPSWEPS
                     QTVPVVLEVSEMTAFEEAAIERTVKFRGIKRDILWEGLSQEDRAYLTHQAGLHLADDV
                     WLMEYRANPRYDLFVFLAGTGTALGIAAAISVGLWLQQR"
     gene            complement(943..>1601)
                     /locus_tag="PK710_25200"
     CDS             complement(943..>1601)
                     /locus_tag="PK710_25200"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HOT13104.1"
                     /translation="SRAILSDGNLRLIRAYRGLRDQPREVIRRLQEAPYDKDYFNHQR
                     QRAIDADSDVEVAVWMIYLNRTGFNGLYRENRHGRFNVPFGRHVRPLICDEPNLLACS
                     QRLQHAQILHDTFESVESRARSGDFVYFDPPYQPVSATSTFTSYTSRGFCFEDHRRLR
                     DLALRLKRRGVHVLVSNSASSHVLGLYEKHFEIHEVQALRKVNCKREKRGRITELLIR
                     "
BASE COUNT          365 a          451 c          463 g          322 t
ORIGIN      
        1 agcgctgttg cagccacaac ccgaccgaaa tcgcggcagc aattcccaat gcggtccccg
       61 ttcccgctag aaatacgaat aaatcatacc gaggattcgc ccgatactcc atgagccaca
      121 catcatcggc cagatgcaaa cccgcttgat gggtcagata cgcccggtct tcctggctca
      181 atccttccca taaaatatcg cgtttgatgc cccgaaactt gacggttcgt tcgatggccg
      241 cctcttcgaa cgcggtcatt tcgctgactt ctaacaccac gggcacggtt tgggacggtt
      301 cccaggaggg tggcaccaac ggcacgagcg ttcgggtggt ttccaccgcc ggaccgaagc
      361 gtcggaagtg agaagtcacc acttcggtcg actcgtggag cctggctttg gcacccgtga
      421 tcaccacgtt atgggagcca taggaacggt gttgggaaag gctcatggca gagactcgtt
      481 cttcgccaaa gtccaagccg tgggccaagt agggcagcaa ccaaatgccg attcctatcg
      541 gcagcagaaa aggcaacccg aagatcatcc gtccggcggt atcgcggact ttcatgagac
      601 ggaaggcctg tcgcgacgaa taccctcgaa gatcgatctc ggggcgtctt cgcacgatgg
      661 cttcgacgag cgaatgcaat cccgggtccg tggcgttggc gacgacctta tgcacccggc
      721 cggtgggacg atcggttttt tccgcgagcc acagtacctg aacggcattt cgcggttgca
      781 ggtagagatg aaccagagcg gataaccgga gttcgaccga acgagcgccg cgatccaagc
      841 gcaacacgtc gttttcgatc gaagctcgac gtcgaaacac ggaataacca aattcaaata
      901 cctcgcggtg ctcatcccgc gacgggttgg ggccacaggt cgtcatcgta tgagcaactc
      961 cgtgatgcgc ccccgtttct cccttttgca gttgaccttc cgaagcgcct gtacctcatg
     1021 gatttcaaag tgtttttcgt aaagcccgag cacatgactc gacgcggaat tcgacacgag
     1081 gacatgaacg ccacgacgtt tgagccgcaa ggcaaggtca cgcaggcggc gatggtcttc
     1141 gaagcaaaag ccgcgagagg tgtaggatgt aaacgtggag gtcgcggata cgggttggta
     1201 gggtggatcg aagtagacga agtcaccgct ccttgcccgg ctttcgacgg attcgaaggt
     1261 atcgtggaga atctgagcgt gctgcagccg ttgcgagcac gcgagcaagt taggctcatc
     1321 gcagatcaag ggtcgaacgt gacgtccgaa agggacattg aatcggccgt ggcggttttc
     1381 gcgataaaga ccattgaagc cggttcggtt caagtagatc atccaaaccg ccacttcgac
     1441 atccgagtct gcatcgatgg ctcgttgccg ctgatgattg aaatagtcct tgtcataggg
     1501 agcctcttgc aagcgtcgga tcacctcccg cggttggtct ctaagtcccc gataagcgcg
     1561 aatcaaccgt agattgccgt ccgacaagat ggcgcgcgac g
//
LOCUS       DAOSOR010000816         1592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_77816_length_1592_cov_5.055953, whole genome shotgun sequence.
ACCESSION   DAOSOR010000816 DAOSOR010000000
VERSION     DAOSOR010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77816_length_1592_cov_5.055953"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PK710_25205"
     CDS             <1..454
                     /locus_tag="PK710_25205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13105.1"
                     /translation="TSLLDRYVNEDALRTLDVKLRGTRSPYEGRAIRALLLGDGSPDP
                     RLAFTLLGKWIVEAPADGLPAYLAGRNLMNARAWMAAAESLDWALERDLHEPRIMREA
                     LRLRIIVACAVGDVATARKVMTRWKAQPELQEVRWRVLERRLGACVKQ"
     gene            567..1454
                     /locus_tag="PK710_25210"
     CDS             567..1454
                     /locus_tag="PK710_25210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13106.1"
                     /translation="MRPIRHYFLLFLPVVTTGCSTCSEKTNEPNAVASVSLPEVIAPG
                     DAVVAEVAHASFIEGSVSAVHRQTLILTDHETGKTREVDSANVYRLGKPAKTEWQIGD
                     LAICRIEPSRWRGCKITSFHANQISIVDDQGHDHTVDHRQLLVPSPVTKLNLVHAFDR
                     VERRRHFEMERQNAGDLYRPTDWKPRAGENVILRNGSHFVGATVSEARNTLLLVQSGG
                     QKPHAVAVEDVWPEPPVETTLSPKSFACLRPPTGEYVWQVVRIEAEQNDKWLVSFASG
                     EQNTVASKDLLPFVPARTR"
BASE COUNT          381 a          452 c          431 g          328 t
ORIGIN      
        1 cacgagtttg ctcgatcgat acgtcaacga agacgcgtta cgaaccttgg atgtcaagct
       61 tcgcggaacg cgaagcccct atgaaggcag agcgattcga gccttgctgc ttggagatgg
      121 gagccccgat ccgagattgg ccttcaccct gcttggaaag tggatcgtgg aagcgcctgc
      181 agatggtctg cccgcatact tggcgggtcg caatttgatg aacgcacggg cctggatggc
      241 cgctgccgaa tcactcgatt gggcgctcga gcgagacctg cacgaaccaa gaatcatgcg
      301 tgaagccctg cgtcttcgca tcatcgtagc ctgcgccgtg ggagacgttg cgacggctag
      361 aaaagtgatg acacgctgga aagcgcaacc agaactgcaa gaggtgcgct ggcgcgtgtt
      421 ggagcgacga ctcggagcgt gcgttaagca ataagaagca tcaccatacc cgcgagatcg
      481 gtggcagaca gcgacatcgg gatgtggaat tggtcttgtc tatcgcgacg aatcccatca
      541 cgatgctctt gcgagtccta cgattcatga ggccaattcg ccattatttc ttgctatttt
      601 tgccggtggt gaccacgggc tgttctacgt gttcagaaaa aaccaacgaa cccaatgcgg
      661 tagcgtccgt ttctttgccc gaggtcattg ctcccggtga tgctgtcgtg gccgaagtcg
      721 cccacgcatc tttcatcgaa gggtccgtgt ccgcggtgca tcgtcaaaca ctcattctta
      781 ccgaccatga aaccggtaaa acgcgcgagg tggactcagc caacgtgtac cgattgggaa
      841 aacccgcaaa aaccgaatgg caaatcggag atcttgcgat ctgtcgcatc gaaccctctc
      901 gctggagagg gtgtaaaata acgtcgtttc atgcaaacca gatatccatc gtagacgatc
      961 aaggccatga tcataccgtc gatcaccgtc agctactcgt cccctccccc gtaacgaaac
     1021 tcaatctcgt ccacgcgttc gatcgtgtcg aacgcagacg ccatttcgaa atggagcgtc
     1081 aaaacgccgg agatttgtat cgtcccaccg actggaaacc acgagccgga gaaaacgtca
     1141 tcctccgaaa tggaagtcat ttcgtcggag ctaccgtgtc ggaagcgcgc aacaccctgt
     1201 tgctcgtaca atcgggtgga cagaaacctc atgcggtggc ggtcgaggac gtatggcccg
     1261 aaccgcccgt ggaaacgacg ctttcaccga aaagcttcgc gtgtttgcgt ccacccacgg
     1321 gcgagtacgt ttggcaagtc gttcgaatcg aagcagagca aaatgataaa tggttggtca
     1381 gcttcgcttc gggagaacaa aacacggtag ccagcaagga ccttcttccc ttcgtccctg
     1441 ctcgaactcg ctgaccaaca tcgtctgctg caccctcgaa ggtcgacgaa gcccagtcga
     1501 tgagaaagcg tcacgtagaa aactaaagcg cggtctttcg tccttcctca tccgtccgaa
     1561 cgacgttgcc actccccatc acatcgcgaa ga
//
LOCUS       DAOSOR010000817         1587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_78178_length_1587_cov_4.537206, whole genome shotgun sequence.
ACCESSION   DAOSOR010000817 DAOSOR010000000
VERSION     DAOSOR010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78178_length_1587_cov_4.537206"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PK710_25215"
     CDS             <1..603
                     /locus_tag="PK710_25215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13107.1"
                     /translation="MAAAVLDGFAFLYPSYRSWQAIDELARRHDDRVHRQMAASRLGQ
                     AGSRRTVAQVDDALARGDLANARQFAAELHLESGQLAVRAAALGQWEAARQQARMVLA
                     AEPGHPDATAVFLSAPSAWESFQQSDPVIWQRLLKSPENNPRRLSDLAALVLADAIRR
                     TIGMQAARSWLARWNLSYDIYHEPLAHPLLIRLGVSTEPR"
     gene            687..1184
                     /locus_tag="PK710_25220"
     CDS             687..1184
                     /locus_tag="PK710_25220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13108.1"
                     /translation="MEERTSLYLTQLSAADRKLLQLSVQMNPLMVANADGNYSLREAA
                     AVSEVVRSLMSDPQFRPLILVAGHAELSDAALRVMLETHTKDIDGYLSQLADLLEQLP
                     EDVAIAYRRFTLFAIIRVAEASRDGLFGLMGDRISQCEKSVMRKMVDALALEPNEEEK
                     TKLGM"
     gene            1215..>1587
                     /locus_tag="PK710_25225"
     CDS             1215..>1587
                     /locus_tag="PK710_25225"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT13109.1"
                     /translation="MHDTPEPQVSEQQEPDVRPSMSSLVGELLSGRYRIEAELGSGGM
                     GAVYRAEHTLMRKRVAIKVLHPEMTQHTEVVERFEREAMAAAHIEHPNVAAATDFGKL
                     ENGSFFLVLEYVEGACCATSCS"
BASE COUNT          337 a          447 c          463 g          340 t
ORIGIN      
        1 atggccgcgg cggttctcga tggtttcgca tttttgtatc cttcctatcg gagctggcaa
       61 gccatcgatg agttggctcg tcggcatgac gatagagttc atcggcaaat ggctgcgagt
      121 cgactcggcc aggctggaag ccgacgaacg gtcgcgcagg tggacgatgc cttggcgcgc
      181 ggggatttag cgaacgcacg gcaattcgcg gctgagttgc accttgaatc gggacagctt
      241 gcggtacgag cggcggcgct cgggcagtgg gaggcggccc gacagcaagc tcgaatggtt
      301 ctcgcggccg agccaggcca ccccgatgcc acggccgtct ttctttccgc ccccagtgcc
      361 tgggaatcct ttcagcaatc cgatccggtc atttggcaaa gactattgaa atcacccgag
      421 aataaccctc gtcgtctctc ggatctggca gccttggtac tggcggatgc gatacggcga
      481 accatcggca tgcaagcggc tcggtcttgg ctcgcacgat ggaacctatc ctacgacata
      541 taccatgaac ctctcgctca ccctttgctc attcgcctgg gcgtgagcac ggagccacga
      601 tgaatccgtc ccctcttcgc ttgctctgct tgtatgctgt gggaacatcg tatcttaccc
      661 atcaagccgc tttggggtga atctccatgg aagaacgaac cagcctatat ctcacccaac
      721 tcagcgctgc ggatcgaaag ctgctccagc tatccgtgca aatgaacccg ctgatggttg
      781 ccaacgcgga tggcaattac tcgttacggg aggccgcggc ggtgtcggaa gttgtgcgct
      841 ccctaatgtc ggatccgcaa tttcgcccac tcattttggt ggcaggacat gcggaattat
      901 ccgatgcggc cctgcgggtc atgctcgaaa cgcataccaa agacatcgat ggatacctga
      961 gtcagctagc ggatttgctg gaacaactcc ccgaagatgt tgcgattgcc tatcgacgct
     1021 tcaccctttt tgcgatcatt cgcgtcgcgg aagcatcgcg agacggactt ttcggcttga
     1081 tgggagatcg gataagccaa tgtgaaaaat ccgtcatgcg caagatggtc gacgcgctcg
     1141 cgttggaacc caacgaggag gagaaaacga aattgggcat gtaggtttcg tcttccccat
     1201 taacgataga acgaatgcac gacacgccag agccgcaggt tagcgaacag caggagccgg
     1261 acgttcgacc ttcgatgtcc tcgctcgtgg gcgagttatt gtccggtcgc taccgcatcg
     1321 aagctgaatt gggaagcgga ggcatggggg ccgtatatcg ggcggaacac accctgatgc
     1381 gcaaacgtgt agccatcaaa gtgctgcatc ctgaaatgac gcagcacacc gaggtcgtcg
     1441 aacgcttcga gcgcgaggcc atggcggctg ctcatatcga gcatcctaac gtggcagcgg
     1501 ccacggactt cggcaaacta gaaaacggat cgttttttct cgtcctcgag tatgtcgaag
     1561 gcgcttgttg cgcgacgtct tgcagca
//
LOCUS       DAOSOR010000818         1573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_79148_length_1573_cov_4.364295, whole genome shotgun sequence.
ACCESSION   DAOSOR010000818 DAOSOR010000000
VERSION     DAOSOR010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79148_length_1573_cov_4.364295"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PK710_25230"
     CDS             <1..666
                     /locus_tag="PK710_25230"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOT13110.1"
                     /translation="VGAWAWVRDYGLDRSAARLRQPPLTRDDFLGLITPSHLDRLNDS
                     LRTNPWRGLVVACPYTPDILGHSDLDAAVPMARFVKQYLIPKVRKRFPVIDSRQATGI
                     DGVSLGGRMALLVATLYPEQFGAVGTLQAAFRDQELPMLVQRIRWAWQQPVHPMALRI
                     LTSDHDPFRPTLEKLASMLQPAPQGFAFDLVAGPHDIDFNRGPGGIEMLRWQERVLVH
                     NKT"
     gene            998..>1573
                     /locus_tag="PK710_25235"
     CDS             998..>1573
                     /locus_tag="PK710_25235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT13111.1"
                     /translation="MDDKEPLALTRPTDEQTSAHLRIAKLEGALHDVANALTVVLGWL
                     EAAEQPSTQDASVSRAIHWARERGLHGRAIALRAMGYGKEAPEQPAVCESIVADAVVG
                     MEPEAKRKGIELFSHIDGEARLLGVTAPSSALQVLTNLLLNAIAFSPDGARVSLTVAC
                     LDTDTLRFVVTDEGPGIPPDRAGRVFDGLVST"
BASE COUNT          313 a          416 c          504 g          340 t
ORIGIN      
        1 gtgggagcct gggcttgggt tcgtgattac gggctcgacc gcagcgccgc acgtcttcgt
       61 caaccgccgt tgacccgcga tgatttcctc gggctgatca caccatcgca tctcgatcgt
      121 ctcaatgatt cccttcgtac gaatccatgg cggggcctgg tggtggcctg cccgtacacg
      181 ccggatattt tgggtcattc ggatctggac gccgcggtgc ccatggctcg gttcgtgaaa
      241 caatacttga tcccaaaggt acgaaagcgc tttccggtga tcgactcgcg tcaggctacg
      301 ggaatcgatg gggtgagttt aggcggaaga atggcattgc tggtggcgac cctgtatccg
      361 gaacaatttg gagccgtggg aacattgcaa gccgcctttc gcgatcagga gttgccgatg
      421 ctggttcaac gaattcgatg ggcgtggcaa caacccgttc acccgatggc gttgcggata
      481 ctcacgagcg accacgaccc atttcgacct accctcgaaa agctcgcttc gatgctccaa
      541 ccagccccac aagggtttgc attcgatctg gtggcgggtc cccacgacat tgatttcaat
      601 cgaggtcccg gtgggatcga aatgctccgg tggcaagaac gcgtgctggt gcataataaa
      661 acataagcaa tatcaatatg ttataaatga acggggagcc cgtccatgct ccgacggttc
      721 ctagaccatg gccgctacgt agcggggtct tcgtgaaccg gagacgaacc tgtgaagagg
      781 aaggcatgga atcggtggtg attcccgaag gcaggcggtg ttctcctgat cggctcgaac
      841 ctagtggcga agacacgaac cggtgagcag gaggggcagt cgtcttctcc ccttggcgca
      901 ggcggggatg gcaagcggtt cttgcagtta cgatcggttc gcggtacctc attgggtcgc
      961 acgagtgtaa tagtatgttc ggataggatt tgatctcttg gacgataagg agccactggc
     1021 attgactcga ccgacggacg agcagacatc cgcccatctg cgcattgcaa agcttgaagg
     1081 agccttgcac gatgttgcca atgccttgac ggtggtgctg gggtggttgg aggcggcgga
     1141 gcaaccatcg acgcaagacg catcggtttc gcgggcgata cactgggcac gagagcgggg
     1201 gctccacggg cgtgccatag cccttcgggc gatgggttac ggcaaggagg ctccggagca
     1261 gccagccgtg tgtgagtcga ttgtcgccga tgcggtggtg ggcatggagc ccgaggccaa
     1321 gcgcaagggt atagagctat tcagccatat cgatggcgag gcacgactgt taggcgttac
     1381 ggcgccatcg tcggcgcttc aggtgctgac gaatctccta ttgaacgcca tcgcgttttc
     1441 tcctgatggt gcaagggttt cccttacggt agcgtgcttg gacaccgata cgctgcggtt
     1501 tgtggtcacc gacgaaggac cggggatacc gcccgatcga gccggccgcg tttttgatgg
     1561 tcttgtctcg acg
//
LOCUS       DAOSOR010000819         1540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_81571_length_1540_cov_3.863300, whole genome shotgun sequence.
ACCESSION   DAOSOR010000819 DAOSOR010000000
VERSION     DAOSOR010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81571_length_1540_cov_3.863300"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="PK710_25240"
     CDS             complement(<1..848)
                     /locus_tag="PK710_25240"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT13112.1"
                     /translation="MPAERRPRIKRSVRALALARAQARSSRLAAIYSALSAVHASVSE
                     SKSVTDMFGRACQAIVEQHVFRGAWAGRFRNDGLLHVLAVAGSFQHSPPETLVAASPE
                     QAWVQSTSLHQTTVVRPDDPPQRRDPILDHLACSSALYVPVESDSGVLGVLCVATNDP
                     RSFDREVTAMLIELGRSLSANAGRINAEQALASQRELLRAVFDSATVAIYYATAEGKL
                     LLCNDAFLRLVGRQRDDTVGQSYQVVSSPDFREYERTIVQRVLSSGLPSEYDKDVVRS
                     DGFRIPV"
     gene            974..1050
                     /locus_tag="PK710_25245"
     tRNA            974..1050
                     /locus_tag="PK710_25245"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1008..1010,aa:Met,seq:cat)
     gene            complement(1132..1296)
                     /locus_tag="PK710_25250"
     CDS             complement(1132..1296)
                     /locus_tag="PK710_25250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13113.1"
                     /translation="MAGTWGPGCATARREQQVAADKKSRDVSMRPPRALFRIVDSPAD
                     LPEHRPLSPC"
BASE COUNT          327 a          427 c          491 g          295 t
ORIGIN      
        1 acgggaatgc ggaacccatc gctgcgcacc acatccttgt catattcgga aggcaagcct
       61 gaagaaagca ctcgctgcac aatcgtccgc tcgtattctc ggaaatcggg cgatgacacc
      121 acctggtacg attgccccac cgtatcgtct cgttggcgac ccaccaaccg aaggaaagcg
      181 tcgttgcaaa gcaacaattt gccttccgct gtggcgtagt agatggcgac cgtggcgcta
      241 tcgaataccg cgcgcaatag ttcacgctgg ctcgcaaggg cttgttcggc attgattcga
      301 ccagcgttgg ccgacagaga gcggccaagc tcgatgagca tcgcggtcac ttccctgtcg
      361 aaactccggg ggtcgttggt cgctacgcac aacacgccaa gcacgccgga atcgctttcc
      421 accggaacgt ataacgctga ggagcaggcg agatggtcga ggatcggatc ccgacgttgc
      481 gggggatcat ccggacgcac gactgtagtt tgatgcaggg atgtggactg gacccacgct
      541 tgctcagggg aagcggcgac aagagtctcg gggggcgaat gttggaaaga gcccgcgaca
      601 gccaggacat gaagcagccc atcgttgcgg aaccggcctg cccaagcgcc tcggaaaacg
      661 tgctgttcaa cgatggcttg gcatgcgcga ccgaacatat cggtgacgga tttggattcg
      721 ctgacggagg cgtggacggc ggacagcgcg gaatagatgg cggcaaggcg agagcttcgc
      781 gcttgtgcgc gagcgagagc cagggcacgg acggaacgct tgatgcgagg tcgccgctcg
      841 gcgggcatgg gccgagtgta cggcagaacg acgaaggggc ctagcgctcg ccgaaaaggt
      901 tgggaggaga gaaaaatgaa acaagcgctt gacagtggtg ggttcgggag tagattgcct
      961 gccacttcat cgacgcgcgg tggagcagcc tggtagctcg tcgggctcat aacccgaagg
     1021 tcggaggttc aaatcctccc cgcgcaacca tcgtttcctt gttgcaggag aagcctcgat
     1081 tccctttgga attcgaggtt ttttcgattt catcgacggc tgcgcaagac cctagcacgg
     1141 ggaaagaggg cgatgctccg gcaaatccgc ggggctgtcc acgatacgga acagcgcacg
     1201 cggtgggcgc atcgaaacgt cccggctttt cttgtcggcc gctacttgtt gctctcgtct
     1261 ggcggttgca cacccaggac cccaggtgcc agccatgggc atgggatagt agcgagttgg
     1321 ttcatcgttc catgagaatc actcccgcct gccgtatgct ttcgggacat cttcttgtcg
     1381 gggggtgtag attattcagg agaaagaaag agcgattgcc gtgacacgac accaatgtct
     1441 catgcccttg tacgcgctgc tgctcctggc atggcccggg tgctgccctc ctgaatttaa
     1501 aacgtcacaa ggcgattccg cggccgactc ggcagtcgat
//
LOCUS       DAOSOR010000820         1534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_82013_length_1534_cov_3.882353, whole genome shotgun sequence.
ACCESSION   DAOSOR010000820 DAOSOR010000000
VERSION     DAOSOR010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82013_length_1534_cov_3.882353"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PK710_25255"
     CDS             <1..668
                     /locus_tag="PK710_25255"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HOT13114.1"
                     /translation="SPRCGDIVVDRVFVGSGRRQLMTQSDLGGAASVTALAVSPSVSH
                     EESSKIVDSTEWREPMAYGFATRNMQGITPLDLDVSLPTTVQDGQAVSVTIRTSKKAW
                     LIVFYQESDGRAALLWPSAQEPEPVAEPNHPAVLPSSAERNAGIRIQAQLHQPDKPVH
                     ELFVVYAFSEKTDWERFKPTFGAESTEGAAYVSEITNKINDLSMARWSRAILAYTIVP
                     ASL"
     gene            695..>1534
                     /locus_tag="PK710_25260"
     CDS             695..>1534
                     /locus_tag="PK710_25260"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOT13115.1"
                     /translation="MHNLSLHFLGLVLGMSIVAGCGTPKIVKAPPNIEGLRVFVSEPR
                     LTGDAASECAALAPALRSATHSALLKAGFDVVTDMSAPHDAVANVNAEVVSHGLWPLK
                     TYETNAFLSLERDGTIIDQVEGDSGQYRSCTGYEQTVATNLVNSLSASERVARFASSR
                     GTNVAGVAQSDSPTPIVPSEFVAGAPQPSTWALVIGIERYRDVPPPTGARQDAEYFAA
                     MLQRTAGVPEDQLRLILDERATRSDIDKELAWLEKNVPAGARIIVYFSGHGTPDTAKG
                     TPYL"
BASE COUNT          332 a          430 c          428 g          344 t
ORIGIN      
        1 ttagtccccg gtgcggagat atcgtggtcg acagagtttt tgtcggttcc ggacgccgcc
       61 aactcatgac ccaatccgat ctcgggggtg ccgctagcgt cacggctctt gctgtttcac
      121 cttcggtgtc tcacgaagaa tcgagtaaaa tcgtggactc taccgagtgg cgcgagccca
      181 tggcctacgg ctttgccaca cggaatatgc aaggcattac gcccctggat ctcgacgtct
      241 cgttacccac cacggtgcaa gacggacaag ctgtctcggt gaccattcgt acgagtaaaa
      301 aagcgtggtt gatcgtgttt taccaagaaa gcgatggacg tgccgcgctg ctatggcctt
      361 ccgcgcagga acctgaaccg gtggcggagc ccaatcatcc tgccgttctc ccttcttccg
      421 cggaacggaa tgccggaatc cgcattcagg cgcaactcca ccaaccagac aaacccgtgc
      481 atgagctgtt cgtcgtctat gcgttttcag aaaaaacgga ttgggaacgc ttcaaaccaa
      541 cttttggggc ggaatccacg gaaggagccg cctatgtatc agaaataact aataaaatca
      601 atgatttgtc tatggctcgc tggtcaaggg ccatccttgc ctatacgatt gttcccgcct
      661 ccctttgatg cggtttgccc tatagataag agctatgcat aacctgtctt tacacttttt
      721 ggggttggta ctcgggatga gtatcgtggc gggttgcggt acgcccaaga tcgtcaaagc
      781 accgccgaat atcgaagggc tacgggtgtt cgtttcggaa ccgcgattga cgggtgatgc
      841 tgcaagtgaa tgcgctgctt tggctcccgc tcttcgcagc gccacgcatt cggcattgct
      901 gaaggccgga tttgacgtcg tgaccgatat gtcggcaccc cacgatgctg tggcgaacgt
      961 gaatgcggaa gtggtttcgc atggtctttg gccgctcaag acgtatgaaa ccaatgcttt
     1021 tctctctctc gaacgggacg gcaccatcat tgatcaagtc gaaggagata gcggccagta
     1081 caggagttgc acgggatacg agcaaacggt ggctaccaac ctcgtcaatt ctctgagcgc
     1141 ctccgaacgg gtggcccgat ttgcgagcag ccgggggacg aacgttgcgg gcgttgcgca
     1201 gtctgattct cccacaccga ttgtcccttc ggaattcgtg gcaggcgctc cccaaccttc
     1261 tacttgggcc ctggtcatcg gcatcgaaag gtaccgggat gttccacctc ccacgggagc
     1321 acgccaggat gcggaatact tcgccgccat gcttcaacgt acggcagggg tgccggagga
     1381 ccaacttcga ttgattctcg atgaacgcgc cacccgatcc gacatcgaca aagaactcgc
     1441 ctggctcgaa aaaaatgtgc ccgcgggtgc aagaatcatt gtctacttca gtggtcatgg
     1501 gacgccggac acggcgaagg gaacgcctta cctt
//
LOCUS       DAOSOR010000821         1505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate AMR_MDS_2340
            NODE_84280_length_1505_cov_4.348276, whole genome shotgun sequence.
ACCESSION   DAOSOR010000821 DAOSOR010000000
VERSION     DAOSOR010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864407
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (wastewater metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:30:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 5,015
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84280_length_1505_cov_4.348276"
                     /isolate="AMR_MDS_2340"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PK710_25265"
     CDS             complement(<1..282)
                     /locus_tag="PK710_25265"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOT13116.1"
                     /translation="MIDLDVAQRNLWQDIDPHGNLLYCFSCSTCVVGCPASNAIPPLL
                     IRNLVRSVTLGLEDDLLDNDTPWTCVTCSRCEEMCPMGVDPFEIVLALRR"
     gene            complement(385..615)
                     /locus_tag="PK710_25270"
     CDS             complement(385..615)
                     /locus_tag="PK710_25270"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT13117.1"
                     /translation="MQALPENVVRSCPGSLMSHESHDMDILDPIRESEKNTIVSVLLR
                     NAGNRSRAAAELGIHRSTLIRKIKRYGIDIQS"
     gene            complement(755..>1505)
                     /locus_tag="PK710_25275"
     CDS             complement(755..>1505)
                     /locus_tag="PK710_25275"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT13118.1"
                     /translation="AGSCAKATMYTLKDAPMVTTAILVTETGHVLGGVEILSDLSPLE
                     HLQRQLDGKYCFEDIISRSPKMQEIFTVLPMVANSESTVMVLGESGTGKELLAKAIHH
                     HSPRQQQPFVAVNCAAVPETLMESELFGYVRGAFTDAKRDKPGRIAQADGGTLFLDEV
                     GDLPLSIQVKLLRFLQERSYEPLGATFSTRSDVRIICATNRPLQEMVAMGTFRQDLYF
                     RLNVMQIELPPLRERIEDVPLLAQHFVQRFR"
BASE COUNT          359 a          428 c          372 g          346 t
ORIGIN      
        1 ccttcgaagc gccagaacga tttcaaacgg atcgaccccc atcgggcaca tctcctcaca
       61 acgcgagcac gttacacacg tccacggagt gtcgttgtcg agcaaatcgt cttccaaccc
      121 cagggtgacc gagcgaacca ggttgcgtat gagcaaagga ggaatcgcgt tggaggcagg
      181 gcagccgacg acacacgtgc tgcaagaaaa acaatacagc aggtttccgt gcggatctat
      241 gtcctgccat aggtttcgtt gggctacatc aaggtcgatc atcaacacgt ctccctgaac
      301 gggttcgata atgcatggac ttcgggcatt tgcagtgggt ccgtcgactt cgccgaagac
      361 gactaccccc agagtatctc gctattacga ttggatatcg attccgtacc tctttatttt
      421 tcgaatgagg gtggagcgat gaatcccaag ctccgccgcg gcgcgggaac ggtttcccgc
      481 attgcgcagc aacacagata cgatggtatt tttctccgat tcccgaattg gatccaatat
      541 atccatgtcg tgggactcat gactcatcag actgccaggg caagaacgaa ccacgttttc
      601 cggcagcgct tgcacaccaa tcctggagtc tcggcataaa agaaaagctc gttcgatgat
      661 actttttaag ttcccgaata ttccccggga agttgtatcg tgacaacatc atcatcgctt
      721 gattgtcaat cccctcgatc cggttgttgg taagtcagcg aaaacgttgc acaaaatgct
      781 gtgccaacaa ggggacatcc tcgatgcgct cgcgcagcgg cggaagctcg atttgcatga
      841 cattcaggcg aaaataaagg tcttgccgaa aggtgcccat cgcgaccatc tcctgcaaag
      901 gacgattggt ggcgcagatg attcgaacgt ccgaccgggt agaaaacgtg gccccgagcg
      961 gttcataact tctttcctgc aaaaaccgca gcagcttgac ctgaatggat aggggaagat
     1021 cccccacttc gtcgagaaag agcgttccac catccgcctg ggcaatcctt cccggcttat
     1081 ctcttttcgc atccgtaaac gcaccccgca catagccaaa aagttccgat tccattaagg
     1141 tttctggaac cgcggcgcag ttgacggcca cgaaaggttg ctgctgccga gggctgtgat
     1201 gatgaatggc tttcgcgagt agctcttttc ccgtccccga ttccccaagc accatgaccg
     1261 tgctctcgct attggccacc atcggcagta ccgtgaagat ttcctgcatc ttcggacttc
     1321 ggctgatgat atcttcaaaa caatacttac catcgagttg ccgctgcaaa tgctcgagcg
     1381 gcgataaatc agatagtatc tccacccctc ccaagacatg ccccgtttcc gtcacgagaa
     1441 ttgctgtcgt caccatgggg gcgtccttta acgtatacat tgtcgctttc gcgcaactgc
     1501 ctgcg
//
